doc_id	sent_index	relation_id	relation	trigger	trigger_offset	arg_num	arg_head	arg_protein	arg_domain	arg_site	arg_sugar	arg_head_offset	arg_base_np	arg_base_np_offset	arg_np	arg_np_offset	sent_text
32935218	0	0	theme	red	76:78	arg1	alvarezii					101:109	the red seaweed (Kappaphycus alvarezii)	72:110	the red seaweed (Kappaphycus alvarezii)	72:110	Studies on the development and characterization of bioplastic film from the red seaweed (Kappaphycus alvarezii).
32935218	7	1	theme	wall	1095:1098	arg1	thickness					1100:1108	wall thickness	1095:1108	wall thickness of the film	1095:1120	Decrease in OTR was observed against decreasing wall thickness of the film.
32935218	5	2	theme	reaction	795:802	arg1	capabilities					804:815	the composition, phase transitions and chemical reaction capabilities	747:815	the composition, phase transitions and chemical reaction capabilities of the film	747:827	TG-DSC, AFM, SEM and FTIR spectroscopy analysis were performed to assess the composition, phase transitions and chemical reaction capabilities of the film.
32935218	6	3	dep	showed	896:901	arg1	whereas					946:952	whereas	946:952	whereas	946:952	The bioplastic film obtained from 4% K. alvarezii (whole seaweed) showed better physical and mechanical properties, whereas TG-DSC, FTIR and AFM showed similar kind of bioplastic properties in all the concentrations.
32935218	8	4	theme	potential	1178:1186	arg1	seaweed					1159:1165	seaweed	1159:1165	seaweed	1159:1165	The present study suggests that the seaweed would be a potential alternate source for bioplastic production which may reduce the usage of non-degradable plastics.
32935218	8	4	theme	potential	1178:1186	arg1	source					1198:1203	a potential alternate source	1176:1203	a potential alternate source for bioplastic production which may reduce the usage of non-degradable plastics	1176:1283	The present study suggests that the seaweed would be a potential alternate source for bioplastic production which may reduce the usage of non-degradable plastics.
32935218	6	5	theme	physical	910:917	arg1	properties					934:943	better physical and mechanical properties	903:943	better physical and mechanical properties	903:943	The bioplastic film obtained from 4% K. alvarezii (whole seaweed) showed better physical and mechanical properties, whereas TG-DSC, FTIR and AFM showed similar kind of bioplastic properties in all the concentrations.
32935218	0	6	from	Studies	0:6	arg1	characterization					31:46	characterization	31:46	characterization	31:46	Studies on the development and characterization of bioplastic film from the red seaweed (Kappaphycus alvarezii).
32935218	0	6	from	Studies	0:6	arg1	development					15:25	development	15:25	development	15:25	Studies on the development and characterization of bioplastic film from the red seaweed (Kappaphycus alvarezii).
32935218	5	7	theme	chemical	786:793	arg1	reaction					795:802	chemical reaction	786:802	chemical reaction	786:802	TG-DSC, AFM, SEM and FTIR spectroscopy analysis were performed to assess the composition, phase transitions and chemical reaction capabilities of the film.
32935218	0	8	theme	seaweed	80:86	arg1	alvarezii					101:109	the red seaweed (Kappaphycus alvarezii)	72:110	the red seaweed (Kappaphycus alvarezii)	72:110	Studies on the development and characterization of bioplastic film from the red seaweed (Kappaphycus alvarezii).
32935218	4	9	dep	colour	555:560	arg1	b					569:569	b	569:569	b	569:569	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	4	9	dep	colour	555:560	arg1	a					566:566	a	566:566	a	566:566	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	4	9	dep	colour	555:560	arg1	L					563:563	L	563:563	L	563:563	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	6	10	from	kind	990:993	arg1	concentrations					1031:1044	all the concentrations	1023:1044	all the concentrations	1023:1044	The bioplastic film obtained from 4% K. alvarezii (whole seaweed) showed better physical and mechanical properties, whereas TG-DSC, FTIR and AFM showed similar kind of bioplastic properties in all the concentrations.
32935218	2	11	theme	Kappaphycus	271:281	arg1	alvarezii					283:291	the red seaweed Kappaphycus alvarezii	255:291	the red seaweed Kappaphycus alvarezii	255:291	In this study, the red seaweed Kappaphycus alvarezii was used for bioplastic film production.
32935218	1	12	theme	rich	122:125	arg1	Seaweeds					113:120	Seaweeds	113:120	Seaweeds rich in polysaccharides	113:144	Seaweeds rich in polysaccharides are considered commercially important because of their wide range of industrial applications.
32935218	3	13	theme	polyethylene	346:357	arg1	glycol					359:364	Plasticizer polyethylene glycol	334:364	Plasticizer polyethylene glycol (PEG) to seaweed biomass ratio	334:395	Plasticizer polyethylene glycol (PEG) to seaweed biomass ratio was optimized and a thin bioplastic film with higher tensile strength was produced.
32935218	3	13	theme	polyethylene	346:357	arg1	PEG					367:369	PEG	367:369	PEG	367:369	Plasticizer polyethylene glycol (PEG) to seaweed biomass ratio was optimized and a thin bioplastic film with higher tensile strength was produced.
32935218	7	14	from	Decrease	1047:1054	arg1	OTR					1059:1061	OTR	1059:1061	OTR	1059:1061	Decrease in OTR was observed against decreasing wall thickness of the film.
32935218	1	15	from	polysaccharides	130:144	arg1	rich					122:125	rich	122:125	rich	122:125	Seaweeds rich in polysaccharides are considered commercially important because of their wide range of industrial applications.
32935218	2	16	theme	seaweed	263:269	arg1	alvarezii					283:291	the red seaweed Kappaphycus alvarezii	255:291	the red seaweed Kappaphycus alvarezii	255:291	In this study, the red seaweed Kappaphycus alvarezii was used for bioplastic film production.
32935218	5	17	theme	phase	764:768	arg1	transitions					770:780	phase transitions	764:780	phase transitions	764:780	TG-DSC, AFM, SEM and FTIR spectroscopy analysis were performed to assess the composition, phase transitions and chemical reaction capabilities of the film.
32935218	3	18	theme	bioplastic	422:431	arg1	film					433:436	a thin bioplastic film	415:436	a thin bioplastic film with higher tensile strength	415:465	Plasticizer polyethylene glycol (PEG) to seaweed biomass ratio was optimized and a thin bioplastic film with higher tensile strength was produced.
32935218	0	19	theme	Kappaphycus	89:99	arg1	alvarezii					101:109	the red seaweed (Kappaphycus alvarezii)	72:110	the red seaweed (Kappaphycus alvarezii)	72:110	Studies on the development and characterization of bioplastic film from the red seaweed (Kappaphycus alvarezii).
32935218	0	20	from	characterization	31:46	arg1	alvarezii					101:109	the red seaweed (Kappaphycus alvarezii)	72:110	the red seaweed (Kappaphycus alvarezii)	72:110	Studies on the development and characterization of bioplastic film from the red seaweed (Kappaphycus alvarezii).
32935218	5	21	theme	FTIR	695:698	arg1	analysis					713:720	FTIR spectroscopy analysis	695:720	FTIR spectroscopy analysis	695:720	TG-DSC, AFM, SEM and FTIR spectroscopy analysis were performed to assess the composition, phase transitions and chemical reaction capabilities of the film.
32935218	2	22	theme	red	259:261	arg1	alvarezii					283:291	the red seaweed Kappaphycus alvarezii	255:291	the red seaweed Kappaphycus alvarezii	255:291	In this study, the red seaweed Kappaphycus alvarezii was used for bioplastic film production.
32935218	3	23	theme	seaweed	375:381	arg1	ratio					391:395	seaweed biomass ratio	375:395	seaweed biomass ratio	375:395	Plasticizer polyethylene glycol (PEG) to seaweed biomass ratio was optimized and a thin bioplastic film with higher tensile strength was produced.
32935218	1	24	from	rich	122:125	arg1	polysaccharides					130:144	polysaccharides	130:144	polysaccharides	130:144	Seaweeds rich in polysaccharides are considered commercially important because of their wide range of industrial applications.
32935218	2	25	theme	bioplastic	306:315	arg1	production					322:331	bioplastic film production	306:331	bioplastic film production	306:331	In this study, the red seaweed Kappaphycus alvarezii was used for bioplastic film production.
32935218	4	26	theme	water	600:604	arg1	rate					626:629	water vapour transmission rate	600:629	water vapour transmission rate (WVTR)	600:636	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	4	26	theme	water	600:604	arg1	WVTR					632:635	WVTR	632:635	WVTR	632:635	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	3	27	theme	biomass	383:389	arg1	ratio					391:395	seaweed biomass ratio	375:395	seaweed biomass ratio	375:395	Plasticizer polyethylene glycol (PEG) to seaweed biomass ratio was optimized and a thin bioplastic film with higher tensile strength was produced.
32935218	4	28	theme	transmission	649:660	arg1	OTR					668:670	OTR	668:670	OTR	668:670	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	4	28	theme	transmission	649:660	arg1	rate					662:665	oxygen transmission rate	642:665	oxygen transmission rate (OTR)	642:671	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	3	29	theme	thin	417:420	arg1	film					433:436	a thin bioplastic film	415:436	a thin bioplastic film with higher tensile strength	415:465	Plasticizer polyethylene glycol (PEG) to seaweed biomass ratio was optimized and a thin bioplastic film with higher tensile strength was produced.
32935218	4	30	theme	tensile	537:543	arg1	strength					545:552	tensile strength	537:552	tensile strength	537:552	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	4	31	from	break	587:591	arg1	elongation					573:582	elongation	573:582	elongation at break (EAB)	573:597	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	4	31	from	break	587:591	arg1	strength					545:552	tensile strength	537:552	tensile strength	537:552	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	4	31	from	break	587:591	arg1	colour					555:560	colour	555:560	colour (L, a, b)	555:570	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	4	31	from	break	587:591	arg1	rate					626:629	water vapour transmission rate	600:629	water vapour transmission rate (WVTR)	600:636	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	4	31	from	break	587:591	arg1	rate					662:665	oxygen transmission rate	642:665	oxygen transmission rate (OTR)	642:671	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	4	31	from	break	587:591	arg1	EAB					594:596	EAB	594:596	EAB	594:596	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	4	31	from	break	587:591	arg1	thickness					526:534	its thickness	522:534	its thickness	522:534	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	4	31	from	break	587:591	arg1	OTR					668:670	OTR	668:670	OTR	668:670	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	4	31	from	break	587:591	arg1	WVTR					632:635	WVTR	632:635	WVTR	632:635	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	3	32	theme	tensile	450:456	arg1	strength					458:465	higher tensile strength	443:465	higher tensile strength	443:465	Plasticizer polyethylene glycol (PEG) to seaweed biomass ratio was optimized and a thin bioplastic film with higher tensile strength was produced.
32935218	1	33	theme	wide	201:204	arg1	range					206:210	their wide range	195:210	their wide range of industrial applications	195:237	Seaweeds rich in polysaccharides are considered commercially important because of their wide range of industrial applications.
32935218	6	34	theme	bioplastic	998:1007	arg1	properties					1009:1018	bioplastic properties	998:1018	bioplastic properties	998:1018	The bioplastic film obtained from 4% K. alvarezii (whole seaweed) showed better physical and mechanical properties, whereas TG-DSC, FTIR and AFM showed similar kind of bioplastic properties in all the concentrations.
32935218	6	35	theme	mechanical	923:932	arg1	properties					934:943	better physical and mechanical properties	903:943	better physical and mechanical properties	903:943	The bioplastic film obtained from 4% K. alvarezii (whole seaweed) showed better physical and mechanical properties, whereas TG-DSC, FTIR and AFM showed similar kind of bioplastic properties in all the concentrations.
32935218	8	36	theme	plastics	1276:1283	arg1	usage					1252:1256	the usage	1248:1256	the usage of non-degradable plastics	1248:1283	The present study suggests that the seaweed would be a potential alternate source for bioplastic production which may reduce the usage of non-degradable plastics.
32935218	5	37	theme	transitions	770:780	arg1	capabilities					804:815	the composition, phase transitions and chemical reaction capabilities	747:815	the composition, phase transitions and chemical reaction capabilities of the film	747:827	TG-DSC, AFM, SEM and FTIR spectroscopy analysis were performed to assess the composition, phase transitions and chemical reaction capabilities of the film.
32935218	1	38	theme	range	206:210	arg1	important					174:182	important	174:182	important	174:182	Seaweeds rich in polysaccharides are considered commercially important because of their wide range of industrial applications.
32935218	5	39	theme	composition	751:761	arg1	capabilities					804:815	the composition, phase transitions and chemical reaction capabilities	747:815	the composition, phase transitions and chemical reaction capabilities of the film	747:827	TG-DSC, AFM, SEM and FTIR spectroscopy analysis were performed to assess the composition, phase transitions and chemical reaction capabilities of the film.
32935218	8	40	theme	non-degradable	1261:1274	arg1	plastics					1276:1283	non-degradable plastics	1261:1283	non-degradable plastics	1261:1283	The present study suggests that the seaweed would be a potential alternate source for bioplastic production which may reduce the usage of non-degradable plastics.
32935218	3	41	theme	higher	443:448	arg1	strength					458:465	higher tensile strength	443:465	higher tensile strength	443:465	Plasticizer polyethylene glycol (PEG) to seaweed biomass ratio was optimized and a thin bioplastic film with higher tensile strength was produced.
32935218	0	42	theme	bioplastic	51:60	arg1	film					62:65	bioplastic film	51:65	bioplastic film from the red seaweed (Kappaphycus alvarezii)	51:110	Studies on the development and characterization of bioplastic film from the red seaweed (Kappaphycus alvarezii).
32935218	3	43	theme	Plasticizer	334:344	arg1	glycol					359:364	Plasticizer polyethylene glycol	334:364	Plasticizer polyethylene glycol (PEG) to seaweed biomass ratio	334:395	Plasticizer polyethylene glycol (PEG) to seaweed biomass ratio was optimized and a thin bioplastic film with higher tensile strength was produced.
32935218	3	43	theme	Plasticizer	334:344	arg1	PEG					367:369	PEG	367:369	PEG	367:369	Plasticizer polyethylene glycol (PEG) to seaweed biomass ratio was optimized and a thin bioplastic film with higher tensile strength was produced.
32935218	6	44	theme	similar	982:988	arg1	kind					990:993	similar kind	982:993	similar kind of bioplastic properties in all the concentrations	982:1044	The bioplastic film obtained from 4% K. alvarezii (whole seaweed) showed better physical and mechanical properties, whereas TG-DSC, FTIR and AFM showed similar kind of bioplastic properties in all the concentrations.
32935218	6	45	theme	properties	1009:1018	arg1	kind					990:993	similar kind	982:993	similar kind of bioplastic properties in all the concentrations	982:1044	The bioplastic film obtained from 4% K. alvarezii (whole seaweed) showed better physical and mechanical properties, whereas TG-DSC, FTIR and AFM showed similar kind of bioplastic properties in all the concentrations.
32935218	6	46	theme	bioplastic	834:843	arg1	film					845:848	The bioplastic film	830:848	The bioplastic film obtained from 4% K. alvarezii (whole seaweed)	830:894	The bioplastic film obtained from 4% K. alvarezii (whole seaweed) showed better physical and mechanical properties, whereas TG-DSC, FTIR and AFM showed similar kind of bioplastic properties in all the concentrations.
32935218	0	47	from	alvarezii	101:109	arg1	characterization					31:46	characterization	31:46	characterization	31:46	Studies on the development and characterization of bioplastic film from the red seaweed (Kappaphycus alvarezii).
32935218	0	47	from	alvarezii	101:109	arg1	film					62:65	bioplastic film	51:65	bioplastic film from the red seaweed (Kappaphycus alvarezii)	51:110	Studies on the development and characterization of bioplastic film from the red seaweed (Kappaphycus alvarezii).
32935218	0	47	from	alvarezii	101:109	arg1	development					15:25	development	15:25	development	15:25	Studies on the development and characterization of bioplastic film from the red seaweed (Kappaphycus alvarezii).
32935218	5	48	theme	film	824:827	arg1	capabilities					804:815	the composition, phase transitions and chemical reaction capabilities	747:815	the composition, phase transitions and chemical reaction capabilities of the film	747:827	TG-DSC, AFM, SEM and FTIR spectroscopy analysis were performed to assess the composition, phase transitions and chemical reaction capabilities of the film.
32935218	0	49	dep	development	15:25	arg1	the					11:13	the	11:13	the	11:13	Studies on the development and characterization of bioplastic film from the red seaweed (Kappaphycus alvarezii).
32935218	8	50	theme	present	1127:1133	arg1	study					1135:1139	The present study	1123:1139	The present study	1123:1139	The present study suggests that the seaweed would be a potential alternate source for bioplastic production which may reduce the usage of non-degradable plastics.
32935218	4	51	theme	oxygen	642:647	arg1	OTR					668:670	OTR	668:670	OTR	668:670	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	4	51	theme	oxygen	642:647	arg1	rate					662:665	oxygen transmission rate	642:665	oxygen transmission rate (OTR)	642:671	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	2	52	used	used	297:300	arg2	alvarezii					283:291	the red seaweed Kappaphycus alvarezii	255:291	the red seaweed Kappaphycus alvarezii	255:291	In this study, the red seaweed Kappaphycus alvarezii was used for bioplastic film production.
32935218	1	53	theme	industrial	215:224	arg1	applications					226:237	industrial applications	215:237	industrial applications	215:237	Seaweeds rich in polysaccharides are considered commercially important because of their wide range of industrial applications.
32935218	3	54	with	film	433:436	arg1	strength					458:465	higher tensile strength	443:465	higher tensile strength	443:465	Plasticizer polyethylene glycol (PEG) to seaweed biomass ratio was optimized and a thin bioplastic film with higher tensile strength was produced.
32935218	8	55	theme	alternate	1188:1196	arg1	seaweed					1159:1165	seaweed	1159:1165	seaweed	1159:1165	The present study suggests that the seaweed would be a potential alternate source for bioplastic production which may reduce the usage of non-degradable plastics.
32935218	8	55	theme	alternate	1188:1196	arg1	source					1198:1203	a potential alternate source	1176:1203	a potential alternate source for bioplastic production which may reduce the usage of non-degradable plastics	1176:1283	The present study suggests that the seaweed would be a potential alternate source for bioplastic production which may reduce the usage of non-degradable plastics.
32935218	2	56	theme	film	317:320	arg1	production					322:331	bioplastic film production	306:331	bioplastic film production	306:331	In this study, the red seaweed Kappaphycus alvarezii was used for bioplastic film production.
32935218	8	57	theme	bioplastic	1209:1218	arg1	production					1220:1229	bioplastic production	1209:1229	bioplastic production which may reduce the usage of non-degradable plastics	1209:1283	The present study suggests that the seaweed would be a potential alternate source for bioplastic production which may reduce the usage of non-degradable plastics.
32935218	1	58	theme	applications	226:237	arg1	range					206:210	their wide range	195:210	their wide range of industrial applications	195:237	Seaweeds rich in polysaccharides are considered commercially important because of their wide range of industrial applications.
32935218	6	59	dep	%	865:865	arg1	seaweed					887:893	seaweed	887:893	seaweed	887:893	The bioplastic film obtained from 4% K. alvarezii (whole seaweed) showed better physical and mechanical properties, whereas TG-DSC, FTIR and AFM showed similar kind of bioplastic properties in all the concentrations.
32935218	6	59	dep	%	865:865	arg1	K.					867:868	K.	867:868	K.	867:868	The bioplastic film obtained from 4% K. alvarezii (whole seaweed) showed better physical and mechanical properties, whereas TG-DSC, FTIR and AFM showed similar kind of bioplastic properties in all the concentrations.
32935218	4	60	theme	transmission	613:624	arg1	rate					626:629	water vapour transmission rate	600:629	water vapour transmission rate (WVTR)	600:636	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	4	60	theme	transmission	613:624	arg1	WVTR					632:635	WVTR	632:635	WVTR	632:635	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	0	61	theme	film	62:65	arg1	characterization					31:46	characterization	31:46	characterization	31:46	Studies on the development and characterization of bioplastic film from the red seaweed (Kappaphycus alvarezii).
32935218	0	61	theme	film	62:65	arg1	development					15:25	development	15:25	development	15:25	Studies on the development and characterization of bioplastic film from the red seaweed (Kappaphycus alvarezii).
32935218	0	62	from	development	15:25	arg1	alvarezii					101:109	the red seaweed (Kappaphycus alvarezii)	72:110	the red seaweed (Kappaphycus alvarezii)	72:110	Studies on the development and characterization of bioplastic film from the red seaweed (Kappaphycus alvarezii).
32935218	4	63	theme	vapour	606:611	arg1	rate					626:629	water vapour transmission rate	600:629	water vapour transmission rate (WVTR)	600:636	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	4	63	theme	vapour	606:611	arg1	WVTR					632:635	WVTR	632:635	WVTR	632:635	The films obtained were characterized by its thickness, tensile strength, colour (L, a, b), elongation at break (EAB), water vapour transmission rate (WVTR) and oxygen transmission rate (OTR).
32935218	7	64	theme	film	1117:1120	arg1	thickness					1100:1108	wall thickness	1095:1108	wall thickness of the film	1095:1120	Decrease in OTR was observed against decreasing wall thickness of the film.
32935218	5	65	theme	spectroscopy	700:711	arg1	analysis					713:720	FTIR spectroscopy analysis	695:720	FTIR spectroscopy analysis	695:720	TG-DSC, AFM, SEM and FTIR spectroscopy analysis were performed to assess the composition, phase transitions and chemical reaction capabilities of the film.
32172839	6	0	from	LL-2A	1013:1017	arg1	chains					990:995	mainly the side chains	974:995	mainly the side chains in the EPS from LL-2A	974:1017	Our data suggests that mainly the side chains in the EPS from LL-2A might be responsible for a higher ropiness than that observed for EPS from DGCC7919.
32172839	6	0	from	LL-2A	1013:1017	arg1	EPS					1004:1006	the EPS	1000:1006	the EPS from LL-2A	1000:1017	Our data suggests that mainly the side chains in the EPS from LL-2A might be responsible for a higher ropiness than that observed for EPS from DGCC7919.
32172839	6	0	from	LL-2A	1013:1017	arg1	responsible					1028:1038	responsible	1028:1038	responsible	1028:1038	Our data suggests that mainly the side chains in the EPS from LL-2A might be responsible for a higher ropiness than that observed for EPS from DGCC7919.
32172839	2	1	theme	EPS	334:336	arg1	properties					338:347	specific physical and chemical EPS properties	303:347	specific physical and chemical EPS properties	303:347	Some EPS cause ropiness which might be linked to specific physical and chemical EPS properties.
32172839	5	2	theme	Lactococcus	763:773	arg1	LL-2A					782:786	LL-2A	782:786	LL-2A	782:786	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	5	2	theme	Lactococcus	763:773	arg1	isolation					807:815	subsequent EPS isolation	792:815	subsequent EPS isolation	792:815	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	5	2	theme	Lactococcus	763:773	arg1	lactis					775:780	Lactococcus lactis	763:780	Lactococcus lactis LL-2A and subsequent EPS isolation	763:815	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	2	3	theme	chemical	325:332	arg1	properties					338:347	specific physical and chemical EPS properties	303:347	specific physical and chemical EPS properties	303:347	Some EPS cause ropiness which might be linked to specific physical and chemical EPS properties.
32172839	1	4	theme	product	237:243	arg1	texture					245:251	product texture	237:251	product texture	237:251	In the dairy industry, exopolysaccharides (EPS) produced in situ from lactic acid bacteria are of great interest because of their contribution to product texture.
32172839	3	5	theme	major	453:457	arg1	challenge					459:467	a major challenge	451:467	a major challenge to establish structure-function relationships	451:513	EPS show a broad variety of chemical structures and, because analysis is rather complex, it is still a major challenge to establish structure-function relationships.
32172839	3	5	theme	major	453:457	arg1	it					439:440	it	439:440	it	439:440	EPS show a broad variety of chemical structures and, because analysis is rather complex, it is still a major challenge to establish structure-function relationships.
32172839	4	6	theme	in-depth	600:607	arg1	elucidations					620:631	in-depth structural elucidations	600:631	in-depth structural elucidations	600:631	The aim of this study was to produce EPS with different degree of ropiness, perform in-depth structural elucidations and relate this information to their behaviour in aqueous solutions.
32172839	5	7	from	differences	884:894	arg1	composition					914:924	monosaccharide composition	899:924	monosaccharide composition	899:924	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	5	7	from	differences	884:894	arg1	linkages					941:948	glycosidic linkages	930:948	glycosidic linkages	930:948	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	3	8	theme	structure-function	482:499	arg1	relationships					501:513	structure-function relationships	482:513	structure-function relationships	482:513	EPS show a broad variety of chemical structures and, because analysis is rather complex, it is still a major challenge to establish structure-function relationships.
32172839	5	9	theme	macromolecular	842:855	arg1	properties					857:866	similar macromolecular properties	834:866	similar macromolecular properties	834:866	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	3	10	theme	chemical	378:385	arg1	structures					387:396	chemical structures	378:396	chemical structures	378:396	EPS show a broad variety of chemical structures and, because analysis is rather complex, it is still a major challenge to establish structure-function relationships.
32172839	5	11	theme	EPS	803:805	arg1	isolation					807:815	subsequent EPS isolation	792:815	subsequent EPS isolation	792:815	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	5	11	theme	EPS	803:805	arg1	lactis					775:780	Lactococcus lactis	763:780	Lactococcus lactis LL-2A and subsequent EPS isolation	763:815	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	1	12	theme	lactic	161:166	arg1	bacteria					173:180	lactic acid bacteria	161:180	lactic acid bacteria	161:180	In the dairy industry, exopolysaccharides (EPS) produced in situ from lactic acid bacteria are of great interest because of their contribution to product texture.
32172839	5	13	theme	subsequent	792:801	arg1	isolation					807:815	subsequent EPS isolation	792:815	subsequent EPS isolation	792:815	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	5	13	theme	subsequent	792:801	arg1	lactis					775:780	Lactococcus lactis	763:780	Lactococcus lactis LL-2A and subsequent EPS isolation	763:815	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	5	14	theme	Streptococcus	723:735	arg1	thermophilus					737:748	Streptococcus thermophilus DGCC7919	723:757	Streptococcus thermophilus DGCC7919	723:757	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	1	15	theme	acid	168:171	arg1	bacteria					173:180	lactic acid bacteria	161:180	lactic acid bacteria	161:180	In the dairy industry, exopolysaccharides (EPS) produced in situ from lactic acid bacteria are of great interest because of their contribution to product texture.
32172839	3	16	theme	broad	361:365	arg1	variety					367:373	a broad variety	359:373	a broad variety of chemical structures	359:396	EPS show a broad variety of chemical structures and, because analysis is rather complex, it is still a major challenge to establish structure-function relationships.
32172839	3	16	theme	broad	361:365	arg1	structures					387:396	chemical structures	378:396	chemical structures	378:396	EPS show a broad variety of chemical structures and, because analysis is rather complex, it is still a major challenge to establish structure-function relationships.
32172839	6	17	theme	side	985:988	arg1	chains					990:995	mainly the side chains	974:995	mainly the side chains in the EPS from LL-2A	974:1017	Our data suggests that mainly the side chains in the EPS from LL-2A might be responsible for a higher ropiness than that observed for EPS from DGCC7919.
32172839	6	17	theme	side	985:988	arg1	responsible					1028:1038	responsible	1028:1038	responsible	1028:1038	Our data suggests that mainly the side chains in the EPS from LL-2A might be responsible for a higher ropiness than that observed for EPS from DGCC7919.
32172839	5	18	dep	lactis	775:780	arg1	LL-2A					782:786	LL-2A	782:786	LL-2A	782:786	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	5	18	dep	lactis	775:780	arg1	isolation					807:815	subsequent EPS isolation	792:815	subsequent EPS isolation	792:815	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	5	18	dep	lactis	775:780	arg1	lactis					775:780	Lactococcus lactis	763:780	Lactococcus lactis LL-2A and subsequent EPS isolation	763:815	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	2	19	attach	linked	293:298	arg1	properties					338:347	specific physical and chemical EPS properties	303:347	specific physical and chemical EPS properties	303:347	Some EPS cause ropiness which might be linked to specific physical and chemical EPS properties.
32172839	2	19	attach	linked	293:298	arg2	ropiness					269:276	ropiness	269:276	ropiness which might be linked to specific physical and chemical EPS properties	269:347	Some EPS cause ropiness which might be linked to specific physical and chemical EPS properties.
32172839	1	20	theme	dairy	98:102	arg1	industry					104:111	the dairy industry	94:111	the dairy industry	94:111	In the dairy industry, exopolysaccharides (EPS) produced in situ from lactic acid bacteria are of great interest because of their contribution to product texture.
32172839	6	21	theme	higher	1046:1051	arg1	ropiness					1053:1060	a higher ropiness	1044:1060	a higher ropiness than that observed for EPS from DGCC7919	1044:1101	Our data suggests that mainly the side chains in the EPS from LL-2A might be responsible for a higher ropiness than that observed for EPS from DGCC7919.
32172839	0	22	theme	molecular	15:23	arg1	structure					25:33	molecular structure	15:33	molecular structure	15:33	Production and molecular structure of heteropolysaccharides from two lactic acid bacteria.
32172839	4	23	theme	study	532:536	arg1	aim					520:522	The aim	516:522	The aim of this study	516:536	The aim of this study was to produce EPS with different degree of ropiness, perform in-depth structural elucidations and relate this information to their behaviour in aqueous solutions.
32172839	5	24	theme	monosaccharide	899:912	arg1	composition					914:924	monosaccharide composition	899:924	monosaccharide composition	899:924	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	4	25	theme	ropiness	582:589	arg1	degree					572:577	different degree	562:577	different degree of ropiness	562:589	The aim of this study was to produce EPS with different degree of ropiness, perform in-depth structural elucidations and relate this information to their behaviour in aqueous solutions.
32172839	0	26	theme	heteropolysaccharides	38:58	arg1	Production					0:9	Production	0:9	Production	0:9	Production and molecular structure of heteropolysaccharides from two lactic acid bacteria.
32172839	0	26	theme	heteropolysaccharides	38:58	arg1	structure					25:33	molecular structure	15:33	molecular structure	15:33	Production and molecular structure of heteropolysaccharides from two lactic acid bacteria.
32172839	0	27	from	bacteria	81:88	arg1	Production					0:9	Production	0:9	Production	0:9	Production and molecular structure of heteropolysaccharides from two lactic acid bacteria.
32172839	0	27	from	bacteria	81:88	arg1	structure					25:33	molecular structure	15:33	molecular structure	15:33	Production and molecular structure of heteropolysaccharides from two lactic acid bacteria.
32172839	4	28	from	behaviour	670:678	arg1	solutions					691:699	aqueous solutions	683:699	aqueous solutions	683:699	The aim of this study was to produce EPS with different degree of ropiness, perform in-depth structural elucidations and relate this information to their behaviour in aqueous solutions.
32172839	1	29	theme	great	189:193	arg1	interest					195:202	great interest	189:202	great interest	189:202	In the dairy industry, exopolysaccharides (EPS) produced in situ from lactic acid bacteria are of great interest because of their contribution to product texture.
32172839	3	30	theme	structures	387:396	arg1	variety					367:373	a broad variety	359:373	a broad variety of chemical structures	359:396	EPS show a broad variety of chemical structures and, because analysis is rather complex, it is still a major challenge to establish structure-function relationships.
32172839	3	30	theme	structures	387:396	arg1	structures					387:396	chemical structures	378:396	chemical structures	378:396	EPS show a broad variety of chemical structures and, because analysis is rather complex, it is still a major challenge to establish structure-function relationships.
32172839	5	31	theme	similar	834:840	arg1	properties					857:866	similar macromolecular properties	834:866	similar macromolecular properties	834:866	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	5	32	theme	thermophilus	737:748	arg1	cultivation					708:718	cultivation	708:718	cultivation of Streptococcus thermophilus DGCC7919	708:757	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	5	32	theme	thermophilus	737:748	arg1	LL-2A					782:786	LL-2A	782:786	LL-2A	782:786	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	5	32	theme	thermophilus	737:748	arg1	isolation					807:815	subsequent EPS isolation	792:815	subsequent EPS isolation	792:815	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	5	32	theme	thermophilus	737:748	arg1	lactis					775:780	Lactococcus lactis	763:780	Lactococcus lactis LL-2A and subsequent EPS isolation	763:815	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	1	33	from	interest	195:202	arg1	industry					104:111	the dairy industry	94:111	the dairy industry	94:111	In the dairy industry, exopolysaccharides (EPS) produced in situ from lactic acid bacteria are of great interest because of their contribution to product texture.
32172839	6	34	from	chains	990:995	arg1	LL-2A					1013:1017	LL-2A	1013:1017	LL-2A	1013:1017	Our data suggests that mainly the side chains in the EPS from LL-2A might be responsible for a higher ropiness than that observed for EPS from DGCC7919.
32172839	6	34	from	chains	990:995	arg1	EPS					1004:1006	the EPS	1000:1006	the EPS from LL-2A	1000:1017	Our data suggests that mainly the side chains in the EPS from LL-2A might be responsible for a higher ropiness than that observed for EPS from DGCC7919.
32172839	2	35	theme	physical	312:319	arg1	properties					338:347	specific physical and chemical EPS properties	303:347	specific physical and chemical EPS properties	303:347	Some EPS cause ropiness which might be linked to specific physical and chemical EPS properties.
32172839	4	36	with	EPS	553:555	arg1	degree					572:577	different degree	562:577	different degree of ropiness	562:589	The aim of this study was to produce EPS with different degree of ropiness, perform in-depth structural elucidations and relate this information to their behaviour in aqueous solutions.
32172839	2	37	theme	specific	303:310	arg1	properties					338:347	specific physical and chemical EPS properties	303:347	specific physical and chemical EPS properties	303:347	Some EPS cause ropiness which might be linked to specific physical and chemical EPS properties.
32172839	4	38	theme	different	562:570	arg1	degree					572:577	different degree	562:577	different degree of ropiness	562:589	The aim of this study was to produce EPS with different degree of ropiness, perform in-depth structural elucidations and relate this information to their behaviour in aqueous solutions.
32172839	4	39	theme	aqueous	683:689	arg1	solutions					691:699	aqueous solutions	683:699	aqueous solutions	683:699	The aim of this study was to produce EPS with different degree of ropiness, perform in-depth structural elucidations and relate this information to their behaviour in aqueous solutions.
32172839	5	40	theme	glycosidic	930:939	arg1	linkages					941:948	glycosidic linkages	930:948	glycosidic linkages	930:948	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	0	41	theme	acid	76:79	arg1	bacteria					81:88	two lactic acid bacteria	65:88	two lactic acid bacteria	65:88	Production and molecular structure of heteropolysaccharides from two lactic acid bacteria.
32172839	5	42	theme	pronounced	873:882	arg1	differences					884:894	pronounced differences	873:894	pronounced differences in monosaccharide composition and glycosidic linkages	873:948	After cultivation of Streptococcus thermophilus DGCC7919 and Lactococcus lactis LL-2A and subsequent EPS isolation, both EPS showed similar macromolecular properties, but pronounced differences in monosaccharide composition and glycosidic linkages.
32172839	4	43	theme	structural	609:618	arg1	elucidations					620:631	in-depth structural elucidations	600:631	in-depth structural elucidations	600:631	The aim of this study was to produce EPS with different degree of ropiness, perform in-depth structural elucidations and relate this information to their behaviour in aqueous solutions.
32172839	0	44	theme	lactic	69:74	arg1	bacteria					81:88	two lactic acid bacteria	65:88	two lactic acid bacteria	65:88	Production and molecular structure of heteropolysaccharides from two lactic acid bacteria.
32172839	6	45	from	DGCC7919	1094:1101	arg1	EPS					1085:1087	EPS	1085:1087	EPS from DGCC7919	1085:1101	Our data suggests that mainly the side chains in the EPS from LL-2A might be responsible for a higher ropiness than that observed for EPS from DGCC7919.
33419097	8	0	theme	enzymatic	969:977	arg1	assays					979:984	The enzymatic assays	965:984	The enzymatic assays	965:984	The enzymatic assays revealed no influence of CPS on xanthine oxidase and the inhibition of catalase activity.
33419097	10	1	theme	chemopreventive	1425:1439	arg1	agent					1457:1461	chemopreventive and therapeutic agent	1425:1461	chemopreventive and therapeutic agent in more invasive stages of this type of cancer	1425:1508	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	1	2	theme	study	131:135	arg1	aim					112:114	The aim	108:114	The aim of the present study	108:135	The aim of the present study was to evaluate in vitro the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer.
33419097	10	3	theme	regular	1248:1254	arg1	diet					1262:1265	the regular human diet	1244:1265	the regular human diet	1244:1265	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	0	4	from	Potential	10:18	arg1	crispa					60:65	Sparassis crispa	50:65	Sparassis crispa	50:65	Promising Potential of Crude Polysaccharides from Sparassis crispa against Colon Cancer: An In Vitro Study.
33419097	10	5	theme	promising	1345:1353	arg1	activities					1355:1364	promising activities	1345:1364	promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer	1345:1508	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	7	6	theme	various	905:911	arg1	methods					913:919	various methods	905:919	various methods	905:919	Antioxidant activity was determined by various methods and revealed the moderate potential of CPS.
33419097	3	7	theme	1H	454:455	arg1	NMR					457:459	1H NMR	454:459	1H NMR	454:459	1H NMR and GC-MS methods were used for the structural analysis of CPS.
33419097	10	8	theme	potential	1408:1416	arg1	use					1418:1420	its potential use	1404:1420	its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer	1404:1508	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	0	9	theme	In	92:93	arg1	Study					101:105	An In Vitro Study	89:105	Promising Potential of Crude Polysaccharides from Sparassis crispa against Colon Cancer: An In Vitro Study.	0:106	Promising Potential of Crude Polysaccharides from Sparassis crispa against Colon Cancer: An In Vitro Study.
33419097	7	10	theme	Antioxidant	866:876	arg1	activity					878:885	Antioxidant activity	866:885	Antioxidant activity	866:885	Antioxidant activity was determined by various methods and revealed the moderate potential of CPS.
33419097	2	11	attach	present	423:429	arg2	phenolics					409:417	phenolics	409:417	phenolics	409:417	The determination of the chemical composition of CPS has revealed that it contains mostly carbohydrates, while proteins or phenolics are present only in trace amounts.
33419097	2	11	attach	present	423:429	arg1	amounts					445:451	trace amounts	439:451	trace amounts	439:451	The determination of the chemical composition of CPS has revealed that it contains mostly carbohydrates, while proteins or phenolics are present only in trace amounts.
33419097	2	11	attach	present	423:429	arg2	proteins					397:404	proteins	397:404	proteins	397:404	The determination of the chemical composition of CPS has revealed that it contains mostly carbohydrates, while proteins or phenolics are present only in trace amounts.
33419097	4	12	theme	Biological	525:534	arg1	activity					536:543	Biological activity	525:543	Biological activity including anticancer, anti-inflammatory and antioxidant properties of CPS	525:617	Biological activity including anticancer, anti-inflammatory and antioxidant properties of CPS was investigated.
33419097	5	13	theme	human	677:681	arg1	cells					711:715	normal human colon epithelial CCD841 CoN cells	670:715	normal human colon epithelial CCD841 CoN cells	670:715	CPS was found to be non-toxic to normal human colon epithelial CCD841 CoN cells.
33419097	0	14	from	crispa	60:65	arg1	Potential					10:18	Promising Potential	0:18	Promising Potential of Crude Polysaccharides from Sparassis crispa against Colon Cancer: An In Vitro Study.	0:106	Promising Potential of Crude Polysaccharides from Sparassis crispa against Colon Cancer: An In Vitro Study.
33419097	0	14	from	crispa	60:65	arg1	Polysaccharides					29:43	Crude Polysaccharides	23:43	Crude Polysaccharides from Sparassis crispa	23:65	Promising Potential of Crude Polysaccharides from Sparassis crispa against Colon Cancer: An In Vitro Study.
33419097	10	15	theme	valuable	1227:1234	arg1	crispa					1215:1220	S. crispa	1212:1220	S. crispa	1212:1220	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	10	15	theme	valuable	1227:1234	arg1	part					1236:1239	a valuable part	1225:1239	a valuable part	1225:1239	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	8	16	theme	activity	1066:1073	arg1	oxidase					1027:1033	xanthine oxidase	1018:1033	xanthine oxidase	1018:1033	The enzymatic assays revealed no influence of CPS on xanthine oxidase and the inhibition of catalase activity.
33419097	8	16	theme	activity	1066:1073	arg1	inhibition					1043:1052	the inhibition	1039:1052	the inhibition of catalase activity	1039:1073	The enzymatic assays revealed no influence of CPS on xanthine oxidase and the inhibition of catalase activity.
33419097	5	17	theme	colon	683:687	arg1	cells					711:715	normal human colon epithelial CCD841 CoN cells	670:715	normal human colon epithelial CCD841 CoN cells	670:715	CPS was found to be non-toxic to normal human colon epithelial CCD841 CoN cells.
33419097	10	18	theme	cancer	1325:1330	arg1	risk					1311:1314	the risk	1307:1314	the risk of colon cancer	1307:1330	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	4	19	theme	anti-inflammatory	567:583	arg1	properties					601:610	anti-inflammatory and antioxidant properties	567:610	anti-inflammatory and antioxidant properties of CPS	567:617	Biological activity including anticancer, anti-inflammatory and antioxidant properties of CPS was investigated.
33419097	4	19	theme	anti-inflammatory	567:583	arg1	anticancer					555:564	anticancer	555:564	anticancer	555:564	Biological activity including anticancer, anti-inflammatory and antioxidant properties of CPS was investigated.
33419097	0	20	dep	In	92:93	arg1	Vitro					95:99	Vitro	95:99	Vitro	95:99	Promising Potential of Crude Polysaccharides from Sparassis crispa against Colon Cancer: An In Vitro Study.
33419097	3	21	used	used	484:487	arg2	GC-MS					465:469	GC-MS	465:469	GC-MS	465:469	1H NMR and GC-MS methods were used for the structural analysis of CPS.
33419097	3	21	used	used	484:487	arg2	NMR					457:459	1H NMR	454:459	1H NMR	454:459	1H NMR and GC-MS methods were used for the structural analysis of CPS.
33419097	4	22	theme	CPS	615:617	arg1	properties					601:610	anti-inflammatory and antioxidant properties	567:610	anti-inflammatory and antioxidant properties of CPS	567:617	Biological activity including anticancer, anti-inflammatory and antioxidant properties of CPS was investigated.
33419097	4	22	theme	CPS	615:617	arg1	anticancer					555:564	anticancer	555:564	anticancer	555:564	Biological activity including anticancer, anti-inflammatory and antioxidant properties of CPS was investigated.
33419097	5	23	theme	epithelial	689:698	arg1	cells					711:715	normal human colon epithelial CCD841 CoN cells	670:715	normal human colon epithelial CCD841 CoN cells	670:715	CPS was found to be non-toxic to normal human colon epithelial CCD841 CoN cells.
33419097	2	24	from	present	423:429	arg1	amounts					445:451	trace amounts	439:451	trace amounts	439:451	The determination of the chemical composition of CPS has revealed that it contains mostly carbohydrates, while proteins or phenolics are present only in trace amounts.
33419097	8	25	from	influence	998:1006	arg1	oxidase					1027:1033	xanthine oxidase	1018:1033	xanthine oxidase	1018:1033	The enzymatic assays revealed no influence of CPS on xanthine oxidase and the inhibition of catalase activity.
33419097	8	25	from	influence	998:1006	arg1	inhibition					1043:1052	the inhibition	1039:1052	the inhibition of catalase activity	1039:1073	The enzymatic assays revealed no influence of CPS on xanthine oxidase and the inhibition of catalase activity.
33419097	6	26	theme	cell	829:832	arg1	LS180					849:853	LS180	849:853	LS180	849:853	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	6	26	theme	cell	829:832	arg1	Caco-2					841:846	Caco-2	841:846	Caco-2	841:846	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	6	26	theme	cell	829:832	arg1	lines					834:838	human colon cancer cell lines	810:838	human colon cancer cell lines: Caco-2, LS180 and HT-29	810:863	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	6	26	theme	cell	829:832	arg1	HT-29					859:863	HT-29	859:863	HT-29	859:863	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	8	27	theme	CPS	1011:1013	arg1	influence					998:1006	no influence	995:1006	no influence of CPS on xanthine oxidase and the inhibition of catalase activity	995:1073	The enzymatic assays revealed no influence of CPS on xanthine oxidase and the inhibition of catalase activity.
33419097	5	28	theme	CCD841	700:705	arg1	cells					711:715	normal human colon epithelial CCD841 CoN cells	670:715	normal human colon epithelial CCD841 CoN cells	670:715	CPS was found to be non-toxic to normal human colon epithelial CCD841 CoN cells.
33419097	3	29	theme	structural	497:506	arg1	analysis					508:515	the structural analysis	493:515	the structural analysis of CPS	493:522	1H NMR and GC-MS methods were used for the structural analysis of CPS.
33419097	2	30	theme	CPS	335:337	arg1	composition					320:330	the chemical composition	307:330	the chemical composition of CPS	307:337	The determination of the chemical composition of CPS has revealed that it contains mostly carbohydrates, while proteins or phenolics are present only in trace amounts.
33419097	0	31	theme	Promising	0:8	arg1	Potential					10:18	Promising Potential	0:18	Promising Potential of Crude Polysaccharides from Sparassis crispa against Colon Cancer: An In Vitro Study.	0:106	Promising Potential of Crude Polysaccharides from Sparassis crispa against Colon Cancer: An In Vitro Study.
33419097	10	32	theme	colon	1319:1323	arg1	cancer					1325:1330	colon cancer	1319:1330	colon cancer	1319:1330	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	5	33	theme	normal	670:675	arg1	cells					711:715	normal human colon epithelial CCD841 CoN cells	670:715	normal human colon epithelial CCD841 CoN cells	670:715	CPS was found to be non-toxic to normal human colon epithelial CCD841 CoN cells.
33419097	0	34	theme	Crude	23:27	arg1	Polysaccharides					29:43	Crude Polysaccharides	23:43	Crude Polysaccharides from Sparassis crispa	23:65	Promising Potential of Crude Polysaccharides from Sparassis crispa against Colon Cancer: An In Vitro Study.
33419097	8	35	theme	xanthine	1018:1025	arg1	oxidase					1027:1033	xanthine oxidase	1018:1033	xanthine oxidase	1018:1033	The enzymatic assays revealed no influence of CPS on xanthine oxidase and the inhibition of catalase activity.
33419097	6	36	theme	membrane	749:756	arg1	integrity					758:766	membrane integrity	749:766	membrane integrity	749:766	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	3	37	dep	NMR	457:459	arg1	methods					471:477	methods	471:477	methods	471:477	1H NMR and GC-MS methods were used for the structural analysis of CPS.
33419097	10	38	theme	further	1378:1384	arg1	studies					1386:1392	further studies	1378:1392	further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer	1378:1508	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	10	39	theme	cancer	1503:1508	arg1	type					1495:1498	this type	1490:1498	this type of cancer	1490:1508	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	6	40	theme	cancer	822:827	arg1	LS180					849:853	LS180	849:853	LS180	849:853	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	6	40	theme	cancer	822:827	arg1	Caco-2					841:846	Caco-2	841:846	Caco-2	841:846	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	6	40	theme	cancer	822:827	arg1	lines					834:838	human colon cancer cell lines	810:838	human colon cancer cell lines: Caco-2, LS180 and HT-29	810:863	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	6	40	theme	cancer	822:827	arg1	HT-29					859:863	HT-29	859:863	HT-29	859:863	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	10	41	theme	invasive	1471:1478	arg1	stages					1480:1485	more invasive stages	1466:1485	more invasive stages of this type of cancer	1466:1508	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	1	42	theme	beneficial	166:175	arg1	potential					177:185	the beneficial potential	162:185	the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer	162:283	The aim of the present study was to evaluate in vitro the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer.
33419097	10	43	from	reduction	1294:1302	arg1	risk					1311:1314	the risk	1307:1314	the risk of colon cancer	1307:1330	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	1	44	from	crispa	220:225	arg1	potential					177:185	the beneficial potential	162:185	the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer	162:283	The aim of the present study was to evaluate in vitro the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer.
33419097	1	44	from	crispa	220:225	arg1	polysaccharides					196:210	crude polysaccharides	190:210	crude polysaccharides from S. crispa (CPS)	190:231	The aim of the present study was to evaluate in vitro the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer.
33419097	6	45	theme	colon	816:820	arg1	LS180					849:853	LS180	849:853	LS180	849:853	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	6	45	theme	colon	816:820	arg1	Caco-2					841:846	Caco-2	841:846	Caco-2	841:846	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	6	45	theme	colon	816:820	arg1	lines					834:838	human colon cancer cell lines	810:838	human colon cancer cell lines: Caco-2, LS180 and HT-29	810:863	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	6	45	theme	colon	816:820	arg1	HT-29					859:863	HT-29	859:863	HT-29	859:863	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	10	46	from	agent	1457:1461	arg1	stages					1480:1485	more invasive stages	1466:1485	more invasive stages of this type of cancer	1466:1508	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	0	47	theme	Polysaccharides	29:43	arg1	Potential					10:18	Promising Potential	0:18	Promising Potential of Crude Polysaccharides from Sparassis crispa against Colon Cancer: An In Vitro Study.	0:106	Promising Potential of Crude Polysaccharides from Sparassis crispa against Colon Cancer: An In Vitro Study.
33419097	6	48	dep	lines	834:838	arg1	LS180					849:853	LS180	849:853	LS180	849:853	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	6	48	dep	lines	834:838	arg1	Caco-2					841:846	Caco-2	841:846	Caco-2	841:846	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	6	48	dep	lines	834:838	arg1	lines					834:838	human colon cancer cell lines	810:838	human colon cancer cell lines: Caco-2, LS180 and HT-29	810:863	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	6	48	dep	lines	834:838	arg1	HT-29					859:863	HT-29	859:863	HT-29	859:863	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	2	49	from	amounts	445:451	arg1	present					423:429	present	423:429	present	423:429	The determination of the chemical composition of CPS has revealed that it contains mostly carbohydrates, while proteins or phenolics are present only in trace amounts.
33419097	1	50	from	potential	177:185	arg1	cancer					278:283	the most common cancer types-colon cancer	243:283	the most common cancer types-colon cancer	243:283	The aim of the present study was to evaluate in vitro the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer.
33419097	1	50	from	potential	177:185	arg1	CPS					228:230	CPS	228:230	CPS	228:230	The aim of the present study was to evaluate in vitro the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer.
33419097	1	50	from	potential	177:185	arg1	one					236:238	one	236:238	one	236:238	The aim of the present study was to evaluate in vitro the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer.
33419097	1	50	from	potential	177:185	arg1	crispa					220:225	S. crispa	217:225	S. crispa (CPS)	217:231	The aim of the present study was to evaluate in vitro the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer.
33419097	4	51	theme	antioxidant	589:599	arg1	properties					601:610	anti-inflammatory and antioxidant properties	567:610	anti-inflammatory and antioxidant properties of CPS	567:617	Biological activity including anticancer, anti-inflammatory and antioxidant properties of CPS was investigated.
33419097	4	51	theme	antioxidant	589:599	arg1	anticancer					555:564	anticancer	555:564	anticancer	555:564	Biological activity including anticancer, anti-inflammatory and antioxidant properties of CPS was investigated.
33419097	1	52	theme	common	252:257	arg1	cancer					278:283	the most common cancer types-colon cancer	243:283	the most common cancer types-colon cancer	243:283	The aim of the present study was to evaluate in vitro the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer.
33419097	5	53	theme	CoN	707:709	arg1	cells					711:715	normal human colon epithelial CCD841 CoN cells	670:715	normal human colon epithelial CCD841 CoN cells	670:715	CPS was found to be non-toxic to normal human colon epithelial CCD841 CoN cells.
33419097	6	54	theme	lines	834:838	arg1	proliferation					793:805	the proliferation	789:805	the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29	789:863	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	0	55	theme	Sparassis	50:58	arg1	crispa					60:65	Sparassis crispa	50:65	Sparassis crispa	50:65	Promising Potential of Crude Polysaccharides from Sparassis crispa against Colon Cancer: An In Vitro Study.
33419097	1	56	theme	cancer	259:264	arg1	cancer					278:283	the most common cancer types-colon cancer	243:283	the most common cancer types-colon cancer	243:283	The aim of the present study was to evaluate in vitro the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer.
33419097	10	57	theme	therapeutic	1445:1455	arg1	agent					1457:1461	chemopreventive and therapeutic agent	1425:1461	chemopreventive and therapeutic agent in more invasive stages of this type of cancer	1425:1508	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	3	58	theme	CPS	520:522	arg1	analysis					508:515	the structural analysis	493:515	the structural analysis of CPS	493:522	1H NMR and GC-MS methods were used for the structural analysis of CPS.
33419097	10	59	theme	diet	1262:1265	arg1	crispa					1215:1220	S. crispa	1212:1220	S. crispa	1212:1220	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	10	59	theme	diet	1262:1265	arg1	part					1236:1239	a valuable part	1225:1239	a valuable part	1225:1239	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	6	60	theme	human	810:814	arg1	LS180					849:853	LS180	849:853	LS180	849:853	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	6	60	theme	human	810:814	arg1	Caco-2					841:846	Caco-2	841:846	Caco-2	841:846	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	6	60	theme	human	810:814	arg1	lines					834:838	human colon cancer cell lines	810:838	human colon cancer cell lines: Caco-2, LS180 and HT-29	810:863	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	6	60	theme	human	810:814	arg1	HT-29					859:863	HT-29	859:863	HT-29	859:863	Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29.
33419097	1	61	theme	types-colon	266:276	arg1	cancer					278:283	the most common cancer types-colon cancer	243:283	the most common cancer types-colon cancer	243:283	The aim of the present study was to evaluate in vitro the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer.
33419097	8	62	theme	catalase	1057:1064	arg1	activity					1066:1073	catalase activity	1057:1073	catalase activity	1057:1073	The enzymatic assays revealed no influence of CPS on xanthine oxidase and the inhibition of catalase activity.
33419097	10	63	theme	type	1495:1498	arg1	stages					1480:1485	more invasive stages	1466:1485	more invasive stages of this type of cancer	1466:1508	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	2	64	located	present	423:429	arg2	phenolics					409:417	phenolics	409:417	phenolics	409:417	The determination of the chemical composition of CPS has revealed that it contains mostly carbohydrates, while proteins or phenolics are present only in trace amounts.
33419097	2	64	located	present	423:429	arg1	amounts					445:451	trace amounts	439:451	trace amounts	439:451	The determination of the chemical composition of CPS has revealed that it contains mostly carbohydrates, while proteins or phenolics are present only in trace amounts.
33419097	2	64	located	present	423:429	arg2	proteins					397:404	proteins	397:404	proteins	397:404	The determination of the chemical composition of CPS has revealed that it contains mostly carbohydrates, while proteins or phenolics are present only in trace amounts.
33419097	1	65	theme	crude	190:194	arg1	polysaccharides					196:210	crude polysaccharides	190:210	crude polysaccharides from S. crispa (CPS)	190:231	The aim of the present study was to evaluate in vitro the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer.
33419097	10	66	theme	human	1256:1260	arg1	diet					1262:1265	the regular human diet	1244:1265	the regular human diet	1244:1265	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	2	67	contain	contains	360:367	arg1	it					357:358	it	357:358	it	357:358	The determination of the chemical composition of CPS has revealed that it contains mostly carbohydrates, while proteins or phenolics are present only in trace amounts.
33419097	2	67	contain	contains	360:367	arg2	carbohydrates					376:388	carbohydrates	376:388	carbohydrates	376:388	The determination of the chemical composition of CPS has revealed that it contains mostly carbohydrates, while proteins or phenolics are present only in trace amounts.
33419097	7	68	theme	CPS	960:962	arg1	potential					947:955	the moderate potential	934:955	the moderate potential of CPS	934:962	Antioxidant activity was determined by various methods and revealed the moderate potential of CPS.
33419097	9	69	theme	pro-inflammatory	1086:1101	arg1	lipooxygenase					1139:1151	lipooxygenase	1139:1151	lipooxygenase	1139:1151	Moreover, pro-inflammatory enzymes such as cyclooxygenase-2 or lipooxygenase were inhibited by CPS.
33419097	9	69	theme	pro-inflammatory	1086:1101	arg1	enzymes					1103:1109	pro-inflammatory enzymes	1086:1109	pro-inflammatory enzymes such as cyclooxygenase-2 or lipooxygenase	1086:1151	Moreover, pro-inflammatory enzymes such as cyclooxygenase-2 or lipooxygenase were inhibited by CPS.
33419097	9	69	theme	pro-inflammatory	1086:1101	arg1	cyclooxygenase-2					1119:1134	cyclooxygenase-2	1119:1134	cyclooxygenase-2	1119:1134	Moreover, pro-inflammatory enzymes such as cyclooxygenase-2 or lipooxygenase were inhibited by CPS.
33419097	2	70	theme	chemical	311:318	arg1	composition					320:330	the chemical composition	307:330	the chemical composition of CPS	307:337	The determination of the chemical composition of CPS has revealed that it contains mostly carbohydrates, while proteins or phenolics are present only in trace amounts.
33419097	1	71	theme	polysaccharides	196:210	arg1	potential					177:185	the beneficial potential	162:185	the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer	162:283	The aim of the present study was to evaluate in vitro the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer.
33419097	0	72	theme	Colon	75:79	arg1	Cancer					81:86	Colon Cancer	75:86	Colon Cancer	75:86	Promising Potential of Crude Polysaccharides from Sparassis crispa against Colon Cancer: An In Vitro Study.
33419097	0	73	dep	Potential	10:18	arg1	Study					101:105	An In Vitro Study	89:105	Promising Potential of Crude Polysaccharides from Sparassis crispa against Colon Cancer: An In Vitro Study.	0:106	Promising Potential of Crude Polysaccharides from Sparassis crispa against Colon Cancer: An In Vitro Study.
33419097	7	74	theme	moderate	938:945	arg1	potential					947:955	the moderate potential	934:955	the moderate potential of CPS	934:962	Antioxidant activity was determined by various methods and revealed the moderate potential of CPS.
33419097	2	75	theme	composition	320:330	arg1	determination					290:302	The determination	286:302	The determination of the chemical composition of CPS	286:337	The determination of the chemical composition of CPS has revealed that it contains mostly carbohydrates, while proteins or phenolics are present only in trace amounts.
33419097	10	76	contain	possess	1337:1343	arg1	crispa					1215:1220	S. crispa	1212:1220	S. crispa	1212:1220	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	10	76	contain	possess	1337:1343	arg2	activities					1355:1364	promising activities	1345:1364	promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer	1345:1508	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	10	76	contain	possess	1337:1343	arg1	part					1236:1239	a valuable part	1225:1239	a valuable part	1225:1239	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	2	77	theme	trace	439:443	arg1	amounts					445:451	trace amounts	439:451	trace amounts	439:451	The determination of the chemical composition of CPS has revealed that it contains mostly carbohydrates, while proteins or phenolics are present only in trace amounts.
33419097	1	78	theme	cancer	278:283	arg1	cancer					278:283	the most common cancer types-colon cancer	243:283	the most common cancer types-colon cancer	243:283	The aim of the present study was to evaluate in vitro the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer.
33419097	1	78	theme	cancer	278:283	arg1	one					236:238	one	236:238	one	236:238	The aim of the present study was to evaluate in vitro the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer.
33419097	1	79	theme	present	123:129	arg1	study					131:135	the present study	119:135	the present study	119:135	The aim of the present study was to evaluate in vitro the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer.
33419097	10	80	theme	S.	1212:1213	arg1	crispa					1215:1220	S. crispa	1212:1220	S. crispa	1212:1220	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	10	80	theme	S.	1212:1213	arg1	part					1236:1239	a valuable part	1225:1239	a valuable part	1225:1239	Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.
33419097	1	81	theme	S.	217:218	arg1	CPS					228:230	CPS	228:230	CPS	228:230	The aim of the present study was to evaluate in vitro the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer.
33419097	1	81	theme	S.	217:218	arg1	crispa					220:225	S. crispa	217:225	S. crispa (CPS)	217:231	The aim of the present study was to evaluate in vitro the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types-colon cancer.
34049138	5	0	from	CFUm-3	898:903	arg1	reduction					820:828	A significant reduction	806:828	A significant reduction in culturable bacteria of aerosol (from 6.23 log CFUm-3 to 2.33 log CFUm-3)	806:904	A significant reduction in culturable bacteria of aerosol (from 6.23 log CFUm-3 to 2.33 log CFUm-3) was observed in presence of cellulose nanofibers and chitosan composites.
34049138	4	1	theme	citrals	789:795	arg1	content					797:803	citrals content	789:803	citrals content	789:803	The composite system had high encapsulation efficiency (88-91%) and citrals content.
34049138	3	2	theme	different	666:674	arg1	fractions					681:689	different size fractions	666:689	different size fractions of aerosol (<0.25 μm-2.5 μm)	666:718	The evaluation of the inhibitory effect of the composite system on culturable bacteria of the indoor air was done at different sites (air volume from 30 m3 to 80 m3) and in different size fractions of aerosol (<0.25 μm-2.5 μm).
34049138	3	3	theme	effect	526:531	arg1	evaluation					497:506	The evaluation	493:506	The evaluation of the inhibitory effect of the composite system on culturable bacteria of the indoor air	493:596	The evaluation of the inhibitory effect of the composite system on culturable bacteria of the indoor air was done at different sites (air volume from 30 m3 to 80 m3) and in different size fractions of aerosol (<0.25 μm-2.5 μm).
34049138	5	4	theme	significant	808:818	arg1	reduction					820:828	A significant reduction	806:828	A significant reduction in culturable bacteria of aerosol (from 6.23 log CFUm-3 to 2.33 log CFUm-3)	806:904	A significant reduction in culturable bacteria of aerosol (from 6.23 log CFUm-3 to 2.33 log CFUm-3) was observed in presence of cellulose nanofibers and chitosan composites.
34049138	9	5	theme	compounds	1599:1607	arg1	interaction					1570:1580	the interaction	1566:1580	the interaction of smaller aroma compounds with bacterial cells	1566:1628	Among different size fractions of aerosol, the composite system was more effective in finer size fractions (<0.25 μm) of aerosol due to the interaction of smaller aroma compounds with bacterial cells.
34049138	3	6	theme	culturable	560:569	arg1	bacteria					571:578	culturable bacteria	560:578	culturable bacteria of the indoor air	560:596	The evaluation of the inhibitory effect of the composite system on culturable bacteria of the indoor air was done at different sites (air volume from 30 m3 to 80 m3) and in different size fractions of aerosol (<0.25 μm-2.5 μm).
34049138	9	7	theme	bacterial	1614:1622	arg1	cells					1624:1628	bacterial cells	1614:1628	bacterial cells	1614:1628	Among different size fractions of aerosol, the composite system was more effective in finer size fractions (<0.25 μm) of aerosol due to the interaction of smaller aroma compounds with bacterial cells.
34049138	3	8	theme	aerosol	694:700	arg1	fractions					681:689	different size fractions	666:689	different size fractions of aerosol (<0.25 μm-2.5 μm)	666:718	The evaluation of the inhibitory effect of the composite system on culturable bacteria of the indoor air was done at different sites (air volume from 30 m3 to 80 m3) and in different size fractions of aerosol (<0.25 μm-2.5 μm).
34049138	3	9	theme	system	550:555	arg1	effect					526:531	the inhibitory effect	511:531	the inhibitory effect of the composite system on culturable bacteria of the indoor air	511:596	The evaluation of the inhibitory effect of the composite system on culturable bacteria of the indoor air was done at different sites (air volume from 30 m3 to 80 m3) and in different size fractions of aerosol (<0.25 μm-2.5 μm).
34049138	1	10	theme	infectious	193:202	arg1	diseases					204:211	infectious diseases	193:211	infectious diseases	193:211	The bioaerosols present in indoor air play a major role in the transmission of infectious diseases to humans, therefore concern about their exposure is increased recently.
34049138	8	11	from	system	1308:1313	arg1	interaction					1250:1260	the interaction	1246:1260	the interaction of diffused aroma molecule from the composite system with bacterial strains	1246:1336	The results indicate that the interaction of diffused aroma molecule from the composite system with bacterial strains enhanced the production of ROS, resulting in loss of membrane integrity of bacterial cells.
34049138	8	11	from	system	1308:1313	arg1	molecule					1280:1287	diffused aroma molecule	1265:1287	diffused aroma molecule from the composite system	1265:1313	The results indicate that the interaction of diffused aroma molecule from the composite system with bacterial strains enhanced the production of ROS, resulting in loss of membrane integrity of bacterial cells.
34049138	3	12	theme	indoor	587:592	arg1	air					594:596	the indoor air	583:596	the indoor air	583:596	The evaluation of the inhibitory effect of the composite system on culturable bacteria of the indoor air was done at different sites (air volume from 30 m3 to 80 m3) and in different size fractions of aerosol (<0.25 μm-2.5 μm).
34049138	1	13	theme	present	130:136	arg1	bioaerosols					118:128	The bioaerosols	114:128	The bioaerosols present in indoor air	114:150	The bioaerosols present in indoor air play a major role in the transmission of infectious diseases to humans, therefore concern about their exposure is increased recently.
34049138	8	14	theme	integrity	1400:1408	arg1	loss					1383:1386	loss	1383:1386	loss of membrane integrity of bacterial cells	1383:1427	The results indicate that the interaction of diffused aroma molecule from the composite system with bacterial strains enhanced the production of ROS, resulting in loss of membrane integrity of bacterial cells.
34049138	5	15	dep	CFUm-3	898:903	arg1	log					894:896	log	894:896	log	894:896	A significant reduction in culturable bacteria of aerosol (from 6.23 log CFUm-3 to 2.33 log CFUm-3) was observed in presence of cellulose nanofibers and chitosan composites.
34049138	10	16	theme	culturable	1755:1764	arg1	bacteria					1766:1773	the culturable bacteria	1751:1773	the culturable bacteria	1751:1773	The study revealed that LGEO loaded chitosan and cellulose nanofibers composites could be a good option for controlling the culturable bacteria even in small-sized respirable bioaerosol.
34049138	5	17	from	reduction	820:828	arg1	bacteria					844:851	culturable bacteria	833:851	culturable bacteria of aerosol	833:862	A significant reduction in culturable bacteria of aerosol (from 6.23 log CFUm-3 to 2.33 log CFUm-3) was observed in presence of cellulose nanofibers and chitosan composites.
34049138	5	18	theme	log	875:877	arg1	CFUm-3					898:903	6.23 log CFUm-3 to 2.33 log CFUm-3	870:903	6.23 log CFUm-3 to 2.33 log CFUm-3	870:903	A significant reduction in culturable bacteria of aerosol (from 6.23 log CFUm-3 to 2.33 log CFUm-3) was observed in presence of cellulose nanofibers and chitosan composites.
34049138	8	19	theme	bacterial	1413:1421	arg1	cells					1423:1427	bacterial cells	1413:1427	bacterial cells	1413:1427	The results indicate that the interaction of diffused aroma molecule from the composite system with bacterial strains enhanced the production of ROS, resulting in loss of membrane integrity of bacterial cells.
34049138	5	20	dep	2.33	889:892	arg1	to					886:887	to	886:887	to	886:887	A significant reduction in culturable bacteria of aerosol (from 6.23 log CFUm-3 to 2.33 log CFUm-3) was observed in presence of cellulose nanofibers and chitosan composites.
34049138	4	21	contain	had	742:744	arg2	%					782:782	88-91%	777:782	88-91%	777:782	The composite system had high encapsulation efficiency (88-91%) and citrals content.
34049138	4	21	contain	had	742:744	arg2	content					797:803	citrals content	789:803	citrals content	789:803	The composite system had high encapsulation efficiency (88-91%) and citrals content.
34049138	4	21	contain	had	742:744	arg2	efficiency					765:774	high encapsulation efficiency	746:774	high encapsulation efficiency (88-91%)	746:783	The composite system had high encapsulation efficiency (88-91%) and citrals content.
34049138	4	21	contain	had	742:744	arg1	system					735:740	The composite system	721:740	The composite system	721:740	The composite system had high encapsulation efficiency (88-91%) and citrals content.
34049138	2	22	theme	air	477:479	arg1	bioaerosol					481:490	the indoor air bioaerosol	466:490	the indoor air bioaerosol	466:490	In this regard, the present investigation described the preparation of lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF) for controlling the indoor air bioaerosol.
34049138	8	23	theme	diffused	1265:1272	arg1	molecule					1280:1287	diffused aroma molecule	1265:1287	diffused aroma molecule from the composite system	1265:1313	The results indicate that the interaction of diffused aroma molecule from the composite system with bacterial strains enhanced the production of ROS, resulting in loss of membrane integrity of bacterial cells.
34049138	3	24	from	effect	526:531	arg1	bacteria					571:578	culturable bacteria	560:578	culturable bacteria of the indoor air	560:596	The evaluation of the inhibitory effect of the composite system on culturable bacteria of the indoor air was done at different sites (air volume from 30 m3 to 80 m3) and in different size fractions of aerosol (<0.25 μm-2.5 μm).
34049138	1	25	theme	major	159:163	arg1	role					165:168	a major role	157:168	a major role	157:168	The bioaerosols present in indoor air play a major role in the transmission of infectious diseases to humans, therefore concern about their exposure is increased recently.
34049138	8	26	theme	molecule	1280:1287	arg1	interaction					1250:1260	the interaction	1246:1260	the interaction of diffused aroma molecule from the composite system with bacterial strains	1246:1336	The results indicate that the interaction of diffused aroma molecule from the composite system with bacterial strains enhanced the production of ROS, resulting in loss of membrane integrity of bacterial cells.
34049138	0	27	theme	indoor	102:107	arg1	air					109:111	indoor air	102:111	indoor air	102:111	The incorporation of lemongrass oil into chitosan-nanocellulose composite for bioaerosol reduction in indoor air.
34049138	1	28	from	air	148:150	arg1	present					130:136	present	130:136	present	130:136	The bioaerosols present in indoor air play a major role in the transmission of infectious diseases to humans, therefore concern about their exposure is increased recently.
34049138	9	29	theme	composite	1477:1485	arg1	system					1487:1492	the composite system	1473:1492	the composite system	1473:1492	Among different size fractions of aerosol, the composite system was more effective in finer size fractions (<0.25 μm) of aerosol due to the interaction of smaller aroma compounds with bacterial cells.
34049138	9	29	theme	composite	1477:1485	arg1	effective					1503:1511	effective	1503:1511	effective	1503:1511	Among different size fractions of aerosol, the composite system was more effective in finer size fractions (<0.25 μm) of aerosol due to the interaction of smaller aroma compounds with bacterial cells.
34049138	9	30	theme	size	1446:1449	arg1	fractions					1451:1459	different size fractions	1436:1459	different size fractions of aerosol	1436:1470	Among different size fractions of aerosol, the composite system was more effective in finer size fractions (<0.25 μm) of aerosol due to the interaction of smaller aroma compounds with bacterial cells.
34049138	7	31	from	Cf0-3	1118:1122	arg1	bioaerosols					1127:1137	bioaerosols	1127:1137	bioaerosols	1127:1137	Cf0-3 in bioaerosols were inhibited dominantly due to the diffusion of aroma molecules in indoor air.
34049138	3	32	dep	80 m3	652:656	arg1	to					649:650	to	649:650	to	649:650	The evaluation of the inhibitory effect of the composite system on culturable bacteria of the indoor air was done at different sites (air volume from 30 m3 to 80 m3) and in different size fractions of aerosol (<0.25 μm-2.5 μm).
34049138	8	33	from	interaction	1250:1260	arg1	system					1308:1313	the composite system	1294:1313	the composite system	1294:1313	The results indicate that the interaction of diffused aroma molecule from the composite system with bacterial strains enhanced the production of ROS, resulting in loss of membrane integrity of bacterial cells.
34049138	9	34	theme	smaller	1585:1591	arg1	compounds					1599:1607	smaller aroma compounds	1585:1607	smaller aroma compounds	1585:1607	Among different size fractions of aerosol, the composite system was more effective in finer size fractions (<0.25 μm) of aerosol due to the interaction of smaller aroma compounds with bacterial cells.
34049138	10	35	theme	small-sized	1783:1793	arg1	bioaerosol					1806:1815	small-sized respirable bioaerosol	1783:1815	small-sized respirable bioaerosol	1783:1815	The study revealed that LGEO loaded chitosan and cellulose nanofibers composites could be a good option for controlling the culturable bacteria even in small-sized respirable bioaerosol.
34049138	2	36	theme	composites	430:439	arg1	preparation					342:352	the preparation	338:352	the preparation of lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF) for controlling the indoor air bioaerosol	338:490	In this regard, the present investigation described the preparation of lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF) for controlling the indoor air bioaerosol.
34049138	8	37	theme	bacterial	1320:1328	arg1	strains					1330:1336	bacterial strains	1320:1336	bacterial strains	1320:1336	The results indicate that the interaction of diffused aroma molecule from the composite system with bacterial strains enhanced the production of ROS, resulting in loss of membrane integrity of bacterial cells.
34049138	5	38	theme	nanofibers	944:953	arg1	presence					922:929	presence	922:929	presence of cellulose nanofibers and chitosan composites	922:977	A significant reduction in culturable bacteria of aerosol (from 6.23 log CFUm-3 to 2.33 log CFUm-3) was observed in presence of cellulose nanofibers and chitosan composites.
34049138	2	39	theme	cellulose	409:417	arg1	nanofibers					419:428	cellulose nanofibers	409:428	cellulose nanofibers	409:428	In this regard, the present investigation described the preparation of lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF) for controlling the indoor air bioaerosol.
34049138	5	40	located	observed	910:917	arg2	reduction					820:828	A significant reduction	806:828	A significant reduction in culturable bacteria of aerosol (from 6.23 log CFUm-3 to 2.33 log CFUm-3)	806:904	A significant reduction in culturable bacteria of aerosol (from 6.23 log CFUm-3 to 2.33 log CFUm-3) was observed in presence of cellulose nanofibers and chitosan composites.
34049138	5	40	located	observed	910:917	arg1	presence					922:929	presence	922:929	presence of cellulose nanofibers and chitosan composites	922:977	A significant reduction in culturable bacteria of aerosol (from 6.23 log CFUm-3 to 2.33 log CFUm-3) was observed in presence of cellulose nanofibers and chitosan composites.
34049138	10	41	theme	chitosan	1667:1674	arg1	option					1728:1733	a good option	1721:1733	a good option for controlling the culturable bacteria even in small-sized respirable bioaerosol	1721:1815	The study revealed that LGEO loaded chitosan and cellulose nanofibers composites could be a good option for controlling the culturable bacteria even in small-sized respirable bioaerosol.
34049138	10	41	theme	chitosan	1667:1674	arg1	composites					1701:1710	LGEO loaded chitosan and cellulose nanofibers composites	1655:1710	LGEO loaded chitosan and cellulose nanofibers composites	1655:1710	The study revealed that LGEO loaded chitosan and cellulose nanofibers composites could be a good option for controlling the culturable bacteria even in small-sized respirable bioaerosol.
34049138	9	42	theme	finer	1516:1520	arg1	<0.25 μm					1538:1545	<0.25 μm	1538:1545	<0.25 μm	1538:1545	Among different size fractions of aerosol, the composite system was more effective in finer size fractions (<0.25 μm) of aerosol due to the interaction of smaller aroma compounds with bacterial cells.
34049138	9	42	theme	finer	1516:1520	arg1	fractions					1527:1535	finer size fractions	1516:1535	finer size fractions (<0.25 μm) of aerosol	1516:1557	Among different size fractions of aerosol, the composite system was more effective in finer size fractions (<0.25 μm) of aerosol due to the interaction of smaller aroma compounds with bacterial cells.
34049138	6	43	theme	bacterial	984:992	arg1	sp.					1071:1073	Bacillus pseudomycoides sp.	1047:1073	Bacillus pseudomycoides sp.	1047:1073	The bacterial strains such as Staphylococcus sp., Bacillus cereus, Bacillus pseudomycoides sp., Pseudomonas otitidis, and Pseudomonas sp.
34049138	6	43	theme	bacterial	984:992	arg1	sp					1114:1115	Pseudomonas sp	1102:1115	Pseudomonas sp	1102:1115	The bacterial strains such as Staphylococcus sp., Bacillus cereus, Bacillus pseudomycoides sp., Pseudomonas otitidis, and Pseudomonas sp.
34049138	6	43	theme	bacterial	984:992	arg1	strains					994:1000	The bacterial strains	980:1000	The bacterial strains such as Staphylococcus sp., Bacillus cereus, Bacillus pseudomycoides sp., Pseudomonas otitidis, and Pseudomonas sp	980:1115	The bacterial strains such as Staphylococcus sp., Bacillus cereus, Bacillus pseudomycoides sp., Pseudomonas otitidis, and Pseudomonas sp.
34049138	6	43	theme	bacterial	984:992	arg1	cereus					1039:1044	Bacillus cereus	1030:1044	Bacillus cereus	1030:1044	The bacterial strains such as Staphylococcus sp., Bacillus cereus, Bacillus pseudomycoides sp., Pseudomonas otitidis, and Pseudomonas sp.
34049138	6	43	theme	bacterial	984:992	arg1	otitidis					1088:1095	Pseudomonas otitidis	1076:1095	Pseudomonas otitidis	1076:1095	The bacterial strains such as Staphylococcus sp., Bacillus cereus, Bacillus pseudomycoides sp., Pseudomonas otitidis, and Pseudomonas sp.
34049138	6	43	theme	bacterial	984:992	arg1	sp.					1025:1027	Staphylococcus sp.	1010:1027	Staphylococcus sp.	1010:1027	The bacterial strains such as Staphylococcus sp., Bacillus cereus, Bacillus pseudomycoides sp., Pseudomonas otitidis, and Pseudomonas sp.
34049138	3	44	theme	air	627:629	arg1	volume					631:636	air volume	627:636	air volume from 30 m3 to 80 m3	627:656	The evaluation of the inhibitory effect of the composite system on culturable bacteria of the indoor air was done at different sites (air volume from 30 m3 to 80 m3) and in different size fractions of aerosol (<0.25 μm-2.5 μm).
34049138	2	45	theme	chitosan	396:403	arg1	CH/CNF					442:447	CH/CNF	442:447	CH/CNF	442:447	In this regard, the present investigation described the preparation of lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF) for controlling the indoor air bioaerosol.
34049138	2	45	theme	chitosan	396:403	arg1	composites					430:439	lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites	357:439	lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF)	357:448	In this regard, the present investigation described the preparation of lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF) for controlling the indoor air bioaerosol.
34049138	0	46	theme	oil	32:34	arg1	incorporation					4:16	The incorporation	0:16	The incorporation of lemongrass oil into chitosan-nanocellulose	0:62	The incorporation of lemongrass oil into chitosan-nanocellulose composite for bioaerosol reduction in indoor air.
34049138	5	47	theme	composites	968:977	arg1	presence					922:929	presence	922:929	presence of cellulose nanofibers and chitosan composites	922:977	A significant reduction in culturable bacteria of aerosol (from 6.23 log CFUm-3 to 2.33 log CFUm-3) was observed in presence of cellulose nanofibers and chitosan composites.
34049138	8	48	theme	ROS	1365:1367	arg1	production					1351:1360	the production	1347:1360	the production of ROS	1347:1367	The results indicate that the interaction of diffused aroma molecule from the composite system with bacterial strains enhanced the production of ROS, resulting in loss of membrane integrity of bacterial cells.
34049138	10	49	theme	loaded	1660:1665	arg1	option					1728:1733	a good option	1721:1733	a good option for controlling the culturable bacteria even in small-sized respirable bioaerosol	1721:1815	The study revealed that LGEO loaded chitosan and cellulose nanofibers composites could be a good option for controlling the culturable bacteria even in small-sized respirable bioaerosol.
34049138	10	49	theme	loaded	1660:1665	arg1	composites					1701:1710	LGEO loaded chitosan and cellulose nanofibers composites	1655:1710	LGEO loaded chitosan and cellulose nanofibers composites	1655:1710	The study revealed that LGEO loaded chitosan and cellulose nanofibers composites could be a good option for controlling the culturable bacteria even in small-sized respirable bioaerosol.
34049138	7	50	theme	indoor	1208:1213	arg1	air					1215:1217	indoor air	1208:1217	indoor air	1208:1217	Cf0-3 in bioaerosols were inhibited dominantly due to the diffusion of aroma molecules in indoor air.
34049138	2	51	theme	essential	368:376	arg1	LGEO					383:386	LGEO	383:386	LGEO	383:386	In this regard, the present investigation described the preparation of lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF) for controlling the indoor air bioaerosol.
34049138	2	51	theme	essential	368:376	arg1	oil					378:380	lemongrass essential oil	357:380	lemongrass essential oil (LGEO)	357:387	In this regard, the present investigation described the preparation of lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF) for controlling the indoor air bioaerosol.
34049138	5	52	theme	cellulose	934:942	arg1	nanofibers					944:953	cellulose nanofibers	934:953	cellulose nanofibers	934:953	A significant reduction in culturable bacteria of aerosol (from 6.23 log CFUm-3 to 2.33 log CFUm-3) was observed in presence of cellulose nanofibers and chitosan composites.
34049138	7	53	theme	molecules	1195:1203	arg1	diffusion					1176:1184	the diffusion	1172:1184	the diffusion of aroma molecules in indoor air	1172:1217	Cf0-3 in bioaerosols were inhibited dominantly due to the diffusion of aroma molecules in indoor air.
34049138	7	54	from	diffusion	1176:1184	arg1	air					1215:1217	indoor air	1208:1217	indoor air	1208:1217	Cf0-3 in bioaerosols were inhibited dominantly due to the diffusion of aroma molecules in indoor air.
34049138	3	55	from	evaluation	497:506	arg1	bacteria					571:578	culturable bacteria	560:578	culturable bacteria of the indoor air	560:596	The evaluation of the inhibitory effect of the composite system on culturable bacteria of the indoor air was done at different sites (air volume from 30 m3 to 80 m3) and in different size fractions of aerosol (<0.25 μm-2.5 μm).
34049138	10	56	theme	cellulose	1680:1688	arg1	nanofibers					1690:1699	cellulose nanofibers	1680:1699	cellulose nanofibers	1680:1699	The study revealed that LGEO loaded chitosan and cellulose nanofibers composites could be a good option for controlling the culturable bacteria even in small-sized respirable bioaerosol.
34049138	3	57	theme	inhibitory	515:524	arg1	effect					526:531	the inhibitory effect	511:531	the inhibitory effect of the composite system on culturable bacteria of the indoor air	511:596	The evaluation of the inhibitory effect of the composite system on culturable bacteria of the indoor air was done at different sites (air volume from 30 m3 to 80 m3) and in different size fractions of aerosol (<0.25 μm-2.5 μm).
34049138	9	58	theme	aroma	1593:1597	arg1	compounds					1599:1607	smaller aroma compounds	1585:1607	smaller aroma compounds	1585:1607	Among different size fractions of aerosol, the composite system was more effective in finer size fractions (<0.25 μm) of aerosol due to the interaction of smaller aroma compounds with bacterial cells.
34049138	6	59	theme	Pseudomonas	1102:1112	arg1	sp					1114:1115	Pseudomonas sp	1102:1115	Pseudomonas sp	1102:1115	The bacterial strains such as Staphylococcus sp., Bacillus cereus, Bacillus pseudomycoides sp., Pseudomonas otitidis, and Pseudomonas sp.
34049138	3	60	theme	size	676:679	arg1	fractions					681:689	different size fractions	666:689	different size fractions of aerosol (<0.25 μm-2.5 μm)	666:718	The evaluation of the inhibitory effect of the composite system on culturable bacteria of the indoor air was done at different sites (air volume from 30 m3 to 80 m3) and in different size fractions of aerosol (<0.25 μm-2.5 μm).
34049138	3	61	theme	composite	540:548	arg1	system					550:555	the composite system	536:555	the composite system	536:555	The evaluation of the inhibitory effect of the composite system on culturable bacteria of the indoor air was done at different sites (air volume from 30 m3 to 80 m3) and in different size fractions of aerosol (<0.25 μm-2.5 μm).
34049138	5	62	theme	aerosol	856:862	arg1	bacteria					844:851	culturable bacteria	833:851	culturable bacteria of aerosol	833:862	A significant reduction in culturable bacteria of aerosol (from 6.23 log CFUm-3 to 2.33 log CFUm-3) was observed in presence of cellulose nanofibers and chitosan composites.
34049138	3	63	from	80 m3	652:656	arg1	volume					631:636	air volume	627:636	air volume from 30 m3 to 80 m3	627:656	The evaluation of the inhibitory effect of the composite system on culturable bacteria of the indoor air was done at different sites (air volume from 30 m3 to 80 m3) and in different size fractions of aerosol (<0.25 μm-2.5 μm).
34049138	1	64	theme	diseases	204:211	arg1	transmission					177:188	the transmission	173:188	the transmission of infectious diseases to humans	173:221	The bioaerosols present in indoor air play a major role in the transmission of infectious diseases to humans, therefore concern about their exposure is increased recently.
34049138	8	65	theme	membrane	1391:1398	arg1	integrity					1400:1408	membrane integrity	1391:1408	membrane integrity of bacterial cells	1391:1427	The results indicate that the interaction of diffused aroma molecule from the composite system with bacterial strains enhanced the production of ROS, resulting in loss of membrane integrity of bacterial cells.
34049138	1	66	theme	indoor	141:146	arg1	air					148:150	indoor air	141:150	indoor air	141:150	The bioaerosols present in indoor air play a major role in the transmission of infectious diseases to humans, therefore concern about their exposure is increased recently.
34049138	10	67	theme	good	1723:1726	arg1	option					1728:1733	a good option	1721:1733	a good option for controlling the culturable bacteria even in small-sized respirable bioaerosol	1721:1815	The study revealed that LGEO loaded chitosan and cellulose nanofibers composites could be a good option for controlling the culturable bacteria even in small-sized respirable bioaerosol.
34049138	10	67	theme	good	1723:1726	arg1	composites					1701:1710	LGEO loaded chitosan and cellulose nanofibers composites	1655:1710	LGEO loaded chitosan and cellulose nanofibers composites	1655:1710	The study revealed that LGEO loaded chitosan and cellulose nanofibers composites could be a good option for controlling the culturable bacteria even in small-sized respirable bioaerosol.
34049138	4	68	theme	high	746:749	arg1	efficiency					765:774	high encapsulation efficiency	746:774	high encapsulation efficiency (88-91%)	746:783	The composite system had high encapsulation efficiency (88-91%) and citrals content.
34049138	4	68	theme	high	746:749	arg1	%					782:782	88-91%	777:782	88-91%	777:782	The composite system had high encapsulation efficiency (88-91%) and citrals content.
34049138	9	69	theme	different	1436:1444	arg1	fractions					1451:1459	different size fractions	1436:1459	different size fractions of aerosol	1436:1470	Among different size fractions of aerosol, the composite system was more effective in finer size fractions (<0.25 μm) of aerosol due to the interaction of smaller aroma compounds with bacterial cells.
34049138	8	70	theme	cells	1423:1427	arg1	integrity					1400:1408	membrane integrity	1391:1408	membrane integrity of bacterial cells	1391:1427	The results indicate that the interaction of diffused aroma molecule from the composite system with bacterial strains enhanced the production of ROS, resulting in loss of membrane integrity of bacterial cells.
34049138	0	71	theme	bioaerosol	78:87	arg1	reduction					89:97	bioaerosol reduction	78:97	bioaerosol reduction in indoor air	78:111	The incorporation of lemongrass oil into chitosan-nanocellulose composite for bioaerosol reduction in indoor air.
34049138	3	72	theme	air	594:596	arg1	bacteria					571:578	culturable bacteria	560:578	culturable bacteria of the indoor air	560:596	The evaluation of the inhibitory effect of the composite system on culturable bacteria of the indoor air was done at different sites (air volume from 30 m3 to 80 m3) and in different size fractions of aerosol (<0.25 μm-2.5 μm).
34049138	5	73	theme	culturable	833:842	arg1	bacteria					844:851	culturable bacteria	833:851	culturable bacteria of aerosol	833:862	A significant reduction in culturable bacteria of aerosol (from 6.23 log CFUm-3 to 2.33 log CFUm-3) was observed in presence of cellulose nanofibers and chitosan composites.
34049138	9	74	from	effective	1503:1511	arg1	<0.25 μm					1538:1545	<0.25 μm	1538:1545	<0.25 μm	1538:1545	Among different size fractions of aerosol, the composite system was more effective in finer size fractions (<0.25 μm) of aerosol due to the interaction of smaller aroma compounds with bacterial cells.
34049138	9	74	from	effective	1503:1511	arg1	fractions					1527:1535	finer size fractions	1516:1535	finer size fractions (<0.25 μm) of aerosol	1516:1557	Among different size fractions of aerosol, the composite system was more effective in finer size fractions (<0.25 μm) of aerosol due to the interaction of smaller aroma compounds with bacterial cells.
34049138	5	75	dep	log	894:896	arg1	2.33					889:892	2.33	889:892	2.33	889:892	A significant reduction in culturable bacteria of aerosol (from 6.23 log CFUm-3 to 2.33 log CFUm-3) was observed in presence of cellulose nanofibers and chitosan composites.
34049138	9	76	theme	aerosol	1464:1470	arg1	fractions					1451:1459	different size fractions	1436:1459	different size fractions of aerosol	1436:1470	Among different size fractions of aerosol, the composite system was more effective in finer size fractions (<0.25 μm) of aerosol due to the interaction of smaller aroma compounds with bacterial cells.
34049138	2	77	theme	indoor	470:475	arg1	bioaerosol					481:490	the indoor air bioaerosol	466:490	the indoor air bioaerosol	466:490	In this regard, the present investigation described the preparation of lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF) for controlling the indoor air bioaerosol.
34049138	10	78	theme	nanofibers	1690:1699	arg1	option					1728:1733	a good option	1721:1733	a good option for controlling the culturable bacteria even in small-sized respirable bioaerosol	1721:1815	The study revealed that LGEO loaded chitosan and cellulose nanofibers composites could be a good option for controlling the culturable bacteria even in small-sized respirable bioaerosol.
34049138	10	78	theme	nanofibers	1690:1699	arg1	composites					1701:1710	LGEO loaded chitosan and cellulose nanofibers composites	1655:1710	LGEO loaded chitosan and cellulose nanofibers composites	1655:1710	The study revealed that LGEO loaded chitosan and cellulose nanofibers composites could be a good option for controlling the culturable bacteria even in small-sized respirable bioaerosol.
34049138	8	79	theme	aroma	1274:1278	arg1	molecule					1280:1287	diffused aroma molecule	1265:1287	diffused aroma molecule from the composite system	1265:1313	The results indicate that the interaction of diffused aroma molecule from the composite system with bacterial strains enhanced the production of ROS, resulting in loss of membrane integrity of bacterial cells.
34049138	4	80	theme	composite	725:733	arg1	system					735:740	The composite system	721:740	The composite system	721:740	The composite system had high encapsulation efficiency (88-91%) and citrals content.
34049138	8	81	theme	composite	1298:1306	arg1	system					1308:1313	the composite system	1294:1313	the composite system	1294:1313	The results indicate that the interaction of diffused aroma molecule from the composite system with bacterial strains enhanced the production of ROS, resulting in loss of membrane integrity of bacterial cells.
34049138	3	82	from	bacteria	571:578	arg1	evaluation					497:506	The evaluation	493:506	The evaluation of the inhibitory effect of the composite system on culturable bacteria of the indoor air	493:596	The evaluation of the inhibitory effect of the composite system on culturable bacteria of the indoor air was done at different sites (air volume from 30 m3 to 80 m3) and in different size fractions of aerosol (<0.25 μm-2.5 μm).
34049138	0	83	from	reduction	89:97	arg1	air					109:111	indoor air	102:111	indoor air	102:111	The incorporation of lemongrass oil into chitosan-nanocellulose composite for bioaerosol reduction in indoor air.
34049138	0	84	theme	lemongrass	21:30	arg1	oil					32:34	lemongrass oil	21:34	lemongrass oil	21:34	The incorporation of lemongrass oil into chitosan-nanocellulose composite for bioaerosol reduction in indoor air.
34049138	1	85	from	present	130:136	arg1	air					148:150	indoor air	141:150	indoor air	141:150	The bioaerosols present in indoor air play a major role in the transmission of infectious diseases to humans, therefore concern about their exposure is increased recently.
34049138	2	86	theme	nanofibers	419:428	arg1	CH/CNF					442:447	CH/CNF	442:447	CH/CNF	442:447	In this regard, the present investigation described the preparation of lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF) for controlling the indoor air bioaerosol.
34049138	2	86	theme	nanofibers	419:428	arg1	composites					430:439	lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites	357:439	lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF)	357:448	In this regard, the present investigation described the preparation of lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF) for controlling the indoor air bioaerosol.
34049138	10	87	theme	respirable	1795:1804	arg1	bioaerosol					1806:1815	small-sized respirable bioaerosol	1783:1815	small-sized respirable bioaerosol	1783:1815	The study revealed that LGEO loaded chitosan and cellulose nanofibers composites could be a good option for controlling the culturable bacteria even in small-sized respirable bioaerosol.
34049138	1	88	dep	play	152:155	arg1	concern					234:240	concern	234:240	concern about their exposure is increased recently	234:283	The bioaerosols present in indoor air play a major role in the transmission of infectious diseases to humans, therefore concern about their exposure is increased recently.
34049138	9	89	with	interaction	1570:1580	arg1	cells					1624:1628	bacterial cells	1614:1628	bacterial cells	1614:1628	Among different size fractions of aerosol, the composite system was more effective in finer size fractions (<0.25 μm) of aerosol due to the interaction of smaller aroma compounds with bacterial cells.
34049138	9	90	theme	size	1522:1525	arg1	<0.25 μm					1538:1545	<0.25 μm	1538:1545	<0.25 μm	1538:1545	Among different size fractions of aerosol, the composite system was more effective in finer size fractions (<0.25 μm) of aerosol due to the interaction of smaller aroma compounds with bacterial cells.
34049138	9	90	theme	size	1522:1525	arg1	fractions					1527:1535	finer size fractions	1516:1535	finer size fractions (<0.25 μm) of aerosol	1516:1557	Among different size fractions of aerosol, the composite system was more effective in finer size fractions (<0.25 μm) of aerosol due to the interaction of smaller aroma compounds with bacterial cells.
34049138	9	91	from	fractions	1527:1535	arg1	system					1487:1492	the composite system	1473:1492	the composite system	1473:1492	Among different size fractions of aerosol, the composite system was more effective in finer size fractions (<0.25 μm) of aerosol due to the interaction of smaller aroma compounds with bacterial cells.
34049138	9	91	from	fractions	1527:1535	arg1	effective					1503:1511	effective	1503:1511	effective	1503:1511	Among different size fractions of aerosol, the composite system was more effective in finer size fractions (<0.25 μm) of aerosol due to the interaction of smaller aroma compounds with bacterial cells.
34049138	3	92	theme	different	610:618	arg1	sites					620:624	different sites	610:624	different sites (air volume from 30 m3 to 80 m3)	610:657	The evaluation of the inhibitory effect of the composite system on culturable bacteria of the indoor air was done at different sites (air volume from 30 m3 to 80 m3) and in different size fractions of aerosol (<0.25 μm-2.5 μm).
34049138	2	93	dep	loaded	389:394	arg1	LGEO					383:386	LGEO	383:386	LGEO	383:386	In this regard, the present investigation described the preparation of lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF) for controlling the indoor air bioaerosol.
34049138	2	93	dep	loaded	389:394	arg1	oil					378:380	lemongrass essential oil	357:380	lemongrass essential oil (LGEO)	357:387	In this regard, the present investigation described the preparation of lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF) for controlling the indoor air bioaerosol.
34049138	2	94	theme	loaded	389:394	arg1	CH/CNF					442:447	CH/CNF	442:447	CH/CNF	442:447	In this regard, the present investigation described the preparation of lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF) for controlling the indoor air bioaerosol.
34049138	2	94	theme	loaded	389:394	arg1	composites					430:439	lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites	357:439	lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF)	357:448	In this regard, the present investigation described the preparation of lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF) for controlling the indoor air bioaerosol.
34049138	5	95	theme	chitosan	959:966	arg1	composites					968:977	chitosan composites	959:977	chitosan composites	959:977	A significant reduction in culturable bacteria of aerosol (from 6.23 log CFUm-3 to 2.33 log CFUm-3) was observed in presence of cellulose nanofibers and chitosan composites.
34049138	8	96	with	interaction	1250:1260	arg1	strains					1330:1336	bacterial strains	1320:1336	bacterial strains	1320:1336	The results indicate that the interaction of diffused aroma molecule from the composite system with bacterial strains enhanced the production of ROS, resulting in loss of membrane integrity of bacterial cells.
34049138	2	97	theme	present	306:312	arg1	investigation					314:326	the present investigation	302:326	the present investigation	302:326	In this regard, the present investigation described the preparation of lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF) for controlling the indoor air bioaerosol.
34049138	3	98	dep	sites	620:624	arg1	volume					631:636	air volume	627:636	air volume from 30 m3 to 80 m3	627:656	The evaluation of the inhibitory effect of the composite system on culturable bacteria of the indoor air was done at different sites (air volume from 30 m3 to 80 m3) and in different size fractions of aerosol (<0.25 μm-2.5 μm).
34049138	9	99	theme	aerosol	1551:1557	arg1	<0.25 μm					1538:1545	<0.25 μm	1538:1545	<0.25 μm	1538:1545	Among different size fractions of aerosol, the composite system was more effective in finer size fractions (<0.25 μm) of aerosol due to the interaction of smaller aroma compounds with bacterial cells.
34049138	9	99	theme	aerosol	1551:1557	arg1	fractions					1527:1535	finer size fractions	1516:1535	finer size fractions (<0.25 μm) of aerosol	1516:1557	Among different size fractions of aerosol, the composite system was more effective in finer size fractions (<0.25 μm) of aerosol due to the interaction of smaller aroma compounds with bacterial cells.
34049138	7	100	theme	aroma	1189:1193	arg1	molecules					1195:1203	aroma molecules	1189:1203	aroma molecules	1189:1203	Cf0-3 in bioaerosols were inhibited dominantly due to the diffusion of aroma molecules in indoor air.
34049138	1	101	attach	present	130:136	arg2	bioaerosols					118:128	The bioaerosols	114:128	The bioaerosols present in indoor air	114:150	The bioaerosols present in indoor air play a major role in the transmission of infectious diseases to humans, therefore concern about their exposure is increased recently.
34049138	1	101	attach	present	130:136	arg1	air					148:150	indoor air	141:150	indoor air	141:150	The bioaerosols present in indoor air play a major role in the transmission of infectious diseases to humans, therefore concern about their exposure is increased recently.
34049138	4	102	theme	encapsulation	751:763	arg1	efficiency					765:774	high encapsulation efficiency	746:774	high encapsulation efficiency (88-91%)	746:783	The composite system had high encapsulation efficiency (88-91%) and citrals content.
34049138	4	102	theme	encapsulation	751:763	arg1	%					782:782	88-91%	777:782	88-91%	777:782	The composite system had high encapsulation efficiency (88-91%) and citrals content.
34049138	2	103	theme	lemongrass	357:366	arg1	LGEO					383:386	LGEO	383:386	LGEO	383:386	In this regard, the present investigation described the preparation of lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF) for controlling the indoor air bioaerosol.
34049138	2	103	theme	lemongrass	357:366	arg1	oil					378:380	lemongrass essential oil	357:380	lemongrass essential oil (LGEO)	357:387	In this regard, the present investigation described the preparation of lemongrass essential oil (LGEO) loaded chitosan and cellulose nanofibers composites (CH/CNF) for controlling the indoor air bioaerosol.
32431565	7	0	theme	computational	1328:1340	arg1	results					1342:1348	computational results	1328:1348	computational results	1328:1348	Based on experimental data and computational results, a revised mechanism for this β-mannosylation is proposed.
32431565	7	1	theme	revised	1353:1359	arg1	mechanism					1361:1369	a revised mechanism	1351:1369	a revised mechanism for this β-mannosylation	1351:1394	Based on experimental data and computational results, a revised mechanism for this β-mannosylation is proposed.
32431565	5	2	theme	progress	852:859	arg1	analysis					869:876	Reaction progress kinetic analysis	843:876	Reaction progress kinetic analysis	843:876	Reaction progress kinetic analysis suggested that monomeric cesium alkoxides be the key reactive species for alkylation with electrophiles.
32431565	1	3	theme	bicyclic	292:299	arg1	D-mannoses					301:310	conformationally restricted bicyclic D-mannoses	264:310	conformationally restricted bicyclic D-mannoses	264:310	A number of structurally diverse D-mannose-derived lactols, including various deoxy-D-mannoses and conformationally restricted bicyclic D-mannoses, have been synthesized and investigated in mechanistic studies of β-mannosylation via Cs2CO3-mediated anomeric O-alkylation.
32431565	2	4	theme	parent	564:569	arg1	mannose					571:577	their corresponding parent mannose	544:577	their corresponding parent mannose	544:577	It was found that deoxy mannoses or conformationally restricted bicyclic D-mannoses are not as reactive as their corresponding parent mannose.
32431565	1	5	theme	Cs2CO3-mediated	398:412	arg1	O-alkylation					423:434	Cs2CO3-mediated anomeric O-alkylation	398:434	Cs2CO3-mediated anomeric O-alkylation	398:434	A number of structurally diverse D-mannose-derived lactols, including various deoxy-D-mannoses and conformationally restricted bicyclic D-mannoses, have been synthesized and investigated in mechanistic studies of β-mannosylation via Cs2CO3-mediated anomeric O-alkylation.
32431565	5	6	theme	cesium	903:908	arg1	alkoxides					910:918	monomeric cesium alkoxides	893:918	monomeric cesium alkoxides	893:918	Reaction progress kinetic analysis suggested that monomeric cesium alkoxides be the key reactive species for alkylation with electrophiles.
32431565	5	6	theme	cesium	903:908	arg1	species					940:946	the key reactive species	923:946	the key reactive species for alkylation with electrophiles	923:980	Reaction progress kinetic analysis suggested that monomeric cesium alkoxides be the key reactive species for alkylation with electrophiles.
32431565	6	7	theme	hydroxyl	1099:1106	arg1	group					1108:1112	the anomeric hydroxyl group	1086:1112	the anomeric hydroxyl group	1086:1112	DFT calculations supported that oxygen atoms at C2, C3, and C6 of mannose promote the deprotonation of the anomeric hydroxyl group by Cs2CO3 and chelating interactions between Cs and these oxygen atoms favour the formation of equatorial anomeric alkoxides, leading to the highly β-selective anomeric O-alkylation.
32431565	1	8	theme	anomeric	414:421	arg1	O-alkylation					423:434	Cs2CO3-mediated anomeric O-alkylation	398:434	Cs2CO3-mediated anomeric O-alkylation	398:434	A number of structurally diverse D-mannose-derived lactols, including various deoxy-D-mannoses and conformationally restricted bicyclic D-mannoses, have been synthesized and investigated in mechanistic studies of β-mannosylation via Cs2CO3-mediated anomeric O-alkylation.
32431565	2	9	theme	corresponding	550:562	arg1	mannose					571:577	their corresponding parent mannose	544:577	their corresponding parent mannose	544:577	It was found that deoxy mannoses or conformationally restricted bicyclic D-mannoses are not as reactive as their corresponding parent mannose.
32431565	8	10	theme	efficient	1472:1480	arg1	synthesis					1482:1490	an efficient synthesis	1469:1490	an efficient synthesis of the hexasaccharide core of complex fucosylated N-linked glycans	1469:1557	The utilization of this β-mannosylation was demonstrated by an efficient synthesis of the hexasaccharide core of complex fucosylated N-linked glycans.
32431565	6	11	theme	anomeric	1090:1097	arg1	group					1108:1112	the anomeric hydroxyl group	1086:1112	the anomeric hydroxyl group	1086:1112	DFT calculations supported that oxygen atoms at C2, C3, and C6 of mannose promote the deprotonation of the anomeric hydroxyl group by Cs2CO3 and chelating interactions between Cs and these oxygen atoms favour the formation of equatorial anomeric alkoxides, leading to the highly β-selective anomeric O-alkylation.
32431565	7	12	theme	experimental	1306:1317	arg1	data					1319:1322	experimental data	1306:1322	experimental data	1306:1322	Based on experimental data and computational results, a revised mechanism for this β-mannosylation is proposed.
32431565	6	13	theme	anomeric	1220:1227	arg1	alkoxides					1229:1237	equatorial anomeric alkoxides	1209:1237	equatorial anomeric alkoxides	1209:1237	DFT calculations supported that oxygen atoms at C2, C3, and C6 of mannose promote the deprotonation of the anomeric hydroxyl group by Cs2CO3 and chelating interactions between Cs and these oxygen atoms favour the formation of equatorial anomeric alkoxides, leading to the highly β-selective anomeric O-alkylation.
32431565	0	14	theme	Core	119:122	arg1	Synthesis					87:95	Synthesis	87:95	Synthesis	87:95	β-Mannosylation via O-Alkylation of Anomeric Cesium Alkoxides: Mechanistic Studies and Synthesis of the Hexasaccharide Core of Complex Fucosylated N-Linked Glycans.
32431565	0	14	theme	Core	119:122	arg1	Studies					75:81	Mechanistic Studies	63:81	Mechanistic Studies	63:81	β-Mannosylation via O-Alkylation of Anomeric Cesium Alkoxides: Mechanistic Studies and Synthesis of the Hexasaccharide Core of Complex Fucosylated N-Linked Glycans.
32431565	6	15	theme	chelating	1128:1136	arg1	interactions					1138:1149	chelating interactions	1128:1149	chelating interactions between Cs	1128:1160	DFT calculations supported that oxygen atoms at C2, C3, and C6 of mannose promote the deprotonation of the anomeric hydroxyl group by Cs2CO3 and chelating interactions between Cs and these oxygen atoms favour the formation of equatorial anomeric alkoxides, leading to the highly β-selective anomeric O-alkylation.
32431565	4	16	theme	alkoxides	758:766	arg1	studies					713:719	NMR studies	709:719	NMR studies of D-mannose-derived anomeric cesium alkoxides	709:766	NMR studies of D-mannose-derived anomeric cesium alkoxides indicated the predominance of the equatorial β-anomer after deprotonation.
32431565	4	17	theme	anomeric	742:749	arg1	alkoxides					758:766	D-mannose-derived anomeric cesium alkoxides	724:766	D-mannose-derived anomeric cesium alkoxides	724:766	NMR studies of D-mannose-derived anomeric cesium alkoxides indicated the predominance of the equatorial β-anomer after deprotonation.
32431565	0	18	theme	Hexasaccharide	104:117	arg1	Core					119:122	the Hexasaccharide Core	100:122	the Hexasaccharide Core of Complex Fucosylated N-Linked Glycans	100:162	β-Mannosylation via O-Alkylation of Anomeric Cesium Alkoxides: Mechanistic Studies and Synthesis of the Hexasaccharide Core of Complex Fucosylated N-Linked Glycans.
32431565	6	19	theme	oxygen	1015:1020	arg1	atoms					1022:1026	oxygen atoms	1015:1026	oxygen atoms at C2, C3, and C6 of mannose	1015:1055	DFT calculations supported that oxygen atoms at C2, C3, and C6 of mannose promote the deprotonation of the anomeric hydroxyl group by Cs2CO3 and chelating interactions between Cs and these oxygen atoms favour the formation of equatorial anomeric alkoxides, leading to the highly β-selective anomeric O-alkylation.
32431565	1	20	link	D-mannose-derived	198:214	arg1	D-mannoses					301:310	conformationally restricted bicyclic D-mannoses	264:310	conformationally restricted bicyclic D-mannoses	264:310	A number of structurally diverse D-mannose-derived lactols, including various deoxy-D-mannoses and conformationally restricted bicyclic D-mannoses, have been synthesized and investigated in mechanistic studies of β-mannosylation via Cs2CO3-mediated anomeric O-alkylation.
32431565	1	20	link	D-mannose-derived	198:214	arg1	deoxy-D-mannoses					243:258	various deoxy-D-mannoses	235:258	various deoxy-D-mannoses	235:258	A number of structurally diverse D-mannose-derived lactols, including various deoxy-D-mannoses and conformationally restricted bicyclic D-mannoses, have been synthesized and investigated in mechanistic studies of β-mannosylation via Cs2CO3-mediated anomeric O-alkylation.
32431565	1	20	link	D-mannose-derived	198:214	arg1	lactols					216:222	structurally diverse D-mannose-derived lactols	177:222	structurally diverse D-mannose-derived lactols	177:222	A number of structurally diverse D-mannose-derived lactols, including various deoxy-D-mannoses and conformationally restricted bicyclic D-mannoses, have been synthesized and investigated in mechanistic studies of β-mannosylation via Cs2CO3-mediated anomeric O-alkylation.
32431565	4	21	theme	NMR	709:711	arg1	studies					713:719	NMR studies	709:719	NMR studies of D-mannose-derived anomeric cesium alkoxides	709:766	NMR studies of D-mannose-derived anomeric cesium alkoxides indicated the predominance of the equatorial β-anomer after deprotonation.
32431565	6	22	theme	anomeric	1274:1281	arg1	O-alkylation					1283:1294	the highly β-selective anomeric O-alkylation	1251:1294	the highly β-selective anomeric O-alkylation	1251:1294	DFT calculations supported that oxygen atoms at C2, C3, and C6 of mannose promote the deprotonation of the anomeric hydroxyl group by Cs2CO3 and chelating interactions between Cs and these oxygen atoms favour the formation of equatorial anomeric alkoxides, leading to the highly β-selective anomeric O-alkylation.
32431565	2	23	theme	deoxy	455:459	arg1	mannoses					461:468	deoxy mannoses	455:468	deoxy mannoses	455:468	It was found that deoxy mannoses or conformationally restricted bicyclic D-mannoses are not as reactive as their corresponding parent mannose.
32431565	6	24	from	C3	1035:1036	arg1	atoms					1022:1026	oxygen atoms	1015:1026	oxygen atoms at C2, C3, and C6 of mannose	1015:1055	DFT calculations supported that oxygen atoms at C2, C3, and C6 of mannose promote the deprotonation of the anomeric hydroxyl group by Cs2CO3 and chelating interactions between Cs and these oxygen atoms favour the formation of equatorial anomeric alkoxides, leading to the highly β-selective anomeric O-alkylation.
32431565	6	25	theme	β-selective	1262:1272	arg1	O-alkylation					1283:1294	the highly β-selective anomeric O-alkylation	1251:1294	the highly β-selective anomeric O-alkylation	1251:1294	DFT calculations supported that oxygen atoms at C2, C3, and C6 of mannose promote the deprotonation of the anomeric hydroxyl group by Cs2CO3 and chelating interactions between Cs and these oxygen atoms favour the formation of equatorial anomeric alkoxides, leading to the highly β-selective anomeric O-alkylation.
32431565	6	26	theme	oxygen	1172:1177	arg1	atoms					1179:1183	these oxygen atoms	1166:1183	these oxygen atoms	1166:1183	DFT calculations supported that oxygen atoms at C2, C3, and C6 of mannose promote the deprotonation of the anomeric hydroxyl group by Cs2CO3 and chelating interactions between Cs and these oxygen atoms favour the formation of equatorial anomeric alkoxides, leading to the highly β-selective anomeric O-alkylation.
32431565	1	27	theme	diverse	190:196	arg1	D-mannoses					301:310	conformationally restricted bicyclic D-mannoses	264:310	conformationally restricted bicyclic D-mannoses	264:310	A number of structurally diverse D-mannose-derived lactols, including various deoxy-D-mannoses and conformationally restricted bicyclic D-mannoses, have been synthesized and investigated in mechanistic studies of β-mannosylation via Cs2CO3-mediated anomeric O-alkylation.
32431565	1	27	theme	diverse	190:196	arg1	deoxy-D-mannoses					243:258	various deoxy-D-mannoses	235:258	various deoxy-D-mannoses	235:258	A number of structurally diverse D-mannose-derived lactols, including various deoxy-D-mannoses and conformationally restricted bicyclic D-mannoses, have been synthesized and investigated in mechanistic studies of β-mannosylation via Cs2CO3-mediated anomeric O-alkylation.
32431565	1	27	theme	diverse	190:196	arg1	lactols					216:222	structurally diverse D-mannose-derived lactols	177:222	structurally diverse D-mannose-derived lactols	177:222	A number of structurally diverse D-mannose-derived lactols, including various deoxy-D-mannoses and conformationally restricted bicyclic D-mannoses, have been synthesized and investigated in mechanistic studies of β-mannosylation via Cs2CO3-mediated anomeric O-alkylation.
32431565	5	28	theme	key	927:929	arg1	alkoxides					910:918	monomeric cesium alkoxides	893:918	monomeric cesium alkoxides	893:918	Reaction progress kinetic analysis suggested that monomeric cesium alkoxides be the key reactive species for alkylation with electrophiles.
32431565	5	28	theme	key	927:929	arg1	species					940:946	the key reactive species	923:946	the key reactive species for alkylation with electrophiles	923:980	Reaction progress kinetic analysis suggested that monomeric cesium alkoxides be the key reactive species for alkylation with electrophiles.
32431565	8	29	gly	fucosylated	1530:1540	arg1	glycans					1551:1557	complex fucosylated N-linked glycans	1522:1557	complex fucosylated N-linked glycans	1522:1557	The utilization of this β-mannosylation was demonstrated by an efficient synthesis of the hexasaccharide core of complex fucosylated N-linked glycans.
32431565	6	30	theme	group	1108:1112	arg1	deprotonation					1069:1081	the deprotonation	1065:1081	the deprotonation of the anomeric hydroxyl group	1065:1112	DFT calculations supported that oxygen atoms at C2, C3, and C6 of mannose promote the deprotonation of the anomeric hydroxyl group by Cs2CO3 and chelating interactions between Cs and these oxygen atoms favour the formation of equatorial anomeric alkoxides, leading to the highly β-selective anomeric O-alkylation.
32431565	1	31	theme	D-mannose-derived	198:214	arg1	D-mannoses					301:310	conformationally restricted bicyclic D-mannoses	264:310	conformationally restricted bicyclic D-mannoses	264:310	A number of structurally diverse D-mannose-derived lactols, including various deoxy-D-mannoses and conformationally restricted bicyclic D-mannoses, have been synthesized and investigated in mechanistic studies of β-mannosylation via Cs2CO3-mediated anomeric O-alkylation.
32431565	1	31	theme	D-mannose-derived	198:214	arg1	deoxy-D-mannoses					243:258	various deoxy-D-mannoses	235:258	various deoxy-D-mannoses	235:258	A number of structurally diverse D-mannose-derived lactols, including various deoxy-D-mannoses and conformationally restricted bicyclic D-mannoses, have been synthesized and investigated in mechanistic studies of β-mannosylation via Cs2CO3-mediated anomeric O-alkylation.
32431565	1	31	theme	D-mannose-derived	198:214	arg1	lactols					216:222	structurally diverse D-mannose-derived lactols	177:222	structurally diverse D-mannose-derived lactols	177:222	A number of structurally diverse D-mannose-derived lactols, including various deoxy-D-mannoses and conformationally restricted bicyclic D-mannoses, have been synthesized and investigated in mechanistic studies of β-mannosylation via Cs2CO3-mediated anomeric O-alkylation.
32431565	5	32	theme	reactive	931:938	arg1	alkoxides					910:918	monomeric cesium alkoxides	893:918	monomeric cesium alkoxides	893:918	Reaction progress kinetic analysis suggested that monomeric cesium alkoxides be the key reactive species for alkylation with electrophiles.
32431565	5	32	theme	reactive	931:938	arg1	species					940:946	the key reactive species	923:946	the key reactive species for alkylation with electrophiles	923:980	Reaction progress kinetic analysis suggested that monomeric cesium alkoxides be the key reactive species for alkylation with electrophiles.
32431565	0	33	theme	Complex	127:133	arg1	Glycans					156:162	Complex Fucosylated N-Linked Glycans	127:162	Complex Fucosylated N-Linked Glycans	127:162	β-Mannosylation via O-Alkylation of Anomeric Cesium Alkoxides: Mechanistic Studies and Synthesis of the Hexasaccharide Core of Complex Fucosylated N-Linked Glycans.
32431565	8	34	theme	N-linked	1542:1549	arg1	glycans					1551:1557	complex fucosylated N-linked glycans	1522:1557	complex fucosylated N-linked glycans	1522:1557	The utilization of this β-mannosylation was demonstrated by an efficient synthesis of the hexasaccharide core of complex fucosylated N-linked glycans.
32431565	8	35	theme	β-mannosylation	1433:1447	arg1	utilization					1413:1423	The utilization	1409:1423	The utilization of this β-mannosylation	1409:1447	The utilization of this β-mannosylation was demonstrated by an efficient synthesis of the hexasaccharide core of complex fucosylated N-linked glycans.
32431565	1	36	theme	lactols	216:222	arg1	D-mannoses					301:310	conformationally restricted bicyclic D-mannoses	264:310	conformationally restricted bicyclic D-mannoses	264:310	A number of structurally diverse D-mannose-derived lactols, including various deoxy-D-mannoses and conformationally restricted bicyclic D-mannoses, have been synthesized and investigated in mechanistic studies of β-mannosylation via Cs2CO3-mediated anomeric O-alkylation.
32431565	1	36	theme	lactols	216:222	arg1	deoxy-D-mannoses					243:258	various deoxy-D-mannoses	235:258	various deoxy-D-mannoses	235:258	A number of structurally diverse D-mannose-derived lactols, including various deoxy-D-mannoses and conformationally restricted bicyclic D-mannoses, have been synthesized and investigated in mechanistic studies of β-mannosylation via Cs2CO3-mediated anomeric O-alkylation.
32431565	1	36	theme	lactols	216:222	arg1	number					167:172	A number	165:172	A number of structurally diverse D-mannose-derived lactols, including various deoxy-D-mannoses and conformationally restricted bicyclic D-mannoses,	165:311	A number of structurally diverse D-mannose-derived lactols, including various deoxy-D-mannoses and conformationally restricted bicyclic D-mannoses, have been synthesized and investigated in mechanistic studies of β-mannosylation via Cs2CO3-mediated anomeric O-alkylation.
32431565	8	37	theme	hexasaccharide	1499:1512	arg1	core					1514:1517	the hexasaccharide core	1495:1517	the hexasaccharide core of complex fucosylated N-linked glycans	1495:1557	The utilization of this β-mannosylation was demonstrated by an efficient synthesis of the hexasaccharide core of complex fucosylated N-linked glycans.
32431565	8	38	theme	core	1514:1517	arg1	synthesis					1482:1490	an efficient synthesis	1469:1490	an efficient synthesis of the hexasaccharide core of complex fucosylated N-linked glycans	1469:1557	The utilization of this β-mannosylation was demonstrated by an efficient synthesis of the hexasaccharide core of complex fucosylated N-linked glycans.
32431565	2	39	theme	restricted	490:499	arg1	D-mannoses					510:519	conformationally restricted bicyclic D-mannoses	473:519	conformationally restricted bicyclic D-mannoses	473:519	It was found that deoxy mannoses or conformationally restricted bicyclic D-mannoses are not as reactive as their corresponding parent mannose.
32431565	0	40	theme	Cesium	45:50	arg1	Alkoxides					52:60	Anomeric Cesium Alkoxides	36:60	Anomeric Cesium Alkoxides	36:60	β-Mannosylation via O-Alkylation of Anomeric Cesium Alkoxides: Mechanistic Studies and Synthesis of the Hexasaccharide Core of Complex Fucosylated N-Linked Glycans.
32431565	0	41	theme	N-Linked	147:154	arg1	Glycans					156:162	Complex Fucosylated N-Linked Glycans	127:162	Complex Fucosylated N-Linked Glycans	127:162	β-Mannosylation via O-Alkylation of Anomeric Cesium Alkoxides: Mechanistic Studies and Synthesis of the Hexasaccharide Core of Complex Fucosylated N-Linked Glycans.
32431565	8	42	theme	fucosylated	1530:1540	arg1	glycans					1551:1557	complex fucosylated N-linked glycans	1522:1557	complex fucosylated N-linked glycans	1522:1557	The utilization of this β-mannosylation was demonstrated by an efficient synthesis of the hexasaccharide core of complex fucosylated N-linked glycans.
32431565	4	43	theme	cesium	751:756	arg1	alkoxides					758:766	D-mannose-derived anomeric cesium alkoxides	724:766	D-mannose-derived anomeric cesium alkoxides	724:766	NMR studies of D-mannose-derived anomeric cesium alkoxides indicated the predominance of the equatorial β-anomer after deprotonation.
32431565	0	44	theme	Anomeric	36:43	arg1	Alkoxides					52:60	Anomeric Cesium Alkoxides	36:60	Anomeric Cesium Alkoxides	36:60	β-Mannosylation via O-Alkylation of Anomeric Cesium Alkoxides: Mechanistic Studies and Synthesis of the Hexasaccharide Core of Complex Fucosylated N-Linked Glycans.
32431565	6	45	theme	mannose	1049:1055	arg1	C3					1035:1036	C3	1035:1036	C3	1035:1036	DFT calculations supported that oxygen atoms at C2, C3, and C6 of mannose promote the deprotonation of the anomeric hydroxyl group by Cs2CO3 and chelating interactions between Cs and these oxygen atoms favour the formation of equatorial anomeric alkoxides, leading to the highly β-selective anomeric O-alkylation.
32431565	6	45	theme	mannose	1049:1055	arg1	C6					1043:1044	C6	1043:1044	C6	1043:1044	DFT calculations supported that oxygen atoms at C2, C3, and C6 of mannose promote the deprotonation of the anomeric hydroxyl group by Cs2CO3 and chelating interactions between Cs and these oxygen atoms favour the formation of equatorial anomeric alkoxides, leading to the highly β-selective anomeric O-alkylation.
32431565	6	45	theme	mannose	1049:1055	arg1	C2					1031:1032	C2	1031:1032	C2	1031:1032	DFT calculations supported that oxygen atoms at C2, C3, and C6 of mannose promote the deprotonation of the anomeric hydroxyl group by Cs2CO3 and chelating interactions between Cs and these oxygen atoms favour the formation of equatorial anomeric alkoxides, leading to the highly β-selective anomeric O-alkylation.
32431565	0	46	theme	Fucosylated	135:145	arg1	Glycans					156:162	Complex Fucosylated N-Linked Glycans	127:162	Complex Fucosylated N-Linked Glycans	127:162	β-Mannosylation via O-Alkylation of Anomeric Cesium Alkoxides: Mechanistic Studies and Synthesis of the Hexasaccharide Core of Complex Fucosylated N-Linked Glycans.
32431565	6	47	theme	DFT	983:985	arg1	calculations					987:998	DFT calculations	983:998	DFT calculations	983:998	DFT calculations supported that oxygen atoms at C2, C3, and C6 of mannose promote the deprotonation of the anomeric hydroxyl group by Cs2CO3 and chelating interactions between Cs and these oxygen atoms favour the formation of equatorial anomeric alkoxides, leading to the highly β-selective anomeric O-alkylation.
32431565	3	48	theme	that	677:680	arg1	protection					663:672	protection	663:672	protection of that	663:680	This type of β-mannosylation proceeds efficiently when the C2-OH is left free, and protection of that leads to inferior results.
32431565	1	49	theme	various	235:241	arg1	deoxy-D-mannoses					243:258	various deoxy-D-mannoses	235:258	various deoxy-D-mannoses	235:258	A number of structurally diverse D-mannose-derived lactols, including various deoxy-D-mannoses and conformationally restricted bicyclic D-mannoses, have been synthesized and investigated in mechanistic studies of β-mannosylation via Cs2CO3-mediated anomeric O-alkylation.
32431565	1	50	theme	mechanistic	355:365	arg1	studies					367:373	mechanistic studies	355:373	mechanistic studies of β-mannosylation	355:392	A number of structurally diverse D-mannose-derived lactols, including various deoxy-D-mannoses and conformationally restricted bicyclic D-mannoses, have been synthesized and investigated in mechanistic studies of β-mannosylation via Cs2CO3-mediated anomeric O-alkylation.
32431565	6	51	from	C6	1043:1044	arg1	atoms					1022:1026	oxygen atoms	1015:1026	oxygen atoms at C2, C3, and C6 of mannose	1015:1055	DFT calculations supported that oxygen atoms at C2, C3, and C6 of mannose promote the deprotonation of the anomeric hydroxyl group by Cs2CO3 and chelating interactions between Cs and these oxygen atoms favour the formation of equatorial anomeric alkoxides, leading to the highly β-selective anomeric O-alkylation.
32431565	6	52	from	C2	1031:1032	arg1	atoms					1022:1026	oxygen atoms	1015:1026	oxygen atoms at C2, C3, and C6 of mannose	1015:1055	DFT calculations supported that oxygen atoms at C2, C3, and C6 of mannose promote the deprotonation of the anomeric hydroxyl group by Cs2CO3 and chelating interactions between Cs and these oxygen atoms favour the formation of equatorial anomeric alkoxides, leading to the highly β-selective anomeric O-alkylation.
32431565	8	53	theme	glycans	1551:1557	arg1	core					1514:1517	the hexasaccharide core	1495:1517	the hexasaccharide core of complex fucosylated N-linked glycans	1495:1557	The utilization of this β-mannosylation was demonstrated by an efficient synthesis of the hexasaccharide core of complex fucosylated N-linked glycans.
32431565	6	54	theme	equatorial	1209:1218	arg1	alkoxides					1229:1237	equatorial anomeric alkoxides	1209:1237	equatorial anomeric alkoxides	1209:1237	DFT calculations supported that oxygen atoms at C2, C3, and C6 of mannose promote the deprotonation of the anomeric hydroxyl group by Cs2CO3 and chelating interactions between Cs and these oxygen atoms favour the formation of equatorial anomeric alkoxides, leading to the highly β-selective anomeric O-alkylation.
32431565	4	55	theme	equatorial	802:811	arg1	β-anomer					813:820	the equatorial β-anomer	798:820	the equatorial β-anomer	798:820	NMR studies of D-mannose-derived anomeric cesium alkoxides indicated the predominance of the equatorial β-anomer after deprotonation.
32431565	0	56	theme	Alkoxides	52:60	arg1	β-Mannosylation					0:14	β-Mannosylation	0:14	β-Mannosylation via O-Alkylation of Anomeric Cesium Alkoxides: Mechanistic Studies and Synthesis of the Hexasaccharide Core of Complex Fucosylated N-Linked Glycans.	0:163	β-Mannosylation via O-Alkylation of Anomeric Cesium Alkoxides: Mechanistic Studies and Synthesis of the Hexasaccharide Core of Complex Fucosylated N-Linked Glycans.
32431565	0	57	theme	Glycans	156:162	arg1	Core					119:122	the Hexasaccharide Core	100:122	the Hexasaccharide Core of Complex Fucosylated N-Linked Glycans	100:162	β-Mannosylation via O-Alkylation of Anomeric Cesium Alkoxides: Mechanistic Studies and Synthesis of the Hexasaccharide Core of Complex Fucosylated N-Linked Glycans.
32431565	4	58	link	D-mannose-derived	724:740	arg1	alkoxides					758:766	D-mannose-derived anomeric cesium alkoxides	724:766	D-mannose-derived anomeric cesium alkoxides	724:766	NMR studies of D-mannose-derived anomeric cesium alkoxides indicated the predominance of the equatorial β-anomer after deprotonation.
32431565	3	59	theme	inferior	691:698	arg1	results					700:706	inferior results	691:706	inferior results	691:706	This type of β-mannosylation proceeds efficiently when the C2-OH is left free, and protection of that leads to inferior results.
32431565	8	60	theme	complex	1522:1528	arg1	glycans					1551:1557	complex fucosylated N-linked glycans	1522:1557	complex fucosylated N-linked glycans	1522:1557	The utilization of this β-mannosylation was demonstrated by an efficient synthesis of the hexasaccharide core of complex fucosylated N-linked glycans.
32431565	6	61	theme	alkoxides	1229:1237	arg1	formation					1196:1204	the formation	1192:1204	the formation of equatorial anomeric alkoxides	1192:1237	DFT calculations supported that oxygen atoms at C2, C3, and C6 of mannose promote the deprotonation of the anomeric hydroxyl group by Cs2CO3 and chelating interactions between Cs and these oxygen atoms favour the formation of equatorial anomeric alkoxides, leading to the highly β-selective anomeric O-alkylation.
32431565	5	62	theme	Reaction	843:850	arg1	analysis					869:876	Reaction progress kinetic analysis	843:876	Reaction progress kinetic analysis	843:876	Reaction progress kinetic analysis suggested that monomeric cesium alkoxides be the key reactive species for alkylation with electrophiles.
32431565	5	63	theme	monomeric	893:901	arg1	alkoxides					910:918	monomeric cesium alkoxides	893:918	monomeric cesium alkoxides	893:918	Reaction progress kinetic analysis suggested that monomeric cesium alkoxides be the key reactive species for alkylation with electrophiles.
32431565	5	63	theme	monomeric	893:901	arg1	species					940:946	the key reactive species	923:946	the key reactive species for alkylation with electrophiles	923:980	Reaction progress kinetic analysis suggested that monomeric cesium alkoxides be the key reactive species for alkylation with electrophiles.
32431565	4	64	theme	β-anomer	813:820	arg1	predominance					782:793	the predominance	778:793	the predominance of the equatorial β-anomer	778:820	NMR studies of D-mannose-derived anomeric cesium alkoxides indicated the predominance of the equatorial β-anomer after deprotonation.
32431565	8	65	link	N-linked	1542:1549	arg1	glycans					1551:1557	complex fucosylated N-linked glycans	1522:1557	complex fucosylated N-linked glycans	1522:1557	The utilization of this β-mannosylation was demonstrated by an efficient synthesis of the hexasaccharide core of complex fucosylated N-linked glycans.
32431565	5	66	theme	kinetic	861:867	arg1	analysis					869:876	Reaction progress kinetic analysis	843:876	Reaction progress kinetic analysis	843:876	Reaction progress kinetic analysis suggested that monomeric cesium alkoxides be the key reactive species for alkylation with electrophiles.
32431565	2	67	theme	bicyclic	501:508	arg1	D-mannoses					510:519	conformationally restricted bicyclic D-mannoses	473:519	conformationally restricted bicyclic D-mannoses	473:519	It was found that deoxy mannoses or conformationally restricted bicyclic D-mannoses are not as reactive as their corresponding parent mannose.
32431565	3	68	theme	β-mannosylation	593:607	arg1	type					585:588	This type	580:588	This type of β-mannosylation	580:607	This type of β-mannosylation proceeds efficiently when the C2-OH is left free, and protection of that leads to inferior results.
32431565	4	69	theme	D-mannose-derived	724:740	arg1	alkoxides					758:766	D-mannose-derived anomeric cesium alkoxides	724:766	D-mannose-derived anomeric cesium alkoxides	724:766	NMR studies of D-mannose-derived anomeric cesium alkoxides indicated the predominance of the equatorial β-anomer after deprotonation.
32431565	0	70	theme	Mechanistic	63:73	arg1	Studies					75:81	Mechanistic Studies	63:81	Mechanistic Studies	63:81	β-Mannosylation via O-Alkylation of Anomeric Cesium Alkoxides: Mechanistic Studies and Synthesis of the Hexasaccharide Core of Complex Fucosylated N-Linked Glycans.
32431565	1	71	theme	β-mannosylation	378:392	arg1	studies					367:373	mechanistic studies	355:373	mechanistic studies of β-mannosylation	355:392	A number of structurally diverse D-mannose-derived lactols, including various deoxy-D-mannoses and conformationally restricted bicyclic D-mannoses, have been synthesized and investigated in mechanistic studies of β-mannosylation via Cs2CO3-mediated anomeric O-alkylation.
32431565	0	72	dep	β-Mannosylation	0:14	arg1	Synthesis					87:95	Synthesis	87:95	Synthesis	87:95	β-Mannosylation via O-Alkylation of Anomeric Cesium Alkoxides: Mechanistic Studies and Synthesis of the Hexasaccharide Core of Complex Fucosylated N-Linked Glycans.
32431565	0	72	dep	β-Mannosylation	0:14	arg1	Studies					75:81	Mechanistic Studies	63:81	Mechanistic Studies	63:81	β-Mannosylation via O-Alkylation of Anomeric Cesium Alkoxides: Mechanistic Studies and Synthesis of the Hexasaccharide Core of Complex Fucosylated N-Linked Glycans.
32431565	5	73	with	alkylation	952:961	arg1	electrophiles					968:980	electrophiles	968:980	electrophiles	968:980	Reaction progress kinetic analysis suggested that monomeric cesium alkoxides be the key reactive species for alkylation with electrophiles.
32431565	1	74	theme	restricted	281:290	arg1	D-mannoses					301:310	conformationally restricted bicyclic D-mannoses	264:310	conformationally restricted bicyclic D-mannoses	264:310	A number of structurally diverse D-mannose-derived lactols, including various deoxy-D-mannoses and conformationally restricted bicyclic D-mannoses, have been synthesized and investigated in mechanistic studies of β-mannosylation via Cs2CO3-mediated anomeric O-alkylation.
34248864	7	0	theme	ABC	1464:1466	arg1	transporters					1468:1479	ABC transporters	1464:1479	LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters	1353:1479	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	1	1	theme	gastrointestinal	128:143	arg1	tract					145:149	The human gastrointestinal tract	118:149	The human gastrointestinal tract	118:149	The human gastrointestinal tract is colonized by a diverse microbial community, which plays a crucial role in human health.
34248864	8	2	theme	germ-free	1488:1496	arg1	mice					1498:1501	germ-free mice	1488:1501	germ-free mice colonized with B. thetaiotaomicron and/or A. muciniphila	1488:1558	Using germ-free mice colonized with B. thetaiotaomicron and/or A. muciniphila, we observed a more general glycan degrading profile in B. thetaiotaomicron while the expression profile of A. muciniphila was not significantly affected when colonizing together, indicating that two different nutritional niches were established in mice gut.
34248864	3	3	from	compound	456:463	arg1	tract					489:493	the gastrointestinal tract	468:493	the gastrointestinal tract	468:493	These host-derived glycans are utilized by bacteria that have adapted to this specific compound in the gastrointestinal tract.
34248864	2	4	theme	glycan	296:301	arg1	structures					303:312	glycan structures	296:312	glycan structures	296:312	In the gut, a protective mucus layer that consists of glycan structures separates the bacteria from the host epithelial cells.
34248864	5	5	theme	mucin	896:900	arg1	activity					914:921	mucin degradation activity	896:921	mucin degradation activity	896:921	The in vitro study, in which mucin was the only nutrient source, indicated that B. thetaiotaomicron significantly upregulated genes coding for Glycoside Hydrolases (GHs) and mucin degradation activity when cultured in the presence of A. muciniphila.
34248864	6	6	theme	membrane	1069:1076	arg1	domain					1110:1115	membrane attack complex/perforin (MACPF) domain	1069:1115	membrane attack complex/perforin (MACPF) domain in co-culture	1069:1129	Furthermore, B. thetaiotaomicron significantly upregulated the expression of a gene encoding for membrane attack complex/perforin (MACPF) domain in co-culture.
34248864	7	7	theme	thetaiotaomicron	1301:1316	arg1	presence					1286:1293	the presence	1282:1293	the presence of B. thetaiotaomicron	1282:1316	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	8	8	theme	mice	1809:1812	arg1	gut					1814:1816	mice gut	1809:1816	mice gut	1809:1816	Using germ-free mice colonized with B. thetaiotaomicron and/or A. muciniphila, we observed a more general glycan degrading profile in B. thetaiotaomicron while the expression profile of A. muciniphila was not significantly affected when colonizing together, indicating that two different nutritional niches were established in mice gut.
34248864	7	9	theme	Lipid	1418:1422	arg1	genes					1393:1397	LPS core biosynthesis activity encoding genes	1353:1397	LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters	1353:1479	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	7	9	theme	Lipid	1418:1422	arg1	A					1424:1424	Lipid A	1418:1424	Lipid A	1418:1424	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	4	10	theme	generalist	617:626	arg1	members					577:583	two distinct gut microbiota members	549:583	two distinct gut microbiota members known to use mucus glycans	549:610	Our study investigated the close interaction between two distinct gut microbiota members known to use mucus glycans, the generalist Bacteroides thetaiotaomicron and the specialist Akkermansia muciniphila in vitro and in vivo.
34248864	4	10	theme	generalist	617:626	arg1	thetaiotaomicron					640:655	the generalist Bacteroides thetaiotaomicron	613:655	the generalist Bacteroides thetaiotaomicron	613:655	Our study investigated the close interaction between two distinct gut microbiota members known to use mucus glycans, the generalist Bacteroides thetaiotaomicron and the specialist Akkermansia muciniphila in vitro and in vivo.
34248864	1	11	theme	human	228:232	arg1	health					234:239	human health	228:239	human health	228:239	The human gastrointestinal tract is colonized by a diverse microbial community, which plays a crucial role in human health.
34248864	0	12	theme	Glycan	78:83	arg1	Generalist					85:94	a Glycan Generalist	76:94	a Glycan Generalist	76:94	A Continuous Battle for Host-Derived Glycans Between a Mucus Specialist and a Glycan Generalist in vitro and in vivo.
34248864	9	13	theme	mucin	1961:1965	arg1	specialist					1977:1986	a mucin degrading specialist	1959:1986	a mucin degrading specialist	1959:1986	Thus, our results indicate that a mucin degrading generalist adapts to its changing environment, depending on available carbohydrates while a mucin degrading specialist adapts by coping with competing microorganism through upregulation of defense related genes.
34248864	4	14	theme	Akkermansia	676:686	arg1	members					577:583	two distinct gut microbiota members	549:583	two distinct gut microbiota members known to use mucus glycans	549:610	Our study investigated the close interaction between two distinct gut microbiota members known to use mucus glycans, the generalist Bacteroides thetaiotaomicron and the specialist Akkermansia muciniphila in vitro and in vivo.
34248864	4	14	theme	Akkermansia	676:686	arg1	muciniphila					688:698	the specialist Akkermansia muciniphila	661:698	the specialist Akkermansia muciniphila	661:698	Our study investigated the close interaction between two distinct gut microbiota members known to use mucus glycans, the generalist Bacteroides thetaiotaomicron and the specialist Akkermansia muciniphila in vitro and in vivo.
34248864	9	15	theme	changing	1894:1901	arg1	environment					1903:1913	its changing environment	1890:1913	its changing environment	1890:1913	Thus, our results indicate that a mucin degrading generalist adapts to its changing environment, depending on available carbohydrates while a mucin degrading specialist adapts by coping with competing microorganism through upregulation of defense related genes.
34248864	4	16	theme	distinct	553:560	arg1	members					577:583	two distinct gut microbiota members	549:583	two distinct gut microbiota members known to use mucus glycans	549:610	Our study investigated the close interaction between two distinct gut microbiota members known to use mucus glycans, the generalist Bacteroides thetaiotaomicron and the specialist Akkermansia muciniphila in vitro and in vivo.
34248864	4	16	theme	distinct	553:560	arg1	muciniphila					688:698	the specialist Akkermansia muciniphila	661:698	the specialist Akkermansia muciniphila	661:698	Our study investigated the close interaction between two distinct gut microbiota members known to use mucus glycans, the generalist Bacteroides thetaiotaomicron and the specialist Akkermansia muciniphila in vitro and in vivo.
34248864	4	16	theme	distinct	553:560	arg1	thetaiotaomicron					640:655	the generalist Bacteroides thetaiotaomicron	613:655	the generalist Bacteroides thetaiotaomicron	613:655	Our study investigated the close interaction between two distinct gut microbiota members known to use mucus glycans, the generalist Bacteroides thetaiotaomicron and the specialist Akkermansia muciniphila in vitro and in vivo.
34248864	5	17	from	source	779:784	arg1	study					735:739	The in vitro study	722:739	The in vitro study	722:739	The in vitro study, in which mucin was the only nutrient source, indicated that B. thetaiotaomicron significantly upregulated genes coding for Glycoside Hydrolases (GHs) and mucin degradation activity when cultured in the presence of A. muciniphila.
34248864	3	18	link	host-derived	375:386	arg1	glycans					388:394	These host-derived glycans	369:394	These host-derived glycans	369:394	These host-derived glycans are utilized by bacteria that have adapted to this specific compound in the gastrointestinal tract.
34248864	3	19	used	utilized	400:407	arg2	glycans					388:394	These host-derived glycans	369:394	These host-derived glycans	369:394	These host-derived glycans are utilized by bacteria that have adapted to this specific compound in the gastrointestinal tract.
34248864	2	20	theme	host	346:349	arg1	cells					362:366	the host epithelial cells	342:366	the host epithelial cells	342:366	In the gut, a protective mucus layer that consists of glycan structures separates the bacteria from the host epithelial cells.
34248864	8	21	theme	B.	1616:1617	arg1	thetaiotaomicron					1619:1634	B. thetaiotaomicron	1616:1634	B. thetaiotaomicron	1616:1634	Using germ-free mice colonized with B. thetaiotaomicron and/or A. muciniphila, we observed a more general glycan degrading profile in B. thetaiotaomicron while the expression profile of A. muciniphila was not significantly affected when colonizing together, indicating that two different nutritional niches were established in mice gut.
34248864	5	22	theme	Glycoside	865:873	arg1	GHs					887:889	GHs	887:889	GHs	887:889	The in vitro study, in which mucin was the only nutrient source, indicated that B. thetaiotaomicron significantly upregulated genes coding for Glycoside Hydrolases (GHs) and mucin degradation activity when cultured in the presence of A. muciniphila.
34248864	5	22	theme	Glycoside	865:873	arg1	Hydrolases					875:884	Glycoside Hydrolases	865:884	Glycoside Hydrolases (GHs)	865:890	The in vitro study, in which mucin was the only nutrient source, indicated that B. thetaiotaomicron significantly upregulated genes coding for Glycoside Hydrolases (GHs) and mucin degradation activity when cultured in the presence of A. muciniphila.
34248864	6	23	theme	gene	1051:1054	arg1	expression					1035:1044	the expression	1031:1044	the expression of a gene encoding for membrane attack complex/perforin (MACPF) domain in co-culture	1031:1129	Furthermore, B. thetaiotaomicron significantly upregulated the expression of a gene encoding for membrane attack complex/perforin (MACPF) domain in co-culture.
34248864	0	24	theme	Continuous	2:11	arg1	Battle					13:18	A Continuous Battle	0:18	A Continuous Battle for Host-Derived Glycans Between a Mucus Specialist and a Glycan Generalist in vitro and in vivo.	0:116	A Continuous Battle for Host-Derived Glycans Between a Mucus Specialist and a Glycan Generalist in vitro and in vivo.
34248864	7	25	theme	Glycosyl	1430:1437	arg1	transferases					1439:1450	Glycosyl transferases	1430:1450	Glycosyl transferases	1430:1450	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	9	26	theme	defense	2058:2064	arg1	genes					2074:2078	defense related genes	2058:2078	defense related genes	2058:2078	Thus, our results indicate that a mucin degrading generalist adapts to its changing environment, depending on available carbohydrates while a mucin degrading specialist adapts by coping with competing microorganism through upregulation of defense related genes.
34248864	2	27	theme	mucus	267:271	arg1	layer					273:277	a protective mucus layer	254:277	a protective mucus layer that consists of glycan structures	254:312	In the gut, a protective mucus layer that consists of glycan structures separates the bacteria from the host epithelial cells.
34248864	3	28	theme	host-derived	375:386	arg1	glycans					388:394	These host-derived glycans	369:394	These host-derived glycans	369:394	These host-derived glycans are utilized by bacteria that have adapted to this specific compound in the gastrointestinal tract.
34248864	5	29	theme	only	765:768	arg1	mucin					751:755	mucin	751:755	mucin	751:755	The in vitro study, in which mucin was the only nutrient source, indicated that B. thetaiotaomicron significantly upregulated genes coding for Glycoside Hydrolases (GHs) and mucin degradation activity when cultured in the presence of A. muciniphila.
34248864	5	29	theme	only	765:768	arg1	source					779:784	the only nutrient source	761:784	the only nutrient source	761:784	The in vitro study, in which mucin was the only nutrient source, indicated that B. thetaiotaomicron significantly upregulated genes coding for Glycoside Hydrolases (GHs) and mucin degradation activity when cultured in the presence of A. muciniphila.
34248864	9	30	theme	related	2066:2072	arg1	genes					2074:2078	defense related genes	2058:2078	defense related genes	2058:2078	Thus, our results indicate that a mucin degrading generalist adapts to its changing environment, depending on available carbohydrates while a mucin degrading specialist adapts by coping with competing microorganism through upregulation of defense related genes.
34248864	2	31	theme	protective	256:265	arg1	layer					273:277	a protective mucus layer	254:277	a protective mucus layer that consists of glycan structures	254:312	In the gut, a protective mucus layer that consists of glycan structures separates the bacteria from the host epithelial cells.
34248864	4	32	theme	Bacteroides	628:638	arg1	members					577:583	two distinct gut microbiota members	549:583	two distinct gut microbiota members known to use mucus glycans	549:610	Our study investigated the close interaction between two distinct gut microbiota members known to use mucus glycans, the generalist Bacteroides thetaiotaomicron and the specialist Akkermansia muciniphila in vitro and in vivo.
34248864	4	32	theme	Bacteroides	628:638	arg1	thetaiotaomicron					640:655	the generalist Bacteroides thetaiotaomicron	613:655	the generalist Bacteroides thetaiotaomicron	613:655	Our study investigated the close interaction between two distinct gut microbiota members known to use mucus glycans, the generalist Bacteroides thetaiotaomicron and the specialist Akkermansia muciniphila in vitro and in vivo.
34248864	5	33	theme	nutrient	770:777	arg1	mucin					751:755	mucin	751:755	mucin	751:755	The in vitro study, in which mucin was the only nutrient source, indicated that B. thetaiotaomicron significantly upregulated genes coding for Glycoside Hydrolases (GHs) and mucin degradation activity when cultured in the presence of A. muciniphila.
34248864	5	33	theme	nutrient	770:777	arg1	source					779:784	the only nutrient source	761:784	the only nutrient source	761:784	The in vitro study, in which mucin was the only nutrient source, indicated that B. thetaiotaomicron significantly upregulated genes coding for Glycoside Hydrolases (GHs) and mucin degradation activity when cultured in the presence of A. muciniphila.
34248864	7	34	theme	O-antigen	1400:1408	arg1	genes					1393:1397	LPS core biosynthesis activity encoding genes	1353:1397	LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters	1353:1479	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	7	34	theme	O-antigen	1400:1408	arg1	ligase					1410:1415	O-antigen ligase	1400:1415	O-antigen ligase	1400:1415	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	9	35	theme	genes	2074:2078	arg1	upregulation					2042:2053	upregulation	2042:2053	upregulation of defense related genes	2042:2078	Thus, our results indicate that a mucin degrading generalist adapts to its changing environment, depending on available carbohydrates while a mucin degrading specialist adapts by coping with competing microorganism through upregulation of defense related genes.
34248864	7	36	theme	encoding	1384:1391	arg1	genes					1393:1397	LPS core biosynthesis activity encoding genes	1353:1397	LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters	1353:1479	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	7	36	theme	encoding	1384:1391	arg1	ligase					1410:1415	O-antigen ligase	1400:1415	O-antigen ligase	1400:1415	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	7	36	theme	encoding	1384:1391	arg1	A					1424:1424	Lipid A	1418:1424	Lipid A	1418:1424	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	9	37	theme	available	1929:1937	arg1	carbohydrates					1939:1951	available carbohydrates	1929:1951	available carbohydrates	1929:1951	Thus, our results indicate that a mucin degrading generalist adapts to its changing environment, depending on available carbohydrates while a mucin degrading specialist adapts by coping with competing microorganism through upregulation of defense related genes.
34248864	1	38	theme	diverse	169:175	arg1	community					187:195	a diverse microbial community	167:195	a diverse microbial community	167:195	The human gastrointestinal tract is colonized by a diverse microbial community, which plays a crucial role in human health.
34248864	5	39	theme	degradation	902:912	arg1	activity					914:921	mucin degradation activity	896:921	mucin degradation activity	896:921	The in vitro study, in which mucin was the only nutrient source, indicated that B. thetaiotaomicron significantly upregulated genes coding for Glycoside Hydrolases (GHs) and mucin degradation activity when cultured in the presence of A. muciniphila.
34248864	7	40	dep	genes	1393:1397	arg1	transferases					1439:1450	Glycosyl transferases	1430:1450	Glycosyl transferases	1430:1450	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	7	40	dep	genes	1393:1397	arg1	genes					1393:1397	LPS core biosynthesis activity encoding genes	1353:1397	LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters	1353:1479	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	7	40	dep	genes	1393:1397	arg1	ligase					1410:1415	O-antigen ligase	1400:1415	O-antigen ligase	1400:1415	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	7	40	dep	genes	1393:1397	arg1	A					1424:1424	Lipid A	1418:1424	Lipid A	1418:1424	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	8	41	theme	nutritional	1770:1780	arg1	niches					1782:1787	two different nutritional niches	1756:1787	two different nutritional niches	1756:1787	Using germ-free mice colonized with B. thetaiotaomicron and/or A. muciniphila, we observed a more general glycan degrading profile in B. thetaiotaomicron while the expression profile of A. muciniphila was not significantly affected when colonizing together, indicating that two different nutritional niches were established in mice gut.
34248864	4	42	theme	specialist	665:674	arg1	members					577:583	two distinct gut microbiota members	549:583	two distinct gut microbiota members known to use mucus glycans	549:610	Our study investigated the close interaction between two distinct gut microbiota members known to use mucus glycans, the generalist Bacteroides thetaiotaomicron and the specialist Akkermansia muciniphila in vitro and in vivo.
34248864	4	42	theme	specialist	665:674	arg1	muciniphila					688:698	the specialist Akkermansia muciniphila	661:698	the specialist Akkermansia muciniphila	661:698	Our study investigated the close interaction between two distinct gut microbiota members known to use mucus glycans, the generalist Bacteroides thetaiotaomicron and the specialist Akkermansia muciniphila in vitro and in vivo.
34248864	1	43	theme	microbial	177:185	arg1	community					187:195	a diverse microbial community	167:195	a diverse microbial community	167:195	The human gastrointestinal tract is colonized by a diverse microbial community, which plays a crucial role in human health.
34248864	4	44	theme	close	523:527	arg1	interaction					529:539	the close interaction	519:539	the close interaction between two distinct gut microbiota members known to use mucus glycans, the generalist Bacteroides thetaiotaomicron and the specialist Akkermansia muciniphila	519:698	Our study investigated the close interaction between two distinct gut microbiota members known to use mucus glycans, the generalist Bacteroides thetaiotaomicron and the specialist Akkermansia muciniphila in vitro and in vivo.
34248864	5	45	dep	in	726:727	arg1	vitro					729:733	vitro	729:733	vitro	729:733	The in vitro study, in which mucin was the only nutrient source, indicated that B. thetaiotaomicron significantly upregulated genes coding for Glycoside Hydrolases (GHs) and mucin degradation activity when cultured in the presence of A. muciniphila.
34248864	7	46	theme	biosynthesis	1362:1373	arg1	genes					1393:1397	LPS core biosynthesis activity encoding genes	1353:1397	LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters	1353:1479	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	7	46	theme	biosynthesis	1362:1373	arg1	ligase					1410:1415	O-antigen ligase	1400:1415	O-antigen ligase	1400:1415	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	7	46	theme	biosynthesis	1362:1373	arg1	A					1424:1424	Lipid A	1418:1424	Lipid A	1418:1424	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	7	47	theme	B.	1298:1299	arg1	thetaiotaomicron					1301:1316	B. thetaiotaomicron	1298:1316	B. thetaiotaomicron	1298:1316	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	0	48	theme	Host-Derived	24:35	arg1	Glycans					37:43	Host-Derived Glycans	24:43	Host-Derived Glycans	24:43	A Continuous Battle for Host-Derived Glycans Between a Mucus Specialist and a Glycan Generalist in vitro and in vivo.
34248864	9	49	theme	degrading	1859:1867	arg1	generalist					1869:1878	a mucin degrading generalist	1851:1878	a mucin degrading generalist	1851:1878	Thus, our results indicate that a mucin degrading generalist adapts to its changing environment, depending on available carbohydrates while a mucin degrading specialist adapts by coping with competing microorganism through upregulation of defense related genes.
34248864	7	50	theme	activity	1375:1382	arg1	genes					1393:1397	LPS core biosynthesis activity encoding genes	1353:1397	LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters	1353:1479	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	7	50	theme	activity	1375:1382	arg1	ligase					1410:1415	O-antigen ligase	1400:1415	O-antigen ligase	1400:1415	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	7	50	theme	activity	1375:1382	arg1	A					1424:1424	Lipid A	1418:1424	Lipid A	1418:1424	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	2	51	theme	epithelial	351:360	arg1	cells					362:366	the host epithelial cells	342:366	the host epithelial cells	342:366	In the gut, a protective mucus layer that consists of glycan structures separates the bacteria from the host epithelial cells.
34248864	7	52	theme	LPS	1353:1355	arg1	genes					1393:1397	LPS core biosynthesis activity encoding genes	1353:1397	LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters	1353:1479	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	7	52	theme	LPS	1353:1355	arg1	ligase					1410:1415	O-antigen ligase	1400:1415	O-antigen ligase	1400:1415	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	7	52	theme	LPS	1353:1355	arg1	A					1424:1424	Lipid A	1418:1424	Lipid A	1418:1424	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	6	53	theme	attack	1078:1083	arg1	domain					1110:1115	membrane attack complex/perforin (MACPF) domain	1069:1115	membrane attack complex/perforin (MACPF) domain in co-culture	1069:1129	Furthermore, B. thetaiotaomicron significantly upregulated the expression of a gene encoding for membrane attack complex/perforin (MACPF) domain in co-culture.
34248864	7	54	dep	A.	1179:1180	arg1	muciniphila					1182:1192	A. muciniphila	1179:1192	A. muciniphila	1179:1192	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	4	55	theme	gut	562:564	arg1	members					577:583	two distinct gut microbiota members	549:583	two distinct gut microbiota members known to use mucus glycans	549:610	Our study investigated the close interaction between two distinct gut microbiota members known to use mucus glycans, the generalist Bacteroides thetaiotaomicron and the specialist Akkermansia muciniphila in vitro and in vivo.
34248864	4	55	theme	gut	562:564	arg1	muciniphila					688:698	the specialist Akkermansia muciniphila	661:698	the specialist Akkermansia muciniphila	661:698	Our study investigated the close interaction between two distinct gut microbiota members known to use mucus glycans, the generalist Bacteroides thetaiotaomicron and the specialist Akkermansia muciniphila in vitro and in vivo.
34248864	4	55	theme	gut	562:564	arg1	thetaiotaomicron					640:655	the generalist Bacteroides thetaiotaomicron	613:655	the generalist Bacteroides thetaiotaomicron	613:655	Our study investigated the close interaction between two distinct gut microbiota members known to use mucus glycans, the generalist Bacteroides thetaiotaomicron and the specialist Akkermansia muciniphila in vitro and in vivo.
34248864	7	56	theme	core	1357:1360	arg1	genes					1393:1397	LPS core biosynthesis activity encoding genes	1353:1397	LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters	1353:1479	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	7	56	theme	core	1357:1360	arg1	ligase					1410:1415	O-antigen ligase	1400:1415	O-antigen ligase	1400:1415	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	7	56	theme	core	1357:1360	arg1	A					1424:1424	Lipid A	1418:1424	Lipid A	1418:1424	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	6	57	from	domain	1110:1115	arg1	co-culture					1120:1129	co-culture	1120:1129	co-culture	1120:1129	Furthermore, B. thetaiotaomicron significantly upregulated the expression of a gene encoding for membrane attack complex/perforin (MACPF) domain in co-culture.
34248864	3	58	theme	specific	447:454	arg1	compound					456:463	this specific compound	442:463	this specific compound in the gastrointestinal tract	442:493	These host-derived glycans are utilized by bacteria that have adapted to this specific compound in the gastrointestinal tract.
34248864	7	59	theme	transcriptome	1136:1148	arg1	analysis					1150:1157	The transcriptome analysis	1132:1157	The transcriptome analysis	1132:1157	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	9	60	theme	degrading	1967:1975	arg1	specialist					1977:1986	a mucin degrading specialist	1959:1986	a mucin degrading specialist	1959:1986	Thus, our results indicate that a mucin degrading generalist adapts to its changing environment, depending on available carbohydrates while a mucin degrading specialist adapts by coping with competing microorganism through upregulation of defense related genes.
34248864	8	61	theme	muciniphila	1671:1681	arg1	profile					1657:1663	the expression profile	1642:1663	the expression profile of A. muciniphila	1642:1681	Using germ-free mice colonized with B. thetaiotaomicron and/or A. muciniphila, we observed a more general glycan degrading profile in B. thetaiotaomicron while the expression profile of A. muciniphila was not significantly affected when colonizing together, indicating that two different nutritional niches were established in mice gut.
34248864	6	62	theme	B.	985:986	arg1	thetaiotaomicron					988:1003	B. thetaiotaomicron	985:1003	B. thetaiotaomicron	985:1003	Furthermore, B. thetaiotaomicron significantly upregulated the expression of a gene encoding for membrane attack complex/perforin (MACPF) domain in co-culture.
34248864	6	63	theme	MACPF	1103:1107	arg1	domain					1110:1115	membrane attack complex/perforin (MACPF) domain	1069:1115	membrane attack complex/perforin (MACPF) domain in co-culture	1069:1129	Furthermore, B. thetaiotaomicron significantly upregulated the expression of a gene encoding for membrane attack complex/perforin (MACPF) domain in co-culture.
34248864	7	64	theme	genes	1393:1397	arg1	expression					1339:1348	the expression	1335:1348	the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters	1335:1479	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	8	65	theme	different	1760:1768	arg1	niches					1782:1787	two different nutritional niches	1756:1787	two different nutritional niches	1756:1787	Using germ-free mice colonized with B. thetaiotaomicron and/or A. muciniphila, we observed a more general glycan degrading profile in B. thetaiotaomicron while the expression profile of A. muciniphila was not significantly affected when colonizing together, indicating that two different nutritional niches were established in mice gut.
34248864	7	66	theme	transporters	1468:1479	arg1	expression					1339:1348	the expression	1335:1348	the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters	1335:1479	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	2	67	from	cells	362:366	arg1	bacteria					328:335	the bacteria	324:335	the bacteria from the host epithelial cells	324:366	In the gut, a protective mucus layer that consists of glycan structures separates the bacteria from the host epithelial cells.
34248864	1	68	theme	human	122:126	arg1	tract					145:149	The human gastrointestinal tract	118:149	The human gastrointestinal tract	118:149	The human gastrointestinal tract is colonized by a diverse microbial community, which plays a crucial role in human health.
34248864	9	69	theme	mucin	1853:1857	arg1	generalist					1869:1878	a mucin degrading generalist	1851:1878	a mucin degrading generalist	1851:1878	Thus, our results indicate that a mucin degrading generalist adapts to its changing environment, depending on available carbohydrates while a mucin degrading specialist adapts by coping with competing microorganism through upregulation of defense related genes.
34248864	4	70	theme	microbiota	566:575	arg1	members					577:583	two distinct gut microbiota members	549:583	two distinct gut microbiota members known to use mucus glycans	549:610	Our study investigated the close interaction between two distinct gut microbiota members known to use mucus glycans, the generalist Bacteroides thetaiotaomicron and the specialist Akkermansia muciniphila in vitro and in vivo.
34248864	4	70	theme	microbiota	566:575	arg1	muciniphila					688:698	the specialist Akkermansia muciniphila	661:698	the specialist Akkermansia muciniphila	661:698	Our study investigated the close interaction between two distinct gut microbiota members known to use mucus glycans, the generalist Bacteroides thetaiotaomicron and the specialist Akkermansia muciniphila in vitro and in vivo.
34248864	4	70	theme	microbiota	566:575	arg1	thetaiotaomicron					640:655	the generalist Bacteroides thetaiotaomicron	613:655	the generalist Bacteroides thetaiotaomicron	613:655	Our study investigated the close interaction between two distinct gut microbiota members known to use mucus glycans, the generalist Bacteroides thetaiotaomicron and the specialist Akkermansia muciniphila in vitro and in vivo.
34248864	5	71	theme	in	726:727	arg1	study					735:739	The in vitro study	722:739	The in vitro study	722:739	The in vitro study, in which mucin was the only nutrient source, indicated that B. thetaiotaomicron significantly upregulated genes coding for Glycoside Hydrolases (GHs) and mucin degradation activity when cultured in the presence of A. muciniphila.
34248864	5	72	theme	B.	802:803	arg1	thetaiotaomicron					805:820	B. thetaiotaomicron	802:820	B. thetaiotaomicron	802:820	The in vitro study, in which mucin was the only nutrient source, indicated that B. thetaiotaomicron significantly upregulated genes coding for Glycoside Hydrolases (GHs) and mucin degradation activity when cultured in the presence of A. muciniphila.
34248864	5	73	theme	muciniphila	959:969	arg1	presence					944:951	the presence	940:951	the presence of A. muciniphila	940:969	The in vitro study, in which mucin was the only nutrient source, indicated that B. thetaiotaomicron significantly upregulated genes coding for Glycoside Hydrolases (GHs) and mucin degradation activity when cultured in the presence of A. muciniphila.
34248864	4	74	theme	mucus	598:602	arg1	glycans					604:610	mucus glycans	598:610	mucus glycans	598:610	Our study investigated the close interaction between two distinct gut microbiota members known to use mucus glycans, the generalist Bacteroides thetaiotaomicron and the specialist Akkermansia muciniphila in vitro and in vivo.
34248864	6	75	theme	complex/perforin	1085:1100	arg1	domain					1110:1115	membrane attack complex/perforin (MACPF) domain	1069:1115	membrane attack complex/perforin (MACPF) domain in co-culture	1069:1129	Furthermore, B. thetaiotaomicron significantly upregulated the expression of a gene encoding for membrane attack complex/perforin (MACPF) domain in co-culture.
34248864	8	76	theme	expression	1646:1655	arg1	profile					1657:1663	the expression profile	1642:1663	the expression profile of A. muciniphila	1642:1681	Using germ-free mice colonized with B. thetaiotaomicron and/or A. muciniphila, we observed a more general glycan degrading profile in B. thetaiotaomicron while the expression profile of A. muciniphila was not significantly affected when colonizing together, indicating that two different nutritional niches were established in mice gut.
34248864	8	77	theme	degrading	1595:1603	arg1	profile					1605:1611	a more general glycan degrading profile	1573:1611	a more general glycan degrading profile	1573:1611	Using germ-free mice colonized with B. thetaiotaomicron and/or A. muciniphila, we observed a more general glycan degrading profile in B. thetaiotaomicron while the expression profile of A. muciniphila was not significantly affected when colonizing together, indicating that two different nutritional niches were established in mice gut.
34248864	0	78	theme	Mucus	55:59	arg1	Specialist					61:70	a Mucus Specialist	53:70	a Mucus Specialist	53:70	A Continuous Battle for Host-Derived Glycans Between a Mucus Specialist and a Glycan Generalist in vitro and in vivo.
34248864	8	79	theme	general	1580:1586	arg1	profile					1605:1611	a more general glycan degrading profile	1573:1611	a more general glycan degrading profile	1573:1611	Using germ-free mice colonized with B. thetaiotaomicron and/or A. muciniphila, we observed a more general glycan degrading profile in B. thetaiotaomicron while the expression profile of A. muciniphila was not significantly affected when colonizing together, indicating that two different nutritional niches were established in mice gut.
34248864	8	80	theme	glycan	1588:1593	arg1	profile					1605:1611	a more general glycan degrading profile	1573:1611	a more general glycan degrading profile	1573:1611	Using germ-free mice colonized with B. thetaiotaomicron and/or A. muciniphila, we observed a more general glycan degrading profile in B. thetaiotaomicron while the expression profile of A. muciniphila was not significantly affected when colonizing together, indicating that two different nutritional niches were established in mice gut.
34248864	3	81	theme	gastrointestinal	472:487	arg1	tract					489:493	the gastrointestinal tract	468:493	the gastrointestinal tract	468:493	These host-derived glycans are utilized by bacteria that have adapted to this specific compound in the gastrointestinal tract.
34248864	7	82	theme	environmental	1219:1231	arg1	changes					1233:1239	the environmental changes	1215:1239	the environmental changes	1215:1239	The transcriptome analysis also indicated that A. muciniphila was less affected by the environmental changes and was able to sustain its abundance in the presence of B. thetaiotaomicron while increasing the expression of LPS core biosynthesis activity encoding genes (O-antigen ligase, Lipid A and Glycosyl transferases) as well as ABC transporters.
34248864	1	83	theme	crucial	212:218	arg1	role					220:223	a crucial role	210:223	a crucial role	210:223	The human gastrointestinal tract is colonized by a diverse microbial community, which plays a crucial role in human health.
33674390	8	0	theme	variants	1350:1357	arg1	secretion					1318:1326	secretion	1318:1326	secretion	1318:1326	The N-glycan promotes folding and secretion of destabilized C-Pro variants by providing access to the ER's lectin-based chaperone machinery.
33674390	8	0	theme	variants	1350:1357	arg1	folding					1306:1312	folding	1306:1312	folding	1306:1312	The N-glycan promotes folding and secretion of destabilized C-Pro variants by providing access to the ER's lectin-based chaperone machinery.
33674390	11	1	theme	enigmatic	1818:1826	arg1	N-glycan					1828:1835	procollagen's previously enigmatic N-glycan	1793:1835	procollagen's previously enigmatic N-glycan	1793:1835	Collectively, these results establish an essential, context-dependent function for procollagen's previously enigmatic N-glycan, wherein the carbohydrate moiety buffers procollagen folding against proteostatic challenge.
33674390	1	2	theme	propeptide	157:166	arg1	domain					176:181	the protein's C-terminal propeptide (C-Pro) domain	132:181	the protein's C-terminal propeptide (C-Pro) domain	132:181	Intracellular procollagen folding begins at the protein's C-terminal propeptide (C-Pro) domain, which initiates triple-helix assembly and defines the composition and chain register of fibrillar collagen trimers.
33674390	6	3	theme	context-dependent	999:1015	arg1	function					1017:1024	a context-dependent function	997:1024	a context-dependent function	997:1024	We hypothesized that the collagen N-glycan might have a context-dependent function, specifically, that it could be required to promote procollagen folding only when proteostasis is challenged.
33674390	0	4	theme	proteostatic	65:76	arg1	function					78:85	an essential proteostatic function	52:85	an essential proteostatic function	52:85	Collagen's enigmatic, highly conserved N-glycan has an essential proteostatic function.
33674390	5	5	gly	N-glycosylated	839:852	arg1	type-I					889:894	N-glycosylated versus non-N-glycosylated collagen type-I	839:894	N-glycosylated versus non-N-glycosylated collagen type-I	839:894	Surprisingly, however, there is no difference in the folding and secretion of N-glycosylated versus non-N-glycosylated collagen type-I, leaving the function of the N-glycan unclear.
33674390	7	6	theme	ER	1259:1260	arg1	defects					1275:1281	serious procollagen and ER proteostasis defects	1235:1281	defects	1275:1281	We show that removal of the N-glycan from misfolding-prone C-Pro domain variants does indeed cause serious procollagen and ER proteostasis defects.
33674390	1	7	theme	chain	254:258	arg1	register					260:267	chain register	254:267	chain register	254:267	Intracellular procollagen folding begins at the protein's C-terminal propeptide (C-Pro) domain, which initiates triple-helix assembly and defines the composition and chain register of fibrillar collagen trimers.
33674390	9	8	theme	procollagen	1535:1545	arg1	secretion					1507:1515	secretion	1507:1515	secretion	1507:1515	Finally, we show that the C-Pro N-glycan is actually critical for the folding and secretion of even wild-type procollagen under ER stress conditions.
33674390	9	8	theme	procollagen	1535:1545	arg1	folding					1495:1501	folding	1495:1501	folding	1495:1501	Finally, we show that the C-Pro N-glycan is actually critical for the folding and secretion of even wild-type procollagen under ER stress conditions.
33674390	0	9	contain	has	48:50	arg2	function					78:85	an essential proteostatic function	52:85	an essential proteostatic function	52:85	Collagen's enigmatic, highly conserved N-glycan has an essential proteostatic function.
33674390	0	9	contain	has	48:50	arg1	N-glycan					39:46	Collagen's enigmatic, highly conserved N-glycan	0:46	Collagen's enigmatic, highly conserved N-glycan	0:46	Collagen's enigmatic, highly conserved N-glycan has an essential proteostatic function.
33674390	9	10	theme	stress	1556:1561	arg1	conditions					1563:1572	ER stress conditions	1553:1572	ER stress conditions	1553:1572	Finally, we show that the C-Pro N-glycan is actually critical for the folding and secretion of even wild-type procollagen under ER stress conditions.
33674390	8	11	theme	lectin-based	1391:1402	arg1	machinery					1414:1422	the ER's lectin-based chaperone machinery	1382:1422	the ER's lectin-based chaperone machinery	1382:1422	The N-glycan promotes folding and secretion of destabilized C-Pro variants by providing access to the ER's lectin-based chaperone machinery.
33674390	11	12	theme	essential	1751:1759	arg1	function					1780:1787	an essential, context-dependent function	1748:1787	an essential, context-dependent function for procollagen's previously enigmatic N-glycan	1748:1835	Collectively, these results establish an essential, context-dependent function for procollagen's previously enigmatic N-glycan, wherein the carbohydrate moiety buffers procollagen folding against proteostatic challenge.
33674390	3	13	contain	possesses	484:492	arg1	domain					477:482	The procollagen C-Pro domain	455:482	The procollagen C-Pro domain	455:482	The procollagen C-Pro domain possesses a single N-glycosylation site that is widely conserved in all the fibrillar procollagens across humans and diverse other species.
33674390	3	13	contain	possesses	484:492	arg2	site					519:522	a single N-glycosylation site	494:522	a single N-glycosylation site that is widely conserved in all the fibrillar procollagens across humans and diverse other species	494:621	The procollagen C-Pro domain possesses a single N-glycosylation site that is widely conserved in all the fibrillar procollagens across humans and diverse other species.
33674390	5	14	gly	non-N-glycosylated	861:878	arg1	type-I					889:894	N-glycosylated versus non-N-glycosylated collagen type-I	839:894	N-glycosylated versus non-N-glycosylated collagen type-I	839:894	Surprisingly, however, there is no difference in the folding and secretion of N-glycosylated versus non-N-glycosylated collagen type-I, leaving the function of the N-glycan unclear.
33674390	3	15	theme	N-glycosylation	503:517	arg1	site					519:522	a single N-glycosylation site	494:522	a single N-glycosylation site that is widely conserved in all the fibrillar procollagens across humans and diverse other species	494:621	The procollagen C-Pro domain possesses a single N-glycosylation site that is widely conserved in all the fibrillar procollagens across humans and diverse other species.
33674390	9	16	dep	folding	1495:1501	arg1	the					1491:1493	the	1491:1493	the	1491:1493	Finally, we show that the C-Pro N-glycan is actually critical for the folding and secretion of even wild-type procollagen under ER stress conditions.
33674390	5	17	theme	N-glycosylated	839:852	arg1	type-I					889:894	N-glycosylated versus non-N-glycosylated collagen type-I	839:894	N-glycosylated versus non-N-glycosylated collagen type-I	839:894	Surprisingly, however, there is no difference in the folding and secretion of N-glycosylated versus non-N-glycosylated collagen type-I, leaving the function of the N-glycan unclear.
33674390	7	18	theme	C-Pro	1195:1199	arg1	variants					1208:1215	misfolding-prone C-Pro domain variants	1178:1215	misfolding-prone C-Pro domain variants	1178:1215	We show that removal of the N-glycan from misfolding-prone C-Pro domain variants does indeed cause serious procollagen and ER proteostasis defects.
33674390	7	19	theme	proteostasis	1262:1273	arg1	defects					1275:1281	serious procollagen and ER proteostasis defects	1235:1281	defects	1275:1281	We show that removal of the N-glycan from misfolding-prone C-Pro domain variants does indeed cause serious procollagen and ER proteostasis defects.
33674390	6	20	theme	procollagen	1078:1088	arg1	folding					1090:1096	procollagen folding	1078:1096	procollagen folding	1078:1096	We hypothesized that the collagen N-glycan might have a context-dependent function, specifically, that it could be required to promote procollagen folding only when proteostasis is challenged.
33674390	1	21	theme	C-Pro	169:173	arg1	domain					176:181	the protein's C-terminal propeptide (C-Pro) domain	132:181	the protein's C-terminal propeptide (C-Pro) domain	132:181	Intracellular procollagen folding begins at the protein's C-terminal propeptide (C-Pro) domain, which initiates triple-helix assembly and defines the composition and chain register of fibrillar collagen trimers.
33674390	7	22	theme	domain	1201:1206	arg1	variants					1208:1215	misfolding-prone C-Pro domain variants	1178:1215	misfolding-prone C-Pro domain variants	1178:1215	We show that removal of the N-glycan from misfolding-prone C-Pro domain variants does indeed cause serious procollagen and ER proteostasis defects.
33674390	8	23	theme	C-Pro	1344:1348	arg1	variants					1350:1357	destabilized C-Pro variants	1331:1357	destabilized C-Pro variants	1331:1357	The N-glycan promotes folding and secretion of destabilized C-Pro variants by providing access to the ER's lectin-based chaperone machinery.
33674390	9	24	theme	wild-type	1525:1533	arg1	procollagen					1535:1545	even wild-type procollagen	1520:1545	even wild-type procollagen	1520:1545	Finally, we show that the C-Pro N-glycan is actually critical for the folding and secretion of even wild-type procollagen under ER stress conditions.
33674390	3	25	gly	N-glycosylation	503:517	arg2	site					519:522	a single N-glycosylation site	494:522	a single N-glycosylation site that is widely conserved in all the fibrillar procollagens across humans and diverse other species	494:621	The procollagen C-Pro domain possesses a single N-glycosylation site that is widely conserved in all the fibrillar procollagens across humans and diverse other species.
33674390	10	26	theme	Such	1575:1578	arg1	stress					1580:1585	Such stress	1575:1585	Such stress	1575:1585	Such stress is commonly incurred during development, wound healing, and other processes in which collagen production plays a key role.
33674390	10	27	theme	wound	1628:1632	arg1	healing					1634:1640	wound healing	1628:1640	wound healing	1628:1640	Such stress is commonly incurred during development, wound healing, and other processes in which collagen production plays a key role.
33674390	5	28	from	difference	796:805	arg1	secretion					826:834	secretion	826:834	secretion	826:834	Surprisingly, however, there is no difference in the folding and secretion of N-glycosylated versus non-N-glycosylated collagen type-I, leaving the function of the N-glycan unclear.
33674390	5	28	from	difference	796:805	arg1	folding					814:820	folding	814:820	folding	814:820	Surprisingly, however, there is no difference in the folding and secretion of N-glycosylated versus non-N-glycosylated collagen type-I, leaving the function of the N-glycan unclear.
33674390	7	29	theme	serious	1235:1241	arg1	procollagen					1243:1253	serious procollagen and ER proteostasis defects	1235:1281	procollagen	1243:1253	We show that removal of the N-glycan from misfolding-prone C-Pro domain variants does indeed cause serious procollagen and ER proteostasis defects.
33674390	5	30	theme	unclear	934:940	arg1	function					909:916	the function	905:916	the function of the N-glycan unclear	905:940	Surprisingly, however, there is no difference in the folding and secretion of N-glycosylated versus non-N-glycosylated collagen type-I, leaving the function of the N-glycan unclear.
33674390	5	31	theme	non-N-glycosylated	861:878	arg1	type-I					889:894	N-glycosylated versus non-N-glycosylated collagen type-I	839:894	N-glycosylated versus non-N-glycosylated collagen type-I	839:894	Surprisingly, however, there is no difference in the folding and secretion of N-glycosylated versus non-N-glycosylated collagen type-I, leaving the function of the N-glycan unclear.
33674390	2	32	theme	extracellular	433:445	arg1	matrix					447:452	the extracellular matrix	429:452	the extracellular matrix	429:452	The C-Pro domain is later proteolytically cleaved and excreted from the body, while the mature triple helix is incorporated into the extracellular matrix.
33674390	1	33	theme	fibrillar	272:280	arg1	trimers					291:297	fibrillar collagen trimers	272:297	fibrillar collagen trimers	272:297	Intracellular procollagen folding begins at the protein's C-terminal propeptide (C-Pro) domain, which initiates triple-helix assembly and defines the composition and chain register of fibrillar collagen trimers.
33674390	1	34	theme	Intracellular	88:100	arg1	folding					114:120	Intracellular procollagen folding	88:120	Intracellular procollagen folding	88:120	Intracellular procollagen folding begins at the protein's C-terminal propeptide (C-Pro) domain, which initiates triple-helix assembly and defines the composition and chain register of fibrillar collagen trimers.
33674390	4	35	theme	procollagen	668:678	arg1	folding					680:686	procollagen folding	668:686	procollagen folding	668:686	Given that the C-Pro domain is removed once procollagen folding is complete, the N-glycan might be presumed to be important for folding.
33674390	1	36	theme	collagen	282:289	arg1	trimers					291:297	fibrillar collagen trimers	272:297	fibrillar collagen trimers	272:297	Intracellular procollagen folding begins at the protein's C-terminal propeptide (C-Pro) domain, which initiates triple-helix assembly and defines the composition and chain register of fibrillar collagen trimers.
33674390	9	37	theme	C-Pro	1451:1455	arg1	N-glycan					1457:1464	the C-Pro N-glycan	1447:1464	the C-Pro N-glycan	1447:1464	Finally, we show that the C-Pro N-glycan is actually critical for the folding and secretion of even wild-type procollagen under ER stress conditions.
33674390	9	37	theme	C-Pro	1451:1455	arg1	critical					1478:1485	critical	1478:1485	critical	1478:1485	Finally, we show that the C-Pro N-glycan is actually critical for the folding and secretion of even wild-type procollagen under ER stress conditions.
33674390	2	38	theme	C-Pro	304:308	arg1	domain					310:315	The C-Pro domain	300:315	The C-Pro domain	300:315	The C-Pro domain is later proteolytically cleaved and excreted from the body, while the mature triple helix is incorporated into the extracellular matrix.
33674390	3	39	theme	procollagen	459:469	arg1	domain					477:482	The procollagen C-Pro domain	455:482	The procollagen C-Pro domain	455:482	The procollagen C-Pro domain possesses a single N-glycosylation site that is widely conserved in all the fibrillar procollagens across humans and diverse other species.
33674390	0	40	theme	enigmatic	11:19	arg1	N-glycan					39:46	Collagen's enigmatic, highly conserved N-glycan	0:46	Collagen's enigmatic, highly conserved N-glycan	0:46	Collagen's enigmatic, highly conserved N-glycan has an essential proteostatic function.
33674390	1	41	theme	trimers	291:297	arg1	composition					238:248	composition	238:248	composition	238:248	Intracellular procollagen folding begins at the protein's C-terminal propeptide (C-Pro) domain, which initiates triple-helix assembly and defines the composition and chain register of fibrillar collagen trimers.
33674390	1	41	theme	trimers	291:297	arg1	register					260:267	chain register	254:267	chain register	254:267	Intracellular procollagen folding begins at the protein's C-terminal propeptide (C-Pro) domain, which initiates triple-helix assembly and defines the composition and chain register of fibrillar collagen trimers.
33674390	11	42	dep	essential	1751:1759	arg1	context-dependent					1762:1778	context-dependent	1762:1778	context-dependent	1762:1778	Collectively, these results establish an essential, context-dependent function for procollagen's previously enigmatic N-glycan, wherein the carbohydrate moiety buffers procollagen folding against proteostatic challenge.
33674390	3	43	theme	fibrillar	560:568	arg1	procollagens					570:581	all the fibrillar procollagens	552:581	all the fibrillar procollagens across humans and diverse other species	552:621	The procollagen C-Pro domain possesses a single N-glycosylation site that is widely conserved in all the fibrillar procollagens across humans and diverse other species.
33674390	9	44	theme	ER	1553:1554	arg1	conditions					1563:1572	ER stress conditions	1553:1572	ER stress conditions	1553:1572	Finally, we show that the C-Pro N-glycan is actually critical for the folding and secretion of even wild-type procollagen under ER stress conditions.
33674390	3	45	theme	C-Pro	471:475	arg1	domain					477:482	The procollagen C-Pro domain	455:482	The procollagen C-Pro domain	455:482	The procollagen C-Pro domain possesses a single N-glycosylation site that is widely conserved in all the fibrillar procollagens across humans and diverse other species.
33674390	10	46	theme	other	1647:1651	arg1	processes					1653:1661	other processes	1647:1661	other processes in which collagen production plays a key role	1647:1707	Such stress is commonly incurred during development, wound healing, and other processes in which collagen production plays a key role.
33674390	11	47	theme	proteostatic	1906:1917	arg1	challenge					1919:1927	proteostatic challenge	1906:1927	proteostatic challenge	1906:1927	Collectively, these results establish an essential, context-dependent function for procollagen's previously enigmatic N-glycan, wherein the carbohydrate moiety buffers procollagen folding against proteostatic challenge.
33674390	10	48	theme	key	1700:1702	arg1	role					1704:1707	a key role	1698:1707	a key role	1698:1707	Such stress is commonly incurred during development, wound healing, and other processes in which collagen production plays a key role.
33674390	1	49	dep	composition	238:248	arg1	the					234:236	the	234:236	the	234:236	Intracellular procollagen folding begins at the protein's C-terminal propeptide (C-Pro) domain, which initiates triple-helix assembly and defines the composition and chain register of fibrillar collagen trimers.
33674390	3	50	theme	diverse	601:607	arg1	species					615:621	diverse other species	601:621	diverse other species	601:621	The procollagen C-Pro domain possesses a single N-glycosylation site that is widely conserved in all the fibrillar procollagens across humans and diverse other species.
33674390	5	51	theme	collagen	880:887	arg1	type-I					889:894	N-glycosylated versus non-N-glycosylated collagen type-I	839:894	N-glycosylated versus non-N-glycosylated collagen type-I	839:894	Surprisingly, however, there is no difference in the folding and secretion of N-glycosylated versus non-N-glycosylated collagen type-I, leaving the function of the N-glycan unclear.
33674390	1	52	theme	triple-helix	200:211	arg1	assembly					213:220	triple-helix assembly	200:220	triple-helix assembly	200:220	Intracellular procollagen folding begins at the protein's C-terminal propeptide (C-Pro) domain, which initiates triple-helix assembly and defines the composition and chain register of fibrillar collagen trimers.
33674390	2	53	theme	mature	388:393	arg1	helix					402:406	the mature triple helix	384:406	the mature triple helix	384:406	The C-Pro domain is later proteolytically cleaved and excreted from the body, while the mature triple helix is incorporated into the extracellular matrix.
33674390	7	54	from	variants	1208:1215	arg1	removal					1149:1155	removal	1149:1155	removal of the N-glycan from misfolding-prone C-Pro domain variants	1149:1215	We show that removal of the N-glycan from misfolding-prone C-Pro domain variants does indeed cause serious procollagen and ER proteostasis defects.
33674390	3	55	theme	other	609:613	arg1	species					615:621	diverse other species	601:621	diverse other species	601:621	The procollagen C-Pro domain possesses a single N-glycosylation site that is widely conserved in all the fibrillar procollagens across humans and diverse other species.
33674390	2	56	theme	triple	395:400	arg1	helix					402:406	the mature triple helix	384:406	the mature triple helix	384:406	The C-Pro domain is later proteolytically cleaved and excreted from the body, while the mature triple helix is incorporated into the extracellular matrix.
33674390	11	57	dep	moiety	1863:1868	arg1	buffers					1870:1876	buffers	1870:1876	buffers procollagen folding against proteostatic challenge	1870:1927	Collectively, these results establish an essential, context-dependent function for procollagen's previously enigmatic N-glycan, wherein the carbohydrate moiety buffers procollagen folding against proteostatic challenge.
33674390	6	58	theme	collagen	968:975	arg1	N-glycan					977:984	the collagen N-glycan	964:984	the collagen N-glycan	964:984	We hypothesized that the collagen N-glycan might have a context-dependent function, specifically, that it could be required to promote procollagen folding only when proteostasis is challenged.
33674390	6	59	contain	have	992:995	arg2	function					1017:1024	a context-dependent function	997:1024	a context-dependent function	997:1024	We hypothesized that the collagen N-glycan might have a context-dependent function, specifically, that it could be required to promote procollagen folding only when proteostasis is challenged.
33674390	6	59	contain	have	992:995	arg1	N-glycan					977:984	the collagen N-glycan	964:984	the collagen N-glycan	964:984	We hypothesized that the collagen N-glycan might have a context-dependent function, specifically, that it could be required to promote procollagen folding only when proteostasis is challenged.
33674390	3	60	theme	single	496:501	arg1	site					519:522	a single N-glycosylation site	494:522	a single N-glycosylation site that is widely conserved in all the fibrillar procollagens across humans and diverse other species	494:621	The procollagen C-Pro domain possesses a single N-glycosylation site that is widely conserved in all the fibrillar procollagens across humans and diverse other species.
33674390	11	61	theme	carbohydrate	1850:1861	arg1	moiety					1863:1868	the carbohydrate moiety	1846:1868	the carbohydrate moiety buffers procollagen folding against proteostatic challenge	1846:1927	Collectively, these results establish an essential, context-dependent function for procollagen's previously enigmatic N-glycan, wherein the carbohydrate moiety buffers procollagen folding against proteostatic challenge.
33674390	11	62	theme	procollagen	1878:1888	arg1	folding					1890:1896	procollagen folding	1878:1896	procollagen folding	1878:1896	Collectively, these results establish an essential, context-dependent function for procollagen's previously enigmatic N-glycan, wherein the carbohydrate moiety buffers procollagen folding against proteostatic challenge.
33674390	1	63	theme	procollagen	102:112	arg1	folding					114:120	Intracellular procollagen folding	88:120	Intracellular procollagen folding	88:120	Intracellular procollagen folding begins at the protein's C-terminal propeptide (C-Pro) domain, which initiates triple-helix assembly and defines the composition and chain register of fibrillar collagen trimers.
33674390	0	64	dep	enigmatic	11:19	arg1	conserved					29:37	conserved	29:37	conserved	29:37	Collagen's enigmatic, highly conserved N-glycan has an essential proteostatic function.
33674390	8	65	theme	destabilized	1331:1342	arg1	variants					1350:1357	destabilized C-Pro variants	1331:1357	destabilized C-Pro variants	1331:1357	The N-glycan promotes folding and secretion of destabilized C-Pro variants by providing access to the ER's lectin-based chaperone machinery.
33674390	0	66	theme	essential	55:63	arg1	function					78:85	an essential proteostatic function	52:85	an essential proteostatic function	52:85	Collagen's enigmatic, highly conserved N-glycan has an essential proteostatic function.
33674390	8	67	theme	chaperone	1404:1412	arg1	machinery					1414:1422	the ER's lectin-based chaperone machinery	1382:1422	the ER's lectin-based chaperone machinery	1382:1422	The N-glycan promotes folding and secretion of destabilized C-Pro variants by providing access to the ER's lectin-based chaperone machinery.
33674390	5	68	dep	folding	814:820	arg1	the					810:812	the	810:812	the	810:812	Surprisingly, however, there is no difference in the folding and secretion of N-glycosylated versus non-N-glycosylated collagen type-I, leaving the function of the N-glycan unclear.
33674390	4	69	theme	C-Pro	639:643	arg1	domain					645:650	the C-Pro domain	635:650	the C-Pro domain	635:650	Given that the C-Pro domain is removed once procollagen folding is complete, the N-glycan might be presumed to be important for folding.
33674390	5	70	theme	type-I	889:894	arg1	secretion					826:834	secretion	826:834	secretion	826:834	Surprisingly, however, there is no difference in the folding and secretion of N-glycosylated versus non-N-glycosylated collagen type-I, leaving the function of the N-glycan unclear.
33674390	5	70	theme	type-I	889:894	arg1	folding					814:820	folding	814:820	folding	814:820	Surprisingly, however, there is no difference in the folding and secretion of N-glycosylated versus non-N-glycosylated collagen type-I, leaving the function of the N-glycan unclear.
33674390	10	71	theme	collagen	1672:1679	arg1	production					1681:1690	collagen production	1672:1690	collagen production	1672:1690	Such stress is commonly incurred during development, wound healing, and other processes in which collagen production plays a key role.
33674390	11	72	dep	establish	1738:1746	arg1	wherein					1838:1844	wherein	1838:1844	wherein the carbohydrate moiety buffers procollagen folding against proteostatic challenge	1838:1927	Collectively, these results establish an essential, context-dependent function for procollagen's previously enigmatic N-glycan, wherein the carbohydrate moiety buffers procollagen folding against proteostatic challenge.
33674390	7	73	theme	N-glycan	1164:1171	arg1	removal					1149:1155	removal	1149:1155	removal of the N-glycan from misfolding-prone C-Pro domain variants	1149:1215	We show that removal of the N-glycan from misfolding-prone C-Pro domain variants does indeed cause serious procollagen and ER proteostasis defects.
33674390	1	74	theme	C-terminal	146:155	arg1	domain					176:181	the protein's C-terminal propeptide (C-Pro) domain	132:181	the protein's C-terminal propeptide (C-Pro) domain	132:181	Intracellular procollagen folding begins at the protein's C-terminal propeptide (C-Pro) domain, which initiates triple-helix assembly and defines the composition and chain register of fibrillar collagen trimers.
33674390	7	75	theme	misfolding-prone	1178:1193	arg1	variants					1208:1215	misfolding-prone C-Pro domain variants	1178:1215	misfolding-prone C-Pro domain variants	1178:1215	We show that removal of the N-glycan from misfolding-prone C-Pro domain variants does indeed cause serious procollagen and ER proteostasis defects.
33641986	0	0	theme	short-chain	95:105	arg1	acids					113:117	short-chain fatty acids	95:117	short-chain fatty acids	95:117	Cyclocarya paliurus polysaccharide improves metabolic function of gut microbiota by regulating short-chain fatty acids and gut microbiota composition.
33641986	7	1	theme	microbiota	1455:1464	arg1	composition					1436:1446	the composition	1432:1446	the composition of gut microbiota	1432:1464	These findings suggested that early CP intervention could enhance the metabolic function of gut microbiota by increasing the release of SCFAs and altering the composition of gut microbiota.
33641986	4	2	theme	bacteria	819:826	arg1	abundance					797:805	the relative abundance	784:805	the relative abundance of specific bacteria	784:826	16S rRNA showed that 200 mg/kg body weight CP was effective in increasing diversity of the gut microbiota in healthy mice and affected the relative abundance of specific bacteria.
33641986	3	3	theme	short-chain	499:509	arg1	acids					517:521	the short-chain fatty acids	495:521	the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid)	495:590	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	7	4	theme	CP	1313:1314	arg1	intervention					1316:1327	early CP intervention	1307:1327	early CP intervention	1307:1327	These findings suggested that early CP intervention could enhance the metabolic function of gut microbiota by increasing the release of SCFAs and altering the composition of gut microbiota.
33641986	4	5	theme	specific	810:817	arg1	bacteria					819:826	specific bacteria	810:826	specific bacteria	810:826	16S rRNA showed that 200 mg/kg body weight CP was effective in increasing diversity of the gut microbiota in healthy mice and affected the relative abundance of specific bacteria.
33641986	1	6	theme	paper	171:175	arg1	purpose					155:161	The purpose	151:161	The purpose of this paper	151:175	The purpose of this paper was to investigate the effects of Cyclocarya paliurus polysaccharide (CP) on gut microbiota composition and predict metabolic function in healthy mice.
33641986	1	7	theme	gut	254:256	arg1	composition					269:279	gut microbiota composition	254:279	gut microbiota composition	254:279	The purpose of this paper was to investigate the effects of Cyclocarya paliurus polysaccharide (CP) on gut microbiota composition and predict metabolic function in healthy mice.
33641986	6	8	theme	amino	1044:1048	arg1	metabolism					1080:1089	glycerolipid metabolism	1067:1089	glycerolipid metabolism	1067:1089	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	8	theme	amino	1044:1048	arg1	biosynthesis					1127:1138	biosynthesis	1127:1138	biosynthesis of unsaturated fatty acids	1127:1165	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	8	theme	amino	1044:1048	arg1	metabolism					1055:1064	amino acid metabolism	1044:1064	amino acid metabolism	1044:1064	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	8	theme	amino	1044:1048	arg1	metabolism					1197:1206	C5-branched dibasic acid metabolism	1172:1206	C5-branched dibasic acid metabolism	1172:1206	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	8	theme	amino	1044:1048	arg1	biosynthesis					1113:1124	pantothenate and CoA biosynthesis	1092:1124	biosynthesis	1113:1124	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	8	theme	amino	1044:1048	arg1	pathways					1249:1256	the primary enriched phenotypic KEGG pathways	1212:1256	the primary enriched phenotypic KEGG pathways in the CP-H group	1212:1274	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	8	theme	amino	1044:1048	arg1	metabolism					1032:1041	starch and sucrose metabolism	1013:1041	metabolism	1032:1041	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	9	theme	KEGG	1244:1247	arg1	metabolism					1080:1089	glycerolipid metabolism	1067:1089	glycerolipid metabolism	1067:1089	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	9	theme	KEGG	1244:1247	arg1	biosynthesis					1127:1138	biosynthesis	1127:1138	biosynthesis of unsaturated fatty acids	1127:1165	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	9	theme	KEGG	1244:1247	arg1	metabolism					1055:1064	amino acid metabolism	1044:1064	amino acid metabolism	1044:1064	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	9	theme	KEGG	1244:1247	arg1	metabolism					1197:1206	C5-branched dibasic acid metabolism	1172:1206	C5-branched dibasic acid metabolism	1172:1206	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	9	theme	KEGG	1244:1247	arg1	biosynthesis					1113:1124	pantothenate and CoA biosynthesis	1092:1124	biosynthesis	1113:1124	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	9	theme	KEGG	1244:1247	arg1	pathways					1249:1256	the primary enriched phenotypic KEGG pathways	1212:1256	the primary enriched phenotypic KEGG pathways in the CP-H group	1212:1274	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	9	theme	KEGG	1244:1247	arg1	metabolism					1032:1041	starch and sucrose metabolism	1013:1041	metabolism	1032:1041	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	4	10	from	diversity	723:731	arg1	mice					766:769	healthy mice	758:769	healthy mice	758:769	16S rRNA showed that 200 mg/kg body weight CP was effective in increasing diversity of the gut microbiota in healthy mice and affected the relative abundance of specific bacteria.
33641986	3	11	theme	acetic	531:536	arg1	SCFAs					524:528	SCFAs	524:528	SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid	524:589	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	3	11	theme	acetic	531:536	arg1	acid					538:541	acetic acid	531:541	acetic acid	531:541	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	3	12	theme	propionic	544:552	arg1	SCFAs					524:528	SCFAs	524:528	SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid	524:589	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	3	12	theme	propionic	544:552	arg1	acid					554:557	propionic acid	544:557	propionic acid	544:557	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	1	13	theme	microbiota	258:267	arg1	composition					269:279	gut microbiota composition	254:279	gut microbiota composition	254:279	The purpose of this paper was to investigate the effects of Cyclocarya paliurus polysaccharide (CP) on gut microbiota composition and predict metabolic function in healthy mice.
33641986	0	14	theme	fatty	107:111	arg1	acids					113:117	short-chain fatty acids	95:117	short-chain fatty acids	95:117	Cyclocarya paliurus polysaccharide improves metabolic function of gut microbiota by regulating short-chain fatty acids and gut microbiota composition.
33641986	6	15	theme	phenotypic	1233:1242	arg1	metabolism					1080:1089	glycerolipid metabolism	1067:1089	glycerolipid metabolism	1067:1089	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	15	theme	phenotypic	1233:1242	arg1	biosynthesis					1127:1138	biosynthesis	1127:1138	biosynthesis of unsaturated fatty acids	1127:1165	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	15	theme	phenotypic	1233:1242	arg1	metabolism					1055:1064	amino acid metabolism	1044:1064	amino acid metabolism	1044:1064	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	15	theme	phenotypic	1233:1242	arg1	metabolism					1197:1206	C5-branched dibasic acid metabolism	1172:1206	C5-branched dibasic acid metabolism	1172:1206	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	15	theme	phenotypic	1233:1242	arg1	biosynthesis					1113:1124	pantothenate and CoA biosynthesis	1092:1124	biosynthesis	1113:1124	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	15	theme	phenotypic	1233:1242	arg1	pathways					1249:1256	the primary enriched phenotypic KEGG pathways	1212:1256	the primary enriched phenotypic KEGG pathways in the CP-H group	1212:1274	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	15	theme	phenotypic	1233:1242	arg1	metabolism					1032:1041	starch and sucrose metabolism	1013:1041	metabolism	1032:1041	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	5	16	theme	CP-H	928:931	arg1	group					933:937	the CP-H group	924:937	the CP-H group	924:937	Lachnospiraceae, Clostridiales, and Clostridia were identified as the phenotypic biomarkers of the CP-H group compared with the normal control group.
33641986	4	17	theme	200 mg/kg	670:678	arg1	weight					685:690	200 mg/kg body weight	670:690	200 mg/kg body weight CP	670:693	16S rRNA showed that 200 mg/kg body weight CP was effective in increasing diversity of the gut microbiota in healthy mice and affected the relative abundance of specific bacteria.
33641986	0	18	theme	gut	123:125	arg1	composition					138:148	gut microbiota composition	123:148	gut microbiota composition	123:148	Cyclocarya paliurus polysaccharide improves metabolic function of gut microbiota by regulating short-chain fatty acids and gut microbiota composition.
33641986	4	19	theme	relative	788:795	arg1	abundance					797:805	the relative abundance	784:805	the relative abundance of specific bacteria	784:826	16S rRNA showed that 200 mg/kg body weight CP was effective in increasing diversity of the gut microbiota in healthy mice and affected the relative abundance of specific bacteria.
33641986	5	20	theme	group	933:937	arg1	biomarkers					910:919	the phenotypic biomarkers	895:919	the phenotypic biomarkers of the CP-H group	895:937	Lachnospiraceae, Clostridiales, and Clostridia were identified as the phenotypic biomarkers of the CP-H group compared with the normal control group.
33641986	5	20	theme	group	933:937	arg1	Clostridiales					846:858	Clostridiales	846:858	Clostridiales	846:858	Lachnospiraceae, Clostridiales, and Clostridia were identified as the phenotypic biomarkers of the CP-H group compared with the normal control group.
33641986	5	20	theme	group	933:937	arg1	Clostridia					865:874	Clostridia	865:874	Clostridia	865:874	Lachnospiraceae, Clostridiales, and Clostridia were identified as the phenotypic biomarkers of the CP-H group compared with the normal control group.
33641986	5	20	theme	group	933:937	arg1	Lachnospiraceae					829:843	Lachnospiraceae	829:843	Lachnospiraceae	829:843	Lachnospiraceae, Clostridiales, and Clostridia were identified as the phenotypic biomarkers of the CP-H group compared with the normal control group.
33641986	7	21	theme	early	1307:1311	arg1	intervention					1316:1327	early CP intervention	1307:1327	early CP intervention	1307:1327	These findings suggested that early CP intervention could enhance the metabolic function of gut microbiota by increasing the release of SCFAs and altering the composition of gut microbiota.
33641986	7	22	theme	SCFAs	1413:1417	arg1	release					1402:1408	the release	1398:1408	the release of SCFAs	1398:1417	These findings suggested that early CP intervention could enhance the metabolic function of gut microbiota by increasing the release of SCFAs and altering the composition of gut microbiota.
33641986	1	23	from	function	303:310	arg1	mice					323:326	healthy mice	315:326	healthy mice	315:326	The purpose of this paper was to investigate the effects of Cyclocarya paliurus polysaccharide (CP) on gut microbiota composition and predict metabolic function in healthy mice.
33641986	6	24	theme	sucrose	1024:1030	arg1	metabolism					1080:1089	glycerolipid metabolism	1067:1089	glycerolipid metabolism	1067:1089	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	24	theme	sucrose	1024:1030	arg1	biosynthesis					1127:1138	biosynthesis	1127:1138	biosynthesis of unsaturated fatty acids	1127:1165	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	24	theme	sucrose	1024:1030	arg1	metabolism					1055:1064	amino acid metabolism	1044:1064	amino acid metabolism	1044:1064	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	24	theme	sucrose	1024:1030	arg1	metabolism					1197:1206	C5-branched dibasic acid metabolism	1172:1206	C5-branched dibasic acid metabolism	1172:1206	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	24	theme	sucrose	1024:1030	arg1	biosynthesis					1113:1124	pantothenate and CoA biosynthesis	1092:1124	biosynthesis	1113:1124	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	24	theme	sucrose	1024:1030	arg1	pathways					1249:1256	the primary enriched phenotypic KEGG pathways	1212:1256	the primary enriched phenotypic KEGG pathways in the CP-H group	1212:1274	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	24	theme	sucrose	1024:1030	arg1	metabolism					1032:1041	starch and sucrose metabolism	1013:1041	metabolism	1032:1041	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	0	25	theme	paliurus	11:18	arg1	polysaccharide					20:33	Cyclocarya paliurus polysaccharide	0:33	Cyclocarya paliurus polysaccharide	0:33	Cyclocarya paliurus polysaccharide improves metabolic function of gut microbiota by regulating short-chain fatty acids and gut microbiota composition.
33641986	6	26	theme	enriched	1224:1231	arg1	metabolism					1080:1089	glycerolipid metabolism	1067:1089	glycerolipid metabolism	1067:1089	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	26	theme	enriched	1224:1231	arg1	biosynthesis					1127:1138	biosynthesis	1127:1138	biosynthesis of unsaturated fatty acids	1127:1165	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	26	theme	enriched	1224:1231	arg1	metabolism					1055:1064	amino acid metabolism	1044:1064	amino acid metabolism	1044:1064	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	26	theme	enriched	1224:1231	arg1	metabolism					1197:1206	C5-branched dibasic acid metabolism	1172:1206	C5-branched dibasic acid metabolism	1172:1206	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	26	theme	enriched	1224:1231	arg1	biosynthesis					1113:1124	pantothenate and CoA biosynthesis	1092:1124	biosynthesis	1113:1124	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	26	theme	enriched	1224:1231	arg1	pathways					1249:1256	the primary enriched phenotypic KEGG pathways	1212:1256	the primary enriched phenotypic KEGG pathways in the CP-H group	1212:1274	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	26	theme	enriched	1224:1231	arg1	metabolism					1032:1041	starch and sucrose metabolism	1013:1041	metabolism	1032:1041	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	0	27	theme	Cyclocarya	0:9	arg1	polysaccharide					20:33	Cyclocarya paliurus polysaccharide	0:33	Cyclocarya paliurus polysaccharide	0:33	Cyclocarya paliurus polysaccharide improves metabolic function of gut microbiota by regulating short-chain fatty acids and gut microbiota composition.
33641986	6	28	theme	acids	1161:1165	arg1	metabolism					1080:1089	glycerolipid metabolism	1067:1089	glycerolipid metabolism	1067:1089	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	28	theme	acids	1161:1165	arg1	biosynthesis					1127:1138	biosynthesis	1127:1138	biosynthesis of unsaturated fatty acids	1127:1165	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	28	theme	acids	1161:1165	arg1	metabolism					1055:1064	amino acid metabolism	1044:1064	amino acid metabolism	1044:1064	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	28	theme	acids	1161:1165	arg1	metabolism					1197:1206	C5-branched dibasic acid metabolism	1172:1206	C5-branched dibasic acid metabolism	1172:1206	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	28	theme	acids	1161:1165	arg1	biosynthesis					1113:1124	pantothenate and CoA biosynthesis	1092:1124	biosynthesis	1113:1124	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	28	theme	acids	1161:1165	arg1	pathways					1249:1256	the primary enriched phenotypic KEGG pathways	1212:1256	the primary enriched phenotypic KEGG pathways in the CP-H group	1212:1274	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	28	theme	acids	1161:1165	arg1	metabolism					1032:1041	starch and sucrose metabolism	1013:1041	metabolism	1032:1041	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	4	29	theme	weight	685:690	arg1	CP					692:693	200 mg/kg body weight CP	670:693	200 mg/kg body weight CP	670:693	16S rRNA showed that 200 mg/kg body weight CP was effective in increasing diversity of the gut microbiota in healthy mice and affected the relative abundance of specific bacteria.
33641986	4	30	theme	16S	649:651	arg1	rRNA					653:656	16S rRNA	649:656	16S rRNA	649:656	16S rRNA showed that 200 mg/kg body weight CP was effective in increasing diversity of the gut microbiota in healthy mice and affected the relative abundance of specific bacteria.
33641986	5	31	theme	normal	957:962	arg1	group					972:976	the normal control group	953:976	the normal control group	953:976	Lachnospiraceae, Clostridiales, and Clostridia were identified as the phenotypic biomarkers of the CP-H group compared with the normal control group.
33641986	6	32	theme	C5-branched	1172:1182	arg1	metabolism					1080:1089	glycerolipid metabolism	1067:1089	glycerolipid metabolism	1067:1089	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	32	theme	C5-branched	1172:1182	arg1	biosynthesis					1127:1138	biosynthesis	1127:1138	biosynthesis of unsaturated fatty acids	1127:1165	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	32	theme	C5-branched	1172:1182	arg1	metabolism					1055:1064	amino acid metabolism	1044:1064	amino acid metabolism	1044:1064	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	32	theme	C5-branched	1172:1182	arg1	metabolism					1197:1206	C5-branched dibasic acid metabolism	1172:1206	C5-branched dibasic acid metabolism	1172:1206	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	32	theme	C5-branched	1172:1182	arg1	biosynthesis					1113:1124	pantothenate and CoA biosynthesis	1092:1124	biosynthesis	1113:1124	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	32	theme	C5-branched	1172:1182	arg1	pathways					1249:1256	the primary enriched phenotypic KEGG pathways	1212:1256	the primary enriched phenotypic KEGG pathways in the CP-H group	1212:1274	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	32	theme	C5-branched	1172:1182	arg1	metabolism					1032:1041	starch and sucrose metabolism	1013:1041	metabolism	1032:1041	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	3	33	dep	acids	517:521	arg1	acid					586:589	valeric acid	578:589	valeric acid	578:589	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	3	33	dep	acids	517:521	arg1	SCFAs					524:528	SCFAs	524:528	SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid	524:589	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	3	33	dep	acids	517:521	arg1	acid					538:541	acetic acid	531:541	acetic acid	531:541	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	3	33	dep	acids	517:521	arg1	acid					554:557	propionic acid	544:557	propionic acid	544:557	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	3	33	dep	acids	517:521	arg1	acid					568:571	butyric acid	560:571	butyric acid	560:571	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	5	34	theme	phenotypic	899:908	arg1	biomarkers					910:919	the phenotypic biomarkers	895:919	the phenotypic biomarkers of the CP-H group	895:937	Lachnospiraceae, Clostridiales, and Clostridia were identified as the phenotypic biomarkers of the CP-H group compared with the normal control group.
33641986	5	34	theme	phenotypic	899:908	arg1	Clostridiales					846:858	Clostridiales	846:858	Clostridiales	846:858	Lachnospiraceae, Clostridiales, and Clostridia were identified as the phenotypic biomarkers of the CP-H group compared with the normal control group.
33641986	5	34	theme	phenotypic	899:908	arg1	Clostridia					865:874	Clostridia	865:874	Clostridia	865:874	Lachnospiraceae, Clostridiales, and Clostridia were identified as the phenotypic biomarkers of the CP-H group compared with the normal control group.
33641986	5	34	theme	phenotypic	899:908	arg1	Lachnospiraceae					829:843	Lachnospiraceae	829:843	Lachnospiraceae	829:843	Lachnospiraceae, Clostridiales, and Clostridia were identified as the phenotypic biomarkers of the CP-H group compared with the normal control group.
33641986	1	35	theme	metabolic	293:301	arg1	function					303:310	metabolic function	293:310	metabolic function in healthy mice	293:326	The purpose of this paper was to investigate the effects of Cyclocarya paliurus polysaccharide (CP) on gut microbiota composition and predict metabolic function in healthy mice.
33641986	0	36	theme	microbiota	127:136	arg1	composition					138:148	gut microbiota composition	123:148	gut microbiota composition	123:148	Cyclocarya paliurus polysaccharide improves metabolic function of gut microbiota by regulating short-chain fatty acids and gut microbiota composition.
33641986	6	37	theme	fatty	1155:1159	arg1	acids					1161:1165	unsaturated fatty acids	1143:1165	unsaturated fatty acids	1143:1165	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	3	38	theme	fatty	511:515	arg1	acids					517:521	the short-chain fatty acids	495:521	the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid)	495:590	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	6	39	theme	CoA	1109:1111	arg1	metabolism					1080:1089	glycerolipid metabolism	1067:1089	glycerolipid metabolism	1067:1089	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	39	theme	CoA	1109:1111	arg1	biosynthesis					1127:1138	biosynthesis	1127:1138	biosynthesis of unsaturated fatty acids	1127:1165	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	39	theme	CoA	1109:1111	arg1	metabolism					1055:1064	amino acid metabolism	1044:1064	amino acid metabolism	1044:1064	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	39	theme	CoA	1109:1111	arg1	metabolism					1197:1206	C5-branched dibasic acid metabolism	1172:1206	C5-branched dibasic acid metabolism	1172:1206	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	39	theme	CoA	1109:1111	arg1	biosynthesis					1113:1124	pantothenate and CoA biosynthesis	1092:1124	biosynthesis	1113:1124	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	39	theme	CoA	1109:1111	arg1	pathways					1249:1256	the primary enriched phenotypic KEGG pathways	1212:1256	the primary enriched phenotypic KEGG pathways in the CP-H group	1212:1274	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	39	theme	CoA	1109:1111	arg1	metabolism					1032:1041	starch and sucrose metabolism	1013:1041	metabolism	1032:1041	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	40	theme	dibasic	1184:1190	arg1	metabolism					1080:1089	glycerolipid metabolism	1067:1089	glycerolipid metabolism	1067:1089	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	40	theme	dibasic	1184:1190	arg1	biosynthesis					1127:1138	biosynthesis	1127:1138	biosynthesis of unsaturated fatty acids	1127:1165	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	40	theme	dibasic	1184:1190	arg1	metabolism					1055:1064	amino acid metabolism	1044:1064	amino acid metabolism	1044:1064	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	40	theme	dibasic	1184:1190	arg1	metabolism					1197:1206	C5-branched dibasic acid metabolism	1172:1206	C5-branched dibasic acid metabolism	1172:1206	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	40	theme	dibasic	1184:1190	arg1	biosynthesis					1113:1124	pantothenate and CoA biosynthesis	1092:1124	biosynthesis	1113:1124	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	40	theme	dibasic	1184:1190	arg1	pathways					1249:1256	the primary enriched phenotypic KEGG pathways	1212:1256	the primary enriched phenotypic KEGG pathways in the CP-H group	1212:1274	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	40	theme	dibasic	1184:1190	arg1	metabolism					1032:1041	starch and sucrose metabolism	1013:1041	metabolism	1032:1041	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	3	41	theme	healthy	608:614	arg1	mice					616:619	healthy mice	608:619	healthy mice	608:619	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	7	42	theme	metabolic	1347:1355	arg1	function					1357:1364	the metabolic function	1343:1364	the metabolic function of gut microbiota	1343:1382	These findings suggested that early CP intervention could enhance the metabolic function of gut microbiota by increasing the release of SCFAs and altering the composition of gut microbiota.
33641986	6	43	theme	starch	1013:1018	arg1	metabolism					1080:1089	glycerolipid metabolism	1067:1089	glycerolipid metabolism	1067:1089	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	43	theme	starch	1013:1018	arg1	biosynthesis					1127:1138	biosynthesis	1127:1138	biosynthesis of unsaturated fatty acids	1127:1165	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	43	theme	starch	1013:1018	arg1	metabolism					1055:1064	amino acid metabolism	1044:1064	amino acid metabolism	1044:1064	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	43	theme	starch	1013:1018	arg1	metabolism					1197:1206	C5-branched dibasic acid metabolism	1172:1206	C5-branched dibasic acid metabolism	1172:1206	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	43	theme	starch	1013:1018	arg1	biosynthesis					1113:1124	pantothenate and CoA biosynthesis	1092:1124	biosynthesis	1113:1124	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	43	theme	starch	1013:1018	arg1	pathways					1249:1256	the primary enriched phenotypic KEGG pathways	1212:1256	the primary enriched phenotypic KEGG pathways in the CP-H group	1212:1274	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	43	theme	starch	1013:1018	arg1	metabolism					1032:1041	starch and sucrose metabolism	1013:1041	metabolism	1032:1041	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	1	44	theme	Cyclocarya	211:220	arg1	CP					247:248	CP	247:248	CP	247:248	The purpose of this paper was to investigate the effects of Cyclocarya paliurus polysaccharide (CP) on gut microbiota composition and predict metabolic function in healthy mice.
33641986	1	44	theme	Cyclocarya	211:220	arg1	polysaccharide					231:244	Cyclocarya paliurus polysaccharide	211:244	Cyclocarya paliurus polysaccharide (CP)	211:249	The purpose of this paper was to investigate the effects of Cyclocarya paliurus polysaccharide (CP) on gut microbiota composition and predict metabolic function in healthy mice.
33641986	0	45	theme	metabolic	44:52	arg1	function					54:61	metabolic function	44:61	metabolic function of gut microbiota	44:79	Cyclocarya paliurus polysaccharide improves metabolic function of gut microbiota by regulating short-chain fatty acids and gut microbiota composition.
33641986	4	46	theme	healthy	758:764	arg1	mice					766:769	healthy mice	758:769	healthy mice	758:769	16S rRNA showed that 200 mg/kg body weight CP was effective in increasing diversity of the gut microbiota in healthy mice and affected the relative abundance of specific bacteria.
33641986	1	47	theme	paliurus	222:229	arg1	CP					247:248	CP	247:248	CP	247:248	The purpose of this paper was to investigate the effects of Cyclocarya paliurus polysaccharide (CP) on gut microbiota composition and predict metabolic function in healthy mice.
33641986	1	47	theme	paliurus	222:229	arg1	polysaccharide					231:244	Cyclocarya paliurus polysaccharide	211:244	Cyclocarya paliurus polysaccharide (CP)	211:249	The purpose of this paper was to investigate the effects of Cyclocarya paliurus polysaccharide (CP) on gut microbiota composition and predict metabolic function in healthy mice.
33641986	0	48	theme	gut	66:68	arg1	microbiota					70:79	gut microbiota	66:79	gut microbiota	66:79	Cyclocarya paliurus polysaccharide improves metabolic function of gut microbiota by regulating short-chain fatty acids and gut microbiota composition.
33641986	1	49	theme	healthy	315:321	arg1	mice					323:326	healthy mice	315:326	healthy mice	315:326	The purpose of this paper was to investigate the effects of Cyclocarya paliurus polysaccharide (CP) on gut microbiota composition and predict metabolic function in healthy mice.
33641986	3	50	theme	butyric	560:566	arg1	SCFAs					524:528	SCFAs	524:528	SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid	524:589	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	3	50	theme	butyric	560:566	arg1	acid					568:571	butyric acid	560:571	butyric acid	560:571	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	6	51	theme	glycerolipid	1067:1078	arg1	metabolism					1080:1089	glycerolipid metabolism	1067:1089	glycerolipid metabolism	1067:1089	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	51	theme	glycerolipid	1067:1078	arg1	biosynthesis					1127:1138	biosynthesis	1127:1138	biosynthesis of unsaturated fatty acids	1127:1165	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	51	theme	glycerolipid	1067:1078	arg1	metabolism					1055:1064	amino acid metabolism	1044:1064	amino acid metabolism	1044:1064	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	51	theme	glycerolipid	1067:1078	arg1	metabolism					1197:1206	C5-branched dibasic acid metabolism	1172:1206	C5-branched dibasic acid metabolism	1172:1206	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	51	theme	glycerolipid	1067:1078	arg1	biosynthesis					1113:1124	pantothenate and CoA biosynthesis	1092:1124	biosynthesis	1113:1124	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	51	theme	glycerolipid	1067:1078	arg1	pathways					1249:1256	the primary enriched phenotypic KEGG pathways	1212:1256	the primary enriched phenotypic KEGG pathways in the CP-H group	1212:1274	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	51	theme	glycerolipid	1067:1078	arg1	metabolism					1032:1041	starch and sucrose metabolism	1013:1041	metabolism	1032:1041	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	3	52	dep	SCFAs	524:528	arg1	acid					586:589	valeric acid	578:589	valeric acid	578:589	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	3	52	dep	SCFAs	524:528	arg1	SCFAs					524:528	SCFAs	524:528	SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid	524:589	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	3	52	dep	SCFAs	524:528	arg1	acid					538:541	acetic acid	531:541	acetic acid	531:541	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	3	52	dep	SCFAs	524:528	arg1	acid					554:557	propionic acid	544:557	propionic acid	544:557	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	3	52	dep	SCFAs	524:528	arg1	acid					568:571	butyric acid	560:571	butyric acid	560:571	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	7	53	theme	microbiota	1373:1382	arg1	function					1357:1364	the metabolic function	1343:1364	the metabolic function of gut microbiota	1343:1382	These findings suggested that early CP intervention could enhance the metabolic function of gut microbiota by increasing the release of SCFAs and altering the composition of gut microbiota.
33641986	7	54	theme	gut	1451:1453	arg1	microbiota					1455:1464	gut microbiota	1451:1464	gut microbiota	1451:1464	These findings suggested that early CP intervention could enhance the metabolic function of gut microbiota by increasing the release of SCFAs and altering the composition of gut microbiota.
33641986	6	55	theme	primary	1216:1222	arg1	metabolism					1080:1089	glycerolipid metabolism	1067:1089	glycerolipid metabolism	1067:1089	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	55	theme	primary	1216:1222	arg1	biosynthesis					1127:1138	biosynthesis	1127:1138	biosynthesis of unsaturated fatty acids	1127:1165	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	55	theme	primary	1216:1222	arg1	metabolism					1055:1064	amino acid metabolism	1044:1064	amino acid metabolism	1044:1064	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	55	theme	primary	1216:1222	arg1	metabolism					1197:1206	C5-branched dibasic acid metabolism	1172:1206	C5-branched dibasic acid metabolism	1172:1206	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	55	theme	primary	1216:1222	arg1	biosynthesis					1113:1124	pantothenate and CoA biosynthesis	1092:1124	biosynthesis	1113:1124	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	55	theme	primary	1216:1222	arg1	pathways					1249:1256	the primary enriched phenotypic KEGG pathways	1212:1256	the primary enriched phenotypic KEGG pathways in the CP-H group	1212:1274	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	55	theme	primary	1216:1222	arg1	metabolism					1032:1041	starch and sucrose metabolism	1013:1041	metabolism	1032:1041	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	1	56	from	effects	200:206	arg1	composition					269:279	gut microbiota composition	254:279	gut microbiota composition	254:279	The purpose of this paper was to investigate the effects of Cyclocarya paliurus polysaccharide (CP) on gut microbiota composition and predict metabolic function in healthy mice.
33641986	5	57	theme	control	964:970	arg1	group					972:976	the normal control group	953:976	the normal control group	953:976	Lachnospiraceae, Clostridiales, and Clostridia were identified as the phenotypic biomarkers of the CP-H group compared with the normal control group.
33641986	6	58	theme	CP-H	1265:1268	arg1	group					1270:1274	the CP-H group	1261:1274	the CP-H group	1261:1274	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	3	59	theme	dose-dependent	626:639	arg1	matter					641:646	a dose-dependent matter	624:646	a dose-dependent matter	624:646	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	4	60	theme	gut	740:742	arg1	microbiota					744:753	the gut microbiota	736:753	the gut microbiota	736:753	16S rRNA showed that 200 mg/kg body weight CP was effective in increasing diversity of the gut microbiota in healthy mice and affected the relative abundance of specific bacteria.
33641986	6	61	theme	acid	1192:1195	arg1	metabolism					1080:1089	glycerolipid metabolism	1067:1089	glycerolipid metabolism	1067:1089	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	61	theme	acid	1192:1195	arg1	biosynthesis					1127:1138	biosynthesis	1127:1138	biosynthesis of unsaturated fatty acids	1127:1165	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	61	theme	acid	1192:1195	arg1	metabolism					1055:1064	amino acid metabolism	1044:1064	amino acid metabolism	1044:1064	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	61	theme	acid	1192:1195	arg1	metabolism					1197:1206	C5-branched dibasic acid metabolism	1172:1206	C5-branched dibasic acid metabolism	1172:1206	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	61	theme	acid	1192:1195	arg1	biosynthesis					1113:1124	pantothenate and CoA biosynthesis	1092:1124	biosynthesis	1113:1124	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	61	theme	acid	1192:1195	arg1	pathways					1249:1256	the primary enriched phenotypic KEGG pathways	1212:1256	the primary enriched phenotypic KEGG pathways in the CP-H group	1212:1274	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	61	theme	acid	1192:1195	arg1	metabolism					1032:1041	starch and sucrose metabolism	1013:1041	metabolism	1032:1041	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	2	62	theme	Kunming	337:343	arg1	mice					345:348	Healthy Kunming mice	329:348	Healthy Kunming mice	329:348	Healthy Kunming mice were continuously gavaged with CP for 20 days, and mouse feces were collected for analysis.
33641986	1	63	theme	polysaccharide	231:244	arg1	effects					200:206	the effects	196:206	the effects of Cyclocarya paliurus polysaccharide (CP) on gut microbiota composition	196:279	The purpose of this paper was to investigate the effects of Cyclocarya paliurus polysaccharide (CP) on gut microbiota composition and predict metabolic function in healthy mice.
33641986	3	64	theme	mice	616:619	arg1	feces					599:603	the feces	595:603	the feces of healthy mice in a dose-dependent matter	595:646	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	6	65	theme	unsaturated	1143:1153	arg1	acids					1161:1165	unsaturated fatty acids	1143:1165	unsaturated fatty acids	1143:1165	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	2	66	theme	Healthy	329:335	arg1	mice					345:348	Healthy Kunming mice	329:348	Healthy Kunming mice	329:348	Healthy Kunming mice were continuously gavaged with CP for 20 days, and mouse feces were collected for analysis.
33641986	4	67	theme	body	680:683	arg1	weight					685:690	200 mg/kg body weight	670:690	200 mg/kg body weight CP	670:693	16S rRNA showed that 200 mg/kg body weight CP was effective in increasing diversity of the gut microbiota in healthy mice and affected the relative abundance of specific bacteria.
33641986	2	68	theme	mouse	401:405	arg1	feces					407:411	mouse feces	401:411	mouse feces	401:411	Healthy Kunming mice were continuously gavaged with CP for 20 days, and mouse feces were collected for analysis.
33641986	3	69	theme	valeric	578:584	arg1	acid					586:589	valeric acid	578:589	valeric acid	578:589	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	3	69	theme	valeric	578:584	arg1	SCFAs					524:528	SCFAs	524:528	SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid	524:589	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
33641986	6	70	from	pathways	1249:1256	arg1	group					1270:1274	the CP-H group	1261:1274	the CP-H group	1261:1274	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	0	71	theme	microbiota	70:79	arg1	function					54:61	metabolic function	44:61	metabolic function of gut microbiota	44:79	Cyclocarya paliurus polysaccharide improves metabolic function of gut microbiota by regulating short-chain fatty acids and gut microbiota composition.
33641986	6	72	theme	pantothenate	1092:1103	arg1	metabolism					1080:1089	glycerolipid metabolism	1067:1089	glycerolipid metabolism	1067:1089	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	72	theme	pantothenate	1092:1103	arg1	biosynthesis					1127:1138	biosynthesis	1127:1138	biosynthesis of unsaturated fatty acids	1127:1165	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	72	theme	pantothenate	1092:1103	arg1	metabolism					1055:1064	amino acid metabolism	1044:1064	amino acid metabolism	1044:1064	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	72	theme	pantothenate	1092:1103	arg1	metabolism					1197:1206	C5-branched dibasic acid metabolism	1172:1206	C5-branched dibasic acid metabolism	1172:1206	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	72	theme	pantothenate	1092:1103	arg1	biosynthesis					1113:1124	pantothenate and CoA biosynthesis	1092:1124	biosynthesis	1113:1124	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	72	theme	pantothenate	1092:1103	arg1	pathways					1249:1256	the primary enriched phenotypic KEGG pathways	1212:1256	the primary enriched phenotypic KEGG pathways in the CP-H group	1212:1274	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	72	theme	pantothenate	1092:1103	arg1	metabolism					1032:1041	starch and sucrose metabolism	1013:1041	metabolism	1032:1041	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	4	73	theme	microbiota	744:753	arg1	diversity					723:731	diversity	723:731	diversity of the gut microbiota in healthy mice	723:769	16S rRNA showed that 200 mg/kg body weight CP was effective in increasing diversity of the gut microbiota in healthy mice and affected the relative abundance of specific bacteria.
33641986	6	74	theme	acid	1050:1053	arg1	metabolism					1080:1089	glycerolipid metabolism	1067:1089	glycerolipid metabolism	1067:1089	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	74	theme	acid	1050:1053	arg1	biosynthesis					1127:1138	biosynthesis	1127:1138	biosynthesis of unsaturated fatty acids	1127:1165	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	74	theme	acid	1050:1053	arg1	metabolism					1055:1064	amino acid metabolism	1044:1064	amino acid metabolism	1044:1064	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	74	theme	acid	1050:1053	arg1	metabolism					1197:1206	C5-branched dibasic acid metabolism	1172:1206	C5-branched dibasic acid metabolism	1172:1206	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	74	theme	acid	1050:1053	arg1	biosynthesis					1113:1124	pantothenate and CoA biosynthesis	1092:1124	biosynthesis	1113:1124	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	74	theme	acid	1050:1053	arg1	pathways					1249:1256	the primary enriched phenotypic KEGG pathways	1212:1256	the primary enriched phenotypic KEGG pathways in the CP-H group	1212:1274	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	6	74	theme	acid	1050:1053	arg1	metabolism					1032:1041	starch and sucrose metabolism	1013:1041	metabolism	1032:1041	In addition, PICRUSt2 showed that starch and sucrose metabolism, amino acid metabolism, glycerolipid metabolism, pantothenate and CoA biosynthesis, biosynthesis of unsaturated fatty acids, and C5-branched dibasic acid metabolism are the primary enriched phenotypic KEGG pathways in the CP-H group.
33641986	7	75	theme	gut	1369:1371	arg1	microbiota					1373:1382	gut microbiota	1369:1382	gut microbiota	1369:1382	These findings suggested that early CP intervention could enhance the metabolic function of gut microbiota by increasing the release of SCFAs and altering the composition of gut microbiota.
33641986	3	76	from	feces	599:603	arg1	matter					641:646	a dose-dependent matter	624:646	a dose-dependent matter	624:646	The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent matter.
35027132	0	0	theme	mutant	81:86	arg1	sanxanigenens					108:120	a mutant strain Sphingomonas sanxanigenens NX03	79:125	a mutant strain Sphingomonas sanxanigenens NX03	79:125	Characterization of a polysaccharide hydrogel with high elasticity produced by a mutant strain Sphingomonas sanxanigenens NX03.
35027132	4	1	dep	springiness	586:596	arg1	the					582:584	the	582:584	the	582:584	Textural analysis showed the springiness and cohesiveness of Hesan gel was much higher than Sanxan gel, and rheological behaviors indicated it possessed a lower loss factor, and its conformational transition temperatures at different concentrations were obviously lower than Sanxan gel and high-acyl gellan gel, which suggested that Hesan gel was highly elastic and temperature-sensitive.
35027132	0	2	with	hydrogel	37:44	arg1	elasticity					56:65	high elasticity	51:65	high elasticity	51:65	Characterization of a polysaccharide hydrogel with high elasticity produced by a mutant strain Sphingomonas sanxanigenens NX03.
35027132	5	3	theme	Hesan	960:964	arg1	gel					966:968	Hesan gel	960:968	Hesan gel	960:968	Additionally, Hesan gel could be efficiently produced through micro-aerobic static culture in shallow (10.46 ± 0.30 g/L) and deep liquids (3.21 ± 0.32 g/L), which was significantly different from the fermentation of other water-soluble polysaccharides.
35027132	6	4	theme	new	1236:1238	arg1	strain					1247:1252	a new mutant strain	1234:1252	a new mutant strain	1234:1252	In short, this study characterizes a new mutant strain and its polysaccharide products.
35027132	0	5	theme	Sphingomonas	95:106	arg1	sanxanigenens					108:120	a mutant strain Sphingomonas sanxanigenens NX03	79:125	a mutant strain Sphingomonas sanxanigenens NX03	79:125	Characterization of a polysaccharide hydrogel with high elasticity produced by a mutant strain Sphingomonas sanxanigenens NX03.
35027132	4	6	theme	Textural	557:564	arg1	analysis					566:573	Textural analysis	557:573	Textural analysis	557:573	Textural analysis showed the springiness and cohesiveness of Hesan gel was much higher than Sanxan gel, and rheological behaviors indicated it possessed a lower loss factor, and its conformational transition temperatures at different concentrations were obviously lower than Sanxan gel and high-acyl gellan gel, which suggested that Hesan gel was highly elastic and temperature-sensitive.
35027132	2	7	theme	plasma	301:306	arg1	NX03					352:355	a plasma mutagenic strain Sphingomonas sanxanigenens NX03	299:355	a plasma mutagenic strain Sphingomonas sanxanigenens NX03	299:355	Hesan (Highly elastic Sanxan), an exopolysaccharide produced by a plasma mutagenic strain Sphingomonas sanxanigenens NX03, was characterized.
35027132	0	8	theme	strain	88:93	arg1	sanxanigenens					108:120	a mutant strain Sphingomonas sanxanigenens NX03	79:125	a mutant strain Sphingomonas sanxanigenens NX03	79:125	Characterization of a polysaccharide hydrogel with high elasticity produced by a mutant strain Sphingomonas sanxanigenens NX03.
35027132	5	9	theme	other	1162:1166	arg1	polysaccharides					1182:1196	other water-soluble polysaccharides	1162:1196	other water-soluble polysaccharides	1162:1196	Additionally, Hesan gel could be efficiently produced through micro-aerobic static culture in shallow (10.46 ± 0.30 g/L) and deep liquids (3.21 ± 0.32 g/L), which was significantly different from the fermentation of other water-soluble polysaccharides.
35027132	4	10	dep	showed	575:580	arg1	higher					637:642	higher	637:642	higher	637:642	Textural analysis showed the springiness and cohesiveness of Hesan gel was much higher than Sanxan gel, and rheological behaviors indicated it possessed a lower loss factor, and its conformational transition temperatures at different concentrations were obviously lower than Sanxan gel and high-acyl gellan gel, which suggested that Hesan gel was highly elastic and temperature-sensitive.
35027132	5	11	theme	water-soluble	1168:1180	arg1	polysaccharides					1182:1196	other water-soluble polysaccharides	1162:1196	other water-soluble polysaccharides	1162:1196	Additionally, Hesan gel could be efficiently produced through micro-aerobic static culture in shallow (10.46 ± 0.30 g/L) and deep liquids (3.21 ± 0.32 g/L), which was significantly different from the fermentation of other water-soluble polysaccharides.
35027132	4	12	theme	loss	718:721	arg1	factor					723:728	a lower loss factor	710:728	a lower loss factor	710:728	Textural analysis showed the springiness and cohesiveness of Hesan gel was much higher than Sanxan gel, and rheological behaviors indicated it possessed a lower loss factor, and its conformational transition temperatures at different concentrations were obviously lower than Sanxan gel and high-acyl gellan gel, which suggested that Hesan gel was highly elastic and temperature-sensitive.
35027132	3	13	theme	wild-type	478:486	arg1	strain					488:493	wild-type strain NX02	478:498	wild-type strain NX02	478:498	It possessed the same monosaccharide composition as the original polysaccharide Sanxan produced from wild-type strain NX02, but significantly reduced acetyl and glyceryl contents.
35027132	3	14	theme	original	433:440	arg1	composition					414:424	the same monosaccharide composition	390:424	the same monosaccharide composition	390:424	It possessed the same monosaccharide composition as the original polysaccharide Sanxan produced from wild-type strain NX02, but significantly reduced acetyl and glyceryl contents.
35027132	3	14	theme	original	433:440	arg1	Sanxan					457:462	the original polysaccharide Sanxan	429:462	the original polysaccharide Sanxan produced from wild-type strain NX02	429:498	It possessed the same monosaccharide composition as the original polysaccharide Sanxan produced from wild-type strain NX02, but significantly reduced acetyl and glyceryl contents.
35027132	5	15	theme	polysaccharides	1182:1196	arg1	fermentation					1146:1157	the fermentation	1142:1157	the fermentation of other water-soluble polysaccharides	1142:1196	Additionally, Hesan gel could be efficiently produced through micro-aerobic static culture in shallow (10.46 ± 0.30 g/L) and deep liquids (3.21 ± 0.32 g/L), which was significantly different from the fermentation of other water-soluble polysaccharides.
35027132	4	16	theme	Hesan	890:894	arg1	gel					896:898	Hesan gel	890:898	Hesan gel	890:898	Textural analysis showed the springiness and cohesiveness of Hesan gel was much higher than Sanxan gel, and rheological behaviors indicated it possessed a lower loss factor, and its conformational transition temperatures at different concentrations were obviously lower than Sanxan gel and high-acyl gellan gel, which suggested that Hesan gel was highly elastic and temperature-sensitive.
35027132	4	17	theme	Sanxan	832:837	arg1	gel					839:841	Sanxan gel and high-acyl gellan gel	832:866	gel	839:841	Textural analysis showed the springiness and cohesiveness of Hesan gel was much higher than Sanxan gel, and rheological behaviors indicated it possessed a lower loss factor, and its conformational transition temperatures at different concentrations were obviously lower than Sanxan gel and high-acyl gellan gel, which suggested that Hesan gel was highly elastic and temperature-sensitive.
35027132	3	18	theme	same	394:397	arg1	composition					414:424	the same monosaccharide composition	390:424	the same monosaccharide composition	390:424	It possessed the same monosaccharide composition as the original polysaccharide Sanxan produced from wild-type strain NX02, but significantly reduced acetyl and glyceryl contents.
35027132	3	18	theme	same	394:397	arg1	Sanxan					457:462	the original polysaccharide Sanxan	429:462	the original polysaccharide Sanxan produced from wild-type strain NX02	429:498	It possessed the same monosaccharide composition as the original polysaccharide Sanxan produced from wild-type strain NX02, but significantly reduced acetyl and glyceryl contents.
35027132	2	19	theme	sanxanigenens	338:350	arg1	NX03					352:355	a plasma mutagenic strain Sphingomonas sanxanigenens NX03	299:355	a plasma mutagenic strain Sphingomonas sanxanigenens NX03	299:355	Hesan (Highly elastic Sanxan), an exopolysaccharide produced by a plasma mutagenic strain Sphingomonas sanxanigenens NX03, was characterized.
35027132	1	20	theme	attention	202:210	arg1	attention					202:210	attention	202:210	attention	202:210	Microbial polysaccharides as renewable bioproducts have attracted lots of attention in various industries.
35027132	1	20	theme	attention	202:210	arg1	lots					194:197	lots	194:197	lots of attention in various industries	194:232	Microbial polysaccharides as renewable bioproducts have attracted lots of attention in various industries.
35027132	4	21	from	concentrations	791:804	arg1	temperatures					765:776	its conformational transition temperatures	735:776	its conformational transition temperatures at different concentrations	735:804	Textural analysis showed the springiness and cohesiveness of Hesan gel was much higher than Sanxan gel, and rheological behaviors indicated it possessed a lower loss factor, and its conformational transition temperatures at different concentrations were obviously lower than Sanxan gel and high-acyl gellan gel, which suggested that Hesan gel was highly elastic and temperature-sensitive.
35027132	2	22	theme	Sphingomonas	325:336	arg1	NX03					352:355	a plasma mutagenic strain Sphingomonas sanxanigenens NX03	299:355	a plasma mutagenic strain Sphingomonas sanxanigenens NX03	299:355	Hesan (Highly elastic Sanxan), an exopolysaccharide produced by a plasma mutagenic strain Sphingomonas sanxanigenens NX03, was characterized.
35027132	4	23	theme	lower	712:716	arg1	factor					723:728	a lower loss factor	710:728	a lower loss factor	710:728	Textural analysis showed the springiness and cohesiveness of Hesan gel was much higher than Sanxan gel, and rheological behaviors indicated it possessed a lower loss factor, and its conformational transition temperatures at different concentrations were obviously lower than Sanxan gel and high-acyl gellan gel, which suggested that Hesan gel was highly elastic and temperature-sensitive.
35027132	0	24	theme	polysaccharide	22:35	arg1	hydrogel					37:44	a polysaccharide hydrogel	20:44	a polysaccharide hydrogel with high elasticity	20:65	Characterization of a polysaccharide hydrogel with high elasticity produced by a mutant strain Sphingomonas sanxanigenens NX03.
35027132	4	25	theme	different	781:789	arg1	concentrations					791:804	different concentrations	781:804	different concentrations	781:804	Textural analysis showed the springiness and cohesiveness of Hesan gel was much higher than Sanxan gel, and rheological behaviors indicated it possessed a lower loss factor, and its conformational transition temperatures at different concentrations were obviously lower than Sanxan gel and high-acyl gellan gel, which suggested that Hesan gel was highly elastic and temperature-sensitive.
35027132	2	26	theme	strain	318:323	arg1	NX03					352:355	a plasma mutagenic strain Sphingomonas sanxanigenens NX03	299:355	a plasma mutagenic strain Sphingomonas sanxanigenens NX03	299:355	Hesan (Highly elastic Sanxan), an exopolysaccharide produced by a plasma mutagenic strain Sphingomonas sanxanigenens NX03, was characterized.
35027132	1	27	theme	various	215:221	arg1	industries					223:232	various industries	215:232	various industries	215:232	Microbial polysaccharides as renewable bioproducts have attracted lots of attention in various industries.
35027132	3	28	theme	monosaccharide	399:412	arg1	composition					414:424	the same monosaccharide composition	390:424	the same monosaccharide composition	390:424	It possessed the same monosaccharide composition as the original polysaccharide Sanxan produced from wild-type strain NX02, but significantly reduced acetyl and glyceryl contents.
35027132	3	28	theme	monosaccharide	399:412	arg1	Sanxan					457:462	the original polysaccharide Sanxan	429:462	the original polysaccharide Sanxan produced from wild-type strain NX02	429:498	It possessed the same monosaccharide composition as the original polysaccharide Sanxan produced from wild-type strain NX02, but significantly reduced acetyl and glyceryl contents.
35027132	4	29	theme	gel	624:626	arg1	springiness					586:596	springiness	586:596	springiness	586:596	Textural analysis showed the springiness and cohesiveness of Hesan gel was much higher than Sanxan gel, and rheological behaviors indicated it possessed a lower loss factor, and its conformational transition temperatures at different concentrations were obviously lower than Sanxan gel and high-acyl gellan gel, which suggested that Hesan gel was highly elastic and temperature-sensitive.
35027132	4	29	theme	gel	624:626	arg1	cohesiveness					602:613	cohesiveness	602:613	cohesiveness	602:613	Textural analysis showed the springiness and cohesiveness of Hesan gel was much higher than Sanxan gel, and rheological behaviors indicated it possessed a lower loss factor, and its conformational transition temperatures at different concentrations were obviously lower than Sanxan gel and high-acyl gellan gel, which suggested that Hesan gel was highly elastic and temperature-sensitive.
35027132	5	30	theme	micro-aerobic	1008:1020	arg1	culture					1029:1035	micro-aerobic static culture	1008:1035	micro-aerobic static culture in shallow (10.46 ± 0.30 g/L) and deep liquids (3.21 ± 0.32 g/L), which was significantly different from the fermentation of other water-soluble polysaccharides	1008:1196	Additionally, Hesan gel could be efficiently produced through micro-aerobic static culture in shallow (10.46 ± 0.30 g/L) and deep liquids (3.21 ± 0.32 g/L), which was significantly different from the fermentation of other water-soluble polysaccharides.
35027132	2	31	theme	mutagenic	308:316	arg1	NX03					352:355	a plasma mutagenic strain Sphingomonas sanxanigenens NX03	299:355	a plasma mutagenic strain Sphingomonas sanxanigenens NX03	299:355	Hesan (Highly elastic Sanxan), an exopolysaccharide produced by a plasma mutagenic strain Sphingomonas sanxanigenens NX03, was characterized.
35027132	4	32	theme	rheological	665:675	arg1	behaviors					677:685	rheological behaviors	665:685	rheological behaviors indicated it	665:698	Textural analysis showed the springiness and cohesiveness of Hesan gel was much higher than Sanxan gel, and rheological behaviors indicated it possessed a lower loss factor, and its conformational transition temperatures at different concentrations were obviously lower than Sanxan gel and high-acyl gellan gel, which suggested that Hesan gel was highly elastic and temperature-sensitive.
35027132	3	33	theme	polysaccharide	442:455	arg1	composition					414:424	the same monosaccharide composition	390:424	the same monosaccharide composition	390:424	It possessed the same monosaccharide composition as the original polysaccharide Sanxan produced from wild-type strain NX02, but significantly reduced acetyl and glyceryl contents.
35027132	3	33	theme	polysaccharide	442:455	arg1	Sanxan					457:462	the original polysaccharide Sanxan	429:462	the original polysaccharide Sanxan produced from wild-type strain NX02	429:498	It possessed the same monosaccharide composition as the original polysaccharide Sanxan produced from wild-type strain NX02, but significantly reduced acetyl and glyceryl contents.
35027132	3	34	theme	glyceryl	538:545	arg1	contents					547:554	glyceryl contents	538:554	glyceryl contents	538:554	It possessed the same monosaccharide composition as the original polysaccharide Sanxan produced from wild-type strain NX02, but significantly reduced acetyl and glyceryl contents.
35027132	0	35	theme	hydrogel	37:44	arg1	Characterization					0:15	Characterization	0:15	Characterization of a polysaccharide hydrogel with high elasticity	0:65	Characterization of a polysaccharide hydrogel with high elasticity produced by a mutant strain Sphingomonas sanxanigenens NX03.
35027132	2	36	theme	elastic	249:255	arg1	Sanxan					257:262	Highly elastic Sanxan	242:262	Highly elastic Sanxan	242:262	Hesan (Highly elastic Sanxan), an exopolysaccharide produced by a plasma mutagenic strain Sphingomonas sanxanigenens NX03, was characterized.
35027132	2	36	theme	elastic	249:255	arg1	Hesan					235:239	Hesan	235:239	Hesan (Highly elastic Sanxan)	235:263	Hesan (Highly elastic Sanxan), an exopolysaccharide produced by a plasma mutagenic strain Sphingomonas sanxanigenens NX03, was characterized.
35027132	4	37	theme	Hesan	618:622	arg1	gel					624:626	Hesan gel	618:626	Hesan gel	618:626	Textural analysis showed the springiness and cohesiveness of Hesan gel was much higher than Sanxan gel, and rheological behaviors indicated it possessed a lower loss factor, and its conformational transition temperatures at different concentrations were obviously lower than Sanxan gel and high-acyl gellan gel, which suggested that Hesan gel was highly elastic and temperature-sensitive.
35027132	5	38	from	fermentation	1146:1157	arg1	different					1127:1135	different	1127:1135	different	1127:1135	Additionally, Hesan gel could be efficiently produced through micro-aerobic static culture in shallow (10.46 ± 0.30 g/L) and deep liquids (3.21 ± 0.32 g/L), which was significantly different from the fermentation of other water-soluble polysaccharides.
35027132	4	39	theme	transition	754:763	arg1	temperatures					765:776	its conformational transition temperatures	735:776	its conformational transition temperatures at different concentrations	735:804	Textural analysis showed the springiness and cohesiveness of Hesan gel was much higher than Sanxan gel, and rheological behaviors indicated it possessed a lower loss factor, and its conformational transition temperatures at different concentrations were obviously lower than Sanxan gel and high-acyl gellan gel, which suggested that Hesan gel was highly elastic and temperature-sensitive.
35027132	0	40	theme	high	51:54	arg1	elasticity					56:65	high elasticity	51:65	high elasticity	51:65	Characterization of a polysaccharide hydrogel with high elasticity produced by a mutant strain Sphingomonas sanxanigenens NX03.
35027132	1	41	theme	Microbial	128:136	arg1	polysaccharides					138:152	Microbial polysaccharides	128:152	Microbial polysaccharides as renewable bioproducts	128:177	Microbial polysaccharides as renewable bioproducts have attracted lots of attention in various industries.
35027132	6	42	theme	polysaccharide	1262:1275	arg1	products					1277:1284	its polysaccharide products	1258:1284	its polysaccharide products	1258:1284	In short, this study characterizes a new mutant strain and its polysaccharide products.
35027132	4	43	theme	high-acyl	847:855	arg1	gel					864:866	Sanxan gel and high-acyl gellan gel	832:866	gel	864:866	Textural analysis showed the springiness and cohesiveness of Hesan gel was much higher than Sanxan gel, and rheological behaviors indicated it possessed a lower loss factor, and its conformational transition temperatures at different concentrations were obviously lower than Sanxan gel and high-acyl gellan gel, which suggested that Hesan gel was highly elastic and temperature-sensitive.
35027132	3	44	contain	possessed	380:388	arg2	Sanxan					457:462	the original polysaccharide Sanxan	429:462	the original polysaccharide Sanxan produced from wild-type strain NX02	429:498	It possessed the same monosaccharide composition as the original polysaccharide Sanxan produced from wild-type strain NX02, but significantly reduced acetyl and glyceryl contents.
35027132	3	44	contain	possessed	380:388	arg1	It					377:378	It	377:378	It	377:378	It possessed the same monosaccharide composition as the original polysaccharide Sanxan produced from wild-type strain NX02, but significantly reduced acetyl and glyceryl contents.
35027132	3	44	contain	possessed	380:388	arg2	composition					414:424	the same monosaccharide composition	390:424	the same monosaccharide composition	390:424	It possessed the same monosaccharide composition as the original polysaccharide Sanxan produced from wild-type strain NX02, but significantly reduced acetyl and glyceryl contents.
35027132	5	45	theme	static	1022:1027	arg1	culture					1029:1035	micro-aerobic static culture	1008:1035	micro-aerobic static culture in shallow (10.46 ± 0.30 g/L) and deep liquids (3.21 ± 0.32 g/L), which was significantly different from the fermentation of other water-soluble polysaccharides	1008:1196	Additionally, Hesan gel could be efficiently produced through micro-aerobic static culture in shallow (10.46 ± 0.30 g/L) and deep liquids (3.21 ± 0.32 g/L), which was significantly different from the fermentation of other water-soluble polysaccharides.
35027132	6	46	theme	mutant	1240:1245	arg1	strain					1247:1252	a new mutant strain	1234:1252	a new mutant strain	1234:1252	In short, this study characterizes a new mutant strain and its polysaccharide products.
35027132	4	47	contain	possessed	700:708	arg2	factor					723:728	a lower loss factor	710:728	a lower loss factor	710:728	Textural analysis showed the springiness and cohesiveness of Hesan gel was much higher than Sanxan gel, and rheological behaviors indicated it possessed a lower loss factor, and its conformational transition temperatures at different concentrations were obviously lower than Sanxan gel and high-acyl gellan gel, which suggested that Hesan gel was highly elastic and temperature-sensitive.
35027132	4	47	contain	possessed	700:708	arg1	behaviors					677:685	rheological behaviors	665:685	rheological behaviors indicated it	665:698	Textural analysis showed the springiness and cohesiveness of Hesan gel was much higher than Sanxan gel, and rheological behaviors indicated it possessed a lower loss factor, and its conformational transition temperatures at different concentrations were obviously lower than Sanxan gel and high-acyl gellan gel, which suggested that Hesan gel was highly elastic and temperature-sensitive.
35027132	5	48	dep	shallow	1040:1046	arg1	liquids					1076:1082	liquids	1076:1082	liquids	1076:1082	Additionally, Hesan gel could be efficiently produced through micro-aerobic static culture in shallow (10.46 ± 0.30 g/L) and deep liquids (3.21 ± 0.32 g/L), which was significantly different from the fermentation of other water-soluble polysaccharides.
35027132	4	49	theme	Sanxan	649:654	arg1	gel					656:658	Sanxan gel	649:658	Sanxan gel	649:658	Textural analysis showed the springiness and cohesiveness of Hesan gel was much higher than Sanxan gel, and rheological behaviors indicated it possessed a lower loss factor, and its conformational transition temperatures at different concentrations were obviously lower than Sanxan gel and high-acyl gellan gel, which suggested that Hesan gel was highly elastic and temperature-sensitive.
35027132	1	50	theme	renewable	157:165	arg1	bioproducts					167:177	renewable bioproducts	157:177	renewable bioproducts	157:177	Microbial polysaccharides as renewable bioproducts have attracted lots of attention in various industries.
35027132	5	51	from	culture	1029:1035	arg1	deep					1071:1074	deep	1071:1074	deep	1071:1074	Additionally, Hesan gel could be efficiently produced through micro-aerobic static culture in shallow (10.46 ± 0.30 g/L) and deep liquids (3.21 ± 0.32 g/L), which was significantly different from the fermentation of other water-soluble polysaccharides.
35027132	5	51	from	culture	1029:1035	arg1	shallow					1040:1046	shallow	1040:1046	shallow	1040:1046	Additionally, Hesan gel could be efficiently produced through micro-aerobic static culture in shallow (10.46 ± 0.30 g/L) and deep liquids (3.21 ± 0.32 g/L), which was significantly different from the fermentation of other water-soluble polysaccharides.
35027132	5	51	from	culture	1029:1035	arg1	3.21 ± 0.32 g/L					1085:1099	3.21 ± 0.32 g/L	1085:1099	3.21 ± 0.32 g/L	1085:1099	Additionally, Hesan gel could be efficiently produced through micro-aerobic static culture in shallow (10.46 ± 0.30 g/L) and deep liquids (3.21 ± 0.32 g/L), which was significantly different from the fermentation of other water-soluble polysaccharides.
35027132	5	51	from	culture	1029:1035	arg1	10.46 ± 0.30 g/L					1049:1064	10.46 ± 0.30 g/L	1049:1064	10.46 ± 0.30 g/L	1049:1064	Additionally, Hesan gel could be efficiently produced through micro-aerobic static culture in shallow (10.46 ± 0.30 g/L) and deep liquids (3.21 ± 0.32 g/L), which was significantly different from the fermentation of other water-soluble polysaccharides.
35027132	4	52	theme	gellan	857:862	arg1	gel					864:866	Sanxan gel and high-acyl gellan gel	832:866	gel	864:866	Textural analysis showed the springiness and cohesiveness of Hesan gel was much higher than Sanxan gel, and rheological behaviors indicated it possessed a lower loss factor, and its conformational transition temperatures at different concentrations were obviously lower than Sanxan gel and high-acyl gellan gel, which suggested that Hesan gel was highly elastic and temperature-sensitive.
35027132	1	53	from	lots	194:197	arg1	industries					223:232	various industries	215:232	various industries	215:232	Microbial polysaccharides as renewable bioproducts have attracted lots of attention in various industries.
35027132	4	54	theme	conformational	739:752	arg1	temperatures					765:776	its conformational transition temperatures	735:776	its conformational transition temperatures at different concentrations	735:804	Textural analysis showed the springiness and cohesiveness of Hesan gel was much higher than Sanxan gel, and rheological behaviors indicated it possessed a lower loss factor, and its conformational transition temperatures at different concentrations were obviously lower than Sanxan gel and high-acyl gellan gel, which suggested that Hesan gel was highly elastic and temperature-sensitive.
32928385	2	0	theme	global	466:471	arg1	markets					473:479	global markets	466:479	global markets	466:479	Since the expiration of the patent of the innovator epoetin alfa, numerous rhEPO products have emerged in global markets.
32928385	1	1	theme	chronic	320:326	arg1	disease					335:341	chronic kidney disease	320:341	chronic kidney disease	320:341	Recombinant human erythropoietin (rhEPO) is a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy.
32928385	4	2	theme	analytical	873:882	arg1	methodology					884:894	an effective analytical methodology	860:894	an effective analytical methodology to monitor the product quality attributes of rhEPO analogues	860:955	The focus of this study is placed on the overall glycosylation profiling, O-glycan profiling, and N-glycan mapping by UPLC-MS with an aim to develop an effective analytical methodology to monitor the product quality attributes of rhEPO analogues.
32928385	1	3	theme	Recombinant	156:166	arg1	rhEPO					190:194	rhEPO	190:194	rhEPO	190:194	Recombinant human erythropoietin (rhEPO) is a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy.
32928385	1	3	theme	Recombinant	156:166	arg1	erythropoietin					174:187	Recombinant human erythropoietin	156:187	Recombinant human erythropoietin (rhEPO)	156:195	Recombinant human erythropoietin (rhEPO) is a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy.
32928385	1	3	theme	Recombinant	156:166	arg1	glycoprotein					202:213	a glycoprotein	200:213	a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy	200:357	Recombinant human erythropoietin (rhEPO) is a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy.
32928385	4	4	with	UPLC-MS	829:835	arg1	aim					845:847	an aim to develop an effective analytical methodology to monitor the product quality attributes of rhEPO analogues	842:955	an aim to develop an effective analytical methodology to monitor the product quality attributes of rhEPO analogues	842:955	The focus of this study is placed on the overall glycosylation profiling, O-glycan profiling, and N-glycan mapping by UPLC-MS with an aim to develop an effective analytical methodology to monitor the product quality attributes of rhEPO analogues.
32928385	3	5	theme	Chinese	695:701	arg1	market					703:708	the Chinese market	691:708	the Chinese market	691:708	As described here, multiple complementary analytical approaches are utilized for the extensive characterization of rhEPO molecules, and more importantly for the structural comparison of the rhEPO analogues on the Chinese market.
32928385	0	6	from	assessment	8:17	arg1	similarity					37:46	the structural similarity	22:46	the structural similarity of recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring	22:153	UPLC-MS assessment on the structural similarity of recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring.
32928385	6	7	from	profile	1122:1128	arg1	acids					1273:1277	sialic acids	1266:1277	sialic acids	1266:1277	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	1	8	theme	human	168:172	arg1	rhEPO					190:194	rhEPO	190:194	rhEPO	190:194	Recombinant human erythropoietin (rhEPO) is a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy.
32928385	1	8	theme	human	168:172	arg1	erythropoietin					174:187	Recombinant human erythropoietin	156:187	Recombinant human erythropoietin (rhEPO)	156:195	Recombinant human erythropoietin (rhEPO) is a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy.
32928385	1	8	theme	human	168:172	arg1	glycoprotein					202:213	a glycoprotein	200:213	a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy	200:357	Recombinant human erythropoietin (rhEPO) is a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy.
32928385	5	9	theme	rhEPO	962:966	arg1	analogues					968:976	Two rhEPO analogues	958:976	Two rhEPO analogues manufactured in China	958:998	Two rhEPO analogues manufactured in China were analyzed to demonstrate the principle of the developed methods.
32928385	0	10	theme	analogues	92:100	arg1	similarity					37:46	the structural similarity	22:46	the structural similarity of recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring	22:153	UPLC-MS assessment on the structural similarity of recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring.
32928385	4	11	theme	N-glycan	809:816	arg1	mapping					818:824	N-glycan mapping	809:824	N-glycan mapping	809:824	The focus of this study is placed on the overall glycosylation profiling, O-glycan profiling, and N-glycan mapping by UPLC-MS with an aim to develop an effective analytical methodology to monitor the product quality attributes of rhEPO analogues.
32928385	3	12	theme	analytical	524:533	arg1	approaches					535:544	multiple complementary analytical approaches	501:544	multiple complementary analytical approaches	501:544	As described here, multiple complementary analytical approaches are utilized for the extensive characterization of rhEPO molecules, and more importantly for the structural comparison of the rhEPO analogues on the Chinese market.
32928385	4	13	theme	quality	919:925	arg1	attributes					927:936	the product quality attributes	907:936	the product quality attributes of rhEPO analogues	907:955	The focus of this study is placed on the overall glycosylation profiling, O-glycan profiling, and N-glycan mapping by UPLC-MS with an aim to develop an effective analytical methodology to monitor the product quality attributes of rhEPO analogues.
32928385	3	14	theme	analogues	678:686	arg1	comparison					654:663	the structural comparison	639:663	the structural comparison of the rhEPO analogues on the Chinese market	639:708	As described here, multiple complementary analytical approaches are utilized for the extensive characterization of rhEPO molecules, and more importantly for the structural comparison of the rhEPO analogues on the Chinese market.
32928385	6	15	theme	acids	1173:1177	arg1	distribution					1150:1161	the distribution	1146:1161	the distribution of sialic acids across multi-antennary structures	1146:1211	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	4	16	theme	rhEPO	941:945	arg1	analogues					947:955	rhEPO analogues	941:955	rhEPO analogues	941:955	The focus of this study is placed on the overall glycosylation profiling, O-glycan profiling, and N-glycan mapping by UPLC-MS with an aim to develop an effective analytical methodology to monitor the product quality attributes of rhEPO analogues.
32928385	7	17	theme	analytical	1403:1412	arg1	tool					1414:1417	an effective analytical tool	1390:1417	an effective analytical tool to characterize and monitor the glycosylation profiles among rhEPO analogues in order to detect and account for the divergence between rhEPO products, as well as the presence of unusual or unexpected glycans	1390:1625	The study demonstrates that UPLC-MS is an effective analytical tool to characterize and monitor the glycosylation profiles among rhEPO analogues in order to detect and account for the divergence between rhEPO products, as well as the presence of unusual or unexpected glycans.
32928385	7	17	theme	analytical	1403:1412	arg1	UPLC-MS					1379:1385	UPLC-MS	1379:1385	UPLC-MS	1379:1385	The study demonstrates that UPLC-MS is an effective analytical tool to characterize and monitor the glycosylation profiles among rhEPO analogues in order to detect and account for the divergence between rhEPO products, as well as the presence of unusual or unexpected glycans.
32928385	3	18	theme	multiple	501:508	arg1	approaches					535:544	multiple complementary analytical approaches	501:544	multiple complementary analytical approaches	501:544	As described here, multiple complementary analytical approaches are utilized for the extensive characterization of rhEPO molecules, and more importantly for the structural comparison of the rhEPO analogues on the Chinese market.
32928385	7	19	theme	glycans	1619:1625	arg1	presence					1585:1592	the presence	1581:1592	the presence of unusual or unexpected glycans	1581:1625	The study demonstrates that UPLC-MS is an effective analytical tool to characterize and monitor the glycosylation profiles among rhEPO analogues in order to detect and account for the divergence between rhEPO products, as well as the presence of unusual or unexpected glycans.
32928385	0	20	from	similarity	37:46	arg1	manufacturers					107:119	manufacturers	107:119	manufacturers in China for attribute monitoring	107:153	UPLC-MS assessment on the structural similarity of recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring.
32928385	4	21	theme	O-glycan	785:792	arg1	profiling					794:802	O-glycan profiling	785:802	O-glycan profiling	785:802	The focus of this study is placed on the overall glycosylation profiling, O-glycan profiling, and N-glycan mapping by UPLC-MS with an aim to develop an effective analytical methodology to monitor the product quality attributes of rhEPO analogues.
32928385	0	22	theme	UPLC-MS	0:6	arg1	assessment					8:17	UPLC-MS assessment	0:17	UPLC-MS assessment on the structural similarity of recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring.	0:154	UPLC-MS assessment on the structural similarity of recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring.
32928385	4	23	theme	product	911:917	arg1	attributes					927:936	the product quality attributes	907:936	the product quality attributes of rhEPO analogues	907:955	The focus of this study is placed on the overall glycosylation profiling, O-glycan profiling, and N-glycan mapping by UPLC-MS with an aim to develop an effective analytical methodology to monitor the product quality attributes of rhEPO analogues.
32928385	2	24	theme	numerous	426:433	arg1	products					441:448	numerous rhEPO products	426:448	numerous rhEPO products	426:448	Since the expiration of the patent of the innovator epoetin alfa, numerous rhEPO products have emerged in global markets.
32928385	7	25	theme	rhEPO	1480:1484	arg1	analogues					1486:1494	rhEPO analogues	1480:1494	rhEPO analogues in order to detect and account for the divergence between rhEPO products, as well as the presence of unusual or unexpected glycans	1480:1625	The study demonstrates that UPLC-MS is an effective analytical tool to characterize and monitor the glycosylation profiles among rhEPO analogues in order to detect and account for the divergence between rhEPO products, as well as the presence of unusual or unexpected glycans.
32928385	6	26	from	O-acetylation	1249:1261	arg1	acids					1273:1277	sialic acids	1266:1277	sialic acids	1266:1277	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	3	27	from	comparison	654:663	arg1	market					703:708	the Chinese market	691:708	the Chinese market	691:708	As described here, multiple complementary analytical approaches are utilized for the extensive characterization of rhEPO molecules, and more importantly for the structural comparison of the rhEPO analogues on the Chinese market.
32928385	6	28	theme	rhEPO	1074:1078	arg1	product					1080:1086	Each rhEPO product	1069:1086	Each rhEPO product	1069:1086	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	1	29	theme	kidney	328:333	arg1	disease					335:341	chronic kidney disease	320:341	chronic kidney disease	320:341	Recombinant human erythropoietin (rhEPO) is a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy.
32928385	1	30	theme	red	268:270	arg1	production					283:292	red blood cell production	268:292	red blood cell production	268:292	Recombinant human erythropoietin (rhEPO) is a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy.
32928385	1	31	gly	glycoprotein	202:213	arg1	erythropoietin					174:187	Recombinant human erythropoietin	156:187	Recombinant human erythropoietin (rhEPO)	156:195	Recombinant human erythropoietin (rhEPO) is a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy.
32928385	1	31	gly	glycoprotein	202:213	arg1	glycoprotein					202:213	a glycoprotein	200:213	a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy	200:357	Recombinant human erythropoietin (rhEPO) is a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy.
32928385	1	31	gly	glycoprotein	202:213	arg1	hormone					237:243	the main hormone	228:243	the main hormone involved in regulating red blood cell production	228:292	Recombinant human erythropoietin (rhEPO) is a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy.
32928385	5	32	theme	methods	1060:1066	arg1	principle					1033:1041	the principle	1029:1041	the principle of the developed methods	1029:1066	Two rhEPO analogues manufactured in China were analyzed to demonstrate the principle of the developed methods.
32928385	2	33	theme	alfa	420:423	arg1	patent					388:393	the patent	384:393	the patent of the innovator epoetin alfa	384:423	Since the expiration of the patent of the innovator epoetin alfa, numerous rhEPO products have emerged in global markets.
32928385	1	34	theme	blood	272:276	arg1	production					283:292	red blood cell production	268:292	red blood cell production	268:292	Recombinant human erythropoietin (rhEPO) is a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy.
32928385	2	35	theme	innovator	402:410	arg1	alfa					420:423	the innovator epoetin alfa	398:423	the innovator epoetin alfa	398:423	Since the expiration of the patent of the innovator epoetin alfa, numerous rhEPO products have emerged in global markets.
32928385	2	36	theme	epoetin	412:418	arg1	alfa					420:423	the innovator epoetin alfa	398:423	the innovator epoetin alfa	398:423	Since the expiration of the patent of the innovator epoetin alfa, numerous rhEPO products have emerged in global markets.
32928385	1	37	theme	cell	278:281	arg1	production					283:292	red blood cell production	268:292	red blood cell production	268:292	Recombinant human erythropoietin (rhEPO) is a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy.
32928385	4	38	theme	overall	752:758	arg1	profiling					774:782	the overall glycosylation profiling	748:782	the overall glycosylation profiling	748:782	The focus of this study is placed on the overall glycosylation profiling, O-glycan profiling, and N-glycan mapping by UPLC-MS with an aim to develop an effective analytical methodology to monitor the product quality attributes of rhEPO analogues.
32928385	5	39	theme	developed	1050:1058	arg1	methods					1060:1066	the developed methods	1046:1066	the developed methods	1046:1066	Two rhEPO analogues manufactured in China were analyzed to demonstrate the principle of the developed methods.
32928385	4	40	theme	study	729:733	arg1	focus					715:719	The focus	711:719	The focus of this study	711:733	The focus of this study is placed on the overall glycosylation profiling, O-glycan profiling, and N-glycan mapping by UPLC-MS with an aim to develop an effective analytical methodology to monitor the product quality attributes of rhEPO analogues.
32928385	3	41	from	analogues	678:686	arg1	market					703:708	the Chinese market	691:708	the Chinese market	691:708	As described here, multiple complementary analytical approaches are utilized for the extensive characterization of rhEPO molecules, and more importantly for the structural comparison of the rhEPO analogues on the Chinese market.
32928385	0	42	theme	attribute	134:142	arg1	monitoring					144:153	attribute monitoring	134:153	attribute monitoring	134:153	UPLC-MS assessment on the structural similarity of recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring.
32928385	3	43	theme	structural	643:652	arg1	comparison					654:663	the structural comparison	639:663	the structural comparison of the rhEPO analogues on the Chinese market	639:708	As described here, multiple complementary analytical approaches are utilized for the extensive characterization of rhEPO molecules, and more importantly for the structural comparison of the rhEPO analogues on the Chinese market.
32928385	7	44	theme	effective	1393:1401	arg1	tool					1414:1417	an effective analytical tool	1390:1417	an effective analytical tool to characterize and monitor the glycosylation profiles among rhEPO analogues in order to detect and account for the divergence between rhEPO products, as well as the presence of unusual or unexpected glycans	1390:1625	The study demonstrates that UPLC-MS is an effective analytical tool to characterize and monitor the glycosylation profiles among rhEPO analogues in order to detect and account for the divergence between rhEPO products, as well as the presence of unusual or unexpected glycans.
32928385	7	44	theme	effective	1393:1401	arg1	UPLC-MS					1379:1385	UPLC-MS	1379:1385	UPLC-MS	1379:1385	The study demonstrates that UPLC-MS is an effective analytical tool to characterize and monitor the glycosylation profiles among rhEPO analogues in order to detect and account for the divergence between rhEPO products, as well as the presence of unusual or unexpected glycans.
32928385	6	45	with	antennae	1310:1317	arg1	units					1344:1348	N-acetyllactosamine units	1324:1348	N-acetyllactosamine units	1324:1348	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	0	46	theme	structural	26:35	arg1	similarity					37:46	the structural similarity	22:46	the structural similarity of recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring	22:153	UPLC-MS assessment on the structural similarity of recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring.
32928385	6	47	theme	N-acetyllactosamine	1324:1342	arg1	units					1344:1348	N-acetyllactosamine units	1324:1348	N-acetyllactosamine units	1324:1348	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	3	48	theme	rhEPO	597:601	arg1	molecules					603:611	rhEPO molecules	597:611	rhEPO molecules	597:611	As described here, multiple complementary analytical approaches are utilized for the extensive characterization of rhEPO molecules, and more importantly for the structural comparison of the rhEPO analogues on the Chinese market.
32928385	2	49	theme	patent	388:393	arg1	expiration					370:379	the expiration	366:379	the expiration of the patent of the innovator epoetin alfa	366:423	Since the expiration of the patent of the innovator epoetin alfa, numerous rhEPO products have emerged in global markets.
32928385	0	50	theme	recombinant	51:61	arg1	rhEPO					85:89	rhEPO	85:89	rhEPO	85:89	UPLC-MS assessment on the structural similarity of recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring.
32928385	0	50	theme	recombinant	51:61	arg1	erythropoietin					69:82	recombinant human erythropoietin	51:82	recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring	51:153	UPLC-MS assessment on the structural similarity of recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring.
32928385	7	51	theme	rhEPO	1554:1558	arg1	products					1560:1567	rhEPO products	1554:1567	rhEPO products	1554:1567	The study demonstrates that UPLC-MS is an effective analytical tool to characterize and monitor the glycosylation profiles among rhEPO analogues in order to detect and account for the divergence between rhEPO products, as well as the presence of unusual or unexpected glycans.
32928385	4	52	theme	effective	863:871	arg1	methodology					884:894	an effective analytical methodology	860:894	an effective analytical methodology to monitor the product quality attributes of rhEPO analogues	860:955	The focus of this study is placed on the overall glycosylation profiling, O-glycan profiling, and N-glycan mapping by UPLC-MS with an aim to develop an effective analytical methodology to monitor the product quality attributes of rhEPO analogues.
32928385	4	53	theme	analogues	947:955	arg1	attributes					927:936	the product quality attributes	907:936	the product quality attributes of rhEPO analogues	907:955	The focus of this study is placed on the overall glycosylation profiling, O-glycan profiling, and N-glycan mapping by UPLC-MS with an aim to develop an effective analytical methodology to monitor the product quality attributes of rhEPO analogues.
32928385	3	54	theme	extensive	567:575	arg1	characterization					577:592	the extensive characterization	563:592	the extensive characterization of rhEPO molecules	563:611	As described here, multiple complementary analytical approaches are utilized for the extensive characterization of rhEPO molecules, and more importantly for the structural comparison of the rhEPO analogues on the Chinese market.
32928385	2	55	theme	rhEPO	435:439	arg1	products					441:448	numerous rhEPO products	426:448	numerous rhEPO products	426:448	Since the expiration of the patent of the innovator epoetin alfa, numerous rhEPO products have emerged in global markets.
32928385	7	56	theme	unexpected	1608:1617	arg1	glycans					1619:1625	unusual or unexpected glycans	1597:1625	unusual or unexpected glycans	1597:1625	The study demonstrates that UPLC-MS is an effective analytical tool to characterize and monitor the glycosylation profiles among rhEPO analogues in order to detect and account for the divergence between rhEPO products, as well as the presence of unusual or unexpected glycans.
32928385	6	57	theme	antennae	1310:1317	arg1	O-acetylation					1249:1261	O-acetylation	1249:1261	O-acetylation on sialic acids	1249:1277	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	6	57	theme	antennae	1310:1317	arg1	profile					1122:1128	a characteristic glycoform profile	1095:1128	a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures	1095:1211	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	6	57	theme	antennae	1310:1317	arg1	occurrence					1218:1227	the occurrence	1214:1227	the occurrence of O-glycosylation	1214:1246	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	6	57	theme	antennae	1310:1317	arg1	extension					1288:1296	the extension	1284:1296	the extension of N-glycan antennae with N-acetyllactosamine units	1284:1348	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	0	58	from	manufacturers	107:119	arg1	similarity					37:46	the structural similarity	22:46	the structural similarity of recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring	22:153	UPLC-MS assessment on the structural similarity of recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring.
32928385	0	58	from	manufacturers	107:119	arg1	China					124:128	China	124:128	China	124:128	UPLC-MS assessment on the structural similarity of recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring.
32928385	0	58	from	manufacturers	107:119	arg1	analogues					92:100	recombinant human erythropoietin (rhEPO) analogues	51:100	recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring	51:153	UPLC-MS assessment on the structural similarity of recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring.
32928385	6	59	theme	sialic	1266:1271	arg1	acids					1273:1277	sialic acids	1266:1277	sialic acids	1266:1277	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	7	60	theme	glycosylation	1451:1463	arg1	profiles					1465:1472	the glycosylation profiles	1447:1472	the glycosylation profiles among rhEPO analogues in order to detect and account for the divergence between rhEPO products, as well as the presence of unusual or unexpected glycans	1447:1625	The study demonstrates that UPLC-MS is an effective analytical tool to characterize and monitor the glycosylation profiles among rhEPO analogues in order to detect and account for the divergence between rhEPO products, as well as the presence of unusual or unexpected glycans.
32928385	6	61	theme	N-glycan	1301:1308	arg1	antennae					1310:1317	N-glycan antennae	1301:1317	N-glycan antennae with N-acetyllactosamine units	1301:1348	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	3	62	theme	rhEPO	672:676	arg1	analogues					678:686	the rhEPO analogues	668:686	the rhEPO analogues on the Chinese market	668:708	As described here, multiple complementary analytical approaches are utilized for the extensive characterization of rhEPO molecules, and more importantly for the structural comparison of the rhEPO analogues on the Chinese market.
32928385	6	63	theme	O-glycosylation	1232:1246	arg1	O-acetylation					1249:1261	O-acetylation	1249:1261	O-acetylation on sialic acids	1249:1277	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	6	63	theme	O-glycosylation	1232:1246	arg1	profile					1122:1128	a characteristic glycoform profile	1095:1128	a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures	1095:1211	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	6	63	theme	O-glycosylation	1232:1246	arg1	occurrence					1218:1227	the occurrence	1214:1227	the occurrence of O-glycosylation	1214:1246	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	6	63	theme	O-glycosylation	1232:1246	arg1	extension					1288:1296	the extension	1284:1296	the extension of N-glycan antennae with N-acetyllactosamine units	1284:1348	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	6	64	theme	glycoform	1112:1120	arg1	profile					1122:1128	a characteristic glycoform profile	1095:1128	a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures	1095:1211	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	6	65	from	occurrence	1218:1227	arg1	acids					1273:1277	sialic acids	1266:1277	sialic acids	1266:1277	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	7	66	theme	unusual	1597:1603	arg1	glycans					1619:1625	unusual or unexpected glycans	1597:1625	unusual or unexpected glycans	1597:1625	The study demonstrates that UPLC-MS is an effective analytical tool to characterize and monitor the glycosylation profiles among rhEPO analogues in order to detect and account for the divergence between rhEPO products, as well as the presence of unusual or unexpected glycans.
32928385	3	67	theme	complementary	510:522	arg1	approaches					535:544	multiple complementary analytical approaches	501:544	multiple complementary analytical approaches	501:544	As described here, multiple complementary analytical approaches are utilized for the extensive characterization of rhEPO molecules, and more importantly for the structural comparison of the rhEPO analogues on the Chinese market.
32928385	4	68	theme	glycosylation	760:772	arg1	profiling					774:782	the overall glycosylation profiling	748:782	the overall glycosylation profiling	748:782	The focus of this study is placed on the overall glycosylation profiling, O-glycan profiling, and N-glycan mapping by UPLC-MS with an aim to develop an effective analytical methodology to monitor the product quality attributes of rhEPO analogues.
32928385	6	69	theme	characteristic	1097:1110	arg1	profile					1122:1128	a characteristic glycoform profile	1095:1128	a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures	1095:1211	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	0	70	theme	erythropoietin	69:82	arg1	analogues					92:100	recombinant human erythropoietin (rhEPO) analogues	51:100	recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring	51:153	UPLC-MS assessment on the structural similarity of recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring.
32928385	3	71	theme	molecules	603:611	arg1	characterization					577:592	the extensive characterization	563:592	the extensive characterization of rhEPO molecules	563:611	As described here, multiple complementary analytical approaches are utilized for the extensive characterization of rhEPO molecules, and more importantly for the structural comparison of the rhEPO analogues on the Chinese market.
32928385	3	72	used	utilized	550:557	arg2	approaches					535:544	multiple complementary analytical approaches	501:544	multiple complementary analytical approaches	501:544	As described here, multiple complementary analytical approaches are utilized for the extensive characterization of rhEPO molecules, and more importantly for the structural comparison of the rhEPO analogues on the Chinese market.
32928385	6	73	theme	sialic	1166:1171	arg1	acids					1173:1177	sialic acids	1166:1177	sialic acids	1166:1177	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	6	74	from	extension	1288:1296	arg1	acids					1273:1277	sialic acids	1266:1277	sialic acids	1266:1277	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	0	75	theme	human	63:67	arg1	rhEPO					85:89	rhEPO	85:89	rhEPO	85:89	UPLC-MS assessment on the structural similarity of recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring.
32928385	0	75	theme	human	63:67	arg1	erythropoietin					69:82	recombinant human erythropoietin	51:82	recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring	51:153	UPLC-MS assessment on the structural similarity of recombinant human erythropoietin (rhEPO) analogues from manufacturers in China for attribute monitoring.
32928385	6	76	theme	multi-antennary	1186:1200	arg1	structures					1202:1211	multi-antennary structures	1186:1211	multi-antennary structures	1186:1211	Each rhEPO product showed a characteristic glycoform profile with respect to the distribution of sialic acids across multi-antennary structures, the occurrence of O-glycosylation, O-acetylation on sialic acids, and the extension of N-glycan antennae with N-acetyllactosamine units.
32928385	3	77	from	market	703:708	arg1	comparison					654:663	the structural comparison	639:663	the structural comparison of the rhEPO analogues on the Chinese market	639:708	As described here, multiple complementary analytical approaches are utilized for the extensive characterization of rhEPO molecules, and more importantly for the structural comparison of the rhEPO analogues on the Chinese market.
32928385	1	78	theme	main	232:235	arg1	glycoprotein					202:213	a glycoprotein	200:213	a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy	200:357	Recombinant human erythropoietin (rhEPO) is a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy.
32928385	1	78	theme	main	232:235	arg1	hormone					237:243	the main hormone	228:243	the main hormone involved in regulating red blood cell production	228:292	Recombinant human erythropoietin (rhEPO) is a glycoprotein that acts as the main hormone involved in regulating red blood cell production to treat anemia caused by chronic kidney disease or chemotherapy.
33943034	8	0	theme	tissue	1463:1468	arg1	engineering					1470:1480	tissue engineering	1463:1480	tissue engineering	1463:1480	The high degree of discrete tuneability of composition and mechanics in these hydrogels makes them particularly promising for matching the chemical and mechanical requirements of different applications in tissue engineering and regenerative medicine.
33943034	3	1	theme	self-assembling	376:390	arg1	peptides					401:408	short self-assembling β-fibril peptides	370:408	short self-assembling β-fibril peptides	370:408	Here hybrid hydrogels are investigated formed from short self-assembling β-fibril peptides, and the glycosaminoglycan chondroitin sulfate (CS), chosen to replicate physical aspects of proteoglycans, specifically natural aggrecan, which provides structural mechanics to soft tissues.
33943034	4	2	theme	compositional	625:637	arg1	ratio					639:643	the peptide:CS compositional ratio	610:643	Varying the peptide:CS compositional ratio (1:2, 1:10, or 1:20)	602:664	Varying the peptide:CS compositional ratio (1:2, 1:10, or 1:20) can tune the mechanics of the gel by one to two orders of magnitude.
33943034	8	3	theme	regenerative	1486:1497	arg1	medicine					1499:1506	regenerative medicine	1486:1506	regenerative medicine	1486:1506	The high degree of discrete tuneability of composition and mechanics in these hydrogels makes them particularly promising for matching the chemical and mechanical requirements of different applications in tissue engineering and regenerative medicine.
33943034	4	4	theme	Varying	602:608	arg1	ratio					639:643	the peptide:CS compositional ratio	610:643	Varying the peptide:CS compositional ratio (1:2, 1:10, or 1:20)	602:664	Varying the peptide:CS compositional ratio (1:2, 1:10, or 1:20) can tune the mechanics of the gel by one to two orders of magnitude.
33943034	8	5	theme	mechanical	1410:1419	arg1	requirements					1421:1432	the chemical and mechanical requirements	1393:1432	the chemical and mechanical requirements of different applications	1393:1458	The high degree of discrete tuneability of composition and mechanics in these hydrogels makes them particularly promising for matching the chemical and mechanical requirements of different applications in tissue engineering and regenerative medicine.
33943034	4	6	theme	CS	622:623	arg1	ratio					639:643	the peptide:CS compositional ratio	610:643	Varying the peptide:CS compositional ratio (1:2, 1:10, or 1:20)	602:664	Varying the peptide:CS compositional ratio (1:2, 1:10, or 1:20) can tune the mechanics of the gel by one to two orders of magnitude.
33943034	7	7	theme	elastic	1156:1162	arg1	moduli					1164:1169	shear elastic moduli	1150:1169	shear elastic moduli from 0.14 to 220 kPa	1150:1190	The resulting hydrogels range in shear elastic moduli from 0.14 to 220 kPa, mimicking the mechanical variability in a range of soft tissues.
33943034	8	8	theme	tuneability	1286:1296	arg1	degree					1267:1272	The high degree	1258:1272	The high degree of discrete tuneability of composition and mechanics in these hydrogels	1258:1344	The high degree of discrete tuneability of composition and mechanics in these hydrogels makes them particularly promising for matching the chemical and mechanical requirements of different applications in tissue engineering and regenerative medicine.
33943034	7	9	theme	shear	1150:1154	arg1	moduli					1164:1169	shear elastic moduli	1150:1169	shear elastic moduli from 0.14 to 220 kPa	1150:1190	The resulting hydrogels range in shear elastic moduli from 0.14 to 220 kPa, mimicking the mechanical variability in a range of soft tissues.
33943034	6	10	dep	network	1001:1007	arg1	length					1024:1029	length	1024:1029	length	1024:1029	This tuneability arises due to changes in the mesoscale structure of the gel network (fibril width, length, and connectivity), giving rise to both shear-thickening and shear-thinning behavior.
33943034	6	10	dep	network	1001:1007	arg1	connectivity					1036:1047	connectivity	1036:1047	connectivity	1036:1047	This tuneability arises due to changes in the mesoscale structure of the gel network (fibril width, length, and connectivity), giving rise to both shear-thickening and shear-thinning behavior.
33943034	6	10	dep	network	1001:1007	arg1	width					1017:1021	fibril width	1010:1021	fibril width	1010:1021	This tuneability arises due to changes in the mesoscale structure of the gel network (fibril width, length, and connectivity), giving rise to both shear-thickening and shear-thinning behavior.
33943034	5	11	theme	magnitude	866:874	arg1	orders					856:861	approximately two orders	838:861	approximately two orders of magnitude	838:874	In addition, it is demonstrated that at any fixed composition, the gel shear modulus can be tuned over approximately two orders of magnitude through varying the initial vortex mixing time.
33943034	3	12	theme	natural	531:537	arg1	aggrecan					539:546	natural aggrecan	531:546	specifically natural aggrecan	518:546	Here hybrid hydrogels are investigated formed from short self-assembling β-fibril peptides, and the glycosaminoglycan chondroitin sulfate (CS), chosen to replicate physical aspects of proteoglycans, specifically natural aggrecan, which provides structural mechanics to soft tissues.
33943034	3	12	theme	natural	531:537	arg1	proteoglycans					503:515	proteoglycans	503:515	proteoglycans	503:515	Here hybrid hydrogels are investigated formed from short self-assembling β-fibril peptides, and the glycosaminoglycan chondroitin sulfate (CS), chosen to replicate physical aspects of proteoglycans, specifically natural aggrecan, which provides structural mechanics to soft tissues.
33943034	3	13	theme	β-fibril	392:399	arg1	peptides					401:408	short self-assembling β-fibril peptides	370:408	short self-assembling β-fibril peptides	370:408	Here hybrid hydrogels are investigated formed from short self-assembling β-fibril peptides, and the glycosaminoglycan chondroitin sulfate (CS), chosen to replicate physical aspects of proteoglycans, specifically natural aggrecan, which provides structural mechanics to soft tissues.
33943034	6	14	theme	shear-thickening	1071:1086	arg1	behavior					1107:1114	both shear-thickening and shear-thinning behavior	1066:1114	both shear-thickening and shear-thinning behavior	1066:1114	This tuneability arises due to changes in the mesoscale structure of the gel network (fibril width, length, and connectivity), giving rise to both shear-thickening and shear-thinning behavior.
33943034	7	15	theme	resulting	1121:1129	arg1	hydrogels					1131:1139	The resulting hydrogels	1117:1139	The resulting hydrogels	1117:1139	The resulting hydrogels range in shear elastic moduli from 0.14 to 220 kPa, mimicking the mechanical variability in a range of soft tissues.
33943034	6	16	theme	fibril	1010:1015	arg1	width					1017:1021	fibril width	1010:1021	fibril width	1010:1021	This tuneability arises due to changes in the mesoscale structure of the gel network (fibril width, length, and connectivity), giving rise to both shear-thickening and shear-thinning behavior.
33943034	1	17	theme	Self-assembling	86:100	arg1	hydrogels					102:110	Self-assembling hydrogels	86:110	Self-assembling hydrogels	86:110	Self-assembling hydrogels are promising materials for regenerative medicine and tissue engineering.
33943034	1	17	theme	Self-assembling	86:100	arg1	materials					126:134	promising materials	116:134	promising materials for regenerative medicine and tissue engineering	116:183	Self-assembling hydrogels are promising materials for regenerative medicine and tissue engineering.
33943034	2	18	from	order	238:242	arg1	mechanics					282:290	soft tissue mechanics	270:290	soft tissue mechanics	270:290	However, designing hydrogels that replicate the 3-4 order of magnitude variation in soft tissue mechanics remains a major challenge.
33943034	8	19	theme	high	1262:1265	arg1	degree					1267:1272	The high degree	1258:1272	The high degree of discrete tuneability of composition and mechanics in these hydrogels	1258:1344	The high degree of discrete tuneability of composition and mechanics in these hydrogels makes them particularly promising for matching the chemical and mechanical requirements of different applications in tissue engineering and regenerative medicine.
33943034	6	20	theme	mesoscale	970:978	arg1	structure					980:988	the mesoscale structure	966:988	the mesoscale structure of the gel network (fibril width, length, and connectivity)	966:1048	This tuneability arises due to changes in the mesoscale structure of the gel network (fibril width, length, and connectivity), giving rise to both shear-thickening and shear-thinning behavior.
33943034	7	21	from	variability	1218:1228	arg1	range					1235:1239	a range	1233:1239	a range of soft tissues	1233:1255	The resulting hydrogels range in shear elastic moduli from 0.14 to 220 kPa, mimicking the mechanical variability in a range of soft tissues.
33943034	2	22	theme	major	302:306	arg1	challenge					308:316	a major challenge	300:316	a major challenge	300:316	However, designing hydrogels that replicate the 3-4 order of magnitude variation in soft tissue mechanics remains a major challenge.
33943034	3	23	theme	physical	483:490	arg1	aspects					492:498	physical aspects	483:498	physical aspects of proteoglycans, specifically natural aggrecan	483:546	Here hybrid hydrogels are investigated formed from short self-assembling β-fibril peptides, and the glycosaminoglycan chondroitin sulfate (CS), chosen to replicate physical aspects of proteoglycans, specifically natural aggrecan, which provides structural mechanics to soft tissues.
33943034	3	24	theme	chondroitin	437:447	arg1	CS					458:459	CS	458:459	CS	458:459	Here hybrid hydrogels are investigated formed from short self-assembling β-fibril peptides, and the glycosaminoglycan chondroitin sulfate (CS), chosen to replicate physical aspects of proteoglycans, specifically natural aggrecan, which provides structural mechanics to soft tissues.
33943034	3	24	theme	chondroitin	437:447	arg1	sulfate					449:455	the glycosaminoglycan chondroitin sulfate	415:455	the glycosaminoglycan chondroitin sulfate (CS)	415:460	Here hybrid hydrogels are investigated formed from short self-assembling β-fibril peptides, and the glycosaminoglycan chondroitin sulfate (CS), chosen to replicate physical aspects of proteoglycans, specifically natural aggrecan, which provides structural mechanics to soft tissues.
33943034	7	25	from	range	1141:1145	arg1	moduli					1164:1169	shear elastic moduli	1150:1169	shear elastic moduli from 0.14 to 220 kPa	1150:1190	The resulting hydrogels range in shear elastic moduli from 0.14 to 220 kPa, mimicking the mechanical variability in a range of soft tissues.
33943034	7	25	from	range	1141:1145	arg1	220 kPa					1184:1190	220 kPa	1184:1190	220 kPa	1184:1190	The resulting hydrogels range in shear elastic moduli from 0.14 to 220 kPa, mimicking the mechanical variability in a range of soft tissues.
33943034	8	26	from	degree	1267:1272	arg1	hydrogels					1336:1344	these hydrogels	1330:1344	these hydrogels	1330:1344	The high degree of discrete tuneability of composition and mechanics in these hydrogels makes them particularly promising for matching the chemical and mechanical requirements of different applications in tissue engineering and regenerative medicine.
33943034	5	27	theme	mixing	911:916	arg1	time					918:921	the initial vortex mixing time	892:921	the initial vortex mixing time	892:921	In addition, it is demonstrated that at any fixed composition, the gel shear modulus can be tuned over approximately two orders of magnitude through varying the initial vortex mixing time.
33943034	0	28	theme	Hydrodynamic	0:11	arg1	Mixing					13:18	Hydrodynamic Mixing	0:18	Hydrodynamic Mixing	0:18	Hydrodynamic Mixing Tunes the Stiffness of Proteoglycan-Mimicking Physical Hydrogels.
33943034	7	29	from	220 kPa	1184:1190	arg1	moduli					1164:1169	shear elastic moduli	1150:1169	shear elastic moduli from 0.14 to 220 kPa	1150:1190	The resulting hydrogels range in shear elastic moduli from 0.14 to 220 kPa, mimicking the mechanical variability in a range of soft tissues.
33943034	7	29	from	220 kPa	1184:1190	arg1	range					1141:1145	range	1141:1145	range	1141:1145	The resulting hydrogels range in shear elastic moduli from 0.14 to 220 kPa, mimicking the mechanical variability in a range of soft tissues.
33943034	1	30	theme	promising	116:124	arg1	hydrogels					102:110	Self-assembling hydrogels	86:110	Self-assembling hydrogels	86:110	Self-assembling hydrogels are promising materials for regenerative medicine and tissue engineering.
33943034	1	30	theme	promising	116:124	arg1	materials					126:134	promising materials	116:134	promising materials for regenerative medicine and tissue engineering	116:183	Self-assembling hydrogels are promising materials for regenerative medicine and tissue engineering.
33943034	8	31	theme	chemical	1397:1404	arg1	requirements					1421:1432	the chemical and mechanical requirements	1393:1432	the chemical and mechanical requirements of different applications	1393:1458	The high degree of discrete tuneability of composition and mechanics in these hydrogels makes them particularly promising for matching the chemical and mechanical requirements of different applications in tissue engineering and regenerative medicine.
33943034	4	32	theme	magnitude	724:732	arg1	orders					714:719	one to two orders	703:719	one to two orders of magnitude	703:732	Varying the peptide:CS compositional ratio (1:2, 1:10, or 1:20) can tune the mechanics of the gel by one to two orders of magnitude.
33943034	5	33	theme	vortex	904:909	arg1	time					918:921	the initial vortex mixing time	892:921	the initial vortex mixing time	892:921	In addition, it is demonstrated that at any fixed composition, the gel shear modulus can be tuned over approximately two orders of magnitude through varying the initial vortex mixing time.
33943034	4	34	dep	two	710:712	arg1	to					707:708	to	707:708	to	707:708	Varying the peptide:CS compositional ratio (1:2, 1:10, or 1:20) can tune the mechanics of the gel by one to two orders of magnitude.
33943034	3	35	theme	proteoglycans	503:515	arg1	aspects					492:498	physical aspects	483:498	physical aspects of proteoglycans, specifically natural aggrecan	483:546	Here hybrid hydrogels are investigated formed from short self-assembling β-fibril peptides, and the glycosaminoglycan chondroitin sulfate (CS), chosen to replicate physical aspects of proteoglycans, specifically natural aggrecan, which provides structural mechanics to soft tissues.
33943034	3	36	theme	glycosaminoglycan	419:435	arg1	CS					458:459	CS	458:459	CS	458:459	Here hybrid hydrogels are investigated formed from short self-assembling β-fibril peptides, and the glycosaminoglycan chondroitin sulfate (CS), chosen to replicate physical aspects of proteoglycans, specifically natural aggrecan, which provides structural mechanics to soft tissues.
33943034	3	36	theme	glycosaminoglycan	419:435	arg1	sulfate					449:455	the glycosaminoglycan chondroitin sulfate	415:455	the glycosaminoglycan chondroitin sulfate (CS)	415:460	Here hybrid hydrogels are investigated formed from short self-assembling β-fibril peptides, and the glycosaminoglycan chondroitin sulfate (CS), chosen to replicate physical aspects of proteoglycans, specifically natural aggrecan, which provides structural mechanics to soft tissues.
33943034	8	37	theme	composition	1301:1311	arg1	tuneability					1286:1296	discrete tuneability	1277:1296	discrete tuneability of composition and mechanics	1277:1325	The high degree of discrete tuneability of composition and mechanics in these hydrogels makes them particularly promising for matching the chemical and mechanical requirements of different applications in tissue engineering and regenerative medicine.
33943034	8	38	theme	applications	1447:1458	arg1	requirements					1421:1432	the chemical and mechanical requirements	1393:1432	the chemical and mechanical requirements of different applications	1393:1458	The high degree of discrete tuneability of composition and mechanics in these hydrogels makes them particularly promising for matching the chemical and mechanical requirements of different applications in tissue engineering and regenerative medicine.
33943034	8	39	theme	mechanics	1317:1325	arg1	tuneability					1286:1296	discrete tuneability	1277:1296	discrete tuneability of composition and mechanics	1277:1325	The high degree of discrete tuneability of composition and mechanics in these hydrogels makes them particularly promising for matching the chemical and mechanical requirements of different applications in tissue engineering and regenerative medicine.
33943034	4	40	theme	gel	696:698	arg1	mechanics					679:687	the mechanics	675:687	the mechanics of the gel	675:698	Varying the peptide:CS compositional ratio (1:2, 1:10, or 1:20) can tune the mechanics of the gel by one to two orders of magnitude.
33943034	7	41	theme	soft	1244:1247	arg1	tissues					1249:1255	soft tissues	1244:1255	soft tissues	1244:1255	The resulting hydrogels range in shear elastic moduli from 0.14 to 220 kPa, mimicking the mechanical variability in a range of soft tissues.
33943034	4	42	theme	peptide	614:620	arg1	ratio					639:643	the peptide:CS compositional ratio	610:643	Varying the peptide:CS compositional ratio (1:2, 1:10, or 1:20)	602:664	Varying the peptide:CS compositional ratio (1:2, 1:10, or 1:20) can tune the mechanics of the gel by one to two orders of magnitude.
33943034	4	43	dep	ratio	639:643	arg1	1:10					651:654	1:10	651:654	1:10	651:654	Varying the peptide:CS compositional ratio (1:2, 1:10, or 1:20) can tune the mechanics of the gel by one to two orders of magnitude.
33943034	4	43	dep	ratio	639:643	arg1	1:20					660:663	1:20	660:663	1:20	660:663	Varying the peptide:CS compositional ratio (1:2, 1:10, or 1:20) can tune the mechanics of the gel by one to two orders of magnitude.
33943034	4	43	dep	ratio	639:643	arg1	1:2					646:648	1:2	646:648	1:2	646:648	Varying the peptide:CS compositional ratio (1:2, 1:10, or 1:20) can tune the mechanics of the gel by one to two orders of magnitude.
33943034	2	44	theme	soft	270:273	arg1	mechanics					282:290	soft tissue mechanics	270:290	soft tissue mechanics	270:290	However, designing hydrogels that replicate the 3-4 order of magnitude variation in soft tissue mechanics remains a major challenge.
33943034	7	45	dep	220 kPa	1184:1190	arg1	to					1181:1182	to	1181:1182	to	1181:1182	The resulting hydrogels range in shear elastic moduli from 0.14 to 220 kPa, mimicking the mechanical variability in a range of soft tissues.
33943034	3	46	dep	investigated	345:356	arg1	formed					358:363	formed	358:363	are investigated formed from short self-assembling β-fibril peptides, and the glycosaminoglycan chondroitin sulfate (CS), chosen to replicate physical aspects of proteoglycans, specifically natural aggrecan, which provides structural mechanics to soft tissues	341:599	Here hybrid hydrogels are investigated formed from short self-assembling β-fibril peptides, and the glycosaminoglycan chondroitin sulfate (CS), chosen to replicate physical aspects of proteoglycans, specifically natural aggrecan, which provides structural mechanics to soft tissues.
33943034	0	47	theme	Physical	66:73	arg1	Hydrogels					75:83	Proteoglycan-Mimicking Physical Hydrogels	43:83	Proteoglycan-Mimicking Physical Hydrogels	43:83	Hydrodynamic Mixing Tunes the Stiffness of Proteoglycan-Mimicking Physical Hydrogels.
33943034	5	48	theme	fixed	779:783	arg1	composition					785:795	any fixed composition	775:795	any fixed composition	775:795	In addition, it is demonstrated that at any fixed composition, the gel shear modulus can be tuned over approximately two orders of magnitude through varying the initial vortex mixing time.
33943034	2	49	theme	designing	195:203	arg1	hydrogels					205:213	designing hydrogels	195:213	designing hydrogels that replicate the 3-4 order of magnitude variation in soft tissue mechanics	195:290	However, designing hydrogels that replicate the 3-4 order of magnitude variation in soft tissue mechanics remains a major challenge.
33943034	2	50	theme	variation	257:265	arg1	order					238:242	the 3-4 order	230:242	the 3-4 order of magnitude variation in soft tissue mechanics	230:290	However, designing hydrogels that replicate the 3-4 order of magnitude variation in soft tissue mechanics remains a major challenge.
33943034	1	51	theme	regenerative	140:151	arg1	medicine					153:160	regenerative medicine	140:160	regenerative medicine	140:160	Self-assembling hydrogels are promising materials for regenerative medicine and tissue engineering.
33943034	0	52	theme	Proteoglycan-Mimicking	43:64	arg1	Hydrogels					75:83	Proteoglycan-Mimicking Physical Hydrogels	43:83	Proteoglycan-Mimicking Physical Hydrogels	43:83	Hydrodynamic Mixing Tunes the Stiffness of Proteoglycan-Mimicking Physical Hydrogels.
33943034	8	53	theme	different	1437:1445	arg1	applications					1447:1458	different applications	1437:1458	different applications	1437:1458	The high degree of discrete tuneability of composition and mechanics in these hydrogels makes them particularly promising for matching the chemical and mechanical requirements of different applications in tissue engineering and regenerative medicine.
33943034	2	54	theme	magnitude	247:255	arg1	variation					257:265	magnitude variation	247:265	magnitude variation	247:265	However, designing hydrogels that replicate the 3-4 order of magnitude variation in soft tissue mechanics remains a major challenge.
33943034	3	55	theme	hybrid	324:329	arg1	hydrogels					331:339	hybrid hydrogels	324:339	hybrid hydrogels	324:339	Here hybrid hydrogels are investigated formed from short self-assembling β-fibril peptides, and the glycosaminoglycan chondroitin sulfate (CS), chosen to replicate physical aspects of proteoglycans, specifically natural aggrecan, which provides structural mechanics to soft tissues.
33943034	5	56	theme	initial	896:902	arg1	time					918:921	the initial vortex mixing time	892:921	the initial vortex mixing time	892:921	In addition, it is demonstrated that at any fixed composition, the gel shear modulus can be tuned over approximately two orders of magnitude through varying the initial vortex mixing time.
33943034	6	57	theme	shear-thinning	1092:1105	arg1	behavior					1107:1114	both shear-thickening and shear-thinning behavior	1066:1114	both shear-thickening and shear-thinning behavior	1066:1114	This tuneability arises due to changes in the mesoscale structure of the gel network (fibril width, length, and connectivity), giving rise to both shear-thickening and shear-thinning behavior.
33943034	6	58	theme	gel	997:999	arg1	network					1001:1007	the gel network	993:1007	the gel network (fibril width, length, and connectivity)	993:1048	This tuneability arises due to changes in the mesoscale structure of the gel network (fibril width, length, and connectivity), giving rise to both shear-thickening and shear-thinning behavior.
33943034	3	59	theme	soft	588:591	arg1	tissues					593:599	soft tissues	588:599	soft tissues	588:599	Here hybrid hydrogels are investigated formed from short self-assembling β-fibril peptides, and the glycosaminoglycan chondroitin sulfate (CS), chosen to replicate physical aspects of proteoglycans, specifically natural aggrecan, which provides structural mechanics to soft tissues.
33943034	2	60	theme	3-4	234:236	arg1	order					238:242	the 3-4 order	230:242	the 3-4 order of magnitude variation in soft tissue mechanics	230:290	However, designing hydrogels that replicate the 3-4 order of magnitude variation in soft tissue mechanics remains a major challenge.
33943034	5	61	theme	gel	802:804	arg1	modulus					812:818	the gel shear modulus	798:818	the gel shear modulus	798:818	In addition, it is demonstrated that at any fixed composition, the gel shear modulus can be tuned over approximately two orders of magnitude through varying the initial vortex mixing time.
33943034	1	62	theme	tissue	166:171	arg1	engineering					173:183	tissue engineering	166:183	tissue engineering	166:183	Self-assembling hydrogels are promising materials for regenerative medicine and tissue engineering.
33943034	8	63	theme	discrete	1277:1284	arg1	tuneability					1286:1296	discrete tuneability	1277:1296	discrete tuneability of composition and mechanics	1277:1325	The high degree of discrete tuneability of composition and mechanics in these hydrogels makes them particularly promising for matching the chemical and mechanical requirements of different applications in tissue engineering and regenerative medicine.
33943034	6	64	from	changes	955:961	arg1	structure					980:988	the mesoscale structure	966:988	the mesoscale structure of the gel network (fibril width, length, and connectivity)	966:1048	This tuneability arises due to changes in the mesoscale structure of the gel network (fibril width, length, and connectivity), giving rise to both shear-thickening and shear-thinning behavior.
33943034	3	65	theme	short	370:374	arg1	peptides					401:408	short self-assembling β-fibril peptides	370:408	short self-assembling β-fibril peptides	370:408	Here hybrid hydrogels are investigated formed from short self-assembling β-fibril peptides, and the glycosaminoglycan chondroitin sulfate (CS), chosen to replicate physical aspects of proteoglycans, specifically natural aggrecan, which provides structural mechanics to soft tissues.
33943034	6	66	theme	network	1001:1007	arg1	structure					980:988	the mesoscale structure	966:988	the mesoscale structure of the gel network (fibril width, length, and connectivity)	966:1048	This tuneability arises due to changes in the mesoscale structure of the gel network (fibril width, length, and connectivity), giving rise to both shear-thickening and shear-thinning behavior.
33943034	5	67	theme	shear	806:810	arg1	modulus					812:818	the gel shear modulus	798:818	the gel shear modulus	798:818	In addition, it is demonstrated that at any fixed composition, the gel shear modulus can be tuned over approximately two orders of magnitude through varying the initial vortex mixing time.
33943034	3	68	theme	structural	564:573	arg1	mechanics					575:583	structural mechanics	564:583	structural mechanics	564:583	Here hybrid hydrogels are investigated formed from short self-assembling β-fibril peptides, and the glycosaminoglycan chondroitin sulfate (CS), chosen to replicate physical aspects of proteoglycans, specifically natural aggrecan, which provides structural mechanics to soft tissues.
33943034	7	69	theme	tissues	1249:1255	arg1	range					1235:1239	a range	1233:1239	a range of soft tissues	1233:1255	The resulting hydrogels range in shear elastic moduli from 0.14 to 220 kPa, mimicking the mechanical variability in a range of soft tissues.
33943034	0	70	theme	Hydrogels	75:83	arg1	Stiffness					30:38	the Stiffness	26:38	the Stiffness of Proteoglycan-Mimicking Physical Hydrogels	26:83	Hydrodynamic Mixing Tunes the Stiffness of Proteoglycan-Mimicking Physical Hydrogels.
33943034	2	71	theme	tissue	275:280	arg1	mechanics					282:290	soft tissue mechanics	270:290	soft tissue mechanics	270:290	However, designing hydrogels that replicate the 3-4 order of magnitude variation in soft tissue mechanics remains a major challenge.
33943034	7	72	theme	mechanical	1207:1216	arg1	variability					1218:1228	the mechanical variability	1203:1228	the mechanical variability in a range of soft tissues	1203:1255	The resulting hydrogels range in shear elastic moduli from 0.14 to 220 kPa, mimicking the mechanical variability in a range of soft tissues.
32512581	7	0	theme	high	1150:1153	arg1	level					1155:1159	The high level	1146:1159	The high level of Actinobacteria	1146:1177	The high level of Actinobacteria aggravated the disorder of the intestinal flora and contributed to the progression from obesity to nonalcoholic steatohepatitis (NASH) and related diseases.
32512581	0	1	theme	NASH	82:85	arg1	progression					67:77	the progression	63:77	the progression of NASH	63:85	Polysaccharides isolated from Cordyceps Sinensis contribute to the progression of NASH by modifying the gut microbiota in mice fed a high-fat diet.
32512581	4	2	theme	HFD-induced	803:813	arg1	obesity					815:821	HFD-induced obesity	803:821	HFD-induced obesity	803:821	The results revealed that even though CSP supplementation could prevent an increase in body weight, it aggravated liver fibrosis and steatosis as evidenced by increased inflammation, lipid metabolism markers, insulin resistance (IR) and alanine aminotransferase (ALT) in HFD-induced obesity.
32512581	1	3	theme	Various	148:154	arg1	fibers					164:169	Various dietary fibers	148:169	Various dietary fibers	148:169	Various dietary fibers are considered to prevent obesity by modulating the gut microbiota.
32512581	4	4	theme	insulin	741:747	arg1	IR					761:762	IR	761:762	IR	761:762	The results revealed that even though CSP supplementation could prevent an increase in body weight, it aggravated liver fibrosis and steatosis as evidenced by increased inflammation, lipid metabolism markers, insulin resistance (IR) and alanine aminotransferase (ALT) in HFD-induced obesity.
32512581	4	4	theme	insulin	741:747	arg1	resistance					749:758	insulin resistance	741:758	insulin resistance (IR)	741:763	The results revealed that even though CSP supplementation could prevent an increase in body weight, it aggravated liver fibrosis and steatosis as evidenced by increased inflammation, lipid metabolism markers, insulin resistance (IR) and alanine aminotransferase (ALT) in HFD-induced obesity.
32512581	0	5	attach	isolated	16:23	arg2	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides isolated from Cordyceps Sinensis	0:47	Polysaccharides isolated from Cordyceps Sinensis contribute to the progression of NASH by modifying the gut microbiota in mice fed a high-fat diet.
32512581	0	5	attach	isolated	16:23	arg1	Sinensis					40:47	Cordyceps Sinensis	30:47	Cordyceps Sinensis	30:47	Polysaccharides isolated from Cordyceps Sinensis contribute to the progression of NASH by modifying the gut microbiota in mice fed a high-fat diet.
32512581	4	6	theme	metabolism	721:730	arg1	markers					732:738	lipid metabolism markers	715:738	lipid metabolism markers	715:738	The results revealed that even though CSP supplementation could prevent an increase in body weight, it aggravated liver fibrosis and steatosis as evidenced by increased inflammation, lipid metabolism markers, insulin resistance (IR) and alanine aminotransferase (ALT) in HFD-induced obesity.
32512581	5	7	theme	gut	873:875	arg1	composition					888:898	the gut microbiota composition	869:898	the gut microbiota composition	869:898	16S rDNA gene sequencing was used to analyze the gut microbiota composition, and the relative abundance of the Actinobacteria phylum, including the Olsenella genus, was significantly higher in CSP-treated mice than in HFD-fed mice.
32512581	4	8	theme	lipid	715:719	arg1	markers					732:738	lipid metabolism markers	715:738	lipid metabolism markers	715:738	The results revealed that even though CSP supplementation could prevent an increase in body weight, it aggravated liver fibrosis and steatosis as evidenced by increased inflammation, lipid metabolism markers, insulin resistance (IR) and alanine aminotransferase (ALT) in HFD-induced obesity.
32512581	7	9	theme	intestinal	1210:1219	arg1	flora					1221:1225	the intestinal flora	1206:1225	the intestinal flora	1206:1225	The high level of Actinobacteria aggravated the disorder of the intestinal flora and contributed to the progression from obesity to nonalcoholic steatohepatitis (NASH) and related diseases.
32512581	4	10	theme	liver	646:650	arg1	fibrosis					652:659	liver fibrosis	646:659	liver fibrosis	646:659	The results revealed that even though CSP supplementation could prevent an increase in body weight, it aggravated liver fibrosis and steatosis as evidenced by increased inflammation, lipid metabolism markers, insulin resistance (IR) and alanine aminotransferase (ALT) in HFD-induced obesity.
32512581	7	11	theme	flora	1221:1225	arg1	disorder					1194:1201	the disorder	1190:1201	the disorder of the intestinal flora	1190:1225	The high level of Actinobacteria aggravated the disorder of the intestinal flora and contributed to the progression from obesity to nonalcoholic steatohepatitis (NASH) and related diseases.
32512581	3	12	theme	diet	499:502	arg1	mice					526:529	high-fat diet (HFD)-feding C57BL/6J mice	490:529	high-fat diet (HFD)-feding C57BL/6J mice	490:529	The function and safety of CSP were tested in high-fat diet (HFD)-feding C57BL/6J mice.
32512581	1	13	theme	dietary	156:162	arg1	fibers					164:169	Various dietary fibers	148:169	Various dietary fibers	148:169	Various dietary fibers are considered to prevent obesity by modulating the gut microbiota.
32512581	0	14	theme	gut	104:106	arg1	microbiota					108:117	the gut microbiota	100:117	the gut microbiota in mice fed a high-fat diet	100:145	Polysaccharides isolated from Cordyceps Sinensis contribute to the progression of NASH by modifying the gut microbiota in mice fed a high-fat diet.
32512581	5	15	theme	HFD-fed	1042:1048	arg1	mice					1050:1053	HFD-fed mice	1042:1053	HFD-fed mice	1042:1053	16S rDNA gene sequencing was used to analyze the gut microbiota composition, and the relative abundance of the Actinobacteria phylum, including the Olsenella genus, was significantly higher in CSP-treated mice than in HFD-fed mice.
32512581	6	16	theme	Actinobacteria	1107:1120	arg1	proportion					1093:1102	the proportion	1089:1102	the proportion of Actinobacteria, which can degrade CSP	1089:1143	CSP supplementation may increase the proportion of Actinobacteria, which can degrade CSP.
32512581	7	17	theme	nonalcoholic	1278:1289	arg1	NASH					1308:1311	NASH	1308:1311	NASH	1308:1311	The high level of Actinobacteria aggravated the disorder of the intestinal flora and contributed to the progression from obesity to nonalcoholic steatohepatitis (NASH) and related diseases.
32512581	7	17	theme	nonalcoholic	1278:1289	arg1	steatohepatitis					1291:1305	nonalcoholic steatohepatitis	1278:1305	nonalcoholic steatohepatitis (NASH)	1278:1312	The high level of Actinobacteria aggravated the disorder of the intestinal flora and contributed to the progression from obesity to nonalcoholic steatohepatitis (NASH) and related diseases.
32512581	4	18	from	inflammation	701:712	arg1	obesity					815:821	HFD-induced obesity	803:821	HFD-induced obesity	803:821	The results revealed that even though CSP supplementation could prevent an increase in body weight, it aggravated liver fibrosis and steatosis as evidenced by increased inflammation, lipid metabolism markers, insulin resistance (IR) and alanine aminotransferase (ALT) in HFD-induced obesity.
32512581	4	19	theme	increased	691:699	arg1	inflammation					701:712	inflammation	701:712	inflammation	701:712	The results revealed that even though CSP supplementation could prevent an increase in body weight, it aggravated liver fibrosis and steatosis as evidenced by increased inflammation, lipid metabolism markers, insulin resistance (IR) and alanine aminotransferase (ALT) in HFD-induced obesity.
32512581	2	20	theme	dietary	292:298	arg1	polysaccharide					258:271	Cordyceps sinensis polysaccharide	239:271	Cordyceps sinensis polysaccharide (CSP)	239:277	Cordyceps sinensis polysaccharide (CSP) is a soluble dietary fiber known to have protective effects against obesity and related diseases, but whether these effects induce any side effects remains unknown.
32512581	2	20	theme	dietary	292:298	arg1	fiber					300:304	a soluble dietary fiber	282:304	a soluble dietary fiber known to have protective effects against obesity and related diseases	282:374	Cordyceps sinensis polysaccharide (CSP) is a soluble dietary fiber known to have protective effects against obesity and related diseases, but whether these effects induce any side effects remains unknown.
32512581	4	21	from	increase	607:614	arg1	weight					624:629	body weight	619:629	body weight	619:629	The results revealed that even though CSP supplementation could prevent an increase in body weight, it aggravated liver fibrosis and steatosis as evidenced by increased inflammation, lipid metabolism markers, insulin resistance (IR) and alanine aminotransferase (ALT) in HFD-induced obesity.
32512581	2	22	theme	related	359:365	arg1	diseases					367:374	related diseases	359:374	related diseases	359:374	Cordyceps sinensis polysaccharide (CSP) is a soluble dietary fiber known to have protective effects against obesity and related diseases, but whether these effects induce any side effects remains unknown.
32512581	0	23	theme	Cordyceps	30:38	arg1	Sinensis					40:47	Cordyceps Sinensis	30:47	Cordyceps Sinensis	30:47	Polysaccharides isolated from Cordyceps Sinensis contribute to the progression of NASH by modifying the gut microbiota in mice fed a high-fat diet.
32512581	5	24	theme	Actinobacteria	935:948	arg1	phylum					950:955	the Actinobacteria phylum	931:955	the Actinobacteria phylum	931:955	16S rDNA gene sequencing was used to analyze the gut microbiota composition, and the relative abundance of the Actinobacteria phylum, including the Olsenella genus, was significantly higher in CSP-treated mice than in HFD-fed mice.
32512581	5	25	theme	Olsenella	972:980	arg1	genus					982:986	the Olsenella genus	968:986	the Olsenella genus	968:986	16S rDNA gene sequencing was used to analyze the gut microbiota composition, and the relative abundance of the Actinobacteria phylum, including the Olsenella genus, was significantly higher in CSP-treated mice than in HFD-fed mice.
32512581	3	26	theme	-feding	509:515	arg1	mice					526:529	high-fat diet (HFD)-feding C57BL/6J mice	490:529	high-fat diet (HFD)-feding C57BL/6J mice	490:529	The function and safety of CSP were tested in high-fat diet (HFD)-feding C57BL/6J mice.
32512581	5	27	theme	microbiota	877:886	arg1	composition					888:898	the gut microbiota composition	869:898	the gut microbiota composition	869:898	16S rDNA gene sequencing was used to analyze the gut microbiota composition, and the relative abundance of the Actinobacteria phylum, including the Olsenella genus, was significantly higher in CSP-treated mice than in HFD-fed mice.
32512581	5	28	theme	relative	909:916	arg1	higher					1007:1012	higher	1007:1012	higher	1007:1012	16S rDNA gene sequencing was used to analyze the gut microbiota composition, and the relative abundance of the Actinobacteria phylum, including the Olsenella genus, was significantly higher in CSP-treated mice than in HFD-fed mice.
32512581	5	28	theme	relative	909:916	arg1	abundance					918:926	the relative abundance	905:926	the relative abundance of the Actinobacteria phylum, including the Olsenella genus,	905:987	16S rDNA gene sequencing was used to analyze the gut microbiota composition, and the relative abundance of the Actinobacteria phylum, including the Olsenella genus, was significantly higher in CSP-treated mice than in HFD-fed mice.
32512581	3	29	theme	C57BL/6J	517:524	arg1	mice					526:529	high-fat diet (HFD)-feding C57BL/6J mice	490:529	high-fat diet (HFD)-feding C57BL/6J mice	490:529	The function and safety of CSP were tested in high-fat diet (HFD)-feding C57BL/6J mice.
32512581	5	30	theme	phylum	950:955	arg1	higher					1007:1012	higher	1007:1012	higher	1007:1012	16S rDNA gene sequencing was used to analyze the gut microbiota composition, and the relative abundance of the Actinobacteria phylum, including the Olsenella genus, was significantly higher in CSP-treated mice than in HFD-fed mice.
32512581	5	30	theme	phylum	950:955	arg1	abundance					918:926	the relative abundance	905:926	the relative abundance of the Actinobacteria phylum, including the Olsenella genus,	905:987	16S rDNA gene sequencing was used to analyze the gut microbiota composition, and the relative abundance of the Actinobacteria phylum, including the Olsenella genus, was significantly higher in CSP-treated mice than in HFD-fed mice.
32512581	5	31	theme	16S	824:826	arg1	sequencing					838:847	16S rDNA gene sequencing	824:847	16S rDNA gene sequencing	824:847	16S rDNA gene sequencing was used to analyze the gut microbiota composition, and the relative abundance of the Actinobacteria phylum, including the Olsenella genus, was significantly higher in CSP-treated mice than in HFD-fed mice.
32512581	4	32	theme	body	619:622	arg1	weight					624:629	body weight	619:629	body weight	619:629	The results revealed that even though CSP supplementation could prevent an increase in body weight, it aggravated liver fibrosis and steatosis as evidenced by increased inflammation, lipid metabolism markers, insulin resistance (IR) and alanine aminotransferase (ALT) in HFD-induced obesity.
32512581	5	33	theme	CSP-treated	1017:1027	arg1	mice					1029:1032	CSP-treated mice	1017:1032	CSP-treated mice	1017:1032	16S rDNA gene sequencing was used to analyze the gut microbiota composition, and the relative abundance of the Actinobacteria phylum, including the Olsenella genus, was significantly higher in CSP-treated mice than in HFD-fed mice.
32512581	2	34	theme	side	414:417	arg1	effects					419:425	any side effects	410:425	any side effects	410:425	Cordyceps sinensis polysaccharide (CSP) is a soluble dietary fiber known to have protective effects against obesity and related diseases, but whether these effects induce any side effects remains unknown.
32512581	4	35	from	aminotransferase	777:792	arg1	obesity					815:821	HFD-induced obesity	803:821	HFD-induced obesity	803:821	The results revealed that even though CSP supplementation could prevent an increase in body weight, it aggravated liver fibrosis and steatosis as evidenced by increased inflammation, lipid metabolism markers, insulin resistance (IR) and alanine aminotransferase (ALT) in HFD-induced obesity.
32512581	2	36	theme	protective	320:329	arg1	effects					331:337	protective effects	320:337	protective effects	320:337	Cordyceps sinensis polysaccharide (CSP) is a soluble dietary fiber known to have protective effects against obesity and related diseases, but whether these effects induce any side effects remains unknown.
32512581	0	37	theme	high-fat	133:140	arg1	diet					142:145	a high-fat diet	131:145	a high-fat diet	131:145	Polysaccharides isolated from Cordyceps Sinensis contribute to the progression of NASH by modifying the gut microbiota in mice fed a high-fat diet.
32512581	0	38	from	microbiota	108:117	arg1	mice					122:125	mice	122:125	mice fed a high-fat diet	122:145	Polysaccharides isolated from Cordyceps Sinensis contribute to the progression of NASH by modifying the gut microbiota in mice fed a high-fat diet.
32512581	4	39	theme	alanine	769:775	arg1	ALT					795:797	ALT	795:797	ALT	795:797	The results revealed that even though CSP supplementation could prevent an increase in body weight, it aggravated liver fibrosis and steatosis as evidenced by increased inflammation, lipid metabolism markers, insulin resistance (IR) and alanine aminotransferase (ALT) in HFD-induced obesity.
32512581	4	39	theme	alanine	769:775	arg1	aminotransferase					777:792	alanine aminotransferase	769:792	alanine aminotransferase (ALT)	769:798	The results revealed that even though CSP supplementation could prevent an increase in body weight, it aggravated liver fibrosis and steatosis as evidenced by increased inflammation, lipid metabolism markers, insulin resistance (IR) and alanine aminotransferase (ALT) in HFD-induced obesity.
32512581	3	40	theme	CSP	471:473	arg1	safety					461:466	safety	461:466	safety	461:466	The function and safety of CSP were tested in high-fat diet (HFD)-feding C57BL/6J mice.
32512581	3	40	theme	CSP	471:473	arg1	function					448:455	function	448:455	function	448:455	The function and safety of CSP were tested in high-fat diet (HFD)-feding C57BL/6J mice.
32512581	5	41	used	used	853:856	arg2	sequencing					838:847	16S rDNA gene sequencing	824:847	16S rDNA gene sequencing	824:847	16S rDNA gene sequencing was used to analyze the gut microbiota composition, and the relative abundance of the Actinobacteria phylum, including the Olsenella genus, was significantly higher in CSP-treated mice than in HFD-fed mice.
32512581	4	42	from	markers	732:738	arg1	obesity					815:821	HFD-induced obesity	803:821	HFD-induced obesity	803:821	The results revealed that even though CSP supplementation could prevent an increase in body weight, it aggravated liver fibrosis and steatosis as evidenced by increased inflammation, lipid metabolism markers, insulin resistance (IR) and alanine aminotransferase (ALT) in HFD-induced obesity.
32512581	6	43	theme	CSP	1056:1058	arg1	supplementation					1060:1074	CSP supplementation	1056:1074	CSP supplementation	1056:1074	CSP supplementation may increase the proportion of Actinobacteria, which can degrade CSP.
32512581	2	44	theme	soluble	284:290	arg1	polysaccharide					258:271	Cordyceps sinensis polysaccharide	239:271	Cordyceps sinensis polysaccharide (CSP)	239:277	Cordyceps sinensis polysaccharide (CSP) is a soluble dietary fiber known to have protective effects against obesity and related diseases, but whether these effects induce any side effects remains unknown.
32512581	2	44	theme	soluble	284:290	arg1	fiber					300:304	a soluble dietary fiber	282:304	a soluble dietary fiber known to have protective effects against obesity and related diseases	282:374	Cordyceps sinensis polysaccharide (CSP) is a soluble dietary fiber known to have protective effects against obesity and related diseases, but whether these effects induce any side effects remains unknown.
32512581	2	45	theme	sinensis	249:256	arg1	fiber					300:304	a soluble dietary fiber	282:304	a soluble dietary fiber known to have protective effects against obesity and related diseases	282:374	Cordyceps sinensis polysaccharide (CSP) is a soluble dietary fiber known to have protective effects against obesity and related diseases, but whether these effects induce any side effects remains unknown.
32512581	2	45	theme	sinensis	249:256	arg1	CSP					274:276	CSP	274:276	CSP	274:276	Cordyceps sinensis polysaccharide (CSP) is a soluble dietary fiber known to have protective effects against obesity and related diseases, but whether these effects induce any side effects remains unknown.
32512581	2	45	theme	sinensis	249:256	arg1	polysaccharide					258:271	Cordyceps sinensis polysaccharide	239:271	Cordyceps sinensis polysaccharide (CSP)	239:277	Cordyceps sinensis polysaccharide (CSP) is a soluble dietary fiber known to have protective effects against obesity and related diseases, but whether these effects induce any side effects remains unknown.
32512581	5	46	theme	gene	833:836	arg1	sequencing					838:847	16S rDNA gene sequencing	824:847	16S rDNA gene sequencing	824:847	16S rDNA gene sequencing was used to analyze the gut microbiota composition, and the relative abundance of the Actinobacteria phylum, including the Olsenella genus, was significantly higher in CSP-treated mice than in HFD-fed mice.
32512581	2	47	theme	Cordyceps	239:247	arg1	fiber					300:304	a soluble dietary fiber	282:304	a soluble dietary fiber known to have protective effects against obesity and related diseases	282:374	Cordyceps sinensis polysaccharide (CSP) is a soluble dietary fiber known to have protective effects against obesity and related diseases, but whether these effects induce any side effects remains unknown.
32512581	2	47	theme	Cordyceps	239:247	arg1	CSP					274:276	CSP	274:276	CSP	274:276	Cordyceps sinensis polysaccharide (CSP) is a soluble dietary fiber known to have protective effects against obesity and related diseases, but whether these effects induce any side effects remains unknown.
32512581	2	47	theme	Cordyceps	239:247	arg1	polysaccharide					258:271	Cordyceps sinensis polysaccharide	239:271	Cordyceps sinensis polysaccharide (CSP)	239:277	Cordyceps sinensis polysaccharide (CSP) is a soluble dietary fiber known to have protective effects against obesity and related diseases, but whether these effects induce any side effects remains unknown.
32512581	7	48	from	obesity	1267:1273	arg1	progression					1250:1260	the progression	1246:1260	the progression from obesity to nonalcoholic steatohepatitis (NASH) and related diseases	1246:1333	The high level of Actinobacteria aggravated the disorder of the intestinal flora and contributed to the progression from obesity to nonalcoholic steatohepatitis (NASH) and related diseases.
32512581	5	49	theme	rDNA	828:831	arg1	sequencing					838:847	16S rDNA gene sequencing	824:847	16S rDNA gene sequencing	824:847	16S rDNA gene sequencing was used to analyze the gut microbiota composition, and the relative abundance of the Actinobacteria phylum, including the Olsenella genus, was significantly higher in CSP-treated mice than in HFD-fed mice.
32512581	1	50	theme	gut	223:225	arg1	microbiota					227:236	the gut microbiota	219:236	the gut microbiota	219:236	Various dietary fibers are considered to prevent obesity by modulating the gut microbiota.
32512581	7	51	theme	Actinobacteria	1164:1177	arg1	level					1155:1159	The high level	1146:1159	The high level of Actinobacteria	1146:1177	The high level of Actinobacteria aggravated the disorder of the intestinal flora and contributed to the progression from obesity to nonalcoholic steatohepatitis (NASH) and related diseases.
32512581	3	52	dep	function	448:455	arg1	The					444:446	The	444:446	The	444:446	The function and safety of CSP were tested in high-fat diet (HFD)-feding C57BL/6J mice.
32512581	4	53	from	resistance	749:758	arg1	obesity					815:821	HFD-induced obesity	803:821	HFD-induced obesity	803:821	The results revealed that even though CSP supplementation could prevent an increase in body weight, it aggravated liver fibrosis and steatosis as evidenced by increased inflammation, lipid metabolism markers, insulin resistance (IR) and alanine aminotransferase (ALT) in HFD-induced obesity.
32512581	3	54	theme	high-fat	490:497	arg1	HFD					505:507	HFD	505:507	HFD	505:507	The function and safety of CSP were tested in high-fat diet (HFD)-feding C57BL/6J mice.
32512581	3	54	theme	high-fat	490:497	arg1	diet					499:502	high-fat diet	490:502	high-fat diet (HFD)-feding C57BL/6J mice	490:529	The function and safety of CSP were tested in high-fat diet (HFD)-feding C57BL/6J mice.
32512581	7	55	theme	related	1318:1324	arg1	diseases					1326:1333	related diseases	1318:1333	related diseases	1318:1333	The high level of Actinobacteria aggravated the disorder of the intestinal flora and contributed to the progression from obesity to nonalcoholic steatohepatitis (NASH) and related diseases.
32512581	4	56	theme	CSP	570:572	arg1	supplementation					574:588	CSP supplementation	570:588	CSP supplementation	570:588	The results revealed that even though CSP supplementation could prevent an increase in body weight, it aggravated liver fibrosis and steatosis as evidenced by increased inflammation, lipid metabolism markers, insulin resistance (IR) and alanine aminotransferase (ALT) in HFD-induced obesity.
34838829	2	0	theme	biocompatible	461:473	arg1	matrix					484:489	the biocompatible hydrogel matrix	457:489	the biocompatible hydrogel matrix	457:489	In the current work, we have prepared sodium alginate (SAlg), dextrin, and acrylic acid (AA) based cross-linked hydrogels, composed of bentonite incorporated in the biocompatible hydrogel matrix.
34838829	4	1	theme	infra-red	691:699	arg1	spectroscopy					701:712	infra-red spectroscopy	691:712	infra-red spectroscopy (FTIR)	691:719	As-synthesised hydrogel (SAlg/dextrin-cl-PAA) and hydrogel composite (SAlg/dextrin-cl-PAA/bentonite) were further analysed by infra-red spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and thermogravimetric analysis (TGA/DSC).
34838829	4	1	theme	infra-red	691:699	arg1	FTIR					715:718	FTIR	715:718	FTIR	715:718	As-synthesised hydrogel (SAlg/dextrin-cl-PAA) and hydrogel composite (SAlg/dextrin-cl-PAA/bentonite) were further analysed by infra-red spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and thermogravimetric analysis (TGA/DSC).
34838829	10	2	theme	synthesised	1496:1506	arg1	adsorbents					1508:1517	the synthesised adsorbents	1492:1517	the synthesised adsorbents	1492:1517	The adsorption results demonstrate that the synthesised adsorbents are very efficient for removing herbicides (PQ) from wastewater.
34838829	10	2	theme	synthesised	1496:1506	arg1	efficient					1528:1536	efficient	1528:1536	efficient	1528:1536	The adsorption results demonstrate that the synthesised adsorbents are very efficient for removing herbicides (PQ) from wastewater.
34838829	9	3	contain	possess	1389:1395	arg2	reproducibility					1402:1416	good reproducibility	1397:1416	good reproducibility	1397:1416	Moreover, reusability analysis shows that the adsorbents possess good reproducibility even after six successive cycles.
34838829	9	3	contain	possess	1389:1395	arg1	adsorbents					1378:1387	the adsorbents	1374:1387	the adsorbents	1374:1387	Moreover, reusability analysis shows that the adsorbents possess good reproducibility even after six successive cycles.
34838829	0	4	theme	facile	99:104	arg1	removal					106:112	facile removal	99:112	facile removal of paraquat herbicide from aqueous solutions	99:157	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel nanohybrids for facile removal of paraquat herbicide from aqueous solutions.
34838829	4	5	theme	electron	771:778	arg1	FESEM					792:796	FESEM	792:796	FESEM	792:796	As-synthesised hydrogel (SAlg/dextrin-cl-PAA) and hydrogel composite (SAlg/dextrin-cl-PAA/bentonite) were further analysed by infra-red spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and thermogravimetric analysis (TGA/DSC).
34838829	4	5	theme	electron	771:778	arg1	microscopy					780:789	field emission scanning electron microscopy	747:789	field emission scanning electron microscopy (FESEM)	747:797	As-synthesised hydrogel (SAlg/dextrin-cl-PAA) and hydrogel composite (SAlg/dextrin-cl-PAA/bentonite) were further analysed by infra-red spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and thermogravimetric analysis (TGA/DSC).
34838829	2	6	link	cross-linked	395:406	arg1	hydrogels					408:416	cross-linked hydrogels	395:416	cross-linked hydrogels	395:416	In the current work, we have prepared sodium alginate (SAlg), dextrin, and acrylic acid (AA) based cross-linked hydrogels, composed of bentonite incorporated in the biocompatible hydrogel matrix.
34838829	2	7	theme	current	303:309	arg1	work					311:314	the current work	299:314	the current work	299:314	In the current work, we have prepared sodium alginate (SAlg), dextrin, and acrylic acid (AA) based cross-linked hydrogels, composed of bentonite incorporated in the biocompatible hydrogel matrix.
34838829	1	8	theme	wider	278:282	arg1	population					284:293	the wider population	274:293	the wider population	274:293	Removal of hazardous herbicides from the aqueous solution is critical for overcoming health-related issues across the wider population.
34838829	3	9	theme	toxic	534:538	arg1	paraquat					550:557	highly toxic herbicide paraquat	527:557	highly toxic herbicide paraquat (PQ)	527:562	This hydrogel composite can remove highly toxic herbicide paraquat (PQ).
34838829	3	9	theme	toxic	534:538	arg1	PQ					560:561	PQ	560:561	PQ	560:561	This hydrogel composite can remove highly toxic herbicide paraquat (PQ).
34838829	0	10	from	solutions	149:157	arg1	removal					106:112	facile removal	99:112	facile removal of paraquat herbicide from aqueous solutions	99:157	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel nanohybrids for facile removal of paraquat herbicide from aqueous solutions.
34838829	9	11	theme	successive	1433:1442	arg1	cycles					1444:1449	six successive cycles	1429:1449	six successive cycles	1429:1449	Moreover, reusability analysis shows that the adsorbents possess good reproducibility even after six successive cycles.
34838829	7	12	theme	Langmuir	1111:1118	arg1	isotherm					1120:1127	Langmuir isotherm	1111:1127	Langmuir isotherm	1111:1127	Pseudo-second-order (PSO) and Langmuir isotherm models have shown to be best suited for accurately describing the adsorption mechanism.
34838829	4	13	theme	scanning	762:769	arg1	FESEM					792:796	FESEM	792:796	FESEM	792:796	As-synthesised hydrogel (SAlg/dextrin-cl-PAA) and hydrogel composite (SAlg/dextrin-cl-PAA/bentonite) were further analysed by infra-red spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and thermogravimetric analysis (TGA/DSC).
34838829	4	13	theme	scanning	762:769	arg1	microscopy					780:789	field emission scanning electron microscopy	747:789	field emission scanning electron microscopy (FESEM)	747:797	As-synthesised hydrogel (SAlg/dextrin-cl-PAA) and hydrogel composite (SAlg/dextrin-cl-PAA/bentonite) were further analysed by infra-red spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and thermogravimetric analysis (TGA/DSC).
34838829	5	14	theme	dextrin-based	891:903	arg1	hydrogel					905:912	dextrin-based hydrogel	891:912	dextrin-based hydrogel	891:912	For the first time, PQ adsorption onto sodium and dextrin-based hydrogel was also evaluated.
34838829	1	15	theme	hazardous	171:179	arg1	herbicides					181:190	hazardous herbicides	171:190	hazardous herbicides	171:190	Removal of hazardous herbicides from the aqueous solution is critical for overcoming health-related issues across the wider population.
34838829	4	16	theme	As-synthesised	565:578	arg1	SAlg/dextrin-cl-PAA					590:608	SAlg/dextrin-cl-PAA	590:608	SAlg/dextrin-cl-PAA	590:608	As-synthesised hydrogel (SAlg/dextrin-cl-PAA) and hydrogel composite (SAlg/dextrin-cl-PAA/bentonite) were further analysed by infra-red spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and thermogravimetric analysis (TGA/DSC).
34838829	4	16	theme	As-synthesised	565:578	arg1	hydrogel					580:587	As-synthesised hydrogel	565:587	As-synthesised hydrogel (SAlg/dextrin-cl-PAA)	565:609	As-synthesised hydrogel (SAlg/dextrin-cl-PAA) and hydrogel composite (SAlg/dextrin-cl-PAA/bentonite) were further analysed by infra-red spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and thermogravimetric analysis (TGA/DSC).
34838829	6	17	dep	SAlg/dextrin-cl-PAA	1012:1030	arg1	the					1008:1010	the	1008:1010	the	1008:1010	The measured highest removal capacities were 76.923 and 90.909 mg g-1 for the SAlg/dextrin-cl-PAA and SAlg/dextrin-cl-PAA/bentonite, respectively.
34838829	7	18	theme	isotherm	1120:1127	arg1	models					1129:1134	Pseudo-second-order (PSO) and Langmuir isotherm models	1081:1134	Pseudo-second-order (PSO) and Langmuir isotherm models	1081:1134	Pseudo-second-order (PSO) and Langmuir isotherm models have shown to be best suited for accurately describing the adsorption mechanism.
34838829	4	19	theme	emission	753:760	arg1	FESEM					792:796	FESEM	792:796	FESEM	792:796	As-synthesised hydrogel (SAlg/dextrin-cl-PAA) and hydrogel composite (SAlg/dextrin-cl-PAA/bentonite) were further analysed by infra-red spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and thermogravimetric analysis (TGA/DSC).
34838829	4	19	theme	emission	753:760	arg1	microscopy					780:789	field emission scanning electron microscopy	747:789	field emission scanning electron microscopy (FESEM)	747:797	As-synthesised hydrogel (SAlg/dextrin-cl-PAA) and hydrogel composite (SAlg/dextrin-cl-PAA/bentonite) were further analysed by infra-red spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and thermogravimetric analysis (TGA/DSC).
34838829	1	20	theme	herbicides	181:190	arg1	Removal					160:166	Removal	160:166	Removal of hazardous herbicides from the aqueous solution	160:216	Removal of hazardous herbicides from the aqueous solution is critical for overcoming health-related issues across the wider population.
34838829	0	21	theme	sodium	16:21	arg1	hydrogel					74:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel	0:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel	0:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel nanohybrids for facile removal of paraquat herbicide from aqueous solutions.
34838829	10	22	theme	adsorption	1456:1465	arg1	results					1467:1473	The adsorption results	1452:1473	The adsorption results	1452:1473	The adsorption results demonstrate that the synthesised adsorbents are very efficient for removing herbicides (PQ) from wastewater.
34838829	5	23	theme	first	849:853	arg1	time					855:858	the first time	845:858	the first time	845:858	For the first time, PQ adsorption onto sodium and dextrin-based hydrogel was also evaluated.
34838829	0	24	theme	Bentonite-based	0:14	arg1	hydrogel					74:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel	0:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel	0:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel nanohybrids for facile removal of paraquat herbicide from aqueous solutions.
34838829	6	25	theme	removal	955:961	arg1	76.923					979:984	76.923	979:984	76.923	979:984	The measured highest removal capacities were 76.923 and 90.909 mg g-1 for the SAlg/dextrin-cl-PAA and SAlg/dextrin-cl-PAA/bentonite, respectively.
34838829	6	25	theme	removal	955:961	arg1	capacities					963:972	highest removal capacities	947:972	The measured highest removal capacities	934:972	The measured highest removal capacities were 76.923 and 90.909 mg g-1 for the SAlg/dextrin-cl-PAA and SAlg/dextrin-cl-PAA/bentonite, respectively.
34838829	2	26	dep	prepared	325:332	arg1	based					389:393	based	389:393	based cross-linked hydrogels, composed of bentonite incorporated in the biocompatible hydrogel matrix	389:489	In the current work, we have prepared sodium alginate (SAlg), dextrin, and acrylic acid (AA) based cross-linked hydrogels, composed of bentonite incorporated in the biocompatible hydrogel matrix.
34838829	8	27	theme	PQ	1272:1273	arg1	adsorption					1258:1267	the adsorption	1254:1267	the adsorption of PQ on adsorbents	1254:1287	A thermodynamics study verified that the adsorption of PQ on adsorbents is spontaneous, favourable and exothermic.
34838829	8	27	theme	PQ	1272:1273	arg1	spontaneous					1292:1302	spontaneous	1292:1302	spontaneous	1292:1302	A thermodynamics study verified that the adsorption of PQ on adsorbents is spontaneous, favourable and exothermic.
34838829	0	28	theme	dextrin	33:39	arg1	hydrogel					74:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel	0:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel	0:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel nanohybrids for facile removal of paraquat herbicide from aqueous solutions.
34838829	6	29	theme	highest	947:953	arg1	76.923					979:984	76.923	979:984	76.923	979:984	The measured highest removal capacities were 76.923 and 90.909 mg g-1 for the SAlg/dextrin-cl-PAA and SAlg/dextrin-cl-PAA/bentonite, respectively.
34838829	6	29	theme	highest	947:953	arg1	capacities					963:972	highest removal capacities	947:972	The measured highest removal capacities	934:972	The measured highest removal capacities were 76.923 and 90.909 mg g-1 for the SAlg/dextrin-cl-PAA and SAlg/dextrin-cl-PAA/bentonite, respectively.
34838829	0	30	theme	herbicide	126:134	arg1	removal					106:112	facile removal	99:112	facile removal of paraquat herbicide from aqueous solutions	99:157	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel nanohybrids for facile removal of paraquat herbicide from aqueous solutions.
34838829	0	31	link	cross-linked	41:52	arg1	hydrogel					74:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel	0:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel	0:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel nanohybrids for facile removal of paraquat herbicide from aqueous solutions.
34838829	3	32	theme	herbicide	540:548	arg1	paraquat					550:557	highly toxic herbicide paraquat	527:557	highly toxic herbicide paraquat (PQ)	527:562	This hydrogel composite can remove highly toxic herbicide paraquat (PQ).
34838829	3	32	theme	herbicide	540:548	arg1	PQ					560:561	PQ	560:561	PQ	560:561	This hydrogel composite can remove highly toxic herbicide paraquat (PQ).
34838829	1	33	theme	aqueous	201:207	arg1	solution					209:216	the aqueous solution	197:216	the aqueous solution	197:216	Removal of hazardous herbicides from the aqueous solution is critical for overcoming health-related issues across the wider population.
34838829	0	34	theme	alginate/	23:31	arg1	hydrogel					74:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel	0:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel	0:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel nanohybrids for facile removal of paraquat herbicide from aqueous solutions.
34838829	6	35	theme	measured	938:945	arg1	76.923					979:984	76.923	979:984	76.923	979:984	The measured highest removal capacities were 76.923 and 90.909 mg g-1 for the SAlg/dextrin-cl-PAA and SAlg/dextrin-cl-PAA/bentonite, respectively.
34838829	6	35	theme	measured	938:945	arg1	capacities					963:972	highest removal capacities	947:972	The measured highest removal capacities	934:972	The measured highest removal capacities were 76.923 and 90.909 mg g-1 for the SAlg/dextrin-cl-PAA and SAlg/dextrin-cl-PAA/bentonite, respectively.
34838829	0	36	theme	paraquat	117:124	arg1	herbicide					126:134	paraquat herbicide	117:134	paraquat herbicide	117:134	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel nanohybrids for facile removal of paraquat herbicide from aqueous solutions.
34838829	4	37	theme	hydrogel	615:622	arg1	SAlg/dextrin-cl-PAA/bentonite					635:663	SAlg/dextrin-cl-PAA/bentonite	635:663	SAlg/dextrin-cl-PAA/bentonite	635:663	As-synthesised hydrogel (SAlg/dextrin-cl-PAA) and hydrogel composite (SAlg/dextrin-cl-PAA/bentonite) were further analysed by infra-red spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and thermogravimetric analysis (TGA/DSC).
34838829	4	37	theme	hydrogel	615:622	arg1	composite					624:632	hydrogel composite	615:632	hydrogel composite (SAlg/dextrin-cl-PAA/bentonite)	615:664	As-synthesised hydrogel (SAlg/dextrin-cl-PAA) and hydrogel composite (SAlg/dextrin-cl-PAA/bentonite) were further analysed by infra-red spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and thermogravimetric analysis (TGA/DSC).
34838829	0	38	theme	poly	54:57	arg1	hydrogel					74:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel	0:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel	0:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel nanohybrids for facile removal of paraquat herbicide from aqueous solutions.
34838829	0	39	theme	aqueous	141:147	arg1	solutions					149:157	aqueous solutions	141:157	aqueous solutions	141:157	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel nanohybrids for facile removal of paraquat herbicide from aqueous solutions.
34838829	7	40	theme	adsorption	1195:1204	arg1	mechanism					1206:1214	the adsorption mechanism	1191:1214	the adsorption mechanism	1191:1214	Pseudo-second-order (PSO) and Langmuir isotherm models have shown to be best suited for accurately describing the adsorption mechanism.
34838829	0	41	theme	cross-linked	41:52	arg1	hydrogel					74:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel	0:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel	0:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel nanohybrids for facile removal of paraquat herbicide from aqueous solutions.
34838829	7	42	theme	Pseudo-second-order	1081:1099	arg1	models					1129:1134	Pseudo-second-order (PSO) and Langmuir isotherm models	1081:1134	Pseudo-second-order (PSO) and Langmuir isotherm models	1081:1134	Pseudo-second-order (PSO) and Langmuir isotherm models have shown to be best suited for accurately describing the adsorption mechanism.
34838829	8	43	theme	thermodynamics	1219:1232	arg1	study					1234:1238	A thermodynamics study	1217:1238	A thermodynamics study	1217:1238	A thermodynamics study verified that the adsorption of PQ on adsorbents is spontaneous, favourable and exothermic.
34838829	0	44	theme	acrylic	60:66	arg1	poly					54:57	poly	54:57	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel	0:81	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel nanohybrids for facile removal of paraquat herbicide from aqueous solutions.
34838829	0	44	theme	acrylic	60:66	arg1	acid					68:71	acrylic acid	60:71	acrylic acid	60:71	Bentonite-based sodium alginate/ dextrin cross-linked poly (acrylic acid) hydrogel nanohybrids for facile removal of paraquat herbicide from aqueous solutions.
34838829	9	45	theme	good	1397:1400	arg1	reproducibility					1402:1416	good reproducibility	1397:1416	good reproducibility	1397:1416	Moreover, reusability analysis shows that the adsorbents possess good reproducibility even after six successive cycles.
34838829	9	46	theme	reusability	1342:1352	arg1	analysis					1354:1361	reusability analysis	1342:1361	reusability analysis	1342:1361	Moreover, reusability analysis shows that the adsorbents possess good reproducibility even after six successive cycles.
34838829	4	47	theme	thermogravimetric	803:819	arg1	TGA/DSC					831:837	TGA/DSC	831:837	TGA/DSC	831:837	As-synthesised hydrogel (SAlg/dextrin-cl-PAA) and hydrogel composite (SAlg/dextrin-cl-PAA/bentonite) were further analysed by infra-red spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and thermogravimetric analysis (TGA/DSC).
34838829	4	47	theme	thermogravimetric	803:819	arg1	analysis					821:828	thermogravimetric analysis	803:828	thermogravimetric analysis (TGA/DSC)	803:838	As-synthesised hydrogel (SAlg/dextrin-cl-PAA) and hydrogel composite (SAlg/dextrin-cl-PAA/bentonite) were further analysed by infra-red spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and thermogravimetric analysis (TGA/DSC).
34838829	1	48	from	solution	209:216	arg1	Removal					160:166	Removal	160:166	Removal of hazardous herbicides from the aqueous solution	160:216	Removal of hazardous herbicides from the aqueous solution is critical for overcoming health-related issues across the wider population.
34838829	2	49	theme	sodium	334:339	arg1	alginate					341:348	sodium alginate	334:348	sodium alginate (SAlg)	334:355	In the current work, we have prepared sodium alginate (SAlg), dextrin, and acrylic acid (AA) based cross-linked hydrogels, composed of bentonite incorporated in the biocompatible hydrogel matrix.
34838829	2	49	theme	sodium	334:339	arg1	SAlg					351:354	SAlg	351:354	SAlg	351:354	In the current work, we have prepared sodium alginate (SAlg), dextrin, and acrylic acid (AA) based cross-linked hydrogels, composed of bentonite incorporated in the biocompatible hydrogel matrix.
34838829	8	50	from	adsorption	1258:1267	arg1	adsorbents					1278:1287	adsorbents	1278:1287	adsorbents	1278:1287	A thermodynamics study verified that the adsorption of PQ on adsorbents is spontaneous, favourable and exothermic.
34838829	4	51	theme	field	747:751	arg1	FESEM					792:796	FESEM	792:796	FESEM	792:796	As-synthesised hydrogel (SAlg/dextrin-cl-PAA) and hydrogel composite (SAlg/dextrin-cl-PAA/bentonite) were further analysed by infra-red spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and thermogravimetric analysis (TGA/DSC).
34838829	4	51	theme	field	747:751	arg1	microscopy					780:789	field emission scanning electron microscopy	747:789	field emission scanning electron microscopy (FESEM)	747:797	As-synthesised hydrogel (SAlg/dextrin-cl-PAA) and hydrogel composite (SAlg/dextrin-cl-PAA/bentonite) were further analysed by infra-red spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and thermogravimetric analysis (TGA/DSC).
34838829	4	52	theme	X-ray	722:726	arg1	XRD					741:743	XRD	741:743	XRD	741:743	As-synthesised hydrogel (SAlg/dextrin-cl-PAA) and hydrogel composite (SAlg/dextrin-cl-PAA/bentonite) were further analysed by infra-red spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and thermogravimetric analysis (TGA/DSC).
34838829	4	52	theme	X-ray	722:726	arg1	diffraction					728:738	X-ray diffraction	722:738	X-ray diffraction (XRD)	722:744	As-synthesised hydrogel (SAlg/dextrin-cl-PAA) and hydrogel composite (SAlg/dextrin-cl-PAA/bentonite) were further analysed by infra-red spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and thermogravimetric analysis (TGA/DSC).
34838829	3	53	theme	hydrogel	497:504	arg1	composite					506:514	This hydrogel composite	492:514	This hydrogel composite	492:514	This hydrogel composite can remove highly toxic herbicide paraquat (PQ).
34838829	2	54	theme	acrylic	371:377	arg1	AA					385:386	AA	385:386	AA	385:386	In the current work, we have prepared sodium alginate (SAlg), dextrin, and acrylic acid (AA) based cross-linked hydrogels, composed of bentonite incorporated in the biocompatible hydrogel matrix.
34838829	2	54	theme	acrylic	371:377	arg1	acid					379:382	acrylic acid	371:382	acrylic acid (AA)	371:387	In the current work, we have prepared sodium alginate (SAlg), dextrin, and acrylic acid (AA) based cross-linked hydrogels, composed of bentonite incorporated in the biocompatible hydrogel matrix.
34838829	1	55	theme	health-related	245:258	arg1	issues					260:265	health-related issues	245:265	health-related issues	245:265	Removal of hazardous herbicides from the aqueous solution is critical for overcoming health-related issues across the wider population.
34838829	2	56	theme	hydrogel	475:482	arg1	matrix					484:489	the biocompatible hydrogel matrix	457:489	the biocompatible hydrogel matrix	457:489	In the current work, we have prepared sodium alginate (SAlg), dextrin, and acrylic acid (AA) based cross-linked hydrogels, composed of bentonite incorporated in the biocompatible hydrogel matrix.
34838829	2	57	theme	cross-linked	395:406	arg1	hydrogels					408:416	cross-linked hydrogels	395:416	cross-linked hydrogels	395:416	In the current work, we have prepared sodium alginate (SAlg), dextrin, and acrylic acid (AA) based cross-linked hydrogels, composed of bentonite incorporated in the biocompatible hydrogel matrix.
34838829	5	58	theme	PQ	861:862	arg1	adsorption					864:873	PQ adsorption	861:873	PQ adsorption onto sodium and dextrin-based hydrogel	861:912	For the first time, PQ adsorption onto sodium and dextrin-based hydrogel was also evaluated.
33155575	4	0	theme	mAh	643:645	arg1	g-1					647:649	869 mAh g-1	639:649	869 mAh g-1	639:649	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	4	1	from	g-1	647:649	arg1	g-1					703:705	0.2 A g-1	697:705	0.2 A g-1	697:705	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	6	2	theme	structural	1104:1113	arg1	stability					1115:1123	the structural stability	1100:1123	the structural stability	1100:1123	Its excellent properties and stability are attributed to the abundant porosity of the carbon in the starch derived layer, which improves the structural stability and electrochemical kinetics.
33155575	4	3	theme	g-1	647:649	arg1	capacity					627:634	a high initial capacity	612:634	a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1	612:705	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	5	4	theme	current	878:884	arg1	density					886:892	a current density	876:892	a current density of 0.2 A g-1	876:905	When the silicon content reaches 30%, the reversible capacity of the composite is 1490 mAh g-1 at a current density of 0.2 A g-1, and the capacity remains 850 mAh g-1 after 100 cycles.
33155575	6	5	theme	electrochemical	1129:1143	arg1	kinetics					1145:1152	electrochemical kinetics	1129:1152	electrochemical kinetics	1129:1152	Its excellent properties and stability are attributed to the abundant porosity of the carbon in the starch derived layer, which improves the structural stability and electrochemical kinetics.
33155575	4	6	from	g-1	703:705	arg1	g-1					647:649	869 mAh g-1	639:649	869 mAh g-1	639:649	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	4	6	from	g-1	703:705	arg1	%					692:692	66%	690:692	66% at 0.2 A g-1	690:705	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	4	6	from	g-1	703:705	arg1	efficiency					676:685	an initial Coulombic efficiency	655:685	an initial Coulombic efficiency of 66% at 0.2 A g-1	655:705	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	4	7	theme	A	701:701	arg1	g-1					703:705	0.2 A g-1	697:705	0.2 A g-1	697:705	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	4	8	theme	composite	564:572	arg1	material					574:581	the anode composite material	554:581	the anode composite material with 20% silicon content	554:606	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	5	9	theme	mAh	865:867	arg1	g-1					869:871	1490 mAh g-1	860:871	1490 mAh g-1	860:871	When the silicon content reaches 30%, the reversible capacity of the composite is 1490 mAh g-1 at a current density of 0.2 A g-1, and the capacity remains 850 mAh g-1 after 100 cycles.
33155575	5	9	theme	mAh	865:867	arg1	capacity					831:838	the reversible capacity	816:838	the reversible capacity of the composite	816:855	When the silicon content reaches 30%, the reversible capacity of the composite is 1490 mAh g-1 at a current density of 0.2 A g-1, and the capacity remains 850 mAh g-1 after 100 cycles.
33155575	3	10	theme	silicon	523:529	arg1	expansion					510:518	the volume expansion	499:518	the volume expansion of silicon	499:529	A PS based carbon/silicon/carbon composite was prepared by coating and carbonizing the starch slurry, which can alleviate the volume expansion of silicon.
33155575	5	11	from	density	886:892	arg1	g-1					869:871	1490 mAh g-1	860:871	1490 mAh g-1	860:871	When the silicon content reaches 30%, the reversible capacity of the composite is 1490 mAh g-1 at a current density of 0.2 A g-1, and the capacity remains 850 mAh g-1 after 100 cycles.
33155575	5	11	from	density	886:892	arg1	capacity					831:838	the reversible capacity	816:838	the reversible capacity of the composite	816:855	When the silicon content reaches 30%, the reversible capacity of the composite is 1490 mAh g-1 at a current density of 0.2 A g-1, and the capacity remains 850 mAh g-1 after 100 cycles.
33155575	4	12	theme	anode	558:562	arg1	material					574:581	the anode composite material	554:581	the anode composite material with 20% silicon content	554:606	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	4	13	theme	initial	658:664	arg1	efficiency					676:685	an initial Coulombic efficiency	655:685	an initial Coulombic efficiency of 66% at 0.2 A g-1	655:705	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	1	14	theme	Silicon/carbon	90:103	arg1	composites					105:114	Silicon/carbon composites	90:114	Silicon/carbon composites	90:114	Silicon/carbon composites have the disadvantages of large volume expansion and high cost, which limits their commercial application.
33155575	3	15	theme	carbon/silicon/carbon	388:408	arg1	composite					410:418	A PS based carbon/silicon/carbon composite	377:418	A PS based carbon/silicon/carbon composite	377:418	A PS based carbon/silicon/carbon composite was prepared by coating and carbonizing the starch slurry, which can alleviate the volume expansion of silicon.
33155575	4	16	theme	initial	619:625	arg1	capacity					627:634	a high initial capacity	612:634	a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1	612:705	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	0	17	theme	lithium-ion	44:54	arg1	composite					79:87	starch-based lithium-ion battery silicon-carbon composite	31:87	starch-based lithium-ion battery silicon-carbon composite	31:87	Biological enzyme treatment of starch-based lithium-ion battery silicon-carbon composite.
33155575	6	18	theme	abundant	1024:1031	arg1	porosity					1033:1040	the abundant porosity	1020:1040	the abundant porosity of the carbon in the starch derived layer, which improves the structural stability and electrochemical kinetics	1020:1152	Its excellent properties and stability are attributed to the abundant porosity of the carbon in the starch derived layer, which improves the structural stability and electrochemical kinetics.
33155575	2	19	used	used	268:271	arg2	economic					248:255	economic	248:255	economic	248:255	In this study, green and economic starch was used to prepare porous starch (PS) under the action of enzymes, and then nano-silica was embedded in the PS.
33155575	2	19	used	used	268:271	arg2	green					238:242	green	238:242	green	238:242	In this study, green and economic starch was used to prepare porous starch (PS) under the action of enzymes, and then nano-silica was embedded in the PS.
33155575	4	20	theme	high	614:617	arg1	capacity					627:634	a high initial capacity	612:634	a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1	612:705	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	0	21	theme	enzyme	11:16	arg1	treatment					18:26	Biological enzyme treatment	0:26	Biological enzyme treatment of starch-based lithium-ion battery silicon-carbon composite.	0:88	Biological enzyme treatment of starch-based lithium-ion battery silicon-carbon composite.
33155575	3	22	theme	volume	503:508	arg1	expansion					510:518	the volume expansion	499:518	the volume expansion of silicon	499:529	A PS based carbon/silicon/carbon composite was prepared by coating and carbonizing the starch slurry, which can alleviate the volume expansion of silicon.
33155575	1	23	theme	commercial	199:208	arg1	application					210:220	their commercial application	193:220	their commercial application	193:220	Silicon/carbon composites have the disadvantages of large volume expansion and high cost, which limits their commercial application.
33155575	0	24	theme	Biological	0:9	arg1	treatment					18:26	Biological enzyme treatment	0:26	Biological enzyme treatment of starch-based lithium-ion battery silicon-carbon composite.	0:88	Biological enzyme treatment of starch-based lithium-ion battery silicon-carbon composite.
33155575	4	25	theme	Coulombic	666:674	arg1	efficiency					676:685	an initial Coulombic efficiency	655:685	an initial Coulombic efficiency of 66% at 0.2 A g-1	655:705	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	5	26	theme	silicon	787:793	arg1	content					795:801	the silicon content	783:801	the silicon content	783:801	When the silicon content reaches 30%, the reversible capacity of the composite is 1490 mAh g-1 at a current density of 0.2 A g-1, and the capacity remains 850 mAh g-1 after 100 cycles.
33155575	7	27	theme	lithium-ion	1240:1250	arg1	materials					1266:1274	lithium-ion battery anode materials	1240:1274	lithium-ion battery anode materials	1240:1274	This method provides a reference for the sustainable and environmental protection of lithium-ion battery anode materials.
33155575	6	28	link	derived	1070:1076	arg1	layer					1078:1082	the starch derived layer	1059:1082	the starch derived layer	1059:1082	Its excellent properties and stability are attributed to the abundant porosity of the carbon in the starch derived layer, which improves the structural stability and electrochemical kinetics.
33155575	4	29	with	material	574:581	arg1	content					600:606	20% silicon content	588:606	20% silicon content	588:606	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	5	30	theme	composite	847:855	arg1	g-1					869:871	1490 mAh g-1	860:871	1490 mAh g-1	860:871	When the silicon content reaches 30%, the reversible capacity of the composite is 1490 mAh g-1 at a current density of 0.2 A g-1, and the capacity remains 850 mAh g-1 after 100 cycles.
33155575	5	30	theme	composite	847:855	arg1	capacity					831:838	the reversible capacity	816:838	the reversible capacity of the composite	816:855	When the silicon content reaches 30%, the reversible capacity of the composite is 1490 mAh g-1 at a current density of 0.2 A g-1, and the capacity remains 850 mAh g-1 after 100 cycles.
33155575	2	31	dep	green	238:242	arg1	starch					257:262	starch	257:262	starch	257:262	In this study, green and economic starch was used to prepare porous starch (PS) under the action of enzymes, and then nano-silica was embedded in the PS.
33155575	4	32	dep	g-1	756:758	arg1	cycles					770:775	100 cycles	766:775	100 cycles	766:775	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	2	33	theme	porous	284:289	arg1	starch					291:296	porous starch	284:296	porous starch (PS) under the action of enzymes	284:329	In this study, green and economic starch was used to prepare porous starch (PS) under the action of enzymes, and then nano-silica was embedded in the PS.
33155575	2	33	theme	porous	284:289	arg1	PS					299:300	PS	299:300	PS	299:300	In this study, green and economic starch was used to prepare porous starch (PS) under the action of enzymes, and then nano-silica was embedded in the PS.
33155575	4	34	contain	has	608:610	arg2	capacity					627:634	a high initial capacity	612:634	a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1	612:705	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	4	34	contain	has	608:610	arg1	material					574:581	the anode composite material	554:581	the anode composite material with 20% silicon content	554:606	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	4	35	theme	%	692:692	arg1	g-1					647:649	869 mAh g-1	639:649	869 mAh g-1	639:649	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	4	35	theme	%	692:692	arg1	efficiency					676:685	an initial Coulombic efficiency	655:685	an initial Coulombic efficiency of 66% at 0.2 A g-1	655:705	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	5	36	theme	g-1	903:905	arg1	density					886:892	a current density	876:892	a current density of 0.2 A g-1	876:905	When the silicon content reaches 30%, the reversible capacity of the composite is 1490 mAh g-1 at a current density of 0.2 A g-1, and the capacity remains 850 mAh g-1 after 100 cycles.
33155575	4	37	theme	silicon	592:598	arg1	content					600:606	20% silicon content	588:606	20% silicon content	588:606	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	3	38	theme	PS	379:380	arg1	composite					410:418	A PS based carbon/silicon/carbon composite	377:418	A PS based carbon/silicon/carbon composite	377:418	A PS based carbon/silicon/carbon composite was prepared by coating and carbonizing the starch slurry, which can alleviate the volume expansion of silicon.
33155575	2	39	theme	enzymes	323:329	arg1	action					313:318	the action	309:318	the action of enzymes	309:329	In this study, green and economic starch was used to prepare porous starch (PS) under the action of enzymes, and then nano-silica was embedded in the PS.
33155575	6	40	from	porosity	1033:1040	arg1	layer					1078:1082	the starch derived layer	1059:1082	the starch derived layer	1059:1082	Its excellent properties and stability are attributed to the abundant porosity of the carbon in the starch derived layer, which improves the structural stability and electrochemical kinetics.
33155575	5	41	theme	mAh	937:939	arg1	g-1					941:943	850 mAh g-1	933:943	850 mAh g-1 after 100 cycles	933:960	When the silicon content reaches 30%, the reversible capacity of the composite is 1490 mAh g-1 at a current density of 0.2 A g-1, and the capacity remains 850 mAh g-1 after 100 cycles.
33155575	7	42	theme	anode	1260:1264	arg1	materials					1266:1274	lithium-ion battery anode materials	1240:1274	lithium-ion battery anode materials	1240:1274	This method provides a reference for the sustainable and environmental protection of lithium-ion battery anode materials.
33155575	6	43	theme	carbon	1049:1054	arg1	porosity					1033:1040	the abundant porosity	1020:1040	the abundant porosity of the carbon in the starch derived layer, which improves the structural stability and electrochemical kinetics	1020:1152	Its excellent properties and stability are attributed to the abundant porosity of the carbon in the starch derived layer, which improves the structural stability and electrochemical kinetics.
33155575	0	44	theme	starch-based	31:42	arg1	composite					79:87	starch-based lithium-ion battery silicon-carbon composite	31:87	starch-based lithium-ion battery silicon-carbon composite	31:87	Biological enzyme treatment of starch-based lithium-ion battery silicon-carbon composite.
33155575	3	45	theme	based	382:386	arg1	composite					410:418	A PS based carbon/silicon/carbon composite	377:418	A PS based carbon/silicon/carbon composite	377:418	A PS based carbon/silicon/carbon composite was prepared by coating and carbonizing the starch slurry, which can alleviate the volume expansion of silicon.
33155575	5	46	theme	A	901:901	arg1	g-1					903:905	0.2 A g-1	897:905	0.2 A g-1	897:905	When the silicon content reaches 30%, the reversible capacity of the composite is 1490 mAh g-1 at a current density of 0.2 A g-1, and the capacity remains 850 mAh g-1 after 100 cycles.
33155575	7	47	theme	battery	1252:1258	arg1	materials					1266:1274	lithium-ion battery anode materials	1240:1274	lithium-ion battery anode materials	1240:1274	This method provides a reference for the sustainable and environmental protection of lithium-ion battery anode materials.
33155575	0	48	theme	silicon-carbon	64:77	arg1	composite					79:87	starch-based lithium-ion battery silicon-carbon composite	31:87	starch-based lithium-ion battery silicon-carbon composite	31:87	Biological enzyme treatment of starch-based lithium-ion battery silicon-carbon composite.
33155575	6	49	dep	derived	1070:1076	arg1	starch					1063:1068	starch	1063:1068	starch	1063:1068	Its excellent properties and stability are attributed to the abundant porosity of the carbon in the starch derived layer, which improves the structural stability and electrochemical kinetics.
33155575	4	50	theme	mAh	752:754	arg1	g-1					756:758	450 mAh g-1	748:758	450 mAh g-1	748:758	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	2	51	from	embedded	357:364	arg1	PS					373:374	the PS	369:374	the PS	369:374	In this study, green and economic starch was used to prepare porous starch (PS) under the action of enzymes, and then nano-silica was embedded in the PS.
33155575	1	52	theme	large	142:146	arg1	expansion					155:163	large volume expansion	142:163	large volume expansion	142:163	Silicon/carbon composites have the disadvantages of large volume expansion and high cost, which limits their commercial application.
33155575	0	53	theme	battery	56:62	arg1	composite					79:87	starch-based lithium-ion battery silicon-carbon composite	31:87	starch-based lithium-ion battery silicon-carbon composite	31:87	Biological enzyme treatment of starch-based lithium-ion battery silicon-carbon composite.
33155575	4	54	theme	450	748:750	arg1	mAh					752:754	mAh	752:754	mAh	752:754	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	2	55	from	PS	373:374	arg1	embedded					357:364	embedded	357:364	embedded	357:364	In this study, green and economic starch was used to prepare porous starch (PS) under the action of enzymes, and then nano-silica was embedded in the PS.
33155575	1	56	theme	volume	148:153	arg1	expansion					155:163	large volume expansion	142:163	large volume expansion	142:163	Silicon/carbon composites have the disadvantages of large volume expansion and high cost, which limits their commercial application.
33155575	4	57	from	efficiency	676:685	arg1	g-1					703:705	0.2 A g-1	697:705	0.2 A g-1	697:705	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	1	58	contain	have	116:119	arg1	composites					105:114	Silicon/carbon composites	90:114	Silicon/carbon composites	90:114	Silicon/carbon composites have the disadvantages of large volume expansion and high cost, which limits their commercial application.
33155575	1	58	contain	have	116:119	arg2	disadvantages					125:137	the disadvantages	121:137	the disadvantages	121:137	Silicon/carbon composites have the disadvantages of large volume expansion and high cost, which limits their commercial application.
33155575	7	59	theme	environmental	1212:1224	arg1	protection					1226:1235	the sustainable and environmental protection	1192:1235	the sustainable and environmental protection of lithium-ion battery anode materials	1192:1274	This method provides a reference for the sustainable and environmental protection of lithium-ion battery anode materials.
33155575	7	60	theme	materials	1266:1274	arg1	protection					1226:1235	the sustainable and environmental protection	1192:1235	the sustainable and environmental protection of lithium-ion battery anode materials	1192:1274	This method provides a reference for the sustainable and environmental protection of lithium-ion battery anode materials.
33155575	1	61	theme	expansion	155:163	arg1	disadvantages					125:137	the disadvantages	121:137	the disadvantages	121:137	Silicon/carbon composites have the disadvantages of large volume expansion and high cost, which limits their commercial application.
33155575	6	62	theme	excellent	967:975	arg1	properties					977:986	Its excellent properties	963:986	Its excellent properties	963:986	Its excellent properties and stability are attributed to the abundant porosity of the carbon in the starch derived layer, which improves the structural stability and electrochemical kinetics.
33155575	5	63	theme	reversible	820:829	arg1	g-1					869:871	1490 mAh g-1	860:871	1490 mAh g-1	860:871	When the silicon content reaches 30%, the reversible capacity of the composite is 1490 mAh g-1 at a current density of 0.2 A g-1, and the capacity remains 850 mAh g-1 after 100 cycles.
33155575	5	63	theme	reversible	820:829	arg1	capacity					831:838	the reversible capacity	816:838	the reversible capacity of the composite	816:855	When the silicon content reaches 30%, the reversible capacity of the composite is 1490 mAh g-1 at a current density of 0.2 A g-1, and the capacity remains 850 mAh g-1 after 100 cycles.
33155575	5	64	theme	1490	860:863	arg1	g-1					869:871	1490 mAh g-1	860:871	1490 mAh g-1	860:871	When the silicon content reaches 30%, the reversible capacity of the composite is 1490 mAh g-1 at a current density of 0.2 A g-1, and the capacity remains 850 mAh g-1 after 100 cycles.
33155575	5	64	theme	1490	860:863	arg1	capacity					831:838	the reversible capacity	816:838	the reversible capacity of the composite	816:855	When the silicon content reaches 30%, the reversible capacity of the composite is 1490 mAh g-1 at a current density of 0.2 A g-1, and the capacity remains 850 mAh g-1 after 100 cycles.
33155575	3	65	theme	starch	464:469	arg1	slurry					471:476	the starch slurry	460:476	the starch slurry	460:476	A PS based carbon/silicon/carbon composite was prepared by coating and carbonizing the starch slurry, which can alleviate the volume expansion of silicon.
33155575	4	66	theme	efficiency	676:685	arg1	capacity					627:634	a high initial capacity	612:634	a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1	612:705	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	1	67	theme	high	169:172	arg1	cost					174:177	high cost	169:177	high cost	169:177	Silicon/carbon composites have the disadvantages of large volume expansion and high cost, which limits their commercial application.
33155575	7	68	theme	sustainable	1196:1206	arg1	protection					1226:1235	the sustainable and environmental protection	1192:1235	the sustainable and environmental protection of lithium-ion battery anode materials	1192:1274	This method provides a reference for the sustainable and environmental protection of lithium-ion battery anode materials.
33155575	4	69	theme	%	590:590	arg1	content					600:606	20% silicon content	588:606	20% silicon content	588:606	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	0	70	theme	composite	79:87	arg1	treatment					18:26	Biological enzyme treatment	0:26	Biological enzyme treatment of starch-based lithium-ion battery silicon-carbon composite.	0:88	Biological enzyme treatment of starch-based lithium-ion battery silicon-carbon composite.
33155575	4	71	theme	specific	716:723	arg1	capacity					725:732	the specific capacity	712:732	the specific capacity	712:732	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
33155575	6	72	theme	derived	1070:1076	arg1	layer					1078:1082	the starch derived layer	1059:1082	the starch derived layer	1059:1082	Its excellent properties and stability are attributed to the abundant porosity of the carbon in the starch derived layer, which improves the structural stability and electrochemical kinetics.
33155575	1	73	theme	cost	174:177	arg1	disadvantages					125:137	the disadvantages	121:137	the disadvantages	121:137	Silicon/carbon composites have the disadvantages of large volume expansion and high cost, which limits their commercial application.
33155575	4	74	theme	20	588:589	arg1	%					590:590	%	590:590	%	590:590	The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g-1 and an initial Coulombic efficiency of 66% at 0.2 A g-1, and the specific capacity is maintained 450 mAh g-1 after 100 cycles.
32289410	0	0	theme	chitosan	92:99	arg1	films					125:129	brown rice starch-based chitosan biodegradable composite films	68:129	brown rice starch-based chitosan biodegradable composite films	68:129	Evaluation of the thermomechanical properties and biodegradation of brown rice starch-based chitosan biodegradable composite films.
32289410	3	1	with	structure	492:500	arg1	surface					470:476	a smooth surface	461:476	a smooth surface	461:476	Antimicrobial films with a smooth surface and a compact structure of brown rice starch were obtained.
32289410	4	2	theme	higher	564:569	arg1	proportion					571:580	a higher proportion	562:580	a higher proportion of chitosan in the polymer blends	562:614	The results showed that a higher proportion of chitosan in the polymer blends resulted in a substantial enhancement in the tensile strength (TS) and thermal stability of the film.
32289410	0	3	theme	starch-based	79:90	arg1	films					125:129	brown rice starch-based chitosan biodegradable composite films	68:129	brown rice starch-based chitosan biodegradable composite films	68:129	Evaluation of the thermomechanical properties and biodegradation of brown rice starch-based chitosan biodegradable composite films.
32289410	6	4	theme	reduced	1075:1081	arg1	uptake					1089:1094	a reduced water uptake	1073:1094	a reduced water uptake of the film	1073:1106	The addition of chitosan to the brown rice starch samples resulted in a reduced water uptake of the film.
32289410	2	5	theme	mechanical	340:349	arg1	properties					394:403	the mechanical, thermal, antimicrobial, and morphological properties	336:403	the mechanical, thermal, antimicrobial, and morphological properties of the films	336:416	In this study, the influence of the ratio of brown rice starch and chitosan on the mechanical, thermal, antimicrobial, and morphological properties of the films was investigated.
32289410	6	6	theme	water	1083:1087	arg1	uptake					1089:1094	a reduced water uptake	1073:1094	a reduced water uptake of the film	1073:1106	The addition of chitosan to the brown rice starch samples resulted in a reduced water uptake of the film.
32289410	0	7	theme	composite	115:123	arg1	films					125:129	brown rice starch-based chitosan biodegradable composite films	68:129	brown rice starch-based chitosan biodegradable composite films	68:129	Evaluation of the thermomechanical properties and biodegradation of brown rice starch-based chitosan biodegradable composite films.
32289410	2	8	theme	ratio	293:297	arg1	influence					276:284	the influence	272:284	the influence of the ratio of brown rice starch and chitosan on the mechanical, thermal, antimicrobial, and morphological properties of the films	272:416	In this study, the influence of the ratio of brown rice starch and chitosan on the mechanical, thermal, antimicrobial, and morphological properties of the films was investigated.
32289410	0	9	theme	biodegradable	101:113	arg1	films					125:129	brown rice starch-based chitosan biodegradable composite films	68:129	brown rice starch-based chitosan biodegradable composite films	68:129	Evaluation of the thermomechanical properties and biodegradation of brown rice starch-based chitosan biodegradable composite films.
32289410	5	10	from	values	874:879	arg1	elongation					849:858	the elongation	845:858	the elongation at break (EB) values of the BRS100, BRS30CH70, BRS50CH50, BRS70CH30, and CH100 samples	845:945	The TS values for BRS100, BRS30CH70, BRS50CH50, BRS70CH30, and CH100 were 3.7, 15.2, 10.2, 9.3, and 8.8 MPa, respectively, and the elongation at break (EB) values of the BRS100, BRS30CH70, BRS50CH50, BRS70CH30, and CH100 samples were 39.5%, 34.7%, 7.3%, 11.5%, and 6.9%, respectively.
32289410	5	10	from	values	874:879	arg1	%					956:956	39.5%	952:956	39.5%	952:956	The TS values for BRS100, BRS30CH70, BRS50CH50, BRS70CH30, and CH100 were 3.7, 15.2, 10.2, 9.3, and 8.8 MPa, respectively, and the elongation at break (EB) values of the BRS100, BRS30CH70, BRS50CH50, BRS70CH30, and CH100 samples were 39.5%, 34.7%, 7.3%, 11.5%, and 6.9%, respectively.
32289410	4	11	theme	thermal	687:693	arg1	stability					695:703	thermal stability	687:703	thermal stability	687:703	The results showed that a higher proportion of chitosan in the polymer blends resulted in a substantial enhancement in the tensile strength (TS) and thermal stability of the film.
32289410	3	12	theme	starch	516:521	arg1	structure					492:500	a compact structure	482:500	a compact structure of brown rice starch	482:521	Antimicrobial films with a smooth surface and a compact structure of brown rice starch were obtained.
32289410	3	12	theme	starch	516:521	arg1	films					450:454	Antimicrobial films	436:454	Antimicrobial films with a smooth surface	436:476	Antimicrobial films with a smooth surface and a compact structure of brown rice starch were obtained.
32289410	2	13	theme	morphological	380:392	arg1	properties					394:403	the mechanical, thermal, antimicrobial, and morphological properties	336:403	the mechanical, thermal, antimicrobial, and morphological properties of the films	336:416	In this study, the influence of the ratio of brown rice starch and chitosan on the mechanical, thermal, antimicrobial, and morphological properties of the films was investigated.
32289410	0	14	theme	films	125:129	arg1	biodegradation					50:63	biodegradation	50:63	biodegradation of brown rice starch-based chitosan biodegradable composite films	50:129	Evaluation of the thermomechanical properties and biodegradation of brown rice starch-based chitosan biodegradable composite films.
32289410	0	14	theme	films	125:129	arg1	properties					35:44	the thermomechanical properties	14:44	the thermomechanical properties	14:44	Evaluation of the thermomechanical properties and biodegradation of brown rice starch-based chitosan biodegradable composite films.
32289410	7	15	theme	water	1221:1225	arg1	absorption					1227:1236	its water absorption	1217:1236	its water absorption	1217:1236	The film with a balanced ratio of brown rice starch and chitosan exhibited excellent water resistance, with its water absorption being the lowest among all of the studied compositions.
32289410	7	15	theme	water	1221:1225	arg1	lowest					1248:1253	lowest	1248:1253	lowest	1248:1253	The film with a balanced ratio of brown rice starch and chitosan exhibited excellent water resistance, with its water absorption being the lowest among all of the studied compositions.
32289410	4	16	from	enhancement	642:652	arg1	TS					679:680	TS	679:680	TS	679:680	The results showed that a higher proportion of chitosan in the polymer blends resulted in a substantial enhancement in the tensile strength (TS) and thermal stability of the film.
32289410	4	16	from	enhancement	642:652	arg1	strength					669:676	tensile strength	661:676	tensile strength (TS)	661:681	The results showed that a higher proportion of chitosan in the polymer blends resulted in a substantial enhancement in the tensile strength (TS) and thermal stability of the film.
32289410	4	16	from	enhancement	642:652	arg1	stability					695:703	thermal stability	687:703	thermal stability	687:703	The results showed that a higher proportion of chitosan in the polymer blends resulted in a substantial enhancement in the tensile strength (TS) and thermal stability of the film.
32289410	1	17	theme	palm	199:202	arg1	oil					204:206	palm oil	199:206	palm oil	199:206	Biodegradable films composed of starch and chitosan plasticized by palm oil were fabricated via a solvent casting technique.
32289410	6	18	theme	starch	1046:1051	arg1	samples					1053:1059	the brown rice starch samples	1031:1059	the brown rice starch samples	1031:1059	The addition of chitosan to the brown rice starch samples resulted in a reduced water uptake of the film.
32289410	4	19	theme	substantial	630:640	arg1	enhancement					642:652	a substantial enhancement	628:652	a substantial enhancement in the tensile strength (TS) and thermal stability of the film	628:715	The results showed that a higher proportion of chitosan in the polymer blends resulted in a substantial enhancement in the tensile strength (TS) and thermal stability of the film.
32289410	2	20	theme	starch	313:318	arg1	ratio					293:297	the ratio	289:297	the ratio of brown rice starch and chitosan	289:331	In this study, the influence of the ratio of brown rice starch and chitosan on the mechanical, thermal, antimicrobial, and morphological properties of the films was investigated.
32289410	6	21	theme	rice	1041:1044	arg1	samples					1053:1059	the brown rice starch samples	1031:1059	the brown rice starch samples	1031:1059	The addition of chitosan to the brown rice starch samples resulted in a reduced water uptake of the film.
32289410	2	22	theme	rice	308:311	arg1	starch					313:318	brown rice starch	302:318	brown rice starch	302:318	In this study, the influence of the ratio of brown rice starch and chitosan on the mechanical, thermal, antimicrobial, and morphological properties of the films was investigated.
32289410	2	23	theme	antimicrobial	361:373	arg1	properties					394:403	the mechanical, thermal, antimicrobial, and morphological properties	336:403	the mechanical, thermal, antimicrobial, and morphological properties of the films	336:416	In this study, the influence of the ratio of brown rice starch and chitosan on the mechanical, thermal, antimicrobial, and morphological properties of the films was investigated.
32289410	0	24	theme	thermomechanical	18:33	arg1	properties					35:44	the thermomechanical properties	14:44	the thermomechanical properties	14:44	Evaluation of the thermomechanical properties and biodegradation of brown rice starch-based chitosan biodegradable composite films.
32289410	3	25	with	films	450:454	arg1	surface					470:476	a smooth surface	461:476	a smooth surface	461:476	Antimicrobial films with a smooth surface and a compact structure of brown rice starch were obtained.
32289410	7	26	theme	rice	1149:1152	arg1	starch					1154:1159	brown rice starch	1143:1159	brown rice starch	1143:1159	The film with a balanced ratio of brown rice starch and chitosan exhibited excellent water resistance, with its water absorption being the lowest among all of the studied compositions.
32289410	2	27	theme	brown	302:306	arg1	starch					313:318	brown rice starch	302:318	brown rice starch	302:318	In this study, the influence of the ratio of brown rice starch and chitosan on the mechanical, thermal, antimicrobial, and morphological properties of the films was investigated.
32289410	7	28	theme	starch	1154:1159	arg1	ratio					1134:1138	a balanced ratio	1123:1138	a balanced ratio of brown rice starch and chitosan	1123:1172	The film with a balanced ratio of brown rice starch and chitosan exhibited excellent water resistance, with its water absorption being the lowest among all of the studied compositions.
32289410	2	29	theme	films	412:416	arg1	properties					394:403	the mechanical, thermal, antimicrobial, and morphological properties	336:403	the mechanical, thermal, antimicrobial, and morphological properties of the films	336:416	In this study, the influence of the ratio of brown rice starch and chitosan on the mechanical, thermal, antimicrobial, and morphological properties of the films was investigated.
32289410	7	30	theme	water	1194:1198	arg1	resistance					1200:1209	excellent water resistance	1184:1209	excellent water resistance	1184:1209	The film with a balanced ratio of brown rice starch and chitosan exhibited excellent water resistance, with its water absorption being the lowest among all of the studied compositions.
32289410	4	31	dep	strength	669:676	arg1	the					657:659	the	657:659	the	657:659	The results showed that a higher proportion of chitosan in the polymer blends resulted in a substantial enhancement in the tensile strength (TS) and thermal stability of the film.
32289410	4	32	theme	film	712:715	arg1	TS					679:680	TS	679:680	TS	679:680	The results showed that a higher proportion of chitosan in the polymer blends resulted in a substantial enhancement in the tensile strength (TS) and thermal stability of the film.
32289410	4	32	theme	film	712:715	arg1	strength					669:676	tensile strength	661:676	tensile strength (TS)	661:681	The results showed that a higher proportion of chitosan in the polymer blends resulted in a substantial enhancement in the tensile strength (TS) and thermal stability of the film.
32289410	4	32	theme	film	712:715	arg1	stability					695:703	thermal stability	687:703	thermal stability	687:703	The results showed that a higher proportion of chitosan in the polymer blends resulted in a substantial enhancement in the tensile strength (TS) and thermal stability of the film.
32289410	7	33	theme	excellent	1184:1192	arg1	resistance					1200:1209	excellent water resistance	1184:1209	excellent water resistance	1184:1209	The film with a balanced ratio of brown rice starch and chitosan exhibited excellent water resistance, with its water absorption being the lowest among all of the studied compositions.
32289410	3	34	theme	smooth	463:468	arg1	surface					470:476	a smooth surface	461:476	a smooth surface	461:476	Antimicrobial films with a smooth surface and a compact structure of brown rice starch were obtained.
32289410	5	35	theme	samples	939:945	arg1	values					874:879	break (EB) values	863:879	break (EB) values of the BRS100, BRS30CH70, BRS50CH50, BRS70CH30, and CH100 samples	863:945	The TS values for BRS100, BRS30CH70, BRS50CH50, BRS70CH30, and CH100 were 3.7, 15.2, 10.2, 9.3, and 8.8 MPa, respectively, and the elongation at break (EB) values of the BRS100, BRS30CH70, BRS50CH50, BRS70CH30, and CH100 samples were 39.5%, 34.7%, 7.3%, 11.5%, and 6.9%, respectively.
32289410	6	36	theme	brown	1035:1039	arg1	samples					1053:1059	the brown rice starch samples	1031:1059	the brown rice starch samples	1031:1059	The addition of chitosan to the brown rice starch samples resulted in a reduced water uptake of the film.
32289410	0	37	theme	properties	35:44	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of the thermomechanical properties and biodegradation of brown rice starch-based chitosan biodegradable composite films.	0:130	Evaluation of the thermomechanical properties and biodegradation of brown rice starch-based chitosan biodegradable composite films.
32289410	7	38	theme	chitosan	1165:1172	arg1	ratio					1134:1138	a balanced ratio	1123:1138	a balanced ratio of brown rice starch and chitosan	1123:1172	The film with a balanced ratio of brown rice starch and chitosan exhibited excellent water resistance, with its water absorption being the lowest among all of the studied compositions.
32289410	1	39	theme	solvent	230:236	arg1	technique					246:254	a solvent casting technique	228:254	a solvent casting technique	228:254	Biodegradable films composed of starch and chitosan plasticized by palm oil were fabricated via a solvent casting technique.
32289410	7	40	theme	studied	1272:1278	arg1	compositions					1280:1291	the studied compositions	1268:1291	the studied compositions	1268:1291	The film with a balanced ratio of brown rice starch and chitosan exhibited excellent water resistance, with its water absorption being the lowest among all of the studied compositions.
32289410	6	41	theme	film	1103:1106	arg1	uptake					1089:1094	a reduced water uptake	1073:1094	a reduced water uptake of the film	1073:1106	The addition of chitosan to the brown rice starch samples resulted in a reduced water uptake of the film.
32289410	5	42	theme	TS	722:723	arg1	3.7					792:794	3.7	792:794	3.7	792:794	The TS values for BRS100, BRS30CH70, BRS50CH50, BRS70CH30, and CH100 were 3.7, 15.2, 10.2, 9.3, and 8.8 MPa, respectively, and the elongation at break (EB) values of the BRS100, BRS30CH70, BRS50CH50, BRS70CH30, and CH100 samples were 39.5%, 34.7%, 7.3%, 11.5%, and 6.9%, respectively.
32289410	5	42	theme	TS	722:723	arg1	values					725:730	The TS values	718:730	The TS values for BRS100, BRS30CH70, BRS50CH50, BRS70CH30, and CH100	718:785	The TS values for BRS100, BRS30CH70, BRS50CH50, BRS70CH30, and CH100 were 3.7, 15.2, 10.2, 9.3, and 8.8 MPa, respectively, and the elongation at break (EB) values of the BRS100, BRS30CH70, BRS50CH50, BRS70CH30, and CH100 samples were 39.5%, 34.7%, 7.3%, 11.5%, and 6.9%, respectively.
32289410	7	43	theme	brown	1143:1147	arg1	starch					1154:1159	brown rice starch	1143:1159	brown rice starch	1143:1159	The film with a balanced ratio of brown rice starch and chitosan exhibited excellent water resistance, with its water absorption being the lowest among all of the studied compositions.
32289410	1	44	theme	Biodegradable	132:144	arg1	films					146:150	Biodegradable films	132:150	Biodegradable films composed of starch and chitosan plasticized by palm oil	132:206	Biodegradable films composed of starch and chitosan plasticized by palm oil were fabricated via a solvent casting technique.
32289410	0	45	theme	biodegradation	50:63	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of the thermomechanical properties and biodegradation of brown rice starch-based chitosan biodegradable composite films.	0:130	Evaluation of the thermomechanical properties and biodegradation of brown rice starch-based chitosan biodegradable composite films.
32289410	6	46	theme	chitosan	1019:1026	arg1	addition					1007:1014	The addition	1003:1014	The addition of chitosan to the brown rice starch samples	1003:1059	The addition of chitosan to the brown rice starch samples resulted in a reduced water uptake of the film.
32289410	3	47	theme	compact	484:490	arg1	structure					492:500	a compact structure	482:500	a compact structure of brown rice starch	482:521	Antimicrobial films with a smooth surface and a compact structure of brown rice starch were obtained.
32289410	7	48	with	film	1113:1116	arg1	ratio					1134:1138	a balanced ratio	1123:1138	a balanced ratio of brown rice starch and chitosan	1123:1172	The film with a balanced ratio of brown rice starch and chitosan exhibited excellent water resistance, with its water absorption being the lowest among all of the studied compositions.
32289410	4	49	theme	tensile	661:667	arg1	TS					679:680	TS	679:680	TS	679:680	The results showed that a higher proportion of chitosan in the polymer blends resulted in a substantial enhancement in the tensile strength (TS) and thermal stability of the film.
32289410	4	49	theme	tensile	661:667	arg1	strength					669:676	tensile strength	661:676	tensile strength (TS)	661:681	The results showed that a higher proportion of chitosan in the polymer blends resulted in a substantial enhancement in the tensile strength (TS) and thermal stability of the film.
32289410	2	50	theme	chitosan	324:331	arg1	ratio					293:297	the ratio	289:297	the ratio of brown rice starch and chitosan	289:331	In this study, the influence of the ratio of brown rice starch and chitosan on the mechanical, thermal, antimicrobial, and morphological properties of the films was investigated.
32289410	2	51	from	influence	276:284	arg1	properties					394:403	the mechanical, thermal, antimicrobial, and morphological properties	336:403	the mechanical, thermal, antimicrobial, and morphological properties of the films	336:416	In this study, the influence of the ratio of brown rice starch and chitosan on the mechanical, thermal, antimicrobial, and morphological properties of the films was investigated.
32289410	1	52	theme	casting	238:244	arg1	technique					246:254	a solvent casting technique	228:254	a solvent casting technique	228:254	Biodegradable films composed of starch and chitosan plasticized by palm oil were fabricated via a solvent casting technique.
32289410	5	53	theme	break	863:867	arg1	values					874:879	break (EB) values	863:879	break (EB) values of the BRS100, BRS30CH70, BRS50CH50, BRS70CH30, and CH100 samples	863:945	The TS values for BRS100, BRS30CH70, BRS50CH50, BRS70CH30, and CH100 were 3.7, 15.2, 10.2, 9.3, and 8.8 MPa, respectively, and the elongation at break (EB) values of the BRS100, BRS30CH70, BRS50CH50, BRS70CH30, and CH100 samples were 39.5%, 34.7%, 7.3%, 11.5%, and 6.9%, respectively.
32289410	3	54	theme	rice	511:514	arg1	starch					516:521	brown rice starch	505:521	brown rice starch	505:521	Antimicrobial films with a smooth surface and a compact structure of brown rice starch were obtained.
32289410	4	55	theme	chitosan	585:592	arg1	proportion					571:580	a higher proportion	562:580	a higher proportion of chitosan in the polymer blends	562:614	The results showed that a higher proportion of chitosan in the polymer blends resulted in a substantial enhancement in the tensile strength (TS) and thermal stability of the film.
32289410	0	56	theme	rice	74:77	arg1	films					125:129	brown rice starch-based chitosan biodegradable composite films	68:129	brown rice starch-based chitosan biodegradable composite films	68:129	Evaluation of the thermomechanical properties and biodegradation of brown rice starch-based chitosan biodegradable composite films.
32289410	5	57	theme	EB	870:871	arg1	values					874:879	break (EB) values	863:879	break (EB) values of the BRS100, BRS30CH70, BRS50CH50, BRS70CH30, and CH100 samples	863:945	The TS values for BRS100, BRS30CH70, BRS50CH50, BRS70CH30, and CH100 were 3.7, 15.2, 10.2, 9.3, and 8.8 MPa, respectively, and the elongation at break (EB) values of the BRS100, BRS30CH70, BRS50CH50, BRS70CH30, and CH100 samples were 39.5%, 34.7%, 7.3%, 11.5%, and 6.9%, respectively.
32289410	3	58	theme	brown	505:509	arg1	starch					516:521	brown rice starch	505:521	brown rice starch	505:521	Antimicrobial films with a smooth surface and a compact structure of brown rice starch were obtained.
32289410	3	59	theme	Antimicrobial	436:448	arg1	films					450:454	Antimicrobial films	436:454	Antimicrobial films with a smooth surface	436:476	Antimicrobial films with a smooth surface and a compact structure of brown rice starch were obtained.
32289410	4	60	theme	polymer	601:607	arg1	blends					609:614	the polymer blends	597:614	the polymer blends	597:614	The results showed that a higher proportion of chitosan in the polymer blends resulted in a substantial enhancement in the tensile strength (TS) and thermal stability of the film.
32289410	0	61	theme	brown	68:72	arg1	films					125:129	brown rice starch-based chitosan biodegradable composite films	68:129	brown rice starch-based chitosan biodegradable composite films	68:129	Evaluation of the thermomechanical properties and biodegradation of brown rice starch-based chitosan biodegradable composite films.
32289410	7	62	theme	balanced	1125:1132	arg1	ratio					1134:1138	a balanced ratio	1123:1138	a balanced ratio of brown rice starch and chitosan	1123:1172	The film with a balanced ratio of brown rice starch and chitosan exhibited excellent water resistance, with its water absorption being the lowest among all of the studied compositions.
32289410	2	63	theme	thermal	352:358	arg1	properties					394:403	the mechanical, thermal, antimicrobial, and morphological properties	336:403	the mechanical, thermal, antimicrobial, and morphological properties of the films	336:416	In this study, the influence of the ratio of brown rice starch and chitosan on the mechanical, thermal, antimicrobial, and morphological properties of the films was investigated.
32289410	4	64	from	proportion	571:580	arg1	blends					609:614	the polymer blends	597:614	the polymer blends	597:614	The results showed that a higher proportion of chitosan in the polymer blends resulted in a substantial enhancement in the tensile strength (TS) and thermal stability of the film.
32573715	5	0	theme	different	1131:1139	arg1	vendors					1141:1147	different vendors	1131:1147	different vendors	1131:1147	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	6	1	from	content	1390:1396	arg1	cells					1440:1444	cells	1440:1444	cells	1440:1444	The high variability of different chondroitinases highlights the importance of testing enzyme activity and monitoring product formation in assessing the content and composition of chondroitin and DSs in cells and tissues.
32573715	6	1	from	content	1390:1396	arg1	tissues					1450:1456	tissues	1450:1456	tissues	1450:1456	The high variability of different chondroitinases highlights the importance of testing enzyme activity and monitoring product formation in assessing the content and composition of chondroitin and DSs in cells and tissues.
32573715	3	2	from	variation	512:520	arg1	specificity					542:552	specificity	542:552	specificity	542:552	While studying the structure of oncofetal CS, we noted a large variation in the activity and specificity of commercially available chondroitinases.
32573715	3	2	from	variation	512:520	arg1	activity					529:536	activity	529:536	activity	529:536	While studying the structure of oncofetal CS, we noted a large variation in the activity and specificity of commercially available chondroitinases.
32573715	5	3	theme	substrates	1198:1207	arg1	cleavage					1182:1189	cleavage	1182:1189	cleavage of the substrates and the generated products	1182:1234	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	6	4	theme	chondroitin	1417:1427	arg1	content					1390:1396	content	1390:1396	content	1390:1396	The high variability of different chondroitinases highlights the importance of testing enzyme activity and monitoring product formation in assessing the content and composition of chondroitin and DSs in cells and tissues.
32573715	6	4	theme	chondroitin	1417:1427	arg1	composition					1402:1412	composition	1402:1412	composition	1402:1412	The high variability of different chondroitinases highlights the importance of testing enzyme activity and monitoring product formation in assessing the content and composition of chondroitin and DSs in cells and tissues.
32573715	6	5	from	composition	1402:1412	arg1	cells					1440:1444	cells	1440:1444	cells	1440:1444	The high variability of different chondroitinases highlights the importance of testing enzyme activity and monitoring product formation in assessing the content and composition of chondroitin and DSs in cells and tissues.
32573715	6	5	from	composition	1402:1412	arg1	tissues					1450:1456	tissues	1450:1456	tissues	1450:1456	The high variability of different chondroitinases highlights the importance of testing enzyme activity and monitoring product formation in assessing the content and composition of chondroitin and DSs in cells and tissues.
32573715	4	6	from	vendors	906:912	arg1	ABC					877:879	ABC	877:879	ABC	877:879	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	4	6	from	vendors	906:912	arg1	B					889:889	B	889:889	B	889:889	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	4	6	from	vendors	906:912	arg1	chondroitinases					861:875	chondroitinases ABC, AC and B	861:889	chondroitinases ABC, AC and B from different vendors	861:912	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	4	6	from	vendors	906:912	arg1	AC					882:883	AC	882:883	AC	882:883	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	4	7	theme	bovine	809:814	arg1	A					819:819	commercially available bovine CS A	786:819	commercially available bovine CS A	786:819	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	5	8	theme	enzyme-specific	994:1008	arg1	activity					1010:1017	the enzyme-specific activity	990:1017	the enzyme-specific activity	990:1017	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	5	9	from	differences	1153:1163	arg1	activity					1098:1105	enzyme specific activity	1082:1105	enzyme specific activity of similar enzymes from different vendors	1082:1147	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	5	9	from	differences	1153:1163	arg1	extent					1172:1177	the extent	1168:1177	the extent of cleavage of the substrates and the generated products	1168:1234	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	4	10	theme	high-performance	647:662	arg1	spectrometry					691:702	high-performance liquid chromatography-mass spectrometry	647:702	high-performance liquid chromatography-mass spectrometry	647:702	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	1	11	theme	key	246:248	arg1	roles					250:254	key roles	246:254	key roles	246:254	Chondroitin sulfate (CS)and dermatan sulfate (DS) are negatively charged polysaccharides found abundantly in animal tissue and have been extensively described to play key roles in health and disease.
32573715	5	12	theme	large	970:974	arg1	variations					976:985	large variations	970:985	large variations in the enzyme-specific activity	970:1017	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	0	13	theme	Beware	0:5	arg1	chondroitinases					19:33	Beware, commercial chondroitinases	0:33	Beware, commercial chondroitinases	0:33	Beware, commercial chondroitinases vary in activity and substrate specificity.
32573715	5	14	theme	generated	1217:1225	arg1	products					1227:1234	the generated products	1213:1234	the generated products	1213:1234	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	5	15	from	variation	1069:1077	arg1	activity					1098:1105	enzyme specific activity	1082:1105	enzyme specific activity of similar enzymes from different vendors	1082:1147	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	5	15	from	variation	1069:1077	arg1	extent					1172:1177	the extent	1168:1177	the extent of cleavage of the substrates and the generated products	1168:1234	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	6	16	theme	different	1261:1269	arg1	chondroitinases					1271:1285	different chondroitinases	1261:1285	different chondroitinases	1261:1285	The high variability of different chondroitinases highlights the importance of testing enzyme activity and monitoring product formation in assessing the content and composition of chondroitin and DSs in cells and tissues.
32573715	6	17	theme	chondroitinases	1271:1285	arg1	variability					1246:1256	The high variability	1237:1256	The high variability of different chondroitinases	1237:1285	The high variability of different chondroitinases highlights the importance of testing enzyme activity and monitoring product formation in assessing the content and composition of chondroitin and DSs in cells and tissues.
32573715	2	18	dep	digestion	335:343	arg1	followed					396:403	followed	396:403	followed by chromatography and/or mass spectrometry	396:446	The most common method to analyze their structure is by digestion into disaccharides with bacterial chondroitinases, followed by chromatography and/or mass spectrometry.
32573715	1	19	theme	charged	144:150	arg1	sulfate					116:122	dermatan sulfate	107:122	dermatan sulfate (DS)	107:127	Chondroitin sulfate (CS)and dermatan sulfate (DS) are negatively charged polysaccharides found abundantly in animal tissue and have been extensively described to play key roles in health and disease.
32573715	1	19	theme	charged	144:150	arg1	polysaccharides					152:166	negatively charged polysaccharides	133:166	negatively charged polysaccharides found abundantly in animal tissue	133:200	Chondroitin sulfate (CS)and dermatan sulfate (DS) are negatively charged polysaccharides found abundantly in animal tissue and have been extensively described to play key roles in health and disease.
32573715	1	19	theme	charged	144:150	arg1	sulfate					91:97	Chondroitin sulfate	79:97	Chondroitin sulfate (CS)	79:102	Chondroitin sulfate (CS)and dermatan sulfate (DS) are negatively charged polysaccharides found abundantly in animal tissue and have been extensively described to play key roles in health and disease.
32573715	6	20	theme	DSs	1433:1435	arg1	content					1390:1396	content	1390:1396	content	1390:1396	The high variability of different chondroitinases highlights the importance of testing enzyme activity and monitoring product formation in assessing the content and composition of chondroitin and DSs in cells and tissues.
32573715	6	20	theme	DSs	1433:1435	arg1	composition					1402:1412	composition	1402:1412	composition	1402:1412	The high variability of different chondroitinases highlights the importance of testing enzyme activity and monitoring product formation in assessing the content and composition of chondroitin and DSs in cells and tissues.
32573715	5	21	theme	similar	1110:1116	arg1	enzymes					1118:1124	similar enzymes	1110:1124	similar enzymes from different vendors	1110:1147	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	4	22	theme	DS	845:846	arg1	digestion					773:781	the digestion	769:781	the digestion of commercially available bovine CS A, shark CS C and porcine DS	769:846	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	1	23	theme	Chondroitin	79:89	arg1	sulfate					116:122	dermatan sulfate	107:122	dermatan sulfate (DS)	107:127	Chondroitin sulfate (CS)and dermatan sulfate (DS) are negatively charged polysaccharides found abundantly in animal tissue and have been extensively described to play key roles in health and disease.
32573715	1	23	theme	Chondroitin	79:89	arg1	CS					100:101	CS	100:101	CS	100:101	Chondroitin sulfate (CS)and dermatan sulfate (DS) are negatively charged polysaccharides found abundantly in animal tissue and have been extensively described to play key roles in health and disease.
32573715	1	23	theme	Chondroitin	79:89	arg1	polysaccharides					152:166	negatively charged polysaccharides	133:166	negatively charged polysaccharides found abundantly in animal tissue	133:200	Chondroitin sulfate (CS)and dermatan sulfate (DS) are negatively charged polysaccharides found abundantly in animal tissue and have been extensively described to play key roles in health and disease.
32573715	1	23	theme	Chondroitin	79:89	arg1	sulfate					91:97	Chondroitin sulfate	79:97	Chondroitin sulfate (CS)	79:102	Chondroitin sulfate (CS)and dermatan sulfate (DS) are negatively charged polysaccharides found abundantly in animal tissue and have been extensively described to play key roles in health and disease.
32573715	5	24	theme	enzymes	1118:1124	arg1	activity					1098:1105	enzyme specific activity	1082:1105	enzyme specific activity of similar enzymes from different vendors	1082:1147	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	2	25	theme	common	288:293	arg1	method					295:300	The most common method	279:300	The most common method to analyze their structure	279:327	The most common method to analyze their structure is by digestion into disaccharides with bacterial chondroitinases, followed by chromatography and/or mass spectrometry.
32573715	4	26	theme	chromatography-mass	671:689	arg1	spectrometry					691:702	high-performance liquid chromatography-mass spectrometry	647:702	high-performance liquid chromatography-mass spectrometry	647:702	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	2	27	theme	mass	430:433	arg1	spectrometry					435:446	mass spectrometry	430:446	mass spectrometry	430:446	The most common method to analyze their structure is by digestion into disaccharides with bacterial chondroitinases, followed by chromatography and/or mass spectrometry.
32573715	2	28	dep	method	295:300	arg1	analyze					305:311	analyze	305:311	to analyze their structure	302:327	The most common method to analyze their structure is by digestion into disaccharides with bacterial chondroitinases, followed by chromatography and/or mass spectrometry.
32573715	4	29	theme	liquid	664:669	arg1	spectrometry					691:702	high-performance liquid chromatography-mass spectrometry	647:702	high-performance liquid chromatography-mass spectrometry	647:702	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	5	30	from	activity	1098:1105	arg1	vendors					1141:1147	different vendors	1131:1147	different vendors	1131:1147	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	5	31	theme	cleavage	1182:1189	arg1	extent					1172:1177	the extent	1168:1177	the extent of cleavage of the substrates and the generated products	1168:1234	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	5	32	theme	specific	1089:1096	arg1	activity					1098:1105	enzyme specific activity	1082:1105	enzyme specific activity of similar enzymes from different vendors	1082:1147	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	5	33	theme	assay	945:949	arg1	setup					951:955	a standardized assay setup	930:955	a standardized assay setup	930:955	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	4	34	theme	C	831:831	arg1	digestion					773:781	the digestion	769:781	the digestion of commercially available bovine CS A, shark CS C and porcine DS	769:846	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	5	35	theme	standardized	932:943	arg1	setup					951:955	a standardized assay setup	930:955	a standardized assay setup	930:955	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	3	36	theme	large	506:510	arg1	variation					512:520	a large variation	504:520	a large variation in the activity and specificity of commercially available chondroitinases	504:594	While studying the structure of oncofetal CS, we noted a large variation in the activity and specificity of commercially available chondroitinases.
32573715	5	37	theme	setup	951:955	arg1	Application					915:925	Application	915:925	Application of a standardized assay setup	915:955	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	1	38	theme	animal	188:193	arg1	tissue					195:200	animal tissue	188:200	animal tissue	188:200	Chondroitin sulfate (CS)and dermatan sulfate (DS) are negatively charged polysaccharides found abundantly in animal tissue and have been extensively described to play key roles in health and disease.
32573715	5	39	from	vendors	1141:1147	arg1	activity					1098:1105	enzyme specific activity	1082:1105	enzyme specific activity of similar enzymes from different vendors	1082:1147	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	5	39	from	vendors	1141:1147	arg1	enzymes					1118:1124	similar enzymes	1110:1124	similar enzymes from different vendors	1110:1147	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	3	40	theme	CS	491:492	arg1	structure					468:476	the structure	464:476	the structure of oncofetal CS	464:492	While studying the structure of oncofetal CS, we noted a large variation in the activity and specificity of commercially available chondroitinases.
32573715	6	41	theme	product	1355:1361	arg1	formation					1363:1371	product formation	1355:1371	product formation	1355:1371	The high variability of different chondroitinases highlights the importance of testing enzyme activity and monitoring product formation in assessing the content and composition of chondroitin and DSs in cells and tissues.
32573715	3	42	theme	chondroitinases	580:594	arg1	specificity					542:552	specificity	542:552	specificity	542:552	While studying the structure of oncofetal CS, we noted a large variation in the activity and specificity of commercially available chondroitinases.
32573715	3	42	theme	chondroitinases	580:594	arg1	activity					529:536	activity	529:536	activity	529:536	While studying the structure of oncofetal CS, we noted a large variation in the activity and specificity of commercially available chondroitinases.
32573715	6	43	theme	high	1241:1244	arg1	variability					1246:1256	The high variability	1237:1256	The high variability of different chondroitinases	1237:1285	The high variability of different chondroitinases highlights the importance of testing enzyme activity and monitoring product formation in assessing the content and composition of chondroitin and DSs in cells and tissues.
32573715	2	44	theme	bacterial	369:377	arg1	chondroitinases					379:393	bacterial chondroitinases	369:393	bacterial chondroitinases	369:393	The most common method to analyze their structure is by digestion into disaccharides with bacterial chondroitinases, followed by chromatography and/or mass spectrometry.
32573715	5	45	theme	enzyme	1082:1087	arg1	activity					1098:1105	enzyme specific activity	1082:1105	enzyme specific activity of similar enzymes from different vendors	1082:1147	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	4	46	theme	CS	828:829	arg1	C					831:831	shark CS C	822:831	shark CS C	822:831	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	4	47	theme	enzymes	630:636	arg1	kinetics					614:621	the kinetics	610:621	the kinetics of the enzymes	610:636	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	1	48	located	found	168:172	arg1	tissue					195:200	animal tissue	188:200	animal tissue	188:200	Chondroitin sulfate (CS)and dermatan sulfate (DS) are negatively charged polysaccharides found abundantly in animal tissue and have been extensively described to play key roles in health and disease.
32573715	1	48	located	found	168:172	arg2	sulfate					91:97	Chondroitin sulfate	79:97	Chondroitin sulfate (CS)	79:102	Chondroitin sulfate (CS)and dermatan sulfate (DS) are negatively charged polysaccharides found abundantly in animal tissue and have been extensively described to play key roles in health and disease.
32573715	1	48	located	found	168:172	arg2	polysaccharides					152:166	negatively charged polysaccharides	133:166	negatively charged polysaccharides found abundantly in animal tissue	133:200	Chondroitin sulfate (CS)and dermatan sulfate (DS) are negatively charged polysaccharides found abundantly in animal tissue and have been extensively described to play key roles in health and disease.
32573715	1	48	located	found	168:172	arg2	sulfate					116:122	dermatan sulfate	107:122	dermatan sulfate (DS)	107:127	Chondroitin sulfate (CS)and dermatan sulfate (DS) are negatively charged polysaccharides found abundantly in animal tissue and have been extensively described to play key roles in health and disease.
32573715	4	49	theme	porcine	837:843	arg1	DS					845:846	porcine DS	837:846	porcine DS	837:846	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	6	50	theme	enzyme	1324:1329	arg1	activity					1331:1338	enzyme activity	1324:1338	enzyme activity	1324:1338	The high variability of different chondroitinases highlights the importance of testing enzyme activity and monitoring product formation in assessing the content and composition of chondroitin and DSs in cells and tissues.
32573715	3	51	theme	oncofetal	481:489	arg1	CS					491:492	oncofetal CS	481:492	oncofetal CS	481:492	While studying the structure of oncofetal CS, we noted a large variation in the activity and specificity of commercially available chondroitinases.
32573715	4	52	dep	chondroitinases	861:875	arg1	ABC					877:879	ABC	877:879	ABC	877:879	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	4	52	dep	chondroitinases	861:875	arg1	B					889:889	B	889:889	B	889:889	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	4	52	dep	chondroitinases	861:875	arg1	chondroitinases					861:875	chondroitinases ABC, AC and B	861:889	chondroitinases ABC, AC and B from different vendors	861:912	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	4	52	dep	chondroitinases	861:875	arg1	AC					882:883	AC	882:883	AC	882:883	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	3	53	theme	available	570:578	arg1	chondroitinases					580:594	commercially available chondroitinases	557:594	commercially available chondroitinases	557:594	While studying the structure of oncofetal CS, we noted a large variation in the activity and specificity of commercially available chondroitinases.
32573715	1	54	theme	dermatan	107:114	arg1	sulfate					116:122	dermatan sulfate	107:122	dermatan sulfate (DS)	107:127	Chondroitin sulfate (CS)and dermatan sulfate (DS) are negatively charged polysaccharides found abundantly in animal tissue and have been extensively described to play key roles in health and disease.
32573715	1	54	theme	dermatan	107:114	arg1	sulfate					91:97	Chondroitin sulfate	79:97	Chondroitin sulfate (CS)	79:102	Chondroitin sulfate (CS)and dermatan sulfate (DS) are negatively charged polysaccharides found abundantly in animal tissue and have been extensively described to play key roles in health and disease.
32573715	1	54	theme	dermatan	107:114	arg1	polysaccharides					152:166	negatively charged polysaccharides	133:166	negatively charged polysaccharides found abundantly in animal tissue	133:200	Chondroitin sulfate (CS)and dermatan sulfate (DS) are negatively charged polysaccharides found abundantly in animal tissue and have been extensively described to play key roles in health and disease.
32573715	1	54	theme	dermatan	107:114	arg1	DS					125:126	DS	125:126	DS	125:126	Chondroitin sulfate (CS)and dermatan sulfate (DS) are negatively charged polysaccharides found abundantly in animal tissue and have been extensively described to play key roles in health and disease.
32573715	4	55	theme	different	896:904	arg1	vendors					906:912	different vendors	896:912	different vendors	896:912	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	4	56	theme	A	819:819	arg1	digestion					773:781	the digestion	769:781	the digestion of commercially available bovine CS A, shark CS C and porcine DS	769:846	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	5	57	theme	products	1227:1234	arg1	cleavage					1182:1189	cleavage	1182:1189	cleavage of the substrates and the generated products	1182:1234	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	4	58	theme	available	799:807	arg1	A					819:819	commercially available bovine CS A	786:819	commercially available bovine CS A	786:819	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	2	59	with	digestion	335:343	arg1	chondroitinases					379:393	bacterial chondroitinases	369:393	bacterial chondroitinases	369:393	The most common method to analyze their structure is by digestion into disaccharides with bacterial chondroitinases, followed by chromatography and/or mass spectrometry.
32573715	4	60	theme	CS	816:817	arg1	A					819:819	commercially available bovine CS A	786:819	commercially available bovine CS A	786:819	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	4	61	theme	oligosaccharide	729:743	arg1	products					745:752	the di- and oligosaccharide products	717:752	products	745:752	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	5	62	theme	large	1063:1067	arg1	variation					1069:1077	large variation	1063:1077	large variation in enzyme specific activity of similar enzymes from different vendors	1063:1147	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	4	63	theme	shark	822:826	arg1	CS					828:829	shark CS	822:829	shark CS C	822:831	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
32573715	3	64	dep	activity	529:536	arg1	the					525:527	the	525:527	the	525:527	While studying the structure of oncofetal CS, we noted a large variation in the activity and specificity of commercially available chondroitinases.
32573715	0	65	theme	substrate	56:64	arg1	specificity					66:76	substrate specificity	56:76	substrate specificity	56:76	Beware, commercial chondroitinases vary in activity and substrate specificity.
32573715	0	66	dep	Beware	0:5	arg1	commercial					8:17	commercial	8:17	commercial	8:17	Beware, commercial chondroitinases vary in activity and substrate specificity.
32573715	6	67	dep	content	1390:1396	arg1	the					1386:1388	the	1386:1388	the	1386:1388	The high variability of different chondroitinases highlights the importance of testing enzyme activity and monitoring product formation in assessing the content and composition of chondroitin and DSs in cells and tissues.
32573715	5	68	from	variations	976:985	arg1	activity					1010:1017	the enzyme-specific activity	990:1017	the enzyme-specific activity	990:1017	Application of a standardized assay setup demonstrated large variations in the enzyme-specific activity compared to the values provided by vendors, large variation in enzyme specific activity of similar enzymes from different vendors and differences in the extent of cleavage of the substrates and the generated products.
32573715	4	69	theme	di-	721:723	arg1	products					745:752	the di- and oligosaccharide products	717:752	products	745:752	Here studied the kinetics of the enzymes and used high-performance liquid chromatography-mass spectrometry to determine the di- and oligosaccharide products resulting from the digestion of commercially available bovine CS A, shark CS C and porcine DS, focusing on chondroitinases ABC, AC and B from different vendors.
33369080	3	0	theme	factor	357:362	arg1	liposomes					380:388	tissue factor (TF) integrated liposomes	350:388	tissue factor (TF) integrated liposomes	350:388	This work aims to develop an efficient hemostatic agent by incorporating tissue factor (TF) integrated liposomes and collagen, which are capable of augmenting different inherent hemostatic mechanisms, into hemostasis-stimulating alginate matrix.
33369080	5	1	theme	TCA	794:796	arg1	beads					798:802	the TCA beads	790:802	the TCA beads	790:802	When the TCA beads were introduced into simulated body fluid, a controlled release of the loaded TF-liposomes was observed, which also accelerated with the increase of temperature, obtaining intact free proteoliposomes as demonstrated by fluorescence measurement.
33369080	6	2	theme	blood	1123:1127	arg1	coagulation					1095:1105	the coagulation	1091:1105	the coagulation of whole rabbit blood	1091:1127	It is further seen that TCA beads induced the coagulation of whole rabbit blood in about 4.5 min, as compared to ~14.4 min for the control with only recalcified blood.
33369080	9	3	theme	potent	1576:1581	arg1	agents					1594:1599	potent hemostatic agents	1576:1599	potent hemostatic agents	1576:1599	Thus, the TCA hydrogel macrobeads may offer a potential platform for the development of potent hemostatic agents.
33369080	4	4	theme	alginate	557:564	arg1	collagen					544:551	collagen	544:551	collagen	544:551	The composite of TF, collagen and alginate (TCA) was made into hydrogel beads with a diameter range of 2.5-3.5 mm, followed by electron microscopy, infrared spectroscopy, rheological, and swelling characterization to confirm its composition and hydrogel nature.
33369080	4	4	theme	alginate	557:564	arg1	TF					540:541	TF	540:541	TF	540:541	The composite of TF, collagen and alginate (TCA) was made into hydrogel beads with a diameter range of 2.5-3.5 mm, followed by electron microscopy, infrared spectroscopy, rheological, and swelling characterization to confirm its composition and hydrogel nature.
33369080	4	4	theme	alginate	557:564	arg1	composite					527:535	The composite	523:535	The composite of TF, collagen and alginate (TCA)	523:570	The composite of TF, collagen and alginate (TCA) was made into hydrogel beads with a diameter range of 2.5-3.5 mm, followed by electron microscopy, infrared spectroscopy, rheological, and swelling characterization to confirm its composition and hydrogel nature.
33369080	4	4	theme	alginate	557:564	arg1	alginate					557:564	alginate	557:564	alginate (TCA)	557:570	The composite of TF, collagen and alginate (TCA) was made into hydrogel beads with a diameter range of 2.5-3.5 mm, followed by electron microscopy, infrared spectroscopy, rheological, and swelling characterization to confirm its composition and hydrogel nature.
33369080	7	5	from	alginate	1248:1255	arg1	beads					1264:1268	TCA beads	1260:1268	TCA beads	1260:1268	The lipidated TF, collagen and alginate in TCA beads showed a positive synergistic effect on coagulation, while among them a decreasing procoagulant effect was observed.
33369080	4	6	theme	swelling	711:718	arg1	characterization					720:735	swelling characterization	711:735	swelling characterization	711:735	The composite of TF, collagen and alginate (TCA) was made into hydrogel beads with a diameter range of 2.5-3.5 mm, followed by electron microscopy, infrared spectroscopy, rheological, and swelling characterization to confirm its composition and hydrogel nature.
33369080	8	7	theme	live/dead	1417:1425	arg1	assay					1432:1436	a live/dead cell assay	1415:1436	a live/dead cell assay	1415:1436	Finally, we demonstrated by a live/dead cell assay that TCA particles had undetectable cytotoxicity.
33369080	5	8	theme	free	983:986	arg1	proteoliposomes					988:1002	intact free proteoliposomes	976:1002	intact free proteoliposomes as demonstrated by fluorescence measurement	976:1046	When the TCA beads were introduced into simulated body fluid, a controlled release of the loaded TF-liposomes was observed, which also accelerated with the increase of temperature, obtaining intact free proteoliposomes as demonstrated by fluorescence measurement.
33369080	5	9	theme	intact	976:981	arg1	proteoliposomes					988:1002	intact free proteoliposomes	976:1002	intact free proteoliposomes as demonstrated by fluorescence measurement	976:1046	When the TCA beads were introduced into simulated body fluid, a controlled release of the loaded TF-liposomes was observed, which also accelerated with the increase of temperature, obtaining intact free proteoliposomes as demonstrated by fluorescence measurement.
33369080	5	10	theme	temperature	953:963	arg1	increase					941:948	the increase	937:948	the increase of temperature	937:963	When the TCA beads were introduced into simulated body fluid, a controlled release of the loaded TF-liposomes was observed, which also accelerated with the increase of temperature, obtaining intact free proteoliposomes as demonstrated by fluorescence measurement.
33369080	5	11	dep	proteoliposomes	988:1002	arg1	demonstrated					1007:1018	demonstrated	1007:1018	demonstrated by fluorescence measurement	1007:1046	When the TCA beads were introduced into simulated body fluid, a controlled release of the loaded TF-liposomes was observed, which also accelerated with the increase of temperature, obtaining intact free proteoliposomes as demonstrated by fluorescence measurement.
33369080	4	12	theme	diameter	608:615	arg1	range					617:621	a diameter range	606:621	a diameter range of 2.5-3.5 mm	606:635	The composite of TF, collagen and alginate (TCA) was made into hydrogel beads with a diameter range of 2.5-3.5 mm, followed by electron microscopy, infrared spectroscopy, rheological, and swelling characterization to confirm its composition and hydrogel nature.
33369080	3	13	theme	integrated	369:378	arg1	liposomes					380:388	tissue factor (TF) integrated liposomes	350:388	tissue factor (TF) integrated liposomes	350:388	This work aims to develop an efficient hemostatic agent by incorporating tissue factor (TF) integrated liposomes and collagen, which are capable of augmenting different inherent hemostatic mechanisms, into hemostasis-stimulating alginate matrix.
33369080	6	14	theme	whole	1110:1114	arg1	blood					1123:1127	whole rabbit blood	1110:1127	whole rabbit blood	1110:1127	It is further seen that TCA beads induced the coagulation of whole rabbit blood in about 4.5 min, as compared to ~14.4 min for the control with only recalcified blood.
33369080	1	15	theme	Uncontrolled	77:88	arg1	hemorrhage					90:99	Uncontrolled hemorrhage	77:99	Uncontrolled hemorrhage	77:99	Uncontrolled hemorrhage accounts for a significant proportion of annual mortality worldwide.
33369080	5	16	theme	loaded	875:880	arg1	TF-liposomes					882:893	the loaded TF-liposomes	871:893	the loaded TF-liposomes	871:893	When the TCA beads were introduced into simulated body fluid, a controlled release of the loaded TF-liposomes was observed, which also accelerated with the increase of temperature, obtaining intact free proteoliposomes as demonstrated by fluorescence measurement.
33369080	4	17	theme	TF	540:541	arg1	collagen					544:551	collagen	544:551	collagen	544:551	The composite of TF, collagen and alginate (TCA) was made into hydrogel beads with a diameter range of 2.5-3.5 mm, followed by electron microscopy, infrared spectroscopy, rheological, and swelling characterization to confirm its composition and hydrogel nature.
33369080	4	17	theme	TF	540:541	arg1	TF					540:541	TF	540:541	TF	540:541	The composite of TF, collagen and alginate (TCA) was made into hydrogel beads with a diameter range of 2.5-3.5 mm, followed by electron microscopy, infrared spectroscopy, rheological, and swelling characterization to confirm its composition and hydrogel nature.
33369080	4	17	theme	TF	540:541	arg1	composite					527:535	The composite	523:535	The composite of TF, collagen and alginate (TCA)	523:570	The composite of TF, collagen and alginate (TCA) was made into hydrogel beads with a diameter range of 2.5-3.5 mm, followed by electron microscopy, infrared spectroscopy, rheological, and swelling characterization to confirm its composition and hydrogel nature.
33369080	4	17	theme	TF	540:541	arg1	alginate					557:564	alginate	557:564	alginate (TCA)	557:570	The composite of TF, collagen and alginate (TCA) was made into hydrogel beads with a diameter range of 2.5-3.5 mm, followed by electron microscopy, infrared spectroscopy, rheological, and swelling characterization to confirm its composition and hydrogel nature.
33369080	9	18	theme	TCA	1498:1500	arg1	macrobeads					1511:1520	the TCA hydrogel macrobeads	1494:1520	the TCA hydrogel macrobeads	1494:1520	Thus, the TCA hydrogel macrobeads may offer a potential platform for the development of potent hemostatic agents.
33369080	6	19	theme	rabbit	1116:1121	arg1	blood					1123:1127	whole rabbit blood	1110:1127	whole rabbit blood	1110:1127	It is further seen that TCA beads induced the coagulation of whole rabbit blood in about 4.5 min, as compared to ~14.4 min for the control with only recalcified blood.
33369080	4	20	theme	infrared	671:678	arg1	spectroscopy					680:691	infrared spectroscopy	671:691	infrared spectroscopy	671:691	The composite of TF, collagen and alginate (TCA) was made into hydrogel beads with a diameter range of 2.5-3.5 mm, followed by electron microscopy, infrared spectroscopy, rheological, and swelling characterization to confirm its composition and hydrogel nature.
33369080	5	21	theme	body	835:838	arg1	fluid					840:844	simulated body fluid	825:844	simulated body fluid	825:844	When the TCA beads were introduced into simulated body fluid, a controlled release of the loaded TF-liposomes was observed, which also accelerated with the increase of temperature, obtaining intact free proteoliposomes as demonstrated by fluorescence measurement.
33369080	4	22	theme	hydrogel	768:775	arg1	nature					777:782	hydrogel nature	768:782	hydrogel nature	768:782	The composite of TF, collagen and alginate (TCA) was made into hydrogel beads with a diameter range of 2.5-3.5 mm, followed by electron microscopy, infrared spectroscopy, rheological, and swelling characterization to confirm its composition and hydrogel nature.
33369080	3	23	theme	hemostatic	455:464	arg1	mechanisms					466:475	different inherent hemostatic mechanisms	436:475	different inherent hemostatic mechanisms	436:475	This work aims to develop an efficient hemostatic agent by incorporating tissue factor (TF) integrated liposomes and collagen, which are capable of augmenting different inherent hemostatic mechanisms, into hemostasis-stimulating alginate matrix.
33369080	7	24	from	TF	1231:1232	arg1	beads					1264:1268	TCA beads	1260:1268	TCA beads	1260:1268	The lipidated TF, collagen and alginate in TCA beads showed a positive synergistic effect on coagulation, while among them a decreasing procoagulant effect was observed.
33369080	8	25	theme	TCA	1443:1445	arg1	particles					1447:1455	TCA particles	1443:1455	TCA particles	1443:1455	Finally, we demonstrated by a live/dead cell assay that TCA particles had undetectable cytotoxicity.
33369080	0	26	theme	hydrogel	39:46	arg1	agent					70:74	a hemostatic agent	57:74	a hemostatic agent	57:74	Tissue factor-loaded collagen/alginate hydrogel beads as a hemostatic agent.
33369080	0	26	theme	hydrogel	39:46	arg1	beads					48:52	collagen/alginate hydrogel beads	21:52	collagen/alginate hydrogel beads	21:52	Tissue factor-loaded collagen/alginate hydrogel beads as a hemostatic agent.
33369080	6	27	with	control	1180:1186	arg1	blood					1210:1214	only recalcified blood	1193:1214	only recalcified blood	1193:1214	It is further seen that TCA beads induced the coagulation of whole rabbit blood in about 4.5 min, as compared to ~14.4 min for the control with only recalcified blood.
33369080	3	28	theme	efficient	306:314	arg1	agent					327:331	an efficient hemostatic agent	303:331	an efficient hemostatic agent	303:331	This work aims to develop an efficient hemostatic agent by incorporating tissue factor (TF) integrated liposomes and collagen, which are capable of augmenting different inherent hemostatic mechanisms, into hemostasis-stimulating alginate matrix.
33369080	6	29	theme	TCA	1073:1075	arg1	beads					1077:1081	TCA beads	1073:1081	TCA beads	1073:1081	It is further seen that TCA beads induced the coagulation of whole rabbit blood in about 4.5 min, as compared to ~14.4 min for the control with only recalcified blood.
33369080	4	30	theme	electron	650:657	arg1	microscopy					659:668	electron microscopy	650:668	electron microscopy	650:668	The composite of TF, collagen and alginate (TCA) was made into hydrogel beads with a diameter range of 2.5-3.5 mm, followed by electron microscopy, infrared spectroscopy, rheological, and swelling characterization to confirm its composition and hydrogel nature.
33369080	0	31	theme	collagen/alginate	21:37	arg1	agent					70:74	a hemostatic agent	57:74	a hemostatic agent	57:74	Tissue factor-loaded collagen/alginate hydrogel beads as a hemostatic agent.
33369080	0	31	theme	collagen/alginate	21:37	arg1	beads					48:52	collagen/alginate hydrogel beads	21:52	collagen/alginate hydrogel beads	21:52	Tissue factor-loaded collagen/alginate hydrogel beads as a hemostatic agent.
33369080	6	32	theme	recalcified	1198:1208	arg1	blood					1210:1214	only recalcified blood	1193:1214	only recalcified blood	1193:1214	It is further seen that TCA beads induced the coagulation of whole rabbit blood in about 4.5 min, as compared to ~14.4 min for the control with only recalcified blood.
33369080	3	33	theme	hemostatic	316:325	arg1	agent					327:331	an efficient hemostatic agent	303:331	an efficient hemostatic agent	303:331	This work aims to develop an efficient hemostatic agent by incorporating tissue factor (TF) integrated liposomes and collagen, which are capable of augmenting different inherent hemostatic mechanisms, into hemostasis-stimulating alginate matrix.
33369080	2	34	theme	composites	212:221	arg1	development					174:184	The development	170:184	The development of bioinspired hemostatic composites	170:221	The development of bioinspired hemostatic composites can effectively reduce hemorrhage and related deaths.
33369080	1	35	theme	significant	116:126	arg1	proportion					128:137	a significant proportion	114:137	a significant proportion of annual mortality worldwide	114:167	Uncontrolled hemorrhage accounts for a significant proportion of annual mortality worldwide.
33369080	9	36	theme	potential	1534:1542	arg1	platform					1544:1551	a potential platform	1532:1551	a potential platform for the development of potent hemostatic agents	1532:1599	Thus, the TCA hydrogel macrobeads may offer a potential platform for the development of potent hemostatic agents.
33369080	7	37	theme	TCA	1260:1262	arg1	beads					1264:1268	TCA beads	1260:1268	TCA beads	1260:1268	The lipidated TF, collagen and alginate in TCA beads showed a positive synergistic effect on coagulation, while among them a decreasing procoagulant effect was observed.
33369080	3	38	theme	different	436:444	arg1	mechanisms					466:475	different inherent hemostatic mechanisms	436:475	different inherent hemostatic mechanisms	436:475	This work aims to develop an efficient hemostatic agent by incorporating tissue factor (TF) integrated liposomes and collagen, which are capable of augmenting different inherent hemostatic mechanisms, into hemostasis-stimulating alginate matrix.
33369080	4	39	theme	hydrogel	586:593	arg1	beads					595:599	hydrogel beads	586:599	hydrogel beads	586:599	The composite of TF, collagen and alginate (TCA) was made into hydrogel beads with a diameter range of 2.5-3.5 mm, followed by electron microscopy, infrared spectroscopy, rheological, and swelling characterization to confirm its composition and hydrogel nature.
33369080	2	40	theme	hemostatic	201:210	arg1	composites					212:221	bioinspired hemostatic composites	189:221	bioinspired hemostatic composites	189:221	The development of bioinspired hemostatic composites can effectively reduce hemorrhage and related deaths.
33369080	3	41	theme	hemostasis-stimulating	483:504	arg1	matrix					515:520	hemostasis-stimulating alginate matrix	483:520	hemostasis-stimulating alginate matrix	483:520	This work aims to develop an efficient hemostatic agent by incorporating tissue factor (TF) integrated liposomes and collagen, which are capable of augmenting different inherent hemostatic mechanisms, into hemostasis-stimulating alginate matrix.
33369080	3	42	theme	inherent	446:453	arg1	mechanisms					466:475	different inherent hemostatic mechanisms	436:475	different inherent hemostatic mechanisms	436:475	This work aims to develop an efficient hemostatic agent by incorporating tissue factor (TF) integrated liposomes and collagen, which are capable of augmenting different inherent hemostatic mechanisms, into hemostasis-stimulating alginate matrix.
33369080	2	43	theme	bioinspired	189:199	arg1	composites					212:221	bioinspired hemostatic composites	189:221	bioinspired hemostatic composites	189:221	The development of bioinspired hemostatic composites can effectively reduce hemorrhage and related deaths.
33369080	7	44	from	effect	1300:1305	arg1	coagulation					1310:1320	coagulation	1310:1320	coagulation	1310:1320	The lipidated TF, collagen and alginate in TCA beads showed a positive synergistic effect on coagulation, while among them a decreasing procoagulant effect was observed.
33369080	3	45	theme	alginate	506:513	arg1	matrix					515:520	hemostasis-stimulating alginate matrix	483:520	hemostasis-stimulating alginate matrix	483:520	This work aims to develop an efficient hemostatic agent by incorporating tissue factor (TF) integrated liposomes and collagen, which are capable of augmenting different inherent hemostatic mechanisms, into hemostasis-stimulating alginate matrix.
33369080	0	46	theme	hemostatic	59:68	arg1	agent					70:74	a hemostatic agent	57:74	a hemostatic agent	57:74	Tissue factor-loaded collagen/alginate hydrogel beads as a hemostatic agent.
33369080	0	46	theme	hemostatic	59:68	arg1	beads					48:52	collagen/alginate hydrogel beads	21:52	collagen/alginate hydrogel beads	21:52	Tissue factor-loaded collagen/alginate hydrogel beads as a hemostatic agent.
33369080	2	47	theme	related	261:267	arg1	deaths					269:274	hemorrhage and related deaths	246:274	hemorrhage and related deaths	246:274	The development of bioinspired hemostatic composites can effectively reduce hemorrhage and related deaths.
33369080	7	48	theme	positive	1279:1286	arg1	effect					1300:1305	a positive synergistic effect	1277:1305	a positive synergistic effect on coagulation	1277:1320	The lipidated TF, collagen and alginate in TCA beads showed a positive synergistic effect on coagulation, while among them a decreasing procoagulant effect was observed.
33369080	5	49	theme	simulated	825:833	arg1	fluid					840:844	simulated body fluid	825:844	simulated body fluid	825:844	When the TCA beads were introduced into simulated body fluid, a controlled release of the loaded TF-liposomes was observed, which also accelerated with the increase of temperature, obtaining intact free proteoliposomes as demonstrated by fluorescence measurement.
33369080	3	50	theme	tissue	350:355	arg1	TF					365:366	TF	365:366	TF	365:366	This work aims to develop an efficient hemostatic agent by incorporating tissue factor (TF) integrated liposomes and collagen, which are capable of augmenting different inherent hemostatic mechanisms, into hemostasis-stimulating alginate matrix.
33369080	3	50	theme	tissue	350:355	arg1	factor					357:362	tissue factor	350:362	tissue factor (TF) integrated liposomes	350:388	This work aims to develop an efficient hemostatic agent by incorporating tissue factor (TF) integrated liposomes and collagen, which are capable of augmenting different inherent hemostatic mechanisms, into hemostasis-stimulating alginate matrix.
33369080	2	51	theme	hemorrhage	246:255	arg1	deaths					269:274	hemorrhage and related deaths	246:274	hemorrhage and related deaths	246:274	The development of bioinspired hemostatic composites can effectively reduce hemorrhage and related deaths.
33369080	7	52	theme	synergistic	1288:1298	arg1	effect					1300:1305	a positive synergistic effect	1277:1305	a positive synergistic effect on coagulation	1277:1320	The lipidated TF, collagen and alginate in TCA beads showed a positive synergistic effect on coagulation, while among them a decreasing procoagulant effect was observed.
33369080	8	53	theme	cell	1427:1430	arg1	assay					1432:1436	a live/dead cell assay	1415:1436	a live/dead cell assay	1415:1436	Finally, we demonstrated by a live/dead cell assay that TCA particles had undetectable cytotoxicity.
33369080	1	54	theme	annual	142:147	arg1	worldwide					159:167	annual mortality worldwide	142:167	annual mortality worldwide	142:167	Uncontrolled hemorrhage accounts for a significant proportion of annual mortality worldwide.
33369080	9	55	theme	hemostatic	1583:1592	arg1	agents					1594:1599	potent hemostatic agents	1576:1599	potent hemostatic agents	1576:1599	Thus, the TCA hydrogel macrobeads may offer a potential platform for the development of potent hemostatic agents.
33369080	4	56	theme	2.5-3.5 mm	626:635	arg1	range					617:621	a diameter range	606:621	a diameter range of 2.5-3.5 mm	606:635	The composite of TF, collagen and alginate (TCA) was made into hydrogel beads with a diameter range of 2.5-3.5 mm, followed by electron microscopy, infrared spectroscopy, rheological, and swelling characterization to confirm its composition and hydrogel nature.
33369080	7	57	from	collagen	1235:1242	arg1	beads					1264:1268	TCA beads	1260:1268	TCA beads	1260:1268	The lipidated TF, collagen and alginate in TCA beads showed a positive synergistic effect on coagulation, while among them a decreasing procoagulant effect was observed.
33369080	5	58	theme	fluorescence	1023:1034	arg1	measurement					1036:1046	fluorescence measurement	1023:1046	fluorescence measurement	1023:1046	When the TCA beads were introduced into simulated body fluid, a controlled release of the loaded TF-liposomes was observed, which also accelerated with the increase of temperature, obtaining intact free proteoliposomes as demonstrated by fluorescence measurement.
33369080	1	59	theme	mortality	149:157	arg1	worldwide					159:167	annual mortality worldwide	142:167	annual mortality worldwide	142:167	Uncontrolled hemorrhage accounts for a significant proportion of annual mortality worldwide.
33369080	9	60	theme	hydrogel	1502:1509	arg1	macrobeads					1511:1520	the TCA hydrogel macrobeads	1494:1520	the TCA hydrogel macrobeads	1494:1520	Thus, the TCA hydrogel macrobeads may offer a potential platform for the development of potent hemostatic agents.
33369080	8	61	theme	undetectable	1461:1472	arg1	cytotoxicity					1474:1485	undetectable cytotoxicity	1461:1485	undetectable cytotoxicity	1461:1485	Finally, we demonstrated by a live/dead cell assay that TCA particles had undetectable cytotoxicity.
33369080	7	62	theme	decreasing	1342:1351	arg1	effect					1366:1371	a decreasing procoagulant effect	1340:1371	a decreasing procoagulant effect	1340:1371	The lipidated TF, collagen and alginate in TCA beads showed a positive synergistic effect on coagulation, while among them a decreasing procoagulant effect was observed.
33369080	5	63	theme	controlled	849:858	arg1	release					860:866	a controlled release	847:866	a controlled release of the loaded TF-liposomes	847:893	When the TCA beads were introduced into simulated body fluid, a controlled release of the loaded TF-liposomes was observed, which also accelerated with the increase of temperature, obtaining intact free proteoliposomes as demonstrated by fluorescence measurement.
33369080	1	64	theme	worldwide	159:167	arg1	proportion					128:137	a significant proportion	114:137	a significant proportion of annual mortality worldwide	114:167	Uncontrolled hemorrhage accounts for a significant proportion of annual mortality worldwide.
33369080	5	65	theme	TF-liposomes	882:893	arg1	release					860:866	a controlled release	847:866	a controlled release of the loaded TF-liposomes	847:893	When the TCA beads were introduced into simulated body fluid, a controlled release of the loaded TF-liposomes was observed, which also accelerated with the increase of temperature, obtaining intact free proteoliposomes as demonstrated by fluorescence measurement.
33369080	9	66	theme	agents	1594:1599	arg1	development					1561:1571	the development	1557:1571	the development of potent hemostatic agents	1557:1599	Thus, the TCA hydrogel macrobeads may offer a potential platform for the development of potent hemostatic agents.
33369080	4	67	theme	collagen	544:551	arg1	collagen					544:551	collagen	544:551	collagen	544:551	The composite of TF, collagen and alginate (TCA) was made into hydrogel beads with a diameter range of 2.5-3.5 mm, followed by electron microscopy, infrared spectroscopy, rheological, and swelling characterization to confirm its composition and hydrogel nature.
33369080	4	67	theme	collagen	544:551	arg1	TF					540:541	TF	540:541	TF	540:541	The composite of TF, collagen and alginate (TCA) was made into hydrogel beads with a diameter range of 2.5-3.5 mm, followed by electron microscopy, infrared spectroscopy, rheological, and swelling characterization to confirm its composition and hydrogel nature.
33369080	4	67	theme	collagen	544:551	arg1	composite					527:535	The composite	523:535	The composite of TF, collagen and alginate (TCA)	523:570	The composite of TF, collagen and alginate (TCA) was made into hydrogel beads with a diameter range of 2.5-3.5 mm, followed by electron microscopy, infrared spectroscopy, rheological, and swelling characterization to confirm its composition and hydrogel nature.
33369080	4	67	theme	collagen	544:551	arg1	alginate					557:564	alginate	557:564	alginate (TCA)	557:570	The composite of TF, collagen and alginate (TCA) was made into hydrogel beads with a diameter range of 2.5-3.5 mm, followed by electron microscopy, infrared spectroscopy, rheological, and swelling characterization to confirm its composition and hydrogel nature.
33369080	7	68	theme	procoagulant	1353:1364	arg1	effect					1366:1371	a decreasing procoagulant effect	1340:1371	a decreasing procoagulant effect	1340:1371	The lipidated TF, collagen and alginate in TCA beads showed a positive synergistic effect on coagulation, while among them a decreasing procoagulant effect was observed.
33369080	8	69	contain	had	1457:1459	arg1	particles					1447:1455	TCA particles	1443:1455	TCA particles	1443:1455	Finally, we demonstrated by a live/dead cell assay that TCA particles had undetectable cytotoxicity.
33369080	8	69	contain	had	1457:1459	arg2	cytotoxicity					1474:1485	undetectable cytotoxicity	1461:1485	undetectable cytotoxicity	1461:1485	Finally, we demonstrated by a live/dead cell assay that TCA particles had undetectable cytotoxicity.
33369080	7	70	theme	lipidated	1221:1229	arg1	TF					1231:1232	The lipidated TF	1217:1232	The lipidated TF	1217:1232	The lipidated TF, collagen and alginate in TCA beads showed a positive synergistic effect on coagulation, while among them a decreasing procoagulant effect was observed.
33513982	1	0	from	microalgae	172:181	arg1	EPS					155:157	EPS	155:157	EPS	155:157	Exopolysaccharide (EPS) from marine microalgae are promising sources of a new generation of drugs.
33513982	1	0	from	microalgae	172:181	arg1	Exopolysaccharide					136:152	Exopolysaccharide	136:152	Exopolysaccharide (EPS) from marine microalgae	136:181	Exopolysaccharide (EPS) from marine microalgae are promising sources of a new generation of drugs.
33513982	1	0	from	microalgae	172:181	arg1	sources					197:203	promising sources	187:203	promising sources of a new generation of drugs	187:232	Exopolysaccharide (EPS) from marine microalgae are promising sources of a new generation of drugs.
33513982	5	1	theme	native	736:741	arg1	EPS					743:745	the native EPS	732:745	the native EPS	732:745	For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain.
33513982	3	2	theme	anti-fungal	475:485	arg1	activities					503:512	anti-fungal and antibiofilm activities	475:512	activities	503:512	In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells.
33513982	3	2	theme	anti-fungal	475:485	arg1	activities					442:451	different biological activities	421:451	different biological activities	421:451	In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells.
33513982	8	3	theme	polymers	1327:1334	arg1	properties					1313:1322	the intrinsic properties	1299:1322	the intrinsic properties of polymers	1299:1334	Finally, analyses of polymers composition and viscosity measurements were conducted on all samples, in order to propose hypotheses involving the activities caused by the intrinsic properties of polymers.
33513982	7	4	theme	masses	1041:1046	arg1	fractions					1048:1056	lower molar masses fractions	1029:1056	lower molar masses fractions	1029:1056	Concerning breast cancer cells, lower molar masses fractions appeared to be more efficient, with a reduction of viability of up to 55%.
33513982	3	5	theme	Candida	517:523	arg1	albicans					525:532	Candida albicans	517:532	Candida albicans	517:532	In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells.
33513982	0	6	theme	Biofilm	98:104	arg1	Formation					106:114	Biofilm Formation	98:114	Biofilm Formation	98:114	Potential of Exopolysaccharide from Porphyridium marinum to Contend with Bacterial Proliferation, Biofilm Formation, and Breast Cancer.
33513982	7	7	theme	viability	1109:1117	arg1	reduction					1096:1104	a reduction	1094:1104	a reduction of viability of up to 55%	1094:1130	Concerning breast cancer cells, lower molar masses fractions appeared to be more efficient, with a reduction of viability of up to 55%.
33513982	7	8	theme	lower	1029:1033	arg1	masses					1041:1046	lower molar masses	1029:1046	lower molar masses fractions	1029:1056	Concerning breast cancer cells, lower molar masses fractions appeared to be more efficient, with a reduction of viability of up to 55%.
33513982	5	9	from	µg/mL	845:849	arg1	efficiency					766:775	a better efficiency	757:775	a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL	757:849	For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain.
33513982	1	10	theme	marine	165:170	arg1	microalgae					172:181	marine microalgae	165:181	marine microalgae	165:181	Exopolysaccharide (EPS) from marine microalgae are promising sources of a new generation of drugs.
33513982	5	11	theme	antibiofilm	708:718	arg1	activities					720:729	antibacterial and antibiofilm activities	690:729	activities	720:729	For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain.
33513982	0	12	from	marinum	49:55	arg1	Potential					0:8	Potential	0:8	Potential of Exopolysaccharide from Porphyridium marinum	0:55	Potential of Exopolysaccharide from Porphyridium marinum to Contend with Bacterial Proliferation, Biofilm Formation, and Breast Cancer.
33513982	7	13	theme	molar	1035:1039	arg1	masses					1041:1046	lower molar masses	1029:1046	lower molar masses fractions	1029:1056	Concerning breast cancer cells, lower molar masses fractions appeared to be more efficient, with a reduction of viability of up to 55%.
33513982	7	14	theme	cancer	1015:1020	arg1	cells					1022:1026	breast cancer cells	1008:1026	breast cancer cells	1008:1026	Concerning breast cancer cells, lower molar masses fractions appeared to be more efficient, with a reduction of viability of up to 55%.
33513982	2	15	theme	them	251:254	arg1	them					251:254	them	251:254	them	251:254	However, lot of them remain to be discovered and tested.
33513982	2	15	theme	them	251:254	arg1	lot					244:246	lot	244:246	lot of them	244:254	However, lot of them remain to be discovered and tested.
33513982	5	16	theme	62.5	826:829	arg1	µg/mL					831:835	µg/mL	831:835	µg/mL	831:835	For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain.
33513982	7	17	theme	%	1130:1130	arg1	viability					1109:1117	viability	1109:1117	viability of up to 55%	1109:1130	Concerning breast cancer cells, lower molar masses fractions appeared to be more efficient, with a reduction of viability of up to 55%.
33513982	8	18	theme	polymers	1154:1161	arg1	composition					1163:1173	polymers composition	1154:1173	polymers composition	1154:1173	Finally, analyses of polymers composition and viscosity measurements were conducted on all samples, in order to propose hypotheses involving the activities caused by the intrinsic properties of polymers.
33513982	8	19	theme	composition	1163:1173	arg1	analyses					1142:1149	analyses	1142:1149	analyses of polymers composition and viscosity measurements	1142:1200	Finally, analyses of polymers composition and viscosity measurements were conducted on all samples, in order to propose hypotheses involving the activities caused by the intrinsic properties of polymers.
33513982	1	20	theme	promising	187:195	arg1	Exopolysaccharide					136:152	Exopolysaccharide	136:152	Exopolysaccharide (EPS) from marine microalgae	136:181	Exopolysaccharide (EPS) from marine microalgae are promising sources of a new generation of drugs.
33513982	1	20	theme	promising	187:195	arg1	sources					197:203	promising sources	187:203	promising sources of a new generation of drugs	187:232	Exopolysaccharide (EPS) from marine microalgae are promising sources of a new generation of drugs.
33513982	5	21	theme	Minimum	782:788	arg1	MIC					816:818	MIC	816:818	MIC	816:818	For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain.
33513982	5	21	theme	Minimum	782:788	arg1	Concentration					801:813	Minimum Inhibitory Concentration	782:813	Minimum Inhibitory Concentration (MIC)	782:819	For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain.
33513982	7	22	theme	breast	1008:1013	arg1	cells					1022:1026	breast cancer cells	1008:1026	breast cancer cells	1008:1026	Concerning breast cancer cells, lower molar masses fractions appeared to be more efficient, with a reduction of viability of up to 55%.
33513982	5	23	with	efficiency	766:775	arg1	MIC					816:818	MIC	816:818	MIC	816:818	For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain.
33513982	5	23	with	efficiency	766:775	arg1	Concentration					801:813	Minimum Inhibitory Concentration	782:813	Minimum Inhibitory Concentration (MIC)	782:819	For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain.
33513982	8	24	theme	intrinsic	1303:1311	arg1	properties					1313:1322	the intrinsic properties	1299:1322	the intrinsic properties of polymers	1299:1334	Finally, analyses of polymers composition and viscosity measurements were conducted on all samples, in order to propose hypotheses involving the activities caused by the intrinsic properties of polymers.
33513982	3	25	theme	biological	431:440	arg1	activities					503:512	anti-fungal and antibiofilm activities	475:512	activities	503:512	In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells.
33513982	3	25	theme	biological	431:440	arg1	antibacterial					460:472	antibacterial	460:472	antibacterial	460:472	In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells.
33513982	3	25	theme	biological	431:440	arg1	activities					442:451	different biological activities	421:451	different biological activities	421:451	In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells.
33513982	0	26	theme	Exopolysaccharide	13:29	arg1	Potential					0:8	Potential	0:8	Potential of Exopolysaccharide from Porphyridium marinum	0:55	Potential of Exopolysaccharide from Porphyridium marinum to Contend with Bacterial Proliferation, Biofilm Formation, and Breast Cancer.
33513982	6	27	theme	biofilm	912:918	arg1	formation					920:928	the biofilm formation	908:928	the biofilm formation	908:928	For Candida albicans, the biofilm formation was reduced by about 90% by using only a 31.3 µg/mL concentration.
33513982	0	28	theme	Breast	121:126	arg1	Cancer					128:133	Breast Cancer	121:133	Breast Cancer	121:133	Potential of Exopolysaccharide from Porphyridium marinum to Contend with Bacterial Proliferation, Biofilm Formation, and Breast Cancer.
33513982	3	29	theme	cells	605:609	arg1	viability					571:579	the viability	567:579	the viability of murine breast cancer cells	567:609	In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells.
33513982	3	30	from	effects	556:562	arg1	viability					571:579	the viability	567:579	the viability of murine breast cancer cells	567:609	In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells.
33513982	5	31	theme	1000	840:843	arg1	µg/mL					831:835	µg/mL	831:835	µg/mL	831:835	For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain.
33513982	3	32	theme	High	374:377	arg1	Homogenizer					388:398	High Pressure Homogenizer	374:398	High Pressure Homogenizer	374:398	In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells.
33513982	5	33	dep	µg/mL	831:835	arg1	to					837:838	to	837:838	to	837:838	For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain.
33513982	8	34	theme	measurements	1189:1200	arg1	analyses					1142:1149	analyses	1142:1149	analyses of polymers composition and viscosity measurements	1142:1200	Finally, analyses of polymers composition and viscosity measurements were conducted on all samples, in order to propose hypotheses involving the activities caused by the intrinsic properties of polymers.
33513982	3	35	theme	Pressure	379:386	arg1	Homogenizer					388:398	High Pressure Homogenizer	374:398	High Pressure Homogenizer	374:398	In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells.
33513982	3	36	theme	breast	591:596	arg1	cells					605:609	murine breast cancer cells	584:609	murine breast cancer cells	584:609	In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells.
33513982	6	37	theme	µg/mL	976:980	arg1	concentration					982:994	only a 31.3 µg/mL concentration	964:994	only a 31.3 µg/mL concentration	964:994	For Candida albicans, the biofilm formation was reduced by about 90% by using only a 31.3 µg/mL concentration.
33513982	3	38	theme	different	421:429	arg1	activities					503:512	anti-fungal and antibiofilm activities	475:512	activities	503:512	In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells.
33513982	3	38	theme	different	421:429	arg1	antibacterial					460:472	antibacterial	460:472	antibacterial	460:472	In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells.
33513982	3	38	theme	different	421:429	arg1	activities					442:451	different biological activities	421:451	different biological activities	421:451	In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells.
33513982	5	39	theme	Inhibitory	790:799	arg1	MIC					816:818	MIC	816:818	MIC	816:818	For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain.
33513982	5	39	theme	Inhibitory	790:799	arg1	Concentration					801:813	Minimum Inhibitory Concentration	782:813	Minimum Inhibitory Concentration (MIC)	782:819	For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain.
33513982	3	40	theme	cancer	598:603	arg1	cells					605:609	murine breast cancer cells	584:609	murine breast cancer cells	584:609	In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells.
33513982	4	41	theme	biological	665:674	arg1	activity					676:683	some biological activity	660:683	some biological activity	660:683	Results have shown that all EPS samples present some biological activity.
33513982	5	42	theme	better	759:764	arg1	efficiency					766:775	a better efficiency	757:775	a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL	757:849	For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain.
33513982	6	43	theme	Candida	890:896	arg1	albicans					898:905	Candida albicans	890:905	Candida albicans	890:905	For Candida albicans, the biofilm formation was reduced by about 90% by using only a 31.3 µg/mL concentration.
33513982	7	44	dep	55	1128:1129	arg1	to					1125:1126	to	1125:1126	to	1125:1126	Concerning breast cancer cells, lower molar masses fractions appeared to be more efficient, with a reduction of viability of up to 55%.
33513982	5	45	theme	bacterial	868:876	arg1	strain					878:883	the bacterial strain	864:883	the bacterial strain	864:883	For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain.
33513982	1	46	theme	generation	214:223	arg1	Exopolysaccharide					136:152	Exopolysaccharide	136:152	Exopolysaccharide (EPS) from marine microalgae	136:181	Exopolysaccharide (EPS) from marine microalgae are promising sources of a new generation of drugs.
33513982	1	46	theme	generation	214:223	arg1	sources					197:203	promising sources	187:203	promising sources of a new generation of drugs	187:232	Exopolysaccharide (EPS) from marine microalgae are promising sources of a new generation of drugs.
33513982	3	47	theme	murine	584:589	arg1	cells					605:609	murine breast cancer cells	584:609	murine breast cancer cells	584:609	In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells.
33513982	0	48	theme	Bacterial	73:81	arg1	Proliferation					83:95	Bacterial Proliferation	73:95	Bacterial Proliferation	73:95	Potential of Exopolysaccharide from Porphyridium marinum to Contend with Bacterial Proliferation, Biofilm Formation, and Breast Cancer.
33513982	1	49	theme	new	210:212	arg1	generation					214:223	a new generation	208:223	a new generation of drugs	208:232	Exopolysaccharide (EPS) from marine microalgae are promising sources of a new generation of drugs.
33513982	8	50	theme	viscosity	1179:1187	arg1	measurements					1189:1200	viscosity measurements	1179:1200	viscosity measurements	1179:1200	Finally, analyses of polymers composition and viscosity measurements were conducted on all samples, in order to propose hypotheses involving the activities caused by the intrinsic properties of polymers.
33513982	4	51	theme	EPS	640:642	arg1	samples					644:650	all EPS samples	636:650	all EPS samples	636:650	Results have shown that all EPS samples present some biological activity.
33513982	1	52	theme	drugs	228:232	arg1	generation					214:223	a new generation	208:223	a new generation of drugs	208:232	Exopolysaccharide (EPS) from marine microalgae are promising sources of a new generation of drugs.
33513982	3	53	dep	activities	442:451	arg1	i.e.					454:457	different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans	421:532	i.e.	454:457	In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells.
33513982	3	54	theme	antibiofilm	491:501	arg1	activities					503:512	anti-fungal and antibiofilm activities	475:512	activities	503:512	In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells.
33513982	3	54	theme	antibiofilm	491:501	arg1	activities					442:451	different biological activities	421:451	different biological activities	421:451	In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells.
33513982	3	55	from	activities	503:512	arg1	albicans					525:532	Candida albicans	517:532	Candida albicans	517:532	In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells.
33513982	5	56	theme	antibacterial	690:702	arg1	activities					720:729	antibacterial and antibiofilm activities	690:729	activities	720:729	For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain.
34564184	5	0	theme	few	1052:1054	arg1	peptides					1056:1063	few peptides	1052:1063	few peptides (1.72-6.77 wt%)	1052:1079	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	5	0	theme	few	1052:1054	arg1	acetyl					952:957	acetyl	952:957	acetyl (0.5-2.1 wt%)	952:971	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	5	0	theme	few	1052:1054	arg1	pyruvyl					974:980	pyruvyl	974:980	pyruvyl (0.2-4.9 wt%)	974:994	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	5	0	theme	few	1052:1054	arg1	succinyl					997:1004	succinyl	997:1004	succinyl (1-1.8 wt%)	997:1016	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	5	0	theme	few	1052:1054	arg1	%					1078:1078	1.72-6.77 wt%	1066:1078	1.72-6.77 wt%	1066:1078	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	5	0	theme	few	1052:1054	arg1	sulfate					1023:1029	sulfate	1023:1029	sulfate (1.98-3.43 wt%)	1023:1045	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	6	1	theme	high	1192:1195	arg1	yields					1202:1207	high char yields	1192:1207	high char yields (32-53%)	1192:1216	Thermal analysis demonstrated that the EPS had a degradation temperature above 260 °C, and high char yields (32-53%).
34564184	6	1	theme	high	1192:1195	arg1	%					1215:1215	32-53%	1210:1215	32-53%	1210:1215	Thermal analysis demonstrated that the EPS had a degradation temperature above 260 °C, and high char yields (32-53%).
34564184	7	2	from	Studies	1219:1225	arg1	properties					1245:1254	EPS functional properties	1230:1254	EPS functional properties	1230:1254	Studies on EPS functional properties revealed that they produce viscous aqueous solutions with a shear thinning behavior and could form strong gels in two distinct ways: by the addition of Fe2+, or in the presence of Mg2+, Cu2+, or Ca2+ under alkaline conditions.
34564184	3	3	theme	marine	552:557	arg1	environments					559:570	different marine environments	542:570	different marine environments in French Polynesia atolls	542:597	Six Alteromonas strains from different marine environments in French Polynesia atolls were selected for EPS extraction.
34564184	5	4	theme	wt	1042:1043	arg1	sulfate					1023:1029	sulfate	1023:1029	sulfate (1.98-3.43 wt%)	1023:1045	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	5	4	theme	wt	1042:1043	arg1	%					1044:1044	1.98-3.43 wt%	1032:1044	1.98-3.43 wt%	1032:1044	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	7	5	theme	distinct	1374:1381	arg1	ways					1383:1386	two distinct ways	1370:1386	two distinct ways	1370:1386	Studies on EPS functional properties revealed that they produce viscous aqueous solutions with a shear thinning behavior and could form strong gels in two distinct ways: by the addition of Fe2+, or in the presence of Mg2+, Cu2+, or Ca2+ under alkaline conditions.
34564184	4	6	dep	acids	805:809	arg1	acid					845:848	galacturonic acid	832:848	galacturonic acid	832:848	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	4	6	dep	acids	805:809	arg1	acids					805:809	uronic acids	798:809	uronic acids (glucuronic acid and galacturonic acid)	798:849	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	4	6	dep	acids	805:809	arg1	acid					823:826	glucuronic acid	812:826	glucuronic acid	812:826	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	0	7	theme	French	130:135	arg1	Environments					154:165	French Polynesia Marine Environments	130:165	French Polynesia Marine Environments	130:165	Characterization and Biotechnological Potential of Extracellular Polysaccharides Synthesized by Alteromonas Strains Isolated from French Polynesia Marine Environments.
34564184	5	8	theme	wt	991:992	arg1	%					993:993	0.2-4.9 wt%	983:993	0.2-4.9 wt%	983:993	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	5	8	theme	wt	991:992	arg1	pyruvyl					974:980	pyruvyl	974:980	pyruvyl (0.2-4.9 wt%)	974:994	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	4	9	theme	EPS	895:897	arg1	compositions					899:910	the EPS compositions	891:910	the EPS compositions	891:910	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	3	10	from	environments	559:570	arg1	strains					529:535	Six Alteromonas strains	513:535	Six Alteromonas strains from different marine environments in French Polynesia atolls	513:597	Six Alteromonas strains from different marine environments in French Polynesia atolls were selected for EPS extraction.
34564184	3	10	from	environments	559:570	arg1	atolls					592:597	French Polynesia atolls	575:597	French Polynesia atolls	575:597	Six Alteromonas strains from different marine environments in French Polynesia atolls were selected for EPS extraction.
34564184	7	11	theme	thinning	1322:1329	arg1	behavior					1331:1338	a shear thinning behavior	1314:1338	a shear thinning behavior	1314:1338	Studies on EPS functional properties revealed that they produce viscous aqueous solutions with a shear thinning behavior and could form strong gels in two distinct ways: by the addition of Fe2+, or in the presence of Mg2+, Cu2+, or Ca2+ under alkaline conditions.
34564184	7	12	theme	aqueous	1291:1297	arg1	solutions					1299:1307	viscous aqueous solutions	1283:1307	viscous aqueous solutions with a shear thinning behavior	1283:1338	Studies on EPS functional properties revealed that they produce viscous aqueous solutions with a shear thinning behavior and could form strong gels in two distinct ways: by the addition of Fe2+, or in the presence of Mg2+, Cu2+, or Ca2+ under alkaline conditions.
34564184	2	13	contain	have	400:403	arg2	potential					412:420	a huge potential	405:420	a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS)	405:510	The vast ecological and metabolic diversity found in marine microorganisms suggest that these marine resources have a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS).
34564184	2	13	contain	have	400:403	arg1	resources					390:398	these marine resources	377:398	these marine resources	377:398	The vast ecological and metabolic diversity found in marine microorganisms suggest that these marine resources have a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS).
34564184	7	14	theme	alkaline	1462:1469	arg1	conditions					1471:1480	alkaline conditions	1462:1480	alkaline conditions	1462:1480	Studies on EPS functional properties revealed that they produce viscous aqueous solutions with a shear thinning behavior and could form strong gels in two distinct ways: by the addition of Fe2+, or in the presence of Mg2+, Cu2+, or Ca2+ under alkaline conditions.
34564184	5	15	theme	Non-carbohydrate	913:928	arg1	sulfate					1023:1029	sulfate	1023:1029	sulfate (1.98-3.43 wt%)	1023:1045	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	5	15	theme	Non-carbohydrate	913:928	arg1	succinyl					997:1004	succinyl	997:1004	succinyl (1-1.8 wt%)	997:1016	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	5	15	theme	Non-carbohydrate	913:928	arg1	substituents					930:941	Non-carbohydrate substituents	913:941	Non-carbohydrate substituents	913:941	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	5	15	theme	Non-carbohydrate	913:928	arg1	pyruvyl					974:980	pyruvyl	974:980	pyruvyl (0.2-4.9 wt%)	974:994	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	5	15	theme	Non-carbohydrate	913:928	arg1	acetyl					952:957	acetyl	952:957	acetyl (0.5-2.1 wt%)	952:971	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	0	16	theme	Marine	147:152	arg1	Environments					154:165	French Polynesia Marine Environments	130:165	French Polynesia Marine Environments	130:165	Characterization and Biotechnological Potential of Extracellular Polysaccharides Synthesized by Alteromonas Strains Isolated from French Polynesia Marine Environments.
34564184	7	17	theme	strong	1355:1360	arg1	gels					1362:1365	strong gels	1355:1365	strong gels	1355:1365	Studies on EPS functional properties revealed that they produce viscous aqueous solutions with a shear thinning behavior and could form strong gels in two distinct ways: by the addition of Fe2+, or in the presence of Mg2+, Cu2+, or Ca2+ under alkaline conditions.
34564184	2	18	theme	metabolic	313:321	arg1	diversity					323:331	The vast ecological and metabolic diversity	289:331	The vast ecological and metabolic diversity found in marine microorganisms	289:362	The vast ecological and metabolic diversity found in marine microorganisms suggest that these marine resources have a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS).
34564184	8	19	theme	different	1532:1540	arg1	applications					1542:1553	different applications	1532:1553	different applications	1532:1553	Thus, these EPS could be versatile materials for different applications.
34564184	2	20	theme	novel	436:440	arg1	exopolysaccharides					487:504	exopolysaccharides	487:504	exopolysaccharides (EPS)	487:510	The vast ecological and metabolic diversity found in marine microorganisms suggest that these marine resources have a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS).
34564184	2	20	theme	novel	436:440	arg1	biomolecules					465:476	novel commercially appealing biomolecules	436:476	novel commercially appealing biomolecules	436:476	The vast ecological and metabolic diversity found in marine microorganisms suggest that these marine resources have a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS).
34564184	0	21	theme	Polynesia	137:145	arg1	Environments					154:165	French Polynesia Marine Environments	130:165	French Polynesia Marine Environments	130:165	Characterization and Biotechnological Potential of Extracellular Polysaccharides Synthesized by Alteromonas Strains Isolated from French Polynesia Marine Environments.
34564184	4	22	theme	glucuronic	812:821	arg1	acids					805:809	uronic acids	798:809	uronic acids (glucuronic acid and galacturonic acid)	798:849	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	4	22	theme	glucuronic	812:821	arg1	acid					823:826	glucuronic acid	812:826	glucuronic acid	812:826	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	4	23	dep	mol	883:885	arg1	45.5					878:881	45.5	878:881	45.5	878:881	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	5	24	theme	wt	968:969	arg1	%					970:970	0.5-2.1 wt%	960:970	0.5-2.1 wt%	960:970	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	5	24	theme	wt	968:969	arg1	acetyl					952:957	acetyl	952:957	acetyl (0.5-2.1 wt%)	952:971	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	4	25	dep	45.5	878:881	arg1	to					875:876	to	875:876	to	875:876	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	6	26	theme	degradation	1150:1160	arg1	temperature					1162:1172	a degradation temperature	1148:1172	a degradation temperature above 260 °C	1148:1185	Thermal analysis demonstrated that the EPS had a degradation temperature above 260 °C, and high char yields (32-53%).
34564184	7	27	theme	functional	1234:1243	arg1	properties					1245:1254	EPS functional properties	1230:1254	EPS functional properties	1230:1254	Studies on EPS functional properties revealed that they produce viscous aqueous solutions with a shear thinning behavior and could form strong gels in two distinct ways: by the addition of Fe2+, or in the presence of Mg2+, Cu2+, or Ca2+ under alkaline conditions.
34564184	1	28	theme	surface	230:236	arg1	quarters					210:217	almost three quarters	197:217	almost three quarters of Earth's surface	197:236	Marine environments comprise almost three quarters of Earth's surface, representing the largest ecosystem of our planet.
34564184	7	29	with	solutions	1299:1307	arg1	behavior					1331:1338	a shear thinning behavior	1314:1338	a shear thinning behavior	1314:1338	Studies on EPS functional properties revealed that they produce viscous aqueous solutions with a shear thinning behavior and could form strong gels in two distinct ways: by the addition of Fe2+, or in the presence of Mg2+, Cu2+, or Ca2+ under alkaline conditions.
34564184	7	30	theme	EPS	1230:1232	arg1	properties					1245:1254	EPS functional properties	1230:1254	EPS functional properties	1230:1254	Studies on EPS functional properties revealed that they produce viscous aqueous solutions with a shear thinning behavior and could form strong gels in two distinct ways: by the addition of Fe2+, or in the presence of Mg2+, Cu2+, or Ca2+ under alkaline conditions.
34564184	2	31	theme	marine	342:347	arg1	microorganisms					349:362	marine microorganisms	342:362	marine microorganisms	342:362	The vast ecological and metabolic diversity found in marine microorganisms suggest that these marine resources have a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS).
34564184	0	32	theme	Biotechnological	21:36	arg1	Potential					38:46	Biotechnological Potential	21:46	Biotechnological Potential	21:46	Characterization and Biotechnological Potential of Extracellular Polysaccharides Synthesized by Alteromonas Strains Isolated from French Polynesia Marine Environments.
34564184	6	33	contain	had	1144:1146	arg1	EPS					1140:1142	the EPS	1136:1142	the EPS	1136:1142	Thermal analysis demonstrated that the EPS had a degradation temperature above 260 °C, and high char yields (32-53%).
34564184	6	33	contain	had	1144:1146	arg2	temperature					1162:1172	a degradation temperature	1148:1172	a degradation temperature above 260 °C	1148:1185	Thermal analysis demonstrated that the EPS had a degradation temperature above 260 °C, and high char yields (32-53%).
34564184	6	33	contain	had	1144:1146	arg2	yields					1202:1207	high char yields	1192:1207	high char yields (32-53%)	1192:1216	Thermal analysis demonstrated that the EPS had a degradation temperature above 260 °C, and high char yields (32-53%).
34564184	6	33	contain	had	1144:1146	arg2	%					1215:1215	32-53%	1210:1215	32-53%	1210:1215	Thermal analysis demonstrated that the EPS had a degradation temperature above 260 °C, and high char yields (32-53%).
34564184	3	34	theme	Polynesia	582:590	arg1	atolls					592:597	French Polynesia atolls	575:597	French Polynesia atolls	575:597	Six Alteromonas strains from different marine environments in French Polynesia atolls were selected for EPS extraction.
34564184	7	35	theme	Fe2+	1408:1411	arg1	addition					1396:1403	the addition	1392:1403	the addition of Fe2+	1392:1411	Studies on EPS functional properties revealed that they produce viscous aqueous solutions with a shear thinning behavior and could form strong gels in two distinct ways: by the addition of Fe2+, or in the presence of Mg2+, Cu2+, or Ca2+ under alkaline conditions.
34564184	3	36	theme	EPS	617:619	arg1	extraction					621:630	EPS extraction	617:630	EPS extraction	617:630	Six Alteromonas strains from different marine environments in French Polynesia atolls were selected for EPS extraction.
34564184	2	37	theme	biomolecules	465:476	arg1	sources					425:431	sources	425:431	sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS)	425:510	The vast ecological and metabolic diversity found in marine microorganisms suggest that these marine resources have a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS).
34564184	0	38	theme	Extracellular	51:63	arg1	Polysaccharides					65:79	Extracellular Polysaccharides	51:79	Extracellular Polysaccharides	51:79	Characterization and Biotechnological Potential of Extracellular Polysaccharides Synthesized by Alteromonas Strains Isolated from French Polynesia Marine Environments.
34564184	3	39	theme	different	542:550	arg1	environments					559:570	different marine environments	542:570	different marine environments in French Polynesia atolls	542:597	Six Alteromonas strains from different marine environments in French Polynesia atolls were selected for EPS extraction.
34564184	4	40	theme	neutral	714:720	arg1	rhamnose					772:779	rhamnose	772:779	rhamnose	772:779	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	4	40	theme	neutral	714:720	arg1	fucose					785:790	fucose	785:790	fucose	785:790	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	4	40	theme	neutral	714:720	arg1	galactose					748:756	galactose	748:756	galactose	748:756	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	4	40	theme	neutral	714:720	arg1	mannose					763:769	mannose	763:769	mannose	763:769	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	4	40	theme	neutral	714:720	arg1	monosaccharides					722:736	neutral monosaccharides	714:736	neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose)	714:791	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	4	40	theme	neutral	714:720	arg1	glucose					739:745	glucose	739:745	glucose	739:745	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	2	41	theme	appealing	455:463	arg1	exopolysaccharides					487:504	exopolysaccharides	487:504	exopolysaccharides (EPS)	487:510	The vast ecological and metabolic diversity found in marine microorganisms suggest that these marine resources have a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS).
34564184	2	41	theme	appealing	455:463	arg1	biomolecules					465:476	novel commercially appealing biomolecules	436:476	novel commercially appealing biomolecules	436:476	The vast ecological and metabolic diversity found in marine microorganisms suggest that these marine resources have a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS).
34564184	7	42	theme	Ca2+	1451:1454	arg1	presence					1424:1431	the presence	1420:1431	the presence of Mg2+, Cu2+, or Ca2+	1420:1454	Studies on EPS functional properties revealed that they produce viscous aqueous solutions with a shear thinning behavior and could form strong gels in two distinct ways: by the addition of Fe2+, or in the presence of Mg2+, Cu2+, or Ca2+ under alkaline conditions.
34564184	7	43	theme	viscous	1283:1289	arg1	solutions					1299:1307	viscous aqueous solutions	1283:1307	viscous aqueous solutions with a shear thinning behavior	1283:1338	Studies on EPS functional properties revealed that they produce viscous aqueous solutions with a shear thinning behavior and could form strong gels in two distinct ways: by the addition of Fe2+, or in the presence of Mg2+, Cu2+, or Ca2+ under alkaline conditions.
34564184	1	44	theme	largest	256:262	arg1	ecosystem					264:272	the largest ecosystem	252:272	the largest ecosystem of our planet	252:286	Marine environments comprise almost three quarters of Earth's surface, representing the largest ecosystem of our planet.
34564184	2	45	located	found	333:337	arg1	microorganisms					349:362	marine microorganisms	342:362	marine microorganisms	342:362	The vast ecological and metabolic diversity found in marine microorganisms suggest that these marine resources have a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS).
34564184	2	45	located	found	333:337	arg2	diversity					323:331	The vast ecological and metabolic diversity	289:331	The vast ecological and metabolic diversity found in marine microorganisms	289:362	The vast ecological and metabolic diversity found in marine microorganisms suggest that these marine resources have a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS).
34564184	7	46	theme	Cu2+	1442:1445	arg1	presence					1424:1431	the presence	1420:1431	the presence of Mg2+, Cu2+, or Ca2+	1420:1454	Studies on EPS functional properties revealed that they produce viscous aqueous solutions with a shear thinning behavior and could form strong gels in two distinct ways: by the addition of Fe2+, or in the presence of Mg2+, Cu2+, or Ca2+ under alkaline conditions.
34564184	0	47	theme	Polysaccharides	65:79	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and Biotechnological Potential of Extracellular Polysaccharides Synthesized by Alteromonas Strains Isolated from French Polynesia Marine Environments.
34564184	0	47	theme	Polysaccharides	65:79	arg1	Potential					38:46	Biotechnological Potential	21:46	Biotechnological Potential	21:46	Characterization and Biotechnological Potential of Extracellular Polysaccharides Synthesized by Alteromonas Strains Isolated from French Polynesia Marine Environments.
34564184	4	48	theme	compositions	899:910	arg1	compositions					899:910	the EPS compositions	891:910	the EPS compositions	891:910	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	4	48	theme	compositions	899:910	arg1	%					886:886	up to 45.5 mol%	872:886	up to 45.5 mol% of the EPS compositions	872:910	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	5	49	theme	wt	1013:1014	arg1	succinyl					997:1004	succinyl	997:1004	succinyl (1-1.8 wt%)	997:1016	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	5	49	theme	wt	1013:1014	arg1	%					1015:1015	1-1.8 wt%	1007:1015	1-1.8 wt%	1007:1015	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	2	50	theme	marine	383:388	arg1	resources					390:398	these marine resources	377:398	these marine resources	377:398	The vast ecological and metabolic diversity found in marine microorganisms suggest that these marine resources have a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS).
34564184	6	51	theme	Thermal	1101:1107	arg1	analysis					1109:1116	Thermal analysis	1101:1116	Thermal analysis	1101:1116	Thermal analysis demonstrated that the EPS had a degradation temperature above 260 °C, and high char yields (32-53%).
34564184	7	52	theme	shear	1316:1320	arg1	behavior					1331:1338	a shear thinning behavior	1314:1338	a shear thinning behavior	1314:1338	Studies on EPS functional properties revealed that they produce viscous aqueous solutions with a shear thinning behavior and could form strong gels in two distinct ways: by the addition of Fe2+, or in the presence of Mg2+, Cu2+, or Ca2+ under alkaline conditions.
34564184	4	53	theme	galacturonic	832:843	arg1	acid					845:848	galacturonic acid	832:848	galacturonic acid	832:848	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	4	53	theme	galacturonic	832:843	arg1	acids					805:809	uronic acids	798:809	uronic acids (glucuronic acid and galacturonic acid)	798:849	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	2	54	theme	ecological	298:307	arg1	diversity					323:331	The vast ecological and metabolic diversity	289:331	The vast ecological and metabolic diversity found in marine microorganisms	289:362	The vast ecological and metabolic diversity found in marine microorganisms suggest that these marine resources have a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS).
34564184	0	55	attach	Isolated	116:123	arg1	Environments					154:165	French Polynesia Marine Environments	130:165	French Polynesia Marine Environments	130:165	Characterization and Biotechnological Potential of Extracellular Polysaccharides Synthesized by Alteromonas Strains Isolated from French Polynesia Marine Environments.
34564184	0	55	attach	Isolated	116:123	arg2	Strains					108:114	Alteromonas Strains	96:114	Alteromonas Strains Isolated from French Polynesia Marine Environments	96:165	Characterization and Biotechnological Potential of Extracellular Polysaccharides Synthesized by Alteromonas Strains Isolated from French Polynesia Marine Environments.
34564184	1	56	theme	Marine	168:173	arg1	environments					175:186	Marine environments	168:186	Marine environments	168:186	Marine environments comprise almost three quarters of Earth's surface, representing the largest ecosystem of our planet.
34564184	4	57	theme	uronic	798:803	arg1	acid					845:848	galacturonic acid	832:848	galacturonic acid	832:848	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	4	57	theme	uronic	798:803	arg1	acids					805:809	uronic acids	798:809	uronic acids (glucuronic acid and galacturonic acid)	798:849	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	4	57	theme	uronic	798:803	arg1	acid					823:826	glucuronic acid	812:826	glucuronic acid	812:826	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	6	58	theme	char	1197:1200	arg1	yields					1202:1207	high char yields	1192:1207	high char yields (32-53%)	1192:1216	Thermal analysis demonstrated that the EPS had a degradation temperature above 260 °C, and high char yields (32-53%).
34564184	6	58	theme	char	1197:1200	arg1	%					1215:1215	32-53%	1210:1215	32-53%	1210:1215	Thermal analysis demonstrated that the EPS had a degradation temperature above 260 °C, and high char yields (32-53%).
34564184	2	59	theme	vast	293:296	arg1	diversity					323:331	The vast ecological and metabolic diversity	289:331	The vast ecological and metabolic diversity found in marine microorganisms	289:362	The vast ecological and metabolic diversity found in marine microorganisms suggest that these marine resources have a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS).
34564184	4	60	dep	%	886:886	arg1	mol					883:885	mol	883:885	mol	883:885	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	3	61	theme	French	575:580	arg1	atolls					592:597	French Polynesia atolls	575:597	French Polynesia atolls	575:597	Six Alteromonas strains from different marine environments in French Polynesia atolls were selected for EPS extraction.
34564184	3	62	theme	Alteromonas	517:527	arg1	strains					529:535	Six Alteromonas strains	513:535	Six Alteromonas strains from different marine environments in French Polynesia atolls	513:597	Six Alteromonas strains from different marine environments in French Polynesia atolls were selected for EPS extraction.
34564184	0	63	theme	Alteromonas	96:106	arg1	Strains					108:114	Alteromonas Strains	96:114	Alteromonas Strains Isolated from French Polynesia Marine Environments	96:165	Characterization and Biotechnological Potential of Extracellular Polysaccharides Synthesized by Alteromonas Strains Isolated from French Polynesia Marine Environments.
34564184	7	64	theme	Mg2+	1436:1439	arg1	presence					1424:1431	the presence	1420:1431	the presence of Mg2+, Cu2+, or Ca2+	1420:1454	Studies on EPS functional properties revealed that they produce viscous aqueous solutions with a shear thinning behavior and could form strong gels in two distinct ways: by the addition of Fe2+, or in the presence of Mg2+, Cu2+, or Ca2+ under alkaline conditions.
34564184	4	65	theme	different	684:692	arg1	monomers					694:701	different monomers	684:701	different monomers	684:701	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	5	66	theme	wt	1076:1077	arg1	peptides					1056:1063	few peptides	1052:1063	few peptides (1.72-6.77 wt%)	1052:1079	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	5	66	theme	wt	1076:1077	arg1	acetyl					952:957	acetyl	952:957	acetyl (0.5-2.1 wt%)	952:971	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	5	66	theme	wt	1076:1077	arg1	pyruvyl					974:980	pyruvyl	974:980	pyruvyl (0.2-4.9 wt%)	974:994	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	5	66	theme	wt	1076:1077	arg1	succinyl					997:1004	succinyl	997:1004	succinyl (1-1.8 wt%)	997:1016	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	5	66	theme	wt	1076:1077	arg1	%					1078:1078	1.72-6.77 wt%	1066:1078	1.72-6.77 wt%	1066:1078	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	5	66	theme	wt	1076:1077	arg1	sulfate					1023:1029	sulfate	1023:1029	sulfate (1.98-3.43 wt%)	1023:1045	Non-carbohydrate substituents, such as acetyl (0.5-2.1 wt%), pyruvyl (0.2-4.9 wt%), succinyl (1-1.8 wt%), and sulfate (1.98-3.43 wt%); and few peptides (1.72-6.77 wt%) were also detected.
34564184	4	67	dep	monosaccharides	722:736	arg1	galactose					748:756	galactose	748:756	galactose	748:756	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	4	67	dep	monosaccharides	722:736	arg1	mannose					763:769	mannose	763:769	mannose	763:769	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	4	67	dep	monosaccharides	722:736	arg1	glucose					739:745	glucose	739:745	glucose	739:745	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	4	67	dep	monosaccharides	722:736	arg1	monosaccharides					722:736	neutral monosaccharides	714:736	neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose)	714:791	All the EPS were heteropolysaccharides composed of different monomers, including neutral monosaccharides (glucose, galactose, and mannose, rhamnose and fucose), and uronic acids (glucuronic acid and galacturonic acid), which accounted for up to 45.5 mol% of the EPS compositions.
34564184	2	68	theme	huge	407:410	arg1	potential					412:420	a huge potential	405:420	a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS)	405:510	The vast ecological and metabolic diversity found in marine microorganisms suggest that these marine resources have a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS).
34564184	8	69	theme	versatile	1508:1516	arg1	materials					1518:1526	versatile materials	1508:1526	versatile materials for different applications	1508:1553	Thus, these EPS could be versatile materials for different applications.
34564184	1	70	theme	planet	281:286	arg1	ecosystem					264:272	the largest ecosystem	252:272	the largest ecosystem of our planet	252:286	Marine environments comprise almost three quarters of Earth's surface, representing the largest ecosystem of our planet.
32539334	8	0	theme	detailed	1609:1616	arg1	analysis					1618:1625	The detailed analysis	1605:1625	The detailed analysis presented here	1605:1640	The detailed analysis presented here allows identifying hydrogels for cell growth applications, while on the other hand, material properties can be predicted, and their overall number can be minimized leading to efficient optimization of bone reconstruction and other cell growth applications.
32539334	8	1	theme	bone	1843:1846	arg1	reconstruction					1848:1861	bone reconstruction	1843:1861	bone reconstruction	1843:1861	The detailed analysis presented here allows identifying hydrogels for cell growth applications, while on the other hand, material properties can be predicted, and their overall number can be minimized leading to efficient optimization of bone reconstruction and other cell growth applications.
32539334	6	2	theme	key	961:963	arg1	parameters					965:974	key parameters	961:974	key parameters	961:974	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	3	theme	production	1278:1287	arg1	rate					1249:1252	rate	1249:1252	rate of alkaline phosphatase production	1249:1287	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	3	theme	production	1278:1287	arg1	numbers					1227:1233	numbers	1227:1233	numbers of the cells	1227:1246	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	3	theme	production	1278:1287	arg1	area					1300:1303	cells area	1294:1303	cells area	1294:1303	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	3	theme	production	1278:1287	arg1	viability					1216:1224	viability	1216:1224	viability	1216:1224	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	7	4	theme	hydroxyapatite	1463:1476	arg1	formation					1478:1486	the hydroxyapatite formation	1459:1486	the hydroxyapatite formation	1459:1486	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	7	5	theme	ions	1428:1431	arg1	particles					1384:1392	the particles size and concentration	1380:1415	the particles size and concentration of calcium ions	1380:1431	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	7	5	theme	ions	1428:1431	arg1	concentration					1403:1415	concentration	1403:1415	concentration	1403:1415	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	7	5	theme	ions	1428:1431	arg1	size					1394:1397	size	1394:1397	size	1394:1397	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	4	6	theme	tissue	691:696	arg1	regeneration					698:709	bone tissue regeneration	686:709	bone tissue regeneration	686:709	Composite hydrogels could be used for bone tissue regeneration, but they exhibit poor ossification properties.
32539334	6	7	theme	alkaline	1257:1264	arg1	production					1278:1287	alkaline phosphatase production	1257:1287	alkaline phosphatase production	1257:1287	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	5	8	theme	Ca/Mg	907:911	arg1	particles					933:941	Ca/Mg carbonate submicron particles	907:941	Ca/Mg carbonate submicron particles	907:941	In current work, we have designed new osteoinductive gellan gum hydrogels by a thermal annealing approach and consequently functionalized them with Ca/Mg carbonate submicron particles.
32539334	7	9	from	effect	1449:1454	arg1	formation					1478:1486	the hydroxyapatite formation	1459:1486	the hydroxyapatite formation	1459:1486	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	8	10	theme	growth	1878:1883	arg1	applications					1885:1896	other cell growth applications	1867:1896	other cell growth applications	1867:1896	The detailed analysis presented here allows identifying hydrogels for cell growth applications, while on the other hand, material properties can be predicted, and their overall number can be minimized leading to efficient optimization of bone reconstruction and other cell growth applications.
32539334	1	11	theme	adhesion	205:212	arg1	mechanisms					186:195	the mechanisms	182:195	the mechanisms of cell adhesion	182:212	Developing materials for tissue engineering and studying the mechanisms of cell adhesion is a complex and multifactor process that needs analysis using physical chemistry and biology.
32539334	6	12	theme	cell	1173:1176	arg1	behavior					1178:1185	cell behavior	1173:1185	cell behavior	1173:1185	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	13	theme	composite	1338:1346	arg1	hydrogel					1348:1355	such composite hydrogel	1333:1355	such composite hydrogel	1333:1355	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	2	14	theme	major	313:317	arg1	challenge					319:327	The major challenge	309:327	The major challenge	309:327	The major challenge is the labor-intensive data mining as well as requirements of the number of advanced techniques.
32539334	2	14	theme	major	313:317	arg1	data					352:355	the labor-intensive data	332:355	the labor-intensive data mining as well as requirements of the number of advanced techniques	332:423	The major challenge is the labor-intensive data mining as well as requirements of the number of advanced techniques.
32539334	8	15	theme	material	1726:1733	arg1	properties					1735:1744	material properties	1726:1744	material properties	1726:1744	The detailed analysis presented here allows identifying hydrogels for cell growth applications, while on the other hand, material properties can be predicted, and their overall number can be minimized leading to efficient optimization of bone reconstruction and other cell growth applications.
32539334	3	16	theme	tissue	628:633	arg1	engineering					635:645	tissue engineering	628:645	tissue engineering	628:645	For example, hydrogel-based biomaterials with cell-binding sites, tunable mechanical properties, and complex architectures have emerged as a powerful tool to control cell adhesion and proliferation for tissue engineering.
32539334	5	17	theme	carbonate	913:921	arg1	particles					933:941	Ca/Mg carbonate submicron particles	907:941	Ca/Mg carbonate submicron particles	907:941	In current work, we have designed new osteoinductive gellan gum hydrogels by a thermal annealing approach and consequently functionalized them with Ca/Mg carbonate submicron particles.
32539334	0	18	from	Ossification	54:65	arg1	Materials					114:122	Particle Functionalized Composites Hydrogel Materials	70:122	Particle Functionalized Composites Hydrogel Materials	70:122	Identification and Analysis of Key Parameters for the Ossification on Particle Functionalized Composites Hydrogel Materials.
32539334	7	19	theme	cell	1590:1593	arg1	adhesion					1595:1602	cell adhesion	1590:1602	cell adhesion	1590:1602	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	4	20	used	used	677:680	arg2	hydrogels					658:666	Composite hydrogels	648:666	Composite hydrogels	648:666	Composite hydrogels could be used for bone tissue regeneration, but they exhibit poor ossification properties.
32539334	1	21	theme	tissue	150:155	arg1	engineering					157:167	tissue engineering	150:167	tissue engineering	150:167	Developing materials for tissue engineering and studying the mechanisms of cell adhesion is a complex and multifactor process that needs analysis using physical chemistry and biology.
32539334	8	22	theme	overall	1774:1780	arg1	number					1782:1787	their overall number	1768:1787	their overall number	1768:1787	The detailed analysis presented here allows identifying hydrogels for cell growth applications, while on the other hand, material properties can be predicted, and their overall number can be minimized leading to efficient optimization of bone reconstruction and other cell growth applications.
32539334	7	23	theme	desirable	1567:1575	arg1	property					1577:1584	a desirable property	1565:1584	a desirable property for cell adhesion	1565:1602	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	4	24	theme	poor	729:732	arg1	properties					747:756	poor ossification properties	729:756	poor ossification properties	729:756	Composite hydrogels could be used for bone tissue regeneration, but they exhibit poor ossification properties.
32539334	0	25	theme	Particle	70:77	arg1	Materials					114:122	Particle Functionalized Composites Hydrogel Materials	70:122	Particle Functionalized Composites Hydrogel Materials	70:122	Identification and Analysis of Key Parameters for the Ossification on Particle Functionalized Composites Hydrogel Materials.
32539334	8	26	theme	reconstruction	1848:1861	arg1	optimization					1827:1838	efficient optimization	1817:1838	efficient optimization of bone reconstruction and other cell growth applications	1817:1896	The detailed analysis presented here allows identifying hydrogels for cell growth applications, while on the other hand, material properties can be predicted, and their overall number can be minimized leading to efficient optimization of bone reconstruction and other cell growth applications.
32539334	6	27	theme	principal	1050:1058	arg1	analysis					1070:1077	the principal component analysis	1046:1077	the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior	1046:1185	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	0	28	theme	Composites	94:103	arg1	Materials					114:122	Particle Functionalized Composites Hydrogel Materials	70:122	Particle Functionalized Composites Hydrogel Materials	70:122	Identification and Analysis of Key Parameters for the Ossification on Particle Functionalized Composites Hydrogel Materials.
32539334	2	29	theme	advanced	405:412	arg1	techniques					414:423	advanced techniques	405:423	advanced techniques	405:423	The major challenge is the labor-intensive data mining as well as requirements of the number of advanced techniques.
32539334	0	30	theme	Parameters	35:44	arg1	Analysis					19:26	Analysis	19:26	Analysis	19:26	Identification and Analysis of Key Parameters for the Ossification on Particle Functionalized Composites Hydrogel Materials.
32539334	0	30	theme	Parameters	35:44	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification and Analysis of Key Parameters for the Ossification on Particle Functionalized Composites Hydrogel Materials.
32539334	6	31	theme	hydrogel	1120:1127	arg1	modulus					1105:1111	Young's modulus	1097:1111	Young's modulus of the hydrogel and particles	1097:1141	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	31	theme	hydrogel	1120:1127	arg1	mass					1163:1166	mass	1163:1166	mass	1163:1166	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	31	theme	hydrogel	1120:1127	arg1	size					1153:1156	particle size	1144:1156	particle size	1144:1156	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	31	theme	hydrogel	1120:1127	arg1	parameters					1085:1094	18 parameters	1082:1094	18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass)	1082:1167	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	2	32	theme	number	395:400	arg1	challenge					319:327	The major challenge	309:327	The major challenge	309:327	The major challenge is the labor-intensive data mining as well as requirements of the number of advanced techniques.
32539334	2	32	theme	number	395:400	arg1	requirements					375:386	requirements	375:386	the labor-intensive data mining as well as requirements of the number of advanced techniques	332:423	The major challenge is the labor-intensive data mining as well as requirements of the number of advanced techniques.
32539334	2	32	theme	number	395:400	arg1	data					352:355	the labor-intensive data	332:355	the labor-intensive data mining as well as requirements of the number of advanced techniques	332:423	The major challenge is the labor-intensive data mining as well as requirements of the number of advanced techniques.
32539334	5	33	theme	thermal	838:844	arg1	approach					856:863	a thermal annealing approach	836:863	a thermal annealing approach	836:863	In current work, we have designed new osteoinductive gellan gum hydrogels by a thermal annealing approach and consequently functionalized them with Ca/Mg carbonate submicron particles.
32539334	8	34	theme	cell	1873:1876	arg1	applications					1885:1896	other cell growth applications	1867:1896	other cell growth applications	1867:1896	The detailed analysis presented here allows identifying hydrogels for cell growth applications, while on the other hand, material properties can be predicted, and their overall number can be minimized leading to efficient optimization of bone reconstruction and other cell growth applications.
32539334	6	35	theme	time	1198:1201	arg1	points					1203:1208	various time points	1190:1208	various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel	1190:1355	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	7	36	theme	Young	1534:1538	arg1	modulus					1542:1548	a high Young's modulus	1527:1548	a high Young's modulus in a hydrogel	1527:1562	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	3	37	theme	cell-binding	472:483	arg1	sites					485:489	cell-binding sites	472:489	cell-binding sites	472:489	For example, hydrogel-based biomaterials with cell-binding sites, tunable mechanical properties, and complex architectures have emerged as a powerful tool to control cell adhesion and proliferation for tissue engineering.
32539334	0	38	theme	Key	31:33	arg1	Parameters					35:44	Key Parameters	31:44	Key Parameters for the Ossification on Particle Functionalized Composites Hydrogel Materials	31:122	Identification and Analysis of Key Parameters for the Ossification on Particle Functionalized Composites Hydrogel Materials.
32539334	7	39	contain	have	1433:1436	arg2	property					1577:1584	a desirable property	1565:1584	a desirable property for cell adhesion	1565:1602	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	7	39	contain	have	1433:1436	arg2	effect					1449:1454	a dominant effect	1438:1454	a dominant effect on the hydroxyapatite formation	1438:1486	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	7	39	contain	have	1433:1436	arg1	particles					1384:1392	the particles size and concentration	1380:1415	the particles size and concentration of calcium ions	1380:1431	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	7	39	contain	have	1433:1436	arg1	concentration					1403:1415	concentration	1403:1415	concentration	1403:1415	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	7	39	contain	have	1433:1436	arg1	size					1394:1397	size	1394:1397	size	1394:1397	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	5	40	theme	gum	819:821	arg1	hydrogels					823:831	new osteoinductive gellan gum hydrogels	793:831	new osteoinductive gellan gum hydrogels	793:831	In current work, we have designed new osteoinductive gellan gum hydrogels by a thermal annealing approach and consequently functionalized them with Ca/Mg carbonate submicron particles.
32539334	8	41	theme	other	1867:1871	arg1	applications					1885:1896	other cell growth applications	1867:1896	other cell growth applications	1867:1896	The detailed analysis presented here allows identifying hydrogels for cell growth applications, while on the other hand, material properties can be predicted, and their overall number can be minimized leading to efficient optimization of bone reconstruction and other cell growth applications.
32539334	3	42	theme	mechanical	500:509	arg1	properties					511:520	tunable mechanical properties	492:520	tunable mechanical properties	492:520	For example, hydrogel-based biomaterials with cell-binding sites, tunable mechanical properties, and complex architectures have emerged as a powerful tool to control cell adhesion and proliferation for tissue engineering.
32539334	1	43	theme	multifactor	231:241	arg1	process					243:249	a complex and multifactor process	217:249	a complex and multifactor process that needs analysis using physical chemistry and biology	217:306	Developing materials for tissue engineering and studying the mechanisms of cell adhesion is a complex and multifactor process that needs analysis using physical chemistry and biology.
32539334	7	44	theme	providing	1500:1508	arg1	areas					1516:1520	providing local areas	1500:1520	providing local areas with a high Young's modulus in a hydrogel	1500:1562	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	8	45	theme	identifying	1649:1659	arg1	hydrogels					1661:1669	identifying hydrogels	1649:1669	identifying hydrogels	1649:1669	The detailed analysis presented here allows identifying hydrogels for cell growth applications, while on the other hand, material properties can be predicted, and their overall number can be minimized leading to efficient optimization of bone reconstruction and other cell growth applications.
32539334	6	46	theme	particle	1144:1151	arg1	size					1153:1156	particle size	1144:1156	particle size	1144:1156	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	46	theme	particle	1144:1151	arg1	parameters					1085:1094	18 parameters	1082:1094	18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass)	1082:1167	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	4	47	theme	Composite	648:656	arg1	hydrogels					658:666	Composite hydrogels	648:666	Composite hydrogels	648:666	Composite hydrogels could be used for bone tissue regeneration, but they exhibit poor ossification properties.
32539334	5	48	theme	new	793:795	arg1	hydrogels					823:831	new osteoinductive gellan gum hydrogels	793:831	new osteoinductive gellan gum hydrogels	793:831	In current work, we have designed new osteoinductive gellan gum hydrogels by a thermal annealing approach and consequently functionalized them with Ca/Mg carbonate submicron particles.
32539334	6	49	theme	cells	1294:1298	arg1	area					1300:1303	cells area	1294:1303	cells area	1294:1303	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	3	50	theme	complex	527:533	arg1	architectures					535:547	complex architectures	527:547	complex architectures	527:547	For example, hydrogel-based biomaterials with cell-binding sites, tunable mechanical properties, and complex architectures have emerged as a powerful tool to control cell adhesion and proliferation for tissue engineering.
32539334	6	51	theme	parameters	965:974	arg1	Determination					944:956	Determination	944:956	Determination	944:956	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	52	theme	particles	1133:1141	arg1	modulus					1105:1111	Young's modulus	1097:1111	Young's modulus of the hydrogel and particles	1097:1141	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	52	theme	particles	1133:1141	arg1	mass					1163:1166	mass	1163:1166	mass	1163:1166	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	52	theme	particles	1133:1141	arg1	size					1153:1156	particle size	1144:1156	particle size	1144:1156	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	52	theme	particles	1133:1141	arg1	parameters					1085:1094	18 parameters	1082:1094	18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass)	1082:1167	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	3	53	theme	powerful	567:574	arg1	tool					576:579	a powerful tool	565:579	a powerful tool to control cell adhesion and proliferation for tissue engineering	565:645	For example, hydrogel-based biomaterials with cell-binding sites, tunable mechanical properties, and complex architectures have emerged as a powerful tool to control cell adhesion and proliferation for tissue engineering.
32539334	8	54	theme	growth	1680:1685	arg1	applications					1687:1698	cell growth applications	1675:1698	cell growth applications	1675:1698	The detailed analysis presented here allows identifying hydrogels for cell growth applications, while on the other hand, material properties can be predicted, and their overall number can be minimized leading to efficient optimization of bone reconstruction and other cell growth applications.
32539334	1	55	theme	physical	277:284	arg1	chemistry					286:294	physical chemistry	277:294	physical chemistry	277:294	Developing materials for tissue engineering and studying the mechanisms of cell adhesion is a complex and multifactor process that needs analysis using physical chemistry and biology.
32539334	7	56	theme	dominant	1440:1447	arg1	effect					1449:1454	a dominant effect	1438:1454	a dominant effect on the hydroxyapatite formation	1438:1486	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	6	57	theme	phosphatase	1266:1276	arg1	production					1278:1287	alkaline phosphatase production	1257:1287	alkaline phosphatase production	1257:1287	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	1	58	theme	cell	200:203	arg1	adhesion					205:212	cell adhesion	200:212	cell adhesion	200:212	Developing materials for tissue engineering and studying the mechanisms of cell adhesion is a complex and multifactor process that needs analysis using physical chemistry and biology.
32539334	6	59	theme	various	1190:1196	arg1	points					1203:1208	various time points	1190:1208	various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel	1190:1355	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	4	60	theme	bone	686:689	arg1	regeneration					698:709	bone tissue regeneration	686:709	bone tissue regeneration	686:709	Composite hydrogels could be used for bone tissue regeneration, but they exhibit poor ossification properties.
32539334	5	61	theme	osteoinductive	797:810	arg1	hydrogels					823:831	new osteoinductive gellan gum hydrogels	793:831	new osteoinductive gellan gum hydrogels	793:831	In current work, we have designed new osteoinductive gellan gum hydrogels by a thermal annealing approach and consequently functionalized them with Ca/Mg carbonate submicron particles.
32539334	8	62	theme	applications	1885:1896	arg1	optimization					1827:1838	efficient optimization	1817:1838	efficient optimization of bone reconstruction and other cell growth applications	1817:1896	The detailed analysis presented here allows identifying hydrogels for cell growth applications, while on the other hand, material properties can be predicted, and their overall number can be minimized leading to efficient optimization of bone reconstruction and other cell growth applications.
32539334	3	63	theme	cell	592:595	arg1	adhesion					597:604	cell adhesion	592:604	cell adhesion	592:604	For example, hydrogel-based biomaterials with cell-binding sites, tunable mechanical properties, and complex architectures have emerged as a powerful tool to control cell adhesion and proliferation for tissue engineering.
32539334	7	64	dep	particles	1384:1392	arg1	particles					1384:1392	the particles size and concentration	1380:1415	the particles size and concentration of calcium ions	1380:1431	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	7	64	dep	particles	1384:1392	arg1	concentration					1403:1415	concentration	1403:1415	concentration	1403:1415	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	7	64	dep	particles	1384:1392	arg1	size					1394:1397	size	1394:1397	size	1394:1397	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	6	65	theme	behavior	1178:1185	arg1	analysis					1070:1077	the principal component analysis	1046:1077	the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior	1046:1185	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	8	66	theme	other	1714:1718	arg1	hand					1720:1723	the other hand	1710:1723	the other hand	1710:1723	The detailed analysis presented here allows identifying hydrogels for cell growth applications, while on the other hand, material properties can be predicted, and their overall number can be minimized leading to efficient optimization of bone reconstruction and other cell growth applications.
32539334	7	67	theme	calcium	1420:1426	arg1	ions					1428:1431	calcium ions	1420:1431	calcium ions	1420:1431	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	6	68	theme	such	1333:1336	arg1	hydrogel					1348:1355	such composite hydrogel	1333:1355	such composite hydrogel	1333:1355	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	5	69	theme	submicron	923:931	arg1	particles					933:941	Ca/Mg carbonate submicron particles	907:941	Ca/Mg carbonate submicron particles	907:941	In current work, we have designed new osteoinductive gellan gum hydrogels by a thermal annealing approach and consequently functionalized them with Ca/Mg carbonate submicron particles.
32539334	1	70	theme	complex	219:225	arg1	process					243:249	a complex and multifactor process	217:249	a complex and multifactor process that needs analysis using physical chemistry and biology	217:306	Developing materials for tissue engineering and studying the mechanisms of cell adhesion is a complex and multifactor process that needs analysis using physical chemistry and biology.
32539334	6	71	theme	hydroxyapatite	1006:1019	arg1	generation					1021:1030	a successful hydroxyapatite generation	993:1030	a successful hydroxyapatite generation	993:1030	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	72	theme	cells	1242:1246	arg1	rate					1249:1252	rate	1249:1252	rate of alkaline phosphatase production	1249:1287	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	72	theme	cells	1242:1246	arg1	numbers					1227:1233	numbers	1227:1233	numbers of the cells	1227:1246	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	72	theme	cells	1242:1246	arg1	area					1300:1303	cells area	1294:1303	cells area	1294:1303	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	72	theme	cells	1242:1246	arg1	viability					1216:1224	viability	1216:1224	viability	1216:1224	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	5	73	theme	current	762:768	arg1	work					770:773	current work	762:773	current work	762:773	In current work, we have designed new osteoinductive gellan gum hydrogels by a thermal annealing approach and consequently functionalized them with Ca/Mg carbonate submicron particles.
32539334	4	74	theme	ossification	734:745	arg1	properties					747:756	poor ossification properties	729:756	poor ossification properties	729:756	Composite hydrogels could be used for bone tissue regeneration, but they exhibit poor ossification properties.
32539334	3	75	with	biomaterials	454:465	arg1	architectures					535:547	complex architectures	527:547	complex architectures	527:547	For example, hydrogel-based biomaterials with cell-binding sites, tunable mechanical properties, and complex architectures have emerged as a powerful tool to control cell adhesion and proliferation for tissue engineering.
32539334	3	75	with	biomaterials	454:465	arg1	properties					511:520	tunable mechanical properties	492:520	tunable mechanical properties	492:520	For example, hydrogel-based biomaterials with cell-binding sites, tunable mechanical properties, and complex architectures have emerged as a powerful tool to control cell adhesion and proliferation for tissue engineering.
32539334	3	75	with	biomaterials	454:465	arg1	sites					485:489	cell-binding sites	472:489	cell-binding sites	472:489	For example, hydrogel-based biomaterials with cell-binding sites, tunable mechanical properties, and complex architectures have emerged as a powerful tool to control cell adhesion and proliferation for tissue engineering.
32539334	6	76	theme	parameters	1085:1094	arg1	analysis					1070:1077	the principal component analysis	1046:1077	the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior	1046:1185	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	0	77	theme	Functionalized	79:92	arg1	Materials					114:122	Particle Functionalized Composites Hydrogel Materials	70:122	Particle Functionalized Composites Hydrogel Materials	70:122	Identification and Analysis of Key Parameters for the Ossification on Particle Functionalized Composites Hydrogel Materials.
32539334	6	78	theme	component	1060:1068	arg1	analysis					1070:1077	the principal component analysis	1046:1077	the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior	1046:1185	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	0	79	theme	Hydrogel	105:112	arg1	Materials					114:122	Particle Functionalized Composites Hydrogel Materials	70:122	Particle Functionalized Composites Hydrogel Materials	70:122	Identification and Analysis of Key Parameters for the Ossification on Particle Functionalized Composites Hydrogel Materials.
32539334	6	80	dep	parameters	1085:1094	arg1	size					1153:1156	particle size	1144:1156	particle size	1144:1156	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	80	dep	parameters	1085:1094	arg1	mass					1163:1166	mass	1163:1166	mass	1163:1166	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	80	dep	parameters	1085:1094	arg1	parameters					1085:1094	18 parameters	1082:1094	18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass)	1082:1167	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	80	dep	parameters	1085:1094	arg1	modulus					1105:1111	Young's modulus	1097:1111	Young's modulus of the hydrogel and particles	1097:1141	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	3	81	theme	hydrogel-based	439:452	arg1	biomaterials					454:465	hydrogel-based biomaterials	439:465	hydrogel-based biomaterials with cell-binding sites, tunable mechanical properties, and complex architectures	439:547	For example, hydrogel-based biomaterials with cell-binding sites, tunable mechanical properties, and complex architectures have emerged as a powerful tool to control cell adhesion and proliferation for tissue engineering.
32539334	5	82	theme	annealing	846:854	arg1	approach					856:863	a thermal annealing approach	836:863	a thermal annealing approach	836:863	In current work, we have designed new osteoinductive gellan gum hydrogels by a thermal annealing approach and consequently functionalized them with Ca/Mg carbonate submicron particles.
32539334	7	83	from	modulus	1542:1548	arg1	hydrogel					1555:1562	a hydrogel	1553:1562	a hydrogel	1553:1562	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	8	84	theme	efficient	1817:1825	arg1	optimization					1827:1838	efficient optimization	1817:1838	efficient optimization of bone reconstruction and other cell growth applications	1817:1896	The detailed analysis presented here allows identifying hydrogels for cell growth applications, while on the other hand, material properties can be predicted, and their overall number can be minimized leading to efficient optimization of bone reconstruction and other cell growth applications.
32539334	3	85	theme	tunable	492:498	arg1	properties					511:520	tunable mechanical properties	492:520	tunable mechanical properties	492:520	For example, hydrogel-based biomaterials with cell-binding sites, tunable mechanical properties, and complex architectures have emerged as a powerful tool to control cell adhesion and proliferation for tissue engineering.
32539334	5	86	theme	gellan	812:817	arg1	hydrogels					823:831	new osteoinductive gellan gum hydrogels	793:831	new osteoinductive gellan gum hydrogels	793:831	In current work, we have designed new osteoinductive gellan gum hydrogels by a thermal annealing approach and consequently functionalized them with Ca/Mg carbonate submicron particles.
32539334	6	87	theme	Young	1097:1101	arg1	modulus					1105:1111	Young's modulus	1097:1111	Young's modulus of the hydrogel and particles	1097:1141	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	6	87	theme	Young	1097:1101	arg1	parameters					1085:1094	18 parameters	1082:1094	18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass)	1082:1167	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32539334	2	88	theme	techniques	414:423	arg1	number					395:400	the number	391:400	the number of advanced techniques	391:423	The major challenge is the labor-intensive data mining as well as requirements of the number of advanced techniques.
32539334	7	89	theme	high	1529:1532	arg1	modulus					1542:1548	a high Young's modulus	1527:1548	a high Young's modulus in a hydrogel	1527:1562	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	2	90	theme	labor-intensive	336:350	arg1	challenge					319:327	The major challenge	309:327	The major challenge	309:327	The major challenge is the labor-intensive data mining as well as requirements of the number of advanced techniques.
32539334	2	90	theme	labor-intensive	336:350	arg1	data					352:355	the labor-intensive data	332:355	the labor-intensive data mining as well as requirements of the number of advanced techniques	332:423	The major challenge is the labor-intensive data mining as well as requirements of the number of advanced techniques.
32539334	7	91	theme	local	1510:1514	arg1	areas					1516:1520	providing local areas	1500:1520	providing local areas with a high Young's modulus in a hydrogel	1500:1562	It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young's modulus in a hydrogel, a desirable property for cell adhesion.
32539334	8	92	theme	cell	1675:1678	arg1	applications					1687:1698	cell growth applications	1675:1698	cell growth applications	1675:1698	The detailed analysis presented here allows identifying hydrogels for cell growth applications, while on the other hand, material properties can be predicted, and their overall number can be minimized leading to efficient optimization of bone reconstruction and other cell growth applications.
32539334	6	93	theme	successful	995:1004	arg1	generation					1021:1030	a successful hydroxyapatite generation	993:1030	a successful hydroxyapatite generation	993:1030	Determination of key parameters, which influence a successful hydroxyapatite generation, was done via the principal component analysis of 18 parameters (Young's modulus of the hydrogel and particles, particle size, and mass) and cell behavior at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production, and cells area) obtained by characterizing such composite hydrogel.
32315005	4	0	theme	improved	769:776	arg1	metabolism					784:793	improved lipid metabolism	769:793	improved lipid metabolism	769:793	The results showed that NFP reduced body weight gain and improved lipid metabolism, hepatic oxidative stress, and inflammation in rats under a HFD.
32315005	8	1	theme	gut	1529:1531	arg1	microflora					1533:1542	the gut microflora	1525:1542	the gut microflora	1525:1542	Thus, NFP may modulate the gut microflora and SCFA production and reduce the permeability of the colonic barrier and metabolic endotoxemia, thereby alleviating hepatic oxidative stress and inflammation in rats under a HFD.
32315005	2	2	theme	fatty	459:463	arg1	SCFAs					472:476	SCFAs	472:476	SCFAs	472:476	We aimed to investigate the possible mechanism by which noni fruit polysaccharide (NFP) improved hepatic oxidative stress and inflammation in rats under a high-fat diet (HFD) by modulating short-chain fatty acids (SCFAs), the intestinal barrier, and gut microbiota.
32315005	2	2	theme	fatty	459:463	arg1	acids					465:469	short-chain fatty acids	447:469	short-chain fatty acids (SCFAs)	447:477	We aimed to investigate the possible mechanism by which noni fruit polysaccharide (NFP) improved hepatic oxidative stress and inflammation in rats under a high-fat diet (HFD) by modulating short-chain fatty acids (SCFAs), the intestinal barrier, and gut microbiota.
32315005	0	3	from	effects	12:18	arg1	inflammation					81:92	inflammation	81:92	inflammation	81:92	Alleviating effects of noni fruit polysaccharide on hepatic oxidative stress and inflammation in rats under a high-fat diet and its possible mechanisms.
32315005	0	3	from	effects	12:18	arg1	stress					70:75	hepatic oxidative stress	52:75	hepatic oxidative stress	52:75	Alleviating effects of noni fruit polysaccharide on hepatic oxidative stress and inflammation in rats under a high-fat diet and its possible mechanisms.
32315005	6	4	dep	_coprostanoligenes_group	1130:1153	arg1	[Eubacterium					1117:1128	Parasutterella, [Eubacterium]_coprostanoligenes_group	1101:1153	[Eubacterium	1117:1128	The levels of Lactobacillus, Ruminococcaceae_UCG_014, Parasutterella, [Eubacterium]_coprostanoligenes_group, and Ruminococcus_1 improved, whereas the levels of Prevotella_9, Collinsella, Bacteroides, and Turicibacter decreased.
32315005	1	5	theme	Non-alcoholic	153:165	arg1	disease					179:185	Non-alcoholic fatty liver disease	153:185	Non-alcoholic fatty liver disease	153:185	Non-alcoholic fatty liver disease is associated with gut microbiota, oxidative stress, and inflammation.
32315005	5	6	theme	HFD-induced	958:968	arg1	dysbiosis					974:982	the HFD-induced gut dysbiosis	954:982	the HFD-induced gut dysbiosis	954:982	Aside from these beneficial effects, NFP positively affected the SCFA production and reversed the HFD-induced gut dysbiosis as indicated by improved microbiota diversity and composition.
32315005	2	7	theme	intestinal	484:493	arg1	barrier					495:501	the intestinal barrier	480:501	the intestinal barrier	480:501	We aimed to investigate the possible mechanism by which noni fruit polysaccharide (NFP) improved hepatic oxidative stress and inflammation in rats under a high-fat diet (HFD) by modulating short-chain fatty acids (SCFAs), the intestinal barrier, and gut microbiota.
32315005	6	8	theme	Parasutterella	1101:1114	arg1	_coprostanoligenes_group					1130:1153	Parasutterella, [Eubacterium]_coprostanoligenes_group	1101:1153	_coprostanoligenes_group	1130:1153	The levels of Lactobacillus, Ruminococcaceae_UCG_014, Parasutterella, [Eubacterium]_coprostanoligenes_group, and Ruminococcus_1 improved, whereas the levels of Prevotella_9, Collinsella, Bacteroides, and Turicibacter decreased.
32315005	8	9	from	stress	1680:1685	arg1	rats					1707:1710	rats	1707:1710	rats	1707:1710	Thus, NFP may modulate the gut microflora and SCFA production and reduce the permeability of the colonic barrier and metabolic endotoxemia, thereby alleviating hepatic oxidative stress and inflammation in rats under a HFD.
32315005	7	10	theme	mRNA	1348:1351	arg1	expression					1362:1371	the mRNA relative expression	1344:1371	the mRNA relative expression of CCL5, ZO-1, and occludin in the colon	1344:1412	Furthermore, NFP maintained the colonic barrier integrity (increased the mRNA relative expression of CCL5, ZO-1, and occludin in the colon, and decreased the serum CCL5 level), and decreased the serum lipopolysaccharide level.
32315005	4	11	from	gain	760:763	arg1	rats					842:845	rats	842:845	rats	842:845	The results showed that NFP reduced body weight gain and improved lipid metabolism, hepatic oxidative stress, and inflammation in rats under a HFD.
32315005	4	12	theme	lipid	778:782	arg1	metabolism					784:793	improved lipid metabolism	769:793	improved lipid metabolism	769:793	The results showed that NFP reduced body weight gain and improved lipid metabolism, hepatic oxidative stress, and inflammation in rats under a HFD.
32315005	8	13	theme	barrier	1607:1613	arg1	permeability					1579:1590	the permeability	1575:1590	the permeability of the colonic barrier	1575:1613	Thus, NFP may modulate the gut microflora and SCFA production and reduce the permeability of the colonic barrier and metabolic endotoxemia, thereby alleviating hepatic oxidative stress and inflammation in rats under a HFD.
32315005	8	13	theme	barrier	1607:1613	arg1	endotoxemia					1629:1639	metabolic endotoxemia	1619:1639	metabolic endotoxemia	1619:1639	Thus, NFP may modulate the gut microflora and SCFA production and reduce the permeability of the colonic barrier and metabolic endotoxemia, thereby alleviating hepatic oxidative stress and inflammation in rats under a HFD.
32315005	7	14	theme	occludin	1392:1399	arg1	expression					1362:1371	the mRNA relative expression	1344:1371	the mRNA relative expression of CCL5, ZO-1, and occludin in the colon	1344:1412	Furthermore, NFP maintained the colonic barrier integrity (increased the mRNA relative expression of CCL5, ZO-1, and occludin in the colon, and decreased the serum CCL5 level), and decreased the serum lipopolysaccharide level.
32315005	4	15	theme	body	748:751	arg1	gain					760:763	body weight gain	748:763	body weight gain	748:763	The results showed that NFP reduced body weight gain and improved lipid metabolism, hepatic oxidative stress, and inflammation in rats under a HFD.
32315005	6	16	theme	Ruminococcaceae_UCG_014	1076:1098	arg1	levels					1051:1056	The levels	1047:1056	The levels of Lactobacillus, Ruminococcaceae_UCG_014, Parasutterella, [Eubacterium]_coprostanoligenes_group, and Ruminococcus_1	1047:1173	The levels of Lactobacillus, Ruminococcaceae_UCG_014, Parasutterella, [Eubacterium]_coprostanoligenes_group, and Ruminococcus_1 improved, whereas the levels of Prevotella_9, Collinsella, Bacteroides, and Turicibacter decreased.
32315005	8	17	theme	colonic	1599:1605	arg1	barrier					1607:1613	the colonic barrier	1595:1613	the colonic barrier	1595:1613	Thus, NFP may modulate the gut microflora and SCFA production and reduce the permeability of the colonic barrier and metabolic endotoxemia, thereby alleviating hepatic oxidative stress and inflammation in rats under a HFD.
32315005	1	18	theme	fatty	167:171	arg1	disease					179:185	Non-alcoholic fatty liver disease	153:185	Non-alcoholic fatty liver disease	153:185	Non-alcoholic fatty liver disease is associated with gut microbiota, oxidative stress, and inflammation.
32315005	5	19	theme	improved	1000:1007	arg1	diversity					1020:1028	improved microbiota diversity	1000:1028	improved microbiota diversity	1000:1028	Aside from these beneficial effects, NFP positively affected the SCFA production and reversed the HFD-induced gut dysbiosis as indicated by improved microbiota diversity and composition.
32315005	0	20	theme	polysaccharide	34:47	arg1	effects					12:18	effects	12:18	effects of noni fruit polysaccharide on hepatic oxidative stress and inflammation in rats	12:100	Alleviating effects of noni fruit polysaccharide on hepatic oxidative stress and inflammation in rats under a high-fat diet and its possible mechanisms.
32315005	3	21	from	dysbiosis	572:580	arg1	rats					585:588	rats	585:588	rats	585:588	Hepatic oxidative stress, inflammation, and gut dysbiosis in rats were induced through HFD feeding for 4 weeks, followed by intervention with NFP treatment (100 mg per kg bw) for 5 weeks.
32315005	1	22	theme	liver	173:177	arg1	disease					179:185	Non-alcoholic fatty liver disease	153:185	Non-alcoholic fatty liver disease	153:185	Non-alcoholic fatty liver disease is associated with gut microbiota, oxidative stress, and inflammation.
32315005	5	23	theme	microbiota	1009:1018	arg1	diversity					1020:1028	improved microbiota diversity	1000:1028	improved microbiota diversity	1000:1028	Aside from these beneficial effects, NFP positively affected the SCFA production and reversed the HFD-induced gut dysbiosis as indicated by improved microbiota diversity and composition.
32315005	3	24	theme	Hepatic	524:530	arg1	stress					542:547	Hepatic oxidative stress	524:547	Hepatic oxidative stress	524:547	Hepatic oxidative stress, inflammation, and gut dysbiosis in rats were induced through HFD feeding for 4 weeks, followed by intervention with NFP treatment (100 mg per kg bw) for 5 weeks.
32315005	7	25	dep	integrity	1323:1331	arg1	increased					1334:1342	increased	1334:1342	increased the mRNA relative expression of CCL5, ZO-1, and occludin in the colon	1334:1412	Furthermore, NFP maintained the colonic barrier integrity (increased the mRNA relative expression of CCL5, ZO-1, and occludin in the colon, and decreased the serum CCL5 level), and decreased the serum lipopolysaccharide level.
32315005	7	25	dep	integrity	1323:1331	arg1	decreased					1419:1427	decreased	1419:1427	decreased the serum CCL5 level	1419:1448	Furthermore, NFP maintained the colonic barrier integrity (increased the mRNA relative expression of CCL5, ZO-1, and occludin in the colon, and decreased the serum CCL5 level), and decreased the serum lipopolysaccharide level.
32315005	4	26	theme	weight	753:758	arg1	gain					760:763	body weight gain	748:763	body weight gain	748:763	The results showed that NFP reduced body weight gain and improved lipid metabolism, hepatic oxidative stress, and inflammation in rats under a HFD.
32315005	2	27	dep	noni	314:317	arg1	polysaccharide					325:338	fruit polysaccharide	319:338	noni fruit polysaccharide (NFP)	314:344	We aimed to investigate the possible mechanism by which noni fruit polysaccharide (NFP) improved hepatic oxidative stress and inflammation in rats under a high-fat diet (HFD) by modulating short-chain fatty acids (SCFAs), the intestinal barrier, and gut microbiota.
32315005	2	27	dep	noni	314:317	arg1	NFP					341:343	NFP	341:343	NFP	341:343	We aimed to investigate the possible mechanism by which noni fruit polysaccharide (NFP) improved hepatic oxidative stress and inflammation in rats under a high-fat diet (HFD) by modulating short-chain fatty acids (SCFAs), the intestinal barrier, and gut microbiota.
32315005	3	28	from	stress	542:547	arg1	rats					585:588	rats	585:588	rats	585:588	Hepatic oxidative stress, inflammation, and gut dysbiosis in rats were induced through HFD feeding for 4 weeks, followed by intervention with NFP treatment (100 mg per kg bw) for 5 weeks.
32315005	2	29	theme	oxidative	363:371	arg1	stress					373:378	hepatic oxidative stress	355:378	hepatic oxidative stress	355:378	We aimed to investigate the possible mechanism by which noni fruit polysaccharide (NFP) improved hepatic oxidative stress and inflammation in rats under a high-fat diet (HFD) by modulating short-chain fatty acids (SCFAs), the intestinal barrier, and gut microbiota.
32315005	0	30	theme	noni	23:26	arg1	polysaccharide					34:47	noni fruit polysaccharide	23:47	noni fruit polysaccharide	23:47	Alleviating effects of noni fruit polysaccharide on hepatic oxidative stress and inflammation in rats under a high-fat diet and its possible mechanisms.
32315005	7	31	theme	relative	1353:1360	arg1	expression					1362:1371	the mRNA relative expression	1344:1371	the mRNA relative expression of CCL5, ZO-1, and occludin in the colon	1344:1412	Furthermore, NFP maintained the colonic barrier integrity (increased the mRNA relative expression of CCL5, ZO-1, and occludin in the colon, and decreased the serum CCL5 level), and decreased the serum lipopolysaccharide level.
32315005	2	32	theme	gut	508:510	arg1	microbiota					512:521	gut microbiota	508:521	gut microbiota	508:521	We aimed to investigate the possible mechanism by which noni fruit polysaccharide (NFP) improved hepatic oxidative stress and inflammation in rats under a high-fat diet (HFD) by modulating short-chain fatty acids (SCFAs), the intestinal barrier, and gut microbiota.
32315005	7	33	theme	barrier	1315:1321	arg1	integrity					1323:1331	the colonic barrier integrity	1303:1331	the colonic barrier integrity (increased the mRNA relative expression of CCL5, ZO-1, and occludin in the colon, and decreased the serum CCL5 level)	1303:1449	Furthermore, NFP maintained the colonic barrier integrity (increased the mRNA relative expression of CCL5, ZO-1, and occludin in the colon, and decreased the serum CCL5 level), and decreased the serum lipopolysaccharide level.
32315005	2	34	theme	high-fat	413:420	arg1	HFD					428:430	HFD	428:430	HFD	428:430	We aimed to investigate the possible mechanism by which noni fruit polysaccharide (NFP) improved hepatic oxidative stress and inflammation in rats under a high-fat diet (HFD) by modulating short-chain fatty acids (SCFAs), the intestinal barrier, and gut microbiota.
32315005	2	34	theme	high-fat	413:420	arg1	diet					422:425	a high-fat diet	411:425	a high-fat diet (HFD)	411:431	We aimed to investigate the possible mechanism by which noni fruit polysaccharide (NFP) improved hepatic oxidative stress and inflammation in rats under a high-fat diet (HFD) by modulating short-chain fatty acids (SCFAs), the intestinal barrier, and gut microbiota.
32315005	7	35	theme	serum	1470:1474	arg1	level					1495:1499	the serum lipopolysaccharide level	1466:1499	the serum lipopolysaccharide level	1466:1499	Furthermore, NFP maintained the colonic barrier integrity (increased the mRNA relative expression of CCL5, ZO-1, and occludin in the colon, and decreased the serum CCL5 level), and decreased the serum lipopolysaccharide level.
32315005	0	36	theme	high-fat	110:117	arg1	diet					119:122	a high-fat diet	108:122	a high-fat diet	108:122	Alleviating effects of noni fruit polysaccharide on hepatic oxidative stress and inflammation in rats under a high-fat diet and its possible mechanisms.
32315005	0	37	from	inflammation	81:92	arg1	rats					97:100	rats	97:100	rats	97:100	Alleviating effects of noni fruit polysaccharide on hepatic oxidative stress and inflammation in rats under a high-fat diet and its possible mechanisms.
32315005	2	38	theme	possible	286:293	arg1	mechanism					295:303	the possible mechanism	282:303	the possible mechanism by which noni fruit polysaccharide (NFP) improved hepatic oxidative stress and inflammation in rats under a high-fat diet (HFD) by modulating short-chain fatty acids (SCFAs), the intestinal barrier, and gut microbiota	282:521	We aimed to investigate the possible mechanism by which noni fruit polysaccharide (NFP) improved hepatic oxidative stress and inflammation in rats under a high-fat diet (HFD) by modulating short-chain fatty acids (SCFAs), the intestinal barrier, and gut microbiota.
32315005	3	39	from	inflammation	550:561	arg1	rats					585:588	rats	585:588	rats	585:588	Hepatic oxidative stress, inflammation, and gut dysbiosis in rats were induced through HFD feeding for 4 weeks, followed by intervention with NFP treatment (100 mg per kg bw) for 5 weeks.
32315005	2	40	theme	short-chain	447:457	arg1	SCFAs					472:476	SCFAs	472:476	SCFAs	472:476	We aimed to investigate the possible mechanism by which noni fruit polysaccharide (NFP) improved hepatic oxidative stress and inflammation in rats under a high-fat diet (HFD) by modulating short-chain fatty acids (SCFAs), the intestinal barrier, and gut microbiota.
32315005	2	40	theme	short-chain	447:457	arg1	acids					465:469	short-chain fatty acids	447:469	short-chain fatty acids (SCFAs)	447:477	We aimed to investigate the possible mechanism by which noni fruit polysaccharide (NFP) improved hepatic oxidative stress and inflammation in rats under a high-fat diet (HFD) by modulating short-chain fatty acids (SCFAs), the intestinal barrier, and gut microbiota.
32315005	8	41	theme	metabolic	1619:1627	arg1	endotoxemia					1629:1639	metabolic endotoxemia	1619:1639	metabolic endotoxemia	1619:1639	Thus, NFP may modulate the gut microflora and SCFA production and reduce the permeability of the colonic barrier and metabolic endotoxemia, thereby alleviating hepatic oxidative stress and inflammation in rats under a HFD.
32315005	4	42	from	inflammation	826:837	arg1	rats					842:845	rats	842:845	rats	842:845	The results showed that NFP reduced body weight gain and improved lipid metabolism, hepatic oxidative stress, and inflammation in rats under a HFD.
32315005	6	43	theme	Collinsella	1221:1231	arg1	levels					1197:1202	the levels	1193:1202	the levels of Prevotella_9, Collinsella, Bacteroides, and Turicibacter	1193:1262	The levels of Lactobacillus, Ruminococcaceae_UCG_014, Parasutterella, [Eubacterium]_coprostanoligenes_group, and Ruminococcus_1 improved, whereas the levels of Prevotella_9, Collinsella, Bacteroides, and Turicibacter decreased.
32315005	7	44	theme	serum	1433:1437	arg1	level					1444:1448	the serum CCL5 level	1429:1448	the serum CCL5 level	1429:1448	Furthermore, NFP maintained the colonic barrier integrity (increased the mRNA relative expression of CCL5, ZO-1, and occludin in the colon, and decreased the serum CCL5 level), and decreased the serum lipopolysaccharide level.
32315005	1	45	theme	gut	206:208	arg1	microbiota					210:219	gut microbiota	206:219	gut microbiota	206:219	Non-alcoholic fatty liver disease is associated with gut microbiota, oxidative stress, and inflammation.
32315005	0	46	theme	fruit	28:32	arg1	polysaccharide					34:47	noni fruit polysaccharide	23:47	noni fruit polysaccharide	23:47	Alleviating effects of noni fruit polysaccharide on hepatic oxidative stress and inflammation in rats under a high-fat diet and its possible mechanisms.
32315005	6	47	theme	Turicibacter	1251:1262	arg1	levels					1197:1202	the levels	1193:1202	the levels of Prevotella_9, Collinsella, Bacteroides, and Turicibacter	1193:1262	The levels of Lactobacillus, Ruminococcaceae_UCG_014, Parasutterella, [Eubacterium]_coprostanoligenes_group, and Ruminococcus_1 improved, whereas the levels of Prevotella_9, Collinsella, Bacteroides, and Turicibacter decreased.
32315005	0	48	from	stress	70:75	arg1	rats					97:100	rats	97:100	rats	97:100	Alleviating effects of noni fruit polysaccharide on hepatic oxidative stress and inflammation in rats under a high-fat diet and its possible mechanisms.
32315005	6	49	theme	Ruminococcus_1	1160:1173	arg1	levels					1051:1056	The levels	1047:1056	The levels of Lactobacillus, Ruminococcaceae_UCG_014, Parasutterella, [Eubacterium]_coprostanoligenes_group, and Ruminococcus_1	1047:1173	The levels of Lactobacillus, Ruminococcaceae_UCG_014, Parasutterella, [Eubacterium]_coprostanoligenes_group, and Ruminococcus_1 improved, whereas the levels of Prevotella_9, Collinsella, Bacteroides, and Turicibacter decreased.
32315005	2	50	from	stress	373:378	arg1	rats					400:403	rats	400:403	rats	400:403	We aimed to investigate the possible mechanism by which noni fruit polysaccharide (NFP) improved hepatic oxidative stress and inflammation in rats under a high-fat diet (HFD) by modulating short-chain fatty acids (SCFAs), the intestinal barrier, and gut microbiota.
32315005	8	51	from	inflammation	1691:1702	arg1	rats					1707:1710	rats	1707:1710	rats	1707:1710	Thus, NFP may modulate the gut microflora and SCFA production and reduce the permeability of the colonic barrier and metabolic endotoxemia, thereby alleviating hepatic oxidative stress and inflammation in rats under a HFD.
32315005	0	52	theme	hepatic	52:58	arg1	stress					70:75	hepatic oxidative stress	52:75	hepatic oxidative stress	52:75	Alleviating effects of noni fruit polysaccharide on hepatic oxidative stress and inflammation in rats under a high-fat diet and its possible mechanisms.
32315005	4	53	theme	hepatic	796:802	arg1	stress					814:819	hepatic oxidative stress	796:819	hepatic oxidative stress	796:819	The results showed that NFP reduced body weight gain and improved lipid metabolism, hepatic oxidative stress, and inflammation in rats under a HFD.
32315005	7	54	theme	colonic	1307:1313	arg1	integrity					1323:1331	the colonic barrier integrity	1303:1331	the colonic barrier integrity (increased the mRNA relative expression of CCL5, ZO-1, and occludin in the colon, and decreased the serum CCL5 level)	1303:1449	Furthermore, NFP maintained the colonic barrier integrity (increased the mRNA relative expression of CCL5, ZO-1, and occludin in the colon, and decreased the serum CCL5 level), and decreased the serum lipopolysaccharide level.
32315005	7	55	theme	ZO-1	1382:1385	arg1	expression					1362:1371	the mRNA relative expression	1344:1371	the mRNA relative expression of CCL5, ZO-1, and occludin in the colon	1344:1412	Furthermore, NFP maintained the colonic barrier integrity (increased the mRNA relative expression of CCL5, ZO-1, and occludin in the colon, and decreased the serum CCL5 level), and decreased the serum lipopolysaccharide level.
32315005	3	56	dep	NFP	666:668	arg1	treatment					670:678	treatment	670:678	NFP treatment (100 mg per kg bw)	666:697	Hepatic oxidative stress, inflammation, and gut dysbiosis in rats were induced through HFD feeding for 4 weeks, followed by intervention with NFP treatment (100 mg per kg bw) for 5 weeks.
32315005	0	57	theme	possible	132:139	arg1	mechanisms					141:150	its possible mechanisms	128:150	its possible mechanisms	128:150	Alleviating effects of noni fruit polysaccharide on hepatic oxidative stress and inflammation in rats under a high-fat diet and its possible mechanisms.
32315005	4	58	from	stress	814:819	arg1	rats					842:845	rats	842:845	rats	842:845	The results showed that NFP reduced body weight gain and improved lipid metabolism, hepatic oxidative stress, and inflammation in rats under a HFD.
32315005	5	59	theme	SCFA	925:928	arg1	production					930:939	the SCFA production	921:939	the SCFA production	921:939	Aside from these beneficial effects, NFP positively affected the SCFA production and reversed the HFD-induced gut dysbiosis as indicated by improved microbiota diversity and composition.
32315005	2	60	from	inflammation	384:395	arg1	rats					400:403	rats	400:403	rats	400:403	We aimed to investigate the possible mechanism by which noni fruit polysaccharide (NFP) improved hepatic oxidative stress and inflammation in rats under a high-fat diet (HFD) by modulating short-chain fatty acids (SCFAs), the intestinal barrier, and gut microbiota.
32315005	7	61	theme	CCL5	1439:1442	arg1	level					1444:1448	the serum CCL5 level	1429:1448	the serum CCL5 level	1429:1448	Furthermore, NFP maintained the colonic barrier integrity (increased the mRNA relative expression of CCL5, ZO-1, and occludin in the colon, and decreased the serum CCL5 level), and decreased the serum lipopolysaccharide level.
32315005	3	62	with	intervention	648:659	arg1	NFP					666:668	NFP treatment (100 mg per kg bw)	666:697	NFP treatment (100 mg per kg bw)	666:697	Hepatic oxidative stress, inflammation, and gut dysbiosis in rats were induced through HFD feeding for 4 weeks, followed by intervention with NFP treatment (100 mg per kg bw) for 5 weeks.
32315005	3	63	dep	treatment	670:678	arg1	mg					685:686	100 mg	681:686	100 mg per kg bw	681:696	Hepatic oxidative stress, inflammation, and gut dysbiosis in rats were induced through HFD feeding for 4 weeks, followed by intervention with NFP treatment (100 mg per kg bw) for 5 weeks.
32315005	6	64	theme	Bacteroides	1234:1244	arg1	levels					1197:1202	the levels	1193:1202	the levels of Prevotella_9, Collinsella, Bacteroides, and Turicibacter	1193:1262	The levels of Lactobacillus, Ruminococcaceae_UCG_014, Parasutterella, [Eubacterium]_coprostanoligenes_group, and Ruminococcus_1 improved, whereas the levels of Prevotella_9, Collinsella, Bacteroides, and Turicibacter decreased.
32315005	6	65	theme	_coprostanoligenes_group	1130:1153	arg1	levels					1051:1056	The levels	1047:1056	The levels of Lactobacillus, Ruminococcaceae_UCG_014, Parasutterella, [Eubacterium]_coprostanoligenes_group, and Ruminococcus_1	1047:1173	The levels of Lactobacillus, Ruminococcaceae_UCG_014, Parasutterella, [Eubacterium]_coprostanoligenes_group, and Ruminococcus_1 improved, whereas the levels of Prevotella_9, Collinsella, Bacteroides, and Turicibacter decreased.
32315005	5	66	theme	beneficial	877:886	arg1	effects					888:894	these beneficial effects	871:894	these beneficial effects	871:894	Aside from these beneficial effects, NFP positively affected the SCFA production and reversed the HFD-induced gut dysbiosis as indicated by improved microbiota diversity and composition.
32315005	8	67	theme	oxidative	1670:1678	arg1	stress					1680:1685	hepatic oxidative stress	1662:1685	hepatic oxidative stress	1662:1685	Thus, NFP may modulate the gut microflora and SCFA production and reduce the permeability of the colonic barrier and metabolic endotoxemia, thereby alleviating hepatic oxidative stress and inflammation in rats under a HFD.
32315005	2	68	theme	fruit	319:323	arg1	polysaccharide					325:338	fruit polysaccharide	319:338	noni fruit polysaccharide (NFP)	314:344	We aimed to investigate the possible mechanism by which noni fruit polysaccharide (NFP) improved hepatic oxidative stress and inflammation in rats under a high-fat diet (HFD) by modulating short-chain fatty acids (SCFAs), the intestinal barrier, and gut microbiota.
32315005	2	68	theme	fruit	319:323	arg1	NFP					341:343	NFP	341:343	NFP	341:343	We aimed to investigate the possible mechanism by which noni fruit polysaccharide (NFP) improved hepatic oxidative stress and inflammation in rats under a high-fat diet (HFD) by modulating short-chain fatty acids (SCFAs), the intestinal barrier, and gut microbiota.
32315005	8	69	theme	SCFA	1548:1551	arg1	production					1553:1562	SCFA production	1548:1562	SCFA production	1548:1562	Thus, NFP may modulate the gut microflora and SCFA production and reduce the permeability of the colonic barrier and metabolic endotoxemia, thereby alleviating hepatic oxidative stress and inflammation in rats under a HFD.
32315005	4	70	theme	oxidative	804:812	arg1	stress					814:819	hepatic oxidative stress	796:819	hepatic oxidative stress	796:819	The results showed that NFP reduced body weight gain and improved lipid metabolism, hepatic oxidative stress, and inflammation in rats under a HFD.
32315005	3	71	theme	HFD	611:613	arg1	feeding					615:621	HFD feeding	611:621	HFD feeding	611:621	Hepatic oxidative stress, inflammation, and gut dysbiosis in rats were induced through HFD feeding for 4 weeks, followed by intervention with NFP treatment (100 mg per kg bw) for 5 weeks.
32315005	7	72	theme	CCL5	1376:1379	arg1	expression					1362:1371	the mRNA relative expression	1344:1371	the mRNA relative expression of CCL5, ZO-1, and occludin in the colon	1344:1412	Furthermore, NFP maintained the colonic barrier integrity (increased the mRNA relative expression of CCL5, ZO-1, and occludin in the colon, and decreased the serum CCL5 level), and decreased the serum lipopolysaccharide level.
32315005	5	73	theme	gut	970:972	arg1	dysbiosis					974:982	the HFD-induced gut dysbiosis	954:982	the HFD-induced gut dysbiosis	954:982	Aside from these beneficial effects, NFP positively affected the SCFA production and reversed the HFD-induced gut dysbiosis as indicated by improved microbiota diversity and composition.
32315005	1	74	theme	oxidative	222:230	arg1	stress					232:237	oxidative stress	222:237	oxidative stress	222:237	Non-alcoholic fatty liver disease is associated with gut microbiota, oxidative stress, and inflammation.
32315005	2	75	theme	hepatic	355:361	arg1	stress					373:378	hepatic oxidative stress	355:378	hepatic oxidative stress	355:378	We aimed to investigate the possible mechanism by which noni fruit polysaccharide (NFP) improved hepatic oxidative stress and inflammation in rats under a high-fat diet (HFD) by modulating short-chain fatty acids (SCFAs), the intestinal barrier, and gut microbiota.
32315005	8	76	theme	hepatic	1662:1668	arg1	stress					1680:1685	hepatic oxidative stress	1662:1685	hepatic oxidative stress	1662:1685	Thus, NFP may modulate the gut microflora and SCFA production and reduce the permeability of the colonic barrier and metabolic endotoxemia, thereby alleviating hepatic oxidative stress and inflammation in rats under a HFD.
32315005	7	77	theme	lipopolysaccharide	1476:1493	arg1	level					1495:1499	the serum lipopolysaccharide level	1466:1499	the serum lipopolysaccharide level	1466:1499	Furthermore, NFP maintained the colonic barrier integrity (increased the mRNA relative expression of CCL5, ZO-1, and occludin in the colon, and decreased the serum CCL5 level), and decreased the serum lipopolysaccharide level.
32315005	0	78	theme	oxidative	60:68	arg1	stress					70:75	hepatic oxidative stress	52:75	hepatic oxidative stress	52:75	Alleviating effects of noni fruit polysaccharide on hepatic oxidative stress and inflammation in rats under a high-fat diet and its possible mechanisms.
32315005	4	79	from	metabolism	784:793	arg1	rats					842:845	rats	842:845	rats	842:845	The results showed that NFP reduced body weight gain and improved lipid metabolism, hepatic oxidative stress, and inflammation in rats under a HFD.
32315005	3	80	theme	gut	568:570	arg1	dysbiosis					572:580	gut dysbiosis	568:580	gut dysbiosis	568:580	Hepatic oxidative stress, inflammation, and gut dysbiosis in rats were induced through HFD feeding for 4 weeks, followed by intervention with NFP treatment (100 mg per kg bw) for 5 weeks.
32315005	3	81	theme	oxidative	532:540	arg1	stress					542:547	Hepatic oxidative stress	524:547	Hepatic oxidative stress	524:547	Hepatic oxidative stress, inflammation, and gut dysbiosis in rats were induced through HFD feeding for 4 weeks, followed by intervention with NFP treatment (100 mg per kg bw) for 5 weeks.
32315005	7	82	from	expression	1362:1371	arg1	colon					1408:1412	the colon	1404:1412	the colon	1404:1412	Furthermore, NFP maintained the colonic barrier integrity (increased the mRNA relative expression of CCL5, ZO-1, and occludin in the colon, and decreased the serum CCL5 level), and decreased the serum lipopolysaccharide level.
34342947	4	0	theme	20 μg/mL	950:957	arg1	concentration					933:945	concentration	933:945	concentration of 20 μg/mL	933:957	As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment.
34342947	4	1	dep	20	853:854	arg1	to					850:851	to	850:851	to	850:851	As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment.
34342947	6	2	theme	T. indica	1243:1251	arg1	TSP					1236:1238	TSP	1236:1238	TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases	1236:1401	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	6	3	from	engineering	1325:1335	arg1	TSP					1236:1238	TSP	1236:1238	TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases	1236:1401	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	3	4	theme	tamarind	644:651	arg1	polysaccharide					658:671	tamarind seed polysaccharide sulfate (TSPS)	644:686	tamarind seed polysaccharide sulfate (TSPS)	644:686	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data revealed that tamarind seed polysaccharide sulfate (TSPS) was successfully prepared.
34342947	6	5	theme	osteogenic	1199:1208	arg1	compounds					1210:1218	osteogenic compounds	1199:1218	osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases	1199:1401	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	6	6	theme	bone-related	1381:1392	arg1	diseases					1394:1401	other bone-related diseases	1375:1401	other bone-related diseases	1375:1401	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	6	7	theme	tissue	1318:1323	arg1	engineering					1325:1335	bone tissue engineering	1313:1335	bone tissue engineering	1313:1335	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	3	8	theme	seed	653:656	arg1	polysaccharide					658:671	tamarind seed polysaccharide sulfate (TSPS)	644:686	tamarind seed polysaccharide sulfate (TSPS)	644:686	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data revealed that tamarind seed polysaccharide sulfate (TSPS) was successfully prepared.
34342947	5	9	from	expression	1098:1107	arg1	formation					1141:1149	bone formation	1136:1149	bone formation	1136:1149	Furthermore, TSPS enhanced expression of several marker genes in bone formation.
34342947	0	10	theme	bone	220:223	arg1	formation					225:233	bone formation	220:233	bone formation	220:233	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	6	11	theme	bone	1313:1316	arg1	engineering					1325:1335	bone tissue engineering	1313:1335	bone tissue engineering	1313:1335	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	4	12	located	observed	1044:1051	arg1	treatment					1060:1068	TSP treatment	1056:1068	TSP treatment	1056:1068	As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment.
34342947	4	12	located	observed	1044:1051	arg2	increase					985:992	no statistically increase	968:992	no statistically increase for both ALP activity and bone mineralization	968:1038	As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment.
34342947	1	13	dep	effective	297:305	arg1	osteogenic					307:316	osteogenic	307:316	osteogenic	307:316	Therefore, finding the effective osteogenic compounds for the treatment of osteoporosis is a promising research approach.
34342947	0	14	theme	bone	200:203	arg1	resorption					205:214	bone resorption	200:214	bone resorption	200:214	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	2	15	theme	indica	431:436	arg1	L.					438:439	Tamarindus indica L.	420:439	Tamarindus indica L.	420:439	In our study, tamarind (Tamarindus indica L.) seed polysaccharide (TSP) extracted from tamarind seed was subjected to synthesize its sulfate derivatives.
34342947	2	15	theme	indica	431:436	arg1	polysaccharide					447:460	tamarind (Tamarindus indica L.) seed polysaccharide	410:460	tamarind (Tamarindus indica L.) seed polysaccharide (TSP) extracted from tamarind seed	410:495	In our study, tamarind (Tamarindus indica L.) seed polysaccharide (TSP) extracted from tamarind seed was subjected to synthesize its sulfate derivatives.
34342947	1	16	theme	osteoporosis	349:360	arg1	treatment					336:344	the treatment	332:344	the treatment of osteoporosis	332:360	Therefore, finding the effective osteogenic compounds for the treatment of osteoporosis is a promising research approach.
34342947	2	17	theme	seed	442:445	arg1	TSP					463:465	TSP	463:465	TSP	463:465	In our study, tamarind (Tamarindus indica L.) seed polysaccharide (TSP) extracted from tamarind seed was subjected to synthesize its sulfate derivatives.
34342947	2	17	theme	seed	442:445	arg1	L.					438:439	Tamarindus indica L.	420:439	Tamarindus indica L.	420:439	In our study, tamarind (Tamarindus indica L.) seed polysaccharide (TSP) extracted from tamarind seed was subjected to synthesize its sulfate derivatives.
34342947	2	17	theme	seed	442:445	arg1	polysaccharide					447:460	tamarind (Tamarindus indica L.) seed polysaccharide	410:460	tamarind (Tamarindus indica L.) seed polysaccharide (TSP) extracted from tamarind seed	410:495	In our study, tamarind (Tamarindus indica L.) seed polysaccharide (TSP) extracted from tamarind seed was subjected to synthesize its sulfate derivatives.
34342947	0	18	theme	major	242:246	arg1	impact					248:253	a major impact	240:253	a major impact on the population	240:271	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	4	19	dep	%	855:855	arg1	20					853:854	20	853:854	20	853:854	As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment.
34342947	6	20	theme	other	1375:1379	arg1	diseases					1394:1401	other bone-related diseases	1375:1401	other bone-related diseases	1375:1401	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	6	21	from	TSP	1236:1238	arg1	development					1297:1307	drug development	1292:1307	drug development	1292:1307	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	6	21	from	TSP	1236:1238	arg1	engineering					1325:1335	bone tissue engineering	1313:1335	bone tissue engineering	1313:1335	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	2	22	theme	Tamarindus	420:429	arg1	L.					438:439	Tamarindus indica L.	420:439	Tamarindus indica L.	420:439	In our study, tamarind (Tamarindus indica L.) seed polysaccharide (TSP) extracted from tamarind seed was subjected to synthesize its sulfate derivatives.
34342947	2	22	theme	Tamarindus	420:429	arg1	polysaccharide					447:460	tamarind (Tamarindus indica L.) seed polysaccharide	410:460	tamarind (Tamarindus indica L.) seed polysaccharide (TSP) extracted from tamarind seed	410:495	In our study, tamarind (Tamarindus indica L.) seed polysaccharide (TSP) extracted from tamarind seed was subjected to synthesize its sulfate derivatives.
34342947	5	23	theme	bone	1136:1139	arg1	formation					1141:1149	bone formation	1136:1149	bone formation	1136:1149	Furthermore, TSPS enhanced expression of several marker genes in bone formation.
34342947	6	24	from	fundamentals	1276:1287	arg1	development					1297:1307	drug development	1292:1307	drug development	1292:1307	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	6	24	from	fundamentals	1276:1287	arg1	engineering					1325:1335	bone tissue engineering	1313:1335	bone tissue engineering	1313:1335	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	5	25	theme	marker	1120:1125	arg1	genes					1127:1131	several marker genes	1112:1131	several marker genes	1112:1131	Furthermore, TSPS enhanced expression of several marker genes in bone formation.
34342947	6	26	theme	osteoporosis	1358:1369	arg1	treatment					1345:1353	the treatment	1341:1353	the treatment of osteoporosis and other bone-related diseases	1341:1401	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	0	27	theme	In	0:1	arg1	activities					20:29	In vitro osteogenic activities	0:29	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation,	0:234	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	5	28	theme	genes	1127:1131	arg1	expression					1098:1107	expression	1098:1107	expression of several marker genes in bone formation	1098:1149	Furthermore, TSPS enhanced expression of several marker genes in bone formation.
34342947	4	29	theme	phosphatase	820:830	arg1	activity					838:845	alkaline phosphatase (ALP) activity	811:845	alkaline phosphatase (ALP) activity up to 20%	811:855	As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment.
34342947	4	30	theme	alkaline	811:818	arg1	phosphatase					820:830	alkaline phosphatase	811:830	alkaline phosphatase (ALP) activity up to 20%	811:855	As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment.
34342947	4	30	theme	alkaline	811:818	arg1	ALP					833:835	ALP	833:835	ALP	833:835	As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment.
34342947	4	31	theme	TSP	1056:1058	arg1	treatment					1060:1068	TSP treatment	1056:1068	TSP treatment	1056:1068	As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment.
34342947	3	32	theme	elemental	606:614	arg1	analysis					616:623	elemental analysis	606:623	elemental analysis	606:623	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data revealed that tamarind seed polysaccharide sulfate (TSPS) was successfully prepared.
34342947	2	33	theme	tamarind	410:417	arg1	TSP					463:465	TSP	463:465	TSP	463:465	In our study, tamarind (Tamarindus indica L.) seed polysaccharide (TSP) extracted from tamarind seed was subjected to synthesize its sulfate derivatives.
34342947	2	33	theme	tamarind	410:417	arg1	L.					438:439	Tamarindus indica L.	420:439	Tamarindus indica L.	420:439	In our study, tamarind (Tamarindus indica L.) seed polysaccharide (TSP) extracted from tamarind seed was subjected to synthesize its sulfate derivatives.
34342947	2	33	theme	tamarind	410:417	arg1	polysaccharide					447:460	tamarind (Tamarindus indica L.) seed polysaccharide	410:460	tamarind (Tamarindus indica L.) seed polysaccharide (TSP) extracted from tamarind seed	410:495	In our study, tamarind (Tamarindus indica L.) seed polysaccharide (TSP) extracted from tamarind seed was subjected to synthesize its sulfate derivatives.
34342947	6	34	theme	drug	1292:1295	arg1	development					1297:1307	drug development	1292:1307	drug development	1292:1307	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	6	35	theme	obtained	1165:1172	arg1	data					1174:1177	the obtained data	1161:1177	the obtained data	1161:1177	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	4	36	theme	osteoblast	769:778	arg1	differentiation					780:794	osteoblast differentiation	769:794	osteoblast differentiation	769:794	As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment.
34342947	0	37	theme	osteogenic	9:18	arg1	activities					20:29	In vitro osteogenic activities	0:29	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation,	0:234	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	3	38	dep	polysaccharide	658:671	arg1	TSPS					682:685	TSPS	682:685	TSPS	682:685	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data revealed that tamarind seed polysaccharide sulfate (TSPS) was successfully prepared.
34342947	3	38	dep	polysaccharide	658:671	arg1	sulfate					673:679	sulfate	673:679	tamarind seed polysaccharide sulfate (TSPS)	644:686	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data revealed that tamarind seed polysaccharide sulfate (TSPS) was successfully prepared.
34342947	4	39	dep	%	911:911	arg1	58					909:910	58	909:910	58	909:910	As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment.
34342947	6	40	from	development	1297:1307	arg1	TSP					1236:1238	TSP	1236:1238	TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases	1236:1401	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	3	41	theme	monosaccharide	574:587	arg1	compositions					589:600	monosaccharide compositions	574:600	monosaccharide compositions	574:600	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data revealed that tamarind seed polysaccharide sulfate (TSPS) was successfully prepared.
34342947	0	42	theme	sulfated	34:41	arg1	polysaccharide					57:70	sulfated derivative of polysaccharide	34:70	sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis	34:119	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	0	42	theme	sulfated	34:41	arg1	health					153:158	the most serious public health concerns	129:167	the most serious public health concerns caused by an imbalance between bone resorption and bone formation	129:233	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	0	42	theme	sulfated	34:41	arg1	one					122:124	one	122:124	one	122:124	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	6	43	theme	fundamentals	1276:1287	arg1	TSP					1236:1238	TSP	1236:1238	TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases	1236:1401	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	1	44	theme	promising	367:375	arg1	approach					386:393	a promising research approach	365:393	a promising research approach	365:393	Therefore, finding the effective osteogenic compounds for the treatment of osteoporosis is a promising research approach.
34342947	3	45	theme	NMR	557:559	arg1	data					625:628	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data	550:628	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data	550:628	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data revealed that tamarind seed polysaccharide sulfate (TSPS) was successfully prepared.
34342947	0	46	contain	has	236:238	arg1	activities					20:29	In vitro osteogenic activities	0:29	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation,	0:234	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	0	46	contain	has	236:238	arg2	impact					248:253	a major impact	240:253	a major impact on the population	240:271	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	4	47	theme	bone	875:878	arg1	mineralization					880:893	bone mineralization	875:893	bone mineralization	875:893	As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment.
34342947	1	48	theme	research	377:384	arg1	approach					386:393	a promising research approach	365:393	a promising research approach	365:393	Therefore, finding the effective osteogenic compounds for the treatment of osteoporosis is a promising research approach.
34342947	6	49	theme	diseases	1394:1401	arg1	treatment					1345:1353	the treatment	1341:1353	the treatment of osteoporosis and other bone-related diseases	1341:1401	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	4	50	dep	10 days	863:869	arg1	%					911:911	%	911:911	%	911:911	As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment.
34342947	0	51	theme	of	54:55	arg1	polysaccharide					57:70	sulfated derivative of polysaccharide	34:70	sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis	34:119	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	0	51	theme	of	54:55	arg1	health					153:158	the most serious public health concerns	129:167	the most serious public health concerns caused by an imbalance between bone resorption and bone formation	129:233	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	0	51	theme	of	54:55	arg1	one					122:124	one	122:124	one	122:124	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	0	52	theme	public	146:151	arg1	health					153:158	the most serious public health concerns	129:167	the most serious public health concerns caused by an imbalance between bone resorption and bone formation	129:233	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	3	53	theme	compositions	589:600	arg1	data					625:628	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data	550:628	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data	550:628	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data revealed that tamarind seed polysaccharide sulfate (TSPS) was successfully prepared.
34342947	0	54	theme	derivative	43:52	arg1	polysaccharide					57:70	sulfated derivative of polysaccharide	34:70	sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis	34:119	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	0	54	theme	derivative	43:52	arg1	health					153:158	the most serious public health concerns	129:167	the most serious public health concerns caused by an imbalance between bone resorption and bone formation	129:233	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	0	54	theme	derivative	43:52	arg1	one					122:124	one	122:124	one	122:124	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	5	55	theme	several	1112:1118	arg1	genes					1127:1131	several marker genes	1112:1131	several marker genes	1112:1131	Furthermore, TSPS enhanced expression of several marker genes in bone formation.
34342947	0	56	theme	serious	138:144	arg1	health					153:158	the most serious public health concerns	129:167	the most serious public health concerns caused by an imbalance between bone resorption and bone formation	129:233	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	3	57	theme	1H	554:555	arg1	NMR					557:559	1H NMR	554:559	1H NMR	554:559	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data revealed that tamarind seed polysaccharide sulfate (TSPS) was successfully prepared.
34342947	0	58	dep	health	153:158	arg1	concerns					160:167	concerns	160:167	concerns	160:167	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	4	59	dep	activity	838:845	arg1	%					855:855	%	855:855	%	855:855	As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment.
34342947	1	60	theme	effective	297:305	arg1	compounds					318:326	the effective osteogenic compounds	293:326	the effective osteogenic compounds	293:326	Therefore, finding the effective osteogenic compounds for the treatment of osteoporosis is a promising research approach.
34342947	2	61	theme	sulfate	529:535	arg1	derivatives					537:547	its sulfate derivatives	525:547	its sulfate derivatives	525:547	In our study, tamarind (Tamarindus indica L.) seed polysaccharide (TSP) extracted from tamarind seed was subjected to synthesize its sulfate derivatives.
34342947	4	62	theme	potent	742:747	arg1	effects					749:755	potent effects	742:755	potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58%	742:911	As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment.
34342947	4	62	theme	potent	742:747	arg1	result					722:727	the result	718:727	the result	718:727	As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment.
34342947	3	63	theme	SEM	569:571	arg1	data					625:628	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data	550:628	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data	550:628	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data revealed that tamarind seed polysaccharide sulfate (TSPS) was successfully prepared.
34342947	6	64	theme	scientific	1265:1274	arg1	fundamentals					1276:1287	scientific fundamentals	1265:1287	scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases	1265:1401	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	4	65	theme	bone	1020:1023	arg1	mineralization					1025:1038	bone mineralization	1020:1038	bone mineralization	1020:1038	As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment.
34342947	4	66	from	concentration	933:945	arg1	weeks					924:928	four weeks	919:928	four weeks at concentration of 20 μg/mL	919:957	As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment.
34342947	0	67	theme	health	153:158	arg1	one					122:124	one	122:124	one	122:124	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	0	67	theme	health	153:158	arg1	health					153:158	the most serious public health concerns	129:167	the most serious public health concerns caused by an imbalance between bone resorption and bone formation	129:233	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	0	67	theme	health	153:158	arg1	polysaccharide					57:70	sulfated derivative of polysaccharide	34:70	sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis	34:119	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	3	68	theme	analysis	616:623	arg1	data					625:628	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data	550:628	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data	550:628	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data revealed that tamarind seed polysaccharide sulfate (TSPS) was successfully prepared.
34342947	6	69	from	novelty	1188:1194	arg1	compounds					1210:1218	osteogenic compounds	1199:1218	osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases	1199:1401	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	3	70	theme	FT-IR	562:566	arg1	data					625:628	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data	550:628	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data	550:628	The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data revealed that tamarind seed polysaccharide sulfate (TSPS) was successfully prepared.
34342947	6	71	from	T. indica	1243:1251	arg1	development					1297:1307	drug development	1292:1307	drug development	1292:1307	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	6	71	from	T. indica	1243:1251	arg1	engineering					1325:1335	bone tissue engineering	1313:1335	bone tissue engineering	1313:1335	Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.
34342947	0	72	theme	polysaccharide	57:70	arg1	activities					20:29	In vitro osteogenic activities	0:29	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation,	0:234	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	0	73	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	0	74	from	impact	248:253	arg1	population					262:271	the population	258:271	the population	258:271	In vitro osteogenic activities of sulfated derivative of polysaccharide extracted from Tamarindus indica L. Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population.
34342947	4	75	theme	ALP	1003:1005	arg1	activity					1007:1014	ALP activity	1003:1014	ALP activity	1003:1014	As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment.
32171995	0	0	theme	Bacillus	103:110	arg1	ZBT2					133:136	isolated thermophilic Bacillus licheniformis strain ZBT2	81:136	isolated thermophilic Bacillus licheniformis strain ZBT2	81:136	Statistical optimization and characterization of bacterial cellulose produced by isolated thermophilic Bacillus licheniformis strain ZBT2.
32171995	7	1	theme	water	690:694	arg1	capability					706:715	water retention capability	690:715	water retention capability	690:715	The water retention capability and moisture content properties of BC and composites were promising and also exhibited negligible protein adsorption.
32171995	10	2	theme	electron	1199:1206	arg1	microscopy					1208:1217	scanning electron microscopy	1190:1217	scanning electron microscopy with energy dispersive X-ray analysis	1190:1255	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	8	3	theme	drug	903:906	arg1	profiling					917:925	controlled drug delivery profiling	892:925	controlled drug delivery profiling	892:925	The composites also demonstrated to be consistent during controlled drug delivery profiling.
32171995	0	4	theme	thermophilic	90:101	arg1	ZBT2					133:136	isolated thermophilic Bacillus licheniformis strain ZBT2	81:136	isolated thermophilic Bacillus licheniformis strain ZBT2	81:136	Statistical optimization and characterization of bacterial cellulose produced by isolated thermophilic Bacillus licheniformis strain ZBT2.
32171995	1	5	theme	excellent	170:178	arg1	biopolymer					188:197	an excellent natural biopolymer	167:197	an excellent natural biopolymer with wide range of applications	167:229	Bacterial cellulose (BC) is an excellent natural biopolymer with wide range of applications.
32171995	1	5	theme	excellent	170:178	arg1	cellulose					149:157	Bacterial cellulose	139:157	Bacterial cellulose (BC)	139:162	Bacterial cellulose (BC) is an excellent natural biopolymer with wide range of applications.
32171995	0	6	theme	strain	126:131	arg1	ZBT2					133:136	isolated thermophilic Bacillus licheniformis strain ZBT2	81:136	isolated thermophilic Bacillus licheniformis strain ZBT2	81:136	Statistical optimization and characterization of bacterial cellulose produced by isolated thermophilic Bacillus licheniformis strain ZBT2.
32171995	10	7	with	calorimetry	1311:1321	arg1	analysis					1248:1255	energy dispersive X-ray analysis	1224:1255	energy dispersive X-ray analysis	1224:1255	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	10	8	theme	thermal	1082:1088	arg1	properties					1090:1099	The structural, morphological and thermal properties	1048:1099	The structural, morphological and thermal properties	1048:1099	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	3	9	theme	BC	388:389	arg1	form					380:383	pellicle form	371:383	pellicle form of BC (3.0 g/l)	371:399	The thermophile produced pellicle form of BC (3.0 g/l) under static conditions.
32171995	3	9	theme	BC	388:389	arg1	3.0 g/l					392:398	3.0 g/l	392:398	3.0 g/l	392:398	The thermophile produced pellicle form of BC (3.0 g/l) under static conditions.
32171995	4	10	theme	central	496:502	arg1	design					514:519	central composite design	496:519	central composite design	496:519	Statistical optimization of BC was carried out by Plackett-Burman and central composite design.
32171995	4	11	theme	Statistical	426:436	arg1	optimization					438:449	Statistical optimization	426:449	Statistical optimization of BC	426:455	Statistical optimization of BC was carried out by Plackett-Burman and central composite design.
32171995	7	12	theme	composites	759:768	arg1	properties					738:747	The water retention capability and moisture content properties	686:747	The water retention capability and moisture content properties of BC and composites	686:768	The water retention capability and moisture content properties of BC and composites were promising and also exhibited negligible protein adsorption.
32171995	7	12	theme	composites	759:768	arg1	promising					775:783	promising	775:783	promising	775:783	The water retention capability and moisture content properties of BC and composites were promising and also exhibited negligible protein adsorption.
32171995	3	13	theme	pellicle	371:378	arg1	form					380:383	pellicle form	371:383	pellicle form of BC (3.0 g/l)	371:399	The thermophile produced pellicle form of BC (3.0 g/l) under static conditions.
32171995	3	13	theme	pellicle	371:378	arg1	3.0 g/l					392:398	3.0 g/l	392:398	3.0 g/l	392:398	The thermophile produced pellicle form of BC (3.0 g/l) under static conditions.
32171995	1	14	theme	natural	180:186	arg1	biopolymer					188:197	an excellent natural biopolymer	167:197	an excellent natural biopolymer with wide range of applications	167:229	Bacterial cellulose (BC) is an excellent natural biopolymer with wide range of applications.
32171995	1	14	theme	natural	180:186	arg1	cellulose					149:157	Bacterial cellulose	139:157	Bacterial cellulose (BC)	139:162	Bacterial cellulose (BC) is an excellent natural biopolymer with wide range of applications.
32171995	11	15	theme	drug-delivery	1481:1493	arg1	systems					1495:1501	drug-delivery systems	1481:1501	drug-delivery systems	1481:1501	The study reflects the exploitation of a thermophile for development of BC which can be a preferred choice as a scaffold for tissue engineering and drug-delivery systems.
32171995	10	16	dep	fourier	1149:1155	arg1	transform					1157:1165	transform	1157:1165	transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis	1157:1330	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	11	17	theme	tissue	1458:1463	arg1	engineering					1465:1475	tissue engineering	1458:1475	tissue engineering	1458:1475	The study reflects the exploitation of a thermophile for development of BC which can be a preferred choice as a scaffold for tissue engineering and drug-delivery systems.
32171995	4	18	theme	composite	504:512	arg1	design					514:519	central composite design	496:519	central composite design	496:519	Statistical optimization of BC was carried out by Plackett-Burman and central composite design.
32171995	11	19	theme	preferred	1423:1431	arg1	choice					1433:1438	a preferred choice	1421:1438	a preferred choice	1421:1438	The study reflects the exploitation of a thermophile for development of BC which can be a preferred choice as a scaffold for tissue engineering and drug-delivery systems.
32171995	11	19	theme	preferred	1423:1431	arg1	thermophile					1374:1384	a thermophile	1372:1384	a thermophile for development of BC which can be a preferred choice as a scaffold for tissue engineering and drug-delivery systems	1372:1501	The study reflects the exploitation of a thermophile for development of BC which can be a preferred choice as a scaffold for tissue engineering and drug-delivery systems.
32171995	0	20	theme	Statistical	0:10	arg1	optimization					12:23	Statistical optimization	0:23	Statistical optimization	0:23	Statistical optimization and characterization of bacterial cellulose produced by isolated thermophilic Bacillus licheniformis strain ZBT2.
32171995	7	21	theme	capability	706:715	arg1	properties					738:747	The water retention capability and moisture content properties	686:747	The water retention capability and moisture content properties of BC and composites	686:768	The water retention capability and moisture content properties of BC and composites were promising and also exhibited negligible protein adsorption.
32171995	7	21	theme	capability	706:715	arg1	promising					775:783	promising	775:783	promising	775:783	The water retention capability and moisture content properties of BC and composites were promising and also exhibited negligible protein adsorption.
32171995	10	22	dep	spectroscopy	1176:1187	arg1	analysis					1323:1330	analysis	1323:1330	analysis	1323:1330	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	2	23	theme	strain	333:338	arg1	ZBT2					340:343	Bacillus licheniformis strain ZBT2	310:343	Bacillus licheniformis strain ZBT2	310:343	The present study reports a potential BC producing thermophile, identified as Bacillus licheniformis strain ZBT2.
32171995	1	24	theme	wide	204:207	arg1	range					209:213	wide range	204:213	wide range of applications	204:229	Bacterial cellulose (BC) is an excellent natural biopolymer with wide range of applications.
32171995	6	25	theme	hydrogels	620:628	arg1	composites					630:639	BC-gelatin hydrogels composites	609:639	BC-gelatin hydrogels composites	609:639	BC-gelatin hydrogels composites were developed to assess various properties.
32171995	2	26	theme	licheniformis	319:331	arg1	ZBT2					340:343	Bacillus licheniformis strain ZBT2	310:343	Bacillus licheniformis strain ZBT2	310:343	The present study reports a potential BC producing thermophile, identified as Bacillus licheniformis strain ZBT2.
32171995	10	27	with	microscopy	1208:1217	arg1	analysis					1248:1255	energy dispersive X-ray analysis	1224:1255	energy dispersive X-ray analysis	1224:1255	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	6	28	theme	BC-gelatin	609:618	arg1	composites					630:639	BC-gelatin hydrogels composites	609:639	BC-gelatin hydrogels composites	609:639	BC-gelatin hydrogels composites were developed to assess various properties.
32171995	10	29	theme	X-ray	1242:1246	arg1	analysis					1248:1255	energy dispersive X-ray analysis	1224:1255	energy dispersive X-ray analysis	1224:1255	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	11	30	theme	thermophile	1374:1384	arg1	exploitation					1356:1367	the exploitation	1352:1367	the exploitation of a thermophile for development of BC which can be a preferred choice as a scaffold for tissue engineering and drug-delivery systems	1352:1501	The study reflects the exploitation of a thermophile for development of BC which can be a preferred choice as a scaffold for tissue engineering and drug-delivery systems.
32171995	10	31	theme	such	1140:1143	arg1	techniques					1129:1138	analytical techniques	1118:1138	analytical techniques such as	1118:1146	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	10	31	theme	such	1140:1143	arg1	fourier					1149:1155	fourier	1149:1155	fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis	1149:1330	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	2	32	theme	Bacillus	310:317	arg1	ZBT2					340:343	Bacillus licheniformis strain ZBT2	310:343	Bacillus licheniformis strain ZBT2	310:343	The present study reports a potential BC producing thermophile, identified as Bacillus licheniformis strain ZBT2.
32171995	0	33	theme	bacterial	49:57	arg1	cellulose					59:67	bacterial cellulose	49:67	bacterial cellulose	49:67	Statistical optimization and characterization of bacterial cellulose produced by isolated thermophilic Bacillus licheniformis strain ZBT2.
32171995	10	34	theme	dispersive	1231:1240	arg1	analysis					1248:1255	energy dispersive X-ray analysis	1224:1255	energy dispersive X-ray analysis	1224:1255	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	7	35	theme	BC	752:753	arg1	properties					738:747	The water retention capability and moisture content properties	686:747	The water retention capability and moisture content properties of BC and composites	686:768	The water retention capability and moisture content properties of BC and composites were promising and also exhibited negligible protein adsorption.
32171995	7	35	theme	BC	752:753	arg1	promising					775:783	promising	775:783	promising	775:783	The water retention capability and moisture content properties of BC and composites were promising and also exhibited negligible protein adsorption.
32171995	1	36	theme	applications	218:229	arg1	range					209:213	wide range	204:213	wide range of applications	204:229	Bacterial cellulose (BC) is an excellent natural biopolymer with wide range of applications.
32171995	10	37	with	spectroscopy	1176:1187	arg1	analysis					1248:1255	energy dispersive X-ray analysis	1224:1255	energy dispersive X-ray analysis	1224:1255	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	10	38	theme	energy	1224:1229	arg1	analysis					1248:1255	energy dispersive X-ray analysis	1224:1255	energy dispersive X-ray analysis	1224:1255	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	7	39	theme	content	730:736	arg1	properties					738:747	The water retention capability and moisture content properties	686:747	The water retention capability and moisture content properties of BC and composites	686:768	The water retention capability and moisture content properties of BC and composites were promising and also exhibited negligible protein adsorption.
32171995	7	39	theme	content	730:736	arg1	promising					775:783	promising	775:783	promising	775:783	The water retention capability and moisture content properties of BC and composites were promising and also exhibited negligible protein adsorption.
32171995	10	40	theme	morphological	1064:1076	arg1	properties					1090:1099	The structural, morphological and thermal properties	1048:1099	The structural, morphological and thermal properties	1048:1099	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	3	41	theme	static	407:412	arg1	conditions					414:423	static conditions	407:423	static conditions	407:423	The thermophile produced pellicle form of BC (3.0 g/l) under static conditions.
32171995	10	42	theme	differential	1289:1300	arg1	calorimetry					1311:1321	differential scanning calorimetry	1289:1321	differential scanning calorimetry	1289:1321	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	2	43	theme	present	236:242	arg1	study					244:248	The present study	232:248	The present study	232:248	The present study reports a potential BC producing thermophile, identified as Bacillus licheniformis strain ZBT2.
32171995	1	44	theme	Bacterial	139:147	arg1	BC					160:161	BC	160:161	BC	160:161	Bacterial cellulose (BC) is an excellent natural biopolymer with wide range of applications.
32171995	1	44	theme	Bacterial	139:147	arg1	cellulose					149:157	Bacterial cellulose	139:157	Bacterial cellulose (BC)	139:162	Bacterial cellulose (BC) is an excellent natural biopolymer with wide range of applications.
32171995	1	44	theme	Bacterial	139:147	arg1	biopolymer					188:197	an excellent natural biopolymer	167:197	an excellent natural biopolymer with wide range of applications	167:229	Bacterial cellulose (BC) is an excellent natural biopolymer with wide range of applications.
32171995	0	45	theme	cellulose	59:67	arg1	characterization					29:44	characterization	29:44	characterization	29:44	Statistical optimization and characterization of bacterial cellulose produced by isolated thermophilic Bacillus licheniformis strain ZBT2.
32171995	0	45	theme	cellulose	59:67	arg1	optimization					12:23	Statistical optimization	0:23	Statistical optimization	0:23	Statistical optimization and characterization of bacterial cellulose produced by isolated thermophilic Bacillus licheniformis strain ZBT2.
32171995	7	46	theme	protein	815:821	arg1	adsorption					823:832	negligible protein adsorption	804:832	negligible protein adsorption	804:832	The water retention capability and moisture content properties of BC and composites were promising and also exhibited negligible protein adsorption.
32171995	11	47	theme	BC	1405:1406	arg1	development					1390:1400	development	1390:1400	development of BC	1390:1406	The study reflects the exploitation of a thermophile for development of BC which can be a preferred choice as a scaffold for tissue engineering and drug-delivery systems.
32171995	10	48	theme	structural	1052:1061	arg1	properties					1090:1099	The structural, morphological and thermal properties	1048:1099	The structural, morphological and thermal properties	1048:1099	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	10	49	theme	analytical	1118:1127	arg1	techniques					1129:1138	analytical techniques	1118:1138	analytical techniques such as	1118:1146	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	10	49	theme	analytical	1118:1127	arg1	fourier					1149:1155	fourier	1149:1155	fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis	1149:1330	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	10	50	theme	thermogravimetric	1258:1274	arg1	analysis					1276:1283	thermogravimetric analysis	1258:1283	thermogravimetric analysis	1258:1283	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	9	51	theme	antibacterial	974:986	arg1	efficiency					988:997	antibacterial efficiency	974:997	antibacterial efficiency	974:997	Furthermore, the composites also demonstrated antibacterial efficiency against Escherichia coli and Micrococcus luteus.
32171995	7	52	theme	moisture	721:728	arg1	content					730:736	moisture content	721:736	moisture content	721:736	The water retention capability and moisture content properties of BC and composites were promising and also exhibited negligible protein adsorption.
32171995	2	53	theme	producing	273:281	arg1	thermophile					283:293	a potential BC producing thermophile	258:293	a potential BC producing thermophile	258:293	The present study reports a potential BC producing thermophile, identified as Bacillus licheniformis strain ZBT2.
32171995	1	54	with	biopolymer	188:197	arg1	range					209:213	wide range	204:213	wide range of applications	204:229	Bacterial cellulose (BC) is an excellent natural biopolymer with wide range of applications.
32171995	7	55	theme	retention	696:704	arg1	capability					706:715	water retention capability	690:715	water retention capability	690:715	The water retention capability and moisture content properties of BC and composites were promising and also exhibited negligible protein adsorption.
32171995	2	56	theme	BC	270:271	arg1	thermophile					283:293	a potential BC producing thermophile	258:293	a potential BC producing thermophile	258:293	The present study reports a potential BC producing thermophile, identified as Bacillus licheniformis strain ZBT2.
32171995	5	57	theme	BC	543:544	arg1	9.2 g/l					553:559	9.2 g/l	553:559	9.2 g/l	553:559	Results suggest that BC yield (9.2 g/l) was enhanced with 6.6-fold after optimization.
32171995	5	57	theme	BC	543:544	arg1	yield					546:550	BC yield	543:550	BC yield (9.2 g/l)	543:560	Results suggest that BC yield (9.2 g/l) was enhanced with 6.6-fold after optimization.
32171995	8	58	theme	controlled	892:901	arg1	profiling					917:925	controlled drug delivery profiling	892:925	controlled drug delivery profiling	892:925	The composites also demonstrated to be consistent during controlled drug delivery profiling.
32171995	7	59	theme	negligible	804:813	arg1	adsorption					823:832	negligible protein adsorption	804:832	negligible protein adsorption	804:832	The water retention capability and moisture content properties of BC and composites were promising and also exhibited negligible protein adsorption.
32171995	0	60	theme	isolated	81:88	arg1	ZBT2					133:136	isolated thermophilic Bacillus licheniformis strain ZBT2	81:136	isolated thermophilic Bacillus licheniformis strain ZBT2	81:136	Statistical optimization and characterization of bacterial cellulose produced by isolated thermophilic Bacillus licheniformis strain ZBT2.
32171995	4	61	theme	BC	454:455	arg1	optimization					438:449	Statistical optimization	426:449	Statistical optimization of BC	426:455	Statistical optimization of BC was carried out by Plackett-Burman and central composite design.
32171995	2	62	theme	potential	260:268	arg1	thermophile					283:293	a potential BC producing thermophile	258:293	a potential BC producing thermophile	258:293	The present study reports a potential BC producing thermophile, identified as Bacillus licheniformis strain ZBT2.
32171995	8	63	theme	delivery	908:915	arg1	profiling					917:925	controlled drug delivery profiling	892:925	controlled drug delivery profiling	892:925	The composites also demonstrated to be consistent during controlled drug delivery profiling.
32171995	10	64	dep	transform	1157:1165	arg1	infrared					1167:1174	infrared	1167:1174	transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis	1157:1330	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	10	65	with	analysis	1276:1283	arg1	analysis					1248:1255	energy dispersive X-ray analysis	1224:1255	energy dispersive X-ray analysis	1224:1255	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	10	66	theme	scanning	1190:1197	arg1	microscopy					1208:1217	scanning electron microscopy	1190:1217	scanning electron microscopy with energy dispersive X-ray analysis	1190:1255	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	10	67	theme	scanning	1302:1309	arg1	calorimetry					1311:1321	differential scanning calorimetry	1289:1321	differential scanning calorimetry	1289:1321	The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis.
32171995	6	68	theme	various	666:672	arg1	properties					674:683	various properties	666:683	various properties	666:683	BC-gelatin hydrogels composites were developed to assess various properties.
32171995	0	69	theme	licheniformis	112:124	arg1	ZBT2					133:136	isolated thermophilic Bacillus licheniformis strain ZBT2	81:136	isolated thermophilic Bacillus licheniformis strain ZBT2	81:136	Statistical optimization and characterization of bacterial cellulose produced by isolated thermophilic Bacillus licheniformis strain ZBT2.
32204103	4	0	theme	interconnected	527:540	arg1	porosity					542:549	3D interconnected porosity	524:549	3D interconnected porosity	524:549	These composite materials, prepared by freeze-drying, exhibit 3D interconnected porosity.
32204103	0	1	theme	engineering	89:99	arg1	applications					101:112	tissue engineering applications	82:112	tissue engineering applications	82:112	Assessing the potential of boronic acid/chitosan/bioglass composite materials for tissue engineering applications.
32204103	7	2	theme	kidney	862:867	arg1	cells					918:922	Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells	825:922	Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells	825:922	Composite materials containing boronic acid show no toxicity for Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells, as opposed to composites containing non-functionalised chitosan.
32204103	1	3	theme	suitable	181:188	arg1	bioactivity					190:200	suitable bioactivity in order to promote cell proliferation and adhesion	181:252	suitable bioactivity in order to promote cell proliferation and adhesion	181:252	Composite materials aimed at bone tissue engineering need to have suitable bioactivity in order to promote cell proliferation and adhesion.
32204103	7	4	theme	bone	892:895	arg1	HS-5					912:915	HS-5	912:915	HS-5	912:915	Composite materials containing boronic acid show no toxicity for Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells, as opposed to composites containing non-functionalised chitosan.
32204103	7	4	theme	bone	892:895	arg1	marrow/stroma					897:909	Human bone marrow/stroma	886:909	Human bone marrow/stroma (HS-5)	886:916	Composite materials containing boronic acid show no toxicity for Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells, as opposed to composites containing non-functionalised chitosan.
32204103	7	5	theme	boronic	791:797	arg1	acid					799:802	boronic acid	791:802	boronic acid	791:802	Composite materials containing boronic acid show no toxicity for Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells, as opposed to composites containing non-functionalised chitosan.
32204103	7	6	theme	non-functionalised	961:978	arg1	chitosan					980:987	non-functionalised chitosan	961:987	non-functionalised chitosan	961:987	Composite materials containing boronic acid show no toxicity for Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells, as opposed to composites containing non-functionalised chitosan.
32204103	2	7	theme	boronic	298:304	arg1	acids					306:310	boronic acids	298:310	boronic acids	298:310	In this article, we study the potential of boronic acids to improve the bioactivity of chitosan-based composite materials.
32204103	2	8	theme	acids	306:310	arg1	potential					285:293	the potential	281:293	the potential of boronic acids to improve the bioactivity of chitosan-based composite materials	281:375	In this article, we study the potential of boronic acids to improve the bioactivity of chitosan-based composite materials.
32204103	7	9	theme	Composite	760:768	arg1	materials					770:778	Composite materials	760:778	Composite materials containing boronic acid	760:802	Composite materials containing boronic acid show no toxicity for Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells, as opposed to composites containing non-functionalised chitosan.
32204103	5	10	theme	x-ray	643:647	arg1	diffraction					649:659	x-ray diffraction	643:659	x-ray diffraction	643:659	The materials were fully characterized using scanning electron microscopy (SEM), FT-IR and x-ray diffraction.
32204103	7	11	theme	embryonic	852:860	arg1	kidney					862:867	Human embryonic kidney 293	846:871	Human embryonic kidney 293 (Hek293)	846:880	Composite materials containing boronic acid show no toxicity for Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells, as opposed to composites containing non-functionalised chitosan.
32204103	7	11	theme	embryonic	852:860	arg1	Hek293					874:879	Hek293	874:879	Hek293	874:879	Composite materials containing boronic acid show no toxicity for Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells, as opposed to composites containing non-functionalised chitosan.
32204103	1	12	contain	have	176:179	arg2	bioactivity					190:200	suitable bioactivity in order to promote cell proliferation and adhesion	181:252	suitable bioactivity in order to promote cell proliferation and adhesion	181:252	Composite materials aimed at bone tissue engineering need to have suitable bioactivity in order to promote cell proliferation and adhesion.
32204103	1	12	contain	have	176:179	arg1	materials					125:133	Composite materials	115:133	Composite materials aimed at bone tissue engineering	115:166	Composite materials aimed at bone tissue engineering need to have suitable bioactivity in order to promote cell proliferation and adhesion.
32204103	7	13	theme	Human	886:890	arg1	HS-5					912:915	HS-5	912:915	HS-5	912:915	Composite materials containing boronic acid show no toxicity for Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells, as opposed to composites containing non-functionalised chitosan.
32204103	7	13	theme	Human	886:890	arg1	marrow/stroma					897:909	Human bone marrow/stroma	886:909	Human bone marrow/stroma (HS-5)	886:916	Composite materials containing boronic acid show no toxicity for Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells, as opposed to composites containing non-functionalised chitosan.
32204103	0	14	theme	composite	58:66	arg1	materials					68:76	boronic acid/chitosan/bioglass composite materials	27:76	boronic acid/chitosan/bioglass composite materials	27:76	Assessing the potential of boronic acid/chitosan/bioglass composite materials for tissue engineering applications.
32204103	4	15	theme	3D	524:525	arg1	porosity					542:549	3D interconnected porosity	524:549	3D interconnected porosity	524:549	These composite materials, prepared by freeze-drying, exhibit 3D interconnected porosity.
32204103	6	16	theme	cytotoxicity	739:750	arg1	assays					752:757	cell cytotoxicity assays	734:757	cell cytotoxicity assays	734:757	Their bioactivity was assessed by immersion in simulated body fluid and cell cytotoxicity assays.
32204103	3	17	theme	boronic	406:412	arg1	45S5					456:459	boronic acid functionalised chitosan and Bioglass 45S5	406:459	boronic acid functionalised chitosan and Bioglass 45S5	406:459	Samples were prepared using boronic acid functionalised chitosan and Bioglass 45S5.
32204103	6	18	theme	cell	734:737	arg1	assays					752:757	cell cytotoxicity assays	734:757	cell cytotoxicity assays	734:757	Their bioactivity was assessed by immersion in simulated body fluid and cell cytotoxicity assays.
32204103	3	19	theme	acid	414:417	arg1	45S5					456:459	boronic acid functionalised chitosan and Bioglass 45S5	406:459	boronic acid functionalised chitosan and Bioglass 45S5	406:459	Samples were prepared using boronic acid functionalised chitosan and Bioglass 45S5.
32204103	1	20	theme	Composite	115:123	arg1	materials					125:133	Composite materials	115:133	Composite materials aimed at bone tissue engineering	115:166	Composite materials aimed at bone tissue engineering need to have suitable bioactivity in order to promote cell proliferation and adhesion.
32204103	7	21	contain	containing	950:959	arg1	composites					939:948	composites	939:948	composites containing non-functionalised chitosan	939:987	Composite materials containing boronic acid show no toxicity for Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells, as opposed to composites containing non-functionalised chitosan.
32204103	7	21	contain	containing	950:959	arg2	chitosan					980:987	non-functionalised chitosan	961:987	non-functionalised chitosan	961:987	Composite materials containing boronic acid show no toxicity for Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells, as opposed to composites containing non-functionalised chitosan.
32204103	7	22	theme	Human	846:850	arg1	kidney					862:867	Human embryonic kidney 293	846:871	Human embryonic kidney 293 (Hek293)	846:880	Composite materials containing boronic acid show no toxicity for Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells, as opposed to composites containing non-functionalised chitosan.
32204103	7	22	theme	Human	846:850	arg1	Hek293					874:879	Hek293	874:879	Hek293	874:879	Composite materials containing boronic acid show no toxicity for Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells, as opposed to composites containing non-functionalised chitosan.
32204103	2	23	theme	materials	367:375	arg1	bioactivity					327:337	the bioactivity	323:337	the bioactivity of chitosan-based composite materials	323:375	In this article, we study the potential of boronic acids to improve the bioactivity of chitosan-based composite materials.
32204103	6	24	theme	body	719:722	arg1	fluid					724:728	simulated body fluid	709:728	simulated body fluid	709:728	Their bioactivity was assessed by immersion in simulated body fluid and cell cytotoxicity assays.
32204103	0	25	theme	acid/chitosan/bioglass	35:56	arg1	materials					68:76	boronic acid/chitosan/bioglass composite materials	27:76	boronic acid/chitosan/bioglass composite materials	27:76	Assessing the potential of boronic acid/chitosan/bioglass composite materials for tissue engineering applications.
32204103	2	26	theme	chitosan-based	342:355	arg1	materials					367:375	chitosan-based composite materials	342:375	chitosan-based composite materials	342:375	In this article, we study the potential of boronic acids to improve the bioactivity of chitosan-based composite materials.
32204103	6	27	theme	simulated	709:717	arg1	fluid					724:728	simulated body fluid	709:728	simulated body fluid	709:728	Their bioactivity was assessed by immersion in simulated body fluid and cell cytotoxicity assays.
32204103	0	28	theme	boronic	27:33	arg1	materials					68:76	boronic acid/chitosan/bioglass composite materials	27:76	boronic acid/chitosan/bioglass composite materials	27:76	Assessing the potential of boronic acid/chitosan/bioglass composite materials for tissue engineering applications.
32204103	7	29	theme	Sertoli	831:837	arg1	cells					918:922	Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells	825:922	Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells	825:922	Composite materials containing boronic acid show no toxicity for Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells, as opposed to composites containing non-functionalised chitosan.
32204103	3	30	theme	Bioglass	447:454	arg1	45S5					456:459	boronic acid functionalised chitosan and Bioglass 45S5	406:459	boronic acid functionalised chitosan and Bioglass 45S5	406:459	Samples were prepared using boronic acid functionalised chitosan and Bioglass 45S5.
32204103	0	31	theme	materials	68:76	arg1	potential					14:22	the potential	10:22	the potential of boronic acid/chitosan/bioglass composite materials for tissue engineering applications	10:112	Assessing the potential of boronic acid/chitosan/bioglass composite materials for tissue engineering applications.
32204103	5	32	theme	scanning	597:604	arg1	SEM					627:629	SEM	627:629	SEM	627:629	The materials were fully characterized using scanning electron microscopy (SEM), FT-IR and x-ray diffraction.
32204103	5	32	theme	scanning	597:604	arg1	microscopy					615:624	scanning electron microscopy	597:624	scanning electron microscopy (SEM)	597:630	The materials were fully characterized using scanning electron microscopy (SEM), FT-IR and x-ray diffraction.
32204103	2	33	theme	composite	357:365	arg1	materials					367:375	chitosan-based composite materials	342:375	chitosan-based composite materials	342:375	In this article, we study the potential of boronic acids to improve the bioactivity of chitosan-based composite materials.
32204103	3	34	theme	chitosan	434:441	arg1	45S5					456:459	boronic acid functionalised chitosan and Bioglass 45S5	406:459	boronic acid functionalised chitosan and Bioglass 45S5	406:459	Samples were prepared using boronic acid functionalised chitosan and Bioglass 45S5.
32204103	7	35	contain	containing	780:789	arg1	materials					770:778	Composite materials	760:778	Composite materials containing boronic acid	760:802	Composite materials containing boronic acid show no toxicity for Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells, as opposed to composites containing non-functionalised chitosan.
32204103	7	35	contain	containing	780:789	arg2	acid					799:802	boronic acid	791:802	boronic acid	791:802	Composite materials containing boronic acid show no toxicity for Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells, as opposed to composites containing non-functionalised chitosan.
32204103	5	36	theme	electron	606:613	arg1	SEM					627:629	SEM	627:629	SEM	627:629	The materials were fully characterized using scanning electron microscopy (SEM), FT-IR and x-ray diffraction.
32204103	5	36	theme	electron	606:613	arg1	microscopy					615:624	scanning electron microscopy	597:624	scanning electron microscopy (SEM)	597:630	The materials were fully characterized using scanning electron microscopy (SEM), FT-IR and x-ray diffraction.
32204103	1	37	theme	bone	144:147	arg1	engineering					156:166	bone tissue engineering	144:166	bone tissue engineering	144:166	Composite materials aimed at bone tissue engineering need to have suitable bioactivity in order to promote cell proliferation and adhesion.
32204103	7	38	theme	Mouse	825:829	arg1	Sertoli					831:837	Mouse Sertoli	825:837	Mouse Sertoli (TM4)	825:843	Composite materials containing boronic acid show no toxicity for Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells, as opposed to composites containing non-functionalised chitosan.
32204103	7	38	theme	Mouse	825:829	arg1	TM4					840:842	TM4	840:842	TM4	840:842	Composite materials containing boronic acid show no toxicity for Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells, as opposed to composites containing non-functionalised chitosan.
32204103	1	39	theme	tissue	149:154	arg1	engineering					156:166	bone tissue engineering	144:166	bone tissue engineering	144:166	Composite materials aimed at bone tissue engineering need to have suitable bioactivity in order to promote cell proliferation and adhesion.
32204103	4	40	theme	composite	468:476	arg1	materials					478:486	These composite materials	462:486	These composite materials	462:486	These composite materials, prepared by freeze-drying, exhibit 3D interconnected porosity.
32204103	0	41	theme	tissue	82:87	arg1	applications					101:112	tissue engineering applications	82:112	tissue engineering applications	82:112	Assessing the potential of boronic acid/chitosan/bioglass composite materials for tissue engineering applications.
32204103	7	42	theme	marrow/stroma	897:909	arg1	cells					918:922	Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells	825:922	Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells	825:922	Composite materials containing boronic acid show no toxicity for Mouse Sertoli (TM4), Human embryonic kidney 293 (Hek293) and Human bone marrow/stroma (HS-5) cells, as opposed to composites containing non-functionalised chitosan.
32204103	3	43	theme	functionalised	419:432	arg1	45S5					456:459	boronic acid functionalised chitosan and Bioglass 45S5	406:459	boronic acid functionalised chitosan and Bioglass 45S5	406:459	Samples were prepared using boronic acid functionalised chitosan and Bioglass 45S5.
32204103	1	44	dep	cell	222:225	arg1	adhesion					245:252	adhesion	245:252	adhesion	245:252	Composite materials aimed at bone tissue engineering need to have suitable bioactivity in order to promote cell proliferation and adhesion.
32204103	1	44	dep	cell	222:225	arg1	proliferation					227:239	proliferation	227:239	proliferation	227:239	Composite materials aimed at bone tissue engineering need to have suitable bioactivity in order to promote cell proliferation and adhesion.
32649905	3	0	theme	lipoprotein	726:736	arg1	endocytosis					699:709	the endocytosis	695:709	the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6)	695:770	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	1	gly	-fucosylation	547:559	arg1	GlcNAc					585:590	GlcNAc	585:590	GlcNAc	585:590	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	1	gly	-fucosylation	547:559	arg1	N-acetylglucosamine					564:582	N-acetylglucosamine	564:582	N-acetylglucosamine (GlcNAc)	564:591	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	1	gly	-fucosylation	547:559	arg1	sequences					617:625	the Galβ(1-4)-GlcNAc sequences	596:625	the Galβ(1-4)-GlcNAc sequences of complex N-glycans	596:646	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	2	from	-fucosylation	547:559	arg1	sequences					617:625	the Galβ(1-4)-GlcNAc sequences	596:625	the Galβ(1-4)-GlcNAc sequences of complex N-glycans	596:646	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	6	3	theme	in	1040:1041	arg1	fucosylation					1061:1072	in situ cell-surface fucosylation	1040:1072	in situ cell-surface fucosylation	1040:1072	The combined data show that in situ cell-surface fucosylation can be exploited to regulate a specific signaling pathway via endocytosis promoted by a fucose-binding protein, thereby linking glycosylation of a receptor with its intracellular signaling.
32649905	6	4	dep	in	1040:1041	arg1	situ					1043:1046	situ	1043:1046	situ	1043:1046	The combined data show that in situ cell-surface fucosylation can be exploited to regulate a specific signaling pathway via endocytosis promoted by a fucose-binding protein, thereby linking glycosylation of a receptor with its intracellular signaling.
32649905	4	5	theme	signaling	908:916	arg1	suppression					879:889	the suppression	875:889	the suppression of Wnt/β-catenin signaling	875:916	Pulse-chase experiments reveal that ISF elevates endocytosis of lipid-raft-localized LRP6, leading to the suppression of Wnt/β-catenin signaling.
32649905	1	6	from	transcription	174:186	arg1	events					214:219	numerous physiological events	191:219	numerous physiological events	191:219	Wnt/β-catenin signaling regulates critical, context-dependent transcription in numerous physiological events.
32649905	3	7	theme	α	541:541	arg1	-fucosylation					547:559	α(1-3)-fucosylation	541:559	α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans	541:646	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	0	8	theme	Wnt/β-Catenin	87:99	arg1	Signaling					101:109	Wnt/β-Catenin Signaling	87:109	Wnt/β-Catenin Signaling	87:109	In Situ Fucosylation of the Wnt Co-receptor LRP6 Increases Its Endocytosis and Reduces Wnt/β-Catenin Signaling.
32649905	0	9	gly	Fucosylation	8:19	arg1	LRP6					44:47	the Wnt Co-receptor LRP6	24:47	the Wnt Co-receptor LRP6	24:47	In Situ Fucosylation of the Wnt Co-receptor LRP6 Increases Its Endocytosis and Reduces Wnt/β-Catenin Signaling.
32649905	3	10	theme	ovary	410:414	arg1	mutants					421:427	Chinese hamster ovary cell mutants	394:427	Chinese hamster ovary cell mutants with different fucosylation levels	394:462	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	11	theme	N-acetylglucosamine	564:582	arg1	-fucosylation					547:559	α(1-3)-fucosylation	541:559	α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans	541:646	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	1	12	dep	critical	146:153	arg1	context-dependent					156:172	context-dependent	156:172	context-dependent	156:172	Wnt/β-catenin signaling regulates critical, context-dependent transcription in numerous physiological events.
32649905	6	13	theme	receptor	1221:1228	arg1	glycosylation					1202:1214	glycosylation	1202:1214	glycosylation of a receptor	1202:1228	The combined data show that in situ cell-surface fucosylation can be exploited to regulate a specific signaling pathway via endocytosis promoted by a fucose-binding protein, thereby linking glycosylation of a receptor with its intracellular signaling.
32649905	6	14	theme	specific	1105:1112	arg1	pathway					1124:1130	a specific signaling pathway	1103:1130	a specific signaling pathway	1103:1130	The combined data show that in situ cell-surface fucosylation can be exploited to regulate a specific signaling pathway via endocytosis promoted by a fucose-binding protein, thereby linking glycosylation of a receptor with its intracellular signaling.
32649905	1	15	theme	numerous	191:198	arg1	events					214:219	numerous physiological events	191:219	numerous physiological events	191:219	Wnt/β-catenin signaling regulates critical, context-dependent transcription in numerous physiological events.
32649905	3	16	theme	Chinese	394:400	arg1	mutants					421:427	Chinese hamster ovary cell mutants	394:427	Chinese hamster ovary cell mutants with different fucosylation levels	394:462	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	17	theme	receptor-related	738:753	arg1	protein					755:761	receptor-related protein 6	738:763	low-density lipoprotein receptor-related protein 6 (LRP6)	714:770	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	17	theme	receptor-related	738:753	arg1	LRP6					766:769	LRP6	766:769	LRP6	766:769	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	18	theme	different	434:442	arg1	levels					457:462	different fucosylation levels	434:462	different fucosylation levels	434:462	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	1	19	theme	physiological	200:212	arg1	events					214:219	numerous physiological events	191:219	numerous physiological events	191:219	Wnt/β-catenin signaling regulates critical, context-dependent transcription in numerous physiological events.
32649905	6	20	gly	glycosylation	1202:1214	arg1	receptor					1221:1228	a receptor	1219:1228	a receptor	1219:1228	The combined data show that in situ cell-surface fucosylation can be exploited to regulate a specific signaling pathway via endocytosis promoted by a fucose-binding protein, thereby linking glycosylation of a receptor with its intracellular signaling.
32649905	4	21	theme	Wnt/β-catenin	894:906	arg1	signaling					908:916	Wnt/β-catenin signaling	894:916	Wnt/β-catenin signaling	894:916	Pulse-chase experiments reveal that ISF elevates endocytosis of lipid-raft-localized LRP6, leading to the suppression of Wnt/β-catenin signaling.
32649905	3	22	theme	fucose	481:486	arg1	[ISF					518:521	in situ fucosylation [ISF	497:521	in situ fucosylation [ISF	497:521	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	22	theme	fucose	481:486	arg1	editing					488:494	cell-surface fucose editing	468:494	cell-surface fucose editing (in situ fucosylation [ISF])	468:523	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	0	23	theme	In	0:1	arg1	Fucosylation					8:19	In Situ Fucosylation	0:19	In Situ Fucosylation of the Wnt Co-receptor LRP6	0:47	In Situ Fucosylation of the Wnt Co-receptor LRP6 Increases Its Endocytosis and Reduces Wnt/β-Catenin Signaling.
32649905	6	24	theme	combined	1016:1023	arg1	data					1025:1028	The combined data	1012:1028	The combined data	1012:1028	The combined data show that in situ cell-surface fucosylation can be exploited to regulate a specific signaling pathway via endocytosis promoted by a fucose-binding protein, thereby linking glycosylation of a receptor with its intracellular signaling.
32649905	3	25	with	mutants	421:427	arg1	levels					457:462	different fucosylation levels	434:462	different fucosylation levels	434:462	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	26	theme	cell	416:419	arg1	mutants					421:427	Chinese hamster ovary cell mutants	394:427	Chinese hamster ovary cell mutants with different fucosylation levels	394:462	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	6	27	theme	signaling	1114:1122	arg1	pathway					1124:1130	a specific signaling pathway	1103:1130	a specific signaling pathway	1103:1130	The combined data show that in situ cell-surface fucosylation can be exploited to regulate a specific signaling pathway via endocytosis promoted by a fucose-binding protein, thereby linking glycosylation of a receptor with its intracellular signaling.
32649905	3	28	dep	in	497:498	arg1	situ					500:503	situ	500:503	situ	500:503	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	4	29	theme	Pulse-chase	773:783	arg1	experiments					785:795	Pulse-chase experiments	773:795	Pulse-chase experiments	773:795	Pulse-chase experiments reveal that ISF elevates endocytosis of lipid-raft-localized LRP6, leading to the suppression of Wnt/β-catenin signaling.
32649905	3	30	theme	mutants	421:427	arg1	combination					379:389	a combination	377:389	a combination of Chinese hamster ovary cell mutants with different fucosylation levels	377:462	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	30	theme	mutants	421:427	arg1	[ISF					518:521	in situ fucosylation [ISF	497:521	in situ fucosylation [ISF	497:521	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	30	theme	mutants	421:427	arg1	editing					488:494	cell-surface fucose editing	468:494	cell-surface fucose editing (in situ fucosylation [ISF])	468:523	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	5	31	theme	added	998:1002	arg1	fucose					1004:1009	the exogenously added fucose	982:1009	the exogenously added fucose	982:1009	Remarkably, Wnt activity decreased by ISF is fully reversed by the exogenously added fucose.
32649905	0	32	theme	Wnt	28:30	arg1	LRP6					44:47	the Wnt Co-receptor LRP6	24:47	the Wnt Co-receptor LRP6	24:47	In Situ Fucosylation of the Wnt Co-receptor LRP6 Increases Its Endocytosis and Reduces Wnt/β-Catenin Signaling.
32649905	3	33	dep	lipoprotein	726:736	arg1	protein					755:761	receptor-related protein 6	738:763	low-density lipoprotein receptor-related protein 6 (LRP6)	714:770	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	33	dep	lipoprotein	726:736	arg1	LRP6					766:769	LRP6	766:769	LRP6	766:769	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	34	theme	Wnt/β-catenin	658:670	arg1	activity					672:679	Wnt/β-catenin activity	658:679	Wnt/β-catenin activity	658:679	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	6	35	theme	intracellular	1239:1251	arg1	signaling					1253:1261	its intracellular signaling	1235:1261	its intracellular signaling	1235:1261	The combined data show that in situ cell-surface fucosylation can be exploited to regulate a specific signaling pathway via endocytosis promoted by a fucose-binding protein, thereby linking glycosylation of a receptor with its intracellular signaling.
32649905	3	36	theme	cell-surface	468:479	arg1	[ISF					518:521	in situ fucosylation [ISF	497:521	in situ fucosylation [ISF	497:521	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	36	theme	cell-surface	468:479	arg1	editing					488:494	cell-surface fucose editing	468:494	cell-surface fucose editing (in situ fucosylation [ISF])	468:523	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	37	theme	complex	630:636	arg1	N-glycans					638:646	complex N-glycans	630:646	complex N-glycans	630:646	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	4	38	theme	LRP6	858:861	arg1	endocytosis					822:832	endocytosis	822:832	endocytosis of lipid-raft-localized LRP6	822:861	Pulse-chase experiments reveal that ISF elevates endocytosis of lipid-raft-localized LRP6, leading to the suppression of Wnt/β-catenin signaling.
32649905	3	39	theme	fucosylation	505:516	arg1	[ISF					518:521	in situ fucosylation [ISF	497:521	in situ fucosylation [ISF	497:521	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	39	theme	fucosylation	505:516	arg1	editing					488:494	cell-surface fucose editing	468:494	cell-surface fucose editing (in situ fucosylation [ISF])	468:523	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	0	40	theme	LRP6	44:47	arg1	Fucosylation					8:19	In Situ Fucosylation	0:19	In Situ Fucosylation of the Wnt Co-receptor LRP6	0:47	In Situ Fucosylation of the Wnt Co-receptor LRP6 Increases Its Endocytosis and Reduces Wnt/β-Catenin Signaling.
32649905	3	41	theme	hamster	402:408	arg1	mutants					421:427	Chinese hamster ovary cell mutants	394:427	Chinese hamster ovary cell mutants with different fucosylation levels	394:462	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	42	theme	N-glycans	638:646	arg1	sequences					617:625	the Galβ(1-4)-GlcNAc sequences	596:625	the Galβ(1-4)-GlcNAc sequences of complex N-glycans	596:646	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	2	43	theme	Wnt/β-catenin	269:281	arg1	activity					283:290	Wnt/β-catenin activity	269:290	Wnt/β-catenin activity	269:290	Among the well-documented mechanisms affecting Wnt/β-catenin activity, modification of N-glycans by L-fucose is the newest and the least understood.
32649905	1	44	theme	Wnt/β-catenin	112:124	arg1	signaling					126:134	Wnt/β-catenin signaling	112:134	Wnt/β-catenin signaling	112:134	Wnt/β-catenin signaling regulates critical, context-dependent transcription in numerous physiological events.
32649905	0	45	theme	Co-receptor	32:42	arg1	LRP6					44:47	the Wnt Co-receptor LRP6	24:47	the Wnt Co-receptor LRP6	24:47	In Situ Fucosylation of the Wnt Co-receptor LRP6 Increases Its Endocytosis and Reduces Wnt/β-Catenin Signaling.
32649905	3	46	theme	Galβ	600:603	arg1	sequences					617:625	the Galβ(1-4)-GlcNAc sequences	596:625	the Galβ(1-4)-GlcNAc sequences of complex N-glycans	596:646	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	47	theme	-GlcNAc	609:615	arg1	sequences					617:625	the Galβ(1-4)-GlcNAc sequences	596:625	the Galβ(1-4)-GlcNAc sequences of complex N-glycans	596:646	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	2	48	theme	well-documented	232:246	arg1	mechanisms					248:257	the well-documented mechanisms	228:257	the well-documented mechanisms affecting Wnt/β-catenin activity	228:290	Among the well-documented mechanisms affecting Wnt/β-catenin activity, modification of N-glycans by L-fucose is the newest and the least understood.
32649905	6	49	theme	fucose-binding	1162:1175	arg1	protein					1177:1183	a fucose-binding protein	1160:1183	a fucose-binding protein	1160:1183	The combined data show that in situ cell-surface fucosylation can be exploited to regulate a specific signaling pathway via endocytosis promoted by a fucose-binding protein, thereby linking glycosylation of a receptor with its intracellular signaling.
32649905	2	50	theme	N-glycans	309:317	arg1	newest					338:343	newest	338:343	newest	338:343	Among the well-documented mechanisms affecting Wnt/β-catenin activity, modification of N-glycans by L-fucose is the newest and the least understood.
32649905	2	50	theme	N-glycans	309:317	arg1	modification					293:304	modification	293:304	modification of N-glycans by L-fucose	293:329	Among the well-documented mechanisms affecting Wnt/β-catenin activity, modification of N-glycans by L-fucose is the newest and the least understood.
32649905	1	51	theme	critical	146:153	arg1	transcription					174:186	critical, context-dependent transcription	146:186	critical, context-dependent transcription in numerous physiological events	146:219	Wnt/β-catenin signaling regulates critical, context-dependent transcription in numerous physiological events.
32649905	4	52	theme	lipid-raft-localized	837:856	arg1	LRP6					858:861	lipid-raft-localized LRP6	837:861	lipid-raft-localized LRP6	837:861	Pulse-chase experiments reveal that ISF elevates endocytosis of lipid-raft-localized LRP6, leading to the suppression of Wnt/β-catenin signaling.
32649905	5	53	theme	Wnt	931:933	arg1	activity					935:942	Wnt activity	931:942	Wnt activity decreased by ISF	931:959	Remarkably, Wnt activity decreased by ISF is fully reversed by the exogenously added fucose.
32649905	3	54	theme	in	497:498	arg1	[ISF					518:521	in situ fucosylation [ISF	497:521	in situ fucosylation [ISF	497:521	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	54	theme	in	497:498	arg1	editing					488:494	cell-surface fucose editing	468:494	cell-surface fucose editing (in situ fucosylation [ISF])	468:523	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	6	55	theme	cell-surface	1048:1059	arg1	fucosylation					1061:1072	in situ cell-surface fucosylation	1040:1072	in situ cell-surface fucosylation	1040:1072	The combined data show that in situ cell-surface fucosylation can be exploited to regulate a specific signaling pathway via endocytosis promoted by a fucose-binding protein, thereby linking glycosylation of a receptor with its intracellular signaling.
32649905	3	56	theme	fucosylation	444:455	arg1	levels					457:462	different fucosylation levels	434:462	different fucosylation levels	434:462	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	3	57	theme	low-density	714:724	arg1	lipoprotein					726:736	low-density lipoprotein receptor-related protein 6 (LRP6)	714:770	low-density lipoprotein receptor-related protein 6 (LRP6)	714:770	Using a combination of Chinese hamster ovary cell mutants with different fucosylation levels and cell-surface fucose editing (in situ fucosylation [ISF]), we report that α(1-3)-fucosylation of N-acetylglucosamine (GlcNAc) in the Galβ(1-4)-GlcNAc sequences of complex N-glycans modulates Wnt/β-catenin activity by regulating the endocytosis of low-density lipoprotein receptor-related protein 6 (LRP6).
32649905	0	58	dep	In	0:1	arg1	Situ					3:6	Situ	3:6	Situ	3:6	In Situ Fucosylation of the Wnt Co-receptor LRP6 Increases Its Endocytosis and Reduces Wnt/β-Catenin Signaling.
35361316	1	0	theme	proximal	305:312	arg1	composition					314:324	proximal composition	305:324	proximal composition	305:324	Taro mucilage, a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott, was extracted and characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, and proximal composition.
35361316	0	1	theme	Cosmetic	64:71	arg1	Formulations					73:84	Cosmetic Formulations	64:84	Cosmetic Formulations	64:84	Taro Mucilage: Extraction, Characterization, and Application in Cosmetic Formulations.
35361316	6	2	theme	commercial	948:957	arg1	tool					1014:1017	a strategic tool	1002:1017	a strategic tool for the creation of a new product chain and adding value to the culture of Colocasia0 esculenta	1002:1113	The mucilage demonstrates potential for application in cosmetic products, and its commercial use as an ingredient in cosmetics could be a strategic tool for the creation of a new product chain and adding value to the culture of Colocasia0 esculenta.
35361316	6	2	theme	commercial	948:957	arg1	use					959:961	its commercial use	944:961	its commercial use as an ingredient in cosmetics	944:991	The mucilage demonstrates potential for application in cosmetic products, and its commercial use as an ingredient in cosmetics could be a strategic tool for the creation of a new product chain and adding value to the culture of Colocasia0 esculenta.
35361316	1	3	theme	Colocasia	143:151	arg1	Schott					168:173	Colocasia esculenta (L.) Schott	143:173	Colocasia esculenta (L.) Schott	143:173	Taro mucilage, a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott, was extracted and characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, and proximal composition.
35361316	1	4	attach	present	117:123	arg1	rhizome					132:138	the rhizome	128:138	the rhizome of Colocasia esculenta (L.) Schott	128:173	Taro mucilage, a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott, was extracted and characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, and proximal composition.
35361316	1	4	attach	present	117:123	arg2	mucilage					92:99	Taro mucilage	87:99	Taro mucilage	87:99	Taro mucilage, a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott, was extracted and characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, and proximal composition.
35361316	1	4	attach	present	117:123	arg2	hydrocolloid					104:115	a hydrocolloid	102:115	a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott	102:173	Taro mucilage, a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott, was extracted and characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, and proximal composition.
35361316	1	5	theme	X-ray	234:238	arg1	diffraction					240:250	X-ray diffraction	234:250	X-ray diffraction	234:250	Taro mucilage, a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott, was extracted and characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, and proximal composition.
35361316	3	6	theme	surface	533:539	arg1	area					541:544	its surface area	529:544	its surface area	529:544	The mucilage presented a semicrystalline structure with high thermal stability, the presence of granules along its surface area, and good emulsifying activity.
35361316	6	7	from	ingredient	969:978	arg1	cosmetics					983:991	cosmetics	983:991	cosmetics	983:991	The mucilage demonstrates potential for application in cosmetic products, and its commercial use as an ingredient in cosmetics could be a strategic tool for the creation of a new product chain and adding value to the culture of Colocasia0 esculenta.
35361316	1	8	from	rhizome	132:138	arg1	present					117:123	present	117:123	present	117:123	Taro mucilage, a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott, was extracted and characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, and proximal composition.
35361316	3	9	theme	granules	514:521	arg1	stability					487:495	high thermal stability	474:495	high thermal stability	474:495	The mucilage presented a semicrystalline structure with high thermal stability, the presence of granules along its surface area, and good emulsifying activity.
35361316	3	9	theme	granules	514:521	arg1	presence					502:509	the presence	498:509	the presence of granules along its surface area	498:544	The mucilage presented a semicrystalline structure with high thermal stability, the presence of granules along its surface area, and good emulsifying activity.
35361316	3	9	theme	granules	514:521	arg1	activity					568:575	good emulsifying activity	551:575	good emulsifying activity	551:575	The mucilage presented a semicrystalline structure with high thermal stability, the presence of granules along its surface area, and good emulsifying activity.
35361316	1	10	theme	esculenta	153:161	arg1	Schott					168:173	Colocasia esculenta (L.) Schott	143:173	Colocasia esculenta (L.) Schott	143:173	Taro mucilage, a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott, was extracted and characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, and proximal composition.
35361316	0	11	from	Characterization	27:42	arg1	Formulations					73:84	Cosmetic Formulations	64:84	Cosmetic Formulations	64:84	Taro Mucilage: Extraction, Characterization, and Application in Cosmetic Formulations.
35361316	1	12	from	present	117:123	arg1	rhizome					132:138	the rhizome	128:138	the rhizome of Colocasia esculenta (L.) Schott	128:173	Taro mucilage, a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott, was extracted and characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, and proximal composition.
35361316	6	13	theme	chain	1053:1057	arg1	creation					1027:1034	the creation	1023:1034	the creation of a new product chain and adding value to the culture of Colocasia0 esculenta	1023:1113	The mucilage demonstrates potential for application in cosmetic products, and its commercial use as an ingredient in cosmetics could be a strategic tool for the creation of a new product chain and adding value to the culture of Colocasia0 esculenta.
35361316	6	14	theme	new	1041:1043	arg1	chain					1053:1057	a new product chain and adding value	1039:1074	chain	1053:1057	The mucilage demonstrates potential for application in cosmetic products, and its commercial use as an ingredient in cosmetics could be a strategic tool for the creation of a new product chain and adding value to the culture of Colocasia0 esculenta.
35361316	5	15	theme	lower	767:771	arg1	index					761:765	a flow behavior index	745:765	a flow behavior index lower than 1, which is a desirable characteristic for cosmetics	745:829	All cream samples presented pseudoplastic behavior, with a flow behavior index lower than 1, which is a desirable characteristic for cosmetics, as it improves its applicability.
35361316	6	16	theme	product	1045:1051	arg1	chain					1053:1057	a new product chain and adding value	1039:1074	chain	1053:1057	The mucilage demonstrates potential for application in cosmetic products, and its commercial use as an ingredient in cosmetics could be a strategic tool for the creation of a new product chain and adding value to the culture of Colocasia0 esculenta.
35361316	1	17	theme	L.	164:165	arg1	Schott					168:173	Colocasia esculenta (L.) Schott	143:173	Colocasia esculenta (L.) Schott	143:173	Taro mucilage, a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott, was extracted and characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, and proximal composition.
35361316	0	18	theme	Taro	0:3	arg1	Mucilage					5:12	Taro Mucilage	0:12	Taro Mucilage: Extraction, Characterization, and Application in Cosmetic Formulations.	0:85	Taro Mucilage: Extraction, Characterization, and Application in Cosmetic Formulations.
35361316	1	19	theme	Taro	87:90	arg1	mucilage					92:99	Taro mucilage	87:99	Taro mucilage	87:99	Taro mucilage, a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott, was extracted and characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, and proximal composition.
35361316	1	19	theme	Taro	87:90	arg1	hydrocolloid					104:115	a hydrocolloid	102:115	a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott	102:173	Taro mucilage, a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott, was extracted and characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, and proximal composition.
35361316	4	20	theme	cosmetic	650:657	arg1	formulations					659:670	the cosmetic formulations	646:670	the cosmetic formulations	646:670	High physical-chemical stability was also found in the mucilage and the cosmetic formulations during storage.
35361316	5	21	theme	desirable	792:800	arg1	1					778:778	1	778:778	1	778:778	All cream samples presented pseudoplastic behavior, with a flow behavior index lower than 1, which is a desirable characteristic for cosmetics, as it improves its applicability.
35361316	5	21	theme	desirable	792:800	arg1	characteristic					802:815	a desirable characteristic	790:815	a desirable characteristic for cosmetics	790:829	All cream samples presented pseudoplastic behavior, with a flow behavior index lower than 1, which is a desirable characteristic for cosmetics, as it improves its applicability.
35361316	1	22	theme	scanning	253:260	arg1	microscopy					271:280	scanning electron microscopy	253:280	scanning electron microscopy	253:280	Taro mucilage, a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott, was extracted and characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, and proximal composition.
35361316	5	23	theme	cream	692:696	arg1	samples					698:704	All cream samples	688:704	All cream samples	688:704	All cream samples presented pseudoplastic behavior, with a flow behavior index lower than 1, which is a desirable characteristic for cosmetics, as it improves its applicability.
35361316	6	24	theme	esculenta	1105:1113	arg1	culture					1083:1089	the culture	1079:1089	the culture of Colocasia0 esculenta	1079:1113	The mucilage demonstrates potential for application in cosmetic products, and its commercial use as an ingredient in cosmetics could be a strategic tool for the creation of a new product chain and adding value to the culture of Colocasia0 esculenta.
35361316	1	25	theme	Schott	168:173	arg1	rhizome					132:138	the rhizome	128:138	the rhizome of Colocasia esculenta (L.) Schott	128:173	Taro mucilage, a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott, was extracted and characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, and proximal composition.
35361316	3	26	theme	good	551:554	arg1	activity					568:575	good emulsifying activity	551:575	good emulsifying activity	551:575	The mucilage presented a semicrystalline structure with high thermal stability, the presence of granules along its surface area, and good emulsifying activity.
35361316	1	27	theme	electron	262:269	arg1	microscopy					271:280	scanning electron microscopy	253:280	scanning electron microscopy	253:280	Taro mucilage, a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott, was extracted and characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, and proximal composition.
35361316	6	28	theme	Colocasia0	1094:1103	arg1	esculenta					1105:1113	Colocasia0 esculenta	1094:1113	Colocasia0 esculenta	1094:1113	The mucilage demonstrates potential for application in cosmetic products, and its commercial use as an ingredient in cosmetics could be a strategic tool for the creation of a new product chain and adding value to the culture of Colocasia0 esculenta.
35361316	6	29	theme	value	1070:1074	arg1	creation					1027:1034	the creation	1023:1034	the creation of a new product chain and adding value to the culture of Colocasia0 esculenta	1023:1113	The mucilage demonstrates potential for application in cosmetic products, and its commercial use as an ingredient in cosmetics could be a strategic tool for the creation of a new product chain and adding value to the culture of Colocasia0 esculenta.
35361316	6	30	from	potential	892:900	arg1	products					930:937	cosmetic products	921:937	cosmetic products	921:937	The mucilage demonstrates potential for application in cosmetic products, and its commercial use as an ingredient in cosmetics could be a strategic tool for the creation of a new product chain and adding value to the culture of Colocasia0 esculenta.
35361316	2	31	theme	extracted	371:379	arg1	mucilage					381:388	extracted mucilage	371:388	extracted mucilage	371:388	In addition, cosmetic formulations based on extracted mucilage were developed and studied.
35361316	5	32	theme	pseudoplastic	716:728	arg1	behavior					730:737	pseudoplastic behavior	716:737	pseudoplastic behavior	716:737	All cream samples presented pseudoplastic behavior, with a flow behavior index lower than 1, which is a desirable characteristic for cosmetics, as it improves its applicability.
35361316	1	33	theme	thermal	283:289	arg1	analysis					291:298	thermal analysis	283:298	thermal analysis	283:298	Taro mucilage, a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott, was extracted and characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, and proximal composition.
35361316	0	34	from	Application	49:59	arg1	Formulations					73:84	Cosmetic Formulations	64:84	Cosmetic Formulations	64:84	Taro Mucilage: Extraction, Characterization, and Application in Cosmetic Formulations.
35361316	3	35	theme	emulsifying	556:566	arg1	activity					568:575	good emulsifying activity	551:575	good emulsifying activity	551:575	The mucilage presented a semicrystalline structure with high thermal stability, the presence of granules along its surface area, and good emulsifying activity.
35361316	3	36	theme	thermal	479:485	arg1	stability					487:495	high thermal stability	474:495	high thermal stability	474:495	The mucilage presented a semicrystalline structure with high thermal stability, the presence of granules along its surface area, and good emulsifying activity.
35361316	4	37	located	found	620:624	arg2	stability					601:609	High physical-chemical stability	578:609	High physical-chemical stability	578:609	High physical-chemical stability was also found in the mucilage and the cosmetic formulations during storage.
35361316	4	37	located	found	620:624	arg1	mucilage					633:640	the mucilage	629:640	the mucilage	629:640	High physical-chemical stability was also found in the mucilage and the cosmetic formulations during storage.
35361316	4	37	located	found	620:624	arg1	formulations					659:670	the cosmetic formulations	646:670	the cosmetic formulations	646:670	High physical-chemical stability was also found in the mucilage and the cosmetic formulations during storage.
35361316	6	38	theme	cosmetic	921:928	arg1	products					930:937	cosmetic products	921:937	cosmetic products	921:937	The mucilage demonstrates potential for application in cosmetic products, and its commercial use as an ingredient in cosmetics could be a strategic tool for the creation of a new product chain and adding value to the culture of Colocasia0 esculenta.
35361316	5	39	theme	behavior	752:759	arg1	index					761:765	a flow behavior index	745:765	a flow behavior index lower than 1, which is a desirable characteristic for cosmetics	745:829	All cream samples presented pseudoplastic behavior, with a flow behavior index lower than 1, which is a desirable characteristic for cosmetics, as it improves its applicability.
35361316	1	40	theme	present	117:123	arg1	mucilage					92:99	Taro mucilage	87:99	Taro mucilage	87:99	Taro mucilage, a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott, was extracted and characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, and proximal composition.
35361316	1	40	theme	present	117:123	arg1	hydrocolloid					104:115	a hydrocolloid	102:115	a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott	102:173	Taro mucilage, a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott, was extracted and characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, and proximal composition.
35361316	6	41	theme	strategic	1004:1012	arg1	tool					1014:1017	a strategic tool	1002:1017	a strategic tool for the creation of a new product chain and adding value to the culture of Colocasia0 esculenta	1002:1113	The mucilage demonstrates potential for application in cosmetic products, and its commercial use as an ingredient in cosmetics could be a strategic tool for the creation of a new product chain and adding value to the culture of Colocasia0 esculenta.
35361316	6	41	theme	strategic	1004:1012	arg1	use					959:961	its commercial use	944:961	its commercial use as an ingredient in cosmetics	944:991	The mucilage demonstrates potential for application in cosmetic products, and its commercial use as an ingredient in cosmetics could be a strategic tool for the creation of a new product chain and adding value to the culture of Colocasia0 esculenta.
35361316	3	42	theme	high	474:477	arg1	stability					487:495	high thermal stability	474:495	high thermal stability	474:495	The mucilage presented a semicrystalline structure with high thermal stability, the presence of granules along its surface area, and good emulsifying activity.
35361316	4	43	theme	physical-chemical	583:599	arg1	stability					601:609	High physical-chemical stability	578:609	High physical-chemical stability	578:609	High physical-chemical stability was also found in the mucilage and the cosmetic formulations during storage.
35361316	5	44	theme	flow	747:750	arg1	index					761:765	a flow behavior index	745:765	a flow behavior index lower than 1, which is a desirable characteristic for cosmetics	745:829	All cream samples presented pseudoplastic behavior, with a flow behavior index lower than 1, which is a desirable characteristic for cosmetics, as it improves its applicability.
35361316	0	45	dep	Mucilage	5:12	arg1	Characterization					27:42	Characterization	27:42	Characterization	27:42	Taro Mucilage: Extraction, Characterization, and Application in Cosmetic Formulations.
35361316	0	45	dep	Mucilage	5:12	arg1	Application					49:59	Application	49:59	Application	49:59	Taro Mucilage: Extraction, Characterization, and Application in Cosmetic Formulations.
35361316	0	45	dep	Mucilage	5:12	arg1	Extraction					15:24	Extraction	15:24	Extraction	15:24	Taro Mucilage: Extraction, Characterization, and Application in Cosmetic Formulations.
35361316	3	46	theme	semicrystalline	443:457	arg1	structure					459:467	a semicrystalline structure	441:467	a semicrystalline structure	441:467	The mucilage presented a semicrystalline structure with high thermal stability, the presence of granules along its surface area, and good emulsifying activity.
35361316	2	47	theme	cosmetic	340:347	arg1	formulations					349:360	cosmetic formulations	340:360	cosmetic formulations based on extracted mucilage	340:388	In addition, cosmetic formulations based on extracted mucilage were developed and studied.
35361316	4	48	theme	High	578:581	arg1	stability					601:609	High physical-chemical stability	578:609	High physical-chemical stability	578:609	High physical-chemical stability was also found in the mucilage and the cosmetic formulations during storage.
35361316	0	49	from	Extraction	15:24	arg1	Formulations					73:84	Cosmetic Formulations	64:84	Cosmetic Formulations	64:84	Taro Mucilage: Extraction, Characterization, and Application in Cosmetic Formulations.
35361316	6	50	theme	adding	1063:1068	arg1	value					1070:1074	a new product chain and adding value	1039:1074	value	1070:1074	The mucilage demonstrates potential for application in cosmetic products, and its commercial use as an ingredient in cosmetics could be a strategic tool for the creation of a new product chain and adding value to the culture of Colocasia0 esculenta.
35361316	1	51	theme	infrared	211:218	arg1	spectroscopy					220:231	infrared spectroscopy	211:231	infrared spectroscopy	211:231	Taro mucilage, a hydrocolloid present in the rhizome of Colocasia esculenta (L.) Schott, was extracted and characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, and proximal composition.
34123271	4	0	theme	traditional	598:608	arg1	analysis					617:624	traditional glycan analysis	598:624	traditional glycan analysis	598:624	Bacteria produce glycans containing rare deoxy amino sugars refractory to traditional glycan analysis, complicating the study of bacterial glycans and the creation of glycosylation inhibitors.
34123271	10	1	theme	infectious	1494:1503	arg1	disease					1505:1511	infectious disease	1494:1511	infectious disease	1494:1511	Moreover, their selectivity will provide a pathway for the development of novel, narrow-spectrum antibiotics to treat infectious disease.
34123271	11	2	theme	inhibition	1518:1527	arg1	approach					1529:1536	Our inhibition approach	1514:1536	Our inhibition approach	1514:1536	Our inhibition approach is general and will expedite the identification of bacterial glycan biosynthesis inhibitors in a range of systems, expanding the glycochemistry toolkit.
34123271	10	3	dep	novel	1450:1454	arg1	narrow-spectrum					1457:1471	narrow-spectrum	1457:1471	narrow-spectrum	1457:1471	Moreover, their selectivity will provide a pathway for the development of novel, narrow-spectrum antibiotics to treat infectious disease.
34123271	2	4	contain	contains	235:242	arg1	glycocalyx					224:233	The dense cell wall glycocalyx	204:233	The dense cell wall glycocalyx	204:233	The dense cell wall glycocalyx contains distinctive monosaccharides that are absent from human cells, and proper assembly of monosaccharides into higher-order glycans is critical for bacterial fitness and pathogenesis.
34123271	2	4	contain	contains	235:242	arg2	monosaccharides					256:270	distinctive monosaccharides	244:270	distinctive monosaccharides that are absent from human cells	244:303	The dense cell wall glycocalyx contains distinctive monosaccharides that are absent from human cells, and proper assembly of monosaccharides into higher-order glycans is critical for bacterial fitness and pathogenesis.
34123271	9	5	theme	systematic	1280:1289	arg1	study					1291:1295	systematic study	1280:1295	systematic study of bacterial glycosylation enzymes not currently possible with existing tools	1280:1373	These metabolic inhibitors will provide a platform for systematic study of bacterial glycosylation enzymes not currently possible with existing tools.
34123271	2	6	theme	distinctive	244:254	arg1	monosaccharides					256:270	distinctive monosaccharides	244:270	distinctive monosaccharides that are absent from human cells	244:303	The dense cell wall glycocalyx contains distinctive monosaccharides that are absent from human cells, and proper assembly of monosaccharides into higher-order glycans is critical for bacterial fitness and pathogenesis.
34123271	1	7	theme	host	159:162	arg1	colonization					139:150	colonization	139:150	colonization of the host	139:162	The bacterial cell wall is a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion.
34123271	4	8	theme	glycosylation	691:703	arg1	inhibitors					705:714	glycosylation inhibitors	691:714	glycosylation inhibitors	691:714	Bacteria produce glycans containing rare deoxy amino sugars refractory to traditional glycan analysis, complicating the study of bacterial glycans and the creation of glycosylation inhibitors.
34123271	2	9	theme	human	293:297	arg1	cells					299:303	human cells	293:303	human cells	293:303	The dense cell wall glycocalyx contains distinctive monosaccharides that are absent from human cells, and proper assembly of monosaccharides into higher-order glycans is critical for bacterial fitness and pathogenesis.
34123271	1	10	theme	pathogen	165:172	arg1	survival					174:181	pathogen survival	165:181	pathogen survival	165:181	The bacterial cell wall is a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion.
34123271	6	11	theme	pathogenic	1013:1022	arg1	species					1048:1054	pathogenic and symbiotic bacterial species	1013:1054	pathogenic and symbiotic bacterial species	1013:1054	Metabolic inhibitors were assessed for their ability to interfere with glycan biosynthesis and fitness in pathogenic and symbiotic bacterial species.
34123271	4	12	theme	bacterial	653:661	arg1	glycans					663:669	bacterial glycans	653:669	bacterial glycans	653:669	Bacteria produce glycans containing rare deoxy amino sugars refractory to traditional glycan analysis, complicating the study of bacterial glycans and the creation of glycosylation inhibitors.
34123271	7	13	theme	Helicobacter	1137:1148	arg1	pylori					1150:1155	Helicobacter pylori	1137:1155	Helicobacter pylori	1137:1155	Three metabolic inhibitors led to dramatic structural and functional defects in Helicobacter pylori.
34123271	9	14	theme	glycosylation	1310:1322	arg1	enzymes					1324:1330	bacterial glycosylation enzymes	1300:1330	bacterial glycosylation enzymes not currently possible with existing tools	1300:1373	These metabolic inhibitors will provide a platform for systematic study of bacterial glycosylation enzymes not currently possible with existing tools.
34123271	5	15	theme	bacterial	738:746	arg1	function					755:762	bacterial glycan function	738:762	bacterial glycan function	738:762	To ease the study of bacterial glycan function in the absence of detailed structural or enzyme information, we crafted metabolic inhibitors based on rare bacterial monosaccharide scaffolds.
34123271	5	16	theme	metabolic	836:844	arg1	inhibitors					846:855	metabolic inhibitors	836:855	metabolic inhibitors based on rare bacterial monosaccharide scaffolds	836:904	To ease the study of bacterial glycan function in the absence of detailed structural or enzyme information, we crafted metabolic inhibitors based on rare bacterial monosaccharide scaffolds.
34123271	5	17	theme	bacterial	871:879	arg1	scaffolds					896:904	rare bacterial monosaccharide scaffolds	866:904	rare bacterial monosaccharide scaffolds	866:904	To ease the study of bacterial glycan function in the absence of detailed structural or enzyme information, we crafted metabolic inhibitors based on rare bacterial monosaccharide scaffolds.
34123271	9	18	theme	possible	1346:1353	arg1	enzymes					1324:1330	bacterial glycosylation enzymes	1300:1330	bacterial glycosylation enzymes not currently possible with existing tools	1300:1373	These metabolic inhibitors will provide a platform for systematic study of bacterial glycosylation enzymes not currently possible with existing tools.
34123271	1	19	from	survival	174:181	arg1	colonization					139:150	colonization	139:150	colonization of the host	139:162	The bacterial cell wall is a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion.
34123271	11	20	theme	biosynthesis	1606:1617	arg1	inhibitors					1619:1628	bacterial glycan biosynthesis inhibitors	1589:1628	bacterial glycan biosynthesis inhibitors	1589:1628	Our inhibition approach is general and will expedite the identification of bacterial glycan biosynthesis inhibitors in a range of systems, expanding the glycochemistry toolkit.
34123271	7	21	theme	dramatic	1091:1098	arg1	defects					1126:1132	dramatic structural and functional defects	1091:1132	dramatic structural and functional defects in Helicobacter pylori	1091:1155	Three metabolic inhibitors led to dramatic structural and functional defects in Helicobacter pylori.
34123271	9	22	theme	existing	1360:1367	arg1	tools					1369:1373	existing tools	1360:1373	existing tools	1360:1373	These metabolic inhibitors will provide a platform for systematic study of bacterial glycosylation enzymes not currently possible with existing tools.
34123271	1	23	theme	critical	122:129	arg1	role					131:134	its critical role	118:134	its critical role in colonization of the host	118:162	The bacterial cell wall is a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion.
34123271	11	24	theme	bacterial	1589:1597	arg1	inhibitors					1619:1628	bacterial glycan biosynthesis inhibitors	1589:1628	bacterial glycan biosynthesis inhibitors	1589:1628	Our inhibition approach is general and will expedite the identification of bacterial glycan biosynthesis inhibitors in a range of systems, expanding the glycochemistry toolkit.
34123271	2	25	theme	cell	214:217	arg1	glycocalyx					224:233	The dense cell wall glycocalyx	204:233	The dense cell wall glycocalyx	204:233	The dense cell wall glycocalyx contains distinctive monosaccharides that are absent from human cells, and proper assembly of monosaccharides into higher-order glycans is critical for bacterial fitness and pathogenesis.
34123271	5	26	theme	structural	791:800	arg1	absence					771:777	the absence	767:777	the absence of detailed structural or enzyme information	767:822	To ease the study of bacterial glycan function in the absence of detailed structural or enzyme information, we crafted metabolic inhibitors based on rare bacterial monosaccharide scaffolds.
34123271	2	27	from	cells	299:303	arg1	absent					281:286	absent	281:286	absent	281:286	The dense cell wall glycocalyx contains distinctive monosaccharides that are absent from human cells, and proper assembly of monosaccharides into higher-order glycans is critical for bacterial fitness and pathogenesis.
34123271	5	28	theme	function	755:762	arg1	study					729:733	the study	725:733	the study of bacterial glycan function	725:762	To ease the study of bacterial glycan function in the absence of detailed structural or enzyme information, we crafted metabolic inhibitors based on rare bacterial monosaccharide scaffolds.
34123271	1	29	from	role	131:134	arg1	colonization					139:150	colonization	139:150	colonization of the host	139:162	The bacterial cell wall is a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion.
34123271	1	30	theme	cell	69:72	arg1	wall					74:77	The bacterial cell wall	55:77	The bacterial cell wall	55:77	The bacterial cell wall is a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion.
34123271	1	30	theme	cell	69:72	arg1	target					104:109	a quintessential drug target	82:109	a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion	82:201	The bacterial cell wall is a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion.
34123271	6	31	theme	symbiotic	1028:1036	arg1	species					1048:1054	pathogenic and symbiotic bacterial species	1013:1054	pathogenic and symbiotic bacterial species	1013:1054	Metabolic inhibitors were assessed for their ability to interfere with glycan biosynthesis and fitness in pathogenic and symbiotic bacterial species.
34123271	0	32	theme	Metabolic	0:8	arg1	inhibitors					10:19	Metabolic inhibitors	0:19	Metabolic inhibitors of bacterial glycan biosynthesis	0:52	Metabolic inhibitors of bacterial glycan biosynthesis.
34123271	1	33	theme	quintessential	84:97	arg1	wall					74:77	The bacterial cell wall	55:77	The bacterial cell wall	55:77	The bacterial cell wall is a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion.
34123271	1	33	theme	quintessential	84:97	arg1	target					104:109	a quintessential drug target	82:109	a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion	82:201	The bacterial cell wall is a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion.
34123271	4	34	theme	deoxy	565:569	arg1	sugars					577:582	rare deoxy amino sugars	560:582	rare deoxy amino sugars refractory to traditional glycan analysis	560:624	Bacteria produce glycans containing rare deoxy amino sugars refractory to traditional glycan analysis, complicating the study of bacterial glycans and the creation of glycosylation inhibitors.
34123271	10	35	theme	antibiotics	1473:1483	arg1	development					1435:1445	the development	1431:1445	the development of novel, narrow-spectrum antibiotics to treat infectious disease	1431:1511	Moreover, their selectivity will provide a pathway for the development of novel, narrow-spectrum antibiotics to treat infectious disease.
34123271	6	36	theme	Metabolic	907:915	arg1	inhibitors					917:926	Metabolic inhibitors	907:926	Metabolic inhibitors	907:926	Metabolic inhibitors were assessed for their ability to interfere with glycan biosynthesis and fitness in pathogenic and symbiotic bacterial species.
34123271	3	37	theme	systematic	436:445	arg1	study					447:451	systematic study	436:451	systematic study	436:451	However, the systematic study and inhibition of bacterial glycosylation enzymes remains challenging.
34123271	0	38	theme	glycan	34:39	arg1	biosynthesis					41:52	bacterial glycan biosynthesis	24:52	bacterial glycan biosynthesis	24:52	Metabolic inhibitors of bacterial glycan biosynthesis.
34123271	5	39	theme	enzyme	805:810	arg1	information					812:822	detailed structural or enzyme information	782:822	information	812:822	To ease the study of bacterial glycan function in the absence of detailed structural or enzyme information, we crafted metabolic inhibitors based on rare bacterial monosaccharide scaffolds.
34123271	3	40	theme	glycosylation	481:493	arg1	enzymes					495:501	bacterial glycosylation enzymes	471:501	bacterial glycosylation enzymes	471:501	However, the systematic study and inhibition of bacterial glycosylation enzymes remains challenging.
34123271	3	41	dep	study	447:451	arg1	the					432:434	the	432:434	the	432:434	However, the systematic study and inhibition of bacterial glycosylation enzymes remains challenging.
34123271	9	42	theme	metabolic	1231:1239	arg1	inhibitors					1241:1250	These metabolic inhibitors	1225:1250	These metabolic inhibitors	1225:1250	These metabolic inhibitors will provide a platform for systematic study of bacterial glycosylation enzymes not currently possible with existing tools.
34123271	2	43	theme	higher-order	350:361	arg1	glycans					363:369	higher-order glycans	350:369	higher-order glycans	350:369	The dense cell wall glycocalyx contains distinctive monosaccharides that are absent from human cells, and proper assembly of monosaccharides into higher-order glycans is critical for bacterial fitness and pathogenesis.
34123271	2	44	theme	monosaccharides	329:343	arg1	assembly					317:324	proper assembly	310:324	proper assembly of monosaccharides into higher-order glycans	310:369	The dense cell wall glycocalyx contains distinctive monosaccharides that are absent from human cells, and proper assembly of monosaccharides into higher-order glycans is critical for bacterial fitness and pathogenesis.
34123271	7	45	from	defects	1126:1132	arg1	pylori					1150:1155	Helicobacter pylori	1137:1155	Helicobacter pylori	1137:1155	Three metabolic inhibitors led to dramatic structural and functional defects in Helicobacter pylori.
34123271	4	46	contain	containing	549:558	arg2	sugars					577:582	rare deoxy amino sugars	560:582	rare deoxy amino sugars refractory to traditional glycan analysis	560:624	Bacteria produce glycans containing rare deoxy amino sugars refractory to traditional glycan analysis, complicating the study of bacterial glycans and the creation of glycosylation inhibitors.
34123271	4	46	contain	containing	549:558	arg1	glycans					541:547	glycans	541:547	glycans containing rare deoxy amino sugars refractory to traditional glycan analysis	541:624	Bacteria produce glycans containing rare deoxy amino sugars refractory to traditional glycan analysis, complicating the study of bacterial glycans and the creation of glycosylation inhibitors.
34123271	4	47	theme	glycan	610:615	arg1	analysis					617:624	traditional glycan analysis	598:624	traditional glycan analysis	598:624	Bacteria produce glycans containing rare deoxy amino sugars refractory to traditional glycan analysis, complicating the study of bacterial glycans and the creation of glycosylation inhibitors.
34123271	11	48	theme	systems	1644:1650	arg1	range					1635:1639	a range	1633:1639	a range of systems, expanding the glycochemistry toolkit	1633:1688	Our inhibition approach is general and will expedite the identification of bacterial glycan biosynthesis inhibitors in a range of systems, expanding the glycochemistry toolkit.
34123271	5	49	theme	monosaccharide	881:894	arg1	scaffolds					896:904	rare bacterial monosaccharide scaffolds	866:904	rare bacterial monosaccharide scaffolds	866:904	To ease the study of bacterial glycan function in the absence of detailed structural or enzyme information, we crafted metabolic inhibitors based on rare bacterial monosaccharide scaffolds.
34123271	9	50	theme	bacterial	1300:1308	arg1	enzymes					1324:1330	bacterial glycosylation enzymes	1300:1330	bacterial glycosylation enzymes not currently possible with existing tools	1300:1373	These metabolic inhibitors will provide a platform for systematic study of bacterial glycosylation enzymes not currently possible with existing tools.
34123271	2	51	theme	proper	310:315	arg1	assembly					317:324	proper assembly	310:324	proper assembly of monosaccharides into higher-order glycans	310:369	The dense cell wall glycocalyx contains distinctive monosaccharides that are absent from human cells, and proper assembly of monosaccharides into higher-order glycans is critical for bacterial fitness and pathogenesis.
34123271	7	52	theme	functional	1115:1124	arg1	defects					1126:1132	dramatic structural and functional defects	1091:1132	dramatic structural and functional defects in Helicobacter pylori	1091:1155	Three metabolic inhibitors led to dramatic structural and functional defects in Helicobacter pylori.
34123271	9	53	theme	enzymes	1324:1330	arg1	study					1291:1295	systematic study	1280:1295	systematic study of bacterial glycosylation enzymes not currently possible with existing tools	1280:1373	These metabolic inhibitors will provide a platform for systematic study of bacterial glycosylation enzymes not currently possible with existing tools.
34123271	2	54	theme	dense	208:212	arg1	glycocalyx					224:233	The dense cell wall glycocalyx	204:233	The dense cell wall glycocalyx	204:233	The dense cell wall glycocalyx contains distinctive monosaccharides that are absent from human cells, and proper assembly of monosaccharides into higher-order glycans is critical for bacterial fitness and pathogenesis.
34123271	5	55	theme	rare	866:869	arg1	scaffolds					896:904	rare bacterial monosaccharide scaffolds	866:904	rare bacterial monosaccharide scaffolds	866:904	To ease the study of bacterial glycan function in the absence of detailed structural or enzyme information, we crafted metabolic inhibitors based on rare bacterial monosaccharide scaffolds.
34123271	4	56	theme	glycans	663:669	arg1	creation					679:686	the creation	675:686	the creation of glycosylation inhibitors	675:714	Bacteria produce glycans containing rare deoxy amino sugars refractory to traditional glycan analysis, complicating the study of bacterial glycans and the creation of glycosylation inhibitors.
34123271	4	56	theme	glycans	663:669	arg1	study					644:648	the study	640:648	the study of bacterial glycans	640:669	Bacteria produce glycans containing rare deoxy amino sugars refractory to traditional glycan analysis, complicating the study of bacterial glycans and the creation of glycosylation inhibitors.
34123271	7	57	theme	structural	1100:1109	arg1	defects					1126:1132	dramatic structural and functional defects	1091:1132	dramatic structural and functional defects in Helicobacter pylori	1091:1155	Three metabolic inhibitors led to dramatic structural and functional defects in Helicobacter pylori.
34123271	1	58	theme	due	111:113	arg1	wall					74:77	The bacterial cell wall	55:77	The bacterial cell wall	55:77	The bacterial cell wall is a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion.
34123271	1	58	theme	due	111:113	arg1	target					104:109	a quintessential drug target	82:109	a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion	82:201	The bacterial cell wall is a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion.
34123271	6	59	theme	glycan	978:983	arg1	biosynthesis					985:996	biosynthesis	985:996	biosynthesis	985:996	Metabolic inhibitors were assessed for their ability to interfere with glycan biosynthesis and fitness in pathogenic and symbiotic bacterial species.
34123271	11	60	theme	glycan	1599:1604	arg1	inhibitors					1619:1628	bacterial glycan biosynthesis inhibitors	1589:1628	bacterial glycan biosynthesis inhibitors	1589:1628	Our inhibition approach is general and will expedite the identification of bacterial glycan biosynthesis inhibitors in a range of systems, expanding the glycochemistry toolkit.
34123271	11	61	theme	glycochemistry	1667:1680	arg1	toolkit					1682:1688	the glycochemistry toolkit	1663:1688	the glycochemistry toolkit	1663:1688	Our inhibition approach is general and will expedite the identification of bacterial glycan biosynthesis inhibitors in a range of systems, expanding the glycochemistry toolkit.
34123271	1	62	from	evasion	195:201	arg1	colonization					139:150	colonization	139:150	colonization of the host	139:162	The bacterial cell wall is a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion.
34123271	7	63	theme	metabolic	1063:1071	arg1	inhibitors					1073:1082	Three metabolic inhibitors	1057:1082	Three metabolic inhibitors	1057:1082	Three metabolic inhibitors led to dramatic structural and functional defects in Helicobacter pylori.
34123271	2	64	theme	wall	219:222	arg1	glycocalyx					224:233	The dense cell wall glycocalyx	204:233	The dense cell wall glycocalyx	204:233	The dense cell wall glycocalyx contains distinctive monosaccharides that are absent from human cells, and proper assembly of monosaccharides into higher-order glycans is critical for bacterial fitness and pathogenesis.
34123271	4	65	theme	inhibitors	705:714	arg1	creation					679:686	the creation	675:686	the creation of glycosylation inhibitors	675:714	Bacteria produce glycans containing rare deoxy amino sugars refractory to traditional glycan analysis, complicating the study of bacterial glycans and the creation of glycosylation inhibitors.
34123271	4	65	theme	inhibitors	705:714	arg1	study					644:648	the study	640:648	the study of bacterial glycans	640:669	Bacteria produce glycans containing rare deoxy amino sugars refractory to traditional glycan analysis, complicating the study of bacterial glycans and the creation of glycosylation inhibitors.
34123271	11	66	theme	inhibitors	1619:1628	arg1	identification					1571:1584	the identification	1567:1584	the identification of bacterial glycan biosynthesis inhibitors	1567:1628	Our inhibition approach is general and will expedite the identification of bacterial glycan biosynthesis inhibitors in a range of systems, expanding the glycochemistry toolkit.
34123271	5	67	theme	glycan	748:753	arg1	function					755:762	bacterial glycan function	738:762	bacterial glycan function	738:762	To ease the study of bacterial glycan function in the absence of detailed structural or enzyme information, we crafted metabolic inhibitors based on rare bacterial monosaccharide scaffolds.
34123271	1	68	theme	bacterial	59:67	arg1	wall					74:77	The bacterial cell wall	55:77	The bacterial cell wall	55:77	The bacterial cell wall is a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion.
34123271	1	68	theme	bacterial	59:67	arg1	target					104:109	a quintessential drug target	82:109	a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion	82:201	The bacterial cell wall is a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion.
34123271	6	69	theme	bacterial	1038:1046	arg1	species					1048:1054	pathogenic and symbiotic bacterial species	1013:1054	pathogenic and symbiotic bacterial species	1013:1054	Metabolic inhibitors were assessed for their ability to interfere with glycan biosynthesis and fitness in pathogenic and symbiotic bacterial species.
34123271	9	70	with	possible	1346:1353	arg1	tools					1369:1373	existing tools	1360:1373	existing tools	1360:1373	These metabolic inhibitors will provide a platform for systematic study of bacterial glycosylation enzymes not currently possible with existing tools.
34123271	4	71	theme	amino	571:575	arg1	sugars					577:582	rare deoxy amino sugars	560:582	rare deoxy amino sugars refractory to traditional glycan analysis	560:624	Bacteria produce glycans containing rare deoxy amino sugars refractory to traditional glycan analysis, complicating the study of bacterial glycans and the creation of glycosylation inhibitors.
34123271	0	72	theme	bacterial	24:32	arg1	biosynthesis					41:52	bacterial glycan biosynthesis	24:52	bacterial glycan biosynthesis	24:52	Metabolic inhibitors of bacterial glycan biosynthesis.
34123271	1	73	theme	drug	99:102	arg1	wall					74:77	The bacterial cell wall	55:77	The bacterial cell wall	55:77	The bacterial cell wall is a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion.
34123271	1	73	theme	drug	99:102	arg1	target					104:109	a quintessential drug target	82:109	a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion	82:201	The bacterial cell wall is a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion.
34123271	4	74	theme	refractory	584:593	arg1	sugars					577:582	rare deoxy amino sugars	560:582	rare deoxy amino sugars refractory to traditional glycan analysis	560:624	Bacteria produce glycans containing rare deoxy amino sugars refractory to traditional glycan analysis, complicating the study of bacterial glycans and the creation of glycosylation inhibitors.
34123271	6	75	from	fitness	1002:1008	arg1	species					1048:1054	pathogenic and symbiotic bacterial species	1013:1054	pathogenic and symbiotic bacterial species	1013:1054	Metabolic inhibitors were assessed for their ability to interfere with glycan biosynthesis and fitness in pathogenic and symbiotic bacterial species.
34123271	0	76	theme	biosynthesis	41:52	arg1	inhibitors					10:19	Metabolic inhibitors	0:19	Metabolic inhibitors of bacterial glycan biosynthesis	0:52	Metabolic inhibitors of bacterial glycan biosynthesis.
34123271	2	77	theme	bacterial	387:395	arg1	fitness					397:403	bacterial fitness	387:403	bacterial fitness	387:403	The dense cell wall glycocalyx contains distinctive monosaccharides that are absent from human cells, and proper assembly of monosaccharides into higher-order glycans is critical for bacterial fitness and pathogenesis.
34123271	5	78	theme	information	812:822	arg1	absence					771:777	the absence	767:777	the absence of detailed structural or enzyme information	767:822	To ease the study of bacterial glycan function in the absence of detailed structural or enzyme information, we crafted metabolic inhibitors based on rare bacterial monosaccharide scaffolds.
34123271	4	79	theme	rare	560:563	arg1	sugars					577:582	rare deoxy amino sugars	560:582	rare deoxy amino sugars refractory to traditional glycan analysis	560:624	Bacteria produce glycans containing rare deoxy amino sugars refractory to traditional glycan analysis, complicating the study of bacterial glycans and the creation of glycosylation inhibitors.
34123271	3	80	theme	enzymes	495:501	arg1	study					447:451	systematic study	436:451	systematic study	436:451	However, the systematic study and inhibition of bacterial glycosylation enzymes remains challenging.
34123271	3	80	theme	enzymes	495:501	arg1	inhibition					457:466	inhibition	457:466	inhibition	457:466	However, the systematic study and inhibition of bacterial glycosylation enzymes remains challenging.
34123271	6	81	from	biosynthesis	985:996	arg1	species					1048:1054	pathogenic and symbiotic bacterial species	1013:1054	pathogenic and symbiotic bacterial species	1013:1054	Metabolic inhibitors were assessed for their ability to interfere with glycan biosynthesis and fitness in pathogenic and symbiotic bacterial species.
34123271	3	82	theme	bacterial	471:479	arg1	enzymes					495:501	bacterial glycosylation enzymes	471:501	bacterial glycosylation enzymes	471:501	However, the systematic study and inhibition of bacterial glycosylation enzymes remains challenging.
34123271	5	83	from	ease	720:723	arg1	absence					771:777	the absence	767:777	the absence of detailed structural or enzyme information	767:822	To ease the study of bacterial glycan function in the absence of detailed structural or enzyme information, we crafted metabolic inhibitors based on rare bacterial monosaccharide scaffolds.
34123271	8	84	theme	bacteria-selective	1198:1215	arg1	manner					1217:1222	a bacteria-selective manner	1196:1222	a bacteria-selective manner	1196:1222	Strikingly, these inhibitors acted in a bacteria-selective manner.
34123271	1	85	theme	immune	188:193	arg1	evasion					195:201	immune evasion	188:201	immune evasion	188:201	The bacterial cell wall is a quintessential drug target due to its critical role in colonization of the host, pathogen survival, and immune evasion.
34123271	10	86	theme	novel	1450:1454	arg1	antibiotics					1473:1483	novel, narrow-spectrum antibiotics	1450:1483	novel, narrow-spectrum antibiotics	1450:1483	Moreover, their selectivity will provide a pathway for the development of novel, narrow-spectrum antibiotics to treat infectious disease.
34773587	8	0	theme	cheese	1418:1423	arg1	substrates					1395:1404	cost-effective substrates	1380:1404	cost-effective substrates	1380:1404	Henceforth, the ability of Halomonas strains to use cost-effective substrates, especially cheese whey, is a promising approach for the production of EPS with distinct physicochemical properties suitable for various applications.
34773587	8	0	theme	cheese	1418:1423	arg1	whey					1425:1428	cheese whey	1418:1428	cheese whey	1418:1428	Henceforth, the ability of Halomonas strains to use cost-effective substrates, especially cheese whey, is a promising approach for the production of EPS with distinct physicochemical properties suitable for various applications.
34773587	8	1	theme	Halomonas	1355:1363	arg1	strains					1365:1371	Halomonas strains	1355:1371	Halomonas strains	1355:1371	Henceforth, the ability of Halomonas strains to use cost-effective substrates, especially cheese whey, is a promising approach for the production of EPS with distinct physicochemical properties suitable for various applications.
34773587	1	2	theme	annually	178:185	arg1	worldwide					187:195	annually worldwide	178:195	annually worldwide	178:195	Large quantities of waste biomass are generated annually worldwide by many industries and are vastly underutilized.
34773587	2	3	contain	contain	268:274	arg2	sugars					276:281	sugars	276:281	sugars	276:281	However, these wastes contain sugars and other dissolved organic matter and therefore can be exploited to produce microbial biopolymers.
34773587	2	3	contain	contain	268:274	arg2	matter					311:316	other dissolved organic matter	287:316	other dissolved organic matter	287:316	However, these wastes contain sugars and other dissolved organic matter and therefore can be exploited to produce microbial biopolymers.
34773587	2	3	contain	contain	268:274	arg1	wastes					261:266	these wastes	255:266	these wastes	255:266	However, these wastes contain sugars and other dissolved organic matter and therefore can be exploited to produce microbial biopolymers.
34773587	0	4	theme	Halomonas	111:119	arg1	strains					121:127	selected Halomonas strains	102:127	selected Halomonas strains	102:127	Sustainable use of agro-industrial wastes as potential feedstocks for exopolysaccharide production by selected Halomonas strains.
34773587	3	5	theme	grape	686:690	arg1	pomace					692:697	grape pomace	686:697	grape pomace	686:697	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	5	theme	grape	686:690	arg1	exopolysaccharides					583:600	exopolysaccharides	583:600	exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol	583:711	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	6	theme	selected	403:410	arg1	K1					463:464	Halomonas caseinilytica K1	439:464	Halomonas caseinilytica K1	439:464	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	6	theme	selected	403:410	arg1	strains					422:428	four selected Halomonas strains	398:428	four selected Halomonas strains	398:428	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	6	theme	selected	403:410	arg1	S4					539:540	Halomonas halophila S4	519:540	Halomonas halophila S4	519:540	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	6	theme	selected	403:410	arg1	S3					511:512	Halomonas smyrnensis S3	490:512	Halomonas smyrnensis S3	490:512	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	6	theme	selected	403:410	arg1	K4					486:487	Halomonas elongata K4	467:487	Halomonas elongata K4	467:487	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	0	7	theme	selected	102:109	arg1	strains					121:127	selected Halomonas strains	102:127	selected Halomonas strains	102:127	Sustainable use of agro-industrial wastes as potential feedstocks for exopolysaccharide production by selected Halomonas strains.
34773587	8	8	theme	promising	1436:1444	arg1	ability					1344:1350	the ability	1340:1350	the ability of Halomonas strains to use cost-effective substrates, especially cheese whey,	1340:1429	Henceforth, the ability of Halomonas strains to use cost-effective substrates, especially cheese whey, is a promising approach for the production of EPS with distinct physicochemical properties suitable for various applications.
34773587	8	8	theme	promising	1436:1444	arg1	approach					1446:1453	a promising approach	1434:1453	a promising approach for the production of EPS with distinct physicochemical properties suitable for various applications	1434:1554	Henceforth, the ability of Halomonas strains to use cost-effective substrates, especially cheese whey, is a promising approach for the production of EPS with distinct physicochemical properties suitable for various applications.
34773587	3	9	dep	Halomonas	490:498	arg1	smyrnensis					500:509	smyrnensis	500:509	smyrnensis	500:509	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	2	10	theme	organic	303:309	arg1	matter					311:316	other dissolved organic matter	287:316	other dissolved organic matter	287:316	However, these wastes contain sugars and other dissolved organic matter and therefore can be exploited to produce microbial biopolymers.
34773587	3	11	theme	Halomonas	490:498	arg1	strains					422:428	four selected Halomonas strains	398:428	four selected Halomonas strains	398:428	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	11	theme	Halomonas	490:498	arg1	S3					511:512	Halomonas smyrnensis S3	490:512	Halomonas smyrnensis S3	490:512	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	2	12	theme	microbial	360:368	arg1	biopolymers					370:380	microbial biopolymers	360:380	microbial biopolymers	360:380	However, these wastes contain sugars and other dissolved organic matter and therefore can be exploited to produce microbial biopolymers.
34773587	2	13	theme	dissolved	293:301	arg1	matter					311:316	other dissolved organic matter	287:316	other dissolved organic matter	287:316	However, these wastes contain sugars and other dissolved organic matter and therefore can be exploited to produce microbial biopolymers.
34773587	7	14	theme	galacturonic	1246:1257	arg1	acid					1259:1262	galacturonic acid	1246:1262	galacturonic acid	1246:1262	The highest EPS concentration (446 mg/L) was obtained for H. caseinilytica K1 grown on cheese whey that produced an EPS composed mostly of galactose, rhamnose, glucose, and mannose, with lower contents of galacturonic acid, ribose, and arabinose and with a molecular weight of 54.5 kDa.
34773587	3	15	dep	exopolysaccharides	583:600	arg1	pomace					692:697	grape pomace	686:697	grape pomace	686:697	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	15	dep	exopolysaccharides	583:600	arg1	glycerol					704:711	glycerol	704:711	glycerol	704:711	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	15	dep	exopolysaccharides	583:600	arg1	exopolysaccharides					583:600	exopolysaccharides	583:600	exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol	583:711	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	15	dep	exopolysaccharides	583:600	arg1	whey					680:683	cheese whey	673:683	cheese whey	673:683	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	6	16	theme	molecular	956:964	arg1	weight					966:971	the average molecular weight	944:971	the average molecular weight of the synthesized EPS	944:994	Similarly, the average molecular weight of the synthesized EPS was affected, ranging from 54.5 to 4480 kDa.
34773587	2	17	theme	other	287:291	arg1	matter					311:316	other dissolved organic matter	287:316	other dissolved organic matter	287:316	However, these wastes contain sugars and other dissolved organic matter and therefore can be exploited to produce microbial biopolymers.
34773587	7	18	theme	ribose	1265:1270	arg1	contents					1234:1241	lower contents	1228:1241	lower contents of galacturonic acid, ribose, and arabinose	1228:1285	The highest EPS concentration (446 mg/L) was obtained for H. caseinilytica K1 grown on cheese whey that produced an EPS composed mostly of galactose, rhamnose, glucose, and mannose, with lower contents of galacturonic acid, ribose, and arabinose and with a molecular weight of 54.5 kDa.
34773587	7	19	theme	cheese	1128:1133	arg1	whey					1135:1138	cheese whey	1128:1138	cheese whey that produced an EPS composed mostly of galactose, rhamnose, glucose, and mannose, with lower contents of galacturonic acid, ribose, and arabinose and with a molecular weight of 54.5 kDa	1128:1325	The highest EPS concentration (446 mg/L) was obtained for H. caseinilytica K1 grown on cheese whey that produced an EPS composed mostly of galactose, rhamnose, glucose, and mannose, with lower contents of galacturonic acid, ribose, and arabinose and with a molecular weight of 54.5 kDa.
34773587	7	20	theme	highest	1045:1051	arg1	concentration					1057:1069	The highest EPS concentration	1041:1069	The highest EPS concentration (446 mg/L)	1041:1080	The highest EPS concentration (446 mg/L) was obtained for H. caseinilytica K1 grown on cheese whey that produced an EPS composed mostly of galactose, rhamnose, glucose, and mannose, with lower contents of galacturonic acid, ribose, and arabinose and with a molecular weight of 54.5 kDa.
34773587	7	20	theme	highest	1045:1051	arg1	446 mg/L					1072:1079	446 mg/L	1072:1079	446 mg/L	1072:1079	The highest EPS concentration (446 mg/L) was obtained for H. caseinilytica K1 grown on cheese whey that produced an EPS composed mostly of galactose, rhamnose, glucose, and mannose, with lower contents of galacturonic acid, ribose, and arabinose and with a molecular weight of 54.5 kDa.
34773587	6	21	dep	4480 kDa	1031:1038	arg1	to					1028:1029	to	1028:1029	to	1028:1029	Similarly, the average molecular weight of the synthesized EPS was affected, ranging from 54.5 to 4480 kDa.
34773587	8	22	theme	various	1535:1541	arg1	applications					1543:1554	various applications	1535:1554	various applications	1535:1554	Henceforth, the ability of Halomonas strains to use cost-effective substrates, especially cheese whey, is a promising approach for the production of EPS with distinct physicochemical properties suitable for various applications.
34773587	1	23	theme	many	200:203	arg1	industries					205:214	many industries	200:214	many industries	200:214	Large quantities of waste biomass are generated annually worldwide by many industries and are vastly underutilized.
34773587	0	24	theme	Sustainable	0:10	arg1	use					12:14	Sustainable use	0:14	Sustainable use of agro-industrial	0:33	Sustainable use of agro-industrial wastes as potential feedstocks for exopolysaccharide production by selected Halomonas strains.
34773587	6	25	theme	average	948:954	arg1	weight					966:971	the average molecular weight	944:971	the average molecular weight of the synthesized EPS	944:994	Similarly, the average molecular weight of the synthesized EPS was affected, ranging from 54.5 to 4480 kDa.
34773587	7	26	theme	EPS	1053:1055	arg1	concentration					1057:1069	The highest EPS concentration	1041:1069	The highest EPS concentration (446 mg/L)	1041:1080	The highest EPS concentration (446 mg/L) was obtained for H. caseinilytica K1 grown on cheese whey that produced an EPS composed mostly of galactose, rhamnose, glucose, and mannose, with lower contents of galacturonic acid, ribose, and arabinose and with a molecular weight of 54.5 kDa.
34773587	7	26	theme	EPS	1053:1055	arg1	446 mg/L					1072:1079	446 mg/L	1072:1079	446 mg/L	1072:1079	The highest EPS concentration (446 mg/L) was obtained for H. caseinilytica K1 grown on cheese whey that produced an EPS composed mostly of galactose, rhamnose, glucose, and mannose, with lower contents of galacturonic acid, ribose, and arabinose and with a molecular weight of 54.5 kDa.
34773587	3	27	theme	Halomonas	439:447	arg1	strains					422:428	four selected Halomonas strains	398:428	four selected Halomonas strains	398:428	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	27	theme	Halomonas	439:447	arg1	K1					463:464	Halomonas caseinilytica K1	439:464	Halomonas caseinilytica K1	439:464	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	0	28	theme	agro-industrial	19:33	arg1	use					12:14	Sustainable use	0:14	Sustainable use of agro-industrial	0:33	Sustainable use of agro-industrial wastes as potential feedstocks for exopolysaccharide production by selected Halomonas strains.
34773587	5	29	theme	molar	906:910	arg1	ratio					912:916	their relative molar ratio	891:916	their relative molar ratio	891:916	Glucose, mannose, galactose, and rhamnose were the predominant monomers, but their relative molar ratio was different.
34773587	3	30	dep	Halomonas	519:527	arg1	halophila					529:537	halophila	529:537	halophila	529:537	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	8	31	with	EPS	1477:1479	arg1	properties					1511:1520	distinct physicochemical properties	1486:1520	distinct physicochemical properties suitable for various applications	1486:1554	Henceforth, the ability of Halomonas strains to use cost-effective substrates, especially cheese whey, is a promising approach for the production of EPS with distinct physicochemical properties suitable for various applications.
34773587	8	32	theme	EPS	1477:1479	arg1	production					1463:1472	the production	1459:1472	the production of EPS with distinct physicochemical properties suitable for various applications	1459:1554	Henceforth, the ability of Halomonas strains to use cost-effective substrates, especially cheese whey, is a promising approach for the production of EPS with distinct physicochemical properties suitable for various applications.
34773587	4	33	theme	EPS	774:776	arg1	yield					734:738	yield	734:738	yield	734:738	Interestingly, both yield and monosaccharide composition of EPS were affected by the carbon source.
34773587	4	33	theme	EPS	774:776	arg1	composition					759:769	monosaccharide composition	744:769	monosaccharide composition of EPS	744:776	Interestingly, both yield and monosaccharide composition of EPS were affected by the carbon source.
34773587	3	34	theme	sole	653:656	arg1	source					665:670	the sole carbon source	649:670	the sole carbon source	649:670	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	34	theme	sole	653:656	arg1	wastes					639:644	low-cost agro-industrial wastes	614:644	low-cost agro-industrial wastes	614:644	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	7	35	theme	54.5 kDa	1318:1325	arg1	weight					1308:1313	a molecular weight	1296:1313	a molecular weight of 54.5 kDa	1296:1325	The highest EPS concentration (446 mg/L) was obtained for H. caseinilytica K1 grown on cheese whey that produced an EPS composed mostly of galactose, rhamnose, glucose, and mannose, with lower contents of galacturonic acid, ribose, and arabinose and with a molecular weight of 54.5 kDa.
34773587	3	36	theme	Halomonas	519:527	arg1	strains					422:428	four selected Halomonas strains	398:428	four selected Halomonas strains	398:428	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	36	theme	Halomonas	519:527	arg1	S4					539:540	Halomonas halophila S4	519:540	Halomonas halophila S4	519:540	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	37	theme	carbon	658:663	arg1	source					665:670	the sole carbon source	649:670	the sole carbon source	649:670	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	37	theme	carbon	658:663	arg1	wastes					639:644	low-cost agro-industrial wastes	614:644	low-cost agro-industrial wastes	614:644	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	7	38	theme	molecular	1298:1306	arg1	weight					1308:1313	a molecular weight	1296:1313	a molecular weight of 54.5 kDa	1296:1325	The highest EPS concentration (446 mg/L) was obtained for H. caseinilytica K1 grown on cheese whey that produced an EPS composed mostly of galactose, rhamnose, glucose, and mannose, with lower contents of galacturonic acid, ribose, and arabinose and with a molecular weight of 54.5 kDa.
34773587	7	39	theme	acid	1259:1262	arg1	contents					1234:1241	lower contents	1228:1241	lower contents of galacturonic acid, ribose, and arabinose	1228:1285	The highest EPS concentration (446 mg/L) was obtained for H. caseinilytica K1 grown on cheese whey that produced an EPS composed mostly of galactose, rhamnose, glucose, and mannose, with lower contents of galacturonic acid, ribose, and arabinose and with a molecular weight of 54.5 kDa.
34773587	8	40	theme	distinct	1486:1493	arg1	properties					1511:1520	distinct physicochemical properties	1486:1520	distinct physicochemical properties suitable for various applications	1486:1554	Henceforth, the ability of Halomonas strains to use cost-effective substrates, especially cheese whey, is a promising approach for the production of EPS with distinct physicochemical properties suitable for various applications.
34773587	4	41	theme	monosaccharide	744:757	arg1	composition					759:769	monosaccharide composition	744:769	monosaccharide composition of EPS	744:776	Interestingly, both yield and monosaccharide composition of EPS were affected by the carbon source.
34773587	3	42	dep	Halomonas	439:447	arg1	caseinilytica					449:461	caseinilytica	449:461	caseinilytica	449:461	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	43	theme	Halomonas	467:475	arg1	strains					422:428	four selected Halomonas strains	398:428	four selected Halomonas strains	398:428	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	43	theme	Halomonas	467:475	arg1	K4					486:487	Halomonas elongata K4	467:487	Halomonas elongata K4	467:487	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	44	theme	Halomonas	412:420	arg1	K1					463:464	Halomonas caseinilytica K1	439:464	Halomonas caseinilytica K1	439:464	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	44	theme	Halomonas	412:420	arg1	strains					422:428	four selected Halomonas strains	398:428	four selected Halomonas strains	398:428	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	44	theme	Halomonas	412:420	arg1	S4					539:540	Halomonas halophila S4	519:540	Halomonas halophila S4	519:540	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	44	theme	Halomonas	412:420	arg1	S3					511:512	Halomonas smyrnensis S3	490:512	Halomonas smyrnensis S3	490:512	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	44	theme	Halomonas	412:420	arg1	K4					486:487	Halomonas elongata K4	467:487	Halomonas elongata K4	467:487	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	45	theme	exopolysaccharides	583:600	arg1	production					569:578	the production	565:578	the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol	565:711	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	1	46	theme	Large	130:134	arg1	quantities					136:145	Large quantities	130:145	Large quantities of waste biomass	130:162	Large quantities of waste biomass are generated annually worldwide by many industries and are vastly underutilized.
34773587	0	47	theme	potential	45:53	arg1	feedstocks					55:64	potential feedstocks	45:64	potential feedstocks for exopolysaccharide production by selected Halomonas strains	45:127	Sustainable use of agro-industrial wastes as potential feedstocks for exopolysaccharide production by selected Halomonas strains.
34773587	3	48	theme	cheese	673:678	arg1	exopolysaccharides					583:600	exopolysaccharides	583:600	exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol	583:711	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	48	theme	cheese	673:678	arg1	whey					680:683	cheese whey	673:683	cheese whey	673:683	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	8	49	theme	cost-effective	1380:1393	arg1	substrates					1395:1404	cost-effective substrates	1380:1404	cost-effective substrates	1380:1404	Henceforth, the ability of Halomonas strains to use cost-effective substrates, especially cheese whey, is a promising approach for the production of EPS with distinct physicochemical properties suitable for various applications.
34773587	8	49	theme	cost-effective	1380:1393	arg1	whey					1425:1428	cheese whey	1418:1428	cheese whey	1418:1428	Henceforth, the ability of Halomonas strains to use cost-effective substrates, especially cheese whey, is a promising approach for the production of EPS with distinct physicochemical properties suitable for various applications.
34773587	8	50	theme	physicochemical	1495:1509	arg1	properties					1511:1520	distinct physicochemical properties	1486:1520	distinct physicochemical properties suitable for various applications	1486:1554	Henceforth, the ability of Halomonas strains to use cost-effective substrates, especially cheese whey, is a promising approach for the production of EPS with distinct physicochemical properties suitable for various applications.
34773587	7	51	theme	arabinose	1277:1285	arg1	contents					1234:1241	lower contents	1228:1241	lower contents of galacturonic acid, ribose, and arabinose	1228:1285	The highest EPS concentration (446 mg/L) was obtained for H. caseinilytica K1 grown on cheese whey that produced an EPS composed mostly of galactose, rhamnose, glucose, and mannose, with lower contents of galacturonic acid, ribose, and arabinose and with a molecular weight of 54.5 kDa.
34773587	3	52	theme	agro-industrial	623:637	arg1	source					665:670	the sole carbon source	649:670	the sole carbon source	649:670	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	52	theme	agro-industrial	623:637	arg1	wastes					639:644	low-cost agro-industrial wastes	614:644	low-cost agro-industrial wastes	614:644	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	4	53	theme	carbon	799:804	arg1	source					806:811	the carbon source	795:811	the carbon source	795:811	Interestingly, both yield and monosaccharide composition of EPS were affected by the carbon source.
34773587	5	54	theme	relative	897:904	arg1	ratio					912:916	their relative molar ratio	891:916	their relative molar ratio	891:916	Glucose, mannose, galactose, and rhamnose were the predominant monomers, but their relative molar ratio was different.
34773587	8	55	theme	strains	1365:1371	arg1	approach					1446:1453	a promising approach	1434:1453	a promising approach for the production of EPS with distinct physicochemical properties suitable for various applications	1434:1554	Henceforth, the ability of Halomonas strains to use cost-effective substrates, especially cheese whey, is a promising approach for the production of EPS with distinct physicochemical properties suitable for various applications.
34773587	8	55	theme	strains	1365:1371	arg1	ability					1344:1350	the ability	1340:1350	the ability of Halomonas strains to use cost-effective substrates, especially cheese whey,	1340:1429	Henceforth, the ability of Halomonas strains to use cost-effective substrates, especially cheese whey, is a promising approach for the production of EPS with distinct physicochemical properties suitable for various applications.
34773587	3	56	dep	Halomonas	467:475	arg1	elongata					477:484	elongata	477:484	elongata	477:484	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	1	57	theme	waste	150:154	arg1	biomass					156:162	waste biomass	150:162	waste biomass	150:162	Large quantities of waste biomass are generated annually worldwide by many industries and are vastly underutilized.
34773587	8	58	theme	suitable	1522:1529	arg1	properties					1511:1520	distinct physicochemical properties	1486:1520	distinct physicochemical properties suitable for various applications	1486:1554	Henceforth, the ability of Halomonas strains to use cost-effective substrates, especially cheese whey, is a promising approach for the production of EPS with distinct physicochemical properties suitable for various applications.
34773587	0	59	theme	exopolysaccharide	70:86	arg1	production					88:97	exopolysaccharide production	70:97	exopolysaccharide production by selected Halomonas strains	70:127	Sustainable use of agro-industrial wastes as potential feedstocks for exopolysaccharide production by selected Halomonas strains.
34773587	7	60	theme	lower	1228:1232	arg1	contents					1234:1241	lower contents	1228:1241	lower contents of galacturonic acid, ribose, and arabinose	1228:1285	The highest EPS concentration (446 mg/L) was obtained for H. caseinilytica K1 grown on cheese whey that produced an EPS composed mostly of galactose, rhamnose, glucose, and mannose, with lower contents of galacturonic acid, ribose, and arabinose and with a molecular weight of 54.5 kDa.
34773587	1	61	theme	biomass	156:162	arg1	quantities					136:145	Large quantities	130:145	Large quantities of waste biomass	130:162	Large quantities of waste biomass are generated annually worldwide by many industries and are vastly underutilized.
34773587	6	62	theme	EPS	992:994	arg1	weight					966:971	the average molecular weight	944:971	the average molecular weight of the synthesized EPS	944:994	Similarly, the average molecular weight of the synthesized EPS was affected, ranging from 54.5 to 4480 kDa.
34773587	3	63	theme	low-cost	614:621	arg1	source					665:670	the sole carbon source	649:670	the sole carbon source	649:670	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	3	63	theme	low-cost	614:621	arg1	wastes					639:644	low-cost agro-industrial wastes	614:644	low-cost agro-industrial wastes	614:644	In this study, four selected Halomonas strains, namely, Halomonas caseinilytica K1, Halomonas elongata K4, Halomonas smyrnensis S3, and Halomonas halophila S4, were investigated for the production of exopolysaccharides (EPS) using low-cost agro-industrial wastes as the sole carbon source: cheese whey, grape pomace, and glycerol.
34773587	5	64	theme	predominant	865:875	arg1	monomers					877:884	the predominant monomers	861:884	the predominant monomers	861:884	Glucose, mannose, galactose, and rhamnose were the predominant monomers, but their relative molar ratio was different.
34773587	5	64	theme	predominant	865:875	arg1	galactose					832:840	galactose	832:840	galactose	832:840	Glucose, mannose, galactose, and rhamnose were the predominant monomers, but their relative molar ratio was different.
34773587	5	64	theme	predominant	865:875	arg1	rhamnose					847:854	rhamnose	847:854	rhamnose	847:854	Glucose, mannose, galactose, and rhamnose were the predominant monomers, but their relative molar ratio was different.
34773587	5	64	theme	predominant	865:875	arg1	mannose					823:829	mannose	823:829	mannose	823:829	Glucose, mannose, galactose, and rhamnose were the predominant monomers, but their relative molar ratio was different.
34773587	5	64	theme	predominant	865:875	arg1	Glucose					814:820	Glucose	814:820	Glucose	814:820	Glucose, mannose, galactose, and rhamnose were the predominant monomers, but their relative molar ratio was different.
34773587	6	65	theme	synthesized	980:990	arg1	EPS					992:994	the synthesized EPS	976:994	the synthesized EPS	976:994	Similarly, the average molecular weight of the synthesized EPS was affected, ranging from 54.5 to 4480 kDa.
32299048	0	0	theme	mixed	91:95	arg1	cultures					107:114	mixed microbial cultures	91:114	mixed microbial cultures	91:114	Strategy for biological co-production of levulinic acid and polyhydroxyalkanoates by using mixed microbial cultures fed with synthetic hemicellulose hydrolysate.
32299048	1	1	theme	microbial	253:261	arg1	cultures					263:270	mixed microbial cultures	247:270	mixed microbial cultures (MMC)	247:276	Hemicellulose hydrolysates (HH), which could be an interesting carbon source to feed mixed microbial cultures (MMC) able to accumulate high value-added compounds.
32299048	1	1	theme	microbial	253:261	arg1	MMC					273:275	MMC	273:275	MMC	273:275	Hemicellulose hydrolysates (HH), which could be an interesting carbon source to feed mixed microbial cultures (MMC) able to accumulate high value-added compounds.
32299048	4	2	theme	%	801:801	arg1	pentoses					803:810	85% pentoses	799:810	85% pentoses	799:810	This work proved that the cultivation strategy used allowed the biological production of LA, reaching 37%w/w when the SHH was composed of 85% pentoses.
32299048	5	3	from	addition	816:823	arg1	production					854:863	the simultaneous biological production	826:863	the simultaneous biological production of LA and PHB	826:877	In addition, the simultaneous biological production of LA and PHB was possible when the SHH was enriched with acetate (45% pentoses - 50% acetate).
32299048	5	3	from	addition	816:823	arg1	possible					883:890	possible	883:890	possible	883:890	In addition, the simultaneous biological production of LA and PHB was possible when the SHH was enriched with acetate (45% pentoses - 50% acetate).
32299048	5	4	theme	PHB	875:877	arg1	production					854:863	the simultaneous biological production	826:863	the simultaneous biological production of LA and PHB	826:877	In addition, the simultaneous biological production of LA and PHB was possible when the SHH was enriched with acetate (45% pentoses - 50% acetate).
32299048	5	4	theme	PHB	875:877	arg1	possible					883:890	possible	883:890	possible	883:890	In addition, the simultaneous biological production of LA and PHB was possible when the SHH was enriched with acetate (45% pentoses - 50% acetate).
32299048	0	5	theme	microbial	97:105	arg1	cultures					107:114	mixed microbial cultures	91:114	mixed microbial cultures	91:114	Strategy for biological co-production of levulinic acid and polyhydroxyalkanoates by using mixed microbial cultures fed with synthetic hemicellulose hydrolysate.
32299048	6	6	theme	SHH	1016:1018	arg1	composition					997:1007	the composition	993:1007	the composition of the SHH	993:1018	Finally, this study showed that the composition of the SHH impacts directly on the selected microorganism genus and the type and quantity of the value-added compounds obtained.
32299048	4	7	theme	85	799:800	arg1	%					801:801	%	801:801	%	801:801	This work proved that the cultivation strategy used allowed the biological production of LA, reaching 37%w/w when the SHH was composed of 85% pentoses.
32299048	4	8	theme	biological	725:734	arg1	production					736:745	the biological production	721:745	the biological production of LA	721:751	This work proved that the cultivation strategy used allowed the biological production of LA, reaching 37%w/w when the SHH was composed of 85% pentoses.
32299048	2	9	theme	Polyhydroxyalcanoates	464:484	arg1	production					426:435	the simultaneous biological production	398:435	the simultaneous biological production of Levulinic Acid (LA) and Polyhydroxyalcanoates (PHA) by MMC fed with a synthetic HH (SHH)	398:527	This research focused on the evaluation of a culture strategy to achieve the simultaneous biological production of Levulinic Acid (LA) and Polyhydroxyalcanoates (PHA) by MMC fed with a synthetic HH (SHH).
32299048	6	10	theme	value-added	1106:1116	arg1	compounds					1118:1126	the value-added compounds	1102:1126	the value-added compounds obtained	1102:1135	Finally, this study showed that the composition of the SHH impacts directly on the selected microorganism genus and the type and quantity of the value-added compounds obtained.
32299048	2	11	theme	strategy	378:385	arg1	evaluation					354:363	the evaluation	350:363	the evaluation of a culture strategy	350:385	This research focused on the evaluation of a culture strategy to achieve the simultaneous biological production of Levulinic Acid (LA) and Polyhydroxyalcanoates (PHA) by MMC fed with a synthetic HH (SHH).
32299048	2	12	theme	Acid	450:453	arg1	production					426:435	the simultaneous biological production	398:435	the simultaneous biological production of Levulinic Acid (LA) and Polyhydroxyalcanoates (PHA) by MMC fed with a synthetic HH (SHH)	398:527	This research focused on the evaluation of a culture strategy to achieve the simultaneous biological production of Levulinic Acid (LA) and Polyhydroxyalcanoates (PHA) by MMC fed with a synthetic HH (SHH).
32299048	3	13	theme	culture	534:540	arg1	strategy					542:549	The culture strategy	530:549	The culture strategy	530:549	The culture strategy involves the use of sequential batch reactors (SBR) to select microorganisms capable of producing LA and PHA.
32299048	2	14	theme	culture	370:376	arg1	strategy					378:385	a culture strategy	368:385	a culture strategy	368:385	This research focused on the evaluation of a culture strategy to achieve the simultaneous biological production of Levulinic Acid (LA) and Polyhydroxyalcanoates (PHA) by MMC fed with a synthetic HH (SHH).
32299048	2	15	theme	Levulinic	440:448	arg1	Acid					450:453	Levulinic Acid	440:453	Levulinic Acid (LA)	440:458	This research focused on the evaluation of a culture strategy to achieve the simultaneous biological production of Levulinic Acid (LA) and Polyhydroxyalcanoates (PHA) by MMC fed with a synthetic HH (SHH).
32299048	2	15	theme	Levulinic	440:448	arg1	LA					456:457	LA	456:457	LA	456:457	This research focused on the evaluation of a culture strategy to achieve the simultaneous biological production of Levulinic Acid (LA) and Polyhydroxyalcanoates (PHA) by MMC fed with a synthetic HH (SHH).
32299048	0	16	theme	hemicellulose	135:147	arg1	hydrolysate					149:159	synthetic hemicellulose hydrolysate	125:159	synthetic hemicellulose hydrolysate	125:159	Strategy for biological co-production of levulinic acid and polyhydroxyalkanoates by using mixed microbial cultures fed with synthetic hemicellulose hydrolysate.
32299048	5	17	theme	LA	868:869	arg1	production					854:863	the simultaneous biological production	826:863	the simultaneous biological production of LA and PHB	826:877	In addition, the simultaneous biological production of LA and PHB was possible when the SHH was enriched with acetate (45% pentoses - 50% acetate).
32299048	5	17	theme	LA	868:869	arg1	possible					883:890	possible	883:890	possible	883:890	In addition, the simultaneous biological production of LA and PHB was possible when the SHH was enriched with acetate (45% pentoses - 50% acetate).
32299048	0	18	theme	biological	13:22	arg1	co-production					24:36	biological co-production	13:36	biological co-production of levulinic acid and polyhydroxyalkanoates by using mixed microbial cultures	13:114	Strategy for biological co-production of levulinic acid and polyhydroxyalkanoates by using mixed microbial cultures fed with synthetic hemicellulose hydrolysate.
32299048	6	19	theme	selected	1044:1051	arg1	genus					1067:1071	the selected microorganism genus	1040:1071	the selected microorganism genus	1040:1071	Finally, this study showed that the composition of the SHH impacts directly on the selected microorganism genus and the type and quantity of the value-added compounds obtained.
32299048	0	20	theme	synthetic	125:133	arg1	hydrolysate					149:159	synthetic hemicellulose hydrolysate	125:159	synthetic hemicellulose hydrolysate	125:159	Strategy for biological co-production of levulinic acid and polyhydroxyalkanoates by using mixed microbial cultures fed with synthetic hemicellulose hydrolysate.
32299048	5	21	theme	%	949:949	arg1	acetate					951:957	50% acetate	947:957	50% acetate	947:957	In addition, the simultaneous biological production of LA and PHB was possible when the SHH was enriched with acetate (45% pentoses - 50% acetate).
32299048	4	22	theme	cultivation	687:697	arg1	strategy					699:706	the cultivation strategy	683:706	the cultivation strategy used	683:711	This work proved that the cultivation strategy used allowed the biological production of LA, reaching 37%w/w when the SHH was composed of 85% pentoses.
32299048	2	23	theme	biological	415:424	arg1	production					426:435	the simultaneous biological production	398:435	the simultaneous biological production of Levulinic Acid (LA) and Polyhydroxyalcanoates (PHA) by MMC fed with a synthetic HH (SHH)	398:527	This research focused on the evaluation of a culture strategy to achieve the simultaneous biological production of Levulinic Acid (LA) and Polyhydroxyalcanoates (PHA) by MMC fed with a synthetic HH (SHH).
32299048	0	24	theme	levulinic	41:49	arg1	acid					51:54	levulinic acid	41:54	levulinic acid	41:54	Strategy for biological co-production of levulinic acid and polyhydroxyalkanoates by using mixed microbial cultures fed with synthetic hemicellulose hydrolysate.
32299048	1	25	theme	interesting	213:223	arg1	hydrolysates					176:187	Hemicellulose hydrolysates	162:187	Hemicellulose hydrolysates (HH)	162:192	Hemicellulose hydrolysates (HH), which could be an interesting carbon source to feed mixed microbial cultures (MMC) able to accumulate high value-added compounds.
32299048	1	25	theme	interesting	213:223	arg1	source					232:237	an interesting carbon source	210:237	an interesting carbon source	210:237	Hemicellulose hydrolysates (HH), which could be an interesting carbon source to feed mixed microbial cultures (MMC) able to accumulate high value-added compounds.
32299048	5	26	theme	%	934:934	arg1	pentoses					936:943	45% pentoses	932:943	45% pentoses	932:943	In addition, the simultaneous biological production of LA and PHB was possible when the SHH was enriched with acetate (45% pentoses - 50% acetate).
32299048	5	27	dep	acetate	923:929	arg1	acetate					951:957	50% acetate	947:957	50% acetate	947:957	In addition, the simultaneous biological production of LA and PHB was possible when the SHH was enriched with acetate (45% pentoses - 50% acetate).
32299048	5	27	dep	acetate	923:929	arg1	pentoses					936:943	45% pentoses	932:943	45% pentoses	932:943	In addition, the simultaneous biological production of LA and PHB was possible when the SHH was enriched with acetate (45% pentoses - 50% acetate).
32299048	2	28	theme	simultaneous	402:413	arg1	production					426:435	the simultaneous biological production	398:435	the simultaneous biological production of Levulinic Acid (LA) and Polyhydroxyalcanoates (PHA) by MMC fed with a synthetic HH (SHH)	398:527	This research focused on the evaluation of a culture strategy to achieve the simultaneous biological production of Levulinic Acid (LA) and Polyhydroxyalcanoates (PHA) by MMC fed with a synthetic HH (SHH).
32299048	6	29	theme	compounds	1118:1126	arg1	quantity					1090:1097	quantity	1090:1097	quantity	1090:1097	Finally, this study showed that the composition of the SHH impacts directly on the selected microorganism genus and the type and quantity of the value-added compounds obtained.
32299048	6	29	theme	compounds	1118:1126	arg1	genus					1067:1071	the selected microorganism genus	1040:1071	the selected microorganism genus	1040:1071	Finally, this study showed that the composition of the SHH impacts directly on the selected microorganism genus and the type and quantity of the value-added compounds obtained.
32299048	6	29	theme	compounds	1118:1126	arg1	type					1081:1084	type	1081:1084	type	1081:1084	Finally, this study showed that the composition of the SHH impacts directly on the selected microorganism genus and the type and quantity of the value-added compounds obtained.
32299048	1	30	theme	high	297:300	arg1	compounds					314:322	high value-added compounds	297:322	high value-added compounds	297:322	Hemicellulose hydrolysates (HH), which could be an interesting carbon source to feed mixed microbial cultures (MMC) able to accumulate high value-added compounds.
32299048	2	31	theme	synthetic	510:518	arg1	SHH					524:526	SHH	524:526	SHH	524:526	This research focused on the evaluation of a culture strategy to achieve the simultaneous biological production of Levulinic Acid (LA) and Polyhydroxyalcanoates (PHA) by MMC fed with a synthetic HH (SHH).
32299048	2	31	theme	synthetic	510:518	arg1	HH					520:521	a synthetic HH	508:521	a synthetic HH (SHH)	508:527	This research focused on the evaluation of a culture strategy to achieve the simultaneous biological production of Levulinic Acid (LA) and Polyhydroxyalcanoates (PHA) by MMC fed with a synthetic HH (SHH).
32299048	1	32	theme	carbon	225:230	arg1	hydrolysates					176:187	Hemicellulose hydrolysates	162:187	Hemicellulose hydrolysates (HH)	162:192	Hemicellulose hydrolysates (HH), which could be an interesting carbon source to feed mixed microbial cultures (MMC) able to accumulate high value-added compounds.
32299048	1	32	theme	carbon	225:230	arg1	source					232:237	an interesting carbon source	210:237	an interesting carbon source	210:237	Hemicellulose hydrolysates (HH), which could be an interesting carbon source to feed mixed microbial cultures (MMC) able to accumulate high value-added compounds.
32299048	1	33	theme	value-added	302:312	arg1	compounds					314:322	high value-added compounds	297:322	high value-added compounds	297:322	Hemicellulose hydrolysates (HH), which could be an interesting carbon source to feed mixed microbial cultures (MMC) able to accumulate high value-added compounds.
32299048	4	34	theme	w/w	766:768	arg1	%					765:765	37%w/w	763:768	37%w/w when the SHH was composed of 85% pentoses	763:810	This work proved that the cultivation strategy used allowed the biological production of LA, reaching 37%w/w when the SHH was composed of 85% pentoses.
32299048	0	35	theme	acid	51:54	arg1	co-production					24:36	biological co-production	13:36	biological co-production of levulinic acid and polyhydroxyalkanoates by using mixed microbial cultures	13:114	Strategy for biological co-production of levulinic acid and polyhydroxyalkanoates by using mixed microbial cultures fed with synthetic hemicellulose hydrolysate.
32299048	3	36	theme	capable	628:634	arg1	microorganisms					613:626	microorganisms	613:626	microorganisms capable of producing LA and PHA	613:658	The culture strategy involves the use of sequential batch reactors (SBR) to select microorganisms capable of producing LA and PHA.
32299048	5	37	theme	45	932:933	arg1	%					934:934	%	934:934	%	934:934	In addition, the simultaneous biological production of LA and PHB was possible when the SHH was enriched with acetate (45% pentoses - 50% acetate).
32299048	4	38	theme	LA	750:751	arg1	production					736:745	the biological production	721:745	the biological production of LA	721:751	This work proved that the cultivation strategy used allowed the biological production of LA, reaching 37%w/w when the SHH was composed of 85% pentoses.
32299048	3	39	theme	batch	582:586	arg1	SBR					598:600	SBR	598:600	SBR	598:600	The culture strategy involves the use of sequential batch reactors (SBR) to select microorganisms capable of producing LA and PHA.
32299048	3	39	theme	batch	582:586	arg1	reactors					588:595	sequential batch reactors	571:595	sequential batch reactors (SBR)	571:601	The culture strategy involves the use of sequential batch reactors (SBR) to select microorganisms capable of producing LA and PHA.
32299048	5	40	theme	simultaneous	830:841	arg1	production					854:863	the simultaneous biological production	826:863	the simultaneous biological production of LA and PHB	826:877	In addition, the simultaneous biological production of LA and PHB was possible when the SHH was enriched with acetate (45% pentoses - 50% acetate).
32299048	5	40	theme	simultaneous	830:841	arg1	possible					883:890	possible	883:890	possible	883:890	In addition, the simultaneous biological production of LA and PHB was possible when the SHH was enriched with acetate (45% pentoses - 50% acetate).
32299048	3	41	theme	reactors	588:595	arg1	use					564:566	the use	560:566	the use of sequential batch reactors (SBR) to select microorganisms capable of producing LA and PHA	560:658	The culture strategy involves the use of sequential batch reactors (SBR) to select microorganisms capable of producing LA and PHA.
32299048	5	42	theme	biological	843:852	arg1	production					854:863	the simultaneous biological production	826:863	the simultaneous biological production of LA and PHB	826:877	In addition, the simultaneous biological production of LA and PHB was possible when the SHH was enriched with acetate (45% pentoses - 50% acetate).
32299048	5	42	theme	biological	843:852	arg1	possible					883:890	possible	883:890	possible	883:890	In addition, the simultaneous biological production of LA and PHB was possible when the SHH was enriched with acetate (45% pentoses - 50% acetate).
32299048	6	43	theme	microorganism	1053:1065	arg1	genus					1067:1071	the selected microorganism genus	1040:1071	the selected microorganism genus	1040:1071	Finally, this study showed that the composition of the SHH impacts directly on the selected microorganism genus and the type and quantity of the value-added compounds obtained.
32299048	5	44	theme	50	947:948	arg1	%					949:949	%	949:949	%	949:949	In addition, the simultaneous biological production of LA and PHB was possible when the SHH was enriched with acetate (45% pentoses - 50% acetate).
32299048	1	45	theme	Hemicellulose	162:174	arg1	hydrolysates					176:187	Hemicellulose hydrolysates	162:187	Hemicellulose hydrolysates (HH)	162:192	Hemicellulose hydrolysates (HH), which could be an interesting carbon source to feed mixed microbial cultures (MMC) able to accumulate high value-added compounds.
32299048	1	45	theme	Hemicellulose	162:174	arg1	source					232:237	an interesting carbon source	210:237	an interesting carbon source	210:237	Hemicellulose hydrolysates (HH), which could be an interesting carbon source to feed mixed microbial cultures (MMC) able to accumulate high value-added compounds.
32299048	1	45	theme	Hemicellulose	162:174	arg1	HH					190:191	HH	190:191	HH	190:191	Hemicellulose hydrolysates (HH), which could be an interesting carbon source to feed mixed microbial cultures (MMC) able to accumulate high value-added compounds.
32299048	0	46	theme	polyhydroxyalkanoates	60:80	arg1	co-production					24:36	biological co-production	13:36	biological co-production of levulinic acid and polyhydroxyalkanoates by using mixed microbial cultures	13:114	Strategy for biological co-production of levulinic acid and polyhydroxyalkanoates by using mixed microbial cultures fed with synthetic hemicellulose hydrolysate.
32299048	3	47	theme	sequential	571:580	arg1	SBR					598:600	SBR	598:600	SBR	598:600	The culture strategy involves the use of sequential batch reactors (SBR) to select microorganisms capable of producing LA and PHA.
32299048	3	47	theme	sequential	571:580	arg1	reactors					588:595	sequential batch reactors	571:595	sequential batch reactors (SBR)	571:601	The culture strategy involves the use of sequential batch reactors (SBR) to select microorganisms capable of producing LA and PHA.
32299048	5	48	from	possible	883:890	arg1	addition					816:823	addition	816:823	addition	816:823	In addition, the simultaneous biological production of LA and PHB was possible when the SHH was enriched with acetate (45% pentoses - 50% acetate).
32299048	1	49	theme	mixed	247:251	arg1	cultures					263:270	mixed microbial cultures	247:270	mixed microbial cultures (MMC)	247:276	Hemicellulose hydrolysates (HH), which could be an interesting carbon source to feed mixed microbial cultures (MMC) able to accumulate high value-added compounds.
32299048	1	49	theme	mixed	247:251	arg1	MMC					273:275	MMC	273:275	MMC	273:275	Hemicellulose hydrolysates (HH), which could be an interesting carbon source to feed mixed microbial cultures (MMC) able to accumulate high value-added compounds.
33421465	3	0	theme	composition	331:341	arg1	analysis					343:350	Monosaccharide composition analysis	316:350	Monosaccharide composition analysis	316:350	Monosaccharide composition analysis indicated that LCP-05 was composed of Man, Rha, GlcA, GalA, Glc, Gal and Ara in a molar ratio of 0.83:1.68:0.33:2.15:1.00:1.45:1.22.
33421465	5	1	theme	Araf	748:751	arg1	residues					762:769	the Araf and Glcp residues	744:769	residues	762:769	The side chains are terminated primarily with the Araf and Glcp residues.
33421465	4	2	theme	Rha	684:686	arg1	residues					688:695	the Rha residues	680:695	the Rha residues	680:695	The framework of LCP-05 was speculated to be a branched rhamnogalacturonan with the backbone consisting of α-1,2,4-linked Rhap and α-1,4-linked GalAp, and bearing branches at the O-4 position of the Rha residues.
33421465	3	3	from	Man	390:392	arg1	ratio					440:444	a molar ratio	432:444	a molar ratio of 0.83:1.68:0.33:2.15:1.00:1.45:1.22	432:482	Monosaccharide composition analysis indicated that LCP-05 was composed of Man, Rha, GlcA, GalA, Glc, Gal and Ara in a molar ratio of 0.83:1.68:0.33:2.15:1.00:1.45:1.22.
33421465	6	4	theme	RAW	865:867	arg1	cells					875:879	RAW 264.7 cells	865:879	RAW 264.7 cells	865:879	LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
33421465	4	5	theme	LCP-05	502:507	arg1	framework					489:497	The framework	485:497	The framework of LCP-05	485:507	The framework of LCP-05 was speculated to be a branched rhamnogalacturonan with the backbone consisting of α-1,2,4-linked Rhap and α-1,4-linked GalAp, and bearing branches at the O-4 position of the Rha residues.
33421465	4	6	link	α-1,4-linked	616:627	arg1	GalAp					629:633	α-1,4-linked GalAp	616:633	α-1,4-linked GalAp	616:633	The framework of LCP-05 was speculated to be a branched rhamnogalacturonan with the backbone consisting of α-1,2,4-linked Rhap and α-1,4-linked GalAp, and bearing branches at the O-4 position of the Rha residues.
33421465	0	7	from	Isolation	0:8	arg1	flowers					47:53	the flowers	43:53	the flowers of Leucosceptrum canum Smith	43:82	Isolation of an acidic polysaccharide from the flowers of Leucosceptrum canum Smith and its immunomodulatory activity evaluation.
33421465	6	8	theme	suppressive	913:923	arg1	effect					925:930	cell's suppressive effect	906:930	RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells	896:1005	LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
33421465	0	9	from	flowers	47:53	arg1	Isolation					0:8	Isolation	0:8	Isolation of an acidic polysaccharide from the flowers of Leucosceptrum canum Smith	0:82	Isolation of an acidic polysaccharide from the flowers of Leucosceptrum canum Smith and its immunomodulatory activity evaluation.
33421465	0	9	from	flowers	47:53	arg1	polysaccharide					23:36	an acidic polysaccharide	13:36	an acidic polysaccharide from the flowers of Leucosceptrum canum Smith	13:82	Isolation of an acidic polysaccharide from the flowers of Leucosceptrum canum Smith and its immunomodulatory activity evaluation.
33421465	0	9	from	flowers	47:53	arg1	activity					109:116	its immunomodulatory activity	88:116	its immunomodulatory activity	88:116	Isolation of an acidic polysaccharide from the flowers of Leucosceptrum canum Smith and its immunomodulatory activity evaluation.
33421465	4	10	with	rhamnogalacturonan	541:558	arg1	backbone					569:576	the backbone	565:576	the backbone consisting of α-1,2,4-linked Rhap and α-1,4-linked GalAp	565:633	The framework of LCP-05 was speculated to be a branched rhamnogalacturonan with the backbone consisting of α-1,2,4-linked Rhap and α-1,4-linked GalAp, and bearing branches at the O-4 position of the Rha residues.
33421465	2	11	theme	molecular	273:281	arg1	weight					283:288	a molecular weight	271:288	a molecular weight of approximately 8.9 kDa	271:313	LCP-05 was an acidic polysaccharide with a molecular weight of approximately 8.9 kDa.
33421465	6	12	theme	breast	987:992	arg1	cells					1001:1005	4 T1 mammary breast cancer cells	974:1005	4 T1 mammary breast cancer cells	974:1005	LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
33421465	6	13	theme	cells	1001:1005	arg1	growth					945:950	cell growth	940:950	cell growth	940:950	LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
33421465	6	13	theme	cells	1001:1005	arg1	migration					961:969	cell migration	956:969	cell migration	956:969	LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
33421465	0	14	theme	immunomodulatory	92:107	arg1	activity					109:116	its immunomodulatory activity	88:116	its immunomodulatory activity	88:116	Isolation of an acidic polysaccharide from the flowers of Leucosceptrum canum Smith and its immunomodulatory activity evaluation.
33421465	6	15	theme	IL-6	846:849	arg1	production					828:837	the production	824:837	the production of NO, IL-6, and TNF-α	824:860	LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
33421465	6	16	theme	cancer	994:999	arg1	cells					1001:1005	4 T1 mammary breast cancer cells	974:1005	4 T1 mammary breast cancer cells	974:1005	LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
33421465	4	17	theme	O-4	664:666	arg1	position					668:675	the O-4 position	660:675	the O-4 position of the Rha residues	660:695	The framework of LCP-05 was speculated to be a branched rhamnogalacturonan with the backbone consisting of α-1,2,4-linked Rhap and α-1,4-linked GalAp, and bearing branches at the O-4 position of the Rha residues.
33421465	4	18	theme	branched	532:539	arg1	rhamnogalacturonan					541:558	a branched rhamnogalacturonan	530:558	a branched rhamnogalacturonan with the backbone consisting of α-1,2,4-linked Rhap and α-1,4-linked GalAp	530:633	The framework of LCP-05 was speculated to be a branched rhamnogalacturonan with the backbone consisting of α-1,2,4-linked Rhap and α-1,4-linked GalAp, and bearing branches at the O-4 position of the Rha residues.
33421465	6	19	from	effect	925:930	arg1	growth					945:950	cell growth	940:950	cell growth	940:950	LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
33421465	6	19	from	effect	925:930	arg1	migration					961:969	cell migration	956:969	cell migration	956:969	LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
33421465	6	20	dep	RAW	896:898	arg1	effect					925:930	cell's suppressive effect	906:930	RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells	896:1005	LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
33421465	6	21	theme	mammary	979:985	arg1	cells					1001:1005	4 T1 mammary breast cancer cells	974:1005	4 T1 mammary breast cancer cells	974:1005	LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
33421465	0	22	theme	acidic	16:21	arg1	polysaccharide					23:36	an acidic polysaccharide	13:36	an acidic polysaccharide from the flowers of Leucosceptrum canum Smith	13:82	Isolation of an acidic polysaccharide from the flowers of Leucosceptrum canum Smith and its immunomodulatory activity evaluation.
33421465	6	23	dep	able	795:798	arg1	induce					817:822	induce	817:822	to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells	800:879	LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
33421465	6	23	dep	able	795:798	arg1	induce					889:894	induce	889:894	to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells	886:1005	LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
33421465	2	24	theme	8.9 kDa	307:313	arg1	weight					283:288	a molecular weight	271:288	a molecular weight of approximately 8.9 kDa	271:313	LCP-05 was an acidic polysaccharide with a molecular weight of approximately 8.9 kDa.
33421465	6	25	theme	4 T1	974:977	arg1	cells					1001:1005	4 T1 mammary breast cancer cells	974:1005	4 T1 mammary breast cancer cells	974:1005	LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
33421465	1	26	attach	isolated	174:181	arg2	LCP-05					162:167	LCP-05	162:167	LCP-05	162:167	A water-soluble polysaccharide (LCP-05) was isolated from the flowers of Leucosceptrum canum Smith.
33421465	1	26	attach	isolated	174:181	arg2	polysaccharide					146:159	A water-soluble polysaccharide	130:159	A water-soluble polysaccharide (LCP-05)	130:168	A water-soluble polysaccharide (LCP-05) was isolated from the flowers of Leucosceptrum canum Smith.
33421465	1	26	attach	isolated	174:181	arg1	flowers					192:198	the flowers	188:198	the flowers of Leucosceptrum canum Smith	188:227	A water-soluble polysaccharide (LCP-05) was isolated from the flowers of Leucosceptrum canum Smith.
33421465	3	27	theme	molar	434:438	arg1	ratio					440:444	a molar ratio	432:444	a molar ratio of 0.83:1.68:0.33:2.15:1.00:1.45:1.22	432:482	Monosaccharide composition analysis indicated that LCP-05 was composed of Man, Rha, GlcA, GalA, Glc, Gal and Ara in a molar ratio of 0.83:1.68:0.33:2.15:1.00:1.45:1.22.
33421465	5	28	theme	Glcp	757:760	arg1	residues					762:769	the Araf and Glcp residues	744:769	residues	762:769	The side chains are terminated primarily with the Araf and Glcp residues.
33421465	0	29	theme	polysaccharide	23:36	arg1	Isolation					0:8	Isolation	0:8	Isolation of an acidic polysaccharide from the flowers of Leucosceptrum canum Smith	0:82	Isolation of an acidic polysaccharide from the flowers of Leucosceptrum canum Smith and its immunomodulatory activity evaluation.
33421465	0	29	theme	polysaccharide	23:36	arg1	activity					109:116	its immunomodulatory activity	88:116	its immunomodulatory activity	88:116	Isolation of an acidic polysaccharide from the flowers of Leucosceptrum canum Smith and its immunomodulatory activity evaluation.
33421465	5	30	theme	side	702:705	arg1	chains					707:712	The side chains	698:712	The side chains	698:712	The side chains are terminated primarily with the Araf and Glcp residues.
33421465	6	31	theme	cell	940:943	arg1	growth					945:950	cell growth	940:950	cell growth	940:950	LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
33421465	6	32	theme	cell	956:959	arg1	migration					961:969	cell migration	956:969	cell migration	956:969	LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
33421465	4	33	link	α-1,2,4-linked	592:605	arg1	Rhap					607:610	α-1,2,4-linked Rhap	592:610	α-1,2,4-linked Rhap	592:610	The framework of LCP-05 was speculated to be a branched rhamnogalacturonan with the backbone consisting of α-1,2,4-linked Rhap and α-1,4-linked GalAp, and bearing branches at the O-4 position of the Rha residues.
33421465	6	34	theme	NO	842:843	arg1	production					828:837	the production	824:837	the production of NO, IL-6, and TNF-α	824:860	LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
33421465	3	35	theme	0.83:1.68:0.33:2.15:1.00:1.45:1.22	449:482	arg1	ratio					440:444	a molar ratio	432:444	a molar ratio of 0.83:1.68:0.33:2.15:1.00:1.45:1.22	432:482	Monosaccharide composition analysis indicated that LCP-05 was composed of Man, Rha, GlcA, GalA, Glc, Gal and Ara in a molar ratio of 0.83:1.68:0.33:2.15:1.00:1.45:1.22.
33421465	6	36	theme	TNF-α	856:860	arg1	production					828:837	the production	824:837	the production of NO, IL-6, and TNF-α	824:860	LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
33421465	3	37	theme	Monosaccharide	316:329	arg1	analysis					343:350	Monosaccharide composition analysis	316:350	Monosaccharide composition analysis	316:350	Monosaccharide composition analysis indicated that LCP-05 was composed of Man, Rha, GlcA, GalA, Glc, Gal and Ara in a molar ratio of 0.83:1.68:0.33:2.15:1.00:1.45:1.22.
33421465	0	38	from	activity	109:116	arg1	flowers					47:53	the flowers	43:53	the flowers of Leucosceptrum canum Smith	43:82	Isolation of an acidic polysaccharide from the flowers of Leucosceptrum canum Smith and its immunomodulatory activity evaluation.
33421465	4	39	theme	residues	688:695	arg1	position					668:675	the O-4 position	660:675	the O-4 position of the Rha residues	660:695	The framework of LCP-05 was speculated to be a branched rhamnogalacturonan with the backbone consisting of α-1,2,4-linked Rhap and α-1,4-linked GalAp, and bearing branches at the O-4 position of the Rha residues.
33421465	2	40	with	polysaccharide	251:264	arg1	weight					283:288	a molecular weight	271:288	a molecular weight of approximately 8.9 kDa	271:313	LCP-05 was an acidic polysaccharide with a molecular weight of approximately 8.9 kDa.
33421465	0	41	dep	Leucosceptrum	58:70	arg1	canum					72:76	Leucosceptrum canum Smith	58:82	Leucosceptrum canum Smith	58:82	Isolation of an acidic polysaccharide from the flowers of Leucosceptrum canum Smith and its immunomodulatory activity evaluation.
33421465	0	41	dep	Leucosceptrum	58:70	arg1	Smith					78:82	Smith	78:82	Smith	78:82	Isolation of an acidic polysaccharide from the flowers of Leucosceptrum canum Smith and its immunomodulatory activity evaluation.
33421465	1	42	theme	Smith	223:227	arg1	flowers					192:198	the flowers	188:198	the flowers of Leucosceptrum canum Smith	188:227	A water-soluble polysaccharide (LCP-05) was isolated from the flowers of Leucosceptrum canum Smith.
33421465	1	43	theme	water-soluble	132:144	arg1	LCP-05					162:167	LCP-05	162:167	LCP-05	162:167	A water-soluble polysaccharide (LCP-05) was isolated from the flowers of Leucosceptrum canum Smith.
33421465	1	43	theme	water-soluble	132:144	arg1	polysaccharide					146:159	A water-soluble polysaccharide	130:159	A water-soluble polysaccharide (LCP-05)	130:168	A water-soluble polysaccharide (LCP-05) was isolated from the flowers of Leucosceptrum canum Smith.
33421465	4	44	theme	α-1,2,4-linked	592:605	arg1	Rhap					607:610	α-1,2,4-linked Rhap	592:610	α-1,2,4-linked Rhap	592:610	The framework of LCP-05 was speculated to be a branched rhamnogalacturonan with the backbone consisting of α-1,2,4-linked Rhap and α-1,4-linked GalAp, and bearing branches at the O-4 position of the Rha residues.
33421465	0	45	theme	Leucosceptrum	58:70	arg1	flowers					47:53	the flowers	43:53	the flowers of Leucosceptrum canum Smith	43:82	Isolation of an acidic polysaccharide from the flowers of Leucosceptrum canum Smith and its immunomodulatory activity evaluation.
33421465	2	46	theme	acidic	244:249	arg1	LCP-05					230:235	LCP-05	230:235	LCP-05	230:235	LCP-05 was an acidic polysaccharide with a molecular weight of approximately 8.9 kDa.
33421465	2	46	theme	acidic	244:249	arg1	polysaccharide					251:264	an acidic polysaccharide	241:264	an acidic polysaccharide with a molecular weight of approximately 8.9 kDa	241:313	LCP-05 was an acidic polysaccharide with a molecular weight of approximately 8.9 kDa.
33421465	4	47	theme	α-1,4-linked	616:627	arg1	GalAp					629:633	α-1,4-linked GalAp	616:633	α-1,4-linked GalAp	616:633	The framework of LCP-05 was speculated to be a branched rhamnogalacturonan with the backbone consisting of α-1,2,4-linked Rhap and α-1,4-linked GalAp, and bearing branches at the O-4 position of the Rha residues.
33421465	0	48	dep	Isolation	0:8	arg1	evaluation					118:127	evaluation	118:127	evaluation	118:127	Isolation of an acidic polysaccharide from the flowers of Leucosceptrum canum Smith and its immunomodulatory activity evaluation.
32657144	2	0	theme	monosaccharides	687:701	arg1	composition					703:713	monosaccharides composition	687:713	monosaccharides composition	687:713	T. sinensis seeds polysaccharides (TSP) were separated and purified using DEAE-52 cellulose and Sephacryl S-300 column chromatography to obtain TSP-3a characterized of molecular weight, monosaccharides composition, and other preliminary structural features.
32657144	0	1	theme	sinensis	74:81	arg1	polysaccharides					49:63	polysaccharides	49:63	polysaccharides of Toona sinensis	49:81	Recovery of immune activity by administration of polysaccharides of Toona sinensis and its characterization of major component.
32657144	5	2	theme	1H	1041:1042	arg1	NMR					1044:1046	1H NMR	1041:1046	1H NMR	1041:1046	Combining 1H NMR, 13C NMR, and comparison with those reported in the literature, the backbone of TSP-3a might contain →6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-(1→.
32657144	5	3	theme	TSP-3a	1128:1133	arg1	backbone					1116:1123	the backbone	1112:1123	the backbone of TSP-3a	1112:1133	Combining 1H NMR, 13C NMR, and comparison with those reported in the literature, the backbone of TSP-3a might contain →6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-(1→.
32657144	1	4	theme	immunomodulatory	352:367	arg1	activity					369:376	immunomodulatory activity	352:376	immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo	352:498	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	5	theme	relative	418:425	arg1	indices					427:433	the relative indices	414:433	the relative indices of thymus and spleen	414:454	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	6	theme	macroporous	258:268	arg1	resin					274:278	macroporous 900 resin	258:278	macroporous 900 resin	258:278	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	2	7	theme	S-300	607:611	arg1	chromatography					620:633	DEAE-52 cellulose and Sephacryl S-300 column chromatography	575:633	chromatography	620:633	T. sinensis seeds polysaccharides (TSP) were separated and purified using DEAE-52 cellulose and Sephacryl S-300 column chromatography to obtain TSP-3a characterized of molecular weight, monosaccharides composition, and other preliminary structural features.
32657144	5	8	theme	-α-D-Gal-	1187:1195	arg1	1→					1210:1211	1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6	1162:1219	1→	1210:1211	Combining 1H NMR, 13C NMR, and comparison with those reported in the literature, the backbone of TSP-3a might contain →6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-(1→.
32657144	5	9	theme	1→	1197:1198	arg1	1→					1210:1211	1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6	1162:1219	1→	1210:1211	Combining 1H NMR, 13C NMR, and comparison with those reported in the literature, the backbone of TSP-3a might contain →6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-(1→.
32657144	0	10	theme	major	111:115	arg1	component					117:125	major component	111:125	major component	111:125	Recovery of immune activity by administration of polysaccharides of Toona sinensis and its characterization of major component.
32657144	5	11	contain	contain	1141:1147	arg2	-β-D-Gal-					1221:1229	→6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-	1149:1229	→6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-	1149:1229	Combining 1H NMR, 13C NMR, and comparison with those reported in the literature, the backbone of TSP-3a might contain →6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-(1→.
32657144	5	11	contain	contain	1141:1147	arg1	backbone					1116:1123	the backbone	1112:1123	the backbone of TSP-3a	1112:1133	Combining 1H NMR, 13C NMR, and comparison with those reported in the literature, the backbone of TSP-3a might contain →6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-(1→.
32657144	2	12	theme	Sephacryl	597:605	arg1	chromatography					620:633	DEAE-52 cellulose and Sephacryl S-300 column chromatography	575:633	chromatography	620:633	T. sinensis seeds polysaccharides (TSP) were separated and purified using DEAE-52 cellulose and Sephacryl S-300 column chromatography to obtain TSP-3a characterized of molecular weight, monosaccharides composition, and other preliminary structural features.
32657144	2	13	theme	structural	738:747	arg1	features					749:756	other preliminary structural features	720:756	other preliminary structural features	720:756	T. sinensis seeds polysaccharides (TSP) were separated and purified using DEAE-52 cellulose and Sephacryl S-300 column chromatography to obtain TSP-3a characterized of molecular weight, monosaccharides composition, and other preliminary structural features.
32657144	4	14	theme	FT-IR	902:906	arg1	analysis					908:915	FT-IR analysis	902:915	FT-IR analysis	902:915	FT-IR analysis suggested TSP-3a was an acid polysaccharide and the presence of β configuration within the TSP-3a was discovered.
32657144	5	15	theme	13C	1049:1051	arg1	NMR					1053:1055	13C NMR	1049:1055	13C NMR	1049:1055	Combining 1H NMR, 13C NMR, and comparison with those reported in the literature, the backbone of TSP-3a might contain →6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-(1→.
32657144	1	16	theme	thymus	438:443	arg1	indices					427:433	the relative indices	414:433	the relative indices of thymus and spleen	414:454	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	16	theme	thymus	438:443	arg1	index					478:482	the phagocytosis index	461:482	the phagocytosis index of mice in vivo	461:498	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	16	theme	thymus	438:443	arg1	levels					385:390	the levels	381:390	the levels of IgA, IgG, and IgM	381:411	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	2	17	theme	preliminary	726:736	arg1	features					749:756	other preliminary structural features	720:756	other preliminary structural features	720:756	T. sinensis seeds polysaccharides (TSP) were separated and purified using DEAE-52 cellulose and Sephacryl S-300 column chromatography to obtain TSP-3a characterized of molecular weight, monosaccharides composition, and other preliminary structural features.
32657144	2	18	theme	DEAE-52	575:581	arg1	cellulose					583:591	DEAE-52 cellulose and Sephacryl S-300 column chromatography	575:633	cellulose	583:591	T. sinensis seeds polysaccharides (TSP) were separated and purified using DEAE-52 cellulose and Sephacryl S-300 column chromatography to obtain TSP-3a characterized of molecular weight, monosaccharides composition, and other preliminary structural features.
32657144	1	19	with	deproteinization	285:300	arg1	resin					274:278	macroporous 900 resin	258:278	macroporous 900 resin	258:278	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	19	with	deproteinization	285:300	arg1	reagent					313:319	sevag reagent	307:319	sevag reagent	307:319	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	5	20	dep	-α-D-Glc-	1152:1160	arg1	→3					1166:1167	1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6	1162:1219	→3	1166:1167	Combining 1H NMR, 13C NMR, and comparison with those reported in the literature, the backbone of TSP-3a might contain →6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-(1→.
32657144	5	20	dep	-α-D-Glc-	1152:1160	arg1	→6					1184:1185	1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6	1162:1219	→6	1184:1185	Combining 1H NMR, 13C NMR, and comparison with those reported in the literature, the backbone of TSP-3a might contain →6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-(1→.
32657144	5	20	dep	-α-D-Glc-	1152:1160	arg1	→6					1218:1219	1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6	1162:1219	→6	1218:1219	Combining 1H NMR, 13C NMR, and comparison with those reported in the literature, the backbone of TSP-3a might contain →6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-(1→.
32657144	5	20	dep	-α-D-Glc-	1152:1160	arg1	α-D-Glc-					1201:1208	1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6	1162:1219	α-D-Glc-	1201:1208	Combining 1H NMR, 13C NMR, and comparison with those reported in the literature, the backbone of TSP-3a might contain →6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-(1→.
32657144	5	20	dep	-α-D-Glc-	1152:1160	arg1	1→					1210:1211	1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6	1162:1219	1→	1210:1211	Combining 1H NMR, 13C NMR, and comparison with those reported in the literature, the backbone of TSP-3a might contain →6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-(1→.
32657144	2	21	theme	other	720:724	arg1	features					749:756	other preliminary structural features	720:756	other preliminary structural features	720:756	T. sinensis seeds polysaccharides (TSP) were separated and purified using DEAE-52 cellulose and Sephacryl S-300 column chromatography to obtain TSP-3a characterized of molecular weight, monosaccharides composition, and other preliminary structural features.
32657144	1	22	theme	spleen	449:454	arg1	indices					427:433	the relative indices	414:433	the relative indices of thymus and spleen	414:454	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	22	theme	spleen	449:454	arg1	index					478:482	the phagocytosis index	461:482	the phagocytosis index of mice in vivo	461:498	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	22	theme	spleen	449:454	arg1	levels					385:390	the levels	381:390	the levels of IgA, IgG, and IgM	381:411	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	3	23	from	Rha	812:814	arg1	ratio					851:855	a molar ratio	843:855	a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21	843:899	TSP-3a (758.6 kDa) mainly contained Man, GalN, GlcA, Rha, Glc, Gal, Ara, and Fuc in a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21.
32657144	1	24	dep	in	492:493	arg1	vivo					495:498	vivo	495:498	vivo	495:498	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	25	theme	sinensis	183:190	arg1	seeds					171:175	the seeds	167:175	the seeds of T. sinensis	167:190	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	0	26	theme	activity	19:26	arg1	Recovery					0:7	Recovery	0:7	Recovery of immune activity by administration of polysaccharides of Toona sinensis and its characterization of major component.	0:126	Recovery of immune activity by administration of polysaccharides of Toona sinensis and its characterization of major component.
32657144	2	27	theme	seeds	513:517	arg1	TSP					536:538	TSP	536:538	TSP	536:538	T. sinensis seeds polysaccharides (TSP) were separated and purified using DEAE-52 cellulose and Sephacryl S-300 column chromatography to obtain TSP-3a characterized of molecular weight, monosaccharides composition, and other preliminary structural features.
32657144	2	27	theme	seeds	513:517	arg1	polysaccharides					519:533	seeds polysaccharides	513:533	T. sinensis seeds polysaccharides (TSP)	501:539	T. sinensis seeds polysaccharides (TSP) were separated and purified using DEAE-52 cellulose and Sephacryl S-300 column chromatography to obtain TSP-3a characterized of molecular weight, monosaccharides composition, and other preliminary structural features.
32657144	3	28	theme	0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21	860:899	arg1	ratio					851:855	a molar ratio	843:855	a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21	843:899	TSP-3a (758.6 kDa) mainly contained Man, GalN, GlcA, Rha, Glc, Gal, Ara, and Fuc in a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21.
32657144	2	29	theme	molecular	669:677	arg1	weight					679:684	molecular weight	669:684	molecular weight	669:684	T. sinensis seeds polysaccharides (TSP) were separated and purified using DEAE-52 cellulose and Sephacryl S-300 column chromatography to obtain TSP-3a characterized of molecular weight, monosaccharides composition, and other preliminary structural features.
32657144	0	30	theme	immune	12:17	arg1	activity					19:26	immune activity	12:26	immune activity	12:26	Recovery of immune activity by administration of polysaccharides of Toona sinensis and its characterization of major component.
32657144	0	31	theme	component	117:125	arg1	administration					31:44	administration	31:44	administration of polysaccharides of Toona sinensis	31:81	Recovery of immune activity by administration of polysaccharides of Toona sinensis and its characterization of major component.
32657144	0	31	theme	component	117:125	arg1	characterization					91:106	its characterization	87:106	its characterization of major component	87:125	Recovery of immune activity by administration of polysaccharides of Toona sinensis and its characterization of major component.
32657144	3	32	from	GalN	800:803	arg1	ratio					851:855	a molar ratio	843:855	a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21	843:899	TSP-3a (758.6 kDa) mainly contained Man, GalN, GlcA, Rha, Glc, Gal, Ara, and Fuc in a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21.
32657144	3	33	from	Glc	817:819	arg1	ratio					851:855	a molar ratio	843:855	a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21	843:899	TSP-3a (758.6 kDa) mainly contained Man, GalN, GlcA, Rha, Glc, Gal, Ara, and Fuc in a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21.
32657144	4	34	theme	configuration	983:995	arg1	presence					969:976	the presence	965:976	the presence of β configuration within the TSP-3a	965:1013	FT-IR analysis suggested TSP-3a was an acid polysaccharide and the presence of β configuration within the TSP-3a was discovered.
32657144	1	35	theme	hot	195:197	arg1	extraction					205:214	hot water extraction	195:214	hot water extraction	195:214	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	2	36	theme	T.	501:502	arg1	TSP					536:538	TSP	536:538	TSP	536:538	T. sinensis seeds polysaccharides (TSP) were separated and purified using DEAE-52 cellulose and Sephacryl S-300 column chromatography to obtain TSP-3a characterized of molecular weight, monosaccharides composition, and other preliminary structural features.
32657144	2	36	theme	T.	501:502	arg1	polysaccharides					519:533	seeds polysaccharides	513:533	T. sinensis seeds polysaccharides (TSP)	501:539	T. sinensis seeds polysaccharides (TSP) were separated and purified using DEAE-52 cellulose and Sephacryl S-300 column chromatography to obtain TSP-3a characterized of molecular weight, monosaccharides composition, and other preliminary structural features.
32657144	1	37	theme	IgA	395:397	arg1	indices					427:433	the relative indices	414:433	the relative indices of thymus and spleen	414:454	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	37	theme	IgA	395:397	arg1	index					478:482	the phagocytosis index	461:482	the phagocytosis index of mice in vivo	461:498	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	37	theme	IgA	395:397	arg1	levels					385:390	the levels	381:390	the levels of IgA, IgG, and IgM	381:411	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	38	with	extraction	205:214	arg1	resin					274:278	macroporous 900 resin	258:278	macroporous 900 resin	258:278	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	38	with	extraction	205:214	arg1	reagent					313:319	sevag reagent	307:319	sevag reagent	307:319	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	3	39	theme	molar	845:849	arg1	ratio					851:855	a molar ratio	843:855	a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21	843:899	TSP-3a (758.6 kDa) mainly contained Man, GalN, GlcA, Rha, Glc, Gal, Ara, and Fuc in a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21.
32657144	1	40	theme	water	199:203	arg1	extraction					205:214	hot water extraction	195:214	hot water extraction	195:214	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	4	41	theme	acid	941:944	arg1	polysaccharide					946:959	an acid polysaccharide	938:959	an acid polysaccharide	938:959	FT-IR analysis suggested TSP-3a was an acid polysaccharide and the presence of β configuration within the TSP-3a was discovered.
32657144	3	42	from	GlcA	806:809	arg1	ratio					851:855	a molar ratio	843:855	a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21	843:899	TSP-3a (758.6 kDa) mainly contained Man, GalN, GlcA, Rha, Glc, Gal, Ara, and Fuc in a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21.
32657144	1	43	from	activity	369:376	arg1	indices					427:433	the relative indices	414:433	the relative indices of thymus and spleen	414:454	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	43	from	activity	369:376	arg1	index					478:482	the phagocytosis index	461:482	the phagocytosis index of mice in vivo	461:498	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	43	from	activity	369:376	arg1	levels					385:390	the levels	381:390	the levels of IgA, IgG, and IgM	381:411	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	0	44	theme	polysaccharides	49:63	arg1	administration					31:44	administration	31:44	administration of polysaccharides of Toona sinensis	31:81	Recovery of immune activity by administration of polysaccharides of Toona sinensis and its characterization of major component.
32657144	0	44	theme	polysaccharides	49:63	arg1	characterization					91:106	its characterization	87:106	its characterization of major component	87:125	Recovery of immune activity by administration of polysaccharides of Toona sinensis and its characterization of major component.
32657144	1	45	theme	sevag	307:311	arg1	reagent					313:319	sevag reagent	307:319	sevag reagent	307:319	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	46	theme	IgG	400:402	arg1	indices					427:433	the relative indices	414:433	the relative indices of thymus and spleen	414:454	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	46	theme	IgG	400:402	arg1	index					478:482	the phagocytosis index	461:482	the phagocytosis index of mice in vivo	461:498	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	46	theme	IgG	400:402	arg1	levels					385:390	the levels	381:390	the levels of IgA, IgG, and IgM	381:411	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	6	47	dep	[Figure	1234:1240	arg1	see					1243:1245	see	1243:1245	see text	1243:1250	[Figure: see text].
32657144	5	48	dep	contain	1141:1147	arg1	1→					1231:1232	1→	1231:1232	1→	1231:1232	Combining 1H NMR, 13C NMR, and comparison with those reported in the literature, the backbone of TSP-3a might contain →6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-(1→.
32657144	1	49	theme	phagocytosis	465:476	arg1	index					478:482	the phagocytosis index	461:482	the phagocytosis index of mice in vivo	461:498	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	5	50	theme	-β-D-GlcA-	1169:1178	arg1	1→					1210:1211	1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6	1162:1219	1→	1210:1211	Combining 1H NMR, 13C NMR, and comparison with those reported in the literature, the backbone of TSP-3a might contain →6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-(1→.
32657144	4	51	theme	β	981:981	arg1	configuration					983:995	β configuration	981:995	β configuration	981:995	FT-IR analysis suggested TSP-3a was an acid polysaccharide and the presence of β configuration within the TSP-3a was discovered.
32657144	2	52	theme	column	613:618	arg1	chromatography					620:633	DEAE-52 cellulose and Sephacryl S-300 column chromatography	575:633	chromatography	620:633	T. sinensis seeds polysaccharides (TSP) were separated and purified using DEAE-52 cellulose and Sephacryl S-300 column chromatography to obtain TSP-3a characterized of molecular weight, monosaccharides composition, and other preliminary structural features.
32657144	1	53	contain	possessed	328:336	arg1	reagent					313:319	sevag reagent	307:319	sevag reagent	307:319	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	53	contain	possessed	328:336	arg2	activity					369:376	immunomodulatory activity	352:376	immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo	352:498	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	3	54	from	Man	795:797	arg1	ratio					851:855	a molar ratio	843:855	a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21	843:899	TSP-3a (758.6 kDa) mainly contained Man, GalN, GlcA, Rha, Glc, Gal, Ara, and Fuc in a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21.
32657144	1	55	theme	IgM	409:411	arg1	indices					427:433	the relative indices	414:433	the relative indices of thymus and spleen	414:454	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	55	theme	IgM	409:411	arg1	index					478:482	the phagocytosis index	461:482	the phagocytosis index of mice in vivo	461:498	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	55	theme	IgM	409:411	arg1	levels					385:390	the levels	381:390	the levels of IgA, IgG, and IgM	381:411	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	56	with	precipitation	225:237	arg1	resin					274:278	macroporous 900 resin	258:278	macroporous 900 resin	258:278	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	56	with	precipitation	225:237	arg1	reagent					313:319	sevag reagent	307:319	sevag reagent	307:319	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	5	57	theme	1→	1180:1181	arg1	1→					1210:1211	1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6	1162:1219	1→	1210:1211	Combining 1H NMR, 13C NMR, and comparison with those reported in the literature, the backbone of TSP-3a might contain →6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-(1→.
32657144	1	58	theme	ethanol	217:223	arg1	precipitation					225:237	ethanol precipitation	217:237	ethanol precipitation	217:237	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	0	59	theme	Toona	68:72	arg1	sinensis					74:81	Toona sinensis	68:81	Toona sinensis	68:81	Recovery of immune activity by administration of polysaccharides of Toona sinensis and its characterization of major component.
32657144	4	60	dep	suggested	917:925	arg1	polysaccharide					946:959	an acid polysaccharide	938:959	an acid polysaccharide	938:959	FT-IR analysis suggested TSP-3a was an acid polysaccharide and the presence of β configuration within the TSP-3a was discovered.
32657144	3	61	from	Fuc	836:838	arg1	ratio					851:855	a molar ratio	843:855	a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21	843:899	TSP-3a (758.6 kDa) mainly contained Man, GalN, GlcA, Rha, Glc, Gal, Ara, and Fuc in a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21.
32657144	1	62	theme	mice	487:490	arg1	indices					427:433	the relative indices	414:433	the relative indices of thymus and spleen	414:454	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	62	theme	mice	487:490	arg1	index					478:482	the phagocytosis index	461:482	the phagocytosis index of mice in vivo	461:498	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	62	theme	mice	487:490	arg1	levels					385:390	the levels	381:390	the levels of IgA, IgG, and IgM	381:411	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	5	63	theme	-α-D-Glc-	1152:1160	arg1	-β-D-Gal-					1221:1229	→6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-	1149:1229	→6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-	1149:1229	Combining 1H NMR, 13C NMR, and comparison with those reported in the literature, the backbone of TSP-3a might contain →6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-(1→.
32657144	2	64	dep	polysaccharides	519:533	arg1	sinensis					504:511	T. sinensis seeds polysaccharides (TSP)	501:539	T. sinensis seeds polysaccharides (TSP)	501:539	T. sinensis seeds polysaccharides (TSP) were separated and purified using DEAE-52 cellulose and Sephacryl S-300 column chromatography to obtain TSP-3a characterized of molecular weight, monosaccharides composition, and other preliminary structural features.
32657144	3	65	contain	contained	785:793	arg2	GlcA					806:809	GlcA	806:809	GlcA	806:809	TSP-3a (758.6 kDa) mainly contained Man, GalN, GlcA, Rha, Glc, Gal, Ara, and Fuc in a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21.
32657144	3	65	contain	contained	785:793	arg2	Fuc					836:838	Fuc	836:838	Fuc	836:838	TSP-3a (758.6 kDa) mainly contained Man, GalN, GlcA, Rha, Glc, Gal, Ara, and Fuc in a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21.
32657144	3	65	contain	contained	785:793	arg2	Ara					827:829	Ara	827:829	Ara	827:829	TSP-3a (758.6 kDa) mainly contained Man, GalN, GlcA, Rha, Glc, Gal, Ara, and Fuc in a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21.
32657144	3	65	contain	contained	785:793	arg1	TSP-3a					759:764	TSP-3a	759:764	TSP-3a (758.6 kDa)	759:776	TSP-3a (758.6 kDa) mainly contained Man, GalN, GlcA, Rha, Glc, Gal, Ara, and Fuc in a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21.
32657144	3	65	contain	contained	785:793	arg1	758.6 kDa					767:775	758.6 kDa	767:775	758.6 kDa	767:775	TSP-3a (758.6 kDa) mainly contained Man, GalN, GlcA, Rha, Glc, Gal, Ara, and Fuc in a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21.
32657144	3	65	contain	contained	785:793	arg2	Man					795:797	Man	795:797	Man	795:797	TSP-3a (758.6 kDa) mainly contained Man, GalN, GlcA, Rha, Glc, Gal, Ara, and Fuc in a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21.
32657144	3	65	contain	contained	785:793	arg2	Rha					812:814	Rha	812:814	Rha	812:814	TSP-3a (758.6 kDa) mainly contained Man, GalN, GlcA, Rha, Glc, Gal, Ara, and Fuc in a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21.
32657144	3	65	contain	contained	785:793	arg2	GalN					800:803	GalN	800:803	GalN	800:803	TSP-3a (758.6 kDa) mainly contained Man, GalN, GlcA, Rha, Glc, Gal, Ara, and Fuc in a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21.
32657144	3	65	contain	contained	785:793	arg2	Glc					817:819	Glc	817:819	Glc	817:819	TSP-3a (758.6 kDa) mainly contained Man, GalN, GlcA, Rha, Glc, Gal, Ara, and Fuc in a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21.
32657144	1	66	theme	in	492:493	arg1	mice					487:490	mice	487:490	mice in vivo	487:498	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	67	with	decoloration	240:251	arg1	resin					274:278	macroporous 900 resin	258:278	macroporous 900 resin	258:278	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	1	67	with	decoloration	240:251	arg1	reagent					313:319	sevag reagent	307:319	sevag reagent	307:319	The polysaccharides were acquired from the seeds of T. sinensis by hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice in vivo.
32657144	5	68	theme	1→	1162:1163	arg1	1→					1210:1211	1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6	1162:1219	1→	1210:1211	Combining 1H NMR, 13C NMR, and comparison with those reported in the literature, the backbone of TSP-3a might contain →6)-α-D-Glc-(1→, →3)-β-D-GlcA-(1→, →6)-α-D-Gal-(1→, α-D-Glc-(1→, and →6)-β-D-Gal-(1→.
32214327	10	0	theme	monomeric	1264:1272	arg1	35kD					1282:1285	35kD	1282:1285	35kD	1282:1285	TRACP 5a is a less active monomeric enzyme (35kD), with the intact loop peptide and TRACP 5b is proteolytically cleaved highly active enzyme encompassing two subunits (23 kD and 16 kD) held together by disulfide bonds.
32214327	10	0	theme	monomeric	1264:1272	arg1	5a					1244:1245	TRACP 5a	1238:1245	TRACP 5a	1238:1245	TRACP 5a is a less active monomeric enzyme (35kD), with the intact loop peptide and TRACP 5b is proteolytically cleaved highly active enzyme encompassing two subunits (23 kD and 16 kD) held together by disulfide bonds.
32214327	10	0	theme	monomeric	1264:1272	arg1	enzyme					1274:1279	a less active monomeric enzyme	1250:1279	a less active monomeric enzyme (35kD)	1250:1286	TRACP 5a is a less active monomeric enzyme (35kD), with the intact loop peptide and TRACP 5b is proteolytically cleaved highly active enzyme encompassing two subunits (23 kD and 16 kD) held together by disulfide bonds.
32214327	5	1	theme	wild	736:739	arg1	type					746:749	wild (WT) type	736:749	wild (WT) type	736:749	Analysis of wild (WT) type and eight mutant stable cell lines indicated that all mutants lacked stainable enzyme activity.
32214327	5	1	theme	wild	736:739	arg1	WT					742:743	WT	742:743	WT	742:743	Analysis of wild (WT) type and eight mutant stable cell lines indicated that all mutants lacked stainable enzyme activity.
32214327	6	2	theme	mutant	856:861	arg1	constructs					863:872	All ACP5 mutant constructs	847:872	All ACP5 mutant constructs	847:872	All ACP5 mutant constructs were translated into intact proteins by HEK-293 cells.
32214327	15	3	theme	bone	2360:2363	arg1	metabolism					2365:2374	dysregulated bone metabolism	2347:2374	dysregulated bone metabolism	2347:2374	In conclusion, determining the structure-function relationship of the SPENCD mutations in TRACP will expand our understanding of basic mechanisms underlying immune responsiveness and its involvement in dysregulated bone metabolism.
32214327	11	4	theme	significant	1589:1599	arg1	amounts					1601:1607	significant amounts	1589:1607	significant amounts	1589:1607	None of the mutant proteins were proteolytically processed into isoform 5b intracellularly, and only three mutants were secreted in significant amounts into the culture medium as intact isoform 5a-like proteins.
32214327	15	5	theme	mutations	2222:2230	arg1	relationship					2195:2206	the structure-function relationship	2172:2206	the structure-function relationship of the SPENCD mutations in TRACP	2172:2239	In conclusion, determining the structure-function relationship of the SPENCD mutations in TRACP will expand our understanding of basic mechanisms underlying immune responsiveness and its involvement in dysregulated bone metabolism.
32214327	15	6	from	involvement	2332:2342	arg1	metabolism					2365:2374	dysregulated bone metabolism	2347:2374	dysregulated bone metabolism	2347:2374	In conclusion, determining the structure-function relationship of the SPENCD mutations in TRACP will expand our understanding of basic mechanisms underlying immune responsiveness and its involvement in dysregulated bone metabolism.
32214327	12	7	theme	native	1769:1774	arg1	conformation					1776:1787	some native conformation	1764:1787	some native conformation	1764:1787	Analysis of antibody reactivity patterns revealed that T89I and M264K mutant proteins retained some native conformation, whereas all others were in "denatured" or "unfolded" forms.
32214327	14	8	theme	mutant	2025:2030	arg1	proteins					2032:2039	All mutant proteins	2021:2039	All mutant proteins	2021:2039	All mutant proteins were attacked by Endo-H sensitive glycans and none could be activated by proteolytic cleavage in vitro.
32214327	13	9	theme	inactive	2003:2010	arg1	T89I					1977:1980	mutant T89I	1970:1980	mutant T89I	1970:1980	Western blot analysis with intracellular and secreted TRACP proteins also revealed similar observations indicating that mutant T89I is amply secreted as inactive protein.
32214327	13	9	theme	inactive	2003:2010	arg1	protein					2012:2018	inactive protein	2003:2018	inactive protein	2003:2018	Western blot analysis with intracellular and secreted TRACP proteins also revealed similar observations indicating that mutant T89I is amply secreted as inactive protein.
32214327	13	10	with	analysis	1863:1870	arg1	proteins					1910:1917	intracellular and secreted TRACP proteins	1877:1917	intracellular and secreted TRACP proteins	1877:1917	Western blot analysis with intracellular and secreted TRACP proteins also revealed similar observations indicating that mutant T89I is amply secreted as inactive protein.
32214327	4	11	theme	antibodies	712:721	arg1	panel					692:696	a panel	690:696	a panel of monoclonal antibodies	690:721	TRACP expression was assessed by cytochemical and immuno-cytochemical staining with a panel of monoclonal antibodies.
32214327	5	12	theme	stable	768:773	arg1	lines					780:784	eight mutant stable cell lines	755:784	eight mutant stable cell lines	755:784	Analysis of wild (WT) type and eight mutant stable cell lines indicated that all mutants lacked stainable enzyme activity.
32214327	7	13	theme	gross	1077:1081	arg1	inhibition					1083:1092	no gross inhibition	1074:1092	no gross inhibition of TRACP biosynthesis by the mutations	1074:1131	The mutant TRACP proteins displayed variable immune reactivity patterns, and all drastically reduced enzymatic activity, revealing that there is no gross inhibition of TRACP biosynthesis by the mutations.
32214327	15	14	theme	basic	2274:2278	arg1	mechanisms					2280:2289	basic mechanisms	2274:2289	basic mechanisms underlying immune responsiveness and its involvement in dysregulated bone metabolism	2274:2374	In conclusion, determining the structure-function relationship of the SPENCD mutations in TRACP will expand our understanding of basic mechanisms underlying immune responsiveness and its involvement in dysregulated bone metabolism.
32214327	15	15	from	relationship	2195:2206	arg1	TRACP					2235:2239	TRACP	2235:2239	TRACP	2235:2239	In conclusion, determining the structure-function relationship of the SPENCD mutations in TRACP will expand our understanding of basic mechanisms underlying immune responsiveness and its involvement in dysregulated bone metabolism.
32214327	11	16	theme	isoform	1521:1527	arg1	5b					1529:1530	isoform 5b	1521:1530	isoform 5b	1521:1530	None of the mutant proteins were proteolytically processed into isoform 5b intracellularly, and only three mutants were secreted in significant amounts into the culture medium as intact isoform 5a-like proteins.
32214327	7	17	theme	TRACP	1097:1101	arg1	biosynthesis					1103:1114	TRACP biosynthesis	1097:1114	TRACP biosynthesis	1097:1114	The mutant TRACP proteins displayed variable immune reactivity patterns, and all drastically reduced enzymatic activity, revealing that there is no gross inhibition of TRACP biosynthesis by the mutations.
32214327	1	18	theme	tartrate-resistant	171:188	arg1	phosphatase					195:205	tartrate-resistant acid phosphatase	171:205	tartrate-resistant acid phosphatase (TRACP)	171:213	Biallelic mutations in ACP5, encoding tartrate-resistant acid phosphatase (TRACP), have recently been identified to cause the inherited immuno-osseous disorder, spondyloenchondrodysplasia (SPENCD).
32214327	1	18	theme	tartrate-resistant	171:188	arg1	TRACP					208:212	TRACP	208:212	TRACP	208:212	Biallelic mutations in ACP5, encoding tartrate-resistant acid phosphatase (TRACP), have recently been identified to cause the inherited immuno-osseous disorder, spondyloenchondrodysplasia (SPENCD).
32214327	15	19	theme	dysregulated	2347:2358	arg1	metabolism					2365:2374	dysregulated bone metabolism	2347:2374	dysregulated bone metabolism	2347:2374	In conclusion, determining the structure-function relationship of the SPENCD mutations in TRACP will expand our understanding of basic mechanisms underlying immune responsiveness and its involvement in dysregulated bone metabolism.
32214327	5	20	theme	type	746:749	arg1	Analysis					724:731	Analysis	724:731	Analysis of wild (WT) type and eight mutant stable cell lines	724:784	Analysis of wild (WT) type and eight mutant stable cell lines indicated that all mutants lacked stainable enzyme activity.
32214327	10	21	theme	intact	1298:1303	arg1	peptide					1310:1316	the intact loop peptide	1294:1316	the intact loop peptide	1294:1316	TRACP 5a is a less active monomeric enzyme (35kD), with the intact loop peptide and TRACP 5b is proteolytically cleaved highly active enzyme encompassing two subunits (23 kD and 16 kD) held together by disulfide bonds.
32214327	5	22	theme	stainable	820:828	arg1	activity					837:844	stainable enzyme activity	820:844	stainable enzyme activity	820:844	Analysis of wild (WT) type and eight mutant stable cell lines indicated that all mutants lacked stainable enzyme activity.
32214327	7	23	theme	enzymatic	1030:1038	arg1	activity					1040:1047	all drastically reduced enzymatic activity	1006:1047	all drastically reduced enzymatic activity	1006:1047	The mutant TRACP proteins displayed variable immune reactivity patterns, and all drastically reduced enzymatic activity, revealing that there is no gross inhibition of TRACP biosynthesis by the mutations.
32214327	12	24	dep	T89I	1724:1727	arg1	proteins					1746:1753	mutant proteins	1739:1753	mutant proteins	1739:1753	Analysis of antibody reactivity patterns revealed that T89I and M264K mutant proteins retained some native conformation, whereas all others were in "denatured" or "unfolded" forms.
32214327	5	25	theme	lines	780:784	arg1	Analysis					724:731	Analysis	724:731	Analysis of wild (WT) type and eight mutant stable cell lines	724:784	Analysis of wild (WT) type and eight mutant stable cell lines indicated that all mutants lacked stainable enzyme activity.
32214327	12	26	theme	reactivity	1690:1699	arg1	patterns					1701:1708	antibody reactivity patterns	1681:1708	antibody reactivity patterns	1681:1708	Analysis of antibody reactivity patterns revealed that T89I and M264K mutant proteins retained some native conformation, whereas all others were in "denatured" or "unfolded" forms.
32214327	10	27	theme	TRACP	1322:1326	arg1	5b					1328:1329	TRACP 5b	1322:1329	TRACP 5b	1322:1329	TRACP 5a is a less active monomeric enzyme (35kD), with the intact loop peptide and TRACP 5b is proteolytically cleaved highly active enzyme encompassing two subunits (23 kD and 16 kD) held together by disulfide bonds.
32214327	2	28	theme	TRACP	445:449	arg1	expression					451:460	TRACP expression	445:460	TRACP expression	445:460	This study was undertaken to characterize the eight reported missense mutations in ACP5 associated with SPENCD on TRACP expression.
32214327	13	29	theme	secreted	1895:1902	arg1	proteins					1910:1917	intracellular and secreted TRACP proteins	1877:1917	intracellular and secreted TRACP proteins	1877:1917	Western blot analysis with intracellular and secreted TRACP proteins also revealed similar observations indicating that mutant T89I is amply secreted as inactive protein.
32214327	1	30	theme	Biallelic	133:141	arg1	mutations					143:151	Biallelic mutations	133:151	Biallelic mutations	133:151	Biallelic mutations in ACP5, encoding tartrate-resistant acid phosphatase (TRACP), have recently been identified to cause the inherited immuno-osseous disorder, spondyloenchondrodysplasia (SPENCD).
32214327	10	31	with	enzyme	1274:1279	arg1	peptide					1310:1316	the intact loop peptide	1294:1316	the intact loop peptide	1294:1316	TRACP 5a is a less active monomeric enzyme (35kD), with the intact loop peptide and TRACP 5b is proteolytically cleaved highly active enzyme encompassing two subunits (23 kD and 16 kD) held together by disulfide bonds.
32214327	13	32	theme	Western	1850:1856	arg1	analysis					1863:1870	Western blot analysis	1850:1870	Western blot analysis with intracellular and secreted TRACP proteins	1850:1917	Western blot analysis with intracellular and secreted TRACP proteins also revealed similar observations indicating that mutant T89I is amply secreted as inactive protein.
32214327	3	33	theme	human	516:520	arg1	HEK-293					540:546	HEK-293	540:546	HEK-293	540:546	ACP5 mutant genes were synthesized, transfected into human embryonic kidney (HEK-293) cells and stably expressing cell lines were established.
32214327	3	33	theme	human	516:520	arg1	kidney					532:537	human embryonic kidney	516:537	human embryonic kidney (HEK-293) cells	516:553	ACP5 mutant genes were synthesized, transfected into human embryonic kidney (HEK-293) cells and stably expressing cell lines were established.
32214327	0	34	theme	ACP5	20:23	arg1	mutations					34:42	ACP5 missense mutations	20:42	ACP5 missense mutations encoding tartrate-resistant acid phosphatase	20:87	Characterisation of ACP5 missense mutations encoding tartrate-resistant acid phosphatase associated with spondyloenchondrodysplasia.
32214327	3	35	theme	ACP5	463:466	arg1	genes					475:479	ACP5 mutant genes	463:479	ACP5 mutant genes	463:479	ACP5 mutant genes were synthesized, transfected into human embryonic kidney (HEK-293) cells and stably expressing cell lines were established.
32214327	10	36	theme	loop	1305:1308	arg1	peptide					1310:1316	the intact loop peptide	1294:1316	the intact loop peptide	1294:1316	TRACP 5a is a less active monomeric enzyme (35kD), with the intact loop peptide and TRACP 5b is proteolytically cleaved highly active enzyme encompassing two subunits (23 kD and 16 kD) held together by disulfide bonds.
32214327	11	37	theme	mutant	1469:1474	arg1	proteins					1476:1483	the mutant proteins	1465:1483	the mutant proteins	1465:1483	None of the mutant proteins were proteolytically processed into isoform 5b intracellularly, and only three mutants were secreted in significant amounts into the culture medium as intact isoform 5a-like proteins.
32214327	0	38	theme	mutations	34:42	arg1	Characterisation					0:15	Characterisation	0:15	Characterisation of ACP5 missense mutations encoding tartrate-resistant acid phosphatase	0:87	Characterisation of ACP5 missense mutations encoding tartrate-resistant acid phosphatase associated with spondyloenchondrodysplasia.
32214327	15	39	theme	immune	2302:2307	arg1	responsiveness					2309:2322	immune responsiveness	2302:2322	immune responsiveness	2302:2322	In conclusion, determining the structure-function relationship of the SPENCD mutations in TRACP will expand our understanding of basic mechanisms underlying immune responsiveness and its involvement in dysregulated bone metabolism.
32214327	6	40	theme	HEK-293	914:920	arg1	cells					922:926	HEK-293 cells	914:926	HEK-293 cells	914:926	All ACP5 mutant constructs were translated into intact proteins by HEK-293 cells.
32214327	12	41	theme	unfolded	1833:1840	arg1	forms					1843:1847	"unfolded" forms	1832:1847	"unfolded" forms	1832:1847	Analysis of antibody reactivity patterns revealed that T89I and M264K mutant proteins retained some native conformation, whereas all others were in "denatured" or "unfolded" forms.
32214327	2	42	theme	missense	392:399	arg1	mutations					401:409	the eight reported missense mutations	373:409	the eight reported missense mutations in ACP5 associated with SPENCD on TRACP expression	373:460	This study was undertaken to characterize the eight reported missense mutations in ACP5 associated with SPENCD on TRACP expression.
32214327	0	43	theme	tartrate-resistant	53:70	arg1	phosphatase					77:87	tartrate-resistant acid phosphatase	53:87	tartrate-resistant acid phosphatase	53:87	Characterisation of ACP5 missense mutations encoding tartrate-resistant acid phosphatase associated with spondyloenchondrodysplasia.
32214327	15	44	theme	mechanisms	2280:2289	arg1	understanding					2257:2269	our understanding	2253:2269	our understanding of basic mechanisms underlying immune responsiveness and its involvement in dysregulated bone metabolism	2253:2374	In conclusion, determining the structure-function relationship of the SPENCD mutations in TRACP will expand our understanding of basic mechanisms underlying immune responsiveness and its involvement in dysregulated bone metabolism.
32214327	10	45	theme	disulfide	1440:1448	arg1	bonds					1450:1454	disulfide bonds	1440:1454	disulfide bonds	1440:1454	TRACP 5a is a less active monomeric enzyme (35kD), with the intact loop peptide and TRACP 5b is proteolytically cleaved highly active enzyme encompassing two subunits (23 kD and 16 kD) held together by disulfide bonds.
32214327	10	46	theme	active	1365:1370	arg1	enzyme					1372:1377	highly active enzyme	1358:1377	highly active enzyme encompassing two subunits (23 kD and 16 kD) held together by disulfide bonds	1358:1454	TRACP 5a is a less active monomeric enzyme (35kD), with the intact loop peptide and TRACP 5b is proteolytically cleaved highly active enzyme encompassing two subunits (23 kD and 16 kD) held together by disulfide bonds.
32214327	3	47	theme	kidney	532:537	arg1	cells					549:553	human embryonic kidney (HEK-293) cells	516:553	human embryonic kidney (HEK-293) cells	516:553	ACP5 mutant genes were synthesized, transfected into human embryonic kidney (HEK-293) cells and stably expressing cell lines were established.
32214327	7	48	theme	reactivity	981:990	arg1	patterns					992:999	variable immune reactivity patterns	965:999	variable immune reactivity patterns	965:999	The mutant TRACP proteins displayed variable immune reactivity patterns, and all drastically reduced enzymatic activity, revealing that there is no gross inhibition of TRACP biosynthesis by the mutations.
32214327	2	49	from	SPENCD	435:440	arg1	expression					451:460	TRACP expression	445:460	TRACP expression	445:460	This study was undertaken to characterize the eight reported missense mutations in ACP5 associated with SPENCD on TRACP expression.
32214327	12	50	theme	denatured	1818:1826	arg1	"					1827:1827	"denatured"	1817:1827	"denatured"	1817:1827	Analysis of antibody reactivity patterns revealed that T89I and M264K mutant proteins retained some native conformation, whereas all others were in "denatured" or "unfolded" forms.
32214327	4	51	theme	immuno-cytochemical	656:674	arg1	staining					676:683	cytochemical and immuno-cytochemical staining	639:683	cytochemical and immuno-cytochemical staining with a panel of monoclonal antibodies	639:721	TRACP expression was assessed by cytochemical and immuno-cytochemical staining with a panel of monoclonal antibodies.
32214327	11	52	theme	5a-like	1651:1657	arg1	mutants					1564:1570	only three mutants	1553:1570	only three mutants	1553:1570	None of the mutant proteins were proteolytically processed into isoform 5b intracellularly, and only three mutants were secreted in significant amounts into the culture medium as intact isoform 5a-like proteins.
32214327	11	52	theme	5a-like	1651:1657	arg1	proteins					1659:1666	intact isoform 5a-like proteins	1636:1666	intact isoform 5a-like proteins	1636:1666	None of the mutant proteins were proteolytically processed into isoform 5b intracellularly, and only three mutants were secreted in significant amounts into the culture medium as intact isoform 5a-like proteins.
32214327	7	53	theme	variable	965:972	arg1	patterns					992:999	variable immune reactivity patterns	965:999	variable immune reactivity patterns	965:999	The mutant TRACP proteins displayed variable immune reactivity patterns, and all drastically reduced enzymatic activity, revealing that there is no gross inhibition of TRACP biosynthesis by the mutations.
32214327	1	54	theme	immuno-osseous	269:282	arg1	spondyloenchondrodysplasia					294:319	spondyloenchondrodysplasia	294:319	spondyloenchondrodysplasia (SPENCD)	294:328	Biallelic mutations in ACP5, encoding tartrate-resistant acid phosphatase (TRACP), have recently been identified to cause the inherited immuno-osseous disorder, spondyloenchondrodysplasia (SPENCD).
32214327	1	54	theme	immuno-osseous	269:282	arg1	disorder					284:291	the inherited immuno-osseous disorder	255:291	the inherited immuno-osseous disorder	255:291	Biallelic mutations in ACP5, encoding tartrate-resistant acid phosphatase (TRACP), have recently been identified to cause the inherited immuno-osseous disorder, spondyloenchondrodysplasia (SPENCD).
32214327	11	55	theme	intact	1636:1641	arg1	mutants					1564:1570	only three mutants	1553:1570	only three mutants	1553:1570	None of the mutant proteins were proteolytically processed into isoform 5b intracellularly, and only three mutants were secreted in significant amounts into the culture medium as intact isoform 5a-like proteins.
32214327	11	55	theme	intact	1636:1641	arg1	proteins					1659:1666	intact isoform 5a-like proteins	1636:1666	intact isoform 5a-like proteins	1636:1666	None of the mutant proteins were proteolytically processed into isoform 5b intracellularly, and only three mutants were secreted in significant amounts into the culture medium as intact isoform 5a-like proteins.
32214327	2	56	from	mutations	401:409	arg1	ACP5					414:417	ACP5	414:417	ACP5 associated with SPENCD on TRACP expression	414:460	This study was undertaken to characterize the eight reported missense mutations in ACP5 associated with SPENCD on TRACP expression.
32214327	7	57	theme	mutant	933:938	arg1	proteins					946:953	The mutant TRACP proteins	929:953	The mutant TRACP proteins	929:953	The mutant TRACP proteins displayed variable immune reactivity patterns, and all drastically reduced enzymatic activity, revealing that there is no gross inhibition of TRACP biosynthesis by the mutations.
32214327	10	58	theme	active	1257:1262	arg1	35kD					1282:1285	35kD	1282:1285	35kD	1282:1285	TRACP 5a is a less active monomeric enzyme (35kD), with the intact loop peptide and TRACP 5b is proteolytically cleaved highly active enzyme encompassing two subunits (23 kD and 16 kD) held together by disulfide bonds.
32214327	10	58	theme	active	1257:1262	arg1	5a					1244:1245	TRACP 5a	1238:1245	TRACP 5a	1238:1245	TRACP 5a is a less active monomeric enzyme (35kD), with the intact loop peptide and TRACP 5b is proteolytically cleaved highly active enzyme encompassing two subunits (23 kD and 16 kD) held together by disulfide bonds.
32214327	10	58	theme	active	1257:1262	arg1	enzyme					1274:1279	a less active monomeric enzyme	1250:1279	a less active monomeric enzyme (35kD)	1250:1286	TRACP 5a is a less active monomeric enzyme (35kD), with the intact loop peptide and TRACP 5b is proteolytically cleaved highly active enzyme encompassing two subunits (23 kD and 16 kD) held together by disulfide bonds.
32214327	15	59	from	responsiveness	2309:2322	arg1	metabolism					2365:2374	dysregulated bone metabolism	2347:2374	dysregulated bone metabolism	2347:2374	In conclusion, determining the structure-function relationship of the SPENCD mutations in TRACP will expand our understanding of basic mechanisms underlying immune responsiveness and its involvement in dysregulated bone metabolism.
32214327	4	60	theme	cytochemical	639:650	arg1	staining					676:683	cytochemical and immuno-cytochemical staining	639:683	cytochemical and immuno-cytochemical staining with a panel of monoclonal antibodies	639:721	TRACP expression was assessed by cytochemical and immuno-cytochemical staining with a panel of monoclonal antibodies.
32214327	6	61	theme	ACP5	851:854	arg1	constructs					863:872	All ACP5 mutant constructs	847:872	All ACP5 mutant constructs	847:872	All ACP5 mutant constructs were translated into intact proteins by HEK-293 cells.
32214327	3	62	theme	cell	577:580	arg1	lines					582:586	stably expressing cell lines	559:586	stably expressing cell lines	559:586	ACP5 mutant genes were synthesized, transfected into human embryonic kidney (HEK-293) cells and stably expressing cell lines were established.
32214327	10	63	dep	subunits	1396:1403	arg1	kD					1419:1420	16 kD	1416:1420	16 kD	1416:1420	TRACP 5a is a less active monomeric enzyme (35kD), with the intact loop peptide and TRACP 5b is proteolytically cleaved highly active enzyme encompassing two subunits (23 kD and 16 kD) held together by disulfide bonds.
32214327	10	63	dep	subunits	1396:1403	arg1	subunits					1396:1403	two subunits	1392:1403	two subunits (23 kD and 16 kD) held together by disulfide bonds	1392:1454	TRACP 5a is a less active monomeric enzyme (35kD), with the intact loop peptide and TRACP 5b is proteolytically cleaved highly active enzyme encompassing two subunits (23 kD and 16 kD) held together by disulfide bonds.
32214327	10	63	dep	subunits	1396:1403	arg1	kD					1409:1410	23 kD	1406:1410	23 kD	1406:1410	TRACP 5a is a less active monomeric enzyme (35kD), with the intact loop peptide and TRACP 5b is proteolytically cleaved highly active enzyme encompassing two subunits (23 kD and 16 kD) held together by disulfide bonds.
32214327	15	64	theme	SPENCD	2215:2220	arg1	mutations					2222:2230	the SPENCD mutations	2211:2230	the SPENCD mutations in TRACP	2211:2239	In conclusion, determining the structure-function relationship of the SPENCD mutations in TRACP will expand our understanding of basic mechanisms underlying immune responsiveness and its involvement in dysregulated bone metabolism.
32214327	15	65	theme	structure-function	2176:2193	arg1	relationship					2195:2206	the structure-function relationship	2172:2206	the structure-function relationship of the SPENCD mutations in TRACP	2172:2239	In conclusion, determining the structure-function relationship of the SPENCD mutations in TRACP will expand our understanding of basic mechanisms underlying immune responsiveness and its involvement in dysregulated bone metabolism.
32214327	5	66	theme	mutant	761:766	arg1	lines					780:784	eight mutant stable cell lines	755:784	eight mutant stable cell lines	755:784	Analysis of wild (WT) type and eight mutant stable cell lines indicated that all mutants lacked stainable enzyme activity.
32214327	1	67	from	mutations	143:151	arg1	ACP5					156:159	ACP5	156:159	ACP5	156:159	Biallelic mutations in ACP5, encoding tartrate-resistant acid phosphatase (TRACP), have recently been identified to cause the inherited immuno-osseous disorder, spondyloenchondrodysplasia (SPENCD).
32214327	4	68	theme	monoclonal	701:710	arg1	antibodies					712:721	monoclonal antibodies	701:721	monoclonal antibodies	701:721	TRACP expression was assessed by cytochemical and immuno-cytochemical staining with a panel of monoclonal antibodies.
32214327	7	69	theme	biosynthesis	1103:1114	arg1	inhibition					1083:1092	no gross inhibition	1074:1092	no gross inhibition of TRACP biosynthesis by the mutations	1074:1131	The mutant TRACP proteins displayed variable immune reactivity patterns, and all drastically reduced enzymatic activity, revealing that there is no gross inhibition of TRACP biosynthesis by the mutations.
32214327	4	70	with	staining	676:683	arg1	panel					692:696	a panel	690:696	a panel of monoclonal antibodies	690:721	TRACP expression was assessed by cytochemical and immuno-cytochemical staining with a panel of monoclonal antibodies.
32214327	13	71	theme	similar	1933:1939	arg1	observations					1941:1952	similar observations	1933:1952	similar observations indicating that mutant T89I is amply secreted as inactive protein	1933:2018	Western blot analysis with intracellular and secreted TRACP proteins also revealed similar observations indicating that mutant T89I is amply secreted as inactive protein.
32214327	15	72	from	TRACP	2235:2239	arg1	relationship					2195:2206	the structure-function relationship	2172:2206	the structure-function relationship of the SPENCD mutations in TRACP	2172:2239	In conclusion, determining the structure-function relationship of the SPENCD mutations in TRACP will expand our understanding of basic mechanisms underlying immune responsiveness and its involvement in dysregulated bone metabolism.
32214327	12	73	theme	mutant	1739:1744	arg1	proteins					1746:1753	mutant proteins	1739:1753	mutant proteins	1739:1753	Analysis of antibody reactivity patterns revealed that T89I and M264K mutant proteins retained some native conformation, whereas all others were in "denatured" or "unfolded" forms.
32214327	1	74	theme	acid	190:193	arg1	phosphatase					195:205	tartrate-resistant acid phosphatase	171:205	tartrate-resistant acid phosphatase (TRACP)	171:213	Biallelic mutations in ACP5, encoding tartrate-resistant acid phosphatase (TRACP), have recently been identified to cause the inherited immuno-osseous disorder, spondyloenchondrodysplasia (SPENCD).
32214327	1	74	theme	acid	190:193	arg1	TRACP					208:212	TRACP	208:212	TRACP	208:212	Biallelic mutations in ACP5, encoding tartrate-resistant acid phosphatase (TRACP), have recently been identified to cause the inherited immuno-osseous disorder, spondyloenchondrodysplasia (SPENCD).
32214327	13	75	theme	mutant	1970:1975	arg1	T89I					1977:1980	mutant T89I	1970:1980	mutant T89I	1970:1980	Western blot analysis with intracellular and secreted TRACP proteins also revealed similar observations indicating that mutant T89I is amply secreted as inactive protein.
32214327	13	75	theme	mutant	1970:1975	arg1	protein					2012:2018	inactive protein	2003:2018	inactive protein	2003:2018	Western blot analysis with intracellular and secreted TRACP proteins also revealed similar observations indicating that mutant T89I is amply secreted as inactive protein.
32214327	13	76	theme	TRACP	1904:1908	arg1	proteins					1910:1917	intracellular and secreted TRACP proteins	1877:1917	intracellular and secreted TRACP proteins	1877:1917	Western blot analysis with intracellular and secreted TRACP proteins also revealed similar observations indicating that mutant T89I is amply secreted as inactive protein.
32214327	5	77	theme	cell	775:778	arg1	lines					780:784	eight mutant stable cell lines	755:784	eight mutant stable cell lines	755:784	Analysis of wild (WT) type and eight mutant stable cell lines indicated that all mutants lacked stainable enzyme activity.
32214327	14	78	theme	Endo-H	2058:2063	arg1	glycans					2075:2081	Endo-H sensitive glycans	2058:2081	Endo-H sensitive glycans	2058:2081	All mutant proteins were attacked by Endo-H sensitive glycans and none could be activated by proteolytic cleavage in vitro.
32214327	15	79	from	mutations	2222:2230	arg1	TRACP					2235:2239	TRACP	2235:2239	TRACP	2235:2239	In conclusion, determining the structure-function relationship of the SPENCD mutations in TRACP will expand our understanding of basic mechanisms underlying immune responsiveness and its involvement in dysregulated bone metabolism.
32214327	7	80	theme	reduced	1022:1028	arg1	activity					1040:1047	all drastically reduced enzymatic activity	1006:1047	all drastically reduced enzymatic activity	1006:1047	The mutant TRACP proteins displayed variable immune reactivity patterns, and all drastically reduced enzymatic activity, revealing that there is no gross inhibition of TRACP biosynthesis by the mutations.
32214327	12	81	theme	antibody	1681:1688	arg1	patterns					1701:1708	antibody reactivity patterns	1681:1708	antibody reactivity patterns	1681:1708	Analysis of antibody reactivity patterns revealed that T89I and M264K mutant proteins retained some native conformation, whereas all others were in "denatured" or "unfolded" forms.
32214327	12	82	theme	patterns	1701:1708	arg1	Analysis					1669:1676	Analysis	1669:1676	Analysis of antibody reactivity patterns	1669:1708	Analysis of antibody reactivity patterns revealed that T89I and M264K mutant proteins retained some native conformation, whereas all others were in "denatured" or "unfolded" forms.
32214327	4	83	theme	TRACP	606:610	arg1	expression					612:621	TRACP expression	606:621	TRACP expression	606:621	TRACP expression was assessed by cytochemical and immuno-cytochemical staining with a panel of monoclonal antibodies.
32214327	14	84	theme	proteolytic	2114:2124	arg1	cleavage					2126:2133	proteolytic cleavage	2114:2133	proteolytic cleavage	2114:2133	All mutant proteins were attacked by Endo-H sensitive glycans and none could be activated by proteolytic cleavage in vitro.
32214327	11	85	theme	proteins	1476:1483	arg1	None					1457:1460	None	1457:1460	None of the mutant proteins	1457:1483	None of the mutant proteins were proteolytically processed into isoform 5b intracellularly, and only three mutants were secreted in significant amounts into the culture medium as intact isoform 5a-like proteins.
32214327	0	86	theme	missense	25:32	arg1	mutations					34:42	ACP5 missense mutations	20:42	ACP5 missense mutations encoding tartrate-resistant acid phosphatase	20:87	Characterisation of ACP5 missense mutations encoding tartrate-resistant acid phosphatase associated with spondyloenchondrodysplasia.
32214327	13	87	theme	blot	1858:1861	arg1	analysis					1863:1870	Western blot analysis	1850:1870	Western blot analysis with intracellular and secreted TRACP proteins	1850:1917	Western blot analysis with intracellular and secreted TRACP proteins also revealed similar observations indicating that mutant T89I is amply secreted as inactive protein.
32214327	3	88	theme	embryonic	522:530	arg1	HEK-293					540:546	HEK-293	540:546	HEK-293	540:546	ACP5 mutant genes were synthesized, transfected into human embryonic kidney (HEK-293) cells and stably expressing cell lines were established.
32214327	3	88	theme	embryonic	522:530	arg1	kidney					532:537	human embryonic kidney	516:537	human embryonic kidney (HEK-293) cells	516:553	ACP5 mutant genes were synthesized, transfected into human embryonic kidney (HEK-293) cells and stably expressing cell lines were established.
32214327	5	89	theme	enzyme	830:835	arg1	activity					837:844	stainable enzyme activity	820:844	stainable enzyme activity	820:844	Analysis of wild (WT) type and eight mutant stable cell lines indicated that all mutants lacked stainable enzyme activity.
32214327	13	90	theme	intracellular	1877:1889	arg1	proteins					1910:1917	intracellular and secreted TRACP proteins	1877:1917	intracellular and secreted TRACP proteins	1877:1917	Western blot analysis with intracellular and secreted TRACP proteins also revealed similar observations indicating that mutant T89I is amply secreted as inactive protein.
32214327	3	91	theme	mutant	468:473	arg1	genes					475:479	ACP5 mutant genes	463:479	ACP5 mutant genes	463:479	ACP5 mutant genes were synthesized, transfected into human embryonic kidney (HEK-293) cells and stably expressing cell lines were established.
32214327	11	92	theme	culture	1618:1624	arg1	medium					1626:1631	the culture medium	1614:1631	the culture medium	1614:1631	None of the mutant proteins were proteolytically processed into isoform 5b intracellularly, and only three mutants were secreted in significant amounts into the culture medium as intact isoform 5a-like proteins.
32214327	0	93	theme	acid	72:75	arg1	phosphatase					77:87	tartrate-resistant acid phosphatase	53:87	tartrate-resistant acid phosphatase	53:87	Characterisation of ACP5 missense mutations encoding tartrate-resistant acid phosphatase associated with spondyloenchondrodysplasia.
32214327	3	94	dep	synthesized	486:496	arg1	transfected					499:509	transfected	499:509	transfected into human embryonic kidney (HEK-293) cells	499:553	ACP5 mutant genes were synthesized, transfected into human embryonic kidney (HEK-293) cells and stably expressing cell lines were established.
32214327	2	95	theme	reported	383:390	arg1	mutations					401:409	the eight reported missense mutations	373:409	the eight reported missense mutations in ACP5 associated with SPENCD on TRACP expression	373:460	This study was undertaken to characterize the eight reported missense mutations in ACP5 associated with SPENCD on TRACP expression.
32214327	7	96	theme	immune	974:979	arg1	patterns					992:999	variable immune reactivity patterns	965:999	variable immune reactivity patterns	965:999	The mutant TRACP proteins displayed variable immune reactivity patterns, and all drastically reduced enzymatic activity, revealing that there is no gross inhibition of TRACP biosynthesis by the mutations.
32214327	8	97	theme	enzyme	1191:1196	arg1	function					1198:1205	enzyme function	1191:1205	enzyme function	1191:1205	But they likely interfere with folding thereby impairing enzyme function.
32214327	6	98	theme	intact	895:900	arg1	proteins					902:909	intact proteins	895:909	intact proteins	895:909	All ACP5 mutant constructs were translated into intact proteins by HEK-293 cells.
32214327	12	99	theme	"	1841:1841	arg1	forms					1843:1847	"unfolded" forms	1832:1847	"unfolded" forms	1832:1847	Analysis of antibody reactivity patterns revealed that T89I and M264K mutant proteins retained some native conformation, whereas all others were in "denatured" or "unfolded" forms.
32214327	14	100	theme	sensitive	2065:2073	arg1	glycans					2075:2081	Endo-H sensitive glycans	2058:2081	Endo-H sensitive glycans	2058:2081	All mutant proteins were attacked by Endo-H sensitive glycans and none could be activated by proteolytic cleavage in vitro.
32214327	11	101	theme	isoform	1643:1649	arg1	mutants					1564:1570	only three mutants	1553:1570	only three mutants	1553:1570	None of the mutant proteins were proteolytically processed into isoform 5b intracellularly, and only three mutants were secreted in significant amounts into the culture medium as intact isoform 5a-like proteins.
32214327	11	101	theme	isoform	1643:1649	arg1	proteins					1659:1666	intact isoform 5a-like proteins	1636:1666	intact isoform 5a-like proteins	1636:1666	None of the mutant proteins were proteolytically processed into isoform 5b intracellularly, and only three mutants were secreted in significant amounts into the culture medium as intact isoform 5a-like proteins.
32214327	1	102	theme	inherited	259:267	arg1	spondyloenchondrodysplasia					294:319	spondyloenchondrodysplasia	294:319	spondyloenchondrodysplasia (SPENCD)	294:328	Biallelic mutations in ACP5, encoding tartrate-resistant acid phosphatase (TRACP), have recently been identified to cause the inherited immuno-osseous disorder, spondyloenchondrodysplasia (SPENCD).
32214327	1	102	theme	inherited	259:267	arg1	disorder					284:291	the inherited immuno-osseous disorder	255:291	the inherited immuno-osseous disorder	255:291	Biallelic mutations in ACP5, encoding tartrate-resistant acid phosphatase (TRACP), have recently been identified to cause the inherited immuno-osseous disorder, spondyloenchondrodysplasia (SPENCD).
32214327	3	103	theme	expressing	566:575	arg1	lines					582:586	stably expressing cell lines	559:586	stably expressing cell lines	559:586	ACP5 mutant genes were synthesized, transfected into human embryonic kidney (HEK-293) cells and stably expressing cell lines were established.
32214327	7	104	theme	TRACP	940:944	arg1	proteins					946:953	The mutant TRACP proteins	929:953	The mutant TRACP proteins	929:953	The mutant TRACP proteins displayed variable immune reactivity patterns, and all drastically reduced enzymatic activity, revealing that there is no gross inhibition of TRACP biosynthesis by the mutations.
32214327	10	105	theme	TRACP	1238:1242	arg1	5a					1244:1245	TRACP 5a	1238:1245	TRACP 5a	1238:1245	TRACP 5a is a less active monomeric enzyme (35kD), with the intact loop peptide and TRACP 5b is proteolytically cleaved highly active enzyme encompassing two subunits (23 kD and 16 kD) held together by disulfide bonds.
32214327	10	105	theme	TRACP	1238:1242	arg1	enzyme					1274:1279	a less active monomeric enzyme	1250:1279	a less active monomeric enzyme (35kD)	1250:1286	TRACP 5a is a less active monomeric enzyme (35kD), with the intact loop peptide and TRACP 5b is proteolytically cleaved highly active enzyme encompassing two subunits (23 kD and 16 kD) held together by disulfide bonds.
32702450	7	0	theme	complementary	1080:1092	arg1	XPS					1122:1124	XPS	1122:1124	XPS	1122:1124	For the former study, TOF-SIMS results were compared with a complementary surface analysis technique, XPS.
32702450	7	0	theme	complementary	1080:1092	arg1	technique					1111:1119	a complementary surface analysis technique	1078:1119	a complementary surface analysis technique	1078:1119	For the former study, TOF-SIMS results were compared with a complementary surface analysis technique, XPS.
32702450	5	1	theme	different	866:874	arg1	drugs					887:891	different inhalation drugs	866:891	different inhalation drugs	866:891	Two kinds of systems have been examined: 1) binary blends of lactose particles and coating agents, and 2) blends of different inhalation drugs with carrier lactose.
32702450	6	2	theme	surface	968:974	arg1	composition					976:986	the surface composition	964:986	the surface composition	964:986	For both kinds of systems, detailed insight into the surface composition and structure could be derived.
32702450	7	3	theme	surface	1094:1100	arg1	XPS					1122:1124	XPS	1122:1124	XPS	1122:1124	For the former study, TOF-SIMS results were compared with a complementary surface analysis technique, XPS.
32702450	7	3	theme	surface	1094:1100	arg1	technique					1111:1119	a complementary surface analysis technique	1078:1119	a complementary surface analysis technique	1078:1119	For the former study, TOF-SIMS results were compared with a complementary surface analysis technique, XPS.
32702450	2	4	from	case	342:345	arg1	critical					275:282	critical	275:282	critical	275:282	Surface properties are often critical to the behavior of powder formulations, especially in the case of dry powders for inhalation, as surface properties directly affect inter-particulate forces and, hence, the dispersibility of the formulation.
32702450	2	5	theme	powders	354:360	arg1	case					342:345	the case	338:345	the case of dry powders for inhalation	338:375	Surface properties are often critical to the behavior of powder formulations, especially in the case of dry powders for inhalation, as surface properties directly affect inter-particulate forces and, hence, the dispersibility of the formulation.
32702450	5	6	with	blends	856:861	arg1	lactose					906:912	carrier lactose	898:912	carrier lactose	898:912	Two kinds of systems have been examined: 1) binary blends of lactose particles and coating agents, and 2) blends of different inhalation drugs with carrier lactose.
32702450	2	7	theme	powder	303:308	arg1	formulations					310:321	powder formulations	303:321	powder formulations	303:321	Surface properties are often critical to the behavior of powder formulations, especially in the case of dry powders for inhalation, as surface properties directly affect inter-particulate forces and, hence, the dispersibility of the formulation.
32702450	2	8	theme	formulations	310:321	arg1	behavior					291:298	the behavior	287:298	the behavior of powder formulations	287:321	Surface properties are often critical to the behavior of powder formulations, especially in the case of dry powders for inhalation, as surface properties directly affect inter-particulate forces and, hence, the dispersibility of the formulation.
32702450	5	9	dep	blends	801:806	arg1	1					791:791	1	791:791	1	791:791	Two kinds of systems have been examined: 1) binary blends of lactose particles and coating agents, and 2) blends of different inhalation drugs with carrier lactose.
32702450	3	10	from	pixel	518:522	arg1	fit					528:530	fit	528:530	fit	528:530	The mass spectrum at each pixel was fit to a linear combination of reference spectra obtained by non-negatively constrained alternating least squares.
32702450	3	10	from	pixel	518:522	arg1	spectrum					501:508	The mass spectrum	492:508	The mass spectrum at each pixel	492:522	The mass spectrum at each pixel was fit to a linear combination of reference spectra obtained by non-negatively constrained alternating least squares.
32702450	3	11	theme	linear	537:542	arg1	combination					544:554	a linear combination	535:554	a linear combination of reference spectra obtained by non-negatively constrained alternating least squares	535:640	The mass spectrum at each pixel was fit to a linear combination of reference spectra obtained by non-negatively constrained alternating least squares.
32702450	5	12	theme	coating	833:839	arg1	agents					841:846	coating agents	833:846	coating agents	833:846	Two kinds of systems have been examined: 1) binary blends of lactose particles and coating agents, and 2) blends of different inhalation drugs with carrier lactose.
32702450	3	13	theme	mass	496:499	arg1	fit					528:530	fit	528:530	fit	528:530	The mass spectrum at each pixel was fit to a linear combination of reference spectra obtained by non-negatively constrained alternating least squares.
32702450	3	13	theme	mass	496:499	arg1	spectrum					501:508	The mass spectrum	492:508	The mass spectrum at each pixel	492:522	The mass spectrum at each pixel was fit to a linear combination of reference spectra obtained by non-negatively constrained alternating least squares.
32702450	1	14	theme	surface	177:183	arg1	composition					185:195	surface composition	177:195	surface composition	177:195	A multivariate TOF-SIMS methodology has been developed and applied to quantify surface composition and chemical distribution for dry powder blends.
32702450	5	15	theme	inhalation	876:885	arg1	drugs					887:891	different inhalation drugs	866:891	different inhalation drugs	866:891	Two kinds of systems have been examined: 1) binary blends of lactose particles and coating agents, and 2) blends of different inhalation drugs with carrier lactose.
32702450	0	16	theme	surface	42:48	arg1	structure					50:58	surface structure	42:58	surface structure	42:58	Quantification of surface composition and surface structure of inhalation powders using TOF-SIMS.
32702450	5	17	theme	binary	794:799	arg1	blends					801:806	binary blends	794:806	binary blends of lactose particles and coating agents	794:846	Two kinds of systems have been examined: 1) binary blends of lactose particles and coating agents, and 2) blends of different inhalation drugs with carrier lactose.
32702450	7	18	theme	TOF-SIMS	1042:1049	arg1	results					1051:1057	TOF-SIMS results	1042:1057	TOF-SIMS results	1042:1057	For the former study, TOF-SIMS results were compared with a complementary surface analysis technique, XPS.
32702450	3	19	theme	reference	559:567	arg1	spectra					569:575	reference spectra	559:575	reference spectra obtained by non-negatively constrained alternating least squares	559:640	The mass spectrum at each pixel was fit to a linear combination of reference spectra obtained by non-negatively constrained alternating least squares.
32702450	2	20	theme	surface	381:387	arg1	properties					389:398	surface properties	381:398	surface properties	381:398	Surface properties are often critical to the behavior of powder formulations, especially in the case of dry powders for inhalation, as surface properties directly affect inter-particulate forces and, hence, the dispersibility of the formulation.
32702450	6	21	theme	detailed	942:949	arg1	insight					951:957	detailed insight	942:957	detailed insight into the surface composition and structure	942:1000	For both kinds of systems, detailed insight into the surface composition and structure could be derived.
32702450	3	22	theme	spectra	569:575	arg1	combination					544:554	a linear combination	535:554	a linear combination of reference spectra obtained by non-negatively constrained alternating least squares	535:640	The mass spectrum at each pixel was fit to a linear combination of reference spectra obtained by non-negatively constrained alternating least squares.
32702450	1	23	theme	chemical	201:208	arg1	distribution					210:221	chemical distribution	201:221	chemical distribution	201:221	A multivariate TOF-SIMS methodology has been developed and applied to quantify surface composition and chemical distribution for dry powder blends.
32702450	0	24	theme	composition	26:36	arg1	Quantification					0:13	Quantification	0:13	Quantification of surface composition and surface structure of inhalation powders using TOF-SIMS.	0:96	Quantification of surface composition and surface structure of inhalation powders using TOF-SIMS.
32702450	5	25	with	blends	801:806	arg1	lactose					906:912	carrier lactose	898:912	carrier lactose	898:912	Two kinds of systems have been examined: 1) binary blends of lactose particles and coating agents, and 2) blends of different inhalation drugs with carrier lactose.
32702450	1	26	theme	multivariate	100:111	arg1	methodology					122:132	A multivariate TOF-SIMS methodology	98:132	A multivariate TOF-SIMS methodology	98:132	A multivariate TOF-SIMS methodology has been developed and applied to quantify surface composition and chemical distribution for dry powder blends.
32702450	5	27	theme	particles	819:827	arg1	blends					801:806	binary blends	794:806	binary blends of lactose particles and coating agents	794:846	Two kinds of systems have been examined: 1) binary blends of lactose particles and coating agents, and 2) blends of different inhalation drugs with carrier lactose.
32702450	5	27	theme	particles	819:827	arg1	blends					856:861	blends	856:861	2) blends of different inhalation drugs with carrier lactose	853:912	Two kinds of systems have been examined: 1) binary blends of lactose particles and coating agents, and 2) blends of different inhalation drugs with carrier lactose.
32702450	0	28	theme	surface	18:24	arg1	composition					26:36	surface composition	18:36	surface composition	18:36	Quantification of surface composition and surface structure of inhalation powders using TOF-SIMS.
32702450	6	29	theme	systems	933:939	arg1	kinds					924:928	both kinds	919:928	both kinds of systems	919:939	For both kinds of systems, detailed insight into the surface composition and structure could be derived.
32702450	1	30	theme	TOF-SIMS	113:120	arg1	methodology					122:132	A multivariate TOF-SIMS methodology	98:132	A multivariate TOF-SIMS methodology	98:132	A multivariate TOF-SIMS methodology has been developed and applied to quantify surface composition and chemical distribution for dry powder blends.
32702450	2	31	theme	formulation	479:489	arg1	forces					434:439	inter-particulate forces	416:439	inter-particulate forces	416:439	Surface properties are often critical to the behavior of powder formulations, especially in the case of dry powders for inhalation, as surface properties directly affect inter-particulate forces and, hence, the dispersibility of the formulation.
32702450	2	31	theme	formulation	479:489	arg1	dispersibility					457:470	the dispersibility	453:470	the dispersibility of the formulation	453:489	Surface properties are often critical to the behavior of powder formulations, especially in the case of dry powders for inhalation, as surface properties directly affect inter-particulate forces and, hence, the dispersibility of the formulation.
32702450	4	32	theme	other	712:716	arg1	features					724:731	other image features	712:731	other image features	712:731	From the pixel compositions, average surface coverage and a range of other image features were calculated.
32702450	1	33	theme	dry	227:229	arg1	blends					238:243	dry powder blends	227:243	dry powder blends	227:243	A multivariate TOF-SIMS methodology has been developed and applied to quantify surface composition and chemical distribution for dry powder blends.
32702450	2	34	from	critical	275:282	arg1	case					342:345	the case	338:345	the case of dry powders for inhalation	338:375	Surface properties are often critical to the behavior of powder formulations, especially in the case of dry powders for inhalation, as surface properties directly affect inter-particulate forces and, hence, the dispersibility of the formulation.
32702450	2	35	theme	inter-particulate	416:432	arg1	forces					434:439	inter-particulate forces	416:439	inter-particulate forces	416:439	Surface properties are often critical to the behavior of powder formulations, especially in the case of dry powders for inhalation, as surface properties directly affect inter-particulate forces and, hence, the dispersibility of the formulation.
32702450	4	36	theme	average	672:678	arg1	coverage					688:695	average surface coverage	672:695	average surface coverage	672:695	From the pixel compositions, average surface coverage and a range of other image features were calculated.
32702450	7	37	theme	former	1028:1033	arg1	study					1035:1039	the former study	1024:1039	the former study	1024:1039	For the former study, TOF-SIMS results were compared with a complementary surface analysis technique, XPS.
32702450	2	38	theme	Surface	246:252	arg1	properties					254:263	Surface properties	246:263	Surface properties	246:263	Surface properties are often critical to the behavior of powder formulations, especially in the case of dry powders for inhalation, as surface properties directly affect inter-particulate forces and, hence, the dispersibility of the formulation.
32702450	5	39	theme	agents	841:846	arg1	blends					801:806	binary blends	794:806	binary blends of lactose particles and coating agents	794:846	Two kinds of systems have been examined: 1) binary blends of lactose particles and coating agents, and 2) blends of different inhalation drugs with carrier lactose.
32702450	5	39	theme	agents	841:846	arg1	blends					856:861	blends	856:861	2) blends of different inhalation drugs with carrier lactose	853:912	Two kinds of systems have been examined: 1) binary blends of lactose particles and coating agents, and 2) blends of different inhalation drugs with carrier lactose.
32702450	1	40	theme	powder	231:236	arg1	blends					238:243	dry powder blends	227:243	dry powder blends	227:243	A multivariate TOF-SIMS methodology has been developed and applied to quantify surface composition and chemical distribution for dry powder blends.
32702450	7	41	theme	analysis	1102:1109	arg1	XPS					1122:1124	XPS	1122:1124	XPS	1122:1124	For the former study, TOF-SIMS results were compared with a complementary surface analysis technique, XPS.
32702450	7	41	theme	analysis	1102:1109	arg1	technique					1111:1119	a complementary surface analysis technique	1078:1119	a complementary surface analysis technique	1078:1119	For the former study, TOF-SIMS results were compared with a complementary surface analysis technique, XPS.
32702450	5	42	theme	systems	763:769	arg1	kinds					754:758	Two kinds	750:758	Two kinds of systems	750:769	Two kinds of systems have been examined: 1) binary blends of lactose particles and coating agents, and 2) blends of different inhalation drugs with carrier lactose.
32702450	2	43	theme	dry	350:352	arg1	powders					354:360	dry powders	350:360	dry powders for inhalation	350:375	Surface properties are often critical to the behavior of powder formulations, especially in the case of dry powders for inhalation, as surface properties directly affect inter-particulate forces and, hence, the dispersibility of the formulation.
32702450	0	44	theme	structure	50:58	arg1	Quantification					0:13	Quantification	0:13	Quantification of surface composition and surface structure of inhalation powders using TOF-SIMS.	0:96	Quantification of surface composition and surface structure of inhalation powders using TOF-SIMS.
32702450	4	45	theme	surface	680:686	arg1	coverage					688:695	average surface coverage	672:695	average surface coverage	672:695	From the pixel compositions, average surface coverage and a range of other image features were calculated.
32702450	4	46	theme	image	718:722	arg1	features					724:731	other image features	712:731	other image features	712:731	From the pixel compositions, average surface coverage and a range of other image features were calculated.
32702450	5	47	theme	carrier	898:904	arg1	lactose					906:912	carrier lactose	898:912	carrier lactose	898:912	Two kinds of systems have been examined: 1) binary blends of lactose particles and coating agents, and 2) blends of different inhalation drugs with carrier lactose.
32702450	5	48	dep	blends	856:861	arg1	2					853:853	2	853:853	2	853:853	Two kinds of systems have been examined: 1) binary blends of lactose particles and coating agents, and 2) blends of different inhalation drugs with carrier lactose.
32702450	5	49	theme	drugs	887:891	arg1	blends					801:806	binary blends	794:806	binary blends of lactose particles and coating agents	794:846	Two kinds of systems have been examined: 1) binary blends of lactose particles and coating agents, and 2) blends of different inhalation drugs with carrier lactose.
32702450	5	49	theme	drugs	887:891	arg1	blends					856:861	blends	856:861	2) blends of different inhalation drugs with carrier lactose	853:912	Two kinds of systems have been examined: 1) binary blends of lactose particles and coating agents, and 2) blends of different inhalation drugs with carrier lactose.
32702450	5	50	theme	lactose	811:817	arg1	particles					819:827	lactose particles	811:827	lactose particles	811:827	Two kinds of systems have been examined: 1) binary blends of lactose particles and coating agents, and 2) blends of different inhalation drugs with carrier lactose.
32702450	4	51	theme	features	724:731	arg1	range					703:707	a range	701:707	a range of other image features	701:731	From the pixel compositions, average surface coverage and a range of other image features were calculated.
32702450	4	51	theme	features	724:731	arg1	coverage					688:695	average surface coverage	672:695	average surface coverage	672:695	From the pixel compositions, average surface coverage and a range of other image features were calculated.
32702450	0	52	theme	powders	74:80	arg1	composition					26:36	surface composition	18:36	surface composition	18:36	Quantification of surface composition and surface structure of inhalation powders using TOF-SIMS.
32702450	0	52	theme	powders	74:80	arg1	structure					50:58	surface structure	42:58	surface structure	42:58	Quantification of surface composition and surface structure of inhalation powders using TOF-SIMS.
32702450	4	53	theme	pixel	652:656	arg1	compositions					658:669	the pixel compositions	648:669	the pixel compositions	648:669	From the pixel compositions, average surface coverage and a range of other image features were calculated.
32702450	0	54	theme	inhalation	63:72	arg1	powders					74:80	inhalation powders	63:80	inhalation powders using TOF-SIMS	63:95	Quantification of surface composition and surface structure of inhalation powders using TOF-SIMS.
32702450	3	55	theme	least	628:632	arg1	squares					634:640	least squares	628:640	least squares	628:640	The mass spectrum at each pixel was fit to a linear combination of reference spectra obtained by non-negatively constrained alternating least squares.
32526304	5	0	theme	SEM	727:729	arg1	measurements					768:779	SEM, AFM, and particle size distribution measurements	727:779	SEM, AFM, and particle size distribution measurements	727:779	SEM, AFM, and particle size distribution measurements showed that HSP-W had relatively clustered spherical shape with different sizes from nanoscale to micrometer.
32526304	0	1	from	purification	11:22	arg1	cells					175:179	LPS-stimulated THP-1 cells	154:179	LPS-stimulated THP-1 cells	154:179	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	1	from	purification	11:22	arg1	Huangshui					75:83	Huangshui	75:83	Huangshui	75:83	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	2	theme	THP-1	169:173	arg1	cells					175:179	LPS-stimulated THP-1 cells	154:179	LPS-stimulated THP-1 cells	154:179	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	4	3	theme	monosaccharide	664:677	arg1	composition					679:689	monosaccharide composition	664:689	monosaccharide composition	664:689	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	4	4	theme	linked	531:536	arg1	backbone					547:554	a 1,4 linked α-D-Glcp backbone	525:554	a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses	525:724	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	7	5	theme	cytokines	1163:1171	arg1	expressions					1142:1152	mRNA and protein expressions	1125:1152	mRNA and protein expressions of these cytokines	1125:1171	Meanwhile, HSP-W treatment markedly enhanced mRNA and protein expressions of these cytokines.
32526304	0	6	theme	LPS-stimulated	154:167	arg1	cells					175:179	LPS-stimulated THP-1 cells	154:179	LPS-stimulated THP-1 cells	154:179	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	7	from	activity	142:149	arg1	cells					175:179	LPS-stimulated THP-1 cells	154:179	LPS-stimulated THP-1 cells	154:179	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	7	from	activity	142:149	arg1	Huangshui					75:83	Huangshui	75:83	Huangshui	75:83	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	8	from	Isolation	0:8	arg1	cells					175:179	LPS-stimulated THP-1 cells	154:179	LPS-stimulated THP-1 cells	154:179	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	8	from	Isolation	0:8	arg1	Huangshui					75:83	Huangshui	75:83	Huangshui	75:83	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	5	9	from	nanoscale	866:874	arg1	sizes					855:859	different sizes	845:859	different sizes from nanoscale to micrometer	845:888	SEM, AFM, and particle size distribution measurements showed that HSP-W had relatively clustered spherical shape with different sizes from nanoscale to micrometer.
32526304	7	10	theme	protein	1134:1140	arg1	expressions					1142:1152	mRNA and protein expressions	1125:1152	mRNA and protein expressions of these cytokines	1125:1171	Meanwhile, HSP-W treatment markedly enhanced mRNA and protein expressions of these cytokines.
32526304	6	11	theme	pinocytic	1028:1036	arg1	capacities					1053:1062	pinocytic and phagocytic capacities	1028:1062	pinocytic and phagocytic capacities of THP-1 cells	1028:1077	Additionally, HSP-W significantly induced NO and ROS production as well as the release of IL-1β, IFN-γ, TNF-α, and IL-6, and upregulated pinocytic and phagocytic capacities of THP-1 cells.
32526304	4	12	theme	α-D-Glcp	538:545	arg1	backbone					547:554	a 1,4 linked α-D-Glcp backbone	525:554	a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses	525:724	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	6	13	theme	TNF-α	995:999	arg1	release					970:976	the release	966:976	NO and ROS production as well as the release of IL-1β, IFN-γ, TNF-α, and IL-6	933:1009	Additionally, HSP-W significantly induced NO and ROS production as well as the release of IL-1β, IFN-γ, TNF-α, and IL-6, and upregulated pinocytic and phagocytic capacities of THP-1 cells.
32526304	6	13	theme	TNF-α	995:999	arg1	production					944:953	NO and ROS production	933:953	production	944:953	Additionally, HSP-W significantly induced NO and ROS production as well as the release of IL-1β, IFN-γ, TNF-α, and IL-6, and upregulated pinocytic and phagocytic capacities of THP-1 cells.
32526304	3	14	theme	water-eluted	409:420	arg1	HSP-W					441:445	HSP-W	441:445	HSP-W	441:445	In this study, a novel water-eluted HS polysaccharide, HSP-W, was identified as an α-D-glucan with a Mw of 166.00 kDa.
32526304	3	14	theme	water-eluted	409:420	arg1	α-D-glucan					469:478	an α-D-glucan	466:478	an α-D-glucan with a Mw of 166.00 kDa	466:502	In this study, a novel water-eluted HS polysaccharide, HSP-W, was identified as an α-D-glucan with a Mw of 166.00 kDa.
32526304	3	14	theme	water-eluted	409:420	arg1	polysaccharide					425:438	a novel water-eluted HS polysaccharide	401:438	a novel water-eluted HS polysaccharide	401:438	In this study, a novel water-eluted HS polysaccharide, HSP-W, was identified as an α-D-glucan with a Mw of 166.00 kDa.
32526304	6	15	theme	IFN-γ	988:992	arg1	release					970:976	the release	966:976	NO and ROS production as well as the release of IL-1β, IFN-γ, TNF-α, and IL-6	933:1009	Additionally, HSP-W significantly induced NO and ROS production as well as the release of IL-1β, IFN-γ, TNF-α, and IL-6, and upregulated pinocytic and phagocytic capacities of THP-1 cells.
32526304	6	15	theme	IFN-γ	988:992	arg1	production					944:953	NO and ROS production	933:953	production	944:953	Additionally, HSP-W significantly induced NO and ROS production as well as the release of IL-1β, IFN-γ, TNF-α, and IL-6, and upregulated pinocytic and phagocytic capacities of THP-1 cells.
32526304	0	16	from	characterization	35:50	arg1	cells					175:179	LPS-stimulated THP-1 cells	154:179	LPS-stimulated THP-1 cells	154:179	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	16	from	characterization	35:50	arg1	Huangshui					75:83	Huangshui	75:83	Huangshui	75:83	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	3	17	theme	166.00 kDa	493:502	arg1	Mw					487:488	a Mw	485:488	a Mw of 166.00 kDa	485:502	In this study, a novel water-eluted HS polysaccharide, HSP-W, was identified as an α-D-glucan with a Mw of 166.00 kDa.
32526304	3	18	theme	HS	422:423	arg1	HSP-W					441:445	HSP-W	441:445	HSP-W	441:445	In this study, a novel water-eluted HS polysaccharide, HSP-W, was identified as an α-D-glucan with a Mw of 166.00 kDa.
32526304	3	18	theme	HS	422:423	arg1	α-D-glucan					469:478	an α-D-glucan	466:478	an α-D-glucan with a Mw of 166.00 kDa	466:502	In this study, a novel water-eluted HS polysaccharide, HSP-W, was identified as an α-D-glucan with a Mw of 166.00 kDa.
32526304	3	18	theme	HS	422:423	arg1	polysaccharide					425:438	a novel water-eluted HS polysaccharide	401:438	a novel water-eluted HS polysaccharide	401:438	In this study, a novel water-eluted HS polysaccharide, HSP-W, was identified as an α-D-glucan with a Mw of 166.00 kDa.
32526304	5	19	theme	size	750:753	arg1	distribution					755:766	particle size distribution	741:766	particle size distribution	741:766	SEM, AFM, and particle size distribution measurements showed that HSP-W had relatively clustered spherical shape with different sizes from nanoscale to micrometer.
32526304	6	20	theme	NO	933:934	arg1	production					944:953	NO and ROS production	933:953	production	944:953	Additionally, HSP-W significantly induced NO and ROS production as well as the release of IL-1β, IFN-γ, TNF-α, and IL-6, and upregulated pinocytic and phagocytic capacities of THP-1 cells.
32526304	5	21	contain	had	799:801	arg2	shape					834:838	relatively clustered spherical shape	803:838	relatively clustered spherical shape	803:838	SEM, AFM, and particle size distribution measurements showed that HSP-W had relatively clustered spherical shape with different sizes from nanoscale to micrometer.
32526304	5	21	contain	had	799:801	arg1	HSP-W					793:797	HSP-W	793:797	HSP-W	793:797	SEM, AFM, and particle size distribution measurements showed that HSP-W had relatively clustered spherical shape with different sizes from nanoscale to micrometer.
32526304	3	22	with	α-D-glucan	469:478	arg1	Mw					487:488	a Mw	485:488	a Mw of 166.00 kDa	485:502	In this study, a novel water-eluted HS polysaccharide, HSP-W, was identified as an α-D-glucan with a Mw of 166.00 kDa.
32526304	6	23	theme	IL-6	1006:1009	arg1	release					970:976	the release	966:976	NO and ROS production as well as the release of IL-1β, IFN-γ, TNF-α, and IL-6	933:1009	Additionally, HSP-W significantly induced NO and ROS production as well as the release of IL-1β, IFN-γ, TNF-α, and IL-6, and upregulated pinocytic and phagocytic capacities of THP-1 cells.
32526304	6	23	theme	IL-6	1006:1009	arg1	production					944:953	NO and ROS production	933:953	production	944:953	Additionally, HSP-W significantly induced NO and ROS production as well as the release of IL-1β, IFN-γ, TNF-α, and IL-6, and upregulated pinocytic and phagocytic capacities of THP-1 cells.
32526304	5	24	theme	distribution	755:766	arg1	measurements					768:779	SEM, AFM, and particle size distribution measurements	727:779	SEM, AFM, and particle size distribution measurements	727:779	SEM, AFM, and particle size distribution measurements showed that HSP-W had relatively clustered spherical shape with different sizes from nanoscale to micrometer.
32526304	1	25	theme	aroma	257:261	arg1	compounds					263:271	aroma compounds	257:271	aroma compounds	257:271	Huangshui (HS) has attracted considerable attention for the utilization of aroma compounds and microorganisms containing presently.
32526304	8	26	contain	has	1186:1188	arg2	agent					1236:1240	an immunostimulatory agent	1215:1240	an immunostimulatory agent	1215:1240	HS probably has potential application as an immunostimulatory agent.
32526304	8	26	contain	has	1186:1188	arg2	application					1200:1210	potential application	1190:1210	potential application	1190:1210	HS probably has potential application as an immunostimulatory agent.
32526304	8	26	contain	has	1186:1188	arg1	HS					1174:1175	HS	1174:1175	HS	1174:1175	HS probably has potential application as an immunostimulatory agent.
32526304	5	27	theme	clustered	814:822	arg1	shape					834:838	relatively clustered spherical shape	803:838	relatively clustered spherical shape	803:838	SEM, AFM, and particle size distribution measurements showed that HSP-W had relatively clustered spherical shape with different sizes from nanoscale to micrometer.
32526304	3	28	theme	novel	403:407	arg1	HSP-W					441:445	HSP-W	441:445	HSP-W	441:445	In this study, a novel water-eluted HS polysaccharide, HSP-W, was identified as an α-D-glucan with a Mw of 166.00 kDa.
32526304	3	28	theme	novel	403:407	arg1	α-D-glucan					469:478	an α-D-glucan	466:478	an α-D-glucan with a Mw of 166.00 kDa	466:502	In this study, a novel water-eluted HS polysaccharide, HSP-W, was identified as an α-D-glucan with a Mw of 166.00 kDa.
32526304	3	28	theme	novel	403:407	arg1	polysaccharide					425:438	a novel water-eluted HS polysaccharide	401:438	a novel water-eluted HS polysaccharide	401:438	In this study, a novel water-eluted HS polysaccharide, HSP-W, was identified as an α-D-glucan with a Mw of 166.00 kDa.
32526304	1	29	theme	compounds	263:271	arg1	utilization					242:252	the utilization	238:252	the utilization of aroma compounds and microorganisms containing presently	238:311	Huangshui (HS) has attracted considerable attention for the utilization of aroma compounds and microorganisms containing presently.
32526304	0	30	theme	Baijiu	109:114	arg1	purification					11:22	purification	11:22	purification	11:22	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	30	theme	Baijiu	109:114	arg1	activity					142:149	its immunomodulatory activity	121:149	its immunomodulatory activity in LPS-stimulated THP-1 cells	121:179	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	30	theme	Baijiu	109:114	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	30	theme	Baijiu	109:114	arg1	byproduct					88:96	a byproduct	86:96	a byproduct of Chinese Baijiu	86:114	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	30	theme	Baijiu	109:114	arg1	characterization					35:50	structure characterization	25:50	structure characterization of a novel glucan from Huangshui	25:83	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	4	31	theme	NMR	713:715	arg1	analyses					717:724	NMR analyses	713:724	NMR analyses	713:724	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	0	32	theme	Chinese	101:107	arg1	Baijiu					109:114	Chinese Baijiu	101:114	Chinese Baijiu	101:114	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	33	from	Huangshui	75:83	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	33	from	Huangshui	75:83	arg1	byproduct					88:96	a byproduct	86:96	a byproduct of Chinese Baijiu	86:114	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	33	from	Huangshui	75:83	arg1	purification					11:22	purification	11:22	purification	11:22	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	33	from	Huangshui	75:83	arg1	activity					142:149	its immunomodulatory activity	121:149	its immunomodulatory activity in LPS-stimulated THP-1 cells	121:179	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	33	from	Huangshui	75:83	arg1	glucan					63:68	a novel glucan	55:68	a novel glucan from Huangshui	55:83	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	33	from	Huangshui	75:83	arg1	characterization					35:50	structure characterization	25:50	structure characterization of a novel glucan from Huangshui	25:83	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	4	34	from	O-6	581:583	arg1	substitution					565:576	the substitution	561:576	the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses	561:724	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	4	35	theme	β-Glc-1→	647:654	arg1	β-Glc-1→					647:654	β-Glc-1→	647:654	β-Glc-1→	647:654	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	4	35	theme	β-Glc-1→	647:654	arg1	residue					610:616	1,6-linked α-D-Glcp residue	590:616	1,6-linked α-D-Glcp residue	590:616	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	4	35	theme	β-Glc-1→	647:654	arg1	terminal					635:642	non-reducing terminal	622:642	non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses	622:724	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	5	36	theme	AFM	732:734	arg1	measurements					768:779	SEM, AFM, and particle size distribution measurements	727:779	SEM, AFM, and particle size distribution measurements	727:779	SEM, AFM, and particle size distribution measurements showed that HSP-W had relatively clustered spherical shape with different sizes from nanoscale to micrometer.
32526304	1	37	theme	microorganisms	277:290	arg1	utilization					242:252	the utilization	238:252	the utilization of aroma compounds and microorganisms containing presently	238:311	Huangshui (HS) has attracted considerable attention for the utilization of aroma compounds and microorganisms containing presently.
32526304	7	38	theme	mRNA	1125:1128	arg1	expressions					1142:1152	mRNA and protein expressions	1125:1152	mRNA and protein expressions of these cytokines	1125:1171	Meanwhile, HSP-W treatment markedly enhanced mRNA and protein expressions of these cytokines.
32526304	4	39	theme	non-reducing	622:633	arg1	terminal					635:642	non-reducing terminal	622:642	non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses	622:724	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	4	40	theme	α-D-Glcp	601:608	arg1	β-Glc-1→					647:654	β-Glc-1→	647:654	β-Glc-1→	647:654	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	4	40	theme	α-D-Glcp	601:608	arg1	residue					610:616	1,6-linked α-D-Glcp residue	590:616	1,6-linked α-D-Glcp residue	590:616	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	0	41	theme	structure	25:33	arg1	characterization					35:50	structure characterization	25:50	structure characterization of a novel glucan from Huangshui	25:83	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	7	42	theme	HSP-W	1091:1095	arg1	treatment					1097:1105	HSP-W treatment	1091:1105	HSP-W treatment	1091:1105	Meanwhile, HSP-W treatment markedly enhanced mRNA and protein expressions of these cytokines.
32526304	4	43	dep	linked	531:536	arg1	1,4					527:529	1,4	527:529	1,4	527:529	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	4	44	theme	1,6-linked	590:599	arg1	β-Glc-1→					647:654	β-Glc-1→	647:654	β-Glc-1→	647:654	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	4	44	theme	1,6-linked	590:599	arg1	residue					610:616	1,6-linked α-D-Glcp residue	590:616	1,6-linked α-D-Glcp residue	590:616	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	5	45	theme	different	845:853	arg1	sizes					855:859	different sizes	845:859	different sizes from nanoscale to micrometer	845:888	SEM, AFM, and particle size distribution measurements showed that HSP-W had relatively clustered spherical shape with different sizes from nanoscale to micrometer.
32526304	0	46	theme	immunomodulatory	125:140	arg1	activity					142:149	its immunomodulatory activity	121:149	its immunomodulatory activity in LPS-stimulated THP-1 cells	121:179	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	6	47	theme	ROS	940:942	arg1	production					944:953	NO and ROS production	933:953	production	944:953	Additionally, HSP-W significantly induced NO and ROS production as well as the release of IL-1β, IFN-γ, TNF-α, and IL-6, and upregulated pinocytic and phagocytic capacities of THP-1 cells.
32526304	8	48	theme	potential	1190:1198	arg1	application					1200:1210	potential application	1190:1210	potential application	1190:1210	HS probably has potential application as an immunostimulatory agent.
32526304	8	48	theme	potential	1190:1198	arg1	agent					1236:1240	an immunostimulatory agent	1215:1240	an immunostimulatory agent	1215:1240	HS probably has potential application as an immunostimulatory agent.
32526304	2	49	from	polysaccharides	363:377	arg1	HS					382:383	HS	382:383	HS	382:383	However, little work has been done on biological polysaccharides in HS.
32526304	6	50	theme	cells	1073:1077	arg1	capacities					1053:1062	pinocytic and phagocytic capacities	1028:1062	pinocytic and phagocytic capacities of THP-1 cells	1028:1077	Additionally, HSP-W significantly induced NO and ROS production as well as the release of IL-1β, IFN-γ, TNF-α, and IL-6, and upregulated pinocytic and phagocytic capacities of THP-1 cells.
32526304	5	51	theme	particle	741:748	arg1	distribution					755:766	particle size distribution	741:766	particle size distribution	741:766	SEM, AFM, and particle size distribution measurements showed that HSP-W had relatively clustered spherical shape with different sizes from nanoscale to micrometer.
32526304	6	52	theme	THP-1	1067:1071	arg1	cells					1073:1077	THP-1 cells	1067:1077	THP-1 cells	1067:1077	Additionally, HSP-W significantly induced NO and ROS production as well as the release of IL-1β, IFN-γ, TNF-α, and IL-6, and upregulated pinocytic and phagocytic capacities of THP-1 cells.
32526304	0	53	from	byproduct	88:96	arg1	cells					175:179	LPS-stimulated THP-1 cells	154:179	LPS-stimulated THP-1 cells	154:179	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	53	from	byproduct	88:96	arg1	Huangshui					75:83	Huangshui	75:83	Huangshui	75:83	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	4	54	with	backbone	547:554	arg1	substitution					565:576	the substitution	561:576	the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses	561:724	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	2	55	theme	little	323:328	arg1	work					330:333	little work	323:333	little work	323:333	However, little work has been done on biological polysaccharides in HS.
32526304	8	56	theme	immunostimulatory	1218:1234	arg1	application					1200:1210	potential application	1190:1210	potential application	1190:1210	HS probably has potential application as an immunostimulatory agent.
32526304	8	56	theme	immunostimulatory	1218:1234	arg1	agent					1236:1240	an immunostimulatory agent	1215:1240	an immunostimulatory agent	1215:1240	HS probably has potential application as an immunostimulatory agent.
32526304	4	57	link	linked	531:536	arg1	backbone					547:554	a 1,4 linked α-D-Glcp backbone	525:554	a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses	525:724	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	4	58	link	1,6-linked	590:599	arg1	β-Glc-1→					647:654	β-Glc-1→	647:654	β-Glc-1→	647:654	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	4	58	link	1,6-linked	590:599	arg1	residue					610:616	1,6-linked α-D-Glcp residue	590:616	1,6-linked α-D-Glcp residue	590:616	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	6	59	theme	phagocytic	1042:1051	arg1	capacities					1053:1062	pinocytic and phagocytic capacities	1028:1062	pinocytic and phagocytic capacities of THP-1 cells	1028:1077	Additionally, HSP-W significantly induced NO and ROS production as well as the release of IL-1β, IFN-γ, TNF-α, and IL-6, and upregulated pinocytic and phagocytic capacities of THP-1 cells.
32526304	6	60	theme	IL-1β	981:985	arg1	release					970:976	the release	966:976	NO and ROS production as well as the release of IL-1β, IFN-γ, TNF-α, and IL-6	933:1009	Additionally, HSP-W significantly induced NO and ROS production as well as the release of IL-1β, IFN-γ, TNF-α, and IL-6, and upregulated pinocytic and phagocytic capacities of THP-1 cells.
32526304	6	60	theme	IL-1β	981:985	arg1	production					944:953	NO and ROS production	933:953	production	944:953	Additionally, HSP-W significantly induced NO and ROS production as well as the release of IL-1β, IFN-γ, TNF-α, and IL-6, and upregulated pinocytic and phagocytic capacities of THP-1 cells.
32526304	0	61	theme	glucan	63:68	arg1	purification					11:22	purification	11:22	purification	11:22	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	61	theme	glucan	63:68	arg1	activity					142:149	its immunomodulatory activity	121:149	its immunomodulatory activity in LPS-stimulated THP-1 cells	121:179	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	61	theme	glucan	63:68	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	61	theme	glucan	63:68	arg1	byproduct					88:96	a byproduct	86:96	a byproduct of Chinese Baijiu	86:114	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	0	61	theme	glucan	63:68	arg1	characterization					35:50	structure characterization	25:50	structure characterization of a novel glucan from Huangshui	25:83	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32526304	4	62	with	O-6	581:583	arg1	β-Glc-1→					647:654	β-Glc-1→	647:654	β-Glc-1→	647:654	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	4	62	with	O-6	581:583	arg1	residue					610:616	1,6-linked α-D-Glcp residue	590:616	1,6-linked α-D-Glcp residue	590:616	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	4	62	with	O-6	581:583	arg1	terminal					635:642	non-reducing terminal	622:642	non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses	622:724	It was consisted of a 1,4 linked α-D-Glcp backbone with the substitution at O-6 with 1,6-linked α-D-Glcp residue and non-reducing terminal of β-Glc-1→ through monosaccharide composition, IR, methylation, and NMR analyses.
32526304	2	63	theme	biological	352:361	arg1	polysaccharides					363:377	biological polysaccharides	352:377	biological polysaccharides in HS	352:383	However, little work has been done on biological polysaccharides in HS.
32526304	5	64	theme	spherical	824:832	arg1	shape					834:838	relatively clustered spherical shape	803:838	relatively clustered spherical shape	803:838	SEM, AFM, and particle size distribution measurements showed that HSP-W had relatively clustered spherical shape with different sizes from nanoscale to micrometer.
32526304	1	65	theme	considerable	211:222	arg1	attention					224:232	considerable attention	211:232	considerable attention	211:232	Huangshui (HS) has attracted considerable attention for the utilization of aroma compounds and microorganisms containing presently.
32526304	0	66	theme	novel	57:61	arg1	glucan					63:68	a novel glucan	55:68	a novel glucan from Huangshui	55:83	Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of Chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells.
32450234	9	0	theme	Sephadex	909:916	arg1	column					924:929	Sephadex G-100 column	909:929	Sephadex G-100 column	909:929	MATERIALS AND METHODS PCPs were prepared by water extraction and alcohol precipitation method and purified with DEAE-52 cellulose column and Sephadex G-100 column.
32450234	2	1	theme	vomiting	278:285	arg1	treatment					255:263	the treatment	251:263	the treatment of diarrhea, vomiting, nausea and fever	251:303	is widely used in China as a traditional Chinese medicine for the treatment of diarrhea, vomiting, nausea and fever.
32450234	21	2	theme	PCPs	2246:2249	arg1	effects					2235:2241	the anti-oxidative effects	2216:2241	the anti-oxidative effects of PCPs	2216:2249	Furthermore, the anti-oxidative effects of PCPs play important roles in their antiviral activities against PEDV.
32450234	2	3	theme	diarrhea	268:275	arg1	treatment					255:263	the treatment	251:263	the treatment of diarrhea, vomiting, nausea and fever	251:303	is widely used in China as a traditional Chinese medicine for the treatment of diarrhea, vomiting, nausea and fever.
32450234	21	4	theme	important	2256:2264	arg1	roles					2266:2270	important roles	2256:2270	important roles	2256:2270	Furthermore, the anti-oxidative effects of PCPs play important roles in their antiviral activities against PEDV.
32450234	2	5	theme	fever	299:303	arg1	treatment					255:263	the treatment	251:263	the treatment of diarrhea, vomiting, nausea and fever	251:303	is widely used in China as a traditional Chinese medicine for the treatment of diarrhea, vomiting, nausea and fever.
32450234	8	6	theme	study	674:678	arg1	purpose					655:661	The purpose	651:661	The purpose of present study	651:678	AIM OF THE STUDY The purpose of present study is to investigate the structural characterization and the anti-PEDV activities of PCPs.
32450234	10	7	theme	infrared	1007:1014	arg1	spectrum					1016:1023	the infrared spectrum	1003:1023	the infrared spectrum	1003:1023	Then, the structural characterization of the polysaccharides including the infrared spectrum, molecular weight and monosaccharide composition were analyzed.
32450234	2	8	theme	nausea	288:293	arg1	treatment					255:263	the treatment	251:263	the treatment of diarrhea, vomiting, nausea and fever	251:303	is widely used in China as a traditional Chinese medicine for the treatment of diarrhea, vomiting, nausea and fever.
32450234	12	9	theme	PCR	1321:1323	arg1	method					1325:1330	real-time PCR method	1311:1330	real-time PCR method	1311:1330	Additionally, the effects of PCPs on PEDV adsorption, penetration and replication were analyzed by real-time PCR method.
32450234	19	10	theme	Pogostemon	2090:2099	arg1	Benth					2117:2121	Pogostemon cablin (Blanco) Benth	2090:2121	Pogostemon cablin (Blanco) Benth	2090:2121	CONCLUSIONS The treatment effect of Pogostemon cablin (Blanco) Benth.
32450234	2	11	theme	Chinese	230:236	arg1	medicine					238:245	a traditional Chinese medicine	216:245	a traditional Chinese medicine for the treatment of diarrhea, vomiting, nausea and fever	216:303	is widely used in China as a traditional Chinese medicine for the treatment of diarrhea, vomiting, nausea and fever.
32450234	15	12	theme	galacturonic	1773:1784	arg1	acid					1786:1789	galacturonic acid	1773:1789	galacturonic acid	1773:1789	PCP1.1, PCP1.2 and PCP2.1 were composed of fucose, arabinose, galactose, glucose, mannose, galacturonic acid and glucuronic acid; and PCP2.2 was composed of arabinose, galactose, glucose, galacturonic acid and glucuronic acid.
32450234	15	12	theme	galacturonic	1773:1784	arg1	arabinose					1742:1750	arabinose	1742:1750	arabinose	1742:1750	PCP1.1, PCP1.2 and PCP2.1 were composed of fucose, arabinose, galactose, glucose, mannose, galacturonic acid and glucuronic acid; and PCP2.2 was composed of arabinose, galactose, glucose, galacturonic acid and glucuronic acid.
32450234	9	13	theme	water	812:816	arg1	extraction					818:827	water extraction	812:827	water extraction	812:827	MATERIALS AND METHODS PCPs were prepared by water extraction and alcohol precipitation method and purified with DEAE-52 cellulose column and Sephadex G-100 column.
32450234	7	14	contain	has	557:559	arg1	polysaccharides					534:548	Pogostemon cablin polysaccharides	516:548	Pogostemon cablin polysaccharides (PCPs)	516:555	But it is not known whether Pogostemon cablin polysaccharides (PCPs) has the antiviral activities against porcine epidemic diarrhea virus (PEDV).
32450234	7	14	contain	has	557:559	arg1	PCPs					551:554	PCPs	551:554	PCPs	551:554	But it is not known whether Pogostemon cablin polysaccharides (PCPs) has the antiviral activities against porcine epidemic diarrhea virus (PEDV).
32450234	7	14	contain	has	557:559	arg2	activities					575:584	the antiviral activities	561:584	the antiviral activities against porcine epidemic diarrhea virus (PEDV)	561:631	But it is not known whether Pogostemon cablin polysaccharides (PCPs) has the antiviral activities against porcine epidemic diarrhea virus (PEDV).
32450234	14	15	dep	RESULTS	1453:1459	arg1	polysaccharides					1466:1480	Four polysaccharides	1461:1480	RESULTS Four polysaccharides	1453:1480	RESULTS Four polysaccharides were obtained and named as PCP1.1 (31.3 kDa), PCP1.2 (3.5 kDa), PCP2.1 (9.1 kDa) and PCP2.2 (8.3 kDa).
32450234	0	16	theme	Pogostemon	62:71	arg1	polysaccharide					80:93	Pogostemon cablin polysaccharide	62:93	Pogostemon cablin polysaccharide	62:93	Antiviral activity against porcine epidemic diarrhea virus of Pogostemon cablin polysaccharide.
32450234	21	17	theme	antiviral	2281:2289	arg1	activities					2291:2300	their antiviral activities	2275:2300	their antiviral activities against PEDV	2275:2313	Furthermore, the anti-oxidative effects of PCPs play important roles in their antiviral activities against PEDV.
32450234	0	18	theme	polysaccharide	80:93	arg1	virus					53:57	porcine epidemic diarrhea virus	27:57	porcine epidemic diarrhea virus of Pogostemon cablin polysaccharide	27:93	Antiviral activity against porcine epidemic diarrhea virus of Pogostemon cablin polysaccharide.
32450234	7	19	theme	epidemic	602:609	arg1	PEDV					627:630	PEDV	627:630	PEDV	627:630	But it is not known whether Pogostemon cablin polysaccharides (PCPs) has the antiviral activities against porcine epidemic diarrhea virus (PEDV).
32450234	7	19	theme	epidemic	602:609	arg1	virus					620:624	porcine epidemic diarrhea virus	594:624	porcine epidemic diarrhea virus (PEDV)	594:631	But it is not known whether Pogostemon cablin polysaccharides (PCPs) has the antiviral activities against porcine epidemic diarrhea virus (PEDV).
32450234	3	20	theme	Pogostemon	350:359	arg1	Benth					377:381	Pogostemon cablin (Blanco) Benth	350:381	Pogostemon cablin (Blanco) Benth	350:381	Polysaccharide is an important component of Pogostemon cablin (Blanco) Benth.
32450234	11	21	theme	MTT	1175:1177	arg1	method					1179:1184	MTT method	1175:1184	MTT method	1175:1184	Afterwards, the antiviral effect of PCPs against PEDV on IPEC-J2 cells was studied by MTT method and real-time PCR method.
32450234	8	22	theme	PCPs	762:765	arg1	activities					748:757	the anti-PEDV activities	734:757	the anti-PEDV activities of PCPs	734:765	AIM OF THE STUDY The purpose of present study is to investigate the structural characterization and the anti-PEDV activities of PCPs.
32450234	8	22	theme	PCPs	762:765	arg1	characterization					713:728	the structural characterization	698:728	the structural characterization	698:728	AIM OF THE STUDY The purpose of present study is to investigate the structural characterization and the anti-PEDV activities of PCPs.
32450234	15	23	theme	galacturonic	1676:1687	arg1	acid					1689:1692	galacturonic acid	1676:1692	galacturonic acid	1676:1692	PCP1.1, PCP1.2 and PCP2.1 were composed of fucose, arabinose, galactose, glucose, mannose, galacturonic acid and glucuronic acid; and PCP2.2 was composed of arabinose, galactose, glucose, galacturonic acid and glucuronic acid.
32450234	7	24	theme	antiviral	565:573	arg1	activities					575:584	the antiviral activities	561:584	the antiviral activities against porcine epidemic diarrhea virus (PEDV)	561:631	But it is not known whether Pogostemon cablin polysaccharides (PCPs) has the antiviral activities against porcine epidemic diarrhea virus (PEDV).
32450234	0	25	theme	Antiviral	0:8	arg1	activity					10:17	Antiviral activity	0:17	Antiviral activity against porcine epidemic diarrhea virus of Pogostemon cablin polysaccharide	0:93	Antiviral activity against porcine epidemic diarrhea virus of Pogostemon cablin polysaccharide.
32450234	6	26	theme	epidemic	469:476	arg1	diarrhea					478:485	porcine epidemic diarrhea	461:485	porcine epidemic diarrhea	461:485	is used to treat porcine epidemic diarrhea.
32450234	15	27	theme	glucuronic	1698:1707	arg1	acid					1709:1712	glucuronic acid	1698:1712	glucuronic acid	1698:1712	PCP1.1, PCP1.2 and PCP2.1 were composed of fucose, arabinose, galactose, glucose, mannose, galacturonic acid and glucuronic acid; and PCP2.2 was composed of arabinose, galactose, glucose, galacturonic acid and glucuronic acid.
32450234	7	28	theme	cablin	527:532	arg1	polysaccharides					534:548	Pogostemon cablin polysaccharides	516:548	Pogostemon cablin polysaccharides (PCPs)	516:555	But it is not known whether Pogostemon cablin polysaccharides (PCPs) has the antiviral activities against porcine epidemic diarrhea virus (PEDV).
32450234	7	28	theme	cablin	527:532	arg1	PCPs					551:554	PCPs	551:554	PCPs	551:554	But it is not known whether Pogostemon cablin polysaccharides (PCPs) has the antiviral activities against porcine epidemic diarrhea virus (PEDV).
32450234	11	29	theme	real-time	1190:1198	arg1	PCR					1200:1202	real-time PCR	1190:1202	real-time PCR method	1190:1209	Afterwards, the antiviral effect of PCPs against PEDV on IPEC-J2 cells was studied by MTT method and real-time PCR method.
32450234	0	30	theme	epidemic	35:42	arg1	virus					53:57	porcine epidemic diarrhea virus	27:57	porcine epidemic diarrhea virus of Pogostemon cablin polysaccharide	27:93	Antiviral activity against porcine epidemic diarrhea virus of Pogostemon cablin polysaccharide.
32450234	20	31	theme	porcine	2127:2133	arg1	diarrhea					2144:2151	porcine epidemic diarrhea	2127:2151	porcine epidemic diarrhea	2127:2151	on porcine epidemic diarrhea might be related to the anti-PEDV effect of PCPs.
32450234	18	32	theme	anti-oxidative	1980:1993	arg1	effects					1995:2001	anti-oxidative effects	1980:2001	anti-oxidative effects	1980:2001	All PCPs showed anti-oxidative effects, which were important to the anti-PEDV activities.
32450234	12	33	from	effects	1230:1236	arg1	replication					1282:1292	replication	1282:1292	replication	1282:1292	Additionally, the effects of PCPs on PEDV adsorption, penetration and replication were analyzed by real-time PCR method.
32450234	12	33	from	effects	1230:1236	arg1	penetration					1266:1276	penetration	1266:1276	penetration	1266:1276	Additionally, the effects of PCPs on PEDV adsorption, penetration and replication were analyzed by real-time PCR method.
32450234	12	33	from	effects	1230:1236	arg1	adsorption					1254:1263	PEDV adsorption	1249:1263	PEDV adsorption	1249:1263	Additionally, the effects of PCPs on PEDV adsorption, penetration and replication were analyzed by real-time PCR method.
32450234	9	34	theme	precipitation	841:853	arg1	method					855:860	alcohol precipitation method	833:860	alcohol precipitation method	833:860	MATERIALS AND METHODS PCPs were prepared by water extraction and alcohol precipitation method and purified with DEAE-52 cellulose column and Sephadex G-100 column.
32450234	11	35	theme	PCR	1200:1202	arg1	method					1204:1209	real-time PCR method	1190:1209	real-time PCR method	1190:1209	Afterwards, the antiviral effect of PCPs against PEDV on IPEC-J2 cells was studied by MTT method and real-time PCR method.
32450234	16	36	theme	anti-PEDV	1828:1836	arg1	activities					1838:1847	anti-PEDV activities	1828:1847	anti-PEDV activities	1828:1847	All PCPs showed anti-PEDV activities.
32450234	17	37	theme	PEDV	1878:1881	arg1	replication					1883:1893	PEDV replication	1878:1893	PEDV replication	1878:1893	PCP1.1 and PCP1.2 inhibited PEDV replication, while PCP2.1 and PCP2.2 inhibited PEDV penetration and replication.
32450234	10	38	theme	structural	942:951	arg1	characterization					953:968	the structural characterization	938:968	the structural characterization of the polysaccharides including the infrared spectrum, molecular weight and monosaccharide composition	938:1072	Then, the structural characterization of the polysaccharides including the infrared spectrum, molecular weight and monosaccharide composition were analyzed.
32450234	9	39	theme	cellulose	888:896	arg1	column					898:903	DEAE-52 cellulose column	880:903	DEAE-52 cellulose column	880:903	MATERIALS AND METHODS PCPs were prepared by water extraction and alcohol precipitation method and purified with DEAE-52 cellulose column and Sephadex G-100 column.
32450234	13	40	theme	PCPs	1404:1407	arg1	effects					1393:1399	the anti-oxidative effects	1374:1399	the anti-oxidative effects of PCPs	1374:1407	Furthermore, we also investigate whether the anti-oxidative effects of PCPs were important to the anti-PEDV activities.
32450234	13	40	theme	PCPs	1404:1407	arg1	important					1414:1422	important	1414:1422	important	1414:1422	Furthermore, we also investigate whether the anti-oxidative effects of PCPs were important to the anti-PEDV activities.
32450234	20	41	theme	anti-PEDV	2177:2185	arg1	effect					2187:2192	the anti-PEDV effect	2173:2192	the anti-PEDV effect of PCPs	2173:2200	on porcine epidemic diarrhea might be related to the anti-PEDV effect of PCPs.
32450234	9	42	theme	G-100	918:922	arg1	column					924:929	Sephadex G-100 column	909:929	Sephadex G-100 column	909:929	MATERIALS AND METHODS PCPs were prepared by water extraction and alcohol precipitation method and purified with DEAE-52 cellulose column and Sephadex G-100 column.
32450234	19	43	theme	treatment	2070:2078	arg1	effect					2080:2085	The treatment effect	2066:2085	The treatment effect of Pogostemon cablin (Blanco) Benth	2066:2121	CONCLUSIONS The treatment effect of Pogostemon cablin (Blanco) Benth.
32450234	1	44	theme	Benth	182:186	arg1	parts					146:150	overground parts	135:150	overground parts of Pogostemon cablin (Blanco) Benth	135:186	ETHNOPHARMACOLOGICAL RELEVANCE The dry overground parts of Pogostemon cablin (Blanco) Benth.
32450234	8	45	theme	STUDY	645:649	arg1	AIM					634:636	AIM	634:636	AIM OF THE STUDY The purpose of present study is to investigate the structural characterization and the anti-PEDV activities of PCPs.	634:766	AIM OF THE STUDY The purpose of present study is to investigate the structural characterization and the anti-PEDV activities of PCPs.
32450234	10	46	theme	monosaccharide	1047:1060	arg1	composition					1062:1072	monosaccharide composition	1047:1072	monosaccharide composition	1047:1072	Then, the structural characterization of the polysaccharides including the infrared spectrum, molecular weight and monosaccharide composition were analyzed.
32450234	15	47	theme	glucuronic	1795:1804	arg1	acid					1806:1809	glucuronic acid	1795:1809	glucuronic acid	1795:1809	PCP1.1, PCP1.2 and PCP2.1 were composed of fucose, arabinose, galactose, glucose, mannose, galacturonic acid and glucuronic acid; and PCP2.2 was composed of arabinose, galactose, glucose, galacturonic acid and glucuronic acid.
32450234	15	47	theme	glucuronic	1795:1804	arg1	arabinose					1742:1750	arabinose	1742:1750	arabinose	1742:1750	PCP1.1, PCP1.2 and PCP2.1 were composed of fucose, arabinose, galactose, glucose, mannose, galacturonic acid and glucuronic acid; and PCP2.2 was composed of arabinose, galactose, glucose, galacturonic acid and glucuronic acid.
32450234	19	48	theme	cablin	2101:2106	arg1	Benth					2117:2121	Pogostemon cablin (Blanco) Benth	2090:2121	Pogostemon cablin (Blanco) Benth	2090:2121	CONCLUSIONS The treatment effect of Pogostemon cablin (Blanco) Benth.
32450234	11	49	theme	antiviral	1105:1113	arg1	effect					1115:1120	the antiviral effect	1101:1120	the antiviral effect of PCPs against PEDV on IPEC-J2 cells	1101:1158	Afterwards, the antiviral effect of PCPs against PEDV on IPEC-J2 cells was studied by MTT method and real-time PCR method.
32450234	21	50	theme	anti-oxidative	2220:2233	arg1	effects					2235:2241	the anti-oxidative effects	2216:2241	the anti-oxidative effects of PCPs	2216:2249	Furthermore, the anti-oxidative effects of PCPs play important roles in their antiviral activities against PEDV.
32450234	13	51	theme	anti-oxidative	1378:1391	arg1	effects					1393:1399	the anti-oxidative effects	1374:1399	the anti-oxidative effects of PCPs	1374:1407	Furthermore, we also investigate whether the anti-oxidative effects of PCPs were important to the anti-PEDV activities.
32450234	13	51	theme	anti-oxidative	1378:1391	arg1	important					1414:1422	important	1414:1422	important	1414:1422	Furthermore, we also investigate whether the anti-oxidative effects of PCPs were important to the anti-PEDV activities.
32450234	12	52	theme	real-time	1311:1319	arg1	PCR					1321:1323	real-time PCR	1311:1323	real-time PCR method	1311:1330	Additionally, the effects of PCPs on PEDV adsorption, penetration and replication were analyzed by real-time PCR method.
32450234	19	53	theme	Benth	2117:2121	arg1	effect					2080:2085	The treatment effect	2066:2085	The treatment effect of Pogostemon cablin (Blanco) Benth	2066:2121	CONCLUSIONS The treatment effect of Pogostemon cablin (Blanco) Benth.
32450234	10	54	theme	polysaccharides	977:991	arg1	characterization					953:968	the structural characterization	938:968	the structural characterization of the polysaccharides including the infrared spectrum, molecular weight and monosaccharide composition	938:1072	Then, the structural characterization of the polysaccharides including the infrared spectrum, molecular weight and monosaccharide composition were analyzed.
32450234	2	55	theme	traditional	218:228	arg1	medicine					238:245	a traditional Chinese medicine	216:245	a traditional Chinese medicine for the treatment of diarrhea, vomiting, nausea and fever	216:303	is widely used in China as a traditional Chinese medicine for the treatment of diarrhea, vomiting, nausea and fever.
32450234	1	56	theme	overground	135:144	arg1	parts					146:150	overground parts	135:150	overground parts of Pogostemon cablin (Blanco) Benth	135:186	ETHNOPHARMACOLOGICAL RELEVANCE The dry overground parts of Pogostemon cablin (Blanco) Benth.
32450234	17	57	theme	PEDV	1930:1933	arg1	penetration					1935:1945	PEDV penetration	1930:1945	PEDV penetration	1930:1945	PCP1.1 and PCP1.2 inhibited PEDV replication, while PCP2.1 and PCP2.2 inhibited PEDV penetration and replication.
32450234	19	58	theme	Blanco	2109:2114	arg1	Benth					2117:2121	Pogostemon cablin (Blanco) Benth	2090:2121	Pogostemon cablin (Blanco) Benth	2090:2121	CONCLUSIONS The treatment effect of Pogostemon cablin (Blanco) Benth.
32450234	10	59	theme	molecular	1026:1034	arg1	weight					1036:1041	molecular weight	1026:1041	molecular weight	1026:1041	Then, the structural characterization of the polysaccharides including the infrared spectrum, molecular weight and monosaccharide composition were analyzed.
32450234	8	60	theme	structural	702:711	arg1	characterization					713:728	the structural characterization	698:728	the structural characterization	698:728	AIM OF THE STUDY The purpose of present study is to investigate the structural characterization and the anti-PEDV activities of PCPs.
32450234	0	61	theme	cablin	73:78	arg1	polysaccharide					80:93	Pogostemon cablin polysaccharide	62:93	Pogostemon cablin polysaccharide	62:93	Antiviral activity against porcine epidemic diarrhea virus of Pogostemon cablin polysaccharide.
32450234	18	62	theme	anti-PEDV	2032:2040	arg1	activities					2042:2051	the anti-PEDV activities	2028:2051	the anti-PEDV activities	2028:2051	All PCPs showed anti-oxidative effects, which were important to the anti-PEDV activities.
32450234	3	63	theme	important	327:335	arg1	Polysaccharide					306:319	Polysaccharide	306:319	Polysaccharide	306:319	Polysaccharide is an important component of Pogostemon cablin (Blanco) Benth.
32450234	3	63	theme	important	327:335	arg1	component					337:345	an important component	324:345	an important component of Pogostemon cablin (Blanco) Benth	324:381	Polysaccharide is an important component of Pogostemon cablin (Blanco) Benth.
32450234	8	64	dep	AIM	634:636	arg1	is					680:681	is	680:681	is to investigate the structural characterization and the anti-PEDV activities of PCPs	680:765	AIM OF THE STUDY The purpose of present study is to investigate the structural characterization and the anti-PEDV activities of PCPs.
32450234	9	65	theme	MATERIALS	768:776	arg1	PCPs					790:793	MATERIALS AND METHODS PCPs	768:793	MATERIALS AND METHODS PCPs	768:793	MATERIALS AND METHODS PCPs were prepared by water extraction and alcohol precipitation method and purified with DEAE-52 cellulose column and Sephadex G-100 column.
32450234	1	66	dep	RELEVANCE	117:125	arg1	The					127:129	The	127:129	The	127:129	ETHNOPHARMACOLOGICAL RELEVANCE The dry overground parts of Pogostemon cablin (Blanco) Benth.
32450234	8	67	theme	anti-PEDV	738:746	arg1	activities					748:757	the anti-PEDV activities	734:757	the anti-PEDV activities of PCPs	734:765	AIM OF THE STUDY The purpose of present study is to investigate the structural characterization and the anti-PEDV activities of PCPs.
32450234	7	68	theme	diarrhea	611:618	arg1	PEDV					627:630	PEDV	627:630	PEDV	627:630	But it is not known whether Pogostemon cablin polysaccharides (PCPs) has the antiviral activities against porcine epidemic diarrhea virus (PEDV).
32450234	7	68	theme	diarrhea	611:618	arg1	virus					620:624	porcine epidemic diarrhea virus	594:624	porcine epidemic diarrhea virus (PEDV)	594:631	But it is not known whether Pogostemon cablin polysaccharides (PCPs) has the antiviral activities against porcine epidemic diarrhea virus (PEDV).
32450234	3	69	theme	cablin	361:366	arg1	Benth					377:381	Pogostemon cablin (Blanco) Benth	350:381	Pogostemon cablin (Blanco) Benth	350:381	Polysaccharide is an important component of Pogostemon cablin (Blanco) Benth.
32450234	9	70	theme	METHODS	782:788	arg1	PCPs					790:793	MATERIALS AND METHODS PCPs	768:793	MATERIALS AND METHODS PCPs	768:793	MATERIALS AND METHODS PCPs were prepared by water extraction and alcohol precipitation method and purified with DEAE-52 cellulose column and Sephadex G-100 column.
32450234	7	71	theme	porcine	594:600	arg1	PEDV					627:630	PEDV	627:630	PEDV	627:630	But it is not known whether Pogostemon cablin polysaccharides (PCPs) has the antiviral activities against porcine epidemic diarrhea virus (PEDV).
32450234	7	71	theme	porcine	594:600	arg1	virus					620:624	porcine epidemic diarrhea virus	594:624	porcine epidemic diarrhea virus (PEDV)	594:631	But it is not known whether Pogostemon cablin polysaccharides (PCPs) has the antiviral activities against porcine epidemic diarrhea virus (PEDV).
32450234	11	72	theme	IPEC-J2	1146:1152	arg1	cells					1154:1158	IPEC-J2 cells	1146:1158	IPEC-J2 cells	1146:1158	Afterwards, the antiviral effect of PCPs against PEDV on IPEC-J2 cells was studied by MTT method and real-time PCR method.
32450234	8	73	theme	present	666:672	arg1	study					674:678	present study	666:678	present study	666:678	AIM OF THE STUDY The purpose of present study is to investigate the structural characterization and the anti-PEDV activities of PCPs.
32450234	1	74	theme	ETHNOPHARMACOLOGICAL	96:115	arg1	RELEVANCE					117:125	ETHNOPHARMACOLOGICAL RELEVANCE	96:125	ETHNOPHARMACOLOGICAL RELEVANCE The	96:129	ETHNOPHARMACOLOGICAL RELEVANCE The dry overground parts of Pogostemon cablin (Blanco) Benth.
32450234	3	75	theme	Benth	377:381	arg1	Polysaccharide					306:319	Polysaccharide	306:319	Polysaccharide	306:319	Polysaccharide is an important component of Pogostemon cablin (Blanco) Benth.
32450234	3	75	theme	Benth	377:381	arg1	component					337:345	an important component	324:345	an important component of Pogostemon cablin (Blanco) Benth	324:381	Polysaccharide is an important component of Pogostemon cablin (Blanco) Benth.
32450234	0	76	theme	porcine	27:33	arg1	virus					53:57	porcine epidemic diarrhea virus	27:57	porcine epidemic diarrhea virus of Pogostemon cablin polysaccharide	27:93	Antiviral activity against porcine epidemic diarrhea virus of Pogostemon cablin polysaccharide.
32450234	7	77	theme	Pogostemon	516:525	arg1	polysaccharides					534:548	Pogostemon cablin polysaccharides	516:548	Pogostemon cablin polysaccharides (PCPs)	516:555	But it is not known whether Pogostemon cablin polysaccharides (PCPs) has the antiviral activities against porcine epidemic diarrhea virus (PEDV).
32450234	7	77	theme	Pogostemon	516:525	arg1	PCPs					551:554	PCPs	551:554	PCPs	551:554	But it is not known whether Pogostemon cablin polysaccharides (PCPs) has the antiviral activities against porcine epidemic diarrhea virus (PEDV).
32450234	12	78	theme	PCPs	1241:1244	arg1	effects					1230:1236	the effects	1226:1236	the effects of PCPs on PEDV adsorption, penetration and replication	1226:1292	Additionally, the effects of PCPs on PEDV adsorption, penetration and replication were analyzed by real-time PCR method.
32450234	3	79	theme	Blanco	369:374	arg1	Benth					377:381	Pogostemon cablin (Blanco) Benth	350:381	Pogostemon cablin (Blanco) Benth	350:381	Polysaccharide is an important component of Pogostemon cablin (Blanco) Benth.
32450234	6	80	theme	porcine	461:467	arg1	diarrhea					478:485	porcine epidemic diarrhea	461:485	porcine epidemic diarrhea	461:485	is used to treat porcine epidemic diarrhea.
32450234	11	81	from	effect	1115:1120	arg1	cells					1154:1158	IPEC-J2 cells	1146:1158	IPEC-J2 cells	1146:1158	Afterwards, the antiviral effect of PCPs against PEDV on IPEC-J2 cells was studied by MTT method and real-time PCR method.
32450234	0	82	theme	diarrhea	44:51	arg1	virus					53:57	porcine epidemic diarrhea virus	27:57	porcine epidemic diarrhea virus of Pogostemon cablin polysaccharide	27:93	Antiviral activity against porcine epidemic diarrhea virus of Pogostemon cablin polysaccharide.
32450234	12	83	theme	PEDV	1249:1252	arg1	adsorption					1254:1263	PEDV adsorption	1249:1263	PEDV adsorption	1249:1263	Additionally, the effects of PCPs on PEDV adsorption, penetration and replication were analyzed by real-time PCR method.
32450234	9	84	theme	alcohol	833:839	arg1	method					855:860	alcohol precipitation method	833:860	alcohol precipitation method	833:860	MATERIALS AND METHODS PCPs were prepared by water extraction and alcohol precipitation method and purified with DEAE-52 cellulose column and Sephadex G-100 column.
32450234	20	85	theme	epidemic	2135:2142	arg1	diarrhea					2144:2151	porcine epidemic diarrhea	2127:2151	porcine epidemic diarrhea	2127:2151	on porcine epidemic diarrhea might be related to the anti-PEDV effect of PCPs.
32450234	13	86	theme	anti-PEDV	1431:1439	arg1	activities					1441:1450	the anti-PEDV activities	1427:1450	the anti-PEDV activities	1427:1450	Furthermore, we also investigate whether the anti-oxidative effects of PCPs were important to the anti-PEDV activities.
32450234	20	87	theme	PCPs	2197:2200	arg1	effect					2187:2192	the anti-PEDV effect	2173:2192	the anti-PEDV effect of PCPs	2173:2200	on porcine epidemic diarrhea might be related to the anti-PEDV effect of PCPs.
32450234	11	88	theme	PCPs	1125:1128	arg1	effect					1115:1120	the antiviral effect	1101:1120	the antiviral effect of PCPs against PEDV on IPEC-J2 cells	1101:1158	Afterwards, the antiviral effect of PCPs against PEDV on IPEC-J2 cells was studied by MTT method and real-time PCR method.
32450234	9	89	theme	DEAE-52	880:886	arg1	column					898:903	DEAE-52 cellulose column	880:903	DEAE-52 cellulose column	880:903	MATERIALS AND METHODS PCPs were prepared by water extraction and alcohol precipitation method and purified with DEAE-52 cellulose column and Sephadex G-100 column.
34492480	6	0	theme	@	1036:1036	arg1	HG					1037:1038	The NF@HG	1030:1038	The NF@HG	1030:1038	The NF@HG exhibited enhanced antibacterial ability and the sustained release of TH can provide long-term antibacterial activity.
34492480	1	1	theme	stretchable	192:202	arg1	transparency					151:162	transparency	151:162	transparency	151:162	Multifunctional hydrogels with transparency, ultraviolet (UV)-blocking, stretchable, self-healing, adhesive, antioxidant and antibacterial properties are promising materials for biomedical and relevant applications.
34492480	1	2	theme	biomedical	298:307	arg1	applications					322:333	biomedical and relevant applications	298:333	biomedical and relevant applications	298:333	Multifunctional hydrogels with transparency, ultraviolet (UV)-blocking, stretchable, self-healing, adhesive, antioxidant and antibacterial properties are promising materials for biomedical and relevant applications.
34492480	8	3	from	aspects	1323:1329	arg1	preparation					1354:1364	fast and economical preparation	1334:1364	fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials	1334:1479	Taken together, this strategy based on self-catalytic Fe3+/TA-CNF system may inspire new aspects on fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials.
34492480	3	4	theme	acid-cellulose	589:602	arg1	Fe3+/TA-CNF					615:625	Fe3+/TA-CNF	615:625	Fe3+/TA-CNF	615:625	Here, a series of hydrogels possessing ultrashort gelation time (~30 s) at room or cold temperature were fabricated based on self-catalytic Fe3+/Tannic acid-cellulose nanofiber (Fe3+/TA-CNF).
34492480	3	4	theme	acid-cellulose	589:602	arg1	nanofiber					604:612	self-catalytic Fe3+/Tannic acid-cellulose nanofiber	562:612	self-catalytic Fe3+/Tannic acid-cellulose nanofiber (Fe3+/TA-CNF)	562:626	Here, a series of hydrogels possessing ultrashort gelation time (~30 s) at room or cold temperature were fabricated based on self-catalytic Fe3+/Tannic acid-cellulose nanofiber (Fe3+/TA-CNF).
34492480	4	5	theme	redox	655:659	arg1	pairs					661:665	stable redox pairs	648:665	stable redox pairs	648:665	Fe3+/TA-CNF formed stable redox pairs to activate ammonium persulfate (initiator), generating abundant free radicals to trigger the ultrafast polymerization of acrylic acid (AA).
34492480	2	6	theme	hydrogels	366:374	arg1	challenge					426:434	a challenge	424:434	a challenge	424:434	However, preparation of these hydrogels at ambient environment without stimuli is still a challenge.
34492480	2	6	theme	hydrogels	366:374	arg1	preparation					345:355	preparation	345:355	preparation of these hydrogels at ambient environment without stimuli	345:413	However, preparation of these hydrogels at ambient environment without stimuli is still a challenge.
34492480	8	7	theme	multifunctional	1369:1383	arg1	hydrogels					1385:1393	multifunctional hydrogels	1369:1393	multifunctional hydrogels	1369:1393	Taken together, this strategy based on self-catalytic Fe3+/TA-CNF system may inspire new aspects on fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials.
34492480	7	8	theme	viability	1173:1181	arg1	results					1183:1189	cell viability results	1168:1189	cell viability results	1168:1189	Besides, cell viability results demonstrated that NF@HG was non-cytotoxic.
34492480	4	9	theme	acrylic	789:795	arg1	AA					803:804	AA	803:804	AA	803:804	Fe3+/TA-CNF formed stable redox pairs to activate ammonium persulfate (initiator), generating abundant free radicals to trigger the ultrafast polymerization of acrylic acid (AA).
34492480	4	9	theme	acrylic	789:795	arg1	acid					797:800	acrylic acid	789:800	acrylic acid (AA)	789:805	Fe3+/TA-CNF formed stable redox pairs to activate ammonium persulfate (initiator), generating abundant free radicals to trigger the ultrafast polymerization of acrylic acid (AA).
34492480	1	10	theme	relevant	313:320	arg1	applications					322:333	biomedical and relevant applications	298:333	biomedical and relevant applications	298:333	Multifunctional hydrogels with transparency, ultraviolet (UV)-blocking, stretchable, self-healing, adhesive, antioxidant and antibacterial properties are promising materials for biomedical and relevant applications.
34492480	2	11	from	environment	387:397	arg1	challenge					426:434	a challenge	424:434	a challenge	424:434	However, preparation of these hydrogels at ambient environment without stimuli is still a challenge.
34492480	2	11	from	environment	387:397	arg1	preparation					345:355	preparation	345:355	preparation of these hydrogels at ambient environment without stimuli	345:413	However, preparation of these hydrogels at ambient environment without stimuli is still a challenge.
34492480	3	12	theme	self-catalytic	562:575	arg1	Fe3+/TA-CNF					615:625	Fe3+/TA-CNF	615:625	Fe3+/TA-CNF	615:625	Here, a series of hydrogels possessing ultrashort gelation time (~30 s) at room or cold temperature were fabricated based on self-catalytic Fe3+/Tannic acid-cellulose nanofiber (Fe3+/TA-CNF).
34492480	3	12	theme	self-catalytic	562:575	arg1	nanofiber					604:612	self-catalytic Fe3+/Tannic acid-cellulose nanofiber	562:612	self-catalytic Fe3+/Tannic acid-cellulose nanofiber (Fe3+/TA-CNF)	562:626	Here, a series of hydrogels possessing ultrashort gelation time (~30 s) at room or cold temperature were fabricated based on self-catalytic Fe3+/Tannic acid-cellulose nanofiber (Fe3+/TA-CNF).
34492480	5	13	theme	electrospun	946:956	arg1	nanofibers					958:967	tetracycline hydrochloride (TH)-loaded electrospun nanofibers	907:967	tetracycline hydrochloride (TH)-loaded electrospun nanofibers	907:967	To improve the antibacterial ability of hydrogel, a bilayer hydrogel composite (NF@HG) composed of tetracycline hydrochloride (TH)-loaded electrospun nanofibers and hydrogel layer was fabricated via a mild casting method.
34492480	3	14	theme	gelation	487:494	arg1	~30 s					502:506	~30 s	502:506	~30 s	502:506	Here, a series of hydrogels possessing ultrashort gelation time (~30 s) at room or cold temperature were fabricated based on self-catalytic Fe3+/Tannic acid-cellulose nanofiber (Fe3+/TA-CNF).
34492480	3	14	theme	gelation	487:494	arg1	time					496:499	ultrashort gelation time	476:499	ultrashort gelation time (~30 s)	476:507	Here, a series of hydrogels possessing ultrashort gelation time (~30 s) at room or cold temperature were fabricated based on self-catalytic Fe3+/Tannic acid-cellulose nanofiber (Fe3+/TA-CNF).
34492480	4	15	theme	stable	648:653	arg1	pairs					661:665	stable redox pairs	648:665	stable redox pairs	648:665	Fe3+/TA-CNF formed stable redox pairs to activate ammonium persulfate (initiator), generating abundant free radicals to trigger the ultrafast polymerization of acrylic acid (AA).
34492480	4	16	theme	abundant	723:730	arg1	radicals					737:744	abundant free radicals	723:744	abundant free radicals	723:744	Fe3+/TA-CNF formed stable redox pairs to activate ammonium persulfate (initiator), generating abundant free radicals to trigger the ultrafast polymerization of acrylic acid (AA).
34492480	3	17	theme	Fe3+/Tannic	577:587	arg1	Fe3+/TA-CNF					615:625	Fe3+/TA-CNF	615:625	Fe3+/TA-CNF	615:625	Here, a series of hydrogels possessing ultrashort gelation time (~30 s) at room or cold temperature were fabricated based on self-catalytic Fe3+/Tannic acid-cellulose nanofiber (Fe3+/TA-CNF).
34492480	3	17	theme	Fe3+/Tannic	577:587	arg1	nanofiber					604:612	self-catalytic Fe3+/Tannic acid-cellulose nanofiber	562:612	self-catalytic Fe3+/Tannic acid-cellulose nanofiber (Fe3+/TA-CNF)	562:626	Here, a series of hydrogels possessing ultrashort gelation time (~30 s) at room or cold temperature were fabricated based on self-catalytic Fe3+/Tannic acid-cellulose nanofiber (Fe3+/TA-CNF).
34492480	6	18	theme	TH	1110:1111	arg1	release					1099:1105	the sustained release	1085:1105	the sustained release of TH	1085:1111	The NF@HG exhibited enhanced antibacterial ability and the sustained release of TH can provide long-term antibacterial activity.
34492480	1	19	theme	self-healing	205:216	arg1	transparency					151:162	transparency	151:162	transparency	151:162	Multifunctional hydrogels with transparency, ultraviolet (UV)-blocking, stretchable, self-healing, adhesive, antioxidant and antibacterial properties are promising materials for biomedical and relevant applications.
34492480	7	20	theme	NF	1209:1210	arg1	HG					1212:1213	NF@HG	1209:1213	NF@HG	1209:1213	Besides, cell viability results demonstrated that NF@HG was non-cytotoxic.
34492480	8	21	contain	have	1416:1419	arg1	composites					1398:1407	composites	1398:1407	composites	1398:1407	Taken together, this strategy based on self-catalytic Fe3+/TA-CNF system may inspire new aspects on fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials.
34492480	8	21	contain	have	1416:1419	arg1	hydrogels					1385:1393	multifunctional hydrogels	1369:1393	multifunctional hydrogels	1369:1393	Taken together, this strategy based on self-catalytic Fe3+/TA-CNF system may inspire new aspects on fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials.
34492480	8	21	contain	have	1416:1419	arg2	applications					1443:1454	attractive industrial applications	1421:1454	attractive industrial applications for biomedical materials	1421:1479	Taken together, this strategy based on self-catalytic Fe3+/TA-CNF system may inspire new aspects on fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials.
34492480	1	22	theme	Multifunctional	120:134	arg1	hydrogels					136:144	Multifunctional hydrogels	120:144	Multifunctional hydrogels with transparency, ultraviolet (UV)-blocking, stretchable, self-healing, adhesive, antioxidant and antibacterial properties	120:268	Multifunctional hydrogels with transparency, ultraviolet (UV)-blocking, stretchable, self-healing, adhesive, antioxidant and antibacterial properties are promising materials for biomedical and relevant applications.
34492480	1	22	theme	Multifunctional	120:134	arg1	materials					284:292	promising materials	274:292	promising materials for biomedical and relevant applications	274:333	Multifunctional hydrogels with transparency, ultraviolet (UV)-blocking, stretchable, self-healing, adhesive, antioxidant and antibacterial properties are promising materials for biomedical and relevant applications.
34492480	6	23	theme	sustained	1089:1097	arg1	release					1099:1105	the sustained release	1085:1105	the sustained release of TH	1085:1111	The NF@HG exhibited enhanced antibacterial ability and the sustained release of TH can provide long-term antibacterial activity.
34492480	4	24	theme	free	732:735	arg1	radicals					737:744	abundant free radicals	723:744	abundant free radicals	723:744	Fe3+/TA-CNF formed stable redox pairs to activate ammonium persulfate (initiator), generating abundant free radicals to trigger the ultrafast polymerization of acrylic acid (AA).
34492480	0	25	theme	Ultrafast	0:8	arg1	gelation					10:17	Ultrafast gelation	0:17	Ultrafast gelation of multifunctional hydrogel/composite	0:55	Ultrafast gelation of multifunctional hydrogel/composite based on self-catalytic Fe3+/Tannic acid-cellulose nanofibers.
34492480	4	26	theme	acid	797:800	arg1	polymerization					771:784	the ultrafast polymerization	757:784	the ultrafast polymerization of acrylic acid (AA)	757:805	Fe3+/TA-CNF formed stable redox pairs to activate ammonium persulfate (initiator), generating abundant free radicals to trigger the ultrafast polymerization of acrylic acid (AA).
34492480	8	27	theme	Fe3+/TA-CNF	1288:1298	arg1	system					1300:1305	self-catalytic Fe3+/TA-CNF system	1273:1305	self-catalytic Fe3+/TA-CNF system	1273:1305	Taken together, this strategy based on self-catalytic Fe3+/TA-CNF system may inspire new aspects on fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials.
34492480	1	28	theme	adhesive	219:226	arg1	transparency					151:162	transparency	151:162	transparency	151:162	Multifunctional hydrogels with transparency, ultraviolet (UV)-blocking, stretchable, self-healing, adhesive, antioxidant and antibacterial properties are promising materials for biomedical and relevant applications.
34492480	0	29	theme	multifunctional	22:36	arg1	hydrogel/composite					38:55	multifunctional hydrogel/composite	22:55	multifunctional hydrogel/composite	22:55	Ultrafast gelation of multifunctional hydrogel/composite based on self-catalytic Fe3+/Tannic acid-cellulose nanofibers.
34492480	8	30	theme	fast	1334:1337	arg1	preparation					1354:1364	fast and economical preparation	1334:1364	fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials	1334:1479	Taken together, this strategy based on self-catalytic Fe3+/TA-CNF system may inspire new aspects on fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials.
34492480	8	31	theme	attractive	1421:1430	arg1	applications					1443:1454	attractive industrial applications	1421:1454	attractive industrial applications for biomedical materials	1421:1479	Taken together, this strategy based on self-catalytic Fe3+/TA-CNF system may inspire new aspects on fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials.
34492480	1	32	with	hydrogels	136:144	arg1	properties					259:268	transparency, ultraviolet (UV)-blocking, stretchable, self-healing, adhesive, antioxidant and antibacterial properties	151:268	transparency, ultraviolet (UV)-blocking, stretchable, self-healing, adhesive, antioxidant and antibacterial properties	151:268	Multifunctional hydrogels with transparency, ultraviolet (UV)-blocking, stretchable, self-healing, adhesive, antioxidant and antibacterial properties are promising materials for biomedical and relevant applications.
34492480	5	33	theme	hydrogel	973:980	arg1	layer					982:986	hydrogel layer	973:986	hydrogel layer	973:986	To improve the antibacterial ability of hydrogel, a bilayer hydrogel composite (NF@HG) composed of tetracycline hydrochloride (TH)-loaded electrospun nanofibers and hydrogel layer was fabricated via a mild casting method.
34492480	8	34	theme	industrial	1432:1441	arg1	applications					1443:1454	attractive industrial applications	1421:1454	attractive industrial applications for biomedical materials	1421:1479	Taken together, this strategy based on self-catalytic Fe3+/TA-CNF system may inspire new aspects on fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials.
34492480	1	35	theme	transparency	151:162	arg1	properties					259:268	transparency, ultraviolet (UV)-blocking, stretchable, self-healing, adhesive, antioxidant and antibacterial properties	151:268	transparency, ultraviolet (UV)-blocking, stretchable, self-healing, adhesive, antioxidant and antibacterial properties	151:268	Multifunctional hydrogels with transparency, ultraviolet (UV)-blocking, stretchable, self-healing, adhesive, antioxidant and antibacterial properties are promising materials for biomedical and relevant applications.
34492480	1	36	theme	antioxidant	229:239	arg1	transparency					151:162	transparency	151:162	transparency	151:162	Multifunctional hydrogels with transparency, ultraviolet (UV)-blocking, stretchable, self-healing, adhesive, antioxidant and antibacterial properties are promising materials for biomedical and relevant applications.
34492480	5	37	theme	antibacterial	823:835	arg1	ability					837:843	the antibacterial ability	819:843	the antibacterial ability of hydrogel	819:855	To improve the antibacterial ability of hydrogel, a bilayer hydrogel composite (NF@HG) composed of tetracycline hydrochloride (TH)-loaded electrospun nanofibers and hydrogel layer was fabricated via a mild casting method.
34492480	5	38	theme	-loaded	938:944	arg1	nanofibers					958:967	tetracycline hydrochloride (TH)-loaded electrospun nanofibers	907:967	tetracycline hydrochloride (TH)-loaded electrospun nanofibers	907:967	To improve the antibacterial ability of hydrogel, a bilayer hydrogel composite (NF@HG) composed of tetracycline hydrochloride (TH)-loaded electrospun nanofibers and hydrogel layer was fabricated via a mild casting method.
34492480	6	39	theme	antibacterial	1059:1071	arg1	ability					1073:1079	enhanced antibacterial ability	1050:1079	enhanced antibacterial ability	1050:1079	The NF@HG exhibited enhanced antibacterial ability and the sustained release of TH can provide long-term antibacterial activity.
34492480	8	40	theme	economical	1343:1352	arg1	preparation					1354:1364	fast and economical preparation	1334:1364	fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials	1334:1479	Taken together, this strategy based on self-catalytic Fe3+/TA-CNF system may inspire new aspects on fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials.
34492480	3	41	theme	ultrashort	476:485	arg1	~30 s					502:506	~30 s	502:506	~30 s	502:506	Here, a series of hydrogels possessing ultrashort gelation time (~30 s) at room or cold temperature were fabricated based on self-catalytic Fe3+/Tannic acid-cellulose nanofiber (Fe3+/TA-CNF).
34492480	3	41	theme	ultrashort	476:485	arg1	time					496:499	ultrashort gelation time	476:499	ultrashort gelation time (~30 s)	476:507	Here, a series of hydrogels possessing ultrashort gelation time (~30 s) at room or cold temperature were fabricated based on self-catalytic Fe3+/Tannic acid-cellulose nanofiber (Fe3+/TA-CNF).
34492480	5	42	theme	NF	888:889	arg1	composite					877:885	a bilayer hydrogel composite	858:885	a bilayer hydrogel composite (NF@HG) composed of tetracycline hydrochloride (TH)-loaded electrospun nanofibers	858:967	To improve the antibacterial ability of hydrogel, a bilayer hydrogel composite (NF@HG) composed of tetracycline hydrochloride (TH)-loaded electrospun nanofibers and hydrogel layer was fabricated via a mild casting method.
34492480	5	42	theme	NF	888:889	arg1	HG					891:892	NF@HG	888:892	NF@HG	888:892	To improve the antibacterial ability of hydrogel, a bilayer hydrogel composite (NF@HG) composed of tetracycline hydrochloride (TH)-loaded electrospun nanofibers and hydrogel layer was fabricated via a mild casting method.
34492480	0	43	theme	hydrogel/composite	38:55	arg1	gelation					10:17	Ultrafast gelation	0:17	Ultrafast gelation of multifunctional hydrogel/composite	0:55	Ultrafast gelation of multifunctional hydrogel/composite based on self-catalytic Fe3+/Tannic acid-cellulose nanofibers.
34492480	8	44	theme	new	1319:1321	arg1	aspects					1323:1329	new aspects	1319:1329	new aspects on fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials	1319:1479	Taken together, this strategy based on self-catalytic Fe3+/TA-CNF system may inspire new aspects on fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials.
34492480	3	45	theme	hydrogels	455:463	arg1	series					445:450	a series	443:450	a series of hydrogels possessing ultrashort gelation time (~30 s) at room or cold temperature	443:535	Here, a series of hydrogels possessing ultrashort gelation time (~30 s) at room or cold temperature were fabricated based on self-catalytic Fe3+/Tannic acid-cellulose nanofiber (Fe3+/TA-CNF).
34492480	4	46	theme	ammonium	679:686	arg1	initiator					700:708	initiator	700:708	initiator	700:708	Fe3+/TA-CNF formed stable redox pairs to activate ammonium persulfate (initiator), generating abundant free radicals to trigger the ultrafast polymerization of acrylic acid (AA).
34492480	4	46	theme	ammonium	679:686	arg1	persulfate					688:697	ammonium persulfate	679:697	ammonium persulfate (initiator)	679:709	Fe3+/TA-CNF formed stable redox pairs to activate ammonium persulfate (initiator), generating abundant free radicals to trigger the ultrafast polymerization of acrylic acid (AA).
34492480	1	47	theme	antibacterial	245:257	arg1	transparency					151:162	transparency	151:162	transparency	151:162	Multifunctional hydrogels with transparency, ultraviolet (UV)-blocking, stretchable, self-healing, adhesive, antioxidant and antibacterial properties are promising materials for biomedical and relevant applications.
34492480	0	48	theme	self-catalytic	66:79	arg1	nanofibers					108:117	self-catalytic Fe3+/Tannic acid-cellulose nanofibers	66:117	self-catalytic Fe3+/Tannic acid-cellulose nanofibers	66:117	Ultrafast gelation of multifunctional hydrogel/composite based on self-catalytic Fe3+/Tannic acid-cellulose nanofibers.
34492480	6	49	theme	enhanced	1050:1057	arg1	ability					1073:1079	enhanced antibacterial ability	1050:1079	enhanced antibacterial ability	1050:1079	The NF@HG exhibited enhanced antibacterial ability and the sustained release of TH can provide long-term antibacterial activity.
34492480	8	50	theme	biomedical	1460:1469	arg1	materials					1471:1479	biomedical materials	1460:1479	biomedical materials	1460:1479	Taken together, this strategy based on self-catalytic Fe3+/TA-CNF system may inspire new aspects on fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials.
34492480	3	51	theme	cold	520:523	arg1	temperature					525:535	room or cold temperature	512:535	temperature	525:535	Here, a series of hydrogels possessing ultrashort gelation time (~30 s) at room or cold temperature were fabricated based on self-catalytic Fe3+/Tannic acid-cellulose nanofiber (Fe3+/TA-CNF).
34492480	5	52	theme	hydrogel	848:855	arg1	ability					837:843	the antibacterial ability	819:843	the antibacterial ability of hydrogel	819:855	To improve the antibacterial ability of hydrogel, a bilayer hydrogel composite (NF@HG) composed of tetracycline hydrochloride (TH)-loaded electrospun nanofibers and hydrogel layer was fabricated via a mild casting method.
34492480	5	53	theme	bilayer	860:866	arg1	composite					877:885	a bilayer hydrogel composite	858:885	a bilayer hydrogel composite (NF@HG) composed of tetracycline hydrochloride (TH)-loaded electrospun nanofibers	858:967	To improve the antibacterial ability of hydrogel, a bilayer hydrogel composite (NF@HG) composed of tetracycline hydrochloride (TH)-loaded electrospun nanofibers and hydrogel layer was fabricated via a mild casting method.
34492480	5	53	theme	bilayer	860:866	arg1	HG					891:892	NF@HG	888:892	NF@HG	888:892	To improve the antibacterial ability of hydrogel, a bilayer hydrogel composite (NF@HG) composed of tetracycline hydrochloride (TH)-loaded electrospun nanofibers and hydrogel layer was fabricated via a mild casting method.
34492480	4	54	theme	ultrafast	761:769	arg1	polymerization					771:784	the ultrafast polymerization	757:784	the ultrafast polymerization of acrylic acid (AA)	757:805	Fe3+/TA-CNF formed stable redox pairs to activate ammonium persulfate (initiator), generating abundant free radicals to trigger the ultrafast polymerization of acrylic acid (AA).
34492480	7	55	theme	cell	1168:1171	arg1	results					1183:1189	cell viability results	1168:1189	cell viability results	1168:1189	Besides, cell viability results demonstrated that NF@HG was non-cytotoxic.
34492480	3	56	contain	possessing	465:474	arg2	~30 s					502:506	~30 s	502:506	~30 s	502:506	Here, a series of hydrogels possessing ultrashort gelation time (~30 s) at room or cold temperature were fabricated based on self-catalytic Fe3+/Tannic acid-cellulose nanofiber (Fe3+/TA-CNF).
34492480	3	56	contain	possessing	465:474	arg2	time					496:499	ultrashort gelation time	476:499	ultrashort gelation time (~30 s)	476:507	Here, a series of hydrogels possessing ultrashort gelation time (~30 s) at room or cold temperature were fabricated based on self-catalytic Fe3+/Tannic acid-cellulose nanofiber (Fe3+/TA-CNF).
34492480	3	56	contain	possessing	465:474	arg1	hydrogels					455:463	hydrogels	455:463	hydrogels possessing ultrashort gelation time (~30 s) at room or cold temperature	455:535	Here, a series of hydrogels possessing ultrashort gelation time (~30 s) at room or cold temperature were fabricated based on self-catalytic Fe3+/Tannic acid-cellulose nanofiber (Fe3+/TA-CNF).
34492480	5	57	theme	casting	1014:1020	arg1	method					1022:1027	a mild casting method	1007:1027	a mild casting method	1007:1027	To improve the antibacterial ability of hydrogel, a bilayer hydrogel composite (NF@HG) composed of tetracycline hydrochloride (TH)-loaded electrospun nanofibers and hydrogel layer was fabricated via a mild casting method.
34492480	8	58	theme	self-catalytic	1273:1286	arg1	system					1300:1305	self-catalytic Fe3+/TA-CNF system	1273:1305	self-catalytic Fe3+/TA-CNF system	1273:1305	Taken together, this strategy based on self-catalytic Fe3+/TA-CNF system may inspire new aspects on fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials.
34492480	2	59	theme	ambient	379:385	arg1	environment					387:397	ambient environment	379:397	ambient environment without stimuli	379:413	However, preparation of these hydrogels at ambient environment without stimuli is still a challenge.
34492480	8	60	theme	composites	1398:1407	arg1	preparation					1354:1364	fast and economical preparation	1334:1364	fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials	1334:1479	Taken together, this strategy based on self-catalytic Fe3+/TA-CNF system may inspire new aspects on fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials.
34492480	5	61	theme	mild	1009:1012	arg1	method					1022:1027	a mild casting method	1007:1027	a mild casting method	1007:1027	To improve the antibacterial ability of hydrogel, a bilayer hydrogel composite (NF@HG) composed of tetracycline hydrochloride (TH)-loaded electrospun nanofibers and hydrogel layer was fabricated via a mild casting method.
34492480	8	62	theme	hydrogels	1385:1393	arg1	preparation					1354:1364	fast and economical preparation	1334:1364	fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials	1334:1479	Taken together, this strategy based on self-catalytic Fe3+/TA-CNF system may inspire new aspects on fast and economical preparation of multifunctional hydrogels or composites, which have attractive industrial applications for biomedical materials.
34492480	6	63	theme	antibacterial	1135:1147	arg1	activity					1149:1156	long-term antibacterial activity	1125:1156	long-term antibacterial activity	1125:1156	The NF@HG exhibited enhanced antibacterial ability and the sustained release of TH can provide long-term antibacterial activity.
34492480	3	64	theme	room	512:515	arg1	temperature					525:535	room or cold temperature	512:535	temperature	525:535	Here, a series of hydrogels possessing ultrashort gelation time (~30 s) at room or cold temperature were fabricated based on self-catalytic Fe3+/Tannic acid-cellulose nanofiber (Fe3+/TA-CNF).
34492480	0	65	theme	acid-cellulose	93:106	arg1	nanofibers					108:117	self-catalytic Fe3+/Tannic acid-cellulose nanofibers	66:117	self-catalytic Fe3+/Tannic acid-cellulose nanofibers	66:117	Ultrafast gelation of multifunctional hydrogel/composite based on self-catalytic Fe3+/Tannic acid-cellulose nanofibers.
34492480	5	66	theme	@	890:890	arg1	composite					877:885	a bilayer hydrogel composite	858:885	a bilayer hydrogel composite (NF@HG) composed of tetracycline hydrochloride (TH)-loaded electrospun nanofibers	858:967	To improve the antibacterial ability of hydrogel, a bilayer hydrogel composite (NF@HG) composed of tetracycline hydrochloride (TH)-loaded electrospun nanofibers and hydrogel layer was fabricated via a mild casting method.
34492480	5	66	theme	@	890:890	arg1	HG					891:892	NF@HG	888:892	NF@HG	888:892	To improve the antibacterial ability of hydrogel, a bilayer hydrogel composite (NF@HG) composed of tetracycline hydrochloride (TH)-loaded electrospun nanofibers and hydrogel layer was fabricated via a mild casting method.
34492480	6	67	theme	long-term	1125:1133	arg1	activity					1149:1156	long-term antibacterial activity	1125:1156	long-term antibacterial activity	1125:1156	The NF@HG exhibited enhanced antibacterial ability and the sustained release of TH can provide long-term antibacterial activity.
34492480	1	68	theme	promising	274:282	arg1	hydrogels					136:144	Multifunctional hydrogels	120:144	Multifunctional hydrogels with transparency, ultraviolet (UV)-blocking, stretchable, self-healing, adhesive, antioxidant and antibacterial properties	120:268	Multifunctional hydrogels with transparency, ultraviolet (UV)-blocking, stretchable, self-healing, adhesive, antioxidant and antibacterial properties are promising materials for biomedical and relevant applications.
34492480	1	68	theme	promising	274:282	arg1	materials					284:292	promising materials	274:292	promising materials for biomedical and relevant applications	274:333	Multifunctional hydrogels with transparency, ultraviolet (UV)-blocking, stretchable, self-healing, adhesive, antioxidant and antibacterial properties are promising materials for biomedical and relevant applications.
34492480	0	69	theme	Fe3+/Tannic	81:91	arg1	nanofibers					108:117	self-catalytic Fe3+/Tannic acid-cellulose nanofibers	66:117	self-catalytic Fe3+/Tannic acid-cellulose nanofibers	66:117	Ultrafast gelation of multifunctional hydrogel/composite based on self-catalytic Fe3+/Tannic acid-cellulose nanofibers.
34492480	5	70	theme	hydrogel	868:875	arg1	composite					877:885	a bilayer hydrogel composite	858:885	a bilayer hydrogel composite (NF@HG) composed of tetracycline hydrochloride (TH)-loaded electrospun nanofibers	858:967	To improve the antibacterial ability of hydrogel, a bilayer hydrogel composite (NF@HG) composed of tetracycline hydrochloride (TH)-loaded electrospun nanofibers and hydrogel layer was fabricated via a mild casting method.
34492480	5	70	theme	hydrogel	868:875	arg1	HG					891:892	NF@HG	888:892	NF@HG	888:892	To improve the antibacterial ability of hydrogel, a bilayer hydrogel composite (NF@HG) composed of tetracycline hydrochloride (TH)-loaded electrospun nanofibers and hydrogel layer was fabricated via a mild casting method.
34492480	1	71	theme	-blocking	181:189	arg1	transparency					151:162	transparency	151:162	transparency	151:162	Multifunctional hydrogels with transparency, ultraviolet (UV)-blocking, stretchable, self-healing, adhesive, antioxidant and antibacterial properties are promising materials for biomedical and relevant applications.
34492480	7	72	theme	@	1211:1211	arg1	HG					1212:1213	NF@HG	1209:1213	NF@HG	1209:1213	Besides, cell viability results demonstrated that NF@HG was non-cytotoxic.
34492480	6	73	theme	NF	1034:1035	arg1	HG					1037:1038	The NF@HG	1030:1038	The NF@HG	1030:1038	The NF@HG exhibited enhanced antibacterial ability and the sustained release of TH can provide long-term antibacterial activity.
34056726	8	0	from	temperatures	1233:1244	arg1	crystals					1216:1223	crystals	1216:1223	crystals at high temperatures	1216:1244	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	8	1	theme	crystalline	1011:1021	arg1	lactose					1023:1029	crystalline lactose	1011:1029	crystalline lactose	1011:1029	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	10	2	from	lactose	1491:1497	arg1	system					1506:1511	the system	1502:1511	the system meant reducing sugar	1502:1532	An excess of lactose in the system meant reducing sugar declines were relatively small throughout.
34056726	4	3	theme	white	487:491	arg1	chocolates					493:502	five white chocolates	482:502	five white chocolates of varied compositions	482:525	In the present study, five white chocolates of varied compositions were sampled over time at different temperatures.
34056726	8	4	theme	lactose	1203:1209	arg1	MPI					1174:1176	MPI	1174:1176	MPI	1174:1176	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	8	4	theme	lactose	1203:1209	arg1	mobility					1191:1198	increased mobility	1181:1198	increased mobility of lactose	1181:1209	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	12	5	theme	reaction	1832:1839	arg1	rates					1841:1845	Maillard reaction rates	1823:1845	Maillard reaction rates	1823:1845	Both thickening rate and Maillard reaction rates were faster in chocolates with greater levels of amorphous lactose and when held at higher temperatures.
34056726	8	6	dep	NFDM	975:978	arg1	MPI					1003:1005	MPI	1003:1005	MPI	1003:1005	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	8	6	dep	NFDM	975:978	arg1	isolate					994:1000	milk protein isolate	981:1000	milk protein isolate	981:1000	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	8	6	dep	NFDM	975:978	arg1	lactose					1023:1029	crystalline lactose	1011:1029	crystalline lactose	1011:1029	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	13	7	theme	PRACTICAL	1952:1960	arg1	APPLICATION					1962:1972	PRACTICAL APPLICATION	1952:1972	PRACTICAL APPLICATION: So-called "golden chocolate" is white chocolate that has been held at warm temperatures for a period of time to undergo Maillard browning.	1952:2112	PRACTICAL APPLICATION: So-called "golden chocolate" is white chocolate that has been held at warm temperatures for a period of time to undergo Maillard browning.
34056726	10	8	theme	lactose	1491:1497	arg1	excess					1481:1486	An excess	1478:1486	An excess of lactose in the system meant reducing sugar	1478:1532	An excess of lactose in the system meant reducing sugar declines were relatively small throughout.
34056726	1	9	theme	time	181:184	arg1	periods					170:176	extended periods	161:176	extended periods of time	161:184	Molten white chocolate held at high temperatures for extended periods of time undergoes thickening and nonenzymatic browning reactions.
34056726	8	10	theme	high	1228:1231	arg1	temperatures					1233:1244	high temperatures	1228:1244	high temperatures	1228:1244	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	9	11	theme	end	1402:1404	arg1	products					1412:1419	colored end stage products	1394:1419	colored end stage products	1394:1419	The evolution of fluorescent intermediates was visualized using fluorescence spectroscopy, revealing that these intermediates were formed prior to colored end stage products and were consumed as the reaction approaches completion.
34056726	14	12	theme	chocolate	2141:2149	arg1	thickening					2123:2132	thickening	2123:2132	thickening of the chocolate during heating	2123:2164	However, thickening of the chocolate during heating can cause processing problems.
34056726	9	13	theme	intermediates	1276:1288	arg1	evolution					1251:1259	The evolution	1247:1259	The evolution of fluorescent intermediates	1247:1288	The evolution of fluorescent intermediates was visualized using fluorescence spectroscopy, revealing that these intermediates were formed prior to colored end stage products and were consumed as the reaction approaches completion.
34056726	1	14	theme	nonenzymatic	211:222	arg1	reactions					233:241	nonenzymatic browning reactions	211:241	nonenzymatic browning reactions	211:241	Molten white chocolate held at high temperatures for extended periods of time undergoes thickening and nonenzymatic browning reactions.
34056726	10	15	from	system	1506:1511	arg1	excess					1481:1486	An excess	1478:1486	An excess of lactose in the system meant reducing sugar	1478:1532	An excess of lactose in the system meant reducing sugar declines were relatively small throughout.
34056726	5	16	with	chocolates	668:677	arg1	crystalline					684:694	crystalline	684:694	crystalline	684:694	White chocolates containing amorphous lactose showed increases in complex viscosity, while chocolates with crystalline or no lactose showed no change in rheological properties.
34056726	5	16	with	chocolates	668:677	arg1	lactose					702:708	no lactose	699:708	no lactose	699:708	White chocolates containing amorphous lactose showed increases in complex viscosity, while chocolates with crystalline or no lactose showed no change in rheological properties.
34056726	9	17	dep	approaches	1455:1464	arg1	completion					1466:1475	completion	1466:1475	the reaction approaches completion	1442:1475	The evolution of fluorescent intermediates was visualized using fluorescence spectroscopy, revealing that these intermediates were formed prior to colored end stage products and were consumed as the reaction approaches completion.
34056726	5	18	theme	rheological	730:740	arg1	properties					742:751	rheological properties	730:751	rheological properties	730:751	White chocolates containing amorphous lactose showed increases in complex viscosity, while chocolates with crystalline or no lactose showed no change in rheological properties.
34056726	9	19	theme	fluorescence	1311:1322	arg1	spectroscopy					1324:1335	fluorescence spectroscopy	1311:1335	fluorescence spectroscopy	1311:1335	The evolution of fluorescent intermediates was visualized using fluorescence spectroscopy, revealing that these intermediates were formed prior to colored end stage products and were consumed as the reaction approaches completion.
34056726	5	20	theme	amorphous	605:613	arg1	lactose					615:621	amorphous lactose	605:621	amorphous lactose	605:621	White chocolates containing amorphous lactose showed increases in complex viscosity, while chocolates with crystalline or no lactose showed no change in rheological properties.
34056726	5	21	contain	containing	594:603	arg2	lactose					615:621	amorphous lactose	605:621	amorphous lactose	605:621	White chocolates containing amorphous lactose showed increases in complex viscosity, while chocolates with crystalline or no lactose showed no change in rheological properties.
34056726	5	21	contain	containing	594:603	arg1	chocolates					583:592	White chocolates	577:592	White chocolates containing amorphous lactose	577:621	White chocolates containing amorphous lactose showed increases in complex viscosity, while chocolates with crystalline or no lactose showed no change in rheological properties.
34056726	13	22	theme	chocolate	1993:2001	arg1	"					2002:2002	So-called "golden chocolate"	1975:2002	So-called "golden chocolate"	1975:2002	PRACTICAL APPLICATION: So-called "golden chocolate" is white chocolate that has been held at warm temperatures for a period of time to undergo Maillard browning.
34056726	5	23	from	increases	630:638	arg1	viscosity					651:659	complex viscosity	643:659	complex viscosity	643:659	White chocolates containing amorphous lactose showed increases in complex viscosity, while chocolates with crystalline or no lactose showed no change in rheological properties.
34056726	4	24	theme	present	467:473	arg1	study					475:479	the present study	463:479	the present study	463:479	In the present study, five white chocolates of varied compositions were sampled over time at different temperatures.
34056726	10	25	dep	declines	1534:1541	arg1	small					1559:1563	small	1559:1563	small	1559:1563	An excess of lactose in the system meant reducing sugar declines were relatively small throughout.
34056726	10	26	theme	meant	1513:1517	arg1	system					1506:1511	the system	1502:1511	the system meant reducing sugar	1502:1532	An excess of lactose in the system meant reducing sugar declines were relatively small throughout.
34056726	11	27	from	influence	1622:1630	arg1	thickening					1676:1685	thickening	1676:1685	thickening as related to complex viscosity (first order) and browning via colorimetry and absorbance (pseudo-zero order)	1676:1795	Kinetic models were created to determine the influence of chocolate composition and temperature on thickening as related to complex viscosity (first order) and browning via colorimetry and absorbance (pseudo-zero order).
34056726	14	28	theme	processing	2176:2185	arg1	problems					2187:2194	processing problems	2176:2194	processing problems	2176:2194	However, thickening of the chocolate during heating can cause processing problems.
34056726	13	29	theme	Maillard	2095:2102	arg1	browning					2104:2111	Maillard browning	2095:2111	Maillard browning	2095:2111	PRACTICAL APPLICATION: So-called "golden chocolate" is white chocolate that has been held at warm temperatures for a period of time to undergo Maillard browning.
34056726	1	30	theme	high	139:142	arg1	temperatures					144:155	high temperatures	139:155	high temperatures	139:155	Molten white chocolate held at high temperatures for extended periods of time undergoes thickening and nonenzymatic browning reactions.
34056726	3	31	theme	amorphous	373:381	arg1	lactose					383:389	amorphous lactose	373:389	amorphous lactose	373:389	Crystallization of amorphous lactose and high temperature contribute to rheological changes over heating.
34056726	0	32	from	changes	58:64	arg1	chocolate					75:83	chocolate	75:83	chocolate	75:83	Characterizing Maillard reaction kinetics and rheological changes in white chocolate over extended heating.
34056726	4	33	theme	compositions	514:525	arg1	chocolates					493:502	five white chocolates	482:502	five white chocolates of varied compositions	482:525	In the present study, five white chocolates of varied compositions were sampled over time at different temperatures.
34056726	6	34	theme	Maillard	754:761	arg1	browning					763:770	Maillard browning	754:770	Maillard browning	754:770	Maillard browning was measured through both colorimetry and spectrophotometric analysis of brown pigments.
34056726	7	35	theme	greatest	885:892	arg1	browning					894:901	the greatest browning	881:901	the greatest browning for Nonfat dry milk (NFDM)-containing systems	881:947	Both markers showed the greatest browning for Nonfat dry milk (NFDM)-containing systems.
34056726	3	36	theme	temperature	400:410	arg1	Crystallization					354:368	Crystallization	354:368	Crystallization of amorphous lactose and high temperature	354:410	Crystallization of amorphous lactose and high temperature contribute to rheological changes over heating.
34056726	0	37	theme	reaction	24:31	arg1	kinetics					33:40	Maillard reaction kinetics	15:40	Maillard reaction kinetics	15:40	Characterizing Maillard reaction kinetics and rheological changes in white chocolate over extended heating.
34056726	12	38	theme	higher	1931:1936	arg1	temperatures					1938:1949	higher temperatures	1931:1949	higher temperatures	1931:1949	Both thickening rate and Maillard reaction rates were faster in chocolates with greater levels of amorphous lactose and when held at higher temperatures.
34056726	8	39	theme	milk	981:984	arg1	isolate					994:1000	milk protein isolate	981:1000	milk protein isolate	981:1000	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	11	40	theme	pseudo-zero	1778:1788	arg1	order					1790:1794	pseudo-zero order	1778:1794	pseudo-zero order	1778:1794	Kinetic models were created to determine the influence of chocolate composition and temperature on thickening as related to complex viscosity (first order) and browning via colorimetry and absorbance (pseudo-zero order).
34056726	11	40	theme	pseudo-zero	1778:1788	arg1	colorimetry					1750:1760	colorimetry	1750:1760	colorimetry	1750:1760	Kinetic models were created to determine the influence of chocolate composition and temperature on thickening as related to complex viscosity (first order) and browning via colorimetry and absorbance (pseudo-zero order).
34056726	4	41	theme	different	553:561	arg1	temperatures					563:574	different temperatures	553:574	different temperatures	553:574	In the present study, five white chocolates of varied compositions were sampled over time at different temperatures.
34056726	13	42	theme	time	2079:2082	arg1	period					2069:2074	a period	2067:2074	a period of time to undergo Maillard browning	2067:2111	PRACTICAL APPLICATION: So-called "golden chocolate" is white chocolate that has been held at warm temperatures for a period of time to undergo Maillard browning.
34056726	8	43	theme	amorphous	1149:1157	arg1	lactose					1159:1165	residual amorphous lactose	1140:1165	residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures	1140:1244	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	8	44	from	crystals	1216:1223	arg1	lactose					1159:1165	residual amorphous lactose	1140:1165	residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures	1140:1244	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	8	44	from	crystals	1216:1223	arg1	MPI					1174:1176	MPI	1174:1176	MPI	1174:1176	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	8	44	from	crystals	1216:1223	arg1	mobility					1191:1198	increased mobility	1181:1198	increased mobility of lactose	1181:1209	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	12	45	theme	lactose	1906:1912	arg1	levels					1886:1891	greater levels	1878:1891	greater levels of amorphous lactose	1878:1912	Both thickening rate and Maillard reaction rates were faster in chocolates with greater levels of amorphous lactose and when held at higher temperatures.
34056726	11	46	theme	composition	1645:1655	arg1	influence					1622:1630	the influence	1618:1630	the influence of chocolate composition and temperature on thickening as related to complex viscosity (first order) and browning via colorimetry and absorbance (pseudo-zero order)	1618:1795	Kinetic models were created to determine the influence of chocolate composition and temperature on thickening as related to complex viscosity (first order) and browning via colorimetry and absorbance (pseudo-zero order).
34056726	6	47	theme	spectrophotometric	814:831	arg1	analysis					833:840	spectrophotometric analysis	814:840	spectrophotometric analysis of brown pigments	814:858	Maillard browning was measured through both colorimetry and spectrophotometric analysis of brown pigments.
34056726	9	48	theme	reaction	1446:1453	arg1	intermediates					1359:1371	these intermediates	1353:1371	these intermediates	1353:1371	The evolution of fluorescent intermediates was visualized using fluorescence spectroscopy, revealing that these intermediates were formed prior to colored end stage products and were consumed as the reaction approaches completion.
34056726	9	48	theme	reaction	1446:1453	arg1	approaches					1455:1464	the reaction approaches completion	1442:1475	the reaction approaches completion	1442:1475	The evolution of fluorescent intermediates was visualized using fluorescence spectroscopy, revealing that these intermediates were formed prior to colored end stage products and were consumed as the reaction approaches completion.
34056726	12	49	theme	greater	1878:1884	arg1	levels					1886:1891	greater levels	1878:1891	greater levels of amorphous lactose	1878:1912	Both thickening rate and Maillard reaction rates were faster in chocolates with greater levels of amorphous lactose and when held at higher temperatures.
34056726	8	50	theme	increased	1181:1189	arg1	mobility					1191:1198	increased mobility	1181:1198	increased mobility of lactose	1181:1209	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	11	51	theme	first	1720:1724	arg1	order					1726:1730	first order	1720:1730	first order	1720:1730	Kinetic models were created to determine the influence of chocolate composition and temperature on thickening as related to complex viscosity (first order) and browning via colorimetry and absorbance (pseudo-zero order).
34056726	11	51	theme	first	1720:1724	arg1	viscosity					1709:1717	complex viscosity	1701:1717	complex viscosity (first order)	1701:1731	Kinetic models were created to determine the influence of chocolate composition and temperature on thickening as related to complex viscosity (first order) and browning via colorimetry and absorbance (pseudo-zero order).
34056726	8	52	contain	containing	961:970	arg1	Chocolates					950:959	Chocolates	950:959	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose)	950:1030	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	8	52	contain	containing	961:970	arg2	NFDM					975:978	no NFDM	972:978	no NFDM (milk protein isolate, MPI, or crystalline lactose)	972:1030	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	13	53	theme	warm	2045:2048	arg1	temperatures					2050:2061	warm temperatures	2045:2061	warm temperatures	2045:2061	PRACTICAL APPLICATION: So-called "golden chocolate" is white chocolate that has been held at warm temperatures for a period of time to undergo Maillard browning.
34056726	7	54	theme	-containing	929:939	arg1	systems					941:947	Nonfat dry milk (NFDM)-containing systems	907:947	Nonfat dry milk (NFDM)-containing systems	907:947	Both markers showed the greatest browning for Nonfat dry milk (NFDM)-containing systems.
34056726	2	55	theme	higher	333:338	arg1	temperatures					340:351	higher temperatures	333:351	higher temperatures	333:351	Lactose and milk proteins participate in Maillard browning, which occurs more rapidly at higher temperatures.
34056726	15	56	theme	viscosity	2212:2220	arg1	changes					2222:2228	viscosity changes	2212:2228	viscosity changes	2212:2228	In this study, viscosity changes are correlated with Maillard browning kinetics to help chocolate manufacturers find optimal conditions for creating new products.
34056726	8	57	from	browning	1093:1100	arg1	results					1117:1123	colorimetry results	1105:1123	colorimetry results	1105:1123	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	12	58	theme	Maillard	1823:1830	arg1	rates					1841:1845	Maillard reaction rates	1823:1845	Maillard reaction rates	1823:1845	Both thickening rate and Maillard reaction rates were faster in chocolates with greater levels of amorphous lactose and when held at higher temperatures.
34056726	13	59	dep	APPLICATION	1962:1972	arg1	chocolate					2013:2021	chocolate	2013:2021	chocolate	2013:2021	PRACTICAL APPLICATION: So-called "golden chocolate" is white chocolate that has been held at warm temperatures for a period of time to undergo Maillard browning.
34056726	9	60	theme	fluorescent	1264:1274	arg1	intermediates					1276:1288	fluorescent intermediates	1264:1288	fluorescent intermediates	1264:1288	The evolution of fluorescent intermediates was visualized using fluorescence spectroscopy, revealing that these intermediates were formed prior to colored end stage products and were consumed as the reaction approaches completion.
34056726	15	61	theme	Maillard	2250:2257	arg1	kinetics					2268:2275	Maillard browning kinetics	2250:2275	Maillard browning kinetics to help chocolate manufacturers find optimal conditions for creating new products	2250:2357	In this study, viscosity changes are correlated with Maillard browning kinetics to help chocolate manufacturers find optimal conditions for creating new products.
34056726	13	62	theme	So-called	1975:1983	arg1	"					2002:2002	So-called "golden chocolate"	1975:2002	So-called "golden chocolate"	1975:2002	PRACTICAL APPLICATION: So-called "golden chocolate" is white chocolate that has been held at warm temperatures for a period of time to undergo Maillard browning.
34056726	2	63	theme	milk	256:259	arg1	proteins					261:268	Lactose and milk proteins	244:268	Lactose and milk proteins	244:268	Lactose and milk proteins participate in Maillard browning, which occurs more rapidly at higher temperatures.
34056726	1	64	theme	browning	224:231	arg1	reactions					233:241	nonenzymatic browning reactions	211:241	nonenzymatic browning reactions	211:241	Molten white chocolate held at high temperatures for extended periods of time undergoes thickening and nonenzymatic browning reactions.
34056726	2	65	theme	Lactose	244:250	arg1	proteins					261:268	Lactose and milk proteins	244:268	Lactose and milk proteins	244:268	Lactose and milk proteins participate in Maillard browning, which occurs more rapidly at higher temperatures.
34056726	15	66	theme	chocolate	2285:2293	arg1	manufacturers					2295:2307	chocolate manufacturers	2285:2307	chocolate manufacturers	2285:2307	In this study, viscosity changes are correlated with Maillard browning kinetics to help chocolate manufacturers find optimal conditions for creating new products.
34056726	13	67	theme	golden	1986:1991	arg1	"					2002:2002	So-called "golden chocolate"	1975:2002	So-called "golden chocolate"	1975:2002	PRACTICAL APPLICATION: So-called "golden chocolate" is white chocolate that has been held at warm temperatures for a period of time to undergo Maillard browning.
34056726	6	68	theme	pigments	851:858	arg1	colorimetry					798:808	colorimetry	798:808	colorimetry	798:808	Maillard browning was measured through both colorimetry and spectrophotometric analysis of brown pigments.
34056726	6	68	theme	pigments	851:858	arg1	analysis					833:840	spectrophotometric analysis	814:840	spectrophotometric analysis of brown pigments	814:858	Maillard browning was measured through both colorimetry and spectrophotometric analysis of brown pigments.
34056726	2	69	theme	Maillard	285:292	arg1	browning					294:301	Maillard browning	285:301	Maillard browning	285:301	Lactose and milk proteins participate in Maillard browning, which occurs more rapidly at higher temperatures.
34056726	15	70	theme	browning	2259:2266	arg1	kinetics					2268:2275	Maillard browning kinetics	2250:2275	Maillard browning kinetics to help chocolate manufacturers find optimal conditions for creating new products	2250:2357	In this study, viscosity changes are correlated with Maillard browning kinetics to help chocolate manufacturers find optimal conditions for creating new products.
34056726	0	71	from	kinetics	33:40	arg1	chocolate					75:83	chocolate	75:83	chocolate	75:83	Characterizing Maillard reaction kinetics and rheological changes in white chocolate over extended heating.
34056726	0	72	theme	extended	90:97	arg1	heating					99:105	extended heating	90:105	extended heating	90:105	Characterizing Maillard reaction kinetics and rheological changes in white chocolate over extended heating.
34056726	5	73	theme	White	577:581	arg1	chocolates					583:592	White chocolates	577:592	White chocolates containing amorphous lactose	577:621	White chocolates containing amorphous lactose showed increases in complex viscosity, while chocolates with crystalline or no lactose showed no change in rheological properties.
34056726	1	74	theme	extended	161:168	arg1	periods					170:176	extended periods	161:176	extended periods of time	161:184	Molten white chocolate held at high temperatures for extended periods of time undergoes thickening and nonenzymatic browning reactions.
34056726	11	75	theme	Kinetic	1577:1583	arg1	models					1585:1590	Kinetic models	1577:1590	Kinetic models	1577:1590	Kinetic models were created to determine the influence of chocolate composition and temperature on thickening as related to complex viscosity (first order) and browning via colorimetry and absorbance (pseudo-zero order).
34056726	15	76	dep	kinetics	2268:2275	arg1	help					2280:2283	help	2280:2283	to help chocolate manufacturers find optimal conditions for creating new products	2277:2357	In this study, viscosity changes are correlated with Maillard browning kinetics to help chocolate manufacturers find optimal conditions for creating new products.
34056726	8	77	from	lactose	1159:1165	arg1	MPI					1174:1176	MPI	1174:1176	MPI	1174:1176	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	8	77	from	lactose	1159:1165	arg1	mobility					1191:1198	increased mobility	1181:1198	increased mobility of lactose	1181:1209	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	8	77	from	lactose	1159:1165	arg1	crystals					1216:1223	crystals	1216:1223	crystals at high temperatures	1216:1244	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	3	78	theme	high	395:398	arg1	temperature					400:410	high temperature	395:410	high temperature	395:410	Crystallization of amorphous lactose and high temperature contribute to rheological changes over heating.
34056726	4	79	theme	varied	507:512	arg1	compositions					514:525	varied compositions	507:525	varied compositions	507:525	In the present study, five white chocolates of varied compositions were sampled over time at different temperatures.
34056726	8	80	theme	colorimetry	1105:1115	arg1	results					1117:1123	colorimetry results	1105:1123	colorimetry results	1105:1123	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	3	81	theme	lactose	383:389	arg1	Crystallization					354:368	Crystallization	354:368	Crystallization of amorphous lactose and high temperature	354:410	Crystallization of amorphous lactose and high temperature contribute to rheological changes over heating.
34056726	0	82	theme	Maillard	15:22	arg1	kinetics					33:40	Maillard reaction kinetics	15:40	Maillard reaction kinetics	15:40	Characterizing Maillard reaction kinetics and rheological changes in white chocolate over extended heating.
34056726	9	83	theme	colored	1394:1400	arg1	products					1412:1419	colored end stage products	1394:1419	colored end stage products	1394:1419	The evolution of fluorescent intermediates was visualized using fluorescence spectroscopy, revealing that these intermediates were formed prior to colored end stage products and were consumed as the reaction approaches completion.
34056726	3	84	theme	rheological	426:436	arg1	changes					438:444	rheological changes	426:444	rheological changes over heating	426:457	Crystallization of amorphous lactose and high temperature contribute to rheological changes over heating.
34056726	8	85	dep	MPI	1174:1176	arg1	the					1170:1172	the	1170:1172	the	1170:1172	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	9	86	theme	stage	1406:1410	arg1	products					1412:1419	colored end stage products	1394:1419	colored end stage products	1394:1419	The evolution of fluorescent intermediates was visualized using fluorescence spectroscopy, revealing that these intermediates were formed prior to colored end stage products and were consumed as the reaction approaches completion.
34056726	5	87	theme	complex	643:649	arg1	viscosity					651:659	complex viscosity	643:659	complex viscosity	643:659	White chocolates containing amorphous lactose showed increases in complex viscosity, while chocolates with crystalline or no lactose showed no change in rheological properties.
34056726	0	88	theme	rheological	46:56	arg1	changes					58:64	rheological changes	46:64	rheological changes in white chocolate over extended heating	46:105	Characterizing Maillard reaction kinetics and rheological changes in white chocolate over extended heating.
34056726	15	89	theme	new	2346:2348	arg1	products					2350:2357	new products	2346:2357	new products	2346:2357	In this study, viscosity changes are correlated with Maillard browning kinetics to help chocolate manufacturers find optimal conditions for creating new products.
34056726	8	90	theme	residual	1140:1147	arg1	lactose					1159:1165	residual amorphous lactose	1140:1165	residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures	1140:1244	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	12	91	with	chocolates	1862:1871	arg1	levels					1886:1891	greater levels	1878:1891	greater levels of amorphous lactose	1878:1912	Both thickening rate and Maillard reaction rates were faster in chocolates with greater levels of amorphous lactose and when held at higher temperatures.
34056726	6	92	theme	brown	845:849	arg1	pigments					851:858	brown pigments	845:858	brown pigments	845:858	Maillard browning was measured through both colorimetry and spectrophotometric analysis of brown pigments.
34056726	5	93	from	change	720:725	arg1	properties					742:751	rheological properties	730:751	rheological properties	730:751	White chocolates containing amorphous lactose showed increases in complex viscosity, while chocolates with crystalline or no lactose showed no change in rheological properties.
34056726	8	94	theme	protein	986:992	arg1	isolate					994:1000	milk protein isolate	981:1000	milk protein isolate	981:1000	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	8	95	from	change	1042:1047	arg1	absorbance					1052:1061	absorbance	1052:1061	absorbance after clarification	1052:1081	Chocolates containing no NFDM (milk protein isolate, MPI, or crystalline lactose) showed no change in absorbance after clarification, but some browning in colorimetry results, likely due to residual amorphous lactose in the MPI or increased mobility of lactose from crystals at high temperatures.
34056726	11	96	theme	temperature	1661:1671	arg1	influence					1622:1630	the influence	1618:1630	the influence of chocolate composition and temperature on thickening as related to complex viscosity (first order) and browning via colorimetry and absorbance (pseudo-zero order)	1618:1795	Kinetic models were created to determine the influence of chocolate composition and temperature on thickening as related to complex viscosity (first order) and browning via colorimetry and absorbance (pseudo-zero order).
34056726	15	97	theme	optimal	2314:2320	arg1	conditions					2322:2331	optimal conditions	2314:2331	optimal conditions	2314:2331	In this study, viscosity changes are correlated with Maillard browning kinetics to help chocolate manufacturers find optimal conditions for creating new products.
34056726	12	98	theme	amorphous	1896:1904	arg1	lactose					1906:1912	amorphous lactose	1896:1912	amorphous lactose	1896:1912	Both thickening rate and Maillard reaction rates were faster in chocolates with greater levels of amorphous lactose and when held at higher temperatures.
34056726	11	99	theme	chocolate	1635:1643	arg1	composition					1645:1655	chocolate composition	1635:1655	chocolate composition	1635:1655	Kinetic models were created to determine the influence of chocolate composition and temperature on thickening as related to complex viscosity (first order) and browning via colorimetry and absorbance (pseudo-zero order).
34056726	11	100	theme	complex	1701:1707	arg1	order					1726:1730	first order	1720:1730	first order	1720:1730	Kinetic models were created to determine the influence of chocolate composition and temperature on thickening as related to complex viscosity (first order) and browning via colorimetry and absorbance (pseudo-zero order).
34056726	11	100	theme	complex	1701:1707	arg1	viscosity					1709:1717	complex viscosity	1701:1717	complex viscosity (first order)	1701:1731	Kinetic models were created to determine the influence of chocolate composition and temperature on thickening as related to complex viscosity (first order) and browning via colorimetry and absorbance (pseudo-zero order).
34056726	10	101	from	excess	1481:1486	arg1	system					1506:1511	the system	1502:1511	the system meant reducing sugar	1502:1532	An excess of lactose in the system meant reducing sugar declines were relatively small throughout.
34056726	11	102	theme	related	1690:1696	arg1	thickening					1676:1685	thickening	1676:1685	thickening as related to complex viscosity (first order) and browning via colorimetry and absorbance (pseudo-zero order)	1676:1795	Kinetic models were created to determine the influence of chocolate composition and temperature on thickening as related to complex viscosity (first order) and browning via colorimetry and absorbance (pseudo-zero order).
34056726	12	103	theme	thickening	1803:1812	arg1	rate					1814:1817	thickening rate	1803:1817	thickening rate	1803:1817	Both thickening rate and Maillard reaction rates were faster in chocolates with greater levels of amorphous lactose and when held at higher temperatures.
33398638	4	0	theme	acid	535:538	arg1	-GAGs					544:548	Hyaluronic acid (HA)-GAGs	524:548	Hyaluronic acid (HA)-GAGs	524:548	Hyaluronic acid (HA)-GAGs are not bound to core proteins.
33398638	1	1	theme	brain	246:250	arg1	plasticity					217:226	the plasticity	213:226	the plasticity of the developing brain	213:250	Astrocytes are major producers of the extracellular matrix (ECM), which is involved in the plasticity of the developing brain.
33398638	7	2	theme	brain	1003:1007	arg1	ECM					1009:1011	the brain ECM	999:1011	the brain ECM	999:1011	Finally, we investigated the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM, in vivo using translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice.
33398638	10	3	theme	brain-specific	1431:1444	arg1	lecticans					1446:1454	brain-specific lecticans	1431:1454	C4S-GAGs as well as brain-specific lecticans neurocan and brevican	1411:1476	Ethanol upregulates C4S-GAGs as well as brain-specific lecticans neurocan and brevican, which are highly enriched in astrocytes of the developing cortex in vivo.
33398638	10	3	theme	brain-specific	1431:1444	arg1	brevican					1469:1476	brevican	1469:1476	brevican	1469:1476	Ethanol upregulates C4S-GAGs as well as brain-specific lecticans neurocan and brevican, which are highly enriched in astrocytes of the developing cortex in vivo.
33398638	10	3	theme	brain-specific	1431:1444	arg1	neurocan					1456:1463	neurocan	1456:1463	neurocan	1456:1463	Ethanol upregulates C4S-GAGs as well as brain-specific lecticans neurocan and brevican, which are highly enriched in astrocytes of the developing cortex in vivo.
33398638	9	4	theme	chondroitin-0-sulfate	1286:1306	arg1	-GAGs					1313:1317	chondroitin-0-sulfate (C0S)-GAGs	1286:1317	chondroitin-0-sulfate (C0S)-GAGs (possibly because of a higher sulfatase enzyme expression)	1286:1376	Astrocytes have high levels of chondroitin-0-sulfate (C0S)-GAGs (possibly because of a higher sulfatase enzyme expression) and HS-GAGs.
33398638	2	5	dep	In	253:254	arg1	utero					256:260	utero	256:260	utero	256:260	In utero alcohol exposure alters neuronal plasticity.
33398638	7	6	theme	major	979:983	arg1	components					985:994	major components	979:994	major components of the brain ECM	979:1011	Finally, we investigated the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM, in vivo using translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice.
33398638	7	6	theme	major	979:983	arg1	proteins					969:976	proteins	969:976	proteins	969:976	Finally, we investigated the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM, in vivo using translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice.
33398638	9	7	theme	-GAGs	1313:1317	arg1	levels					1276:1281	high levels	1271:1281	high levels of chondroitin-0-sulfate (C0S)-GAGs (possibly because of a higher sulfatase enzyme expression) and HS-GAGs	1271:1388	Astrocytes have high levels of chondroitin-0-sulfate (C0S)-GAGs (possibly because of a higher sulfatase enzyme expression) and HS-GAGs.
33398638	8	8	theme	HA	1150:1151	arg1	levels					1140:1145	low levels	1136:1145	low levels of HA	1136:1151	Cortical astrocytes produce low levels of HA and show low expression of genes involved in HA biosynthesis compared to the whole developing cortex.
33398638	7	9	theme	proteins	969:976	arg1	family					959:964	the lectican family	946:964	the lectican family of proteins, major components of the brain ECM,	946:1012	Finally, we investigated the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM, in vivo using translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice.
33398638	2	10	theme	neuronal	286:293	arg1	plasticity					295:304	neuronal plasticity	286:304	neuronal plasticity	286:304	In utero alcohol exposure alters neuronal plasticity.
33398638	7	11	theme	family	959:964	arg1	CS-PGs					936:941	CS-PGs	936:941	CS-PGs of the lectican family of proteins, major components of the brain ECM,	936:1012	Finally, we investigated the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM, in vivo using translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice.
33398638	5	12	theme	cortical	719:726	arg1	cultures					738:745	cortical astrocyte cultures	719:745	cortical astrocyte cultures	719:745	In this study we investigated the contribution of astrocytes to CS-, HS-, and HA-GAG production by comparing the makeup of these GAGs in cortical astrocyte cultures and the neonatal rat cortex.
33398638	9	13	theme	sulfatase	1349:1357	arg1	expression					1366:1375	a higher sulfatase enzyme expression	1340:1375	a higher sulfatase enzyme expression	1340:1375	Astrocytes have high levels of chondroitin-0-sulfate (C0S)-GAGs (possibly because of a higher sulfatase enzyme expression) and HS-GAGs.
33398638	8	14	theme	Cortical	1108:1115	arg1	astrocytes					1117:1126	Cortical astrocytes	1108:1126	Cortical astrocytes	1108:1126	Cortical astrocytes produce low levels of HA and show low expression of genes involved in HA biosynthesis compared to the whole developing cortex.
33398638	6	15	theme	protein	840:846	arg1	levels					848:853	GAG and core protein levels	827:853	GAG and core protein levels in astrocytes	827:867	We also explored alterations induced by ethanol in GAG and core protein levels in astrocytes.
33398638	0	16	from	Cultures	84:91	arg1	Characterization					0:15	Characterization	0:15	Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures	0:91	Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures and the Cortex of Neonatal Rats.
33398638	0	16	from	Cultures	84:91	arg1	Cortex					101:106	the Cortex	97:106	the Cortex of Neonatal Rats	97:123	Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures and the Cortex of Neonatal Rats.
33398638	11	17	theme	ethanol-induced	1670:1684	arg1	alterations					1686:1696	ethanol-induced alterations	1670:1696	ethanol-induced alterations of neuronal development	1670:1720	These results begin to elucidate the role of astrocytes in the biosynthesis of CS- HS- and HA-GAGs, and suggest that ethanol-induced alterations of neuronal development may be in part mediated by increased astrocyte GAG levels and neurocan and brevican expression.
33398638	7	18	theme	relative	899:906	arg1	expression					908:917	the relative expression	895:917	the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM,	895:1012	Finally, we investigated the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM, in vivo using translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice.
33398638	8	19	theme	genes	1180:1184	arg1	expression					1166:1175	low expression	1162:1175	low expression of genes involved in HA biosynthesis compared to the whole developing cortex	1162:1252	Cortical astrocytes produce low levels of HA and show low expression of genes involved in HA biosynthesis compared to the whole developing cortex.
33398638	7	20	theme	CS-PGs	936:941	arg1	expression					908:917	the relative expression	895:917	the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM,	895:1012	Finally, we investigated the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM, in vivo using translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice.
33398638	3	21	attach	present	365:371	arg2	polysaccharides					349:363	polysaccharides	349:363	polysaccharides present in the extracellular space	349:398	Glycosaminoglycans (GAGs) are a family of polysaccharides present in the extracellular space; chondroitin sulfate (CS)- and heparan sulfate (HS)-GAGs are covalently bound to core proteins to form proteoglycans (PGs).
33398638	3	21	attach	present	365:371	arg1	space					394:398	the extracellular space	376:398	the extracellular space	376:398	Glycosaminoglycans (GAGs) are a family of polysaccharides present in the extracellular space; chondroitin sulfate (CS)- and heparan sulfate (HS)-GAGs are covalently bound to core proteins to form proteoglycans (PGs).
33398638	11	22	theme	brevican	1797:1804	arg1	expression					1806:1815	neurocan and brevican expression	1784:1815	neurocan and brevican expression	1784:1815	These results begin to elucidate the role of astrocytes in the biosynthesis of CS- HS- and HA-GAGs, and suggest that ethanol-induced alterations of neuronal development may be in part mediated by increased astrocyte GAG levels and neurocan and brevican expression.
33398638	3	23	from	space	394:398	arg1	present					365:371	present	365:371	present	365:371	Glycosaminoglycans (GAGs) are a family of polysaccharides present in the extracellular space; chondroitin sulfate (CS)- and heparan sulfate (HS)-GAGs are covalently bound to core proteins to form proteoglycans (PGs).
33398638	0	24	theme	Glycosaminoglycan	20:36	arg1	Composition					51:61	Glycosaminoglycan Disaccharide Composition	20:61	Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures	20:91	Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures and the Cortex of Neonatal Rats.
33398638	1	25	theme	matrix	178:183	arg1	Astrocytes					126:135	Astrocytes	126:135	Astrocytes	126:135	Astrocytes are major producers of the extracellular matrix (ECM), which is involved in the plasticity of the developing brain.
33398638	1	25	theme	matrix	178:183	arg1	producers					147:155	major producers	141:155	major producers of the extracellular matrix (ECM), which is involved in the plasticity of the developing brain	141:250	Astrocytes are major producers of the extracellular matrix (ECM), which is involved in the plasticity of the developing brain.
33398638	0	26	from	Characterization	0:15	arg1	Cultures					84:91	Astrocyte Primary Cultures	66:91	Astrocyte Primary Cultures	66:91	Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures and the Cortex of Neonatal Rats.
33398638	11	27	theme	CS-	1632:1634	arg1	HS-					1636:1638	CS- HS-	1632:1638	CS- HS-	1632:1638	These results begin to elucidate the role of astrocytes in the biosynthesis of CS- HS- and HA-GAGs, and suggest that ethanol-induced alterations of neuronal development may be in part mediated by increased astrocyte GAG levels and neurocan and brevican expression.
33398638	3	28	from	present	365:371	arg1	space					394:398	the extracellular space	376:398	the extracellular space	376:398	Glycosaminoglycans (GAGs) are a family of polysaccharides present in the extracellular space; chondroitin sulfate (CS)- and heparan sulfate (HS)-GAGs are covalently bound to core proteins to form proteoglycans (PGs).
33398638	8	29	theme	whole	1230:1234	arg1	cortex					1247:1252	the whole developing cortex	1226:1252	the whole developing cortex	1226:1252	Cortical astrocytes produce low levels of HA and show low expression of genes involved in HA biosynthesis compared to the whole developing cortex.
33398638	10	30	theme	cortex	1537:1542	arg1	astrocytes					1508:1517	astrocytes	1508:1517	astrocytes of the developing cortex	1508:1542	Ethanol upregulates C4S-GAGs as well as brain-specific lecticans neurocan and brevican, which are highly enriched in astrocytes of the developing cortex in vivo.
33398638	0	31	theme	Rats	120:123	arg1	Characterization					0:15	Characterization	0:15	Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures	0:91	Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures and the Cortex of Neonatal Rats.
33398638	0	31	theme	Rats	120:123	arg1	Cortex					101:106	the Cortex	97:106	the Cortex of Neonatal Rats	97:123	Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures and the Cortex of Neonatal Rats.
33398638	0	32	from	Composition	51:61	arg1	Cultures					84:91	Astrocyte Primary Cultures	66:91	Astrocyte Primary Cultures	66:91	Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures and the Cortex of Neonatal Rats.
33398638	11	33	theme	astrocyte	1759:1767	arg1	levels					1773:1778	increased astrocyte GAG levels	1749:1778	increased astrocyte GAG levels	1749:1778	These results begin to elucidate the role of astrocytes in the biosynthesis of CS- HS- and HA-GAGs, and suggest that ethanol-induced alterations of neuronal development may be in part mediated by increased astrocyte GAG levels and neurocan and brevican expression.
33398638	3	34	theme	present	365:371	arg1	polysaccharides					349:363	polysaccharides	349:363	polysaccharides present in the extracellular space	349:398	Glycosaminoglycans (GAGs) are a family of polysaccharides present in the extracellular space; chondroitin sulfate (CS)- and heparan sulfate (HS)-GAGs are covalently bound to core proteins to form proteoglycans (PGs).
33398638	3	35	theme	sulfate	439:445	arg1	-GAGs					451:455	chondroitin sulfate (CS)- and heparan sulfate (HS)-GAGs	401:455	chondroitin sulfate (CS)- and heparan sulfate (HS)-GAGs	401:455	Glycosaminoglycans (GAGs) are a family of polysaccharides present in the extracellular space; chondroitin sulfate (CS)- and heparan sulfate (HS)-GAGs are covalently bound to core proteins to form proteoglycans (PGs).
33398638	7	36	theme	affinity	1049:1056	arg1	TRAP					1072:1075	TRAP	1072:1075	TRAP	1072:1075	Finally, we investigated the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM, in vivo using translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice.
33398638	7	36	theme	affinity	1049:1056	arg1	purification					1058:1069	translating ribosome affinity purification	1028:1069	translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice	1028:1105	Finally, we investigated the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM, in vivo using translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice.
33398638	3	37	theme	chondroitin	401:411	arg1	CS					422:423	CS	422:423	CS	422:423	Glycosaminoglycans (GAGs) are a family of polysaccharides present in the extracellular space; chondroitin sulfate (CS)- and heparan sulfate (HS)-GAGs are covalently bound to core proteins to form proteoglycans (PGs).
33398638	3	37	theme	chondroitin	401:411	arg1	sulfate					413:419	chondroitin sulfate	401:419	chondroitin sulfate (CS)	401:424	Glycosaminoglycans (GAGs) are a family of polysaccharides present in the extracellular space; chondroitin sulfate (CS)- and heparan sulfate (HS)-GAGs are covalently bound to core proteins to form proteoglycans (PGs).
33398638	11	38	theme	neurocan	1784:1791	arg1	expression					1806:1815	neurocan and brevican expression	1784:1815	neurocan and brevican expression	1784:1815	These results begin to elucidate the role of astrocytes in the biosynthesis of CS- HS- and HA-GAGs, and suggest that ethanol-induced alterations of neuronal development may be in part mediated by increased astrocyte GAG levels and neurocan and brevican expression.
33398638	7	39	theme	translating	1028:1038	arg1	TRAP					1072:1075	TRAP	1072:1075	TRAP	1072:1075	Finally, we investigated the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM, in vivo using translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice.
33398638	7	39	theme	translating	1028:1038	arg1	purification					1058:1069	translating ribosome affinity purification	1028:1069	translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice	1028:1105	Finally, we investigated the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM, in vivo using translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice.
33398638	0	40	theme	Composition	51:61	arg1	Characterization					0:15	Characterization	0:15	Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures	0:91	Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures and the Cortex of Neonatal Rats.
33398638	0	40	theme	Composition	51:61	arg1	Cortex					101:106	the Cortex	97:106	the Cortex of Neonatal Rats	97:123	Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures and the Cortex of Neonatal Rats.
33398638	0	41	theme	Astrocyte	66:74	arg1	Cultures					84:91	Astrocyte Primary Cultures	66:91	Astrocyte Primary Cultures	66:91	Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures and the Cortex of Neonatal Rats.
33398638	6	42	from	levels	848:853	arg1	astrocytes					858:867	astrocytes	858:867	astrocytes	858:867	We also explored alterations induced by ethanol in GAG and core protein levels in astrocytes.
33398638	11	43	theme	astrocytes	1598:1607	arg1	role					1590:1593	the role	1586:1593	the role of astrocytes in the biosynthesis of CS- HS- and HA-GAGs	1586:1650	These results begin to elucidate the role of astrocytes in the biosynthesis of CS- HS- and HA-GAGs, and suggest that ethanol-induced alterations of neuronal development may be in part mediated by increased astrocyte GAG levels and neurocan and brevican expression.
33398638	11	44	theme	development	1710:1720	arg1	alterations					1686:1696	ethanol-induced alterations	1670:1696	ethanol-induced alterations of neuronal development	1670:1720	These results begin to elucidate the role of astrocytes in the biosynthesis of CS- HS- and HA-GAGs, and suggest that ethanol-induced alterations of neuronal development may be in part mediated by increased astrocyte GAG levels and neurocan and brevican expression.
33398638	5	45	theme	astrocyte	728:736	arg1	cultures					738:745	cortical astrocyte cultures	719:745	cortical astrocyte cultures	719:745	In this study we investigated the contribution of astrocytes to CS-, HS-, and HA-GAG production by comparing the makeup of these GAGs in cortical astrocyte cultures and the neonatal rat cortex.
33398638	6	46	theme	GAG	827:829	arg1	levels					848:853	GAG and core protein levels	827:853	GAG and core protein levels in astrocytes	827:867	We also explored alterations induced by ethanol in GAG and core protein levels in astrocytes.
33398638	10	47	dep	upregulates	1399:1409	arg1	enriched					1496:1503	enriched	1496:1503	enriched	1496:1503	Ethanol upregulates C4S-GAGs as well as brain-specific lecticans neurocan and brevican, which are highly enriched in astrocytes of the developing cortex in vivo.
33398638	7	48	theme	ECM	1009:1011	arg1	components					985:994	major components	979:994	major components of the brain ECM	979:1011	Finally, we investigated the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM, in vivo using translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice.
33398638	7	48	theme	ECM	1009:1011	arg1	proteins					969:976	proteins	969:976	proteins	969:976	Finally, we investigated the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM, in vivo using translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice.
33398638	1	49	theme	developing	235:244	arg1	brain					246:250	the developing brain	231:250	the developing brain	231:250	Astrocytes are major producers of the extracellular matrix (ECM), which is involved in the plasticity of the developing brain.
33398638	4	50	theme	Hyaluronic	524:533	arg1	HA					541:542	HA	541:542	HA	541:542	Hyaluronic acid (HA)-GAGs are not bound to core proteins.
33398638	4	50	theme	Hyaluronic	524:533	arg1	acid					535:538	Hyaluronic acid	524:538	Hyaluronic acid (HA)-GAGs	524:548	Hyaluronic acid (HA)-GAGs are not bound to core proteins.
33398638	9	51	theme	C0S	1309:1311	arg1	-GAGs					1313:1317	chondroitin-0-sulfate (C0S)-GAGs	1286:1317	chondroitin-0-sulfate (C0S)-GAGs (possibly because of a higher sulfatase enzyme expression)	1286:1376	Astrocytes have high levels of chondroitin-0-sulfate (C0S)-GAGs (possibly because of a higher sulfatase enzyme expression) and HS-GAGs.
33398638	10	52	from	enriched	1496:1503	arg1	astrocytes					1508:1517	astrocytes	1508:1517	astrocytes of the developing cortex	1508:1542	Ethanol upregulates C4S-GAGs as well as brain-specific lecticans neurocan and brevican, which are highly enriched in astrocytes of the developing cortex in vivo.
33398638	9	53	contain	have	1266:1269	arg2	levels					1276:1281	high levels	1271:1281	high levels of chondroitin-0-sulfate (C0S)-GAGs (possibly because of a higher sulfatase enzyme expression) and HS-GAGs	1271:1388	Astrocytes have high levels of chondroitin-0-sulfate (C0S)-GAGs (possibly because of a higher sulfatase enzyme expression) and HS-GAGs.
33398638	9	53	contain	have	1266:1269	arg1	Astrocytes					1255:1264	Astrocytes	1255:1264	Astrocytes	1255:1264	Astrocytes have high levels of chondroitin-0-sulfate (C0S)-GAGs (possibly because of a higher sulfatase enzyme expression) and HS-GAGs.
33398638	2	54	theme	alcohol	262:268	arg1	exposure					270:277	In utero alcohol exposure	253:277	In utero alcohol exposure	253:277	In utero alcohol exposure alters neuronal plasticity.
33398638	5	55	theme	rat	764:766	arg1	cortex					768:773	the neonatal rat cortex	751:773	the neonatal rat cortex	751:773	In this study we investigated the contribution of astrocytes to CS-, HS-, and HA-GAG production by comparing the makeup of these GAGs in cortical astrocyte cultures and the neonatal rat cortex.
33398638	10	56	from	astrocytes	1508:1517	arg1	enriched					1496:1503	enriched	1496:1503	enriched	1496:1503	Ethanol upregulates C4S-GAGs as well as brain-specific lecticans neurocan and brevican, which are highly enriched in astrocytes of the developing cortex in vivo.
33398638	8	57	theme	low	1136:1138	arg1	levels					1140:1145	low levels	1136:1145	low levels of HA	1136:1151	Cortical astrocytes produce low levels of HA and show low expression of genes involved in HA biosynthesis compared to the whole developing cortex.
33398638	10	58	dep	lecticans	1446:1454	arg1	lecticans					1446:1454	brain-specific lecticans	1431:1454	C4S-GAGs as well as brain-specific lecticans neurocan and brevican	1411:1476	Ethanol upregulates C4S-GAGs as well as brain-specific lecticans neurocan and brevican, which are highly enriched in astrocytes of the developing cortex in vivo.
33398638	10	58	dep	lecticans	1446:1454	arg1	brevican					1469:1476	brevican	1469:1476	brevican	1469:1476	Ethanol upregulates C4S-GAGs as well as brain-specific lecticans neurocan and brevican, which are highly enriched in astrocytes of the developing cortex in vivo.
33398638	10	58	dep	lecticans	1446:1454	arg1	neurocan					1456:1463	neurocan	1456:1463	neurocan	1456:1463	Ethanol upregulates C4S-GAGs as well as brain-specific lecticans neurocan and brevican, which are highly enriched in astrocytes of the developing cortex in vivo.
33398638	0	59	from	Cortex	101:106	arg1	Cultures					84:91	Astrocyte Primary Cultures	66:91	Astrocyte Primary Cultures	66:91	Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures and the Cortex of Neonatal Rats.
33398638	7	60	from	expression	908:917	arg1	astrocytes					922:931	astrocytes	922:931	astrocytes	922:931	Finally, we investigated the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM, in vivo using translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice.
33398638	7	61	theme	lectican	950:957	arg1	family					959:964	the lectican family	946:964	the lectican family of proteins, major components of the brain ECM,	946:1012	Finally, we investigated the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM, in vivo using translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice.
33398638	9	62	theme	higher	1342:1347	arg1	expression					1366:1375	a higher sulfatase enzyme expression	1340:1375	a higher sulfatase enzyme expression	1340:1375	Astrocytes have high levels of chondroitin-0-sulfate (C0S)-GAGs (possibly because of a higher sulfatase enzyme expression) and HS-GAGs.
33398638	4	63	theme	core	567:570	arg1	proteins					572:579	core proteins	567:579	core proteins	567:579	Hyaluronic acid (HA)-GAGs are not bound to core proteins.
33398638	11	64	theme	neuronal	1701:1708	arg1	development					1710:1720	neuronal development	1701:1720	neuronal development	1701:1720	These results begin to elucidate the role of astrocytes in the biosynthesis of CS- HS- and HA-GAGs, and suggest that ethanol-induced alterations of neuronal development may be in part mediated by increased astrocyte GAG levels and neurocan and brevican expression.
33398638	9	65	theme	enzyme	1359:1364	arg1	expression					1366:1375	a higher sulfatase enzyme expression	1340:1375	a higher sulfatase enzyme expression	1340:1375	Astrocytes have high levels of chondroitin-0-sulfate (C0S)-GAGs (possibly because of a higher sulfatase enzyme expression) and HS-GAGs.
33398638	6	66	theme	core	835:838	arg1	protein					840:846	core protein	835:846	core protein	835:846	We also explored alterations induced by ethanol in GAG and core protein levels in astrocytes.
33398638	8	67	theme	low	1162:1164	arg1	expression					1166:1175	low expression	1162:1175	low expression of genes involved in HA biosynthesis compared to the whole developing cortex	1162:1252	Cortical astrocytes produce low levels of HA and show low expression of genes involved in HA biosynthesis compared to the whole developing cortex.
33398638	7	68	theme	ribosome	1040:1047	arg1	TRAP					1072:1075	TRAP	1072:1075	TRAP	1072:1075	Finally, we investigated the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM, in vivo using translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice.
33398638	7	68	theme	ribosome	1040:1047	arg1	purification					1058:1069	translating ribosome affinity purification	1028:1069	translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice	1028:1105	Finally, we investigated the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM, in vivo using translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice.
33398638	2	69	theme	In	253:254	arg1	exposure					270:277	In utero alcohol exposure	253:277	In utero alcohol exposure	253:277	In utero alcohol exposure alters neuronal plasticity.
33398638	1	70	theme	major	141:145	arg1	Astrocytes					126:135	Astrocytes	126:135	Astrocytes	126:135	Astrocytes are major producers of the extracellular matrix (ECM), which is involved in the plasticity of the developing brain.
33398638	1	70	theme	major	141:145	arg1	producers					147:155	major producers	141:155	major producers of the extracellular matrix (ECM), which is involved in the plasticity of the developing brain	141:250	Astrocytes are major producers of the extracellular matrix (ECM), which is involved in the plasticity of the developing brain.
33398638	5	71	theme	HA-GAG	660:665	arg1	production					667:676	HA-GAG production	660:676	HA-GAG production	660:676	In this study we investigated the contribution of astrocytes to CS-, HS-, and HA-GAG production by comparing the makeup of these GAGs in cortical astrocyte cultures and the neonatal rat cortex.
33398638	9	72	theme	HS-GAGs	1382:1388	arg1	levels					1276:1281	high levels	1271:1281	high levels of chondroitin-0-sulfate (C0S)-GAGs (possibly because of a higher sulfatase enzyme expression) and HS-GAGs	1271:1388	Astrocytes have high levels of chondroitin-0-sulfate (C0S)-GAGs (possibly because of a higher sulfatase enzyme expression) and HS-GAGs.
33398638	11	73	from	role	1590:1593	arg1	biosynthesis					1616:1627	the biosynthesis	1612:1627	the biosynthesis of CS- HS- and HA-GAGs	1612:1650	These results begin to elucidate the role of astrocytes in the biosynthesis of CS- HS- and HA-GAGs, and suggest that ethanol-induced alterations of neuronal development may be in part mediated by increased astrocyte GAG levels and neurocan and brevican expression.
33398638	7	74	theme	Aldh1l1-EGFP-Rpl10a	1082:1100	arg1	mice					1102:1105	Aldh1l1-EGFP-Rpl10a mice	1082:1105	Aldh1l1-EGFP-Rpl10a mice	1082:1105	Finally, we investigated the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM, in vivo using translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice.
33398638	5	75	from	cortex	768:773	arg1	cultures					738:745	cortical astrocyte cultures	719:745	cortical astrocyte cultures	719:745	In this study we investigated the contribution of astrocytes to CS-, HS-, and HA-GAG production by comparing the makeup of these GAGs in cortical astrocyte cultures and the neonatal rat cortex.
33398638	1	76	theme	extracellular	164:176	arg1	ECM					186:188	ECM	186:188	ECM	186:188	Astrocytes are major producers of the extracellular matrix (ECM), which is involved in the plasticity of the developing brain.
33398638	1	76	theme	extracellular	164:176	arg1	matrix					178:183	extracellular matrix	164:183	the extracellular matrix (ECM)	160:189	Astrocytes are major producers of the extracellular matrix (ECM), which is involved in the plasticity of the developing brain.
33398638	5	77	theme	astrocytes	632:641	arg1	contribution					616:627	the contribution	612:627	the contribution of astrocytes to CS-, HS-, and HA-GAG production	612:676	In this study we investigated the contribution of astrocytes to CS-, HS-, and HA-GAG production by comparing the makeup of these GAGs in cortical astrocyte cultures and the neonatal rat cortex.
33398638	8	78	theme	HA	1198:1199	arg1	biosynthesis					1201:1212	HA biosynthesis	1198:1212	HA biosynthesis	1198:1212	Cortical astrocytes produce low levels of HA and show low expression of genes involved in HA biosynthesis compared to the whole developing cortex.
33398638	11	79	theme	HA-GAGs	1644:1650	arg1	biosynthesis					1616:1627	the biosynthesis	1612:1627	the biosynthesis of CS- HS- and HA-GAGs	1612:1650	These results begin to elucidate the role of astrocytes in the biosynthesis of CS- HS- and HA-GAGs, and suggest that ethanol-induced alterations of neuronal development may be in part mediated by increased astrocyte GAG levels and neurocan and brevican expression.
33398638	0	80	theme	Neonatal	111:118	arg1	Rats					120:123	Neonatal Rats	111:123	Neonatal Rats	111:123	Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures and the Cortex of Neonatal Rats.
33398638	5	81	from	makeup	695:700	arg1	cultures					738:745	cortical astrocyte cultures	719:745	cortical astrocyte cultures	719:745	In this study we investigated the contribution of astrocytes to CS-, HS-, and HA-GAG production by comparing the makeup of these GAGs in cortical astrocyte cultures and the neonatal rat cortex.
33398638	3	82	theme	polysaccharides	349:363	arg1	family					339:344	a family	337:344	a family of polysaccharides present in the extracellular space	337:398	Glycosaminoglycans (GAGs) are a family of polysaccharides present in the extracellular space; chondroitin sulfate (CS)- and heparan sulfate (HS)-GAGs are covalently bound to core proteins to form proteoglycans (PGs).
33398638	3	82	theme	polysaccharides	349:363	arg1	Glycosaminoglycans					307:324	Glycosaminoglycans	307:324	Glycosaminoglycans (GAGs)	307:331	Glycosaminoglycans (GAGs) are a family of polysaccharides present in the extracellular space; chondroitin sulfate (CS)- and heparan sulfate (HS)-GAGs are covalently bound to core proteins to form proteoglycans (PGs).
33398638	11	83	theme	HS-	1636:1638	arg1	biosynthesis					1616:1627	the biosynthesis	1612:1627	the biosynthesis of CS- HS- and HA-GAGs	1612:1650	These results begin to elucidate the role of astrocytes in the biosynthesis of CS- HS- and HA-GAGs, and suggest that ethanol-induced alterations of neuronal development may be in part mediated by increased astrocyte GAG levels and neurocan and brevican expression.
33398638	7	84	from	purification	1058:1069	arg1	mice					1102:1105	Aldh1l1-EGFP-Rpl10a mice	1082:1105	Aldh1l1-EGFP-Rpl10a mice	1082:1105	Finally, we investigated the relative expression in astrocytes of CS-PGs of the lectican family of proteins, major components of the brain ECM, in vivo using translating ribosome affinity purification (TRAP) (in Aldh1l1-EGFP-Rpl10a mice.
33398638	10	85	theme	developing	1526:1535	arg1	cortex					1537:1542	the developing cortex	1522:1542	the developing cortex	1522:1542	Ethanol upregulates C4S-GAGs as well as brain-specific lecticans neurocan and brevican, which are highly enriched in astrocytes of the developing cortex in vivo.
33398638	5	86	theme	GAGs	711:714	arg1	cortex					768:773	the neonatal rat cortex	751:773	the neonatal rat cortex	751:773	In this study we investigated the contribution of astrocytes to CS-, HS-, and HA-GAG production by comparing the makeup of these GAGs in cortical astrocyte cultures and the neonatal rat cortex.
33398638	5	86	theme	GAGs	711:714	arg1	makeup					695:700	the makeup	691:700	the makeup of these GAGs in cortical astrocyte cultures	691:745	In this study we investigated the contribution of astrocytes to CS-, HS-, and HA-GAG production by comparing the makeup of these GAGs in cortical astrocyte cultures and the neonatal rat cortex.
33398638	3	87	theme	extracellular	380:392	arg1	space					394:398	the extracellular space	376:398	the extracellular space	376:398	Glycosaminoglycans (GAGs) are a family of polysaccharides present in the extracellular space; chondroitin sulfate (CS)- and heparan sulfate (HS)-GAGs are covalently bound to core proteins to form proteoglycans (PGs).
33398638	8	88	theme	developing	1236:1245	arg1	cortex					1247:1252	the whole developing cortex	1226:1252	the whole developing cortex	1226:1252	Cortical astrocytes produce low levels of HA and show low expression of genes involved in HA biosynthesis compared to the whole developing cortex.
33398638	11	89	theme	GAG	1769:1771	arg1	levels					1773:1778	increased astrocyte GAG levels	1749:1778	increased astrocyte GAG levels	1749:1778	These results begin to elucidate the role of astrocytes in the biosynthesis of CS- HS- and HA-GAGs, and suggest that ethanol-induced alterations of neuronal development may be in part mediated by increased astrocyte GAG levels and neurocan and brevican expression.
33398638	0	90	theme	Disaccharide	38:49	arg1	Composition					51:61	Glycosaminoglycan Disaccharide Composition	20:61	Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures	20:91	Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures and the Cortex of Neonatal Rats.
33398638	3	91	theme	sulfate	413:419	arg1	-GAGs					451:455	chondroitin sulfate (CS)- and heparan sulfate (HS)-GAGs	401:455	chondroitin sulfate (CS)- and heparan sulfate (HS)-GAGs	401:455	Glycosaminoglycans (GAGs) are a family of polysaccharides present in the extracellular space; chondroitin sulfate (CS)- and heparan sulfate (HS)-GAGs are covalently bound to core proteins to form proteoglycans (PGs).
33398638	3	92	theme	heparan	431:437	arg1	HS					448:449	HS	448:449	HS	448:449	Glycosaminoglycans (GAGs) are a family of polysaccharides present in the extracellular space; chondroitin sulfate (CS)- and heparan sulfate (HS)-GAGs are covalently bound to core proteins to form proteoglycans (PGs).
33398638	3	92	theme	heparan	431:437	arg1	sulfate					439:445	heparan sulfate	431:445	heparan sulfate (HS)	431:450	Glycosaminoglycans (GAGs) are a family of polysaccharides present in the extracellular space; chondroitin sulfate (CS)- and heparan sulfate (HS)-GAGs are covalently bound to core proteins to form proteoglycans (PGs).
33398638	0	93	theme	Primary	76:82	arg1	Cultures					84:91	Astrocyte Primary Cultures	66:91	Astrocyte Primary Cultures	66:91	Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures and the Cortex of Neonatal Rats.
33398638	11	94	theme	increased	1749:1757	arg1	levels					1773:1778	increased astrocyte GAG levels	1749:1778	increased astrocyte GAG levels	1749:1778	These results begin to elucidate the role of astrocytes in the biosynthesis of CS- HS- and HA-GAGs, and suggest that ethanol-induced alterations of neuronal development may be in part mediated by increased astrocyte GAG levels and neurocan and brevican expression.
33398638	9	95	theme	high	1271:1274	arg1	levels					1276:1281	high levels	1271:1281	high levels of chondroitin-0-sulfate (C0S)-GAGs (possibly because of a higher sulfatase enzyme expression) and HS-GAGs	1271:1388	Astrocytes have high levels of chondroitin-0-sulfate (C0S)-GAGs (possibly because of a higher sulfatase enzyme expression) and HS-GAGs.
33398638	5	96	theme	neonatal	755:762	arg1	cortex					768:773	the neonatal rat cortex	751:773	the neonatal rat cortex	751:773	In this study we investigated the contribution of astrocytes to CS-, HS-, and HA-GAG production by comparing the makeup of these GAGs in cortical astrocyte cultures and the neonatal rat cortex.
33398638	3	97	theme	core	481:484	arg1	proteins					486:493	core proteins	481:493	core proteins	481:493	Glycosaminoglycans (GAGs) are a family of polysaccharides present in the extracellular space; chondroitin sulfate (CS)- and heparan sulfate (HS)-GAGs are covalently bound to core proteins to form proteoglycans (PGs).
32933702	4	0	theme	Structural	522:531	arg1	features					533:540	Structural features	522:540	Structural features of LFP-1	522:549	Structural features of LFP-1 were analyzed with molecular weight (MW) distribution, monosaccharide composition, methylation and nuclear magnetic resonance (NMR) spectra.
32933702	7	1	theme	model	1101:1105	arg1	cells					1112:1116	PD model PC12 cells	1098:1116	PD model PC12 cells	1098:1116	Furthermore, LFP-1 had a significantly protective effect against 1-methyl-4-phenylpyridiniumion (MPP+)-induced neurotoxicity in PD model PC12 cells.
32933702	8	2	from	neurodegeneration	1274:1290	arg1	PD					1295:1296	PD	1295:1296	PD	1295:1296	These observations unambiguously indicated the neurotrophic and neuroprotective activities of LFP-1, which may be developed for prevention or treatment of neurodegeneration in PD.
32933702	4	3	theme	LFP-1	545:549	arg1	features					533:540	Structural features	522:540	Structural features of LFP-1	522:549	Structural features of LFP-1 were analyzed with molecular weight (MW) distribution, monosaccharide composition, methylation and nuclear magnetic resonance (NMR) spectra.
32933702	7	4	theme	protective	1009:1018	arg1	effect					1020:1025	a significantly protective effect	993:1025	a significantly protective effect against 1-methyl-4-phenylpyridiniumion (MPP+)-induced neurotoxicity in PD model PC12 cells	993:1116	Furthermore, LFP-1 had a significantly protective effect against 1-methyl-4-phenylpyridiniumion (MPP+)-induced neurotoxicity in PD model PC12 cells.
32933702	7	5	from	effect	1020:1025	arg1	cells					1112:1116	PD model PC12 cells	1098:1116	PD model PC12 cells	1098:1116	Furthermore, LFP-1 had a significantly protective effect against 1-methyl-4-phenylpyridiniumion (MPP+)-induced neurotoxicity in PD model PC12 cells.
32933702	0	6	theme	neuroprotective	100:114	arg1	activities					116:125	neuroprotective activities	100:125	neuroprotective activities	100:125	An acidic heteropolysaccharide from Lycii fructus: Purification, characterization, neurotrophic and neuroprotective activities in vitro.
32933702	6	7	theme	PC12	952:955	arg1	models					962:967	PC12 cell models	952:967	PC12 cell models	952:967	LFP-1 promoted neuronal differentiation and neurite outgrowth in vitro in PC12 cell models.
32933702	0	8	dep	in	127:128	arg1	vitro					130:134	vitro	130:134	vitro	130:134	An acidic heteropolysaccharide from Lycii fructus: Purification, characterization, neurotrophic and neuroprotective activities in vitro.
32933702	8	9	from	treatment	1261:1269	arg1	PD					1295:1296	PD	1295:1296	PD	1295:1296	These observations unambiguously indicated the neurotrophic and neuroprotective activities of LFP-1, which may be developed for prevention or treatment of neurodegeneration in PD.
32933702	3	10	theme	filtration	495:504	arg1	chromatography					506:519	gel filtration chromatography	491:519	gel filtration chromatography	491:519	In this study, an acidic heteropolysaccharide LFP-1 was isolated from Lycii fructus, and purified by ion-exchange and gel filtration chromatography.
32933702	0	11	theme	in	127:128	arg1	Purification					51:62	Purification	51:62	Purification	51:62	An acidic heteropolysaccharide from Lycii fructus: Purification, characterization, neurotrophic and neuroprotective activities in vitro.
32933702	2	12	theme	potential	290:298	arg1	application					300:310	the potential application	286:310	the potential application of natural polysaccharides in prevention or treatment of PD	286:370	Increasing evidence is supporting the potential application of natural polysaccharides in prevention or treatment of PD.
32933702	8	13	theme	neurodegeneration	1274:1290	arg1	treatment					1261:1269	treatment	1261:1269	treatment	1261:1269	These observations unambiguously indicated the neurotrophic and neuroprotective activities of LFP-1, which may be developed for prevention or treatment of neurodegeneration in PD.
32933702	8	13	theme	neurodegeneration	1274:1290	arg1	prevention					1247:1256	prevention	1247:1256	prevention	1247:1256	These observations unambiguously indicated the neurotrophic and neuroprotective activities of LFP-1, which may be developed for prevention or treatment of neurodegeneration in PD.
32933702	1	14	theme	neurotrophic	184:195	arg1	strategy					213:220	a neurotrophic and therapeutic strategy	182:220	a neurotrophic and therapeutic strategy for Parkinson's disease (PD)	182:249	Regeneration of neurites network constitutes a neurotrophic and therapeutic strategy for Parkinson's disease (PD).
32933702	4	15	theme	monosaccharide	606:619	arg1	composition					621:631	monosaccharide composition	606:631	monosaccharide composition	606:631	Structural features of LFP-1 were analyzed with molecular weight (MW) distribution, monosaccharide composition, methylation and nuclear magnetic resonance (NMR) spectra.
32933702	4	15	theme	monosaccharide	606:619	arg1	weight					580:585	molecular weight	570:585	molecular weight	570:585	Structural features of LFP-1 were analyzed with molecular weight (MW) distribution, monosaccharide composition, methylation and nuclear magnetic resonance (NMR) spectra.
32933702	0	16	from	fructus	42:48	arg1	heteropolysaccharide					10:29	An acidic heteropolysaccharide	0:29	An acidic heteropolysaccharide from Lycii fructus: Purification, characterization, neurotrophic and neuroprotective activities in vitro.	0:135	An acidic heteropolysaccharide from Lycii fructus: Purification, characterization, neurotrophic and neuroprotective activities in vitro.
32933702	5	17	theme	1.78	764:767	arg1	×					769:769	×	769:769	×	769:769	LFP-1 was a complicated structured polysaccharide with an average MW of 1.78 × 104 Da and composed of highly branched arabinogalactans, homogalacturonan and rhamnogalacturonan moieties.
32933702	0	18	theme	acidic	3:8	arg1	heteropolysaccharide					10:29	An acidic heteropolysaccharide	0:29	An acidic heteropolysaccharide from Lycii fructus: Purification, characterization, neurotrophic and neuroprotective activities in vitro.	0:135	An acidic heteropolysaccharide from Lycii fructus: Purification, characterization, neurotrophic and neuroprotective activities in vitro.
32933702	4	19	dep	weight	580:585	arg1	distribution					592:603	distribution	592:603	distribution	592:603	Structural features of LFP-1 were analyzed with molecular weight (MW) distribution, monosaccharide composition, methylation and nuclear magnetic resonance (NMR) spectra.
32933702	1	20	theme	therapeutic	201:211	arg1	strategy					213:220	a neurotrophic and therapeutic strategy	182:220	a neurotrophic and therapeutic strategy for Parkinson's disease (PD)	182:249	Regeneration of neurites network constitutes a neurotrophic and therapeutic strategy for Parkinson's disease (PD).
32933702	3	21	theme	heteropolysaccharide	398:417	arg1	LFP-1					419:423	an acidic heteropolysaccharide LFP-1	388:423	an acidic heteropolysaccharide LFP-1	388:423	In this study, an acidic heteropolysaccharide LFP-1 was isolated from Lycii fructus, and purified by ion-exchange and gel filtration chromatography.
32933702	7	22	contain	had	989:991	arg1	LFP-1					983:987	LFP-1	983:987	LFP-1	983:987	Furthermore, LFP-1 had a significantly protective effect against 1-methyl-4-phenylpyridiniumion (MPP+)-induced neurotoxicity in PD model PC12 cells.
32933702	7	22	contain	had	989:991	arg2	effect					1020:1025	a significantly protective effect	993:1025	a significantly protective effect against 1-methyl-4-phenylpyridiniumion (MPP+)-induced neurotoxicity in PD model PC12 cells	993:1116	Furthermore, LFP-1 had a significantly protective effect against 1-methyl-4-phenylpyridiniumion (MPP+)-induced neurotoxicity in PD model PC12 cells.
32933702	2	23	theme	polysaccharides	323:337	arg1	application					300:310	the potential application	286:310	the potential application of natural polysaccharides in prevention or treatment of PD	286:370	Increasing evidence is supporting the potential application of natural polysaccharides in prevention or treatment of PD.
32933702	4	24	theme	resonance	667:675	arg1	spectra					683:689	nuclear magnetic resonance (NMR) spectra	650:689	nuclear magnetic resonance (NMR) spectra	650:689	Structural features of LFP-1 were analyzed with molecular weight (MW) distribution, monosaccharide composition, methylation and nuclear magnetic resonance (NMR) spectra.
32933702	5	25	theme	rhamnogalacturonan	849:866	arg1	moieties					868:875	rhamnogalacturonan moieties	849:875	rhamnogalacturonan moieties	849:875	LFP-1 was a complicated structured polysaccharide with an average MW of 1.78 × 104 Da and composed of highly branched arabinogalactans, homogalacturonan and rhamnogalacturonan moieties.
32933702	5	25	theme	rhamnogalacturonan	849:866	arg1	arabinogalactans					810:825	highly branched arabinogalactans	794:825	highly branched arabinogalactans	794:825	LFP-1 was a complicated structured polysaccharide with an average MW of 1.78 × 104 Da and composed of highly branched arabinogalactans, homogalacturonan and rhamnogalacturonan moieties.
32933702	6	26	theme	neurite	922:928	arg1	outgrowth					930:938	neurite outgrowth	922:938	neurite outgrowth	922:938	LFP-1 promoted neuronal differentiation and neurite outgrowth in vitro in PC12 cell models.
32933702	2	27	theme	natural	315:321	arg1	polysaccharides					323:337	natural polysaccharides	315:337	natural polysaccharides	315:337	Increasing evidence is supporting the potential application of natural polysaccharides in prevention or treatment of PD.
32933702	4	28	theme	magnetic	658:665	arg1	NMR					678:680	NMR	678:680	NMR	678:680	Structural features of LFP-1 were analyzed with molecular weight (MW) distribution, monosaccharide composition, methylation and nuclear magnetic resonance (NMR) spectra.
32933702	4	28	theme	magnetic	658:665	arg1	resonance					667:675	nuclear magnetic resonance	650:675	nuclear magnetic resonance (NMR) spectra	650:689	Structural features of LFP-1 were analyzed with molecular weight (MW) distribution, monosaccharide composition, methylation and nuclear magnetic resonance (NMR) spectra.
32933702	3	29	attach	isolated	429:436	arg2	LFP-1					419:423	an acidic heteropolysaccharide LFP-1	388:423	an acidic heteropolysaccharide LFP-1	388:423	In this study, an acidic heteropolysaccharide LFP-1 was isolated from Lycii fructus, and purified by ion-exchange and gel filtration chromatography.
32933702	3	29	attach	isolated	429:436	arg1	fructus					449:455	Lycii fructus	443:455	Lycii fructus	443:455	In this study, an acidic heteropolysaccharide LFP-1 was isolated from Lycii fructus, and purified by ion-exchange and gel filtration chromatography.
32933702	5	30	with	polysaccharide	727:740	arg1	MW					758:759	an average MW	747:759	an average MW of 1.78 × 104 Da	747:776	LFP-1 was a complicated structured polysaccharide with an average MW of 1.78 × 104 Da and composed of highly branched arabinogalactans, homogalacturonan and rhamnogalacturonan moieties.
32933702	0	31	theme	Lycii	36:40	arg1	fructus					42:48	Lycii fructus	36:48	Lycii fructus	36:48	An acidic heteropolysaccharide from Lycii fructus: Purification, characterization, neurotrophic and neuroprotective activities in vitro.
32933702	5	32	theme	average	750:756	arg1	MW					758:759	an average MW	747:759	an average MW of 1.78 × 104 Da	747:776	LFP-1 was a complicated structured polysaccharide with an average MW of 1.78 × 104 Da and composed of highly branched arabinogalactans, homogalacturonan and rhamnogalacturonan moieties.
32933702	5	33	theme	branched	801:808	arg1	homogalacturonan					828:843	homogalacturonan	828:843	homogalacturonan	828:843	LFP-1 was a complicated structured polysaccharide with an average MW of 1.78 × 104 Da and composed of highly branched arabinogalactans, homogalacturonan and rhamnogalacturonan moieties.
32933702	5	33	theme	branched	801:808	arg1	moieties					868:875	rhamnogalacturonan moieties	849:875	rhamnogalacturonan moieties	849:875	LFP-1 was a complicated structured polysaccharide with an average MW of 1.78 × 104 Da and composed of highly branched arabinogalactans, homogalacturonan and rhamnogalacturonan moieties.
32933702	5	33	theme	branched	801:808	arg1	arabinogalactans					810:825	highly branched arabinogalactans	794:825	highly branched arabinogalactans	794:825	LFP-1 was a complicated structured polysaccharide with an average MW of 1.78 × 104 Da and composed of highly branched arabinogalactans, homogalacturonan and rhamnogalacturonan moieties.
32933702	7	34	theme	PC12	1107:1110	arg1	cells					1112:1116	PD model PC12 cells	1098:1116	PD model PC12 cells	1098:1116	Furthermore, LFP-1 had a significantly protective effect against 1-methyl-4-phenylpyridiniumion (MPP+)-induced neurotoxicity in PD model PC12 cells.
32933702	6	35	theme	neuronal	893:900	arg1	differentiation					902:916	neuronal differentiation	893:916	neuronal differentiation	893:916	LFP-1 promoted neuronal differentiation and neurite outgrowth in vitro in PC12 cell models.
32933702	7	36	theme	PD	1098:1099	arg1	cells					1112:1116	PD model PC12 cells	1098:1116	PD model PC12 cells	1098:1116	Furthermore, LFP-1 had a significantly protective effect against 1-methyl-4-phenylpyridiniumion (MPP+)-induced neurotoxicity in PD model PC12 cells.
32933702	2	37	theme	PD	369:370	arg1	treatment					356:364	treatment	356:364	treatment	356:364	Increasing evidence is supporting the potential application of natural polysaccharides in prevention or treatment of PD.
32933702	2	37	theme	PD	369:370	arg1	prevention					342:351	prevention	342:351	prevention	342:351	Increasing evidence is supporting the potential application of natural polysaccharides in prevention or treatment of PD.
32933702	8	38	theme	neurotrophic	1166:1177	arg1	activities					1199:1208	the neurotrophic and neuroprotective activities	1162:1208	the neurotrophic and neuroprotective activities of LFP-1, which may be developed for prevention or treatment of neurodegeneration in PD	1162:1296	These observations unambiguously indicated the neurotrophic and neuroprotective activities of LFP-1, which may be developed for prevention or treatment of neurodegeneration in PD.
32933702	4	39	theme	molecular	570:578	arg1	methylation					634:644	methylation	634:644	methylation	634:644	Structural features of LFP-1 were analyzed with molecular weight (MW) distribution, monosaccharide composition, methylation and nuclear magnetic resonance (NMR) spectra.
32933702	4	39	theme	molecular	570:578	arg1	composition					621:631	monosaccharide composition	606:631	monosaccharide composition	606:631	Structural features of LFP-1 were analyzed with molecular weight (MW) distribution, monosaccharide composition, methylation and nuclear magnetic resonance (NMR) spectra.
32933702	4	39	theme	molecular	570:578	arg1	MW					588:589	MW	588:589	MW	588:589	Structural features of LFP-1 were analyzed with molecular weight (MW) distribution, monosaccharide composition, methylation and nuclear magnetic resonance (NMR) spectra.
32933702	4	39	theme	molecular	570:578	arg1	weight					580:585	molecular weight	570:585	molecular weight	570:585	Structural features of LFP-1 were analyzed with molecular weight (MW) distribution, monosaccharide composition, methylation and nuclear magnetic resonance (NMR) spectra.
32933702	8	40	theme	neuroprotective	1183:1197	arg1	activities					1199:1208	the neurotrophic and neuroprotective activities	1162:1208	the neurotrophic and neuroprotective activities of LFP-1, which may be developed for prevention or treatment of neurodegeneration in PD	1162:1296	These observations unambiguously indicated the neurotrophic and neuroprotective activities of LFP-1, which may be developed for prevention or treatment of neurodegeneration in PD.
32933702	6	41	theme	cell	957:960	arg1	models					962:967	PC12 cell models	952:967	PC12 cell models	952:967	LFP-1 promoted neuronal differentiation and neurite outgrowth in vitro in PC12 cell models.
32933702	8	42	from	PD	1295:1296	arg1	treatment					1261:1269	treatment	1261:1269	treatment	1261:1269	These observations unambiguously indicated the neurotrophic and neuroprotective activities of LFP-1, which may be developed for prevention or treatment of neurodegeneration in PD.
32933702	8	42	from	PD	1295:1296	arg1	prevention					1247:1256	prevention	1247:1256	prevention	1247:1256	These observations unambiguously indicated the neurotrophic and neuroprotective activities of LFP-1, which may be developed for prevention or treatment of neurodegeneration in PD.
32933702	5	43	theme	complicated	704:714	arg1	polysaccharide					727:740	a complicated structured polysaccharide	702:740	a complicated structured polysaccharide with an average MW of 1.78 × 104 Da	702:776	LFP-1 was a complicated structured polysaccharide with an average MW of 1.78 × 104 Da and composed of highly branched arabinogalactans, homogalacturonan and rhamnogalacturonan moieties.
32933702	5	43	theme	complicated	704:714	arg1	LFP-1					692:696	LFP-1	692:696	LFP-1	692:696	LFP-1 was a complicated structured polysaccharide with an average MW of 1.78 × 104 Da and composed of highly branched arabinogalactans, homogalacturonan and rhamnogalacturonan moieties.
32933702	8	44	from	prevention	1247:1256	arg1	PD					1295:1296	PD	1295:1296	PD	1295:1296	These observations unambiguously indicated the neurotrophic and neuroprotective activities of LFP-1, which may be developed for prevention or treatment of neurodegeneration in PD.
32933702	7	45	theme	-induced	1072:1079	arg1	neurotoxicity					1081:1093	1-methyl-4-phenylpyridiniumion (MPP+)-induced neurotoxicity	1035:1093	1-methyl-4-phenylpyridiniumion (MPP+)-induced neurotoxicity	1035:1093	Furthermore, LFP-1 had a significantly protective effect against 1-methyl-4-phenylpyridiniumion (MPP+)-induced neurotoxicity in PD model PC12 cells.
32933702	3	46	theme	gel	491:493	arg1	chromatography					506:519	gel filtration chromatography	491:519	gel filtration chromatography	491:519	In this study, an acidic heteropolysaccharide LFP-1 was isolated from Lycii fructus, and purified by ion-exchange and gel filtration chromatography.
32933702	5	47	theme	structured	716:725	arg1	polysaccharide					727:740	a complicated structured polysaccharide	702:740	a complicated structured polysaccharide with an average MW of 1.78 × 104 Da	702:776	LFP-1 was a complicated structured polysaccharide with an average MW of 1.78 × 104 Da and composed of highly branched arabinogalactans, homogalacturonan and rhamnogalacturonan moieties.
32933702	5	47	theme	structured	716:725	arg1	LFP-1					692:696	LFP-1	692:696	LFP-1	692:696	LFP-1 was a complicated structured polysaccharide with an average MW of 1.78 × 104 Da and composed of highly branched arabinogalactans, homogalacturonan and rhamnogalacturonan moieties.
32933702	0	48	dep	heteropolysaccharide	10:29	arg1	neurotrophic					83:94	neurotrophic	83:94	neurotrophic	83:94	An acidic heteropolysaccharide from Lycii fructus: Purification, characterization, neurotrophic and neuroprotective activities in vitro.
32933702	0	48	dep	heteropolysaccharide	10:29	arg1	activities					116:125	neuroprotective activities	100:125	neuroprotective activities	100:125	An acidic heteropolysaccharide from Lycii fructus: Purification, characterization, neurotrophic and neuroprotective activities in vitro.
32933702	0	48	dep	heteropolysaccharide	10:29	arg1	characterization					65:80	characterization	65:80	characterization	65:80	An acidic heteropolysaccharide from Lycii fructus: Purification, characterization, neurotrophic and neuroprotective activities in vitro.
32933702	0	48	dep	heteropolysaccharide	10:29	arg1	Purification					51:62	Purification	51:62	Purification	51:62	An acidic heteropolysaccharide from Lycii fructus: Purification, characterization, neurotrophic and neuroprotective activities in vitro.
32933702	8	49	theme	LFP-1	1213:1217	arg1	activities					1199:1208	the neurotrophic and neuroprotective activities	1162:1208	the neurotrophic and neuroprotective activities of LFP-1, which may be developed for prevention or treatment of neurodegeneration in PD	1162:1296	These observations unambiguously indicated the neurotrophic and neuroprotective activities of LFP-1, which may be developed for prevention or treatment of neurodegeneration in PD.
32933702	1	50	theme	network	162:168	arg1	Regeneration					137:148	Regeneration	137:148	Regeneration of neurites network	137:168	Regeneration of neurites network constitutes a neurotrophic and therapeutic strategy for Parkinson's disease (PD).
32933702	5	51	theme	104	771:773	arg1	×					769:769	×	769:769	×	769:769	LFP-1 was a complicated structured polysaccharide with an average MW of 1.78 × 104 Da and composed of highly branched arabinogalactans, homogalacturonan and rhamnogalacturonan moieties.
32933702	1	52	theme	neurites	153:160	arg1	network					162:168	neurites network	153:168	neurites network	153:168	Regeneration of neurites network constitutes a neurotrophic and therapeutic strategy for Parkinson's disease (PD).
32933702	2	53	from	application	300:310	arg1	treatment					356:364	treatment	356:364	treatment	356:364	Increasing evidence is supporting the potential application of natural polysaccharides in prevention or treatment of PD.
32933702	2	53	from	application	300:310	arg1	prevention					342:351	prevention	342:351	prevention	342:351	Increasing evidence is supporting the potential application of natural polysaccharides in prevention or treatment of PD.
32933702	4	54	theme	nuclear	650:656	arg1	NMR					678:680	NMR	678:680	NMR	678:680	Structural features of LFP-1 were analyzed with molecular weight (MW) distribution, monosaccharide composition, methylation and nuclear magnetic resonance (NMR) spectra.
32933702	4	54	theme	nuclear	650:656	arg1	resonance					667:675	nuclear magnetic resonance	650:675	nuclear magnetic resonance (NMR) spectra	650:689	Structural features of LFP-1 were analyzed with molecular weight (MW) distribution, monosaccharide composition, methylation and nuclear magnetic resonance (NMR) spectra.
32933702	5	55	theme	Da	775:776	arg1	MW					758:759	an average MW	747:759	an average MW of 1.78 × 104 Da	747:776	LFP-1 was a complicated structured polysaccharide with an average MW of 1.78 × 104 Da and composed of highly branched arabinogalactans, homogalacturonan and rhamnogalacturonan moieties.
32933702	2	56	theme	Increasing	252:261	arg1	evidence					263:270	Increasing evidence	252:270	Increasing evidence	252:270	Increasing evidence is supporting the potential application of natural polysaccharides in prevention or treatment of PD.
32933702	3	57	theme	acidic	391:396	arg1	LFP-1					419:423	an acidic heteropolysaccharide LFP-1	388:423	an acidic heteropolysaccharide LFP-1	388:423	In this study, an acidic heteropolysaccharide LFP-1 was isolated from Lycii fructus, and purified by ion-exchange and gel filtration chromatography.
33255007	8	0	theme	tensile	1394:1400	arg1	strength					1402:1409	the tensile strength	1390:1409	the tensile strength	1390:1409	Growing glycerol ratio not only enhances the elongation at break and diffusion rate, but it also changes the tensile strength and the remaining weight.
33255007	7	1	theme	physical	1183:1190	arg1	cross-linking					1192:1204	adequate physical cross-linking	1174:1204	adequate physical cross-linking with γ-PGA	1174:1215	Our results indicate that adequate physical cross-linking with γ-PGA improves the original mechanical properties and delays degradation.
33255007	8	2	from	break	1344:1348	arg1	elongation					1330:1339	the elongation	1326:1339	the elongation at break and diffusion rate	1326:1367	Growing glycerol ratio not only enhances the elongation at break and diffusion rate, but it also changes the tensile strength and the remaining weight.
33255007	2	3	theme	calcium-form	355:366	arg1	γ-PGA					390:394	γ-PGA	390:394	γ-PGA	390:394	In the present study, a functional hydrogel-based barrier membrane is fabricated using calcium-form poly-γ-glutamic acid (γ-PGA) and glycerol blending into gellan gum.
33255007	2	3	theme	calcium-form	355:366	arg1	acid					384:387	calcium-form poly-γ-glutamic acid	355:387	calcium-form poly-γ-glutamic acid (γ-PGA)	355:395	In the present study, a functional hydrogel-based barrier membrane is fabricated using calcium-form poly-γ-glutamic acid (γ-PGA) and glycerol blending into gellan gum.
33255007	11	4	theme	osteoblastic	1866:1877	arg1	responses					1879:1887	positive osteoblastic responses	1857:1887	positive osteoblastic responses	1857:1887	In general, these tunable materials with biocompatibility, biodegradability, and positive osteoblastic responses were poised to be possible candidates for bone defect repair.
33255007	0	5	theme	bone	71:74	arg1	regeneration					76:87	guided bone regeneration	64:87	guided bone regeneration	64:87	A novel γ-PGA composite gellan membrane containing glycerol for guided bone regeneration.
33255007	6	6	dep	biocompatibility	1029:1044	arg1	The					1025:1027	The	1025:1027	The	1025:1027	The biocompatibility, proliferation, and osteoblastic response of membranes were examined by osteoblast-like (MG63) cells.
33255007	10	7	theme	osteoblastic	1753:1764	arg1	response					1766:1773	osteoblastic response	1753:1773	osteoblastic response	1753:1773	However, worth noting is the glycerol-modified membrane cannot bear a close resemblance with the non-glycerol group in the high level of osteoblastic response.
33255007	7	8	with	cross-linking	1192:1204	arg1	γ-PGA					1211:1215	γ-PGA	1211:1215	γ-PGA	1211:1215	Our results indicate that adequate physical cross-linking with γ-PGA improves the original mechanical properties and delays degradation.
33255007	9	9	theme	alkaline	1569:1576	arg1	ALP					1591:1593	ALP	1591:1593	ALP	1591:1593	In vitro biocompatibility tests, an adequate ratio of γ-PGA modification significantly enhances the proliferation, the secretion of alkaline phosphatase (ALP) and mineralization.
33255007	9	9	theme	alkaline	1569:1576	arg1	phosphatase					1578:1588	alkaline phosphatase	1569:1588	alkaline phosphatase (ALP)	1569:1594	In vitro biocompatibility tests, an adequate ratio of γ-PGA modification significantly enhances the proliferation, the secretion of alkaline phosphatase (ALP) and mineralization.
33255007	11	10	theme	positive	1857:1864	arg1	responses					1879:1887	positive osteoblastic responses	1857:1887	positive osteoblastic responses	1857:1887	In general, these tunable materials with biocompatibility, biodegradability, and positive osteoblastic responses were poised to be possible candidates for bone defect repair.
33255007	6	11	theme	membranes	1091:1099	arg1	response					1079:1086	osteoblastic response	1066:1086	osteoblastic response	1066:1086	The biocompatibility, proliferation, and osteoblastic response of membranes were examined by osteoblast-like (MG63) cells.
33255007	6	11	theme	membranes	1091:1099	arg1	proliferation					1047:1059	proliferation	1047:1059	proliferation	1047:1059	The biocompatibility, proliferation, and osteoblastic response of membranes were examined by osteoblast-like (MG63) cells.
33255007	6	11	theme	membranes	1091:1099	arg1	biocompatibility					1029:1044	biocompatibility	1029:1044	biocompatibility	1029:1044	The biocompatibility, proliferation, and osteoblastic response of membranes were examined by osteoblast-like (MG63) cells.
33255007	5	12	theme	swelling	944:951	arg1	behavior					953:960	swelling behavior	944:960	swelling behavior	944:960	Our composite gellan gum-based membranes were tested including to their morphology, mechanical properties, swelling behavior, protein adsorption, drug diffusion, and lysozyme degradation.
33255007	5	13	theme	lysozyme	1003:1010	arg1	degradation					1012:1022	lysozyme degradation	1003:1022	lysozyme degradation	1003:1022	Our composite gellan gum-based membranes were tested including to their morphology, mechanical properties, swelling behavior, protein adsorption, drug diffusion, and lysozyme degradation.
33255007	9	14	theme	γ-PGA	1491:1495	arg1	modification					1497:1508	γ-PGA modification	1491:1508	γ-PGA modification	1491:1508	In vitro biocompatibility tests, an adequate ratio of γ-PGA modification significantly enhances the proliferation, the secretion of alkaline phosphatase (ALP) and mineralization.
33255007	10	15	theme	glycerol-modified	1645:1661	arg1	worth					1625:1629	worth	1625:1629	worth noting	1625:1636	However, worth noting is the glycerol-modified membrane cannot bear a close resemblance with the non-glycerol group in the high level of osteoblastic response.
33255007	10	15	theme	glycerol-modified	1645:1661	arg1	membrane					1663:1670	the glycerol-modified membrane	1641:1670	the glycerol-modified membrane cannot bear a close resemblance with the non-glycerol group in the high level of osteoblastic response	1641:1773	However, worth noting is the glycerol-modified membrane cannot bear a close resemblance with the non-glycerol group in the high level of osteoblastic response.
33255007	7	16	theme	adequate	1174:1181	arg1	cross-linking					1192:1204	adequate physical cross-linking	1174:1204	adequate physical cross-linking with γ-PGA	1174:1215	Our results indicate that adequate physical cross-linking with γ-PGA improves the original mechanical properties and delays degradation.
33255007	2	17	theme	present	275:281	arg1	study					283:287	the present study	271:287	the present study	271:287	In the present study, a functional hydrogel-based barrier membrane is fabricated using calcium-form poly-γ-glutamic acid (γ-PGA) and glycerol blending into gellan gum.
33255007	5	18	theme	drug	983:986	arg1	diffusion					988:996	drug diffusion	983:996	drug diffusion	983:996	Our composite gellan gum-based membranes were tested including to their morphology, mechanical properties, swelling behavior, protein adsorption, drug diffusion, and lysozyme degradation.
33255007	10	19	theme	close	1686:1690	arg1	resemblance					1692:1702	a close resemblance	1684:1702	a close resemblance with the non-glycerol group	1684:1730	However, worth noting is the glycerol-modified membrane cannot bear a close resemblance with the non-glycerol group in the high level of osteoblastic response.
33255007	4	20	theme	excellent	748:756	arg1	characteristics					758:772	excellent characteristics	748:772	excellent characteristics	748:772	Gellan gum-based membranes with uniformly distributed calcium aggregates are not only successfully manufactured but also providing excellent characteristics for protein adsorption, bioactivity, and bone cell maturation.
33255007	7	21	theme	mechanical	1239:1248	arg1	properties					1250:1259	the original mechanical properties	1226:1259	the original mechanical properties	1226:1259	Our results indicate that adequate physical cross-linking with γ-PGA improves the original mechanical properties and delays degradation.
33255007	5	22	theme	gellan	851:856	arg1	membranes					868:876	Our composite gellan gum-based membranes	837:876	Our composite gellan gum-based membranes	837:876	Our composite gellan gum-based membranes were tested including to their morphology, mechanical properties, swelling behavior, protein adsorption, drug diffusion, and lysozyme degradation.
33255007	4	23	theme	cell	820:823	arg1	maturation					825:834	bone cell maturation	815:834	bone cell maturation	815:834	Gellan gum-based membranes with uniformly distributed calcium aggregates are not only successfully manufactured but also providing excellent characteristics for protein adsorption, bioactivity, and bone cell maturation.
33255007	6	24	theme	MG63	1135:1138	arg1	cells					1141:1145	osteoblast-like (MG63) cells	1118:1145	osteoblast-like (MG63) cells	1118:1145	The biocompatibility, proliferation, and osteoblastic response of membranes were examined by osteoblast-like (MG63) cells.
33255007	1	25	theme	delivery	160:167	arg1	vehicle					169:175	a delivery vehicle	158:175	a delivery vehicle for transporting drugs and osteoinductive factors	158:225	An ideal barrier membrane design should incorporate the function of a delivery vehicle for transporting drugs and osteoinductive factors to where the body is under inflammation.
33255007	9	26	theme	modification	1497:1508	arg1	ratio					1482:1486	an adequate ratio	1470:1486	an adequate ratio of γ-PGA modification	1470:1508	In vitro biocompatibility tests, an adequate ratio of γ-PGA modification significantly enhances the proliferation, the secretion of alkaline phosphatase (ALP) and mineralization.
33255007	4	27	theme	bone	815:818	arg1	maturation					825:834	bone cell maturation	815:834	bone cell maturation	815:834	Gellan gum-based membranes with uniformly distributed calcium aggregates are not only successfully manufactured but also providing excellent characteristics for protein adsorption, bioactivity, and bone cell maturation.
33255007	11	28	with	materials	1802:1810	arg1	biodegradability					1835:1850	biodegradability	1835:1850	biodegradability	1835:1850	In general, these tunable materials with biocompatibility, biodegradability, and positive osteoblastic responses were poised to be possible candidates for bone defect repair.
33255007	11	28	with	materials	1802:1810	arg1	biocompatibility					1817:1832	biocompatibility	1817:1832	biocompatibility	1817:1832	In general, these tunable materials with biocompatibility, biodegradability, and positive osteoblastic responses were poised to be possible candidates for bone defect repair.
33255007	11	28	with	materials	1802:1810	arg1	responses					1879:1887	positive osteoblastic responses	1857:1887	positive osteoblastic responses	1857:1887	In general, these tunable materials with biocompatibility, biodegradability, and positive osteoblastic responses were poised to be possible candidates for bone defect repair.
33255007	1	29	theme	vehicle	169:175	arg1	function					146:153	the function	142:153	the function of a delivery vehicle for transporting drugs and osteoinductive factors to where the body is under inflammation	142:265	An ideal barrier membrane design should incorporate the function of a delivery vehicle for transporting drugs and osteoinductive factors to where the body is under inflammation.
33255007	0	30	theme	novel	2:6	arg1	membrane					31:38	A novel γ-PGA composite gellan membrane	0:38	A novel γ-PGA composite gellan membrane	0:38	A novel γ-PGA composite gellan membrane containing glycerol for guided bone regeneration.
33255007	2	31	theme	hydrogel-based	303:316	arg1	barrier					318:324	a functional hydrogel-based barrier	290:324	a functional hydrogel-based barrier membrane	290:333	In the present study, a functional hydrogel-based barrier membrane is fabricated using calcium-form poly-γ-glutamic acid (γ-PGA) and glycerol blending into gellan gum.
33255007	5	32	theme	mechanical	921:930	arg1	properties					932:941	mechanical properties	921:941	mechanical properties	921:941	Our composite gellan gum-based membranes were tested including to their morphology, mechanical properties, swelling behavior, protein adsorption, drug diffusion, and lysozyme degradation.
33255007	5	33	theme	gum-based	858:866	arg1	membranes					868:876	Our composite gellan gum-based membranes	837:876	Our composite gellan gum-based membranes	837:876	Our composite gellan gum-based membranes were tested including to their morphology, mechanical properties, swelling behavior, protein adsorption, drug diffusion, and lysozyme degradation.
33255007	11	34	theme	tunable	1794:1800	arg1	materials					1802:1810	these tunable materials	1788:1810	these tunable materials with biocompatibility, biodegradability, and positive osteoblastic responses	1788:1887	In general, these tunable materials with biocompatibility, biodegradability, and positive osteoblastic responses were poised to be possible candidates for bone defect repair.
33255007	2	35	theme	functional	292:301	arg1	barrier					318:324	a functional hydrogel-based barrier	290:324	a functional hydrogel-based barrier membrane	290:333	In the present study, a functional hydrogel-based barrier membrane is fabricated using calcium-form poly-γ-glutamic acid (γ-PGA) and glycerol blending into gellan gum.
33255007	1	36	theme	transporting	181:192	arg1	drugs					194:198	transporting drugs	181:198	transporting drugs	181:198	An ideal barrier membrane design should incorporate the function of a delivery vehicle for transporting drugs and osteoinductive factors to where the body is under inflammation.
33255007	0	37	theme	composite	14:22	arg1	membrane					31:38	A novel γ-PGA composite gellan membrane	0:38	A novel γ-PGA composite gellan membrane	0:38	A novel γ-PGA composite gellan membrane containing glycerol for guided bone regeneration.
33255007	4	38	theme	protein	778:784	arg1	adsorption					786:795	protein adsorption	778:795	protein adsorption	778:795	Gellan gum-based membranes with uniformly distributed calcium aggregates are not only successfully manufactured but also providing excellent characteristics for protein adsorption, bioactivity, and bone cell maturation.
33255007	10	39	theme	response	1766:1773	arg1	level					1744:1748	the high level	1735:1748	the high level of osteoblastic response	1735:1773	However, worth noting is the glycerol-modified membrane cannot bear a close resemblance with the non-glycerol group in the high level of osteoblastic response.
33255007	8	40	theme	Growing	1285:1291	arg1	ratio					1302:1306	Growing glycerol ratio	1285:1306	Growing glycerol ratio	1285:1306	Growing glycerol ratio not only enhances the elongation at break and diffusion rate, but it also changes the tensile strength and the remaining weight.
33255007	1	41	theme	ideal	93:97	arg1	design					116:121	An ideal barrier membrane design	90:121	An ideal barrier membrane design	90:121	An ideal barrier membrane design should incorporate the function of a delivery vehicle for transporting drugs and osteoinductive factors to where the body is under inflammation.
33255007	4	42	with	membranes	634:642	arg1	aggregates					679:688	uniformly distributed calcium aggregates	649:688	uniformly distributed calcium aggregates	649:688	Gellan gum-based membranes with uniformly distributed calcium aggregates are not only successfully manufactured but also providing excellent characteristics for protein adsorption, bioactivity, and bone cell maturation.
33255007	2	43	theme	gellan	424:429	arg1	gum					431:433	gellan gum	424:433	gellan gum	424:433	In the present study, a functional hydrogel-based barrier membrane is fabricated using calcium-form poly-γ-glutamic acid (γ-PGA) and glycerol blending into gellan gum.
33255007	0	44	theme	γ-PGA	8:12	arg1	membrane					31:38	A novel γ-PGA composite gellan membrane	0:38	A novel γ-PGA composite gellan membrane	0:38	A novel γ-PGA composite gellan membrane containing glycerol for guided bone regeneration.
33255007	1	45	theme	barrier	99:105	arg1	design					116:121	An ideal barrier membrane design	90:121	An ideal barrier membrane design	90:121	An ideal barrier membrane design should incorporate the function of a delivery vehicle for transporting drugs and osteoinductive factors to where the body is under inflammation.
33255007	3	46	theme	poly-γ-glutamic	474:488	arg1	γ-PGA					496:500	γ-PGA	496:500	γ-PGA	496:500	The concentration of the calcium-form poly-γ-glutamic acid (γ-PGA) and the glycerol ratio are studied for improving practicability in easy-handling and expanding the coverage area.
33255007	3	46	theme	poly-γ-glutamic	474:488	arg1	acid					490:493	the calcium-form poly-γ-glutamic acid	457:493	the calcium-form poly-γ-glutamic acid (γ-PGA)	457:501	The concentration of the calcium-form poly-γ-glutamic acid (γ-PGA) and the glycerol ratio are studied for improving practicability in easy-handling and expanding the coverage area.
33255007	10	47	theme	non-glycerol	1713:1724	arg1	group					1726:1730	the non-glycerol group	1709:1730	the non-glycerol group	1709:1730	However, worth noting is the glycerol-modified membrane cannot bear a close resemblance with the non-glycerol group in the high level of osteoblastic response.
33255007	8	48	dep	ratio	1302:1306	arg1	only					1312:1315	only	1312:1315	only	1312:1315	Growing glycerol ratio not only enhances the elongation at break and diffusion rate, but it also changes the tensile strength and the remaining weight.
33255007	4	49	theme	calcium	671:677	arg1	aggregates					679:688	uniformly distributed calcium aggregates	649:688	uniformly distributed calcium aggregates	649:688	Gellan gum-based membranes with uniformly distributed calcium aggregates are not only successfully manufactured but also providing excellent characteristics for protein adsorption, bioactivity, and bone cell maturation.
33255007	3	50	theme	coverage	602:609	arg1	area					611:614	the coverage area	598:614	the coverage area	598:614	The concentration of the calcium-form poly-γ-glutamic acid (γ-PGA) and the glycerol ratio are studied for improving practicability in easy-handling and expanding the coverage area.
33255007	1	51	theme	osteoinductive	204:217	arg1	factors					219:225	osteoinductive factors	204:225	osteoinductive factors	204:225	An ideal barrier membrane design should incorporate the function of a delivery vehicle for transporting drugs and osteoinductive factors to where the body is under inflammation.
33255007	0	52	theme	gellan	24:29	arg1	membrane					31:38	A novel γ-PGA composite gellan membrane	0:38	A novel γ-PGA composite gellan membrane	0:38	A novel γ-PGA composite gellan membrane containing glycerol for guided bone regeneration.
33255007	8	53	theme	glycerol	1293:1300	arg1	ratio					1302:1306	Growing glycerol ratio	1285:1306	Growing glycerol ratio	1285:1306	Growing glycerol ratio not only enhances the elongation at break and diffusion rate, but it also changes the tensile strength and the remaining weight.
33255007	7	54	theme	original	1230:1237	arg1	properties					1250:1259	the original mechanical properties	1226:1259	the original mechanical properties	1226:1259	Our results indicate that adequate physical cross-linking with γ-PGA improves the original mechanical properties and delays degradation.
33255007	4	55	theme	distributed	659:669	arg1	aggregates					679:688	uniformly distributed calcium aggregates	649:688	uniformly distributed calcium aggregates	649:688	Gellan gum-based membranes with uniformly distributed calcium aggregates are not only successfully manufactured but also providing excellent characteristics for protein adsorption, bioactivity, and bone cell maturation.
33255007	11	56	theme	bone	1931:1934	arg1	repair					1943:1948	bone defect repair	1931:1948	bone defect repair	1931:1948	In general, these tunable materials with biocompatibility, biodegradability, and positive osteoblastic responses were poised to be possible candidates for bone defect repair.
33255007	1	57	theme	membrane	107:114	arg1	design					116:121	An ideal barrier membrane design	90:121	An ideal barrier membrane design	90:121	An ideal barrier membrane design should incorporate the function of a delivery vehicle for transporting drugs and osteoinductive factors to where the body is under inflammation.
33255007	5	58	theme	composite	841:849	arg1	membranes					868:876	Our composite gellan gum-based membranes	837:876	Our composite gellan gum-based membranes	837:876	Our composite gellan gum-based membranes were tested including to their morphology, mechanical properties, swelling behavior, protein adsorption, drug diffusion, and lysozyme degradation.
33255007	9	59	theme	vitro	1440:1444	arg1	tests					1463:1467	vitro biocompatibility tests	1440:1467	vitro biocompatibility tests	1440:1467	In vitro biocompatibility tests, an adequate ratio of γ-PGA modification significantly enhances the proliferation, the secretion of alkaline phosphatase (ALP) and mineralization.
33255007	9	60	theme	phosphatase	1578:1588	arg1	secretion					1556:1564	the secretion	1552:1564	the secretion of alkaline phosphatase (ALP)	1552:1594	In vitro biocompatibility tests, an adequate ratio of γ-PGA modification significantly enhances the proliferation, the secretion of alkaline phosphatase (ALP) and mineralization.
33255007	9	60	theme	phosphatase	1578:1588	arg1	proliferation					1537:1549	the proliferation	1533:1549	the proliferation	1533:1549	In vitro biocompatibility tests, an adequate ratio of γ-PGA modification significantly enhances the proliferation, the secretion of alkaline phosphatase (ALP) and mineralization.
33255007	9	60	theme	phosphatase	1578:1588	arg1	mineralization					1600:1613	mineralization	1600:1613	mineralization	1600:1613	In vitro biocompatibility tests, an adequate ratio of γ-PGA modification significantly enhances the proliferation, the secretion of alkaline phosphatase (ALP) and mineralization.
33255007	3	61	from	practicability	552:565	arg1	easy-handling					570:582	easy-handling	570:582	easy-handling	570:582	The concentration of the calcium-form poly-γ-glutamic acid (γ-PGA) and the glycerol ratio are studied for improving practicability in easy-handling and expanding the coverage area.
33255007	9	62	theme	biocompatibility	1446:1461	arg1	tests					1463:1467	vitro biocompatibility tests	1440:1467	vitro biocompatibility tests	1440:1467	In vitro biocompatibility tests, an adequate ratio of γ-PGA modification significantly enhances the proliferation, the secretion of alkaline phosphatase (ALP) and mineralization.
33255007	10	63	with	resemblance	1692:1702	arg1	group					1726:1730	the non-glycerol group	1709:1730	the non-glycerol group	1709:1730	However, worth noting is the glycerol-modified membrane cannot bear a close resemblance with the non-glycerol group in the high level of osteoblastic response.
33255007	10	64	contain	bear	1679:1682	arg1	cannot					1672:1677	cannot	1672:1677	cannot	1672:1677	However, worth noting is the glycerol-modified membrane cannot bear a close resemblance with the non-glycerol group in the high level of osteoblastic response.
33255007	10	64	contain	bear	1679:1682	arg3	level					1744:1748	the high level	1735:1748	the high level of osteoblastic response	1735:1773	However, worth noting is the glycerol-modified membrane cannot bear a close resemblance with the non-glycerol group in the high level of osteoblastic response.
33255007	10	64	contain	bear	1679:1682	arg2	resemblance					1692:1702	a close resemblance	1684:1702	a close resemblance with the non-glycerol group	1684:1730	However, worth noting is the glycerol-modified membrane cannot bear a close resemblance with the non-glycerol group in the high level of osteoblastic response.
33255007	3	65	theme	acid	490:493	arg1	concentration					440:452	The concentration	436:452	The concentration of the calcium-form poly-γ-glutamic acid (γ-PGA)	436:501	The concentration of the calcium-form poly-γ-glutamic acid (γ-PGA) and the glycerol ratio are studied for improving practicability in easy-handling and expanding the coverage area.
33255007	3	65	theme	acid	490:493	arg1	ratio					520:524	the glycerol ratio	507:524	the glycerol ratio	507:524	The concentration of the calcium-form poly-γ-glutamic acid (γ-PGA) and the glycerol ratio are studied for improving practicability in easy-handling and expanding the coverage area.
33255007	4	66	theme	Gellan	617:622	arg1	membranes					634:642	Gellan gum-based membranes	617:642	Gellan gum-based membranes with uniformly distributed calcium aggregates	617:688	Gellan gum-based membranes with uniformly distributed calcium aggregates are not only successfully manufactured but also providing excellent characteristics for protein adsorption, bioactivity, and bone cell maturation.
33255007	8	67	theme	remaining	1419:1427	arg1	weight					1429:1434	the remaining weight	1415:1434	the remaining weight	1415:1434	Growing glycerol ratio not only enhances the elongation at break and diffusion rate, but it also changes the tensile strength and the remaining weight.
33255007	5	68	theme	protein	963:969	arg1	adsorption					971:980	protein adsorption	963:980	protein adsorption	963:980	Our composite gellan gum-based membranes were tested including to their morphology, mechanical properties, swelling behavior, protein adsorption, drug diffusion, and lysozyme degradation.
33255007	6	69	theme	osteoblast-like	1118:1132	arg1	cells					1141:1145	osteoblast-like (MG63) cells	1118:1145	osteoblast-like (MG63) cells	1118:1145	The biocompatibility, proliferation, and osteoblastic response of membranes were examined by osteoblast-like (MG63) cells.
33255007	8	70	from	rate	1364:1367	arg1	elongation					1330:1339	the elongation	1326:1339	the elongation at break and diffusion rate	1326:1367	Growing glycerol ratio not only enhances the elongation at break and diffusion rate, but it also changes the tensile strength and the remaining weight.
33255007	2	71	theme	barrier	318:324	arg1	membrane					326:333	a functional hydrogel-based barrier membrane	290:333	a functional hydrogel-based barrier membrane	290:333	In the present study, a functional hydrogel-based barrier membrane is fabricated using calcium-form poly-γ-glutamic acid (γ-PGA) and glycerol blending into gellan gum.
33255007	11	72	theme	possible	1907:1914	arg1	candidates					1916:1925	possible candidates	1907:1925	possible candidates for bone defect repair	1907:1948	In general, these tunable materials with biocompatibility, biodegradability, and positive osteoblastic responses were poised to be possible candidates for bone defect repair.
33255007	11	73	theme	defect	1936:1941	arg1	repair					1943:1948	bone defect repair	1931:1948	bone defect repair	1931:1948	In general, these tunable materials with biocompatibility, biodegradability, and positive osteoblastic responses were poised to be possible candidates for bone defect repair.
33255007	0	74	theme	guided	64:69	arg1	regeneration					76:87	guided bone regeneration	64:87	guided bone regeneration	64:87	A novel γ-PGA composite gellan membrane containing glycerol for guided bone regeneration.
33255007	8	75	theme	diffusion	1354:1362	arg1	rate					1364:1367	diffusion rate	1354:1367	diffusion rate	1354:1367	Growing glycerol ratio not only enhances the elongation at break and diffusion rate, but it also changes the tensile strength and the remaining weight.
33255007	3	76	theme	calcium-form	461:472	arg1	γ-PGA					496:500	γ-PGA	496:500	γ-PGA	496:500	The concentration of the calcium-form poly-γ-glutamic acid (γ-PGA) and the glycerol ratio are studied for improving practicability in easy-handling and expanding the coverage area.
33255007	3	76	theme	calcium-form	461:472	arg1	acid					490:493	the calcium-form poly-γ-glutamic acid	457:493	the calcium-form poly-γ-glutamic acid (γ-PGA)	457:501	The concentration of the calcium-form poly-γ-glutamic acid (γ-PGA) and the glycerol ratio are studied for improving practicability in easy-handling and expanding the coverage area.
33255007	3	77	theme	glycerol	511:518	arg1	ratio					520:524	the glycerol ratio	507:524	the glycerol ratio	507:524	The concentration of the calcium-form poly-γ-glutamic acid (γ-PGA) and the glycerol ratio are studied for improving practicability in easy-handling and expanding the coverage area.
33255007	10	78	theme	high	1739:1742	arg1	level					1744:1748	the high level	1735:1748	the high level of osteoblastic response	1735:1773	However, worth noting is the glycerol-modified membrane cannot bear a close resemblance with the non-glycerol group in the high level of osteoblastic response.
33255007	6	79	theme	osteoblastic	1066:1077	arg1	response					1079:1086	osteoblastic response	1066:1086	osteoblastic response	1066:1086	The biocompatibility, proliferation, and osteoblastic response of membranes were examined by osteoblast-like (MG63) cells.
33255007	2	80	theme	poly-γ-glutamic	368:382	arg1	γ-PGA					390:394	γ-PGA	390:394	γ-PGA	390:394	In the present study, a functional hydrogel-based barrier membrane is fabricated using calcium-form poly-γ-glutamic acid (γ-PGA) and glycerol blending into gellan gum.
33255007	2	80	theme	poly-γ-glutamic	368:382	arg1	acid					384:387	calcium-form poly-γ-glutamic acid	355:387	calcium-form poly-γ-glutamic acid (γ-PGA)	355:395	In the present study, a functional hydrogel-based barrier membrane is fabricated using calcium-form poly-γ-glutamic acid (γ-PGA) and glycerol blending into gellan gum.
33255007	9	81	theme	adequate	1473:1480	arg1	ratio					1482:1486	an adequate ratio	1470:1486	an adequate ratio of γ-PGA modification	1470:1508	In vitro biocompatibility tests, an adequate ratio of γ-PGA modification significantly enhances the proliferation, the secretion of alkaline phosphatase (ALP) and mineralization.
33255007	4	82	theme	gum-based	624:632	arg1	membranes					634:642	Gellan gum-based membranes	617:642	Gellan gum-based membranes with uniformly distributed calcium aggregates	617:688	Gellan gum-based membranes with uniformly distributed calcium aggregates are not only successfully manufactured but also providing excellent characteristics for protein adsorption, bioactivity, and bone cell maturation.
33340634	0	0	theme	curcumin	91:98	arg1	encapsulation					100:112	curcumin encapsulation	91:112	curcumin encapsulation	91:112	Development and characterization of soybean protein isolate and fucoidan nanoparticles for curcumin encapsulation.
33340634	7	1	theme	simple	1222:1227	arg1	method					1229:1234	a simple method	1220:1234	a simple method	1220:1234	Thus, we provide a simple method for Cur delivery, which can also be potentially used for delivering lipid-soluble active ingredients.
33340634	4	2	theme	infrared	733:740	arg1	spectroscopy					742:753	Fourier-transform infrared spectroscopy	715:753	Fourier-transform infrared spectroscopy	715:753	The structure of the Spi-Fuc nanoparticles was studied via Fourier-transform infrared spectroscopy, transmission electron microscopy, and scanning electron microscopy.
33340634	6	3	theme	spherical	1035:1043	arg1	structure					1057:1065	a spherical, core-shell structure	1033:1065	a spherical, core-shell structure	1033:1065	Our results showed that the Spi-Fuc nanoparticles (size, approximately 236.56 nm) had a spherical, core-shell structure and that they could effectively load Cur with an embedding efficiency of >95%; moreover, the system had long-term dispersion stability.
33340634	6	4	dep	spherical	1035:1043	arg1	core-shell					1046:1055	core-shell	1046:1055	core-shell	1046:1055	Our results showed that the Spi-Fuc nanoparticles (size, approximately 236.56 nm) had a spherical, core-shell structure and that they could effectively load Cur with an embedding efficiency of >95%; moreover, the system had long-term dispersion stability.
33340634	3	5	theme	polymer	511:517	arg1	fucoidan					481:488	fucoidan	481:488	fucoidan (Fuc)	481:494	Soybean protein isolate (Spi) and fucoidan (Fuc) were used as a polymer matrix, and core-shell nanoparticles were prepared to encapsulate Cur via electrostatic interaction under acidic and neutral conditions.
33340634	3	5	theme	polymer	511:517	arg1	isolate					463:469	Soybean protein isolate	447:469	Soybean protein isolate (Spi)	447:475	Soybean protein isolate (Spi) and fucoidan (Fuc) were used as a polymer matrix, and core-shell nanoparticles were prepared to encapsulate Cur via electrostatic interaction under acidic and neutral conditions.
33340634	3	5	theme	polymer	511:517	arg1	matrix					519:524	a polymer matrix	509:524	a polymer matrix	509:524	Soybean protein isolate (Spi) and fucoidan (Fuc) were used as a polymer matrix, and core-shell nanoparticles were prepared to encapsulate Cur via electrostatic interaction under acidic and neutral conditions.
33340634	7	6	used	used	1284:1287	arg2	method					1229:1234	a simple method	1220:1234	a simple method	1220:1234	Thus, we provide a simple method for Cur delivery, which can also be potentially used for delivering lipid-soluble active ingredients.
33340634	4	7	theme	Spi-Fuc	677:683	arg1	nanoparticles					685:697	the Spi-Fuc nanoparticles	673:697	the Spi-Fuc nanoparticles	673:697	The structure of the Spi-Fuc nanoparticles was studied via Fourier-transform infrared spectroscopy, transmission electron microscopy, and scanning electron microscopy.
33340634	1	8	theme	low	263:265	arg1	solubility					267:276	its low solubility	259:276	its low solubility	259:276	Curcumin (Cur) is a natural polyphenol with beneficial biological and pharmacological activities; however, it has limited applications owing to its low solubility and light sensitivity.
33340634	1	9	with	polyphenol	143:152	arg1	activities					201:210	beneficial biological and pharmacological activities	159:210	beneficial biological and pharmacological activities	159:210	Curcumin (Cur) is a natural polyphenol with beneficial biological and pharmacological activities; however, it has limited applications owing to its low solubility and light sensitivity.
33340634	4	10	theme	electron	803:810	arg1	microscopy					812:821	scanning electron microscopy	794:821	scanning electron microscopy	794:821	The structure of the Spi-Fuc nanoparticles was studied via Fourier-transform infrared spectroscopy, transmission electron microscopy, and scanning electron microscopy.
33340634	3	11	theme	electrostatic	593:605	arg1	interaction					607:617	electrostatic interaction	593:617	electrostatic interaction	593:617	Soybean protein isolate (Spi) and fucoidan (Fuc) were used as a polymer matrix, and core-shell nanoparticles were prepared to encapsulate Cur via electrostatic interaction under acidic and neutral conditions.
33340634	5	12	theme	drug	905:908	arg1	rate					918:921	drug loading rate	905:921	drug loading rate	905:921	Concurrently, we evaluated the efficacy of the nanoparticles based on stability, drug loading rate, and simulated release.
33340634	4	13	theme	Fourier-transform	715:731	arg1	spectroscopy					742:753	Fourier-transform infrared spectroscopy	715:753	Fourier-transform infrared spectroscopy	715:753	The structure of the Spi-Fuc nanoparticles was studied via Fourier-transform infrared spectroscopy, transmission electron microscopy, and scanning electron microscopy.
33340634	1	14	theme	biological	170:179	arg1	activities					201:210	beneficial biological and pharmacological activities	159:210	beneficial biological and pharmacological activities	159:210	Curcumin (Cur) is a natural polyphenol with beneficial biological and pharmacological activities; however, it has limited applications owing to its low solubility and light sensitivity.
33340634	3	15	theme	Soybean	447:453	arg1	Spi					472:474	Spi	472:474	Spi	472:474	Soybean protein isolate (Spi) and fucoidan (Fuc) were used as a polymer matrix, and core-shell nanoparticles were prepared to encapsulate Cur via electrostatic interaction under acidic and neutral conditions.
33340634	3	15	theme	Soybean	447:453	arg1	fucoidan					481:488	fucoidan	481:488	fucoidan (Fuc)	481:494	Soybean protein isolate (Spi) and fucoidan (Fuc) were used as a polymer matrix, and core-shell nanoparticles were prepared to encapsulate Cur via electrostatic interaction under acidic and neutral conditions.
33340634	3	15	theme	Soybean	447:453	arg1	isolate					463:469	Soybean protein isolate	447:469	Soybean protein isolate (Spi)	447:475	Soybean protein isolate (Spi) and fucoidan (Fuc) were used as a polymer matrix, and core-shell nanoparticles were prepared to encapsulate Cur via electrostatic interaction under acidic and neutral conditions.
33340634	3	15	theme	Soybean	447:453	arg1	matrix					519:524	a polymer matrix	509:524	a polymer matrix	509:524	Soybean protein isolate (Spi) and fucoidan (Fuc) were used as a polymer matrix, and core-shell nanoparticles were prepared to encapsulate Cur via electrostatic interaction under acidic and neutral conditions.
33340634	3	16	theme	core-shell	531:540	arg1	nanoparticles					542:554	core-shell nanoparticles	531:554	core-shell nanoparticles	531:554	Soybean protein isolate (Spi) and fucoidan (Fuc) were used as a polymer matrix, and core-shell nanoparticles were prepared to encapsulate Cur via electrostatic interaction under acidic and neutral conditions.
33340634	6	17	theme	embedding	1116:1124	arg1	efficiency					1126:1135	an embedding efficiency	1113:1135	an embedding efficiency of >95%	1113:1143	Our results showed that the Spi-Fuc nanoparticles (size, approximately 236.56 nm) had a spherical, core-shell structure and that they could effectively load Cur with an embedding efficiency of >95%; moreover, the system had long-term dispersion stability.
33340634	5	18	theme	loading	910:916	arg1	rate					918:921	drug loading rate	905:921	drug loading rate	905:921	Concurrently, we evaluated the efficacy of the nanoparticles based on stability, drug loading rate, and simulated release.
33340634	6	19	theme	long-term	1171:1179	arg1	stability					1192:1200	long-term dispersion stability	1171:1200	long-term dispersion stability	1171:1200	Our results showed that the Spi-Fuc nanoparticles (size, approximately 236.56 nm) had a spherical, core-shell structure and that they could effectively load Cur with an embedding efficiency of >95%; moreover, the system had long-term dispersion stability.
33340634	1	20	theme	pharmacological	185:199	arg1	activities					201:210	beneficial biological and pharmacological activities	159:210	beneficial biological and pharmacological activities	159:210	Curcumin (Cur) is a natural polyphenol with beneficial biological and pharmacological activities; however, it has limited applications owing to its low solubility and light sensitivity.
33340634	2	21	from	stability	396:404	arg1	solutions					436:444	aqueous solutions	428:444	aqueous solutions	428:444	The protein-polysaccharide complex can effectively embed lipid-soluble drugs to increase their stability and dispensability in aqueous solutions.
33340634	6	22	contain	had	1029:1031	arg1	nanoparticles					983:995	the Spi-Fuc nanoparticles	971:995	the Spi-Fuc nanoparticles (size, approximately 236.56 nm)	971:1027	Our results showed that the Spi-Fuc nanoparticles (size, approximately 236.56 nm) had a spherical, core-shell structure and that they could effectively load Cur with an embedding efficiency of >95%; moreover, the system had long-term dispersion stability.
33340634	6	22	contain	had	1029:1031	arg2	structure					1057:1065	a spherical, core-shell structure	1033:1065	a spherical, core-shell structure	1033:1065	Our results showed that the Spi-Fuc nanoparticles (size, approximately 236.56 nm) had a spherical, core-shell structure and that they could effectively load Cur with an embedding efficiency of >95%; moreover, the system had long-term dispersion stability.
33340634	4	23	theme	nanoparticles	685:697	arg1	structure					660:668	The structure	656:668	The structure of the Spi-Fuc nanoparticles	656:697	The structure of the Spi-Fuc nanoparticles was studied via Fourier-transform infrared spectroscopy, transmission electron microscopy, and scanning electron microscopy.
33340634	1	24	theme	light	282:286	arg1	sensitivity					288:298	light sensitivity	282:298	light sensitivity	282:298	Curcumin (Cur) is a natural polyphenol with beneficial biological and pharmacological activities; however, it has limited applications owing to its low solubility and light sensitivity.
33340634	1	25	contain	has	225:227	arg2	applications					237:248	limited applications	229:248	limited applications owing to its low solubility and light sensitivity	229:298	Curcumin (Cur) is a natural polyphenol with beneficial biological and pharmacological activities; however, it has limited applications owing to its low solubility and light sensitivity.
33340634	1	25	contain	has	225:227	arg1	it					222:223	it	222:223	it	222:223	Curcumin (Cur) is a natural polyphenol with beneficial biological and pharmacological activities; however, it has limited applications owing to its low solubility and light sensitivity.
33340634	2	26	theme	protein-polysaccharide	305:326	arg1	complex					328:334	The protein-polysaccharide complex	301:334	The protein-polysaccharide complex	301:334	The protein-polysaccharide complex can effectively embed lipid-soluble drugs to increase their stability and dispensability in aqueous solutions.
33340634	7	27	theme	lipid-soluble	1304:1316	arg1	ingredients					1325:1335	lipid-soluble active ingredients	1304:1335	lipid-soluble active ingredients	1304:1335	Thus, we provide a simple method for Cur delivery, which can also be potentially used for delivering lipid-soluble active ingredients.
33340634	5	28	theme	nanoparticles	871:883	arg1	efficacy					855:862	the efficacy	851:862	the efficacy of the nanoparticles based on stability, drug loading rate, and simulated release	851:944	Concurrently, we evaluated the efficacy of the nanoparticles based on stability, drug loading rate, and simulated release.
33340634	1	29	theme	beneficial	159:168	arg1	activities					201:210	beneficial biological and pharmacological activities	159:210	beneficial biological and pharmacological activities	159:210	Curcumin (Cur) is a natural polyphenol with beneficial biological and pharmacological activities; however, it has limited applications owing to its low solubility and light sensitivity.
33340634	7	30	theme	active	1318:1323	arg1	ingredients					1325:1335	lipid-soluble active ingredients	1304:1335	lipid-soluble active ingredients	1304:1335	Thus, we provide a simple method for Cur delivery, which can also be potentially used for delivering lipid-soluble active ingredients.
33340634	0	31	theme	protein	44:50	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Development and characterization of soybean protein isolate and fucoidan nanoparticles for curcumin encapsulation.
33340634	0	31	theme	protein	44:50	arg1	Development					0:10	Development	0:10	Development	0:10	Development and characterization of soybean protein isolate and fucoidan nanoparticles for curcumin encapsulation.
33340634	6	32	with	load	1099:1102	arg1	efficiency					1126:1135	an embedding efficiency	1113:1135	an embedding efficiency of >95%	1113:1143	Our results showed that the Spi-Fuc nanoparticles (size, approximately 236.56 nm) had a spherical, core-shell structure and that they could effectively load Cur with an embedding efficiency of >95%; moreover, the system had long-term dispersion stability.
33340634	2	33	theme	aqueous	428:434	arg1	solutions					436:444	aqueous solutions	428:444	aqueous solutions	428:444	The protein-polysaccharide complex can effectively embed lipid-soluble drugs to increase their stability and dispensability in aqueous solutions.
33340634	0	34	theme	soybean	36:42	arg1	protein					44:50	soybean protein isolate and fucoidan	36:71	protein	44:50	Development and characterization of soybean protein isolate and fucoidan nanoparticles for curcumin encapsulation.
33340634	2	35	from	dispensability	410:423	arg1	solutions					436:444	aqueous solutions	428:444	aqueous solutions	428:444	The protein-polysaccharide complex can effectively embed lipid-soluble drugs to increase their stability and dispensability in aqueous solutions.
33340634	6	36	dep	nanoparticles	983:995	arg1	size					998:1001	size	998:1001	size	998:1001	Our results showed that the Spi-Fuc nanoparticles (size, approximately 236.56 nm) had a spherical, core-shell structure and that they could effectively load Cur with an embedding efficiency of >95%; moreover, the system had long-term dispersion stability.
33340634	6	36	dep	nanoparticles	983:995	arg1	236.56 nm					1018:1026	236.56 nm	1018:1026	236.56 nm	1018:1026	Our results showed that the Spi-Fuc nanoparticles (size, approximately 236.56 nm) had a spherical, core-shell structure and that they could effectively load Cur with an embedding efficiency of >95%; moreover, the system had long-term dispersion stability.
33340634	0	37	dep	protein	44:50	arg1	isolate					52:58	isolate	52:58	isolate	52:58	Development and characterization of soybean protein isolate and fucoidan nanoparticles for curcumin encapsulation.
33340634	6	38	theme	Spi-Fuc	975:981	arg1	nanoparticles					983:995	the Spi-Fuc nanoparticles	971:995	the Spi-Fuc nanoparticles (size, approximately 236.56 nm)	971:1027	Our results showed that the Spi-Fuc nanoparticles (size, approximately 236.56 nm) had a spherical, core-shell structure and that they could effectively load Cur with an embedding efficiency of >95%; moreover, the system had long-term dispersion stability.
33340634	3	39	used	used	501:504	arg2	Fuc					491:493	Fuc	491:493	Fuc	491:493	Soybean protein isolate (Spi) and fucoidan (Fuc) were used as a polymer matrix, and core-shell nanoparticles were prepared to encapsulate Cur via electrostatic interaction under acidic and neutral conditions.
33340634	3	39	used	used	501:504	arg2	isolate					463:469	Soybean protein isolate	447:469	Soybean protein isolate (Spi)	447:475	Soybean protein isolate (Spi) and fucoidan (Fuc) were used as a polymer matrix, and core-shell nanoparticles were prepared to encapsulate Cur via electrostatic interaction under acidic and neutral conditions.
33340634	3	39	used	used	501:504	arg2	fucoidan					481:488	fucoidan	481:488	fucoidan (Fuc)	481:494	Soybean protein isolate (Spi) and fucoidan (Fuc) were used as a polymer matrix, and core-shell nanoparticles were prepared to encapsulate Cur via electrostatic interaction under acidic and neutral conditions.
33340634	3	39	used	used	501:504	arg2	matrix					519:524	a polymer matrix	509:524	a polymer matrix	509:524	Soybean protein isolate (Spi) and fucoidan (Fuc) were used as a polymer matrix, and core-shell nanoparticles were prepared to encapsulate Cur via electrostatic interaction under acidic and neutral conditions.
33340634	3	39	used	used	501:504	arg2	Spi					472:474	Spi	472:474	Spi	472:474	Soybean protein isolate (Spi) and fucoidan (Fuc) were used as a polymer matrix, and core-shell nanoparticles were prepared to encapsulate Cur via electrostatic interaction under acidic and neutral conditions.
33340634	2	40	theme	lipid-soluble	358:370	arg1	drugs					372:376	lipid-soluble drugs	358:376	lipid-soluble drugs	358:376	The protein-polysaccharide complex can effectively embed lipid-soluble drugs to increase their stability and dispensability in aqueous solutions.
33340634	3	41	theme	neutral	636:642	arg1	conditions					644:653	acidic and neutral conditions	625:653	acidic and neutral conditions	625:653	Soybean protein isolate (Spi) and fucoidan (Fuc) were used as a polymer matrix, and core-shell nanoparticles were prepared to encapsulate Cur via electrostatic interaction under acidic and neutral conditions.
33340634	4	42	theme	electron	769:776	arg1	microscopy					778:787	transmission electron microscopy	756:787	transmission electron microscopy	756:787	The structure of the Spi-Fuc nanoparticles was studied via Fourier-transform infrared spectroscopy, transmission electron microscopy, and scanning electron microscopy.
33340634	4	43	theme	scanning	794:801	arg1	microscopy					812:821	scanning electron microscopy	794:821	scanning electron microscopy	794:821	The structure of the Spi-Fuc nanoparticles was studied via Fourier-transform infrared spectroscopy, transmission electron microscopy, and scanning electron microscopy.
33340634	6	44	theme	dispersion	1181:1190	arg1	stability					1192:1200	long-term dispersion stability	1171:1200	long-term dispersion stability	1171:1200	Our results showed that the Spi-Fuc nanoparticles (size, approximately 236.56 nm) had a spherical, core-shell structure and that they could effectively load Cur with an embedding efficiency of >95%; moreover, the system had long-term dispersion stability.
33340634	4	45	theme	transmission	756:767	arg1	microscopy					778:787	transmission electron microscopy	756:787	transmission electron microscopy	756:787	The structure of the Spi-Fuc nanoparticles was studied via Fourier-transform infrared spectroscopy, transmission electron microscopy, and scanning electron microscopy.
33340634	1	46	theme	limited	229:235	arg1	applications					237:248	limited applications	229:248	limited applications owing to its low solubility and light sensitivity	229:298	Curcumin (Cur) is a natural polyphenol with beneficial biological and pharmacological activities; however, it has limited applications owing to its low solubility and light sensitivity.
33340634	3	47	theme	acidic	625:630	arg1	conditions					644:653	acidic and neutral conditions	625:653	acidic and neutral conditions	625:653	Soybean protein isolate (Spi) and fucoidan (Fuc) were used as a polymer matrix, and core-shell nanoparticles were prepared to encapsulate Cur via electrostatic interaction under acidic and neutral conditions.
33340634	1	48	theme	natural	135:141	arg1	polyphenol					143:152	a natural polyphenol	133:152	a natural polyphenol with beneficial biological and pharmacological activities	133:210	Curcumin (Cur) is a natural polyphenol with beneficial biological and pharmacological activities; however, it has limited applications owing to its low solubility and light sensitivity.
33340634	1	48	theme	natural	135:141	arg1	Curcumin					115:122	Curcumin	115:122	Curcumin (Cur)	115:128	Curcumin (Cur) is a natural polyphenol with beneficial biological and pharmacological activities; however, it has limited applications owing to its low solubility and light sensitivity.
33340634	7	49	theme	Cur	1240:1242	arg1	delivery					1244:1251	Cur delivery	1240:1251	Cur delivery	1240:1251	Thus, we provide a simple method for Cur delivery, which can also be potentially used for delivering lipid-soluble active ingredients.
33340634	0	50	theme	fucoidan	64:71	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Development and characterization of soybean protein isolate and fucoidan nanoparticles for curcumin encapsulation.
33340634	0	50	theme	fucoidan	64:71	arg1	Development					0:10	Development	0:10	Development	0:10	Development and characterization of soybean protein isolate and fucoidan nanoparticles for curcumin encapsulation.
33340634	3	51	theme	protein	455:461	arg1	Spi					472:474	Spi	472:474	Spi	472:474	Soybean protein isolate (Spi) and fucoidan (Fuc) were used as a polymer matrix, and core-shell nanoparticles were prepared to encapsulate Cur via electrostatic interaction under acidic and neutral conditions.
33340634	3	51	theme	protein	455:461	arg1	fucoidan					481:488	fucoidan	481:488	fucoidan (Fuc)	481:494	Soybean protein isolate (Spi) and fucoidan (Fuc) were used as a polymer matrix, and core-shell nanoparticles were prepared to encapsulate Cur via electrostatic interaction under acidic and neutral conditions.
33340634	3	51	theme	protein	455:461	arg1	isolate					463:469	Soybean protein isolate	447:469	Soybean protein isolate (Spi)	447:475	Soybean protein isolate (Spi) and fucoidan (Fuc) were used as a polymer matrix, and core-shell nanoparticles were prepared to encapsulate Cur via electrostatic interaction under acidic and neutral conditions.
33340634	3	51	theme	protein	455:461	arg1	matrix					519:524	a polymer matrix	509:524	a polymer matrix	509:524	Soybean protein isolate (Spi) and fucoidan (Fuc) were used as a polymer matrix, and core-shell nanoparticles were prepared to encapsulate Cur via electrostatic interaction under acidic and neutral conditions.
33340634	6	52	theme	%	1143:1143	arg1	efficiency					1126:1135	an embedding efficiency	1113:1135	an embedding efficiency of >95%	1113:1143	Our results showed that the Spi-Fuc nanoparticles (size, approximately 236.56 nm) had a spherical, core-shell structure and that they could effectively load Cur with an embedding efficiency of >95%; moreover, the system had long-term dispersion stability.
33340634	5	53	theme	simulated	928:936	arg1	release					938:944	simulated release	928:944	simulated release	928:944	Concurrently, we evaluated the efficacy of the nanoparticles based on stability, drug loading rate, and simulated release.
33340634	6	54	contain	had	1167:1169	arg2	stability					1192:1200	long-term dispersion stability	1171:1200	long-term dispersion stability	1171:1200	Our results showed that the Spi-Fuc nanoparticles (size, approximately 236.56 nm) had a spherical, core-shell structure and that they could effectively load Cur with an embedding efficiency of >95%; moreover, the system had long-term dispersion stability.
33340634	6	54	contain	had	1167:1169	arg1	system					1160:1165	the system	1156:1165	the system	1156:1165	Our results showed that the Spi-Fuc nanoparticles (size, approximately 236.56 nm) had a spherical, core-shell structure and that they could effectively load Cur with an embedding efficiency of >95%; moreover, the system had long-term dispersion stability.
34780741	8	0	theme	self-protecting	1678:1692	arg1	materials					1694:1702	the essential self-protecting materials	1664:1702	the essential self-protecting materials	1664:1702	These results also implied that, as the essential self-protecting materials, more EPSs with higher proportion of CPS would be excreted to response the low N environment.
34780741	8	0	theme	self-protecting	1678:1692	arg1	EPSs					1710:1713	more EPSs	1705:1713	more EPSs with higher proportion of CPS	1705:1743	These results also implied that, as the essential self-protecting materials, more EPSs with higher proportion of CPS would be excreted to response the low N environment.
34780741	8	1	theme	more	1705:1708	arg1	materials					1694:1702	the essential self-protecting materials	1664:1702	the essential self-protecting materials	1664:1702	These results also implied that, as the essential self-protecting materials, more EPSs with higher proportion of CPS would be excreted to response the low N environment.
34780741	8	1	theme	more	1705:1708	arg1	EPSs					1710:1713	more EPSs	1705:1713	more EPSs with higher proportion of CPS	1705:1743	These results also implied that, as the essential self-protecting materials, more EPSs with higher proportion of CPS would be excreted to response the low N environment.
34780741	1	2	theme	soil	146:149	arg1	BSCs					159:162	BSCs	159:162	BSCs	159:162	As an important carbon (C) storage in biological soil crusts (BSCs), exopolysaccharides (EPSs) are not only a part of the desert C cycle, but also the key materials for cyanobacteria to resist desert stress.
34780741	1	2	theme	soil	146:149	arg1	crusts					151:156	biological soil crusts	135:156	biological soil crusts (BSCs)	135:163	As an important carbon (C) storage in biological soil crusts (BSCs), exopolysaccharides (EPSs) are not only a part of the desert C cycle, but also the key materials for cyanobacteria to resist desert stress.
34780741	5	3	theme	monosaccharidic	1072:1086	arg1	composition					1088:1098	monosaccharidic composition	1072:1098	monosaccharidic composition	1072:1098	Notably, obvious difference displayed in the monosaccharidic composition and morphologies between CPS and RPS, but not the N levels.
34780741	7	4	theme	excretion	1548:1556	arg1	pattern					1558:1564	excretion pattern	1548:1564	excretion pattern	1548:1564	Our results showed that, as the raw material and environmental signal, environmental N concentration regulates the elements (C and N) percentage of cyanobacterial cells and its EPSs excretion pattern, but not the monosaccharidic composition or the morphologies.
34780741	3	5	theme	highest	735:741	arg1	concentration					745:757	the highest N concentration	731:757	the highest N concentration (about 40 mg L-1) in the medium	731:789	In logarithmic period, higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs was observed in the N50 group with the highest N concentration (about 40 mg L-1) in the medium, while no difference was observed among the three groups in stationary period when the N concentrations of medium were lower than 25 mg L-1.
34780741	6	6	theme	C/N	1175:1177	arg1	changes					1164:1170	The changes	1160:1170	The changes of C/N in cells at different growth period	1160:1213	The changes of C/N in cells at different growth period indicate that the excretion of EPSs, a mechanism that maintains the balance of cell C/N ratio, only works when the N in the environment is sufficient.
34780741	7	7	dep	elements	1481:1488	arg1	N					1497:1497	N	1497:1497	N	1497:1497	Our results showed that, as the raw material and environmental signal, environmental N concentration regulates the elements (C and N) percentage of cyanobacterial cells and its EPSs excretion pattern, but not the monosaccharidic composition or the morphologies.
34780741	7	7	dep	elements	1481:1488	arg1	C					1491:1491	C	1491:1491	C	1491:1491	Our results showed that, as the raw material and environmental signal, environmental N concentration regulates the elements (C and N) percentage of cyanobacterial cells and its EPSs excretion pattern, but not the monosaccharidic composition or the morphologies.
34780741	7	7	dep	elements	1481:1488	arg1	elements					1481:1488	the elements	1477:1488	the elements (C and N)	1477:1498	Our results showed that, as the raw material and environmental signal, environmental N concentration regulates the elements (C and N) percentage of cyanobacterial cells and its EPSs excretion pattern, but not the monosaccharidic composition or the morphologies.
34780741	7	7	dep	elements	1481:1488	arg1	percentage					1500:1509	percentage	1500:1509	percentage of cyanobacterial cells and its EPSs excretion pattern, but not the monosaccharidic composition	1500:1605	Our results showed that, as the raw material and environmental signal, environmental N concentration regulates the elements (C and N) percentage of cyanobacterial cells and its EPSs excretion pattern, but not the monosaccharidic composition or the morphologies.
34780741	3	8	located	observed	700:707	arg1	period					629:634	logarithmic period	617:634	logarithmic period	617:634	In logarithmic period, higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs was observed in the N50 group with the highest N concentration (about 40 mg L-1) in the medium, while no difference was observed among the three groups in stationary period when the N concentrations of medium were lower than 25 mg L-1.
34780741	3	8	located	observed	700:707	arg1	group					720:724	the N50 group	712:724	the N50 group with the highest N concentration (about 40 mg L-1) in the medium	712:789	In logarithmic period, higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs was observed in the N50 group with the highest N concentration (about 40 mg L-1) in the medium, while no difference was observed among the three groups in stationary period when the N concentrations of medium were lower than 25 mg L-1.
34780741	3	8	located	observed	700:707	arg2	ratio					644:648	higher ratio	637:648	higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs	637:694	In logarithmic period, higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs was observed in the N50 group with the highest N concentration (about 40 mg L-1) in the medium, while no difference was observed among the three groups in stationary period when the N concentrations of medium were lower than 25 mg L-1.
34780741	2	9	theme	capsule	542:548	arg1	CPS					537:539	CPS	537:539	CPS (capsule exopolysaccharides)	537:568	In this study, the influence of initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively) on Microcoleus vaginatusis growth and the excretion of EPSs including RPS (released exopolysaccharides) and CPS (capsule exopolysaccharides) were evaluated at different growth periods.
34780741	2	9	theme	capsule	542:548	arg1	exopolysaccharides					550:567	capsule exopolysaccharides	542:567	capsule exopolysaccharides	542:567	In this study, the influence of initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively) on Microcoleus vaginatusis growth and the excretion of EPSs including RPS (released exopolysaccharides) and CPS (capsule exopolysaccharides) were evaluated at different growth periods.
34780741	3	10	theme	N	743:743	arg1	concentration					745:757	the highest N concentration	731:757	the highest N concentration (about 40 mg L-1) in the medium	731:789	In logarithmic period, higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs was observed in the N50 group with the highest N concentration (about 40 mg L-1) in the medium, while no difference was observed among the three groups in stationary period when the N concentrations of medium were lower than 25 mg L-1.
34780741	8	11	theme	CPS	1741:1743	arg1	proportion					1727:1736	higher proportion	1720:1736	higher proportion of CPS	1720:1743	These results also implied that, as the essential self-protecting materials, more EPSs with higher proportion of CPS would be excreted to response the low N environment.
34780741	1	12	theme	desert	290:295	arg1	stress					297:302	desert stress	290:302	desert stress	290:302	As an important carbon (C) storage in biological soil crusts (BSCs), exopolysaccharides (EPSs) are not only a part of the desert C cycle, but also the key materials for cyanobacteria to resist desert stress.
34780741	2	13	theme	EPSs	484:487	arg1	excretion					471:479	the excretion	467:479	the excretion of EPSs including RPS (released exopolysaccharides) and CPS (capsule exopolysaccharides)	467:568	In this study, the influence of initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively) on Microcoleus vaginatusis growth and the excretion of EPSs including RPS (released exopolysaccharides) and CPS (capsule exopolysaccharides) were evaluated at different growth periods.
34780741	2	13	theme	EPSs	484:487	arg1	influence					324:332	the influence	320:332	the influence of initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively) on Microcoleus vaginatusis growth	320:461	In this study, the influence of initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively) on Microcoleus vaginatusis growth and the excretion of EPSs including RPS (released exopolysaccharides) and CPS (capsule exopolysaccharides) were evaluated at different growth periods.
34780741	7	14	theme	monosaccharidic	1579:1593	arg1	composition					1595:1605	the monosaccharidic composition	1575:1605	the monosaccharidic composition	1575:1605	Our results showed that, as the raw material and environmental signal, environmental N concentration regulates the elements (C and N) percentage of cyanobacterial cells and its EPSs excretion pattern, but not the monosaccharidic composition or the morphologies.
34780741	4	15	theme	DW-CPS	973:978	arg1	/EPSs					980:984	(DW-CPS)/EPSs	972:984	(DW-CPS)/EPSs	972:984	The CPS/RPS showed similar results with (DW-CPS)/EPSs, and stayed higher than 1 in each group.
34780741	1	16	theme	desert	219:224	arg1	cycle					228:232	the desert C cycle	215:232	the desert C cycle	215:232	As an important carbon (C) storage in biological soil crusts (BSCs), exopolysaccharides (EPSs) are not only a part of the desert C cycle, but also the key materials for cyanobacteria to resist desert stress.
34780741	3	17	with	group	720:724	arg1	concentration					745:757	the highest N concentration	731:757	the highest N concentration (about 40 mg L-1) in the medium	731:789	In logarithmic period, higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs was observed in the N50 group with the highest N concentration (about 40 mg L-1) in the medium, while no difference was observed among the three groups in stationary period when the N concentrations of medium were lower than 25 mg L-1.
34780741	3	18	theme	stationary	851:860	arg1	period					862:867	stationary period	851:867	stationary period	851:867	In logarithmic period, higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs was observed in the N50 group with the highest N concentration (about 40 mg L-1) in the medium, while no difference was observed among the three groups in stationary period when the N concentrations of medium were lower than 25 mg L-1.
34780741	3	19	from	concentration	745:757	arg1	medium					784:789	the medium	780:789	the medium	780:789	In logarithmic period, higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs was observed in the N50 group with the highest N concentration (about 40 mg L-1) in the medium, while no difference was observed among the three groups in stationary period when the N concentrations of medium were lower than 25 mg L-1.
34780741	5	20	theme	N	1150:1150	arg1	levels					1152:1157	the N levels	1146:1157	the N levels	1146:1157	Notably, obvious difference displayed in the monosaccharidic composition and morphologies between CPS and RPS, but not the N levels.
34780741	2	21	from	excretion	471:479	arg1	growth					456:461	Microcoleus vaginatusis growth	432:461	Microcoleus vaginatusis growth	432:461	In this study, the influence of initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively) on Microcoleus vaginatusis growth and the excretion of EPSs including RPS (released exopolysaccharides) and CPS (capsule exopolysaccharides) were evaluated at different growth periods.
34780741	2	22	dep	concentrations	347:360	arg1	10					363:364	10	363:364	10	363:364	In this study, the influence of initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively) on Microcoleus vaginatusis growth and the excretion of EPSs including RPS (released exopolysaccharides) and CPS (capsule exopolysaccharides) were evaluated at different growth periods.
34780741	2	22	dep	concentrations	347:360	arg1	50 mg L-1					374:382	50 mg L-1	374:382	50 mg L-1	374:382	In this study, the influence of initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively) on Microcoleus vaginatusis growth and the excretion of EPSs including RPS (released exopolysaccharides) and CPS (capsule exopolysaccharides) were evaluated at different growth periods.
34780741	5	23	dep	composition	1088:1098	arg1	the					1068:1070	the	1068:1070	the	1068:1070	Notably, obvious difference displayed in the monosaccharidic composition and morphologies between CPS and RPS, but not the N levels.
34780741	7	24	theme	EPSs	1543:1546	arg1	percentage					1500:1509	percentage	1500:1509	percentage of cyanobacterial cells and its EPSs excretion pattern, but not the monosaccharidic composition	1500:1605	Our results showed that, as the raw material and environmental signal, environmental N concentration regulates the elements (C and N) percentage of cyanobacterial cells and its EPSs excretion pattern, but not the monosaccharidic composition or the morphologies.
34780741	6	25	theme	EPSs	1246:1249	arg1	excretion					1233:1241	the excretion	1229:1241	the excretion of EPSs, a mechanism that maintains the balance of cell C/N ratio,	1229:1308	The changes of C/N in cells at different growth period indicate that the excretion of EPSs, a mechanism that maintains the balance of cell C/N ratio, only works when the N in the environment is sufficient.
34780741	3	26	theme	logarithmic	617:627	arg1	period					629:634	logarithmic period	617:634	logarithmic period	617:634	In logarithmic period, higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs was observed in the N50 group with the highest N concentration (about 40 mg L-1) in the medium, while no difference was observed among the three groups in stationary period when the N concentrations of medium were lower than 25 mg L-1.
34780741	1	27	from	storage	124:130	arg1	BSCs					159:162	BSCs	159:162	BSCs	159:162	As an important carbon (C) storage in biological soil crusts (BSCs), exopolysaccharides (EPSs) are not only a part of the desert C cycle, but also the key materials for cyanobacteria to resist desert stress.
34780741	1	27	from	storage	124:130	arg1	crusts					151:156	biological soil crusts	135:156	biological soil crusts (BSCs)	135:163	As an important carbon (C) storage in biological soil crusts (BSCs), exopolysaccharides (EPSs) are not only a part of the desert C cycle, but also the key materials for cyanobacteria to resist desert stress.
34780741	7	28	theme	N	1451:1451	arg1	concentration					1453:1465	environmental N concentration	1437:1465	environmental N concentration	1437:1465	Our results showed that, as the raw material and environmental signal, environmental N concentration regulates the elements (C and N) percentage of cyanobacterial cells and its EPSs excretion pattern, but not the monosaccharidic composition or the morphologies.
34780741	0	29	theme	Nitrogen	0:7	arg1	concentration					9:21	Nitrogen concentration	0:21	Nitrogen concentration acting as an environmental signal	0:55	Nitrogen concentration acting as an environmental signal regulates cyanobacterial EPS excretion.
34780741	1	30	theme	C	226:226	arg1	cycle					228:232	the desert C cycle	215:232	the desert C cycle	215:232	As an important carbon (C) storage in biological soil crusts (BSCs), exopolysaccharides (EPSs) are not only a part of the desert C cycle, but also the key materials for cyanobacteria to resist desert stress.
34780741	2	31	theme	released	504:511	arg1	RPS					499:501	RPS	499:501	RPS (released exopolysaccharides)	499:531	In this study, the influence of initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively) on Microcoleus vaginatusis growth and the excretion of EPSs including RPS (released exopolysaccharides) and CPS (capsule exopolysaccharides) were evaluated at different growth periods.
34780741	2	31	theme	released	504:511	arg1	exopolysaccharides					513:530	released exopolysaccharides	504:530	released exopolysaccharides	504:530	In this study, the influence of initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively) on Microcoleus vaginatusis growth and the excretion of EPSs including RPS (released exopolysaccharides) and CPS (capsule exopolysaccharides) were evaluated at different growth periods.
34780741	3	32	dep	concentration	745:757	arg1	40 mg L-1					766:774	40 mg L-1	766:774	40 mg L-1	766:774	In logarithmic period, higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs was observed in the N50 group with the highest N concentration (about 40 mg L-1) in the medium, while no difference was observed among the three groups in stationary period when the N concentrations of medium were lower than 25 mg L-1.
34780741	4	33	theme	similar	951:957	arg1	results					959:965	similar results	951:965	similar results	951:965	The CPS/RPS showed similar results with (DW-CPS)/EPSs, and stayed higher than 1 in each group.
34780741	8	34	theme	low	1779:1781	arg1	environment					1785:1795	the low N environment	1775:1795	the low N environment	1775:1795	These results also implied that, as the essential self-protecting materials, more EPSs with higher proportion of CPS would be excreted to response the low N environment.
34780741	1	35	theme	cycle	228:232	arg1	exopolysaccharides					166:183	exopolysaccharides	166:183	exopolysaccharides (EPSs)	166:190	As an important carbon (C) storage in biological soil crusts (BSCs), exopolysaccharides (EPSs) are not only a part of the desert C cycle, but also the key materials for cyanobacteria to resist desert stress.
34780741	1	35	theme	cycle	228:232	arg1	part					207:210	a part	205:210	a part of the desert C cycle	205:232	As an important carbon (C) storage in biological soil crusts (BSCs), exopolysaccharides (EPSs) are not only a part of the desert C cycle, but also the key materials for cyanobacteria to resist desert stress.
34780741	1	35	theme	cycle	228:232	arg1	materials					252:260	the key materials	244:260	the key materials for cyanobacteria	244:278	As an important carbon (C) storage in biological soil crusts (BSCs), exopolysaccharides (EPSs) are not only a part of the desert C cycle, but also the key materials for cyanobacteria to resist desert stress.
34780741	3	36	theme	N	878:878	arg1	lower					910:914	lower	910:914	lower	910:914	In logarithmic period, higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs was observed in the N50 group with the highest N concentration (about 40 mg L-1) in the medium, while no difference was observed among the three groups in stationary period when the N concentrations of medium were lower than 25 mg L-1.
34780741	3	36	theme	N	878:878	arg1	concentrations					880:893	the N concentrations	874:893	the N concentrations of medium	874:903	In logarithmic period, higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs was observed in the N50 group with the highest N concentration (about 40 mg L-1) in the medium, while no difference was observed among the three groups in stationary period when the N concentrations of medium were lower than 25 mg L-1.
34780741	1	37	theme	important	103:111	arg1	storage					124:130	an important carbon (C) storage	100:130	an important carbon (C) storage in biological soil crusts (BSCs)	100:163	As an important carbon (C) storage in biological soil crusts (BSCs), exopolysaccharides (EPSs) are not only a part of the desert C cycle, but also the key materials for cyanobacteria to resist desert stress.
34780741	6	38	theme	ratio	1303:1307	arg1	balance					1283:1289	the balance	1279:1289	the balance of cell C/N ratio	1279:1307	The changes of C/N in cells at different growth period indicate that the excretion of EPSs, a mechanism that maintains the balance of cell C/N ratio, only works when the N in the environment is sufficient.
34780741	2	39	theme	N	345:345	arg1	concentrations					347:360	initial N concentrations	337:360	initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively)	337:427	In this study, the influence of initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively) on Microcoleus vaginatusis growth and the excretion of EPSs including RPS (released exopolysaccharides) and CPS (capsule exopolysaccharides) were evaluated at different growth periods.
34780741	2	40	theme	concentrations	347:360	arg1	excretion					471:479	the excretion	467:479	the excretion of EPSs including RPS (released exopolysaccharides) and CPS (capsule exopolysaccharides)	467:568	In this study, the influence of initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively) on Microcoleus vaginatusis growth and the excretion of EPSs including RPS (released exopolysaccharides) and CPS (capsule exopolysaccharides) were evaluated at different growth periods.
34780741	2	40	theme	concentrations	347:360	arg1	influence					324:332	the influence	320:332	the influence of initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively) on Microcoleus vaginatusis growth	320:461	In this study, the influence of initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively) on Microcoleus vaginatusis growth and the excretion of EPSs including RPS (released exopolysaccharides) and CPS (capsule exopolysaccharides) were evaluated at different growth periods.
34780741	3	41	theme	higher	637:642	arg1	ratio					644:648	higher ratio	637:648	higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs	637:694	In logarithmic period, higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs was observed in the N50 group with the highest N concentration (about 40 mg L-1) in the medium, while no difference was observed among the three groups in stationary period when the N concentrations of medium were lower than 25 mg L-1.
34780741	2	42	theme	vaginatusis	444:454	arg1	growth					456:461	Microcoleus vaginatusis growth	432:461	Microcoleus vaginatusis growth	432:461	In this study, the influence of initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively) on Microcoleus vaginatusis growth and the excretion of EPSs including RPS (released exopolysaccharides) and CPS (capsule exopolysaccharides) were evaluated at different growth periods.
34780741	1	43	theme	carbon	113:118	arg1	storage					124:130	an important carbon (C) storage	100:130	an important carbon (C) storage in biological soil crusts (BSCs)	100:163	As an important carbon (C) storage in biological soil crusts (BSCs), exopolysaccharides (EPSs) are not only a part of the desert C cycle, but also the key materials for cyanobacteria to resist desert stress.
34780741	6	44	theme	C/N	1299:1301	arg1	ratio					1303:1307	cell C/N ratio	1294:1307	cell C/N ratio	1294:1307	The changes of C/N in cells at different growth period indicate that the excretion of EPSs, a mechanism that maintains the balance of cell C/N ratio, only works when the N in the environment is sufficient.
34780741	2	45	theme	initial	337:343	arg1	concentrations					347:360	initial N concentrations	337:360	initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively)	337:427	In this study, the influence of initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively) on Microcoleus vaginatusis growth and the excretion of EPSs including RPS (released exopolysaccharides) and CPS (capsule exopolysaccharides) were evaluated at different growth periods.
34780741	0	46	theme	environmental	36:48	arg1	signal					50:55	an environmental signal	33:55	an environmental signal	33:55	Nitrogen concentration acting as an environmental signal regulates cyanobacterial EPS excretion.
34780741	2	47	theme	Microcoleus	432:442	arg1	growth					456:461	Microcoleus vaginatusis growth	432:461	Microcoleus vaginatusis growth	432:461	In this study, the influence of initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively) on Microcoleus vaginatusis growth and the excretion of EPSs including RPS (released exopolysaccharides) and CPS (capsule exopolysaccharides) were evaluated at different growth periods.
34780741	6	48	theme	cell	1294:1297	arg1	ratio					1303:1307	cell C/N ratio	1294:1307	cell C/N ratio	1294:1307	The changes of C/N in cells at different growth period indicate that the excretion of EPSs, a mechanism that maintains the balance of cell C/N ratio, only works when the N in the environment is sufficient.
34780741	8	49	theme	N	1783:1783	arg1	environment					1785:1795	the low N environment	1775:1795	the low N environment	1775:1795	These results also implied that, as the essential self-protecting materials, more EPSs with higher proportion of CPS would be excreted to response the low N environment.
34780741	7	50	theme	environmental	1415:1427	arg1	signal					1429:1434	environmental signal	1415:1434	environmental signal	1415:1434	Our results showed that, as the raw material and environmental signal, environmental N concentration regulates the elements (C and N) percentage of cyanobacterial cells and its EPSs excretion pattern, but not the monosaccharidic composition or the morphologies.
34780741	3	51	theme	medium	898:903	arg1	lower					910:914	lower	910:914	lower	910:914	In logarithmic period, higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs was observed in the N50 group with the highest N concentration (about 40 mg L-1) in the medium, while no difference was observed among the three groups in stationary period when the N concentrations of medium were lower than 25 mg L-1.
34780741	3	51	theme	medium	898:903	arg1	concentrations					880:893	the N concentrations	874:893	the N concentrations of medium	874:903	In logarithmic period, higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs was observed in the N50 group with the highest N concentration (about 40 mg L-1) in the medium, while no difference was observed among the three groups in stationary period when the N concentrations of medium were lower than 25 mg L-1.
34780741	3	52	theme	biomass	653:659	arg1	ratio					644:648	higher ratio	637:648	higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs	637:694	In logarithmic period, higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs was observed in the N50 group with the highest N concentration (about 40 mg L-1) in the medium, while no difference was observed among the three groups in stationary period when the N concentrations of medium were lower than 25 mg L-1.
34780741	8	53	theme	higher	1720:1725	arg1	proportion					1727:1736	higher proportion	1720:1736	higher proportion of CPS	1720:1743	These results also implied that, as the essential self-protecting materials, more EPSs with higher proportion of CPS would be excreted to response the low N environment.
34780741	6	54	theme	growth	1201:1206	arg1	period					1208:1213	different growth period	1191:1213	different growth period	1191:1213	The changes of C/N in cells at different growth period indicate that the excretion of EPSs, a mechanism that maintains the balance of cell C/N ratio, only works when the N in the environment is sufficient.
34780741	1	55	theme	key	248:250	arg1	materials					252:260	the key materials	244:260	the key materials for cyanobacteria	244:278	As an important carbon (C) storage in biological soil crusts (BSCs), exopolysaccharides (EPSs) are not only a part of the desert C cycle, but also the key materials for cyanobacteria to resist desert stress.
34780741	8	56	theme	essential	1668:1676	arg1	materials					1694:1702	the essential self-protecting materials	1664:1702	the essential self-protecting materials	1664:1702	These results also implied that, as the essential self-protecting materials, more EPSs with higher proportion of CPS would be excreted to response the low N environment.
34780741	8	56	theme	essential	1668:1676	arg1	EPSs					1710:1713	more EPSs	1705:1713	more EPSs with higher proportion of CPS	1705:1743	These results also implied that, as the essential self-protecting materials, more EPSs with higher proportion of CPS would be excreted to response the low N environment.
34780741	6	57	from	period	1208:1213	arg1	cells					1182:1186	cells	1182:1186	cells at different growth period	1182:1213	The changes of C/N in cells at different growth period indicate that the excretion of EPSs, a mechanism that maintains the balance of cell C/N ratio, only works when the N in the environment is sufficient.
34780741	6	57	from	period	1208:1213	arg1	changes					1164:1170	The changes	1160:1170	The changes of C/N in cells at different growth period	1160:1213	The changes of C/N in cells at different growth period indicate that the excretion of EPSs, a mechanism that maintains the balance of cell C/N ratio, only works when the N in the environment is sufficient.
34780741	2	58	theme	growth	598:603	arg1	periods					605:611	different growth periods	588:611	different growth periods	588:611	In this study, the influence of initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively) on Microcoleus vaginatusis growth and the excretion of EPSs including RPS (released exopolysaccharides) and CPS (capsule exopolysaccharides) were evaluated at different growth periods.
34780741	1	59	theme	C	121:121	arg1	storage					124:130	an important carbon (C) storage	100:130	an important carbon (C) storage in biological soil crusts (BSCs)	100:163	As an important carbon (C) storage in biological soil crusts (BSCs), exopolysaccharides (EPSs) are not only a part of the desert C cycle, but also the key materials for cyanobacteria to resist desert stress.
34780741	7	60	theme	cyanobacterial	1514:1527	arg1	cells					1529:1533	cyanobacterial cells	1514:1533	cyanobacterial cells	1514:1533	Our results showed that, as the raw material and environmental signal, environmental N concentration regulates the elements (C and N) percentage of cyanobacterial cells and its EPSs excretion pattern, but not the monosaccharidic composition or the morphologies.
34780741	7	61	theme	cells	1529:1533	arg1	percentage					1500:1509	percentage	1500:1509	percentage of cyanobacterial cells and its EPSs excretion pattern, but not the monosaccharidic composition	1500:1605	Our results showed that, as the raw material and environmental signal, environmental N concentration regulates the elements (C and N) percentage of cyanobacterial cells and its EPSs excretion pattern, but not the monosaccharidic composition or the morphologies.
34780741	2	62	theme	different	588:596	arg1	periods					605:611	different growth periods	588:611	different growth periods	588:611	In this study, the influence of initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively) on Microcoleus vaginatusis growth and the excretion of EPSs including RPS (released exopolysaccharides) and CPS (capsule exopolysaccharides) were evaluated at different growth periods.
34780741	7	63	dep	EPSs	1543:1546	arg1	composition					1595:1605	the monosaccharidic composition	1575:1605	the monosaccharidic composition	1575:1605	Our results showed that, as the raw material and environmental signal, environmental N concentration regulates the elements (C and N) percentage of cyanobacterial cells and its EPSs excretion pattern, but not the monosaccharidic composition or the morphologies.
34780741	7	63	dep	EPSs	1543:1546	arg1	pattern					1558:1564	excretion pattern	1548:1564	excretion pattern	1548:1564	Our results showed that, as the raw material and environmental signal, environmental N concentration regulates the elements (C and N) percentage of cyanobacterial cells and its EPSs excretion pattern, but not the monosaccharidic composition or the morphologies.
34780741	5	64	theme	obvious	1036:1042	arg1	difference					1044:1053	obvious difference	1036:1053	obvious difference	1036:1053	Notably, obvious difference displayed in the monosaccharidic composition and morphologies between CPS and RPS, but not the N levels.
34780741	7	65	theme	raw	1398:1400	arg1	morphologies					1614:1625	the morphologies	1610:1625	the morphologies	1610:1625	Our results showed that, as the raw material and environmental signal, environmental N concentration regulates the elements (C and N) percentage of cyanobacterial cells and its EPSs excretion pattern, but not the monosaccharidic composition or the morphologies.
34780741	7	65	theme	raw	1398:1400	arg1	elements					1481:1488	the elements	1477:1488	the elements (C and N)	1477:1498	Our results showed that, as the raw material and environmental signal, environmental N concentration regulates the elements (C and N) percentage of cyanobacterial cells and its EPSs excretion pattern, but not the monosaccharidic composition or the morphologies.
34780741	7	65	theme	raw	1398:1400	arg1	material					1402:1409	the raw material	1394:1409	the raw material	1394:1409	Our results showed that, as the raw material and environmental signal, environmental N concentration regulates the elements (C and N) percentage of cyanobacterial cells and its EPSs excretion pattern, but not the monosaccharidic composition or the morphologies.
34780741	6	66	theme	different	1191:1199	arg1	period					1208:1213	different growth period	1191:1213	different growth period	1191:1213	The changes of C/N in cells at different growth period indicate that the excretion of EPSs, a mechanism that maintains the balance of cell C/N ratio, only works when the N in the environment is sufficient.
34780741	6	67	from	changes	1164:1170	arg1	cells					1182:1186	cells	1182:1186	cells at different growth period	1182:1213	The changes of C/N in cells at different growth period indicate that the excretion of EPSs, a mechanism that maintains the balance of cell C/N ratio, only works when the N in the environment is sufficient.
34780741	6	67	from	changes	1164:1170	arg1	period					1208:1213	different growth period	1191:1213	different growth period	1191:1213	The changes of C/N in cells at different growth period indicate that the excretion of EPSs, a mechanism that maintains the balance of cell C/N ratio, only works when the N in the environment is sufficient.
34780741	6	68	from	N	1330:1330	arg1	environment					1339:1349	the environment	1335:1349	the environment	1335:1349	The changes of C/N in cells at different growth period indicate that the excretion of EPSs, a mechanism that maintains the balance of cell C/N ratio, only works when the N in the environment is sufficient.
34780741	3	69	theme	N50	716:718	arg1	group					720:724	the N50 group	712:724	the N50 group with the highest N concentration (about 40 mg L-1) in the medium	712:789	In logarithmic period, higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs was observed in the N50 group with the highest N concentration (about 40 mg L-1) in the medium, while no difference was observed among the three groups in stationary period when the N concentrations of medium were lower than 25 mg L-1.
34780741	7	70	theme	environmental	1437:1449	arg1	concentration					1453:1465	environmental N concentration	1437:1465	environmental N concentration	1437:1465	Our results showed that, as the raw material and environmental signal, environmental N concentration regulates the elements (C and N) percentage of cyanobacterial cells and its EPSs excretion pattern, but not the monosaccharidic composition or the morphologies.
34780741	0	71	theme	EPS	82:84	arg1	excretion					86:94	cyanobacterial EPS excretion	67:94	cyanobacterial EPS excretion	67:94	Nitrogen concentration acting as an environmental signal regulates cyanobacterial EPS excretion.
34780741	2	72	from	influence	324:332	arg1	growth					456:461	Microcoleus vaginatusis growth	432:461	Microcoleus vaginatusis growth	432:461	In this study, the influence of initial N concentrations (10, 25 and 50 mg L-1 designated as N10, N25 and N50 respectively) on Microcoleus vaginatusis growth and the excretion of EPSs including RPS (released exopolysaccharides) and CPS (capsule exopolysaccharides) were evaluated at different growth periods.
34780741	3	73	located	observed	816:823	arg1	period					862:867	stationary period	851:867	stationary period	851:867	In logarithmic period, higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs was observed in the N50 group with the highest N concentration (about 40 mg L-1) in the medium, while no difference was observed among the three groups in stationary period when the N concentrations of medium were lower than 25 mg L-1.
34780741	3	73	located	observed	816:823	arg2	difference					801:810	no difference	798:810	no difference	798:810	In logarithmic period, higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs was observed in the N50 group with the highest N concentration (about 40 mg L-1) in the medium, while no difference was observed among the three groups in stationary period when the N concentrations of medium were lower than 25 mg L-1.
34780741	0	74	theme	cyanobacterial	67:80	arg1	excretion					86:94	cyanobacterial EPS excretion	67:94	cyanobacterial EPS excretion	67:94	Nitrogen concentration acting as an environmental signal regulates cyanobacterial EPS excretion.
34780741	1	75	theme	biological	135:144	arg1	BSCs					159:162	BSCs	159:162	BSCs	159:162	As an important carbon (C) storage in biological soil crusts (BSCs), exopolysaccharides (EPSs) are not only a part of the desert C cycle, but also the key materials for cyanobacteria to resist desert stress.
34780741	1	75	theme	biological	135:144	arg1	crusts					151:156	biological soil crusts	135:156	biological soil crusts (BSCs)	135:163	As an important carbon (C) storage in biological soil crusts (BSCs), exopolysaccharides (EPSs) are not only a part of the desert C cycle, but also the key materials for cyanobacteria to resist desert stress.
34780741	8	76	with	EPSs	1710:1713	arg1	proportion					1727:1736	higher proportion	1720:1736	higher proportion of CPS	1720:1743	These results also implied that, as the essential self-protecting materials, more EPSs with higher proportion of CPS would be excreted to response the low N environment.
34780741	3	77	theme	DW-CPS	683:688	arg1	/EPSs					690:694	(DW-CPS)/EPSs	682:694	(DW-CPS)/EPSs	682:694	In logarithmic period, higher ratio of biomass to EPSs indicated by (DW-CPS)/EPSs was observed in the N50 group with the highest N concentration (about 40 mg L-1) in the medium, while no difference was observed among the three groups in stationary period when the N concentrations of medium were lower than 25 mg L-1.
33973638	0	0	theme	coordinated	96:106	arg1	movement					108:115	coordinated movement	96:115	coordinated movement	96:115	Secreted C-type lectin regulation of neuromuscular junction synaptic vesicle dynamics modulates coordinated movement.
33973638	3	1	theme	neuromuscular	400:412	arg1	NMJ					424:426	NMJ	424:426	NMJ	424:426	Using the Drosophila neuromuscular junction (NMJ) as a model glutamatergic synapse, we identify a new Ca2+-binding (C-type) lectin, Lectin-galC1 (LGC1), which modulates presynaptic function and neurotransmission strength.
33973638	3	1	theme	neuromuscular	400:412	arg1	junction					414:421	the Drosophila neuromuscular junction	385:421	the Drosophila neuromuscular junction (NMJ)	385:427	Using the Drosophila neuromuscular junction (NMJ) as a model glutamatergic synapse, we identify a new Ca2+-binding (C-type) lectin, Lectin-galC1 (LGC1), which modulates presynaptic function and neurotransmission strength.
33973638	3	1	theme	neuromuscular	400:412	arg1	synapse					454:460	a model glutamatergic synapse	432:460	a model glutamatergic synapse	432:460	Using the Drosophila neuromuscular junction (NMJ) as a model glutamatergic synapse, we identify a new Ca2+-binding (C-type) lectin, Lectin-galC1 (LGC1), which modulates presynaptic function and neurotransmission strength.
33973638	1	2	theme	structured	176:185	arg1	glycans					187:193	structured glycans	176:193	structured glycans coordinating signaling between presynaptic and postsynaptic cells	176:259	The synaptic cleft manifests enriched glycosylation, with structured glycans coordinating signaling between presynaptic and postsynaptic cells.
33973638	4	3	theme	motoneuron	634:643	arg1	boutons					657:663	motoneuron presynaptic boutons	634:663	motoneuron presynaptic boutons	634:663	We find that LGC1 is enriched in motoneuron presynaptic boutons and secreted into the NMJ extracellular synaptomatrix.
33973638	6	4	theme	neurotransmission	890:906	arg1	strength					908:915	neurotransmission strength	890:915	neurotransmission strength	890:915	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	6	5	theme	active	941:946	arg1	zone					948:951	presynaptic active zone	929:951	presynaptic active zone (AZ)	929:956	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	6	5	theme	active	941:946	arg1	AZ					954:955	AZ	954:955	AZ	954:955	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	6	6	theme	vesicle	1083:1089	arg1	release					1096:1102	both spontaneous and stimulation-evoked synaptic vesicle (SV) release	1034:1102	both spontaneous and stimulation-evoked synaptic vesicle (SV) release	1034:1102	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	8	7	theme	presynaptic	1458:1468	arg1	zones					1477:1481	presynaptic active zones	1458:1481	presynaptic active zones	1458:1481	Although LGC1 removal increases the number of glutamatergic synapses, we find that LGC1-null mutants exhibit decreased SV density within presynaptic boutons, particularly SV pools at presynaptic active zones.
33973638	7	8	contain	have	1183:1186	arg2	faster					1188:1193	faster	1188:1193	faster	1188:1193	During high-frequency stimulation (HFS), mutants have faster synaptic depression and impaired recovery while replenishing depleted SV pools.
33973638	7	8	contain	have	1183:1186	arg1	mutants					1175:1181	mutants	1175:1181	mutants	1175:1181	During high-frequency stimulation (HFS), mutants have faster synaptic depression and impaired recovery while replenishing depleted SV pools.
33973638	6	9	theme	synaptic	1074:1081	arg1	release					1096:1102	both spontaneous and stimulation-evoked synaptic vesicle (SV) release	1034:1102	both spontaneous and stimulation-evoked synaptic vesicle (SV) release	1034:1102	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	3	10	theme	model	434:438	arg1	synapse					454:460	a model glutamatergic synapse	432:460	a model glutamatergic synapse	432:460	Using the Drosophila neuromuscular junction (NMJ) as a model glutamatergic synapse, we identify a new Ca2+-binding (C-type) lectin, Lectin-galC1 (LGC1), which modulates presynaptic function and neurotransmission strength.
33973638	3	10	theme	model	434:438	arg1	junction					414:421	the Drosophila neuromuscular junction	385:421	the Drosophila neuromuscular junction (NMJ)	385:427	Using the Drosophila neuromuscular junction (NMJ) as a model glutamatergic synapse, we identify a new Ca2+-binding (C-type) lectin, Lectin-galC1 (LGC1), which modulates presynaptic function and neurotransmission strength.
33973638	6	11	theme	glutamate	975:983	arg1	GluR					995:998	GluR	995:998	GluR	995:998	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	6	11	theme	glutamate	975:983	arg1	receptor					985:992	postsynaptic glutamate receptor	962:992	postsynaptic glutamate receptor (GluR)	962:999	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	2	12	theme	glycan-binding	355:368	arg1	lectins					370:376	secreted glycan-binding lectins	346:376	secreted glycan-binding lectins	346:376	Glycosylated signaling ligands orchestrating communication are tightly regulated by secreted glycan-binding lectins.
33973638	4	13	theme	presynaptic	645:655	arg1	boutons					657:663	motoneuron presynaptic boutons	634:663	motoneuron presynaptic boutons	634:663	We find that LGC1 is enriched in motoneuron presynaptic boutons and secreted into the NMJ extracellular synaptomatrix.
33973638	5	14	theme	specific	806:813	arg1	requirement					815:825	a specific requirement	804:825	a specific requirement for synaptic function, but not NMJ architecture	804:873	We show that LGC1 limits locomotor peristalsis and coordinated movement speed, with a specific requirement for synaptic function, but not NMJ architecture.
33973638	6	15	theme	postsynaptic	962:973	arg1	GluR					995:998	GluR	995:998	GluR	995:998	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	6	15	theme	postsynaptic	962:973	arg1	receptor					985:992	postsynaptic glutamate receptor	962:992	postsynaptic glutamate receptor (GluR)	962:999	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	4	16	theme	NMJ	687:689	arg1	synaptomatrix					705:717	the NMJ extracellular synaptomatrix	683:717	the NMJ extracellular synaptomatrix	683:717	We find that LGC1 is enriched in motoneuron presynaptic boutons and secreted into the NMJ extracellular synaptomatrix.
33973638	2	17	theme	secreted	346:353	arg1	lectins					370:376	secreted glycan-binding lectins	346:376	secreted glycan-binding lectins	346:376	Glycosylated signaling ligands orchestrating communication are tightly regulated by secreted glycan-binding lectins.
33973638	9	18	theme	coordinated	1539:1549	arg1	movement					1551:1558	coordinated movement	1539:1558	coordinated movement	1539:1558	Thus, LGC1 regulates NMJ neurotransmission to modulate coordinated movement.
33973638	6	19	theme	SV	1117:1118	arg1	rate					1128:1131	SV cycling rate	1117:1131	SV cycling rate	1117:1131	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	6	20	theme	stimulation-evoked	1055:1072	arg1	release					1096:1102	both spontaneous and stimulation-evoked synaptic vesicle (SV) release	1034:1102	both spontaneous and stimulation-evoked synaptic vesicle (SV) release	1034:1102	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	3	21	theme	new	477:479	arg1	Lectin-galC1					511:522	Lectin-galC1	511:522	Lectin-galC1 (LGC1)	511:529	Using the Drosophila neuromuscular junction (NMJ) as a model glutamatergic synapse, we identify a new Ca2+-binding (C-type) lectin, Lectin-galC1 (LGC1), which modulates presynaptic function and neurotransmission strength.
33973638	3	21	theme	new	477:479	arg1	lectin					503:508	a new Ca2+-binding (C-type) lectin	475:508	a new Ca2+-binding (C-type) lectin	475:508	Using the Drosophila neuromuscular junction (NMJ) as a model glutamatergic synapse, we identify a new Ca2+-binding (C-type) lectin, Lectin-galC1 (LGC1), which modulates presynaptic function and neurotransmission strength.
33973638	1	22	dep	manifests	137:145	arg1	enriched					147:154	enriched	147:154	enriched	147:154	The synaptic cleft manifests enriched glycosylation, with structured glycans coordinating signaling between presynaptic and postsynaptic cells.
33973638	7	23	theme	SV	1265:1266	arg1	pools					1268:1272	depleted SV pools	1256:1272	depleted SV pools	1256:1272	During high-frequency stimulation (HFS), mutants have faster synaptic depression and impaired recovery while replenishing depleted SV pools.
33973638	0	24	theme	C-type	9:14	arg1	lectin					16:21	Secreted C-type lectin	0:21	Secreted C-type lectin regulation of neuromuscular junction synaptic vesicle dynamics	0:84	Secreted C-type lectin regulation of neuromuscular junction synaptic vesicle dynamics modulates coordinated movement.
33973638	8	25	dep	pools	1449:1453	arg1	zones					1477:1481	presynaptic active zones	1458:1481	presynaptic active zones	1458:1481	Although LGC1 removal increases the number of glutamatergic synapses, we find that LGC1-null mutants exhibit decreased SV density within presynaptic boutons, particularly SV pools at presynaptic active zones.
33973638	8	26	theme	glutamatergic	1321:1333	arg1	synapses					1335:1342	glutamatergic synapses	1321:1342	glutamatergic synapses	1321:1342	Although LGC1 removal increases the number of glutamatergic synapses, we find that LGC1-null mutants exhibit decreased SV density within presynaptic boutons, particularly SV pools at presynaptic active zones.
33973638	5	27	theme	locomotor	745:753	arg1	peristalsis					755:765	locomotor peristalsis	745:765	locomotor peristalsis	745:765	We show that LGC1 limits locomotor peristalsis and coordinated movement speed, with a specific requirement for synaptic function, but not NMJ architecture.
33973638	8	28	theme	synapses	1335:1342	arg1	number					1311:1316	the number	1307:1316	the number of glutamatergic synapses	1307:1342	Although LGC1 removal increases the number of glutamatergic synapses, we find that LGC1-null mutants exhibit decreased SV density within presynaptic boutons, particularly SV pools at presynaptic active zones.
33973638	0	29	theme	Secreted	0:7	arg1	lectin					16:21	Secreted C-type lectin	0:21	Secreted C-type lectin regulation of neuromuscular junction synaptic vesicle dynamics	0:84	Secreted C-type lectin regulation of neuromuscular junction synaptic vesicle dynamics modulates coordinated movement.
33973638	9	30	theme	NMJ	1505:1507	arg1	neurotransmission					1509:1525	NMJ neurotransmission	1505:1525	NMJ neurotransmission	1505:1525	Thus, LGC1 regulates NMJ neurotransmission to modulate coordinated movement.
33973638	1	31	theme	presynaptic	226:236	arg1	cells					255:259	presynaptic and postsynaptic cells	226:259	presynaptic and postsynaptic cells	226:259	The synaptic cleft manifests enriched glycosylation, with structured glycans coordinating signaling between presynaptic and postsynaptic cells.
33973638	3	32	theme	glutamatergic	440:452	arg1	synapse					454:460	a model glutamatergic synapse	432:460	a model glutamatergic synapse	432:460	Using the Drosophila neuromuscular junction (NMJ) as a model glutamatergic synapse, we identify a new Ca2+-binding (C-type) lectin, Lectin-galC1 (LGC1), which modulates presynaptic function and neurotransmission strength.
33973638	3	32	theme	glutamatergic	440:452	arg1	junction					414:421	the Drosophila neuromuscular junction	385:421	the Drosophila neuromuscular junction (NMJ)	385:427	Using the Drosophila neuromuscular junction (NMJ) as a model glutamatergic synapse, we identify a new Ca2+-binding (C-type) lectin, Lectin-galC1 (LGC1), which modulates presynaptic function and neurotransmission strength.
33973638	6	33	theme	spontaneous	1039:1049	arg1	release					1096:1102	both spontaneous and stimulation-evoked synaptic vesicle (SV) release	1034:1102	both spontaneous and stimulation-evoked synaptic vesicle (SV) release	1034:1102	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	5	34	theme	coordinated	771:781	arg1	movement					783:790	coordinated movement speed	771:796	coordinated movement speed	771:796	We show that LGC1 limits locomotor peristalsis and coordinated movement speed, with a specific requirement for synaptic function, but not NMJ architecture.
33973638	5	35	theme	NMJ	858:860	arg1	architecture					862:873	NMJ architecture	858:873	NMJ architecture	858:873	We show that LGC1 limits locomotor peristalsis and coordinated movement speed, with a specific requirement for synaptic function, but not NMJ architecture.
33973638	4	36	theme	extracellular	691:703	arg1	synaptomatrix					705:717	the NMJ extracellular synaptomatrix	683:717	the NMJ extracellular synaptomatrix	683:717	We find that LGC1 is enriched in motoneuron presynaptic boutons and secreted into the NMJ extracellular synaptomatrix.
33973638	0	37	theme	lectin	16:21	arg1	regulation					23:32	Secreted C-type lectin regulation	0:32	Secreted C-type lectin regulation of neuromuscular junction synaptic vesicle dynamics	0:84	Secreted C-type lectin regulation of neuromuscular junction synaptic vesicle dynamics modulates coordinated movement.
33973638	8	38	theme	SV	1446:1447	arg1	pools					1449:1453	SV pools	1446:1453	SV pools	1446:1453	Although LGC1 removal increases the number of glutamatergic synapses, we find that LGC1-null mutants exhibit decreased SV density within presynaptic boutons, particularly SV pools at presynaptic active zones.
33973638	1	39	theme	synaptic	122:129	arg1	cleft					131:135	The synaptic cleft	118:135	The synaptic cleft	118:135	The synaptic cleft manifests enriched glycosylation, with structured glycans coordinating signaling between presynaptic and postsynaptic cells.
33973638	1	40	theme	postsynaptic	242:253	arg1	cells					255:259	presynaptic and postsynaptic cells	226:259	presynaptic and postsynaptic cells	226:259	The synaptic cleft manifests enriched glycosylation, with structured glycans coordinating signaling between presynaptic and postsynaptic cells.
33973638	0	41	theme	neuromuscular	37:49	arg1	junction					51:58	neuromuscular junction	37:58	neuromuscular junction synaptic vesicle dynamics	37:84	Secreted C-type lectin regulation of neuromuscular junction synaptic vesicle dynamics modulates coordinated movement.
33973638	6	42	dep	aligned	1001:1007	arg1	zone					948:951	presynaptic active zone	929:951	presynaptic active zone (AZ)	929:956	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	6	42	dep	aligned	1001:1007	arg1	GluR					995:998	GluR	995:998	GluR	995:998	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	6	42	dep	aligned	1001:1007	arg1	receptor					985:992	postsynaptic glutamate receptor	962:992	postsynaptic glutamate receptor (GluR)	962:999	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	6	42	dep	aligned	1001:1007	arg1	AZ					954:955	AZ	954:955	AZ	954:955	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	3	43	theme	C-type	495:500	arg1	Lectin-galC1					511:522	Lectin-galC1	511:522	Lectin-galC1 (LGC1)	511:529	Using the Drosophila neuromuscular junction (NMJ) as a model glutamatergic synapse, we identify a new Ca2+-binding (C-type) lectin, Lectin-galC1 (LGC1), which modulates presynaptic function and neurotransmission strength.
33973638	3	43	theme	C-type	495:500	arg1	lectin					503:508	a new Ca2+-binding (C-type) lectin	475:508	a new Ca2+-binding (C-type) lectin	475:508	Using the Drosophila neuromuscular junction (NMJ) as a model glutamatergic synapse, we identify a new Ca2+-binding (C-type) lectin, Lectin-galC1 (LGC1), which modulates presynaptic function and neurotransmission strength.
33973638	7	44	theme	synaptic	1195:1202	arg1	depression					1204:1213	synaptic depression	1195:1213	synaptic depression	1195:1213	During high-frequency stimulation (HFS), mutants have faster synaptic depression and impaired recovery while replenishing depleted SV pools.
33973638	3	45	theme	Ca2+-binding	481:492	arg1	Lectin-galC1					511:522	Lectin-galC1	511:522	Lectin-galC1 (LGC1)	511:529	Using the Drosophila neuromuscular junction (NMJ) as a model glutamatergic synapse, we identify a new Ca2+-binding (C-type) lectin, Lectin-galC1 (LGC1), which modulates presynaptic function and neurotransmission strength.
33973638	3	45	theme	Ca2+-binding	481:492	arg1	lectin					503:508	a new Ca2+-binding (C-type) lectin	475:508	a new Ca2+-binding (C-type) lectin	475:508	Using the Drosophila neuromuscular junction (NMJ) as a model glutamatergic synapse, we identify a new Ca2+-binding (C-type) lectin, Lectin-galC1 (LGC1), which modulates presynaptic function and neurotransmission strength.
33973638	7	46	theme	impaired	1219:1226	arg1	recovery					1228:1235	impaired recovery	1219:1235	impaired recovery	1219:1235	During high-frequency stimulation (HFS), mutants have faster synaptic depression and impaired recovery while replenishing depleted SV pools.
33973638	0	47	theme	synaptic	60:67	arg1	dynamics					77:84	neuromuscular junction synaptic vesicle dynamics	37:84	neuromuscular junction synaptic vesicle dynamics	37:84	Secreted C-type lectin regulation of neuromuscular junction synaptic vesicle dynamics modulates coordinated movement.
33973638	3	48	theme	presynaptic	548:558	arg1	function					560:567	presynaptic function	548:567	presynaptic function	548:567	Using the Drosophila neuromuscular junction (NMJ) as a model glutamatergic synapse, we identify a new Ca2+-binding (C-type) lectin, Lectin-galC1 (LGC1), which modulates presynaptic function and neurotransmission strength.
33973638	8	49	theme	LGC1	1284:1287	arg1	removal					1289:1295	LGC1 removal	1284:1295	LGC1 removal	1284:1295	Although LGC1 removal increases the number of glutamatergic synapses, we find that LGC1-null mutants exhibit decreased SV density within presynaptic boutons, particularly SV pools at presynaptic active zones.
33973638	5	50	theme	synaptic	831:838	arg1	function					840:847	synaptic function	831:847	synaptic function	831:847	We show that LGC1 limits locomotor peristalsis and coordinated movement speed, with a specific requirement for synaptic function, but not NMJ architecture.
33973638	2	51	theme	signaling	275:283	arg1	ligands					285:291	Glycosylated signaling ligands	262:291	Glycosylated signaling ligands orchestrating communication	262:319	Glycosylated signaling ligands orchestrating communication are tightly regulated by secreted glycan-binding lectins.
33973638	0	52	theme	junction	51:58	arg1	dynamics					77:84	neuromuscular junction synaptic vesicle dynamics	37:84	neuromuscular junction synaptic vesicle dynamics	37:84	Secreted C-type lectin regulation of neuromuscular junction synaptic vesicle dynamics modulates coordinated movement.
33973638	6	53	theme	synapse	1009:1015	arg1	number					1017:1022	presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number	929:1022	presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number	929:1022	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	3	54	theme	Drosophila	389:398	arg1	NMJ					424:426	NMJ	424:426	NMJ	424:426	Using the Drosophila neuromuscular junction (NMJ) as a model glutamatergic synapse, we identify a new Ca2+-binding (C-type) lectin, Lectin-galC1 (LGC1), which modulates presynaptic function and neurotransmission strength.
33973638	3	54	theme	Drosophila	389:398	arg1	junction					414:421	the Drosophila neuromuscular junction	385:421	the Drosophila neuromuscular junction (NMJ)	385:427	Using the Drosophila neuromuscular junction (NMJ) as a model glutamatergic synapse, we identify a new Ca2+-binding (C-type) lectin, Lectin-galC1 (LGC1), which modulates presynaptic function and neurotransmission strength.
33973638	3	54	theme	Drosophila	389:398	arg1	synapse					454:460	a model glutamatergic synapse	432:460	a model glutamatergic synapse	432:460	Using the Drosophila neuromuscular junction (NMJ) as a model glutamatergic synapse, we identify a new Ca2+-binding (C-type) lectin, Lectin-galC1 (LGC1), which modulates presynaptic function and neurotransmission strength.
33973638	6	55	theme	presynaptic	929:939	arg1	zone					948:951	presynaptic active zone	929:951	presynaptic active zone (AZ)	929:956	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	6	55	theme	presynaptic	929:939	arg1	AZ					954:955	AZ	954:955	AZ	954:955	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	2	56	theme	Glycosylated	262:273	arg1	ligands					285:291	Glycosylated signaling ligands	262:291	Glycosylated signaling ligands orchestrating communication	262:319	Glycosylated signaling ligands orchestrating communication are tightly regulated by secreted glycan-binding lectins.
33973638	8	57	theme	LGC1-null	1358:1366	arg1	mutants					1368:1374	LGC1-null mutants	1358:1374	LGC1-null mutants	1358:1374	Although LGC1 removal increases the number of glutamatergic synapses, we find that LGC1-null mutants exhibit decreased SV density within presynaptic boutons, particularly SV pools at presynaptic active zones.
33973638	6	58	theme	aligned	1001:1007	arg1	number					1017:1022	presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number	929:1022	presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number	929:1022	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	8	59	theme	decreased	1384:1392	arg1	density					1397:1403	decreased SV density	1384:1403	decreased SV density	1384:1403	Although LGC1 removal increases the number of glutamatergic synapses, we find that LGC1-null mutants exhibit decreased SV density within presynaptic boutons, particularly SV pools at presynaptic active zones.
33973638	8	60	theme	active	1470:1475	arg1	zones					1477:1481	presynaptic active zones	1458:1481	presynaptic active zones	1458:1481	Although LGC1 removal increases the number of glutamatergic synapses, we find that LGC1-null mutants exhibit decreased SV density within presynaptic boutons, particularly SV pools at presynaptic active zones.
33973638	8	61	theme	SV	1394:1395	arg1	density					1397:1403	decreased SV density	1384:1403	decreased SV density	1384:1403	Although LGC1 removal increases the number of glutamatergic synapses, we find that LGC1-null mutants exhibit decreased SV density within presynaptic boutons, particularly SV pools at presynaptic active zones.
33973638	8	62	theme	presynaptic	1412:1422	arg1	boutons					1424:1430	presynaptic boutons	1412:1430	presynaptic boutons	1412:1430	Although LGC1 removal increases the number of glutamatergic synapses, we find that LGC1-null mutants exhibit decreased SV density within presynaptic boutons, particularly SV pools at presynaptic active zones.
33973638	0	63	theme	dynamics	77:84	arg1	regulation					23:32	Secreted C-type lectin regulation	0:32	Secreted C-type lectin regulation of neuromuscular junction synaptic vesicle dynamics	0:84	Secreted C-type lectin regulation of neuromuscular junction synaptic vesicle dynamics modulates coordinated movement.
33973638	7	64	dep	stimulation	1156:1166	arg1	high-frequency					1141:1154	high-frequency	1141:1154	high-frequency	1141:1154	During high-frequency stimulation (HFS), mutants have faster synaptic depression and impaired recovery while replenishing depleted SV pools.
33973638	6	65	theme	cycling	1120:1126	arg1	rate					1128:1131	SV cycling rate	1117:1131	SV cycling rate	1117:1131	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33973638	0	66	theme	vesicle	69:75	arg1	dynamics					77:84	neuromuscular junction synaptic vesicle dynamics	37:84	neuromuscular junction synaptic vesicle dynamics	37:84	Secreted C-type lectin regulation of neuromuscular junction synaptic vesicle dynamics modulates coordinated movement.
33973638	7	67	dep	faster	1188:1193	arg1	depression					1204:1213	synaptic depression	1195:1213	synaptic depression	1195:1213	During high-frequency stimulation (HFS), mutants have faster synaptic depression and impaired recovery while replenishing depleted SV pools.
33973638	7	67	dep	faster	1188:1193	arg1	recovery					1228:1235	impaired recovery	1219:1235	impaired recovery	1219:1235	During high-frequency stimulation (HFS), mutants have faster synaptic depression and impaired recovery while replenishing depleted SV pools.
33973638	3	68	theme	neurotransmission	573:589	arg1	strength					591:598	neurotransmission strength	573:598	neurotransmission strength	573:598	Using the Drosophila neuromuscular junction (NMJ) as a model glutamatergic synapse, we identify a new Ca2+-binding (C-type) lectin, Lectin-galC1 (LGC1), which modulates presynaptic function and neurotransmission strength.
33973638	7	69	theme	depleted	1256:1263	arg1	pools					1268:1272	depleted SV pools	1256:1272	depleted SV pools	1256:1272	During high-frequency stimulation (HFS), mutants have faster synaptic depression and impaired recovery while replenishing depleted SV pools.
33973638	6	70	theme	SV	1092:1093	arg1	release					1096:1102	both spontaneous and stimulation-evoked synaptic vesicle (SV) release	1034:1102	both spontaneous and stimulation-evoked synaptic vesicle (SV) release	1034:1102	LGC1 controls neurotransmission strength by limiting presynaptic active zone (AZ) and postsynaptic glutamate receptor (GluR) aligned synapse number, reducing both spontaneous and stimulation-evoked synaptic vesicle (SV) release, and capping SV cycling rate.
33125976	3	0	theme	affinity	333:340	arg1	AAM					352:354	AAM	352:354	AAM	352:354	Herein, a novel anticoagulant affinity membrane (AAM) was developed to clear bilirubin from human blood in a pore-flow-through way.
33125976	3	0	theme	affinity	333:340	arg1	membrane					342:349	a novel anticoagulant affinity membrane	311:349	a novel anticoagulant affinity membrane (AAM)	311:355	Herein, a novel anticoagulant affinity membrane (AAM) was developed to clear bilirubin from human blood in a pore-flow-through way.
33125976	7	1	theme	effective	904:912	arg1	activity					928:935	effective anticoagulant activity	904:935	effective anticoagulant activity	904:935	Besides, the AAM exhibited effective anticoagulant activity in the measurement of clotting time, with suppressed thrombus formation, low hemolysis ratio, minimized platelet and leukocyte adhesion, and excellent biosafety.
33125976	1	2	theme	toxins	165:170	arg1	adsorption					151:160	specific adsorption	142:160	specific adsorption of toxins	142:170	Affinity membrane is widely employed to promote specific adsorption of toxins and reduce the blood purification therapeutic time.
33125976	4	3	theme	chemical	562:569	arg1	method					594:599	chemical vapor deposition (CVD) method	562:599	chemical vapor deposition (CVD) method	562:599	Firstly, a nylon net membrane with a regularly arranged pore as the matrix was coated with poly(pyrrole-3-carboxylic acid) via chemical vapor deposition (CVD) method.
33125976	7	4	theme	leukocyte	1054:1062	arg1	adhesion					1064:1071	minimized platelet and leukocyte adhesion	1031:1071	adhesion	1064:1071	Besides, the AAM exhibited effective anticoagulant activity in the measurement of clotting time, with suppressed thrombus formation, low hemolysis ratio, minimized platelet and leukocyte adhesion, and excellent biosafety.
33125976	6	5	theme	3-dimensional	781:793	arg1	architecture					805:816	the 3-dimensional molecular architecture	777:816	the 3-dimensional molecular architecture of PLA	777:823	Owing to the 3-dimensional molecular architecture of PLA, up to 86.1 % of bilirubin was efficiently cleared.
33125976	5	6	theme	bilirubin	662:670	arg1	ligand					652:657	a highly specific ligand	634:657	a highly specific ligand of bilirubin	634:670	Then, poly(L-arginine) (PLA) as a highly specific ligand of bilirubin, was immobilized onto the surface of the composited membrane after the modification of heparin.
33125976	6	7	theme	86.1	832:835	arg1	%					837:837	86.1 %	832:837	86.1 % of bilirubin	832:850	Owing to the 3-dimensional molecular architecture of PLA, up to 86.1 % of bilirubin was efficiently cleared.
33125976	6	7	theme	86.1	832:835	arg1	bilirubin					842:850	bilirubin	842:850	bilirubin	842:850	Owing to the 3-dimensional molecular architecture of PLA, up to 86.1 % of bilirubin was efficiently cleared.
33125976	7	8	theme	minimized	1031:1039	arg1	platelet					1041:1048	minimized platelet and leukocyte adhesion	1031:1071	platelet	1041:1048	Besides, the AAM exhibited effective anticoagulant activity in the measurement of clotting time, with suppressed thrombus formation, low hemolysis ratio, minimized platelet and leukocyte adhesion, and excellent biosafety.
33125976	4	9	theme	deposition	577:586	arg1	method					594:599	chemical vapor deposition (CVD) method	562:599	chemical vapor deposition (CVD) method	562:599	Firstly, a nylon net membrane with a regularly arranged pore as the matrix was coated with poly(pyrrole-3-carboxylic acid) via chemical vapor deposition (CVD) method.
33125976	8	10	dep	hemocompatibility	1185:1201	arg1	removal					1217:1223	removal	1217:1223	removal	1217:1223	Therefore, the AAM has enormous potential in blood purification therapy for enhancing hemocompatibility and bilirubin removal.
33125976	4	11	theme	vapor	571:575	arg1	method					594:599	chemical vapor deposition (CVD) method	562:599	chemical vapor deposition (CVD) method	562:599	Firstly, a nylon net membrane with a regularly arranged pore as the matrix was coated with poly(pyrrole-3-carboxylic acid) via chemical vapor deposition (CVD) method.
33125976	3	12	dep	novel	313:317	arg1	anticoagulant					319:331	anticoagulant	319:331	anticoagulant	319:331	Herein, a novel anticoagulant affinity membrane (AAM) was developed to clear bilirubin from human blood in a pore-flow-through way.
33125976	4	13	with	membrane	456:463	arg1	pore					491:494	a regularly arranged pore	470:494	a regularly arranged pore as the matrix	470:508	Firstly, a nylon net membrane with a regularly arranged pore as the matrix was coated with poly(pyrrole-3-carboxylic acid) via chemical vapor deposition (CVD) method.
33125976	8	14	theme	blood	1144:1148	arg1	therapy					1163:1169	blood purification therapy	1144:1169	blood purification therapy for enhancing hemocompatibility and bilirubin removal	1144:1223	Therefore, the AAM has enormous potential in blood purification therapy for enhancing hemocompatibility and bilirubin removal.
33125976	4	15	theme	arranged	482:489	arg1	pore					491:494	a regularly arranged pore	470:494	a regularly arranged pore as the matrix	470:508	Firstly, a nylon net membrane with a regularly arranged pore as the matrix was coated with poly(pyrrole-3-carboxylic acid) via chemical vapor deposition (CVD) method.
33125976	2	16	theme	low	280:282	arg1	hemocompatibility					284:300	low hemocompatibility	280:300	low hemocompatibility	280:300	However, it suffers from insufficient toxin binding and low hemocompatibility.
33125976	0	17	theme	novel	2:6	arg1	hemocompatibility					53:69	A novel anticoagulant affinity membrane for enhanced hemocompatibility	0:69	A novel anticoagulant affinity membrane for enhanced hemocompatibility	0:69	A novel anticoagulant affinity membrane for enhanced hemocompatibility and bilirubin removal.
33125976	6	18	theme	PLA	821:823	arg1	architecture					805:816	the 3-dimensional molecular architecture	777:816	the 3-dimensional molecular architecture of PLA	777:823	Owing to the 3-dimensional molecular architecture of PLA, up to 86.1 % of bilirubin was efficiently cleared.
33125976	7	19	theme	low	1010:1012	arg1	ratio					1024:1028	low hemolysis ratio	1010:1028	low hemolysis ratio	1010:1028	Besides, the AAM exhibited effective anticoagulant activity in the measurement of clotting time, with suppressed thrombus formation, low hemolysis ratio, minimized platelet and leukocyte adhesion, and excellent biosafety.
33125976	1	20	theme	Affinity	94:101	arg1	membrane					103:110	Affinity membrane	94:110	Affinity membrane	94:110	Affinity membrane is widely employed to promote specific adsorption of toxins and reduce the blood purification therapeutic time.
33125976	1	21	theme	blood	187:191	arg1	time					218:221	the blood purification therapeutic time	183:221	the blood purification therapeutic time	183:221	Affinity membrane is widely employed to promote specific adsorption of toxins and reduce the blood purification therapeutic time.
33125976	3	22	theme	human	395:399	arg1	blood					401:405	human blood	395:405	human blood	395:405	Herein, a novel anticoagulant affinity membrane (AAM) was developed to clear bilirubin from human blood in a pore-flow-through way.
33125976	4	23	theme	pyrrole-3-carboxylic	531:550	arg1	poly					526:529	poly	526:529	poly(pyrrole-3-carboxylic acid)	526:556	Firstly, a nylon net membrane with a regularly arranged pore as the matrix was coated with poly(pyrrole-3-carboxylic acid) via chemical vapor deposition (CVD) method.
33125976	4	23	theme	pyrrole-3-carboxylic	531:550	arg1	acid					552:555	pyrrole-3-carboxylic acid	531:555	pyrrole-3-carboxylic acid	531:555	Firstly, a nylon net membrane with a regularly arranged pore as the matrix was coated with poly(pyrrole-3-carboxylic acid) via chemical vapor deposition (CVD) method.
33125976	7	24	theme	hemolysis	1014:1022	arg1	ratio					1024:1028	low hemolysis ratio	1010:1028	low hemolysis ratio	1010:1028	Besides, the AAM exhibited effective anticoagulant activity in the measurement of clotting time, with suppressed thrombus formation, low hemolysis ratio, minimized platelet and leukocyte adhesion, and excellent biosafety.
33125976	1	25	theme	purification	193:204	arg1	time					218:221	the blood purification therapeutic time	183:221	the blood purification therapeutic time	183:221	Affinity membrane is widely employed to promote specific adsorption of toxins and reduce the blood purification therapeutic time.
33125976	0	26	theme	affinity	22:29	arg1	hemocompatibility					53:69	A novel anticoagulant affinity membrane for enhanced hemocompatibility	0:69	A novel anticoagulant affinity membrane for enhanced hemocompatibility	0:69	A novel anticoagulant affinity membrane for enhanced hemocompatibility and bilirubin removal.
33125976	1	27	theme	therapeutic	206:216	arg1	time					218:221	the blood purification therapeutic time	183:221	the blood purification therapeutic time	183:221	Affinity membrane is widely employed to promote specific adsorption of toxins and reduce the blood purification therapeutic time.
33125976	0	28	theme	anticoagulant	8:20	arg1	hemocompatibility					53:69	A novel anticoagulant affinity membrane for enhanced hemocompatibility	0:69	A novel anticoagulant affinity membrane for enhanced hemocompatibility	0:69	A novel anticoagulant affinity membrane for enhanced hemocompatibility and bilirubin removal.
33125976	5	29	theme	specific	643:650	arg1	ligand					652:657	a highly specific ligand	634:657	a highly specific ligand of bilirubin	634:670	Then, poly(L-arginine) (PLA) as a highly specific ligand of bilirubin, was immobilized onto the surface of the composited membrane after the modification of heparin.
33125976	4	30	theme	nylon	446:450	arg1	membrane					456:463	a nylon net membrane	444:463	a nylon net membrane with a regularly arranged pore as the matrix	444:508	Firstly, a nylon net membrane with a regularly arranged pore as the matrix was coated with poly(pyrrole-3-carboxylic acid) via chemical vapor deposition (CVD) method.
33125976	7	31	theme	excellent	1078:1086	arg1	biosafety					1088:1096	excellent biosafety	1078:1096	excellent biosafety	1078:1096	Besides, the AAM exhibited effective anticoagulant activity in the measurement of clotting time, with suppressed thrombus formation, low hemolysis ratio, minimized platelet and leukocyte adhesion, and excellent biosafety.
33125976	0	32	theme	for	40:42	arg1	hemocompatibility					53:69	A novel anticoagulant affinity membrane for enhanced hemocompatibility	0:69	A novel anticoagulant affinity membrane for enhanced hemocompatibility	0:69	A novel anticoagulant affinity membrane for enhanced hemocompatibility and bilirubin removal.
33125976	7	33	theme	suppressed	979:988	arg1	formation					999:1007	suppressed thrombus formation	979:1007	suppressed thrombus formation	979:1007	Besides, the AAM exhibited effective anticoagulant activity in the measurement of clotting time, with suppressed thrombus formation, low hemolysis ratio, minimized platelet and leukocyte adhesion, and excellent biosafety.
33125976	0	34	theme	membrane	31:38	arg1	hemocompatibility					53:69	A novel anticoagulant affinity membrane for enhanced hemocompatibility	0:69	A novel anticoagulant affinity membrane for enhanced hemocompatibility	0:69	A novel anticoagulant affinity membrane for enhanced hemocompatibility and bilirubin removal.
33125976	3	35	theme	pore-flow-through	412:428	arg1	way					430:432	a pore-flow-through way	410:432	a pore-flow-through way	410:432	Herein, a novel anticoagulant affinity membrane (AAM) was developed to clear bilirubin from human blood in a pore-flow-through way.
33125976	6	36	theme	molecular	795:803	arg1	architecture					805:816	the 3-dimensional molecular architecture	777:816	the 3-dimensional molecular architecture of PLA	777:823	Owing to the 3-dimensional molecular architecture of PLA, up to 86.1 % of bilirubin was efficiently cleared.
33125976	8	37	theme	enormous	1122:1129	arg1	potential					1131:1139	enormous potential	1122:1139	enormous potential	1122:1139	Therefore, the AAM has enormous potential in blood purification therapy for enhancing hemocompatibility and bilirubin removal.
33125976	5	38	theme	membrane	724:731	arg1	surface					698:704	the surface	694:704	the surface of the composited membrane	694:731	Then, poly(L-arginine) (PLA) as a highly specific ligand of bilirubin, was immobilized onto the surface of the composited membrane after the modification of heparin.
33125976	3	39	theme	novel	313:317	arg1	AAM					352:354	AAM	352:354	AAM	352:354	Herein, a novel anticoagulant affinity membrane (AAM) was developed to clear bilirubin from human blood in a pore-flow-through way.
33125976	3	39	theme	novel	313:317	arg1	membrane					342:349	a novel anticoagulant affinity membrane	311:349	a novel anticoagulant affinity membrane (AAM)	311:355	Herein, a novel anticoagulant affinity membrane (AAM) was developed to clear bilirubin from human blood in a pore-flow-through way.
33125976	4	40	theme	net	452:454	arg1	membrane					456:463	a nylon net membrane	444:463	a nylon net membrane with a regularly arranged pore as the matrix	444:508	Firstly, a nylon net membrane with a regularly arranged pore as the matrix was coated with poly(pyrrole-3-carboxylic acid) via chemical vapor deposition (CVD) method.
33125976	7	41	theme	thrombus	990:997	arg1	formation					999:1007	suppressed thrombus formation	979:1007	suppressed thrombus formation	979:1007	Besides, the AAM exhibited effective anticoagulant activity in the measurement of clotting time, with suppressed thrombus formation, low hemolysis ratio, minimized platelet and leukocyte adhesion, and excellent biosafety.
33125976	0	42	theme	enhanced	44:51	arg1	hemocompatibility					53:69	A novel anticoagulant affinity membrane for enhanced hemocompatibility	0:69	A novel anticoagulant affinity membrane for enhanced hemocompatibility	0:69	A novel anticoagulant affinity membrane for enhanced hemocompatibility and bilirubin removal.
33125976	6	43	theme	bilirubin	842:850	arg1	%					837:837	86.1 %	832:837	86.1 % of bilirubin	832:850	Owing to the 3-dimensional molecular architecture of PLA, up to 86.1 % of bilirubin was efficiently cleared.
33125976	6	43	theme	bilirubin	842:850	arg1	bilirubin					842:850	bilirubin	842:850	bilirubin	842:850	Owing to the 3-dimensional molecular architecture of PLA, up to 86.1 % of bilirubin was efficiently cleared.
33125976	7	44	theme	clotting	959:966	arg1	time					968:971	clotting time	959:971	clotting time	959:971	Besides, the AAM exhibited effective anticoagulant activity in the measurement of clotting time, with suppressed thrombus formation, low hemolysis ratio, minimized platelet and leukocyte adhesion, and excellent biosafety.
33125976	8	45	contain	has	1118:1120	arg1	AAM					1114:1116	the AAM	1110:1116	the AAM	1110:1116	Therefore, the AAM has enormous potential in blood purification therapy for enhancing hemocompatibility and bilirubin removal.
33125976	8	45	contain	has	1118:1120	arg2	potential					1131:1139	enormous potential	1122:1139	enormous potential	1122:1139	Therefore, the AAM has enormous potential in blood purification therapy for enhancing hemocompatibility and bilirubin removal.
33125976	5	46	theme	composited	713:722	arg1	membrane					724:731	the composited membrane	709:731	the composited membrane	709:731	Then, poly(L-arginine) (PLA) as a highly specific ligand of bilirubin, was immobilized onto the surface of the composited membrane after the modification of heparin.
33125976	4	47	theme	CVD	589:591	arg1	method					594:599	chemical vapor deposition (CVD) method	562:599	chemical vapor deposition (CVD) method	562:599	Firstly, a nylon net membrane with a regularly arranged pore as the matrix was coated with poly(pyrrole-3-carboxylic acid) via chemical vapor deposition (CVD) method.
33125976	7	48	theme	time	968:971	arg1	measurement					944:954	the measurement	940:954	the measurement of clotting time	940:971	Besides, the AAM exhibited effective anticoagulant activity in the measurement of clotting time, with suppressed thrombus formation, low hemolysis ratio, minimized platelet and leukocyte adhesion, and excellent biosafety.
33125976	5	49	theme	heparin	759:765	arg1	modification					743:754	the modification	739:754	the modification of heparin	739:765	Then, poly(L-arginine) (PLA) as a highly specific ligand of bilirubin, was immobilized onto the surface of the composited membrane after the modification of heparin.
33125976	7	50	theme	anticoagulant	914:926	arg1	activity					928:935	effective anticoagulant activity	904:935	effective anticoagulant activity	904:935	Besides, the AAM exhibited effective anticoagulant activity in the measurement of clotting time, with suppressed thrombus formation, low hemolysis ratio, minimized platelet and leukocyte adhesion, and excellent biosafety.
33125976	1	51	theme	specific	142:149	arg1	adsorption					151:160	specific adsorption	142:160	specific adsorption of toxins	142:170	Affinity membrane is widely employed to promote specific adsorption of toxins and reduce the blood purification therapeutic time.
33125976	0	52	theme	bilirubin	75:83	arg1	removal					85:91	bilirubin removal	75:91	bilirubin removal	75:91	A novel anticoagulant affinity membrane for enhanced hemocompatibility and bilirubin removal.
33125976	2	53	theme	toxin	262:266	arg1	binding					268:274	insufficient toxin binding	249:274	insufficient toxin binding	249:274	However, it suffers from insufficient toxin binding and low hemocompatibility.
33125976	8	54	theme	purification	1150:1161	arg1	therapy					1163:1169	blood purification therapy	1144:1169	blood purification therapy for enhancing hemocompatibility and bilirubin removal	1144:1223	Therefore, the AAM has enormous potential in blood purification therapy for enhancing hemocompatibility and bilirubin removal.
33125976	3	55	from	blood	401:405	arg1	bilirubin					380:388	bilirubin	380:388	bilirubin from human blood	380:405	Herein, a novel anticoagulant affinity membrane (AAM) was developed to clear bilirubin from human blood in a pore-flow-through way.
33125976	2	56	theme	insufficient	249:260	arg1	binding					268:274	insufficient toxin binding	249:274	insufficient toxin binding	249:274	However, it suffers from insufficient toxin binding and low hemocompatibility.
33459814	1	0	from	effects	156:162	arg1	structure					207:215	structure	207:215	structure	207:215	In this study, the effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides (EPSs) produced by Lactobacillus delbrueckii subsp.
33459814	1	0	from	effects	156:162	arg1	composition					194:204	composition	194:204	composition	194:204	In this study, the effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides (EPSs) produced by Lactobacillus delbrueckii subsp.
33459814	1	0	from	effects	156:162	arg1	bioactivity					221:231	bioactivity	221:231	bioactivity	221:231	In this study, the effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides (EPSs) produced by Lactobacillus delbrueckii subsp.
33459814	5	1	from	Alterations	738:748	arg1	groups					768:773	the functional groups	753:773	the functional groups	753:773	Alterations in the functional groups, thermal and morphological characteristics of EPSs following the GSM 1800 band radiation application were confirmed by FTIR, TGA and SEM analysis, respectively.
33459814	4	2	theme	molecular	647:655	arg1	weights					657:663	molecular weights	647:663	molecular weights of the EPSs	647:675	An alteration in the chemical composition of the EPSs was observed and EPS production levels and molecular weights of the EPSs increased following the GSM 1800 band radiation application.
33459814	0	3	theme	exopolysaccharides	80:97	arg1	bioactivity					65:75	bioactivity	65:75	bioactivity	65:75	Effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides produced by yoghurt starter cultures.
33459814	0	3	theme	exopolysaccharides	80:97	arg1	structure					51:59	structure	51:59	structure	51:59	Effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides produced by yoghurt starter cultures.
33459814	0	3	theme	exopolysaccharides	80:97	arg1	composition					38:48	composition	38:48	composition	38:48	Effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides produced by yoghurt starter cultures.
33459814	0	4	from	Effects	0:6	arg1	bioactivity					65:75	bioactivity	65:75	bioactivity	65:75	Effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides produced by yoghurt starter cultures.
33459814	0	4	from	Effects	0:6	arg1	structure					51:59	structure	51:59	structure	51:59	Effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides produced by yoghurt starter cultures.
33459814	0	4	from	Effects	0:6	arg1	composition					38:48	composition	38:48	composition	38:48	Effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides produced by yoghurt starter cultures.
33459814	3	5	theme	stressed	513:520	arg1	R					530:530	R	530:530	R	530:530	For this, GSM 1800 band radiation was applied to both cultures and characteristics of EPSs extracted from the control groups (K) and the radiation stressed groups (R) were determined.
33459814	3	5	theme	stressed	513:520	arg1	groups					522:527	the radiation stressed groups	499:527	the radiation stressed groups (R)	499:531	For this, GSM 1800 band radiation was applied to both cultures and characteristics of EPSs extracted from the control groups (K) and the radiation stressed groups (R) were determined.
33459814	1	6	theme	exopolysaccharides	236:253	arg1	structure					207:215	structure	207:215	structure	207:215	In this study, the effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides (EPSs) produced by Lactobacillus delbrueckii subsp.
33459814	1	6	theme	exopolysaccharides	236:253	arg1	composition					194:204	composition	194:204	composition	194:204	In this study, the effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides (EPSs) produced by Lactobacillus delbrueckii subsp.
33459814	1	6	theme	exopolysaccharides	236:253	arg1	bioactivity					221:231	bioactivity	221:231	bioactivity	221:231	In this study, the effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides (EPSs) produced by Lactobacillus delbrueckii subsp.
33459814	4	7	theme	band	710:713	arg1	application					725:735	the GSM 1800 band radiation application	697:735	the GSM 1800 band radiation application	697:735	An alteration in the chemical composition of the EPSs was observed and EPS production levels and molecular weights of the EPSs increased following the GSM 1800 band radiation application.
33459814	7	8	from	characteristics	1138:1152	arg1	cultures					1182:1189	yogurt starter cultures	1167:1189	yogurt starter cultures	1167:1189	These results suggested the effects of the GSM radiation on final characteristics of EPSs from yogurt starter cultures.
33459814	6	9	from	alterations	951:961	arg1	activity					1000:1007	the antioxidant and antibacterial activity	966:1007	activity	1000:1007	Importantly no alterations in the antioxidant and antibacterial activity of the EPSs were observed following the radiation application.
33459814	6	10	theme	EPSs	1016:1019	arg1	activity					1000:1007	the antioxidant and antibacterial activity	966:1007	activity	1000:1007	Importantly no alterations in the antioxidant and antibacterial activity of the EPSs were observed following the radiation application.
33459814	5	11	theme	GSM	840:842	arg1	application					864:874	the GSM 1800 band radiation application	836:874	the GSM 1800 band radiation application	836:874	Alterations in the functional groups, thermal and morphological characteristics of EPSs following the GSM 1800 band radiation application were confirmed by FTIR, TGA and SEM analysis, respectively.
33459814	6	12	theme	radiation	1049:1057	arg1	application					1059:1069	the radiation application	1045:1069	the radiation application	1045:1069	Importantly no alterations in the antioxidant and antibacterial activity of the EPSs were observed following the radiation application.
33459814	7	13	theme	yogurt	1167:1172	arg1	cultures					1182:1189	yogurt starter cultures	1167:1189	yogurt starter cultures	1167:1189	These results suggested the effects of the GSM radiation on final characteristics of EPSs from yogurt starter cultures.
33459814	7	14	theme	radiation	1119:1127	arg1	effects					1100:1106	the effects	1096:1106	the effects of the GSM radiation on final characteristics of EPSs from yogurt starter cultures	1096:1189	These results suggested the effects of the GSM radiation on final characteristics of EPSs from yogurt starter cultures.
33459814	7	15	theme	starter	1174:1180	arg1	cultures					1182:1189	yogurt starter cultures	1167:1189	yogurt starter cultures	1167:1189	These results suggested the effects of the GSM radiation on final characteristics of EPSs from yogurt starter cultures.
33459814	3	16	theme	radiation	503:511	arg1	R					530:530	R	530:530	R	530:530	For this, GSM 1800 band radiation was applied to both cultures and characteristics of EPSs extracted from the control groups (K) and the radiation stressed groups (R) were determined.
33459814	3	16	theme	radiation	503:511	arg1	groups					522:527	the radiation stressed groups	499:527	the radiation stressed groups (R)	499:531	For this, GSM 1800 band radiation was applied to both cultures and characteristics of EPSs extracted from the control groups (K) and the radiation stressed groups (R) were determined.
33459814	3	17	theme	GSM	376:378	arg1	radiation					390:398	GSM 1800 band radiation	376:398	GSM 1800 band radiation	376:398	For this, GSM 1800 band radiation was applied to both cultures and characteristics of EPSs extracted from the control groups (K) and the radiation stressed groups (R) were determined.
33459814	5	18	theme	TGA	900:902	arg1	analysis					912:919	FTIR, TGA and SEM analysis	894:919	FTIR, TGA and SEM analysis	894:919	Alterations in the functional groups, thermal and morphological characteristics of EPSs following the GSM 1800 band radiation application were confirmed by FTIR, TGA and SEM analysis, respectively.
33459814	4	19	theme	production	625:634	arg1	levels					636:641	EPS production levels	621:641	EPS production levels	621:641	An alteration in the chemical composition of the EPSs was observed and EPS production levels and molecular weights of the EPSs increased following the GSM 1800 band radiation application.
33459814	3	20	theme	1800	380:383	arg1	radiation					390:398	GSM 1800 band radiation	376:398	GSM 1800 band radiation	376:398	For this, GSM 1800 band radiation was applied to both cultures and characteristics of EPSs extracted from the control groups (K) and the radiation stressed groups (R) were determined.
33459814	4	21	from	alteration	553:562	arg1	composition					580:590	the chemical composition	567:590	the chemical composition of the EPSs	567:602	An alteration in the chemical composition of the EPSs was observed and EPS production levels and molecular weights of the EPSs increased following the GSM 1800 band radiation application.
33459814	1	22	theme	GSM	167:169	arg1	radiation					181:189	GSM 1800 band radiation	167:189	GSM 1800 band radiation	167:189	In this study, the effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides (EPSs) produced by Lactobacillus delbrueckii subsp.
33459814	0	23	theme	1800	15:18	arg1	radiation					25:33	GSM 1800 band radiation	11:33	GSM 1800 band radiation	11:33	Effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides produced by yoghurt starter cultures.
33459814	0	24	theme	starter	119:125	arg1	cultures					127:134	yoghurt starter cultures	111:134	yoghurt starter cultures	111:134	Effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides produced by yoghurt starter cultures.
33459814	4	25	theme	1800	705:708	arg1	application					725:735	the GSM 1800 band radiation application	697:735	the GSM 1800 band radiation application	697:735	An alteration in the chemical composition of the EPSs was observed and EPS production levels and molecular weights of the EPSs increased following the GSM 1800 band radiation application.
33459814	3	26	theme	EPSs	452:455	arg1	characteristics					433:447	characteristics	433:447	characteristics of EPSs extracted from the control groups (K) and the radiation stressed groups (R)	433:531	For this, GSM 1800 band radiation was applied to both cultures and characteristics of EPSs extracted from the control groups (K) and the radiation stressed groups (R) were determined.
33459814	0	27	theme	GSM	11:13	arg1	radiation					25:33	GSM 1800 band radiation	11:33	GSM 1800 band radiation	11:33	Effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides produced by yoghurt starter cultures.
33459814	0	28	theme	yoghurt	111:117	arg1	cultures					127:134	yoghurt starter cultures	111:134	yoghurt starter cultures	111:134	Effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides produced by yoghurt starter cultures.
33459814	4	29	theme	GSM	701:703	arg1	application					725:735	the GSM 1800 band radiation application	697:735	the GSM 1800 band radiation application	697:735	An alteration in the chemical composition of the EPSs was observed and EPS production levels and molecular weights of the EPSs increased following the GSM 1800 band radiation application.
33459814	1	30	theme	band	176:179	arg1	radiation					181:189	GSM 1800 band radiation	167:189	GSM 1800 band radiation	167:189	In this study, the effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides (EPSs) produced by Lactobacillus delbrueckii subsp.
33459814	0	31	theme	radiation	25:33	arg1	Effects					0:6	Effects	0:6	Effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides	0:97	Effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides produced by yoghurt starter cultures.
33459814	7	32	theme	EPSs	1157:1160	arg1	characteristics					1138:1152	final characteristics	1132:1152	final characteristics of EPSs from yogurt starter cultures	1132:1189	These results suggested the effects of the GSM radiation on final characteristics of EPSs from yogurt starter cultures.
33459814	4	33	theme	EPS	621:623	arg1	production					625:634	EPS production	621:634	EPS production levels	621:641	An alteration in the chemical composition of the EPSs was observed and EPS production levels and molecular weights of the EPSs increased following the GSM 1800 band radiation application.
33459814	1	34	theme	radiation	181:189	arg1	effects					156:162	the effects	152:162	the effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides (EPSs)	152:260	In this study, the effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides (EPSs) produced by Lactobacillus delbrueckii subsp.
33459814	0	35	theme	band	20:23	arg1	radiation					25:33	GSM 1800 band radiation	11:33	GSM 1800 band radiation	11:33	Effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides produced by yoghurt starter cultures.
33459814	1	36	theme	Lactobacillus	274:286	arg1	subsp					300:304	Lactobacillus delbrueckii subsp	274:304	Lactobacillus delbrueckii subsp	274:304	In this study, the effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides (EPSs) produced by Lactobacillus delbrueckii subsp.
33459814	7	37	theme	final	1132:1136	arg1	characteristics					1138:1152	final characteristics	1132:1152	final characteristics of EPSs from yogurt starter cultures	1132:1189	These results suggested the effects of the GSM radiation on final characteristics of EPSs from yogurt starter cultures.
33459814	4	38	theme	EPSs	599:602	arg1	composition					580:590	the chemical composition	567:590	the chemical composition of the EPSs	567:602	An alteration in the chemical composition of the EPSs was observed and EPS production levels and molecular weights of the EPSs increased following the GSM 1800 band radiation application.
33459814	3	39	theme	band	385:388	arg1	radiation					390:398	GSM 1800 band radiation	376:398	GSM 1800 band radiation	376:398	For this, GSM 1800 band radiation was applied to both cultures and characteristics of EPSs extracted from the control groups (K) and the radiation stressed groups (R) were determined.
33459814	1	40	theme	delbrueckii	288:298	arg1	subsp					300:304	Lactobacillus delbrueckii subsp	274:304	Lactobacillus delbrueckii subsp	274:304	In this study, the effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides (EPSs) produced by Lactobacillus delbrueckii subsp.
33459814	7	41	from	cultures	1182:1189	arg1	characteristics					1138:1152	final characteristics	1132:1152	final characteristics of EPSs from yogurt starter cultures	1132:1189	These results suggested the effects of the GSM radiation on final characteristics of EPSs from yogurt starter cultures.
33459814	7	41	from	cultures	1182:1189	arg1	EPSs					1157:1160	EPSs	1157:1160	EPSs from yogurt starter cultures	1157:1189	These results suggested the effects of the GSM radiation on final characteristics of EPSs from yogurt starter cultures.
33459814	3	42	theme	control	476:482	arg1	K					492:492	K	492:492	K	492:492	For this, GSM 1800 band radiation was applied to both cultures and characteristics of EPSs extracted from the control groups (K) and the radiation stressed groups (R) were determined.
33459814	3	42	theme	control	476:482	arg1	groups					484:489	the control groups	472:489	the control groups (K)	472:493	For this, GSM 1800 band radiation was applied to both cultures and characteristics of EPSs extracted from the control groups (K) and the radiation stressed groups (R) were determined.
33459814	5	43	theme	functional	757:766	arg1	groups					768:773	the functional groups	753:773	the functional groups	753:773	Alterations in the functional groups, thermal and morphological characteristics of EPSs following the GSM 1800 band radiation application were confirmed by FTIR, TGA and SEM analysis, respectively.
33459814	7	44	from	effects	1100:1106	arg1	characteristics					1138:1152	final characteristics	1132:1152	final characteristics of EPSs from yogurt starter cultures	1132:1189	These results suggested the effects of the GSM radiation on final characteristics of EPSs from yogurt starter cultures.
33459814	7	45	theme	GSM	1115:1117	arg1	radiation					1119:1127	the GSM radiation	1111:1127	the GSM radiation	1111:1127	These results suggested the effects of the GSM radiation on final characteristics of EPSs from yogurt starter cultures.
33459814	5	46	from	characteristics	802:816	arg1	groups					768:773	the functional groups	753:773	the functional groups	753:773	Alterations in the functional groups, thermal and morphological characteristics of EPSs following the GSM 1800 band radiation application were confirmed by FTIR, TGA and SEM analysis, respectively.
33459814	5	47	theme	FTIR	894:897	arg1	analysis					912:919	FTIR, TGA and SEM analysis	894:919	FTIR, TGA and SEM analysis	894:919	Alterations in the functional groups, thermal and morphological characteristics of EPSs following the GSM 1800 band radiation application were confirmed by FTIR, TGA and SEM analysis, respectively.
33459814	5	48	theme	SEM	908:910	arg1	analysis					912:919	FTIR, TGA and SEM analysis	894:919	FTIR, TGA and SEM analysis	894:919	Alterations in the functional groups, thermal and morphological characteristics of EPSs following the GSM 1800 band radiation application were confirmed by FTIR, TGA and SEM analysis, respectively.
33459814	4	49	theme	radiation	715:723	arg1	application					725:735	the GSM 1800 band radiation application	697:735	the GSM 1800 band radiation application	697:735	An alteration in the chemical composition of the EPSs was observed and EPS production levels and molecular weights of the EPSs increased following the GSM 1800 band radiation application.
33459814	4	50	theme	EPSs	672:675	arg1	levels					636:641	EPS production levels	621:641	EPS production levels	621:641	An alteration in the chemical composition of the EPSs was observed and EPS production levels and molecular weights of the EPSs increased following the GSM 1800 band radiation application.
33459814	4	50	theme	EPSs	672:675	arg1	weights					657:663	molecular weights	647:663	molecular weights of the EPSs	647:675	An alteration in the chemical composition of the EPSs was observed and EPS production levels and molecular weights of the EPSs increased following the GSM 1800 band radiation application.
33459814	6	51	theme	antibacterial	986:998	arg1	activity					1000:1007	the antioxidant and antibacterial activity	966:1007	activity	1000:1007	Importantly no alterations in the antioxidant and antibacterial activity of the EPSs were observed following the radiation application.
33459814	5	52	theme	EPSs	821:824	arg1	Alterations					738:748	Alterations	738:748	Alterations in the functional groups	738:773	Alterations in the functional groups, thermal and morphological characteristics of EPSs following the GSM 1800 band radiation application were confirmed by FTIR, TGA and SEM analysis, respectively.
33459814	5	52	theme	EPSs	821:824	arg1	characteristics					802:816	morphological characteristics	788:816	morphological characteristics of EPSs following the GSM 1800 band radiation application	788:874	Alterations in the functional groups, thermal and morphological characteristics of EPSs following the GSM 1800 band radiation application were confirmed by FTIR, TGA and SEM analysis, respectively.
33459814	5	53	theme	morphological	788:800	arg1	characteristics					802:816	morphological characteristics	788:816	morphological characteristics of EPSs following the GSM 1800 band radiation application	788:874	Alterations in the functional groups, thermal and morphological characteristics of EPSs following the GSM 1800 band radiation application were confirmed by FTIR, TGA and SEM analysis, respectively.
33459814	5	54	theme	band	849:852	arg1	application					864:874	the GSM 1800 band radiation application	836:874	the GSM 1800 band radiation application	836:874	Alterations in the functional groups, thermal and morphological characteristics of EPSs following the GSM 1800 band radiation application were confirmed by FTIR, TGA and SEM analysis, respectively.
33459814	6	55	theme	antioxidant	970:980	arg1	activity					1000:1007	the antioxidant and antibacterial activity	966:1007	activity	1000:1007	Importantly no alterations in the antioxidant and antibacterial activity of the EPSs were observed following the radiation application.
33459814	5	56	theme	radiation	854:862	arg1	application					864:874	the GSM 1800 band radiation application	836:874	the GSM 1800 band radiation application	836:874	Alterations in the functional groups, thermal and morphological characteristics of EPSs following the GSM 1800 band radiation application were confirmed by FTIR, TGA and SEM analysis, respectively.
33459814	4	57	theme	chemical	571:578	arg1	composition					580:590	the chemical composition	567:590	the chemical composition of the EPSs	567:602	An alteration in the chemical composition of the EPSs was observed and EPS production levels and molecular weights of the EPSs increased following the GSM 1800 band radiation application.
34770809	0	0	theme	Traditional	99:109	arg1	Biscuit					128:134	Traditional Brazilian Starch Biscuit	99:134	Traditional Brazilian Starch Biscuit	99:134	Microencapsulated and Lyophilized Propolis Co-Product Extract as Antioxidant Synthetic Replacer on Traditional Brazilian Starch Biscuit.
34770809	3	1	theme	lyophilized	688:698	arg1	co-product					709:718	lyophilized propolis co-product	688:718	lyophilized propolis co-product (LFCP)	688:725	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	1	theme	lyophilized	688:698	arg1	days					565:568	45 days	562:568	45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP)	562:725	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	1	theme	lyophilized	688:698	arg1	LFCP					721:724	LFCP	721:724	LFCP	721:724	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	2	theme	starch	505:510	arg1	biscuit					512:518	starch biscuit	505:518	starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP)	505:725	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	3	theme	activity	809:816	arg1	analysis					752:759	analysis	752:759	analysis of their total phenolic content and antioxidant activity (AA)	752:821	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	7	4	located	found	1213:1217	arg2	acids					1202:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids	1137:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids	1137:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids were found in biscuits at constant concentrations throughout the storage period.
34770809	7	4	located	found	1213:1217	arg1	biscuits					1222:1229	biscuits	1222:1229	biscuits at constant concentrations	1222:1256	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids were found in biscuits at constant concentrations throughout the storage period.
34770809	4	5	theme	antioxidant	833:843	arg1	activity					845:852	Relevant antioxidant activity	824:852	Relevant antioxidant activity	824:852	Relevant antioxidant activity was observed using the methods of analysis employed.
34770809	8	6	theme	extracts	1396:1403	arg1	addition					1359:1366	the addition	1355:1366	the addition of both propolis co-product extracts	1355:1403	In addition, there was a reduction in malondialdehyde values with the addition of both propolis co-product extracts.
34770809	9	7	theme	lipid	1503:1507	arg1	oxidation					1509:1517	lipid oxidation	1503:1517	lipid oxidation	1503:1517	Therefore, the propolis co-product extracts could be utilized as a natural antioxidant to reduce lipid oxidation in fatty starch biscuit.
34770809	0	8	theme	Starch	121:126	arg1	Biscuit					128:134	Traditional Brazilian Starch Biscuit	99:134	Traditional Brazilian Starch Biscuit	99:134	Microencapsulated and Lyophilized Propolis Co-Product Extract as Antioxidant Synthetic Replacer on Traditional Brazilian Starch Biscuit.
34770809	7	9	theme	storage	1273:1279	arg1	period					1281:1286	the storage period	1269:1286	the storage period	1269:1286	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids were found in biscuits at constant concentrations throughout the storage period.
34770809	7	10	theme	linoleic	1176:1183	arg1	acids					1202:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids	1137:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids	1137:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids were found in biscuits at constant concentrations throughout the storage period.
34770809	2	11	from	oxidation	333:341	arg1	goods					357:361	baked goods	351:361	baked goods	351:361	However, among the challenges for using the propolis co-product as an inhibitor of lipid oxidation (LO) in baked goods is maintaining its bioactive compounds.
34770809	0	12	theme	Brazilian	111:119	arg1	Biscuit					128:134	Traditional Brazilian Starch Biscuit	99:134	Traditional Brazilian Starch Biscuit	99:134	Microencapsulated and Lyophilized Propolis Co-Product Extract as Antioxidant Synthetic Replacer on Traditional Brazilian Starch Biscuit.
34770809	1	13	contain	have	189:192	arg1	residue					141:147	The residue	137:147	The residue from commercial propolis extraction	137:183	The residue from commercial propolis extraction may have significant antioxidant power in food technology.
34770809	1	13	contain	have	189:192	arg2	power					218:222	significant antioxidant power	194:222	significant antioxidant power	194:222	The residue from commercial propolis extraction may have significant antioxidant power in food technology.
34770809	3	14	dep	days	565:568	arg1	co-product					709:718	lyophilized propolis co-product	688:718	lyophilized propolis co-product (LFCP)	688:725	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	14	dep	days	565:568	arg1	LFCP					721:724	LFCP	721:724	LFCP	721:724	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	14	dep	days	565:568	arg1	co-product					646:655	microencapsulated propolis co-product	619:655	microencapsulated propolis co-product (MECP) (with maltodextrin)	619:682	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	14	dep	days	565:568	arg1	days					565:568	45 days	562:568	45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP)	562:725	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	14	dep	days	565:568	arg1	prepared					609:616	prepared	609:616	were prepared	604:616	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	14	dep	days	565:568	arg1	MECP					658:661	MECP	658:661	MECP	658:661	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	15	theme	antioxidant	797:807	arg1	AA					819:820	AA	819:820	AA	819:820	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	15	theme	antioxidant	797:807	arg1	activity					809:816	antioxidant activity	797:816	antioxidant activity (AA)	797:821	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	7	16	theme	oleic	1156:1160	arg1	acids					1202:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids	1137:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids	1137:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids were found in biscuits at constant concentrations throughout the storage period.
34770809	7	17	theme	constant	1234:1241	arg1	concentrations					1243:1256	constant concentrations	1234:1256	constant concentrations	1234:1256	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids were found in biscuits at constant concentrations throughout the storage period.
34770809	5	18	theme	microencapsulation	924:941	arg1	process					943:949	The spray-drying microencapsulation process	907:949	The spray-drying microencapsulation process	907:949	The spray-drying microencapsulation process showed an efficiency of 63%.
34770809	1	19	from	extraction	174:183	arg1	residue					141:147	The residue	137:147	The residue from commercial propolis extraction	137:183	The residue from commercial propolis extraction may have significant antioxidant power in food technology.
34770809	3	20	theme	canola	536:541	arg1	oil					543:545	canola oil	536:545	canola oil	536:545	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	1	21	theme	significant	194:204	arg1	power					218:222	significant antioxidant power	194:222	significant antioxidant power	194:222	The residue from commercial propolis extraction may have significant antioxidant power in food technology.
34770809	9	22	theme	co-product	1430:1439	arg1	extracts					1441:1448	the propolis co-product extracts	1417:1448	the propolis co-product extracts	1417:1448	Therefore, the propolis co-product extracts could be utilized as a natural antioxidant to reduce lipid oxidation in fatty starch biscuit.
34770809	9	22	theme	co-product	1430:1439	arg1	antioxidant					1481:1491	a natural antioxidant	1471:1491	a natural antioxidant	1471:1491	Therefore, the propolis co-product extracts could be utilized as a natural antioxidant to reduce lipid oxidation in fatty starch biscuit.
34770809	3	23	theme	propolis	700:707	arg1	co-product					709:718	lyophilized propolis co-product	688:718	lyophilized propolis co-product (LFCP)	688:725	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	23	theme	propolis	700:707	arg1	days					565:568	45 days	562:568	45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP)	562:725	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	23	theme	propolis	700:707	arg1	LFCP					721:724	LFCP	721:724	LFCP	721:724	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	6	24	theme	chromatography	1112:1125	arg1	analysis					1127:1134	gas chromatography analysis	1108:1134	gas chromatography analysis	1108:1134	The LO in the biscuits was determined by the thiobarbituric acid reactive substances (TBARS) test and fatty acid composition by gas chromatography analysis.
34770809	9	25	theme	starch	1528:1533	arg1	biscuit					1535:1541	fatty starch biscuit	1522:1541	fatty starch biscuit	1522:1541	Therefore, the propolis co-product extracts could be utilized as a natural antioxidant to reduce lipid oxidation in fatty starch biscuit.
34770809	1	26	theme	antioxidant	206:216	arg1	power					218:222	significant antioxidant power	194:222	significant antioxidant power	194:222	The residue from commercial propolis extraction may have significant antioxidant power in food technology.
34770809	7	27	theme	stearic	1147:1153	arg1	acids					1202:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids	1137:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids	1137:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids were found in biscuits at constant concentrations throughout the storage period.
34770809	4	28	theme	Relevant	824:831	arg1	activity					845:852	Relevant antioxidant activity	824:852	Relevant antioxidant activity	824:852	Relevant antioxidant activity was observed using the methods of analysis employed.
34770809	2	29	from	goods	357:361	arg1	co-product					297:306	the propolis co-product	284:306	the propolis co-product	284:306	However, among the challenges for using the propolis co-product as an inhibitor of lipid oxidation (LO) in baked goods is maintaining its bioactive compounds.
34770809	2	29	from	goods	357:361	arg1	inhibitor					314:322	an inhibitor	311:322	an inhibitor of lipid oxidation (LO) in baked goods	311:361	However, among the challenges for using the propolis co-product as an inhibitor of lipid oxidation (LO) in baked goods is maintaining its bioactive compounds.
34770809	5	30	theme	spray-drying	911:922	arg1	process					943:949	The spray-drying microencapsulation process	907:949	The spray-drying microencapsulation process	907:949	The spray-drying microencapsulation process showed an efficiency of 63%.
34770809	6	31	theme	reactive	1045:1052	arg1	TBARS					1066:1070	TBARS	1066:1070	TBARS	1066:1070	The LO in the biscuits was determined by the thiobarbituric acid reactive substances (TBARS) test and fatty acid composition by gas chromatography analysis.
34770809	6	31	theme	reactive	1045:1052	arg1	substances					1054:1063	thiobarbituric acid reactive substances	1025:1063	the thiobarbituric acid reactive substances (TBARS) test	1021:1076	The LO in the biscuits was determined by the thiobarbituric acid reactive substances (TBARS) test and fatty acid composition by gas chromatography analysis.
34770809	0	32	theme	Microencapsulated	0:16	arg1	Co-Product					43:52	Microencapsulated and Lyophilized Propolis Co-Product	0:52	Microencapsulated and Lyophilized Propolis Co-Product	0:52	Microencapsulated and Lyophilized Propolis Co-Product Extract as Antioxidant Synthetic Replacer on Traditional Brazilian Starch Biscuit.
34770809	2	33	theme	oxidation	333:341	arg1	co-product					297:306	the propolis co-product	284:306	the propolis co-product	284:306	However, among the challenges for using the propolis co-product as an inhibitor of lipid oxidation (LO) in baked goods is maintaining its bioactive compounds.
34770809	2	33	theme	oxidation	333:341	arg1	inhibitor					314:322	an inhibitor	311:322	an inhibitor of lipid oxidation (LO) in baked goods	311:361	However, among the challenges for using the propolis co-product as an inhibitor of lipid oxidation (LO) in baked goods is maintaining its bioactive compounds.
34770809	2	34	theme	bioactive	382:390	arg1	compounds					392:400	its bioactive compounds	378:400	its bioactive compounds	378:400	However, among the challenges for using the propolis co-product as an inhibitor of lipid oxidation (LO) in baked goods is maintaining its bioactive compounds.
34770809	0	35	theme	Propolis	34:41	arg1	Co-Product					43:52	Microencapsulated and Lyophilized Propolis Co-Product	0:52	Microencapsulated and Lyophilized Propolis Co-Product	0:52	Microencapsulated and Lyophilized Propolis Co-Product Extract as Antioxidant Synthetic Replacer on Traditional Brazilian Starch Biscuit.
34770809	6	36	theme	substances	1054:1063	arg1	test					1073:1076	the thiobarbituric acid reactive substances (TBARS) test	1021:1076	the thiobarbituric acid reactive substances (TBARS) test	1021:1076	The LO in the biscuits was determined by the thiobarbituric acid reactive substances (TBARS) test and fatty acid composition by gas chromatography analysis.
34770809	9	37	theme	fatty	1522:1526	arg1	biscuit					1535:1541	fatty starch biscuit	1522:1541	fatty starch biscuit	1522:1541	Therefore, the propolis co-product extracts could be utilized as a natural antioxidant to reduce lipid oxidation in fatty starch biscuit.
34770809	7	38	from	concentrations	1243:1256	arg1	biscuits					1222:1229	biscuits	1222:1229	biscuits at constant concentrations	1222:1256	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids were found in biscuits at constant concentrations throughout the storage period.
34770809	2	39	theme	lipid	327:331	arg1	LO					344:345	LO	344:345	LO	344:345	However, among the challenges for using the propolis co-product as an inhibitor of lipid oxidation (LO) in baked goods is maintaining its bioactive compounds.
34770809	2	39	theme	lipid	327:331	arg1	oxidation					333:341	lipid oxidation	327:341	lipid oxidation (LO) in baked goods	327:361	However, among the challenges for using the propolis co-product as an inhibitor of lipid oxidation (LO) in baked goods is maintaining its bioactive compounds.
34770809	3	40	from	°C.	576:578	arg1	co-product					646:655	microencapsulated propolis co-product	619:655	microencapsulated propolis co-product (MECP) (with maltodextrin)	619:682	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	40	from	°C.	576:578	arg1	co-product					709:718	lyophilized propolis co-product	688:718	lyophilized propolis co-product (LFCP)	688:725	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	40	from	°C.	576:578	arg1	days					565:568	45 days	562:568	45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP)	562:725	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	1	41	theme	food	227:230	arg1	technology					232:241	food technology	227:241	food technology	227:241	The residue from commercial propolis extraction may have significant antioxidant power in food technology.
34770809	0	42	theme	Lyophilized	22:32	arg1	Co-Product					43:52	Microencapsulated and Lyophilized Propolis Co-Product	0:52	Microencapsulated and Lyophilized Propolis Co-Product	0:52	Microencapsulated and Lyophilized Propolis Co-Product Extract as Antioxidant Synthetic Replacer on Traditional Brazilian Starch Biscuit.
34770809	4	43	theme	analysis	888:895	arg1	methods					877:883	the methods	873:883	the methods of analysis employed	873:904	Relevant antioxidant activity was observed using the methods of analysis employed.
34770809	6	44	theme	gas	1108:1110	arg1	analysis					1127:1134	gas chromatography analysis	1108:1134	gas chromatography analysis	1108:1134	The LO in the biscuits was determined by the thiobarbituric acid reactive substances (TBARS) test and fatty acid composition by gas chromatography analysis.
34770809	3	45	with	co-product	646:655	arg1	maltodextrin					670:681	maltodextrin	670:681	maltodextrin	670:681	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	6	46	theme	acid	1040:1043	arg1	TBARS					1066:1070	TBARS	1066:1070	TBARS	1066:1070	The LO in the biscuits was determined by the thiobarbituric acid reactive substances (TBARS) test and fatty acid composition by gas chromatography analysis.
34770809	6	46	theme	acid	1040:1043	arg1	substances					1054:1063	thiobarbituric acid reactive substances	1025:1063	the thiobarbituric acid reactive substances (TBARS) test	1021:1076	The LO in the biscuits was determined by the thiobarbituric acid reactive substances (TBARS) test and fatty acid composition by gas chromatography analysis.
34770809	3	47	theme	co-product	457:466	arg1	extracts					468:475	propolis co-product extracts	448:475	the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP)	444:725	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	8	48	from	reduction	1314:1322	arg1	values					1343:1348	malondialdehyde values	1327:1348	malondialdehyde values	1327:1348	In addition, there was a reduction in malondialdehyde values with the addition of both propolis co-product extracts.
34770809	0	49	from	Replacer	87:94	arg1	Biscuit					128:134	Traditional Brazilian Starch Biscuit	99:134	Traditional Brazilian Starch Biscuit	99:134	Microencapsulated and Lyophilized Propolis Co-Product Extract as Antioxidant Synthetic Replacer on Traditional Brazilian Starch Biscuit.
34770809	3	50	theme	co-product	584:593	arg1	extracts					595:602	Two co-product extracts	580:602	Two co-product extracts	580:602	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	0	51	theme	Antioxidant	65:75	arg1	Replacer					87:94	Antioxidant Synthetic Replacer	65:94	Antioxidant Synthetic Replacer on Traditional Brazilian Starch Biscuit	65:134	Microencapsulated and Lyophilized Propolis Co-Product Extract as Antioxidant Synthetic Replacer on Traditional Brazilian Starch Biscuit.
34770809	7	52	theme	linoelaidic	1163:1173	arg1	acids					1202:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids	1137:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids	1137:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids were found in biscuits at constant concentrations throughout the storage period.
34770809	8	53	theme	malondialdehyde	1327:1341	arg1	values					1343:1348	malondialdehyde values	1327:1348	malondialdehyde values	1327:1348	In addition, there was a reduction in malondialdehyde values with the addition of both propolis co-product extracts.
34770809	3	54	theme	propolis	637:644	arg1	co-product					646:655	microencapsulated propolis co-product	619:655	microencapsulated propolis co-product (MECP) (with maltodextrin)	619:682	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	54	theme	propolis	637:644	arg1	days					565:568	45 days	562:568	45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP)	562:725	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	54	theme	propolis	637:644	arg1	MECP					658:661	MECP	658:661	MECP	658:661	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	2	55	theme	baked	351:355	arg1	goods					357:361	baked goods	351:361	baked goods	351:361	However, among the challenges for using the propolis co-product as an inhibitor of lipid oxidation (LO) in baked goods is maintaining its bioactive compounds.
34770809	9	56	theme	natural	1473:1479	arg1	extracts					1441:1448	the propolis co-product extracts	1417:1448	the propolis co-product extracts	1417:1448	Therefore, the propolis co-product extracts could be utilized as a natural antioxidant to reduce lipid oxidation in fatty starch biscuit.
34770809	9	56	theme	natural	1473:1479	arg1	antioxidant					1481:1491	a natural antioxidant	1471:1491	a natural antioxidant	1471:1491	Therefore, the propolis co-product extracts could be utilized as a natural antioxidant to reduce lipid oxidation in fatty starch biscuit.
34770809	3	57	dep	biscuit	512:518	arg1	stored					551:556	stored	551:556	stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP)	551:725	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	57	dep	biscuit	512:518	arg1	formulated					520:529	formulated	520:529	formulated with canola oil	520:545	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	58	theme	microencapsulated	619:635	arg1	co-product					646:655	microencapsulated propolis co-product	619:655	microencapsulated propolis co-product (MECP) (with maltodextrin)	619:682	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	58	theme	microencapsulated	619:635	arg1	days					565:568	45 days	562:568	45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP)	562:725	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	58	theme	microencapsulated	619:635	arg1	MECP					658:661	MECP	658:661	MECP	658:661	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	8	59	theme	co-product	1385:1394	arg1	extracts					1396:1403	both propolis co-product extracts	1371:1403	both propolis co-product extracts	1371:1403	In addition, there was a reduction in malondialdehyde values with the addition of both propolis co-product extracts.
34770809	7	60	theme	Palmitic	1137:1144	arg1	acids					1202:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids	1137:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids	1137:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids were found in biscuits at constant concentrations throughout the storage period.
34770809	5	61	theme	%	977:977	arg1	efficiency					961:970	an efficiency	958:970	an efficiency of 63%	958:977	The spray-drying microencapsulation process showed an efficiency of 63%.
34770809	2	62	theme	propolis	288:295	arg1	co-product					297:306	the propolis co-product	284:306	the propolis co-product	284:306	However, among the challenges for using the propolis co-product as an inhibitor of lipid oxidation (LO) in baked goods is maintaining its bioactive compounds.
34770809	2	62	theme	propolis	288:295	arg1	inhibitor					314:322	an inhibitor	311:322	an inhibitor of lipid oxidation (LO) in baked goods	311:361	However, among the challenges for using the propolis co-product as an inhibitor of lipid oxidation (LO) in baked goods is maintaining its bioactive compounds.
34770809	6	63	theme	acid	1088:1091	arg1	composition					1093:1103	fatty acid composition	1082:1103	fatty acid composition	1082:1103	The LO in the biscuits was determined by the thiobarbituric acid reactive substances (TBARS) test and fatty acid composition by gas chromatography analysis.
34770809	3	64	with	co-product	709:718	arg1	maltodextrin					670:681	maltodextrin	670:681	maltodextrin	670:681	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	3	65	theme	phenolic	776:783	arg1	content					785:791	their total phenolic content	764:791	their total phenolic content	764:791	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	9	66	theme	propolis	1421:1428	arg1	extracts					1441:1448	the propolis co-product extracts	1417:1448	the propolis co-product extracts	1417:1448	Therefore, the propolis co-product extracts could be utilized as a natural antioxidant to reduce lipid oxidation in fatty starch biscuit.
34770809	9	66	theme	propolis	1421:1428	arg1	antioxidant					1481:1491	a natural antioxidant	1471:1491	a natural antioxidant	1471:1491	Therefore, the propolis co-product extracts could be utilized as a natural antioxidant to reduce lipid oxidation in fatty starch biscuit.
34770809	8	67	theme	propolis	1376:1383	arg1	extracts					1396:1403	both propolis co-product extracts	1371:1403	both propolis co-product extracts	1371:1403	In addition, there was a reduction in malondialdehyde values with the addition of both propolis co-product extracts.
34770809	1	68	theme	commercial	154:163	arg1	extraction					174:183	commercial propolis extraction	154:183	commercial propolis extraction	154:183	The residue from commercial propolis extraction may have significant antioxidant power in food technology.
34770809	2	69	from	inhibitor	314:322	arg1	goods					357:361	baked goods	351:361	baked goods	351:361	However, among the challenges for using the propolis co-product as an inhibitor of lipid oxidation (LO) in baked goods is maintaining its bioactive compounds.
34770809	3	70	theme	content	785:791	arg1	analysis					752:759	analysis	752:759	analysis of their total phenolic content and antioxidant activity (AA)	752:821	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	7	71	theme	α-linolenic	1190:1200	arg1	acids					1202:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids	1137:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids	1137:1206	Palmitic, stearic, oleic, linoelaidic, linoleic, and α-linolenic acids were found in biscuits at constant concentrations throughout the storage period.
34770809	9	72	used	utilized	1459:1466	arg2	extracts					1441:1448	the propolis co-product extracts	1417:1448	the propolis co-product extracts	1417:1448	Therefore, the propolis co-product extracts could be utilized as a natural antioxidant to reduce lipid oxidation in fatty starch biscuit.
34770809	9	72	used	utilized	1459:1466	arg2	antioxidant					1481:1491	a natural antioxidant	1471:1491	a natural antioxidant	1471:1491	Therefore, the propolis co-product extracts could be utilized as a natural antioxidant to reduce lipid oxidation in fatty starch biscuit.
34770809	6	73	theme	thiobarbituric	1025:1038	arg1	TBARS					1066:1070	TBARS	1066:1070	TBARS	1066:1070	The LO in the biscuits was determined by the thiobarbituric acid reactive substances (TBARS) test and fatty acid composition by gas chromatography analysis.
34770809	6	73	theme	thiobarbituric	1025:1038	arg1	substances					1054:1063	thiobarbituric acid reactive substances	1025:1063	the thiobarbituric acid reactive substances (TBARS) test	1021:1076	The LO in the biscuits was determined by the thiobarbituric acid reactive substances (TBARS) test and fatty acid composition by gas chromatography analysis.
34770809	1	74	theme	propolis	165:172	arg1	extraction					174:183	commercial propolis extraction	154:183	commercial propolis extraction	154:183	The residue from commercial propolis extraction may have significant antioxidant power in food technology.
34770809	3	75	theme	propolis	448:455	arg1	extracts					468:475	propolis co-product extracts	448:475	the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP)	444:725	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
34770809	0	76	theme	Synthetic	77:85	arg1	Replacer					87:94	Antioxidant Synthetic Replacer	65:94	Antioxidant Synthetic Replacer on Traditional Brazilian Starch Biscuit	65:134	Microencapsulated and Lyophilized Propolis Co-Product Extract as Antioxidant Synthetic Replacer on Traditional Brazilian Starch Biscuit.
34770809	6	77	theme	fatty	1082:1086	arg1	composition					1093:1103	fatty acid composition	1082:1103	fatty acid composition	1082:1103	The LO in the biscuits was determined by the thiobarbituric acid reactive substances (TBARS) test and fatty acid composition by gas chromatography analysis.
34770809	6	78	from	LO	984:985	arg1	biscuits					994:1001	the biscuits	990:1001	the biscuits	990:1001	The LO in the biscuits was determined by the thiobarbituric acid reactive substances (TBARS) test and fatty acid composition by gas chromatography analysis.
34770809	8	79	with	reduction	1314:1322	arg1	addition					1359:1366	the addition	1355:1366	the addition of both propolis co-product extracts	1355:1403	In addition, there was a reduction in malondialdehyde values with the addition of both propolis co-product extracts.
34770809	3	80	theme	total	770:774	arg1	content					785:791	their total phenolic content	764:791	their total phenolic content	764:791	Therefore, this study aimed to determine the propolis co-product extracts' capability to reduce LO in starch biscuit formulated with canola oil and stored for 45 days at 25 °C. Two co-product extracts were prepared: microencapsulated propolis co-product (MECP) (with maltodextrin) and lyophilized propolis co-product (LFCP), which were subjected to analysis of their total phenolic content and antioxidant activity (AA).
33551170	6	0	theme	glycosylation	1079:1091	arg1	profiles					1093:1100	the N-linked glycosylation profiles	1066:1100	the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows	1066:1169	In the current study, we evaluated intercow differences and the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows.
33551170	10	1	theme	olein	1661:1665	arg1	infusion					1644:1651	Postruminal infusion	1632:1651	Postruminal infusion of palm olein, glucose, and essential AA	1632:1692	Postruminal infusion of palm olein, glucose, and essential AA resulted in shifts in the degree of GlyCAM-1 fucosylation within individual cows, ranging in some cases from 50 to 71% difference in degree of fucosylation, regardless of treatment.
33551170	0	2	link	N-linked	14:21	arg1	glycosylation					23:35	N-linked glycosylation	14:35	N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein	14:111	Variations in N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein: Intercow differences and dietary effects.
33551170	10	3	theme	AA	1691:1692	arg1	infusion					1644:1651	Postruminal infusion	1632:1651	Postruminal infusion of palm olein, glucose, and essential AA	1632:1692	Postruminal infusion of palm olein, glucose, and essential AA resulted in shifts in the degree of GlyCAM-1 fucosylation within individual cows, ranging in some cases from 50 to 71% difference in degree of fucosylation, regardless of treatment.
33551170	8	4	dep	73.3	1444:1447	arg1	to					1441:1442	to	1441:1442	to	1441:1442	The degree of fucosylation ranged from 44.8 to 73.3% between cows, and this variation was mainly attributed to the glycans of GlyCAM-1.
33551170	9	5	theme	milk	1607:1610	arg1	serum					1612:1616	the overall milk serum glycoprofile	1595:1629	the overall milk serum glycoprofile	1595:1629	Dietary supplementation with fat or protein did not influence the overall milk serum glycoprofile.
33551170	7	6	theme	N-linked	1375:1382	arg1	pool					1391:1394	the overall whey protein N-linked glycan pool	1350:1394	the overall whey protein N-linked glycan pool	1350:1394	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	10	7	theme	fucosylation	1837:1848	arg1	degree					1827:1832	degree	1827:1832	degree of fucosylation	1827:1848	Postruminal infusion of palm olein, glucose, and essential AA resulted in shifts in the degree of GlyCAM-1 fucosylation within individual cows, ranging in some cases from 50 to 71% difference in degree of fucosylation, regardless of treatment.
33551170	11	8	theme	nutrient	2073:2080	arg1	supply					2082:2087	nutrient supply	2073:2087	nutrient supply	2073:2087	Overall, these data demonstrate that the glycosylation, and particularly fucosylation, of GlyCAM-1 was variable, although these shifts appear to be related more to individual cow variation than to nutrient supply.
33551170	5	9	theme	GlyCAM-1	849:856	arg1	glycoprofile					833:844	glycoprofile	833:844	glycoprofile	833:844	We recently showed that the glycoprofile of GlyCAM-1 varies between cows and during early lactation, whereas the glycoprofile of lactoferrin was highly constant.
33551170	10	10	from	difference	1813:1822	arg1	degree					1827:1832	degree	1827:1832	degree of fucosylation	1827:1848	Postruminal infusion of palm olein, glucose, and essential AA resulted in shifts in the degree of GlyCAM-1 fucosylation within individual cows, ranging in some cases from 50 to 71% difference in degree of fucosylation, regardless of treatment.
33551170	9	11	theme	glycoprofile	1618:1629	arg1	serum					1612:1616	the overall milk serum glycoprofile	1595:1629	the overall milk serum glycoprofile	1595:1629	Dietary supplementation with fat or protein did not influence the overall milk serum glycoprofile.
33551170	7	12	theme	whey	1362:1365	arg1	pool					1391:1394	the overall whey protein N-linked glycan pool	1350:1394	the overall whey protein N-linked glycan pool	1350:1394	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	6	13	theme	milk	1132:1135	arg1	samples					1137:1143	milk samples	1132:1143	milk samples of Holstein-Friesian cows	1132:1169	In the current study, we evaluated intercow differences and the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows.
33551170	0	14	theme	Intercow	114:121	arg1	differences					123:133	Intercow differences	114:133	Intercow differences	114:133	Variations in N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein: Intercow differences and dietary effects.
33551170	6	15	theme	N-linked	1070:1077	arg1	profiles					1093:1100	the N-linked glycosylation profiles	1066:1100	the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows	1066:1169	In the current study, we evaluated intercow differences and the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows.
33551170	1	16	gly	glycoprotein	360:371	arg1	GlyCAM-1					326:333	GlyCAM-1	326:333	GlyCAM-1	326:333	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	1	16	gly	glycoprotein	360:371	arg1	glycoprotein					360:371	the dominant N-linked glycoprotein	338:371	the dominant N-linked glycoprotein in bovine whey protein products	338:403	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	7	17	theme	glycans	1339:1345	arg1	number					1329:1334	the total number	1319:1334	the total number of glycans in the overall whey protein N-linked glycan pool	1319:1394	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	1	18	theme	whey	383:386	arg1	products					396:403	bovine whey protein products	376:403	bovine whey protein products	376:403	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	10	19	theme	GlyCAM-1	1730:1737	arg1	fucosylation					1739:1750	GlyCAM-1 fucosylation	1730:1750	GlyCAM-1 fucosylation within individual cows	1730:1773	Postruminal infusion of palm olein, glucose, and essential AA resulted in shifts in the degree of GlyCAM-1 fucosylation within individual cows, ranging in some cases from 50 to 71% difference in degree of fucosylation, regardless of treatment.
33551170	7	20	from	%	1197:1197	arg1	protein					1228:1234	milk protein	1223:1234	milk protein	1223:1234	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	7	21	theme	number	1329:1334	arg1	%					1314:1314	approximately 78%	1298:1314	approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool	1298:1394	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	7	21	theme	number	1329:1334	arg1	number					1329:1334	the total number	1319:1334	the total number of glycans in the overall whey protein N-linked glycan pool	1319:1394	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	12	22	theme	mature	2189:2194	arg1	milk					2210:2213	mature, noncolostral milk	2189:2213	mature, noncolostral milk	2189:2213	To our knowledge, this is the first report of variation in glycosylation of a milk glycoprotein in mature, noncolostral milk.
33551170	4	23	dep	whether	710:716	arg1	modulated					794:802	modulated	794:802	can be modulated	787:802	It is therefore highly relevant to understand whether and how the glycosylation of whey proteins (and their functionality) can be modulated.
33551170	6	24	theme	intercow	1002:1009	arg1	differences					1011:1021	intercow differences	1002:1021	intercow differences	1002:1021	In the current study, we evaluated intercow differences and the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows.
33551170	2	25	theme	infant	480:485	arg1	formulas					487:494	infant formulas	480:494	infant formulas	480:494	Whey proteins are base ingredients in a range of food products, including infant formulas.
33551170	7	26	from	pool	1215:1218	arg1	protein					1228:1234	milk protein	1223:1234	milk protein	1223:1234	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	7	27	theme	pool	1215:1218	arg1	%					1197:1197	approximately 60%	1181:1197	approximately 60% of the N-glycan pool in milk protein	1181:1234	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	7	27	theme	pool	1215:1218	arg1	pool					1215:1218	the N-glycan pool	1202:1218	the N-glycan pool in milk protein	1202:1234	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	3	28	theme	glycans	606:612	arg1	interaction					591:601	the interaction	587:601	the interaction of glycans with the immune system via recognition receptors	587:661	Glycan monosaccharide composition and variation thereof may affect functionality, such as the interaction of glycans with the immune system via recognition receptors.
33551170	10	29	from	shifts	1706:1711	arg1	degree					1720:1725	the degree	1716:1725	the degree of GlyCAM-1 fucosylation within individual cows	1716:1773	Postruminal infusion of palm olein, glucose, and essential AA resulted in shifts in the degree of GlyCAM-1 fucosylation within individual cows, ranging in some cases from 50 to 71% difference in degree of fucosylation, regardless of treatment.
33551170	1	30	theme	bovine	159:164	arg1	serum					171:175	bovine milk serum	159:175	bovine milk serum	159:175	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	7	31	from	pool	1391:1394	arg1	number					1329:1334	the total number	1319:1334	the total number of glycans in the overall whey protein N-linked glycan pool	1319:1394	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	7	32	link	N-linked	1375:1382	arg1	pool					1391:1394	the overall whey protein N-linked glycan pool	1350:1394	the overall whey protein N-linked glycan pool	1350:1394	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	2	33	from	ingredients	429:439	arg1	range					446:450	a range	444:450	a range of food products, including infant formulas	444:494	Whey proteins are base ingredients in a range of food products, including infant formulas.
33551170	2	33	from	ingredients	429:439	arg1	formulas					487:494	infant formulas	480:494	infant formulas	480:494	Whey proteins are base ingredients in a range of food products, including infant formulas.
33551170	6	34	from	differences	1011:1021	arg1	profiles					1093:1100	the N-linked glycosylation profiles	1066:1100	the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows	1066:1169	In the current study, we evaluated intercow differences and the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows.
33551170	6	35	theme	Holstein-Friesian	1148:1164	arg1	cows					1166:1169	Holstein-Friesian cows	1148:1169	Holstein-Friesian cows	1148:1169	In the current study, we evaluated intercow differences and the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows.
33551170	12	36	theme	first	2120:2124	arg1	this					2108:2111	this	2108:2111	this	2108:2111	To our knowledge, this is the first report of variation in glycosylation of a milk glycoprotein in mature, noncolostral milk.
33551170	12	36	theme	first	2120:2124	arg1	report					2126:2131	the first report	2116:2131	the first report of variation in glycosylation of a milk glycoprotein in mature, noncolostral milk	2116:2213	To our knowledge, this is the first report of variation in glycosylation of a milk glycoprotein in mature, noncolostral milk.
33551170	4	37	gly	glycosylation	730:742	arg1	functionality					772:784	their functionality	766:784	their functionality	766:784	It is therefore highly relevant to understand whether and how the glycosylation of whey proteins (and their functionality) can be modulated.
33551170	4	37	gly	glycosylation	730:742	arg1	proteins					752:759	whey proteins	747:759	whey proteins	747:759	It is therefore highly relevant to understand whether and how the glycosylation of whey proteins (and their functionality) can be modulated.
33551170	12	38	theme	variation	2136:2144	arg1	this					2108:2111	this	2108:2111	this	2108:2111	To our knowledge, this is the first report of variation in glycosylation of a milk glycoprotein in mature, noncolostral milk.
33551170	12	38	theme	variation	2136:2144	arg1	report					2126:2131	the first report	2116:2131	the first report of variation in glycosylation of a milk glycoprotein in mature, noncolostral milk	2116:2213	To our knowledge, this is the first report of variation in glycosylation of a milk glycoprotein in mature, noncolostral milk.
33551170	0	39	theme	protein	105:111	arg1	glycosylation					23:35	N-linked glycosylation	14:35	N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein	14:111	Variations in N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein: Intercow differences and dietary effects.
33551170	1	40	theme	N-linked	351:358	arg1	GlyCAM-1					326:333	GlyCAM-1	326:333	GlyCAM-1	326:333	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	1	40	theme	N-linked	351:358	arg1	glycoprotein					360:371	the dominant N-linked glycoprotein	338:371	the dominant N-linked glycoprotein in bovine whey protein products	338:403	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	1	41	from	glycoprotein	360:371	arg1	products					396:403	bovine whey protein products	376:403	bovine whey protein products	376:403	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	11	42	theme	individual	2040:2049	arg1	variation					2055:2063	individual cow variation	2040:2063	individual cow variation than to nutrient supply	2040:2087	Overall, these data demonstrate that the glycosylation, and particularly fucosylation, of GlyCAM-1 was variable, although these shifts appear to be related more to individual cow variation than to nutrient supply.
33551170	7	43	from	number	1329:1334	arg1	pool					1391:1394	the overall whey protein N-linked glycan pool	1350:1394	the overall whey protein N-linked glycan pool	1350:1394	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	6	44	theme	macronutrient	1042:1054	arg1	supply					1056:1061	macronutrient supply	1042:1061	macronutrient supply	1042:1061	In the current study, we evaluated intercow differences and the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows.
33551170	2	45	theme	base	424:427	arg1	ingredients					429:439	base ingredients	424:439	base ingredients	424:439	Whey proteins are base ingredients in a range of food products, including infant formulas.
33551170	2	45	theme	base	424:427	arg1	proteins					411:418	Whey proteins	406:418	Whey proteins	406:418	Whey proteins are base ingredients in a range of food products, including infant formulas.
33551170	4	46	theme	whey	747:750	arg1	proteins					752:759	whey proteins	747:759	whey proteins	747:759	It is therefore highly relevant to understand whether and how the glycosylation of whey proteins (and their functionality) can be modulated.
33551170	0	47	theme	N-linked	14:21	arg1	glycosylation					23:35	N-linked glycosylation	14:35	N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein	14:111	Variations in N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein: Intercow differences and dietary effects.
33551170	1	48	theme	cellular	285:292	arg1	molecule					303:310	glycosylation-dependent cellular adhesion molecule 1	261:312	glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1)	261:323	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	1	48	theme	cellular	285:292	arg1	GlyCAM-1					315:322	GlyCAM-1	315:322	GlyCAM-1	315:322	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	6	49	theme	cows	1166:1169	arg1	samples					1137:1143	milk samples	1132:1143	milk samples of Holstein-Friesian cows	1132:1169	In the current study, we evaluated intercow differences and the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows.
33551170	4	50	dep	understand	699:708	arg1	whether					710:716	whether	710:716	whether	710:716	It is therefore highly relevant to understand whether and how the glycosylation of whey proteins (and their functionality) can be modulated.
33551170	7	51	from	glycans	1339:1345	arg1	pool					1391:1394	the overall whey protein N-linked glycan pool	1350:1394	the overall whey protein N-linked glycan pool	1350:1394	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	2	52	theme	food	455:458	arg1	products					460:467	food products	455:467	food products	455:467	Whey proteins are base ingredients in a range of food products, including infant formulas.
33551170	2	52	theme	food	455:458	arg1	formulas					487:494	infant formulas	480:494	infant formulas	480:494	Whey proteins are base ingredients in a range of food products, including infant formulas.
33551170	0	53	theme	molecule	78:85	arg1	protein					105:111	glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein	40:111	glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein	40:111	Variations in N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein: Intercow differences and dietary effects.
33551170	3	54	theme	recognition	641:651	arg1	receptors					653:661	recognition receptors	641:661	recognition receptors	641:661	Glycan monosaccharide composition and variation thereof may affect functionality, such as the interaction of glycans with the immune system via recognition receptors.
33551170	6	55	from	samples	1137:1143	arg1	profiles					1093:1100	the N-linked glycosylation profiles	1066:1100	the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows	1066:1169	In the current study, we evaluated intercow differences and the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows.
33551170	1	56	with	proteins	187:194	arg1	contribution					222:233	the highest N-glycan contribution	201:233	the highest N-glycan contribution	201:233	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	9	57	theme	Dietary	1533:1539	arg1	supplementation					1541:1555	Dietary supplementation	1533:1555	Dietary supplementation with fat or protein	1533:1575	Dietary supplementation with fat or protein did not influence the overall milk serum glycoprofile.
33551170	10	58	dep	%	1811:1811	arg1	71					1809:1810	71	1809:1810	71	1809:1810	Postruminal infusion of palm olein, glucose, and essential AA resulted in shifts in the degree of GlyCAM-1 fucosylation within individual cows, ranging in some cases from 50 to 71% difference in degree of fucosylation, regardless of treatment.
33551170	3	59	with	glycans	606:612	arg1	system					630:635	the immune system	619:635	the immune system	619:635	Glycan monosaccharide composition and variation thereof may affect functionality, such as the interaction of glycans with the immune system via recognition receptors.
33551170	10	60	theme	palm	1656:1659	arg1	olein					1661:1665	palm olein	1656:1665	palm olein	1656:1665	Postruminal infusion of palm olein, glucose, and essential AA resulted in shifts in the degree of GlyCAM-1 fucosylation within individual cows, ranging in some cases from 50 to 71% difference in degree of fucosylation, regardless of treatment.
33551170	0	61	theme	cell	64:67	arg1	molecule					78:85	glycosylation-dependent cell adhesion molecule 1	40:87	glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein	40:111	Variations in N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein: Intercow differences and dietary effects.
33551170	0	61	theme	cell	64:67	arg1	GlyCAM-1					90:97	GlyCAM-1	90:97	GlyCAM-1	90:97	Variations in N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein: Intercow differences and dietary effects.
33551170	0	62	gly	glycosylation	23:35	arg1	protein					105:111	glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein	40:111	glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein	40:111	Variations in N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein: Intercow differences and dietary effects.
33551170	6	63	theme	whey	1115:1118	arg1	proteins					1120:1127	the major whey proteins	1105:1127	the major whey proteins in milk samples of Holstein-Friesian cows	1105:1169	In the current study, we evaluated intercow differences and the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows.
33551170	6	64	link	N-linked	1070:1077	arg1	profiles					1093:1100	the N-linked glycosylation profiles	1066:1100	the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows	1066:1169	In the current study, we evaluated intercow differences and the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows.
33551170	1	65	theme	highest	205:211	arg1	contribution					222:233	the highest N-glycan contribution	201:233	the highest N-glycan contribution	201:233	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	7	66	gly	sialylated	1240:1249	arg1	%					1197:1197	approximately 60%	1181:1197	approximately 60% of the N-glycan pool in milk protein	1181:1234	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	7	66	gly	sialylated	1240:1249	arg1	pool					1215:1218	the N-glycan pool	1202:1218	the N-glycan pool in milk protein	1202:1234	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	13	67	theme	variable	2247:2254	arg1	fucosylation					2265:2276	variable GlyCAM-1 fucosylation	2247:2276	variable GlyCAM-1 fucosylation	2247:2276	The functional implications of variable GlyCAM-1 fucosylation remain to be investigated.
33551170	3	68	theme	Glycan	497:502	arg1	composition					519:529	Glycan monosaccharide composition	497:529	Glycan monosaccharide composition	497:529	Glycan monosaccharide composition and variation thereof may affect functionality, such as the interaction of glycans with the immune system via recognition receptors.
33551170	0	69	dep	Variations	0:9	arg1	differences					123:133	Intercow differences	114:133	Intercow differences	114:133	Variations in N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein: Intercow differences and dietary effects.
33551170	0	69	dep	Variations	0:9	arg1	effects					147:153	dietary effects	139:153	dietary effects	139:153	Variations in N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein: Intercow differences and dietary effects.
33551170	6	70	from	profiles	1093:1100	arg1	samples					1137:1143	milk samples	1132:1143	milk samples of Holstein-Friesian cows	1132:1169	In the current study, we evaluated intercow differences and the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows.
33551170	7	71	theme	milk	1223:1226	arg1	protein					1228:1234	milk protein	1223:1234	milk protein	1223:1234	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	11	72	gly	glycosylation	1917:1929	arg1	GlyCAM-1					1966:1973	GlyCAM-1	1966:1973	GlyCAM-1	1966:1973	Overall, these data demonstrate that the glycosylation, and particularly fucosylation, of GlyCAM-1 was variable, although these shifts appear to be related more to individual cow variation than to nutrient supply.
33551170	9	73	with	supplementation	1541:1555	arg1	protein					1569:1575	protein	1569:1575	protein	1569:1575	Dietary supplementation with fat or protein did not influence the overall milk serum glycoprofile.
33551170	9	73	with	supplementation	1541:1555	arg1	fat					1562:1564	fat	1562:1564	fat	1562:1564	Dietary supplementation with fat or protein did not influence the overall milk serum glycoprofile.
33551170	1	74	from	molecule	303:310	arg1	serum					171:175	bovine milk serum	159:175	bovine milk serum	159:175	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	9	75	theme	overall	1599:1605	arg1	serum					1612:1616	the overall milk serum glycoprofile	1595:1629	the overall milk serum glycoprofile	1595:1629	Dietary supplementation with fat or protein did not influence the overall milk serum glycoprofile.
33551170	7	76	from	protein	1228:1234	arg1	%					1197:1197	approximately 60%	1181:1197	approximately 60% of the N-glycan pool in milk protein	1181:1234	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	7	76	from	protein	1228:1234	arg1	pool					1215:1218	the N-glycan pool	1202:1218	the N-glycan pool in milk protein	1202:1234	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	10	77	theme	essential	1681:1689	arg1	AA					1691:1692	essential AA	1681:1692	essential AA	1681:1692	Postruminal infusion of palm olein, glucose, and essential AA resulted in shifts in the degree of GlyCAM-1 fucosylation within individual cows, ranging in some cases from 50 to 71% difference in degree of fucosylation, regardless of treatment.
33551170	12	78	from	report	2126:2131	arg1	glycosylation					2149:2161	glycosylation	2149:2161	glycosylation of a milk glycoprotein in mature, noncolostral milk	2149:2213	To our knowledge, this is the first report of variation in glycosylation of a milk glycoprotein in mature, noncolostral milk.
33551170	1	79	theme	milk	166:169	arg1	serum					171:175	bovine milk serum	159:175	bovine milk serum	159:175	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	11	80	theme	cow	2051:2053	arg1	variation					2055:2063	individual cow variation	2040:2063	individual cow variation than to nutrient supply	2040:2087	Overall, these data demonstrate that the glycosylation, and particularly fucosylation, of GlyCAM-1 was variable, although these shifts appear to be related more to individual cow variation than to nutrient supply.
33551170	7	81	theme	glycan	1384:1389	arg1	pool					1391:1394	the overall whey protein N-linked glycan pool	1350:1394	the overall whey protein N-linked glycan pool	1350:1394	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	13	82	theme	functional	2220:2229	arg1	implications					2231:2242	The functional implications	2216:2242	The functional implications of variable GlyCAM-1 fucosylation	2216:2276	The functional implications of variable GlyCAM-1 fucosylation remain to be investigated.
33551170	7	83	theme	protein	1367:1373	arg1	pool					1391:1394	the overall whey protein N-linked glycan pool	1350:1394	the overall whey protein N-linked glycan pool	1350:1394	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	1	84	from	IgG	252:254	arg1	serum					171:175	bovine milk serum	159:175	bovine milk serum	159:175	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	6	85	from	effects	1031:1037	arg1	profiles					1093:1100	the N-linked glycosylation profiles	1066:1100	the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows	1066:1169	In the current study, we evaluated intercow differences and the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows.
33551170	1	86	theme	whey	182:185	arg1	proteins					187:194	the whey proteins	178:194	the whey proteins with the highest N-glycan contribution	178:233	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	1	86	theme	whey	182:185	arg1	lactoferrin					239:249	lactoferrin	239:249	lactoferrin	239:249	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	7	87	theme	overall	1354:1360	arg1	pool					1391:1394	the overall whey protein N-linked glycan pool	1350:1394	the overall whey protein N-linked glycan pool	1350:1394	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	1	88	from	lactoferrin	239:249	arg1	serum					171:175	bovine milk serum	159:175	bovine milk serum	159:175	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	1	89	theme	bovine	376:381	arg1	products					396:403	bovine whey protein products	376:403	bovine whey protein products	376:403	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	10	90	theme	fucosylation	1739:1750	arg1	degree					1720:1725	the degree	1716:1725	the degree of GlyCAM-1 fucosylation within individual cows	1716:1773	Postruminal infusion of palm olein, glucose, and essential AA resulted in shifts in the degree of GlyCAM-1 fucosylation within individual cows, ranging in some cases from 50 to 71% difference in degree of fucosylation, regardless of treatment.
33551170	12	91	theme	glycoprotein	2173:2184	arg1	glycosylation					2149:2161	glycosylation	2149:2161	glycosylation of a milk glycoprotein in mature, noncolostral milk	2149:2213	To our knowledge, this is the first report of variation in glycosylation of a milk glycoprotein in mature, noncolostral milk.
33551170	11	92	theme	GlyCAM-1	1966:1973	arg1	glycosylation					1917:1929	glycosylation	1917:1929	glycosylation	1917:1929	Overall, these data demonstrate that the glycosylation, and particularly fucosylation, of GlyCAM-1 was variable, although these shifts appear to be related more to individual cow variation than to nutrient supply.
33551170	11	92	theme	GlyCAM-1	1966:1973	arg1	variable					1979:1986	variable	1979:1986	variable	1979:1986	Overall, these data demonstrate that the glycosylation, and particularly fucosylation, of GlyCAM-1 was variable, although these shifts appear to be related more to individual cow variation than to nutrient supply.
33551170	8	93	theme	fucosylation	1411:1422	arg1	degree					1401:1406	The degree	1397:1406	The degree of fucosylation	1397:1422	The degree of fucosylation ranged from 44.8 to 73.3% between cows, and this variation was mainly attributed to the glycans of GlyCAM-1.
33551170	1	94	theme	protein	388:394	arg1	products					396:403	bovine whey protein products	376:403	bovine whey protein products	376:403	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	0	95	from	Variations	0:9	arg1	glycosylation					23:35	N-linked glycosylation	14:35	N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein	14:111	Variations in N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein: Intercow differences and dietary effects.
33551170	12	96	gly	glycoprotein	2173:2184	arg1	glycoprotein					2173:2184	a milk glycoprotein	2166:2184	a milk glycoprotein	2166:2184	To our knowledge, this is the first report of variation in glycosylation of a milk glycoprotein in mature, noncolostral milk.
33551170	7	97	theme	total	1323:1327	arg1	number					1329:1334	the total number	1319:1334	the total number of glycans in the overall whey protein N-linked glycan pool	1319:1394	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	7	98	theme	N-glycan	1206:1213	arg1	pool					1215:1218	the N-glycan pool	1202:1218	the N-glycan pool in milk protein	1202:1234	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	10	99	theme	individual	1759:1768	arg1	cows					1770:1773	individual cows	1759:1773	individual cows	1759:1773	Postruminal infusion of palm olein, glucose, and essential AA resulted in shifts in the degree of GlyCAM-1 fucosylation within individual cows, ranging in some cases from 50 to 71% difference in degree of fucosylation, regardless of treatment.
33551170	12	100	theme	milk	2168:2171	arg1	glycoprotein					2173:2184	a milk glycoprotein	2166:2184	a milk glycoprotein	2166:2184	To our knowledge, this is the first report of variation in glycosylation of a milk glycoprotein in mature, noncolostral milk.
33551170	12	101	from	glycosylation	2149:2161	arg1	this					2108:2111	this	2108:2111	this	2108:2111	To our knowledge, this is the first report of variation in glycosylation of a milk glycoprotein in mature, noncolostral milk.
33551170	12	101	from	glycosylation	2149:2161	arg1	milk					2210:2213	mature, noncolostral milk	2189:2213	mature, noncolostral milk	2189:2213	To our knowledge, this is the first report of variation in glycosylation of a milk glycoprotein in mature, noncolostral milk.
33551170	12	101	from	glycosylation	2149:2161	arg1	report					2126:2131	the first report	2116:2131	the first report of variation in glycosylation of a milk glycoprotein in mature, noncolostral milk	2116:2213	To our knowledge, this is the first report of variation in glycosylation of a milk glycoprotein in mature, noncolostral milk.
33551170	12	102	gly	glycosylation	2149:2161	arg1	milk					2210:2213	mature, noncolostral milk	2189:2213	mature, noncolostral milk	2189:2213	To our knowledge, this is the first report of variation in glycosylation of a milk glycoprotein in mature, noncolostral milk.
33551170	12	102	gly	glycosylation	2149:2161	arg1	glycoprotein					2173:2184	a milk glycoprotein	2166:2184	a milk glycoprotein	2166:2184	To our knowledge, this is the first report of variation in glycosylation of a milk glycoprotein in mature, noncolostral milk.
33551170	2	103	theme	products	460:467	arg1	range					446:450	a range	444:450	a range of food products, including infant formulas	444:494	Whey proteins are base ingredients in a range of food products, including infant formulas.
33551170	2	103	theme	products	460:467	arg1	formulas					487:494	infant formulas	480:494	infant formulas	480:494	Whey proteins are base ingredients in a range of food products, including infant formulas.
33551170	0	104	theme	whey	100:103	arg1	protein					105:111	glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein	40:111	glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein	40:111	Variations in N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein: Intercow differences and dietary effects.
33551170	6	105	theme	current	974:980	arg1	study					982:986	the current study	970:986	the current study	970:986	In the current study, we evaluated intercow differences and the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows.
33551170	6	106	gly	glycosylation	1079:1091	arg1	proteins					1120:1127	the major whey proteins	1105:1127	the major whey proteins in milk samples of Holstein-Friesian cows	1105:1169	In the current study, we evaluated intercow differences and the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows.
33551170	4	107	theme	proteins	752:759	arg1	glycosylation					730:742	the glycosylation	726:742	the glycosylation of whey proteins (and their functionality)	726:785	It is therefore highly relevant to understand whether and how the glycosylation of whey proteins (and their functionality) can be modulated.
33551170	1	108	link	N-linked	351:358	arg1	GlyCAM-1					326:333	GlyCAM-1	326:333	GlyCAM-1	326:333	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	1	108	link	N-linked	351:358	arg1	glycoprotein					360:371	the dominant N-linked glycoprotein	338:371	the dominant N-linked glycoprotein in bovine whey protein products	338:403	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	10	109	theme	Postruminal	1632:1642	arg1	infusion					1644:1651	Postruminal infusion	1632:1651	Postruminal infusion of palm olein, glucose, and essential AA	1632:1692	Postruminal infusion of palm olein, glucose, and essential AA resulted in shifts in the degree of GlyCAM-1 fucosylation within individual cows, ranging in some cases from 50 to 71% difference in degree of fucosylation, regardless of treatment.
33551170	1	110	theme	dominant	342:349	arg1	GlyCAM-1					326:333	GlyCAM-1	326:333	GlyCAM-1	326:333	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	1	110	theme	dominant	342:349	arg1	glycoprotein					360:371	the dominant N-linked glycoprotein	338:371	the dominant N-linked glycoprotein in bovine whey protein products	338:403	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	6	111	theme	supply	1056:1061	arg1	effects					1031:1037	the effects	1027:1037	the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows	1027:1169	In the current study, we evaluated intercow differences and the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows.
33551170	6	111	theme	supply	1056:1061	arg1	differences					1011:1021	intercow differences	1002:1021	intercow differences	1002:1021	In the current study, we evaluated intercow differences and the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows.
33551170	0	112	theme	dietary	139:145	arg1	effects					147:153	dietary effects	139:153	dietary effects	139:153	Variations in N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein: Intercow differences and dietary effects.
33551170	5	113	theme	early	889:893	arg1	lactation					895:903	early lactation	889:903	early lactation	889:903	We recently showed that the glycoprofile of GlyCAM-1 varies between cows and during early lactation, whereas the glycoprofile of lactoferrin was highly constant.
33551170	1	114	theme	glycosylation-dependent	261:283	arg1	molecule					303:310	glycosylation-dependent cellular adhesion molecule 1	261:312	glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1)	261:323	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	1	114	theme	glycosylation-dependent	261:283	arg1	GlyCAM-1					315:322	GlyCAM-1	315:322	GlyCAM-1	315:322	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	5	115	theme	lactoferrin	934:944	arg1	constant					957:964	constant	957:964	constant	957:964	We recently showed that the glycoprofile of GlyCAM-1 varies between cows and during early lactation, whereas the glycoprofile of lactoferrin was highly constant.
33551170	5	115	theme	lactoferrin	934:944	arg1	glycoprofile					918:929	glycoprofile	918:929	glycoprofile	918:929	We recently showed that the glycoprofile of GlyCAM-1 varies between cows and during early lactation, whereas the glycoprofile of lactoferrin was highly constant.
33551170	2	116	theme	Whey	406:409	arg1	ingredients					429:439	base ingredients	424:439	base ingredients	424:439	Whey proteins are base ingredients in a range of food products, including infant formulas.
33551170	2	116	theme	Whey	406:409	arg1	proteins					411:418	Whey proteins	406:418	Whey proteins	406:418	Whey proteins are base ingredients in a range of food products, including infant formulas.
33551170	0	117	theme	glycosylation-dependent	40:62	arg1	molecule					78:85	glycosylation-dependent cell adhesion molecule 1	40:87	glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein	40:111	Variations in N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein: Intercow differences and dietary effects.
33551170	0	117	theme	glycosylation-dependent	40:62	arg1	GlyCAM-1					90:97	GlyCAM-1	90:97	GlyCAM-1	90:97	Variations in N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein: Intercow differences and dietary effects.
33551170	1	118	theme	adhesion	294:301	arg1	molecule					303:310	glycosylation-dependent cellular adhesion molecule 1	261:312	glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1)	261:323	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	1	118	theme	adhesion	294:301	arg1	GlyCAM-1					315:322	GlyCAM-1	315:322	GlyCAM-1	315:322	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	10	119	dep	difference	1813:1822	arg1	%					1811:1811	%	1811:1811	%	1811:1811	Postruminal infusion of palm olein, glucose, and essential AA resulted in shifts in the degree of GlyCAM-1 fucosylation within individual cows, ranging in some cases from 50 to 71% difference in degree of fucosylation, regardless of treatment.
33551170	3	120	theme	immune	623:628	arg1	system					630:635	the immune system	619:635	the immune system	619:635	Glycan monosaccharide composition and variation thereof may affect functionality, such as the interaction of glycans with the immune system via recognition receptors.
33551170	12	121	from	variation	2136:2144	arg1	glycosylation					2149:2161	glycosylation	2149:2161	glycosylation of a milk glycoprotein in mature, noncolostral milk	2149:2213	To our knowledge, this is the first report of variation in glycosylation of a milk glycoprotein in mature, noncolostral milk.
33551170	0	122	theme	adhesion	69:76	arg1	molecule					78:85	glycosylation-dependent cell adhesion molecule 1	40:87	glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein	40:111	Variations in N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein: Intercow differences and dietary effects.
33551170	0	122	theme	adhesion	69:76	arg1	GlyCAM-1					90:97	GlyCAM-1	90:97	GlyCAM-1	90:97	Variations in N-linked glycosylation of glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) whey protein: Intercow differences and dietary effects.
33551170	7	123	gly	fucosylated	1255:1265	arg1	%					1197:1197	approximately 60%	1181:1197	approximately 60% of the N-glycan pool in milk protein	1181:1234	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	7	123	gly	fucosylated	1255:1265	arg1	pool					1215:1218	the N-glycan pool	1202:1218	the N-glycan pool in milk protein	1202:1234	Overall, approximately 60% of the N-glycan pool in milk protein was sialylated, or fucosylated, or both; GlyCAM-1 contributed approximately 78% of the total number of glycans in the overall whey protein N-linked glycan pool.
33551170	6	124	theme	proteins	1120:1127	arg1	profiles					1093:1100	the N-linked glycosylation profiles	1066:1100	the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows	1066:1169	In the current study, we evaluated intercow differences and the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows.
33551170	8	125	theme	GlyCAM-1	1523:1530	arg1	glycans					1512:1518	the glycans	1508:1518	the glycans of GlyCAM-1	1508:1530	The degree of fucosylation ranged from 44.8 to 73.3% between cows, and this variation was mainly attributed to the glycans of GlyCAM-1.
33551170	6	126	from	proteins	1120:1127	arg1	samples					1137:1143	milk samples	1132:1143	milk samples of Holstein-Friesian cows	1132:1169	In the current study, we evaluated intercow differences and the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows.
33551170	13	127	theme	fucosylation	2265:2276	arg1	implications					2231:2242	The functional implications	2216:2242	The functional implications of variable GlyCAM-1 fucosylation	2216:2276	The functional implications of variable GlyCAM-1 fucosylation remain to be investigated.
33551170	6	128	theme	major	1109:1113	arg1	proteins					1120:1127	the major whey proteins	1105:1127	the major whey proteins in milk samples of Holstein-Friesian cows	1105:1169	In the current study, we evaluated intercow differences and the effects of macronutrient supply on the N-linked glycosylation profiles of the major whey proteins in milk samples of Holstein-Friesian cows.
33551170	4	129	theme	functionality	772:784	arg1	glycosylation					730:742	the glycosylation	726:742	the glycosylation of whey proteins (and their functionality)	726:785	It is therefore highly relevant to understand whether and how the glycosylation of whey proteins (and their functionality) can be modulated.
33551170	10	130	theme	glucose	1668:1674	arg1	infusion					1644:1651	Postruminal infusion	1632:1651	Postruminal infusion of palm olein, glucose, and essential AA	1632:1692	Postruminal infusion of palm olein, glucose, and essential AA resulted in shifts in the degree of GlyCAM-1 fucosylation within individual cows, ranging in some cases from 50 to 71% difference in degree of fucosylation, regardless of treatment.
33551170	1	131	theme	N-glycan	213:220	arg1	contribution					222:233	the highest N-glycan contribution	201:233	the highest N-glycan contribution	201:233	In bovine milk serum, the whey proteins with the highest N-glycan contribution are lactoferrin, IgG, and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1); GlyCAM-1 is the dominant N-linked glycoprotein in bovine whey protein products.
33551170	13	132	theme	GlyCAM-1	2256:2263	arg1	fucosylation					2265:2276	variable GlyCAM-1 fucosylation	2247:2276	variable GlyCAM-1 fucosylation	2247:2276	The functional implications of variable GlyCAM-1 fucosylation remain to be investigated.
33551170	10	133	dep	71	1809:1810	arg1	to					1806:1807	to	1806:1807	to	1806:1807	Postruminal infusion of palm olein, glucose, and essential AA resulted in shifts in the degree of GlyCAM-1 fucosylation within individual cows, ranging in some cases from 50 to 71% difference in degree of fucosylation, regardless of treatment.
33551170	3	134	theme	monosaccharide	504:517	arg1	composition					519:529	Glycan monosaccharide composition	497:529	Glycan monosaccharide composition	497:529	Glycan monosaccharide composition and variation thereof may affect functionality, such as the interaction of glycans with the immune system via recognition receptors.
33551170	12	135	dep	mature	2189:2194	arg1	noncolostral					2197:2208	noncolostral	2197:2208	noncolostral	2197:2208	To our knowledge, this is the first report of variation in glycosylation of a milk glycoprotein in mature, noncolostral milk.
33626372	6	0	theme	in	1027:1028	arg1	condition					1036:1044	in vitro condition	1027:1044	in vitro condition	1027:1044	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	11	1	theme	high	1727:1730	arg1	chromatography					1766:1779	high performance anion exchange (HPAE) chromatography	1727:1779	high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system	1727:1835	Presence of carbohydrate polymers such as rhamnose, arabinose, galactose, glucose and xylose revealed using high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system were measured using Pulsed Amperometric Detection (PAD).
33626372	6	2	theme	therapeutic	908:918	arg1	applications					920:931	therapeutic applications	908:931	therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities)	908:1019	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	6	2	theme	therapeutic	908:918	arg1	activities					1009:1018	antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities	934:1018	antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities	934:1018	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	10	3	theme	flower	1445:1450	arg1	extract					1452:1458	tamarind flower extract	1436:1458	tamarind flower extract	1436:1458	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	9	4	theme	Cl	1276:1277	arg1	presence					1251:1258	the presence	1247:1258	the presence of O, Na, P and Cl	1247:1277	EDX analysis exhibited the presence of O, Na, P and Cl, while DLS confirmed bilayer formation of xyloglucan and liposomes with uniform size (70-80 nm) and spherical shape.
33626372	5	5	used	used	726:729	arg2	molecules					683:691	The biological molecules	668:691	The biological molecules of Tamarindus indica flower	668:719	The biological molecules of Tamarindus indica flower were used for the synthesis of nanoliposome.
33626372	6	6	theme	anti-inflammatory	991:1007	arg1	applications					920:931	therapeutic applications	908:931	therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities)	908:1019	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	6	6	theme	anti-inflammatory	991:1007	arg1	activities					1009:1018	antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities	934:1018	antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities	934:1018	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	13	7	dep	in	2058:2059	arg1	vitro					2061:2065	vitro	2061:2065	vitro	2061:2065	TifeLiNPs demonstrated significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity.
33626372	12	8	theme	antibacterial	1999:2011	arg1	activity					2013:2020	potential antibacterial activity	1989:2020	potential antibacterial activity	1989:2020	The synthesized nanoliposome evaluated against Gram negative and Gram positive bacteria showed potential antibacterial activity.
33626372	5	9	theme	Tamarindus	696:705	arg1	flower					714:719	Tamarindus indica flower	696:719	Tamarindus indica flower	696:719	The biological molecules of Tamarindus indica flower were used for the synthesis of nanoliposome.
33626372	13	10	theme	potential	2079:2087	arg1	activity					2138:2145	significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity	2046:2145	significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity	2046:2145	TifeLiNPs demonstrated significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity.
33626372	6	11	theme	anticancer	976:985	arg1	applications					920:931	therapeutic applications	908:931	therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities)	908:1019	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	6	11	theme	anticancer	976:985	arg1	activities					1009:1018	antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities	934:1018	antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities	934:1018	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	10	12	theme	glycosides	1552:1561	arg1	presence					1502:1509	the presence	1498:1509	the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test	1498:1616	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	13	13	theme	antidiabetic	2090:2101	arg1	activity					2138:2145	significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity	2046:2145	significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity	2046:2145	TifeLiNPs demonstrated significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity.
33626372	2	14	theme	application	347:357	arg1	areas					359:363	various application areas	339:363	various application areas	339:363	Tamarind flower extract is rich in Xyloglucan, a starch like polysaccharide which promotes proliferation and various application areas like drug-delivery technology.
33626372	9	15	theme	P	1270:1270	arg1	presence					1251:1258	the presence	1247:1258	the presence of O, Na, P and Cl	1247:1277	EDX analysis exhibited the presence of O, Na, P and Cl, while DLS confirmed bilayer formation of xyloglucan and liposomes with uniform size (70-80 nm) and spherical shape.
33626372	9	16	theme	xyloglucan	1321:1330	arg1	formation					1308:1316	bilayer formation	1300:1316	bilayer formation of xyloglucan and liposomes	1300:1344	EDX analysis exhibited the presence of O, Na, P and Cl, while DLS confirmed bilayer formation of xyloglucan and liposomes with uniform size (70-80 nm) and spherical shape.
33626372	8	17	from	presence	1176:1183	arg1	group					1217:1221	the functional group	1202:1221	the functional group	1202:1221	Further, the chemical characterization using FTIR revealed the presence of components in the functional group.
33626372	9	18	theme	liposomes	1336:1344	arg1	formation					1308:1316	bilayer formation	1300:1316	bilayer formation of xyloglucan and liposomes	1300:1344	EDX analysis exhibited the presence of O, Na, P and Cl, while DLS confirmed bilayer formation of xyloglucan and liposomes with uniform size (70-80 nm) and spherical shape.
33626372	1	19	theme	interesting	138:148	arg1	area					150:153	interesting area	138:153	interesting area of research developed for several diagnostic and therapeutic applications	138:227	Nanoparticles are interesting area of research developed for several diagnostic and therapeutic applications.
33626372	1	19	theme	interesting	138:148	arg1	Nanoparticles					120:132	Nanoparticles	120:132	Nanoparticles	120:132	Nanoparticles are interesting area of research developed for several diagnostic and therapeutic applications.
33626372	13	20	theme	in	2058:2059	arg1	activity					2138:2145	significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity	2046:2145	significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity	2046:2145	TifeLiNPs demonstrated significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity.
33626372	9	21	theme	uniform	1351:1357	arg1	70-80 nm					1365:1372	70-80 nm	1365:1372	70-80 nm	1365:1372	EDX analysis exhibited the presence of O, Na, P and Cl, while DLS confirmed bilayer formation of xyloglucan and liposomes with uniform size (70-80 nm) and spherical shape.
33626372	9	21	theme	uniform	1351:1357	arg1	size					1359:1362	uniform size	1351:1362	uniform size (70-80 nm)	1351:1373	EDX analysis exhibited the presence of O, Na, P and Cl, while DLS confirmed bilayer formation of xyloglucan and liposomes with uniform size (70-80 nm) and spherical shape.
33626372	13	22	theme	antioxidant	2067:2077	arg1	activity					2138:2145	significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity	2046:2145	significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity	2046:2145	TifeLiNPs demonstrated significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity.
33626372	10	23	attach	presence	1502:1509	arg1	test					1613:1616	confirmatory test	1600:1616	confirmatory test	1600:1616	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	10	23	attach	presence	1502:1509	arg2	carbohydrates					1514:1526	carbohydrates	1514:1526	carbohydrates	1514:1526	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	10	23	attach	presence	1502:1509	arg2	glycosides					1552:1561	glycosides	1552:1561	glycosides	1552:1561	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	10	23	attach	presence	1502:1509	arg2	tannins					1574:1580	tannins	1574:1580	tannins	1574:1580	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	10	23	attach	presence	1502:1509	arg2	saponins					1564:1571	saponins	1564:1571	saponins	1564:1571	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	10	23	attach	presence	1502:1509	arg2	flavonoids					1586:1595	flavonoids	1586:1595	flavonoids	1586:1595	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	10	23	attach	presence	1502:1509	arg2	alkaloids					1529:1537	alkaloids	1529:1537	alkaloids	1529:1537	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	10	23	attach	presence	1502:1509	arg2	terpenoids					1540:1549	terpenoids	1540:1549	terpenoids	1540:1549	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	0	24	theme	Xyloglucans	60:70	arg1	polysaccharides					72:86	Xyloglucans polysaccharides	60:86	Xyloglucans polysaccharides from Tamarindus flower extract	60:117	In vitro therapeutic evaluation of nanoliposome loaded with Xyloglucans polysaccharides from Tamarindus flower extract.
33626372	8	25	theme	chemical	1126:1133	arg1	characterization					1135:1150	the chemical characterization	1122:1150	the chemical characterization using FTIR	1122:1161	Further, the chemical characterization using FTIR revealed the presence of components in the functional group.
33626372	1	26	theme	several	181:187	arg1	applications					216:227	several diagnostic and therapeutic applications	181:227	several diagnostic and therapeutic applications	181:227	Nanoparticles are interesting area of research developed for several diagnostic and therapeutic applications.
33626372	4	27	theme	nanoliposome	576:587	arg1	importance					546:555	importance	546:555	importance	546:555	Considering the importance and feasibility of nanoliposome, the present study is focused on synthesis of liposomes via biological method.
33626372	4	27	theme	nanoliposome	576:587	arg1	feasibility					561:571	feasibility	561:571	feasibility	561:571	Considering the importance and feasibility of nanoliposome, the present study is focused on synthesis of liposomes via biological method.
33626372	13	28	theme	anti-inflammatory	2120:2136	arg1	activity					2138:2145	significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity	2046:2145	significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity	2046:2145	TifeLiNPs demonstrated significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity.
33626372	11	29	theme	Amperometric	1864:1875	arg1	Detection					1877:1885	Pulsed Amperometric Detection	1857:1885	Pulsed Amperometric Detection (PAD)	1857:1891	Presence of carbohydrate polymers such as rhamnose, arabinose, galactose, glucose and xylose revealed using high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system were measured using Pulsed Amperometric Detection (PAD).
33626372	11	29	theme	Amperometric	1864:1875	arg1	PAD					1888:1890	PAD	1888:1890	PAD	1888:1890	Presence of carbohydrate polymers such as rhamnose, arabinose, galactose, glucose and xylose revealed using high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system were measured using Pulsed Amperometric Detection (PAD).
33626372	0	30	theme	flower	104:109	arg1	extract					111:117	Tamarindus flower extract	93:117	Tamarindus flower extract	93:117	In vitro therapeutic evaluation of nanoliposome loaded with Xyloglucans polysaccharides from Tamarindus flower extract.
33626372	9	31	theme	spherical	1379:1387	arg1	shape					1389:1393	spherical shape	1379:1393	spherical shape	1379:1393	EDX analysis exhibited the presence of O, Na, P and Cl, while DLS confirmed bilayer formation of xyloglucan and liposomes with uniform size (70-80 nm) and spherical shape.
33626372	5	32	theme	biological	672:681	arg1	molecules					683:691	The biological molecules	668:691	The biological molecules of Tamarindus indica flower	668:719	The biological molecules of Tamarindus indica flower were used for the synthesis of nanoliposome.
33626372	2	33	from	rich	257:260	arg1	Xyloglucan					265:274	Xyloglucan	265:274	Xyloglucan	265:274	Tamarind flower extract is rich in Xyloglucan, a starch like polysaccharide which promotes proliferation and various application areas like drug-delivery technology.
33626372	2	33	from	rich	257:260	arg1	starch					279:284	a starch	277:284	a starch like polysaccharide which promotes proliferation and various application areas like drug-delivery technology	277:393	Tamarind flower extract is rich in Xyloglucan, a starch like polysaccharide which promotes proliferation and various application areas like drug-delivery technology.
33626372	3	34	dep	in	458:459	arg1	vitro					461:465	vitro	461:465	vitro	461:465	In recent years researchers are evaluating nanoliposome using in vitro and in vivo studies to discover their biomedical applications.
33626372	6	35	theme	Tamarindus	782:791	arg1	TifeLiNPs					836:844	TifeLiNPs	836:844	TifeLiNPs	836:844	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	6	35	theme	Tamarindus	782:791	arg1	nanoparticles					821:833	The synthesized Tamarindus indica flower extract lipid nanoparticles	766:833	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans	766:869	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	6	36	dep	in	1027:1028	arg1	vitro					1030:1034	vitro	1030:1034	vitro	1030:1034	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	7	37	theme	UV-Vis	1047:1052	arg1	analysis					1063:1070	UV-Vis spectral analysis	1047:1070	UV-Vis spectral analysis	1047:1070	UV-Vis spectral analysis revealed the emission of peak at 232 nm.
33626372	14	38	theme	present	2161:2167	arg1	study					2169:2173	the present study	2157:2173	the present study	2157:2173	Overall, the present study exhibited the potential application of TifeLiNPs for biomedical purposes.
33626372	2	39	from	Xyloglucan	265:274	arg1	rich					257:260	rich	257:260	rich	257:260	Tamarind flower extract is rich in Xyloglucan, a starch like polysaccharide which promotes proliferation and various application areas like drug-delivery technology.
33626372	0	40	theme	In	0:1	arg1	evaluation					21:30	In vitro therapeutic evaluation	0:30	In vitro therapeutic evaluation of nanoliposome	0:46	In vitro therapeutic evaluation of nanoliposome loaded with Xyloglucans polysaccharides from Tamarindus flower extract.
33626372	11	41	theme	carbohydrate	1631:1642	arg1	polymers					1644:1651	carbohydrate polymers	1631:1651	carbohydrate polymers such as rhamnose, arabinose, galactose, glucose and xylose revealed using high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system	1631:1835	Presence of carbohydrate polymers such as rhamnose, arabinose, galactose, glucose and xylose revealed using high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system were measured using Pulsed Amperometric Detection (PAD).
33626372	11	41	theme	carbohydrate	1631:1642	arg1	rhamnose					1661:1668	rhamnose	1661:1668	rhamnose	1661:1668	Presence of carbohydrate polymers such as rhamnose, arabinose, galactose, glucose and xylose revealed using high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system were measured using Pulsed Amperometric Detection (PAD).
33626372	8	42	theme	components	1188:1197	arg1	presence					1176:1183	the presence	1172:1183	the presence of components in the functional group	1172:1221	Further, the chemical characterization using FTIR revealed the presence of components in the functional group.
33626372	14	43	theme	biomedical	2228:2237	arg1	purposes					2239:2246	biomedical purposes	2228:2246	biomedical purposes	2228:2246	Overall, the present study exhibited the potential application of TifeLiNPs for biomedical purposes.
33626372	12	44	theme	potential	1989:1997	arg1	activity					2013:2020	potential antibacterial activity	1989:2020	potential antibacterial activity	1989:2020	The synthesized nanoliposome evaluated against Gram negative and Gram positive bacteria showed potential antibacterial activity.
33626372	3	45	theme	in	458:459	arg1	studies					479:485	in vitro and in vivo studies	458:485	in vitro and in vivo studies	458:485	In recent years researchers are evaluating nanoliposome using in vitro and in vivo studies to discover their biomedical applications.
33626372	14	46	theme	TifeLiNPs	2214:2222	arg1	application					2199:2209	the potential application	2185:2209	the potential application of TifeLiNPs for biomedical purposes	2185:2246	Overall, the present study exhibited the potential application of TifeLiNPs for biomedical purposes.
33626372	11	47	theme	basic	1787:1791	arg1	conditions					1793:1802	basic conditions	1787:1802	basic conditions	1787:1802	Presence of carbohydrate polymers such as rhamnose, arabinose, galactose, glucose and xylose revealed using high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system were measured using Pulsed Amperometric Detection (PAD).
33626372	12	48	theme	negative	1946:1953	arg1	bacteria					1973:1980	Gram negative and Gram positive bacteria	1941:1980	Gram negative and Gram positive bacteria	1941:1980	The synthesized nanoliposome evaluated against Gram negative and Gram positive bacteria showed potential antibacterial activity.
33626372	2	49	theme	flower	239:244	arg1	extract					246:252	Tamarind flower extract	230:252	Tamarind flower extract	230:252	Tamarind flower extract is rich in Xyloglucan, a starch like polysaccharide which promotes proliferation and various application areas like drug-delivery technology.
33626372	10	50	theme	Physicochemical	1400:1414	arg1	characterization					1416:1431	The Physicochemical characterization	1396:1431	The Physicochemical characterization of tamarind flower extract for its chemical composition	1396:1487	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	2	51	theme	drug-delivery	370:382	arg1	technology					384:393	drug-delivery technology	370:393	drug-delivery technology	370:393	Tamarind flower extract is rich in Xyloglucan, a starch like polysaccharide which promotes proliferation and various application areas like drug-delivery technology.
33626372	6	52	theme	extract	807:813	arg1	TifeLiNPs					836:844	TifeLiNPs	836:844	TifeLiNPs	836:844	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	6	52	theme	extract	807:813	arg1	nanoparticles					821:833	The synthesized Tamarindus indica flower extract lipid nanoparticles	766:833	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans	766:869	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	11	53	theme	ion	1810:1812	arg1	system					1830:1835	an ion chromatographic system	1807:1835	an ion chromatographic system	1807:1835	Presence of carbohydrate polymers such as rhamnose, arabinose, galactose, glucose and xylose revealed using high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system were measured using Pulsed Amperometric Detection (PAD).
33626372	6	54	theme	lipid	815:819	arg1	TifeLiNPs					836:844	TifeLiNPs	836:844	TifeLiNPs	836:844	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	6	54	theme	lipid	815:819	arg1	nanoparticles					821:833	The synthesized Tamarindus indica flower extract lipid nanoparticles	766:833	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans	766:869	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	0	55	dep	In	0:1	arg1	therapeutic					9:19	therapeutic	9:19	therapeutic	9:19	In vitro therapeutic evaluation of nanoliposome loaded with Xyloglucans polysaccharides from Tamarindus flower extract.
33626372	0	55	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro therapeutic evaluation of nanoliposome loaded with Xyloglucans polysaccharides from Tamarindus flower extract.
33626372	12	56	theme	synthesized	1898:1908	arg1	nanoliposome					1910:1921	The synthesized nanoliposome	1894:1921	The synthesized nanoliposome evaluated against Gram negative and Gram positive bacteria	1894:1980	The synthesized nanoliposome evaluated against Gram negative and Gram positive bacteria showed potential antibacterial activity.
33626372	10	57	from	presence	1502:1509	arg1	test					1613:1616	confirmatory test	1600:1616	confirmatory test	1600:1616	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	11	58	theme	exchange	1750:1757	arg1	chromatography					1766:1779	high performance anion exchange (HPAE) chromatography	1727:1779	high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system	1727:1835	Presence of carbohydrate polymers such as rhamnose, arabinose, galactose, glucose and xylose revealed using high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system were measured using Pulsed Amperometric Detection (PAD).
33626372	6	59	theme	flower	800:805	arg1	TifeLiNPs					836:844	TifeLiNPs	836:844	TifeLiNPs	836:844	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	6	59	theme	flower	800:805	arg1	nanoparticles					821:833	The synthesized Tamarindus indica flower extract lipid nanoparticles	766:833	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans	766:869	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	4	60	theme	liposomes	635:643	arg1	synthesis					622:630	synthesis	622:630	synthesis of liposomes via biological method	622:665	Considering the importance and feasibility of nanoliposome, the present study is focused on synthesis of liposomes via biological method.
33626372	11	61	theme	performance	1732:1742	arg1	chromatography					1766:1779	high performance anion exchange (HPAE) chromatography	1727:1779	high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system	1727:1835	Presence of carbohydrate polymers such as rhamnose, arabinose, galactose, glucose and xylose revealed using high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system were measured using Pulsed Amperometric Detection (PAD).
33626372	13	62	theme	anti-cancer	2104:2114	arg1	activity					2138:2145	significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity	2046:2145	significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity	2046:2145	TifeLiNPs demonstrated significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity.
33626372	4	63	theme	biological	649:658	arg1	method					660:665	biological method	649:665	biological method	649:665	Considering the importance and feasibility of nanoliposome, the present study is focused on synthesis of liposomes via biological method.
33626372	10	64	theme	chemical	1468:1475	arg1	composition					1477:1487	its chemical composition	1464:1487	its chemical composition	1464:1487	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	1	65	theme	diagnostic	189:198	arg1	applications					216:227	several diagnostic and therapeutic applications	181:227	several diagnostic and therapeutic applications	181:227	Nanoparticles are interesting area of research developed for several diagnostic and therapeutic applications.
33626372	11	66	theme	HPAE	1760:1763	arg1	chromatography					1766:1779	high performance anion exchange (HPAE) chromatography	1727:1779	high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system	1727:1835	Presence of carbohydrate polymers such as rhamnose, arabinose, galactose, glucose and xylose revealed using high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system were measured using Pulsed Amperometric Detection (PAD).
33626372	5	67	theme	indica	707:712	arg1	flower					714:719	Tamarindus indica flower	696:719	Tamarindus indica flower	696:719	The biological molecules of Tamarindus indica flower were used for the synthesis of nanoliposome.
33626372	10	68	theme	carbohydrates	1514:1526	arg1	presence					1502:1509	the presence	1498:1509	the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test	1498:1616	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	1	69	theme	therapeutic	204:214	arg1	applications					216:227	several diagnostic and therapeutic applications	181:227	several diagnostic and therapeutic applications	181:227	Nanoparticles are interesting area of research developed for several diagnostic and therapeutic applications.
33626372	5	70	theme	flower	714:719	arg1	molecules					683:691	The biological molecules	668:691	The biological molecules of Tamarindus indica flower	668:719	The biological molecules of Tamarindus indica flower were used for the synthesis of nanoliposome.
33626372	7	71	from	232 nm	1105:1110	arg1	emission					1085:1092	the emission	1081:1092	the emission of peak at 232 nm	1081:1110	UV-Vis spectral analysis revealed the emission of peak at 232 nm.
33626372	9	72	theme	bilayer	1300:1306	arg1	formation					1308:1316	bilayer formation	1300:1316	bilayer formation of xyloglucan and liposomes	1300:1344	EDX analysis exhibited the presence of O, Na, P and Cl, while DLS confirmed bilayer formation of xyloglucan and liposomes with uniform size (70-80 nm) and spherical shape.
33626372	2	73	theme	various	339:345	arg1	areas					359:363	various application areas	339:363	various application areas	339:363	Tamarind flower extract is rich in Xyloglucan, a starch like polysaccharide which promotes proliferation and various application areas like drug-delivery technology.
33626372	13	74	theme	significant	2046:2056	arg1	activity					2138:2145	significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity	2046:2145	significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity	2046:2145	TifeLiNPs demonstrated significant in vitro antioxidant potential, antidiabetic, anti-cancer and anti-inflammatory activity.
33626372	5	75	theme	nanoliposome	752:763	arg1	synthesis					739:747	the synthesis	735:747	the synthesis of nanoliposome	735:763	The biological molecules of Tamarindus indica flower were used for the synthesis of nanoliposome.
33626372	0	76	from	extract	111:117	arg1	polysaccharides					72:86	Xyloglucans polysaccharides	60:86	Xyloglucans polysaccharides from Tamarindus flower extract	60:117	In vitro therapeutic evaluation of nanoliposome loaded with Xyloglucans polysaccharides from Tamarindus flower extract.
33626372	11	77	theme	Pulsed	1857:1862	arg1	Detection					1877:1885	Pulsed Amperometric Detection	1857:1885	Pulsed Amperometric Detection (PAD)	1857:1891	Presence of carbohydrate polymers such as rhamnose, arabinose, galactose, glucose and xylose revealed using high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system were measured using Pulsed Amperometric Detection (PAD).
33626372	11	77	theme	Pulsed	1857:1862	arg1	PAD					1888:1890	PAD	1888:1890	PAD	1888:1890	Presence of carbohydrate polymers such as rhamnose, arabinose, galactose, glucose and xylose revealed using high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system were measured using Pulsed Amperometric Detection (PAD).
33626372	1	78	theme	research	158:165	arg1	area					150:153	interesting area	138:153	interesting area of research developed for several diagnostic and therapeutic applications	138:227	Nanoparticles are interesting area of research developed for several diagnostic and therapeutic applications.
33626372	1	78	theme	research	158:165	arg1	Nanoparticles					120:132	Nanoparticles	120:132	Nanoparticles	120:132	Nanoparticles are interesting area of research developed for several diagnostic and therapeutic applications.
33626372	0	79	theme	Tamarindus	93:102	arg1	extract					111:117	Tamarindus flower extract	93:117	Tamarindus flower extract	93:117	In vitro therapeutic evaluation of nanoliposome loaded with Xyloglucans polysaccharides from Tamarindus flower extract.
33626372	7	80	theme	peak	1097:1100	arg1	emission					1085:1092	the emission	1081:1092	the emission of peak at 232 nm	1081:1110	UV-Vis spectral analysis revealed the emission of peak at 232 nm.
33626372	10	81	theme	terpenoids	1540:1549	arg1	presence					1502:1509	the presence	1498:1509	the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test	1498:1616	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	10	82	theme	alkaloids	1529:1537	arg1	presence					1502:1509	the presence	1498:1509	the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test	1498:1616	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	6	83	theme	indica	793:798	arg1	TifeLiNPs					836:844	TifeLiNPs	836:844	TifeLiNPs	836:844	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	6	83	theme	indica	793:798	arg1	nanoparticles					821:833	The synthesized Tamarindus indica flower extract lipid nanoparticles	766:833	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans	766:869	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	14	84	theme	potential	2189:2197	arg1	application					2199:2209	the potential application	2185:2209	the potential application of TifeLiNPs for biomedical purposes	2185:2246	Overall, the present study exhibited the potential application of TifeLiNPs for biomedical purposes.
33626372	3	85	theme	recent	399:404	arg1	years					406:410	recent years	399:410	recent years	399:410	In recent years researchers are evaluating nanoliposome using in vitro and in vivo studies to discover their biomedical applications.
33626372	10	86	theme	saponins	1564:1571	arg1	presence					1502:1509	the presence	1498:1509	the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test	1498:1616	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	6	87	theme	synthesized	770:780	arg1	TifeLiNPs					836:844	TifeLiNPs	836:844	TifeLiNPs	836:844	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	6	87	theme	synthesized	770:780	arg1	nanoparticles					821:833	The synthesized Tamarindus indica flower extract lipid nanoparticles	766:833	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans	766:869	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	7	88	theme	spectral	1054:1061	arg1	analysis					1063:1070	UV-Vis spectral analysis	1047:1070	UV-Vis spectral analysis	1047:1070	UV-Vis spectral analysis revealed the emission of peak at 232 nm.
33626372	11	89	theme	polymers	1644:1651	arg1	Presence					1619:1626	Presence	1619:1626	Presence of carbohydrate polymers such as rhamnose, arabinose, galactose, glucose and xylose revealed using high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system	1619:1835	Presence of carbohydrate polymers such as rhamnose, arabinose, galactose, glucose and xylose revealed using high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system were measured using Pulsed Amperometric Detection (PAD).
33626372	3	90	dep	in	471:472	arg1	vivo					474:477	vivo	474:477	vivo	474:477	In recent years researchers are evaluating nanoliposome using in vitro and in vivo studies to discover their biomedical applications.
33626372	4	91	dep	importance	546:555	arg1	the					542:544	the	542:544	the	542:544	Considering the importance and feasibility of nanoliposome, the present study is focused on synthesis of liposomes via biological method.
33626372	11	92	from	chromatography	1766:1779	arg1	system					1830:1835	an ion chromatographic system	1807:1835	an ion chromatographic system	1807:1835	Presence of carbohydrate polymers such as rhamnose, arabinose, galactose, glucose and xylose revealed using high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system were measured using Pulsed Amperometric Detection (PAD).
33626372	10	93	theme	flavonoids	1586:1595	arg1	presence					1502:1509	the presence	1498:1509	the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test	1498:1616	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	9	94	theme	EDX	1224:1226	arg1	analysis					1228:1235	EDX analysis	1224:1235	EDX analysis	1224:1235	EDX analysis exhibited the presence of O, Na, P and Cl, while DLS confirmed bilayer formation of xyloglucan and liposomes with uniform size (70-80 nm) and spherical shape.
33626372	0	95	theme	nanoliposome	35:46	arg1	evaluation					21:30	In vitro therapeutic evaluation	0:30	In vitro therapeutic evaluation of nanoliposome	0:46	In vitro therapeutic evaluation of nanoliposome loaded with Xyloglucans polysaccharides from Tamarindus flower extract.
33626372	8	96	theme	functional	1206:1215	arg1	group					1217:1221	the functional group	1202:1221	the functional group	1202:1221	Further, the chemical characterization using FTIR revealed the presence of components in the functional group.
33626372	6	97	theme	antidiabetic	962:973	arg1	applications					920:931	therapeutic applications	908:931	therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities)	908:1019	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	6	97	theme	antidiabetic	962:973	arg1	activities					1009:1018	antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities	934:1018	antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities	934:1018	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	8	98	attach	presence	1176:1183	arg2	components					1188:1197	components	1188:1197	components	1188:1197	Further, the chemical characterization using FTIR revealed the presence of components in the functional group.
33626372	8	98	attach	presence	1176:1183	arg1	group					1217:1221	the functional group	1202:1221	the functional group	1202:1221	Further, the chemical characterization using FTIR revealed the presence of components in the functional group.
33626372	3	99	theme	in	471:472	arg1	studies					479:485	in vitro and in vivo studies	458:485	in vitro and in vivo studies	458:485	In recent years researchers are evaluating nanoliposome using in vitro and in vivo studies to discover their biomedical applications.
33626372	4	100	theme	present	594:600	arg1	study					602:606	the present study	590:606	the present study	590:606	Considering the importance and feasibility of nanoliposome, the present study is focused on synthesis of liposomes via biological method.
33626372	10	101	theme	tannins	1574:1580	arg1	presence					1502:1509	the presence	1498:1509	the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test	1498:1616	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	10	102	theme	tamarind	1436:1443	arg1	extract					1452:1458	tamarind flower extract	1436:1458	tamarind flower extract	1436:1458	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	3	103	theme	biomedical	505:514	arg1	applications					516:527	their biomedical applications	499:527	their biomedical applications	499:527	In recent years researchers are evaluating nanoliposome using in vitro and in vivo studies to discover their biomedical applications.
33626372	6	104	theme	antioxidant	949:959	arg1	applications					920:931	therapeutic applications	908:931	therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities)	908:1019	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	6	104	theme	antioxidant	949:959	arg1	activities					1009:1018	antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities	934:1018	antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities	934:1018	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	11	105	theme	chromatographic	1814:1828	arg1	system					1830:1835	an ion chromatographic system	1807:1835	an ion chromatographic system	1807:1835	Presence of carbohydrate polymers such as rhamnose, arabinose, galactose, glucose and xylose revealed using high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system were measured using Pulsed Amperometric Detection (PAD).
33626372	12	106	theme	positive	1964:1971	arg1	bacteria					1973:1980	Gram negative and Gram positive bacteria	1941:1980	Gram negative and Gram positive bacteria	1941:1980	The synthesized nanoliposome evaluated against Gram negative and Gram positive bacteria showed potential antibacterial activity.
33626372	6	107	theme	antibacterial	934:946	arg1	applications					920:931	therapeutic applications	908:931	therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities)	908:1019	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	6	107	theme	antibacterial	934:946	arg1	activities					1009:1018	antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities	934:1018	antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities	934:1018	The synthesized Tamarindus indica flower extract lipid nanoparticles (TifeLiNPs) loaded with xyloglucans were characterized and evaluated for therapeutic applications (antibacterial, antioxidant, antidiabetic, anticancer and anti-inflammatory activities) under in vitro condition.
33626372	9	108	theme	Na	1266:1267	arg1	presence					1251:1258	the presence	1247:1258	the presence of O, Na, P and Cl	1247:1277	EDX analysis exhibited the presence of O, Na, P and Cl, while DLS confirmed bilayer formation of xyloglucan and liposomes with uniform size (70-80 nm) and spherical shape.
33626372	10	109	theme	confirmatory	1600:1611	arg1	test					1613:1616	confirmatory test	1600:1616	confirmatory test	1600:1616	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
33626372	2	110	theme	Tamarind	230:237	arg1	extract					246:252	Tamarind flower extract	230:252	Tamarind flower extract	230:252	Tamarind flower extract is rich in Xyloglucan, a starch like polysaccharide which promotes proliferation and various application areas like drug-delivery technology.
33626372	9	111	theme	O	1263:1263	arg1	presence					1251:1258	the presence	1247:1258	the presence of O, Na, P and Cl	1247:1277	EDX analysis exhibited the presence of O, Na, P and Cl, while DLS confirmed bilayer formation of xyloglucan and liposomes with uniform size (70-80 nm) and spherical shape.
33626372	11	112	theme	anion	1744:1748	arg1	chromatography					1766:1779	high performance anion exchange (HPAE) chromatography	1727:1779	high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system	1727:1835	Presence of carbohydrate polymers such as rhamnose, arabinose, galactose, glucose and xylose revealed using high performance anion exchange (HPAE) chromatography under basic conditions on an ion chromatographic system were measured using Pulsed Amperometric Detection (PAD).
33626372	10	113	theme	extract	1452:1458	arg1	characterization					1416:1431	The Physicochemical characterization	1396:1431	The Physicochemical characterization of tamarind flower extract for its chemical composition	1396:1487	The Physicochemical characterization of tamarind flower extract for its chemical composition revealed the presence of carbohydrates, alkaloids, terpenoids, glycosides, saponins, tannins and flavonoids in confirmatory test.
34743027	4	0	from	sequence	684:691	arg1	MWCO					658:661	MWCO	658:661	MWCO of 300 and 10 kDa in sequence	658:691	Results indicated that three polysaccharide fractions named as QPs-I, QPs-II and QPs-III were successfully separated using microfiltration and ultrafiltration membrane with MWCO of 300 and 10 kDa in sequence.
34743027	5	1	dep	4609 Da	759:765	arg1	%					773:773	33.75%	768:773	33.75%	768:773	The Mw and polysaccharide content of three fractions were QPs-I (4609 Da, 33.75%), QPs-II (15,932 Da, 45.31%) and QPs-III (960,895 Da, 34.65%), respectively.
34743027	0	2	from	properties	16:25	arg1	quinoa					106:111	quinoa	106:111	quinoa	106:111	Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.)
34743027	5	3	dep	15,932 Da	785:793	arg1	%					801:801	45.31%	796:801	45.31%	796:801	The Mw and polysaccharide content of three fractions were QPs-I (4609 Da, 33.75%), QPs-II (15,932 Da, 45.31%) and QPs-III (960,895 Da, 34.65%), respectively.
34743027	8	4	theme	immunoregulatory	1285:1300	arg1	activity					1302:1309	immunoregulatory activity	1285:1309	immunoregulatory activity on RAW264.7 cells	1285:1327	Biological experiment showed that antioxidant and antidiabetic activities in dose-dependent manners and also revealed immunoregulatory activity on RAW264.7 cells.
34743027	0	5	theme	Chenopodium	114:124	arg1	quinoa					126:131	Chenopodium quinoa	114:131	Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.)	0:139	Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.)
34743027	9	6	contain	has	1363:1365	arg1	QPs					1359:1361	QPs	1359:1361	QPs	1359:1361	These results indicated that QPs has the potential to be used in a natural agent in antioxidant, antidiabetic and immunoregulation functional food.
34743027	9	6	contain	has	1363:1365	arg2	potential					1371:1379	the potential to be used in a natural agent in antioxidant, antidiabetic and immunoregulation functional food	1367:1475	the potential to be used in a natural agent in antioxidant, antidiabetic and immunoregulation functional food	1367:1475	These results indicated that QPs has the potential to be used in a natural agent in antioxidant, antidiabetic and immunoregulation functional food.
34743027	8	7	theme	RAW264.7	1314:1321	arg1	cells					1323:1327	RAW264.7 cells	1314:1327	RAW264.7 cells	1314:1327	Biological experiment showed that antioxidant and antidiabetic activities in dose-dependent manners and also revealed immunoregulatory activity on RAW264.7 cells.
34743027	3	8	theme	instrumental	388:399	arg1	analysis					401:408	instrumental analysis	388:408	instrumental analysis	388:408	The chemical composition, structure characteristic and morphology were analyzed by chemical methods and instrumental analysis including HPLC-DAD, UV, FT-IR, Congo red test, SEM, AFM, XRD, TGA and NMR.
34743027	0	9	from	activities	71:80	arg1	quinoa					106:111	quinoa	106:111	quinoa	106:111	Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.)
34743027	6	10	from	%	1031:1031	arg1	QPs-II					1053:1058	QPs-II	1053:1058	QPs-II	1053:1058	The polysaccharide in three fractions was heteropolysaccharide that mainly consisted of glucose, galactose and arabinose, with their combined monosaccharide percentage being 91.17% in QPs-I, 87.81% in QPs-II, and 91.72% in QPs-III, respectively.
34743027	6	10	from	%	1031:1031	arg1	QPs-I					1036:1040	QPs-I	1036:1040	QPs-I	1036:1040	The polysaccharide in three fractions was heteropolysaccharide that mainly consisted of glucose, galactose and arabinose, with their combined monosaccharide percentage being 91.17% in QPs-I, 87.81% in QPs-II, and 91.72% in QPs-III, respectively.
34743027	6	10	from	%	1031:1031	arg1	QPs-III					1075:1081	QPs-III	1075:1081	QPs-III	1075:1081	The polysaccharide in three fractions was heteropolysaccharide that mainly consisted of glucose, galactose and arabinose, with their combined monosaccharide percentage being 91.17% in QPs-I, 87.81% in QPs-II, and 91.72% in QPs-III, respectively.
34743027	9	11	theme	antioxidant	1414:1424	arg1	food					1472:1475	antioxidant, antidiabetic and immunoregulation functional food	1414:1475	antioxidant, antidiabetic and immunoregulation functional food	1414:1475	These results indicated that QPs has the potential to be used in a natural agent in antioxidant, antidiabetic and immunoregulation functional food.
34743027	3	12	theme	chemical	367:374	arg1	HPLC-DAD					420:427	HPLC-DAD	420:427	HPLC-DAD	420:427	The chemical composition, structure characteristic and morphology were analyzed by chemical methods and instrumental analysis including HPLC-DAD, UV, FT-IR, Congo red test, SEM, AFM, XRD, TGA and NMR.
34743027	3	12	theme	chemical	367:374	arg1	test					451:454	Congo red test	441:454	Congo red test	441:454	The chemical composition, structure characteristic and morphology were analyzed by chemical methods and instrumental analysis including HPLC-DAD, UV, FT-IR, Congo red test, SEM, AFM, XRD, TGA and NMR.
34743027	3	12	theme	chemical	367:374	arg1	methods					376:382	chemical methods	367:382	chemical methods	367:382	The chemical composition, structure characteristic and morphology were analyzed by chemical methods and instrumental analysis including HPLC-DAD, UV, FT-IR, Congo red test, SEM, AFM, XRD, TGA and NMR.
34743027	3	12	theme	chemical	367:374	arg1	XRD					467:469	XRD	467:469	XRD	467:469	The chemical composition, structure characteristic and morphology were analyzed by chemical methods and instrumental analysis including HPLC-DAD, UV, FT-IR, Congo red test, SEM, AFM, XRD, TGA and NMR.
34743027	3	12	theme	chemical	367:374	arg1	UV					430:431	UV	430:431	UV	430:431	The chemical composition, structure characteristic and morphology were analyzed by chemical methods and instrumental analysis including HPLC-DAD, UV, FT-IR, Congo red test, SEM, AFM, XRD, TGA and NMR.
34743027	3	12	theme	chemical	367:374	arg1	TGA					472:474	TGA	472:474	TGA	472:474	The chemical composition, structure characteristic and morphology were analyzed by chemical methods and instrumental analysis including HPLC-DAD, UV, FT-IR, Congo red test, SEM, AFM, XRD, TGA and NMR.
34743027	3	12	theme	chemical	367:374	arg1	FT-IR					434:438	FT-IR	434:438	FT-IR	434:438	The chemical composition, structure characteristic and morphology were analyzed by chemical methods and instrumental analysis including HPLC-DAD, UV, FT-IR, Congo red test, SEM, AFM, XRD, TGA and NMR.
34743027	3	12	theme	chemical	367:374	arg1	SEM					457:459	SEM	457:459	SEM	457:459	The chemical composition, structure characteristic and morphology were analyzed by chemical methods and instrumental analysis including HPLC-DAD, UV, FT-IR, Congo red test, SEM, AFM, XRD, TGA and NMR.
34743027	3	12	theme	chemical	367:374	arg1	AFM					462:464	AFM	462:464	AFM	462:464	The chemical composition, structure characteristic and morphology were analyzed by chemical methods and instrumental analysis including HPLC-DAD, UV, FT-IR, Congo red test, SEM, AFM, XRD, TGA and NMR.
34743027	3	12	theme	chemical	367:374	arg1	NMR					480:482	NMR	480:482	NMR	480:482	The chemical composition, structure characteristic and morphology were analyzed by chemical methods and instrumental analysis including HPLC-DAD, UV, FT-IR, Congo red test, SEM, AFM, XRD, TGA and NMR.
34743027	8	13	theme	antioxidant	1201:1211	arg1	activities					1230:1239	that antioxidant and antidiabetic activities	1196:1239	that antioxidant and antidiabetic activities in dose-dependent manners	1196:1265	Biological experiment showed that antioxidant and antidiabetic activities in dose-dependent manners and also revealed immunoregulatory activity on RAW264.7 cells.
34743027	4	14	from	300	666:668	arg1	sequence					684:691	sequence	684:691	sequence	684:691	Results indicated that three polysaccharide fractions named as QPs-I, QPs-II and QPs-III were successfully separated using microfiltration and ultrafiltration membrane with MWCO of 300 and 10 kDa in sequence.
34743027	5	15	theme	fractions	737:745	arg1	QPs-I					752:756	QPs-I	752:756	QPs-I	752:756	The Mw and polysaccharide content of three fractions were QPs-I (4609 Da, 33.75%), QPs-II (15,932 Da, 45.31%) and QPs-III (960,895 Da, 34.65%), respectively.
34743027	5	15	theme	fractions	737:745	arg1	content					720:726	The Mw and polysaccharide content	694:726	content	720:726	The Mw and polysaccharide content of three fractions were QPs-I (4609 Da, 33.75%), QPs-II (15,932 Da, 45.31%) and QPs-III (960,895 Da, 34.65%), respectively.
34743027	8	16	from	activity	1302:1309	arg1	cells					1323:1327	RAW264.7 cells	1314:1327	RAW264.7 cells	1314:1327	Biological experiment showed that antioxidant and antidiabetic activities in dose-dependent manners and also revealed immunoregulatory activity on RAW264.7 cells.
34743027	8	17	theme	Biological	1167:1176	arg1	experiment					1178:1187	Biological experiment	1167:1187	Biological experiment	1167:1187	Biological experiment showed that antioxidant and antidiabetic activities in dose-dependent manners and also revealed immunoregulatory activity on RAW264.7 cells.
34743027	0	18	from	characterization	39:54	arg1	quinoa					106:111	quinoa	106:111	quinoa	106:111	Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.)
34743027	7	19	theme	triple-helix	1143:1154	arg1	structure					1156:1164	triple-helix structure	1143:1164	triple-helix structure	1143:1164	All three polysaccharide fractions contained triple-helix structure.
34743027	4	20	from	MWCO	658:661	arg1	sequence					684:691	sequence	684:691	sequence	684:691	Results indicated that three polysaccharide fractions named as QPs-I, QPs-II and QPs-III were successfully separated using microfiltration and ultrafiltration membrane with MWCO of 300 and 10 kDa in sequence.
34743027	3	21	theme	Congo	441:445	arg1	test					451:454	Congo red test	441:454	Congo red test	441:454	The chemical composition, structure characteristic and morphology were analyzed by chemical methods and instrumental analysis including HPLC-DAD, UV, FT-IR, Congo red test, SEM, AFM, XRD, TGA and NMR.
34743027	0	22	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.)
34743027	3	23	theme	red	447:449	arg1	test					451:454	Congo red test	441:454	Congo red test	441:454	The chemical composition, structure characteristic and morphology were analyzed by chemical methods and instrumental analysis including HPLC-DAD, UV, FT-IR, Congo red test, SEM, AFM, XRD, TGA and NMR.
34743027	0	24	theme	structural	28:37	arg1	characterization					39:54	structural characterization	28:54	structural characterization	28:54	Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.)
34743027	3	25	theme	chemical	288:295	arg1	composition					297:307	The chemical composition	284:307	The chemical composition	284:307	The chemical composition, structure characteristic and morphology were analyzed by chemical methods and instrumental analysis including HPLC-DAD, UV, FT-IR, Congo red test, SEM, AFM, XRD, TGA and NMR.
34743027	2	26	from	extract	259:265	arg1	fractions					234:242	separate polysaccharide fractions	210:242	separate polysaccharide fractions from the water extract of quinoa seeds	210:281	In this paper, membrane separation technology was employed to separate polysaccharide fractions from the water extract of quinoa seeds.
34743027	4	27	theme	ultrafiltration	628:642	arg1	membrane					644:651	ultrafiltration membrane	628:651	ultrafiltration membrane	628:651	Results indicated that three polysaccharide fractions named as QPs-I, QPs-II and QPs-III were successfully separated using microfiltration and ultrafiltration membrane with MWCO of 300 and 10 kDa in sequence.
34743027	7	28	contain	contained	1133:1141	arg2	structure					1156:1164	triple-helix structure	1143:1164	triple-helix structure	1143:1164	All three polysaccharide fractions contained triple-helix structure.
34743027	7	28	contain	contained	1133:1141	arg1	fractions					1123:1131	All three polysaccharide fractions	1098:1131	All three polysaccharide fractions	1098:1131	All three polysaccharide fractions contained triple-helix structure.
34743027	5	29	theme	Mw	698:699	arg1	QPs-I					752:756	QPs-I	752:756	QPs-I	752:756	The Mw and polysaccharide content of three fractions were QPs-I (4609 Da, 33.75%), QPs-II (15,932 Da, 45.31%) and QPs-III (960,895 Da, 34.65%), respectively.
34743027	5	29	theme	Mw	698:699	arg1	content					720:726	The Mw and polysaccharide content	694:726	content	720:726	The Mw and polysaccharide content of three fractions were QPs-I (4609 Da, 33.75%), QPs-II (15,932 Da, 45.31%) and QPs-III (960,895 Da, 34.65%), respectively.
34743027	0	30	from	quinoa	106:111	arg1	activities					71:80	biological activities	60:80	biological activities	60:80	Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.)
34743027	0	30	from	quinoa	106:111	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.)
34743027	0	30	from	quinoa	106:111	arg1	characterization					39:54	structural characterization	28:54	structural characterization	28:54	Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.)
34743027	0	30	from	quinoa	106:111	arg1	polysaccharides					85:99	polysaccharides	85:99	polysaccharides from quinoa	85:111	Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.)
34743027	6	31	from	%	1070:1070	arg1	QPs-II					1053:1058	QPs-II	1053:1058	QPs-II	1053:1058	The polysaccharide in three fractions was heteropolysaccharide that mainly consisted of glucose, galactose and arabinose, with their combined monosaccharide percentage being 91.17% in QPs-I, 87.81% in QPs-II, and 91.72% in QPs-III, respectively.
34743027	6	31	from	%	1070:1070	arg1	QPs-I					1036:1040	QPs-I	1036:1040	QPs-I	1036:1040	The polysaccharide in three fractions was heteropolysaccharide that mainly consisted of glucose, galactose and arabinose, with their combined monosaccharide percentage being 91.17% in QPs-I, 87.81% in QPs-II, and 91.72% in QPs-III, respectively.
34743027	6	31	from	%	1070:1070	arg1	QPs-III					1075:1081	QPs-III	1075:1081	QPs-III	1075:1081	The polysaccharide in three fractions was heteropolysaccharide that mainly consisted of glucose, galactose and arabinose, with their combined monosaccharide percentage being 91.17% in QPs-I, 87.81% in QPs-II, and 91.72% in QPs-III, respectively.
34743027	6	32	theme	combined	985:992	arg1	percentage					1009:1018	their combined monosaccharide percentage	979:1018	their combined monosaccharide percentage	979:1018	The polysaccharide in three fractions was heteropolysaccharide that mainly consisted of glucose, galactose and arabinose, with their combined monosaccharide percentage being 91.17% in QPs-I, 87.81% in QPs-II, and 91.72% in QPs-III, respectively.
34743027	2	33	theme	water	253:257	arg1	extract					259:265	the water extract	249:265	the water extract of quinoa seeds	249:281	In this paper, membrane separation technology was employed to separate polysaccharide fractions from the water extract of quinoa seeds.
34743027	4	34	theme	300	666:668	arg1	MWCO					658:661	MWCO	658:661	MWCO of 300 and 10 kDa in sequence	658:691	Results indicated that three polysaccharide fractions named as QPs-I, QPs-II and QPs-III were successfully separated using microfiltration and ultrafiltration membrane with MWCO of 300 and 10 kDa in sequence.
34743027	9	35	theme	antidiabetic	1427:1438	arg1	food					1472:1475	antioxidant, antidiabetic and immunoregulation functional food	1414:1475	antioxidant, antidiabetic and immunoregulation functional food	1414:1475	These results indicated that QPs has the potential to be used in a natural agent in antioxidant, antidiabetic and immunoregulation functional food.
34743027	5	36	theme	polysaccharide	705:718	arg1	QPs-I					752:756	QPs-I	752:756	QPs-I	752:756	The Mw and polysaccharide content of three fractions were QPs-I (4609 Da, 33.75%), QPs-II (15,932 Da, 45.31%) and QPs-III (960,895 Da, 34.65%), respectively.
34743027	5	36	theme	polysaccharide	705:718	arg1	content					720:726	The Mw and polysaccharide content	694:726	content	720:726	The Mw and polysaccharide content of three fractions were QPs-I (4609 Da, 33.75%), QPs-II (15,932 Da, 45.31%) and QPs-III (960,895 Da, 34.65%), respectively.
34743027	2	37	theme	separation	172:181	arg1	technology					183:192	membrane separation technology	163:192	membrane separation technology	163:192	In this paper, membrane separation technology was employed to separate polysaccharide fractions from the water extract of quinoa seeds.
34743027	5	38	dep	960,895 Da	817:826	arg1	%					834:834	34.65%	829:834	34.65%	829:834	The Mw and polysaccharide content of three fractions were QPs-I (4609 Da, 33.75%), QPs-II (15,932 Da, 45.31%) and QPs-III (960,895 Da, 34.65%), respectively.
34743027	8	39	theme	antidiabetic	1217:1228	arg1	activities					1230:1239	that antioxidant and antidiabetic activities	1196:1239	that antioxidant and antidiabetic activities in dose-dependent manners	1196:1265	Biological experiment showed that antioxidant and antidiabetic activities in dose-dependent manners and also revealed immunoregulatory activity on RAW264.7 cells.
34743027	7	40	theme	polysaccharide	1108:1121	arg1	fractions					1123:1131	All three polysaccharide fractions	1098:1131	All three polysaccharide fractions	1098:1131	All three polysaccharide fractions contained triple-helix structure.
34743027	2	41	theme	membrane	163:170	arg1	technology					183:192	membrane separation technology	163:192	membrane separation technology	163:192	In this paper, membrane separation technology was employed to separate polysaccharide fractions from the water extract of quinoa seeds.
34743027	9	42	from	agent	1405:1409	arg1	food					1472:1475	antioxidant, antidiabetic and immunoregulation functional food	1414:1475	antioxidant, antidiabetic and immunoregulation functional food	1414:1475	These results indicated that QPs has the potential to be used in a natural agent in antioxidant, antidiabetic and immunoregulation functional food.
34743027	6	43	from	%	1048:1048	arg1	QPs-II					1053:1058	QPs-II	1053:1058	QPs-II	1053:1058	The polysaccharide in three fractions was heteropolysaccharide that mainly consisted of glucose, galactose and arabinose, with their combined monosaccharide percentage being 91.17% in QPs-I, 87.81% in QPs-II, and 91.72% in QPs-III, respectively.
34743027	6	43	from	%	1048:1048	arg1	QPs-I					1036:1040	QPs-I	1036:1040	QPs-I	1036:1040	The polysaccharide in three fractions was heteropolysaccharide that mainly consisted of glucose, galactose and arabinose, with their combined monosaccharide percentage being 91.17% in QPs-I, 87.81% in QPs-II, and 91.72% in QPs-III, respectively.
34743027	6	43	from	%	1048:1048	arg1	QPs-III					1075:1081	QPs-III	1075:1081	QPs-III	1075:1081	The polysaccharide in three fractions was heteropolysaccharide that mainly consisted of glucose, galactose and arabinose, with their combined monosaccharide percentage being 91.17% in QPs-I, 87.81% in QPs-II, and 91.72% in QPs-III, respectively.
34743027	8	44	theme	dose-dependent	1244:1257	arg1	manners					1259:1265	dose-dependent manners	1244:1265	dose-dependent manners	1244:1265	Biological experiment showed that antioxidant and antidiabetic activities in dose-dependent manners and also revealed immunoregulatory activity on RAW264.7 cells.
34743027	0	45	theme	biological	60:69	arg1	activities					71:80	biological activities	60:80	biological activities	60:80	Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.)
34743027	4	46	with	microfiltration	608:622	arg1	MWCO					658:661	MWCO	658:661	MWCO of 300 and 10 kDa in sequence	658:691	Results indicated that three polysaccharide fractions named as QPs-I, QPs-II and QPs-III were successfully separated using microfiltration and ultrafiltration membrane with MWCO of 300 and 10 kDa in sequence.
34743027	6	47	from	polysaccharide	856:869	arg1	fractions					880:888	three fractions	874:888	three fractions	874:888	The polysaccharide in three fractions was heteropolysaccharide that mainly consisted of glucose, galactose and arabinose, with their combined monosaccharide percentage being 91.17% in QPs-I, 87.81% in QPs-II, and 91.72% in QPs-III, respectively.
34743027	2	48	theme	polysaccharide	219:232	arg1	fractions					234:242	separate polysaccharide fractions	210:242	separate polysaccharide fractions from the water extract of quinoa seeds	210:281	In this paper, membrane separation technology was employed to separate polysaccharide fractions from the water extract of quinoa seeds.
34743027	4	49	with	membrane	644:651	arg1	MWCO					658:661	MWCO	658:661	MWCO of 300 and 10 kDa in sequence	658:691	Results indicated that three polysaccharide fractions named as QPs-I, QPs-II and QPs-III were successfully separated using microfiltration and ultrafiltration membrane with MWCO of 300 and 10 kDa in sequence.
34743027	9	50	theme	functional	1461:1470	arg1	food					1472:1475	antioxidant, antidiabetic and immunoregulation functional food	1414:1475	antioxidant, antidiabetic and immunoregulation functional food	1414:1475	These results indicated that QPs has the potential to be used in a natural agent in antioxidant, antidiabetic and immunoregulation functional food.
34743027	4	51	theme	polysaccharide	514:527	arg1	fractions					529:537	three polysaccharide fractions	508:537	three polysaccharide fractions named as QPs-I, QPs-II and QPs-III	508:572	Results indicated that three polysaccharide fractions named as QPs-I, QPs-II and QPs-III were successfully separated using microfiltration and ultrafiltration membrane with MWCO of 300 and 10 kDa in sequence.
34743027	2	52	theme	separate	210:217	arg1	fractions					234:242	separate polysaccharide fractions	210:242	separate polysaccharide fractions from the water extract of quinoa seeds	210:281	In this paper, membrane separation technology was employed to separate polysaccharide fractions from the water extract of quinoa seeds.
34743027	2	53	theme	seeds	277:281	arg1	extract					259:265	the water extract	249:265	the water extract of quinoa seeds	249:281	In this paper, membrane separation technology was employed to separate polysaccharide fractions from the water extract of quinoa seeds.
34743027	3	54	theme	structure	310:318	arg1	characteristic					320:333	structure characteristic	310:333	structure characteristic	310:333	The chemical composition, structure characteristic and morphology were analyzed by chemical methods and instrumental analysis including HPLC-DAD, UV, FT-IR, Congo red test, SEM, AFM, XRD, TGA and NMR.
34743027	2	55	theme	quinoa	270:275	arg1	seeds					277:281	quinoa seeds	270:281	quinoa seeds	270:281	In this paper, membrane separation technology was employed to separate polysaccharide fractions from the water extract of quinoa seeds.
34743027	8	56	from	activities	1230:1239	arg1	manners					1259:1265	dose-dependent manners	1244:1265	dose-dependent manners	1244:1265	Biological experiment showed that antioxidant and antidiabetic activities in dose-dependent manners and also revealed immunoregulatory activity on RAW264.7 cells.
34743027	0	57	theme	polysaccharides	85:99	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.)
34743027	0	57	theme	polysaccharides	85:99	arg1	characterization					39:54	structural characterization	28:54	structural characterization	28:54	Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.)
34743027	0	57	theme	polysaccharides	85:99	arg1	activities					71:80	biological activities	60:80	biological activities	60:80	Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.)
34743027	0	58	dep	properties	16:25	arg1	Willd					133:137	Willd	133:137	Willd	133:137	Physicochemical properties, structural characterization and biological activities of polysaccharides from quinoa (Chenopodium quinoa Willd.)
34743027	6	59	theme	monosaccharide	994:1007	arg1	percentage					1009:1018	their combined monosaccharide percentage	979:1018	their combined monosaccharide percentage	979:1018	The polysaccharide in three fractions was heteropolysaccharide that mainly consisted of glucose, galactose and arabinose, with their combined monosaccharide percentage being 91.17% in QPs-I, 87.81% in QPs-II, and 91.72% in QPs-III, respectively.
34743027	9	60	theme	natural	1397:1403	arg1	agent					1405:1409	a natural agent	1395:1409	a natural agent in antioxidant, antidiabetic and immunoregulation functional food	1395:1475	These results indicated that QPs has the potential to be used in a natural agent in antioxidant, antidiabetic and immunoregulation functional food.
34743027	4	61	theme	10 kDa	674:679	arg1	MWCO					658:661	MWCO	658:661	MWCO of 300 and 10 kDa in sequence	658:691	Results indicated that three polysaccharide fractions named as QPs-I, QPs-II and QPs-III were successfully separated using microfiltration and ultrafiltration membrane with MWCO of 300 and 10 kDa in sequence.
33668637	4	0	theme	p	987:987	arg1	<					989:989	p < 0.05	987:994	p < 0.05 in ileum	987:1003	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	1	from	sulfoglycans	1340:1351	arg1	ileum					1360:1364	the ileum	1356:1364	the ileum	1356:1364	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	3	2	theme	studies.The	703:713	arg1	results					715:721	morphological, morphometric, conventional and lectin glycohistochemical studies.The results	631:721	morphological, morphometric, conventional and lectin glycohistochemical studies.The results were analyzed for statistical significance by Student's t test	631:784	Samples from the duodenum, ileum and caecum were collected and processed for morphological, morphometric, conventional and lectin glycohistochemical studies.The results were analyzed for statistical significance by Student's t test.
33668637	1	3	theme	synonymous	251:260	arg1	health					273:278	synonymous withanimal health	251:278	synonymous withanimal health	251:278	Probiotics have become highly recognized as supplements for poultry.Since gut health can be considered synonymous withanimal health, the effects of probiotic Slab51® on the morphology and the glycan composition of guineafowlintestine were examined.
33668637	5	4	theme	fowl	1710:1713	arg1	intestine					1715:1723	guinea fowl intestine	1703:1723	guinea fowl intestine	1703:1723	Overall, dietary Slab51® induces morphological and region-specific changes in glycoprotein composition of guinea fowl intestine, promoting gut health.
33668637	4	5	theme	villus	871:876	arg1	height					878:883	villus height	871:883	villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum)	871:937	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	5	6	from	changes	1664:1670	arg1	composition					1688:1698	glycoprotein composition	1675:1698	glycoprotein composition of guinea fowl intestine	1675:1723	Overall, dietary Slab51® induces morphological and region-specific changes in glycoprotein composition of guinea fowl intestine, promoting gut health.
33668637	4	7	dep	depth	946:950	arg1	<					955:955	p < 0.001	953:961	p < 0.001 in duodenum and caecum	953:984	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	3	8	theme	t	779:779	arg1	test					781:784	Student's t test	769:784	Student's t test	769:784	Samples from the duodenum, ileum and caecum were collected and processed for morphological, morphometric, conventional and lectin glycohistochemical studies.The results were analyzed for statistical significance by Student's t test.
33668637	4	9	from	sulfomucins	1475:1485	arg1	caecum					1494:1499	the caecum	1490:1499	the caecum	1490:1499	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	10	theme	GCs-producing	1461:1473	arg1	sulfomucins					1475:1485	GCs-producing sulfomucins	1461:1485	GCs-producing sulfomucins in the caecum	1461:1499	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	11	theme	cells	1017:1021	arg1	increase					1084:1091	goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase	1010:1091	goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans	1010:1194	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	12	from	<	921:921	arg1	caecum					931:936	caecum	931:936	caecum	931:936	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	1	13	theme	poultry.Since	208:220	arg1	health					226:231	poultry.Since gut health	208:231	poultry.Since gut health	208:231	Probiotics have become highly recognized as supplements for poultry.Since gut health can be considered synonymous withanimal health, the effects of probiotic Slab51® on the morphology and the glycan composition of guineafowlintestine were examined.
33668637	4	14	theme	high-mannose	1265:1276	arg1	glycans					1287:1293	high-mannose N-linked glycans	1265:1293	high-mannose N-linked glycans	1265:1293	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	0	15	theme	Probiotic	129:137	arg1	Slab51®					139:145	the Multi-Strain Probiotic Slab51®	112:145	the Multi-Strain Probiotic Slab51®	112:145	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.
33668637	4	16	theme	crypt	1533:1537	arg1	cells					1539:1543	crypt cells	1533:1543	crypt cells containing sulfate and non-sulfated acidic glycans	1533:1594	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	17	from	decrease	1449:1456	arg1	sulfoglycans					1340:1351	GCs-secreting sulfoglycans	1326:1351	GCs-secreting sulfoglycans in the ileum	1326:1364	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	17	from	decrease	1449:1456	arg1	GCs					1305:1307	villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs	871:1307	villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs	871:1307	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	5	18	theme	gut	1736:1738	arg1	health					1740:1745	gut health	1736:1745	gut health	1736:1745	Overall, dietary Slab51® induces morphological and region-specific changes in glycoprotein composition of guinea fowl intestine, promoting gut health.
33668637	4	19	theme	crypt	940:944	arg1	depth					946:950	crypt depth	940:950	crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum)	940:1004	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	20	from	<	888:888	arg1	duodenum					899:906	duodenum	899:906	duodenum	899:906	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	20	from	<	888:888	arg1	ileum					912:916	ileum	912:916	ileum	912:916	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	2	21	theme	grow-out	473:480	arg1	cycle.Birds					482:492	the grow-out cycle.Birds	469:492	the grow-out cycle.Birds	469:492	The probiotics were added in drinking water (2 × 1011 UFC/L) throughout the grow-out cycle.Birds were individually weighed andslaughtered after four months.
33668637	0	22	from	Morphology	14:23	arg1	Intestine					99:107	Farmed Guinea Fowl (Numida meleagris) Intestine	61:107	Farmed Guinea Fowl (Numida meleagris) Intestine	61:107	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.
33668637	4	23	from	increase	1231:1238	arg1	glycans					1287:1293	high-mannose N-linked glycans	1265:1293	high-mannose N-linked glycans	1265:1293	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	23	from	increase	1231:1238	arg1	α2,6-sialoglycans					1243:1259	α2,6-sialoglycans	1243:1259	α2,6-sialoglycans	1243:1259	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	0	24	theme	Guinea	68:73	arg1	Fowl					75:78	Farmed Guinea Fowl	61:78	Farmed Guinea Fowl (Numida meleagris) Intestine	61:107	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.
33668637	0	24	theme	Guinea	68:73	arg1	meleagris					88:96	Numida meleagris	81:96	Numida meleagris	81:96	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.
33668637	4	25	theme	GCs-secreting	1326:1338	arg1	sulfoglycans					1340:1351	GCs-secreting sulfoglycans	1326:1351	GCs-secreting sulfoglycans in the ileum	1326:1364	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	26	theme	control	801:807	arg1	samples					809:815	control samples	801:815	control samples	801:815	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	27	from	increase	859:866	arg1	sulfoglycans					1340:1351	GCs-secreting sulfoglycans	1326:1351	GCs-secreting sulfoglycans in the ileum	1326:1364	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	27	from	increase	859:866	arg1	GCs					1305:1307	villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs	871:1307	villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs	871:1307	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	28	dep	<	888:888	arg1	<					921:921	p < 0.05	919:926	p < 0.001 in duodenum and ileum; p < 0.05 in caecum	886:936	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	2	29	theme	×	444:444	arg1	UFC/L					451:455	2 × 1011 UFC/L	442:455	2 × 1011 UFC/L	442:455	The probiotics were added in drinking water (2 × 1011 UFC/L) throughout the grow-out cycle.Birds were individually weighed andslaughtered after four months.
33668637	2	29	theme	×	444:444	arg1	water					435:439	drinking water	426:439	drinking water (2 × 1011 UFC/L)	426:456	The probiotics were added in drinking water (2 × 1011 UFC/L) throughout the grow-out cycle.Birds were individually weighed andslaughtered after four months.
33668637	4	30	theme	non-sulfated	1568:1579	arg1	glycans					1588:1594	non-sulfated acidic glycans	1568:1594	non-sulfated acidic glycans	1568:1594	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	31	from	increase	1371:1378	arg1	glycans					1423:1429	high-mannose N-linked glycans	1401:1429	high-mannose N-linked glycans	1401:1429	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	31	from	increase	1371:1378	arg1	Galβl,3GalNAc					1383:1395	Galβl,3GalNAc	1383:1395	Galβl,3GalNAc	1383:1395	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	32	from	GCs	1206:1208	arg1	duodenum					1217:1224	the duodenum	1213:1224	the duodenum	1213:1224	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	2	33	theme	drinking	426:433	arg1	UFC/L					451:455	2 × 1011 UFC/L	442:455	2 × 1011 UFC/L	442:455	The probiotics were added in drinking water (2 × 1011 UFC/L) throughout the grow-out cycle.Birds were individually weighed andslaughtered after four months.
33668637	2	33	theme	drinking	426:433	arg1	water					435:439	drinking water	426:439	drinking water (2 × 1011 UFC/L)	426:456	The probiotics were added in drinking water (2 × 1011 UFC/L) throughout the grow-out cycle.Birds were individually weighed andslaughtered after four months.
33668637	0	34	theme	Morphology	14:23	arg1	Modulation					0:9	Modulation	0:9	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.	0:146	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.
33668637	4	35	theme	αl,2-fucosylated	1171:1186	arg1	glycans					1188:1194	αl,2-fucosylated glycans	1171:1194	αl,2-fucosylated glycans	1171:1194	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	0	36	theme	Glycan	29:34	arg1	Composition					36:46	Glycan Composition	29:46	Glycan Composition	29:46	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.
33668637	1	37	theme	probiotic	296:304	arg1	Slab51®					306:312	probiotic Slab51®	296:312	probiotic Slab51®	296:312	Probiotics have become highly recognized as supplements for poultry.Since gut health can be considered synonymous withanimal health, the effects of probiotic Slab51® on the morphology and the glycan composition of guineafowlintestine were examined.
33668637	4	38	theme	secretory	1196:1204	arg1	GCs					1206:1208	villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs	871:1208	villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans	871:1293	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	5	39	theme	morphological	1630:1642	arg1	changes					1664:1670	morphological and region-specific changes	1630:1670	morphological and region-specific changes in glycoprotein composition of guinea fowl intestine	1630:1723	Overall, dietary Slab51® induces morphological and region-specific changes in glycoprotein composition of guinea fowl intestine, promoting gut health.
33668637	4	40	dep	height	878:883	arg1	<					888:888	p < 0.001	886:894	p < 0.001 in duodenum and ileum; p < 0.05 in caecum	886:936	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	41	from	<	955:955	arg1	caecum					979:984	caecum	979:984	caecum	979:984	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	41	from	<	955:955	arg1	duodenum					966:973	duodenum	966:973	duodenum	966:973	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	3	42	theme	glycohistochemical	684:701	arg1	results					715:721	morphological, morphometric, conventional and lectin glycohistochemical studies.The results	631:721	morphological, morphometric, conventional and lectin glycohistochemical studies.The results were analyzed for statistical significance by Student's t test	631:784	Samples from the duodenum, ileum and caecum were collected and processed for morphological, morphometric, conventional and lectin glycohistochemical studies.The results were analyzed for statistical significance by Student's t test.
33668637	5	43	theme	region-specific	1648:1662	arg1	changes					1664:1670	morphological and region-specific changes	1630:1670	morphological and region-specific changes in glycoprotein composition of guinea fowl intestine	1630:1723	Overall, dietary Slab51® induces morphological and region-specific changes in glycoprotein composition of guinea fowl intestine, promoting gut health.
33668637	1	44	theme	gut	222:224	arg1	health					226:231	poultry.Since gut health	208:231	poultry.Since gut health	208:231	Probiotics have become highly recognized as supplements for poultry.Since gut health can be considered synonymous withanimal health, the effects of probiotic Slab51® on the morphology and the glycan composition of guineafowlintestine were examined.
33668637	4	45	theme	goblet	1010:1015	arg1	GCs					1024:1026	GCs	1024:1026	GCs	1024:1026	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	45	theme	goblet	1010:1015	arg1	cells					1017:1021	goblet cells	1010:1021	goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans	1010:1194	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	46	theme	N-linked	1414:1421	arg1	glycans					1423:1429	high-mannose N-linked glycans	1401:1429	high-mannose N-linked glycans	1401:1429	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	0	47	from	Intestine	99:107	arg1	Morphology					14:23	Morphology	14:23	Morphology	14:23	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.
33668637	0	47	from	Intestine	99:107	arg1	Composition					36:46	Glycan Composition	29:46	Glycan Composition	29:46	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.
33668637	4	48	theme	secretory	1431:1439	arg1	increase					859:866	(1) significant increase	843:866	(1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs	843:1307	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	5	49	gly	glycoprotein	1675:1686	arg1	glycoprotein					1675:1686	glycoprotein composition	1675:1698	glycoprotein composition of guinea fowl intestine	1675:1723	Overall, dietary Slab51® induces morphological and region-specific changes in glycoprotein composition of guinea fowl intestine, promoting gut health.
33668637	4	50	dep	<	955:955	arg1	<					989:989	p < 0.05	987:994	p < 0.05 in ileum	987:1003	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	5	51	theme	guinea	1703:1708	arg1	intestine					1715:1723	guinea fowl intestine	1703:1723	guinea fowl intestine	1703:1723	Overall, dietary Slab51® induces morphological and region-specific changes in glycoprotein composition of guinea fowl intestine, promoting gut health.
33668637	1	52	theme	withanimal	262:271	arg1	health					273:278	synonymous withanimal health	251:278	synonymous withanimal health	251:278	Probiotics have become highly recognized as supplements for poultry.Since gut health can be considered synonymous withanimal health, the effects of probiotic Slab51® on the morphology and the glycan composition of guineafowlintestine were examined.
33668637	4	53	theme	p	886:886	arg1	<					888:888	p < 0.001	886:894	p < 0.001 in duodenum and ileum; p < 0.05 in caecum	886:936	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	54	from	<	989:989	arg1	ileum					999:1003	ileum	999:1003	ileum	999:1003	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	1	55	from	effects	285:291	arg1	morphology					321:330	the morphology	317:330	the morphology	317:330	Probiotics have become highly recognized as supplements for poultry.Since gut health can be considered synonymous withanimal health, the effects of probiotic Slab51® on the morphology and the glycan composition of guineafowlintestine were examined.
33668637	1	55	from	effects	285:291	arg1	composition					347:357	the glycan composition	336:357	the glycan composition of guineafowlintestine	336:380	Probiotics have become highly recognized as supplements for poultry.Since gut health can be considered synonymous withanimal health, the effects of probiotic Slab51® on the morphology and the glycan composition of guineafowlintestine were examined.
33668637	5	56	theme	intestine	1715:1723	arg1	composition					1688:1698	glycoprotein composition	1675:1698	glycoprotein composition of guinea fowl intestine	1675:1723	Overall, dietary Slab51® induces morphological and region-specific changes in glycoprotein composition of guinea fowl intestine, promoting gut health.
33668637	4	57	theme	significant	847:857	arg1	increase					859:866	(1) significant increase	843:866	(1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs	843:1307	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	58	theme	p	1041:1041	arg1	villus					1033:1038	villus	1033:1038	villus (p < 0.001) in all investigated tracts	1033:1077	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	58	theme	p	1041:1041	arg1	<					1043:1043	p < 0.001	1041:1049	p < 0.001	1041:1049	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	3	59	theme	statistical	741:751	arg1	significance					753:764	statistical significance	741:764	statistical significance	741:764	Samples from the duodenum, ileum and caecum were collected and processed for morphological, morphometric, conventional and lectin glycohistochemical studies.The results were analyzed for statistical significance by Student's t test.
33668637	4	60	from	villus	1033:1038	arg1	tracts					1072:1077	all investigated tracts	1055:1077	all investigated tracts	1055:1077	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	61	theme	N-linked	1278:1285	arg1	glycans					1287:1293	high-mannose N-linked glycans	1265:1293	high-mannose N-linked glycans	1265:1293	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	5	62	theme	glycoprotein	1675:1686	arg1	composition					1688:1698	glycoprotein composition	1675:1698	glycoprotein composition of guinea fowl intestine	1675:1723	Overall, dietary Slab51® induces morphological and region-specific changes in glycoprotein composition of guinea fowl intestine, promoting gut health.
33668637	4	63	theme	secretory	1295:1303	arg1	GCs					1305:1307	villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs	871:1307	villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs	871:1307	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	0	64	theme	Multi-Strain	116:127	arg1	Slab51®					139:145	the Multi-Strain Probiotic Slab51®	112:145	the Multi-Strain Probiotic Slab51®	112:145	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.
33668637	4	65	dep	increase	1231:1238	arg1	3					1228:1228	3	1228:1228	3	1228:1228	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	66	theme	p	919:919	arg1	<					921:921	p < 0.05	919:926	p < 0.001 in duodenum and ileum; p < 0.05 in caecum	886:936	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	67	theme	investigated	1059:1070	arg1	tracts					1072:1077	all investigated tracts	1055:1077	all investigated tracts	1055:1077	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	68	theme	cells	1539:1543	arg1	numbers					1522:1528	the numbers	1518:1528	the numbers of crypt cells containing sulfate and non-sulfated acidic glycans	1518:1594	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	69	link	N-linked	1278:1285	arg1	glycans					1287:1293	high-mannose N-linked glycans	1265:1293	high-mannose N-linked glycans	1265:1293	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	70	gly	αl,2-fucosylated	1171:1186	arg1	glycans					1188:1194	αl,2-fucosylated glycans	1171:1194	αl,2-fucosylated glycans	1171:1194	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	3	71	from	duodenum	571:578	arg1	Samples					554:560	Samples	554:560	Samples from the duodenum, ileum and caecum	554:596	Samples from the duodenum, ileum and caecum were collected and processed for morphological, morphometric, conventional and lectin glycohistochemical studies.The results were analyzed for statistical significance by Student's t test.
33668637	0	72	theme	Farmed	61:66	arg1	Fowl					75:78	Farmed Guinea Fowl	61:78	Farmed Guinea Fowl (Numida meleagris) Intestine	61:107	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.
33668637	0	72	theme	Farmed	61:66	arg1	meleagris					88:96	Numida meleagris	81:96	Numida meleagris	81:96	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.
33668637	4	73	dep	increase	1371:1378	arg1	4					1368:1368	4	1368:1368	4	1368:1368	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	0	74	theme	Fowl	75:78	arg1	Intestine					99:107	Farmed Guinea Fowl (Numida meleagris) Intestine	61:107	Farmed Guinea Fowl (Numida meleagris) Intestine	61:107	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.
33668637	3	75	dep	duodenum	571:578	arg1	the					567:569	the	567:569	the	567:569	Samples from the duodenum, ileum and caecum were collected and processed for morphological, morphometric, conventional and lectin glycohistochemical studies.The results were analyzed for statistical significance by Student's t test.
33668637	4	76	dep	GCs	1206:1208	arg1	increase					1231:1238	(3) increase	1227:1238	villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans	871:1293	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	77	theme	acidic	1581:1586	arg1	glycans					1588:1594	non-sulfated acidic glycans	1568:1594	non-sulfated acidic glycans	1568:1594	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	0	78	theme	Numida	81:86	arg1	Fowl					75:78	Farmed Guinea Fowl	61:78	Farmed Guinea Fowl (Numida meleagris) Intestine	61:107	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.
33668637	0	78	theme	Numida	81:86	arg1	meleagris					88:96	Numida meleagris	81:96	Numida meleagris	81:96	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.
33668637	1	79	theme	glycan	340:345	arg1	composition					347:357	the glycan composition	336:357	the glycan composition of guineafowlintestine	336:380	Probiotics have become highly recognized as supplements for poultry.Since gut health can be considered synonymous withanimal health, the effects of probiotic Slab51® on the morphology and the glycan composition of guineafowlintestine were examined.
33668637	0	80	from	Mucins	51:56	arg1	Intestine					99:107	Farmed Guinea Fowl (Numida meleagris) Intestine	61:107	Farmed Guinea Fowl (Numida meleagris) Intestine	61:107	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.
33668637	0	81	from	Composition	36:46	arg1	Intestine					99:107	Farmed Guinea Fowl (Numida meleagris) Intestine	61:107	Farmed Guinea Fowl (Numida meleagris) Intestine	61:107	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.
33668637	4	82	from	increase	1084:1091	arg1	glycans					1188:1194	αl,2-fucosylated glycans	1171:1194	αl,2-fucosylated glycans	1171:1194	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	82	from	increase	1084:1091	arg1	Galβl,3GalNAc					1131:1143	Galβl,3GalNAc	1131:1143	Galβl,3GalNAc	1131:1143	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	82	from	increase	1084:1091	arg1	galactoseβl,3N-acetylgalacyosamine					1096:1129	galactoseβl,3N-acetylgalacyosamine	1096:1129	galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans	1096:1165	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	3	83	from	ileum	581:585	arg1	Samples					554:560	Samples	554:560	Samples from the duodenum, ileum and caecum	554:596	Samples from the duodenum, ileum and caecum were collected and processed for morphological, morphometric, conventional and lectin glycohistochemical studies.The results were analyzed for statistical significance by Student's t test.
33668637	4	84	dep	increase	859:866	arg1	1					844:844	1	844:844	1	844:844	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	84	dep	increase	859:866	arg1	GCs					1441:1443	GCs	1441:1443	GCs	1441:1443	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	2	85	dep	weighed	512:518	arg1	andslaughtered					520:533	andslaughtered	520:533	were individually weighed andslaughtered after four months	494:551	The probiotics were added in drinking water (2 × 1011 UFC/L) throughout the grow-out cycle.Birds were individually weighed andslaughtered after four months.
33668637	4	86	link	N-linked	1414:1421	arg1	glycans					1423:1429	high-mannose N-linked glycans	1401:1429	high-mannose N-linked glycans	1401:1429	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	3	87	theme	morphometric	646:657	arg1	results					715:721	morphological, morphometric, conventional and lectin glycohistochemical studies.The results	631:721	morphological, morphometric, conventional and lectin glycohistochemical studies.The results were analyzed for statistical significance by Student's t test	631:784	Samples from the duodenum, ileum and caecum were collected and processed for morphological, morphometric, conventional and lectin glycohistochemical studies.The results were analyzed for statistical significance by Student's t test.
33668637	3	88	from	caecum	591:596	arg1	Samples					554:560	Samples	554:560	Samples from the duodenum, ileum and caecum	554:596	Samples from the duodenum, ileum and caecum were collected and processed for morphological, morphometric, conventional and lectin glycohistochemical studies.The results were analyzed for statistical significance by Student's t test.
33668637	0	89	theme	Composition	36:46	arg1	Modulation					0:9	Modulation	0:9	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.	0:146	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.
33668637	4	90	theme	p	953:953	arg1	<					955:955	p < 0.001	953:961	p < 0.001 in duodenum and caecum	953:984	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	3	91	theme	morphological	631:643	arg1	results					715:721	morphological, morphometric, conventional and lectin glycohistochemical studies.The results	631:721	morphological, morphometric, conventional and lectin glycohistochemical studies.The results were analyzed for statistical significance by Student's t test	631:784	Samples from the duodenum, ileum and caecum were collected and processed for morphological, morphometric, conventional and lectin glycohistochemical studies.The results were analyzed for statistical significance by Student's t test.
33668637	0	92	theme	Mucins	51:56	arg1	Morphology					14:23	Morphology	14:23	Morphology	14:23	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.
33668637	0	92	theme	Mucins	51:56	arg1	Composition					36:46	Glycan Composition	29:46	Glycan Composition	29:46	Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®.
33668637	1	93	theme	Slab51®	306:312	arg1	effects					285:291	the effects	281:291	the effects of probiotic Slab51® on the morphology and the glycan composition of guineafowlintestine	281:380	Probiotics have become highly recognized as supplements for poultry.Since gut health can be considered synonymous withanimal health, the effects of probiotic Slab51® on the morphology and the glycan composition of guineafowlintestine were examined.
33668637	1	94	theme	guineafowlintestine	362:380	arg1	morphology					321:330	the morphology	317:330	the morphology	317:330	Probiotics have become highly recognized as supplements for poultry.Since gut health can be considered synonymous withanimal health, the effects of probiotic Slab51® on the morphology and the glycan composition of guineafowlintestine were examined.
33668637	1	94	theme	guineafowlintestine	362:380	arg1	composition					347:357	the glycan composition	336:357	the glycan composition of guineafowlintestine	336:380	Probiotics have become highly recognized as supplements for poultry.Since gut health can be considered synonymous withanimal health, the effects of probiotic Slab51® on the morphology and the glycan composition of guineafowlintestine were examined.
33668637	2	95	theme	1011	446:449	arg1	UFC/L					451:455	2 × 1011 UFC/L	442:455	2 × 1011 UFC/L	442:455	The probiotics were added in drinking water (2 × 1011 UFC/L) throughout the grow-out cycle.Birds were individually weighed andslaughtered after four months.
33668637	2	95	theme	1011	446:449	arg1	water					435:439	drinking water	426:439	drinking water (2 × 1011 UFC/L)	426:456	The probiotics were added in drinking water (2 × 1011 UFC/L) throughout the grow-out cycle.Birds were individually weighed andslaughtered after four months.
33668637	3	96	theme	conventional	660:671	arg1	results					715:721	morphological, morphometric, conventional and lectin glycohistochemical studies.The results	631:721	morphological, morphometric, conventional and lectin glycohistochemical studies.The results were analyzed for statistical significance by Student's t test	631:784	Samples from the duodenum, ileum and caecum were collected and processed for morphological, morphometric, conventional and lectin glycohistochemical studies.The results were analyzed for statistical significance by Student's t test.
33668637	5	97	theme	dietary	1606:1612	arg1	Slab51®					1614:1620	dietary Slab51®	1606:1620	dietary Slab51®	1606:1620	Overall, dietary Slab51® induces morphological and region-specific changes in glycoprotein composition of guinea fowl intestine, promoting gut health.
33668637	4	98	contain	containing	1545:1554	arg2	glycans					1588:1594	non-sulfated acidic glycans	1568:1594	non-sulfated acidic glycans	1568:1594	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	98	contain	containing	1545:1554	arg2	sulfate					1556:1562	sulfate	1556:1562	sulfate	1556:1562	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	98	contain	containing	1545:1554	arg1	cells					1539:1543	crypt cells	1533:1543	crypt cells containing sulfate and non-sulfated acidic glycans	1533:1594	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	99	from	depth	946:950	arg1	glycans					1188:1194	αl,2-fucosylated glycans	1171:1194	αl,2-fucosylated glycans	1171:1194	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	99	from	depth	946:950	arg1	Galβl,3GalNAc					1131:1143	Galβl,3GalNAc	1131:1143	Galβl,3GalNAc	1131:1143	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	99	from	depth	946:950	arg1	galactoseβl,3N-acetylgalacyosamine					1096:1129	galactoseβl,3N-acetylgalacyosamine	1096:1129	galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans	1096:1165	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	100	from	height	878:883	arg1	glycans					1188:1194	αl,2-fucosylated glycans	1171:1194	αl,2-fucosylated glycans	1171:1194	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	100	from	height	878:883	arg1	Galβl,3GalNAc					1131:1143	Galβl,3GalNAc	1131:1143	Galβl,3GalNAc	1131:1143	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	100	from	height	878:883	arg1	galactoseβl,3N-acetylgalacyosamine					1096:1129	galactoseβl,3N-acetylgalacyosamine	1096:1129	galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans	1096:1165	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	101	theme	probiotic	818:826	arg1	group					828:832	probiotic group	818:832	probiotic group	818:832	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	102	from	increase	1506:1513	arg1	numbers					1522:1528	the numbers	1518:1528	the numbers of crypt cells containing sulfate and non-sulfated acidic glycans	1518:1594	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33668637	4	103	theme	high-mannose	1401:1412	arg1	glycans					1423:1429	high-mannose N-linked glycans	1401:1429	high-mannose N-linked glycans	1401:1429	Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum; p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans.
33966844	7	0	from	drug	1027:1030	arg1	field					1047:1051	biomedicine field	1035:1051	biomedicine field	1035:1051	These results suggested that RLP50-2 could be developed as a potential immunomodulatory agent or antitumor candidate drug in biomedicine field.
33966844	4	1	theme	-α-D-Galp-	566:575	arg1	-β-D-Xylp-					602:611	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	-β-D-Xylp-	602:611	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	4	2	theme	-α-D-Glcp-	510:519	arg1	-β-D-Xylp-					602:611	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	-β-D-Xylp-	602:611	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	6	3	theme	tumor	844:848	arg1	cell					850:853	tumor cell proliferation and migration	844:881	tumor cell proliferation and migration	844:881	Moreover, RLP50-2 showed significantly antitumor activities by inhibiting tumor cell proliferation and migration and blocking angiogenesis.
33966844	0	4	from	analysis	11:18	arg1	fruits					80:85	the fruits	76:85	the fruits of Rosa laevigata	76:103	Structural analysis and biological effects of a neutral polysaccharide from the fruits of Rosa laevigata.
33966844	5	5	contain	had	705:707	arg1	RLP50-2					697:703	RLP50-2	697:703	RLP50-2	697:703	Biological assays showed that RLP50-2 had immunomodulatory activities using cell and zebrafish models.
33966844	5	5	contain	had	705:707	arg2	activities					726:735	immunomodulatory activities	709:735	immunomodulatory activities using cell and zebrafish models	709:767	Biological assays showed that RLP50-2 had immunomodulatory activities using cell and zebrafish models.
33966844	5	6	theme	cell	743:746	arg1	models					762:767	cell and zebrafish models	743:767	models	762:767	Biological assays showed that RLP50-2 had immunomodulatory activities using cell and zebrafish models.
33966844	4	7	theme	→5	449:450	arg1	-β-D-Xylp-					602:611	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	-β-D-Xylp-	602:611	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	0	8	theme	Rosa	90:93	arg1	laevigata					95:103	Rosa laevigata	90:103	Rosa laevigata	90:103	Structural analysis and biological effects of a neutral polysaccharide from the fruits of Rosa laevigata.
33966844	6	9	dep	cell	850:853	arg1	migration					873:881	migration	873:881	migration	873:881	Moreover, RLP50-2 showed significantly antitumor activities by inhibiting tumor cell proliferation and migration and blocking angiogenesis.
33966844	6	9	dep	cell	850:853	arg1	proliferation					855:867	proliferation	855:867	proliferation	855:867	Moreover, RLP50-2 showed significantly antitumor activities by inhibiting tumor cell proliferation and migration and blocking angiogenesis.
33966844	7	10	theme	potential	971:979	arg1	RLP50-2					939:945	RLP50-2	939:945	RLP50-2	939:945	These results suggested that RLP50-2 could be developed as a potential immunomodulatory agent or antitumor candidate drug in biomedicine field.
33966844	7	10	theme	potential	971:979	arg1	agent					998:1002	a potential immunomodulatory agent	969:1002	a potential immunomodulatory agent	969:1002	These results suggested that RLP50-2 could be developed as a potential immunomodulatory agent or antitumor candidate drug in biomedicine field.
33966844	4	11	theme	1→	521:522	arg1	-β-D-Xylp-					602:611	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	-β-D-Xylp-	602:611	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	4	12	theme	1→	463:464	arg1	-β-D-Xylp-					602:611	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	-β-D-Xylp-	602:611	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	7	13	theme	immunomodulatory	981:996	arg1	RLP50-2					939:945	RLP50-2	939:945	RLP50-2	939:945	These results suggested that RLP50-2 could be developed as a potential immunomodulatory agent or antitumor candidate drug in biomedicine field.
33966844	7	13	theme	immunomodulatory	981:996	arg1	agent					998:1002	a potential immunomodulatory agent	969:1002	a potential immunomodulatory agent	969:1002	These results suggested that RLP50-2 could be developed as a potential immunomodulatory agent or antitumor candidate drug in biomedicine field.
33966844	4	14	theme	Structural	394:403	arg1	analysis					405:412	Structural analysis	394:412	Structural analysis	394:412	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	4	15	theme	-β-D-Glcp-	548:557	arg1	-β-D-Xylp-					602:611	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	-β-D-Xylp-	602:611	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	0	16	theme	laevigata	95:103	arg1	fruits					80:85	the fruits	76:85	the fruits of Rosa laevigata	76:103	Structural analysis and biological effects of a neutral polysaccharide from the fruits of Rosa laevigata.
33966844	7	17	theme	antitumor	1007:1015	arg1	drug					1027:1030	antitumor candidate drug	1007:1030	antitumor candidate drug in biomedicine field	1007:1051	These results suggested that RLP50-2 could be developed as a potential immunomodulatory agent or antitumor candidate drug in biomedicine field.
33966844	7	17	theme	antitumor	1007:1015	arg1	RLP50-2					939:945	RLP50-2	939:945	RLP50-2	939:945	These results suggested that RLP50-2 could be developed as a potential immunomodulatory agent or antitumor candidate drug in biomedicine field.
33966844	7	18	from	agent	998:1002	arg1	field					1047:1051	biomedicine field	1035:1051	biomedicine field	1035:1051	These results suggested that RLP50-2 could be developed as a potential immunomodulatory agent or antitumor candidate drug in biomedicine field.
33966844	0	19	from	effects	35:41	arg1	fruits					80:85	the fruits	76:85	the fruits of Rosa laevigata	76:103	Structural analysis and biological effects of a neutral polysaccharide from the fruits of Rosa laevigata.
33966844	4	20	theme	-β-D-Galp-	584:593	arg1	-β-D-Xylp-					602:611	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	-β-D-Xylp-	602:611	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	4	21	theme	1→	559:560	arg1	-β-D-Xylp-					602:611	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	-β-D-Xylp-	602:611	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	0	22	theme	Structural	0:9	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis	0:18	Structural analysis and biological effects of a neutral polysaccharide from the fruits of Rosa laevigata.
33966844	4	23	theme	1→	503:504	arg1	-β-D-Xylp-					602:611	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	-β-D-Xylp-	602:611	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	0	24	theme	biological	24:33	arg1	effects					35:41	biological effects	24:41	biological effects	24:41	Structural analysis and biological effects of a neutral polysaccharide from the fruits of Rosa laevigata.
33966844	4	25	theme	1→	539:540	arg1	-β-D-Xylp-					602:611	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	-β-D-Xylp-	602:611	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	6	26	theme	antitumor	809:817	arg1	activities					819:828	significantly antitumor activities	795:828	significantly antitumor activities	795:828	Moreover, RLP50-2 showed significantly antitumor activities by inhibiting tumor cell proliferation and migration and blocking angiogenesis.
33966844	0	27	from	fruits	80:85	arg1	polysaccharide					56:69	a neutral polysaccharide	46:69	a neutral polysaccharide from the fruits of Rosa laevigata	46:103	Structural analysis and biological effects of a neutral polysaccharide from the fruits of Rosa laevigata.
33966844	0	27	from	fruits	80:85	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis	0:18	Structural analysis and biological effects of a neutral polysaccharide from the fruits of Rosa laevigata.
33966844	0	27	from	fruits	80:85	arg1	effects					35:41	biological effects	24:41	biological effects	24:41	Structural analysis and biological effects of a neutral polysaccharide from the fruits of Rosa laevigata.
33966844	5	28	theme	immunomodulatory	709:724	arg1	activities					726:735	immunomodulatory activities	709:735	immunomodulatory activities using cell and zebrafish models	709:767	Biological assays showed that RLP50-2 had immunomodulatory activities using cell and zebrafish models.
33966844	5	29	theme	zebrafish	752:760	arg1	models					762:767	cell and zebrafish models	743:767	models	762:767	Biological assays showed that RLP50-2 had immunomodulatory activities using cell and zebrafish models.
33966844	2	30	theme	absolute	221:228	arg1	weight					240:245	The absolute molecular weight	217:245	The absolute molecular weight	217:245	The absolute molecular weight was determined as 1.26 × 104 g/mol.
33966844	2	30	theme	absolute	221:228	arg1	g/mol					276:280	1.26 × 104 g/mol	265:280	1.26 × 104 g/mol	265:280	The absolute molecular weight was determined as 1.26 × 104 g/mol.
33966844	5	31	theme	Biological	667:676	arg1	assays					678:683	Biological assays	667:683	Biological assays	667:683	Biological assays showed that RLP50-2 had immunomodulatory activities using cell and zebrafish models.
33966844	4	32	theme	-α-L-Araf-	452:461	arg1	-β-D-Xylp-					602:611	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	-β-D-Xylp-	602:611	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	7	33	theme	biomedicine	1035:1045	arg1	field					1047:1051	biomedicine field	1035:1051	biomedicine field	1035:1051	These results suggested that RLP50-2 could be developed as a potential immunomodulatory agent or antitumor candidate drug in biomedicine field.
33966844	4	34	theme	1→	483:484	arg1	-β-D-Xylp-					602:611	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	-β-D-Xylp-	602:611	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	1	35	theme	Rosa	201:204	arg1	laevigata					206:214	Rosa laevigata	201:214	Rosa laevigata	201:214	A neutral water-soluble polysaccharide (RLP50-2) was extracted and purified from the fruits of Rosa laevigata.
33966844	0	36	theme	neutral	48:54	arg1	polysaccharide					56:69	a neutral polysaccharide	46:69	a neutral polysaccharide from the fruits of Rosa laevigata	46:103	Structural analysis and biological effects of a neutral polysaccharide from the fruits of Rosa laevigata.
33966844	3	37	theme	Monosaccharide	283:296	arg1	analysis					310:317	Monosaccharide composition analysis	283:317	Monosaccharide composition analysis	283:317	Monosaccharide composition analysis showed that RLP50-2 mainly consisted of glucose, arabinose, and galactose.
33966844	4	38	theme	terminal	645:652	arg1	β-D-mannose					654:664	terminal β-D-mannose	645:664	terminal β-D-mannose	645:664	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	7	39	theme	candidate	1017:1025	arg1	drug					1027:1030	antitumor candidate drug	1007:1030	antitumor candidate drug in biomedicine field	1007:1051	These results suggested that RLP50-2 could be developed as a potential immunomodulatory agent or antitumor candidate drug in biomedicine field.
33966844	7	39	theme	candidate	1017:1025	arg1	RLP50-2					939:945	RLP50-2	939:945	RLP50-2	939:945	These results suggested that RLP50-2 could be developed as a potential immunomodulatory agent or antitumor candidate drug in biomedicine field.
33966844	4	40	theme	-α-L-Araf-	492:501	arg1	-β-D-Xylp-					602:611	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	-β-D-Xylp-	602:611	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	3	41	theme	composition	298:308	arg1	analysis					310:317	Monosaccharide composition analysis	283:317	Monosaccharide composition analysis	283:317	Monosaccharide composition analysis showed that RLP50-2 mainly consisted of glucose, arabinose, and galactose.
33966844	1	42	theme	neutral	108:114	arg1	RLP50-2					146:152	RLP50-2	146:152	RLP50-2	146:152	A neutral water-soluble polysaccharide (RLP50-2) was extracted and purified from the fruits of Rosa laevigata.
33966844	1	42	theme	neutral	108:114	arg1	polysaccharide					130:143	A neutral water-soluble polysaccharide	106:143	A neutral water-soluble polysaccharide (RLP50-2)	106:153	A neutral water-soluble polysaccharide (RLP50-2) was extracted and purified from the fruits of Rosa laevigata.
33966844	1	43	theme	water-soluble	116:128	arg1	RLP50-2					146:152	RLP50-2	146:152	RLP50-2	146:152	A neutral water-soluble polysaccharide (RLP50-2) was extracted and purified from the fruits of Rosa laevigata.
33966844	1	43	theme	water-soluble	116:128	arg1	polysaccharide					130:143	A neutral water-soluble polysaccharide	106:143	A neutral water-soluble polysaccharide (RLP50-2)	106:153	A neutral water-soluble polysaccharide (RLP50-2) was extracted and purified from the fruits of Rosa laevigata.
33966844	1	44	theme	laevigata	206:214	arg1	fruits					191:196	the fruits	187:196	the fruits of Rosa laevigata	187:214	A neutral water-soluble polysaccharide (RLP50-2) was extracted and purified from the fruits of Rosa laevigata.
33966844	4	45	theme	terminal	617:624	arg1	α-L-arabinose					626:638	terminal α-L-arabinose	617:638	terminal α-L-arabinose	617:638	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	4	46	dep	-β-D-Xylp-	602:611	arg1	→3,6					543:546	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	→3,6	543:546	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	4	46	dep	-β-D-Xylp-	602:611	arg1	→4					563:564	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	→4	563:564	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	4	46	dep	-β-D-Xylp-	602:611	arg1	→4					507:508	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	→4	507:508	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	4	46	dep	-β-D-Xylp-	602:611	arg1	→6					525:526	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	→6	525:526	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	4	46	dep	-β-D-Xylp-	602:611	arg1	→2,5					467:470	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	→2,5	467:470	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	4	46	dep	-β-D-Xylp-	602:611	arg1	→3,5					487:490	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	→3,5	487:490	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	4	46	dep	-β-D-Xylp-	602:611	arg1	1→					613:614	1→	613:614	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→	449:614	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	4	47	theme	-α-D-Glcp-	528:537	arg1	-β-D-Xylp-					602:611	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	-β-D-Xylp-	602:611	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	0	48	theme	polysaccharide	56:69	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis	0:18	Structural analysis and biological effects of a neutral polysaccharide from the fruits of Rosa laevigata.
33966844	0	48	theme	polysaccharide	56:69	arg1	effects					35:41	biological effects	24:41	biological effects	24:41	Structural analysis and biological effects of a neutral polysaccharide from the fruits of Rosa laevigata.
33966844	4	49	theme	-α-L-Araf-	472:481	arg1	-β-D-Xylp-					602:611	→5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-	449:611	-β-D-Xylp-	602:611	Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose.
33966844	2	50	theme	molecular	230:238	arg1	weight					240:245	The absolute molecular weight	217:245	The absolute molecular weight	217:245	The absolute molecular weight was determined as 1.26 × 104 g/mol.
33966844	2	50	theme	molecular	230:238	arg1	g/mol					276:280	1.26 × 104 g/mol	265:280	1.26 × 104 g/mol	265:280	The absolute molecular weight was determined as 1.26 × 104 g/mol.
32483582	2	0	theme	defined	533:539	arg1	composition					541:551	a highly biocompatible, animal-free and chemically defined composition	482:551	a highly biocompatible, animal-free and chemically defined composition	482:551	STM-148B's putative game-changing design characteristics include a highly biocompatible, animal-free and chemically defined composition, reproducibility of manufacture and ease of formulation.
32483582	2	0	theme	defined	533:539	arg1	ease					589:592	ease	589:592	ease of formulation	589:607	STM-148B's putative game-changing design characteristics include a highly biocompatible, animal-free and chemically defined composition, reproducibility of manufacture and ease of formulation.
32483582	2	0	theme	defined	533:539	arg1	reproducibility					554:568	reproducibility	554:568	reproducibility of manufacture	554:583	STM-148B's putative game-changing design characteristics include a highly biocompatible, animal-free and chemically defined composition, reproducibility of manufacture and ease of formulation.
32483582	3	1	dep	rendered	818:825	arg1	such					779:782	such	779:782	such	779:782	STM-148B undergoes rapid ionic crossinking by physiologically relevant mono and divalent cations to form stable 3D hydrogels that possess excellent tissue adhesiveness, such that additional fixation aids are rendered superfluous.
32483582	4	2	theme	extracellular	973:985	arg1	markers					994:1000	healthy chondrogenic extracellular matrix markers	952:1000	healthy chondrogenic extracellular matrix markers	952:1000	STM-148B hydrogels maintain viability of mammalian cells and further promote up-regulation of the expression of healthy chondrogenic extracellular matrix markers upon stimulation.
32483582	3	3	theme	fixation	800:807	arg1	aids					809:812	additional fixation aids	789:812	additional fixation aids	789:812	STM-148B undergoes rapid ionic crossinking by physiologically relevant mono and divalent cations to form stable 3D hydrogels that possess excellent tissue adhesiveness, such that additional fixation aids are rendered superfluous.
32483582	5	4	theme	tissue-engineering	1263:1280	arg1	therapies					1282:1290	tissue-engineering therapies	1263:1290	tissue-engineering therapies for treatment of focal cartilage lesions	1263:1331	STM-148B is currently undergoing pre-clinical safety and efficacy assessment as a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage and may represent a valuable addition to the armamentarium of tissue-engineering therapies for treatment of focal cartilage lesions.
32483582	3	5	theme	tissue	758:763	arg1	adhesiveness					765:776	excellent tissue adhesiveness	748:776	excellent tissue adhesiveness	748:776	STM-148B undergoes rapid ionic crossinking by physiologically relevant mono and divalent cations to form stable 3D hydrogels that possess excellent tissue adhesiveness, such that additional fixation aids are rendered superfluous.
32483582	1	6	from	retention	350:358	arg1	context					367:373	the context	363:373	the context of cartilage repair	363:393	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B) with improved physicochemical and biological characteristics, suitable for minimally invasive cell delivery and retention in the context of cartilage repair, is herein presented.
32483582	4	7	theme	matrix	987:992	arg1	markers					994:1000	healthy chondrogenic extracellular matrix markers	952:1000	healthy chondrogenic extracellular matrix markers	952:1000	STM-148B hydrogels maintain viability of mammalian cells and further promote up-regulation of the expression of healthy chondrogenic extracellular matrix markers upon stimulation.
32483582	1	8	theme	biological	272:281	arg1	characteristics					283:297	improved physicochemical and biological characteristics	243:297	improved physicochemical and biological characteristics	243:297	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B) with improved physicochemical and biological characteristics, suitable for minimally invasive cell delivery and retention in the context of cartilage repair, is herein presented.
32483582	4	9	theme	markers	994:1000	arg1	expression					938:947	the expression	934:947	the expression of healthy chondrogenic extracellular matrix markers	934:1000	STM-148B hydrogels maintain viability of mammalian cells and further promote up-regulation of the expression of healthy chondrogenic extracellular matrix markers upon stimulation.
32483582	3	10	dep	possess	740:746	arg1	rendered					818:825	rendered	818:825	are rendered superfluous	814:837	STM-148B undergoes rapid ionic crossinking by physiologically relevant mono and divalent cations to form stable 3D hydrogels that possess excellent tissue adhesiveness, such that additional fixation aids are rendered superfluous.
32483582	2	11	theme	manufacture	573:583	arg1	composition					541:551	a highly biocompatible, animal-free and chemically defined composition	482:551	a highly biocompatible, animal-free and chemically defined composition	482:551	STM-148B's putative game-changing design characteristics include a highly biocompatible, animal-free and chemically defined composition, reproducibility of manufacture and ease of formulation.
32483582	2	11	theme	manufacture	573:583	arg1	ease					589:592	ease	589:592	ease of formulation	589:607	STM-148B's putative game-changing design characteristics include a highly biocompatible, animal-free and chemically defined composition, reproducibility of manufacture and ease of formulation.
32483582	2	11	theme	manufacture	573:583	arg1	reproducibility					554:568	reproducibility	554:568	reproducibility of manufacture	554:583	STM-148B's putative game-changing design characteristics include a highly biocompatible, animal-free and chemically defined composition, reproducibility of manufacture and ease of formulation.
32483582	1	12	theme	cartilage	378:386	arg1	repair					388:393	cartilage repair	378:393	cartilage repair	378:393	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B) with improved physicochemical and biological characteristics, suitable for minimally invasive cell delivery and retention in the context of cartilage repair, is herein presented.
32483582	1	13	from	delivery	337:344	arg1	context					367:373	the context	363:373	the context of cartilage repair	363:393	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B) with improved physicochemical and biological characteristics, suitable for minimally invasive cell delivery and retention in the context of cartilage repair, is herein presented.
32483582	4	14	theme	STM-148B	840:847	arg1	hydrogels					849:857	STM-148B hydrogels	840:857	STM-148B hydrogels	840:857	STM-148B hydrogels maintain viability of mammalian cells and further promote up-regulation of the expression of healthy chondrogenic extracellular matrix markers upon stimulation.
32483582	0	15	theme	invasive	119:126	arg1	delivery					128:135	minimally invasive delivery	109:135	minimally invasive delivery	109:135	Synthesis and biological evaluation of a bioinspired, tissue-adhesive gellan gum-based hydrogel designed for minimally invasive delivery and retention of chondrogenic cells.
32483582	1	16	theme	repair	388:393	arg1	context					367:373	the context	363:373	the context of cartilage repair	363:393	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B) with improved physicochemical and biological characteristics, suitable for minimally invasive cell delivery and retention in the context of cartilage repair, is herein presented.
32483582	4	17	theme	expression	938:947	arg1	up-regulation					917:929	up-regulation	917:929	up-regulation of the expression of healthy chondrogenic extracellular matrix markers upon stimulation	917:1017	STM-148B hydrogels maintain viability of mammalian cells and further promote up-regulation of the expression of healthy chondrogenic extracellular matrix markers upon stimulation.
32483582	5	18	theme	medical	1102:1108	arg1	safety					1066:1071	pre-clinical safety and efficacy assessment	1053:1095	safety	1066:1071	STM-148B is currently undergoing pre-clinical safety and efficacy assessment as a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage and may represent a valuable addition to the armamentarium of tissue-engineering therapies for treatment of focal cartilage lesions.
32483582	5	18	theme	medical	1102:1108	arg1	assessment					1086:1095	pre-clinical safety and efficacy assessment	1053:1095	assessment	1086:1095	STM-148B is currently undergoing pre-clinical safety and efficacy assessment as a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage and may represent a valuable addition to the armamentarium of tissue-engineering therapies for treatment of focal cartilage lesions.
32483582	5	18	theme	medical	1102:1108	arg1	device					1110:1115	a medical device	1100:1115	a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage	1100:1199	STM-148B is currently undergoing pre-clinical safety and efficacy assessment as a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage and may represent a valuable addition to the armamentarium of tissue-engineering therapies for treatment of focal cartilage lesions.
32483582	1	19	theme	dopamine-modified	176:192	arg1	hydrogel					218:225	A dopamine-modified, bioinspired gellan gum hydrogel	174:225	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B)	174:236	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B) with improved physicochemical and biological characteristics, suitable for minimally invasive cell delivery and retention in the context of cartilage repair, is herein presented.
32483582	1	19	theme	dopamine-modified	176:192	arg1	STM-148B					228:235	STM-148B	228:235	STM-148B	228:235	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B) with improved physicochemical and biological characteristics, suitable for minimally invasive cell delivery and retention in the context of cartilage repair, is herein presented.
32483582	2	20	theme	biocompatible	491:503	arg1	composition					541:551	a highly biocompatible, animal-free and chemically defined composition	482:551	a highly biocompatible, animal-free and chemically defined composition	482:551	STM-148B's putative game-changing design characteristics include a highly biocompatible, animal-free and chemically defined composition, reproducibility of manufacture and ease of formulation.
32483582	2	20	theme	biocompatible	491:503	arg1	ease					589:592	ease	589:592	ease of formulation	589:607	STM-148B's putative game-changing design characteristics include a highly biocompatible, animal-free and chemically defined composition, reproducibility of manufacture and ease of formulation.
32483582	2	20	theme	biocompatible	491:503	arg1	reproducibility					554:568	reproducibility	554:568	reproducibility of manufacture	554:583	STM-148B's putative game-changing design characteristics include a highly biocompatible, animal-free and chemically defined composition, reproducibility of manufacture and ease of formulation.
32483582	3	21	theme	additional	789:798	arg1	aids					809:812	additional fixation aids	789:812	additional fixation aids	789:812	STM-148B undergoes rapid ionic crossinking by physiologically relevant mono and divalent cations to form stable 3D hydrogels that possess excellent tissue adhesiveness, such that additional fixation aids are rendered superfluous.
32483582	4	22	theme	healthy	952:958	arg1	markers					994:1000	healthy chondrogenic extracellular matrix markers	952:1000	healthy chondrogenic extracellular matrix markers	952:1000	STM-148B hydrogels maintain viability of mammalian cells and further promote up-regulation of the expression of healthy chondrogenic extracellular matrix markers upon stimulation.
32483582	5	23	theme	lesions	1325:1331	arg1	treatment					1296:1304	treatment	1296:1304	treatment of focal cartilage lesions	1296:1331	STM-148B is currently undergoing pre-clinical safety and efficacy assessment as a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage and may represent a valuable addition to the armamentarium of tissue-engineering therapies for treatment of focal cartilage lesions.
32483582	3	24	theme	rapid	629:633	arg1	crossinking					641:651	rapid ionic crossinking	629:651	rapid ionic crossinking by physiologically relevant mono and divalent cations	629:705	STM-148B undergoes rapid ionic crossinking by physiologically relevant mono and divalent cations to form stable 3D hydrogels that possess excellent tissue adhesiveness, such that additional fixation aids are rendered superfluous.
32483582	5	25	theme	therapies	1282:1290	arg1	armamentarium					1246:1258	the armamentarium	1242:1258	the armamentarium of tissue-engineering therapies for treatment of focal cartilage lesions	1242:1331	STM-148B is currently undergoing pre-clinical safety and efficacy assessment as a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage and may represent a valuable addition to the armamentarium of tissue-engineering therapies for treatment of focal cartilage lesions.
32483582	1	26	theme	suitable	300:307	arg1	hydrogel					218:225	A dopamine-modified, bioinspired gellan gum hydrogel	174:225	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B)	174:236	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B) with improved physicochemical and biological characteristics, suitable for minimally invasive cell delivery and retention in the context of cartilage repair, is herein presented.
32483582	1	26	theme	suitable	300:307	arg1	STM-148B					228:235	STM-148B	228:235	STM-148B	228:235	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B) with improved physicochemical and biological characteristics, suitable for minimally invasive cell delivery and retention in the context of cartilage repair, is herein presented.
32483582	3	27	theme	ionic	635:639	arg1	crossinking					641:651	rapid ionic crossinking	629:651	rapid ionic crossinking by physiologically relevant mono and divalent cations	629:705	STM-148B undergoes rapid ionic crossinking by physiologically relevant mono and divalent cations to form stable 3D hydrogels that possess excellent tissue adhesiveness, such that additional fixation aids are rendered superfluous.
32483582	1	28	theme	gellan	207:212	arg1	hydrogel					218:225	A dopamine-modified, bioinspired gellan gum hydrogel	174:225	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B)	174:236	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B) with improved physicochemical and biological characteristics, suitable for minimally invasive cell delivery and retention in the context of cartilage repair, is herein presented.
32483582	1	28	theme	gellan	207:212	arg1	STM-148B					228:235	STM-148B	228:235	STM-148B	228:235	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B) with improved physicochemical and biological characteristics, suitable for minimally invasive cell delivery and retention in the context of cartilage repair, is herein presented.
32483582	4	29	theme	chondrogenic	960:971	arg1	markers					994:1000	healthy chondrogenic extracellular matrix markers	952:1000	healthy chondrogenic extracellular matrix markers	952:1000	STM-148B hydrogels maintain viability of mammalian cells and further promote up-regulation of the expression of healthy chondrogenic extracellular matrix markers upon stimulation.
32483582	1	30	theme	gum	214:216	arg1	hydrogel					218:225	A dopamine-modified, bioinspired gellan gum hydrogel	174:225	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B)	174:236	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B) with improved physicochemical and biological characteristics, suitable for minimally invasive cell delivery and retention in the context of cartilage repair, is herein presented.
32483582	1	30	theme	gum	214:216	arg1	STM-148B					228:235	STM-148B	228:235	STM-148B	228:235	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B) with improved physicochemical and biological characteristics, suitable for minimally invasive cell delivery and retention in the context of cartilage repair, is herein presented.
32483582	0	31	theme	biological	14:23	arg1	evaluation					25:34	biological evaluation	14:34	biological evaluation	14:34	Synthesis and biological evaluation of a bioinspired, tissue-adhesive gellan gum-based hydrogel designed for minimally invasive delivery and retention of chondrogenic cells.
32483582	2	32	theme	formulation	597:607	arg1	composition					541:551	a highly biocompatible, animal-free and chemically defined composition	482:551	a highly biocompatible, animal-free and chemically defined composition	482:551	STM-148B's putative game-changing design characteristics include a highly biocompatible, animal-free and chemically defined composition, reproducibility of manufacture and ease of formulation.
32483582	2	32	theme	formulation	597:607	arg1	ease					589:592	ease	589:592	ease of formulation	589:607	STM-148B's putative game-changing design characteristics include a highly biocompatible, animal-free and chemically defined composition, reproducibility of manufacture and ease of formulation.
32483582	2	32	theme	formulation	597:607	arg1	reproducibility					554:568	reproducibility	554:568	reproducibility of manufacture	554:583	STM-148B's putative game-changing design characteristics include a highly biocompatible, animal-free and chemically defined composition, reproducibility of manufacture and ease of formulation.
32483582	3	33	theme	stable	715:720	arg1	hydrogels					725:733	stable 3D hydrogels	715:733	stable 3D hydrogels that possess excellent tissue adhesiveness, such that additional fixation aids are rendered superfluous	715:837	STM-148B undergoes rapid ionic crossinking by physiologically relevant mono and divalent cations to form stable 3D hydrogels that possess excellent tissue adhesiveness, such that additional fixation aids are rendered superfluous.
32483582	2	34	theme	game-changing	437:449	arg1	characteristics					458:472	STM-148B's putative game-changing design characteristics	417:472	STM-148B's putative game-changing design characteristics	417:472	STM-148B's putative game-changing design characteristics include a highly biocompatible, animal-free and chemically defined composition, reproducibility of manufacture and ease of formulation.
32483582	1	35	theme	invasive	323:330	arg1	delivery					337:344	minimally invasive cell delivery	313:344	minimally invasive cell delivery	313:344	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B) with improved physicochemical and biological characteristics, suitable for minimally invasive cell delivery and retention in the context of cartilage repair, is herein presented.
32483582	3	36	theme	3D	722:723	arg1	hydrogels					725:733	stable 3D hydrogels	715:733	stable 3D hydrogels that possess excellent tissue adhesiveness, such that additional fixation aids are rendered superfluous	715:837	STM-148B undergoes rapid ionic crossinking by physiologically relevant mono and divalent cations to form stable 3D hydrogels that possess excellent tissue adhesiveness, such that additional fixation aids are rendered superfluous.
32483582	3	37	contain	possess	740:746	arg2	adhesiveness					765:776	excellent tissue adhesiveness	748:776	excellent tissue adhesiveness	748:776	STM-148B undergoes rapid ionic crossinking by physiologically relevant mono and divalent cations to form stable 3D hydrogels that possess excellent tissue adhesiveness, such that additional fixation aids are rendered superfluous.
32483582	3	37	contain	possess	740:746	arg1	hydrogels					725:733	stable 3D hydrogels	715:733	stable 3D hydrogels that possess excellent tissue adhesiveness, such that additional fixation aids are rendered superfluous	715:837	STM-148B undergoes rapid ionic crossinking by physiologically relevant mono and divalent cations to form stable 3D hydrogels that possess excellent tissue adhesiveness, such that additional fixation aids are rendered superfluous.
32483582	2	38	theme	putative	428:435	arg1	characteristics					458:472	STM-148B's putative game-changing design characteristics	417:472	STM-148B's putative game-changing design characteristics	417:472	STM-148B's putative game-changing design characteristics include a highly biocompatible, animal-free and chemically defined composition, reproducibility of manufacture and ease of formulation.
32483582	2	39	theme	animal-free	506:516	arg1	composition					541:551	a highly biocompatible, animal-free and chemically defined composition	482:551	a highly biocompatible, animal-free and chemically defined composition	482:551	STM-148B's putative game-changing design characteristics include a highly biocompatible, animal-free and chemically defined composition, reproducibility of manufacture and ease of formulation.
32483582	2	39	theme	animal-free	506:516	arg1	ease					589:592	ease	589:592	ease of formulation	589:607	STM-148B's putative game-changing design characteristics include a highly biocompatible, animal-free and chemically defined composition, reproducibility of manufacture and ease of formulation.
32483582	2	39	theme	animal-free	506:516	arg1	reproducibility					554:568	reproducibility	554:568	reproducibility of manufacture	554:583	STM-148B's putative game-changing design characteristics include a highly biocompatible, animal-free and chemically defined composition, reproducibility of manufacture and ease of formulation.
32483582	1	40	theme	cell	332:335	arg1	delivery					337:344	minimally invasive cell delivery	313:344	minimally invasive cell delivery	313:344	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B) with improved physicochemical and biological characteristics, suitable for minimally invasive cell delivery and retention in the context of cartilage repair, is herein presented.
32483582	1	41	with	hydrogel	218:225	arg1	characteristics					283:297	improved physicochemical and biological characteristics	243:297	improved physicochemical and biological characteristics	243:297	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B) with improved physicochemical and biological characteristics, suitable for minimally invasive cell delivery and retention in the context of cartilage repair, is herein presented.
32483582	0	42	dep	bioinspired	41:51	arg1	tissue-adhesive					54:68	tissue-adhesive	54:68	tissue-adhesive	54:68	Synthesis and biological evaluation of a bioinspired, tissue-adhesive gellan gum-based hydrogel designed for minimally invasive delivery and retention of chondrogenic cells.
32483582	5	43	dep	cell	1121:1124	arg1	delivery					1126:1133	delivery	1126:1133	delivery	1126:1133	STM-148B is currently undergoing pre-clinical safety and efficacy assessment as a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage and may represent a valuable addition to the armamentarium of tissue-engineering therapies for treatment of focal cartilage lesions.
32483582	0	44	theme	cells	167:171	arg1	retention					141:149	retention	141:149	retention of chondrogenic cells	141:171	Synthesis and biological evaluation of a bioinspired, tissue-adhesive gellan gum-based hydrogel designed for minimally invasive delivery and retention of chondrogenic cells.
32483582	0	44	theme	cells	167:171	arg1	delivery					128:135	minimally invasive delivery	109:135	minimally invasive delivery	109:135	Synthesis and biological evaluation of a bioinspired, tissue-adhesive gellan gum-based hydrogel designed for minimally invasive delivery and retention of chondrogenic cells.
32483582	5	45	theme	valuable	1221:1228	arg1	addition					1230:1237	a valuable addition	1219:1237	a valuable addition to the armamentarium of tissue-engineering therapies for treatment of focal cartilage lesions	1219:1331	STM-148B is currently undergoing pre-clinical safety and efficacy assessment as a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage and may represent a valuable addition to the armamentarium of tissue-engineering therapies for treatment of focal cartilage lesions.
32483582	1	46	dep	dopamine-modified	176:192	arg1	bioinspired					195:205	bioinspired	195:205	bioinspired	195:205	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B) with improved physicochemical and biological characteristics, suitable for minimally invasive cell delivery and retention in the context of cartilage repair, is herein presented.
32483582	0	47	theme	bioinspired	41:51	arg1	gellan					70:75	a bioinspired, tissue-adhesive gellan	39:75	a bioinspired, tissue-adhesive gellan	39:75	Synthesis and biological evaluation of a bioinspired, tissue-adhesive gellan gum-based hydrogel designed for minimally invasive delivery and retention of chondrogenic cells.
32483582	5	48	theme	focal	1309:1313	arg1	lesions					1325:1331	focal cartilage lesions	1309:1331	focal cartilage lesions	1309:1331	STM-148B is currently undergoing pre-clinical safety and efficacy assessment as a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage and may represent a valuable addition to the armamentarium of tissue-engineering therapies for treatment of focal cartilage lesions.
32483582	0	49	theme	chondrogenic	154:165	arg1	cells					167:171	chondrogenic cells	154:171	chondrogenic cells	154:171	Synthesis and biological evaluation of a bioinspired, tissue-adhesive gellan gum-based hydrogel designed for minimally invasive delivery and retention of chondrogenic cells.
32483582	5	50	theme	hyaline-like	1178:1189	arg1	cartilage					1191:1199	hyaline-like cartilage	1178:1199	hyaline-like cartilage	1178:1199	STM-148B is currently undergoing pre-clinical safety and efficacy assessment as a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage and may represent a valuable addition to the armamentarium of tissue-engineering therapies for treatment of focal cartilage lesions.
32483582	5	51	theme	cartilage	1315:1323	arg1	lesions					1325:1331	focal cartilage lesions	1309:1331	focal cartilage lesions	1309:1331	STM-148B is currently undergoing pre-clinical safety and efficacy assessment as a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage and may represent a valuable addition to the armamentarium of tissue-engineering therapies for treatment of focal cartilage lesions.
32483582	3	52	theme	excellent	748:756	arg1	adhesiveness					765:776	excellent tissue adhesiveness	748:776	excellent tissue adhesiveness	748:776	STM-148B undergoes rapid ionic crossinking by physiologically relevant mono and divalent cations to form stable 3D hydrogels that possess excellent tissue adhesiveness, such that additional fixation aids are rendered superfluous.
32483582	3	53	theme	divalent	690:697	arg1	cations					699:705	physiologically relevant mono and divalent cations	656:705	cations	699:705	STM-148B undergoes rapid ionic crossinking by physiologically relevant mono and divalent cations to form stable 3D hydrogels that possess excellent tissue adhesiveness, such that additional fixation aids are rendered superfluous.
32483582	3	54	theme	relevant	672:679	arg1	mono					681:684	physiologically relevant mono and divalent cations	656:705	mono	681:684	STM-148B undergoes rapid ionic crossinking by physiologically relevant mono and divalent cations to form stable 3D hydrogels that possess excellent tissue adhesiveness, such that additional fixation aids are rendered superfluous.
32483582	5	55	theme	efficacy	1077:1084	arg1	safety					1066:1071	pre-clinical safety and efficacy assessment	1053:1095	safety	1066:1071	STM-148B is currently undergoing pre-clinical safety and efficacy assessment as a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage and may represent a valuable addition to the armamentarium of tissue-engineering therapies for treatment of focal cartilage lesions.
32483582	5	55	theme	efficacy	1077:1084	arg1	assessment					1086:1095	pre-clinical safety and efficacy assessment	1053:1095	assessment	1086:1095	STM-148B is currently undergoing pre-clinical safety and efficacy assessment as a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage and may represent a valuable addition to the armamentarium of tissue-engineering therapies for treatment of focal cartilage lesions.
32483582	5	55	theme	efficacy	1077:1084	arg1	device					1110:1115	a medical device	1100:1115	a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage	1100:1199	STM-148B is currently undergoing pre-clinical safety and efficacy assessment as a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage and may represent a valuable addition to the armamentarium of tissue-engineering therapies for treatment of focal cartilage lesions.
32483582	5	56	theme	cartilage	1191:1199	arg1	regeneration					1162:1173	regeneration	1162:1173	regeneration of hyaline-like cartilage	1162:1199	STM-148B is currently undergoing pre-clinical safety and efficacy assessment as a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage and may represent a valuable addition to the armamentarium of tissue-engineering therapies for treatment of focal cartilage lesions.
32483582	5	57	theme	pre-clinical	1053:1064	arg1	safety					1066:1071	pre-clinical safety and efficacy assessment	1053:1095	safety	1066:1071	STM-148B is currently undergoing pre-clinical safety and efficacy assessment as a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage and may represent a valuable addition to the armamentarium of tissue-engineering therapies for treatment of focal cartilage lesions.
32483582	5	57	theme	pre-clinical	1053:1064	arg1	assessment					1086:1095	pre-clinical safety and efficacy assessment	1053:1095	assessment	1086:1095	STM-148B is currently undergoing pre-clinical safety and efficacy assessment as a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage and may represent a valuable addition to the armamentarium of tissue-engineering therapies for treatment of focal cartilage lesions.
32483582	5	57	theme	pre-clinical	1053:1064	arg1	device					1110:1115	a medical device	1100:1115	a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage	1100:1199	STM-148B is currently undergoing pre-clinical safety and efficacy assessment as a medical device for cell delivery and retention focussing on regeneration of hyaline-like cartilage and may represent a valuable addition to the armamentarium of tissue-engineering therapies for treatment of focal cartilage lesions.
32483582	4	58	theme	mammalian	881:889	arg1	cells					891:895	mammalian cells	881:895	mammalian cells	881:895	STM-148B hydrogels maintain viability of mammalian cells and further promote up-regulation of the expression of healthy chondrogenic extracellular matrix markers upon stimulation.
32483582	0	59	theme	gellan	70:75	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and biological evaluation of a bioinspired, tissue-adhesive gellan gum-based hydrogel designed for minimally invasive delivery and retention of chondrogenic cells.
32483582	0	59	theme	gellan	70:75	arg1	evaluation					25:34	biological evaluation	14:34	biological evaluation	14:34	Synthesis and biological evaluation of a bioinspired, tissue-adhesive gellan gum-based hydrogel designed for minimally invasive delivery and retention of chondrogenic cells.
32483582	2	60	theme	design	451:456	arg1	characteristics					458:472	STM-148B's putative game-changing design characteristics	417:472	STM-148B's putative game-changing design characteristics	417:472	STM-148B's putative game-changing design characteristics include a highly biocompatible, animal-free and chemically defined composition, reproducibility of manufacture and ease of formulation.
32483582	1	61	theme	improved	243:250	arg1	characteristics					283:297	improved physicochemical and biological characteristics	243:297	improved physicochemical and biological characteristics	243:297	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B) with improved physicochemical and biological characteristics, suitable for minimally invasive cell delivery and retention in the context of cartilage repair, is herein presented.
32483582	4	62	theme	cells	891:895	arg1	viability					868:876	viability	868:876	viability of mammalian cells	868:895	STM-148B hydrogels maintain viability of mammalian cells and further promote up-regulation of the expression of healthy chondrogenic extracellular matrix markers upon stimulation.
32483582	1	63	theme	physicochemical	252:266	arg1	characteristics					283:297	improved physicochemical and biological characteristics	243:297	improved physicochemical and biological characteristics	243:297	A dopamine-modified, bioinspired gellan gum hydrogel (STM-148B) with improved physicochemical and biological characteristics, suitable for minimally invasive cell delivery and retention in the context of cartilage repair, is herein presented.
31944086	8	0	theme	aroma	1506:1510	arg1	compounds					1512:1520	all aroma compounds	1502:1520	all aroma compounds	1502:1520	Increasing the concentration of GA provides the highest retention for all aroma compounds and improves the viscosity and the emulsion stability of spray-dried powder.
31944086	2	1	theme	traditional	336:346	arg1	caseinate					418:426	sodium caseinate	411:426	sodium caseinate (SC)	411:431	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	2	1	theme	traditional	336:346	arg1	maltodextrin					389:400	maltodextrin	389:400	maltodextrin (MD)	389:405	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	2	1	theme	traditional	336:346	arg1	arabic					376:381	gum arabic	372:381	gum arabic (GA)	372:386	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	2	1	theme	traditional	336:346	arg1	materials					353:361	traditional wall materials	336:361	traditional wall materials	336:361	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	6	2	from	effect	1117:1122	arg1	retention					1151:1159	flavor retention	1144:1159	flavor retention	1144:1159	The effect of encapsulation on flavor retention and chemical composition was evaluated using gas chromatography-mass spectrometry (GC-MS).
31944086	6	2	from	effect	1117:1122	arg1	composition					1174:1184	chemical composition	1165:1184	chemical composition	1165:1184	The effect of encapsulation on flavor retention and chemical composition was evaluated using gas chromatography-mass spectrometry (GC-MS).
31944086	11	3	theme	nutraceutical	2084:2096	arg1	premixes					2105:2112	nutraceutical powder premixes	2084:2112	nutraceutical powder premixes	2084:2112	CONCLUSION Wall material formulation affects the retention, morphology and physical properties of the encapsulated flavors, which can be used in food or nutraceutical powder premixes.
31944086	10	4	theme	encapsulated	1807:1818	arg1	samples					1820:1826	encapsulated samples	1807:1826	encapsulated samples containing citral and orange oils	1807:1860	The NEB is observed intensively in encapsulated samples containing citral and orange oils, whereas the least browning occurs in isoamyl acetate microcapsules.
31944086	6	5	theme	flavor	1144:1149	arg1	retention					1151:1159	flavor retention	1144:1159	flavor retention	1144:1159	The effect of encapsulation on flavor retention and chemical composition was evaluated using gas chromatography-mass spectrometry (GC-MS).
31944086	8	6	theme	powder	1591:1596	arg1	viscosity					1539:1547	the viscosity	1535:1547	the viscosity	1535:1547	Increasing the concentration of GA provides the highest retention for all aroma compounds and improves the viscosity and the emulsion stability of spray-dried powder.
31944086	8	6	theme	powder	1591:1596	arg1	stability					1566:1574	the emulsion stability	1553:1574	the emulsion stability of spray-dried powder	1553:1596	Increasing the concentration of GA provides the highest retention for all aroma compounds and improves the viscosity and the emulsion stability of spray-dried powder.
31944086	11	7	dep	retention	1980:1988	arg1	the					1976:1978	the	1976:1978	the	1976:1978	CONCLUSION Wall material formulation affects the retention, morphology and physical properties of the encapsulated flavors, which can be used in food or nutraceutical powder premixes.
31944086	6	8	theme	encapsulation	1127:1139	arg1	effect					1117:1122	The effect	1113:1122	The effect of encapsulation on flavor retention and chemical composition	1113:1184	The effect of encapsulation on flavor retention and chemical composition was evaluated using gas chromatography-mass spectrometry (GC-MS).
31944086	4	9	theme	spray	922:926	arg1	process					935:941	the spray drying process	918:941	the spray drying process	918:941	Physical properties, including encapsulation efficiency, viscosity, emulsion stability and moisture, were studied before and after the spray drying process.
31944086	10	10	located	observed	1783:1790	arg1	samples					1820:1826	encapsulated samples	1807:1826	encapsulated samples containing citral and orange oils	1807:1860	The NEB is observed intensively in encapsulated samples containing citral and orange oils, whereas the least browning occurs in isoamyl acetate microcapsules.
31944086	10	10	located	observed	1783:1790	arg2	NEB					1776:1778	The NEB	1772:1778	The NEB	1772:1778	The NEB is observed intensively in encapsulated samples containing citral and orange oils, whereas the least browning occurs in isoamyl acetate microcapsules.
31944086	7	11	theme	isoamyl	1332:1338	arg1	acetate					1340:1346	isoamyl acetate	1332:1346	isoamyl acetate	1332:1346	RESULTS Orange oil shows the maximum retention efficiency (84.5-97.9%), whereas isoamyl acetate is less retained during drying (44.4-72.5%) compared with other volatile compounds.
31944086	11	12	theme	Wall	1942:1945	arg1	formulation					1956:1966	CONCLUSION Wall material formulation	1931:1966	CONCLUSION Wall material formulation	1931:1966	CONCLUSION Wall material formulation affects the retention, morphology and physical properties of the encapsulated flavors, which can be used in food or nutraceutical powder premixes.
31944086	7	13	theme	maximum	1281:1287	arg1	efficiency					1299:1308	the maximum retention efficiency	1277:1308	the maximum retention efficiency (84.5-97.9%)	1277:1321	RESULTS Orange oil shows the maximum retention efficiency (84.5-97.9%), whereas isoamyl acetate is less retained during drying (44.4-72.5%) compared with other volatile compounds.
31944086	7	13	theme	maximum	1281:1287	arg1	%					1320:1320	84.5-97.9%	1311:1320	84.5-97.9%	1311:1320	RESULTS Orange oil shows the maximum retention efficiency (84.5-97.9%), whereas isoamyl acetate is less retained during drying (44.4-72.5%) compared with other volatile compounds.
31944086	2	14	theme	materials	476:484	arg1	effects					450:456	the effects	446:456	the effects of different wall materials on the properties of the flavor microcapsules	446:530	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	6	15	theme	gas	1206:1208	arg1	GC-MS					1244:1248	GC-MS	1244:1248	GC-MS	1244:1248	The effect of encapsulation on flavor retention and chemical composition was evaluated using gas chromatography-mass spectrometry (GC-MS).
31944086	6	15	theme	gas	1206:1208	arg1	spectrometry					1230:1241	gas chromatography-mass spectrometry	1206:1241	gas chromatography-mass spectrometry (GC-MS)	1206:1249	The effect of encapsulation on flavor retention and chemical composition was evaluated using gas chromatography-mass spectrometry (GC-MS).
31944086	11	16	theme	flavors	2046:2052	arg1	morphology					1991:2000	morphology	1991:2000	morphology	1991:2000	CONCLUSION Wall material formulation affects the retention, morphology and physical properties of the encapsulated flavors, which can be used in food or nutraceutical powder premixes.
31944086	11	16	theme	flavors	2046:2052	arg1	properties					2015:2024	physical properties	2006:2024	physical properties	2006:2024	CONCLUSION Wall material formulation affects the retention, morphology and physical properties of the encapsulated flavors, which can be used in food or nutraceutical powder premixes.
31944086	11	16	theme	flavors	2046:2052	arg1	retention					1980:1988	retention	1980:1988	retention	1980:1988	CONCLUSION Wall material formulation affects the retention, morphology and physical properties of the encapsulated flavors, which can be used in food or nutraceutical powder premixes.
31944086	7	17	dep	RESULTS	1252:1258	arg1	shows					1271:1275	shows	1271:1275	shows	1271:1275	RESULTS Orange oil shows the maximum retention efficiency (84.5-97.9%), whereas isoamyl acetate is less retained during drying (44.4-72.5%) compared with other volatile compounds.
31944086	2	18	theme	flavor	511:516	arg1	microcapsules					518:530	the flavor microcapsules	507:530	the flavor microcapsules	507:530	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	1	19	theme	chemical	259:266	arg1	interactions					268:279	chemical interactions	259:279	chemical interactions	259:279	BACKGROUND AND OBJECTIVE Encapsulation is an effective method to keep the quality of and avoid changes in flavors or essential oils due to oxidation, heating, volatilization, or chemical interactions.
31944086	7	20	theme	Orange	1260:1265	arg1	oil					1267:1269	Orange oil	1260:1269	Orange oil	1260:1269	RESULTS Orange oil shows the maximum retention efficiency (84.5-97.9%), whereas isoamyl acetate is less retained during drying (44.4-72.5%) compared with other volatile compounds.
31944086	10	21	contain	containing	1828:1837	arg1	samples					1820:1826	encapsulated samples	1807:1826	encapsulated samples containing citral and orange oils	1807:1860	The NEB is observed intensively in encapsulated samples containing citral and orange oils, whereas the least browning occurs in isoamyl acetate microcapsules.
31944086	10	21	contain	containing	1828:1837	arg2	oils					1857:1860	citral and orange oils	1839:1860	citral and orange oils	1839:1860	The NEB is observed intensively in encapsulated samples containing citral and orange oils, whereas the least browning occurs in isoamyl acetate microcapsules.
31944086	1	22	theme	effective	126:134	arg1	method					136:141	an effective method	123:141	an effective method to keep the quality of and avoid changes in flavors or essential oils due to oxidation, heating, volatilization, or chemical interactions	123:279	BACKGROUND AND OBJECTIVE Encapsulation is an effective method to keep the quality of and avoid changes in flavors or essential oils due to oxidation, heating, volatilization, or chemical interactions.
31944086	1	22	theme	effective	126:134	arg1	OBJECTIVE					96:104	OBJECTIVE	96:104	OBJECTIVE	96:104	BACKGROUND AND OBJECTIVE Encapsulation is an effective method to keep the quality of and avoid changes in flavors or essential oils due to oxidation, heating, volatilization, or chemical interactions.
31944086	1	22	theme	effective	126:134	arg1	BACKGROUND					81:90	BACKGROUND	81:90	BACKGROUND	81:90	BACKGROUND AND OBJECTIVE Encapsulation is an effective method to keep the quality of and avoid changes in flavors or essential oils due to oxidation, heating, volatilization, or chemical interactions.
31944086	9	23	theme	MD	1615:1616	arg1	presence					1603:1610	The presence	1599:1610	The presence of MD as the main encapsulating agent	1599:1648	The presence of MD as the main encapsulating agent enables the formation of several homogeneous capsules with a good spherical shape and a smooth surface, according to SEM.
31944086	1	24	from	changes	176:182	arg1	flavors					187:193	flavors	187:193	flavors	187:193	BACKGROUND AND OBJECTIVE Encapsulation is an effective method to keep the quality of and avoid changes in flavors or essential oils due to oxidation, heating, volatilization, or chemical interactions.
31944086	1	24	from	changes	176:182	arg1	oils					208:211	essential oils	198:211	essential oils	198:211	BACKGROUND AND OBJECTIVE Encapsulation is an effective method to keep the quality of and avoid changes in flavors or essential oils due to oxidation, heating, volatilization, or chemical interactions.
31944086	11	25	theme	powder	2098:2103	arg1	premixes					2105:2112	nutraceutical powder premixes	2084:2112	nutraceutical powder premixes	2084:2112	CONCLUSION Wall material formulation affects the retention, morphology and physical properties of the encapsulated flavors, which can be used in food or nutraceutical powder premixes.
31944086	10	26	theme	least	1875:1879	arg1	browning					1881:1888	the least browning	1871:1888	the least browning	1871:1888	The NEB is observed intensively in encapsulated samples containing citral and orange oils, whereas the least browning occurs in isoamyl acetate microcapsules.
31944086	0	27	theme	Functional	53:62	arg1	Characteristics					64:78	Their Functional Characteristics	47:78	Their Functional Characteristics	47:78	Effects of Carriers on Spray-dried Flavors and Their Functional Characteristics.
31944086	9	28	theme	encapsulating	1630:1642	arg1	agent					1644:1648	the main encapsulating agent	1621:1648	the main encapsulating agent	1621:1648	The presence of MD as the main encapsulating agent enables the formation of several homogeneous capsules with a good spherical shape and a smooth surface, according to SEM.
31944086	5	29	theme	powder	1018:1023	arg1	particles					1025:1033	powder particles	1018:1033	powder particles	1018:1033	Moreover, the morphology and extent of the nonenzymatic browning (NEB) of powder particles were performed using a scanning electron microscope (SEM) and a chroma meter.
31944086	2	30	theme	different	461:469	arg1	materials					476:484	different wall materials	461:484	different wall materials	461:484	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	0	31	from	Effects	0:6	arg1	Flavors					35:41	Spray-dried Flavors	23:41	Spray-dried Flavors	23:41	Effects of Carriers on Spray-dried Flavors and Their Functional Characteristics.
31944086	0	31	from	Effects	0:6	arg1	Characteristics					64:78	Their Functional Characteristics	47:78	Their Functional Characteristics	47:78	Effects of Carriers on Spray-dried Flavors and Their Functional Characteristics.
31944086	10	32	theme	isoamyl	1900:1906	arg1	microcapsules					1916:1928	isoamyl acetate microcapsules	1900:1928	isoamyl acetate microcapsules	1900:1928	The NEB is observed intensively in encapsulated samples containing citral and orange oils, whereas the least browning occurs in isoamyl acetate microcapsules.
31944086	5	33	theme	nonenzymatic	987:998	arg1	NEB					1010:1012	NEB	1010:1012	NEB	1010:1012	Moreover, the morphology and extent of the nonenzymatic browning (NEB) of powder particles were performed using a scanning electron microscope (SEM) and a chroma meter.
31944086	5	33	theme	nonenzymatic	987:998	arg1	browning					1000:1007	the nonenzymatic browning	983:1007	the nonenzymatic browning (NEB) of powder particles	983:1033	Moreover, the morphology and extent of the nonenzymatic browning (NEB) of powder particles were performed using a scanning electron microscope (SEM) and a chroma meter.
31944086	9	34	theme	several	1675:1681	arg1	capsules					1695:1702	several homogeneous capsules	1675:1702	several homogeneous capsules with a good spherical shape and a smooth surface, according to SEM	1675:1769	The presence of MD as the main encapsulating agent enables the formation of several homogeneous capsules with a good spherical shape and a smooth surface, according to SEM.
31944086	5	35	theme	scanning	1058:1065	arg1	SEM					1088:1090	SEM	1088:1090	SEM	1088:1090	Moreover, the morphology and extent of the nonenzymatic browning (NEB) of powder particles were performed using a scanning electron microscope (SEM) and a chroma meter.
31944086	5	35	theme	scanning	1058:1065	arg1	microscope					1076:1085	a scanning electron microscope	1056:1085	a scanning electron microscope (SEM)	1056:1091	Moreover, the morphology and extent of the nonenzymatic browning (NEB) of powder particles were performed using a scanning electron microscope (SEM) and a chroma meter.
31944086	9	36	theme	capsules	1695:1702	arg1	formation					1662:1670	the formation	1658:1670	the formation of several homogeneous capsules with a good spherical shape and a smooth surface, according to SEM	1658:1769	The presence of MD as the main encapsulating agent enables the formation of several homogeneous capsules with a good spherical shape and a smooth surface, according to SEM.
31944086	10	37	dep	observed	1783:1790	arg1	whereas					1863:1869	whereas	1863:1869	whereas	1863:1869	The NEB is observed intensively in encapsulated samples containing citral and orange oils, whereas the least browning occurs in isoamyl acetate microcapsules.
31944086	0	38	theme	Carriers	11:18	arg1	Effects					0:6	Effects	0:6	Effects of Carriers on Spray-dried Flavors and Their Functional Characteristics	0:78	Effects of Carriers on Spray-dried Flavors and Their Functional Characteristics.
31944086	7	39	theme	other	1406:1410	arg1	compounds					1421:1429	other volatile compounds	1406:1429	other volatile compounds	1406:1429	RESULTS Orange oil shows the maximum retention efficiency (84.5-97.9%), whereas isoamyl acetate is less retained during drying (44.4-72.5%) compared with other volatile compounds.
31944086	0	40	theme	Spray-dried	23:33	arg1	Flavors					35:41	Spray-dried Flavors	23:41	Spray-dried Flavors	23:41	Effects of Carriers on Spray-dried Flavors and Their Functional Characteristics.
31944086	9	41	theme	spherical	1716:1724	arg1	shape					1726:1730	a good spherical shape	1709:1730	a good spherical shape	1709:1730	The presence of MD as the main encapsulating agent enables the formation of several homogeneous capsules with a good spherical shape and a smooth surface, according to SEM.
31944086	3	42	theme	compounds	579:587	arg1	emulsions					559:567	The emulsions	555:567	The emulsions of flavor compounds	555:587	MATERIALS AND METHODS The emulsions of flavor compounds (linalool, citral, orange oil, allyl caproate and isoamyl acetate) were prepared using GA, SC and MD as carriers with different concentrations and then encapsulated in powder form by a spray dryer.
31944086	8	43	theme	GA	1464:1465	arg1	concentration					1447:1459	the concentration	1443:1459	the concentration of GA	1443:1465	Increasing the concentration of GA provides the highest retention for all aroma compounds and improves the viscosity and the emulsion stability of spray-dried powder.
31944086	3	44	theme	orange	608:613	arg1	MATERIALS					533:541	MATERIALS	533:541	MATERIALS	533:541	MATERIALS AND METHODS The emulsions of flavor compounds (linalool, citral, orange oil, allyl caproate and isoamyl acetate) were prepared using GA, SC and MD as carriers with different concentrations and then encapsulated in powder form by a spray dryer.
31944086	3	44	theme	orange	608:613	arg1	oil					615:617	orange oil	608:617	orange oil	608:617	MATERIALS AND METHODS The emulsions of flavor compounds (linalool, citral, orange oil, allyl caproate and isoamyl acetate) were prepared using GA, SC and MD as carriers with different concentrations and then encapsulated in powder form by a spray dryer.
31944086	2	45	theme	key	318:320	arg1	flavorings					322:331	key flavorings	318:331	key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC)	318:431	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	1	46	theme	due	213:215	arg1	flavors					187:193	flavors	187:193	flavors	187:193	BACKGROUND AND OBJECTIVE Encapsulation is an effective method to keep the quality of and avoid changes in flavors or essential oils due to oxidation, heating, volatilization, or chemical interactions.
31944086	9	47	theme	smooth	1738:1743	arg1	surface					1745:1751	a smooth surface	1736:1751	a smooth surface	1736:1751	The presence of MD as the main encapsulating agent enables the formation of several homogeneous capsules with a good spherical shape and a smooth surface, according to SEM.
31944086	2	48	theme	sodium	411:416	arg1	materials					353:361	traditional wall materials	336:361	traditional wall materials	336:361	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	2	48	theme	sodium	411:416	arg1	SC					429:430	SC	429:430	SC	429:430	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	2	48	theme	sodium	411:416	arg1	caseinate					418:426	sodium caseinate	411:426	sodium caseinate (SC)	411:431	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	6	49	theme	chemical	1165:1172	arg1	composition					1174:1184	chemical composition	1165:1184	chemical composition	1165:1184	The effect of encapsulation on flavor retention and chemical composition was evaluated using gas chromatography-mass spectrometry (GC-MS).
31944086	2	50	theme	wall	348:351	arg1	caseinate					418:426	sodium caseinate	411:426	sodium caseinate (SC)	411:431	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	2	50	theme	wall	348:351	arg1	maltodextrin					389:400	maltodextrin	389:400	maltodextrin (MD)	389:405	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	2	50	theme	wall	348:351	arg1	arabic					376:381	gum arabic	372:381	gum arabic (GA)	372:386	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	2	50	theme	wall	348:351	arg1	materials					353:361	traditional wall materials	336:361	traditional wall materials	336:361	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	11	51	theme	physical	2006:2013	arg1	properties					2015:2024	physical properties	2006:2024	physical properties	2006:2024	CONCLUSION Wall material formulation affects the retention, morphology and physical properties of the encapsulated flavors, which can be used in food or nutraceutical powder premixes.
31944086	5	52	dep	morphology	958:967	arg1	the					954:956	the	954:956	the	954:956	Moreover, the morphology and extent of the nonenzymatic browning (NEB) of powder particles were performed using a scanning electron microscope (SEM) and a chroma meter.
31944086	2	53	theme	microcapsules	518:530	arg1	properties					493:502	the properties	489:502	the properties of the flavor microcapsules	489:530	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	8	54	theme	spray-dried	1579:1589	arg1	powder					1591:1596	spray-dried powder	1579:1596	spray-dried powder	1579:1596	Increasing the concentration of GA provides the highest retention for all aroma compounds and improves the viscosity and the emulsion stability of spray-dried powder.
31944086	11	55	theme	encapsulated	2033:2044	arg1	flavors					2046:2052	the encapsulated flavors	2029:2052	the encapsulated flavors	2029:2052	CONCLUSION Wall material formulation affects the retention, morphology and physical properties of the encapsulated flavors, which can be used in food or nutraceutical powder premixes.
31944086	3	56	theme	isoamyl	639:645	arg1	MATERIALS					533:541	MATERIALS	533:541	MATERIALS	533:541	MATERIALS AND METHODS The emulsions of flavor compounds (linalool, citral, orange oil, allyl caproate and isoamyl acetate) were prepared using GA, SC and MD as carriers with different concentrations and then encapsulated in powder form by a spray dryer.
31944086	3	56	theme	isoamyl	639:645	arg1	acetate					647:653	isoamyl acetate	639:653	isoamyl acetate	639:653	MATERIALS AND METHODS The emulsions of flavor compounds (linalool, citral, orange oil, allyl caproate and isoamyl acetate) were prepared using GA, SC and MD as carriers with different concentrations and then encapsulated in powder form by a spray dryer.
31944086	9	57	with	capsules	1695:1702	arg1	surface					1745:1751	a smooth surface	1736:1751	a smooth surface	1736:1751	The presence of MD as the main encapsulating agent enables the formation of several homogeneous capsules with a good spherical shape and a smooth surface, according to SEM.
31944086	9	57	with	capsules	1695:1702	arg1	shape					1726:1730	a good spherical shape	1709:1730	a good spherical shape	1709:1730	The presence of MD as the main encapsulating agent enables the formation of several homogeneous capsules with a good spherical shape and a smooth surface, according to SEM.
31944086	3	58	dep	MATERIALS	533:541	arg1	MATERIALS					533:541	MATERIALS	533:541	MATERIALS	533:541	MATERIALS AND METHODS The emulsions of flavor compounds (linalool, citral, orange oil, allyl caproate and isoamyl acetate) were prepared using GA, SC and MD as carriers with different concentrations and then encapsulated in powder form by a spray dryer.
31944086	3	58	dep	MATERIALS	533:541	arg1	acetate					647:653	isoamyl acetate	639:653	isoamyl acetate	639:653	MATERIALS AND METHODS The emulsions of flavor compounds (linalool, citral, orange oil, allyl caproate and isoamyl acetate) were prepared using GA, SC and MD as carriers with different concentrations and then encapsulated in powder form by a spray dryer.
31944086	3	58	dep	MATERIALS	533:541	arg1	oil					615:617	orange oil	608:617	orange oil	608:617	MATERIALS AND METHODS The emulsions of flavor compounds (linalool, citral, orange oil, allyl caproate and isoamyl acetate) were prepared using GA, SC and MD as carriers with different concentrations and then encapsulated in powder form by a spray dryer.
31944086	3	58	dep	MATERIALS	533:541	arg1	linalool					590:597	linalool	590:597	linalool	590:597	MATERIALS AND METHODS The emulsions of flavor compounds (linalool, citral, orange oil, allyl caproate and isoamyl acetate) were prepared using GA, SC and MD as carriers with different concentrations and then encapsulated in powder form by a spray dryer.
31944086	3	58	dep	MATERIALS	533:541	arg1	caproate					626:633	allyl caproate	620:633	allyl caproate	620:633	MATERIALS AND METHODS The emulsions of flavor compounds (linalool, citral, orange oil, allyl caproate and isoamyl acetate) were prepared using GA, SC and MD as carriers with different concentrations and then encapsulated in powder form by a spray dryer.
31944086	3	58	dep	MATERIALS	533:541	arg1	emulsions					559:567	The emulsions	555:567	The emulsions of flavor compounds	555:587	MATERIALS AND METHODS The emulsions of flavor compounds (linalool, citral, orange oil, allyl caproate and isoamyl acetate) were prepared using GA, SC and MD as carriers with different concentrations and then encapsulated in powder form by a spray dryer.
31944086	3	58	dep	MATERIALS	533:541	arg1	citral					600:605	citral	600:605	citral	600:605	MATERIALS AND METHODS The emulsions of flavor compounds (linalool, citral, orange oil, allyl caproate and isoamyl acetate) were prepared using GA, SC and MD as carriers with different concentrations and then encapsulated in powder form by a spray dryer.
31944086	10	59	theme	citral	1839:1844	arg1	oils					1857:1860	citral and orange oils	1839:1860	citral and orange oils	1839:1860	The NEB is observed intensively in encapsulated samples containing citral and orange oils, whereas the least browning occurs in isoamyl acetate microcapsules.
31944086	11	60	theme	material	1947:1954	arg1	formulation					1956:1966	CONCLUSION Wall material formulation	1931:1966	CONCLUSION Wall material formulation	1931:1966	CONCLUSION Wall material formulation affects the retention, morphology and physical properties of the encapsulated flavors, which can be used in food or nutraceutical powder premixes.
31944086	7	61	theme	retention	1289:1297	arg1	efficiency					1299:1308	the maximum retention efficiency	1277:1308	the maximum retention efficiency (84.5-97.9%)	1277:1321	RESULTS Orange oil shows the maximum retention efficiency (84.5-97.9%), whereas isoamyl acetate is less retained during drying (44.4-72.5%) compared with other volatile compounds.
31944086	7	61	theme	retention	1289:1297	arg1	%					1320:1320	84.5-97.9%	1311:1320	84.5-97.9%	1311:1320	RESULTS Orange oil shows the maximum retention efficiency (84.5-97.9%), whereas isoamyl acetate is less retained during drying (44.4-72.5%) compared with other volatile compounds.
31944086	6	62	theme	chromatography-mass	1210:1228	arg1	GC-MS					1244:1248	GC-MS	1244:1248	GC-MS	1244:1248	The effect of encapsulation on flavor retention and chemical composition was evaluated using gas chromatography-mass spectrometry (GC-MS).
31944086	6	62	theme	chromatography-mass	1210:1228	arg1	spectrometry					1230:1241	gas chromatography-mass spectrometry	1206:1241	gas chromatography-mass spectrometry (GC-MS)	1206:1249	The effect of encapsulation on flavor retention and chemical composition was evaluated using gas chromatography-mass spectrometry (GC-MS).
31944086	1	63	theme	essential	198:206	arg1	oils					208:211	essential oils	198:211	essential oils	198:211	BACKGROUND AND OBJECTIVE Encapsulation is an effective method to keep the quality of and avoid changes in flavors or essential oils due to oxidation, heating, volatilization, or chemical interactions.
31944086	11	64	theme	CONCLUSION	1931:1940	arg1	formulation					1956:1966	CONCLUSION Wall material formulation	1931:1966	CONCLUSION Wall material formulation	1931:1966	CONCLUSION Wall material formulation affects the retention, morphology and physical properties of the encapsulated flavors, which can be used in food or nutraceutical powder premixes.
31944086	4	65	theme	emulsion	855:862	arg1	stability					864:872	emulsion stability	855:872	emulsion stability	855:872	Physical properties, including encapsulation efficiency, viscosity, emulsion stability and moisture, were studied before and after the spray drying process.
31944086	4	66	theme	Physical	787:794	arg1	stability					864:872	emulsion stability	855:872	emulsion stability	855:872	Physical properties, including encapsulation efficiency, viscosity, emulsion stability and moisture, were studied before and after the spray drying process.
31944086	4	66	theme	Physical	787:794	arg1	efficiency					832:841	encapsulation efficiency	818:841	encapsulation efficiency	818:841	Physical properties, including encapsulation efficiency, viscosity, emulsion stability and moisture, were studied before and after the spray drying process.
31944086	4	66	theme	Physical	787:794	arg1	properties					796:805	Physical properties	787:805	Physical properties	787:805	Physical properties, including encapsulation efficiency, viscosity, emulsion stability and moisture, were studied before and after the spray drying process.
31944086	4	66	theme	Physical	787:794	arg1	moisture					878:885	moisture	878:885	moisture	878:885	Physical properties, including encapsulation efficiency, viscosity, emulsion stability and moisture, were studied before and after the spray drying process.
31944086	4	66	theme	Physical	787:794	arg1	viscosity					844:852	viscosity	844:852	viscosity	844:852	Physical properties, including encapsulation efficiency, viscosity, emulsion stability and moisture, were studied before and after the spray drying process.
31944086	2	67	from	effects	450:456	arg1	properties					493:502	the properties	489:502	the properties of the flavor microcapsules	489:530	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	1	68	dep	BACKGROUND	81:90	arg1	Encapsulation					106:118	Encapsulation	106:118	Encapsulation	106:118	BACKGROUND AND OBJECTIVE Encapsulation is an effective method to keep the quality of and avoid changes in flavors or essential oils due to oxidation, heating, volatilization, or chemical interactions.
31944086	10	69	theme	orange	1850:1855	arg1	oils					1857:1860	citral and orange oils	1839:1860	citral and orange oils	1839:1860	The NEB is observed intensively in encapsulated samples containing citral and orange oils, whereas the least browning occurs in isoamyl acetate microcapsules.
31944086	3	70	theme	different	707:715	arg1	concentrations					717:730	different concentrations	707:730	different concentrations	707:730	MATERIALS AND METHODS The emulsions of flavor compounds (linalool, citral, orange oil, allyl caproate and isoamyl acetate) were prepared using GA, SC and MD as carriers with different concentrations and then encapsulated in powder form by a spray dryer.
31944086	3	71	with	carriers	693:700	arg1	concentrations					717:730	different concentrations	707:730	different concentrations	707:730	MATERIALS AND METHODS The emulsions of flavor compounds (linalool, citral, orange oil, allyl caproate and isoamyl acetate) were prepared using GA, SC and MD as carriers with different concentrations and then encapsulated in powder form by a spray dryer.
31944086	3	72	theme	powder	757:762	arg1	form					764:767	powder form	757:767	powder form	757:767	MATERIALS AND METHODS The emulsions of flavor compounds (linalool, citral, orange oil, allyl caproate and isoamyl acetate) were prepared using GA, SC and MD as carriers with different concentrations and then encapsulated in powder form by a spray dryer.
31944086	9	73	theme	main	1625:1628	arg1	agent					1644:1648	the main encapsulating agent	1621:1648	the main encapsulating agent	1621:1648	The presence of MD as the main encapsulating agent enables the formation of several homogeneous capsules with a good spherical shape and a smooth surface, according to SEM.
31944086	5	74	theme	chroma	1099:1104	arg1	meter					1106:1110	a chroma meter	1097:1110	a chroma meter	1097:1110	Moreover, the morphology and extent of the nonenzymatic browning (NEB) of powder particles were performed using a scanning electron microscope (SEM) and a chroma meter.
31944086	4	75	theme	encapsulation	818:830	arg1	efficiency					832:841	encapsulation efficiency	818:841	encapsulation efficiency	818:841	Physical properties, including encapsulation efficiency, viscosity, emulsion stability and moisture, were studied before and after the spray drying process.
31944086	10	76	theme	acetate	1908:1914	arg1	microcapsules					1916:1928	isoamyl acetate microcapsules	1900:1928	isoamyl acetate microcapsules	1900:1928	The NEB is observed intensively in encapsulated samples containing citral and orange oils, whereas the least browning occurs in isoamyl acetate microcapsules.
31944086	5	77	theme	particles	1025:1033	arg1	NEB					1010:1012	NEB	1010:1012	NEB	1010:1012	Moreover, the morphology and extent of the nonenzymatic browning (NEB) of powder particles were performed using a scanning electron microscope (SEM) and a chroma meter.
31944086	5	77	theme	particles	1025:1033	arg1	browning					1000:1007	the nonenzymatic browning	983:1007	the nonenzymatic browning (NEB) of powder particles	983:1033	Moreover, the morphology and extent of the nonenzymatic browning (NEB) of powder particles were performed using a scanning electron microscope (SEM) and a chroma meter.
31944086	3	78	theme	spray	774:778	arg1	dryer					780:784	a spray dryer	772:784	a spray dryer	772:784	MATERIALS AND METHODS The emulsions of flavor compounds (linalool, citral, orange oil, allyl caproate and isoamyl acetate) were prepared using GA, SC and MD as carriers with different concentrations and then encapsulated in powder form by a spray dryer.
31944086	11	79	used	used	2068:2071	arg2	flavors					2046:2052	the encapsulated flavors	2029:2052	the encapsulated flavors	2029:2052	CONCLUSION Wall material formulation affects the retention, morphology and physical properties of the encapsulated flavors, which can be used in food or nutraceutical powder premixes.
31944086	5	80	theme	browning	1000:1007	arg1	extent					973:978	extent	973:978	extent	973:978	Moreover, the morphology and extent of the nonenzymatic browning (NEB) of powder particles were performed using a scanning electron microscope (SEM) and a chroma meter.
31944086	5	80	theme	browning	1000:1007	arg1	morphology					958:967	morphology	958:967	morphology	958:967	Moreover, the morphology and extent of the nonenzymatic browning (NEB) of powder particles were performed using a scanning electron microscope (SEM) and a chroma meter.
31944086	2	81	theme	gum	372:374	arg1	GA					384:385	GA	384:385	GA	384:385	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	2	81	theme	gum	372:374	arg1	arabic					376:381	gum arabic	372:381	gum arabic (GA)	372:386	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	2	81	theme	gum	372:374	arg1	materials					353:361	traditional wall materials	336:361	traditional wall materials	336:361	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	9	82	theme	homogeneous	1683:1693	arg1	capsules					1695:1702	several homogeneous capsules	1675:1702	several homogeneous capsules with a good spherical shape and a smooth surface, according to SEM	1675:1769	The presence of MD as the main encapsulating agent enables the formation of several homogeneous capsules with a good spherical shape and a smooth surface, according to SEM.
31944086	5	83	theme	electron	1067:1074	arg1	SEM					1088:1090	SEM	1088:1090	SEM	1088:1090	Moreover, the morphology and extent of the nonenzymatic browning (NEB) of powder particles were performed using a scanning electron microscope (SEM) and a chroma meter.
31944086	5	83	theme	electron	1067:1074	arg1	microscope					1076:1085	a scanning electron microscope	1056:1085	a scanning electron microscope (SEM)	1056:1091	Moreover, the morphology and extent of the nonenzymatic browning (NEB) of powder particles were performed using a scanning electron microscope (SEM) and a chroma meter.
31944086	7	84	theme	volatile	1412:1419	arg1	compounds					1421:1429	other volatile compounds	1406:1429	other volatile compounds	1406:1429	RESULTS Orange oil shows the maximum retention efficiency (84.5-97.9%), whereas isoamyl acetate is less retained during drying (44.4-72.5%) compared with other volatile compounds.
31944086	9	85	theme	good	1711:1714	arg1	shape					1726:1730	a good spherical shape	1709:1730	a good spherical shape	1709:1730	The presence of MD as the main encapsulating agent enables the formation of several homogeneous capsules with a good spherical shape and a smooth surface, according to SEM.
31944086	3	86	theme	flavor	572:577	arg1	compounds					579:587	flavor compounds	572:587	flavor compounds	572:587	MATERIALS AND METHODS The emulsions of flavor compounds (linalool, citral, orange oil, allyl caproate and isoamyl acetate) were prepared using GA, SC and MD as carriers with different concentrations and then encapsulated in powder form by a spray dryer.
31944086	2	87	theme	wall	471:474	arg1	materials					476:484	different wall materials	461:484	different wall materials	461:484	This study aims to microencapsulate key flavorings by traditional wall materials, namely, gum arabic (GA), maltodextrin (MD) and sodium caseinate (SC) and evaluate the effects of different wall materials on the properties of the flavor microcapsules.
31944086	8	88	theme	highest	1480:1486	arg1	retention					1488:1496	the highest retention	1476:1496	the highest retention for all aroma compounds	1476:1520	Increasing the concentration of GA provides the highest retention for all aroma compounds and improves the viscosity and the emulsion stability of spray-dried powder.
31944086	3	89	theme	allyl	620:624	arg1	MATERIALS					533:541	MATERIALS	533:541	MATERIALS	533:541	MATERIALS AND METHODS The emulsions of flavor compounds (linalool, citral, orange oil, allyl caproate and isoamyl acetate) were prepared using GA, SC and MD as carriers with different concentrations and then encapsulated in powder form by a spray dryer.
31944086	3	89	theme	allyl	620:624	arg1	caproate					626:633	allyl caproate	620:633	allyl caproate	620:633	MATERIALS AND METHODS The emulsions of flavor compounds (linalool, citral, orange oil, allyl caproate and isoamyl acetate) were prepared using GA, SC and MD as carriers with different concentrations and then encapsulated in powder form by a spray dryer.
31944086	4	90	theme	drying	928:933	arg1	process					935:941	the spray drying process	918:941	the spray drying process	918:941	Physical properties, including encapsulation efficiency, viscosity, emulsion stability and moisture, were studied before and after the spray drying process.
31944086	8	91	theme	emulsion	1557:1564	arg1	stability					1566:1574	the emulsion stability	1553:1574	the emulsion stability of spray-dried powder	1553:1596	Increasing the concentration of GA provides the highest retention for all aroma compounds and improves the viscosity and the emulsion stability of spray-dried powder.
32517333	0	0	theme	Modelling	88:96	arg1	Study					98:102	A Molecular Modelling Study	76:102	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.	0:103	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.
32517333	5	1	theme	neoformans	809:818	arg1	D.					836:837	D.	836:837	D.	836:837	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	5	1	theme	neoformans	809:818	arg1	A					830:830	A	830:830	A	830:830	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	5	1	theme	neoformans	809:818	arg1	serotypes					820:828	both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs	801:933	both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs	801:933	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	5	2	theme	molecular	680:688	arg1	simulations					699:709	molecular dynamics simulations	680:709	molecular dynamics simulations	680:709	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	5	3	theme	β	936:936	arg1	DGlcA					938:942	β DGlcA	936:942	β DGlcA	936:942	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	0	4	theme	Molecular	78:86	arg1	Study					98:102	A Molecular Modelling Study	76:102	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.	0:103	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.
32517333	5	5	theme	dynamics	690:697	arg1	simulations					699:709	molecular dynamics simulations	680:709	molecular dynamics simulations	680:709	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	5	6	from	backbone	987:994	arg1	fringes					1016:1022	two hydrophillic fringes	999:1022	two hydrophillic fringes	999:1022	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	6	7	theme	short	1266:1270	arg1	fragments					1276:1284	short GXM fragments	1266:1284	short GXM fragments	1266:1284	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	5	8	theme	simulations	699:709	arg1	array					671:675	an array	668:675	an array of molecular dynamics simulations	668:709	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	6	9	theme	GXM	1272:1274	arg1	fragments					1276:1284	short GXM fragments	1266:1284	short GXM fragments	1266:1284	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	5	10	theme	mannan	980:985	arg1	backbone					987:994	the mannan backbone	976:994	the mannan backbone in two hydrophillic fringes	976:1022	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	6	11	theme	non-protective	1325:1338	arg1	antibodies					1340:1349	non-protective antibodies	1325:1349	non-protective antibodies	1325:1349	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	6	12	theme	clinical	1142:1149	arg1	importance					1168:1177	clinical observations-the importance	1142:1177	clinical observations-the importance of O-acetylation for antibody binding	1142:1215	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	4	13	theme	GXM	630:632	arg1	structure					617:625	the secondary structure	603:625	the secondary structure of GXM	603:632	Although there is evidence for protective antibodies binding conformational epitopes, the secondary structure of GXM remains an unsolved problem.
32517333	6	14	theme	antibody	1200:1207	arg1	binding					1209:1215	antibody binding	1200:1215	antibody binding	1200:1215	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	5	15	dep	D.	836:837	arg1	adds					912:915	adds	912:915	adds structural motifs	912:933	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	5	15	dep	D.	836:837	arg1	alter					870:874	alter	870:874	does not alter the secondary structure	861:898	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	6	16	theme	binding	1230:1236	arg1	self-aggregation					1360:1375	the self-aggregation	1356:1375	the self-aggregation of GXM chains-indicating	1356:1400	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	6	16	theme	binding	1230:1236	arg1	lack					1222:1225	the lack	1218:1225	the lack of binding of protective antibodies to short GXM fragments	1218:1284	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	6	16	theme	binding	1230:1236	arg1	existence					1291:1299	the existence	1287:1299	the existence of epitopes that elicit non-protective antibodies	1287:1349	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	6	16	theme	binding	1230:1236	arg1	rationales					1127:1136	mechanistic rationales	1115:1136	mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding	1115:1215	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	5	17	theme	DXyl	950:953	arg1	chains					960:965	β DXyl side chains	948:965	β DXyl side chains	948:965	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	1	18	theme	immunosuppressed	190:205	arg1	hosts					207:211	immunosuppressed hosts	190:211	immunosuppressed hosts	190:211	The pathogenic encapsulated Cryptococcus neoformans fungus causes serious disease in immunosuppressed hosts.
32517333	5	19	theme	Backbone	839:846	arg1	substitution					848:859	Backbone substitution	839:859	Backbone substitution	839:859	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	6	20	theme	O-acetylation	1182:1194	arg1	importance					1168:1177	clinical observations-the importance	1142:1177	clinical observations-the importance of O-acetylation for antibody binding	1142:1215	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	5	21	theme	hydrophillic	1003:1014	arg1	fringes					1016:1022	two hydrophillic fringes	999:1022	two hydrophillic fringes	999:1022	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	6	22	theme	mechanistic	1115:1125	arg1	rationales					1127:1136	mechanistic rationales	1115:1136	mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding	1115:1215	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	6	23	theme	epitopes	1304:1311	arg1	self-aggregation					1360:1375	the self-aggregation	1356:1375	the self-aggregation of GXM chains-indicating	1356:1400	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	6	23	theme	epitopes	1304:1311	arg1	lack					1222:1225	the lack	1218:1225	the lack of binding of protective antibodies to short GXM fragments	1218:1284	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	6	23	theme	epitopes	1304:1311	arg1	existence					1291:1299	the existence	1287:1299	the existence of epitopes that elicit non-protective antibodies	1287:1349	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	6	23	theme	epitopes	1304:1311	arg1	rationales					1127:1136	mechanistic rationales	1115:1136	mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding	1115:1215	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	6	24	theme	conjugate	1469:1477	arg1	vaccines					1479:1486	conjugate vaccines	1469:1486	conjugate vaccines	1469:1486	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	0	25	theme	Cryptococcus	0:11	arg1	neoformans					13:22	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation	0:73	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.	0:103	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.
32517333	0	25	theme	Cryptococcus	0:11	arg1	Conformation					37:48	Capsular GXM Conformation	24:48	Capsular GXM Conformation	24:48	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.
32517333	0	25	theme	Cryptococcus	0:11	arg1	Presentation					62:73	Epitope Presentation	54:73	Epitope Presentation	54:73	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.
32517333	6	26	theme	molecular	1407:1415	arg1	modelling					1417:1425	molecular modelling	1407:1425	molecular modelling	1407:1425	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	5	27	theme	structural	917:926	arg1	motifs					928:933	structural motifs	917:933	structural motifs	917:933	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	3	28	theme	potential	383:391	arg1	target					401:406	a potential vaccine target	381:406	a potential vaccine target	381:406	While GXM is a potential vaccine target, vaccine development has been confounded by the existence of epitopes that elicit non-protective antibodies.
32517333	3	28	theme	potential	383:391	arg1	GXM					374:376	GXM	374:376	GXM	374:376	While GXM is a potential vaccine target, vaccine development has been confounded by the existence of epitopes that elicit non-protective antibodies.
32517333	3	29	theme	vaccine	393:399	arg1	target					401:406	a potential vaccine target	381:406	a potential vaccine target	381:406	While GXM is a potential vaccine target, vaccine development has been confounded by the existence of epitopes that elicit non-protective antibodies.
32517333	3	29	theme	vaccine	393:399	arg1	GXM					374:376	GXM	374:376	GXM	374:376	While GXM is a potential vaccine target, vaccine development has been confounded by the existence of epitopes that elicit non-protective antibodies.
32517333	4	30	theme	secondary	607:615	arg1	structure					617:625	the secondary structure	603:625	the secondary structure of GXM	603:632	Although there is evidence for protective antibodies binding conformational epitopes, the secondary structure of GXM remains an unsolved problem.
32517333	5	31	dep	serotypes	820:828	arg1	D.					836:837	D.	836:837	D.	836:837	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	5	31	dep	serotypes	820:828	arg1	A					830:830	A	830:830	A	830:830	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	5	31	dep	serotypes	820:828	arg1	serotypes					820:828	both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs	801:933	both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs	801:933	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	5	32	from	inflexible	787:796	arg1	D.					836:837	D.	836:837	D.	836:837	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	5	32	from	inflexible	787:796	arg1	A					830:830	A	830:830	A	830:830	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	5	32	from	inflexible	787:796	arg1	serotypes					820:828	both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs	801:933	both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs	801:933	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	1	33	theme	pathogenic	109:118	arg1	fungus					157:162	The pathogenic encapsulated Cryptococcus neoformans fungus	105:162	The pathogenic encapsulated Cryptococcus neoformans fungus	105:162	The pathogenic encapsulated Cryptococcus neoformans fungus causes serious disease in immunosuppressed hosts.
32517333	5	34	theme	secondary	880:888	arg1	structure					890:898	the secondary structure	876:898	the secondary structure	876:898	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	5	35	theme	β	948:948	arg1	chains					960:965	β DXyl side chains	948:965	β DXyl side chains	948:965	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	6	36	theme	vaccines	1479:1486	arg1	design					1459:1464	the rational design	1446:1464	the rational design of conjugate vaccines	1446:1486	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	6	37	theme	chains-indicating	1384:1400	arg1	self-aggregation					1360:1375	the self-aggregation	1356:1375	the self-aggregation of GXM chains-indicating	1356:1400	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	6	37	theme	chains-indicating	1384:1400	arg1	lack					1222:1225	the lack	1218:1225	the lack of binding of protective antibodies to short GXM fragments	1218:1284	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	6	37	theme	chains-indicating	1384:1400	arg1	existence					1291:1299	the existence	1287:1299	the existence of epitopes that elicit non-protective antibodies	1287:1349	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	6	37	theme	chains-indicating	1384:1400	arg1	rationales					1127:1136	mechanistic rationales	1115:1136	mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding	1115:1215	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	0	38	theme	GXM	33:35	arg1	neoformans					13:22	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation	0:73	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.	0:103	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.
32517333	0	38	theme	GXM	33:35	arg1	Conformation					37:48	Capsular GXM Conformation	24:48	Capsular GXM Conformation	24:48	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.
32517333	3	39	theme	epitopes	469:476	arg1	existence					456:464	the existence	452:464	the existence of epitopes that elicit non-protective antibodies	452:514	While GXM is a potential vaccine target, vaccine development has been confounded by the existence of epitopes that elicit non-protective antibodies.
32517333	3	40	theme	vaccine	409:415	arg1	development					417:427	vaccine development	409:427	vaccine development	409:427	While GXM is a potential vaccine target, vaccine development has been confounded by the existence of epitopes that elicit non-protective antibodies.
32517333	4	41	theme	protective	548:557	arg1	antibodies					559:568	protective antibodies	548:568	protective antibodies binding conformational epitopes	548:600	Although there is evidence for protective antibodies binding conformational epitopes, the secondary structure of GXM remains an unsolved problem.
32517333	6	42	theme	GXM	1380:1382	arg1	chains-indicating					1384:1400	GXM chains-indicating	1380:1400	GXM chains-indicating	1380:1400	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	6	43	theme	rational	1450:1457	arg1	design					1459:1464	the rational design	1446:1464	the rational design of conjugate vaccines	1446:1486	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	5	44	theme	hydrophobic	1064:1074	arg1	ridge					1076:1080	a hydrophobic ridge	1062:1080	a hydrophobic ridge between them	1062:1093	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	1	45	theme	encapsulated	120:131	arg1	neoformans					146:155	encapsulated Cryptococcus neoformans	120:155	The pathogenic encapsulated Cryptococcus neoformans fungus	105:162	The pathogenic encapsulated Cryptococcus neoformans fungus causes serious disease in immunosuppressed hosts.
32517333	0	46	theme	Epitope	54:60	arg1	neoformans					13:22	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation	0:73	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.	0:103	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.
32517333	0	46	theme	Epitope	54:60	arg1	Presentation					62:73	Epitope Presentation	54:73	Epitope Presentation	54:73	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.
32517333	4	47	theme	conformational	578:591	arg1	epitopes					593:600	conformational epitopes	578:600	conformational epitopes	578:600	Although there is evidence for protective antibodies binding conformational epitopes, the secondary structure of GXM remains an unsolved problem.
32517333	2	48	theme	key	229:231	arg1	factor					243:248	a key virulence factor	227:248	a key virulence factor	227:248	The capsule, a key virulence factor, consists primarily of the glucuronoxylomannan polysaccharide (GXM) that varies in composition according to serotype.
32517333	2	48	theme	key	229:231	arg1	capsule					218:224	The capsule	214:224	The capsule	214:224	The capsule, a key virulence factor, consists primarily of the glucuronoxylomannan polysaccharide (GXM) that varies in composition according to serotype.
32517333	2	49	theme	virulence	233:241	arg1	factor					243:248	a key virulence factor	227:248	a key virulence factor	227:248	The capsule, a key virulence factor, consists primarily of the glucuronoxylomannan polysaccharide (GXM) that varies in composition according to serotype.
32517333	2	49	theme	virulence	233:241	arg1	capsule					218:224	The capsule	214:224	The capsule	214:224	The capsule, a key virulence factor, consists primarily of the glucuronoxylomannan polysaccharide (GXM) that varies in composition according to serotype.
32517333	1	50	theme	Cryptococcus	133:144	arg1	neoformans					146:155	encapsulated Cryptococcus neoformans	120:155	The pathogenic encapsulated Cryptococcus neoformans fungus	105:162	The pathogenic encapsulated Cryptococcus neoformans fungus causes serious disease in immunosuppressed hosts.
32517333	6	51	theme	antibodies	1252:1261	arg1	binding					1230:1236	binding	1230:1236	binding of protective antibodies to short GXM fragments	1230:1284	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	5	52	theme	GXM	727:729	arg1	backbone					738:745	the GXM mannan backbone	723:745	the GXM mannan backbone	723:745	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	5	53	from	serotypes	820:828	arg1	inflexible					787:796	inflexible	787:796	inflexible	787:796	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	2	54	theme	glucuronoxylomannan	277:295	arg1	GXM					313:315	GXM	313:315	GXM	313:315	The capsule, a key virulence factor, consists primarily of the glucuronoxylomannan polysaccharide (GXM) that varies in composition according to serotype.
32517333	2	54	theme	glucuronoxylomannan	277:295	arg1	polysaccharide					297:310	the glucuronoxylomannan polysaccharide	273:310	the glucuronoxylomannan polysaccharide (GXM) that varies in composition according to serotype	273:365	The capsule, a key virulence factor, consists primarily of the glucuronoxylomannan polysaccharide (GXM) that varies in composition according to serotype.
32517333	1	55	theme	neoformans	146:155	arg1	fungus					157:162	The pathogenic encapsulated Cryptococcus neoformans fungus	105:162	The pathogenic encapsulated Cryptococcus neoformans fungus	105:162	The pathogenic encapsulated Cryptococcus neoformans fungus causes serious disease in immunosuppressed hosts.
32517333	4	56	theme	unsolved	645:652	arg1	problem					654:660	an unsolved problem	642:660	an unsolved problem	642:660	Although there is evidence for protective antibodies binding conformational epitopes, the secondary structure of GXM remains an unsolved problem.
32517333	5	57	theme	mannan	731:736	arg1	backbone					738:745	the GXM mannan backbone	723:745	the GXM mannan backbone	723:745	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	6	58	theme	protective	1241:1250	arg1	antibodies					1252:1261	protective antibodies	1241:1261	protective antibodies	1241:1261	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	5	59	theme	side	955:958	arg1	chains					960:965	β DXyl side chains	948:965	β DXyl side chains	948:965	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	3	60	theme	non-protective	490:503	arg1	antibodies					505:514	non-protective antibodies	490:514	non-protective antibodies	490:514	While GXM is a potential vaccine target, vaccine development has been confounded by the existence of epitopes that elicit non-protective antibodies.
32517333	6	61	theme	observations-the	1151:1166	arg1	importance					1168:1177	clinical observations-the importance	1142:1177	clinical observations-the importance of O-acetylation for antibody binding	1142:1215	This work provides mechanistic rationales for clinical observations-the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains-indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
32517333	0	62	dep	neoformans	13:22	arg1	neoformans					13:22	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation	0:73	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.	0:103	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.
32517333	0	62	dep	neoformans	13:22	arg1	Study					98:102	A Molecular Modelling Study	76:102	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.	0:103	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.
32517333	0	62	dep	neoformans	13:22	arg1	Conformation					37:48	Capsular GXM Conformation	24:48	Capsular GXM Conformation	24:48	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.
32517333	0	62	dep	neoformans	13:22	arg1	Presentation					62:73	Epitope Presentation	54:73	Epitope Presentation	54:73	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.
32517333	5	63	theme	C.	806:807	arg1	D.					836:837	D.	836:837	D.	836:837	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	5	63	theme	C.	806:807	arg1	A					830:830	A	830:830	A	830:830	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	5	63	theme	C.	806:807	arg1	serotypes					820:828	both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs	801:933	both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs	801:933	Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them.
32517333	0	64	theme	Capsular	24:31	arg1	neoformans					13:22	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation	0:73	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.	0:103	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.
32517333	0	64	theme	Capsular	24:31	arg1	Conformation					37:48	Capsular GXM Conformation	24:48	Capsular GXM Conformation	24:48	Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study.
32517333	1	65	theme	serious	171:177	arg1	disease					179:185	serious disease	171:185	serious disease	171:185	The pathogenic encapsulated Cryptococcus neoformans fungus causes serious disease in immunosuppressed hosts.
35087313	2	0	theme	mannose	342:348	arg1	MR					360:361	MR	360:361	MR	360:361	A paucimannosidic structure, Man3GlcNAc2 (M3), has been reported to enable cellular uptake of glycoproteins through the mannose receptor (MR) in humans, and such uptake has been exploited for the treatment of certain diseases.
35087313	2	0	theme	mannose	342:348	arg1	receptor					350:357	mannose receptor	342:357	the mannose receptor (MR) in humans	338:372	A paucimannosidic structure, Man3GlcNAc2 (M3), has been reported to enable cellular uptake of glycoproteins through the mannose receptor (MR) in humans, and such uptake has been exploited for the treatment of certain diseases.
35087313	2	1	theme	diseases	439:446	arg1	treatment					418:426	the treatment	414:426	the treatment of certain diseases	414:446	A paucimannosidic structure, Man3GlcNAc2 (M3), has been reported to enable cellular uptake of glycoproteins through the mannose receptor (MR) in humans, and such uptake has been exploited for the treatment of certain diseases.
35087313	6	2	theme	human	955:959	arg1	therapy					961:967	human therapy	955:967	human therapy	955:967	Pharmaceutical glycoproteins with these characteristics would be valuable for cellular delivery through the MR, and safe for human therapy.
35087313	2	3	theme	certain	431:437	arg1	diseases					439:446	certain diseases	431:446	certain diseases	431:446	A paucimannosidic structure, Man3GlcNAc2 (M3), has been reported to enable cellular uptake of glycoproteins through the mannose receptor (MR) in humans, and such uptake has been exploited for the treatment of certain diseases.
35087313	5	4	theme	Arabidopsis	664:674	arg1	culture					686:692	Arabidopsis alg3 cell culture	664:692	Arabidopsis alg3 cell culture	664:692	Arabidopsis alg3 cell culture produced glycoproteins with predominantly M3 and GlcNAc-terminal structures, while the amount of plant-specific N-glycans was very low.
35087313	3	5	theme	low	493:495	arg1	level					497:501	a very low level	486:501	a very low level in plants	486:511	However, M3 is generally produced at a very low level in plants.
35087313	5	6	theme	plant-specific	791:804	arg1	N-glycans					806:814	plant-specific N-glycans	791:814	plant-specific N-glycans	791:814	Arabidopsis alg3 cell culture produced glycoproteins with predominantly M3 and GlcNAc-terminal structures, while the amount of plant-specific N-glycans was very low.
35087313	5	7	theme	alg3	676:679	arg1	culture					686:692	Arabidopsis alg3 cell culture	664:692	Arabidopsis alg3 cell culture	664:692	Arabidopsis alg3 cell culture produced glycoproteins with predominantly M3 and GlcNAc-terminal structures, while the amount of plant-specific N-glycans was very low.
35087313	4	8	theme	mutant	585:590	arg1	plant					592:596	an Arabidopsis alg3 mutant plant	565:596	an Arabidopsis alg3 mutant plant lacking asparagine-linked glycosylation 3 (ALG3) enzyme activity	565:661	In this study, a cell culture was established from an Arabidopsis alg3 mutant plant lacking asparagine-linked glycosylation 3 (ALG3) enzyme activity.
35087313	5	9	theme	cell	681:684	arg1	culture					686:692	Arabidopsis alg3 cell culture	664:692	Arabidopsis alg3 cell culture	664:692	Arabidopsis alg3 cell culture produced glycoproteins with predominantly M3 and GlcNAc-terminal structures, while the amount of plant-specific N-glycans was very low.
35087313	1	10	gly	glycoproteins	191:203	arg1	glycoproteins					191:203	pharmaceutical glycoproteins	176:203	pharmaceutical glycoproteins	176:203	N-Glycosylation is essential for protein stability, activity and characteristics, and is often needed to deliver pharmaceutical glycoproteins to target cells.
35087313	5	11	gly	glycoproteins	703:715	arg1	glycoproteins					703:715	glycoproteins	703:715	glycoproteins	703:715	Arabidopsis alg3 cell culture produced glycoproteins with predominantly M3 and GlcNAc-terminal structures, while the amount of plant-specific N-glycans was very low.
35087313	4	12	theme	alg3	580:583	arg1	mutant					585:590	an Arabidopsis alg3 mutant	565:590	an Arabidopsis alg3 mutant plant lacking asparagine-linked glycosylation 3 (ALG3) enzyme activity	565:661	In this study, a cell culture was established from an Arabidopsis alg3 mutant plant lacking asparagine-linked glycosylation 3 (ALG3) enzyme activity.
35087313	6	13	theme	Pharmaceutical	830:843	arg1	glycoproteins					845:857	Pharmaceutical glycoproteins	830:857	Pharmaceutical glycoproteins with these characteristics	830:884	Pharmaceutical glycoproteins with these characteristics would be valuable for cellular delivery through the MR, and safe for human therapy.
35087313	2	14	theme	cellular	297:304	arg1	uptake					306:311	cellular uptake	297:311	cellular uptake of glycoproteins through the mannose receptor (MR) in humans	297:372	A paucimannosidic structure, Man3GlcNAc2 (M3), has been reported to enable cellular uptake of glycoproteins through the mannose receptor (MR) in humans, and such uptake has been exploited for the treatment of certain diseases.
35087313	1	15	theme	pharmaceutical	176:189	arg1	glycoproteins					191:203	pharmaceutical glycoproteins	176:203	pharmaceutical glycoproteins	176:203	N-Glycosylation is essential for protein stability, activity and characteristics, and is often needed to deliver pharmaceutical glycoproteins to target cells.
35087313	0	16	theme	profile	21:27	arg1	Analysis					0:7	Analysis	0:7	Analysis of N-glycan profile of Arabidopsis alg3 cell culture	0:60	Analysis of N-glycan profile of Arabidopsis alg3 cell culture.
35087313	6	17	with	glycoproteins	845:857	arg1	characteristics					870:884	these characteristics	864:884	these characteristics	864:884	Pharmaceutical glycoproteins with these characteristics would be valuable for cellular delivery through the MR, and safe for human therapy.
35087313	2	18	from	receptor	350:357	arg1	humans					367:372	humans	367:372	humans	367:372	A paucimannosidic structure, Man3GlcNAc2 (M3), has been reported to enable cellular uptake of glycoproteins through the mannose receptor (MR) in humans, and such uptake has been exploited for the treatment of certain diseases.
35087313	1	19	theme	protein	96:102	arg1	stability					104:112	protein stability	96:112	protein stability	96:112	N-Glycosylation is essential for protein stability, activity and characteristics, and is often needed to deliver pharmaceutical glycoproteins to target cells.
35087313	0	20	theme	N-glycan	12:19	arg1	profile					21:27	N-glycan profile	12:27	N-glycan profile of Arabidopsis alg3 cell culture	12:60	Analysis of N-glycan profile of Arabidopsis alg3 cell culture.
35087313	4	21	link	asparagine-linked	606:622	arg1	activity					654:661	asparagine-linked glycosylation 3 (ALG3) enzyme activity	606:661	asparagine-linked glycosylation 3 (ALG3) enzyme activity	606:661	In this study, a cell culture was established from an Arabidopsis alg3 mutant plant lacking asparagine-linked glycosylation 3 (ALG3) enzyme activity.
35087313	5	22	theme	GlcNAc-terminal	743:757	arg1	structures					759:768	predominantly M3 and GlcNAc-terminal structures	722:768	predominantly M3 and GlcNAc-terminal structures	722:768	Arabidopsis alg3 cell culture produced glycoproteins with predominantly M3 and GlcNAc-terminal structures, while the amount of plant-specific N-glycans was very low.
35087313	2	23	theme	such	379:382	arg1	uptake					384:389	such uptake	379:389	such uptake	379:389	A paucimannosidic structure, Man3GlcNAc2 (M3), has been reported to enable cellular uptake of glycoproteins through the mannose receptor (MR) in humans, and such uptake has been exploited for the treatment of certain diseases.
35087313	0	24	theme	Arabidopsis	32:42	arg1	culture					54:60	Arabidopsis alg3 cell culture	32:60	Arabidopsis alg3 cell culture	32:60	Analysis of N-glycan profile of Arabidopsis alg3 cell culture.
35087313	4	25	theme	ALG3	641:644	arg1	activity					654:661	asparagine-linked glycosylation 3 (ALG3) enzyme activity	606:661	asparagine-linked glycosylation 3 (ALG3) enzyme activity	606:661	In this study, a cell culture was established from an Arabidopsis alg3 mutant plant lacking asparagine-linked glycosylation 3 (ALG3) enzyme activity.
35087313	4	26	theme	Arabidopsis	568:578	arg1	mutant					585:590	an Arabidopsis alg3 mutant	565:590	an Arabidopsis alg3 mutant plant lacking asparagine-linked glycosylation 3 (ALG3) enzyme activity	565:661	In this study, a cell culture was established from an Arabidopsis alg3 mutant plant lacking asparagine-linked glycosylation 3 (ALG3) enzyme activity.
35087313	1	27	theme	target	208:213	arg1	cells					215:219	target cells	208:219	target cells	208:219	N-Glycosylation is essential for protein stability, activity and characteristics, and is often needed to deliver pharmaceutical glycoproteins to target cells.
35087313	5	28	theme	M3	736:737	arg1	structures					759:768	predominantly M3 and GlcNAc-terminal structures	722:768	predominantly M3 and GlcNAc-terminal structures	722:768	Arabidopsis alg3 cell culture produced glycoproteins with predominantly M3 and GlcNAc-terminal structures, while the amount of plant-specific N-glycans was very low.
35087313	2	29	theme	paucimannosidic	224:238	arg1	Man3GlcNAc2					251:261	Man3GlcNAc2	251:261	Man3GlcNAc2 (M3)	251:266	A paucimannosidic structure, Man3GlcNAc2 (M3), has been reported to enable cellular uptake of glycoproteins through the mannose receptor (MR) in humans, and such uptake has been exploited for the treatment of certain diseases.
35087313	2	29	theme	paucimannosidic	224:238	arg1	structure					240:248	A paucimannosidic structure	222:248	A paucimannosidic structure	222:248	A paucimannosidic structure, Man3GlcNAc2 (M3), has been reported to enable cellular uptake of glycoproteins through the mannose receptor (MR) in humans, and such uptake has been exploited for the treatment of certain diseases.
35087313	5	30	theme	N-glycans	806:814	arg1	amount					781:786	the amount	777:786	the amount of plant-specific N-glycans	777:814	Arabidopsis alg3 cell culture produced glycoproteins with predominantly M3 and GlcNAc-terminal structures, while the amount of plant-specific N-glycans was very low.
35087313	5	30	theme	N-glycans	806:814	arg1	N-glycans					806:814	plant-specific N-glycans	791:814	plant-specific N-glycans	791:814	Arabidopsis alg3 cell culture produced glycoproteins with predominantly M3 and GlcNAc-terminal structures, while the amount of plant-specific N-glycans was very low.
35087313	5	30	theme	N-glycans	806:814	arg1	low					825:827	low	825:827	low	825:827	Arabidopsis alg3 cell culture produced glycoproteins with predominantly M3 and GlcNAc-terminal structures, while the amount of plant-specific N-glycans was very low.
35087313	0	31	theme	cell	49:52	arg1	culture					54:60	Arabidopsis alg3 cell culture	32:60	Arabidopsis alg3 cell culture	32:60	Analysis of N-glycan profile of Arabidopsis alg3 cell culture.
35087313	2	32	gly	glycoproteins	316:328	arg1	glycoproteins					316:328	glycoproteins	316:328	glycoproteins	316:328	A paucimannosidic structure, Man3GlcNAc2 (M3), has been reported to enable cellular uptake of glycoproteins through the mannose receptor (MR) in humans, and such uptake has been exploited for the treatment of certain diseases.
35087313	0	33	theme	alg3	44:47	arg1	culture					54:60	Arabidopsis alg3 cell culture	32:60	Arabidopsis alg3 cell culture	32:60	Analysis of N-glycan profile of Arabidopsis alg3 cell culture.
35087313	6	34	theme	cellular	908:915	arg1	delivery					917:924	cellular delivery	908:924	cellular delivery through the MR	908:939	Pharmaceutical glycoproteins with these characteristics would be valuable for cellular delivery through the MR, and safe for human therapy.
35087313	2	35	theme	glycoproteins	316:328	arg1	uptake					306:311	cellular uptake	297:311	cellular uptake of glycoproteins through the mannose receptor (MR) in humans	297:372	A paucimannosidic structure, Man3GlcNAc2 (M3), has been reported to enable cellular uptake of glycoproteins through the mannose receptor (MR) in humans, and such uptake has been exploited for the treatment of certain diseases.
35087313	3	36	from	level	497:501	arg1	plants					506:511	plants	506:511	plants	506:511	However, M3 is generally produced at a very low level in plants.
35087313	4	37	theme	cell	531:534	arg1	culture					536:542	a cell culture	529:542	a cell culture	529:542	In this study, a cell culture was established from an Arabidopsis alg3 mutant plant lacking asparagine-linked glycosylation 3 (ALG3) enzyme activity.
35087313	4	38	theme	enzyme	647:652	arg1	activity					654:661	asparagine-linked glycosylation 3 (ALG3) enzyme activity	606:661	asparagine-linked glycosylation 3 (ALG3) enzyme activity	606:661	In this study, a cell culture was established from an Arabidopsis alg3 mutant plant lacking asparagine-linked glycosylation 3 (ALG3) enzyme activity.
35087313	4	39	theme	glycosylation	624:636	arg1	activity					654:661	asparagine-linked glycosylation 3 (ALG3) enzyme activity	606:661	asparagine-linked glycosylation 3 (ALG3) enzyme activity	606:661	In this study, a cell culture was established from an Arabidopsis alg3 mutant plant lacking asparagine-linked glycosylation 3 (ALG3) enzyme activity.
35087313	6	40	gly	glycoproteins	845:857	arg1	glycoproteins					845:857	Pharmaceutical glycoproteins	830:857	Pharmaceutical glycoproteins with these characteristics	830:884	Pharmaceutical glycoproteins with these characteristics would be valuable for cellular delivery through the MR, and safe for human therapy.
35087313	0	41	theme	culture	54:60	arg1	profile					21:27	N-glycan profile	12:27	N-glycan profile of Arabidopsis alg3 cell culture	12:60	Analysis of N-glycan profile of Arabidopsis alg3 cell culture.
35087313	4	42	theme	asparagine-linked	606:622	arg1	activity					654:661	asparagine-linked glycosylation 3 (ALG3) enzyme activity	606:661	asparagine-linked glycosylation 3 (ALG3) enzyme activity	606:661	In this study, a cell culture was established from an Arabidopsis alg3 mutant plant lacking asparagine-linked glycosylation 3 (ALG3) enzyme activity.
33737176	0	0	theme	thermo-mechanical	77:93	arg1	properties					95:104	thermo-mechanical properties	77:104	thermo-mechanical properties	77:104	Effect of silane modified microcrystalline cellulose on the curing kinetics, thermo-mechanical properties and thermal degradation of benzoxazine resin.
33737176	4	1	theme	curing	982:987	arg1	mechanism					989:997	an autocatalytic curing mechanism	965:997	an autocatalytic curing mechanism	965:997	The kinetic data revealed a decrease in the average values of activation energy and the pre-exponential factor, particularly for composite supplemented with 10% MCC Si, whereas all samples disclosed a tendency of an autocatalytic curing mechanism.
33737176	4	2	dep	revealed	769:776	arg1	whereas					921:927	whereas	921:927	whereas	921:927	The kinetic data revealed a decrease in the average values of activation energy and the pre-exponential factor, particularly for composite supplemented with 10% MCC Si, whereas all samples disclosed a tendency of an autocatalytic curing mechanism.
33737176	5	3	theme	mechanical	1038:1047	arg1	properties					1049:1058	dynamic mechanical properties	1030:1058	dynamic mechanical properties	1030:1058	Furthermore, the study of the dynamic mechanical properties and degradation features of the cured specimens, respectively, indicated a superior stiffness attributable to the good interaction between BA-a and MCC Si, and enhanced thermal stability for the composites compared to pristine resin.
33737176	5	4	dep	properties	1049:1058	arg1	the					1026:1028	the	1026:1028	the	1026:1028	Furthermore, the study of the dynamic mechanical properties and degradation features of the cured specimens, respectively, indicated a superior stiffness attributable to the good interaction between BA-a and MCC Si, and enhanced thermal stability for the composites compared to pristine resin.
33737176	2	5	theme	morphological	450:462	arg1	characterizations					482:498	The structural, morphological and crystallinity characterizations	434:498	The structural, morphological and crystallinity characterizations of the modified MCC	434:518	The structural, morphological and crystallinity characterizations of the modified MCC were initially performed to scrutinize the changes and confirm the modification.
33737176	1	6	used	used	315:318	arg2	agent					337:341	a reinforcing agent	323:341	a reinforcing agent of benzoxazine resin	323:362	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	1	6	used	used	315:318	arg2	cellulose					254:262	microcrystalline cellulose	237:262	microcrystalline cellulose modified through silane coupling agent (MCC Si)	237:310	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	4	7	from	decrease	780:787	arg1	values					804:809	the average values	792:809	the average values of activation energy	792:830	The kinetic data revealed a decrease in the average values of activation energy and the pre-exponential factor, particularly for composite supplemented with 10% MCC Si, whereas all samples disclosed a tendency of an autocatalytic curing mechanism.
33737176	1	8	theme	silane	281:286	arg1	Si					308:309	MCC Si	304:309	MCC Si	304:309	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	1	8	theme	silane	281:286	arg1	agent					297:301	silane coupling agent	281:301	silane coupling agent (MCC Si)	281:310	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	2	9	theme	MCC	516:518	arg1	characterizations					482:498	The structural, morphological and crystallinity characterizations	434:498	The structural, morphological and crystallinity characterizations of the modified MCC	434:518	The structural, morphological and crystallinity characterizations of the modified MCC were initially performed to scrutinize the changes and confirm the modification.
33737176	1	10	theme	benzoxazine	346:356	arg1	resin					358:362	benzoxazine resin	346:362	benzoxazine resin	346:362	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	1	11	theme	coupling	288:295	arg1	Si					308:309	MCC Si	304:309	MCC Si	304:309	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	1	11	theme	coupling	288:295	arg1	agent					297:301	silane coupling agent	281:301	silane coupling agent (MCC Si)	281:310	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	2	12	theme	modified	507:514	arg1	MCC					516:518	the modified MCC	503:518	the modified MCC	503:518	The structural, morphological and crystallinity characterizations of the modified MCC were initially performed to scrutinize the changes and confirm the modification.
33737176	1	13	theme	resin	358:362	arg1	cellulose					254:262	microcrystalline cellulose	237:262	microcrystalline cellulose modified through silane coupling agent (MCC Si)	237:310	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	1	13	theme	resin	358:362	arg1	agent					337:341	a reinforcing agent	323:341	a reinforcing agent of benzoxazine resin	323:362	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	4	14	theme	mechanism	989:997	arg1	tendency					953:960	a tendency	951:960	a tendency of an autocatalytic curing mechanism	951:997	The kinetic data revealed a decrease in the average values of activation energy and the pre-exponential factor, particularly for composite supplemented with 10% MCC Si, whereas all samples disclosed a tendency of an autocatalytic curing mechanism.
33737176	3	15	theme	isoconversional	727:741	arg1	methods					743:749	isoconversional methods	727:749	isoconversional methods	727:749	Then, an investigation on the crosslinking process of the prepared composites was held through curing kinetic study employing isoconversional methods.
33737176	4	16	theme	pre-exponential	840:854	arg1	factor					856:861	the pre-exponential factor	836:861	the pre-exponential factor	836:861	The kinetic data revealed a decrease in the average values of activation energy and the pre-exponential factor, particularly for composite supplemented with 10% MCC Si, whereas all samples disclosed a tendency of an autocatalytic curing mechanism.
33737176	1	17	theme	%	431:431	arg1	loadings					403:410	different loadings	393:410	different loadings of 5, 10, 15, 20 wt%	393:431	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	0	18	theme	thermal	110:116	arg1	degradation					118:128	thermal degradation	110:128	thermal degradation	110:128	Effect of silane modified microcrystalline cellulose on the curing kinetics, thermo-mechanical properties and thermal degradation of benzoxazine resin.
33737176	3	19	theme	kinetic	703:709	arg1	study					711:715	kinetic study	703:715	kinetic study employing isoconversional methods	703:749	Then, an investigation on the crosslinking process of the prepared composites was held through curing kinetic study employing isoconversional methods.
33737176	5	20	theme	cured	1092:1096	arg1	specimens					1098:1106	the cured specimens	1088:1106	the cured specimens	1088:1106	Furthermore, the study of the dynamic mechanical properties and degradation features of the cured specimens, respectively, indicated a superior stiffness attributable to the good interaction between BA-a and MCC Si, and enhanced thermal stability for the composites compared to pristine resin.
33737176	4	21	from	factor	856:861	arg1	values					804:809	the average values	792:809	the average values of activation energy	792:830	The kinetic data revealed a decrease in the average values of activation energy and the pre-exponential factor, particularly for composite supplemented with 10% MCC Si, whereas all samples disclosed a tendency of an autocatalytic curing mechanism.
33737176	1	22	theme	sustainable	179:189	arg1	materials					226:234	sustainable, eco-friendly and high performance materials	179:234	sustainable, eco-friendly and high performance materials	179:234	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	2	23	theme	structural	438:447	arg1	characterizations					482:498	The structural, morphological and crystallinity characterizations	434:498	The structural, morphological and crystallinity characterizations of the modified MCC	434:518	The structural, morphological and crystallinity characterizations of the modified MCC were initially performed to scrutinize the changes and confirm the modification.
33737176	4	24	theme	autocatalytic	968:980	arg1	mechanism					989:997	an autocatalytic curing mechanism	965:997	an autocatalytic curing mechanism	965:997	The kinetic data revealed a decrease in the average values of activation energy and the pre-exponential factor, particularly for composite supplemented with 10% MCC Si, whereas all samples disclosed a tendency of an autocatalytic curing mechanism.
33737176	3	25	from	investigation	610:622	arg1	process					644:650	the crosslinking process	627:650	the crosslinking process of the prepared composites	627:677	Then, an investigation on the crosslinking process of the prepared composites was held through curing kinetic study employing isoconversional methods.
33737176	5	26	theme	good	1174:1177	arg1	interaction					1179:1189	the good interaction	1170:1189	the good interaction between BA-a and MCC Si	1170:1213	Furthermore, the study of the dynamic mechanical properties and degradation features of the cured specimens, respectively, indicated a superior stiffness attributable to the good interaction between BA-a and MCC Si, and enhanced thermal stability for the composites compared to pristine resin.
33737176	5	27	theme	dynamic	1030:1036	arg1	properties					1049:1058	dynamic mechanical properties	1030:1058	dynamic mechanical properties	1030:1058	Furthermore, the study of the dynamic mechanical properties and degradation features of the cured specimens, respectively, indicated a superior stiffness attributable to the good interaction between BA-a and MCC Si, and enhanced thermal stability for the composites compared to pristine resin.
33737176	1	28	theme	eco-friendly	192:203	arg1	materials					226:234	sustainable, eco-friendly and high performance materials	179:234	sustainable, eco-friendly and high performance materials	179:234	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	0	29	theme	modified	17:24	arg1	cellulose					43:51	silane modified microcrystalline cellulose	10:51	silane modified microcrystalline cellulose	10:51	Effect of silane modified microcrystalline cellulose on the curing kinetics, thermo-mechanical properties and thermal degradation of benzoxazine resin.
33737176	1	30	theme	MCC	304:306	arg1	Si					308:309	MCC Si	304:309	MCC Si	304:309	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	1	30	theme	MCC	304:306	arg1	agent					297:301	silane coupling agent	281:301	silane coupling agent (MCC Si)	281:310	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	0	31	theme	benzoxazine	133:143	arg1	resin					145:149	benzoxazine resin	133:149	benzoxazine resin	133:149	Effect of silane modified microcrystalline cellulose on the curing kinetics, thermo-mechanical properties and thermal degradation of benzoxazine resin.
33737176	4	32	theme	kinetic	756:762	arg1	data					764:767	The kinetic data	752:767	The kinetic data	752:767	The kinetic data revealed a decrease in the average values of activation energy and the pre-exponential factor, particularly for composite supplemented with 10% MCC Si, whereas all samples disclosed a tendency of an autocatalytic curing mechanism.
33737176	0	33	theme	silane	10:15	arg1	cellulose					43:51	silane modified microcrystalline cellulose	10:51	silane modified microcrystalline cellulose	10:51	Effect of silane modified microcrystalline cellulose on the curing kinetics, thermo-mechanical properties and thermal degradation of benzoxazine resin.
33737176	3	34	theme	crosslinking	631:642	arg1	process					644:650	the crosslinking process	627:650	the crosslinking process of the prepared composites	627:677	Then, an investigation on the crosslinking process of the prepared composites was held through curing kinetic study employing isoconversional methods.
33737176	1	35	theme	high	209:212	arg1	performance					214:224	high performance	209:224	high performance	209:224	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	0	36	theme	cellulose	43:51	arg1	Effect					0:5	Effect	0:5	Effect of silane modified microcrystalline cellulose on the curing kinetics, thermo-mechanical properties and thermal degradation of benzoxazine resin.	0:150	Effect of silane modified microcrystalline cellulose on the curing kinetics, thermo-mechanical properties and thermal degradation of benzoxazine resin.
33737176	1	37	theme	different	393:401	arg1	loadings					403:410	different loadings	393:410	different loadings of 5, 10, 15, 20 wt%	393:431	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	4	38	theme	activation	814:823	arg1	energy					825:830	activation energy	814:830	activation energy	814:830	The kinetic data revealed a decrease in the average values of activation energy and the pre-exponential factor, particularly for composite supplemented with 10% MCC Si, whereas all samples disclosed a tendency of an autocatalytic curing mechanism.
33737176	1	39	theme	performance	214:224	arg1	materials					226:234	sustainable, eco-friendly and high performance materials	179:234	sustainable, eco-friendly and high performance materials	179:234	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	0	40	theme	microcrystalline	26:41	arg1	cellulose					43:51	silane modified microcrystalline cellulose	10:51	silane modified microcrystalline cellulose	10:51	Effect of silane modified microcrystalline cellulose on the curing kinetics, thermo-mechanical properties and thermal degradation of benzoxazine resin.
33737176	0	41	theme	resin	145:149	arg1	kinetics					67:74	the curing kinetics	56:74	the curing kinetics	56:74	Effect of silane modified microcrystalline cellulose on the curing kinetics, thermo-mechanical properties and thermal degradation of benzoxazine resin.
33737176	0	41	theme	resin	145:149	arg1	properties					95:104	thermo-mechanical properties	77:104	thermo-mechanical properties	77:104	Effect of silane modified microcrystalline cellulose on the curing kinetics, thermo-mechanical properties and thermal degradation of benzoxazine resin.
33737176	0	41	theme	resin	145:149	arg1	degradation					118:128	thermal degradation	110:128	thermal degradation	110:128	Effect of silane modified microcrystalline cellulose on the curing kinetics, thermo-mechanical properties and thermal degradation of benzoxazine resin.
33737176	5	42	theme	degradation	1064:1074	arg1	features					1076:1083	degradation features	1064:1083	degradation features	1064:1083	Furthermore, the study of the dynamic mechanical properties and degradation features of the cured specimens, respectively, indicated a superior stiffness attributable to the good interaction between BA-a and MCC Si, and enhanced thermal stability for the composites compared to pristine resin.
33737176	5	43	theme	attributable	1154:1165	arg1	stiffness					1144:1152	a superior stiffness	1133:1152	a superior stiffness attributable to the good interaction between BA-a and MCC Si	1133:1213	Furthermore, the study of the dynamic mechanical properties and degradation features of the cured specimens, respectively, indicated a superior stiffness attributable to the good interaction between BA-a and MCC Si, and enhanced thermal stability for the composites compared to pristine resin.
33737176	4	44	theme	10	909:910	arg1	%					911:911	%	911:911	%	911:911	The kinetic data revealed a decrease in the average values of activation energy and the pre-exponential factor, particularly for composite supplemented with 10% MCC Si, whereas all samples disclosed a tendency of an autocatalytic curing mechanism.
33737176	2	45	theme	crystallinity	468:480	arg1	characterizations					482:498	The structural, morphological and crystallinity characterizations	434:498	The structural, morphological and crystallinity characterizations of the modified MCC	434:518	The structural, morphological and crystallinity characterizations of the modified MCC were initially performed to scrutinize the changes and confirm the modification.
33737176	0	46	from	Effect	0:5	arg1	kinetics					67:74	the curing kinetics	56:74	the curing kinetics	56:74	Effect of silane modified microcrystalline cellulose on the curing kinetics, thermo-mechanical properties and thermal degradation of benzoxazine resin.
33737176	0	46	from	Effect	0:5	arg1	properties					95:104	thermo-mechanical properties	77:104	thermo-mechanical properties	77:104	Effect of silane modified microcrystalline cellulose on the curing kinetics, thermo-mechanical properties and thermal degradation of benzoxazine resin.
33737176	0	46	from	Effect	0:5	arg1	degradation					118:128	thermal degradation	110:128	thermal degradation	110:128	Effect of silane modified microcrystalline cellulose on the curing kinetics, thermo-mechanical properties and thermal degradation of benzoxazine resin.
33737176	3	47	theme	prepared	659:666	arg1	composites					668:677	the prepared composites	655:677	the prepared composites	655:677	Then, an investigation on the crosslinking process of the prepared composites was held through curing kinetic study employing isoconversional methods.
33737176	4	48	theme	energy	825:830	arg1	values					804:809	the average values	792:809	the average values of activation energy	792:830	The kinetic data revealed a decrease in the average values of activation energy and the pre-exponential factor, particularly for composite supplemented with 10% MCC Si, whereas all samples disclosed a tendency of an autocatalytic curing mechanism.
33737176	4	49	theme	MCC	913:915	arg1	Si					917:918	10% MCC Si	909:918	10% MCC Si	909:918	The kinetic data revealed a decrease in the average values of activation energy and the pre-exponential factor, particularly for composite supplemented with 10% MCC Si, whereas all samples disclosed a tendency of an autocatalytic curing mechanism.
33737176	3	50	theme	composites	668:677	arg1	process					644:650	the crosslinking process	627:650	the crosslinking process of the prepared composites	627:677	Then, an investigation on the crosslinking process of the prepared composites was held through curing kinetic study employing isoconversional methods.
33737176	5	51	theme	specimens	1098:1106	arg1	properties					1049:1058	dynamic mechanical properties	1030:1058	dynamic mechanical properties	1030:1058	Furthermore, the study of the dynamic mechanical properties and degradation features of the cured specimens, respectively, indicated a superior stiffness attributable to the good interaction between BA-a and MCC Si, and enhanced thermal stability for the composites compared to pristine resin.
33737176	5	51	theme	specimens	1098:1106	arg1	features					1076:1083	degradation features	1064:1083	degradation features	1064:1083	Furthermore, the study of the dynamic mechanical properties and degradation features of the cured specimens, respectively, indicated a superior stiffness attributable to the good interaction between BA-a and MCC Si, and enhanced thermal stability for the composites compared to pristine resin.
33737176	5	52	theme	pristine	1278:1285	arg1	resin					1287:1291	pristine resin	1278:1291	pristine resin	1278:1291	Furthermore, the study of the dynamic mechanical properties and degradation features of the cured specimens, respectively, indicated a superior stiffness attributable to the good interaction between BA-a and MCC Si, and enhanced thermal stability for the composites compared to pristine resin.
33737176	4	53	theme	%	911:911	arg1	Si					917:918	10% MCC Si	909:918	10% MCC Si	909:918	The kinetic data revealed a decrease in the average values of activation energy and the pre-exponential factor, particularly for composite supplemented with 10% MCC Si, whereas all samples disclosed a tendency of an autocatalytic curing mechanism.
33737176	5	54	theme	properties	1049:1058	arg1	study					1017:1021	the study	1013:1021	the study of the dynamic mechanical properties and degradation features of the cured specimens, respectively,	1013:1121	Furthermore, the study of the dynamic mechanical properties and degradation features of the cured specimens, respectively, indicated a superior stiffness attributable to the good interaction between BA-a and MCC Si, and enhanced thermal stability for the composites compared to pristine resin.
33737176	5	55	theme	features	1076:1083	arg1	study					1017:1021	the study	1013:1021	the study of the dynamic mechanical properties and degradation features of the cured specimens, respectively,	1013:1121	Furthermore, the study of the dynamic mechanical properties and degradation features of the cured specimens, respectively, indicated a superior stiffness attributable to the good interaction between BA-a and MCC Si, and enhanced thermal stability for the composites compared to pristine resin.
33737176	1	56	theme	microcrystalline	237:252	arg1	cellulose					254:262	microcrystalline cellulose	237:262	microcrystalline cellulose modified through silane coupling agent (MCC Si)	237:310	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	1	56	theme	microcrystalline	237:252	arg1	agent					337:341	a reinforcing agent	323:341	a reinforcing agent of benzoxazine resin	323:362	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	5	57	theme	superior	1135:1142	arg1	stiffness					1144:1152	a superior stiffness	1133:1152	a superior stiffness attributable to the good interaction between BA-a and MCC Si	1133:1213	Furthermore, the study of the dynamic mechanical properties and degradation features of the cured specimens, respectively, indicated a superior stiffness attributable to the good interaction between BA-a and MCC Si, and enhanced thermal stability for the composites compared to pristine resin.
33737176	5	58	theme	thermal	1229:1235	arg1	stability					1237:1245	thermal stability	1229:1245	thermal stability for the composites compared to pristine resin	1229:1291	Furthermore, the study of the dynamic mechanical properties and degradation features of the cured specimens, respectively, indicated a superior stiffness attributable to the good interaction between BA-a and MCC Si, and enhanced thermal stability for the composites compared to pristine resin.
33737176	0	59	theme	curing	60:65	arg1	kinetics					67:74	the curing kinetics	56:74	the curing kinetics	56:74	Effect of silane modified microcrystalline cellulose on the curing kinetics, thermo-mechanical properties and thermal degradation of benzoxazine resin.
33737176	1	60	theme	reinforcing	325:335	arg1	cellulose					254:262	microcrystalline cellulose	237:262	microcrystalline cellulose modified through silane coupling agent (MCC Si)	237:310	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	1	60	theme	reinforcing	325:335	arg1	agent					337:341	a reinforcing agent	323:341	a reinforcing agent of benzoxazine resin	323:362	In the frame of developing sustainable, eco-friendly and high performance materials, microcrystalline cellulose modified through silane coupling agent (MCC Si) is used as a reinforcing agent of benzoxazine resin to manufacture composites at different loadings of 5, 10, 15, 20 wt%.
33737176	4	61	theme	average	796:802	arg1	values					804:809	the average values	792:809	the average values of activation energy	792:830	The kinetic data revealed a decrease in the average values of activation energy and the pre-exponential factor, particularly for composite supplemented with 10% MCC Si, whereas all samples disclosed a tendency of an autocatalytic curing mechanism.
33737176	5	62	theme	MCC	1208:1210	arg1	Si					1212:1213	MCC Si	1208:1213	MCC Si	1208:1213	Furthermore, the study of the dynamic mechanical properties and degradation features of the cured specimens, respectively, indicated a superior stiffness attributable to the good interaction between BA-a and MCC Si, and enhanced thermal stability for the composites compared to pristine resin.
33445012	4	0	dep	sources	818:824	arg1	galactose					836:844	galactose	836:844	galactose	836:844	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	0	dep	sources	818:824	arg1	glycerol					847:854	glycerol	847:854	glycerol	847:854	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	0	dep	sources	818:824	arg1	xylose					879:884	xylose	879:884	xylose	879:884	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	0	dep	sources	818:824	arg1	sources					818:824	different carbon sources	801:824	different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose)	801:885	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	0	dep	sources	818:824	arg1	glucose					827:833	glucose	827:833	glucose	827:833	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	0	dep	sources	818:824	arg1	lactose					857:863	lactose	857:863	lactose	857:863	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	0	dep	sources	818:824	arg1	sucrose					866:872	sucrose	866:872	sucrose	866:872	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	3	1	theme	main	482:485	arg1	source					494:499	the main carbon source	478:499	the main carbon source	478:499	Purified EPS produced using lactose as the main carbon source was analyzed by GC-MS and found to be composed of α-D-xylopyranose (28.6 ± 2.0%), β-D-glucopyranose (21.0 ± 1.6%), α-D-mannopyranose (18.5 ± 1.2%), β-d-mannopyranose (13.1 ± 1.4%), β-D-xylopyranose (10.2 ± 2.1%), α-d-talopyranose (5.9 ± 1.1%), and β-d-galacturonic acid (2.43 ± 0.8%).
33445012	3	1	theme	main	482:485	arg1	lactose					467:473	lactose	467:473	lactose	467:473	Purified EPS produced using lactose as the main carbon source was analyzed by GC-MS and found to be composed of α-D-xylopyranose (28.6 ± 2.0%), β-D-glucopyranose (21.0 ± 1.6%), α-D-mannopyranose (18.5 ± 1.2%), β-d-mannopyranose (13.1 ± 1.4%), β-D-xylopyranose (10.2 ± 2.1%), α-d-talopyranose (5.9 ± 1.1%), and β-d-galacturonic acid (2.43 ± 0.8%).
33445012	0	2	theme	exopolysaccharide	18:34	arg1	Bioprospecting					0:13	Bioprospecting	0:13	Bioprospecting of exopolysaccharide from marine Sphingobium yanoikuyae BBL01: Production, characterization, and metal chelation activity.	0:136	Bioprospecting of exopolysaccharide from marine Sphingobium yanoikuyae BBL01: Production, characterization, and metal chelation activity.
33445012	3	3	theme	β-d-galacturonic	749:764	arg1	%					782:782	2.43 ± 0.8%	772:782	2.43 ± 0.8%	772:782	Purified EPS produced using lactose as the main carbon source was analyzed by GC-MS and found to be composed of α-D-xylopyranose (28.6 ± 2.0%), β-D-glucopyranose (21.0 ± 1.6%), α-D-mannopyranose (18.5 ± 1.2%), β-d-mannopyranose (13.1 ± 1.4%), β-D-xylopyranose (10.2 ± 2.1%), α-d-talopyranose (5.9 ± 1.1%), and β-d-galacturonic acid (2.43 ± 0.8%).
33445012	3	3	theme	β-d-galacturonic	749:764	arg1	acid					766:769	β-d-galacturonic acid	749:769	β-d-galacturonic acid (2.43 ± 0.8%)	749:783	Purified EPS produced using lactose as the main carbon source was analyzed by GC-MS and found to be composed of α-D-xylopyranose (28.6 ± 2.0%), β-D-glucopyranose (21.0 ± 1.6%), α-D-mannopyranose (18.5 ± 1.2%), β-d-mannopyranose (13.1 ± 1.4%), β-D-xylopyranose (10.2 ± 2.1%), α-d-talopyranose (5.9 ± 1.1%), and β-d-galacturonic acid (2.43 ± 0.8%).
33445012	6	4	theme	emulsifying	1172:1182	arg1	scavenging					1212:1221	considerable emulsifying, flocculating, free-radical scavenging, and metal-complexion activity	1159:1252	scavenging	1212:1221	EPS also showed considerable emulsifying, flocculating, free-radical scavenging, and metal-complexion activity, suggesting various biotechnological applications.
33445012	3	5	theme	carbon	487:492	arg1	source					494:499	the main carbon source	478:499	the main carbon source	478:499	Purified EPS produced using lactose as the main carbon source was analyzed by GC-MS and found to be composed of α-D-xylopyranose (28.6 ± 2.0%), β-D-glucopyranose (21.0 ± 1.6%), α-D-mannopyranose (18.5 ± 1.2%), β-d-mannopyranose (13.1 ± 1.4%), β-D-xylopyranose (10.2 ± 2.1%), α-d-talopyranose (5.9 ± 1.1%), and β-d-galacturonic acid (2.43 ± 0.8%).
33445012	3	5	theme	carbon	487:492	arg1	lactose					467:473	lactose	467:473	lactose	467:473	Purified EPS produced using lactose as the main carbon source was analyzed by GC-MS and found to be composed of α-D-xylopyranose (28.6 ± 2.0%), β-D-glucopyranose (21.0 ± 1.6%), α-D-mannopyranose (18.5 ± 1.2%), β-d-mannopyranose (13.1 ± 1.4%), β-D-xylopyranose (10.2 ± 2.1%), α-d-talopyranose (5.9 ± 1.1%), and β-d-galacturonic acid (2.43 ± 0.8%).
33445012	6	6	theme	biotechnological	1274:1289	arg1	applications					1291:1302	various biotechnological applications	1266:1302	various biotechnological applications	1266:1302	EPS also showed considerable emulsifying, flocculating, free-radical scavenging, and metal-complexion activity, suggesting various biotechnological applications.
33445012	4	7	theme	different	801:809	arg1	galactose					836:844	galactose	836:844	galactose	836:844	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	7	theme	different	801:809	arg1	glycerol					847:854	glycerol	847:854	glycerol	847:854	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	7	theme	different	801:809	arg1	xylose					879:884	xylose	879:884	xylose	879:884	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	7	theme	different	801:809	arg1	sources					818:824	different carbon sources	801:824	different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose)	801:885	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	7	theme	different	801:809	arg1	glucose					827:833	glucose	827:833	glucose	827:833	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	7	theme	different	801:809	arg1	lactose					857:863	lactose	857:863	lactose	857:863	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	7	theme	different	801:809	arg1	sucrose					866:872	sucrose	866:872	sucrose	866:872	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	8	theme	slight	939:944	arg1	change					946:951	a slight change	937:951	a slight change in the mass percentage of various monosaccharides	937:1001	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	5	9	theme	thickening	1083:1092	arg1	agent					1106:1110	a thickening and gelling agent	1081:1110	a thickening and gelling agent for food-related applications	1081:1140	Purified EPS was stable up to 233 °C, indicating its possible suitability as a thickening and gelling agent for food-related applications.
33445012	5	9	theme	thickening	1083:1092	arg1	suitability					1066:1076	its possible suitability	1053:1076	its possible suitability	1053:1076	Purified EPS was stable up to 233 °C, indicating its possible suitability as a thickening and gelling agent for food-related applications.
33445012	6	10	theme	various	1266:1272	arg1	applications					1291:1302	various biotechnological applications	1266:1302	various biotechnological applications	1266:1302	EPS also showed considerable emulsifying, flocculating, free-radical scavenging, and metal-complexion activity, suggesting various biotechnological applications.
33445012	0	11	from	BBL01	71:75	arg1	Bioprospecting					0:13	Bioprospecting	0:13	Bioprospecting of exopolysaccharide from marine Sphingobium yanoikuyae BBL01: Production, characterization, and metal chelation activity.	0:136	Bioprospecting of exopolysaccharide from marine Sphingobium yanoikuyae BBL01: Production, characterization, and metal chelation activity.
33445012	5	12	theme	food-related	1116:1127	arg1	applications					1129:1140	food-related applications	1116:1140	food-related applications	1116:1140	Purified EPS was stable up to 233 °C, indicating its possible suitability as a thickening and gelling agent for food-related applications.
33445012	0	13	theme	chelation	118:126	arg1	activity					128:135	metal chelation activity	112:135	metal chelation activity	112:135	Bioprospecting of exopolysaccharide from marine Sphingobium yanoikuyae BBL01: Production, characterization, and metal chelation activity.
33445012	1	14	dep	Sphingobium	296:306	arg1	yanoikuyae					308:317	yanoikuyae	308:317	yanoikuyae	308:317	In the present study, an exopolysaccharide (EPS)-producing bacterial strain was isolated from the Eastern Sea (Sokcho Beach) of South Korea and identified as Sphingobium yanoikuyae BBL01.
33445012	0	15	theme	metal	112:116	arg1	chelation					118:126	metal chelation	112:126	metal chelation activity	112:135	Bioprospecting of exopolysaccharide from marine Sphingobium yanoikuyae BBL01: Production, characterization, and metal chelation activity.
33445012	5	16	theme	Purified	1004:1011	arg1	EPS					1013:1015	Purified EPS	1004:1015	Purified EPS	1004:1015	Purified EPS was stable up to 233 °C, indicating its possible suitability as a thickening and gelling agent for food-related applications.
33445012	5	17	dep	233 °C	1034:1039	arg1	up					1028:1029	up	1028:1029	up	1028:1029	Purified EPS was stable up to 233 °C, indicating its possible suitability as a thickening and gelling agent for food-related applications.
33445012	4	18	theme	monomer	911:917	arg1	composition					919:929	EPS monomer composition	907:929	EPS monomer composition	907:929	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	2	19	theme	surface	374:380	arg1	design					382:387	response surface design	365:387	response surface design	365:387	Media optimization was performed using response surface design, and a yield of 2.63 ± 0.02 g/L EPS was achieved.
33445012	4	20	theme	EPS	907:909	arg1	composition					919:929	EPS monomer composition	907:929	EPS monomer composition	907:929	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	21	theme	various	979:985	arg1	monosaccharides					987:1001	various monosaccharides	979:1001	various monosaccharides	979:1001	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	2	22	theme	response	365:372	arg1	design					382:387	response surface design	365:387	response surface design	365:387	Media optimization was performed using response surface design, and a yield of 2.63 ± 0.02 g/L EPS was achieved.
33445012	2	23	theme	EPS	421:423	arg1	yield					396:400	a yield	394:400	a yield of 2.63 ± 0.02 g/L EPS	394:423	Media optimization was performed using response surface design, and a yield of 2.63 ± 0.02 g/L EPS was achieved.
33445012	2	24	theme	2.63 ± 0.02 g/L	405:419	arg1	EPS					421:423	2.63 ± 0.02 g/L EPS	405:423	2.63 ± 0.02 g/L EPS	405:423	Media optimization was performed using response surface design, and a yield of 2.63 ± 0.02 g/L EPS was achieved.
33445012	5	25	theme	possible	1057:1064	arg1	agent					1106:1110	a thickening and gelling agent	1081:1110	a thickening and gelling agent for food-related applications	1081:1140	Purified EPS was stable up to 233 °C, indicating its possible suitability as a thickening and gelling agent for food-related applications.
33445012	5	25	theme	possible	1057:1064	arg1	suitability					1066:1076	its possible suitability	1053:1076	its possible suitability	1053:1076	Purified EPS was stable up to 233 °C, indicating its possible suitability as a thickening and gelling agent for food-related applications.
33445012	6	26	theme	metal-complexion	1228:1243	arg1	activity					1245:1252	considerable emulsifying, flocculating, free-radical scavenging, and metal-complexion activity	1159:1252	activity	1245:1252	EPS also showed considerable emulsifying, flocculating, free-radical scavenging, and metal-complexion activity, suggesting various biotechnological applications.
33445012	1	27	theme	bacterial	197:205	arg1	strain					207:212	bacterial strain	197:212	bacterial strain	197:212	In the present study, an exopolysaccharide (EPS)-producing bacterial strain was isolated from the Eastern Sea (Sokcho Beach) of South Korea and identified as Sphingobium yanoikuyae BBL01.
33445012	0	28	theme	Sphingobium	48:58	arg1	BBL01					71:75	marine Sphingobium yanoikuyae BBL01	41:75	marine Sphingobium yanoikuyae BBL01	41:75	Bioprospecting of exopolysaccharide from marine Sphingobium yanoikuyae BBL01: Production, characterization, and metal chelation activity.
33445012	1	29	theme	Korea	272:276	arg1	Sea					244:246	the Eastern Sea	232:246	the Eastern Sea (Sokcho Beach) of South Korea	232:276	In the present study, an exopolysaccharide (EPS)-producing bacterial strain was isolated from the Eastern Sea (Sokcho Beach) of South Korea and identified as Sphingobium yanoikuyae BBL01.
33445012	1	29	theme	Korea	272:276	arg1	Beach					256:260	Beach	256:260	Beach	256:260	In the present study, an exopolysaccharide (EPS)-producing bacterial strain was isolated from the Eastern Sea (Sokcho Beach) of South Korea and identified as Sphingobium yanoikuyae BBL01.
33445012	4	30	theme	monosaccharides	987:1001	arg1	percentage					965:974	the mass percentage	956:974	the mass percentage of various monosaccharides	956:1001	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	0	31	theme	marine	41:46	arg1	BBL01					71:75	marine Sphingobium yanoikuyae BBL01	41:75	marine Sphingobium yanoikuyae BBL01	41:75	Bioprospecting of exopolysaccharide from marine Sphingobium yanoikuyae BBL01: Production, characterization, and metal chelation activity.
33445012	1	32	attach	isolated	218:225	arg2	exopolysaccharide					163:179	an exopolysaccharide	160:179	an exopolysaccharide (EPS)-producing bacterial strain	160:212	In the present study, an exopolysaccharide (EPS)-producing bacterial strain was isolated from the Eastern Sea (Sokcho Beach) of South Korea and identified as Sphingobium yanoikuyae BBL01.
33445012	1	32	attach	isolated	218:225	arg2	EPS					182:184	EPS	182:184	EPS	182:184	In the present study, an exopolysaccharide (EPS)-producing bacterial strain was isolated from the Eastern Sea (Sokcho Beach) of South Korea and identified as Sphingobium yanoikuyae BBL01.
33445012	1	32	attach	isolated	218:225	arg2	BBL01					319:323	Sphingobium yanoikuyae BBL01	296:323	Sphingobium yanoikuyae BBL01	296:323	In the present study, an exopolysaccharide (EPS)-producing bacterial strain was isolated from the Eastern Sea (Sokcho Beach) of South Korea and identified as Sphingobium yanoikuyae BBL01.
33445012	1	32	attach	isolated	218:225	arg1	Sea					244:246	the Eastern Sea	232:246	the Eastern Sea (Sokcho Beach) of South Korea	232:276	In the present study, an exopolysaccharide (EPS)-producing bacterial strain was isolated from the Eastern Sea (Sokcho Beach) of South Korea and identified as Sphingobium yanoikuyae BBL01.
33445012	1	32	attach	isolated	218:225	arg1	Beach					256:260	Beach	256:260	Beach	256:260	In the present study, an exopolysaccharide (EPS)-producing bacterial strain was isolated from the Eastern Sea (Sokcho Beach) of South Korea and identified as Sphingobium yanoikuyae BBL01.
33445012	3	33	theme	Purified	439:446	arg1	EPS					448:450	Purified EPS	439:450	Purified EPS produced using lactose as the main carbon source	439:499	Purified EPS produced using lactose as the main carbon source was analyzed by GC-MS and found to be composed of α-D-xylopyranose (28.6 ± 2.0%), β-D-glucopyranose (21.0 ± 1.6%), α-D-mannopyranose (18.5 ± 1.2%), β-d-mannopyranose (13.1 ± 1.4%), β-D-xylopyranose (10.2 ± 2.1%), α-d-talopyranose (5.9 ± 1.1%), and β-d-galacturonic acid (2.43 ± 0.8%).
33445012	4	34	from	change	946:951	arg1	percentage					965:974	the mass percentage	956:974	the mass percentage of various monosaccharides	956:1001	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	2	35	theme	Media	326:330	arg1	optimization					332:343	Media optimization	326:343	Media optimization	326:343	Media optimization was performed using response surface design, and a yield of 2.63 ± 0.02 g/L EPS was achieved.
33445012	0	36	theme	yanoikuyae	60:69	arg1	BBL01					71:75	marine Sphingobium yanoikuyae BBL01	41:75	marine Sphingobium yanoikuyae BBL01	41:75	Bioprospecting of exopolysaccharide from marine Sphingobium yanoikuyae BBL01: Production, characterization, and metal chelation activity.
33445012	4	37	theme	carbon	811:816	arg1	galactose					836:844	galactose	836:844	galactose	836:844	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	37	theme	carbon	811:816	arg1	glycerol					847:854	glycerol	847:854	glycerol	847:854	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	37	theme	carbon	811:816	arg1	xylose					879:884	xylose	879:884	xylose	879:884	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	37	theme	carbon	811:816	arg1	sources					818:824	different carbon sources	801:824	different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose)	801:885	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	37	theme	carbon	811:816	arg1	glucose					827:833	glucose	827:833	glucose	827:833	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	37	theme	carbon	811:816	arg1	lactose					857:863	lactose	857:863	lactose	857:863	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	37	theme	carbon	811:816	arg1	sucrose					866:872	sucrose	866:872	sucrose	866:872	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	6	38	theme	considerable	1159:1170	arg1	scavenging					1212:1221	considerable emulsifying, flocculating, free-radical scavenging, and metal-complexion activity	1159:1252	scavenging	1212:1221	EPS also showed considerable emulsifying, flocculating, free-radical scavenging, and metal-complexion activity, suggesting various biotechnological applications.
33445012	1	39	theme	Sphingobium	296:306	arg1	exopolysaccharide					163:179	an exopolysaccharide	160:179	an exopolysaccharide (EPS)-producing bacterial strain	160:212	In the present study, an exopolysaccharide (EPS)-producing bacterial strain was isolated from the Eastern Sea (Sokcho Beach) of South Korea and identified as Sphingobium yanoikuyae BBL01.
33445012	1	39	theme	Sphingobium	296:306	arg1	BBL01					319:323	Sphingobium yanoikuyae BBL01	296:323	Sphingobium yanoikuyae BBL01	296:323	In the present study, an exopolysaccharide (EPS)-producing bacterial strain was isolated from the Eastern Sea (Sokcho Beach) of South Korea and identified as Sphingobium yanoikuyae BBL01.
33445012	6	40	dep	emulsifying	1172:1182	arg1	free-radical					1199:1210	free-radical	1199:1210	free-radical	1199:1210	EPS also showed considerable emulsifying, flocculating, free-radical scavenging, and metal-complexion activity, suggesting various biotechnological applications.
33445012	6	40	dep	emulsifying	1172:1182	arg1	flocculating					1185:1196	flocculating	1185:1196	flocculating	1185:1196	EPS also showed considerable emulsifying, flocculating, free-radical scavenging, and metal-complexion activity, suggesting various biotechnological applications.
33445012	5	41	theme	gelling	1098:1104	arg1	agent					1106:1110	a thickening and gelling agent	1081:1110	a thickening and gelling agent for food-related applications	1081:1140	Purified EPS was stable up to 233 °C, indicating its possible suitability as a thickening and gelling agent for food-related applications.
33445012	5	41	theme	gelling	1098:1104	arg1	suitability					1066:1076	its possible suitability	1053:1076	its possible suitability	1053:1076	Purified EPS was stable up to 233 °C, indicating its possible suitability as a thickening and gelling agent for food-related applications.
33445012	1	42	theme	present	145:151	arg1	study					153:157	the present study	141:157	the present study	141:157	In the present study, an exopolysaccharide (EPS)-producing bacterial strain was isolated from the Eastern Sea (Sokcho Beach) of South Korea and identified as Sphingobium yanoikuyae BBL01.
33445012	4	43	theme	mass	960:963	arg1	percentage					965:974	the mass percentage	956:974	the mass percentage of various monosaccharides	956:1001	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	4	44	from	effect	897:902	arg1	composition					919:929	EPS monomer composition	907:929	EPS monomer composition	907:929	Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides.
33445012	0	45	dep	Bioprospecting	0:13	arg1	activity					128:135	metal chelation activity	112:135	metal chelation activity	112:135	Bioprospecting of exopolysaccharide from marine Sphingobium yanoikuyae BBL01: Production, characterization, and metal chelation activity.
33445012	0	45	dep	Bioprospecting	0:13	arg1	characterization					90:105	characterization	90:105	characterization	90:105	Bioprospecting of exopolysaccharide from marine Sphingobium yanoikuyae BBL01: Production, characterization, and metal chelation activity.
33445012	0	45	dep	Bioprospecting	0:13	arg1	Production					78:87	Production	78:87	Production	78:87	Bioprospecting of exopolysaccharide from marine Sphingobium yanoikuyae BBL01: Production, characterization, and metal chelation activity.
33445012	1	46	theme	Eastern	236:242	arg1	Sea					244:246	the Eastern Sea	232:246	the Eastern Sea (Sokcho Beach) of South Korea	232:276	In the present study, an exopolysaccharide (EPS)-producing bacterial strain was isolated from the Eastern Sea (Sokcho Beach) of South Korea and identified as Sphingobium yanoikuyae BBL01.
33445012	1	46	theme	Eastern	236:242	arg1	Beach					256:260	Beach	256:260	Beach	256:260	In the present study, an exopolysaccharide (EPS)-producing bacterial strain was isolated from the Eastern Sea (Sokcho Beach) of South Korea and identified as Sphingobium yanoikuyae BBL01.
32220642	0	0	theme	polysaccharide	78:91	arg1	multilayers					93:103	heparin-mimetic polysaccharide multilayers	62:103	heparin-mimetic polysaccharide multilayers	62:103	Preparation of multifunctional poly(l-lactic acid) film using heparin-mimetic polysaccharide multilayers: Hemocompatibility, cytotoxicity, antibacterial and drug loading/releasing properties.
32220642	6	1	theme	releasing	1302:1310	arg1	behavior					1312:1319	drug loading/sustained releasing behavior	1279:1319	drug loading/sustained releasing behavior	1279:1319	Moreover, the multilayer-modified PLLA exhibited non-cytotoxicity, good antibacterial ability against E. coli and S. aureus, and drug loading/sustained releasing behavior.
32220642	4	2	theme	UV-vis	877:882	arg1	spectroscopy					884:895	UV-vis spectroscopy	877:895	UV-vis spectroscopy	877:895	The surface chemical compositions, morphologies and growth manner of SCYP/CS multilayers were investigated using X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy and UV-vis spectroscopy.
32220642	4	3	theme	surface	676:682	arg1	compositions					693:704	The surface chemical compositions	672:704	The surface chemical compositions	672:704	The surface chemical compositions, morphologies and growth manner of SCYP/CS multilayers were investigated using X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy and UV-vis spectroscopy.
32220642	7	4	theme	antibacterial	1428:1440	arg1	properties					1377:1386	integrated properties	1366:1386	integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior	1366:1476	Overall, the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior could be successfully achieved by deposition of SCYP/CS multilayers, which will have potential application in blood-contacting biomedical materials.
32220642	0	5	theme	heparin-mimetic	62:76	arg1	multilayers					93:103	heparin-mimetic polysaccharide multilayers	62:103	heparin-mimetic polysaccharide multilayers	62:103	Preparation of multifunctional poly(l-lactic acid) film using heparin-mimetic polysaccharide multilayers: Hemocompatibility, cytotoxicity, antibacterial and drug loading/releasing properties.
32220642	5	6	theme	complement	1094:1103	arg1	activation					1105:1114	complement activation	1094:1114	complement activation	1094:1114	The relevant hemocompatibility results showed that multilayer-modified PLLA could effectively resist protein adsorption, suppress the platelet adhesion, prolong clotting time, prevent contact and complement activation as well as reduce hemolysis rate.
32220642	5	7	theme	hemocompatibility	911:927	arg1	results					929:935	The relevant hemocompatibility results	898:935	The relevant hemocompatibility results	898:935	The relevant hemocompatibility results showed that multilayer-modified PLLA could effectively resist protein adsorption, suppress the platelet adhesion, prolong clotting time, prevent contact and complement activation as well as reduce hemolysis rate.
32220642	6	8	theme	multilayer-modified	1164:1182	arg1	PLLA					1184:1187	the multilayer-modified PLLA	1160:1187	the multilayer-modified PLLA	1160:1187	Moreover, the multilayer-modified PLLA exhibited non-cytotoxicity, good antibacterial ability against E. coli and S. aureus, and drug loading/sustained releasing behavior.
32220642	1	9	theme	used	246:249	arg1	Poly					192:195	Poly	192:195	Poly(l-lactic acid) (PLLA)	192:217	Poly(l-lactic acid) (PLLA) has been the most commonly used polymer for making bioresorbable vascular scaffolds (BVS).
32220642	1	9	theme	used	246:249	arg1	polymer					251:257	the most commonly used polymer	228:257	the most commonly used polymer for making bioresorbable vascular scaffolds (BVS)	228:307	Poly(l-lactic acid) (PLLA) has been the most commonly used polymer for making bioresorbable vascular scaffolds (BVS).
32220642	7	10	theme	drug	1446:1449	arg1	behavior					1469:1476	drug loading/releasing behavior	1446:1476	drug loading/releasing behavior	1446:1476	Overall, the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior could be successfully achieved by deposition of SCYP/CS multilayers, which will have potential application in blood-contacting biomedical materials.
32220642	5	11	theme	clotting	1059:1066	arg1	time					1068:1071	clotting time	1059:1071	clotting time	1059:1071	The relevant hemocompatibility results showed that multilayer-modified PLLA could effectively resist protein adsorption, suppress the platelet adhesion, prolong clotting time, prevent contact and complement activation as well as reduce hemolysis rate.
32220642	7	12	theme	loading/releasing	1451:1467	arg1	behavior					1469:1476	drug loading/releasing behavior	1446:1476	drug loading/releasing behavior	1446:1476	Overall, the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior could be successfully achieved by deposition of SCYP/CS multilayers, which will have potential application in blood-contacting biomedical materials.
32220642	3	13	theme	heparin-mimetic	515:529	arg1	multilayers					546:556	heparin-mimetic polysaccharide multilayers	515:556	heparin-mimetic polysaccharide multilayers consisting of sulfated Chinese yam polysaccharide (SCYP) and chitosan (CS) through layer-by-layer (LBL) assembly	515:669	To solve this issue, we modified the PLLA film surface with heparin-mimetic polysaccharide multilayers consisting of sulfated Chinese yam polysaccharide (SCYP) and chitosan (CS) through layer-by-layer (LBL) assembly.
32220642	6	14	theme	loading/sustained	1284:1300	arg1	behavior					1312:1319	drug loading/sustained releasing behavior	1279:1319	drug loading/sustained releasing behavior	1279:1319	Moreover, the multilayer-modified PLLA exhibited non-cytotoxicity, good antibacterial ability against E. coli and S. aureus, and drug loading/sustained releasing behavior.
32220642	6	15	theme	good	1217:1220	arg1	ability					1236:1242	good antibacterial ability	1217:1242	good antibacterial ability against E. coli and S. aureus	1217:1272	Moreover, the multilayer-modified PLLA exhibited non-cytotoxicity, good antibacterial ability against E. coli and S. aureus, and drug loading/sustained releasing behavior.
32220642	5	16	theme	hemolysis	1134:1142	arg1	rate					1144:1147	hemolysis rate	1134:1147	hemolysis rate	1134:1147	The relevant hemocompatibility results showed that multilayer-modified PLLA could effectively resist protein adsorption, suppress the platelet adhesion, prolong clotting time, prevent contact and complement activation as well as reduce hemolysis rate.
32220642	4	17	theme	SCYP/CS	741:747	arg1	multilayers					749:759	SCYP/CS multilayers	741:759	SCYP/CS multilayers	741:759	The surface chemical compositions, morphologies and growth manner of SCYP/CS multilayers were investigated using X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy and UV-vis spectroscopy.
32220642	3	18	theme	polysaccharide	531:544	arg1	multilayers					546:556	heparin-mimetic polysaccharide multilayers	515:556	heparin-mimetic polysaccharide multilayers consisting of sulfated Chinese yam polysaccharide (SCYP) and chitosan (CS) through layer-by-layer (LBL) assembly	515:669	To solve this issue, we modified the PLLA film surface with heparin-mimetic polysaccharide multilayers consisting of sulfated Chinese yam polysaccharide (SCYP) and chitosan (CS) through layer-by-layer (LBL) assembly.
32220642	7	19	contain	have	1558:1561	arg1	deposition					1512:1521	deposition	1512:1521	deposition	1512:1521	Overall, the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior could be successfully achieved by deposition of SCYP/CS multilayers, which will have potential application in blood-contacting biomedical materials.
32220642	7	19	contain	have	1558:1561	arg2	application					1573:1583	potential application	1563:1583	potential application	1563:1583	Overall, the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior could be successfully achieved by deposition of SCYP/CS multilayers, which will have potential application in blood-contacting biomedical materials.
32220642	7	20	theme	potential	1563:1571	arg1	application					1573:1583	potential application	1563:1583	potential application	1563:1583	Overall, the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior could be successfully achieved by deposition of SCYP/CS multilayers, which will have potential application in blood-contacting biomedical materials.
32220642	4	21	theme	atomic	849:854	arg1	microscopy					862:871	atomic force microscopy	849:871	atomic force microscopy	849:871	The surface chemical compositions, morphologies and growth manner of SCYP/CS multilayers were investigated using X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy and UV-vis spectroscopy.
32220642	2	22	theme	higher	378:383	arg1	rates					385:389	higher rates	378:389	higher rates of early thrombosis	378:409	Despite owning remarkable properties, BVS made from PLLA are facing higher rates of early thrombosis compared with permanent metallic scaffolds.
32220642	6	23	theme	antibacterial	1222:1234	arg1	ability					1236:1242	good antibacterial ability	1217:1242	good antibacterial ability against E. coli and S. aureus	1217:1272	Moreover, the multilayer-modified PLLA exhibited non-cytotoxicity, good antibacterial ability against E. coli and S. aureus, and drug loading/sustained releasing behavior.
32220642	7	24	theme	non-cytotoxicity	1410:1425	arg1	properties					1377:1386	integrated properties	1366:1386	integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior	1366:1476	Overall, the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior could be successfully achieved by deposition of SCYP/CS multilayers, which will have potential application in blood-contacting biomedical materials.
32220642	2	25	theme	metallic	435:442	arg1	scaffolds					444:452	permanent metallic scaffolds	425:452	permanent metallic scaffolds	425:452	Despite owning remarkable properties, BVS made from PLLA are facing higher rates of early thrombosis compared with permanent metallic scaffolds.
32220642	4	26	theme	growth	724:729	arg1	manner					731:736	growth manner	724:736	growth manner	724:736	The surface chemical compositions, morphologies and growth manner of SCYP/CS multilayers were investigated using X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy and UV-vis spectroscopy.
32220642	1	27	theme	l-lactic	197:204	arg1	Poly					192:195	Poly	192:195	Poly(l-lactic acid) (PLLA)	192:217	Poly(l-lactic acid) (PLLA) has been the most commonly used polymer for making bioresorbable vascular scaffolds (BVS).
32220642	1	27	theme	l-lactic	197:204	arg1	acid					206:209	l-lactic acid	197:209	l-lactic acid	197:209	Poly(l-lactic acid) (PLLA) has been the most commonly used polymer for making bioresorbable vascular scaffolds (BVS).
32220642	2	28	theme	permanent	425:433	arg1	scaffolds					444:452	permanent metallic scaffolds	425:452	permanent metallic scaffolds	425:452	Despite owning remarkable properties, BVS made from PLLA are facing higher rates of early thrombosis compared with permanent metallic scaffolds.
32220642	7	29	theme	multilayers	1534:1544	arg1	deposition					1512:1521	deposition	1512:1521	deposition	1512:1521	Overall, the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior could be successfully achieved by deposition of SCYP/CS multilayers, which will have potential application in blood-contacting biomedical materials.
32220642	4	30	theme	force	856:860	arg1	microscopy					862:871	atomic force microscopy	849:871	atomic force microscopy	849:871	The surface chemical compositions, morphologies and growth manner of SCYP/CS multilayers were investigated using X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy and UV-vis spectroscopy.
32220642	7	31	theme	biomedical	1605:1614	arg1	materials					1616:1624	blood-contacting biomedical materials	1588:1624	blood-contacting biomedical materials	1588:1624	Overall, the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior could be successfully achieved by deposition of SCYP/CS multilayers, which will have potential application in blood-contacting biomedical materials.
32220642	3	32	theme	sulfated	572:579	arg1	polysaccharide					593:606	sulfated Chinese yam polysaccharide	572:606	sulfated Chinese yam polysaccharide (SCYP)	572:613	To solve this issue, we modified the PLLA film surface with heparin-mimetic polysaccharide multilayers consisting of sulfated Chinese yam polysaccharide (SCYP) and chitosan (CS) through layer-by-layer (LBL) assembly.
32220642	3	32	theme	sulfated	572:579	arg1	SCYP					609:612	SCYP	609:612	SCYP	609:612	To solve this issue, we modified the PLLA film surface with heparin-mimetic polysaccharide multilayers consisting of sulfated Chinese yam polysaccharide (SCYP) and chitosan (CS) through layer-by-layer (LBL) assembly.
32220642	7	33	theme	hemocompatibility	1391:1407	arg1	properties					1377:1386	integrated properties	1366:1386	integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior	1366:1476	Overall, the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior could be successfully achieved by deposition of SCYP/CS multilayers, which will have potential application in blood-contacting biomedical materials.
32220642	0	34	theme	poly	31:34	arg1	film					51:54	multifunctional poly(l-lactic acid) film	15:54	multifunctional poly(l-lactic acid) film using heparin-mimetic polysaccharide multilayers	15:103	Preparation of multifunctional poly(l-lactic acid) film using heparin-mimetic polysaccharide multilayers: Hemocompatibility, cytotoxicity, antibacterial and drug loading/releasing properties.
32220642	1	35	theme	bioresorbable	270:282	arg1	BVS					304:306	BVS	304:306	BVS	304:306	Poly(l-lactic acid) (PLLA) has been the most commonly used polymer for making bioresorbable vascular scaffolds (BVS).
32220642	1	35	theme	bioresorbable	270:282	arg1	scaffolds					293:301	bioresorbable vascular scaffolds	270:301	bioresorbable vascular scaffolds (BVS)	270:307	Poly(l-lactic acid) (PLLA) has been the most commonly used polymer for making bioresorbable vascular scaffolds (BVS).
32220642	7	36	with	film	1356:1359	arg1	properties					1377:1386	integrated properties	1366:1386	integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior	1366:1476	Overall, the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior could be successfully achieved by deposition of SCYP/CS multilayers, which will have potential application in blood-contacting biomedical materials.
32220642	4	37	theme	electron	828:835	arg1	microscopy					837:846	scanning electron microscopy	819:846	scanning electron microscopy	819:846	The surface chemical compositions, morphologies and growth manner of SCYP/CS multilayers were investigated using X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy and UV-vis spectroscopy.
32220642	4	38	theme	scanning	819:826	arg1	microscopy					837:846	scanning electron microscopy	819:846	scanning electron microscopy	819:846	The surface chemical compositions, morphologies and growth manner of SCYP/CS multilayers were investigated using X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy and UV-vis spectroscopy.
32220642	2	39	theme	thrombosis	400:409	arg1	rates					385:389	higher rates	378:389	higher rates of early thrombosis	378:409	Despite owning remarkable properties, BVS made from PLLA are facing higher rates of early thrombosis compared with permanent metallic scaffolds.
32220642	0	40	theme	multifunctional	15:29	arg1	film					51:54	multifunctional poly(l-lactic acid) film	15:54	multifunctional poly(l-lactic acid) film using heparin-mimetic polysaccharide multilayers	15:103	Preparation of multifunctional poly(l-lactic acid) film using heparin-mimetic polysaccharide multilayers: Hemocompatibility, cytotoxicity, antibacterial and drug loading/releasing properties.
32220642	1	41	theme	vascular	284:291	arg1	BVS					304:306	BVS	304:306	BVS	304:306	Poly(l-lactic acid) (PLLA) has been the most commonly used polymer for making bioresorbable vascular scaffolds (BVS).
32220642	1	41	theme	vascular	284:291	arg1	scaffolds					293:301	bioresorbable vascular scaffolds	270:301	bioresorbable vascular scaffolds (BVS)	270:307	Poly(l-lactic acid) (PLLA) has been the most commonly used polymer for making bioresorbable vascular scaffolds (BVS).
32220642	5	42	theme	protein	999:1005	arg1	adsorption					1007:1016	protein adsorption	999:1016	protein adsorption	999:1016	The relevant hemocompatibility results showed that multilayer-modified PLLA could effectively resist protein adsorption, suppress the platelet adhesion, prolong clotting time, prevent contact and complement activation as well as reduce hemolysis rate.
32220642	2	43	theme	early	394:398	arg1	thrombosis					400:409	early thrombosis	394:409	early thrombosis	394:409	Despite owning remarkable properties, BVS made from PLLA are facing higher rates of early thrombosis compared with permanent metallic scaffolds.
32220642	0	44	theme	l-lactic	36:43	arg1	poly					31:34	poly	31:34	multifunctional poly(l-lactic acid) film using heparin-mimetic polysaccharide multilayers	15:103	Preparation of multifunctional poly(l-lactic acid) film using heparin-mimetic polysaccharide multilayers: Hemocompatibility, cytotoxicity, antibacterial and drug loading/releasing properties.
32220642	0	44	theme	l-lactic	36:43	arg1	acid					45:48	l-lactic acid	36:48	l-lactic acid	36:48	Preparation of multifunctional poly(l-lactic acid) film using heparin-mimetic polysaccharide multilayers: Hemocompatibility, cytotoxicity, antibacterial and drug loading/releasing properties.
32220642	4	45	theme	chemical	684:691	arg1	compositions					693:704	The surface chemical compositions	672:704	The surface chemical compositions	672:704	The surface chemical compositions, morphologies and growth manner of SCYP/CS multilayers were investigated using X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy and UV-vis spectroscopy.
32220642	4	46	theme	photoelectron	791:803	arg1	spectroscopy					805:816	X-ray photoelectron spectroscopy	785:816	X-ray photoelectron spectroscopy	785:816	The surface chemical compositions, morphologies and growth manner of SCYP/CS multilayers were investigated using X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy and UV-vis spectroscopy.
32220642	6	47	theme	drug	1279:1282	arg1	behavior					1312:1319	drug loading/sustained releasing behavior	1279:1319	drug loading/sustained releasing behavior	1279:1319	Moreover, the multilayer-modified PLLA exhibited non-cytotoxicity, good antibacterial ability against E. coli and S. aureus, and drug loading/sustained releasing behavior.
32220642	7	48	theme	behavior	1469:1476	arg1	properties					1377:1386	integrated properties	1366:1386	integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior	1366:1476	Overall, the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior could be successfully achieved by deposition of SCYP/CS multilayers, which will have potential application in blood-contacting biomedical materials.
32220642	0	49	theme	loading/releasing	162:178	arg1	properties					180:189	drug loading/releasing properties	157:189	drug loading/releasing properties	157:189	Preparation of multifunctional poly(l-lactic acid) film using heparin-mimetic polysaccharide multilayers: Hemocompatibility, cytotoxicity, antibacterial and drug loading/releasing properties.
32220642	3	50	theme	Chinese	581:587	arg1	polysaccharide					593:606	sulfated Chinese yam polysaccharide	572:606	sulfated Chinese yam polysaccharide (SCYP)	572:613	To solve this issue, we modified the PLLA film surface with heparin-mimetic polysaccharide multilayers consisting of sulfated Chinese yam polysaccharide (SCYP) and chitosan (CS) through layer-by-layer (LBL) assembly.
32220642	3	50	theme	Chinese	581:587	arg1	SCYP					609:612	SCYP	609:612	SCYP	609:612	To solve this issue, we modified the PLLA film surface with heparin-mimetic polysaccharide multilayers consisting of sulfated Chinese yam polysaccharide (SCYP) and chitosan (CS) through layer-by-layer (LBL) assembly.
32220642	7	51	theme	multifunctional	1335:1349	arg1	film					1356:1359	the multifunctional PLLA film	1331:1359	the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior	1331:1476	Overall, the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior could be successfully achieved by deposition of SCYP/CS multilayers, which will have potential application in blood-contacting biomedical materials.
32220642	0	52	theme	drug	157:160	arg1	properties					180:189	drug loading/releasing properties	157:189	drug loading/releasing properties	157:189	Preparation of multifunctional poly(l-lactic acid) film using heparin-mimetic polysaccharide multilayers: Hemocompatibility, cytotoxicity, antibacterial and drug loading/releasing properties.
32220642	3	53	theme	LBL	657:659	arg1	assembly					662:669	layer-by-layer (LBL) assembly	641:669	layer-by-layer (LBL) assembly	641:669	To solve this issue, we modified the PLLA film surface with heparin-mimetic polysaccharide multilayers consisting of sulfated Chinese yam polysaccharide (SCYP) and chitosan (CS) through layer-by-layer (LBL) assembly.
32220642	3	54	theme	yam	589:591	arg1	polysaccharide					593:606	sulfated Chinese yam polysaccharide	572:606	sulfated Chinese yam polysaccharide (SCYP)	572:613	To solve this issue, we modified the PLLA film surface with heparin-mimetic polysaccharide multilayers consisting of sulfated Chinese yam polysaccharide (SCYP) and chitosan (CS) through layer-by-layer (LBL) assembly.
32220642	3	54	theme	yam	589:591	arg1	SCYP					609:612	SCYP	609:612	SCYP	609:612	To solve this issue, we modified the PLLA film surface with heparin-mimetic polysaccharide multilayers consisting of sulfated Chinese yam polysaccharide (SCYP) and chitosan (CS) through layer-by-layer (LBL) assembly.
32220642	2	55	theme	remarkable	325:334	arg1	properties					336:345	remarkable properties	325:345	remarkable properties	325:345	Despite owning remarkable properties, BVS made from PLLA are facing higher rates of early thrombosis compared with permanent metallic scaffolds.
32220642	0	56	dep	Preparation	0:10	arg1	antibacterial					139:151	antibacterial	139:151	antibacterial	139:151	Preparation of multifunctional poly(l-lactic acid) film using heparin-mimetic polysaccharide multilayers: Hemocompatibility, cytotoxicity, antibacterial and drug loading/releasing properties.
32220642	0	56	dep	Preparation	0:10	arg1	Hemocompatibility					106:122	Hemocompatibility	106:122	Hemocompatibility	106:122	Preparation of multifunctional poly(l-lactic acid) film using heparin-mimetic polysaccharide multilayers: Hemocompatibility, cytotoxicity, antibacterial and drug loading/releasing properties.
32220642	0	56	dep	Preparation	0:10	arg1	cytotoxicity					125:136	cytotoxicity	125:136	cytotoxicity	125:136	Preparation of multifunctional poly(l-lactic acid) film using heparin-mimetic polysaccharide multilayers: Hemocompatibility, cytotoxicity, antibacterial and drug loading/releasing properties.
32220642	0	56	dep	Preparation	0:10	arg1	properties					180:189	drug loading/releasing properties	157:189	drug loading/releasing properties	157:189	Preparation of multifunctional poly(l-lactic acid) film using heparin-mimetic polysaccharide multilayers: Hemocompatibility, cytotoxicity, antibacterial and drug loading/releasing properties.
32220642	5	57	theme	platelet	1032:1039	arg1	adhesion					1041:1048	the platelet adhesion	1028:1048	the platelet adhesion	1028:1048	The relevant hemocompatibility results showed that multilayer-modified PLLA could effectively resist protein adsorption, suppress the platelet adhesion, prolong clotting time, prevent contact and complement activation as well as reduce hemolysis rate.
32220642	4	58	theme	X-ray	785:789	arg1	spectroscopy					805:816	X-ray photoelectron spectroscopy	785:816	X-ray photoelectron spectroscopy	785:816	The surface chemical compositions, morphologies and growth manner of SCYP/CS multilayers were investigated using X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy and UV-vis spectroscopy.
32220642	5	59	theme	multilayer-modified	949:967	arg1	PLLA					969:972	multilayer-modified PLLA	949:972	multilayer-modified PLLA	949:972	The relevant hemocompatibility results showed that multilayer-modified PLLA could effectively resist protein adsorption, suppress the platelet adhesion, prolong clotting time, prevent contact and complement activation as well as reduce hemolysis rate.
32220642	7	60	theme	SCYP/CS	1526:1532	arg1	multilayers					1534:1544	SCYP/CS multilayers	1526:1544	SCYP/CS multilayers	1526:1544	Overall, the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior could be successfully achieved by deposition of SCYP/CS multilayers, which will have potential application in blood-contacting biomedical materials.
32220642	3	61	theme	layer-by-layer	641:654	arg1	assembly					662:669	layer-by-layer (LBL) assembly	641:669	layer-by-layer (LBL) assembly	641:669	To solve this issue, we modified the PLLA film surface with heparin-mimetic polysaccharide multilayers consisting of sulfated Chinese yam polysaccharide (SCYP) and chitosan (CS) through layer-by-layer (LBL) assembly.
32220642	4	62	theme	multilayers	749:759	arg1	manner					731:736	growth manner	724:736	growth manner	724:736	The surface chemical compositions, morphologies and growth manner of SCYP/CS multilayers were investigated using X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy and UV-vis spectroscopy.
32220642	4	62	theme	multilayers	749:759	arg1	compositions					693:704	The surface chemical compositions	672:704	The surface chemical compositions	672:704	The surface chemical compositions, morphologies and growth manner of SCYP/CS multilayers were investigated using X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy and UV-vis spectroscopy.
32220642	4	62	theme	multilayers	749:759	arg1	morphologies					707:718	morphologies	707:718	morphologies	707:718	The surface chemical compositions, morphologies and growth manner of SCYP/CS multilayers were investigated using X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy and UV-vis spectroscopy.
32220642	5	63	theme	relevant	902:909	arg1	results					929:935	The relevant hemocompatibility results	898:935	The relevant hemocompatibility results	898:935	The relevant hemocompatibility results showed that multilayer-modified PLLA could effectively resist protein adsorption, suppress the platelet adhesion, prolong clotting time, prevent contact and complement activation as well as reduce hemolysis rate.
32220642	7	64	theme	integrated	1366:1375	arg1	properties					1377:1386	integrated properties	1366:1386	integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior	1366:1476	Overall, the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior could be successfully achieved by deposition of SCYP/CS multilayers, which will have potential application in blood-contacting biomedical materials.
32220642	3	65	theme	PLLA	492:495	arg1	surface					502:508	the PLLA film surface	488:508	the PLLA film surface	488:508	To solve this issue, we modified the PLLA film surface with heparin-mimetic polysaccharide multilayers consisting of sulfated Chinese yam polysaccharide (SCYP) and chitosan (CS) through layer-by-layer (LBL) assembly.
32220642	0	66	theme	film	51:54	arg1	Preparation					0:10	Preparation	0:10	Preparation of multifunctional poly(l-lactic acid) film using heparin-mimetic polysaccharide multilayers: Hemocompatibility, cytotoxicity, antibacterial and drug loading/releasing properties.	0:190	Preparation of multifunctional poly(l-lactic acid) film using heparin-mimetic polysaccharide multilayers: Hemocompatibility, cytotoxicity, antibacterial and drug loading/releasing properties.
32220642	7	67	theme	blood-contacting	1588:1603	arg1	materials					1616:1624	blood-contacting biomedical materials	1588:1624	blood-contacting biomedical materials	1588:1624	Overall, the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior could be successfully achieved by deposition of SCYP/CS multilayers, which will have potential application in blood-contacting biomedical materials.
32220642	2	68	dep	facing	371:376	arg1	compared					411:418	compared	411:418	compared with permanent metallic scaffolds	411:452	Despite owning remarkable properties, BVS made from PLLA are facing higher rates of early thrombosis compared with permanent metallic scaffolds.
32220642	3	69	theme	film	497:500	arg1	surface					502:508	the PLLA film surface	488:508	the PLLA film surface	488:508	To solve this issue, we modified the PLLA film surface with heparin-mimetic polysaccharide multilayers consisting of sulfated Chinese yam polysaccharide (SCYP) and chitosan (CS) through layer-by-layer (LBL) assembly.
32220642	7	70	theme	PLLA	1351:1354	arg1	film					1356:1359	the multifunctional PLLA film	1331:1359	the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior	1331:1476	Overall, the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior could be successfully achieved by deposition of SCYP/CS multilayers, which will have potential application in blood-contacting biomedical materials.
31855431	5	0	theme	acids	860:864	arg1	metabolism					797:806	the metabolism	793:806	the metabolism of the cecal total bile acids and short-chain fatty acids	793:864	CPP could improve plasma and liver lipid metabolism and accelerate the metabolism of the cecal total bile acids and short-chain fatty acids.
31855431	6	1	theme	β-hydroxy	1043:1051	arg1	β-methylglutaryl-CoA					1053:1072	β-hydroxy β-methylglutaryl-CoA	1043:1072	β-hydroxy β-methylglutaryl-CoA	1043:1072	CPP has also upregulated the adenosine-monophosphate-activated protein kinase α and downregulated the acetyl-CoA carboxylase, sterol regulatory element-binding protein 1c, and β-hydroxy β-methylglutaryl-CoA expressions.
31855431	4	2	from	effect	594:599	arg1	rats					656:659	diet-induced rats	643:659	diet-induced rats	643:659	The hypolipidemic effect and intestinal flora regulation of CPP on diet-induced rats were evaluated through histopathology and biochemistry analyses.
31855431	1	3	theme	bioactive	168:176	arg1	polysaccharide					178:191	A bioactive polysaccharide	166:191	A bioactive polysaccharide from microalga Chlorella pyrenoidosa (CPP)	166:234	A bioactive polysaccharide from microalga Chlorella pyrenoidosa (CPP) was successively prepared via DEAE-52 and G-100 columns.
31855431	7	4	theme	intestinal	1172:1181	arg1	flora					1183:1187	intestinal flora	1172:1187	intestinal flora	1172:1187	Moreover, with the 16S rRNA gene sequencing, it was revealed that the composition of intestinal flora changed drastically after treatment, such as the bloom of Coprococcus_1, Lactobacillus, and Turicibacter, whereas there was a strong reduction of the [Ruminococcus]_gauvreauii_group.
31855431	7	5	theme	[Ruminococcus	1339:1351	arg1	_gauvreauii_group					1353:1369	the [Ruminococcus]_gauvreauii_group	1335:1369	the [Ruminococcus]_gauvreauii_group	1335:1369	Moreover, with the 16S rRNA gene sequencing, it was revealed that the composition of intestinal flora changed drastically after treatment, such as the bloom of Coprococcus_1, Lactobacillus, and Turicibacter, whereas there was a strong reduction of the [Ruminococcus]_gauvreauii_group.
31855431	0	6	from	Characterization	16:31	arg1	Activity					118:125	Its Hypolipidemic Activity	100:125	Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats	100:163	Physicochemical Characterization of a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats.
31855431	0	6	from	Characterization	16:31	arg1	pyrenoidosa					84:94	Green Microalga Chlorella pyrenoidosa	58:94	Green Microalga Chlorella pyrenoidosa	58:94	Physicochemical Characterization of a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats.
31855431	6	7	theme	kinase	938:943	arg1	α					945:945	the adenosine-monophosphate-activated protein kinase α	892:945	the adenosine-monophosphate-activated protein kinase α	892:945	CPP has also upregulated the adenosine-monophosphate-activated protein kinase α and downregulated the acetyl-CoA carboxylase, sterol regulatory element-binding protein 1c, and β-hydroxy β-methylglutaryl-CoA expressions.
31855431	6	8	theme	acetyl-CoA	969:978	arg1	carboxylase					980:990	acetyl-CoA carboxylase	969:990	acetyl-CoA carboxylase	969:990	CPP has also upregulated the adenosine-monophosphate-activated protein kinase α and downregulated the acetyl-CoA carboxylase, sterol regulatory element-binding protein 1c, and β-hydroxy β-methylglutaryl-CoA expressions.
31855431	8	9	from	flora	1510:1514	arg1	rats					1534:1537	hyperlipidemia rats	1519:1537	hyperlipidemia rats	1519:1537	The above results illustrated that CPP might be served as an effective ingredient to ameliorate lipid metabolism disorders and intestinal flora in hyperlipidemia rats.
31855431	1	10	theme	G-100	278:282	arg1	columns					284:290	G-100 columns	278:290	G-100 columns	278:290	A bioactive polysaccharide from microalga Chlorella pyrenoidosa (CPP) was successively prepared via DEAE-52 and G-100 columns.
31855431	5	11	theme	total	821:825	arg1	acids					832:836	total bile acids	821:836	total bile acids	821:836	CPP could improve plasma and liver lipid metabolism and accelerate the metabolism of the cecal total bile acids and short-chain fatty acids.
31855431	4	12	theme	hypolipidemic	580:592	arg1	effect					594:599	hypolipidemic effect	580:599	hypolipidemic effect	580:599	The hypolipidemic effect and intestinal flora regulation of CPP on diet-induced rats were evaluated through histopathology and biochemistry analyses.
31855431	5	13	theme	liver	755:759	arg1	metabolism					767:776	plasma and liver lipid metabolism	744:776	plasma and liver lipid metabolism	744:776	CPP could improve plasma and liver lipid metabolism and accelerate the metabolism of the cecal total bile acids and short-chain fatty acids.
31855431	6	14	theme	protein	930:936	arg1	kinase					938:943	the adenosine-monophosphate-activated protein kinase	892:943	the adenosine-monophosphate-activated protein kinase α	892:945	CPP has also upregulated the adenosine-monophosphate-activated protein kinase α and downregulated the acetyl-CoA carboxylase, sterol regulatory element-binding protein 1c, and β-hydroxy β-methylglutaryl-CoA expressions.
31855431	0	15	theme	Hypolipidemic	104:116	arg1	Activity					118:125	Its Hypolipidemic Activity	100:125	Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats	100:163	Physicochemical Characterization of a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats.
31855431	5	16	theme	lipid	761:765	arg1	metabolism					767:776	plasma and liver lipid metabolism	744:776	plasma and liver lipid metabolism	744:776	CPP could improve plasma and liver lipid metabolism and accelerate the metabolism of the cecal total bile acids and short-chain fatty acids.
31855431	6	17	theme	adenosine-monophosphate-activated	896:928	arg1	kinase					938:943	the adenosine-monophosphate-activated protein kinase	892:943	the adenosine-monophosphate-activated protein kinase α	892:945	CPP has also upregulated the adenosine-monophosphate-activated protein kinase α and downregulated the acetyl-CoA carboxylase, sterol regulatory element-binding protein 1c, and β-hydroxy β-methylglutaryl-CoA expressions.
31855431	2	18	theme	resonance	310:318	arg1	analysis					320:327	Nuclear magnetic resonance analysis	293:327	Nuclear magnetic resonance analysis	293:327	Nuclear magnetic resonance analysis showed that the main glycosidic bonds were composed of 1,2-linked-α-l-Fucp, 1,4-linked-α-l-Rhap, 1,4-linked-β-l-Araf, 1-linked-α-d-Glcp, 1,3-linked-β-d-GlcpA, 1,4-linked-β-d-Xylp, and 1,3,6-linked-β-d-Manp.
31855431	3	19	theme	×	566:566	arg1	Da					572:573	5.63 × 106 Da	561:573	5.63 × 106 Da	561:573	Its molecular weight was 5.63 × 106 Da.
31855431	0	20	theme	Gut	131:133	arg1	Regulation					146:155	Gut Microbiota Regulation	131:155	Gut Microbiota Regulation	131:155	Physicochemical Characterization of a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats.
31855431	6	21	theme	protein	1027:1033	arg1	expressions					1074:1084	the acetyl-CoA carboxylase, sterol regulatory element-binding protein 1c, and β-hydroxy β-methylglutaryl-CoA expressions	965:1084	the acetyl-CoA carboxylase, sterol regulatory element-binding protein 1c, and β-hydroxy β-methylglutaryl-CoA expressions	965:1084	CPP has also upregulated the adenosine-monophosphate-activated protein kinase α and downregulated the acetyl-CoA carboxylase, sterol regulatory element-binding protein 1c, and β-hydroxy β-methylglutaryl-CoA expressions.
31855431	2	22	theme	magnetic	301:308	arg1	resonance					310:318	Nuclear magnetic resonance	293:318	Nuclear magnetic resonance analysis	293:327	Nuclear magnetic resonance analysis showed that the main glycosidic bonds were composed of 1,2-linked-α-l-Fucp, 1,4-linked-α-l-Rhap, 1,4-linked-β-l-Araf, 1-linked-α-d-Glcp, 1,3-linked-β-d-GlcpA, 1,4-linked-β-d-Xylp, and 1,3,6-linked-β-d-Manp.
31855431	8	23	from	metabolism	1474:1483	arg1	rats					1534:1537	hyperlipidemia rats	1519:1537	hyperlipidemia rats	1519:1537	The above results illustrated that CPP might be served as an effective ingredient to ameliorate lipid metabolism disorders and intestinal flora in hyperlipidemia rats.
31855431	7	24	theme	strong	1315:1320	arg1	reduction					1322:1330	a strong reduction	1313:1330	a strong reduction of the [Ruminococcus]_gauvreauii_group	1313:1369	Moreover, with the 16S rRNA gene sequencing, it was revealed that the composition of intestinal flora changed drastically after treatment, such as the bloom of Coprococcus_1, Lactobacillus, and Turicibacter, whereas there was a strong reduction of the [Ruminococcus]_gauvreauii_group.
31855431	6	25	theme	element-binding	1011:1025	arg1	protein					1027:1033	sterol regulatory element-binding protein 1c	993:1036	sterol regulatory element-binding protein 1c	993:1036	CPP has also upregulated the adenosine-monophosphate-activated protein kinase α and downregulated the acetyl-CoA carboxylase, sterol regulatory element-binding protein 1c, and β-hydroxy β-methylglutaryl-CoA expressions.
31855431	2	26	theme	Nuclear	293:299	arg1	resonance					310:318	Nuclear magnetic resonance	293:318	Nuclear magnetic resonance analysis	293:327	Nuclear magnetic resonance analysis showed that the main glycosidic bonds were composed of 1,2-linked-α-l-Fucp, 1,4-linked-α-l-Rhap, 1,4-linked-β-l-Araf, 1-linked-α-d-Glcp, 1,3-linked-β-d-GlcpA, 1,4-linked-β-d-Xylp, and 1,3,6-linked-β-d-Manp.
31855431	1	27	theme	microalga	198:206	arg1	CPP					231:233	CPP	231:233	CPP	231:233	A bioactive polysaccharide from microalga Chlorella pyrenoidosa (CPP) was successively prepared via DEAE-52 and G-100 columns.
31855431	1	27	theme	microalga	198:206	arg1	pyrenoidosa					218:228	microalga Chlorella pyrenoidosa	198:228	microalga Chlorella pyrenoidosa (CPP)	198:234	A bioactive polysaccharide from microalga Chlorella pyrenoidosa (CPP) was successively prepared via DEAE-52 and G-100 columns.
31855431	5	28	theme	plasma	744:749	arg1	metabolism					767:776	plasma and liver lipid metabolism	744:776	plasma and liver lipid metabolism	744:776	CPP could improve plasma and liver lipid metabolism and accelerate the metabolism of the cecal total bile acids and short-chain fatty acids.
31855431	6	29	theme	regulatory	1000:1009	arg1	protein					1027:1033	sterol regulatory element-binding protein 1c	993:1036	sterol regulatory element-binding protein 1c	993:1036	CPP has also upregulated the adenosine-monophosphate-activated protein kinase α and downregulated the acetyl-CoA carboxylase, sterol regulatory element-binding protein 1c, and β-hydroxy β-methylglutaryl-CoA expressions.
31855431	2	30	theme	main	345:348	arg1	bonds					361:365	the main glycosidic bonds	341:365	the main glycosidic bonds	341:365	Nuclear magnetic resonance analysis showed that the main glycosidic bonds were composed of 1,2-linked-α-l-Fucp, 1,4-linked-α-l-Rhap, 1,4-linked-β-l-Araf, 1-linked-α-d-Glcp, 1,3-linked-β-d-GlcpA, 1,4-linked-β-d-Xylp, and 1,3,6-linked-β-d-Manp.
31855431	1	31	theme	Chlorella	208:216	arg1	CPP					231:233	CPP	231:233	CPP	231:233	A bioactive polysaccharide from microalga Chlorella pyrenoidosa (CPP) was successively prepared via DEAE-52 and G-100 columns.
31855431	1	31	theme	Chlorella	208:216	arg1	pyrenoidosa					218:228	microalga Chlorella pyrenoidosa	198:228	microalga Chlorella pyrenoidosa (CPP)	198:234	A bioactive polysaccharide from microalga Chlorella pyrenoidosa (CPP) was successively prepared via DEAE-52 and G-100 columns.
31855431	0	32	theme	Physicochemical	0:14	arg1	Characterization					16:31	Physicochemical Characterization	0:31	Physicochemical Characterization of a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats.	0:164	Physicochemical Characterization of a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats.
31855431	4	33	theme	CPP	636:638	arg1	flora					616:620	intestinal flora	605:620	intestinal flora	605:620	The hypolipidemic effect and intestinal flora regulation of CPP on diet-induced rats were evaluated through histopathology and biochemistry analyses.
31855431	4	33	theme	CPP	636:638	arg1	effect					594:599	hypolipidemic effect	580:599	hypolipidemic effect	580:599	The hypolipidemic effect and intestinal flora regulation of CPP on diet-induced rats were evaluated through histopathology and biochemistry analyses.
31855431	8	34	theme	intestinal	1499:1508	arg1	flora					1510:1514	intestinal flora	1499:1514	intestinal flora in hyperlipidemia rats	1499:1537	The above results illustrated that CPP might be served as an effective ingredient to ameliorate lipid metabolism disorders and intestinal flora in hyperlipidemia rats.
31855431	8	35	theme	above	1376:1380	arg1	results					1382:1388	The above results	1372:1388	The above results	1372:1388	The above results illustrated that CPP might be served as an effective ingredient to ameliorate lipid metabolism disorders and intestinal flora in hyperlipidemia rats.
31855431	8	36	theme	hyperlipidemia	1519:1532	arg1	rats					1534:1537	hyperlipidemia rats	1519:1537	hyperlipidemia rats	1519:1537	The above results illustrated that CPP might be served as an effective ingredient to ameliorate lipid metabolism disorders and intestinal flora in hyperlipidemia rats.
31855431	7	37	theme	16S	1106:1108	arg1	sequencing					1120:1129	the 16S rRNA gene sequencing	1102:1129	the 16S rRNA gene sequencing	1102:1129	Moreover, with the 16S rRNA gene sequencing, it was revealed that the composition of intestinal flora changed drastically after treatment, such as the bloom of Coprococcus_1, Lactobacillus, and Turicibacter, whereas there was a strong reduction of the [Ruminococcus]_gauvreauii_group.
31855431	4	38	theme	diet-induced	643:654	arg1	rats					656:659	diet-induced rats	643:659	diet-induced rats	643:659	The hypolipidemic effect and intestinal flora regulation of CPP on diet-induced rats were evaluated through histopathology and biochemistry analyses.
31855431	0	39	theme	Microbiota	135:144	arg1	Regulation					146:155	Gut Microbiota Regulation	131:155	Gut Microbiota Regulation	131:155	Physicochemical Characterization of a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats.
31855431	6	40	theme	sterol	993:998	arg1	protein					1027:1033	sterol regulatory element-binding protein 1c	993:1036	sterol regulatory element-binding protein 1c	993:1036	CPP has also upregulated the adenosine-monophosphate-activated protein kinase α and downregulated the acetyl-CoA carboxylase, sterol regulatory element-binding protein 1c, and β-hydroxy β-methylglutaryl-CoA expressions.
31855431	5	41	theme	cecal	815:819	arg1	acids					832:836	total bile acids	821:836	total bile acids	821:836	CPP could improve plasma and liver lipid metabolism and accelerate the metabolism of the cecal total bile acids and short-chain fatty acids.
31855431	8	42	dep	metabolism	1474:1483	arg1	disorders					1485:1493	disorders	1485:1493	disorders	1485:1493	The above results illustrated that CPP might be served as an effective ingredient to ameliorate lipid metabolism disorders and intestinal flora in hyperlipidemia rats.
31855431	0	43	theme	Polysaccharide	38:51	arg1	Characterization					16:31	Physicochemical Characterization	0:31	Physicochemical Characterization of a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats.	0:164	Physicochemical Characterization of a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats.
31855431	4	44	theme	intestinal	605:614	arg1	flora					616:620	intestinal flora	605:620	intestinal flora	605:620	The hypolipidemic effect and intestinal flora regulation of CPP on diet-induced rats were evaluated through histopathology and biochemistry analyses.
31855431	4	45	from	flora	616:620	arg1	rats					656:659	diet-induced rats	643:659	diet-induced rats	643:659	The hypolipidemic effect and intestinal flora regulation of CPP on diet-induced rats were evaluated through histopathology and biochemistry analyses.
31855431	0	46	from	pyrenoidosa	84:94	arg1	Rats					160:163	Rats	160:163	Rats	160:163	Physicochemical Characterization of a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats.
31855431	0	46	from	pyrenoidosa	84:94	arg1	Characterization					16:31	Physicochemical Characterization	0:31	Physicochemical Characterization of a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats.	0:164	Physicochemical Characterization of a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats.
31855431	0	46	from	pyrenoidosa	84:94	arg1	Polysaccharide					38:51	a Polysaccharide	36:51	a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats	36:163	Physicochemical Characterization of a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats.
31855431	3	47	theme	molecular	540:548	arg1	weight					550:555	Its molecular weight	536:555	Its molecular weight	536:555	Its molecular weight was 5.63 × 106 Da.
31855431	0	48	theme	Microalga	64:72	arg1	pyrenoidosa					84:94	Green Microalga Chlorella pyrenoidosa	58:94	Green Microalga Chlorella pyrenoidosa	58:94	Physicochemical Characterization of a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats.
31855431	4	49	dep	effect	594:599	arg1	The					576:578	The	576:578	The	576:578	The hypolipidemic effect and intestinal flora regulation of CPP on diet-induced rats were evaluated through histopathology and biochemistry analyses.
31855431	4	49	dep	effect	594:599	arg1	regulation					622:631	regulation	622:631	regulation	622:631	The hypolipidemic effect and intestinal flora regulation of CPP on diet-induced rats were evaluated through histopathology and biochemistry analyses.
31855431	5	50	dep	acids	832:836	arg1	the					811:813	the	811:813	the	811:813	CPP could improve plasma and liver lipid metabolism and accelerate the metabolism of the cecal total bile acids and short-chain fatty acids.
31855431	5	51	theme	fatty	854:858	arg1	acids					860:864	short-chain fatty acids	842:864	short-chain fatty acids	842:864	CPP could improve plasma and liver lipid metabolism and accelerate the metabolism of the cecal total bile acids and short-chain fatty acids.
31855431	0	52	theme	Green	58:62	arg1	pyrenoidosa					84:94	Green Microalga Chlorella pyrenoidosa	58:94	Green Microalga Chlorella pyrenoidosa	58:94	Physicochemical Characterization of a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats.
31855431	7	53	theme	flora	1183:1187	arg1	composition					1157:1167	the composition	1153:1167	the composition of intestinal flora	1153:1187	Moreover, with the 16S rRNA gene sequencing, it was revealed that the composition of intestinal flora changed drastically after treatment, such as the bloom of Coprococcus_1, Lactobacillus, and Turicibacter, whereas there was a strong reduction of the [Ruminococcus]_gauvreauii_group.
31855431	6	54	theme	carboxylase	980:990	arg1	expressions					1074:1084	the acetyl-CoA carboxylase, sterol regulatory element-binding protein 1c, and β-hydroxy β-methylglutaryl-CoA expressions	965:1084	the acetyl-CoA carboxylase, sterol regulatory element-binding protein 1c, and β-hydroxy β-methylglutaryl-CoA expressions	965:1084	CPP has also upregulated the adenosine-monophosphate-activated protein kinase α and downregulated the acetyl-CoA carboxylase, sterol regulatory element-binding protein 1c, and β-hydroxy β-methylglutaryl-CoA expressions.
31855431	7	55	theme	rRNA	1110:1113	arg1	sequencing					1120:1129	the 16S rRNA gene sequencing	1102:1129	the 16S rRNA gene sequencing	1102:1129	Moreover, with the 16S rRNA gene sequencing, it was revealed that the composition of intestinal flora changed drastically after treatment, such as the bloom of Coprococcus_1, Lactobacillus, and Turicibacter, whereas there was a strong reduction of the [Ruminococcus]_gauvreauii_group.
31855431	5	56	theme	acids	832:836	arg1	metabolism					797:806	the metabolism	793:806	the metabolism of the cecal total bile acids and short-chain fatty acids	793:864	CPP could improve plasma and liver lipid metabolism and accelerate the metabolism of the cecal total bile acids and short-chain fatty acids.
31855431	0	57	from	Activity	118:125	arg1	Rats					160:163	Rats	160:163	Rats	160:163	Physicochemical Characterization of a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats.
31855431	0	57	from	Activity	118:125	arg1	Characterization					16:31	Physicochemical Characterization	0:31	Physicochemical Characterization of a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats.	0:164	Physicochemical Characterization of a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats.
31855431	0	57	from	Activity	118:125	arg1	Polysaccharide					38:51	a Polysaccharide	36:51	a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats	36:163	Physicochemical Characterization of a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats.
31855431	2	58	theme	glycosidic	350:359	arg1	bonds					361:365	the main glycosidic bonds	341:365	the main glycosidic bonds	341:365	Nuclear magnetic resonance analysis showed that the main glycosidic bonds were composed of 1,2-linked-α-l-Fucp, 1,4-linked-α-l-Rhap, 1,4-linked-β-l-Araf, 1-linked-α-d-Glcp, 1,3-linked-β-d-GlcpA, 1,4-linked-β-d-Xylp, and 1,3,6-linked-β-d-Manp.
31855431	8	59	theme	lipid	1468:1472	arg1	metabolism					1474:1483	lipid metabolism disorders and intestinal flora in hyperlipidemia rats	1468:1537	metabolism	1474:1483	The above results illustrated that CPP might be served as an effective ingredient to ameliorate lipid metabolism disorders and intestinal flora in hyperlipidemia rats.
31855431	5	60	theme	short-chain	842:852	arg1	acids					860:864	short-chain fatty acids	842:864	short-chain fatty acids	842:864	CPP could improve plasma and liver lipid metabolism and accelerate the metabolism of the cecal total bile acids and short-chain fatty acids.
31855431	4	61	dep	histopathology	684:697	arg1	analyses					716:723	analyses	716:723	analyses	716:723	The hypolipidemic effect and intestinal flora regulation of CPP on diet-induced rats were evaluated through histopathology and biochemistry analyses.
31855431	1	62	from	pyrenoidosa	218:228	arg1	polysaccharide					178:191	A bioactive polysaccharide	166:191	A bioactive polysaccharide from microalga Chlorella pyrenoidosa (CPP)	166:234	A bioactive polysaccharide from microalga Chlorella pyrenoidosa (CPP) was successively prepared via DEAE-52 and G-100 columns.
31855431	7	63	theme	_gauvreauii_group	1353:1369	arg1	reduction					1322:1330	a strong reduction	1313:1330	a strong reduction of the [Ruminococcus]_gauvreauii_group	1313:1369	Moreover, with the 16S rRNA gene sequencing, it was revealed that the composition of intestinal flora changed drastically after treatment, such as the bloom of Coprococcus_1, Lactobacillus, and Turicibacter, whereas there was a strong reduction of the [Ruminococcus]_gauvreauii_group.
31855431	0	64	theme	Chlorella	74:82	arg1	pyrenoidosa					84:94	Green Microalga Chlorella pyrenoidosa	58:94	Green Microalga Chlorella pyrenoidosa	58:94	Physicochemical Characterization of a Polysaccharide from Green Microalga Chlorella pyrenoidosa and Its Hypolipidemic Activity via Gut Microbiota Regulation in Rats.
31855431	6	65	theme	β-methylglutaryl-CoA	1053:1072	arg1	expressions					1074:1084	the acetyl-CoA carboxylase, sterol regulatory element-binding protein 1c, and β-hydroxy β-methylglutaryl-CoA expressions	965:1084	the acetyl-CoA carboxylase, sterol regulatory element-binding protein 1c, and β-hydroxy β-methylglutaryl-CoA expressions	965:1084	CPP has also upregulated the adenosine-monophosphate-activated protein kinase α and downregulated the acetyl-CoA carboxylase, sterol regulatory element-binding protein 1c, and β-hydroxy β-methylglutaryl-CoA expressions.
31855431	7	66	theme	gene	1115:1118	arg1	sequencing					1120:1129	the 16S rRNA gene sequencing	1102:1129	the 16S rRNA gene sequencing	1102:1129	Moreover, with the 16S rRNA gene sequencing, it was revealed that the composition of intestinal flora changed drastically after treatment, such as the bloom of Coprococcus_1, Lactobacillus, and Turicibacter, whereas there was a strong reduction of the [Ruminococcus]_gauvreauii_group.
31855431	8	67	theme	effective	1433:1441	arg1	ingredient					1443:1452	an effective ingredient	1430:1452	an effective ingredient to ameliorate lipid metabolism disorders and intestinal flora in hyperlipidemia rats	1430:1537	The above results illustrated that CPP might be served as an effective ingredient to ameliorate lipid metabolism disorders and intestinal flora in hyperlipidemia rats.
31855431	5	68	theme	bile	827:830	arg1	acids					832:836	total bile acids	821:836	total bile acids	821:836	CPP could improve plasma and liver lipid metabolism and accelerate the metabolism of the cecal total bile acids and short-chain fatty acids.
32673722	0	0	theme	halotolerant	100:111	arg1	elongata					123:130	the extremely halotolerant Halomonas elongata	86:130	the extremely halotolerant Halomonas elongata	86:130	Structural characterization and functional properties of novel exopolysaccharide from the extremely halotolerant Halomonas elongata S6.
32673722	6	1	theme	several	1142:1148	arg1	fields					1150:1155	several fields	1142:1155	several fields such as in food, cosmetic and environmental industries	1142:1210	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	6	1	theme	several	1142:1148	arg1	industries					1201:1210	food, cosmetic and environmental industries	1168:1210	food, cosmetic and environmental industries	1168:1210	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	6	2	theme	pathogenic	938:947	arg1	biofilms					949:956	pathogenic biofilms	938:956	pathogenic biofilms	938:956	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	6	3	theme	DPPH•	861:865	arg1	inhibition					868:877	2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition	830:877	2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition	830:877	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	6	4	contain	has	789:791	arg2	activities					816:825	noticeable antioxidant activities	793:825	noticeable antioxidant activities	793:825	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	6	4	contain	has	789:791	arg1	It					786:787	It	786:787	It	786:787	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	6	5	from	concern	1131:1137	arg1	fields					1150:1155	several fields	1142:1155	several fields such as in food, cosmetic and environmental industries	1142:1210	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	6	5	from	concern	1131:1137	arg1	industries					1201:1210	food, cosmetic and environmental industries	1168:1210	food, cosmetic and environmental industries	1168:1210	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	0	6	theme	Halomonas	113:121	arg1	elongata					123:130	the extremely halotolerant Halomonas elongata	86:130	the extremely halotolerant Halomonas elongata	86:130	Structural characterization and functional properties of novel exopolysaccharide from the extremely halotolerant Halomonas elongata S6.
32673722	6	7	theme	2,2-diphenyl-1-picrylhydrazyl	830:858	arg1	inhibition					868:877	2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition	830:877	2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition	830:877	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	0	8	dep	S6	132:133	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and functional properties of novel exopolysaccharide from the extremely halotolerant Halomonas elongata S6.
32673722	0	8	dep	S6	132:133	arg1	properties					43:52	functional properties	32:52	functional properties	32:52	Structural characterization and functional properties of novel exopolysaccharide from the extremely halotolerant Halomonas elongata S6.
32673722	3	9	theme	HPAE-PAD	509:516	arg1	results					518:524	the HPAE-PAD results	505:524	the HPAE-PAD results	505:524	According to the HPAE-PAD results, EPS-S6 was mainly composed of glucosamine, mannose, rhamnose and glucose (1:0.9:0.7:0.3).
32673722	5	10	theme	several	758:764	arg1	features					776:783	several potential features	758:783	several potential features	758:783	Studies on its functional properties showed that EPS-S6 had several potential features.
32673722	4	11	theme	molecular	662:670	arg1	weight					672:677	its molecular weight	658:677	its molecular weight	658:677	EPS-S6 was highly negatively charged and its molecular weight was about 270 kDa.
32673722	6	12	theme	antioxidant	804:814	arg1	activities					816:825	noticeable antioxidant activities	793:825	noticeable antioxidant activities	793:825	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	2	13	theme	present	340:346	arg1	work					348:351	the present work	336:351	the present work	336:351	In the present work, a novel exopolysaccharide (EPS-S6) produced by the extremely halotolerant newly isolated Halomonas elongata strain S6, was characterized.
32673722	5	14	contain	had	754:756	arg2	features					776:783	several potential features	758:783	several potential features	758:783	Studies on its functional properties showed that EPS-S6 had several potential features.
32673722	5	14	contain	had	754:756	arg1	EPS-S6					747:752	EPS-S6	747:752	EPS-S6	747:752	Studies on its functional properties showed that EPS-S6 had several potential features.
32673722	6	15	theme	potential	1104:1112	arg1	concern					1131:1137	potential biotechnological concern	1104:1137	potential biotechnological concern in several fields such as in food, cosmetic and environmental industries	1104:1210	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	0	16	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and functional properties of novel exopolysaccharide from the extremely halotolerant Halomonas elongata S6.
32673722	6	17	theme	emulsifying	1019:1029	arg1	properties					1031:1040	interesting emulsifying properties	1007:1040	interesting emulsifying properties	1007:1040	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	6	18	theme	noticeable	793:802	arg1	activities					816:825	noticeable antioxidant activities	793:825	noticeable antioxidant activities	793:825	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	6	19	theme	environmental	1187:1199	arg1	industries					1201:1210	food, cosmetic and environmental industries	1168:1210	food, cosmetic and environmental industries	1168:1210	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	0	20	theme	functional	32:41	arg1	properties					43:52	functional properties	32:52	functional properties	32:52	Structural characterization and functional properties of novel exopolysaccharide from the extremely halotolerant Halomonas elongata S6.
32673722	1	21	theme	most	307:310	arg1	producers					322:330	the most potential producers	303:330	the most potential producers	303:330	Production of extracellular polysaccharides by halophilic Archaea and Bacteria has been widely reported and the members of the genus Halomonas have been identified as the most potential producers.
32673722	1	21	theme	most	307:310	arg1	members					248:254	the members	244:254	the members of the genus Halomonas	244:277	Production of extracellular polysaccharides by halophilic Archaea and Bacteria has been widely reported and the members of the genus Halomonas have been identified as the most potential producers.
32673722	2	22	theme	Halomonas	443:451	arg1	S6					469:470	the extremely halotolerant newly isolated Halomonas elongata strain S6	401:470	the extremely halotolerant newly isolated Halomonas elongata strain S6	401:470	In the present work, a novel exopolysaccharide (EPS-S6) produced by the extremely halotolerant newly isolated Halomonas elongata strain S6, was characterized.
32673722	2	23	theme	elongata	453:460	arg1	S6					469:470	the extremely halotolerant newly isolated Halomonas elongata strain S6	401:470	the extremely halotolerant newly isolated Halomonas elongata strain S6	401:470	In the present work, a novel exopolysaccharide (EPS-S6) produced by the extremely halotolerant newly isolated Halomonas elongata strain S6, was characterized.
32673722	1	24	theme	potential	312:320	arg1	producers					322:330	the most potential producers	303:330	the most potential producers	303:330	Production of extracellular polysaccharides by halophilic Archaea and Bacteria has been widely reported and the members of the genus Halomonas have been identified as the most potential producers.
32673722	1	24	theme	potential	312:320	arg1	members					248:254	the members	244:254	the members of the genus Halomonas	244:277	Production of extracellular polysaccharides by halophilic Archaea and Bacteria has been widely reported and the members of the genus Halomonas have been identified as the most potential producers.
32673722	5	25	theme	functional	713:722	arg1	properties					724:733	its functional properties	709:733	its functional properties	709:733	Studies on its functional properties showed that EPS-S6 had several potential features.
32673722	6	26	theme	cosmetic	1174:1181	arg1	industries					1201:1210	food, cosmetic and environmental industries	1168:1210	food, cosmetic and environmental industries	1168:1210	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	2	27	theme	isolated	434:441	arg1	S6					469:470	the extremely halotolerant newly isolated Halomonas elongata strain S6	401:470	the extremely halotolerant newly isolated Halomonas elongata strain S6	401:470	In the present work, a novel exopolysaccharide (EPS-S6) produced by the extremely halotolerant newly isolated Halomonas elongata strain S6, was characterized.
32673722	6	28	theme	suspension	992:1001	arg1	properties					1031:1040	interesting emulsifying properties	1007:1040	interesting emulsifying properties	1007:1040	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	6	28	theme	suspension	992:1001	arg1	biofilms					949:956	pathogenic biofilms	938:956	pathogenic biofilms	938:956	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	6	28	theme	suspension	992:1001	arg1	flocculation					969:980	excellent flocculation	959:980	excellent flocculation of kaolin suspension	959:1001	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	6	29	theme	good	899:902	arg1	ability					904:910	good ability	899:910	good ability	899:910	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	6	30	theme	kaolin	985:990	arg1	suspension					992:1001	kaolin suspension	985:1001	kaolin suspension	985:1001	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	2	31	theme	strain	462:467	arg1	S6					469:470	the extremely halotolerant newly isolated Halomonas elongata strain S6	401:470	the extremely halotolerant newly isolated Halomonas elongata strain S6	401:470	In the present work, a novel exopolysaccharide (EPS-S6) produced by the extremely halotolerant newly isolated Halomonas elongata strain S6, was characterized.
32673722	2	32	theme	halotolerant	415:426	arg1	S6					469:470	the extremely halotolerant newly isolated Halomonas elongata strain S6	401:470	the extremely halotolerant newly isolated Halomonas elongata strain S6	401:470	In the present work, a novel exopolysaccharide (EPS-S6) produced by the extremely halotolerant newly isolated Halomonas elongata strain S6, was characterized.
32673722	0	33	theme	exopolysaccharide	63:79	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and functional properties of novel exopolysaccharide from the extremely halotolerant Halomonas elongata S6.
32673722	0	33	theme	exopolysaccharide	63:79	arg1	properties					43:52	functional properties	32:52	functional properties	32:52	Structural characterization and functional properties of novel exopolysaccharide from the extremely halotolerant Halomonas elongata S6.
32673722	6	34	theme	interesting	1007:1017	arg1	properties					1031:1040	interesting emulsifying properties	1007:1040	interesting emulsifying properties	1007:1040	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	3	35	theme	glucose	592:598	arg1	glucosamine					557:567	glucosamine	557:567	glucosamine	557:567	According to the HPAE-PAD results, EPS-S6 was mainly composed of glucosamine, mannose, rhamnose and glucose (1:0.9:0.7:0.3).
32673722	2	36	theme	novel	356:360	arg1	EPS-S6					381:386	EPS-S6	381:386	EPS-S6	381:386	In the present work, a novel exopolysaccharide (EPS-S6) produced by the extremely halotolerant newly isolated Halomonas elongata strain S6, was characterized.
32673722	2	36	theme	novel	356:360	arg1	exopolysaccharide					362:378	a novel exopolysaccharide	354:378	a novel exopolysaccharide (EPS-S6)	354:387	In the present work, a novel exopolysaccharide (EPS-S6) produced by the extremely halotolerant newly isolated Halomonas elongata strain S6, was characterized.
32673722	0	37	theme	novel	57:61	arg1	exopolysaccharide					63:79	novel exopolysaccharide	57:79	novel exopolysaccharide	57:79	Structural characterization and functional properties of novel exopolysaccharide from the extremely halotolerant Halomonas elongata S6.
32673722	6	38	theme	DNA	883:885	arg1	protection					887:896	DNA protection	883:896	DNA protection	883:896	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	6	39	theme	food	1168:1171	arg1	industries					1201:1210	food, cosmetic and environmental industries	1168:1210	food, cosmetic and environmental industries	1168:1210	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	6	40	theme	excellent	959:967	arg1	flocculation					969:980	excellent flocculation	959:980	excellent flocculation of kaolin suspension	959:1001	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	1	41	theme	extracellular	150:162	arg1	polysaccharides					164:178	extracellular polysaccharides	150:178	extracellular polysaccharides	150:178	Production of extracellular polysaccharides by halophilic Archaea and Bacteria has been widely reported and the members of the genus Halomonas have been identified as the most potential producers.
32673722	5	42	from	Studies	698:704	arg1	properties					724:733	its functional properties	709:733	its functional properties	709:733	Studies on its functional properties showed that EPS-S6 had several potential features.
32673722	0	43	from	elongata	123:130	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and functional properties of novel exopolysaccharide from the extremely halotolerant Halomonas elongata S6.
32673722	0	43	from	elongata	123:130	arg1	properties					43:52	functional properties	32:52	functional properties	32:52	Structural characterization and functional properties of novel exopolysaccharide from the extremely halotolerant Halomonas elongata S6.
32673722	1	44	theme	polysaccharides	164:178	arg1	Production					136:145	Production	136:145	Production of extracellular polysaccharides by halophilic Archaea and Bacteria	136:213	Production of extracellular polysaccharides by halophilic Archaea and Bacteria has been widely reported and the members of the genus Halomonas have been identified as the most potential producers.
32673722	5	45	theme	potential	766:774	arg1	features					776:783	several potential features	758:783	several potential features	758:783	Studies on its functional properties showed that EPS-S6 had several potential features.
32673722	6	46	theme	biotechnological	1114:1129	arg1	concern					1131:1137	potential biotechnological concern	1104:1137	potential biotechnological concern in several fields such as in food, cosmetic and environmental industries	1104:1210	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	1	47	theme	genus	263:267	arg1	Halomonas					269:277	the genus Halomonas	259:277	the genus Halomonas	259:277	Production of extracellular polysaccharides by halophilic Archaea and Bacteria has been widely reported and the members of the genus Halomonas have been identified as the most potential producers.
32673722	6	48	contain	have	1099:1102	arg1	EPS-S6					1086:1091	EPS-S6	1086:1091	EPS-S6	1086:1091	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	6	48	contain	have	1099:1102	arg2	concern					1131:1137	potential biotechnological concern	1104:1137	potential biotechnological concern in several fields such as in food, cosmetic and environmental industries	1104:1210	It has noticeable antioxidant activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH•) inhibition and DNA protection, good ability to inhibit and to disrupt pathogenic biofilms, excellent flocculation of kaolin suspension and interesting emulsifying properties at acidic, neutral and basic pH. Therefore, EPS-S6 could have potential biotechnological concern in several fields such as in food, cosmetic and environmental industries.
32673722	1	49	theme	halophilic	183:192	arg1	Archaea					194:200	halophilic Archaea	183:200	halophilic Archaea	183:200	Production of extracellular polysaccharides by halophilic Archaea and Bacteria has been widely reported and the members of the genus Halomonas have been identified as the most potential producers.
32673722	1	50	theme	Halomonas	269:277	arg1	producers					322:330	the most potential producers	303:330	the most potential producers	303:330	Production of extracellular polysaccharides by halophilic Archaea and Bacteria has been widely reported and the members of the genus Halomonas have been identified as the most potential producers.
32673722	1	50	theme	Halomonas	269:277	arg1	members					248:254	the members	244:254	the members of the genus Halomonas	244:277	Production of extracellular polysaccharides by halophilic Archaea and Bacteria has been widely reported and the members of the genus Halomonas have been identified as the most potential producers.
33098894	2	0	theme	MCC/TPS	354:360	arg1	films					372:376	the MCC/TPS composite films	350:376	the MCC/TPS composite films	350:376	Then the MCC/TPS composite films were produced by the hot-pressing method, which were characterized by XRD, FTIR, SEM, DSC, TGA, tensile test, water vapor permeation and water contact angle.
33098894	1	1	theme	prepared	239:246	arg1	granules					279:286	prepared MCC/thermoplastic starch (TPS) granules	239:286	prepared MCC/thermoplastic starch (TPS) granules	239:286	The microcrystalline cellulose (MCC) used as the fillers, were incorporated into the starch matrix to prepared MCC/thermoplastic starch (TPS) granules by extrusion method blending with glycerol plasticizers.
33098894	0	2	theme	packaging	87:95	arg1	composites					97:106	packaging composites	87:106	packaging composites	87:106	Preparation and performance of thermoplastic starch and microcrystalline cellulose for packaging composites: Extrusion and hot pressing.
33098894	6	3	theme	contact	973:979	arg1	angle					981:985	the water contact angle	963:985	the water contact angle of MCC/TPS composite films	963:1012	Besides, the water contact angle of MCC/TPS composite films decreased gradually with the content of MCC increased.
33098894	1	4	theme	MCC/thermoplastic	248:264	arg1	granules					279:286	prepared MCC/thermoplastic starch (TPS) granules	239:286	prepared MCC/thermoplastic starch (TPS) granules	239:286	The microcrystalline cellulose (MCC) used as the fillers, were incorporated into the starch matrix to prepared MCC/thermoplastic starch (TPS) granules by extrusion method blending with glycerol plasticizers.
33098894	6	5	theme	water	967:971	arg1	angle					981:985	the water contact angle	963:985	the water contact angle of MCC/TPS composite films	963:1012	Besides, the water contact angle of MCC/TPS composite films decreased gradually with the content of MCC increased.
33098894	3	6	theme	composite	612:620	arg1	films					622:626	composite films	612:626	composite films seen from SEM images	612:647	When the loading of MCC was not more than 6 wt%, MCC were well dispersed in composite films seen from SEM images.
33098894	1	7	theme	starch	266:271	arg1	granules					279:286	prepared MCC/thermoplastic starch (TPS) granules	239:286	prepared MCC/thermoplastic starch (TPS) granules	239:286	The microcrystalline cellulose (MCC) used as the fillers, were incorporated into the starch matrix to prepared MCC/thermoplastic starch (TPS) granules by extrusion method blending with glycerol plasticizers.
33098894	6	8	theme	MCC	1054:1056	arg1	content					1043:1049	the content	1039:1049	the content of MCC	1039:1056	Besides, the water contact angle of MCC/TPS composite films decreased gradually with the content of MCC increased.
33098894	4	9	from	crystallinity	676:688	arg1	presence					704:711	the presence	700:711	the presence of MCC	700:718	XRD results show that the crystallinity of TPS in the presence of MCC was improved.
33098894	5	10	theme	water	859:863	arg1	permeation					871:880	water vapor permeation	859:880	water vapor permeation	859:880	The FTIR curves indicate that the interaction between MCC and starch formed more hydrogen bonds, so the tensile strength and water vapor permeation of 6 wt% MCC/TPS composite films were better than those of other films.
33098894	2	11	theme	tensile	474:480	arg1	test					482:485	tensile test	474:485	tensile test	474:485	Then the MCC/TPS composite films were produced by the hot-pressing method, which were characterized by XRD, FTIR, SEM, DSC, TGA, tensile test, water vapor permeation and water contact angle.
33098894	6	12	theme	films	1008:1012	arg1	angle					981:985	the water contact angle	963:985	the water contact angle of MCC/TPS composite films	963:1012	Besides, the water contact angle of MCC/TPS composite films decreased gradually with the content of MCC increased.
33098894	4	13	theme	TPS	693:695	arg1	crystallinity					676:688	the crystallinity	672:688	the crystallinity of TPS in the presence of MCC	672:718	XRD results show that the crystallinity of TPS in the presence of MCC was improved.
33098894	0	14	theme	hot	123:125	arg1	pressing					127:134	hot pressing	123:134	hot pressing	123:134	Preparation and performance of thermoplastic starch and microcrystalline cellulose for packaging composites: Extrusion and hot pressing.
33098894	3	15	theme	SEM	638:640	arg1	images					642:647	SEM images	638:647	SEM images	638:647	When the loading of MCC was not more than 6 wt%, MCC were well dispersed in composite films seen from SEM images.
33098894	1	16	theme	TPS	274:276	arg1	granules					279:286	prepared MCC/thermoplastic starch (TPS) granules	239:286	prepared MCC/thermoplastic starch (TPS) granules	239:286	The microcrystalline cellulose (MCC) used as the fillers, were incorporated into the starch matrix to prepared MCC/thermoplastic starch (TPS) granules by extrusion method blending with glycerol plasticizers.
33098894	4	17	theme	XRD	650:652	arg1	results					654:660	XRD results	650:660	XRD results	650:660	XRD results show that the crystallinity of TPS in the presence of MCC was improved.
33098894	5	18	dep	strength	846:853	arg1	the					834:836	the	834:836	the	834:836	The FTIR curves indicate that the interaction between MCC and starch formed more hydrogen bonds, so the tensile strength and water vapor permeation of 6 wt% MCC/TPS composite films were better than those of other films.
33098894	5	19	theme	%	889:889	arg1	films					909:913	6 wt% MCC/TPS composite films	885:913	6 wt% MCC/TPS composite films	885:913	The FTIR curves indicate that the interaction between MCC and starch formed more hydrogen bonds, so the tensile strength and water vapor permeation of 6 wt% MCC/TPS composite films were better than those of other films.
33098894	5	20	theme	tensile	838:844	arg1	strength					846:853	tensile strength	838:853	tensile strength	838:853	The FTIR curves indicate that the interaction between MCC and starch formed more hydrogen bonds, so the tensile strength and water vapor permeation of 6 wt% MCC/TPS composite films were better than those of other films.
33098894	5	21	theme	vapor	865:869	arg1	permeation					871:880	water vapor permeation	859:880	water vapor permeation	859:880	The FTIR curves indicate that the interaction between MCC and starch formed more hydrogen bonds, so the tensile strength and water vapor permeation of 6 wt% MCC/TPS composite films were better than those of other films.
33098894	5	22	theme	FTIR	738:741	arg1	curves					743:748	The FTIR curves	734:748	The FTIR curves	734:748	The FTIR curves indicate that the interaction between MCC and starch formed more hydrogen bonds, so the tensile strength and water vapor permeation of 6 wt% MCC/TPS composite films were better than those of other films.
33098894	0	23	theme	starch	45:50	arg1	performance					16:26	performance	16:26	performance	16:26	Preparation and performance of thermoplastic starch and microcrystalline cellulose for packaging composites: Extrusion and hot pressing.
33098894	0	23	theme	starch	45:50	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and performance of thermoplastic starch and microcrystalline cellulose for packaging composites: Extrusion and hot pressing.
33098894	6	24	theme	composite	998:1006	arg1	films					1008:1012	MCC/TPS composite films	990:1012	MCC/TPS composite films	990:1012	Besides, the water contact angle of MCC/TPS composite films decreased gradually with the content of MCC increased.
33098894	2	25	theme	vapor	494:498	arg1	permeation					500:509	water vapor permeation	488:509	water vapor permeation	488:509	Then the MCC/TPS composite films were produced by the hot-pressing method, which were characterized by XRD, FTIR, SEM, DSC, TGA, tensile test, water vapor permeation and water contact angle.
33098894	5	26	theme	composite	899:907	arg1	films					909:913	6 wt% MCC/TPS composite films	885:913	6 wt% MCC/TPS composite films	885:913	The FTIR curves indicate that the interaction between MCC and starch formed more hydrogen bonds, so the tensile strength and water vapor permeation of 6 wt% MCC/TPS composite films were better than those of other films.
33098894	0	27	theme	thermoplastic	31:43	arg1	starch					45:50	thermoplastic starch	31:50	thermoplastic starch	31:50	Preparation and performance of thermoplastic starch and microcrystalline cellulose for packaging composites: Extrusion and hot pressing.
33098894	1	28	theme	extrusion	291:299	arg1	method					301:306	extrusion method	291:306	extrusion method blending with glycerol plasticizers	291:342	The microcrystalline cellulose (MCC) used as the fillers, were incorporated into the starch matrix to prepared MCC/thermoplastic starch (TPS) granules by extrusion method blending with glycerol plasticizers.
33098894	2	29	theme	water	488:492	arg1	permeation					500:509	water vapor permeation	488:509	water vapor permeation	488:509	Then the MCC/TPS composite films were produced by the hot-pressing method, which were characterized by XRD, FTIR, SEM, DSC, TGA, tensile test, water vapor permeation and water contact angle.
33098894	4	30	theme	MCC	716:718	arg1	presence					704:711	the presence	700:711	the presence of MCC	700:718	XRD results show that the crystallinity of TPS in the presence of MCC was improved.
33098894	2	31	theme	hot-pressing	399:410	arg1	method					412:417	the hot-pressing method	395:417	the hot-pressing method	395:417	Then the MCC/TPS composite films were produced by the hot-pressing method, which were characterized by XRD, FTIR, SEM, DSC, TGA, tensile test, water vapor permeation and water contact angle.
33098894	0	32	theme	microcrystalline	56:71	arg1	cellulose					73:81	microcrystalline cellulose	56:81	microcrystalline cellulose	56:81	Preparation and performance of thermoplastic starch and microcrystalline cellulose for packaging composites: Extrusion and hot pressing.
33098894	5	33	theme	more	810:813	arg1	bonds					824:828	more hydrogen bonds	810:828	more hydrogen bonds	810:828	The FTIR curves indicate that the interaction between MCC and starch formed more hydrogen bonds, so the tensile strength and water vapor permeation of 6 wt% MCC/TPS composite films were better than those of other films.
33098894	5	34	theme	hydrogen	815:822	arg1	bonds					824:828	more hydrogen bonds	810:828	more hydrogen bonds	810:828	The FTIR curves indicate that the interaction between MCC and starch formed more hydrogen bonds, so the tensile strength and water vapor permeation of 6 wt% MCC/TPS composite films were better than those of other films.
33098894	5	35	theme	MCC/TPS	891:897	arg1	films					909:913	6 wt% MCC/TPS composite films	885:913	6 wt% MCC/TPS composite films	885:913	The FTIR curves indicate that the interaction between MCC and starch formed more hydrogen bonds, so the tensile strength and water vapor permeation of 6 wt% MCC/TPS composite films were better than those of other films.
33098894	2	36	theme	contact	521:527	arg1	angle					529:533	water contact angle	515:533	water contact angle	515:533	Then the MCC/TPS composite films were produced by the hot-pressing method, which were characterized by XRD, FTIR, SEM, DSC, TGA, tensile test, water vapor permeation and water contact angle.
33098894	5	37	theme	films	909:913	arg1	strength					846:853	tensile strength	838:853	tensile strength	838:853	The FTIR curves indicate that the interaction between MCC and starch formed more hydrogen bonds, so the tensile strength and water vapor permeation of 6 wt% MCC/TPS composite films were better than those of other films.
33098894	5	37	theme	films	909:913	arg1	permeation					871:880	water vapor permeation	859:880	water vapor permeation	859:880	The FTIR curves indicate that the interaction between MCC and starch formed more hydrogen bonds, so the tensile strength and water vapor permeation of 6 wt% MCC/TPS composite films were better than those of other films.
33098894	6	38	theme	MCC/TPS	990:996	arg1	films					1008:1012	MCC/TPS composite films	990:1012	MCC/TPS composite films	990:1012	Besides, the water contact angle of MCC/TPS composite films decreased gradually with the content of MCC increased.
33098894	2	39	theme	water	515:519	arg1	angle					529:533	water contact angle	515:533	water contact angle	515:533	Then the MCC/TPS composite films were produced by the hot-pressing method, which were characterized by XRD, FTIR, SEM, DSC, TGA, tensile test, water vapor permeation and water contact angle.
33098894	5	40	theme	6 wt	885:888	arg1	films					909:913	6 wt% MCC/TPS composite films	885:913	6 wt% MCC/TPS composite films	885:913	The FTIR curves indicate that the interaction between MCC and starch formed more hydrogen bonds, so the tensile strength and water vapor permeation of 6 wt% MCC/TPS composite films were better than those of other films.
33098894	1	41	theme	microcrystalline	141:156	arg1	MCC					169:171	MCC	169:171	MCC	169:171	The microcrystalline cellulose (MCC) used as the fillers, were incorporated into the starch matrix to prepared MCC/thermoplastic starch (TPS) granules by extrusion method blending with glycerol plasticizers.
33098894	1	41	theme	microcrystalline	141:156	arg1	cellulose					158:166	The microcrystalline cellulose	137:166	The microcrystalline cellulose (MCC)	137:172	The microcrystalline cellulose (MCC) used as the fillers, were incorporated into the starch matrix to prepared MCC/thermoplastic starch (TPS) granules by extrusion method blending with glycerol plasticizers.
33098894	1	42	theme	starch	222:227	arg1	matrix					229:234	the starch matrix	218:234	the starch matrix to prepared MCC/thermoplastic starch (TPS) granules	218:286	The microcrystalline cellulose (MCC) used as the fillers, were incorporated into the starch matrix to prepared MCC/thermoplastic starch (TPS) granules by extrusion method blending with glycerol plasticizers.
33098894	5	43	theme	other	941:945	arg1	films					947:951	other films	941:951	other films	941:951	The FTIR curves indicate that the interaction between MCC and starch formed more hydrogen bonds, so the tensile strength and water vapor permeation of 6 wt% MCC/TPS composite films were better than those of other films.
33098894	3	44	theme	MCC	556:558	arg1	loading					545:551	the loading	541:551	the loading of MCC	541:558	When the loading of MCC was not more than 6 wt%, MCC were well dispersed in composite films seen from SEM images.
33098894	3	44	theme	MCC	556:558	arg1	more					568:571	more	568:571	more	568:571	When the loading of MCC was not more than 6 wt%, MCC were well dispersed in composite films seen from SEM images.
33098894	0	45	theme	cellulose	73:81	arg1	performance					16:26	performance	16:26	performance	16:26	Preparation and performance of thermoplastic starch and microcrystalline cellulose for packaging composites: Extrusion and hot pressing.
33098894	0	45	theme	cellulose	73:81	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and performance of thermoplastic starch and microcrystalline cellulose for packaging composites: Extrusion and hot pressing.
33098894	1	46	theme	glycerol	322:329	arg1	plasticizers					331:342	glycerol plasticizers	322:342	glycerol plasticizers	322:342	The microcrystalline cellulose (MCC) used as the fillers, were incorporated into the starch matrix to prepared MCC/thermoplastic starch (TPS) granules by extrusion method blending with glycerol plasticizers.
33098894	0	47	dep	Preparation	0:10	arg1	Extrusion					109:117	Extrusion	109:117	Extrusion	109:117	Preparation and performance of thermoplastic starch and microcrystalline cellulose for packaging composites: Extrusion and hot pressing.
33098894	0	47	dep	Preparation	0:10	arg1	pressing					127:134	hot pressing	123:134	hot pressing	123:134	Preparation and performance of thermoplastic starch and microcrystalline cellulose for packaging composites: Extrusion and hot pressing.
33098894	2	48	theme	composite	362:370	arg1	films					372:376	the MCC/TPS composite films	350:376	the MCC/TPS composite films	350:376	Then the MCC/TPS composite films were produced by the hot-pressing method, which were characterized by XRD, FTIR, SEM, DSC, TGA, tensile test, water vapor permeation and water contact angle.
34509152	4	0	from	sources	594:600	arg1	molecules					571:579	bioactive molecules	561:579	bioactive molecules from natural sources such as plants and fungi	561:625	In natural products chemistry, dereplication techniques identify bioactive molecules from natural sources such as plants and fungi without isolating or characterizing molecules.
34509152	4	0	from	sources	594:600	arg1	fungi					621:625	fungi	621:625	fungi	621:625	In natural products chemistry, dereplication techniques identify bioactive molecules from natural sources such as plants and fungi without isolating or characterizing molecules.
34509152	4	0	from	sources	594:600	arg1	plants					610:615	plants	610:615	plants	610:615	In natural products chemistry, dereplication techniques identify bioactive molecules from natural sources such as plants and fungi without isolating or characterizing molecules.
34509152	1	1	theme	Basidiomycetes	160:173	arg1	genus					151:155	the well-known genus	136:155	the well-known genus of Basidiomycetes	136:173	Pleurotus is an edible mushroom from the well-known genus of Basidiomycetes; it is the second-most commonly consumed mushroom worldwide.
34509152	4	2	theme	bioactive	561:569	arg1	molecules					571:579	bioactive molecules	561:579	bioactive molecules from natural sources such as plants and fungi	561:625	In natural products chemistry, dereplication techniques identify bioactive molecules from natural sources such as plants and fungi without isolating or characterizing molecules.
34509152	4	2	theme	bioactive	561:569	arg1	fungi					621:625	fungi	621:625	fungi	621:625	In natural products chemistry, dereplication techniques identify bioactive molecules from natural sources such as plants and fungi without isolating or characterizing molecules.
34509152	4	2	theme	bioactive	561:569	arg1	plants					610:615	plants	610:615	plants	610:615	In natural products chemistry, dereplication techniques identify bioactive molecules from natural sources such as plants and fungi without isolating or characterizing molecules.
34509152	7	3	theme	fatty	1104:1108	arg1	acids					1110:1114	eight fatty acids	1098:1114	eight fatty acids	1098:1114	Using this technique, we identified several molecular families, including eight fatty acids and seven steroids.
34509152	5	4	theme	Global	720:725	arg1	Products					735:742	the Global Natural Products	716:742	the Global Natural Products	716:742	We used dereplication techniques aided by the Global Natural Products Social Molecular Network to analyze the chemical composition of the methanolic extracts of six Pleurotus species (P. sapidus, P. ostreaus, P. ostreaus var.
34509152	2	5	theme	fatty	293:297	arg1	acids					299:303	fatty acids	293:303	fatty acids	293:303	This genus is characterized by the presence of steroids, fatty acids, and polysaccharides.
34509152	2	6	theme	acids	299:303	arg1	presence					271:278	the presence	267:278	the presence of steroids, fatty acids, and polysaccharides	267:324	This genus is characterized by the presence of steroids, fatty acids, and polysaccharides.
34509152	5	7	theme	Natural	727:733	arg1	Products					735:742	the Global Natural Products	716:742	the Global Natural Products	716:742	We used dereplication techniques aided by the Global Natural Products Social Molecular Network to analyze the chemical composition of the methanolic extracts of six Pleurotus species (P. sapidus, P. ostreaus, P. ostreaus var.
34509152	1	8	from	genus	151:155	arg1	mushroom					122:129	an edible mushroom	112:129	an edible mushroom from the well-known genus of Basidiomycetes	112:173	Pleurotus is an edible mushroom from the well-known genus of Basidiomycetes; it is the second-most commonly consumed mushroom worldwide.
34509152	1	8	from	genus	151:155	arg1	Pleurotus					99:107	Pleurotus	99:107	Pleurotus	99:107	Pleurotus is an edible mushroom from the well-known genus of Basidiomycetes; it is the second-most commonly consumed mushroom worldwide.
34509152	3	9	theme	minerals	474:481	arg1	they					435:438	they	435:438	they	435:438	Recently, Pleurotus has become popular as a functional food owing to its health benefits, primarily because they are a source of vitamins, fibers, minerals, and lipids.
34509152	3	9	theme	minerals	474:481	arg1	source					446:451	a source	444:451	a source of vitamins, fibers, minerals, and lipids	444:493	Recently, Pleurotus has become popular as a functional food owing to its health benefits, primarily because they are a source of vitamins, fibers, minerals, and lipids.
34509152	7	10	theme	molecular	1068:1076	arg1	steroids					1126:1133	seven steroids	1120:1133	seven steroids	1120:1133	Using this technique, we identified several molecular families, including eight fatty acids and seven steroids.
34509152	7	10	theme	molecular	1068:1076	arg1	acids					1110:1114	eight fatty acids	1098:1114	eight fatty acids	1098:1114	Using this technique, we identified several molecular families, including eight fatty acids and seven steroids.
34509152	7	10	theme	molecular	1068:1076	arg1	families					1078:1085	several molecular families	1060:1085	several molecular families	1060:1085	Using this technique, we identified several molecular families, including eight fatty acids and seven steroids.
34509152	5	11	theme	Pleurotus	839:847	arg1	species					849:855	six Pleurotus species	835:855	six Pleurotus species	835:855	We used dereplication techniques aided by the Global Natural Products Social Molecular Network to analyze the chemical composition of the methanolic extracts of six Pleurotus species (P. sapidus, P. ostreaus, P. ostreaus var.
34509152	2	12	theme	steroids	283:290	arg1	presence					271:278	the presence	267:278	the presence of steroids, fatty acids, and polysaccharides	267:324	This genus is characterized by the presence of steroids, fatty acids, and polysaccharides.
34509152	8	13	theme	fungal	1222:1227	arg1	species					1229:1235	fungal species	1222:1235	fungal species	1222:1235	Our findings suggest that dereplication is a relatively rapid tool for characterizing fungal species and determining their nutraceutical value.
34509152	5	14	theme	Social	744:749	arg1	Network					761:767	Social Molecular Network	744:767	Social Molecular Network	744:767	We used dereplication techniques aided by the Global Natural Products Social Molecular Network to analyze the chemical composition of the methanolic extracts of six Pleurotus species (P. sapidus, P. ostreaus, P. ostreaus var.
34509152	0	15	from	mushrooms	45:53	arg1	extracts					17:24	extracts	17:24	extracts from nutraceutical mushrooms	17:53	Dereplication of extracts from nutraceutical mushrooms Pleurotus using molecular network approach.
34509152	8	16	theme	rapid	1192:1196	arg1	tool					1198:1201	a relatively rapid tool	1179:1201	a relatively rapid tool for characterizing fungal species and determining their nutraceutical value	1179:1277	Our findings suggest that dereplication is a relatively rapid tool for characterizing fungal species and determining their nutraceutical value.
34509152	8	16	theme	rapid	1192:1196	arg1	dereplication					1162:1174	dereplication	1162:1174	dereplication	1162:1174	Our findings suggest that dereplication is a relatively rapid tool for characterizing fungal species and determining their nutraceutical value.
34509152	5	17	theme	methanolic	812:821	arg1	extracts					823:830	the methanolic extracts	808:830	the methanolic extracts of six Pleurotus species	808:855	We used dereplication techniques aided by the Global Natural Products Social Molecular Network to analyze the chemical composition of the methanolic extracts of six Pleurotus species (P. sapidus, P. ostreaus, P. ostreaus var.
34509152	5	18	theme	Molecular	751:759	arg1	Network					761:767	Social Molecular Network	744:767	Social Molecular Network	744:767	We used dereplication techniques aided by the Global Natural Products Social Molecular Network to analyze the chemical composition of the methanolic extracts of six Pleurotus species (P. sapidus, P. ostreaus, P. ostreaus var.
34509152	2	19	theme	polysaccharides	310:324	arg1	presence					271:278	the presence	267:278	the presence of steroids, fatty acids, and polysaccharides	267:324	This genus is characterized by the presence of steroids, fatty acids, and polysaccharides.
34509152	5	20	used	used	677:680	arg2	We					674:675	We	674:675	We	674:675	We used dereplication techniques aided by the Global Natural Products Social Molecular Network to analyze the chemical composition of the methanolic extracts of six Pleurotus species (P. sapidus, P. ostreaus, P. ostreaus var.
34509152	7	21	theme	several	1060:1066	arg1	steroids					1126:1133	seven steroids	1120:1133	seven steroids	1120:1133	Using this technique, we identified several molecular families, including eight fatty acids and seven steroids.
34509152	7	21	theme	several	1060:1066	arg1	acids					1110:1114	eight fatty acids	1098:1114	eight fatty acids	1098:1114	Using this technique, we identified several molecular families, including eight fatty acids and seven steroids.
34509152	7	21	theme	several	1060:1066	arg1	families					1078:1085	several molecular families	1060:1085	several molecular families	1060:1085	Using this technique, we identified several molecular families, including eight fatty acids and seven steroids.
34509152	1	22	theme	second-most	186:196	arg1	it					176:177	it	176:177	it	176:177	Pleurotus is an edible mushroom from the well-known genus of Basidiomycetes; it is the second-most commonly consumed mushroom worldwide.
34509152	1	22	theme	second-most	186:196	arg1	worldwide					225:233	the second-most commonly consumed mushroom worldwide	182:233	the second-most commonly consumed mushroom worldwide	182:233	Pleurotus is an edible mushroom from the well-known genus of Basidiomycetes; it is the second-most commonly consumed mushroom worldwide.
34509152	5	23	theme	extracts	823:830	arg1	composition					793:803	the chemical composition	780:803	the chemical composition of the methanolic extracts of six Pleurotus species	780:855	We used dereplication techniques aided by the Global Natural Products Social Molecular Network to analyze the chemical composition of the methanolic extracts of six Pleurotus species (P. sapidus, P. ostreaus, P. ostreaus var.
34509152	3	24	theme	functional	371:380	arg1	food					382:385	a functional food	369:385	a functional food owing to its health benefits	369:414	Recently, Pleurotus has become popular as a functional food owing to its health benefits, primarily because they are a source of vitamins, fibers, minerals, and lipids.
34509152	5	25	dep	analyze	772:778	arg1	ostreaus					873:880	ostreaus	873:880	ostreaus	873:880	We used dereplication techniques aided by the Global Natural Products Social Molecular Network to analyze the chemical composition of the methanolic extracts of six Pleurotus species (P. sapidus, P. ostreaus, P. ostreaus var.
34509152	5	25	dep	analyze	772:778	arg1	var					895:897	var	895:897	var	895:897	We used dereplication techniques aided by the Global Natural Products Social Molecular Network to analyze the chemical composition of the methanolic extracts of six Pleurotus species (P. sapidus, P. ostreaus, P. ostreaus var.
34509152	5	25	dep	analyze	772:778	arg1	P.					858:859	P.	858:859	P.	858:859	We used dereplication techniques aided by the Global Natural Products Social Molecular Network to analyze the chemical composition of the methanolic extracts of six Pleurotus species (P. sapidus, P. ostreaus, P. ostreaus var.
34509152	1	26	theme	edible	115:120	arg1	mushroom					122:129	an edible mushroom	112:129	an edible mushroom from the well-known genus of Basidiomycetes	112:173	Pleurotus is an edible mushroom from the well-known genus of Basidiomycetes; it is the second-most commonly consumed mushroom worldwide.
34509152	1	26	theme	edible	115:120	arg1	Pleurotus					99:107	Pleurotus	99:107	Pleurotus	99:107	Pleurotus is an edible mushroom from the well-known genus of Basidiomycetes; it is the second-most commonly consumed mushroom worldwide.
34509152	1	27	theme	consumed	207:214	arg1	it					176:177	it	176:177	it	176:177	Pleurotus is an edible mushroom from the well-known genus of Basidiomycetes; it is the second-most commonly consumed mushroom worldwide.
34509152	1	27	theme	consumed	207:214	arg1	worldwide					225:233	the second-most commonly consumed mushroom worldwide	182:233	the second-most commonly consumed mushroom worldwide	182:233	Pleurotus is an edible mushroom from the well-known genus of Basidiomycetes; it is the second-most commonly consumed mushroom worldwide.
34509152	3	28	theme	health	400:405	arg1	benefits					407:414	its health benefits	396:414	its health benefits	396:414	Recently, Pleurotus has become popular as a functional food owing to its health benefits, primarily because they are a source of vitamins, fibers, minerals, and lipids.
34509152	4	29	theme	dereplication	527:539	arg1	techniques					541:550	dereplication techniques	527:550	dereplication techniques	527:550	In natural products chemistry, dereplication techniques identify bioactive molecules from natural sources such as plants and fungi without isolating or characterizing molecules.
34509152	5	30	theme	species	849:855	arg1	extracts					823:830	the methanolic extracts	808:830	the methanolic extracts of six Pleurotus species	808:855	We used dereplication techniques aided by the Global Natural Products Social Molecular Network to analyze the chemical composition of the methanolic extracts of six Pleurotus species (P. sapidus, P. ostreaus, P. ostreaus var.
34509152	1	31	theme	mushroom	216:223	arg1	it					176:177	it	176:177	it	176:177	Pleurotus is an edible mushroom from the well-known genus of Basidiomycetes; it is the second-most commonly consumed mushroom worldwide.
34509152	1	31	theme	mushroom	216:223	arg1	worldwide					225:233	the second-most commonly consumed mushroom worldwide	182:233	the second-most commonly consumed mushroom worldwide	182:233	Pleurotus is an edible mushroom from the well-known genus of Basidiomycetes; it is the second-most commonly consumed mushroom worldwide.
34509152	0	32	theme	nutraceutical	31:43	arg1	mushrooms					45:53	nutraceutical mushrooms	31:53	nutraceutical mushrooms	31:53	Dereplication of extracts from nutraceutical mushrooms Pleurotus using molecular network approach.
34509152	5	33	theme	chemical	784:791	arg1	composition					793:803	the chemical composition	780:803	the chemical composition of the methanolic extracts of six Pleurotus species	780:855	We used dereplication techniques aided by the Global Natural Products Social Molecular Network to analyze the chemical composition of the methanolic extracts of six Pleurotus species (P. sapidus, P. ostreaus, P. ostreaus var.
34509152	6	34	theme	nutraceutical	998:1010	arg1	properties					1012:1021	nutraceutical properties	998:1021	nutraceutical properties	998:1021	Florida, P. djamor, P. citrinopileatus, and P. pulmonarius), to identify bioactive molecules with nutraceutical properties.
34509152	5	35	dep	P.	858:859	arg1	sapidus					861:867	sapidus	861:867	P. sapidus	858:867	We used dereplication techniques aided by the Global Natural Products Social Molecular Network to analyze the chemical composition of the methanolic extracts of six Pleurotus species (P. sapidus, P. ostreaus, P. ostreaus var.
34509152	3	36	theme	fibers	466:471	arg1	they					435:438	they	435:438	they	435:438	Recently, Pleurotus has become popular as a functional food owing to its health benefits, primarily because they are a source of vitamins, fibers, minerals, and lipids.
34509152	3	36	theme	fibers	466:471	arg1	source					446:451	a source	444:451	a source of vitamins, fibers, minerals, and lipids	444:493	Recently, Pleurotus has become popular as a functional food owing to its health benefits, primarily because they are a source of vitamins, fibers, minerals, and lipids.
34509152	4	37	theme	products	507:514	arg1	chemistry					516:524	natural products chemistry	499:524	natural products chemistry	499:524	In natural products chemistry, dereplication techniques identify bioactive molecules from natural sources such as plants and fungi without isolating or characterizing molecules.
34509152	6	38	with	molecules	983:991	arg1	properties					1012:1021	nutraceutical properties	998:1021	nutraceutical properties	998:1021	Florida, P. djamor, P. citrinopileatus, and P. pulmonarius), to identify bioactive molecules with nutraceutical properties.
34509152	4	39	theme	natural	499:505	arg1	products					507:514	natural products	499:514	natural products chemistry	499:524	In natural products chemistry, dereplication techniques identify bioactive molecules from natural sources such as plants and fungi without isolating or characterizing molecules.
34509152	3	40	theme	vitamins	456:463	arg1	they					435:438	they	435:438	they	435:438	Recently, Pleurotus has become popular as a functional food owing to its health benefits, primarily because they are a source of vitamins, fibers, minerals, and lipids.
34509152	3	40	theme	vitamins	456:463	arg1	source					446:451	a source	444:451	a source of vitamins, fibers, minerals, and lipids	444:493	Recently, Pleurotus has become popular as a functional food owing to its health benefits, primarily because they are a source of vitamins, fibers, minerals, and lipids.
34509152	5	41	theme	dereplication	682:694	arg1	techniques					696:705	dereplication techniques	682:705	dereplication techniques aided by the Global Natural Products	682:742	We used dereplication techniques aided by the Global Natural Products Social Molecular Network to analyze the chemical composition of the methanolic extracts of six Pleurotus species (P. sapidus, P. ostreaus, P. ostreaus var.
34509152	1	42	theme	well-known	140:149	arg1	genus					151:155	the well-known genus	136:155	the well-known genus of Basidiomycetes	136:173	Pleurotus is an edible mushroom from the well-known genus of Basidiomycetes; it is the second-most commonly consumed mushroom worldwide.
34509152	4	43	theme	natural	586:592	arg1	sources					594:600	natural sources	586:600	natural sources	586:600	In natural products chemistry, dereplication techniques identify bioactive molecules from natural sources such as plants and fungi without isolating or characterizing molecules.
34509152	0	44	theme	network	81:87	arg1	approach					89:96	molecular network approach	71:96	molecular network approach	71:96	Dereplication of extracts from nutraceutical mushrooms Pleurotus using molecular network approach.
34509152	8	45	theme	nutraceutical	1259:1271	arg1	value					1273:1277	their nutraceutical value	1253:1277	their nutraceutical value	1253:1277	Our findings suggest that dereplication is a relatively rapid tool for characterizing fungal species and determining their nutraceutical value.
34509152	6	46	theme	bioactive	973:981	arg1	molecules					983:991	bioactive molecules	973:991	bioactive molecules with nutraceutical properties	973:1021	Florida, P. djamor, P. citrinopileatus, and P. pulmonarius), to identify bioactive molecules with nutraceutical properties.
34509152	0	47	theme	molecular	71:79	arg1	approach					89:96	molecular network approach	71:96	molecular network approach	71:96	Dereplication of extracts from nutraceutical mushrooms Pleurotus using molecular network approach.
34509152	3	48	theme	lipids	488:493	arg1	they					435:438	they	435:438	they	435:438	Recently, Pleurotus has become popular as a functional food owing to its health benefits, primarily because they are a source of vitamins, fibers, minerals, and lipids.
34509152	3	48	theme	lipids	488:493	arg1	source					446:451	a source	444:451	a source of vitamins, fibers, minerals, and lipids	444:493	Recently, Pleurotus has become popular as a functional food owing to its health benefits, primarily because they are a source of vitamins, fibers, minerals, and lipids.
33309373	6	0	theme	survival	603:610	arg1	higher					684:689	higher	684:689	higher	684:689	We found that the survival rate of Lactiplantibacillus plantarum AR113 after freeze-drying was 19% higher in the presence of soy polysaccharides than with trehalose, the best-performing micromolecular cryoprotectant.
33309373	6	0	theme	survival	603:610	arg1	rate					612:615	the survival rate	599:615	the survival rate of Lactiplantibacillus plantarum AR113 after freeze-drying	599:674	We found that the survival rate of Lactiplantibacillus plantarum AR113 after freeze-drying was 19% higher in the presence of soy polysaccharides than with trehalose, the best-performing micromolecular cryoprotectant.
33309373	8	1	theme	macromolecular	1189:1202	arg1	cryoprotectants					1223:1237	macromolecular and micromolecular cryoprotectants	1189:1237	macromolecular and micromolecular cryoprotectants	1189:1237	These results demonstrate that macromolecular and micromolecular cryoprotectants have similar effects, and that combinations of macromolecular and micromolecular cryoprotectants have better protective effects.
33309373	9	2	theme	composite	1300:1308	arg1	cryoprotectant					1310:1323	the composite cryoprotectant	1296:1323	the composite cryoprotectant	1296:1323	We further observed that the composite cryoprotectant can increase Lactobacilli survival by improving cell membrane integrity and lactate dehydrogenase activity.
33309373	1	3	theme	bacteria	160:167	arg1	preservation					169:180	bacteria preservation	160:180	bacteria preservation	160:180	Freeze-drying is one of the most commonly used methods of bacteria preservation.
33309373	1	4	theme	preservation	169:180	arg1	methods					149:155	the most commonly used methods	126:155	the most commonly used methods of bacteria preservation	126:180	Freeze-drying is one of the most commonly used methods of bacteria preservation.
33309373	7	5	theme	90.52	814:818	arg1	%					819:819	%	819:819	%	819:819	Moreover, a 90.52% survival rate of L. plantarum WCFS1 was achieved using the composite cryoprotectant containing soy polysaccharide and trehalose, which increased by 31.48 and 36.47% compared with adding solely trehalose or soy polysaccharide, respectively.
33309373	8	6	theme	similar	1147:1153	arg1	effects					1155:1161	similar effects	1147:1161	similar effects	1147:1161	These results demonstrate that macromolecular and micromolecular cryoprotectants have similar effects, and that combinations of macromolecular and micromolecular cryoprotectants have better protective effects.
33309373	7	7	theme	soy	1027:1029	arg1	polysaccharide					1031:1044	soy polysaccharide	1027:1044	soy polysaccharide	1027:1044	Moreover, a 90.52% survival rate of L. plantarum WCFS1 was achieved using the composite cryoprotectant containing soy polysaccharide and trehalose, which increased by 31.48 and 36.47% compared with adding solely trehalose or soy polysaccharide, respectively.
33309373	10	8	theme	new	1456:1458	arg1	safer					1491:1495	safer	1491:1495	safer	1491:1495	Our finding provides a new type of cryoprotectant that is safer and more effective, which can be extensively applied in the relevant food industry.
33309373	10	8	theme	new	1456:1458	arg1	type					1460:1463	a new type	1454:1463	a new type of cryoprotectant that is safer and more effective, which can be extensively applied in the relevant food industry	1454:1578	Our finding provides a new type of cryoprotectant that is safer and more effective, which can be extensively applied in the relevant food industry.
33309373	8	9	contain	have	1239:1242	arg2	effects					1262:1268	better protective effects	1244:1268	better protective effects	1244:1268	These results demonstrate that macromolecular and micromolecular cryoprotectants have similar effects, and that combinations of macromolecular and micromolecular cryoprotectants have better protective effects.
33309373	8	9	contain	have	1239:1242	arg1	combinations					1173:1184	combinations	1173:1184	combinations of macromolecular and micromolecular cryoprotectants	1173:1237	These results demonstrate that macromolecular and micromolecular cryoprotectants have similar effects, and that combinations of macromolecular and micromolecular cryoprotectants have better protective effects.
33309373	9	10	theme	Lactobacilli	1338:1349	arg1	survival					1351:1358	Lactobacilli survival	1338:1358	Lactobacilli survival	1338:1358	We further observed that the composite cryoprotectant can increase Lactobacilli survival by improving cell membrane integrity and lactate dehydrogenase activity.
33309373	6	11	theme	soy	710:712	arg1	polysaccharides					714:728	soy polysaccharides	710:728	soy polysaccharides	710:728	We found that the survival rate of Lactiplantibacillus plantarum AR113 after freeze-drying was 19% higher in the presence of soy polysaccharides than with trehalose, the best-performing micromolecular cryoprotectant.
33309373	4	12	theme	cryoprotectants	407:421	arg1	types					398:402	other types	392:402	other types of cryoprotectants	392:421	Therefore, explorations of other types of cryoprotectants are needed.
33309373	5	13	theme	bacterial	565:573	arg1	activity					575:582	bacterial activity	565:582	bacterial activity	565:582	This study aimed to explore the possibility of the macromolecular cryoprotectants and combinations of cryoprotectants to maintain bacterial activity.
33309373	3	14	theme	Micromolecular	256:269	arg1	cryoprotectants					271:285	Micromolecular cryoprotectants	256:285	Micromolecular cryoprotectants	256:285	Micromolecular cryoprotectants have been widely adopted but have limited selectivity and protective effects.
33309373	10	15	theme	food	1566:1569	arg1	industry					1571:1578	the relevant food industry	1553:1578	the relevant food industry	1553:1578	Our finding provides a new type of cryoprotectant that is safer and more effective, which can be extensively applied in the relevant food industry.
33309373	6	16	theme	best-performing	755:769	arg1	cryoprotectant					786:799	the best-performing micromolecular cryoprotectant	751:799	the best-performing micromolecular cryoprotectant	751:799	We found that the survival rate of Lactiplantibacillus plantarum AR113 after freeze-drying was 19% higher in the presence of soy polysaccharides than with trehalose, the best-performing micromolecular cryoprotectant.
33309373	6	16	theme	best-performing	755:769	arg1	trehalose					740:748	trehalose	740:748	trehalose	740:748	We found that the survival rate of Lactiplantibacillus plantarum AR113 after freeze-drying was 19% higher in the presence of soy polysaccharides than with trehalose, the best-performing micromolecular cryoprotectant.
33309373	8	17	theme	cryoprotectants	1223:1237	arg1	combinations					1173:1184	combinations	1173:1184	combinations of macromolecular and micromolecular cryoprotectants	1173:1237	These results demonstrate that macromolecular and micromolecular cryoprotectants have similar effects, and that combinations of macromolecular and micromolecular cryoprotectants have better protective effects.
33309373	8	18	theme	micromolecular	1111:1124	arg1	cryoprotectants					1126:1140	macromolecular and micromolecular cryoprotectants	1092:1140	macromolecular and micromolecular cryoprotectants	1092:1140	These results demonstrate that macromolecular and micromolecular cryoprotectants have similar effects, and that combinations of macromolecular and micromolecular cryoprotectants have better protective effects.
33309373	7	19	theme	soy	916:918	arg1	polysaccharide					920:933	soy polysaccharide	916:933	soy polysaccharide	916:933	Moreover, a 90.52% survival rate of L. plantarum WCFS1 was achieved using the composite cryoprotectant containing soy polysaccharide and trehalose, which increased by 31.48 and 36.47% compared with adding solely trehalose or soy polysaccharide, respectively.
33309373	7	20	theme	composite	880:888	arg1	cryoprotectant					890:903	the composite cryoprotectant	876:903	the composite cryoprotectant containing soy polysaccharide and trehalose, which increased by 31.48 and 36.47% compared with adding solely trehalose or soy polysaccharide, respectively	876:1058	Moreover, a 90.52% survival rate of L. plantarum WCFS1 was achieved using the composite cryoprotectant containing soy polysaccharide and trehalose, which increased by 31.48 and 36.47% compared with adding solely trehalose or soy polysaccharide, respectively.
33309373	7	21	contain	containing	905:914	arg1	cryoprotectant					890:903	the composite cryoprotectant	876:903	the composite cryoprotectant containing soy polysaccharide and trehalose, which increased by 31.48 and 36.47% compared with adding solely trehalose or soy polysaccharide, respectively	876:1058	Moreover, a 90.52% survival rate of L. plantarum WCFS1 was achieved using the composite cryoprotectant containing soy polysaccharide and trehalose, which increased by 31.48 and 36.47% compared with adding solely trehalose or soy polysaccharide, respectively.
33309373	7	21	contain	containing	905:914	arg2	polysaccharide					920:933	soy polysaccharide	916:933	soy polysaccharide	916:933	Moreover, a 90.52% survival rate of L. plantarum WCFS1 was achieved using the composite cryoprotectant containing soy polysaccharide and trehalose, which increased by 31.48 and 36.47% compared with adding solely trehalose or soy polysaccharide, respectively.
33309373	7	21	contain	containing	905:914	arg2	trehalose					939:947	trehalose	939:947	trehalose	939:947	Moreover, a 90.52% survival rate of L. plantarum WCFS1 was achieved using the composite cryoprotectant containing soy polysaccharide and trehalose, which increased by 31.48 and 36.47% compared with adding solely trehalose or soy polysaccharide, respectively.
33309373	9	22	theme	cell	1373:1376	arg1	integrity					1387:1395	cell membrane integrity	1373:1395	cell membrane integrity	1373:1395	We further observed that the composite cryoprotectant can increase Lactobacilli survival by improving cell membrane integrity and lactate dehydrogenase activity.
33309373	10	23	theme	cryoprotectant	1468:1481	arg1	safer					1491:1495	safer	1491:1495	safer	1491:1495	Our finding provides a new type of cryoprotectant that is safer and more effective, which can be extensively applied in the relevant food industry.
33309373	10	23	theme	cryoprotectant	1468:1481	arg1	type					1460:1463	a new type	1454:1463	a new type of cryoprotectant that is safer and more effective, which can be extensively applied in the relevant food industry	1454:1578	Our finding provides a new type of cryoprotectant that is safer and more effective, which can be extensively applied in the relevant food industry.
33309373	9	24	theme	membrane	1378:1385	arg1	integrity					1387:1395	cell membrane integrity	1373:1395	cell membrane integrity	1373:1395	We further observed that the composite cryoprotectant can increase Lactobacilli survival by improving cell membrane integrity and lactate dehydrogenase activity.
33309373	3	25	contain	have	316:319	arg1	cryoprotectants					271:285	Micromolecular cryoprotectants	256:285	Micromolecular cryoprotectants	256:285	Micromolecular cryoprotectants have been widely adopted but have limited selectivity and protective effects.
33309373	3	25	contain	have	316:319	arg2	selectivity					329:339	limited selectivity	321:339	limited selectivity	321:339	Micromolecular cryoprotectants have been widely adopted but have limited selectivity and protective effects.
33309373	3	25	contain	have	316:319	arg2	effects					356:362	protective effects	345:362	protective effects	345:362	Micromolecular cryoprotectants have been widely adopted but have limited selectivity and protective effects.
33309373	8	26	theme	micromolecular	1208:1221	arg1	cryoprotectants					1223:1237	macromolecular and micromolecular cryoprotectants	1189:1237	macromolecular and micromolecular cryoprotectants	1189:1237	These results demonstrate that macromolecular and micromolecular cryoprotectants have similar effects, and that combinations of macromolecular and micromolecular cryoprotectants have better protective effects.
33309373	5	27	theme	cryoprotectants	537:551	arg1	combinations					521:532	combinations	521:532	combinations of cryoprotectants	521:551	This study aimed to explore the possibility of the macromolecular cryoprotectants and combinations of cryoprotectants to maintain bacterial activity.
33309373	6	28	theme	AR113	650:654	arg1	higher					684:689	higher	684:689	higher	684:689	We found that the survival rate of Lactiplantibacillus plantarum AR113 after freeze-drying was 19% higher in the presence of soy polysaccharides than with trehalose, the best-performing micromolecular cryoprotectant.
33309373	6	28	theme	AR113	650:654	arg1	rate					612:615	the survival rate	599:615	the survival rate of Lactiplantibacillus plantarum AR113 after freeze-drying	599:674	We found that the survival rate of Lactiplantibacillus plantarum AR113 after freeze-drying was 19% higher in the presence of soy polysaccharides than with trehalose, the best-performing micromolecular cryoprotectant.
33309373	7	29	theme	WCFS1	851:855	arg1	rate					830:833	a 90.52% survival rate	812:833	a 90.52% survival rate of L. plantarum WCFS1	812:855	Moreover, a 90.52% survival rate of L. plantarum WCFS1 was achieved using the composite cryoprotectant containing soy polysaccharide and trehalose, which increased by 31.48 and 36.47% compared with adding solely trehalose or soy polysaccharide, respectively.
33309373	8	30	contain	have	1142:1145	arg1	cryoprotectants					1126:1140	macromolecular and micromolecular cryoprotectants	1092:1140	macromolecular and micromolecular cryoprotectants	1092:1140	These results demonstrate that macromolecular and micromolecular cryoprotectants have similar effects, and that combinations of macromolecular and micromolecular cryoprotectants have better protective effects.
33309373	8	30	contain	have	1142:1145	arg2	effects					1155:1161	similar effects	1147:1161	similar effects	1147:1161	These results demonstrate that macromolecular and micromolecular cryoprotectants have similar effects, and that combinations of macromolecular and micromolecular cryoprotectants have better protective effects.
33309373	6	31	theme	plantarum	640:648	arg1	AR113					650:654	Lactiplantibacillus plantarum AR113	620:654	Lactiplantibacillus plantarum AR113	620:654	We found that the survival rate of Lactiplantibacillus plantarum AR113 after freeze-drying was 19% higher in the presence of soy polysaccharides than with trehalose, the best-performing micromolecular cryoprotectant.
33309373	9	32	theme	lactate	1401:1407	arg1	activity					1423:1430	lactate dehydrogenase activity	1401:1430	lactate dehydrogenase activity	1401:1430	We further observed that the composite cryoprotectant can increase Lactobacilli survival by improving cell membrane integrity and lactate dehydrogenase activity.
33309373	2	33	theme	cellular	239:246	arg1	damage					248:253	cellular damage	239:253	cellular damage	239:253	During this process, cryoprotectants can greatly reduce cellular damage.
33309373	8	34	theme	protective	1251:1260	arg1	effects					1262:1268	better protective effects	1244:1268	better protective effects	1244:1268	These results demonstrate that macromolecular and micromolecular cryoprotectants have similar effects, and that combinations of macromolecular and micromolecular cryoprotectants have better protective effects.
33309373	6	35	theme	Lactiplantibacillus	620:638	arg1	AR113					650:654	Lactiplantibacillus plantarum AR113	620:654	Lactiplantibacillus plantarum AR113	620:654	We found that the survival rate of Lactiplantibacillus plantarum AR113 after freeze-drying was 19% higher in the presence of soy polysaccharides than with trehalose, the best-performing micromolecular cryoprotectant.
33309373	7	36	theme	L.	838:839	arg1	WCFS1					851:855	L. plantarum WCFS1	838:855	L. plantarum WCFS1	838:855	Moreover, a 90.52% survival rate of L. plantarum WCFS1 was achieved using the composite cryoprotectant containing soy polysaccharide and trehalose, which increased by 31.48 and 36.47% compared with adding solely trehalose or soy polysaccharide, respectively.
33309373	3	37	theme	protective	345:354	arg1	effects					356:362	protective effects	345:362	protective effects	345:362	Micromolecular cryoprotectants have been widely adopted but have limited selectivity and protective effects.
33309373	9	38	theme	dehydrogenase	1409:1421	arg1	activity					1423:1430	lactate dehydrogenase activity	1401:1430	lactate dehydrogenase activity	1401:1430	We further observed that the composite cryoprotectant can increase Lactobacilli survival by improving cell membrane integrity and lactate dehydrogenase activity.
33309373	10	39	theme	relevant	1557:1564	arg1	industry					1571:1578	the relevant food industry	1553:1578	the relevant food industry	1553:1578	Our finding provides a new type of cryoprotectant that is safer and more effective, which can be extensively applied in the relevant food industry.
33309373	4	40	theme	types	398:402	arg1	explorations					376:387	explorations	376:387	explorations of other types of cryoprotectants	376:421	Therefore, explorations of other types of cryoprotectants are needed.
33309373	7	41	theme	plantarum	841:849	arg1	WCFS1					851:855	L. plantarum WCFS1	838:855	L. plantarum WCFS1	838:855	Moreover, a 90.52% survival rate of L. plantarum WCFS1 was achieved using the composite cryoprotectant containing soy polysaccharide and trehalose, which increased by 31.48 and 36.47% compared with adding solely trehalose or soy polysaccharide, respectively.
33309373	3	42	theme	limited	321:327	arg1	selectivity					329:339	limited selectivity	321:339	limited selectivity	321:339	Micromolecular cryoprotectants have been widely adopted but have limited selectivity and protective effects.
33309373	8	43	theme	macromolecular	1092:1105	arg1	cryoprotectants					1126:1140	macromolecular and micromolecular cryoprotectants	1092:1140	macromolecular and micromolecular cryoprotectants	1092:1140	These results demonstrate that macromolecular and micromolecular cryoprotectants have similar effects, and that combinations of macromolecular and micromolecular cryoprotectants have better protective effects.
33309373	4	44	theme	other	392:396	arg1	types					398:402	other types	392:402	other types of cryoprotectants	392:421	Therefore, explorations of other types of cryoprotectants are needed.
33309373	6	45	theme	polysaccharides	714:728	arg1	presence					698:705	the presence	694:705	the presence of soy polysaccharides	694:728	We found that the survival rate of Lactiplantibacillus plantarum AR113 after freeze-drying was 19% higher in the presence of soy polysaccharides than with trehalose, the best-performing micromolecular cryoprotectant.
33309373	8	46	theme	better	1244:1249	arg1	effects					1262:1268	better protective effects	1244:1268	better protective effects	1244:1268	These results demonstrate that macromolecular and micromolecular cryoprotectants have similar effects, and that combinations of macromolecular and micromolecular cryoprotectants have better protective effects.
33309373	1	47	theme	used	144:147	arg1	methods					149:155	the most commonly used methods	126:155	the most commonly used methods of bacteria preservation	126:180	Freeze-drying is one of the most commonly used methods of bacteria preservation.
33309373	5	48	theme	macromolecular	486:499	arg1	cryoprotectants					501:515	the macromolecular cryoprotectants	482:515	the macromolecular cryoprotectants	482:515	This study aimed to explore the possibility of the macromolecular cryoprotectants and combinations of cryoprotectants to maintain bacterial activity.
33309373	6	49	theme	micromolecular	771:784	arg1	cryoprotectant					786:799	the best-performing micromolecular cryoprotectant	751:799	the best-performing micromolecular cryoprotectant	751:799	We found that the survival rate of Lactiplantibacillus plantarum AR113 after freeze-drying was 19% higher in the presence of soy polysaccharides than with trehalose, the best-performing micromolecular cryoprotectant.
33309373	6	49	theme	micromolecular	771:784	arg1	trehalose					740:748	trehalose	740:748	trehalose	740:748	We found that the survival rate of Lactiplantibacillus plantarum AR113 after freeze-drying was 19% higher in the presence of soy polysaccharides than with trehalose, the best-performing micromolecular cryoprotectant.
33309373	1	50	theme	methods	149:155	arg1	one					119:121	one	119:121	one	119:121	Freeze-drying is one of the most commonly used methods of bacteria preservation.
33309373	1	50	theme	methods	149:155	arg1	methods					149:155	the most commonly used methods	126:155	the most commonly used methods of bacteria preservation	126:180	Freeze-drying is one of the most commonly used methods of bacteria preservation.
33309373	5	51	theme	cryoprotectants	501:515	arg1	possibility					467:477	the possibility	463:477	the possibility of the macromolecular cryoprotectants	463:515	This study aimed to explore the possibility of the macromolecular cryoprotectants and combinations of cryoprotectants to maintain bacterial activity.
33309373	7	52	theme	%	819:819	arg1	rate					830:833	a 90.52% survival rate	812:833	a 90.52% survival rate of L. plantarum WCFS1	812:855	Moreover, a 90.52% survival rate of L. plantarum WCFS1 was achieved using the composite cryoprotectant containing soy polysaccharide and trehalose, which increased by 31.48 and 36.47% compared with adding solely trehalose or soy polysaccharide, respectively.
33309373	0	53	theme	plantarum	64:72	arg1	survival					32:39	the survival	28:39	the survival of Lactiplantibacillus plantarum subjected to freeze-drying	28:99	Polysaccharides can improve the survival of Lactiplantibacillus plantarum subjected to freeze-drying.
33309373	7	54	theme	survival	821:828	arg1	rate					830:833	a 90.52% survival rate	812:833	a 90.52% survival rate of L. plantarum WCFS1	812:855	Moreover, a 90.52% survival rate of L. plantarum WCFS1 was achieved using the composite cryoprotectant containing soy polysaccharide and trehalose, which increased by 31.48 and 36.47% compared with adding solely trehalose or soy polysaccharide, respectively.
32599235	0	0	theme	gum	72:74	arg1	validation					11:20	validation	11:20	validation	11:20	Design and validation of antibacterial and pH response of cationic guar gum film by combining hydroxyethyl cellulose and red cabbage pigment.
32599235	0	0	theme	gum	72:74	arg1	Design					0:5	Design	0:5	Design	0:5	Design and validation of antibacterial and pH response of cationic guar gum film by combining hydroxyethyl cellulose and red cabbage pigment.
32599235	1	1	dep	composite	257:265	arg1	response					221:228	pH response	218:228	pH response of cationic guar gum	218:249	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	2	2	theme	tensile	506:512	arg1	51.59 MPa					524:532	51.59 MPa	524:532	51.59 MPa	524:532	The effect of different HEC content on the binary composite films (CGG-HEC) performance shows that the highest tensile strength (51.59 MPa) can be obtained by adding 10% HEC due to the good compatibility between CGG and HEC.
32599235	2	2	theme	tensile	506:512	arg1	strength					514:521	the highest tensile strength	494:521	the highest tensile strength (51.59 MPa)	494:533	The effect of different HEC content on the binary composite films (CGG-HEC) performance shows that the highest tensile strength (51.59 MPa) can be obtained by adding 10% HEC due to the good compatibility between CGG and HEC.
32599235	3	3	theme	transmission	789:800	arg1	1.08					815:818	1.08	815:818	1.08	815:818	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	3	3	theme	transmission	789:800	arg1	coefficient					802:812	appropriate water vapor transmission coefficient	765:812	appropriate water vapor transmission coefficient (1.08)	765:819	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	1	4	theme	hydroxyethyl	301:312	arg1	cellulose					314:322	hydroxyethyl cellulose	301:322	hydroxyethyl cellulose (HEC)	301:328	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	1	4	theme	hydroxyethyl	301:312	arg1	enhancer					336:343	an enhancer	333:343	an enhancer	333:343	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	1	4	theme	hydroxyethyl	301:312	arg1	substance					384:392	a smart active substance	369:392	a smart active substance	369:392	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	1	4	theme	hydroxyethyl	301:312	arg1	HEC					325:327	HEC	325:327	HEC	325:327	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	3	5	theme	3	670:670	arg1	%					671:671	%	671:671	%	671:671	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	0	6	theme	guar	67:70	arg1	gum					72:74	cationic guar gum film	58:79	cationic guar gum film by combining hydroxyethyl cellulose and red cabbage pigment	58:139	Design and validation of antibacterial and pH response of cationic guar gum film by combining hydroxyethyl cellulose and red cabbage pigment.
32599235	3	7	theme	appropriate	765:775	arg1	vapor					783:787	appropriate water vapor	765:787	appropriate water vapor transmission coefficient (1.08)	765:819	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	2	8	theme	highest	498:504	arg1	51.59 MPa					524:532	51.59 MPa	524:532	51.59 MPa	524:532	The effect of different HEC content on the binary composite films (CGG-HEC) performance shows that the highest tensile strength (51.59 MPa) can be obtained by adding 10% HEC due to the good compatibility between CGG and HEC.
32599235	2	8	theme	highest	498:504	arg1	strength					514:521	the highest tensile strength	494:521	the highest tensile strength (51.59 MPa)	494:533	The effect of different HEC content on the binary composite films (CGG-HEC) performance shows that the highest tensile strength (51.59 MPa) can be obtained by adding 10% HEC due to the good compatibility between CGG and HEC.
32599235	1	9	theme	simple	144:149	arg1	method					164:169	A simple and feasible method	142:169	A simple and feasible method	142:169	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	1	10	theme	cationic	233:240	arg1	gum					247:249	cationic guar gum	233:249	cationic guar gum	233:249	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	4	11	theme	RC	1010:1011	arg1	properties					988:997	the antibacterial properties	970:997	the antibacterial properties of CGG and RC	970:1011	In addition, RC3 has good antibacterial properties against E. coli and Staphylococcus aureus, taking advantage of the antibacterial properties of CGG and RC.
32599235	3	12	with	film	642:645	arg1	addition					676:683	3% RC addition	670:683	3% RC addition	670:683	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	3	12	with	film	642:645	arg1	HEC					662:664	HEC	662:664	HEC	662:664	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	6	13	contain	has	1236:1238	arg2	antibacterial					1268:1280	antibacterial	1268:1280	antibacterial	1268:1280	Therefore, the ternary composite film (RC3) has good mechanical properties, antibacterial and intelligent response characteristics, and may be used in intelligent antibacterial packaging.
32599235	6	13	contain	has	1236:1238	arg1	RC3					1231:1233	RC3	1231:1233	RC3	1231:1233	Therefore, the ternary composite film (RC3) has good mechanical properties, antibacterial and intelligent response characteristics, and may be used in intelligent antibacterial packaging.
32599235	6	13	contain	has	1236:1238	arg2	properties					1256:1265	good mechanical properties	1240:1265	good mechanical properties	1240:1265	Therefore, the ternary composite film (RC3) has good mechanical properties, antibacterial and intelligent response characteristics, and may be used in intelligent antibacterial packaging.
32599235	6	13	contain	has	1236:1238	arg2	characteristics					1307:1321	intelligent response characteristics	1286:1321	intelligent response characteristics	1286:1321	Therefore, the ternary composite film (RC3) has good mechanical properties, antibacterial and intelligent response characteristics, and may be used in intelligent antibacterial packaging.
32599235	6	13	contain	has	1236:1238	arg1	film					1225:1228	the ternary composite film	1203:1228	the ternary composite film (RC3)	1203:1234	Therefore, the ternary composite film (RC3) has good mechanical properties, antibacterial and intelligent response characteristics, and may be used in intelligent antibacterial packaging.
32599235	1	14	theme	guar	242:245	arg1	gum					247:249	cationic guar gum	233:249	cationic guar gum	233:249	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	1	15	dep	CGG-HEC	274:280	arg1	RC					283:284	RC	283:284	RC	283:284	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	2	16	theme	binary	438:443	arg1	performance					471:481	the binary composite films (CGG-HEC) performance	434:481	the binary composite films (CGG-HEC) performance	434:481	The effect of different HEC content on the binary composite films (CGG-HEC) performance shows that the highest tensile strength (51.59 MPa) can be obtained by adding 10% HEC due to the good compatibility between CGG and HEC.
32599235	1	17	theme	gum	247:249	arg1	response					221:228	pH response	218:228	pH response of cationic guar gum	218:249	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	0	18	theme	hydroxyethyl	94:105	arg1	cellulose					107:115	hydroxyethyl cellulose	94:115	hydroxyethyl cellulose	94:115	Design and validation of antibacterial and pH response of cationic guar gum film by combining hydroxyethyl cellulose and red cabbage pigment.
32599235	6	19	theme	antibacterial	1355:1367	arg1	packaging					1369:1377	intelligent antibacterial packaging	1343:1377	intelligent antibacterial packaging	1343:1377	Therefore, the ternary composite film (RC3) has good mechanical properties, antibacterial and intelligent response characteristics, and may be used in intelligent antibacterial packaging.
32599235	3	20	theme	high	730:733	arg1	strength					743:750	high tensile strength	730:750	high tensile strength (65.41 MPa)	730:762	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	3	20	theme	high	730:733	arg1	65.41 MPa					753:761	65.41 MPa	753:761	65.41 MPa	753:761	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	2	21	theme	HEC	419:421	arg1	content					423:429	different HEC content	409:429	different HEC content	409:429	The effect of different HEC content on the binary composite films (CGG-HEC) performance shows that the highest tensile strength (51.59 MPa) can be obtained by adding 10% HEC due to the good compatibility between CGG and HEC.
32599235	1	22	theme	smart	371:375	arg1	cellulose					314:322	hydroxyethyl cellulose	301:322	hydroxyethyl cellulose (HEC)	301:328	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	1	22	theme	smart	371:375	arg1	substance					384:392	a smart active substance	369:392	a smart active substance	369:392	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	5	23	theme	significant	1071:1081	arg1	change					1089:1094	a significant color change	1069:1094	a significant color change	1069:1094	RC3 can respond to changes in environmental pH and has a significant color change, and also has a significant color change when detecting the deterioration of pork and soy milk.
32599235	5	24	theme	color	1124:1128	arg1	change					1130:1135	a significant color change	1110:1135	a significant color change	1110:1135	RC3 can respond to changes in environmental pH and has a significant color change, and also has a significant color change when detecting the deterioration of pork and soy milk.
32599235	1	25	theme	feasible	155:162	arg1	method					164:169	A simple and feasible method	142:169	A simple and feasible method	142:169	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	4	26	theme	antibacterial	974:986	arg1	properties					988:997	the antibacterial properties	970:997	the antibacterial properties of CGG and RC	970:1011	In addition, RC3 has good antibacterial properties against E. coli and Staphylococcus aureus, taking advantage of the antibacterial properties of CGG and RC.
32599235	3	27	theme	%	671:671	arg1	addition					676:683	3% RC addition	670:683	3% RC addition	670:683	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	1	28	theme	active	377:382	arg1	cellulose					314:322	hydroxyethyl cellulose	301:322	hydroxyethyl cellulose (HEC)	301:328	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	1	28	theme	active	377:382	arg1	substance					384:392	a smart active substance	369:392	a smart active substance	369:392	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	5	29	contain	has	1106:1108	arg1	RC3					1014:1016	RC3	1014:1016	RC3	1014:1016	RC3 can respond to changes in environmental pH and has a significant color change, and also has a significant color change when detecting the deterioration of pork and soy milk.
32599235	5	29	contain	has	1106:1108	arg2	change					1130:1135	a significant color change	1110:1135	a significant color change	1110:1135	RC3 can respond to changes in environmental pH and has a significant color change, and also has a significant color change when detecting the deterioration of pork and soy milk.
32599235	2	30	theme	composite	445:453	arg1	performance					471:481	the binary composite films (CGG-HEC) performance	434:481	the binary composite films (CGG-HEC) performance	434:481	The effect of different HEC content on the binary composite films (CGG-HEC) performance shows that the highest tensile strength (51.59 MPa) can be obtained by adding 10% HEC due to the good compatibility between CGG and HEC.
32599235	3	31	contain	has	685:687	arg1	film					642:645	The ternary composite film	620:645	The ternary composite film (RC3) with 10% HEC and 3% RC addition	620:683	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	3	31	contain	has	685:687	arg2	performance					694:704	good performance	689:704	good performance	689:704	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	3	31	contain	has	685:687	arg1	RC3					648:650	RC3	648:650	RC3	648:650	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	3	32	theme	good	826:829	arg1	stability					845:853	good thermodynamic stability	826:853	good thermodynamic stability	826:853	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	4	33	contain	has	873:875	arg2	properties					896:905	good antibacterial properties	877:905	good antibacterial properties	877:905	In addition, RC3 has good antibacterial properties against E. coli and Staphylococcus aureus, taking advantage of the antibacterial properties of CGG and RC.
32599235	4	33	contain	has	873:875	arg1	RC3					869:871	RC3	869:871	RC3	869:871	In addition, RC3 has good antibacterial properties against E. coli and Staphylococcus aureus, taking advantage of the antibacterial properties of CGG and RC.
32599235	6	34	theme	response	1298:1305	arg1	characteristics					1307:1321	intelligent response characteristics	1286:1321	intelligent response characteristics	1286:1321	Therefore, the ternary composite film (RC3) has good mechanical properties, antibacterial and intelligent response characteristics, and may be used in intelligent antibacterial packaging.
32599235	6	35	theme	ternary	1207:1213	arg1	RC3					1231:1233	RC3	1231:1233	RC3	1231:1233	Therefore, the ternary composite film (RC3) has good mechanical properties, antibacterial and intelligent response characteristics, and may be used in intelligent antibacterial packaging.
32599235	6	35	theme	ternary	1207:1213	arg1	film					1225:1228	the ternary composite film	1203:1228	the ternary composite film (RC3)	1203:1234	Therefore, the ternary composite film (RC3) has good mechanical properties, antibacterial and intelligent response characteristics, and may be used in intelligent antibacterial packaging.
32599235	3	36	theme	ternary	624:630	arg1	film					642:645	The ternary composite film	620:645	The ternary composite film (RC3) with 10% HEC and 3% RC addition	620:683	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	3	36	theme	ternary	624:630	arg1	RC3					648:650	RC3	648:650	RC3	648:650	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	3	37	theme	thermodynamic	831:843	arg1	stability					845:853	good thermodynamic stability	826:853	good thermodynamic stability	826:853	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	6	38	theme	intelligent	1286:1296	arg1	characteristics					1307:1321	intelligent response characteristics	1286:1321	intelligent response characteristics	1286:1321	Therefore, the ternary composite film (RC3) has good mechanical properties, antibacterial and intelligent response characteristics, and may be used in intelligent antibacterial packaging.
32599235	2	39	theme	films	455:459	arg1	performance					471:481	the binary composite films (CGG-HEC) performance	434:481	the binary composite films (CGG-HEC) performance	434:481	The effect of different HEC content on the binary composite films (CGG-HEC) performance shows that the highest tensile strength (51.59 MPa) can be obtained by adding 10% HEC due to the good compatibility between CGG and HEC.
32599235	2	40	from	effect	399:404	arg1	performance					471:481	the binary composite films (CGG-HEC) performance	434:481	the binary composite films (CGG-HEC) performance	434:481	The effect of different HEC content on the binary composite films (CGG-HEC) performance shows that the highest tensile strength (51.59 MPa) can be obtained by adding 10% HEC due to the good compatibility between CGG and HEC.
32599235	5	41	theme	milk	1186:1189	arg1	deterioration					1156:1168	the deterioration	1152:1168	the deterioration of pork and soy milk	1152:1189	RC3 can respond to changes in environmental pH and has a significant color change, and also has a significant color change when detecting the deterioration of pork and soy milk.
32599235	3	42	theme	tensile	735:741	arg1	strength					743:750	high tensile strength	730:750	high tensile strength (65.41 MPa)	730:762	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	3	42	theme	tensile	735:741	arg1	65.41 MPa					753:761	65.41 MPa	753:761	65.41 MPa	753:761	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	6	43	theme	mechanical	1245:1254	arg1	properties					1256:1265	good mechanical properties	1240:1265	good mechanical properties	1240:1265	Therefore, the ternary composite film (RC3) has good mechanical properties, antibacterial and intelligent response characteristics, and may be used in intelligent antibacterial packaging.
32599235	1	44	theme	composite	257:265	arg1	CGG-HEC					274:280	CGG-HEC	274:280	CGG-HEC	274:280	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	1	44	theme	composite	257:265	arg1	films					267:271	the antibacterial and pH response of cationic guar gum (CGG) composite films	196:271	the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC)	196:285	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	0	45	theme	cabbage	125:131	arg1	pigment					133:139	red cabbage pigment	121:139	red cabbage pigment	121:139	Design and validation of antibacterial and pH response of cationic guar gum film by combining hydroxyethyl cellulose and red cabbage pigment.
32599235	5	46	theme	pork	1173:1176	arg1	deterioration					1156:1168	the deterioration	1152:1168	the deterioration of pork and soy milk	1152:1189	RC3 can respond to changes in environmental pH and has a significant color change, and also has a significant color change when detecting the deterioration of pork and soy milk.
32599235	6	47	theme	good	1240:1243	arg1	properties					1256:1265	good mechanical properties	1240:1265	good mechanical properties	1240:1265	Therefore, the ternary composite film (RC3) has good mechanical properties, antibacterial and intelligent response characteristics, and may be used in intelligent antibacterial packaging.
32599235	0	48	theme	antibacterial	25:37	arg1	validation					11:20	validation	11:20	validation	11:20	Design and validation of antibacterial and pH response of cationic guar gum film by combining hydroxyethyl cellulose and red cabbage pigment.
32599235	0	48	theme	antibacterial	25:37	arg1	Design					0:5	Design	0:5	Design	0:5	Design and validation of antibacterial and pH response of cationic guar gum film by combining hydroxyethyl cellulose and red cabbage pigment.
32599235	0	49	theme	red	121:123	arg1	pigment					133:139	red cabbage pigment	121:139	red cabbage pigment	121:139	Design and validation of antibacterial and pH response of cationic guar gum film by combining hydroxyethyl cellulose and red cabbage pigment.
32599235	2	50	theme	good	580:583	arg1	compatibility					585:597	the good compatibility	576:597	the good compatibility between CGG and HEC	576:617	The effect of different HEC content on the binary composite films (CGG-HEC) performance shows that the highest tensile strength (51.59 MPa) can be obtained by adding 10% HEC due to the good compatibility between CGG and HEC.
32599235	0	51	theme	response	46:53	arg1	validation					11:20	validation	11:20	validation	11:20	Design and validation of antibacterial and pH response of cationic guar gum film by combining hydroxyethyl cellulose and red cabbage pigment.
32599235	0	51	theme	response	46:53	arg1	Design					0:5	Design	0:5	Design	0:5	Design and validation of antibacterial and pH response of cationic guar gum film by combining hydroxyethyl cellulose and red cabbage pigment.
32599235	2	52	theme	content	423:429	arg1	effect					399:404	The effect	395:404	The effect of different HEC content on the binary composite films (CGG-HEC) performance	395:481	The effect of different HEC content on the binary composite films (CGG-HEC) performance shows that the highest tensile strength (51.59 MPa) can be obtained by adding 10% HEC due to the good compatibility between CGG and HEC.
32599235	5	53	from	changes	1033:1039	arg1	pH					1058:1059	environmental pH	1044:1059	environmental pH	1044:1059	RC3 can respond to changes in environmental pH and has a significant color change, and also has a significant color change when detecting the deterioration of pork and soy milk.
32599235	3	54	theme	water	777:781	arg1	vapor					783:787	appropriate water vapor	765:787	appropriate water vapor transmission coefficient (1.08)	765:819	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	6	55	theme	composite	1215:1223	arg1	RC3					1231:1233	RC3	1231:1233	RC3	1231:1233	Therefore, the ternary composite film (RC3) has good mechanical properties, antibacterial and intelligent response characteristics, and may be used in intelligent antibacterial packaging.
32599235	6	55	theme	composite	1215:1223	arg1	film					1225:1228	the ternary composite film	1203:1228	the ternary composite film (RC3)	1203:1234	Therefore, the ternary composite film (RC3) has good mechanical properties, antibacterial and intelligent response characteristics, and may be used in intelligent antibacterial packaging.
32599235	0	56	theme	pH	43:44	arg1	response					46:53	pH response	43:53	pH response	43:53	Design and validation of antibacterial and pH response of cationic guar gum film by combining hydroxyethyl cellulose and red cabbage pigment.
32599235	3	57	theme	composite	632:640	arg1	film					642:645	The ternary composite film	620:645	The ternary composite film (RC3) with 10% HEC and 3% RC addition	620:683	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	3	57	theme	composite	632:640	arg1	RC3					648:650	RC3	648:650	RC3	648:650	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	4	58	theme	properties	988:997	arg1	advantage					957:965	advantage	957:965	advantage of the antibacterial properties of CGG and RC	957:1011	In addition, RC3 has good antibacterial properties against E. coli and Staphylococcus aureus, taking advantage of the antibacterial properties of CGG and RC.
32599235	6	59	used	used	1335:1338	arg2	film					1225:1228	the ternary composite film	1203:1228	the ternary composite film (RC3)	1203:1234	Therefore, the ternary composite film (RC3) has good mechanical properties, antibacterial and intelligent response characteristics, and may be used in intelligent antibacterial packaging.
32599235	6	59	used	used	1335:1338	arg2	RC3					1231:1233	RC3	1231:1233	RC3	1231:1233	Therefore, the ternary composite film (RC3) has good mechanical properties, antibacterial and intelligent response characteristics, and may be used in intelligent antibacterial packaging.
32599235	4	60	theme	good	877:880	arg1	properties					896:905	good antibacterial properties	877:905	good antibacterial properties	877:905	In addition, RC3 has good antibacterial properties against E. coli and Staphylococcus aureus, taking advantage of the antibacterial properties of CGG and RC.
32599235	1	61	theme	red	349:351	arg1	RC					362:363	RC	362:363	RC	362:363	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	1	61	theme	red	349:351	arg1	cabbage					353:359	red cabbage	349:359	red cabbage (RC)	349:364	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	5	62	contain	has	1065:1067	arg1	RC3					1014:1016	RC3	1014:1016	RC3	1014:1016	RC3 can respond to changes in environmental pH and has a significant color change, and also has a significant color change when detecting the deterioration of pork and soy milk.
32599235	5	62	contain	has	1065:1067	arg2	change					1089:1094	a significant color change	1069:1094	a significant color change	1069:1094	RC3 can respond to changes in environmental pH and has a significant color change, and also has a significant color change when detecting the deterioration of pork and soy milk.
32599235	4	63	theme	CGG	1002:1004	arg1	properties					988:997	the antibacterial properties	970:997	the antibacterial properties of CGG and RC	970:1011	In addition, RC3 has good antibacterial properties against E. coli and Staphylococcus aureus, taking advantage of the antibacterial properties of CGG and RC.
32599235	3	64	theme	good	689:692	arg1	performance					694:704	good performance	689:704	good performance	689:704	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	1	65	theme	antibacterial	200:212	arg1	CGG-HEC					274:280	CGG-HEC	274:280	CGG-HEC	274:280	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	1	65	theme	antibacterial	200:212	arg1	films					267:271	the antibacterial and pH response of cationic guar gum (CGG) composite films	196:271	the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC)	196:285	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	2	66	theme	CGG-HEC	462:468	arg1	performance					471:481	the binary composite films (CGG-HEC) performance	434:481	the binary composite films (CGG-HEC) performance	434:481	The effect of different HEC content on the binary composite films (CGG-HEC) performance shows that the highest tensile strength (51.59 MPa) can be obtained by adding 10% HEC due to the good compatibility between CGG and HEC.
32599235	2	67	theme	different	409:417	arg1	content					423:429	different HEC content	409:429	different HEC content	409:429	The effect of different HEC content on the binary composite films (CGG-HEC) performance shows that the highest tensile strength (51.59 MPa) can be obtained by adding 10% HEC due to the good compatibility between CGG and HEC.
32599235	6	68	theme	intelligent	1343:1353	arg1	packaging					1369:1377	intelligent antibacterial packaging	1343:1377	intelligent antibacterial packaging	1343:1377	Therefore, the ternary composite film (RC3) has good mechanical properties, antibacterial and intelligent response characteristics, and may be used in intelligent antibacterial packaging.
32599235	3	69	theme	RC	673:674	arg1	addition					676:683	3% RC addition	670:683	3% RC addition	670:683	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	0	70	theme	cationic	58:65	arg1	gum					72:74	cationic guar gum film	58:79	cationic guar gum film by combining hydroxyethyl cellulose and red cabbage pigment	58:139	Design and validation of antibacterial and pH response of cationic guar gum film by combining hydroxyethyl cellulose and red cabbage pigment.
32599235	5	71	theme	environmental	1044:1056	arg1	pH					1058:1059	environmental pH	1044:1059	environmental pH	1044:1059	RC3 can respond to changes in environmental pH and has a significant color change, and also has a significant color change when detecting the deterioration of pork and soy milk.
32599235	4	72	theme	antibacterial	882:894	arg1	properties					896:905	good antibacterial properties	877:905	good antibacterial properties	877:905	In addition, RC3 has good antibacterial properties against E. coli and Staphylococcus aureus, taking advantage of the antibacterial properties of CGG and RC.
32599235	1	73	theme	pH	218:219	arg1	response					221:228	pH response	218:228	pH response of cationic guar gum	218:249	A simple and feasible method was adopted to construct the antibacterial and pH response of cationic guar gum (CGG) composite films (CGG-HEC, RC) through using hydroxyethyl cellulose (HEC) as an enhancer and red cabbage (RC) as a smart active substance.
32599235	5	74	theme	significant	1112:1122	arg1	change					1130:1135	a significant color change	1110:1135	a significant color change	1110:1135	RC3 can respond to changes in environmental pH and has a significant color change, and also has a significant color change when detecting the deterioration of pork and soy milk.
32599235	3	75	theme	vapor	783:787	arg1	1.08					815:818	1.08	815:818	1.08	815:818	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	3	75	theme	vapor	783:787	arg1	coefficient					802:812	appropriate water vapor transmission coefficient	765:812	appropriate water vapor transmission coefficient (1.08)	765:819	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	3	76	theme	10	658:659	arg1	%					660:660	%	660:660	%	660:660	The ternary composite film (RC3) with 10% HEC and 3% RC addition has good performance in all aspects, such as high tensile strength (65.41 MPa), appropriate water vapor transmission coefficient (1.08), and good thermodynamic stability.
32599235	5	77	theme	color	1083:1087	arg1	change					1089:1094	a significant color change	1069:1094	a significant color change	1069:1094	RC3 can respond to changes in environmental pH and has a significant color change, and also has a significant color change when detecting the deterioration of pork and soy milk.
32599235	5	78	theme	soy	1182:1184	arg1	milk					1186:1189	soy milk	1182:1189	soy milk	1182:1189	RC3 can respond to changes in environmental pH and has a significant color change, and also has a significant color change when detecting the deterioration of pork and soy milk.
32380479	2	0	theme	adaptable	381:389	arg1	features					391:398	their environmentally friendly and adaptable features	346:398	their environmentally friendly and adaptable features	346:398	Polysaccharides, glycosidically linked monosaccharide units, are of particular interest because they serve as a promising material, owing to their environmentally friendly and adaptable features.
32380479	5	1	theme	carbonized	754:763	arg1	materials					765:773	carbonized materials	754:773	carbonized materials	754:773	Three kinds of metal-insulator-metal devices were fabricated using carbonized materials, and their carrier-transport properties were analyzed.
32380479	1	2	theme	high	172:175	arg1	performance					177:187	high performance	172:187	high performance	172:187	Attempts have been made to bring eco-friendly biomaterials into high-end electronic devices that require both high performance and durability.
32380479	4	3	theme	material	677:684	arg1	structure					660:668	the crystal structure	648:668	the crystal structure of the material	648:684	Chitosan composites incorporating nanoparticles were prepared and then carbonized to control the crystal structure of the material.
32380479	6	4	from	transport	979:987	arg1	material					1007:1014	the carbonized material	992:1014	the carbonized material	992:1014	The results showed that the addition of cellulose nano-whiskers (CNWs) into chitosan leads to a more ordered carbon structure, increasing the charge transport in the carbonized material.
32380479	6	5	theme	carbonized	996:1005	arg1	material					1007:1014	the carbonized material	992:1014	the carbonized material	992:1014	The results showed that the addition of cellulose nano-whiskers (CNWs) into chitosan leads to a more ordered carbon structure, increasing the charge transport in the carbonized material.
32380479	7	6	theme	green	1071:1075	arg1	composites					1077:1086	nanoparticle dispersed green composites	1048:1086	nanoparticle dispersed green composites	1048:1086	We anticipate that carbonizing nanoparticle dispersed green composites provides a new pathway for the development of sustainable and environmentally benign material systems.
32380479	5	7	theme	metal-insulator-metal	702:722	arg1	devices					724:730	metal-insulator-metal devices	702:730	metal-insulator-metal devices	702:730	Three kinds of metal-insulator-metal devices were fabricated using carbonized materials, and their carrier-transport properties were analyzed.
32380479	4	8	theme	Chitosan	555:562	arg1	composites					564:573	Chitosan composites	555:573	Chitosan composites incorporating nanoparticles	555:601	Chitosan composites incorporating nanoparticles were prepared and then carbonized to control the crystal structure of the material.
32380479	5	9	theme	devices	724:730	arg1	kinds					693:697	Three kinds	687:697	Three kinds of metal-insulator-metal devices	687:730	Three kinds of metal-insulator-metal devices were fabricated using carbonized materials, and their carrier-transport properties were analyzed.
32380479	7	10	theme	nanoparticle	1048:1059	arg1	composites					1077:1086	nanoparticle dispersed green composites	1048:1086	nanoparticle dispersed green composites	1048:1086	We anticipate that carbonizing nanoparticle dispersed green composites provides a new pathway for the development of sustainable and environmentally benign material systems.
32380479	0	11	theme	Carrier	0:6	arg1	transport					8:16	Carrier transport	0:16	Carrier transport of all	0:23	Carrier transport of all carbonized β-glucosic eco-materials.
32380479	7	12	theme	dispersed	1061:1069	arg1	composites					1077:1086	nanoparticle dispersed green composites	1048:1086	nanoparticle dispersed green composites	1048:1086	We anticipate that carbonizing nanoparticle dispersed green composites provides a new pathway for the development of sustainable and environmentally benign material systems.
32380479	3	13	theme	carbonized	411:420	arg1	eco-material					437:448	a carbonized polysaccharide eco-material	409:448	a carbonized polysaccharide eco-material encompassing nanoparticles and chitosan	409:488	We used a carbonized polysaccharide eco-material encompassing nanoparticles and chitosan to study the carrier-transport behavior of β-glucosic materials.
32380479	0	14	theme	all	21:23	arg1	transport					8:16	Carrier transport	0:16	Carrier transport of all	0:23	Carrier transport of all carbonized β-glucosic eco-materials.
32380479	3	15	used	used	404:407	arg2	We					401:402	We	401:402	We	401:402	We used a carbonized polysaccharide eco-material encompassing nanoparticles and chitosan to study the carrier-transport behavior of β-glucosic materials.
32380479	1	16	theme	eco-friendly	95:106	arg1	biomaterials					108:119	eco-friendly biomaterials	95:119	eco-friendly biomaterials	95:119	Attempts have been made to bring eco-friendly biomaterials into high-end electronic devices that require both high performance and durability.
32380479	4	17	theme	crystal	652:658	arg1	structure					660:668	the crystal structure	648:668	the crystal structure of the material	648:684	Chitosan composites incorporating nanoparticles were prepared and then carbonized to control the crystal structure of the material.
32380479	6	18	theme	charge	972:977	arg1	transport					979:987	the charge transport	968:987	the charge transport in the carbonized material	968:1014	The results showed that the addition of cellulose nano-whiskers (CNWs) into chitosan leads to a more ordered carbon structure, increasing the charge transport in the carbonized material.
32380479	2	19	theme	promising	317:325	arg1	material					327:334	a promising material	315:334	a promising material	315:334	Polysaccharides, glycosidically linked monosaccharide units, are of particular interest because they serve as a promising material, owing to their environmentally friendly and adaptable features.
32380479	2	19	theme	promising	317:325	arg1	they					301:304	they	301:304	they	301:304	Polysaccharides, glycosidically linked monosaccharide units, are of particular interest because they serve as a promising material, owing to their environmentally friendly and adaptable features.
32380479	0	20	theme	β-glucosic	36:45	arg1	eco-materials					47:59	β-glucosic eco-materials	36:59	β-glucosic eco-materials	36:59	Carrier transport of all carbonized β-glucosic eco-materials.
32380479	2	21	link	linked	237:242	arg1	units					259:263	glycosidically linked monosaccharide units	222:263	glycosidically linked monosaccharide units	222:263	Polysaccharides, glycosidically linked monosaccharide units, are of particular interest because they serve as a promising material, owing to their environmentally friendly and adaptable features.
32380479	2	21	link	linked	237:242	arg1	Polysaccharides					205:219	Polysaccharides	205:219	Polysaccharides	205:219	Polysaccharides, glycosidically linked monosaccharide units, are of particular interest because they serve as a promising material, owing to their environmentally friendly and adaptable features.
32380479	6	22	theme	nano-whiskers	880:892	arg1	addition					858:865	the addition	854:865	the addition of cellulose nano-whiskers (CNWs) into chitosan	854:913	The results showed that the addition of cellulose nano-whiskers (CNWs) into chitosan leads to a more ordered carbon structure, increasing the charge transport in the carbonized material.
32380479	3	23	theme	carrier-transport	503:519	arg1	behavior					521:528	the carrier-transport behavior	499:528	the carrier-transport behavior of β-glucosic materials	499:552	We used a carbonized polysaccharide eco-material encompassing nanoparticles and chitosan to study the carrier-transport behavior of β-glucosic materials.
32380479	3	24	theme	polysaccharide	422:435	arg1	eco-material					437:448	a carbonized polysaccharide eco-material	409:448	a carbonized polysaccharide eco-material encompassing nanoparticles and chitosan	409:488	We used a carbonized polysaccharide eco-material encompassing nanoparticles and chitosan to study the carrier-transport behavior of β-glucosic materials.
32380479	7	25	theme	material	1173:1180	arg1	systems					1182:1188	sustainable and environmentally benign material systems	1134:1188	sustainable and environmentally benign material systems	1134:1188	We anticipate that carbonizing nanoparticle dispersed green composites provides a new pathway for the development of sustainable and environmentally benign material systems.
32380479	6	26	theme	cellulose	870:878	arg1	CNWs					895:898	CNWs	895:898	CNWs	895:898	The results showed that the addition of cellulose nano-whiskers (CNWs) into chitosan leads to a more ordered carbon structure, increasing the charge transport in the carbonized material.
32380479	6	26	theme	cellulose	870:878	arg1	nano-whiskers					880:892	cellulose nano-whiskers	870:892	cellulose nano-whiskers (CNWs)	870:899	The results showed that the addition of cellulose nano-whiskers (CNWs) into chitosan leads to a more ordered carbon structure, increasing the charge transport in the carbonized material.
32380479	2	27	theme	friendly	368:375	arg1	features					391:398	their environmentally friendly and adaptable features	346:398	their environmentally friendly and adaptable features	346:398	Polysaccharides, glycosidically linked monosaccharide units, are of particular interest because they serve as a promising material, owing to their environmentally friendly and adaptable features.
32380479	7	28	theme	systems	1182:1188	arg1	development					1119:1129	the development	1115:1129	the development of sustainable and environmentally benign material systems	1115:1188	We anticipate that carbonizing nanoparticle dispersed green composites provides a new pathway for the development of sustainable and environmentally benign material systems.
32380479	2	29	theme	linked	237:242	arg1	units					259:263	glycosidically linked monosaccharide units	222:263	glycosidically linked monosaccharide units	222:263	Polysaccharides, glycosidically linked monosaccharide units, are of particular interest because they serve as a promising material, owing to their environmentally friendly and adaptable features.
32380479	2	29	theme	linked	237:242	arg1	Polysaccharides					205:219	Polysaccharides	205:219	Polysaccharides	205:219	Polysaccharides, glycosidically linked monosaccharide units, are of particular interest because they serve as a promising material, owing to their environmentally friendly and adaptable features.
32380479	2	30	theme	monosaccharide	244:257	arg1	units					259:263	glycosidically linked monosaccharide units	222:263	glycosidically linked monosaccharide units	222:263	Polysaccharides, glycosidically linked monosaccharide units, are of particular interest because they serve as a promising material, owing to their environmentally friendly and adaptable features.
32380479	2	30	theme	monosaccharide	244:257	arg1	Polysaccharides					205:219	Polysaccharides	205:219	Polysaccharides	205:219	Polysaccharides, glycosidically linked monosaccharide units, are of particular interest because they serve as a promising material, owing to their environmentally friendly and adaptable features.
32380479	1	31	theme	high-end	126:133	arg1	devices					146:152	high-end electronic devices	126:152	high-end electronic devices that require both high performance and durability	126:202	Attempts have been made to bring eco-friendly biomaterials into high-end electronic devices that require both high performance and durability.
32380479	5	32	theme	carrier-transport	786:802	arg1	properties					804:813	their carrier-transport properties	780:813	their carrier-transport properties	780:813	Three kinds of metal-insulator-metal devices were fabricated using carbonized materials, and their carrier-transport properties were analyzed.
32380479	6	33	theme	carbon	939:944	arg1	structure					946:954	a more ordered carbon structure	924:954	a more ordered carbon structure	924:954	The results showed that the addition of cellulose nano-whiskers (CNWs) into chitosan leads to a more ordered carbon structure, increasing the charge transport in the carbonized material.
32380479	1	34	theme	electronic	135:144	arg1	devices					146:152	high-end electronic devices	126:152	high-end electronic devices that require both high performance and durability	126:202	Attempts have been made to bring eco-friendly biomaterials into high-end electronic devices that require both high performance and durability.
32380479	7	35	theme	benign	1166:1171	arg1	systems					1182:1188	sustainable and environmentally benign material systems	1134:1188	sustainable and environmentally benign material systems	1134:1188	We anticipate that carbonizing nanoparticle dispersed green composites provides a new pathway for the development of sustainable and environmentally benign material systems.
32380479	6	36	theme	ordered	931:937	arg1	structure					946:954	a more ordered carbon structure	924:954	a more ordered carbon structure	924:954	The results showed that the addition of cellulose nano-whiskers (CNWs) into chitosan leads to a more ordered carbon structure, increasing the charge transport in the carbonized material.
32380479	7	37	theme	sustainable	1134:1144	arg1	systems					1182:1188	sustainable and environmentally benign material systems	1134:1188	sustainable and environmentally benign material systems	1134:1188	We anticipate that carbonizing nanoparticle dispersed green composites provides a new pathway for the development of sustainable and environmentally benign material systems.
32380479	3	38	theme	materials	544:552	arg1	behavior					521:528	the carrier-transport behavior	499:528	the carrier-transport behavior of β-glucosic materials	499:552	We used a carbonized polysaccharide eco-material encompassing nanoparticles and chitosan to study the carrier-transport behavior of β-glucosic materials.
32380479	3	39	theme	β-glucosic	533:542	arg1	materials					544:552	β-glucosic materials	533:552	β-glucosic materials	533:552	We used a carbonized polysaccharide eco-material encompassing nanoparticles and chitosan to study the carrier-transport behavior of β-glucosic materials.
32380479	2	40	theme	particular	273:282	arg1	interest					284:291	particular interest	273:291	particular interest	273:291	Polysaccharides, glycosidically linked monosaccharide units, are of particular interest because they serve as a promising material, owing to their environmentally friendly and adaptable features.
32380479	7	41	theme	new	1099:1101	arg1	pathway					1103:1109	a new pathway	1097:1109	a new pathway for the development of sustainable and environmentally benign material systems	1097:1188	We anticipate that carbonizing nanoparticle dispersed green composites provides a new pathway for the development of sustainable and environmentally benign material systems.
32846524	3	0	theme	P	679:679	arg1	levels					681:686	substance P levels	669:686	substance P levels	669:686	Results showed that DZMP at 200 mg/kg could significantly (P < 0.05) increase the intestinal transit rate, motilin, gastrin, substance P levels and concentration of short-chain fatty acids (SCFAs), reduce the somatostatin levels and improve the gastrointestinal peristalsis of rats.
32846524	2	1	from	effects	392:398	arg1	microbiota					422:431	intestinal microbiota	411:431	intestinal microbiota	411:431	In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride.
32846524	2	2	theme	zibethinus	341:350	arg1	DZMP					378:381	DZMP	378:381	DZMP	378:381	In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride.
32846524	2	2	theme	zibethinus	341:350	arg1	polysaccharide					362:375	Durio zibethinus Murr rind polysaccharide	335:375	Durio zibethinus Murr rind polysaccharide (DZMP)	335:382	In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride.
32846524	3	3	theme	gastrointestinal	789:804	arg1	peristalsis					806:816	the gastrointestinal peristalsis	785:816	the gastrointestinal peristalsis of rats	785:824	Results showed that DZMP at 200 mg/kg could significantly (P < 0.05) increase the intestinal transit rate, motilin, gastrin, substance P levels and concentration of short-chain fatty acids (SCFAs), reduce the somatostatin levels and improve the gastrointestinal peristalsis of rats.
32846524	2	4	theme	constipation	456:467	arg1	model					469:473	a constipation model	454:473	a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride	454:541	In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride.
32846524	6	5	contain	has	1281:1283	arg2	effect					1298:1303	a regulatory effect	1285:1303	a regulatory effect of treating functional constipation and regulating intestinal flora in rats	1285:1379	The results demonstrated that DZMP has a regulatory effect of treating functional constipation and regulating intestinal flora in rats.
32846524	6	5	contain	has	1281:1283	arg1	DZMP					1276:1279	DZMP	1276:1279	DZMP	1276:1279	The results demonstrated that DZMP has a regulatory effect of treating functional constipation and regulating intestinal flora in rats.
32846524	2	6	theme	Durio	335:339	arg1	DZMP					378:381	DZMP	378:381	DZMP	378:381	In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride.
32846524	2	6	theme	Durio	335:339	arg1	polysaccharide					362:375	Durio zibethinus Murr rind polysaccharide	335:375	Durio zibethinus Murr rind polysaccharide (DZMP)	335:382	In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride.
32846524	1	7	theme	life	248:251	arg1	quality					237:243	the quality	233:243	the quality of life in related patients	233:271	The prevalence of constipation increases rapidly with the increased pressure of some people's life, which seriously affects the quality of life in related patients.
32846524	2	8	theme	rats	498:501	arg1	model					469:473	a constipation model	454:473	a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride	454:541	In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride.
32846524	0	9	from	Effect	0:5	arg1	constipation					61:72	functional constipation	50:72	functional constipation	50:72	Effect of Durio zibethinus rind polysaccharide on functional constipation and intestinal microbiota in rats.
32846524	0	9	from	Effect	0:5	arg1	microbiota					89:98	intestinal microbiota	78:98	intestinal microbiota	78:98	Effect of Durio zibethinus rind polysaccharide on functional constipation and intestinal microbiota in rats.
32846524	3	10	theme	intestinal	626:635	arg1	rate					645:648	the intestinal transit rate	622:648	the intestinal transit rate	622:648	Results showed that DZMP at 200 mg/kg could significantly (P < 0.05) increase the intestinal transit rate, motilin, gastrin, substance P levels and concentration of short-chain fatty acids (SCFAs), reduce the somatostatin levels and improve the gastrointestinal peristalsis of rats.
32846524	5	11	theme	intestinal	1146:1155	arg1	composition					1168:1178	intestinal microbiota composition	1146:1178	intestinal microbiota composition	1146:1178	Principal co-ordinates analysis (PcoA) revealed a significant difference in intestinal microbiota composition between the model group and the high-dose DZMP group (200 mg/kg).
32846524	4	12	theme	model	1049:1053	arg1	group					1055:1059	the model group	1045:1059	the model group (4.19%)	1045:1067	Sequencing showed that the Lachnospiraceae-NK4A136-group in the rats given 200 mg/kg DZMP (16.07%) was significantly higher than that of the model group (10.13%), while the Desulfovibrio was lower (2.99%) than that of the model group (4.19%).
32846524	4	12	theme	model	1049:1053	arg1	%					1066:1066	4.19%	1062:1066	4.19%	1062:1066	Sequencing showed that the Lachnospiraceae-NK4A136-group in the rats given 200 mg/kg DZMP (16.07%) was significantly higher than that of the model group (10.13%), while the Desulfovibrio was lower (2.99%) than that of the model group (4.19%).
32846524	1	13	theme	related	256:262	arg1	patients					264:271	related patients	256:271	related patients	256:271	The prevalence of constipation increases rapidly with the increased pressure of some people's life, which seriously affects the quality of life in related patients.
32846524	4	14	theme	200 mg/kg	902:910	arg1	DZMP					912:915	200 mg/kg DZMP	902:915	200 mg/kg DZMP (16.07%)	902:924	Sequencing showed that the Lachnospiraceae-NK4A136-group in the rats given 200 mg/kg DZMP (16.07%) was significantly higher than that of the model group (10.13%), while the Desulfovibrio was lower (2.99%) than that of the model group (4.19%).
32846524	4	14	theme	200 mg/kg	902:910	arg1	%					923:923	16.07%	918:923	16.07%	918:923	Sequencing showed that the Lachnospiraceae-NK4A136-group in the rats given 200 mg/kg DZMP (16.07%) was significantly higher than that of the model group (10.13%), while the Desulfovibrio was lower (2.99%) than that of the model group (4.19%).
32846524	4	15	theme	model	968:972	arg1	group					974:978	the model group	964:978	the model group (10.13%)	964:987	Sequencing showed that the Lachnospiraceae-NK4A136-group in the rats given 200 mg/kg DZMP (16.07%) was significantly higher than that of the model group (10.13%), while the Desulfovibrio was lower (2.99%) than that of the model group (4.19%).
32846524	4	15	theme	model	968:972	arg1	%					986:986	10.13%	981:986	10.13%	981:986	Sequencing showed that the Lachnospiraceae-NK4A136-group in the rats given 200 mg/kg DZMP (16.07%) was significantly higher than that of the model group (10.13%), while the Desulfovibrio was lower (2.99%) than that of the model group (4.19%).
32846524	6	16	theme	intestinal	1356:1365	arg1	flora					1367:1371	intestinal flora	1356:1371	intestinal flora in rats	1356:1379	The results demonstrated that DZMP has a regulatory effect of treating functional constipation and regulating intestinal flora in rats.
32846524	0	17	theme	intestinal	78:87	arg1	microbiota					89:98	intestinal microbiota	78:98	intestinal microbiota	78:98	Effect of Durio zibethinus rind polysaccharide on functional constipation and intestinal microbiota in rats.
32846524	5	18	theme	Principal	1070:1078	arg1	PcoA					1103:1106	PcoA	1103:1106	PcoA	1103:1106	Principal co-ordinates analysis (PcoA) revealed a significant difference in intestinal microbiota composition between the model group and the high-dose DZMP group (200 mg/kg).
32846524	5	18	theme	Principal	1070:1078	arg1	analysis					1093:1100	Principal co-ordinates analysis	1070:1100	Principal co-ordinates analysis (PcoA)	1070:1107	Principal co-ordinates analysis (PcoA) revealed a significant difference in intestinal microbiota composition between the model group and the high-dose DZMP group (200 mg/kg).
32846524	2	19	theme	intestinal	411:420	arg1	microbiota					422:431	intestinal microbiota	411:431	intestinal microbiota	411:431	In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride.
32846524	3	20	theme	acids	727:731	arg1	motilin					651:657	motilin	651:657	motilin	651:657	Results showed that DZMP at 200 mg/kg could significantly (P < 0.05) increase the intestinal transit rate, motilin, gastrin, substance P levels and concentration of short-chain fatty acids (SCFAs), reduce the somatostatin levels and improve the gastrointestinal peristalsis of rats.
32846524	3	20	theme	acids	727:731	arg1	gastrin					660:666	gastrin	660:666	gastrin	660:666	Results showed that DZMP at 200 mg/kg could significantly (P < 0.05) increase the intestinal transit rate, motilin, gastrin, substance P levels and concentration of short-chain fatty acids (SCFAs), reduce the somatostatin levels and improve the gastrointestinal peristalsis of rats.
32846524	3	20	theme	acids	727:731	arg1	concentration					692:704	concentration	692:704	concentration	692:704	Results showed that DZMP at 200 mg/kg could significantly (P < 0.05) increase the intestinal transit rate, motilin, gastrin, substance P levels and concentration of short-chain fatty acids (SCFAs), reduce the somatostatin levels and improve the gastrointestinal peristalsis of rats.
32846524	3	20	theme	acids	727:731	arg1	levels					681:686	substance P levels	669:686	substance P levels	669:686	Results showed that DZMP at 200 mg/kg could significantly (P < 0.05) increase the intestinal transit rate, motilin, gastrin, substance P levels and concentration of short-chain fatty acids (SCFAs), reduce the somatostatin levels and improve the gastrointestinal peristalsis of rats.
32846524	3	20	theme	acids	727:731	arg1	rate					645:648	the intestinal transit rate	622:648	the intestinal transit rate	622:648	Results showed that DZMP at 200 mg/kg could significantly (P < 0.05) increase the intestinal transit rate, motilin, gastrin, substance P levels and concentration of short-chain fatty acids (SCFAs), reduce the somatostatin levels and improve the gastrointestinal peristalsis of rats.
32846524	5	21	theme	high-dose	1212:1220	arg1	group					1227:1231	the high-dose DZMP group	1208:1231	the high-dose DZMP group (200 mg/kg)	1208:1243	Principal co-ordinates analysis (PcoA) revealed a significant difference in intestinal microbiota composition between the model group and the high-dose DZMP group (200 mg/kg).
32846524	5	21	theme	high-dose	1212:1220	arg1	200 mg/kg					1234:1242	200 mg/kg	1234:1242	200 mg/kg	1234:1242	Principal co-ordinates analysis (PcoA) revealed a significant difference in intestinal microbiota composition between the model group and the high-dose DZMP group (200 mg/kg).
32846524	0	22	theme	zibethinus	16:25	arg1	polysaccharide					32:45	Durio zibethinus rind polysaccharide	10:45	Durio zibethinus rind polysaccharide	10:45	Effect of Durio zibethinus rind polysaccharide on functional constipation and intestinal microbiota in rats.
32846524	5	23	theme	co-ordinates	1080:1091	arg1	PcoA					1103:1106	PcoA	1103:1106	PcoA	1103:1106	Principal co-ordinates analysis (PcoA) revealed a significant difference in intestinal microbiota composition between the model group and the high-dose DZMP group (200 mg/kg).
32846524	5	23	theme	co-ordinates	1080:1091	arg1	analysis					1093:1100	Principal co-ordinates analysis	1070:1100	Principal co-ordinates analysis (PcoA)	1070:1107	Principal co-ordinates analysis (PcoA) revealed a significant difference in intestinal microbiota composition between the model group and the high-dose DZMP group (200 mg/kg).
32846524	4	24	dep	lower	1018:1022	arg1	%					1029:1029	2.99%	1025:1029	2.99%	1025:1029	Sequencing showed that the Lachnospiraceae-NK4A136-group in the rats given 200 mg/kg DZMP (16.07%) was significantly higher than that of the model group (10.13%), while the Desulfovibrio was lower (2.99%) than that of the model group (4.19%).
32846524	5	25	theme	DZMP	1222:1225	arg1	group					1227:1231	the high-dose DZMP group	1208:1231	the high-dose DZMP group (200 mg/kg)	1208:1243	Principal co-ordinates analysis (PcoA) revealed a significant difference in intestinal microbiota composition between the model group and the high-dose DZMP group (200 mg/kg).
32846524	5	25	theme	DZMP	1222:1225	arg1	200 mg/kg					1234:1242	200 mg/kg	1234:1242	200 mg/kg	1234:1242	Principal co-ordinates analysis (PcoA) revealed a significant difference in intestinal microbiota composition between the model group and the high-dose DZMP group (200 mg/kg).
32846524	3	26	dep	significantly	588:600	arg1	P < 0.05					603:610	P < 0.05	603:610	P < 0.05	603:610	Results showed that DZMP at 200 mg/kg could significantly (P < 0.05) increase the intestinal transit rate, motilin, gastrin, substance P levels and concentration of short-chain fatty acids (SCFAs), reduce the somatostatin levels and improve the gastrointestinal peristalsis of rats.
32846524	0	27	theme	Durio	10:14	arg1	polysaccharide					32:45	Durio zibethinus rind polysaccharide	10:45	Durio zibethinus rind polysaccharide	10:45	Effect of Durio zibethinus rind polysaccharide on functional constipation and intestinal microbiota in rats.
32846524	3	28	theme	short-chain	709:719	arg1	acids					727:731	short-chain fatty acids	709:731	short-chain fatty acids (SCFAs)	709:739	Results showed that DZMP at 200 mg/kg could significantly (P < 0.05) increase the intestinal transit rate, motilin, gastrin, substance P levels and concentration of short-chain fatty acids (SCFAs), reduce the somatostatin levels and improve the gastrointestinal peristalsis of rats.
32846524	3	28	theme	short-chain	709:719	arg1	SCFAs					734:738	SCFAs	734:738	SCFAs	734:738	Results showed that DZMP at 200 mg/kg could significantly (P < 0.05) increase the intestinal transit rate, motilin, gastrin, substance P levels and concentration of short-chain fatty acids (SCFAs), reduce the somatostatin levels and improve the gastrointestinal peristalsis of rats.
32846524	2	29	theme	loperamide	518:527	arg1	hydrochloride					529:541	loperamide hydrochloride	518:541	loperamide hydrochloride	518:541	In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride.
32846524	0	30	from	microbiota	89:98	arg1	rats					103:106	rats	103:106	rats	103:106	Effect of Durio zibethinus rind polysaccharide on functional constipation and intestinal microbiota in rats.
32846524	1	31	theme	increased	167:175	arg1	pressure					177:184	the increased pressure	163:184	the increased pressure of some people's life, which seriously affects the quality of life in related patients	163:271	The prevalence of constipation increases rapidly with the increased pressure of some people's life, which seriously affects the quality of life in related patients.
32846524	3	32	from	200 mg/kg	572:580	arg1	DZMP					564:567	DZMP	564:567	DZMP at 200 mg/kg	564:580	Results showed that DZMP at 200 mg/kg could significantly (P < 0.05) increase the intestinal transit rate, motilin, gastrin, substance P levels and concentration of short-chain fatty acids (SCFAs), reduce the somatostatin levels and improve the gastrointestinal peristalsis of rats.
32846524	1	33	theme	life	203:206	arg1	pressure					177:184	the increased pressure	163:184	the increased pressure of some people's life, which seriously affects the quality of life in related patients	163:271	The prevalence of constipation increases rapidly with the increased pressure of some people's life, which seriously affects the quality of life in related patients.
32846524	0	34	theme	polysaccharide	32:45	arg1	Effect					0:5	Effect	0:5	Effect of Durio zibethinus rind polysaccharide on functional constipation and intestinal microbiota in rats.	0:107	Effect of Durio zibethinus rind polysaccharide on functional constipation and intestinal microbiota in rats.
32846524	3	35	theme	fatty	721:725	arg1	acids					727:731	short-chain fatty acids	709:731	short-chain fatty acids (SCFAs)	709:739	Results showed that DZMP at 200 mg/kg could significantly (P < 0.05) increase the intestinal transit rate, motilin, gastrin, substance P levels and concentration of short-chain fatty acids (SCFAs), reduce the somatostatin levels and improve the gastrointestinal peristalsis of rats.
32846524	3	35	theme	fatty	721:725	arg1	SCFAs					734:738	SCFAs	734:738	SCFAs	734:738	Results showed that DZMP at 200 mg/kg could significantly (P < 0.05) increase the intestinal transit rate, motilin, gastrin, substance P levels and concentration of short-chain fatty acids (SCFAs), reduce the somatostatin levels and improve the gastrointestinal peristalsis of rats.
32846524	4	36	from	Lachnospiraceae-NK4A136-group	854:882	arg1	rats					891:894	the rats	887:894	the rats given 200 mg/kg DZMP (16.07%)	887:924	Sequencing showed that the Lachnospiraceae-NK4A136-group in the rats given 200 mg/kg DZMP (16.07%) was significantly higher than that of the model group (10.13%), while the Desulfovibrio was lower (2.99%) than that of the model group (4.19%).
32846524	3	37	theme	transit	637:643	arg1	rate					645:648	the intestinal transit rate	622:648	the intestinal transit rate	622:648	Results showed that DZMP at 200 mg/kg could significantly (P < 0.05) increase the intestinal transit rate, motilin, gastrin, substance P levels and concentration of short-chain fatty acids (SCFAs), reduce the somatostatin levels and improve the gastrointestinal peristalsis of rats.
32846524	0	38	theme	rind	27:30	arg1	polysaccharide					32:45	Durio zibethinus rind polysaccharide	10:45	Durio zibethinus rind polysaccharide	10:45	Effect of Durio zibethinus rind polysaccharide on functional constipation and intestinal microbiota in rats.
32846524	6	39	theme	functional	1317:1326	arg1	constipation					1328:1339	functional constipation	1317:1339	functional constipation	1317:1339	The results demonstrated that DZMP has a regulatory effect of treating functional constipation and regulating intestinal flora in rats.
32846524	6	40	from	flora	1367:1371	arg1	rats					1376:1379	rats	1376:1379	rats	1376:1379	The results demonstrated that DZMP has a regulatory effect of treating functional constipation and regulating intestinal flora in rats.
32846524	0	41	theme	functional	50:59	arg1	constipation					61:72	functional constipation	50:72	functional constipation	50:72	Effect of Durio zibethinus rind polysaccharide on functional constipation and intestinal microbiota in rats.
32846524	0	42	from	constipation	61:72	arg1	rats					103:106	rats	103:106	rats	103:106	Effect of Durio zibethinus rind polysaccharide on functional constipation and intestinal microbiota in rats.
32846524	2	43	from	polysaccharide	362:375	arg1	microbiota					422:431	intestinal microbiota	411:431	intestinal microbiota	411:431	In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride.
32846524	1	44	from	quality	237:243	arg1	patients					264:271	related patients	256:271	related patients	256:271	The prevalence of constipation increases rapidly with the increased pressure of some people's life, which seriously affects the quality of life in related patients.
32846524	2	45	theme	constipation	319:330	arg1	improvement					293:303	the improvement	289:303	the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota	289:431	In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride.
32846524	5	46	theme	model	1192:1196	arg1	group					1198:1202	the model group	1188:1202	the model group	1188:1202	Principal co-ordinates analysis (PcoA) revealed a significant difference in intestinal microbiota composition between the model group and the high-dose DZMP group (200 mg/kg).
32846524	1	47	theme	constipation	127:138	arg1	prevalence					113:122	The prevalence	109:122	The prevalence of constipation	109:138	The prevalence of constipation increases rapidly with the increased pressure of some people's life, which seriously affects the quality of life in related patients.
32846524	5	48	theme	significant	1120:1130	arg1	difference					1132:1141	a significant difference	1118:1141	a significant difference in intestinal microbiota composition between the model group and the high-dose DZMP group (200 mg/kg)	1118:1243	Principal co-ordinates analysis (PcoA) revealed a significant difference in intestinal microbiota composition between the model group and the high-dose DZMP group (200 mg/kg).
32846524	5	49	from	difference	1132:1141	arg1	composition					1168:1178	intestinal microbiota composition	1146:1178	intestinal microbiota composition	1146:1178	Principal co-ordinates analysis (PcoA) revealed a significant difference in intestinal microbiota composition between the model group and the high-dose DZMP group (200 mg/kg).
32846524	2	50	theme	functional	308:317	arg1	constipation					319:330	functional constipation	308:330	functional constipation	308:330	In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride.
32846524	2	51	theme	Sprague-Dawley	478:491	arg1	rats					498:501	Sprague-Dawley (SD) rats	478:501	Sprague-Dawley (SD) rats established by loperamide hydrochloride	478:541	In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride.
32846524	2	52	theme	rind	357:360	arg1	DZMP					378:381	DZMP	378:381	DZMP	378:381	In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride.
32846524	2	52	theme	rind	357:360	arg1	polysaccharide					362:375	Durio zibethinus Murr rind polysaccharide	335:375	Durio zibethinus Murr rind polysaccharide (DZMP)	335:382	In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride.
32846524	3	53	theme	rats	821:824	arg1	peristalsis					806:816	the gastrointestinal peristalsis	785:816	the gastrointestinal peristalsis of rats	785:824	Results showed that DZMP at 200 mg/kg could significantly (P < 0.05) increase the intestinal transit rate, motilin, gastrin, substance P levels and concentration of short-chain fatty acids (SCFAs), reduce the somatostatin levels and improve the gastrointestinal peristalsis of rats.
32846524	6	54	theme	regulatory	1287:1296	arg1	effect					1298:1303	a regulatory effect	1285:1303	a regulatory effect of treating functional constipation and regulating intestinal flora in rats	1285:1379	The results demonstrated that DZMP has a regulatory effect of treating functional constipation and regulating intestinal flora in rats.
32846524	3	55	theme	substance	669:677	arg1	levels					681:686	substance P levels	669:686	substance P levels	669:686	Results showed that DZMP at 200 mg/kg could significantly (P < 0.05) increase the intestinal transit rate, motilin, gastrin, substance P levels and concentration of short-chain fatty acids (SCFAs), reduce the somatostatin levels and improve the gastrointestinal peristalsis of rats.
32846524	2	56	theme	DZMP	403:406	arg1	DZMP					378:381	DZMP	378:381	DZMP	378:381	In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride.
32846524	2	56	theme	DZMP	403:406	arg1	polysaccharide					362:375	Durio zibethinus Murr rind polysaccharide	335:375	Durio zibethinus Murr rind polysaccharide (DZMP)	335:382	In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride.
32846524	2	56	theme	DZMP	403:406	arg1	effects					392:398	the effects	388:398	the effects of DZMP on intestinal microbiota	388:431	In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride.
32846524	2	57	theme	Murr	352:355	arg1	DZMP					378:381	DZMP	378:381	DZMP	378:381	In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride.
32846524	2	57	theme	Murr	352:355	arg1	polysaccharide					362:375	Durio zibethinus Murr rind polysaccharide	335:375	Durio zibethinus Murr rind polysaccharide (DZMP)	335:382	In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride.
32846524	5	58	theme	microbiota	1157:1166	arg1	composition					1168:1178	intestinal microbiota composition	1146:1178	intestinal microbiota composition	1146:1178	Principal co-ordinates analysis (PcoA) revealed a significant difference in intestinal microbiota composition between the model group and the high-dose DZMP group (200 mg/kg).
32846524	3	59	theme	somatostatin	753:764	arg1	levels					766:771	the somatostatin levels	749:771	the somatostatin levels	749:771	Results showed that DZMP at 200 mg/kg could significantly (P < 0.05) increase the intestinal transit rate, motilin, gastrin, substance P levels and concentration of short-chain fatty acids (SCFAs), reduce the somatostatin levels and improve the gastrointestinal peristalsis of rats.
32511389	5	0	theme	S	856:856	arg1	proteins					858:865	the S proteins	852:865	the S proteins	852:865	We then used CHARMM-GUI Membrane Builder to place the S proteins in a viral membrane and performed all-atom molecular dynamics simulations.
32511389	5	1	theme	dynamics	920:927	arg1	simulations					929:939	all-atom molecular dynamics simulations	901:939	all-atom molecular dynamics simulations	901:939	We then used CHARMM-GUI Membrane Builder to place the S proteins in a viral membrane and performed all-atom molecular dynamics simulations.
32511389	0	2	theme	Viral	80:84	arg1	Membrane					86:93	a Viral Membrane	78:93	a Viral Membrane	78:93	Developing a Fully-glycosylated Full-length SARS-CoV-2 Spike Protein Model in a Viral Membrane.
32511389	1	3	theme	fully-glycosylated	165:182	arg1	protein					217:223	a fully-glycosylated full-length SARS-CoV-2 spike (S) protein	163:223	a fully-glycosylated full-length SARS-CoV-2 spike (S) protein	163:223	This technical study describes all-atom modeling and simulation of a fully-glycosylated full-length SARS-CoV-2 spike (S) protein in a viral membrane.
32511389	4	4	theme	experimental	770:781	arg1	maps					783:786	their respective experimental maps	753:786	their respective experimental maps using ISOLDE	753:799	These fully-glycosylated full-length S protein model structures were assessed and further refined against the low-resolution data in their respective experimental maps using ISOLDE.
32511389	5	5	from	place	846:850	arg1	membrane					878:885	a viral membrane	870:885	a viral membrane	870:885	We then used CHARMM-GUI Membrane Builder to place the S proteins in a viral membrane and performed all-atom molecular dynamics simulations.
32511389	3	6	theme	monomer	554:560	arg1	N-glycans					521:529	22 N-glycans	518:529	22 N-glycans	518:529	Then, using the recently-determined most occupied glycoforms, 22 N-glycans and 1 O-glycan of each monomer were modeled using Glycan Reader & Modeler in CHARMM-GUI.
32511389	3	6	theme	monomer	554:560	arg1	O-glycan					537:544	1 O-glycan	535:544	1 O-glycan	535:544	Then, using the recently-determined most occupied glycoforms, 22 N-glycans and 1 O-glycan of each monomer were modeled using Glycan Reader & Modeler in CHARMM-GUI.
32511389	6	7	theme	simulation	1135:1144	arg1	research					1146:1153	simulation research	1135:1153	simulation research	1135:1153	All structures are available in CHARMM-GUI COVID-19 Archive (http://www.charmm-gui.org/docs/archive/covid19), so researchers can use these models to carry out innovative and novel modeling and simulation research for the prevention and treatment of COVID-19.
32511389	4	8	theme	protein	659:665	arg1	structures					673:682	These fully-glycosylated full-length S protein model structures	620:682	These fully-glycosylated full-length S protein model structures	620:682	These fully-glycosylated full-length S protein model structures were assessed and further refined against the low-resolution data in their respective experimental maps using ISOLDE.
32511389	3	9	theme	occupied	497:504	arg1	glycoforms					506:515	the recently-determined most occupied glycoforms	468:515	the recently-determined most occupied glycoforms	468:515	Then, using the recently-determined most occupied glycoforms, 22 N-glycans and 1 O-glycan of each monomer were modeled using Glycan Reader & Modeler in CHARMM-GUI.
32511389	4	10	gly	fully-glycosylated	626:643	arg1	structures					673:682	These fully-glycosylated full-length S protein model structures	620:682	These fully-glycosylated full-length S protein model structures	620:682	These fully-glycosylated full-length S protein model structures were assessed and further refined against the low-resolution data in their respective experimental maps using ISOLDE.
32511389	1	11	theme	full-length	184:194	arg1	protein					217:223	a fully-glycosylated full-length SARS-CoV-2 spike (S) protein	163:223	a fully-glycosylated full-length SARS-CoV-2 spike (S) protein	163:223	This technical study describes all-atom modeling and simulation of a fully-glycosylated full-length SARS-CoV-2 spike (S) protein in a viral membrane.
32511389	5	12	theme	Membrane	826:833	arg1	Builder					835:841	CHARMM-GUI Membrane Builder	815:841	CHARMM-GUI Membrane Builder	815:841	We then used CHARMM-GUI Membrane Builder to place the S proteins in a viral membrane and performed all-atom molecular dynamics simulations.
32511389	1	13	theme	SARS-CoV-2	196:205	arg1	protein					217:223	a fully-glycosylated full-length SARS-CoV-2 spike (S) protein	163:223	a fully-glycosylated full-length SARS-CoV-2 spike (S) protein	163:223	This technical study describes all-atom modeling and simulation of a fully-glycosylated full-length SARS-CoV-2 spike (S) protein in a viral membrane.
32511389	4	14	theme	respective	759:768	arg1	maps					783:786	their respective experimental maps	753:786	their respective experimental maps using ISOLDE	753:799	These fully-glycosylated full-length S protein model structures were assessed and further refined against the low-resolution data in their respective experimental maps using ISOLDE.
32511389	1	15	theme	spike	207:211	arg1	protein					217:223	a fully-glycosylated full-length SARS-CoV-2 spike (S) protein	163:223	a fully-glycosylated full-length SARS-CoV-2 spike (S) protein	163:223	This technical study describes all-atom modeling and simulation of a fully-glycosylated full-length SARS-CoV-2 spike (S) protein in a viral membrane.
32511389	5	16	theme	viral	872:876	arg1	membrane					878:885	a viral membrane	870:885	a viral membrane	870:885	We then used CHARMM-GUI Membrane Builder to place the S proteins in a viral membrane and performed all-atom molecular dynamics simulations.
32511389	2	17	theme	protein	300:306	arg1	structures					308:317	full-length S protein structures	286:317	full-length S protein structures	286:317	First, starting from PDB:6VSB and 6VXX, full-length S protein structures were modeled using template-based modeling, de-novo protein structure prediction, and loop modeling techniques in GALAXY modeling suite.
32511389	2	18	theme	structure	379:387	arg1	prediction					389:398	de-novo protein structure prediction	363:398	de-novo protein structure prediction	363:398	First, starting from PDB:6VSB and 6VXX, full-length S protein structures were modeled using template-based modeling, de-novo protein structure prediction, and loop modeling techniques in GALAXY modeling suite.
32511389	0	19	theme	Full-length	32:42	arg1	Model					69:73	a Fully-glycosylated Full-length SARS-CoV-2 Spike Protein Model	11:73	a Fully-glycosylated Full-length SARS-CoV-2 Spike Protein Model	11:73	Developing a Fully-glycosylated Full-length SARS-CoV-2 Spike Protein Model in a Viral Membrane.
32511389	6	20	theme	novel	1116:1120	arg1	modeling					1122:1129	novel modeling	1116:1129	novel modeling	1116:1129	All structures are available in CHARMM-GUI COVID-19 Archive (http://www.charmm-gui.org/docs/archive/covid19), so researchers can use these models to carry out innovative and novel modeling and simulation research for the prevention and treatment of COVID-19.
32511389	4	21	theme	low-resolution	730:743	arg1	data					745:748	the low-resolution data	726:748	the low-resolution data in their respective experimental maps using ISOLDE	726:799	These fully-glycosylated full-length S protein model structures were assessed and further refined against the low-resolution data in their respective experimental maps using ISOLDE.
32511389	1	22	theme	technical	101:109	arg1	study					111:115	This technical study	96:115	This technical study	96:115	This technical study describes all-atom modeling and simulation of a fully-glycosylated full-length SARS-CoV-2 spike (S) protein in a viral membrane.
32511389	1	23	theme	S	214:214	arg1	protein					217:223	a fully-glycosylated full-length SARS-CoV-2 spike (S) protein	163:223	a fully-glycosylated full-length SARS-CoV-2 spike (S) protein	163:223	This technical study describes all-atom modeling and simulation of a fully-glycosylated full-length SARS-CoV-2 spike (S) protein in a viral membrane.
32511389	0	24	theme	Fully-glycosylated	13:30	arg1	Model					69:73	a Fully-glycosylated Full-length SARS-CoV-2 Spike Protein Model	11:73	a Fully-glycosylated Full-length SARS-CoV-2 Spike Protein Model	11:73	Developing a Fully-glycosylated Full-length SARS-CoV-2 Spike Protein Model in a Viral Membrane.
32511389	6	25	from	Archive	994:1000	arg1	available					961:969	available	961:969	available	961:969	All structures are available in CHARMM-GUI COVID-19 Archive (http://www.charmm-gui.org/docs/archive/covid19), so researchers can use these models to carry out innovative and novel modeling and simulation research for the prevention and treatment of COVID-19.
32511389	2	26	theme	full-length	286:296	arg1	structures					308:317	full-length S protein structures	286:317	full-length S protein structures	286:317	First, starting from PDB:6VSB and 6VXX, full-length S protein structures were modeled using template-based modeling, de-novo protein structure prediction, and loop modeling techniques in GALAXY modeling suite.
32511389	4	27	theme	fully-glycosylated	626:643	arg1	structures					673:682	These fully-glycosylated full-length S protein model structures	620:682	These fully-glycosylated full-length S protein model structures	620:682	These fully-glycosylated full-length S protein model structures were assessed and further refined against the low-resolution data in their respective experimental maps using ISOLDE.
32511389	0	28	theme	Spike	55:59	arg1	Model					69:73	a Fully-glycosylated Full-length SARS-CoV-2 Spike Protein Model	11:73	a Fully-glycosylated Full-length SARS-CoV-2 Spike Protein Model	11:73	Developing a Fully-glycosylated Full-length SARS-CoV-2 Spike Protein Model in a Viral Membrane.
32511389	5	29	used	used	810:813	arg2	We					802:803	We	802:803	We	802:803	We then used CHARMM-GUI Membrane Builder to place the S proteins in a viral membrane and performed all-atom molecular dynamics simulations.
32511389	1	30	theme	protein	217:223	arg1	modeling					136:143	all-atom modeling and simulation	127:158	modeling	136:143	This technical study describes all-atom modeling and simulation of a fully-glycosylated full-length SARS-CoV-2 spike (S) protein in a viral membrane.
32511389	1	30	theme	protein	217:223	arg1	simulation					149:158	all-atom modeling and simulation	127:158	simulation	149:158	This technical study describes all-atom modeling and simulation of a fully-glycosylated full-length SARS-CoV-2 spike (S) protein in a viral membrane.
32511389	2	31	theme	modeling	410:417	arg1	techniques					419:428	loop modeling techniques	405:428	loop modeling techniques	405:428	First, starting from PDB:6VSB and 6VXX, full-length S protein structures were modeled using template-based modeling, de-novo protein structure prediction, and loop modeling techniques in GALAXY modeling suite.
32511389	0	32	theme	SARS-CoV-2	44:53	arg1	Model					69:73	a Fully-glycosylated Full-length SARS-CoV-2 Spike Protein Model	11:73	a Fully-glycosylated Full-length SARS-CoV-2 Spike Protein Model	11:73	Developing a Fully-glycosylated Full-length SARS-CoV-2 Spike Protein Model in a Viral Membrane.
32511389	6	33	theme	COVID-19	985:992	arg1	http					1003:1006	http://www.charmm-gui.org/docs/archive/covid19	1003:1048	http://www.charmm-gui.org/docs/archive/covid19	1003:1048	All structures are available in CHARMM-GUI COVID-19 Archive (http://www.charmm-gui.org/docs/archive/covid19), so researchers can use these models to carry out innovative and novel modeling and simulation research for the prevention and treatment of COVID-19.
32511389	6	33	theme	COVID-19	985:992	arg1	Archive					994:1000	CHARMM-GUI COVID-19 Archive	974:1000	CHARMM-GUI COVID-19 Archive (http://www.charmm-gui.org/docs/archive/covid19)	974:1049	All structures are available in CHARMM-GUI COVID-19 Archive (http://www.charmm-gui.org/docs/archive/covid19), so researchers can use these models to carry out innovative and novel modeling and simulation research for the prevention and treatment of COVID-19.
32511389	2	34	theme	modeling	440:447	arg1	suite					449:453	GALAXY modeling suite	433:453	GALAXY modeling suite	433:453	First, starting from PDB:6VSB and 6VXX, full-length S protein structures were modeled using template-based modeling, de-novo protein structure prediction, and loop modeling techniques in GALAXY modeling suite.
32511389	2	35	theme	loop	405:408	arg1	techniques					419:428	loop modeling techniques	405:428	loop modeling techniques	405:428	First, starting from PDB:6VSB and 6VXX, full-length S protein structures were modeled using template-based modeling, de-novo protein structure prediction, and loop modeling techniques in GALAXY modeling suite.
32511389	5	36	theme	CHARMM-GUI	815:824	arg1	Builder					835:841	CHARMM-GUI Membrane Builder	815:841	CHARMM-GUI Membrane Builder	815:841	We then used CHARMM-GUI Membrane Builder to place the S proteins in a viral membrane and performed all-atom molecular dynamics simulations.
32511389	5	37	theme	all-atom	901:908	arg1	simulations					929:939	all-atom molecular dynamics simulations	901:939	all-atom molecular dynamics simulations	901:939	We then used CHARMM-GUI Membrane Builder to place the S proteins in a viral membrane and performed all-atom molecular dynamics simulations.
32511389	0	38	gly	Fully-glycosylated	13:30	arg1	Model					69:73	a Fully-glycosylated Full-length SARS-CoV-2 Spike Protein Model	11:73	a Fully-glycosylated Full-length SARS-CoV-2 Spike Protein Model	11:73	Developing a Fully-glycosylated Full-length SARS-CoV-2 Spike Protein Model in a Viral Membrane.
32511389	2	39	theme	template-based	338:351	arg1	modeling					353:360	template-based modeling	338:360	template-based modeling	338:360	First, starting from PDB:6VSB and 6VXX, full-length S protein structures were modeled using template-based modeling, de-novo protein structure prediction, and loop modeling techniques in GALAXY modeling suite.
32511389	0	40	theme	Protein	61:67	arg1	Model					69:73	a Fully-glycosylated Full-length SARS-CoV-2 Spike Protein Model	11:73	a Fully-glycosylated Full-length SARS-CoV-2 Spike Protein Model	11:73	Developing a Fully-glycosylated Full-length SARS-CoV-2 Spike Protein Model in a Viral Membrane.
32511389	6	41	from	available	961:969	arg1	http					1003:1006	http://www.charmm-gui.org/docs/archive/covid19	1003:1048	http://www.charmm-gui.org/docs/archive/covid19	1003:1048	All structures are available in CHARMM-GUI COVID-19 Archive (http://www.charmm-gui.org/docs/archive/covid19), so researchers can use these models to carry out innovative and novel modeling and simulation research for the prevention and treatment of COVID-19.
32511389	6	41	from	available	961:969	arg1	Archive					994:1000	CHARMM-GUI COVID-19 Archive	974:1000	CHARMM-GUI COVID-19 Archive (http://www.charmm-gui.org/docs/archive/covid19)	974:1049	All structures are available in CHARMM-GUI COVID-19 Archive (http://www.charmm-gui.org/docs/archive/covid19), so researchers can use these models to carry out innovative and novel modeling and simulation research for the prevention and treatment of COVID-19.
32511389	6	42	theme	COVID-19	1191:1198	arg1	prevention					1163:1172	prevention	1163:1172	prevention	1163:1172	All structures are available in CHARMM-GUI COVID-19 Archive (http://www.charmm-gui.org/docs/archive/covid19), so researchers can use these models to carry out innovative and novel modeling and simulation research for the prevention and treatment of COVID-19.
32511389	6	42	theme	COVID-19	1191:1198	arg1	treatment					1178:1186	treatment	1178:1186	treatment	1178:1186	All structures are available in CHARMM-GUI COVID-19 Archive (http://www.charmm-gui.org/docs/archive/covid19), so researchers can use these models to carry out innovative and novel modeling and simulation research for the prevention and treatment of COVID-19.
32511389	1	43	theme	all-atom	127:134	arg1	modeling					136:143	all-atom modeling and simulation	127:158	modeling	136:143	This technical study describes all-atom modeling and simulation of a fully-glycosylated full-length SARS-CoV-2 spike (S) protein in a viral membrane.
32511389	1	44	gly	fully-glycosylated	165:182	arg1	protein					217:223	a fully-glycosylated full-length SARS-CoV-2 spike (S) protein	163:223	a fully-glycosylated full-length SARS-CoV-2 spike (S) protein	163:223	This technical study describes all-atom modeling and simulation of a fully-glycosylated full-length SARS-CoV-2 spike (S) protein in a viral membrane.
32511389	6	45	dep	prevention	1163:1172	arg1	the					1159:1161	the	1159:1161	the	1159:1161	All structures are available in CHARMM-GUI COVID-19 Archive (http://www.charmm-gui.org/docs/archive/covid19), so researchers can use these models to carry out innovative and novel modeling and simulation research for the prevention and treatment of COVID-19.
32511389	3	46	theme	recently-determined	472:490	arg1	glycoforms					506:515	the recently-determined most occupied glycoforms	468:515	the recently-determined most occupied glycoforms	468:515	Then, using the recently-determined most occupied glycoforms, 22 N-glycans and 1 O-glycan of each monomer were modeled using Glycan Reader & Modeler in CHARMM-GUI.
32511389	2	47	theme	S	298:298	arg1	structures					308:317	full-length S protein structures	286:317	full-length S protein structures	286:317	First, starting from PDB:6VSB and 6VXX, full-length S protein structures were modeled using template-based modeling, de-novo protein structure prediction, and loop modeling techniques in GALAXY modeling suite.
32511389	5	48	theme	molecular	910:918	arg1	simulations					929:939	all-atom molecular dynamics simulations	901:939	all-atom molecular dynamics simulations	901:939	We then used CHARMM-GUI Membrane Builder to place the S proteins in a viral membrane and performed all-atom molecular dynamics simulations.
32511389	1	49	theme	viral	230:234	arg1	membrane					236:243	a viral membrane	228:243	a viral membrane	228:243	This technical study describes all-atom modeling and simulation of a fully-glycosylated full-length SARS-CoV-2 spike (S) protein in a viral membrane.
32511389	4	50	theme	S	657:657	arg1	structures					673:682	These fully-glycosylated full-length S protein model structures	620:682	These fully-glycosylated full-length S protein model structures	620:682	These fully-glycosylated full-length S protein model structures were assessed and further refined against the low-resolution data in their respective experimental maps using ISOLDE.
32511389	4	51	theme	full-length	645:655	arg1	structures					673:682	These fully-glycosylated full-length S protein model structures	620:682	These fully-glycosylated full-length S protein model structures	620:682	These fully-glycosylated full-length S protein model structures were assessed and further refined against the low-resolution data in their respective experimental maps using ISOLDE.
32511389	2	52	theme	protein	371:377	arg1	prediction					389:398	de-novo protein structure prediction	363:398	de-novo protein structure prediction	363:398	First, starting from PDB:6VSB and 6VXX, full-length S protein structures were modeled using template-based modeling, de-novo protein structure prediction, and loop modeling techniques in GALAXY modeling suite.
32511389	4	53	theme	model	667:671	arg1	structures					673:682	These fully-glycosylated full-length S protein model structures	620:682	These fully-glycosylated full-length S protein model structures	620:682	These fully-glycosylated full-length S protein model structures were assessed and further refined against the low-resolution data in their respective experimental maps using ISOLDE.
32511389	4	54	from	data	745:748	arg1	maps					783:786	their respective experimental maps	753:786	their respective experimental maps using ISOLDE	753:799	These fully-glycosylated full-length S protein model structures were assessed and further refined against the low-resolution data in their respective experimental maps using ISOLDE.
32511389	2	55	theme	GALAXY	433:438	arg1	suite					449:453	GALAXY modeling suite	433:453	GALAXY modeling suite	433:453	First, starting from PDB:6VSB and 6VXX, full-length S protein structures were modeled using template-based modeling, de-novo protein structure prediction, and loop modeling techniques in GALAXY modeling suite.
32511389	2	56	theme	de-novo	363:369	arg1	prediction					389:398	de-novo protein structure prediction	363:398	de-novo protein structure prediction	363:398	First, starting from PDB:6VSB and 6VXX, full-length S protein structures were modeled using template-based modeling, de-novo protein structure prediction, and loop modeling techniques in GALAXY modeling suite.
32511389	6	57	theme	CHARMM-GUI	974:983	arg1	http					1003:1006	http://www.charmm-gui.org/docs/archive/covid19	1003:1048	http://www.charmm-gui.org/docs/archive/covid19	1003:1048	All structures are available in CHARMM-GUI COVID-19 Archive (http://www.charmm-gui.org/docs/archive/covid19), so researchers can use these models to carry out innovative and novel modeling and simulation research for the prevention and treatment of COVID-19.
32511389	6	57	theme	CHARMM-GUI	974:983	arg1	Archive					994:1000	CHARMM-GUI COVID-19 Archive	974:1000	CHARMM-GUI COVID-19 Archive (http://www.charmm-gui.org/docs/archive/covid19)	974:1049	All structures are available in CHARMM-GUI COVID-19 Archive (http://www.charmm-gui.org/docs/archive/covid19), so researchers can use these models to carry out innovative and novel modeling and simulation research for the prevention and treatment of COVID-19.
34579882	8	0	theme	composites	1587:1596	arg1	application					1562:1572	the application	1558:1572	the application of PVF-based composites	1558:1596	The bleeding time upon the application of PVF-based composites decreased by 78.3-90.4% (p < 0.001) compared to the application of well-known commercial product Celox™.
34579882	1	1	theme	death	214:218	arg1	cause					193:197	the leading cause	181:197	the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries	181:324	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	1	1	theme	death	214:218	arg1	loss					142:145	severe blood loss	129:145	severe blood loss due to hemorrhage	129:163	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	2	2	theme	hemostatic	517:526	arg1	materials					528:536	hemostatic materials	517:536	hemostatic materials	517:536	Herein we assessed the hemostatic properties of porous sponges based on biocompatible hydrophilic polymer, poly(vinyl formal) (PVF), which meets the main requirements for the development of hemostatic materials.
34579882	1	3	theme	patients	223:230	arg1	death					214:218	preventable death	202:218	preventable death of patients with military trauma	202:251	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	1	3	theme	patients	223:230	arg1	cause					276:280	the second leading cause	257:280	the second leading cause of death of civilian patients with injuries	257:324	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	4	4	theme	chitosan	783:790	arg1	hydrogel					792:799	chitosan hydrogel	783:799	chitosan hydrogel	783:799	Nano-sized aminopropyl silica, micro-sized calcium carbonate, and chitosan hydrogel were used to modify PVF matrixes.
34579882	7	5	theme	PVF-based	1378:1386	arg1	sponges					1395:1401	All investigated PVF-based porous sponges	1361:1401	All investigated PVF-based porous sponges	1361:1401	All investigated PVF-based porous sponges showed high hemostatic activity: upon the application of PVF-samples the bleeding decreased within 3 min by 68.4-94.4% (р < 0.001).
34579882	8	6	theme	commercial	1676:1685	arg1	Celox™					1695:1700	well-known commercial product Celox™	1665:1700	well-known commercial product Celox™	1665:1700	The bleeding time upon the application of PVF-based composites decreased by 78.3-90.4% (p < 0.001) compared to the application of well-known commercial product Celox™.
34579882	7	7	theme	hemostatic	1415:1424	arg1	activity					1426:1433	high hemostatic activity	1410:1433	high hemostatic activity	1410:1433	All investigated PVF-based porous sponges showed high hemostatic activity: upon the application of PVF-samples the bleeding decreased within 3 min by 68.4-94.4% (р < 0.001).
34579882	3	8	theme	vinyl	683:687	arg1	poly					678:681	poly	678:681	poly(vinyl alcohol) with formaldehyde	678:714	A series of composite hemostatic materials based on PVF sponges with different porosities and fillers were synthesized by acetalization of poly(vinyl alcohol) with formaldehyde.
34579882	3	8	theme	vinyl	683:687	arg1	alcohol					689:695	vinyl alcohol	683:695	vinyl alcohol	683:695	A series of composite hemostatic materials based on PVF sponges with different porosities and fillers were synthesized by acetalization of poly(vinyl alcohol) with formaldehyde.
34579882	8	9	theme	Celox™	1695:1700	arg1	application					1650:1660	the application	1646:1660	the application of well-known commercial product Celox™	1646:1700	The bleeding time upon the application of PVF-based composites decreased by 78.3-90.4% (p < 0.001) compared to the application of well-known commercial product Celox™.
34579882	2	10	theme	main	476:479	arg1	requirements					481:492	the main requirements	472:492	the main requirements for the development of hemostatic materials	472:536	Herein we assessed the hemostatic properties of porous sponges based on biocompatible hydrophilic polymer, poly(vinyl formal) (PVF), which meets the main requirements for the development of hemostatic materials.
34579882	3	11	theme	poly	678:681	arg1	acetalization					661:673	acetalization	661:673	acetalization of poly(vinyl alcohol) with formaldehyde	661:714	A series of composite hemostatic materials based on PVF sponges with different porosities and fillers were synthesized by acetalization of poly(vinyl alcohol) with formaldehyde.
34579882	6	12	theme	white	1304:1308	arg1	rat					1322:1324	white male Wistar rat	1304:1324	white male Wistar rat	1304:1324	Hemostatic properties of the materials were assessed using a model of parenchymal bleeding from the liver of white male Wistar rat with a gauze bandage as a control.
34579882	5	13	theme	nuclear	1167:1173	arg1	resonance					1184:1192	nuclear magnetic resonance	1167:1192	nuclear magnetic resonance	1167:1192	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	1	14	theme	military	237:244	arg1	trauma					246:251	military trauma	237:251	military trauma	237:251	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	6	15	theme	Hemostatic	1195:1204	arg1	properties					1206:1215	Hemostatic properties	1195:1215	Hemostatic properties of the materials	1195:1232	Hemostatic properties of the materials were assessed using a model of parenchymal bleeding from the liver of white male Wistar rat with a gauze bandage as a control.
34579882	5	16	theme	composite	981:989	arg1	sponges					991:997	the synthesized composite sponges	965:997	the synthesized composite sponges	965:997	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	4	17	theme	PVF	821:823	arg1	matrixes					825:832	PVF matrixes	821:832	PVF matrixes	821:832	Nano-sized aminopropyl silica, micro-sized calcium carbonate, and chitosan hydrogel were used to modify PVF matrixes.
34579882	3	18	theme	composite	551:559	arg1	materials					572:580	composite hemostatic materials	551:580	composite hemostatic materials based on PVF sponges with different porosities and fillers	551:639	A series of composite hemostatic materials based on PVF sponges with different porosities and fillers were synthesized by acetalization of poly(vinyl alcohol) with formaldehyde.
34579882	1	19	with	patients	303:310	arg1	injuries					317:324	injuries	317:324	injuries	317:324	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	5	20	theme	electron	1068:1075	arg1	microscopy					1077:1086	scanning electron microscopy	1059:1086	scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance	1059:1192	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	20	theme	electron	1068:1075	arg1	analysis					1029:1036	gravimetrical analysis	1015:1036	gravimetrical analysis	1015:1036	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	6	21	theme	gauze	1333:1337	arg1	bandage					1339:1345	a gauze bandage	1331:1345	a gauze bandage	1331:1345	Hemostatic properties of the materials were assessed using a model of parenchymal bleeding from the liver of white male Wistar rat with a gauze bandage as a control.
34579882	1	22	theme	severe	129:134	arg1	cause					193:197	the leading cause	181:197	the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries	181:324	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	1	22	theme	severe	129:134	arg1	loss					142:145	severe blood loss	129:145	severe blood loss due to hemorrhage	129:163	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	5	23	theme	optical	1039:1045	arg1	analysis					1029:1036	gravimetrical analysis	1015:1036	gravimetrical analysis	1015:1036	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	23	theme	optical	1039:1045	arg1	microscopy					1047:1056	optical microscopy	1039:1056	optical microscopy	1039:1056	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	24	dep	properties	855:864	arg1	degree					954:959	acetalization degree	940:959	acetalization degree	940:959	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	24	dep	properties	855:864	arg1	size					872:875	pore size	867:875	pore size	867:875	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	24	dep	properties	855:864	arg1	groups					912:917	functional groups	901:917	functional groups	901:917	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	24	dep	properties	855:864	arg1	hydrophilicity					920:933	hydrophilicity	920:933	hydrophilicity	920:933	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	24	dep	properties	855:864	arg1	composition					888:898	elemental composition	878:898	elemental composition	878:898	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	24	dep	properties	855:864	arg1	properties					855:864	The physicochemical properties	835:864	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges	835:997	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	6	25	theme	Wistar	1315:1320	arg1	rat					1322:1324	white male Wistar rat	1304:1324	white male Wistar rat	1304:1324	Hemostatic properties of the materials were assessed using a model of parenchymal bleeding from the liver of white male Wistar rat with a gauze bandage as a control.
34579882	3	26	with	poly	678:681	arg1	formaldehyde					703:714	formaldehyde	703:714	formaldehyde	703:714	A series of composite hemostatic materials based on PVF sponges with different porosities and fillers were synthesized by acetalization of poly(vinyl alcohol) with formaldehyde.
34579882	2	27	theme	biocompatible	399:411	arg1	polymer					425:431	biocompatible hydrophilic polymer	399:431	biocompatible hydrophilic polymer	399:431	Herein we assessed the hemostatic properties of porous sponges based on biocompatible hydrophilic polymer, poly(vinyl formal) (PVF), which meets the main requirements for the development of hemostatic materials.
34579882	2	27	theme	biocompatible	399:411	arg1	poly					434:437	poly	434:437	poly(vinyl formal) (PVF)	434:457	Herein we assessed the hemostatic properties of porous sponges based on biocompatible hydrophilic polymer, poly(vinyl formal) (PVF), which meets the main requirements for the development of hemostatic materials.
34579882	0	28	theme	Hemostatic	0:9	arg1	dressings					11:19	Hemostatic dressings	0:19	Hemostatic dressings	0:19	Hemostatic dressings based on poly(vinyl formal) sponges.
34579882	5	29	theme	elemental	878:886	arg1	composition					888:898	elemental composition	878:898	elemental composition	878:898	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	29	theme	elemental	878:886	arg1	properties					855:864	The physicochemical properties	835:864	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges	835:997	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	2	30	theme	porous	375:380	arg1	sponges					382:388	porous sponges	375:388	porous sponges	375:388	Herein we assessed the hemostatic properties of porous sponges based on biocompatible hydrophilic polymer, poly(vinyl formal) (PVF), which meets the main requirements for the development of hemostatic materials.
34579882	4	31	used	used	806:809	arg2	carbonate					768:776	micro-sized calcium carbonate	748:776	micro-sized calcium carbonate	748:776	Nano-sized aminopropyl silica, micro-sized calcium carbonate, and chitosan hydrogel were used to modify PVF matrixes.
34579882	4	31	used	used	806:809	arg2	hydrogel					792:799	chitosan hydrogel	783:799	chitosan hydrogel	783:799	Nano-sized aminopropyl silica, micro-sized calcium carbonate, and chitosan hydrogel were used to modify PVF matrixes.
34579882	4	31	used	used	806:809	arg2	silica					740:745	Nano-sized aminopropyl silica	717:745	Nano-sized aminopropyl silica	717:745	Nano-sized aminopropyl silica, micro-sized calcium carbonate, and chitosan hydrogel were used to modify PVF matrixes.
34579882	1	32	theme	hemostatic	83:92	arg1	agents					94:99	novel hemostatic agents	77:99	novel hemostatic agents	77:99	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	6	33	with	liver	1295:1299	arg1	bandage					1339:1345	a gauze bandage	1331:1345	a gauze bandage	1331:1345	Hemostatic properties of the materials were assessed using a model of parenchymal bleeding from the liver of white male Wistar rat with a gauze bandage as a control.
34579882	4	34	theme	calcium	760:766	arg1	carbonate					768:776	micro-sized calcium carbonate	748:776	micro-sized calcium carbonate	748:776	Nano-sized aminopropyl silica, micro-sized calcium carbonate, and chitosan hydrogel were used to modify PVF matrixes.
34579882	0	35	theme	poly	30:33	arg1	sponges					49:55	poly(vinyl formal) sponges	30:55	poly(vinyl formal) sponges	30:55	Hemostatic dressings based on poly(vinyl formal) sponges.
34579882	5	36	theme	energy	1102:1107	arg1	spectroscopy					1126:1137	energy dispersive x-ray spectroscopy	1102:1137	energy dispersive x-ray spectroscopy	1102:1137	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	3	37	theme	materials	572:580	arg1	series					541:546	A series	539:546	A series of composite hemostatic materials based on PVF sponges with different porosities and fillers	539:639	A series of composite hemostatic materials based on PVF sponges with different porosities and fillers were synthesized by acetalization of poly(vinyl alcohol) with formaldehyde.
34579882	5	38	theme	x-ray	1120:1124	arg1	spectroscopy					1126:1137	energy dispersive x-ray spectroscopy	1102:1137	energy dispersive x-ray spectroscopy	1102:1137	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	39	theme	synthesized	969:979	arg1	sponges					991:997	the synthesized composite sponges	965:997	the synthesized composite sponges	965:997	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	8	40	theme	bleeding	1539:1546	arg1	time					1548:1551	The bleeding time	1535:1551	The bleeding time upon the application of PVF-based composites	1535:1596	The bleeding time upon the application of PVF-based composites decreased by 78.3-90.4% (p < 0.001) compared to the application of well-known commercial product Celox™.
34579882	3	41	theme	different	608:616	arg1	porosities					618:627	different porosities	608:627	different porosities	608:627	A series of composite hemostatic materials based on PVF sponges with different porosities and fillers were synthesized by acetalization of poly(vinyl alcohol) with formaldehyde.
34579882	4	42	theme	aminopropyl	728:738	arg1	silica					740:745	Nano-sized aminopropyl silica	717:745	Nano-sized aminopropyl silica	717:745	Nano-sized aminopropyl silica, micro-sized calcium carbonate, and chitosan hydrogel were used to modify PVF matrixes.
34579882	7	43	theme	PVF-samples	1460:1470	arg1	application					1445:1455	the application	1441:1455	the application of PVF-samples	1441:1470	All investigated PVF-based porous sponges showed high hemostatic activity: upon the application of PVF-samples the bleeding decreased within 3 min by 68.4-94.4% (р < 0.001).
34579882	6	44	from	liver	1295:1299	arg1	control					1352:1358	a control	1350:1358	a control	1350:1358	Hemostatic properties of the materials were assessed using a model of parenchymal bleeding from the liver of white male Wistar rat with a gauze bandage as a control.
34579882	6	44	from	liver	1295:1299	arg1	model					1256:1260	a model	1254:1260	a model of parenchymal bleeding from the liver of white male Wistar rat with a gauze bandage	1254:1345	Hemostatic properties of the materials were assessed using a model of parenchymal bleeding from the liver of white male Wistar rat with a gauze bandage as a control.
34579882	6	44	from	liver	1295:1299	arg1	bleeding					1277:1284	bleeding	1277:1284	bleeding	1277:1284	Hemostatic properties of the materials were assessed using a model of parenchymal bleeding from the liver of white male Wistar rat with a gauze bandage as a control.
34579882	5	45	theme	dispersive	1109:1118	arg1	spectroscopy					1126:1137	energy dispersive x-ray spectroscopy	1102:1137	energy dispersive x-ray spectroscopy	1102:1137	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	1	46	theme	preventable	202:212	arg1	death					214:218	preventable death	202:218	preventable death of patients with military trauma	202:251	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	8	47	dep	decreased	1598:1606	arg1	p < 0.001					1623:1631	p < 0.001	1623:1631	p < 0.001	1623:1631	The bleeding time upon the application of PVF-based composites decreased by 78.3-90.4% (p < 0.001) compared to the application of well-known commercial product Celox™.
34579882	2	48	theme	materials	528:536	arg1	development					502:512	the development	498:512	the development of hemostatic materials	498:536	Herein we assessed the hemostatic properties of porous sponges based on biocompatible hydrophilic polymer, poly(vinyl formal) (PVF), which meets the main requirements for the development of hemostatic materials.
34579882	8	49	theme	PVF-based	1577:1585	arg1	composites					1587:1596	PVF-based composites	1577:1596	PVF-based composites	1577:1596	The bleeding time upon the application of PVF-based composites decreased by 78.3-90.4% (p < 0.001) compared to the application of well-known commercial product Celox™.
34579882	5	50	theme	magnetic	1175:1182	arg1	resonance					1184:1192	nuclear magnetic resonance	1167:1192	nuclear magnetic resonance	1167:1192	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	3	51	with	sponges	595:601	arg1	fillers					633:639	fillers	633:639	fillers	633:639	A series of composite hemostatic materials based on PVF sponges with different porosities and fillers were synthesized by acetalization of poly(vinyl alcohol) with formaldehyde.
34579882	3	51	with	sponges	595:601	arg1	porosities					618:627	different porosities	608:627	different porosities	608:627	A series of composite hemostatic materials based on PVF sponges with different porosities and fillers were synthesized by acetalization of poly(vinyl alcohol) with formaldehyde.
34579882	1	52	theme	blood	136:140	arg1	cause					193:197	the leading cause	181:197	the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries	181:324	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	1	52	theme	blood	136:140	arg1	loss					142:145	severe blood loss	129:145	severe blood loss due to hemorrhage	129:163	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	7	53	theme	high	1410:1413	arg1	activity					1426:1433	high hemostatic activity	1410:1433	high hemostatic activity	1410:1433	All investigated PVF-based porous sponges showed high hemostatic activity: upon the application of PVF-samples the bleeding decreased within 3 min by 68.4-94.4% (р < 0.001).
34579882	7	54	theme	porous	1388:1393	arg1	sponges					1395:1401	All investigated PVF-based porous sponges	1361:1401	All investigated PVF-based porous sponges	1361:1401	All investigated PVF-based porous sponges showed high hemostatic activity: upon the application of PVF-samples the bleeding decreased within 3 min by 68.4-94.4% (р < 0.001).
34579882	1	55	theme	leading	268:274	arg1	cause					276:280	the second leading cause	257:280	the second leading cause of death of civilian patients with injuries	257:324	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	6	56	theme	materials	1224:1232	arg1	properties					1206:1215	Hemostatic properties	1195:1215	Hemostatic properties of the materials	1195:1232	Hemostatic properties of the materials were assessed using a model of parenchymal bleeding from the liver of white male Wistar rat with a gauze bandage as a control.
34579882	8	57	theme	well-known	1665:1674	arg1	Celox™					1695:1700	well-known commercial product Celox™	1665:1700	well-known commercial product Celox™	1665:1700	The bleeding time upon the application of PVF-based composites decreased by 78.3-90.4% (p < 0.001) compared to the application of well-known commercial product Celox™.
34579882	7	58	theme	investigated	1365:1376	arg1	sponges					1395:1401	All investigated PVF-based porous sponges	1361:1401	All investigated PVF-based porous sponges	1361:1401	All investigated PVF-based porous sponges showed high hemostatic activity: upon the application of PVF-samples the bleeding decreased within 3 min by 68.4-94.4% (р < 0.001).
34579882	6	59	theme	male	1310:1313	arg1	rat					1322:1324	white male Wistar rat	1304:1324	white male Wistar rat	1304:1324	Hemostatic properties of the materials were assessed using a model of parenchymal bleeding from the liver of white male Wistar rat with a gauze bandage as a control.
34579882	0	60	dep	poly	30:33	arg1	formal					41:46	formal	41:46	formal	41:46	Hemostatic dressings based on poly(vinyl formal) sponges.
34579882	8	61	theme	product	1687:1693	arg1	Celox™					1695:1700	well-known commercial product Celox™	1665:1700	well-known commercial product Celox™	1665:1700	The bleeding time upon the application of PVF-based composites decreased by 78.3-90.4% (p < 0.001) compared to the application of well-known commercial product Celox™.
34579882	5	62	theme	physicochemical	839:853	arg1	composition					888:898	elemental composition	878:898	elemental composition	878:898	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	62	theme	physicochemical	839:853	arg1	degree					954:959	acetalization degree	940:959	acetalization degree	940:959	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	62	theme	physicochemical	839:853	arg1	hydrophilicity					920:933	hydrophilicity	920:933	hydrophilicity	920:933	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	62	theme	physicochemical	839:853	arg1	properties					855:864	The physicochemical properties	835:864	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges	835:997	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	62	theme	physicochemical	839:853	arg1	size					872:875	pore size	867:875	pore size	867:875	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	63	theme	gravimetrical	1015:1027	arg1	microscopy					1077:1086	scanning electron microscopy	1059:1086	scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance	1059:1192	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	63	theme	gravimetrical	1015:1027	arg1	analysis					1029:1036	gravimetrical analysis	1015:1036	gravimetrical analysis	1015:1036	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	63	theme	gravimetrical	1015:1027	arg1	microscopy					1047:1056	optical microscopy	1039:1056	optical microscopy	1039:1056	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	1	64	theme	agents	94:99	arg1	related					104:110	related	104:110	related	104:110	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	1	64	theme	agents	94:99	arg1	development					62:72	The development	58:72	The development of novel hemostatic agents	58:99	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	6	65	theme	bleeding	1277:1284	arg1	control					1352:1358	a control	1350:1358	a control	1350:1358	Hemostatic properties of the materials were assessed using a model of parenchymal bleeding from the liver of white male Wistar rat with a gauze bandage as a control.
34579882	6	65	theme	bleeding	1277:1284	arg1	model					1256:1260	a model	1254:1260	a model of parenchymal bleeding from the liver of white male Wistar rat with a gauze bandage	1254:1345	Hemostatic properties of the materials were assessed using a model of parenchymal bleeding from the liver of white male Wistar rat with a gauze bandage as a control.
34579882	5	66	theme	sponges	991:997	arg1	composition					888:898	elemental composition	878:898	elemental composition	878:898	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	66	theme	sponges	991:997	arg1	degree					954:959	acetalization degree	940:959	acetalization degree	940:959	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	66	theme	sponges	991:997	arg1	hydrophilicity					920:933	hydrophilicity	920:933	hydrophilicity	920:933	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	66	theme	sponges	991:997	arg1	properties					855:864	The physicochemical properties	835:864	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges	835:997	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	66	theme	sponges	991:997	arg1	size					872:875	pore size	867:875	pore size	867:875	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	67	theme	pore	867:870	arg1	properties					855:864	The physicochemical properties	835:864	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges	835:997	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	67	theme	pore	867:870	arg1	size					872:875	pore size	867:875	pore size	867:875	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	1	68	theme	death	285:289	arg1	death					214:218	preventable death	202:218	preventable death of patients with military trauma	202:251	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	1	68	theme	death	285:289	arg1	cause					276:280	the second leading cause	257:280	the second leading cause of death of civilian patients with injuries	257:324	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	1	69	theme	patients	303:310	arg1	death					285:289	death	285:289	death of civilian patients with injuries	285:324	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	1	70	theme	civilian	294:301	arg1	patients					303:310	civilian patients	294:310	civilian patients with injuries	294:324	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	2	71	dep	poly	434:437	arg1	formal					445:450	formal	445:450	formal	445:450	Herein we assessed the hemostatic properties of porous sponges based on biocompatible hydrophilic polymer, poly(vinyl formal) (PVF), which meets the main requirements for the development of hemostatic materials.
34579882	5	72	theme	functional	901:910	arg1	groups					912:917	functional groups	901:917	functional groups	901:917	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	6	73	theme	rat	1322:1324	arg1	liver					1295:1299	the liver	1291:1299	the liver of white male Wistar rat with a gauze bandage	1291:1345	Hemostatic properties of the materials were assessed using a model of parenchymal bleeding from the liver of white male Wistar rat with a gauze bandage as a control.
34579882	5	74	theme	scanning	1059:1066	arg1	microscopy					1077:1086	scanning electron microscopy	1059:1086	scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance	1059:1192	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	74	theme	scanning	1059:1066	arg1	analysis					1029:1036	gravimetrical analysis	1015:1036	gravimetrical analysis	1015:1036	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	75	theme	infrared	1140:1147	arg1	spectroscopy					1149:1160	infrared spectroscopy	1140:1160	infrared spectroscopy	1140:1160	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	1	76	theme	novel	77:81	arg1	agents					94:99	novel hemostatic agents	77:99	novel hemostatic agents	77:99	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	2	77	theme	sponges	382:388	arg1	properties					361:370	the hemostatic properties	346:370	the hemostatic properties of porous sponges based on biocompatible hydrophilic polymer, poly(vinyl formal) (PVF), which meets the main requirements for the development of hemostatic materials	346:536	Herein we assessed the hemostatic properties of porous sponges based on biocompatible hydrophilic polymer, poly(vinyl formal) (PVF), which meets the main requirements for the development of hemostatic materials.
34579882	3	78	theme	hemostatic	561:570	arg1	materials					572:580	composite hemostatic materials	551:580	composite hemostatic materials based on PVF sponges with different porosities and fillers	551:639	A series of composite hemostatic materials based on PVF sponges with different porosities and fillers were synthesized by acetalization of poly(vinyl alcohol) with formaldehyde.
34579882	4	79	theme	micro-sized	748:758	arg1	carbonate					768:776	micro-sized calcium carbonate	748:776	micro-sized calcium carbonate	748:776	Nano-sized aminopropyl silica, micro-sized calcium carbonate, and chitosan hydrogel were used to modify PVF matrixes.
34579882	2	80	theme	hemostatic	350:359	arg1	properties					361:370	the hemostatic properties	346:370	the hemostatic properties of porous sponges based on biocompatible hydrophilic polymer, poly(vinyl formal) (PVF), which meets the main requirements for the development of hemostatic materials	346:536	Herein we assessed the hemostatic properties of porous sponges based on biocompatible hydrophilic polymer, poly(vinyl formal) (PVF), which meets the main requirements for the development of hemostatic materials.
34579882	1	81	theme	second	261:266	arg1	cause					276:280	the second leading cause	257:280	the second leading cause of death of civilian patients with injuries	257:324	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	1	82	with	patients	223:230	arg1	trauma					246:251	military trauma	237:251	military trauma	237:251	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	2	83	theme	hydrophilic	413:423	arg1	polymer					425:431	biocompatible hydrophilic polymer	399:431	biocompatible hydrophilic polymer	399:431	Herein we assessed the hemostatic properties of porous sponges based on biocompatible hydrophilic polymer, poly(vinyl formal) (PVF), which meets the main requirements for the development of hemostatic materials.
34579882	2	83	theme	hydrophilic	413:423	arg1	poly					434:437	poly	434:437	poly(vinyl formal) (PVF)	434:457	Herein we assessed the hemostatic properties of porous sponges based on biocompatible hydrophilic polymer, poly(vinyl formal) (PVF), which meets the main requirements for the development of hemostatic materials.
34579882	1	84	theme	cause	276:280	arg1	cause					193:197	the leading cause	181:197	the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries	181:324	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	1	84	theme	cause	276:280	arg1	loss					142:145	severe blood loss	129:145	severe blood loss due to hemorrhage	129:163	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	3	85	theme	PVF	591:593	arg1	sponges					595:601	PVF sponges	591:601	PVF sponges with different porosities and fillers	591:639	A series of composite hemostatic materials based on PVF sponges with different porosities and fillers were synthesized by acetalization of poly(vinyl alcohol) with formaldehyde.
34579882	4	86	theme	Nano-sized	717:726	arg1	silica					740:745	Nano-sized aminopropyl silica	717:745	Nano-sized aminopropyl silica	717:745	Nano-sized aminopropyl silica, micro-sized calcium carbonate, and chitosan hydrogel were used to modify PVF matrixes.
34579882	1	87	theme	leading	185:191	arg1	cause					193:197	the leading cause	181:197	the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries	181:324	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	1	87	theme	leading	185:191	arg1	loss					142:145	severe blood loss	129:145	severe blood loss due to hemorrhage	129:163	The development of novel hemostatic agents is related to the fact that severe blood loss due to hemorrhage continues to be the leading cause of preventable death of patients with military trauma and the second leading cause of death of civilian patients with injuries.
34579882	5	88	theme	acetalization	940:952	arg1	degree					954:959	acetalization degree	940:959	acetalization degree	940:959	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	5	88	theme	acetalization	940:952	arg1	properties					855:864	The physicochemical properties	835:864	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges	835:997	The physicochemical properties (pore size, elemental composition, functional groups, hydrophilicity, and acetalization degree) of the synthesized composite sponges were studied by gravimetrical analysis, optical microscopy, scanning electron microscopy combined with energy dispersive x-ray spectroscopy, infrared spectroscopy, and nuclear magnetic resonance.
34579882	6	89	from	model	1256:1260	arg1	liver					1295:1299	the liver	1291:1299	the liver of white male Wistar rat with a gauze bandage	1291:1345	Hemostatic properties of the materials were assessed using a model of parenchymal bleeding from the liver of white male Wistar rat with a gauze bandage as a control.
33357691	8	0	from	improvement	1846:1856	arg1	performance					1872:1882	the growth performance	1861:1882	the growth performance of immunosuppressive broilers	1861:1912	The results indicated that IAPS are effective in increasing the cecal beneficial bacteria and short-chain fatty acids production, contributing to improvement in the growth performance of immunosuppressive broilers.
33357691	8	1	theme	cecal	1764:1768	arg1	bacteria					1781:1788	the cecal beneficial bacteria	1760:1788	the cecal beneficial bacteria	1760:1788	The results indicated that IAPS are effective in increasing the cecal beneficial bacteria and short-chain fatty acids production, contributing to improvement in the growth performance of immunosuppressive broilers.
33357691	6	2	theme	Ruminococcaceae	1434:1448	arg1	proportions					1419:1429	higher proportions	1412:1429	higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05)	1412:1555	Specifically, broilers fed diets containing IAPS showed lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus and higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05).
33357691	6	2	theme	Ruminococcaceae	1434:1448	arg1	abundances					1344:1353	lower abundances	1338:1353	lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus	1338:1406	Specifically, broilers fed diets containing IAPS showed lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus and higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05).
33357691	1	3	theme	microbiota	295:304	arg1	populations					306:316	cecal microbiota populations	289:316	cecal microbiota populations	289:316	This study was designed to evaluate the effects of γ-irradiated Astragalus polysaccharides (IAPS) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers.
33357691	7	4	theme	cecal	1685:1689	arg1	digesta					1691:1697	cecal digesta	1685:1697	cecal digesta	1685:1697	The IAPS treatment also increased butyrate concentration (P < 0.05) and tended to elevate acetate concentration (P = 0.052) in cecal digesta.
33357691	8	5	theme	bacteria	1781:1788	arg1	production					1818:1827	the cecal beneficial bacteria and short-chain fatty acids production	1760:1827	the cecal beneficial bacteria and short-chain fatty acids production	1760:1827	The results indicated that IAPS are effective in increasing the cecal beneficial bacteria and short-chain fatty acids production, contributing to improvement in the growth performance of immunosuppressive broilers.
33357691	4	6	theme	microbial	1031:1039	arg1	diversity					1041:1049	the overall microbial diversity	1019:1049	the overall microbial diversity	1019:1049	The trial lasted 21 d. Compared with the control group, CPM treatment decreased the broiler average daily gain (ADG) and feed intake (P < 0.05) but did not affect the overall microbial diversity and compositions, as well as the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05).
33357691	8	7	theme	short-chain	1794:1804	arg1	acids					1812:1816	short-chain fatty acids	1794:1816	short-chain fatty acids	1794:1816	The results indicated that IAPS are effective in increasing the cecal beneficial bacteria and short-chain fatty acids production, contributing to improvement in the growth performance of immunosuppressive broilers.
33357691	6	8	theme	Mollicutes	1509:1518	arg1	proportions					1419:1429	higher proportions	1412:1429	higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05)	1412:1555	Specifically, broilers fed diets containing IAPS showed lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus and higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05).
33357691	6	8	theme	Mollicutes	1509:1518	arg1	abundances					1344:1353	lower abundances	1338:1353	lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus	1338:1406	Specifically, broilers fed diets containing IAPS showed lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus and higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05).
33357691	3	9	theme	sterilized	720:729	arg1	saline					731:736	0.5 mL sterilized saline	713:736	0.5 mL sterilized saline (0.75%, wt/vol)	713:752	On day 16, 18, and 20, broilers in the control group were intramuscularly injected with 0.5 mL sterilized saline (0.75%, wt/vol), and those in the CPM and IAPS groups were intramuscularly injected with 0.5 mL CPM (40 mg/kg of BW).
33357691	3	9	theme	sterilized	720:729	arg1	%					743:743	0.75%	739:743	0.75%	739:743	On day 16, 18, and 20, broilers in the control group were intramuscularly injected with 0.5 mL sterilized saline (0.75%, wt/vol), and those in the CPM and IAPS groups were intramuscularly injected with 0.5 mL CPM (40 mg/kg of BW).
33357691	0	10	theme	Astragalus	140:149	arg1	polysaccharides					151:165	γ-irradiated Astragalus polysaccharides	127:165	γ-irradiated Astragalus polysaccharides	127:165	Microbiota populations and short-chain fatty acids production in cecum of immunosuppressed broilers consuming diets containing γ-irradiated Astragalus polysaccharides.
33357691	1	11	theme	Astragalus	232:241	arg1	IAPS					260:263	IAPS	260:263	IAPS	260:263	This study was designed to evaluate the effects of γ-irradiated Astragalus polysaccharides (IAPS) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers.
33357691	1	11	theme	Astragalus	232:241	arg1	polysaccharides					243:257	γ-irradiated Astragalus polysaccharides	219:257	γ-irradiated Astragalus polysaccharides (IAPS)	219:264	This study was designed to evaluate the effects of γ-irradiated Astragalus polysaccharides (IAPS) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers.
33357691	1	12	from	effects	208:214	arg1	populations					306:316	cecal microbiota populations	289:316	cecal microbiota populations	289:316	This study was designed to evaluate the effects of γ-irradiated Astragalus polysaccharides (IAPS) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers.
33357691	1	12	from	effects	208:214	arg1	concentrations					323:336	concentrations	323:336	concentrations of cecal short-chain fatty acids of immunosuppressed broilers	323:398	This study was designed to evaluate the effects of γ-irradiated Astragalus polysaccharides (IAPS) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers.
33357691	1	12	from	effects	208:214	arg1	performance					276:286	growth performance	269:286	growth performance	269:286	This study was designed to evaluate the effects of γ-irradiated Astragalus polysaccharides (IAPS) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers.
33357691	8	13	theme	growth	1865:1870	arg1	performance					1872:1882	the growth performance	1861:1882	the growth performance of immunosuppressive broilers	1861:1912	The results indicated that IAPS are effective in increasing the cecal beneficial bacteria and short-chain fatty acids production, contributing to improvement in the growth performance of immunosuppressive broilers.
33357691	1	14	theme	acids	365:369	arg1	populations					306:316	cecal microbiota populations	289:316	cecal microbiota populations	289:316	This study was designed to evaluate the effects of γ-irradiated Astragalus polysaccharides (IAPS) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers.
33357691	1	14	theme	acids	365:369	arg1	concentrations					323:336	concentrations	323:336	concentrations of cecal short-chain fatty acids of immunosuppressed broilers	323:398	This study was designed to evaluate the effects of γ-irradiated Astragalus polysaccharides (IAPS) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers.
33357691	1	14	theme	acids	365:369	arg1	performance					276:286	growth performance	269:286	growth performance	269:286	This study was designed to evaluate the effects of γ-irradiated Astragalus polysaccharides (IAPS) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers.
33357691	4	15	theme	CPM	912:914	arg1	treatment					916:924	CPM treatment	912:924	CPM treatment	912:924	The trial lasted 21 d. Compared with the control group, CPM treatment decreased the broiler average daily gain (ADG) and feed intake (P < 0.05) but did not affect the overall microbial diversity and compositions, as well as the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05).
33357691	0	16	theme	immunosuppressed	74:89	arg1	broilers					91:98	immunosuppressed broilers	74:98	immunosuppressed broilers consuming diets containing γ-irradiated Astragalus polysaccharides	74:165	Microbiota populations and short-chain fatty acids production in cecum of immunosuppressed broilers consuming diets containing γ-irradiated Astragalus polysaccharides.
33357691	1	17	theme	immunosuppressed	374:389	arg1	broilers					391:398	immunosuppressed broilers	374:398	immunosuppressed broilers	374:398	This study was designed to evaluate the effects of γ-irradiated Astragalus polysaccharides (IAPS) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers.
33357691	4	18	theme	propionate	1117:1126	arg1	concentrations					1084:1097	the concentrations	1080:1097	the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05)	1080:1172	The trial lasted 21 d. Compared with the control group, CPM treatment decreased the broiler average daily gain (ADG) and feed intake (P < 0.05) but did not affect the overall microbial diversity and compositions, as well as the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05).
33357691	8	19	theme	broilers	1905:1912	arg1	performance					1872:1882	the growth performance	1861:1882	the growth performance of immunosuppressive broilers	1861:1912	The results indicated that IAPS are effective in increasing the cecal beneficial bacteria and short-chain fatty acids production, contributing to improvement in the growth performance of immunosuppressive broilers.
33357691	3	20	dep	%	743:743	arg1	wt/vol					746:751	wt/vol	746:751	wt/vol	746:751	On day 16, 18, and 20, broilers in the control group were intramuscularly injected with 0.5 mL sterilized saline (0.75%, wt/vol), and those in the CPM and IAPS groups were intramuscularly injected with 0.5 mL CPM (40 mg/kg of BW).
33357691	7	21	theme	butyrate	1592:1599	arg1	P < 0.05					1616:1623	P < 0.05	1616:1623	P < 0.05	1616:1623	The IAPS treatment also increased butyrate concentration (P < 0.05) and tended to elevate acetate concentration (P = 0.052) in cecal digesta.
33357691	7	21	theme	butyrate	1592:1599	arg1	concentration					1601:1613	butyrate concentration	1592:1613	butyrate concentration (P < 0.05)	1592:1624	The IAPS treatment also increased butyrate concentration (P < 0.05) and tended to elevate acetate concentration (P = 0.052) in cecal digesta.
33357691	2	22	theme	-treated	531:538	arg1	groups					540:545	cyclophosphamide (CPM)-treated groups	509:545	cyclophosphamide (CPM)-treated groups fed either a basal diet or the diets containing 900 mg/kg IAPS, respectively	509:622	A total of 144 one-day-old broiler chicks were randomly assigned into 3 groups: nontreated group (control), cyclophosphamide (CPM)-treated groups fed either a basal diet or the diets containing 900 mg/kg IAPS, respectively.
33357691	2	22	theme	-treated	531:538	arg1	groups					473:478	3 groups	471:478	3 groups	471:478	A total of 144 one-day-old broiler chicks were randomly assigned into 3 groups: nontreated group (control), cyclophosphamide (CPM)-treated groups fed either a basal diet or the diets containing 900 mg/kg IAPS, respectively.
33357691	0	23	from	production	51:60	arg1	cecum					65:69	cecum	65:69	cecum of immunosuppressed broilers consuming diets containing γ-irradiated Astragalus polysaccharides	65:165	Microbiota populations and short-chain fatty acids production in cecum of immunosuppressed broilers consuming diets containing γ-irradiated Astragalus polysaccharides.
33357691	2	24	theme	chicks	436:441	arg1	total					403:407	A total	401:407	A total of 144 one-day-old broiler chicks	401:441	A total of 144 one-day-old broiler chicks were randomly assigned into 3 groups: nontreated group (control), cyclophosphamide (CPM)-treated groups fed either a basal diet or the diets containing 900 mg/kg IAPS, respectively.
33357691	2	25	theme	nontreated	481:490	arg1	control					499:505	control	499:505	control	499:505	A total of 144 one-day-old broiler chicks were randomly assigned into 3 groups: nontreated group (control), cyclophosphamide (CPM)-treated groups fed either a basal diet or the diets containing 900 mg/kg IAPS, respectively.
33357691	2	25	theme	nontreated	481:490	arg1	group					492:496	nontreated group	481:496	nontreated group (control)	481:506	A total of 144 one-day-old broiler chicks were randomly assigned into 3 groups: nontreated group (control), cyclophosphamide (CPM)-treated groups fed either a basal diet or the diets containing 900 mg/kg IAPS, respectively.
33357691	5	26	theme	Shannon	1227:1233	arg1	index					1235:1239	broiler ADG, Shannon index	1214:1239	broiler ADG, Shannon index	1214:1239	Dietary IAPS supplementation increased broiler ADG, Shannon index, and decreased Simpson index (P < 0.05).
33357691	2	27	theme	one-day-old	416:426	arg1	chicks					436:441	144 one-day-old broiler chicks	412:441	144 one-day-old broiler chicks	412:441	A total of 144 one-day-old broiler chicks were randomly assigned into 3 groups: nontreated group (control), cyclophosphamide (CPM)-treated groups fed either a basal diet or the diets containing 900 mg/kg IAPS, respectively.
33357691	0	28	theme	Microbiota	0:9	arg1	populations					11:21	Microbiota populations	0:21	Microbiota populations	0:21	Microbiota populations and short-chain fatty acids production in cecum of immunosuppressed broilers consuming diets containing γ-irradiated Astragalus polysaccharides.
33357691	3	29	dep	CPM	834:836	arg1	40 mg/kg					839:846	40 mg/kg	839:846	40 mg/kg of BW	839:852	On day 16, 18, and 20, broilers in the control group were intramuscularly injected with 0.5 mL sterilized saline (0.75%, wt/vol), and those in the CPM and IAPS groups were intramuscularly injected with 0.5 mL CPM (40 mg/kg of BW).
33357691	7	30	theme	IAPS	1562:1565	arg1	treatment					1567:1575	The IAPS treatment	1558:1575	The IAPS treatment	1558:1575	The IAPS treatment also increased butyrate concentration (P < 0.05) and tended to elevate acetate concentration (P = 0.052) in cecal digesta.
33357691	4	31	theme	acetate	1108:1114	arg1	concentrations					1084:1097	the concentrations	1080:1097	the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05)	1080:1172	The trial lasted 21 d. Compared with the control group, CPM treatment decreased the broiler average daily gain (ADG) and feed intake (P < 0.05) but did not affect the overall microbial diversity and compositions, as well as the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05).
33357691	5	32	theme	IAPS	1183:1186	arg1	supplementation					1188:1202	Dietary IAPS supplementation	1175:1202	Dietary IAPS supplementation	1175:1202	Dietary IAPS supplementation increased broiler ADG, Shannon index, and decreased Simpson index (P < 0.05).
33357691	9	33	theme	Astragalus	1986:1995	arg1	polysaccharides					1997:2011	modified Astragalus polysaccharides	1977:2011	modified Astragalus polysaccharides	1977:2011	These findings may expand our knowledge about the function of modified Astragalus polysaccharides in broiler chickens.
33357691	2	34	contain	containing	584:593	arg1	diets					578:582	the diets	574:582	the diets containing 900 mg/kg IAPS	574:608	A total of 144 one-day-old broiler chicks were randomly assigned into 3 groups: nontreated group (control), cyclophosphamide (CPM)-treated groups fed either a basal diet or the diets containing 900 mg/kg IAPS, respectively.
33357691	2	34	contain	containing	584:593	arg2	IAPS					605:608	900 mg/kg IAPS	595:608	900 mg/kg IAPS	595:608	A total of 144 one-day-old broiler chicks were randomly assigned into 3 groups: nontreated group (control), cyclophosphamide (CPM)-treated groups fed either a basal diet or the diets containing 900 mg/kg IAPS, respectively.
33357691	0	35	theme	fatty	39:43	arg1	acids					45:49	short-chain fatty acids	27:49	short-chain fatty acids	27:49	Microbiota populations and short-chain fatty acids production in cecum of immunosuppressed broilers consuming diets containing γ-irradiated Astragalus polysaccharides.
33357691	4	36	theme	broiler	940:946	arg1	ADG					968:970	ADG	968:970	ADG	968:970	The trial lasted 21 d. Compared with the control group, CPM treatment decreased the broiler average daily gain (ADG) and feed intake (P < 0.05) but did not affect the overall microbial diversity and compositions, as well as the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05).
33357691	4	36	theme	broiler	940:946	arg1	gain					962:965	the broiler average daily gain	936:965	the broiler average daily gain (ADG)	936:971	The trial lasted 21 d. Compared with the control group, CPM treatment decreased the broiler average daily gain (ADG) and feed intake (P < 0.05) but did not affect the overall microbial diversity and compositions, as well as the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05).
33357691	1	37	theme	short-chain	347:357	arg1	acids					365:369	cecal short-chain fatty acids	341:369	cecal short-chain fatty acids of immunosuppressed broilers	341:398	This study was designed to evaluate the effects of γ-irradiated Astragalus polysaccharides (IAPS) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers.
33357691	5	38	theme	Simpson	1256:1262	arg1	index					1264:1268	Simpson index	1256:1268	Simpson index (P < 0.05)	1256:1279	Dietary IAPS supplementation increased broiler ADG, Shannon index, and decreased Simpson index (P < 0.05).
33357691	5	38	theme	Simpson	1256:1262	arg1	P < 0.05					1271:1278	P < 0.05	1271:1278	P < 0.05	1271:1278	Dietary IAPS supplementation increased broiler ADG, Shannon index, and decreased Simpson index (P < 0.05).
33357691	3	39	from	broilers	648:655	arg1	group					672:676	the control group	660:676	the control group	660:676	On day 16, 18, and 20, broilers in the control group were intramuscularly injected with 0.5 mL sterilized saline (0.75%, wt/vol), and those in the CPM and IAPS groups were intramuscularly injected with 0.5 mL CPM (40 mg/kg of BW).
33357691	8	40	theme	immunosuppressive	1887:1903	arg1	broilers					1905:1912	immunosuppressive broilers	1887:1912	immunosuppressive broilers	1887:1912	The results indicated that IAPS are effective in increasing the cecal beneficial bacteria and short-chain fatty acids production, contributing to improvement in the growth performance of immunosuppressive broilers.
33357691	6	41	theme	lower	1338:1342	arg1	abundances					1344:1353	lower abundances	1338:1353	lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus	1338:1406	Specifically, broilers fed diets containing IAPS showed lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus and higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05).
33357691	4	42	theme	feed	977:980	arg1	P < 0.05					990:997	P < 0.05	990:997	P < 0.05	990:997	The trial lasted 21 d. Compared with the control group, CPM treatment decreased the broiler average daily gain (ADG) and feed intake (P < 0.05) but did not affect the overall microbial diversity and compositions, as well as the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05).
33357691	4	42	theme	feed	977:980	arg1	intake					982:987	feed intake	977:987	feed intake (P < 0.05)	977:998	The trial lasted 21 d. Compared with the control group, CPM treatment decreased the broiler average daily gain (ADG) and feed intake (P < 0.05) but did not affect the overall microbial diversity and compositions, as well as the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05).
33357691	3	43	theme	control	664:670	arg1	group					672:676	the control group	660:676	the control group	660:676	On day 16, 18, and 20, broilers in the control group were intramuscularly injected with 0.5 mL sterilized saline (0.75%, wt/vol), and those in the CPM and IAPS groups were intramuscularly injected with 0.5 mL CPM (40 mg/kg of BW).
33357691	8	44	theme	beneficial	1770:1779	arg1	bacteria					1781:1788	the cecal beneficial bacteria	1760:1788	the cecal beneficial bacteria	1760:1788	The results indicated that IAPS are effective in increasing the cecal beneficial bacteria and short-chain fatty acids production, contributing to improvement in the growth performance of immunosuppressive broilers.
33357691	1	45	theme	cecal	289:293	arg1	populations					306:316	cecal microbiota populations	289:316	cecal microbiota populations	289:316	This study was designed to evaluate the effects of γ-irradiated Astragalus polysaccharides (IAPS) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers.
33357691	2	46	theme	900 mg/kg	595:603	arg1	IAPS					605:608	900 mg/kg IAPS	595:608	900 mg/kg IAPS	595:608	A total of 144 one-day-old broiler chicks were randomly assigned into 3 groups: nontreated group (control), cyclophosphamide (CPM)-treated groups fed either a basal diet or the diets containing 900 mg/kg IAPS, respectively.
33357691	4	47	theme	overall	1023:1029	arg1	diversity					1041:1049	the overall microbial diversity	1019:1049	the overall microbial diversity	1019:1049	The trial lasted 21 d. Compared with the control group, CPM treatment decreased the broiler average daily gain (ADG) and feed intake (P < 0.05) but did not affect the overall microbial diversity and compositions, as well as the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05).
33357691	3	48	theme	0.5 mL	713:718	arg1	saline					731:736	0.5 mL sterilized saline	713:736	0.5 mL sterilized saline (0.75%, wt/vol)	713:752	On day 16, 18, and 20, broilers in the control group were intramuscularly injected with 0.5 mL sterilized saline (0.75%, wt/vol), and those in the CPM and IAPS groups were intramuscularly injected with 0.5 mL CPM (40 mg/kg of BW).
33357691	3	48	theme	0.5 mL	713:718	arg1	%					743:743	0.75%	739:743	0.75%	739:743	On day 16, 18, and 20, broilers in the control group were intramuscularly injected with 0.5 mL sterilized saline (0.75%, wt/vol), and those in the CPM and IAPS groups were intramuscularly injected with 0.5 mL CPM (40 mg/kg of BW).
33357691	8	49	theme	fatty	1806:1810	arg1	acids					1812:1816	short-chain fatty acids	1794:1816	short-chain fatty acids	1794:1816	The results indicated that IAPS are effective in increasing the cecal beneficial bacteria and short-chain fatty acids production, contributing to improvement in the growth performance of immunosuppressive broilers.
33357691	6	50	theme	higher	1412:1417	arg1	proportions					1419:1429	higher proportions	1412:1429	higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05)	1412:1555	Specifically, broilers fed diets containing IAPS showed lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus and higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05).
33357691	6	51	dep	Ruminococcaceae	1434:1448	arg1	RF39_norank					1520:1530	RF39_norank	1520:1530	RF39_norank	1520:1530	Specifically, broilers fed diets containing IAPS showed lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus and higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05).
33357691	6	52	theme	Butyricicoccus	1393:1406	arg1	proportions					1419:1429	higher proportions	1412:1429	higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05)	1412:1555	Specifically, broilers fed diets containing IAPS showed lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus and higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05).
33357691	6	52	theme	Butyricicoccus	1393:1406	arg1	abundances					1344:1353	lower abundances	1338:1353	lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus	1338:1406	Specifically, broilers fed diets containing IAPS showed lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus and higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05).
33357691	1	53	theme	γ-irradiated	219:230	arg1	IAPS					260:263	IAPS	260:263	IAPS	260:263	This study was designed to evaluate the effects of γ-irradiated Astragalus polysaccharides (IAPS) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers.
33357691	1	53	theme	γ-irradiated	219:230	arg1	polysaccharides					243:257	γ-irradiated Astragalus polysaccharides	219:257	γ-irradiated Astragalus polysaccharides (IAPS)	219:264	This study was designed to evaluate the effects of γ-irradiated Astragalus polysaccharides (IAPS) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers.
33357691	2	54	theme	basal	560:564	arg1	diet					566:569	a basal diet	558:569	a basal diet	558:569	A total of 144 one-day-old broiler chicks were randomly assigned into 3 groups: nontreated group (control), cyclophosphamide (CPM)-treated groups fed either a basal diet or the diets containing 900 mg/kg IAPS, respectively.
33357691	1	55	theme	polysaccharides	243:257	arg1	effects					208:214	the effects	204:214	the effects of γ-irradiated Astragalus polysaccharides (IAPS) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers	204:398	This study was designed to evaluate the effects of γ-irradiated Astragalus polysaccharides (IAPS) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers.
33357691	6	56	theme	Sellimonas	1493:1502	arg1	proportions					1419:1429	higher proportions	1412:1429	higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05)	1412:1555	Specifically, broilers fed diets containing IAPS showed lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus and higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05).
33357691	6	56	theme	Sellimonas	1493:1502	arg1	abundances					1344:1353	lower abundances	1338:1353	lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus	1338:1406	Specifically, broilers fed diets containing IAPS showed lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus and higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05).
33357691	7	57	theme	acetate	1648:1654	arg1	P = 0.052					1671:1679	P = 0.052	1671:1679	P = 0.052	1671:1679	The IAPS treatment also increased butyrate concentration (P < 0.05) and tended to elevate acetate concentration (P = 0.052) in cecal digesta.
33357691	7	57	theme	acetate	1648:1654	arg1	concentration					1656:1668	acetate concentration	1648:1668	acetate concentration (P = 0.052)	1648:1680	The IAPS treatment also increased butyrate concentration (P < 0.05) and tended to elevate acetate concentration (P = 0.052) in cecal digesta.
33357691	6	58	theme	Negativibacillus	1459:1474	arg1	proportions					1419:1429	higher proportions	1412:1429	higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05)	1412:1555	Specifically, broilers fed diets containing IAPS showed lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus and higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05).
33357691	6	58	theme	Negativibacillus	1459:1474	arg1	abundances					1344:1353	lower abundances	1338:1353	lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus	1338:1406	Specifically, broilers fed diets containing IAPS showed lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus and higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05).
33357691	4	59	theme	control	897:903	arg1	group					905:909	the control group	893:909	the control group	893:909	The trial lasted 21 d. Compared with the control group, CPM treatment decreased the broiler average daily gain (ADG) and feed intake (P < 0.05) but did not affect the overall microbial diversity and compositions, as well as the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05).
33357691	0	60	theme	broilers	91:98	arg1	cecum					65:69	cecum	65:69	cecum of immunosuppressed broilers consuming diets containing γ-irradiated Astragalus polysaccharides	65:165	Microbiota populations and short-chain fatty acids production in cecum of immunosuppressed broilers consuming diets containing γ-irradiated Astragalus polysaccharides.
33357691	8	61	theme	acids	1812:1816	arg1	production					1818:1827	the cecal beneficial bacteria and short-chain fatty acids production	1760:1827	the cecal beneficial bacteria and short-chain fatty acids production	1760:1827	The results indicated that IAPS are effective in increasing the cecal beneficial bacteria and short-chain fatty acids production, contributing to improvement in the growth performance of immunosuppressive broilers.
33357691	3	62	theme	IAPS	780:783	arg1	groups					785:790	the CPM and IAPS groups	768:790	the CPM and IAPS groups	768:790	On day 16, 18, and 20, broilers in the control group were intramuscularly injected with 0.5 mL sterilized saline (0.75%, wt/vol), and those in the CPM and IAPS groups were intramuscularly injected with 0.5 mL CPM (40 mg/kg of BW).
33357691	4	63	dep	lasted	866:871	arg1	21 d.					873:877	21 d.	873:877	lasted 21 d. Compared with the control group	866:909	The trial lasted 21 d. Compared with the control group, CPM treatment decreased the broiler average daily gain (ADG) and feed intake (P < 0.05) but did not affect the overall microbial diversity and compositions, as well as the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05).
33357691	1	64	theme	broilers	391:398	arg1	acids					365:369	cecal short-chain fatty acids	341:369	cecal short-chain fatty acids of immunosuppressed broilers	341:398	This study was designed to evaluate the effects of γ-irradiated Astragalus polysaccharides (IAPS) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers.
33357691	3	65	theme	0.5 mL	827:832	arg1	CPM					834:836	0.5 mL CPM	827:836	0.5 mL CPM (40 mg/kg of BW)	827:853	On day 16, 18, and 20, broilers in the control group were intramuscularly injected with 0.5 mL sterilized saline (0.75%, wt/vol), and those in the CPM and IAPS groups were intramuscularly injected with 0.5 mL CPM (40 mg/kg of BW).
33357691	4	66	theme	butyrate	1133:1140	arg1	concentrations					1084:1097	the concentrations	1080:1097	the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05)	1080:1172	The trial lasted 21 d. Compared with the control group, CPM treatment decreased the broiler average daily gain (ADG) and feed intake (P < 0.05) but did not affect the overall microbial diversity and compositions, as well as the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05).
33357691	5	67	theme	broiler	1214:1220	arg1	index					1235:1239	broiler ADG, Shannon index	1214:1239	broiler ADG, Shannon index	1214:1239	Dietary IAPS supplementation increased broiler ADG, Shannon index, and decreased Simpson index (P < 0.05).
33357691	3	68	theme	CPM	772:774	arg1	groups					785:790	the CPM and IAPS groups	768:790	the CPM and IAPS groups	768:790	On day 16, 18, and 20, broilers in the control group were intramuscularly injected with 0.5 mL sterilized saline (0.75%, wt/vol), and those in the CPM and IAPS groups were intramuscularly injected with 0.5 mL CPM (40 mg/kg of BW).
33357691	0	69	theme	γ-irradiated	127:138	arg1	polysaccharides					151:165	γ-irradiated Astragalus polysaccharides	127:165	γ-irradiated Astragalus polysaccharides	127:165	Microbiota populations and short-chain fatty acids production in cecum of immunosuppressed broilers consuming diets containing γ-irradiated Astragalus polysaccharides.
33357691	3	70	theme	BW	851:852	arg1	40 mg/kg					839:846	40 mg/kg	839:846	40 mg/kg of BW	839:852	On day 16, 18, and 20, broilers in the control group were intramuscularly injected with 0.5 mL sterilized saline (0.75%, wt/vol), and those in the CPM and IAPS groups were intramuscularly injected with 0.5 mL CPM (40 mg/kg of BW).
33357691	2	71	dep	groups	473:478	arg1	control					499:505	control	499:505	control	499:505	A total of 144 one-day-old broiler chicks were randomly assigned into 3 groups: nontreated group (control), cyclophosphamide (CPM)-treated groups fed either a basal diet or the diets containing 900 mg/kg IAPS, respectively.
33357691	2	71	dep	groups	473:478	arg1	group					492:496	nontreated group	481:496	nontreated group (control)	481:506	A total of 144 one-day-old broiler chicks were randomly assigned into 3 groups: nontreated group (control), cyclophosphamide (CPM)-treated groups fed either a basal diet or the diets containing 900 mg/kg IAPS, respectively.
33357691	4	72	theme	cecal	1102:1106	arg1	acetate					1108:1114	cecal acetate	1102:1114	cecal acetate	1102:1114	The trial lasted 21 d. Compared with the control group, CPM treatment decreased the broiler average daily gain (ADG) and feed intake (P < 0.05) but did not affect the overall microbial diversity and compositions, as well as the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05).
33357691	2	73	theme	broiler	428:434	arg1	chicks					436:441	144 one-day-old broiler chicks	412:441	144 one-day-old broiler chicks	412:441	A total of 144 one-day-old broiler chicks were randomly assigned into 3 groups: nontreated group (control), cyclophosphamide (CPM)-treated groups fed either a basal diet or the diets containing 900 mg/kg IAPS, respectively.
33357691	4	74	theme	average	948:954	arg1	ADG					968:970	ADG	968:970	ADG	968:970	The trial lasted 21 d. Compared with the control group, CPM treatment decreased the broiler average daily gain (ADG) and feed intake (P < 0.05) but did not affect the overall microbial diversity and compositions, as well as the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05).
33357691	4	74	theme	average	948:954	arg1	gain					962:965	the broiler average daily gain	936:965	the broiler average daily gain (ADG)	936:971	The trial lasted 21 d. Compared with the control group, CPM treatment decreased the broiler average daily gain (ADG) and feed intake (P < 0.05) but did not affect the overall microbial diversity and compositions, as well as the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05).
33357691	5	75	theme	Dietary	1175:1181	arg1	supplementation					1188:1202	Dietary IAPS supplementation	1175:1202	Dietary IAPS supplementation	1175:1202	Dietary IAPS supplementation increased broiler ADG, Shannon index, and decreased Simpson index (P < 0.05).
33357691	0	76	theme	short-chain	27:37	arg1	acids					45:49	short-chain fatty acids	27:49	short-chain fatty acids	27:49	Microbiota populations and short-chain fatty acids production in cecum of immunosuppressed broilers consuming diets containing γ-irradiated Astragalus polysaccharides.
33357691	9	77	theme	modified	1977:1984	arg1	polysaccharides					1997:2011	modified Astragalus polysaccharides	1977:2011	modified Astragalus polysaccharides	1977:2011	These findings may expand our knowledge about the function of modified Astragalus polysaccharides in broiler chickens.
33357691	4	78	from	concentrations	1084:1097	arg1	cecum					1145:1149	cecum	1145:1149	cecum of broilers (P > 0.05)	1145:1172	The trial lasted 21 d. Compared with the control group, CPM treatment decreased the broiler average daily gain (ADG) and feed intake (P < 0.05) but did not affect the overall microbial diversity and compositions, as well as the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05).
33357691	9	79	theme	polysaccharides	1997:2011	arg1	function					1965:1972	the function	1961:1972	the function of modified Astragalus polysaccharides in broiler chickens	1961:2031	These findings may expand our knowledge about the function of modified Astragalus polysaccharides in broiler chickens.
33357691	0	80	contain	containing	116:125	arg1	diets					110:114	diets	110:114	diets containing γ-irradiated Astragalus polysaccharides	110:165	Microbiota populations and short-chain fatty acids production in cecum of immunosuppressed broilers consuming diets containing γ-irradiated Astragalus polysaccharides.
33357691	0	80	contain	containing	116:125	arg2	polysaccharides					151:165	γ-irradiated Astragalus polysaccharides	127:165	γ-irradiated Astragalus polysaccharides	127:165	Microbiota populations and short-chain fatty acids production in cecum of immunosuppressed broilers consuming diets containing γ-irradiated Astragalus polysaccharides.
33357691	1	81	theme	cecal	341:345	arg1	acids					365:369	cecal short-chain fatty acids	341:369	cecal short-chain fatty acids of immunosuppressed broilers	341:398	This study was designed to evaluate the effects of γ-irradiated Astragalus polysaccharides (IAPS) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers.
33357691	9	82	from	function	1965:1972	arg1	chickens					2024:2031	broiler chickens	2016:2031	broiler chickens	2016:2031	These findings may expand our knowledge about the function of modified Astragalus polysaccharides in broiler chickens.
33357691	9	83	theme	broiler	2016:2022	arg1	chickens					2024:2031	broiler chickens	2016:2031	broiler chickens	2016:2031	These findings may expand our knowledge about the function of modified Astragalus polysaccharides in broiler chickens.
33357691	6	84	theme	Bacteroides	1376:1386	arg1	proportions					1419:1429	higher proportions	1412:1429	higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05)	1412:1555	Specifically, broilers fed diets containing IAPS showed lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus and higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05).
33357691	6	84	theme	Bacteroides	1376:1386	arg1	abundances					1344:1353	lower abundances	1338:1353	lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus	1338:1406	Specifically, broilers fed diets containing IAPS showed lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus and higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05).
33357691	1	85	theme	fatty	359:363	arg1	acids					365:369	cecal short-chain fatty acids	341:369	cecal short-chain fatty acids of immunosuppressed broilers	341:398	This study was designed to evaluate the effects of γ-irradiated Astragalus polysaccharides (IAPS) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers.
33357691	6	86	contain	containing	1315:1324	arg2	IAPS					1326:1329	IAPS	1326:1329	IAPS	1326:1329	Specifically, broilers fed diets containing IAPS showed lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus and higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05).
33357691	6	86	contain	containing	1315:1324	arg1	diets					1309:1313	diets	1309:1313	diets containing IAPS	1309:1329	Specifically, broilers fed diets containing IAPS showed lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus and higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05).
33357691	4	87	theme	broilers	1154:1161	arg1	cecum					1145:1149	cecum	1145:1149	cecum of broilers (P > 0.05)	1145:1172	The trial lasted 21 d. Compared with the control group, CPM treatment decreased the broiler average daily gain (ADG) and feed intake (P < 0.05) but did not affect the overall microbial diversity and compositions, as well as the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05).
33357691	6	88	theme	Faecalibacterium	1358:1373	arg1	proportions					1419:1429	higher proportions	1412:1429	higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05)	1412:1555	Specifically, broilers fed diets containing IAPS showed lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus and higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05).
33357691	6	88	theme	Faecalibacterium	1358:1373	arg1	abundances					1344:1353	lower abundances	1338:1353	lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus	1338:1406	Specifically, broilers fed diets containing IAPS showed lower abundances of Faecalibacterium, Bacteroides, and Butyricicoccus and higher proportions of Ruminococcaceae UCG-014, Negativibacillus, Shuttleworthia, Sellimonas, and Mollicutes RF39_norank, respectively (P < 0.05).
33357691	4	89	theme	daily	956:960	arg1	ADG					968:970	ADG	968:970	ADG	968:970	The trial lasted 21 d. Compared with the control group, CPM treatment decreased the broiler average daily gain (ADG) and feed intake (P < 0.05) but did not affect the overall microbial diversity and compositions, as well as the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05).
33357691	4	89	theme	daily	956:960	arg1	gain					962:965	the broiler average daily gain	936:965	the broiler average daily gain (ADG)	936:971	The trial lasted 21 d. Compared with the control group, CPM treatment decreased the broiler average daily gain (ADG) and feed intake (P < 0.05) but did not affect the overall microbial diversity and compositions, as well as the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers (P > 0.05).
33357691	1	90	theme	growth	269:274	arg1	performance					276:286	growth performance	269:286	growth performance	269:286	This study was designed to evaluate the effects of γ-irradiated Astragalus polysaccharides (IAPS) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers.
33604750	2	0	theme	diffraction	483:493	arg1	pattern					495:501	X-ray diffraction pattern	477:501	X-ray diffraction pattern	477:501	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	1	theme	isolated	596:603	arg1	bacterium					618:626	the locally isolated thermophilic bacterium	584:626	the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646)	584:670	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	8	2	theme	ester	1164:1168	arg1	presence					1144:1151	the presence	1140:1151	the presence of sulfate ester (band at 1217 cm-1), an indication of the antitumor effect	1140:1227	The FTIR analysis confirmed the presence of sulfate ester (band at 1217 cm-1), an indication of the antitumor effect.
33604750	6	3	dep	%	1045:1045	arg1	protein					1047:1053	protein	1047:1053	1.08% protein	1041:1053	EPS contained 93% carbohydrates and 1.08% protein.
33604750	2	4	theme	X-ray	477:481	arg1	pattern					495:501	X-ray diffraction pattern	477:501	X-ray diffraction pattern	477:501	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	11	5	theme	pushchinoensis	1465:1478	arg1	activity					1450:1457	the biological activity	1435:1457	the biological activity of A. pushchinoensis	1435:1478	This is the first report on the EPS production potential and the biological activity of A. pushchinoensis.
33604750	11	5	theme	pushchinoensis	1465:1478	arg1	production					1410:1419	the EPS production potential and the biological activity of A. pushchinoensis	1402:1478	production	1410:1419	This is the first report on the EPS production potential and the biological activity of A. pushchinoensis.
33604750	1	6	theme	pharmaceutical	179:192	arg1	industries					194:203	the food and pharmaceutical industries	166:203	the food and pharmaceutical industries	166:203	Exopolysaccharides (EPS/EPSs) possess several various applications in the food and pharmaceutical industries.
33604750	8	7	theme	at	1176:1177	arg1	1217 cm-1					1179:1187	band at 1217 cm-1	1171:1187	band at 1217 cm-1	1171:1187	The FTIR analysis confirmed the presence of sulfate ester (band at 1217 cm-1), an indication of the antitumor effect.
33604750	8	7	theme	at	1176:1177	arg1	ester					1164:1168	sulfate ester	1156:1168	sulfate ester (band at 1217 cm-1)	1156:1188	The FTIR analysis confirmed the presence of sulfate ester (band at 1217 cm-1), an indication of the antitumor effect.
33604750	4	8	theme	EPS	801:803	arg1	viscosity					788:796	The viscosity	784:796	The viscosity of EPS showing Newtonian flow	784:826	The viscosity of EPS showing Newtonian flow was temperature dependent.
33604750	4	8	theme	EPS	801:803	arg1	dependent					844:852	dependent	844:852	dependent	844:852	The viscosity of EPS showing Newtonian flow was temperature dependent.
33604750	2	9	dep	solubility	355:364	arg1	content					392:398	carbohydrate and protein content	367:398	content	392:398	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	9	dep	solubility	355:364	arg1	microscopy					525:534	scanning electron microscopy	507:534	scanning electron microscopy	507:534	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	9	dep	solubility	355:364	arg1	analysis					449:456	functional group analysis	432:456	functional group analysis	432:456	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	9	dep	solubility	355:364	arg1	composition					401:411	composition	401:411	composition	401:411	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	9	dep	solubility	355:364	arg1	pattern					495:501	X-ray diffraction pattern	477:501	X-ray diffraction pattern	477:501	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	9	dep	solubility	355:364	arg1	weight					424:429	molecular weight	414:429	molecular weight	414:429	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	9	dep	solubility	355:364	arg1	analysis					467:474	thermal analysis	459:474	thermal analysis	459:474	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	7	10	theme	EPS	1080:1082	arg1	weight					1070:1075	The molecular weight	1056:1075	The molecular weight of EPS	1056:1082	The molecular weight of EPS was determined as 75.5 kDa.
33604750	7	10	theme	EPS	1080:1082	arg1	75.5 kDa					1102:1109	75.5 kDa	1102:1109	75.5 kDa	1102:1109	The molecular weight of EPS was determined as 75.5 kDa.
33604750	11	11	dep	production	1410:1419	arg1	potential					1421:1429	potential	1421:1429	potential	1421:1429	This is the first report on the EPS production potential and the biological activity of A. pushchinoensis.
33604750	2	12	theme	electron	516:523	arg1	microscopy					525:534	scanning electron microscopy	507:534	scanning electron microscopy	507:534	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	13	theme	chemical	345:352	arg1	properties					537:546	the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties	246:546	the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646)	246:670	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	4	14	theme	Newtonian	813:821	arg1	flow					823:826	Newtonian flow	813:826	Newtonian flow	813:826	The viscosity of EPS showing Newtonian flow was temperature dependent.
33604750	0	15	contain	has	54:56	arg1	Exopolysaccharide					0:16	Exopolysaccharide	0:16	Exopolysaccharide of Anoxybacillus pushchinoensis G11	0:52	Exopolysaccharide of Anoxybacillus pushchinoensis G11 has antitumor and antibiofilm activities.
33604750	0	15	contain	has	54:56	arg2	activities					84:93	antitumor and antibiofilm activities	58:93	activities	84:93	Exopolysaccharide of Anoxybacillus pushchinoensis G11 has antitumor and antibiofilm activities.
33604750	6	16	contain	contained	1009:1017	arg1	EPS					1005:1007	EPS	1005:1007	EPS	1005:1007	EPS contained 93% carbohydrates and 1.08% protein.
33604750	6	16	contain	contained	1009:1017	arg2	%					1045:1045	1.08%	1041:1045	1.08% protein	1041:1053	EPS contained 93% carbohydrates and 1.08% protein.
33604750	6	16	contain	contained	1009:1017	arg2	%					1021:1021	93%	1019:1021	93% carbohydrates	1019:1035	EPS contained 93% carbohydrates and 1.08% protein.
33604750	2	17	theme	scanning	507:514	arg1	microscopy					525:534	scanning electron microscopy	507:534	scanning electron microscopy	507:534	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	1	18	from	applications	150:161	arg1	industries					194:203	the food and pharmaceutical industries	166:203	the food and pharmaceutical industries	166:203	Exopolysaccharides (EPS/EPSs) possess several various applications in the food and pharmaceutical industries.
33604750	2	19	theme	rheological	290:300	arg1	properties					537:546	the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties	246:546	the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646)	246:670	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	6	20	dep	%	1021:1021	arg1	carbohydrates					1023:1035	carbohydrates	1023:1035	93% carbohydrates	1019:1035	EPS contained 93% carbohydrates and 1.08% protein.
33604750	2	21	dep	bacterium	618:626	arg1	MN720646					662:669	MN720646	662:669	MN720646	662:669	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	21	dep	bacterium	618:626	arg1	G11					657:659	G11	657:659	G11	657:659	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	10	22	theme	weight	1289:1294	arg1	%					1280:1280	36%	1278:1280	36% of its weight	1278:1294	It could maintain 36% of its weight at 800 °C and crystallization and melting temperatures were 221 and 255.6 °C.
33604750	10	22	theme	weight	1289:1294	arg1	weight					1289:1294	its weight	1285:1294	its weight	1285:1294	It could maintain 36% of its weight at 800 °C and crystallization and melting temperatures were 221 and 255.6 °C.
33604750	11	23	theme	EPS	1406:1408	arg1	production					1410:1419	the EPS production potential and the biological activity of A. pushchinoensis	1402:1478	production	1410:1419	This is the first report on the EPS production potential and the biological activity of A. pushchinoensis.
33604750	8	24	theme	effect	1222:1227	arg1	indication					1194:1203	an indication	1191:1203	an indication of the antitumor effect	1191:1227	The FTIR analysis confirmed the presence of sulfate ester (band at 1217 cm-1), an indication of the antitumor effect.
33604750	8	24	theme	effect	1222:1227	arg1	ester					1164:1168	sulfate ester	1156:1168	sulfate ester (band at 1217 cm-1)	1156:1188	The FTIR analysis confirmed the presence of sulfate ester (band at 1217 cm-1), an indication of the antitumor effect.
33604750	2	25	theme	protein	384:390	arg1	content					392:398	carbohydrate and protein content	367:398	content	392:398	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	25	theme	protein	384:390	arg1	composition					401:411	composition	401:411	composition	401:411	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	25	theme	protein	384:390	arg1	weight					424:429	molecular weight	414:429	molecular weight	414:429	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	26	theme	group	443:447	arg1	analysis					449:456	functional group analysis	432:456	functional group analysis	432:456	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	3	27	theme	colon	739:743	arg1	activities					772:781	antitumor [lung (A-549) and colon (Caco-2 and HT-29) cancer] activities	711:781	activities	772:781	EPS was found to have antibiofilm and antitumor [lung (A-549) and colon (Caco-2 and HT-29) cancer] activities.
33604750	3	27	theme	colon	739:743	arg1	HT-29					757:761	HT-29	757:761	HT-29	757:761	EPS was found to have antibiofilm and antitumor [lung (A-549) and colon (Caco-2 and HT-29) cancer] activities.
33604750	3	27	theme	colon	739:743	arg1	Caco-2					746:751	Caco-2	746:751	Caco-2	746:751	EPS was found to have antibiofilm and antitumor [lung (A-549) and colon (Caco-2 and HT-29) cancer] activities.
33604750	2	28	theme	thermophilic	605:616	arg1	bacterium					618:626	the locally isolated thermophilic bacterium	584:626	the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646)	584:670	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	8	29	from	1217 cm-1	1179:1187	arg1	band					1171:1174	band at 1217 cm-1	1171:1187	band at 1217 cm-1	1171:1187	The FTIR analysis confirmed the presence of sulfate ester (band at 1217 cm-1), an indication of the antitumor effect.
33604750	8	29	from	1217 cm-1	1179:1187	arg1	at					1176:1177	band at 1217 cm-1	1171:1187	band at 1217 cm-1	1171:1187	The FTIR analysis confirmed the presence of sulfate ester (band at 1217 cm-1), an indication of the antitumor effect.
33604750	8	30	theme	antitumor	1212:1220	arg1	effect					1222:1227	the antitumor effect	1208:1227	the antitumor effect	1208:1227	The FTIR analysis confirmed the presence of sulfate ester (band at 1217 cm-1), an indication of the antitumor effect.
33604750	5	31	contain	containing	926:935	arg2	%					1001:1001	5%	1000:1001	5%	1000:1001	As chemical properties, the EPS was found to be a heteropolysaccharide containing arabinose (57%), fructose (26%), glucose (12%), and galactose (5%).
33604750	5	31	contain	containing	926:935	arg2	galactose					989:997	galactose	989:997	galactose (5%)	989:1002	As chemical properties, the EPS was found to be a heteropolysaccharide containing arabinose (57%), fructose (26%), glucose (12%), and galactose (5%).
33604750	5	31	contain	containing	926:935	arg2	%					950:950	57%	948:950	57%	948:950	As chemical properties, the EPS was found to be a heteropolysaccharide containing arabinose (57%), fructose (26%), glucose (12%), and galactose (5%).
33604750	5	31	contain	containing	926:935	arg2	arabinose					937:945	arabinose	937:945	arabinose (57%)	937:951	As chemical properties, the EPS was found to be a heteropolysaccharide containing arabinose (57%), fructose (26%), glucose (12%), and galactose (5%).
33604750	5	31	contain	containing	926:935	arg2	fructose					954:961	fructose	954:961	fructose (26%)	954:967	As chemical properties, the EPS was found to be a heteropolysaccharide containing arabinose (57%), fructose (26%), glucose (12%), and galactose (5%).
33604750	5	31	contain	containing	926:935	arg1	heteropolysaccharide					905:924	a heteropolysaccharide	903:924	a heteropolysaccharide containing arabinose (57%), fructose (26%), glucose (12%), and galactose (5%)	903:1002	As chemical properties, the EPS was found to be a heteropolysaccharide containing arabinose (57%), fructose (26%), glucose (12%), and galactose (5%).
33604750	5	31	contain	containing	926:935	arg2	glucose					970:976	glucose	970:976	glucose (12%)	970:982	As chemical properties, the EPS was found to be a heteropolysaccharide containing arabinose (57%), fructose (26%), glucose (12%), and galactose (5%).
33604750	5	31	contain	containing	926:935	arg2	%					966:966	26%	964:966	26%	964:966	As chemical properties, the EPS was found to be a heteropolysaccharide containing arabinose (57%), fructose (26%), glucose (12%), and galactose (5%).
33604750	5	31	contain	containing	926:935	arg2	%					981:981	12%	979:981	12%	979:981	As chemical properties, the EPS was found to be a heteropolysaccharide containing arabinose (57%), fructose (26%), glucose (12%), and galactose (5%).
33604750	5	31	contain	containing	926:935	arg1	EPS					883:885	the EPS	879:885	the EPS	879:885	As chemical properties, the EPS was found to be a heteropolysaccharide containing arabinose (57%), fructose (26%), glucose (12%), and galactose (5%).
33604750	8	32	theme	FTIR	1116:1119	arg1	analysis					1121:1128	The FTIR analysis	1112:1128	The FTIR analysis	1112:1128	The FTIR analysis confirmed the presence of sulfate ester (band at 1217 cm-1), an indication of the antitumor effect.
33604750	2	33	theme	functional	432:441	arg1	analysis					449:456	functional group analysis	432:456	functional group analysis	432:456	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	34	theme	EPS	555:557	arg1	properties					537:546	the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties	246:546	the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646)	246:670	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	35	dep	chemical	345:352	arg1	solubility					355:364	solubility	355:364	solubility	355:364	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	36	dep	biological	250:259	arg1	antitumor					278:286	antitumor	278:286	antitumor	278:286	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	36	dep	biological	250:259	arg1	antibiofilm					262:272	antibiofilm	262:272	antibiofilm	262:272	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	37	theme	shear	316:320	arg1	rate					322:325	shear rate	316:325	shear rate	316:325	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	38	theme	molecular	414:422	arg1	content					392:398	carbohydrate and protein content	367:398	content	392:398	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	38	theme	molecular	414:422	arg1	weight					424:429	molecular weight	414:429	molecular weight	414:429	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	8	39	theme	sulfate	1156:1162	arg1	1217 cm-1					1179:1187	band at 1217 cm-1	1171:1187	band at 1217 cm-1	1171:1187	The FTIR analysis confirmed the presence of sulfate ester (band at 1217 cm-1), an indication of the antitumor effect.
33604750	8	39	theme	sulfate	1156:1162	arg1	ester					1164:1168	sulfate ester	1156:1168	sulfate ester (band at 1217 cm-1)	1156:1188	The FTIR analysis confirmed the presence of sulfate ester (band at 1217 cm-1), an indication of the antitumor effect.
33604750	8	39	theme	sulfate	1156:1162	arg1	indication					1194:1203	an indication	1191:1203	an indication of the antitumor effect	1191:1227	The FTIR analysis confirmed the presence of sulfate ester (band at 1217 cm-1), an indication of the antitumor effect.
33604750	10	40	theme	melting	1330:1336	arg1	temperatures					1338:1349	melting temperatures	1330:1349	melting temperatures	1330:1349	It could maintain 36% of its weight at 800 °C and crystallization and melting temperatures were 221 and 255.6 °C.
33604750	11	41	from	report	1392:1397	arg1	activity					1450:1457	the biological activity	1435:1457	the biological activity of A. pushchinoensis	1435:1478	This is the first report on the EPS production potential and the biological activity of A. pushchinoensis.
33604750	11	41	from	report	1392:1397	arg1	production					1410:1419	the EPS production potential and the biological activity of A. pushchinoensis	1402:1478	production	1410:1419	This is the first report on the EPS production potential and the biological activity of A. pushchinoensis.
33604750	0	42	theme	G11	50:52	arg1	Exopolysaccharide					0:16	Exopolysaccharide	0:16	Exopolysaccharide of Anoxybacillus pushchinoensis G11	0:52	Exopolysaccharide of Anoxybacillus pushchinoensis G11 has antitumor and antibiofilm activities.
33604750	1	43	contain	possess	126:132	arg1	EPS/EPSs					116:123	EPS/EPSs	116:123	EPS/EPSs	116:123	Exopolysaccharides (EPS/EPSs) possess several various applications in the food and pharmaceutical industries.
33604750	1	43	contain	possess	126:132	arg1	Exopolysaccharides					96:113	Exopolysaccharides	96:113	Exopolysaccharides (EPS/EPSs)	96:124	Exopolysaccharides (EPS/EPSs) possess several various applications in the food and pharmaceutical industries.
33604750	1	43	contain	possess	126:132	arg2	applications					150:161	several various applications	134:161	several various applications in the food and pharmaceutical industries	134:203	Exopolysaccharides (EPS/EPSs) possess several various applications in the food and pharmaceutical industries.
33604750	3	44	dep	activities	772:781	arg1	activities					772:781	antitumor [lung (A-549) and colon (Caco-2 and HT-29) cancer] activities	711:781	activities	772:781	EPS was found to have antibiofilm and antitumor [lung (A-549) and colon (Caco-2 and HT-29) cancer] activities.
33604750	3	44	dep	activities	772:781	arg1	HT-29					757:761	HT-29	757:761	HT-29	757:761	EPS was found to have antibiofilm and antitumor [lung (A-549) and colon (Caco-2 and HT-29) cancer] activities.
33604750	3	44	dep	activities	772:781	arg1	Caco-2					746:751	Caco-2	746:751	Caco-2	746:751	EPS was found to have antibiofilm and antitumor [lung (A-549) and colon (Caco-2 and HT-29) cancer] activities.
33604750	2	45	theme	thermal	459:465	arg1	analysis					467:474	thermal analysis	459:474	thermal analysis	459:474	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	5	46	theme	chemical	858:865	arg1	properties					867:876	chemical properties	858:876	chemical properties	858:876	As chemical properties, the EPS was found to be a heteropolysaccharide containing arabinose (57%), fructose (26%), glucose (12%), and galactose (5%).
33604750	5	46	theme	chemical	858:865	arg1	EPS					883:885	the EPS	879:885	the EPS	879:885	As chemical properties, the EPS was found to be a heteropolysaccharide containing arabinose (57%), fructose (26%), glucose (12%), and galactose (5%).
33604750	2	47	theme	biological	250:259	arg1	properties					537:546	the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties	246:546	the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646)	246:670	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	1	48	theme	several	134:140	arg1	applications					150:161	several various applications	134:161	several various applications in the food and pharmaceutical industries	134:203	Exopolysaccharides (EPS/EPSs) possess several various applications in the food and pharmaceutical industries.
33604750	0	49	theme	antitumor	58:66	arg1	activities					84:93	antitumor and antibiofilm activities	58:93	activities	84:93	Exopolysaccharide of Anoxybacillus pushchinoensis G11 has antitumor and antibiofilm activities.
33604750	3	50	contain	have	690:693	arg2	A-549					728:732	A-549	728:732	A-549	728:732	EPS was found to have antibiofilm and antitumor [lung (A-549) and colon (Caco-2 and HT-29) cancer] activities.
33604750	3	50	contain	have	690:693	arg1	EPS					673:675	EPS	673:675	EPS	673:675	EPS was found to have antibiofilm and antitumor [lung (A-549) and colon (Caco-2 and HT-29) cancer] activities.
33604750	3	50	contain	have	690:693	arg2	[lung					721:725	[lung	721:725	[lung	721:725	EPS was found to have antibiofilm and antitumor [lung (A-549) and colon (Caco-2 and HT-29) cancer] activities.
33604750	3	50	contain	have	690:693	arg2	antibiofilm					695:705	antibiofilm	695:705	antibiofilm	695:705	EPS was found to have antibiofilm and antitumor [lung (A-549) and colon (Caco-2 and HT-29) cancer] activities.
33604750	1	51	theme	various	142:148	arg1	applications					150:161	several various applications	134:161	several various applications in the food and pharmaceutical industries	134:203	Exopolysaccharides (EPS/EPSs) possess several various applications in the food and pharmaceutical industries.
33604750	11	52	theme	first	1386:1390	arg1	This					1374:1377	This	1374:1377	This	1374:1377	This is the first report on the EPS production potential and the biological activity of A. pushchinoensis.
33604750	11	52	theme	first	1386:1390	arg1	report					1392:1397	the first report	1382:1397	the first report on the EPS production potential and the biological activity of A. pushchinoensis	1382:1478	This is the first report on the EPS production potential and the biological activity of A. pushchinoensis.
33604750	8	53	theme	band	1171:1174	arg1	1217 cm-1					1179:1187	band at 1217 cm-1	1171:1187	band at 1217 cm-1	1171:1187	The FTIR analysis confirmed the presence of sulfate ester (band at 1217 cm-1), an indication of the antitumor effect.
33604750	8	53	theme	band	1171:1174	arg1	ester					1164:1168	sulfate ester	1156:1168	sulfate ester (band at 1217 cm-1)	1156:1188	The FTIR analysis confirmed the presence of sulfate ester (band at 1217 cm-1), an indication of the antitumor effect.
33604750	2	54	dep	rheological	290:300	arg1	temperature					303:313	temperature	303:313	temperature	303:313	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	54	dep	rheological	290:300	arg1	rate					322:325	shear rate	316:325	shear rate	316:325	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	54	dep	rheological	290:300	arg1	density					332:338	density	332:338	density	332:338	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	11	55	theme	A.	1462:1463	arg1	pushchinoensis					1465:1478	A. pushchinoensis	1462:1478	A. pushchinoensis	1462:1478	This is the first report on the EPS production potential and the biological activity of A. pushchinoensis.
33604750	2	56	theme	carbohydrate	367:378	arg1	content					392:398	carbohydrate and protein content	367:398	content	392:398	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	56	theme	carbohydrate	367:378	arg1	composition					401:411	composition	401:411	composition	401:411	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	2	56	theme	carbohydrate	367:378	arg1	weight					424:429	molecular weight	414:429	molecular weight	414:429	This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646).
33604750	3	57	theme	cancer	764:769	arg1	activities					772:781	antitumor [lung (A-549) and colon (Caco-2 and HT-29) cancer] activities	711:781	activities	772:781	EPS was found to have antibiofilm and antitumor [lung (A-549) and colon (Caco-2 and HT-29) cancer] activities.
33604750	3	57	theme	cancer	764:769	arg1	HT-29					757:761	HT-29	757:761	HT-29	757:761	EPS was found to have antibiofilm and antitumor [lung (A-549) and colon (Caco-2 and HT-29) cancer] activities.
33604750	3	57	theme	cancer	764:769	arg1	Caco-2					746:751	Caco-2	746:751	Caco-2	746:751	EPS was found to have antibiofilm and antitumor [lung (A-549) and colon (Caco-2 and HT-29) cancer] activities.
33604750	0	58	theme	antibiofilm	72:82	arg1	activities					84:93	antitumor and antibiofilm activities	58:93	activities	84:93	Exopolysaccharide of Anoxybacillus pushchinoensis G11 has antitumor and antibiofilm activities.
33604750	7	59	theme	molecular	1060:1068	arg1	weight					1070:1075	The molecular weight	1056:1075	The molecular weight of EPS	1056:1082	The molecular weight of EPS was determined as 75.5 kDa.
33604750	7	59	theme	molecular	1060:1068	arg1	75.5 kDa					1102:1109	75.5 kDa	1102:1109	75.5 kDa	1102:1109	The molecular weight of EPS was determined as 75.5 kDa.
33604750	11	60	theme	biological	1439:1448	arg1	activity					1450:1457	the biological activity	1435:1457	the biological activity of A. pushchinoensis	1435:1478	This is the first report on the EPS production potential and the biological activity of A. pushchinoensis.
33604750	1	61	theme	food	170:173	arg1	industries					194:203	the food and pharmaceutical industries	166:203	the food and pharmaceutical industries	166:203	Exopolysaccharides (EPS/EPSs) possess several various applications in the food and pharmaceutical industries.
34110171	2	0	theme	Ochroma	633:639	arg1	DOW					685:687	DOW	685:687	DOW	685:687	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	2	0	theme	Ochroma	633:639	arg1	microframe					673:682	Ochroma pyramidale wood-based cellulose microframe	633:682	Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation	633:723	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	4	1	theme	composite	1352:1360	arg1	material					1362:1369	The DOW-H3C7-4PA composite material	1335:1369	The DOW-H3C7-4PA composite material	1335:1369	The DOW-H3C7-4PA composite material also shows good shape stability and leakage resistance.
34110171	6	2	theme	energy	1843:1848	arg1	conversion					1850:1859	energy conversion	1843:1859	energy conversion	1843:1859	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	5	3	theme	energy	1568:1573	arg1	applications					1583:1594	photothermal energy storage applications	1555:1594	photothermal energy storage applications	1555:1594	In addition, the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA is considered to be a promising candidate for photothermal energy storage applications.
34110171	3	4	theme	coconut	759:765	arg1	oil					767:769	coconut oil	759:769	coconut oil	759:769	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	5	5	theme	high	1444:1447	arg1	candidate					1541:1549	a promising candidate	1529:1549	a promising candidate for photothermal energy storage applications	1529:1594	In addition, the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA is considered to be a promising candidate for photothermal energy storage applications.
34110171	5	5	theme	high	1444:1447	arg1	efficiency					1473:1482	the high photothermal conversion efficiency	1440:1482	the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA	1440:1507	In addition, the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA is considered to be a promising candidate for photothermal energy storage applications.
34110171	5	5	theme	high	1444:1447	arg1	%					1490:1490	65.71%	1485:1490	65.71%	1485:1490	In addition, the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA is considered to be a promising candidate for photothermal energy storage applications.
34110171	2	6	with	SPCMs	434:438	arg1	storage					487:493	phase change energy storage	467:493	phase change energy storage	467:493	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	2	6	with	SPCMs	434:438	arg1	photochromic					450:461	photochromic	450:461	photochromic	450:461	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	3	7	theme	radiation	1261:1269	arg1	cycles					1271:1276	UV radiation cycles	1258:1276	UV radiation cycles	1258:1276	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	3	8	theme	hexadecanol	744:754	arg1	ratio					735:739	the ratio	731:739	the ratio of hexadecanol to coconut oil	731:769	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	3	8	theme	hexadecanol	744:754	arg1	3:7					774:776	3:7	774:776	3:7	774:776	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	1	9	theme	composites	231:240	arg1	development					164:174	The development	160:174	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties,	160:358	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties, is essential for thermal energy storage.
34110171	1	9	theme	composites	231:240	arg1	essential					363:371	essential	363:371	essential	363:371	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties, is essential for thermal energy storage.
34110171	2	10	theme	wood-based	652:661	arg1	DOW					685:687	DOW	685:687	DOW	685:687	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	2	10	theme	wood-based	652:661	arg1	microframe					673:682	Ochroma pyramidale wood-based cellulose microframe	633:682	Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation	633:723	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	3	11	theme	temperature	1053:1063	arg1	adjustment					1065:1074	the environmental temperature adjustment	1035:1074	the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability	1035:1208	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	3	12	theme	phase	1283:1287	arg1	enthalpy					1300:1307	its phase transition enthalpy	1279:1307	its phase transition enthalpy	1279:1307	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	4	13	theme	leakage	1407:1413	arg1	resistance					1415:1424	leakage resistance	1407:1424	leakage resistance	1407:1424	The DOW-H3C7-4PA composite material also shows good shape stability and leakage resistance.
34110171	3	14	theme	thermal	1239:1245	arg1	cycles					1247:1252	100 thermal cycles	1235:1252	100 thermal cycles	1235:1252	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	3	15	theme	phosphotungstic	795:809	arg1	acid					811:814	phosphotungstic acid	795:814	phosphotungstic acid	795:814	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	3	16	dep	stability	1173:1181	arg1	addition					1139:1146	addition	1139:1146	addition	1139:1146	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	1	17	theme	phase	260:264	arg1	enthalpy					273:280	high phase change enthalpy	255:280	high phase change enthalpy	255:280	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties, is essential for thermal energy storage.
34110171	6	18	theme	cycle	1691:1695	arg1	reliability					1697:1707	good melting/freezing cycle reliability	1669:1707	good melting/freezing cycle reliability	1669:1707	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	4	19	theme	good	1382:1385	arg1	stability					1393:1401	good shape stability	1382:1401	good shape stability	1382:1401	The DOW-H3C7-4PA composite material also shows good shape stability and leakage resistance.
34110171	2	20	theme	phosphotungstic	602:616	arg1	PA					624:625	PA	624:625	PA	624:625	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	2	20	theme	phosphotungstic	602:616	arg1	acid					618:621	photochromic phosphotungstic acid	589:621	photochromic phosphotungstic acid (PA)	589:626	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	6	21	theme	good	1669:1672	arg1	reliability					1697:1707	good melting/freezing cycle reliability	1669:1707	good melting/freezing cycle reliability	1669:1707	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	3	22	theme	SPCM	856:859	arg1	material					871:878	the SPCM composite material	852:878	the SPCM composite material (DOW-H3C7-4PA)	852:893	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	3	22	theme	SPCM	856:859	arg1	DOW-H3C7-4PA					881:892	DOW-H3C7-4PA	881:892	DOW-H3C7-4PA	881:892	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	2	23	theme	composite	539:547	arg1	PCMs					549:552	organic binary composite PCMs	524:552	organic binary composite PCMs (hexadecanol/coconut oil, H/C)	524:583	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	0	24	theme	Wood-Based	67:76	arg1	Material					150:157	Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material	67:157	Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material	67:157	Design and Construction of Photochromic and Antileakage Reinforced Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material.
34110171	1	25	theme	thermal	377:383	arg1	storage					392:398	thermal energy storage	377:398	thermal energy storage	377:398	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties, is essential for thermal energy storage.
34110171	1	26	theme	shape-stable	196:207	arg1	material					222:229	high-performance shape-stable phase change material	179:229	high-performance shape-stable phase change material composites (SPCMs)	179:248	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties, is essential for thermal energy storage.
34110171	2	27	theme	organic	524:530	arg1	PCMs					549:552	organic binary composite PCMs	524:552	organic binary composite PCMs (hexadecanol/coconut oil, H/C)	524:583	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	6	28	theme	energy	1768:1773	arg1	ability					1786:1792	remarkable light-to-heat energy conversion ability	1743:1792	remarkable light-to-heat energy conversion ability	1743:1792	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	0	29	theme	Microframework/Hexadecanol-Coconut	88:121	arg1	Material					150:157	Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material	67:157	Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material	67:157	Design and Construction of Photochromic and Antileakage Reinforced Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material.
34110171	3	30	theme	areas	1096:1100	arg1	adjustment					1065:1074	the environmental temperature adjustment	1035:1074	the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability	1035:1208	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	6	31	theme	latent	1656:1661	arg1	heat					1663:1666	high latent heat	1651:1666	high latent heat	1651:1666	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	1	32	theme	change	215:220	arg1	material					222:229	high-performance shape-stable phase change material	179:229	high-performance shape-stable phase change material composites (SPCMs)	179:248	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties, is essential for thermal energy storage.
34110171	6	33	theme	remarkable	1743:1752	arg1	ability					1786:1792	remarkable light-to-heat energy conversion ability	1743:1792	remarkable light-to-heat energy conversion ability	1743:1792	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	0	34	theme	Composite	127:135	arg1	Material					150:157	Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material	67:157	Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material	67:157	Design and Construction of Photochromic and Antileakage Reinforced Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material.
34110171	3	35	theme	transition	919:928	arg1	enthalpy					930:937	a high phase transition enthalpy	906:937	a high phase transition enthalpy of 163.7 J/g	906:950	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	5	36	theme	conversion	1462:1471	arg1	candidate					1541:1549	a promising candidate	1529:1549	a promising candidate for photothermal energy storage applications	1529:1594	In addition, the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA is considered to be a promising candidate for photothermal energy storage applications.
34110171	5	36	theme	conversion	1462:1471	arg1	efficiency					1473:1482	the high photothermal conversion efficiency	1440:1482	the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA	1440:1507	In addition, the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA is considered to be a promising candidate for photothermal energy storage applications.
34110171	5	36	theme	conversion	1462:1471	arg1	%					1490:1490	65.71%	1485:1490	65.71%	1485:1490	In addition, the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA is considered to be a promising candidate for photothermal energy storage applications.
34110171	0	37	theme	Change	143:148	arg1	Material					150:157	Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material	67:157	Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material	67:157	Design and Construction of Photochromic and Antileakage Reinforced Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material.
34110171	3	38	theme	high	908:911	arg1	enthalpy					930:937	a high phase transition enthalpy	906:937	a high phase transition enthalpy of 163.7 J/g	906:950	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	2	39	theme	energy	480:485	arg1	storage					487:493	phase change energy storage	467:493	phase change energy storage	467:493	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	6	40	theme	photochromic	1715:1726	arg1	stability					1728:1736	high photochromic stability	1710:1736	high photochromic stability	1710:1736	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	3	41	theme	J/g	948:950	arg1	enthalpy					930:937	a high phase transition enthalpy	906:937	a high phase transition enthalpy of 163.7 J/g	906:950	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	3	41	theme	J/g	948:950	arg1	temperature					988:998	an appropriate phase transition temperature	956:998	an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability	956:1208	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	2	42	theme	phase	467:471	arg1	storage					487:493	phase change energy storage	467:493	phase change energy storage	467:493	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	6	43	theme	application	1818:1828	arg1	prospects					1830:1838	broad application prospects	1812:1838	broad application prospects	1812:1838	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	2	44	theme	simple	698:703	arg1	impregnation					712:723	simple vacuum impregnation	698:723	simple vacuum impregnation	698:723	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	1	45	theme	photochromic	335:346	arg1	properties					348:357	good photochromic properties	330:357	good photochromic properties	330:357	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties, is essential for thermal energy storage.
34110171	2	46	dep	PCMs	549:552	arg1	H/C					580:582	H/C	580:582	H/C	580:582	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	2	46	dep	PCMs	549:552	arg1	oil					575:577	hexadecanol/coconut oil	555:577	hexadecanol/coconut oil	555:577	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	0	47	theme	Photochromic	27:38	arg1	Construction					11:22	Construction	11:22	Construction	11:22	Design and Construction of Photochromic and Antileakage Reinforced Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material.
34110171	0	47	theme	Photochromic	27:38	arg1	Design					0:5	Design	0:5	Design	0:5	Design and Construction of Photochromic and Antileakage Reinforced Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material.
34110171	6	48	dep	them	1802:1805	arg1	show					1807:1810	show	1807:1810	show broad application prospects in energy conversion and storage devices	1807:1879	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	3	49	theme	phase	971:975	arg1	temperature					988:998	an appropriate phase transition temperature	956:998	an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability	956:1208	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	0	50	theme	Antileakage	44:54	arg1	Construction					11:22	Construction	11:22	Construction	11:22	Design and Construction of Photochromic and Antileakage Reinforced Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material.
34110171	0	50	theme	Antileakage	44:54	arg1	Design					0:5	Design	0:5	Design	0:5	Design and Construction of Photochromic and Antileakage Reinforced Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material.
34110171	1	51	theme	change	266:271	arg1	enthalpy					273:280	high phase change enthalpy	255:280	high phase change enthalpy	255:280	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties, is essential for thermal energy storage.
34110171	1	52	with	composites	231:240	arg1	efficiency					302:311	high conversion efficiency	286:311	high conversion efficiency	286:311	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties, is essential for thermal energy storage.
34110171	1	52	with	composites	231:240	arg1	properties					348:357	good photochromic properties	330:357	good photochromic properties	330:357	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties, is essential for thermal energy storage.
34110171	1	52	with	composites	231:240	arg1	enthalpy					273:280	high phase change enthalpy	255:280	high phase change enthalpy	255:280	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties, is essential for thermal energy storage.
34110171	6	53	contain	have	1646:1649	arg2	reliability					1697:1707	good melting/freezing cycle reliability	1669:1707	good melting/freezing cycle reliability	1669:1707	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	6	53	contain	have	1646:1649	arg1	DOW-H3C7-4PA					1632:1643	DOW-H3C7-4PA	1632:1643	DOW-H3C7-4PA	1632:1643	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	6	53	contain	have	1646:1649	arg2	ability					1786:1792	remarkable light-to-heat energy conversion ability	1743:1792	remarkable light-to-heat energy conversion ability	1743:1792	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	6	53	contain	have	1646:1649	arg2	heat					1663:1666	high latent heat	1651:1666	high latent heat	1651:1666	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	6	53	contain	have	1646:1649	arg1	SPCMs					1625:1629	the manufactured SPCMs	1608:1629	the manufactured SPCMs (DOW-H3C7-4PA)	1608:1644	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	6	53	contain	have	1646:1649	arg2	stability					1728:1736	high photochromic stability	1710:1736	high photochromic stability	1710:1736	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	6	54	theme	storage	1865:1871	arg1	devices					1873:1879	storage devices	1865:1879	storage devices	1865:1879	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	1	55	theme	conversion	291:300	arg1	efficiency					302:311	high conversion efficiency	286:311	high conversion efficiency	286:311	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties, is essential for thermal energy storage.
34110171	3	56	theme	excellent	1155:1163	arg1	stability					1173:1181	the excellent thermal stability	1151:1181	the excellent thermal stability	1151:1181	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	5	57	theme	photothermal	1555:1566	arg1	applications					1583:1594	photothermal energy storage applications	1555:1594	photothermal energy storage applications	1555:1594	In addition, the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA is considered to be a promising candidate for photothermal energy storage applications.
34110171	4	58	theme	DOW-H3C7-4PA	1339:1350	arg1	material					1362:1369	The DOW-H3C7-4PA composite material	1335:1369	The DOW-H3C7-4PA composite material	1335:1369	The DOW-H3C7-4PA composite material also shows good shape stability and leakage resistance.
34110171	5	59	theme	storage	1575:1581	arg1	applications					1583:1594	photothermal energy storage applications	1555:1594	photothermal energy storage applications	1555:1594	In addition, the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA is considered to be a promising candidate for photothermal energy storage applications.
34110171	5	60	theme	photothermal	1449:1460	arg1	candidate					1541:1549	a promising candidate	1529:1549	a promising candidate for photothermal energy storage applications	1529:1594	In addition, the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA is considered to be a promising candidate for photothermal energy storage applications.
34110171	5	60	theme	photothermal	1449:1460	arg1	efficiency					1473:1482	the high photothermal conversion efficiency	1440:1482	the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA	1440:1507	In addition, the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA is considered to be a promising candidate for photothermal energy storage applications.
34110171	5	60	theme	photothermal	1449:1460	arg1	%					1490:1490	65.71%	1485:1490	65.71%	1485:1490	In addition, the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA is considered to be a promising candidate for photothermal energy storage applications.
34110171	1	61	theme	material	222:229	arg1	SPCMs					243:247	SPCMs	243:247	SPCMs	243:247	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties, is essential for thermal energy storage.
34110171	1	61	theme	material	222:229	arg1	composites					231:240	high-performance shape-stable phase change material composites	179:240	high-performance shape-stable phase change material composites (SPCMs)	179:248	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties, is essential for thermal energy storage.
34110171	3	62	theme	photochromic	1187:1198	arg1	stability					1200:1208	photochromic stability	1187:1208	photochromic stability	1187:1208	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	2	63	theme	cellulose	663:671	arg1	DOW					685:687	DOW	685:687	DOW	685:687	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	2	63	theme	cellulose	663:671	arg1	microframe					673:682	Ochroma pyramidale wood-based cellulose microframe	633:682	Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation	633:723	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	3	64	theme	environmental	1039:1051	arg1	adjustment					1065:1074	the environmental temperature adjustment	1035:1074	the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability	1035:1208	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	2	65	theme	pyramidale	641:650	arg1	DOW					685:687	DOW	685:687	DOW	685:687	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	2	65	theme	pyramidale	641:650	arg1	microframe					673:682	Ochroma pyramidale wood-based cellulose microframe	633:682	Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation	633:723	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	3	66	theme	UV	1258:1259	arg1	cycles					1271:1276	UV radiation cycles	1258:1276	UV radiation cycles	1258:1276	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	4	67	theme	shape	1387:1391	arg1	stability					1393:1401	good shape stability	1382:1401	good shape stability	1382:1401	The DOW-H3C7-4PA composite material also shows good shape stability and leakage resistance.
34110171	6	68	theme	high	1710:1713	arg1	stability					1728:1736	high photochromic stability	1710:1736	high photochromic stability	1710:1736	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	3	69	theme	transition	1289:1298	arg1	enthalpy					1300:1307	its phase transition enthalpy	1279:1307	its phase transition enthalpy	1279:1307	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	1	70	theme	high	255:258	arg1	enthalpy					273:280	high phase change enthalpy	255:280	high phase change enthalpy	255:280	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties, is essential for thermal energy storage.
34110171	3	71	theme	acid	811:814	arg1	ratio					735:739	the ratio	731:739	the ratio of hexadecanol to coconut oil	731:769	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	3	71	theme	acid	811:814	arg1	3:7					774:776	3:7	774:776	3:7	774:776	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	3	71	theme	acid	811:814	arg1	ratio					786:790	the ratio	782:790	the ratio of phosphotungstic acid to N	782:819	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	6	72	theme	melting/freezing	1674:1689	arg1	reliability					1697:1707	good melting/freezing cycle reliability	1669:1707	good melting/freezing cycle reliability	1669:1707	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	2	73	theme	photochromic	589:600	arg1	PA					624:625	PA	624:625	PA	624:625	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	2	73	theme	photochromic	589:600	arg1	acid					618:621	photochromic phosphotungstic acid	589:621	photochromic phosphotungstic acid (PA)	589:626	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	0	74	theme	Cellulose	78:86	arg1	Material					150:157	Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material	67:157	Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material	67:157	Design and Construction of Photochromic and Antileakage Reinforced Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material.
34110171	1	75	theme	high-performance	179:194	arg1	material					222:229	high-performance shape-stable phase change material	179:229	high-performance shape-stable phase change material composites (SPCMs)	179:248	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties, is essential for thermal energy storage.
34110171	3	76	theme	composite	861:869	arg1	material					871:878	the SPCM composite material	852:878	the SPCM composite material (DOW-H3C7-4PA)	852:893	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	3	76	theme	composite	861:869	arg1	DOW-H3C7-4PA					881:892	DOW-H3C7-4PA	881:892	DOW-H3C7-4PA	881:892	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	2	77	theme	binary	532:537	arg1	PCMs					549:552	organic binary composite PCMs	524:552	organic binary composite PCMs (hexadecanol/coconut oil, H/C)	524:583	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	6	78	theme	conversion	1775:1784	arg1	ability					1786:1792	remarkable light-to-heat energy conversion ability	1743:1792	remarkable light-to-heat energy conversion ability	1743:1792	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	0	79	theme	Oil	123:125	arg1	Material					150:157	Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material	67:157	Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material	67:157	Design and Construction of Photochromic and Antileakage Reinforced Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material.
34110171	1	80	theme	energy	385:390	arg1	storage					392:398	thermal energy storage	377:398	thermal energy storage	377:398	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties, is essential for thermal energy storage.
34110171	3	81	theme	high-temperature	1079:1094	arg1	areas					1096:1100	high-temperature areas	1079:1100	high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability	1079:1208	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	3	81	theme	high-temperature	1079:1094	arg1	°C					1107:1108	>40 °C	1103:1108	>40 °C	1103:1108	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	1	82	theme	phase	209:213	arg1	material					222:229	high-performance shape-stable phase change material	179:229	high-performance shape-stable phase change material composites (SPCMs)	179:248	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties, is essential for thermal energy storage.
34110171	6	83	theme	light-to-heat	1754:1766	arg1	ability					1786:1792	remarkable light-to-heat energy conversion ability	1743:1792	remarkable light-to-heat energy conversion ability	1743:1792	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	0	84	theme	Phase	137:141	arg1	Material					150:157	Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material	67:157	Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material	67:157	Design and Construction of Photochromic and Antileakage Reinforced Wood-Based Cellulose Microframework/Hexadecanol-Coconut Oil Composite Phase Change Material.
34110171	6	85	theme	high	1651:1654	arg1	heat					1663:1666	high latent heat	1651:1666	high latent heat	1651:1666	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	2	86	theme	hexadecanol/coconut	555:573	arg1	H/C					580:582	H/C	580:582	H/C	580:582	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	2	86	theme	hexadecanol/coconut	555:573	arg1	oil					575:577	hexadecanol/coconut oil	555:577	hexadecanol/coconut oil	555:577	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	5	87	theme	DOW-H3C7-4PA	1496:1507	arg1	candidate					1541:1549	a promising candidate	1529:1549	a promising candidate for photothermal energy storage applications	1529:1594	In addition, the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA is considered to be a promising candidate for photothermal energy storage applications.
34110171	5	87	theme	DOW-H3C7-4PA	1496:1507	arg1	efficiency					1473:1482	the high photothermal conversion efficiency	1440:1482	the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA	1440:1507	In addition, the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA is considered to be a promising candidate for photothermal energy storage applications.
34110171	5	87	theme	DOW-H3C7-4PA	1496:1507	arg1	%					1490:1490	65.71%	1485:1490	65.71%	1485:1490	In addition, the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA is considered to be a promising candidate for photothermal energy storage applications.
34110171	3	88	theme	phase	913:917	arg1	enthalpy					930:937	a high phase transition enthalpy	906:937	a high phase transition enthalpy of 163.7 J/g	906:950	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	2	89	theme	change	473:478	arg1	storage					487:493	phase change energy storage	467:493	phase change energy storage	467:493	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	2	90	theme	vacuum	705:710	arg1	impregnation					712:723	simple vacuum impregnation	698:723	simple vacuum impregnation	698:723	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	1	91	theme	good	330:333	arg1	properties					348:357	good photochromic properties	330:357	good photochromic properties	330:357	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties, is essential for thermal energy storage.
34110171	6	92	theme	manufactured	1612:1623	arg1	DOW-H3C7-4PA					1632:1643	DOW-H3C7-4PA	1632:1643	DOW-H3C7-4PA	1632:1643	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	6	92	theme	manufactured	1612:1623	arg1	SPCMs					1625:1629	the manufactured SPCMs	1608:1629	the manufactured SPCMs (DOW-H3C7-4PA)	1608:1644	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	6	93	theme	broad	1812:1816	arg1	application					1818:1828	broad application	1812:1828	broad application prospects	1812:1838	Therefore, the manufactured SPCMs (DOW-H3C7-4PA) have high latent heat, good melting/freezing cycle reliability, high photochromic stability, and remarkable light-to-heat energy conversion ability, making them show broad application prospects in energy conversion and storage devices.
34110171	5	94	theme	promising	1531:1539	arg1	candidate					1541:1549	a promising candidate	1529:1549	a promising candidate for photothermal energy storage applications	1529:1594	In addition, the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA is considered to be a promising candidate for photothermal energy storage applications.
34110171	5	94	theme	promising	1531:1539	arg1	efficiency					1473:1482	the high photothermal conversion efficiency	1440:1482	the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA	1440:1507	In addition, the high photothermal conversion efficiency (65.71%) of DOW-H3C7-4PA is considered to be a promising candidate for photothermal energy storage applications.
34110171	2	95	theme	SPCMs	434:438	arg1	type					426:429	one type	422:429	one type of SPCMs with both photochromic and phase change energy storage	422:493	Here, we report that one type of SPCMs with both photochromic and phase change energy storage is obtained by incorporating organic binary composite PCMs (hexadecanol/coconut oil, H/C) and photochromic phosphotungstic acid (PA) into Ochroma pyramidale wood-based cellulose microframe (DOW) through simple vacuum impregnation.
34110171	3	96	theme	transition	977:986	arg1	temperature					988:998	an appropriate phase transition temperature	956:998	an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability	956:1208	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	3	97	theme	appropriate	959:969	arg1	temperature					988:998	an appropriate phase transition temperature	956:998	an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability	956:1208	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	3	98	theme	thermal	1165:1171	arg1	stability					1173:1181	the excellent thermal stability	1151:1181	the excellent thermal stability	1151:1181	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	3	99	theme	°C	1009:1010	arg1	enthalpy					930:937	a high phase transition enthalpy	906:937	a high phase transition enthalpy of 163.7 J/g	906:950	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	3	99	theme	°C	1009:1010	arg1	temperature					988:998	an appropriate phase transition temperature	956:998	an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability	956:1208	When the ratio of hexadecanol to coconut oil is 3:7 and the ratio of phosphotungstic acid to N,N-dimethylacetamide is 4:13.6, the SPCM composite material (DOW-H3C7-4PA) represents a high phase transition enthalpy of 163.7 J/g and an appropriate phase transition temperature of 42.55 °C that can be applied to the environmental temperature adjustment of high-temperature areas (>40 °C) mentioned in this paper, in addition to the excellent thermal stability and photochromic stability; for example, even after 100 thermal cycles and UV radiation cycles, its phase transition enthalpy remains almost unchanged.
34110171	1	100	theme	high	286:289	arg1	efficiency					302:311	high conversion efficiency	286:311	high conversion efficiency	286:311	The development of high-performance shape-stable phase change material composites (SPCMs) with high phase change enthalpy and high conversion efficiency, especially with good photochromic properties, is essential for thermal energy storage.
34134416	0	0	theme	chemical	65:72	arg1	coupling					74:81	interfacial chemical coupling	53:81	interfacial chemical coupling	53:81	2D-layered Mg(OH)2 material adsorbing cellobiose via interfacial chemical coupling and its applications in handling toxic Cd2+ and UO22+ ions.
34134416	3	1	theme	different	743:751	arg1	layers					753:758	different layers	743:758	different layers	743:758	In this regard, density functional theory first-principle calculations associated with experimental study have synergistically examined two-dimensional (2D) magnesium hydroxide material with different layers and their adsorption toward cellobiose.
34134416	0	2	theme	interfacial	53:63	arg1	coupling					74:81	interfacial chemical coupling	53:81	interfacial chemical coupling	53:81	2D-layered Mg(OH)2 material adsorbing cellobiose via interfacial chemical coupling and its applications in handling toxic Cd2+ and UO22+ ions.
34134416	3	3	theme	hydroxide	719:727	arg1	material					729:736	two-dimensional (2D) magnesium hydroxide material	688:736	two-dimensional (2D) magnesium hydroxide material	688:736	In this regard, density functional theory first-principle calculations associated with experimental study have synergistically examined two-dimensional (2D) magnesium hydroxide material with different layers and their adsorption toward cellobiose.
34134416	4	4	theme	dispersion	900:909	arg1	correction					911:920	the dispersion correction	896:920	the dispersion correction	896:920	Hydrogen bonds are found responsible for the interfacial coupling, which make it vital to cover the dispersion correction in the calculation.
34134416	5	5	theme	average	946:952	arg1	energy					965:970	The average adsorption energy	942:970	The average adsorption energy	942:970	The average adsorption energy ranges from -0.29 to -0.35 eV, falling well within the range of reported hydrogen-bonding strength.
34134416	1	6	theme	structure-property	331:348	arg1	relationship					350:361	structure-property relationship	331:361	structure-property relationship	331:361	The interfacial chemistry of nanocomposite materials is of overarching importance in the separation and purification science; moreover, its understanding helps to guide synthesis, clarify structure-property relationship and unearth novel applications.
34134416	8	7	theme	uranium	1475:1481	arg1	extraction					1483:1492	uranium extraction	1475:1492	uranium extraction	1475:1492	The ion-exchanging mechanism was proposed for cadmium removal and the outer-sphere adsorption one for uranium extraction.
34134416	6	8	theme	toxic	1233:1237	arg1	ion					1244:1246	toxic Cd2+ ion	1233:1246	toxic Cd2+ ion	1233:1246	On the basis of calculated structural/interfacial properties and experimental findings, the 2D Mg(OH)2 in terms of three-layer model was unraveled to substitute toxic Cd2+ ion and sorb radioactive UO22+ that is coordinated by water and hydroxyl groups.
34134416	6	9	theme	model	1199:1203	arg1	terms					1178:1182	terms	1178:1182	terms of three-layer model	1178:1203	On the basis of calculated structural/interfacial properties and experimental findings, the 2D Mg(OH)2 in terms of three-layer model was unraveled to substitute toxic Cd2+ ion and sorb radioactive UO22+ that is coordinated by water and hydroxyl groups.
34134416	6	10	theme	hydroxyl	1308:1315	arg1	groups					1317:1322	hydroxyl groups	1308:1322	hydroxyl groups	1308:1322	On the basis of calculated structural/interfacial properties and experimental findings, the 2D Mg(OH)2 in terms of three-layer model was unraveled to substitute toxic Cd2+ ion and sorb radioactive UO22+ that is coordinated by water and hydroxyl groups.
34134416	8	11	theme	cadmium	1419:1425	arg1	removal					1427:1433	cadmium removal	1419:1433	cadmium removal	1419:1433	The ion-exchanging mechanism was proposed for cadmium removal and the outer-sphere adsorption one for uranium extraction.
34134416	1	12	theme	unearth	367:373	arg1	applications					381:392	unearth novel applications	367:392	unearth novel applications	367:392	The interfacial chemistry of nanocomposite materials is of overarching importance in the separation and purification science; moreover, its understanding helps to guide synthesis, clarify structure-property relationship and unearth novel applications.
34134416	6	13	dep	properties	1122:1131	arg1	the					1075:1077	the	1075:1077	the	1075:1077	On the basis of calculated structural/interfacial properties and experimental findings, the 2D Mg(OH)2 in terms of three-layer model was unraveled to substitute toxic Cd2+ ion and sorb radioactive UO22+ that is coordinated by water and hydroxyl groups.
34134416	6	13	dep	properties	1122:1131	arg1	basis					1079:1083	basis	1079:1083	basis	1079:1083	On the basis of calculated structural/interfacial properties and experimental findings, the 2D Mg(OH)2 in terms of three-layer model was unraveled to substitute toxic Cd2+ ion and sorb radioactive UO22+ that is coordinated by water and hydroxyl groups.
34134416	1	14	theme	interfacial	147:157	arg1	chemistry					159:167	The interfacial chemistry	143:167	The interfacial chemistry of nanocomposite materials	143:194	The interfacial chemistry of nanocomposite materials is of overarching importance in the separation and purification science; moreover, its understanding helps to guide synthesis, clarify structure-property relationship and unearth novel applications.
34134416	0	15	theme	Mg	11:12	arg1	material					19:26	2D-layered Mg(OH)2 material	0:26	2D-layered Mg(OH)2 material	0:26	2D-layered Mg(OH)2 material adsorbing cellobiose via interfacial chemical coupling and its applications in handling toxic Cd2+ and UO22+ ions.
34134416	1	16	theme	novel	375:379	arg1	applications					381:392	unearth novel applications	367:392	unearth novel applications	367:392	The interfacial chemistry of nanocomposite materials is of overarching importance in the separation and purification science; moreover, its understanding helps to guide synthesis, clarify structure-property relationship and unearth novel applications.
34134416	0	17	theme	2D-layered	0:9	arg1	material					19:26	2D-layered Mg(OH)2 material	0:26	2D-layered Mg(OH)2 material	0:26	2D-layered Mg(OH)2 material adsorbing cellobiose via interfacial chemical coupling and its applications in handling toxic Cd2+ and UO22+ ions.
34134416	3	18	theme	density	568:574	arg1	calculations					610:621	density functional theory first-principle calculations	568:621	density functional theory first-principle calculations associated with experimental study	568:656	In this regard, density functional theory first-principle calculations associated with experimental study have synergistically examined two-dimensional (2D) magnesium hydroxide material with different layers and their adsorption toward cellobiose.
34134416	6	19	theme	experimental	1137:1148	arg1	findings					1150:1157	experimental findings	1137:1157	experimental findings	1137:1157	On the basis of calculated structural/interfacial properties and experimental findings, the 2D Mg(OH)2 in terms of three-layer model was unraveled to substitute toxic Cd2+ ion and sorb radioactive UO22+ that is coordinated by water and hydroxyl groups.
34134416	0	20	theme	OH	14:15	arg1	material					19:26	2D-layered Mg(OH)2 material	0:26	2D-layered Mg(OH)2 material	0:26	2D-layered Mg(OH)2 material adsorbing cellobiose via interfacial chemical coupling and its applications in handling toxic Cd2+ and UO22+ ions.
34134416	0	21	theme	handling	107:114	arg1	Cd2+					122:125	handling toxic Cd2+ and UO22+ ions	107:140	Cd2+	122:125	2D-layered Mg(OH)2 material adsorbing cellobiose via interfacial chemical coupling and its applications in handling toxic Cd2+ and UO22+ ions.
34134416	6	22	theme	radioactive	1257:1267	arg1	UO22+					1269:1273	sorb radioactive UO22+	1252:1273	sorb radioactive UO22+	1252:1273	On the basis of calculated structural/interfacial properties and experimental findings, the 2D Mg(OH)2 in terms of three-layer model was unraveled to substitute toxic Cd2+ ion and sorb radioactive UO22+ that is coordinated by water and hydroxyl groups.
34134416	4	23	theme	Hydrogen	800:807	arg1	bonds					809:813	Hydrogen bonds	800:813	Hydrogen bonds	800:813	Hydrogen bonds are found responsible for the interfacial coupling, which make it vital to cover the dispersion correction in the calculation.
34134416	3	24	theme	first-principle	594:608	arg1	calculations					610:621	density functional theory first-principle calculations	568:621	density functional theory first-principle calculations associated with experimental study	568:656	In this regard, density functional theory first-principle calculations associated with experimental study have synergistically examined two-dimensional (2D) magnesium hydroxide material with different layers and their adsorption toward cellobiose.
34134416	1	25	theme	nanocomposite	172:184	arg1	materials					186:194	nanocomposite materials	172:194	nanocomposite materials	172:194	The interfacial chemistry of nanocomposite materials is of overarching importance in the separation and purification science; moreover, its understanding helps to guide synthesis, clarify structure-property relationship and unearth novel applications.
34134416	2	26	theme	complicated	434:444	arg1	structures					452:461	rather complicated local structures	427:461	rather complicated local structures	427:461	However, the composites feature rather complicated local structures and hydrogen bonds are often involved in the interface and the vicinity of active sites.
34134416	6	27	theme	2D	1164:1165	arg1	OH					1170:1171	OH	1170:1171	OH	1170:1171	On the basis of calculated structural/interfacial properties and experimental findings, the 2D Mg(OH)2 in terms of three-layer model was unraveled to substitute toxic Cd2+ ion and sorb radioactive UO22+ that is coordinated by water and hydroxyl groups.
34134416	6	27	theme	2D	1164:1165	arg1	Mg					1167:1168	the 2D Mg(OH)2	1160:1173	the 2D Mg(OH)2 in terms of three-layer model	1160:1203	On the basis of calculated structural/interfacial properties and experimental findings, the 2D Mg(OH)2 in terms of three-layer model was unraveled to substitute toxic Cd2+ ion and sorb radioactive UO22+ that is coordinated by water and hydroxyl groups.
34134416	1	28	theme	materials	186:194	arg1	chemistry					159:167	The interfacial chemistry	143:167	The interfacial chemistry of nanocomposite materials	143:194	The interfacial chemistry of nanocomposite materials is of overarching importance in the separation and purification science; moreover, its understanding helps to guide synthesis, clarify structure-property relationship and unearth novel applications.
34134416	8	29	theme	ion-exchanging	1377:1390	arg1	mechanism					1392:1400	The ion-exchanging mechanism	1373:1400	The ion-exchanging mechanism	1373:1400	The ion-exchanging mechanism was proposed for cadmium removal and the outer-sphere adsorption one for uranium extraction.
34134416	2	30	theme	sites	545:549	arg1	interface					508:516	the interface	504:516	the interface	504:516	However, the composites feature rather complicated local structures and hydrogen bonds are often involved in the interface and the vicinity of active sites.
34134416	2	30	theme	sites	545:549	arg1	vicinity					526:533	the vicinity	522:533	the vicinity of active sites	522:549	However, the composites feature rather complicated local structures and hydrogen bonds are often involved in the interface and the vicinity of active sites.
34134416	3	31	theme	two-dimensional	688:702	arg1	material					729:736	two-dimensional (2D) magnesium hydroxide material	688:736	two-dimensional (2D) magnesium hydroxide material	688:736	In this regard, density functional theory first-principle calculations associated with experimental study have synergistically examined two-dimensional (2D) magnesium hydroxide material with different layers and their adsorption toward cellobiose.
34134416	2	32	dep	composites	408:417	arg1	feature					419:425	feature	419:425	feature	419:425	However, the composites feature rather complicated local structures and hydrogen bonds are often involved in the interface and the vicinity of active sites.
34134416	6	33	theme	structural/interfacial	1099:1120	arg1	properties					1122:1131	calculated structural/interfacial properties	1088:1131	calculated structural/interfacial properties	1088:1131	On the basis of calculated structural/interfacial properties and experimental findings, the 2D Mg(OH)2 in terms of three-layer model was unraveled to substitute toxic Cd2+ ion and sorb radioactive UO22+ that is coordinated by water and hydroxyl groups.
34134416	0	34	theme	toxic	116:120	arg1	Cd2+					122:125	handling toxic Cd2+ and UO22+ ions	107:140	Cd2+	122:125	2D-layered Mg(OH)2 material adsorbing cellobiose via interfacial chemical coupling and its applications in handling toxic Cd2+ and UO22+ ions.
34134416	2	35	theme	local	446:450	arg1	structures					452:461	rather complicated local structures	427:461	rather complicated local structures	427:461	However, the composites feature rather complicated local structures and hydrogen bonds are often involved in the interface and the vicinity of active sites.
34134416	2	36	theme	active	538:543	arg1	sites					545:549	active sites	538:549	active sites	538:549	However, the composites feature rather complicated local structures and hydrogen bonds are often involved in the interface and the vicinity of active sites.
34134416	6	37	theme	calculated	1088:1097	arg1	properties					1122:1131	calculated structural/interfacial properties	1088:1131	calculated structural/interfacial properties	1088:1131	On the basis of calculated structural/interfacial properties and experimental findings, the 2D Mg(OH)2 in terms of three-layer model was unraveled to substitute toxic Cd2+ ion and sorb radioactive UO22+ that is coordinated by water and hydroxyl groups.
34134416	0	38	theme	UO22+	131:135	arg1	ions					137:140	handling toxic Cd2+ and UO22+ ions	107:140	ions	137:140	2D-layered Mg(OH)2 material adsorbing cellobiose via interfacial chemical coupling and its applications in handling toxic Cd2+ and UO22+ ions.
34134416	3	39	theme	functional	576:585	arg1	calculations					610:621	density functional theory first-principle calculations	568:621	density functional theory first-principle calculations associated with experimental study	568:656	In this regard, density functional theory first-principle calculations associated with experimental study have synergistically examined two-dimensional (2D) magnesium hydroxide material with different layers and their adsorption toward cellobiose.
34134416	5	40	theme	adsorption	954:963	arg1	energy					965:970	The average adsorption energy	942:970	The average adsorption energy	942:970	The average adsorption energy ranges from -0.29 to -0.35 eV, falling well within the range of reported hydrogen-bonding strength.
34134416	2	41	theme	hydrogen	467:474	arg1	bonds					476:480	hydrogen bonds	467:480	hydrogen bonds	467:480	However, the composites feature rather complicated local structures and hydrogen bonds are often involved in the interface and the vicinity of active sites.
34134416	0	42	from	applications	91:102	arg1	Cd2+					122:125	handling toxic Cd2+ and UO22+ ions	107:140	Cd2+	122:125	2D-layered Mg(OH)2 material adsorbing cellobiose via interfacial chemical coupling and its applications in handling toxic Cd2+ and UO22+ ions.
34134416	0	42	from	applications	91:102	arg1	ions					137:140	handling toxic Cd2+ and UO22+ ions	107:140	ions	137:140	2D-layered Mg(OH)2 material adsorbing cellobiose via interfacial chemical coupling and its applications in handling toxic Cd2+ and UO22+ ions.
34134416	3	43	theme	theory	587:592	arg1	calculations					610:621	density functional theory first-principle calculations	568:621	density functional theory first-principle calculations associated with experimental study	568:656	In this regard, density functional theory first-principle calculations associated with experimental study have synergistically examined two-dimensional (2D) magnesium hydroxide material with different layers and their adsorption toward cellobiose.
34134416	6	44	theme	Cd2+	1239:1242	arg1	ion					1244:1246	toxic Cd2+ ion	1233:1246	toxic Cd2+ ion	1233:1246	On the basis of calculated structural/interfacial properties and experimental findings, the 2D Mg(OH)2 in terms of three-layer model was unraveled to substitute toxic Cd2+ ion and sorb radioactive UO22+ that is coordinated by water and hydroxyl groups.
34134416	3	45	theme	magnesium	709:717	arg1	material					729:736	two-dimensional (2D) magnesium hydroxide material	688:736	two-dimensional (2D) magnesium hydroxide material	688:736	In this regard, density functional theory first-principle calculations associated with experimental study have synergistically examined two-dimensional (2D) magnesium hydroxide material with different layers and their adsorption toward cellobiose.
34134416	3	46	theme	experimental	639:650	arg1	study					652:656	experimental study	639:656	experimental study	639:656	In this regard, density functional theory first-principle calculations associated with experimental study have synergistically examined two-dimensional (2D) magnesium hydroxide material with different layers and their adsorption toward cellobiose.
34134416	6	47	theme	three-layer	1187:1197	arg1	model					1199:1203	three-layer model	1187:1203	three-layer model	1187:1203	On the basis of calculated structural/interfacial properties and experimental findings, the 2D Mg(OH)2 in terms of three-layer model was unraveled to substitute toxic Cd2+ ion and sorb radioactive UO22+ that is coordinated by water and hydroxyl groups.
34134416	1	48	theme	overarching	202:212	arg1	importance					214:223	overarching importance	202:223	overarching importance	202:223	The interfacial chemistry of nanocomposite materials is of overarching importance in the separation and purification science; moreover, its understanding helps to guide synthesis, clarify structure-property relationship and unearth novel applications.
34134416	1	49	from	importance	214:223	arg1	science					260:266	science	260:266	science	260:266	The interfacial chemistry of nanocomposite materials is of overarching importance in the separation and purification science; moreover, its understanding helps to guide synthesis, clarify structure-property relationship and unearth novel applications.
34134416	1	50	dep	separation	232:241	arg1	the					228:230	the	228:230	the	228:230	The interfacial chemistry of nanocomposite materials is of overarching importance in the separation and purification science; moreover, its understanding helps to guide synthesis, clarify structure-property relationship and unearth novel applications.
34134416	5	51	theme	hydrogen-bonding	1045:1060	arg1	strength					1062:1069	reported hydrogen-bonding strength	1036:1069	reported hydrogen-bonding strength	1036:1069	The average adsorption energy ranges from -0.29 to -0.35 eV, falling well within the range of reported hydrogen-bonding strength.
34134416	6	52	from	Mg	1167:1168	arg1	terms					1178:1182	terms	1178:1182	terms of three-layer model	1178:1203	On the basis of calculated structural/interfacial properties and experimental findings, the 2D Mg(OH)2 in terms of three-layer model was unraveled to substitute toxic Cd2+ ion and sorb radioactive UO22+ that is coordinated by water and hydroxyl groups.
34134416	5	53	theme	from -0.29	979:988	arg1	to -0.35 eV					990:1000	from -0.29 to -0.35 eV	979:1000	from -0.29 to -0.35 eV	979:1000	The average adsorption energy ranges from -0.29 to -0.35 eV, falling well within the range of reported hydrogen-bonding strength.
34134416	0	54	from	coupling	74:81	arg1	Cd2+					122:125	handling toxic Cd2+ and UO22+ ions	107:140	Cd2+	122:125	2D-layered Mg(OH)2 material adsorbing cellobiose via interfacial chemical coupling and its applications in handling toxic Cd2+ and UO22+ ions.
34134416	0	54	from	coupling	74:81	arg1	ions					137:140	handling toxic Cd2+ and UO22+ ions	107:140	ions	137:140	2D-layered Mg(OH)2 material adsorbing cellobiose via interfacial chemical coupling and its applications in handling toxic Cd2+ and UO22+ ions.
34134416	5	55	theme	strength	1062:1069	arg1	range					1027:1031	the range	1023:1031	the range of reported hydrogen-bonding strength	1023:1069	The average adsorption energy ranges from -0.29 to -0.35 eV, falling well within the range of reported hydrogen-bonding strength.
34134416	8	56	theme	outer-sphere	1443:1454	arg1	adsorption					1456:1465	the outer-sphere adsorption one	1439:1469	the outer-sphere adsorption one	1439:1469	The ion-exchanging mechanism was proposed for cadmium removal and the outer-sphere adsorption one for uranium extraction.
34134416	5	57	theme	reported	1036:1043	arg1	strength					1062:1069	reported hydrogen-bonding strength	1036:1069	reported hydrogen-bonding strength	1036:1069	The average adsorption energy ranges from -0.29 to -0.35 eV, falling well within the range of reported hydrogen-bonding strength.
34134416	4	58	theme	interfacial	845:855	arg1	coupling					857:864	the interfacial coupling	841:864	the interfacial coupling	841:864	Hydrogen bonds are found responsible for the interfacial coupling, which make it vital to cover the dispersion correction in the calculation.
34134416	6	59	theme	sorb	1252:1255	arg1	UO22+					1269:1273	sorb radioactive UO22+	1252:1273	sorb radioactive UO22+	1252:1273	On the basis of calculated structural/interfacial properties and experimental findings, the 2D Mg(OH)2 in terms of three-layer model was unraveled to substitute toxic Cd2+ ion and sorb radioactive UO22+ that is coordinated by water and hydroxyl groups.
34134416	3	60	dep	two-dimensional	688:702	arg1	2D					705:706	2D	705:706	2D	705:706	In this regard, density functional theory first-principle calculations associated with experimental study have synergistically examined two-dimensional (2D) magnesium hydroxide material with different layers and their adsorption toward cellobiose.
34889171	8	0	theme	±	943:943	arg1	HMF					964:966	28.45 ± 1.16 μmol l-1 free HMF	937:966	28.45 ± 1.16 μmol l-1 free HMF	937:966	Texture parameters were higher (1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess) when compared to the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess).
34889171	6	1	theme	5-Hydroxymethylfurfural	671:693	arg1	concentration					649:661	a higher concentration	640:661	a higher concentration of free 5-Hydroxymethylfurfural (HMF) (133.77 ± 3.42 μmol l-1)	640:724	The main results show that the homogenized lactose-free DL obtained a higher concentration of free 5-Hydroxymethylfurfural (HMF) (133.77 ± 3.42 μmol l-1).
34889171	6	2	theme	main	576:579	arg1	results					581:587	The main results	572:587	The main results	572:587	The main results show that the homogenized lactose-free DL obtained a higher concentration of free 5-Hydroxymethylfurfural (HMF) (133.77 ± 3.42 μmol l-1).
34889171	0	3	theme	texture	74:80	arg1	profile					82:88	texture profile	74:88	texture profile	74:88	Lactose-free Dulce de leche: compositional characterization, browning and texture profile.
34889171	8	4	theme	Texture	793:799	arg1	parameters					801:810	Texture parameters	793:810	Texture parameters	793:810	Texture parameters were higher (1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess) when compared to the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess).
34889171	4	5	theme	different	432:440	arg1	treatments					442:451	Four different treatments	427:451	Four different treatments	427:451	Four different treatments were studied.
34889171	8	6	theme	μmol	950:953	arg1	HMF					964:966	28.45 ± 1.16 μmol l-1 free HMF	937:966	28.45 ± 1.16 μmol l-1 free HMF	937:966	Texture parameters were higher (1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess) when compared to the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess).
34889171	9	7	theme	high	1059:1062	arg1	demand					1064:1069	high demand	1059:1069	high demand	1059:1069	Lactose-free products are in high demand by consumers; however, the results of this work highlight the challenges to properly control the browning and the texture parameters of DL.
34889171	9	8	theme	texture	1185:1191	arg1	parameters					1193:1202	the texture parameters	1181:1202	the texture parameters	1181:1202	Lactose-free products are in high demand by consumers; however, the results of this work highlight the challenges to properly control the browning and the texture parameters of DL.
34889171	5	9	theme	physico-chemical	538:553	arg1	characteristics					555:569	physico-chemical characteristics	538:569	physico-chemical characteristics	538:569	The final products were evaluated in relation to their composition and physico-chemical characteristics.
34889171	2	10	theme	Fluid	249:253	arg1	milk					255:258	Fluid milk	249:258	Fluid milk used	249:263	Fluid milk used was divided into two portions and, in one of them, enzymatic hydrolysis of lactose was carried through.
34889171	6	11	theme	free	666:669	arg1	HMF					696:698	HMF	696:698	HMF	696:698	The main results show that the homogenized lactose-free DL obtained a higher concentration of free 5-Hydroxymethylfurfural (HMF) (133.77 ± 3.42 μmol l-1).
34889171	6	11	theme	free	666:669	arg1	5-Hydroxymethylfurfural					671:693	free 5-Hydroxymethylfurfural	666:693	free 5-Hydroxymethylfurfural (HMF) (133.77 ± 3.42 μmol l-1)	666:724	The main results show that the homogenized lactose-free DL obtained a higher concentration of free 5-Hydroxymethylfurfural (HMF) (133.77 ± 3.42 μmol l-1).
34889171	6	11	theme	free	666:669	arg1	l-1					721:723	133.77 ± 3.42 μmol l-1	702:723	133.77 ± 3.42 μmol l-1	702:723	The main results show that the homogenized lactose-free DL obtained a higher concentration of free 5-Hydroxymethylfurfural (HMF) (133.77 ± 3.42 μmol l-1).
34889171	8	12	theme	free	959:962	arg1	HMF					964:966	28.45 ± 1.16 μmol l-1 free HMF	937:966	28.45 ± 1.16 μmol l-1 free HMF	937:966	Texture parameters were higher (1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess) when compared to the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess).
34889171	8	13	dep	product	928:934	arg1	gumminess					1018:1026	406.20 ± 311.69 g gumminess	1000:1026	406.20 ± 311.69 g gumminess	1000:1026	Texture parameters were higher (1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess) when compared to the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess).
34889171	8	13	dep	product	928:934	arg1	HMF					964:966	28.45 ± 1.16 μmol l-1 free HMF	937:966	28.45 ± 1.16 μmol l-1 free HMF	937:966	Texture parameters were higher (1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess) when compared to the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess).
34889171	8	13	dep	product	928:934	arg1	hardness					986:993	437.17 ± 279.3 g hardness	969:993	437.17 ± 279.3 g hardness	969:993	Texture parameters were higher (1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess) when compared to the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess).
34889171	8	14	theme	l-1	955:957	arg1	HMF					964:966	28.45 ± 1.16 μmol l-1 free HMF	937:966	28.45 ± 1.16 μmol l-1 free HMF	937:966	Texture parameters were higher (1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess) when compared to the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess).
34889171	8	15	theme	traditional	916:926	arg1	product					928:934	the homogenized traditional product	900:934	the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess)	900:1027	Texture parameters were higher (1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess) when compared to the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess).
34889171	7	16	theme	Maillard	774:781	arg1	Reaction					783:790	Maillard Reaction	774:790	Maillard Reaction	774:790	Consequently, browning was more intense due to Maillard Reaction.
34889171	0	17	theme	Dulce	13:17	arg1	leche					22:26	Lactose-free Dulce de leche	0:26	Lactose-free Dulce de leche: compositional characterization, browning and texture profile.	0:89	Lactose-free Dulce de leche: compositional characterization, browning and texture profile.
34889171	8	18	theme	g	1016:1016	arg1	gumminess					1018:1026	406.20 ± 311.69 g gumminess	1000:1026	406.20 ± 311.69 g gumminess	1000:1026	Texture parameters were higher (1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess) when compared to the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess).
34889171	8	19	theme	homogenized	904:914	arg1	product					928:934	the homogenized traditional product	900:934	the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess)	900:1027	Texture parameters were higher (1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess) when compared to the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess).
34889171	6	20	theme	higher	642:647	arg1	concentration					649:661	a higher concentration	640:661	a higher concentration of free 5-Hydroxymethylfurfural (HMF) (133.77 ± 3.42 μmol l-1)	640:724	The main results show that the homogenized lactose-free DL obtained a higher concentration of free 5-Hydroxymethylfurfural (HMF) (133.77 ± 3.42 μmol l-1).
34889171	2	21	theme	enzymatic	316:324	arg1	hydrolysis					326:335	enzymatic hydrolysis	316:335	enzymatic hydrolysis of lactose	316:346	Fluid milk used was divided into two portions and, in one of them, enzymatic hydrolysis of lactose was carried through.
34889171	0	22	theme	Lactose-free	0:11	arg1	leche					22:26	Lactose-free Dulce de leche	0:26	Lactose-free Dulce de leche: compositional characterization, browning and texture profile.	0:89	Lactose-free Dulce de leche: compositional characterization, browning and texture profile.
34889171	1	23	theme	products	239:246	arg1	attributes					221:230	the attributes	217:230	the attributes of the products	217:246	Our objective was to elaborate lactose-free Dulce de leche (DL) and evaluate the influence of the hydrolysis of this sugar on the attributes of the products.
34889171	8	24	dep	higher	817:822	arg1	hardness					844:851	1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess	825:880	hardness	844:851	Texture parameters were higher (1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess) when compared to the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess).
34889171	8	24	dep	higher	817:822	arg1	gumminess					872:880	1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess	825:880	gumminess	872:880	Texture parameters were higher (1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess) when compared to the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess).
34889171	8	25	theme	±	833:833	arg1	hardness					844:851	1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess	825:880	hardness	844:851	Texture parameters were higher (1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess) when compared to the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess).
34889171	8	26	theme	±	976:976	arg1	hardness					986:993	437.17 ± 279.3 g hardness	969:993	437.17 ± 279.3 g hardness	969:993	Texture parameters were higher (1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess) when compared to the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess).
34889171	6	27	theme	μmol	716:719	arg1	5-Hydroxymethylfurfural					671:693	free 5-Hydroxymethylfurfural	666:693	free 5-Hydroxymethylfurfural (HMF) (133.77 ± 3.42 μmol l-1)	666:724	The main results show that the homogenized lactose-free DL obtained a higher concentration of free 5-Hydroxymethylfurfural (HMF) (133.77 ± 3.42 μmol l-1).
34889171	6	27	theme	μmol	716:719	arg1	l-1					721:723	133.77 ± 3.42 μmol l-1	702:723	133.77 ± 3.42 μmol l-1	702:723	The main results show that the homogenized lactose-free DL obtained a higher concentration of free 5-Hydroxymethylfurfural (HMF) (133.77 ± 3.42 μmol l-1).
34889171	3	28	theme	milk	397:400	arg1	homogenization					379:392	the homogenization	375:392	the homogenization of milk	375:400	Next, the homogenization of milk was performed at 20 MPa.
34889171	0	29	theme	de	19:20	arg1	leche					22:26	Lactose-free Dulce de leche	0:26	Lactose-free Dulce de leche: compositional characterization, browning and texture profile.	0:89	Lactose-free Dulce de leche: compositional characterization, browning and texture profile.
34889171	8	30	theme	±	863:863	arg1	gumminess					872:880	1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess	825:880	gumminess	872:880	Texture parameters were higher (1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess) when compared to the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess).
34889171	8	31	theme	g	842:842	arg1	hardness					844:851	1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess	825:880	hardness	844:851	Texture parameters were higher (1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess) when compared to the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess).
34889171	1	32	theme	de	141:142	arg1	leche					144:148	lactose-free Dulce de leche	122:148	lactose-free Dulce de leche (DL)	122:153	Our objective was to elaborate lactose-free Dulce de leche (DL) and evaluate the influence of the hydrolysis of this sugar on the attributes of the products.
34889171	1	32	theme	de	141:142	arg1	DL					151:152	DL	151:152	DL	151:152	Our objective was to elaborate lactose-free Dulce de leche (DL) and evaluate the influence of the hydrolysis of this sugar on the attributes of the products.
34889171	0	33	theme	compositional	29:41	arg1	characterization					43:58	compositional characterization	29:58	compositional characterization	29:58	Lactose-free Dulce de leche: compositional characterization, browning and texture profile.
34889171	9	34	theme	DL	1207:1208	arg1	browning					1168:1175	browning	1168:1175	browning	1168:1175	Lactose-free products are in high demand by consumers; however, the results of this work highlight the challenges to properly control the browning and the texture parameters of DL.
34889171	9	34	theme	DL	1207:1208	arg1	parameters					1193:1202	the texture parameters	1181:1202	the texture parameters	1181:1202	Lactose-free products are in high demand by consumers; however, the results of this work highlight the challenges to properly control the browning and the texture parameters of DL.
34889171	8	35	theme	g	984:984	arg1	hardness					986:993	437.17 ± 279.3 g hardness	969:993	437.17 ± 279.3 g hardness	969:993	Texture parameters were higher (1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess) when compared to the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess).
34889171	6	36	theme	lactose-free	615:626	arg1	DL					628:629	the homogenized lactose-free DL	599:629	the homogenized lactose-free DL	599:629	The main results show that the homogenized lactose-free DL obtained a higher concentration of free 5-Hydroxymethylfurfural (HMF) (133.77 ± 3.42 μmol l-1).
34889171	5	37	theme	final	471:475	arg1	products					477:484	The final products	467:484	The final products	467:484	The final products were evaluated in relation to their composition and physico-chemical characteristics.
34889171	1	38	theme	hydrolysis	189:198	arg1	influence					172:180	the influence	168:180	the influence of the hydrolysis of this sugar on the attributes of the products	168:246	Our objective was to elaborate lactose-free Dulce de leche (DL) and evaluate the influence of the hydrolysis of this sugar on the attributes of the products.
34889171	6	39	theme	±	709:709	arg1	5-Hydroxymethylfurfural					671:693	free 5-Hydroxymethylfurfural	666:693	free 5-Hydroxymethylfurfural (HMF) (133.77 ± 3.42 μmol l-1)	666:724	The main results show that the homogenized lactose-free DL obtained a higher concentration of free 5-Hydroxymethylfurfural (HMF) (133.77 ± 3.42 μmol l-1).
34889171	6	39	theme	±	709:709	arg1	l-1					721:723	133.77 ± 3.42 μmol l-1	702:723	133.77 ± 3.42 μmol l-1	702:723	The main results show that the homogenized lactose-free DL obtained a higher concentration of free 5-Hydroxymethylfurfural (HMF) (133.77 ± 3.42 μmol l-1).
34889171	1	40	from	influence	172:180	arg1	attributes					221:230	the attributes	217:230	the attributes of the products	217:246	Our objective was to elaborate lactose-free Dulce de leche (DL) and evaluate the influence of the hydrolysis of this sugar on the attributes of the products.
34889171	6	41	theme	homogenized	603:613	arg1	DL					628:629	the homogenized lactose-free DL	599:629	the homogenized lactose-free DL	599:629	The main results show that the homogenized lactose-free DL obtained a higher concentration of free 5-Hydroxymethylfurfural (HMF) (133.77 ± 3.42 μmol l-1).
34889171	9	42	dep	browning	1168:1175	arg1	the					1164:1166	the	1164:1166	the	1164:1166	Lactose-free products are in high demand by consumers; however, the results of this work highlight the challenges to properly control the browning and the texture parameters of DL.
34889171	9	43	theme	work	1114:1117	arg1	results					1098:1104	the results	1094:1104	the results of this work	1094:1117	Lactose-free products are in high demand by consumers; however, the results of this work highlight the challenges to properly control the browning and the texture parameters of DL.
34889171	0	44	dep	leche	22:26	arg1	browning					61:68	browning	61:68	browning	61:68	Lactose-free Dulce de leche: compositional characterization, browning and texture profile.
34889171	0	44	dep	leche	22:26	arg1	profile					82:88	texture profile	74:88	texture profile	74:88	Lactose-free Dulce de leche: compositional characterization, browning and texture profile.
34889171	0	44	dep	leche	22:26	arg1	characterization					43:58	compositional characterization	29:58	compositional characterization	29:58	Lactose-free Dulce de leche: compositional characterization, browning and texture profile.
34889171	1	45	theme	lactose-free	122:133	arg1	leche					144:148	lactose-free Dulce de leche	122:148	lactose-free Dulce de leche (DL)	122:153	Our objective was to elaborate lactose-free Dulce de leche (DL) and evaluate the influence of the hydrolysis of this sugar on the attributes of the products.
34889171	1	45	theme	lactose-free	122:133	arg1	DL					151:152	DL	151:152	DL	151:152	Our objective was to elaborate lactose-free Dulce de leche (DL) and evaluate the influence of the hydrolysis of this sugar on the attributes of the products.
34889171	8	46	theme	g	870:870	arg1	gumminess					872:880	1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess	825:880	gumminess	872:880	Texture parameters were higher (1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess) when compared to the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess).
34889171	1	47	theme	Dulce	135:139	arg1	leche					144:148	lactose-free Dulce de leche	122:148	lactose-free Dulce de leche (DL)	122:153	Our objective was to elaborate lactose-free Dulce de leche (DL) and evaluate the influence of the hydrolysis of this sugar on the attributes of the products.
34889171	1	47	theme	Dulce	135:139	arg1	DL					151:152	DL	151:152	DL	151:152	Our objective was to elaborate lactose-free Dulce de leche (DL) and evaluate the influence of the hydrolysis of this sugar on the attributes of the products.
34889171	2	48	theme	lactose	340:346	arg1	hydrolysis					326:335	enzymatic hydrolysis	316:335	enzymatic hydrolysis of lactose	316:346	Fluid milk used was divided into two portions and, in one of them, enzymatic hydrolysis of lactose was carried through.
34889171	2	49	theme	them	310:313	arg1	one					303:305	one	303:305	one	303:305	Fluid milk used was divided into two portions and, in one of them, enzymatic hydrolysis of lactose was carried through.
34889171	2	49	theme	them	310:313	arg1	them					310:313	them	310:313	them	310:313	Fluid milk used was divided into two portions and, in one of them, enzymatic hydrolysis of lactose was carried through.
34889171	1	50	theme	sugar	208:212	arg1	hydrolysis					189:198	the hydrolysis	185:198	the hydrolysis of this sugar	185:212	Our objective was to elaborate lactose-free Dulce de leche (DL) and evaluate the influence of the hydrolysis of this sugar on the attributes of the products.
34889171	8	51	theme	±	1007:1007	arg1	gumminess					1018:1026	406.20 ± 311.69 g gumminess	1000:1026	406.20 ± 311.69 g gumminess	1000:1026	Texture parameters were higher (1611.00 ± 598.78 g hardness and 19.52 ± 2.46 g gumminess) when compared to the homogenized traditional product (28.45 ± 1.16 μmol l-1 free HMF, 437.17 ± 279.3 g hardness, and 406.20 ± 311.69 g gumminess).
34889171	9	52	theme	Lactose-free	1030:1041	arg1	products					1043:1050	Lactose-free products	1030:1050	Lactose-free products	1030:1050	Lactose-free products are in high demand by consumers; however, the results of this work highlight the challenges to properly control the browning and the texture parameters of DL.
32603191	6	0	theme	1→4	949:951	arg1	residues					970:977	(1→4)-linked-α-D-Glcp residues	948:977	(1→4)-linked-α-D-Glcp residues	948:977	The backbone of MCGP-L was composed of (1→4)-linked-α-D-Glcp residues and with branch chain substituted at O-6 position of (1→4,6)-linked-α-D-Glcp.
32603191	2	1	theme	Sephadex	363:370	arg1	column					377:382	the Sephadex G-25 column	359:382	the Sephadex G-25 column	359:382	The polysaccharide MCGP-L was purified by molecular exclusion chromatography using the Sephadex G-25 column.
32603191	5	2	theme	High	825:828	arg1	Chromatography					849:862	High Performance Liquid Chromatography	825:862	High Performance Liquid Chromatography (HPLC)	825:869	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	5	2	theme	High	825:828	arg1	HPLC					865:868	HPLC	865:868	HPLC	865:868	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	5	3	theme	structural	627:636	arg1	characteristics					638:652	structural characteristics	627:652	structural characteristics	627:652	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	4	4	theme	composition	472:482	arg1	analysis					484:491	Monosaccharide composition analysis	457:491	Monosaccharide composition analysis	457:491	Monosaccharide composition analysis showed MCGP-L was composed of three kinds of monosaccharide: D-glucose, D-galactose and D-mannose.
32603191	5	5	theme	Performance	774:784	arg1	Chromatography					801:814	High Performance Gel-Permeation Chromatography	769:814	High Performance Gel-Permeation Chromatography (HPGPC)	769:822	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	5	5	theme	Performance	774:784	arg1	HPGPC					817:821	HPGPC	817:821	HPGPC	817:821	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	0	6	from	Isolation	0:8	arg1	roots					87:91	the roots	83:91	the roots of Panax ginseng C. A. Meyer	83:120	Isolation and structural elucidation of a low-molecular-weight polysaccharide from the roots of Panax ginseng C. A. Meyer.
32603191	5	7	theme	chemical	704:711	arg1	combination					689:699	the combination	685:699	the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR)	685:906	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	7	8	dep	-α-D-Manp-	1107:1116	arg1	β-D-Glcp-					1125:1133	β-D-Glcp-	1125:1133	β-D-Glcp-	1125:1133	The branch chain consists of →6)-α-D-Galp-(1→, →2)-α-D-Manp-(1→ and β-D-Glcp-(1→.
32603191	7	8	dep	-α-D-Manp-	1107:1116	arg1	1→					1118:1119	1→	1118:1119	1→	1118:1119	The branch chain consists of →6)-α-D-Galp-(1→, →2)-α-D-Manp-(1→ and β-D-Glcp-(1→.
32603191	2	9	theme	polysaccharide	280:293	arg1	MCGP-L					295:300	The polysaccharide MCGP-L	276:300	The polysaccharide MCGP-L	276:300	The polysaccharide MCGP-L was purified by molecular exclusion chromatography using the Sephadex G-25 column.
32603191	0	10	theme	Panax	96:100	arg1	roots					87:91	the roots	83:91	the roots of Panax ginseng C. A. Meyer	83:120	Isolation and structural elucidation of a low-molecular-weight polysaccharide from the roots of Panax ginseng C. A. Meyer.
32603191	2	11	theme	molecular	318:326	arg1	chromatography					338:351	molecular exclusion chromatography	318:351	molecular exclusion chromatography using the Sephadex G-25 column	318:382	The polysaccharide MCGP-L was purified by molecular exclusion chromatography using the Sephadex G-25 column.
32603191	5	12	theme	MCGP-L	657:662	arg1	properties					612:621	physicochemical properties	596:621	physicochemical properties	596:621	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	5	12	theme	MCGP-L	657:662	arg1	characteristics					638:652	structural characteristics	627:652	structural characteristics	627:652	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	6	13	theme	O-6	1016:1018	arg1	position					1020:1027	O-6 position	1016:1027	O-6 position of (1→4,6)-linked-α-D-Glcp	1016:1054	The backbone of MCGP-L was composed of (1→4)-linked-α-D-Glcp residues and with branch chain substituted at O-6 position of (1→4,6)-linked-α-D-Glcp.
32603191	6	14	theme	MCGP-L	925:930	arg1	backbone					913:920	The backbone	909:920	The backbone of MCGP-L	909:930	The backbone of MCGP-L was composed of (1→4)-linked-α-D-Glcp residues and with branch chain substituted at O-6 position of (1→4,6)-linked-α-D-Glcp.
32603191	5	15	theme	Performance	830:840	arg1	Chromatography					849:862	High Performance Liquid Chromatography	825:862	High Performance Liquid Chromatography (HPLC)	825:869	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	5	15	theme	Performance	830:840	arg1	HPLC					865:868	HPLC	865:868	HPLC	865:868	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	6	16	theme	-linked-α-D-Glcp	1039:1054	arg1	position					1020:1027	O-6 position	1016:1027	O-6 position of (1→4,6)-linked-α-D-Glcp	1016:1054	The backbone of MCGP-L was composed of (1→4)-linked-α-D-Glcp residues and with branch chain substituted at O-6 position of (1→4,6)-linked-α-D-Glcp.
32603191	3	17	theme	average	389:395	arg1	3 × 103 kDa					444:454	3 × 103 kDa	444:454	3 × 103 kDa	444:454	The average molecular weight of MCGP-L was estimated to be 3 × 103 kDa.
32603191	3	17	theme	average	389:395	arg1	weight					407:412	The average molecular weight	385:412	The average molecular weight of MCGP-L	385:422	The average molecular weight of MCGP-L was estimated to be 3 × 103 kDa.
32603191	6	18	theme	branch	988:993	arg1	chain					995:999	branch chain	988:999	branch chain substituted at O-6 position of (1→4,6)-linked-α-D-Glcp	988:1054	The backbone of MCGP-L was composed of (1→4)-linked-α-D-Glcp residues and with branch chain substituted at O-6 position of (1→4,6)-linked-α-D-Glcp.
32603191	5	19	theme	physicochemical	596:610	arg1	properties					612:621	physicochemical properties	596:621	physicochemical properties	596:621	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	5	20	theme	analysis	730:737	arg1	combination					689:699	the combination	685:699	the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR)	685:906	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	3	21	theme	molecular	397:405	arg1	3 × 103 kDa					444:454	3 × 103 kDa	444:454	3 × 103 kDa	444:454	The average molecular weight of MCGP-L was estimated to be 3 × 103 kDa.
32603191	3	21	theme	molecular	397:405	arg1	weight					407:412	The average molecular weight	385:412	The average molecular weight of MCGP-L	385:422	The average molecular weight of MCGP-L was estimated to be 3 × 103 kDa.
32603191	4	22	dep	kinds	529:533	arg1	D-glucose					554:562	D-glucose	554:562	D-glucose	554:562	Monosaccharide composition analysis showed MCGP-L was composed of three kinds of monosaccharide: D-glucose, D-galactose and D-mannose.
32603191	4	22	dep	kinds	529:533	arg1	kinds					529:533	three kinds	523:533	three kinds of monosaccharide: D-glucose, D-galactose and D-mannose	523:589	Monosaccharide composition analysis showed MCGP-L was composed of three kinds of monosaccharide: D-glucose, D-galactose and D-mannose.
32603191	4	22	dep	kinds	529:533	arg1	D-mannose					581:589	D-mannose	581:589	D-mannose	581:589	Monosaccharide composition analysis showed MCGP-L was composed of three kinds of monosaccharide: D-glucose, D-galactose and D-mannose.
32603191	4	22	dep	kinds	529:533	arg1	D-galactose					565:575	D-galactose	565:575	D-galactose	565:575	Monosaccharide composition analysis showed MCGP-L was composed of three kinds of monosaccharide: D-glucose, D-galactose and D-mannose.
32603191	5	23	theme	Magnetic	883:890	arg1	NMR					903:905	NMR	903:905	NMR	903:905	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	5	23	theme	Magnetic	883:890	arg1	Resonance					892:900	Nuclear Magnetic Resonance	875:900	Nuclear Magnetic Resonance (NMR)	875:906	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	0	24	theme	structural	14:23	arg1	elucidation					25:35	structural elucidation	14:35	structural elucidation	14:35	Isolation and structural elucidation of a low-molecular-weight polysaccharide from the roots of Panax ginseng C. A. Meyer.
32603191	7	25	theme	-α-D-Galp-	1089:1098	arg1	-α-D-Manp-					1107:1116	→6)-α-D-Galp-(1→, →2)-α-D-Manp-(1→ and β-D-Glcp-(1→	1086:1136	-α-D-Manp-	1107:1116	The branch chain consists of →6)-α-D-Galp-(1→, →2)-α-D-Manp-(1→ and β-D-Glcp-(1→.
32603191	4	26	theme	Monosaccharide	457:470	arg1	analysis					484:491	Monosaccharide composition analysis	457:491	Monosaccharide composition analysis	457:491	Monosaccharide composition analysis showed MCGP-L was composed of three kinds of monosaccharide: D-glucose, D-galactose and D-mannose.
32603191	3	27	theme	MCGP-L	417:422	arg1	3 × 103 kDa					444:454	3 × 103 kDa	444:454	3 × 103 kDa	444:454	The average molecular weight of MCGP-L was estimated to be 3 × 103 kDa.
32603191	3	27	theme	MCGP-L	417:422	arg1	weight					407:412	The average molecular weight	385:412	The average molecular weight of MCGP-L	385:422	The average molecular weight of MCGP-L was estimated to be 3 × 103 kDa.
32603191	4	28	dep	showed	493:498	arg1	composed					511:518	composed	511:518	showed MCGP-L was composed of three kinds of monosaccharide: D-glucose, D-galactose and D-mannose	493:589	Monosaccharide composition analysis showed MCGP-L was composed of three kinds of monosaccharide: D-glucose, D-galactose and D-mannose.
32603191	0	29	from	roots	87:91	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and structural elucidation of a low-molecular-weight polysaccharide from the roots of Panax ginseng C. A. Meyer.
32603191	0	29	from	roots	87:91	arg1	elucidation					25:35	structural elucidation	14:35	structural elucidation	14:35	Isolation and structural elucidation of a low-molecular-weight polysaccharide from the roots of Panax ginseng C. A. Meyer.
32603191	0	29	from	roots	87:91	arg1	polysaccharide					63:76	a low-molecular-weight polysaccharide	40:76	a low-molecular-weight polysaccharide from the roots of Panax ginseng C. A. Meyer	40:120	Isolation and structural elucidation of a low-molecular-weight polysaccharide from the roots of Panax ginseng C. A. Meyer.
32603191	5	30	theme	Liquid	842:847	arg1	Chromatography					849:862	High Performance Liquid Chromatography	825:862	High Performance Liquid Chromatography (HPLC)	825:869	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	5	30	theme	Liquid	842:847	arg1	HPLC					865:868	HPLC	865:868	HPLC	865:868	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	5	31	theme	High	769:772	arg1	Chromatography					801:814	High Performance Gel-Permeation Chromatography	769:814	High Performance Gel-Permeation Chromatography (HPGPC)	769:822	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	5	31	theme	High	769:772	arg1	HPGPC					817:821	HPGPC	817:821	HPGPC	817:821	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	5	32	theme	Gel-Permeation	786:799	arg1	Chromatography					801:814	High Performance Gel-Permeation Chromatography	769:814	High Performance Gel-Permeation Chromatography (HPGPC)	769:822	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	5	32	theme	Gel-Permeation	786:799	arg1	HPGPC					817:821	HPGPC	817:821	HPGPC	817:821	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	6	33	theme	1→4,6	1033:1037	arg1	-linked-α-D-Glcp					1039:1054	(1→4,6)-linked-α-D-Glcp	1032:1054	(1→4,6)-linked-α-D-Glcp	1032:1054	The backbone of MCGP-L was composed of (1→4)-linked-α-D-Glcp residues and with branch chain substituted at O-6 position of (1→4,6)-linked-α-D-Glcp.
32603191	1	34	theme	Mountain	219:226	arg1	ginseng					239:245	Mountain cultivated ginseng	219:245	Mountain cultivated ginseng (Panax ginseng C. A. Meyer)	219:273	A low-molecular-weight polysaccharide named MCGP-L was extracted and purified from the roots of Mountain cultivated ginseng (Panax ginseng C. A. Meyer).
32603191	0	35	theme	polysaccharide	63:76	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and structural elucidation of a low-molecular-weight polysaccharide from the roots of Panax ginseng C. A. Meyer.
32603191	0	35	theme	polysaccharide	63:76	arg1	elucidation					25:35	structural elucidation	14:35	structural elucidation	14:35	Isolation and structural elucidation of a low-molecular-weight polysaccharide from the roots of Panax ginseng C. A. Meyer.
32603191	4	36	theme	monosaccharide	538:551	arg1	D-glucose					554:562	D-glucose	554:562	D-glucose	554:562	Monosaccharide composition analysis showed MCGP-L was composed of three kinds of monosaccharide: D-glucose, D-galactose and D-mannose.
32603191	4	36	theme	monosaccharide	538:551	arg1	D-mannose					581:589	D-mannose	581:589	D-mannose	581:589	Monosaccharide composition analysis showed MCGP-L was composed of three kinds of monosaccharide: D-glucose, D-galactose and D-mannose.
32603191	4	36	theme	monosaccharide	538:551	arg1	kinds					529:533	three kinds	523:533	three kinds of monosaccharide: D-glucose, D-galactose and D-mannose	523:589	Monosaccharide composition analysis showed MCGP-L was composed of three kinds of monosaccharide: D-glucose, D-galactose and D-mannose.
32603191	4	36	theme	monosaccharide	538:551	arg1	D-galactose					565:575	D-galactose	565:575	D-galactose	565:575	Monosaccharide composition analysis showed MCGP-L was composed of three kinds of monosaccharide: D-glucose, D-galactose and D-mannose.
32603191	5	37	theme	instrumental	717:728	arg1	analysis					730:737	instrumental analysis	717:737	instrumental analysis	717:737	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	1	38	dep	ginseng	239:245	arg1	ginseng					254:260	Panax ginseng	248:260	Panax ginseng C. A. Meyer	248:272	A low-molecular-weight polysaccharide named MCGP-L was extracted and purified from the roots of Mountain cultivated ginseng (Panax ginseng C. A. Meyer).
32603191	7	39	dep	1→	1118:1119	arg1	1→					1135:1136	1→	1135:1136	1→	1135:1136	The branch chain consists of →6)-α-D-Galp-(1→, →2)-α-D-Manp-(1→ and β-D-Glcp-(1→.
32603191	0	40	theme	low-molecular-weight	42:61	arg1	polysaccharide					63:76	a low-molecular-weight polysaccharide	40:76	a low-molecular-weight polysaccharide from the roots of Panax ginseng C. A. Meyer	40:120	Isolation and structural elucidation of a low-molecular-weight polysaccharide from the roots of Panax ginseng C. A. Meyer.
32603191	5	41	theme	methylation	747:757	arg1	analysis					759:766	methylation analysis	747:766	methylation analysis	747:766	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	6	42	theme	-linked-α-D-Glcp	953:968	arg1	residues					970:977	(1→4)-linked-α-D-Glcp residues	948:977	(1→4)-linked-α-D-Glcp residues	948:977	The backbone of MCGP-L was composed of (1→4)-linked-α-D-Glcp residues and with branch chain substituted at O-6 position of (1→4,6)-linked-α-D-Glcp.
32603191	2	43	theme	exclusion	328:336	arg1	chromatography					338:351	molecular exclusion chromatography	318:351	molecular exclusion chromatography using the Sephadex G-25 column	318:382	The polysaccharide MCGP-L was purified by molecular exclusion chromatography using the Sephadex G-25 column.
32603191	1	44	theme	cultivated	228:237	arg1	ginseng					239:245	Mountain cultivated ginseng	219:245	Mountain cultivated ginseng (Panax ginseng C. A. Meyer)	219:273	A low-molecular-weight polysaccharide named MCGP-L was extracted and purified from the roots of Mountain cultivated ginseng (Panax ginseng C. A. Meyer).
32603191	7	45	theme	branch	1061:1066	arg1	chain					1068:1072	The branch chain	1057:1072	The branch chain	1057:1072	The branch chain consists of →6)-α-D-Galp-(1→, →2)-α-D-Manp-(1→ and β-D-Glcp-(1→.
32603191	1	46	dep	ginseng	254:260	arg1	Meyer					268:272	Meyer	268:272	Meyer	268:272	A low-molecular-weight polysaccharide named MCGP-L was extracted and purified from the roots of Mountain cultivated ginseng (Panax ginseng C. A. Meyer).
32603191	1	47	theme	low-molecular-weight	125:144	arg1	polysaccharide					146:159	A low-molecular-weight polysaccharide	123:159	A low-molecular-weight polysaccharide named MCGP-L	123:172	A low-molecular-weight polysaccharide named MCGP-L was extracted and purified from the roots of Mountain cultivated ginseng (Panax ginseng C. A. Meyer).
32603191	1	48	theme	ginseng	239:245	arg1	roots					210:214	the roots	206:214	the roots of Mountain cultivated ginseng (Panax ginseng C. A. Meyer)	206:273	A low-molecular-weight polysaccharide named MCGP-L was extracted and purified from the roots of Mountain cultivated ginseng (Panax ginseng C. A. Meyer).
32603191	0	49	from	elucidation	25:35	arg1	roots					87:91	the roots	83:91	the roots of Panax ginseng C. A. Meyer	83:120	Isolation and structural elucidation of a low-molecular-weight polysaccharide from the roots of Panax ginseng C. A. Meyer.
32603191	5	50	theme	Nuclear	875:881	arg1	NMR					903:905	NMR	903:905	NMR	903:905	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	5	50	theme	Nuclear	875:881	arg1	Resonance					892:900	Nuclear Magnetic Resonance	875:900	Nuclear Magnetic Resonance (NMR)	875:906	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	2	51	theme	G-25	372:375	arg1	column					377:382	the Sephadex G-25 column	359:382	the Sephadex G-25 column	359:382	The polysaccharide MCGP-L was purified by molecular exclusion chromatography using the Sephadex G-25 column.
32603191	5	52	dep	properties	612:621	arg1	The					592:594	The	592:594	The	592:594	The physicochemical properties and structural characteristics of MCGP-L were investigated by the combination of chemical and instrumental analysis such as methylation analysis, High Performance Gel-Permeation Chromatography (HPGPC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).
32603191	1	53	theme	Panax	248:252	arg1	ginseng					254:260	Panax ginseng	248:260	Panax ginseng C. A. Meyer	248:272	A low-molecular-weight polysaccharide named MCGP-L was extracted and purified from the roots of Mountain cultivated ginseng (Panax ginseng C. A. Meyer).
34973768	5	0	theme	monosaccharide	756:769	arg1	composition					771:781	monosaccharide composition	756:781	monosaccharide composition	756:781	Applying this combinatorial approach, the monosaccharide composition, sequence and linkage pattern of this novel polymer was determined via HPLC-MS, GC-MS and NMR.
34973768	5	1	theme	polymer	827:833	arg1	pattern					805:811	the monosaccharide composition, sequence and linkage pattern	752:811	the monosaccharide composition, sequence and linkage pattern of this novel polymer	752:833	Applying this combinatorial approach, the monosaccharide composition, sequence and linkage pattern of this novel polymer was determined via HPLC-MS, GC-MS and NMR.
34973768	1	2	theme	industrial	166:175	arg1	interest					177:184	industrial interest	166:184	industrial interest	166:184	Paenibacillus polymyxa is an avid producer of exopolysaccharides of industrial interest.
34973768	0	3	theme	DSM	89:91	arg1	polysaccharide					48:61	polysaccharide	48:61	polysaccharide of Paenibacillus polymyxa DSM 365	48:95	Structural elucidation of the fucose containing polysaccharide of Paenibacillus polymyxa DSM 365.
34973768	1	4	theme	interest	177:184	arg1	exopolysaccharides					144:161	exopolysaccharides	144:161	exopolysaccharides of industrial interest	144:184	Paenibacillus polymyxa is an avid producer of exopolysaccharides of industrial interest.
34973768	6	5	theme	polymerase	1002:1011	arg1	PepG					1013:1016	the Wzy polymerase PepG	994:1016	the Wzy polymerase PepG	994:1016	Furthermore, we demonstrated that the knock-out of the glycosyltransferases PepQ, PepT, PepU and PepV as well as of the Wzy polymerase PepG led to the absence of paenan II, attributing those enzymes to the assembly of the repeating unit.
34973768	3	6	theme	mediated	351:358	arg1	strategy					370:377	a CRISPR-Cas9 mediated knock-out strategy	337:377	a CRISPR-Cas9 mediated knock-out strategy	337:377	By using a CRISPR-Cas9 mediated knock-out strategy, all single glycosyltransferases as well as the Wzy polymerases were individually deleted in the corresponding gene cluster for the first time.
34973768	6	7	theme	PepG	1013:1016	arg1	knock-out					916:924	the knock-out	912:924	the knock-out of the glycosyltransferases PepQ, PepT, PepU and PepV as well as of the Wzy polymerase PepG	912:1016	Furthermore, we demonstrated that the knock-out of the glycosyltransferases PepQ, PepT, PepU and PepV as well as of the Wzy polymerase PepG led to the absence of paenan II, attributing those enzymes to the assembly of the repeating unit.
34973768	5	8	theme	sequence	784:791	arg1	pattern					805:811	the monosaccharide composition, sequence and linkage pattern	752:811	the monosaccharide composition, sequence and linkage pattern of this novel polymer	752:833	Applying this combinatorial approach, the monosaccharide composition, sequence and linkage pattern of this novel polymer was determined via HPLC-MS, GC-MS and NMR.
34973768	3	9	theme	knock-out	360:368	arg1	strategy					370:377	a CRISPR-Cas9 mediated knock-out strategy	337:377	a CRISPR-Cas9 mediated knock-out strategy	337:377	By using a CRISPR-Cas9 mediated knock-out strategy, all single glycosyltransferases as well as the Wzy polymerases were individually deleted in the corresponding gene cluster for the first time.
34973768	2	10	theme	polysaccharide	276:289	arg1	elucidation					257:267	structural elucidation	246:267	structural elucidation of the polysaccharide	246:289	However, due to the complexity of the polymer composition, structural elucidation of the polysaccharide remained unfeasible for a long time.
34973768	4	11	theme	containing	642:651	arg1	polysaccharide					653:666	a pure minor fucose containing polysaccharide	622:666	a pure minor fucose containing polysaccharide	622:666	Thereby, it was observed that the main polymer fraction was completely suppressed (or deleted) and a pure minor fucose containing polysaccharide could be isolated, which was named paenan II.
34973768	4	12	theme	polymer	562:568	arg1	fraction					570:577	the main polymer fraction	553:577	the main polymer fraction	553:577	Thereby, it was observed that the main polymer fraction was completely suppressed (or deleted) and a pure minor fucose containing polysaccharide could be isolated, which was named paenan II.
34973768	6	13	theme	repeating	1100:1108	arg1	unit					1110:1113	the repeating unit	1096:1113	the repeating unit	1096:1113	Furthermore, we demonstrated that the knock-out of the glycosyltransferases PepQ, PepT, PepU and PepV as well as of the Wzy polymerase PepG led to the absence of paenan II, attributing those enzymes to the assembly of the repeating unit.
34973768	0	14	theme	Structural	0:9	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation of the fucose	0:35	Structural elucidation of the fucose containing polysaccharide of Paenibacillus polymyxa DSM 365.
34973768	4	15	theme	main	557:560	arg1	fraction					570:577	the main polymer fraction	553:577	the main polymer fraction	553:577	Thereby, it was observed that the main polymer fraction was completely suppressed (or deleted) and a pure minor fucose containing polysaccharide could be isolated, which was named paenan II.
34973768	1	16	theme	Paenibacillus	98:110	arg1	producer					132:139	an avid producer	124:139	an avid producer of exopolysaccharides of industrial interest	124:184	Paenibacillus polymyxa is an avid producer of exopolysaccharides of industrial interest.
34973768	1	16	theme	Paenibacillus	98:110	arg1	polymyxa					112:119	Paenibacillus polymyxa	98:119	Paenibacillus polymyxa	98:119	Paenibacillus polymyxa is an avid producer of exopolysaccharides of industrial interest.
34973768	2	17	theme	long	317:320	arg1	time					322:325	a long time	315:325	a long time	315:325	However, due to the complexity of the polymer composition, structural elucidation of the polysaccharide remained unfeasible for a long time.
34973768	3	18	theme	corresponding	476:488	arg1	cluster					495:501	the corresponding gene cluster	472:501	the corresponding gene cluster for the first time	472:520	By using a CRISPR-Cas9 mediated knock-out strategy, all single glycosyltransferases as well as the Wzy polymerases were individually deleted in the corresponding gene cluster for the first time.
34973768	3	19	theme	single	384:389	arg1	glycosyltransferases					391:410	all single glycosyltransferases	380:410	all single glycosyltransferases as well as the Wzy polymerases	380:441	By using a CRISPR-Cas9 mediated knock-out strategy, all single glycosyltransferases as well as the Wzy polymerases were individually deleted in the corresponding gene cluster for the first time.
34973768	6	20	theme	paenan	1040:1045	arg1	absence					1029:1035	the absence	1025:1035	the absence of paenan II	1025:1048	Furthermore, we demonstrated that the knock-out of the glycosyltransferases PepQ, PepT, PepU and PepV as well as of the Wzy polymerase PepG led to the absence of paenan II, attributing those enzymes to the assembly of the repeating unit.
34973768	3	21	theme	gene	490:493	arg1	cluster					495:501	the corresponding gene cluster	472:501	the corresponding gene cluster for the first time	472:520	By using a CRISPR-Cas9 mediated knock-out strategy, all single glycosyltransferases as well as the Wzy polymerases were individually deleted in the corresponding gene cluster for the first time.
34973768	3	22	theme	first	511:515	arg1	time					517:520	the first time	507:520	the first time	507:520	By using a CRISPR-Cas9 mediated knock-out strategy, all single glycosyltransferases as well as the Wzy polymerases were individually deleted in the corresponding gene cluster for the first time.
34973768	4	23	dep	suppressed	594:603	arg1	deleted					609:615	deleted	609:615	deleted	609:615	Thereby, it was observed that the main polymer fraction was completely suppressed (or deleted) and a pure minor fucose containing polysaccharide could be isolated, which was named paenan II.
34973768	4	24	theme	fucose	635:640	arg1	polysaccharide					653:666	a pure minor fucose containing polysaccharide	622:666	a pure minor fucose containing polysaccharide	622:666	Thereby, it was observed that the main polymer fraction was completely suppressed (or deleted) and a pure minor fucose containing polysaccharide could be isolated, which was named paenan II.
34973768	0	25	theme	fucose	30:35	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation of the fucose	0:35	Structural elucidation of the fucose containing polysaccharide of Paenibacillus polymyxa DSM 365.
34973768	6	26	theme	glycosyltransferases	933:952	arg1	knock-out					916:924	the knock-out	912:924	the knock-out of the glycosyltransferases PepQ, PepT, PepU and PepV as well as of the Wzy polymerase PepG	912:1016	Furthermore, we demonstrated that the knock-out of the glycosyltransferases PepQ, PepT, PepU and PepV as well as of the Wzy polymerase PepG led to the absence of paenan II, attributing those enzymes to the assembly of the repeating unit.
34973768	3	27	theme	Wzy	427:429	arg1	polymerases					431:441	the Wzy polymerases	423:441	all single glycosyltransferases as well as the Wzy polymerases	380:441	By using a CRISPR-Cas9 mediated knock-out strategy, all single glycosyltransferases as well as the Wzy polymerases were individually deleted in the corresponding gene cluster for the first time.
34973768	6	28	dep	glycosyltransferases	933:952	arg1	PepV					975:978	PepV	975:978	PepV	975:978	Furthermore, we demonstrated that the knock-out of the glycosyltransferases PepQ, PepT, PepU and PepV as well as of the Wzy polymerase PepG led to the absence of paenan II, attributing those enzymes to the assembly of the repeating unit.
34973768	6	28	dep	glycosyltransferases	933:952	arg1	PepU					966:969	PepU	966:969	PepU	966:969	Furthermore, we demonstrated that the knock-out of the glycosyltransferases PepQ, PepT, PepU and PepV as well as of the Wzy polymerase PepG led to the absence of paenan II, attributing those enzymes to the assembly of the repeating unit.
34973768	6	28	dep	glycosyltransferases	933:952	arg1	glycosyltransferases					933:952	the glycosyltransferases	929:952	the glycosyltransferases PepQ, PepT, PepU and PepV as well as of the Wzy polymerase PepG	929:1016	Furthermore, we demonstrated that the knock-out of the glycosyltransferases PepQ, PepT, PepU and PepV as well as of the Wzy polymerase PepG led to the absence of paenan II, attributing those enzymes to the assembly of the repeating unit.
34973768	6	28	dep	glycosyltransferases	933:952	arg1	PepT					960:963	PepT	960:963	PepT	960:963	Furthermore, we demonstrated that the knock-out of the glycosyltransferases PepQ, PepT, PepU and PepV as well as of the Wzy polymerase PepG led to the absence of paenan II, attributing those enzymes to the assembly of the repeating unit.
34973768	6	28	dep	glycosyltransferases	933:952	arg1	PepQ					954:957	PepQ	954:957	PepQ	954:957	Furthermore, we demonstrated that the knock-out of the glycosyltransferases PepQ, PepT, PepU and PepV as well as of the Wzy polymerase PepG led to the absence of paenan II, attributing those enzymes to the assembly of the repeating unit.
34973768	2	29	theme	composition	233:243	arg1	complexity					207:216	the complexity	203:216	the complexity of the polymer composition	203:243	However, due to the complexity of the polymer composition, structural elucidation of the polysaccharide remained unfeasible for a long time.
34973768	4	30	theme	minor	629:633	arg1	polysaccharide					653:666	a pure minor fucose containing polysaccharide	622:666	a pure minor fucose containing polysaccharide	622:666	Thereby, it was observed that the main polymer fraction was completely suppressed (or deleted) and a pure minor fucose containing polysaccharide could be isolated, which was named paenan II.
34973768	2	31	theme	polymer	225:231	arg1	composition					233:243	the polymer composition	221:243	the polymer composition	221:243	However, due to the complexity of the polymer composition, structural elucidation of the polysaccharide remained unfeasible for a long time.
34973768	5	32	theme	linkage	797:803	arg1	pattern					805:811	the monosaccharide composition, sequence and linkage pattern	752:811	the monosaccharide composition, sequence and linkage pattern of this novel polymer	752:833	Applying this combinatorial approach, the monosaccharide composition, sequence and linkage pattern of this novel polymer was determined via HPLC-MS, GC-MS and NMR.
34973768	1	33	theme	avid	127:130	arg1	producer					132:139	an avid producer	124:139	an avid producer of exopolysaccharides of industrial interest	124:184	Paenibacillus polymyxa is an avid producer of exopolysaccharides of industrial interest.
34973768	1	33	theme	avid	127:130	arg1	polymyxa					112:119	Paenibacillus polymyxa	98:119	Paenibacillus polymyxa	98:119	Paenibacillus polymyxa is an avid producer of exopolysaccharides of industrial interest.
34973768	5	34	theme	combinatorial	728:740	arg1	approach					742:749	this combinatorial approach	723:749	this combinatorial approach	723:749	Applying this combinatorial approach, the monosaccharide composition, sequence and linkage pattern of this novel polymer was determined via HPLC-MS, GC-MS and NMR.
34973768	6	35	theme	unit	1110:1113	arg1	assembly					1084:1091	the assembly	1080:1091	the assembly of the repeating unit	1080:1113	Furthermore, we demonstrated that the knock-out of the glycosyltransferases PepQ, PepT, PepU and PepV as well as of the Wzy polymerase PepG led to the absence of paenan II, attributing those enzymes to the assembly of the repeating unit.
34973768	5	36	theme	novel	821:825	arg1	polymer					827:833	this novel polymer	816:833	this novel polymer	816:833	Applying this combinatorial approach, the monosaccharide composition, sequence and linkage pattern of this novel polymer was determined via HPLC-MS, GC-MS and NMR.
34973768	5	37	theme	composition	771:781	arg1	pattern					805:811	the monosaccharide composition, sequence and linkage pattern	752:811	the monosaccharide composition, sequence and linkage pattern of this novel polymer	752:833	Applying this combinatorial approach, the monosaccharide composition, sequence and linkage pattern of this novel polymer was determined via HPLC-MS, GC-MS and NMR.
34973768	0	38	theme	polymyxa	80:87	arg1	DSM					89:91	Paenibacillus polymyxa DSM 365	66:95	Paenibacillus polymyxa DSM 365	66:95	Structural elucidation of the fucose containing polysaccharide of Paenibacillus polymyxa DSM 365.
34973768	6	39	theme	Wzy	998:1000	arg1	PepG					1013:1016	the Wzy polymerase PepG	994:1016	the Wzy polymerase PepG	994:1016	Furthermore, we demonstrated that the knock-out of the glycosyltransferases PepQ, PepT, PepU and PepV as well as of the Wzy polymerase PepG led to the absence of paenan II, attributing those enzymes to the assembly of the repeating unit.
34973768	4	40	theme	pure	624:627	arg1	polysaccharide					653:666	a pure minor fucose containing polysaccharide	622:666	a pure minor fucose containing polysaccharide	622:666	Thereby, it was observed that the main polymer fraction was completely suppressed (or deleted) and a pure minor fucose containing polysaccharide could be isolated, which was named paenan II.
34973768	1	41	theme	exopolysaccharides	144:161	arg1	producer					132:139	an avid producer	124:139	an avid producer of exopolysaccharides of industrial interest	124:184	Paenibacillus polymyxa is an avid producer of exopolysaccharides of industrial interest.
34973768	1	41	theme	exopolysaccharides	144:161	arg1	polymyxa					112:119	Paenibacillus polymyxa	98:119	Paenibacillus polymyxa	98:119	Paenibacillus polymyxa is an avid producer of exopolysaccharides of industrial interest.
34973768	0	42	theme	Paenibacillus	66:78	arg1	DSM					89:91	Paenibacillus polymyxa DSM 365	66:95	Paenibacillus polymyxa DSM 365	66:95	Structural elucidation of the fucose containing polysaccharide of Paenibacillus polymyxa DSM 365.
34973768	2	43	theme	structural	246:255	arg1	elucidation					257:267	structural elucidation	246:267	structural elucidation of the polysaccharide	246:289	However, due to the complexity of the polymer composition, structural elucidation of the polysaccharide remained unfeasible for a long time.
32987669	0	0	theme	Coupling	90:97	arg1	Agent					99:103	Coupling Agent	90:103	Coupling Agent	90:103	Hybrid Cellulose-Basalt Polypropylene Composites with Enhanced Compatibility: The Role of Coupling Agent.
32987669	5	1	from	increases	828:836	arg1	modulus					862:868	modulus	862:868	modulus	862:868	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	1	from	increases	828:836	arg1	strength					849:856	tensile strength	841:856	tensile strength	841:856	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	4	2	dep	formulations	736:747	arg1	formulations					736:747	Hybrid formulations	729:747	Hybrid formulations	729:747	Hybrid formulations with basalt/cellulose (15/15) and with 5 wt.
32987669	4	2	dep	formulations	736:747	arg1	wt					790:791	5 wt	788:791	5 wt	788:791	Hybrid formulations with basalt/cellulose (15/15) and with 5 wt.
32987669	4	2	dep	formulations	736:747	arg1	15/15					772:776	15/15	772:776	15/15	772:776	Hybrid formulations with basalt/cellulose (15/15) and with 5 wt.
32987669	4	2	dep	formulations	736:747	arg1	basalt/cellulose					754:769	basalt/cellulose	754:769	basalt/cellulose (15/15)	754:777	Hybrid formulations with basalt/cellulose (15/15) and with 5 wt.
32987669	1	3	theme	PP	265:266	arg1	matrix					269:274	a polypropylene (PP) matrix	248:274	a polypropylene (PP) matrix	248:274	This study deals with the development and optimization of hybrid composites integrating microcrystalline cellulose and short basalt fibers in a polypropylene (PP) matrix to maximize the mechanical properties of resulting composites.
32987669	2	4	theme	acrylic	451:457	arg1	AA-g-PP					465:471	AA-g-PP	465:471	AA-g-PP	465:471	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	2	4	theme	acrylic	451:457	arg1	acid					459:462	acrylic acid	451:462	acrylic acid (AA-g-PP)	451:472	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	7	5	dep	reveal	1193:1198	arg1	compared					1141:1148	compared	1141:1148	compared with the ones available in the literature	1141:1190	The results of this study, if compared with the ones available in the literature, reveal the ability of such a combination of reinforcements to provide materials suitable for automotive applications with environmental benefits.
32987669	2	6	theme	amount	560:565	arg1	effects					356:362	the effects	352:362	the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties	352:517	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	2	6	theme	amount	560:565	arg1	function					542:549	a function	540:549	a function of their amount	540:565	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	1	7	theme	hybrid	164:169	arg1	composites					171:180	hybrid composites	164:180	hybrid composites integrating microcrystalline cellulose and short basalt fibers in a polypropylene (PP) matrix	164:274	This study deals with the development and optimization of hybrid composites integrating microcrystalline cellulose and short basalt fibers in a polypropylene (PP) matrix to maximize the mechanical properties of resulting composites.
32987669	5	8	theme	impact	921:926	arg1	strength					928:935	notched Charpy impact strength	906:935	notched Charpy impact strength	906:935	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	9	from	strength	928:935	arg1	modulus					862:868	modulus	862:868	modulus	862:868	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	9	from	strength	928:935	arg1	strength					849:856	tensile strength	841:856	tensile strength	841:856	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	0	10	theme	Agent	99:103	arg1	Role					82:85	The Role	78:85	Hybrid Cellulose-Basalt Polypropylene Composites with Enhanced Compatibility: The Role of Coupling Agent.	0:104	Hybrid Cellulose-Basalt Polypropylene Composites with Enhanced Compatibility: The Role of Coupling Agent.
32987669	4	11	theme	Hybrid	729:734	arg1	formulations					736:747	Hybrid formulations	729:747	Hybrid formulations	729:747	Hybrid formulations with basalt/cellulose (15/15) and with 5 wt.
32987669	4	11	theme	Hybrid	729:734	arg1	wt					790:791	5 wt	788:791	5 wt	788:791	Hybrid formulations with basalt/cellulose (15/15) and with 5 wt.
32987669	4	11	theme	Hybrid	729:734	arg1	basalt/cellulose					754:769	basalt/cellulose	754:769	basalt/cellulose (15/15)	754:777	Hybrid formulations with basalt/cellulose (15/15) and with 5 wt.
32987669	1	12	dep	development	132:142	arg1	the					128:130	the	128:130	the	128:130	This study deals with the development and optimization of hybrid composites integrating microcrystalline cellulose and short basalt fibers in a polypropylene (PP) matrix to maximize the mechanical properties of resulting composites.
32987669	7	13	from	literature	1181:1190	arg1	available					1164:1172	available	1164:1172	available	1164:1172	The results of this study, if compared with the ones available in the literature, reveal the ability of such a combination of reinforcements to provide materials suitable for automotive applications with environmental benefits.
32987669	5	14	theme	MA-g-PP	799:805	arg1	MA-g-PP					799:805	MA-g-PP	799:805	MA-g-PP	799:805	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	14	theme	MA-g-PP	799:805	arg1	%					794:794	%	794:794	% of MA-g-PP	794:805	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	2	15	with	endowed	398:404	arg1	-PP					442:444	MA-g(grafted)-PP	429:444	MA-g(grafted)-PP	429:444	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	2	15	with	endowed	398:404	arg1	AA-g-PP					465:471	AA-g-PP	465:471	AA-g-PP	465:471	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	2	15	with	endowed	398:404	arg1	acid					459:462	acrylic acid	451:462	acrylic acid (AA-g-PP)	451:472	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	2	15	with	endowed	398:404	arg1	anhydride					418:426	maleic anhydride	411:426	maleic anhydride (MA-g(grafted)-PP)	411:445	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	2	16	theme	maleic	411:416	arg1	-PP					442:444	MA-g(grafted)-PP	429:444	MA-g(grafted)-PP	429:444	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	2	16	theme	maleic	411:416	arg1	anhydride					418:426	maleic anhydride	411:426	maleic anhydride (MA-g(grafted)-PP)	411:445	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	2	17	theme	coupling	381:388	arg1	agents					390:395	two different coupling agents	367:395	two different coupling agents	367:395	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	2	17	theme	coupling	381:388	arg1	functionalities					474:488	endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities	398:488	endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities	398:488	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	1	18	theme	composites	171:180	arg1	optimization					148:159	optimization	148:159	optimization	148:159	This study deals with the development and optimization of hybrid composites integrating microcrystalline cellulose and short basalt fibers in a polypropylene (PP) matrix to maximize the mechanical properties of resulting composites.
32987669	1	18	theme	composites	171:180	arg1	development					132:142	development	132:142	development	132:142	This study deals with the development and optimization of hybrid composites integrating microcrystalline cellulose and short basalt fibers in a polypropylene (PP) matrix to maximize the mechanical properties of resulting composites.
32987669	7	19	theme	automotive	1286:1295	arg1	applications					1297:1308	automotive applications	1286:1308	automotive applications with environmental benefits	1286:1336	The results of this study, if compared with the ones available in the literature, reveal the ability of such a combination of reinforcements to provide materials suitable for automotive applications with environmental benefits.
32987669	2	20	from	effects	356:362	arg1	properties					508:517	the composite properties	494:517	the composite properties	494:517	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	2	21	theme	agents	390:395	arg1	effects					356:362	the effects	352:362	the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties	352:517	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	2	21	theme	agents	390:395	arg1	function					542:549	a function	540:549	a function of their amount	540:565	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	0	22	theme	Cellulose-Basalt	7:22	arg1	Composites					38:47	Hybrid Cellulose-Basalt Polypropylene Composites	0:47	Hybrid Cellulose-Basalt Polypropylene Composites with Enhanced Compatibility: The Role of Coupling Agent.	0:104	Hybrid Cellulose-Basalt Polypropylene Composites with Enhanced Compatibility: The Role of Coupling Agent.
32987669	5	23	theme	neat	997:1000	arg1	PP					1002:1003	neat PP	997:1003	neat PP	997:1003	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	24	from	modulus	893:899	arg1	modulus					862:868	modulus	862:868	modulus	862:868	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	24	from	modulus	893:899	arg1	strength					849:856	tensile strength	841:856	tensile strength	841:856	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	7	25	theme	reinforcements	1237:1250	arg1	combination					1222:1232	such a combination	1215:1232	such a combination of reinforcements	1215:1250	The results of this study, if compared with the ones available in the literature, reveal the ability of such a combination of reinforcements to provide materials suitable for automotive applications with environmental benefits.
32987669	7	26	theme	environmental	1315:1327	arg1	benefits					1329:1336	environmental benefits	1315:1336	environmental benefits	1315:1336	The results of this study, if compared with the ones available in the literature, reveal the ability of such a combination of reinforcements to provide materials suitable for automotive applications with environmental benefits.
32987669	1	27	theme	microcrystalline	194:209	arg1	cellulose					211:219	microcrystalline cellulose	194:219	microcrystalline cellulose	194:219	This study deals with the development and optimization of hybrid composites integrating microcrystalline cellulose and short basalt fibers in a polypropylene (PP) matrix to maximize the mechanical properties of resulting composites.
32987669	0	28	theme	Hybrid	0:5	arg1	Composites					38:47	Hybrid Cellulose-Basalt Polypropylene Composites	0:47	Hybrid Cellulose-Basalt Polypropylene Composites with Enhanced Compatibility: The Role of Coupling Agent.	0:104	Hybrid Cellulose-Basalt Polypropylene Composites with Enhanced Compatibility: The Role of Coupling Agent.
32987669	3	29	dep	lower	648:652	arg1	effectiveness					669:681	effectiveness	669:681	effectiveness	669:681	Tensile, flexural, impact and heat deflection temperature tests highlighted the lower reactivity and effectiveness of AA-g-PP, regardless of reinforcement type.
32987669	3	29	dep	lower	648:652	arg1	reactivity					654:663	reactivity	654:663	reactivity	654:663	Tensile, flexural, impact and heat deflection temperature tests highlighted the lower reactivity and effectiveness of AA-g-PP, regardless of reinforcement type.
32987669	6	30	theme	same	1027:1030	arg1	time					1032:1035	the same time	1023:1035	the same time	1023:1035	At the same time, the thermo-mechanical stability was enhanced by 65% compared to neat PP.
32987669	2	31	theme	different	371:379	arg1	agents					390:395	two different coupling agents	367:395	two different coupling agents	367:395	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	2	31	theme	different	371:379	arg1	functionalities					474:488	endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities	398:488	endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities	398:488	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	2	32	theme	MA-g	429:432	arg1	-PP					442:444	MA-g(grafted)-PP	429:444	MA-g(grafted)-PP	429:444	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	2	32	theme	MA-g	429:432	arg1	anhydride					418:426	maleic anhydride	411:426	maleic anhydride (MA-g(grafted)-PP)	411:445	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	5	33	from	strength	880:887	arg1	modulus					862:868	modulus	862:868	modulus	862:868	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	33	from	strength	880:887	arg1	strength					849:856	tensile strength	841:856	tensile strength	841:856	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	34	theme	notched	906:912	arg1	strength					928:935	notched Charpy impact strength	906:935	notched Charpy impact strength	906:935	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	6	35	theme	neat	1102:1105	arg1	PP					1107:1108	neat PP	1102:1108	neat PP	1102:1108	At the same time, the thermo-mechanical stability was enhanced by 65% compared to neat PP.
32987669	0	36	theme	Polypropylene	24:36	arg1	Composites					38:47	Hybrid Cellulose-Basalt Polypropylene Composites	0:47	Hybrid Cellulose-Basalt Polypropylene Composites with Enhanced Compatibility: The Role of Coupling Agent.	0:104	Hybrid Cellulose-Basalt Polypropylene Composites with Enhanced Compatibility: The Role of Coupling Agent.
32987669	1	37	theme	mechanical	292:301	arg1	properties					303:312	the mechanical properties	288:312	the mechanical properties of resulting composites	288:336	This study deals with the development and optimization of hybrid composites integrating microcrystalline cellulose and short basalt fibers in a polypropylene (PP) matrix to maximize the mechanical properties of resulting composites.
32987669	3	38	theme	heat	598:601	arg1	tests					626:630	Tensile, flexural, impact and heat deflection temperature tests	568:630	Tensile, flexural, impact and heat deflection temperature tests	568:630	Tensile, flexural, impact and heat deflection temperature tests highlighted the lower reactivity and effectiveness of AA-g-PP, regardless of reinforcement type.
32987669	5	39	theme	Charpy	914:919	arg1	strength					928:935	notched Charpy impact strength	906:935	notched Charpy impact strength	906:935	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	1	40	theme	short	225:229	arg1	fibers					238:243	short basalt fibers	225:243	short basalt fibers	225:243	This study deals with the development and optimization of hybrid composites integrating microcrystalline cellulose and short basalt fibers in a polypropylene (PP) matrix to maximize the mechanical properties of resulting composites.
32987669	0	41	theme	Enhanced	54:61	arg1	Compatibility					63:75	Enhanced Compatibility	54:75	Enhanced Compatibility	54:75	Hybrid Cellulose-Basalt Polypropylene Composites with Enhanced Compatibility: The Role of Coupling Agent.
32987669	3	42	theme	reinforcement	709:721	arg1	type					723:726	reinforcement type	709:726	reinforcement type	709:726	Tensile, flexural, impact and heat deflection temperature tests highlighted the lower reactivity and effectiveness of AA-g-PP, regardless of reinforcement type.
32987669	2	43	theme	composite	498:506	arg1	properties					508:517	the composite properties	494:517	the composite properties	494:517	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	3	44	theme	deflection	603:612	arg1	tests					626:630	Tensile, flexural, impact and heat deflection temperature tests	568:630	Tensile, flexural, impact and heat deflection temperature tests	568:630	Tensile, flexural, impact and heat deflection temperature tests highlighted the lower reactivity and effectiveness of AA-g-PP, regardless of reinforcement type.
32987669	5	45	theme	%	952:952	arg1	increases					828:836	remarkable increases	817:836	remarkable increases in tensile strength and modulus	817:868	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	45	theme	%	952:952	arg1	modulus					893:899	modulus	893:899	modulus	893:899	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	45	theme	%	952:952	arg1	strength					928:935	notched Charpy impact strength	906:935	notched Charpy impact strength	906:935	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	45	theme	%	952:952	arg1	strength					880:887	flexural strength	871:887	flexural strength	871:887	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	46	theme	tensile	841:847	arg1	strength					849:856	tensile strength	841:856	tensile strength	841:856	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	1	47	theme	basalt	231:236	arg1	fibers					238:243	short basalt fibers	225:243	short basalt fibers	225:243	This study deals with the development and optimization of hybrid composites integrating microcrystalline cellulose and short basalt fibers in a polypropylene (PP) matrix to maximize the mechanical properties of resulting composites.
32987669	5	48	theme	remarkable	817:826	arg1	increases					828:836	remarkable increases	817:836	remarkable increases in tensile strength and modulus	817:868	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	6	49	theme	thermo-mechanical	1042:1058	arg1	stability					1060:1068	the thermo-mechanical stability	1038:1068	the thermo-mechanical stability	1038:1068	At the same time, the thermo-mechanical stability was enhanced by 65% compared to neat PP.
32987669	3	50	theme	impact	587:592	arg1	tests					626:630	Tensile, flexural, impact and heat deflection temperature tests	568:630	Tensile, flexural, impact and heat deflection temperature tests	568:630	Tensile, flexural, impact and heat deflection temperature tests highlighted the lower reactivity and effectiveness of AA-g-PP, regardless of reinforcement type.
32987669	0	51	with	Composites	38:47	arg1	Compatibility					63:75	Enhanced Compatibility	54:75	Enhanced Compatibility	54:75	Hybrid Cellulose-Basalt Polypropylene Composites with Enhanced Compatibility: The Role of Coupling Agent.
32987669	2	52	theme	endowed	398:404	arg1	agents					390:395	two different coupling agents	367:395	two different coupling agents	367:395	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	2	52	theme	endowed	398:404	arg1	functionalities					474:488	endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities	398:488	endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities	398:488	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	1	53	theme	resulting	317:325	arg1	composites					327:336	resulting composites	317:336	resulting composites	317:336	This study deals with the development and optimization of hybrid composites integrating microcrystalline cellulose and short basalt fibers in a polypropylene (PP) matrix to maximize the mechanical properties of resulting composites.
32987669	5	54	theme	flexural	871:878	arg1	strength					880:887	flexural strength	871:887	flexural strength	871:887	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	1	55	theme	composites	327:336	arg1	properties					303:312	the mechanical properties	288:312	the mechanical properties of resulting composites	288:336	This study deals with the development and optimization of hybrid composites integrating microcrystalline cellulose and short basalt fibers in a polypropylene (PP) matrix to maximize the mechanical properties of resulting composites.
32987669	7	56	theme	available	1164:1172	arg1	ones					1159:1162	the ones	1155:1162	the ones available in the literature	1155:1190	The results of this study, if compared with the ones available in the literature, reveal the ability of such a combination of reinforcements to provide materials suitable for automotive applications with environmental benefits.
32987669	5	57	theme	%	957:957	arg1	increases					828:836	remarkable increases	817:836	remarkable increases in tensile strength and modulus	817:868	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	57	theme	%	957:957	arg1	modulus					893:899	modulus	893:899	modulus	893:899	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	57	theme	%	957:957	arg1	strength					928:935	notched Charpy impact strength	906:935	notched Charpy impact strength	906:935	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	57	theme	%	957:957	arg1	strength					880:887	flexural strength	871:887	flexural strength	871:887	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	7	58	theme	study	1131:1135	arg1	results					1115:1121	The results	1111:1121	The results of this study	1111:1135	The results of this study, if compared with the ones available in the literature, reveal the ability of such a combination of reinforcements to provide materials suitable for automotive applications with environmental benefits.
32987669	5	59	theme	%	975:975	arg1	increases					828:836	remarkable increases	817:836	remarkable increases in tensile strength and modulus	817:868	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	59	theme	%	975:975	arg1	modulus					893:899	modulus	893:899	modulus	893:899	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	59	theme	%	975:975	arg1	strength					928:935	notched Charpy impact strength	906:935	notched Charpy impact strength	906:935	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	59	theme	%	975:975	arg1	strength					880:887	flexural strength	871:887	flexural strength	871:887	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	7	60	with	applications	1297:1308	arg1	benefits					1329:1336	environmental benefits	1315:1336	environmental benefits	1315:1336	The results of this study, if compared with the ones available in the literature, reveal the ability of such a combination of reinforcements to provide materials suitable for automotive applications with environmental benefits.
32987669	7	61	theme	suitable	1273:1280	arg1	materials					1263:1271	materials	1263:1271	materials suitable for automotive applications with environmental benefits	1263:1336	The results of this study, if compared with the ones available in the literature, reveal the ability of such a combination of reinforcements to provide materials suitable for automotive applications with environmental benefits.
32987669	3	62	theme	flexural	577:584	arg1	tests					626:630	Tensile, flexural, impact and heat deflection temperature tests	568:630	Tensile, flexural, impact and heat deflection temperature tests	568:630	Tensile, flexural, impact and heat deflection temperature tests highlighted the lower reactivity and effectiveness of AA-g-PP, regardless of reinforcement type.
32987669	0	63	dep	Composites	38:47	arg1	Role					82:85	The Role	78:85	Hybrid Cellulose-Basalt Polypropylene Composites with Enhanced Compatibility: The Role of Coupling Agent.	0:104	Hybrid Cellulose-Basalt Polypropylene Composites with Enhanced Compatibility: The Role of Coupling Agent.
32987669	7	64	theme	combination	1222:1232	arg1	ability					1204:1210	the ability	1200:1210	the ability of such a combination of reinforcements to provide materials suitable for automotive applications with environmental benefits	1200:1336	The results of this study, if compared with the ones available in the literature, reveal the ability of such a combination of reinforcements to provide materials suitable for automotive applications with environmental benefits.
32987669	3	65	theme	temperature	614:624	arg1	tests					626:630	Tensile, flexural, impact and heat deflection temperature tests	568:630	Tensile, flexural, impact and heat deflection temperature tests	568:630	Tensile, flexural, impact and heat deflection temperature tests highlighted the lower reactivity and effectiveness of AA-g-PP, regardless of reinforcement type.
32987669	5	66	theme	%	943:943	arg1	increases					828:836	remarkable increases	817:836	remarkable increases in tensile strength and modulus	817:868	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	66	theme	%	943:943	arg1	modulus					893:899	modulus	893:899	modulus	893:899	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	66	theme	%	943:943	arg1	strength					928:935	notched Charpy impact strength	906:935	notched Charpy impact strength	906:935	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	5	66	theme	%	943:943	arg1	strength					880:887	flexural strength	871:887	flexural strength	871:887	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	3	67	theme	Tensile	568:574	arg1	tests					626:630	Tensile, flexural, impact and heat deflection temperature tests	568:630	Tensile, flexural, impact and heat deflection temperature tests	568:630	Tensile, flexural, impact and heat deflection temperature tests highlighted the lower reactivity and effectiveness of AA-g-PP, regardless of reinforcement type.
32987669	7	68	from	available	1164:1172	arg1	literature					1181:1190	the literature	1177:1190	the literature	1177:1190	The results of this study, if compared with the ones available in the literature, reveal the ability of such a combination of reinforcements to provide materials suitable for automotive applications with environmental benefits.
32987669	5	69	with	comparison	981:990	arg1	PP					1002:1003	neat PP	997:1003	neat PP	997:1003	% of MA-g-PP displayed remarkable increases in tensile strength and modulus, flexural strength and modulus, and notched Charpy impact strength, of 45% and 284%, 97% and 263%, and 13%, in comparison with neat PP, respectively.
32987669	1	70	theme	polypropylene	250:262	arg1	matrix					269:274	a polypropylene (PP) matrix	248:274	a polypropylene (PP) matrix	248:274	This study deals with the development and optimization of hybrid composites integrating microcrystalline cellulose and short basalt fibers in a polypropylene (PP) matrix to maximize the mechanical properties of resulting composites.
32987669	2	71	theme	grafted	434:440	arg1	-PP					442:444	MA-g(grafted)-PP	429:444	MA-g(grafted)-PP	429:444	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32987669	2	71	theme	grafted	434:440	arg1	anhydride					418:426	maleic anhydride	411:426	maleic anhydride (MA-g(grafted)-PP)	411:445	To this aim, the effects of two different coupling agents, endowed with maleic anhydride (MA-g(grafted)-PP) and acrylic acid (AA-g-PP) functionalities, on the composite properties were investigated as a function of their amount.
32969223	2	0	theme	thiol-ene	516:524	arg1	reaction					526:533	a photoinduced thiol-ene reaction	501:533	a photoinduced thiol-ene reaction	501:533	Cross-linking is achieved by imbibing the composite with a tetrathiol cross-linker and carrying out a photoinduced thiol-ene reaction.
32969223	4	1	theme	E	940:940	arg1	stiff/E					942:948	contrast E'stiff/E'	931:949	contrast E'stiff/E'soft of up 11	931:962	Through cross-linking both the CNCs and matrix, it is possible to access larger wet mechanical contrasts (E'stiff/E'soft = ca. 20) than can be obtained by just cross-linking the matrix alone (where contrast E'stiff/E'soft of up 11 are observed).
32969223	5	2	theme	wt	1029:1030	arg1	P					1076:1076	P	1076:1076	P(VAc-co-VP)	1076:1087	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	5	2	theme	wt	1029:1030	arg1	CNCs					1067:1070	allyl-functionalized tunicate CNCs	1037:1070	allyl-functionalized tunicate CNCs	1037:1070	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	5	2	theme	wt	1029:1030	arg1	VAc-co-VP					1078:1086	VAc-co-VP	1078:1086	VAc-co-VP	1078:1086	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	5	2	theme	wt	1029:1030	arg1	%					1032:1032	15 wt %	1026:1032	15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP)	1026:1087	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	1	3	theme	beak	189:192	arg1	properties					165:174	the water-enhanced mechanical gradient properties	126:174	the water-enhanced mechanical gradient properties of the squid beak	126:192	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	5	4	theme	allyl-functionalized	1037:1056	arg1	CNCs					1067:1070	allyl-functionalized tunicate CNCs	1037:1070	allyl-functionalized tunicate CNCs	1037:1070	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	2	5	theme	photoinduced	503:514	arg1	reaction					526:533	a photoinduced thiol-ene reaction	501:533	a photoinduced thiol-ene reaction	501:533	Cross-linking is achieved by imbibing the composite with a tetrathiol cross-linker and carrying out a photoinduced thiol-ene reaction.
32969223	3	6	theme	wet	687:689	arg1	properties					702:711	the wet mechanical properties	683:711	the wet mechanical properties of these materials	683:730	Central to this study was an investigation on how the placement of cross-links (i.e., within matrix only or between the matrix and filler) impacts the wet mechanical properties of these materials.
32969223	5	7	from	modulus	1163:1169	arg1	MPa					1206:1208	about 14 MPa	1197:1208	about 14 MPa	1197:1208	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	6	8	theme	water	1306:1310	arg1	swelling					1312:1319	The water swelling	1302:1319	The water swelling of the nanocomposites	1302:1341	The water swelling of the nanocomposites is greatly reduced in the cross-linked materials as a result of the thiol-ene cross-linking network, which also contributes to the wet modulus increase.
32969223	6	8	theme	water	1306:1310	arg1	result					1397:1402	a result	1395:1402	a result of the thiol-ene cross-linking network, which also contributes to the wet modulus increase	1395:1493	The water swelling of the nanocomposites is greatly reduced in the cross-linked materials as a result of the thiol-ene cross-linking network, which also contributes to the wet modulus increase.
32969223	3	9	theme	mechanical	691:700	arg1	properties					702:711	the wet mechanical properties	683:711	the wet mechanical properties of these materials	683:730	Central to this study was an investigation on how the placement of cross-links (i.e., within matrix only or between the matrix and filler) impacts the wet mechanical properties of these materials.
32969223	5	10	theme	UV	1286:1287	arg1	irradiation					1289:1299	UV irradiation	1286:1299	UV irradiation	1286:1299	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	1	11	theme	Bioinspired	67:77	arg1	nanocomposites					100:113	Bioinspired cross-linked polymer nanocomposites	67:113	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak	67:192	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	4	12	dep	E	839:839	arg1	20					860:861	20	860:861	20	860:861	Through cross-linking both the CNCs and matrix, it is possible to access larger wet mechanical contrasts (E'stiff/E'soft = ca. 20) than can be obtained by just cross-linking the matrix alone (where contrast E'stiff/E'soft of up 11 are observed).
32969223	4	12	dep	E	839:839	arg1	stiff/E					841:847	stiff/E	841:847	stiff/E	841:847	Through cross-linking both the CNCs and matrix, it is possible to access larger wet mechanical contrasts (E'stiff/E'soft = ca. 20) than can be obtained by just cross-linking the matrix alone (where contrast E'stiff/E'soft of up 11 are observed).
32969223	4	13	theme	contrast	931:938	arg1	stiff/E					942:948	contrast E'stiff/E'	931:949	contrast E'stiff/E'soft of up 11	931:962	Through cross-linking both the CNCs and matrix, it is possible to access larger wet mechanical contrasts (E'stiff/E'soft = ca. 20) than can be obtained by just cross-linking the matrix alone (where contrast E'stiff/E'soft of up 11 are observed).
32969223	4	14	theme	=	854:854	arg1	E					839:839	E	839:839	E'stiff/E'soft =	839:854	Through cross-linking both the CNCs and matrix, it is possible to access larger wet mechanical contrasts (E'stiff/E'soft = ca. 20) than can be obtained by just cross-linking the matrix alone (where contrast E'stiff/E'soft of up 11 are observed).
32969223	7	15	theme	mechanical	1506:1515	arg1	turnability					1517:1527	the mechanical turnability	1502:1527	the mechanical turnability	1502:1527	Given the mechanical turnability and the relatively simple approach that also allows photopatterning the material properties, these water-activated bioinspired nanocomposites have potential uses in a broad range of biomedical applications, such as mechanically compliant intracortical microelectrodes.
32969223	1	16	theme	cross-linked	79:90	arg1	nanocomposites					100:113	Bioinspired cross-linked polymer nanocomposites	67:113	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak	67:192	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	7	17	theme	simple	1548:1553	arg1	approach					1555:1562	the relatively simple approach	1533:1562	the relatively simple approach that also allows photopatterning the material properties	1533:1619	Given the mechanical turnability and the relatively simple approach that also allows photopatterning the material properties, these water-activated bioinspired nanocomposites have potential uses in a broad range of biomedical applications, such as mechanically compliant intracortical microelectrodes.
32969223	4	18	dep	mechanical	817:826	arg1	E					839:839	E	839:839	E'stiff/E'soft =	839:854	Through cross-linking both the CNCs and matrix, it is possible to access larger wet mechanical contrasts (E'stiff/E'soft = ca. 20) than can be obtained by just cross-linking the matrix alone (where contrast E'stiff/E'soft of up 11 are observed).
32969223	4	18	dep	mechanical	817:826	arg1	contrasts					828:836	contrasts	828:836	contrasts	828:836	Through cross-linking both the CNCs and matrix, it is possible to access larger wet mechanical contrasts (E'stiff/E'soft = ca. 20) than can be obtained by just cross-linking the matrix alone (where contrast E'stiff/E'soft of up 11 are observed).
32969223	1	19	theme	polymer	92:98	arg1	nanocomposites					100:113	Bioinspired cross-linked polymer nanocomposites	67:113	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak	67:192	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	4	20	dep	20	860:861	arg1	ca.					856:858	ca.	856:858	ca.	856:858	Through cross-linking both the CNCs and matrix, it is possible to access larger wet mechanical contrasts (E'stiff/E'soft = ca. 20) than can be obtained by just cross-linking the matrix alone (where contrast E'stiff/E'soft of up 11 are observed).
32969223	1	21	link	cross-linked	79:90	arg1	nanocomposites					100:113	Bioinspired cross-linked polymer nanocomposites	67:113	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak	67:192	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	2	22	with	composite	443:451	arg1	cross-linker					471:482	a tetrathiol cross-linker	458:482	a tetrathiol cross-linker	458:482	Cross-linking is achieved by imbibing the composite with a tetrathiol cross-linker and carrying out a photoinduced thiol-ene reaction.
32969223	4	23	theme	up	958:959	arg1	soft					950:953	soft	950:953	soft	950:953	Through cross-linking both the CNCs and matrix, it is possible to access larger wet mechanical contrasts (E'stiff/E'soft = ca. 20) than can be obtained by just cross-linking the matrix alone (where contrast E'stiff/E'soft of up 11 are observed).
32969223	6	24	theme	wet	1474:1476	arg1	increase					1486:1493	the wet modulus increase	1470:1493	the wet modulus increase	1470:1493	The water swelling of the nanocomposites is greatly reduced in the cross-linked materials as a result of the thiol-ene cross-linking network, which also contributes to the wet modulus increase.
32969223	2	25	theme	tetrathiol	460:469	arg1	cross-linker					471:482	a tetrathiol cross-linker	458:482	a tetrathiol cross-linker	458:482	Cross-linking is achieved by imbibing the composite with a tetrathiol cross-linker and carrying out a photoinduced thiol-ene reaction.
32969223	5	26	theme	P	1076:1076	arg1	P					1076:1076	P	1076:1076	P(VAc-co-VP)	1076:1087	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	5	26	theme	P	1076:1076	arg1	CNCs					1067:1070	allyl-functionalized tunicate CNCs	1037:1070	allyl-functionalized tunicate CNCs	1037:1070	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	5	26	theme	P	1076:1076	arg1	VAc-co-VP					1078:1086	VAc-co-VP	1078:1086	VAc-co-VP	1078:1086	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	5	26	theme	P	1076:1076	arg1	%					1032:1032	15 wt %	1026:1032	15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP)	1026:1087	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	0	27	theme	Beak	6:9	arg1	Composites					55:64	Squid Beak Inspired Cross-Linked Cellulose Nanocrystal Composites	0:64	Squid Beak Inspired Cross-Linked Cellulose Nanocrystal Composites	0:64	Squid Beak Inspired Cross-Linked Cellulose Nanocrystal Composites.
32969223	3	28	theme	materials	722:730	arg1	properties					702:711	the wet mechanical properties	683:711	the wet mechanical properties of these materials	683:730	Central to this study was an investigation on how the placement of cross-links (i.e., within matrix only or between the matrix and filler) impacts the wet mechanical properties of these materials.
32969223	6	29	link	cross-linked	1369:1380	arg1	materials					1382:1390	the cross-linked materials	1365:1390	the cross-linked materials	1365:1390	The water swelling of the nanocomposites is greatly reduced in the cross-linked materials as a result of the thiol-ene cross-linking network, which also contributes to the wet modulus increase.
32969223	7	30	theme	intracortical	1767:1779	arg1	microelectrodes					1781:1795	mechanically compliant intracortical microelectrodes	1744:1795	mechanically compliant intracortical microelectrodes	1744:1795	Given the mechanical turnability and the relatively simple approach that also allows photopatterning the material properties, these water-activated bioinspired nanocomposites have potential uses in a broad range of biomedical applications, such as mechanically compliant intracortical microelectrodes.
32969223	0	31	theme	Squid	0:4	arg1	Composites					55:64	Squid Beak Inspired Cross-Linked Cellulose Nanocrystal Composites	0:64	Squid Beak Inspired Cross-Linked Cellulose Nanocrystal Composites	0:64	Squid Beak Inspired Cross-Linked Cellulose Nanocrystal Composites.
32969223	1	32	theme	alkene-containing	311:327	arg1	matrix					337:342	an alkene-containing polymer matrix	308:342	an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP))	308:398	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	5	33	theme	wet	1178:1180	arg1	composite					1182:1190	the wet composite	1174:1190	the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C)	1174:1262	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	0	34	theme	Cross-Linked	20:31	arg1	Composites					55:64	Squid Beak Inspired Cross-Linked Cellulose Nanocrystal Composites	0:64	Squid Beak Inspired Cross-Linked Cellulose Nanocrystal Composites	0:64	Squid Beak Inspired Cross-Linked Cellulose Nanocrystal Composites.
32969223	1	35	theme	polymer	329:335	arg1	matrix					337:342	an alkene-containing polymer matrix	308:342	an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP))	308:398	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	6	36	theme	modulus	1478:1484	arg1	increase					1486:1493	the wet modulus increase	1470:1493	the wet modulus increase	1470:1493	The water swelling of the nanocomposites is greatly reduced in the cross-linked materials as a result of the thiol-ene cross-linking network, which also contributes to the wet modulus increase.
32969223	5	37	theme	composite	1182:1190	arg1	modulus					1163:1169	the modulus	1159:1169	the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C)	1159:1262	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	0	38	theme	Inspired	11:18	arg1	Composites					55:64	Squid Beak Inspired Cross-Linked Cellulose Nanocrystal Composites	0:64	Squid Beak Inspired Cross-Linked Cellulose Nanocrystal Composites	0:64	Squid Beak Inspired Cross-Linked Cellulose Nanocrystal Composites.
32969223	7	39	theme	broad	1696:1700	arg1	range					1702:1706	a broad range	1694:1706	a broad range of biomedical applications, such as mechanically compliant intracortical microelectrodes	1694:1795	Given the mechanical turnability and the relatively simple approach that also allows photopatterning the material properties, these water-activated bioinspired nanocomposites have potential uses in a broad range of biomedical applications, such as mechanically compliant intracortical microelectrodes.
32969223	7	39	theme	broad	1696:1700	arg1	microelectrodes					1781:1795	mechanically compliant intracortical microelectrodes	1744:1795	mechanically compliant intracortical microelectrodes	1744:1795	Given the mechanical turnability and the relatively simple approach that also allows photopatterning the material properties, these water-activated bioinspired nanocomposites have potential uses in a broad range of biomedical applications, such as mechanically compliant intracortical microelectrodes.
32969223	4	40	dep	matrix	911:916	arg1	observed					968:975	observed	968:975	are observed	964:975	Through cross-linking both the CNCs and matrix, it is possible to access larger wet mechanical contrasts (E'stiff/E'soft = ca. 20) than can be obtained by just cross-linking the matrix alone (where contrast E'stiff/E'soft of up 11 are observed).
32969223	1	41	theme	water-enhanced	130:143	arg1	properties					165:174	the water-enhanced mechanical gradient properties	126:174	the water-enhanced mechanical gradient properties of the squid beak	126:192	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	1	42	theme	carboxylic	233:242	arg1	acid-					244:248	carboxylic acid-	233:248	carboxylic acid-	233:248	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	0	43	theme	Nanocrystal	43:53	arg1	Composites					55:64	Squid Beak Inspired Cross-Linked Cellulose Nanocrystal Composites	0:64	Squid Beak Inspired Cross-Linked Cellulose Nanocrystal Composites	0:64	Squid Beak Inspired Cross-Linked Cellulose Nanocrystal Composites.
32969223	5	44	from	MPa	1206:1208	arg1	modulus					1163:1169	the modulus	1159:1169	the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C)	1159:1262	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	5	44	from	MPa	1206:1208	arg1	composite					1182:1190	the wet composite	1174:1190	the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C)	1174:1262	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	5	45	from	temperature	1244:1254	arg1	MPa					1223:1225	about 289 MPa	1213:1225	about 289 MPa at physiological temperature (37 °C)	1213:1262	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	0	46	theme	Cellulose	33:41	arg1	Composites					55:64	Squid Beak Inspired Cross-Linked Cellulose Nanocrystal Composites	0:64	Squid Beak Inspired Cross-Linked Cellulose Nanocrystal Composites	0:64	Squid Beak Inspired Cross-Linked Cellulose Nanocrystal Composites.
32969223	5	47	theme	CNCs	1067:1070	arg1	P					1076:1076	P	1076:1076	P(VAc-co-VP)	1076:1087	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	5	47	theme	CNCs	1067:1070	arg1	CNCs					1067:1070	allyl-functionalized tunicate CNCs	1037:1070	allyl-functionalized tunicate CNCs	1037:1070	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	5	47	theme	CNCs	1067:1070	arg1	VAc-co-VP					1078:1086	VAc-co-VP	1078:1086	VAc-co-VP	1078:1086	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	5	47	theme	CNCs	1067:1070	arg1	%					1032:1032	15 wt %	1026:1032	15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP)	1026:1087	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	7	48	contain	have	1671:1674	arg2	potential					1676:1684	potential uses	1676:1689	potential uses	1676:1689	Given the mechanical turnability and the relatively simple approach that also allows photopatterning the material properties, these water-activated bioinspired nanocomposites have potential uses in a broad range of biomedical applications, such as mechanically compliant intracortical microelectrodes.
32969223	7	48	contain	have	1671:1674	arg1	nanocomposites					1656:1669	these water-activated bioinspired nanocomposites	1622:1669	these water-activated bioinspired nanocomposites	1622:1669	Given the mechanical turnability and the relatively simple approach that also allows photopatterning the material properties, these water-activated bioinspired nanocomposites have potential uses in a broad range of biomedical applications, such as mechanically compliant intracortical microelectrodes.
32969223	4	49	theme	larger	806:811	arg1	access					799:804	access	799:804	access larger wet mechanical contrasts (E'stiff/E'soft = ca. 20) than can be obtained by just cross-linking the matrix alone (where contrast E'stiff/E'soft of up 11 are observed)	799:976	Through cross-linking both the CNCs and matrix, it is possible to access larger wet mechanical contrasts (E'stiff/E'soft = ca. 20) than can be obtained by just cross-linking the matrix alone (where contrast E'stiff/E'soft of up 11 are observed).
32969223	4	50	dep	larger	806:811	arg1	mechanical					817:826	mechanical	817:826	mechanical	817:826	Through cross-linking both the CNCs and matrix, it is possible to access larger wet mechanical contrasts (E'stiff/E'soft = ca. 20) than can be obtained by just cross-linking the matrix alone (where contrast E'stiff/E'soft of up 11 are observed).
32969223	4	51	theme	soft	950:953	arg1	stiff/E					942:948	contrast E'stiff/E'	931:949	contrast E'stiff/E'soft of up 11	931:962	Through cross-linking both the CNCs and matrix, it is possible to access larger wet mechanical contrasts (E'stiff/E'soft = ca. 20) than can be obtained by just cross-linking the matrix alone (where contrast E'stiff/E'soft of up 11 are observed).
32969223	7	52	theme	compliant	1757:1765	arg1	microelectrodes					1781:1795	mechanically compliant intracortical microelectrodes	1744:1795	mechanically compliant intracortical microelectrodes	1744:1795	Given the mechanical turnability and the relatively simple approach that also allows photopatterning the material properties, these water-activated bioinspired nanocomposites have potential uses in a broad range of biomedical applications, such as mechanically compliant intracortical microelectrodes.
32969223	3	53	theme	cross-links	603:613	arg1	placement					590:598	the placement	586:598	the placement of cross-links (i.e., within matrix only or between the matrix and filler)	586:673	Central to this study was an investigation on how the placement of cross-links (i.e., within matrix only or between the matrix and filler) impacts the wet mechanical properties of these materials.
32969223	1	54	theme	mechanical	145:154	arg1	properties					165:174	the water-enhanced mechanical gradient properties	126:174	the water-enhanced mechanical gradient properties of the squid beak	126:192	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	5	55	theme	tunicate	1058:1065	arg1	CNCs					1067:1070	allyl-functionalized tunicate CNCs	1037:1070	allyl-functionalized tunicate CNCs	1037:1070	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	1	56	theme	vinyl	350:354	arg1	pentenoate					373:382	vinyl acetate-co-vinyl pentenoate	350:382	vinyl acetate-co-vinyl pentenoate	350:382	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	1	56	theme	vinyl	350:354	arg1	poly					345:348	poly	345:348	poly(vinyl acetate-co-vinyl pentenoate)	345:383	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	6	57	theme	cross-linked	1369:1380	arg1	materials					1382:1390	the cross-linked materials	1365:1390	the cross-linked materials	1365:1390	The water swelling of the nanocomposites is greatly reduced in the cross-linked materials as a result of the thiol-ene cross-linking network, which also contributes to the wet modulus increase.
32969223	5	58	dep	%	1107:1107	arg1	mol					1103:1105	mol	1103:1105	mol	1103:1105	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	7	59	theme	biomedical	1711:1720	arg1	applications					1722:1733	biomedical applications	1711:1733	biomedical applications	1711:1733	Given the mechanical turnability and the relatively simple approach that also allows photopatterning the material properties, these water-activated bioinspired nanocomposites have potential uses in a broad range of biomedical applications, such as mechanically compliant intracortical microelectrodes.
32969223	7	59	theme	biomedical	1711:1720	arg1	microelectrodes					1781:1795	mechanically compliant intracortical microelectrodes	1744:1795	mechanically compliant intracortical microelectrodes	1744:1795	Given the mechanical turnability and the relatively simple approach that also allows photopatterning the material properties, these water-activated bioinspired nanocomposites have potential uses in a broad range of biomedical applications, such as mechanically compliant intracortical microelectrodes.
32969223	1	60	theme	gradient	156:163	arg1	properties					165:174	the water-enhanced mechanical gradient properties	126:174	the water-enhanced mechanical gradient properties of the squid beak	126:192	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	1	61	theme	acetate-co-vinyl	356:371	arg1	pentenoate					373:382	vinyl acetate-co-vinyl pentenoate	350:382	vinyl acetate-co-vinyl pentenoate	350:382	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	1	61	theme	acetate-co-vinyl	356:371	arg1	poly					345:348	poly	345:348	poly(vinyl acetate-co-vinyl pentenoate)	345:383	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	7	62	theme	bioinspired	1644:1654	arg1	nanocomposites					1656:1669	these water-activated bioinspired nanocomposites	1622:1669	these water-activated bioinspired nanocomposites	1622:1669	Given the mechanical turnability and the relatively simple approach that also allows photopatterning the material properties, these water-activated bioinspired nanocomposites have potential uses in a broad range of biomedical applications, such as mechanically compliant intracortical microelectrodes.
32969223	7	63	theme	water-activated	1628:1642	arg1	nanocomposites					1656:1669	these water-activated bioinspired nanocomposites	1622:1669	these water-activated bioinspired nanocomposites	1622:1669	Given the mechanical turnability and the relatively simple approach that also allows photopatterning the material properties, these water-activated bioinspired nanocomposites have potential uses in a broad range of biomedical applications, such as mechanically compliant intracortical microelectrodes.
32969223	6	64	theme	network	1435:1441	arg1	swelling					1312:1319	The water swelling	1302:1319	The water swelling of the nanocomposites	1302:1341	The water swelling of the nanocomposites is greatly reduced in the cross-linked materials as a result of the thiol-ene cross-linking network, which also contributes to the wet modulus increase.
32969223	6	64	theme	network	1435:1441	arg1	result					1397:1402	a result	1395:1402	a result of the thiol-ene cross-linking network, which also contributes to the wet modulus increase	1395:1493	The water swelling of the nanocomposites is greatly reduced in the cross-linked materials as a result of the thiol-ene cross-linking network, which also contributes to the wet modulus increase.
32969223	5	65	theme	physiological	1230:1242	arg1	temperature					1244:1254	physiological temperature	1230:1254	physiological temperature (37 °C)	1230:1262	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	5	65	theme	physiological	1230:1242	arg1	°C					1260:1261	37 °C	1257:1261	37 °C	1257:1261	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	7	66	dep	potential	1676:1684	arg1	uses					1686:1689	uses	1686:1689	uses	1686:1689	Given the mechanical turnability and the relatively simple approach that also allows photopatterning the material properties, these water-activated bioinspired nanocomposites have potential uses in a broad range of biomedical applications, such as mechanically compliant intracortical microelectrodes.
32969223	1	67	theme	allyl-functionalized	253:272	arg1	nanocrystals					284:295	allyl-functionalized cellulose nanocrystals	253:295	allyl-functionalized cellulose nanocrystals (CNC)	253:301	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	1	67	theme	allyl-functionalized	253:272	arg1	CNC					298:300	CNC	298:300	CNC	298:300	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	7	68	theme	applications	1722:1733	arg1	range					1702:1706	a broad range	1694:1706	a broad range of biomedical applications, such as mechanically compliant intracortical microelectrodes	1694:1795	Given the mechanical turnability and the relatively simple approach that also allows photopatterning the material properties, these water-activated bioinspired nanocomposites have potential uses in a broad range of biomedical applications, such as mechanically compliant intracortical microelectrodes.
32969223	7	68	theme	applications	1722:1733	arg1	microelectrodes					1781:1795	mechanically compliant intracortical microelectrodes	1744:1795	mechanically compliant intracortical microelectrodes	1744:1795	Given the mechanical turnability and the relatively simple approach that also allows photopatterning the material properties, these water-activated bioinspired nanocomposites have potential uses in a broad range of biomedical applications, such as mechanically compliant intracortical microelectrodes.
32969223	5	69	from	increase	1147:1154	arg1	modulus					1163:1169	the modulus	1159:1169	the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C)	1159:1262	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	6	70	theme	cross-linking	1421:1433	arg1	network					1435:1441	the thiol-ene cross-linking network	1407:1441	the thiol-ene cross-linking network	1407:1441	The water swelling of the nanocomposites is greatly reduced in the cross-linked materials as a result of the thiol-ene cross-linking network, which also contributes to the wet modulus increase.
32969223	4	71	dep	cross-linking	741:753	arg1	both					755:758	both	755:758	both	755:758	Through cross-linking both the CNCs and matrix, it is possible to access larger wet mechanical contrasts (E'stiff/E'soft = ca. 20) than can be obtained by just cross-linking the matrix alone (where contrast E'stiff/E'soft of up 11 are observed).
32969223	6	72	theme	nanocomposites	1328:1341	arg1	swelling					1312:1319	The water swelling	1302:1319	The water swelling of the nanocomposites	1302:1341	The water swelling of the nanocomposites is greatly reduced in the cross-linked materials as a result of the thiol-ene cross-linking network, which also contributes to the wet modulus increase.
32969223	6	72	theme	nanocomposites	1328:1341	arg1	result					1397:1402	a result	1395:1402	a result of the thiol-ene cross-linking network, which also contributes to the wet modulus increase	1395:1493	The water swelling of the nanocomposites is greatly reduced in the cross-linked materials as a result of the thiol-ene cross-linking network, which also contributes to the wet modulus increase.
32969223	1	73	theme	cellulose	274:282	arg1	nanocrystals					284:295	allyl-functionalized cellulose nanocrystals	253:295	allyl-functionalized cellulose nanocrystals (CNC)	253:301	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	1	73	theme	cellulose	274:282	arg1	CNC					298:300	CNC	298:300	CNC	298:300	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	5	74	dep	mol	1103:1105	arg1	30					1100:1101	30	1100:1101	30	1100:1101	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	6	75	theme	thiol-ene	1411:1419	arg1	network					1435:1441	the thiol-ene cross-linking network	1407:1441	the thiol-ene cross-linking network	1407:1441	The water swelling of the nanocomposites is greatly reduced in the cross-linked materials as a result of the thiol-ene cross-linking network, which also contributes to the wet modulus increase.
32969223	7	76	theme	material	1601:1608	arg1	properties					1610:1619	the material properties	1597:1619	the material properties	1597:1619	Given the mechanical turnability and the relatively simple approach that also allows photopatterning the material properties, these water-activated bioinspired nanocomposites have potential uses in a broad range of biomedical applications, such as mechanically compliant intracortical microelectrodes.
32969223	5	77	theme	VP	1134:1135	arg1	units					1137:1141	the alkene-containing VP units	1112:1141	the alkene-containing VP units	1112:1141	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	5	78	located	observed	1271:1278	arg2	increase					1147:1154	an increase	1144:1154	an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C)	1144:1262	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	5	78	located	observed	1271:1278	arg1	nanocomposites					995:1008	nanocomposites	995:1008	nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units	995:1141	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	1	79	dep	matrix	337:342	arg1	pentenoate					373:382	vinyl acetate-co-vinyl pentenoate	350:382	vinyl acetate-co-vinyl pentenoate	350:382	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	1	79	dep	matrix	337:342	arg1	P					386:386	P(VAc-co-VP)	386:397	P(VAc-co-VP)	386:397	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	1	79	dep	matrix	337:342	arg1	poly					345:348	poly	345:348	poly(vinyl acetate-co-vinyl pentenoate)	345:383	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	5	80	theme	alkene-containing	1116:1132	arg1	units					1137:1141	the alkene-containing VP units	1112:1141	the alkene-containing VP units	1112:1141	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	1	81	theme	squid	183:187	arg1	beak					189:192	the squid beak	179:192	the squid beak	179:192	Bioinspired cross-linked polymer nanocomposites that mimic the water-enhanced mechanical gradient properties of the squid beak have been prepared by embedding either carboxylic acid- or allyl-functionalized cellulose nanocrystals (CNC) into an alkene-containing polymer matrix (poly(vinyl acetate-co-vinyl pentenoate), P(VAc-co-VP)).
32969223	5	82	theme	units	1137:1141	arg1	%					1107:1107	about 30 mol %	1094:1107	about 30 mol % of the alkene-containing VP units	1094:1141	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
32969223	5	82	theme	units	1137:1141	arg1	units					1137:1141	the alkene-containing VP units	1112:1141	the alkene-containing VP units	1112:1141	For example, in nanocomposites fabricated with 15 wt % of allyl-functionalized tunicate CNCs and P(VAc-co-VP) with about 30 mol % of the alkene-containing VP units, an increase in the modulus of the wet composite from about 14 MPa to about 289 MPa at physiological temperature (37 °C) can be observed after UV irradiation.
33373794	0	0	theme	enhanced	111:118	arg1	separation					130:139	enhanced selective separation	111:139	enhanced selective separation of histidine-rich proteins	111:166	Preparation and characterization of highly porous cellulose-agarose composite chromatographic microspheres for enhanced selective separation of histidine-rich proteins.
33373794	4	1	from	morphology	693:702	arg1	structure					664:672	The micro-meso-macroporous structure	637:672	The micro-meso-macroporous structure of microspheres in morphology	637:702	The micro-meso-macroporous structure of microspheres in morphology is conducive to high permeability and adsorption capacity, and the microspheres possess high specific surface area of 183.81 m2/g.
33373794	4	1	from	morphology	693:702	arg1	conducive					707:715	conducive	707:715	conducive	707:715	The micro-meso-macroporous structure of microspheres in morphology is conducive to high permeability and adsorption capacity, and the microspheres possess high specific surface area of 183.81 m2/g.
33373794	5	2	theme	adsorption	932:941	arg1	capacity					943:950	high adsorption capacity	927:950	high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb)	927:993	The affinity chromatographic microspheres are prepared by immobilizing Cu2+, which exhibits high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb), fast adsorption rate wihin 40 minutes, well-selectivity, and excellent recyclability in ten cycles.
33373794	4	3	theme	high	792:795	arg1	area					814:817	high specific surface area	792:817	high specific surface area of 183.81 m2/g	792:832	The micro-meso-macroporous structure of microspheres in morphology is conducive to high permeability and adsorption capacity, and the microspheres possess high specific surface area of 183.81 m2/g.
33373794	5	4	from	recyclability	1067:1079	arg1	cycles					1088:1093	ten cycles	1084:1093	ten cycles	1084:1093	The affinity chromatographic microspheres are prepared by immobilizing Cu2+, which exhibits high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb), fast adsorption rate wihin 40 minutes, well-selectivity, and excellent recyclability in ten cycles.
33373794	1	5	theme	porous	187:192	arg1	microspheres					212:223	the porous cellulose-agarose microspheres	183:223	the porous cellulose-agarose microspheres with high specific surface area and enhanced mechanical strength	183:288	In this work, the porous cellulose-agarose microspheres with high specific surface area and enhanced mechanical strength are prepared by a novel chemical crosslinking method.
33373794	6	6	theme	purification	1203:1214	arg1	performance					1170:1180	the high performance	1161:1180	the high performance of biomacromolecular purification	1161:1214	We expect that this work to provide an outstanding candidate for the high performance of biomacromolecular purification.
33373794	4	7	theme	micro-meso-macroporous	641:662	arg1	structure					664:672	The micro-meso-macroporous structure	637:672	The micro-meso-macroporous structure of microspheres in morphology	637:702	The micro-meso-macroporous structure of microspheres in morphology is conducive to high permeability and adsorption capacity, and the microspheres possess high specific surface area of 183.81 m2/g.
33373794	4	7	theme	micro-meso-macroporous	641:662	arg1	conducive					707:715	conducive	707:715	conducive	707:715	The micro-meso-macroporous structure of microspheres in morphology is conducive to high permeability and adsorption capacity, and the microspheres possess high specific surface area of 183.81 m2/g.
33373794	4	8	from	microspheres	677:688	arg1	morphology					693:702	morphology	693:702	morphology	693:702	The micro-meso-macroporous structure of microspheres in morphology is conducive to high permeability and adsorption capacity, and the microspheres possess high specific surface area of 183.81 m2/g.
33373794	5	9	theme	immobilizing	893:904	arg1	Cu2+					906:909	immobilizing Cu2+	893:909	immobilizing Cu2+	893:909	The affinity chromatographic microspheres are prepared by immobilizing Cu2+, which exhibits high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb), fast adsorption rate wihin 40 minutes, well-selectivity, and excellent recyclability in ten cycles.
33373794	3	10	theme	regions	628:634	arg1	content					607:613	high content	602:613	high content of amorphous regions	602:634	The prepared microspheres exhibit low crystallinity of 12.45%, which means high content of amorphous regions.
33373794	4	11	theme	microspheres	677:688	arg1	structure					664:672	The micro-meso-macroporous structure	637:672	The micro-meso-macroporous structure of microspheres in morphology	637:702	The micro-meso-macroporous structure of microspheres in morphology is conducive to high permeability and adsorption capacity, and the microspheres possess high specific surface area of 183.81 m2/g.
33373794	4	11	theme	microspheres	677:688	arg1	conducive					707:715	conducive	707:715	conducive	707:715	The micro-meso-macroporous structure of microspheres in morphology is conducive to high permeability and adsorption capacity, and the microspheres possess high specific surface area of 183.81 m2/g.
33373794	0	12	theme	selective	120:128	arg1	separation					130:139	enhanced selective separation	111:139	enhanced selective separation of histidine-rich proteins	111:166	Preparation and characterization of highly porous cellulose-agarose composite chromatographic microspheres for enhanced selective separation of histidine-rich proteins.
33373794	4	13	from	structure	664:672	arg1	morphology					693:702	morphology	693:702	morphology	693:702	The micro-meso-macroporous structure of microspheres in morphology is conducive to high permeability and adsorption capacity, and the microspheres possess high specific surface area of 183.81 m2/g.
33373794	1	14	theme	novel	308:312	arg1	method					336:341	a novel chemical crosslinking method	306:341	a novel chemical crosslinking method	306:341	In this work, the porous cellulose-agarose microspheres with high specific surface area and enhanced mechanical strength are prepared by a novel chemical crosslinking method.
33373794	0	15	theme	histidine-rich	144:157	arg1	proteins					159:166	histidine-rich proteins	144:166	histidine-rich proteins	144:166	Preparation and characterization of highly porous cellulose-agarose composite chromatographic microspheres for enhanced selective separation of histidine-rich proteins.
33373794	5	16	theme	fast	996:999	arg1	rate					1012:1015	fast adsorption rate	996:1015	fast adsorption rate wihin 40 minutes	996:1032	The affinity chromatographic microspheres are prepared by immobilizing Cu2+, which exhibits high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb), fast adsorption rate wihin 40 minutes, well-selectivity, and excellent recyclability in ten cycles.
33373794	2	17	theme	inherent	479:486	arg1	network					505:511	the inherent hydrogen bonding network	475:511	the inherent hydrogen bonding network of cellulose	475:524	The crosslinking reaction homogeneously proceeds between polysaccharides, and the covalent bonding network is generated to replace the inherent hydrogen bonding network of cellulose.
33373794	5	18	theme	excellent	1057:1065	arg1	recyclability					1067:1079	excellent recyclability	1057:1079	excellent recyclability in ten cycles	1057:1093	The affinity chromatographic microspheres are prepared by immobilizing Cu2+, which exhibits high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb), fast adsorption rate wihin 40 minutes, well-selectivity, and excellent recyclability in ten cycles.
33373794	1	19	theme	cellulose-agarose	194:210	arg1	microspheres					212:223	the porous cellulose-agarose microspheres	183:223	the porous cellulose-agarose microspheres with high specific surface area and enhanced mechanical strength	183:288	In this work, the porous cellulose-agarose microspheres with high specific surface area and enhanced mechanical strength are prepared by a novel chemical crosslinking method.
33373794	3	20	theme	prepared	531:538	arg1	microspheres					540:551	The prepared microspheres	527:551	The prepared microspheres	527:551	The prepared microspheres exhibit low crystallinity of 12.45%, which means high content of amorphous regions.
33373794	5	21	from	rate	1012:1015	arg1	cycles					1088:1093	ten cycles	1084:1093	ten cycles	1084:1093	The affinity chromatographic microspheres are prepared by immobilizing Cu2+, which exhibits high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb), fast adsorption rate wihin 40 minutes, well-selectivity, and excellent recyclability in ten cycles.
33373794	3	22	theme	high	602:605	arg1	content					607:613	high content	602:613	high content of amorphous regions	602:634	The prepared microspheres exhibit low crystallinity of 12.45%, which means high content of amorphous regions.
33373794	6	23	theme	high	1165:1168	arg1	performance					1170:1180	the high performance	1161:1180	the high performance of biomacromolecular purification	1161:1214	We expect that this work to provide an outstanding candidate for the high performance of biomacromolecular purification.
33373794	4	24	theme	adsorption	742:751	arg1	capacity					753:760	adsorption capacity	742:760	adsorption capacity	742:760	The micro-meso-macroporous structure of microspheres in morphology is conducive to high permeability and adsorption capacity, and the microspheres possess high specific surface area of 183.81 m2/g.
33373794	2	25	theme	cellulose	516:524	arg1	network					505:511	the inherent hydrogen bonding network	475:511	the inherent hydrogen bonding network of cellulose	475:524	The crosslinking reaction homogeneously proceeds between polysaccharides, and the covalent bonding network is generated to replace the inherent hydrogen bonding network of cellulose.
33373794	5	26	theme	affinity	839:846	arg1	microspheres					864:875	The affinity chromatographic microspheres	835:875	The affinity chromatographic microspheres	835:875	The affinity chromatographic microspheres are prepared by immobilizing Cu2+, which exhibits high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb), fast adsorption rate wihin 40 minutes, well-selectivity, and excellent recyclability in ten cycles.
33373794	4	27	theme	m2/g	829:832	arg1	area					814:817	high specific surface area	792:817	high specific surface area of 183.81 m2/g	792:832	The micro-meso-macroporous structure of microspheres in morphology is conducive to high permeability and adsorption capacity, and the microspheres possess high specific surface area of 183.81 m2/g.
33373794	5	28	theme	bovine	971:976	arg1	BHb					990:992	BHb	990:992	BHb	990:992	The affinity chromatographic microspheres are prepared by immobilizing Cu2+, which exhibits high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb), fast adsorption rate wihin 40 minutes, well-selectivity, and excellent recyclability in ten cycles.
33373794	5	28	theme	bovine	971:976	arg1	hemoglobin					978:987	bovine hemoglobin	971:987	bovine hemoglobin (BHb)	971:993	The affinity chromatographic microspheres are prepared by immobilizing Cu2+, which exhibits high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb), fast adsorption rate wihin 40 minutes, well-selectivity, and excellent recyclability in ten cycles.
33373794	1	29	theme	chemical	314:321	arg1	method					336:341	a novel chemical crosslinking method	306:341	a novel chemical crosslinking method	306:341	In this work, the porous cellulose-agarose microspheres with high specific surface area and enhanced mechanical strength are prepared by a novel chemical crosslinking method.
33373794	1	30	theme	high	230:233	arg1	area					252:255	high specific surface area	230:255	high specific surface area	230:255	In this work, the porous cellulose-agarose microspheres with high specific surface area and enhanced mechanical strength are prepared by a novel chemical crosslinking method.
33373794	1	31	theme	crosslinking	323:334	arg1	method					336:341	a novel chemical crosslinking method	306:341	a novel chemical crosslinking method	306:341	In this work, the porous cellulose-agarose microspheres with high specific surface area and enhanced mechanical strength are prepared by a novel chemical crosslinking method.
33373794	5	32	theme	wihin	1017:1021	arg1	minutes					1026:1032	wihin 40 minutes	1017:1032	fast adsorption rate wihin 40 minutes	996:1032	The affinity chromatographic microspheres are prepared by immobilizing Cu2+, which exhibits high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb), fast adsorption rate wihin 40 minutes, well-selectivity, and excellent recyclability in ten cycles.
33373794	2	33	theme	crosslinking	348:359	arg1	reaction					361:368	The crosslinking reaction	344:368	The crosslinking reaction	344:368	The crosslinking reaction homogeneously proceeds between polysaccharides, and the covalent bonding network is generated to replace the inherent hydrogen bonding network of cellulose.
33373794	2	34	theme	hydrogen	488:495	arg1	network					505:511	the inherent hydrogen bonding network	475:511	the inherent hydrogen bonding network of cellulose	475:524	The crosslinking reaction homogeneously proceeds between polysaccharides, and the covalent bonding network is generated to replace the inherent hydrogen bonding network of cellulose.
33373794	2	35	theme	bonding	497:503	arg1	network					505:511	the inherent hydrogen bonding network	475:511	the inherent hydrogen bonding network of cellulose	475:524	The crosslinking reaction homogeneously proceeds between polysaccharides, and the covalent bonding network is generated to replace the inherent hydrogen bonding network of cellulose.
33373794	0	36	theme	proteins	159:166	arg1	separation					130:139	enhanced selective separation	111:139	enhanced selective separation of histidine-rich proteins	111:166	Preparation and characterization of highly porous cellulose-agarose composite chromatographic microspheres for enhanced selective separation of histidine-rich proteins.
33373794	3	37	theme	%	587:587	arg1	crystallinity					565:577	low crystallinity	561:577	low crystallinity	561:577	The prepared microspheres exhibit low crystallinity of 12.45%, which means high content of amorphous regions.
33373794	1	38	theme	surface	244:250	arg1	area					252:255	high specific surface area	230:255	high specific surface area	230:255	In this work, the porous cellulose-agarose microspheres with high specific surface area and enhanced mechanical strength are prepared by a novel chemical crosslinking method.
33373794	0	39	theme	porous	43:48	arg1	microspheres					94:105	highly porous cellulose-agarose composite chromatographic microspheres	36:105	highly porous cellulose-agarose composite chromatographic microspheres	36:105	Preparation and characterization of highly porous cellulose-agarose composite chromatographic microspheres for enhanced selective separation of histidine-rich proteins.
33373794	5	40	theme	adsorption	1001:1010	arg1	rate					1012:1015	fast adsorption rate	996:1015	fast adsorption rate wihin 40 minutes	996:1032	The affinity chromatographic microspheres are prepared by immobilizing Cu2+, which exhibits high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb), fast adsorption rate wihin 40 minutes, well-selectivity, and excellent recyclability in ten cycles.
33373794	6	41	theme	outstanding	1135:1145	arg1	candidate					1147:1155	an outstanding candidate	1132:1155	an outstanding candidate for the high performance of biomacromolecular purification	1132:1214	We expect that this work to provide an outstanding candidate for the high performance of biomacromolecular purification.
33373794	5	42	theme	mg/g	962:965	arg1	well-selectivity					1035:1050	well-selectivity	1035:1050	well-selectivity	1035:1050	The affinity chromatographic microspheres are prepared by immobilizing Cu2+, which exhibits high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb), fast adsorption rate wihin 40 minutes, well-selectivity, and excellent recyclability in ten cycles.
33373794	5	42	theme	mg/g	962:965	arg1	rate					1012:1015	fast adsorption rate	996:1015	fast adsorption rate wihin 40 minutes	996:1032	The affinity chromatographic microspheres are prepared by immobilizing Cu2+, which exhibits high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb), fast adsorption rate wihin 40 minutes, well-selectivity, and excellent recyclability in ten cycles.
33373794	5	42	theme	mg/g	962:965	arg1	capacity					943:950	high adsorption capacity	927:950	high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb)	927:993	The affinity chromatographic microspheres are prepared by immobilizing Cu2+, which exhibits high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb), fast adsorption rate wihin 40 minutes, well-selectivity, and excellent recyclability in ten cycles.
33373794	5	42	theme	mg/g	962:965	arg1	recyclability					1067:1079	excellent recyclability	1057:1079	excellent recyclability in ten cycles	1057:1093	The affinity chromatographic microspheres are prepared by immobilizing Cu2+, which exhibits high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb), fast adsorption rate wihin 40 minutes, well-selectivity, and excellent recyclability in ten cycles.
33373794	0	43	theme	composite	68:76	arg1	microspheres					94:105	highly porous cellulose-agarose composite chromatographic microspheres	36:105	highly porous cellulose-agarose composite chromatographic microspheres	36:105	Preparation and characterization of highly porous cellulose-agarose composite chromatographic microspheres for enhanced selective separation of histidine-rich proteins.
33373794	5	44	dep	rate	1012:1015	arg1	minutes					1026:1032	wihin 40 minutes	1017:1032	fast adsorption rate wihin 40 minutes	996:1032	The affinity chromatographic microspheres are prepared by immobilizing Cu2+, which exhibits high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb), fast adsorption rate wihin 40 minutes, well-selectivity, and excellent recyclability in ten cycles.
33373794	5	45	theme	chromatographic	848:862	arg1	microspheres					864:875	The affinity chromatographic microspheres	835:875	The affinity chromatographic microspheres	835:875	The affinity chromatographic microspheres are prepared by immobilizing Cu2+, which exhibits high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb), fast adsorption rate wihin 40 minutes, well-selectivity, and excellent recyclability in ten cycles.
33373794	6	46	theme	biomacromolecular	1185:1201	arg1	purification					1203:1214	biomacromolecular purification	1185:1214	biomacromolecular purification	1185:1214	We expect that this work to provide an outstanding candidate for the high performance of biomacromolecular purification.
33373794	4	47	theme	surface	806:812	arg1	area					814:817	high specific surface area	792:817	high specific surface area of 183.81 m2/g	792:832	The micro-meso-macroporous structure of microspheres in morphology is conducive to high permeability and adsorption capacity, and the microspheres possess high specific surface area of 183.81 m2/g.
33373794	0	48	theme	cellulose-agarose	50:66	arg1	microspheres					94:105	highly porous cellulose-agarose composite chromatographic microspheres	36:105	highly porous cellulose-agarose composite chromatographic microspheres	36:105	Preparation and characterization of highly porous cellulose-agarose composite chromatographic microspheres for enhanced selective separation of histidine-rich proteins.
33373794	4	49	theme	specific	797:804	arg1	area					814:817	high specific surface area	792:817	high specific surface area of 183.81 m2/g	792:832	The micro-meso-macroporous structure of microspheres in morphology is conducive to high permeability and adsorption capacity, and the microspheres possess high specific surface area of 183.81 m2/g.
33373794	5	50	from	capacity	943:950	arg1	cycles					1088:1093	ten cycles	1084:1093	ten cycles	1084:1093	The affinity chromatographic microspheres are prepared by immobilizing Cu2+, which exhibits high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb), fast adsorption rate wihin 40 minutes, well-selectivity, and excellent recyclability in ten cycles.
33373794	3	51	theme	amorphous	618:626	arg1	regions					628:634	amorphous regions	618:634	amorphous regions	618:634	The prepared microspheres exhibit low crystallinity of 12.45%, which means high content of amorphous regions.
33373794	1	52	theme	enhanced	261:268	arg1	strength					281:288	enhanced mechanical strength	261:288	enhanced mechanical strength	261:288	In this work, the porous cellulose-agarose microspheres with high specific surface area and enhanced mechanical strength are prepared by a novel chemical crosslinking method.
33373794	1	53	with	microspheres	212:223	arg1	strength					281:288	enhanced mechanical strength	261:288	enhanced mechanical strength	261:288	In this work, the porous cellulose-agarose microspheres with high specific surface area and enhanced mechanical strength are prepared by a novel chemical crosslinking method.
33373794	1	53	with	microspheres	212:223	arg1	area					252:255	high specific surface area	230:255	high specific surface area	230:255	In this work, the porous cellulose-agarose microspheres with high specific surface area and enhanced mechanical strength are prepared by a novel chemical crosslinking method.
33373794	5	54	theme	high	927:930	arg1	capacity					943:950	high adsorption capacity	927:950	high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb)	927:993	The affinity chromatographic microspheres are prepared by immobilizing Cu2+, which exhibits high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb), fast adsorption rate wihin 40 minutes, well-selectivity, and excellent recyclability in ten cycles.
33373794	3	55	theme	low	561:563	arg1	crystallinity					565:577	low crystallinity	561:577	low crystallinity	561:577	The prepared microspheres exhibit low crystallinity of 12.45%, which means high content of amorphous regions.
33373794	1	56	theme	mechanical	270:279	arg1	strength					281:288	enhanced mechanical strength	261:288	enhanced mechanical strength	261:288	In this work, the porous cellulose-agarose microspheres with high specific surface area and enhanced mechanical strength are prepared by a novel chemical crosslinking method.
33373794	0	57	theme	microspheres	94:105	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of highly porous cellulose-agarose composite chromatographic microspheres for enhanced selective separation of histidine-rich proteins.
33373794	0	57	theme	microspheres	94:105	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of highly porous cellulose-agarose composite chromatographic microspheres for enhanced selective separation of histidine-rich proteins.
33373794	1	58	theme	specific	235:242	arg1	area					252:255	high specific surface area	230:255	high specific surface area	230:255	In this work, the porous cellulose-agarose microspheres with high specific surface area and enhanced mechanical strength are prepared by a novel chemical crosslinking method.
33373794	2	59	theme	bonding	435:441	arg1	network					443:449	the covalent bonding network	422:449	the covalent bonding network	422:449	The crosslinking reaction homogeneously proceeds between polysaccharides, and the covalent bonding network is generated to replace the inherent hydrogen bonding network of cellulose.
33373794	5	60	from	well-selectivity	1035:1050	arg1	cycles					1088:1093	ten cycles	1084:1093	ten cycles	1084:1093	The affinity chromatographic microspheres are prepared by immobilizing Cu2+, which exhibits high adsorption capacity of 197.65 mg/g for bovine hemoglobin (BHb), fast adsorption rate wihin 40 minutes, well-selectivity, and excellent recyclability in ten cycles.
33373794	0	61	theme	chromatographic	78:92	arg1	microspheres					94:105	highly porous cellulose-agarose composite chromatographic microspheres	36:105	highly porous cellulose-agarose composite chromatographic microspheres	36:105	Preparation and characterization of highly porous cellulose-agarose composite chromatographic microspheres for enhanced selective separation of histidine-rich proteins.
33373794	4	62	contain	possess	784:790	arg2	area					814:817	high specific surface area	792:817	high specific surface area of 183.81 m2/g	792:832	The micro-meso-macroporous structure of microspheres in morphology is conducive to high permeability and adsorption capacity, and the microspheres possess high specific surface area of 183.81 m2/g.
33373794	4	62	contain	possess	784:790	arg1	microspheres					771:782	the microspheres	767:782	the microspheres	767:782	The micro-meso-macroporous structure of microspheres in morphology is conducive to high permeability and adsorption capacity, and the microspheres possess high specific surface area of 183.81 m2/g.
33373794	4	63	theme	high	720:723	arg1	permeability					725:736	high permeability	720:736	high permeability	720:736	The micro-meso-macroporous structure of microspheres in morphology is conducive to high permeability and adsorption capacity, and the microspheres possess high specific surface area of 183.81 m2/g.
33373794	2	64	theme	covalent	426:433	arg1	network					443:449	the covalent bonding network	422:449	the covalent bonding network	422:449	The crosslinking reaction homogeneously proceeds between polysaccharides, and the covalent bonding network is generated to replace the inherent hydrogen bonding network of cellulose.
32070735	5	0	theme	droplets	745:752	arg1	surface					730:736	the surface	726:736	the surface of oil droplets and the gel-like network structure in the continuous-phase	726:811	CLSM and cryo-SEM showed the anchoring of ZHPs onto the surface of oil droplets and the gel-like network structure in the continuous-phase.
32070735	4	1	theme	ZHP	514:516	arg1	composite					518:526	The ZHP composite	510:526	The ZHP composite at the HP-ZP ratio of 1:1 (w/w)	510:558	The ZHP composite at the HP-ZP ratio of 1:1 (w/w) exhibited near-neutral wettability (92.9o ± 1.01), thereby being used for stabilizing Pickering emulsion (ZHPEs).
32070735	9	2	theme	particle	1179:1186	arg1	shell-materials					1188:1202	particle shell-materials	1179:1202	particle shell-materials	1179:1202	Therefore, antioxidative polysaccharides could stabilize Pickering emulsions as particle shell-materials while offering protection on lipid components against oxidation.
32070735	2	3	from	pomace	332:337	arg1	pectin					306:311	antioxidative pectin	292:311	antioxidative pectin from hawthorn wine pomace (HP)	292:342	In this study, antioxidative pectin from hawthorn wine pomace (HP) was first produced.
32070735	5	4	from	structure	779:787	arg1	continuous-phase					796:811	the continuous-phase	792:811	the continuous-phase	792:811	CLSM and cryo-SEM showed the anchoring of ZHPs onto the surface of oil droplets and the gel-like network structure in the continuous-phase.
32070735	8	5	theme	antioxidant	1005:1015	arg1	capacity					1017:1024	The antioxidant capacity	1001:1024	The antioxidant capacity of HP	1001:1030	The antioxidant capacity of HP helped protect the Pickering emulsion against its lipid oxidation.
32070735	1	6	theme	biodegradable	172:184	arg1	nanoparticles					194:206	natural and biodegradable polymer nanoparticles	160:206	natural and biodegradable polymer nanoparticles as Pickering emulsion stabilizers	160:240	The development of natural and biodegradable polymer nanoparticles as Pickering emulsion stabilizers has attracted increasing interest.
32070735	4	7	theme	near-neutral	570:581	arg1	92.9o ± 1.01					596:607	92.9o ± 1.01	596:607	92.9o ± 1.01	596:607	The ZHP composite at the HP-ZP ratio of 1:1 (w/w) exhibited near-neutral wettability (92.9o ± 1.01), thereby being used for stabilizing Pickering emulsion (ZHPEs).
32070735	4	7	theme	near-neutral	570:581	arg1	wettability					583:593	near-neutral wettability	570:593	near-neutral wettability (92.9o ± 1.01)	570:608	The ZHP composite at the HP-ZP ratio of 1:1 (w/w) exhibited near-neutral wettability (92.9o ± 1.01), thereby being used for stabilizing Pickering emulsion (ZHPEs).
32070735	0	8	theme	zein-pectin	103:113	arg1	structure					130:138	zein-pectin gel-like shell structure	103:138	zein-pectin gel-like shell structure	103:138	Antioxidative pectin from hawthorn wine pomace stabilizes and protects Pickering emulsions via forming zein-pectin gel-like shell structure.
32070735	6	9	theme	elastic-solid	882:894	arg1	characteristics					896:910	elastic-solid characteristics	882:910	elastic-solid characteristics	882:910	ZHPEs at 0.5-0.7 (v/v) oil fractions were pseudoplastic fluids with elastic-solid characteristics.
32070735	8	10	theme	HP	1029:1030	arg1	capacity					1017:1024	The antioxidant capacity	1001:1024	The antioxidant capacity of HP	1001:1030	The antioxidant capacity of HP helped protect the Pickering emulsion against its lipid oxidation.
32070735	6	11	theme	oil	837:839	arg1	fractions					841:849	0.5-0.7 (v/v) oil fractions	823:849	0.5-0.7 (v/v) oil fractions	823:849	ZHPEs at 0.5-0.7 (v/v) oil fractions were pseudoplastic fluids with elastic-solid characteristics.
32070735	2	12	theme	wine	327:330	arg1	HP					340:341	HP	340:341	HP	340:341	In this study, antioxidative pectin from hawthorn wine pomace (HP) was first produced.
32070735	2	12	theme	wine	327:330	arg1	pomace					332:337	hawthorn wine pomace	318:337	hawthorn wine pomace (HP)	318:342	In this study, antioxidative pectin from hawthorn wine pomace (HP) was first produced.
32070735	9	13	theme	Pickering	1156:1164	arg1	emulsions					1166:1174	Pickering emulsions	1156:1174	Pickering emulsions	1156:1174	Therefore, antioxidative polysaccharides could stabilize Pickering emulsions as particle shell-materials while offering protection on lipid components against oxidation.
32070735	5	14	from	continuous-phase	796:811	arg1	surface					730:736	the surface	726:736	the surface of oil droplets and the gel-like network structure in the continuous-phase	726:811	CLSM and cryo-SEM showed the anchoring of ZHPs onto the surface of oil droplets and the gel-like network structure in the continuous-phase.
32070735	2	15	theme	hawthorn	318:325	arg1	HP					340:341	HP	340:341	HP	340:341	In this study, antioxidative pectin from hawthorn wine pomace (HP) was first produced.
32070735	2	15	theme	hawthorn	318:325	arg1	pomace					332:337	hawthorn wine pomace	318:337	hawthorn wine pomace (HP)	318:342	In this study, antioxidative pectin from hawthorn wine pomace (HP) was first produced.
32070735	10	16	theme	waste	1333:1337	arg1	utilization					1313:1323	the sustainable utilization	1297:1323	the sustainable utilization of food waste for producing value-added products	1297:1372	This study has demonstrated the sustainable utilization of food waste for producing value-added products.
32070735	0	17	theme	shell	124:128	arg1	structure					130:138	zein-pectin gel-like shell structure	103:138	zein-pectin gel-like shell structure	103:138	Antioxidative pectin from hawthorn wine pomace stabilizes and protects Pickering emulsions via forming zein-pectin gel-like shell structure.
32070735	7	18	dep	0.6	924:926	arg1	v/v					937:939	v/v	937:939	v/v	937:939	ZHPEs with 0.6 and 0.7 (v/v) oil fractions showed excellent thermal stability 20-60 °C.
32070735	10	19	theme	food	1328:1331	arg1	waste					1333:1337	food waste	1328:1337	food waste	1328:1337	This study has demonstrated the sustainable utilization of food waste for producing value-added products.
32070735	1	20	theme	polymer	186:192	arg1	nanoparticles					194:206	natural and biodegradable polymer nanoparticles	160:206	natural and biodegradable polymer nanoparticles as Pickering emulsion stabilizers	160:240	The development of natural and biodegradable polymer nanoparticles as Pickering emulsion stabilizers has attracted increasing interest.
32070735	0	21	theme	gel-like	115:122	arg1	structure					130:138	zein-pectin gel-like shell structure	103:138	zein-pectin gel-like shell structure	103:138	Antioxidative pectin from hawthorn wine pomace stabilizes and protects Pickering emulsions via forming zein-pectin gel-like shell structure.
32070735	0	22	from	pomace	40:45	arg1	pectin					14:19	Antioxidative pectin	0:19	Antioxidative pectin from hawthorn wine pomace	0:45	Antioxidative pectin from hawthorn wine pomace stabilizes and protects Pickering emulsions via forming zein-pectin gel-like shell structure.
32070735	1	23	theme	nanoparticles	194:206	arg1	development					145:155	The development	141:155	The development of natural and biodegradable polymer nanoparticles as Pickering emulsion stabilizers	141:240	The development of natural and biodegradable polymer nanoparticles as Pickering emulsion stabilizers has attracted increasing interest.
32070735	7	24	with	ZHPEs	913:917	arg1	fractions					946:954	0.6 and 0.7 (v/v) oil fractions	924:954	0.6 and 0.7 (v/v) oil fractions	924:954	ZHPEs with 0.6 and 0.7 (v/v) oil fractions showed excellent thermal stability 20-60 °C.
32070735	8	25	theme	Pickering	1051:1059	arg1	emulsion					1061:1068	the Pickering emulsion	1047:1068	the Pickering emulsion	1047:1068	The antioxidant capacity of HP helped protect the Pickering emulsion against its lipid oxidation.
32070735	9	26	theme	lipid	1233:1237	arg1	components					1239:1248	lipid components	1233:1248	lipid components	1233:1248	Therefore, antioxidative polysaccharides could stabilize Pickering emulsions as particle shell-materials while offering protection on lipid components against oxidation.
32070735	0	27	theme	Antioxidative	0:12	arg1	pectin					14:19	Antioxidative pectin	0:19	Antioxidative pectin from hawthorn wine pomace	0:45	Antioxidative pectin from hawthorn wine pomace stabilizes and protects Pickering emulsions via forming zein-pectin gel-like shell structure.
32070735	5	28	theme	ZHPs	716:719	arg1	anchoring					703:711	the anchoring	699:711	the anchoring of ZHPs onto the surface of oil droplets and the gel-like network structure in the continuous-phase	699:811	CLSM and cryo-SEM showed the anchoring of ZHPs onto the surface of oil droplets and the gel-like network structure in the continuous-phase.
32070735	1	29	theme	Pickering	211:219	arg1	stabilizers					230:240	Pickering emulsion stabilizers	211:240	Pickering emulsion stabilizers	211:240	The development of natural and biodegradable polymer nanoparticles as Pickering emulsion stabilizers has attracted increasing interest.
32070735	0	30	theme	hawthorn	26:33	arg1	pomace					40:45	hawthorn wine pomace	26:45	hawthorn wine pomace	26:45	Antioxidative pectin from hawthorn wine pomace stabilizes and protects Pickering emulsions via forming zein-pectin gel-like shell structure.
32070735	5	31	theme	network	771:777	arg1	structure					779:787	the gel-like network structure	758:787	the gel-like network structure in the continuous-phase	758:811	CLSM and cryo-SEM showed the anchoring of ZHPs onto the surface of oil droplets and the gel-like network structure in the continuous-phase.
32070735	10	32	theme	value-added	1353:1363	arg1	products					1365:1372	value-added products	1353:1372	value-added products	1353:1372	This study has demonstrated the sustainable utilization of food waste for producing value-added products.
32070735	1	33	theme	emulsion	221:228	arg1	stabilizers					230:240	Pickering emulsion stabilizers	211:240	Pickering emulsion stabilizers	211:240	The development of natural and biodegradable polymer nanoparticles as Pickering emulsion stabilizers has attracted increasing interest.
32070735	6	34	with	fluids	870:875	arg1	characteristics					896:910	elastic-solid characteristics	882:910	elastic-solid characteristics	882:910	ZHPEs at 0.5-0.7 (v/v) oil fractions were pseudoplastic fluids with elastic-solid characteristics.
32070735	3	35	theme	zein-HP	419:425	arg1	ZHPs					452:455	ZHPs	452:455	ZHPs	452:455	HP and zein-nanoparticles (ZPs) were used to fabricate zein-HP composite nanoparticles (ZHPs) via hydrogen bonding and electrostatic interaction.
32070735	3	35	theme	zein-HP	419:425	arg1	nanoparticles					437:449	zein-HP composite nanoparticles	419:449	zein-HP composite nanoparticles (ZHPs)	419:456	HP and zein-nanoparticles (ZPs) were used to fabricate zein-HP composite nanoparticles (ZHPs) via hydrogen bonding and electrostatic interaction.
32070735	3	36	used	used	401:404	arg2	ZPs					391:393	ZPs	391:393	ZPs	391:393	HP and zein-nanoparticles (ZPs) were used to fabricate zein-HP composite nanoparticles (ZHPs) via hydrogen bonding and electrostatic interaction.
32070735	3	36	used	used	401:404	arg2	zein-nanoparticles					371:388	zein-nanoparticles	371:388	zein-nanoparticles (ZPs)	371:394	HP and zein-nanoparticles (ZPs) were used to fabricate zein-HP composite nanoparticles (ZHPs) via hydrogen bonding and electrostatic interaction.
32070735	3	36	used	used	401:404	arg2	HP					364:365	HP	364:365	HP	364:365	HP and zein-nanoparticles (ZPs) were used to fabricate zein-HP composite nanoparticles (ZHPs) via hydrogen bonding and electrostatic interaction.
32070735	5	37	from	surface	730:736	arg1	continuous-phase					796:811	the continuous-phase	792:811	the continuous-phase	792:811	CLSM and cryo-SEM showed the anchoring of ZHPs onto the surface of oil droplets and the gel-like network structure in the continuous-phase.
32070735	4	38	theme	Pickering	646:654	arg1	ZHPEs					666:670	Pickering emulsion (ZHPEs)	646:671	Pickering emulsion (ZHPEs)	646:671	The ZHP composite at the HP-ZP ratio of 1:1 (w/w) exhibited near-neutral wettability (92.9o ± 1.01), thereby being used for stabilizing Pickering emulsion (ZHPEs).
32070735	0	39	theme	wine	35:38	arg1	pomace					40:45	hawthorn wine pomace	26:45	hawthorn wine pomace	26:45	Antioxidative pectin from hawthorn wine pomace stabilizes and protects Pickering emulsions via forming zein-pectin gel-like shell structure.
32070735	3	40	theme	composite	427:435	arg1	ZHPs					452:455	ZHPs	452:455	ZHPs	452:455	HP and zein-nanoparticles (ZPs) were used to fabricate zein-HP composite nanoparticles (ZHPs) via hydrogen bonding and electrostatic interaction.
32070735	3	40	theme	composite	427:435	arg1	nanoparticles					437:449	zein-HP composite nanoparticles	419:449	zein-HP composite nanoparticles (ZHPs)	419:456	HP and zein-nanoparticles (ZPs) were used to fabricate zein-HP composite nanoparticles (ZHPs) via hydrogen bonding and electrostatic interaction.
32070735	6	41	from	fractions	841:849	arg1	ZHPEs					814:818	ZHPEs	814:818	ZHPEs at 0.5-0.7 (v/v) oil fractions	814:849	ZHPEs at 0.5-0.7 (v/v) oil fractions were pseudoplastic fluids with elastic-solid characteristics.
32070735	9	42	theme	antioxidative	1110:1122	arg1	polysaccharides					1124:1138	antioxidative polysaccharides	1110:1138	antioxidative polysaccharides	1110:1138	Therefore, antioxidative polysaccharides could stabilize Pickering emulsions as particle shell-materials while offering protection on lipid components against oxidation.
32070735	3	43	theme	hydrogen	462:469	arg1	bonding					471:477	hydrogen bonding	462:477	hydrogen bonding	462:477	HP and zein-nanoparticles (ZPs) were used to fabricate zein-HP composite nanoparticles (ZHPs) via hydrogen bonding and electrostatic interaction.
32070735	4	44	from	ratio	541:545	arg1	composite					518:526	The ZHP composite	510:526	The ZHP composite at the HP-ZP ratio of 1:1 (w/w)	510:558	The ZHP composite at the HP-ZP ratio of 1:1 (w/w) exhibited near-neutral wettability (92.9o ± 1.01), thereby being used for stabilizing Pickering emulsion (ZHPEs).
32070735	4	45	theme	HP-ZP	535:539	arg1	ratio					541:545	the HP-ZP ratio	531:545	the HP-ZP ratio of 1:1 (w/w)	531:558	The ZHP composite at the HP-ZP ratio of 1:1 (w/w) exhibited near-neutral wettability (92.9o ± 1.01), thereby being used for stabilizing Pickering emulsion (ZHPEs).
32070735	6	46	theme	pseudoplastic	856:868	arg1	fluids					870:875	pseudoplastic fluids	856:875	pseudoplastic fluids with elastic-solid characteristics	856:910	ZHPEs at 0.5-0.7 (v/v) oil fractions were pseudoplastic fluids with elastic-solid characteristics.
32070735	5	47	theme	structure	779:787	arg1	surface					730:736	the surface	726:736	the surface of oil droplets and the gel-like network structure in the continuous-phase	726:811	CLSM and cryo-SEM showed the anchoring of ZHPs onto the surface of oil droplets and the gel-like network structure in the continuous-phase.
32070735	4	48	theme	1:1	550:552	arg1	ratio					541:545	the HP-ZP ratio	531:545	the HP-ZP ratio of 1:1 (w/w)	531:558	The ZHP composite at the HP-ZP ratio of 1:1 (w/w) exhibited near-neutral wettability (92.9o ± 1.01), thereby being used for stabilizing Pickering emulsion (ZHPEs).
32070735	5	49	from	droplets	745:752	arg1	continuous-phase					796:811	the continuous-phase	792:811	the continuous-phase	792:811	CLSM and cryo-SEM showed the anchoring of ZHPs onto the surface of oil droplets and the gel-like network structure in the continuous-phase.
32070735	8	50	theme	lipid	1082:1086	arg1	oxidation					1088:1096	its lipid oxidation	1078:1096	its lipid oxidation	1078:1096	The antioxidant capacity of HP helped protect the Pickering emulsion against its lipid oxidation.
32070735	6	51	theme	0.5-0.7	823:829	arg1	fractions					841:849	0.5-0.7 (v/v) oil fractions	823:849	0.5-0.7 (v/v) oil fractions	823:849	ZHPEs at 0.5-0.7 (v/v) oil fractions were pseudoplastic fluids with elastic-solid characteristics.
32070735	2	52	theme	antioxidative	292:304	arg1	pectin					306:311	antioxidative pectin	292:311	antioxidative pectin from hawthorn wine pomace (HP)	292:342	In this study, antioxidative pectin from hawthorn wine pomace (HP) was first produced.
32070735	7	53	theme	oil	942:944	arg1	fractions					946:954	0.6 and 0.7 (v/v) oil fractions	924:954	0.6 and 0.7 (v/v) oil fractions	924:954	ZHPEs with 0.6 and 0.7 (v/v) oil fractions showed excellent thermal stability 20-60 °C.
32070735	3	54	theme	electrostatic	483:495	arg1	interaction					497:507	electrostatic interaction	483:507	electrostatic interaction	483:507	HP and zein-nanoparticles (ZPs) were used to fabricate zein-HP composite nanoparticles (ZHPs) via hydrogen bonding and electrostatic interaction.
32070735	1	55	theme	increasing	256:265	arg1	interest					267:274	increasing interest	256:274	increasing interest	256:274	The development of natural and biodegradable polymer nanoparticles as Pickering emulsion stabilizers has attracted increasing interest.
32070735	7	56	theme	excellent	963:971	arg1	stability					981:989	excellent thermal stability 20-60 °C	963:998	excellent thermal stability 20-60 °C	963:998	ZHPEs with 0.6 and 0.7 (v/v) oil fractions showed excellent thermal stability 20-60 °C.
32070735	9	57	from	protection	1219:1228	arg1	components					1239:1248	lipid components	1233:1248	lipid components	1233:1248	Therefore, antioxidative polysaccharides could stabilize Pickering emulsions as particle shell-materials while offering protection on lipid components against oxidation.
32070735	0	58	theme	Pickering	71:79	arg1	emulsions					81:89	Pickering emulsions	71:89	Pickering emulsions	71:89	Antioxidative pectin from hawthorn wine pomace stabilizes and protects Pickering emulsions via forming zein-pectin gel-like shell structure.
32070735	7	59	theme	thermal	973:979	arg1	stability					981:989	excellent thermal stability 20-60 °C	963:998	excellent thermal stability 20-60 °C	963:998	ZHPEs with 0.6 and 0.7 (v/v) oil fractions showed excellent thermal stability 20-60 °C.
32070735	4	60	theme	emulsion	656:663	arg1	ZHPEs					666:670	Pickering emulsion (ZHPEs)	646:671	Pickering emulsion (ZHPEs)	646:671	The ZHP composite at the HP-ZP ratio of 1:1 (w/w) exhibited near-neutral wettability (92.9o ± 1.01), thereby being used for stabilizing Pickering emulsion (ZHPEs).
32070735	5	61	theme	oil	741:743	arg1	droplets					745:752	oil droplets	741:752	oil droplets	741:752	CLSM and cryo-SEM showed the anchoring of ZHPs onto the surface of oil droplets and the gel-like network structure in the continuous-phase.
32070735	5	62	theme	gel-like	762:769	arg1	structure					779:787	the gel-like network structure	758:787	the gel-like network structure in the continuous-phase	758:811	CLSM and cryo-SEM showed the anchoring of ZHPs onto the surface of oil droplets and the gel-like network structure in the continuous-phase.
32070735	1	63	theme	natural	160:166	arg1	nanoparticles					194:206	natural and biodegradable polymer nanoparticles	160:206	natural and biodegradable polymer nanoparticles as Pickering emulsion stabilizers	160:240	The development of natural and biodegradable polymer nanoparticles as Pickering emulsion stabilizers has attracted increasing interest.
32070735	10	64	theme	sustainable	1301:1311	arg1	utilization					1313:1323	the sustainable utilization	1297:1323	the sustainable utilization of food waste for producing value-added products	1297:1372	This study has demonstrated the sustainable utilization of food waste for producing value-added products.
33670269	3	0	theme	green	311:315	arg1	Cellulose					291:299	Bacterial Cellulose	281:299	Bacterial Cellulose (BC)	281:304	Bacterial Cellulose (BC) is a green biopolymer, with significant mechanical and electrical properties.
33670269	3	0	theme	green	311:315	arg1	biopolymer					317:326	a green biopolymer	309:326	a green biopolymer	309:326	Bacterial Cellulose (BC) is a green biopolymer, with significant mechanical and electrical properties.
33670269	9	1	theme	transduction	982:993	arg1	performance					995:1005	the transduction performance	978:1005	the transduction performance	978:1005	The influence of ILs in the transduction performance, both in the case of OC voltage and SC current is investigated.
33670269	10	2	theme	properties	1159:1168	arg1	investigations					1084:1097	Experimental investigations	1071:1097	Experimental investigations of structural, chemical, and mechanoelectrical transduction properties, when the composite is dynamically bent,	1071:1209	Experimental investigations of structural, chemical, and mechanoelectrical transduction properties, when the composite is dynamically bent, are performed.
33670269	11	3	theme	frequency	1310:1318	arg1	domains					1320:1326	the frequency domains	1306:1326	the frequency domains	1306:1326	The mechanoelectrical investigation has been carried on both in the time and in the frequency domains.
33670269	13	4	theme	magnitude	1572:1580	arg1	orders					1562:1567	about two orders	1552:1567	about two orders of magnitude	1552:1580	On the contrary, dramatic changes are observed for the case of SC current, whose value increases by about two orders of magnitude.
33670269	5	5	theme	Conducting	587:596	arg1	Polymer					598:604	Conducting Polymer	587:604	Conducting Polymer (CP)	587:609	The transductors consist of a sheet of BC, impregnated of Ionic Liquids (ILs), and covered with two layers of Conducting Polymer (CP) as the electrodes.
33670269	5	5	theme	Conducting	587:596	arg1	CP					607:608	CP	607:608	CP	607:608	The transductors consist of a sheet of BC, impregnated of Ionic Liquids (ILs), and covered with two layers of Conducting Polymer (CP) as the electrodes.
33670269	10	6	theme	transduction	1146:1157	arg1	properties					1159:1168	structural, chemical, and mechanoelectrical transduction properties	1102:1168	structural, chemical, and mechanoelectrical transduction properties	1102:1168	Experimental investigations of structural, chemical, and mechanoelectrical transduction properties, when the composite is dynamically bent, are performed.
33670269	0	7	theme	Bacterial	76:84	arg1	Transducers					120:130	Bacterial Cellulose-Based Mechanoelectrical Transducers	76:130	Bacterial Cellulose-Based Mechanoelectrical Transducers	76:130	Investigation on the Role of Ionic Liquids in the Output Signal Produced by Bacterial Cellulose-Based Mechanoelectrical Transducers.
33670269	0	8	from	Investigation	0:12	arg1	Role					21:24	the Role	17:24	the Role of Ionic Liquids in the Output Signal	17:62	Investigation on the Role of Ionic Liquids in the Output Signal Produced by Bacterial Cellulose-Based Mechanoelectrical Transducers.
33670269	5	9	theme	Polymer	598:604	arg1	layers					577:582	two layers	573:582	two layers of Conducting Polymer (CP)	573:609	The transductors consist of a sheet of BC, impregnated of Ionic Liquids (ILs), and covered with two layers of Conducting Polymer (CP) as the electrodes.
33670269	7	10	theme	Generating	697:706	arg1	sensors					708:714	Generating sensors	697:714	Generating sensors	697:714	Generating sensors can produce either Open Circuit (OC) voltage or Short Circuit (SC) current.
33670269	3	11	theme	electrical	361:370	arg1	properties					372:381	significant mechanical and electrical properties	334:381	significant mechanical and electrical properties	334:381	Bacterial Cellulose (BC) is a green biopolymer, with significant mechanical and electrical properties.
33670269	12	12	theme	relevant	1359:1366	arg1	changes					1368:1374	no relevant changes	1356:1374	no relevant changes	1356:1374	Reported results show that no relevant changes can be obtained because of the use of IL when the OC voltage is considered.
33670269	9	13	theme	current	1046:1052	arg1	case					1020:1023	the case	1016:1023	the case of OC voltage and SC current	1016:1052	The influence of ILs in the transduction performance, both in the case of OC voltage and SC current is investigated.
33670269	12	14	theme	Reported	1329:1336	arg1	results					1338:1344	Reported results	1329:1344	Reported results	1329:1344	Reported results show that no relevant changes can be obtained because of the use of IL when the OC voltage is considered.
33670269	5	15	theme	BC	516:517	arg1	sheet					507:511	a sheet	505:511	a sheet of BC	505:517	The transductors consist of a sheet of BC, impregnated of Ionic Liquids (ILs), and covered with two layers of Conducting Polymer (CP) as the electrodes.
33670269	3	16	theme	significant	334:344	arg1	properties					372:381	significant mechanical and electrical properties	334:381	significant mechanical and electrical properties	334:381	Bacterial Cellulose (BC) is a green biopolymer, with significant mechanical and electrical properties.
33670269	8	17	theme	dynamic	920:926	arg1	deformation					928:938	dynamic deformation	920:938	dynamic deformation	920:938	In the paper, the OC voltage and SC current, generated from BC-based composites, in a cantilever configuration and subjected to dynamic deformation are compared.
33670269	8	18	theme	OC	810:811	arg1	voltage					813:819	the OC voltage	806:819	the OC voltage	806:819	In the paper, the OC voltage and SC current, generated from BC-based composites, in a cantilever configuration and subjected to dynamic deformation are compared.
33670269	0	19	theme	Cellulose-Based	86:100	arg1	Transducers					120:130	Bacterial Cellulose-Based Mechanoelectrical Transducers	76:130	Bacterial Cellulose-Based Mechanoelectrical Transducers	76:130	Investigation on the Role of Ionic Liquids in the Output Signal Produced by Bacterial Cellulose-Based Mechanoelectrical Transducers.
33670269	9	20	dep	performance	995:1005	arg1	both					1008:1011	both	1008:1011	both	1008:1011	The influence of ILs in the transduction performance, both in the case of OC voltage and SC current is investigated.
33670269	13	21	theme	SC	1515:1516	arg1	current					1518:1524	SC current	1515:1524	SC current	1515:1524	On the contrary, dramatic changes are observed for the case of SC current, whose value increases by about two orders of magnitude.
33670269	8	22	theme	SC	825:826	arg1	current					828:834	SC current	825:834	SC current	825:834	In the paper, the OC voltage and SC current, generated from BC-based composites, in a cantilever configuration and subjected to dynamic deformation are compared.
33670269	0	23	theme	Mechanoelectrical	102:118	arg1	Transducers					120:130	Bacterial Cellulose-Based Mechanoelectrical Transducers	76:130	Bacterial Cellulose-Based Mechanoelectrical Transducers	76:130	Investigation on the Role of Ionic Liquids in the Output Signal Produced by Bacterial Cellulose-Based Mechanoelectrical Transducers.
33670269	8	24	dep	current	828:834	arg1	subjected					907:915	subjected	907:915	subjected to dynamic deformation	907:938	In the paper, the OC voltage and SC current, generated from BC-based composites, in a cantilever configuration and subjected to dynamic deformation are compared.
33670269	8	24	dep	current	828:834	arg1	generated					837:845	generated	837:845	generated from BC-based composites, in a cantilever configuration	837:901	In the paper, the OC voltage and SC current, generated from BC-based composites, in a cantilever configuration and subjected to dynamic deformation are compared.
33670269	11	25	theme	mechanoelectrical	1230:1246	arg1	investigation					1248:1260	The mechanoelectrical investigation	1226:1260	The mechanoelectrical investigation	1226:1260	The mechanoelectrical investigation has been carried on both in the time and in the frequency domains.
33670269	8	26	from	composites	861:870	arg1	configuration					889:901	a cantilever configuration	876:901	a cantilever configuration	876:901	In the paper, the OC voltage and SC current, generated from BC-based composites, in a cantilever configuration and subjected to dynamic deformation are compared.
33670269	8	27	theme	cantilever	878:887	arg1	configuration					889:901	a cantilever configuration	876:901	a cantilever configuration	876:901	In the paper, the OC voltage and SC current, generated from BC-based composites, in a cantilever configuration and subjected to dynamic deformation are compared.
33670269	1	28	theme	sustainable	185:195	arg1	economy					197:203	a sustainable economy	183:203	a sustainable economy	183:203	Green sensors are required for the realization of a sustainable economy.
33670269	7	29	theme	SC	779:780	arg1	current					783:789	Short Circuit (SC) current	764:789	Short Circuit (SC) current	764:789	Generating sensors can produce either Open Circuit (OC) voltage or Short Circuit (SC) current.
33670269	4	30	theme	BC-based	384:391	arg1	composites					393:402	BC-based composites	384:402	BC-based composites	384:402	BC-based composites have been proposed to realize generating mechanoelectrical transductors.
33670269	9	31	from	influence	958:966	arg1	performance					995:1005	the transduction performance	978:1005	the transduction performance	978:1005	The influence of ILs in the transduction performance, both in the case of OC voltage and SC current is investigated.
33670269	9	32	theme	OC	1028:1029	arg1	voltage					1031:1037	OC voltage	1028:1037	OC voltage	1028:1037	The influence of ILs in the transduction performance, both in the case of OC voltage and SC current is investigated.
33670269	1	33	theme	economy	197:203	arg1	realization					168:178	the realization	164:178	the realization of a sustainable economy	164:203	Green sensors are required for the realization of a sustainable economy.
33670269	7	34	theme	Short	764:768	arg1	current					783:789	Short Circuit (SC) current	764:789	Short Circuit (SC) current	764:789	Generating sensors can produce either Open Circuit (OC) voltage or Short Circuit (SC) current.
33670269	9	35	theme	voltage	1031:1037	arg1	case					1020:1023	the case	1016:1023	the case of OC voltage and SC current	1016:1052	The influence of ILs in the transduction performance, both in the case of OC voltage and SC current is investigated.
33670269	0	36	theme	Ionic	29:33	arg1	Liquids					35:41	Ionic Liquids	29:41	Ionic Liquids	29:41	Investigation on the Role of Ionic Liquids in the Output Signal Produced by Bacterial Cellulose-Based Mechanoelectrical Transducers.
33670269	7	37	theme	Circuit	770:776	arg1	current					783:789	Short Circuit (SC) current	764:789	Short Circuit (SC) current	764:789	Generating sensors can produce either Open Circuit (OC) voltage or Short Circuit (SC) current.
33670269	13	38	located	observed	1490:1497	arg1	contrary					1459:1466	the contrary	1455:1466	the contrary	1455:1466	On the contrary, dramatic changes are observed for the case of SC current, whose value increases by about two orders of magnitude.
33670269	13	38	located	observed	1490:1497	arg2	changes					1478:1484	dramatic changes	1469:1484	dramatic changes	1469:1484	On the contrary, dramatic changes are observed for the case of SC current, whose value increases by about two orders of magnitude.
33670269	3	39	theme	Bacterial	281:289	arg1	Cellulose					291:299	Bacterial Cellulose	281:299	Bacterial Cellulose (BC)	281:304	Bacterial Cellulose (BC) is a green biopolymer, with significant mechanical and electrical properties.
33670269	3	39	theme	Bacterial	281:289	arg1	biopolymer					317:326	a green biopolymer	309:326	a green biopolymer	309:326	Bacterial Cellulose (BC) is a green biopolymer, with significant mechanical and electrical properties.
33670269	3	39	theme	Bacterial	281:289	arg1	BC					302:303	BC	302:303	BC	302:303	Bacterial Cellulose (BC) is a green biopolymer, with significant mechanical and electrical properties.
33670269	2	40	theme	meaningful	242:251	arg1	solution					253:260	a meaningful solution	240:260	a meaningful solution to such a needing	240:278	Biopolymer-derived composites are a meaningful solution to such a needing.
33670269	2	40	theme	meaningful	242:251	arg1	composites					225:234	Biopolymer-derived composites	206:234	Biopolymer-derived composites	206:234	Biopolymer-derived composites are a meaningful solution to such a needing.
33670269	9	41	theme	SC	1043:1044	arg1	current					1046:1052	SC current	1043:1052	SC current	1043:1052	The influence of ILs in the transduction performance, both in the case of OC voltage and SC current is investigated.
33670269	2	42	link	Biopolymer-derived	206:223	arg1	solution					253:260	a meaningful solution	240:260	a meaningful solution to such a needing	240:278	Biopolymer-derived composites are a meaningful solution to such a needing.
33670269	2	42	link	Biopolymer-derived	206:223	arg1	composites					225:234	Biopolymer-derived composites	206:234	Biopolymer-derived composites	206:234	Biopolymer-derived composites are a meaningful solution to such a needing.
33670269	10	43	theme	Experimental	1071:1082	arg1	investigations					1084:1097	Experimental investigations	1071:1097	Experimental investigations of structural, chemical, and mechanoelectrical transduction properties, when the composite is dynamically bent,	1071:1209	Experimental investigations of structural, chemical, and mechanoelectrical transduction properties, when the composite is dynamically bent, are performed.
33670269	13	44	theme	current	1518:1524	arg1	case					1507:1510	the case	1503:1510	the case	1503:1510	On the contrary, dramatic changes are observed for the case of SC current, whose value increases by about two orders of magnitude.
33670269	12	45	theme	OC	1426:1427	arg1	voltage					1429:1435	the OC voltage	1422:1435	the OC voltage	1422:1435	Reported results show that no relevant changes can be obtained because of the use of IL when the OC voltage is considered.
33670269	8	46	theme	BC-based	852:859	arg1	composites					861:870	BC-based composites	852:870	BC-based composites	852:870	In the paper, the OC voltage and SC current, generated from BC-based composites, in a cantilever configuration and subjected to dynamic deformation are compared.
33670269	0	47	theme	Liquids	35:41	arg1	Role					21:24	the Role	17:24	the Role of Ionic Liquids in the Output Signal	17:62	Investigation on the Role of Ionic Liquids in the Output Signal Produced by Bacterial Cellulose-Based Mechanoelectrical Transducers.
33670269	7	48	theme	OC	749:750	arg1	voltage					753:759	Open Circuit (OC) voltage	735:759	Open Circuit (OC) voltage	735:759	Generating sensors can produce either Open Circuit (OC) voltage or Short Circuit (SC) current.
33670269	3	49	theme	mechanical	346:355	arg1	properties					372:381	significant mechanical and electrical properties	334:381	significant mechanical and electrical properties	334:381	Bacterial Cellulose (BC) is a green biopolymer, with significant mechanical and electrical properties.
33670269	13	50	theme	dramatic	1469:1476	arg1	changes					1478:1484	dramatic changes	1469:1484	dramatic changes	1469:1484	On the contrary, dramatic changes are observed for the case of SC current, whose value increases by about two orders of magnitude.
33670269	2	51	theme	Biopolymer-derived	206:223	arg1	solution					253:260	a meaningful solution	240:260	a meaningful solution to such a needing	240:278	Biopolymer-derived composites are a meaningful solution to such a needing.
33670269	2	51	theme	Biopolymer-derived	206:223	arg1	composites					225:234	Biopolymer-derived composites	206:234	Biopolymer-derived composites	206:234	Biopolymer-derived composites are a meaningful solution to such a needing.
33670269	2	52	theme	needing	272:278	arg1	a					270:270	such a needing	265:278	such a needing	265:278	Biopolymer-derived composites are a meaningful solution to such a needing.
33670269	1	53	theme	Green	133:137	arg1	sensors					139:145	Green sensors	133:145	Green sensors	133:145	Green sensors are required for the realization of a sustainable economy.
33670269	7	54	theme	Circuit	740:746	arg1	voltage					753:759	Open Circuit (OC) voltage	735:759	Open Circuit (OC) voltage	735:759	Generating sensors can produce either Open Circuit (OC) voltage or Short Circuit (SC) current.
33670269	12	55	theme	IL	1414:1415	arg1	use					1407:1409	the use	1403:1409	the use of IL	1403:1415	Reported results show that no relevant changes can be obtained because of the use of IL when the OC voltage is considered.
33670269	3	56	with	biopolymer	317:326	arg1	properties					372:381	significant mechanical and electrical properties	334:381	significant mechanical and electrical properties	334:381	Bacterial Cellulose (BC) is a green biopolymer, with significant mechanical and electrical properties.
33670269	5	57	theme	Ionic	535:539	arg1	ILs					550:552	ILs	550:552	ILs	550:552	The transductors consist of a sheet of BC, impregnated of Ionic Liquids (ILs), and covered with two layers of Conducting Polymer (CP) as the electrodes.
33670269	5	57	theme	Ionic	535:539	arg1	Liquids					541:547	Ionic Liquids	535:547	Ionic Liquids (ILs)	535:553	The transductors consist of a sheet of BC, impregnated of Ionic Liquids (ILs), and covered with two layers of Conducting Polymer (CP) as the electrodes.
33670269	9	58	theme	ILs	971:973	arg1	influence					958:966	The influence	954:966	The influence of ILs in the transduction performance, both in the case of OC voltage and SC current	954:1052	The influence of ILs in the transduction performance, both in the case of OC voltage and SC current is investigated.
33670269	10	59	theme	chemical	1114:1121	arg1	properties					1159:1168	structural, chemical, and mechanoelectrical transduction properties	1102:1168	structural, chemical, and mechanoelectrical transduction properties	1102:1168	Experimental investigations of structural, chemical, and mechanoelectrical transduction properties, when the composite is dynamically bent, are performed.
33670269	0	60	theme	Output	50:55	arg1	Signal					57:62	the Output Signal	46:62	the Output Signal	46:62	Investigation on the Role of Ionic Liquids in the Output Signal Produced by Bacterial Cellulose-Based Mechanoelectrical Transducers.
33670269	4	61	theme	mechanoelectrical	445:461	arg1	transductors					463:474	mechanoelectrical transductors	445:474	mechanoelectrical transductors	445:474	BC-based composites have been proposed to realize generating mechanoelectrical transductors.
33670269	2	62	theme	such	265:268	arg1	a					270:270	such a needing	265:278	such a needing	265:278	Biopolymer-derived composites are a meaningful solution to such a needing.
33670269	5	63	theme	Liquids	541:547	arg1	impregnated					520:530	impregnated	520:530	impregnated	520:530	The transductors consist of a sheet of BC, impregnated of Ionic Liquids (ILs), and covered with two layers of Conducting Polymer (CP) as the electrodes.
33670269	10	64	theme	mechanoelectrical	1128:1144	arg1	properties					1159:1168	structural, chemical, and mechanoelectrical transduction properties	1102:1168	structural, chemical, and mechanoelectrical transduction properties	1102:1168	Experimental investigations of structural, chemical, and mechanoelectrical transduction properties, when the composite is dynamically bent, are performed.
33670269	0	65	from	Role	21:24	arg1	Signal					57:62	the Output Signal	46:62	the Output Signal	46:62	Investigation on the Role of Ionic Liquids in the Output Signal Produced by Bacterial Cellulose-Based Mechanoelectrical Transducers.
33670269	10	66	theme	structural	1102:1111	arg1	properties					1159:1168	structural, chemical, and mechanoelectrical transduction properties	1102:1168	structural, chemical, and mechanoelectrical transduction properties	1102:1168	Experimental investigations of structural, chemical, and mechanoelectrical transduction properties, when the composite is dynamically bent, are performed.
33670269	7	67	theme	Open	735:738	arg1	voltage					753:759	Open Circuit (OC) voltage	735:759	Open Circuit (OC) voltage	735:759	Generating sensors can produce either Open Circuit (OC) voltage or Short Circuit (SC) current.
33879638	0	0	theme	Polysaccharides	72:86	arg1	Structures					33:42	Structures	33:42	Structures	33:42	Carbon Source Affects Synthesis, Structures, and Activities of Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus.
33879638	0	0	theme	Polysaccharides	72:86	arg1	Activities					49:58	Activities	49:58	Activities of Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus	49:126	Carbon Source Affects Synthesis, Structures, and Activities of Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus.
33879638	0	0	theme	Polysaccharides	72:86	arg1	Synthesis					22:30	Synthesis	22:30	Synthesis	22:30	Carbon Source Affects Synthesis, Structures, and Activities of Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus.
33879638	2	1	theme	other	495:499	arg1	groups					501:506	other groups	495:506	other groups	495:506	After 12-d fermentation, mycelial biomass, polysaccharide yield, and polysaccharide content were significantly higher in Glc+Lac group (glucose and lactose used as combined carbon source) than in other groups.
33879638	7	2	dep	in	1059:1060	arg1	vitro					1062:1066	vitro	1062:1066	vitro	1062:1066	In in vitro cell proliferation assays, viability of RAW264.7 macrophages was more strongly enhanced by NIOPL or NIOPGL than by NIOPG or NIOPF, and proliferation of HeLa or S180 tumor cells was more strongly inhibited by NIOPG or NIOPGL than by NIOPF or NIOPL, indicating that immune-enhancing and anti-tumor activities of NIOPs were substantially affected by carbon source.
33879638	1	3	theme	carbon	152:157	arg1	sources					159:165	various carbon sources	144:165	various carbon sources	144:165	The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated.
33879638	0	4	theme	Mycelial	63:70	arg1	Polysaccharides					72:86	Mycelial Polysaccharides	63:86	Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus	63:126	Carbon Source Affects Synthesis, Structures, and Activities of Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus.
33879638	7	5	theme	immune-enhancing	1332:1347	arg1	activities					1364:1373	immune-enhancing and anti-tumor activities	1332:1373	immune-enhancing and anti-tumor activities of NIOPs	1332:1382	In in vitro cell proliferation assays, viability of RAW264.7 macrophages was more strongly enhanced by NIOPL or NIOPGL than by NIOPG or NIOPF, and proliferation of HeLa or S180 tumor cells was more strongly inhibited by NIOPG or NIOPGL than by NIOPF or NIOPL, indicating that immune-enhancing and anti-tumor activities of NIOPs were substantially affected by carbon source.
33879638	1	6	dep	fungus	232:237	arg1	Inonotus					239:246	Inonotus	239:246	Inonotus	239:246	The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated.
33879638	1	7	theme	sources	159:165	arg1	effects					133:139	The effects	129:139	The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation	129:278	The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated.
33879638	1	8	theme	liquid	260:265	arg1	fermentation					267:278	liquid fermentation	260:278	liquid fermentation	260:278	The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated.
33879638	0	9	theme	Medicinal	93:101	arg1	obliquus					119:126	Medicinal Fungus Inonotus obliquus	93:126	Medicinal Fungus Inonotus obliquus	93:126	Carbon Source Affects Synthesis, Structures, and Activities of Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus.
33879638	9	10	theme	I.	1767:1768	arg1	polysaccharides					1779:1793	I. obliquus polysaccharides	1767:1793	I. obliquus polysaccharides	1767:1793	Our findings, taken together, clearly demonstrate that carbon source plays an essential role in determining structure and activities of I. obliquus polysaccharides by regulating expression of key genes in polysaccharide biosynthetic pathway.
33879638	2	11	theme	12-d	305:308	arg1	fermentation					310:321	12-d fermentation	305:321	12-d fermentation	305:321	After 12-d fermentation, mycelial biomass, polysaccharide yield, and polysaccharide content were significantly higher in Glc+Lac group (glucose and lactose used as combined carbon source) than in other groups.
33879638	6	12	theme	differing	1033:1041	arg1	proportions					1043:1053	differing proportions	1033:1053	differing proportions	1033:1053	The NIOPs were classified as α-type heteropolysaccharides with 1→2, 1→3, 1→4, 1→6 linkages in differing proportions.
33879638	8	13	theme	4-epimerase	1525:1535	arg1	levels					1472:1477	expression levels	1461:1477	expression levels of phosphoglucose isomerase (PGI) and UDP-Glc 4-epimerase (UGE), two key genes involved in polysaccharide synthesis,	1461:1594	qRT-PCR analysis revealed that expression levels of phosphoglucose isomerase (PGI) and UDP-Glc 4-epimerase (UGE), two key genes involved in polysaccharide synthesis, varied depending on carbon source.
33879638	3	14	theme	derivative	547:556	arg1	polysaccharides					566:580	the derivative neutral polysaccharides	543:580	the derivative neutral polysaccharides (NIOPs)	543:588	Crude polysaccharides (CIOPs) and the derivative neutral polysaccharides (NIOPs) were obtained from mycelia fermented using Glc, fructose (Fru), Lac, or Glc+Lac as carbon source.
33879638	3	14	theme	derivative	547:556	arg1	NIOPs					583:587	NIOPs	583:587	NIOPs	583:587	Crude polysaccharides (CIOPs) and the derivative neutral polysaccharides (NIOPs) were obtained from mycelia fermented using Glc, fructose (Fru), Lac, or Glc+Lac as carbon source.
33879638	2	15	theme	polysaccharide	368:381	arg1	content					383:389	polysaccharide content	368:389	polysaccharide content	368:389	After 12-d fermentation, mycelial biomass, polysaccharide yield, and polysaccharide content were significantly higher in Glc+Lac group (glucose and lactose used as combined carbon source) than in other groups.
33879638	8	16	theme	polysaccharide	1570:1583	arg1	synthesis					1585:1593	polysaccharide synthesis	1570:1593	polysaccharide synthesis	1570:1593	qRT-PCR analysis revealed that expression levels of phosphoglucose isomerase (PGI) and UDP-Glc 4-epimerase (UGE), two key genes involved in polysaccharide synthesis, varied depending on carbon source.
33879638	8	17	theme	UDP-Glc	1517:1523	arg1	4-epimerase					1525:1535	UDP-Glc 4-epimerase	1517:1535	UDP-Glc 4-epimerase	1517:1535	qRT-PCR analysis revealed that expression levels of phosphoglucose isomerase (PGI) and UDP-Glc 4-epimerase (UGE), two key genes involved in polysaccharide synthesis, varied depending on carbon source.
33879638	0	18	theme	Inonotus	110:117	arg1	obliquus					119:126	Medicinal Fungus Inonotus obliquus	93:126	Medicinal Fungus Inonotus obliquus	93:126	Carbon Source Affects Synthesis, Structures, and Activities of Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus.
33879638	9	19	theme	obliquus	1770:1777	arg1	polysaccharides					1779:1793	I. obliquus polysaccharides	1767:1793	I. obliquus polysaccharides	1767:1793	Our findings, taken together, clearly demonstrate that carbon source plays an essential role in determining structure and activities of I. obliquus polysaccharides by regulating expression of key genes in polysaccharide biosynthetic pathway.
33879638	9	20	theme	polysaccharides	1779:1793	arg1	activities					1753:1762	activities	1753:1762	activities	1753:1762	Our findings, taken together, clearly demonstrate that carbon source plays an essential role in determining structure and activities of I. obliquus polysaccharides by regulating expression of key genes in polysaccharide biosynthetic pathway.
33879638	9	20	theme	polysaccharides	1779:1793	arg1	structure					1739:1747	structure	1739:1747	structure	1739:1747	Our findings, taken together, clearly demonstrate that carbon source plays an essential role in determining structure and activities of I. obliquus polysaccharides by regulating expression of key genes in polysaccharide biosynthetic pathway.
33879638	2	21	theme	Glc+Lac	420:426	arg1	group					428:432	Glc+Lac group	420:432	Glc+Lac group (glucose and lactose used as combined carbon source)	420:485	After 12-d fermentation, mycelial biomass, polysaccharide yield, and polysaccharide content were significantly higher in Glc+Lac group (glucose and lactose used as combined carbon source) than in other groups.
33879638	0	22	theme	Fungus	103:108	arg1	obliquus					119:126	Medicinal Fungus Inonotus obliquus	93:126	Medicinal Fungus Inonotus obliquus	93:126	Carbon Source Affects Synthesis, Structures, and Activities of Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus.
33879638	0	23	from	Structures	33:42	arg1	obliquus					119:126	Medicinal Fungus Inonotus obliquus	93:126	Medicinal Fungus Inonotus obliquus	93:126	Carbon Source Affects Synthesis, Structures, and Activities of Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus.
33879638	7	24	theme	proliferation	1073:1085	arg1	assays					1087:1092	in vitro cell proliferation assays	1059:1092	in vitro cell proliferation assays	1059:1092	In in vitro cell proliferation assays, viability of RAW264.7 macrophages was more strongly enhanced by NIOPL or NIOPGL than by NIOPG or NIOPF, and proliferation of HeLa or S180 tumor cells was more strongly inhibited by NIOPG or NIOPGL than by NIOPF or NIOPL, indicating that immune-enhancing and anti-tumor activities of NIOPs were substantially affected by carbon source.
33879638	9	25	theme	key	1823:1825	arg1	genes					1827:1831	key genes	1823:1831	key genes	1823:1831	Our findings, taken together, clearly demonstrate that carbon source plays an essential role in determining structure and activities of I. obliquus polysaccharides by regulating expression of key genes in polysaccharide biosynthetic pathway.
33879638	1	26	theme	mycelial	170:177	arg1	growth					179:184	mycelial growth	170:184	mycelial growth	170:184	The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated.
33879638	2	27	theme	combined	463:470	arg1	source					479:484	combined carbon source	463:484	combined carbon source	463:484	After 12-d fermentation, mycelial biomass, polysaccharide yield, and polysaccharide content were significantly higher in Glc+Lac group (glucose and lactose used as combined carbon source) than in other groups.
33879638	7	28	theme	macrophages	1117:1127	arg1	viability					1095:1103	viability	1095:1103	viability of RAW264.7 macrophages	1095:1127	In in vitro cell proliferation assays, viability of RAW264.7 macrophages was more strongly enhanced by NIOPL or NIOPGL than by NIOPG or NIOPF, and proliferation of HeLa or S180 tumor cells was more strongly inhibited by NIOPG or NIOPGL than by NIOPF or NIOPL, indicating that immune-enhancing and anti-tumor activities of NIOPs were substantially affected by carbon source.
33879638	7	29	theme	anti-tumor	1353:1362	arg1	activities					1364:1373	immune-enhancing and anti-tumor activities	1332:1373	immune-enhancing and anti-tumor activities of NIOPs	1332:1382	In in vitro cell proliferation assays, viability of RAW264.7 macrophages was more strongly enhanced by NIOPL or NIOPGL than by NIOPG or NIOPF, and proliferation of HeLa or S180 tumor cells was more strongly inhibited by NIOPG or NIOPGL than by NIOPF or NIOPL, indicating that immune-enhancing and anti-tumor activities of NIOPs were substantially affected by carbon source.
33879638	1	30	from	effects	133:139	arg1	growth					179:184	mycelial growth	170:184	mycelial growth	170:184	The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated.
33879638	1	30	from	effects	133:139	arg1	synthesis					205:213	polysaccharide synthesis	190:213	polysaccharide synthesis	190:213	The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated.
33879638	8	31	theme	isomerase	1497:1505	arg1	levels					1472:1477	expression levels	1461:1477	expression levels of phosphoglucose isomerase (PGI) and UDP-Glc 4-epimerase (UGE), two key genes involved in polysaccharide synthesis,	1461:1594	qRT-PCR analysis revealed that expression levels of phosphoglucose isomerase (PGI) and UDP-Glc 4-epimerase (UGE), two key genes involved in polysaccharide synthesis, varied depending on carbon source.
33879638	7	32	theme	cell	1068:1071	arg1	assays					1087:1092	in vitro cell proliferation assays	1059:1092	in vitro cell proliferation assays	1059:1092	In in vitro cell proliferation assays, viability of RAW264.7 macrophages was more strongly enhanced by NIOPL or NIOPGL than by NIOPG or NIOPF, and proliferation of HeLa or S180 tumor cells was more strongly inhibited by NIOPG or NIOPGL than by NIOPF or NIOPL, indicating that immune-enhancing and anti-tumor activities of NIOPs were substantially affected by carbon source.
33879638	0	33	theme	Carbon	0:5	arg1	Source					7:12	Carbon Source	0:12	Carbon Source	0:12	Carbon Source Affects Synthesis, Structures, and Activities of Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus.
33879638	8	34	theme	qRT-PCR	1430:1436	arg1	analysis					1438:1445	qRT-PCR analysis	1430:1445	qRT-PCR analysis	1430:1445	qRT-PCR analysis revealed that expression levels of phosphoglucose isomerase (PGI) and UDP-Glc 4-epimerase (UGE), two key genes involved in polysaccharide synthesis, varied depending on carbon source.
33879638	2	35	theme	mycelial	324:331	arg1	biomass					333:339	mycelial biomass	324:339	mycelial biomass	324:339	After 12-d fermentation, mycelial biomass, polysaccharide yield, and polysaccharide content were significantly higher in Glc+Lac group (glucose and lactose used as combined carbon source) than in other groups.
33879638	5	36	theme	Monosaccharide	821:834	arg1	composition					836:846	Monosaccharide composition	821:846	Monosaccharide composition analyses	821:855	Monosaccharide composition analyses revealed that NIOPs were composed of Glc, Man, and Gal at different molar ratios.
33879638	7	37	theme	in	1059:1060	arg1	assays					1087:1092	in vitro cell proliferation assays	1059:1092	in vitro cell proliferation assays	1059:1092	In in vitro cell proliferation assays, viability of RAW264.7 macrophages was more strongly enhanced by NIOPL or NIOPGL than by NIOPG or NIOPF, and proliferation of HeLa or S180 tumor cells was more strongly inhibited by NIOPG or NIOPGL than by NIOPF or NIOPL, indicating that immune-enhancing and anti-tumor activities of NIOPs were substantially affected by carbon source.
33879638	1	38	from	fermentation	267:278	arg1	growth					179:184	mycelial growth	170:184	mycelial growth	170:184	The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated.
33879638	1	38	from	fermentation	267:278	arg1	synthesis					205:213	polysaccharide synthesis	190:213	polysaccharide synthesis	190:213	The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated.
33879638	7	39	theme	S180	1228:1231	arg1	cells					1239:1243	HeLa or S180 tumor cells	1220:1243	HeLa or S180 tumor cells	1220:1243	In in vitro cell proliferation assays, viability of RAW264.7 macrophages was more strongly enhanced by NIOPL or NIOPGL than by NIOPG or NIOPF, and proliferation of HeLa or S180 tumor cells was more strongly inhibited by NIOPG or NIOPGL than by NIOPF or NIOPL, indicating that immune-enhancing and anti-tumor activities of NIOPs were substantially affected by carbon source.
33879638	3	40	theme	neutral	558:564	arg1	polysaccharides					566:580	the derivative neutral polysaccharides	543:580	the derivative neutral polysaccharides (NIOPs)	543:588	Crude polysaccharides (CIOPs) and the derivative neutral polysaccharides (NIOPs) were obtained from mycelia fermented using Glc, fructose (Fru), Lac, or Glc+Lac as carbon source.
33879638	3	40	theme	neutral	558:564	arg1	NIOPs					583:587	NIOPs	583:587	NIOPs	583:587	Crude polysaccharides (CIOPs) and the derivative neutral polysaccharides (NIOPs) were obtained from mycelia fermented using Glc, fructose (Fru), Lac, or Glc+Lac as carbon source.
33879638	1	41	theme	polysaccharide	190:203	arg1	synthesis					205:213	polysaccharide synthesis	190:213	polysaccharide synthesis	190:213	The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated.
33879638	9	42	theme	carbon	1686:1691	arg1	source					1693:1698	carbon source	1686:1698	carbon source	1686:1698	Our findings, taken together, clearly demonstrate that carbon source plays an essential role in determining structure and activities of I. obliquus polysaccharides by regulating expression of key genes in polysaccharide biosynthetic pathway.
33879638	6	43	with	heteropolysaccharides	975:995	arg1	1→4					1012:1014	1→4	1012:1014	1→4	1012:1014	The NIOPs were classified as α-type heteropolysaccharides with 1→2, 1→3, 1→4, 1→6 linkages in differing proportions.
33879638	6	43	with	heteropolysaccharides	975:995	arg1	linkages					1021:1028	linkages	1021:1028	linkages in differing proportions	1021:1053	The NIOPs were classified as α-type heteropolysaccharides with 1→2, 1→3, 1→4, 1→6 linkages in differing proportions.
33879638	6	43	with	heteropolysaccharides	975:995	arg1	1→3					1007:1009	1→3	1007:1009	1→3	1007:1009	The NIOPs were classified as α-type heteropolysaccharides with 1→2, 1→3, 1→4, 1→6 linkages in differing proportions.
33879638	6	43	with	heteropolysaccharides	975:995	arg1	1→2					1002:1004	1→2	1002:1004	1→2	1002:1004	The NIOPs were classified as α-type heteropolysaccharides with 1→2, 1→3, 1→4, 1→6 linkages in differing proportions.
33879638	4	44	theme	NIOPs	714:718	arg1	weights					698:704	Molecular weights	688:704	Molecular weights of four NIOPs (termed as NIOPG, NIOPF, NIOPL, and NIOPGL)	688:762	Molecular weights of four NIOPs (termed as NIOPG, NIOPF, NIOPL, and NIOPGL) were respectively 780.90, 1105.00, 25.32, and 10.28 kDa.
33879638	8	45	theme	carbon	1616:1621	arg1	source					1623:1628	carbon source	1616:1628	carbon source	1616:1628	qRT-PCR analysis revealed that expression levels of phosphoglucose isomerase (PGI) and UDP-Glc 4-epimerase (UGE), two key genes involved in polysaccharide synthesis, varied depending on carbon source.
33879638	3	46	theme	Crude	509:513	arg1	polysaccharides					515:529	Crude polysaccharides	509:529	Crude polysaccharides (CIOPs)	509:537	Crude polysaccharides (CIOPs) and the derivative neutral polysaccharides (NIOPs) were obtained from mycelia fermented using Glc, fructose (Fru), Lac, or Glc+Lac as carbon source.
33879638	3	46	theme	Crude	509:513	arg1	CIOPs					532:536	CIOPs	532:536	CIOPs	532:536	Crude polysaccharides (CIOPs) and the derivative neutral polysaccharides (NIOPs) were obtained from mycelia fermented using Glc, fructose (Fru), Lac, or Glc+Lac as carbon source.
33879638	7	47	theme	carbon	1415:1420	arg1	source					1422:1427	carbon source	1415:1427	carbon source	1415:1427	In in vitro cell proliferation assays, viability of RAW264.7 macrophages was more strongly enhanced by NIOPL or NIOPGL than by NIOPG or NIOPF, and proliferation of HeLa or S180 tumor cells was more strongly inhibited by NIOPG or NIOPGL than by NIOPF or NIOPL, indicating that immune-enhancing and anti-tumor activities of NIOPs were substantially affected by carbon source.
33879638	5	48	theme	molar	925:929	arg1	ratios					931:936	different molar ratios	915:936	different molar ratios	915:936	Monosaccharide composition analyses revealed that NIOPs were composed of Glc, Man, and Gal at different molar ratios.
33879638	6	49	theme	α-type	968:973	arg1	heteropolysaccharides					975:995	α-type heteropolysaccharides	968:995	α-type heteropolysaccharides with 1→2, 1→3, 1→4, 1→6 linkages in differing proportions	968:1053	The NIOPs were classified as α-type heteropolysaccharides with 1→2, 1→3, 1→4, 1→6 linkages in differing proportions.
33879638	6	49	theme	α-type	968:973	arg1	NIOPs					943:947	The NIOPs	939:947	The NIOPs	939:947	The NIOPs were classified as α-type heteropolysaccharides with 1→2, 1→3, 1→4, 1→6 linkages in differing proportions.
33879638	7	50	theme	RAW264.7	1108:1115	arg1	macrophages					1117:1127	RAW264.7 macrophages	1108:1127	RAW264.7 macrophages	1108:1127	In in vitro cell proliferation assays, viability of RAW264.7 macrophages was more strongly enhanced by NIOPL or NIOPGL than by NIOPG or NIOPF, and proliferation of HeLa or S180 tumor cells was more strongly inhibited by NIOPG or NIOPGL than by NIOPF or NIOPL, indicating that immune-enhancing and anti-tumor activities of NIOPs were substantially affected by carbon source.
33879638	8	51	theme	expression	1461:1470	arg1	levels					1472:1477	expression levels	1461:1477	expression levels of phosphoglucose isomerase (PGI) and UDP-Glc 4-epimerase (UGE), two key genes involved in polysaccharide synthesis,	1461:1594	qRT-PCR analysis revealed that expression levels of phosphoglucose isomerase (PGI) and UDP-Glc 4-epimerase (UGE), two key genes involved in polysaccharide synthesis, varied depending on carbon source.
33879638	2	52	dep	group	428:432	arg1	lactose					447:453	lactose	447:453	lactose	447:453	After 12-d fermentation, mycelial biomass, polysaccharide yield, and polysaccharide content were significantly higher in Glc+Lac group (glucose and lactose used as combined carbon source) than in other groups.
33879638	2	52	dep	group	428:432	arg1	glucose					435:441	glucose	435:441	glucose	435:441	After 12-d fermentation, mycelial biomass, polysaccharide yield, and polysaccharide content were significantly higher in Glc+Lac group (glucose and lactose used as combined carbon source) than in other groups.
33879638	8	53	theme	phosphoglucose	1482:1495	arg1	PGI					1508:1510	PGI	1508:1510	PGI	1508:1510	qRT-PCR analysis revealed that expression levels of phosphoglucose isomerase (PGI) and UDP-Glc 4-epimerase (UGE), two key genes involved in polysaccharide synthesis, varied depending on carbon source.
33879638	8	53	theme	phosphoglucose	1482:1495	arg1	genes					1552:1556	two key genes	1544:1556	two key genes involved in polysaccharide synthesis	1544:1593	qRT-PCR analysis revealed that expression levels of phosphoglucose isomerase (PGI) and UDP-Glc 4-epimerase (UGE), two key genes involved in polysaccharide synthesis, varied depending on carbon source.
33879638	8	53	theme	phosphoglucose	1482:1495	arg1	isomerase					1497:1505	phosphoglucose isomerase	1482:1505	phosphoglucose isomerase (PGI)	1482:1511	qRT-PCR analysis revealed that expression levels of phosphoglucose isomerase (PGI) and UDP-Glc 4-epimerase (UGE), two key genes involved in polysaccharide synthesis, varied depending on carbon source.
33879638	8	53	theme	phosphoglucose	1482:1495	arg1	UGE					1538:1540	UGE	1538:1540	UGE	1538:1540	qRT-PCR analysis revealed that expression levels of phosphoglucose isomerase (PGI) and UDP-Glc 4-epimerase (UGE), two key genes involved in polysaccharide synthesis, varied depending on carbon source.
33879638	0	54	from	Activities	49:58	arg1	obliquus					119:126	Medicinal Fungus Inonotus obliquus	93:126	Medicinal Fungus Inonotus obliquus	93:126	Carbon Source Affects Synthesis, Structures, and Activities of Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus.
33879638	9	55	from	expression	1809:1818	arg1	pathway					1864:1870	polysaccharide biosynthetic pathway	1836:1870	polysaccharide biosynthetic pathway	1836:1870	Our findings, taken together, clearly demonstrate that carbon source plays an essential role in determining structure and activities of I. obliquus polysaccharides by regulating expression of key genes in polysaccharide biosynthetic pathway.
33879638	9	56	theme	polysaccharide	1836:1849	arg1	pathway					1864:1870	polysaccharide biosynthetic pathway	1836:1870	polysaccharide biosynthetic pathway	1836:1870	Our findings, taken together, clearly demonstrate that carbon source plays an essential role in determining structure and activities of I. obliquus polysaccharides by regulating expression of key genes in polysaccharide biosynthetic pathway.
33879638	5	57	theme	composition	836:846	arg1	analyses					848:855	Monosaccharide composition analyses	821:855	Monosaccharide composition analyses	821:855	Monosaccharide composition analyses revealed that NIOPs were composed of Glc, Man, and Gal at different molar ratios.
33879638	2	58	theme	carbon	472:477	arg1	source					479:484	combined carbon source	463:484	combined carbon source	463:484	After 12-d fermentation, mycelial biomass, polysaccharide yield, and polysaccharide content were significantly higher in Glc+Lac group (glucose and lactose used as combined carbon source) than in other groups.
33879638	1	59	from	obliquus	248:255	arg1	fermentation					267:278	liquid fermentation	260:278	liquid fermentation	260:278	The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated.
33879638	9	60	theme	biosynthetic	1851:1862	arg1	pathway					1864:1870	polysaccharide biosynthetic pathway	1836:1870	polysaccharide biosynthetic pathway	1836:1870	Our findings, taken together, clearly demonstrate that carbon source plays an essential role in determining structure and activities of I. obliquus polysaccharides by regulating expression of key genes in polysaccharide biosynthetic pathway.
33879638	4	61	dep	NIOPs	714:718	arg1	termed					721:726	termed	721:726	termed as NIOPG, NIOPF, NIOPL, and NIOPGL	721:761	Molecular weights of four NIOPs (termed as NIOPG, NIOPF, NIOPL, and NIOPGL) were respectively 780.90, 1105.00, 25.32, and 10.28 kDa.
33879638	9	62	theme	genes	1827:1831	arg1	expression					1809:1818	expression	1809:1818	expression of key genes in polysaccharide biosynthetic pathway	1809:1870	Our findings, taken together, clearly demonstrate that carbon source plays an essential role in determining structure and activities of I. obliquus polysaccharides by regulating expression of key genes in polysaccharide biosynthetic pathway.
33879638	5	63	theme	different	915:923	arg1	ratios					931:936	different molar ratios	915:936	different molar ratios	915:936	Monosaccharide composition analyses revealed that NIOPs were composed of Glc, Man, and Gal at different molar ratios.
33879638	7	64	theme	tumor	1233:1237	arg1	cells					1239:1243	HeLa or S180 tumor cells	1220:1243	HeLa or S180 tumor cells	1220:1243	In in vitro cell proliferation assays, viability of RAW264.7 macrophages was more strongly enhanced by NIOPL or NIOPGL than by NIOPG or NIOPF, and proliferation of HeLa or S180 tumor cells was more strongly inhibited by NIOPG or NIOPGL than by NIOPF or NIOPL, indicating that immune-enhancing and anti-tumor activities of NIOPs were substantially affected by carbon source.
33879638	1	65	from	synthesis	205:213	arg1	fermentation					267:278	liquid fermentation	260:278	liquid fermentation	260:278	The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated.
33879638	2	66	theme	polysaccharide	342:355	arg1	yield					357:361	polysaccharide yield	342:361	polysaccharide yield	342:361	After 12-d fermentation, mycelial biomass, polysaccharide yield, and polysaccharide content were significantly higher in Glc+Lac group (glucose and lactose used as combined carbon source) than in other groups.
33879638	1	67	theme	medicinal	222:230	arg1	obliquus					248:255	the medicinal fungus Inonotus obliquus	218:255	the medicinal fungus Inonotus obliquus in liquid fermentation	218:278	The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated.
33879638	6	68	from	linkages	1021:1028	arg1	proportions					1043:1053	differing proportions	1033:1053	differing proportions	1033:1053	The NIOPs were classified as α-type heteropolysaccharides with 1→2, 1→3, 1→4, 1→6 linkages in differing proportions.
33879638	7	69	theme	cells	1239:1243	arg1	proliferation					1203:1215	proliferation	1203:1215	proliferation of HeLa or S180 tumor cells	1203:1243	In in vitro cell proliferation assays, viability of RAW264.7 macrophages was more strongly enhanced by NIOPL or NIOPGL than by NIOPG or NIOPF, and proliferation of HeLa or S180 tumor cells was more strongly inhibited by NIOPG or NIOPGL than by NIOPF or NIOPL, indicating that immune-enhancing and anti-tumor activities of NIOPs were substantially affected by carbon source.
33879638	1	70	from	growth	179:184	arg1	fermentation					267:278	liquid fermentation	260:278	liquid fermentation	260:278	The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated.
33879638	4	71	theme	Molecular	688:696	arg1	weights					698:704	Molecular weights	688:704	Molecular weights of four NIOPs (termed as NIOPG, NIOPF, NIOPL, and NIOPGL)	688:762	Molecular weights of four NIOPs (termed as NIOPG, NIOPF, NIOPL, and NIOPGL) were respectively 780.90, 1105.00, 25.32, and 10.28 kDa.
33879638	1	72	theme	fungus	232:237	arg1	obliquus					248:255	the medicinal fungus Inonotus obliquus	218:255	the medicinal fungus Inonotus obliquus in liquid fermentation	218:278	The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated.
33879638	7	73	theme	NIOPs	1378:1382	arg1	activities					1364:1373	immune-enhancing and anti-tumor activities	1332:1373	immune-enhancing and anti-tumor activities of NIOPs	1332:1382	In in vitro cell proliferation assays, viability of RAW264.7 macrophages was more strongly enhanced by NIOPL or NIOPGL than by NIOPG or NIOPF, and proliferation of HeLa or S180 tumor cells was more strongly inhibited by NIOPG or NIOPGL than by NIOPF or NIOPL, indicating that immune-enhancing and anti-tumor activities of NIOPs were substantially affected by carbon source.
33879638	9	74	theme	essential	1709:1717	arg1	role					1719:1722	an essential role	1706:1722	an essential role	1706:1722	Our findings, taken together, clearly demonstrate that carbon source plays an essential role in determining structure and activities of I. obliquus polysaccharides by regulating expression of key genes in polysaccharide biosynthetic pathway.
33879638	7	75	theme	HeLa	1220:1223	arg1	cells					1239:1243	HeLa or S180 tumor cells	1220:1243	HeLa or S180 tumor cells	1220:1243	In in vitro cell proliferation assays, viability of RAW264.7 macrophages was more strongly enhanced by NIOPL or NIOPGL than by NIOPG or NIOPF, and proliferation of HeLa or S180 tumor cells was more strongly inhibited by NIOPG or NIOPGL than by NIOPF or NIOPL, indicating that immune-enhancing and anti-tumor activities of NIOPs were substantially affected by carbon source.
33879638	0	76	from	Synthesis	22:30	arg1	obliquus					119:126	Medicinal Fungus Inonotus obliquus	93:126	Medicinal Fungus Inonotus obliquus	93:126	Carbon Source Affects Synthesis, Structures, and Activities of Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus.
33879638	0	77	from	obliquus	119:126	arg1	Structures					33:42	Structures	33:42	Structures	33:42	Carbon Source Affects Synthesis, Structures, and Activities of Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus.
33879638	0	77	from	obliquus	119:126	arg1	Activities					49:58	Activities	49:58	Activities of Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus	49:126	Carbon Source Affects Synthesis, Structures, and Activities of Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus.
33879638	0	77	from	obliquus	119:126	arg1	Synthesis					22:30	Synthesis	22:30	Synthesis	22:30	Carbon Source Affects Synthesis, Structures, and Activities of Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus.
33879638	0	77	from	obliquus	119:126	arg1	Polysaccharides					72:86	Mycelial Polysaccharides	63:86	Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus	63:126	Carbon Source Affects Synthesis, Structures, and Activities of Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus.
33879638	3	78	theme	carbon	673:678	arg1	source					680:685	carbon source	673:685	carbon source	673:685	Crude polysaccharides (CIOPs) and the derivative neutral polysaccharides (NIOPs) were obtained from mycelia fermented using Glc, fructose (Fru), Lac, or Glc+Lac as carbon source.
33879638	1	79	theme	various	144:150	arg1	sources					159:165	various carbon sources	144:165	various carbon sources	144:165	The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated.
33879638	8	80	theme	key	1548:1550	arg1	genes					1552:1556	two key genes	1544:1556	two key genes involved in polysaccharide synthesis	1544:1593	qRT-PCR analysis revealed that expression levels of phosphoglucose isomerase (PGI) and UDP-Glc 4-epimerase (UGE), two key genes involved in polysaccharide synthesis, varied depending on carbon source.
33879638	8	80	theme	key	1548:1550	arg1	isomerase					1497:1505	phosphoglucose isomerase	1482:1505	phosphoglucose isomerase (PGI)	1482:1511	qRT-PCR analysis revealed that expression levels of phosphoglucose isomerase (PGI) and UDP-Glc 4-epimerase (UGE), two key genes involved in polysaccharide synthesis, varied depending on carbon source.
33879638	1	81	theme	obliquus	248:255	arg1	growth					179:184	mycelial growth	170:184	mycelial growth	170:184	The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated.
33879638	1	81	theme	obliquus	248:255	arg1	synthesis					205:213	polysaccharide synthesis	190:213	polysaccharide synthesis	190:213	The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated.
33445487	0	0	theme	gB	91:92	arg1	Function					47:54	Function	47:54	Function	47:54	Influence of N-glycosylation on Expression and Function of Pseudorabies Virus Glycoprotein gB.
33445487	0	0	theme	gB	91:92	arg1	Expression					32:41	Expression	32:41	Expression	32:41	Influence of N-glycosylation on Expression and Function of Pseudorabies Virus Glycoprotein gB.
33445487	10	1	from	functional	1301:1310	arg1	fusion					1325:1330	cell-cell fusion	1315:1330	cell-cell fusion	1315:1330	Although all single mutants were functional in cell-cell fusion and viral entry, simultaneous inactivation of all six N-glycosylation sites severely impaired fusion activity and viral entry, suggesting a critical role of N-glycans for maintaining gB structure and function.
33445487	10	1	from	functional	1301:1310	arg1	entry					1342:1346	viral entry	1336:1346	viral entry	1336:1346	Although all single mutants were functional in cell-cell fusion and viral entry, simultaneous inactivation of all six N-glycosylation sites severely impaired fusion activity and viral entry, suggesting a critical role of N-glycans for maintaining gB structure and function.
33445487	9	2	theme	gB	1139:1140	arg1	expression					1142:1151	gB expression	1139:1151	gB expression	1139:1151	However, while glycosylation at most sites was dispensable for gB expression and fusogenicity, inactivation of N154 and N700 affected gB processing by furin cleavage and surface localization.
33445487	10	3	theme	gB	1515:1516	arg1	structure					1518:1526	gB structure	1515:1526	gB structure	1515:1526	Although all single mutants were functional in cell-cell fusion and viral entry, simultaneous inactivation of all six N-glycosylation sites severely impaired fusion activity and viral entry, suggesting a critical role of N-glycans for maintaining gB structure and function.
33445487	4	4	theme	N-linked	556:563	arg1	glycosylation					565:577	N-linked glycosylation	556:577	N-linked glycosylation	556:577	gB of the alphaherpesvirus pseudorabies virus (PrV) contains six consensus sites for N-linked glycosylation, but their functional relevance is unknown.
33445487	0	5	theme	Glycoprotein	78:89	arg1	gB					91:92	Pseudorabies Virus Glycoprotein gB	59:92	Pseudorabies Virus Glycoprotein gB	59:92	Influence of N-glycosylation on Expression and Function of Pseudorabies Virus Glycoprotein gB.
33445487	4	6	theme	pseudorabies	498:509	arg1	PrV					518:520	PrV	518:520	PrV	518:520	gB of the alphaherpesvirus pseudorabies virus (PrV) contains six consensus sites for N-linked glycosylation, but their functional relevance is unknown.
33445487	4	6	theme	pseudorabies	498:509	arg1	virus					511:515	the alphaherpesvirus pseudorabies virus	477:515	the alphaherpesvirus pseudorabies virus (PrV)	477:521	gB of the alphaherpesvirus pseudorabies virus (PrV) contains six consensus sites for N-linked glycosylation, but their functional relevance is unknown.
33445487	10	7	theme	cell-cell	1315:1323	arg1	fusion					1325:1330	cell-cell fusion	1315:1330	cell-cell fusion	1315:1330	Although all single mutants were functional in cell-cell fusion and viral entry, simultaneous inactivation of all six N-glycosylation sites severely impaired fusion activity and viral entry, suggesting a critical role of N-glycans for maintaining gB structure and function.
33445487	9	8	theme	N700	1196:1199	arg1	inactivation					1171:1182	inactivation	1171:1182	inactivation of N154 and N700	1171:1199	However, while glycosylation at most sites was dispensable for gB expression and fusogenicity, inactivation of N154 and N700 affected gB processing by furin cleavage and surface localization.
33445487	5	9	theme	PrV	712:714	arg1	gB					716:717	PrV gB	712:717	PrV gB	712:717	Here, we investigated the occupancy and functional relevance of N-glycosylation sites in PrV gB.
33445487	0	10	from	Influence	0:8	arg1	Function					47:54	Function	47:54	Function	47:54	Influence of N-glycosylation on Expression and Function of Pseudorabies Virus Glycoprotein gB.
33445487	0	10	from	Influence	0:8	arg1	Expression					32:41	Expression	32:41	Expression	32:41	Influence of N-glycosylation on Expression and Function of Pseudorabies Virus Glycoprotein gB.
33445487	10	11	theme	fusion	1426:1431	arg1	activity					1433:1440	fusion activity	1426:1440	fusion activity	1426:1440	Although all single mutants were functional in cell-cell fusion and viral entry, simultaneous inactivation of all six N-glycosylation sites severely impaired fusion activity and viral entry, suggesting a critical role of N-glycans for maintaining gB structure and function.
33445487	7	12	theme	cell-cell	941:949	arg1	assays					958:963	cell-cell fusion assays	941:963	cell-cell fusion assays	941:963	The resulting proteins were tested for expression, fusion activity in cell-cell fusion assays and complementation of a gB-deficient PrV mutant.
33445487	2	13	theme	envelope	278:285	arg1	proteins					294:301	all viral envelope fusion proteins	268:301	all viral envelope fusion proteins	268:301	Like all viral envelope fusion proteins, gB is modified by asparagine (N)-linked glycosylation.
33445487	10	14	gly	N-glycosylation	1386:1400	arg2	sites					1402:1406	all six N-glycosylation sites	1378:1406	all six N-glycosylation sites	1378:1406	Although all single mutants were functional in cell-cell fusion and viral entry, simultaneous inactivation of all six N-glycosylation sites severely impaired fusion activity and viral entry, suggesting a critical role of N-glycans for maintaining gB structure and function.
33445487	10	14	gly	N-glycosylation	1386:1400	arg2	six					1382:1384	six	1382:1384	six	1382:1384	Although all single mutants were functional in cell-cell fusion and viral entry, simultaneous inactivation of all six N-glycosylation sites severely impaired fusion activity and viral entry, suggesting a critical role of N-glycans for maintaining gB structure and function.
33445487	7	15	from	activity	929:936	arg1	assays					958:963	cell-cell fusion assays	941:963	cell-cell fusion assays	941:963	The resulting proteins were tested for expression, fusion activity in cell-cell fusion assays and complementation of a gB-deficient PrV mutant.
33445487	7	16	theme	fusion	922:927	arg1	activity					929:936	fusion activity	922:936	fusion activity in cell-cell fusion assays	922:963	The resulting proteins were tested for expression, fusion activity in cell-cell fusion assays and complementation of a gB-deficient PrV mutant.
33445487	2	17	theme	viral	272:276	arg1	proteins					294:301	all viral envelope fusion proteins	268:301	all viral envelope fusion proteins	268:301	Like all viral envelope fusion proteins, gB is modified by asparagine (N)-linked glycosylation.
33445487	5	18	dep	occupancy	649:657	arg1	the					645:647	the	645:647	the	645:647	Here, we investigated the occupancy and functional relevance of N-glycosylation sites in PrV gB.
33445487	9	19	from	sites	1113:1117	arg1	glycosylation					1091:1103	glycosylation	1091:1103	glycosylation at most sites	1091:1117	However, while glycosylation at most sites was dispensable for gB expression and fusogenicity, inactivation of N154 and N700 affected gB processing by furin cleavage and surface localization.
33445487	10	20	theme	N-glycans	1489:1497	arg1	role					1481:1484	a critical role	1470:1484	a critical role of N-glycans for maintaining gB structure and function	1470:1539	Although all single mutants were functional in cell-cell fusion and viral entry, simultaneous inactivation of all six N-glycosylation sites severely impaired fusion activity and viral entry, suggesting a critical role of N-glycans for maintaining gB structure and function.
33445487	7	21	from	expression	910:919	arg1	assays					958:963	cell-cell fusion assays	941:963	cell-cell fusion assays	941:963	The resulting proteins were tested for expression, fusion activity in cell-cell fusion assays and complementation of a gB-deficient PrV mutant.
33445487	1	22	gly	glycoprotein	104:115	arg1	g					118:118	g	118:118	g	118:118	Envelope glycoprotein (g)B is conserved throughout the Herpesviridae and mediates fusion of the viral envelope with cellular membranes for infectious entry and spread.
33445487	1	22	gly	glycoprotein	104:115	arg1	glycoprotein					104:115	Envelope glycoprotein	95:115	Envelope glycoprotein (g)B	95:120	Envelope glycoprotein (g)B is conserved throughout the Herpesviridae and mediates fusion of the viral envelope with cellular membranes for infectious entry and spread.
33445487	2	23	link	-linked	336:342	arg1	glycosylation					344:356	asparagine (N)-linked glycosylation	322:356	asparagine (N)-linked glycosylation	322:356	Like all viral envelope fusion proteins, gB is modified by asparagine (N)-linked glycosylation.
33445487	5	24	from	sites	703:707	arg1	gB					716:717	PrV gB	712:717	PrV gB	712:717	Here, we investigated the occupancy and functional relevance of N-glycosylation sites in PrV gB.
33445487	7	25	theme	mutant	1007:1012	arg1	expression					910:919	expression	910:919	expression	910:919	The resulting proteins were tested for expression, fusion activity in cell-cell fusion assays and complementation of a gB-deficient PrV mutant.
33445487	7	25	theme	mutant	1007:1012	arg1	activity					929:936	fusion activity	922:936	fusion activity in cell-cell fusion assays	922:963	The resulting proteins were tested for expression, fusion activity in cell-cell fusion assays and complementation of a gB-deficient PrV mutant.
33445487	7	25	theme	mutant	1007:1012	arg1	complementation					969:983	complementation	969:983	complementation of a gB-deficient PrV mutant	969:1012	The resulting proteins were tested for expression, fusion activity in cell-cell fusion assays and complementation of a gB-deficient PrV mutant.
33445487	6	26	gly	N-glycosylation	747:761	arg2	sites					763:767	all predicted N-glycosylation sites	733:767	all predicted N-glycosylation sites	733:767	To this end, all predicted N-glycosylation sites were inactivated either singly or in combination by the introduction of conservative mutations (N➔Q).
33445487	10	27	theme	sites	1402:1406	arg1	inactivation					1362:1373	simultaneous inactivation	1349:1373	simultaneous inactivation of all six N-glycosylation sites	1349:1406	Although all single mutants were functional in cell-cell fusion and viral entry, simultaneous inactivation of all six N-glycosylation sites severely impaired fusion activity and viral entry, suggesting a critical role of N-glycans for maintaining gB structure and function.
33445487	10	28	from	fusion	1325:1330	arg1	functional					1301:1310	functional	1301:1310	functional	1301:1310	Although all single mutants were functional in cell-cell fusion and viral entry, simultaneous inactivation of all six N-glycosylation sites severely impaired fusion activity and viral entry, suggesting a critical role of N-glycans for maintaining gB structure and function.
33445487	10	29	theme	critical	1472:1479	arg1	role					1481:1484	a critical role	1470:1484	a critical role of N-glycans for maintaining gB structure and function	1470:1539	Although all single mutants were functional in cell-cell fusion and viral entry, simultaneous inactivation of all six N-glycosylation sites severely impaired fusion activity and viral entry, suggesting a critical role of N-glycans for maintaining gB structure and function.
33445487	10	30	theme	viral	1446:1450	arg1	entry					1452:1456	viral entry	1446:1456	viral entry	1446:1456	Although all single mutants were functional in cell-cell fusion and viral entry, simultaneous inactivation of all six N-glycosylation sites severely impaired fusion activity and viral entry, suggesting a critical role of N-glycans for maintaining gB structure and function.
33445487	6	31	theme	N-glycosylation	747:761	arg1	sites					763:767	all predicted N-glycosylation sites	733:767	all predicted N-glycosylation sites	733:767	To this end, all predicted N-glycosylation sites were inactivated either singly or in combination by the introduction of conservative mutations (N➔Q).
33445487	1	32	theme	Envelope	95:102	arg1	g					118:118	g	118:118	g	118:118	Envelope glycoprotein (g)B is conserved throughout the Herpesviridae and mediates fusion of the viral envelope with cellular membranes for infectious entry and spread.
33445487	1	32	theme	Envelope	95:102	arg1	glycoprotein					104:115	Envelope glycoprotein	95:115	Envelope glycoprotein (g)B	95:120	Envelope glycoprotein (g)B is conserved throughout the Herpesviridae and mediates fusion of the viral envelope with cellular membranes for infectious entry and spread.
33445487	6	33	dep	either	786:791	arg1	singly					793:798	singly	793:798	singly	793:798	To this end, all predicted N-glycosylation sites were inactivated either singly or in combination by the introduction of conservative mutations (N➔Q).
33445487	3	34	theme	intracellular	403:415	arg1	transport					417:425	intracellular transport	403:425	intracellular transport	403:425	Glycans can contribute to protein function, intracellular transport, trafficking, structure and immune evasion.
33445487	9	35	theme	N154	1187:1190	arg1	inactivation					1171:1182	inactivation	1171:1182	inactivation of N154 and N700	1171:1199	However, while glycosylation at most sites was dispensable for gB expression and fusogenicity, inactivation of N154 and N700 affected gB processing by furin cleavage and surface localization.
33445487	6	36	theme	predicted	737:745	arg1	sites					763:767	all predicted N-glycosylation sites	733:767	all predicted N-glycosylation sites	733:767	To this end, all predicted N-glycosylation sites were inactivated either singly or in combination by the introduction of conservative mutations (N➔Q).
33445487	4	37	contain	contains	523:530	arg2	sites					546:550	six consensus sites	532:550	six consensus sites for N-linked glycosylation	532:577	gB of the alphaherpesvirus pseudorabies virus (PrV) contains six consensus sites for N-linked glycosylation, but their functional relevance is unknown.
33445487	4	37	contain	contains	523:530	arg1	gB					471:472	gB	471:472	gB of the alphaherpesvirus pseudorabies virus (PrV)	471:521	gB of the alphaherpesvirus pseudorabies virus (PrV) contains six consensus sites for N-linked glycosylation, but their functional relevance is unknown.
33445487	1	38	dep	glycoprotein	104:115	arg1	B					120:120	B	120:120	Envelope glycoprotein (g)B	95:120	Envelope glycoprotein (g)B is conserved throughout the Herpesviridae and mediates fusion of the viral envelope with cellular membranes for infectious entry and spread.
33445487	10	39	theme	viral	1336:1340	arg1	entry					1342:1346	viral entry	1336:1346	viral entry	1336:1346	Although all single mutants were functional in cell-cell fusion and viral entry, simultaneous inactivation of all six N-glycosylation sites severely impaired fusion activity and viral entry, suggesting a critical role of N-glycans for maintaining gB structure and function.
33445487	0	40	theme	N-glycosylation	13:27	arg1	Influence					0:8	Influence	0:8	Influence of N-glycosylation on Expression and Function of Pseudorabies Virus Glycoprotein gB.	0:93	Influence of N-glycosylation on Expression and Function of Pseudorabies Virus Glycoprotein gB.
33445487	5	41	theme	N-glycosylation	687:701	arg1	sites					703:707	N-glycosylation sites	687:707	N-glycosylation sites in PrV gB	687:717	Here, we investigated the occupancy and functional relevance of N-glycosylation sites in PrV gB.
33445487	6	42	theme	mutations	854:862	arg1	introduction					825:836	the introduction	821:836	the introduction of conservative mutations (N➔Q)	821:868	To this end, all predicted N-glycosylation sites were inactivated either singly or in combination by the introduction of conservative mutations (N➔Q).
33445487	2	43	theme	fusion	287:292	arg1	proteins					294:301	all viral envelope fusion proteins	268:301	all viral envelope fusion proteins	268:301	Like all viral envelope fusion proteins, gB is modified by asparagine (N)-linked glycosylation.
33445487	10	44	theme	single	1281:1286	arg1	mutants					1288:1294	all single mutants	1277:1294	all single mutants	1277:1294	Although all single mutants were functional in cell-cell fusion and viral entry, simultaneous inactivation of all six N-glycosylation sites severely impaired fusion activity and viral entry, suggesting a critical role of N-glycans for maintaining gB structure and function.
33445487	1	45	theme	viral	191:195	arg1	envelope					197:204	the viral envelope	187:204	the viral envelope	187:204	Envelope glycoprotein (g)B is conserved throughout the Herpesviridae and mediates fusion of the viral envelope with cellular membranes for infectious entry and spread.
33445487	3	46	theme	protein	385:391	arg1	function					393:400	protein function	385:400	protein function	385:400	Glycans can contribute to protein function, intracellular transport, trafficking, structure and immune evasion.
33445487	4	47	theme	consensus	536:544	arg1	sites					546:550	six consensus sites	532:550	six consensus sites for N-linked glycosylation	532:577	gB of the alphaherpesvirus pseudorabies virus (PrV) contains six consensus sites for N-linked glycosylation, but their functional relevance is unknown.
33445487	5	48	theme	sites	703:707	arg1	occupancy					649:657	occupancy	649:657	occupancy	649:657	Here, we investigated the occupancy and functional relevance of N-glycosylation sites in PrV gB.
33445487	5	48	theme	sites	703:707	arg1	relevance					674:682	functional relevance	663:682	functional relevance	663:682	Here, we investigated the occupancy and functional relevance of N-glycosylation sites in PrV gB.
33445487	6	49	theme	conservative	841:852	arg1	N➔Q					865:867	N➔Q	865:867	N➔Q	865:867	To this end, all predicted N-glycosylation sites were inactivated either singly or in combination by the introduction of conservative mutations (N➔Q).
33445487	6	49	theme	conservative	841:852	arg1	mutations					854:862	conservative mutations	841:862	conservative mutations (N➔Q)	841:868	To this end, all predicted N-glycosylation sites were inactivated either singly or in combination by the introduction of conservative mutations (N➔Q).
33445487	7	50	from	complementation	969:983	arg1	assays					958:963	cell-cell fusion assays	941:963	cell-cell fusion assays	941:963	The resulting proteins were tested for expression, fusion activity in cell-cell fusion assays and complementation of a gB-deficient PrV mutant.
33445487	1	51	theme	envelope	197:204	arg1	fusion					177:182	fusion	177:182	fusion of the viral envelope with cellular membranes for infectious entry and spread	177:260	Envelope glycoprotein (g)B is conserved throughout the Herpesviridae and mediates fusion of the viral envelope with cellular membranes for infectious entry and spread.
33445487	9	52	theme	gB	1210:1211	arg1	processing					1213:1222	gB processing	1210:1222	gB processing	1210:1222	However, while glycosylation at most sites was dispensable for gB expression and fusogenicity, inactivation of N154 and N700 affected gB processing by furin cleavage and surface localization.
33445487	7	53	theme	gB-deficient	990:1001	arg1	mutant					1007:1012	a gB-deficient PrV mutant	988:1012	a gB-deficient PrV mutant	988:1012	The resulting proteins were tested for expression, fusion activity in cell-cell fusion assays and complementation of a gB-deficient PrV mutant.
33445487	10	54	from	entry	1342:1346	arg1	functional					1301:1310	functional	1301:1310	functional	1301:1310	Although all single mutants were functional in cell-cell fusion and viral entry, simultaneous inactivation of all six N-glycosylation sites severely impaired fusion activity and viral entry, suggesting a critical role of N-glycans for maintaining gB structure and function.
33445487	4	55	theme	virus	511:515	arg1	gB					471:472	gB	471:472	gB of the alphaherpesvirus pseudorabies virus (PrV)	471:521	gB of the alphaherpesvirus pseudorabies virus (PrV) contains six consensus sites for N-linked glycosylation, but their functional relevance is unknown.
33445487	1	56	with	fusion	177:182	arg1	membranes					220:228	cellular membranes	211:228	cellular membranes for infectious entry and spread	211:260	Envelope glycoprotein (g)B is conserved throughout the Herpesviridae and mediates fusion of the viral envelope with cellular membranes for infectious entry and spread.
33445487	5	57	from	gB	716:717	arg1	occupancy					649:657	occupancy	649:657	occupancy	649:657	Here, we investigated the occupancy and functional relevance of N-glycosylation sites in PrV gB.
33445487	5	57	from	gB	716:717	arg1	relevance					674:682	functional relevance	663:682	functional relevance	663:682	Here, we investigated the occupancy and functional relevance of N-glycosylation sites in PrV gB.
33445487	0	58	gly	N-glycosylation	13:27	arg1	gB					91:92	Pseudorabies Virus Glycoprotein gB	59:92	Pseudorabies Virus Glycoprotein gB	59:92	Influence of N-glycosylation on Expression and Function of Pseudorabies Virus Glycoprotein gB.
33445487	7	59	theme	PrV	1003:1005	arg1	mutant					1007:1012	a gB-deficient PrV mutant	988:1012	a gB-deficient PrV mutant	988:1012	The resulting proteins were tested for expression, fusion activity in cell-cell fusion assays and complementation of a gB-deficient PrV mutant.
33445487	5	60	gly	N-glycosylation	687:701	arg2	sites					703:707	N-glycosylation sites	687:707	N-glycosylation sites in PrV gB	687:717	Here, we investigated the occupancy and functional relevance of N-glycosylation sites in PrV gB.
33445487	4	61	theme	alphaherpesvirus	481:496	arg1	PrV					518:520	PrV	518:520	PrV	518:520	gB of the alphaherpesvirus pseudorabies virus (PrV) contains six consensus sites for N-linked glycosylation, but their functional relevance is unknown.
33445487	4	61	theme	alphaherpesvirus	481:496	arg1	virus					511:515	the alphaherpesvirus pseudorabies virus	477:515	the alphaherpesvirus pseudorabies virus (PrV)	477:521	gB of the alphaherpesvirus pseudorabies virus (PrV) contains six consensus sites for N-linked glycosylation, but their functional relevance is unknown.
33445487	10	62	theme	N-glycosylation	1386:1400	arg1	sites					1402:1406	all six N-glycosylation sites	1378:1406	all six N-glycosylation sites	1378:1406	Although all single mutants were functional in cell-cell fusion and viral entry, simultaneous inactivation of all six N-glycosylation sites severely impaired fusion activity and viral entry, suggesting a critical role of N-glycans for maintaining gB structure and function.
33445487	9	63	theme	most	1108:1111	arg1	sites					1113:1117	most sites	1108:1117	most sites	1108:1117	However, while glycosylation at most sites was dispensable for gB expression and fusogenicity, inactivation of N154 and N700 affected gB processing by furin cleavage and surface localization.
33445487	10	64	theme	simultaneous	1349:1360	arg1	inactivation					1362:1373	simultaneous inactivation	1349:1373	simultaneous inactivation of all six N-glycosylation sites	1349:1406	Although all single mutants were functional in cell-cell fusion and viral entry, simultaneous inactivation of all six N-glycosylation sites severely impaired fusion activity and viral entry, suggesting a critical role of N-glycans for maintaining gB structure and function.
33445487	5	65	theme	functional	663:672	arg1	relevance					674:682	functional relevance	663:682	functional relevance	663:682	Here, we investigated the occupancy and functional relevance of N-glycosylation sites in PrV gB.
33445487	9	66	theme	furin	1227:1231	arg1	cleavage					1233:1240	furin cleavage	1227:1240	furin cleavage	1227:1240	However, while glycosylation at most sites was dispensable for gB expression and fusogenicity, inactivation of N154 and N700 affected gB processing by furin cleavage and surface localization.
33445487	3	67	theme	immune	455:460	arg1	evasion					462:468	immune evasion	455:468	immune evasion	455:468	Glycans can contribute to protein function, intracellular transport, trafficking, structure and immune evasion.
33445487	2	68	mod	modified	310:317	arg3	glycosylation					344:356	asparagine (N)-linked glycosylation	322:356	asparagine (N)-linked glycosylation	322:356	Like all viral envelope fusion proteins, gB is modified by asparagine (N)-linked glycosylation.
33445487	2	68	mod	modified	310:317	arg1	gB					304:305	gB	304:305	gB	304:305	Like all viral envelope fusion proteins, gB is modified by asparagine (N)-linked glycosylation.
33445487	1	69	theme	cellular	211:218	arg1	membranes					220:228	cellular membranes	211:228	cellular membranes for infectious entry and spread	211:260	Envelope glycoprotein (g)B is conserved throughout the Herpesviridae and mediates fusion of the viral envelope with cellular membranes for infectious entry and spread.
33445487	9	70	gly	glycosylation	1091:1103	arg1	sites					1113:1117	most sites	1108:1117	most sites	1108:1117	However, while glycosylation at most sites was dispensable for gB expression and fusogenicity, inactivation of N154 and N700 affected gB processing by furin cleavage and surface localization.
33445487	9	70	gly	glycosylation	1091:1103	arg2	sites					1113:1117	most sites	1108:1117	most sites	1108:1117	However, while glycosylation at most sites was dispensable for gB expression and fusogenicity, inactivation of N154 and N700 affected gB processing by furin cleavage and surface localization.
33445487	4	71	link	N-linked	556:563	arg1	glycosylation					565:577	N-linked glycosylation	556:577	N-linked glycosylation	556:577	gB of the alphaherpesvirus pseudorabies virus (PrV) contains six consensus sites for N-linked glycosylation, but their functional relevance is unknown.
33445487	6	72	dep	inactivated	774:784	arg1	either					786:791	either	786:791	either	786:791	To this end, all predicted N-glycosylation sites were inactivated either singly or in combination by the introduction of conservative mutations (N➔Q).
33445487	6	72	dep	inactivated	774:784	arg1	combination					806:816	combination	806:816	combination	806:816	To this end, all predicted N-glycosylation sites were inactivated either singly or in combination by the introduction of conservative mutations (N➔Q).
33445487	5	73	from	relevance	674:682	arg1	gB					716:717	PrV gB	712:717	PrV gB	712:717	Here, we investigated the occupancy and functional relevance of N-glycosylation sites in PrV gB.
33445487	0	74	theme	Virus	72:76	arg1	gB					91:92	Pseudorabies Virus Glycoprotein gB	59:92	Pseudorabies Virus Glycoprotein gB	59:92	Influence of N-glycosylation on Expression and Function of Pseudorabies Virus Glycoprotein gB.
33445487	2	75	theme	-linked	336:342	arg1	glycosylation					344:356	asparagine (N)-linked glycosylation	322:356	asparagine (N)-linked glycosylation	322:356	Like all viral envelope fusion proteins, gB is modified by asparagine (N)-linked glycosylation.
33445487	7	76	theme	resulting	875:883	arg1	proteins					885:892	The resulting proteins	871:892	The resulting proteins	871:892	The resulting proteins were tested for expression, fusion activity in cell-cell fusion assays and complementation of a gB-deficient PrV mutant.
33445487	5	77	from	occupancy	649:657	arg1	gB					716:717	PrV gB	712:717	PrV gB	712:717	Here, we investigated the occupancy and functional relevance of N-glycosylation sites in PrV gB.
33445487	0	78	theme	Pseudorabies	59:70	arg1	gB					91:92	Pseudorabies Virus Glycoprotein gB	59:92	Pseudorabies Virus Glycoprotein gB	59:92	Influence of N-glycosylation on Expression and Function of Pseudorabies Virus Glycoprotein gB.
33445487	9	79	theme	surface	1246:1252	arg1	localization					1254:1265	surface localization	1246:1265	surface localization	1246:1265	However, while glycosylation at most sites was dispensable for gB expression and fusogenicity, inactivation of N154 and N700 affected gB processing by furin cleavage and surface localization.
33445487	4	80	theme	functional	590:599	arg1	relevance					601:609	their functional relevance	584:609	their functional relevance	584:609	gB of the alphaherpesvirus pseudorabies virus (PrV) contains six consensus sites for N-linked glycosylation, but their functional relevance is unknown.
33445487	7	81	theme	fusion	951:956	arg1	assays					958:963	cell-cell fusion assays	941:963	cell-cell fusion assays	941:963	The resulting proteins were tested for expression, fusion activity in cell-cell fusion assays and complementation of a gB-deficient PrV mutant.
33445487	5	82	gly	occupancy	649:657	arg2	sites					703:707	N-glycosylation sites	687:707	N-glycosylation sites in PrV gB	687:717	Here, we investigated the occupancy and functional relevance of N-glycosylation sites in PrV gB.
33445487	1	83	theme	infectious	234:243	arg1	entry					245:249	infectious entry	234:249	infectious entry	234:249	Envelope glycoprotein (g)B is conserved throughout the Herpesviridae and mediates fusion of the viral envelope with cellular membranes for infectious entry and spread.
32971949	8	0	theme	antioxidant	1068:1078	arg1	system					1088:1093	the intestinal cellular antioxidant defense system	1044:1093	the intestinal cellular antioxidant defense system	1044:1093	The results indicated that both fractions displayed antioxidant properties through mediation of the intestinal cellular antioxidant defense system, which could protect cultured intestinal cells from oxidative stress-induced damage and cell viability suppression.
32971949	8	1	theme	stress-induced	1157:1170	arg1	damage					1172:1177	oxidative stress-induced damage	1147:1177	oxidative stress-induced damage	1147:1177	The results indicated that both fractions displayed antioxidant properties through mediation of the intestinal cellular antioxidant defense system, which could protect cultured intestinal cells from oxidative stress-induced damage and cell viability suppression.
32971949	8	2	theme	cellular	1059:1066	arg1	system					1088:1093	the intestinal cellular antioxidant defense system	1044:1093	the intestinal cellular antioxidant defense system	1044:1093	The results indicated that both fractions displayed antioxidant properties through mediation of the intestinal cellular antioxidant defense system, which could protect cultured intestinal cells from oxidative stress-induced damage and cell viability suppression.
32971949	8	3	theme	antioxidant	1000:1010	arg1	properties					1012:1021	antioxidant properties	1000:1021	antioxidant properties	1000:1021	The results indicated that both fractions displayed antioxidant properties through mediation of the intestinal cellular antioxidant defense system, which could protect cultured intestinal cells from oxidative stress-induced damage and cell viability suppression.
32971949	9	4	from	difference	1291:1300	arg1	structure					1305:1313	structure	1305:1313	structure	1305:1313	The TPD-I fraction showed stronger antioxidant effects, which may be due to the difference in structure.
32971949	8	5	theme	system	1088:1093	arg1	mediation					1031:1039	mediation	1031:1039	mediation of the intestinal cellular antioxidant defense system, which could protect cultured intestinal cells from oxidative stress-induced damage and cell viability suppression	1031:1208	The results indicated that both fractions displayed antioxidant properties through mediation of the intestinal cellular antioxidant defense system, which could protect cultured intestinal cells from oxidative stress-induced damage and cell viability suppression.
32971949	9	6	theme	antioxidant	1246:1256	arg1	effects					1258:1264	stronger antioxidant effects	1237:1264	stronger antioxidant effects	1237:1264	The TPD-I fraction showed stronger antioxidant effects, which may be due to the difference in structure.
32971949	3	7	theme	polysaccharide	354:367	arg1	purification					369:380	polysaccharide purification	354:380	polysaccharide purification from two different species of Chinese truffles	354:427	In this study, Diethylaminoethyl anion exchange chromatography and gel filtration were employed for polysaccharide purification from two different species of Chinese truffles.
32971949	0	8	from	Activity	52:59	arg1	Species					89:95	Two Species	85:95	Two Species of Chinese Truffles	85:115	Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles.
32971949	8	9	theme	defense	1080:1086	arg1	system					1088:1093	the intestinal cellular antioxidant defense system	1044:1093	the intestinal cellular antioxidant defense system	1044:1093	The results indicated that both fractions displayed antioxidant properties through mediation of the intestinal cellular antioxidant defense system, which could protect cultured intestinal cells from oxidative stress-induced damage and cell viability suppression.
32971949	0	10	theme	Truffles	108:115	arg1	Species					89:95	Two Species	85:95	Two Species of Chinese Truffles	85:115	Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles.
32971949	10	11	theme	Further	1316:1322	arg1	study					1324:1328	Further study	1316:1328	Further study on the structure-activity relationship	1316:1367	Further study on the structure-activity relationship is needed to be done.
32971949	6	12	from	species	735:741	arg1	polysaccharide					705:718	the polysaccharide	701:718	the polysaccharide from different species	701:741	The results of structural elucidation indicated that the polysaccharide from different species showed different monosaccharide composition and linkage units, as well as molecular weight.
32971949	6	13	theme	different	725:733	arg1	species					735:741	different species	725:741	different species	725:741	The results of structural elucidation indicated that the polysaccharide from different species showed different monosaccharide composition and linkage units, as well as molecular weight.
32971949	0	14	theme	Chinese	100:106	arg1	Truffles					108:115	Chinese Truffles	100:115	Chinese Truffles	100:115	Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles.
32971949	6	15	theme	molecular	817:825	arg1	weight					827:832	molecular weight	817:832	molecular weight	817:832	The results of structural elucidation indicated that the polysaccharide from different species showed different monosaccharide composition and linkage units, as well as molecular weight.
32971949	8	16	dep	damage	1172:1177	arg1	suppression					1198:1208	suppression	1198:1208	suppression	1198:1208	The results indicated that both fractions displayed antioxidant properties through mediation of the intestinal cellular antioxidant defense system, which could protect cultured intestinal cells from oxidative stress-induced damage and cell viability suppression.
32971949	0	17	from	Structure	26:34	arg1	Species					89:95	Two Species	85:95	Two Species of Chinese Truffles	85:115	Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles.
32971949	0	18	theme	Polysaccharides	64:78	arg1	Structure					26:34	Partial Structure	18:34	Partial Structure	18:34	Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles.
32971949	0	18	theme	Polysaccharides	64:78	arg1	Activity					52:59	Antioxidant Activity	40:59	Antioxidant Activity	40:59	Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles.
32971949	7	19	theme	highest	880:886	arg1	yield					888:892	the highest yield	876:892	the highest yield	876:892	Two of the polysaccharide fractions with the highest yield, TPZ-I and TPD-I, were chosen for biological testing.
32971949	3	20	theme	different	391:399	arg1	species					401:407	two different species	387:407	two different species of Chinese truffles	387:427	In this study, Diethylaminoethyl anion exchange chromatography and gel filtration were employed for polysaccharide purification from two different species of Chinese truffles.
32971949	8	21	theme	oxidative	1147:1155	arg1	damage					1172:1177	oxidative stress-induced damage	1147:1177	oxidative stress-induced damage	1147:1177	The results indicated that both fractions displayed antioxidant properties through mediation of the intestinal cellular antioxidant defense system, which could protect cultured intestinal cells from oxidative stress-induced damage and cell viability suppression.
32971949	4	22	theme	polysaccharide	436:449	arg1	TPZ-II					539:544	TPZ-II	539:544	TPZ-II	539:544	Three polysaccharide fractions were obtained from Tuber panzhihuanense and referred to as TPZ-NP, TPZ-I, and TPZ-II.
32971949	4	22	theme	polysaccharide	436:449	arg1	TPZ-NP					520:525	TPZ-NP	520:525	TPZ-NP	520:525	Three polysaccharide fractions were obtained from Tuber panzhihuanense and referred to as TPZ-NP, TPZ-I, and TPZ-II.
32971949	4	22	theme	polysaccharide	436:449	arg1	fractions					451:459	Three polysaccharide fractions	430:459	Three polysaccharide fractions	430:459	Three polysaccharide fractions were obtained from Tuber panzhihuanense and referred to as TPZ-NP, TPZ-I, and TPZ-II.
32971949	4	22	theme	polysaccharide	436:449	arg1	TPZ-I					528:532	TPZ-I	528:532	TPZ-I	528:532	Three polysaccharide fractions were obtained from Tuber panzhihuanense and referred to as TPZ-NP, TPZ-I, and TPZ-II.
32971949	8	23	theme	intestinal	1048:1057	arg1	system					1088:1093	the intestinal cellular antioxidant defense system	1044:1093	the intestinal cellular antioxidant defense system	1044:1093	The results indicated that both fractions displayed antioxidant properties through mediation of the intestinal cellular antioxidant defense system, which could protect cultured intestinal cells from oxidative stress-induced damage and cell viability suppression.
32971949	7	24	theme	fractions	861:869	arg1	fractions					861:869	the polysaccharide fractions	842:869	the polysaccharide fractions	842:869	Two of the polysaccharide fractions with the highest yield, TPZ-I and TPD-I, were chosen for biological testing.
32971949	7	24	theme	fractions	861:869	arg1	TPD-I					905:909	TPD-I	905:909	TPD-I	905:909	Two of the polysaccharide fractions with the highest yield, TPZ-I and TPD-I, were chosen for biological testing.
32971949	7	24	theme	fractions	861:869	arg1	TPZ-I					895:899	TPZ-I	895:899	TPZ-I	895:899	Two of the polysaccharide fractions with the highest yield, TPZ-I and TPD-I, were chosen for biological testing.
32971949	7	24	theme	fractions	861:869	arg1	Two					835:837	Two	835:837	Two	835:837	Two of the polysaccharide fractions with the highest yield, TPZ-I and TPD-I, were chosen for biological testing.
32971949	0	25	theme	Partial	18:24	arg1	Structure					26:34	Partial Structure	18:34	Partial Structure	18:34	Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles.
32971949	8	26	theme	intestinal	1125:1134	arg1	cells					1136:1140	cultured intestinal cells	1116:1140	cultured intestinal cells	1116:1140	The results indicated that both fractions displayed antioxidant properties through mediation of the intestinal cellular antioxidant defense system, which could protect cultured intestinal cells from oxidative stress-induced damage and cell viability suppression.
32971949	10	27	from	study	1324:1328	arg1	relationship					1356:1367	the structure-activity relationship	1333:1367	the structure-activity relationship	1333:1367	Further study on the structure-activity relationship is needed to be done.
32971949	9	28	theme	stronger	1237:1244	arg1	effects					1258:1264	stronger antioxidant effects	1237:1264	stronger antioxidant effects	1237:1264	The TPD-I fraction showed stronger antioxidant effects, which may be due to the difference in structure.
32971949	9	29	theme	TPD-I	1215:1219	arg1	fraction					1221:1228	The TPD-I fraction	1211:1228	The TPD-I fraction	1211:1228	The TPD-I fraction showed stronger antioxidant effects, which may be due to the difference in structure.
32971949	8	30	theme	cultured	1116:1123	arg1	cells					1136:1140	cultured intestinal cells	1116:1140	cultured intestinal cells	1116:1140	The results indicated that both fractions displayed antioxidant properties through mediation of the intestinal cellular antioxidant defense system, which could protect cultured intestinal cells from oxidative stress-induced damage and cell viability suppression.
32971949	7	31	theme	polysaccharide	846:859	arg1	fractions					861:869	the polysaccharide fractions	842:869	the polysaccharide fractions	842:869	Two of the polysaccharide fractions with the highest yield, TPZ-I and TPD-I, were chosen for biological testing.
32971949	3	32	theme	Chinese	412:418	arg1	truffles					420:427	Chinese truffles	412:427	Chinese truffles	412:427	In this study, Diethylaminoethyl anion exchange chromatography and gel filtration were employed for polysaccharide purification from two different species of Chinese truffles.
32971949	10	33	theme	structure-activity	1337:1354	arg1	relationship					1356:1367	the structure-activity relationship	1333:1367	the structure-activity relationship	1333:1367	Further study on the structure-activity relationship is needed to be done.
32971949	2	34	theme	unique	180:185	arg1	aroma					187:191	their unique aroma	174:191	their unique aroma	174:191	Due to their unique aroma and rarity, the price of truffles has always been very high.
32971949	6	35	theme	elucidation	674:684	arg1	results					652:658	The results	648:658	The results of structural elucidation	648:684	The results of structural elucidation indicated that the polysaccharide from different species showed different monosaccharide composition and linkage units, as well as molecular weight.
32971949	0	36	theme	Structure	26:34	arg1	Comparison					0:9	Comparison	0:9	Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles.	0:116	Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles.
32971949	3	37	theme	truffles	420:427	arg1	species					401:407	two different species	387:407	two different species of Chinese truffles	387:427	In this study, Diethylaminoethyl anion exchange chromatography and gel filtration were employed for polysaccharide purification from two different species of Chinese truffles.
32971949	4	38	theme	Tuber	480:484	arg1	panzhihuanense					486:499	Tuber panzhihuanense	480:499	Tuber panzhihuanense	480:499	Three polysaccharide fractions were obtained from Tuber panzhihuanense and referred to as TPZ-NP, TPZ-I, and TPZ-II.
32971949	2	39	theme	truffles	218:225	arg1	price					209:213	the price	205:213	the price of truffles	205:225	Due to their unique aroma and rarity, the price of truffles has always been very high.
32971949	2	39	theme	truffles	218:225	arg1	high					248:251	high	248:251	high	248:251	Due to their unique aroma and rarity, the price of truffles has always been very high.
32971949	0	40	theme	Activity	52:59	arg1	Comparison					0:9	Comparison	0:9	Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles.	0:116	Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles.
32971949	3	41	theme	anion	287:291	arg1	chromatography					302:315	Diethylaminoethyl anion exchange chromatography	269:315	Diethylaminoethyl anion exchange chromatography	269:315	In this study, Diethylaminoethyl anion exchange chromatography and gel filtration were employed for polysaccharide purification from two different species of Chinese truffles.
32971949	0	42	theme	Antioxidant	40:50	arg1	Activity					52:59	Antioxidant Activity	40:59	Antioxidant Activity	40:59	Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles.
32971949	3	43	theme	exchange	293:300	arg1	chromatography					302:315	Diethylaminoethyl anion exchange chromatography	269:315	Diethylaminoethyl anion exchange chromatography	269:315	In this study, Diethylaminoethyl anion exchange chromatography and gel filtration were employed for polysaccharide purification from two different species of Chinese truffles.
32971949	0	44	dep	Structure	26:34	arg1	the					14:16	the	14:16	the	14:16	Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles.
32971949	6	45	theme	linkage	791:797	arg1	units					799:803	linkage units	791:803	linkage units	791:803	The results of structural elucidation indicated that the polysaccharide from different species showed different monosaccharide composition and linkage units, as well as molecular weight.
32971949	6	46	theme	structural	663:672	arg1	elucidation					674:684	structural elucidation	663:684	structural elucidation	663:684	The results of structural elucidation indicated that the polysaccharide from different species showed different monosaccharide composition and linkage units, as well as molecular weight.
32971949	5	47	theme	polysaccharide	565:578	arg1	fractions					580:588	two polysaccharide fractions	561:588	two polysaccharide fractions	561:588	Additionally, two polysaccharide fractions were purified from T. pseudoexcavatum (TPD-NP and TPD-I).
32971949	7	48	theme	biological	928:937	arg1	testing					939:945	biological testing	928:945	biological testing	928:945	Two of the polysaccharide fractions with the highest yield, TPZ-I and TPD-I, were chosen for biological testing.
32971949	3	49	theme	Diethylaminoethyl	269:285	arg1	chromatography					302:315	Diethylaminoethyl anion exchange chromatography	269:315	Diethylaminoethyl anion exchange chromatography	269:315	In this study, Diethylaminoethyl anion exchange chromatography and gel filtration were employed for polysaccharide purification from two different species of Chinese truffles.
32971949	1	50	theme	world-renowned	131:144	arg1	commodities					154:164	world-renowned premium commodities	131:164	world-renowned premium commodities	131:164	Truffles are world-renowned premium commodities.
32971949	1	50	theme	world-renowned	131:144	arg1	Truffles					118:125	Truffles	118:125	Truffles	118:125	Truffles are world-renowned premium commodities.
32971949	3	51	theme	gel	321:323	arg1	filtration					325:334	gel filtration	321:334	gel filtration	321:334	In this study, Diethylaminoethyl anion exchange chromatography and gel filtration were employed for polysaccharide purification from two different species of Chinese truffles.
32971949	0	52	from	Species	89:95	arg1	Polysaccharides					64:78	Polysaccharides	64:78	Polysaccharides from Two Species of Chinese Truffles	64:115	Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles.
32971949	0	52	from	Species	89:95	arg1	Structure					26:34	Partial Structure	18:34	Partial Structure	18:34	Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles.
32971949	0	52	from	Species	89:95	arg1	Activity					52:59	Antioxidant Activity	40:59	Antioxidant Activity	40:59	Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles.
32971949	6	53	theme	monosaccharide	760:773	arg1	composition					775:785	different monosaccharide composition	750:785	different monosaccharide composition	750:785	The results of structural elucidation indicated that the polysaccharide from different species showed different monosaccharide composition and linkage units, as well as molecular weight.
32971949	1	54	theme	premium	146:152	arg1	commodities					154:164	world-renowned premium commodities	131:164	world-renowned premium commodities	131:164	Truffles are world-renowned premium commodities.
32971949	1	54	theme	premium	146:152	arg1	Truffles					118:125	Truffles	118:125	Truffles	118:125	Truffles are world-renowned premium commodities.
32971949	5	55	dep	pseudoexcavatum	612:626	arg1	TPD-I					640:644	TPD-I	640:644	TPD-I	640:644	Additionally, two polysaccharide fractions were purified from T. pseudoexcavatum (TPD-NP and TPD-I).
32971949	5	55	dep	pseudoexcavatum	612:626	arg1	TPD-NP					629:634	TPD-NP	629:634	TPD-NP	629:634	Additionally, two polysaccharide fractions were purified from T. pseudoexcavatum (TPD-NP and TPD-I).
32971949	8	56	theme	cell	1183:1186	arg1	viability					1188:1196	cell viability	1183:1196	cell viability	1183:1196	The results indicated that both fractions displayed antioxidant properties through mediation of the intestinal cellular antioxidant defense system, which could protect cultured intestinal cells from oxidative stress-induced damage and cell viability suppression.
32971949	3	57	from	species	401:407	arg1	purification					369:380	polysaccharide purification	354:380	polysaccharide purification from two different species of Chinese truffles	354:427	In this study, Diethylaminoethyl anion exchange chromatography and gel filtration were employed for polysaccharide purification from two different species of Chinese truffles.
32971949	6	58	theme	different	750:758	arg1	composition					775:785	different monosaccharide composition	750:785	different monosaccharide composition	750:785	The results of structural elucidation indicated that the polysaccharide from different species showed different monosaccharide composition and linkage units, as well as molecular weight.
34225075	6	0	theme	X-ray	1219:1223	arg1	GIXRD					1238:1242	GIXRD	1238:1242	GIXRD	1238:1242	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	6	0	theme	X-ray	1219:1223	arg1	diffraction					1225:1235	Grazing incidence X-ray diffraction	1201:1235	Grazing incidence X-ray diffraction (GIXRD) experiments	1201:1255	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	10	1	theme	dispersion-corrected	1770:1789	arg1	explorations					1825:1836	The dispersion-corrected Density Functional Theory (DFT)-D explorations	1766:1836	The dispersion-corrected Density Functional Theory (DFT)-D explorations ascertained the inclusion capacity of benzimidazole	1766:1888	The dispersion-corrected Density Functional Theory (DFT)-D explorations ascertained the inclusion capacity of benzimidazole inside the β-CD.
34225075	6	2	theme	Grazing	1201:1207	arg1	GIXRD					1238:1242	GIXRD	1238:1242	GIXRD	1238:1242	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	6	2	theme	Grazing	1201:1207	arg1	diffraction					1225:1235	Grazing incidence X-ray diffraction	1201:1235	Grazing incidence X-ray diffraction (GIXRD) experiments	1201:1255	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	4	3	theme	macromolecule	697:709	arg1	structure					672:680	The chemical structure	659:680	EXPERIMENTS The chemical structure of the β-CD-BM macromolecule	647:709	EXPERIMENTS The chemical structure of the β-CD-BM macromolecule was explored by Fourier-transform infrared spectroscopy (FT-IR), X-Ray diffraction (XRD), and Ultraviolet-visible spectroscopy (UV-Vis).
34225075	1	4	theme	eco-friendly	188:199	arg1	system					201:206	an eco-friendly system	185:206	an eco-friendly system for reducing the metals' corrosion in NaCl solutions	185:259	HYPOTHESIS Silane (sol-gel)-based coatings have been introduced as an eco-friendly system for reducing the metals' corrosion in NaCl solutions.
34225075	1	4	theme	eco-friendly	188:199	arg1	coatings					152:159	HYPOTHESIS Silane (sol-gel)-based coatings	118:159	HYPOTHESIS Silane (sol-gel)-based coatings	118:159	HYPOTHESIS Silane (sol-gel)-based coatings have been introduced as an eco-friendly system for reducing the metals' corrosion in NaCl solutions.
34225075	2	5	theme	property	308:315	arg1	lack					282:285	the lack	278:285	the lack of active protection property for this type of coatings	278:341	However, due to the lack of active protection property for this type of coatings, their modification is totally recommended for achieving durable protection properties.
34225075	4	6	dep	spectroscopy	825:836	arg1	UV-Vis					839:844	UV-Vis	839:844	UV-Vis	839:844	EXPERIMENTS The chemical structure of the β-CD-BM macromolecule was explored by Fourier-transform infrared spectroscopy (FT-IR), X-Ray diffraction (XRD), and Ultraviolet-visible spectroscopy (UV-Vis).
34225075	9	7	theme	intact	1707:1712	arg1	coating					1714:1720	the intact coating	1703:1720	the intact coating	1703:1720	The EIS results also displayed that the total resistance of the modified composite was enhanced from 5540 Ω.cm2 to 10967 Ω.cm2 and the intact coating provided a total resistance of 80254 Ω.cm2.
34225075	3	8	theme	reliable	576:583	arg1	property					603:610	reliable active protection property	576:610	reliable active protection property	576:610	The present study introduces Beta-cyclodextrin (β-CD) as a novel/effective organic nano-container for Benzimidazole (BM) encapsulation to obtain reliable active protection property via a controlled-release property.
34225075	5	9	theme	Impedance	877:885	arg1	Spectroscopy					887:898	the Electrochemical Impedance Spectroscopy	857:898	the Electrochemical Impedance Spectroscopy (EIS)	857:904	Besides, the Electrochemical Impedance Spectroscopy (EIS) and polarization (potentiodynamic) tests were carried out for investigating the inhibition impacts of the constructed containers.
34225075	5	9	theme	Impedance	877:885	arg1	EIS					901:903	EIS	901:903	EIS	901:903	Besides, the Electrochemical Impedance Spectroscopy (EIS) and polarization (potentiodynamic) tests were carried out for investigating the inhibition impacts of the constructed containers.
34225075	3	10	theme	controlled-release	618:635	arg1	property					637:644	a controlled-release property	616:644	a controlled-release property	616:644	The present study introduces Beta-cyclodextrin (β-CD) as a novel/effective organic nano-container for Benzimidazole (BM) encapsulation to obtain reliable active protection property via a controlled-release property.
34225075	9	11	theme	total	1733:1737	arg1	resistance					1739:1748	a total resistance	1731:1748	a total resistance of 80254 Ω.cm2	1731:1763	The EIS results also displayed that the total resistance of the modified composite was enhanced from 5540 Ω.cm2 to 10967 Ω.cm2 and the intact coating provided a total resistance of 80254 Ω.cm2.
34225075	8	12	theme	complexes	1485:1493	arg1	addition					1465:1472	the addition	1461:1472	the addition of β-CD-BM complexes to the saline solution	1461:1516	FINDINGS The EIS achievements demonstrated that by the addition of β-CD-BM complexes to the saline solution, the mild steel corrosion was mitigated by about 84%.
34225075	7	13	theme	constructed	1386:1396	arg1	composites					1398:1407	the constructed composites	1382:1407	the constructed composites	1382:1407	Also, the EIS test was conducted over the Silane-based composite film (SCF) for analyzing the anti-corrosion performance of the constructed composites.
34225075	1	14	theme	Silane	129:134	arg1	system					201:206	an eco-friendly system	185:206	an eco-friendly system for reducing the metals' corrosion in NaCl solutions	185:259	HYPOTHESIS Silane (sol-gel)-based coatings have been introduced as an eco-friendly system for reducing the metals' corrosion in NaCl solutions.
34225075	1	14	theme	Silane	129:134	arg1	coatings					152:159	HYPOTHESIS Silane (sol-gel)-based coatings	118:159	HYPOTHESIS Silane (sol-gel)-based coatings	118:159	HYPOTHESIS Silane (sol-gel)-based coatings have been introduced as an eco-friendly system for reducing the metals' corrosion in NaCl solutions.
34225075	7	15	theme	anti-corrosion	1352:1365	arg1	performance					1367:1377	the anti-corrosion performance	1348:1377	the anti-corrosion performance of the constructed composites	1348:1407	Also, the EIS test was conducted over the Silane-based composite film (SCF) for analyzing the anti-corrosion performance of the constructed composites.
34225075	4	16	theme	Fourier-transform	727:743	arg1	FT-IR					768:772	FT-IR	768:772	FT-IR	768:772	EXPERIMENTS The chemical structure of the β-CD-BM macromolecule was explored by Fourier-transform infrared spectroscopy (FT-IR), X-Ray diffraction (XRD), and Ultraviolet-visible spectroscopy (UV-Vis).
34225075	4	16	theme	Fourier-transform	727:743	arg1	spectroscopy					754:765	Fourier-transform infrared spectroscopy	727:765	Fourier-transform infrared spectroscopy (FT-IR)	727:773	EXPERIMENTS The chemical structure of the β-CD-BM macromolecule was explored by Fourier-transform infrared spectroscopy (FT-IR), X-Ray diffraction (XRD), and Ultraviolet-visible spectroscopy (UV-Vis).
34225075	6	17	theme	unexposed	1052:1060	arg1	samples					1062:1068	The exposed and unexposed samples	1036:1068	The exposed and unexposed samples	1036:1068	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	0	18	theme	experimental/computational	78:103	arg1	assessments					105:115	experimental/computational assessments	78:115	experimental/computational assessments	78:115	Benzimidazole loaded β-cyclodextrin as a novel anti-corrosion system; coupled experimental/computational assessments.
34225075	6	19	theme	exposed	1040:1046	arg1	samples					1062:1068	The exposed and unexposed samples	1036:1068	The exposed and unexposed samples	1036:1068	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	8	20	theme	steel	1528:1532	arg1	corrosion					1534:1542	the mild steel corrosion	1519:1542	the mild steel corrosion	1519:1542	FINDINGS The EIS achievements demonstrated that by the addition of β-CD-BM complexes to the saline solution, the mild steel corrosion was mitigated by about 84%.
34225075	4	21	theme	Ultraviolet-visible	805:823	arg1	spectroscopy					825:836	Ultraviolet-visible spectroscopy	805:836	Ultraviolet-visible spectroscopy (UV-Vis)	805:845	EXPERIMENTS The chemical structure of the β-CD-BM macromolecule was explored by Fourier-transform infrared spectroscopy (FT-IR), X-Ray diffraction (XRD), and Ultraviolet-visible spectroscopy (UV-Vis).
34225075	11	22	theme	MC/MD	1943:1947	arg1	calculations					1950:1961	The Monte Carlo/Molecular Dynamics (MC/MD) calculations	1907:1961	The Monte Carlo/Molecular Dynamics (MC/MD) calculations	1907:1961	The Monte Carlo/Molecular Dynamics (MC/MD) calculations strongly affirmed the adsorption of BM and β-CD-BM over the substrate.
34225075	5	23	theme	containers	1024:1033	arg1	impacts					997:1003	the inhibition impacts	982:1003	the inhibition impacts of the constructed containers	982:1033	Besides, the Electrochemical Impedance Spectroscopy (EIS) and polarization (potentiodynamic) tests were carried out for investigating the inhibition impacts of the constructed containers.
34225075	6	24	theme	Electron	1121:1128	arg1	FE-SEM					1142:1147	FE-SEM	1142:1147	FE-SEM	1142:1147	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	6	24	theme	Electron	1121:1128	arg1	Microscope					1130:1139	Field Emission Scanning Electron Microscope	1097:1139	Field Emission Scanning Electron Microscope (FE-SEM)	1097:1148	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	11	25	theme	Dynamics	1933:1940	arg1	calculations					1950:1961	The Monte Carlo/Molecular Dynamics (MC/MD) calculations	1907:1961	The Monte Carlo/Molecular Dynamics (MC/MD) calculations	1907:1961	The Monte Carlo/Molecular Dynamics (MC/MD) calculations strongly affirmed the adsorption of BM and β-CD-BM over the substrate.
34225075	1	26	theme	HYPOTHESIS	118:127	arg1	system					201:206	an eco-friendly system	185:206	an eco-friendly system for reducing the metals' corrosion in NaCl solutions	185:259	HYPOTHESIS Silane (sol-gel)-based coatings have been introduced as an eco-friendly system for reducing the metals' corrosion in NaCl solutions.
34225075	1	26	theme	HYPOTHESIS	118:127	arg1	coatings					152:159	HYPOTHESIS Silane (sol-gel)-based coatings	118:159	HYPOTHESIS Silane (sol-gel)-based coatings	118:159	HYPOTHESIS Silane (sol-gel)-based coatings have been introduced as an eco-friendly system for reducing the metals' corrosion in NaCl solutions.
34225075	6	27	theme	Emission	1103:1110	arg1	FE-SEM					1142:1147	FE-SEM	1142:1147	FE-SEM	1142:1147	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	6	27	theme	Emission	1103:1110	arg1	Microscope					1130:1139	Field Emission Scanning Electron Microscope	1097:1139	Field Emission Scanning Electron Microscope (FE-SEM)	1097:1148	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	10	28	theme	inclusion	1854:1862	arg1	capacity					1864:1871	the inclusion capacity	1850:1871	the inclusion capacity of benzimidazole	1850:1888	The dispersion-corrected Density Functional Theory (DFT)-D explorations ascertained the inclusion capacity of benzimidazole inside the β-CD.
34225075	8	29	theme	EIS	1423:1425	arg1	achievements					1427:1438	The EIS achievements	1419:1438	FINDINGS The EIS achievements	1410:1438	FINDINGS The EIS achievements demonstrated that by the addition of β-CD-BM complexes to the saline solution, the mild steel corrosion was mitigated by about 84%.
34225075	2	30	theme	protection	408:417	arg1	properties					419:428	durable protection properties	400:428	durable protection properties	400:428	However, due to the lack of active protection property for this type of coatings, their modification is totally recommended for achieving durable protection properties.
34225075	10	31	theme	benzimidazole	1876:1888	arg1	capacity					1864:1871	the inclusion capacity	1850:1871	the inclusion capacity of benzimidazole	1850:1888	The dispersion-corrected Density Functional Theory (DFT)-D explorations ascertained the inclusion capacity of benzimidazole inside the β-CD.
34225075	2	32	theme	coatings	334:341	arg1	type					326:329	this type	321:329	this type of coatings	321:341	However, due to the lack of active protection property for this type of coatings, their modification is totally recommended for achieving durable protection properties.
34225075	0	33	theme	anti-corrosion	47:60	arg1	β-cyclodextrin					21:34	β-cyclodextrin	21:34	β-cyclodextrin	21:34	Benzimidazole loaded β-cyclodextrin as a novel anti-corrosion system; coupled experimental/computational assessments.
34225075	0	33	theme	anti-corrosion	47:60	arg1	system					62:67	a novel anti-corrosion system	39:67	a novel anti-corrosion system	39:67	Benzimidazole loaded β-cyclodextrin as a novel anti-corrosion system; coupled experimental/computational assessments.
34225075	6	34	theme	Spectroscopy	1169:1180	arg1	/mapping					1187:1194	Energy Dispersive Spectroscopy (EDS)/mapping	1151:1194	Energy Dispersive Spectroscopy (EDS)/mapping	1151:1194	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	5	35	dep	Spectroscopy	887:898	arg1	tests					941:945	tests	941:945	tests	941:945	Besides, the Electrochemical Impedance Spectroscopy (EIS) and polarization (potentiodynamic) tests were carried out for investigating the inhibition impacts of the constructed containers.
34225075	6	36	theme	Energy	1151:1156	arg1	EDS					1183:1185	EDS	1183:1185	EDS	1183:1185	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	6	36	theme	Energy	1151:1156	arg1	Spectroscopy					1169:1180	Energy Dispersive Spectroscopy	1151:1180	Energy Dispersive Spectroscopy (EDS)/mapping	1151:1194	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	11	37	theme	Monte	1911:1915	arg1	calculations					1950:1961	The Monte Carlo/Molecular Dynamics (MC/MD) calculations	1907:1961	The Monte Carlo/Molecular Dynamics (MC/MD) calculations	1907:1961	The Monte Carlo/Molecular Dynamics (MC/MD) calculations strongly affirmed the adsorption of BM and β-CD-BM over the substrate.
34225075	1	38	theme	NaCl	246:249	arg1	solutions					251:259	NaCl solutions	246:259	NaCl solutions	246:259	HYPOTHESIS Silane (sol-gel)-based coatings have been introduced as an eco-friendly system for reducing the metals' corrosion in NaCl solutions.
34225075	9	39	theme	composite	1645:1653	arg1	resistance					1618:1627	the total resistance	1608:1627	the total resistance of the modified composite	1608:1653	The EIS results also displayed that the total resistance of the modified composite was enhanced from 5540 Ω.cm2 to 10967 Ω.cm2 and the intact coating provided a total resistance of 80254 Ω.cm2.
34225075	7	40	theme	EIS	1268:1270	arg1	test					1272:1275	the EIS test	1264:1275	the EIS test	1264:1275	Also, the EIS test was conducted over the Silane-based composite film (SCF) for analyzing the anti-corrosion performance of the constructed composites.
34225075	10	41	theme	Density	1791:1797	arg1	explorations					1825:1836	The dispersion-corrected Density Functional Theory (DFT)-D explorations	1766:1836	The dispersion-corrected Density Functional Theory (DFT)-D explorations ascertained the inclusion capacity of benzimidazole	1766:1888	The dispersion-corrected Density Functional Theory (DFT)-D explorations ascertained the inclusion capacity of benzimidazole inside the β-CD.
34225075	3	42	theme	active	585:590	arg1	property					603:610	reliable active protection property	576:610	reliable active protection property	576:610	The present study introduces Beta-cyclodextrin (β-CD) as a novel/effective organic nano-container for Benzimidazole (BM) encapsulation to obtain reliable active protection property via a controlled-release property.
34225075	6	43	theme	incidence	1209:1217	arg1	GIXRD					1238:1242	GIXRD	1238:1242	GIXRD	1238:1242	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	6	43	theme	incidence	1209:1217	arg1	diffraction					1225:1235	Grazing incidence X-ray diffraction	1201:1235	Grazing incidence X-ray diffraction (GIXRD) experiments	1201:1255	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	3	44	theme	novel/effective	490:504	arg1	Beta-cyclodextrin					460:476	Beta-cyclodextrin	460:476	Beta-cyclodextrin (β-CD)	460:483	The present study introduces Beta-cyclodextrin (β-CD) as a novel/effective organic nano-container for Benzimidazole (BM) encapsulation to obtain reliable active protection property via a controlled-release property.
34225075	3	44	theme	novel/effective	490:504	arg1	nano-container					514:527	a novel/effective organic nano-container	488:527	a novel/effective organic nano-container for Benzimidazole (BM) encapsulation to obtain reliable active protection property via a controlled-release property	488:644	The present study introduces Beta-cyclodextrin (β-CD) as a novel/effective organic nano-container for Benzimidazole (BM) encapsulation to obtain reliable active protection property via a controlled-release property.
34225075	8	45	dep	FINDINGS	1410:1417	arg1	achievements					1427:1438	The EIS achievements	1419:1438	FINDINGS The EIS achievements	1410:1438	FINDINGS The EIS achievements demonstrated that by the addition of β-CD-BM complexes to the saline solution, the mild steel corrosion was mitigated by about 84%.
34225075	4	46	theme	β-CD-BM	689:695	arg1	macromolecule					697:709	the β-CD-BM macromolecule	685:709	the β-CD-BM macromolecule	685:709	EXPERIMENTS The chemical structure of the β-CD-BM macromolecule was explored by Fourier-transform infrared spectroscopy (FT-IR), X-Ray diffraction (XRD), and Ultraviolet-visible spectroscopy (UV-Vis).
34225075	10	47	theme	DFT	1818:1820	arg1	explorations					1825:1836	The dispersion-corrected Density Functional Theory (DFT)-D explorations	1766:1836	The dispersion-corrected Density Functional Theory (DFT)-D explorations ascertained the inclusion capacity of benzimidazole	1766:1888	The dispersion-corrected Density Functional Theory (DFT)-D explorations ascertained the inclusion capacity of benzimidazole inside the β-CD.
34225075	2	48	theme	protection	297:306	arg1	property					308:315	active protection property	290:315	active protection property	290:315	However, due to the lack of active protection property for this type of coatings, their modification is totally recommended for achieving durable protection properties.
34225075	5	49	theme	Electrochemical	861:875	arg1	Spectroscopy					887:898	the Electrochemical Impedance Spectroscopy	857:898	the Electrochemical Impedance Spectroscopy (EIS)	857:904	Besides, the Electrochemical Impedance Spectroscopy (EIS) and polarization (potentiodynamic) tests were carried out for investigating the inhibition impacts of the constructed containers.
34225075	5	49	theme	Electrochemical	861:875	arg1	EIS					901:903	EIS	901:903	EIS	901:903	Besides, the Electrochemical Impedance Spectroscopy (EIS) and polarization (potentiodynamic) tests were carried out for investigating the inhibition impacts of the constructed containers.
34225075	3	50	theme	protection	592:601	arg1	property					603:610	reliable active protection property	576:610	reliable active protection property	576:610	The present study introduces Beta-cyclodextrin (β-CD) as a novel/effective organic nano-container for Benzimidazole (BM) encapsulation to obtain reliable active protection property via a controlled-release property.
34225075	8	51	theme	β-CD-BM	1477:1483	arg1	complexes					1485:1493	β-CD-BM complexes	1477:1493	β-CD-BM complexes	1477:1493	FINDINGS The EIS achievements demonstrated that by the addition of β-CD-BM complexes to the saline solution, the mild steel corrosion was mitigated by about 84%.
34225075	4	52	theme	chemical	663:670	arg1	structure					672:680	The chemical structure	659:680	EXPERIMENTS The chemical structure of the β-CD-BM macromolecule	647:709	EXPERIMENTS The chemical structure of the β-CD-BM macromolecule was explored by Fourier-transform infrared spectroscopy (FT-IR), X-Ray diffraction (XRD), and Ultraviolet-visible spectroscopy (UV-Vis).
34225075	7	53	theme	Silane-based	1300:1311	arg1	SCF					1329:1331	SCF	1329:1331	SCF	1329:1331	Also, the EIS test was conducted over the Silane-based composite film (SCF) for analyzing the anti-corrosion performance of the constructed composites.
34225075	7	53	theme	Silane-based	1300:1311	arg1	film					1323:1326	the Silane-based composite film	1296:1326	the Silane-based composite film (SCF)	1296:1332	Also, the EIS test was conducted over the Silane-based composite film (SCF) for analyzing the anti-corrosion performance of the constructed composites.
34225075	11	54	theme	BM	1999:2000	arg1	adsorption					1985:1994	the adsorption	1981:1994	the adsorption of BM and β-CD-BM over the substrate	1981:2031	The Monte Carlo/Molecular Dynamics (MC/MD) calculations strongly affirmed the adsorption of BM and β-CD-BM over the substrate.
34225075	10	55	theme	-D	1822:1823	arg1	explorations					1825:1836	The dispersion-corrected Density Functional Theory (DFT)-D explorations	1766:1836	The dispersion-corrected Density Functional Theory (DFT)-D explorations ascertained the inclusion capacity of benzimidazole	1766:1888	The dispersion-corrected Density Functional Theory (DFT)-D explorations ascertained the inclusion capacity of benzimidazole inside the β-CD.
34225075	1	56	theme	sol-gel	137:143	arg1	system					201:206	an eco-friendly system	185:206	an eco-friendly system for reducing the metals' corrosion in NaCl solutions	185:259	HYPOTHESIS Silane (sol-gel)-based coatings have been introduced as an eco-friendly system for reducing the metals' corrosion in NaCl solutions.
34225075	1	56	theme	sol-gel	137:143	arg1	coatings					152:159	HYPOTHESIS Silane (sol-gel)-based coatings	118:159	HYPOTHESIS Silane (sol-gel)-based coatings	118:159	HYPOTHESIS Silane (sol-gel)-based coatings have been introduced as an eco-friendly system for reducing the metals' corrosion in NaCl solutions.
34225075	8	57	theme	saline	1502:1507	arg1	solution					1509:1516	the saline solution	1498:1516	the saline solution	1498:1516	FINDINGS The EIS achievements demonstrated that by the addition of β-CD-BM complexes to the saline solution, the mild steel corrosion was mitigated by about 84%.
34225075	7	58	theme	composites	1398:1407	arg1	performance					1367:1377	the anti-corrosion performance	1348:1377	the anti-corrosion performance of the constructed composites	1348:1407	Also, the EIS test was conducted over the Silane-based composite film (SCF) for analyzing the anti-corrosion performance of the constructed composites.
34225075	1	59	from	corrosion	233:241	arg1	solutions					251:259	NaCl solutions	246:259	NaCl solutions	246:259	HYPOTHESIS Silane (sol-gel)-based coatings have been introduced as an eco-friendly system for reducing the metals' corrosion in NaCl solutions.
34225075	4	60	dep	EXPERIMENTS	647:657	arg1	structure					672:680	The chemical structure	659:680	EXPERIMENTS The chemical structure of the β-CD-BM macromolecule	647:709	EXPERIMENTS The chemical structure of the β-CD-BM macromolecule was explored by Fourier-transform infrared spectroscopy (FT-IR), X-Ray diffraction (XRD), and Ultraviolet-visible spectroscopy (UV-Vis).
34225075	1	61	theme	-based	145:150	arg1	system					201:206	an eco-friendly system	185:206	an eco-friendly system for reducing the metals' corrosion in NaCl solutions	185:259	HYPOTHESIS Silane (sol-gel)-based coatings have been introduced as an eco-friendly system for reducing the metals' corrosion in NaCl solutions.
34225075	1	61	theme	-based	145:150	arg1	coatings					152:159	HYPOTHESIS Silane (sol-gel)-based coatings	118:159	HYPOTHESIS Silane (sol-gel)-based coatings	118:159	HYPOTHESIS Silane (sol-gel)-based coatings have been introduced as an eco-friendly system for reducing the metals' corrosion in NaCl solutions.
34225075	10	62	theme	Theory	1810:1815	arg1	explorations					1825:1836	The dispersion-corrected Density Functional Theory (DFT)-D explorations	1766:1836	The dispersion-corrected Density Functional Theory (DFT)-D explorations ascertained the inclusion capacity of benzimidazole	1766:1888	The dispersion-corrected Density Functional Theory (DFT)-D explorations ascertained the inclusion capacity of benzimidazole inside the β-CD.
34225075	9	63	theme	80254 Ω.cm2	1753:1763	arg1	resistance					1739:1748	a total resistance	1731:1748	a total resistance of 80254 Ω.cm2	1731:1763	The EIS results also displayed that the total resistance of the modified composite was enhanced from 5540 Ω.cm2 to 10967 Ω.cm2 and the intact coating provided a total resistance of 80254 Ω.cm2.
34225075	11	64	theme	β-CD-BM	2006:2012	arg1	adsorption					1985:1994	the adsorption	1981:1994	the adsorption of BM and β-CD-BM over the substrate	1981:2031	The Monte Carlo/Molecular Dynamics (MC/MD) calculations strongly affirmed the adsorption of BM and β-CD-BM over the substrate.
34225075	8	65	theme	mild	1523:1526	arg1	corrosion					1534:1542	the mild steel corrosion	1519:1542	the mild steel corrosion	1519:1542	FINDINGS The EIS achievements demonstrated that by the addition of β-CD-BM complexes to the saline solution, the mild steel corrosion was mitigated by about 84%.
34225075	6	66	theme	diffraction	1225:1235	arg1	experiments					1245:1255	Grazing incidence X-ray diffraction (GIXRD) experiments	1201:1255	Grazing incidence X-ray diffraction (GIXRD) experiments	1201:1255	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	4	67	theme	infrared	745:752	arg1	FT-IR					768:772	FT-IR	768:772	FT-IR	768:772	EXPERIMENTS The chemical structure of the β-CD-BM macromolecule was explored by Fourier-transform infrared spectroscopy (FT-IR), X-Ray diffraction (XRD), and Ultraviolet-visible spectroscopy (UV-Vis).
34225075	4	67	theme	infrared	745:752	arg1	spectroscopy					754:765	Fourier-transform infrared spectroscopy	727:765	Fourier-transform infrared spectroscopy (FT-IR)	727:773	EXPERIMENTS The chemical structure of the β-CD-BM macromolecule was explored by Fourier-transform infrared spectroscopy (FT-IR), X-Ray diffraction (XRD), and Ultraviolet-visible spectroscopy (UV-Vis).
34225075	10	68	theme	Functional	1799:1808	arg1	explorations					1825:1836	The dispersion-corrected Density Functional Theory (DFT)-D explorations	1766:1836	The dispersion-corrected Density Functional Theory (DFT)-D explorations ascertained the inclusion capacity of benzimidazole	1766:1888	The dispersion-corrected Density Functional Theory (DFT)-D explorations ascertained the inclusion capacity of benzimidazole inside the β-CD.
34225075	5	69	theme	constructed	1012:1022	arg1	containers					1024:1033	the constructed containers	1008:1033	the constructed containers	1008:1033	Besides, the Electrochemical Impedance Spectroscopy (EIS) and polarization (potentiodynamic) tests were carried out for investigating the inhibition impacts of the constructed containers.
34225075	9	70	theme	EIS	1576:1578	arg1	results					1580:1586	The EIS results	1572:1586	The EIS results	1572:1586	The EIS results also displayed that the total resistance of the modified composite was enhanced from 5540 Ω.cm2 to 10967 Ω.cm2 and the intact coating provided a total resistance of 80254 Ω.cm2.
34225075	6	71	theme	Scanning	1112:1119	arg1	FE-SEM					1142:1147	FE-SEM	1142:1147	FE-SEM	1142:1147	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	6	71	theme	Scanning	1112:1119	arg1	Microscope					1130:1139	Field Emission Scanning Electron Microscope	1097:1139	Field Emission Scanning Electron Microscope (FE-SEM)	1097:1148	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	4	72	theme	X-Ray	776:780	arg1	XRD					795:797	XRD	795:797	XRD	795:797	EXPERIMENTS The chemical structure of the β-CD-BM macromolecule was explored by Fourier-transform infrared spectroscopy (FT-IR), X-Ray diffraction (XRD), and Ultraviolet-visible spectroscopy (UV-Vis).
34225075	4	72	theme	X-Ray	776:780	arg1	diffraction					782:792	X-Ray diffraction	776:792	X-Ray diffraction (XRD)	776:798	EXPERIMENTS The chemical structure of the β-CD-BM macromolecule was explored by Fourier-transform infrared spectroscopy (FT-IR), X-Ray diffraction (XRD), and Ultraviolet-visible spectroscopy (UV-Vis).
34225075	3	73	theme	present	435:441	arg1	study					443:447	The present study	431:447	The present study	431:447	The present study introduces Beta-cyclodextrin (β-CD) as a novel/effective organic nano-container for Benzimidazole (BM) encapsulation to obtain reliable active protection property via a controlled-release property.
34225075	5	74	theme	inhibition	986:995	arg1	impacts					997:1003	the inhibition impacts	982:1003	the inhibition impacts of the constructed containers	982:1033	Besides, the Electrochemical Impedance Spectroscopy (EIS) and polarization (potentiodynamic) tests were carried out for investigating the inhibition impacts of the constructed containers.
34225075	6	75	theme	Field	1097:1101	arg1	FE-SEM					1142:1147	FE-SEM	1142:1147	FE-SEM	1142:1147	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	6	75	theme	Field	1097:1101	arg1	Microscope					1130:1139	Field Emission Scanning Electron Microscope	1097:1139	Field Emission Scanning Electron Microscope (FE-SEM)	1097:1148	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	3	76	theme	Benzimidazole	533:545	arg1	encapsulation					552:564	Benzimidazole (BM) encapsulation	533:564	Benzimidazole (BM) encapsulation	533:564	The present study introduces Beta-cyclodextrin (β-CD) as a novel/effective organic nano-container for Benzimidazole (BM) encapsulation to obtain reliable active protection property via a controlled-release property.
34225075	0	77	theme	novel	41:45	arg1	β-cyclodextrin					21:34	β-cyclodextrin	21:34	β-cyclodextrin	21:34	Benzimidazole loaded β-cyclodextrin as a novel anti-corrosion system; coupled experimental/computational assessments.
34225075	0	77	theme	novel	41:45	arg1	system					62:67	a novel anti-corrosion system	39:67	a novel anti-corrosion system	39:67	Benzimidazole loaded β-cyclodextrin as a novel anti-corrosion system; coupled experimental/computational assessments.
34225075	7	78	theme	composite	1313:1321	arg1	SCF					1329:1331	SCF	1329:1331	SCF	1329:1331	Also, the EIS test was conducted over the Silane-based composite film (SCF) for analyzing the anti-corrosion performance of the constructed composites.
34225075	7	78	theme	composite	1313:1321	arg1	film					1323:1326	the Silane-based composite film	1296:1326	the Silane-based composite film (SCF)	1296:1332	Also, the EIS test was conducted over the Silane-based composite film (SCF) for analyzing the anti-corrosion performance of the constructed composites.
34225075	2	79	theme	durable	400:406	arg1	properties					419:428	durable protection properties	400:428	durable protection properties	400:428	However, due to the lack of active protection property for this type of coatings, their modification is totally recommended for achieving durable protection properties.
34225075	9	80	theme	total	1612:1616	arg1	resistance					1618:1627	the total resistance	1608:1627	the total resistance of the modified composite	1608:1653	The EIS results also displayed that the total resistance of the modified composite was enhanced from 5540 Ω.cm2 to 10967 Ω.cm2 and the intact coating provided a total resistance of 80254 Ω.cm2.
34225075	3	81	theme	organic	506:512	arg1	Beta-cyclodextrin					460:476	Beta-cyclodextrin	460:476	Beta-cyclodextrin (β-CD)	460:483	The present study introduces Beta-cyclodextrin (β-CD) as a novel/effective organic nano-container for Benzimidazole (BM) encapsulation to obtain reliable active protection property via a controlled-release property.
34225075	3	81	theme	organic	506:512	arg1	nano-container					514:527	a novel/effective organic nano-container	488:527	a novel/effective organic nano-container for Benzimidazole (BM) encapsulation to obtain reliable active protection property via a controlled-release property	488:644	The present study introduces Beta-cyclodextrin (β-CD) as a novel/effective organic nano-container for Benzimidazole (BM) encapsulation to obtain reliable active protection property via a controlled-release property.
34225075	2	82	theme	active	290:295	arg1	property					308:315	active protection property	290:315	active protection property	290:315	However, due to the lack of active protection property for this type of coatings, their modification is totally recommended for achieving durable protection properties.
34225075	6	83	theme	Dispersive	1158:1167	arg1	EDS					1183:1185	EDS	1183:1185	EDS	1183:1185	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	6	83	theme	Dispersive	1158:1167	arg1	Spectroscopy					1169:1180	Energy Dispersive Spectroscopy	1151:1180	Energy Dispersive Spectroscopy (EDS)/mapping	1151:1194	The exposed and unexposed samples' surfaces were analyzed by Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive Spectroscopy (EDS)/mapping, and Grazing incidence X-ray diffraction (GIXRD) experiments.
34225075	9	84	theme	modified	1636:1643	arg1	composite					1645:1653	the modified composite	1632:1653	the modified composite	1632:1653	The EIS results also displayed that the total resistance of the modified composite was enhanced from 5540 Ω.cm2 to 10967 Ω.cm2 and the intact coating provided a total resistance of 80254 Ω.cm2.
34225075	11	85	theme	Carlo/Molecular	1917:1931	arg1	calculations					1950:1961	The Monte Carlo/Molecular Dynamics (MC/MD) calculations	1907:1961	The Monte Carlo/Molecular Dynamics (MC/MD) calculations	1907:1961	The Monte Carlo/Molecular Dynamics (MC/MD) calculations strongly affirmed the adsorption of BM and β-CD-BM over the substrate.
32344350	0	0	theme	physicochemical	82:96	arg1	properties					98:107	physicochemical properties	82:107	physicochemical properties	82:107	Effect of freeze-thaw cycles pretreatment on the vacuum freeze-drying process and physicochemical properties of the dried garlic slices.
32344350	2	1	theme	water	328:332	arg1	status					334:339	water status	328:339	water status	328:339	Cell viability, water status, internal structure, flavor, chemical composition and thermogravimetric of garlic samples were evaluated.
32344350	4	2	theme	thermal	698:704	arg1	stability					706:714	thermal stability	698:714	thermal stability	698:714	The FTC pretreatment improved thermal stability, flavor and chemical composition content of dried products.
32344350	3	3	theme	drying	503:508	arg1	%					528:528	22.22%-33.33%	516:528	22.22%-33.33%	516:528	The results indicated that FTC pretreatment reduced the drying time (22.22%-33.33%) and the energy consumption (14.25%-15.50%), owing to the water loss, the increase in free water, and the formation of porous structures.
32344350	3	3	theme	drying	503:508	arg1	time					510:513	the drying time	499:513	the drying time (22.22%-33.33%)	499:529	The results indicated that FTC pretreatment reduced the drying time (22.22%-33.33%) and the energy consumption (14.25%-15.50%), owing to the water loss, the increase in free water, and the formation of porous structures.
32344350	3	4	theme	%	521:521	arg1	%					528:528	22.22%-33.33%	516:528	22.22%-33.33%	516:528	The results indicated that FTC pretreatment reduced the drying time (22.22%-33.33%) and the energy consumption (14.25%-15.50%), owing to the water loss, the increase in free water, and the formation of porous structures.
32344350	3	4	theme	%	521:521	arg1	time					510:513	the drying time	499:513	the drying time (22.22%-33.33%)	499:529	The results indicated that FTC pretreatment reduced the drying time (22.22%-33.33%) and the energy consumption (14.25%-15.50%), owing to the water loss, the increase in free water, and the formation of porous structures.
32344350	5	5	theme	unpretreated	893:904	arg1	product					912:918	the unpretreated dried product	889:918	the unpretreated dried product due to the reduction in polysaccharide molecular weight	889:974	The antioxidant activity of polysaccharides extracted from FTC pretreated dried products was higher than that of the unpretreated dried product due to the reduction in polysaccharide molecular weight.
32344350	1	6	theme	drying	261:266	arg1	process					268:274	the drying process	257:274	the drying process	257:274	Freeze-thaw cycles (FTC) pretreatment was employed before the vacuum freeze-drying of garlic slices, aimed at improving the drying process and the quality of the end product.
32344350	0	7	theme	dried	116:120	arg1	slices					129:134	the dried garlic slices	112:134	the dried garlic slices	112:134	Effect of freeze-thaw cycles pretreatment on the vacuum freeze-drying process and physicochemical properties of the dried garlic slices.
32344350	5	8	theme	pretreated	839:848	arg1	products					856:863	FTC pretreated dried products	835:863	FTC pretreated dried products	835:863	The antioxidant activity of polysaccharides extracted from FTC pretreated dried products was higher than that of the unpretreated dried product due to the reduction in polysaccharide molecular weight.
32344350	4	9	theme	dried	760:764	arg1	products					766:773	dried products	760:773	dried products	760:773	The FTC pretreatment improved thermal stability, flavor and chemical composition content of dried products.
32344350	5	10	theme	dried	906:910	arg1	product					912:918	the unpretreated dried product	889:918	the unpretreated dried product due to the reduction in polysaccharide molecular weight	889:974	The antioxidant activity of polysaccharides extracted from FTC pretreated dried products was higher than that of the unpretreated dried product due to the reduction in polysaccharide molecular weight.
32344350	5	11	from	reduction	931:939	arg1	weight					969:974	polysaccharide molecular weight	944:974	polysaccharide molecular weight	944:974	The antioxidant activity of polysaccharides extracted from FTC pretreated dried products was higher than that of the unpretreated dried product due to the reduction in polysaccharide molecular weight.
32344350	6	12	theme	slices	1048:1053	arg1	production					1021:1030	future production	1014:1030	future production of dried garlic slices having good quality	1014:1073	This research could pave a route for future production of dried garlic slices having good quality by using the FTC pretreatment, with lower energy consumption and shorter drying time.
32344350	3	13	theme	22.22	516:520	arg1	%					528:528	22.22%-33.33%	516:528	22.22%-33.33%	516:528	The results indicated that FTC pretreatment reduced the drying time (22.22%-33.33%) and the energy consumption (14.25%-15.50%), owing to the water loss, the increase in free water, and the formation of porous structures.
32344350	3	13	theme	22.22	516:520	arg1	time					510:513	the drying time	499:513	the drying time (22.22%-33.33%)	499:529	The results indicated that FTC pretreatment reduced the drying time (22.22%-33.33%) and the energy consumption (14.25%-15.50%), owing to the water loss, the increase in free water, and the formation of porous structures.
32344350	6	14	theme	future	1014:1019	arg1	production					1021:1030	future production	1014:1030	future production of dried garlic slices having good quality	1014:1073	This research could pave a route for future production of dried garlic slices having good quality by using the FTC pretreatment, with lower energy consumption and shorter drying time.
32344350	2	15	theme	internal	342:349	arg1	structure					351:359	internal structure	342:359	internal structure	342:359	Cell viability, water status, internal structure, flavor, chemical composition and thermogravimetric of garlic samples were evaluated.
32344350	0	16	from	pretreatment	29:40	arg1	process					70:76	vacuum freeze-drying process	49:76	vacuum freeze-drying process	49:76	Effect of freeze-thaw cycles pretreatment on the vacuum freeze-drying process and physicochemical properties of the dried garlic slices.
32344350	0	16	from	pretreatment	29:40	arg1	properties					98:107	physicochemical properties	82:107	physicochemical properties	82:107	Effect of freeze-thaw cycles pretreatment on the vacuum freeze-drying process and physicochemical properties of the dried garlic slices.
32344350	2	17	theme	samples	423:429	arg1	flavor					362:367	flavor	362:367	flavor	362:367	Cell viability, water status, internal structure, flavor, chemical composition and thermogravimetric of garlic samples were evaluated.
32344350	2	17	theme	samples	423:429	arg1	status					334:339	water status	328:339	water status	328:339	Cell viability, water status, internal structure, flavor, chemical composition and thermogravimetric of garlic samples were evaluated.
32344350	2	17	theme	samples	423:429	arg1	composition					379:389	chemical composition	370:389	chemical composition	370:389	Cell viability, water status, internal structure, flavor, chemical composition and thermogravimetric of garlic samples were evaluated.
32344350	2	17	theme	samples	423:429	arg1	thermogravimetric					395:411	thermogravimetric	395:411	thermogravimetric	395:411	Cell viability, water status, internal structure, flavor, chemical composition and thermogravimetric of garlic samples were evaluated.
32344350	2	17	theme	samples	423:429	arg1	viability					317:325	Cell viability	312:325	Cell viability	312:325	Cell viability, water status, internal structure, flavor, chemical composition and thermogravimetric of garlic samples were evaluated.
32344350	2	17	theme	samples	423:429	arg1	structure					351:359	internal structure	342:359	internal structure	342:359	Cell viability, water status, internal structure, flavor, chemical composition and thermogravimetric of garlic samples were evaluated.
32344350	3	18	theme	structures	656:665	arg1	loss					594:597	the water loss	584:597	the water loss	584:597	The results indicated that FTC pretreatment reduced the drying time (22.22%-33.33%) and the energy consumption (14.25%-15.50%), owing to the water loss, the increase in free water, and the formation of porous structures.
32344350	3	18	theme	structures	656:665	arg1	increase					604:611	the increase	600:611	the increase in free water	600:625	The results indicated that FTC pretreatment reduced the drying time (22.22%-33.33%) and the energy consumption (14.25%-15.50%), owing to the water loss, the increase in free water, and the formation of porous structures.
32344350	3	18	theme	structures	656:665	arg1	formation					636:644	the formation	632:644	the formation of porous structures	632:665	The results indicated that FTC pretreatment reduced the drying time (22.22%-33.33%) and the energy consumption (14.25%-15.50%), owing to the water loss, the increase in free water, and the formation of porous structures.
32344350	4	19	theme	products	766:773	arg1	flavor					717:722	flavor	717:722	flavor	717:722	The FTC pretreatment improved thermal stability, flavor and chemical composition content of dried products.
32344350	4	19	theme	products	766:773	arg1	stability					706:714	thermal stability	698:714	thermal stability	698:714	The FTC pretreatment improved thermal stability, flavor and chemical composition content of dried products.
32344350	4	19	theme	products	766:773	arg1	content					749:755	chemical composition content	728:755	chemical composition content	728:755	The FTC pretreatment improved thermal stability, flavor and chemical composition content of dried products.
32344350	0	20	theme	cycles	22:27	arg1	Effect					0:5	Effect	0:5	Effect of freeze-thaw cycles	0:27	Effect of freeze-thaw cycles pretreatment on the vacuum freeze-drying process and physicochemical properties of the dried garlic slices.
32344350	6	21	theme	energy	1117:1122	arg1	consumption					1124:1134	lower energy consumption	1111:1134	lower energy consumption	1111:1134	This research could pave a route for future production of dried garlic slices having good quality by using the FTC pretreatment, with lower energy consumption and shorter drying time.
32344350	0	22	theme	slices	129:134	arg1	process					70:76	vacuum freeze-drying process	49:76	vacuum freeze-drying process	49:76	Effect of freeze-thaw cycles pretreatment on the vacuum freeze-drying process and physicochemical properties of the dried garlic slices.
32344350	0	22	theme	slices	129:134	arg1	properties					98:107	physicochemical properties	82:107	physicochemical properties	82:107	Effect of freeze-thaw cycles pretreatment on the vacuum freeze-drying process and physicochemical properties of the dried garlic slices.
32344350	6	23	theme	shorter	1140:1146	arg1	time					1155:1158	shorter drying time	1140:1158	shorter drying time	1140:1158	This research could pave a route for future production of dried garlic slices having good quality by using the FTC pretreatment, with lower energy consumption and shorter drying time.
32344350	0	24	dep	process	70:76	arg1	the					45:47	the	45:47	the	45:47	Effect of freeze-thaw cycles pretreatment on the vacuum freeze-drying process and physicochemical properties of the dried garlic slices.
32344350	0	25	theme	freeze-thaw	10:20	arg1	cycles					22:27	freeze-thaw cycles	10:27	freeze-thaw cycles	10:27	Effect of freeze-thaw cycles pretreatment on the vacuum freeze-drying process and physicochemical properties of the dried garlic slices.
32344350	0	26	theme	garlic	122:127	arg1	slices					129:134	the dried garlic slices	112:134	the dried garlic slices	112:134	Effect of freeze-thaw cycles pretreatment on the vacuum freeze-drying process and physicochemical properties of the dried garlic slices.
32344350	5	27	theme	FTC	835:837	arg1	products					856:863	FTC pretreated dried products	835:863	FTC pretreated dried products	835:863	The antioxidant activity of polysaccharides extracted from FTC pretreated dried products was higher than that of the unpretreated dried product due to the reduction in polysaccharide molecular weight.
32344350	5	28	theme	molecular	959:967	arg1	weight					969:974	polysaccharide molecular weight	944:974	polysaccharide molecular weight	944:974	The antioxidant activity of polysaccharides extracted from FTC pretreated dried products was higher than that of the unpretreated dried product due to the reduction in polysaccharide molecular weight.
32344350	1	29	theme	vacuum	199:204	arg1	freeze-drying					206:218	the vacuum freeze-drying	195:218	the vacuum freeze-drying of garlic slices, aimed at improving the drying process	195:274	Freeze-thaw cycles (FTC) pretreatment was employed before the vacuum freeze-drying of garlic slices, aimed at improving the drying process and the quality of the end product.
32344350	3	30	from	increase	604:611	arg1	water					621:625	free water	616:625	free water	616:625	The results indicated that FTC pretreatment reduced the drying time (22.22%-33.33%) and the energy consumption (14.25%-15.50%), owing to the water loss, the increase in free water, and the formation of porous structures.
32344350	3	31	theme	porous	649:654	arg1	structures					656:665	porous structures	649:665	porous structures	649:665	The results indicated that FTC pretreatment reduced the drying time (22.22%-33.33%) and the energy consumption (14.25%-15.50%), owing to the water loss, the increase in free water, and the formation of porous structures.
32344350	6	32	theme	drying	1148:1153	arg1	time					1155:1158	shorter drying time	1140:1158	shorter drying time	1140:1158	This research could pave a route for future production of dried garlic slices having good quality by using the FTC pretreatment, with lower energy consumption and shorter drying time.
32344350	4	33	theme	chemical	728:735	arg1	content					749:755	chemical composition content	728:755	chemical composition content	728:755	The FTC pretreatment improved thermal stability, flavor and chemical composition content of dried products.
32344350	6	34	theme	lower	1111:1115	arg1	consumption					1124:1134	lower energy consumption	1111:1134	lower energy consumption	1111:1134	This research could pave a route for future production of dried garlic slices having good quality by using the FTC pretreatment, with lower energy consumption and shorter drying time.
32344350	6	35	contain	having	1055:1060	arg1	slices					1048:1053	dried garlic slices	1035:1053	dried garlic slices having good quality	1035:1073	This research could pave a route for future production of dried garlic slices having good quality by using the FTC pretreatment, with lower energy consumption and shorter drying time.
32344350	6	35	contain	having	1055:1060	arg2	quality					1067:1073	good quality	1062:1073	good quality	1062:1073	This research could pave a route for future production of dried garlic slices having good quality by using the FTC pretreatment, with lower energy consumption and shorter drying time.
32344350	2	36	theme	garlic	416:421	arg1	samples					423:429	garlic samples	416:429	garlic samples	416:429	Cell viability, water status, internal structure, flavor, chemical composition and thermogravimetric of garlic samples were evaluated.
32344350	2	37	theme	chemical	370:377	arg1	composition					379:389	chemical composition	370:389	chemical composition	370:389	Cell viability, water status, internal structure, flavor, chemical composition and thermogravimetric of garlic samples were evaluated.
32344350	0	38	theme	vacuum	49:54	arg1	process					70:76	vacuum freeze-drying process	49:76	vacuum freeze-drying process	49:76	Effect of freeze-thaw cycles pretreatment on the vacuum freeze-drying process and physicochemical properties of the dried garlic slices.
32344350	5	39	theme	polysaccharides	804:818	arg1	higher					869:874	higher	869:874	higher	869:874	The antioxidant activity of polysaccharides extracted from FTC pretreated dried products was higher than that of the unpretreated dried product due to the reduction in polysaccharide molecular weight.
32344350	5	39	theme	polysaccharides	804:818	arg1	activity					792:799	The antioxidant activity	776:799	The antioxidant activity of polysaccharides extracted from FTC pretreated dried products	776:863	The antioxidant activity of polysaccharides extracted from FTC pretreated dried products was higher than that of the unpretreated dried product due to the reduction in polysaccharide molecular weight.
32344350	3	40	from	loss	594:597	arg1	water					621:625	free water	616:625	free water	616:625	The results indicated that FTC pretreatment reduced the drying time (22.22%-33.33%) and the energy consumption (14.25%-15.50%), owing to the water loss, the increase in free water, and the formation of porous structures.
32344350	3	41	theme	energy	539:544	arg1	%					571:571	14.25%-15.50%	559:571	14.25%-15.50%	559:571	The results indicated that FTC pretreatment reduced the drying time (22.22%-33.33%) and the energy consumption (14.25%-15.50%), owing to the water loss, the increase in free water, and the formation of porous structures.
32344350	3	41	theme	energy	539:544	arg1	consumption					546:556	the energy consumption	535:556	the energy consumption (14.25%-15.50%)	535:572	The results indicated that FTC pretreatment reduced the drying time (22.22%-33.33%) and the energy consumption (14.25%-15.50%), owing to the water loss, the increase in free water, and the formation of porous structures.
32344350	6	42	theme	dried	1035:1039	arg1	slices					1048:1053	dried garlic slices	1035:1053	dried garlic slices having good quality	1035:1073	This research could pave a route for future production of dried garlic slices having good quality by using the FTC pretreatment, with lower energy consumption and shorter drying time.
32344350	3	43	theme	FTC	474:476	arg1	pretreatment					478:489	FTC pretreatment	474:489	FTC pretreatment	474:489	The results indicated that FTC pretreatment reduced the drying time (22.22%-33.33%) and the energy consumption (14.25%-15.50%), owing to the water loss, the increase in free water, and the formation of porous structures.
32344350	1	44	theme	end	299:301	arg1	product					303:309	the end product	295:309	the end product	295:309	Freeze-thaw cycles (FTC) pretreatment was employed before the vacuum freeze-drying of garlic slices, aimed at improving the drying process and the quality of the end product.
32344350	4	45	theme	composition	737:747	arg1	content					749:755	chemical composition content	728:755	chemical composition content	728:755	The FTC pretreatment improved thermal stability, flavor and chemical composition content of dried products.
32344350	5	46	theme	dried	850:854	arg1	products					856:863	FTC pretreated dried products	835:863	FTC pretreated dried products	835:863	The antioxidant activity of polysaccharides extracted from FTC pretreated dried products was higher than that of the unpretreated dried product due to the reduction in polysaccharide molecular weight.
32344350	1	47	theme	product	303:309	arg1	quality					284:290	the quality	280:290	the quality of the end product	280:309	Freeze-thaw cycles (FTC) pretreatment was employed before the vacuum freeze-drying of garlic slices, aimed at improving the drying process and the quality of the end product.
32344350	1	47	theme	product	303:309	arg1	freeze-drying					206:218	the vacuum freeze-drying	195:218	the vacuum freeze-drying of garlic slices, aimed at improving the drying process	195:274	Freeze-thaw cycles (FTC) pretreatment was employed before the vacuum freeze-drying of garlic slices, aimed at improving the drying process and the quality of the end product.
32344350	5	48	theme	due	920:922	arg1	product					912:918	the unpretreated dried product	889:918	the unpretreated dried product due to the reduction in polysaccharide molecular weight	889:974	The antioxidant activity of polysaccharides extracted from FTC pretreated dried products was higher than that of the unpretreated dried product due to the reduction in polysaccharide molecular weight.
32344350	5	49	theme	polysaccharide	944:957	arg1	weight					969:974	polysaccharide molecular weight	944:974	polysaccharide molecular weight	944:974	The antioxidant activity of polysaccharides extracted from FTC pretreated dried products was higher than that of the unpretreated dried product due to the reduction in polysaccharide molecular weight.
32344350	2	50	theme	Cell	312:315	arg1	viability					317:325	Cell viability	312:325	Cell viability	312:325	Cell viability, water status, internal structure, flavor, chemical composition and thermogravimetric of garlic samples were evaluated.
32344350	6	51	theme	garlic	1041:1046	arg1	slices					1048:1053	dried garlic slices	1035:1053	dried garlic slices having good quality	1035:1073	This research could pave a route for future production of dried garlic slices having good quality by using the FTC pretreatment, with lower energy consumption and shorter drying time.
32344350	1	52	theme	garlic	223:228	arg1	slices					230:235	garlic slices	223:235	garlic slices	223:235	Freeze-thaw cycles (FTC) pretreatment was employed before the vacuum freeze-drying of garlic slices, aimed at improving the drying process and the quality of the end product.
32344350	6	53	theme	FTC	1088:1090	arg1	pretreatment					1092:1103	the FTC pretreatment	1084:1103	the FTC pretreatment	1084:1103	This research could pave a route for future production of dried garlic slices having good quality by using the FTC pretreatment, with lower energy consumption and shorter drying time.
32344350	3	54	theme	water	588:592	arg1	loss					594:597	the water loss	584:597	the water loss	584:597	The results indicated that FTC pretreatment reduced the drying time (22.22%-33.33%) and the energy consumption (14.25%-15.50%), owing to the water loss, the increase in free water, and the formation of porous structures.
32344350	4	55	theme	FTC	672:674	arg1	pretreatment					676:687	The FTC pretreatment	668:687	The FTC pretreatment	668:687	The FTC pretreatment improved thermal stability, flavor and chemical composition content of dried products.
32344350	1	56	theme	Freeze-thaw	137:147	arg1	FTC					157:159	FTC	157:159	FTC	157:159	Freeze-thaw cycles (FTC) pretreatment was employed before the vacuum freeze-drying of garlic slices, aimed at improving the drying process and the quality of the end product.
32344350	1	56	theme	Freeze-thaw	137:147	arg1	cycles					149:154	Freeze-thaw cycles	137:154	Freeze-thaw cycles (FTC) pretreatment	137:173	Freeze-thaw cycles (FTC) pretreatment was employed before the vacuum freeze-drying of garlic slices, aimed at improving the drying process and the quality of the end product.
32344350	6	57	theme	good	1062:1065	arg1	quality					1067:1073	good quality	1062:1073	good quality	1062:1073	This research could pave a route for future production of dried garlic slices having good quality by using the FTC pretreatment, with lower energy consumption and shorter drying time.
32344350	1	58	theme	slices	230:235	arg1	quality					284:290	the quality	280:290	the quality of the end product	280:309	Freeze-thaw cycles (FTC) pretreatment was employed before the vacuum freeze-drying of garlic slices, aimed at improving the drying process and the quality of the end product.
32344350	1	58	theme	slices	230:235	arg1	freeze-drying					206:218	the vacuum freeze-drying	195:218	the vacuum freeze-drying of garlic slices, aimed at improving the drying process	195:274	Freeze-thaw cycles (FTC) pretreatment was employed before the vacuum freeze-drying of garlic slices, aimed at improving the drying process and the quality of the end product.
32344350	3	59	theme	%	564:564	arg1	%					571:571	14.25%-15.50%	559:571	14.25%-15.50%	559:571	The results indicated that FTC pretreatment reduced the drying time (22.22%-33.33%) and the energy consumption (14.25%-15.50%), owing to the water loss, the increase in free water, and the formation of porous structures.
32344350	3	59	theme	%	564:564	arg1	consumption					546:556	the energy consumption	535:556	the energy consumption (14.25%-15.50%)	535:572	The results indicated that FTC pretreatment reduced the drying time (22.22%-33.33%) and the energy consumption (14.25%-15.50%), owing to the water loss, the increase in free water, and the formation of porous structures.
32344350	1	60	theme	cycles	149:154	arg1	pretreatment					162:173	Freeze-thaw cycles (FTC) pretreatment	137:173	Freeze-thaw cycles (FTC) pretreatment	137:173	Freeze-thaw cycles (FTC) pretreatment was employed before the vacuum freeze-drying of garlic slices, aimed at improving the drying process and the quality of the end product.
32344350	5	61	theme	antioxidant	780:790	arg1	higher					869:874	higher	869:874	higher	869:874	The antioxidant activity of polysaccharides extracted from FTC pretreated dried products was higher than that of the unpretreated dried product due to the reduction in polysaccharide molecular weight.
32344350	5	61	theme	antioxidant	780:790	arg1	activity					792:799	The antioxidant activity	776:799	The antioxidant activity of polysaccharides extracted from FTC pretreated dried products	776:863	The antioxidant activity of polysaccharides extracted from FTC pretreated dried products was higher than that of the unpretreated dried product due to the reduction in polysaccharide molecular weight.
32344350	0	62	theme	freeze-drying	56:68	arg1	process					70:76	vacuum freeze-drying process	49:76	vacuum freeze-drying process	49:76	Effect of freeze-thaw cycles pretreatment on the vacuum freeze-drying process and physicochemical properties of the dried garlic slices.
32344350	3	63	theme	free	616:619	arg1	water					621:625	free water	616:625	free water	616:625	The results indicated that FTC pretreatment reduced the drying time (22.22%-33.33%) and the energy consumption (14.25%-15.50%), owing to the water loss, the increase in free water, and the formation of porous structures.
32344350	3	64	from	formation	636:644	arg1	water					621:625	free water	616:625	free water	616:625	The results indicated that FTC pretreatment reduced the drying time (22.22%-33.33%) and the energy consumption (14.25%-15.50%), owing to the water loss, the increase in free water, and the formation of porous structures.
33960765	9	0	theme	biomaterial	1759:1769	arg1	promising					1859:1867	promising	1859:1867	promising	1859:1867	Together, the printable biomaterial inks with shear thinning and good structural integrity, along with biological cues, are promising for tissue engineering application.
33960765	9	0	theme	biomaterial	1759:1769	arg1	inks					1771:1774	the printable biomaterial inks	1745:1774	the printable biomaterial inks with shear thinning and good structural integrity	1745:1824	Together, the printable biomaterial inks with shear thinning and good structural integrity, along with biological cues, are promising for tissue engineering application.
33960765	6	1	theme	printed	1207:1213	arg1	constructs					1215:1224	the printed constructs	1203:1224	the printed constructs	1203:1224	The rheological behavior of the hydrogels and the mechanical strengths of the printed constructs are tuned by adjusting the content of GelMA, which in turn enhances the shape retention after printing and enables the precise deposition of multilayered 3D scaffolds.
33960765	3	2	theme	suitable	586:593	arg1	printability					595:606	suitable printability	586:606	suitable printability	586:606	However, due to the lack of bioinks with suitable printability, high structural integrity, and biological compatibility, producing constructs that mimic the anisotropic 3D extracellular environments remains a challenge.
33960765	2	3	theme	Three-dimensional	283:299	arg1	printing					306:313	Three-dimensional (3D) printing	283:313	Three-dimensional (3D) printing	283:313	Three-dimensional (3D) printing is an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold.
33960765	2	3	theme	Three-dimensional	283:299	arg1	method					332:337	an innovative method	318:337	an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold	318:542	Three-dimensional (3D) printing is an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold.
33960765	6	4	theme	3D	1380:1381	arg1	scaffolds					1383:1391	multilayered 3D scaffolds	1367:1391	multilayered 3D scaffolds	1367:1391	The rheological behavior of the hydrogels and the mechanical strengths of the printed constructs are tuned by adjusting the content of GelMA, which in turn enhances the shape retention after printing and enables the precise deposition of multilayered 3D scaffolds.
33960765	2	5	theme	innovative	321:330	arg1	printing					306:313	Three-dimensional (3D) printing	283:313	Three-dimensional (3D) printing	283:313	Three-dimensional (3D) printing is an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold.
33960765	2	5	theme	innovative	321:330	arg1	method					332:337	an innovative method	318:337	an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold	318:542	Three-dimensional (3D) printing is an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold.
33960765	1	6	theme	great	230:234	arg1	importance					236:245	great importance	230:245	great importance	230:245	Biomimetic constructs imitating the functions, structures, and compositions of normal tissues are of great importance for tissue repair and regeneration.
33960765	9	7	theme	good	1800:1803	arg1	integrity					1816:1824	good structural integrity	1800:1824	good structural integrity	1800:1824	Together, the printable biomaterial inks with shear thinning and good structural integrity, along with biological cues, are promising for tissue engineering application.
33960765	6	8	theme	precise	1345:1351	arg1	deposition					1353:1362	the precise deposition	1341:1362	the precise deposition of multilayered 3D scaffolds	1341:1391	The rheological behavior of the hydrogels and the mechanical strengths of the printed constructs are tuned by adjusting the content of GelMA, which in turn enhances the shape retention after printing and enables the precise deposition of multilayered 3D scaffolds.
33960765	2	9	theme	3D	373:374	arg1	scaffolds					395:403	intricate biomimetic 3D tissue engineering scaffolds	352:403	intricate biomimetic 3D tissue engineering scaffolds	352:403	Three-dimensional (3D) printing is an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold.
33960765	5	10	theme	thermal	976:982	arg1	gelation					984:991	thermal gelation	976:991	thermal gelation for postprinting structural stability	976:1029	This polymer composite is first physically cross-linked by thermal gelation for postprinting structural stability, followed by covalent photo-cross-linking of ChMA and GelMA to form a long-term stable structure.
33960765	7	11	theme	stem	1531:1534	arg1	cells					1536:1540	stem cells	1531:1540	stem cells seeded on the scaffolds after 7 days of in vitro culture	1531:1597	Moreover, the formulated biomaterial inks exhibit biological characteristics that effectively support the spreading and proliferation of stem cells seeded on the scaffolds after 7 days of in vitro culture.
33960765	2	12	dep	Three-dimensional	283:299	arg1	3D					302:303	3D	302:303	3D	302:303	Three-dimensional (3D) printing is an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold.
33960765	3	13	theme	biological	640:649	arg1	compatibility					651:663	biological compatibility	640:663	biological compatibility	640:663	However, due to the lack of bioinks with suitable printability, high structural integrity, and biological compatibility, producing constructs that mimic the anisotropic 3D extracellular environments remains a challenge.
33960765	9	14	with	inks	1771:1774	arg1	integrity					1816:1824	good structural integrity	1800:1824	good structural integrity	1800:1824	Together, the printable biomaterial inks with shear thinning and good structural integrity, along with biological cues, are promising for tissue engineering application.
33960765	9	14	with	inks	1771:1774	arg1	thinning					1787:1794	shear thinning	1781:1794	shear thinning	1781:1794	Together, the printable biomaterial inks with shear thinning and good structural integrity, along with biological cues, are promising for tissue engineering application.
33960765	9	15	theme	biological	1838:1847	arg1	cues					1849:1852	biological cues	1838:1852	biological cues	1838:1852	Together, the printable biomaterial inks with shear thinning and good structural integrity, along with biological cues, are promising for tissue engineering application.
33960765	4	16	theme	methylacrylate-modified	816:838	arg1	ChMA					850:853	ChMA	850:853	ChMA	850:853	Here, we present a printable hydrogel ink based on methylacrylate-modified chitosan (ChMA) and gelatin (GelMA) embedding nanohydroxyapatite (nano-Hap).
33960765	4	16	theme	methylacrylate-modified	816:838	arg1	chitosan					840:847	methylacrylate-modified chitosan	816:847	methylacrylate-modified chitosan (ChMA)	816:854	Here, we present a printable hydrogel ink based on methylacrylate-modified chitosan (ChMA) and gelatin (GelMA) embedding nanohydroxyapatite (nano-Hap).
33960765	3	17	theme	extracellular	717:729	arg1	environments					731:742	the anisotropic 3D extracellular environments	698:742	the anisotropic 3D extracellular environments	698:742	However, due to the lack of bioinks with suitable printability, high structural integrity, and biological compatibility, producing constructs that mimic the anisotropic 3D extracellular environments remains a challenge.
33960765	6	18	theme	rheological	1133:1143	arg1	behavior					1145:1152	The rheological behavior	1129:1152	The rheological behavior of the hydrogels	1129:1169	The rheological behavior of the hydrogels and the mechanical strengths of the printed constructs are tuned by adjusting the content of GelMA, which in turn enhances the shape retention after printing and enables the precise deposition of multilayered 3D scaffolds.
33960765	8	19	theme	Adding	1600:1605	arg1	Hap					1607:1609	Adding Hap	1600:1609	Adding Hap	1600:1609	Adding Hap has minor influences on the mechanical rigidity and cytocompatibility of the hydrogels compared with the group free of Hap.
33960765	6	20	theme	shape	1298:1302	arg1	retention					1304:1312	the shape retention	1294:1312	the shape retention after printing	1294:1327	The rheological behavior of the hydrogels and the mechanical strengths of the printed constructs are tuned by adjusting the content of GelMA, which in turn enhances the shape retention after printing and enables the precise deposition of multilayered 3D scaffolds.
33960765	1	21	dep	functions	165:173	arg1	the					161:163	the	161:163	the	161:163	Biomimetic constructs imitating the functions, structures, and compositions of normal tissues are of great importance for tissue repair and regeneration.
33960765	3	22	theme	anisotropic	702:712	arg1	environments					731:742	the anisotropic 3D extracellular environments	698:742	the anisotropic 3D extracellular environments	698:742	However, due to the lack of bioinks with suitable printability, high structural integrity, and biological compatibility, producing constructs that mimic the anisotropic 3D extracellular environments remains a challenge.
33960765	1	23	theme	normal	208:213	arg1	tissues					215:221	normal tissues	208:221	normal tissues	208:221	Biomimetic constructs imitating the functions, structures, and compositions of normal tissues are of great importance for tissue repair and regeneration.
33960765	9	24	theme	tissue	1873:1878	arg1	engineering					1880:1890	tissue engineering	1873:1890	tissue engineering application	1873:1902	Together, the printable biomaterial inks with shear thinning and good structural integrity, along with biological cues, are promising for tissue engineering application.
33960765	4	25	theme	hydrogel	794:801	arg1	ink					803:805	a printable hydrogel ink	782:805	a printable hydrogel ink based on methylacrylate-modified chitosan (ChMA) and gelatin (GelMA) embedding nanohydroxyapatite (nano-Hap)	782:914	Here, we present a printable hydrogel ink based on methylacrylate-modified chitosan (ChMA) and gelatin (GelMA) embedding nanohydroxyapatite (nano-Hap).
33960765	5	26	theme	structural	1010:1019	arg1	stability					1021:1029	structural stability	1010:1029	structural stability	1010:1029	This polymer composite is first physically cross-linked by thermal gelation for postprinting structural stability, followed by covalent photo-cross-linking of ChMA and GelMA to form a long-term stable structure.
33960765	5	27	theme	GelMA	1085:1089	arg1	photo-cross-linking					1053:1071	covalent photo-cross-linking	1044:1071	covalent photo-cross-linking of ChMA and GelMA	1044:1089	This polymer composite is first physically cross-linked by thermal gelation for postprinting structural stability, followed by covalent photo-cross-linking of ChMA and GelMA to form a long-term stable structure.
33960765	6	28	theme	hydrogels	1161:1169	arg1	strengths					1190:1198	the mechanical strengths	1175:1198	the mechanical strengths of the printed constructs	1175:1224	The rheological behavior of the hydrogels and the mechanical strengths of the printed constructs are tuned by adjusting the content of GelMA, which in turn enhances the shape retention after printing and enables the precise deposition of multilayered 3D scaffolds.
33960765	6	28	theme	hydrogels	1161:1169	arg1	behavior					1145:1152	The rheological behavior	1129:1152	The rheological behavior of the hydrogels	1129:1169	The rheological behavior of the hydrogels and the mechanical strengths of the printed constructs are tuned by adjusting the content of GelMA, which in turn enhances the shape retention after printing and enables the precise deposition of multilayered 3D scaffolds.
33960765	2	29	theme	architectural	474:486	arg1	organization					488:499	intrinsic architectural organization	464:499	intrinsic architectural organization	464:499	Three-dimensional (3D) printing is an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold.
33960765	0	30	theme	Three-Dimensional-Printable	0:26	arg1	Composites					94:103	Three-Dimensional-Printable Thermo/Photo-Cross-Linked Methacrylated Chitosan-Gelatin Hydrogel Composites	0:103	Three-Dimensional-Printable Thermo/Photo-Cross-Linked Methacrylated Chitosan-Gelatin Hydrogel Composites for Tissue Engineering	0:126	Three-Dimensional-Printable Thermo/Photo-Cross-Linked Methacrylated Chitosan-Gelatin Hydrogel Composites for Tissue Engineering.
33960765	0	31	theme	Methacrylated	54:66	arg1	Composites					94:103	Three-Dimensional-Printable Thermo/Photo-Cross-Linked Methacrylated Chitosan-Gelatin Hydrogel Composites	0:103	Three-Dimensional-Printable Thermo/Photo-Cross-Linked Methacrylated Chitosan-Gelatin Hydrogel Composites for Tissue Engineering	0:126	Three-Dimensional-Printable Thermo/Photo-Cross-Linked Methacrylated Chitosan-Gelatin Hydrogel Composites for Tissue Engineering.
33960765	5	32	theme	ChMA	1076:1079	arg1	photo-cross-linking					1053:1071	covalent photo-cross-linking	1044:1071	covalent photo-cross-linking of ChMA and GelMA	1044:1089	This polymer composite is first physically cross-linked by thermal gelation for postprinting structural stability, followed by covalent photo-cross-linking of ChMA and GelMA to form a long-term stable structure.
33960765	7	33	theme	biomaterial	1419:1429	arg1	inks					1431:1434	the formulated biomaterial inks	1404:1434	the formulated biomaterial inks	1404:1434	Moreover, the formulated biomaterial inks exhibit biological characteristics that effectively support the spreading and proliferation of stem cells seeded on the scaffolds after 7 days of in vitro culture.
33960765	0	34	theme	Hydrogel	85:92	arg1	Composites					94:103	Three-Dimensional-Printable Thermo/Photo-Cross-Linked Methacrylated Chitosan-Gelatin Hydrogel Composites	0:103	Three-Dimensional-Printable Thermo/Photo-Cross-Linked Methacrylated Chitosan-Gelatin Hydrogel Composites for Tissue Engineering	0:126	Three-Dimensional-Printable Thermo/Photo-Cross-Linked Methacrylated Chitosan-Gelatin Hydrogel Composites for Tissue Engineering.
33960765	2	35	theme	spatiotemporal	410:423	arg1	deposition					425:434	spatiotemporal deposition	410:434	spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold	410:542	Three-dimensional (3D) printing is an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold.
33960765	7	36	dep	in	1582:1583	arg1	vitro					1585:1589	vitro	1585:1589	vitro	1585:1589	Moreover, the formulated biomaterial inks exhibit biological characteristics that effectively support the spreading and proliferation of stem cells seeded on the scaffolds after 7 days of in vitro culture.
33960765	4	37	theme	embedding	876:884	arg1	nanohydroxyapatite					886:903	gelatin (GelMA) embedding nanohydroxyapatite	860:903	gelatin (GelMA) embedding nanohydroxyapatite (nano-Hap)	860:914	Here, we present a printable hydrogel ink based on methylacrylate-modified chitosan (ChMA) and gelatin (GelMA) embedding nanohydroxyapatite (nano-Hap).
33960765	4	37	theme	embedding	876:884	arg1	nano-Hap					906:913	nano-Hap	906:913	nano-Hap	906:913	Here, we present a printable hydrogel ink based on methylacrylate-modified chitosan (ChMA) and gelatin (GelMA) embedding nanohydroxyapatite (nano-Hap).
33960765	3	38	theme	structural	614:623	arg1	integrity					625:633	high structural integrity	609:633	high structural integrity	609:633	However, due to the lack of bioinks with suitable printability, high structural integrity, and biological compatibility, producing constructs that mimic the anisotropic 3D extracellular environments remains a challenge.
33960765	5	39	theme	long-term	1101:1109	arg1	structure					1118:1126	a long-term stable structure	1099:1126	a long-term stable structure	1099:1126	This polymer composite is first physically cross-linked by thermal gelation for postprinting structural stability, followed by covalent photo-cross-linking of ChMA and GelMA to form a long-term stable structure.
33960765	8	40	theme	free	1722:1725	arg1	group					1716:1720	the group	1712:1720	the group free of Hap	1712:1732	Adding Hap has minor influences on the mechanical rigidity and cytocompatibility of the hydrogels compared with the group free of Hap.
33960765	9	41	theme	printable	1749:1757	arg1	promising					1859:1867	promising	1859:1867	promising	1859:1867	Together, the printable biomaterial inks with shear thinning and good structural integrity, along with biological cues, are promising for tissue engineering application.
33960765	9	41	theme	printable	1749:1757	arg1	inks					1771:1774	the printable biomaterial inks	1745:1774	the printable biomaterial inks with shear thinning and good structural integrity	1745:1824	Together, the printable biomaterial inks with shear thinning and good structural integrity, along with biological cues, are promising for tissue engineering application.
33960765	6	42	theme	constructs	1215:1224	arg1	strengths					1190:1198	the mechanical strengths	1175:1198	the mechanical strengths of the printed constructs	1175:1224	The rheological behavior of the hydrogels and the mechanical strengths of the printed constructs are tuned by adjusting the content of GelMA, which in turn enhances the shape retention after printing and enables the precise deposition of multilayered 3D scaffolds.
33960765	6	42	theme	constructs	1215:1224	arg1	behavior					1145:1152	The rheological behavior	1129:1152	The rheological behavior of the hydrogels	1129:1169	The rheological behavior of the hydrogels and the mechanical strengths of the printed constructs are tuned by adjusting the content of GelMA, which in turn enhances the shape retention after printing and enables the precise deposition of multilayered 3D scaffolds.
33960765	8	43	theme	Hap	1730:1732	arg1	free					1722:1725	free	1722:1725	free	1722:1725	Adding Hap has minor influences on the mechanical rigidity and cytocompatibility of the hydrogels compared with the group free of Hap.
33960765	7	44	theme	culture	1591:1597	arg1	days					1574:1577	7 days	1572:1577	7 days of in vitro culture	1572:1597	Moreover, the formulated biomaterial inks exhibit biological characteristics that effectively support the spreading and proliferation of stem cells seeded on the scaffolds after 7 days of in vitro culture.
33960765	6	45	theme	scaffolds	1383:1391	arg1	deposition					1353:1362	the precise deposition	1341:1362	the precise deposition of multilayered 3D scaffolds	1341:1391	The rheological behavior of the hydrogels and the mechanical strengths of the printed constructs are tuned by adjusting the content of GelMA, which in turn enhances the shape retention after printing and enables the precise deposition of multilayered 3D scaffolds.
33960765	7	46	theme	in	1582:1583	arg1	culture					1591:1597	in vitro culture	1582:1597	in vitro culture	1582:1597	Moreover, the formulated biomaterial inks exhibit biological characteristics that effectively support the spreading and proliferation of stem cells seeded on the scaffolds after 7 days of in vitro culture.
33960765	9	47	theme	shear	1781:1785	arg1	thinning					1787:1794	shear thinning	1781:1794	shear thinning	1781:1794	Together, the printable biomaterial inks with shear thinning and good structural integrity, along with biological cues, are promising for tissue engineering application.
33960765	6	48	theme	multilayered	1367:1378	arg1	scaffolds					1383:1391	multilayered 3D scaffolds	1367:1391	multilayered 3D scaffolds	1367:1391	The rheological behavior of the hydrogels and the mechanical strengths of the printed constructs are tuned by adjusting the content of GelMA, which in turn enhances the shape retention after printing and enables the precise deposition of multilayered 3D scaffolds.
33960765	3	49	theme	high	609:612	arg1	integrity					625:633	high structural integrity	609:633	high structural integrity	609:633	However, due to the lack of bioinks with suitable printability, high structural integrity, and biological compatibility, producing constructs that mimic the anisotropic 3D extracellular environments remains a challenge.
33960765	7	50	dep	spreading	1500:1508	arg1	the					1496:1498	the	1496:1498	the	1496:1498	Moreover, the formulated biomaterial inks exhibit biological characteristics that effectively support the spreading and proliferation of stem cells seeded on the scaffolds after 7 days of in vitro culture.
33960765	2	51	theme	tissue	376:381	arg1	scaffolds					395:403	intricate biomimetic 3D tissue engineering scaffolds	352:403	intricate biomimetic 3D tissue engineering scaffolds	352:403	Three-dimensional (3D) printing is an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold.
33960765	3	52	with	bioinks	573:579	arg1	integrity					625:633	high structural integrity	609:633	high structural integrity	609:633	However, due to the lack of bioinks with suitable printability, high structural integrity, and biological compatibility, producing constructs that mimic the anisotropic 3D extracellular environments remains a challenge.
33960765	3	52	with	bioinks	573:579	arg1	printability					595:606	suitable printability	586:606	suitable printability	586:606	However, due to the lack of bioinks with suitable printability, high structural integrity, and biological compatibility, producing constructs that mimic the anisotropic 3D extracellular environments remains a challenge.
33960765	3	52	with	bioinks	573:579	arg1	compatibility					651:663	biological compatibility	640:663	biological compatibility	640:663	However, due to the lack of bioinks with suitable printability, high structural integrity, and biological compatibility, producing constructs that mimic the anisotropic 3D extracellular environments remains a challenge.
33960765	6	53	theme	GelMA	1264:1268	arg1	content					1253:1259	the content	1249:1259	the content	1249:1259	The rheological behavior of the hydrogels and the mechanical strengths of the printed constructs are tuned by adjusting the content of GelMA, which in turn enhances the shape retention after printing and enables the precise deposition of multilayered 3D scaffolds.
33960765	2	54	theme	biomimetic	362:371	arg1	scaffolds					395:403	intricate biomimetic 3D tissue engineering scaffolds	352:403	intricate biomimetic 3D tissue engineering scaffolds	352:403	Three-dimensional (3D) printing is an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold.
33960765	5	55	theme	polymer	922:928	arg1	composite					930:938	This polymer composite	917:938	This polymer composite	917:938	This polymer composite is first physically cross-linked by thermal gelation for postprinting structural stability, followed by covalent photo-cross-linking of ChMA and GelMA to form a long-term stable structure.
33960765	7	56	theme	cells	1536:1540	arg1	proliferation					1514:1526	proliferation	1514:1526	proliferation	1514:1526	Moreover, the formulated biomaterial inks exhibit biological characteristics that effectively support the spreading and proliferation of stem cells seeded on the scaffolds after 7 days of in vitro culture.
33960765	7	56	theme	cells	1536:1540	arg1	spreading					1500:1508	spreading	1500:1508	spreading	1500:1508	Moreover, the formulated biomaterial inks exhibit biological characteristics that effectively support the spreading and proliferation of stem cells seeded on the scaffolds after 7 days of in vitro culture.
33960765	8	57	contain	has	1611:1613	arg2	influences					1621:1630	minor influences	1615:1630	minor influences	1615:1630	Adding Hap has minor influences on the mechanical rigidity and cytocompatibility of the hydrogels compared with the group free of Hap.
33960765	8	57	contain	has	1611:1613	arg1	Hap					1607:1609	Adding Hap	1600:1609	Adding Hap	1600:1609	Adding Hap has minor influences on the mechanical rigidity and cytocompatibility of the hydrogels compared with the group free of Hap.
33960765	9	58	theme	structural	1805:1814	arg1	integrity					1816:1824	good structural integrity	1800:1824	good structural integrity	1800:1824	Together, the printable biomaterial inks with shear thinning and good structural integrity, along with biological cues, are promising for tissue engineering application.
33960765	3	59	theme	producing	666:674	arg1	constructs					676:685	producing constructs	666:685	producing constructs that mimic the anisotropic 3D extracellular environments	666:742	However, due to the lack of bioinks with suitable printability, high structural integrity, and biological compatibility, producing constructs that mimic the anisotropic 3D extracellular environments remains a challenge.
33960765	3	60	theme	3D	714:715	arg1	environments					731:742	the anisotropic 3D extracellular environments	698:742	the anisotropic 3D extracellular environments	698:742	However, due to the lack of bioinks with suitable printability, high structural integrity, and biological compatibility, producing constructs that mimic the anisotropic 3D extracellular environments remains a challenge.
33960765	9	61	dep	along	1827:1831	arg1	with					1833:1836	with	1833:1836	with	1833:1836	Together, the printable biomaterial inks with shear thinning and good structural integrity, along with biological cues, are promising for tissue engineering application.
33960765	1	62	theme	tissue	251:256	arg1	repair					258:263	tissue repair	251:263	tissue repair	251:263	Biomimetic constructs imitating the functions, structures, and compositions of normal tissues are of great importance for tissue repair and regeneration.
33960765	8	63	theme	minor	1615:1619	arg1	influences					1621:1630	minor influences	1615:1630	minor influences	1615:1630	Adding Hap has minor influences on the mechanical rigidity and cytocompatibility of the hydrogels compared with the group free of Hap.
33960765	1	64	theme	tissues	215:221	arg1	structures					176:185	structures	176:185	structures	176:185	Biomimetic constructs imitating the functions, structures, and compositions of normal tissues are of great importance for tissue repair and regeneration.
33960765	1	64	theme	tissues	215:221	arg1	compositions					192:203	compositions	192:203	compositions	192:203	Biomimetic constructs imitating the functions, structures, and compositions of normal tissues are of great importance for tissue repair and regeneration.
33960765	1	64	theme	tissues	215:221	arg1	functions					165:173	functions	165:173	functions	165:173	Biomimetic constructs imitating the functions, structures, and compositions of normal tissues are of great importance for tissue repair and regeneration.
33960765	9	65	theme	engineering	1880:1890	arg1	application					1892:1902	tissue engineering application	1873:1902	tissue engineering application	1873:1902	Together, the printable biomaterial inks with shear thinning and good structural integrity, along with biological cues, are promising for tissue engineering application.
33960765	4	66	theme	printable	784:792	arg1	ink					803:805	a printable hydrogel ink	782:805	a printable hydrogel ink based on methylacrylate-modified chitosan (ChMA) and gelatin (GelMA) embedding nanohydroxyapatite (nano-Hap)	782:914	Here, we present a printable hydrogel ink based on methylacrylate-modified chitosan (ChMA) and gelatin (GelMA) embedding nanohydroxyapatite (nano-Hap).
33960765	2	67	theme	intricate	352:360	arg1	scaffolds					395:403	intricate biomimetic 3D tissue engineering scaffolds	352:403	intricate biomimetic 3D tissue engineering scaffolds	352:403	Three-dimensional (3D) printing is an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold.
33960765	8	68	theme	mechanical	1639:1648	arg1	rigidity					1650:1657	rigidity	1650:1657	rigidity	1650:1657	Adding Hap has minor influences on the mechanical rigidity and cytocompatibility of the hydrogels compared with the group free of Hap.
33960765	1	69	theme	Biomimetic	129:138	arg1	constructs					140:149	Biomimetic constructs	129:149	Biomimetic constructs imitating the functions, structures, and compositions of normal tissues	129:221	Biomimetic constructs imitating the functions, structures, and compositions of normal tissues are of great importance for tissue repair and regeneration.
33960765	7	70	theme	biological	1444:1453	arg1	characteristics					1455:1469	biological characteristics	1444:1469	biological characteristics that effectively support the spreading and proliferation of stem cells seeded on the scaffolds after 7 days of in vitro culture	1444:1597	Moreover, the formulated biomaterial inks exhibit biological characteristics that effectively support the spreading and proliferation of stem cells seeded on the scaffolds after 7 days of in vitro culture.
33960765	2	71	theme	intrinsic	464:472	arg1	organization					488:499	intrinsic architectural organization	464:499	intrinsic architectural organization	464:499	Three-dimensional (3D) printing is an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold.
33960765	0	72	theme	Thermo/Photo-Cross-Linked	28:52	arg1	Composites					94:103	Three-Dimensional-Printable Thermo/Photo-Cross-Linked Methacrylated Chitosan-Gelatin Hydrogel Composites	0:103	Three-Dimensional-Printable Thermo/Photo-Cross-Linked Methacrylated Chitosan-Gelatin Hydrogel Composites for Tissue Engineering	0:126	Three-Dimensional-Printable Thermo/Photo-Cross-Linked Methacrylated Chitosan-Gelatin Hydrogel Composites for Tissue Engineering.
33960765	5	73	theme	covalent	1044:1051	arg1	photo-cross-linking					1053:1071	covalent photo-cross-linking	1044:1071	covalent photo-cross-linking of ChMA and GelMA	1044:1089	This polymer composite is first physically cross-linked by thermal gelation for postprinting structural stability, followed by covalent photo-cross-linking of ChMA and GelMA to form a long-term stable structure.
33960765	6	74	theme	mechanical	1179:1188	arg1	strengths					1190:1198	the mechanical strengths	1175:1198	the mechanical strengths of the printed constructs	1175:1224	The rheological behavior of the hydrogels and the mechanical strengths of the printed constructs are tuned by adjusting the content of GelMA, which in turn enhances the shape retention after printing and enables the precise deposition of multilayered 3D scaffolds.
33960765	2	75	theme	scaffold	535:542	arg1	organization					488:499	intrinsic architectural organization	464:499	intrinsic architectural organization	464:499	Three-dimensional (3D) printing is an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold.
33960765	2	75	theme	scaffold	535:542	arg1	performance					516:526	functional performance	505:526	functional performance	505:526	Three-dimensional (3D) printing is an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold.
33960765	0	76	theme	Chitosan-Gelatin	68:83	arg1	Composites					94:103	Three-Dimensional-Printable Thermo/Photo-Cross-Linked Methacrylated Chitosan-Gelatin Hydrogel Composites	0:103	Three-Dimensional-Printable Thermo/Photo-Cross-Linked Methacrylated Chitosan-Gelatin Hydrogel Composites for Tissue Engineering	0:126	Three-Dimensional-Printable Thermo/Photo-Cross-Linked Methacrylated Chitosan-Gelatin Hydrogel Composites for Tissue Engineering.
33960765	7	77	theme	formulated	1408:1417	arg1	inks					1431:1434	the formulated biomaterial inks	1404:1434	the formulated biomaterial inks	1404:1434	Moreover, the formulated biomaterial inks exhibit biological characteristics that effectively support the spreading and proliferation of stem cells seeded on the scaffolds after 7 days of in vitro culture.
33960765	8	78	theme	hydrogels	1688:1696	arg1	cytocompatibility					1663:1679	cytocompatibility	1663:1679	cytocompatibility	1663:1679	Adding Hap has minor influences on the mechanical rigidity and cytocompatibility of the hydrogels compared with the group free of Hap.
33960765	8	78	theme	hydrogels	1688:1696	arg1	rigidity					1650:1657	rigidity	1650:1657	rigidity	1650:1657	Adding Hap has minor influences on the mechanical rigidity and cytocompatibility of the hydrogels compared with the group free of Hap.
33960765	2	79	theme	functional	505:514	arg1	performance					516:526	functional performance	505:526	functional performance	505:526	Three-dimensional (3D) printing is an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold.
33960765	2	80	dep	organization	488:499	arg1	the					460:462	the	460:462	the	460:462	Three-dimensional (3D) printing is an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold.
33960765	0	81	theme	Tissue	109:114	arg1	Engineering					116:126	Tissue Engineering	109:126	Tissue Engineering	109:126	Three-Dimensional-Printable Thermo/Photo-Cross-Linked Methacrylated Chitosan-Gelatin Hydrogel Composites for Tissue Engineering.
33960765	5	82	dep	cross-linked	960:971	arg1	followed					1032:1039	followed	1032:1039	followed by covalent photo-cross-linking of ChMA and GelMA to form a long-term stable structure	1032:1126	This polymer composite is first physically cross-linked by thermal gelation for postprinting structural stability, followed by covalent photo-cross-linking of ChMA and GelMA to form a long-term stable structure.
33960765	3	83	theme	bioinks	573:579	arg1	lack					565:568	the lack	561:568	the lack of bioinks with suitable printability, high structural integrity, and biological compatibility	561:663	However, due to the lack of bioinks with suitable printability, high structural integrity, and biological compatibility, producing constructs that mimic the anisotropic 3D extracellular environments remains a challenge.
33960765	2	84	theme	engineering	383:393	arg1	scaffolds					395:403	intricate biomimetic 3D tissue engineering scaffolds	352:403	intricate biomimetic 3D tissue engineering scaffolds	352:403	Three-dimensional (3D) printing is an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold.
33960765	8	85	dep	rigidity	1650:1657	arg1	the					1635:1637	the	1635:1637	the	1635:1637	Adding Hap has minor influences on the mechanical rigidity and cytocompatibility of the hydrogels compared with the group free of Hap.
33960765	5	86	theme	stable	1111:1116	arg1	structure					1118:1126	a long-term stable structure	1099:1126	a long-term stable structure	1099:1126	This polymer composite is first physically cross-linked by thermal gelation for postprinting structural stability, followed by covalent photo-cross-linking of ChMA and GelMA to form a long-term stable structure.
33960765	2	87	theme	materials	439:447	arg1	deposition					425:434	spatiotemporal deposition	410:434	spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold	410:542	Three-dimensional (3D) printing is an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold.
33647198	0	0	theme	Network	84:90	arg1	Anisotropy					92:101	Gel Network Anisotropy	80:101	Gel Network Anisotropy to Calcite Crystal Form	80:125	Forming Anisotropic Crystal Composites: Assessing the Mechanical Translation of Gel Network Anisotropy to Calcite Crystal Form.
33647198	8	1	theme	direction-specific	1563:1580	arg1	properties					1606:1615	direction-specific and emergent functional properties	1563:1615	properties	1606:1615	This work highlights the important role organic matrices can play in mechanically "molding" biominerals and provides an exciting platform for fabricating crystal composites with direction-specific and emergent functional properties.
33647198	1	2	with	composites	151:160	arg1	properties					186:195	direction-specific properties	167:195	direction-specific properties	167:195	The promise of crystal composites with direction-specific properties is an attractive prospect for diverse applications; however, synthetic strategies for realizing such composites remain elusive.
33647198	3	3	theme	drying	640:645	arg1	plane					647:651	the drying plane	636:651	the drying plane	636:651	Drying and rehydration of agarose gel films result in the affine deformation of their fibrous networks to yield fiber alignment parallel to the drying plane.
33647198	1	4	theme	composites	151:160	arg1	prospect					214:221	an attractive prospect	200:221	an attractive prospect for diverse applications	200:246	The promise of crystal composites with direction-specific properties is an attractive prospect for diverse applications; however, synthetic strategies for realizing such composites remain elusive.
33647198	1	4	theme	composites	151:160	arg1	promise					132:138	The promise	128:138	The promise of crystal composites with direction-specific properties	128:195	The promise of crystal composites with direction-specific properties is an attractive prospect for diverse applications; however, synthetic strategies for realizing such composites remain elusive.
33647198	1	5	theme	synthetic	258:266	arg1	strategies					268:277	synthetic strategies	258:277	synthetic strategies for realizing such composites	258:307	The promise of crystal composites with direction-specific properties is an attractive prospect for diverse applications; however, synthetic strategies for realizing such composites remain elusive.
33647198	0	6	theme	Gel	80:82	arg1	Anisotropy					92:101	Gel Network Anisotropy	80:101	Gel Network Anisotropy to Calcite Crystal Form	80:125	Forming Anisotropic Crystal Composites: Assessing the Mechanical Translation of Gel Network Anisotropy to Calcite Crystal Form.
33647198	8	7	theme	functional	1595:1604	arg1	properties					1606:1615	direction-specific and emergent functional properties	1563:1615	properties	1606:1615	This work highlights the important role organic matrices can play in mechanically "molding" biominerals and provides an exciting platform for fabricating crystal composites with direction-specific and emergent functional properties.
33647198	6	8	theme	gel	1133:1135	arg1	cylinders					1137:1145	uniaxially deformed agarose gel cylinders	1105:1145	uniaxially deformed agarose gel cylinders	1105:1145	Precipitation of calcite in uniaxially deformed agarose gel cylinders results in the formation of rice-grain-shaped crystals, suggesting the broad applicability of the approach.
33647198	2	9	theme	gel	371:373	arg1	networks					375:382	anisotropic agarose gel networks	351:382	anisotropic agarose gel networks	351:382	Here, we demonstrate that anisotropic agarose gel networks can mechanically "mold" calcite crystal growth, yielding anisotropically structured, single-crystal composites.
33647198	8	10	theme	emergent	1586:1593	arg1	properties					1606:1615	direction-specific and emergent functional properties	1563:1615	properties	1606:1615	This work highlights the important role organic matrices can play in mechanically "molding" biominerals and provides an exciting platform for fabricating crystal composites with direction-specific and emergent functional properties.
33647198	6	11	theme	agarose	1125:1131	arg1	cylinders					1137:1145	uniaxially deformed agarose gel cylinders	1105:1145	uniaxially deformed agarose gel cylinders	1105:1145	Precipitation of calcite in uniaxially deformed agarose gel cylinders results in the formation of rice-grain-shaped crystals, suggesting the broad applicability of the approach.
33647198	6	12	theme	approach	1245:1252	arg1	applicability					1224:1236	the broad applicability	1214:1236	the broad applicability of the approach	1214:1252	Precipitation of calcite in uniaxially deformed agarose gel cylinders results in the formation of rice-grain-shaped crystals, suggesting the broad applicability of the approach.
33647198	8	13	theme	molding	1468:1474	arg1	biominerals					1477:1487	mechanically "molding" biominerals	1454:1487	mechanically "molding" biominerals	1454:1487	This work highlights the important role organic matrices can play in mechanically "molding" biominerals and provides an exciting platform for fabricating crystal composites with direction-specific and emergent functional properties.
33647198	8	14	with	composites	1547:1556	arg1	properties					1606:1615	direction-specific and emergent functional properties	1563:1615	properties	1606:1615	This work highlights the important role organic matrices can play in mechanically "molding" biominerals and provides an exciting platform for fabricating crystal composites with direction-specific and emergent functional properties.
33647198	0	15	theme	Anisotropy	92:101	arg1	Translation					65:75	the Mechanical Translation	50:75	the Mechanical Translation of Gel Network Anisotropy to Calcite Crystal Form	50:125	Forming Anisotropic Crystal Composites: Assessing the Mechanical Translation of Gel Network Anisotropy to Calcite Crystal Form.
33647198	6	16	theme	broad	1218:1222	arg1	applicability					1224:1236	the broad applicability	1214:1236	the broad applicability of the approach	1214:1252	Precipitation of calcite in uniaxially deformed agarose gel cylinders results in the formation of rice-grain-shaped crystals, suggesting the broad applicability of the approach.
33647198	3	17	theme	films	534:538	arg1	rehydration					507:517	rehydration	507:517	rehydration	507:517	Drying and rehydration of agarose gel films result in the affine deformation of their fibrous networks to yield fiber alignment parallel to the drying plane.
33647198	3	17	theme	films	534:538	arg1	Drying					496:501	Drying	496:501	Drying	496:501	Drying and rehydration of agarose gel films result in the affine deformation of their fibrous networks to yield fiber alignment parallel to the drying plane.
33647198	0	18	theme	Crystal	114:120	arg1	Form					122:125	Calcite Crystal Form	106:125	Calcite Crystal Form	106:125	Forming Anisotropic Crystal Composites: Assessing the Mechanical Translation of Gel Network Anisotropy to Calcite Crystal Form.
33647198	3	19	theme	agarose	522:528	arg1	films					534:538	agarose gel films	522:538	agarose gel films	522:538	Drying and rehydration of agarose gel films result in the affine deformation of their fibrous networks to yield fiber alignment parallel to the drying plane.
33647198	2	20	dep	structured	457:466	arg1	single-crystal					469:482	single-crystal	469:482	single-crystal	469:482	Here, we demonstrate that anisotropic agarose gel networks can mechanically "mold" calcite crystal growth, yielding anisotropically structured, single-crystal composites.
33647198	7	21	theme	composite	1361:1369	arg1	morphologies					1371:1382	the desired crystal composite morphologies	1341:1382	the desired crystal composite morphologies	1341:1382	These results demonstrate how the anisotropy of compliant networks can translate into the desired crystal composite morphologies.
33647198	3	22	theme	affine	554:559	arg1	deformation					561:571	the affine deformation	550:571	the affine deformation of their fibrous networks to yield fiber alignment parallel to the drying plane	550:651	Drying and rehydration of agarose gel films result in the affine deformation of their fibrous networks to yield fiber alignment parallel to the drying plane.
33647198	1	23	theme	direction-specific	167:184	arg1	properties					186:195	direction-specific properties	167:195	direction-specific properties	167:195	The promise of crystal composites with direction-specific properties is an attractive prospect for diverse applications; however, synthetic strategies for realizing such composites remain elusive.
33647198	0	24	theme	Calcite	106:112	arg1	Form					122:125	Calcite Crystal Form	106:125	Calcite Crystal Form	106:125	Forming Anisotropic Crystal Composites: Assessing the Mechanical Translation of Gel Network Anisotropy to Calcite Crystal Form.
33647198	5	25	theme	linear-elastic	932:945	arg1	theory					966:971	linear-elastic fracture mechanics theory	932:971	linear-elastic fracture mechanics theory	932:971	The morphology of the disks, revealed by nanocomputed tomography imaging, evolves with time and can be described by linear-elastic fracture mechanics theory, which depends on the ratio between the length of the crystal and the elastoadhesive length of the gel.
33647198	8	26	theme	important	1410:1418	arg1	role					1420:1423	the important role organic matrices can play in mechanically "molding" biominerals	1406:1487	the important role organic matrices can play in mechanically "molding" biominerals	1406:1487	This work highlights the important role organic matrices can play in mechanically "molding" biominerals and provides an exciting platform for fabricating crystal composites with direction-specific and emergent functional properties.
33647198	6	27	theme	deformed	1116:1123	arg1	cylinders					1137:1145	uniaxially deformed agarose gel cylinders	1105:1145	uniaxially deformed agarose gel cylinders	1105:1145	Precipitation of calcite in uniaxially deformed agarose gel cylinders results in the formation of rice-grain-shaped crystals, suggesting the broad applicability of the approach.
33647198	0	28	theme	Anisotropic	8:18	arg1	Composites					28:37	Anisotropic Crystal Composites	8:37	Anisotropic Crystal Composites	8:37	Forming Anisotropic Crystal Composites: Assessing the Mechanical Translation of Gel Network Anisotropy to Calcite Crystal Form.
33647198	4	29	theme	calcium	671:677	arg1	carbonate					679:687	calcium carbonate	671:687	calcium carbonate	671:687	Precipitation of calcium carbonate within these anisotropic networks results in the formation of calcite crystal composite disks oriented parallel to the fibers.
33647198	7	30	theme	compliant	1303:1311	arg1	networks					1313:1320	compliant networks	1303:1320	compliant networks	1303:1320	These results demonstrate how the anisotropy of compliant networks can translate into the desired crystal composite morphologies.
33647198	6	31	theme	calcite	1094:1100	arg1	Precipitation					1077:1089	Precipitation	1077:1089	Precipitation of calcite in uniaxially deformed agarose gel cylinders	1077:1145	Precipitation of calcite in uniaxially deformed agarose gel cylinders results in the formation of rice-grain-shaped crystals, suggesting the broad applicability of the approach.
33647198	1	32	theme	such	293:296	arg1	composites					298:307	such composites	293:307	such composites	293:307	The promise of crystal composites with direction-specific properties is an attractive prospect for diverse applications; however, synthetic strategies for realizing such composites remain elusive.
33647198	8	33	dep	role	1420:1423	arg1	play					1446:1449	play	1446:1449	can play in mechanically "molding" biominerals	1442:1487	This work highlights the important role organic matrices can play in mechanically "molding" biominerals and provides an exciting platform for fabricating crystal composites with direction-specific and emergent functional properties.
33647198	6	34	from	Precipitation	1077:1089	arg1	cylinders					1137:1145	uniaxially deformed agarose gel cylinders	1105:1145	uniaxially deformed agarose gel cylinders	1105:1145	Precipitation of calcite in uniaxially deformed agarose gel cylinders results in the formation of rice-grain-shaped crystals, suggesting the broad applicability of the approach.
33647198	4	35	theme	disks	777:781	arg1	formation					738:746	the formation	734:746	the formation of calcite crystal composite disks oriented parallel to the fibers	734:813	Precipitation of calcium carbonate within these anisotropic networks results in the formation of calcite crystal composite disks oriented parallel to the fibers.
33647198	4	36	theme	anisotropic	702:712	arg1	networks					714:721	these anisotropic networks	696:721	these anisotropic networks	696:721	Precipitation of calcium carbonate within these anisotropic networks results in the formation of calcite crystal composite disks oriented parallel to the fibers.
33647198	2	37	theme	structured	457:466	arg1	composites					484:493	anisotropically structured, single-crystal composites	441:493	anisotropically structured, single-crystal composites	441:493	Here, we demonstrate that anisotropic agarose gel networks can mechanically "mold" calcite crystal growth, yielding anisotropically structured, single-crystal composites.
33647198	3	38	theme	fibrous	582:588	arg1	networks					590:597	their fibrous networks	576:597	their fibrous networks	576:597	Drying and rehydration of agarose gel films result in the affine deformation of their fibrous networks to yield fiber alignment parallel to the drying plane.
33647198	8	39	theme	mechanically	1454:1465	arg1	biominerals					1477:1487	mechanically "molding" biominerals	1454:1487	mechanically "molding" biominerals	1454:1487	This work highlights the important role organic matrices can play in mechanically "molding" biominerals and provides an exciting platform for fabricating crystal composites with direction-specific and emergent functional properties.
33647198	1	40	theme	attractive	203:212	arg1	prospect					214:221	an attractive prospect	200:221	an attractive prospect for diverse applications	200:246	The promise of crystal composites with direction-specific properties is an attractive prospect for diverse applications; however, synthetic strategies for realizing such composites remain elusive.
33647198	1	40	theme	attractive	203:212	arg1	promise					132:138	The promise	128:138	The promise of crystal composites with direction-specific properties	128:195	The promise of crystal composites with direction-specific properties is an attractive prospect for diverse applications; however, synthetic strategies for realizing such composites remain elusive.
33647198	2	41	theme	anisotropically	441:455	arg1	composites					484:493	anisotropically structured, single-crystal composites	441:493	anisotropically structured, single-crystal composites	441:493	Here, we demonstrate that anisotropic agarose gel networks can mechanically "mold" calcite crystal growth, yielding anisotropically structured, single-crystal composites.
33647198	8	42	theme	exciting	1505:1512	arg1	platform					1514:1521	an exciting platform	1502:1521	an exciting platform for fabricating crystal composites with direction-specific and emergent functional properties	1502:1615	This work highlights the important role organic matrices can play in mechanically "molding" biominerals and provides an exciting platform for fabricating crystal composites with direction-specific and emergent functional properties.
33647198	3	43	theme	networks	590:597	arg1	deformation					561:571	the affine deformation	550:571	the affine deformation of their fibrous networks to yield fiber alignment parallel to the drying plane	550:651	Drying and rehydration of agarose gel films result in the affine deformation of their fibrous networks to yield fiber alignment parallel to the drying plane.
33647198	6	44	theme	crystals	1193:1200	arg1	formation					1162:1170	the formation	1158:1170	the formation of rice-grain-shaped crystals	1158:1200	Precipitation of calcite in uniaxially deformed agarose gel cylinders results in the formation of rice-grain-shaped crystals, suggesting the broad applicability of the approach.
33647198	7	45	theme	desired	1345:1351	arg1	morphologies					1371:1382	the desired crystal composite morphologies	1341:1382	the desired crystal composite morphologies	1341:1382	These results demonstrate how the anisotropy of compliant networks can translate into the desired crystal composite morphologies.
33647198	3	46	theme	gel	530:532	arg1	films					534:538	agarose gel films	522:538	agarose gel films	522:538	Drying and rehydration of agarose gel films result in the affine deformation of their fibrous networks to yield fiber alignment parallel to the drying plane.
33647198	3	47	theme	parallel	624:631	arg1	alignment					614:622	fiber alignment	608:622	fiber alignment parallel to the drying plane	608:651	Drying and rehydration of agarose gel films result in the affine deformation of their fibrous networks to yield fiber alignment parallel to the drying plane.
33647198	4	48	theme	carbonate	679:687	arg1	Precipitation					654:666	Precipitation	654:666	Precipitation of calcium carbonate within these anisotropic networks	654:721	Precipitation of calcium carbonate within these anisotropic networks results in the formation of calcite crystal composite disks oriented parallel to the fibers.
33647198	2	49	theme	calcite	408:414	arg1	growth					424:429	calcite crystal growth	408:429	calcite crystal growth	408:429	Here, we demonstrate that anisotropic agarose gel networks can mechanically "mold" calcite crystal growth, yielding anisotropically structured, single-crystal composites.
33647198	5	50	theme	mechanics	956:964	arg1	theory					966:971	linear-elastic fracture mechanics theory	932:971	linear-elastic fracture mechanics theory	932:971	The morphology of the disks, revealed by nanocomputed tomography imaging, evolves with time and can be described by linear-elastic fracture mechanics theory, which depends on the ratio between the length of the crystal and the elastoadhesive length of the gel.
33647198	2	51	theme	crystal	416:422	arg1	growth					424:429	calcite crystal growth	408:429	calcite crystal growth	408:429	Here, we demonstrate that anisotropic agarose gel networks can mechanically "mold" calcite crystal growth, yielding anisotropically structured, single-crystal composites.
33647198	0	52	theme	Mechanical	54:63	arg1	Translation					65:75	the Mechanical Translation	50:75	the Mechanical Translation of Gel Network Anisotropy to Calcite Crystal Form	50:125	Forming Anisotropic Crystal Composites: Assessing the Mechanical Translation of Gel Network Anisotropy to Calcite Crystal Form.
33647198	3	53	theme	fiber	608:612	arg1	alignment					614:622	fiber alignment	608:622	fiber alignment parallel to the drying plane	608:651	Drying and rehydration of agarose gel films result in the affine deformation of their fibrous networks to yield fiber alignment parallel to the drying plane.
33647198	8	54	theme	organic	1425:1431	arg1	matrices					1433:1440	organic matrices	1425:1440	organic matrices	1425:1440	This work highlights the important role organic matrices can play in mechanically "molding" biominerals and provides an exciting platform for fabricating crystal composites with direction-specific and emergent functional properties.
33647198	2	55	theme	agarose	363:369	arg1	networks					375:382	anisotropic agarose gel networks	351:382	anisotropic agarose gel networks	351:382	Here, we demonstrate that anisotropic agarose gel networks can mechanically "mold" calcite crystal growth, yielding anisotropically structured, single-crystal composites.
33647198	5	56	theme	disks	838:842	arg1	morphology					820:829	The morphology	816:829	The morphology	816:829	The morphology of the disks, revealed by nanocomputed tomography imaging, evolves with time and can be described by linear-elastic fracture mechanics theory, which depends on the ratio between the length of the crystal and the elastoadhesive length of the gel.
33647198	8	57	theme	crystal	1539:1545	arg1	composites					1547:1556	crystal composites	1539:1556	crystal composites with direction-specific and emergent functional properties	1539:1615	This work highlights the important role organic matrices can play in mechanically "molding" biominerals and provides an exciting platform for fabricating crystal composites with direction-specific and emergent functional properties.
33647198	2	58	theme	anisotropic	351:361	arg1	networks					375:382	anisotropic agarose gel networks	351:382	anisotropic agarose gel networks	351:382	Here, we demonstrate that anisotropic agarose gel networks can mechanically "mold" calcite crystal growth, yielding anisotropically structured, single-crystal composites.
33647198	2	59	dep	"	406:406	arg1	mold					402:405	mold	402:405	mold	402:405	Here, we demonstrate that anisotropic agarose gel networks can mechanically "mold" calcite crystal growth, yielding anisotropically structured, single-crystal composites.
33647198	4	60	theme	calcite	751:757	arg1	disks					777:781	calcite crystal composite disks	751:781	calcite crystal composite disks oriented parallel to the fibers	751:813	Precipitation of calcium carbonate within these anisotropic networks results in the formation of calcite crystal composite disks oriented parallel to the fibers.
33647198	5	61	theme	gel	1072:1074	arg1	length					1058:1063	the elastoadhesive length	1039:1063	the elastoadhesive length of the gel	1039:1074	The morphology of the disks, revealed by nanocomputed tomography imaging, evolves with time and can be described by linear-elastic fracture mechanics theory, which depends on the ratio between the length of the crystal and the elastoadhesive length of the gel.
33647198	5	61	theme	gel	1072:1074	arg1	length					1013:1018	the length	1009:1018	the length of the crystal	1009:1033	The morphology of the disks, revealed by nanocomputed tomography imaging, evolves with time and can be described by linear-elastic fracture mechanics theory, which depends on the ratio between the length of the crystal and the elastoadhesive length of the gel.
33647198	5	62	theme	elastoadhesive	1043:1056	arg1	length					1058:1063	the elastoadhesive length	1039:1063	the elastoadhesive length of the gel	1039:1074	The morphology of the disks, revealed by nanocomputed tomography imaging, evolves with time and can be described by linear-elastic fracture mechanics theory, which depends on the ratio between the length of the crystal and the elastoadhesive length of the gel.
33647198	4	63	theme	composite	767:775	arg1	disks					777:781	calcite crystal composite disks	751:781	calcite crystal composite disks oriented parallel to the fibers	751:813	Precipitation of calcium carbonate within these anisotropic networks results in the formation of calcite crystal composite disks oriented parallel to the fibers.
33647198	6	64	theme	rice-grain-shaped	1175:1191	arg1	crystals					1193:1200	rice-grain-shaped crystals	1175:1200	rice-grain-shaped crystals	1175:1200	Precipitation of calcite in uniaxially deformed agarose gel cylinders results in the formation of rice-grain-shaped crystals, suggesting the broad applicability of the approach.
33647198	5	65	theme	fracture	947:954	arg1	theory					966:971	linear-elastic fracture mechanics theory	932:971	linear-elastic fracture mechanics theory	932:971	The morphology of the disks, revealed by nanocomputed tomography imaging, evolves with time and can be described by linear-elastic fracture mechanics theory, which depends on the ratio between the length of the crystal and the elastoadhesive length of the gel.
33647198	5	66	theme	nanocomputed	857:868	arg1	tomography					870:879	nanocomputed tomography	857:879	nanocomputed tomography imaging	857:887	The morphology of the disks, revealed by nanocomputed tomography imaging, evolves with time and can be described by linear-elastic fracture mechanics theory, which depends on the ratio between the length of the crystal and the elastoadhesive length of the gel.
33647198	4	67	theme	crystal	759:765	arg1	disks					777:781	calcite crystal composite disks	751:781	calcite crystal composite disks oriented parallel to the fibers	751:813	Precipitation of calcium carbonate within these anisotropic networks results in the formation of calcite crystal composite disks oriented parallel to the fibers.
33647198	7	68	theme	networks	1313:1320	arg1	anisotropy					1289:1298	the anisotropy	1285:1298	the anisotropy of compliant networks	1285:1320	These results demonstrate how the anisotropy of compliant networks can translate into the desired crystal composite morphologies.
33647198	8	69	theme	"	1475:1475	arg1	biominerals					1477:1487	mechanically "molding" biominerals	1454:1487	mechanically "molding" biominerals	1454:1487	This work highlights the important role organic matrices can play in mechanically "molding" biominerals and provides an exciting platform for fabricating crystal composites with direction-specific and emergent functional properties.
33647198	1	70	theme	diverse	227:233	arg1	applications					235:246	diverse applications	227:246	diverse applications	227:246	The promise of crystal composites with direction-specific properties is an attractive prospect for diverse applications; however, synthetic strategies for realizing such composites remain elusive.
33647198	5	71	theme	crystal	1027:1033	arg1	length					1058:1063	the elastoadhesive length	1039:1063	the elastoadhesive length of the gel	1039:1074	The morphology of the disks, revealed by nanocomputed tomography imaging, evolves with time and can be described by linear-elastic fracture mechanics theory, which depends on the ratio between the length of the crystal and the elastoadhesive length of the gel.
33647198	5	71	theme	crystal	1027:1033	arg1	length					1013:1018	the length	1009:1018	the length of the crystal	1009:1033	The morphology of the disks, revealed by nanocomputed tomography imaging, evolves with time and can be described by linear-elastic fracture mechanics theory, which depends on the ratio between the length of the crystal and the elastoadhesive length of the gel.
33647198	7	72	theme	crystal	1353:1359	arg1	morphologies					1371:1382	the desired crystal composite morphologies	1341:1382	the desired crystal composite morphologies	1341:1382	These results demonstrate how the anisotropy of compliant networks can translate into the desired crystal composite morphologies.
33647198	0	73	theme	Crystal	20:26	arg1	Composites					28:37	Anisotropic Crystal Composites	8:37	Anisotropic Crystal Composites	8:37	Forming Anisotropic Crystal Composites: Assessing the Mechanical Translation of Gel Network Anisotropy to Calcite Crystal Form.
33647198	1	74	theme	crystal	143:149	arg1	composites					151:160	crystal composites	143:160	crystal composites with direction-specific properties	143:195	The promise of crystal composites with direction-specific properties is an attractive prospect for diverse applications; however, synthetic strategies for realizing such composites remain elusive.
34899084	2	0	theme	present	359:365	arg1	study					367:371	the present study	355:371	the present study	355:371	The purpose of the present study was to develop and to characterize the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA).
34899084	9	1	theme	other	1444:1448	arg1	components					1450:1459	other components	1444:1459	other components	1444:1459	The composite was then evaluated in terms of its bioactivity using SEM/EDS analyses after immersion in SBF for 12, 24, 48, and 72 h. RESULTS FTIR analysis confirmed, among other components, the presence of Si-O-Ca and Si-O-Si bonds, compatible with bioglass.
34899084	10	2	with	composite	1556:1564	arg1	structure					1580:1588	a porous structure	1571:1588	a porous structure without spikes	1571:1603	SEM analysis exhibited a composite with a porous structure without spikes.
34899084	1	3	theme	ceramic	279:285	arg1	materials					287:295	ceramic materials	279:295	ceramic materials	279:295	OBJECTIVES Bioglass composites and polymers are materials of great interest for the medical and dental areas due to their properties, combining the bioactivity of ceramic materials and the mechanical properties of polymers.
34899084	11	4	theme	P	1666:1666	arg1	presence					1642:1649	the presence	1638:1649	the presence of Si, Ca, and P in the composite	1638:1683	The elemental mapping confirmed the presence of Si, Ca, and P in the composite.
34899084	13	5	theme	Ca	1924:1925	arg1	function					1938:1945	a function	1936:1945	a function of time	1936:1953	It has also demonstrated ionic changes in the composite surface after immersion in SBF, with increasing detection of Ca and P as a function of time, highlighting its chemical bioactivity.
34899084	13	5	theme	Ca	1924:1925	arg1	detection					1911:1919	detection	1911:1919	detection of Ca and P	1911:1931	It has also demonstrated ionic changes in the composite surface after immersion in SBF, with increasing detection of Ca and P as a function of time, highlighting its chemical bioactivity.
34899084	11	6	from	presence	1642:1649	arg1	composite					1675:1683	the composite	1671:1683	the composite	1671:1683	The elemental mapping confirmed the presence of Si, Ca, and P in the composite.
34899084	8	7	theme	composite	1240:1248	arg1	strength					1262:1269	the composite compression strength	1236:1269	the composite compression strength	1236:1269	The porosity of the composite was analyzed using µCT, which also provided the composite compression strength.
34899084	13	8	theme	P	1931:1931	arg1	function					1938:1945	a function	1936:1945	a function of time	1936:1953	It has also demonstrated ionic changes in the composite surface after immersion in SBF, with increasing detection of Ca and P as a function of time, highlighting its chemical bioactivity.
34899084	13	8	theme	P	1931:1931	arg1	detection					1911:1919	detection	1911:1919	detection of Ca and P	1911:1931	It has also demonstrated ionic changes in the composite surface after immersion in SBF, with increasing detection of Ca and P as a function of time, highlighting its chemical bioactivity.
34899084	9	9	theme	Si-O-Ca	1478:1484	arg1	presence					1466:1473	the presence	1462:1473	the presence of Si-O-Ca and Si-O-Si bonds	1462:1502	The composite was then evaluated in terms of its bioactivity using SEM/EDS analyses after immersion in SBF for 12, 24, 48, and 72 h. RESULTS FTIR analysis confirmed, among other components, the presence of Si-O-Ca and Si-O-Si bonds, compatible with bioglass.
34899084	14	10	theme	resistant	2169:2177	arg1	structure					2145:2153	a three-dimensional, well-structured, chemically bioactive porous structure	2079:2153	a three-dimensional, well-structured, chemically bioactive porous structure	2079:2153	CONCLUSIONS It can be concluded that the proposed bioglass-based composite presents a three-dimensional, well-structured, chemically bioactive porous structure, mechanically resistant for being reinforced with polymeric phases, with promising results as a synthetic bone graft, which makes it suitable for guided bone regeneration.
34899084	14	11	dep	CONCLUSIONS	1995:2005	arg1	concluded					2017:2025	concluded	2017:2025	can be concluded that the proposed bioglass-based composite presents a three-dimensional, well-structured, chemically bioactive porous structure, mechanically resistant for being reinforced with polymeric phases, with promising results as a synthetic bone graft, which makes it suitable for guided bone regeneration	2010:2324	CONCLUSIONS It can be concluded that the proposed bioglass-based composite presents a three-dimensional, well-structured, chemically bioactive porous structure, mechanically resistant for being reinforced with polymeric phases, with promising results as a synthetic bone graft, which makes it suitable for guided bone regeneration.
34899084	9	12	theme	Si-O-Si	1490:1496	arg1	bonds					1498:1502	Si-O-Si bonds	1490:1502	Si-O-Si bonds	1490:1502	The composite was then evaluated in terms of its bioactivity using SEM/EDS analyses after immersion in SBF for 12, 24, 48, and 72 h. RESULTS FTIR analysis confirmed, among other components, the presence of Si-O-Ca and Si-O-Si bonds, compatible with bioglass.
34899084	3	13	with	compatibility	603:615	arg1	bioglass					643:650	bioglass	643:650	bioglass	643:650	The compatibility of functional groups with bioglass was previously evaluated.
34899084	4	14	theme	composition	761:771	arg1	terms					730:734	terms	730:734	terms of morphology, elemental composition, compressive strength, porosity, and bioactivity	730:820	The composite was then synthesized and evaluated in terms of morphology, elemental composition, compressive strength, porosity, and bioactivity.
34899084	14	15	theme	porous	2138:2143	arg1	structure					2145:2153	a three-dimensional, well-structured, chemically bioactive porous structure	2079:2153	a three-dimensional, well-structured, chemically bioactive porous structure	2079:2153	CONCLUSIONS It can be concluded that the proposed bioglass-based composite presents a three-dimensional, well-structured, chemically bioactive porous structure, mechanically resistant for being reinforced with polymeric phases, with promising results as a synthetic bone graft, which makes it suitable for guided bone regeneration.
34899084	3	16	theme	groups	631:636	arg1	compatibility					603:615	The compatibility	599:615	The compatibility of functional groups with bioglass	599:650	The compatibility of functional groups with bioglass was previously evaluated.
34899084	12	17	theme	porous	1714:1719	arg1	structure					1721:1729	a porous structure	1712:1729	a porous structure with 42.67% of open pores	1712:1755	µCT analysis demonstrated a porous structure with 42.67% of open pores and an average compression strength of 124.7 MPa.
34899084	6	18	theme	graft	989:993	arg1	composite					995:1003	The bone graft composite	980:1003	The bone graft composite	980:1003	The bone graft composite was then synthesized associating the bioglass with PVA, surfactant Triton X, and Na-CMC.
34899084	12	19	theme	pores	1751:1755	arg1	%					1741:1741	42.67%	1736:1741	42.67% of open pores	1736:1755	µCT analysis demonstrated a porous structure with 42.67% of open pores and an average compression strength of 124.7 MPa.
34899084	12	19	theme	pores	1751:1755	arg1	pores					1751:1755	open pores	1746:1755	open pores	1746:1755	µCT analysis demonstrated a porous structure with 42.67% of open pores and an average compression strength of 124.7 MPa.
34899084	4	20	theme	compressive	774:784	arg1	strength					786:793	compressive strength	774:793	compressive strength	774:793	The composite was then synthesized and evaluated in terms of morphology, elemental composition, compressive strength, porosity, and bioactivity.
34899084	9	21	theme	bioactivity	1321:1331	arg1	terms					1308:1312	terms	1308:1312	terms of its bioactivity using SEM/EDS analyses after immersion in SBF for 12, 24, 48, and 72 h. RESULTS FTIR analysis confirmed, among other components, the presence of Si-O-Ca and Si-O-Si bonds, compatible with bioglass	1308:1528	The composite was then evaluated in terms of its bioactivity using SEM/EDS analyses after immersion in SBF for 12, 24, 48, and 72 h. RESULTS FTIR analysis confirmed, among other components, the presence of Si-O-Ca and Si-O-Si bonds, compatible with bioglass.
34899084	14	22	theme	bone	2261:2264	arg1	graft					2266:2270	a synthetic bone graft	2249:2270	a synthetic bone graft	2249:2270	CONCLUSIONS It can be concluded that the proposed bioglass-based composite presents a three-dimensional, well-structured, chemically bioactive porous structure, mechanically resistant for being reinforced with polymeric phases, with promising results as a synthetic bone graft, which makes it suitable for guided bone regeneration.
34899084	1	23	dep	OBJECTIVES	116:125	arg1	polymers					151:158	polymers	151:158	polymers	151:158	OBJECTIVES Bioglass composites and polymers are materials of great interest for the medical and dental areas due to their properties, combining the bioactivity of ceramic materials and the mechanical properties of polymers.
34899084	1	23	dep	OBJECTIVES	116:125	arg1	composites					136:145	Bioglass composites	127:145	Bioglass composites	127:145	OBJECTIVES Bioglass composites and polymers are materials of great interest for the medical and dental areas due to their properties, combining the bioactivity of ceramic materials and the mechanical properties of polymers.
34899084	0	24	theme	Composite	76:84	arg1	Synthesis					9:17	Synthesis	9:17	Synthesis	9:17	Modified Synthesis and Physicochemical Characterization of a Bioglass-Based Composite for Guided Bone Regeneration.
34899084	0	24	theme	Composite	76:84	arg1	Characterization					39:54	Physicochemical Characterization	23:54	Physicochemical Characterization	23:54	Modified Synthesis and Physicochemical Characterization of a Bioglass-Based Composite for Guided Bone Regeneration.
34899084	9	25	theme	SEM/EDS	1339:1345	arg1	analyses					1347:1354	SEM/EDS analyses	1339:1354	SEM/EDS analyses	1339:1354	The composite was then evaluated in terms of its bioactivity using SEM/EDS analyses after immersion in SBF for 12, 24, 48, and 72 h. RESULTS FTIR analysis confirmed, among other components, the presence of Si-O-Ca and Si-O-Si bonds, compatible with bioglass.
34899084	12	26	theme	µCT	1686:1688	arg1	analysis					1690:1697	µCT analysis	1686:1697	µCT analysis	1686:1697	µCT analysis demonstrated a porous structure with 42.67% of open pores and an average compression strength of 124.7 MPa.
34899084	6	27	theme	Triton	1072:1077	arg1	X					1079:1079	surfactant Triton X	1061:1079	surfactant Triton X	1061:1079	The bone graft composite was then synthesized associating the bioglass with PVA, surfactant Triton X, and Na-CMC.
34899084	2	28	theme	sodium	531:536	arg1	carboxymethylcellulose					538:559	sodium carboxymethylcellulose	531:559	sodium carboxymethylcellulose (Na-CMC)	531:568	The purpose of the present study was to develop and to characterize the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA).
34899084	2	28	theme	sodium	531:536	arg1	Na-CMC					562:567	Na-CMC	562:567	Na-CMC	562:567	The purpose of the present study was to develop and to characterize the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA).
34899084	14	29	theme	guided	2301:2306	arg1	regeneration					2313:2324	guided bone regeneration	2301:2324	guided bone regeneration	2301:2324	CONCLUSIONS It can be concluded that the proposed bioglass-based composite presents a three-dimensional, well-structured, chemically bioactive porous structure, mechanically resistant for being reinforced with polymeric phases, with promising results as a synthetic bone graft, which makes it suitable for guided bone regeneration.
34899084	0	30	theme	Guided	90:95	arg1	Regeneration					102:113	Guided Bone Regeneration	90:113	Guided Bone Regeneration	90:113	Modified Synthesis and Physicochemical Characterization of a Bioglass-Based Composite for Guided Bone Regeneration.
34899084	1	31	theme	interest	183:190	arg1	OBJECTIVES					116:125	OBJECTIVES Bioglass composites and polymers	116:158	OBJECTIVES Bioglass composites and polymers	116:158	OBJECTIVES Bioglass composites and polymers are materials of great interest for the medical and dental areas due to their properties, combining the bioactivity of ceramic materials and the mechanical properties of polymers.
34899084	1	31	theme	interest	183:190	arg1	materials					164:172	materials	164:172	materials of great interest	164:190	OBJECTIVES Bioglass composites and polymers are materials of great interest for the medical and dental areas due to their properties, combining the bioactivity of ceramic materials and the mechanical properties of polymers.
34899084	2	32	theme	polyvinyl	574:582	arg1	PVA					593:595	PVA	593:595	PVA	593:595	The purpose of the present study was to develop and to characterize the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA).
34899084	2	32	theme	polyvinyl	574:582	arg1	alcohol					584:590	polyvinyl alcohol	574:590	polyvinyl alcohol (PVA)	574:596	The purpose of the present study was to develop and to characterize the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA).
34899084	4	33	theme	bioactivity	810:820	arg1	terms					730:734	terms	730:734	terms of morphology, elemental composition, compressive strength, porosity, and bioactivity	730:820	The composite was then synthesized and evaluated in terms of morphology, elemental composition, compressive strength, porosity, and bioactivity.
34899084	2	34	dep	composed	506:513	arg1	composite					496:504	an experimental bioglass-based ternary composite	457:504	the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA)	408:596	The purpose of the present study was to develop and to characterize the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA).
34899084	2	34	dep	composed	506:513	arg1	associated					515:524	associated	515:524	associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA)	515:596	The purpose of the present study was to develop and to characterize the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA).
34899084	12	35	with	structure	1721:1729	arg1	%					1741:1741	42.67%	1736:1741	42.67% of open pores	1736:1755	µCT analysis demonstrated a porous structure with 42.67% of open pores and an average compression strength of 124.7 MPa.
34899084	12	35	with	structure	1721:1729	arg1	pores					1751:1755	open pores	1746:1755	open pores	1746:1755	µCT analysis demonstrated a porous structure with 42.67% of open pores and an average compression strength of 124.7 MPa.
34899084	9	36	dep	RESULTS	1405:1411	arg1	confirmed					1427:1435	confirmed	1427:1435	confirmed	1427:1435	The composite was then evaluated in terms of its bioactivity using SEM/EDS analyses after immersion in SBF for 12, 24, 48, and 72 h. RESULTS FTIR analysis confirmed, among other components, the presence of Si-O-Ca and Si-O-Si bonds, compatible with bioglass.
34899084	13	37	theme	composite	1853:1861	arg1	surface					1863:1869	the composite surface	1849:1869	the composite surface after immersion	1849:1885	It has also demonstrated ionic changes in the composite surface after immersion in SBF, with increasing detection of Ca and P as a function of time, highlighting its chemical bioactivity.
34899084	2	38	dep	properties	446:455	arg1	composite					496:504	an experimental bioglass-based ternary composite	457:504	the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA)	408:596	The purpose of the present study was to develop and to characterize the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA).
34899084	2	38	dep	properties	446:455	arg1	associated					515:524	associated	515:524	associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA)	515:596	The purpose of the present study was to develop and to characterize the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA).
34899084	2	39	theme	bioglass-based	473:486	arg1	composite					496:504	an experimental bioglass-based ternary composite	457:504	the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA)	408:596	The purpose of the present study was to develop and to characterize the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA).
34899084	2	39	theme	bioglass-based	473:486	arg1	associated					515:524	associated	515:524	associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA)	515:596	The purpose of the present study was to develop and to characterize the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA).
34899084	9	40	with	compatible	1505:1514	arg1	bioglass					1521:1528	bioglass	1521:1528	bioglass	1521:1528	The composite was then evaluated in terms of its bioactivity using SEM/EDS analyses after immersion in SBF for 12, 24, 48, and 72 h. RESULTS FTIR analysis confirmed, among other components, the presence of Si-O-Ca and Si-O-Si bonds, compatible with bioglass.
34899084	1	41	theme	materials	287:295	arg1	bioactivity					264:274	the bioactivity	260:274	the bioactivity of ceramic materials	260:295	OBJECTIVES Bioglass composites and polymers are materials of great interest for the medical and dental areas due to their properties, combining the bioactivity of ceramic materials and the mechanical properties of polymers.
34899084	1	41	theme	materials	287:295	arg1	properties					316:325	the mechanical properties	301:325	the mechanical properties of polymers	301:337	OBJECTIVES Bioglass composites and polymers are materials of great interest for the medical and dental areas due to their properties, combining the bioactivity of ceramic materials and the mechanical properties of polymers.
34899084	13	42	theme	ionic	1832:1836	arg1	changes					1838:1844	ionic changes	1832:1844	ionic changes in the composite surface after immersion in SBF	1832:1892	It has also demonstrated ionic changes in the composite surface after immersion in SBF, with increasing detection of Ca and P as a function of time, highlighting its chemical bioactivity.
34899084	2	43	theme	morphological	432:444	arg1	properties					446:455	the physicochemical and morphological properties	408:455	the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA)	408:596	The purpose of the present study was to develop and to characterize the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA).
34899084	1	44	theme	Bioglass	127:134	arg1	composites					136:145	Bioglass composites	127:145	Bioglass composites	127:145	OBJECTIVES Bioglass composites and polymers are materials of great interest for the medical and dental areas due to their properties, combining the bioactivity of ceramic materials and the mechanical properties of polymers.
34899084	14	45	theme	proposed	2036:2043	arg1	composite					2060:2068	the proposed bioglass-based composite	2032:2068	the proposed bioglass-based composite	2032:2068	CONCLUSIONS It can be concluded that the proposed bioglass-based composite presents a three-dimensional, well-structured, chemically bioactive porous structure, mechanically resistant for being reinforced with polymeric phases, with promising results as a synthetic bone graft, which makes it suitable for guided bone regeneration.
34899084	2	46	theme	physicochemical	412:426	arg1	properties					446:455	the physicochemical and morphological properties	408:455	the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA)	408:596	The purpose of the present study was to develop and to characterize the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA).
34899084	1	47	theme	polymers	330:337	arg1	bioactivity					264:274	the bioactivity	260:274	the bioactivity of ceramic materials	260:295	OBJECTIVES Bioglass composites and polymers are materials of great interest for the medical and dental areas due to their properties, combining the bioactivity of ceramic materials and the mechanical properties of polymers.
34899084	1	47	theme	polymers	330:337	arg1	properties					316:325	the mechanical properties	301:325	the mechanical properties of polymers	301:337	OBJECTIVES Bioglass composites and polymers are materials of great interest for the medical and dental areas due to their properties, combining the bioactivity of ceramic materials and the mechanical properties of polymers.
34899084	11	48	theme	elemental	1610:1618	arg1	mapping					1620:1626	The elemental mapping	1606:1626	The elemental mapping	1606:1626	The elemental mapping confirmed the presence of Si, Ca, and P in the composite.
34899084	9	49	theme	FTIR	1413:1416	arg1	analysis					1418:1425	FTIR analysis	1413:1425	FTIR analysis	1413:1425	The composite was then evaluated in terms of its bioactivity using SEM/EDS analyses after immersion in SBF for 12, 24, 48, and 72 h. RESULTS FTIR analysis confirmed, among other components, the presence of Si-O-Ca and Si-O-Si bonds, compatible with bioglass.
34899084	10	50	theme	porous	1573:1578	arg1	structure					1580:1588	a porous structure	1571:1588	a porous structure without spikes	1571:1603	SEM analysis exhibited a composite with a porous structure without spikes.
34899084	11	51	theme	Si	1654:1655	arg1	presence					1642:1649	the presence	1638:1649	the presence of Si, Ca, and P in the composite	1638:1683	The elemental mapping confirmed the presence of Si, Ca, and P in the composite.
34899084	6	52	theme	bone	984:987	arg1	composite					995:1003	The bone graft composite	980:1003	The bone graft composite	980:1003	The bone graft composite was then synthesized associating the bioglass with PVA, surfactant Triton X, and Na-CMC.
34899084	12	53	theme	124.7 MPa	1796:1804	arg1	structure					1721:1729	a porous structure	1712:1729	a porous structure with 42.67% of open pores	1712:1755	µCT analysis demonstrated a porous structure with 42.67% of open pores and an average compression strength of 124.7 MPa.
34899084	12	53	theme	124.7 MPa	1796:1804	arg1	strength					1784:1791	an average compression strength	1761:1791	an average compression strength of 124.7 MPa	1761:1804	µCT analysis demonstrated a porous structure with 42.67% of open pores and an average compression strength of 124.7 MPa.
34899084	14	54	theme	promising	2228:2236	arg1	results					2238:2244	promising results	2228:2244	promising results as a synthetic bone graft, which makes it suitable for guided bone regeneration	2228:2324	CONCLUSIONS It can be concluded that the proposed bioglass-based composite presents a three-dimensional, well-structured, chemically bioactive porous structure, mechanically resistant for being reinforced with polymeric phases, with promising results as a synthetic bone graft, which makes it suitable for guided bone regeneration.
34899084	13	55	theme	chemical	1973:1980	arg1	bioactivity					1982:1992	its chemical bioactivity	1969:1992	its chemical bioactivity	1969:1992	It has also demonstrated ionic changes in the composite surface after immersion in SBF, with increasing detection of Ca and P as a function of time, highlighting its chemical bioactivity.
34899084	2	56	theme	study	367:371	arg1	purpose					344:350	The purpose	340:350	The purpose of the present study	340:371	The purpose of the present study was to develop and to characterize the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA).
34899084	11	57	theme	Ca	1658:1659	arg1	presence					1642:1649	the presence	1638:1649	the presence of Si, Ca, and P in the composite	1638:1683	The elemental mapping confirmed the presence of Si, Ca, and P in the composite.
34899084	14	58	dep	three-dimensional	2081:2097	arg1	well-structured					2100:2114	well-structured	2100:2114	well-structured	2100:2114	CONCLUSIONS It can be concluded that the proposed bioglass-based composite presents a three-dimensional, well-structured, chemically bioactive porous structure, mechanically resistant for being reinforced with polymeric phases, with promising results as a synthetic bone graft, which makes it suitable for guided bone regeneration.
34899084	14	58	dep	three-dimensional	2081:2097	arg1	bioactive					2128:2136	bioactive	2128:2136	bioactive	2128:2136	CONCLUSIONS It can be concluded that the proposed bioglass-based composite presents a three-dimensional, well-structured, chemically bioactive porous structure, mechanically resistant for being reinforced with polymeric phases, with promising results as a synthetic bone graft, which makes it suitable for guided bone regeneration.
34899084	12	59	theme	average	1764:1770	arg1	strength					1784:1791	an average compression strength	1761:1791	an average compression strength of 124.7 MPa	1761:1804	µCT analysis demonstrated a porous structure with 42.67% of open pores and an average compression strength of 124.7 MPa.
34899084	14	60	theme	polymeric	2205:2213	arg1	phases					2215:2220	polymeric phases	2205:2220	polymeric phases	2205:2220	CONCLUSIONS It can be concluded that the proposed bioglass-based composite presents a three-dimensional, well-structured, chemically bioactive porous structure, mechanically resistant for being reinforced with polymeric phases, with promising results as a synthetic bone graft, which makes it suitable for guided bone regeneration.
34899084	13	61	theme	time	1950:1953	arg1	function					1938:1945	a function	1936:1945	a function of time	1936:1953	It has also demonstrated ionic changes in the composite surface after immersion in SBF, with increasing detection of Ca and P as a function of time, highlighting its chemical bioactivity.
34899084	13	61	theme	time	1950:1953	arg1	detection					1911:1919	detection	1911:1919	detection of Ca and P	1911:1931	It has also demonstrated ionic changes in the composite surface after immersion in SBF, with increasing detection of Ca and P as a function of time, highlighting its chemical bioactivity.
34899084	1	62	theme	medical	200:206	arg1	areas					219:223	the medical and dental areas	196:223	the medical and dental areas due to their properties	196:247	OBJECTIVES Bioglass composites and polymers are materials of great interest for the medical and dental areas due to their properties, combining the bioactivity of ceramic materials and the mechanical properties of polymers.
34899084	5	63	theme	FTIR	931:934	arg1	analysis					936:943	FTIR analysis	931:943	FTIR analysis	931:943	MATERIALS AND METHODS The bioglass was previously synthesized using a sol-gel route and characterized using FTIR analysis to identify the functional groups.
34899084	12	64	theme	open	1746:1749	arg1	pores					1751:1755	open pores	1746:1755	open pores	1746:1755	µCT analysis demonstrated a porous structure with 42.67% of open pores and an average compression strength of 124.7 MPa.
34899084	1	65	theme	dental	212:217	arg1	areas					219:223	the medical and dental areas	196:223	the medical and dental areas due to their properties	196:247	OBJECTIVES Bioglass composites and polymers are materials of great interest for the medical and dental areas due to their properties, combining the bioactivity of ceramic materials and the mechanical properties of polymers.
34899084	8	66	theme	compression	1250:1260	arg1	strength					1262:1269	the composite compression strength	1236:1269	the composite compression strength	1236:1269	The porosity of the composite was analyzed using µCT, which also provided the composite compression strength.
34899084	5	67	dep	MATERIALS	823:831	arg1	synthesized					873:883	synthesized	873:883	synthesized using a sol-gel route	873:905	MATERIALS AND METHODS The bioglass was previously synthesized using a sol-gel route and characterized using FTIR analysis to identify the functional groups.
34899084	5	67	dep	MATERIALS	823:831	arg1	characterized					911:923	characterized	911:923	characterized using FTIR analysis	911:943	MATERIALS AND METHODS The bioglass was previously synthesized using a sol-gel route and characterized using FTIR analysis to identify the functional groups.
34899084	5	68	theme	functional	961:970	arg1	groups					972:977	the functional groups	957:977	the functional groups	957:977	MATERIALS AND METHODS The bioglass was previously synthesized using a sol-gel route and characterized using FTIR analysis to identify the functional groups.
34899084	12	69	with	strength	1784:1791	arg1	%					1741:1741	42.67%	1736:1741	42.67% of open pores	1736:1755	µCT analysis demonstrated a porous structure with 42.67% of open pores and an average compression strength of 124.7 MPa.
34899084	12	69	with	strength	1784:1791	arg1	pores					1751:1755	open pores	1746:1755	open pores	1746:1755	µCT analysis demonstrated a porous structure with 42.67% of open pores and an average compression strength of 124.7 MPa.
34899084	9	70	from	immersion	1362:1370	arg1	SBF					1375:1377	SBF	1375:1377	SBF for 12, 24, 48, and 72 h. RESULTS FTIR analysis confirmed, among other components, the presence of Si-O-Ca and Si-O-Si bonds, compatible with bioglass	1375:1528	The composite was then evaluated in terms of its bioactivity using SEM/EDS analyses after immersion in SBF for 12, 24, 48, and 72 h. RESULTS FTIR analysis confirmed, among other components, the presence of Si-O-Ca and Si-O-Si bonds, compatible with bioglass.
34899084	3	71	theme	functional	620:629	arg1	groups					631:636	functional groups	620:636	functional groups	620:636	The compatibility of functional groups with bioglass was previously evaluated.
34899084	4	72	theme	elemental	751:759	arg1	composition					761:771	elemental composition	751:771	elemental composition	751:771	The composite was then synthesized and evaluated in terms of morphology, elemental composition, compressive strength, porosity, and bioactivity.
34899084	14	73	theme	bone	2308:2311	arg1	regeneration					2313:2324	guided bone regeneration	2301:2324	guided bone regeneration	2301:2324	CONCLUSIONS It can be concluded that the proposed bioglass-based composite presents a three-dimensional, well-structured, chemically bioactive porous structure, mechanically resistant for being reinforced with polymeric phases, with promising results as a synthetic bone graft, which makes it suitable for guided bone regeneration.
34899084	0	74	theme	Bone	97:100	arg1	Regeneration					102:113	Guided Bone Regeneration	90:113	Guided Bone Regeneration	90:113	Modified Synthesis and Physicochemical Characterization of a Bioglass-Based Composite for Guided Bone Regeneration.
34899084	1	75	theme	great	177:181	arg1	interest					183:190	great interest	177:190	great interest	177:190	OBJECTIVES Bioglass composites and polymers are materials of great interest for the medical and dental areas due to their properties, combining the bioactivity of ceramic materials and the mechanical properties of polymers.
34899084	6	76	theme	surfactant	1061:1070	arg1	X					1079:1079	surfactant Triton X	1061:1079	surfactant Triton X	1061:1079	The bone graft composite was then synthesized associating the bioglass with PVA, surfactant Triton X, and Na-CMC.
34899084	4	77	theme	morphology	739:748	arg1	terms					730:734	terms	730:734	terms of morphology, elemental composition, compressive strength, porosity, and bioactivity	730:820	The composite was then synthesized and evaluated in terms of morphology, elemental composition, compressive strength, porosity, and bioactivity.
34899084	5	78	theme	sol-gel	893:899	arg1	route					901:905	a sol-gel route	891:905	a sol-gel route	891:905	MATERIALS AND METHODS The bioglass was previously synthesized using a sol-gel route and characterized using FTIR analysis to identify the functional groups.
34899084	14	79	theme	three-dimensional	2081:2097	arg1	structure					2145:2153	a three-dimensional, well-structured, chemically bioactive porous structure	2079:2153	a three-dimensional, well-structured, chemically bioactive porous structure	2079:2153	CONCLUSIONS It can be concluded that the proposed bioglass-based composite presents a three-dimensional, well-structured, chemically bioactive porous structure, mechanically resistant for being reinforced with polymeric phases, with promising results as a synthetic bone graft, which makes it suitable for guided bone regeneration.
34899084	1	80	theme	due	225:227	arg1	areas					219:223	the medical and dental areas	196:223	the medical and dental areas due to their properties	196:247	OBJECTIVES Bioglass composites and polymers are materials of great interest for the medical and dental areas due to their properties, combining the bioactivity of ceramic materials and the mechanical properties of polymers.
34899084	2	81	theme	experimental	460:471	arg1	composite					496:504	an experimental bioglass-based ternary composite	457:504	the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA)	408:596	The purpose of the present study was to develop and to characterize the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA).
34899084	2	81	theme	experimental	460:471	arg1	associated					515:524	associated	515:524	associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA)	515:596	The purpose of the present study was to develop and to characterize the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA).
34899084	4	82	theme	strength	786:793	arg1	terms					730:734	terms	730:734	terms of morphology, elemental composition, compressive strength, porosity, and bioactivity	730:820	The composite was then synthesized and evaluated in terms of morphology, elemental composition, compressive strength, porosity, and bioactivity.
34899084	0	83	theme	Physicochemical	23:37	arg1	Characterization					39:54	Physicochemical Characterization	23:54	Physicochemical Characterization	23:54	Modified Synthesis and Physicochemical Characterization of a Bioglass-Based Composite for Guided Bone Regeneration.
34899084	14	84	theme	bioglass-based	2045:2058	arg1	composite					2060:2068	the proposed bioglass-based composite	2032:2068	the proposed bioglass-based composite	2032:2068	CONCLUSIONS It can be concluded that the proposed bioglass-based composite presents a three-dimensional, well-structured, chemically bioactive porous structure, mechanically resistant for being reinforced with polymeric phases, with promising results as a synthetic bone graft, which makes it suitable for guided bone regeneration.
34899084	4	85	theme	porosity	796:803	arg1	terms					730:734	terms	730:734	terms of morphology, elemental composition, compressive strength, porosity, and bioactivity	730:820	The composite was then synthesized and evaluated in terms of morphology, elemental composition, compressive strength, porosity, and bioactivity.
34899084	14	86	theme	synthetic	2251:2259	arg1	graft					2266:2270	a synthetic bone graft	2249:2270	a synthetic bone graft	2249:2270	CONCLUSIONS It can be concluded that the proposed bioglass-based composite presents a three-dimensional, well-structured, chemically bioactive porous structure, mechanically resistant for being reinforced with polymeric phases, with promising results as a synthetic bone graft, which makes it suitable for guided bone regeneration.
34899084	1	87	theme	mechanical	305:314	arg1	properties					316:325	the mechanical properties	301:325	the mechanical properties of polymers	301:337	OBJECTIVES Bioglass composites and polymers are materials of great interest for the medical and dental areas due to their properties, combining the bioactivity of ceramic materials and the mechanical properties of polymers.
34899084	9	88	theme	bonds	1498:1502	arg1	presence					1466:1473	the presence	1462:1473	the presence of Si-O-Ca and Si-O-Si bonds	1462:1502	The composite was then evaluated in terms of its bioactivity using SEM/EDS analyses after immersion in SBF for 12, 24, 48, and 72 h. RESULTS FTIR analysis confirmed, among other components, the presence of Si-O-Ca and Si-O-Si bonds, compatible with bioglass.
34899084	13	89	from	changes	1838:1844	arg1	SBF					1890:1892	SBF	1890:1892	SBF	1890:1892	It has also demonstrated ionic changes in the composite surface after immersion in SBF, with increasing detection of Ca and P as a function of time, highlighting its chemical bioactivity.
34899084	13	89	from	changes	1838:1844	arg1	surface					1863:1869	the composite surface	1849:1869	the composite surface after immersion	1849:1885	It has also demonstrated ionic changes in the composite surface after immersion in SBF, with increasing detection of Ca and P as a function of time, highlighting its chemical bioactivity.
34899084	0	90	theme	Bioglass-Based	61:74	arg1	Composite					76:84	a Bioglass-Based Composite	59:84	a Bioglass-Based Composite for Guided Bone Regeneration	59:113	Modified Synthesis and Physicochemical Characterization of a Bioglass-Based Composite for Guided Bone Regeneration.
34899084	2	91	theme	ternary	488:494	arg1	composite					496:504	an experimental bioglass-based ternary composite	457:504	the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA)	408:596	The purpose of the present study was to develop and to characterize the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA).
34899084	2	91	theme	ternary	488:494	arg1	associated					515:524	associated	515:524	associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA)	515:596	The purpose of the present study was to develop and to characterize the physicochemical and morphological properties an experimental bioglass-based ternary composite composed associated with sodium carboxymethylcellulose (Na-CMC) and polyvinyl alcohol (PVA).
34899084	8	92	theme	composite	1182:1190	arg1	porosity					1166:1173	The porosity	1162:1173	The porosity of the composite	1162:1190	The porosity of the composite was analyzed using µCT, which also provided the composite compression strength.
34899084	10	93	theme	SEM	1531:1533	arg1	analysis					1535:1542	SEM analysis	1531:1542	SEM analysis	1531:1542	SEM analysis exhibited a composite with a porous structure without spikes.
34899084	12	94	theme	compression	1772:1782	arg1	strength					1784:1791	an average compression strength	1761:1791	an average compression strength of 124.7 MPa	1761:1804	µCT analysis demonstrated a porous structure with 42.67% of open pores and an average compression strength of 124.7 MPa.
34899084	11	95	attach	presence	1642:1649	arg2	Si					1654:1655	Si	1654:1655	Si	1654:1655	The elemental mapping confirmed the presence of Si, Ca, and P in the composite.
34899084	11	95	attach	presence	1642:1649	arg2	P					1666:1666	P	1666:1666	P	1666:1666	The elemental mapping confirmed the presence of Si, Ca, and P in the composite.
34899084	11	95	attach	presence	1642:1649	arg2	Ca					1658:1659	Ca	1658:1659	Ca	1658:1659	The elemental mapping confirmed the presence of Si, Ca, and P in the composite.
34899084	11	95	attach	presence	1642:1649	arg1	composite					1675:1683	the composite	1671:1683	the composite	1671:1683	The elemental mapping confirmed the presence of Si, Ca, and P in the composite.
33202270	0	0	theme	intestine	91:99	arg1	activity					121:128	intestine microbial metabolic activity	91:128	intestine microbial metabolic activity	91:128	Flammulina velutipes polysaccharide improves C57BL/6 mice gut health through regulation of intestine microbial metabolic activity.
33202270	7	1	theme	dietary	1123:1129	arg1	FVP					1097:1099	FVP	1097:1099	FVP	1097:1099	Our study suggested that FVP can be developed as a dietary supplement for intestine health promotion.
33202270	7	1	theme	dietary	1123:1129	arg1	supplement					1131:1140	a dietary supplement	1121:1140	a dietary supplement for intestine health promotion	1121:1171	Our study suggested that FVP can be developed as a dietary supplement for intestine health promotion.
33202270	5	2	theme	FVP	793:795	arg1	treatment					797:805	FVP treatment	793:805	FVP treatment in positive and negative ion mode	793:839	The metabolic profiling was detected by LC/MS and results showed 56 and 99 compounds were dramatically changed after FVP treatment in positive and negative ion mode, respectively.
33202270	4	3	theme	rRNA	515:518	arg1	sequencing					525:534	The 16 s rRNA gene sequencing	506:534	The 16 s rRNA gene sequencing	506:534	The 16 s rRNA gene sequencing revealed microbiota composition alteration caused by FVP, as the Firmicutes phylum increased while Bacteroidetes phylum slightly decreased.
33202270	2	4	used	used	293:296	arg2	experiment					278:287	the 28-days fed experiment	262:287	the 28-days fed experiment	262:287	In this study, the 28-days fed experiment was used to investigate gut microbime and metabolic profiling induced by FVP.
33202270	6	5	theme	metabolism	1007:1016	arg1	adjustment					940:949	the adjustment	936:949	the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways	936:1049	Annotation in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways displayed the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways after FVP treatment.
33202270	0	6	theme	metabolic	111:119	arg1	activity					121:128	intestine microbial metabolic activity	91:128	intestine microbial metabolic activity	91:128	Flammulina velutipes polysaccharide improves C57BL/6 mice gut health through regulation of intestine microbial metabolic activity.
33202270	4	7	theme	16 s	510:513	arg1	sequencing					525:534	The 16 s rRNA gene sequencing	506:534	The 16 s rRNA gene sequencing	506:534	The 16 s rRNA gene sequencing revealed microbiota composition alteration caused by FVP, as the Firmicutes phylum increased while Bacteroidetes phylum slightly decreased.
33202270	5	8	theme	metabolic	680:688	arg1	profiling					690:698	The metabolic profiling	676:698	The metabolic profiling	676:698	The metabolic profiling was detected by LC/MS and results showed 56 and 99 compounds were dramatically changed after FVP treatment in positive and negative ion mode, respectively.
33202270	6	9	theme	nucleotide	996:1005	arg1	metabolism					1007:1016	nucleotide metabolism	996:1016	nucleotide metabolism	996:1016	Annotation in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways displayed the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways after FVP treatment.
33202270	0	10	theme	microbial	101:109	arg1	activity					121:128	intestine microbial metabolic activity	91:128	intestine microbial metabolic activity	91:128	Flammulina velutipes polysaccharide improves C57BL/6 mice gut health through regulation of intestine microbial metabolic activity.
33202270	7	11	theme	health	1156:1161	arg1	promotion					1163:1171	intestine health promotion	1146:1171	intestine health promotion	1146:1171	Our study suggested that FVP can be developed as a dietary supplement for intestine health promotion.
33202270	3	12	theme	FVP-treated	487:497	arg1	group					499:503	FVP-treated group	487:503	FVP-treated group	487:503	After treatment, intestinal tissue section showed the higher villus height and villus height/crypt depth (V/C) value in FVP-treated group.
33202270	5	13	theme	negative	823:830	arg1	mode					836:839	positive and negative ion mode	810:839	positive and negative ion mode	810:839	The metabolic profiling was detected by LC/MS and results showed 56 and 99 compounds were dramatically changed after FVP treatment in positive and negative ion mode, respectively.
33202270	1	14	theme	gut	186:188	arg1	health					190:195	gut health	186:195	gut health	186:195	Flammulina velutipes polysaccharides (FVP) can improve gut health through gut microbiota and metabolism regulation.
33202270	0	15	theme	activity	121:128	arg1	regulation					77:86	regulation	77:86	regulation of intestine microbial metabolic activity	77:128	Flammulina velutipes polysaccharide improves C57BL/6 mice gut health through regulation of intestine microbial metabolic activity.
33202270	3	16	theme	higher	421:426	arg1	value					478:482	the higher villus height and villus height/crypt depth (V/C) value	417:482	the higher villus height and villus height/crypt depth (V/C) value in FVP-treated group	417:503	After treatment, intestinal tissue section showed the higher villus height and villus height/crypt depth (V/C) value in FVP-treated group.
33202270	3	17	from	value	478:482	arg1	group					499:503	FVP-treated group	487:503	FVP-treated group	487:503	After treatment, intestinal tissue section showed the higher villus height and villus height/crypt depth (V/C) value in FVP-treated group.
33202270	5	18	theme	ion	832:834	arg1	mode					836:839	positive and negative ion mode	810:839	positive and negative ion mode	810:839	The metabolic profiling was detected by LC/MS and results showed 56 and 99 compounds were dramatically changed after FVP treatment in positive and negative ion mode, respectively.
33202270	3	19	theme	height/crypt	453:464	arg1	depth					466:470	villus height/crypt depth	446:470	villus height/crypt depth	446:470	After treatment, intestinal tissue section showed the higher villus height and villus height/crypt depth (V/C) value in FVP-treated group.
33202270	0	20	theme	velutipes	11:19	arg1	polysaccharide					21:34	Flammulina velutipes polysaccharide	0:34	Flammulina velutipes polysaccharide	0:34	Flammulina velutipes polysaccharide improves C57BL/6 mice gut health through regulation of intestine microbial metabolic activity.
33202270	3	21	theme	height	435:440	arg1	value					478:482	the higher villus height and villus height/crypt depth (V/C) value	417:482	the higher villus height and villus height/crypt depth (V/C) value in FVP-treated group	417:503	After treatment, intestinal tissue section showed the higher villus height and villus height/crypt depth (V/C) value in FVP-treated group.
33202270	0	22	theme	Flammulina	0:9	arg1	velutipes					11:19	Flammulina velutipes	0:19	Flammulina velutipes polysaccharide	0:34	Flammulina velutipes polysaccharide improves C57BL/6 mice gut health through regulation of intestine microbial metabolic activity.
33202270	6	23	theme	metabolism	984:993	arg1	adjustment					940:949	the adjustment	936:949	the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways	936:1049	Annotation in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways displayed the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways after FVP treatment.
33202270	1	24	theme	gut	205:207	arg1	microbiota					209:218	gut microbiota	205:218	gut microbiota	205:218	Flammulina velutipes polysaccharides (FVP) can improve gut health through gut microbiota and metabolism regulation.
33202270	4	25	theme	composition	556:566	arg1	alteration					568:577	microbiota composition alteration	545:577	microbiota composition alteration caused by FVP	545:591	The 16 s rRNA gene sequencing revealed microbiota composition alteration caused by FVP, as the Firmicutes phylum increased while Bacteroidetes phylum slightly decreased.
33202270	2	26	theme	gut	313:315	arg1	microbime					317:325	gut microbime	313:325	gut microbime	313:325	In this study, the 28-days fed experiment was used to investigate gut microbime and metabolic profiling induced by FVP.
33202270	3	27	theme	intestinal	384:393	arg1	section					402:408	intestinal tissue section	384:408	intestinal tissue section	384:408	After treatment, intestinal tissue section showed the higher villus height and villus height/crypt depth (V/C) value in FVP-treated group.
33202270	6	28	theme	amino	973:977	arg1	metabolism					984:993	amino acid metabolism	973:993	amino acid metabolism	973:993	Annotation in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways displayed the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways after FVP treatment.
33202270	6	29	theme	Genes	892:896	arg1	KEGG					911:914	KEGG	911:914	KEGG	911:914	Annotation in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways displayed the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways after FVP treatment.
33202270	6	29	theme	Genes	892:896	arg1	Genomes					902:908	Genomes	902:908	Genomes (KEGG)	902:915	Annotation in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways displayed the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways after FVP treatment.
33202270	6	30	theme	acid	979:982	arg1	metabolism					984:993	amino acid metabolism	973:993	amino acid metabolism	973:993	Annotation in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways displayed the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways after FVP treatment.
33202270	0	31	theme	mice	53:56	arg1	health					62:67	C57BL/6 mice gut health	45:67	C57BL/6 mice gut health	45:67	Flammulina velutipes polysaccharide improves C57BL/6 mice gut health through regulation of intestine microbial metabolic activity.
33202270	6	32	dep	Encyclopedia	876:887	arg1	pathways					917:924	pathways	917:924	pathways	917:924	Annotation in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways displayed the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways after FVP treatment.
33202270	6	33	theme	FVP	1057:1059	arg1	treatment					1061:1069	FVP treatment	1057:1069	FVP treatment	1057:1069	Annotation in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways displayed the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways after FVP treatment.
33202270	3	34	theme	depth	466:470	arg1	value					478:482	the higher villus height and villus height/crypt depth (V/C) value	417:482	the higher villus height and villus height/crypt depth (V/C) value in FVP-treated group	417:503	After treatment, intestinal tissue section showed the higher villus height and villus height/crypt depth (V/C) value in FVP-treated group.
33202270	0	35	theme	C57BL/6	45:51	arg1	mice					53:56	C57BL/6 mice	45:56	C57BL/6 mice gut health	45:67	Flammulina velutipes polysaccharide improves C57BL/6 mice gut health through regulation of intestine microbial metabolic activity.
33202270	6	36	theme	metabolism	961:970	arg1	adjustment					940:949	the adjustment	936:949	the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways	936:1049	Annotation in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways displayed the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways after FVP treatment.
33202270	3	37	theme	villus	428:433	arg1	height					435:440	villus height	428:440	villus height	428:440	After treatment, intestinal tissue section showed the higher villus height and villus height/crypt depth (V/C) value in FVP-treated group.
33202270	3	37	theme	villus	428:433	arg1	V/C					473:475	V/C	473:475	V/C	473:475	After treatment, intestinal tissue section showed the higher villus height and villus height/crypt depth (V/C) value in FVP-treated group.
33202270	6	38	from	Annotation	856:865	arg1	Encyclopedia					876:887	Encyclopedia	876:887	Encyclopedia	876:887	Annotation in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways displayed the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways after FVP treatment.
33202270	6	38	from	Annotation	856:865	arg1	KEGG					911:914	KEGG	911:914	KEGG	911:914	Annotation in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways displayed the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways after FVP treatment.
33202270	6	38	from	Annotation	856:865	arg1	Genomes					902:908	Genomes	902:908	Genomes (KEGG)	902:915	Annotation in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways displayed the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways after FVP treatment.
33202270	5	39	theme	positive	810:817	arg1	mode					836:839	positive and negative ion mode	810:839	positive and negative ion mode	810:839	The metabolic profiling was detected by LC/MS and results showed 56 and 99 compounds were dramatically changed after FVP treatment in positive and negative ion mode, respectively.
33202270	4	40	theme	gene	520:523	arg1	sequencing					525:534	The 16 s rRNA gene sequencing	506:534	The 16 s rRNA gene sequencing	506:534	The 16 s rRNA gene sequencing revealed microbiota composition alteration caused by FVP, as the Firmicutes phylum increased while Bacteroidetes phylum slightly decreased.
33202270	3	41	theme	villus	446:451	arg1	depth					466:470	villus height/crypt depth	446:470	villus height/crypt depth	446:470	After treatment, intestinal tissue section showed the higher villus height and villus height/crypt depth (V/C) value in FVP-treated group.
33202270	1	42	theme	Flammulina	131:140	arg1	polysaccharides					152:166	Flammulina velutipes polysaccharides	131:166	Flammulina velutipes polysaccharides (FVP)	131:172	Flammulina velutipes polysaccharides (FVP) can improve gut health through gut microbiota and metabolism regulation.
33202270	1	42	theme	Flammulina	131:140	arg1	FVP					169:171	FVP	169:171	FVP	169:171	Flammulina velutipes polysaccharides (FVP) can improve gut health through gut microbiota and metabolism regulation.
33202270	0	43	theme	gut	58:60	arg1	health					62:67	C57BL/6 mice gut health	45:67	C57BL/6 mice gut health	45:67	Flammulina velutipes polysaccharide improves C57BL/6 mice gut health through regulation of intestine microbial metabolic activity.
33202270	4	44	theme	microbiota	545:554	arg1	composition					556:566	microbiota composition	545:566	microbiota composition alteration caused by FVP	545:591	The 16 s rRNA gene sequencing revealed microbiota composition alteration caused by FVP, as the Firmicutes phylum increased while Bacteroidetes phylum slightly decreased.
33202270	4	45	dep	Bacteroidetes	635:647	arg1	phylum					649:654	phylum	649:654	phylum	649:654	The 16 s rRNA gene sequencing revealed microbiota composition alteration caused by FVP, as the Firmicutes phylum increased while Bacteroidetes phylum slightly decreased.
33202270	1	46	theme	velutipes	142:150	arg1	polysaccharides					152:166	Flammulina velutipes polysaccharides	131:166	Flammulina velutipes polysaccharides (FVP)	131:172	Flammulina velutipes polysaccharides (FVP) can improve gut health through gut microbiota and metabolism regulation.
33202270	1	46	theme	velutipes	142:150	arg1	FVP					169:171	FVP	169:171	FVP	169:171	Flammulina velutipes polysaccharides (FVP) can improve gut health through gut microbiota and metabolism regulation.
33202270	7	47	theme	intestine	1146:1154	arg1	promotion					1163:1171	intestine health promotion	1146:1171	intestine health promotion	1146:1171	Our study suggested that FVP can be developed as a dietary supplement for intestine health promotion.
33202270	2	48	theme	fed	274:276	arg1	experiment					278:287	the 28-days fed experiment	262:287	the 28-days fed experiment	262:287	In this study, the 28-days fed experiment was used to investigate gut microbime and metabolic profiling induced by FVP.
33202270	6	49	theme	pathways	1042:1049	arg1	adjustment					940:949	the adjustment	936:949	the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways	936:1049	Annotation in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways displayed the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways after FVP treatment.
33202270	2	50	theme	28-days	266:272	arg1	experiment					278:287	the 28-days fed experiment	262:287	the 28-days fed experiment	262:287	In this study, the 28-days fed experiment was used to investigate gut microbime and metabolic profiling induced by FVP.
33202270	2	51	theme	metabolic	331:339	arg1	profiling					341:349	metabolic profiling	331:349	metabolic profiling	331:349	In this study, the 28-days fed experiment was used to investigate gut microbime and metabolic profiling induced by FVP.
33202270	6	52	theme	basic	1036:1040	arg1	pathways					1042:1049	other related basic pathways	1022:1049	other related basic pathways	1022:1049	Annotation in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways displayed the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways after FVP treatment.
33202270	5	53	dep	showed	734:739	arg1	changed					779:785	changed	779:785	changed	779:785	The metabolic profiling was detected by LC/MS and results showed 56 and 99 compounds were dramatically changed after FVP treatment in positive and negative ion mode, respectively.
33202270	1	54	dep	microbiota	209:218	arg1	regulation					235:244	regulation	235:244	regulation	235:244	Flammulina velutipes polysaccharides (FVP) can improve gut health through gut microbiota and metabolism regulation.
33202270	6	55	theme	related	1028:1034	arg1	pathways					1042:1049	other related basic pathways	1022:1049	other related basic pathways	1022:1049	Annotation in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways displayed the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways after FVP treatment.
33202270	5	56	from	treatment	797:805	arg1	mode					836:839	positive and negative ion mode	810:839	positive and negative ion mode	810:839	The metabolic profiling was detected by LC/MS and results showed 56 and 99 compounds were dramatically changed after FVP treatment in positive and negative ion mode, respectively.
33202270	6	57	theme	other	1022:1026	arg1	pathways					1042:1049	other related basic pathways	1022:1049	other related basic pathways	1022:1049	Annotation in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways displayed the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways after FVP treatment.
33202270	3	58	theme	tissue	395:400	arg1	section					402:408	intestinal tissue section	384:408	intestinal tissue section	384:408	After treatment, intestinal tissue section showed the higher villus height and villus height/crypt depth (V/C) value in FVP-treated group.
33202270	6	59	theme	energy	954:959	arg1	metabolism					961:970	energy metabolism	954:970	energy metabolism	954:970	Annotation in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways displayed the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways after FVP treatment.
34990944	6	0	theme	excessive	903:911	arg1	composites					913:922	excessive composites	903:922	excessive composites	903:922	Fluorescence microscopy and scanning electron microscopy (SEM) results confirmed that the most of excessive β-CD was adsorbed on the surface of emulsion droplets as crystals, formed thick protective shell in β-CD emulsions, while the most of excessive composites were distributed in the aqueous phase forming a stable network structure in CEO/β-CD emulsions.
34990944	7	1	theme	droplet	1121:1127	arg1	size					1129:1132	droplet size	1121:1132	droplet size	1121:1132	It caused these two emulsions had different rheological properties, and different changing trends in droplet size.
34990944	1	2	theme	CEO	166:168	arg1	composite					193:201	a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite	134:201	a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite with a high embedding rate (91.74 ± 0.82%)	134:244	Here, a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite with a high embedding rate (91.74 ± 0.82%) was prepared.
34990944	4	3	theme	CEO/β-CD	497:504	arg1	emulsions					506:514	The CEO/β-CD emulsions	493:514	The CEO/β-CD emulsions	493:514	The CEO/β-CD emulsions had better storage stability.
34990944	6	4	theme	protective	849:858	arg1	shell					860:864	thick protective shell	843:864	thick protective shell in β-CD emulsions	843:882	Fluorescence microscopy and scanning electron microscopy (SEM) results confirmed that the most of excessive β-CD was adsorbed on the surface of emulsion droplets as crystals, formed thick protective shell in β-CD emulsions, while the most of excessive composites were distributed in the aqueous phase forming a stable network structure in CEO/β-CD emulsions.
34990944	5	5	dep	confirmed	566:574	arg1	had					630:632	had	630:632	had shear thinning phenomenon	630:658	Rheological results confirmed the emulsions were all gel-like elastic emulsions and had shear thinning phenomenon.
34990944	5	5	dep	confirmed	566:574	arg1	emulsions					616:624	all gel-like elastic emulsions	595:624	all gel-like elastic emulsions	595:624	Rheological results confirmed the emulsions were all gel-like elastic emulsions and had shear thinning phenomenon.
34990944	7	6	dep	caused	1023:1028	arg1	had					1050:1052	had	1050:1052	caused these two emulsions had different rheological properties, and different changing trends in droplet size	1023:1132	It caused these two emulsions had different rheological properties, and different changing trends in droplet size.
34990944	5	7	theme	gel-like	599:606	arg1	emulsions					616:624	all gel-like elastic emulsions	595:624	all gel-like elastic emulsions	595:624	Rheological results confirmed the emulsions were all gel-like elastic emulsions and had shear thinning phenomenon.
34990944	6	8	theme	network	979:985	arg1	structure					987:995	a stable network structure	970:995	a stable network structure	970:995	Fluorescence microscopy and scanning electron microscopy (SEM) results confirmed that the most of excessive β-CD was adsorbed on the surface of emulsion droplets as crystals, formed thick protective shell in β-CD emulsions, while the most of excessive composites were distributed in the aqueous phase forming a stable network structure in CEO/β-CD emulsions.
34990944	0	9	theme	comparison	110:119	arg1	study					121:125	A comparison study	108:125	A comparison study	108:125	Pickering emulsions stabilized by β-cyclodextrin and cinnamaldehyde essential oil/β-cyclodextrin composite: A comparison study.
34990944	5	10	theme	elastic	608:614	arg1	emulsions					616:624	all gel-like elastic emulsions	595:624	all gel-like elastic emulsions	595:624	Rheological results confirmed the emulsions were all gel-like elastic emulsions and had shear thinning phenomenon.
34990944	1	11	theme	essential	151:159	arg1	composite					193:201	a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite	134:201	a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite with a high embedding rate (91.74 ± 0.82%)	134:244	Here, a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite with a high embedding rate (91.74 ± 0.82%) was prepared.
34990944	7	12	theme	different	1092:1100	arg1	trends					1111:1116	different changing trends	1092:1116	different changing trends in droplet size	1092:1132	It caused these two emulsions had different rheological properties, and different changing trends in droplet size.
34990944	0	13	dep	study	121:125	arg1	emulsions					10:18	Pickering emulsions	0:18	Pickering emulsions stabilized by β-cyclodextrin and cinnamaldehyde essential oil/β-cyclodextrin composite	0:105	Pickering emulsions stabilized by β-cyclodextrin and cinnamaldehyde essential oil/β-cyclodextrin composite: A comparison study.
34990944	1	14	theme	/β-Cyclodextrin	170:184	arg1	composite					193:201	a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite	134:201	a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite with a high embedding rate (91.74 ± 0.82%)	134:244	Here, a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite with a high embedding rate (91.74 ± 0.82%) was prepared.
34990944	6	15	theme	stable	972:977	arg1	structure					987:995	a stable network structure	970:995	a stable network structure	970:995	Fluorescence microscopy and scanning electron microscopy (SEM) results confirmed that the most of excessive β-CD was adsorbed on the surface of emulsion droplets as crystals, formed thick protective shell in β-CD emulsions, while the most of excessive composites were distributed in the aqueous phase forming a stable network structure in CEO/β-CD emulsions.
34990944	7	16	theme	changing	1102:1109	arg1	trends					1111:1116	different changing trends	1092:1116	different changing trends in droplet size	1092:1132	It caused these two emulsions had different rheological properties, and different changing trends in droplet size.
34990944	4	17	contain	had	516:518	arg1	emulsions					506:514	The CEO/β-CD emulsions	493:514	The CEO/β-CD emulsions	493:514	The CEO/β-CD emulsions had better storage stability.
34990944	4	17	contain	had	516:518	arg2	stability					535:543	better storage stability	520:543	better storage stability	520:543	The CEO/β-CD emulsions had better storage stability.
34990944	7	18	from	properties	1076:1085	arg1	size					1129:1132	droplet size	1121:1132	droplet size	1121:1132	It caused these two emulsions had different rheological properties, and different changing trends in droplet size.
34990944	6	19	from	shell	860:864	arg1	emulsions					874:882	β-CD emulsions	869:882	β-CD emulsions	869:882	Fluorescence microscopy and scanning electron microscopy (SEM) results confirmed that the most of excessive β-CD was adsorbed on the surface of emulsion droplets as crystals, formed thick protective shell in β-CD emulsions, while the most of excessive composites were distributed in the aqueous phase forming a stable network structure in CEO/β-CD emulsions.
34990944	5	20	theme	shear	634:638	arg1	phenomenon					649:658	shear thinning phenomenon	634:658	shear thinning phenomenon	634:658	Rheological results confirmed the emulsions were all gel-like elastic emulsions and had shear thinning phenomenon.
34990944	1	21	theme	β-CD	187:190	arg1	composite					193:201	a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite	134:201	a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite with a high embedding rate (91.74 ± 0.82%)	134:244	Here, a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite with a high embedding rate (91.74 ± 0.82%) was prepared.
34990944	0	22	theme	Pickering	0:8	arg1	emulsions					10:18	Pickering emulsions	0:18	Pickering emulsions stabilized by β-cyclodextrin and cinnamaldehyde essential oil/β-cyclodextrin composite	0:105	Pickering emulsions stabilized by β-cyclodextrin and cinnamaldehyde essential oil/β-cyclodextrin composite: A comparison study.
34990944	5	23	theme	thinning	640:647	arg1	phenomenon					649:658	shear thinning phenomenon	634:658	shear thinning phenomenon	634:658	Rheological results confirmed the emulsions were all gel-like elastic emulsions and had shear thinning phenomenon.
34990944	7	24	theme	rheological	1064:1074	arg1	properties					1076:1085	different rheological properties	1054:1085	different rheological properties	1054:1085	It caused these two emulsions had different rheological properties, and different changing trends in droplet size.
34990944	6	25	theme	electron	698:705	arg1	SEM					719:721	SEM	719:721	SEM	719:721	Fluorescence microscopy and scanning electron microscopy (SEM) results confirmed that the most of excessive β-CD was adsorbed on the surface of emulsion droplets as crystals, formed thick protective shell in β-CD emulsions, while the most of excessive composites were distributed in the aqueous phase forming a stable network structure in CEO/β-CD emulsions.
34990944	6	25	theme	electron	698:705	arg1	microscopy					707:716	scanning electron microscopy	689:716	scanning electron microscopy (SEM)	689:722	Fluorescence microscopy and scanning electron microscopy (SEM) results confirmed that the most of excessive β-CD was adsorbed on the surface of emulsion droplets as crystals, formed thick protective shell in β-CD emulsions, while the most of excessive composites were distributed in the aqueous phase forming a stable network structure in CEO/β-CD emulsions.
34990944	6	26	theme	excessive	759:767	arg1	β-CD					769:772	excessive β-CD	759:772	excessive β-CD	759:772	Fluorescence microscopy and scanning electron microscopy (SEM) results confirmed that the most of excessive β-CD was adsorbed on the surface of emulsion droplets as crystals, formed thick protective shell in β-CD emulsions, while the most of excessive composites were distributed in the aqueous phase forming a stable network structure in CEO/β-CD emulsions.
34990944	6	27	theme	β-CD	869:872	arg1	emulsions					874:882	β-CD emulsions	869:882	β-CD emulsions	869:882	Fluorescence microscopy and scanning electron microscopy (SEM) results confirmed that the most of excessive β-CD was adsorbed on the surface of emulsion droplets as crystals, formed thick protective shell in β-CD emulsions, while the most of excessive composites were distributed in the aqueous phase forming a stable network structure in CEO/β-CD emulsions.
34990944	6	28	theme	scanning	689:696	arg1	SEM					719:721	SEM	719:721	SEM	719:721	Fluorescence microscopy and scanning electron microscopy (SEM) results confirmed that the most of excessive β-CD was adsorbed on the surface of emulsion droplets as crystals, formed thick protective shell in β-CD emulsions, while the most of excessive composites were distributed in the aqueous phase forming a stable network structure in CEO/β-CD emulsions.
34990944	6	28	theme	scanning	689:696	arg1	microscopy					707:716	scanning electron microscopy	689:716	scanning electron microscopy (SEM)	689:722	Fluorescence microscopy and scanning electron microscopy (SEM) results confirmed that the most of excessive β-CD was adsorbed on the surface of emulsion droplets as crystals, formed thick protective shell in β-CD emulsions, while the most of excessive composites were distributed in the aqueous phase forming a stable network structure in CEO/β-CD emulsions.
34990944	6	29	theme	aqueous	948:954	arg1	phase					956:960	the aqueous phase	944:960	the aqueous phase forming a stable network structure in CEO/β-CD emulsions	944:1017	Fluorescence microscopy and scanning electron microscopy (SEM) results confirmed that the most of excessive β-CD was adsorbed on the surface of emulsion droplets as crystals, formed thick protective shell in β-CD emulsions, while the most of excessive composites were distributed in the aqueous phase forming a stable network structure in CEO/β-CD emulsions.
34990944	1	30	with	composite	193:201	arg1	rate					225:228	a high embedding rate	208:228	a high embedding rate (91.74 ± 0.82%)	208:244	Here, a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite with a high embedding rate (91.74 ± 0.82%) was prepared.
34990944	1	30	with	composite	193:201	arg1	%					243:243	91.74 ± 0.82%	231:243	91.74 ± 0.82%	231:243	Here, a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite with a high embedding rate (91.74 ± 0.82%) was prepared.
34990944	3	31	theme	different	428:436	arg1	concentrations					438:451	different concentrations	428:451	different concentrations (1-5%)	428:458	Pickering emulsions prepared by β-CD and CEO/β-CD at different concentrations (1-5%) were comparatively investigated.
34990944	3	31	theme	different	428:436	arg1	%					457:457	1-5%	454:457	1-5%	454:457	Pickering emulsions prepared by β-CD and CEO/β-CD at different concentrations (1-5%) were comparatively investigated.
34990944	7	32	from	trends	1111:1116	arg1	size					1129:1132	droplet size	1121:1132	droplet size	1121:1132	It caused these two emulsions had different rheological properties, and different changing trends in droplet size.
34990944	7	33	theme	different	1054:1062	arg1	properties					1076:1085	different rheological properties	1054:1085	different rheological properties	1054:1085	It caused these two emulsions had different rheological properties, and different changing trends in droplet size.
34990944	1	34	theme	high	210:213	arg1	rate					225:228	a high embedding rate	208:228	a high embedding rate (91.74 ± 0.82%)	208:244	Here, a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite with a high embedding rate (91.74 ± 0.82%) was prepared.
34990944	1	34	theme	high	210:213	arg1	%					243:243	91.74 ± 0.82%	231:243	91.74 ± 0.82%	231:243	Here, a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite with a high embedding rate (91.74 ± 0.82%) was prepared.
34990944	3	35	theme	Pickering	375:383	arg1	emulsions					385:393	Pickering emulsions	375:393	Pickering emulsions prepared by β-CD and CEO/β-CD at different concentrations (1-5%)	375:458	Pickering emulsions prepared by β-CD and CEO/β-CD at different concentrations (1-5%) were comparatively investigated.
34990944	6	36	theme	emulsion	805:812	arg1	droplets					814:821	emulsion droplets	805:821	emulsion droplets as crystals, formed thick protective shell in β-CD emulsions	805:882	Fluorescence microscopy and scanning electron microscopy (SEM) results confirmed that the most of excessive β-CD was adsorbed on the surface of emulsion droplets as crystals, formed thick protective shell in β-CD emulsions, while the most of excessive composites were distributed in the aqueous phase forming a stable network structure in CEO/β-CD emulsions.
34990944	0	37	theme	oil/β-cyclodextrin	78:95	arg1	composite					97:105	cinnamaldehyde essential oil/β-cyclodextrin composite	53:105	cinnamaldehyde essential oil/β-cyclodextrin composite	53:105	Pickering emulsions stabilized by β-cyclodextrin and cinnamaldehyde essential oil/β-cyclodextrin composite: A comparison study.
34990944	0	38	theme	cinnamaldehyde	53:66	arg1	composite					97:105	cinnamaldehyde essential oil/β-cyclodextrin composite	53:105	cinnamaldehyde essential oil/β-cyclodextrin composite	53:105	Pickering emulsions stabilized by β-cyclodextrin and cinnamaldehyde essential oil/β-cyclodextrin composite: A comparison study.
34990944	4	39	theme	storage	527:533	arg1	stability					535:543	better storage stability	520:543	better storage stability	520:543	The CEO/β-CD emulsions had better storage stability.
34990944	6	40	theme	thick	843:847	arg1	shell					860:864	thick protective shell	843:864	thick protective shell in β-CD emulsions	843:882	Fluorescence microscopy and scanning electron microscopy (SEM) results confirmed that the most of excessive β-CD was adsorbed on the surface of emulsion droplets as crystals, formed thick protective shell in β-CD emulsions, while the most of excessive composites were distributed in the aqueous phase forming a stable network structure in CEO/β-CD emulsions.
34990944	5	41	theme	Rheological	546:556	arg1	results					558:564	Rheological results	546:564	Rheological results	546:564	Rheological results confirmed the emulsions were all gel-like elastic emulsions and had shear thinning phenomenon.
34990944	4	42	theme	better	520:525	arg1	stability					535:543	better storage stability	520:543	better storage stability	520:543	The CEO/β-CD emulsions had better storage stability.
34990944	6	43	theme	droplets	814:821	arg1	surface					794:800	the surface	790:800	the surface of emulsion droplets as crystals, formed thick protective shell in β-CD emulsions	790:882	Fluorescence microscopy and scanning electron microscopy (SEM) results confirmed that the most of excessive β-CD was adsorbed on the surface of emulsion droplets as crystals, formed thick protective shell in β-CD emulsions, while the most of excessive composites were distributed in the aqueous phase forming a stable network structure in CEO/β-CD emulsions.
34990944	1	44	theme	cinnamaldehyde	136:149	arg1	composite					193:201	a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite	134:201	a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite with a high embedding rate (91.74 ± 0.82%)	134:244	Here, a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite with a high embedding rate (91.74 ± 0.82%) was prepared.
34990944	6	45	theme	Fluorescence	661:672	arg1	microscopy					674:683	Fluorescence microscopy	661:683	Fluorescence microscopy	661:683	Fluorescence microscopy and scanning electron microscopy (SEM) results confirmed that the most of excessive β-CD was adsorbed on the surface of emulsion droplets as crystals, formed thick protective shell in β-CD emulsions, while the most of excessive composites were distributed in the aqueous phase forming a stable network structure in CEO/β-CD emulsions.
34990944	1	46	theme	embedding	215:223	arg1	rate					225:228	a high embedding rate	208:228	a high embedding rate (91.74 ± 0.82%)	208:244	Here, a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite with a high embedding rate (91.74 ± 0.82%) was prepared.
34990944	1	46	theme	embedding	215:223	arg1	%					243:243	91.74 ± 0.82%	231:243	91.74 ± 0.82%	231:243	Here, a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite with a high embedding rate (91.74 ± 0.82%) was prepared.
34990944	2	47	dep	Fourier	295:301	arg1	transform					303:311	transform	303:311	transform infrared spectrometer (FT-IR) and X-ray diffractometer (XRD)	303:372	Its structure was characterized by Fourier transform infrared spectrometer (FT-IR) and X-ray diffractometer (XRD).
34990944	2	48	dep	transform	303:311	arg1	infrared					313:320	infrared	313:320	transform infrared spectrometer (FT-IR) and X-ray diffractometer (XRD)	303:372	Its structure was characterized by Fourier transform infrared spectrometer (FT-IR) and X-ray diffractometer (XRD).
34990944	1	49	theme	oil	161:163	arg1	composite					193:201	a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite	134:201	a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite with a high embedding rate (91.74 ± 0.82%)	134:244	Here, a cinnamaldehyde essential oil (CEO)/β-Cyclodextrin (β-CD) composite with a high embedding rate (91.74 ± 0.82%) was prepared.
34990944	0	50	theme	essential	68:76	arg1	composite					97:105	cinnamaldehyde essential oil/β-cyclodextrin composite	53:105	cinnamaldehyde essential oil/β-cyclodextrin composite	53:105	Pickering emulsions stabilized by β-cyclodextrin and cinnamaldehyde essential oil/β-cyclodextrin composite: A comparison study.
34990944	6	51	dep	microscopy	674:683	arg1	results					724:730	results	724:730	results	724:730	Fluorescence microscopy and scanning electron microscopy (SEM) results confirmed that the most of excessive β-CD was adsorbed on the surface of emulsion droplets as crystals, formed thick protective shell in β-CD emulsions, while the most of excessive composites were distributed in the aqueous phase forming a stable network structure in CEO/β-CD emulsions.
34990944	2	52	theme	X-ray	347:351	arg1	XRD					369:371	XRD	369:371	XRD	369:371	Its structure was characterized by Fourier transform infrared spectrometer (FT-IR) and X-ray diffractometer (XRD).
34990944	2	52	theme	X-ray	347:351	arg1	diffractometer					353:366	X-ray diffractometer	347:366	X-ray diffractometer (XRD)	347:372	Its structure was characterized by Fourier transform infrared spectrometer (FT-IR) and X-ray diffractometer (XRD).
34990944	6	53	theme	CEO/β-CD	1000:1007	arg1	emulsions					1009:1017	CEO/β-CD emulsions	1000:1017	CEO/β-CD emulsions	1000:1017	Fluorescence microscopy and scanning electron microscopy (SEM) results confirmed that the most of excessive β-CD was adsorbed on the surface of emulsion droplets as crystals, formed thick protective shell in β-CD emulsions, while the most of excessive composites were distributed in the aqueous phase forming a stable network structure in CEO/β-CD emulsions.
32607666	10	0	theme	immunoelectrophoresis	1529:1549	arg1	analyses					1551:1558	The SDS-PAGE, immunodiffusion, and linear immunoelectrophoresis analyses	1487:1558	The SDS-PAGE, immunodiffusion, and linear immunoelectrophoresis analyses	1487:1558	The SDS-PAGE, immunodiffusion, and linear immunoelectrophoresis analyses showed that in strain KM018 (pRK415-p60025), the wild-type LpsI+ LpsII+ profile was restored.
32607666	0	1	from	formation	60:68	arg1	Sp245					132:136	Azospirillum brasilense Sp245	108:136	Azospirillum brasilense Sp245	108:136	Plasmid gene for putative integral membrane protein affects formation of lipopolysaccharide and motility in Azospirillum brasilense Sp245.
32607666	6	2	theme	Calcofluor-binding	838:855	arg1	polysaccharides					857:871	Calcofluor-binding polysaccharides	838:871	Calcofluor-binding polysaccharides	838:871	The mutant was no longer able to produce Calcofluor-binding polysaccharides and LpsII.
32607666	7	3	theme	experimental	907:918	arg1	data					920:923	the limited experimental data	895:923	the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla	895:1019	Because of the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla, the aim of this study was to identify and examine in more detail the coding sequence of strain Sp245, inactivated in the mutant.
32607666	8	4	theme	coding	1195:1200	arg1	sequence					1202:1209	a coding sequence	1193:1209	a coding sequence for a putative integral membrane protein of unknown function (AZOBR_p60025) located in the sixth plasmid of Sp245	1193:1323	We found that pJFF350 was integrated into a coding sequence for a putative integral membrane protein of unknown function (AZOBR_p60025) located in the sixth plasmid of Sp245.
32607666	8	4	theme	coding	1195:1200	arg1	AZOBR_p60025					1273:1284	AZOBR_p60025	1273:1284	AZOBR_p60025	1273:1284	We found that pJFF350 was integrated into a coding sequence for a putative integral membrane protein of unknown function (AZOBR_p60025) located in the sixth plasmid of Sp245.
32607666	8	5	theme	function	1263:1270	arg1	protein					1244:1250	a putative integral membrane protein	1215:1250	a putative integral membrane protein of unknown function	1215:1270	We found that pJFF350 was integrated into a coding sequence for a putative integral membrane protein of unknown function (AZOBR_p60025) located in the sixth plasmid of Sp245.
32607666	12	6	dep	single-cell	1878:1888	arg1	the					1874:1876	the	1874:1876	the	1874:1876	Thus, the AZOBR_p60025-encoded protein significantly affects the composition of the major cell-surface glycopolymers and the single-cell and social motility of azospirilla.
32607666	11	7	theme	complemented	1658:1669	arg1	mutant					1671:1676	The complemented mutant	1654:1676	The complemented mutant	1654:1676	The complemented mutant had a Cal+ phenotype and it was capable of swimming and swarming motility.
32607666	5	8	theme	random	671:676	arg1	mutagenesis					688:698	random Omegon-Km mutagenesis	671:698	random Omegon-Km mutagenesis	671:698	Previously, using vector pJFF350 for random Omegon-Km mutagenesis, we constructed a mutant of Sp245 named KM018 that still possessed flagella, although paralyzed.
32607666	4	9	contain	containing	594:603	arg1	LpsI					577:580	LpsI	577:580	LpsI	577:580	The facultative plant endophyte A. brasilense Sp245 produces two antigenically different LPS, LpsI, and LpsII, containing identical O-polysaccharides.
32607666	4	9	contain	containing	594:603	arg1	LpsII					587:591	LpsII	587:591	LpsII	587:591	The facultative plant endophyte A. brasilense Sp245 produces two antigenically different LPS, LpsI, and LpsII, containing identical O-polysaccharides.
32607666	4	9	contain	containing	594:603	arg1	LPS					572:574	two antigenically different LPS	544:574	two antigenically different LPS	544:574	The facultative plant endophyte A. brasilense Sp245 produces two antigenically different LPS, LpsI, and LpsII, containing identical O-polysaccharides.
32607666	4	9	contain	containing	594:603	arg2	O-polysaccharides					615:631	identical O-polysaccharides	605:631	identical O-polysaccharides	605:631	The facultative plant endophyte A. brasilense Sp245 produces two antigenically different LPS, LpsI, and LpsII, containing identical O-polysaccharides.
32607666	9	10	theme	genetic	1446:1452	arg1	complementation					1454:1468	the genetic complementation	1442:1468	the genetic complementation of mutant KM018	1442:1484	To clarify the role of the putative protein, we cloned AZOBR_p60025 in the expression vector pRK415 and used it for the genetic complementation of mutant KM018.
32607666	8	11	theme	putative	1217:1224	arg1	protein					1244:1250	a putative integral membrane protein	1215:1250	a putative integral membrane protein of unknown function	1215:1270	We found that pJFF350 was integrated into a coding sequence for a putative integral membrane protein of unknown function (AZOBR_p60025) located in the sixth plasmid of Sp245.
32607666	8	12	theme	located	1287:1293	arg1	sequence					1202:1209	a coding sequence	1193:1209	a coding sequence for a putative integral membrane protein of unknown function (AZOBR_p60025) located in the sixth plasmid of Sp245	1193:1323	We found that pJFF350 was integrated into a coding sequence for a putative integral membrane protein of unknown function (AZOBR_p60025) located in the sixth plasmid of Sp245.
32607666	8	12	theme	located	1287:1293	arg1	AZOBR_p60025					1273:1284	AZOBR_p60025	1273:1284	AZOBR_p60025	1273:1284	We found that pJFF350 was integrated into a coding sequence for a putative integral membrane protein of unknown function (AZOBR_p60025) located in the sixth plasmid of Sp245.
32607666	7	13	theme	strain	1110:1115	arg1	Sp245					1117:1121	strain Sp245	1110:1121	strain Sp245	1110:1121	Because of the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla, the aim of this study was to identify and examine in more detail the coding sequence of strain Sp245, inactivated in the mutant.
32607666	8	14	theme	membrane	1235:1242	arg1	protein					1244:1250	a putative integral membrane protein	1215:1250	a putative integral membrane protein of unknown function	1215:1270	We found that pJFF350 was integrated into a coding sequence for a putative integral membrane protein of unknown function (AZOBR_p60025) located in the sixth plasmid of Sp245.
32607666	10	15	theme	LpsII+	1625:1630	arg1	profile					1632:1638	the wild-type LpsI+ LpsII+ profile	1605:1638	the wild-type LpsI+ LpsII+ profile	1605:1638	The SDS-PAGE, immunodiffusion, and linear immunoelectrophoresis analyses showed that in strain KM018 (pRK415-p60025), the wild-type LpsI+ LpsII+ profile was restored.
32607666	1	16	theme	polar	217:221	arg1	flagella					235:242	polar and lateral flagella	217:242	polar and lateral flagella	217:242	The bacterium Azospirillum brasilense can swim and swarm owing to the work of polar and lateral flagella.
32607666	4	17	theme	facultative	487:497	arg1	endophyte					505:513	The facultative plant endophyte A. brasilense Sp245	483:533	The facultative plant endophyte A. brasilense Sp245	483:533	The facultative plant endophyte A. brasilense Sp245 produces two antigenically different LPS, LpsI, and LpsII, containing identical O-polysaccharides.
32607666	10	18	theme	wild-type	1609:1617	arg1	profile					1632:1638	the wild-type LpsI+ LpsII+ profile	1605:1638	the wild-type LpsI+ LpsII+ profile	1605:1638	The SDS-PAGE, immunodiffusion, and linear immunoelectrophoresis analyses showed that in strain KM018 (pRK415-p60025), the wild-type LpsI+ LpsII+ profile was restored.
32607666	3	19	with	bacteria	461:468	arg1	plants					475:480	plants	475:480	plants	475:480	Motility and surface glycopolymers are important for the interactions of plant-associated bacteria with plants.
32607666	8	20	theme	Sp245	1319:1323	arg1	plasmid					1308:1314	the sixth plasmid	1298:1314	the sixth plasmid of Sp245	1298:1323	We found that pJFF350 was integrated into a coding sequence for a putative integral membrane protein of unknown function (AZOBR_p60025) located in the sixth plasmid of Sp245.
32607666	7	21	theme	more	1075:1078	arg1	detail					1080:1085	more detail	1075:1085	more detail	1075:1085	Because of the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla, the aim of this study was to identify and examine in more detail the coding sequence of strain Sp245, inactivated in the mutant.
32607666	2	22	theme	surface	255:261	arg1	glycopolymers					263:275	Its major surface glycopolymers	245:275	Its major surface glycopolymers	245:275	Its major surface glycopolymers consist of lipopolysaccharides (LPS) and Calcofluor-binding polysaccharides (Cal+ phenotype).
32607666	7	23	theme	study	1038:1042	arg1	aim					1026:1028	the aim	1022:1028	the aim of this study	1022:1042	Because of the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla, the aim of this study was to identify and examine in more detail the coding sequence of strain Sp245, inactivated in the mutant.
32607666	1	24	theme	bacterium	143:151	arg1	brasilense					166:175	The bacterium Azospirillum brasilense	139:175	The bacterium Azospirillum brasilense	139:175	The bacterium Azospirillum brasilense can swim and swarm owing to the work of polar and lateral flagella.
32607666	7	25	from	motility	997:1004	arg1	azospirilla					1009:1019	azospirilla	1009:1019	azospirilla	1009:1019	Because of the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla, the aim of this study was to identify and examine in more detail the coding sequence of strain Sp245, inactivated in the mutant.
32607666	7	26	from	azospirilla	1009:1019	arg1	aspects					940:946	the genetic aspects	928:946	the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla	928:1019	Because of the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla, the aim of this study was to identify and examine in more detail the coding sequence of strain Sp245, inactivated in the mutant.
32607666	8	27	from	located	1287:1293	arg1	plasmid					1308:1314	the sixth plasmid	1298:1314	the sixth plasmid of Sp245	1298:1323	We found that pJFF350 was integrated into a coding sequence for a putative integral membrane protein of unknown function (AZOBR_p60025) located in the sixth plasmid of Sp245.
32607666	0	28	theme	lipopolysaccharide	73:90	arg1	formation					60:68	formation	60:68	formation of lipopolysaccharide and motility in Azospirillum brasilense Sp245	60:136	Plasmid gene for putative integral membrane protein affects formation of lipopolysaccharide and motility in Azospirillum brasilense Sp245.
32607666	0	29	theme	motility	96:103	arg1	formation					60:68	formation	60:68	formation of lipopolysaccharide and motility in Azospirillum brasilense Sp245	60:136	Plasmid gene for putative integral membrane protein affects formation of lipopolysaccharide and motility in Azospirillum brasilense Sp245.
32607666	8	30	from	plasmid	1308:1314	arg1	located					1287:1293	located	1287:1293	located	1287:1293	We found that pJFF350 was integrated into a coding sequence for a putative integral membrane protein of unknown function (AZOBR_p60025) located in the sixth plasmid of Sp245.
32607666	0	31	theme	Azospirillum	108:119	arg1	Sp245					132:136	Azospirillum brasilense Sp245	108:136	Azospirillum brasilense Sp245	108:136	Plasmid gene for putative integral membrane protein affects formation of lipopolysaccharide and motility in Azospirillum brasilense Sp245.
32607666	7	32	theme	motility	997:1004	arg1	aspects					940:946	the genetic aspects	928:946	the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla	928:1019	Because of the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla, the aim of this study was to identify and examine in more detail the coding sequence of strain Sp245, inactivated in the mutant.
32607666	4	33	dep	endophyte	505:513	arg1	Sp245					529:533	Sp245	529:533	Sp245	529:533	The facultative plant endophyte A. brasilense Sp245 produces two antigenically different LPS, LpsI, and LpsII, containing identical O-polysaccharides.
32607666	3	34	theme	plant-associated	444:459	arg1	bacteria					461:468	plant-associated bacteria	444:468	plant-associated bacteria with plants	444:480	Motility and surface glycopolymers are important for the interactions of plant-associated bacteria with plants.
32607666	0	35	theme	Plasmid	0:6	arg1	gene					8:11	Plasmid gene	0:11	Plasmid gene for putative integral membrane protein	0:50	Plasmid gene for putative integral membrane protein affects formation of lipopolysaccharide and motility in Azospirillum brasilense Sp245.
32607666	3	36	theme	surface	384:390	arg1	glycopolymers					392:404	surface glycopolymers	384:404	surface glycopolymers	384:404	Motility and surface glycopolymers are important for the interactions of plant-associated bacteria with plants.
32607666	12	37	theme	single-cell	1878:1888	arg1	composition					1818:1828	the composition	1814:1828	the composition of the major cell-surface glycopolymers and the single-cell and social motility of azospirilla	1814:1923	Thus, the AZOBR_p60025-encoded protein significantly affects the composition of the major cell-surface glycopolymers and the single-cell and social motility of azospirilla.
32607666	7	38	dep	was	1044:1046	arg1	inactivated					1124:1134	inactivated	1124:1134	inactivated in the mutant	1124:1148	Because of the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla, the aim of this study was to identify and examine in more detail the coding sequence of strain Sp245, inactivated in the mutant.
32607666	0	39	theme	integral	26:33	arg1	protein					44:50	putative integral membrane protein	17:50	putative integral membrane protein	17:50	Plasmid gene for putative integral membrane protein affects formation of lipopolysaccharide and motility in Azospirillum brasilense Sp245.
32607666	9	40	theme	protein	1362:1368	arg1	role					1341:1344	the role	1337:1344	the role of the putative protein	1337:1368	To clarify the role of the putative protein, we cloned AZOBR_p60025 in the expression vector pRK415 and used it for the genetic complementation of mutant KM018.
32607666	12	41	theme	social	1894:1899	arg1	motility					1901:1908	social motility	1894:1908	social motility	1894:1908	Thus, the AZOBR_p60025-encoded protein significantly affects the composition of the major cell-surface glycopolymers and the single-cell and social motility of azospirilla.
32607666	10	42	theme	linear	1522:1527	arg1	immunoelectrophoresis					1529:1549	linear immunoelectrophoresis	1522:1549	linear immunoelectrophoresis	1522:1549	The SDS-PAGE, immunodiffusion, and linear immunoelectrophoresis analyses showed that in strain KM018 (pRK415-p60025), the wild-type LpsI+ LpsII+ profile was restored.
32607666	12	43	theme	cell-surface	1843:1854	arg1	glycopolymers					1856:1868	the major cell-surface glycopolymers	1833:1868	the major cell-surface glycopolymers	1833:1868	Thus, the AZOBR_p60025-encoded protein significantly affects the composition of the major cell-surface glycopolymers and the single-cell and social motility of azospirilla.
32607666	12	44	theme	major	1837:1841	arg1	glycopolymers					1856:1868	the major cell-surface glycopolymers	1833:1868	the major cell-surface glycopolymers	1833:1868	Thus, the AZOBR_p60025-encoded protein significantly affects the composition of the major cell-surface glycopolymers and the single-cell and social motility of azospirilla.
32607666	7	45	theme	production	972:981	arg1	aspects					940:946	the genetic aspects	928:946	the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla	928:1019	Because of the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla, the aim of this study was to identify and examine in more detail the coding sequence of strain Sp245, inactivated in the mutant.
32607666	7	46	theme	surface	951:957	arg1	production					972:981	surface glycopolymer production	951:981	surface glycopolymer production	951:981	Because of the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla, the aim of this study was to identify and examine in more detail the coding sequence of strain Sp245, inactivated in the mutant.
32607666	1	47	theme	flagella	235:242	arg1	work					209:212	the work	205:212	the work of polar and lateral flagella	205:242	The bacterium Azospirillum brasilense can swim and swarm owing to the work of polar and lateral flagella.
32607666	5	48	theme	Omegon-Km	678:686	arg1	mutagenesis					688:698	random Omegon-Km mutagenesis	671:698	random Omegon-Km mutagenesis	671:698	Previously, using vector pJFF350 for random Omegon-Km mutagenesis, we constructed a mutant of Sp245 named KM018 that still possessed flagella, although paralyzed.
32607666	4	49	theme	identical	605:613	arg1	O-polysaccharides					615:631	identical O-polysaccharides	605:631	identical O-polysaccharides	605:631	The facultative plant endophyte A. brasilense Sp245 produces two antigenically different LPS, LpsI, and LpsII, containing identical O-polysaccharides.
32607666	12	50	theme	AZOBR_p60025-encoded	1763:1782	arg1	protein					1784:1790	the AZOBR_p60025-encoded protein	1759:1790	the AZOBR_p60025-encoded protein	1759:1790	Thus, the AZOBR_p60025-encoded protein significantly affects the composition of the major cell-surface glycopolymers and the single-cell and social motility of azospirilla.
32607666	10	51	theme	strain	1575:1580	arg1	pRK415-p60025					1589:1601	pRK415-p60025	1589:1601	pRK415-p60025	1589:1601	The SDS-PAGE, immunodiffusion, and linear immunoelectrophoresis analyses showed that in strain KM018 (pRK415-p60025), the wild-type LpsI+ LpsII+ profile was restored.
32607666	10	51	theme	strain	1575:1580	arg1	KM018					1582:1586	strain KM018	1575:1586	strain KM018 (pRK415-p60025)	1575:1602	The SDS-PAGE, immunodiffusion, and linear immunoelectrophoresis analyses showed that in strain KM018 (pRK415-p60025), the wild-type LpsI+ LpsII+ profile was restored.
32607666	8	52	theme	unknown	1255:1261	arg1	function					1263:1270	unknown function	1255:1270	unknown function	1255:1270	We found that pJFF350 was integrated into a coding sequence for a putative integral membrane protein of unknown function (AZOBR_p60025) located in the sixth plasmid of Sp245.
32607666	7	53	theme	Sp245	1117:1121	arg1	sequence					1098:1105	the coding sequence	1087:1105	the coding sequence of strain Sp245	1087:1121	Because of the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla, the aim of this study was to identify and examine in more detail the coding sequence of strain Sp245, inactivated in the mutant.
32607666	2	54	theme	Calcofluor-binding	318:335	arg1	polysaccharides					337:351	Calcofluor-binding polysaccharides	318:351	Calcofluor-binding polysaccharides (Cal+ phenotype)	318:368	Its major surface glycopolymers consist of lipopolysaccharides (LPS) and Calcofluor-binding polysaccharides (Cal+ phenotype).
32607666	2	54	theme	Calcofluor-binding	318:335	arg1	phenotype					359:367	Cal+ phenotype	354:367	Cal+ phenotype	354:367	Its major surface glycopolymers consist of lipopolysaccharides (LPS) and Calcofluor-binding polysaccharides (Cal+ phenotype).
32607666	7	55	theme	limited	899:905	arg1	data					920:923	the limited experimental data	895:923	the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla	895:1019	Because of the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla, the aim of this study was to identify and examine in more detail the coding sequence of strain Sp245, inactivated in the mutant.
32607666	7	56	theme	coding	1091:1096	arg1	sequence					1098:1105	the coding sequence	1087:1105	the coding sequence of strain Sp245	1087:1121	Because of the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla, the aim of this study was to identify and examine in more detail the coding sequence of strain Sp245, inactivated in the mutant.
32607666	9	57	theme	mutant	1473:1478	arg1	complementation					1454:1468	the genetic complementation	1442:1468	the genetic complementation of mutant KM018	1442:1484	To clarify the role of the putative protein, we cloned AZOBR_p60025 in the expression vector pRK415 and used it for the genetic complementation of mutant KM018.
32607666	8	58	theme	integral	1226:1233	arg1	protein					1244:1250	a putative integral membrane protein	1215:1250	a putative integral membrane protein of unknown function	1215:1270	We found that pJFF350 was integrated into a coding sequence for a putative integral membrane protein of unknown function (AZOBR_p60025) located in the sixth plasmid of Sp245.
32607666	4	59	theme	plant	499:503	arg1	endophyte					505:513	The facultative plant endophyte A. brasilense Sp245	483:533	The facultative plant endophyte A. brasilense Sp245	483:533	The facultative plant endophyte A. brasilense Sp245 produces two antigenically different LPS, LpsI, and LpsII, containing identical O-polysaccharides.
32607666	10	60	theme	LpsI+	1619:1623	arg1	profile					1632:1638	the wild-type LpsI+ LpsII+ profile	1605:1638	the wild-type LpsI+ LpsII+ profile	1605:1638	The SDS-PAGE, immunodiffusion, and linear immunoelectrophoresis analyses showed that in strain KM018 (pRK415-p60025), the wild-type LpsI+ LpsII+ profile was restored.
32607666	8	61	theme	sixth	1302:1306	arg1	plasmid					1308:1314	the sixth plasmid	1298:1314	the sixth plasmid of Sp245	1298:1323	We found that pJFF350 was integrated into a coding sequence for a putative integral membrane protein of unknown function (AZOBR_p60025) located in the sixth plasmid of Sp245.
32607666	5	62	theme	Sp245	728:732	arg1	mutant					718:723	a mutant	716:723	a mutant of Sp245 named KM018 that still possessed flagella, although paralyzed	716:794	Previously, using vector pJFF350 for random Omegon-Km mutagenesis, we constructed a mutant of Sp245 named KM018 that still possessed flagella, although paralyzed.
32607666	2	63	theme	major	249:253	arg1	glycopolymers					263:275	Its major surface glycopolymers	245:275	Its major surface glycopolymers	245:275	Its major surface glycopolymers consist of lipopolysaccharides (LPS) and Calcofluor-binding polysaccharides (Cal+ phenotype).
32607666	1	64	theme	Azospirillum	153:164	arg1	brasilense					166:175	The bacterium Azospirillum brasilense	139:175	The bacterium Azospirillum brasilense	139:175	The bacterium Azospirillum brasilense can swim and swarm owing to the work of polar and lateral flagella.
32607666	4	65	theme	different	562:570	arg1	LpsI					577:580	LpsI	577:580	LpsI	577:580	The facultative plant endophyte A. brasilense Sp245 produces two antigenically different LPS, LpsI, and LpsII, containing identical O-polysaccharides.
32607666	4	65	theme	different	562:570	arg1	LpsII					587:591	LpsII	587:591	LpsII	587:591	The facultative plant endophyte A. brasilense Sp245 produces two antigenically different LPS, LpsI, and LpsII, containing identical O-polysaccharides.
32607666	4	65	theme	different	562:570	arg1	LPS					572:574	two antigenically different LPS	544:574	two antigenically different LPS	544:574	The facultative plant endophyte A. brasilense Sp245 produces two antigenically different LPS, LpsI, and LpsII, containing identical O-polysaccharides.
32607666	0	66	theme	brasilense	121:130	arg1	Sp245					132:136	Azospirillum brasilense Sp245	108:136	Azospirillum brasilense Sp245	108:136	Plasmid gene for putative integral membrane protein affects formation of lipopolysaccharide and motility in Azospirillum brasilense Sp245.
32607666	12	67	theme	azospirilla	1913:1923	arg1	glycopolymers					1856:1868	the major cell-surface glycopolymers	1833:1868	the major cell-surface glycopolymers	1833:1868	Thus, the AZOBR_p60025-encoded protein significantly affects the composition of the major cell-surface glycopolymers and the single-cell and social motility of azospirilla.
32607666	12	67	theme	azospirilla	1913:1923	arg1	single-cell					1878:1888	single-cell	1878:1888	single-cell	1878:1888	Thus, the AZOBR_p60025-encoded protein significantly affects the composition of the major cell-surface glycopolymers and the single-cell and social motility of azospirilla.
32607666	12	67	theme	azospirilla	1913:1923	arg1	motility					1901:1908	social motility	1894:1908	social motility	1894:1908	Thus, the AZOBR_p60025-encoded protein significantly affects the composition of the major cell-surface glycopolymers and the single-cell and social motility of azospirilla.
32607666	7	68	from	aspects	940:946	arg1	azospirilla					1009:1019	azospirilla	1009:1019	azospirilla	1009:1019	Because of the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla, the aim of this study was to identify and examine in more detail the coding sequence of strain Sp245, inactivated in the mutant.
32607666	11	69	contain	had	1678:1680	arg1	mutant					1671:1676	The complemented mutant	1654:1676	The complemented mutant	1654:1676	The complemented mutant had a Cal+ phenotype and it was capable of swimming and swarming motility.
32607666	11	69	contain	had	1678:1680	arg2	phenotype					1689:1697	a Cal+ phenotype	1682:1697	a Cal+ phenotype	1682:1697	The complemented mutant had a Cal+ phenotype and it was capable of swimming and swarming motility.
32607666	5	70	contain	possessed	757:765	arg2	flagella					767:774	flagella	767:774	flagella	767:774	Previously, using vector pJFF350 for random Omegon-Km mutagenesis, we constructed a mutant of Sp245 named KM018 that still possessed flagella, although paralyzed.
32607666	5	70	contain	possessed	757:765	arg1	mutant					718:723	a mutant	716:723	a mutant of Sp245 named KM018 that still possessed flagella, although paralyzed	716:794	Previously, using vector pJFF350 for random Omegon-Km mutagenesis, we constructed a mutant of Sp245 named KM018 that still possessed flagella, although paralyzed.
32607666	3	71	theme	bacteria	461:468	arg1	interactions					428:439	the interactions	424:439	the interactions of plant-associated bacteria with plants	424:480	Motility and surface glycopolymers are important for the interactions of plant-associated bacteria with plants.
32607666	0	72	theme	putative	17:24	arg1	protein					44:50	putative integral membrane protein	17:50	putative integral membrane protein	17:50	Plasmid gene for putative integral membrane protein affects formation of lipopolysaccharide and motility in Azospirillum brasilense Sp245.
32607666	7	73	theme	flagellar	987:995	arg1	motility					997:1004	flagellar motility	987:1004	flagellar motility	987:1004	Because of the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla, the aim of this study was to identify and examine in more detail the coding sequence of strain Sp245, inactivated in the mutant.
32607666	10	74	theme	SDS-PAGE	1491:1498	arg1	analyses					1551:1558	The SDS-PAGE, immunodiffusion, and linear immunoelectrophoresis analyses	1487:1558	The SDS-PAGE, immunodiffusion, and linear immunoelectrophoresis analyses	1487:1558	The SDS-PAGE, immunodiffusion, and linear immunoelectrophoresis analyses showed that in strain KM018 (pRK415-p60025), the wild-type LpsI+ LpsII+ profile was restored.
32607666	7	75	from	production	972:981	arg1	azospirilla					1009:1019	azospirilla	1009:1019	azospirilla	1009:1019	Because of the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla, the aim of this study was to identify and examine in more detail the coding sequence of strain Sp245, inactivated in the mutant.
32607666	0	76	theme	membrane	35:42	arg1	protein					44:50	putative integral membrane protein	17:50	putative integral membrane protein	17:50	Plasmid gene for putative integral membrane protein affects formation of lipopolysaccharide and motility in Azospirillum brasilense Sp245.
32607666	9	77	theme	putative	1353:1360	arg1	protein					1362:1368	the putative protein	1349:1368	the putative protein	1349:1368	To clarify the role of the putative protein, we cloned AZOBR_p60025 in the expression vector pRK415 and used it for the genetic complementation of mutant KM018.
32607666	11	78	theme	Cal+	1684:1687	arg1	phenotype					1689:1697	a Cal+ phenotype	1682:1697	a Cal+ phenotype	1682:1697	The complemented mutant had a Cal+ phenotype and it was capable of swimming and swarming motility.
32607666	12	79	theme	glycopolymers	1856:1868	arg1	composition					1818:1828	the composition	1814:1828	the composition of the major cell-surface glycopolymers and the single-cell and social motility of azospirilla	1814:1923	Thus, the AZOBR_p60025-encoded protein significantly affects the composition of the major cell-surface glycopolymers and the single-cell and social motility of azospirilla.
32607666	2	80	theme	Cal+	354:357	arg1	polysaccharides					337:351	Calcofluor-binding polysaccharides	318:351	Calcofluor-binding polysaccharides (Cal+ phenotype)	318:368	Its major surface glycopolymers consist of lipopolysaccharides (LPS) and Calcofluor-binding polysaccharides (Cal+ phenotype).
32607666	2	80	theme	Cal+	354:357	arg1	phenotype					359:367	Cal+ phenotype	354:367	Cal+ phenotype	354:367	Its major surface glycopolymers consist of lipopolysaccharides (LPS) and Calcofluor-binding polysaccharides (Cal+ phenotype).
32607666	7	81	theme	glycopolymer	959:970	arg1	production					972:981	surface glycopolymer production	951:981	surface glycopolymer production	951:981	Because of the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla, the aim of this study was to identify and examine in more detail the coding sequence of strain Sp245, inactivated in the mutant.
32607666	10	82	theme	immunodiffusion	1501:1515	arg1	analyses					1551:1558	The SDS-PAGE, immunodiffusion, and linear immunoelectrophoresis analyses	1487:1558	The SDS-PAGE, immunodiffusion, and linear immunoelectrophoresis analyses	1487:1558	The SDS-PAGE, immunodiffusion, and linear immunoelectrophoresis analyses showed that in strain KM018 (pRK415-p60025), the wild-type LpsI+ LpsII+ profile was restored.
32607666	1	83	theme	lateral	227:233	arg1	flagella					235:242	polar and lateral flagella	217:242	polar and lateral flagella	217:242	The bacterium Azospirillum brasilense can swim and swarm owing to the work of polar and lateral flagella.
32607666	9	84	used	used	1430:1433	arg2	we					1371:1372	we	1371:1372	we	1371:1372	To clarify the role of the putative protein, we cloned AZOBR_p60025 in the expression vector pRK415 and used it for the genetic complementation of mutant KM018.
32607666	7	85	from	data	920:923	arg1	aspects					940:946	the genetic aspects	928:946	the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla	928:1019	Because of the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla, the aim of this study was to identify and examine in more detail the coding sequence of strain Sp245, inactivated in the mutant.
32607666	7	86	theme	genetic	932:938	arg1	aspects					940:946	the genetic aspects	928:946	the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla	928:1019	Because of the limited experimental data on the genetic aspects of surface glycopolymer production and flagellar motility in azospirilla, the aim of this study was to identify and examine in more detail the coding sequence of strain Sp245, inactivated in the mutant.
32607666	9	87	theme	expression	1401:1410	arg1	vector					1412:1417	the expression vector pRK415	1397:1424	the expression vector pRK415	1397:1424	To clarify the role of the putative protein, we cloned AZOBR_p60025 in the expression vector pRK415 and used it for the genetic complementation of mutant KM018.
33288163	5	0	theme	underlying	757:766	arg1	mechanisms					768:777	explored underlying mechanisms	748:777	explored underlying mechanisms of AMP	748:784	(AMP) on experimental UC induced by dextran sulfate sodium (DSS) and explored underlying mechanisms of AMP by 16S rDNA-based gut microbiota analysis and untargeted fecal and plasma metabolomics.
33288163	9	1	theme	Untargeted	1174:1183	arg1	fecal					1185:1189	Untargeted fecal and plasma metabolomics	1174:1213	fecal	1185:1189	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	6	2	theme	multiscale	889:898	arg1	network					916:922	a multiscale, multifactorial network	887:922	a multiscale, multifactorial network	887:922	In addition, a multiscale, multifactorial network was constructed to visualize the mechanisms of AMP.
33288163	7	3	from	injury	1064:1069	arg1	mice					1086:1089	DSS treated mice	1074:1089	DSS treated mice	1074:1089	The results showed that AMP significantly increased body weight and ameliorated colonic injury in DSS treated mice.
33288163	7	4	theme	treated	1078:1084	arg1	mice					1086:1089	DSS treated mice	1074:1089	DSS treated mice	1074:1089	The results showed that AMP significantly increased body weight and ameliorated colonic injury in DSS treated mice.
33288163	10	5	dep	SCFAs	1505:1509	arg1	addition					1493:1500	addition	1493:1500	addition	1493:1500	The study demonstrated that, in addition to SCFAs, AMP can extensively modulate the metabolism of gut microbiota and hosts to achieve the therapeutic effects.
33288163	5	6	theme	sodium	731:736	arg1	DSS					739:741	dextran sulfate sodium (DSS)	715:742	dextran sulfate sodium (DSS)	715:742	(AMP) on experimental UC induced by dextran sulfate sodium (DSS) and explored underlying mechanisms of AMP by 16S rDNA-based gut microbiota analysis and untargeted fecal and plasma metabolomics.
33288163	9	7	theme	cadaverine	1399:1408	arg1	production					1385:1394	production	1385:1394	production of cadaverine and other metabolites by hosts	1385:1439	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	9	7	theme	cadaverine	1399:1408	arg1	ability					1309:1315	the ability	1305:1315	the ability to digest food nutrients	1305:1340	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	9	7	theme	cadaverine	1399:1408	arg1	metabolism					1343:1352	metabolism	1343:1352	metabolism of amino acids and bile acids	1343:1382	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	9	7	theme	cadaverine	1399:1408	arg1	production					1257:1266	the production	1253:1266	the production of SCFAs by gut microbiota	1253:1293	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	9	7	theme	cadaverine	1399:1408	arg1	microbiota					1449:1458	gut microbiota	1445:1458	gut microbiota	1445:1458	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	5	8	dep	UC	701:702	arg1	AMP					680:682	AMP	680:682	AMP	680:682	(AMP) on experimental UC induced by dextran sulfate sodium (DSS) and explored underlying mechanisms of AMP by 16S rDNA-based gut microbiota analysis and untargeted fecal and plasma metabolomics.
33288163	2	9	theme	metabolites	179:189	arg1	Turbulence					150:159	Turbulence	150:159	Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids	150:254	Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids is an important factor in the development of diseases.
33288163	2	9	theme	metabolites	179:189	arg1	factor					272:277	an important factor	259:277	an important factor in the development of diseases	259:308	Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids is an important factor in the development of diseases.
33288163	9	10	theme	plasma	1195:1200	arg1	metabolomics					1202:1213	Untargeted fecal and plasma metabolomics	1174:1213	metabolomics	1202:1213	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	3	11	theme	gut	556:558	arg1	microbiota					560:569	gut microbiota	556:569	gut microbiota	556:569	Many polysaccharides are effective on diseases including ulcerative colitis (UC), yet most studies investigating the mechanisms of polysaccharides mainly focused on their effects on gut microbiota composition and SCFAs, and other metabolites of gut microbiota are often neglected.
33288163	4	12	from	Koidz	672:676	arg1	polysaccharides					625:639	polysaccharides	625:639	polysaccharides from Atractylodes macrocephala Koidz	625:676	Here, we examined the effects of polysaccharides from Atractylodes macrocephala Koidz.
33288163	4	12	from	Koidz	672:676	arg1	effects					614:620	the effects	610:620	the effects of polysaccharides from Atractylodes macrocephala Koidz	610:676	Here, we examined the effects of polysaccharides from Atractylodes macrocephala Koidz.
33288163	9	13	theme	acids	1378:1382	arg1	production					1385:1394	production	1385:1394	production of cadaverine and other metabolites by hosts	1385:1439	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	9	13	theme	acids	1378:1382	arg1	ability					1309:1315	the ability	1305:1315	the ability to digest food nutrients	1305:1340	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	9	13	theme	acids	1378:1382	arg1	metabolism					1343:1352	metabolism	1343:1352	metabolism of amino acids and bile acids	1343:1382	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	9	13	theme	acids	1378:1382	arg1	production					1257:1266	the production	1253:1266	the production of SCFAs by gut microbiota	1253:1293	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	9	13	theme	acids	1378:1382	arg1	microbiota					1449:1458	gut microbiota	1445:1458	gut microbiota	1445:1458	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	1	14	theme	host	133:136	arg1	metabolism					138:147	host metabolism	133:147	host metabolism	133:147	Ameliorate ulcerative colitis via extensive modification of gut microbiota and host metabolism.
33288163	5	15	theme	untargeted	832:841	arg1	fecal					843:847	untargeted fecal and plasma metabolomics	832:871	fecal	843:847	(AMP) on experimental UC induced by dextran sulfate sodium (DSS) and explored underlying mechanisms of AMP by 16S rDNA-based gut microbiota analysis and untargeted fecal and plasma metabolomics.
33288163	1	16	theme	extensive	88:96	arg1	modification					98:109	extensive modification	88:109	extensive modification of gut microbiota and host metabolism	88:147	Ameliorate ulcerative colitis via extensive modification of gut microbiota and host metabolism.
33288163	3	17	theme	polysaccharides	442:456	arg1	mechanisms					428:437	the mechanisms	424:437	the mechanisms of polysaccharides mainly focused on their effects on gut microbiota composition and SCFAs	424:528	Many polysaccharides are effective on diseases including ulcerative colitis (UC), yet most studies investigating the mechanisms of polysaccharides mainly focused on their effects on gut microbiota composition and SCFAs, and other metabolites of gut microbiota are often neglected.
33288163	3	18	theme	ulcerative	368:377	arg1	UC					388:389	UC	388:389	UC	388:389	Many polysaccharides are effective on diseases including ulcerative colitis (UC), yet most studies investigating the mechanisms of polysaccharides mainly focused on their effects on gut microbiota composition and SCFAs, and other metabolites of gut microbiota are often neglected.
33288163	3	18	theme	ulcerative	368:377	arg1	colitis					379:385	ulcerative colitis	368:385	ulcerative colitis (UC)	368:390	Many polysaccharides are effective on diseases including ulcerative colitis (UC), yet most studies investigating the mechanisms of polysaccharides mainly focused on their effects on gut microbiota composition and SCFAs, and other metabolites of gut microbiota are often neglected.
33288163	2	19	theme	gut	164:166	arg1	acids					250:254	secondary bile acids	235:254	secondary bile acids	235:254	Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids is an important factor in the development of diseases.
33288163	2	19	theme	gut	164:166	arg1	acids					217:221	short-chain fatty acids	199:221	short-chain fatty acids (SCFAs)	199:229	Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids is an important factor in the development of diseases.
33288163	2	19	theme	gut	164:166	arg1	metabolites					179:189	gut microbiota metabolites	164:189	gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids	164:254	Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids is an important factor in the development of diseases.
33288163	10	20	theme	microbiota	1563:1572	arg1	metabolism					1545:1554	the metabolism	1541:1554	the metabolism of gut microbiota	1541:1572	The study demonstrated that, in addition to SCFAs, AMP can extensively modulate the metabolism of gut microbiota and hosts to achieve the therapeutic effects.
33288163	9	21	theme	metabolites	1420:1430	arg1	production					1385:1394	production	1385:1394	production of cadaverine and other metabolites by hosts	1385:1439	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	9	21	theme	metabolites	1420:1430	arg1	ability					1309:1315	the ability	1305:1315	the ability to digest food nutrients	1305:1340	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	9	21	theme	metabolites	1420:1430	arg1	metabolism					1343:1352	metabolism	1343:1352	metabolism of amino acids and bile acids	1343:1382	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	9	21	theme	metabolites	1420:1430	arg1	production					1257:1266	the production	1253:1266	the production of SCFAs by gut microbiota	1253:1293	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	9	21	theme	metabolites	1420:1430	arg1	microbiota					1449:1458	gut microbiota	1445:1458	gut microbiota	1445:1458	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	5	22	theme	rDNA-based	793:802	arg1	analysis					819:826	rDNA-based gut microbiota analysis	793:826	rDNA-based gut microbiota analysis	793:826	(AMP) on experimental UC induced by dextran sulfate sodium (DSS) and explored underlying mechanisms of AMP by 16S rDNA-based gut microbiota analysis and untargeted fecal and plasma metabolomics.
33288163	0	23	from	Koidz	47:51	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from Atractylodes macrocephala Koidz.	0:52	Polysaccharides from Atractylodes macrocephala Koidz.
33288163	2	24	theme	microbiota	168:177	arg1	acids					250:254	secondary bile acids	235:254	secondary bile acids	235:254	Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids is an important factor in the development of diseases.
33288163	2	24	theme	microbiota	168:177	arg1	acids					217:221	short-chain fatty acids	199:221	short-chain fatty acids (SCFAs)	199:229	Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids is an important factor in the development of diseases.
33288163	2	24	theme	microbiota	168:177	arg1	metabolites					179:189	gut microbiota metabolites	164:189	gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids	164:254	Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids is an important factor in the development of diseases.
33288163	5	25	theme	gut	804:806	arg1	analysis					819:826	rDNA-based gut microbiota analysis	793:826	rDNA-based gut microbiota analysis	793:826	(AMP) on experimental UC induced by dextran sulfate sodium (DSS) and explored underlying mechanisms of AMP by 16S rDNA-based gut microbiota analysis and untargeted fecal and plasma metabolomics.
33288163	1	26	theme	gut	114:116	arg1	microbiota					118:127	gut microbiota	114:127	gut microbiota	114:127	Ameliorate ulcerative colitis via extensive modification of gut microbiota and host metabolism.
33288163	10	27	theme	therapeutic	1599:1609	arg1	effects					1611:1617	the therapeutic effects	1595:1617	the therapeutic effects	1595:1617	The study demonstrated that, in addition to SCFAs, AMP can extensively modulate the metabolism of gut microbiota and hosts to achieve the therapeutic effects.
33288163	5	28	theme	microbiota	808:817	arg1	analysis					819:826	rDNA-based gut microbiota analysis	793:826	rDNA-based gut microbiota analysis	793:826	(AMP) on experimental UC induced by dextran sulfate sodium (DSS) and explored underlying mechanisms of AMP by 16S rDNA-based gut microbiota analysis and untargeted fecal and plasma metabolomics.
33288163	6	29	theme	AMP	971:973	arg1	mechanisms					957:966	the mechanisms	953:966	the mechanisms of AMP	953:973	In addition, a multiscale, multifactorial network was constructed to visualize the mechanisms of AMP.
33288163	7	30	theme	DSS	1074:1076	arg1	mice					1086:1089	DSS treated mice	1074:1089	DSS treated mice	1074:1089	The results showed that AMP significantly increased body weight and ameliorated colonic injury in DSS treated mice.
33288163	9	31	theme	other	1414:1418	arg1	metabolites					1420:1430	other metabolites	1414:1430	other metabolites	1414:1430	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	3	32	theme	microbiota	560:569	arg1	studies					402:408	most studies	397:408	most studies investigating the mechanisms of polysaccharides mainly focused on their effects on gut microbiota composition and SCFAs	397:528	Many polysaccharides are effective on diseases including ulcerative colitis (UC), yet most studies investigating the mechanisms of polysaccharides mainly focused on their effects on gut microbiota composition and SCFAs, and other metabolites of gut microbiota are often neglected.
33288163	3	32	theme	microbiota	560:569	arg1	metabolites					541:551	other metabolites	535:551	other metabolites of gut microbiota	535:569	Many polysaccharides are effective on diseases including ulcerative colitis (UC), yet most studies investigating the mechanisms of polysaccharides mainly focused on their effects on gut microbiota composition and SCFAs, and other metabolites of gut microbiota are often neglected.
33288163	9	33	theme	gut	1445:1447	arg1	microbiota					1449:1458	gut microbiota	1445:1458	gut microbiota	1445:1458	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	3	34	theme	other	535:539	arg1	metabolites					541:551	other metabolites	535:551	other metabolites of gut microbiota	535:569	Many polysaccharides are effective on diseases including ulcerative colitis (UC), yet most studies investigating the mechanisms of polysaccharides mainly focused on their effects on gut microbiota composition and SCFAs, and other metabolites of gut microbiota are often neglected.
33288163	2	35	theme	fatty	211:215	arg1	SCFAs					224:228	SCFAs	224:228	SCFAs	224:228	Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids is an important factor in the development of diseases.
33288163	2	35	theme	fatty	211:215	arg1	acids					217:221	short-chain fatty acids	199:221	short-chain fatty acids (SCFAs)	199:229	Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids is an important factor in the development of diseases.
33288163	2	36	theme	diseases	301:308	arg1	development					286:296	the development	282:296	the development of diseases	282:308	Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids is an important factor in the development of diseases.
33288163	2	37	from	factor	272:277	arg1	development					286:296	the development	282:296	the development of diseases	282:308	Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids is an important factor in the development of diseases.
33288163	4	38	theme	polysaccharides	625:639	arg1	effects					614:620	the effects	610:620	the effects of polysaccharides from Atractylodes macrocephala Koidz	610:676	Here, we examined the effects of polysaccharides from Atractylodes macrocephala Koidz.
33288163	5	39	theme	dextran	715:721	arg1	DSS					739:741	dextran sulfate sodium (DSS)	715:742	dextran sulfate sodium (DSS)	715:742	(AMP) on experimental UC induced by dextran sulfate sodium (DSS) and explored underlying mechanisms of AMP by 16S rDNA-based gut microbiota analysis and untargeted fecal and plasma metabolomics.
33288163	2	40	theme	short-chain	199:209	arg1	SCFAs					224:228	SCFAs	224:228	SCFAs	224:228	Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids is an important factor in the development of diseases.
33288163	2	40	theme	short-chain	199:209	arg1	acids					217:221	short-chain fatty acids	199:221	short-chain fatty acids (SCFAs)	199:229	Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids is an important factor in the development of diseases.
33288163	10	41	theme	gut	1559:1561	arg1	microbiota					1563:1572	gut microbiota	1559:1572	gut microbiota	1559:1572	The study demonstrated that, in addition to SCFAs, AMP can extensively modulate the metabolism of gut microbiota and hosts to achieve the therapeutic effects.
33288163	9	42	theme	food	1327:1330	arg1	nutrients					1332:1340	food nutrients	1327:1340	food nutrients	1327:1340	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	5	43	theme	plasma	853:858	arg1	metabolomics					860:871	untargeted fecal and plasma metabolomics	832:871	metabolomics	860:871	(AMP) on experimental UC induced by dextran sulfate sodium (DSS) and explored underlying mechanisms of AMP by 16S rDNA-based gut microbiota analysis and untargeted fecal and plasma metabolomics.
33288163	9	44	dep	ability	1309:1315	arg1	digest					1320:1325	digest	1320:1325	to digest food nutrients	1317:1340	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	5	45	theme	sulfate	723:729	arg1	DSS					739:741	dextran sulfate sodium (DSS)	715:742	dextran sulfate sodium (DSS)	715:742	(AMP) on experimental UC induced by dextran sulfate sodium (DSS) and explored underlying mechanisms of AMP by 16S rDNA-based gut microbiota analysis and untargeted fecal and plasma metabolomics.
33288163	2	46	theme	bile	245:248	arg1	acids					250:254	secondary bile acids	235:254	secondary bile acids	235:254	Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids is an important factor in the development of diseases.
33288163	3	47	theme	most	397:400	arg1	studies					402:408	most studies	397:408	most studies investigating the mechanisms of polysaccharides mainly focused on their effects on gut microbiota composition and SCFAs	397:528	Many polysaccharides are effective on diseases including ulcerative colitis (UC), yet most studies investigating the mechanisms of polysaccharides mainly focused on their effects on gut microbiota composition and SCFAs, and other metabolites of gut microbiota are often neglected.
33288163	3	48	theme	Many	311:314	arg1	polysaccharides					316:330	Many polysaccharides	311:330	Many polysaccharides	311:330	Many polysaccharides are effective on diseases including ulcerative colitis (UC), yet most studies investigating the mechanisms of polysaccharides mainly focused on their effects on gut microbiota composition and SCFAs, and other metabolites of gut microbiota are often neglected.
33288163	5	49	theme	explored	748:755	arg1	mechanisms					768:777	explored underlying mechanisms	748:777	explored underlying mechanisms of AMP	748:784	(AMP) on experimental UC induced by dextran sulfate sodium (DSS) and explored underlying mechanisms of AMP by 16S rDNA-based gut microbiota analysis and untargeted fecal and plasma metabolomics.
33288163	2	50	theme	secondary	235:243	arg1	acids					250:254	secondary bile acids	235:254	secondary bile acids	235:254	Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids is an important factor in the development of diseases.
33288163	7	51	theme	body	1028:1031	arg1	weight					1033:1038	body weight	1028:1038	body weight	1028:1038	The results showed that AMP significantly increased body weight and ameliorated colonic injury in DSS treated mice.
33288163	3	52	theme	microbiota	497:506	arg1	composition					508:518	gut microbiota composition	493:518	gut microbiota composition	493:518	Many polysaccharides are effective on diseases including ulcerative colitis (UC), yet most studies investigating the mechanisms of polysaccharides mainly focused on their effects on gut microbiota composition and SCFAs, and other metabolites of gut microbiota are often neglected.
33288163	9	53	theme	amino	1357:1361	arg1	acids					1363:1367	amino acids	1357:1367	amino acids	1357:1367	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	7	54	theme	colonic	1056:1062	arg1	injury					1064:1069	colonic injury	1056:1069	colonic injury in DSS treated mice	1056:1089	The results showed that AMP significantly increased body weight and ameliorated colonic injury in DSS treated mice.
33288163	1	55	theme	metabolism	138:147	arg1	modification					98:109	extensive modification	88:109	extensive modification of gut microbiota and host metabolism	88:147	Ameliorate ulcerative colitis via extensive modification of gut microbiota and host metabolism.
33288163	9	56	theme	acids	1363:1367	arg1	production					1385:1394	production	1385:1394	production of cadaverine and other metabolites by hosts	1385:1439	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	9	56	theme	acids	1363:1367	arg1	ability					1309:1315	the ability	1305:1315	the ability to digest food nutrients	1305:1340	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	9	56	theme	acids	1363:1367	arg1	metabolism					1343:1352	metabolism	1343:1352	metabolism of amino acids and bile acids	1343:1382	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	9	56	theme	acids	1363:1367	arg1	production					1257:1266	the production	1253:1266	the production of SCFAs by gut microbiota	1253:1293	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	9	56	theme	acids	1363:1367	arg1	microbiota					1449:1458	gut microbiota	1445:1458	gut microbiota	1445:1458	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	5	57	theme	experimental	688:699	arg1	UC					701:702	experimental UC	688:702	experimental UC induced by dextran sulfate sodium (DSS) and explored underlying mechanisms of AMP by 16S rDNA-based gut microbiota analysis and untargeted fecal and plasma metabolomics	688:871	(AMP) on experimental UC induced by dextran sulfate sodium (DSS) and explored underlying mechanisms of AMP by 16S rDNA-based gut microbiota analysis and untargeted fecal and plasma metabolomics.
33288163	5	58	theme	AMP	782:784	arg1	DSS					739:741	dextran sulfate sodium (DSS)	715:742	dextran sulfate sodium (DSS)	715:742	(AMP) on experimental UC induced by dextran sulfate sodium (DSS) and explored underlying mechanisms of AMP by 16S rDNA-based gut microbiota analysis and untargeted fecal and plasma metabolomics.
33288163	5	58	theme	AMP	782:784	arg1	mechanisms					768:777	explored underlying mechanisms	748:777	explored underlying mechanisms of AMP	748:784	(AMP) on experimental UC induced by dextran sulfate sodium (DSS) and explored underlying mechanisms of AMP by 16S rDNA-based gut microbiota analysis and untargeted fecal and plasma metabolomics.
33288163	3	59	from	effects	482:488	arg1	SCFAs					524:528	SCFAs	524:528	SCFAs	524:528	Many polysaccharides are effective on diseases including ulcerative colitis (UC), yet most studies investigating the mechanisms of polysaccharides mainly focused on their effects on gut microbiota composition and SCFAs, and other metabolites of gut microbiota are often neglected.
33288163	3	59	from	effects	482:488	arg1	composition					508:518	gut microbiota composition	493:518	gut microbiota composition	493:518	Many polysaccharides are effective on diseases including ulcerative colitis (UC), yet most studies investigating the mechanisms of polysaccharides mainly focused on their effects on gut microbiota composition and SCFAs, and other metabolites of gut microbiota are often neglected.
33288163	1	60	theme	microbiota	118:127	arg1	modification					98:109	extensive modification	88:109	extensive modification of gut microbiota and host metabolism	88:147	Ameliorate ulcerative colitis via extensive modification of gut microbiota and host metabolism.
33288163	11	61	theme	new	1636:1638	arg1	mechanisms					1640:1649	new mechanisms	1636:1649	new mechanisms of polysaccharides	1636:1668	This study adds new mechanisms of polysaccharides in treating diseases.
33288163	9	62	theme	SCFAs	1271:1275	arg1	production					1385:1394	production	1385:1394	production of cadaverine and other metabolites by hosts	1385:1439	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	9	62	theme	SCFAs	1271:1275	arg1	ability					1309:1315	the ability	1305:1315	the ability to digest food nutrients	1305:1340	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	9	62	theme	SCFAs	1271:1275	arg1	metabolism					1343:1352	metabolism	1343:1352	metabolism of amino acids and bile acids	1343:1382	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	9	62	theme	SCFAs	1271:1275	arg1	production					1257:1266	the production	1253:1266	the production of SCFAs by gut microbiota	1253:1293	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	9	62	theme	SCFAs	1271:1275	arg1	microbiota					1449:1458	gut microbiota	1445:1458	gut microbiota	1445:1458	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	2	63	theme	important	262:270	arg1	Turbulence					150:159	Turbulence	150:159	Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids	150:254	Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids is an important factor in the development of diseases.
33288163	2	63	theme	important	262:270	arg1	factor					272:277	an important factor	259:277	an important factor in the development of diseases	259:308	Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids is an important factor in the development of diseases.
33288163	8	64	theme	perturbed	1121:1129	arg1	composition					1146:1156	the perturbed gut microbiota composition	1117:1156	the perturbed gut microbiota composition induced by DSS	1117:1171	AMP also partly restored the perturbed gut microbiota composition induced by DSS.
33288163	3	65	theme	gut	493:495	arg1	composition					508:518	gut microbiota composition	493:518	gut microbiota composition	493:518	Many polysaccharides are effective on diseases including ulcerative colitis (UC), yet most studies investigating the mechanisms of polysaccharides mainly focused on their effects on gut microbiota composition and SCFAs, and other metabolites of gut microbiota are often neglected.
33288163	8	66	theme	gut	1131:1133	arg1	composition					1146:1156	the perturbed gut microbiota composition	1117:1156	the perturbed gut microbiota composition induced by DSS	1117:1171	AMP also partly restored the perturbed gut microbiota composition induced by DSS.
33288163	9	67	theme	bile	1373:1376	arg1	acids					1378:1382	bile acids	1373:1382	bile acids	1373:1382	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	8	68	theme	microbiota	1135:1144	arg1	composition					1146:1156	the perturbed gut microbiota composition	1117:1156	the perturbed gut microbiota composition induced by DSS	1117:1171	AMP also partly restored the perturbed gut microbiota composition induced by DSS.
33288163	6	69	dep	multiscale	889:898	arg1	multifactorial					901:914	multifactorial	901:914	multifactorial	901:914	In addition, a multiscale, multifactorial network was constructed to visualize the mechanisms of AMP.
33288163	4	70	from	effects	614:620	arg1	Koidz					672:676	Koidz	672:676	Koidz	672:676	Here, we examined the effects of polysaccharides from Atractylodes macrocephala Koidz.
33288163	9	71	theme	gut	1280:1282	arg1	microbiota					1284:1293	gut microbiota	1280:1293	gut microbiota	1280:1293	Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota.
33288163	1	72	theme	ulcerative	65:74	arg1	colitis					76:82	ulcerative colitis	65:82	ulcerative colitis	65:82	Ameliorate ulcerative colitis via extensive modification of gut microbiota and host metabolism.
33288163	11	73	theme	polysaccharides	1654:1668	arg1	mechanisms					1640:1649	new mechanisms	1636:1649	new mechanisms of polysaccharides	1636:1668	This study adds new mechanisms of polysaccharides in treating diseases.
35074114	1	0	theme	biomimetic	179:188	arg1	grafts					199:204	biomimetic vascular grafts	179:204	biomimetic vascular grafts	179:204	To achieve long-term patent small-diameter (<6 mm) vascular implants, biomimetic vascular grafts have gained much attention in promoting in situ blood vessel regeneration.
35074114	5	1	theme	inner	794:798	arg1	surface					800:806	a dense inner surface	786:806	a dense inner surface	786:806	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	5	2	theme	BC/PS	767:771	arg1	grafts					773:778	the BC/PS grafts	763:778	the BC/PS grafts	763:778	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	5	3	theme	in	925:926	arg1	assessment					933:942	in vivo assessment	925:942	in vivo assessment on rabbits	925:953	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	1	4	theme	vascular	190:197	arg1	grafts					199:204	biomimetic vascular grafts	179:204	biomimetic vascular grafts	179:204	To achieve long-term patent small-diameter (<6 mm) vascular implants, biomimetic vascular grafts have gained much attention in promoting in situ blood vessel regeneration.
35074114	4	5	theme	water	621:625	arg1	content					627:633	water content	621:633	water content	621:633	The composites displayed a morphology, water content, thermal properties, mechanical properties, and biocompatibility appropriate for vascular tissue engineering.
35074114	6	6	theme	hold	1164:1167	arg1	grafts					1221:1226	artificial small-diameter vascular grafts	1186:1226	artificial small-diameter vascular grafts	1186:1226	Collectively, these findings demonstrate that hierarchical-structured BC/PS tubes hold great promise as artificial small-diameter vascular grafts.
35074114	6	6	theme	hold	1164:1167	arg1	promise					1175:1181	hold great promise	1164:1181	hold great promise	1164:1181	Collectively, these findings demonstrate that hierarchical-structured BC/PS tubes hold great promise as artificial small-diameter vascular grafts.
35074114	2	7	theme	cellulose/potato	330:345	arg1	BC/PS					355:359	BC/PS	355:359	BC/PS	355:359	In this study, hierarchical-structured bacterial cellulose/potato starch (BC/PS) composites were biosynthesized by the addition of swollen PS.
35074114	2	7	theme	cellulose/potato	330:345	arg1	starch					347:352	bacterial cellulose/potato starch	320:352	hierarchical-structured bacterial cellulose/potato starch (BC/PS) composites	296:371	In this study, hierarchical-structured bacterial cellulose/potato starch (BC/PS) composites were biosynthesized by the addition of swollen PS.
35074114	1	8	theme	small-diameter	137:150	arg1	implants					169:176	long-term patent small-diameter (<6 mm) vascular implants	120:176	long-term patent small-diameter (<6 mm) vascular implants	120:176	To achieve long-term patent small-diameter (<6 mm) vascular implants, biomimetic vascular grafts have gained much attention in promoting in situ blood vessel regeneration.
35074114	4	9	theme	thermal	636:642	arg1	properties					644:653	thermal properties	636:653	thermal properties	636:653	The composites displayed a morphology, water content, thermal properties, mechanical properties, and biocompatibility appropriate for vascular tissue engineering.
35074114	2	10	theme	bacterial	320:328	arg1	BC/PS					355:359	BC/PS	355:359	BC/PS	355:359	In this study, hierarchical-structured bacterial cellulose/potato starch (BC/PS) composites were biosynthesized by the addition of swollen PS.
35074114	2	10	theme	bacterial	320:328	arg1	starch					347:352	bacterial cellulose/potato starch	320:352	hierarchical-structured bacterial cellulose/potato starch (BC/PS) composites	296:371	In this study, hierarchical-structured bacterial cellulose/potato starch (BC/PS) composites were biosynthesized by the addition of swollen PS.
35074114	5	11	theme	endothelium	970:980	arg1	monolayer					982:990	complete endothelium monolayer	961:990	complete endothelium monolayer	961:990	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	5	12	theme	dense	788:792	arg1	surface					800:806	a dense inner surface	786:806	a dense inner surface	786:806	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	5	13	from	assessment	933:942	arg1	rabbits					947:953	rabbits	947:953	rabbits	947:953	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	2	14	theme	hierarchical-structured	296:318	arg1	composites					362:371	hierarchical-structured bacterial cellulose/potato starch (BC/PS) composites	296:371	hierarchical-structured bacterial cellulose/potato starch (BC/PS) composites	296:371	In this study, hierarchical-structured bacterial cellulose/potato starch (BC/PS) composites were biosynthesized by the addition of swollen PS.
35074114	4	15	theme	vascular	716:723	arg1	engineering					732:742	vascular tissue engineering	716:742	vascular tissue engineering	716:742	The composites displayed a morphology, water content, thermal properties, mechanical properties, and biocompatibility appropriate for vascular tissue engineering.
35074114	3	16	theme	physicochemical	446:460	arg1	properties					462:471	the physicochemical properties	442:471	the physicochemical properties of BC/PS composites	442:491	Investigations on the physicochemical properties of BC/PS composites showed that the properties could be improved and tailored by the addition of swollen PS.
35074114	3	17	from	Investigations	424:437	arg1	properties					462:471	the physicochemical properties	442:471	the physicochemical properties of BC/PS composites	442:491	Investigations on the physicochemical properties of BC/PS composites showed that the properties could be improved and tailored by the addition of swollen PS.
35074114	3	18	theme	composites	482:491	arg1	properties					462:471	the physicochemical properties	442:471	the physicochemical properties of BC/PS composites	442:491	Investigations on the physicochemical properties of BC/PS composites showed that the properties could be improved and tailored by the addition of swollen PS.
35074114	5	19	theme	75	865:866	arg1	%					867:867	%	867:867	%	867:867	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	6	20	theme	hierarchical-structured	1128:1150	arg1	BC/PS					1152:1156	hierarchical-structured BC/PS	1128:1156	hierarchical-structured BC/PS	1128:1156	Collectively, these findings demonstrate that hierarchical-structured BC/PS tubes hold great promise as artificial small-diameter vascular grafts.
35074114	0	21	theme	bacterial	24:32	arg1	starch					51:56	Hierarchical-structured bacterial cellulose/potato starch	0:56	Hierarchical-structured bacterial cellulose/potato starch	0:56	Hierarchical-structured bacterial cellulose/potato starch tubes as potential small-diameter vascular grafts.
35074114	5	22	theme	circumferential	814:828	arg1	layer					848:852	a circumferential macroporous outer layer	812:852	a circumferential macroporous outer layer	812:852	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	3	23	theme	BC/PS	476:480	arg1	composites					482:491	BC/PS composites	476:491	BC/PS composites	476:491	Investigations on the physicochemical properties of BC/PS composites showed that the properties could be improved and tailored by the addition of swollen PS.
35074114	0	24	theme	Hierarchical-structured	0:22	arg1	starch					51:56	Hierarchical-structured bacterial cellulose/potato starch	0:56	Hierarchical-structured bacterial cellulose/potato starch	0:56	Hierarchical-structured bacterial cellulose/potato starch tubes as potential small-diameter vascular grafts.
35074114	1	25	theme	long-term	120:128	arg1	implants					169:176	long-term patent small-diameter (<6 mm) vascular implants	120:176	long-term patent small-diameter (<6 mm) vascular implants	120:176	To achieve long-term patent small-diameter (<6 mm) vascular implants, biomimetic vascular grafts have gained much attention in promoting in situ blood vessel regeneration.
35074114	2	26	theme	PS	420:421	arg1	addition					400:407	the addition	396:407	the addition of swollen PS	396:421	In this study, hierarchical-structured bacterial cellulose/potato starch (BC/PS) composites were biosynthesized by the addition of swollen PS.
35074114	5	27	theme	%	867:867	arg1	patency					869:875	75% patency	865:875	75% patency	865:875	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	1	28	theme	patent	130:135	arg1	implants					169:176	long-term patent small-diameter (<6 mm) vascular implants	120:176	long-term patent small-diameter (<6 mm) vascular implants	120:176	To achieve long-term patent small-diameter (<6 mm) vascular implants, biomimetic vascular grafts have gained much attention in promoting in situ blood vessel regeneration.
35074114	5	29	theme	muscle	1010:1015	arg1	cells					1017:1021	organized smooth muscle cells	993:1021	organized smooth muscle cells	993:1021	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	0	30	theme	cellulose/potato	34:49	arg1	starch					51:56	Hierarchical-structured bacterial cellulose/potato starch	0:56	Hierarchical-structured bacterial cellulose/potato starch	0:56	Hierarchical-structured bacterial cellulose/potato starch tubes as potential small-diameter vascular grafts.
35074114	3	31	theme	PS	578:579	arg1	addition					558:565	the addition	554:565	the addition of swollen PS	554:579	Investigations on the physicochemical properties of BC/PS composites showed that the properties could be improved and tailored by the addition of swollen PS.
35074114	2	32	theme	starch	347:352	arg1	composites					362:371	hierarchical-structured bacterial cellulose/potato starch (BC/PS) composites	296:371	hierarchical-structured bacterial cellulose/potato starch (BC/PS) composites	296:371	In this study, hierarchical-structured bacterial cellulose/potato starch (BC/PS) composites were biosynthesized by the addition of swollen PS.
35074114	5	33	theme	macroporous	830:840	arg1	layer					848:852	a circumferential macroporous outer layer	812:852	a circumferential macroporous outer layer	812:852	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	6	34	theme	small-diameter	1197:1210	arg1	grafts					1221:1226	artificial small-diameter vascular grafts	1186:1226	artificial small-diameter vascular grafts	1186:1226	Collectively, these findings demonstrate that hierarchical-structured BC/PS tubes hold great promise as artificial small-diameter vascular grafts.
35074114	6	34	theme	small-diameter	1197:1210	arg1	promise					1175:1181	hold great promise	1164:1181	hold great promise	1164:1181	Collectively, these findings demonstrate that hierarchical-structured BC/PS tubes hold great promise as artificial small-diameter vascular grafts.
35074114	5	35	theme	rapid	890:894	arg1	regeneration					909:920	rapid blood vessel regeneration	890:920	rapid blood vessel regeneration	890:920	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	5	36	theme	deposited	1050:1058	arg1	matrix					1074:1079	deposited extracellular matrix	1050:1079	deposited extracellular matrix	1050:1079	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	1	37	theme	much	218:221	arg1	attention					223:231	much attention	218:231	much attention	218:231	To achieve long-term patent small-diameter (<6 mm) vascular implants, biomimetic vascular grafts have gained much attention in promoting in situ blood vessel regeneration.
35074114	4	38	theme	appropriate	700:710	arg1	morphology					609:618	a morphology	607:618	a morphology	607:618	The composites displayed a morphology, water content, thermal properties, mechanical properties, and biocompatibility appropriate for vascular tissue engineering.
35074114	5	39	theme	vessel	902:907	arg1	regeneration					909:920	rapid blood vessel regeneration	890:920	rapid blood vessel regeneration	890:920	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	5	40	theme	outer	842:846	arg1	layer					848:852	a circumferential macroporous outer layer	812:852	a circumferential macroporous outer layer	812:852	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	5	41	theme	extracellular	1060:1072	arg1	matrix					1074:1079	deposited extracellular matrix	1050:1079	deposited extracellular matrix	1050:1079	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	1	42	theme	in	246:247	arg1	regeneration					267:278	in situ blood vessel regeneration	246:278	in situ blood vessel regeneration	246:278	To achieve long-term patent small-diameter (<6 mm) vascular implants, biomimetic vascular grafts have gained much attention in promoting in situ blood vessel regeneration.
35074114	0	43	theme	small-diameter	77:90	arg1	grafts					101:106	potential small-diameter vascular grafts	67:106	potential small-diameter vascular grafts	67:106	Hierarchical-structured bacterial cellulose/potato starch tubes as potential small-diameter vascular grafts.
35074114	5	44	contain	possessed	855:863	arg2	patency					869:875	75% patency	865:875	75% patency	865:875	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	5	44	contain	possessed	855:863	arg2	cells					1017:1021	organized smooth muscle cells	993:1021	organized smooth muscle cells	993:1021	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	5	44	contain	possessed	855:863	arg2	matrix					1074:1079	deposited extracellular matrix	1050:1079	deposited extracellular matrix	1050:1079	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	5	44	contain	possessed	855:863	arg1	grafts					773:778	the BC/PS grafts	763:778	the BC/PS grafts	763:778	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	5	44	contain	possessed	855:863	arg2	capillaries					1033:1043	rich new capillaries	1024:1043	rich new capillaries	1024:1043	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	6	45	theme	artificial	1186:1195	arg1	grafts					1221:1226	artificial small-diameter vascular grafts	1186:1226	artificial small-diameter vascular grafts	1186:1226	Collectively, these findings demonstrate that hierarchical-structured BC/PS tubes hold great promise as artificial small-diameter vascular grafts.
35074114	6	45	theme	artificial	1186:1195	arg1	promise					1175:1181	hold great promise	1164:1181	hold great promise	1164:1181	Collectively, these findings demonstrate that hierarchical-structured BC/PS tubes hold great promise as artificial small-diameter vascular grafts.
35074114	6	46	theme	vascular	1212:1219	arg1	grafts					1221:1226	artificial small-diameter vascular grafts	1186:1226	artificial small-diameter vascular grafts	1186:1226	Collectively, these findings demonstrate that hierarchical-structured BC/PS tubes hold great promise as artificial small-diameter vascular grafts.
35074114	6	46	theme	vascular	1212:1219	arg1	promise					1175:1181	hold great promise	1164:1181	hold great promise	1164:1181	Collectively, these findings demonstrate that hierarchical-structured BC/PS tubes hold great promise as artificial small-diameter vascular grafts.
35074114	0	47	theme	potential	67:75	arg1	grafts					101:106	potential small-diameter vascular grafts	67:106	potential small-diameter vascular grafts	67:106	Hierarchical-structured bacterial cellulose/potato starch tubes as potential small-diameter vascular grafts.
35074114	1	48	theme	<6 mm	153:157	arg1	implants					169:176	long-term patent small-diameter (<6 mm) vascular implants	120:176	long-term patent small-diameter (<6 mm) vascular implants	120:176	To achieve long-term patent small-diameter (<6 mm) vascular implants, biomimetic vascular grafts have gained much attention in promoting in situ blood vessel regeneration.
35074114	4	49	theme	tissue	725:730	arg1	engineering					732:742	vascular tissue engineering	716:742	vascular tissue engineering	716:742	The composites displayed a morphology, water content, thermal properties, mechanical properties, and biocompatibility appropriate for vascular tissue engineering.
35074114	5	50	theme	new	1029:1031	arg1	capillaries					1033:1043	rich new capillaries	1024:1043	rich new capillaries	1024:1043	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	5	51	dep	in	925:926	arg1	vivo					928:931	vivo	928:931	vivo	928:931	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	5	52	theme	complete	961:968	arg1	monolayer					982:990	complete endothelium monolayer	961:990	complete endothelium monolayer	961:990	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	5	53	theme	organized	993:1001	arg1	cells					1017:1021	organized smooth muscle cells	993:1021	organized smooth muscle cells	993:1021	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	5	54	theme	rich	1024:1027	arg1	capillaries					1033:1043	rich new capillaries	1024:1043	rich new capillaries	1024:1043	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	1	55	dep	in	246:247	arg1	situ					249:252	situ	249:252	situ	249:252	To achieve long-term patent small-diameter (<6 mm) vascular implants, biomimetic vascular grafts have gained much attention in promoting in situ blood vessel regeneration.
35074114	1	56	theme	vascular	160:167	arg1	implants					169:176	long-term patent small-diameter (<6 mm) vascular implants	120:176	long-term patent small-diameter (<6 mm) vascular implants	120:176	To achieve long-term patent small-diameter (<6 mm) vascular implants, biomimetic vascular grafts have gained much attention in promoting in situ blood vessel regeneration.
35074114	1	57	theme	blood	254:258	arg1	regeneration					267:278	in situ blood vessel regeneration	246:278	in situ blood vessel regeneration	246:278	To achieve long-term patent small-diameter (<6 mm) vascular implants, biomimetic vascular grafts have gained much attention in promoting in situ blood vessel regeneration.
35074114	5	58	theme	blood	896:900	arg1	regeneration					909:920	rapid blood vessel regeneration	890:920	rapid blood vessel regeneration	890:920	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	5	59	with	grafts	773:778	arg1	layer					848:852	a circumferential macroporous outer layer	812:852	a circumferential macroporous outer layer	812:852	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	5	59	with	grafts	773:778	arg1	surface					800:806	a dense inner surface	786:806	a dense inner surface	786:806	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	5	60	theme	smooth	1003:1008	arg1	cells					1017:1021	organized smooth muscle cells	993:1021	organized smooth muscle cells	993:1021	Most importantly, the BC/PS grafts, with a dense inner surface and a circumferential macroporous outer layer, possessed 75% patency and promoted rapid blood vessel regeneration in in vivo assessment on rabbits, with complete endothelium monolayer, organized smooth muscle cells, rich new capillaries, and deposited extracellular matrix.
35074114	1	61	theme	vessel	260:265	arg1	regeneration					267:278	in situ blood vessel regeneration	246:278	in situ blood vessel regeneration	246:278	To achieve long-term patent small-diameter (<6 mm) vascular implants, biomimetic vascular grafts have gained much attention in promoting in situ blood vessel regeneration.
35074114	0	62	theme	vascular	92:99	arg1	grafts					101:106	potential small-diameter vascular grafts	67:106	potential small-diameter vascular grafts	67:106	Hierarchical-structured bacterial cellulose/potato starch tubes as potential small-diameter vascular grafts.
35074114	6	63	theme	great	1169:1173	arg1	grafts					1221:1226	artificial small-diameter vascular grafts	1186:1226	artificial small-diameter vascular grafts	1186:1226	Collectively, these findings demonstrate that hierarchical-structured BC/PS tubes hold great promise as artificial small-diameter vascular grafts.
35074114	6	63	theme	great	1169:1173	arg1	promise					1175:1181	hold great promise	1164:1181	hold great promise	1164:1181	Collectively, these findings demonstrate that hierarchical-structured BC/PS tubes hold great promise as artificial small-diameter vascular grafts.
35074114	4	64	theme	mechanical	656:665	arg1	properties					667:676	mechanical properties	656:676	mechanical properties	656:676	The composites displayed a morphology, water content, thermal properties, mechanical properties, and biocompatibility appropriate for vascular tissue engineering.
33805524	8	0	theme	sorption	1065:1072	arg1	performance					1074:1084	sorption performance	1065:1084	sorption performance	1065:1084	Uranyl desorption (using 0.2 M HCl solutions) is achieved within 20-30 min; the sorbents can be recycled for at least five cycles (5-6% loss in sorption performance, complete desorption).
33805524	4	1	theme	textural	528:535	arg1	properties					537:546	textural properties	528:546	textural properties	528:546	Functional groups are characterized by FTIR spectroscopy while textural properties are qualified by N2 adsorption.
33805524	2	2	dep	preparing	309:317	arg1	VI					361:362	VI	361:362	VI	361:362	The phosphorylation of guar gum combined with its association to chitosan allows preparing an efficient sorbent for the removal of U(VI) from slightly acidic solutions.
33805524	11	3	theme	valuable	1501:1508	arg1	metals					1510:1515	valuable metals	1501:1515	valuable metals	1501:1515	The magnetic composite is highly promising as antimicrobial support and for recovery of valuable metals.
33805524	8	4	theme	0.2	946:948	arg1	M					950:950	M	950:950	M	950:950	Uranyl desorption (using 0.2 M HCl solutions) is achieved within 20-30 min; the sorbents can be recycled for at least five cycles (5-6% loss in sorption performance, complete desorption).
33805524	11	5	theme	metals	1510:1515	arg1	recovery					1489:1496	recovery	1489:1496	recovery of valuable metals	1489:1515	The magnetic composite is highly promising as antimicrobial support and for recovery of valuable metals.
33805524	8	6	theme	HCl	952:954	arg1	solutions					956:964	0.2 M HCl solutions	946:964	0.2 M HCl solutions	946:964	Uranyl desorption (using 0.2 M HCl solutions) is achieved within 20-30 min; the sorbents can be recycled for at least five cycles (5-6% loss in sorption performance, complete desorption).
33805524	9	7	dep	U	1179:1179	arg1	VI					1181:1182	VI	1181:1182	VI	1181:1182	In multi-component solutions, the sorbents show marked preference for U(VI) and Nd(III) over alkali-earth metals and Si(IV).
33805524	0	8	theme	Antibacterial	92:104	arg1	Applications					106:117	Antibacterial Applications	92:117	Antibacterial Applications	92:117	Phosphorylation of Guar Gum/Magnetite/Chitosan Nanocomposites for Uranium (VI) Sorption and Antibacterial Applications.
33805524	4	9	theme	FTIR	504:507	arg1	spectroscopy					509:520	FTIR spectroscopy	504:520	FTIR spectroscopy	504:520	Functional groups are characterized by FTIR spectroscopy while textural properties are qualified by N2 adsorption.
33805524	10	10	theme	antibacterial	1295:1307	arg1	effects					1309:1315	antibacterial effects	1295:1315	antibacterial effects	1295:1315	The zone of exclusion method shows that magnetic sorbent has antibacterial effects against both Gram+ and Gram- bacteria, contrary to non-magnetic material (only Gram+ bacteria).
33805524	5	11	theme	amine	627:631	arg1	deprotonation					610:622	deprotonation	610:622	deprotonation of amine and phosphonate groups	610:654	The optimum pH is close to 4 (deprotonation of amine and phosphonate groups).
33805524	10	12	contain	has	1291:1293	arg1	sorbent					1283:1289	magnetic sorbent	1274:1289	magnetic sorbent	1274:1289	The zone of exclusion method shows that magnetic sorbent has antibacterial effects against both Gram+ and Gram- bacteria, contrary to non-magnetic material (only Gram+ bacteria).
33805524	10	12	contain	has	1291:1293	arg2	effects					1309:1315	antibacterial effects	1295:1315	antibacterial effects	1295:1315	The zone of exclusion method shows that magnetic sorbent has antibacterial effects against both Gram+ and Gram- bacteria, contrary to non-magnetic material (only Gram+ bacteria).
33805524	10	13	theme	method	1256:1261	arg1	zone					1238:1241	The zone	1234:1241	The zone of exclusion method	1234:1261	The zone of exclusion method shows that magnetic sorbent has antibacterial effects against both Gram+ and Gram- bacteria, contrary to non-magnetic material (only Gram+ bacteria).
33805524	5	14	theme	phosphonate	637:647	arg1	groups					649:654	phosphonate groups	637:654	phosphonate groups	637:654	The optimum pH is close to 4 (deprotonation of amine and phosphonate groups).
33805524	8	15	theme	M	950:950	arg1	solutions					956:964	0.2 M HCl solutions	946:964	0.2 M HCl solutions	946:964	Uranyl desorption (using 0.2 M HCl solutions) is achieved within 20-30 min; the sorbents can be recycled for at least five cycles (5-6% loss in sorption performance, complete desorption).
33805524	2	16	theme	acidic	379:384	arg1	solutions					386:394	slightly acidic solutions	370:394	slightly acidic solutions	370:394	The phosphorylation of guar gum combined with its association to chitosan allows preparing an efficient sorbent for the removal of U(VI) from slightly acidic solutions.
33805524	8	17	theme	complete	1087:1094	arg1	desorption					1096:1105	complete desorption	1087:1105	complete desorption	1087:1105	Uranyl desorption (using 0.2 M HCl solutions) is achieved within 20-30 min; the sorbents can be recycled for at least five cycles (5-6% loss in sorption performance, complete desorption).
33805524	9	18	theme	alkali-earth	1202:1213	arg1	metals					1215:1220	alkali-earth metals	1202:1220	alkali-earth metals	1202:1220	In multi-component solutions, the sorbents show marked preference for U(VI) and Nd(III) over alkali-earth metals and Si(IV).
33805524	5	19	theme	groups	649:654	arg1	deprotonation					610:622	deprotonation	610:622	deprotonation of amine and phosphonate groups	610:654	The optimum pH is close to 4 (deprotonation of amine and phosphonate groups).
33805524	7	20	theme	Langmuir	902:909	arg1	equation					911:918	Langmuir equation	902:918	Langmuir equation	902:918	Maximum sorption capacities are close to 1.28 and 1.16 mmol U g-1 (non-magnetic and magnetic, respectively), while the sorption isotherms are fitted by Langmuir equation.
33805524	1	21	theme	environmental	178:190	arg1	challenges					192:201	the environmental challenges	174:201	the environmental challenges of wastewater treatment	174:225	The development of new materials is needed to address the environmental challenges of wastewater treatment.
33805524	8	22	dep	loss	1057:1060	arg1	desorption					1096:1105	complete desorption	1087:1105	complete desorption	1087:1105	Uranyl desorption (using 0.2 M HCl solutions) is achieved within 20-30 min; the sorbents can be recycled for at least five cycles (5-6% loss in sorption performance, complete desorption).
33805524	6	23	theme	contact	694:700	arg1	min					687:689	60 min	684:689	60 min of contact	684:700	Uptake kinetics are fast (60 min of contact), fitted by a pseudo-first order rate equation.
33805524	6	23	theme	contact	694:700	arg1	kinetics					665:672	Uptake kinetics	658:672	Uptake kinetics	658:672	Uptake kinetics are fast (60 min of contact), fitted by a pseudo-first order rate equation.
33805524	3	24	theme	nanoparticles	428:440	arg1	incorporation					401:413	The incorporation	397:413	The incorporation of magnetite nanoparticles	397:440	The incorporation of magnetite nanoparticles enhances solid/liquid.
33805524	8	25	theme	Uranyl	921:926	arg1	desorption					928:937	Uranyl desorption	921:937	Uranyl desorption (using 0.2 M HCl solutions)	921:965	Uranyl desorption (using 0.2 M HCl solutions) is achieved within 20-30 min; the sorbents can be recycled for at least five cycles (5-6% loss in sorption performance, complete desorption).
33805524	10	26	theme	Gram+	1330:1334	arg1	bacteria					1346:1353	both Gram+ and Gram- bacteria	1325:1353	bacteria	1346:1353	The zone of exclusion method shows that magnetic sorbent has antibacterial effects against both Gram+ and Gram- bacteria, contrary to non-magnetic material (only Gram+ bacteria).
33805524	10	27	theme	magnetic	1274:1281	arg1	sorbent					1283:1289	magnetic sorbent	1274:1289	magnetic sorbent	1274:1289	The zone of exclusion method shows that magnetic sorbent has antibacterial effects against both Gram+ and Gram- bacteria, contrary to non-magnetic material (only Gram+ bacteria).
33805524	0	28	dep	Sorption	79:86	arg1	VI					75:76	VI	75:76	VI	75:76	Phosphorylation of Guar Gum/Magnetite/Chitosan Nanocomposites for Uranium (VI) Sorption and Antibacterial Applications.
33805524	9	29	theme	marked	1157:1162	arg1	preference					1164:1173	marked preference	1157:1173	marked preference for U(VI) and Nd(III) over alkali-earth metals and Si(IV)	1157:1231	In multi-component solutions, the sorbents show marked preference for U(VI) and Nd(III) over alkali-earth metals and Si(IV).
33805524	0	30	theme	Gum/Magnetite/Chitosan	24:45	arg1	Phosphorylation					0:14	Phosphorylation	0:14	Phosphorylation of Guar Gum/Magnetite/Chitosan	0:45	Phosphorylation of Guar Gum/Magnetite/Chitosan Nanocomposites for Uranium (VI) Sorption and Antibacterial Applications.
33805524	7	31	theme	1.16	800:803	arg1	mmol					805:808	mmol	805:808	mmol	805:808	Maximum sorption capacities are close to 1.28 and 1.16 mmol U g-1 (non-magnetic and magnetic, respectively), while the sorption isotherms are fitted by Langmuir equation.
33805524	10	32	theme	non-magnetic	1368:1379	arg1	bacteria					1402:1409	only Gram+ bacteria	1391:1409	only Gram+ bacteria	1391:1409	The zone of exclusion method shows that magnetic sorbent has antibacterial effects against both Gram+ and Gram- bacteria, contrary to non-magnetic material (only Gram+ bacteria).
33805524	10	32	theme	non-magnetic	1368:1379	arg1	material					1381:1388	non-magnetic material	1368:1388	non-magnetic material (only Gram+ bacteria)	1368:1410	The zone of exclusion method shows that magnetic sorbent has antibacterial effects against both Gram+ and Gram- bacteria, contrary to non-magnetic material (only Gram+ bacteria).
33805524	9	33	theme	multi-component	1112:1126	arg1	solutions					1128:1136	multi-component solutions	1112:1136	multi-component solutions	1112:1136	In multi-component solutions, the sorbents show marked preference for U(VI) and Nd(III) over alkali-earth metals and Si(IV).
33805524	2	34	theme	efficient	322:330	arg1	sorbent					332:338	an efficient sorbent	319:338	an efficient sorbent for the removal of U	319:359	The phosphorylation of guar gum combined with its association to chitosan allows preparing an efficient sorbent for the removal of U(VI) from slightly acidic solutions.
33805524	1	35	theme	wastewater	206:215	arg1	treatment					217:225	wastewater treatment	206:225	wastewater treatment	206:225	The development of new materials is needed to address the environmental challenges of wastewater treatment.
33805524	0	36	theme	Guar	19:22	arg1	Gum/Magnetite/Chitosan					24:45	Guar Gum/Magnetite/Chitosan	19:45	Guar Gum/Magnetite/Chitosan	19:45	Phosphorylation of Guar Gum/Magnetite/Chitosan Nanocomposites for Uranium (VI) Sorption and Antibacterial Applications.
33805524	7	37	theme	mmol	805:808	arg1	g-1					812:814	1.16 mmol U g-1	800:814	1.16 mmol U g-1 (non-magnetic and magnetic, respectively)	800:856	Maximum sorption capacities are close to 1.28 and 1.16 mmol U g-1 (non-magnetic and magnetic, respectively), while the sorption isotherms are fitted by Langmuir equation.
33805524	1	38	theme	treatment	217:225	arg1	challenges					192:201	the environmental challenges	174:201	the environmental challenges of wastewater treatment	174:225	The development of new materials is needed to address the environmental challenges of wastewater treatment.
33805524	4	39	theme	Functional	465:474	arg1	groups					476:481	Functional groups	465:481	Functional groups	465:481	Functional groups are characterized by FTIR spectroscopy while textural properties are qualified by N2 adsorption.
33805524	2	40	theme	gum	256:258	arg1	phosphorylation					232:246	The phosphorylation	228:246	The phosphorylation of guar gum combined with its association to chitosan	228:300	The phosphorylation of guar gum combined with its association to chitosan allows preparing an efficient sorbent for the removal of U(VI) from slightly acidic solutions.
33805524	7	41	theme	U	810:810	arg1	g-1					812:814	1.16 mmol U g-1	800:814	1.16 mmol U g-1 (non-magnetic and magnetic, respectively)	800:856	Maximum sorption capacities are close to 1.28 and 1.16 mmol U g-1 (non-magnetic and magnetic, respectively), while the sorption isotherms are fitted by Langmuir equation.
33805524	3	42	theme	magnetite	418:426	arg1	nanoparticles					428:440	magnetite nanoparticles	418:440	magnetite nanoparticles	418:440	The incorporation of magnetite nanoparticles enhances solid/liquid.
33805524	4	43	theme	N2	565:566	arg1	adsorption					568:577	N2 adsorption	565:577	N2 adsorption	565:577	Functional groups are characterized by FTIR spectroscopy while textural properties are qualified by N2 adsorption.
33805524	7	44	theme	sorption	869:876	arg1	isotherms					878:886	the sorption isotherms	865:886	the sorption isotherms	865:886	Maximum sorption capacities are close to 1.28 and 1.16 mmol U g-1 (non-magnetic and magnetic, respectively), while the sorption isotherms are fitted by Langmuir equation.
33805524	8	45	from	loss	1057:1060	arg1	performance					1074:1084	sorption performance	1065:1084	sorption performance	1065:1084	Uranyl desorption (using 0.2 M HCl solutions) is achieved within 20-30 min; the sorbents can be recycled for at least five cycles (5-6% loss in sorption performance, complete desorption).
33805524	2	46	theme	guar	251:254	arg1	gum					256:258	guar gum	251:258	guar gum combined with its association to chitosan	251:300	The phosphorylation of guar gum combined with its association to chitosan allows preparing an efficient sorbent for the removal of U(VI) from slightly acidic solutions.
33805524	11	47	theme	antimicrobial	1459:1471	arg1	support					1473:1479	antimicrobial support	1459:1479	antimicrobial support	1459:1479	The magnetic composite is highly promising as antimicrobial support and for recovery of valuable metals.
33805524	10	48	theme	Gram+	1396:1400	arg1	bacteria					1402:1409	only Gram+ bacteria	1391:1409	only Gram+ bacteria	1391:1409	The zone of exclusion method shows that magnetic sorbent has antibacterial effects against both Gram+ and Gram- bacteria, contrary to non-magnetic material (only Gram+ bacteria).
33805524	10	48	theme	Gram+	1396:1400	arg1	material					1381:1388	non-magnetic material	1368:1388	non-magnetic material (only Gram+ bacteria)	1368:1410	The zone of exclusion method shows that magnetic sorbent has antibacterial effects against both Gram+ and Gram- bacteria, contrary to non-magnetic material (only Gram+ bacteria).
33805524	8	49	dep	desorption	928:937	arg1	using					940:944	using	940:944	using 0.2 M HCl solutions	940:964	Uranyl desorption (using 0.2 M HCl solutions) is achieved within 20-30 min; the sorbents can be recycled for at least five cycles (5-6% loss in sorption performance, complete desorption).
33805524	5	50	theme	optimum	584:590	arg1	4					607:607	4	607:607	4	607:607	The optimum pH is close to 4 (deprotonation of amine and phosphonate groups).
33805524	5	50	theme	optimum	584:590	arg1	pH					592:593	The optimum pH	580:593	The optimum pH	580:593	The optimum pH is close to 4 (deprotonation of amine and phosphonate groups).
33805524	2	51	theme	U	359:359	arg1	removal					348:354	the removal	344:354	the removal of U	344:359	The phosphorylation of guar gum combined with its association to chitosan allows preparing an efficient sorbent for the removal of U(VI) from slightly acidic solutions.
33805524	0	52	theme	Uranium	66:72	arg1	Sorption					79:86	Uranium (VI) Sorption	66:86	Uranium (VI) Sorption	66:86	Phosphorylation of Guar Gum/Magnetite/Chitosan Nanocomposites for Uranium (VI) Sorption and Antibacterial Applications.
33805524	7	53	theme	sorption	758:765	arg1	capacities					767:776	Maximum sorption capacities	750:776	Maximum sorption capacities	750:776	Maximum sorption capacities are close to 1.28 and 1.16 mmol U g-1 (non-magnetic and magnetic, respectively), while the sorption isotherms are fitted by Langmuir equation.
33805524	10	54	theme	only	1391:1394	arg1	bacteria					1402:1409	only Gram+ bacteria	1391:1409	only Gram+ bacteria	1391:1409	The zone of exclusion method shows that magnetic sorbent has antibacterial effects against both Gram+ and Gram- bacteria, contrary to non-magnetic material (only Gram+ bacteria).
33805524	10	54	theme	only	1391:1394	arg1	material					1381:1388	non-magnetic material	1368:1388	non-magnetic material (only Gram+ bacteria)	1368:1410	The zone of exclusion method shows that magnetic sorbent has antibacterial effects against both Gram+ and Gram- bacteria, contrary to non-magnetic material (only Gram+ bacteria).
33805524	10	55	theme	Gram-	1340:1344	arg1	bacteria					1346:1353	both Gram+ and Gram- bacteria	1325:1353	bacteria	1346:1353	The zone of exclusion method shows that magnetic sorbent has antibacterial effects against both Gram+ and Gram- bacteria, contrary to non-magnetic material (only Gram+ bacteria).
33805524	8	56	theme	5-6	1052:1054	arg1	%					1055:1055	%	1055:1055	%	1055:1055	Uranyl desorption (using 0.2 M HCl solutions) is achieved within 20-30 min; the sorbents can be recycled for at least five cycles (5-6% loss in sorption performance, complete desorption).
33805524	6	57	theme	rate	735:738	arg1	equation					740:747	a pseudo-first order rate equation	714:747	a pseudo-first order rate equation	714:747	Uptake kinetics are fast (60 min of contact), fitted by a pseudo-first order rate equation.
33805524	1	58	theme	new	139:141	arg1	materials					143:151	new materials	139:151	new materials	139:151	The development of new materials is needed to address the environmental challenges of wastewater treatment.
33805524	7	59	dep	g-1	812:814	arg1	magnetic					834:841	magnetic	834:841	magnetic	834:841	Maximum sorption capacities are close to 1.28 and 1.16 mmol U g-1 (non-magnetic and magnetic, respectively), while the sorption isotherms are fitted by Langmuir equation.
33805524	7	59	dep	g-1	812:814	arg1	non-magnetic					817:828	non-magnetic	817:828	non-magnetic	817:828	Maximum sorption capacities are close to 1.28 and 1.16 mmol U g-1 (non-magnetic and magnetic, respectively), while the sorption isotherms are fitted by Langmuir equation.
33805524	6	60	theme	order	729:733	arg1	equation					740:747	a pseudo-first order rate equation	714:747	a pseudo-first order rate equation	714:747	Uptake kinetics are fast (60 min of contact), fitted by a pseudo-first order rate equation.
33805524	1	61	theme	materials	143:151	arg1	development					124:134	The development	120:134	The development of new materials	120:151	The development of new materials is needed to address the environmental challenges of wastewater treatment.
33805524	7	62	theme	Maximum	750:756	arg1	capacities					767:776	Maximum sorption capacities	750:776	Maximum sorption capacities	750:776	Maximum sorption capacities are close to 1.28 and 1.16 mmol U g-1 (non-magnetic and magnetic, respectively), while the sorption isotherms are fitted by Langmuir equation.
33805524	11	63	theme	magnetic	1417:1424	arg1	composite					1426:1434	The magnetic composite	1413:1434	The magnetic composite	1413:1434	The magnetic composite is highly promising as antimicrobial support and for recovery of valuable metals.
33805524	11	63	theme	magnetic	1417:1424	arg1	promising					1446:1454	promising	1446:1454	promising	1446:1454	The magnetic composite is highly promising as antimicrobial support and for recovery of valuable metals.
33805524	6	64	theme	pseudo-first	716:727	arg1	equation					740:747	a pseudo-first order rate equation	714:747	a pseudo-first order rate equation	714:747	Uptake kinetics are fast (60 min of contact), fitted by a pseudo-first order rate equation.
33805524	10	65	theme	exclusion	1246:1254	arg1	method					1256:1261	exclusion method	1246:1261	exclusion method	1246:1261	The zone of exclusion method shows that magnetic sorbent has antibacterial effects against both Gram+ and Gram- bacteria, contrary to non-magnetic material (only Gram+ bacteria).
33805524	6	66	theme	Uptake	658:663	arg1	min					687:689	60 min	684:689	60 min of contact	684:700	Uptake kinetics are fast (60 min of contact), fitted by a pseudo-first order rate equation.
33805524	6	66	theme	Uptake	658:663	arg1	kinetics					665:672	Uptake kinetics	658:672	Uptake kinetics	658:672	Uptake kinetics are fast (60 min of contact), fitted by a pseudo-first order rate equation.
33805524	8	67	theme	%	1055:1055	arg1	cycles					1044:1049	at least five cycles	1030:1049	at least five cycles (5-6% loss in sorption performance, complete desorption)	1030:1106	Uranyl desorption (using 0.2 M HCl solutions) is achieved within 20-30 min; the sorbents can be recycled for at least five cycles (5-6% loss in sorption performance, complete desorption).
33805524	8	67	theme	%	1055:1055	arg1	loss					1057:1060	5-6% loss	1052:1060	5-6% loss in sorption performance	1052:1084	Uranyl desorption (using 0.2 M HCl solutions) is achieved within 20-30 min; the sorbents can be recycled for at least five cycles (5-6% loss in sorption performance, complete desorption).
33805524	6	68	dep	fast	678:681	arg1	min					687:689	60 min	684:689	60 min of contact	684:700	Uptake kinetics are fast (60 min of contact), fitted by a pseudo-first order rate equation.
33805524	6	68	dep	fast	678:681	arg1	kinetics					665:672	Uptake kinetics	658:672	Uptake kinetics	658:672	Uptake kinetics are fast (60 min of contact), fitted by a pseudo-first order rate equation.
33805524	6	68	dep	fast	678:681	arg1	fitted					704:709	fitted	704:709	fitted by a pseudo-first order rate equation	704:747	Uptake kinetics are fast (60 min of contact), fitted by a pseudo-first order rate equation.
33805524	5	69	dep	4	607:607	arg1	deprotonation					610:622	deprotonation	610:622	deprotonation of amine and phosphonate groups	610:654	The optimum pH is close to 4 (deprotonation of amine and phosphonate groups).
32199919	2	0	from	galactose	409:417	arg1	ratio					430:434	a molar ratio	422:434	a molar ratio of 1.2: 4.4: 0.9: 0.9: 2.6	422:461	Our results indicated that ASALP was a homogeneous polysaccharide with a molecular weight of 1.2 × 105 Da composed of rhamnose, arabinose, xylose, glucose and galactose in a molar ratio of 1.2: 4.4: 0.9: 0.9: 2.6.
32199919	2	1	from	glucose	397:403	arg1	ratio					430:434	a molar ratio	422:434	a molar ratio of 1.2: 4.4: 0.9: 0.9: 2.6	422:461	Our results indicated that ASALP was a homogeneous polysaccharide with a molecular weight of 1.2 × 105 Da composed of rhamnose, arabinose, xylose, glucose and galactose in a molar ratio of 1.2: 4.4: 0.9: 0.9: 2.6.
32199919	2	2	theme	1.2 × 105 Da	343:354	arg1	weight					333:338	a molecular weight	321:338	a molecular weight of 1.2 × 105 Da composed of rhamnose, arabinose, xylose, glucose and galactose in a molar ratio of 1.2: 4.4: 0.9: 0.9: 2.6	321:461	Our results indicated that ASALP was a homogeneous polysaccharide with a molecular weight of 1.2 × 105 Da composed of rhamnose, arabinose, xylose, glucose and galactose in a molar ratio of 1.2: 4.4: 0.9: 0.9: 2.6.
32199919	5	3	theme	cytokines	1099:1107	arg1	production					1063:1072	the production	1059:1072	the production of the anti-inflammatory cytokines IL-10	1059:1113	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	7	4	theme	treatment	1414:1422	arg1	group					1424:1428	LPS + ASALP treatment group	1402:1428	LPS + ASALP treatment group	1402:1428	Lower levels of Proteobacteria, Staphylococcus and Bacteroidetes were detected in LPS + ASALP treatment group.
32199919	0	5	from	activity	18:25	arg1	microbiota					105:114	gut microbiota	101:114	gut microbiota of mice with inflammation	101:140	Anti-inflammatory activity of alkali-soluble polysaccharides from Arctium lappa L. and its effect on gut microbiota of mice with inflammation.
32199919	0	5	from	activity	18:25	arg1	Arctium					66:72	Arctium	66:72	Arctium	66:72	Anti-inflammatory activity of alkali-soluble polysaccharides from Arctium lappa L. and its effect on gut microbiota of mice with inflammation.
32199919	4	6	dep	In	653:654	arg1	vitro					656:660	vitro	656:660	vitro	656:660	In vitro and in vivo assay showed that ASALP could effectively alleviate inflammation by improving the dysregulation of pro-inflammatory and anti-inflammatory cytokines.
32199919	0	7	theme	gut	101:103	arg1	microbiota					105:114	gut microbiota	101:114	gut microbiota of mice with inflammation	101:140	Anti-inflammatory activity of alkali-soluble polysaccharides from Arctium lappa L. and its effect on gut microbiota of mice with inflammation.
32199919	5	8	theme	mice	1039:1042	arg1	serum					1017:1021	the serum	1013:1021	the serum of inflammatory mice	1013:1042	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	5	9	theme	oxide	892:896	arg1	production					871:880	the production	867:880	the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice	867:1042	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	5	10	theme	pro-inflammatory	907:922	arg1	cytokines					924:932	pro-inflammatory cytokines	907:932	pro-inflammatory cytokines (IL-6, IL-1β and TNF-α)	907:956	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	5	10	theme	pro-inflammatory	907:922	arg1	TNF-α					951:955	TNF-α	951:955	TNF-α	951:955	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	5	10	theme	pro-inflammatory	907:922	arg1	IL-1β					941:945	IL-1β	941:945	IL-1β	941:945	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	5	10	theme	pro-inflammatory	907:922	arg1	IL-6					935:938	IL-6	935:938	IL-6	935:938	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	2	11	with	polysaccharide	301:314	arg1	weight					333:338	a molecular weight	321:338	a molecular weight of 1.2 × 105 Da composed of rhamnose, arabinose, xylose, glucose and galactose in a molar ratio of 1.2: 4.4: 0.9: 0.9: 2.6	321:461	Our results indicated that ASALP was a homogeneous polysaccharide with a molecular weight of 1.2 × 105 Da composed of rhamnose, arabinose, xylose, glucose and galactose in a molar ratio of 1.2: 4.4: 0.9: 0.9: 2.6.
32199919	2	12	theme	2.6	459:461	arg1	ratio					430:434	a molar ratio	422:434	a molar ratio of 1.2: 4.4: 0.9: 0.9: 2.6	422:461	Our results indicated that ASALP was a homogeneous polysaccharide with a molecular weight of 1.2 × 105 Da composed of rhamnose, arabinose, xylose, glucose and galactose in a molar ratio of 1.2: 4.4: 0.9: 0.9: 2.6.
32199919	7	13	theme	Bacteroidetes	1371:1383	arg1	levels					1326:1331	Lower levels	1320:1331	Lower levels of Proteobacteria, Staphylococcus and Bacteroidetes	1320:1383	Lower levels of Proteobacteria, Staphylococcus and Bacteroidetes were detected in LPS + ASALP treatment group.
32199919	4	14	theme	pro-inflammatory	773:788	arg1	cytokines					812:820	pro-inflammatory and anti-inflammatory cytokines	773:820	pro-inflammatory and anti-inflammatory cytokines	773:820	In vitro and in vivo assay showed that ASALP could effectively alleviate inflammation by improving the dysregulation of pro-inflammatory and anti-inflammatory cytokines.
32199919	9	15	theme	ASALP	1645:1649	arg1	application					1630:1640	application	1630:1640	application of ASALP	1630:1649	Our study could provide the basis for the subsequent research and application of ASALP.
32199919	9	15	theme	ASALP	1645:1649	arg1	research					1617:1624	the subsequent research	1602:1624	the subsequent research	1602:1624	Our study could provide the basis for the subsequent research and application of ASALP.
32199919	4	16	theme	In	653:654	arg1	assay					674:678	In vitro and in vivo assay	653:678	In vitro and in vivo assay	653:678	In vitro and in vivo assay showed that ASALP could effectively alleviate inflammation by improving the dysregulation of pro-inflammatory and anti-inflammatory cytokines.
32199919	6	17	theme	Lactobacillus	1247:1259	arg1	abundance					1195:1203	the relative abundance	1182:1203	the relative abundance of Firmicutes, Alistipes, Odoribacter and Lactobacillus in mice	1182:1267	The results from 16S rRNA (V3-V4) amplicon sequencing showed that the relative abundance of Firmicutes, Alistipes, Odoribacter and Lactobacillus in mice was significantly increased after ASALP treatment.
32199919	0	18	from	Arctium	66:72	arg1	effect					91:96	its effect	87:96	its effect on gut microbiota of mice with inflammation	87:140	Anti-inflammatory activity of alkali-soluble polysaccharides from Arctium lappa L. and its effect on gut microbiota of mice with inflammation.
32199919	0	18	from	Arctium	66:72	arg1	activity					18:25	Anti-inflammatory activity	0:25	Anti-inflammatory activity of alkali-soluble polysaccharides from Arctium lappa L.	0:81	Anti-inflammatory activity of alkali-soluble polysaccharides from Arctium lappa L. and its effect on gut microbiota of mice with inflammation.
32199919	0	18	from	Arctium	66:72	arg1	polysaccharides					45:59	alkali-soluble polysaccharides	30:59	alkali-soluble polysaccharides from Arctium lappa L.	30:81	Anti-inflammatory activity of alkali-soluble polysaccharides from Arctium lappa L. and its effect on gut microbiota of mice with inflammation.
32199919	6	19	theme	amplicon	1150:1157	arg1	sequencing					1159:1168	16S rRNA (V3-V4) amplicon sequencing	1133:1168	16S rRNA (V3-V4) amplicon sequencing	1133:1168	The results from 16S rRNA (V3-V4) amplicon sequencing showed that the relative abundance of Firmicutes, Alistipes, Odoribacter and Lactobacillus in mice was significantly increased after ASALP treatment.
32199919	4	20	theme	in	666:667	arg1	assay					674:678	In vitro and in vivo assay	653:678	In vitro and in vivo assay	653:678	In vitro and in vivo assay showed that ASALP could effectively alleviate inflammation by improving the dysregulation of pro-inflammatory and anti-inflammatory cytokines.
32199919	3	21	dep	consisted	527:535	arg1	α-Araf-					570:576	α-Araf-	570:576	α-Araf-	570:576	The structure characterization indicated that ASALP was mainly consisted of →5-α-L-Araf-(1 → backbone and α-Araf-(1→,→2)-α-Rhap-(1 → T-Glcp-(1→, →3)-β-D-Xylp-(1 → 4)-α-GalpA-(1 → branches.
32199919	3	21	dep	consisted	527:535	arg1	1 → backbone					553:564	1 → backbone	553:564	1 → backbone	553:564	The structure characterization indicated that ASALP was mainly consisted of →5-α-L-Araf-(1 → backbone and α-Araf-(1→,→2)-α-Rhap-(1 → T-Glcp-(1→, →3)-β-D-Xylp-(1 → 4)-α-GalpA-(1 → branches.
32199919	6	22	from	mice	1264:1267	arg1	abundance					1195:1203	the relative abundance	1182:1203	the relative abundance of Firmicutes, Alistipes, Odoribacter and Lactobacillus in mice	1182:1267	The results from 16S rRNA (V3-V4) amplicon sequencing showed that the relative abundance of Firmicutes, Alistipes, Odoribacter and Lactobacillus in mice was significantly increased after ASALP treatment.
32199919	9	23	theme	subsequent	1606:1615	arg1	research					1617:1624	the subsequent research	1602:1624	the subsequent research	1602:1624	Our study could provide the basis for the subsequent research and application of ASALP.
32199919	3	24	theme	structure	468:476	arg1	characterization					478:493	The structure characterization	464:493	The structure characterization	464:493	The structure characterization indicated that ASALP was mainly consisted of →5-α-L-Araf-(1 → backbone and α-Araf-(1→,→2)-α-Rhap-(1 → T-Glcp-(1→, →3)-β-D-Xylp-(1 → 4)-α-GalpA-(1 → branches.
32199919	1	25	theme	alkali-soluble	161:174	arg1	polysaccharide					176:189	an alkali-soluble polysaccharide	158:189	an alkali-soluble polysaccharide (ASALP) from Arctium lappa L.	158:219	In this study, an alkali-soluble polysaccharide (ASALP) from Arctium lappa L. were extracted and purified.
32199919	1	25	theme	alkali-soluble	161:174	arg1	ASALP					192:196	ASALP	192:196	ASALP	192:196	In this study, an alkali-soluble polysaccharide (ASALP) from Arctium lappa L. were extracted and purified.
32199919	2	26	theme	molar	424:428	arg1	ratio					430:434	a molar ratio	422:434	a molar ratio of 1.2: 4.4: 0.9: 0.9: 2.6	422:461	Our results indicated that ASALP was a homogeneous polysaccharide with a molecular weight of 1.2 × 105 Da composed of rhamnose, arabinose, xylose, glucose and galactose in a molar ratio of 1.2: 4.4: 0.9: 0.9: 2.6.
32199919	8	27	theme	microbial	1491:1499	arg1	diversity					1501:1509	microbial diversity	1491:1509	microbial diversity	1491:1509	ASALP alleviated inflammation by improving the reduction of microbial diversity and affecting the composition of the gut microbiota.
32199919	6	28	theme	V3-V4	1143:1147	arg1	sequencing					1159:1168	16S rRNA (V3-V4) amplicon sequencing	1133:1168	16S rRNA (V3-V4) amplicon sequencing	1133:1168	The results from 16S rRNA (V3-V4) amplicon sequencing showed that the relative abundance of Firmicutes, Alistipes, Odoribacter and Lactobacillus in mice was significantly increased after ASALP treatment.
32199919	0	29	theme	Anti-inflammatory	0:16	arg1	activity					18:25	Anti-inflammatory activity	0:25	Anti-inflammatory activity of alkali-soluble polysaccharides from Arctium lappa L.	0:81	Anti-inflammatory activity of alkali-soluble polysaccharides from Arctium lappa L. and its effect on gut microbiota of mice with inflammation.
32199919	7	30	theme	Proteobacteria	1336:1349	arg1	levels					1326:1331	Lower levels	1320:1331	Lower levels of Proteobacteria, Staphylococcus and Bacteroidetes	1320:1383	Lower levels of Proteobacteria, Staphylococcus and Bacteroidetes were detected in LPS + ASALP treatment group.
32199919	2	31	theme	homogeneous	289:299	arg1	ASALP					277:281	ASALP	277:281	ASALP	277:281	Our results indicated that ASALP was a homogeneous polysaccharide with a molecular weight of 1.2 × 105 Da composed of rhamnose, arabinose, xylose, glucose and galactose in a molar ratio of 1.2: 4.4: 0.9: 0.9: 2.6.
32199919	2	31	theme	homogeneous	289:299	arg1	polysaccharide					301:314	a homogeneous polysaccharide	287:314	a homogeneous polysaccharide with a molecular weight of 1.2 × 105 Da composed of rhamnose, arabinose, xylose, glucose and galactose in a molar ratio of 1.2: 4.4: 0.9: 0.9: 2.6	287:461	Our results indicated that ASALP was a homogeneous polysaccharide with a molecular weight of 1.2 × 105 Da composed of rhamnose, arabinose, xylose, glucose and galactose in a molar ratio of 1.2: 4.4: 0.9: 0.9: 2.6.
32199919	7	32	theme	Staphylococcus	1352:1365	arg1	levels					1326:1331	Lower levels	1320:1331	Lower levels of Proteobacteria, Staphylococcus and Bacteroidetes	1320:1383	Lower levels of Proteobacteria, Staphylococcus and Bacteroidetes were detected in LPS + ASALP treatment group.
32199919	0	33	theme	alkali-soluble	30:43	arg1	polysaccharides					45:59	alkali-soluble polysaccharides	30:59	alkali-soluble polysaccharides from Arctium lappa L.	30:81	Anti-inflammatory activity of alkali-soluble polysaccharides from Arctium lappa L. and its effect on gut microbiota of mice with inflammation.
32199919	6	34	theme	Odoribacter	1231:1241	arg1	abundance					1195:1203	the relative abundance	1182:1203	the relative abundance of Firmicutes, Alistipes, Odoribacter and Lactobacillus in mice	1182:1267	The results from 16S rRNA (V3-V4) amplicon sequencing showed that the relative abundance of Firmicutes, Alistipes, Odoribacter and Lactobacillus in mice was significantly increased after ASALP treatment.
32199919	3	35	dep	1 → backbone	553:564	arg1	→2					581:582	→2	581:582	→2	581:582	The structure characterization indicated that ASALP was mainly consisted of →5-α-L-Araf-(1 → backbone and α-Araf-(1→,→2)-α-Rhap-(1 → T-Glcp-(1→, →3)-β-D-Xylp-(1 → 4)-α-GalpA-(1 → branches.
32199919	3	35	dep	1 → backbone	553:564	arg1	1 → branches					639:650	1 → branches	639:650	1 → branches	639:650	The structure characterization indicated that ASALP was mainly consisted of →5-α-L-Araf-(1 → backbone and α-Araf-(1→,→2)-α-Rhap-(1 → T-Glcp-(1→, →3)-β-D-Xylp-(1 → 4)-α-GalpA-(1 → branches.
32199919	7	36	theme	Lower	1320:1324	arg1	levels					1326:1331	Lower levels	1320:1331	Lower levels of Proteobacteria, Staphylococcus and Bacteroidetes	1320:1383	Lower levels of Proteobacteria, Staphylococcus and Bacteroidetes were detected in LPS + ASALP treatment group.
32199919	8	37	theme	diversity	1501:1509	arg1	reduction					1478:1486	the reduction	1474:1486	the reduction of microbial diversity	1474:1509	ASALP alleviated inflammation by improving the reduction of microbial diversity and affecting the composition of the gut microbiota.
32199919	0	38	theme	mice	119:122	arg1	microbiota					105:114	gut microbiota	101:114	gut microbiota of mice with inflammation	101:140	Anti-inflammatory activity of alkali-soluble polysaccharides from Arctium lappa L. and its effect on gut microbiota of mice with inflammation.
32199919	0	39	from	effect	91:96	arg1	microbiota					105:114	gut microbiota	101:114	gut microbiota of mice with inflammation	101:140	Anti-inflammatory activity of alkali-soluble polysaccharides from Arctium lappa L. and its effect on gut microbiota of mice with inflammation.
32199919	0	39	from	effect	91:96	arg1	Arctium					66:72	Arctium	66:72	Arctium	66:72	Anti-inflammatory activity of alkali-soluble polysaccharides from Arctium lappa L. and its effect on gut microbiota of mice with inflammation.
32199919	5	40	theme	nitric	885:890	arg1	NO					899:900	NO	899:900	NO	899:900	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	5	40	theme	nitric	885:890	arg1	oxide					892:896	nitric oxide	885:896	nitric oxide (NO)	885:901	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	5	41	theme	anti-inflammatory	1081:1097	arg1	cytokines					1099:1107	the anti-inflammatory cytokines	1077:1107	the anti-inflammatory cytokines IL-10	1077:1113	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	6	42	theme	Alistipes	1220:1228	arg1	abundance					1195:1203	the relative abundance	1182:1203	the relative abundance of Firmicutes, Alistipes, Odoribacter and Lactobacillus in mice	1182:1267	The results from 16S rRNA (V3-V4) amplicon sequencing showed that the relative abundance of Firmicutes, Alistipes, Odoribacter and Lactobacillus in mice was significantly increased after ASALP treatment.
32199919	6	43	theme	rRNA	1137:1140	arg1	sequencing					1159:1168	16S rRNA (V3-V4) amplicon sequencing	1133:1168	16S rRNA (V3-V4) amplicon sequencing	1133:1168	The results from 16S rRNA (V3-V4) amplicon sequencing showed that the relative abundance of Firmicutes, Alistipes, Odoribacter and Lactobacillus in mice was significantly increased after ASALP treatment.
32199919	5	44	from	production	871:880	arg1	macrophages					994:1004	lipopolysaccharide (LPS)-treated macrophages	961:1004	lipopolysaccharide (LPS)-treated macrophages	961:1004	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	5	44	from	production	871:880	arg1	serum					1017:1021	the serum	1013:1021	the serum of inflammatory mice	1013:1042	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	0	45	theme	polysaccharides	45:59	arg1	effect					91:96	its effect	87:96	its effect on gut microbiota of mice with inflammation	87:140	Anti-inflammatory activity of alkali-soluble polysaccharides from Arctium lappa L. and its effect on gut microbiota of mice with inflammation.
32199919	0	45	theme	polysaccharides	45:59	arg1	activity					18:25	Anti-inflammatory activity	0:25	Anti-inflammatory activity of alkali-soluble polysaccharides from Arctium lappa L.	0:81	Anti-inflammatory activity of alkali-soluble polysaccharides from Arctium lappa L. and its effect on gut microbiota of mice with inflammation.
32199919	6	46	from	abundance	1195:1203	arg1	mice					1264:1267	mice	1264:1267	mice	1264:1267	The results from 16S rRNA (V3-V4) amplicon sequencing showed that the relative abundance of Firmicutes, Alistipes, Odoribacter and Lactobacillus in mice was significantly increased after ASALP treatment.
32199919	0	47	dep	Arctium	66:72	arg1	L.					80:81	Arctium lappa L.	66:81	Arctium lappa L.	66:81	Anti-inflammatory activity of alkali-soluble polysaccharides from Arctium lappa L. and its effect on gut microbiota of mice with inflammation.
32199919	4	48	theme	cytokines	812:820	arg1	dysregulation					756:768	the dysregulation	752:768	the dysregulation of pro-inflammatory and anti-inflammatory cytokines	752:820	In vitro and in vivo assay showed that ASALP could effectively alleviate inflammation by improving the dysregulation of pro-inflammatory and anti-inflammatory cytokines.
32199919	5	49	theme	-treated	985:992	arg1	macrophages					994:1004	lipopolysaccharide (LPS)-treated macrophages	961:1004	lipopolysaccharide (LPS)-treated macrophages	961:1004	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	1	50	from	Arctium	204:210	arg1	polysaccharide					176:189	an alkali-soluble polysaccharide	158:189	an alkali-soluble polysaccharide (ASALP) from Arctium lappa L.	158:219	In this study, an alkali-soluble polysaccharide (ASALP) from Arctium lappa L. were extracted and purified.
32199919	1	50	from	Arctium	204:210	arg1	ASALP					192:196	ASALP	192:196	ASALP	192:196	In this study, an alkali-soluble polysaccharide (ASALP) from Arctium lappa L. were extracted and purified.
32199919	5	51	theme	inflammatory	1026:1037	arg1	mice					1039:1042	inflammatory mice	1026:1042	inflammatory mice	1026:1042	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	6	52	theme	ASALP	1303:1307	arg1	treatment					1309:1317	ASALP treatment	1303:1317	ASALP treatment	1303:1317	The results from 16S rRNA (V3-V4) amplicon sequencing showed that the relative abundance of Firmicutes, Alistipes, Odoribacter and Lactobacillus in mice was significantly increased after ASALP treatment.
32199919	4	53	theme	anti-inflammatory	794:810	arg1	cytokines					812:820	pro-inflammatory and anti-inflammatory cytokines	773:820	pro-inflammatory and anti-inflammatory cytokines	773:820	In vitro and in vivo assay showed that ASALP could effectively alleviate inflammation by improving the dysregulation of pro-inflammatory and anti-inflammatory cytokines.
32199919	2	54	theme	molecular	323:331	arg1	weight					333:338	a molecular weight	321:338	a molecular weight of 1.2 × 105 Da composed of rhamnose, arabinose, xylose, glucose and galactose in a molar ratio of 1.2: 4.4: 0.9: 0.9: 2.6	321:461	Our results indicated that ASALP was a homogeneous polysaccharide with a molecular weight of 1.2 × 105 Da composed of rhamnose, arabinose, xylose, glucose and galactose in a molar ratio of 1.2: 4.4: 0.9: 0.9: 2.6.
32199919	6	55	theme	16S	1133:1135	arg1	sequencing					1159:1168	16S rRNA (V3-V4) amplicon sequencing	1133:1168	16S rRNA (V3-V4) amplicon sequencing	1133:1168	The results from 16S rRNA (V3-V4) amplicon sequencing showed that the relative abundance of Firmicutes, Alistipes, Odoribacter and Lactobacillus in mice was significantly increased after ASALP treatment.
32199919	6	56	theme	relative	1186:1193	arg1	abundance					1195:1203	the relative abundance	1182:1203	the relative abundance of Firmicutes, Alistipes, Odoribacter and Lactobacillus in mice	1182:1267	The results from 16S rRNA (V3-V4) amplicon sequencing showed that the relative abundance of Firmicutes, Alistipes, Odoribacter and Lactobacillus in mice was significantly increased after ASALP treatment.
32199919	5	57	dep	cytokines	1099:1107	arg1	IL-10					1109:1113	IL-10	1109:1113	the anti-inflammatory cytokines IL-10	1077:1113	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	1	58	dep	Arctium	204:210	arg1	L.					218:219	Arctium lappa L.	204:219	Arctium lappa L.	204:219	In this study, an alkali-soluble polysaccharide (ASALP) from Arctium lappa L. were extracted and purified.
32199919	6	59	from	sequencing	1159:1168	arg1	results					1120:1126	The results	1116:1126	The results from 16S rRNA (V3-V4) amplicon sequencing	1116:1168	The results from 16S rRNA (V3-V4) amplicon sequencing showed that the relative abundance of Firmicutes, Alistipes, Odoribacter and Lactobacillus in mice was significantly increased after ASALP treatment.
32199919	5	60	theme	lipopolysaccharide	961:978	arg1	macrophages					994:1004	lipopolysaccharide (LPS)-treated macrophages	961:1004	lipopolysaccharide (LPS)-treated macrophages	961:1004	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	5	61	theme	cytokines	924:932	arg1	production					871:880	the production	867:880	the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice	867:1042	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	5	62	dep	cytokines	924:932	arg1	cytokines					924:932	pro-inflammatory cytokines	907:932	pro-inflammatory cytokines (IL-6, IL-1β and TNF-α)	907:956	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	5	62	dep	cytokines	924:932	arg1	TNF-α					951:955	TNF-α	951:955	TNF-α	951:955	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	5	62	dep	cytokines	924:932	arg1	IL-1β					941:945	IL-1β	941:945	IL-1β	941:945	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	5	62	dep	cytokines	924:932	arg1	IL-6					935:938	IL-6	935:938	IL-6	935:938	Specifically, ASALP significantly inhibited the production of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in lipopolysaccharide (LPS)-treated macrophages and in the serum of inflammatory mice, but increased the production of the anti-inflammatory cytokines IL-10.
32199919	4	63	dep	in	666:667	arg1	vivo					669:672	vivo	669:672	vivo	669:672	In vitro and in vivo assay showed that ASALP could effectively alleviate inflammation by improving the dysregulation of pro-inflammatory and anti-inflammatory cytokines.
32199919	0	64	with	mice	119:122	arg1	inflammation					129:140	inflammation	129:140	inflammation	129:140	Anti-inflammatory activity of alkali-soluble polysaccharides from Arctium lappa L. and its effect on gut microbiota of mice with inflammation.
32199919	8	65	theme	microbiota	1552:1561	arg1	composition					1529:1539	the composition	1525:1539	the composition of the gut microbiota	1525:1561	ASALP alleviated inflammation by improving the reduction of microbial diversity and affecting the composition of the gut microbiota.
32199919	7	66	theme	LPS + ASALP	1402:1412	arg1	group					1424:1428	LPS + ASALP treatment group	1402:1428	LPS + ASALP treatment group	1402:1428	Lower levels of Proteobacteria, Staphylococcus and Bacteroidetes were detected in LPS + ASALP treatment group.
32199919	8	67	theme	gut	1548:1550	arg1	microbiota					1552:1561	the gut microbiota	1544:1561	the gut microbiota	1544:1561	ASALP alleviated inflammation by improving the reduction of microbial diversity and affecting the composition of the gut microbiota.
32199919	7	68	located	detected	1390:1397	arg1	group					1424:1428	LPS + ASALP treatment group	1402:1428	LPS + ASALP treatment group	1402:1428	Lower levels of Proteobacteria, Staphylococcus and Bacteroidetes were detected in LPS + ASALP treatment group.
32199919	7	68	located	detected	1390:1397	arg2	levels					1326:1331	Lower levels	1320:1331	Lower levels of Proteobacteria, Staphylococcus and Bacteroidetes	1320:1383	Lower levels of Proteobacteria, Staphylococcus and Bacteroidetes were detected in LPS + ASALP treatment group.
32178677	6	0	theme	%	1133:1133	arg1	w/v					1134:1136	1.8%w/v	1130:1136	1.8%w/v	1130:1136	Enzymatic hydrolysate of OMSW fibre was high in D-glucose (5.5% w/v) and D-xylose (1.8%w/v) but deficient in nitrogen and phosphate.
32178677	6	0	theme	%	1133:1133	arg1	D-xylose					1120:1127	D-xylose	1120:1127	D-xylose (1.8%w/v)	1120:1137	Enzymatic hydrolysate of OMSW fibre was high in D-glucose (5.5% w/v) and D-xylose (1.8%w/v) but deficient in nitrogen and phosphate.
32178677	9	1	theme	91 kg	2194:2198	arg1	139 kg					2172:2177	139 kg	2172:2177	139 kg	2172:2177	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	9	1	theme	91 kg	2194:2198	arg1	136 kg					2161:2166	136 kg	2161:2166	136 kg	2161:2166	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	8	2	theme	Zymomonas	1776:1784	arg1	mobilis					1786:1792	Zymomonas mobilis	1776:1792	Zymomonas mobilis	1776:1792	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
32178677	10	3	theme	management	2391:2400	arg1	practices					2402:2410	current waste management practices	2377:2410	current waste management practices	2377:2410	CONCLUSIONS Developing an integrated biorefinery around MSW has the potential to significantly alleviate the environmental burden of current waste management practices.
32178677	4	4	theme	complex	900:906	arg1	feedstock					908:916	fermenting this complex feedstock	884:916	fermenting this complex feedstock	884:916	The resulting OMSW fibre was enzymatically hydrolysed and used to screen diverse microorganisms of biotechnological interest to identify robust species capable of fermenting this complex feedstock.
32178677	10	5	theme	current	2377:2383	arg1	practices					2402:2410	current waste management practices	2377:2410	current waste management practices	2377:2410	CONCLUSIONS Developing an integrated biorefinery around MSW has the potential to significantly alleviate the environmental burden of current waste management practices.
32178677	0	6	theme	solid	73:77	arg1	waste					79:83	municipal solid waste	63:83	municipal solid waste	63:83	Robust microorganisms for biofuel and chemical production from municipal solid waste.
32178677	12	7	theme	biorefining	2638:2648	arg1	platform					2650:2657	an MSW biorefining platform	2631:2657	an MSW biorefining platform	2631:2657	These species are promising candidates for developing an MSW biorefining platform and provide a foundation for future studies aiming to valorise this underexplored feedstock.
32178677	2	8	dep	material	400:407	arg1	%					378:378	%	378:378	%	378:378	The organic fraction of MSW (OMSW) typically comprises ~ 50% lignocellulose-rich material but is underexplored as a biomanufacturing feedstock due to its highly inconsistent and heterogeneous composition.
32178677	5	9	theme	pre-treated	941:951	arg1	OMSW					953:956	The autoclave pre-treated OMSW	927:956	The autoclave pre-treated OMSW	927:956	RESULTS The autoclave pre-treated OMSW fibre contained a polysaccharide fraction comprising 38% cellulose and 4% hemicellulose.
32178677	12	10	theme	underexplored	2727:2739	arg1	feedstock					2741:2749	this underexplored feedstock	2722:2749	this underexplored feedstock	2722:2749	These species are promising candidates for developing an MSW biorefining platform and provide a foundation for future studies aiming to valorise this underexplored feedstock.
32178677	12	11	theme	promising	2595:2603	arg1	species					2583:2589	These species	2577:2589	These species	2577:2589	These species are promising candidates for developing an MSW biorefining platform and provide a foundation for future studies aiming to valorise this underexplored feedstock.
32178677	12	11	theme	promising	2595:2603	arg1	candidates					2605:2614	promising candidates	2595:2614	promising candidates for developing an MSW biorefining platform	2595:2657	These species are promising candidates for developing an MSW biorefining platform and provide a foundation for future studies aiming to valorise this underexplored feedstock.
32178677	9	12	theme	OMSW	2238:2241	arg1	tonne					2229:2233	tonne	2229:2233	tonne of OMSW	2229:2241	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	0	13	from	waste	79:83	arg1	production					47:56	biofuel and chemical production	26:56	biofuel and chemical production from municipal solid waste	26:83	Robust microorganisms for biofuel and chemical production from municipal solid waste.
32178677	7	14	theme	%	1396:1396	arg1	metals					1385:1390	potentially toxic metals	1367:1390	potentially toxic metals (0.6% w/v)	1367:1401	Although relatively low levels of levulinic acid (30 mM) and vanillin (2 mM) were detected and furfural and 5-hydroxymethylfurfural were absent, the hydrolysate contained an abundance of potentially toxic metals (0.6% w/v).
32178677	7	14	theme	%	1396:1396	arg1	w/v					1398:1400	0.6% w/v	1393:1400	0.6% w/v	1393:1400	Although relatively low levels of levulinic acid (30 mM) and vanillin (2 mM) were detected and furfural and 5-hydroxymethylfurfural were absent, the hydrolysate contained an abundance of potentially toxic metals (0.6% w/v).
32178677	8	15	used	used	1488:1491	arg2	Hydrolysate					1404:1414	Hydrolysate	1404:1414	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation	1404:1482	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
32178677	7	16	contain	contained	1341:1349	arg2	abundance					1354:1362	an abundance	1351:1362	an abundance of potentially toxic metals (0.6% w/v)	1351:1401	Although relatively low levels of levulinic acid (30 mM) and vanillin (2 mM) were detected and furfural and 5-hydroxymethylfurfural were absent, the hydrolysate contained an abundance of potentially toxic metals (0.6% w/v).
32178677	7	16	contain	contained	1341:1349	arg1	hydrolysate					1329:1339	the hydrolysate	1325:1339	the hydrolysate	1325:1339	Although relatively low levels of levulinic acid (30 mM) and vanillin (2 mM) were detected and furfural and 5-hydroxymethylfurfural were absent, the hydrolysate contained an abundance of potentially toxic metals (0.6% w/v).
32178677	9	17	theme	growth	1810:1815	arg1	species					1801:1807	Each species	1796:1807	Each species' growth and productivity were characterised	1796:1851	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	7	18	theme	toxic	1379:1383	arg1	metals					1385:1390	potentially toxic metals	1367:1390	potentially toxic metals (0.6% w/v)	1367:1401	Although relatively low levels of levulinic acid (30 mM) and vanillin (2 mM) were detected and furfural and 5-hydroxymethylfurfural were absent, the hydrolysate contained an abundance of potentially toxic metals (0.6% w/v).
32178677	7	18	theme	toxic	1379:1383	arg1	w/v					1398:1400	0.6% w/v	1393:1400	0.6% w/v	1393:1400	Although relatively low levels of levulinic acid (30 mM) and vanillin (2 mM) were detected and furfural and 5-hydroxymethylfurfural were absent, the hydrolysate contained an abundance of potentially toxic metals (0.6% w/v).
32178677	5	19	dep	%	1030:1030	arg1	hemicellulose					1032:1044	hemicellulose	1032:1044	4% hemicellulose	1029:1044	RESULTS The autoclave pre-treated OMSW fibre contained a polysaccharide fraction comprising 38% cellulose and 4% hemicellulose.
32178677	1	20	theme	solid	139:143	arg1	MSW					152:154	MSW	152:154	MSW	152:154	BACKGROUND Worldwide 3.4 billion tonnes of municipal solid waste (MSW) will be produced annually by 2050, however, current approaches to MSW management predominantly involve unsustainable practices like landfilling and incineration.
32178677	1	20	theme	solid	139:143	arg1	waste					145:149	municipal solid waste	129:149	municipal solid waste (MSW)	129:155	BACKGROUND Worldwide 3.4 billion tonnes of municipal solid waste (MSW) will be produced annually by 2050, however, current approaches to MSW management predominantly involve unsustainable practices like landfilling and incineration.
32178677	9	21	theme	productivity	1821:1832	arg1	species					1801:1807	Each species	1796:1807	Each species' growth and productivity were characterised	1796:1851	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	5	22	contain	contained	964:972	arg2	fraction					991:998	a polysaccharide fraction	974:998	a polysaccharide fraction comprising 38% cellulose and 4% hemicellulose	974:1044	RESULTS The autoclave pre-treated OMSW fibre contained a polysaccharide fraction comprising 38% cellulose and 4% hemicellulose.
32178677	5	22	contain	contained	964:972	arg1	RESULTS					919:925	RESULTS	919:925	RESULTS The autoclave pre-treated OMSW fibre	919:962	RESULTS The autoclave pre-treated OMSW fibre contained a polysaccharide fraction comprising 38% cellulose and 4% hemicellulose.
32178677	0	23	theme	municipal	63:71	arg1	waste					79:83	municipal solid waste	63:83	municipal solid waste	63:83	Robust microorganisms for biofuel and chemical production from municipal solid waste.
32178677	5	24	theme	fibre	958:962	arg1	RESULTS					919:925	RESULTS	919:925	RESULTS The autoclave pre-treated OMSW fibre	919:962	RESULTS The autoclave pre-treated OMSW fibre contained a polysaccharide fraction comprising 38% cellulose and 4% hemicellulose.
32178677	2	25	theme	heterogeneous	497:509	arg1	composition					511:521	its highly inconsistent and heterogeneous composition	469:521	its highly inconsistent and heterogeneous composition	469:521	The organic fraction of MSW (OMSW) typically comprises ~ 50% lignocellulose-rich material but is underexplored as a biomanufacturing feedstock due to its highly inconsistent and heterogeneous composition.
32178677	6	26	from	high	1087:1090	arg1	w/v					1134:1136	1.8%w/v	1130:1136	1.8%w/v	1130:1136	Enzymatic hydrolysate of OMSW fibre was high in D-glucose (5.5% w/v) and D-xylose (1.8%w/v) but deficient in nitrogen and phosphate.
32178677	6	26	from	high	1087:1090	arg1	w/v					1111:1113	5.5% w/v	1106:1113	5.5% w/v	1106:1113	Enzymatic hydrolysate of OMSW fibre was high in D-glucose (5.5% w/v) and D-xylose (1.8%w/v) but deficient in nitrogen and phosphate.
32178677	6	26	from	high	1087:1090	arg1	D-xylose					1120:1127	D-xylose	1120:1127	D-xylose (1.8%w/v)	1120:1137	Enzymatic hydrolysate of OMSW fibre was high in D-glucose (5.5% w/v) and D-xylose (1.8%w/v) but deficient in nitrogen and phosphate.
32178677	6	26	from	high	1087:1090	arg1	D-glucose					1095:1103	D-glucose	1095:1103	D-glucose (5.5% w/v)	1095:1114	Enzymatic hydrolysate of OMSW fibre was high in D-glucose (5.5% w/v) and D-xylose (1.8%w/v) but deficient in nitrogen and phosphate.
32178677	5	27	dep	%	1013:1013	arg1	cellulose					1015:1023	cellulose	1015:1023	38% cellulose	1011:1023	RESULTS The autoclave pre-treated OMSW fibre contained a polysaccharide fraction comprising 38% cellulose and 4% hemicellulose.
32178677	8	28	theme	shake-flask	1517:1527	arg1	screen					1529:1534	a substrate-oriented shake-flask screen	1496:1534	a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis)	1496:1793	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
32178677	6	29	from	D-glucose	1095:1103	arg1	high					1087:1090	high	1087:1090	high	1087:1090	Enzymatic hydrolysate of OMSW fibre was high in D-glucose (5.5% w/v) and D-xylose (1.8%w/v) but deficient in nitrogen and phosphate.
32178677	9	30	theme	maximum	2090:2096	arg1	yield					2123:2127	the maximum theoretical fermentation yield	2086:2127	the maximum theoretical fermentation yield	2086:2127	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	4	31	theme	biotechnological	820:835	arg1	interest					837:844	biotechnological interest	820:844	biotechnological interest	820:844	The resulting OMSW fibre was enzymatically hydrolysed and used to screen diverse microorganisms of biotechnological interest to identify robust species capable of fermenting this complex feedstock.
32178677	2	32	theme	material	400:407	arg1	fraction					331:338	The organic fraction	319:338	The organic fraction of MSW (OMSW) typically comprises ~ 50% lignocellulose-rich material	319:407	The organic fraction of MSW (OMSW) typically comprises ~ 50% lignocellulose-rich material but is underexplored as a biomanufacturing feedstock due to its highly inconsistent and heterogeneous composition.
32178677	7	33	theme	acid	1224:1227	arg1	levels					1204:1209	relatively low levels	1189:1209	relatively low levels of levulinic acid (30 mM) and vanillin (2 mM)	1189:1255	Although relatively low levels of levulinic acid (30 mM) and vanillin (2 mM) were detected and furfural and 5-hydroxymethylfurfural were absent, the hydrolysate contained an abundance of potentially toxic metals (0.6% w/v).
32178677	9	34	theme	OMSW	1927:1930	arg1	hydrolysate					1938:1948	OMSW fibre hydrolysate	1927:1948	OMSW fibre hydrolysate	1927:1948	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	9	35	theme	fermentation	2110:2121	arg1	yield					2123:2127	the maximum theoretical fermentation yield	2086:2127	the maximum theoretical fermentation yield	2086:2127	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	0	36	theme	Robust	0:5	arg1	microorganisms					7:20	Robust microorganisms	0:20	Robust microorganisms for biofuel and chemical production from municipal solid waste	0:83	Robust microorganisms for biofuel and chemical production from municipal solid waste.
32178677	1	37	theme	unsustainable	260:272	arg1	practices					274:282	unsustainable practices	260:282	unsustainable practices like landfilling and incineration	260:316	BACKGROUND Worldwide 3.4 billion tonnes of municipal solid waste (MSW) will be produced annually by 2050, however, current approaches to MSW management predominantly involve unsustainable practices like landfilling and incineration.
32178677	1	38	theme	Worldwide	97:105	arg1	tonnes					119:124	BACKGROUND Worldwide 3.4 billion tonnes	86:124	BACKGROUND Worldwide 3.4 billion tonnes of municipal solid waste (MSW)	86:155	BACKGROUND Worldwide 3.4 billion tonnes of municipal solid waste (MSW) will be produced annually by 2050, however, current approaches to MSW management predominantly involve unsustainable practices like landfilling and incineration.
32178677	2	39	theme	biomanufacturing	435:450	arg1	feedstock					452:460	a biomanufacturing feedstock	433:460	a biomanufacturing feedstock due to its highly inconsistent and heterogeneous composition	433:521	The organic fraction of MSW (OMSW) typically comprises ~ 50% lignocellulose-rich material but is underexplored as a biomanufacturing feedstock due to its highly inconsistent and heterogeneous composition.
32178677	11	40	from	growth	2548:2553	arg1	hydrolysates					2563:2574	OMSW hydrolysates	2558:2574	OMSW hydrolysates	2558:2574	Substrate-oriented screening of a representative and reproducible OMSW-derived fibre identified microorganisms intrinsically suited to growth on OMSW hydrolysates.
32178677	7	41	theme	low	1200:1202	arg1	levels					1204:1209	relatively low levels	1189:1209	relatively low levels of levulinic acid (30 mM) and vanillin (2 mM)	1189:1255	Although relatively low levels of levulinic acid (30 mM) and vanillin (2 mM) were detected and furfural and 5-hydroxymethylfurfural were absent, the hydrolysate contained an abundance of potentially toxic metals (0.6% w/v).
32178677	1	42	theme	billion	111:117	arg1	tonnes					119:124	BACKGROUND Worldwide 3.4 billion tonnes	86:124	BACKGROUND Worldwide 3.4 billion tonnes of municipal solid waste (MSW)	86:155	BACKGROUND Worldwide 3.4 billion tonnes of municipal solid waste (MSW) will be produced annually by 2050, however, current approaches to MSW management predominantly involve unsustainable practices like landfilling and incineration.
32178677	6	43	theme	%	1109:1109	arg1	w/v					1111:1113	5.5% w/v	1106:1113	5.5% w/v	1106:1113	Enzymatic hydrolysate of OMSW fibre was high in D-glucose (5.5% w/v) and D-xylose (1.8%w/v) but deficient in nitrogen and phosphate.
32178677	6	43	theme	%	1109:1109	arg1	D-glucose					1095:1103	D-glucose	1095:1103	D-glucose (5.5% w/v)	1095:1114	Enzymatic hydrolysate of OMSW fibre was high in D-glucose (5.5% w/v) and D-xylose (1.8%w/v) but deficient in nitrogen and phosphate.
32178677	2	44	theme	inconsistent	480:491	arg1	composition					511:521	its highly inconsistent and heterogeneous composition	469:521	its highly inconsistent and heterogeneous composition	469:521	The organic fraction of MSW (OMSW) typically comprises ~ 50% lignocellulose-rich material but is underexplored as a biomanufacturing feedstock due to its highly inconsistent and heterogeneous composition.
32178677	4	45	theme	capable	873:879	arg1	species					865:871	robust species	858:871	robust species capable of fermenting this complex feedstock	858:916	The resulting OMSW fibre was enzymatically hydrolysed and used to screen diverse microorganisms of biotechnological interest to identify robust species capable of fermenting this complex feedstock.
32178677	9	46	theme	fibre	1932:1936	arg1	hydrolysate					1938:1948	OMSW fibre hydrolysate	1927:1948	OMSW fibre hydrolysate	1927:1948	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	3	47	theme	MSW	675:677	arg1	mixture					679:685	a realistic and reproducible MSW mixture	646:685	a realistic and reproducible MSW mixture	646:685	This study sought to overcome the limitations associated with studying MSW-derived feedstocks by using OMSW produced from a realistic and reproducible MSW mixture on a commercial autoclave system.
32178677	4	48	theme	fermenting	884:893	arg1	feedstock					908:916	fermenting this complex feedstock	884:916	fermenting this complex feedstock	884:916	The resulting OMSW fibre was enzymatically hydrolysed and used to screen diverse microorganisms of biotechnological interest to identify robust species capable of fermenting this complex feedstock.
32178677	12	49	theme	future	2688:2693	arg1	studies					2695:2701	future studies	2688:2701	future studies aiming to valorise this underexplored feedstock	2688:2749	These species are promising candidates for developing an MSW biorefining platform and provide a foundation for future studies aiming to valorise this underexplored feedstock.
32178677	9	50	theme	significant	1958:1968	arg1	inhibition					1980:1989	significant substrate inhibition	1958:1989	significant substrate inhibition	1958:1989	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	8	51	theme	1	1434:1434	arg1	%					1435:1435	%	1435:1435	%	1435:1435	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
32178677	8	52	theme	Geobacillus	1647:1657	arg1	thermoglucosidasius					1659:1677	Geobacillus thermoglucosidasius	1647:1677	Geobacillus thermoglucosidasius	1647:1677	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
32178677	3	53	theme	commercial	692:701	arg1	system					713:718	a commercial autoclave system	690:718	a commercial autoclave system	690:718	This study sought to overcome the limitations associated with studying MSW-derived feedstocks by using OMSW produced from a realistic and reproducible MSW mixture on a commercial autoclave system.
32178677	8	54	theme	yeast	1437:1441	arg1	extract					1443:1449	1% yeast extract	1434:1449	1% yeast extract to alleviate nutrient limitation	1434:1482	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
32178677	9	55	theme	Z.	1992:1993	arg1	mobilis					1995:2001	Z. mobilis	1992:2001	Z. mobilis	1992:2001	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	9	55	theme	Z.	1992:1993	arg1	cerevisiae					2007:2016	S. cerevisiae	2004:2016	S. cerevisiae	2004:2016	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	9	55	theme	Z.	1992:1993	arg1	opacus					2025:2030	opacus	2025:2030	opacus	2025:2030	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	3	56	link	MSW-derived	595:605	arg1	feedstocks					607:616	MSW-derived feedstocks	595:616	MSW-derived feedstocks	595:616	This study sought to overcome the limitations associated with studying MSW-derived feedstocks by using OMSW produced from a realistic and reproducible MSW mixture on a commercial autoclave system.
32178677	1	57	theme	MSW	223:225	arg1	management					227:236	MSW management	223:236	MSW management	223:236	BACKGROUND Worldwide 3.4 billion tonnes of municipal solid waste (MSW) will be produced annually by 2050, however, current approaches to MSW management predominantly involve unsustainable practices like landfilling and incineration.
32178677	10	58	theme	environmental	2353:2365	arg1	burden					2367:2372	the environmental burden	2349:2372	the environmental burden of current waste management practices	2349:2410	CONCLUSIONS Developing an integrated biorefinery around MSW has the potential to significantly alleviate the environmental burden of current waste management practices.
32178677	9	59	theme	triacylglycerol	2203:2217	arg1	139 kg					2172:2177	139 kg	2172:2177	139 kg	2172:2177	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	9	59	theme	triacylglycerol	2203:2217	arg1	136 kg					2161:2166	136 kg	2161:2166	136 kg	2161:2166	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	4	60	used	used	779:782	arg2	OMSW					735:738	The resulting OMSW fibre	721:744	The resulting OMSW fibre	721:744	The resulting OMSW fibre was enzymatically hydrolysed and used to screen diverse microorganisms of biotechnological interest to identify robust species capable of fermenting this complex feedstock.
32178677	12	61	theme	MSW	2634:2636	arg1	platform					2650:2657	an MSW biorefining platform	2631:2657	an MSW biorefining platform	2631:2657	These species are promising candidates for developing an MSW biorefining platform and provide a foundation for future studies aiming to valorise this underexplored feedstock.
32178677	8	62	theme	Rhodococcus	1700:1710	arg1	opacus					1712:1717	Rhodococcus opacus	1700:1717	Rhodococcus opacus	1700:1717	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
32178677	6	63	from	D-xylose	1120:1127	arg1	high					1087:1090	high	1087:1090	high	1087:1090	Enzymatic hydrolysate of OMSW fibre was high in D-glucose (5.5% w/v) and D-xylose (1.8%w/v) but deficient in nitrogen and phosphate.
32178677	10	64	theme	waste	2385:2389	arg1	practices					2402:2410	current waste management practices	2377:2410	current waste management practices	2377:2410	CONCLUSIONS Developing an integrated biorefinery around MSW has the potential to significantly alleviate the environmental burden of current waste management practices.
32178677	3	65	theme	MSW-derived	595:605	arg1	feedstocks					607:616	MSW-derived feedstocks	595:616	MSW-derived feedstocks	595:616	This study sought to overcome the limitations associated with studying MSW-derived feedstocks by using OMSW produced from a realistic and reproducible MSW mixture on a commercial autoclave system.
32178677	5	66	theme	autoclave	931:939	arg1	OMSW					953:956	The autoclave pre-treated OMSW	927:956	The autoclave pre-treated OMSW	927:956	RESULTS The autoclave pre-treated OMSW fibre contained a polysaccharide fraction comprising 38% cellulose and 4% hemicellulose.
32178677	6	67	theme	Enzymatic	1047:1055	arg1	hydrolysate					1057:1067	Enzymatic hydrolysate	1047:1067	Enzymatic hydrolysate of OMSW fibre	1047:1081	Enzymatic hydrolysate of OMSW fibre was high in D-glucose (5.5% w/v) and D-xylose (1.8%w/v) but deficient in nitrogen and phosphate.
32178677	4	68	theme	feedstock	908:916	arg1	capable					873:879	capable	873:879	capable	873:879	The resulting OMSW fibre was enzymatically hydrolysed and used to screen diverse microorganisms of biotechnological interest to identify robust species capable of fermenting this complex feedstock.
32178677	11	69	theme	Substrate-oriented	2413:2430	arg1	screening					2432:2440	Substrate-oriented screening	2413:2440	Substrate-oriented screening of a representative and reproducible OMSW-derived fibre	2413:2496	Substrate-oriented screening of a representative and reproducible OMSW-derived fibre identified microorganisms intrinsically suited to growth on OMSW hydrolysates.
32178677	10	70	theme	practices	2402:2410	arg1	burden					2367:2372	the environmental burden	2349:2372	the environmental burden of current waste management practices	2349:2410	CONCLUSIONS Developing an integrated biorefinery around MSW has the potential to significantly alleviate the environmental burden of current waste management practices.
32178677	8	71	theme	nutrient	1464:1471	arg1	limitation					1473:1482	nutrient limitation	1464:1482	nutrient limitation	1464:1482	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
32178677	11	72	theme	OMSW	2558:2561	arg1	hydrolysates					2563:2574	OMSW hydrolysates	2558:2574	OMSW hydrolysates	2558:2574	Substrate-oriented screening of a representative and reproducible OMSW-derived fibre identified microorganisms intrinsically suited to growth on OMSW hydrolysates.
32178677	5	73	theme	polysaccharide	976:989	arg1	fraction					991:998	a polysaccharide fraction	974:998	a polysaccharide fraction comprising 38% cellulose and 4% hemicellulose	974:1044	RESULTS The autoclave pre-treated OMSW fibre contained a polysaccharide fraction comprising 38% cellulose and 4% hemicellulose.
32178677	4	74	theme	fibre	740:744	arg1	OMSW					735:738	The resulting OMSW fibre	721:744	The resulting OMSW fibre	721:744	The resulting OMSW fibre was enzymatically hydrolysed and used to screen diverse microorganisms of biotechnological interest to identify robust species capable of fermenting this complex feedstock.
32178677	2	75	dep	%	378:378	arg1	OMSW					348:351	OMSW	348:351	OMSW	348:351	The organic fraction of MSW (OMSW) typically comprises ~ 50% lignocellulose-rich material but is underexplored as a biomanufacturing feedstock due to its highly inconsistent and heterogeneous composition.
32178677	2	75	dep	%	378:378	arg1	comprises ~ 50					364:377	comprises ~ 50	364:377	comprises ~ 50	364:377	The organic fraction of MSW (OMSW) typically comprises ~ 50% lignocellulose-rich material but is underexplored as a biomanufacturing feedstock due to its highly inconsistent and heterogeneous composition.
32178677	2	75	dep	%	378:378	arg1	MSW					343:345	MSW	343:345	MSW	343:345	The organic fraction of MSW (OMSW) typically comprises ~ 50% lignocellulose-rich material but is underexplored as a biomanufacturing feedstock due to its highly inconsistent and heterogeneous composition.
32178677	3	76	theme	realistic	648:656	arg1	mixture					679:685	a realistic and reproducible MSW mixture	646:685	a realistic and reproducible MSW mixture	646:685	This study sought to overcome the limitations associated with studying MSW-derived feedstocks by using OMSW produced from a realistic and reproducible MSW mixture on a commercial autoclave system.
32178677	1	77	theme	municipal	129:137	arg1	MSW					152:154	MSW	152:154	MSW	152:154	BACKGROUND Worldwide 3.4 billion tonnes of municipal solid waste (MSW) will be produced annually by 2050, however, current approaches to MSW management predominantly involve unsustainable practices like landfilling and incineration.
32178677	1	77	theme	municipal	129:137	arg1	waste					145:149	municipal solid waste	129:149	municipal solid waste (MSW)	129:155	BACKGROUND Worldwide 3.4 billion tonnes of municipal solid waste (MSW) will be produced annually by 2050, however, current approaches to MSW management predominantly involve unsustainable practices like landfilling and incineration.
32178677	7	78	theme	metals	1385:1390	arg1	abundance					1354:1362	an abundance	1351:1362	an abundance of potentially toxic metals (0.6% w/v)	1351:1401	Although relatively low levels of levulinic acid (30 mM) and vanillin (2 mM) were detected and furfural and 5-hydroxymethylfurfural were absent, the hydrolysate contained an abundance of potentially toxic metals (0.6% w/v).
32178677	10	79	theme	integrated	2270:2279	arg1	biorefinery					2281:2291	an integrated biorefinery	2267:2291	an integrated biorefinery around MSW	2267:2302	CONCLUSIONS Developing an integrated biorefinery around MSW has the potential to significantly alleviate the environmental burden of current waste management practices.
32178677	7	80	theme	vanillin	1241:1248	arg1	levels					1204:1209	relatively low levels	1189:1209	relatively low levels of levulinic acid (30 mM) and vanillin (2 mM)	1189:1255	Although relatively low levels of levulinic acid (30 mM) and vanillin (2 mM) were detected and furfural and 5-hydroxymethylfurfural were absent, the hydrolysate contained an abundance of potentially toxic metals (0.6% w/v).
32178677	8	81	dep	microorganisms	1573:1586	arg1	cerevisiae					1734:1743	Saccharomyces cerevisiae	1720:1743	Saccharomyces cerevisiae	1720:1743	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
32178677	8	81	dep	microorganisms	1573:1586	arg1	mobilis					1786:1792	Zymomonas mobilis	1776:1792	Zymomonas mobilis	1776:1792	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
32178677	8	81	dep	microorganisms	1573:1586	arg1	coli					1641:1644	Escherichia coli	1629:1644	Escherichia coli	1629:1644	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
32178677	8	81	dep	microorganisms	1573:1586	arg1	putida					1692:1697	Pseudomonas putida	1680:1697	Pseudomonas putida	1680:1697	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
32178677	8	81	dep	microorganisms	1573:1586	arg1	opacus					1712:1717	Rhodococcus opacus	1700:1717	Rhodococcus opacus	1700:1717	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
32178677	8	81	dep	microorganisms	1573:1586	arg1	pombe					1766:1770	Schizosaccharomyces pombe	1746:1770	Schizosaccharomyces pombe	1746:1770	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
32178677	8	81	dep	microorganisms	1573:1586	arg1	saccharoperbutylacetonicum					1601:1626	Clostridium saccharoperbutylacetonicum	1589:1626	Clostridium saccharoperbutylacetonicum	1589:1626	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
32178677	8	81	dep	microorganisms	1573:1586	arg1	thermoglucosidasius					1659:1677	Geobacillus thermoglucosidasius	1647:1677	Geobacillus thermoglucosidasius	1647:1677	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
32178677	1	82	theme	waste	145:149	arg1	tonnes					119:124	BACKGROUND Worldwide 3.4 billion tonnes	86:124	BACKGROUND Worldwide 3.4 billion tonnes of municipal solid waste (MSW)	86:155	BACKGROUND Worldwide 3.4 billion tonnes of municipal solid waste (MSW) will be produced annually by 2050, however, current approaches to MSW management predominantly involve unsustainable practices like landfilling and incineration.
32178677	9	83	theme	were characterised	1834:1851	arg1	species					1801:1807	Each species	1796:1807	Each species' growth and productivity were characterised	1796:1851	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	8	84	theme	substrate-oriented	1498:1515	arg1	screen					1529:1534	a substrate-oriented shake-flask screen	1496:1534	a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis)	1496:1793	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
32178677	10	85	contain	has	2304:2306	arg2	potential					2312:2320	the potential to significantly alleviate the environmental burden of current waste management practices	2308:2410	the potential to significantly alleviate the environmental burden of current waste management practices	2308:2410	CONCLUSIONS Developing an integrated biorefinery around MSW has the potential to significantly alleviate the environmental burden of current waste management practices.
32178677	10	85	contain	has	2304:2306	arg1	CONCLUSIONS					2244:2254	CONCLUSIONS	2244:2254	CONCLUSIONS Developing an integrated biorefinery around MSW	2244:2302	CONCLUSIONS Developing an integrated biorefinery around MSW has the potential to significantly alleviate the environmental burden of current waste management practices.
32178677	6	86	theme	fibre	1077:1081	arg1	hydrolysate					1057:1067	Enzymatic hydrolysate	1047:1067	Enzymatic hydrolysate of OMSW fibre	1047:1081	Enzymatic hydrolysate of OMSW fibre was high in D-glucose (5.5% w/v) and D-xylose (1.8%w/v) but deficient in nitrogen and phosphate.
32178677	11	87	theme	fibre	2492:2496	arg1	screening					2432:2440	Substrate-oriented screening	2413:2440	Substrate-oriented screening of a representative and reproducible OMSW-derived fibre	2413:2496	Substrate-oriented screening of a representative and reproducible OMSW-derived fibre identified microorganisms intrinsically suited to growth on OMSW hydrolysates.
32178677	4	88	theme	resulting	725:733	arg1	OMSW					735:738	The resulting OMSW fibre	721:744	The resulting OMSW fibre	721:744	The resulting OMSW fibre was enzymatically hydrolysed and used to screen diverse microorganisms of biotechnological interest to identify robust species capable of fermenting this complex feedstock.
32178677	11	89	link	OMSW-derived	2479:2490	arg1	fibre					2492:2496	fibre	2492:2496	fibre	2492:2496	Substrate-oriented screening of a representative and reproducible OMSW-derived fibre identified microorganisms intrinsically suited to growth on OMSW hydrolysates.
32178677	4	90	theme	diverse	794:800	arg1	microorganisms					802:815	diverse microorganisms	794:815	diverse microorganisms of biotechnological interest	794:844	The resulting OMSW fibre was enzymatically hydrolysed and used to screen diverse microorganisms of biotechnological interest to identify robust species capable of fermenting this complex feedstock.
32178677	7	91	theme	levulinic	1214:1222	arg1	30 mM					1230:1234	30 mM	1230:1234	30 mM	1230:1234	Although relatively low levels of levulinic acid (30 mM) and vanillin (2 mM) were detected and furfural and 5-hydroxymethylfurfural were absent, the hydrolysate contained an abundance of potentially toxic metals (0.6% w/v).
32178677	7	91	theme	levulinic	1214:1222	arg1	acid					1224:1227	levulinic acid	1214:1227	levulinic acid (30 mM)	1214:1235	Although relatively low levels of levulinic acid (30 mM) and vanillin (2 mM) were detected and furfural and 5-hydroxymethylfurfural were absent, the hydrolysate contained an abundance of potentially toxic metals (0.6% w/v).
32178677	8	92	theme	useful	1566:1571	arg1	microorganisms					1573:1586	eight biotechnologically useful microorganisms	1541:1586	eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis)	1541:1793	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
32178677	9	93	theme	theoretical	2098:2108	arg1	yield					2123:2127	the maximum theoretical fermentation yield	2086:2127	the maximum theoretical fermentation yield	2086:2127	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	2	94	theme	lignocellulose-rich	380:398	arg1	material					400:407	MSW (OMSW) typically comprises ~ 50% lignocellulose-rich material	343:407	MSW (OMSW) typically comprises ~ 50% lignocellulose-rich material	343:407	The organic fraction of MSW (OMSW) typically comprises ~ 50% lignocellulose-rich material but is underexplored as a biomanufacturing feedstock due to its highly inconsistent and heterogeneous composition.
32178677	1	95	theme	BACKGROUND	86:95	arg1	tonnes					119:124	BACKGROUND Worldwide 3.4 billion tonnes	86:124	BACKGROUND Worldwide 3.4 billion tonnes of municipal solid waste (MSW)	86:155	BACKGROUND Worldwide 3.4 billion tonnes of municipal solid waste (MSW) will be produced annually by 2050, however, current approaches to MSW management predominantly involve unsustainable practices like landfilling and incineration.
32178677	9	96	theme	ethanol	2182:2188	arg1	139 kg					2172:2177	139 kg	2172:2177	139 kg	2172:2177	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	9	96	theme	ethanol	2182:2188	arg1	136 kg					2161:2166	136 kg	2161:2166	136 kg	2161:2166	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	9	97	theme	yield	2123:2127	arg1	%					2068:2068	69%	2066:2068	69%	2066:2068	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	9	97	theme	yield	2123:2127	arg1	%					2073:2073	70%	2071:2073	70%	2071:2073	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	9	97	theme	yield	2123:2127	arg1	%					2081:2081	72%	2079:2081	72%	2079:2081	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	9	97	theme	yield	2123:2127	arg1	yield					2123:2127	the maximum theoretical fermentation yield	2086:2127	the maximum theoretical fermentation yield	2086:2127	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	6	98	from	phosphate	1169:1177	arg1	deficient					1143:1151	deficient	1143:1151	deficient	1143:1151	Enzymatic hydrolysate of OMSW fibre was high in D-glucose (5.5% w/v) and D-xylose (1.8%w/v) but deficient in nitrogen and phosphate.
32178677	0	99	theme	biofuel	26:32	arg1	production					47:56	biofuel and chemical production	26:56	biofuel and chemical production from municipal solid waste	26:83	Robust microorganisms for biofuel and chemical production from municipal solid waste.
32178677	4	100	theme	robust	858:863	arg1	species					865:871	robust species	858:871	robust species capable of fermenting this complex feedstock	858:916	The resulting OMSW fibre was enzymatically hydrolysed and used to screen diverse microorganisms of biotechnological interest to identify robust species capable of fermenting this complex feedstock.
32178677	0	101	theme	chemical	38:45	arg1	production					47:56	biofuel and chemical production	26:56	biofuel and chemical production from municipal solid waste	26:83	Robust microorganisms for biofuel and chemical production from municipal solid waste.
32178677	8	102	with	screen	1529:1534	arg1	microorganisms					1573:1586	eight biotechnologically useful microorganisms	1541:1586	eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis)	1541:1793	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
32178677	3	103	theme	reproducible	662:673	arg1	mixture					679:685	a realistic and reproducible MSW mixture	646:685	a realistic and reproducible MSW mixture	646:685	This study sought to overcome the limitations associated with studying MSW-derived feedstocks by using OMSW produced from a realistic and reproducible MSW mixture on a commercial autoclave system.
32178677	6	104	from	deficient	1143:1151	arg1	phosphate					1169:1177	phosphate	1169:1177	phosphate	1169:1177	Enzymatic hydrolysate of OMSW fibre was high in D-glucose (5.5% w/v) and D-xylose (1.8%w/v) but deficient in nitrogen and phosphate.
32178677	6	104	from	deficient	1143:1151	arg1	nitrogen					1156:1163	nitrogen	1156:1163	nitrogen	1156:1163	Enzymatic hydrolysate of OMSW fibre was high in D-glucose (5.5% w/v) and D-xylose (1.8%w/v) but deficient in nitrogen and phosphate.
32178677	3	105	theme	autoclave	703:711	arg1	system					713:718	a commercial autoclave system	690:718	a commercial autoclave system	690:718	This study sought to overcome the limitations associated with studying MSW-derived feedstocks by using OMSW produced from a realistic and reproducible MSW mixture on a commercial autoclave system.
32178677	6	106	from	nitrogen	1156:1163	arg1	deficient					1143:1151	deficient	1143:1151	deficient	1143:1151	Enzymatic hydrolysate of OMSW fibre was high in D-glucose (5.5% w/v) and D-xylose (1.8%w/v) but deficient in nitrogen and phosphate.
32178677	8	107	dep	extract	1443:1449	arg1	alleviate					1454:1462	alleviate	1454:1462	to alleviate nutrient limitation	1451:1482	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
32178677	9	108	theme	substrate	1970:1978	arg1	inhibition					1980:1989	significant substrate inhibition	1958:1989	significant substrate inhibition	1958:1989	Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW.
32178677	4	109	theme	interest	837:844	arg1	microorganisms					802:815	diverse microorganisms	794:815	diverse microorganisms of biotechnological interest	794:844	The resulting OMSW fibre was enzymatically hydrolysed and used to screen diverse microorganisms of biotechnological interest to identify robust species capable of fermenting this complex feedstock.
32178677	2	110	theme	organic	323:329	arg1	fraction					331:338	The organic fraction	319:338	The organic fraction of MSW (OMSW) typically comprises ~ 50% lignocellulose-rich material	319:407	The organic fraction of MSW (OMSW) typically comprises ~ 50% lignocellulose-rich material but is underexplored as a biomanufacturing feedstock due to its highly inconsistent and heterogeneous composition.
32178677	1	111	theme	current	201:207	arg1	approaches					209:218	current approaches	201:218	current approaches to MSW management	201:236	BACKGROUND Worldwide 3.4 billion tonnes of municipal solid waste (MSW) will be produced annually by 2050, however, current approaches to MSW management predominantly involve unsustainable practices like landfilling and incineration.
32178677	8	112	theme	%	1435:1435	arg1	extract					1443:1449	1% yeast extract	1434:1449	1% yeast extract to alleviate nutrient limitation	1434:1482	Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis).
34498470	2	0	theme	polysaccharide	402:415	arg1	fractions					417:425	eight different S. japonica polysaccharide fractions	374:425	eight different S. japonica polysaccharide fractions	374:425	In the current study, we investigated the impact of eight different S. japonica polysaccharide fractions on the gut microbiota after day 2 and day 14 treatments on high-fat diet (HFD) feeding mice.
34498470	6	1	theme	microbiota	1043:1052	arg1	homeostasis					1054:1064	gut microbiota homeostasis	1039:1064	gut microbiota homeostasis	1039:1064	Our findings reveal that gut microbiota homeostasis could be disordered by HFD at day 2 and provide insights into the quantitative composition-activity relationships of polysaccharides in regulating gut microbiota.
34498470	5	2	theme	main	956:959	arg1	content					900:906	the sulfate content	888:906	the sulfate content	888:906	Multiple linear regression analysis revealed that the sulfate content and the molecular weight distributions were the main factors affecting the dominant gut bacterial genera.
34498470	5	2	theme	main	956:959	arg1	factors					961:967	the main factors	952:967	the main factors affecting the dominant gut bacterial genera	952:1011	Multiple linear regression analysis revealed that the sulfate content and the molecular weight distributions were the main factors affecting the dominant gut bacterial genera.
34498470	5	2	theme	main	956:959	arg1	weight					926:931	the molecular weight	912:931	the molecular weight	912:931	Multiple linear regression analysis revealed that the sulfate content and the molecular weight distributions were the main factors affecting the dominant gut bacterial genera.
34498470	0	3	from	Relationships	21:33	arg1	Saccharina					59:68	Saccharina	59:68	Saccharina	59:68	Composition-Activity Relationships of Polysaccharides from Saccharina japonica in Regulating Gut Microbiota in Short-Term High-Fat Diet-Fed Mice.
34498470	1	4	theme	microbiota	200:209	arg1	composition					211:221	gut microbiota composition	196:221	gut microbiota composition	196:221	Saccharina japonica polysaccharide could modulate gut microbiota composition; however, the composition-activity relationship remains unclear, thus restricting its application.
34498470	2	5	from	impact	364:369	arg1	microbiota					438:447	the gut microbiota	430:447	the gut microbiota	430:447	In the current study, we investigated the impact of eight different S. japonica polysaccharide fractions on the gut microbiota after day 2 and day 14 treatments on high-fat diet (HFD) feeding mice.
34498470	6	6	theme	gut	1039:1041	arg1	homeostasis					1054:1064	gut microbiota homeostasis	1039:1064	gut microbiota homeostasis	1039:1064	Our findings reveal that gut microbiota homeostasis could be disordered by HFD at day 2 and provide insights into the quantitative composition-activity relationships of polysaccharides in regulating gut microbiota.
34498470	0	7	theme	High-Fat	122:129	arg1	Mice					140:143	Short-Term High-Fat Diet-Fed Mice	111:143	Short-Term High-Fat Diet-Fed Mice	111:143	Composition-Activity Relationships of Polysaccharides from Saccharina japonica in Regulating Gut Microbiota in Short-Term High-Fat Diet-Fed Mice.
34498470	4	8	theme	composition	777:787	arg1	dysbiosis					749:757	the dysbiosis	745:757	the dysbiosis of gut microbiota composition	745:787	LjA-1 and LjA-3 could partially alleviate the dysbiosis of gut microbiota composition and significantly alter gut microbiota function.
34498470	3	9	theme	gut	577:579	arg1	composition					592:602	gut microbiota composition	577:602	gut microbiota composition	577:602	The results showed that a 2 day HFD dramatically altered gut microbiota composition, and the additional 12 day HFD further strengthened the gut microbiota dysbiosis in the HFD group.
34498470	0	10	theme	Short-Term	111:120	arg1	Mice					140:143	Short-Term High-Fat Diet-Fed Mice	111:143	Short-Term High-Fat Diet-Fed Mice	111:143	Composition-Activity Relationships of Polysaccharides from Saccharina japonica in Regulating Gut Microbiota in Short-Term High-Fat Diet-Fed Mice.
34498470	4	11	theme	microbiota	766:775	arg1	composition					777:787	gut microbiota composition	762:787	gut microbiota composition	762:787	LjA-1 and LjA-3 could partially alleviate the dysbiosis of gut microbiota composition and significantly alter gut microbiota function.
34498470	1	12	theme	gut	196:198	arg1	composition					211:221	gut microbiota composition	196:221	gut microbiota composition	196:221	Saccharina japonica polysaccharide could modulate gut microbiota composition; however, the composition-activity relationship remains unclear, thus restricting its application.
34498470	2	13	theme	current	329:335	arg1	study					337:341	the current study	325:341	the current study	325:341	In the current study, we investigated the impact of eight different S. japonica polysaccharide fractions on the gut microbiota after day 2 and day 14 treatments on high-fat diet (HFD) feeding mice.
34498470	5	14	theme	sulfate	892:898	arg1	content					900:906	the sulfate content	888:906	the sulfate content	888:906	Multiple linear regression analysis revealed that the sulfate content and the molecular weight distributions were the main factors affecting the dominant gut bacterial genera.
34498470	5	14	theme	sulfate	892:898	arg1	factors					961:967	the main factors	952:967	the main factors affecting the dominant gut bacterial genera	952:1011	Multiple linear regression analysis revealed that the sulfate content and the molecular weight distributions were the main factors affecting the dominant gut bacterial genera.
34498470	5	14	theme	sulfate	892:898	arg1	weight					926:931	the molecular weight	912:931	the molecular weight	912:931	Multiple linear regression analysis revealed that the sulfate content and the molecular weight distributions were the main factors affecting the dominant gut bacterial genera.
34498470	0	15	theme	Diet-Fed	131:138	arg1	Mice					140:143	Short-Term High-Fat Diet-Fed Mice	111:143	Short-Term High-Fat Diet-Fed Mice	111:143	Composition-Activity Relationships of Polysaccharides from Saccharina japonica in Regulating Gut Microbiota in Short-Term High-Fat Diet-Fed Mice.
34498470	0	16	from	Saccharina	59:68	arg1	Polysaccharides					38:52	Polysaccharides	38:52	Polysaccharides from Saccharina japonica in	38:80	Composition-Activity Relationships of Polysaccharides from Saccharina japonica in Regulating Gut Microbiota in Short-Term High-Fat Diet-Fed Mice.
34498470	0	16	from	Saccharina	59:68	arg1	Relationships					21:33	Composition-Activity Relationships	0:33	Composition-Activity Relationships of Polysaccharides from Saccharina japonica in	0:80	Composition-Activity Relationships of Polysaccharides from Saccharina japonica in Regulating Gut Microbiota in Short-Term High-Fat Diet-Fed Mice.
34498470	6	17	theme	gut	1213:1215	arg1	microbiota					1217:1226	gut microbiota	1213:1226	gut microbiota	1213:1226	Our findings reveal that gut microbiota homeostasis could be disordered by HFD at day 2 and provide insights into the quantitative composition-activity relationships of polysaccharides in regulating gut microbiota.
34498470	3	18	theme	day	548:550	arg1	HFD					552:554	a 2 day HFD	544:554	a 2 day HFD	544:554	The results showed that a 2 day HFD dramatically altered gut microbiota composition, and the additional 12 day HFD further strengthened the gut microbiota dysbiosis in the HFD group.
34498470	2	19	theme	high-fat	486:493	arg1	HFD					501:503	HFD	501:503	HFD	501:503	In the current study, we investigated the impact of eight different S. japonica polysaccharide fractions on the gut microbiota after day 2 and day 14 treatments on high-fat diet (HFD) feeding mice.
34498470	2	19	theme	high-fat	486:493	arg1	diet					495:498	high-fat diet	486:498	high-fat diet (HFD) feeding mice	486:517	In the current study, we investigated the impact of eight different S. japonica polysaccharide fractions on the gut microbiota after day 2 and day 14 treatments on high-fat diet (HFD) feeding mice.
34498470	3	20	theme	microbiota	581:590	arg1	composition					592:602	gut microbiota composition	577:602	gut microbiota composition	577:602	The results showed that a 2 day HFD dramatically altered gut microbiota composition, and the additional 12 day HFD further strengthened the gut microbiota dysbiosis in the HFD group.
34498470	2	21	theme	gut	434:436	arg1	microbiota					438:447	the gut microbiota	430:447	the gut microbiota	430:447	In the current study, we investigated the impact of eight different S. japonica polysaccharide fractions on the gut microbiota after day 2 and day 14 treatments on high-fat diet (HFD) feeding mice.
34498470	0	22	theme	Composition-Activity	0:19	arg1	Relationships					21:33	Composition-Activity Relationships	0:33	Composition-Activity Relationships of Polysaccharides from Saccharina japonica in	0:80	Composition-Activity Relationships of Polysaccharides from Saccharina japonica in Regulating Gut Microbiota in Short-Term High-Fat Diet-Fed Mice.
34498470	6	23	from	day	1096:1098	arg1	HFD					1089:1091	HFD	1089:1091	HFD at day 2	1089:1100	Our findings reveal that gut microbiota homeostasis could be disordered by HFD at day 2 and provide insights into the quantitative composition-activity relationships of polysaccharides in regulating gut microbiota.
34498470	5	24	theme	dominant	983:990	arg1	genera					1006:1011	the dominant gut bacterial genera	979:1011	the dominant gut bacterial genera	979:1011	Multiple linear regression analysis revealed that the sulfate content and the molecular weight distributions were the main factors affecting the dominant gut bacterial genera.
34498470	3	25	theme	microbiota	664:673	arg1	dysbiosis					675:683	the gut microbiota dysbiosis	656:683	the gut microbiota dysbiosis	656:683	The results showed that a 2 day HFD dramatically altered gut microbiota composition, and the additional 12 day HFD further strengthened the gut microbiota dysbiosis in the HFD group.
34498470	0	26	theme	Polysaccharides	38:52	arg1	Relationships					21:33	Composition-Activity Relationships	0:33	Composition-Activity Relationships of Polysaccharides from Saccharina japonica in	0:80	Composition-Activity Relationships of Polysaccharides from Saccharina japonica in Regulating Gut Microbiota in Short-Term High-Fat Diet-Fed Mice.
34498470	5	27	theme	Multiple	838:845	arg1	analysis					865:872	Multiple linear regression analysis	838:872	Multiple linear regression analysis	838:872	Multiple linear regression analysis revealed that the sulfate content and the molecular weight distributions were the main factors affecting the dominant gut bacterial genera.
34498470	4	28	theme	gut	762:764	arg1	composition					777:787	gut microbiota composition	762:787	gut microbiota composition	762:787	LjA-1 and LjA-3 could partially alleviate the dysbiosis of gut microbiota composition and significantly alter gut microbiota function.
34498470	5	29	theme	bacterial	996:1004	arg1	genera					1006:1011	the dominant gut bacterial genera	979:1011	the dominant gut bacterial genera	979:1011	Multiple linear regression analysis revealed that the sulfate content and the molecular weight distributions were the main factors affecting the dominant gut bacterial genera.
34498470	5	30	theme	regression	854:863	arg1	analysis					865:872	Multiple linear regression analysis	838:872	Multiple linear regression analysis	838:872	Multiple linear regression analysis revealed that the sulfate content and the molecular weight distributions were the main factors affecting the dominant gut bacterial genera.
34498470	3	31	theme	gut	660:662	arg1	dysbiosis					675:683	the gut microbiota dysbiosis	656:683	the gut microbiota dysbiosis	656:683	The results showed that a 2 day HFD dramatically altered gut microbiota composition, and the additional 12 day HFD further strengthened the gut microbiota dysbiosis in the HFD group.
34498470	2	32	theme	japonica	393:400	arg1	fractions					417:425	eight different S. japonica polysaccharide fractions	374:425	eight different S. japonica polysaccharide fractions	374:425	In the current study, we investigated the impact of eight different S. japonica polysaccharide fractions on the gut microbiota after day 2 and day 14 treatments on high-fat diet (HFD) feeding mice.
34498470	1	33	theme	composition-activity	237:256	arg1	relationship					258:269	the composition-activity relationship	233:269	the composition-activity relationship	233:269	Saccharina japonica polysaccharide could modulate gut microbiota composition; however, the composition-activity relationship remains unclear, thus restricting its application.
34498470	2	34	theme	different	380:388	arg1	fractions					417:425	eight different S. japonica polysaccharide fractions	374:425	eight different S. japonica polysaccharide fractions	374:425	In the current study, we investigated the impact of eight different S. japonica polysaccharide fractions on the gut microbiota after day 2 and day 14 treatments on high-fat diet (HFD) feeding mice.
34498470	0	35	from	Microbiota	97:106	arg1	Mice					140:143	Short-Term High-Fat Diet-Fed Mice	111:143	Short-Term High-Fat Diet-Fed Mice	111:143	Composition-Activity Relationships of Polysaccharides from Saccharina japonica in Regulating Gut Microbiota in Short-Term High-Fat Diet-Fed Mice.
34498470	2	36	dep	day	455:457	arg1	treatments					472:481	treatments	472:481	treatments	472:481	In the current study, we investigated the impact of eight different S. japonica polysaccharide fractions on the gut microbiota after day 2 and day 14 treatments on high-fat diet (HFD) feeding mice.
34498470	5	37	theme	linear	847:852	arg1	analysis					865:872	Multiple linear regression analysis	838:872	Multiple linear regression analysis	838:872	Multiple linear regression analysis revealed that the sulfate content and the molecular weight distributions were the main factors affecting the dominant gut bacterial genera.
34498470	0	38	dep	Saccharina	59:68	arg1	in					79:80	Saccharina japonica in	59:80	Saccharina japonica in	59:80	Composition-Activity Relationships of Polysaccharides from Saccharina japonica in Regulating Gut Microbiota in Short-Term High-Fat Diet-Fed Mice.
34498470	6	39	theme	polysaccharides	1183:1197	arg1	relationships					1166:1178	the quantitative composition-activity relationships	1128:1178	the quantitative composition-activity relationships of polysaccharides in regulating gut microbiota	1128:1226	Our findings reveal that gut microbiota homeostasis could be disordered by HFD at day 2 and provide insights into the quantitative composition-activity relationships of polysaccharides in regulating gut microbiota.
34498470	2	40	theme	S.	390:391	arg1	fractions					417:425	eight different S. japonica polysaccharide fractions	374:425	eight different S. japonica polysaccharide fractions	374:425	In the current study, we investigated the impact of eight different S. japonica polysaccharide fractions on the gut microbiota after day 2 and day 14 treatments on high-fat diet (HFD) feeding mice.
34498470	5	41	dep	content	900:906	arg1	distributions					933:945	distributions	933:945	distributions	933:945	Multiple linear regression analysis revealed that the sulfate content and the molecular weight distributions were the main factors affecting the dominant gut bacterial genera.
34498470	1	42	theme	Saccharina	146:155	arg1	polysaccharide					166:179	Saccharina japonica polysaccharide	146:179	Saccharina japonica polysaccharide	146:179	Saccharina japonica polysaccharide could modulate gut microbiota composition; however, the composition-activity relationship remains unclear, thus restricting its application.
34498470	4	43	theme	microbiota	817:826	arg1	function					828:835	gut microbiota function	813:835	gut microbiota function	813:835	LjA-1 and LjA-3 could partially alleviate the dysbiosis of gut microbiota composition and significantly alter gut microbiota function.
34498470	3	44	theme	HFD	692:694	arg1	group					696:700	the HFD group	688:700	the HFD group	688:700	The results showed that a 2 day HFD dramatically altered gut microbiota composition, and the additional 12 day HFD further strengthened the gut microbiota dysbiosis in the HFD group.
34498470	4	45	theme	gut	813:815	arg1	function					828:835	gut microbiota function	813:835	gut microbiota function	813:835	LjA-1 and LjA-3 could partially alleviate the dysbiosis of gut microbiota composition and significantly alter gut microbiota function.
34498470	3	46	theme	additional	613:622	arg1	HFD					631:633	the additional 12 day HFD	609:633	the additional 12 day HFD	609:633	The results showed that a 2 day HFD dramatically altered gut microbiota composition, and the additional 12 day HFD further strengthened the gut microbiota dysbiosis in the HFD group.
34498470	1	47	theme	japonica	157:164	arg1	polysaccharide					166:179	Saccharina japonica polysaccharide	146:179	Saccharina japonica polysaccharide	146:179	Saccharina japonica polysaccharide could modulate gut microbiota composition; however, the composition-activity relationship remains unclear, thus restricting its application.
34498470	5	48	theme	gut	992:994	arg1	genera					1006:1011	the dominant gut bacterial genera	979:1011	the dominant gut bacterial genera	979:1011	Multiple linear regression analysis revealed that the sulfate content and the molecular weight distributions were the main factors affecting the dominant gut bacterial genera.
34498470	6	49	theme	quantitative	1132:1143	arg1	relationships					1166:1178	the quantitative composition-activity relationships	1128:1178	the quantitative composition-activity relationships of polysaccharides in regulating gut microbiota	1128:1226	Our findings reveal that gut microbiota homeostasis could be disordered by HFD at day 2 and provide insights into the quantitative composition-activity relationships of polysaccharides in regulating gut microbiota.
34498470	0	50	theme	Gut	93:95	arg1	Microbiota					97:106	Gut Microbiota	93:106	Gut Microbiota in Short-Term High-Fat Diet-Fed Mice	93:143	Composition-Activity Relationships of Polysaccharides from Saccharina japonica in Regulating Gut Microbiota in Short-Term High-Fat Diet-Fed Mice.
34498470	3	51	theme	12	624:625	arg1	day					627:629	day	627:629	day	627:629	The results showed that a 2 day HFD dramatically altered gut microbiota composition, and the additional 12 day HFD further strengthened the gut microbiota dysbiosis in the HFD group.
34498470	5	52	theme	molecular	916:924	arg1	content					900:906	the sulfate content	888:906	the sulfate content	888:906	Multiple linear regression analysis revealed that the sulfate content and the molecular weight distributions were the main factors affecting the dominant gut bacterial genera.
34498470	5	52	theme	molecular	916:924	arg1	factors					961:967	the main factors	952:967	the main factors affecting the dominant gut bacterial genera	952:1011	Multiple linear regression analysis revealed that the sulfate content and the molecular weight distributions were the main factors affecting the dominant gut bacterial genera.
34498470	5	52	theme	molecular	916:924	arg1	weight					926:931	the molecular weight	912:931	the molecular weight	912:931	Multiple linear regression analysis revealed that the sulfate content and the molecular weight distributions were the main factors affecting the dominant gut bacterial genera.
34498470	6	53	theme	composition-activity	1145:1164	arg1	relationships					1166:1178	the quantitative composition-activity relationships	1128:1178	the quantitative composition-activity relationships of polysaccharides in regulating gut microbiota	1128:1226	Our findings reveal that gut microbiota homeostasis could be disordered by HFD at day 2 and provide insights into the quantitative composition-activity relationships of polysaccharides in regulating gut microbiota.
34498470	3	54	theme	day	627:629	arg1	HFD					631:633	the additional 12 day HFD	609:633	the additional 12 day HFD	609:633	The results showed that a 2 day HFD dramatically altered gut microbiota composition, and the additional 12 day HFD further strengthened the gut microbiota dysbiosis in the HFD group.
34498470	2	55	theme	fractions	417:425	arg1	impact					364:369	the impact	360:369	the impact of eight different S. japonica polysaccharide fractions on the gut microbiota	360:447	In the current study, we investigated the impact of eight different S. japonica polysaccharide fractions on the gut microbiota after day 2 and day 14 treatments on high-fat diet (HFD) feeding mice.
34844507	6	0	theme	porous	907:912	arg1	structure					914:922	an enormous porous structure	895:922	an enormous porous structure in which the size of pores increased by the presence of TTCP particles	895:993	The scaffolds exhibited an enormous porous structure in which the size of pores increased by the presence of TTCP particles.
34844507	1	1	from	solution	428:435	arg1	solution					299:306	a homogenous solution	286:306	a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution	286:435	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	3	2	theme	scratch	668:674	arg1	assay					676:680	scratch assay	668:680	scratch assay for wound healing	668:698	In vitro fibroblast cell viability and migration potential of the scaffolds were determined by MTT, live/dead staining, and scratch assay for wound healing.
34844507	7	3	theme	TTCP	1110:1113	arg1	particles					1115:1123	the initial TTCP particles	1098:1123	the initial TTCP particles	1098:1123	While the polymeric scaffold was amorphous, the formation of low crystalline hydroxyapatite phase and the initial TTCP particles was determined in the composition of TTCP-added scaffolds.
34844507	10	4	theme	TTCP	1359:1362	arg1	concentration					1342:1354	A high concentration	1335:1354	A high concentration of TTCP	1335:1362	A high concentration of TTCP could restrict cell viability, although all the scaffolds were nontoxic.
34844507	1	5	theme	cellulose	324:332	arg1	particles					406:414	hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles	311:414	hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution	311:435	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	8	6	theme	scaffold	1234:1241	arg1	behavior					1208:1215	swelling behavior	1199:1215	swelling behavior of the polymeric scaffold in PBS	1199:1248	TTCP increased swelling behavior of the polymeric scaffold in PBS.
34844507	7	7	theme	phase	1088:1092	arg1	formation					1044:1052	the formation	1040:1052	the formation of low crystalline hydroxyapatite phase and the initial TTCP particles	1040:1123	While the polymeric scaffold was amorphous, the formation of low crystalline hydroxyapatite phase and the initial TTCP particles was determined in the composition of TTCP-added scaffolds.
34844507	0	8	theme	Wound	0:4	arg1	closure					6:12	Wound closure	0:12	Wound closure	0:12	Wound closure, angiogenesis and antibacterial behaviors of tetracalcium phosphate/hydroxyethyl cellulose/hyaluronic acid/gelatin composite dermal scaffolds.
34844507	13	9	theme	TTCP	1737:1740	arg1	presence					1725:1732	The presence	1721:1732	The presence of TTCP	1721:1740	The presence of TTCP had a significant effect on reducing the bacterial activity and 20% TTCP-containing scaffold exhibited better antibacterial activity than the others.
34844507	7	10	theme	crystalline	1061:1071	arg1	phase					1088:1092	low crystalline hydroxyapatite phase	1057:1092	low crystalline hydroxyapatite phase	1057:1092	While the polymeric scaffold was amorphous, the formation of low crystalline hydroxyapatite phase and the initial TTCP particles was determined in the composition of TTCP-added scaffolds.
34844507	3	11	theme	cell	564:567	arg1	viability					569:577	In vitro fibroblast cell viability	544:577	In vitro fibroblast cell viability	544:577	In vitro fibroblast cell viability and migration potential of the scaffolds were determined by MTT, live/dead staining, and scratch assay for wound healing.
34844507	4	12	theme	in	705:706	arg1	test					739:742	The in vivo chick embryo angiogenesis test	701:742	The in vivo chick embryo angiogenesis test	701:742	The in vivo chick embryo angiogenesis test was also carried out.
34844507	11	13	theme	cell	1479:1482	arg1	migration					1484:1492	better cell migration	1472:1492	better cell migration	1472:1492	The scratch assessments determined better cell migration and wound closure in treating with TTCP-containing scaffolds so that after 24 h, a wound closure of 100% was observed.
34844507	13	14	theme	%	1808:1808	arg1	scaffold					1826:1833	20% TTCP-containing scaffold	1806:1833	20% TTCP-containing scaffold	1806:1833	The presence of TTCP had a significant effect on reducing the bacterial activity and 20% TTCP-containing scaffold exhibited better antibacterial activity than the others.
34844507	12	15	theme	embryo	1708:1713	arg1	test					1715:1718	the chick embryo test	1698:1718	the chick embryo test	1698:1718	Furthermore, TTCP-incorporated scaffolds significantly improved the angiogenesis, in the chick embryo test.
34844507	0	16	theme	tetracalcium	59:70	arg1	cellulose/hyaluronic					95:114	tetracalcium phosphate/hydroxyethyl cellulose/hyaluronic	59:114	tetracalcium phosphate/hydroxyethyl cellulose/hyaluronic	59:114	Wound closure, angiogenesis and antibacterial behaviors of tetracalcium phosphate/hydroxyethyl cellulose/hyaluronic acid/gelatin composite dermal scaffolds.
34844507	6	17	theme	particles	985:993	arg1	presence					968:975	the presence	964:975	the presence of TTCP particles	964:993	The scaffolds exhibited an enormous porous structure in which the size of pores increased by the presence of TTCP particles.
34844507	0	18	theme	cellulose/hyaluronic	95:114	arg1	angiogenesis					15:26	angiogenesis	15:26	angiogenesis	15:26	Wound closure, angiogenesis and antibacterial behaviors of tetracalcium phosphate/hydroxyethyl cellulose/hyaluronic acid/gelatin composite dermal scaffolds.
34844507	0	18	theme	cellulose/hyaluronic	95:114	arg1	behaviors					46:54	antibacterial behaviors	32:54	antibacterial behaviors	32:54	Wound closure, angiogenesis and antibacterial behaviors of tetracalcium phosphate/hydroxyethyl cellulose/hyaluronic acid/gelatin composite dermal scaffolds.
34844507	0	18	theme	cellulose/hyaluronic	95:114	arg1	closure					6:12	Wound closure	0:12	Wound closure	0:12	Wound closure, angiogenesis and antibacterial behaviors of tetracalcium phosphate/hydroxyethyl cellulose/hyaluronic acid/gelatin composite dermal scaffolds.
34844507	1	19	theme	-containing	200:210	arg1	scaffolds					222:230	tetracalcium phosphate (TTCP)-containing polymeric scaffolds	171:230	tetracalcium phosphate (TTCP)-containing polymeric scaffolds	171:230	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	1	20	dep	HEC	335:337	arg1	G					370:370	G	370:370	G	370:370	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	1	20	dep	HEC	335:337	arg1	suspension					376:385	suspension	376:385	suspension of 15 or 20% TTCP	376:403	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	1	20	dep	HEC	335:337	arg1	/gelatin					360:367	/hyaluronic acid (HA)/gelatin	339:367	/hyaluronic acid (HA)/gelatin (G)	339:371	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	0	21	theme	composite	129:137	arg1	scaffolds					146:154	composite dermal scaffolds	129:154	composite dermal scaffolds	129:154	Wound closure, angiogenesis and antibacterial behaviors of tetracalcium phosphate/hydroxyethyl cellulose/hyaluronic acid/gelatin composite dermal scaffolds.
34844507	9	22	theme	TTCP	1295:1298	arg1	amount					1285:1290	the amount	1281:1290	the amount of TTCP	1281:1298	The results demonstrated that the amount of TTCP was a crucial factor in cell life.
34844507	9	22	theme	TTCP	1295:1298	arg1	factor					1314:1319	a crucial factor	1304:1319	a crucial factor	1304:1319	The results demonstrated that the amount of TTCP was a crucial factor in cell life.
34844507	9	22	theme	TTCP	1295:1298	arg1	TTCP					1295:1298	TTCP	1295:1298	TTCP	1295:1298	The results demonstrated that the amount of TTCP was a crucial factor in cell life.
34844507	12	23	theme	TTCP-incorporated	1626:1642	arg1	scaffolds					1644:1652	TTCP-incorporated scaffolds	1626:1652	TTCP-incorporated scaffolds	1626:1652	Furthermore, TTCP-incorporated scaffolds significantly improved the angiogenesis, in the chick embryo test.
34844507	13	24	theme	better	1845:1850	arg1	activity					1866:1873	better antibacterial activity	1845:1873	better antibacterial activity	1845:1873	The presence of TTCP had a significant effect on reducing the bacterial activity and 20% TTCP-containing scaffold exhibited better antibacterial activity than the others.
34844507	4	25	theme	chick	713:717	arg1	test					739:742	The in vivo chick embryo angiogenesis test	701:742	The in vivo chick embryo angiogenesis test	701:742	The in vivo chick embryo angiogenesis test was also carried out.
34844507	5	26	theme	initial	779:785	arg1	activity					801:808	the initial antibacterial activity	775:808	the initial antibacterial activity of the scaffolds	775:825	Finally, the initial antibacterial activity of the scaffolds was determined using Staphylococcus aureus.
34844507	4	27	theme	angiogenesis	726:737	arg1	test					739:742	The in vivo chick embryo angiogenesis test	701:742	The in vivo chick embryo angiogenesis test	701:742	The in vivo chick embryo angiogenesis test was also carried out.
34844507	11	28	theme	scratch	1441:1447	arg1	assessments					1449:1459	The scratch assessments	1437:1459	The scratch assessments	1437:1459	The scratch assessments determined better cell migration and wound closure in treating with TTCP-containing scaffolds so that after 24 h, a wound closure of 100% was observed.
34844507	9	29	theme	cell	1324:1327	arg1	life					1329:1332	cell life	1324:1332	cell life	1324:1332	The results demonstrated that the amount of TTCP was a crucial factor in cell life.
34844507	1	30	with	technique	270:278	arg1	solution					299:306	a homogenous solution	286:306	a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution	286:435	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	7	31	theme	TTCP-added	1162:1171	arg1	scaffolds					1173:1181	TTCP-added scaffolds	1162:1181	TTCP-added scaffolds	1162:1181	While the polymeric scaffold was amorphous, the formation of low crystalline hydroxyapatite phase and the initial TTCP particles was determined in the composition of TTCP-added scaffolds.
34844507	1	32	theme	acid	351:354	arg1	G					370:370	G	370:370	G	370:370	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	1	32	theme	acid	351:354	arg1	/gelatin					360:367	/hyaluronic acid (HA)/gelatin	339:367	/hyaluronic acid (HA)/gelatin (G)	339:371	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	2	33	theme	phase	454:458	arg1	composition					460:470	phase composition	454:470	phase composition	454:470	The morphology, phase composition, chemical bands, and swelling behavior of the scaffold were determined.
34844507	11	34	theme	wound	1577:1581	arg1	closure					1583:1589	a wound closure	1575:1589	a wound closure of 100%	1575:1597	The scratch assessments determined better cell migration and wound closure in treating with TTCP-containing scaffolds so that after 24 h, a wound closure of 100% was observed.
34844507	1	35	theme	HA	357:358	arg1	G					370:370	G	370:370	G	370:370	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	1	35	theme	HA	357:358	arg1	/gelatin					360:367	/hyaluronic acid (HA)/gelatin	339:367	/hyaluronic acid (HA)/gelatin (G)	339:371	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	1	36	dep	TTCP	400:403	arg1	%					398:398	%	398:398	%	398:398	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	11	37	theme	%	1597:1597	arg1	closure					1583:1589	a wound closure	1575:1589	a wound closure of 100%	1575:1597	The scratch assessments determined better cell migration and wound closure in treating with TTCP-containing scaffolds so that after 24 h, a wound closure of 100% was observed.
34844507	3	38	theme	wound	686:690	arg1	healing					692:698	wound healing	686:698	wound healing	686:698	In vitro fibroblast cell viability and migration potential of the scaffolds were determined by MTT, live/dead staining, and scratch assay for wound healing.
34844507	7	39	theme	initial	1102:1108	arg1	particles					1115:1123	the initial TTCP particles	1098:1123	the initial TTCP particles	1098:1123	While the polymeric scaffold was amorphous, the formation of low crystalline hydroxyapatite phase and the initial TTCP particles was determined in the composition of TTCP-added scaffolds.
34844507	6	40	theme	enormous	898:905	arg1	structure					914:922	an enormous porous structure	895:922	an enormous porous structure in which the size of pores increased by the presence of TTCP particles	895:993	The scaffolds exhibited an enormous porous structure in which the size of pores increased by the presence of TTCP particles.
34844507	13	41	theme	significant	1748:1758	arg1	effect					1760:1765	a significant effect	1746:1765	a significant effect	1746:1765	The presence of TTCP had a significant effect on reducing the bacterial activity and 20% TTCP-containing scaffold exhibited better antibacterial activity than the others.
34844507	11	42	theme	TTCP-containing	1529:1543	arg1	scaffolds					1545:1553	TTCP-containing scaffolds	1529:1553	TTCP-containing scaffolds so that after 24 h, a wound closure of 100% was observed	1529:1610	The scratch assessments determined better cell migration and wound closure in treating with TTCP-containing scaffolds so that after 24 h, a wound closure of 100% was observed.
34844507	1	43	theme	hydroxyethyl	311:322	arg1	HEC					335:337	HEC	335:337	HEC	335:337	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	1	43	theme	hydroxyethyl	311:322	arg1	cellulose					324:332	hydroxyethyl cellulose	311:332	hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution	311:435	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	7	44	theme	hydroxyapatite	1073:1086	arg1	phase					1088:1092	low crystalline hydroxyapatite phase	1057:1092	low crystalline hydroxyapatite phase	1057:1092	While the polymeric scaffold was amorphous, the formation of low crystalline hydroxyapatite phase and the initial TTCP particles was determined in the composition of TTCP-added scaffolds.
34844507	1	45	theme	polymeric	212:220	arg1	scaffolds					222:230	tetracalcium phosphate (TTCP)-containing polymeric scaffolds	171:230	tetracalcium phosphate (TTCP)-containing polymeric scaffolds	171:230	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	8	46	from	behavior	1208:1215	arg1	PBS					1246:1248	PBS	1246:1248	PBS	1246:1248	TTCP increased swelling behavior of the polymeric scaffold in PBS.
34844507	8	47	theme	polymeric	1224:1232	arg1	scaffold					1234:1241	the polymeric scaffold	1220:1241	the polymeric scaffold	1220:1241	TTCP increased swelling behavior of the polymeric scaffold in PBS.
34844507	1	48	from	particles	406:414	arg1	solution					428:435	HEC/HA/G solution	419:435	HEC/HA/G solution	419:435	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	7	49	theme	low	1057:1059	arg1	phase					1088:1092	low crystalline hydroxyapatite phase	1057:1092	low crystalline hydroxyapatite phase	1057:1092	While the polymeric scaffold was amorphous, the formation of low crystalline hydroxyapatite phase and the initial TTCP particles was determined in the composition of TTCP-added scaffolds.
34844507	3	50	theme	fibroblast	553:562	arg1	viability					569:577	In vitro fibroblast cell viability	544:577	In vitro fibroblast cell viability	544:577	In vitro fibroblast cell viability and migration potential of the scaffolds were determined by MTT, live/dead staining, and scratch assay for wound healing.
34844507	10	51	theme	cell	1379:1382	arg1	viability					1384:1392	cell viability	1379:1392	cell viability	1379:1392	A high concentration of TTCP could restrict cell viability, although all the scaffolds were nontoxic.
34844507	3	52	theme	In	544:545	arg1	viability					569:577	In vitro fibroblast cell viability	544:577	In vitro fibroblast cell viability	544:577	In vitro fibroblast cell viability and migration potential of the scaffolds were determined by MTT, live/dead staining, and scratch assay for wound healing.
34844507	6	53	theme	pores	945:949	arg1	size					937:940	the size	933:940	the size of pores	933:949	The scaffolds exhibited an enormous porous structure in which the size of pores increased by the presence of TTCP particles.
34844507	1	54	from	solution	299:306	arg1	solution					428:435	HEC/HA/G solution	419:435	HEC/HA/G solution	419:435	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	3	55	theme	migration	583:591	arg1	potential					593:601	migration potential	583:601	migration potential	583:601	In vitro fibroblast cell viability and migration potential of the scaffolds were determined by MTT, live/dead staining, and scratch assay for wound healing.
34844507	5	56	theme	scaffolds	817:825	arg1	activity					801:808	the initial antibacterial activity	775:808	the initial antibacterial activity of the scaffolds	775:825	Finally, the initial antibacterial activity of the scaffolds was determined using Staphylococcus aureus.
34844507	8	57	theme	swelling	1199:1206	arg1	behavior					1208:1215	swelling behavior	1199:1215	swelling behavior of the polymeric scaffold in PBS	1199:1248	TTCP increased swelling behavior of the polymeric scaffold in PBS.
34844507	2	58	theme	scaffold	518:525	arg1	behavior					502:509	swelling behavior	493:509	swelling behavior	493:509	The morphology, phase composition, chemical bands, and swelling behavior of the scaffold were determined.
34844507	2	58	theme	scaffold	518:525	arg1	morphology					442:451	The morphology	438:451	The morphology	438:451	The morphology, phase composition, chemical bands, and swelling behavior of the scaffold were determined.
34844507	2	58	theme	scaffold	518:525	arg1	composition					460:470	phase composition	454:470	phase composition	454:470	The morphology, phase composition, chemical bands, and swelling behavior of the scaffold were determined.
34844507	2	58	theme	scaffold	518:525	arg1	bands					482:486	chemical bands	473:486	chemical bands	473:486	The morphology, phase composition, chemical bands, and swelling behavior of the scaffold were determined.
34844507	1	59	theme	TTCP	400:403	arg1	G					370:370	G	370:370	G	370:370	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	1	59	theme	TTCP	400:403	arg1	suspension					376:385	suspension	376:385	suspension of 15 or 20% TTCP	376:403	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	1	59	theme	TTCP	400:403	arg1	/gelatin					360:367	/hyaluronic acid (HA)/gelatin	339:367	/hyaluronic acid (HA)/gelatin (G)	339:371	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	11	60	theme	better	1472:1477	arg1	migration					1484:1492	better cell migration	1472:1492	better cell migration	1472:1492	The scratch assessments determined better cell migration and wound closure in treating with TTCP-containing scaffolds so that after 24 h, a wound closure of 100% was observed.
34844507	7	61	theme	polymeric	1006:1014	arg1	amorphous					1029:1037	amorphous	1029:1037	amorphous	1029:1037	While the polymeric scaffold was amorphous, the formation of low crystalline hydroxyapatite phase and the initial TTCP particles was determined in the composition of TTCP-added scaffolds.
34844507	7	61	theme	polymeric	1006:1014	arg1	scaffold					1016:1023	the polymeric scaffold	1002:1023	the polymeric scaffold	1002:1023	While the polymeric scaffold was amorphous, the formation of low crystalline hydroxyapatite phase and the initial TTCP particles was determined in the composition of TTCP-added scaffolds.
34844507	1	62	theme	particles	406:414	arg1	solution					299:306	a homogenous solution	286:306	a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution	286:435	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	3	63	theme	scaffolds	610:618	arg1	viability					569:577	In vitro fibroblast cell viability	544:577	In vitro fibroblast cell viability	544:577	In vitro fibroblast cell viability and migration potential of the scaffolds were determined by MTT, live/dead staining, and scratch assay for wound healing.
34844507	3	63	theme	scaffolds	610:618	arg1	potential					593:601	migration potential	583:601	migration potential	583:601	In vitro fibroblast cell viability and migration potential of the scaffolds were determined by MTT, live/dead staining, and scratch assay for wound healing.
34844507	0	64	theme	phosphate/hydroxyethyl	72:93	arg1	cellulose/hyaluronic					95:114	tetracalcium phosphate/hydroxyethyl cellulose/hyaluronic	59:114	tetracalcium phosphate/hydroxyethyl cellulose/hyaluronic	59:114	Wound closure, angiogenesis and antibacterial behaviors of tetracalcium phosphate/hydroxyethyl cellulose/hyaluronic acid/gelatin composite dermal scaffolds.
34844507	1	65	theme	HEC/HA/G	419:426	arg1	solution					428:435	HEC/HA/G solution	419:435	HEC/HA/G solution	419:435	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	3	66	dep	In	544:545	arg1	vitro					547:551	vitro	547:551	vitro	547:551	In vitro fibroblast cell viability and migration potential of the scaffolds were determined by MTT, live/dead staining, and scratch assay for wound healing.
34844507	12	67	theme	chick	1702:1706	arg1	test					1715:1718	the chick embryo test	1698:1718	the chick embryo test	1698:1718	Furthermore, TTCP-incorporated scaffolds significantly improved the angiogenesis, in the chick embryo test.
34844507	11	68	theme	wound	1498:1502	arg1	closure					1504:1510	wound closure	1498:1510	wound closure	1498:1510	The scratch assessments determined better cell migration and wound closure in treating with TTCP-containing scaffolds so that after 24 h, a wound closure of 100% was observed.
34844507	6	69	theme	TTCP	980:983	arg1	particles					985:993	TTCP particles	980:993	TTCP particles	980:993	The scaffolds exhibited an enormous porous structure in which the size of pores increased by the presence of TTCP particles.
34844507	2	70	theme	chemical	473:480	arg1	bands					482:486	chemical bands	473:486	chemical bands	473:486	The morphology, phase composition, chemical bands, and swelling behavior of the scaffold were determined.
34844507	0	71	theme	dermal	139:144	arg1	scaffolds					146:154	composite dermal scaffolds	129:154	composite dermal scaffolds	129:154	Wound closure, angiogenesis and antibacterial behaviors of tetracalcium phosphate/hydroxyethyl cellulose/hyaluronic acid/gelatin composite dermal scaffolds.
34844507	13	72	contain	had	1742:1744	arg1	presence					1725:1732	The presence	1721:1732	The presence of TTCP	1721:1740	The presence of TTCP had a significant effect on reducing the bacterial activity and 20% TTCP-containing scaffold exhibited better antibacterial activity than the others.
34844507	13	72	contain	had	1742:1744	arg2	effect					1760:1765	a significant effect	1746:1765	a significant effect	1746:1765	The presence of TTCP had a significant effect on reducing the bacterial activity and 20% TTCP-containing scaffold exhibited better antibacterial activity than the others.
34844507	7	73	theme	scaffolds	1173:1181	arg1	composition					1147:1157	the composition	1143:1157	the composition of TTCP-added scaffolds	1143:1181	While the polymeric scaffold was amorphous, the formation of low crystalline hydroxyapatite phase and the initial TTCP particles was determined in the composition of TTCP-added scaffolds.
34844507	2	74	theme	swelling	493:500	arg1	behavior					502:509	swelling behavior	493:509	swelling behavior	493:509	The morphology, phase composition, chemical bands, and swelling behavior of the scaffold were determined.
34844507	13	75	theme	antibacterial	1852:1864	arg1	activity					1866:1873	better antibacterial activity	1845:1873	better antibacterial activity	1845:1873	The presence of TTCP had a significant effect on reducing the bacterial activity and 20% TTCP-containing scaffold exhibited better antibacterial activity than the others.
34844507	9	76	theme	crucial	1306:1312	arg1	amount					1285:1290	the amount	1281:1290	the amount of TTCP	1281:1298	The results demonstrated that the amount of TTCP was a crucial factor in cell life.
34844507	9	76	theme	crucial	1306:1312	arg1	factor					1314:1319	a crucial factor	1304:1319	a crucial factor	1304:1319	The results demonstrated that the amount of TTCP was a crucial factor in cell life.
34844507	9	76	theme	crucial	1306:1312	arg1	TTCP					1295:1298	TTCP	1295:1298	TTCP	1295:1298	The results demonstrated that the amount of TTCP was a crucial factor in cell life.
34844507	9	77	from	factor	1314:1319	arg1	life					1329:1332	cell life	1324:1332	cell life	1324:1332	The results demonstrated that the amount of TTCP was a crucial factor in cell life.
34844507	4	78	dep	in	705:706	arg1	vivo					708:711	vivo	708:711	vivo	708:711	The in vivo chick embryo angiogenesis test was also carried out.
34844507	13	79	theme	20	1806:1807	arg1	%					1808:1808	%	1808:1808	%	1808:1808	The presence of TTCP had a significant effect on reducing the bacterial activity and 20% TTCP-containing scaffold exhibited better antibacterial activity than the others.
34844507	5	80	theme	antibacterial	787:799	arg1	activity					801:808	the initial antibacterial activity	775:808	the initial antibacterial activity of the scaffolds	775:825	Finally, the initial antibacterial activity of the scaffolds was determined using Staphylococcus aureus.
34844507	10	81	theme	high	1337:1340	arg1	concentration					1342:1354	A high concentration	1335:1354	A high concentration of TTCP	1335:1362	A high concentration of TTCP could restrict cell viability, although all the scaffolds were nontoxic.
34844507	4	82	theme	embryo	719:724	arg1	test					739:742	The in vivo chick embryo angiogenesis test	701:742	The in vivo chick embryo angiogenesis test	701:742	The in vivo chick embryo angiogenesis test was also carried out.
34844507	13	83	theme	TTCP-containing	1810:1824	arg1	scaffold					1826:1833	20% TTCP-containing scaffold	1806:1833	20% TTCP-containing scaffold	1806:1833	The presence of TTCP had a significant effect on reducing the bacterial activity and 20% TTCP-containing scaffold exhibited better antibacterial activity than the others.
34844507	0	84	theme	antibacterial	32:44	arg1	behaviors					46:54	antibacterial behaviors	32:54	antibacterial behaviors	32:54	Wound closure, angiogenesis and antibacterial behaviors of tetracalcium phosphate/hydroxyethyl cellulose/hyaluronic acid/gelatin composite dermal scaffolds.
34844507	1	85	theme	/hyaluronic	339:349	arg1	G					370:370	G	370:370	G	370:370	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	1	85	theme	/hyaluronic	339:349	arg1	/gelatin					360:367	/hyaluronic acid (HA)/gelatin	339:367	/hyaluronic acid (HA)/gelatin (G)	339:371	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	1	86	theme	freeze-drying	256:268	arg1	technique					270:278	a freeze-drying technique	254:278	a freeze-drying technique	254:278	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	7	87	theme	particles	1115:1123	arg1	formation					1044:1052	the formation	1040:1052	the formation of low crystalline hydroxyapatite phase and the initial TTCP particles	1040:1123	While the polymeric scaffold was amorphous, the formation of low crystalline hydroxyapatite phase and the initial TTCP particles was determined in the composition of TTCP-added scaffolds.
34844507	1	88	dep	%	398:398	arg1	20					396:397	20	396:397	20	396:397	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	1	88	dep	%	398:398	arg1	15					390:391	15	390:391	15	390:391	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
34844507	3	89	theme	live/dead	644:652	arg1	staining					654:661	live/dead staining	644:661	live/dead staining	644:661	In vitro fibroblast cell viability and migration potential of the scaffolds were determined by MTT, live/dead staining, and scratch assay for wound healing.
34844507	13	90	theme	bacterial	1783:1791	arg1	activity					1793:1800	the bacterial activity	1779:1800	the bacterial activity	1779:1800	The presence of TTCP had a significant effect on reducing the bacterial activity and 20% TTCP-containing scaffold exhibited better antibacterial activity than the others.
34844507	1	91	theme	homogenous	288:297	arg1	solution					299:306	a homogenous solution	286:306	a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution	286:435	Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution.
33010593	6	0	theme	genes	1057:1061	arg1	level					1039:1043	the expression level	1024:1043	the expression level of some key genes in hepatic lipid metabolism	1024:1089	Meanwhile, the expression level of some key genes in hepatic lipid metabolism dysregulated significantly in the thiram-exposed chickens.
33010593	6	0	theme	genes	1057:1061	arg1	Meanwhile					1013:1021	Meanwhile	1013:1021	Meanwhile	1013:1021	Meanwhile, the expression level of some key genes in hepatic lipid metabolism dysregulated significantly in the thiram-exposed chickens.
33010593	2	1	from	effects	296:302	arg1	metabolism					323:332	lipid metabolism	317:332	lipid metabolism by perturbing gut microbiota of chickens	317:373	However, the effects of thiram on lipid metabolism by perturbing gut microbiota of chickens are not clear.
33010593	0	2	theme	metabolism	72:81	arg1	disorder					83:90	lipid metabolism disorder	66:90	lipid metabolism disorder in chickens	66:102	The fungicide thiram perturbs gut microbiota community and causes lipid metabolism disorder in chickens.
33010593	6	3	from	level	1039:1043	arg1	metabolism					1080:1089	hepatic lipid metabolism	1066:1089	hepatic lipid metabolism	1066:1089	Meanwhile, the expression level of some key genes in hepatic lipid metabolism dysregulated significantly in the thiram-exposed chickens.
33010593	2	4	theme	gut	348:350	arg1	microbiota					352:361	gut microbiota	348:361	gut microbiota of chickens	348:373	However, the effects of thiram on lipid metabolism by perturbing gut microbiota of chickens are not clear.
33010593	1	5	theme	various	252:258	arg1	products					273:280	various agricultural products	252:280	various agricultural products	252:280	Fungicide thiram, a representative dithiocarbamate pesticide can cause potential health hazards to humans and animal health due to the residues in various agricultural products.
33010593	9	6	theme	Correlation	1618:1628	arg1	analysis					1630:1637	Correlation analysis	1618:1637	Correlation analysis	1618:1637	Correlation analysis further revealed a significant association between microorganisms and lipid metabolism-related parameters.
33010593	9	7	theme	significant	1658:1668	arg1	association					1670:1680	a significant association	1656:1680	a significant association between microorganisms and lipid metabolism-related parameters	1656:1743	Correlation analysis further revealed a significant association between microorganisms and lipid metabolism-related parameters.
33010593	3	8	theme	acute	498:502	arg1	chickens					519:526	acute thiram-exposed chickens	498:526	acute thiram-exposed chickens	498:526	Our study was aimed to explore the protective of polysaccharide extracted from Morinda officinalis (MOP) on acute thiram-exposed chickens, and to analyze the association between alteration of gut microbiota and lipid metabolism.
33010593	5	9	theme	thiram	835:840	arg1	exposure					842:849	thiram exposure	835:849	thiram exposure	835:849	The results showed that thiram exposure prominently elevated liver index, changed liver function by histopathological examination and serum biochemistry diagnoses, and increased blood lipid parameters.
33010593	4	10	theme	thiram-treated	707:720	arg1	diet					722:725	a thiram-treated diet	705:725	a thiram-treated diet supplemented with 250, 500, or 1000 mg/kg MOP was used in this study, respectively	705:808	Three hundred chicks are fed with a normal diet, thiram-treated diet (100 mg/kg), and a thiram-treated diet supplemented with 250, 500, or 1000 mg/kg MOP was used in this study, respectively.
33010593	1	11	theme	agricultural	260:271	arg1	products					273:280	various agricultural products	252:280	various agricultural products	252:280	Fungicide thiram, a representative dithiocarbamate pesticide can cause potential health hazards to humans and animal health due to the residues in various agricultural products.
33010593	6	12	theme	thiram-exposed	1125:1138	arg1	chickens					1140:1147	the thiram-exposed chickens	1121:1147	the thiram-exposed chickens	1121:1147	Meanwhile, the expression level of some key genes in hepatic lipid metabolism dysregulated significantly in the thiram-exposed chickens.
33010593	11	13	theme	thiram	1867:1872	arg1	exposure					1874:1881	thiram exposure	1867:1881	thiram exposure	1867:1881	Together, these data suggest that thiram exposure causes the imbalance of the gut microbiota and hepatic lipid metabolism disorder in chickens.
33010593	11	14	theme	microbiota	1915:1924	arg1	disorder					1955:1962	the gut microbiota and hepatic lipid metabolism disorder	1907:1962	the gut microbiota and hepatic lipid metabolism disorder	1907:1962	Together, these data suggest that thiram exposure causes the imbalance of the gut microbiota and hepatic lipid metabolism disorder in chickens.
33010593	10	15	theme	500 mg/kg	1762:1770	arg1	MOP					1772:1774	500 mg/kg MOP	1762:1774	500 mg/kg MOP	1762:1774	Optimistically, 500 mg/kg MOP can alleviate the damage of thiram in the gut and liver.
33010593	4	16	used	used	777:780	arg2	MOP					769:771	250, 500, or 1000 mg/kg MOP	745:771	250, 500, or 1000 mg/kg MOP	745:771	Three hundred chicks are fed with a normal diet, thiram-treated diet (100 mg/kg), and a thiram-treated diet supplemented with 250, 500, or 1000 mg/kg MOP was used in this study, respectively.
33010593	1	17	theme	representative	125:138	arg1	pesticide					156:164	a representative dithiocarbamate pesticide	123:164	a representative dithiocarbamate pesticide	123:164	Fungicide thiram, a representative dithiocarbamate pesticide can cause potential health hazards to humans and animal health due to the residues in various agricultural products.
33010593	1	17	theme	representative	125:138	arg1	thiram					115:120	Fungicide thiram	105:120	Fungicide thiram	105:120	Fungicide thiram, a representative dithiocarbamate pesticide can cause potential health hazards to humans and animal health due to the residues in various agricultural products.
33010593	4	18	theme	thiram-treated	668:681	arg1	100 mg/kg					689:697	100 mg/kg	689:697	100 mg/kg	689:697	Three hundred chicks are fed with a normal diet, thiram-treated diet (100 mg/kg), and a thiram-treated diet supplemented with 250, 500, or 1000 mg/kg MOP was used in this study, respectively.
33010593	4	18	theme	thiram-treated	668:681	arg1	diet					683:686	thiram-treated diet	668:686	thiram-treated diet (100 mg/kg)	668:698	Three hundred chicks are fed with a normal diet, thiram-treated diet (100 mg/kg), and a thiram-treated diet supplemented with 250, 500, or 1000 mg/kg MOP was used in this study, respectively.
33010593	7	19	theme	broiler	1291:1297	arg1	microbiota					1305:1314	the broiler fecal microbiota	1287:1314	the broiler fecal microbiota	1287:1314	Furthermore, 16S rRNA gene sequencing indicated that thiram exposure can significantly alter the richness, diversity, and composition of the broiler fecal microbiota, and the relative abundance of Firmicutes and Proteobacteria was also affected at the phylum level.
33010593	7	20	theme	phylum	1402:1407	arg1	level					1409:1413	the phylum level	1398:1413	the phylum level	1398:1413	Furthermore, 16S rRNA gene sequencing indicated that thiram exposure can significantly alter the richness, diversity, and composition of the broiler fecal microbiota, and the relative abundance of Firmicutes and Proteobacteria was also affected at the phylum level.
33010593	7	21	theme	fecal	1299:1303	arg1	microbiota					1305:1314	the broiler fecal microbiota	1287:1314	the broiler fecal microbiota	1287:1314	Furthermore, 16S rRNA gene sequencing indicated that thiram exposure can significantly alter the richness, diversity, and composition of the broiler fecal microbiota, and the relative abundance of Firmicutes and Proteobacteria was also affected at the phylum level.
33010593	1	22	theme	potential	176:184	arg1	hazards					193:199	potential health hazards	176:199	potential health hazards	176:199	Fungicide thiram, a representative dithiocarbamate pesticide can cause potential health hazards to humans and animal health due to the residues in various agricultural products.
33010593	7	23	theme	gene	1172:1175	arg1	sequencing					1177:1186	16S rRNA gene sequencing	1163:1186	16S rRNA gene sequencing	1163:1186	Furthermore, 16S rRNA gene sequencing indicated that thiram exposure can significantly alter the richness, diversity, and composition of the broiler fecal microbiota, and the relative abundance of Firmicutes and Proteobacteria was also affected at the phylum level.
33010593	5	24	theme	serum	945:949	arg1	diagnoses					964:972	serum biochemistry diagnoses	945:972	serum biochemistry diagnoses	945:972	The results showed that thiram exposure prominently elevated liver index, changed liver function by histopathological examination and serum biochemistry diagnoses, and increased blood lipid parameters.
33010593	6	25	theme	key	1053:1055	arg1	genes					1057:1061	some key genes	1048:1061	some key genes	1048:1061	Meanwhile, the expression level of some key genes in hepatic lipid metabolism dysregulated significantly in the thiram-exposed chickens.
33010593	1	26	theme	health	186:191	arg1	hazards					193:199	potential health hazards	176:199	potential health hazards	176:199	Fungicide thiram, a representative dithiocarbamate pesticide can cause potential health hazards to humans and animal health due to the residues in various agricultural products.
33010593	0	27	theme	fungicide	4:12	arg1	thiram					14:19	The fungicide thiram	0:19	The fungicide thiram	0:19	The fungicide thiram perturbs gut microbiota community and causes lipid metabolism disorder in chickens.
33010593	7	28	theme	thiram	1203:1208	arg1	exposure					1210:1217	thiram exposure	1203:1217	thiram exposure	1203:1217	Furthermore, 16S rRNA gene sequencing indicated that thiram exposure can significantly alter the richness, diversity, and composition of the broiler fecal microbiota, and the relative abundance of Firmicutes and Proteobacteria was also affected at the phylum level.
33010593	3	29	from	officinalis	477:487	arg1	chickens					519:526	acute thiram-exposed chickens	498:526	acute thiram-exposed chickens	498:526	Our study was aimed to explore the protective of polysaccharide extracted from Morinda officinalis (MOP) on acute thiram-exposed chickens, and to analyze the association between alteration of gut microbiota and lipid metabolism.
33010593	11	30	theme	metabolism	1944:1953	arg1	disorder					1955:1962	the gut microbiota and hepatic lipid metabolism disorder	1907:1962	the gut microbiota and hepatic lipid metabolism disorder	1907:1962	Together, these data suggest that thiram exposure causes the imbalance of the gut microbiota and hepatic lipid metabolism disorder in chickens.
33010593	11	31	theme	lipid	1938:1942	arg1	metabolism					1944:1953	hepatic lipid metabolism	1930:1953	hepatic lipid metabolism	1930:1953	Together, these data suggest that thiram exposure causes the imbalance of the gut microbiota and hepatic lipid metabolism disorder in chickens.
33010593	4	32	theme	normal	655:660	arg1	diet					662:665	a normal diet	653:665	a normal diet	653:665	Three hundred chicks are fed with a normal diet, thiram-treated diet (100 mg/kg), and a thiram-treated diet supplemented with 250, 500, or 1000 mg/kg MOP was used in this study, respectively.
33010593	7	33	theme	16S	1163:1165	arg1	sequencing					1177:1186	16S rRNA gene sequencing	1163:1186	16S rRNA gene sequencing	1163:1186	Furthermore, 16S rRNA gene sequencing indicated that thiram exposure can significantly alter the richness, diversity, and composition of the broiler fecal microbiota, and the relative abundance of Firmicutes and Proteobacteria was also affected at the phylum level.
33010593	9	34	theme	lipid	1709:1713	arg1	parameters					1734:1743	lipid metabolism-related parameters	1709:1743	lipid metabolism-related parameters	1709:1743	Correlation analysis further revealed a significant association between microorganisms and lipid metabolism-related parameters.
33010593	11	35	theme	hepatic	1930:1936	arg1	metabolism					1944:1953	hepatic lipid metabolism	1930:1953	hepatic lipid metabolism	1930:1953	Together, these data suggest that thiram exposure causes the imbalance of the gut microbiota and hepatic lipid metabolism disorder in chickens.
33010593	2	36	theme	thiram	307:312	arg1	clear					383:387	clear	383:387	clear	383:387	However, the effects of thiram on lipid metabolism by perturbing gut microbiota of chickens are not clear.
33010593	2	36	theme	thiram	307:312	arg1	effects					296:302	the effects	292:302	the effects of thiram on lipid metabolism by perturbing gut microbiota of chickens	292:373	However, the effects of thiram on lipid metabolism by perturbing gut microbiota of chickens are not clear.
33010593	6	37	theme	expression	1028:1037	arg1	level					1039:1043	the expression level	1024:1043	the expression level of some key genes in hepatic lipid metabolism	1024:1089	Meanwhile, the expression level of some key genes in hepatic lipid metabolism dysregulated significantly in the thiram-exposed chickens.
33010593	6	37	theme	expression	1028:1037	arg1	Meanwhile					1013:1021	Meanwhile	1013:1021	Meanwhile	1013:1021	Meanwhile, the expression level of some key genes in hepatic lipid metabolism dysregulated significantly in the thiram-exposed chickens.
33010593	7	38	theme	Firmicutes	1347:1356	arg1	abundance					1334:1342	the relative abundance	1321:1342	the relative abundance of Firmicutes and Proteobacteria	1321:1375	Furthermore, 16S rRNA gene sequencing indicated that thiram exposure can significantly alter the richness, diversity, and composition of the broiler fecal microbiota, and the relative abundance of Firmicutes and Proteobacteria was also affected at the phylum level.
33010593	9	39	theme	metabolism-related	1715:1732	arg1	parameters					1734:1743	lipid metabolism-related parameters	1709:1743	lipid metabolism-related parameters	1709:1743	Correlation analysis further revealed a significant association between microorganisms and lipid metabolism-related parameters.
33010593	7	40	theme	Proteobacteria	1362:1375	arg1	abundance					1334:1342	the relative abundance	1321:1342	the relative abundance of Firmicutes and Proteobacteria	1321:1375	Furthermore, 16S rRNA gene sequencing indicated that thiram exposure can significantly alter the richness, diversity, and composition of the broiler fecal microbiota, and the relative abundance of Firmicutes and Proteobacteria was also affected at the phylum level.
33010593	11	41	theme	disorder	1955:1962	arg1	imbalance					1894:1902	the imbalance	1890:1902	the imbalance of the gut microbiota and hepatic lipid metabolism disorder in chickens	1890:1974	Together, these data suggest that thiram exposure causes the imbalance of the gut microbiota and hepatic lipid metabolism disorder in chickens.
33010593	5	42	theme	liver	893:897	arg1	function					899:906	liver function	893:906	liver function	893:906	The results showed that thiram exposure prominently elevated liver index, changed liver function by histopathological examination and serum biochemistry diagnoses, and increased blood lipid parameters.
33010593	0	43	from	disorder	83:90	arg1	chickens					95:102	chickens	95:102	chickens	95:102	The fungicide thiram perturbs gut microbiota community and causes lipid metabolism disorder in chickens.
33010593	1	44	theme	Fungicide	105:113	arg1	pesticide					156:164	a representative dithiocarbamate pesticide	123:164	a representative dithiocarbamate pesticide	123:164	Fungicide thiram, a representative dithiocarbamate pesticide can cause potential health hazards to humans and animal health due to the residues in various agricultural products.
33010593	1	44	theme	Fungicide	105:113	arg1	thiram					115:120	Fungicide thiram	105:120	Fungicide thiram	105:120	Fungicide thiram, a representative dithiocarbamate pesticide can cause potential health hazards to humans and animal health due to the residues in various agricultural products.
33010593	1	45	theme	dithiocarbamate	140:154	arg1	pesticide					156:164	a representative dithiocarbamate pesticide	123:164	a representative dithiocarbamate pesticide	123:164	Fungicide thiram, a representative dithiocarbamate pesticide can cause potential health hazards to humans and animal health due to the residues in various agricultural products.
33010593	1	45	theme	dithiocarbamate	140:154	arg1	thiram					115:120	Fungicide thiram	105:120	Fungicide thiram	105:120	Fungicide thiram, a representative dithiocarbamate pesticide can cause potential health hazards to humans and animal health due to the residues in various agricultural products.
33010593	0	46	theme	microbiota	34:43	arg1	community					45:53	gut microbiota community	30:53	gut microbiota community	30:53	The fungicide thiram perturbs gut microbiota community and causes lipid metabolism disorder in chickens.
33010593	3	47	theme	of	436:437	arg1	polysaccharide					439:452	the protective of polysaccharide	421:452	the protective of polysaccharide extracted from Morinda officinalis (MOP) on acute thiram-exposed chickens	421:526	Our study was aimed to explore the protective of polysaccharide extracted from Morinda officinalis (MOP) on acute thiram-exposed chickens, and to analyze the association between alteration of gut microbiota and lipid metabolism.
33010593	3	48	theme	gut	582:584	arg1	microbiota					586:595	gut microbiota	582:595	gut microbiota	582:595	Our study was aimed to explore the protective of polysaccharide extracted from Morinda officinalis (MOP) on acute thiram-exposed chickens, and to analyze the association between alteration of gut microbiota and lipid metabolism.
33010593	7	49	theme	microbiota	1305:1314	arg1	diversity					1257:1265	diversity	1257:1265	diversity	1257:1265	Furthermore, 16S rRNA gene sequencing indicated that thiram exposure can significantly alter the richness, diversity, and composition of the broiler fecal microbiota, and the relative abundance of Firmicutes and Proteobacteria was also affected at the phylum level.
33010593	7	49	theme	microbiota	1305:1314	arg1	composition					1272:1282	composition	1272:1282	composition	1272:1282	Furthermore, 16S rRNA gene sequencing indicated that thiram exposure can significantly alter the richness, diversity, and composition of the broiler fecal microbiota, and the relative abundance of Firmicutes and Proteobacteria was also affected at the phylum level.
33010593	7	49	theme	microbiota	1305:1314	arg1	richness					1247:1254	richness	1247:1254	richness	1247:1254	Furthermore, 16S rRNA gene sequencing indicated that thiram exposure can significantly alter the richness, diversity, and composition of the broiler fecal microbiota, and the relative abundance of Firmicutes and Proteobacteria was also affected at the phylum level.
33010593	1	50	theme	animal	215:220	arg1	health					222:227	animal health	215:227	animal health	215:227	Fungicide thiram, a representative dithiocarbamate pesticide can cause potential health hazards to humans and animal health due to the residues in various agricultural products.
33010593	0	51	theme	gut	30:32	arg1	community					45:53	gut microbiota community	30:53	gut microbiota community	30:53	The fungicide thiram perturbs gut microbiota community and causes lipid metabolism disorder in chickens.
33010593	8	52	theme	microbial	1434:1442	arg1	Butyricicoccus					1522:1535	Butyricicoccus	1522:1535	Butyricicoccus	1522:1535	In addition, some microbial populations including Lactobacillus, Ruminococcus, Oscillospira, Blautia, and Butyricicoccus significantly decreased at the genus level, whereas the Klebsiella was opposite.
33010593	8	52	theme	microbial	1434:1442	arg1	Ruminococcus					1481:1492	Ruminococcus	1481:1492	Ruminococcus	1481:1492	In addition, some microbial populations including Lactobacillus, Ruminococcus, Oscillospira, Blautia, and Butyricicoccus significantly decreased at the genus level, whereas the Klebsiella was opposite.
33010593	8	52	theme	microbial	1434:1442	arg1	Oscillospira					1495:1506	Oscillospira	1495:1506	Oscillospira	1495:1506	In addition, some microbial populations including Lactobacillus, Ruminococcus, Oscillospira, Blautia, and Butyricicoccus significantly decreased at the genus level, whereas the Klebsiella was opposite.
33010593	8	52	theme	microbial	1434:1442	arg1	populations					1444:1454	some microbial populations	1429:1454	some microbial populations including Lactobacillus, Ruminococcus, Oscillospira, Blautia, and Butyricicoccus	1429:1535	In addition, some microbial populations including Lactobacillus, Ruminococcus, Oscillospira, Blautia, and Butyricicoccus significantly decreased at the genus level, whereas the Klebsiella was opposite.
33010593	8	52	theme	microbial	1434:1442	arg1	Lactobacillus					1466:1478	Lactobacillus	1466:1478	Lactobacillus	1466:1478	In addition, some microbial populations including Lactobacillus, Ruminococcus, Oscillospira, Blautia, and Butyricicoccus significantly decreased at the genus level, whereas the Klebsiella was opposite.
33010593	8	52	theme	microbial	1434:1442	arg1	Blautia					1509:1515	Blautia	1509:1515	Blautia	1509:1515	In addition, some microbial populations including Lactobacillus, Ruminococcus, Oscillospira, Blautia, and Butyricicoccus significantly decreased at the genus level, whereas the Klebsiella was opposite.
33010593	3	53	theme	microbiota	586:595	arg1	alteration					568:577	alteration	568:577	alteration of gut microbiota and lipid metabolism	568:616	Our study was aimed to explore the protective of polysaccharide extracted from Morinda officinalis (MOP) on acute thiram-exposed chickens, and to analyze the association between alteration of gut microbiota and lipid metabolism.
33010593	10	54	from	damage	1794:1799	arg1	liver					1826:1830	liver	1826:1830	liver	1826:1830	Optimistically, 500 mg/kg MOP can alleviate the damage of thiram in the gut and liver.
33010593	10	54	from	damage	1794:1799	arg1	gut					1818:1820	gut	1818:1820	gut	1818:1820	Optimistically, 500 mg/kg MOP can alleviate the damage of thiram in the gut and liver.
33010593	8	55	theme	genus	1568:1572	arg1	level					1574:1578	the genus level	1564:1578	the genus level	1564:1578	In addition, some microbial populations including Lactobacillus, Ruminococcus, Oscillospira, Blautia, and Butyricicoccus significantly decreased at the genus level, whereas the Klebsiella was opposite.
33010593	5	56	theme	lipid	995:999	arg1	parameters					1001:1010	blood lipid parameters	989:1010	blood lipid parameters	989:1010	The results showed that thiram exposure prominently elevated liver index, changed liver function by histopathological examination and serum biochemistry diagnoses, and increased blood lipid parameters.
33010593	6	57	theme	hepatic	1066:1072	arg1	metabolism					1080:1089	hepatic lipid metabolism	1066:1089	hepatic lipid metabolism	1066:1089	Meanwhile, the expression level of some key genes in hepatic lipid metabolism dysregulated significantly in the thiram-exposed chickens.
33010593	11	58	from	imbalance	1894:1902	arg1	chickens					1967:1974	chickens	1967:1974	chickens	1967:1974	Together, these data suggest that thiram exposure causes the imbalance of the gut microbiota and hepatic lipid metabolism disorder in chickens.
33010593	1	59	from	residues	240:247	arg1	products					273:280	various agricultural products	252:280	various agricultural products	252:280	Fungicide thiram, a representative dithiocarbamate pesticide can cause potential health hazards to humans and animal health due to the residues in various agricultural products.
33010593	11	60	theme	gut	1911:1913	arg1	microbiota					1915:1924	gut microbiota	1911:1924	gut microbiota	1911:1924	Together, these data suggest that thiram exposure causes the imbalance of the gut microbiota and hepatic lipid metabolism disorder in chickens.
33010593	5	61	theme	liver	872:876	arg1	index					878:882	liver index	872:882	liver index	872:882	The results showed that thiram exposure prominently elevated liver index, changed liver function by histopathological examination and serum biochemistry diagnoses, and increased blood lipid parameters.
33010593	10	62	theme	thiram	1804:1809	arg1	damage					1794:1799	the damage	1790:1799	the damage of thiram in the gut and liver	1790:1830	Optimistically, 500 mg/kg MOP can alleviate the damage of thiram in the gut and liver.
33010593	7	63	theme	relative	1325:1332	arg1	abundance					1334:1342	the relative abundance	1321:1342	the relative abundance of Firmicutes and Proteobacteria	1321:1375	Furthermore, 16S rRNA gene sequencing indicated that thiram exposure can significantly alter the richness, diversity, and composition of the broiler fecal microbiota, and the relative abundance of Firmicutes and Proteobacteria was also affected at the phylum level.
33010593	5	64	theme	biochemistry	951:962	arg1	diagnoses					964:972	serum biochemistry diagnoses	945:972	serum biochemistry diagnoses	945:972	The results showed that thiram exposure prominently elevated liver index, changed liver function by histopathological examination and serum biochemistry diagnoses, and increased blood lipid parameters.
33010593	3	65	theme	metabolism	607:616	arg1	alteration					568:577	alteration	568:577	alteration of gut microbiota and lipid metabolism	568:616	Our study was aimed to explore the protective of polysaccharide extracted from Morinda officinalis (MOP) on acute thiram-exposed chickens, and to analyze the association between alteration of gut microbiota and lipid metabolism.
33010593	5	66	theme	blood	989:993	arg1	parameters					1001:1010	blood lipid parameters	989:1010	blood lipid parameters	989:1010	The results showed that thiram exposure prominently elevated liver index, changed liver function by histopathological examination and serum biochemistry diagnoses, and increased blood lipid parameters.
33010593	3	67	theme	Morinda	469:475	arg1	MOP					490:492	MOP	490:492	MOP	490:492	Our study was aimed to explore the protective of polysaccharide extracted from Morinda officinalis (MOP) on acute thiram-exposed chickens, and to analyze the association between alteration of gut microbiota and lipid metabolism.
33010593	3	67	theme	Morinda	469:475	arg1	officinalis					477:487	Morinda officinalis	469:487	Morinda officinalis (MOP) on acute thiram-exposed chickens	469:526	Our study was aimed to explore the protective of polysaccharide extracted from Morinda officinalis (MOP) on acute thiram-exposed chickens, and to analyze the association between alteration of gut microbiota and lipid metabolism.
33010593	6	68	theme	lipid	1074:1078	arg1	metabolism					1080:1089	hepatic lipid metabolism	1066:1089	hepatic lipid metabolism	1066:1089	Meanwhile, the expression level of some key genes in hepatic lipid metabolism dysregulated significantly in the thiram-exposed chickens.
33010593	8	69	dep	decreased	1551:1559	arg1	whereas					1581:1587	whereas	1581:1587	whereas	1581:1587	In addition, some microbial populations including Lactobacillus, Ruminococcus, Oscillospira, Blautia, and Butyricicoccus significantly decreased at the genus level, whereas the Klebsiella was opposite.
33010593	5	70	theme	histopathological	911:927	arg1	examination					929:939	histopathological examination	911:939	histopathological examination	911:939	The results showed that thiram exposure prominently elevated liver index, changed liver function by histopathological examination and serum biochemistry diagnoses, and increased blood lipid parameters.
33010593	2	71	theme	lipid	317:321	arg1	metabolism					323:332	lipid metabolism	317:332	lipid metabolism by perturbing gut microbiota of chickens	317:373	However, the effects of thiram on lipid metabolism by perturbing gut microbiota of chickens are not clear.
33010593	10	72	dep	gut	1818:1820	arg1	the					1814:1816	the	1814:1816	the	1814:1816	Optimistically, 500 mg/kg MOP can alleviate the damage of thiram in the gut and liver.
33010593	0	73	theme	lipid	66:70	arg1	disorder					83:90	lipid metabolism disorder	66:90	lipid metabolism disorder in chickens	66:102	The fungicide thiram perturbs gut microbiota community and causes lipid metabolism disorder in chickens.
33010593	7	74	dep	richness	1247:1254	arg1	the					1243:1245	the	1243:1245	the	1243:1245	Furthermore, 16S rRNA gene sequencing indicated that thiram exposure can significantly alter the richness, diversity, and composition of the broiler fecal microbiota, and the relative abundance of Firmicutes and Proteobacteria was also affected at the phylum level.
33010593	2	75	theme	chickens	366:373	arg1	microbiota					352:361	gut microbiota	348:361	gut microbiota of chickens	348:373	However, the effects of thiram on lipid metabolism by perturbing gut microbiota of chickens are not clear.
33010593	3	76	theme	protective	425:434	arg1	polysaccharide					439:452	the protective of polysaccharide	421:452	the protective of polysaccharide extracted from Morinda officinalis (MOP) on acute thiram-exposed chickens	421:526	Our study was aimed to explore the protective of polysaccharide extracted from Morinda officinalis (MOP) on acute thiram-exposed chickens, and to analyze the association between alteration of gut microbiota and lipid metabolism.
33010593	7	77	theme	rRNA	1167:1170	arg1	sequencing					1177:1186	16S rRNA gene sequencing	1163:1186	16S rRNA gene sequencing	1163:1186	Furthermore, 16S rRNA gene sequencing indicated that thiram exposure can significantly alter the richness, diversity, and composition of the broiler fecal microbiota, and the relative abundance of Firmicutes and Proteobacteria was also affected at the phylum level.
33010593	3	78	theme	lipid	601:605	arg1	metabolism					607:616	lipid metabolism	601:616	lipid metabolism	601:616	Our study was aimed to explore the protective of polysaccharide extracted from Morinda officinalis (MOP) on acute thiram-exposed chickens, and to analyze the association between alteration of gut microbiota and lipid metabolism.
33010593	3	79	theme	thiram-exposed	504:517	arg1	chickens					519:526	acute thiram-exposed chickens	498:526	acute thiram-exposed chickens	498:526	Our study was aimed to explore the protective of polysaccharide extracted from Morinda officinalis (MOP) on acute thiram-exposed chickens, and to analyze the association between alteration of gut microbiota and lipid metabolism.
32246030	0	0	theme	polar	76:80	arg1	metabolites					82:92	polar metabolites	76:92	polar metabolites	76:92	Historical changes in the contents and compositions of fibre components and polar metabolites in white wheat flour.
32246030	3	1	theme	older	921:925	arg1	types					937:941	the older and newer types	917:941	the older and newer types	917:941	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	0	2	from	changes	11:17	arg1	compositions					39:50	compositions	39:50	compositions	39:50	Historical changes in the contents and compositions of fibre components and polar metabolites in white wheat flour.
32246030	0	2	from	changes	11:17	arg1	contents					26:33	contents	26:33	contents	26:33	Historical changes in the contents and compositions of fibre components and polar metabolites in white wheat flour.
32246030	1	3	theme	amino	392:396	arg1	acids					398:402	amino acids	392:402	amino acids	392:402	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	3	4	theme	acids	807:811	arg1	concentrations					685:698	the concentrations	681:698	the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types	681:941	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	5	5	theme	beneficial	1140:1149	arg1	components					1151:1160	beneficial components	1140:1160	beneficial components in wheat flour	1140:1175	The study therefore provided no evidence for adverse effects of intensive breeding on the contents of beneficial components in wheat flour.
32246030	1	6	theme	fibre	316:320	arg1	β-glucan					351:358	β-glucan	351:358	β-glucan	351:358	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	1	6	theme	fibre	316:320	arg1	arabinoxylan					334:345	arabinoxylan	334:345	arabinoxylan	334:345	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	1	6	theme	fibre	316:320	arg1	components					322:331	dietary fibre components	308:331	dietary fibre components (arabinoxylan and β-glucan)	308:359	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	1	7	dep	metabolites	371:381	arg1	sugars					384:389	sugars	384:389	sugars	384:389	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	1	7	dep	metabolites	371:381	arg1	betaine					432:438	betaine	432:438	betaine	432:438	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	1	7	dep	metabolites	371:381	arg1	choline					420:426	choline	420:426	choline	420:426	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	1	7	dep	metabolites	371:381	arg1	acids					398:402	amino acids	392:402	amino acids	392:402	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	1	7	dep	metabolites	371:381	arg1	acids					413:417	organic acids	405:417	organic acids	405:417	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	0	8	theme	metabolites	82:92	arg1	compositions					39:50	compositions	39:50	compositions	39:50	Historical changes in the contents and compositions of fibre components and polar metabolites in white wheat flour.
32246030	0	8	theme	metabolites	82:92	arg1	contents					26:33	contents	26:33	contents	26:33	Historical changes in the contents and compositions of fibre components and polar metabolites in white wheat flour.
32246030	1	9	theme	components	322:331	arg1	contents					296:303	the contents	292:303	the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine)	292:439	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	5	10	from	effects	1091:1097	arg1	contents					1128:1135	the contents	1124:1135	the contents of beneficial components in wheat flour	1124:1175	The study therefore provided no evidence for adverse effects of intensive breeding on the contents of beneficial components in wheat flour.
32246030	0	11	theme	wheat	103:107	arg1	flour					109:113	white wheat flour	97:113	white wheat flour	97:113	Historical changes in the contents and compositions of fibre components and polar metabolites in white wheat flour.
32246030	0	12	from	compositions	39:50	arg1	flour					109:113	white wheat flour	97:113	white wheat flour	97:113	Historical changes in the contents and compositions of fibre components and polar metabolites in white wheat flour.
32246030	2	13	theme	study	580:584	arg1	years					586:590	study years	580:590	study years	580:590	The concentrations of components varied between study years, indicating strong effects of environment.
32246030	1	14	theme	organic	405:411	arg1	acids					413:417	organic acids	405:417	organic acids	405:417	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	0	15	theme	white	97:101	arg1	flour					109:113	white wheat flour	97:113	white wheat flour	97:113	Historical changes in the contents and compositions of fibre components and polar metabolites in white wheat flour.
32246030	5	16	from	contents	1128:1135	arg1	flour					1171:1175	wheat flour	1165:1175	wheat flour	1165:1175	The study therefore provided no evidence for adverse effects of intensive breeding on the contents of beneficial components in wheat flour.
32246030	3	17	dep	acids	807:811	arg1	including					814:822	including	814:822	including asparagine which is the precursor of acrylamide formed during processing	814:895	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	0	18	theme	Historical	0:9	arg1	changes					11:17	Historical changes	0:17	Historical changes in the contents and compositions of fibre components and polar metabolites in white wheat flour.	0:114	Historical changes in the contents and compositions of fibre components and polar metabolites in white wheat flour.
32246030	5	19	theme	wheat	1165:1169	arg1	flour					1171:1175	wheat flour	1165:1175	wheat flour	1165:1175	The study therefore provided no evidence for adverse effects of intensive breeding on the contents of beneficial components in wheat flour.
32246030	3	20	theme	fibre	716:720	arg1	sucrose					750:756	sucrose	750:756	sucrose	750:756	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	3	20	theme	fibre	716:720	arg1	fructose					771:778	fructose	771:778	fructose	771:778	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	3	20	theme	fibre	716:720	arg1	maltose					759:765	maltose	759:765	maltose	759:765	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	3	20	theme	fibre	716:720	arg1	sugars					734:739	arabinoxylan fibre and soluble sugars	703:739	arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose)	703:779	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	4	21	theme	betaine	965:971	arg1	concentration					948:960	The concentration	944:960	The concentration of betaine, which is beneficial for cardio-vascular health,	944:1020	The concentration of betaine, which is beneficial for cardio-vascular health, also increased.
32246030	0	22	from	contents	26:33	arg1	flour					109:113	white wheat flour	97:113	white wheat flour	97:113	Historical changes in the contents and compositions of fibre components and polar metabolites in white wheat flour.
32246030	2	23	theme	environment	622:632	arg1	effects					611:617	strong effects	604:617	strong effects of environment	604:632	The concentrations of components varied between study years, indicating strong effects of environment.
32246030	1	24	theme	dietary	308:314	arg1	β-glucan					351:358	β-glucan	351:358	β-glucan	351:358	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	1	24	theme	dietary	308:314	arg1	arabinoxylan					334:345	arabinoxylan	334:345	arabinoxylan	334:345	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	1	24	theme	dietary	308:314	arg1	components					322:331	dietary fibre components	308:331	dietary fibre components (arabinoxylan and β-glucan)	308:359	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	5	25	theme	breeding	1112:1119	arg1	effects					1091:1097	adverse effects	1083:1097	adverse effects of intensive breeding on the contents of beneficial components in wheat flour	1083:1175	The study therefore provided no evidence for adverse effects of intensive breeding on the contents of beneficial components in wheat flour.
32246030	2	26	theme	components	554:563	arg1	concentrations					536:549	The concentrations	532:549	The concentrations of components	532:563	The concentrations of components varied between study years, indicating strong effects of environment.
32246030	3	27	theme	arabinoxylan	703:714	arg1	sucrose					750:756	sucrose	750:756	sucrose	750:756	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	3	27	theme	arabinoxylan	703:714	arg1	fructose					771:778	fructose	771:778	fructose	771:778	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	3	27	theme	arabinoxylan	703:714	arg1	maltose					759:765	maltose	759:765	maltose	759:765	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	3	27	theme	arabinoxylan	703:714	arg1	sugars					734:739	arabinoxylan fibre and soluble sugars	703:739	arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose)	703:779	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	5	28	from	components	1151:1160	arg1	flour					1171:1175	wheat flour	1165:1175	wheat flour	1165:1175	The study therefore provided no evidence for adverse effects of intensive breeding on the contents of beneficial components in wheat flour.
32246030	2	29	theme	strong	604:609	arg1	effects					611:617	strong effects	604:617	strong effects of environment	604:632	The concentrations of components varied between study years, indicating strong effects of environment.
32246030	3	30	theme	acrylamide	861:870	arg1	precursor					848:856	the precursor	844:856	the precursor of acrylamide formed during processing	844:895	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	3	30	theme	acrylamide	861:870	arg1	asparagine					824:833	asparagine	824:833	asparagine which is the precursor of acrylamide formed during processing	824:895	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	1	31	theme	intensive	512:520	arg1	breeding					522:529	intensive breeding	512:529	intensive breeding	512:529	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	1	32	theme	white	267:271	arg1	flour					273:277	white flour	267:277	white flour	267:277	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	4	33	theme	cardio-vascular	998:1012	arg1	health					1014:1019	cardio-vascular health	998:1019	cardio-vascular health	998:1019	The concentration of betaine, which is beneficial for cardio-vascular health, also increased.
32246030	1	34	theme	breeding	522:529	arg1	effects					501:507	the effects	497:507	the effects of intensive breeding	497:529	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	3	35	dep	sugars	734:739	arg1	sucrose					750:756	sucrose	750:756	sucrose	750:756	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	3	35	dep	sugars	734:739	arg1	fructose					771:778	fructose	771:778	fructose	771:778	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	3	35	dep	sugars	734:739	arg1	maltose					759:765	maltose	759:765	maltose	759:765	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	3	35	dep	sugars	734:739	arg1	sugars					734:739	arabinoxylan fibre and soluble sugars	703:739	arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose)	703:779	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	3	36	theme	soluble	726:732	arg1	sucrose					750:756	sucrose	750:756	sucrose	750:756	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	3	36	theme	soluble	726:732	arg1	fructose					771:778	fructose	771:778	fructose	771:778	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	3	36	theme	soluble	726:732	arg1	maltose					759:765	maltose	759:765	maltose	759:765	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	3	36	theme	soluble	726:732	arg1	sugars					734:739	arabinoxylan fibre and soluble sugars	703:739	arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose)	703:779	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	3	37	theme	sugars	734:739	arg1	concentrations					685:698	the concentrations	681:698	the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types	681:941	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	1	38	theme	polar	365:369	arg1	metabolites					371:381	polar metabolites	365:381	polar metabolites (sugars, amino acids, organic acids, choline and betaine)	365:439	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	1	39	dep	components	322:331	arg1	β-glucan					351:358	β-glucan	351:358	β-glucan	351:358	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	1	39	dep	components	322:331	arg1	arabinoxylan					334:345	arabinoxylan	334:345	arabinoxylan	334:345	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	1	39	dep	components	322:331	arg1	components					322:331	dietary fibre components	308:331	dietary fibre components (arabinoxylan and β-glucan)	308:359	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	1	40	theme	metabolites	371:381	arg1	contents					296:303	the contents	292:303	the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine)	292:439	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	0	41	dep	contents	26:33	arg1	the					22:24	the	22:24	the	22:24	Historical changes in the contents and compositions of fibre components and polar metabolites in white wheat flour.
32246030	5	42	theme	adverse	1083:1089	arg1	effects					1091:1097	adverse effects	1083:1097	adverse effects of intensive breeding on the contents of beneficial components in wheat flour	1083:1175	The study therefore provided no evidence for adverse effects of intensive breeding on the contents of beneficial components in wheat flour.
32246030	3	43	theme	most	796:799	arg1	acids					807:811	most amino acids	796:811	most amino acids (including asparagine which is the precursor of acrylamide formed during processing)	796:896	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	5	44	theme	intensive	1102:1110	arg1	breeding					1112:1119	intensive breeding	1102:1119	intensive breeding	1102:1119	The study therefore provided no evidence for adverse effects of intensive breeding on the contents of beneficial components in wheat flour.
32246030	5	45	theme	components	1151:1160	arg1	contents					1128:1135	the contents	1124:1135	the contents of beneficial components in wheat flour	1124:1175	The study therefore provided no evidence for adverse effects of intensive breeding on the contents of beneficial components in wheat flour.
32246030	1	46	theme	Thirty-nine	116:126	arg1	cultivars					145:153	Thirty-nine UK adapted wheat cultivars	116:153	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012	116:187	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	3	47	theme	amino	801:805	arg1	acids					807:811	most amino acids	796:811	most amino acids (including asparagine which is the precursor of acrylamide formed during processing)	796:896	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	1	48	theme	replicated	203:212	arg1	trials					231:236	replicated randomised field trials	203:236	replicated randomised field trials	203:236	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	5	49	from	flour	1171:1175	arg1	contents					1128:1135	the contents	1124:1135	the contents of beneficial components in wheat flour	1124:1175	The study therefore provided no evidence for adverse effects of intensive breeding on the contents of beneficial components in wheat flour.
32246030	1	50	theme	UK	128:129	arg1	cultivars					145:153	Thirty-nine UK adapted wheat cultivars	116:153	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012	116:187	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	3	51	theme	increasing	781:790	arg1	sucrose					750:756	sucrose	750:756	sucrose	750:756	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	3	51	theme	increasing	781:790	arg1	fructose					771:778	fructose	771:778	fructose	771:778	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	3	51	theme	increasing	781:790	arg1	maltose					759:765	maltose	759:765	maltose	759:765	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	3	51	theme	increasing	781:790	arg1	sugars					734:739	arabinoxylan fibre and soluble sugars	703:739	arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose)	703:779	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	1	52	theme	randomised	214:223	arg1	trials					231:236	replicated randomised field trials	203:236	replicated randomised field trials	203:236	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	0	53	theme	components	61:70	arg1	compositions					39:50	compositions	39:50	compositions	39:50	Historical changes in the contents and compositions of fibre components and polar metabolites in white wheat flour.
32246030	0	53	theme	components	61:70	arg1	contents					26:33	contents	26:33	contents	26:33	Historical changes in the contents and compositions of fibre components and polar metabolites in white wheat flour.
32246030	3	54	theme	newer	931:935	arg1	types					937:941	the older and newer types	917:941	the older and newer types	917:941	Nevertheless, some trends were observed, with the concentrations of arabinoxylan fibre and soluble sugars (notably sucrose, maltose and fructose) increasing and most amino acids (including asparagine which is the precursor of acrylamide formed during processing) decreasing between the older and newer types.
32246030	1	55	theme	adapted	131:137	arg1	cultivars					145:153	Thirty-nine UK adapted wheat cultivars	116:153	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012	116:187	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	1	56	theme	field	225:229	arg1	trials					231:236	replicated randomised field trials	203:236	replicated randomised field trials	203:236	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
32246030	0	57	theme	fibre	55:59	arg1	components					61:70	fibre components	55:70	fibre components	55:70	Historical changes in the contents and compositions of fibre components and polar metabolites in white wheat flour.
32246030	1	58	theme	wheat	139:143	arg1	cultivars					145:153	Thirty-nine UK adapted wheat cultivars	116:153	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012	116:187	Thirty-nine UK adapted wheat cultivars dating from between 1790 and 2012 were grown in replicated randomised field trials for three years, milled, and white flour analysed for the contents of dietary fibre components (arabinoxylan and β-glucan) and polar metabolites (sugars, amino acids, organic acids, choline and betaine) to determine whether the composition had changed due to the effects of intensive breeding.
34554172	4	0	theme	JRP	1114:1116	arg1	biodegradation					1096:1109	the biodegradation	1092:1109	the biodegradation of JRP	1092:1116	Through measurements of gastrointestinal digestion and colonic fermentation in vitro, the results suggest that BSA-JRP-HSP NPs presented well-sustained release characteristics for preventing the biodegradation of JRP during gastrointestinal digestion.
34554172	3	1	theme	average	732:738	arg1	size					749:752	average particle size	732:752	average particle size of 285.7 ± 3.1 nm	732:770	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	0	2	theme	colonic	195:201	arg1	fermentation					203:214	colonic fermentation	195:214	colonic fermentation in vitro	195:223	Construction and characterization of Juglans regia L. polyphenols nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides, and their gastrointestinal digestion and colonic fermentation in vitro.
34554172	0	2	theme	colonic	195:201	arg1	polysaccharides					137:151	bovine serum albumin and Hohenbuehelia serotina polysaccharides	89:151	bovine serum albumin and Hohenbuehelia serotina polysaccharides	89:151	Construction and characterization of Juglans regia L. polyphenols nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides, and their gastrointestinal digestion and colonic fermentation in vitro.
34554172	4	3	theme	gastrointestinal	1125:1140	arg1	digestion					1142:1150	gastrointestinal digestion	1125:1150	gastrointestinal digestion	1125:1150	Through measurements of gastrointestinal digestion and colonic fermentation in vitro, the results suggest that BSA-JRP-HSP NPs presented well-sustained release characteristics for preventing the biodegradation of JRP during gastrointestinal digestion.
34554172	3	4	theme	3.1	765:767	arg1	±					763:763	±	763:763	±	763:763	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	3	5	theme	285.7	757:761	arg1	±					763:763	±	763:763	±	763:763	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	3	6	theme	BSA-JRP-HSP	588:598	arg1	NPs					600:602	BSA-JRP-HSP NPs	588:602	BSA-JRP-HSP NPs	588:602	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	3	7	theme	zeta	777:780	arg1	potential					782:790	zeta potential	777:790	zeta potential of -12.20 ± 0.61 mV	777:810	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	6	8	theme	butyric	1582:1588	arg1	acid					1590:1593	butyric acid	1582:1593	butyric acid	1582:1593	The production of short-chain fatty acids (SCFAs) including acetic acid, propionic acid, and butyric acid was remarkably enhanced by treatment with BSA-JRP-HSP NPs.
34554172	4	9	theme	fermentation	964:975	arg1	measurements					909:920	measurements	909:920	measurements of gastrointestinal digestion and colonic fermentation in vitro	909:984	Through measurements of gastrointestinal digestion and colonic fermentation in vitro, the results suggest that BSA-JRP-HSP NPs presented well-sustained release characteristics for preventing the biodegradation of JRP during gastrointestinal digestion.
34554172	5	10	dep	Akkermansia	1341:1351	arg1	e.g.					1313:1316	e.g.	1313:1316	e.g.	1313:1316	After gastrointestinal digestion, BSA-JRP-HSP NPs could modulate the composition and structure of gut microbiota, promoting the growth of beneficial bacterial (e.g. Prevotella, Dialister, Akkermansia, etc.) and inhibiting the growth of pathogenic bacteria (e.g. Bacteroides, Phascolarctobacterium, Lachnospiracea incertae sedis, etc.).
34554172	0	11	theme	serotina	128:135	arg1	digestion					181:189	their gastrointestinal digestion	158:189	their gastrointestinal digestion	158:189	Construction and characterization of Juglans regia L. polyphenols nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides, and their gastrointestinal digestion and colonic fermentation in vitro.
34554172	0	11	theme	serotina	128:135	arg1	polysaccharides					137:151	bovine serum albumin and Hohenbuehelia serotina polysaccharides	89:151	bovine serum albumin and Hohenbuehelia serotina polysaccharides	89:151	Construction and characterization of Juglans regia L. polyphenols nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides, and their gastrointestinal digestion and colonic fermentation in vitro.
34554172	0	11	theme	serotina	128:135	arg1	fermentation					203:214	colonic fermentation	195:214	colonic fermentation in vitro	195:223	Construction and characterization of Juglans regia L. polyphenols nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides, and their gastrointestinal digestion and colonic fermentation in vitro.
34554172	3	12	theme	amorphous	610:618	arg1	properties					620:629	amorphous properties	610:629	amorphous properties	610:629	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	5	13	theme	gastrointestinal	1159:1174	arg1	digestion					1176:1184	gastrointestinal digestion	1159:1184	gastrointestinal digestion	1159:1184	After gastrointestinal digestion, BSA-JRP-HSP NPs could modulate the composition and structure of gut microbiota, promoting the growth of beneficial bacterial (e.g. Prevotella, Dialister, Akkermansia, etc.) and inhibiting the growth of pathogenic bacteria (e.g. Bacteroides, Phascolarctobacterium, Lachnospiracea incertae sedis, etc.).
34554172	7	14	theme	BSA-JRP-HSP	1681:1691	arg1	candidate					1718:1726	a novel candidate	1710:1726	a novel candidate for improving the bioavailability of JRP	1710:1767	This study has proved that BSA-JRP-HSP NPs can serve as a novel candidate for improving the bioavailability of JRP.
34554172	7	14	theme	BSA-JRP-HSP	1681:1691	arg1	NPs					1693:1695	BSA-JRP-HSP NPs	1681:1695	BSA-JRP-HSP NPs	1681:1695	This study has proved that BSA-JRP-HSP NPs can serve as a novel candidate for improving the bioavailability of JRP.
34554172	4	15	theme	gastrointestinal	925:940	arg1	digestion					942:950	gastrointestinal digestion	925:950	gastrointestinal digestion	925:950	Through measurements of gastrointestinal digestion and colonic fermentation in vitro, the results suggest that BSA-JRP-HSP NPs presented well-sustained release characteristics for preventing the biodegradation of JRP during gastrointestinal digestion.
34554172	3	16	theme	spherical	643:651	arg1	features					667:674	regular spherical morphological features	635:674	regular spherical morphological features	635:674	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	3	17	theme	mucin	876:880	arg1	capacity					891:898	strong mucin adhesion capacity	869:898	strong mucin adhesion capacity	869:898	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	3	18	theme	high	684:687	arg1	efficiency					703:712	a high encapsulation efficiency	682:712	a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV	682:810	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	6	19	theme	BSA-JRP-HSP	1637:1647	arg1	NPs					1649:1651	BSA-JRP-HSP NPs	1637:1651	BSA-JRP-HSP NPs	1637:1651	The production of short-chain fatty acids (SCFAs) including acetic acid, propionic acid, and butyric acid was remarkably enhanced by treatment with BSA-JRP-HSP NPs.
34554172	4	20	theme	BSA-JRP-HSP	1012:1022	arg1	NPs					1024:1026	BSA-JRP-HSP NPs	1012:1026	BSA-JRP-HSP NPs	1012:1026	Through measurements of gastrointestinal digestion and colonic fermentation in vitro, the results suggest that BSA-JRP-HSP NPs presented well-sustained release characteristics for preventing the biodegradation of JRP during gastrointestinal digestion.
34554172	3	21	theme	mV	809:810	arg1	size					749:752	average particle size	732:752	average particle size of 285.7 ± 3.1 nm	732:770	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	3	21	theme	mV	809:810	arg1	potential					782:790	zeta potential	777:790	zeta potential of -12.20 ± 0.61 mV	777:810	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	3	21	theme	mV	809:810	arg1	±					723:723	88.47 ±	717:723	88.47 ± 0.04%	717:729	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	5	22	theme	bacterial	1302:1310	arg1	growth					1281:1286	the growth	1277:1286	the growth of beneficial bacterial (e.g. Prevotella, Dialister, Akkermansia, etc.)	1277:1358	After gastrointestinal digestion, BSA-JRP-HSP NPs could modulate the composition and structure of gut microbiota, promoting the growth of beneficial bacterial (e.g. Prevotella, Dialister, Akkermansia, etc.) and inhibiting the growth of pathogenic bacteria (e.g. Bacteroides, Phascolarctobacterium, Lachnospiracea incertae sedis, etc.).
34554172	3	23	theme	photothermal	840:851	arg1	stabilities					853:863	excellent photothermal stabilities	830:863	excellent photothermal stabilities	830:863	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	7	24	theme	JRP	1765:1767	arg1	bioavailability					1746:1760	the bioavailability	1742:1760	the bioavailability of JRP	1742:1767	This study has proved that BSA-JRP-HSP NPs can serve as a novel candidate for improving the bioavailability of JRP.
34554172	0	25	theme	serum	96:100	arg1	albumin					102:108	bovine serum albumin	89:108	bovine serum albumin	89:108	Construction and characterization of Juglans regia L. polyphenols nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides, and their gastrointestinal digestion and colonic fermentation in vitro.
34554172	1	26	theme	polyphenols	392:402	arg1	delivery					380:387	the delivery	376:387	the delivery of polyphenols isolated from the shells of Juglans regia L. (BSA-JRP-HSP NPs)	376:465	Herein, we report the construction and characterization of nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides for the delivery of polyphenols isolated from the shells of Juglans regia L. (BSA-JRP-HSP NPs).
34554172	2	27	theme	colonic	541:547	arg1	characteristics					562:576	colonic fermentation characteristics	541:576	colonic fermentation characteristics	541:576	We also systematically investigated their gastrointestinal digestion and colonic fermentation characteristics in vitro.
34554172	6	28	theme	fatty	1519:1523	arg1	acid					1556:1559	acetic acid	1549:1559	acetic acid	1549:1559	The production of short-chain fatty acids (SCFAs) including acetic acid, propionic acid, and butyric acid was remarkably enhanced by treatment with BSA-JRP-HSP NPs.
34554172	6	28	theme	fatty	1519:1523	arg1	acid					1572:1575	propionic acid	1562:1575	propionic acid	1562:1575	The production of short-chain fatty acids (SCFAs) including acetic acid, propionic acid, and butyric acid was remarkably enhanced by treatment with BSA-JRP-HSP NPs.
34554172	6	28	theme	fatty	1519:1523	arg1	SCFAs					1532:1536	SCFAs	1532:1536	SCFAs	1532:1536	The production of short-chain fatty acids (SCFAs) including acetic acid, propionic acid, and butyric acid was remarkably enhanced by treatment with BSA-JRP-HSP NPs.
34554172	6	28	theme	fatty	1519:1523	arg1	acid					1590:1593	butyric acid	1582:1593	butyric acid	1582:1593	The production of short-chain fatty acids (SCFAs) including acetic acid, propionic acid, and butyric acid was remarkably enhanced by treatment with BSA-JRP-HSP NPs.
34554172	6	28	theme	fatty	1519:1523	arg1	acids					1525:1529	short-chain fatty acids	1507:1529	short-chain fatty acids (SCFAs) including acetic acid, propionic acid, and butyric acid	1507:1593	The production of short-chain fatty acids (SCFAs) including acetic acid, propionic acid, and butyric acid was remarkably enhanced by treatment with BSA-JRP-HSP NPs.
34554172	5	29	dep	Lachnospiracea	1451:1464	arg1	sedis					1475:1479	Lachnospiracea incertae sedis	1451:1479	Lachnospiracea incertae sedis	1451:1479	After gastrointestinal digestion, BSA-JRP-HSP NPs could modulate the composition and structure of gut microbiota, promoting the growth of beneficial bacterial (e.g. Prevotella, Dialister, Akkermansia, etc.) and inhibiting the growth of pathogenic bacteria (e.g. Bacteroides, Phascolarctobacterium, Lachnospiracea incertae sedis, etc.).
34554172	1	30	theme	serum	315:319	arg1	albumin					321:327	bovine serum albumin	308:327	bovine serum albumin	308:327	Herein, we report the construction and characterization of nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides for the delivery of polyphenols isolated from the shells of Juglans regia L. (BSA-JRP-HSP NPs).
34554172	0	31	dep	nanoparticles	66:78	arg1	based					80:84	based	80:84	nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides, and their gastrointestinal digestion and colonic fermentation in vitro	66:223	Construction and characterization of Juglans regia L. polyphenols nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides, and their gastrointestinal digestion and colonic fermentation in vitro.
34554172	5	32	dep	Phascolarctobacterium	1428:1448	arg1	e.g.					1410:1413	e.g.	1410:1413	e.g.	1410:1413	After gastrointestinal digestion, BSA-JRP-HSP NPs could modulate the composition and structure of gut microbiota, promoting the growth of beneficial bacterial (e.g. Prevotella, Dialister, Akkermansia, etc.) and inhibiting the growth of pathogenic bacteria (e.g. Bacteroides, Phascolarctobacterium, Lachnospiracea incertae sedis, etc.).
34554172	4	33	theme	release	1053:1059	arg1	characteristics					1061:1075	well-sustained release characteristics	1038:1075	well-sustained release characteristics	1038:1075	Through measurements of gastrointestinal digestion and colonic fermentation in vitro, the results suggest that BSA-JRP-HSP NPs presented well-sustained release characteristics for preventing the biodegradation of JRP during gastrointestinal digestion.
34554172	5	34	theme	bacteria	1400:1407	arg1	growth					1379:1384	the growth	1375:1384	the growth of pathogenic bacteria (e.g. Bacteroides, Phascolarctobacterium, Lachnospiracea incertae sedis, etc.)	1375:1486	After gastrointestinal digestion, BSA-JRP-HSP NPs could modulate the composition and structure of gut microbiota, promoting the growth of beneficial bacterial (e.g. Prevotella, Dialister, Akkermansia, etc.) and inhibiting the growth of pathogenic bacteria (e.g. Bacteroides, Phascolarctobacterium, Lachnospiracea incertae sedis, etc.).
34554172	0	35	theme	Juglans	37:43	arg1	polyphenols					54:64	Juglans regia L. polyphenols	37:64	Juglans regia L. polyphenols	37:64	Construction and characterization of Juglans regia L. polyphenols nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides, and their gastrointestinal digestion and colonic fermentation in vitro.
34554172	5	36	theme	gut	1251:1253	arg1	microbiota					1255:1264	gut microbiota	1251:1264	gut microbiota	1251:1264	After gastrointestinal digestion, BSA-JRP-HSP NPs could modulate the composition and structure of gut microbiota, promoting the growth of beneficial bacterial (e.g. Prevotella, Dialister, Akkermansia, etc.) and inhibiting the growth of pathogenic bacteria (e.g. Bacteroides, Phascolarctobacterium, Lachnospiracea incertae sedis, etc.).
34554172	0	37	dep	Juglans	37:43	arg1	L.					51:52	Juglans regia L.	37:52	Juglans regia L. polyphenols	37:64	Construction and characterization of Juglans regia L. polyphenols nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides, and their gastrointestinal digestion and colonic fermentation in vitro.
34554172	1	38	dep	regia	440:444	arg1	L					446:446	L	446:446	Juglans regia L. (BSA-JRP-HSP NPs)	432:465	Herein, we report the construction and characterization of nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides for the delivery of polyphenols isolated from the shells of Juglans regia L. (BSA-JRP-HSP NPs).
34554172	3	39	theme	0.04	725:728	arg1	%					729:729	%	729:729	%	729:729	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	1	40	attach	isolated	404:411	arg1	shells					422:427	the shells	418:427	the shells of Juglans regia L. (BSA-JRP-HSP NPs)	418:465	Herein, we report the construction and characterization of nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides for the delivery of polyphenols isolated from the shells of Juglans regia L. (BSA-JRP-HSP NPs).
34554172	1	40	attach	isolated	404:411	arg2	polyphenols					392:402	polyphenols	392:402	polyphenols isolated from the shells of Juglans regia L. (BSA-JRP-HSP NPs)	392:465	Herein, we report the construction and characterization of nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides for the delivery of polyphenols isolated from the shells of Juglans regia L. (BSA-JRP-HSP NPs).
34554172	6	41	theme	acetic	1549:1554	arg1	acid					1556:1559	acetic acid	1549:1559	acetic acid	1549:1559	The production of short-chain fatty acids (SCFAs) including acetic acid, propionic acid, and butyric acid was remarkably enhanced by treatment with BSA-JRP-HSP NPs.
34554172	3	42	with	NPs	600:602	arg1	features					667:674	regular spherical morphological features	635:674	regular spherical morphological features	635:674	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	3	42	with	NPs	600:602	arg1	properties					620:629	amorphous properties	610:629	amorphous properties	610:629	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	3	43	theme	particle	740:747	arg1	size					749:752	average particle size	732:752	average particle size of 285.7 ± 3.1 nm	732:770	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	1	44	theme	nanoparticles	285:297	arg1	construction					248:259	construction	248:259	construction	248:259	Herein, we report the construction and characterization of nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides for the delivery of polyphenols isolated from the shells of Juglans regia L. (BSA-JRP-HSP NPs).
34554172	1	44	theme	nanoparticles	285:297	arg1	characterization					265:280	characterization	265:280	characterization	265:280	Herein, we report the construction and characterization of nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides for the delivery of polyphenols isolated from the shells of Juglans regia L. (BSA-JRP-HSP NPs).
34554172	3	45	theme	nm	769:770	arg1	size					749:752	average particle size	732:752	average particle size of 285.7 ± 3.1 nm	732:770	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	3	45	theme	nm	769:770	arg1	potential					782:790	zeta potential	777:790	zeta potential of -12.20 ± 0.61 mV	777:810	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	3	45	theme	nm	769:770	arg1	±					723:723	88.47 ±	717:723	88.47 ± 0.04%	717:729	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	1	46	theme	serotina	347:354	arg1	polysaccharides					356:370	bovine serum albumin and Hohenbuehelia serotina polysaccharides	308:370	bovine serum albumin and Hohenbuehelia serotina polysaccharides for the delivery of polyphenols isolated from the shells of Juglans regia L. (BSA-JRP-HSP NPs)	308:465	Herein, we report the construction and characterization of nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides for the delivery of polyphenols isolated from the shells of Juglans regia L. (BSA-JRP-HSP NPs).
34554172	3	47	theme	potential	782:790	arg1	efficiency					703:712	a high encapsulation efficiency	682:712	a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV	682:810	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	4	48	theme	colonic	956:962	arg1	fermentation					964:975	colonic fermentation	956:975	colonic fermentation	956:975	Through measurements of gastrointestinal digestion and colonic fermentation in vitro, the results suggest that BSA-JRP-HSP NPs presented well-sustained release characteristics for preventing the biodegradation of JRP during gastrointestinal digestion.
34554172	1	49	dep	construction	248:259	arg1	the					244:246	the	244:246	the	244:246	Herein, we report the construction and characterization of nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides for the delivery of polyphenols isolated from the shells of Juglans regia L. (BSA-JRP-HSP NPs).
34554172	3	50	theme	morphological	653:665	arg1	features					667:674	regular spherical morphological features	635:674	regular spherical morphological features	635:674	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	6	51	with	treatment	1622:1630	arg1	NPs					1649:1651	BSA-JRP-HSP NPs	1637:1651	BSA-JRP-HSP NPs	1637:1651	The production of short-chain fatty acids (SCFAs) including acetic acid, propionic acid, and butyric acid was remarkably enhanced by treatment with BSA-JRP-HSP NPs.
34554172	0	52	theme	gastrointestinal	164:179	arg1	digestion					181:189	their gastrointestinal digestion	158:189	their gastrointestinal digestion	158:189	Construction and characterization of Juglans regia L. polyphenols nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides, and their gastrointestinal digestion and colonic fermentation in vitro.
34554172	0	52	theme	gastrointestinal	164:179	arg1	polysaccharides					137:151	bovine serum albumin and Hohenbuehelia serotina polysaccharides	89:151	bovine serum albumin and Hohenbuehelia serotina polysaccharides	89:151	Construction and characterization of Juglans regia L. polyphenols nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides, and their gastrointestinal digestion and colonic fermentation in vitro.
34554172	1	53	theme	regia	440:444	arg1	shells					422:427	the shells	418:427	the shells of Juglans regia L. (BSA-JRP-HSP NPs)	418:465	Herein, we report the construction and characterization of nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides for the delivery of polyphenols isolated from the shells of Juglans regia L. (BSA-JRP-HSP NPs).
34554172	3	54	theme	regular	635:641	arg1	features					667:674	regular spherical morphological features	635:674	regular spherical morphological features	635:674	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	3	55	theme	-12.20	795:800	arg1	±					802:802	±	802:802	±	802:802	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	3	56	theme	adhesion	882:889	arg1	capacity					891:898	strong mucin adhesion capacity	869:898	strong mucin adhesion capacity	869:898	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	4	57	theme	digestion	942:950	arg1	measurements					909:920	measurements	909:920	measurements of gastrointestinal digestion and colonic fermentation in vitro	909:984	Through measurements of gastrointestinal digestion and colonic fermentation in vitro, the results suggest that BSA-JRP-HSP NPs presented well-sustained release characteristics for preventing the biodegradation of JRP during gastrointestinal digestion.
34554172	1	58	theme	BSA-JRP-HSP	450:460	arg1	NPs					462:464	BSA-JRP-HSP NPs	450:464	BSA-JRP-HSP NPs	450:464	Herein, we report the construction and characterization of nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides for the delivery of polyphenols isolated from the shells of Juglans regia L. (BSA-JRP-HSP NPs).
34554172	1	58	theme	BSA-JRP-HSP	450:460	arg1	regia					440:444	regia	440:444	regia	440:444	Herein, we report the construction and characterization of nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides for the delivery of polyphenols isolated from the shells of Juglans regia L. (BSA-JRP-HSP NPs).
34554172	3	59	theme	strong	869:874	arg1	capacity					891:898	strong mucin adhesion capacity	869:898	strong mucin adhesion capacity	869:898	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	0	60	theme	bovine	89:94	arg1	albumin					102:108	bovine serum albumin	89:108	bovine serum albumin	89:108	Construction and characterization of Juglans regia L. polyphenols nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides, and their gastrointestinal digestion and colonic fermentation in vitro.
34554172	2	61	theme	gastrointestinal	510:525	arg1	digestion					527:535	their gastrointestinal digestion	504:535	their gastrointestinal digestion	504:535	We also systematically investigated their gastrointestinal digestion and colonic fermentation characteristics in vitro.
34554172	0	62	theme	albumin	102:108	arg1	digestion					181:189	their gastrointestinal digestion	158:189	their gastrointestinal digestion	158:189	Construction and characterization of Juglans regia L. polyphenols nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides, and their gastrointestinal digestion and colonic fermentation in vitro.
34554172	0	62	theme	albumin	102:108	arg1	polysaccharides					137:151	bovine serum albumin and Hohenbuehelia serotina polysaccharides	89:151	bovine serum albumin and Hohenbuehelia serotina polysaccharides	89:151	Construction and characterization of Juglans regia L. polyphenols nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides, and their gastrointestinal digestion and colonic fermentation in vitro.
34554172	0	62	theme	albumin	102:108	arg1	fermentation					203:214	colonic fermentation	195:214	colonic fermentation in vitro	195:223	Construction and characterization of Juglans regia L. polyphenols nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides, and their gastrointestinal digestion and colonic fermentation in vitro.
34554172	3	63	theme	excellent	830:838	arg1	stabilities					853:863	excellent photothermal stabilities	830:863	excellent photothermal stabilities	830:863	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	0	64	theme	Hohenbuehelia	114:126	arg1	serotina					128:135	Hohenbuehelia serotina	114:135	Hohenbuehelia serotina	114:135	Construction and characterization of Juglans regia L. polyphenols nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides, and their gastrointestinal digestion and colonic fermentation in vitro.
34554172	5	65	theme	BSA-JRP-HSP	1187:1197	arg1	NPs					1199:1201	BSA-JRP-HSP NPs	1187:1201	BSA-JRP-HSP NPs	1187:1201	After gastrointestinal digestion, BSA-JRP-HSP NPs could modulate the composition and structure of gut microbiota, promoting the growth of beneficial bacterial (e.g. Prevotella, Dialister, Akkermansia, etc.) and inhibiting the growth of pathogenic bacteria (e.g. Bacteroides, Phascolarctobacterium, Lachnospiracea incertae sedis, etc.).
34554172	2	66	theme	fermentation	549:560	arg1	characteristics					562:576	colonic fermentation characteristics	541:576	colonic fermentation characteristics	541:576	We also systematically investigated their gastrointestinal digestion and colonic fermentation characteristics in vitro.
34554172	6	67	theme	acids	1525:1529	arg1	production					1493:1502	The production	1489:1502	The production of short-chain fatty acids (SCFAs) including acetic acid, propionic acid, and butyric acid	1489:1593	The production of short-chain fatty acids (SCFAs) including acetic acid, propionic acid, and butyric acid was remarkably enhanced by treatment with BSA-JRP-HSP NPs.
34554172	5	68	dep	composition	1222:1232	arg1	the					1218:1220	the	1218:1220	the	1218:1220	After gastrointestinal digestion, BSA-JRP-HSP NPs could modulate the composition and structure of gut microbiota, promoting the growth of beneficial bacterial (e.g. Prevotella, Dialister, Akkermansia, etc.) and inhibiting the growth of pathogenic bacteria (e.g. Bacteroides, Phascolarctobacterium, Lachnospiracea incertae sedis, etc.).
34554172	6	69	theme	short-chain	1507:1517	arg1	acid					1556:1559	acetic acid	1549:1559	acetic acid	1549:1559	The production of short-chain fatty acids (SCFAs) including acetic acid, propionic acid, and butyric acid was remarkably enhanced by treatment with BSA-JRP-HSP NPs.
34554172	6	69	theme	short-chain	1507:1517	arg1	acid					1572:1575	propionic acid	1562:1575	propionic acid	1562:1575	The production of short-chain fatty acids (SCFAs) including acetic acid, propionic acid, and butyric acid was remarkably enhanced by treatment with BSA-JRP-HSP NPs.
34554172	6	69	theme	short-chain	1507:1517	arg1	SCFAs					1532:1536	SCFAs	1532:1536	SCFAs	1532:1536	The production of short-chain fatty acids (SCFAs) including acetic acid, propionic acid, and butyric acid was remarkably enhanced by treatment with BSA-JRP-HSP NPs.
34554172	6	69	theme	short-chain	1507:1517	arg1	acid					1590:1593	butyric acid	1582:1593	butyric acid	1582:1593	The production of short-chain fatty acids (SCFAs) including acetic acid, propionic acid, and butyric acid was remarkably enhanced by treatment with BSA-JRP-HSP NPs.
34554172	6	69	theme	short-chain	1507:1517	arg1	acids					1525:1529	short-chain fatty acids	1507:1529	short-chain fatty acids (SCFAs) including acetic acid, propionic acid, and butyric acid	1507:1593	The production of short-chain fatty acids (SCFAs) including acetic acid, propionic acid, and butyric acid was remarkably enhanced by treatment with BSA-JRP-HSP NPs.
34554172	1	70	theme	bovine	308:313	arg1	albumin					321:327	bovine serum albumin	308:327	bovine serum albumin	308:327	Herein, we report the construction and characterization of nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides for the delivery of polyphenols isolated from the shells of Juglans regia L. (BSA-JRP-HSP NPs).
34554172	5	71	dep	modulate	1209:1216	arg1	promoting					1267:1275	promoting	1267:1275	promoting the growth of beneficial bacterial (e.g. Prevotella, Dialister, Akkermansia, etc.)	1267:1358	After gastrointestinal digestion, BSA-JRP-HSP NPs could modulate the composition and structure of gut microbiota, promoting the growth of beneficial bacterial (e.g. Prevotella, Dialister, Akkermansia, etc.) and inhibiting the growth of pathogenic bacteria (e.g. Bacteroides, Phascolarctobacterium, Lachnospiracea incertae sedis, etc.).
34554172	5	71	dep	modulate	1209:1216	arg1	inhibiting					1364:1373	inhibiting	1364:1373	inhibiting the growth of pathogenic bacteria (e.g. Bacteroides, Phascolarctobacterium, Lachnospiracea incertae sedis, etc.)	1364:1486	After gastrointestinal digestion, BSA-JRP-HSP NPs could modulate the composition and structure of gut microbiota, promoting the growth of beneficial bacterial (e.g. Prevotella, Dialister, Akkermansia, etc.) and inhibiting the growth of pathogenic bacteria (e.g. Bacteroides, Phascolarctobacterium, Lachnospiracea incertae sedis, etc.).
34554172	5	72	theme	pathogenic	1389:1398	arg1	bacteria					1400:1407	pathogenic bacteria	1389:1407	pathogenic bacteria (e.g. Bacteroides, Phascolarctobacterium, Lachnospiracea incertae sedis, etc.)	1389:1486	After gastrointestinal digestion, BSA-JRP-HSP NPs could modulate the composition and structure of gut microbiota, promoting the growth of beneficial bacterial (e.g. Prevotella, Dialister, Akkermansia, etc.) and inhibiting the growth of pathogenic bacteria (e.g. Bacteroides, Phascolarctobacterium, Lachnospiracea incertae sedis, etc.).
34554172	1	73	theme	albumin	321:327	arg1	polysaccharides					356:370	bovine serum albumin and Hohenbuehelia serotina polysaccharides	308:370	bovine serum albumin and Hohenbuehelia serotina polysaccharides for the delivery of polyphenols isolated from the shells of Juglans regia L. (BSA-JRP-HSP NPs)	308:465	Herein, we report the construction and characterization of nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides for the delivery of polyphenols isolated from the shells of Juglans regia L. (BSA-JRP-HSP NPs).
34554172	7	74	theme	novel	1712:1716	arg1	candidate					1718:1726	a novel candidate	1710:1726	a novel candidate for improving the bioavailability of JRP	1710:1767	This study has proved that BSA-JRP-HSP NPs can serve as a novel candidate for improving the bioavailability of JRP.
34554172	7	74	theme	novel	1712:1716	arg1	NPs					1693:1695	BSA-JRP-HSP NPs	1681:1695	BSA-JRP-HSP NPs	1681:1695	This study has proved that BSA-JRP-HSP NPs can serve as a novel candidate for improving the bioavailability of JRP.
34554172	4	75	theme	well-sustained	1038:1051	arg1	characteristics					1061:1075	well-sustained release characteristics	1038:1075	well-sustained release characteristics	1038:1075	Through measurements of gastrointestinal digestion and colonic fermentation in vitro, the results suggest that BSA-JRP-HSP NPs presented well-sustained release characteristics for preventing the biodegradation of JRP during gastrointestinal digestion.
34554172	3	76	theme	encapsulation	689:701	arg1	efficiency					703:712	a high encapsulation efficiency	682:712	a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV	682:810	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	0	77	theme	polyphenols	54:64	arg1	characterization					17:32	characterization	17:32	characterization	17:32	Construction and characterization of Juglans regia L. polyphenols nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides, and their gastrointestinal digestion and colonic fermentation in vitro.
34554172	0	77	theme	polyphenols	54:64	arg1	Construction					0:11	Construction	0:11	Construction	0:11	Construction and characterization of Juglans regia L. polyphenols nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides, and their gastrointestinal digestion and colonic fermentation in vitro.
34554172	1	78	theme	Hohenbuehelia	333:345	arg1	serotina					347:354	Hohenbuehelia serotina	333:354	Hohenbuehelia serotina	333:354	Herein, we report the construction and characterization of nanoparticles based on bovine serum albumin and Hohenbuehelia serotina polysaccharides for the delivery of polyphenols isolated from the shells of Juglans regia L. (BSA-JRP-HSP NPs).
34554172	3	79	theme	%	729:729	arg1	±					723:723	88.47 ±	717:723	88.47 ± 0.04%	717:729	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	6	80	theme	propionic	1562:1570	arg1	acid					1572:1575	propionic acid	1562:1575	propionic acid	1562:1575	The production of short-chain fatty acids (SCFAs) including acetic acid, propionic acid, and butyric acid was remarkably enhanced by treatment with BSA-JRP-HSP NPs.
34554172	5	81	theme	microbiota	1255:1264	arg1	structure					1238:1246	structure	1238:1246	structure	1238:1246	After gastrointestinal digestion, BSA-JRP-HSP NPs could modulate the composition and structure of gut microbiota, promoting the growth of beneficial bacterial (e.g. Prevotella, Dialister, Akkermansia, etc.) and inhibiting the growth of pathogenic bacteria (e.g. Bacteroides, Phascolarctobacterium, Lachnospiracea incertae sedis, etc.).
34554172	5	81	theme	microbiota	1255:1264	arg1	composition					1222:1232	composition	1222:1232	composition	1222:1232	After gastrointestinal digestion, BSA-JRP-HSP NPs could modulate the composition and structure of gut microbiota, promoting the growth of beneficial bacterial (e.g. Prevotella, Dialister, Akkermansia, etc.) and inhibiting the growth of pathogenic bacteria (e.g. Bacteroides, Phascolarctobacterium, Lachnospiracea incertae sedis, etc.).
34554172	3	82	contain	have	677:680	arg1	NPs					600:602	BSA-JRP-HSP NPs	588:602	BSA-JRP-HSP NPs	588:602	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	3	82	contain	have	677:680	arg2	efficiency					703:712	a high encapsulation efficiency	682:712	a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV	682:810	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	3	83	theme	±	723:723	arg1	efficiency					703:712	a high encapsulation efficiency	682:712	a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV	682:810	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	3	84	theme	size	749:752	arg1	efficiency					703:712	a high encapsulation efficiency	682:712	a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV	682:810	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
34554172	3	85	theme	0.61	804:807	arg1	±					802:802	±	802:802	±	802:802	BSA-JRP-HSP NPs, with amorphous properties and regular spherical morphological features, have a high encapsulation efficiency of 88.47 ± 0.04%, average particle size of 285.7 ± 3.1 nm, and zeta potential of -12.20 ± 0.61 mV, and they exhibit excellent photothermal stabilities and strong mucin adhesion capacity.
33876290	4	0	theme	cross-linked	560:571	arg1	HA					573:574	cross-linked HA	560:574	cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method	560:704	OBJECTIVE In this study, we evaluated the properties, safety, and efficacy of a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method.
33876290	11	1	theme	HA-PN/PLLA	1620:1629	arg1	effect					1610:1615	the effect	1606:1615	the effect of HA-PN/PLLA in restoring skin structure	1606:1657	Furthermore, the results support the effect of HA-PN/PLLA in restoring skin structure.
33876290	13	2	theme	evidence	1944:1951	arg1	level					1935:1939	a level	1933:1939	a level of evidence	1933:1951	LEVEL OF EVIDENCE III This journal requires that authors assign a level of evidence to each article.
33876290	8	3	theme	composite	1193:1201	arg1	filler					1203:1208	HA-PN/PLLA composite filler	1182:1208	HA-PN/PLLA composite filler	1182:1208	HA-PN/PLLA composite filler had higher viscosity and elasticity values and similar osmolality as compared to those of HA and PN fillers.
33876290	12	4	theme	safe	1745:1748	arg1	filler					1727:1732	the novel composite filler	1707:1732	the novel composite filler	1707:1732	CONCLUSION Altogether, these data suggest that the novel composite filler might be a safe and effective option in terms of tissue integration, clinical management during delivery and high esthetic durability.
33876290	12	4	theme	safe	1745:1748	arg1	management					1812:1821	clinical management	1803:1821	clinical management during delivery and high esthetic durability	1803:1866	CONCLUSION Altogether, these data suggest that the novel composite filler might be a safe and effective option in terms of tissue integration, clinical management during delivery and high esthetic durability.
33876290	12	4	theme	safe	1745:1748	arg1	option					1764:1769	a safe and effective option	1743:1769	a safe and effective option in terms of tissue integration	1743:1800	CONCLUSION Altogether, these data suggest that the novel composite filler might be a safe and effective option in terms of tissue integration, clinical management during delivery and high esthetic durability.
33876290	8	5	contain	had	1210:1212	arg2	osmolality					1265:1274	similar osmolality	1257:1274	similar osmolality	1257:1274	HA-PN/PLLA composite filler had higher viscosity and elasticity values and similar osmolality as compared to those of HA and PN fillers.
33876290	8	5	contain	had	1210:1212	arg1	filler					1203:1208	HA-PN/PLLA composite filler	1182:1208	HA-PN/PLLA composite filler	1182:1208	HA-PN/PLLA composite filler had higher viscosity and elasticity values and similar osmolality as compared to those of HA and PN fillers.
33876290	8	5	contain	had	1210:1212	arg2	values					1246:1251	higher viscosity and elasticity values	1214:1251	higher viscosity and elasticity values	1214:1251	HA-PN/PLLA composite filler had higher viscosity and elasticity values and similar osmolality as compared to those of HA and PN fillers.
33876290	4	6	dep	OBJECTIVE	443:451	arg1	evaluated					471:479	evaluated	471:479	evaluated the properties, safety, and efficacy of a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method	471:704	OBJECTIVE In this study, we evaluated the properties, safety, and efficacy of a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method.
33876290	12	7	theme	effective	1754:1762	arg1	filler					1727:1732	the novel composite filler	1707:1732	the novel composite filler	1707:1732	CONCLUSION Altogether, these data suggest that the novel composite filler might be a safe and effective option in terms of tissue integration, clinical management during delivery and high esthetic durability.
33876290	12	7	theme	effective	1754:1762	arg1	management					1812:1821	clinical management	1803:1821	clinical management during delivery and high esthetic durability	1803:1866	CONCLUSION Altogether, these data suggest that the novel composite filler might be a safe and effective option in terms of tissue integration, clinical management during delivery and high esthetic durability.
33876290	12	7	theme	effective	1754:1762	arg1	option					1764:1769	a safe and effective option	1743:1769	a safe and effective option in terms of tissue integration	1743:1800	CONCLUSION Altogether, these data suggest that the novel composite filler might be a safe and effective option in terms of tissue integration, clinical management during delivery and high esthetic durability.
33876290	4	8	theme	Inventage	654:662	arg1	method					699:704	Inventage Lab Precision Particle Fabrication method	654:704	Inventage Lab Precision Particle Fabrication method	654:704	OBJECTIVE In this study, we evaluated the properties, safety, and efficacy of a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method.
33876290	10	9	theme	HWY/Slc	1550:1556	arg1	rats					1567:1570	HWY/Slc hairless rats	1550:1570	HWY/Slc hairless rats	1550:1570	In addition, the composite filler maintained a more stable volume than did HA filler for 24 weeks after administration in HWY/Slc hairless rats.
33876290	7	10	theme	particle	1129:1136	arg1	distribution					1143:1154	a narrower particle size distribution	1118:1154	a narrower particle size distribution	1118:1154	RESULTS Analysis of PLLA microspheres revealed spherical surfaces and a narrower particle size distribution than that in PLLA filler.
33876290	5	11	theme	electron	826:833	arg1	microscopy					835:844	scanning electron microscopy	817:844	scanning electron microscopy	817:844	MATERIALS AND METHODS The composition of the filler and characteristics of the microspheres were examined via scanning electron microscopy, particle size analysis, gel permeation chromatography, and rheology and osmolality measurement.
33876290	5	12	theme	permeation	875:884	arg1	chromatography					886:899	gel permeation chromatography	871:899	gel permeation chromatography	871:899	MATERIALS AND METHODS The composition of the filler and characteristics of the microspheres were examined via scanning electron microscopy, particle size analysis, gel permeation chromatography, and rheology and osmolality measurement.
33876290	8	13	theme	viscosity	1221:1229	arg1	values					1246:1251	higher viscosity and elasticity values	1214:1251	higher viscosity and elasticity values	1214:1251	HA-PN/PLLA composite filler had higher viscosity and elasticity values and similar osmolality as compared to those of HA and PN fillers.
33876290	4	14	theme	Precision	668:676	arg1	method					699:704	Inventage Lab Precision Particle Fabrication method	654:704	Inventage Lab Precision Particle Fabrication method	654:704	OBJECTIVE In this study, we evaluated the properties, safety, and efficacy of a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method.
33876290	8	15	theme	elasticity	1235:1244	arg1	values					1246:1251	higher viscosity and elasticity values	1214:1251	higher viscosity and elasticity values	1214:1251	HA-PN/PLLA composite filler had higher viscosity and elasticity values and similar osmolality as compared to those of HA and PN fillers.
33876290	6	16	theme	filler	1001:1006	arg1	efficacy					968:975	efficacy	968:975	efficacy	968:975	Additionally, safety and efficacy of HA-PN/PLLA composite filler were conducted in in vitro and in vivo.
33876290	6	16	theme	filler	1001:1006	arg1	safety					957:962	safety	957:962	safety	957:962	Additionally, safety and efficacy of HA-PN/PLLA composite filler were conducted in in vitro and in vivo.
33876290	13	17	dep	EVIDENCE	1878:1885	arg1	journal					1896:1902	This journal	1891:1902	EVIDENCE III This journal	1878:1902	LEVEL OF EVIDENCE III This journal requires that authors assign a level of evidence to each article.
33876290	4	18	theme	PLLA	617:620	arg1	microspheres					622:633	monodisperse PLLA microspheres	604:633	monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method	604:704	OBJECTIVE In this study, we evaluated the properties, safety, and efficacy of a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method.
33876290	5	19	theme	size	856:859	arg1	analysis					861:868	particle size analysis	847:868	particle size analysis	847:868	MATERIALS AND METHODS The composition of the filler and characteristics of the microspheres were examined via scanning electron microscopy, particle size analysis, gel permeation chromatography, and rheology and osmolality measurement.
33876290	6	20	theme	HA-PN/PLLA	980:989	arg1	filler					1001:1006	HA-PN/PLLA composite filler	980:1006	HA-PN/PLLA composite filler	980:1006	Additionally, safety and efficacy of HA-PN/PLLA composite filler were conducted in in vitro and in vivo.
33876290	8	21	theme	PN	1307:1308	arg1	fillers					1310:1316	PN fillers	1307:1316	PN fillers	1307:1316	HA-PN/PLLA composite filler had higher viscosity and elasticity values and similar osmolality as compared to those of HA and PN fillers.
33876290	4	22	with	combination	587:597	arg1	microspheres					622:633	monodisperse PLLA microspheres	604:633	monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method	604:704	OBJECTIVE In this study, we evaluated the properties, safety, and efficacy of a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method.
33876290	7	23	theme	PLLA	1068:1071	arg1	microspheres					1073:1084	PLLA microspheres	1068:1084	PLLA microspheres	1068:1084	RESULTS Analysis of PLLA microspheres revealed spherical surfaces and a narrower particle size distribution than that in PLLA filler.
33876290	2	24	theme	hyaluronic	227:236	arg1	HA					244:245	HA	244:245	HA	244:245	The natural polymer hyaluronic acid (HA) shows excellent biocompatibility and affects cell signaling, proliferation, and differentiation.
33876290	2	24	theme	hyaluronic	227:236	arg1	acid					238:241	The natural polymer hyaluronic acid	207:241	The natural polymer hyaluronic acid (HA)	207:246	The natural polymer hyaluronic acid (HA) shows excellent biocompatibility and affects cell signaling, proliferation, and differentiation.
33876290	1	25	theme	Poly-L-lactic	143:155	arg1	acid					157:160	BACKGROUND Poly-L-lactic acid	132:160	BACKGROUND Poly-L-lactic acid (PLLA)	132:167	BACKGROUND Poly-L-lactic acid (PLLA) is widely used in tissue engineering.
33876290	1	25	theme	Poly-L-lactic	143:155	arg1	PLLA					163:166	PLLA	163:166	PLLA	163:166	BACKGROUND Poly-L-lactic acid (PLLA) is widely used in tissue engineering.
33876290	4	26	with	HA	573:574	arg1	PN					581:582	PN	581:582	PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method	581:704	OBJECTIVE In this study, we evaluated the properties, safety, and efficacy of a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method.
33876290	12	27	theme	composite	1717:1725	arg1	filler					1727:1732	the novel composite filler	1707:1732	the novel composite filler	1707:1732	CONCLUSION Altogether, these data suggest that the novel composite filler might be a safe and effective option in terms of tissue integration, clinical management during delivery and high esthetic durability.
33876290	12	27	theme	composite	1717:1725	arg1	option					1764:1769	a safe and effective option	1743:1769	a safe and effective option in terms of tissue integration	1743:1800	CONCLUSION Altogether, these data suggest that the novel composite filler might be a safe and effective option in terms of tissue integration, clinical management during delivery and high esthetic durability.
33876290	4	28	theme	Fabrication	687:697	arg1	method					699:704	Inventage Lab Precision Particle Fabrication method	654:704	Inventage Lab Precision Particle Fabrication method	654:704	OBJECTIVE In this study, we evaluated the properties, safety, and efficacy of a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method.
33876290	5	29	theme	characteristics	763:777	arg1	composition					733:743	The composition	729:743	MATERIALS AND METHODS The composition of the filler and characteristics of the microspheres	707:797	MATERIALS AND METHODS The composition of the filler and characteristics of the microspheres were examined via scanning electron microscopy, particle size analysis, gel permeation chromatography, and rheology and osmolality measurement.
33876290	5	30	dep	MATERIALS	707:715	arg1	composition					733:743	The composition	729:743	MATERIALS AND METHODS The composition of the filler and characteristics of the microspheres	707:797	MATERIALS AND METHODS The composition of the filler and characteristics of the microspheres were examined via scanning electron microscopy, particle size analysis, gel permeation chromatography, and rheology and osmolality measurement.
33876290	10	31	theme	composite	1445:1453	arg1	filler					1455:1460	the composite filler	1441:1460	the composite filler	1441:1460	In addition, the composite filler maintained a more stable volume than did HA filler for 24 weeks after administration in HWY/Slc hairless rats.
33876290	13	32	theme	EVIDENCE	1878:1885	arg1	LEVEL					1869:1873	LEVEL	1869:1873	LEVEL OF EVIDENCE III This journal	1869:1902	LEVEL OF EVIDENCE III This journal requires that authors assign a level of evidence to each article.
33876290	0	33	theme	Acid-polynucleotide/Poly-L-lactic	68:100	arg1	Filler					124:129	a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler	49:129	a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler	49:129	Comparative Evaluation of Safety and Efficacy of a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler.
33876290	5	34	theme	osmolality	919:928	arg1	measurement					930:940	osmolality measurement	919:940	osmolality measurement	919:940	MATERIALS AND METHODS The composition of the filler and characteristics of the microspheres were examined via scanning electron microscopy, particle size analysis, gel permeation chromatography, and rheology and osmolality measurement.
33876290	2	35	theme	excellent	254:262	arg1	biocompatibility					264:279	excellent biocompatibility	254:279	excellent biocompatibility	254:279	The natural polymer hyaluronic acid (HA) shows excellent biocompatibility and affects cell signaling, proliferation, and differentiation.
33876290	14	36	theme	Instructions	2087:2098	arg1	Table					2055:2059	the Table	2051:2059	the Table of Contents or the online Instructions to Authors www.springer.com/00266	2051:2132	For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
33876290	0	37	theme	Composite	107:115	arg1	Filler					124:129	a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler	49:129	a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler	49:129	Comparative Evaluation of Safety and Efficacy of a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler.
33876290	2	38	theme	cell	293:296	arg1	signaling					298:306	cell signaling	293:306	cell signaling	293:306	The natural polymer hyaluronic acid (HA) shows excellent biocompatibility and affects cell signaling, proliferation, and differentiation.
33876290	3	39	theme	osteoblasts	430:440	arg1	growth					393:398	cell growth	388:398	cell growth of human skin fibroblasts and osteoblasts	388:440	In addition, a polynucleotide (PN) induces cell growth of human skin fibroblasts and osteoblasts.
33876290	0	40	theme	Filler	124:129	arg1	Efficacy					37:44	Efficacy	37:44	Efficacy	37:44	Comparative Evaluation of Safety and Efficacy of a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler.
33876290	0	40	theme	Filler	124:129	arg1	Safety					26:31	Safety	26:31	Safety	26:31	Comparative Evaluation of Safety and Efficacy of a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler.
33876290	0	41	theme	Efficacy	37:44	arg1	Evaluation					12:21	Comparative Evaluation	0:21	Comparative Evaluation of Safety and Efficacy of a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler.	0:130	Comparative Evaluation of Safety and Efficacy of a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler.
33876290	14	42	theme	Contents	2064:2071	arg1	Table					2055:2059	the Table	2051:2059	the Table of Contents or the online Instructions to Authors www.springer.com/00266	2051:2132	For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
33876290	3	43	theme	fibroblasts	414:424	arg1	growth					393:398	cell growth	388:398	cell growth of human skin fibroblasts and osteoblasts	388:440	In addition, a polynucleotide (PN) induces cell growth of human skin fibroblasts and osteoblasts.
33876290	0	44	theme	Comparative	0:10	arg1	Evaluation					12:21	Comparative Evaluation	0:21	Comparative Evaluation of Safety and Efficacy of a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler.	0:130	Comparative Evaluation of Safety and Efficacy of a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler.
33876290	9	45	theme	in	1351:1352	arg1	tests					1359:1363	in vitro and in vivo tests	1338:1363	in vitro and in vivo tests	1338:1363	The nontoxicity in in vitro and in vivo tests reflected that the composite filler may be safe for human use.
33876290	4	46	theme	novel	523:527	arg1	filler					539:544	a novel composite filler	521:544	a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method	521:704	OBJECTIVE In this study, we evaluated the properties, safety, and efficacy of a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method.
33876290	12	47	theme	esthetic	1848:1855	arg1	durability					1857:1866	high esthetic durability	1843:1866	high esthetic durability	1843:1866	CONCLUSION Altogether, these data suggest that the novel composite filler might be a safe and effective option in terms of tissue integration, clinical management during delivery and high esthetic durability.
33876290	3	48	theme	human	403:407	arg1	fibroblasts					414:424	human skin fibroblasts	403:424	human skin fibroblasts	403:424	In addition, a polynucleotide (PN) induces cell growth of human skin fibroblasts and osteoblasts.
33876290	4	49	theme	filler	539:544	arg1	safety					497:502	safety	497:502	safety	497:502	OBJECTIVE In this study, we evaluated the properties, safety, and efficacy of a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method.
33876290	4	49	theme	filler	539:544	arg1	efficacy					509:516	efficacy	509:516	efficacy	509:516	OBJECTIVE In this study, we evaluated the properties, safety, and efficacy of a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method.
33876290	4	49	theme	filler	539:544	arg1	properties					485:494	the properties	481:494	the properties	481:494	OBJECTIVE In this study, we evaluated the properties, safety, and efficacy of a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method.
33876290	0	50	theme	Novel	51:55	arg1	Filler					124:129	a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler	49:129	a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler	49:129	Comparative Evaluation of Safety and Efficacy of a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler.
33876290	7	51	theme	PLLA	1169:1172	arg1	filler					1174:1179	PLLA filler	1169:1179	PLLA filler	1169:1179	RESULTS Analysis of PLLA microspheres revealed spherical surfaces and a narrower particle size distribution than that in PLLA filler.
33876290	9	52	theme	composite	1384:1392	arg1	safe					1408:1411	safe	1408:1411	safe	1408:1411	The nontoxicity in in vitro and in vivo tests reflected that the composite filler may be safe for human use.
33876290	9	52	theme	composite	1384:1392	arg1	filler					1394:1399	the composite filler	1380:1399	the composite filler	1380:1399	The nontoxicity in in vitro and in vivo tests reflected that the composite filler may be safe for human use.
33876290	9	53	dep	in	1338:1339	arg1	vitro					1341:1345	vitro	1341:1345	vitro	1341:1345	The nontoxicity in in vitro and in vivo tests reflected that the composite filler may be safe for human use.
33876290	14	54	theme	ratings	2026:2032	arg1	description					1981:1991	a full description	1974:1991	a full description of these Evidence-Based Medicine ratings	1974:2032	For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
33876290	2	55	theme	natural	211:217	arg1	HA					244:245	HA	244:245	HA	244:245	The natural polymer hyaluronic acid (HA) shows excellent biocompatibility and affects cell signaling, proliferation, and differentiation.
33876290	2	55	theme	natural	211:217	arg1	acid					238:241	The natural polymer hyaluronic acid	207:241	The natural polymer hyaluronic acid (HA)	207:246	The natural polymer hyaluronic acid (HA) shows excellent biocompatibility and affects cell signaling, proliferation, and differentiation.
33876290	4	56	link	cross-linked	560:571	arg1	HA					573:574	cross-linked HA	560:574	cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method	560:704	OBJECTIVE In this study, we evaluated the properties, safety, and efficacy of a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method.
33876290	12	57	dep	CONCLUSION	1660:1669	arg1	suggest					1694:1700	suggest	1694:1700	suggest that the novel composite filler might be a safe and effective option in terms of tissue integration, clinical management during delivery and high esthetic durability	1694:1866	CONCLUSION Altogether, these data suggest that the novel composite filler might be a safe and effective option in terms of tissue integration, clinical management during delivery and high esthetic durability.
33876290	12	58	theme	tissue	1783:1788	arg1	integration					1790:1800	tissue integration	1783:1800	tissue integration	1783:1800	CONCLUSION Altogether, these data suggest that the novel composite filler might be a safe and effective option in terms of tissue integration, clinical management during delivery and high esthetic durability.
33876290	11	59	theme	skin	1644:1647	arg1	structure					1649:1657	skin structure	1644:1657	skin structure	1644:1657	Furthermore, the results support the effect of HA-PN/PLLA in restoring skin structure.
33876290	9	60	theme	human	1417:1421	arg1	use					1423:1425	human use	1417:1425	human use	1417:1425	The nontoxicity in in vitro and in vivo tests reflected that the composite filler may be safe for human use.
33876290	7	61	dep	RESULTS	1048:1054	arg1	revealed					1086:1093	revealed	1086:1093	revealed spherical surfaces and a narrower particle size distribution than that in PLLA filler	1086:1179	RESULTS Analysis of PLLA microspheres revealed spherical surfaces and a narrower particle size distribution than that in PLLA filler.
33876290	7	62	theme	size	1138:1141	arg1	distribution					1143:1154	a narrower particle size distribution	1118:1154	a narrower particle size distribution	1118:1154	RESULTS Analysis of PLLA microspheres revealed spherical surfaces and a narrower particle size distribution than that in PLLA filler.
33876290	8	63	theme	HA-PN/PLLA	1182:1191	arg1	filler					1203:1208	HA-PN/PLLA composite filler	1182:1208	HA-PN/PLLA composite filler	1182:1208	HA-PN/PLLA composite filler had higher viscosity and elasticity values and similar osmolality as compared to those of HA and PN fillers.
33876290	10	64	theme	hairless	1558:1565	arg1	rats					1567:1570	HWY/Slc hairless rats	1550:1570	HWY/Slc hairless rats	1550:1570	In addition, the composite filler maintained a more stable volume than did HA filler for 24 weeks after administration in HWY/Slc hairless rats.
33876290	7	65	theme	narrower	1120:1127	arg1	distribution					1143:1154	a narrower particle size distribution	1118:1154	a narrower particle size distribution	1118:1154	RESULTS Analysis of PLLA microspheres revealed spherical surfaces and a narrower particle size distribution than that in PLLA filler.
33876290	5	66	theme	gel	871:873	arg1	chromatography					886:899	gel permeation chromatography	871:899	gel permeation chromatography	871:899	MATERIALS AND METHODS The composition of the filler and characteristics of the microspheres were examined via scanning electron microscopy, particle size analysis, gel permeation chromatography, and rheology and osmolality measurement.
33876290	4	67	from	PN	581:582	arg1	combination					587:597	combination	587:597	combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method	587:704	OBJECTIVE In this study, we evaluated the properties, safety, and efficacy of a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method.
33876290	1	68	theme	tissue	187:192	arg1	engineering					194:204	tissue engineering	187:204	tissue engineering	187:204	BACKGROUND Poly-L-lactic acid (PLLA) is widely used in tissue engineering.
33876290	8	69	theme	higher	1214:1219	arg1	viscosity					1221:1229	higher viscosity	1214:1229	higher viscosity	1214:1229	HA-PN/PLLA composite filler had higher viscosity and elasticity values and similar osmolality as compared to those of HA and PN fillers.
33876290	4	70	theme	Lab	664:666	arg1	method					699:704	Inventage Lab Precision Particle Fabrication method	654:704	Inventage Lab Precision Particle Fabrication method	654:704	OBJECTIVE In this study, we evaluated the properties, safety, and efficacy of a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method.
33876290	7	71	theme	spherical	1095:1103	arg1	surfaces					1105:1112	spherical surfaces	1095:1112	spherical surfaces	1095:1112	RESULTS Analysis of PLLA microspheres revealed spherical surfaces and a narrower particle size distribution than that in PLLA filler.
33876290	5	72	theme	particle	847:854	arg1	analysis					861:868	particle size analysis	847:868	particle size analysis	847:868	MATERIALS AND METHODS The composition of the filler and characteristics of the microspheres were examined via scanning electron microscopy, particle size analysis, gel permeation chromatography, and rheology and osmolality measurement.
33876290	6	73	theme	composite	991:999	arg1	filler					1001:1006	HA-PN/PLLA composite filler	980:1006	HA-PN/PLLA composite filler	980:1006	Additionally, safety and efficacy of HA-PN/PLLA composite filler were conducted in in vitro and in vivo.
33876290	4	74	theme	monodisperse	604:615	arg1	microspheres					622:633	monodisperse PLLA microspheres	604:633	monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method	604:704	OBJECTIVE In this study, we evaluated the properties, safety, and efficacy of a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method.
33876290	7	75	theme	microspheres	1073:1084	arg1	Analysis					1056:1063	Analysis	1056:1063	Analysis of PLLA microspheres	1056:1084	RESULTS Analysis of PLLA microspheres revealed spherical surfaces and a narrower particle size distribution than that in PLLA filler.
33876290	9	76	from	nontoxicity	1323:1333	arg1	tests					1359:1363	in vitro and in vivo tests	1338:1363	in vitro and in vivo tests	1338:1363	The nontoxicity in in vitro and in vivo tests reflected that the composite filler may be safe for human use.
33876290	10	77	dep	volume	1487:1492	arg1	did					1499:1501	did	1499:1501	did	1499:1501	In addition, the composite filler maintained a more stable volume than did HA filler for 24 weeks after administration in HWY/Slc hairless rats.
33876290	1	78	theme	BACKGROUND	132:141	arg1	acid					157:160	BACKGROUND Poly-L-lactic acid	132:160	BACKGROUND Poly-L-lactic acid (PLLA)	132:167	BACKGROUND Poly-L-lactic acid (PLLA) is widely used in tissue engineering.
33876290	1	78	theme	BACKGROUND	132:141	arg1	PLLA					163:166	PLLA	163:166	PLLA	163:166	BACKGROUND Poly-L-lactic acid (PLLA) is widely used in tissue engineering.
33876290	8	79	theme	similar	1257:1263	arg1	osmolality					1265:1274	similar osmolality	1257:1274	similar osmolality	1257:1274	HA-PN/PLLA composite filler had higher viscosity and elasticity values and similar osmolality as compared to those of HA and PN fillers.
33876290	5	80	dep	filler	752:757	arg1	the					748:750	the	748:750	the	748:750	MATERIALS AND METHODS The composition of the filler and characteristics of the microspheres were examined via scanning electron microscopy, particle size analysis, gel permeation chromatography, and rheology and osmolality measurement.
33876290	0	81	theme	Hyaluronic	57:66	arg1	Filler					124:129	a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler	49:129	a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler	49:129	Comparative Evaluation of Safety and Efficacy of a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler.
33876290	12	82	theme	novel	1711:1715	arg1	filler					1727:1732	the novel composite filler	1707:1732	the novel composite filler	1707:1732	CONCLUSION Altogether, these data suggest that the novel composite filler might be a safe and effective option in terms of tissue integration, clinical management during delivery and high esthetic durability.
33876290	12	82	theme	novel	1711:1715	arg1	option					1764:1769	a safe and effective option	1743:1769	a safe and effective option in terms of tissue integration	1743:1800	CONCLUSION Altogether, these data suggest that the novel composite filler might be a safe and effective option in terms of tissue integration, clinical management during delivery and high esthetic durability.
33876290	1	83	used	used	179:182	arg2	PLLA					163:166	PLLA	163:166	PLLA	163:166	BACKGROUND Poly-L-lactic acid (PLLA) is widely used in tissue engineering.
33876290	1	83	used	used	179:182	arg2	acid					157:160	BACKGROUND Poly-L-lactic acid	132:160	BACKGROUND Poly-L-lactic acid (PLLA)	132:167	BACKGROUND Poly-L-lactic acid (PLLA) is widely used in tissue engineering.
33876290	4	84	theme	Particle	678:685	arg1	method					699:704	Inventage Lab Precision Particle Fabrication method	654:704	Inventage Lab Precision Particle Fabrication method	654:704	OBJECTIVE In this study, we evaluated the properties, safety, and efficacy of a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method.
33876290	0	85	theme	Acid	102:105	arg1	Filler					124:129	a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler	49:129	a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler	49:129	Comparative Evaluation of Safety and Efficacy of a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler.
33876290	14	86	theme	Medicine	2017:2024	arg1	ratings					2026:2032	these Evidence-Based Medicine ratings	1996:2032	these Evidence-Based Medicine ratings	1996:2032	For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
33876290	9	87	dep	in	1351:1352	arg1	vivo					1354:1357	vivo	1354:1357	vivo	1354:1357	The nontoxicity in in vitro and in vivo tests reflected that the composite filler may be safe for human use.
33876290	0	88	theme	Dermal	117:122	arg1	Filler					124:129	a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler	49:129	a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler	49:129	Comparative Evaluation of Safety and Efficacy of a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler.
33876290	14	89	theme	Evidence-Based	2002:2015	arg1	ratings					2026:2032	these Evidence-Based Medicine ratings	1996:2032	these Evidence-Based Medicine ratings	1996:2032	For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
33876290	14	90	theme	online	2080:2085	arg1	Instructions					2087:2098	the online Instructions	2076:2098	the online Instructions to Authors www.springer.com/00266	2076:2132	For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
33876290	10	91	dep	did	1499:1501	arg1	than					1494:1497	than	1494:1497	than	1494:1497	In addition, the composite filler maintained a more stable volume than did HA filler for 24 weeks after administration in HWY/Slc hairless rats.
33876290	9	92	theme	in	1338:1339	arg1	tests					1359:1363	in vitro and in vivo tests	1338:1363	in vitro and in vivo tests	1338:1363	The nontoxicity in in vitro and in vivo tests reflected that the composite filler may be safe for human use.
33876290	12	93	from	option	1764:1769	arg1	terms					1774:1778	terms	1774:1778	terms of tissue integration	1774:1800	CONCLUSION Altogether, these data suggest that the novel composite filler might be a safe and effective option in terms of tissue integration, clinical management during delivery and high esthetic durability.
33876290	5	94	theme	scanning	817:824	arg1	microscopy					835:844	scanning electron microscopy	817:844	scanning electron microscopy	817:844	MATERIALS AND METHODS The composition of the filler and characteristics of the microspheres were examined via scanning electron microscopy, particle size analysis, gel permeation chromatography, and rheology and osmolality measurement.
33876290	14	95	theme	full	1976:1979	arg1	description					1981:1991	a full description	1974:1991	a full description of these Evidence-Based Medicine ratings	1974:2032	For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
33876290	3	96	theme	cell	388:391	arg1	growth					393:398	cell growth	388:398	cell growth of human skin fibroblasts and osteoblasts	388:440	In addition, a polynucleotide (PN) induces cell growth of human skin fibroblasts and osteoblasts.
33876290	4	97	theme	composite	529:537	arg1	filler					539:544	a novel composite filler	521:544	a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method	521:704	OBJECTIVE In this study, we evaluated the properties, safety, and efficacy of a novel composite filler consisting of cross-linked HA with PN in combination with monodisperse PLLA microspheres manufactured using Inventage Lab Precision Particle Fabrication method.
33876290	0	98	theme	Safety	26:31	arg1	Evaluation					12:21	Comparative Evaluation	0:21	Comparative Evaluation of Safety and Efficacy of a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler.	0:130	Comparative Evaluation of Safety and Efficacy of a Novel Hyaluronic Acid-polynucleotide/Poly-L-lactic Acid Composite Dermal Filler.
33876290	5	99	theme	microspheres	786:797	arg1	characteristics					763:777	characteristics	763:777	characteristics	763:777	MATERIALS AND METHODS The composition of the filler and characteristics of the microspheres were examined via scanning electron microscopy, particle size analysis, gel permeation chromatography, and rheology and osmolality measurement.
33876290	5	99	theme	microspheres	786:797	arg1	filler					752:757	filler	752:757	filler	752:757	MATERIALS AND METHODS The composition of the filler and characteristics of the microspheres were examined via scanning electron microscopy, particle size analysis, gel permeation chromatography, and rheology and osmolality measurement.
33876290	3	100	theme	skin	409:412	arg1	fibroblasts					414:424	human skin fibroblasts	403:424	human skin fibroblasts	403:424	In addition, a polynucleotide (PN) induces cell growth of human skin fibroblasts and osteoblasts.
33876290	10	101	theme	stable	1480:1485	arg1	volume					1487:1492	a more stable volume	1473:1492	a more stable volume than did HA filler for 24 weeks after administration in HWY/Slc hairless rats	1473:1570	In addition, the composite filler maintained a more stable volume than did HA filler for 24 weeks after administration in HWY/Slc hairless rats.
33876290	5	102	theme	filler	752:757	arg1	composition					733:743	The composition	729:743	MATERIALS AND METHODS The composition of the filler and characteristics of the microspheres	707:797	MATERIALS AND METHODS The composition of the filler and characteristics of the microspheres were examined via scanning electron microscopy, particle size analysis, gel permeation chromatography, and rheology and osmolality measurement.
33876290	10	103	from	administration	1532:1545	arg1	rats					1567:1570	HWY/Slc hairless rats	1550:1570	HWY/Slc hairless rats	1550:1570	In addition, the composite filler maintained a more stable volume than did HA filler for 24 weeks after administration in HWY/Slc hairless rats.
33876290	12	104	theme	integration	1790:1800	arg1	terms					1774:1778	terms	1774:1778	terms of tissue integration	1774:1800	CONCLUSION Altogether, these data suggest that the novel composite filler might be a safe and effective option in terms of tissue integration, clinical management during delivery and high esthetic durability.
33876290	2	105	theme	polymer	219:225	arg1	HA					244:245	HA	244:245	HA	244:245	The natural polymer hyaluronic acid (HA) shows excellent biocompatibility and affects cell signaling, proliferation, and differentiation.
33876290	2	105	theme	polymer	219:225	arg1	acid					238:241	The natural polymer hyaluronic acid	207:241	The natural polymer hyaluronic acid (HA)	207:246	The natural polymer hyaluronic acid (HA) shows excellent biocompatibility and affects cell signaling, proliferation, and differentiation.
33876290	12	106	theme	high	1843:1846	arg1	durability					1857:1866	high esthetic durability	1843:1866	high esthetic durability	1843:1866	CONCLUSION Altogether, these data suggest that the novel composite filler might be a safe and effective option in terms of tissue integration, clinical management during delivery and high esthetic durability.
33876290	12	107	theme	clinical	1803:1810	arg1	option					1764:1769	a safe and effective option	1743:1769	a safe and effective option in terms of tissue integration	1743:1800	CONCLUSION Altogether, these data suggest that the novel composite filler might be a safe and effective option in terms of tissue integration, clinical management during delivery and high esthetic durability.
33876290	12	107	theme	clinical	1803:1810	arg1	management					1812:1821	clinical management	1803:1821	clinical management during delivery and high esthetic durability	1803:1866	CONCLUSION Altogether, these data suggest that the novel composite filler might be a safe and effective option in terms of tissue integration, clinical management during delivery and high esthetic durability.
33876290	10	108	theme	HA	1503:1504	arg1	filler					1506:1511	HA filler	1503:1511	HA filler for 24 weeks after administration in HWY/Slc hairless rats	1503:1570	In addition, the composite filler maintained a more stable volume than did HA filler for 24 weeks after administration in HWY/Slc hairless rats.
34980391	0	0	theme	γ-irradiated	85:96	arg1	commune					131:137	γ-irradiated and non-irradiated Schizophyllum commune	85:137	γ-irradiated and non-irradiated Schizophyllum commune	85:137	Differences of gut microbiota composition in mice supplied with polysaccharides from γ-irradiated and non-irradiated Schizophyllum commune.
34980391	2	1	theme	weight	350:355	arg1	gain					357:360	body weight gain	345:360	body weight gain	345:360	The results showed that N-SFP and I-SFP supplementation prevent body weight gain, enhance kidney uric acid metabolism and increase the concentration of SCFAs to a certain extent.
34980391	1	2	theme	normal	176:181	arg1	commune					230:236	normal (N-SFP) and γ-irradiated (I-SFP) Schizophyllum commune	176:236	normal (N-SFP) and γ-irradiated (I-SFP) Schizophyllum commune	176:236	In this study, polysaccharides from normal (N-SFP) and γ-irradiated (I-SFP) Schizophyllum commune were supplied to Kunming mice for 30 days.
34980391	1	3	theme	Kunming	255:261	arg1	mice					263:266	Kunming mice	255:266	Kunming mice	255:266	In this study, polysaccharides from normal (N-SFP) and γ-irradiated (I-SFP) Schizophyllum commune were supplied to Kunming mice for 30 days.
34980391	0	4	theme	non-irradiated	102:115	arg1	commune					131:137	γ-irradiated and non-irradiated Schizophyllum commune	85:137	γ-irradiated and non-irradiated Schizophyllum commune	85:137	Differences of gut microbiota composition in mice supplied with polysaccharides from γ-irradiated and non-irradiated Schizophyllum commune.
34980391	0	5	from	Differences	0:10	arg1	mice					45:48	mice	45:48	mice	45:48	Differences of gut microbiota composition in mice supplied with polysaccharides from γ-irradiated and non-irradiated Schizophyllum commune.
34980391	3	6	theme	gut	519:521	arg1	microbiota					523:532	beneficial gut microbiota	508:532	beneficial gut microbiota	508:532	Moreover, N-SFP and I-SFP promote the growth of beneficial gut microbiota and inhibit the growth of harmful bacteria.
34980391	5	7	theme	health	966:971	arg1	supplement					973:982	a potential health supplement	954:982	a potential health supplement in the prevention of obesity	954:1011	In total, I-SFP showed better effects than N-SFP in preventing weight gain, and modulating the mice gut microbiota, which suggests that I-SFP could act as a potential health supplement in the prevention of obesity.
34980391	5	7	theme	health	966:971	arg1	I-SFP					935:939	I-SFP	935:939	I-SFP	935:939	In total, I-SFP showed better effects than N-SFP in preventing weight gain, and modulating the mice gut microbiota, which suggests that I-SFP could act as a potential health supplement in the prevention of obesity.
34980391	3	8	theme	microbiota	523:532	arg1	growth					498:503	the growth	494:503	the growth of beneficial gut microbiota	494:532	Moreover, N-SFP and I-SFP promote the growth of beneficial gut microbiota and inhibit the growth of harmful bacteria.
34980391	5	9	from	supplement	973:982	arg1	prevention					991:1000	the prevention	987:1000	the prevention of obesity	987:1011	In total, I-SFP showed better effects than N-SFP in preventing weight gain, and modulating the mice gut microbiota, which suggests that I-SFP could act as a potential health supplement in the prevention of obesity.
34980391	5	10	theme	mice	894:897	arg1	microbiota					903:912	the mice gut microbiota	890:912	the mice gut microbiota	890:912	In total, I-SFP showed better effects than N-SFP in preventing weight gain, and modulating the mice gut microbiota, which suggests that I-SFP could act as a potential health supplement in the prevention of obesity.
34980391	4	11	theme	Lachnospiraceae	763:777	arg1	family					736:741	the family	732:741	the family of Akkermansiaceae, Lachnospiraceae and Bacteroidaceae	732:796	Compared to N-SFP, I-SFP decreased the relative abundance of Muribaculaceae and Lactobacillaceae, and increased the beneficial gut microbiota, especially the family of Akkermansiaceae, Lachnospiraceae and Bacteroidaceae.
34980391	4	11	theme	Lachnospiraceae	763:777	arg1	microbiota					709:718	the beneficial gut microbiota	690:718	the beneficial gut microbiota	690:718	Compared to N-SFP, I-SFP decreased the relative abundance of Muribaculaceae and Lactobacillaceae, and increased the beneficial gut microbiota, especially the family of Akkermansiaceae, Lachnospiraceae and Bacteroidaceae.
34980391	5	12	theme	weight	862:867	arg1	gain					869:872	weight gain	862:872	weight gain	862:872	In total, I-SFP showed better effects than N-SFP in preventing weight gain, and modulating the mice gut microbiota, which suggests that I-SFP could act as a potential health supplement in the prevention of obesity.
34980391	2	13	theme	acid	383:386	arg1	metabolism					388:397	kidney uric acid metabolism	371:397	kidney uric acid metabolism	371:397	The results showed that N-SFP and I-SFP supplementation prevent body weight gain, enhance kidney uric acid metabolism and increase the concentration of SCFAs to a certain extent.
34980391	0	14	theme	Schizophyllum	117:129	arg1	commune					131:137	γ-irradiated and non-irradiated Schizophyllum commune	85:137	γ-irradiated and non-irradiated Schizophyllum commune	85:137	Differences of gut microbiota composition in mice supplied with polysaccharides from γ-irradiated and non-irradiated Schizophyllum commune.
34980391	3	15	theme	beneficial	508:517	arg1	microbiota					523:532	beneficial gut microbiota	508:532	beneficial gut microbiota	508:532	Moreover, N-SFP and I-SFP promote the growth of beneficial gut microbiota and inhibit the growth of harmful bacteria.
34980391	2	16	theme	uric	378:381	arg1	acid					383:386	kidney uric acid	371:386	kidney uric acid metabolism	371:397	The results showed that N-SFP and I-SFP supplementation prevent body weight gain, enhance kidney uric acid metabolism and increase the concentration of SCFAs to a certain extent.
34980391	2	17	theme	kidney	371:376	arg1	acid					383:386	kidney uric acid	371:386	kidney uric acid metabolism	371:397	The results showed that N-SFP and I-SFP supplementation prevent body weight gain, enhance kidney uric acid metabolism and increase the concentration of SCFAs to a certain extent.
34980391	2	18	theme	SCFAs	433:437	arg1	concentration					416:428	the concentration	412:428	the concentration of SCFAs to a certain extent	412:457	The results showed that N-SFP and I-SFP supplementation prevent body weight gain, enhance kidney uric acid metabolism and increase the concentration of SCFAs to a certain extent.
34980391	1	19	dep	normal	176:181	arg1	N-SFP					184:188	N-SFP	184:188	N-SFP	184:188	In this study, polysaccharides from normal (N-SFP) and γ-irradiated (I-SFP) Schizophyllum commune were supplied to Kunming mice for 30 days.
34980391	4	20	theme	Muribaculaceae	639:652	arg1	abundance					626:634	the relative abundance	613:634	the relative abundance of Muribaculaceae and Lactobacillaceae	613:673	Compared to N-SFP, I-SFP decreased the relative abundance of Muribaculaceae and Lactobacillaceae, and increased the beneficial gut microbiota, especially the family of Akkermansiaceae, Lachnospiraceae and Bacteroidaceae.
34980391	0	21	theme	microbiota	19:28	arg1	composition					30:40	gut microbiota composition	15:40	gut microbiota composition	15:40	Differences of gut microbiota composition in mice supplied with polysaccharides from γ-irradiated and non-irradiated Schizophyllum commune.
34980391	5	22	theme	obesity	1005:1011	arg1	prevention					991:1000	the prevention	987:1000	the prevention of obesity	987:1011	In total, I-SFP showed better effects than N-SFP in preventing weight gain, and modulating the mice gut microbiota, which suggests that I-SFP could act as a potential health supplement in the prevention of obesity.
34980391	1	23	theme	γ-irradiated	195:206	arg1	commune					230:236	normal (N-SFP) and γ-irradiated (I-SFP) Schizophyllum commune	176:236	normal (N-SFP) and γ-irradiated (I-SFP) Schizophyllum commune	176:236	In this study, polysaccharides from normal (N-SFP) and γ-irradiated (I-SFP) Schizophyllum commune were supplied to Kunming mice for 30 days.
34980391	0	24	theme	gut	15:17	arg1	composition					30:40	gut microbiota composition	15:40	gut microbiota composition	15:40	Differences of gut microbiota composition in mice supplied with polysaccharides from γ-irradiated and non-irradiated Schizophyllum commune.
34980391	2	25	theme	body	345:348	arg1	gain					357:360	body weight gain	345:360	body weight gain	345:360	The results showed that N-SFP and I-SFP supplementation prevent body weight gain, enhance kidney uric acid metabolism and increase the concentration of SCFAs to a certain extent.
34980391	3	26	theme	bacteria	568:575	arg1	growth					550:555	the growth	546:555	the growth of harmful bacteria	546:575	Moreover, N-SFP and I-SFP promote the growth of beneficial gut microbiota and inhibit the growth of harmful bacteria.
34980391	4	27	theme	relative	617:624	arg1	abundance					626:634	the relative abundance	613:634	the relative abundance of Muribaculaceae and Lactobacillaceae	613:673	Compared to N-SFP, I-SFP decreased the relative abundance of Muribaculaceae and Lactobacillaceae, and increased the beneficial gut microbiota, especially the family of Akkermansiaceae, Lachnospiraceae and Bacteroidaceae.
34980391	0	28	theme	composition	30:40	arg1	Differences					0:10	Differences	0:10	Differences of gut microbiota composition in mice	0:48	Differences of gut microbiota composition in mice supplied with polysaccharides from γ-irradiated and non-irradiated Schizophyllum commune.
34980391	2	29	theme	certain	444:450	arg1	extent					452:457	a certain extent	442:457	a certain extent	442:457	The results showed that N-SFP and I-SFP supplementation prevent body weight gain, enhance kidney uric acid metabolism and increase the concentration of SCFAs to a certain extent.
34980391	4	30	theme	beneficial	694:703	arg1	family					736:741	the family	732:741	the family of Akkermansiaceae, Lachnospiraceae and Bacteroidaceae	732:796	Compared to N-SFP, I-SFP decreased the relative abundance of Muribaculaceae and Lactobacillaceae, and increased the beneficial gut microbiota, especially the family of Akkermansiaceae, Lachnospiraceae and Bacteroidaceae.
34980391	4	30	theme	beneficial	694:703	arg1	microbiota					709:718	the beneficial gut microbiota	690:718	the beneficial gut microbiota	690:718	Compared to N-SFP, I-SFP decreased the relative abundance of Muribaculaceae and Lactobacillaceae, and increased the beneficial gut microbiota, especially the family of Akkermansiaceae, Lachnospiraceae and Bacteroidaceae.
34980391	0	31	from	commune	131:137	arg1	polysaccharides					64:78	polysaccharides	64:78	polysaccharides from γ-irradiated and non-irradiated Schizophyllum commune	64:137	Differences of gut microbiota composition in mice supplied with polysaccharides from γ-irradiated and non-irradiated Schizophyllum commune.
34980391	3	32	theme	harmful	560:566	arg1	bacteria					568:575	harmful bacteria	560:575	harmful bacteria	560:575	Moreover, N-SFP and I-SFP promote the growth of beneficial gut microbiota and inhibit the growth of harmful bacteria.
34980391	5	33	theme	gut	899:901	arg1	microbiota					903:912	the mice gut microbiota	890:912	the mice gut microbiota	890:912	In total, I-SFP showed better effects than N-SFP in preventing weight gain, and modulating the mice gut microbiota, which suggests that I-SFP could act as a potential health supplement in the prevention of obesity.
34980391	5	34	theme	potential	956:964	arg1	supplement					973:982	a potential health supplement	954:982	a potential health supplement in the prevention of obesity	954:1011	In total, I-SFP showed better effects than N-SFP in preventing weight gain, and modulating the mice gut microbiota, which suggests that I-SFP could act as a potential health supplement in the prevention of obesity.
34980391	5	34	theme	potential	956:964	arg1	I-SFP					935:939	I-SFP	935:939	I-SFP	935:939	In total, I-SFP showed better effects than N-SFP in preventing weight gain, and modulating the mice gut microbiota, which suggests that I-SFP could act as a potential health supplement in the prevention of obesity.
34980391	4	35	theme	Bacteroidaceae	783:796	arg1	family					736:741	the family	732:741	the family of Akkermansiaceae, Lachnospiraceae and Bacteroidaceae	732:796	Compared to N-SFP, I-SFP decreased the relative abundance of Muribaculaceae and Lactobacillaceae, and increased the beneficial gut microbiota, especially the family of Akkermansiaceae, Lachnospiraceae and Bacteroidaceae.
34980391	4	35	theme	Bacteroidaceae	783:796	arg1	microbiota					709:718	the beneficial gut microbiota	690:718	the beneficial gut microbiota	690:718	Compared to N-SFP, I-SFP decreased the relative abundance of Muribaculaceae and Lactobacillaceae, and increased the beneficial gut microbiota, especially the family of Akkermansiaceae, Lachnospiraceae and Bacteroidaceae.
34980391	2	36	theme	I-SFP	315:319	arg1	supplementation					321:335	I-SFP supplementation	315:335	I-SFP supplementation	315:335	The results showed that N-SFP and I-SFP supplementation prevent body weight gain, enhance kidney uric acid metabolism and increase the concentration of SCFAs to a certain extent.
34980391	1	37	theme	Schizophyllum	216:228	arg1	commune					230:236	normal (N-SFP) and γ-irradiated (I-SFP) Schizophyllum commune	176:236	normal (N-SFP) and γ-irradiated (I-SFP) Schizophyllum commune	176:236	In this study, polysaccharides from normal (N-SFP) and γ-irradiated (I-SFP) Schizophyllum commune were supplied to Kunming mice for 30 days.
34980391	1	38	from	commune	230:236	arg1	polysaccharides					155:169	polysaccharides	155:169	polysaccharides from normal (N-SFP) and γ-irradiated (I-SFP) Schizophyllum commune	155:236	In this study, polysaccharides from normal (N-SFP) and γ-irradiated (I-SFP) Schizophyllum commune were supplied to Kunming mice for 30 days.
34980391	4	39	theme	Akkermansiaceae	746:760	arg1	family					736:741	the family	732:741	the family of Akkermansiaceae, Lachnospiraceae and Bacteroidaceae	732:796	Compared to N-SFP, I-SFP decreased the relative abundance of Muribaculaceae and Lactobacillaceae, and increased the beneficial gut microbiota, especially the family of Akkermansiaceae, Lachnospiraceae and Bacteroidaceae.
34980391	4	39	theme	Akkermansiaceae	746:760	arg1	microbiota					709:718	the beneficial gut microbiota	690:718	the beneficial gut microbiota	690:718	Compared to N-SFP, I-SFP decreased the relative abundance of Muribaculaceae and Lactobacillaceae, and increased the beneficial gut microbiota, especially the family of Akkermansiaceae, Lachnospiraceae and Bacteroidaceae.
34980391	4	40	theme	gut	705:707	arg1	family					736:741	the family	732:741	the family of Akkermansiaceae, Lachnospiraceae and Bacteroidaceae	732:796	Compared to N-SFP, I-SFP decreased the relative abundance of Muribaculaceae and Lactobacillaceae, and increased the beneficial gut microbiota, especially the family of Akkermansiaceae, Lachnospiraceae and Bacteroidaceae.
34980391	4	40	theme	gut	705:707	arg1	microbiota					709:718	the beneficial gut microbiota	690:718	the beneficial gut microbiota	690:718	Compared to N-SFP, I-SFP decreased the relative abundance of Muribaculaceae and Lactobacillaceae, and increased the beneficial gut microbiota, especially the family of Akkermansiaceae, Lachnospiraceae and Bacteroidaceae.
34980391	1	41	dep	γ-irradiated	195:206	arg1	I-SFP					209:213	I-SFP	209:213	I-SFP	209:213	In this study, polysaccharides from normal (N-SFP) and γ-irradiated (I-SFP) Schizophyllum commune were supplied to Kunming mice for 30 days.
34980391	5	42	theme	better	822:827	arg1	effects					829:835	better effects	822:835	better effects	822:835	In total, I-SFP showed better effects than N-SFP in preventing weight gain, and modulating the mice gut microbiota, which suggests that I-SFP could act as a potential health supplement in the prevention of obesity.
34980391	4	43	theme	Lactobacillaceae	658:673	arg1	abundance					626:634	the relative abundance	613:634	the relative abundance of Muribaculaceae and Lactobacillaceae	613:673	Compared to N-SFP, I-SFP decreased the relative abundance of Muribaculaceae and Lactobacillaceae, and increased the beneficial gut microbiota, especially the family of Akkermansiaceae, Lachnospiraceae and Bacteroidaceae.
32033042	8	0	from	coffee	1388:1393	arg1	amount					1353:1358	a considerable amount	1338:1358	a considerable amount of the carbohydrates in the coffee	1338:1393	The enzymatic extract proved to be very stable for 24 h, at 50 °C, and was able to hydrolyze a considerable amount of the carbohydrates in the coffee.
32033042	8	0	from	coffee	1388:1393	arg1	carbohydrates					1367:1379	the carbohydrates	1363:1379	the carbohydrates in the coffee	1363:1393	The enzymatic extract proved to be very stable for 24 h, at 50 °C, and was able to hydrolyze a considerable amount of the carbohydrates in the coffee.
32033042	3	1	theme	coffee	711:716	arg1	polysaccharides					718:732	waste coffee polysaccharides	705:732	waste coffee polysaccharides from soluble coffee processing	705:763	This work describes the production of hydrolytic enzymes by solid-state fermentation (SSF), cultivating filamentous fungi on waste from the coffee industry, followed by their application in the hydrolysis of waste coffee polysaccharides from soluble coffee processing.
32033042	1	2	theme	value	155:159	arg1	benefits					132:139	the combined benefits	119:139	the combined benefits of high added value and practicality for its consumers	119:194	Soluble coffee offers the combined benefits of high added value and practicality for its consumers.
32033042	0	3	theme	Coffee	78:83	arg1	Processing					85:94	Soluble Coffee Processing	70:94	Soluble Coffee Processing	70:94	β-Mannanase Production Using Coffee Industry Waste for Application in Soluble Coffee Processing.
32033042	7	4	theme	best	1090:1093	arg1	results					1118:1124	The best β-mannanase production results	1086:1124	The best β-mannanase production results	1086:1124	The best β-mannanase production results were found for a substrate composed of a mixture of coffee waste and wheat bran (1:1 w/w), using Aspergillusniger F12.
32033042	7	5	theme	Aspergillusniger	1223:1238	arg1	F12					1240:1242	Aspergillusniger F12	1223:1242	Aspergillusniger F12	1223:1242	The best β-mannanase production results were found for a substrate composed of a mixture of coffee waste and wheat bran (1:1 w/w), using Aspergillusniger F12.
32033042	7	6	theme	bran	1201:1204	arg1	mixture					1167:1173	a mixture	1165:1173	a mixture of coffee waste and wheat bran (1:1 w/w)	1165:1214	The best β-mannanase production results were found for a substrate composed of a mixture of coffee waste and wheat bran (1:1 w/w), using Aspergillusniger F12.
32033042	10	7	theme	circular	1757:1764	arg1	economy					1766:1772	a circular economy	1755:1772	a circular economy	1755:1772	The production of β-mannanase by SSF and its application in the hydrolysis of coffee polysaccharides showed promise for improving soluble coffee processing, offering an attractive way to assist in closing the loops in the coffee industry and creating a circular economy.
32033042	2	8	contain	have	428:431	arg2	requirements					446:457	lower energy requirements	433:457	lower energy requirements	433:457	The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes, is an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts.
32033042	2	8	contain	have	428:431	arg1	processes					413:421	industrial processes	402:421	industrial processes that have lower energy requirements and can reduce environmental impacts	402:494	The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes, is an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts.
32033042	3	9	theme	enzymes	546:552	arg1	production					521:530	the production	517:530	the production of hydrolytic enzymes by solid-state fermentation (SSF)	517:586	This work describes the production of hydrolytic enzymes by solid-state fermentation (SSF), cultivating filamentous fungi on waste from the coffee industry, followed by their application in the hydrolysis of waste coffee polysaccharides from soluble coffee processing.
32033042	5	10	theme	packed-bed	970:979	arg1	bioreactor					981:990	a packed-bed bioreactor	968:990	a packed-bed bioreactor	968:990	Cultivations for enzymes production were carried out in flasks and in a packed-bed bioreactor.
32033042	10	11	theme	coffee	1642:1647	arg1	processing					1649:1658	soluble coffee processing	1634:1658	soluble coffee processing	1634:1658	The production of β-mannanase by SSF and its application in the hydrolysis of coffee polysaccharides showed promise for improving soluble coffee processing, offering an attractive way to assist in closing the loops in the coffee industry and creating a circular economy.
32033042	3	12	theme	polysaccharides	718:732	arg1	hydrolysis					691:700	the hydrolysis	687:700	the hydrolysis of waste coffee polysaccharides from soluble coffee processing	687:763	This work describes the production of hydrolytic enzymes by solid-state fermentation (SSF), cultivating filamentous fungi on waste from the coffee industry, followed by their application in the hydrolysis of waste coffee polysaccharides from soluble coffee processing.
32033042	4	13	theme	Different	766:774	arg1	compositions					786:797	Different substrate compositions	766:797	Different substrate compositions	766:797	Different substrate compositions were studied, an ideal microorganism was selected, and the fermentation conditions were optimized.
32033042	8	14	from	amount	1353:1358	arg1	coffee					1388:1393	the coffee	1384:1393	the coffee	1384:1393	The enzymatic extract proved to be very stable for 24 h, at 50 °C, and was able to hydrolyze a considerable amount of the carbohydrates in the coffee.
32033042	3	15	from	industry	644:651	arg1	waste					622:626	waste	622:626	waste from the coffee industry, followed by their application in the hydrolysis of waste coffee polysaccharides from soluble coffee processing	622:763	This work describes the production of hydrolytic enzymes by solid-state fermentation (SSF), cultivating filamentous fungi on waste from the coffee industry, followed by their application in the hydrolysis of waste coffee polysaccharides from soluble coffee processing.
32033042	7	16	theme	production	1107:1116	arg1	results					1118:1124	The best β-mannanase production results	1086:1124	The best β-mannanase production results	1086:1124	The best β-mannanase production results were found for a substrate composed of a mixture of coffee waste and wheat bran (1:1 w/w), using Aspergillusniger F12.
32033042	10	17	from	application	1549:1559	arg1	hydrolysis					1568:1577	the hydrolysis	1564:1577	the hydrolysis of coffee polysaccharides	1564:1603	The production of β-mannanase by SSF and its application in the hydrolysis of coffee polysaccharides showed promise for improving soluble coffee processing, offering an attractive way to assist in closing the loops in the coffee industry and creating a circular economy.
32033042	1	18	theme	practicality	165:176	arg1	benefits					132:139	the combined benefits	119:139	the combined benefits of high added value and practicality for its consumers	119:194	Soluble coffee offers the combined benefits of high added value and practicality for its consumers.
32033042	7	19	theme	wheat	1195:1199	arg1	bran					1201:1204	wheat bran	1195:1204	wheat bran (1:1 w/w)	1195:1214	The best β-mannanase production results were found for a substrate composed of a mixture of coffee waste and wheat bran (1:1 w/w), using Aspergillusniger F12.
32033042	7	19	theme	wheat	1195:1199	arg1	w/w					1211:1213	1:1 w/w	1207:1213	1:1 w/w	1207:1213	The best β-mannanase production results were found for a substrate composed of a mixture of coffee waste and wheat bran (1:1 w/w), using Aspergillusniger F12.
32033042	0	20	from	Waste	45:49	arg1	Processing					85:94	Soluble Coffee Processing	70:94	Soluble Coffee Processing	70:94	β-Mannanase Production Using Coffee Industry Waste for Application in Soluble Coffee Processing.
32033042	9	21	theme	crude	1451:1455	arg1	extract					1457:1463	the crude extract	1447:1463	the crude extract	1447:1463	The addition of a commercial cellulase cocktail to the crude extract increased the hydrolysis yield by 56%.
32033042	2	22	theme	lower	433:437	arg1	requirements					446:457	lower energy requirements	433:457	lower energy requirements	433:457	The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes, is an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts.
32033042	2	23	theme	energy	439:444	arg1	requirements					446:457	lower energy requirements	433:457	lower energy requirements	433:457	The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes, is an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts.
32033042	2	24	theme	environmental	474:486	arg1	impacts					488:494	environmental impacts	474:494	environmental impacts	474:494	The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes, is an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts.
32033042	3	25	from	processing	754:763	arg1	hydrolysis					691:700	the hydrolysis	687:700	the hydrolysis of waste coffee polysaccharides from soluble coffee processing	687:763	This work describes the production of hydrolytic enzymes by solid-state fermentation (SSF), cultivating filamentous fungi on waste from the coffee industry, followed by their application in the hydrolysis of waste coffee polysaccharides from soluble coffee processing.
32033042	3	25	from	processing	754:763	arg1	polysaccharides					718:732	waste coffee polysaccharides	705:732	waste coffee polysaccharides from soluble coffee processing	705:763	This work describes the production of hydrolytic enzymes by solid-state fermentation (SSF), cultivating filamentous fungi on waste from the coffee industry, followed by their application in the hydrolysis of waste coffee polysaccharides from soluble coffee processing.
32033042	7	26	theme	coffee	1178:1183	arg1	waste					1185:1189	coffee waste	1178:1189	coffee waste	1178:1189	The best β-mannanase production results were found for a substrate composed of a mixture of coffee waste and wheat bran (1:1 w/w), using Aspergillusniger F12.
32033042	6	27	theme	substrate	1075:1083	arg1	aeration					1059:1066	better aeration	1052:1066	better aeration of the substrate	1052:1083	Higher enzyme yield was achieved in the bioreactor, due to better aeration of the substrate.
32033042	0	28	theme	β-Mannanase	0:10	arg1	Production					12:21	β-Mannanase Production	0:21	β-Mannanase Production	0:21	β-Mannanase Production Using Coffee Industry Waste for Application in Soluble Coffee Processing.
32033042	6	29	theme	enzyme	1000:1005	arg1	yield					1007:1011	Higher enzyme yield	993:1011	Higher enzyme yield	993:1011	Higher enzyme yield was achieved in the bioreactor, due to better aeration of the substrate.
32033042	4	30	theme	fermentation	858:869	arg1	conditions					871:880	the fermentation conditions	854:880	the fermentation conditions	854:880	Different substrate compositions were studied, an ideal microorganism was selected, and the fermentation conditions were optimized.
32033042	2	31	theme	sustainable	302:312	arg1	hydrolysis					201:210	The hydrolysis	197:210	The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes,	197:277	The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes, is an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts.
32033042	2	31	theme	sustainable	302:312	arg1	way					314:316	an eco-friendly and sustainable way	282:316	an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts	282:494	The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes, is an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts.
32033042	10	32	theme	coffee	1726:1731	arg1	industry					1733:1740	the coffee industry	1722:1740	the coffee industry	1722:1740	The production of β-mannanase by SSF and its application in the hydrolysis of coffee polysaccharides showed promise for improving soluble coffee processing, offering an attractive way to assist in closing the loops in the coffee industry and creating a circular economy.
32033042	0	33	theme	Coffee	29:34	arg1	Waste					45:49	Coffee Industry Waste	29:49	Coffee Industry Waste for Application in Soluble Coffee Processing	29:94	β-Mannanase Production Using Coffee Industry Waste for Application in Soluble Coffee Processing.
32033042	6	34	theme	Higher	993:998	arg1	yield					1007:1011	Higher enzyme yield	993:1011	Higher enzyme yield	993:1011	Higher enzyme yield was achieved in the bioreactor, due to better aeration of the substrate.
32033042	8	35	theme	considerable	1340:1351	arg1	amount					1353:1358	a considerable amount	1338:1358	a considerable amount of the carbohydrates in the coffee	1338:1393	The enzymatic extract proved to be very stable for 24 h, at 50 °C, and was able to hydrolyze a considerable amount of the carbohydrates in the coffee.
32033042	8	35	theme	considerable	1340:1351	arg1	carbohydrates					1367:1379	the carbohydrates	1363:1379	the carbohydrates in the coffee	1363:1393	The enzymatic extract proved to be very stable for 24 h, at 50 °C, and was able to hydrolyze a considerable amount of the carbohydrates in the coffee.
32033042	3	36	theme	solid-state	557:567	arg1	fermentation					569:580	solid-state fermentation	557:580	solid-state fermentation (SSF)	557:586	This work describes the production of hydrolytic enzymes by solid-state fermentation (SSF), cultivating filamentous fungi on waste from the coffee industry, followed by their application in the hydrolysis of waste coffee polysaccharides from soluble coffee processing.
32033042	3	36	theme	solid-state	557:567	arg1	SSF					583:585	SSF	583:585	SSF	583:585	This work describes the production of hydrolytic enzymes by solid-state fermentation (SSF), cultivating filamentous fungi on waste from the coffee industry, followed by their application in the hydrolysis of waste coffee polysaccharides from soluble coffee processing.
32033042	8	37	from	carbohydrates	1367:1379	arg1	coffee					1388:1393	the coffee	1384:1393	the coffee	1384:1393	The enzymatic extract proved to be very stable for 24 h, at 50 °C, and was able to hydrolyze a considerable amount of the carbohydrates in the coffee.
32033042	2	38	theme	processes	413:421	arg1	implementation					384:397	the implementation	380:397	the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts	380:494	The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes, is an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts.
32033042	1	39	theme	Soluble	97:103	arg1	coffee					105:110	Soluble coffee	97:110	Soluble coffee	97:110	Soluble coffee offers the combined benefits of high added value and practicality for its consumers.
32033042	3	40	theme	soluble	739:745	arg1	processing					754:763	soluble coffee processing	739:763	soluble coffee processing	739:763	This work describes the production of hydrolytic enzymes by solid-state fermentation (SSF), cultivating filamentous fungi on waste from the coffee industry, followed by their application in the hydrolysis of waste coffee polysaccharides from soluble coffee processing.
32033042	10	41	theme	attractive	1673:1682	arg1	way					1684:1686	an attractive way	1670:1686	an attractive way to assist in closing the loops in the coffee industry and creating a circular economy	1670:1772	The production of β-mannanase by SSF and its application in the hydrolysis of coffee polysaccharides showed promise for improving soluble coffee processing, offering an attractive way to assist in closing the loops in the coffee industry and creating a circular economy.
32033042	2	42	theme	eco-friendly	285:296	arg1	hydrolysis					201:210	The hydrolysis	197:210	The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes,	197:277	The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes, is an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts.
32033042	2	42	theme	eco-friendly	285:296	arg1	way					314:316	an eco-friendly and sustainable way	282:316	an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts	282:494	The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes, is an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts.
32033042	2	43	theme	industrial	402:411	arg1	processes					413:421	industrial processes	402:421	industrial processes that have lower energy requirements and can reduce environmental impacts	402:494	The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes, is an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts.
32033042	3	44	theme	coffee	747:752	arg1	processing					754:763	soluble coffee processing	739:763	soluble coffee processing	739:763	This work describes the production of hydrolytic enzymes by solid-state fermentation (SSF), cultivating filamentous fungi on waste from the coffee industry, followed by their application in the hydrolysis of waste coffee polysaccharides from soluble coffee processing.
32033042	3	45	from	application	672:682	arg1	hydrolysis					691:700	the hydrolysis	687:700	the hydrolysis of waste coffee polysaccharides from soluble coffee processing	687:763	This work describes the production of hydrolytic enzymes by solid-state fermentation (SSF), cultivating filamentous fungi on waste from the coffee industry, followed by their application in the hydrolysis of waste coffee polysaccharides from soluble coffee processing.
32033042	7	46	theme	waste	1185:1189	arg1	mixture					1167:1173	a mixture	1165:1173	a mixture of coffee waste and wheat bran (1:1 w/w)	1165:1214	The best β-mannanase production results were found for a substrate composed of a mixture of coffee waste and wheat bran (1:1 w/w), using Aspergillusniger F12.
32033042	10	47	theme	β-mannanase	1522:1532	arg1	application					1549:1559	its application	1545:1559	its application in the hydrolysis of coffee polysaccharides	1545:1603	The production of β-mannanase by SSF and its application in the hydrolysis of coffee polysaccharides showed promise for improving soluble coffee processing, offering an attractive way to assist in closing the loops in the coffee industry and creating a circular economy.
32033042	10	47	theme	β-mannanase	1522:1532	arg1	production					1508:1517	The production	1504:1517	The production of β-mannanase by SSF	1504:1539	The production of β-mannanase by SSF and its application in the hydrolysis of coffee polysaccharides showed promise for improving soluble coffee processing, offering an attractive way to assist in closing the loops in the coffee industry and creating a circular economy.
32033042	6	48	theme	better	1052:1057	arg1	aeration					1059:1066	better aeration	1052:1066	better aeration of the substrate	1052:1083	Higher enzyme yield was achieved in the bioreactor, due to better aeration of the substrate.
32033042	0	49	theme	Industry	36:43	arg1	Waste					45:49	Coffee Industry Waste	29:49	Coffee Industry Waste for Application in Soluble Coffee Processing	29:94	β-Mannanase Production Using Coffee Industry Waste for Application in Soluble Coffee Processing.
32033042	9	50	theme	cocktail	1435:1442	arg1	addition					1400:1407	The addition	1396:1407	The addition of a commercial cellulase cocktail to the crude extract	1396:1463	The addition of a commercial cellulase cocktail to the crude extract increased the hydrolysis yield by 56%.
32033042	3	51	theme	filamentous	601:611	arg1	fungi					613:617	filamentous fungi	601:617	filamentous fungi	601:617	This work describes the production of hydrolytic enzymes by solid-state fermentation (SSF), cultivating filamentous fungi on waste from the coffee industry, followed by their application in the hydrolysis of waste coffee polysaccharides from soluble coffee processing.
32033042	9	52	theme	commercial	1414:1423	arg1	cocktail					1435:1442	a commercial cellulase cocktail	1412:1442	a commercial cellulase cocktail	1412:1442	The addition of a commercial cellulase cocktail to the crude extract increased the hydrolysis yield by 56%.
32033042	3	53	from	hydrolysis	691:700	arg1	processing					754:763	soluble coffee processing	739:763	soluble coffee processing	739:763	This work describes the production of hydrolytic enzymes by solid-state fermentation (SSF), cultivating filamentous fungi on waste from the coffee industry, followed by their application in the hydrolysis of waste coffee polysaccharides from soluble coffee processing.
32033042	3	54	theme	coffee	637:642	arg1	industry					644:651	the coffee industry	633:651	the coffee industry	633:651	This work describes the production of hydrolytic enzymes by solid-state fermentation (SSF), cultivating filamentous fungi on waste from the coffee industry, followed by their application in the hydrolysis of waste coffee polysaccharides from soluble coffee processing.
32033042	2	55	theme	polysaccharides	222:236	arg1	hydrolysis					201:210	The hydrolysis	197:210	The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes,	197:277	The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes, is an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts.
32033042	2	55	theme	polysaccharides	222:236	arg1	way					314:316	an eco-friendly and sustainable way	282:316	an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts	282:494	The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes, is an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts.
32033042	3	56	theme	waste	705:709	arg1	polysaccharides					718:732	waste coffee polysaccharides	705:732	waste coffee polysaccharides from soluble coffee processing	705:763	This work describes the production of hydrolytic enzymes by solid-state fermentation (SSF), cultivating filamentous fungi on waste from the coffee industry, followed by their application in the hydrolysis of waste coffee polysaccharides from soluble coffee processing.
32033042	9	57	theme	cellulase	1425:1433	arg1	cocktail					1435:1442	a commercial cellulase cocktail	1412:1442	a commercial cellulase cocktail	1412:1442	The addition of a commercial cellulase cocktail to the crude extract increased the hydrolysis yield by 56%.
32033042	9	58	theme	hydrolysis	1479:1488	arg1	yield					1490:1494	the hydrolysis yield	1475:1494	the hydrolysis yield	1475:1494	The addition of a commercial cellulase cocktail to the crude extract increased the hydrolysis yield by 56%.
32033042	2	59	theme	coffee	215:220	arg1	polysaccharides					222:236	coffee polysaccharides	215:236	coffee polysaccharides	215:236	The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes, is an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts.
32033042	1	60	theme	combined	123:130	arg1	benefits					132:139	the combined benefits	119:139	the combined benefits of high added value and practicality for its consumers	119:194	Soluble coffee offers the combined benefits of high added value and practicality for its consumers.
32033042	8	61	theme	carbohydrates	1367:1379	arg1	amount					1353:1358	a considerable amount	1338:1358	a considerable amount of the carbohydrates in the coffee	1338:1393	The enzymatic extract proved to be very stable for 24 h, at 50 °C, and was able to hydrolyze a considerable amount of the carbohydrates in the coffee.
32033042	8	61	theme	carbohydrates	1367:1379	arg1	carbohydrates					1367:1379	the carbohydrates	1363:1379	the carbohydrates in the coffee	1363:1393	The enzymatic extract proved to be very stable for 24 h, at 50 °C, and was able to hydrolyze a considerable amount of the carbohydrates in the coffee.
32033042	10	62	from	loops	1713:1717	arg1	industry					1733:1740	the coffee industry	1722:1740	the coffee industry	1722:1740	The production of β-mannanase by SSF and its application in the hydrolysis of coffee polysaccharides showed promise for improving soluble coffee processing, offering an attractive way to assist in closing the loops in the coffee industry and creating a circular economy.
32033042	10	63	theme	soluble	1634:1640	arg1	processing					1649:1658	soluble coffee processing	1634:1658	soluble coffee processing	1634:1658	The production of β-mannanase by SSF and its application in the hydrolysis of coffee polysaccharides showed promise for improving soluble coffee processing, offering an attractive way to assist in closing the loops in the coffee industry and creating a circular economy.
32033042	8	64	from	°C	1308:1309	arg1	able					1320:1323	able	1320:1323	able	1320:1323	The enzymatic extract proved to be very stable for 24 h, at 50 °C, and was able to hydrolyze a considerable amount of the carbohydrates in the coffee.
32033042	8	64	from	°C	1308:1309	arg1	stable					1285:1290	stable	1285:1290	stable	1285:1290	The enzymatic extract proved to be very stable for 24 h, at 50 °C, and was able to hydrolyze a considerable amount of the carbohydrates in the coffee.
32033042	8	64	from	°C	1308:1309	arg1	extract					1259:1265	The enzymatic extract	1245:1265	The enzymatic extract	1245:1265	The enzymatic extract proved to be very stable for 24 h, at 50 °C, and was able to hydrolyze a considerable amount of the carbohydrates in the coffee.
32033042	6	65	theme	due	1045:1047	arg1	bioreactor					1033:1042	the bioreactor	1029:1042	the bioreactor	1029:1042	Higher enzyme yield was achieved in the bioreactor, due to better aeration of the substrate.
32033042	3	66	theme	hydrolytic	535:544	arg1	enzymes					546:552	hydrolytic enzymes	535:552	hydrolytic enzymes	535:552	This work describes the production of hydrolytic enzymes by solid-state fermentation (SSF), cultivating filamentous fungi on waste from the coffee industry, followed by their application in the hydrolysis of waste coffee polysaccharides from soluble coffee processing.
32033042	10	67	from	production	1508:1517	arg1	hydrolysis					1568:1577	the hydrolysis	1564:1577	the hydrolysis of coffee polysaccharides	1564:1603	The production of β-mannanase by SSF and its application in the hydrolysis of coffee polysaccharides showed promise for improving soluble coffee processing, offering an attractive way to assist in closing the loops in the coffee industry and creating a circular economy.
32033042	4	68	theme	substrate	776:784	arg1	compositions					786:797	Different substrate compositions	766:797	Different substrate compositions	766:797	Different substrate compositions were studied, an ideal microorganism was selected, and the fermentation conditions were optimized.
32033042	10	69	theme	polysaccharides	1589:1603	arg1	hydrolysis					1568:1577	the hydrolysis	1564:1577	the hydrolysis of coffee polysaccharides	1564:1603	The production of β-mannanase by SSF and its application in the hydrolysis of coffee polysaccharides showed promise for improving soluble coffee processing, offering an attractive way to assist in closing the loops in the coffee industry and creating a circular economy.
32033042	1	70	theme	added	149:153	arg1	value					155:159	high added value	144:159	high added value	144:159	Soluble coffee offers the combined benefits of high added value and practicality for its consumers.
32033042	7	71	theme	β-mannanase	1095:1105	arg1	results					1118:1124	The best β-mannanase production results	1086:1124	The best β-mannanase production results	1086:1124	The best β-mannanase production results were found for a substrate composed of a mixture of coffee waste and wheat bran (1:1 w/w), using Aspergillusniger F12.
32033042	0	72	theme	Soluble	70:76	arg1	Processing					85:94	Soluble Coffee Processing	70:94	Soluble Coffee Processing	70:94	β-Mannanase Production Using Coffee Industry Waste for Application in Soluble Coffee Processing.
32033042	5	73	theme	enzymes	915:921	arg1	production					923:932	enzymes production	915:932	enzymes production	915:932	Cultivations for enzymes production were carried out in flasks and in a packed-bed bioreactor.
32033042	4	74	theme	ideal	816:820	arg1	microorganism					822:834	an ideal microorganism	813:834	an ideal microorganism	813:834	Different substrate compositions were studied, an ideal microorganism was selected, and the fermentation conditions were optimized.
32033042	8	75	theme	enzymatic	1249:1257	arg1	able					1320:1323	able	1320:1323	able	1320:1323	The enzymatic extract proved to be very stable for 24 h, at 50 °C, and was able to hydrolyze a considerable amount of the carbohydrates in the coffee.
32033042	8	75	theme	enzymatic	1249:1257	arg1	stable					1285:1290	stable	1285:1290	stable	1285:1290	The enzymatic extract proved to be very stable for 24 h, at 50 °C, and was able to hydrolyze a considerable amount of the carbohydrates in the coffee.
32033042	8	75	theme	enzymatic	1249:1257	arg1	extract					1259:1265	The enzymatic extract	1245:1265	The enzymatic extract	1245:1265	The enzymatic extract proved to be very stable for 24 h, at 50 °C, and was able to hydrolyze a considerable amount of the carbohydrates in the coffee.
32033042	1	76	theme	high	144:147	arg1	value					155:159	high added value	144:159	high added value	144:159	Soluble coffee offers the combined benefits of high added value and practicality for its consumers.
32033042	10	77	theme	coffee	1582:1587	arg1	polysaccharides					1589:1603	coffee polysaccharides	1582:1603	coffee polysaccharides	1582:1603	The production of β-mannanase by SSF and its application in the hydrolysis of coffee polysaccharides showed promise for improving soluble coffee processing, offering an attractive way to assist in closing the loops in the coffee industry and creating a circular economy.
32033042	2	78	theme	biochemical	245:255	arg1	route					257:261	the biochemical route	241:261	the biochemical route	241:261	The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes, is an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts.
32033042	2	79	theme	product	349:355	arg1	quality					333:339	the quality	329:339	the quality of this product	329:355	The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes, is an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts.
32172860	7	0	dep	released	1061:1068	arg1	higher					1054:1059	higher	1054:1059	higher	1054:1059	Similarly, the kinetics of insuline release, through the FRP/Cs composed hydrogels exhibited higher released amounts in acidic systems.
32172860	1	1	theme	medical	197:203	arg1	applications					205:216	medical applications	197:216	medical applications	197:216	Hydrogels properties open up many possibilities for medical applications.
32172860	5	2	theme	ionic	773:777	arg1	strength					779:786	ionic strength	773:786	ionic strength	773:786	Moreover, the prepared hydrogels are simultaneous sensitive to pH, ionic strength and temperature as demonstrated in the swelling ratio test.
32172860	2	3	dep	in	303:304	arg1	vitro					306:310	vitro	306:310	vitro	306:310	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	4	4	theme	FRP	589:591	arg1	addition					593:600	FRP addition	589:600	FRP addition	589:600	Data revealed that FRP addition enhanced the water holding capacity and the water uptake percentages, as well as the textural behavior.
32172860	0	5	theme	controlled-release	125:142	arg1	insulin					117:123	insulin controlled-release	117:142	insulin controlled-release	117:142	Conception and characterization of a multi-sensitive composite chitosan-red marine alga-polysaccharide hydrogels for insulin controlled-release.
32172860	3	6	theme	antioxidant	546:556	arg1	properties					558:567	structural, morphological, thermal and antioxidant properties	507:567	structural, morphological, thermal and antioxidant properties	507:567	The FRP/Cs composed hydrogels were characterized in terms of structural, morphological, thermal and antioxidant properties.
32172860	2	7	theme	release	312:318	arg1	behavior					320:327	the in vitro release behavior	299:327	the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP)	299:443	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	8	8	theme	suitable	1149:1156	arg1	microenvironment					1172:1187	suitable and promising microenvironment	1149:1187	suitable and promising microenvironment	1149:1187	Through this study, the prepared hydrogels provided suitable and promising microenvironment in drugs delivery.
32172860	6	9	theme	greater	896:902	arg1	degradation					904:914	greater degradation	896:914	greater degradation	896:914	Additionally, hydrogels at pH 1.2 PBS underwent greater degradation, compared to samples immersed in pH 7.4 PBS.
32172860	3	10	theme	properties	558:567	arg1	terms					498:502	terms	498:502	terms of structural, morphological, thermal and antioxidant properties	498:567	The FRP/Cs composed hydrogels were characterized in terms of structural, morphological, thermal and antioxidant properties.
32172860	2	11	theme	in	303:304	arg1	behavior					320:327	the in vitro release behavior	299:327	the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP)	299:443	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	7	12	from	amounts	1070:1076	arg1	systems					1088:1094	acidic systems	1081:1094	acidic systems	1081:1094	Similarly, the kinetics of insuline release, through the FRP/Cs composed hydrogels exhibited higher released amounts in acidic systems.
32172860	3	13	theme	thermal	534:540	arg1	properties					558:567	structural, morphological, thermal and antioxidant properties	507:567	structural, morphological, thermal and antioxidant properties	507:567	The FRP/Cs composed hydrogels were characterized in terms of structural, morphological, thermal and antioxidant properties.
32172860	2	14	theme	macroalga	390:398	arg1	FRP					440:442	FRP	440:442	FRP	440:442	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	2	14	theme	macroalga	390:398	arg1	polysaccharide					424:437	red marine macroalga Falkenbergia rufolanosa polysaccharide	379:437	red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP)	379:443	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	8	15	theme	promising	1162:1170	arg1	microenvironment					1172:1187	suitable and promising microenvironment	1149:1187	suitable and promising microenvironment	1149:1187	Through this study, the prepared hydrogels provided suitable and promising microenvironment in drugs delivery.
32172860	2	16	theme	marine	383:388	arg1	FRP					440:442	FRP	440:442	FRP	440:442	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	2	16	theme	marine	383:388	arg1	polysaccharide					424:437	red marine macroalga Falkenbergia rufolanosa polysaccharide	379:437	red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP)	379:443	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	8	17	theme	drugs	1192:1196	arg1	delivery					1198:1205	drugs delivery	1192:1205	drugs delivery	1192:1205	Through this study, the prepared hydrogels provided suitable and promising microenvironment in drugs delivery.
32172860	8	18	theme	prepared	1121:1128	arg1	hydrogels					1130:1138	the prepared hydrogels	1117:1138	the prepared hydrogels	1117:1138	Through this study, the prepared hydrogels provided suitable and promising microenvironment in drugs delivery.
32172860	2	19	theme	hydrogels	332:340	arg1	behavior					320:327	the in vitro release behavior	299:327	the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP)	299:443	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	2	20	theme	red	379:381	arg1	FRP					440:442	FRP	440:442	FRP	440:442	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	2	20	theme	red	379:381	arg1	polysaccharide					424:437	red marine macroalga Falkenbergia rufolanosa polysaccharide	379:437	red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP)	379:443	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	6	21	from	PBS	882:884	arg1	hydrogels					862:870	hydrogels	862:870	hydrogels at pH 1.2 PBS	862:884	Additionally, hydrogels at pH 1.2 PBS underwent greater degradation, compared to samples immersed in pH 7.4 PBS.
32172860	7	22	theme	acidic	1081:1086	arg1	systems					1088:1094	acidic systems	1081:1094	acidic systems	1081:1094	Similarly, the kinetics of insuline release, through the FRP/Cs composed hydrogels exhibited higher released amounts in acidic systems.
32172860	7	23	theme	released	1061:1068	arg1	amounts					1070:1076	higher released amounts	1054:1076	higher released amounts in acidic systems	1054:1094	Similarly, the kinetics of insuline release, through the FRP/Cs composed hydrogels exhibited higher released amounts in acidic systems.
32172860	4	24	theme	water	646:650	arg1	percentages					659:669	the water uptake percentages	642:669	the water uptake percentages	642:669	Data revealed that FRP addition enhanced the water holding capacity and the water uptake percentages, as well as the textural behavior.
32172860	5	25	theme	swelling	827:834	arg1	test					842:845	the swelling ratio test	823:845	the swelling ratio test	823:845	Moreover, the prepared hydrogels are simultaneous sensitive to pH, ionic strength and temperature as demonstrated in the swelling ratio test.
32172860	2	26	theme	present	226:232	arg1	study					234:238	the present study	222:238	the present study	222:238	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	1	27	theme	Hydrogels	145:153	arg1	properties					155:164	Hydrogels properties	145:164	Hydrogels properties	145:164	Hydrogels properties open up many possibilities for medical applications.
32172860	4	28	theme	textural	687:694	arg1	behavior					696:703	the textural behavior	683:703	the textural behavior	683:703	Data revealed that FRP addition enhanced the water holding capacity and the water uptake percentages, as well as the textural behavior.
32172860	0	29	theme	multi-sensitive	37:51	arg1	alga-polysaccharide					83:101	a multi-sensitive composite chitosan-red marine alga-polysaccharide	35:101	a multi-sensitive composite chitosan-red marine alga-polysaccharide	35:101	Conception and characterization of a multi-sensitive composite chitosan-red marine alga-polysaccharide hydrogels for insulin controlled-release.
32172860	2	30	theme	rufolanosa	413:422	arg1	FRP					440:442	FRP	440:442	FRP	440:442	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	2	30	theme	rufolanosa	413:422	arg1	polysaccharide					424:437	red marine macroalga Falkenbergia rufolanosa polysaccharide	379:437	red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP)	379:443	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	6	31	theme	pH	949:950	arg1	PBS					956:958	pH 7.4 PBS	949:958	pH 7.4 PBS	949:958	Additionally, hydrogels at pH 1.2 PBS underwent greater degradation, compared to samples immersed in pH 7.4 PBS.
32172860	5	32	theme	prepared	720:727	arg1	hydrogels					729:737	the prepared hydrogels	716:737	the prepared hydrogels	716:737	Moreover, the prepared hydrogels are simultaneous sensitive to pH, ionic strength and temperature as demonstrated in the swelling ratio test.
32172860	5	32	theme	prepared	720:727	arg1	sensitive					756:764	sensitive	756:764	sensitive	756:764	Moreover, the prepared hydrogels are simultaneous sensitive to pH, ionic strength and temperature as demonstrated in the swelling ratio test.
32172860	2	33	theme	Falkenbergia	400:411	arg1	FRP					440:442	FRP	440:442	FRP	440:442	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	2	33	theme	Falkenbergia	400:411	arg1	polysaccharide					424:437	red marine macroalga Falkenbergia rufolanosa polysaccharide	379:437	red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP)	379:443	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	0	34	theme	chitosan-red	63:74	arg1	alga-polysaccharide					83:101	a multi-sensitive composite chitosan-red marine alga-polysaccharide	35:101	a multi-sensitive composite chitosan-red marine alga-polysaccharide	35:101	Conception and characterization of a multi-sensitive composite chitosan-red marine alga-polysaccharide hydrogels for insulin controlled-release.
32172860	3	35	theme	structural	507:516	arg1	properties					558:567	structural, morphological, thermal and antioxidant properties	507:567	structural, morphological, thermal and antioxidant properties	507:567	The FRP/Cs composed hydrogels were characterized in terms of structural, morphological, thermal and antioxidant properties.
32172860	6	36	theme	pH	875:876	arg1	PBS					882:884	pH 1.2 PBS	875:884	pH 1.2 PBS	875:884	Additionally, hydrogels at pH 1.2 PBS underwent greater degradation, compared to samples immersed in pH 7.4 PBS.
32172860	2	37	theme	crab	356:359	arg1	Cs					371:372	Cs	371:372	Cs	371:372	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	2	37	theme	crab	356:359	arg1	chitosan					361:368	blue crab chitosan	351:368	blue crab chitosan (Cs)	351:373	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	0	38	theme	composite	53:61	arg1	alga-polysaccharide					83:101	a multi-sensitive composite chitosan-red marine alga-polysaccharide	35:101	a multi-sensitive composite chitosan-red marine alga-polysaccharide	35:101	Conception and characterization of a multi-sensitive composite chitosan-red marine alga-polysaccharide hydrogels for insulin controlled-release.
32172860	2	39	theme	drug	249:252	arg1	insulin					254:260	protein drug insulin	241:260	protein drug insulin	241:260	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	2	39	theme	drug	249:252	arg1	drug					286:289	a model drug	278:289	a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP)	278:443	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	7	40	theme	insuline	988:995	arg1	release					997:1003	insuline release	988:1003	insuline release	988:1003	Similarly, the kinetics of insuline release, through the FRP/Cs composed hydrogels exhibited higher released amounts in acidic systems.
32172860	2	41	theme	blue	351:354	arg1	crab					356:359	blue crab	351:359	blue crab chitosan (Cs)	351:373	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	2	42	theme	protein	241:247	arg1	insulin					254:260	protein drug insulin	241:260	protein drug insulin	241:260	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	2	42	theme	protein	241:247	arg1	drug					286:289	a model drug	278:289	a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP)	278:443	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	7	43	theme	release	997:1003	arg1	kinetics					976:983	the kinetics	972:983	the kinetics of insuline release, through the FRP/Cs composed hydrogels	972:1042	Similarly, the kinetics of insuline release, through the FRP/Cs composed hydrogels exhibited higher released amounts in acidic systems.
32172860	4	44	theme	holding	621:627	arg1	capacity					629:636	the water holding capacity	611:636	the water holding capacity	611:636	Data revealed that FRP addition enhanced the water holding capacity and the water uptake percentages, as well as the textural behavior.
32172860	1	45	theme	many	174:177	arg1	possibilities					179:191	many possibilities	174:191	many possibilities for medical applications	174:216	Hydrogels properties open up many possibilities for medical applications.
32172860	4	46	theme	water	615:619	arg1	capacity					629:636	the water holding capacity	611:636	the water holding capacity	611:636	Data revealed that FRP addition enhanced the water holding capacity and the water uptake percentages, as well as the textural behavior.
32172860	0	47	theme	alga-polysaccharide	83:101	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Conception and characterization of a multi-sensitive composite chitosan-red marine alga-polysaccharide hydrogels for insulin controlled-release.
32172860	0	47	theme	alga-polysaccharide	83:101	arg1	Conception					0:9	Conception	0:9	Conception	0:9	Conception and characterization of a multi-sensitive composite chitosan-red marine alga-polysaccharide hydrogels for insulin controlled-release.
32172860	4	48	theme	uptake	652:657	arg1	percentages					659:669	the water uptake percentages	642:669	the water uptake percentages	642:669	Data revealed that FRP addition enhanced the water holding capacity and the water uptake percentages, as well as the textural behavior.
32172860	2	49	theme	model	280:284	arg1	insulin					254:260	protein drug insulin	241:260	protein drug insulin	241:260	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	2	49	theme	model	280:284	arg1	drug					286:289	a model drug	278:289	a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP)	278:443	In the present study, protein drug insulin was selected as a model drug to test the in vitro release behavior of hydrogels based on blue crab chitosan (Cs) and red marine macroalga Falkenbergia rufolanosa polysaccharide (FRP).
32172860	5	50	theme	ratio	836:840	arg1	test					842:845	the swelling ratio test	823:845	the swelling ratio test	823:845	Moreover, the prepared hydrogels are simultaneous sensitive to pH, ionic strength and temperature as demonstrated in the swelling ratio test.
32172860	3	51	theme	morphological	519:531	arg1	properties					558:567	structural, morphological, thermal and antioxidant properties	507:567	structural, morphological, thermal and antioxidant properties	507:567	The FRP/Cs composed hydrogels were characterized in terms of structural, morphological, thermal and antioxidant properties.
32172860	0	52	theme	marine	76:81	arg1	alga-polysaccharide					83:101	a multi-sensitive composite chitosan-red marine alga-polysaccharide	35:101	a multi-sensitive composite chitosan-red marine alga-polysaccharide	35:101	Conception and characterization of a multi-sensitive composite chitosan-red marine alga-polysaccharide hydrogels for insulin controlled-release.
34213249	6	0	theme	sugar	942:946	arg1	compounds					948:956	four polar sugar compounds	931:956	four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid)	931:1119	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	6	0	theme	sugar	942:946	arg1	dihydrat					1017:1024	D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat	959:1024	D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat	959:1024	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	6	0	theme	sugar	942:946	arg1	raffinose					1030:1038	raffinose	1030:1038	raffinose	1030:1038	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	3	1	theme	structure	586:594	arg1	analysis					596:603	pore structure analysis	581:603	pore structure analysis	581:603	Surface area (302 m2/g), mean pore diameter (9.5 nm), and pore volume (0.63 cm3/g) of Sil@MC materials were also measured by pore structure analysis.
34213249	6	2	theme	0.1	1097:1099	arg1	%					1100:1100	%	1100:1100	%	1100:1100	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	1	3	theme	formaldehyde	255:266	arg1	PF					277:278	PF	277:278	PF	277:278	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	1	3	theme	formaldehyde	255:266	arg1	polymer					268:274	the phenol formaldehyde polymer	244:274	the phenol formaldehyde polymer (PF) on SiO2 surface	244:295	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	8	4	theme	Sil	1523:1525	arg1	column					1530:1535	the Sil@MC column	1519:1535	the Sil@MC column with good peak shapes	1519:1557	Finally, the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose, were successfully separated by the Sil@MC column with good peak shapes.
34213249	6	5	dep	%	1100:1100	arg1	v/v					1103:1105	v/v	1103:1105	v/v	1103:1105	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	2	6	contain	had	431:433	arg1	it					428:429	it	428:429	it	428:429	The morphology observation of the Sil@MC stationary phase showed that it had good monodispersity.
34213249	2	6	contain	had	431:433	arg2	monodispersity					440:453	good monodispersity	435:453	good monodispersity	435:453	The morphology observation of the Sil@MC stationary phase showed that it had good monodispersity.
34213249	8	7	theme	MC	1527:1528	arg1	column					1530:1535	the Sil@MC column	1519:1535	the Sil@MC column with good peak shapes	1519:1557	Finally, the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose, were successfully separated by the Sil@MC column with good peak shapes.
34213249	1	8	theme	Sil	199:201	arg1	MC					203:204	Sil@MC	199:204	Sil@MC	199:204	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	1	8	theme	Sil	199:201	arg1	microspheres					185:196	core-shell mesoporous silica-carbon composite microspheres	139:196	core-shell mesoporous silica-carbon composite microspheres (Sil@MC)	139:205	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	6	9	with	compounds	948:956	arg1	phase					1057:1061	the mobile phase	1046:1061	the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid)	1046:1119	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	6	10	theme	mobile	1050:1055	arg1	phase					1057:1061	the mobile phase	1046:1061	the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid)	1046:1119	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	5	11	theme	Sil	769:771	arg1	column					776:781	the Sil@MC column	765:781	the Sil@MC column	765:781	As a stationary phase of HPLC, the Sil@MC column was filled by a slurry method.
34213249	5	11	theme	Sil	769:771	arg1	phase					750:754	a stationary phase	737:754	a stationary phase of HPLC	737:762	As a stationary phase of HPLC, the Sil@MC column was filled by a slurry method.
34213249	8	12	theme	peak	1547:1550	arg1	shapes					1552:1557	good peak shapes	1542:1557	good peak shapes	1542:1557	Finally, the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose, were successfully separated by the Sil@MC column with good peak shapes.
34213249	5	13	theme	stationary	739:748	arg1	phase					750:754	a stationary phase	737:754	a stationary phase of HPLC	737:762	As a stationary phase of HPLC, the Sil@MC column was filled by a slurry method.
34213249	5	13	theme	stationary	739:748	arg1	column					776:781	the Sil@MC column	765:781	the Sil@MC column	765:781	As a stationary phase of HPLC, the Sil@MC column was filled by a slurry method.
34213249	6	14	theme	@	821:821	arg1	materials					825:833	The Sil@MC materials	814:833	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin	814:893	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	9	15	attach	derived	1615:1621	arg2	composites					1604:1613	silica-carbon composites	1590:1613	silica-carbon composites derived from phenolic resin	1590:1641	The results demonstrates that silica-carbon composites derived from phenolic resin have potential application in polar compounds chromatographic separation.
34213249	9	15	attach	derived	1615:1621	arg1	resin					1637:1641	phenolic resin	1628:1641	phenolic resin	1628:1641	The results demonstrates that silica-carbon composites derived from phenolic resin have potential application in polar compounds chromatographic separation.
34213249	1	16	from	coating	233:239	arg1	surface					289:295	SiO2 surface	284:295	SiO2 surface	284:295	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	3	17	theme	Sil	542:544	arg1	materials					549:557	Sil@MC materials	542:557	Sil@MC materials	542:557	Surface area (302 m2/g), mean pore diameter (9.5 nm), and pore volume (0.63 cm3/g) of Sil@MC materials were also measured by pore structure analysis.
34213249	5	18	theme	slurry	799:804	arg1	method					806:811	a slurry method	797:811	a slurry method	797:811	As a stationary phase of HPLC, the Sil@MC column was filled by a slurry method.
34213249	6	19	theme	phenolic	880:887	arg1	resin					889:893	phenolic resin	880:893	phenolic resin	880:893	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	6	20	theme	-trehalose	1006:1015	arg1	dihydrat					1017:1024	D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat	959:1024	D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat	959:1024	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	6	20	theme	-trehalose	1006:1015	arg1	compounds					948:956	four polar sugar compounds	931:956	four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid)	931:1119	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	9	21	contain	have	1643:1646	arg2	application					1658:1668	potential application	1648:1668	potential application	1648:1668	The results demonstrates that silica-carbon composites derived from phenolic resin have potential application in polar compounds chromatographic separation.
34213249	9	21	contain	have	1643:1646	arg1	composites					1604:1613	silica-carbon composites	1590:1613	silica-carbon composites derived from phenolic resin	1590:1641	The results demonstrates that silica-carbon composites derived from phenolic resin have potential application in polar compounds chromatographic separation.
34213249	5	22	theme	MC	773:774	arg1	column					776:781	the Sil@MC column	765:781	the Sil@MC column	765:781	As a stationary phase of HPLC, the Sil@MC column was filled by a slurry method.
34213249	5	22	theme	MC	773:774	arg1	phase					750:754	a stationary phase	737:754	a stationary phase of HPLC	737:762	As a stationary phase of HPLC, the Sil@MC column was filled by a slurry method.
34213249	8	23	theme	representative	1274:1287	arg1	isomers					1305:1311	the representative oligosaccharide isomers	1270:1311	the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose,	1270:1486	Finally, the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose, were successfully separated by the Sil@MC column with good peak shapes.
34213249	0	24	from	[Preparation	0:11	arg1	separation					111:120	saccharide separation	100:120	saccharide separation	100:120	[Preparation of core-shell silica-carbon composite microspheres stationary phase and application in saccharide separation].
34213249	1	25	theme	nitrogen	337:344	arg1	atmosphere					346:355	nitrogen atmosphere	337:355	nitrogen atmosphere	337:355	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	6	26	used	used	904:907	arg2	materials					825:833	The Sil@MC materials	814:833	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin	814:893	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	1	27	theme	core-shell	139:148	arg1	MC					203:204	Sil@MC	199:204	Sil@MC	199:204	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	1	27	theme	core-shell	139:148	arg1	microspheres					185:196	core-shell mesoporous silica-carbon composite microspheres	139:196	core-shell mesoporous silica-carbon composite microspheres (Sil@MC)	139:205	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	9	28	theme	silica-carbon	1590:1602	arg1	composites					1604:1613	silica-carbon composites	1590:1613	silica-carbon composites derived from phenolic resin	1590:1641	The results demonstrates that silica-carbon composites derived from phenolic resin have potential application in polar compounds chromatographic separation.
34213249	8	29	theme	raffinose	1316:1324	arg1	isomers					1305:1311	the representative oligosaccharide isomers	1270:1311	the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose,	1270:1486	Finally, the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose, were successfully separated by the Sil@MC column with good peak shapes.
34213249	8	30	theme	melezitose	1327:1336	arg1	isomers					1305:1311	the representative oligosaccharide isomers	1270:1311	the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose,	1270:1486	Finally, the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose, were successfully separated by the Sil@MC column with good peak shapes.
34213249	4	31	theme	@	637:637	arg1	MC					638:639	the Sil@MC	630:639	the Sil@MC	630:639	The results showed that the Sil@MC was successfully immobilized on the silica particles via copolymerization and carbonization.
34213249	9	32	theme	phenolic	1628:1635	arg1	resin					1637:1641	phenolic resin	1628:1641	phenolic resin	1628:1641	The results demonstrates that silica-carbon composites derived from phenolic resin have potential application in polar compounds chromatographic separation.
34213249	2	33	theme	stationary	399:408	arg1	phase					410:414	the Sil@MC stationary phase	388:414	the Sil@MC stationary phase	388:414	The morphology observation of the Sil@MC stationary phase showed that it had good monodispersity.
34213249	0	34	theme	core-shell	16:25	arg1	microspheres					51:62	core-shell silica-carbon composite microspheres stationary phase and application	16:95	core-shell silica-carbon composite microspheres stationary phase and application	16:95	[Preparation of core-shell silica-carbon composite microspheres stationary phase and application in saccharide separation].
34213249	0	34	theme	core-shell	16:25	arg1	application					85:95	application	85:95	application	85:95	[Preparation of core-shell silica-carbon composite microspheres stationary phase and application in saccharide separation].
34213249	0	34	theme	core-shell	16:25	arg1	phase					75:79	stationary phase	64:79	stationary phase	64:79	[Preparation of core-shell silica-carbon composite microspheres stationary phase and application in saccharide separation].
34213249	1	35	theme	mesoporous	150:159	arg1	MC					203:204	Sil@MC	199:204	Sil@MC	199:204	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	1	35	theme	mesoporous	150:159	arg1	microspheres					185:196	core-shell mesoporous silica-carbon composite microspheres	139:196	core-shell mesoporous silica-carbon composite microspheres (Sil@MC)	139:205	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	8	36	theme	nystose	1353:1359	arg1	isomers					1305:1311	the representative oligosaccharide isomers	1270:1311	the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose,	1270:1486	Finally, the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose, were successfully separated by the Sil@MC column with good peak shapes.
34213249	2	37	theme	@	395:395	arg1	phase					410:414	the Sil@MC stationary phase	388:414	the Sil@MC stationary phase	388:414	The morphology observation of the Sil@MC stationary phase showed that it had good monodispersity.
34213249	0	38	theme	composite	41:49	arg1	microspheres					51:62	core-shell silica-carbon composite microspheres stationary phase and application	16:95	core-shell silica-carbon composite microspheres stationary phase and application	16:95	[Preparation of core-shell silica-carbon composite microspheres stationary phase and application in saccharide separation].
34213249	0	38	theme	composite	41:49	arg1	application					85:95	application	85:95	application	85:95	[Preparation of core-shell silica-carbon composite microspheres stationary phase and application in saccharide separation].
34213249	0	38	theme	composite	41:49	arg1	phase					75:79	stationary phase	64:79	stationary phase	64:79	[Preparation of core-shell silica-carbon composite microspheres stationary phase and application in saccharide separation].
34213249	2	39	theme	good	435:438	arg1	monodispersity					440:453	good monodispersity	435:453	good monodispersity	435:453	The morphology observation of the Sil@MC stationary phase showed that it had good monodispersity.
34213249	3	40	theme	MC	546:547	arg1	materials					549:557	Sil@MC materials	542:557	Sil@MC materials	542:557	Surface area (302 m2/g), mean pore diameter (9.5 nm), and pore volume (0.63 cm3/g) of Sil@MC materials were also measured by pore structure analysis.
34213249	0	41	theme	stationary	64:73	arg1	microspheres					51:62	core-shell silica-carbon composite microspheres stationary phase and application	16:95	core-shell silica-carbon composite microspheres stationary phase and application	16:95	[Preparation of core-shell silica-carbon composite microspheres stationary phase and application in saccharide separation].
34213249	0	41	theme	stationary	64:73	arg1	phase					75:79	stationary phase	64:79	stationary phase	64:79	[Preparation of core-shell silica-carbon composite microspheres stationary phase and application in saccharide separation].
34213249	9	42	theme	polar	1673:1677	arg1	separation					1705:1714	polar compounds chromatographic separation	1673:1714	polar compounds chromatographic separation	1673:1714	The results demonstrates that silica-carbon composites derived from phenolic resin have potential application in polar compounds chromatographic separation.
34213249	8	43	theme	milk	1372:1375	arg1	isomers					1393:1399	human milk oligosaccharide isomers	1366:1399	human milk oligosaccharide isomers	1366:1399	Finally, the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose, were successfully separated by the Sil@MC column with good peak shapes.
34213249	3	44	theme	pore	581:584	arg1	analysis					596:603	pore structure analysis	581:603	pore structure analysis	581:603	Surface area (302 m2/g), mean pore diameter (9.5 nm), and pore volume (0.63 cm3/g) of Sil@MC materials were also measured by pore structure analysis.
34213249	3	45	theme	Surface	456:462	arg1	area					464:467	Surface area	456:467	Surface area (302 m2/g)	456:478	Surface area (302 m2/g), mean pore diameter (9.5 nm), and pore volume (0.63 cm3/g) of Sil@MC materials were also measured by pore structure analysis.
34213249	3	45	theme	Surface	456:462	arg1	m2/g					474:477	302 m2/g	470:477	302 m2/g	470:477	Surface area (302 m2/g), mean pore diameter (9.5 nm), and pore volume (0.63 cm3/g) of Sil@MC materials were also measured by pore structure analysis.
34213249	1	46	theme	one-step	224:231	arg1	coating					233:239	one-step coating	224:239	one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface	224:295	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	1	47	theme	silica-carbon	161:173	arg1	MC					203:204	Sil@MC	199:204	Sil@MC	199:204	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	1	47	theme	silica-carbon	161:173	arg1	microspheres					185:196	core-shell mesoporous silica-carbon composite microspheres	139:196	core-shell mesoporous silica-carbon composite microspheres (Sil@MC)	139:205	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	9	48	theme	chromatographic	1689:1703	arg1	separation					1705:1714	polar compounds chromatographic separation	1673:1714	polar compounds chromatographic separation	1673:1714	The results demonstrates that silica-carbon composites derived from phenolic resin have potential application in polar compounds chromatographic separation.
34213249	8	49	theme	isomers	1393:1399	arg1	isomers					1305:1311	the representative oligosaccharide isomers	1270:1311	the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose,	1270:1486	Finally, the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose, were successfully separated by the Sil@MC column with good peak shapes.
34213249	6	50	theme	compounds	948:956	arg1	separation					917:926	the separation	913:926	the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid)	913:1119	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	8	51	theme	such	1402:1405	arg1	isomers					1393:1399	human milk oligosaccharide isomers	1366:1399	human milk oligosaccharide isomers	1366:1399	Finally, the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose, were successfully separated by the Sil@MC column with good peak shapes.
34213249	6	52	theme	%	1100:1100	arg1	acid					1115:1118	0.1% (v/v) formic acid	1097:1118	0.1% (v/v) formic acid	1097:1118	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	1	53	theme	phenol	248:253	arg1	PF					277:278	PF	277:278	PF	277:278	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	1	53	theme	phenol	248:253	arg1	polymer					268:274	the phenol formaldehyde polymer	244:274	the phenol formaldehyde polymer (PF) on SiO2 surface	244:295	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	6	54	theme	polar	936:940	arg1	compounds					948:956	four polar sugar compounds	931:956	four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid)	931:1119	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	6	54	theme	polar	936:940	arg1	dihydrat					1017:1024	D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat	959:1024	D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat	959:1024	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	6	54	theme	polar	936:940	arg1	raffinose					1030:1038	raffinose	1030:1038	raffinose	1030:1038	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	1	55	from	surface	289:295	arg1	coating					233:239	one-step coating	224:239	one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface	224:295	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	1	56	theme	polymer	268:274	arg1	coating					233:239	one-step coating	224:239	one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface	224:295	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	7	57	theme	polar	1227:1231	arg1	compounds					1239:1247	these polar sugar compounds	1221:1247	these polar sugar compounds	1221:1247	However, the material formed by calcinating SiO2 without coating phenolic resin could not separate these polar sugar compounds by HPLC-MS.
34213249	6	58	theme	acetonitrile-water	1066:1083	arg1	phase					1057:1061	the mobile phase	1046:1061	the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid)	1046:1119	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	3	59	theme	pore	486:489	arg1	diameter					491:498	mean pore diameter	481:498	mean pore diameter (9.5 nm)	481:507	Surface area (302 m2/g), mean pore diameter (9.5 nm), and pore volume (0.63 cm3/g) of Sil@MC materials were also measured by pore structure analysis.
34213249	3	59	theme	pore	486:489	arg1	nm					505:506	9.5 nm	501:506	9.5 nm	501:506	Surface area (302 m2/g), mean pore diameter (9.5 nm), and pore volume (0.63 cm3/g) of Sil@MC materials were also measured by pore structure analysis.
34213249	5	60	theme	HPLC	759:762	arg1	phase					750:754	a stationary phase	737:754	a stationary phase of HPLC	737:762	As a stationary phase of HPLC, the Sil@MC column was filled by a slurry method.
34213249	5	60	theme	HPLC	759:762	arg1	column					776:781	the Sil@MC column	765:781	the Sil@MC column	765:781	As a stationary phase of HPLC, the Sil@MC column was filled by a slurry method.
34213249	7	61	theme	sugar	1233:1237	arg1	compounds					1239:1247	these polar sugar compounds	1221:1247	these polar sugar compounds	1221:1247	However, the material formed by calcinating SiO2 without coating phenolic resin could not separate these polar sugar compounds by HPLC-MS.
34213249	8	62	theme	@	1526:1526	arg1	column					1530:1535	the Sil@MC column	1519:1535	the Sil@MC column with good peak shapes	1519:1557	Finally, the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose, were successfully separated by the Sil@MC column with good peak shapes.
34213249	1	63	theme	@	202:202	arg1	MC					203:204	Sil@MC	199:204	Sil@MC	199:204	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	1	63	theme	@	202:202	arg1	microspheres					185:196	core-shell mesoporous silica-carbon composite microspheres	139:196	core-shell mesoporous silica-carbon composite microspheres (Sil@MC)	139:205	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	1	64	from	polymer	268:274	arg1	surface					289:295	SiO2 surface	284:295	SiO2 surface	284:295	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	6	65	theme	MC	822:823	arg1	materials					825:833	The Sil@MC materials	814:833	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin	814:893	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	6	66	theme	-glucosamine	964:975	arg1	hydrochloride					977:989	-glucosamine hydrochloride	964:989	-glucosamine hydrochloride	964:989	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	8	67	theme	good	1542:1545	arg1	shapes					1552:1557	good peak shapes	1542:1557	good peak shapes	1542:1557	Finally, the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose, were successfully separated by the Sil@MC column with good peak shapes.
34213249	6	68	theme	Sil	818:820	arg1	materials					825:833	The Sil@MC materials	814:833	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin	814:893	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	6	69	theme	formic	1108:1113	arg1	acid					1115:1118	0.1% (v/v) formic acid	1097:1118	0.1% (v/v) formic acid	1097:1118	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	3	70	theme	pore	514:517	arg1	volume					519:524	pore volume	514:524	pore volume (0.63 cm3/g)	514:537	Surface area (302 m2/g), mean pore diameter (9.5 nm), and pore volume (0.63 cm3/g) of Sil@MC materials were also measured by pore structure analysis.
34213249	3	70	theme	pore	514:517	arg1	cm3/g					532:536	0.63 cm3/g	527:536	0.63 cm3/g	527:536	Surface area (302 m2/g), mean pore diameter (9.5 nm), and pore volume (0.63 cm3/g) of Sil@MC materials were also measured by pore structure analysis.
34213249	7	71	theme	calcinating	1154:1164	arg1	SiO2					1166:1169	calcinating SiO2	1154:1169	calcinating SiO2	1154:1169	However, the material formed by calcinating SiO2 without coating phenolic resin could not separate these polar sugar compounds by HPLC-MS.
34213249	5	72	theme	@	772:772	arg1	column					776:781	the Sil@MC column	765:781	the Sil@MC column	765:781	As a stationary phase of HPLC, the Sil@MC column was filled by a slurry method.
34213249	5	72	theme	@	772:772	arg1	phase					750:754	a stationary phase	737:754	a stationary phase of HPLC	737:762	As a stationary phase of HPLC, the Sil@MC column was filled by a slurry method.
34213249	8	73	theme	oligosaccharide	1289:1303	arg1	isomers					1305:1311	the representative oligosaccharide isomers	1270:1311	the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose,	1270:1486	Finally, the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose, were successfully separated by the Sil@MC column with good peak shapes.
34213249	0	74	theme	saccharide	100:109	arg1	separation					111:120	saccharide separation	100:120	saccharide separation	100:120	[Preparation of core-shell silica-carbon composite microspheres stationary phase and application in saccharide separation].
34213249	1	75	theme	PF	320:321	arg1	polymer					323:329	the PF polymer	316:329	the PF polymer	316:329	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	1	76	theme	composite	175:183	arg1	MC					203:204	Sil@MC	199:204	Sil@MC	199:204	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	1	76	theme	composite	175:183	arg1	microspheres					185:196	core-shell mesoporous silica-carbon composite microspheres	139:196	core-shell mesoporous silica-carbon composite microspheres (Sil@MC)	139:205	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	6	77	theme	SiO2	863:866	arg1	calcination					848:858	calcination	848:858	calcination of SiO2 coated with phenolic resin	848:893	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	7	78	theme	phenolic	1187:1194	arg1	resin					1196:1200	phenolic resin	1187:1200	phenolic resin	1187:1200	However, the material formed by calcinating SiO2 without coating phenolic resin could not separate these polar sugar compounds by HPLC-MS.
34213249	8	79	dep	such	1402:1405	arg1	lacto-N-tetraose					1470:1485	lacto-N-tetraose	1470:1485	lacto-N-tetraose	1470:1485	Finally, the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose, were successfully separated by the Sil@MC column with good peak shapes.
34213249	6	80	contain	containing	1086:1095	arg1	acetonitrile-water					1066:1083	acetonitrile-water	1066:1083	acetonitrile-water (containing 0.1% (v/v) formic acid)	1066:1119	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	6	80	contain	containing	1086:1095	arg2	acid					1115:1118	0.1% (v/v) formic acid	1097:1118	0.1% (v/v) formic acid	1097:1118	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	8	81	with	column	1530:1535	arg1	shapes					1552:1557	good peak shapes	1542:1557	good peak shapes	1542:1557	Finally, the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose, were successfully separated by the Sil@MC column with good peak shapes.
34213249	4	82	theme	Sil	634:636	arg1	MC					638:639	the Sil@MC	630:639	the Sil@MC	630:639	The results showed that the Sil@MC was successfully immobilized on the silica particles via copolymerization and carbonization.
34213249	2	83	theme	morphology	362:371	arg1	observation					373:383	The morphology observation	358:383	The morphology observation of the Sil@MC stationary phase	358:414	The morphology observation of the Sil@MC stationary phase showed that it had good monodispersity.
34213249	0	84	theme	silica-carbon	27:39	arg1	microspheres					51:62	core-shell silica-carbon composite microspheres stationary phase and application	16:95	core-shell silica-carbon composite microspheres stationary phase and application	16:95	[Preparation of core-shell silica-carbon composite microspheres stationary phase and application in saccharide separation].
34213249	0	84	theme	silica-carbon	27:39	arg1	application					85:95	application	85:95	application	85:95	[Preparation of core-shell silica-carbon composite microspheres stationary phase and application in saccharide separation].
34213249	0	84	theme	silica-carbon	27:39	arg1	phase					75:79	stationary phase	64:79	stationary phase	64:79	[Preparation of core-shell silica-carbon composite microspheres stationary phase and application in saccharide separation].
34213249	2	85	theme	phase	410:414	arg1	observation					373:383	The morphology observation	358:383	The morphology observation of the Sil@MC stationary phase	358:414	The morphology observation of the Sil@MC stationary phase showed that it had good monodispersity.
34213249	3	86	theme	materials	549:557	arg1	nm					505:506	9.5 nm	501:506	9.5 nm	501:506	Surface area (302 m2/g), mean pore diameter (9.5 nm), and pore volume (0.63 cm3/g) of Sil@MC materials were also measured by pore structure analysis.
34213249	3	86	theme	materials	549:557	arg1	area					464:467	Surface area	456:467	Surface area (302 m2/g)	456:478	Surface area (302 m2/g), mean pore diameter (9.5 nm), and pore volume (0.63 cm3/g) of Sil@MC materials were also measured by pore structure analysis.
34213249	3	86	theme	materials	549:557	arg1	cm3/g					532:536	0.63 cm3/g	527:536	0.63 cm3/g	527:536	Surface area (302 m2/g), mean pore diameter (9.5 nm), and pore volume (0.63 cm3/g) of Sil@MC materials were also measured by pore structure analysis.
34213249	3	86	theme	materials	549:557	arg1	diameter					491:498	mean pore diameter	481:498	mean pore diameter (9.5 nm)	481:507	Surface area (302 m2/g), mean pore diameter (9.5 nm), and pore volume (0.63 cm3/g) of Sil@MC materials were also measured by pore structure analysis.
34213249	3	86	theme	materials	549:557	arg1	volume					519:524	pore volume	514:524	pore volume (0.63 cm3/g)	514:537	Surface area (302 m2/g), mean pore diameter (9.5 nm), and pore volume (0.63 cm3/g) of Sil@MC materials were also measured by pore structure analysis.
34213249	3	86	theme	materials	549:557	arg1	m2/g					474:477	302 m2/g	470:477	302 m2/g	470:477	Surface area (302 m2/g), mean pore diameter (9.5 nm), and pore volume (0.63 cm3/g) of Sil@MC materials were also measured by pore structure analysis.
34213249	2	87	theme	MC	396:397	arg1	phase					410:414	the Sil@MC stationary phase	388:414	the Sil@MC stationary phase	388:414	The morphology observation of the Sil@MC stationary phase showed that it had good monodispersity.
34213249	0	88	theme	microspheres	51:62	arg1	[Preparation					0:11	[Preparation	0:11	[Preparation of core-shell silica-carbon composite microspheres stationary phase and application in saccharide separation	0:120	[Preparation of core-shell silica-carbon composite microspheres stationary phase and application in saccharide separation].
34213249	1	89	theme	SiO2	284:287	arg1	surface					289:295	SiO2 surface	284:295	SiO2 surface	284:295	In this study, core-shell mesoporous silica-carbon composite microspheres (Sil@MC) were prepared by one-step coating of the phenol formaldehyde polymer (PF) on SiO2 surface and by carbonizing the PF polymer under nitrogen atmosphere.
34213249	9	90	theme	potential	1648:1656	arg1	application					1658:1668	potential application	1648:1668	potential application	1648:1668	The results demonstrates that silica-carbon composites derived from phenolic resin have potential application in polar compounds chromatographic separation.
34213249	8	91	theme	human	1366:1370	arg1	isomers					1393:1399	human milk oligosaccharide isomers	1366:1399	human milk oligosaccharide isomers	1366:1399	Finally, the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose, were successfully separated by the Sil@MC column with good peak shapes.
34213249	3	92	theme	@	545:545	arg1	materials					549:557	Sil@MC materials	542:557	Sil@MC materials	542:557	Surface area (302 m2/g), mean pore diameter (9.5 nm), and pore volume (0.63 cm3/g) of Sil@MC materials were also measured by pore structure analysis.
34213249	2	93	theme	Sil	392:394	arg1	phase					410:414	the Sil@MC stationary phase	388:414	the Sil@MC stationary phase	388:414	The morphology observation of the Sil@MC stationary phase showed that it had good monodispersity.
34213249	0	94	dep	microspheres	51:62	arg1	microspheres					51:62	core-shell silica-carbon composite microspheres stationary phase and application	16:95	core-shell silica-carbon composite microspheres stationary phase and application	16:95	[Preparation of core-shell silica-carbon composite microspheres stationary phase and application in saccharide separation].
34213249	0	94	dep	microspheres	51:62	arg1	application					85:95	application	85:95	application	85:95	[Preparation of core-shell silica-carbon composite microspheres stationary phase and application in saccharide separation].
34213249	0	94	dep	microspheres	51:62	arg1	phase					75:79	stationary phase	64:79	stationary phase	64:79	[Preparation of core-shell silica-carbon composite microspheres stationary phase and application in saccharide separation].
34213249	8	95	theme	oligosaccharide	1377:1391	arg1	isomers					1393:1399	human milk oligosaccharide isomers	1366:1399	human milk oligosaccharide isomers	1366:1399	Finally, the representative oligosaccharide isomers of raffinose, melezitose and stachyose, nystose, and human milk oligosaccharide isomers, such as 3'-sialyllactose, 6'-sialyllactose and lacto-N-newtetraose, lacto-N-tetraose, were successfully separated by the Sil@MC column with good peak shapes.
34213249	6	96	dep	compounds	948:956	arg1	dihydrat					1017:1024	D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat	959:1024	D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat	959:1024	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	6	96	dep	compounds	948:956	arg1	compounds					948:956	four polar sugar compounds	931:956	four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid)	931:1119	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	6	96	dep	compounds	948:956	arg1	raffinose					1030:1038	raffinose	1030:1038	raffinose	1030:1038	The Sil@MC materials formed after calcination of SiO2 coated with phenolic resin could be used for the separation of four polar sugar compounds (D-(+)-glucosamine hydrochloride, glucose, D-(+)-trehalose dihydrat and raffinose) with the mobile phase of acetonitrile-water (containing 0.1% (v/v) formic acid).
34213249	9	97	theme	compounds	1679:1687	arg1	separation					1705:1714	polar compounds chromatographic separation	1673:1714	polar compounds chromatographic separation	1673:1714	The results demonstrates that silica-carbon composites derived from phenolic resin have potential application in polar compounds chromatographic separation.
34213249	4	98	theme	silica	677:682	arg1	particles					684:692	the silica particles	673:692	the silica particles	673:692	The results showed that the Sil@MC was successfully immobilized on the silica particles via copolymerization and carbonization.
34213249	3	99	theme	mean	481:484	arg1	diameter					491:498	mean pore diameter	481:498	mean pore diameter (9.5 nm)	481:507	Surface area (302 m2/g), mean pore diameter (9.5 nm), and pore volume (0.63 cm3/g) of Sil@MC materials were also measured by pore structure analysis.
34213249	3	99	theme	mean	481:484	arg1	nm					505:506	9.5 nm	501:506	9.5 nm	501:506	Surface area (302 m2/g), mean pore diameter (9.5 nm), and pore volume (0.63 cm3/g) of Sil@MC materials were also measured by pore structure analysis.
34762318	5	0	contain	had	838:840	arg1	cellulase					763:771	cellulase	763:771	cellulase	763:771	The amount of cellulase had an important effect on the production of monosaccharides but had a smaller effect on the amounts and compositions of oligosaccharides.
34762318	5	0	contain	had	838:840	arg1	amount					753:758	The amount	749:758	The amount of cellulase	749:771	The amount of cellulase had an important effect on the production of monosaccharides but had a smaller effect on the amounts and compositions of oligosaccharides.
34762318	5	0	contain	had	838:840	arg2	effect					852:857	a smaller effect	842:857	a smaller effect	842:857	The amount of cellulase had an important effect on the production of monosaccharides but had a smaller effect on the amounts and compositions of oligosaccharides.
34762318	4	1	theme	Ethanol	641:647	arg1	precipitation					649:661	Ethanol precipitation	641:661	Ethanol precipitation followed by analysis	641:682	Ethanol precipitation followed by analysis showed that there were many oligosaccharides in the hydrolysate.
34762318	0	2	theme	protein	82:88	arg1	production					56:65	the production	52:65	the production of single-cell protein with Saccharomyces cerevisiae	52:118	Oligosaccharides in straw hydrolysate could improve the production of single-cell protein with Saccharomyces cerevisiae.
34762318	3	3	theme	yeast	627:631	arg1	growth					633:638	yeast growth	627:638	yeast growth	627:638	RESULTS At the same glucose concentration, the dry weight of cells produced in the hydrolysate was 2.89 times higher than that in 2 g L-1 yeast extract (YE) medium, indicating that the hydrolysate was a suitable substrate for yeast growth.
34762318	9	4	from	Oligosaccharides	1309:1324	arg1	hydrolysate					1333:1343	the hydrolysate	1329:1343	the hydrolysate	1329:1343	CONCLUSION Oligosaccharides in the hydrolysate can improve SCP production with low nutrient cost.
34762318	5	5	theme	smaller	844:850	arg1	effect					852:857	a smaller effect	842:857	a smaller effect	842:857	The amount of cellulase had an important effect on the production of monosaccharides but had a smaller effect on the amounts and compositions of oligosaccharides.
34762318	8	6	with	cost	1192:1195	arg1	hydrolysate					1220:1230	the hydrolysate	1216:1230	the hydrolysate	1216:1230	Compared with YE medium, the cost of the medium with the hydrolysate was reduced by 68.47% when the same dry cell weight was obtained.
34762318	0	7	from	Oligosaccharides	0:15	arg1	hydrolysate					26:36	straw hydrolysate	20:36	straw hydrolysate	20:36	Oligosaccharides in straw hydrolysate could improve the production of single-cell protein with Saccharomyces cerevisiae.
34762318	2	8	theme	enzyme	295:300	arg1	loading					302:308	enzyme loading	295:308	enzyme loading	295:308	The aims of this study were to investigate the effects of enzyme loading on the components of rice straw (RS) hydrolysate and their effects on the growth of yeast.
34762318	0	9	theme	Saccharomyces	95:107	arg1	cerevisiae					109:118	Saccharomyces cerevisiae	95:118	Saccharomyces cerevisiae	95:118	Oligosaccharides in straw hydrolysate could improve the production of single-cell protein with Saccharomyces cerevisiae.
34762318	7	10	from	factor	1126:1131	arg1	hydrolysate					1150:1160	the hydrolysate	1146:1160	the hydrolysate	1146:1160	The results indicate that oligosaccharides were an important growth factor for yeast in the hydrolysate.
34762318	8	11	theme	dry	1268:1270	arg1	weight					1277:1282	the same dry cell weight	1259:1282	the same dry cell weight	1259:1282	Compared with YE medium, the cost of the medium with the hydrolysate was reduced by 68.47% when the same dry cell weight was obtained.
34762318	4	12	from	oligosaccharides	712:727	arg1	hydrolysate					736:746	the hydrolysate	732:746	the hydrolysate	732:746	Ethanol precipitation followed by analysis showed that there were many oligosaccharides in the hydrolysate.
34762318	4	13	theme	many	707:710	arg1	oligosaccharides					712:727	many oligosaccharides	707:727	many oligosaccharides in the hydrolysate	707:746	Ethanol precipitation followed by analysis showed that there were many oligosaccharides in the hydrolysate.
34762318	8	14	theme	medium	1204:1209	arg1	cost					1192:1195	the cost	1188:1195	the cost of the medium with the hydrolysate	1188:1230	Compared with YE medium, the cost of the medium with the hydrolysate was reduced by 68.47% when the same dry cell weight was obtained.
34762318	6	15	theme	SCP	1030:1032	arg1	production					1034:1043	SCP production	1030:1043	SCP production	1030:1043	Adding oligosaccharides to the medium had no effect on ethanol production, but it promoted yeast growth and increased SCP production effectively.
34762318	8	16	theme	YE	1177:1178	arg1	medium					1180:1185	YE medium	1177:1185	YE medium	1177:1185	Compared with YE medium, the cost of the medium with the hydrolysate was reduced by 68.47% when the same dry cell weight was obtained.
34762318	10	17	theme	SCP	1546:1548	arg1	production					1550:1559	SCP production	1546:1559	SCP production	1546:1559	This finding could reduce the amounts of cellulase required during saccharification and nutrients during culture, providing a new low-cost method for SCP production.
34762318	3	18	theme	same	416:419	arg1	concentration					429:441	the same glucose concentration	412:441	the same glucose concentration	412:441	RESULTS At the same glucose concentration, the dry weight of cells produced in the hydrolysate was 2.89 times higher than that in 2 g L-1 yeast extract (YE) medium, indicating that the hydrolysate was a suitable substrate for yeast growth.
34762318	6	19	theme	Adding	912:917	arg1	oligosaccharides					919:934	Adding oligosaccharides	912:934	Adding oligosaccharides to the medium	912:948	Adding oligosaccharides to the medium had no effect on ethanol production, but it promoted yeast growth and increased SCP production effectively.
34762318	5	20	theme	monosaccharides	818:832	arg1	production					804:813	the production	800:813	the production of monosaccharides	800:832	The amount of cellulase had an important effect on the production of monosaccharides but had a smaller effect on the amounts and compositions of oligosaccharides.
34762318	5	21	theme	cellulase	763:771	arg1	cellulase					763:771	cellulase	763:771	cellulase	763:771	The amount of cellulase had an important effect on the production of monosaccharides but had a smaller effect on the amounts and compositions of oligosaccharides.
34762318	5	21	theme	cellulase	763:771	arg1	amount					753:758	The amount	749:758	The amount of cellulase	749:771	The amount of cellulase had an important effect on the production of monosaccharides but had a smaller effect on the amounts and compositions of oligosaccharides.
34762318	6	22	theme	yeast	1003:1007	arg1	growth					1009:1014	yeast growth	1003:1014	yeast growth	1003:1014	Adding oligosaccharides to the medium had no effect on ethanol production, but it promoted yeast growth and increased SCP production effectively.
34762318	3	23	theme	2 g L-1	531:537	arg1	YE					554:555	YE	554:555	YE	554:555	RESULTS At the same glucose concentration, the dry weight of cells produced in the hydrolysate was 2.89 times higher than that in 2 g L-1 yeast extract (YE) medium, indicating that the hydrolysate was a suitable substrate for yeast growth.
34762318	3	23	theme	2 g L-1	531:537	arg1	extract					545:551	2 g L-1 yeast extract	531:551	2 g L-1 yeast extract (YE) medium	531:563	RESULTS At the same glucose concentration, the dry weight of cells produced in the hydrolysate was 2.89 times higher than that in 2 g L-1 yeast extract (YE) medium, indicating that the hydrolysate was a suitable substrate for yeast growth.
34762318	10	24	theme	cellulase	1437:1445	arg1	cellulase					1437:1445	cellulase	1437:1445	cellulase required during saccharification and nutrients	1437:1492	This finding could reduce the amounts of cellulase required during saccharification and nutrients during culture, providing a new low-cost method for SCP production.
34762318	10	24	theme	cellulase	1437:1445	arg1	amounts					1426:1432	the amounts	1422:1432	the amounts of cellulase required during saccharification and nutrients	1422:1492	This finding could reduce the amounts of cellulase required during saccharification and nutrients during culture, providing a new low-cost method for SCP production.
34762318	9	25	theme	low	1377:1379	arg1	cost					1390:1393	low nutrient cost	1377:1393	low nutrient cost	1377:1393	CONCLUSION Oligosaccharides in the hydrolysate can improve SCP production with low nutrient cost.
34762318	5	26	theme	oligosaccharides	894:909	arg1	oligosaccharides					894:909	oligosaccharides	894:909	oligosaccharides	894:909	The amount of cellulase had an important effect on the production of monosaccharides but had a smaller effect on the amounts and compositions of oligosaccharides.
34762318	5	26	theme	oligosaccharides	894:909	arg1	compositions					878:889	compositions	878:889	compositions	878:889	The amount of cellulase had an important effect on the production of monosaccharides but had a smaller effect on the amounts and compositions of oligosaccharides.
34762318	5	26	theme	oligosaccharides	894:909	arg1	amounts					866:872	amounts	866:872	amounts	866:872	The amount of cellulase had an important effect on the production of monosaccharides but had a smaller effect on the amounts and compositions of oligosaccharides.
34762318	1	27	theme	production	207:216	arg1	costs					218:222	production costs	207:222	production costs	207:222	BACKGROUND Using agricultural wastes to produce single-cell proteins (SCP) can reduce production costs effectively.
34762318	0	28	theme	straw	20:24	arg1	hydrolysate					26:36	straw hydrolysate	20:36	straw hydrolysate	20:36	Oligosaccharides in straw hydrolysate could improve the production of single-cell protein with Saccharomyces cerevisiae.
34762318	2	29	theme	loading	302:308	arg1	effects					284:290	the effects	280:290	the effects of enzyme loading on the components of rice straw (RS) hydrolysate	280:357	The aims of this study were to investigate the effects of enzyme loading on the components of rice straw (RS) hydrolysate and their effects on the growth of yeast.
34762318	2	29	theme	loading	302:308	arg1	effects					369:375	their effects	363:375	their effects on the growth of yeast	363:398	The aims of this study were to investigate the effects of enzyme loading on the components of rice straw (RS) hydrolysate and their effects on the growth of yeast.
34762318	2	30	theme	yeast	394:398	arg1	growth					384:389	the growth	380:389	the growth of yeast	380:398	The aims of this study were to investigate the effects of enzyme loading on the components of rice straw (RS) hydrolysate and their effects on the growth of yeast.
34762318	7	31	theme	important	1109:1117	arg1	factor					1126:1131	an important growth factor	1106:1131	an important growth factor for yeast in the hydrolysate	1106:1160	The results indicate that oligosaccharides were an important growth factor for yeast in the hydrolysate.
34762318	7	31	theme	important	1109:1117	arg1	oligosaccharides					1084:1099	oligosaccharides	1084:1099	oligosaccharides	1084:1099	The results indicate that oligosaccharides were an important growth factor for yeast in the hydrolysate.
34762318	2	32	theme	straw	336:340	arg1	hydrolysate					347:357	rice straw (RS) hydrolysate	331:357	rice straw (RS) hydrolysate	331:357	The aims of this study were to investigate the effects of enzyme loading on the components of rice straw (RS) hydrolysate and their effects on the growth of yeast.
34762318	5	33	contain	had	773:775	arg1	cellulase					763:771	cellulase	763:771	cellulase	763:771	The amount of cellulase had an important effect on the production of monosaccharides but had a smaller effect on the amounts and compositions of oligosaccharides.
34762318	5	33	contain	had	773:775	arg1	amount					753:758	The amount	749:758	The amount of cellulase	749:771	The amount of cellulase had an important effect on the production of monosaccharides but had a smaller effect on the amounts and compositions of oligosaccharides.
34762318	5	33	contain	had	773:775	arg2	effect					790:795	an important effect	777:795	an important effect	777:795	The amount of cellulase had an important effect on the production of monosaccharides but had a smaller effect on the amounts and compositions of oligosaccharides.
34762318	3	34	dep	RESULTS	401:407	arg1	higher					511:516	higher	511:516	higher	511:516	RESULTS At the same glucose concentration, the dry weight of cells produced in the hydrolysate was 2.89 times higher than that in 2 g L-1 yeast extract (YE) medium, indicating that the hydrolysate was a suitable substrate for yeast growth.
34762318	3	34	dep	RESULTS	401:407	arg1	weight					452:457	the dry weight	444:457	the dry weight of cells produced in the hydrolysate	444:494	RESULTS At the same glucose concentration, the dry weight of cells produced in the hydrolysate was 2.89 times higher than that in 2 g L-1 yeast extract (YE) medium, indicating that the hydrolysate was a suitable substrate for yeast growth.
34762318	3	35	theme	glucose	421:427	arg1	concentration					429:441	the same glucose concentration	412:441	the same glucose concentration	412:441	RESULTS At the same glucose concentration, the dry weight of cells produced in the hydrolysate was 2.89 times higher than that in 2 g L-1 yeast extract (YE) medium, indicating that the hydrolysate was a suitable substrate for yeast growth.
34762318	7	36	theme	growth	1119:1124	arg1	factor					1126:1131	an important growth factor	1106:1131	an important growth factor for yeast in the hydrolysate	1106:1160	The results indicate that oligosaccharides were an important growth factor for yeast in the hydrolysate.
34762318	7	36	theme	growth	1119:1124	arg1	oligosaccharides					1084:1099	oligosaccharides	1084:1099	oligosaccharides	1084:1099	The results indicate that oligosaccharides were an important growth factor for yeast in the hydrolysate.
34762318	9	37	theme	SCP	1357:1359	arg1	production					1361:1370	SCP production	1357:1370	SCP production	1357:1370	CONCLUSION Oligosaccharides in the hydrolysate can improve SCP production with low nutrient cost.
34762318	11	38	theme	Chemical	1580:1587	arg1	Industry					1589:1596	Chemical Industry	1580:1596	Chemical Industry	1580:1596	© 2021 Society of Chemical Industry.
34762318	2	39	from	effects	369:375	arg1	growth					384:389	the growth	380:389	the growth of yeast	380:398	The aims of this study were to investigate the effects of enzyme loading on the components of rice straw (RS) hydrolysate and their effects on the growth of yeast.
34762318	2	39	from	effects	369:375	arg1	components					317:326	the components	313:326	the components of rice straw (RS) hydrolysate	313:357	The aims of this study were to investigate the effects of enzyme loading on the components of rice straw (RS) hydrolysate and their effects on the growth of yeast.
34762318	8	40	theme	cell	1272:1275	arg1	weight					1277:1282	the same dry cell weight	1259:1282	the same dry cell weight	1259:1282	Compared with YE medium, the cost of the medium with the hydrolysate was reduced by 68.47% when the same dry cell weight was obtained.
34762318	3	41	theme	yeast	539:543	arg1	YE					554:555	YE	554:555	YE	554:555	RESULTS At the same glucose concentration, the dry weight of cells produced in the hydrolysate was 2.89 times higher than that in 2 g L-1 yeast extract (YE) medium, indicating that the hydrolysate was a suitable substrate for yeast growth.
34762318	3	41	theme	yeast	539:543	arg1	extract					545:551	2 g L-1 yeast extract	531:551	2 g L-1 yeast extract (YE) medium	531:563	RESULTS At the same glucose concentration, the dry weight of cells produced in the hydrolysate was 2.89 times higher than that in 2 g L-1 yeast extract (YE) medium, indicating that the hydrolysate was a suitable substrate for yeast growth.
34762318	6	42	contain	had	950:952	arg1	oligosaccharides					919:934	Adding oligosaccharides	912:934	Adding oligosaccharides to the medium	912:948	Adding oligosaccharides to the medium had no effect on ethanol production, but it promoted yeast growth and increased SCP production effectively.
34762318	6	42	contain	had	950:952	arg2	effect					957:962	no effect	954:962	no effect	954:962	Adding oligosaccharides to the medium had no effect on ethanol production, but it promoted yeast growth and increased SCP production effectively.
34762318	10	43	dep	new	1522:1524	arg1	low-cost					1526:1533	low-cost	1526:1533	low-cost	1526:1533	This finding could reduce the amounts of cellulase required during saccharification and nutrients during culture, providing a new low-cost method for SCP production.
34762318	9	44	theme	CONCLUSION	1298:1307	arg1	Oligosaccharides					1309:1324	CONCLUSION Oligosaccharides	1298:1324	CONCLUSION Oligosaccharides in the hydrolysate	1298:1343	CONCLUSION Oligosaccharides in the hydrolysate can improve SCP production with low nutrient cost.
34762318	3	45	theme	extract	545:551	arg1	medium					558:563	2 g L-1 yeast extract (YE) medium	531:563	2 g L-1 yeast extract (YE) medium	531:563	RESULTS At the same glucose concentration, the dry weight of cells produced in the hydrolysate was 2.89 times higher than that in 2 g L-1 yeast extract (YE) medium, indicating that the hydrolysate was a suitable substrate for yeast growth.
34762318	1	46	theme	agricultural	138:149	arg1	wastes					151:156	agricultural wastes	138:156	agricultural wastes	138:156	BACKGROUND Using agricultural wastes to produce single-cell proteins (SCP) can reduce production costs effectively.
34762318	2	47	from	effects	284:290	arg1	growth					384:389	the growth	380:389	the growth of yeast	380:398	The aims of this study were to investigate the effects of enzyme loading on the components of rice straw (RS) hydrolysate and their effects on the growth of yeast.
34762318	2	47	from	effects	284:290	arg1	components					317:326	the components	313:326	the components of rice straw (RS) hydrolysate	313:357	The aims of this study were to investigate the effects of enzyme loading on the components of rice straw (RS) hydrolysate and their effects on the growth of yeast.
34762318	2	48	theme	rice	331:334	arg1	straw					336:340	rice straw	331:340	rice straw (RS) hydrolysate	331:357	The aims of this study were to investigate the effects of enzyme loading on the components of rice straw (RS) hydrolysate and their effects on the growth of yeast.
34762318	2	48	theme	rice	331:334	arg1	RS					343:344	RS	343:344	RS	343:344	The aims of this study were to investigate the effects of enzyme loading on the components of rice straw (RS) hydrolysate and their effects on the growth of yeast.
34762318	5	49	dep	amounts	866:872	arg1	the					862:864	the	862:864	the	862:864	The amount of cellulase had an important effect on the production of monosaccharides but had a smaller effect on the amounts and compositions of oligosaccharides.
34762318	0	50	with	production	56:65	arg1	cerevisiae					109:118	Saccharomyces cerevisiae	95:118	Saccharomyces cerevisiae	95:118	Oligosaccharides in straw hydrolysate could improve the production of single-cell protein with Saccharomyces cerevisiae.
34762318	1	51	theme	single-cell	169:179	arg1	SCP					191:193	SCP	191:193	SCP	191:193	BACKGROUND Using agricultural wastes to produce single-cell proteins (SCP) can reduce production costs effectively.
34762318	1	51	theme	single-cell	169:179	arg1	proteins					181:188	single-cell proteins	169:188	single-cell proteins (SCP)	169:194	BACKGROUND Using agricultural wastes to produce single-cell proteins (SCP) can reduce production costs effectively.
34762318	8	52	theme	same	1263:1266	arg1	weight					1277:1282	the same dry cell weight	1259:1282	the same dry cell weight	1259:1282	Compared with YE medium, the cost of the medium with the hydrolysate was reduced by 68.47% when the same dry cell weight was obtained.
34762318	3	53	theme	dry	448:450	arg1	higher					511:516	higher	511:516	higher	511:516	RESULTS At the same glucose concentration, the dry weight of cells produced in the hydrolysate was 2.89 times higher than that in 2 g L-1 yeast extract (YE) medium, indicating that the hydrolysate was a suitable substrate for yeast growth.
34762318	3	53	theme	dry	448:450	arg1	weight					452:457	the dry weight	444:457	the dry weight of cells produced in the hydrolysate	444:494	RESULTS At the same glucose concentration, the dry weight of cells produced in the hydrolysate was 2.89 times higher than that in 2 g L-1 yeast extract (YE) medium, indicating that the hydrolysate was a suitable substrate for yeast growth.
34762318	0	54	theme	single-cell	70:80	arg1	protein					82:88	single-cell protein	70:88	single-cell protein	70:88	Oligosaccharides in straw hydrolysate could improve the production of single-cell protein with Saccharomyces cerevisiae.
34762318	3	55	theme	suitable	604:611	arg1	hydrolysate					586:596	the hydrolysate	582:596	the hydrolysate	582:596	RESULTS At the same glucose concentration, the dry weight of cells produced in the hydrolysate was 2.89 times higher than that in 2 g L-1 yeast extract (YE) medium, indicating that the hydrolysate was a suitable substrate for yeast growth.
34762318	3	55	theme	suitable	604:611	arg1	substrate					613:621	a suitable substrate	602:621	a suitable substrate for yeast growth	602:638	RESULTS At the same glucose concentration, the dry weight of cells produced in the hydrolysate was 2.89 times higher than that in 2 g L-1 yeast extract (YE) medium, indicating that the hydrolysate was a suitable substrate for yeast growth.
34762318	5	56	theme	important	780:788	arg1	effect					790:795	an important effect	777:795	an important effect	777:795	The amount of cellulase had an important effect on the production of monosaccharides but had a smaller effect on the amounts and compositions of oligosaccharides.
34762318	9	57	theme	nutrient	1381:1388	arg1	cost					1390:1393	low nutrient cost	1377:1393	low nutrient cost	1377:1393	CONCLUSION Oligosaccharides in the hydrolysate can improve SCP production with low nutrient cost.
34762318	6	58	theme	ethanol	967:973	arg1	production					975:984	ethanol production	967:984	ethanol production	967:984	Adding oligosaccharides to the medium had no effect on ethanol production, but it promoted yeast growth and increased SCP production effectively.
34762318	2	59	theme	study	254:258	arg1	aims					241:244	The aims	237:244	The aims of this study	237:258	The aims of this study were to investigate the effects of enzyme loading on the components of rice straw (RS) hydrolysate and their effects on the growth of yeast.
34762318	2	60	theme	hydrolysate	347:357	arg1	components					317:326	the components	313:326	the components of rice straw (RS) hydrolysate	313:357	The aims of this study were to investigate the effects of enzyme loading on the components of rice straw (RS) hydrolysate and their effects on the growth of yeast.
34762318	3	61	theme	cells	462:466	arg1	higher					511:516	higher	511:516	higher	511:516	RESULTS At the same glucose concentration, the dry weight of cells produced in the hydrolysate was 2.89 times higher than that in 2 g L-1 yeast extract (YE) medium, indicating that the hydrolysate was a suitable substrate for yeast growth.
34762318	3	61	theme	cells	462:466	arg1	weight					452:457	the dry weight	444:457	the dry weight of cells produced in the hydrolysate	444:494	RESULTS At the same glucose concentration, the dry weight of cells produced in the hydrolysate was 2.89 times higher than that in 2 g L-1 yeast extract (YE) medium, indicating that the hydrolysate was a suitable substrate for yeast growth.
34762318	10	62	theme	new	1522:1524	arg1	method					1535:1540	a new low-cost method	1520:1540	a new low-cost method for SCP production	1520:1559	This finding could reduce the amounts of cellulase required during saccharification and nutrients during culture, providing a new low-cost method for SCP production.
34807566	9	0	theme	complex	1767:1773	arg1	mixtures					1789:1796	complex bacterial LPS mixtures	1767:1796	complex bacterial LPS mixtures	1767:1796	Our results demonstrate that nLC-ESI-FT-MS is an attractive strategy for the structural profiling of small quantities of complex bacterial LPS mixtures in their intact form.
34807566	5	1	dep	serotype	1137:1144	arg1	Typhimurium					1146:1156	Salmonella enterica serotype Typhimurium	1117:1156	Salmonella enterica serotype Typhimurium	1117:1156	Furthermore, the method enabled the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4.
34807566	3	2	theme	liquid	750:755	arg1	nLC					773:775	nLC	773:775	nLC	773:775	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	3	2	theme	liquid	750:755	arg1	chromatography					757:770	nanoflow reversed-phase high-performance liquid chromatography	709:770	nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS)	709:859	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	9	3	theme	LPS	1785:1787	arg1	mixtures					1789:1796	complex bacterial LPS mixtures	1767:1796	complex bacterial LPS mixtures	1767:1796	Our results demonstrate that nLC-ESI-FT-MS is an attractive strategy for the structural profiling of small quantities of complex bacterial LPS mixtures in their intact form.
34807566	7	4	theme	single	1532:1537	arg1	species					1550:1556	single S-type LPS species	1532:1556	single S-type LPS species	1532:1556	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	6	5	theme	various	1261:1267	arg1	lengths					1269:1275	various lengths	1261:1275	various lengths of the O-specific polysaccharide	1261:1308	High-resolution, accurate mass spectra of intact LPS containing various lengths of the O-specific polysaccharide in the range of 3 and 15 kDa were obtained.
34807566	6	6	theme	kDa	1335:1337	arg1	range					1317:1321	the range	1313:1321	the range of 3 and 15 kDa	1313:1337	High-resolution, accurate mass spectra of intact LPS containing various lengths of the O-specific polysaccharide in the range of 3 and 15 kDa were obtained.
34807566	7	7	theme	species	1550:1556	arg1	oligo/polysaccharides					1487:1507	oligo/polysaccharides	1487:1507	oligo/polysaccharides	1487:1507	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	7	7	theme	species	1550:1556	arg1	composition					1472:1482	the composition	1468:1482	the composition of oligo/polysaccharides	1468:1507	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	7	7	theme	species	1550:1556	arg1	species					1550:1556	single S-type LPS species	1532:1556	single S-type LPS species	1532:1556	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	7	7	theme	species	1550:1556	arg1	domains					1521:1527	lipid A domains	1513:1527	lipid A domains	1513:1527	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	9	8	theme	intact	1807:1812	arg1	form					1814:1817	their intact form	1801:1817	their intact form	1801:1817	Our results demonstrate that nLC-ESI-FT-MS is an attractive strategy for the structural profiling of small quantities of complex bacterial LPS mixtures in their intact form.
34807566	6	9	theme	LPS	1246:1248	arg1	spectra					1228:1234	High-resolution, accurate mass spectra	1197:1234	High-resolution, accurate mass spectra of intact LPS containing various lengths of the O-specific polysaccharide in the range of 3 and 15 kDa	1197:1337	High-resolution, accurate mass spectra of intact LPS containing various lengths of the O-specific polysaccharide in the range of 3 and 15 kDa were obtained.
34807566	7	10	theme	oligo/polysaccharides	1487:1507	arg1	oligo/polysaccharides					1487:1507	oligo/polysaccharides	1487:1507	oligo/polysaccharides	1487:1507	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	7	10	theme	oligo/polysaccharides	1487:1507	arg1	composition					1472:1482	the composition	1468:1482	the composition of oligo/polysaccharides	1468:1507	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	7	10	theme	oligo/polysaccharides	1487:1507	arg1	species					1550:1556	single S-type LPS species	1532:1556	single S-type LPS species	1532:1556	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	7	10	theme	oligo/polysaccharides	1487:1507	arg1	domains					1521:1527	lipid A domains	1513:1527	lipid A domains	1513:1527	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	3	11	theme	ionization	802:811	arg1	ESI-FT-MS					850:858	ESI-FT-MS	850:858	ESI-FT-MS	850:858	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	3	11	theme	ionization	802:811	arg1	spectrometry					836:847	electrospray ionization Fourier transform mass spectrometry	789:847	electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS)	789:859	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	8	12	gly	heterogeneity	1574:1586	arg1	LPS					1598:1600	S-type LPS	1591:1600	S-type LPS	1591:1600	The structural heterogeneity of S-type LPS was characterized by unprecedented details.
34807566	5	13	theme	Salmonella	1117:1126	arg1	serotype					1137:1144	Salmonella enterica serotype Typhimurium	1117:1156	Salmonella enterica serotype Typhimurium	1117:1156	Furthermore, the method enabled the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4.
34807566	8	14	theme	structural	1563:1572	arg1	heterogeneity					1574:1586	The structural heterogeneity	1559:1586	The structural heterogeneity of S-type LPS	1559:1600	The structural heterogeneity of S-type LPS was characterized by unprecedented details.
34807566	7	15	theme	detailed	1444:1451	arg1	information					1453:1463	detailed information	1444:1463	detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species	1444:1556	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	3	16	theme	complex	672:678	arg1	mixtures					691:698	complex intact LPS mixtures	672:698	complex intact LPS mixtures	672:698	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	1	17	theme	outer	157:161	arg1	membrane					163:170	the outer membrane lipopolysaccharide (LPS)	153:195	the outer membrane lipopolysaccharide (LPS)	153:195	The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria contributes to bacterial pathogenesis through various mechanisms, including the development of antibiotic resistance and evasion of the immune response of the host.
34807566	6	18	theme	O-specific	1284:1293	arg1	polysaccharide					1295:1308	the O-specific polysaccharide	1280:1308	the O-specific polysaccharide	1280:1308	High-resolution, accurate mass spectra of intact LPS containing various lengths of the O-specific polysaccharide in the range of 3 and 15 kDa were obtained.
34807566	0	19	theme	Heterogeneity	68:80	arg1	Characterization					40:55	In-Depth Characterization	31:55	In-Depth Characterization of Natural Heterogeneity of Complex Bacterial Lipopolysaccharides	31:121	Nanoflow LC-MS Method Allowing In-Depth Characterization of Natural Heterogeneity of Complex Bacterial Lipopolysaccharides.
34807566	8	20	theme	LPS	1598:1600	arg1	heterogeneity					1574:1586	The structural heterogeneity	1559:1586	The structural heterogeneity of S-type LPS	1559:1600	The structural heterogeneity of S-type LPS was characterized by unprecedented details.
34807566	5	21	dep	analysis	1023:1030	arg1	B4					1193:1194	B4	1193:1194	the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4	1019:1194	Furthermore, the method enabled the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4.
34807566	0	22	theme	Complex	85:91	arg1	Lipopolysaccharides					103:121	Complex Bacterial Lipopolysaccharides	85:121	Complex Bacterial Lipopolysaccharides	85:121	Nanoflow LC-MS Method Allowing In-Depth Characterization of Natural Heterogeneity of Complex Bacterial Lipopolysaccharides.
34807566	1	23	theme	host	382:385	arg1	response					366:373	the immune response	355:373	the immune response of the host	355:385	The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria contributes to bacterial pathogenesis through various mechanisms, including the development of antibiotic resistance and evasion of the immune response of the host.
34807566	1	24	theme	variable	128:135	arg1	modification					137:148	The variable modification	124:148	The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria	124:221	The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria contributes to bacterial pathogenesis through various mechanisms, including the development of antibiotic resistance and evasion of the immune response of the host.
34807566	0	25	theme	Lipopolysaccharides	103:121	arg1	Heterogeneity					68:80	Natural Heterogeneity	60:80	Natural Heterogeneity of Complex Bacterial Lipopolysaccharides	60:121	Nanoflow LC-MS Method Allowing In-Depth Characterization of Natural Heterogeneity of Complex Bacterial Lipopolysaccharides.
34807566	3	26	dep	spectrometry	836:847	arg1	transform					821:829	transform	821:829	transform	821:829	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	8	27	theme	unprecedented	1623:1635	arg1	details					1637:1643	unprecedented details	1623:1643	unprecedented details	1623:1643	The structural heterogeneity of S-type LPS was characterized by unprecedented details.
34807566	4	28	from	meningitidis	924:935	arg1	quantities					871:880	Nanogram quantities	862:880	Nanogram quantities of rough-type LPS mixtures from Neisseria meningitidis	862:935	Nanogram quantities of rough-type LPS mixtures from Neisseria meningitidis could be separated and analyzed by nLC-ESI-FT-MS.
34807566	4	28	from	meningitidis	924:935	arg1	mixtures					900:907	rough-type LPS mixtures	885:907	rough-type LPS mixtures from Neisseria meningitidis	885:935	Nanogram quantities of rough-type LPS mixtures from Neisseria meningitidis could be separated and analyzed by nLC-ESI-FT-MS.
34807566	2	29	gly	heterogeneity	475:487	arg1	glycolipids					555:565	these glycolipids	549:565	these glycolipids	549:565	Characterizing the natural structural repertoire of LPS is challenging due to the high heterogeneity, branched architecture, and strong amphipathic character of these glycolipids.
34807566	7	30	theme	intact	1424:1429	arg1	LPS					1431:1433	intact LPS	1424:1433	intact LPS	1424:1433	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	2	31	theme	branched	490:497	arg1	architecture					499:510	branched architecture	490:510	branched architecture	490:510	Characterizing the natural structural repertoire of LPS is challenging due to the high heterogeneity, branched architecture, and strong amphipathic character of these glycolipids.
34807566	0	32	theme	Nanoflow	0:7	arg1	Method					15:20	Nanoflow LC-MS Method	0:20	Nanoflow LC-MS Method	0:20	Nanoflow LC-MS Method Allowing In-Depth Characterization of Natural Heterogeneity of Complex Bacterial Lipopolysaccharides.
34807566	1	33	theme	antibiotic	318:327	arg1	resistance					329:338	antibiotic resistance	318:338	antibiotic resistance	318:338	The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria contributes to bacterial pathogenesis through various mechanisms, including the development of antibiotic resistance and evasion of the immune response of the host.
34807566	1	34	from	modification	137:148	arg1	bacteria					214:221	Gram-negative bacteria	200:221	Gram-negative bacteria	200:221	The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria contributes to bacterial pathogenesis through various mechanisms, including the development of antibiotic resistance and evasion of the immune response of the host.
34807566	7	35	theme	LPS	1431:1433	arg1	dissociation					1408:1419	collision-induced dissociation	1390:1419	collision-induced dissociation of intact LPS	1390:1433	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	1	36	dep	membrane	163:170	arg1	lipopolysaccharide					172:189	lipopolysaccharide	172:189	the outer membrane lipopolysaccharide (LPS)	153:195	The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria contributes to bacterial pathogenesis through various mechanisms, including the development of antibiotic resistance and evasion of the immune response of the host.
34807566	1	36	dep	membrane	163:170	arg1	LPS					192:194	LPS	192:194	LPS	192:194	The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria contributes to bacterial pathogenesis through various mechanisms, including the development of antibiotic resistance and evasion of the immune response of the host.
34807566	5	37	theme	heterogeneous	1042:1054	arg1	LPS					1072:1074	highly heterogeneous smooth (S)-type LPS	1035:1074	highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium	1035:1156	Furthermore, the method enabled the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4.
34807566	2	38	theme	strong	517:522	arg1	character					536:544	strong amphipathic character	517:544	strong amphipathic character	517:544	Characterizing the natural structural repertoire of LPS is challenging due to the high heterogeneity, branched architecture, and strong amphipathic character of these glycolipids.
34807566	4	39	theme	LPS	896:898	arg1	mixtures					900:907	rough-type LPS mixtures	885:907	rough-type LPS mixtures from Neisseria meningitidis	885:935	Nanogram quantities of rough-type LPS mixtures from Neisseria meningitidis could be separated and analyzed by nLC-ESI-FT-MS.
34807566	0	40	theme	In-Depth	31:38	arg1	Characterization					40:55	In-Depth Characterization	31:55	In-Depth Characterization of Natural Heterogeneity of Complex Bacterial Lipopolysaccharides	31:121	Nanoflow LC-MS Method Allowing In-Depth Characterization of Natural Heterogeneity of Complex Bacterial Lipopolysaccharides.
34807566	7	41	theme	MS/MS	1367:1371	arg1	experiments					1373:1383	MS/MS experiments	1367:1383	MS/MS experiments with collision-induced dissociation of intact LPS	1367:1433	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	3	42	theme	LPS	687:689	arg1	mixtures					691:698	complex intact LPS mixtures	672:698	complex intact LPS mixtures	672:698	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	1	43	theme	immune	359:364	arg1	response					366:373	the immune response	355:373	the immune response of the host	355:385	The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria contributes to bacterial pathogenesis through various mechanisms, including the development of antibiotic resistance and evasion of the immune response of the host.
34807566	3	44	theme	high-performance	733:748	arg1	nLC					773:775	nLC	773:775	nLC	773:775	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	3	44	theme	high-performance	733:748	arg1	chromatography					757:770	nanoflow reversed-phase high-performance liquid chromatography	709:770	nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS)	709:859	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	4	45	theme	Nanogram	862:869	arg1	quantities					871:880	Nanogram quantities	862:880	Nanogram quantities of rough-type LPS mixtures from Neisseria meningitidis	862:935	Nanogram quantities of rough-type LPS mixtures from Neisseria meningitidis could be separated and analyzed by nLC-ESI-FT-MS.
34807566	5	46	theme	LPS	1072:1074	arg1	analysis					1023:1030	the analysis	1019:1030	the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4	1019:1194	Furthermore, the method enabled the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4.
34807566	6	47	theme	High-resolution	1197:1211	arg1	spectra					1228:1234	High-resolution, accurate mass spectra	1197:1234	High-resolution, accurate mass spectra of intact LPS containing various lengths of the O-specific polysaccharide in the range of 3 and 15 kDa	1197:1337	High-resolution, accurate mass spectra of intact LPS containing various lengths of the O-specific polysaccharide in the range of 3 and 15 kDa were obtained.
34807566	3	48	theme	nanoflow	709:716	arg1	nLC					773:775	nLC	773:775	nLC	773:775	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	3	48	theme	nanoflow	709:716	arg1	chromatography					757:770	nanoflow reversed-phase high-performance liquid chromatography	709:770	nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS)	709:859	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	2	49	theme	structural	415:424	arg1	LPS					440:442	LPS	440:442	LPS	440:442	Characterizing the natural structural repertoire of LPS is challenging due to the high heterogeneity, branched architecture, and strong amphipathic character of these glycolipids.
34807566	2	49	theme	structural	415:424	arg1	repertoire					426:435	the natural structural repertoire	403:435	the natural structural repertoire of LPS	403:442	Characterizing the natural structural repertoire of LPS is challenging due to the high heterogeneity, branched architecture, and strong amphipathic character of these glycolipids.
34807566	5	50	theme	pathogenic	1081:1090	arg1	serotype					1137:1144	Salmonella enterica serotype Typhimurium	1117:1156	Salmonella enterica serotype Typhimurium	1117:1156	Furthermore, the method enabled the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4.
34807566	5	50	theme	pathogenic	1081:1090	arg1	bacteria					1100:1107	pathogenic enteric bacteria	1081:1107	pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium	1081:1156	Furthermore, the method enabled the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4.
34807566	5	51	dep	Escherichia	1162:1172	arg1	coli					1174:1177	coli	1174:1177	coli	1174:1177	Furthermore, the method enabled the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4.
34807566	9	52	theme	bacterial	1775:1783	arg1	mixtures					1789:1796	complex bacterial LPS mixtures	1767:1796	complex bacterial LPS mixtures	1767:1796	Our results demonstrate that nLC-ESI-FT-MS is an attractive strategy for the structural profiling of small quantities of complex bacterial LPS mixtures in their intact form.
34807566	7	53	theme	lipid	1513:1517	arg1	oligo/polysaccharides					1487:1507	oligo/polysaccharides	1487:1507	oligo/polysaccharides	1487:1507	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	7	53	theme	lipid	1513:1517	arg1	species					1550:1556	single S-type LPS species	1532:1556	single S-type LPS species	1532:1556	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	7	53	theme	lipid	1513:1517	arg1	domains					1521:1527	lipid A domains	1513:1527	lipid A domains	1513:1527	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	9	54	theme	mixtures	1789:1796	arg1	quantities					1753:1762	small quantities	1747:1762	small quantities of complex bacterial LPS mixtures	1747:1796	Our results demonstrate that nLC-ESI-FT-MS is an attractive strategy for the structural profiling of small quantities of complex bacterial LPS mixtures in their intact form.
34807566	7	55	theme	S-type	1539:1544	arg1	species					1550:1556	single S-type LPS species	1532:1556	single S-type LPS species	1532:1556	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	3	56	theme	electrospray	789:800	arg1	ESI-FT-MS					850:858	ESI-FT-MS	850:858	ESI-FT-MS	850:858	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	3	56	theme	electrospray	789:800	arg1	spectrometry					836:847	electrospray ionization Fourier transform mass spectrometry	789:847	electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS)	789:859	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	1	57	theme	Gram-negative	200:212	arg1	bacteria					214:221	Gram-negative bacteria	200:221	Gram-negative bacteria	200:221	The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria contributes to bacterial pathogenesis through various mechanisms, including the development of antibiotic resistance and evasion of the immune response of the host.
34807566	7	58	theme	LPS	1546:1548	arg1	species					1550:1556	single S-type LPS species	1532:1556	single S-type LPS species	1532:1556	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	4	59	theme	Neisseria	914:922	arg1	meningitidis					924:935	Neisseria meningitidis	914:935	Neisseria meningitidis	914:935	Nanogram quantities of rough-type LPS mixtures from Neisseria meningitidis could be separated and analyzed by nLC-ESI-FT-MS.
34807566	3	60	dep	separation	633:642	arg1	the					629:631	the	629:631	the	629:631	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	7	61	theme	A	1519:1519	arg1	oligo/polysaccharides					1487:1507	oligo/polysaccharides	1487:1507	oligo/polysaccharides	1487:1507	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	7	61	theme	A	1519:1519	arg1	species					1550:1556	single S-type LPS species	1532:1556	single S-type LPS species	1532:1556	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	7	61	theme	A	1519:1519	arg1	domains					1521:1527	lipid A domains	1513:1527	lipid A domains	1513:1527	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	3	62	theme	mass	831:834	arg1	ESI-FT-MS					850:858	ESI-FT-MS	850:858	ESI-FT-MS	850:858	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	3	62	theme	mass	831:834	arg1	spectrometry					836:847	electrospray ionization Fourier transform mass spectrometry	789:847	electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS)	789:859	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	6	63	theme	intact	1239:1244	arg1	LPS					1246:1248	intact LPS	1239:1248	intact LPS containing various lengths of the O-specific polysaccharide in the range of 3 and 15 kDa	1239:1337	High-resolution, accurate mass spectra of intact LPS containing various lengths of the O-specific polysaccharide in the range of 3 and 15 kDa were obtained.
34807566	3	64	theme	Fourier	813:819	arg1	ESI-FT-MS					850:858	ESI-FT-MS	850:858	ESI-FT-MS	850:858	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	3	64	theme	Fourier	813:819	arg1	spectrometry					836:847	electrospray ionization Fourier transform mass spectrometry	789:847	electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS)	789:859	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	7	65	with	experiments	1373:1383	arg1	dissociation					1408:1419	collision-induced dissociation	1390:1419	collision-induced dissociation of intact LPS	1390:1433	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	5	66	theme	Escherichia	1162:1172	arg1	O111					1188:1191	Escherichia coli serotype O111	1162:1191	Escherichia coli serotype O111	1162:1191	Furthermore, the method enabled the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4.
34807566	5	67	from	bacteria	1100:1107	arg1	analysis					1023:1030	the analysis	1019:1030	the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4	1019:1194	Furthermore, the method enabled the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4.
34807566	5	67	from	bacteria	1100:1107	arg1	LPS					1072:1074	highly heterogeneous smooth (S)-type LPS	1035:1074	highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium	1035:1156	Furthermore, the method enabled the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4.
34807566	5	67	from	bacteria	1100:1107	arg1	O111					1188:1191	Escherichia coli serotype O111	1162:1191	Escherichia coli serotype O111	1162:1191	Furthermore, the method enabled the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4.
34807566	1	68	theme	bacterial	238:246	arg1	pathogenesis					248:259	bacterial pathogenesis	238:259	bacterial pathogenesis	238:259	The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria contributes to bacterial pathogenesis through various mechanisms, including the development of antibiotic resistance and evasion of the immune response of the host.
34807566	5	69	theme	serotype	1179:1186	arg1	O111					1188:1191	Escherichia coli serotype O111	1162:1191	Escherichia coli serotype O111	1162:1191	Furthermore, the method enabled the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4.
34807566	5	70	theme	enterica	1128:1135	arg1	serotype					1137:1144	Salmonella enterica serotype Typhimurium	1117:1156	Salmonella enterica serotype Typhimurium	1117:1156	Furthermore, the method enabled the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4.
34807566	8	71	theme	S-type	1591:1596	arg1	LPS					1598:1600	S-type LPS	1591:1600	S-type LPS	1591:1600	The structural heterogeneity of S-type LPS was characterized by unprecedented details.
34807566	6	72	theme	polysaccharide	1295:1308	arg1	lengths					1269:1275	various lengths	1261:1275	various lengths of the O-specific polysaccharide	1261:1308	High-resolution, accurate mass spectra of intact LPS containing various lengths of the O-specific polysaccharide in the range of 3 and 15 kDa were obtained.
34807566	3	73	theme	structural	648:657	arg1	profiling					659:667	structural profiling	648:667	structural profiling	648:667	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	4	74	from	quantities	871:880	arg1	meningitidis					924:935	Neisseria meningitidis	914:935	Neisseria meningitidis	914:935	Nanogram quantities of rough-type LPS mixtures from Neisseria meningitidis could be separated and analyzed by nLC-ESI-FT-MS.
34807566	1	75	theme	membrane	163:170	arg1	modification					137:148	The variable modification	124:148	The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria	124:221	The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria contributes to bacterial pathogenesis through various mechanisms, including the development of antibiotic resistance and evasion of the immune response of the host.
34807566	5	76	dep	heterogeneous	1042:1054	arg1	-type					1066:1070	-type	1066:1070	-type	1066:1070	Furthermore, the method enabled the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4.
34807566	0	77	theme	Bacterial	93:101	arg1	Lipopolysaccharides					103:121	Complex Bacterial Lipopolysaccharides	85:121	Complex Bacterial Lipopolysaccharides	85:121	Nanoflow LC-MS Method Allowing In-Depth Characterization of Natural Heterogeneity of Complex Bacterial Lipopolysaccharides.
34807566	5	78	theme	O111	1188:1191	arg1	analysis					1023:1030	the analysis	1019:1030	the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4	1019:1194	Furthermore, the method enabled the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4.
34807566	2	79	theme	amphipathic	524:534	arg1	character					536:544	strong amphipathic character	517:544	strong amphipathic character	517:544	Characterizing the natural structural repertoire of LPS is challenging due to the high heterogeneity, branched architecture, and strong amphipathic character of these glycolipids.
34807566	9	80	theme	small	1747:1751	arg1	quantities					1753:1762	small quantities	1747:1762	small quantities of complex bacterial LPS mixtures	1747:1796	Our results demonstrate that nLC-ESI-FT-MS is an attractive strategy for the structural profiling of small quantities of complex bacterial LPS mixtures in their intact form.
34807566	0	81	gly	Heterogeneity	68:80	arg1	Lipopolysaccharides					103:121	Complex Bacterial Lipopolysaccharides	85:121	Complex Bacterial Lipopolysaccharides	85:121	Nanoflow LC-MS Method Allowing In-Depth Characterization of Natural Heterogeneity of Complex Bacterial Lipopolysaccharides.
34807566	2	82	dep	heterogeneity	475:487	arg1	the					466:468	the	466:468	the	466:468	Characterizing the natural structural repertoire of LPS is challenging due to the high heterogeneity, branched architecture, and strong amphipathic character of these glycolipids.
34807566	2	83	theme	glycolipids	555:565	arg1	heterogeneity					475:487	high heterogeneity	470:487	high heterogeneity	470:487	Characterizing the natural structural repertoire of LPS is challenging due to the high heterogeneity, branched architecture, and strong amphipathic character of these glycolipids.
34807566	2	83	theme	glycolipids	555:565	arg1	architecture					499:510	branched architecture	490:510	branched architecture	490:510	Characterizing the natural structural repertoire of LPS is challenging due to the high heterogeneity, branched architecture, and strong amphipathic character of these glycolipids.
34807566	2	83	theme	glycolipids	555:565	arg1	character					536:544	strong amphipathic character	517:544	strong amphipathic character	517:544	Characterizing the natural structural repertoire of LPS is challenging due to the high heterogeneity, branched architecture, and strong amphipathic character of these glycolipids.
34807566	2	84	theme	LPS	440:442	arg1	LPS					440:442	LPS	440:442	LPS	440:442	Characterizing the natural structural repertoire of LPS is challenging due to the high heterogeneity, branched architecture, and strong amphipathic character of these glycolipids.
34807566	2	84	theme	LPS	440:442	arg1	repertoire					426:435	the natural structural repertoire	403:435	the natural structural repertoire of LPS	403:442	Characterizing the natural structural repertoire of LPS is challenging due to the high heterogeneity, branched architecture, and strong amphipathic character of these glycolipids.
34807566	0	85	theme	LC-MS	9:13	arg1	Method					15:20	Nanoflow LC-MS Method	0:20	Nanoflow LC-MS Method	0:20	Nanoflow LC-MS Method Allowing In-Depth Characterization of Natural Heterogeneity of Complex Bacterial Lipopolysaccharides.
34807566	7	86	from	information	1453:1463	arg1	oligo/polysaccharides					1487:1507	oligo/polysaccharides	1487:1507	oligo/polysaccharides	1487:1507	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	7	86	from	information	1453:1463	arg1	composition					1472:1482	the composition	1468:1482	the composition of oligo/polysaccharides	1468:1507	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	7	86	from	information	1453:1463	arg1	species					1550:1556	single S-type LPS species	1532:1556	single S-type LPS species	1532:1556	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	7	86	from	information	1453:1463	arg1	domains					1521:1527	lipid A domains	1513:1527	lipid A domains	1513:1527	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	7	87	theme	collision-induced	1390:1406	arg1	dissociation					1408:1419	collision-induced dissociation	1390:1419	collision-induced dissociation of intact LPS	1390:1433	In addition, MS/MS experiments with collision-induced dissociation of intact LPS provided detailed information on the composition of oligo/polysaccharides and lipid A domains of single S-type LPS species.
34807566	1	88	theme	resistance	329:338	arg1	evasion					344:350	evasion	344:350	evasion of the immune response of the host	344:385	The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria contributes to bacterial pathogenesis through various mechanisms, including the development of antibiotic resistance and evasion of the immune response of the host.
34807566	1	88	theme	resistance	329:338	arg1	development					303:313	the development	299:313	the development of antibiotic resistance	299:338	The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria contributes to bacterial pathogenesis through various mechanisms, including the development of antibiotic resistance and evasion of the immune response of the host.
34807566	9	89	theme	attractive	1695:1704	arg1	nLC-ESI-FT-MS					1675:1687	nLC-ESI-FT-MS	1675:1687	nLC-ESI-FT-MS	1675:1687	Our results demonstrate that nLC-ESI-FT-MS is an attractive strategy for the structural profiling of small quantities of complex bacterial LPS mixtures in their intact form.
34807566	9	89	theme	attractive	1695:1704	arg1	strategy					1706:1713	an attractive strategy	1692:1713	an attractive strategy for the structural profiling of small quantities of complex bacterial LPS mixtures in their intact form	1692:1817	Our results demonstrate that nLC-ESI-FT-MS is an attractive strategy for the structural profiling of small quantities of complex bacterial LPS mixtures in their intact form.
34807566	9	90	from	profiling	1734:1742	arg1	form					1814:1817	their intact form	1801:1817	their intact form	1801:1817	Our results demonstrate that nLC-ESI-FT-MS is an attractive strategy for the structural profiling of small quantities of complex bacterial LPS mixtures in their intact form.
34807566	2	91	theme	high	470:473	arg1	heterogeneity					475:487	high heterogeneity	470:487	high heterogeneity	470:487	Characterizing the natural structural repertoire of LPS is challenging due to the high heterogeneity, branched architecture, and strong amphipathic character of these glycolipids.
34807566	3	92	theme	mixtures	691:698	arg1	separation					633:642	separation	633:642	separation	633:642	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	3	92	theme	mixtures	691:698	arg1	profiling					659:667	structural profiling	648:667	structural profiling	648:667	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	5	93	theme	enteric	1092:1098	arg1	serotype					1137:1144	Salmonella enterica serotype Typhimurium	1117:1156	Salmonella enterica serotype Typhimurium	1117:1156	Furthermore, the method enabled the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4.
34807566	5	93	theme	enteric	1092:1098	arg1	bacteria					1100:1107	pathogenic enteric bacteria	1081:1107	pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium	1081:1156	Furthermore, the method enabled the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4.
34807566	3	94	theme	intact	680:685	arg1	mixtures					691:698	complex intact LPS mixtures	672:698	complex intact LPS mixtures	672:698	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	4	95	theme	rough-type	885:894	arg1	mixtures					900:907	rough-type LPS mixtures	885:907	rough-type LPS mixtures from Neisseria meningitidis	885:935	Nanogram quantities of rough-type LPS mixtures from Neisseria meningitidis could be separated and analyzed by nLC-ESI-FT-MS.
34807566	0	96	theme	Natural	60:66	arg1	Heterogeneity					68:80	Natural Heterogeneity	60:80	Natural Heterogeneity of Complex Bacterial Lipopolysaccharides	60:121	Nanoflow LC-MS Method Allowing In-Depth Characterization of Natural Heterogeneity of Complex Bacterial Lipopolysaccharides.
34807566	9	97	theme	structural	1723:1732	arg1	profiling					1734:1742	the structural profiling	1719:1742	the structural profiling of small quantities of complex bacterial LPS mixtures in their intact form	1719:1817	Our results demonstrate that nLC-ESI-FT-MS is an attractive strategy for the structural profiling of small quantities of complex bacterial LPS mixtures in their intact form.
34807566	6	98	theme	mass	1223:1226	arg1	spectra					1228:1234	High-resolution, accurate mass spectra	1197:1234	High-resolution, accurate mass spectra of intact LPS containing various lengths of the O-specific polysaccharide in the range of 3 and 15 kDa	1197:1337	High-resolution, accurate mass spectra of intact LPS containing various lengths of the O-specific polysaccharide in the range of 3 and 15 kDa were obtained.
34807566	3	99	theme	reversed-phase	718:731	arg1	nLC					773:775	nLC	773:775	nLC	773:775	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	3	99	theme	reversed-phase	718:731	arg1	chromatography					757:770	nanoflow reversed-phase high-performance liquid chromatography	709:770	nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS)	709:859	To address this problem, we have developed a method enabling the separation and structural profiling of complex intact LPS mixtures by using nanoflow reversed-phase high-performance liquid chromatography (nLC) coupled to electrospray ionization Fourier transform mass spectrometry (ESI-FT-MS).
34807566	4	100	theme	mixtures	900:907	arg1	quantities					871:880	Nanogram quantities	862:880	Nanogram quantities of rough-type LPS mixtures from Neisseria meningitidis	862:935	Nanogram quantities of rough-type LPS mixtures from Neisseria meningitidis could be separated and analyzed by nLC-ESI-FT-MS.
34807566	1	101	theme	response	366:373	arg1	evasion					344:350	evasion	344:350	evasion of the immune response of the host	344:385	The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria contributes to bacterial pathogenesis through various mechanisms, including the development of antibiotic resistance and evasion of the immune response of the host.
34807566	1	101	theme	response	366:373	arg1	development					303:313	the development	299:313	the development of antibiotic resistance	299:338	The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria contributes to bacterial pathogenesis through various mechanisms, including the development of antibiotic resistance and evasion of the immune response of the host.
34807566	6	102	contain	containing	1250:1259	arg2	lengths					1269:1275	various lengths	1261:1275	various lengths of the O-specific polysaccharide	1261:1308	High-resolution, accurate mass spectra of intact LPS containing various lengths of the O-specific polysaccharide in the range of 3 and 15 kDa were obtained.
34807566	6	102	contain	containing	1250:1259	arg1	LPS					1246:1248	intact LPS	1239:1248	intact LPS containing various lengths of the O-specific polysaccharide in the range of 3 and 15 kDa	1239:1337	High-resolution, accurate mass spectra of intact LPS containing various lengths of the O-specific polysaccharide in the range of 3 and 15 kDa were obtained.
34807566	6	103	dep	High-resolution	1197:1211	arg1	accurate					1214:1221	accurate	1214:1221	accurate	1214:1221	High-resolution, accurate mass spectra of intact LPS containing various lengths of the O-specific polysaccharide in the range of 3 and 15 kDa were obtained.
34807566	5	104	from	analysis	1023:1030	arg1	serotype					1137:1144	Salmonella enterica serotype Typhimurium	1117:1156	Salmonella enterica serotype Typhimurium	1117:1156	Furthermore, the method enabled the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4.
34807566	5	104	from	analysis	1023:1030	arg1	bacteria					1100:1107	pathogenic enteric bacteria	1081:1107	pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium	1081:1156	Furthermore, the method enabled the analysis of highly heterogeneous smooth (S)-type LPS from pathogenic enteric bacteria such as Salmonella enterica serotype Typhimurium and Escherichia coli serotype O111:B4.
34807566	1	105	theme	various	269:275	arg1	mechanisms					277:286	various mechanisms	269:286	various mechanisms	269:286	The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria contributes to bacterial pathogenesis through various mechanisms, including the development of antibiotic resistance and evasion of the immune response of the host.
34807566	1	105	theme	various	269:275	arg1	evasion					344:350	evasion	344:350	evasion of the immune response of the host	344:385	The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria contributes to bacterial pathogenesis through various mechanisms, including the development of antibiotic resistance and evasion of the immune response of the host.
34807566	1	105	theme	various	269:275	arg1	development					303:313	the development	299:313	the development of antibiotic resistance	299:338	The variable modification of the outer membrane lipopolysaccharide (LPS) in Gram-negative bacteria contributes to bacterial pathogenesis through various mechanisms, including the development of antibiotic resistance and evasion of the immune response of the host.
34807566	9	106	theme	quantities	1753:1762	arg1	profiling					1734:1742	the structural profiling	1719:1742	the structural profiling of small quantities of complex bacterial LPS mixtures in their intact form	1719:1817	Our results demonstrate that nLC-ESI-FT-MS is an attractive strategy for the structural profiling of small quantities of complex bacterial LPS mixtures in their intact form.
34807566	2	107	theme	natural	407:413	arg1	LPS					440:442	LPS	440:442	LPS	440:442	Characterizing the natural structural repertoire of LPS is challenging due to the high heterogeneity, branched architecture, and strong amphipathic character of these glycolipids.
34807566	2	107	theme	natural	407:413	arg1	repertoire					426:435	the natural structural repertoire	403:435	the natural structural repertoire of LPS	403:442	Characterizing the natural structural repertoire of LPS is challenging due to the high heterogeneity, branched architecture, and strong amphipathic character of these glycolipids.
33277973	3	0	theme	%	539:539	arg1	nanoplatelets					560:572	0.5% (wt/vol) Laponite® nanoplatelets	536:572	0.5% (wt/vol) Laponite® nanoplatelets	536:572	The results showed that the addition of 0.5% (wt/vol) Laponite® nanoplatelets improved the printability of ADA-GEL hydrogels enabling the fabrication of detailed structures since a low effect of material spreading and reduced tendency to pore closure appeared.
33277973	5	1	theme	processing	1075:1084	arg1	window					1086:1091	a processing window	1073:1091	a processing window of 1-2 h	1073:1100	A glass flip test determined a processing window of 1-2 h after composite ink preparation.
33277973	4	2	dep	types	803:807	arg1	conical					826:832	conical	826:832	conical	826:832	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33277973	4	2	dep	types	803:807	arg1	cylindrical					810:820	cylindrical	810:820	cylindrical	810:820	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33277973	1	3	theme	successful	278:287	arg1	biofabrication					292:305	successful 3D biofabrication	278:305	successful 3D biofabrication	278:305	The concept of adding inorganic fillers into hydrogels to form hydrogel nanocomposites often provides advantageous properties which can be exploited for successful 3D biofabrication.
33277973	0	4	theme	3D	112:113	arg1	printing					115:122	extrusion-based 3D printing	96:122	extrusion-based 3D printing	96:122	Potential of Laponite® incorporated oxidized alginate-gelatin (ADA-GEL) composite hydrogels for extrusion-based 3D printing.
33277973	3	5	theme	pore	734:737	arg1	closure					739:745	pore closure	734:745	pore closure	734:745	The results showed that the addition of 0.5% (wt/vol) Laponite® nanoplatelets improved the printability of ADA-GEL hydrogels enabling the fabrication of detailed structures since a low effect of material spreading and reduced tendency to pore closure appeared.
33277973	6	6	theme	promising	1200:1208	arg1	inks					1210:1213	promising inks	1200:1213	promising inks for 3D bioprinting	1200:1232	Overall, Laponite® /ADA-GEL hydrogel composites are confirmed as promising inks for 3D bioprinting.
33277973	6	6	theme	promising	1200:1208	arg1	composites					1172:1181	Laponite® /ADA-GEL hydrogel composites	1144:1181	Laponite® /ADA-GEL hydrogel composites	1144:1181	Overall, Laponite® /ADA-GEL hydrogel composites are confirmed as promising inks for 3D bioprinting.
33277973	6	7	theme	3D	1219:1220	arg1	bioprinting					1222:1232	3D bioprinting	1219:1232	3D bioprinting	1219:1232	Overall, Laponite® /ADA-GEL hydrogel composites are confirmed as promising inks for 3D bioprinting.
33277973	1	8	theme	3D	289:290	arg1	biofabrication					292:305	successful 3D biofabrication	278:305	successful 3D biofabrication	278:305	The concept of adding inorganic fillers into hydrogels to form hydrogel nanocomposites often provides advantageous properties which can be exploited for successful 3D biofabrication.
33277973	0	9	theme	extrusion-based	96:110	arg1	printing					115:122	extrusion-based 3D printing	96:122	extrusion-based 3D printing	96:122	Potential of Laponite® incorporated oxidized alginate-gelatin (ADA-GEL) composite hydrogels for extrusion-based 3D printing.
33277973	5	10	theme	ink	1118:1120	arg1	preparation					1122:1132	composite ink preparation	1108:1132	composite ink preparation	1108:1132	A glass flip test determined a processing window of 1-2 h after composite ink preparation.
33277973	4	11	theme	types	803:807	arg1	comparison					772:781	a comparison	770:781	a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test	770:888	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33277973	6	12	theme	hydrogel	1163:1170	arg1	inks					1210:1213	promising inks	1200:1213	promising inks for 3D bioprinting	1200:1232	Overall, Laponite® /ADA-GEL hydrogel composites are confirmed as promising inks for 3D bioprinting.
33277973	6	12	theme	hydrogel	1163:1170	arg1	composites					1172:1181	Laponite® /ADA-GEL hydrogel composites	1144:1181	Laponite® /ADA-GEL hydrogel composites	1144:1181	Overall, Laponite® /ADA-GEL hydrogel composites are confirmed as promising inks for 3D bioprinting.
33277973	4	13	theme	filament	869:876	arg1	test					885:888	filament fusion test	869:888	filament fusion test	869:888	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33277973	3	14	theme	Laponite®	550:558	arg1	nanoplatelets					560:572	0.5% (wt/vol) Laponite® nanoplatelets	536:572	0.5% (wt/vol) Laponite® nanoplatelets	536:572	The results showed that the addition of 0.5% (wt/vol) Laponite® nanoplatelets improved the printability of ADA-GEL hydrogels enabling the fabrication of detailed structures since a low effect of material spreading and reduced tendency to pore closure appeared.
33277973	1	15	theme	hydrogel	188:195	arg1	nanocomposites					197:210	hydrogel nanocomposites	188:210	hydrogel nanocomposites	188:210	The concept of adding inorganic fillers into hydrogels to form hydrogel nanocomposites often provides advantageous properties which can be exploited for successful 3D biofabrication.
33277973	4	16	theme	conical	1031:1037	arg1	tip					1039:1041	conical tip	1031:1041	conical tip	1031:1041	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33277973	3	17	theme	nanoplatelets	560:572	arg1	addition					524:531	the addition	520:531	the addition of 0.5% (wt/vol) Laponite® nanoplatelets	520:572	The results showed that the addition of 0.5% (wt/vol) Laponite® nanoplatelets improved the printability of ADA-GEL hydrogels enabling the fabrication of detailed structures since a low effect of material spreading and reduced tendency to pore closure appeared.
33277973	2	18	theme	composite	329:337	arg1	hydrogel					339:346	a new composite hydrogel	323:346	a new composite hydrogel combining oxidized alginate-gelatin (ADA-GEL) hydrogel and Laponite® nanoclay as inorganic nanofiller	323:448	In this study, a new composite hydrogel combining oxidized alginate-gelatin (ADA-GEL) hydrogel and Laponite® nanoclay as inorganic nanofiller was successfully developed and characterized.
33277973	2	19	theme	Laponite®	407:415	arg1	nanoclay					417:424	Laponite® nanoclay	407:424	Laponite® nanoclay	407:424	In this study, a new composite hydrogel combining oxidized alginate-gelatin (ADA-GEL) hydrogel and Laponite® nanoclay as inorganic nanofiller was successfully developed and characterized.
33277973	4	20	theme	fusion	878:883	arg1	test					885:888	filament fusion test	869:888	filament fusion test	869:888	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33277973	2	21	theme	new	325:327	arg1	hydrogel					339:346	a new composite hydrogel	323:346	a new composite hydrogel combining oxidized alginate-gelatin (ADA-GEL) hydrogel and Laponite® nanoclay as inorganic nanofiller	323:448	In this study, a new composite hydrogel combining oxidized alginate-gelatin (ADA-GEL) hydrogel and Laponite® nanoclay as inorganic nanofiller was successfully developed and characterized.
33277973	5	22	theme	1-2 h	1096:1100	arg1	window					1086:1091	a processing window	1073:1091	a processing window of 1-2 h	1073:1100	A glass flip test determined a processing window of 1-2 h after composite ink preparation.
33277973	4	23	theme	inner	840:844	arg1	diameter					846:853	same inner diameter	835:853	cylindrical and conical; same inner diameter of 250 μm	810:863	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33277973	4	24	theme	enhanced	947:954	arg1	accuracy					965:972	enhanced printing accuracy	947:972	enhanced printing accuracy	947:972	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33277973	4	25	from	comparison	772:781	arg1	test					885:888	filament fusion test	869:888	filament fusion test	869:888	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33277973	4	26	theme	needle	796:801	arg1	types					803:807	different needle types	786:807	different needle types (cylindrical and conical; same inner diameter of 250 μm)	786:864	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33277973	0	27	theme	Laponite®	13:21	arg1	Potential					0:8	Potential	0:8	Potential of Laponite®	0:21	Potential of Laponite® incorporated oxidized alginate-gelatin (ADA-GEL) composite hydrogels for extrusion-based 3D printing.
33277973	4	28	theme	different	786:794	arg1	types					803:807	different needle types	786:807	different needle types (cylindrical and conical; same inner diameter of 250 μm)	786:864	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33277973	4	29	contain	has	943:945	arg2	accuracy					965:972	enhanced printing accuracy	947:972	enhanced printing accuracy	947:972	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33277973	4	29	contain	has	943:945	arg2	fidelity					986:993	pattern fidelity	978:993	pattern fidelity	978:993	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33277973	4	29	contain	has	943:945	arg1	pattern					906:912	the pattern	902:912	the pattern dispensed by cylindrical tip	902:941	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33277973	0	30	theme	alginate-gelatin	45:60	arg1	hydrogels					82:90	oxidized alginate-gelatin (ADA-GEL) composite hydrogels	36:90	oxidized alginate-gelatin (ADA-GEL) composite hydrogels for extrusion-based 3D printing	36:122	Potential of Laponite® incorporated oxidized alginate-gelatin (ADA-GEL) composite hydrogels for extrusion-based 3D printing.
33277973	6	31	theme	/ADA-GEL	1154:1161	arg1	inks					1210:1213	promising inks	1200:1213	promising inks for 3D bioprinting	1200:1232	Overall, Laponite® /ADA-GEL hydrogel composites are confirmed as promising inks for 3D bioprinting.
33277973	6	31	theme	/ADA-GEL	1154:1161	arg1	composites					1172:1181	Laponite® /ADA-GEL hydrogel composites	1144:1181	Laponite® /ADA-GEL hydrogel composites	1144:1181	Overall, Laponite® /ADA-GEL hydrogel composites are confirmed as promising inks for 3D bioprinting.
33277973	3	32	theme	material	691:698	arg1	spreading					700:708	material spreading	691:708	material spreading	691:708	The results showed that the addition of 0.5% (wt/vol) Laponite® nanoplatelets improved the printability of ADA-GEL hydrogels enabling the fabrication of detailed structures since a low effect of material spreading and reduced tendency to pore closure appeared.
33277973	4	33	theme	same	835:838	arg1	diameter					846:853	same inner diameter	835:853	cylindrical and conical; same inner diameter of 250 μm	810:863	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33277973	1	34	theme	advantageous	227:238	arg1	properties					240:249	advantageous properties	227:249	advantageous properties which can be exploited for successful 3D biofabrication	227:305	The concept of adding inorganic fillers into hydrogels to form hydrogel nanocomposites often provides advantageous properties which can be exploited for successful 3D biofabrication.
33277973	0	35	theme	oxidized	36:43	arg1	hydrogels					82:90	oxidized alginate-gelatin (ADA-GEL) composite hydrogels	36:90	oxidized alginate-gelatin (ADA-GEL) composite hydrogels for extrusion-based 3D printing	36:122	Potential of Laponite® incorporated oxidized alginate-gelatin (ADA-GEL) composite hydrogels for extrusion-based 3D printing.
33277973	4	36	theme	printing	956:963	arg1	accuracy					965:972	enhanced printing accuracy	947:972	enhanced printing accuracy	947:972	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33277973	3	37	theme	low	677:679	arg1	effect					681:686	a low effect	675:686	a low effect of material spreading and reduced tendency to pore closure	675:745	The results showed that the addition of 0.5% (wt/vol) Laponite® nanoplatelets improved the printability of ADA-GEL hydrogels enabling the fabrication of detailed structures since a low effect of material spreading and reduced tendency to pore closure appeared.
33277973	5	38	theme	glass	1046:1050	arg1	test					1057:1060	A glass flip test	1044:1060	A glass flip test	1044:1060	A glass flip test determined a processing window of 1-2 h after composite ink preparation.
33277973	2	39	theme	inorganic	429:437	arg1	nanofiller					439:448	inorganic nanofiller	429:448	inorganic nanofiller	429:448	In this study, a new composite hydrogel combining oxidized alginate-gelatin (ADA-GEL) hydrogel and Laponite® nanoclay as inorganic nanofiller was successfully developed and characterized.
33277973	0	40	theme	ADA-GEL	63:69	arg1	hydrogels					82:90	oxidized alginate-gelatin (ADA-GEL) composite hydrogels	36:90	oxidized alginate-gelatin (ADA-GEL) composite hydrogels for extrusion-based 3D printing	36:122	Potential of Laponite® incorporated oxidized alginate-gelatin (ADA-GEL) composite hydrogels for extrusion-based 3D printing.
33277973	4	41	theme	pattern	978:984	arg1	fidelity					986:993	pattern fidelity	978:993	pattern fidelity	978:993	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33277973	5	42	theme	composite	1108:1116	arg1	preparation					1122:1132	composite ink preparation	1108:1132	composite ink preparation	1108:1132	A glass flip test determined a processing window of 1-2 h after composite ink preparation.
33277973	5	43	theme	flip	1052:1055	arg1	test					1057:1060	A glass flip test	1044:1060	A glass flip test	1044:1060	A glass flip test determined a processing window of 1-2 h after composite ink preparation.
33277973	2	44	theme	alginate-gelatin	367:382	arg1	hydrogel					394:401	oxidized alginate-gelatin (ADA-GEL) hydrogel	358:401	oxidized alginate-gelatin (ADA-GEL) hydrogel	358:401	In this study, a new composite hydrogel combining oxidized alginate-gelatin (ADA-GEL) hydrogel and Laponite® nanoclay as inorganic nanofiller was successfully developed and characterized.
33277973	3	45	theme	reduced	714:720	arg1	tendency					722:729	reduced tendency	714:729	reduced tendency to pore closure	714:745	The results showed that the addition of 0.5% (wt/vol) Laponite® nanoplatelets improved the printability of ADA-GEL hydrogels enabling the fabrication of detailed structures since a low effect of material spreading and reduced tendency to pore closure appeared.
33277973	4	46	theme	250 μm	858:863	arg1	diameter					846:853	same inner diameter	835:853	cylindrical and conical; same inner diameter of 250 μm	810:863	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33277973	2	47	theme	oxidized	358:365	arg1	hydrogel					394:401	oxidized alginate-gelatin (ADA-GEL) hydrogel	358:401	oxidized alginate-gelatin (ADA-GEL) hydrogel	358:401	In this study, a new composite hydrogel combining oxidized alginate-gelatin (ADA-GEL) hydrogel and Laponite® nanoclay as inorganic nanofiller was successfully developed and characterized.
33277973	3	48	theme	ADA-GEL	603:609	arg1	hydrogels					611:619	ADA-GEL hydrogels	603:619	ADA-GEL hydrogels enabling the fabrication of detailed structures	603:667	The results showed that the addition of 0.5% (wt/vol) Laponite® nanoplatelets improved the printability of ADA-GEL hydrogels enabling the fabrication of detailed structures since a low effect of material spreading and reduced tendency to pore closure appeared.
33277973	3	49	theme	tendency	722:729	arg1	effect					681:686	a low effect	675:686	a low effect of material spreading and reduced tendency to pore closure	675:745	The results showed that the addition of 0.5% (wt/vol) Laponite® nanoplatelets improved the printability of ADA-GEL hydrogels enabling the fabrication of detailed structures since a low effect of material spreading and reduced tendency to pore closure appeared.
33277973	6	50	theme	Laponite®	1144:1152	arg1	inks					1210:1213	promising inks	1200:1213	promising inks for 3D bioprinting	1200:1232	Overall, Laponite® /ADA-GEL hydrogel composites are confirmed as promising inks for 3D bioprinting.
33277973	6	50	theme	Laponite®	1144:1152	arg1	composites					1172:1181	Laponite® /ADA-GEL hydrogel composites	1144:1181	Laponite® /ADA-GEL hydrogel composites	1144:1181	Overall, Laponite® /ADA-GEL hydrogel composites are confirmed as promising inks for 3D bioprinting.
33277973	4	51	theme	cylindrical	927:937	arg1	tip					939:941	cylindrical tip	927:941	cylindrical tip	927:941	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33277973	3	52	theme	spreading	700:708	arg1	effect					681:686	a low effect	675:686	a low effect of material spreading and reduced tendency to pore closure	675:745	The results showed that the addition of 0.5% (wt/vol) Laponite® nanoplatelets improved the printability of ADA-GEL hydrogels enabling the fabrication of detailed structures since a low effect of material spreading and reduced tendency to pore closure appeared.
33277973	4	53	dep	cylindrical	810:820	arg1	diameter					846:853	same inner diameter	835:853	cylindrical and conical; same inner diameter of 250 μm	810:863	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33277973	0	54	theme	composite	72:80	arg1	hydrogels					82:90	oxidized alginate-gelatin (ADA-GEL) composite hydrogels	36:90	oxidized alginate-gelatin (ADA-GEL) composite hydrogels for extrusion-based 3D printing	36:122	Potential of Laponite® incorporated oxidized alginate-gelatin (ADA-GEL) composite hydrogels for extrusion-based 3D printing.
33277973	3	55	theme	detailed	649:656	arg1	structures					658:667	detailed structures	649:667	detailed structures	649:667	The results showed that the addition of 0.5% (wt/vol) Laponite® nanoplatelets improved the printability of ADA-GEL hydrogels enabling the fabrication of detailed structures since a low effect of material spreading and reduced tendency to pore closure appeared.
33277973	1	56	theme	inorganic	147:155	arg1	fillers					157:163	inorganic fillers	147:163	inorganic fillers into hydrogels	147:178	The concept of adding inorganic fillers into hydrogels to form hydrogel nanocomposites often provides advantageous properties which can be exploited for successful 3D biofabrication.
33277973	3	57	theme	structures	658:667	arg1	fabrication					634:644	the fabrication	630:644	the fabrication of detailed structures	630:667	The results showed that the addition of 0.5% (wt/vol) Laponite® nanoplatelets improved the printability of ADA-GEL hydrogels enabling the fabrication of detailed structures since a low effect of material spreading and reduced tendency to pore closure appeared.
33277973	3	58	theme	hydrogels	611:619	arg1	printability					587:598	the printability	583:598	the printability of ADA-GEL hydrogels enabling the fabrication of detailed structures	583:667	The results showed that the addition of 0.5% (wt/vol) Laponite® nanoplatelets improved the printability of ADA-GEL hydrogels enabling the fabrication of detailed structures since a low effect of material spreading and reduced tendency to pore closure appeared.
33277973	4	59	from	tip	1039:1041	arg1	pattern					1018:1024	the pattern	1014:1024	the pattern from conical tip	1014:1041	Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip.
33090346	6	0	theme	fast	969:972	arg1	efficiency					985:994	good and fast absorption efficiency	960:994	good and fast absorption efficiency	960:994	This kind of CF/C cryogel revealed good and fast absorption efficiency.
33090346	5	1	dep	times	884:888	arg1	weight					894:899	the weight	890:899	up to 6.9-17.7 times the weight of the initial cryogel	869:922	Moreover, its sorption capacity can reach up to 6.9-17.7 times the weight of the initial cryogel.
33090346	7	2	theme	absorption/distillation	1031:1053	arg1	methods					1081:1087	simple absorption/distillation and absorption/desorption methods	1024:1087	simple absorption/distillation and absorption/desorption methods	1024:1087	It could also be reused by simple absorption/distillation and absorption/desorption methods.
33090346	1	3	theme	first	251:255	arg1	time					257:260	the first time	247:260	the first time	247:260	Keratin/cellulose cryogels were successfully fabricated using chicken feathers (CF) and cardboard (C) from environmental waste for the first time, to be exploited in oil/solvent absorption.
33090346	0	4	theme	oils	89:92	arg1	absorption					75:84	absorption	75:84	absorption of oils and organic solvents	75:113	Development of novel and ecological keratin/cellulose-based composites for absorption of oils and organic solvents.
33090346	9	5	theme	spill	1363:1367	arg1	cleanup					1369:1375	spill cleanup	1363:1375	spill cleanup of oils and organic solvents	1363:1404	Besides, owing to its low cost, good absorption capacity, and excellent reusability, this cryogel has potential for spill cleanup of oils and organic solvents.
33090346	8	6	theme	kinetic	1102:1108	arg1	analysis					1110:1117	the kinetic analysis	1098:1117	the kinetic analysis	1098:1117	Through the kinetic analysis, it was found that the pseudo-second-order model was more appropriate for the keratin/cellulose cryogel oil absorption process.
33090346	4	7	theme	as-prepared	761:771	arg1	cryogel					773:779	The as-prepared cryogel	757:779	The as-prepared cryogel	757:779	The as-prepared cryogel can absorb various oils and organic solvents.
33090346	0	8	theme	solvents	106:113	arg1	absorption					75:84	absorption	75:84	absorption of oils and organic solvents	75:113	Development of novel and ecological keratin/cellulose-based composites for absorption of oils and organic solvents.
33090346	6	9	theme	good	960:963	arg1	efficiency					985:994	good and fast absorption efficiency	960:994	good and fast absorption efficiency	960:994	This kind of CF/C cryogel revealed good and fast absorption efficiency.
33090346	3	10	theme	keratin/cellulose-based	587:609	arg1	composites					611:620	the synthesized keratin/cellulose-based composites	571:620	the synthesized keratin/cellulose-based composites	571:620	The characterization analysis of the synthesized keratin/cellulose-based composites was performed using Fourier transform infrared spectrometry, X-ray diffractometry, scanning electron microscopy, and thermogravimetry.
33090346	0	11	theme	organic	98:104	arg1	solvents					106:113	organic solvents	98:113	organic solvents	98:113	Development of novel and ecological keratin/cellulose-based composites for absorption of oils and organic solvents.
33090346	5	12	dep	6.9-17.7	875:882	arg1	to					872:873	to	872:873	to	872:873	Moreover, its sorption capacity can reach up to 6.9-17.7 times the weight of the initial cryogel.
33090346	9	13	theme	low	1269:1271	arg1	cost					1273:1276	its low cost	1265:1276	its low cost	1265:1276	Besides, owing to its low cost, good absorption capacity, and excellent reusability, this cryogel has potential for spill cleanup of oils and organic solvents.
33090346	5	14	theme	cryogel	916:922	arg1	weight					894:899	the weight	890:899	up to 6.9-17.7 times the weight of the initial cryogel	869:922	Moreover, its sorption capacity can reach up to 6.9-17.7 times the weight of the initial cryogel.
33090346	6	15	theme	cryogel	943:949	arg1	kind					930:933	This kind	925:933	This kind of CF/C cryogel	925:949	This kind of CF/C cryogel revealed good and fast absorption efficiency.
33090346	4	16	theme	organic	809:815	arg1	solvents					817:824	organic solvents	809:824	organic solvents	809:824	The as-prepared cryogel can absorb various oils and organic solvents.
33090346	3	17	theme	characterization	542:557	arg1	analysis					559:566	The characterization analysis	538:566	The characterization analysis of the synthesized keratin/cellulose-based composites	538:620	The characterization analysis of the synthesized keratin/cellulose-based composites was performed using Fourier transform infrared spectrometry, X-ray diffractometry, scanning electron microscopy, and thermogravimetry.
33090346	2	18	theme	waste	400:404	arg1	dissolution					376:386	the dissolution	372:386	the dissolution of CF and C waste in 1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent	372:482	The keratin/cellulose-based composites were obtained by combining the dissolution of CF and C waste in 1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent via regeneration, simply by the freeze-drying method.
33090346	2	19	theme	freeze-drying	516:528	arg1	method					530:535	the freeze-drying method	512:535	the freeze-drying method	512:535	The keratin/cellulose-based composites were obtained by combining the dissolution of CF and C waste in 1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent via regeneration, simply by the freeze-drying method.
33090346	6	20	theme	CF/C	938:941	arg1	cryogel					943:949	CF/C cryogel	938:949	CF/C cryogel	938:949	This kind of CF/C cryogel revealed good and fast absorption efficiency.
33090346	2	21	theme	C	398:398	arg1	waste					400:404	CF and C waste	391:404	waste	400:404	The keratin/cellulose-based composites were obtained by combining the dissolution of CF and C waste in 1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent via regeneration, simply by the freeze-drying method.
33090346	2	22	theme	liquid	463:468	arg1	solvent					476:482	1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent	409:482	1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent	409:482	The keratin/cellulose-based composites were obtained by combining the dissolution of CF and C waste in 1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent via regeneration, simply by the freeze-drying method.
33090346	5	23	theme	sorption	841:848	arg1	capacity					850:857	its sorption capacity	837:857	its sorption capacity	837:857	Moreover, its sorption capacity can reach up to 6.9-17.7 times the weight of the initial cryogel.
33090346	9	24	theme	good	1279:1282	arg1	capacity					1295:1302	good absorption capacity	1279:1302	good absorption capacity	1279:1302	Besides, owing to its low cost, good absorption capacity, and excellent reusability, this cryogel has potential for spill cleanup of oils and organic solvents.
33090346	2	25	theme	keratin/cellulose-based	310:332	arg1	composites					334:343	The keratin/cellulose-based composites	306:343	The keratin/cellulose-based composites	306:343	The keratin/cellulose-based composites were obtained by combining the dissolution of CF and C waste in 1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent via regeneration, simply by the freeze-drying method.
33090346	9	26	theme	oils	1380:1383	arg1	cleanup					1369:1375	spill cleanup	1363:1375	spill cleanup of oils and organic solvents	1363:1404	Besides, owing to its low cost, good absorption capacity, and excellent reusability, this cryogel has potential for spill cleanup of oils and organic solvents.
33090346	3	27	dep	Fourier	642:648	arg1	transform					650:658	transform	650:658	transform infrared spectrometry, X-ray diffractometry, scanning electron microscopy, and thermogravimetry	650:754	The characterization analysis of the synthesized keratin/cellulose-based composites was performed using Fourier transform infrared spectrometry, X-ray diffractometry, scanning electron microscopy, and thermogravimetry.
33090346	2	28	theme	ionic	457:461	arg1	solvent					476:482	1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent	409:482	1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent	409:482	The keratin/cellulose-based composites were obtained by combining the dissolution of CF and C waste in 1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent via regeneration, simply by the freeze-drying method.
33090346	9	29	theme	solvents	1397:1404	arg1	cleanup					1369:1375	spill cleanup	1363:1375	spill cleanup of oils and organic solvents	1363:1404	Besides, owing to its low cost, good absorption capacity, and excellent reusability, this cryogel has potential for spill cleanup of oils and organic solvents.
33090346	9	30	theme	absorption	1284:1293	arg1	capacity					1295:1302	good absorption capacity	1279:1302	good absorption capacity	1279:1302	Besides, owing to its low cost, good absorption capacity, and excellent reusability, this cryogel has potential for spill cleanup of oils and organic solvents.
33090346	2	31	theme	CF	391:392	arg1	waste					400:404	CF and C waste	391:404	waste	400:404	The keratin/cellulose-based composites were obtained by combining the dissolution of CF and C waste in 1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent via regeneration, simply by the freeze-drying method.
33090346	2	32	from	dissolution	376:386	arg1	solvent					476:482	1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent	409:482	1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent	409:482	The keratin/cellulose-based composites were obtained by combining the dissolution of CF and C waste in 1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent via regeneration, simply by the freeze-drying method.
33090346	3	33	theme	synthesized	575:585	arg1	composites					611:620	the synthesized keratin/cellulose-based composites	571:620	the synthesized keratin/cellulose-based composites	571:620	The characterization analysis of the synthesized keratin/cellulose-based composites was performed using Fourier transform infrared spectrometry, X-ray diffractometry, scanning electron microscopy, and thermogravimetry.
33090346	2	34	theme	Bmim-Cl+	447:454	arg1	solvent					476:482	1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent	409:482	1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent	409:482	The keratin/cellulose-based composites were obtained by combining the dissolution of CF and C waste in 1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent via regeneration, simply by the freeze-drying method.
33090346	8	35	theme	pseudo-second-order	1142:1160	arg1	model					1162:1166	the pseudo-second-order model	1138:1166	the pseudo-second-order model	1138:1166	Through the kinetic analysis, it was found that the pseudo-second-order model was more appropriate for the keratin/cellulose cryogel oil absorption process.
33090346	8	35	theme	pseudo-second-order	1142:1160	arg1	appropriate					1177:1187	appropriate	1177:1187	appropriate	1177:1187	Through the kinetic analysis, it was found that the pseudo-second-order model was more appropriate for the keratin/cellulose cryogel oil absorption process.
33090346	3	36	theme	scanning	705:712	arg1	microscopy					723:732	scanning electron microscopy	705:732	scanning electron microscopy	705:732	The characterization analysis of the synthesized keratin/cellulose-based composites was performed using Fourier transform infrared spectrometry, X-ray diffractometry, scanning electron microscopy, and thermogravimetry.
33090346	1	37	theme	Keratin/cellulose	116:132	arg1	cryogels					134:141	Keratin/cellulose cryogels	116:141	Keratin/cellulose cryogels	116:141	Keratin/cellulose cryogels were successfully fabricated using chicken feathers (CF) and cardboard (C) from environmental waste for the first time, to be exploited in oil/solvent absorption.
33090346	2	38	theme	chloride	437:444	arg1	solvent					476:482	1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent	409:482	1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent	409:482	The keratin/cellulose-based composites were obtained by combining the dissolution of CF and C waste in 1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent via regeneration, simply by the freeze-drying method.
33090346	0	39	theme	keratin/cellulose-based	36:58	arg1	composites					60:69	novel and ecological keratin/cellulose-based composites	15:69	novel and ecological keratin/cellulose-based composites	15:69	Development of novel and ecological keratin/cellulose-based composites for absorption of oils and organic solvents.
33090346	1	40	theme	oil/solvent	282:292	arg1	absorption					294:303	oil/solvent absorption	282:303	oil/solvent absorption	282:303	Keratin/cellulose cryogels were successfully fabricated using chicken feathers (CF) and cardboard (C) from environmental waste for the first time, to be exploited in oil/solvent absorption.
33090346	3	41	theme	electron	714:721	arg1	microscopy					723:732	scanning electron microscopy	705:732	scanning electron microscopy	705:732	The characterization analysis of the synthesized keratin/cellulose-based composites was performed using Fourier transform infrared spectrometry, X-ray diffractometry, scanning electron microscopy, and thermogravimetry.
33090346	3	42	dep	transform	650:658	arg1	infrared					660:667	infrared	660:667	transform infrared spectrometry, X-ray diffractometry, scanning electron microscopy, and thermogravimetry	650:754	The characterization analysis of the synthesized keratin/cellulose-based composites was performed using Fourier transform infrared spectrometry, X-ray diffractometry, scanning electron microscopy, and thermogravimetry.
33090346	2	43	theme	1-butyl-3-methylimidazolium	409:435	arg1	solvent					476:482	1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent	409:482	1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent	409:482	The keratin/cellulose-based composites were obtained by combining the dissolution of CF and C waste in 1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent via regeneration, simply by the freeze-drying method.
33090346	0	44	theme	ecological	25:34	arg1	composites					60:69	novel and ecological keratin/cellulose-based composites	15:69	novel and ecological keratin/cellulose-based composites	15:69	Development of novel and ecological keratin/cellulose-based composites for absorption of oils and organic solvents.
33090346	8	45	theme	absorption	1227:1236	arg1	process					1238:1244	the keratin/cellulose cryogel oil absorption process	1193:1244	the keratin/cellulose cryogel oil absorption process	1193:1244	Through the kinetic analysis, it was found that the pseudo-second-order model was more appropriate for the keratin/cellulose cryogel oil absorption process.
33090346	5	46	theme	initial	908:914	arg1	cryogel					916:922	the initial cryogel	904:922	the initial cryogel	904:922	Moreover, its sorption capacity can reach up to 6.9-17.7 times the weight of the initial cryogel.
33090346	0	47	theme	composites	60:69	arg1	Development					0:10	Development	0:10	Development of novel and ecological keratin/cellulose-based composites for absorption of oils and organic solvents.	0:114	Development of novel and ecological keratin/cellulose-based composites for absorption of oils and organic solvents.
33090346	2	48	theme	green	470:474	arg1	solvent					476:482	1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent	409:482	1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent	409:482	The keratin/cellulose-based composites were obtained by combining the dissolution of CF and C waste in 1-butyl-3-methylimidazolium chloride (Bmim-Cl+) ionic liquid green solvent via regeneration, simply by the freeze-drying method.
33090346	9	49	theme	excellent	1309:1317	arg1	reusability					1319:1329	excellent reusability	1309:1329	excellent reusability	1309:1329	Besides, owing to its low cost, good absorption capacity, and excellent reusability, this cryogel has potential for spill cleanup of oils and organic solvents.
33090346	3	50	theme	composites	611:620	arg1	analysis					559:566	The characterization analysis	538:566	The characterization analysis of the synthesized keratin/cellulose-based composites	538:620	The characterization analysis of the synthesized keratin/cellulose-based composites was performed using Fourier transform infrared spectrometry, X-ray diffractometry, scanning electron microscopy, and thermogravimetry.
33090346	8	51	theme	keratin/cellulose	1197:1213	arg1	absorption					1227:1236	keratin/cellulose cryogel oil absorption	1197:1236	the keratin/cellulose cryogel oil absorption process	1193:1244	Through the kinetic analysis, it was found that the pseudo-second-order model was more appropriate for the keratin/cellulose cryogel oil absorption process.
33090346	1	52	theme	environmental	223:235	arg1	waste					237:241	environmental waste	223:241	environmental waste	223:241	Keratin/cellulose cryogels were successfully fabricated using chicken feathers (CF) and cardboard (C) from environmental waste for the first time, to be exploited in oil/solvent absorption.
33090346	3	53	theme	X-ray	683:687	arg1	diffractometry					689:702	X-ray diffractometry	683:702	X-ray diffractometry	683:702	The characterization analysis of the synthesized keratin/cellulose-based composites was performed using Fourier transform infrared spectrometry, X-ray diffractometry, scanning electron microscopy, and thermogravimetry.
33090346	4	54	theme	various	792:798	arg1	oils					800:803	various oils	792:803	various oils	792:803	The as-prepared cryogel can absorb various oils and organic solvents.
33090346	8	55	theme	oil	1223:1225	arg1	absorption					1227:1236	keratin/cellulose cryogel oil absorption	1197:1236	the keratin/cellulose cryogel oil absorption process	1193:1244	Through the kinetic analysis, it was found that the pseudo-second-order model was more appropriate for the keratin/cellulose cryogel oil absorption process.
33090346	9	56	theme	organic	1389:1395	arg1	solvents					1397:1404	organic solvents	1389:1404	organic solvents	1389:1404	Besides, owing to its low cost, good absorption capacity, and excellent reusability, this cryogel has potential for spill cleanup of oils and organic solvents.
33090346	7	57	theme	absorption/desorption	1059:1079	arg1	methods					1081:1087	simple absorption/distillation and absorption/desorption methods	1024:1087	simple absorption/distillation and absorption/desorption methods	1024:1087	It could also be reused by simple absorption/distillation and absorption/desorption methods.
33090346	8	58	theme	cryogel	1215:1221	arg1	absorption					1227:1236	keratin/cellulose cryogel oil absorption	1197:1236	the keratin/cellulose cryogel oil absorption process	1193:1244	Through the kinetic analysis, it was found that the pseudo-second-order model was more appropriate for the keratin/cellulose cryogel oil absorption process.
33090346	6	59	theme	absorption	974:983	arg1	efficiency					985:994	good and fast absorption efficiency	960:994	good and fast absorption efficiency	960:994	This kind of CF/C cryogel revealed good and fast absorption efficiency.
33090346	0	60	theme	novel	15:19	arg1	composites					60:69	novel and ecological keratin/cellulose-based composites	15:69	novel and ecological keratin/cellulose-based composites	15:69	Development of novel and ecological keratin/cellulose-based composites for absorption of oils and organic solvents.
33090346	7	61	theme	simple	1024:1029	arg1	methods					1081:1087	simple absorption/distillation and absorption/desorption methods	1024:1087	simple absorption/distillation and absorption/desorption methods	1024:1087	It could also be reused by simple absorption/distillation and absorption/desorption methods.
33090346	1	62	theme	chicken	178:184	arg1	CF					196:197	CF	196:197	CF	196:197	Keratin/cellulose cryogels were successfully fabricated using chicken feathers (CF) and cardboard (C) from environmental waste for the first time, to be exploited in oil/solvent absorption.
33090346	1	62	theme	chicken	178:184	arg1	feathers					186:193	chicken feathers	178:193	chicken feathers (CF)	178:198	Keratin/cellulose cryogels were successfully fabricated using chicken feathers (CF) and cardboard (C) from environmental waste for the first time, to be exploited in oil/solvent absorption.
33090346	9	63	contain	has	1345:1347	arg2	potential					1349:1357	potential	1349:1357	potential for spill cleanup of oils and organic solvents	1349:1404	Besides, owing to its low cost, good absorption capacity, and excellent reusability, this cryogel has potential for spill cleanup of oils and organic solvents.
33090346	9	63	contain	has	1345:1347	arg1	cryogel					1337:1343	this cryogel	1332:1343	this cryogel	1332:1343	Besides, owing to its low cost, good absorption capacity, and excellent reusability, this cryogel has potential for spill cleanup of oils and organic solvents.
33992776	4	0	theme	GAG	619:621	arg1	saccharides					623:633	GAG saccharides	619:633	GAG saccharides	619:633	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	7	1	theme	PageRank	1189:1196	arg1	algorithm					1198:1206	Google's PageRank algorithm	1180:1206	Google's PageRank algorithm for ranking websites for search engine output	1180:1252	GAGrank is based on Google's PageRank algorithm for ranking websites for search engine output.
33992776	9	2	theme	tandem	1465:1470	arg1	fragments					1475:1483	the tandem MS fragments	1461:1483	the tandem MS fragments found using GAGfinder	1461:1505	In our implementation, the two partitions comprise every possible sequence for a given GAG composition and the tandem MS fragments found using GAGfinder.
33992776	2	3	theme	disaccharide	273:284	arg1	units					286:290	disaccharide units	273:290	disaccharide units with variable sulfation and acetylation patterns along the chain	273:355	These GAGs are characterized by repeating disaccharide units with variable sulfation and acetylation patterns along the chain.
33992776	4	4	theme	mass	699:702	arg1	spectra					704:710	the resulting complex tandem mass spectra	670:710	the resulting complex tandem mass spectra	670:710	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	11	5	theme	probabilistic	1617:1629	arg1	technique					1644:1652	the simulated annealing probabilistic optimization technique	1593:1652	the simulated annealing probabilistic optimization technique	1593:1652	Using the simulated annealing probabilistic optimization technique, we optimized GAGrank's parameters on ten training sequences.
33992776	5	6	theme	elemental	911:919	arg1	composition					921:931	elemental composition	911:931	elemental composition	911:931	We have recently published GAGfinder, the first peak picking and elemental composition assignment algorithm specifically designed for GAG tandem mass spectra.
33992776	11	7	theme	simulated	1597:1605	arg1	technique					1644:1652	the simulated annealing probabilistic optimization technique	1593:1652	the simulated annealing probabilistic optimization technique	1593:1652	Using the simulated annealing probabilistic optimization technique, we optimized GAGrank's parameters on ten training sequences.
33992776	5	8	theme	assignment	933:942	arg1	algorithm					944:952	the first peak picking and elemental composition assignment algorithm	884:952	the first peak picking and elemental composition assignment algorithm specifically designed for GAG tandem mass spectra	884:1002	We have recently published GAGfinder, the first peak picking and elemental composition assignment algorithm specifically designed for GAG tandem mass spectra.
33992776	5	8	theme	assignment	933:942	arg1	GAGfinder					873:881	GAGfinder	873:881	GAGfinder	873:881	We have recently published GAGfinder, the first peak picking and elemental composition assignment algorithm specifically designed for GAG tandem mass spectra.
33992776	13	9	theme	isomeric	1838:1845	arg1	mixtures					1847:1854	sequence isomeric mixtures	1829:1854	sequence isomeric mixtures	1829:1854	We also demonstrated GAGrank's ability to sequence isomeric mixtures using two mixtures at five different ratios.
33992776	5	10	theme	first	888:892	arg1	picking					899:905	first peak picking	888:905	first peak picking	888:905	We have recently published GAGfinder, the first peak picking and elemental composition assignment algorithm specifically designed for GAG tandem mass spectra.
33992776	4	11	theme	complex	684:690	arg1	spectra					704:710	the resulting complex tandem mass spectra	670:710	the resulting complex tandem mass spectra	670:710	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	5	12	theme	picking	899:905	arg1	algorithm					944:952	the first peak picking and elemental composition assignment algorithm	884:952	the first peak picking and elemental composition assignment algorithm specifically designed for GAG tandem mass spectra	884:1002	We have recently published GAGfinder, the first peak picking and elemental composition assignment algorithm specifically designed for GAG tandem mass spectra.
33992776	5	12	theme	picking	899:905	arg1	GAGfinder					873:881	GAGfinder	873:881	GAGfinder	873:881	We have recently published GAGfinder, the first peak picking and elemental composition assignment algorithm specifically designed for GAG tandem mass spectra.
33992776	5	13	theme	tandem	984:989	arg1	spectra					996:1002	GAG tandem mass spectra	980:1002	GAG tandem mass spectra	980:1002	We have recently published GAGfinder, the first peak picking and elemental composition assignment algorithm specifically designed for GAG tandem mass spectra.
33992776	1	14	dep	long	131:134	arg1	linear					137:142	linear	137:142	linear	137:142	The sulfated glycosaminoglycans (GAGs) are long, linear polysaccharide chains that are typically found as the glycan portion of proteoglycans.
33992776	12	15	from	performance	1744:1754	arg1	sequences					1776:1784	three validation sequences	1759:1784	three validation sequences	1759:1784	We then validated GAGrank's performance on three validation sequences.
33992776	4	16	theme	difficult	717:725	arg1	process					746:752	a difficult and time-consuming process	715:752	a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing	715:843	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	4	16	theme	difficult	717:725	arg1	interpretation					652:665	manual interpretation	645:665	manual interpretation of the resulting complex tandem mass spectra	645:710	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	4	17	theme	tandem	532:537	arg1	technique					577:585	a very effective technique	560:585	a very effective technique for assigning the structures of GAG saccharides	560:633	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	4	17	theme	tandem	532:537	arg1	spectrometry					544:555	Electron activated dissociation tandem mass spectrometry	500:555	Electron activated dissociation tandem mass spectrometry	500:555	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	4	18	theme	time-consuming	731:744	arg1	process					746:752	a difficult and time-consuming process	715:752	a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing	715:843	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	4	18	theme	time-consuming	731:744	arg1	interpretation					652:665	manual interpretation	645:665	manual interpretation of the resulting complex tandem mass spectra	645:710	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	0	19	theme	Graph	75:79	arg1	Model					81:85	a Bipartite Graph Model	63:85	a Bipartite Graph Model	63:85	GAGrank: Software for Glycosaminoglycan Sequence Ranking Using a Bipartite Graph Model.
33992776	1	20	theme	polysaccharide	144:157	arg1	chains					159:164	long, linear polysaccharide chains	131:164	long, linear polysaccharide chains that are typically found as the glycan portion of proteoglycans	131:228	The sulfated glycosaminoglycans (GAGs) are long, linear polysaccharide chains that are typically found as the glycan portion of proteoglycans.
33992776	1	20	theme	polysaccharide	144:157	arg1	portion					205:211	the glycan portion	194:211	the glycan portion of proteoglycans	194:228	The sulfated glycosaminoglycans (GAGs) are long, linear polysaccharide chains that are typically found as the glycan portion of proteoglycans.
33992776	1	20	theme	polysaccharide	144:157	arg1	glycosaminoglycans					101:118	The sulfated glycosaminoglycans	88:118	The sulfated glycosaminoglycans (GAGs)	88:125	The sulfated glycosaminoglycans (GAGs) are long, linear polysaccharide chains that are typically found as the glycan portion of proteoglycans.
33992776	3	21	theme	GAG	358:360	arg1	length					362:367	GAG length and modification patterns	358:393	length	362:367	GAG length and modification patterns have profound impacts on growth factor signaling mechanisms central to numerous physiological processes.
33992776	4	22	theme	accurate	811:818	arg1	sequencing					834:843	accurate and efficient sequencing	811:843	accurate and efficient sequencing	811:843	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	6	23	theme	novel	1033:1037	arg1	method					1053:1058	a novel network-based method	1031:1058	a novel network-based method for determining GAG structure using information extracted from tandem mass spectra using GAGfinder	1031:1157	Here, we present GAGrank, a novel network-based method for determining GAG structure using information extracted from tandem mass spectra using GAGfinder.
33992776	6	23	theme	novel	1033:1037	arg1	GAGrank					1022:1028	GAGrank	1022:1028	GAGrank	1022:1028	Here, we present GAGrank, a novel network-based method for determining GAG structure using information extracted from tandem mass spectra using GAGfinder.
33992776	4	24	theme	efficient	824:832	arg1	sequencing					834:843	accurate and efficient sequencing	811:843	accurate and efficient sequencing	811:843	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	0	25	theme	Sequence	40:47	arg1	Ranking					49:55	Glycosaminoglycan Sequence Ranking	22:55	Glycosaminoglycan Sequence Ranking Using a Bipartite Graph Model	22:85	GAGrank: Software for Glycosaminoglycan Sequence Ranking Using a Bipartite Graph Model.
33992776	4	26	theme	activated	509:517	arg1	technique					577:585	a very effective technique	560:585	a very effective technique for assigning the structures of GAG saccharides	560:633	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	4	26	theme	activated	509:517	arg1	spectrometry					544:555	Electron activated dissociation tandem mass spectrometry	500:555	Electron activated dissociation tandem mass spectrometry	500:555	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	3	27	theme	factor	427:432	arg1	mechanisms					444:453	growth factor signaling mechanisms	420:453	growth factor signaling mechanisms central to numerous physiological processes	420:497	GAG length and modification patterns have profound impacts on growth factor signaling mechanisms central to numerous physiological processes.
33992776	6	28	theme	mass	1130:1133	arg1	spectra					1135:1141	tandem mass spectra	1123:1141	tandem mass spectra using GAGfinder	1123:1157	Here, we present GAGrank, a novel network-based method for determining GAG structure using information extracted from tandem mass spectra using GAGfinder.
33992776	4	29	theme	methods	799:805	arg1	development					770:780	the development	766:780	the development of computational methods for accurate and efficient sequencing	766:843	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	3	30	theme	physiological	475:487	arg1	processes					489:497	numerous physiological processes	466:497	numerous physiological processes	466:497	GAG length and modification patterns have profound impacts on growth factor signaling mechanisms central to numerous physiological processes.
33992776	7	31	theme	search	1233:1238	arg1	engine					1240:1245	search engine	1233:1245	search engine output	1233:1252	GAGrank is based on Google's PageRank algorithm for ranking websites for search engine output.
33992776	8	32	theme	BiRank	1297:1302	arg1	it					1270:1271	it	1270:1271	it	1270:1271	In particular, it is an implementation of BiRank, an extension of PageRank for bipartite networks.
33992776	8	32	theme	BiRank	1297:1302	arg1	implementation					1279:1292	an implementation	1276:1292	an implementation of BiRank, an extension of PageRank for bipartite networks	1276:1351	In particular, it is an implementation of BiRank, an extension of PageRank for bipartite networks.
33992776	9	33	theme	possible	1411:1418	arg1	sequence					1420:1427	every possible sequence	1405:1427	every possible sequence for a given GAG composition and the tandem MS fragments found using GAGfinder	1405:1505	In our implementation, the two partitions comprise every possible sequence for a given GAG composition and the tandem MS fragments found using GAGfinder.
33992776	0	34	theme	Glycosaminoglycan	22:38	arg1	Ranking					49:55	Glycosaminoglycan Sequence Ranking	22:55	Glycosaminoglycan Sequence Ranking Using a Bipartite Graph Model	22:85	GAGrank: Software for Glycosaminoglycan Sequence Ranking Using a Bipartite Graph Model.
33992776	2	35	with	units	286:290	arg1	sulfation					306:314	variable sulfation and acetylation patterns	297:339	sulfation	306:314	These GAGs are characterized by repeating disaccharide units with variable sulfation and acetylation patterns along the chain.
33992776	2	35	with	units	286:290	arg1	patterns					332:339	variable sulfation and acetylation patterns	297:339	patterns	332:339	These GAGs are characterized by repeating disaccharide units with variable sulfation and acetylation patterns along the chain.
33992776	3	36	theme	profound	400:407	arg1	impacts					409:415	profound impacts	400:415	profound impacts	400:415	GAG length and modification patterns have profound impacts on growth factor signaling mechanisms central to numerous physiological processes.
33992776	10	37	theme	important	1566:1574	arg1	fragments					1576:1584	many important fragments	1561:1584	many important fragments	1561:1584	Sequences are given a higher ranking if they link to many important fragments.
33992776	4	38	theme	effective	567:575	arg1	technique					577:585	a very effective technique	560:585	a very effective technique for assigning the structures of GAG saccharides	560:633	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	4	38	theme	effective	567:575	arg1	spectrometry					544:555	Electron activated dissociation tandem mass spectrometry	500:555	Electron activated dissociation tandem mass spectrometry	500:555	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	10	39	attach	link	1553:1556	arg1	fragments					1576:1584	many important fragments	1561:1584	many important fragments	1561:1584	Sequences are given a higher ranking if they link to many important fragments.
33992776	10	39	attach	link	1553:1556	arg2	they					1548:1551	they	1548:1551	they	1548:1551	Sequences are given a higher ranking if they link to many important fragments.
33992776	9	40	theme	given	1435:1439	arg1	composition					1445:1455	a given GAG composition	1433:1455	a given GAG composition	1433:1455	In our implementation, the two partitions comprise every possible sequence for a given GAG composition and the tandem MS fragments found using GAGfinder.
33992776	1	41	theme	proteoglycans	216:228	arg1	portion					205:211	the glycan portion	194:211	the glycan portion of proteoglycans	194:228	The sulfated glycosaminoglycans (GAGs) are long, linear polysaccharide chains that are typically found as the glycan portion of proteoglycans.
33992776	1	41	theme	proteoglycans	216:228	arg1	chains					159:164	long, linear polysaccharide chains	131:164	long, linear polysaccharide chains that are typically found as the glycan portion of proteoglycans	131:228	The sulfated glycosaminoglycans (GAGs) are long, linear polysaccharide chains that are typically found as the glycan portion of proteoglycans.
33992776	0	42	dep	GAGrank	0:6	arg1	Software					9:16	Software	9:16	GAGrank: Software for Glycosaminoglycan Sequence Ranking Using a Bipartite Graph Model.	0:86	GAGrank: Software for Glycosaminoglycan Sequence Ranking Using a Bipartite Graph Model.
33992776	4	43	theme	manual	645:650	arg1	process					746:752	a difficult and time-consuming process	715:752	a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing	715:843	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	4	43	theme	manual	645:650	arg1	interpretation					652:665	manual interpretation	645:665	manual interpretation of the resulting complex tandem mass spectra	645:710	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	13	44	theme	different	1883:1891	arg1	ratios					1893:1898	five different ratios	1878:1898	five different ratios	1878:1898	We also demonstrated GAGrank's ability to sequence isomeric mixtures using two mixtures at five different ratios.
33992776	11	45	theme	training	1696:1703	arg1	sequences					1705:1713	ten training sequences	1692:1713	ten training sequences	1692:1713	Using the simulated annealing probabilistic optimization technique, we optimized GAGrank's parameters on ten training sequences.
33992776	9	46	theme	MS	1472:1473	arg1	fragments					1475:1483	the tandem MS fragments	1461:1483	the tandem MS fragments found using GAGfinder	1461:1505	In our implementation, the two partitions comprise every possible sequence for a given GAG composition and the tandem MS fragments found using GAGfinder.
33992776	8	47	from	implementation	1279:1292	arg1	particular					1258:1267	particular	1258:1267	particular	1258:1267	In particular, it is an implementation of BiRank, an extension of PageRank for bipartite networks.
33992776	4	48	theme	saccharides	623:633	arg1	structures					605:614	the structures	601:614	the structures of GAG saccharides	601:633	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	4	49	theme	tandem	692:697	arg1	spectra					704:710	the resulting complex tandem mass spectra	670:710	the resulting complex tandem mass spectra	670:710	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	11	50	theme	optimization	1631:1642	arg1	technique					1644:1652	the simulated annealing probabilistic optimization technique	1593:1652	the simulated annealing probabilistic optimization technique	1593:1652	Using the simulated annealing probabilistic optimization technique, we optimized GAGrank's parameters on ten training sequences.
33992776	5	51	theme	composition	921:931	arg1	algorithm					944:952	the first peak picking and elemental composition assignment algorithm	884:952	the first peak picking and elemental composition assignment algorithm specifically designed for GAG tandem mass spectra	884:1002	We have recently published GAGfinder, the first peak picking and elemental composition assignment algorithm specifically designed for GAG tandem mass spectra.
33992776	5	51	theme	composition	921:931	arg1	GAGfinder					873:881	GAGfinder	873:881	GAGfinder	873:881	We have recently published GAGfinder, the first peak picking and elemental composition assignment algorithm specifically designed for GAG tandem mass spectra.
33992776	3	52	contain	have	395:398	arg1	patterns					386:393	GAG length and modification patterns	358:393	patterns	386:393	GAG length and modification patterns have profound impacts on growth factor signaling mechanisms central to numerous physiological processes.
33992776	3	52	contain	have	395:398	arg2	impacts					409:415	profound impacts	400:415	profound impacts	400:415	GAG length and modification patterns have profound impacts on growth factor signaling mechanisms central to numerous physiological processes.
33992776	3	52	contain	have	395:398	arg1	length					362:367	GAG length and modification patterns	358:393	length	362:367	GAG length and modification patterns have profound impacts on growth factor signaling mechanisms central to numerous physiological processes.
33992776	4	53	theme	spectra	704:710	arg1	process					746:752	a difficult and time-consuming process	715:752	a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing	715:843	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	4	53	theme	spectra	704:710	arg1	interpretation					652:665	manual interpretation	645:665	manual interpretation of the resulting complex tandem mass spectra	645:710	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	13	54	theme	sequence	1829:1836	arg1	mixtures					1847:1854	sequence isomeric mixtures	1829:1854	sequence isomeric mixtures	1829:1854	We also demonstrated GAGrank's ability to sequence isomeric mixtures using two mixtures at five different ratios.
33992776	11	55	theme	annealing	1607:1615	arg1	technique					1644:1652	the simulated annealing probabilistic optimization technique	1593:1652	the simulated annealing probabilistic optimization technique	1593:1652	Using the simulated annealing probabilistic optimization technique, we optimized GAGrank's parameters on ten training sequences.
33992776	2	56	theme	variable	297:304	arg1	sulfation					306:314	variable sulfation and acetylation patterns	297:339	sulfation	306:314	These GAGs are characterized by repeating disaccharide units with variable sulfation and acetylation patterns along the chain.
33992776	5	57	theme	peak	894:897	arg1	picking					899:905	first peak picking	888:905	first peak picking	888:905	We have recently published GAGfinder, the first peak picking and elemental composition assignment algorithm specifically designed for GAG tandem mass spectra.
33992776	4	58	theme	resulting	674:682	arg1	spectra					704:710	the resulting complex tandem mass spectra	670:710	the resulting complex tandem mass spectra	670:710	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	5	59	theme	GAG	980:982	arg1	spectra					996:1002	GAG tandem mass spectra	980:1002	GAG tandem mass spectra	980:1002	We have recently published GAGfinder, the first peak picking and elemental composition assignment algorithm specifically designed for GAG tandem mass spectra.
33992776	8	60	theme	bipartite	1334:1342	arg1	networks					1344:1351	bipartite networks	1334:1351	bipartite networks	1334:1351	In particular, it is an implementation of BiRank, an extension of PageRank for bipartite networks.
33992776	5	61	theme	mass	991:994	arg1	spectra					996:1002	GAG tandem mass spectra	980:1002	GAG tandem mass spectra	980:1002	We have recently published GAGfinder, the first peak picking and elemental composition assignment algorithm specifically designed for GAG tandem mass spectra.
33992776	12	62	theme	validation	1765:1774	arg1	sequences					1776:1784	three validation sequences	1759:1784	three validation sequences	1759:1784	We then validated GAGrank's performance on three validation sequences.
33992776	0	63	theme	Bipartite	65:73	arg1	Model					81:85	a Bipartite Graph Model	63:85	a Bipartite Graph Model	63:85	GAGrank: Software for Glycosaminoglycan Sequence Ranking Using a Bipartite Graph Model.
33992776	1	64	theme	long	131:134	arg1	chains					159:164	long, linear polysaccharide chains	131:164	long, linear polysaccharide chains that are typically found as the glycan portion of proteoglycans	131:228	The sulfated glycosaminoglycans (GAGs) are long, linear polysaccharide chains that are typically found as the glycan portion of proteoglycans.
33992776	1	64	theme	long	131:134	arg1	portion					205:211	the glycan portion	194:211	the glycan portion of proteoglycans	194:228	The sulfated glycosaminoglycans (GAGs) are long, linear polysaccharide chains that are typically found as the glycan portion of proteoglycans.
33992776	1	64	theme	long	131:134	arg1	glycosaminoglycans					101:118	The sulfated glycosaminoglycans	88:118	The sulfated glycosaminoglycans (GAGs)	88:125	The sulfated glycosaminoglycans (GAGs) are long, linear polysaccharide chains that are typically found as the glycan portion of proteoglycans.
33992776	6	65	theme	GAG	1076:1078	arg1	structure					1080:1088	GAG structure	1076:1088	GAG structure using information extracted from tandem mass spectra using GAGfinder	1076:1157	Here, we present GAGrank, a novel network-based method for determining GAG structure using information extracted from tandem mass spectra using GAGfinder.
33992776	4	66	theme	mass	539:542	arg1	technique					577:585	a very effective technique	560:585	a very effective technique for assigning the structures of GAG saccharides	560:633	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	4	66	theme	mass	539:542	arg1	spectrometry					544:555	Electron activated dissociation tandem mass spectrometry	500:555	Electron activated dissociation tandem mass spectrometry	500:555	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	13	67	dep	ability	1818:1824	arg1	using					1856:1860	using	1856:1860	using two mixtures at five different ratios	1856:1898	We also demonstrated GAGrank's ability to sequence isomeric mixtures using two mixtures at five different ratios.
33992776	6	68	theme	network-based	1039:1051	arg1	method					1053:1058	a novel network-based method	1031:1058	a novel network-based method for determining GAG structure using information extracted from tandem mass spectra using GAGfinder	1031:1157	Here, we present GAGrank, a novel network-based method for determining GAG structure using information extracted from tandem mass spectra using GAGfinder.
33992776	6	68	theme	network-based	1039:1051	arg1	GAGrank					1022:1028	GAGrank	1022:1028	GAGrank	1022:1028	Here, we present GAGrank, a novel network-based method for determining GAG structure using information extracted from tandem mass spectra using GAGfinder.
33992776	4	69	theme	Electron	500:507	arg1	technique					577:585	a very effective technique	560:585	a very effective technique for assigning the structures of GAG saccharides	560:633	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	4	69	theme	Electron	500:507	arg1	spectrometry					544:555	Electron activated dissociation tandem mass spectrometry	500:555	Electron activated dissociation tandem mass spectrometry	500:555	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	4	70	theme	dissociation	519:530	arg1	technique					577:585	a very effective technique	560:585	a very effective technique for assigning the structures of GAG saccharides	560:633	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	4	70	theme	dissociation	519:530	arg1	spectrometry					544:555	Electron activated dissociation tandem mass spectrometry	500:555	Electron activated dissociation tandem mass spectrometry	500:555	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	3	71	theme	central	455:461	arg1	mechanisms					444:453	growth factor signaling mechanisms	420:453	growth factor signaling mechanisms central to numerous physiological processes	420:497	GAG length and modification patterns have profound impacts on growth factor signaling mechanisms central to numerous physiological processes.
33992776	7	72	theme	engine	1240:1245	arg1	output					1247:1252	search engine output	1233:1252	search engine output	1233:1252	GAGrank is based on Google's PageRank algorithm for ranking websites for search engine output.
33992776	3	73	theme	signaling	434:442	arg1	mechanisms					444:453	growth factor signaling mechanisms	420:453	growth factor signaling mechanisms central to numerous physiological processes	420:497	GAG length and modification patterns have profound impacts on growth factor signaling mechanisms central to numerous physiological processes.
33992776	1	74	theme	sulfated	92:99	arg1	chains					159:164	long, linear polysaccharide chains	131:164	long, linear polysaccharide chains that are typically found as the glycan portion of proteoglycans	131:228	The sulfated glycosaminoglycans (GAGs) are long, linear polysaccharide chains that are typically found as the glycan portion of proteoglycans.
33992776	1	74	theme	sulfated	92:99	arg1	GAGs					121:124	GAGs	121:124	GAGs	121:124	The sulfated glycosaminoglycans (GAGs) are long, linear polysaccharide chains that are typically found as the glycan portion of proteoglycans.
33992776	1	74	theme	sulfated	92:99	arg1	glycosaminoglycans					101:118	The sulfated glycosaminoglycans	88:118	The sulfated glycosaminoglycans (GAGs)	88:125	The sulfated glycosaminoglycans (GAGs) are long, linear polysaccharide chains that are typically found as the glycan portion of proteoglycans.
33992776	6	75	theme	tandem	1123:1128	arg1	spectra					1135:1141	tandem mass spectra	1123:1141	tandem mass spectra using GAGfinder	1123:1157	Here, we present GAGrank, a novel network-based method for determining GAG structure using information extracted from tandem mass spectra using GAGfinder.
33992776	3	76	theme	modification	373:384	arg1	patterns					386:393	GAG length and modification patterns	358:393	patterns	386:393	GAG length and modification patterns have profound impacts on growth factor signaling mechanisms central to numerous physiological processes.
33992776	4	77	theme	computational	785:797	arg1	methods					799:805	computational methods	785:805	computational methods for accurate and efficient sequencing	785:843	Electron activated dissociation tandem mass spectrometry is a very effective technique for assigning the structures of GAG saccharides; however, manual interpretation of the resulting complex tandem mass spectra is a difficult and time-consuming process that drives the development of computational methods for accurate and efficient sequencing.
33992776	7	78	theme	ranking	1212:1218	arg1	websites					1220:1227	ranking websites	1212:1227	ranking websites for search engine output	1212:1252	GAGrank is based on Google's PageRank algorithm for ranking websites for search engine output.
33992776	3	79	theme	growth	420:425	arg1	mechanisms					444:453	growth factor signaling mechanisms	420:453	growth factor signaling mechanisms central to numerous physiological processes	420:497	GAG length and modification patterns have profound impacts on growth factor signaling mechanisms central to numerous physiological processes.
33992776	10	80	theme	higher	1530:1535	arg1	ranking					1537:1543	a higher ranking	1528:1543	a higher ranking if they link to many important fragments	1528:1584	Sequences are given a higher ranking if they link to many important fragments.
33992776	3	81	theme	numerous	466:473	arg1	processes					489:497	numerous physiological processes	466:497	numerous physiological processes	466:497	GAG length and modification patterns have profound impacts on growth factor signaling mechanisms central to numerous physiological processes.
33992776	10	82	theme	many	1561:1564	arg1	fragments					1576:1584	many important fragments	1561:1584	many important fragments	1561:1584	Sequences are given a higher ranking if they link to many important fragments.
33992776	8	83	theme	PageRank	1321:1328	arg1	BiRank					1297:1302	BiRank	1297:1302	BiRank	1297:1302	In particular, it is an implementation of BiRank, an extension of PageRank for bipartite networks.
33992776	8	83	theme	PageRank	1321:1328	arg1	extension					1308:1316	an extension	1305:1316	an extension of PageRank for bipartite networks	1305:1351	In particular, it is an implementation of BiRank, an extension of PageRank for bipartite networks.
33992776	2	84	theme	acetylation	320:330	arg1	patterns					332:339	variable sulfation and acetylation patterns	297:339	patterns	332:339	These GAGs are characterized by repeating disaccharide units with variable sulfation and acetylation patterns along the chain.
33992776	9	85	theme	GAG	1441:1443	arg1	composition					1445:1455	a given GAG composition	1433:1455	a given GAG composition	1433:1455	In our implementation, the two partitions comprise every possible sequence for a given GAG composition and the tandem MS fragments found using GAGfinder.
33992776	1	86	theme	glycan	198:203	arg1	portion					205:211	the glycan portion	194:211	the glycan portion of proteoglycans	194:228	The sulfated glycosaminoglycans (GAGs) are long, linear polysaccharide chains that are typically found as the glycan portion of proteoglycans.
33992776	1	86	theme	glycan	198:203	arg1	chains					159:164	long, linear polysaccharide chains	131:164	long, linear polysaccharide chains that are typically found as the glycan portion of proteoglycans	131:228	The sulfated glycosaminoglycans (GAGs) are long, linear polysaccharide chains that are typically found as the glycan portion of proteoglycans.
33872613	7	0	theme	Chinese	1124:1130	arg1	medicine					1132:1139	a traditional Chinese medicine	1110:1139	a traditional Chinese medicine	1110:1139	Simultaneously, the discovery of ADP80-2 further revealed the chemical composition of A. dahurica used as a traditional Chinese medicine and spice.
33872613	4	1	theme	activity	498:505	arg1	terms					472:476	terms	472:476	terms of immunoregulatory activity	472:505	In terms of immunoregulatory activity, ADP80-2 can significantly promote the phagocytosis, the production of nitric oxide (NO), and the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage.
33872613	3	2	dep	-α-L-Araf-	369:378	arg1	5					388:388	5	388:388	5	388:388	The backbone of ADP80-2 comprised →5)-α-L-Araf-(1→, →3, 5)-α-L-Araf-(1→, →6)-α-D-Glcp-(1→, with a terminal branch α-L-Araf-(1 → residue.
33872613	3	2	dep	-α-L-Araf-	369:378	arg1	1→					419:420	-α-L-Araf-(1→, →6)-α-D-Glcp-(1→	390:420	1→	419:420	The backbone of ADP80-2 comprised →5)-α-L-Araf-(1→, →3, 5)-α-L-Araf-(1→, →6)-α-D-Glcp-(1→, with a terminal branch α-L-Araf-(1 → residue.
33872613	3	2	dep	-α-L-Araf-	369:378	arg1	→5					366:367	→5	366:367	→5	366:367	The backbone of ADP80-2 comprised →5)-α-L-Araf-(1→, →3, 5)-α-L-Araf-(1→, →6)-α-D-Glcp-(1→, with a terminal branch α-L-Araf-(1 → residue.
33872613	3	3	theme	terminal	430:437	arg1	residue					460:466	a terminal branch α-L-Araf-(1 → residue	428:466	a terminal branch α-L-Araf-(1 → residue	428:466	The backbone of ADP80-2 comprised →5)-α-L-Araf-(1→, →3, 5)-α-L-Araf-(1→, →6)-α-D-Glcp-(1→, with a terminal branch α-L-Araf-(1 → residue.
33872613	4	4	theme	immunoregulatory	481:496	arg1	activity					498:505	immunoregulatory activity	481:505	immunoregulatory activity	481:505	In terms of immunoregulatory activity, ADP80-2 can significantly promote the phagocytosis, the production of nitric oxide (NO), and the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage.
33872613	3	5	theme	→	458:458	arg1	residue					460:466	a terminal branch α-L-Araf-(1 → residue	428:466	a terminal branch α-L-Araf-(1 → residue	428:466	The backbone of ADP80-2 comprised →5)-α-L-Araf-(1→, →3, 5)-α-L-Araf-(1→, →6)-α-D-Glcp-(1→, with a terminal branch α-L-Araf-(1 → residue.
33872613	0	6	theme	gluco-arabinan	85:98	arg1	properties					69:78	immunoregulatory properties	52:78	Structural characteristics and in vitro and in vivo immunoregulatory properties of a gluco-arabinan from Angelica dahurica.	0:122	Structural characteristics and in vitro and in vivo immunoregulatory properties of a gluco-arabinan from Angelica dahurica.
33872613	3	7	dep	1→	419:420	arg1	→6					405:406	-α-L-Araf-(1→, →6)-α-D-Glcp-(1→	390:420	→6	405:406	The backbone of ADP80-2 comprised →5)-α-L-Araf-(1→, →3, 5)-α-L-Araf-(1→, →6)-α-D-Glcp-(1→, with a terminal branch α-L-Araf-(1 → residue.
33872613	2	8	theme	glucose	285:291	arg1	arabinose					260:268	arabinose	260:268	arabinose	260:268	ADP80-2 was a gluco-arabinan composed of arabinose and a trace of glucose with a molecular weight of 9950 g/mol.
33872613	2	8	theme	glucose	285:291	arg1	trace					276:280	a trace	274:280	a trace of glucose with a molecular weight of 9950 g/mol	274:329	ADP80-2 was a gluco-arabinan composed of arabinose and a trace of glucose with a molecular weight of 9950 g/mol.
33872613	4	9	theme	nitric	578:583	arg1	NO					592:593	NO	592:593	NO	592:593	In terms of immunoregulatory activity, ADP80-2 can significantly promote the phagocytosis, the production of nitric oxide (NO), and the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage.
33872613	4	9	theme	nitric	578:583	arg1	oxide					585:589	nitric oxide	578:589	nitric oxide (NO)	578:594	In terms of immunoregulatory activity, ADP80-2 can significantly promote the phagocytosis, the production of nitric oxide (NO), and the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage.
33872613	3	10	theme	ADP80-2	348:354	arg1	backbone					336:343	The backbone	332:343	The backbone of ADP80-2	332:354	The backbone of ADP80-2 comprised →5)-α-L-Araf-(1→, →3, 5)-α-L-Araf-(1→, →6)-α-D-Glcp-(1→, with a terminal branch α-L-Araf-(1 → residue.
33872613	6	11	theme	immunomodulatory	901:916	arg1	effects					918:924	immunomodulatory effects	901:924	immunomodulatory effects	901:924	These biological results indicated that ADP80-2 with immunomodulatory effects was expected to be useful for the development of new immunomodulatory agents.
33872613	7	12	theme	dahurica	1093:1100	arg1	composition					1075:1085	the chemical composition	1062:1085	the chemical composition of A. dahurica used as a traditional Chinese medicine and spice	1062:1149	Simultaneously, the discovery of ADP80-2 further revealed the chemical composition of A. dahurica used as a traditional Chinese medicine and spice.
33872613	6	13	theme	agents	996:1001	arg1	development					960:970	the development	956:970	the development of new immunomodulatory agents	956:1001	These biological results indicated that ADP80-2 with immunomodulatory effects was expected to be useful for the development of new immunomodulatory agents.
33872613	3	14	theme	1→	401:402	arg1	1→					419:420	-α-L-Araf-(1→, →6)-α-D-Glcp-(1→	390:420	1→	419:420	The backbone of ADP80-2 comprised →5)-α-L-Araf-(1→, →3, 5)-α-L-Araf-(1→, →6)-α-D-Glcp-(1→, with a terminal branch α-L-Araf-(1 → residue.
33872613	4	15	dep	cytokines	618:626	arg1	IL-1β					635:639	IL-1β	635:639	IL-1β	635:639	In terms of immunoregulatory activity, ADP80-2 can significantly promote the phagocytosis, the production of nitric oxide (NO), and the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage.
33872613	4	15	dep	cytokines	618:626	arg1	TNF-α					646:650	TNF-α	646:650	TNF-α	646:650	In terms of immunoregulatory activity, ADP80-2 can significantly promote the phagocytosis, the production of nitric oxide (NO), and the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage.
33872613	4	15	dep	cytokines	618:626	arg1	IL-6					629:632	IL-6	629:632	IL-6	629:632	In terms of immunoregulatory activity, ADP80-2 can significantly promote the phagocytosis, the production of nitric oxide (NO), and the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage.
33872613	4	15	dep	cytokines	618:626	arg1	cytokines					618:626	cytokines	618:626	cytokines (IL-6, IL-1β, and TNF-α)	618:651	In terms of immunoregulatory activity, ADP80-2 can significantly promote the phagocytosis, the production of nitric oxide (NO), and the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage.
33872613	0	16	theme	Structural	0:9	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics and in vitro and in vivo immunoregulatory properties of a gluco-arabinan from Angelica dahurica.	0:122	Structural characteristics and in vitro and in vivo immunoregulatory properties of a gluco-arabinan from Angelica dahurica.
33872613	3	17	theme	-α-L-Araf-	390:399	arg1	1→					419:420	-α-L-Araf-(1→, →6)-α-D-Glcp-(1→	390:420	1→	419:420	The backbone of ADP80-2 comprised →5)-α-L-Araf-(1→, →3, 5)-α-L-Araf-(1→, →6)-α-D-Glcp-(1→, with a terminal branch α-L-Araf-(1 → residue.
33872613	6	18	theme	new	975:977	arg1	agents					996:1001	new immunomodulatory agents	975:1001	new immunomodulatory agents	975:1001	These biological results indicated that ADP80-2 with immunomodulatory effects was expected to be useful for the development of new immunomodulatory agents.
33872613	5	19	theme	immunomodulatory	696:711	arg1	activities					713:722	the cellular immunomodulatory activities	683:722	the cellular immunomodulatory activities	683:722	In addition to the cellular immunomodulatory activities, the chemokines related to immunoregulation were significantly increased in the zebrafish model after treated with ADP80-2.
33872613	5	20	theme	cellular	687:694	arg1	activities					713:722	the cellular immunomodulatory activities	683:722	the cellular immunomodulatory activities	683:722	In addition to the cellular immunomodulatory activities, the chemokines related to immunoregulation were significantly increased in the zebrafish model after treated with ADP80-2.
33872613	6	21	theme	biological	854:863	arg1	results					865:871	These biological results	848:871	These biological results	848:871	These biological results indicated that ADP80-2 with immunomodulatory effects was expected to be useful for the development of new immunomodulatory agents.
33872613	2	22	theme	molecular	300:308	arg1	weight					310:315	a molecular weight	298:315	a molecular weight of 9950 g/mol	298:329	ADP80-2 was a gluco-arabinan composed of arabinose and a trace of glucose with a molecular weight of 9950 g/mol.
33872613	3	23	theme	-α-D-Glcp-	408:417	arg1	1→					419:420	-α-L-Araf-(1→, →6)-α-D-Glcp-(1→	390:420	1→	419:420	The backbone of ADP80-2 comprised →5)-α-L-Araf-(1→, →3, 5)-α-L-Araf-(1→, →6)-α-D-Glcp-(1→, with a terminal branch α-L-Araf-(1 → residue.
33872613	5	24	theme	zebrafish	804:812	arg1	model					814:818	the zebrafish model	800:818	the zebrafish model	800:818	In addition to the cellular immunomodulatory activities, the chemokines related to immunoregulation were significantly increased in the zebrafish model after treated with ADP80-2.
33872613	1	25	theme	Angelica	200:207	arg1	dahurica					209:216	Angelica dahurica	200:216	Angelica dahurica	200:216	A water-soluble polysaccharide identified here as ADP80-2 was acquired from Angelica dahurica.
33872613	6	26	theme	immunomodulatory	979:994	arg1	agents					996:1001	new immunomodulatory agents	975:1001	new immunomodulatory agents	975:1001	These biological results indicated that ADP80-2 with immunomodulatory effects was expected to be useful for the development of new immunomodulatory agents.
33872613	7	27	theme	chemical	1066:1073	arg1	composition					1075:1085	the chemical composition	1062:1085	the chemical composition of A. dahurica used as a traditional Chinese medicine and spice	1062:1149	Simultaneously, the discovery of ADP80-2 further revealed the chemical composition of A. dahurica used as a traditional Chinese medicine and spice.
33872613	0	28	from	properties	69:78	arg1	dahurica					114:121	dahurica	114:121	dahurica	114:121	Structural characteristics and in vitro and in vivo immunoregulatory properties of a gluco-arabinan from Angelica dahurica.
33872613	7	29	theme	ADP80-2	1037:1043	arg1	discovery					1024:1032	the discovery	1020:1032	the discovery of ADP80-2	1020:1043	Simultaneously, the discovery of ADP80-2 further revealed the chemical composition of A. dahurica used as a traditional Chinese medicine and spice.
33872613	2	30	with	arabinose	260:268	arg1	weight					310:315	a molecular weight	298:315	a molecular weight of 9950 g/mol	298:329	ADP80-2 was a gluco-arabinan composed of arabinose and a trace of glucose with a molecular weight of 9950 g/mol.
33872613	2	31	with	trace	276:280	arg1	weight					310:315	a molecular weight	298:315	a molecular weight of 9950 g/mol	298:329	ADP80-2 was a gluco-arabinan composed of arabinose and a trace of glucose with a molecular weight of 9950 g/mol.
33872613	0	32	from	dahurica	114:121	arg1	properties					69:78	immunoregulatory properties	52:78	Structural characteristics and in vitro and in vivo immunoregulatory properties of a gluco-arabinan from Angelica dahurica.	0:122	Structural characteristics and in vitro and in vivo immunoregulatory properties of a gluco-arabinan from Angelica dahurica.
33872613	0	32	from	dahurica	114:121	arg1	gluco-arabinan					85:98	a gluco-arabinan	83:98	a gluco-arabinan from Angelica dahurica	83:121	Structural characteristics and in vitro and in vivo immunoregulatory properties of a gluco-arabinan from Angelica dahurica.
33872613	4	33	theme	oxide	585:589	arg1	secretion					605:613	the secretion	601:613	the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage	601:665	In terms of immunoregulatory activity, ADP80-2 can significantly promote the phagocytosis, the production of nitric oxide (NO), and the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage.
33872613	4	33	theme	oxide	585:589	arg1	phagocytosis					546:557	the phagocytosis	542:557	the phagocytosis	542:557	In terms of immunoregulatory activity, ADP80-2 can significantly promote the phagocytosis, the production of nitric oxide (NO), and the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage.
33872613	4	33	theme	oxide	585:589	arg1	production					564:573	the production	560:573	the production of nitric oxide (NO)	560:594	In terms of immunoregulatory activity, ADP80-2 can significantly promote the phagocytosis, the production of nitric oxide (NO), and the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage.
33872613	6	34	with	ADP80-2	888:894	arg1	effects					918:924	immunomodulatory effects	901:924	immunomodulatory effects	901:924	These biological results indicated that ADP80-2 with immunomodulatory effects was expected to be useful for the development of new immunomodulatory agents.
33872613	4	35	theme	macrophage	656:665	arg1	secretion					605:613	the secretion	601:613	the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage	601:665	In terms of immunoregulatory activity, ADP80-2 can significantly promote the phagocytosis, the production of nitric oxide (NO), and the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage.
33872613	4	35	theme	macrophage	656:665	arg1	phagocytosis					546:557	the phagocytosis	542:557	the phagocytosis	542:557	In terms of immunoregulatory activity, ADP80-2 can significantly promote the phagocytosis, the production of nitric oxide (NO), and the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage.
33872613	4	35	theme	macrophage	656:665	arg1	production					564:573	the production	560:573	the production of nitric oxide (NO)	560:594	In terms of immunoregulatory activity, ADP80-2 can significantly promote the phagocytosis, the production of nitric oxide (NO), and the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage.
33872613	1	36	theme	water-soluble	126:138	arg1	polysaccharide					140:153	A water-soluble polysaccharide	124:153	A water-soluble polysaccharide identified here as ADP80-2	124:180	A water-soluble polysaccharide identified here as ADP80-2 was acquired from Angelica dahurica.
33872613	2	37	theme	g/mol	325:329	arg1	weight					310:315	a molecular weight	298:315	a molecular weight of 9950 g/mol	298:329	ADP80-2 was a gluco-arabinan composed of arabinose and a trace of glucose with a molecular weight of 9950 g/mol.
33872613	4	38	theme	cytokines	618:626	arg1	secretion					605:613	the secretion	601:613	the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage	601:665	In terms of immunoregulatory activity, ADP80-2 can significantly promote the phagocytosis, the production of nitric oxide (NO), and the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage.
33872613	4	38	theme	cytokines	618:626	arg1	phagocytosis					546:557	the phagocytosis	542:557	the phagocytosis	542:557	In terms of immunoregulatory activity, ADP80-2 can significantly promote the phagocytosis, the production of nitric oxide (NO), and the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage.
33872613	4	38	theme	cytokines	618:626	arg1	production					564:573	the production	560:573	the production of nitric oxide (NO)	560:594	In terms of immunoregulatory activity, ADP80-2 can significantly promote the phagocytosis, the production of nitric oxide (NO), and the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage.
33872613	0	39	dep	characteristics	11:25	arg1	properties					69:78	immunoregulatory properties	52:78	Structural characteristics and in vitro and in vivo immunoregulatory properties of a gluco-arabinan from Angelica dahurica.	0:122	Structural characteristics and in vitro and in vivo immunoregulatory properties of a gluco-arabinan from Angelica dahurica.
33872613	3	40	theme	branch	439:444	arg1	residue					460:466	a terminal branch α-L-Araf-(1 → residue	428:466	a terminal branch α-L-Araf-(1 → residue	428:466	The backbone of ADP80-2 comprised →5)-α-L-Araf-(1→, →3, 5)-α-L-Araf-(1→, →6)-α-D-Glcp-(1→, with a terminal branch α-L-Araf-(1 → residue.
33872613	5	41	theme	related	740:746	arg1	chemokines					729:738	the chemokines	725:738	the chemokines related to immunoregulation	725:766	In addition to the cellular immunomodulatory activities, the chemokines related to immunoregulation were significantly increased in the zebrafish model after treated with ADP80-2.
33872613	0	42	theme	immunoregulatory	52:67	arg1	properties					69:78	immunoregulatory properties	52:78	Structural characteristics and in vitro and in vivo immunoregulatory properties of a gluco-arabinan from Angelica dahurica.	0:122	Structural characteristics and in vitro and in vivo immunoregulatory properties of a gluco-arabinan from Angelica dahurica.
33872613	5	43	dep	activities	713:722	arg1	addition					671:678	addition	671:678	addition	671:678	In addition to the cellular immunomodulatory activities, the chemokines related to immunoregulation were significantly increased in the zebrafish model after treated with ADP80-2.
33872613	3	44	theme	α-L-Araf-	446:454	arg1	residue					460:466	a terminal branch α-L-Araf-(1 → residue	428:466	a terminal branch α-L-Araf-(1 → residue	428:466	The backbone of ADP80-2 comprised →5)-α-L-Araf-(1→, →3, 5)-α-L-Araf-(1→, →6)-α-D-Glcp-(1→, with a terminal branch α-L-Araf-(1 → residue.
33872613	7	45	theme	traditional	1112:1122	arg1	medicine					1132:1139	a traditional Chinese medicine	1110:1139	a traditional Chinese medicine	1110:1139	Simultaneously, the discovery of ADP80-2 further revealed the chemical composition of A. dahurica used as a traditional Chinese medicine and spice.
33066269	3	0	theme	bananas	288:294	arg1	relationship					304:315	its relationship	300:315	its relationship with the chemical composition and growing conditions	300:368	However, there is limited research that explores hyperspectral imaging of bananas and its relationship with the chemical composition and growing conditions.
33066269	3	0	theme	bananas	288:294	arg1	imaging					277:283	hyperspectral imaging	263:283	hyperspectral imaging of bananas	263:294	However, there is limited research that explores hyperspectral imaging of bananas and its relationship with the chemical composition and growing conditions.
33066269	8	1	theme	growing	1439:1445	arg1	conditions					1447:1456	the growing conditions	1435:1456	the growing conditions	1435:1456	Since the variation in composition and colour values were related to rainfall and temperature, the spectral information is likely also influenced by the growing conditions.
33066269	1	2	theme	Growing	132:138	arg1	Conditions					140:149	Growing Conditions	132:149	Growing Conditions	132:149	from Different Countries, Their Compositional Traits and Growing Conditions.
33066269	6	3	theme	geographical	960:971	arg1	origins					973:979	different geographical origins	950:979	different geographical origins	950:979	The principal component analysis showed the preliminary separation of bananas from different geographical origins and production systems.
33066269	5	4	theme	starch	763:768	arg1	values					838:843	starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values	763:843	starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values	763:843	The main compositional traits included moisture, starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values were also determined.
33066269	4	5	theme	nm	499:500	arg1	range					502:506	the 400-1000 nm range	486:506	the 400-1000 nm range of the bananas collected from different countries	486:556	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33066269	6	6	from	origins	973:979	arg1	separation					923:932	the preliminary separation	907:932	the preliminary separation of bananas from different geographical origins and production systems	907:1002	The principal component analysis showed the preliminary separation of bananas from different geographical origins and production systems.
33066269	6	7	theme	different	950:958	arg1	origins					973:979	different geographical origins	950:979	different geographical origins	950:979	The principal component analysis showed the preliminary separation of bananas from different geographical origins and production systems.
33066269	3	8	theme	chemical	326:333	arg1	composition					335:345	the chemical composition	322:345	the chemical composition	322:345	However, there is limited research that explores hyperspectral imaging of bananas and its relationship with the chemical composition and growing conditions.
33066269	4	9	theme	400-1000	490:497	arg1	nm					499:500	nm	499:500	nm	499:500	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33066269	6	10	from	systems	996:1002	arg1	separation					923:932	the preliminary separation	907:932	the preliminary separation of bananas from different geographical origins and production systems	907:1002	The principal component analysis showed the preliminary separation of bananas from different geographical origins and production systems.
33066269	5	11	theme	colour	831:836	arg1	values					838:843	starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values	763:843	starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values	763:843	The main compositional traits included moisture, starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values were also determined.
33066269	5	12	theme	main	718:721	arg1	traits					737:742	The main compositional traits	714:742	The main compositional traits	714:742	The main compositional traits included moisture, starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values were also determined.
33066269	4	13	theme	reflectance	458:468	arg1	data					478:481	the visible near-infrared hyperspectral reflectance imaging data	418:481	the visible near-infrared hyperspectral reflectance imaging data	418:481	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33066269	9	14	theme	quality	1533:1539	arg1	improvement					1511:1521	the improvement	1507:1521	the improvement of banana quality and traceability	1507:1556	The results could be useful to the industry for the improvement of banana quality and traceability.
33066269	4	15	theme	compositional	563:575	arg1	temperature					624:634	temperature	624:634	temperature	624:634	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33066269	4	15	theme	compositional	563:575	arg1	traits					577:582	compositional traits	563:582	compositional traits	563:582	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33066269	4	15	theme	compositional	563:575	arg1	altitude					614:621	altitude	614:621	altitude	614:621	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33066269	5	16	theme	compositional	723:735	arg1	traits					737:742	The main compositional traits	714:742	The main compositional traits	714:742	The main compositional traits included moisture, starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values were also determined.
33066269	4	17	theme	hyperspectral	444:456	arg1	data					478:481	the visible near-infrared hyperspectral reflectance imaging data	418:481	the visible near-infrared hyperspectral reflectance imaging data	418:481	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33066269	7	18	theme	moderate	1076:1083	arg1	r					1099:1099	r	1099:1099	r around ±0.50	1099:1112	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	7	18	theme	moderate	1076:1083	arg1	correlations					1085:1096	moderate correlations	1076:1096	moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand	1076:1283	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	5	19	theme	*	827:827	arg1	*					829:829	a*b*	826:829	a*b*	826:829	The main compositional traits included moisture, starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values were also determined.
33066269	5	20	theme	*	825:825	arg1	values					838:843	starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values	763:843	starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values	763:843	The main compositional traits included moisture, starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values were also determined.
33066269	6	21	theme	bananas	937:943	arg1	separation					923:932	the preliminary separation	907:932	the preliminary separation of bananas from different geographical origins and production systems	907:1002	The principal component analysis showed the preliminary separation of bananas from different geographical origins and production systems.
33066269	9	22	theme	traceability	1545:1556	arg1	improvement					1511:1521	the improvement	1507:1521	the improvement of banana quality and traceability	1507:1556	The results could be useful to the industry for the improvement of banana quality and traceability.
33066269	7	23	dep	values	1174:1179	arg1	nm					1252:1253	615-645 nm	1244:1253	615-645 nm	1244:1253	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	7	23	dep	values	1174:1179	arg1	nm					1264:1265	885-985 nm	1256:1265	885-985 nm	1256:1265	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	7	23	dep	values	1174:1179	arg1	*					1183:1183	a*	1182:1183	a*	1182:1183	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	7	23	dep	values	1174:1179	arg1	*					1187:1187	b*	1186:1187	b*	1186:1187	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	7	23	dep	values	1174:1179	arg1	nm					1240:1241	405-525 nm	1232:1241	405-525 nm	1232:1241	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	7	24	dep	r	1099:1099	arg1	<					1117:1117	p < 0.05	1115:1122	p < 0.05	1115:1122	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	6	25	theme	component	881:889	arg1	analysis					891:898	The principal component analysis	867:898	The principal component analysis	867:898	The principal component analysis showed the preliminary separation of bananas from different geographical origins and production systems.
33066269	5	26	theme	protein	786:792	arg1	values					838:843	starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values	763:843	starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values	763:843	The main compositional traits included moisture, starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values were also determined.
33066269	4	27	theme	imaging	470:476	arg1	data					478:481	the visible near-infrared hyperspectral reflectance imaging data	418:481	the visible near-infrared hyperspectral reflectance imaging data	418:481	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33066269	7	28	theme	colour	1167:1172	arg1	values					1174:1179	the carotene, starch content, and colour values	1133:1179	the carotene, starch content, and colour values (a*, b*) on the one hand	1133:1204	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	7	29	theme	b	1186:1186	arg1	*					1183:1183	a*	1182:1183	a*	1182:1183	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	7	29	theme	b	1186:1186	arg1	*					1187:1187	b*	1186:1187	b*	1186:1187	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	3	30	with	imaging	277:283	arg1	conditions					359:368	growing conditions	351:368	growing conditions	351:368	However, there is limited research that explores hyperspectral imaging of bananas and its relationship with the chemical composition and growing conditions.
33066269	3	30	with	imaging	277:283	arg1	composition					335:345	the chemical composition	322:345	the chemical composition	322:345	However, there is limited research that explores hyperspectral imaging of bananas and its relationship with the chemical composition and growing conditions.
33066269	7	31	theme	carotene	1137:1144	arg1	values					1174:1179	the carotene, starch content, and colour values	1133:1179	the carotene, starch content, and colour values (a*, b*) on the one hand	1133:1204	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	6	32	theme	principal	871:879	arg1	analysis					891:898	The principal component analysis	867:898	The principal component analysis	867:898	The principal component analysis showed the preliminary separation of bananas from different geographical origins and production systems.
33066269	5	33	theme	b	828:828	arg1	*					829:829	a*b*	826:829	a*b*	826:829	The main compositional traits included moisture, starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values were also determined.
33066269	5	34	theme	carotene	795:802	arg1	content					804:810	carotene content	795:810	carotene content	795:810	The main compositional traits included moisture, starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values were also determined.
33066269	1	35	dep	Traits	121:126	arg1	Countries					90:98	Different Countries	80:98	Different Countries	80:98	from Different Countries, Their Compositional Traits and Growing Conditions.
33066269	3	36	theme	growing	351:357	arg1	conditions					359:368	growing conditions	351:368	growing conditions	351:368	However, there is limited research that explores hyperspectral imaging of bananas and its relationship with the chemical composition and growing conditions.
33066269	6	37	theme	preliminary	911:921	arg1	separation					923:932	the preliminary separation	907:932	the preliminary separation of bananas from different geographical origins and production systems	907:1002	The principal component analysis showed the preliminary separation of bananas from different geographical origins and production systems.
33066269	5	38	dep	starch	763:768	arg1	*					829:829	a*b*	826:829	a*b*	826:829	The main compositional traits included moisture, starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values were also determined.
33066269	4	39	dep	traits	577:582	arg1	traits					577:582	compositional traits	563:582	compositional traits	563:582	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33066269	4	39	dep	traits	577:582	arg1	rainfall					640:647	rainfall	640:647	rainfall	640:647	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33066269	4	39	dep	traits	577:582	arg1	temperature					624:634	temperature	624:634	temperature	624:634	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33066269	4	39	dep	traits	577:582	arg1	altitude					614:621	altitude	614:621	altitude	614:621	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33066269	4	39	dep	traits	577:582	arg1	the					559:561	the	559:561	the	559:561	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33066269	1	40	theme	Different	80:88	arg1	Countries					90:98	Different Countries	80:98	Different Countries	80:98	from Different Countries, Their Compositional Traits and Growing Conditions.
33066269	8	41	from	variation	1296:1304	arg1	composition					1309:1319	composition	1309:1319	composition	1309:1319	Since the variation in composition and colour values were related to rainfall and temperature, the spectral information is likely also influenced by the growing conditions.
33066269	0	42	theme	Hyperspectral	31:43	arg1	Images					45:50	Hyperspectral Images	31:50	Hyperspectral Images of Bananas	31:61	Study on the Relations between Hyperspectral Images of Bananas (Musa spp.)
33066269	4	43	theme	different	538:546	arg1	countries					548:556	different countries	538:556	different countries	538:556	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33066269	7	44	theme	spectral	1027:1034	arg1	data					1036:1039	The compositional and spectral data	1005:1039	The compositional and spectral data	1005:1039	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	5	45	theme	CIE	820:822	arg1	*					825:825	the CIE L*	816:825	the CIE L*	816:825	The main compositional traits included moisture, starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values were also determined.
33066269	3	46	theme	limited	232:238	arg1	research					240:247	limited research	232:247	limited research that explores hyperspectral imaging of bananas and its relationship with the chemical composition and growing conditions	232:368	However, there is limited research that explores hyperspectral imaging of bananas and its relationship with the chemical composition and growing conditions.
33066269	4	47	theme	near-infrared	430:442	arg1	data					478:481	the visible near-infrared hyperspectral reflectance imaging data	418:481	the visible near-infrared hyperspectral reflectance imaging data	418:481	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33066269	8	48	theme	colour	1325:1330	arg1	values					1332:1337	the variation in composition and colour values	1292:1337	the variation in composition and colour values	1292:1337	Since the variation in composition and colour values were related to rainfall and temperature, the spectral information is likely also influenced by the growing conditions.
33066269	8	48	theme	colour	1325:1330	arg1	related					1344:1350	related	1344:1350	related	1344:1350	Since the variation in composition and colour values were related to rainfall and temperature, the spectral information is likely also influenced by the growing conditions.
33066269	5	49	theme	L	824:824	arg1	*					825:825	the CIE L*	816:825	the CIE L*	816:825	The main compositional traits included moisture, starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values were also determined.
33066269	5	50	theme	fibre	779:783	arg1	values					838:843	starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values	763:843	starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values	763:843	The main compositional traits included moisture, starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values were also determined.
33066269	7	51	theme	content	1154:1160	arg1	values					1174:1179	the carotene, starch content, and colour values	1133:1179	the carotene, starch content, and colour values (a*, b*) on the one hand	1133:1204	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	7	52	theme	compositional	1009:1021	arg1	data					1036:1039	The compositional and spectral data	1005:1039	The compositional and spectral data	1005:1039	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	4	53	theme	visible	422:428	arg1	data					478:481	the visible near-infrared hyperspectral reflectance imaging data	418:481	the visible near-infrared hyperspectral reflectance imaging data	418:481	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33066269	0	54	from	Study	0:4	arg1	Relations					13:21	the Relations	9:21	the Relations between Hyperspectral Images of Bananas	9:61	Study on the Relations between Hyperspectral Images of Bananas (Musa spp.)
33066269	4	55	theme	bananas	515:521	arg1	range					502:506	the 400-1000 nm range	486:506	the 400-1000 nm range of the bananas collected from different countries	486:556	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33066269	7	56	theme	starch	1147:1152	arg1	content					1154:1160	starch content	1147:1160	starch content	1147:1160	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	2	57	theme	popular	181:187	arg1	fruits					189:194	the most popular fruits	172:194	the most popular fruits around the world	172:211	Bananas are some of the most popular fruits around the world.
33066269	9	58	theme	banana	1526:1531	arg1	quality					1533:1539	banana quality	1526:1539	banana quality	1526:1539	The results could be useful to the industry for the improvement of banana quality and traceability.
33066269	0	59	dep	Study	0:4	arg1	spp					69:71	spp	69:71	spp	69:71	Study on the Relations between Hyperspectral Images of Bananas (Musa spp.)
33066269	8	60	theme	variation	1296:1304	arg1	values					1332:1337	the variation in composition and colour values	1292:1337	the variation in composition and colour values	1292:1337	Since the variation in composition and colour values were related to rainfall and temperature, the spectral information is likely also influenced by the growing conditions.
33066269	8	60	theme	variation	1296:1304	arg1	related					1344:1350	related	1344:1350	related	1344:1350	Since the variation in composition and colour values were related to rainfall and temperature, the spectral information is likely also influenced by the growing conditions.
33066269	7	61	theme	p	1115:1115	arg1	<					1117:1117	p < 0.05	1115:1122	p < 0.05	1115:1122	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	8	62	from	colour	1325:1330	arg1	composition					1309:1319	composition	1309:1319	composition	1309:1319	Since the variation in composition and colour values were related to rainfall and temperature, the spectral information is likely also influenced by the growing conditions.
33066269	7	63	theme	other	1274:1278	arg1	hand					1280:1283	the other hand	1270:1283	the other hand	1270:1283	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	4	64	theme	local	588:592	arg1	conditions					602:611	local growing conditions	588:611	local growing conditions	588:611	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33066269	3	65	theme	hyperspectral	263:275	arg1	imaging					277:283	hyperspectral imaging	263:283	hyperspectral imaging of bananas	263:294	However, there is limited research that explores hyperspectral imaging of bananas and its relationship with the chemical composition and growing conditions.
33066269	7	66	from	values	1174:1179	arg1	hand					1201:1204	the one hand	1193:1204	the one hand	1193:1204	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	7	66	from	values	1174:1179	arg1	hand					1280:1283	the other hand	1270:1283	the other hand	1270:1283	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	5	67	theme	content	804:810	arg1	values					838:843	starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values	763:843	starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values	763:843	The main compositional traits included moisture, starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values were also determined.
33066269	1	68	theme	Compositional	107:119	arg1	Traits					121:126	Their Compositional Traits	101:126	Their Compositional Traits	101:126	from Different Countries, Their Compositional Traits and Growing Conditions.
33066269	7	69	theme	wavelength	1214:1223	arg1	ranges					1225:1230	the wavelength ranges	1210:1230	the wavelength ranges	1210:1230	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	6	70	theme	production	985:994	arg1	systems					996:1002	production systems	985:1002	production systems	985:1002	The principal component analysis showed the preliminary separation of bananas from different geographical origins and production systems.
33066269	8	71	theme	spectral	1385:1392	arg1	information					1394:1404	the spectral information	1381:1404	the spectral information	1381:1404	Since the variation in composition and colour values were related to rainfall and temperature, the spectral information is likely also influenced by the growing conditions.
33066269	7	72	from	ranges	1225:1230	arg1	hand					1201:1204	the one hand	1193:1204	the one hand	1193:1204	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	7	72	from	ranges	1225:1230	arg1	hand					1280:1283	the other hand	1270:1283	the other hand	1270:1283	The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405-525 nm, 615-645 nm, 885-985 nm on the other hand.
33066269	0	73	theme	Bananas	55:61	arg1	Images					45:50	Hyperspectral Images	31:50	Hyperspectral Images of Bananas	31:61	Study on the Relations between Hyperspectral Images of Bananas (Musa spp.)
33066269	3	74	with	relationship	304:315	arg1	conditions					359:368	growing conditions	351:368	growing conditions	351:368	However, there is limited research that explores hyperspectral imaging of bananas and its relationship with the chemical composition and growing conditions.
33066269	3	74	with	relationship	304:315	arg1	composition					335:345	the chemical composition	322:345	the chemical composition	322:345	However, there is limited research that explores hyperspectral imaging of bananas and its relationship with the chemical composition and growing conditions.
33066269	4	75	theme	production	654:663	arg1	organic/conventional					677:696	organic/conventional	677:696	organic/conventional	677:696	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33066269	4	75	theme	production	654:663	arg1	management					665:674	production management	654:674	production management (organic/conventional)	654:697	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33066269	4	76	theme	growing	594:600	arg1	conditions					602:611	local growing conditions	588:611	local growing conditions	588:611	In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400-1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored.
33212884	1	0	theme	solvent	233:239	arg1	method					253:258	the solvent evaporation method	229:258	the solvent evaporation method	229:258	Pectin and chitosan films containing glycerol (Gly) at 5, 10, 15, 20, 30, and 40 wt % were prepared in an aqueous HCl solution (0.10 M) by the solvent evaporation method.
33212884	12	1	theme	critical	1498:1505	arg1	viewpoint					1507:1515	a critical viewpoint	1496:1515	a critical viewpoint concerning chitosan-based films with antimicrobial activities	1496:1577	This paper presents a critical viewpoint concerning chitosan-based films with antimicrobial activities.
33212884	8	2	theme	coat	1150:1153	arg1	packaging					1171:1179	coat commercial food packaging	1150:1179	coat commercial food packaging	1150:1179	Therefore, we propose the use of UF40 to coat commercial food packaging.
33212884	1	3	theme	evaporation	241:251	arg1	method					253:258	the solvent evaporation method	229:258	the solvent evaporation method	229:258	Pectin and chitosan films containing glycerol (Gly) at 5, 10, 15, 20, 30, and 40 wt % were prepared in an aqueous HCl solution (0.10 M) by the solvent evaporation method.
33212884	7	4	theme	remaining	865:873	arg1	HCl					875:877	The remaining HCl	861:877	The remaining HCl in the UF40	861:889	The remaining HCl in the UF40 can be a disadvantage for food packaging applications; the UF40 (&empty; = 8.5 mm; 55 μm thickness) releases H3O+/HCl, reducing the pH to approximately 3.0 when kept in 200 mL distilled water for approximately 30 min.
33212884	7	4	theme	remaining	865:873	arg1	disadvantage					900:911	a disadvantage	898:911	a disadvantage for food packaging applications	898:943	The remaining HCl in the UF40 can be a disadvantage for food packaging applications; the UF40 (&empty; = 8.5 mm; 55 μm thickness) releases H3O+/HCl, reducing the pH to approximately 3.0 when kept in 200 mL distilled water for approximately 30 min.
33212884	12	5	theme	chitosan-based	1528:1541	arg1	films					1543:1547	chitosan-based films	1528:1547	chitosan-based films with antimicrobial activities	1528:1577	This paper presents a critical viewpoint concerning chitosan-based films with antimicrobial activities.
33212884	3	6	theme	elongation	419:428	arg1	break					433:437	low elongation at break	415:437	low elongation at break (ε, <5.0%)	415:448	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	11	7	theme	oxidation	1434:1442	arg1	contamination					1461:1473	no oxidation or microorganism contamination	1431:1473	contamination	1461:1473	The UF40 maintained tomatoes' structural integrity for 18 days at room temperature with no oxidation or microorganism contamination.
33212884	7	8	theme	packaging	922:930	arg1	applications					932:943	food packaging applications	917:943	food packaging applications	917:943	The remaining HCl in the UF40 can be a disadvantage for food packaging applications; the UF40 (&empty; = 8.5 mm; 55 μm thickness) releases H3O+/HCl, reducing the pH to approximately 3.0 when kept in 200 mL distilled water for approximately 30 min.
33212884	1	9	contain	containing	116:125	arg2	Gly					137:139	Gly	137:139	Gly	137:139	Pectin and chitosan films containing glycerol (Gly) at 5, 10, 15, 20, 30, and 40 wt % were prepared in an aqueous HCl solution (0.10 M) by the solvent evaporation method.
33212884	1	9	contain	containing	116:125	arg2	glycerol					127:134	glycerol	127:134	glycerol (Gly)	127:140	Pectin and chitosan films containing glycerol (Gly) at 5, 10, 15, 20, 30, and 40 wt % were prepared in an aqueous HCl solution (0.10 M) by the solvent evaporation method.
33212884	1	9	contain	containing	116:125	arg1	films					110:114	Pectin and chitosan films	90:114	films	110:114	Pectin and chitosan films containing glycerol (Gly) at 5, 10, 15, 20, 30, and 40 wt % were prepared in an aqueous HCl solution (0.10 M) by the solvent evaporation method.
33212884	7	10	dep	&empty	956:961	arg1	thickness					980:988	55 μm thickness	974:988	&empty; = 8.5 mm; 55 μm thickness	956:988	The remaining HCl in the UF40 can be a disadvantage for food packaging applications; the UF40 (&empty; = 8.5 mm; 55 μm thickness) releases H3O+/HCl, reducing the pH to approximately 3.0 when kept in 200 mL distilled water for approximately 30 min.
33212884	7	10	dep	&empty	956:961	arg1	mm					970:971	= 8.5 mm	964:971	&empty; = 8.5 mm; 55 μm thickness	956:988	The remaining HCl in the UF40 can be a disadvantage for food packaging applications; the UF40 (&empty; = 8.5 mm; 55 μm thickness) releases H3O+/HCl, reducing the pH to approximately 3.0 when kept in 200 mL distilled water for approximately 30 min.
33212884	11	11	theme	room	1409:1412	arg1	temperature					1414:1424	room temperature	1409:1424	room temperature with no oxidation or microorganism contamination	1409:1473	The UF40 maintained tomatoes' structural integrity for 18 days at room temperature with no oxidation or microorganism contamination.
33212884	2	12	theme	%	350:350	arg1	break					332:336	break	332:336	break (ε, %) of 19%	332:350	The unwashed film (UF) containing 40 wt % Gly (UF40) had elongation at break (ε, %) of 19%.
33212884	2	13	dep	break	332:336	arg1	%					342:342	%	342:342	%	342:342	The unwashed film (UF) containing 40 wt % Gly (UF40) had elongation at break (ε, %) of 19%.
33212884	2	13	dep	break	332:336	arg1	ε					339:339	ε	339:339	ε	339:339	The unwashed film (UF) containing 40 wt % Gly (UF40) had elongation at break (ε, %) of 19%.
33212884	11	14	theme	microorganism	1447:1459	arg1	contamination					1461:1473	no oxidation or microorganism contamination	1431:1473	contamination	1461:1473	The UF40 maintained tomatoes' structural integrity for 18 days at room temperature with no oxidation or microorganism contamination.
33212884	3	15	theme	food	473:476	arg1	applications					488:499	food packaging applications	473:499	food packaging applications	473:499	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	5	16	theme	low	677:679	arg1	content					686:692	a low acid content	675:692	a low acid content on the UF surfaces	675:711	The XPS analysis suggests that some HCl is lost during the drying process, resulting in a low acid content on the UF surfaces.
33212884	2	17	contain	had	314:316	arg1	UF					280:281	UF	280:281	UF	280:281	The unwashed film (UF) containing 40 wt % Gly (UF40) had elongation at break (ε, %) of 19%.
33212884	2	17	contain	had	314:316	arg2	elongation					318:327	elongation	318:327	elongation	318:327	The unwashed film (UF) containing 40 wt % Gly (UF40) had elongation at break (ε, %) of 19%.
33212884	2	17	contain	had	314:316	arg1	film					274:277	The unwashed film	261:277	The unwashed film (UF) containing 40 wt % Gly (UF40)	261:312	The unwashed film (UF) containing 40 wt % Gly (UF40) had elongation at break (ε, %) of 19%.
33212884	3	18	theme	Washed	353:358	arg1	WFs					367:369	WFs	367:369	WFs	367:369	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	3	18	theme	Washed	353:358	arg1	films					360:364	Washed films	353:364	Washed films (WFs)	353:370	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	9	19	theme	water	1215:1219	arg1	vapor					1221:1225	water vapor	1215:1225	water vapor	1215:1225	The UF40 has low permeability to water vapor and oxygen and works as a barrier against ultraviolet light.
33212884	3	20	from	break	433:437	arg1	at					430:431	low elongation at break	415:437	low elongation at break (ε, <5.0%)	415:448	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	3	20	from	break	433:437	arg1	elongation					419:428	low elongation at break	415:437	low elongation at break (ε, <5.0%)	415:448	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	12	21	theme	antimicrobial	1554:1566	arg1	activities					1568:1577	antimicrobial activities	1554:1577	antimicrobial activities	1554:1577	This paper presents a critical viewpoint concerning chitosan-based films with antimicrobial activities.
33212884	3	22	from	use	466:468	arg1	applications					488:499	food packaging applications	473:499	food packaging applications	473:499	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	6	23	theme	anti-adhesive	810:822	arg1	properties					824:833	antimicrobial and anti-adhesive properties	792:833	antimicrobial and anti-adhesive properties	792:833	The UF40 (at 5.0 mg/mL) exhibits cytocompatibility toward mammalian cells and antimicrobial and anti-adhesive properties against Escherichia coli.
33212884	6	24	theme	antimicrobial	792:804	arg1	properties					824:833	antimicrobial and anti-adhesive properties	792:833	antimicrobial and anti-adhesive properties	792:833	The UF40 (at 5.0 mg/mL) exhibits cytocompatibility toward mammalian cells and antimicrobial and anti-adhesive properties against Escherichia coli.
33212884	0	25	theme	Pectin/Chitosan/Glycerol	13:36	arg1	Films					38:42	Bactericidal Pectin/Chitosan/Glycerol Films	0:42	Bactericidal Pectin/Chitosan/Glycerol Films for Food Pack Coatings	0:65	Bactericidal Pectin/Chitosan/Glycerol Films for Food Pack Coatings: A Critical Viewpoint.
33212884	3	26	contain	had	372:374	arg2	strength					389:396	high tensile strength	376:396	high tensile strength (σ > 46 MPa)	376:409	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	3	26	contain	had	372:374	arg2	MPa					406:408	σ > 46 MPa	399:408	σ > 46 MPa	399:408	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	3	26	contain	had	372:374	arg1	WFs					367:369	WFs	367:369	WFs	367:369	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	3	26	contain	had	372:374	arg2	break					433:437	low elongation at break	415:437	low elongation at break (ε, <5.0%)	415:448	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	3	26	contain	had	372:374	arg1	films					360:364	Washed films	353:364	Washed films (WFs)	353:370	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	1	27	theme	Pectin	90:95	arg1	films					110:114	Pectin and chitosan films	90:114	films	110:114	Pectin and chitosan films containing glycerol (Gly) at 5, 10, 15, 20, 30, and 40 wt % were prepared in an aqueous HCl solution (0.10 M) by the solvent evaporation method.
33212884	0	28	theme	Bactericidal	0:11	arg1	Films					38:42	Bactericidal Pectin/Chitosan/Glycerol Films	0:42	Bactericidal Pectin/Chitosan/Glycerol Films for Food Pack Coatings	0:65	Bactericidal Pectin/Chitosan/Glycerol Films for Food Pack Coatings: A Critical Viewpoint.
33212884	1	29	theme	aqueous	196:202	arg1	solution					208:215	an aqueous HCl solution	193:215	an aqueous HCl solution (0.10 M)	193:224	Pectin and chitosan films containing glycerol (Gly) at 5, 10, 15, 20, 30, and 40 wt % were prepared in an aqueous HCl solution (0.10 M) by the solvent evaporation method.
33212884	1	29	theme	aqueous	196:202	arg1	M					223:223	0.10 M	218:223	0.10 M	218:223	Pectin and chitosan films containing glycerol (Gly) at 5, 10, 15, 20, 30, and 40 wt % were prepared in an aqueous HCl solution (0.10 M) by the solvent evaporation method.
33212884	0	30	dep	Viewpoint	79:87	arg1	Films					38:42	Bactericidal Pectin/Chitosan/Glycerol Films	0:42	Bactericidal Pectin/Chitosan/Glycerol Films for Food Pack Coatings	0:65	Bactericidal Pectin/Chitosan/Glycerol Films for Food Pack Coatings: A Critical Viewpoint.
33212884	1	31	theme	HCl	204:206	arg1	solution					208:215	an aqueous HCl solution	193:215	an aqueous HCl solution (0.10 M)	193:224	Pectin and chitosan films containing glycerol (Gly) at 5, 10, 15, 20, 30, and 40 wt % were prepared in an aqueous HCl solution (0.10 M) by the solvent evaporation method.
33212884	1	31	theme	HCl	204:206	arg1	M					223:223	0.10 M	218:223	0.10 M	218:223	Pectin and chitosan films containing glycerol (Gly) at 5, 10, 15, 20, 30, and 40 wt % were prepared in an aqueous HCl solution (0.10 M) by the solvent evaporation method.
33212884	1	32	theme	chitosan	101:108	arg1	films					110:114	Pectin and chitosan films	90:114	films	110:114	Pectin and chitosan films containing glycerol (Gly) at 5, 10, 15, 20, 30, and 40 wt % were prepared in an aqueous HCl solution (0.10 M) by the solvent evaporation method.
33212884	7	33	theme	200	1060:1062	arg1	mL					1064:1065	mL	1064:1065	mL	1064:1065	The remaining HCl in the UF40 can be a disadvantage for food packaging applications; the UF40 (&empty; = 8.5 mm; 55 μm thickness) releases H3O+/HCl, reducing the pH to approximately 3.0 when kept in 200 mL distilled water for approximately 30 min.
33212884	6	34	theme	mammalian	772:780	arg1	cells					782:786	mammalian cells	772:786	mammalian cells	772:786	The UF40 (at 5.0 mg/mL) exhibits cytocompatibility toward mammalian cells and antimicrobial and anti-adhesive properties against Escherichia coli.
33212884	3	35	theme	tensile	381:387	arg1	MPa					406:408	σ > 46 MPa	399:408	σ > 46 MPa	399:408	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	3	35	theme	tensile	381:387	arg1	strength					389:396	high tensile strength	376:396	high tensile strength (σ > 46 MPa)	376:409	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	3	36	theme	packaging	478:486	arg1	applications					488:499	food packaging applications	473:499	food packaging applications	473:499	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	5	37	theme	UF	701:702	arg1	surfaces					704:711	the UF surfaces	697:711	the UF surfaces	697:711	The XPS analysis suggests that some HCl is lost during the drying process, resulting in a low acid content on the UF surfaces.
33212884	3	38	theme	at	430:431	arg1	break					433:437	low elongation at break	415:437	low elongation at break (ε, <5.0%)	415:448	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	5	39	theme	drying	646:651	arg1	process					653:659	the drying process	642:659	the drying process	642:659	The XPS analysis suggests that some HCl is lost during the drying process, resulting in a low acid content on the UF surfaces.
33212884	5	40	theme	XPS	591:593	arg1	analysis					595:602	The XPS analysis	587:602	The XPS analysis	587:602	The XPS analysis suggests that some HCl is lost during the drying process, resulting in a low acid content on the UF surfaces.
33212884	0	41	theme	Pack	53:56	arg1	Coatings					58:65	Food Pack Coatings	48:65	Food Pack Coatings	48:65	Bactericidal Pectin/Chitosan/Glycerol Films for Food Pack Coatings: A Critical Viewpoint.
33212884	7	42	dep	UF40	950:953	arg1	&empty					956:961	&empty	956:961	&empty; = 8.5 mm; 55 μm thickness	956:988	The remaining HCl in the UF40 can be a disadvantage for food packaging applications; the UF40 (&empty; = 8.5 mm; 55 μm thickness) releases H3O+/HCl, reducing the pH to approximately 3.0 when kept in 200 mL distilled water for approximately 30 min.
33212884	3	43	theme	σ	399:399	arg1	MPa					406:408	σ > 46 MPa	399:408	σ > 46 MPa	399:408	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	3	43	theme	σ	399:399	arg1	strength					389:396	high tensile strength	376:396	high tensile strength (σ > 46 MPa)	376:409	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	2	44	theme	wt	298:299	arg1	%					301:301	40 wt %	295:301	40 wt % Gly (UF40)	295:312	The unwashed film (UF) containing 40 wt % Gly (UF40) had elongation at break (ε, %) of 19%.
33212884	0	45	theme	Food	48:51	arg1	Coatings					58:65	Food Pack Coatings	48:65	Food Pack Coatings	48:65	Bactericidal Pectin/Chitosan/Glycerol Films for Food Pack Coatings: A Critical Viewpoint.
33212884	7	46	theme	distilled	1067:1075	arg1	water					1077:1081	200 mL distilled water	1060:1081	200 mL distilled water	1060:1081	The remaining HCl in the UF40 can be a disadvantage for food packaging applications; the UF40 (&empty; = 8.5 mm; 55 μm thickness) releases H3O+/HCl, reducing the pH to approximately 3.0 when kept in 200 mL distilled water for approximately 30 min.
33212884	3	47	theme	low	415:417	arg1	break					433:437	low elongation at break	415:437	low elongation at break (ε, <5.0%)	415:448	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	3	48	theme	>	401:401	arg1	MPa					406:408	σ > 46 MPa	399:408	σ > 46 MPa	399:408	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	3	48	theme	>	401:401	arg1	strength					389:396	high tensile strength	376:396	high tensile strength (σ > 46 MPa)	376:409	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	11	49	theme	structural	1373:1382	arg1	integrity					1384:1392	tomatoes' structural integrity	1363:1392	tomatoes' structural integrity for 18 days	1363:1404	The UF40 maintained tomatoes' structural integrity for 18 days at room temperature with no oxidation or microorganism contamination.
33212884	7	50	theme	food	917:920	arg1	applications					932:943	food packaging applications	917:943	food packaging applications	917:943	The remaining HCl in the UF40 can be a disadvantage for food packaging applications; the UF40 (&empty; = 8.5 mm; 55 μm thickness) releases H3O+/HCl, reducing the pH to approximately 3.0 when kept in 200 mL distilled water for approximately 30 min.
33212884	9	51	contain	has	1191:1193	arg1	UF40					1186:1189	The UF40	1182:1189	The UF40	1182:1189	The UF40 has low permeability to water vapor and oxygen and works as a barrier against ultraviolet light.
33212884	9	51	contain	has	1191:1193	arg2	permeability					1199:1210	low permeability	1195:1210	low permeability to water vapor and oxygen	1195:1236	The UF40 has low permeability to water vapor and oxygen and works as a barrier against ultraviolet light.
33212884	2	52	contain	containing	284:293	arg2	%					301:301	40 wt %	295:301	40 wt % Gly (UF40)	295:312	The unwashed film (UF) containing 40 wt % Gly (UF40) had elongation at break (ε, %) of 19%.
33212884	2	52	contain	containing	284:293	arg1	UF					280:281	UF	280:281	UF	280:281	The unwashed film (UF) containing 40 wt % Gly (UF40) had elongation at break (ε, %) of 19%.
33212884	2	52	contain	containing	284:293	arg1	film					274:277	The unwashed film	261:277	The unwashed film (UF) containing 40 wt % Gly (UF40)	261:312	The unwashed film (UF) containing 40 wt % Gly (UF40) had elongation at break (ε, %) of 19%.
33212884	11	53	with	temperature	1414:1424	arg1	contamination					1461:1473	no oxidation or microorganism contamination	1431:1473	contamination	1461:1473	The UF40 maintained tomatoes' structural integrity for 18 days at room temperature with no oxidation or microorganism contamination.
33212884	8	54	theme	food	1166:1169	arg1	packaging					1171:1179	coat commercial food packaging	1150:1179	coat commercial food packaging	1150:1179	Therefore, we propose the use of UF40 to coat commercial food packaging.
33212884	8	55	theme	commercial	1155:1164	arg1	packaging					1171:1179	coat commercial food packaging	1150:1179	coat commercial food packaging	1150:1179	Therefore, we propose the use of UF40 to coat commercial food packaging.
33212884	9	56	theme	low	1195:1197	arg1	permeability					1199:1210	low permeability	1195:1210	low permeability to water vapor and oxygen	1195:1236	The UF40 has low permeability to water vapor and oxygen and works as a barrier against ultraviolet light.
33212884	2	57	dep	%	301:301	arg1	Gly					303:305	Gly (UF40)	303:312	40 wt % Gly (UF40)	295:312	The unwashed film (UF) containing 40 wt % Gly (UF40) had elongation at break (ε, %) of 19%.
33212884	7	58	theme	μm	977:978	arg1	thickness					980:988	55 μm thickness	974:988	&empty; = 8.5 mm; 55 μm thickness	956:988	The remaining HCl in the UF40 can be a disadvantage for food packaging applications; the UF40 (&empty; = 8.5 mm; 55 μm thickness) releases H3O+/HCl, reducing the pH to approximately 3.0 when kept in 200 mL distilled water for approximately 30 min.
33212884	10	59	dep	colorless	1305:1313	arg1	UF40					1292:1295	The UF40	1288:1295	The UF40	1288:1295	The UF40 is also colorless and completely transparent.
33212884	10	59	dep	colorless	1305:1313	arg1	colorless					1305:1313	colorless	1305:1313	colorless	1305:1313	The UF40 is also colorless and completely transparent.
33212884	10	59	dep	colorless	1305:1313	arg1	transparent					1330:1340	transparent	1330:1340	transparent	1330:1340	The UF40 is also colorless and completely transparent.
33212884	3	60	theme	high	376:379	arg1	MPa					406:408	σ > 46 MPa	399:408	σ > 46 MPa	399:408	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	3	60	theme	high	376:379	arg1	strength					389:396	high tensile strength	376:396	high tensile strength (σ > 46 MPa)	376:409	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	12	61	with	films	1543:1547	arg1	activities					1568:1577	antimicrobial activities	1554:1577	antimicrobial activities	1554:1577	This paper presents a critical viewpoint concerning chitosan-based films with antimicrobial activities.
33212884	7	62	theme	mL	1064:1065	arg1	water					1077:1081	200 mL distilled water	1060:1081	200 mL distilled water	1060:1081	The remaining HCl in the UF40 can be a disadvantage for food packaging applications; the UF40 (&empty; = 8.5 mm; 55 μm thickness) releases H3O+/HCl, reducing the pH to approximately 3.0 when kept in 200 mL distilled water for approximately 30 min.
33212884	5	63	theme	acid	681:684	arg1	content					686:692	a low acid content	675:692	a low acid content on the UF surfaces	675:711	The XPS analysis suggests that some HCl is lost during the drying process, resulting in a low acid content on the UF surfaces.
33212884	4	64	theme	self-assembling	516:530	arg1	polymers					506:513	The polymers	502:513	The polymers' self-assembling	502:530	The polymers' self-assembling occurred during the washing, increasing the stiffness.
33212884	9	65	theme	ultraviolet	1269:1279	arg1	light					1281:1285	ultraviolet light	1269:1285	ultraviolet light	1269:1285	The UF40 has low permeability to water vapor and oxygen and works as a barrier against ultraviolet light.
33212884	7	66	theme	8.5	966:968	arg1	mm					970:971	= 8.5 mm	964:971	&empty; = 8.5 mm; 55 μm thickness	956:988	The remaining HCl in the UF40 can be a disadvantage for food packaging applications; the UF40 (&empty; = 8.5 mm; 55 μm thickness) releases H3O+/HCl, reducing the pH to approximately 3.0 when kept in 200 mL distilled water for approximately 30 min.
33212884	0	67	theme	Critical	70:77	arg1	Viewpoint					79:87	A Critical Viewpoint	68:87	A Critical Viewpoint	68:87	Bactericidal Pectin/Chitosan/Glycerol Films for Food Pack Coatings: A Critical Viewpoint.
33212884	8	68	theme	UF40	1142:1145	arg1	use					1135:1137	the use	1131:1137	the use of UF40 to coat commercial food packaging	1131:1179	Therefore, we propose the use of UF40 to coat commercial food packaging.
33212884	6	69	from	mg/mL	731:735	arg1	UF40					718:721	The UF40	714:721	The UF40 (at 5.0 mg/mL)	714:736	The UF40 (at 5.0 mg/mL) exhibits cytocompatibility toward mammalian cells and antimicrobial and anti-adhesive properties against Escherichia coli.
33212884	7	70	theme	=	964:964	arg1	mm					970:971	= 8.5 mm	964:971	&empty; = 8.5 mm; 55 μm thickness	956:988	The remaining HCl in the UF40 can be a disadvantage for food packaging applications; the UF40 (&empty; = 8.5 mm; 55 μm thickness) releases H3O+/HCl, reducing the pH to approximately 3.0 when kept in 200 mL distilled water for approximately 30 min.
33212884	5	71	from	content	686:692	arg1	surfaces					704:711	the UF surfaces	697:711	the UF surfaces	697:711	The XPS analysis suggests that some HCl is lost during the drying process, resulting in a low acid content on the UF surfaces.
33212884	1	72	theme	wt	171:172	arg1	%					174:174	5, 10, 15, 20, 30, and 40 wt %	145:174	5, 10, 15, 20, 30, and 40 wt %	145:174	Pectin and chitosan films containing glycerol (Gly) at 5, 10, 15, 20, 30, and 40 wt % were prepared in an aqueous HCl solution (0.10 M) by the solvent evaporation method.
33212884	7	73	from	HCl	875:877	arg1	UF40					886:889	the UF40	882:889	the UF40	882:889	The remaining HCl in the UF40 can be a disadvantage for food packaging applications; the UF40 (&empty; = 8.5 mm; 55 μm thickness) releases H3O+/HCl, reducing the pH to approximately 3.0 when kept in 200 mL distilled water for approximately 30 min.
33212884	2	74	theme	unwashed	265:272	arg1	UF					280:281	UF	280:281	UF	280:281	The unwashed film (UF) containing 40 wt % Gly (UF40) had elongation at break (ε, %) of 19%.
33212884	2	74	theme	unwashed	265:272	arg1	film					274:277	The unwashed film	261:277	The unwashed film (UF) containing 40 wt % Gly (UF40)	261:312	The unwashed film (UF) containing 40 wt % Gly (UF40) had elongation at break (ε, %) of 19%.
33212884	3	75	dep	break	433:437	arg1	ε					440:440	ε	440:440	ε	440:440	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
33212884	3	75	dep	break	433:437	arg1	%					447:447	<5.0%	443:447	<5.0%	443:447	Washed films (WFs) had high tensile strength (σ > 46 MPa) and low elongation at break (ε, <5.0%), enabling their use in food packaging applications.
34651208	4	0	theme	CS	1039:1040	arg1	4-O-endosulfatase					1042:1058	a CS 4-O-endosulfatase	1037:1058	a CS 4-O-endosulfatase	1037:1058	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	4	1	theme	biological	902:911	arg1	media					922:926	biological reaction media	902:926	biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC	902:1126	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	4	2	theme	various	943:949	arg1	desulfation					1002:1012	enzymatic desulfation	992:1012	enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase	992:1058	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	4	2	theme	various	943:949	arg1	transformations					961:975	various enzymatic transformations	943:975	various enzymatic transformations	943:975	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	2	3	theme	mass	391:394	arg1	MS					410:411	MS	410:411	MS	410:411	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	2	3	theme	mass	391:394	arg1	spectrometry					396:407	its on-line coupling to mass spectrometry	367:407	its on-line coupling to mass spectrometry (MS)	367:412	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	4	4	theme	CS	1089:1090	arg1	endocan					1092:1098	the CS endocan	1085:1098	the CS endocan	1085:1098	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	2	5	theme	disaccharides	507:519	arg1	differentiation					433:447	efficient differentiation	423:447	efficient differentiation	423:447	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	2	5	theme	disaccharides	507:519	arg1	detection					463:471	sensitive detection	453:471	sensitive detection	453:471	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	4	6	theme	chondroitinase	1103:1116	arg1	ABC					1124:1126	chondroitinase lyase ABC	1103:1126	chondroitinase lyase ABC	1103:1126	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	4	7	theme	media	922:926	arg1	composition					887:897	the disaccharide composition	870:897	the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC	870:1126	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	2	8	theme	interaction	311:321	arg1	method					356:361	a hydrophilic interaction liquid chromatography separation method	297:361	a hydrophilic interaction liquid chromatography separation method	297:361	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	3	9	from	influence	673:681	arg1	separation					706:715	the chromatographic separation	686:715	the chromatographic separation	686:715	The composition of the mobile phase in terms of pH and concentration showed great influence on the chromatographic separation and was varied to allow the distinction of each CS without signal overlap for a total analysis time of 25 min.
34651208	4	10	theme	reaction	913:920	arg1	media					922:926	biological reaction media	902:926	biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC	902:1126	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	2	11	theme	hydrophilic	299:309	arg1	method					356:361	a hydrophilic interaction liquid chromatography separation method	297:361	a hydrophilic interaction liquid chromatography separation method	297:361	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	3	12	theme	CS	765:766	arg1	distinction					745:755	the distinction	741:755	the distinction of each CS without signal overlap for a total analysis time of 25 min	741:825	The composition of the mobile phase in terms of pH and concentration showed great influence on the chromatographic separation and was varied to allow the distinction of each CS without signal overlap for a total analysis time of 25 min.
34651208	2	13	theme	sensitive	453:461	arg1	detection					463:471	sensitive detection	453:471	sensitive detection	453:471	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	2	14	theme	efficient	423:431	arg1	differentiation					433:447	efficient differentiation	423:447	efficient differentiation	423:447	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	4	15	theme	enzymatic	992:1000	arg1	desulfation					1002:1012	enzymatic desulfation	992:1012	enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase	992:1058	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	2	16	theme	CS	504:505	arg1	disaccharides					507:519	trisulfated CS disaccharides	492:519	trisulfated CS disaccharides	492:519	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	1	17	theme	Chondroitin	85:95	arg1	CS					106:107	CS	106:107	CS	106:107	Chondroitin sulfate (CS) glycosaminoglycans are biologically active sulfated polysaccharides that pose an analytical challenge for their structural analysis and functional evaluation.
34651208	1	17	theme	Chondroitin	85:95	arg1	sulfate					97:103	Chondroitin sulfate	85:103	Chondroitin sulfate (CS) glycosaminoglycans	85:127	Chondroitin sulfate (CS) glycosaminoglycans are biologically active sulfated polysaccharides that pose an analytical challenge for their structural analysis and functional evaluation.
34651208	1	18	theme	analytical	191:200	arg1	challenge					202:210	an analytical challenge	188:210	an analytical challenge for their structural analysis and functional evaluation	188:266	Chondroitin sulfate (CS) glycosaminoglycans are biologically active sulfated polysaccharides that pose an analytical challenge for their structural analysis and functional evaluation.
34651208	2	19	theme	di-	483:485	arg1	differentiation					433:447	efficient differentiation	423:447	efficient differentiation	423:447	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	2	19	theme	di-	483:485	arg1	detection					463:471	sensitive detection	453:471	sensitive detection	453:471	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	4	20	theme	disaccharides	1020:1032	arg1	desulfation					1002:1012	enzymatic desulfation	992:1012	enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase	992:1058	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	4	21	theme	CS	1017:1018	arg1	disaccharides					1020:1032	CS disaccharides	1017:1032	CS disaccharides	1017:1032	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	1	22	theme	sulfate	97:103	arg1	polysaccharides					162:176	biologically active sulfated polysaccharides	133:176	biologically active sulfated polysaccharides that pose an analytical challenge for their structural analysis and functional evaluation	133:266	Chondroitin sulfate (CS) glycosaminoglycans are biologically active sulfated polysaccharides that pose an analytical challenge for their structural analysis and functional evaluation.
34651208	1	22	theme	sulfate	97:103	arg1	glycosaminoglycans					110:127	Chondroitin sulfate (CS) glycosaminoglycans	85:127	Chondroitin sulfate (CS) glycosaminoglycans	85:127	Chondroitin sulfate (CS) glycosaminoglycans are biologically active sulfated polysaccharides that pose an analytical challenge for their structural analysis and functional evaluation.
34651208	4	23	theme	disaccharide	874:885	arg1	composition					887:897	the disaccharide composition	870:897	the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC	870:1126	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	2	24	theme	separation	345:354	arg1	method					356:361	a hydrophilic interaction liquid chromatography separation method	297:361	a hydrophilic interaction liquid chromatography separation method	297:361	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	3	25	from	phase	621:625	arg1	terms					630:634	terms	630:634	terms of pH and concentration	630:658	The composition of the mobile phase in terms of pH and concentration showed great influence on the chromatographic separation and was varied to allow the distinction of each CS without signal overlap for a total analysis time of 25 min.
34651208	2	26	theme	mono-	476:480	arg1	differentiation					433:447	efficient differentiation	423:447	efficient differentiation	423:447	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	2	26	theme	mono-	476:480	arg1	detection					463:471	sensitive detection	453:471	sensitive detection	453:471	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	2	27	theme	chromatography	330:343	arg1	method					356:361	a hydrophilic interaction liquid chromatography separation method	297:361	a hydrophilic interaction liquid chromatography separation method	297:361	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	0	28	theme	sulfated	18:25	arg1	isomers					27:33	sulfated isomers	18:33	sulfated isomers of chondroitin sulfate	18:56	Discrimination of sulfated isomers of chondroitin sulfate disaccharides by HILIC-MS.
34651208	4	29	theme	CS	980:981	arg1	depolymerization					1065:1080	depolymerization	1065:1080	depolymerization of the CS endocan by chondroitinase lyase ABC	1065:1126	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	4	29	theme	CS	980:981	arg1	desulfation					1002:1012	enzymatic desulfation	992:1012	enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase	992:1058	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	4	29	theme	CS	980:981	arg1	transformations					961:975	various enzymatic transformations	943:975	various enzymatic transformations	943:975	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	2	30	theme	liquid	323:328	arg1	method					356:361	a hydrophilic interaction liquid chromatography separation method	297:361	a hydrophilic interaction liquid chromatography separation method	297:361	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	3	31	from	terms	630:634	arg1	composition					595:605	The composition	591:605	The composition of the mobile phase in terms of pH and concentration	591:658	The composition of the mobile phase in terms of pH and concentration showed great influence on the chromatographic separation and was varied to allow the distinction of each CS without signal overlap for a total analysis time of 25 min.
34651208	1	32	theme	structural	222:231	arg1	analysis					233:240	their structural analysis	216:240	their structural analysis	216:240	Chondroitin sulfate (CS) glycosaminoglycans are biologically active sulfated polysaccharides that pose an analytical challenge for their structural analysis and functional evaluation.
34651208	0	33	theme	chondroitin	38:48	arg1	sulfate					50:56	chondroitin sulfate	38:56	chondroitin sulfate	38:56	Discrimination of sulfated isomers of chondroitin sulfate disaccharides by HILIC-MS.
34651208	2	34	theme	trisulfated	492:502	arg1	disaccharides					507:519	trisulfated CS disaccharides	492:519	trisulfated CS disaccharides	492:519	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	3	35	theme	analysis	803:810	arg1	time					812:815	a total analysis time	795:815	a total analysis time of 25 min	795:825	The composition of the mobile phase in terms of pH and concentration showed great influence on the chromatographic separation and was varied to allow the distinction of each CS without signal overlap for a total analysis time of 25 min.
34651208	2	36	theme	prior	569:573	arg1	derivatization					575:588	prior derivatization	569:588	prior derivatization	569:588	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	3	37	theme	great	667:671	arg1	influence					673:681	great influence	667:681	great influence on the chromatographic separation	667:715	The composition of the mobile phase in terms of pH and concentration showed great influence on the chromatographic separation and was varied to allow the distinction of each CS without signal overlap for a total analysis time of 25 min.
34651208	0	38	theme	isomers	27:33	arg1	Discrimination					0:13	Discrimination	0:13	Discrimination of sulfated isomers of chondroitin sulfate	0:56	Discrimination of sulfated isomers of chondroitin sulfate disaccharides by HILIC-MS.
34651208	3	39	theme	phase	621:625	arg1	composition					595:605	The composition	591:605	The composition of the mobile phase in terms of pH and concentration	591:658	The composition of the mobile phase in terms of pH and concentration showed great influence on the chromatographic separation and was varied to allow the distinction of each CS without signal overlap for a total analysis time of 25 min.
34651208	2	40	theme	coupling	379:386	arg1	MS					410:411	MS	410:411	MS	410:411	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	2	40	theme	coupling	379:386	arg1	spectrometry					396:407	its on-line coupling to mass spectrometry	367:407	its on-line coupling to mass spectrometry (MS)	367:412	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	0	41	theme	sulfate	50:56	arg1	isomers					27:33	sulfated isomers	18:33	sulfated isomers of chondroitin sulfate	18:56	Discrimination of sulfated isomers of chondroitin sulfate disaccharides by HILIC-MS.
34651208	2	42	theme	on-line	371:377	arg1	MS					410:411	MS	410:411	MS	410:411	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	2	42	theme	on-line	371:377	arg1	spectrometry					396:407	its on-line coupling to mass spectrometry	367:407	its on-line coupling to mass spectrometry (MS)	367:412	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	3	43	from	composition	595:605	arg1	terms					630:634	terms	630:634	terms of pH and concentration	630:658	The composition of the mobile phase in terms of pH and concentration showed great influence on the chromatographic separation and was varied to allow the distinction of each CS without signal overlap for a total analysis time of 25 min.
34651208	3	44	theme	total	797:801	arg1	time					812:815	a total analysis time	795:815	a total analysis time of 25 min	795:825	The composition of the mobile phase in terms of pH and concentration showed great influence on the chromatographic separation and was varied to allow the distinction of each CS without signal overlap for a total analysis time of 25 min.
34651208	4	45	theme	enzymatic	951:959	arg1	desulfation					1002:1012	enzymatic desulfation	992:1012	enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase	992:1058	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	4	45	theme	enzymatic	951:959	arg1	transformations					961:975	various enzymatic transformations	943:975	various enzymatic transformations	943:975	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	3	46	theme	concentration	646:658	arg1	terms					630:634	terms	630:634	terms of pH and concentration	630:658	The composition of the mobile phase in terms of pH and concentration showed great influence on the chromatographic separation and was varied to allow the distinction of each CS without signal overlap for a total analysis time of 25 min.
34651208	4	47	theme	lyase	1118:1122	arg1	ABC					1124:1126	chondroitinase lyase ABC	1103:1126	chondroitinase lyase ABC	1103:1126	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	1	48	theme	functional	246:255	arg1	evaluation					257:266	functional evaluation	246:266	functional evaluation	246:266	Chondroitin sulfate (CS) glycosaminoglycans are biologically active sulfated polysaccharides that pose an analytical challenge for their structural analysis and functional evaluation.
34651208	2	49	dep	spectrometry	396:407	arg1	to					388:389	to	388:389	to	388:389	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	3	50	theme	chromatographic	690:704	arg1	separation					706:715	the chromatographic separation	686:715	the chromatographic separation	686:715	The composition of the mobile phase in terms of pH and concentration showed great influence on the chromatographic separation and was varied to allow the distinction of each CS without signal overlap for a total analysis time of 25 min.
34651208	1	51	theme	active	146:151	arg1	polysaccharides					162:176	biologically active sulfated polysaccharides	133:176	biologically active sulfated polysaccharides that pose an analytical challenge for their structural analysis and functional evaluation	133:266	Chondroitin sulfate (CS) glycosaminoglycans are biologically active sulfated polysaccharides that pose an analytical challenge for their structural analysis and functional evaluation.
34651208	1	51	theme	active	146:151	arg1	glycosaminoglycans					110:127	Chondroitin sulfate (CS) glycosaminoglycans	85:127	Chondroitin sulfate (CS) glycosaminoglycans	85:127	Chondroitin sulfate (CS) glycosaminoglycans are biologically active sulfated polysaccharides that pose an analytical challenge for their structural analysis and functional evaluation.
34651208	2	52	theme	positional	531:540	arg1	isomers					542:548	their positional isomers	525:548	their positional isomers	525:548	In this study, we developed a hydrophilic interaction liquid chromatography separation method and its on-line coupling to mass spectrometry (MS) allowing efficient differentiation and sensitive detection of mono-, di-, and trisulfated CS disaccharides and their positional isomers, without requiring prior derivatization.
34651208	3	53	theme	pH	639:640	arg1	terms					630:634	terms	630:634	terms of pH and concentration	630:658	The composition of the mobile phase in terms of pH and concentration showed great influence on the chromatographic separation and was varied to allow the distinction of each CS without signal overlap for a total analysis time of 25 min.
34651208	1	54	theme	sulfated	153:160	arg1	polysaccharides					162:176	biologically active sulfated polysaccharides	133:176	biologically active sulfated polysaccharides that pose an analytical challenge for their structural analysis and functional evaluation	133:266	Chondroitin sulfate (CS) glycosaminoglycans are biologically active sulfated polysaccharides that pose an analytical challenge for their structural analysis and functional evaluation.
34651208	1	54	theme	sulfated	153:160	arg1	glycosaminoglycans					110:127	Chondroitin sulfate (CS) glycosaminoglycans	85:127	Chondroitin sulfate (CS) glycosaminoglycans	85:127	Chondroitin sulfate (CS) glycosaminoglycans are biologically active sulfated polysaccharides that pose an analytical challenge for their structural analysis and functional evaluation.
34651208	3	55	theme	mobile	614:619	arg1	phase					621:625	the mobile phase	610:625	the mobile phase in terms of pH and concentration	610:658	The composition of the mobile phase in terms of pH and concentration showed great influence on the chromatographic separation and was varied to allow the distinction of each CS without signal overlap for a total analysis time of 25 min.
34651208	4	56	theme	endocan	1092:1098	arg1	depolymerization					1065:1080	depolymerization	1065:1080	depolymerization of the CS endocan by chondroitinase lyase ABC	1065:1126	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	4	56	theme	endocan	1092:1098	arg1	desulfation					1002:1012	enzymatic desulfation	992:1012	enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase	992:1058	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	4	56	theme	endocan	1092:1098	arg1	transformations					961:975	various enzymatic transformations	943:975	various enzymatic transformations	943:975	This methodology was applied to determine the disaccharide composition of biological reaction media resulting from various enzymatic transformations of CS, such as enzymatic desulfation of CS disaccharides by a CS 4-O-endosulfatase, and depolymerization of the CS endocan by chondroitinase lyase ABC.
34651208	3	57	theme	25 min	820:825	arg1	time					812:815	a total analysis time	795:815	a total analysis time of 25 min	795:825	The composition of the mobile phase in terms of pH and concentration showed great influence on the chromatographic separation and was varied to allow the distinction of each CS without signal overlap for a total analysis time of 25 min.
33280848	6	0	theme	bacterial	1162:1170	arg1	community					1172:1180	the bacterial community	1158:1180	the bacterial community in the top, middle, and bottom layers	1158:1218	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	8	1	theme	"	1479:1479	arg1	structure					1481:1489	a "valley-peak" structure	1465:1489	a "valley-peak" structure within the layers	1465:1507	The distribution of aerobic and anaerobic bacteria showed a "valley-peak" structure within the layers.
33280848	6	2	theme	aerobic	1106:1112	arg1	bacteria					1114:1121	aerobic bacteria	1106:1121	aerobic bacteria	1106:1121	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	8	3	theme	aerobic	1427:1433	arg1	bacteria					1449:1456	aerobic and anaerobic bacteria	1427:1456	aerobic and anaerobic bacteria	1427:1456	The distribution of aerobic and anaerobic bacteria showed a "valley-peak" structure within the layers.
33280848	3	4	theme	linear	506:511	arg1	voltammetry					519:529	linear sweep voltammetry	506:529	linear sweep voltammetry (LSV)	506:535	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	3	4	theme	linear	506:511	arg1	LSV					532:534	LSV	532:534	LSV	532:534	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	10	5	from	study	1672:1676	arg1	useful					1688:1693	useful	1688:1693	useful	1688:1693	The results from this study should be useful to develop new catalyst and cathode in the MFC.
33280848	10	5	from	study	1672:1676	arg1	results					1654:1660	The results	1650:1660	The results from this study	1650:1676	The results from this study should be useful to develop new catalyst and cathode in the MFC.
33280848	3	6	theme	high-throughput	650:664	arg1	sequencing					666:675	high-throughput sequencing	650:675	high-throughput sequencing analysis on bacterial communities	650:709	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	7	7	theme	bacteria	1259:1266	arg1	amount					1239:1244	The amount	1235:1244	The amount of anaerobic bacteria in the top and middle layers (i.e., 52%, and 50% of the bacterial community, respectively)	1235:1357	The amount of anaerobic bacteria in the top and middle layers (i.e., 52%, and 50% of the bacterial community, respectively) was higher than that in the bottom layer (22%).
33280848	7	7	theme	bacteria	1259:1266	arg1	bacteria					1259:1266	anaerobic bacteria	1249:1266	anaerobic bacteria	1249:1266	The amount of anaerobic bacteria in the top and middle layers (i.e., 52%, and 50% of the bacterial community, respectively) was higher than that in the bottom layer (22%).
33280848	7	7	theme	bacteria	1259:1266	arg1	higher					1363:1368	higher	1363:1368	higher	1363:1368	The amount of anaerobic bacteria in the top and middle layers (i.e., 52%, and 50% of the bacterial community, respectively) was higher than that in the bottom layer (22%).
33280848	3	8	theme	bacterial	689:697	arg1	communities					699:709	bacterial communities	689:709	bacterial communities	689:709	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	5	9	theme	middle	1017:1022	arg1	layers					1058:1063	the bottom (0-50 μm), middle (50-100 μm), and top (100-150 μm) layers	995:1063	the bottom (0-50 μm), middle (50-100 μm), and top (100-150 μm) layers of the cathodic biofilm	995:1087	With different pH values, the composition and relative abundance of bacterial community significantly changed in the bottom (0-50 μm), middle (50-100 μm), and top (100-150 μm) layers of the cathodic biofilm.
33280848	3	10	theme	impedance	554:562	arg1	EIS					578:580	EIS	578:580	EIS	578:580	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	3	10	theme	impedance	554:562	arg1	spectroscopy					564:575	electrochemical impedance spectroscopy	538:575	electrochemical impedance spectroscopy (EIS)	538:581	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	6	11	from	middle	1194:1199	arg1	%					1153:1153	34%	1151:1153	34% of the bacterial community in the top, middle, and bottom layers	1151:1218	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	11	from	middle	1194:1199	arg1	community					1172:1180	the bacterial community	1158:1180	the bacterial community in the top, middle, and bottom layers	1158:1218	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	11	from	middle	1194:1199	arg1	%					1139:1139	12%	1137:1139	12%	1137:1139	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	11	from	middle	1194:1199	arg1	%					1144:1144	13%	1142:1144	13%	1142:1144	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	9	12	theme	high	1514:1517	arg1	concentration					1525:1537	The high CO32- concentration	1510:1537	The high CO32- concentration	1510:1537	The high CO32- concentration facilitates the hydroxyl transfer and the neutralization in the anode of the MFC under high alkali conditions.
33280848	5	13	dep	composition	912:922	arg1	the					908:910	the	908:910	the	908:910	With different pH values, the composition and relative abundance of bacterial community significantly changed in the bottom (0-50 μm), middle (50-100 μm), and top (100-150 μm) layers of the cathodic biofilm.
33280848	3	14	from	voltammetry	519:529	arg1	communities					699:709	bacterial communities	689:709	bacterial communities	689:709	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	4	15	from	densities	764:772	arg1	MFC					781:783	the MFC	777:783	the MFC	777:783	Results showed that the maximum power densities in the MFC increased with the pH values and reached 1221 ± 96 mW/m2 at pH = 10.5 during ∼30 d of operation.
33280848	5	16	theme	different	887:895	arg1	values					900:905	different pH values	887:905	different pH values	887:905	With different pH values, the composition and relative abundance of bacterial community significantly changed in the bottom (0-50 μm), middle (50-100 μm), and top (100-150 μm) layers of the cathodic biofilm.
33280848	6	17	from	%	1139:1139	arg1	top					1189:1191	the top	1185:1191	the top	1185:1191	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	17	from	%	1139:1139	arg1	middle					1194:1199	middle	1194:1199	middle	1194:1199	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	17	from	%	1139:1139	arg1	bottom					1206:1211	bottom	1206:1211	bottom	1206:1211	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	7	18	theme	bottom	1387:1392	arg1	layer					1394:1398	the bottom layer	1383:1398	the bottom layer (22%)	1383:1404	The amount of anaerobic bacteria in the top and middle layers (i.e., 52%, and 50% of the bacterial community, respectively) was higher than that in the bottom layer (22%).
33280848	7	18	theme	bottom	1387:1392	arg1	%					1403:1403	22%	1401:1403	22%	1401:1403	The amount of anaerobic bacteria in the top and middle layers (i.e., 52%, and 50% of the bacterial community, respectively) was higher than that in the bottom layer (22%).
33280848	2	19	theme	10.5	442:445	arg1	values					418:423	pH values	415:423	pH values	415:423	A single-chamber MFC with air-cathode was operated with 0.8 g/L maltodextrin and bicarbonate buffer solutions under pH values of 8.5, 9.5, and 10.5, respectively.
33280848	1	20	theme	cell	261:264	arg1	MFC					267:269	the microbial fuel cell (MFC)	242:270	the microbial fuel cell (MFC)	242:270	The aim of this study was to investigate pH effect on stratification of bacterial community in cathodic biofilm of the microbial fuel cell (MFC) under alkaline conditions.
33280848	0	21	from	Effect	0:5	arg1	distributions					26:38	bacterial distributions	16:38	bacterial distributions within cathodic biofilm of the microbial fuel cell with maltodextrin as the substrate	16:124	Effect of pH on bacterial distributions within cathodic biofilm of the microbial fuel cell with maltodextrin as the substrate.
33280848	0	22	theme	fuel	81:84	arg1	cell					86:89	the microbial fuel cell	67:89	the microbial fuel cell	67:89	Effect of pH on bacterial distributions within cathodic biofilm of the microbial fuel cell with maltodextrin as the substrate.
33280848	1	23	theme	alkaline	278:285	arg1	conditions					287:296	alkaline conditions	278:296	alkaline conditions	278:296	The aim of this study was to investigate pH effect on stratification of bacterial community in cathodic biofilm of the microbial fuel cell (MFC) under alkaline conditions.
33280848	5	24	theme	top	1041:1043	arg1	layers					1058:1063	the bottom (0-50 μm), middle (50-100 μm), and top (100-150 μm) layers	995:1063	the bottom (0-50 μm), middle (50-100 μm), and top (100-150 μm) layers of the cathodic biofilm	995:1087	With different pH values, the composition and relative abundance of bacterial community significantly changed in the bottom (0-50 μm), middle (50-100 μm), and top (100-150 μm) layers of the cathodic biofilm.
33280848	1	25	theme	microbial	246:254	arg1	MFC					267:269	the microbial fuel cell (MFC)	242:270	the microbial fuel cell (MFC)	242:270	The aim of this study was to investigate pH effect on stratification of bacterial community in cathodic biofilm of the microbial fuel cell (MFC) under alkaline conditions.
33280848	2	26	theme	pH	415:416	arg1	values					418:423	pH values	415:423	pH values	415:423	A single-chamber MFC with air-cathode was operated with 0.8 g/L maltodextrin and bicarbonate buffer solutions under pH values of 8.5, 9.5, and 10.5, respectively.
33280848	0	27	theme	pH	10:11	arg1	Effect					0:5	Effect	0:5	Effect of pH on bacterial distributions within cathodic biofilm of the microbial fuel cell with maltodextrin as the substrate.	0:125	Effect of pH on bacterial distributions within cathodic biofilm of the microbial fuel cell with maltodextrin as the substrate.
33280848	6	28	from	bottom	1206:1211	arg1	%					1153:1153	34%	1151:1153	34% of the bacterial community in the top, middle, and bottom layers	1151:1218	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	28	from	bottom	1206:1211	arg1	community					1172:1180	the bacterial community	1158:1180	the bacterial community in the top, middle, and bottom layers	1158:1218	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	28	from	bottom	1206:1211	arg1	%					1139:1139	12%	1137:1139	12%	1137:1139	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	28	from	bottom	1206:1211	arg1	%					1144:1144	13%	1142:1144	13%	1142:1144	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	3	29	theme	scanning	599:606	arg1	CLSM					620:623	CLSM	620:623	CLSM	620:623	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	3	29	theme	scanning	599:606	arg1	microscopy					608:617	confocal laser scanning microscopy	584:617	confocal laser scanning microscopy (CLSM)	584:624	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	2	30	theme	bicarbonate	380:390	arg1	solutions					399:407	bicarbonate buffer solutions	380:407	bicarbonate buffer solutions	380:407	A single-chamber MFC with air-cathode was operated with 0.8 g/L maltodextrin and bicarbonate buffer solutions under pH values of 8.5, 9.5, and 10.5, respectively.
33280848	0	31	theme	bacterial	16:24	arg1	distributions					26:38	bacterial distributions	16:38	bacterial distributions within cathodic biofilm of the microbial fuel cell with maltodextrin as the substrate	16:124	Effect of pH on bacterial distributions within cathodic biofilm of the microbial fuel cell with maltodextrin as the substrate.
33280848	1	32	theme	MFC	267:269	arg1	biofilm					231:237	cathodic biofilm	222:237	cathodic biofilm of the microbial fuel cell (MFC)	222:270	The aim of this study was to investigate pH effect on stratification of bacterial community in cathodic biofilm of the microbial fuel cell (MFC) under alkaline conditions.
33280848	9	33	theme	MFC	1616:1618	arg1	anode					1603:1607	the anode	1599:1607	the anode of the MFC	1599:1618	The high CO32- concentration facilitates the hydroxyl transfer and the neutralization in the anode of the MFC under high alkali conditions.
33280848	9	34	from	transfer	1564:1571	arg1	anode					1603:1607	the anode	1599:1607	the anode of the MFC	1599:1618	The high CO32- concentration facilitates the hydroxyl transfer and the neutralization in the anode of the MFC under high alkali conditions.
33280848	3	35	theme	confocal	584:591	arg1	CLSM					620:623	CLSM	620:623	CLSM	620:623	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	3	35	theme	confocal	584:591	arg1	microscopy					608:617	confocal laser scanning microscopy	584:617	confocal laser scanning microscopy (CLSM)	584:624	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	10	36	theme	new	1706:1708	arg1	catalyst					1710:1717	new catalyst	1706:1717	new catalyst	1706:1717	The results from this study should be useful to develop new catalyst and cathode in the MFC.
33280848	2	37	theme	single-chamber	301:314	arg1	MFC					316:318	A single-chamber MFC	299:318	A single-chamber MFC with air-cathode	299:335	A single-chamber MFC with air-cathode was operated with 0.8 g/L maltodextrin and bicarbonate buffer solutions under pH values of 8.5, 9.5, and 10.5, respectively.
33280848	5	38	theme	biofilm	1081:1087	arg1	layers					1058:1063	the bottom (0-50 μm), middle (50-100 μm), and top (100-150 μm) layers	995:1063	the bottom (0-50 μm), middle (50-100 μm), and top (100-150 μm) layers of the cathodic biofilm	995:1087	With different pH values, the composition and relative abundance of bacterial community significantly changed in the bottom (0-50 μm), middle (50-100 μm), and top (100-150 μm) layers of the cathodic biofilm.
33280848	7	39	theme	bacterial	1324:1332	arg1	community					1334:1342	the bacterial community	1320:1342	the bacterial community	1320:1342	The amount of anaerobic bacteria in the top and middle layers (i.e., 52%, and 50% of the bacterial community, respectively) was higher than that in the bottom layer (22%).
33280848	5	40	theme	bottom	999:1004	arg1	layers					1058:1063	the bottom (0-50 μm), middle (50-100 μm), and top (100-150 μm) layers	995:1063	the bottom (0-50 μm), middle (50-100 μm), and top (100-150 μm) layers of the cathodic biofilm	995:1087	With different pH values, the composition and relative abundance of bacterial community significantly changed in the bottom (0-50 μm), middle (50-100 μm), and top (100-150 μm) layers of the cathodic biofilm.
33280848	9	41	theme	alkali	1631:1636	arg1	conditions					1638:1647	high alkali conditions	1626:1647	high alkali conditions	1626:1647	The high CO32- concentration facilitates the hydroxyl transfer and the neutralization in the anode of the MFC under high alkali conditions.
33280848	4	42	theme	maximum	750:756	arg1	densities					764:772	the maximum power densities	746:772	the maximum power densities in the MFC	746:783	Results showed that the maximum power densities in the MFC increased with the pH values and reached 1221 ± 96 mW/m2 at pH = 10.5 during ∼30 d of operation.
33280848	5	43	theme	bacterial	950:958	arg1	community					960:968	bacterial community	950:968	bacterial community	950:968	With different pH values, the composition and relative abundance of bacterial community significantly changed in the bottom (0-50 μm), middle (50-100 μm), and top (100-150 μm) layers of the cathodic biofilm.
33280848	3	44	from	microscopy	608:617	arg1	communities					699:709	bacterial communities	689:709	bacterial communities	689:709	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	3	45	theme	cathodic	466:473	arg1	biofilms					475:482	The cathodic biofilms	462:482	The cathodic biofilms	462:482	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	8	46	theme	bacteria	1449:1456	arg1	distribution					1411:1422	The distribution	1407:1422	The distribution of aerobic and anaerobic bacteria	1407:1456	The distribution of aerobic and anaerobic bacteria showed a "valley-peak" structure within the layers.
33280848	6	47	from	community	1172:1180	arg1	top					1189:1191	the top	1185:1191	the top	1185:1191	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	47	from	community	1172:1180	arg1	middle					1194:1199	middle	1194:1199	middle	1194:1199	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	47	from	community	1172:1180	arg1	bottom					1206:1211	bottom	1206:1211	bottom	1206:1211	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	48	theme	community	1172:1180	arg1	%					1153:1153	34%	1151:1153	34% of the bacterial community in the top, middle, and bottom layers	1151:1218	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	48	theme	community	1172:1180	arg1	community					1172:1180	the bacterial community	1158:1180	the bacterial community in the top, middle, and bottom layers	1158:1218	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	48	theme	community	1172:1180	arg1	%					1139:1139	12%	1137:1139	12%	1137:1139	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	48	theme	community	1172:1180	arg1	%					1144:1144	13%	1142:1144	13%	1142:1144	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	1	49	theme	cathodic	222:229	arg1	biofilm					231:237	cathodic biofilm	222:237	cathodic biofilm of the microbial fuel cell (MFC)	222:270	The aim of this study was to investigate pH effect on stratification of bacterial community in cathodic biofilm of the microbial fuel cell (MFC) under alkaline conditions.
33280848	3	50	theme	freezing	627:634	arg1	microtome					636:644	freezing microtome	627:644	freezing microtome	627:644	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	8	51	theme	valley-peak	1468:1478	arg1	structure					1481:1489	a "valley-peak" structure	1465:1489	a "valley-peak" structure within the layers	1465:1507	The distribution of aerobic and anaerobic bacteria showed a "valley-peak" structure within the layers.
33280848	6	52	from	%	1153:1153	arg1	top					1189:1191	the top	1185:1191	the top	1185:1191	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	52	from	%	1153:1153	arg1	middle					1194:1199	middle	1194:1199	middle	1194:1199	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	52	from	%	1153:1153	arg1	bottom					1206:1211	bottom	1206:1211	bottom	1206:1211	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	0	53	with	biofilm	56:62	arg1	maltodextrin					96:107	maltodextrin	96:107	maltodextrin as the substrate	96:124	Effect of pH on bacterial distributions within cathodic biofilm of the microbial fuel cell with maltodextrin as the substrate.
33280848	1	54	theme	study	143:147	arg1	aim					131:133	The aim	127:133	The aim of this study	127:147	The aim of this study was to investigate pH effect on stratification of bacterial community in cathodic biofilm of the microbial fuel cell (MFC) under alkaline conditions.
33280848	9	55	theme	high	1626:1629	arg1	conditions					1638:1647	high alkali conditions	1626:1647	high alkali conditions	1626:1647	The high CO32- concentration facilitates the hydroxyl transfer and the neutralization in the anode of the MFC under high alkali conditions.
33280848	7	56	theme	middle	1283:1288	arg1	layers					1290:1295	middle layers	1283:1295	middle layers (i.e., 52%, and 50% of the bacterial community, respectively)	1283:1357	The amount of anaerobic bacteria in the top and middle layers (i.e., 52%, and 50% of the bacterial community, respectively) was higher than that in the bottom layer (22%).
33280848	3	57	theme	sequencing	666:675	arg1	analysis					677:684	high-throughput sequencing analysis	650:684	high-throughput sequencing analysis on bacterial communities	650:709	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	4	58	theme	pH	804:805	arg1	values					807:812	the pH values	800:812	the pH values	800:812	Results showed that the maximum power densities in the MFC increased with the pH values and reached 1221 ± 96 mW/m2 at pH = 10.5 during ∼30 d of operation.
33280848	3	59	from	analysis	677:684	arg1	communities					699:709	bacterial communities	689:709	bacterial communities	689:709	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	3	60	theme	sweep	513:517	arg1	voltammetry					519:529	linear sweep voltammetry	506:529	linear sweep voltammetry (LSV)	506:535	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	3	60	theme	sweep	513:517	arg1	LSV					532:534	LSV	532:534	LSV	532:534	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	1	61	theme	pH	168:169	arg1	effect					171:176	pH effect	168:176	pH effect on stratification of bacterial community in cathodic biofilm of the microbial fuel cell (MFC) under alkaline conditions	168:296	The aim of this study was to investigate pH effect on stratification of bacterial community in cathodic biofilm of the microbial fuel cell (MFC) under alkaline conditions.
33280848	6	62	dep	top	1189:1191	arg1	layers					1213:1218	layers	1213:1218	layers	1213:1218	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	4	63	theme	operation	871:879	arg1	∼30 d					862:866	∼30 d	862:866	∼30 d of operation	862:879	Results showed that the maximum power densities in the MFC increased with the pH values and reached 1221 ± 96 mW/m2 at pH = 10.5 during ∼30 d of operation.
33280848	3	64	theme	electrochemical	538:552	arg1	EIS					578:580	EIS	578:580	EIS	578:580	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	3	64	theme	electrochemical	538:552	arg1	spectroscopy					564:575	electrochemical impedance spectroscopy	538:575	electrochemical impedance spectroscopy (EIS)	538:581	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	7	65	theme	anaerobic	1249:1257	arg1	bacteria					1259:1266	anaerobic bacteria	1249:1266	anaerobic bacteria	1249:1266	The amount of anaerobic bacteria in the top and middle layers (i.e., 52%, and 50% of the bacterial community, respectively) was higher than that in the bottom layer (22%).
33280848	6	66	from	top	1189:1191	arg1	%					1153:1153	34%	1151:1153	34% of the bacterial community in the top, middle, and bottom layers	1151:1218	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	66	from	top	1189:1191	arg1	community					1172:1180	the bacterial community	1158:1180	the bacterial community in the top, middle, and bottom layers	1158:1218	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	66	from	top	1189:1191	arg1	%					1139:1139	12%	1137:1139	12%	1137:1139	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	66	from	top	1189:1191	arg1	%					1144:1144	13%	1142:1144	13%	1142:1144	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	3	67	from	microtome	636:644	arg1	communities					699:709	bacterial communities	689:709	bacterial communities	689:709	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	1	68	from	stratification	181:194	arg1	biofilm					231:237	cathodic biofilm	222:237	cathodic biofilm of the microbial fuel cell (MFC)	222:270	The aim of this study was to investigate pH effect on stratification of bacterial community in cathodic biofilm of the microbial fuel cell (MFC) under alkaline conditions.
33280848	2	69	with	MFC	316:318	arg1	air-cathode					325:335	air-cathode	325:335	air-cathode	325:335	A single-chamber MFC with air-cathode was operated with 0.8 g/L maltodextrin and bicarbonate buffer solutions under pH values of 8.5, 9.5, and 10.5, respectively.
33280848	1	70	from	effect	171:176	arg1	stratification					181:194	stratification	181:194	stratification of bacterial community in cathodic biofilm of the microbial fuel cell (MFC)	181:270	The aim of this study was to investigate pH effect on stratification of bacterial community in cathodic biofilm of the microbial fuel cell (MFC) under alkaline conditions.
33280848	9	71	theme	CO32-	1519:1523	arg1	concentration					1525:1537	The high CO32- concentration	1510:1537	The high CO32- concentration	1510:1537	The high CO32- concentration facilitates the hydroxyl transfer and the neutralization in the anode of the MFC under high alkali conditions.
33280848	6	72	from	%	1144:1144	arg1	top					1189:1191	the top	1185:1191	the top	1185:1191	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	72	from	%	1144:1144	arg1	middle					1194:1199	middle	1194:1199	middle	1194:1199	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	6	72	from	%	1144:1144	arg1	bottom					1206:1211	bottom	1206:1211	bottom	1206:1211	With pH = 10.5, aerobic bacteria accounted for 12%, 13%, and 34% of the bacterial community in the top, middle, and bottom layers, respectively.
33280848	4	73	from	pH = 10.5	845:853	arg1	1221 ± 96 mW/m2					826:840	1221 ± 96 mW/m2	826:840	1221 ± 96 mW/m2 at pH = 10.5	826:853	Results showed that the maximum power densities in the MFC increased with the pH values and reached 1221 ± 96 mW/m2 at pH = 10.5 during ∼30 d of operation.
33280848	0	74	theme	microbial	71:79	arg1	cell					86:89	the microbial fuel cell	67:89	the microbial fuel cell	67:89	Effect of pH on bacterial distributions within cathodic biofilm of the microbial fuel cell with maltodextrin as the substrate.
33280848	9	75	theme	hydroxyl	1555:1562	arg1	transfer					1564:1571	the hydroxyl transfer	1551:1571	the hydroxyl transfer	1551:1571	The high CO32- concentration facilitates the hydroxyl transfer and the neutralization in the anode of the MFC under high alkali conditions.
33280848	0	76	theme	cell	86:89	arg1	biofilm					56:62	cathodic biofilm	47:62	cathodic biofilm of the microbial fuel cell with maltodextrin as the substrate	47:124	Effect of pH on bacterial distributions within cathodic biofilm of the microbial fuel cell with maltodextrin as the substrate.
33280848	3	77	from	spectroscopy	564:575	arg1	communities					699:709	bacterial communities	689:709	bacterial communities	689:709	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	5	78	theme	relative	928:935	arg1	abundance					937:945	relative abundance	928:945	relative abundance	928:945	With different pH values, the composition and relative abundance of bacterial community significantly changed in the bottom (0-50 μm), middle (50-100 μm), and top (100-150 μm) layers of the cathodic biofilm.
33280848	2	79	theme	0.8 g/L	355:361	arg1	maltodextrin					363:374	0.8 g/L maltodextrin	355:374	0.8 g/L maltodextrin	355:374	A single-chamber MFC with air-cathode was operated with 0.8 g/L maltodextrin and bicarbonate buffer solutions under pH values of 8.5, 9.5, and 10.5, respectively.
33280848	5	80	theme	pH	897:898	arg1	values					900:905	different pH values	887:905	different pH values	887:905	With different pH values, the composition and relative abundance of bacterial community significantly changed in the bottom (0-50 μm), middle (50-100 μm), and top (100-150 μm) layers of the cathodic biofilm.
33280848	1	81	theme	fuel	256:259	arg1	MFC					267:269	the microbial fuel cell (MFC)	242:270	the microbial fuel cell (MFC)	242:270	The aim of this study was to investigate pH effect on stratification of bacterial community in cathodic biofilm of the microbial fuel cell (MFC) under alkaline conditions.
33280848	7	82	from	amount	1239:1244	arg1	top					1275:1277	the top	1271:1277	the top	1271:1277	The amount of anaerobic bacteria in the top and middle layers (i.e., 52%, and 50% of the bacterial community, respectively) was higher than that in the bottom layer (22%).
33280848	7	82	from	amount	1239:1244	arg1	layers					1290:1295	middle layers	1283:1295	middle layers (i.e., 52%, and 50% of the bacterial community, respectively)	1283:1357	The amount of anaerobic bacteria in the top and middle layers (i.e., 52%, and 50% of the bacterial community, respectively) was higher than that in the bottom layer (22%).
33280848	3	83	theme	laser	593:597	arg1	CLSM					620:623	CLSM	620:623	CLSM	620:623	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	3	83	theme	laser	593:597	arg1	microscopy					608:617	confocal laser scanning microscopy	584:617	confocal laser scanning microscopy (CLSM)	584:624	The cathodic biofilms were characterized by linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS), confocal laser scanning microscopy (CLSM), freezing microtome and high-throughput sequencing analysis on bacterial communities, respectively.
33280848	2	84	theme	buffer	392:397	arg1	solutions					399:407	bicarbonate buffer solutions	380:407	bicarbonate buffer solutions	380:407	A single-chamber MFC with air-cathode was operated with 0.8 g/L maltodextrin and bicarbonate buffer solutions under pH values of 8.5, 9.5, and 10.5, respectively.
33280848	9	85	from	neutralization	1581:1594	arg1	anode					1603:1607	the anode	1599:1607	the anode of the MFC	1599:1618	The high CO32- concentration facilitates the hydroxyl transfer and the neutralization in the anode of the MFC under high alkali conditions.
33280848	2	86	theme	9.5	433:435	arg1	values					418:423	pH values	415:423	pH values	415:423	A single-chamber MFC with air-cathode was operated with 0.8 g/L maltodextrin and bicarbonate buffer solutions under pH values of 8.5, 9.5, and 10.5, respectively.
33280848	1	87	theme	community	209:217	arg1	stratification					181:194	stratification	181:194	stratification of bacterial community in cathodic biofilm of the microbial fuel cell (MFC)	181:270	The aim of this study was to investigate pH effect on stratification of bacterial community in cathodic biofilm of the microbial fuel cell (MFC) under alkaline conditions.
33280848	0	88	theme	cathodic	47:54	arg1	biofilm					56:62	cathodic biofilm	47:62	cathodic biofilm of the microbial fuel cell with maltodextrin as the substrate	47:124	Effect of pH on bacterial distributions within cathodic biofilm of the microbial fuel cell with maltodextrin as the substrate.
33280848	5	89	theme	cathodic	1072:1079	arg1	biofilm					1081:1087	the cathodic biofilm	1068:1087	the cathodic biofilm	1068:1087	With different pH values, the composition and relative abundance of bacterial community significantly changed in the bottom (0-50 μm), middle (50-100 μm), and top (100-150 μm) layers of the cathodic biofilm.
33280848	2	90	theme	8.5	428:430	arg1	values					418:423	pH values	415:423	pH values	415:423	A single-chamber MFC with air-cathode was operated with 0.8 g/L maltodextrin and bicarbonate buffer solutions under pH values of 8.5, 9.5, and 10.5, respectively.
33280848	8	91	theme	anaerobic	1439:1447	arg1	bacteria					1449:1456	aerobic and anaerobic bacteria	1427:1456	aerobic and anaerobic bacteria	1427:1456	The distribution of aerobic and anaerobic bacteria showed a "valley-peak" structure within the layers.
33280848	7	92	dep	%	1306:1306	arg1	i.e.					1298:1301	i.e.	1298:1301	i.e.	1298:1301	The amount of anaerobic bacteria in the top and middle layers (i.e., 52%, and 50% of the bacterial community, respectively) was higher than that in the bottom layer (22%).
33280848	7	93	theme	community	1334:1342	arg1	community					1334:1342	the bacterial community	1320:1342	the bacterial community	1320:1342	The amount of anaerobic bacteria in the top and middle layers (i.e., 52%, and 50% of the bacterial community, respectively) was higher than that in the bottom layer (22%).
33280848	7	93	theme	community	1334:1342	arg1	%					1306:1306	52%	1304:1306	52%	1304:1306	The amount of anaerobic bacteria in the top and middle layers (i.e., 52%, and 50% of the bacterial community, respectively) was higher than that in the bottom layer (22%).
33280848	7	93	theme	community	1334:1342	arg1	%					1315:1315	50%	1313:1315	50% of the bacterial community	1313:1342	The amount of anaerobic bacteria in the top and middle layers (i.e., 52%, and 50% of the bacterial community, respectively) was higher than that in the bottom layer (22%).
33280848	1	94	theme	bacterial	199:207	arg1	community					209:217	bacterial community	199:217	bacterial community	199:217	The aim of this study was to investigate pH effect on stratification of bacterial community in cathodic biofilm of the microbial fuel cell (MFC) under alkaline conditions.
33280848	5	95	theme	community	960:968	arg1	composition					912:922	composition	912:922	composition	912:922	With different pH values, the composition and relative abundance of bacterial community significantly changed in the bottom (0-50 μm), middle (50-100 μm), and top (100-150 μm) layers of the cathodic biofilm.
33280848	5	95	theme	community	960:968	arg1	abundance					937:945	relative abundance	928:945	relative abundance	928:945	With different pH values, the composition and relative abundance of bacterial community significantly changed in the bottom (0-50 μm), middle (50-100 μm), and top (100-150 μm) layers of the cathodic biofilm.
33280848	4	96	theme	power	758:762	arg1	densities					764:772	the maximum power densities	746:772	the maximum power densities in the MFC	746:783	Results showed that the maximum power densities in the MFC increased with the pH values and reached 1221 ± 96 mW/m2 at pH = 10.5 during ∼30 d of operation.
33049125	7	0	theme	better	1518:1523	arg1	ratio					1525:1529	a better ratio	1516:1529	a better ratio of bone volume to the total volume for BM-MSC-laden composites over scaffold-only and defect-only groups	1516:1634	In addition, osteogenesis in rat tibial model over 6 weeks confirmed a better ratio of bone volume to the total volume for BM-MSC-laden composites over scaffold-only and defect-only groups.
33049125	8	1	theme	bone	1901:1904	arg1	potential					1841:1849	the potential	1837:1849	the potential of CHT/HA/PCL composite	1837:1873	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	8	1	theme	bone	1901:1904	arg1	analog					1906:1911	a potential cancellous bone analog	1878:1911	a potential cancellous bone analog	1878:1911	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	8	2	dep	in	1779:1780	arg1	vitro					1782:1786	vitro	1782:1786	vitro	1782:1786	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	4	3	theme	human	650:654	arg1	BM-MSCs					682:688	BM-MSCs	682:688	BM-MSCs	682:688	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	4	3	theme	human	650:654	arg1	MSCs					676:679	human bone marrow-derived MSCs	650:679	human bone marrow-derived MSCs (BM-MSCs)	650:689	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	6	4	theme	enhanced	1297:1304	arg1	functionality					1311:1323	The enhanced cell functionality	1293:1323	The enhanced cell functionality on CHT/HA/PCL composites	1293:1348	The enhanced cell functionality on CHT/HA/PCL composites was explained in terms of interplay among the surface properties and the optimal source of MSCs.
33049125	5	5	theme	terminal	1224:1231	arg1	differentiation					1233:1247	terminal differentiation	1224:1247	terminal differentiation	1224:1247	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	2	6	theme	quantitative	324:335	arg1	analysis					337:344	quantitative analysis	324:344	quantitative analysis of early osseointegration of highly porous scaffolds	324:397	However, the understanding of the stem cell progenitor-dependent cell functionality modulation and quantitative analysis of early osseointegration of highly porous scaffolds have not been explored.
33049125	7	7	theme	tibial	1480:1485	arg1	model					1487:1491	rat tibial model	1476:1491	rat tibial model	1476:1491	In addition, osteogenesis in rat tibial model over 6 weeks confirmed a better ratio of bone volume to the total volume for BM-MSC-laden composites over scaffold-only and defect-only groups.
33049125	5	8	theme	scanning	944:951	arg1	expression					989:998	real-time gene expression	974:998	real-time gene expression (alkaline phosphatase [ALP]	974:1026	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	8	theme	scanning	944:951	arg1	microscopy					962:971	scanning electron microscopy	944:971	scanning electron microscopy	944:971	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	2	9	theme	osseointegration	355:370	arg1	understanding					238:250	the understanding	234:250	the understanding of the stem cell progenitor-dependent cell functionality modulation	234:318	However, the understanding of the stem cell progenitor-dependent cell functionality modulation and quantitative analysis of early osseointegration of highly porous scaffolds have not been explored.
33049125	2	9	theme	osseointegration	355:370	arg1	analysis					337:344	quantitative analysis	324:344	quantitative analysis of early osseointegration of highly porous scaffolds	324:397	However, the understanding of the stem cell progenitor-dependent cell functionality modulation and quantitative analysis of early osseointegration of highly porous scaffolds have not been explored.
33049125	8	10	theme	controlled	1768:1777	arg1	degradation					1788:1798	controlled in vitro degradation	1768:1798	controlled in vitro degradation	1768:1798	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	4	11	theme	adipose-derived	692:706	arg1	AD-MSCs					714:720	AD-MSCs	714:720	AD-MSCs	714:720	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	4	11	theme	adipose-derived	692:706	arg1	MSCs					708:711	adipose-derived MSCs	692:711	adipose-derived MSCs (AD-MSCs)	692:721	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	5	12	from	osteogenesis	1206:1217	arg1	composites					1276:1285	BM-MSC-laden CHT/HA/PCL composites	1252:1285	BM-MSC-laden CHT/HA/PCL composites	1252:1285	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	2	13	theme	cell	264:267	arg1	modulation					309:318	the stem cell progenitor-dependent cell functionality modulation	255:318	the stem cell progenitor-dependent cell functionality modulation	255:318	However, the understanding of the stem cell progenitor-dependent cell functionality modulation and quantitative analysis of early osseointegration of highly porous scaffolds have not been explored.
33049125	5	14	theme	WJ-MSC-laden	908:919	arg1	composites					932:941	WJ-MSC-laden CHT/HA/PCL composites	908:941	WJ-MSC-laden CHT/HA/PCL composites	908:941	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	1	15	theme	freeze-drying	150:162	arg1	process					164:170	freeze-drying process	150:170	freeze-drying process	150:170	Among conventional fabrication techniques, freeze-drying process has widely been investigated for polymeric implants.
33049125	4	16	link	adipose-derived	692:706	arg1	AD-MSCs					714:720	AD-MSCs	714:720	AD-MSCs	714:720	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	4	16	link	adipose-derived	692:706	arg1	MSCs					708:711	adipose-derived MSCs	692:711	adipose-derived MSCs (AD-MSCs)	692:721	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	6	17	theme	CHT/HA/PCL	1328:1337	arg1	composites					1339:1348	CHT/HA/PCL composites	1328:1348	CHT/HA/PCL composites	1328:1348	The enhanced cell functionality on CHT/HA/PCL composites was explained in terms of interplay among the surface properties and the optimal source of MSCs.
33049125	2	18	theme	modulation	309:318	arg1	understanding					238:250	the understanding	234:250	the understanding of the stem cell progenitor-dependent cell functionality modulation	234:318	However, the understanding of the stem cell progenitor-dependent cell functionality modulation and quantitative analysis of early osseointegration of highly porous scaffolds have not been explored.
33049125	2	18	theme	modulation	309:318	arg1	analysis					337:344	quantitative analysis	324:344	quantitative analysis of early osseointegration of highly porous scaffolds	324:397	However, the understanding of the stem cell progenitor-dependent cell functionality modulation and quantitative analysis of early osseointegration of highly porous scaffolds have not been explored.
33049125	5	19	theme	alkaline	1001:1008	arg1	expression					989:998	real-time gene expression	974:998	real-time gene expression (alkaline phosphatase [ALP]	974:1026	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	19	theme	alkaline	1001:1008	arg1	[ALP					1022:1025	alkaline phosphatase [ALP	1001:1025	alkaline phosphatase [ALP	1001:1025	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	6	20	theme	optimal	1423:1429	arg1	source					1431:1436	the optimal source	1419:1436	the optimal source of MSCs	1419:1444	The enhanced cell functionality on CHT/HA/PCL composites was explained in terms of interplay among the surface properties and the optimal source of MSCs.
33049125	2	21	theme	cell	290:293	arg1	modulation					309:318	the stem cell progenitor-dependent cell functionality modulation	255:318	the stem cell progenitor-dependent cell functionality modulation	255:318	However, the understanding of the stem cell progenitor-dependent cell functionality modulation and quantitative analysis of early osseointegration of highly porous scaffolds have not been explored.
33049125	8	22	theme	porous	1681:1686	arg1	architecture					1688:1699	3D porous architecture	1678:1699	3D porous architecture	1678:1699	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	5	23	dep	demonstrated	1182:1193	arg1	osteocalcin					1054:1064	osteocalcin	1054:1064	osteocalcin	1054:1064	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	23	dep	demonstrated	1182:1193	arg1	morphogenetic					1082:1094	morphogenetic	1082:1094	morphogenetic	1082:1094	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	23	dep	demonstrated	1182:1193	arg1	collagen					1029:1036	collagen	1029:1036	collagen	1029:1036	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	23	dep	demonstrated	1182:1193	arg1	protein					1096:1102	protein	1096:1102	protein	1096:1102	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	23	dep	demonstrated	1182:1193	arg1	type					1038:1041	type	1038:1041	type	1038:1041	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	23	dep	demonstrated	1182:1193	arg1	4					1104:1104	4	1104:1104	4	1104:1104	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	23	dep	demonstrated	1182:1193	arg1	I					1043:1043	I	1043:1043	I	1043:1043	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	23	dep	demonstrated	1182:1193	arg1	[OCN					1066:1069	[OCN	1066:1069	[OCN	1066:1069	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	23	dep	demonstrated	1182:1193	arg1	[Col					1045:1048	[Col	1045:1048	[Col	1045:1048	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	23	dep	demonstrated	1182:1193	arg1	[BMP-4					1106:1111	[BMP-4	1106:1111	[BMP-4	1106:1111	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	23	dep	demonstrated	1182:1193	arg1	I					1050:1050	I	1050:1050	I	1050:1050	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	24	theme	activity	807:814	arg1	assay					880:884	2,5 diphenyl-2H-tetrazoliumbromide) assay	844:884	2,5 diphenyl-2H-tetrazoliumbromide) assay	844:884	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	24	theme	activity	807:814	arg1	[3-4,5-dimethylthiazol-2yl-					816:842	metabolic activity [3-4,5-dimethylthiazol-2yl-	797:842	metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay]	797:885	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	25	theme	real-time	974:982	arg1	expression					989:998	real-time gene expression	974:998	real-time gene expression (alkaline phosphatase [ALP]	974:1026	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	25	theme	real-time	974:982	arg1	[ALP					1022:1025	alkaline phosphatase [ALP	1001:1025	alkaline phosphatase [ALP	1001:1025	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	25	theme	real-time	974:982	arg1	microscopy					962:971	scanning electron microscopy	944:971	scanning electron microscopy	944:971	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	7	26	from	osteogenesis	1460:1471	arg1	model					1487:1491	rat tibial model	1476:1491	rat tibial model	1476:1491	In addition, osteogenesis in rat tibial model over 6 weeks confirmed a better ratio of bone volume to the total volume for BM-MSC-laden composites over scaffold-only and defect-only groups.
33049125	6	27	theme	surface	1396:1402	arg1	properties					1404:1413	the surface properties	1392:1413	the surface properties	1392:1413	The enhanced cell functionality on CHT/HA/PCL composites was explained in terms of interplay among the surface properties and the optimal source of MSCs.
33049125	8	28	theme	composite	1865:1873	arg1	potential					1841:1849	the potential	1837:1849	the potential of CHT/HA/PCL composite	1837:1873	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	8	28	theme	composite	1865:1873	arg1	analog					1906:1911	a potential cancellous bone analog	1878:1911	a potential cancellous bone analog	1878:1911	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	7	29	theme	BM-MSC-laden	1570:1581	arg1	composites					1583:1592	BM-MSC-laden composites	1570:1592	BM-MSC-laden composites	1570:1592	In addition, osteogenesis in rat tibial model over 6 weeks confirmed a better ratio of bone volume to the total volume for BM-MSC-laden composites over scaffold-only and defect-only groups.
33049125	5	30	theme	cell	777:780	arg1	attachment					782:791	cell attachment	777:791	cell attachment	777:791	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	0	31	from	osteogenesis	48:59	arg1	analogs					98:104	highly porous chitosan-based bone analogs	64:104	highly porous chitosan-based bone analogs	64:104	Tissue-specific mesenchymal stem cell-dependent osteogenesis in highly porous chitosan-based bone analogs.
33049125	8	32	theme	cancellous	1890:1899	arg1	potential					1841:1849	the potential	1837:1849	the potential of CHT/HA/PCL composite	1837:1873	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	8	32	theme	cancellous	1890:1899	arg1	analog					1906:1911	a potential cancellous bone analog	1878:1911	a potential cancellous bone analog	1878:1911	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	1	33	theme	conventional	113:124	arg1	techniques					138:147	conventional fabrication techniques	113:147	conventional fabrication techniques	113:147	Among conventional fabrication techniques, freeze-drying process has widely been investigated for polymeric implants.
33049125	4	34	dep	in	550:551	arg1	vitro					553:557	vitro	553:557	vitro	553:557	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	7	35	theme	total	1553:1557	arg1	volume					1559:1564	the total volume	1549:1564	the total volume for BM-MSC-laden composites over scaffold-only and defect-only groups	1549:1634	In addition, osteogenesis in rat tibial model over 6 weeks confirmed a better ratio of bone volume to the total volume for BM-MSC-laden composites over scaffold-only and defect-only groups.
33049125	5	36	theme	CHT/HA/PCL	921:930	arg1	composites					932:941	WJ-MSC-laden CHT/HA/PCL composites	908:941	WJ-MSC-laden CHT/HA/PCL composites	908:941	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	4	37	link	marrow-derived	661:674	arg1	BM-MSCs					682:688	BM-MSCs	682:688	BM-MSCs	682:688	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	4	37	link	marrow-derived	661:674	arg1	MSCs					676:679	human bone marrow-derived MSCs	650:679	human bone marrow-derived MSCs (BM-MSCs)	650:689	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	4	38	theme	mesenchymal	593:603	arg1	cells					610:614	mesenchymal stem cells	593:614	mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs)	593:765	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	4	38	theme	mesenchymal	593:603	arg1	MSCs					617:620	MSCs	617:620	MSCs	617:620	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	0	39	theme	chitosan-based	78:91	arg1	analogs					98:104	highly porous chitosan-based bone analogs	64:104	highly porous chitosan-based bone analogs	64:104	Tissue-specific mesenchymal stem cell-dependent osteogenesis in highly porous chitosan-based bone analogs.
33049125	5	40	theme	diphenyl-2H-tetrazoliumbromide	848:877	arg1	assay					880:884	2,5 diphenyl-2H-tetrazoliumbromide) assay	844:884	2,5 diphenyl-2H-tetrazoliumbromide) assay	844:884	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	40	theme	diphenyl-2H-tetrazoliumbromide	848:877	arg1	[3-4,5-dimethylthiazol-2yl-					816:842	metabolic activity [3-4,5-dimethylthiazol-2yl-	797:842	metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay]	797:885	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	7	41	theme	bone	1534:1537	arg1	volume					1539:1544	bone volume	1534:1544	bone volume	1534:1544	In addition, osteogenesis in rat tibial model over 6 weeks confirmed a better ratio of bone volume to the total volume for BM-MSC-laden composites over scaffold-only and defect-only groups.
33049125	5	42	dep	immunostaining	1120:1133	arg1	OCN					1154:1156	OCN	1154:1156	OCN	1154:1156	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	42	dep	immunostaining	1120:1133	arg1	β-CATENIN					1143:1151	β-CATENIN	1143:1151	β-CATENIN	1143:1151	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	42	dep	immunostaining	1120:1133	arg1	I					1140:1140	COL I	1136:1140	COL I	1136:1140	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	42	dep	immunostaining	1120:1133	arg1	[SOST					1174:1178	SCLEROSTIN [SOST	1163:1178	SCLEROSTIN [SOST	1163:1178	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	43	with	osteogenesis	1206:1217	arg1	differentiation					1233:1247	terminal differentiation	1224:1247	terminal differentiation	1224:1247	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	8	44	theme	architecture	1688:1699	arg1	combination					1663:1673	The clinically conformant combination	1637:1673	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration	1637:1825	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	4	45	theme	bone	656:659	arg1	BM-MSCs					682:688	BM-MSCs	682:688	BM-MSCs	682:688	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	4	45	theme	bone	656:659	arg1	MSCs					676:679	human bone marrow-derived MSCs	650:679	human bone marrow-derived MSCs (BM-MSCs)	650:689	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	8	46	theme	pore	1706:1709	arg1	sizes					1711:1715	pore sizes	1706:1715	pore sizes varying in the range of 20 to 200 μm	1706:1752	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	5	47	theme	BM-MSC-laden	1252:1263	arg1	composites					1276:1285	BM-MSC-laden CHT/HA/PCL composites	1252:1285	BM-MSC-laden CHT/HA/PCL composites	1252:1285	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	0	48	theme	porous	71:76	arg1	analogs					98:104	highly porous chitosan-based bone analogs	64:104	highly porous chitosan-based bone analogs	64:104	Tissue-specific mesenchymal stem cell-dependent osteogenesis in highly porous chitosan-based bone analogs.
33049125	5	49	theme	pronounced	1195:1204	arg1	osteogenesis					1206:1217	pronounced osteogenesis	1195:1217	pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites	1195:1285	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	4	50	theme	tissue	634:639	arg1	sources					641:647	three tissue sources	628:647	three tissue sources	628:647	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	6	51	theme	cell	1306:1309	arg1	functionality					1311:1323	The enhanced cell functionality	1293:1323	The enhanced cell functionality on CHT/HA/PCL composites	1293:1348	The enhanced cell functionality on CHT/HA/PCL composites was explained in terms of interplay among the surface properties and the optimal source of MSCs.
33049125	2	52	theme	early	349:353	arg1	osseointegration					355:370	early osseointegration	349:370	early osseointegration of highly porous scaffolds	349:397	However, the understanding of the stem cell progenitor-dependent cell functionality modulation and quantitative analysis of early osseointegration of highly porous scaffolds have not been explored.
33049125	8	53	theme	3D	1678:1679	arg1	architecture					1688:1699	3D porous architecture	1678:1699	3D porous architecture	1678:1699	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	1	54	theme	polymeric	205:213	arg1	implants					215:222	polymeric implants	205:222	polymeric implants	205:222	Among conventional fabrication techniques, freeze-drying process has widely been investigated for polymeric implants.
33049125	7	55	theme	rat	1476:1478	arg1	model					1487:1491	rat tibial model	1476:1491	rat tibial model	1476:1491	In addition, osteogenesis in rat tibial model over 6 weeks confirmed a better ratio of bone volume to the total volume for BM-MSC-laden composites over scaffold-only and defect-only groups.
33049125	4	56	link	jelly-derived	738:750	arg1	MSCs					752:755	Wharton's jelly-derived MSCs	728:755	Wharton's jelly-derived MSCs (WJ-MSCs)	728:765	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	4	56	link	jelly-derived	738:750	arg1	WJ-MSCs					758:764	WJ-MSCs	758:764	WJ-MSCs	758:764	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	2	57	theme	porous	382:387	arg1	scaffolds					389:397	highly porous scaffolds	375:397	highly porous scaffolds	375:397	However, the understanding of the stem cell progenitor-dependent cell functionality modulation and quantitative analysis of early osseointegration of highly porous scaffolds have not been explored.
33049125	6	58	theme	interplay	1376:1384	arg1	terms					1367:1371	terms	1367:1371	terms of interplay among the surface properties and the optimal source of MSCs	1367:1444	The enhanced cell functionality on CHT/HA/PCL composites was explained in terms of interplay among the surface properties and the optimal source of MSCs.
33049125	8	59	theme	μm	1751:1752	arg1	range					1732:1736	the range	1728:1736	the range of 20 to 200 μm	1728:1752	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	2	60	theme	progenitor-dependent	269:288	arg1	modulation					309:318	the stem cell progenitor-dependent cell functionality modulation	255:318	the stem cell progenitor-dependent cell functionality modulation	255:318	However, the understanding of the stem cell progenitor-dependent cell functionality modulation and quantitative analysis of early osseointegration of highly porous scaffolds have not been explored.
33049125	2	61	theme	stem	259:262	arg1	modulation					309:318	the stem cell progenitor-dependent cell functionality modulation	255:318	the stem cell progenitor-dependent cell functionality modulation	255:318	However, the understanding of the stem cell progenitor-dependent cell functionality modulation and quantitative analysis of early osseointegration of highly porous scaffolds have not been explored.
33049125	8	62	theme	in	1779:1780	arg1	degradation					1788:1798	controlled in vitro degradation	1768:1798	controlled in vitro degradation	1768:1798	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	5	63	theme	gene	984:987	arg1	expression					989:998	real-time gene expression	974:998	real-time gene expression (alkaline phosphatase [ALP]	974:1026	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	63	theme	gene	984:987	arg1	[ALP					1022:1025	alkaline phosphatase [ALP	1001:1025	alkaline phosphatase [ALP	1001:1025	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	63	theme	gene	984:987	arg1	microscopy					962:971	scanning electron microscopy	944:971	scanning electron microscopy	944:971	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	6	64	theme	MSCs	1441:1444	arg1	properties					1404:1413	the surface properties	1392:1413	the surface properties	1392:1413	The enhanced cell functionality on CHT/HA/PCL composites was explained in terms of interplay among the surface properties and the optimal source of MSCs.
33049125	6	64	theme	MSCs	1441:1444	arg1	source					1431:1436	the optimal source	1419:1436	the optimal source of MSCs	1419:1444	The enhanced cell functionality on CHT/HA/PCL composites was explained in terms of interplay among the surface properties and the optimal source of MSCs.
33049125	4	65	theme	jelly-derived	738:750	arg1	MSCs					752:755	Wharton's jelly-derived MSCs	728:755	Wharton's jelly-derived MSCs (WJ-MSCs)	728:765	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	4	65	theme	jelly-derived	738:750	arg1	WJ-MSCs					758:764	WJ-MSCs	758:764	WJ-MSCs	758:764	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	7	66	theme	defect-only	1617:1627	arg1	groups					1629:1634	scaffold-only and defect-only groups	1599:1634	scaffold-only and defect-only groups	1599:1634	In addition, osteogenesis in rat tibial model over 6 weeks confirmed a better ratio of bone volume to the total volume for BM-MSC-laden composites over scaffold-only and defect-only groups.
33049125	0	67	theme	bone	93:96	arg1	analogs					98:104	highly porous chitosan-based bone analogs	64:104	highly porous chitosan-based bone analogs	64:104	Tissue-specific mesenchymal stem cell-dependent osteogenesis in highly porous chitosan-based bone analogs.
33049125	2	68	theme	functionality	295:307	arg1	modulation					309:318	the stem cell progenitor-dependent cell functionality modulation	255:318	the stem cell progenitor-dependent cell functionality modulation	255:318	However, the understanding of the stem cell progenitor-dependent cell functionality modulation and quantitative analysis of early osseointegration of highly porous scaffolds have not been explored.
33049125	8	69	theme	early	1804:1808	arg1	osseointegration					1810:1825	early osseointegration	1804:1825	early osseointegration	1804:1825	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	5	70	theme	electron	953:960	arg1	expression					989:998	real-time gene expression	974:998	real-time gene expression (alkaline phosphatase [ALP]	974:1026	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	70	theme	electron	953:960	arg1	microscopy					962:971	scanning electron microscopy	944:971	scanning electron microscopy	944:971	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	7	71	theme	scaffold-only	1599:1611	arg1	groups					1629:1634	scaffold-only and defect-only groups	1599:1634	scaffold-only and defect-only groups	1599:1634	In addition, osteogenesis in rat tibial model over 6 weeks confirmed a better ratio of bone volume to the total volume for BM-MSC-laden composites over scaffold-only and defect-only groups.
33049125	5	72	theme	metabolic	797:805	arg1	assay					880:884	2,5 diphenyl-2H-tetrazoliumbromide) assay	844:884	2,5 diphenyl-2H-tetrazoliumbromide) assay	844:884	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	72	theme	metabolic	797:805	arg1	[3-4,5-dimethylthiazol-2yl-					816:842	metabolic activity [3-4,5-dimethylthiazol-2yl-	797:842	metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay]	797:885	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	8	73	with	combination	1663:1673	arg1	sizes					1711:1715	pore sizes	1706:1715	pore sizes varying in the range of 20 to 200 μm	1706:1752	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	4	74	theme	in	550:551	arg1	studies					559:565	The in vitro studies	546:565	The in vitro studies	546:565	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	3	75	dep	novel	444:448	arg1	multimaterial					466:478	multimaterial	466:478	multimaterial	466:478	Here, we developed a novel, highly porous, multimaterial composite, chitosan/hydroxyapatite/polycaprolactone (CHT/HA/PCL).
33049125	3	75	dep	novel	444:448	arg1	porous					458:463	porous	458:463	porous	458:463	Here, we developed a novel, highly porous, multimaterial composite, chitosan/hydroxyapatite/polycaprolactone (CHT/HA/PCL).
33049125	8	76	dep	together	1754:1761	arg1	with					1763:1766	with	1763:1766	with	1763:1766	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	4	77	from	sources	641:647	arg1	cells					610:614	mesenchymal stem cells	593:614	mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs)	593:765	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	4	77	from	sources	641:647	arg1	MSCs					617:620	MSCs	617:620	MSCs	617:620	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	8	78	theme	CHT/HA/PCL	1854:1863	arg1	composite					1865:1873	CHT/HA/PCL composite	1854:1873	CHT/HA/PCL composite	1854:1873	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	8	79	dep	200	1747:1749	arg1	to					1744:1745	to	1744:1745	to	1744:1745	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	5	80	theme	COL	1136:1138	arg1	I					1140:1140	COL I	1136:1140	COL I	1136:1140	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	81	theme	phosphatase	1010:1020	arg1	expression					989:998	real-time gene expression	974:998	real-time gene expression (alkaline phosphatase [ALP]	974:1026	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	81	theme	phosphatase	1010:1020	arg1	[ALP					1022:1025	alkaline phosphatase [ALP	1001:1025	alkaline phosphatase [ALP	1001:1025	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	3	82	theme	novel	444:448	arg1	chitosan/hydroxyapatite/polycaprolactone					491:530	chitosan/hydroxyapatite/polycaprolactone	491:530	chitosan/hydroxyapatite/polycaprolactone (CHT/HA/PCL)	491:543	Here, we developed a novel, highly porous, multimaterial composite, chitosan/hydroxyapatite/polycaprolactone (CHT/HA/PCL).
33049125	3	82	theme	novel	444:448	arg1	composite					480:488	a novel, highly porous, multimaterial composite	442:488	a novel, highly porous, multimaterial composite	442:488	Here, we developed a novel, highly porous, multimaterial composite, chitosan/hydroxyapatite/polycaprolactone (CHT/HA/PCL).
33049125	0	83	theme	cell-dependent	33:46	arg1	osteogenesis					48:59	cell-dependent osteogenesis	33:59	cell-dependent osteogenesis in highly porous chitosan-based bone analogs	33:104	Tissue-specific mesenchymal stem cell-dependent osteogenesis in highly porous chitosan-based bone analogs.
33049125	8	84	theme	potential	1880:1888	arg1	potential					1841:1849	the potential	1837:1849	the potential of CHT/HA/PCL composite	1837:1873	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	8	84	theme	potential	1880:1888	arg1	analog					1906:1911	a potential cancellous bone analog	1878:1911	a potential cancellous bone analog	1878:1911	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	1	85	theme	fabrication	126:136	arg1	techniques					138:147	conventional fabrication techniques	113:147	conventional fabrication techniques	113:147	Among conventional fabrication techniques, freeze-drying process has widely been investigated for polymeric implants.
33049125	4	86	theme	stem	605:608	arg1	cells					610:614	mesenchymal stem cells	593:614	mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs)	593:765	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	4	86	theme	stem	605:608	arg1	MSCs					617:620	MSCs	617:620	MSCs	617:620	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	5	87	theme	2,5	844:846	arg1	assay					880:884	2,5 diphenyl-2H-tetrazoliumbromide) assay	844:884	2,5 diphenyl-2H-tetrazoliumbromide) assay	844:884	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	87	theme	2,5	844:846	arg1	[3-4,5-dimethylthiazol-2yl-					816:842	metabolic activity [3-4,5-dimethylthiazol-2yl-	797:842	metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay]	797:885	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	5	88	theme	SCLEROSTIN	1163:1172	arg1	[SOST					1174:1178	SCLEROSTIN [SOST	1163:1178	SCLEROSTIN [SOST	1163:1178	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	7	89	theme	volume	1539:1544	arg1	ratio					1525:1529	a better ratio	1516:1529	a better ratio of bone volume to the total volume for BM-MSC-laden composites over scaffold-only and defect-only groups	1516:1634	In addition, osteogenesis in rat tibial model over 6 weeks confirmed a better ratio of bone volume to the total volume for BM-MSC-laden composites over scaffold-only and defect-only groups.
33049125	4	90	theme	marrow-derived	661:674	arg1	BM-MSCs					682:688	BM-MSCs	682:688	BM-MSCs	682:688	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	4	90	theme	marrow-derived	661:674	arg1	MSCs					676:679	human bone marrow-derived MSCs	650:679	human bone marrow-derived MSCs (BM-MSCs)	650:689	The in vitro studies have been performed using mesenchymal stem cells (MSCs) from three tissue sources: human bone marrow-derived MSCs (BM-MSCs), adipose-derived MSCs (AD-MSCs), and Wharton's jelly-derived MSCs (WJ-MSCs).
33049125	2	91	theme	scaffolds	389:397	arg1	osseointegration					355:370	early osseointegration	349:370	early osseointegration of highly porous scaffolds	349:397	However, the understanding of the stem cell progenitor-dependent cell functionality modulation and quantitative analysis of early osseointegration of highly porous scaffolds have not been explored.
33049125	8	92	theme	conformant	1652:1661	arg1	combination					1663:1673	The clinically conformant combination	1637:1673	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration	1637:1825	The clinically conformant combination of 3D porous architecture with pore sizes varying in the range of 20 to 200 μm together with controlled in vitro degradation and early osseointegration establish the potential of CHT/HA/PCL composite as a potential cancellous bone analog.
33049125	5	93	theme	CHT/HA/PCL	1265:1274	arg1	composites					1276:1285	BM-MSC-laden CHT/HA/PCL composites	1252:1285	BM-MSC-laden CHT/HA/PCL composites	1252:1285	Although cell attachment and metabolic activity [3-4,5-dimethylthiazol-2yl-(2,5 diphenyl-2H-tetrazoliumbromide) assay] were ore enhanced in WJ-MSC-laden CHT/HA/PCL composites, scanning electron microscopy, real-time gene expression (alkaline phosphatase [ALP], collagen type I [Col I], osteocalcin [OCN], and bone morphogenetic protein 4 [BMP-4]), and immunostaining (COL I, β-CATENIN, OCN, and SCLEROSTIN [SOST]) demonstrated pronounced osteogenesis with terminal differentiation on BM-MSC-laden CHT/HA/PCL composites only.
33049125	6	94	from	functionality	1311:1323	arg1	composites					1339:1348	CHT/HA/PCL composites	1328:1348	CHT/HA/PCL composites	1328:1348	The enhanced cell functionality on CHT/HA/PCL composites was explained in terms of interplay among the surface properties and the optimal source of MSCs.
32105684	0	0	theme	ultrafiltration	86:100	arg1	acids					68:72	humic acids	62:72	humic acids removal and ultrafiltration membrane fouling control	62:125	Enteromorpha prolifera polysaccharide based coagulant aid for humic acids removal and ultrafiltration membrane fouling control.
32105684	0	0	theme	ultrafiltration	86:100	arg1	control					119:125	ultrafiltration membrane fouling control	86:125	ultrafiltration membrane fouling control	86:125	Enteromorpha prolifera polysaccharide based coagulant aid for humic acids removal and ultrafiltration membrane fouling control.
32105684	4	1	theme	removal	572:578	arg1	terms					554:558	terms	554:558	terms of organics removal, floc properties and membrane fouling degree	554:623	Its coagulant aid efficiency and mechanism were investigated in terms of organics removal, floc properties and membrane fouling degree.
32105684	7	2	theme	recovery	946:953	arg1	factor					955:960	recovery factor	946:960	recovery factor	946:960	Due to the bridging-sweep aid role of Ep, flocs sizes, growth rate and recovery factor reached 470 μm, 62.6 μm/min and 0.492, respectively, while only 170 μm, 14.0 μm/min and 0.326 were obtained by PAM.
32105684	4	3	theme	floc	581:584	arg1	properties					586:595	floc properties	581:595	floc properties	581:595	Its coagulant aid efficiency and mechanism were investigated in terms of organics removal, floc properties and membrane fouling degree.
32105684	4	4	theme	coagulant	494:502	arg1	efficiency					508:517	Its coagulant aid efficiency	490:517	Its coagulant aid efficiency	490:517	Its coagulant aid efficiency and mechanism were investigated in terms of organics removal, floc properties and membrane fouling degree.
32105684	7	5	theme	growth	930:935	arg1	rate					937:940	growth rate	930:940	growth rate	930:940	Due to the bridging-sweep aid role of Ep, flocs sizes, growth rate and recovery factor reached 470 μm, 62.6 μm/min and 0.492, respectively, while only 170 μm, 14.0 μm/min and 0.326 were obtained by PAM.
32105684	2	6	theme	biological	323:332	arg1	safety					334:339	high biological safety	318:339	high biological safety	318:339	Developing new coagulant aid with high biological safety is urgently demanded.
32105684	9	7	theme	higher	1348:1353	arg1	removal					1364:1370	higher organics removal	1348:1370	higher organics removal	1348:1370	As a result, Ep could be considered as a potential substitute of PAM, since better biosecurity, higher organics removal and lower membrane fouling could be obtained simultaneously by Ep addition.
32105684	0	8	theme	fouling	111:117	arg1	acids					68:72	humic acids	62:72	humic acids removal and ultrafiltration membrane fouling control	62:125	Enteromorpha prolifera polysaccharide based coagulant aid for humic acids removal and ultrafiltration membrane fouling control.
32105684	0	8	theme	fouling	111:117	arg1	control					119:125	ultrafiltration membrane fouling control	86:125	ultrafiltration membrane fouling control	86:125	Enteromorpha prolifera polysaccharide based coagulant aid for humic acids removal and ultrafiltration membrane fouling control.
32105684	6	9	used	used	822:825	arg2	Ep					815:816	0.3 mg/L Ep	806:816	0.3 mg/L Ep	806:816	Results showed that organics removal could be increased by 23% when 0.3 mg/L Ep was used, which exhibited comparable aid effects to PAM.
32105684	9	10	theme	lower	1376:1380	arg1	fouling					1391:1397	lower membrane fouling	1376:1397	lower membrane fouling	1376:1397	As a result, Ep could be considered as a potential substitute of PAM, since better biosecurity, higher organics removal and lower membrane fouling could be obtained simultaneously by Ep addition.
32105684	7	11	dep	flocs	917:921	arg1	sizes					923:927	sizes	923:927	sizes	923:927	Due to the bridging-sweep aid role of Ep, flocs sizes, growth rate and recovery factor reached 470 μm, 62.6 μm/min and 0.492, respectively, while only 170 μm, 14.0 μm/min and 0.326 were obtained by PAM.
32105684	8	12	theme	porous	1113:1118	arg1	structures					1139:1148	more porous and multi-branched structures	1108:1148	more porous and multi-branched structures	1108:1148	Additionally, flocs exhibited more porous and multi-branched structures when Ep was applied, which caused less ultrafiltration membrane fouling (eventual J/J0 value = 0.52).
32105684	0	13	theme	membrane	102:109	arg1	acids					68:72	humic acids	62:72	humic acids removal and ultrafiltration membrane fouling control	62:125	Enteromorpha prolifera polysaccharide based coagulant aid for humic acids removal and ultrafiltration membrane fouling control.
32105684	0	13	theme	membrane	102:109	arg1	control					119:125	ultrafiltration membrane fouling control	86:125	ultrafiltration membrane fouling control	86:125	Enteromorpha prolifera polysaccharide based coagulant aid for humic acids removal and ultrafiltration membrane fouling control.
32105684	6	14	theme	0.3 mg/L	806:813	arg1	Ep					815:816	0.3 mg/L Ep	806:816	0.3 mg/L Ep	806:816	Results showed that organics removal could be increased by 23% when 0.3 mg/L Ep was used, which exhibited comparable aid effects to PAM.
32105684	1	15	used	used	158:161	arg2	Polyacrylamide					128:141	Polyacrylamide	128:141	Polyacrylamide (PAM)	128:147	Polyacrylamide (PAM) has been used as a coagulant aid in water treatment process for past decades, but it has caused great damages to human nervous system.
32105684	1	15	used	used	158:161	arg2	coagulant					168:176	coagulant	168:176	coagulant	168:176	Polyacrylamide (PAM) has been used as a coagulant aid in water treatment process for past decades, but it has caused great damages to human nervous system.
32105684	1	15	used	used	158:161	arg2	PAM					144:146	PAM	144:146	PAM	144:146	Polyacrylamide (PAM) has been used as a coagulant aid in water treatment process for past decades, but it has caused great damages to human nervous system.
32105684	1	16	theme	great	245:249	arg1	damages					251:257	great damages	245:257	great damages to human nervous system	245:281	Polyacrylamide (PAM) has been used as a coagulant aid in water treatment process for past decades, but it has caused great damages to human nervous system.
32105684	8	17	theme	multi-branched	1124:1137	arg1	structures					1139:1148	more porous and multi-branched structures	1108:1148	more porous and multi-branched structures	1108:1148	Additionally, flocs exhibited more porous and multi-branched structures when Ep was applied, which caused less ultrafiltration membrane fouling (eventual J/J0 value = 0.52).
32105684	8	18	theme	ultrafiltration	1189:1203	arg1	fouling					1214:1220	less ultrafiltration membrane fouling	1184:1220	less ultrafiltration membrane fouling (eventual J/J0 value = 0.52)	1184:1249	Additionally, flocs exhibited more porous and multi-branched structures when Ep was applied, which caused less ultrafiltration membrane fouling (eventual J/J0 value = 0.52).
32105684	8	18	theme	ultrafiltration	1189:1203	arg1	value = 0.52					1237:1248	eventual J/J0 value = 0.52	1223:1248	eventual J/J0 value = 0.52	1223:1248	Additionally, flocs exhibited more porous and multi-branched structures when Ep was applied, which caused less ultrafiltration membrane fouling (eventual J/J0 value = 0.52).
32105684	4	19	theme	properties	586:595	arg1	terms					554:558	terms	554:558	terms of organics removal, floc properties and membrane fouling degree	554:623	Its coagulant aid efficiency and mechanism were investigated in terms of organics removal, floc properties and membrane fouling degree.
32105684	9	20	dep	potential	1293:1301	arg1	substitute					1303:1312	substitute	1303:1312	substitute	1303:1312	As a result, Ep could be considered as a potential substitute of PAM, since better biosecurity, higher organics removal and lower membrane fouling could be obtained simultaneously by Ep addition.
32105684	3	21	theme	good	429:432	arg1	Ep					485:486	Ep	485:486	Ep	485:486	This study provides a natural biomacromolecule coagulant aid with good biosecurity-Enteromorpha prolifera polysaccharide (Ep).
32105684	3	21	theme	good	429:432	arg1	polysaccharide					469:482	good biosecurity-Enteromorpha prolifera polysaccharide	429:482	good biosecurity-Enteromorpha prolifera polysaccharide (Ep)	429:487	This study provides a natural biomacromolecule coagulant aid with good biosecurity-Enteromorpha prolifera polysaccharide (Ep).
32105684	0	22	theme	prolifera	13:21	arg1	polysaccharide					23:36	Enteromorpha prolifera polysaccharide	0:36	Enteromorpha prolifera polysaccharide	0:36	Enteromorpha prolifera polysaccharide based coagulant aid for humic acids removal and ultrafiltration membrane fouling control.
32105684	4	23	theme	organics	563:570	arg1	removal					572:578	organics removal	563:578	organics removal	563:578	Its coagulant aid efficiency and mechanism were investigated in terms of organics removal, floc properties and membrane fouling degree.
32105684	7	24	theme	Ep	913:914	arg1	role					905:908	the bridging-sweep aid role	882:908	the bridging-sweep aid role of Ep	882:914	Due to the bridging-sweep aid role of Ep, flocs sizes, growth rate and recovery factor reached 470 μm, 62.6 μm/min and 0.492, respectively, while only 170 μm, 14.0 μm/min and 0.326 were obtained by PAM.
32105684	2	25	theme	new	295:297	arg1	coagulant					299:307	Developing new coagulant aid	284:311	Developing new coagulant aid with high biological safety	284:339	Developing new coagulant aid with high biological safety is urgently demanded.
32105684	9	26	theme	membrane	1382:1389	arg1	fouling					1391:1397	lower membrane fouling	1376:1397	lower membrane fouling	1376:1397	As a result, Ep could be considered as a potential substitute of PAM, since better biosecurity, higher organics removal and lower membrane fouling could be obtained simultaneously by Ep addition.
32105684	0	27	theme	Enteromorpha	0:11	arg1	polysaccharide					23:36	Enteromorpha prolifera polysaccharide	0:36	Enteromorpha prolifera polysaccharide	0:36	Enteromorpha prolifera polysaccharide based coagulant aid for humic acids removal and ultrafiltration membrane fouling control.
32105684	5	28	theme	contrast	639:646	arg1	experiments					648:658	contrast experiments	639:658	contrast experiments	639:658	In addition, contrast experiments were conducted with PAM to evaluate its potential of industrial applications.
32105684	2	29	theme	Developing	284:293	arg1	coagulant					299:307	Developing new coagulant aid	284:311	Developing new coagulant aid with high biological safety	284:339	Developing new coagulant aid with high biological safety is urgently demanded.
32105684	8	30	theme	membrane	1205:1212	arg1	fouling					1214:1220	less ultrafiltration membrane fouling	1184:1220	less ultrafiltration membrane fouling (eventual J/J0 value = 0.52)	1184:1249	Additionally, flocs exhibited more porous and multi-branched structures when Ep was applied, which caused less ultrafiltration membrane fouling (eventual J/J0 value = 0.52).
32105684	8	30	theme	membrane	1205:1212	arg1	value = 0.52					1237:1248	eventual J/J0 value = 0.52	1223:1248	eventual J/J0 value = 0.52	1223:1248	Additionally, flocs exhibited more porous and multi-branched structures when Ep was applied, which caused less ultrafiltration membrane fouling (eventual J/J0 value = 0.52).
32105684	0	31	dep	coagulant	44:52	arg1	aid					54:56	aid	54:56	aid	54:56	Enteromorpha prolifera polysaccharide based coagulant aid for humic acids removal and ultrafiltration membrane fouling control.
32105684	9	32	theme	PAM	1317:1319	arg1	Ep					1265:1266	Ep	1265:1266	Ep	1265:1266	As a result, Ep could be considered as a potential substitute of PAM, since better biosecurity, higher organics removal and lower membrane fouling could be obtained simultaneously by Ep addition.
32105684	9	32	theme	PAM	1317:1319	arg1	potential					1293:1301	a potential substitute	1291:1312	a potential substitute of PAM	1291:1319	As a result, Ep could be considered as a potential substitute of PAM, since better biosecurity, higher organics removal and lower membrane fouling could be obtained simultaneously by Ep addition.
32105684	1	33	theme	water	185:189	arg1	process					201:207	water treatment process	185:207	water treatment process	185:207	Polyacrylamide (PAM) has been used as a coagulant aid in water treatment process for past decades, but it has caused great damages to human nervous system.
32105684	2	34	dep	coagulant	299:307	arg1	aid					309:311	aid	309:311	aid	309:311	Developing new coagulant aid with high biological safety is urgently demanded.
32105684	1	35	theme	human	262:266	arg1	system					276:281	human nervous system	262:281	human nervous system	262:281	Polyacrylamide (PAM) has been used as a coagulant aid in water treatment process for past decades, but it has caused great damages to human nervous system.
32105684	0	36	dep	acids	68:72	arg1	acids					68:72	humic acids	62:72	humic acids removal and ultrafiltration membrane fouling control	62:125	Enteromorpha prolifera polysaccharide based coagulant aid for humic acids removal and ultrafiltration membrane fouling control.
32105684	0	36	dep	acids	68:72	arg1	control					119:125	ultrafiltration membrane fouling control	86:125	ultrafiltration membrane fouling control	86:125	Enteromorpha prolifera polysaccharide based coagulant aid for humic acids removal and ultrafiltration membrane fouling control.
32105684	0	36	dep	acids	68:72	arg1	removal					74:80	removal	74:80	removal	74:80	Enteromorpha prolifera polysaccharide based coagulant aid for humic acids removal and ultrafiltration membrane fouling control.
32105684	1	37	theme	treatment	191:199	arg1	process					201:207	water treatment process	185:207	water treatment process	185:207	Polyacrylamide (PAM) has been used as a coagulant aid in water treatment process for past decades, but it has caused great damages to human nervous system.
32105684	3	38	theme	natural	385:391	arg1	coagulant					410:418	a natural biomacromolecule coagulant aid	383:422	a natural biomacromolecule coagulant aid with good biosecurity-Enteromorpha prolifera polysaccharide (Ep)	383:487	This study provides a natural biomacromolecule coagulant aid with good biosecurity-Enteromorpha prolifera polysaccharide (Ep).
32105684	1	39	theme	nervous	268:274	arg1	system					276:281	human nervous system	262:281	human nervous system	262:281	Polyacrylamide (PAM) has been used as a coagulant aid in water treatment process for past decades, but it has caused great damages to human nervous system.
32105684	5	40	theme	industrial	713:722	arg1	applications					724:735	industrial applications	713:735	industrial applications	713:735	In addition, contrast experiments were conducted with PAM to evaluate its potential of industrial applications.
32105684	2	41	with	coagulant	299:307	arg1	safety					334:339	high biological safety	318:339	high biological safety	318:339	Developing new coagulant aid with high biological safety is urgently demanded.
32105684	3	42	dep	coagulant	410:418	arg1	aid					420:422	aid	420:422	aid	420:422	This study provides a natural biomacromolecule coagulant aid with good biosecurity-Enteromorpha prolifera polysaccharide (Ep).
32105684	3	43	theme	biosecurity-Enteromorpha	434:457	arg1	Ep					485:486	Ep	485:486	Ep	485:486	This study provides a natural biomacromolecule coagulant aid with good biosecurity-Enteromorpha prolifera polysaccharide (Ep).
32105684	3	43	theme	biosecurity-Enteromorpha	434:457	arg1	polysaccharide					469:482	good biosecurity-Enteromorpha prolifera polysaccharide	429:482	good biosecurity-Enteromorpha prolifera polysaccharide (Ep)	429:487	This study provides a natural biomacromolecule coagulant aid with good biosecurity-Enteromorpha prolifera polysaccharide (Ep).
32105684	3	44	theme	biomacromolecule	393:408	arg1	coagulant					410:418	a natural biomacromolecule coagulant aid	383:422	a natural biomacromolecule coagulant aid with good biosecurity-Enteromorpha prolifera polysaccharide (Ep)	383:487	This study provides a natural biomacromolecule coagulant aid with good biosecurity-Enteromorpha prolifera polysaccharide (Ep).
32105684	5	45	theme	applications	724:735	arg1	potential					700:708	its potential	696:708	its potential of industrial applications	696:735	In addition, contrast experiments were conducted with PAM to evaluate its potential of industrial applications.
32105684	3	46	theme	prolifera	459:467	arg1	Ep					485:486	Ep	485:486	Ep	485:486	This study provides a natural biomacromolecule coagulant aid with good biosecurity-Enteromorpha prolifera polysaccharide (Ep).
32105684	3	46	theme	prolifera	459:467	arg1	polysaccharide					469:482	good biosecurity-Enteromorpha prolifera polysaccharide	429:482	good biosecurity-Enteromorpha prolifera polysaccharide (Ep)	429:487	This study provides a natural biomacromolecule coagulant aid with good biosecurity-Enteromorpha prolifera polysaccharide (Ep).
32105684	0	47	theme	humic	62:66	arg1	acids					68:72	humic acids	62:72	humic acids removal and ultrafiltration membrane fouling control	62:125	Enteromorpha prolifera polysaccharide based coagulant aid for humic acids removal and ultrafiltration membrane fouling control.
32105684	0	47	theme	humic	62:66	arg1	control					119:125	ultrafiltration membrane fouling control	86:125	ultrafiltration membrane fouling control	86:125	Enteromorpha prolifera polysaccharide based coagulant aid for humic acids removal and ultrafiltration membrane fouling control.
32105684	0	47	theme	humic	62:66	arg1	removal					74:80	removal	74:80	removal	74:80	Enteromorpha prolifera polysaccharide based coagulant aid for humic acids removal and ultrafiltration membrane fouling control.
32105684	4	48	dep	efficiency	508:517	arg1	aid					504:506	aid	504:506	aid	504:506	Its coagulant aid efficiency and mechanism were investigated in terms of organics removal, floc properties and membrane fouling degree.
32105684	3	49	with	coagulant	410:418	arg1	Ep					485:486	Ep	485:486	Ep	485:486	This study provides a natural biomacromolecule coagulant aid with good biosecurity-Enteromorpha prolifera polysaccharide (Ep).
32105684	3	49	with	coagulant	410:418	arg1	polysaccharide					469:482	good biosecurity-Enteromorpha prolifera polysaccharide	429:482	good biosecurity-Enteromorpha prolifera polysaccharide (Ep)	429:487	This study provides a natural biomacromolecule coagulant aid with good biosecurity-Enteromorpha prolifera polysaccharide (Ep).
32105684	6	50	theme	comparable	844:853	arg1	effects					859:865	comparable aid effects	844:865	comparable aid effects	844:865	Results showed that organics removal could be increased by 23% when 0.3 mg/L Ep was used, which exhibited comparable aid effects to PAM.
32105684	2	51	theme	high	318:321	arg1	safety					334:339	high biological safety	318:339	high biological safety	318:339	Developing new coagulant aid with high biological safety is urgently demanded.
32105684	1	52	dep	coagulant	168:176	arg1	aid					178:180	aid	178:180	aid in water treatment process for past decades	178:224	Polyacrylamide (PAM) has been used as a coagulant aid in water treatment process for past decades, but it has caused great damages to human nervous system.
32105684	9	53	theme	Ep	1435:1436	arg1	addition					1438:1445	Ep addition	1435:1445	Ep addition	1435:1445	As a result, Ep could be considered as a potential substitute of PAM, since better biosecurity, higher organics removal and lower membrane fouling could be obtained simultaneously by Ep addition.
32105684	7	54	theme	aid	901:903	arg1	role					905:908	the bridging-sweep aid role	882:908	the bridging-sweep aid role of Ep	882:914	Due to the bridging-sweep aid role of Ep, flocs sizes, growth rate and recovery factor reached 470 μm, 62.6 μm/min and 0.492, respectively, while only 170 μm, 14.0 μm/min and 0.326 were obtained by PAM.
32105684	6	55	dep	effects	859:865	arg1	aid					855:857	aid	855:857	aid	855:857	Results showed that organics removal could be increased by 23% when 0.3 mg/L Ep was used, which exhibited comparable aid effects to PAM.
32105684	6	56	theme	organics	758:765	arg1	removal					767:773	organics removal	758:773	organics removal	758:773	Results showed that organics removal could be increased by 23% when 0.3 mg/L Ep was used, which exhibited comparable aid effects to PAM.
32105684	1	57	theme	past	213:216	arg1	decades					218:224	past decades	213:224	past decades	213:224	Polyacrylamide (PAM) has been used as a coagulant aid in water treatment process for past decades, but it has caused great damages to human nervous system.
32105684	4	58	theme	membrane	601:608	arg1	degree					618:623	membrane fouling degree	601:623	membrane fouling degree	601:623	Its coagulant aid efficiency and mechanism were investigated in terms of organics removal, floc properties and membrane fouling degree.
32105684	8	59	theme	J/J0	1232:1235	arg1	fouling					1214:1220	less ultrafiltration membrane fouling	1184:1220	less ultrafiltration membrane fouling (eventual J/J0 value = 0.52)	1184:1249	Additionally, flocs exhibited more porous and multi-branched structures when Ep was applied, which caused less ultrafiltration membrane fouling (eventual J/J0 value = 0.52).
32105684	8	59	theme	J/J0	1232:1235	arg1	value = 0.52					1237:1248	eventual J/J0 value = 0.52	1223:1248	eventual J/J0 value = 0.52	1223:1248	Additionally, flocs exhibited more porous and multi-branched structures when Ep was applied, which caused less ultrafiltration membrane fouling (eventual J/J0 value = 0.52).
32105684	8	60	theme	eventual	1223:1230	arg1	fouling					1214:1220	less ultrafiltration membrane fouling	1184:1220	less ultrafiltration membrane fouling (eventual J/J0 value = 0.52)	1184:1249	Additionally, flocs exhibited more porous and multi-branched structures when Ep was applied, which caused less ultrafiltration membrane fouling (eventual J/J0 value = 0.52).
32105684	8	60	theme	eventual	1223:1230	arg1	value = 0.52					1237:1248	eventual J/J0 value = 0.52	1223:1248	eventual J/J0 value = 0.52	1223:1248	Additionally, flocs exhibited more porous and multi-branched structures when Ep was applied, which caused less ultrafiltration membrane fouling (eventual J/J0 value = 0.52).
32105684	7	61	theme	bridging-sweep	886:899	arg1	role					905:908	the bridging-sweep aid role	882:908	the bridging-sweep aid role of Ep	882:914	Due to the bridging-sweep aid role of Ep, flocs sizes, growth rate and recovery factor reached 470 μm, 62.6 μm/min and 0.492, respectively, while only 170 μm, 14.0 μm/min and 0.326 were obtained by PAM.
32105684	9	62	theme	better	1328:1333	arg1	biosecurity					1335:1345	better biosecurity	1328:1345	better biosecurity	1328:1345	As a result, Ep could be considered as a potential substitute of PAM, since better biosecurity, higher organics removal and lower membrane fouling could be obtained simultaneously by Ep addition.
32105684	9	63	theme	organics	1355:1362	arg1	removal					1364:1370	higher organics removal	1348:1370	higher organics removal	1348:1370	As a result, Ep could be considered as a potential substitute of PAM, since better biosecurity, higher organics removal and lower membrane fouling could be obtained simultaneously by Ep addition.
32105684	4	64	theme	fouling	610:616	arg1	degree					618:623	membrane fouling degree	601:623	membrane fouling degree	601:623	Its coagulant aid efficiency and mechanism were investigated in terms of organics removal, floc properties and membrane fouling degree.
32105684	4	65	theme	degree	618:623	arg1	terms					554:558	terms	554:558	terms of organics removal, floc properties and membrane fouling degree	554:623	Its coagulant aid efficiency and mechanism were investigated in terms of organics removal, floc properties and membrane fouling degree.
34072122	14	0	theme	single	2190:2195	arg1	test					2210:2213	the single flame source test	2186:2213	the single flame source test	2186:2213	In addition to cone calorimeter measurements, results from the single flame source test, limiting oxygen index determination and thermogravimetric analysis, are shown and discussed.
34072122	10	1	theme	fire	1594:1597	arg1	behavior					1599:1606	the fire behavior	1590:1606	the fire behavior	1590:1606	The influence of weathering on the fire behavior was small compared to the difference between fire-retarded and non-fire-retarded materials.
34072122	11	2	from	calorimeter	1722:1732	arg1	Results					1700:1706	Results	1700:1706	Results from the cone calorimeter	1700:1732	Results from the cone calorimeter were analyzed with regard to ETAG 028, which provides requirements related to the durability of fire performance of building products.
34072122	11	3	theme	ETAG	1763:1766	arg1	028					1768:1770	ETAG 028	1763:1770	ETAG 028	1763:1770	Results from the cone calorimeter were analyzed with regard to ETAG 028, which provides requirements related to the durability of fire performance of building products.
34072122	1	4	theme	fiber-reinforced	269:284	arg1	thermoplastics					286:299	natural fiber-reinforced thermoplastics	261:299	natural fiber-reinforced thermoplastics	261:299	At present, little information is available in the scientific literature related to the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics.
34072122	9	5	from	Use	1413:1415	arg1	layer					1447:1451	the co-extruded layer	1431:1451	the co-extruded layer	1431:1451	Use of the APP in the co-extruded layer worsened color change compared to the formulation without APP but the pHRR was not significantly changed.
34072122	4	6	theme	Various	691:697	arg1	FR					699:700	Various FR	691:700	Various FR for inclusion in the co-extruded layer	691:739	Various FR for inclusion in the co-extruded layer were screened based on UL-94 tests.
34072122	3	7	theme	hollow-core	585:595	arg1	profiles					597:604	either solid or hollow-core profiles	569:604	either solid or hollow-core profiles	569:604	Profile geometries were either solid or hollow-core profiles, and fire-retardants (FR) were added either in the co-extruded layer or in the bulk.
34072122	5	8	theme	ammonium	871:878	arg1	APP					895:897	APP	895:897	APP	895:897	For profile extrusion, two types of FR were chosen: a coated intumescent combination based on ammonium polyphosphate (APP) and an APP coated with melamine and without formaldehyde.
34072122	5	8	theme	ammonium	871:878	arg1	polyphosphate					880:892	ammonium polyphosphate	871:892	ammonium polyphosphate (APP)	871:898	For profile extrusion, two types of FR were chosen: a coated intumescent combination based on ammonium polyphosphate (APP) and an APP coated with melamine and without formaldehyde.
34072122	1	9	theme	thermoplastics	286:299	arg1	durability					200:209	the durability	196:209	the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics	196:299	At present, little information is available in the scientific literature related to the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics.
34072122	1	9	theme	thermoplastics	286:299	arg1	resistance					223:232	weathering resistance	212:232	weathering resistance	212:232	At present, little information is available in the scientific literature related to the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics.
34072122	7	10	theme	test	1290:1293	arg1	conditions					1295:1304	the test conditions	1286:1304	the test conditions	1286:1304	However, even before weathering, pHRR of the profiles was relatively high, with best (lowest) values between 230 and 250 kW/m2 under the test conditions.
34072122	14	11	theme	calorimeter	2147:2157	arg1	measurements					2159:2170	cone calorimeter measurements	2142:2170	cone calorimeter measurements	2142:2170	In addition to cone calorimeter measurements, results from the single flame source test, limiting oxygen index determination and thermogravimetric analysis, are shown and discussed.
34072122	2	12	theme	thermoplastic	316:328	arg1	profiles					330:337	thermoplastic profiles	316:337	thermoplastic profiles for façade applications based on high-density polyethylene, wheat straw particles, and fire-retardants	316:440	In this work, thermoplastic profiles for façade applications based on high-density polyethylene, wheat straw particles, and fire-retardants were extruded and their reaction-to-fire performance before and after artificial weathering evaluated.
34072122	15	13	theme	X-ray	2417:2421	arg1	data					2423:2426	energy dispersive X-ray data	2399:2426	energy dispersive X-ray data	2399:2426	Strength properties, water uptake and swelling of the profiles, thermal conductivity, and energy dispersive X-ray data are also presented.
34072122	6	14	dep	64	1126:1127	arg1	to					1123:1124	to	1123:1124	to	1123:1124	Before weathering, the peak heat release rate (pHRR) and the total heat release (THR), which were determined using cone calorimeter measurements, were reduced by up to 64% and 67% due to the FR.
34072122	15	15	theme	energy	2399:2404	arg1	data					2423:2426	energy dispersive X-ray data	2399:2426	energy dispersive X-ray data	2399:2426	Strength properties, water uptake and swelling of the profiles, thermal conductivity, and energy dispersive X-ray data are also presented.
34072122	5	16	theme	intumescent	838:848	arg1	combination					850:860	a coated intumescent combination	829:860	a coated intumescent combination based on ammonium polyphosphate (APP) and an APP coated with melamine and without formaldehyde	829:955	For profile extrusion, two types of FR were chosen: a coated intumescent combination based on ammonium polyphosphate (APP) and an APP coated with melamine and without formaldehyde.
34072122	7	17	with	high	1222:1225	arg1	values					1247:1252	best (lowest) values	1233:1252	best (lowest) values between 230 and 250 kW/m2	1233:1278	However, even before weathering, pHRR of the profiles was relatively high, with best (lowest) values between 230 and 250 kW/m2 under the test conditions.
34072122	11	18	theme	products	1859:1866	arg1	performance					1835:1845	fire performance	1830:1845	fire performance of building products	1830:1866	Results from the cone calorimeter were analyzed with regard to ETAG 028, which provides requirements related to the durability of fire performance of building products.
34072122	1	19	theme	weathering	212:221	arg1	durability					200:209	the durability	196:209	the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics	196:299	At present, little information is available in the scientific literature related to the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics.
34072122	1	19	theme	weathering	212:221	arg1	resistance					223:232	weathering resistance	212:232	weathering resistance	212:232	At present, little information is available in the scientific literature related to the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics.
34072122	8	20	theme	weathering	1335:1344	arg1	days					1316:1319	28 days	1313:1319	28 days of artificial weathering	1313:1344	After 28 days of artificial weathering, changes in reaction-to-fire performance and color were evaluated.
34072122	15	21	theme	Strength	2309:2316	arg1	properties					2318:2327	Strength properties	2309:2327	Strength properties	2309:2327	Strength properties, water uptake and swelling of the profiles, thermal conductivity, and energy dispersive X-ray data are also presented.
34072122	8	22	theme	reaction-to-fire	1358:1373	arg1	performance					1375:1385	reaction-to-fire performance	1358:1385	reaction-to-fire performance	1358:1385	After 28 days of artificial weathering, changes in reaction-to-fire performance and color were evaluated.
34072122	14	23	theme	thermogravimetric	2256:2272	arg1	analysis					2274:2281	thermogravimetric analysis	2256:2281	thermogravimetric analysis	2256:2281	In addition to cone calorimeter measurements, results from the single flame source test, limiting oxygen index determination and thermogravimetric analysis, are shown and discussed.
34072122	14	23	theme	thermogravimetric	2256:2272	arg1	results					2173:2179	results	2173:2179	results from the single flame source test	2173:2213	In addition to cone calorimeter measurements, results from the single flame source test, limiting oxygen index determination and thermogravimetric analysis, are shown and discussed.
34072122	5	24	theme	profile	781:787	arg1	extrusion					789:797	profile extrusion	781:797	profile extrusion	781:797	For profile extrusion, two types of FR were chosen: a coated intumescent combination based on ammonium polyphosphate (APP) and an APP coated with melamine and without formaldehyde.
34072122	4	25	theme	co-extruded	723:733	arg1	layer					735:739	the co-extruded layer	719:739	the co-extruded layer	719:739	Various FR for inclusion in the co-extruded layer were screened based on UL-94 tests.
34072122	6	26	theme	release	991:997	arg1	pHRR					1005:1008	pHRR	1005:1008	pHRR	1005:1008	Before weathering, the peak heat release rate (pHRR) and the total heat release (THR), which were determined using cone calorimeter measurements, were reduced by up to 64% and 67% due to the FR.
34072122	6	26	theme	release	991:997	arg1	rate					999:1002	the peak heat release rate	977:1002	the peak heat release rate (pHRR)	977:1009	Before weathering, the peak heat release rate (pHRR) and the total heat release (THR), which were determined using cone calorimeter measurements, were reduced by up to 64% and 67% due to the FR.
34072122	0	27	theme	Straw	96:100	arg1	Residues					102:109	Wheat Straw Residues	90:109	Wheat Straw Residues	90:109	Durability of Flame-Retarded, Co-Extruded Profiles Based on High-Density Polyethylene and Wheat Straw Residues.
34072122	15	28	theme	water	2330:2334	arg1	uptake					2336:2341	water uptake	2330:2341	water uptake	2330:2341	Strength properties, water uptake and swelling of the profiles, thermal conductivity, and energy dispersive X-ray data are also presented.
34072122	6	29	theme	peak	981:984	arg1	pHRR					1005:1008	pHRR	1005:1008	pHRR	1005:1008	Before weathering, the peak heat release rate (pHRR) and the total heat release (THR), which were determined using cone calorimeter measurements, were reduced by up to 64% and 67% due to the FR.
34072122	6	29	theme	peak	981:984	arg1	rate					999:1002	the peak heat release rate	977:1002	the peak heat release rate (pHRR)	977:1009	Before weathering, the peak heat release rate (pHRR) and the total heat release (THR), which were determined using cone calorimeter measurements, were reduced by up to 64% and 67% due to the FR.
34072122	14	30	dep	measurements	2159:2170	arg1	addition					2130:2137	addition	2130:2137	addition	2130:2137	In addition to cone calorimeter measurements, results from the single flame source test, limiting oxygen index determination and thermogravimetric analysis, are shown and discussed.
34072122	11	31	theme	related	1801:1807	arg1	requirements					1788:1799	requirements	1788:1799	requirements related to the durability of fire performance of building products	1788:1866	Results from the cone calorimeter were analyzed with regard to ETAG 028, which provides requirements related to the durability of fire performance of building products.
34072122	8	32	from	changes	1347:1353	arg1	color					1391:1395	color	1391:1395	color	1391:1395	After 28 days of artificial weathering, changes in reaction-to-fire performance and color were evaluated.
34072122	8	32	from	changes	1347:1353	arg1	performance					1375:1385	reaction-to-fire performance	1358:1385	reaction-to-fire performance	1358:1385	After 28 days of artificial weathering, changes in reaction-to-fire performance and color were evaluated.
34072122	9	33	theme	co-extruded	1435:1445	arg1	layer					1447:1451	the co-extruded layer	1431:1451	the co-extruded layer	1431:1451	Use of the APP in the co-extruded layer worsened color change compared to the formulation without APP but the pHRR was not significantly changed.
34072122	4	34	theme	UL-94	764:768	arg1	tests					770:774	UL-94 tests	764:774	UL-94 tests	764:774	Various FR for inclusion in the co-extruded layer were screened based on UL-94 tests.
34072122	2	35	theme	straw	405:409	arg1	particles					411:419	wheat straw particles	399:419	wheat straw particles	399:419	In this work, thermoplastic profiles for façade applications based on high-density polyethylene, wheat straw particles, and fire-retardants were extruded and their reaction-to-fire performance before and after artificial weathering evaluated.
34072122	13	36	theme	high	2050:2053	arg1	pHRR					2055:2058	the high pHRR	2046:2058	the high pHRR	2046:2058	However, due to the high pHRR, at best, class D was obtained under the conditions of this study.
34072122	1	37	from	present	115:121	arg1	available					146:154	available	146:154	available	146:154	At present, little information is available in the scientific literature related to the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics.
34072122	0	38	theme	Flame-Retarded	14:27	arg1	Profiles					42:49	Flame-Retarded, Co-Extruded Profiles	14:49	Flame-Retarded, Co-Extruded Profiles	14:49	Durability of Flame-Retarded, Co-Extruded Profiles Based on High-Density Polyethylene and Wheat Straw Residues.
34072122	6	39	theme	heat	1025:1028	arg1	THR					1039:1041	THR	1039:1041	THR	1039:1041	Before weathering, the peak heat release rate (pHRR) and the total heat release (THR), which were determined using cone calorimeter measurements, were reduced by up to 64% and 67% due to the FR.
34072122	6	39	theme	heat	1025:1028	arg1	release					1030:1036	the total heat release	1015:1036	the total heat release (THR)	1015:1042	Before weathering, the peak heat release rate (pHRR) and the total heat release (THR), which were determined using cone calorimeter measurements, were reduced by up to 64% and 67% due to the FR.
34072122	2	40	theme	artificial	512:521	arg1	weathering					523:532	artificial weathering	512:532	artificial weathering	512:532	In this work, thermoplastic profiles for façade applications based on high-density polyethylene, wheat straw particles, and fire-retardants were extruded and their reaction-to-fire performance before and after artificial weathering evaluated.
34072122	6	41	theme	total	1019:1023	arg1	THR					1039:1041	THR	1039:1041	THR	1039:1041	Before weathering, the peak heat release rate (pHRR) and the total heat release (THR), which were determined using cone calorimeter measurements, were reduced by up to 64% and 67% due to the FR.
34072122	6	41	theme	total	1019:1023	arg1	release					1030:1036	the total heat release	1015:1036	the total heat release (THR)	1015:1042	Before weathering, the peak heat release rate (pHRR) and the total heat release (THR), which were determined using cone calorimeter measurements, were reduced by up to 64% and 67% due to the FR.
34072122	0	42	theme	High-Density	60:71	arg1	Polyethylene					73:84	High-Density Polyethylene	60:84	High-Density Polyethylene	60:84	Durability of Flame-Retarded, Co-Extruded Profiles Based on High-Density Polyethylene and Wheat Straw Residues.
34072122	14	43	theme	index	2232:2236	arg1	results					2173:2179	results	2173:2179	results from the single flame source test	2173:2213	In addition to cone calorimeter measurements, results from the single flame source test, limiting oxygen index determination and thermogravimetric analysis, are shown and discussed.
34072122	14	43	theme	index	2232:2236	arg1	determination					2238:2250	limiting oxygen index determination	2216:2250	limiting oxygen index determination	2216:2250	In addition to cone calorimeter measurements, results from the single flame source test, limiting oxygen index determination and thermogravimetric analysis, are shown and discussed.
34072122	5	44	theme	FR	813:814	arg1	types					804:808	two types	800:808	two types of FR	800:814	For profile extrusion, two types of FR were chosen: a coated intumescent combination based on ammonium polyphosphate (APP) and an APP coated with melamine and without formaldehyde.
34072122	14	45	theme	limiting	2216:2223	arg1	results					2173:2179	results	2173:2179	results from the single flame source test	2173:2213	In addition to cone calorimeter measurements, results from the single flame source test, limiting oxygen index determination and thermogravimetric analysis, are shown and discussed.
34072122	14	45	theme	limiting	2216:2223	arg1	determination					2238:2250	limiting oxygen index determination	2216:2250	limiting oxygen index determination	2216:2250	In addition to cone calorimeter measurements, results from the single flame source test, limiting oxygen index determination and thermogravimetric analysis, are shown and discussed.
34072122	10	46	theme	weathering	1576:1585	arg1	small					1612:1616	small	1612:1616	small	1612:1616	The influence of weathering on the fire behavior was small compared to the difference between fire-retarded and non-fire-retarded materials.
34072122	10	46	theme	weathering	1576:1585	arg1	influence					1563:1571	The influence	1559:1571	The influence of weathering on the fire behavior	1559:1606	The influence of weathering on the fire behavior was small compared to the difference between fire-retarded and non-fire-retarded materials.
34072122	0	47	dep	Flame-Retarded	14:27	arg1	Co-Extruded					30:40	Co-Extruded	30:40	Co-Extruded	30:40	Durability of Flame-Retarded, Co-Extruded Profiles Based on High-Density Polyethylene and Wheat Straw Residues.
34072122	6	48	theme	cone	1073:1076	arg1	measurements					1090:1101	cone calorimeter measurements	1073:1101	cone calorimeter measurements	1073:1101	Before weathering, the peak heat release rate (pHRR) and the total heat release (THR), which were determined using cone calorimeter measurements, were reduced by up to 64% and 67% due to the FR.
34072122	10	49	from	influence	1563:1571	arg1	behavior					1599:1606	the fire behavior	1590:1606	the fire behavior	1590:1606	The influence of weathering on the fire behavior was small compared to the difference between fire-retarded and non-fire-retarded materials.
34072122	14	50	theme	flame	2197:2201	arg1	test					2210:2213	the single flame source test	2186:2213	the single flame source test	2186:2213	In addition to cone calorimeter measurements, results from the single flame source test, limiting oxygen index determination and thermogravimetric analysis, are shown and discussed.
34072122	2	51	theme	high-density	372:383	arg1	polyethylene					385:396	high-density polyethylene	372:396	high-density polyethylene	372:396	In this work, thermoplastic profiles for façade applications based on high-density polyethylene, wheat straw particles, and fire-retardants were extruded and their reaction-to-fire performance before and after artificial weathering evaluated.
34072122	1	52	theme	wood	252:255	arg1	durability					200:209	the durability	196:209	the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics	196:299	At present, little information is available in the scientific literature related to the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics.
34072122	1	52	theme	wood	252:255	arg1	resistance					223:232	weathering resistance	212:232	weathering resistance	212:232	At present, little information is available in the scientific literature related to the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics.
34072122	12	53	from	%	1923:1923	arg1	formulations					1877:1888	many formulations	1872:1888	many formulations	1872:1888	In many formulations, increase in THR was less than 20% compared to before weathering, which would place some of the profiles in class C or better (EN 13501-1).
34072122	1	54	theme	natural	261:267	arg1	thermoplastics					286:299	natural fiber-reinforced thermoplastics	261:299	natural fiber-reinforced thermoplastics	261:299	At present, little information is available in the scientific literature related to the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics.
34072122	5	55	dep	chosen	821:826	arg1	combination					850:860	a coated intumescent combination	829:860	a coated intumescent combination based on ammonium polyphosphate (APP) and an APP coated with melamine and without formaldehyde	829:955	For profile extrusion, two types of FR were chosen: a coated intumescent combination based on ammonium polyphosphate (APP) and an APP coated with melamine and without formaldehyde.
34072122	4	56	from	FR	699:700	arg1	layer					735:739	the co-extruded layer	719:739	the co-extruded layer	719:739	Various FR for inclusion in the co-extruded layer were screened based on UL-94 tests.
34072122	1	57	theme	scientific	163:172	arg1	literature					174:183	the scientific literature	159:183	the scientific literature related to the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics	159:299	At present, little information is available in the scientific literature related to the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics.
34072122	12	58	from	increase	1891:1898	arg1	THR					1903:1905	THR	1903:1905	THR	1903:1905	In many formulations, increase in THR was less than 20% compared to before weathering, which would place some of the profiles in class C or better (EN 13501-1).
34072122	10	59	theme	non-fire-retarded	1671:1687	arg1	materials					1689:1697	fire-retarded and non-fire-retarded materials	1653:1697	fire-retarded and non-fire-retarded materials	1653:1697	The influence of weathering on the fire behavior was small compared to the difference between fire-retarded and non-fire-retarded materials.
34072122	1	60	theme	related	185:191	arg1	literature					174:183	the scientific literature	159:183	the scientific literature related to the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics	159:299	At present, little information is available in the scientific literature related to the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics.
34072122	12	61	theme	class	1998:2002	arg1	C					2004:2004	class C	1998:2004	class C	1998:2004	In many formulations, increase in THR was less than 20% compared to before weathering, which would place some of the profiles in class C or better (EN 13501-1).
34072122	14	62	theme	cone	2142:2145	arg1	measurements					2159:2170	cone calorimeter measurements	2142:2170	cone calorimeter measurements	2142:2170	In addition to cone calorimeter measurements, results from the single flame source test, limiting oxygen index determination and thermogravimetric analysis, are shown and discussed.
34072122	10	63	theme	fire-retarded	1653:1665	arg1	materials					1689:1697	fire-retarded and non-fire-retarded materials	1653:1697	fire-retarded and non-fire-retarded materials	1653:1697	The influence of weathering on the fire behavior was small compared to the difference between fire-retarded and non-fire-retarded materials.
34072122	5	64	theme	coated	831:836	arg1	combination					850:860	a coated intumescent combination	829:860	a coated intumescent combination based on ammonium polyphosphate (APP) and an APP coated with melamine and without formaldehyde	829:955	For profile extrusion, two types of FR were chosen: a coated intumescent combination based on ammonium polyphosphate (APP) and an APP coated with melamine and without formaldehyde.
34072122	12	65	theme	many	1872:1875	arg1	formulations					1877:1888	many formulations	1872:1888	many formulations	1872:1888	In many formulations, increase in THR was less than 20% compared to before weathering, which would place some of the profiles in class C or better (EN 13501-1).
34072122	8	66	theme	artificial	1324:1333	arg1	weathering					1335:1344	artificial weathering	1324:1344	artificial weathering	1324:1344	After 28 days of artificial weathering, changes in reaction-to-fire performance and color were evaluated.
34072122	11	67	theme	building	1850:1857	arg1	products					1859:1866	building products	1850:1866	building products	1850:1866	Results from the cone calorimeter were analyzed with regard to ETAG 028, which provides requirements related to the durability of fire performance of building products.
34072122	15	68	theme	dispersive	2406:2415	arg1	data					2423:2426	energy dispersive X-ray data	2399:2426	energy dispersive X-ray data	2399:2426	Strength properties, water uptake and swelling of the profiles, thermal conductivity, and energy dispersive X-ray data are also presented.
34072122	7	69	dep	values	1247:1252	arg1	lowest					1239:1244	lowest	1239:1244	lowest	1239:1244	However, even before weathering, pHRR of the profiles was relatively high, with best (lowest) values between 230 and 250 kW/m2 under the test conditions.
34072122	11	70	theme	performance	1835:1845	arg1	durability					1816:1825	the durability	1812:1825	the durability of fire performance of building products	1812:1866	Results from the cone calorimeter were analyzed with regard to ETAG 028, which provides requirements related to the durability of fire performance of building products.
34072122	11	71	theme	cone	1717:1720	arg1	calorimeter					1722:1732	the cone calorimeter	1713:1732	the cone calorimeter	1713:1732	Results from the cone calorimeter were analyzed with regard to ETAG 028, which provides requirements related to the durability of fire performance of building products.
34072122	3	72	theme	co-extruded	657:667	arg1	layer					669:673	the co-extruded layer	653:673	the co-extruded layer	653:673	Profile geometries were either solid or hollow-core profiles, and fire-retardants (FR) were added either in the co-extruded layer or in the bulk.
34072122	1	73	theme	little	124:129	arg1	information					131:141	little information	124:141	little information	124:141	At present, little information is available in the scientific literature related to the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics.
34072122	6	74	theme	due	1138:1140	arg1	%					1128:1128	up to 64%	1120:1128	up to 64%	1120:1128	Before weathering, the peak heat release rate (pHRR) and the total heat release (THR), which were determined using cone calorimeter measurements, were reduced by up to 64% and 67% due to the FR.
34072122	2	75	theme	façade	343:348	arg1	applications					350:361	façade applications	343:361	façade applications based on high-density polyethylene, wheat straw particles, and fire-retardants	343:440	In this work, thermoplastic profiles for façade applications based on high-density polyethylene, wheat straw particles, and fire-retardants were extruded and their reaction-to-fire performance before and after artificial weathering evaluated.
34072122	14	76	from	test	2210:2213	arg1	analysis					2274:2281	thermogravimetric analysis	2256:2281	thermogravimetric analysis	2256:2281	In addition to cone calorimeter measurements, results from the single flame source test, limiting oxygen index determination and thermogravimetric analysis, are shown and discussed.
34072122	14	76	from	test	2210:2213	arg1	results					2173:2179	results	2173:2179	results from the single flame source test	2173:2213	In addition to cone calorimeter measurements, results from the single flame source test, limiting oxygen index determination and thermogravimetric analysis, are shown and discussed.
34072122	14	76	from	test	2210:2213	arg1	determination					2238:2250	limiting oxygen index determination	2216:2250	limiting oxygen index determination	2216:2250	In addition to cone calorimeter measurements, results from the single flame source test, limiting oxygen index determination and thermogravimetric analysis, are shown and discussed.
34072122	0	77	theme	Wheat	90:94	arg1	Residues					102:109	Wheat Straw Residues	90:109	Wheat Straw Residues	90:109	Durability of Flame-Retarded, Co-Extruded Profiles Based on High-Density Polyethylene and Wheat Straw Residues.
34072122	1	78	from	literature	174:183	arg1	available					146:154	available	146:154	available	146:154	At present, little information is available in the scientific literature related to the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics.
34072122	13	79	theme	class	2070:2074	arg1	D					2076:2076	class D	2070:2076	class D	2070:2076	However, due to the high pHRR, at best, class D was obtained under the conditions of this study.
34072122	9	80	theme	APP	1424:1426	arg1	Use					1413:1415	Use	1413:1415	Use of the APP in the co-extruded layer	1413:1451	Use of the APP in the co-extruded layer worsened color change compared to the formulation without APP but the pHRR was not significantly changed.
34072122	6	81	theme	heat	986:989	arg1	pHRR					1005:1008	pHRR	1005:1008	pHRR	1005:1008	Before weathering, the peak heat release rate (pHRR) and the total heat release (THR), which were determined using cone calorimeter measurements, were reduced by up to 64% and 67% due to the FR.
34072122	6	81	theme	heat	986:989	arg1	rate					999:1002	the peak heat release rate	977:1002	the peak heat release rate (pHRR)	977:1009	Before weathering, the peak heat release rate (pHRR) and the total heat release (THR), which were determined using cone calorimeter measurements, were reduced by up to 64% and 67% due to the FR.
34072122	1	82	from	available	146:154	arg1	literature					174:183	the scientific literature	159:183	the scientific literature related to the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics	159:299	At present, little information is available in the scientific literature related to the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics.
34072122	7	83	theme	best	1233:1236	arg1	values					1247:1252	best (lowest) values	1233:1252	best (lowest) values between 230 and 250 kW/m2	1233:1278	However, even before weathering, pHRR of the profiles was relatively high, with best (lowest) values between 230 and 250 kW/m2 under the test conditions.
34072122	2	84	theme	reaction-to-fire	466:481	arg1	performance					483:493	their reaction-to-fire performance	460:493	their reaction-to-fire performance before and after artificial weathering	460:532	In this work, thermoplastic profiles for façade applications based on high-density polyethylene, wheat straw particles, and fire-retardants were extruded and their reaction-to-fire performance before and after artificial weathering evaluated.
34072122	2	85	theme	wheat	399:403	arg1	particles					411:419	wheat straw particles	399:419	wheat straw particles	399:419	In this work, thermoplastic profiles for façade applications based on high-density polyethylene, wheat straw particles, and fire-retardants were extruded and their reaction-to-fire performance before and after artificial weathering evaluated.
34072122	12	86	from	place	1968:1972	arg1	better					2009:2014	better	2009:2014	better	2009:2014	In many formulations, increase in THR was less than 20% compared to before weathering, which would place some of the profiles in class C or better (EN 13501-1).
34072122	12	86	from	place	1968:1972	arg1	13501-1					2020:2026	EN 13501-1	2017:2026	EN 13501-1	2017:2026	In many formulations, increase in THR was less than 20% compared to before weathering, which would place some of the profiles in class C or better (EN 13501-1).
34072122	12	86	from	place	1968:1972	arg1	C					2004:2004	class C	1998:2004	class C	1998:2004	In many formulations, increase in THR was less than 20% compared to before weathering, which would place some of the profiles in class C or better (EN 13501-1).
34072122	9	87	theme	color	1462:1466	arg1	change					1468:1473	color change	1462:1473	color change	1462:1473	Use of the APP in the co-extruded layer worsened color change compared to the formulation without APP but the pHRR was not significantly changed.
34072122	11	88	theme	fire	1830:1833	arg1	performance					1835:1845	fire performance	1830:1845	fire performance of building products	1830:1866	Results from the cone calorimeter were analyzed with regard to ETAG 028, which provides requirements related to the durability of fire performance of building products.
34072122	0	89	theme	Profiles	42:49	arg1	Durability					0:9	Durability	0:9	Durability of Flame-Retarded, Co-Extruded Profiles	0:49	Durability of Flame-Retarded, Co-Extruded Profiles Based on High-Density Polyethylene and Wheat Straw Residues.
34072122	7	90	theme	profiles	1198:1205	arg1	pHRR					1186:1189	pHRR	1186:1189	pHRR of the profiles	1186:1205	However, even before weathering, pHRR of the profiles was relatively high, with best (lowest) values between 230 and 250 kW/m2 under the test conditions.
34072122	14	91	theme	oxygen	2225:2230	arg1	results					2173:2179	results	2173:2179	results from the single flame source test	2173:2213	In addition to cone calorimeter measurements, results from the single flame source test, limiting oxygen index determination and thermogravimetric analysis, are shown and discussed.
34072122	14	91	theme	oxygen	2225:2230	arg1	determination					2238:2250	limiting oxygen index determination	2216:2250	limiting oxygen index determination	2216:2250	In addition to cone calorimeter measurements, results from the single flame source test, limiting oxygen index determination and thermogravimetric analysis, are shown and discussed.
34072122	3	92	theme	Profile	545:551	arg1	geometries					553:562	Profile geometries	545:562	Profile geometries	545:562	Profile geometries were either solid or hollow-core profiles, and fire-retardants (FR) were added either in the co-extruded layer or in the bulk.
34072122	6	93	theme	calorimeter	1078:1088	arg1	measurements					1090:1101	cone calorimeter measurements	1073:1101	cone calorimeter measurements	1073:1101	Before weathering, the peak heat release rate (pHRR) and the total heat release (THR), which were determined using cone calorimeter measurements, were reduced by up to 64% and 67% due to the FR.
34072122	15	94	theme	thermal	2373:2379	arg1	conductivity					2381:2392	thermal conductivity	2373:2392	thermal conductivity	2373:2392	Strength properties, water uptake and swelling of the profiles, thermal conductivity, and energy dispersive X-ray data are also presented.
34072122	13	95	theme	study	2120:2124	arg1	conditions					2101:2110	the conditions	2097:2110	the conditions of this study	2097:2124	However, due to the high pHRR, at best, class D was obtained under the conditions of this study.
34072122	14	96	theme	source	2203:2208	arg1	test					2210:2213	the single flame source test	2186:2213	the single flame source test	2186:2213	In addition to cone calorimeter measurements, results from the single flame source test, limiting oxygen index determination and thermogravimetric analysis, are shown and discussed.
34072122	3	97	theme	solid	576:580	arg1	profiles					597:604	either solid or hollow-core profiles	569:604	either solid or hollow-core profiles	569:604	Profile geometries were either solid or hollow-core profiles, and fire-retardants (FR) were added either in the co-extruded layer or in the bulk.
34072122	15	98	theme	profiles	2363:2370	arg1	properties					2318:2327	Strength properties	2309:2327	Strength properties	2309:2327	Strength properties, water uptake and swelling of the profiles, thermal conductivity, and energy dispersive X-ray data are also presented.
34072122	15	98	theme	profiles	2363:2370	arg1	conductivity					2381:2392	thermal conductivity	2373:2392	thermal conductivity	2373:2392	Strength properties, water uptake and swelling of the profiles, thermal conductivity, and energy dispersive X-ray data are also presented.
34072122	15	98	theme	profiles	2363:2370	arg1	swelling					2347:2354	swelling	2347:2354	swelling	2347:2354	Strength properties, water uptake and swelling of the profiles, thermal conductivity, and energy dispersive X-ray data are also presented.
34072122	15	98	theme	profiles	2363:2370	arg1	data					2423:2426	energy dispersive X-ray data	2399:2426	energy dispersive X-ray data	2399:2426	Strength properties, water uptake and swelling of the profiles, thermal conductivity, and energy dispersive X-ray data are also presented.
34072122	15	98	theme	profiles	2363:2370	arg1	uptake					2336:2341	water uptake	2330:2341	water uptake	2330:2341	Strength properties, water uptake and swelling of the profiles, thermal conductivity, and energy dispersive X-ray data are also presented.
34072122	1	99	theme	fire-retarded	238:250	arg1	wood					252:255	fire-retarded wood	238:255	fire-retarded wood	238:255	At present, little information is available in the scientific literature related to the durability (weathering resistance) of fire-retarded wood and natural fiber-reinforced thermoplastics.
34432454	7	0	theme	NFP	1058:1060	arg1	related					1081:1087	related	1081:1087	related	1081:1087	Taken together, the mechanism of NFP alleviating IBD is related to the intestinal microecological balance to inhibit inflammatory signaling pathways.
34432454	7	0	theme	NFP	1058:1060	arg1	mechanism					1045:1053	the mechanism	1041:1053	the mechanism of NFP alleviating IBD	1041:1076	Taken together, the mechanism of NFP alleviating IBD is related to the intestinal microecological balance to inhibit inflammatory signaling pathways.
34432454	1	1	theme	Inflammatory	149:160	arg1	IBD					177:179	IBD	177:179	IBD	177:179	Inflammatory bowel disease (IBD) is a disease characterized by intestinal inflammation with immune dysregulation and intestinal microecological imbalance.
34432454	1	1	theme	Inflammatory	149:160	arg1	disease					168:174	Inflammatory bowel disease	149:174	Inflammatory bowel disease (IBD)	149:180	Inflammatory bowel disease (IBD) is a disease characterized by intestinal inflammation with immune dysregulation and intestinal microecological imbalance.
34432454	1	1	theme	Inflammatory	149:160	arg1	disease					187:193	a disease	185:193	a disease characterized by intestinal inflammation with immune dysregulation and intestinal microecological imbalance	185:301	Inflammatory bowel disease (IBD) is a disease characterized by intestinal inflammation with immune dysregulation and intestinal microecological imbalance.
34432454	1	2	theme	immune	241:246	arg1	dysregulation					248:260	immune dysregulation	241:260	immune dysregulation	241:260	Inflammatory bowel disease (IBD) is a disease characterized by intestinal inflammation with immune dysregulation and intestinal microecological imbalance.
34432454	5	3	theme	acid	865:868	arg1	content					831:837	the content	827:837	the content of acetic acid, propionic acid, and butyric acid by onefold	827:897	Targeted metabolomic analysis revealed that NFP upregulated the content of acetic acid, propionic acid, and butyric acid by onefold but reduced isobutyric acid and isovaleric acid contents.
34432454	1	4	theme	bowel	162:166	arg1	IBD					177:179	IBD	177:179	IBD	177:179	Inflammatory bowel disease (IBD) is a disease characterized by intestinal inflammation with immune dysregulation and intestinal microecological imbalance.
34432454	1	4	theme	bowel	162:166	arg1	disease					168:174	Inflammatory bowel disease	149:174	Inflammatory bowel disease (IBD)	149:180	Inflammatory bowel disease (IBD) is a disease characterized by intestinal inflammation with immune dysregulation and intestinal microecological imbalance.
34432454	1	4	theme	bowel	162:166	arg1	disease					187:193	a disease	185:193	a disease characterized by intestinal inflammation with immune dysregulation and intestinal microecological imbalance	185:301	Inflammatory bowel disease (IBD) is a disease characterized by intestinal inflammation with immune dysregulation and intestinal microecological imbalance.
34432454	0	5	theme	Gut	84:86	arg1	Association					100:110	Targeting Gut Microbiota: Association	74:110	Targeting Gut Microbiota: Association of JNK/ERK/NF-κB Signaling Pathways	74:146	Noni (Morinda citrifolia L.) Fruit Polysaccharides Regulated IBD Mice Via Targeting Gut Microbiota: Association of JNK/ERK/NF-κB Signaling Pathways.
34432454	4	6	theme	microbial	723:731	arg1	composition					733:743	the microbial composition	719:743	the microbial composition	719:743	Sequencing analysis of gut microbiota showed an attenuated disruption of the microbial composition in the DSS+NFP group.
34432454	5	7	theme	isobutyric	911:920	arg1	acid					922:925	isobutyric acid	911:925	isobutyric acid	911:925	Targeted metabolomic analysis revealed that NFP upregulated the content of acetic acid, propionic acid, and butyric acid by onefold but reduced isobutyric acid and isovaleric acid contents.
34432454	0	8	theme	Targeting	74:82	arg1	Association					100:110	Targeting Gut Microbiota: Association	74:110	Targeting Gut Microbiota: Association of JNK/ERK/NF-κB Signaling Pathways	74:146	Noni (Morinda citrifolia L.) Fruit Polysaccharides Regulated IBD Mice Via Targeting Gut Microbiota: Association of JNK/ERK/NF-κB Signaling Pathways.
34432454	8	9	theme	patients	1275:1282	arg1	treatment					1258:1266	treatment	1258:1266	treatment	1258:1266	This study provides a basis for NFP as a cheap intervention for the prevention and treatment of IBD patients.
34432454	8	9	theme	patients	1275:1282	arg1	prevention					1243:1252	prevention	1243:1252	prevention	1243:1252	This study provides a basis for NFP as a cheap intervention for the prevention and treatment of IBD patients.
34432454	2	10	theme	IBD	351:353	arg1	model					361:365	a dextran sulfate sodium salt (DSS)-induced IBD mouse model	307:365	a dextran sulfate sodium salt (DSS)-induced IBD mouse model	307:365	In a dextran sulfate sodium salt (DSS)-induced IBD mouse model, noni (Morinda citrifolia L.) fruit polysaccharides (NFP) with homogalacturonan and rhamnogalacturonan-I domain decreased the concentration of serum LPS, TNF-α, and IL-17 by 84, 42, and 65%, respectively.
34432454	1	11	theme	intestinal	266:275	arg1	imbalance					293:301	intestinal microecological imbalance	266:301	intestinal microecological imbalance	266:301	Inflammatory bowel disease (IBD) is a disease characterized by intestinal inflammation with immune dysregulation and intestinal microecological imbalance.
34432454	2	12	theme	serum	510:514	arg1	LPS					516:518	serum LPS	510:518	serum LPS	510:518	In a dextran sulfate sodium salt (DSS)-induced IBD mouse model, noni (Morinda citrifolia L.) fruit polysaccharides (NFP) with homogalacturonan and rhamnogalacturonan-I domain decreased the concentration of serum LPS, TNF-α, and IL-17 by 84, 42, and 65%, respectively.
34432454	5	13	theme	isovaleric	931:940	arg1	acid					942:945	isovaleric acid	931:945	isovaleric acid	931:945	Targeted metabolomic analysis revealed that NFP upregulated the content of acetic acid, propionic acid, and butyric acid by onefold but reduced isobutyric acid and isovaleric acid contents.
34432454	5	14	theme	acetic	842:847	arg1	acid					849:852	acetic acid	842:852	acetic acid	842:852	Targeted metabolomic analysis revealed that NFP upregulated the content of acetic acid, propionic acid, and butyric acid by onefold but reduced isobutyric acid and isovaleric acid contents.
34432454	8	15	theme	IBD	1271:1273	arg1	patients					1275:1282	IBD patients	1271:1282	IBD patients	1271:1282	This study provides a basis for NFP as a cheap intervention for the prevention and treatment of IBD patients.
34432454	0	16	theme	Microbiota	88:97	arg1	Association					100:110	Targeting Gut Microbiota: Association	74:110	Targeting Gut Microbiota: Association of JNK/ERK/NF-κB Signaling Pathways	74:146	Noni (Morinda citrifolia L.) Fruit Polysaccharides Regulated IBD Mice Via Targeting Gut Microbiota: Association of JNK/ERK/NF-κB Signaling Pathways.
34432454	4	17	theme	composition	733:743	arg1	disruption					705:714	an attenuated disruption	691:714	an attenuated disruption of the microbial composition in the DSS+NFP group	691:764	Sequencing analysis of gut microbiota showed an attenuated disruption of the microbial composition in the DSS+NFP group.
34432454	6	18	theme	mice	1019:1022	arg1	phosphorylation					996:1010	JNK, ERK, and NF-κB phosphorylation	976:1010	JNK, ERK, and NF-κB phosphorylation of IBD mice	976:1022	NFP also inhibited JNK, ERK, and NF-κB phosphorylation of IBD mice.
34432454	5	19	theme	Targeted	767:774	arg1	analysis					788:795	Targeted metabolomic analysis	767:795	Targeted metabolomic analysis	767:795	Targeted metabolomic analysis revealed that NFP upregulated the content of acetic acid, propionic acid, and butyric acid by onefold but reduced isobutyric acid and isovaleric acid contents.
34432454	4	20	from	disruption	705:714	arg1	group					760:764	the DSS+NFP group	748:764	the DSS+NFP group	748:764	Sequencing analysis of gut microbiota showed an attenuated disruption of the microbial composition in the DSS+NFP group.
34432454	4	21	theme	attenuated	694:703	arg1	disruption					705:714	an attenuated disruption	691:714	an attenuated disruption of the microbial composition in the DSS+NFP group	691:764	Sequencing analysis of gut microbiota showed an attenuated disruption of the microbial composition in the DSS+NFP group.
34432454	7	22	theme	microecological	1107:1121	arg1	balance					1123:1129	the intestinal microecological balance	1092:1129	the intestinal microecological balance	1092:1129	Taken together, the mechanism of NFP alleviating IBD is related to the intestinal microecological balance to inhibit inflammatory signaling pathways.
34432454	2	23	theme	noni	368:371	arg1	NFP					420:422	NFP	420:422	NFP	420:422	In a dextran sulfate sodium salt (DSS)-induced IBD mouse model, noni (Morinda citrifolia L.) fruit polysaccharides (NFP) with homogalacturonan and rhamnogalacturonan-I domain decreased the concentration of serum LPS, TNF-α, and IL-17 by 84, 42, and 65%, respectively.
34432454	2	23	theme	noni	368:371	arg1	polysaccharides					403:417	noni (Morinda citrifolia L.) fruit polysaccharides	368:417	noni (Morinda citrifolia L.) fruit polysaccharides (NFP) with homogalacturonan and rhamnogalacturonan-I domain	368:477	In a dextran sulfate sodium salt (DSS)-induced IBD mouse model, noni (Morinda citrifolia L.) fruit polysaccharides (NFP) with homogalacturonan and rhamnogalacturonan-I domain decreased the concentration of serum LPS, TNF-α, and IL-17 by 84, 42, and 65%, respectively.
34432454	5	24	theme	metabolomic	776:786	arg1	analysis					788:795	Targeted metabolomic analysis	767:795	Targeted metabolomic analysis	767:795	Targeted metabolomic analysis revealed that NFP upregulated the content of acetic acid, propionic acid, and butyric acid by onefold but reduced isobutyric acid and isovaleric acid contents.
34432454	1	25	theme	microecological	277:291	arg1	imbalance					293:301	intestinal microecological imbalance	266:301	intestinal microecological imbalance	266:301	Inflammatory bowel disease (IBD) is a disease characterized by intestinal inflammation with immune dysregulation and intestinal microecological imbalance.
34432454	4	26	theme	Sequencing	646:655	arg1	analysis					657:664	Sequencing analysis	646:664	Sequencing analysis of gut microbiota	646:682	Sequencing analysis of gut microbiota showed an attenuated disruption of the microbial composition in the DSS+NFP group.
34432454	2	27	theme	IL-17	532:536	arg1	concentration					493:505	the concentration	489:505	the concentration of serum LPS, TNF-α, and IL-17	489:536	In a dextran sulfate sodium salt (DSS)-induced IBD mouse model, noni (Morinda citrifolia L.) fruit polysaccharides (NFP) with homogalacturonan and rhamnogalacturonan-I domain decreased the concentration of serum LPS, TNF-α, and IL-17 by 84, 42, and 65%, respectively.
34432454	2	28	theme	fruit	397:401	arg1	NFP					420:422	NFP	420:422	NFP	420:422	In a dextran sulfate sodium salt (DSS)-induced IBD mouse model, noni (Morinda citrifolia L.) fruit polysaccharides (NFP) with homogalacturonan and rhamnogalacturonan-I domain decreased the concentration of serum LPS, TNF-α, and IL-17 by 84, 42, and 65%, respectively.
34432454	2	28	theme	fruit	397:401	arg1	polysaccharides					403:417	noni (Morinda citrifolia L.) fruit polysaccharides	368:417	noni (Morinda citrifolia L.) fruit polysaccharides (NFP) with homogalacturonan and rhamnogalacturonan-I domain	368:477	In a dextran sulfate sodium salt (DSS)-induced IBD mouse model, noni (Morinda citrifolia L.) fruit polysaccharides (NFP) with homogalacturonan and rhamnogalacturonan-I domain decreased the concentration of serum LPS, TNF-α, and IL-17 by 84, 42, and 65%, respectively.
34432454	7	29	theme	intestinal	1096:1105	arg1	balance					1123:1129	the intestinal microecological balance	1092:1129	the intestinal microecological balance	1092:1129	Taken together, the mechanism of NFP alleviating IBD is related to the intestinal microecological balance to inhibit inflammatory signaling pathways.
34432454	2	30	theme	-induced	342:349	arg1	model					361:365	a dextran sulfate sodium salt (DSS)-induced IBD mouse model	307:365	a dextran sulfate sodium salt (DSS)-induced IBD mouse model	307:365	In a dextran sulfate sodium salt (DSS)-induced IBD mouse model, noni (Morinda citrifolia L.) fruit polysaccharides (NFP) with homogalacturonan and rhamnogalacturonan-I domain decreased the concentration of serum LPS, TNF-α, and IL-17 by 84, 42, and 65%, respectively.
34432454	6	31	theme	NF-κB	990:994	arg1	phosphorylation					996:1010	JNK, ERK, and NF-κB phosphorylation	976:1010	JNK, ERK, and NF-κB phosphorylation of IBD mice	976:1022	NFP also inhibited JNK, ERK, and NF-κB phosphorylation of IBD mice.
34432454	2	32	theme	rhamnogalacturonan-I	451:470	arg1	domain					472:477	rhamnogalacturonan-I domain	451:477	rhamnogalacturonan-I domain	451:477	In a dextran sulfate sodium salt (DSS)-induced IBD mouse model, noni (Morinda citrifolia L.) fruit polysaccharides (NFP) with homogalacturonan and rhamnogalacturonan-I domain decreased the concentration of serum LPS, TNF-α, and IL-17 by 84, 42, and 65%, respectively.
34432454	5	33	theme	acid	922:925	arg1	contents					947:954	isobutyric acid and isovaleric acid contents	911:954	isobutyric acid and isovaleric acid contents	911:954	Targeted metabolomic analysis revealed that NFP upregulated the content of acetic acid, propionic acid, and butyric acid by onefold but reduced isobutyric acid and isovaleric acid contents.
34432454	0	34	theme	Signaling	129:137	arg1	Pathways					139:146	JNK/ERK/NF-κB Signaling Pathways	115:146	JNK/ERK/NF-κB Signaling Pathways	115:146	Noni (Morinda citrifolia L.) Fruit Polysaccharides Regulated IBD Mice Via Targeting Gut Microbiota: Association of JNK/ERK/NF-κB Signaling Pathways.
34432454	3	35	theme	intestinal	594:603	arg1	microbiota					605:614	intestinal microbiota	594:614	intestinal microbiota	594:614	It was abolished when intestinal microbiota were depleted by antibiotics.
34432454	5	36	theme	acid	849:852	arg1	content					831:837	the content	827:837	the content of acetic acid, propionic acid, and butyric acid by onefold	827:897	Targeted metabolomic analysis revealed that NFP upregulated the content of acetic acid, propionic acid, and butyric acid by onefold but reduced isobutyric acid and isovaleric acid contents.
34432454	8	37	theme	cheap	1216:1220	arg1	basis					1197:1201	a basis	1195:1201	a basis for NFP	1195:1209	This study provides a basis for NFP as a cheap intervention for the prevention and treatment of IBD patients.
34432454	8	37	theme	cheap	1216:1220	arg1	intervention					1222:1233	a cheap intervention	1214:1233	a cheap intervention for the prevention and treatment of IBD patients	1214:1282	This study provides a basis for NFP as a cheap intervention for the prevention and treatment of IBD patients.
34432454	0	38	theme	JNK/ERK/NF-κB	115:127	arg1	Pathways					139:146	JNK/ERK/NF-κB Signaling Pathways	115:146	JNK/ERK/NF-κB Signaling Pathways	115:146	Noni (Morinda citrifolia L.) Fruit Polysaccharides Regulated IBD Mice Via Targeting Gut Microbiota: Association of JNK/ERK/NF-κB Signaling Pathways.
34432454	6	39	theme	IBD	1015:1017	arg1	mice					1019:1022	IBD mice	1015:1022	IBD mice	1015:1022	NFP also inhibited JNK, ERK, and NF-κB phosphorylation of IBD mice.
34432454	7	40	theme	inflammatory	1142:1153	arg1	pathways					1165:1172	inflammatory signaling pathways	1142:1172	inflammatory signaling pathways	1142:1172	Taken together, the mechanism of NFP alleviating IBD is related to the intestinal microecological balance to inhibit inflammatory signaling pathways.
34432454	2	41	with	polysaccharides	403:417	arg1	homogalacturonan					430:445	homogalacturonan	430:445	homogalacturonan	430:445	In a dextran sulfate sodium salt (DSS)-induced IBD mouse model, noni (Morinda citrifolia L.) fruit polysaccharides (NFP) with homogalacturonan and rhamnogalacturonan-I domain decreased the concentration of serum LPS, TNF-α, and IL-17 by 84, 42, and 65%, respectively.
34432454	2	41	with	polysaccharides	403:417	arg1	domain					472:477	rhamnogalacturonan-I domain	451:477	rhamnogalacturonan-I domain	451:477	In a dextran sulfate sodium salt (DSS)-induced IBD mouse model, noni (Morinda citrifolia L.) fruit polysaccharides (NFP) with homogalacturonan and rhamnogalacturonan-I domain decreased the concentration of serum LPS, TNF-α, and IL-17 by 84, 42, and 65%, respectively.
34432454	4	42	theme	gut	669:671	arg1	microbiota					673:682	gut microbiota	669:682	gut microbiota	669:682	Sequencing analysis of gut microbiota showed an attenuated disruption of the microbial composition in the DSS+NFP group.
34432454	2	43	theme	TNF-α	521:525	arg1	concentration					493:505	the concentration	489:505	the concentration of serum LPS, TNF-α, and IL-17	489:536	In a dextran sulfate sodium salt (DSS)-induced IBD mouse model, noni (Morinda citrifolia L.) fruit polysaccharides (NFP) with homogalacturonan and rhamnogalacturonan-I domain decreased the concentration of serum LPS, TNF-α, and IL-17 by 84, 42, and 65%, respectively.
34432454	0	44	theme	Pathways	139:146	arg1	Association					100:110	Targeting Gut Microbiota: Association	74:110	Targeting Gut Microbiota: Association of JNK/ERK/NF-κB Signaling Pathways	74:146	Noni (Morinda citrifolia L.) Fruit Polysaccharides Regulated IBD Mice Via Targeting Gut Microbiota: Association of JNK/ERK/NF-κB Signaling Pathways.
34432454	7	45	theme	signaling	1155:1163	arg1	pathways					1165:1172	inflammatory signaling pathways	1142:1172	inflammatory signaling pathways	1142:1172	Taken together, the mechanism of NFP alleviating IBD is related to the intestinal microecological balance to inhibit inflammatory signaling pathways.
34432454	5	46	theme	butyric	875:881	arg1	acid					883:886	butyric acid	875:886	butyric acid	875:886	Targeted metabolomic analysis revealed that NFP upregulated the content of acetic acid, propionic acid, and butyric acid by onefold but reduced isobutyric acid and isovaleric acid contents.
34432454	8	47	dep	prevention	1243:1252	arg1	the					1239:1241	the	1239:1241	the	1239:1241	This study provides a basis for NFP as a cheap intervention for the prevention and treatment of IBD patients.
34432454	4	48	theme	DSS+NFP	752:758	arg1	group					760:764	the DSS+NFP group	748:764	the DSS+NFP group	748:764	Sequencing analysis of gut microbiota showed an attenuated disruption of the microbial composition in the DSS+NFP group.
34432454	5	49	theme	acid	883:886	arg1	content					831:837	the content	827:837	the content of acetic acid, propionic acid, and butyric acid by onefold	827:897	Targeted metabolomic analysis revealed that NFP upregulated the content of acetic acid, propionic acid, and butyric acid by onefold but reduced isobutyric acid and isovaleric acid contents.
34432454	6	50	theme	ERK	981:983	arg1	phosphorylation					996:1010	JNK, ERK, and NF-κB phosphorylation	976:1010	JNK, ERK, and NF-κB phosphorylation of IBD mice	976:1022	NFP also inhibited JNK, ERK, and NF-κB phosphorylation of IBD mice.
34432454	5	51	theme	acid	942:945	arg1	contents					947:954	isobutyric acid and isovaleric acid contents	911:954	isobutyric acid and isovaleric acid contents	911:954	Targeted metabolomic analysis revealed that NFP upregulated the content of acetic acid, propionic acid, and butyric acid by onefold but reduced isobutyric acid and isovaleric acid contents.
34432454	0	52	dep	Morinda	6:12	arg1	L.					25:26	Morinda citrifolia L.	6:26	Morinda citrifolia L.	6:26	Noni (Morinda citrifolia L.) Fruit Polysaccharides Regulated IBD Mice Via Targeting Gut Microbiota: Association of JNK/ERK/NF-κB Signaling Pathways.
34432454	1	53	with	inflammation	223:234	arg1	dysregulation					248:260	immune dysregulation	241:260	immune dysregulation	241:260	Inflammatory bowel disease (IBD) is a disease characterized by intestinal inflammation with immune dysregulation and intestinal microecological imbalance.
34432454	1	53	with	inflammation	223:234	arg1	imbalance					293:301	intestinal microecological imbalance	266:301	intestinal microecological imbalance	266:301	Inflammatory bowel disease (IBD) is a disease characterized by intestinal inflammation with immune dysregulation and intestinal microecological imbalance.
34432454	4	54	theme	microbiota	673:682	arg1	analysis					657:664	Sequencing analysis	646:664	Sequencing analysis of gut microbiota	646:682	Sequencing analysis of gut microbiota showed an attenuated disruption of the microbial composition in the DSS+NFP group.
34432454	1	55	theme	intestinal	212:221	arg1	inflammation					223:234	intestinal inflammation	212:234	intestinal inflammation with immune dysregulation and intestinal microecological imbalance	212:301	Inflammatory bowel disease (IBD) is a disease characterized by intestinal inflammation with immune dysregulation and intestinal microecological imbalance.
34432454	0	56	theme	IBD	61:63	arg1	Mice					65:68	Polysaccharides Regulated IBD Mice	35:68	Polysaccharides Regulated IBD Mice	35:68	Noni (Morinda citrifolia L.) Fruit Polysaccharides Regulated IBD Mice Via Targeting Gut Microbiota: Association of JNK/ERK/NF-κB Signaling Pathways.
34432454	2	57	dep	Morinda	374:380	arg1	L.					393:394	Morinda citrifolia L.	374:394	Morinda citrifolia L.	374:394	In a dextran sulfate sodium salt (DSS)-induced IBD mouse model, noni (Morinda citrifolia L.) fruit polysaccharides (NFP) with homogalacturonan and rhamnogalacturonan-I domain decreased the concentration of serum LPS, TNF-α, and IL-17 by 84, 42, and 65%, respectively.
34432454	5	58	theme	propionic	855:863	arg1	acid					865:868	propionic acid	855:868	propionic acid	855:868	Targeted metabolomic analysis revealed that NFP upregulated the content of acetic acid, propionic acid, and butyric acid by onefold but reduced isobutyric acid and isovaleric acid contents.
34432454	6	59	theme	JNK	976:978	arg1	phosphorylation					996:1010	JNK, ERK, and NF-κB phosphorylation	976:1010	JNK, ERK, and NF-κB phosphorylation of IBD mice	976:1022	NFP also inhibited JNK, ERK, and NF-κB phosphorylation of IBD mice.
34432454	0	60	theme	Regulated	51:59	arg1	Mice					65:68	Polysaccharides Regulated IBD Mice	35:68	Polysaccharides Regulated IBD Mice	35:68	Noni (Morinda citrifolia L.) Fruit Polysaccharides Regulated IBD Mice Via Targeting Gut Microbiota: Association of JNK/ERK/NF-κB Signaling Pathways.
34432454	2	61	theme	mouse	355:359	arg1	model					361:365	a dextran sulfate sodium salt (DSS)-induced IBD mouse model	307:365	a dextran sulfate sodium salt (DSS)-induced IBD mouse model	307:365	In a dextran sulfate sodium salt (DSS)-induced IBD mouse model, noni (Morinda citrifolia L.) fruit polysaccharides (NFP) with homogalacturonan and rhamnogalacturonan-I domain decreased the concentration of serum LPS, TNF-α, and IL-17 by 84, 42, and 65%, respectively.
34432454	2	62	theme	LPS	516:518	arg1	concentration					493:505	the concentration	489:505	the concentration of serum LPS, TNF-α, and IL-17	489:536	In a dextran sulfate sodium salt (DSS)-induced IBD mouse model, noni (Morinda citrifolia L.) fruit polysaccharides (NFP) with homogalacturonan and rhamnogalacturonan-I domain decreased the concentration of serum LPS, TNF-α, and IL-17 by 84, 42, and 65%, respectively.
33402867	0	0	theme	arthritis	80:88	arg1	activity					98:105	rheumatoid arthritis disease activity	69:105	rheumatoid arthritis disease activity	69:105	Sex effect on the correlation of immunoglobulin G glycosylation with rheumatoid arthritis disease activity.
33402867	5	1	theme	core-fucosylation	705:721	arg1	G0FB					790:793	G0FB	790:793	G0FB	790:793	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	5	1	theme	core-fucosylation	705:721	arg1	moieties					765:772	core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties	705:772	core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB	705:793	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	5	1	theme	core-fucosylation	705:721	arg1	G0F					782:784	G0F	782:784	G0F	782:784	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	7	2	theme	inflammation	1017:1028	arg1	level					994:998	the level	990:998	the level of IgG-dependent inflammation	990:1028	Here, it is hypothesized that the regulation of G0F by estrogen contributes to sex dichotomy in RA by setting up the level of IgG-dependent inflammation and therefore, RA disease activity (Das28-CRP3).
33402867	1	3	theme	chronic	139:145	arg1	disease					158:164	a chronic autoimmune disease	137:164	a chronic autoimmune disease which affects females more than males with a presence of autoantibodies	137:236	Rheumatoid arthritis (RA) is a chronic autoimmune disease which affects females more than males with a presence of autoantibodies.
33402867	1	3	theme	chronic	139:145	arg1	arthritis					119:127	Rheumatoid arthritis	108:127	Rheumatoid arthritis (RA)	108:132	Rheumatoid arthritis (RA) is a chronic autoimmune disease which affects females more than males with a presence of autoantibodies.
33402867	7	4	from	dichotomy	960:968	arg1	RA					973:974	RA	973:974	RA	973:974	Here, it is hypothesized that the regulation of G0F by estrogen contributes to sex dichotomy in RA by setting up the level of IgG-dependent inflammation and therefore, RA disease activity (Das28-CRP3).
33402867	0	5	theme	rheumatoid	69:78	arg1	arthritis					80:88	rheumatoid arthritis	69:88	rheumatoid arthritis disease activity	69:105	Sex effect on the correlation of immunoglobulin G glycosylation with rheumatoid arthritis disease activity.
33402867	0	6	from	effect	4:9	arg1	correlation					18:28	the correlation	14:28	the correlation of immunoglobulin G glycosylation with rheumatoid arthritis disease activity	14:105	Sex effect on the correlation of immunoglobulin G glycosylation with rheumatoid arthritis disease activity.
33402867	8	7	theme	IgG	1111:1113	arg1	glycosylation					1115:1127	IgG glycosylation	1111:1127	IgG glycosylation	1111:1127	To investigate this hypothesis, IgG glycosylation was characterized in serum samples from active RA patients (n = 232) and healthy controls (n = 232) by serum N-glycan analysis using the high performance liquid chromatography.
33402867	1	8	theme	autoimmune	147:156	arg1	disease					158:164	a chronic autoimmune disease	137:164	a chronic autoimmune disease which affects females more than males with a presence of autoantibodies	137:236	Rheumatoid arthritis (RA) is a chronic autoimmune disease which affects females more than males with a presence of autoantibodies.
33402867	1	8	theme	autoimmune	147:156	arg1	arthritis					119:127	Rheumatoid arthritis	108:127	Rheumatoid arthritis (RA)	108:132	Rheumatoid arthritis (RA) is a chronic autoimmune disease which affects females more than males with a presence of autoantibodies.
33402867	10	9	theme	IgG	1669:1671	arg1	glycosylation					1673:1685	IgG glycosylation	1669:1685	IgG glycosylation	1669:1685	In conclusion, IgG G0F-dependent inflammation differs in males and females, and these differences point to the differential regulation of inflammation by sex hormone estrogen via IgG glycosylation.
33402867	5	10	mod	modified	693:700	arg1	G2					671:672	digalactosylated G2	654:672	digalactosylated G2	654:672	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	5	10	mod	modified	693:700	arg1	G0					623:624	agalactosylated G0	607:624	agalactosylated G0	607:624	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	5	10	mod	modified	693:700	arg1	glycans					581:587	These glycans	575:587	These glycans	575:587	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	5	10	mod	modified	693:700	arg3	G0F					782:784	G0F	782:784	G0F	782:784	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	5	10	mod	modified	693:700	arg3	moieties					765:772	core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties	705:772	core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB	705:793	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	5	10	mod	modified	693:700	arg3	G0FB					790:793	G0FB	790:793	G0FB	790:793	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	5	10	mod	modified	693:700	arg1	G1					646:647	monogalactosylated G1	627:647	monogalactosylated G1	627:647	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	2	11	theme	adaptive	274:281	arg1	arm					283:285	adaptive arm	274:285	adaptive arm	274:285	Immunoglobulin G (IgG) produced by adaptive arm has 2 functional domains, Fc and Fab.
33402867	8	12	from	patients	1179:1186	arg1	samples					1156:1162	serum samples	1150:1162	serum samples from active RA patients (n = 232) and healthy controls (n = 232)	1150:1227	To investigate this hypothesis, IgG glycosylation was characterized in serum samples from active RA patients (n = 232) and healthy controls (n = 232) by serum N-glycan analysis using the high performance liquid chromatography.
33402867	0	13	theme	disease	90:96	arg1	activity					98:105	rheumatoid arthritis disease activity	69:105	rheumatoid arthritis disease activity	69:105	Sex effect on the correlation of immunoglobulin G glycosylation with rheumatoid arthritis disease activity.
33402867	4	14	theme	IgG	415:417	arg1	domain					422:427	the IgG Fc domain	411:427	the IgG Fc domain	411:427	Therefore, the IgG Fc domain serves as a bridge between the innate and adaptive arms and is regulated by an evolutionarily conserved N-glycosylation with variable structures.
33402867	4	14	theme	IgG	415:417	arg1	bridge					441:446	a bridge	439:446	a bridge between the innate and adaptive arms	439:483	Therefore, the IgG Fc domain serves as a bridge between the innate and adaptive arms and is regulated by an evolutionarily conserved N-glycosylation with variable structures.
33402867	5	15	theme	agalactosylated	607:621	arg1	G0					623:624	agalactosylated G0	607:624	agalactosylated G0	607:624	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	5	15	theme	agalactosylated	607:621	arg1	glycans					581:587	These glycans	575:587	These glycans	575:587	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	8	16	theme	healthy	1202:1208	arg1	n					1220:1220	n = 232	1220:1226	n = 232	1220:1226	To investigate this hypothesis, IgG glycosylation was characterized in serum samples from active RA patients (n = 232) and healthy controls (n = 232) by serum N-glycan analysis using the high performance liquid chromatography.
33402867	8	16	theme	healthy	1202:1208	arg1	controls					1210:1217	healthy controls	1202:1217	healthy controls (n = 232)	1202:1227	To investigate this hypothesis, IgG glycosylation was characterized in serum samples from active RA patients (n = 232) and healthy controls (n = 232) by serum N-glycan analysis using the high performance liquid chromatography.
33402867	7	17	theme	IgG-dependent	1003:1015	arg1	inflammation					1017:1028	IgG-dependent inflammation	1003:1028	IgG-dependent inflammation	1003:1028	Here, it is hypothesized that the regulation of G0F by estrogen contributes to sex dichotomy in RA by setting up the level of IgG-dependent inflammation and therefore, RA disease activity (Das28-CRP3).
33402867	0	18	with	glycosylation	50:62	arg1	activity					98:105	rheumatoid arthritis disease activity	69:105	rheumatoid arthritis disease activity	69:105	Sex effect on the correlation of immunoglobulin G glycosylation with rheumatoid arthritis disease activity.
33402867	8	19	theme	=	1222:1222	arg1	n					1220:1220	n = 232	1220:1226	n = 232	1220:1226	To investigate this hypothesis, IgG glycosylation was characterized in serum samples from active RA patients (n = 232) and healthy controls (n = 232) by serum N-glycan analysis using the high performance liquid chromatography.
33402867	8	19	theme	=	1222:1222	arg1	controls					1210:1217	healthy controls	1202:1217	healthy controls (n = 232)	1202:1227	To investigate this hypothesis, IgG glycosylation was characterized in serum samples from active RA patients (n = 232) and healthy controls (n = 232) by serum N-glycan analysis using the high performance liquid chromatography.
33402867	5	20	theme	bisecting	731:739	arg1	B					762:762	B	762:762	B	762:762	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	5	20	theme	bisecting	731:739	arg1	N-acetylglucosamine					741:759	bisecting N-acetylglucosamine	731:759	bisecting N-acetylglucosamine (B)	731:763	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	6	21	theme	proinflammatory	811:825	arg1	G0F					827:829	proinflammatory G0F	811:829	proinflammatory G0F	811:829	Interestingly, proinflammatory G0F is shown to be regulated by estrogen in vivo.
33402867	3	22	theme	innate	388:393	arg1	arm					395:397	the innate arm	384:397	the innate arm	384:397	The Fc domain binds Fc gamma receptors and C1q proteins of the innate arm.
33402867	5	23	theme	N-acetylglucosamine	741:759	arg1	G0FB					790:793	G0FB	790:793	G0FB	790:793	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	5	23	theme	N-acetylglucosamine	741:759	arg1	moieties					765:772	core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties	705:772	core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB	705:793	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	5	23	theme	N-acetylglucosamine	741:759	arg1	G0F					782:784	G0F	782:784	G0F	782:784	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	4	24	theme	Fc	419:420	arg1	domain					422:427	the IgG Fc domain	411:427	the IgG Fc domain	411:427	Therefore, the IgG Fc domain serves as a bridge between the innate and adaptive arms and is regulated by an evolutionarily conserved N-glycosylation with variable structures.
33402867	4	24	theme	Fc	419:420	arg1	bridge					441:446	a bridge	439:446	a bridge between the innate and adaptive arms	439:483	Therefore, the IgG Fc domain serves as a bridge between the innate and adaptive arms and is regulated by an evolutionarily conserved N-glycosylation with variable structures.
33402867	8	25	theme	serum	1150:1154	arg1	samples					1156:1162	serum samples	1150:1162	serum samples from active RA patients (n = 232) and healthy controls (n = 232)	1150:1227	To investigate this hypothesis, IgG glycosylation was characterized in serum samples from active RA patients (n = 232) and healthy controls (n = 232) by serum N-glycan analysis using the high performance liquid chromatography.
33402867	3	26	theme	Fc	345:346	arg1	receptors					354:362	Fc gamma receptors	345:362	Fc gamma receptors	345:362	The Fc domain binds Fc gamma receptors and C1q proteins of the innate arm.
33402867	9	27	from	Das28-CRP3	1449:1458	arg1	males					1483:1487	males	1483:1487	males	1483:1487	According to the results, the IgG Fc glycan phenotype originates predominantly from the structure of G0F, and both G0F and G0FB correlate with Das28-CRP3 in females, but not in males.
33402867	9	27	from	Das28-CRP3	1449:1458	arg1	females					1463:1469	females	1463:1469	females	1463:1469	According to the results, the IgG Fc glycan phenotype originates predominantly from the structure of G0F, and both G0F and G0FB correlate with Das28-CRP3 in females, but not in males.
33402867	3	28	theme	Fc	329:330	arg1	domain					332:337	The Fc domain	325:337	The Fc domain	325:337	The Fc domain binds Fc gamma receptors and C1q proteins of the innate arm.
33402867	4	29	theme	adaptive	471:478	arg1	arms					480:483	the innate and adaptive arms	456:483	the innate and adaptive arms	456:483	Therefore, the IgG Fc domain serves as a bridge between the innate and adaptive arms and is regulated by an evolutionarily conserved N-glycosylation with variable structures.
33402867	1	30	with	males	198:202	arg1	presence					211:218	a presence	209:218	a presence of autoantibodies	209:236	Rheumatoid arthritis (RA) is a chronic autoimmune disease which affects females more than males with a presence of autoantibodies.
33402867	8	31	gly	glycosylation	1115:1127	arg1	samples					1156:1162	serum samples	1150:1162	serum samples from active RA patients (n = 232) and healthy controls (n = 232)	1150:1227	To investigate this hypothesis, IgG glycosylation was characterized in serum samples from active RA patients (n = 232) and healthy controls (n = 232) by serum N-glycan analysis using the high performance liquid chromatography.
33402867	8	32	theme	serum	1232:1236	arg1	analysis					1247:1254	serum N-glycan analysis	1232:1254	serum N-glycan analysis using the high performance liquid chromatography	1232:1303	To investigate this hypothesis, IgG glycosylation was characterized in serum samples from active RA patients (n = 232) and healthy controls (n = 232) by serum N-glycan analysis using the high performance liquid chromatography.
33402867	1	33	theme	more	188:191	arg1	females					180:186	females	180:186	females more than males with a presence of autoantibodies	180:236	Rheumatoid arthritis (RA) is a chronic autoimmune disease which affects females more than males with a presence of autoantibodies.
33402867	7	34	dep	level	994:998	arg1	up					987:988	up	987:988	up	987:988	Here, it is hypothesized that the regulation of G0F by estrogen contributes to sex dichotomy in RA by setting up the level of IgG-dependent inflammation and therefore, RA disease activity (Das28-CRP3).
33402867	1	35	theme	Rheumatoid	108:117	arg1	RA					130:131	RA	130:131	RA	130:131	Rheumatoid arthritis (RA) is a chronic autoimmune disease which affects females more than males with a presence of autoantibodies.
33402867	1	35	theme	Rheumatoid	108:117	arg1	arthritis					119:127	Rheumatoid arthritis	108:127	Rheumatoid arthritis (RA)	108:132	Rheumatoid arthritis (RA) is a chronic autoimmune disease which affects females more than males with a presence of autoantibodies.
33402867	1	35	theme	Rheumatoid	108:117	arg1	disease					158:164	a chronic autoimmune disease	137:164	a chronic autoimmune disease which affects females more than males with a presence of autoantibodies	137:236	Rheumatoid arthritis (RA) is a chronic autoimmune disease which affects females more than males with a presence of autoantibodies.
33402867	10	36	theme	IgG	1505:1507	arg1	inflammation					1523:1534	IgG G0F-dependent inflammation	1505:1534	IgG G0F-dependent inflammation	1505:1534	In conclusion, IgG G0F-dependent inflammation differs in males and females, and these differences point to the differential regulation of inflammation by sex hormone estrogen via IgG glycosylation.
33402867	7	37	theme	RA	1045:1046	arg1	Das28-CRP3					1066:1075	Das28-CRP3	1066:1075	Das28-CRP3	1066:1075	Here, it is hypothesized that the regulation of G0F by estrogen contributes to sex dichotomy in RA by setting up the level of IgG-dependent inflammation and therefore, RA disease activity (Das28-CRP3).
33402867	7	37	theme	RA	1045:1046	arg1	activity					1056:1063	RA disease activity	1045:1063	RA disease activity (Das28-CRP3)	1045:1076	Here, it is hypothesized that the regulation of G0F by estrogen contributes to sex dichotomy in RA by setting up the level of IgG-dependent inflammation and therefore, RA disease activity (Das28-CRP3).
33402867	5	38	theme	digalactosylated	654:669	arg1	glycans					581:587	These glycans	575:587	These glycans	575:587	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	5	38	theme	digalactosylated	654:669	arg1	G2					671:672	digalactosylated G2	654:672	digalactosylated G2	654:672	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	8	39	theme	RA	1176:1177	arg1	n					1189:1189	n = 232	1189:1195	n = 232	1189:1195	To investigate this hypothesis, IgG glycosylation was characterized in serum samples from active RA patients (n = 232) and healthy controls (n = 232) by serum N-glycan analysis using the high performance liquid chromatography.
33402867	8	39	theme	RA	1176:1177	arg1	patients					1179:1186	active RA patients	1169:1186	active RA patients (n = 232)	1169:1196	To investigate this hypothesis, IgG glycosylation was characterized in serum samples from active RA patients (n = 232) and healthy controls (n = 232) by serum N-glycan analysis using the high performance liquid chromatography.
33402867	10	40	theme	hormone	1648:1654	arg1	estrogen					1656:1663	sex hormone estrogen	1644:1663	sex hormone estrogen via IgG glycosylation	1644:1685	In conclusion, IgG G0F-dependent inflammation differs in males and females, and these differences point to the differential regulation of inflammation by sex hormone estrogen via IgG glycosylation.
33402867	3	41	theme	arm	395:397	arg1	receptors					354:362	Fc gamma receptors	345:362	Fc gamma receptors	345:362	The Fc domain binds Fc gamma receptors and C1q proteins of the innate arm.
33402867	3	41	theme	arm	395:397	arg1	proteins					372:379	C1q proteins	368:379	C1q proteins	368:379	The Fc domain binds Fc gamma receptors and C1q proteins of the innate arm.
33402867	3	42	theme	C1q	368:370	arg1	proteins					372:379	C1q proteins	368:379	C1q proteins	368:379	The Fc domain binds Fc gamma receptors and C1q proteins of the innate arm.
33402867	7	43	theme	disease	1048:1054	arg1	Das28-CRP3					1066:1075	Das28-CRP3	1066:1075	Das28-CRP3	1066:1075	Here, it is hypothesized that the regulation of G0F by estrogen contributes to sex dichotomy in RA by setting up the level of IgG-dependent inflammation and therefore, RA disease activity (Das28-CRP3).
33402867	7	43	theme	disease	1048:1054	arg1	activity					1056:1063	RA disease activity	1045:1063	RA disease activity (Das28-CRP3)	1045:1076	Here, it is hypothesized that the regulation of G0F by estrogen contributes to sex dichotomy in RA by setting up the level of IgG-dependent inflammation and therefore, RA disease activity (Das28-CRP3).
33402867	8	44	theme	N-glycan	1238:1245	arg1	analysis					1247:1254	serum N-glycan analysis	1232:1254	serum N-glycan analysis using the high performance liquid chromatography	1232:1303	To investigate this hypothesis, IgG glycosylation was characterized in serum samples from active RA patients (n = 232) and healthy controls (n = 232) by serum N-glycan analysis using the high performance liquid chromatography.
33402867	7	45	theme	G0F	925:927	arg1	regulation					911:920	the regulation	907:920	the regulation of G0F by estrogen	907:939	Here, it is hypothesized that the regulation of G0F by estrogen contributes to sex dichotomy in RA by setting up the level of IgG-dependent inflammation and therefore, RA disease activity (Das28-CRP3).
33402867	8	46	theme	active	1169:1174	arg1	n					1189:1189	n = 232	1189:1195	n = 232	1189:1195	To investigate this hypothesis, IgG glycosylation was characterized in serum samples from active RA patients (n = 232) and healthy controls (n = 232) by serum N-glycan analysis using the high performance liquid chromatography.
33402867	8	46	theme	active	1169:1174	arg1	patients					1179:1186	active RA patients	1169:1186	active RA patients (n = 232)	1169:1196	To investigate this hypothesis, IgG glycosylation was characterized in serum samples from active RA patients (n = 232) and healthy controls (n = 232) by serum N-glycan analysis using the high performance liquid chromatography.
33402867	3	47	theme	gamma	348:352	arg1	receptors					354:362	Fc gamma receptors	345:362	Fc gamma receptors	345:362	The Fc domain binds Fc gamma receptors and C1q proteins of the innate arm.
33402867	0	48	theme	glycosylation	50:62	arg1	correlation					18:28	the correlation	14:28	the correlation of immunoglobulin G glycosylation with rheumatoid arthritis disease activity	14:105	Sex effect on the correlation of immunoglobulin G glycosylation with rheumatoid arthritis disease activity.
33402867	4	49	with	N-glycosylation	533:547	arg1	structures					563:572	variable structures	554:572	variable structures	554:572	Therefore, the IgG Fc domain serves as a bridge between the innate and adaptive arms and is regulated by an evolutionarily conserved N-glycosylation with variable structures.
33402867	9	50	theme	Fc	1340:1341	arg1	phenotype					1350:1358	the IgG Fc glycan phenotype	1332:1358	the IgG Fc glycan phenotype	1332:1358	According to the results, the IgG Fc glycan phenotype originates predominantly from the structure of G0F, and both G0F and G0FB correlate with Das28-CRP3 in females, but not in males.
33402867	0	51	theme	immunoglobulin	33:46	arg1	glycosylation					50:62	immunoglobulin G glycosylation	33:62	immunoglobulin G glycosylation with rheumatoid arthritis disease activity	33:105	Sex effect on the correlation of immunoglobulin G glycosylation with rheumatoid arthritis disease activity.
33402867	2	52	contain	has	287:289	arg2	Fc					313:314	Fc	313:314	Fc	313:314	Immunoglobulin G (IgG) produced by adaptive arm has 2 functional domains, Fc and Fab.
33402867	2	52	contain	has	287:289	arg1	IgG					257:259	IgG	257:259	IgG	257:259	Immunoglobulin G (IgG) produced by adaptive arm has 2 functional domains, Fc and Fab.
33402867	2	52	contain	has	287:289	arg1	G					254:254	Immunoglobulin G	239:254	Immunoglobulin G (IgG) produced by adaptive arm	239:285	Immunoglobulin G (IgG) produced by adaptive arm has 2 functional domains, Fc and Fab.
33402867	2	52	contain	has	287:289	arg2	Fab					320:322	Fab	320:322	Fab	320:322	Immunoglobulin G (IgG) produced by adaptive arm has 2 functional domains, Fc and Fab.
33402867	2	52	contain	has	287:289	arg2	domains					304:310	2 functional domains	291:310	2 functional domains	291:310	Immunoglobulin G (IgG) produced by adaptive arm has 2 functional domains, Fc and Fab.
33402867	7	53	theme	sex	956:958	arg1	dichotomy					960:968	sex dichotomy	956:968	sex dichotomy in RA	956:974	Here, it is hypothesized that the regulation of G0F by estrogen contributes to sex dichotomy in RA by setting up the level of IgG-dependent inflammation and therefore, RA disease activity (Das28-CRP3).
33402867	10	54	theme	inflammation	1628:1639	arg1	regulation					1614:1623	the differential regulation	1597:1623	the differential regulation of inflammation by sex hormone estrogen via IgG glycosylation	1597:1685	In conclusion, IgG G0F-dependent inflammation differs in males and females, and these differences point to the differential regulation of inflammation by sex hormone estrogen via IgG glycosylation.
33402867	9	55	theme	glycan	1343:1348	arg1	phenotype					1350:1358	the IgG Fc glycan phenotype	1332:1358	the IgG Fc glycan phenotype	1332:1358	According to the results, the IgG Fc glycan phenotype originates predominantly from the structure of G0F, and both G0F and G0FB correlate with Das28-CRP3 in females, but not in males.
33402867	4	56	theme	innate	460:465	arg1	arms					480:483	the innate and adaptive arms	456:483	the innate and adaptive arms	456:483	Therefore, the IgG Fc domain serves as a bridge between the innate and adaptive arms and is regulated by an evolutionarily conserved N-glycosylation with variable structures.
33402867	5	57	theme	monogalactosylated	627:644	arg1	G1					646:647	monogalactosylated G1	627:647	monogalactosylated G1	627:647	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	5	57	theme	monogalactosylated	627:644	arg1	glycans					581:587	These glycans	575:587	These glycans	575:587	These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F and G0FB.
33402867	8	58	theme	performance	1271:1281	arg1	chromatography					1290:1303	the high performance liquid chromatography	1262:1303	the high performance liquid chromatography	1262:1303	To investigate this hypothesis, IgG glycosylation was characterized in serum samples from active RA patients (n = 232) and healthy controls (n = 232) by serum N-glycan analysis using the high performance liquid chromatography.
33402867	10	59	theme	sex	1644:1646	arg1	estrogen					1656:1663	sex hormone estrogen	1644:1663	sex hormone estrogen via IgG glycosylation	1644:1685	In conclusion, IgG G0F-dependent inflammation differs in males and females, and these differences point to the differential regulation of inflammation by sex hormone estrogen via IgG glycosylation.
33402867	8	60	theme	=	1191:1191	arg1	n					1189:1189	n = 232	1189:1195	n = 232	1189:1195	To investigate this hypothesis, IgG glycosylation was characterized in serum samples from active RA patients (n = 232) and healthy controls (n = 232) by serum N-glycan analysis using the high performance liquid chromatography.
33402867	8	60	theme	=	1191:1191	arg1	patients					1179:1186	active RA patients	1169:1186	active RA patients (n = 232)	1169:1196	To investigate this hypothesis, IgG glycosylation was characterized in serum samples from active RA patients (n = 232) and healthy controls (n = 232) by serum N-glycan analysis using the high performance liquid chromatography.
33402867	10	61	theme	differential	1601:1612	arg1	regulation					1614:1623	the differential regulation	1597:1623	the differential regulation of inflammation by sex hormone estrogen via IgG glycosylation	1597:1685	In conclusion, IgG G0F-dependent inflammation differs in males and females, and these differences point to the differential regulation of inflammation by sex hormone estrogen via IgG glycosylation.
33402867	8	62	theme	high	1266:1269	arg1	chromatography					1290:1303	the high performance liquid chromatography	1262:1303	the high performance liquid chromatography	1262:1303	To investigate this hypothesis, IgG glycosylation was characterized in serum samples from active RA patients (n = 232) and healthy controls (n = 232) by serum N-glycan analysis using the high performance liquid chromatography.
33402867	2	63	theme	functional	293:302	arg1	Fab					320:322	Fab	320:322	Fab	320:322	Immunoglobulin G (IgG) produced by adaptive arm has 2 functional domains, Fc and Fab.
33402867	2	63	theme	functional	293:302	arg1	Fc					313:314	Fc	313:314	Fc	313:314	Immunoglobulin G (IgG) produced by adaptive arm has 2 functional domains, Fc and Fab.
33402867	2	63	theme	functional	293:302	arg1	domains					304:310	2 functional domains	291:310	2 functional domains	291:310	Immunoglobulin G (IgG) produced by adaptive arm has 2 functional domains, Fc and Fab.
33402867	4	64	theme	conserved	523:531	arg1	N-glycosylation					533:547	an evolutionarily conserved N-glycosylation	505:547	an evolutionarily conserved N-glycosylation with variable structures	505:572	Therefore, the IgG Fc domain serves as a bridge between the innate and adaptive arms and is regulated by an evolutionarily conserved N-glycosylation with variable structures.
33402867	9	65	theme	IgG	1336:1338	arg1	phenotype					1350:1358	the IgG Fc glycan phenotype	1332:1358	the IgG Fc glycan phenotype	1332:1358	According to the results, the IgG Fc glycan phenotype originates predominantly from the structure of G0F, and both G0F and G0FB correlate with Das28-CRP3 in females, but not in males.
33402867	2	66	theme	Immunoglobulin	239:252	arg1	IgG					257:259	IgG	257:259	IgG	257:259	Immunoglobulin G (IgG) produced by adaptive arm has 2 functional domains, Fc and Fab.
33402867	2	66	theme	Immunoglobulin	239:252	arg1	G					254:254	Immunoglobulin G	239:254	Immunoglobulin G (IgG) produced by adaptive arm	239:285	Immunoglobulin G (IgG) produced by adaptive arm has 2 functional domains, Fc and Fab.
33402867	10	67	theme	G0F-dependent	1509:1521	arg1	inflammation					1523:1534	IgG G0F-dependent inflammation	1505:1534	IgG G0F-dependent inflammation	1505:1534	In conclusion, IgG G0F-dependent inflammation differs in males and females, and these differences point to the differential regulation of inflammation by sex hormone estrogen via IgG glycosylation.
33402867	8	68	from	controls	1210:1217	arg1	samples					1156:1162	serum samples	1150:1162	serum samples from active RA patients (n = 232) and healthy controls (n = 232)	1150:1227	To investigate this hypothesis, IgG glycosylation was characterized in serum samples from active RA patients (n = 232) and healthy controls (n = 232) by serum N-glycan analysis using the high performance liquid chromatography.
33402867	4	69	theme	variable	554:561	arg1	structures					563:572	variable structures	554:572	variable structures	554:572	Therefore, the IgG Fc domain serves as a bridge between the innate and adaptive arms and is regulated by an evolutionarily conserved N-glycosylation with variable structures.
33402867	0	70	theme	G	48:48	arg1	glycosylation					50:62	immunoglobulin G glycosylation	33:62	immunoglobulin G glycosylation with rheumatoid arthritis disease activity	33:105	Sex effect on the correlation of immunoglobulin G glycosylation with rheumatoid arthritis disease activity.
33402867	8	71	theme	liquid	1283:1288	arg1	chromatography					1290:1303	the high performance liquid chromatography	1262:1303	the high performance liquid chromatography	1262:1303	To investigate this hypothesis, IgG glycosylation was characterized in serum samples from active RA patients (n = 232) and healthy controls (n = 232) by serum N-glycan analysis using the high performance liquid chromatography.
33402867	9	72	theme	G0F	1407:1409	arg1	structure					1394:1402	the structure	1390:1402	the structure of G0F	1390:1409	According to the results, the IgG Fc glycan phenotype originates predominantly from the structure of G0F, and both G0F and G0FB correlate with Das28-CRP3 in females, but not in males.
33402867	1	73	theme	autoantibodies	223:236	arg1	presence					211:218	a presence	209:218	a presence of autoantibodies	209:236	Rheumatoid arthritis (RA) is a chronic autoimmune disease which affects females more than males with a presence of autoantibodies.
31981886	0	0	theme	alternative	63:73	arg1	ingredients					75:85	alternative ingredients	63:85	alternative ingredients in gluten-free crackers	63:109	Mucilage and cladode flour from cactus (Opuntia monacantha) as alternative ingredients in gluten-free crackers.
31981886	2	1	theme	%	473:473	arg1	CF					475:476	15% CF	471:476	15% CF	471:476	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	5	2	theme	physical	738:745	arg1	properties					747:756	crackers' physical properties	728:756	crackers' physical properties	728:756	In general, crackers' physical properties were not impacted by CM or CF addition, except for the darker color of CF-supplemented crackers.
31981886	9	3	theme	gluten-free	1142:1152	arg1	formulation					1162:1172	gluten-free cracker formulation	1142:1172	gluten-free cracker formulation	1142:1172	Therefore, CM and CF are interesting alternatives for gluten-free cracker formulation.
31981886	2	4	theme	15	471:472	arg1	%					473:473	%	473:473	%	473:473	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	2	5	theme	gums	513:516	arg1	free					494:497	free	494:497	free	494:497	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	6	6	theme	antioxidant	927:937	arg1	activity					939:946	antioxidant activity	927:946	antioxidant activity	927:946	The C-CM and CF-incorporated crackers showed higher total phenolics and antioxidant activity.
31981886	1	7	theme	gluten-free	223:233	arg1	snacks					235:240	gluten-free snacks	223:240	gluten-free snacks	223:240	Cactus cladode offers innovative ingredients such as cactus mucilage (CM) and cladode flour (CF) for producing gluten-free snacks.
31981886	8	8	theme	Sensory	1014:1020	arg1	evaluation					1022:1031	Sensory evaluation	1014:1031	Sensory evaluation	1014:1031	Sensory evaluation revealed that C-CM and C-CF5 were those most accepted.
31981886	9	9	theme	cracker	1154:1160	arg1	formulation					1162:1172	gluten-free cracker formulation	1142:1172	gluten-free cracker formulation	1142:1172	Therefore, CM and CF are interesting alternatives for gluten-free cracker formulation.
31981886	2	10	theme	commercial	502:511	arg1	gums					513:516	commercial gums	502:516	commercial gums	502:516	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	3	11	theme	great	577:581	arg1	potentiality					597:608	its great technological potentiality	573:608	its great technological potentiality	573:608	CF characterization is also presented, demonstrating its great technological potentiality.
31981886	2	12	dep	C-C	284:286	arg1	cracker					297:303	control cracker	289:303	control cracker prepared with 2% of commercial gums	289:339	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	3	13	theme	technological	583:595	arg1	potentiality					597:608	its great technological potentiality	573:608	its great technological potentiality	573:608	CF characterization is also presented, demonstrating its great technological potentiality.
31981886	2	14	theme	free	494:497	arg1	crackers					433:440	crackers	433:440	crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums	433:516	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	2	15	theme	different	248:256	arg1	crackers					258:265	Five different crackers	243:265	Five different crackers	243:265	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	1	16	theme	cactus	165:170	arg1	CM					182:183	CM	182:183	CM	182:183	Cactus cladode offers innovative ingredients such as cactus mucilage (CM) and cladode flour (CF) for producing gluten-free snacks.
31981886	1	16	theme	cactus	165:170	arg1	mucilage					172:179	cactus mucilage	165:179	cactus mucilage (CM)	165:184	Cactus cladode offers innovative ingredients such as cactus mucilage (CM) and cladode flour (CF) for producing gluten-free snacks.
31981886	0	17	theme	gluten-free	90:100	arg1	crackers					102:109	gluten-free crackers	90:109	gluten-free crackers	90:109	Mucilage and cladode flour from cactus (Opuntia monacantha) as alternative ingredients in gluten-free crackers.
31981886	0	18	from	cactus	32:37	arg1	flour					21:25	flour	21:25	flour	21:25	Mucilage and cladode flour from cactus (Opuntia monacantha) as alternative ingredients in gluten-free crackers.
31981886	4	19	theme	C-CM	640:643	arg1	composition					625:635	The proximate composition	611:635	The proximate composition of C-CM and C-CF	611:652	The proximate composition of C-CM and C-CF mostly differed for ash and fiber content as compared to C-C.
31981886	6	20	theme	total	907:911	arg1	phenolics					913:921	higher total phenolics	900:921	higher total phenolics	900:921	The C-CM and CF-incorporated crackers showed higher total phenolics and antioxidant activity.
31981886	2	21	theme	CM	374:375	arg1	gums					399:402	CM instead of commercial gums	374:402	CM instead of commercial gums	374:402	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	9	22	theme	interesting	1113:1123	arg1	CF					1106:1107	CF	1106:1107	CF	1106:1107	Therefore, CM and CF are interesting alternatives for gluten-free cracker formulation.
31981886	9	22	theme	interesting	1113:1123	arg1	alternatives					1125:1136	interesting alternatives	1113:1136	interesting alternatives for gluten-free cracker formulation	1113:1172	Therefore, CM and CF are interesting alternatives for gluten-free cracker formulation.
31981886	9	22	theme	interesting	1113:1123	arg1	CM					1099:1100	CM	1099:1100	CM	1099:1100	Therefore, CM and CF are interesting alternatives for gluten-free cracker formulation.
31981886	2	23	theme	gums	336:339	arg1	%					320:320	2%	319:320	2% of commercial gums	319:339	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	2	23	theme	gums	336:339	arg1	gums					336:339	commercial gums	325:339	commercial gums	325:339	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	7	24	theme	latter	967:972	arg1	samples					974:980	the latter samples	963:980	the latter samples	963:980	Additionally, the latter samples were enhanced with carotenoids.
31981886	4	25	theme	proximate	615:623	arg1	composition					625:635	The proximate composition	611:635	The proximate composition of C-CM and C-CF	611:652	The proximate composition of C-CM and C-CF mostly differed for ash and fiber content as compared to C-C.
31981886	1	26	theme	innovative	134:143	arg1	ingredients					145:155	innovative ingredients	134:155	innovative ingredients such as cactus mucilage (CM) and cladode flour (CF) for producing gluten-free snacks	134:240	Cactus cladode offers innovative ingredients such as cactus mucilage (CM) and cladode flour (CF) for producing gluten-free snacks.
31981886	1	26	theme	innovative	134:143	arg1	mucilage					172:179	cactus mucilage	165:179	cactus mucilage (CM)	165:184	Cactus cladode offers innovative ingredients such as cactus mucilage (CM) and cladode flour (CF) for producing gluten-free snacks.
31981886	1	26	theme	innovative	134:143	arg1	flour					198:202	cladode flour	190:202	cladode flour (CF)	190:207	Cactus cladode offers innovative ingredients such as cactus mucilage (CM) and cladode flour (CF) for producing gluten-free snacks.
31981886	2	27	dep	formulated	272:281	arg1	C-CF15					425:430	C-CF15	425:430	C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums)	425:517	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	2	27	dep	formulated	272:281	arg1	C-CM					343:346	C-CM	343:346	C-CM (cracker containing 2% of CM instead of commercial gums)	343:403	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	2	27	dep	formulated	272:281	arg1	C-C					284:286	C-C	284:286	C-C (control cracker prepared with 2% of commercial gums)	284:340	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	2	27	dep	formulated	272:281	arg1	C-CF5					406:410	C-CF5	406:410	C-CF5	406:410	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	2	27	dep	formulated	272:281	arg1	C-CF10					413:418	C-CF10	413:418	C-CF10	413:418	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	6	28	theme	CF-incorporated	868:882	arg1	crackers					884:891	The C-CM and CF-incorporated crackers	855:891	The C-CM and CF-incorporated crackers	855:891	The C-CM and CF-incorporated crackers showed higher total phenolics and antioxidant activity.
31981886	5	29	theme	crackers	845:852	arg1	color					820:824	the darker color	809:824	the darker color of CF-supplemented crackers	809:852	In general, crackers' physical properties were not impacted by CM or CF addition, except for the darker color of CF-supplemented crackers.
31981886	6	30	theme	higher	900:905	arg1	phenolics					913:921	higher total phenolics	900:921	higher total phenolics	900:921	The C-CM and CF-incorporated crackers showed higher total phenolics and antioxidant activity.
31981886	1	31	theme	cladode	190:196	arg1	CF					205:206	CF	205:206	CF	205:206	Cactus cladode offers innovative ingredients such as cactus mucilage (CM) and cladode flour (CF) for producing gluten-free snacks.
31981886	1	31	theme	cladode	190:196	arg1	flour					198:202	cladode flour	190:202	cladode flour (CF)	190:207	Cactus cladode offers innovative ingredients such as cactus mucilage (CM) and cladode flour (CF) for producing gluten-free snacks.
31981886	2	32	theme	gums	399:402	arg1	%					369:369	2%	368:369	2% of CM instead of commercial gums	368:402	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	2	32	theme	gums	399:402	arg1	gums					399:402	CM instead of commercial gums	374:402	CM instead of commercial gums	374:402	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	2	33	dep	C-CF15	425:430	arg1	crackers					433:440	crackers	433:440	crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums	433:516	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	2	34	theme	commercial	388:397	arg1	gums					399:402	CM instead of commercial gums	374:402	CM instead of commercial gums	374:402	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	0	35	dep	cactus	32:37	arg1	monacantha					48:57	Opuntia monacantha	40:57	Opuntia monacantha	40:57	Mucilage and cladode flour from cactus (Opuntia monacantha) as alternative ingredients in gluten-free crackers.
31981886	3	36	theme	CF	520:521	arg1	characterization					523:538	CF characterization	520:538	CF characterization	520:538	CF characterization is also presented, demonstrating its great technological potentiality.
31981886	4	37	theme	fiber	682:686	arg1	content					688:694	fiber content	682:694	fiber content	682:694	The proximate composition of C-CM and C-CF mostly differed for ash and fiber content as compared to C-C.
31981886	1	38	theme	Cactus	112:117	arg1	cladode					119:125	Cactus cladode	112:125	Cactus cladode	112:125	Cactus cladode offers innovative ingredients such as cactus mucilage (CM) and cladode flour (CF) for producing gluten-free snacks.
31981886	5	39	theme	darker	813:818	arg1	color					820:824	the darker color	809:824	the darker color of CF-supplemented crackers	809:852	In general, crackers' physical properties were not impacted by CM or CF addition, except for the darker color of CF-supplemented crackers.
31981886	5	40	theme	CF	785:786	arg1	addition					788:795	CF addition	785:795	CF addition	785:795	In general, crackers' physical properties were not impacted by CM or CF addition, except for the darker color of CF-supplemented crackers.
31981886	6	41	theme	C-CM	859:862	arg1	crackers					884:891	The C-CM and CF-incorporated crackers	855:891	The C-CM and CF-incorporated crackers	855:891	The C-CM and CF-incorporated crackers showed higher total phenolics and antioxidant activity.
31981886	2	42	contain	containing	357:366	arg1	cracker					349:355	cracker	349:355	cracker containing 2% of CM instead of commercial gums	349:402	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	2	42	contain	containing	357:366	arg2	gums					399:402	CM instead of commercial gums	374:402	CM instead of commercial gums	374:402	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	2	42	contain	containing	357:366	arg2	%					369:369	2%	368:369	2% of CM instead of commercial gums	368:402	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	4	43	theme	C-CF	649:652	arg1	composition					625:635	The proximate composition	611:635	The proximate composition of C-CM and C-CF	611:652	The proximate composition of C-CM and C-CF mostly differed for ash and fiber content as compared to C-C.
31981886	2	44	theme	control	289:295	arg1	cracker					297:303	control cracker	289:303	control cracker prepared with 2% of commercial gums	289:339	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	0	45	from	ingredients	75:85	arg1	crackers					102:109	gluten-free crackers	90:109	gluten-free crackers	90:109	Mucilage and cladode flour from cactus (Opuntia monacantha) as alternative ingredients in gluten-free crackers.
31981886	2	46	dep	C-CM	343:346	arg1	cracker					349:355	cracker	349:355	cracker containing 2% of CM instead of commercial gums	349:402	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	2	47	theme	commercial	325:334	arg1	gums					336:339	commercial gums	325:339	commercial gums	325:339	Five different crackers were formulated: C-C (control cracker prepared with 2% of commercial gums), C-CM (cracker containing 2% of CM instead of commercial gums), C-CF5, C-CF10, and C-CF15 (crackers incorporated of 5%, 10%, and 15% CF, respectively - free of commercial gums).
31981886	5	48	theme	CF-supplemented	829:843	arg1	crackers					845:852	CF-supplemented crackers	829:852	CF-supplemented crackers	829:852	In general, crackers' physical properties were not impacted by CM or CF addition, except for the darker color of CF-supplemented crackers.
32950898	0	0	theme	Andean	74:79	arg1	genotypes					86:94	Andean bean genotypes	74:94	Andean bean genotypes	74:94	The influence of chemical composition diversity in the cooking quality of Andean bean genotypes.
32950898	5	1	theme	starch	652:657	arg1	content					659:665	mainly starch content	645:665	mainly starch content (39.43 g 100 g-1, BRS Realce to 51.92 g 100 g-1, LP15-04)	645:723	Andean beans showed variability in chemical composition, mainly starch content (39.43 g 100 g-1, BRS Realce to 51.92 g 100 g-1, LP15-04) and polymer composition.
32950898	2	2	theme	genotypes	335:343	arg1	quality					308:314	the cooking quality	296:314	the cooking quality of 14 Andean beans genotypes with different seed coat colors	296:375	The objective of this study was to evaluate the influence of chemical composition on the cooking quality of 14 Andean beans genotypes with different seed coat colors.
32950898	5	3	dep	content	659:665	arg1	100 g-1					676:682	39.43 g 100 g-1	668:682	39.43 g 100 g-1	668:682	Andean beans showed variability in chemical composition, mainly starch content (39.43 g 100 g-1, BRS Realce to 51.92 g 100 g-1, LP15-04) and polymer composition.
32950898	5	3	dep	content	659:665	arg1	Realce					689:694	BRS Realce	685:694	BRS Realce to 51.92 g 100 g-1	685:713	Andean beans showed variability in chemical composition, mainly starch content (39.43 g 100 g-1, BRS Realce to 51.92 g 100 g-1, LP15-04) and polymer composition.
32950898	5	3	dep	content	659:665	arg1	LP15-04					716:722	LP15-04	716:722	LP15-04	716:722	Andean beans showed variability in chemical composition, mainly starch content (39.43 g 100 g-1, BRS Realce to 51.92 g 100 g-1, LP15-04) and polymer composition.
32950898	0	4	from	influence	4:12	arg1	quality					63:69	the cooking quality	51:69	the cooking quality of Andean bean genotypes	51:94	The influence of chemical composition diversity in the cooking quality of Andean bean genotypes.
32950898	2	5	theme	beans	329:333	arg1	genotypes					335:343	14 Andean beans genotypes	319:343	14 Andean beans genotypes with different seed coat colors	319:375	The objective of this study was to evaluate the influence of chemical composition on the cooking quality of 14 Andean beans genotypes with different seed coat colors.
32950898	6	6	theme	individual	871:880	arg1	compounds					882:890	the individual compounds	867:890	the individual compounds	867:890	The profile of starch and interactions among minerals and chemical compounds influenced the cooking profiles than do the individual compounds.
32950898	0	7	theme	genotypes	86:94	arg1	quality					63:69	the cooking quality	51:69	the cooking quality of Andean bean genotypes	51:94	The influence of chemical composition diversity in the cooking quality of Andean bean genotypes.
32950898	2	8	theme	chemical	272:279	arg1	composition					281:291	chemical composition	272:291	chemical composition	272:291	The objective of this study was to evaluate the influence of chemical composition on the cooking quality of 14 Andean beans genotypes with different seed coat colors.
32950898	2	9	theme	composition	281:291	arg1	influence					259:267	the influence	255:267	the influence of chemical composition on the cooking quality of 14 Andean beans genotypes with different seed coat colors	255:375	The objective of this study was to evaluate the influence of chemical composition on the cooking quality of 14 Andean beans genotypes with different seed coat colors.
32950898	0	10	theme	bean	81:84	arg1	genotypes					86:94	Andean bean genotypes	74:94	Andean bean genotypes	74:94	The influence of chemical composition diversity in the cooking quality of Andean bean genotypes.
32950898	6	11	theme	starch	765:770	arg1	profile					754:760	The profile	750:760	The profile of starch and interactions among minerals and chemical compounds	750:825	The profile of starch and interactions among minerals and chemical compounds influenced the cooking profiles than do the individual compounds.
32950898	4	12	theme	%	514:514	arg1	%					524:524	128.4% to 160.7%	509:524	128.4% to 160.7%	509:524	WR values ranged from 128.4% to 160.7% and CT ranged from 13.7 (BRS Embaixador) to 21.7 min (KID44).
32950898	5	13	theme	chemical	623:630	arg1	composition					632:642	chemical composition	623:642	chemical composition	623:642	Andean beans showed variability in chemical composition, mainly starch content (39.43 g 100 g-1, BRS Realce to 51.92 g 100 g-1, LP15-04) and polymer composition.
32950898	7	14	theme	chemical	961:968	arg1	composition					970:980	their chemical composition	955:980	their chemical composition	955:980	Andean beans traits of cooking, mainly CT, were influenced by their chemical composition; however they can be incorporated into diets without drastic changes in preparation methods.
32950898	1	15	dep	beans	104:108	arg1	compositions					140:151	chemical compositions	131:151	chemical compositions	131:151	Andean beans (Phaseolus vulgaris) chemical compositions and cooking characteristics contribute to a healthy diet.
32950898	1	16	theme	cooking	157:163	arg1	characteristics					165:179	cooking characteristics	157:179	cooking characteristics	157:179	Andean beans (Phaseolus vulgaris) chemical compositions and cooking characteristics contribute to a healthy diet.
32950898	7	17	from	changes	1043:1049	arg1	methods					1066:1072	preparation methods	1054:1072	preparation methods	1054:1072	Andean beans traits of cooking, mainly CT, were influenced by their chemical composition; however they can be incorporated into diets without drastic changes in preparation methods.
32950898	6	18	dep	do	864:865	arg1	than					859:862	than	859:862	than	859:862	The profile of starch and interactions among minerals and chemical compounds influenced the cooking profiles than do the individual compounds.
32950898	5	19	theme	polymer	729:735	arg1	composition					737:747	polymer composition	729:747	polymer composition	729:747	Andean beans showed variability in chemical composition, mainly starch content (39.43 g 100 g-1, BRS Realce to 51.92 g 100 g-1, LP15-04) and polymer composition.
32950898	5	20	theme	BRS	685:687	arg1	100 g-1					676:682	39.43 g 100 g-1	668:682	39.43 g 100 g-1	668:682	Andean beans showed variability in chemical composition, mainly starch content (39.43 g 100 g-1, BRS Realce to 51.92 g 100 g-1, LP15-04) and polymer composition.
32950898	5	20	theme	BRS	685:687	arg1	Realce					689:694	BRS Realce	685:694	BRS Realce to 51.92 g 100 g-1	685:713	Andean beans showed variability in chemical composition, mainly starch content (39.43 g 100 g-1, BRS Realce to 51.92 g 100 g-1, LP15-04) and polymer composition.
32950898	2	21	theme	cooking	300:306	arg1	quality					308:314	the cooking quality	296:314	the cooking quality of 14 Andean beans genotypes with different seed coat colors	296:375	The objective of this study was to evaluate the influence of chemical composition on the cooking quality of 14 Andean beans genotypes with different seed coat colors.
32950898	2	22	theme	coat	365:368	arg1	colors					370:375	different seed coat colors	350:375	different seed coat colors	350:375	The objective of this study was to evaluate the influence of chemical composition on the cooking quality of 14 Andean beans genotypes with different seed coat colors.
32950898	6	23	theme	interactions	776:787	arg1	profile					754:760	The profile	750:760	The profile of starch and interactions among minerals and chemical compounds	750:825	The profile of starch and interactions among minerals and chemical compounds influenced the cooking profiles than do the individual compounds.
32950898	4	24	theme	WR	487:488	arg1	values					490:495	WR values	487:495	WR values ranged from 128.4% to 160.7% and CT	487:531	WR values ranged from 128.4% to 160.7% and CT ranged from 13.7 (BRS Embaixador) to 21.7 min (KID44).
32950898	2	25	theme	seed	360:363	arg1	colors					370:375	different seed coat colors	350:375	different seed coat colors	350:375	The objective of this study was to evaluate the influence of chemical composition on the cooking quality of 14 Andean beans genotypes with different seed coat colors.
32950898	0	26	theme	chemical	17:24	arg1	diversity					38:46	chemical composition diversity	17:46	chemical composition diversity	17:46	The influence of chemical composition diversity in the cooking quality of Andean bean genotypes.
32950898	5	27	theme	51.92 g	699:705	arg1	100 g-1					707:713	51.92 g 100 g-1	699:713	51.92 g 100 g-1	699:713	Andean beans showed variability in chemical composition, mainly starch content (39.43 g 100 g-1, BRS Realce to 51.92 g 100 g-1, LP15-04) and polymer composition.
32950898	6	28	theme	cooking	842:848	arg1	profiles					850:857	the cooking profiles	838:857	the cooking profiles than do the individual compounds	838:890	The profile of starch and interactions among minerals and chemical compounds influenced the cooking profiles than do the individual compounds.
32950898	5	29	theme	Andean	588:593	arg1	beans					595:599	Andean beans	588:599	Andean beans	588:599	Andean beans showed variability in chemical composition, mainly starch content (39.43 g 100 g-1, BRS Realce to 51.92 g 100 g-1, LP15-04) and polymer composition.
32950898	1	30	theme	Andean	97:102	arg1	beans					104:108	Andean beans	97:108	Andean beans (Phaseolus vulgaris)	97:129	Andean beans (Phaseolus vulgaris) chemical compositions and cooking characteristics contribute to a healthy diet.
32950898	1	30	theme	Andean	97:102	arg1	vulgaris					121:128	Phaseolus vulgaris	111:128	Phaseolus vulgaris	111:128	Andean beans (Phaseolus vulgaris) chemical compositions and cooking characteristics contribute to a healthy diet.
32950898	1	31	theme	healthy	197:203	arg1	diet					205:208	a healthy diet	195:208	a healthy diet	195:208	Andean beans (Phaseolus vulgaris) chemical compositions and cooking characteristics contribute to a healthy diet.
32950898	0	32	theme	diversity	38:46	arg1	influence					4:12	The influence	0:12	The influence of chemical composition diversity in the cooking quality of Andean bean genotypes.	0:95	The influence of chemical composition diversity in the cooking quality of Andean bean genotypes.
32950898	0	33	theme	composition	26:36	arg1	diversity					38:46	chemical composition diversity	17:46	chemical composition diversity	17:46	The influence of chemical composition diversity in the cooking quality of Andean bean genotypes.
32950898	3	34	theme	water	397:401	arg1	WR					414:415	WR	414:415	WR	414:415	More specifically, water retention (WR), cooking time (CT), and solids released in the broth, were analysed.
32950898	3	34	theme	water	397:401	arg1	retention					403:411	water retention	397:411	water retention (WR)	397:416	More specifically, water retention (WR), cooking time (CT), and solids released in the broth, were analysed.
32950898	5	35	from	variability	608:618	arg1	composition					632:642	chemical composition	623:642	chemical composition	623:642	Andean beans showed variability in chemical composition, mainly starch content (39.43 g 100 g-1, BRS Realce to 51.92 g 100 g-1, LP15-04) and polymer composition.
32950898	5	35	from	variability	608:618	arg1	composition					737:747	polymer composition	729:747	polymer composition	729:747	Andean beans showed variability in chemical composition, mainly starch content (39.43 g 100 g-1, BRS Realce to 51.92 g 100 g-1, LP15-04) and polymer composition.
32950898	5	35	from	variability	608:618	arg1	content					659:665	mainly starch content	645:665	mainly starch content (39.43 g 100 g-1, BRS Realce to 51.92 g 100 g-1, LP15-04)	645:723	Andean beans showed variability in chemical composition, mainly starch content (39.43 g 100 g-1, BRS Realce to 51.92 g 100 g-1, LP15-04) and polymer composition.
32950898	4	36	dep	13.7	545:548	arg1	21.7 min					570:577	21.7 min (KID44)	570:585	13.7 (BRS Embaixador) to 21.7 min (KID44)	545:585	WR values ranged from 128.4% to 160.7% and CT ranged from 13.7 (BRS Embaixador) to 21.7 min (KID44).
32950898	4	36	dep	13.7	545:548	arg1	to					567:568	to	567:568	to	567:568	WR values ranged from 128.4% to 160.7% and CT ranged from 13.7 (BRS Embaixador) to 21.7 min (KID44).
32950898	7	37	theme	beans	900:904	arg1	CT					932:933	CT	932:933	mainly CT	925:933	Andean beans traits of cooking, mainly CT, were influenced by their chemical composition; however they can be incorporated into diets without drastic changes in preparation methods.
32950898	7	37	theme	beans	900:904	arg1	traits					906:911	Andean beans traits	893:911	Andean beans traits of cooking	893:922	Andean beans traits of cooking, mainly CT, were influenced by their chemical composition; however they can be incorporated into diets without drastic changes in preparation methods.
32950898	3	38	theme	cooking	419:425	arg1	CT					433:434	CT	433:434	CT	433:434	More specifically, water retention (WR), cooking time (CT), and solids released in the broth, were analysed.
32950898	3	38	theme	cooking	419:425	arg1	time					427:430	cooking time	419:430	cooking time (CT)	419:435	More specifically, water retention (WR), cooking time (CT), and solids released in the broth, were analysed.
32950898	2	39	theme	Andean	322:327	arg1	genotypes					335:343	14 Andean beans genotypes	319:343	14 Andean beans genotypes with different seed coat colors	319:375	The objective of this study was to evaluate the influence of chemical composition on the cooking quality of 14 Andean beans genotypes with different seed coat colors.
32950898	6	40	theme	chemical	808:815	arg1	compounds					817:825	chemical compounds	808:825	chemical compounds	808:825	The profile of starch and interactions among minerals and chemical compounds influenced the cooking profiles than do the individual compounds.
32950898	2	41	with	genotypes	335:343	arg1	colors					370:375	different seed coat colors	350:375	different seed coat colors	350:375	The objective of this study was to evaluate the influence of chemical composition on the cooking quality of 14 Andean beans genotypes with different seed coat colors.
32950898	7	42	theme	drastic	1035:1041	arg1	changes					1043:1049	drastic changes	1035:1049	drastic changes in preparation methods	1035:1072	Andean beans traits of cooking, mainly CT, were influenced by their chemical composition; however they can be incorporated into diets without drastic changes in preparation methods.
32950898	2	43	from	influence	259:267	arg1	quality					308:314	the cooking quality	296:314	the cooking quality of 14 Andean beans genotypes with different seed coat colors	296:375	The objective of this study was to evaluate the influence of chemical composition on the cooking quality of 14 Andean beans genotypes with different seed coat colors.
32950898	4	44	dep	BRS	551:553	arg1	Embaixador					555:564	Embaixador	555:564	BRS Embaixador	551:564	WR values ranged from 128.4% to 160.7% and CT ranged from 13.7 (BRS Embaixador) to 21.7 min (KID44).
32950898	5	45	theme	39.43 g	668:674	arg1	100 g-1					676:682	39.43 g 100 g-1	668:682	39.43 g 100 g-1	668:682	Andean beans showed variability in chemical composition, mainly starch content (39.43 g 100 g-1, BRS Realce to 51.92 g 100 g-1, LP15-04) and polymer composition.
32950898	5	45	theme	39.43 g	668:674	arg1	Realce					689:694	BRS Realce	685:694	BRS Realce to 51.92 g 100 g-1	685:713	Andean beans showed variability in chemical composition, mainly starch content (39.43 g 100 g-1, BRS Realce to 51.92 g 100 g-1, LP15-04) and polymer composition.
32950898	5	45	theme	39.43 g	668:674	arg1	LP15-04					716:722	LP15-04	716:722	LP15-04	716:722	Andean beans showed variability in chemical composition, mainly starch content (39.43 g 100 g-1, BRS Realce to 51.92 g 100 g-1, LP15-04) and polymer composition.
32950898	7	46	theme	Andean	893:898	arg1	CT					932:933	CT	932:933	mainly CT	925:933	Andean beans traits of cooking, mainly CT, were influenced by their chemical composition; however they can be incorporated into diets without drastic changes in preparation methods.
32950898	7	46	theme	Andean	893:898	arg1	traits					906:911	Andean beans traits	893:911	Andean beans traits of cooking	893:922	Andean beans traits of cooking, mainly CT, were influenced by their chemical composition; however they can be incorporated into diets without drastic changes in preparation methods.
32950898	1	47	theme	Phaseolus	111:119	arg1	beans					104:108	Andean beans	97:108	Andean beans (Phaseolus vulgaris)	97:129	Andean beans (Phaseolus vulgaris) chemical compositions and cooking characteristics contribute to a healthy diet.
32950898	1	47	theme	Phaseolus	111:119	arg1	vulgaris					121:128	Phaseolus vulgaris	111:128	Phaseolus vulgaris	111:128	Andean beans (Phaseolus vulgaris) chemical compositions and cooking characteristics contribute to a healthy diet.
32950898	7	48	theme	cooking	916:922	arg1	CT					932:933	CT	932:933	mainly CT	925:933	Andean beans traits of cooking, mainly CT, were influenced by their chemical composition; however they can be incorporated into diets without drastic changes in preparation methods.
32950898	7	48	theme	cooking	916:922	arg1	traits					906:911	Andean beans traits	893:911	Andean beans traits of cooking	893:922	Andean beans traits of cooking, mainly CT, were influenced by their chemical composition; however they can be incorporated into diets without drastic changes in preparation methods.
32950898	4	49	dep	%	524:524	arg1	to					516:517	to	516:517	to	516:517	WR values ranged from 128.4% to 160.7% and CT ranged from 13.7 (BRS Embaixador) to 21.7 min (KID44).
32950898	6	50	dep	profiles	850:857	arg1	do					864:865	do	864:865	do	864:865	The profile of starch and interactions among minerals and chemical compounds influenced the cooking profiles than do the individual compounds.
32950898	2	51	theme	different	350:358	arg1	colors					370:375	different seed coat colors	350:375	different seed coat colors	350:375	The objective of this study was to evaluate the influence of chemical composition on the cooking quality of 14 Andean beans genotypes with different seed coat colors.
32950898	0	52	theme	cooking	55:61	arg1	quality					63:69	the cooking quality	51:69	the cooking quality of Andean bean genotypes	51:94	The influence of chemical composition diversity in the cooking quality of Andean bean genotypes.
32950898	7	53	theme	preparation	1054:1064	arg1	methods					1066:1072	preparation methods	1054:1072	preparation methods	1054:1072	Andean beans traits of cooking, mainly CT, were influenced by their chemical composition; however they can be incorporated into diets without drastic changes in preparation methods.
32950898	2	54	theme	study	233:237	arg1	objective					215:223	The objective	211:223	The objective of this study	211:237	The objective of this study was to evaluate the influence of chemical composition on the cooking quality of 14 Andean beans genotypes with different seed coat colors.
32950898	1	55	theme	chemical	131:138	arg1	compositions					140:151	chemical compositions	131:151	chemical compositions	131:151	Andean beans (Phaseolus vulgaris) chemical compositions and cooking characteristics contribute to a healthy diet.
32894594	9	0	theme	biofilm	1611:1617	arg1	phenotypes					1619:1628	biofilm phenotypes	1611:1628	biofilm phenotypes with power in the direct comparison of these strains	1611:1681	We describe the influence of curli, cellulose, and the pEtN modification on biofilm phenotypes with power in the direct comparison of these strains.
32894594	7	1	theme	assembly	1275:1282	arg1	principles					1284:1293	matrix assembly principles	1268:1293	matrix assembly principles	1268:1293	The ECM isolated from AR3110 contains approximately twice the amount of pEtN cellulose relative to curli content as UTI89, revealing plasticity in matrix assembly principles among strains.
32894594	9	2	with	phenotypes	1619:1628	arg1	power					1635:1639	power	1635:1639	power	1635:1639	We describe the influence of curli, cellulose, and the pEtN modification on biofilm phenotypes with power in the direct comparison of these strains.
32894594	2	3	theme	curli	290:294	arg1	fibers					304:309	curli amyloid fibers	290:309	curli amyloid fibers	290:309	Escherichia coli direct the assembly of two insoluble biopolymers, curli amyloid fibers, and phosphoethanolamine (pEtN) cellulose, to build remarkable biofilm architectures.
32894594	2	4	theme	biopolymers	277:287	arg1	assembly					251:258	the assembly	247:258	the assembly of two insoluble biopolymers, curli amyloid fibers, and phosphoethanolamine (pEtN) cellulose	247:351	Escherichia coli direct the assembly of two insoluble biopolymers, curli amyloid fibers, and phosphoethanolamine (pEtN) cellulose, to build remarkable biofilm architectures.
32894594	1	5	theme	self-produced	181:193	arg1	ECM					217:219	ECM	217:219	ECM	217:219	Bacterial biofilms are communities of bacteria entangled in a self-produced extracellular matrix (ECM).
32894594	1	5	theme	self-produced	181:193	arg1	matrix					209:214	a self-produced extracellular matrix	179:214	a self-produced extracellular matrix (ECM)	179:220	Bacterial biofilms are communities of bacteria entangled in a self-produced extracellular matrix (ECM).
32894594	9	6	theme	direct	1648:1653	arg1	comparison					1655:1664	the direct comparison	1644:1664	the direct comparison of these strains	1644:1681	We describe the influence of curli, cellulose, and the pEtN modification on biofilm phenotypes with power in the direct comparison of these strains.
32894594	4	7	theme	ECM	726:728	arg1	correlates					740:749	quantitative ECM molecular correlates	713:749	quantitative ECM molecular correlates	713:749	Defining ECM composition involving insoluble polymeric assemblies poses unique challenges to analysis and, thus, to comparing strains with quantitative ECM molecular correlates.
32894594	5	8	theme	isolated	919:926	arg1	ECM					928:930	the isolated ECM	915:930	the isolated ECM of the E. coli laboratory K12 strain, AR3110	915:975	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	8	9	theme	biofilm	1397:1403	arg1	models					1405:1410	three biofilm models	1391:1410	three biofilm models	1391:1410	The two parent strains and a panel of relevant gene mutants were investigated in three biofilm models, examining: (a) macrocolonies on agar, (b) pellicles at the liquid-air interface, and (c) biomass accumulation on plastic.
32894594	8	9	theme	biofilm	1397:1403	arg1	examining					1413:1421	examining	1413:1421	examining	1413:1421	The two parent strains and a panel of relevant gene mutants were investigated in three biofilm models, examining: (a) macrocolonies on agar, (b) pellicles at the liquid-air interface, and (c) biomass accumulation on plastic.
32894594	2	10	theme	fibers	304:309	arg1	assembly					251:258	the assembly	247:258	the assembly of two insoluble biopolymers, curli amyloid fibers, and phosphoethanolamine (pEtN) cellulose	247:351	Escherichia coli direct the assembly of two insoluble biopolymers, curli amyloid fibers, and phosphoethanolamine (pEtN) cellulose, to build remarkable biofilm architectures.
32894594	9	11	theme	strains	1675:1681	arg1	comparison					1655:1664	the direct comparison	1644:1664	the direct comparison of these strains	1644:1681	We describe the influence of curli, cellulose, and the pEtN modification on biofilm phenotypes with power in the direct comparison of these strains.
32894594	4	12	theme	comparing	690:698	arg1	strains					700:706	comparing strains	690:706	comparing strains with quantitative ECM molecular correlates	690:749	Defining ECM composition involving insoluble polymeric assemblies poses unique challenges to analysis and, thus, to comparing strains with quantitative ECM molecular correlates.
32894594	5	13	theme	cellulose	896:904	arg1	ratio					906:910	the curli-to-pEtN cellulose ratio	878:910	the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110	878:975	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	5	14	theme	laboratory	947:956	arg1	AR3110					970:975	AR3110	970:975	AR3110	970:975	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	5	14	theme	laboratory	947:956	arg1	strain					962:967	the E. coli laboratory K12 strain	935:967	the E. coli laboratory K12 strain	935:967	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	9	15	from	phenotypes	1619:1628	arg1	comparison					1655:1664	the direct comparison	1644:1664	the direct comparison of these strains	1644:1681	We describe the influence of curli, cellulose, and the pEtN modification on biofilm phenotypes with power in the direct comparison of these strains.
32894594	7	16	from	plasticity	1254:1263	arg1	principles					1284:1293	matrix assembly principles	1268:1293	matrix assembly principles	1268:1293	The ECM isolated from AR3110 contains approximately twice the amount of pEtN cellulose relative to curli content as UTI89, revealing plasticity in matrix assembly principles among strains.
32894594	5	17	theme	strain	962:967	arg1	ECM					928:930	the isolated ECM	915:930	the isolated ECM of the E. coli laboratory K12 strain, AR3110	915:975	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	8	18	theme	liquid-air	1472:1481	arg1	interface					1483:1491	the liquid-air interface	1468:1491	the liquid-air interface	1468:1491	The two parent strains and a panel of relevant gene mutants were investigated in three biofilm models, examining: (a) macrocolonies on agar, (b) pellicles at the liquid-air interface, and (c) biomass accumulation on plastic.
32894594	8	19	theme	relevant	1348:1355	arg1	mutants					1362:1368	relevant gene mutants	1348:1368	relevant gene mutants	1348:1368	The two parent strains and a panel of relevant gene mutants were investigated in three biofilm models, examining: (a) macrocolonies on agar, (b) pellicles at the liquid-air interface, and (c) biomass accumulation on plastic.
32894594	7	20	theme	matrix	1268:1273	arg1	principles					1284:1293	matrix assembly principles	1268:1293	matrix assembly principles	1268:1293	The ECM isolated from AR3110 contains approximately twice the amount of pEtN cellulose relative to curli content as UTI89, revealing plasticity in matrix assembly principles among strains.
32894594	5	21	from	ratio	906:910	arg1	ECM					928:930	the isolated ECM	915:930	the isolated ECM of the E. coli laboratory K12 strain, AR3110	915:975	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	1	22	theme	bacteria	157:164	arg1	communities					142:152	communities	142:152	communities of bacteria entangled in a self-produced extracellular matrix (ECM)	142:220	Bacterial biofilms are communities of bacteria entangled in a self-produced extracellular matrix (ECM).
32894594	1	22	theme	bacteria	157:164	arg1	biofilms					129:136	Bacterial biofilms	119:136	Bacterial biofilms	119:136	Bacterial biofilms are communities of bacteria entangled in a self-produced extracellular matrix (ECM).
32894594	6	23	theme	biofilm	1047:1053	arg1	phenotypes					1055:1064	comprehensive biofilm phenotypes	1033:1064	comprehensive biofilm phenotypes	1033:1064	We compare and contrast the compositional analysis and comprehensive biofilm phenotypes for AR3110 and a well-studied clinical isolate, UTI89.
32894594	8	24	theme	mutants	1362:1368	arg1	strains					1325:1331	The two parent strains	1310:1331	The two parent strains	1310:1331	The two parent strains and a panel of relevant gene mutants were investigated in three biofilm models, examining: (a) macrocolonies on agar, (b) pellicles at the liquid-air interface, and (c) biomass accumulation on plastic.
32894594	8	24	theme	mutants	1362:1368	arg1	panel					1339:1343	a panel	1337:1343	a panel of relevant gene mutants	1337:1368	The two parent strains and a panel of relevant gene mutants were investigated in three biofilm models, examining: (a) macrocolonies on agar, (b) pellicles at the liquid-air interface, and (c) biomass accumulation on plastic.
32894594	5	25	theme	solid-state	814:824	arg1	resonance					843:851	a sum-of-the-parts 13 C solid-state nuclear magnetic resonance	790:851	a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis	790:866	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	5	25	theme	solid-state	814:824	arg1	NMR					854:856	NMR	854:856	NMR	854:856	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	5	26	theme	E.	939:940	arg1	AR3110					970:975	AR3110	970:975	AR3110	970:975	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	5	26	theme	E.	939:940	arg1	strain					962:967	the E. coli laboratory K12 strain	935:967	the E. coli laboratory K12 strain	935:967	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	0	27	from	Variation	0:8	arg1	ratio					17:21	the ratio	13:21	the ratio of curli and phosphoethanolamine	13:54	Variation in the ratio of curli and phosphoethanolamine cellulose associated with biofilm architecture and properties.
32894594	5	28	from	analysis	859:866	arg1	results					777:783	results	777:783	results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis	777:866	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	6	29	theme	compositional	1006:1018	arg1	analysis					1020:1027	the compositional analysis	1002:1027	the compositional analysis	1002:1027	We compare and contrast the compositional analysis and comprehensive biofilm phenotypes for AR3110 and a well-studied clinical isolate, UTI89.
32894594	3	30	theme	amyloid-polysaccharide	452:473	arg1	composites					475:484	these amyloid-polysaccharide composites	446:484	these amyloid-polysaccharide composites	446:484	Intense curiosity surrounds how bacteria harness these amyloid-polysaccharide composites to build biofilms, and how these biopolymers function to benefit bacterial communities.
32894594	0	31	theme	curli	26:30	arg1	ratio					17:21	the ratio	13:21	the ratio of curli and phosphoethanolamine	13:54	Variation in the ratio of curli and phosphoethanolamine cellulose associated with biofilm architecture and properties.
32894594	7	32	theme	curli	1220:1224	arg1	content					1226:1232	curli content	1220:1232	curli content as UTI89	1220:1241	The ECM isolated from AR3110 contains approximately twice the amount of pEtN cellulose relative to curli content as UTI89, revealing plasticity in matrix assembly principles among strains.
32894594	7	33	theme	relative	1208:1215	arg1	amount					1183:1188	the amount	1179:1188	the amount of pEtN cellulose relative to curli content as UTI89	1179:1241	The ECM isolated from AR3110 contains approximately twice the amount of pEtN cellulose relative to curli content as UTI89, revealing plasticity in matrix assembly principles among strains.
32894594	7	33	theme	relative	1208:1215	arg1	cellulose					1198:1206	pEtN cellulose	1193:1206	pEtN cellulose	1193:1206	The ECM isolated from AR3110 contains approximately twice the amount of pEtN cellulose relative to curli content as UTI89, revealing plasticity in matrix assembly principles among strains.
32894594	9	34	theme	curli	1564:1568	arg1	influence					1551:1559	the influence	1547:1559	the influence of curli, cellulose, and the pEtN modification on biofilm phenotypes with power in the direct comparison of these strains	1547:1681	We describe the influence of curli, cellulose, and the pEtN modification on biofilm phenotypes with power in the direct comparison of these strains.
32894594	6	35	theme	well-studied	1083:1094	arg1	UTI89					1114:1118	UTI89	1114:1118	UTI89	1114:1118	We compare and contrast the compositional analysis and comprehensive biofilm phenotypes for AR3110 and a well-studied clinical isolate, UTI89.
32894594	6	35	theme	well-studied	1083:1094	arg1	isolate					1105:1111	a well-studied clinical isolate	1081:1111	a well-studied clinical isolate	1081:1111	We compare and contrast the compositional analysis and comprehensive biofilm phenotypes for AR3110 and a well-studied clinical isolate, UTI89.
32894594	4	36	theme	insoluble	609:617	arg1	assemblies					629:638	insoluble polymeric assemblies	609:638	insoluble polymeric assemblies	609:638	Defining ECM composition involving insoluble polymeric assemblies poses unique challenges to analysis and, thus, to comparing strains with quantitative ECM molecular correlates.
32894594	5	37	theme	magnetic	834:841	arg1	resonance					843:851	a sum-of-the-parts 13 C solid-state nuclear magnetic resonance	790:851	a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis	790:866	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	5	37	theme	magnetic	834:841	arg1	NMR					854:856	NMR	854:856	NMR	854:856	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	7	38	theme	pEtN	1193:1196	arg1	cellulose					1198:1206	pEtN cellulose	1193:1206	pEtN cellulose	1193:1206	The ECM isolated from AR3110 contains approximately twice the amount of pEtN cellulose relative to curli content as UTI89, revealing plasticity in matrix assembly principles among strains.
32894594	9	39	theme	cellulose	1571:1579	arg1	influence					1551:1559	the influence	1547:1559	the influence of curli, cellulose, and the pEtN modification on biofilm phenotypes with power in the direct comparison of these strains	1547:1681	We describe the influence of curli, cellulose, and the pEtN modification on biofilm phenotypes with power in the direct comparison of these strains.
32894594	4	40	with	strains	700:706	arg1	correlates					740:749	quantitative ECM molecular correlates	713:749	quantitative ECM molecular correlates	713:749	Defining ECM composition involving insoluble polymeric assemblies poses unique challenges to analysis and, thus, to comparing strains with quantitative ECM molecular correlates.
32894594	2	41	theme	cellulose	343:351	arg1	assembly					251:258	the assembly	247:258	the assembly of two insoluble biopolymers, curli amyloid fibers, and phosphoethanolamine (pEtN) cellulose	247:351	Escherichia coli direct the assembly of two insoluble biopolymers, curli amyloid fibers, and phosphoethanolamine (pEtN) cellulose, to build remarkable biofilm architectures.
32894594	2	42	theme	pEtN	337:340	arg1	cellulose					343:351	phosphoethanolamine (pEtN) cellulose	316:351	phosphoethanolamine (pEtN) cellulose	316:351	Escherichia coli direct the assembly of two insoluble biopolymers, curli amyloid fibers, and phosphoethanolamine (pEtN) cellulose, to build remarkable biofilm architectures.
32894594	8	43	dep	macrocolonies	1428:1440	arg1	a					1425:1425	a	1425:1425	a	1425:1425	The two parent strains and a panel of relevant gene mutants were investigated in three biofilm models, examining: (a) macrocolonies on agar, (b) pellicles at the liquid-air interface, and (c) biomass accumulation on plastic.
32894594	8	43	dep	macrocolonies	1428:1440	arg1	pellicles					1455:1463	pellicles	1455:1463	pellicles at the liquid-air interface, and (c) biomass accumulation on plastic	1455:1532	The two parent strains and a panel of relevant gene mutants were investigated in three biofilm models, examining: (a) macrocolonies on agar, (b) pellicles at the liquid-air interface, and (c) biomass accumulation on plastic.
32894594	8	43	dep	macrocolonies	1428:1440	arg1	b					1452:1452	b	1452:1452	b	1452:1452	The two parent strains and a panel of relevant gene mutants were investigated in three biofilm models, examining: (a) macrocolonies on agar, (b) pellicles at the liquid-air interface, and (c) biomass accumulation on plastic.
32894594	9	44	theme	pEtN	1590:1593	arg1	modification					1595:1606	the pEtN modification	1586:1606	the pEtN modification	1586:1606	We describe the influence of curli, cellulose, and the pEtN modification on biofilm phenotypes with power in the direct comparison of these strains.
32894594	5	45	attach	present	769:775	arg2	we					766:767	we	766:767	we	766:767	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	5	45	attach	present	769:775	arg1	work					760:763	this work	755:763	this work	755:763	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	2	46	theme	remarkable	363:372	arg1	architectures					382:394	remarkable biofilm architectures	363:394	remarkable biofilm architectures	363:394	Escherichia coli direct the assembly of two insoluble biopolymers, curli amyloid fibers, and phosphoethanolamine (pEtN) cellulose, to build remarkable biofilm architectures.
32894594	5	47	theme	resonance	843:851	arg1	analysis					859:866	a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis	790:866	a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis	790:866	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	5	48	theme	C	812:812	arg1	resonance					843:851	a sum-of-the-parts 13 C solid-state nuclear magnetic resonance	790:851	a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis	790:866	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	5	48	theme	C	812:812	arg1	NMR					854:856	NMR	854:856	NMR	854:856	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	2	49	theme	amyloid	296:302	arg1	fibers					304:309	curli amyloid fibers	290:309	curli amyloid fibers	290:309	Escherichia coli direct the assembly of two insoluble biopolymers, curli amyloid fibers, and phosphoethanolamine (pEtN) cellulose, to build remarkable biofilm architectures.
32894594	4	50	theme	unique	646:651	arg1	challenges					653:662	unique challenges	646:662	unique challenges	646:662	Defining ECM composition involving insoluble polymeric assemblies poses unique challenges to analysis and, thus, to comparing strains with quantitative ECM molecular correlates.
32894594	1	51	theme	extracellular	195:207	arg1	ECM					217:219	ECM	217:219	ECM	217:219	Bacterial biofilms are communities of bacteria entangled in a self-produced extracellular matrix (ECM).
32894594	1	51	theme	extracellular	195:207	arg1	matrix					209:214	a self-produced extracellular matrix	179:214	a self-produced extracellular matrix (ECM)	179:220	Bacterial biofilms are communities of bacteria entangled in a self-produced extracellular matrix (ECM).
32894594	2	52	theme	phosphoethanolamine	316:334	arg1	cellulose					343:351	phosphoethanolamine (pEtN) cellulose	316:351	phosphoethanolamine (pEtN) cellulose	316:351	Escherichia coli direct the assembly of two insoluble biopolymers, curli amyloid fibers, and phosphoethanolamine (pEtN) cellulose, to build remarkable biofilm architectures.
32894594	8	53	dep	models	1405:1410	arg1	macrocolonies					1428:1440	macrocolonies	1428:1440	macrocolonies on agar	1428:1448	The two parent strains and a panel of relevant gene mutants were investigated in three biofilm models, examining: (a) macrocolonies on agar, (b) pellicles at the liquid-air interface, and (c) biomass accumulation on plastic.
32894594	4	54	theme	quantitative	713:724	arg1	correlates					740:749	quantitative ECM molecular correlates	713:749	quantitative ECM molecular correlates	713:749	Defining ECM composition involving insoluble polymeric assemblies poses unique challenges to analysis and, thus, to comparing strains with quantitative ECM molecular correlates.
32894594	5	55	theme	curli-to-pEtN	882:894	arg1	ratio					906:910	the curli-to-pEtN cellulose ratio	878:910	the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110	878:975	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	11	56	theme	combined	1808:1815	arg1	influence					1817:1825	Their individual and combined influence	1787:1825	Their individual and combined influence	1787:1825	Their individual and combined influence depends on both the biofilm modality (agar, pellicle, or plastic-associated) and the strain itself.
32894594	1	57	theme	Bacterial	119:127	arg1	communities					142:152	communities	142:152	communities of bacteria entangled in a self-produced extracellular matrix (ECM)	142:220	Bacterial biofilms are communities of bacteria entangled in a self-produced extracellular matrix (ECM).
32894594	1	57	theme	Bacterial	119:127	arg1	biofilms					129:136	Bacterial biofilms	119:136	Bacterial biofilms	119:136	Bacterial biofilms are communities of bacteria entangled in a self-produced extracellular matrix (ECM).
32894594	11	58	theme	individual	1793:1802	arg1	influence					1817:1825	Their individual and combined influence	1787:1825	Their individual and combined influence	1787:1825	Their individual and combined influence depends on both the biofilm modality (agar, pellicle, or plastic-associated) and the strain itself.
32894594	2	59	theme	insoluble	267:275	arg1	biopolymers					277:287	two insoluble biopolymers	263:287	two insoluble biopolymers	263:287	Escherichia coli direct the assembly of two insoluble biopolymers, curli amyloid fibers, and phosphoethanolamine (pEtN) cellulose, to build remarkable biofilm architectures.
32894594	5	60	theme	K12	958:960	arg1	AR3110					970:975	AR3110	970:975	AR3110	970:975	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	5	60	theme	K12	958:960	arg1	strain					962:967	the E. coli laboratory K12 strain	935:967	the E. coli laboratory K12 strain	935:967	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	5	61	theme	sum-of-the-parts	792:807	arg1	resonance					843:851	a sum-of-the-parts 13 C solid-state nuclear magnetic resonance	790:851	a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis	790:866	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	5	61	theme	sum-of-the-parts	792:807	arg1	NMR					854:856	NMR	854:856	NMR	854:856	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	9	62	from	influence	1551:1559	arg1	phenotypes					1619:1628	biofilm phenotypes	1611:1628	biofilm phenotypes with power in the direct comparison of these strains	1611:1681	We describe the influence of curli, cellulose, and the pEtN modification on biofilm phenotypes with power in the direct comparison of these strains.
32894594	4	63	theme	molecular	730:738	arg1	correlates					740:749	quantitative ECM molecular correlates	713:749	quantitative ECM molecular correlates	713:749	Defining ECM composition involving insoluble polymeric assemblies poses unique challenges to analysis and, thus, to comparing strains with quantitative ECM molecular correlates.
32894594	7	64	contain	contains	1150:1157	arg1	ECM					1125:1127	The ECM	1121:1127	The ECM isolated from AR3110	1121:1148	The ECM isolated from AR3110 contains approximately twice the amount of pEtN cellulose relative to curli content as UTI89, revealing plasticity in matrix assembly principles among strains.
32894594	7	64	contain	contains	1150:1157	arg2	amount					1183:1188	the amount	1179:1188	the amount of pEtN cellulose relative to curli content as UTI89	1179:1241	The ECM isolated from AR3110 contains approximately twice the amount of pEtN cellulose relative to curli content as UTI89, revealing plasticity in matrix assembly principles among strains.
32894594	7	64	contain	contains	1150:1157	arg2	cellulose					1198:1206	pEtN cellulose	1193:1206	pEtN cellulose	1193:1206	The ECM isolated from AR3110 contains approximately twice the amount of pEtN cellulose relative to curli content as UTI89, revealing plasticity in matrix assembly principles among strains.
32894594	0	65	theme	biofilm	82:88	arg1	architecture					90:101	biofilm architecture	82:101	biofilm architecture	82:101	Variation in the ratio of curli and phosphoethanolamine cellulose associated with biofilm architecture and properties.
32894594	11	66	theme	biofilm	1847:1853	arg1	modality					1855:1862	the biofilm modality	1843:1862	the biofilm modality (agar, pellicle, or plastic-associated)	1843:1902	Their individual and combined influence depends on both the biofilm modality (agar, pellicle, or plastic-associated) and the strain itself.
32894594	6	67	theme	comprehensive	1033:1045	arg1	phenotypes					1055:1064	comprehensive biofilm phenotypes	1033:1064	comprehensive biofilm phenotypes	1033:1064	We compare and contrast the compositional analysis and comprehensive biofilm phenotypes for AR3110 and a well-studied clinical isolate, UTI89.
32894594	8	68	theme	gene	1357:1360	arg1	mutants					1362:1368	relevant gene mutants	1348:1368	relevant gene mutants	1348:1368	The two parent strains and a panel of relevant gene mutants were investigated in three biofilm models, examining: (a) macrocolonies on agar, (b) pellicles at the liquid-air interface, and (c) biomass accumulation on plastic.
32894594	8	69	theme	biomass	1502:1508	arg1	accumulation					1510:1521	(c) biomass accumulation	1498:1521	(c) biomass accumulation	1498:1521	The two parent strains and a panel of relevant gene mutants were investigated in three biofilm models, examining: (a) macrocolonies on agar, (b) pellicles at the liquid-air interface, and (c) biomass accumulation on plastic.
32894594	3	70	dep	surrounds	415:423	arg1	harness					438:444	harness	438:444	harness these amyloid-polysaccharide composites to build biofilms	438:502	Intense curiosity surrounds how bacteria harness these amyloid-polysaccharide composites to build biofilms, and how these biopolymers function to benefit bacterial communities.
32894594	3	70	dep	surrounds	415:423	arg1	function					531:538	function	531:538	function to benefit bacterial communities	531:571	Intense curiosity surrounds how bacteria harness these amyloid-polysaccharide composites to build biofilms, and how these biopolymers function to benefit bacterial communities.
32894594	3	71	theme	Intense	397:403	arg1	curiosity					405:413	Intense curiosity	397:413	Intense curiosity	397:413	Intense curiosity surrounds how bacteria harness these amyloid-polysaccharide composites to build biofilms, and how these biopolymers function to benefit bacterial communities.
32894594	5	72	dep	E.	939:940	arg1	coli					942:945	coli	942:945	coli	942:945	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	2	73	theme	biofilm	374:380	arg1	architectures					382:394	remarkable biofilm architectures	363:394	remarkable biofilm architectures	363:394	Escherichia coli direct the assembly of two insoluble biopolymers, curli amyloid fibers, and phosphoethanolamine (pEtN) cellulose, to build remarkable biofilm architectures.
32894594	7	74	theme	cellulose	1198:1206	arg1	amount					1183:1188	the amount	1179:1188	the amount of pEtN cellulose relative to curli content as UTI89	1179:1241	The ECM isolated from AR3110 contains approximately twice the amount of pEtN cellulose relative to curli content as UTI89, revealing plasticity in matrix assembly principles among strains.
32894594	7	74	theme	cellulose	1198:1206	arg1	cellulose					1198:1206	pEtN cellulose	1193:1206	pEtN cellulose	1193:1206	The ECM isolated from AR3110 contains approximately twice the amount of pEtN cellulose relative to curli content as UTI89, revealing plasticity in matrix assembly principles among strains.
32894594	11	75	dep	modality	1855:1862	arg1	pellicle					1871:1878	pellicle	1871:1878	pellicle	1871:1878	Their individual and combined influence depends on both the biofilm modality (agar, pellicle, or plastic-associated) and the strain itself.
32894594	11	75	dep	modality	1855:1862	arg1	plastic-associated					1884:1901	plastic-associated	1884:1901	plastic-associated	1884:1901	Their individual and combined influence depends on both the biofilm modality (agar, pellicle, or plastic-associated) and the strain itself.
32894594	11	75	dep	modality	1855:1862	arg1	agar					1865:1868	agar	1865:1868	agar	1865:1868	Their individual and combined influence depends on both the biofilm modality (agar, pellicle, or plastic-associated) and the strain itself.
32894594	6	76	theme	clinical	1096:1103	arg1	UTI89					1114:1118	UTI89	1114:1118	UTI89	1114:1118	We compare and contrast the compositional analysis and comprehensive biofilm phenotypes for AR3110 and a well-studied clinical isolate, UTI89.
32894594	6	76	theme	clinical	1096:1103	arg1	isolate					1105:1111	a well-studied clinical isolate	1081:1111	a well-studied clinical isolate	1081:1111	We compare and contrast the compositional analysis and comprehensive biofilm phenotypes for AR3110 and a well-studied clinical isolate, UTI89.
32894594	4	77	theme	polymeric	619:627	arg1	assemblies					629:638	insoluble polymeric assemblies	609:638	insoluble polymeric assemblies	609:638	Defining ECM composition involving insoluble polymeric assemblies poses unique challenges to analysis and, thus, to comparing strains with quantitative ECM molecular correlates.
32894594	5	78	theme	nuclear	826:832	arg1	resonance					843:851	a sum-of-the-parts 13 C solid-state nuclear magnetic resonance	790:851	a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis	790:866	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	5	78	theme	nuclear	826:832	arg1	NMR					854:856	NMR	854:856	NMR	854:856	In this work, we present results from a sum-of-the-parts 13 C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of the E. coli laboratory K12 strain, AR3110.
32894594	8	79	theme	parent	1318:1323	arg1	strains					1325:1331	The two parent strains	1310:1331	The two parent strains	1310:1331	The two parent strains and a panel of relevant gene mutants were investigated in three biofilm models, examining: (a) macrocolonies on agar, (b) pellicles at the liquid-air interface, and (c) biomass accumulation on plastic.
32894594	0	80	theme	phosphoethanolamine	36:54	arg1	ratio					17:21	the ratio	13:21	the ratio of curli and phosphoethanolamine	13:54	Variation in the ratio of curli and phosphoethanolamine cellulose associated with biofilm architecture and properties.
32894594	4	81	theme	ECM	583:585	arg1	composition					587:597	ECM composition	583:597	ECM composition involving insoluble polymeric assemblies	583:638	Defining ECM composition involving insoluble polymeric assemblies poses unique challenges to analysis and, thus, to comparing strains with quantitative ECM molecular correlates.
32894594	7	82	attach	isolated	1129:1136	arg1	AR3110					1143:1148	AR3110	1143:1148	AR3110	1143:1148	The ECM isolated from AR3110 contains approximately twice the amount of pEtN cellulose relative to curli content as UTI89, revealing plasticity in matrix assembly principles among strains.
32894594	7	82	attach	isolated	1129:1136	arg2	ECM					1125:1127	The ECM	1121:1127	The ECM isolated from AR3110	1121:1148	The ECM isolated from AR3110 contains approximately twice the amount of pEtN cellulose relative to curli content as UTI89, revealing plasticity in matrix assembly principles among strains.
32894594	8	83	dep	accumulation	1510:1521	arg1	c					1499:1499	c	1499:1499	c	1499:1499	The two parent strains and a panel of relevant gene mutants were investigated in three biofilm models, examining: (a) macrocolonies on agar, (b) pellicles at the liquid-air interface, and (c) biomass accumulation on plastic.
32894594	9	84	theme	modification	1595:1606	arg1	influence					1551:1559	the influence	1547:1559	the influence of curli, cellulose, and the pEtN modification on biofilm phenotypes with power in the direct comparison of these strains	1547:1681	We describe the influence of curli, cellulose, and the pEtN modification on biofilm phenotypes with power in the direct comparison of these strains.
32894594	3	85	theme	bacterial	551:559	arg1	communities					561:571	bacterial communities	551:571	bacterial communities	551:571	Intense curiosity surrounds how bacteria harness these amyloid-polysaccharide composites to build biofilms, and how these biopolymers function to benefit bacterial communities.
33483029	4	0	theme	crosslinking	749:760	arg1	density					762:768	crosslinking density	749:768	crosslinking density	749:768	A significant increase in crosslinking density and glass transition temperature of the composite films were observed as the CA contents increased, which was attributed to the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA.
33483029	2	1	theme	size	401:404	arg1	distribution					406:417	particle size distribution	392:417	particle size distribution	392:417	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	0	2	theme	carboxymethyl	99:111	arg1	chitosan					113:120	carboxymethyl chitosan	99:120	carboxymethyl chitosan	99:120	Semi-interpenetrating polymer networks prepared from castor oil-based waterborne polyurethanes and carboxymethyl chitosan.
33483029	4	3	theme	films	925:929	arg1	segment					914:920	the increasing hard segment	894:920	the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA	894:998	A significant increase in crosslinking density and glass transition temperature of the composite films were observed as the CA contents increased, which was attributed to the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA.
33483029	2	4	theme	particle	392:399	arg1	distribution					406:417	particle size distribution	392:417	particle size distribution	392:417	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	4	5	theme	hydrogen	942:949	arg1	interaction					959:969	strong hydrogen bonding interaction	935:969	strong hydrogen bonding interaction between polyurethanes and CA	935:998	A significant increase in crosslinking density and glass transition temperature of the composite films were observed as the CA contents increased, which was attributed to the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA.
33483029	4	6	theme	films	820:824	arg1	temperature					791:801	glass transition temperature	774:801	glass transition temperature of the composite films	774:824	A significant increase in crosslinking density and glass transition temperature of the composite films were observed as the CA contents increased, which was attributed to the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA.
33483029	4	6	theme	films	820:824	arg1	increase					737:744	A significant increase	723:744	A significant increase in crosslinking density	723:768	A significant increase in crosslinking density and glass transition temperature of the composite films were observed as the CA contents increased, which was attributed to the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA.
33483029	1	7	theme	secondary	314:322	arg1	phase					332:336	the secondary polymer phase	310:336	the secondary polymer phase	310:336	A series of vegetable oil-based waterborne polyurethane composites were prepared through construction of novel semi-interpenetrating polymers network using carboxymethyl chitosan (CA) as the secondary polymer phase.
33483029	1	7	theme	secondary	314:322	arg1	chitosan					293:300	carboxymethyl chitosan	279:300	carboxymethyl chitosan (CA)	279:305	A series of vegetable oil-based waterborne polyurethane composites were prepared through construction of novel semi-interpenetrating polymers network using carboxymethyl chitosan (CA) as the secondary polymer phase.
33483029	4	8	theme	strong	935:940	arg1	interaction					959:969	strong hydrogen bonding interaction	935:969	strong hydrogen bonding interaction between polyurethanes and CA	935:998	A significant increase in crosslinking density and glass transition temperature of the composite films were observed as the CA contents increased, which was attributed to the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA.
33483029	4	9	from	increase	737:744	arg1	density					762:768	crosslinking density	749:768	crosslinking density	749:768	A significant increase in crosslinking density and glass transition temperature of the composite films were observed as the CA contents increased, which was attributed to the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA.
33483029	2	10	theme	storage	369:375	arg1	stability					377:385	storage stability	369:385	storage stability	369:385	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	5	11	from	application	1152:1162	arg1	field					1171:1175	the field	1167:1175	the field of coatings, adhesives, ink and so on	1167:1213	This work provided a simple method to tailor the performance of environmentally friendly vegetable oil-based waterborne polyurethane, which could find application in the field of coatings, adhesives, ink and so on.
33483029	4	12	theme	transition	780:789	arg1	temperature					791:801	glass transition temperature	774:801	glass transition temperature of the composite films	774:824	A significant increase in crosslinking density and glass transition temperature of the composite films were observed as the CA contents increased, which was attributed to the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA.
33483029	4	13	theme	hard	909:912	arg1	segment					914:920	the increasing hard segment	894:920	the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA	894:998	A significant increase in crosslinking density and glass transition temperature of the composite films were observed as the CA contents increased, which was attributed to the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA.
33483029	4	14	theme	CA	847:848	arg1	contents					850:857	the CA contents	843:857	the CA contents	843:857	A significant increase in crosslinking density and glass transition temperature of the composite films were observed as the CA contents increased, which was attributed to the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA.
33483029	2	15	theme	composite	426:434	arg1	dispersions					436:446	the composite dispersions	422:446	the composite dispersions	422:446	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	1	16	theme	polymer	324:330	arg1	phase					332:336	the secondary polymer phase	310:336	the secondary polymer phase	310:336	A series of vegetable oil-based waterborne polyurethane composites were prepared through construction of novel semi-interpenetrating polymers network using carboxymethyl chitosan (CA) as the secondary polymer phase.
33483029	1	16	theme	polymer	324:330	arg1	chitosan					293:300	carboxymethyl chitosan	279:300	carboxymethyl chitosan (CA)	279:305	A series of vegetable oil-based waterborne polyurethane composites were prepared through construction of novel semi-interpenetrating polymers network using carboxymethyl chitosan (CA) as the secondary polymer phase.
33483029	4	17	theme	increasing	898:907	arg1	segment					914:920	the increasing hard segment	894:920	the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA	894:998	A significant increase in crosslinking density and glass transition temperature of the composite films were observed as the CA contents increased, which was attributed to the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA.
33483029	0	18	theme	Semi-interpenetrating	0:20	arg1	networks					30:37	Semi-interpenetrating polymer networks	0:37	Semi-interpenetrating polymer networks prepared from castor	0:58	Semi-interpenetrating polymer networks prepared from castor oil-based waterborne polyurethanes and carboxymethyl chitosan.
33483029	2	19	theme	dispersions	436:446	arg1	stability					460:468	thermal stability	452:468	thermal stability	452:468	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	2	19	theme	dispersions	436:446	arg1	properties					482:491	mechanical properties	471:491	mechanical properties	471:491	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	2	19	theme	dispersions	436:446	arg1	wettability					505:515	surface wettability	497:515	surface wettability	497:515	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	2	19	theme	dispersions	436:446	arg1	distribution					406:417	particle size distribution	392:417	particle size distribution	392:417	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	2	19	theme	dispersions	436:446	arg1	stability					377:385	storage stability	369:385	storage stability	369:385	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	0	20	theme	polymer	22:28	arg1	networks					30:37	Semi-interpenetrating polymer networks	0:37	Semi-interpenetrating polymer networks prepared from castor	0:58	Semi-interpenetrating polymer networks prepared from castor oil-based waterborne polyurethanes and carboxymethyl chitosan.
33483029	4	21	theme	composite	810:818	arg1	films					820:824	the composite films	806:824	the composite films	806:824	A significant increase in crosslinking density and glass transition temperature of the composite films were observed as the CA contents increased, which was attributed to the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA.
33483029	5	22	theme	coatings	1180:1187	arg1	field					1171:1175	the field	1167:1175	the field of coatings, adhesives, ink and so on	1167:1213	This work provided a simple method to tailor the performance of environmentally friendly vegetable oil-based waterborne polyurethane, which could find application in the field of coatings, adhesives, ink and so on.
33483029	1	23	theme	novel	228:232	arg1	network					265:271	novel semi-interpenetrating polymers network	228:271	novel semi-interpenetrating polymers network using carboxymethyl chitosan (CA) as the secondary polymer phase	228:336	A series of vegetable oil-based waterborne polyurethane composites were prepared through construction of novel semi-interpenetrating polymers network using carboxymethyl chitosan (CA) as the secondary polymer phase.
33483029	2	24	theme	surface	497:503	arg1	wettability					505:515	surface wettability	497:515	surface wettability	497:515	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	5	25	dep	and	1205:1207	arg1	so					1209:1210	so	1209:1210	so	1209:1210	This work provided a simple method to tailor the performance of environmentally friendly vegetable oil-based waterborne polyurethane, which could find application in the field of coatings, adhesives, ink and so on.
33483029	2	26	theme	contents	357:364	arg1	effects					343:349	The effects	339:349	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films	339:534	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	1	27	theme	semi-interpenetrating	234:254	arg1	network					265:271	novel semi-interpenetrating polymers network	228:271	novel semi-interpenetrating polymers network using carboxymethyl chitosan (CA) as the secondary polymer phase	228:336	A series of vegetable oil-based waterborne polyurethane composites were prepared through construction of novel semi-interpenetrating polymers network using carboxymethyl chitosan (CA) as the secondary polymer phase.
33483029	5	28	theme	environmentally	1065:1079	arg1	polyurethane					1121:1132	environmentally friendly vegetable oil-based waterborne polyurethane	1065:1132	environmentally friendly vegetable oil-based waterborne polyurethane	1065:1132	This work provided a simple method to tailor the performance of environmentally friendly vegetable oil-based waterborne polyurethane, which could find application in the field of coatings, adhesives, ink and so on.
33483029	5	29	theme	polyurethane	1121:1132	arg1	performance					1050:1060	the performance	1046:1060	the performance of environmentally friendly vegetable oil-based waterborne polyurethane, which could find application in the field of coatings, adhesives, ink and so on	1046:1213	This work provided a simple method to tailor the performance of environmentally friendly vegetable oil-based waterborne polyurethane, which could find application in the field of coatings, adhesives, ink and so on.
33483029	2	30	theme	CA	354:355	arg1	contents					357:364	CA contents	354:364	CA contents	354:364	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	5	31	theme	simple	1022:1027	arg1	method					1029:1034	a simple method	1020:1034	a simple method to tailor the performance of environmentally friendly vegetable oil-based waterborne polyurethane, which could find application in the field of coatings, adhesives, ink and so on	1020:1213	This work provided a simple method to tailor the performance of environmentally friendly vegetable oil-based waterborne polyurethane, which could find application in the field of coatings, adhesives, ink and so on.
33483029	1	32	theme	polymers	256:263	arg1	network					265:271	novel semi-interpenetrating polymers network	228:271	novel semi-interpenetrating polymers network using carboxymethyl chitosan (CA) as the secondary polymer phase	228:336	A series of vegetable oil-based waterborne polyurethane composites were prepared through construction of novel semi-interpenetrating polymers network using carboxymethyl chitosan (CA) as the secondary polymer phase.
33483029	3	33	theme	storage	639:645	arg1	stability					647:655	excellent storage stability	629:655	excellent storage stability	629:655	The results showed that the composite dispersions displayed excellent storage stability and the biomass contents of resulting films were high up to 80 %.
33483029	1	34	theme	network	265:271	arg1	construction					212:223	construction	212:223	construction of novel semi-interpenetrating polymers network using carboxymethyl chitosan (CA) as the secondary polymer phase	212:336	A series of vegetable oil-based waterborne polyurethane composites were prepared through construction of novel semi-interpenetrating polymers network using carboxymethyl chitosan (CA) as the secondary polymer phase.
33483029	4	35	theme	glass	774:778	arg1	transition					780:789	glass transition	774:789	glass transition temperature of the composite films	774:824	A significant increase in crosslinking density and glass transition temperature of the composite films were observed as the CA contents increased, which was attributed to the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA.
33483029	5	36	theme	vegetable	1090:1098	arg1	polyurethane					1121:1132	environmentally friendly vegetable oil-based waterborne polyurethane	1065:1132	environmentally friendly vegetable oil-based waterborne polyurethane	1065:1132	This work provided a simple method to tailor the performance of environmentally friendly vegetable oil-based waterborne polyurethane, which could find application in the field of coatings, adhesives, ink and so on.
33483029	4	37	theme	significant	725:735	arg1	increase					737:744	A significant increase	723:744	A significant increase in crosslinking density	723:768	A significant increase in crosslinking density and glass transition temperature of the composite films were observed as the CA contents increased, which was attributed to the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA.
33483029	2	38	theme	films	530:534	arg1	stability					460:468	thermal stability	452:468	thermal stability	452:468	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	2	38	theme	films	530:534	arg1	properties					482:491	mechanical properties	471:491	mechanical properties	471:491	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	2	38	theme	films	530:534	arg1	wettability					505:515	surface wettability	497:515	surface wettability	497:515	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	2	38	theme	films	530:534	arg1	distribution					406:417	particle size distribution	392:417	particle size distribution	392:417	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	2	38	theme	films	530:534	arg1	stability					377:385	storage stability	369:385	storage stability	369:385	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	5	39	theme	on	1212:1213	arg1	field					1171:1175	the field	1167:1175	the field of coatings, adhesives, ink and so on	1167:1213	This work provided a simple method to tailor the performance of environmentally friendly vegetable oil-based waterborne polyurethane, which could find application in the field of coatings, adhesives, ink and so on.
33483029	5	40	theme	oil-based	1100:1108	arg1	polyurethane					1121:1132	environmentally friendly vegetable oil-based waterborne polyurethane	1065:1132	environmentally friendly vegetable oil-based waterborne polyurethane	1065:1132	This work provided a simple method to tailor the performance of environmentally friendly vegetable oil-based waterborne polyurethane, which could find application in the field of coatings, adhesives, ink and so on.
33483029	1	41	theme	carboxymethyl	279:291	arg1	CA					303:304	CA	303:304	CA	303:304	A series of vegetable oil-based waterborne polyurethane composites were prepared through construction of novel semi-interpenetrating polymers network using carboxymethyl chitosan (CA) as the secondary polymer phase.
33483029	1	41	theme	carboxymethyl	279:291	arg1	chitosan					293:300	carboxymethyl chitosan	279:300	carboxymethyl chitosan (CA)	279:305	A series of vegetable oil-based waterborne polyurethane composites were prepared through construction of novel semi-interpenetrating polymers network using carboxymethyl chitosan (CA) as the secondary polymer phase.
33483029	1	41	theme	carboxymethyl	279:291	arg1	phase					332:336	the secondary polymer phase	310:336	the secondary polymer phase	310:336	A series of vegetable oil-based waterborne polyurethane composites were prepared through construction of novel semi-interpenetrating polymers network using carboxymethyl chitosan (CA) as the secondary polymer phase.
33483029	0	42	theme	waterborne	70:79	arg1	polyurethanes					81:93	waterborne polyurethanes	70:93	waterborne polyurethanes	70:93	Semi-interpenetrating polymer networks prepared from castor oil-based waterborne polyurethanes and carboxymethyl chitosan.
33483029	4	43	theme	interaction	959:969	arg1	segment					914:920	the increasing hard segment	894:920	the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA	894:998	A significant increase in crosslinking density and glass transition temperature of the composite films were observed as the CA contents increased, which was attributed to the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA.
33483029	5	44	theme	waterborne	1110:1119	arg1	polyurethane					1121:1132	environmentally friendly vegetable oil-based waterborne polyurethane	1065:1132	environmentally friendly vegetable oil-based waterborne polyurethane	1065:1132	This work provided a simple method to tailor the performance of environmentally friendly vegetable oil-based waterborne polyurethane, which could find application in the field of coatings, adhesives, ink and so on.
33483029	1	45	theme	vegetable	135:143	arg1	composites					179:188	vegetable oil-based waterborne polyurethane composites	135:188	vegetable oil-based waterborne polyurethane composites	135:188	A series of vegetable oil-based waterborne polyurethane composites were prepared through construction of novel semi-interpenetrating polymers network using carboxymethyl chitosan (CA) as the secondary polymer phase.
33483029	4	46	theme	bonding	951:957	arg1	interaction					959:969	strong hydrogen bonding interaction	935:969	strong hydrogen bonding interaction between polyurethanes and CA	935:998	A significant increase in crosslinking density and glass transition temperature of the composite films were observed as the CA contents increased, which was attributed to the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA.
33483029	3	47	theme	biomass	665:671	arg1	contents					673:680	the biomass contents	661:680	the biomass contents of resulting films	661:699	The results showed that the composite dispersions displayed excellent storage stability and the biomass contents of resulting films were high up to 80 %.
33483029	3	47	theme	biomass	665:671	arg1	%					720:720	high up to 80 %	706:720	high up to 80 %	706:720	The results showed that the composite dispersions displayed excellent storage stability and the biomass contents of resulting films were high up to 80 %.
33483029	1	48	theme	oil-based	145:153	arg1	composites					179:188	vegetable oil-based waterborne polyurethane composites	135:188	vegetable oil-based waterborne polyurethane composites	135:188	A series of vegetable oil-based waterborne polyurethane composites were prepared through construction of novel semi-interpenetrating polymers network using carboxymethyl chitosan (CA) as the secondary polymer phase.
33483029	5	49	theme	friendly	1081:1088	arg1	polyurethane					1121:1132	environmentally friendly vegetable oil-based waterborne polyurethane	1065:1132	environmentally friendly vegetable oil-based waterborne polyurethane	1065:1132	This work provided a simple method to tailor the performance of environmentally friendly vegetable oil-based waterborne polyurethane, which could find application in the field of coatings, adhesives, ink and so on.
33483029	1	50	theme	waterborne	155:164	arg1	composites					179:188	vegetable oil-based waterborne polyurethane composites	135:188	vegetable oil-based waterborne polyurethane composites	135:188	A series of vegetable oil-based waterborne polyurethane composites were prepared through construction of novel semi-interpenetrating polymers network using carboxymethyl chitosan (CA) as the secondary polymer phase.
33483029	3	51	theme	excellent	629:637	arg1	stability					647:655	excellent storage stability	629:655	excellent storage stability	629:655	The results showed that the composite dispersions displayed excellent storage stability and the biomass contents of resulting films were high up to 80 %.
33483029	3	52	theme	composite	597:605	arg1	dispersions					607:617	the composite dispersions	593:617	the composite dispersions	593:617	The results showed that the composite dispersions displayed excellent storage stability and the biomass contents of resulting films were high up to 80 %.
33483029	1	53	theme	polyurethane	166:177	arg1	composites					179:188	vegetable oil-based waterborne polyurethane composites	135:188	vegetable oil-based waterborne polyurethane composites	135:188	A series of vegetable oil-based waterborne polyurethane composites were prepared through construction of novel semi-interpenetrating polymers network using carboxymethyl chitosan (CA) as the secondary polymer phase.
33483029	2	54	from	effects	343:349	arg1	stability					460:468	thermal stability	452:468	thermal stability	452:468	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	2	54	from	effects	343:349	arg1	distribution					406:417	particle size distribution	392:417	particle size distribution	392:417	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	2	54	from	effects	343:349	arg1	stability					377:385	storage stability	369:385	storage stability	369:385	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	2	55	theme	thermal	452:458	arg1	stability					460:468	thermal stability	452:468	thermal stability	452:468	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	3	56	theme	resulting	685:693	arg1	films					695:699	resulting films	685:699	resulting films	685:699	The results showed that the composite dispersions displayed excellent storage stability and the biomass contents of resulting films were high up to 80 %.
33483029	2	57	theme	mechanical	471:480	arg1	properties					482:491	mechanical properties	471:491	mechanical properties	471:491	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	1	58	theme	composites	179:188	arg1	series					125:130	A series	123:130	A series of vegetable oil-based waterborne polyurethane composites	123:188	A series of vegetable oil-based waterborne polyurethane composites were prepared through construction of novel semi-interpenetrating polymers network using carboxymethyl chitosan (CA) as the secondary polymer phase.
33483029	4	59	from	temperature	791:801	arg1	density					762:768	crosslinking density	749:768	crosslinking density	749:768	A significant increase in crosslinking density and glass transition temperature of the composite films were observed as the CA contents increased, which was attributed to the increasing hard segment of films and strong hydrogen bonding interaction between polyurethanes and CA.
33483029	3	60	dep	80	717:718	arg1	up					711:712	up	711:712	up	711:712	The results showed that the composite dispersions displayed excellent storage stability and the biomass contents of resulting films were high up to 80 %.
33483029	3	60	dep	80	717:718	arg1	to					714:715	to	714:715	to	714:715	The results showed that the composite dispersions displayed excellent storage stability and the biomass contents of resulting films were high up to 80 %.
33483029	2	61	theme	composite	520:528	arg1	films					530:534	composite films	520:534	composite films	520:534	The effects of CA contents on storage stability, and particle size distribution of the composite dispersions and thermal stability, mechanical properties and surface wettability of composite films were investigated and discussed.
33483029	3	62	theme	films	695:699	arg1	contents					673:680	the biomass contents	661:680	the biomass contents of resulting films	661:699	The results showed that the composite dispersions displayed excellent storage stability and the biomass contents of resulting films were high up to 80 %.
33483029	3	62	theme	films	695:699	arg1	%					720:720	high up to 80 %	706:720	high up to 80 %	706:720	The results showed that the composite dispersions displayed excellent storage stability and the biomass contents of resulting films were high up to 80 %.
34167634	11	0	theme	therapeutic	1728:1738	arg1	factors					1750:1756	the suitable next generation therapeutic potential factors	1699:1756	the suitable next generation therapeutic potential factors for prevention and treatment of antibiotic-resistant pneumonia	1699:1819	In conclusion, we expect biomimicking systems combined with the effective antibacterial agent could be the suitable next generation therapeutic potential factors for prevention and treatment of antibiotic-resistant pneumonia.
34167634	5	1	theme	Fe₂O₃	727:731	arg1	NPs					733:735	Fe₂O₃ NPs	727:735	Fe₂O₃ NPs	727:735	The FT-IR vibration peaks showed the formation of Fe₂O₃ NPs, and the vibration peak for Fe-O was 568 cm-1.
34167634	6	2	theme	sharp	852:856	arg1	peak					869:872	a sharp absorption peak	850:872	a sharp absorption peak at 219 nm	850:882	The broad absorption peak observed in the range of 250-350 nm and a sharp absorption peak at 219 nm represents the UV absorption of the synthesized hybrid composites.
34167634	6	3	theme	hybrid	932:937	arg1	composites					939:948	the synthesized hybrid composites	916:948	the synthesized hybrid composites	916:948	The broad absorption peak observed in the range of 250-350 nm and a sharp absorption peak at 219 nm represents the UV absorption of the synthesized hybrid composites.
34167634	11	4	theme	suitable	1703:1710	arg1	generation					1717:1726	the suitable next generation	1699:1726	the suitable next generation therapeutic potential factors for prevention and treatment of antibiotic-resistant pneumonia	1699:1819	In conclusion, we expect biomimicking systems combined with the effective antibacterial agent could be the suitable next generation therapeutic potential factors for prevention and treatment of antibiotic-resistant pneumonia.
34167634	4	5	theme	chitosan	667:674	arg1	surface					656:662	the surface	652:662	the surface of chitosan	652:674	The results showed that α-Fe₂O₃ and GdF hybrid nanocomposites were successfully grown on the surface of chitosan.
34167634	10	6	theme	potential	1458:1466	arg1	action					1468:1473	the potential action	1454:1473	the potential action to effective inhibition ability on antibiotic-resistant P. aeruginosa, which has been main factor of inducing pneumonia	1454:1593	The in vivo bio inspired nanotherapeutics have the potential action to effective inhibition ability on antibiotic-resistant P. aeruginosa, which has been main factor of inducing pneumonia.
34167634	2	7	theme	external	337:344	arg1	agents					358:363	external stabilizing agents	337:363	external stabilizing agents	337:363	Unlike other methods, the addition of external stabilizing agents to generate GdF nanoparticles (NPs) was not necessary herein.
34167634	6	8	from	nm	881:882	arg1	peak					805:808	The broad absorption peak	784:808	The broad absorption peak observed in the range of 250-350 nm	784:844	The broad absorption peak observed in the range of 250-350 nm and a sharp absorption peak at 219 nm represents the UV absorption of the synthesized hybrid composites.
34167634	6	8	from	nm	881:882	arg1	peak					869:872	a sharp absorption peak	850:872	a sharp absorption peak at 219 nm	850:882	The broad absorption peak observed in the range of 250-350 nm and a sharp absorption peak at 219 nm represents the UV absorption of the synthesized hybrid composites.
34167634	10	9	theme	inhibition	1488:1497	arg1	ability					1499:1505	effective inhibition ability	1478:1505	effective inhibition ability	1478:1505	The in vivo bio inspired nanotherapeutics have the potential action to effective inhibition ability on antibiotic-resistant P. aeruginosa, which has been main factor of inducing pneumonia.
34167634	1	10	theme	composite	241:249	arg1	method					291:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	Herein, we have designed and developed a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method.
34167634	11	11	theme	biomimicking	1621:1632	arg1	systems					1634:1640	biomimicking systems	1621:1640	biomimicking systems combined with the effective antibacterial agent	1621:1688	In conclusion, we expect biomimicking systems combined with the effective antibacterial agent could be the suitable next generation therapeutic potential factors for prevention and treatment of antibiotic-resistant pneumonia.
34167634	2	12	theme	agents	358:363	arg1	addition					325:332	the addition	321:332	the addition of external stabilizing agents to generate GdF nanoparticles (NPs)	321:399	Unlike other methods, the addition of external stabilizing agents to generate GdF nanoparticles (NPs) was not necessary herein.
34167634	2	12	theme	agents	358:363	arg1	necessary					409:417	necessary	409:417	necessary	409:417	Unlike other methods, the addition of external stabilizing agents to generate GdF nanoparticles (NPs) was not necessary herein.
34167634	9	13	theme	hybrid	1320:1325	arg1	samples					1327:1333	developed hybrid samples	1310:1333	developed hybrid samples	1310:1333	In addition, in vitro biocompatibility results exhibited that developed hybrid samples have provided high cell compatibility with fibroblast (L929) cell line.
34167634	1	14	theme	chemical	268:275	arg1	method					291:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	Herein, we have designed and developed a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method.
34167634	9	15	theme	high	1349:1352	arg1	compatibility					1359:1371	high cell compatibility	1349:1371	high cell compatibility with fibroblast (L929) cell line	1349:1404	In addition, in vitro biocompatibility results exhibited that developed hybrid samples have provided high cell compatibility with fibroblast (L929) cell line.
34167634	7	16	theme	nanoparticles	1021:1033	arg1	crystallinity					985:997	crystallinity	985:997	crystallinity	985:997	XRD pattern showed sharp peaks of crystallinity and purity of α-Fe₂O₃ nanoparticles.
34167634	7	16	theme	nanoparticles	1021:1033	arg1	purity					1003:1008	purity	1003:1008	purity	1003:1008	XRD pattern showed sharp peaks of crystallinity and purity of α-Fe₂O₃ nanoparticles.
34167634	1	17	theme	heteromultivalent	174:190	arg1	method					291:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	Herein, we have designed and developed a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method.
34167634	0	18	theme	Bacterial	112:120	arg1	Pneumonia					122:130	Enhanced Antibiotic-Resistant Bacterial Pneumonia	82:130	Enhanced Antibiotic-Resistant Bacterial Pneumonia	82:130	Bioinspired Heteromultivalent Chitosan-α-Fe₂O₃/Gadofullerene Hybrid Composite for Enhanced Antibiotic-Resistant Bacterial Pneumonia.
34167634	2	19	theme	other	306:310	arg1	methods					312:318	other methods	306:318	other methods	306:318	Unlike other methods, the addition of external stabilizing agents to generate GdF nanoparticles (NPs) was not necessary herein.
34167634	1	20	theme	base	201:204	arg1	method					291:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	Herein, we have designed and developed a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method.
34167634	9	21	theme	fibroblast	1378:1387	arg1	line					1401:1404	fibroblast (L929) cell line	1378:1404	fibroblast (L929) cell line	1378:1404	In addition, in vitro biocompatibility results exhibited that developed hybrid samples have provided high cell compatibility with fibroblast (L929) cell line.
34167634	8	22	theme	chitosan-α-Fe₂O₃/GdF	1061:1080	arg1	composites					1089:1098	the synthesized chitosan-α-Fe₂O₃/GdF hybrid composites	1045:1098	the synthesized chitosan-α-Fe₂O₃/GdF hybrid composites	1045:1098	Finally, the synthesized chitosan-α-Fe₂O₃/GdF hybrid composites were screened for their antibacterial resistance against the Escherichia coli, Pseudomonas aeruginosa, Bacilus subtilis, and Staphylococcus aereus.
34167634	9	23	theme	L929	1390:1393	arg1	line					1401:1404	fibroblast (L929) cell line	1378:1404	fibroblast (L929) cell line	1378:1404	In addition, in vitro biocompatibility results exhibited that developed hybrid samples have provided high cell compatibility with fibroblast (L929) cell line.
34167634	5	24	theme	FT-IR	681:685	arg1	peaks					697:701	The FT-IR vibration peaks	677:701	The FT-IR vibration peaks	677:701	The FT-IR vibration peaks showed the formation of Fe₂O₃ NPs, and the vibration peak for Fe-O was 568 cm-1.
34167634	10	25	theme	main	1561:1564	arg1	factor					1566:1571	main factor	1561:1571	main factor of inducing pneumonia	1561:1593	The in vivo bio inspired nanotherapeutics have the potential action to effective inhibition ability on antibiotic-resistant P. aeruginosa, which has been main factor of inducing pneumonia.
34167634	9	26	theme	cell	1396:1399	arg1	line					1401:1404	fibroblast (L929) cell line	1378:1404	fibroblast (L929) cell line	1378:1404	In addition, in vitro biocompatibility results exhibited that developed hybrid samples have provided high cell compatibility with fibroblast (L929) cell line.
34167634	7	27	theme	sharp	970:974	arg1	peaks					976:980	sharp peaks	970:980	sharp peaks of crystallinity and purity of α-Fe₂O₃ nanoparticles	970:1033	XRD pattern showed sharp peaks of crystallinity and purity of α-Fe₂O₃ nanoparticles.
34167634	3	28	theme	prepared	431:438	arg1	nanocomposites					468:481	The prepared chitosan-α-Fe₂O₃/GdF hybrid nanocomposites	427:481	The prepared chitosan-α-Fe₂O₃/GdF hybrid nanocomposites	427:481	The prepared chitosan-α-Fe₂O₃/GdF hybrid nanocomposites were characterized using UV, FT-IR, XRD and morphological microscopic analyses.
34167634	11	29	theme	effective	1660:1668	arg1	agent					1684:1688	the effective antibacterial agent	1656:1688	the effective antibacterial agent	1656:1688	In conclusion, we expect biomimicking systems combined with the effective antibacterial agent could be the suitable next generation therapeutic potential factors for prevention and treatment of antibiotic-resistant pneumonia.
34167634	10	30	theme	pneumonia	1585:1593	arg1	factor					1566:1571	main factor	1561:1571	main factor of inducing pneumonia	1561:1593	The in vivo bio inspired nanotherapeutics have the potential action to effective inhibition ability on antibiotic-resistant P. aeruginosa, which has been main factor of inducing pneumonia.
34167634	9	31	theme	in	1261:1262	arg1	results					1287:1293	in vitro biocompatibility results	1261:1293	in vitro biocompatibility results	1261:1293	In addition, in vitro biocompatibility results exhibited that developed hybrid samples have provided high cell compatibility with fibroblast (L929) cell line.
34167634	0	32	theme	Chitosan-α-Fe₂O₃/Gadofullerene	30:59	arg1	Composite					68:76	Heteromultivalent Chitosan-α-Fe₂O₃/Gadofullerene Hybrid Composite	12:76	Heteromultivalent Chitosan-α-Fe₂O₃/Gadofullerene Hybrid Composite for Enhanced Antibiotic-Resistant Bacterial Pneumonia	12:130	Bioinspired Heteromultivalent Chitosan-α-Fe₂O₃/Gadofullerene Hybrid Composite for Enhanced Antibiotic-Resistant Bacterial Pneumonia.
34167634	6	33	theme	UV	899:900	arg1	absorption					902:911	the UV absorption	895:911	the UV absorption of the synthesized hybrid composites	895:948	The broad absorption peak observed in the range of 250-350 nm and a sharp absorption peak at 219 nm represents the UV absorption of the synthesized hybrid composites.
34167634	6	34	located	observed	810:817	arg2	peak					805:808	The broad absorption peak	784:808	The broad absorption peak observed in the range of 250-350 nm	784:844	The broad absorption peak observed in the range of 250-350 nm and a sharp absorption peak at 219 nm represents the UV absorption of the synthesized hybrid composites.
34167634	6	34	located	observed	810:817	arg1	range					826:830	the range	822:830	the range of 250-350 nm	822:844	The broad absorption peak observed in the range of 250-350 nm and a sharp absorption peak at 219 nm represents the UV absorption of the synthesized hybrid composites.
34167634	3	35	theme	hybrid	461:466	arg1	nanocomposites					468:481	The prepared chitosan-α-Fe₂O₃/GdF hybrid nanocomposites	427:481	The prepared chitosan-α-Fe₂O₃/GdF hybrid nanocomposites	427:481	The prepared chitosan-α-Fe₂O₃/GdF hybrid nanocomposites were characterized using UV, FT-IR, XRD and morphological microscopic analyses.
34167634	11	36	theme	pneumonia	1811:1819	arg1	treatment					1777:1785	treatment	1777:1785	treatment	1777:1785	In conclusion, we expect biomimicking systems combined with the effective antibacterial agent could be the suitable next generation therapeutic potential factors for prevention and treatment of antibiotic-resistant pneumonia.
34167634	11	36	theme	pneumonia	1811:1819	arg1	prevention					1762:1771	prevention	1762:1771	prevention	1762:1771	In conclusion, we expect biomimicking systems combined with the effective antibacterial agent could be the suitable next generation therapeutic potential factors for prevention and treatment of antibiotic-resistant pneumonia.
34167634	10	37	theme	in	1411:1412	arg1	nanotherapeutics					1432:1447	The in vivo bio inspired nanotherapeutics	1407:1447	The in vivo bio inspired nanotherapeutics	1407:1447	The in vivo bio inspired nanotherapeutics have the potential action to effective inhibition ability on antibiotic-resistant P. aeruginosa, which has been main factor of inducing pneumonia.
34167634	0	38	theme	Enhanced	82:89	arg1	Pneumonia					122:130	Enhanced Antibiotic-Resistant Bacterial Pneumonia	82:130	Enhanced Antibiotic-Resistant Bacterial Pneumonia	82:130	Bioinspired Heteromultivalent Chitosan-α-Fe₂O₃/Gadofullerene Hybrid Composite for Enhanced Antibiotic-Resistant Bacterial Pneumonia.
34167634	4	39	theme	hybrid	603:608	arg1	nanocomposites					610:623	GdF hybrid nanocomposites	599:623	GdF hybrid nanocomposites	599:623	The results showed that α-Fe₂O₃ and GdF hybrid nanocomposites were successfully grown on the surface of chitosan.
34167634	6	40	theme	absorption	794:803	arg1	peak					805:808	The broad absorption peak	784:808	The broad absorption peak observed in the range of 250-350 nm	784:844	The broad absorption peak observed in the range of 250-350 nm and a sharp absorption peak at 219 nm represents the UV absorption of the synthesized hybrid composites.
34167634	3	41	theme	microscopic	541:551	arg1	analyses					553:560	morphological microscopic analyses	527:560	morphological microscopic analyses	527:560	The prepared chitosan-α-Fe₂O₃/GdF hybrid nanocomposites were characterized using UV, FT-IR, XRD and morphological microscopic analyses.
34167634	7	42	theme	XRD	951:953	arg1	pattern					955:961	XRD pattern	951:961	XRD pattern	951:961	XRD pattern showed sharp peaks of crystallinity and purity of α-Fe₂O₃ nanoparticles.
34167634	1	43	theme	precipitation	277:289	arg1	method					291:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	Herein, we have designed and developed a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method.
34167634	10	44	from	action	1468:1473	arg1	aeruginosa					1534:1543	P. aeruginosa	1531:1543	antibiotic-resistant P. aeruginosa	1510:1543	The in vivo bio inspired nanotherapeutics have the potential action to effective inhibition ability on antibiotic-resistant P. aeruginosa, which has been main factor of inducing pneumonia.
34167634	11	45	theme	generation	1717:1726	arg1	factors					1750:1756	the suitable next generation therapeutic potential factors	1699:1756	the suitable next generation therapeutic potential factors for prevention and treatment of antibiotic-resistant pneumonia	1699:1819	In conclusion, we expect biomimicking systems combined with the effective antibacterial agent could be the suitable next generation therapeutic potential factors for prevention and treatment of antibiotic-resistant pneumonia.
34167634	10	46	theme	bio	1419:1421	arg1	nanotherapeutics					1432:1447	The in vivo bio inspired nanotherapeutics	1407:1447	The in vivo bio inspired nanotherapeutics	1407:1447	The in vivo bio inspired nanotherapeutics have the potential action to effective inhibition ability on antibiotic-resistant P. aeruginosa, which has been main factor of inducing pneumonia.
34167634	6	47	theme	composites	939:948	arg1	absorption					902:911	the UV absorption	895:911	the UV absorption of the synthesized hybrid composites	895:948	The broad absorption peak observed in the range of 250-350 nm and a sharp absorption peak at 219 nm represents the UV absorption of the synthesized hybrid composites.
34167634	11	48	theme	potential	1740:1748	arg1	factors					1750:1756	the suitable next generation therapeutic potential factors	1699:1756	the suitable next generation therapeutic potential factors for prevention and treatment of antibiotic-resistant pneumonia	1699:1819	In conclusion, we expect biomimicking systems combined with the effective antibacterial agent could be the suitable next generation therapeutic potential factors for prevention and treatment of antibiotic-resistant pneumonia.
34167634	5	49	theme	NPs	733:735	arg1	formation					714:722	the formation	710:722	the formation of Fe₂O₃ NPs	710:735	The FT-IR vibration peaks showed the formation of Fe₂O₃ NPs, and the vibration peak for Fe-O was 568 cm-1.
34167634	2	50	theme	stabilizing	346:356	arg1	agents					358:363	external stabilizing agents	337:363	external stabilizing agents	337:363	Unlike other methods, the addition of external stabilizing agents to generate GdF nanoparticles (NPs) was not necessary herein.
34167634	6	51	theme	synthesized	920:930	arg1	composites					939:948	the synthesized hybrid composites	916:948	the synthesized hybrid composites	916:948	The broad absorption peak observed in the range of 250-350 nm and a sharp absorption peak at 219 nm represents the UV absorption of the synthesized hybrid composites.
34167634	6	52	theme	nm	843:844	arg1	range					826:830	the range	822:830	the range of 250-350 nm	822:844	The broad absorption peak observed in the range of 250-350 nm and a sharp absorption peak at 219 nm represents the UV absorption of the synthesized hybrid composites.
34167634	1	53	theme	GdF	229:231	arg1	method					291:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	Herein, we have designed and developed a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method.
34167634	9	54	theme	biocompatibility	1270:1285	arg1	results					1287:1293	in vitro biocompatibility results	1261:1293	in vitro biocompatibility results	1261:1293	In addition, in vitro biocompatibility results exhibited that developed hybrid samples have provided high cell compatibility with fibroblast (L929) cell line.
34167634	2	55	theme	GdF	377:379	arg1	NPs					396:398	NPs	396:398	NPs	396:398	Unlike other methods, the addition of external stabilizing agents to generate GdF nanoparticles (NPs) was not necessary herein.
34167634	2	55	theme	GdF	377:379	arg1	nanoparticles					381:393	GdF nanoparticles	377:393	GdF nanoparticles (NPs)	377:399	Unlike other methods, the addition of external stabilizing agents to generate GdF nanoparticles (NPs) was not necessary herein.
34167634	1	56	theme	hybrid	234:239	arg1	method					291:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	Herein, we have designed and developed a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method.
34167634	8	57	theme	synthesized	1049:1059	arg1	composites					1089:1098	the synthesized chitosan-α-Fe₂O₃/GdF hybrid composites	1045:1098	the synthesized chitosan-α-Fe₂O₃/GdF hybrid composites	1045:1098	Finally, the synthesized chitosan-α-Fe₂O₃/GdF hybrid composites were screened for their antibacterial resistance against the Escherichia coli, Pseudomonas aeruginosa, Bacilus subtilis, and Staphylococcus aereus.
34167634	10	58	theme	effective	1478:1486	arg1	ability					1499:1505	effective inhibition ability	1478:1505	effective inhibition ability	1478:1505	The in vivo bio inspired nanotherapeutics have the potential action to effective inhibition ability on antibiotic-resistant P. aeruginosa, which has been main factor of inducing pneumonia.
34167634	1	59	theme	through	251:257	arg1	method					291:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	Herein, we have designed and developed a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method.
34167634	8	60	dep	Escherichia	1161:1171	arg1	coli					1173:1176	coli	1173:1176	coli	1173:1176	Finally, the synthesized chitosan-α-Fe₂O₃/GdF hybrid composites were screened for their antibacterial resistance against the Escherichia coli, Pseudomonas aeruginosa, Bacilus subtilis, and Staphylococcus aereus.
34167634	9	61	theme	developed	1310:1318	arg1	samples					1327:1333	developed hybrid samples	1310:1333	developed hybrid samples	1310:1333	In addition, in vitro biocompatibility results exhibited that developed hybrid samples have provided high cell compatibility with fibroblast (L929) cell line.
34167634	10	62	dep	in	1411:1412	arg1	vivo					1414:1417	vivo	1414:1417	vivo	1414:1417	The in vivo bio inspired nanotherapeutics have the potential action to effective inhibition ability on antibiotic-resistant P. aeruginosa, which has been main factor of inducing pneumonia.
34167634	1	63	theme	simple	261:266	arg1	method					291:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	Herein, we have designed and developed a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method.
34167634	5	64	theme	vibration	746:754	arg1	cm-1					778:781	568 cm-1	774:781	568 cm-1	774:781	The FT-IR vibration peaks showed the formation of Fe₂O₃ NPs, and the vibration peak for Fe-O was 568 cm-1.
34167634	5	64	theme	vibration	746:754	arg1	peak					756:759	the vibration peak	742:759	the vibration peak for Fe-O	742:768	The FT-IR vibration peaks showed the formation of Fe₂O₃ NPs, and the vibration peak for Fe-O was 568 cm-1.
34167634	9	65	dep	in	1261:1262	arg1	vitro					1264:1268	vitro	1264:1268	vitro	1264:1268	In addition, in vitro biocompatibility results exhibited that developed hybrid samples have provided high cell compatibility with fibroblast (L929) cell line.
34167634	9	66	theme	cell	1354:1357	arg1	compatibility					1359:1371	high cell compatibility	1349:1371	high cell compatibility with fibroblast (L929) cell line	1349:1404	In addition, in vitro biocompatibility results exhibited that developed hybrid samples have provided high cell compatibility with fibroblast (L929) cell line.
34167634	1	67	theme	chitosan	192:199	arg1	method					291:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	Herein, we have designed and developed a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method.
34167634	8	68	theme	hybrid	1082:1087	arg1	composites					1089:1098	the synthesized chitosan-α-Fe₂O₃/GdF hybrid composites	1045:1098	the synthesized chitosan-α-Fe₂O₃/GdF hybrid composites	1045:1098	Finally, the synthesized chitosan-α-Fe₂O₃/GdF hybrid composites were screened for their antibacterial resistance against the Escherichia coli, Pseudomonas aeruginosa, Bacilus subtilis, and Staphylococcus aereus.
34167634	7	69	theme	α-Fe₂O₃	1013:1019	arg1	nanoparticles					1021:1033	α-Fe₂O₃ nanoparticles	1013:1033	α-Fe₂O₃ nanoparticles	1013:1033	XRD pattern showed sharp peaks of crystallinity and purity of α-Fe₂O₃ nanoparticles.
34167634	1	70	theme	α-Fe₂O₃/Gadofullerene	206:226	arg1	method					291:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method	172:296	Herein, we have designed and developed a heteromultivalent chitosan base α-Fe₂O₃/Gadofullerene (GdF) hybrid composite through a simple chemical precipitation method.
34167634	9	71	with	compatibility	1359:1371	arg1	line					1401:1404	fibroblast (L929) cell line	1378:1404	fibroblast (L929) cell line	1378:1404	In addition, in vitro biocompatibility results exhibited that developed hybrid samples have provided high cell compatibility with fibroblast (L929) cell line.
34167634	7	72	theme	purity	1003:1008	arg1	peaks					976:980	sharp peaks	970:980	sharp peaks of crystallinity and purity of α-Fe₂O₃ nanoparticles	970:1033	XRD pattern showed sharp peaks of crystallinity and purity of α-Fe₂O₃ nanoparticles.
34167634	11	73	theme	next	1712:1715	arg1	generation					1717:1726	the suitable next generation	1699:1726	the suitable next generation therapeutic potential factors for prevention and treatment of antibiotic-resistant pneumonia	1699:1819	In conclusion, we expect biomimicking systems combined with the effective antibacterial agent could be the suitable next generation therapeutic potential factors for prevention and treatment of antibiotic-resistant pneumonia.
34167634	5	74	theme	vibration	687:695	arg1	peaks					697:701	The FT-IR vibration peaks	677:701	The FT-IR vibration peaks	677:701	The FT-IR vibration peaks showed the formation of Fe₂O₃ NPs, and the vibration peak for Fe-O was 568 cm-1.
34167634	0	75	theme	Heteromultivalent	12:28	arg1	Composite					68:76	Heteromultivalent Chitosan-α-Fe₂O₃/Gadofullerene Hybrid Composite	12:76	Heteromultivalent Chitosan-α-Fe₂O₃/Gadofullerene Hybrid Composite for Enhanced Antibiotic-Resistant Bacterial Pneumonia	12:130	Bioinspired Heteromultivalent Chitosan-α-Fe₂O₃/Gadofullerene Hybrid Composite for Enhanced Antibiotic-Resistant Bacterial Pneumonia.
34167634	8	76	theme	antibacterial	1124:1136	arg1	resistance					1138:1147	their antibacterial resistance	1118:1147	their antibacterial resistance against the Escherichia coli, Pseudomonas aeruginosa, Bacilus subtilis, and Staphylococcus aereus	1118:1245	Finally, the synthesized chitosan-α-Fe₂O₃/GdF hybrid composites were screened for their antibacterial resistance against the Escherichia coli, Pseudomonas aeruginosa, Bacilus subtilis, and Staphylococcus aereus.
34167634	3	77	theme	chitosan-α-Fe₂O₃/GdF	440:459	arg1	nanocomposites					468:481	The prepared chitosan-α-Fe₂O₃/GdF hybrid nanocomposites	427:481	The prepared chitosan-α-Fe₂O₃/GdF hybrid nanocomposites	427:481	The prepared chitosan-α-Fe₂O₃/GdF hybrid nanocomposites were characterized using UV, FT-IR, XRD and morphological microscopic analyses.
34167634	0	78	theme	Hybrid	61:66	arg1	Composite					68:76	Heteromultivalent Chitosan-α-Fe₂O₃/Gadofullerene Hybrid Composite	12:76	Heteromultivalent Chitosan-α-Fe₂O₃/Gadofullerene Hybrid Composite for Enhanced Antibiotic-Resistant Bacterial Pneumonia	12:130	Bioinspired Heteromultivalent Chitosan-α-Fe₂O₃/Gadofullerene Hybrid Composite for Enhanced Antibiotic-Resistant Bacterial Pneumonia.
34167634	7	79	theme	crystallinity	985:997	arg1	peaks					976:980	sharp peaks	970:980	sharp peaks of crystallinity and purity of α-Fe₂O₃ nanoparticles	970:1033	XRD pattern showed sharp peaks of crystallinity and purity of α-Fe₂O₃ nanoparticles.
34167634	10	80	theme	inducing	1576:1583	arg1	pneumonia					1585:1593	inducing pneumonia	1576:1593	inducing pneumonia	1576:1593	The in vivo bio inspired nanotherapeutics have the potential action to effective inhibition ability on antibiotic-resistant P. aeruginosa, which has been main factor of inducing pneumonia.
34167634	3	81	theme	morphological	527:539	arg1	analyses					553:560	morphological microscopic analyses	527:560	morphological microscopic analyses	527:560	The prepared chitosan-α-Fe₂O₃/GdF hybrid nanocomposites were characterized using UV, FT-IR, XRD and morphological microscopic analyses.
34167634	4	82	theme	GdF	599:601	arg1	nanocomposites					610:623	GdF hybrid nanocomposites	599:623	GdF hybrid nanocomposites	599:623	The results showed that α-Fe₂O₃ and GdF hybrid nanocomposites were successfully grown on the surface of chitosan.
34167634	11	83	theme	antibiotic-resistant	1790:1809	arg1	pneumonia					1811:1819	antibiotic-resistant pneumonia	1790:1819	antibiotic-resistant pneumonia	1790:1819	In conclusion, we expect biomimicking systems combined with the effective antibacterial agent could be the suitable next generation therapeutic potential factors for prevention and treatment of antibiotic-resistant pneumonia.
34167634	0	84	theme	Antibiotic-Resistant	91:110	arg1	Pneumonia					122:130	Enhanced Antibiotic-Resistant Bacterial Pneumonia	82:130	Enhanced Antibiotic-Resistant Bacterial Pneumonia	82:130	Bioinspired Heteromultivalent Chitosan-α-Fe₂O₃/Gadofullerene Hybrid Composite for Enhanced Antibiotic-Resistant Bacterial Pneumonia.
34167634	11	85	theme	antibacterial	1670:1682	arg1	agent					1684:1688	the effective antibacterial agent	1656:1688	the effective antibacterial agent	1656:1688	In conclusion, we expect biomimicking systems combined with the effective antibacterial agent could be the suitable next generation therapeutic potential factors for prevention and treatment of antibiotic-resistant pneumonia.
34167634	10	86	contain	have	1449:1452	arg1	nanotherapeutics					1432:1447	The in vivo bio inspired nanotherapeutics	1407:1447	The in vivo bio inspired nanotherapeutics	1407:1447	The in vivo bio inspired nanotherapeutics have the potential action to effective inhibition ability on antibiotic-resistant P. aeruginosa, which has been main factor of inducing pneumonia.
34167634	10	86	contain	have	1449:1452	arg2	action					1468:1473	the potential action	1454:1473	the potential action to effective inhibition ability on antibiotic-resistant P. aeruginosa, which has been main factor of inducing pneumonia	1454:1593	The in vivo bio inspired nanotherapeutics have the potential action to effective inhibition ability on antibiotic-resistant P. aeruginosa, which has been main factor of inducing pneumonia.
34167634	6	87	theme	broad	788:792	arg1	peak					805:808	The broad absorption peak	784:808	The broad absorption peak observed in the range of 250-350 nm	784:844	The broad absorption peak observed in the range of 250-350 nm and a sharp absorption peak at 219 nm represents the UV absorption of the synthesized hybrid composites.
34167634	6	88	theme	absorption	858:867	arg1	peak					869:872	a sharp absorption peak	850:872	a sharp absorption peak at 219 nm	850:882	The broad absorption peak observed in the range of 250-350 nm and a sharp absorption peak at 219 nm represents the UV absorption of the synthesized hybrid composites.
34167634	10	89	theme	inspired	1423:1430	arg1	nanotherapeutics					1432:1447	The in vivo bio inspired nanotherapeutics	1407:1447	The in vivo bio inspired nanotherapeutics	1407:1447	The in vivo bio inspired nanotherapeutics have the potential action to effective inhibition ability on antibiotic-resistant P. aeruginosa, which has been main factor of inducing pneumonia.
33256116	8	0	located	detected	1374:1381	arg2	marker					1358:1363	the isolated marker	1345:1363	the isolated marker	1345:1363	At the highest level of specificity, the isolated marker was only detected in porcine heparin.
33256116	8	0	located	detected	1374:1381	arg1	heparin					1394:1400	porcine heparin	1386:1400	porcine heparin	1386:1400	At the highest level of specificity, the isolated marker was only detected in porcine heparin.
33256116	8	0	located	detected	1374:1381	arg1	level					1323:1327	the highest level	1311:1327	the highest level of specificity	1311:1342	At the highest level of specificity, the isolated marker was only detected in porcine heparin.
33256116	0	1	from	Ends	22:25	arg1	Origins					61:67	Different Animal Origins	44:67	Different Animal Origins	44:67	Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid.
33256116	0	1	from	Ends	22:25	arg1	Heparins					30:37	Heparins	30:37	Heparins from Different Animal Origins	30:67	Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid.
33256116	9	2	theme	heparin	1561:1567	arg1	types					1569:1573	heparin types	1561:1573	heparin types	1561:1573	However, the proportion of glucosamines in the NRE and the proportion of glucuronic/iduronic configurations in the NRE uronic moieties greatly varied between heparin types.
33256116	9	3	theme	glucosamines	1430:1441	arg1	proportion					1416:1425	the proportion	1412:1425	the proportion of glucosamines in the NRE	1412:1452	However, the proportion of glucosamines in the NRE and the proportion of glucuronic/iduronic configurations in the NRE uronic moieties greatly varied between heparin types.
33256116	9	3	theme	glucosamines	1430:1441	arg1	proportion					1462:1471	the proportion	1458:1471	the proportion of glucuronic/iduronic configurations in the NRE uronic moieties	1458:1536	However, the proportion of glucosamines in the NRE and the proportion of glucuronic/iduronic configurations in the NRE uronic moieties greatly varied between heparin types.
33256116	0	4	theme	Building	70:77	arg1	Blocks					79:84	Building Blocks	70:84	Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid.	0:147	Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid.
33256116	2	5	from	NRE	452:454	arg1	glucosamines					432:443	N-sulfated glucosamines	421:443	N-sulfated glucosamines at the NRE	421:454	Their nonreducing-end (NRE) has been little investigated due to challenges in their characterization, but is known to be partly generated by enzymatic cleavage with heparanases, resulting in N-sulfated glucosamines at the NRE.
33256116	5	6	from	chromatography	933:946	arg1	columns					956:962	AS11 columns	951:962	AS11 columns for the assay	951:976	This study describes a new method for identifying heparin classical building blocks and novel NRE building blocks using strong anion exchange chromatography on AS11 columns for the assay, and ion-pair liquid chromatography-mass spectrometry for building block identification.
33256116	1	7	theme	linear	162:167	arg1	Heparins					149:156	Heparins	149:156	Heparins	149:156	Heparins are linear sulfated polysaccharides widely used as anticoagulant drugs.
33256116	1	7	theme	linear	162:167	arg1	polysaccharides					178:192	linear sulfated polysaccharides	162:192	linear sulfated polysaccharides widely used as anticoagulant drugs	162:227	Heparins are linear sulfated polysaccharides widely used as anticoagulant drugs.
33256116	4	8	with	amination	745:753	arg1	acid					771:774	sulfanilic acid	760:774	sulfanilic acid	760:774	To further characterize NRE in heparinoids, a building block analysis involving exhaustive heparinase digestion and subsequent reductive amination with sulfanilic acid was performed.
33256116	7	9	theme	observed	1237:1244	arg1	composition					1246:1256	the observed composition	1233:1256	the observed composition of the NRE	1233:1267	Generally, NRE on these three heparins are highly sulfated moieties, particularly with 3-O sulfates, and the observed composition of the NRE is highly dependent on heparin origin.
33256116	7	9	theme	observed	1237:1244	arg1	dependent					1279:1287	dependent	1279:1287	dependent	1279:1287	Generally, NRE on these three heparins are highly sulfated moieties, particularly with 3-O sulfates, and the observed composition of the NRE is highly dependent on heparin origin.
33256116	5	10	theme	block	1045:1049	arg1	identification					1051:1064	building block identification	1036:1064	building block identification	1036:1064	This study describes a new method for identifying heparin classical building blocks and novel NRE building blocks using strong anion exchange chromatography on AS11 columns for the assay, and ion-pair liquid chromatography-mass spectrometry for building block identification.
33256116	5	11	theme	exchange	924:931	arg1	chromatography					933:946	strong anion exchange chromatography	911:946	strong anion exchange chromatography on AS11 columns for the assay	911:976	This study describes a new method for identifying heparin classical building blocks and novel NRE building blocks using strong anion exchange chromatography on AS11 columns for the assay, and ion-pair liquid chromatography-mass spectrometry for building block identification.
33256116	7	12	with	moieties	1187:1194	arg1	sulfates					1219:1226	3-O sulfates	1215:1226	3-O sulfates	1215:1226	Generally, NRE on these three heparins are highly sulfated moieties, particularly with 3-O sulfates, and the observed composition of the NRE is highly dependent on heparin origin.
33256116	8	13	theme	highest	1315:1321	arg1	level					1323:1327	the highest level	1311:1327	the highest level of specificity	1311:1342	At the highest level of specificity, the isolated marker was only detected in porcine heparin.
33256116	5	14	theme	chromatography-mass	999:1017	arg1	spectrometry					1019:1030	ion-pair liquid chromatography-mass spectrometry	983:1030	ion-pair liquid chromatography-mass spectrometry for building block identification	983:1064	This study describes a new method for identifying heparin classical building blocks and novel NRE building blocks using strong anion exchange chromatography on AS11 columns for the assay, and ion-pair liquid chromatography-mass spectrometry for building block identification.
33256116	5	15	theme	building	889:896	arg1	blocks					898:903	novel NRE building blocks	879:903	novel NRE building blocks	879:903	This study describes a new method for identifying heparin classical building blocks and novel NRE building blocks using strong anion exchange chromatography on AS11 columns for the assay, and ion-pair liquid chromatography-mass spectrometry for building block identification.
33256116	0	16	theme	Amination	111:119	arg1	Tagging					121:127	Reductive Amination Tagging	101:127	Reductive Amination Tagging by Sulfanilic Acid	101:146	Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid.
33256116	4	17	with	digestion	710:718	arg1	acid					771:774	sulfanilic acid	760:774	sulfanilic acid	760:774	To further characterize NRE in heparinoids, a building block analysis involving exhaustive heparinase digestion and subsequent reductive amination with sulfanilic acid was performed.
33256116	7	18	theme	3-O	1215:1217	arg1	sulfates					1219:1226	3-O sulfates	1215:1226	3-O sulfates	1215:1226	Generally, NRE on these three heparins are highly sulfated moieties, particularly with 3-O sulfates, and the observed composition of the NRE is highly dependent on heparin origin.
33256116	6	19	theme	intestine	1094:1102	arg1	heparins					1104:1111	Porcine, ovine, and bovine intestine heparins	1067:1111	Porcine, ovine, and bovine intestine heparins	1067:1111	Porcine, ovine, and bovine intestine heparins were analyzed.
33256116	3	20	theme	porcine	524:530	arg1	heparin					532:538	porcine heparin	524:538	porcine heparin	524:538	Uronic NRE (specifically glucuronic acids) have been isolated from porcine heparin, with GlcA-GlcNS,3S,6S identified as a porcine-specific NRE marker.
33256116	1	21	theme	sulfated	169:176	arg1	Heparins					149:156	Heparins	149:156	Heparins	149:156	Heparins are linear sulfated polysaccharides widely used as anticoagulant drugs.
33256116	1	21	theme	sulfated	169:176	arg1	polysaccharides					178:192	linear sulfated polysaccharides	162:192	linear sulfated polysaccharides widely used as anticoagulant drugs	162:227	Heparins are linear sulfated polysaccharides widely used as anticoagulant drugs.
33256116	0	22	theme	Reductive	101:109	arg1	Amination					111:119	Reductive Amination	101:119	Reductive Amination Tagging by Sulfanilic Acid	101:146	Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid.
33256116	8	23	theme	porcine	1386:1392	arg1	heparin					1394:1400	porcine heparin	1386:1400	porcine heparin	1386:1400	At the highest level of specificity, the isolated marker was only detected in porcine heparin.
33256116	5	24	theme	heparin	841:847	arg1	blocks					868:873	heparin classical building blocks	841:873	heparin classical building blocks	841:873	This study describes a new method for identifying heparin classical building blocks and novel NRE building blocks using strong anion exchange chromatography on AS11 columns for the assay, and ion-pair liquid chromatography-mass spectrometry for building block identification.
33256116	4	25	theme	building	654:661	arg1	block					663:667	a building block	652:667	a building block analysis involving exhaustive heparinase digestion and subsequent reductive amination with sulfanilic acid	652:774	To further characterize NRE in heparinoids, a building block analysis involving exhaustive heparinase digestion and subsequent reductive amination with sulfanilic acid was performed.
33256116	0	26	theme	Non-Reducing	9:20	arg1	Ends					22:25	Specific Non-Reducing Ends	0:25	Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid.	0:147	Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid.
33256116	7	27	theme	NRE	1265:1267	arg1	composition					1246:1256	the observed composition	1233:1256	the observed composition of the NRE	1233:1267	Generally, NRE on these three heparins are highly sulfated moieties, particularly with 3-O sulfates, and the observed composition of the NRE is highly dependent on heparin origin.
33256116	7	27	theme	NRE	1265:1267	arg1	dependent					1279:1287	dependent	1279:1287	dependent	1279:1287	Generally, NRE on these three heparins are highly sulfated moieties, particularly with 3-O sulfates, and the observed composition of the NRE is highly dependent on heparin origin.
33256116	0	28	dep	Blocks	79:84	arg1	Analysis					86:93	Analysis	86:93	Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid.	0:147	Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid.
33256116	9	29	theme	configurations	1496:1509	arg1	proportion					1416:1425	the proportion	1412:1425	the proportion of glucosamines in the NRE	1412:1452	However, the proportion of glucosamines in the NRE and the proportion of glucuronic/iduronic configurations in the NRE uronic moieties greatly varied between heparin types.
33256116	9	29	theme	configurations	1496:1509	arg1	proportion					1462:1471	the proportion	1458:1471	the proportion of glucuronic/iduronic configurations in the NRE uronic moieties	1458:1536	However, the proportion of glucosamines in the NRE and the proportion of glucuronic/iduronic configurations in the NRE uronic moieties greatly varied between heparin types.
33256116	0	30	theme	Specific	0:7	arg1	Ends					22:25	Specific Non-Reducing Ends	0:25	Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid.	0:147	Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid.
33256116	4	31	theme	sulfanilic	760:769	arg1	acid					771:774	sulfanilic acid	760:774	sulfanilic acid	760:774	To further characterize NRE in heparinoids, a building block analysis involving exhaustive heparinase digestion and subsequent reductive amination with sulfanilic acid was performed.
33256116	2	32	theme	enzymatic	371:379	arg1	cleavage					381:388	enzymatic cleavage	371:388	enzymatic cleavage with heparanases	371:405	Their nonreducing-end (NRE) has been little investigated due to challenges in their characterization, but is known to be partly generated by enzymatic cleavage with heparanases, resulting in N-sulfated glucosamines at the NRE.
33256116	5	33	theme	classical	849:857	arg1	blocks					868:873	heparin classical building blocks	841:873	heparin classical building blocks	841:873	This study describes a new method for identifying heparin classical building blocks and novel NRE building blocks using strong anion exchange chromatography on AS11 columns for the assay, and ion-pair liquid chromatography-mass spectrometry for building block identification.
33256116	7	34	from	NRE	1139:1141	arg1	heparins					1158:1165	these three heparins	1146:1165	these three heparins	1146:1165	Generally, NRE on these three heparins are highly sulfated moieties, particularly with 3-O sulfates, and the observed composition of the NRE is highly dependent on heparin origin.
33256116	8	35	theme	isolated	1349:1356	arg1	marker					1358:1363	the isolated marker	1345:1363	the isolated marker	1345:1363	At the highest level of specificity, the isolated marker was only detected in porcine heparin.
33256116	2	36	theme	N-sulfated	421:430	arg1	glucosamines					432:443	N-sulfated glucosamines	421:443	N-sulfated glucosamines at the NRE	421:454	Their nonreducing-end (NRE) has been little investigated due to challenges in their characterization, but is known to be partly generated by enzymatic cleavage with heparanases, resulting in N-sulfated glucosamines at the NRE.
33256116	5	37	theme	building	859:866	arg1	blocks					868:873	heparin classical building blocks	841:873	heparin classical building blocks	841:873	This study describes a new method for identifying heparin classical building blocks and novel NRE building blocks using strong anion exchange chromatography on AS11 columns for the assay, and ion-pair liquid chromatography-mass spectrometry for building block identification.
33256116	5	38	theme	ion-pair	983:990	arg1	spectrometry					1019:1030	ion-pair liquid chromatography-mass spectrometry	983:1030	ion-pair liquid chromatography-mass spectrometry for building block identification	983:1064	This study describes a new method for identifying heparin classical building blocks and novel NRE building blocks using strong anion exchange chromatography on AS11 columns for the assay, and ion-pair liquid chromatography-mass spectrometry for building block identification.
33256116	1	39	theme	anticoagulant	209:221	arg1	drugs					223:227	anticoagulant drugs	209:227	anticoagulant drugs	209:227	Heparins are linear sulfated polysaccharides widely used as anticoagulant drugs.
33256116	5	40	theme	liquid	992:997	arg1	spectrometry					1019:1030	ion-pair liquid chromatography-mass spectrometry	983:1030	ion-pair liquid chromatography-mass spectrometry for building block identification	983:1064	This study describes a new method for identifying heparin classical building blocks and novel NRE building blocks using strong anion exchange chromatography on AS11 columns for the assay, and ion-pair liquid chromatography-mass spectrometry for building block identification.
33256116	9	41	theme	uronic	1522:1527	arg1	moieties					1529:1536	the NRE uronic moieties	1514:1536	the NRE uronic moieties	1514:1536	However, the proportion of glucosamines in the NRE and the proportion of glucuronic/iduronic configurations in the NRE uronic moieties greatly varied between heparin types.
33256116	6	42	theme	bovine	1087:1092	arg1	intestine					1094:1102	bovine intestine	1087:1102	bovine intestine	1087:1102	Porcine, ovine, and bovine intestine heparins were analyzed.
33256116	5	43	theme	strong	911:916	arg1	chromatography					933:946	strong anion exchange chromatography	911:946	strong anion exchange chromatography on AS11 columns for the assay	911:976	This study describes a new method for identifying heparin classical building blocks and novel NRE building blocks using strong anion exchange chromatography on AS11 columns for the assay, and ion-pair liquid chromatography-mass spectrometry for building block identification.
33256116	5	44	theme	novel	879:883	arg1	blocks					898:903	novel NRE building blocks	879:903	novel NRE building blocks	879:903	This study describes a new method for identifying heparin classical building blocks and novel NRE building blocks using strong anion exchange chromatography on AS11 columns for the assay, and ion-pair liquid chromatography-mass spectrometry for building block identification.
33256116	4	45	theme	block	663:667	arg1	analysis					669:676	a building block analysis	652:676	a building block analysis involving exhaustive heparinase digestion and subsequent reductive amination with sulfanilic acid	652:774	To further characterize NRE in heparinoids, a building block analysis involving exhaustive heparinase digestion and subsequent reductive amination with sulfanilic acid was performed.
33256116	0	46	theme	Sulfanilic	132:141	arg1	Acid					143:146	Sulfanilic Acid	132:146	Sulfanilic Acid	132:146	Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid.
33256116	3	47	theme	Uronic	457:462	arg1	acids					493:497	specifically glucuronic acids	469:497	specifically glucuronic acids	469:497	Uronic NRE (specifically glucuronic acids) have been isolated from porcine heparin, with GlcA-GlcNS,3S,6S identified as a porcine-specific NRE marker.
33256116	3	47	theme	Uronic	457:462	arg1	NRE					464:466	Uronic NRE	457:466	Uronic NRE (specifically glucuronic acids)	457:498	Uronic NRE (specifically glucuronic acids) have been isolated from porcine heparin, with GlcA-GlcNS,3S,6S identified as a porcine-specific NRE marker.
33256116	0	48	theme	Animal	54:59	arg1	Origins					61:67	Different Animal Origins	44:67	Different Animal Origins	44:67	Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid.
33256116	5	49	theme	anion	918:922	arg1	chromatography					933:946	strong anion exchange chromatography	911:946	strong anion exchange chromatography on AS11 columns for the assay	911:976	This study describes a new method for identifying heparin classical building blocks and novel NRE building blocks using strong anion exchange chromatography on AS11 columns for the assay, and ion-pair liquid chromatography-mass spectrometry for building block identification.
33256116	9	50	from	proportion	1416:1425	arg1	moieties					1529:1536	the NRE uronic moieties	1514:1536	the NRE uronic moieties	1514:1536	However, the proportion of glucosamines in the NRE and the proportion of glucuronic/iduronic configurations in the NRE uronic moieties greatly varied between heparin types.
33256116	9	50	from	proportion	1416:1425	arg1	NRE					1450:1452	the NRE	1446:1452	the NRE	1446:1452	However, the proportion of glucosamines in the NRE and the proportion of glucuronic/iduronic configurations in the NRE uronic moieties greatly varied between heparin types.
33256116	0	51	from	Origins	61:67	arg1	Ends					22:25	Specific Non-Reducing Ends	0:25	Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid.	0:147	Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid.
33256116	0	51	from	Origins	61:67	arg1	Heparins					30:37	Heparins	30:37	Heparins from Different Animal Origins	30:67	Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid.
33256116	3	52	theme	porcine-specific	579:594	arg1	marker					600:605	a porcine-specific NRE marker	577:605	a porcine-specific NRE marker	577:605	Uronic NRE (specifically glucuronic acids) have been isolated from porcine heparin, with GlcA-GlcNS,3S,6S identified as a porcine-specific NRE marker.
33256116	3	53	attach	isolated	510:517	arg2	NRE					464:466	Uronic NRE	457:466	Uronic NRE (specifically glucuronic acids)	457:498	Uronic NRE (specifically glucuronic acids) have been isolated from porcine heparin, with GlcA-GlcNS,3S,6S identified as a porcine-specific NRE marker.
33256116	3	53	attach	isolated	510:517	arg1	heparin					532:538	porcine heparin	524:538	porcine heparin	524:538	Uronic NRE (specifically glucuronic acids) have been isolated from porcine heparin, with GlcA-GlcNS,3S,6S identified as a porcine-specific NRE marker.
33256116	3	53	attach	isolated	510:517	arg2	acids					493:497	specifically glucuronic acids	469:497	specifically glucuronic acids	469:497	Uronic NRE (specifically glucuronic acids) have been isolated from porcine heparin, with GlcA-GlcNS,3S,6S identified as a porcine-specific NRE marker.
33256116	4	54	theme	reductive	735:743	arg1	amination					745:753	subsequent reductive amination	724:753	subsequent reductive amination	724:753	To further characterize NRE in heparinoids, a building block analysis involving exhaustive heparinase digestion and subsequent reductive amination with sulfanilic acid was performed.
33256116	0	55	theme	Different	44:52	arg1	Origins					61:67	Different Animal Origins	44:67	Different Animal Origins	44:67	Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid.
33256116	3	56	theme	NRE	596:598	arg1	marker					600:605	a porcine-specific NRE marker	577:605	a porcine-specific NRE marker	577:605	Uronic NRE (specifically glucuronic acids) have been isolated from porcine heparin, with GlcA-GlcNS,3S,6S identified as a porcine-specific NRE marker.
33256116	9	57	theme	NRE	1518:1520	arg1	moieties					1529:1536	the NRE uronic moieties	1514:1536	the NRE uronic moieties	1514:1536	However, the proportion of glucosamines in the NRE and the proportion of glucuronic/iduronic configurations in the NRE uronic moieties greatly varied between heparin types.
33256116	4	58	theme	subsequent	724:733	arg1	amination					745:753	subsequent reductive amination	724:753	subsequent reductive amination	724:753	To further characterize NRE in heparinoids, a building block analysis involving exhaustive heparinase digestion and subsequent reductive amination with sulfanilic acid was performed.
33256116	7	59	theme	heparin	1292:1298	arg1	origin					1300:1305	heparin origin	1292:1305	heparin origin	1292:1305	Generally, NRE on these three heparins are highly sulfated moieties, particularly with 3-O sulfates, and the observed composition of the NRE is highly dependent on heparin origin.
33256116	5	60	theme	building	1036:1043	arg1	block					1045:1049	building block	1036:1049	building block identification	1036:1064	This study describes a new method for identifying heparin classical building blocks and novel NRE building blocks using strong anion exchange chromatography on AS11 columns for the assay, and ion-pair liquid chromatography-mass spectrometry for building block identification.
33256116	8	61	theme	specificity	1332:1342	arg1	level					1323:1327	the highest level	1311:1327	the highest level of specificity	1311:1342	At the highest level of specificity, the isolated marker was only detected in porcine heparin.
33256116	5	62	theme	AS11	951:954	arg1	columns					956:962	AS11 columns	951:962	AS11 columns for the assay	951:976	This study describes a new method for identifying heparin classical building blocks and novel NRE building blocks using strong anion exchange chromatography on AS11 columns for the assay, and ion-pair liquid chromatography-mass spectrometry for building block identification.
33256116	0	63	dep	Ends	22:25	arg1	Blocks					79:84	Building Blocks	70:84	Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid.	0:147	Specific Non-Reducing Ends in Heparins from Different Animal Origins: Building Blocks Analysis Using Reductive Amination Tagging by Sulfanilic Acid.
33256116	9	64	from	proportion	1462:1471	arg1	moieties					1529:1536	the NRE uronic moieties	1514:1536	the NRE uronic moieties	1514:1536	However, the proportion of glucosamines in the NRE and the proportion of glucuronic/iduronic configurations in the NRE uronic moieties greatly varied between heparin types.
33256116	9	64	from	proportion	1462:1471	arg1	NRE					1450:1452	the NRE	1446:1452	the NRE	1446:1452	However, the proportion of glucosamines in the NRE and the proportion of glucuronic/iduronic configurations in the NRE uronic moieties greatly varied between heparin types.
33256116	3	65	theme	glucuronic	482:491	arg1	acids					493:497	specifically glucuronic acids	469:497	specifically glucuronic acids	469:497	Uronic NRE (specifically glucuronic acids) have been isolated from porcine heparin, with GlcA-GlcNS,3S,6S identified as a porcine-specific NRE marker.
33256116	3	65	theme	glucuronic	482:491	arg1	NRE					464:466	Uronic NRE	457:466	Uronic NRE (specifically glucuronic acids)	457:498	Uronic NRE (specifically glucuronic acids) have been isolated from porcine heparin, with GlcA-GlcNS,3S,6S identified as a porcine-specific NRE marker.
33256116	2	66	from	challenges	294:303	arg1	characterization					314:329	their characterization	308:329	their characterization	308:329	Their nonreducing-end (NRE) has been little investigated due to challenges in their characterization, but is known to be partly generated by enzymatic cleavage with heparanases, resulting in N-sulfated glucosamines at the NRE.
33256116	6	67	theme	ovine	1076:1080	arg1	heparins					1104:1111	Porcine, ovine, and bovine intestine heparins	1067:1111	Porcine, ovine, and bovine intestine heparins	1067:1111	Porcine, ovine, and bovine intestine heparins were analyzed.
33256116	4	68	from	NRE	632:634	arg1	heparinoids					639:649	heparinoids	639:649	heparinoids	639:649	To further characterize NRE in heparinoids, a building block analysis involving exhaustive heparinase digestion and subsequent reductive amination with sulfanilic acid was performed.
33256116	5	69	theme	NRE	885:887	arg1	blocks					898:903	novel NRE building blocks	879:903	novel NRE building blocks	879:903	This study describes a new method for identifying heparin classical building blocks and novel NRE building blocks using strong anion exchange chromatography on AS11 columns for the assay, and ion-pair liquid chromatography-mass spectrometry for building block identification.
33256116	9	70	theme	glucuronic/iduronic	1476:1494	arg1	configurations					1496:1509	glucuronic/iduronic configurations	1476:1509	glucuronic/iduronic configurations	1476:1509	However, the proportion of glucosamines in the NRE and the proportion of glucuronic/iduronic configurations in the NRE uronic moieties greatly varied between heparin types.
33256116	4	71	theme	heparinase	699:708	arg1	digestion					710:718	exhaustive heparinase digestion	688:718	exhaustive heparinase digestion	688:718	To further characterize NRE in heparinoids, a building block analysis involving exhaustive heparinase digestion and subsequent reductive amination with sulfanilic acid was performed.
33256116	7	72	theme	sulfated	1178:1185	arg1	moieties					1187:1194	highly sulfated moieties	1171:1194	highly sulfated moieties	1171:1194	Generally, NRE on these three heparins are highly sulfated moieties, particularly with 3-O sulfates, and the observed composition of the NRE is highly dependent on heparin origin.
33256116	7	72	theme	sulfated	1178:1185	arg1	NRE					1139:1141	NRE	1139:1141	NRE on these three heparins	1139:1165	Generally, NRE on these three heparins are highly sulfated moieties, particularly with 3-O sulfates, and the observed composition of the NRE is highly dependent on heparin origin.
33256116	2	73	with	cleavage	381:388	arg1	heparanases					395:405	heparanases	395:405	heparanases	395:405	Their nonreducing-end (NRE) has been little investigated due to challenges in their characterization, but is known to be partly generated by enzymatic cleavage with heparanases, resulting in N-sulfated glucosamines at the NRE.
33256116	5	74	theme	new	814:816	arg1	method					818:823	a new method	812:823	a new method for identifying heparin classical building blocks and novel NRE building blocks using strong anion exchange chromatography on AS11 columns for the assay, and ion-pair liquid chromatography-mass spectrometry for building block identification	812:1064	This study describes a new method for identifying heparin classical building blocks and novel NRE building blocks using strong anion exchange chromatography on AS11 columns for the assay, and ion-pair liquid chromatography-mass spectrometry for building block identification.
33256116	4	75	theme	exhaustive	688:697	arg1	digestion					710:718	exhaustive heparinase digestion	688:718	exhaustive heparinase digestion	688:718	To further characterize NRE in heparinoids, a building block analysis involving exhaustive heparinase digestion and subsequent reductive amination with sulfanilic acid was performed.
33256116	5	76	from	spectrometry	1019:1030	arg1	columns					956:962	AS11 columns	951:962	AS11 columns for the assay	951:976	This study describes a new method for identifying heparin classical building blocks and novel NRE building blocks using strong anion exchange chromatography on AS11 columns for the assay, and ion-pair liquid chromatography-mass spectrometry for building block identification.
33256116	6	77	theme	Porcine	1067:1073	arg1	heparins					1104:1111	Porcine, ovine, and bovine intestine heparins	1067:1111	Porcine, ovine, and bovine intestine heparins	1067:1111	Porcine, ovine, and bovine intestine heparins were analyzed.
33570567	4	0	from	graviresponses	496:509	arg1	thaliana					526:533	Arabidopsis thaliana	514:533	Arabidopsis thaliana	514:533	We took advantage of the effect of brassinosteroids (BRs) on the two types of graviresponses in Arabidopsis thaliana hypocotyls to disentangle functions of cell wall polymers during etiolated shoot growth.
33570567	10	1	from	base	1596:1599	arg1	thickening					1578:1587	their uneven thickening	1565:1587	their uneven thickening at the base	1565:1599	We also found that EBL interferes with upright growth of hypocotyls through their uneven thickening at the base.
33570567	9	2	from	effect	1403:1408	arg1	gravitropism					1424:1435	gravitropism	1424:1435	gravitropism	1424:1435	The negative effect of mannans on gravitropism is a new function for this class of hemicelluloses.
33570567	4	3	theme	wall	579:582	arg1	polymers					584:591	cell wall polymers	574:591	cell wall polymers	574:591	We took advantage of the effect of brassinosteroids (BRs) on the two types of graviresponses in Arabidopsis thaliana hypocotyls to disentangle functions of cell wall polymers during etiolated shoot growth.
33570567	10	4	theme	upright	1528:1534	arg1	growth					1536:1541	upright growth	1528:1541	upright growth of hypocotyls	1528:1555	We also found that EBL interferes with upright growth of hypocotyls through their uneven thickening at the base.
33570567	4	5	theme	graviresponses	496:509	arg1	types					487:491	the two types	479:491	the two types of graviresponses in Arabidopsis thaliana	479:533	We took advantage of the effect of brassinosteroids (BRs) on the two types of graviresponses in Arabidopsis thaliana hypocotyls to disentangle functions of cell wall polymers during etiolated shoot growth.
33570567	5	6	theme	seedlings	661:669	arg1	ability					628:634	The ability	624:634	The ability of etiolated Arabidopsis seedlings to grow upward	624:684	The ability of etiolated Arabidopsis seedlings to grow upward was suppressed in the presence of 24-epibrassinolide (EBL) but enhanced in the presence of brassinazole (BRZ), an inhibitor of BR biosynthesis.
33570567	7	7	theme	confocal	944:951	arg1	microscopy					953:962	confocal microscopy	944:962	confocal microscopy of the cellulose-specific pontamine S4B dye	944:1006	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	4	8	theme	cell	574:577	arg1	polymers					584:591	cell wall polymers	574:591	cell wall polymers	574:591	We took advantage of the effect of brassinosteroids (BRs) on the two types of graviresponses in Arabidopsis thaliana hypocotyls to disentangle functions of cell wall polymers during etiolated shoot growth.
33570567	6	9	from	changes	864:870	arg1	mechanics					885:893	cell wall mechanics	875:893	cell wall mechanics	875:893	These effects were accompanied by changes in cell wall mechanics and composition.
33570567	6	9	from	changes	864:870	arg1	composition					899:909	composition	899:909	composition	899:909	These effects were accompanied by changes in cell wall mechanics and composition.
33570567	6	10	theme	wall	880:883	arg1	mechanics					885:893	cell wall mechanics	875:893	cell wall mechanics	875:893	These effects were accompanied by changes in cell wall mechanics and composition.
33570567	4	11	from	types	487:491	arg1	thaliana					526:533	Arabidopsis thaliana	514:533	Arabidopsis thaliana	514:533	We took advantage of the effect of brassinosteroids (BRs) on the two types of graviresponses in Arabidopsis thaliana hypocotyls to disentangle functions of cell wall polymers during etiolated shoot growth.
33570567	4	11	from	types	487:491	arg1	advantage					426:434	advantage	426:434	advantage of the effect of brassinosteroids (BRs) on the two types of graviresponses in Arabidopsis thaliana	426:533	We took advantage of the effect of brassinosteroids (BRs) on the two types of graviresponses in Arabidopsis thaliana hypocotyls to disentangle functions of cell wall polymers during etiolated shoot growth.
33570567	8	12	theme	longitudinal	1202:1213	arg1	reorientation					1215:1227	a longitudinal reorientation	1200:1227	a longitudinal reorientation of cellulose fibres	1200:1247	Indeed, a longitudinal reorientation of cellulose fibres and growth inhibition at the base of hypocotyls supported their upright posture whereas the presence of mannans reduced gravitropic bending.
33570567	10	13	theme	uneven	1571:1576	arg1	thickening					1578:1587	their uneven thickening	1565:1587	their uneven thickening at the base	1565:1599	We also found that EBL interferes with upright growth of hypocotyls through their uneven thickening at the base.
33570567	6	14	theme	cell	875:878	arg1	mechanics					885:893	cell wall mechanics	875:893	cell wall mechanics	875:893	These effects were accompanied by changes in cell wall mechanics and composition.
33570567	7	15	theme	cellulose	1105:1113	arg1	organization					1121:1132	cellulose fibre organization	1105:1132	cellulose fibre organization	1105:1132	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	4	16	theme	polymers	584:591	arg1	functions					561:569	functions	561:569	functions of cell wall polymers	561:591	We took advantage of the effect of brassinosteroids (BRs) on the two types of graviresponses in Arabidopsis thaliana hypocotyls to disentangle functions of cell wall polymers during etiolated shoot growth.
33570567	7	17	theme	mannan	1176:1181	arg1	content					1183:1189	mannan content	1176:1189	mannan content	1176:1189	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	2	18	theme	Plant	163:167	arg1	shoots					169:174	Plant shoots	163:174	Plant shoots	163:174	Plant shoots respond to gravity through negative gravitropism and gravity resistance.
33570567	8	19	theme	growth	1253:1258	arg1	inhibition					1260:1269	growth inhibition	1253:1269	growth inhibition at the base of hypocotyls	1253:1295	Indeed, a longitudinal reorientation of cellulose fibres and growth inhibition at the base of hypocotyls supported their upright posture whereas the presence of mannans reduced gravitropic bending.
33570567	8	20	theme	mannans	1353:1359	arg1	presence					1341:1348	the presence	1337:1348	the presence of mannans	1337:1359	Indeed, a longitudinal reorientation of cellulose fibres and growth inhibition at the base of hypocotyls supported their upright posture whereas the presence of mannans reduced gravitropic bending.
33570567	5	21	theme	brassinazole	777:788	arg1	presence					765:772	the presence	761:772	the presence of brassinazole (BRZ), an inhibitor of BR biosynthesis	761:827	The ability of etiolated Arabidopsis seedlings to grow upward was suppressed in the presence of 24-epibrassinolide (EBL) but enhanced in the presence of brassinazole (BRZ), an inhibitor of BR biosynthesis.
33570567	7	22	theme	cell	1135:1138	arg1	expansion					1140:1148	cell expansion	1135:1148	cell expansion at the hypocotyl base	1135:1170	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	5	23	theme	Arabidopsis	649:659	arg1	seedlings					661:669	etiolated Arabidopsis seedlings	639:669	etiolated Arabidopsis seedlings	639:669	The ability of etiolated Arabidopsis seedlings to grow upward was suppressed in the presence of 24-epibrassinolide (EBL) but enhanced in the presence of brassinazole (BRZ), an inhibitor of BR biosynthesis.
33570567	9	24	theme	mannans	1413:1419	arg1	effect					1403:1408	The negative effect	1390:1408	The negative effect of mannans on gravitropism	1390:1435	The negative effect of mannans on gravitropism is a new function for this class of hemicelluloses.
33570567	9	24	theme	mannans	1413:1419	arg1	function					1446:1453	a new function	1440:1453	a new function for this class of hemicelluloses	1440:1486	The negative effect of mannans on gravitropism is a new function for this class of hemicelluloses.
33570567	7	25	theme	wall	917:920	arg1	analyses					934:941	Cell wall biochemical analyses	912:941	Cell wall biochemical analyses	912:941	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	9	26	theme	negative	1394:1401	arg1	effect					1403:1408	The negative effect	1390:1408	The negative effect of mannans on gravitropism	1390:1435	The negative effect of mannans on gravitropism is a new function for this class of hemicelluloses.
33570567	9	26	theme	negative	1394:1401	arg1	function					1446:1453	a new function	1440:1453	a new function for this class of hemicelluloses	1440:1486	The negative effect of mannans on gravitropism is a new function for this class of hemicelluloses.
33570567	10	27	theme	hypocotyls	1546:1555	arg1	growth					1536:1541	upright growth	1528:1541	upright growth of hypocotyls	1528:1555	We also found that EBL interferes with upright growth of hypocotyls through their uneven thickening at the base.
33570567	9	28	theme	new	1442:1444	arg1	function					1446:1453	a new function	1440:1453	a new function for this class of hemicelluloses	1440:1486	The negative effect of mannans on gravitropism is a new function for this class of hemicelluloses.
33570567	9	28	theme	new	1442:1444	arg1	effect					1403:1408	The negative effect	1390:1408	The negative effect of mannans on gravitropism	1390:1435	The negative effect of mannans on gravitropism is a new function for this class of hemicelluloses.
33570567	3	29	theme	upright	388:394	arg1	posture					396:402	an upright posture	385:402	an upright posture above ground	385:415	These responses are essential for plants to direct the growth of aerial organs away from the soil surface after germination and to keep an upright posture above ground.
33570567	7	30	theme	biochemical	922:932	arg1	analyses					934:941	Cell wall biochemical analyses	912:941	Cell wall biochemical analyses	912:941	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	8	31	theme	gravitropic	1369:1379	arg1	bending					1381:1387	gravitropic bending	1369:1387	gravitropic bending	1369:1387	Indeed, a longitudinal reorientation of cellulose fibres and growth inhibition at the base of hypocotyls supported their upright posture whereas the presence of mannans reduced gravitropic bending.
33570567	0	32	from	Changes	72:78	arg1	Cellulose					95:103	Cellulose	95:103	Cellulose	95:103	Brassinosteroids Influence Arabidopsis Hypocotyl Graviresponses through Changes in Mannans and Cellulose.
33570567	0	32	from	Changes	72:78	arg1	Mannans					83:89	Mannans	83:89	Mannans	83:89	Brassinosteroids Influence Arabidopsis Hypocotyl Graviresponses through Changes in Mannans and Cellulose.
33570567	5	33	theme	etiolated	639:647	arg1	seedlings					661:669	etiolated Arabidopsis seedlings	639:669	etiolated Arabidopsis seedlings	639:669	The ability of etiolated Arabidopsis seedlings to grow upward was suppressed in the presence of 24-epibrassinolide (EBL) but enhanced in the presence of brassinazole (BRZ), an inhibitor of BR biosynthesis.
33570567	8	34	theme	fibres	1242:1247	arg1	inhibition					1260:1269	growth inhibition	1253:1269	growth inhibition at the base of hypocotyls	1253:1295	Indeed, a longitudinal reorientation of cellulose fibres and growth inhibition at the base of hypocotyls supported their upright posture whereas the presence of mannans reduced gravitropic bending.
33570567	8	34	theme	fibres	1242:1247	arg1	reorientation					1215:1227	a longitudinal reorientation	1200:1227	a longitudinal reorientation of cellulose fibres	1200:1247	Indeed, a longitudinal reorientation of cellulose fibres and growth inhibition at the base of hypocotyls supported their upright posture whereas the presence of mannans reduced gravitropic bending.
33570567	0	35	theme	Hypocotyl	39:47	arg1	Graviresponses					49:62	Arabidopsis Hypocotyl Graviresponses	27:62	Arabidopsis Hypocotyl Graviresponses	27:62	Brassinosteroids Influence Arabidopsis Hypocotyl Graviresponses through Changes in Mannans and Cellulose.
33570567	7	36	theme	fibre	1115:1119	arg1	organization					1121:1132	cellulose fibre organization	1105:1132	cellulose fibre organization	1105:1132	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	7	37	theme	Cell	912:915	arg1	analyses					934:941	Cell wall biochemical analyses	912:941	Cell wall biochemical analyses	912:941	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	7	38	from	base	1167:1170	arg1	expansion					1140:1148	cell expansion	1135:1148	cell expansion at the hypocotyl base	1135:1170	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	7	38	from	base	1167:1170	arg1	changes					1094:1100	changes	1094:1100	changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content	1094:1189	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	7	38	from	base	1167:1170	arg1	content					1183:1189	mannan content	1176:1189	mannan content	1176:1189	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	7	38	from	base	1167:1170	arg1	organization					1121:1132	cellulose fibre organization	1105:1132	cellulose fibre organization	1105:1132	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	7	39	theme	cellular	1012:1019	arg1	analyses					1028:1035	cellular growth analyses	1012:1035	cellular growth analyses	1012:1035	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	0	40	theme	Arabidopsis	27:37	arg1	Graviresponses					49:62	Arabidopsis Hypocotyl Graviresponses	27:62	Arabidopsis Hypocotyl Graviresponses	27:62	Brassinosteroids Influence Arabidopsis Hypocotyl Graviresponses through Changes in Mannans and Cellulose.
33570567	7	41	theme	BRZ	1063:1065	arg1	treatments					1067:1076	the EBL and BRZ treatments	1051:1076	treatments	1067:1076	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	1	42	theme	gravity	119:125	arg1	force					110:114	The force	106:114	The force of gravity	106:125	The force of gravity is a constant environmental factor.
33570567	1	42	theme	gravity	119:125	arg1	factor					155:160	a constant environmental factor	130:160	a constant environmental factor	130:160	The force of gravity is a constant environmental factor.
33570567	5	43	theme	BR	813:814	arg1	biosynthesis					816:827	BR biosynthesis	813:827	BR biosynthesis	813:827	The ability of etiolated Arabidopsis seedlings to grow upward was suppressed in the presence of 24-epibrassinolide (EBL) but enhanced in the presence of brassinazole (BRZ), an inhibitor of BR biosynthesis.
33570567	7	44	from	changes	1094:1100	arg1	expansion					1140:1148	cell expansion	1135:1148	cell expansion at the hypocotyl base	1135:1170	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	7	44	from	changes	1094:1100	arg1	base					1167:1170	the hypocotyl base	1153:1170	the hypocotyl base	1153:1170	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	7	44	from	changes	1094:1100	arg1	content					1183:1189	mannan content	1176:1189	mannan content	1176:1189	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	7	44	from	changes	1094:1100	arg1	organization					1121:1132	cellulose fibre organization	1105:1132	cellulose fibre organization	1105:1132	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	4	45	theme	effect	443:448	arg1	advantage					426:434	advantage	426:434	advantage of the effect of brassinosteroids (BRs) on the two types of graviresponses in Arabidopsis thaliana	426:533	We took advantage of the effect of brassinosteroids (BRs) on the two types of graviresponses in Arabidopsis thaliana hypocotyls to disentangle functions of cell wall polymers during etiolated shoot growth.
33570567	5	46	theme	biosynthesis	816:827	arg1	brassinazole					777:788	brassinazole	777:788	brassinazole (BRZ)	777:794	The ability of etiolated Arabidopsis seedlings to grow upward was suppressed in the presence of 24-epibrassinolide (EBL) but enhanced in the presence of brassinazole (BRZ), an inhibitor of BR biosynthesis.
33570567	5	46	theme	biosynthesis	816:827	arg1	inhibitor					800:808	an inhibitor	797:808	an inhibitor of BR biosynthesis	797:827	The ability of etiolated Arabidopsis seedlings to grow upward was suppressed in the presence of 24-epibrassinolide (EBL) but enhanced in the presence of brassinazole (BRZ), an inhibitor of BR biosynthesis.
33570567	4	47	from	thaliana	526:533	arg1	types					487:491	the two types	479:491	the two types of graviresponses in Arabidopsis thaliana	479:533	We took advantage of the effect of brassinosteroids (BRs) on the two types of graviresponses in Arabidopsis thaliana hypocotyls to disentangle functions of cell wall polymers during etiolated shoot growth.
33570567	4	48	theme	Arabidopsis	514:524	arg1	thaliana					526:533	Arabidopsis thaliana	514:533	Arabidopsis thaliana	514:533	We took advantage of the effect of brassinosteroids (BRs) on the two types of graviresponses in Arabidopsis thaliana hypocotyls to disentangle functions of cell wall polymers during etiolated shoot growth.
33570567	4	49	from	effect	443:448	arg1	types					487:491	the two types	479:491	the two types of graviresponses in Arabidopsis thaliana	479:533	We took advantage of the effect of brassinosteroids (BRs) on the two types of graviresponses in Arabidopsis thaliana hypocotyls to disentangle functions of cell wall polymers during etiolated shoot growth.
33570567	9	50	theme	hemicelluloses	1473:1486	arg1	class					1464:1468	this class	1459:1468	this class of hemicelluloses	1459:1486	The negative effect of mannans on gravitropism is a new function for this class of hemicelluloses.
33570567	8	51	theme	hypocotyls	1286:1295	arg1	base					1278:1281	the base	1274:1281	the base of hypocotyls	1274:1295	Indeed, a longitudinal reorientation of cellulose fibres and growth inhibition at the base of hypocotyls supported their upright posture whereas the presence of mannans reduced gravitropic bending.
33570567	8	52	from	base	1278:1281	arg1	inhibition					1260:1269	growth inhibition	1253:1269	growth inhibition at the base of hypocotyls	1253:1295	Indeed, a longitudinal reorientation of cellulose fibres and growth inhibition at the base of hypocotyls supported their upright posture whereas the presence of mannans reduced gravitropic bending.
33570567	8	52	from	base	1278:1281	arg1	reorientation					1215:1227	a longitudinal reorientation	1200:1227	a longitudinal reorientation of cellulose fibres	1200:1247	Indeed, a longitudinal reorientation of cellulose fibres and growth inhibition at the base of hypocotyls supported their upright posture whereas the presence of mannans reduced gravitropic bending.
33570567	3	53	theme	organs	321:326	arg1	growth					304:309	the growth	300:309	the growth of aerial organs	300:326	These responses are essential for plants to direct the growth of aerial organs away from the soil surface after germination and to keep an upright posture above ground.
33570567	4	54	theme	brassinosteroids	453:468	arg1	effect					443:448	the effect	439:448	the effect of brassinosteroids (BRs) on the two types of graviresponses in Arabidopsis thaliana	439:533	We took advantage of the effect of brassinosteroids (BRs) on the two types of graviresponses in Arabidopsis thaliana hypocotyls to disentangle functions of cell wall polymers during etiolated shoot growth.
33570567	7	55	theme	growth	1021:1026	arg1	analyses					1028:1035	cellular growth analyses	1012:1035	cellular growth analyses	1012:1035	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	7	56	theme	EBL	1055:1057	arg1	treatments					1067:1076	the EBL and BRZ treatments	1051:1076	treatments	1067:1076	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	5	57	theme	24-epibrassinolide	720:737	arg1	presence					708:715	the presence	704:715	the presence of 24-epibrassinolide (EBL)	704:743	The ability of etiolated Arabidopsis seedlings to grow upward was suppressed in the presence of 24-epibrassinolide (EBL) but enhanced in the presence of brassinazole (BRZ), an inhibitor of BR biosynthesis.
33570567	2	58	theme	gravity	229:235	arg1	resistance					237:246	gravity resistance	229:246	gravity resistance	229:246	Plant shoots respond to gravity through negative gravitropism and gravity resistance.
33570567	7	59	theme	S4B	1000:1002	arg1	dye					1004:1006	the cellulose-specific pontamine S4B dye	967:1006	the cellulose-specific pontamine S4B dye	967:1006	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	4	60	from	advantage	426:434	arg1	types					487:491	the two types	479:491	the two types of graviresponses in Arabidopsis thaliana	479:533	We took advantage of the effect of brassinosteroids (BRs) on the two types of graviresponses in Arabidopsis thaliana hypocotyls to disentangle functions of cell wall polymers during etiolated shoot growth.
33570567	7	61	theme	dye	1004:1006	arg1	analyses					1028:1035	cellular growth analyses	1012:1035	cellular growth analyses	1012:1035	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	7	61	theme	dye	1004:1006	arg1	analyses					934:941	Cell wall biochemical analyses	912:941	Cell wall biochemical analyses	912:941	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	7	61	theme	dye	1004:1006	arg1	microscopy					953:962	confocal microscopy	944:962	confocal microscopy of the cellulose-specific pontamine S4B dye	944:1006	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	1	62	theme	constant	132:139	arg1	force					110:114	The force	106:114	The force of gravity	106:125	The force of gravity is a constant environmental factor.
33570567	1	62	theme	constant	132:139	arg1	factor					155:160	a constant environmental factor	130:160	a constant environmental factor	130:160	The force of gravity is a constant environmental factor.
33570567	4	63	theme	shoot	610:614	arg1	growth					616:621	etiolated shoot growth	600:621	etiolated shoot growth	600:621	We took advantage of the effect of brassinosteroids (BRs) on the two types of graviresponses in Arabidopsis thaliana hypocotyls to disentangle functions of cell wall polymers during etiolated shoot growth.
33570567	3	64	theme	aerial	314:319	arg1	organs					321:326	aerial organs	314:326	aerial organs	314:326	These responses are essential for plants to direct the growth of aerial organs away from the soil surface after germination and to keep an upright posture above ground.
33570567	7	65	theme	cellulose-specific	971:988	arg1	dye					1004:1006	the cellulose-specific pontamine S4B dye	967:1006	the cellulose-specific pontamine S4B dye	967:1006	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	1	66	theme	environmental	141:153	arg1	force					110:114	The force	106:114	The force of gravity	106:125	The force of gravity is a constant environmental factor.
33570567	1	66	theme	environmental	141:153	arg1	factor					155:160	a constant environmental factor	130:160	a constant environmental factor	130:160	The force of gravity is a constant environmental factor.
33570567	4	67	theme	etiolated	600:608	arg1	growth					616:621	etiolated shoot growth	600:621	etiolated shoot growth	600:621	We took advantage of the effect of brassinosteroids (BRs) on the two types of graviresponses in Arabidopsis thaliana hypocotyls to disentangle functions of cell wall polymers during etiolated shoot growth.
33570567	3	68	theme	soil	342:345	arg1	surface					347:353	the soil surface	338:353	the soil surface	338:353	These responses are essential for plants to direct the growth of aerial organs away from the soil surface after germination and to keep an upright posture above ground.
33570567	2	69	theme	negative	203:210	arg1	gravitropism					212:223	negative gravitropism	203:223	negative gravitropism	203:223	Plant shoots respond to gravity through negative gravitropism and gravity resistance.
33570567	7	70	theme	pontamine	990:998	arg1	dye					1004:1006	the cellulose-specific pontamine S4B dye	967:1006	the cellulose-specific pontamine S4B dye	967:1006	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	8	71	theme	upright	1313:1319	arg1	posture					1321:1327	their upright posture	1307:1327	their upright posture	1307:1327	Indeed, a longitudinal reorientation of cellulose fibres and growth inhibition at the base of hypocotyls supported their upright posture whereas the presence of mannans reduced gravitropic bending.
33570567	7	72	theme	hypocotyl	1157:1165	arg1	base					1167:1170	the hypocotyl base	1153:1170	the hypocotyl base	1153:1170	Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content.
33570567	8	73	theme	cellulose	1232:1240	arg1	fibres					1242:1247	cellulose fibres	1232:1247	cellulose fibres	1232:1247	Indeed, a longitudinal reorientation of cellulose fibres and growth inhibition at the base of hypocotyls supported their upright posture whereas the presence of mannans reduced gravitropic bending.
34354534	0	0	theme	Substrate	85:93	arg1	Specificities					95:107	Broad Substrate Specificities	79:107	Broad Substrate Specificities	79:107	Microbial α-L-Rhamnosidases of Glycosyl Hydrolase Families GH78 and GH106 Have Broad Substrate Specificities toward α-L-Rhamnosyl- and α-L-Mannosyl-Linkages.
34354534	0	1	contain	Have	74:77	arg1	α-L-Rhamnosidases					10:26	Microbial α-L-Rhamnosidases	0:26	Microbial α-L-Rhamnosidases of Glycosyl Hydrolase Families GH78 and GH106	0:72	Microbial α-L-Rhamnosidases of Glycosyl Hydrolase Families GH78 and GH106 Have Broad Substrate Specificities toward α-L-Rhamnosyl- and α-L-Mannosyl-Linkages.
34354534	0	1	contain	Have	74:77	arg2	Specificities					95:107	Broad Substrate Specificities	79:107	Broad Substrate Specificities	79:107	Microbial α-L-Rhamnosidases of Glycosyl Hydrolase Families GH78 and GH106 Have Broad Substrate Specificities toward α-L-Rhamnosyl- and α-L-Mannosyl-Linkages.
34354534	1	2	theme	L-rhamnose	269:278	arg1	residue					280:286	a terminal α-linked L-rhamnose residue	249:286	a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids	249:398	α-L-Rhamnosidases (α-L-Rha-ases, EC 3.2.1.40) are glycosyl hydrolases (GHs) that hydrolyze a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids.
34354534	0	3	theme	Broad	79:83	arg1	Specificities					95:107	Broad Substrate Specificities	79:107	Broad Substrate Specificities	79:107	Microbial α-L-Rhamnosidases of Glycosyl Hydrolase Families GH78 and GH106 Have Broad Substrate Specificities toward α-L-Rhamnosyl- and α-L-Mannosyl-Linkages.
34354534	8	4	theme	pH	1176:1177	arg1	dependence					1179:1188	pH dependence	1176:1188	pH dependence	1176:1188	The α-L-mannosidase activity of SpRhaM showed pH dependence, with highest activity observed at pH 7.0.
34354534	10	5	theme	polysaccharides	1418:1432	arg1	determination					1378:1390	structural determination	1367:1390	structural determination	1367:1390	Our findings will be useful in the identification and structural determination of α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries.
34354534	10	5	theme	polysaccharides	1418:1432	arg1	identification					1348:1361	identification	1348:1361	identification	1348:1361	Our findings will be useful in the identification and structural determination of α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries.
34354534	4	6	theme	microbial	617:625	arg1	α-L-Rha-ases					627:638	microbial α-L-Rha-ases	617:638	microbial α-L-Rha-ases	617:638	Here we have examined whether microbial α-L-Rha-ases possess α-L-mannosidase activity by synthesizing the substrate 4-nitrophenyl α-L-mannopyranoside.
34354534	5	7	theme	Escherichia	818:828	arg1	cells					835:839	Escherichia coli cells	818:839	Escherichia coli cells	818:839	Four α-L-Rha-ases from GH78 and GH106 families were expressed and purified from Escherichia coli cells.
34354534	7	8	theme	GH78	1111:1114	arg1	α-L-Rha-ases					1116:1127	three GH78 α-L-Rha-ases	1105:1127	three GH78 α-L-Rha-ases	1105:1127	SpRhaM, a GH106 family α-L-Rha-ase from Sphingomonas paucimobilis FP2001, was found to have relatively higher α-L-mannosidase activity as compared with three GH78 α-L-Rha-ases.
34354534	1	9	dep	α-L-Rhamnosidases	158:174	arg1	EC					191:192	EC 3.2.1.40	191:201	EC 3.2.1.40	191:201	α-L-Rhamnosidases (α-L-Rha-ases, EC 3.2.1.40) are glycosyl hydrolases (GHs) that hydrolyze a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids.
34354534	1	9	dep	α-L-Rhamnosidases	158:174	arg1	α-L-Rha-ases					177:188	α-L-Rha-ases	177:188	α-L-Rha-ases	177:188	α-L-Rhamnosidases (α-L-Rha-ases, EC 3.2.1.40) are glycosyl hydrolases (GHs) that hydrolyze a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids.
34354534	10	10	theme	α-L-mannose-containing	1395:1416	arg1	polysaccharides					1418:1432	α-L-mannose-containing polysaccharides	1395:1432	α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries	1395:1503	Our findings will be useful in the identification and structural determination of α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries.
34354534	4	11	theme	α-L-mannosidase	648:662	arg1	activity					664:671	α-L-mannosidase activity	648:671	α-L-mannosidase activity	648:671	Here we have examined whether microbial α-L-Rha-ases possess α-L-mannosidase activity by synthesizing the substrate 4-nitrophenyl α-L-mannopyranoside.
34354534	1	12	theme	natural	381:387	arg1	flavonoids					389:398	natural flavonoids	381:398	natural flavonoids	381:398	α-L-Rhamnosidases (α-L-Rha-ases, EC 3.2.1.40) are glycosyl hydrolases (GHs) that hydrolyze a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids.
34354534	6	13	theme	α-L-mannosyl-hydrolyzing	918:941	arg1	activity					943:950	weak α-L-mannosyl-hydrolyzing activity	913:950	weak α-L-mannosyl-hydrolyzing activity	913:950	All four enzymes exhibited both α-L-rhamnosyl-hydrolyzing activity and weak α-L-mannosyl-hydrolyzing activity.
34354534	6	14	theme	weak	913:916	arg1	activity					943:950	weak α-L-mannosyl-hydrolyzing activity	913:950	weak α-L-mannosyl-hydrolyzing activity	913:950	All four enzymes exhibited both α-L-rhamnosyl-hydrolyzing activity and weak α-L-mannosyl-hydrolyzing activity.
34354534	3	15	theme	sugar	568:572	arg1	α-L-mannose					574:584	the rare sugar α-L-mannose	559:584	the rare sugar α-L-mannose	559:584	α-L-rhamnose (6-deoxy-L-mannose) is structurally similar to the rare sugar α-L-mannose.
34354534	1	16	gly	glycosylated	354:365	arg1	proteins					367:374	glycosylated proteins	354:374	glycosylated proteins	354:374	α-L-Rhamnosidases (α-L-Rha-ases, EC 3.2.1.40) are glycosyl hydrolases (GHs) that hydrolyze a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids.
34354534	8	17	theme	highest	1196:1202	arg1	activity					1204:1211	highest activity	1196:1211	highest activity observed at pH 7.0	1196:1230	The α-L-mannosidase activity of SpRhaM showed pH dependence, with highest activity observed at pH 7.0.
34354534	0	18	theme	Microbial	0:8	arg1	α-L-Rhamnosidases					10:26	Microbial α-L-Rhamnosidases	0:26	Microbial α-L-Rhamnosidases of Glycosyl Hydrolase Families GH78 and GH106	0:72	Microbial α-L-Rhamnosidases of Glycosyl Hydrolase Families GH78 and GH106 Have Broad Substrate Specificities toward α-L-Rhamnosyl- and α-L-Mannosyl-Linkages.
34354534	7	19	contain	have	1040:1043	arg1	α-L-Rha-ase					976:986	a GH106 family α-L-Rha-ase	961:986	a GH106 family α-L-Rha-ase from Sphingomonas paucimobilis FP2001	961:1024	SpRhaM, a GH106 family α-L-Rha-ase from Sphingomonas paucimobilis FP2001, was found to have relatively higher α-L-mannosidase activity as compared with three GH78 α-L-Rha-ases.
34354534	7	19	contain	have	1040:1043	arg1	SpRhaM					953:958	SpRhaM	953:958	SpRhaM	953:958	SpRhaM, a GH106 family α-L-Rha-ase from Sphingomonas paucimobilis FP2001, was found to have relatively higher α-L-mannosidase activity as compared with three GH78 α-L-Rha-ases.
34354534	7	19	contain	have	1040:1043	arg2	activity					1079:1086	relatively higher α-L-mannosidase activity	1045:1086	relatively higher α-L-mannosidase activity	1045:1086	SpRhaM, a GH106 family α-L-Rha-ase from Sphingomonas paucimobilis FP2001, was found to have relatively higher α-L-mannosidase activity as compared with three GH78 α-L-Rha-ases.
34354534	2	20	theme	biotechnological	468:483	arg1	applications					485:496	various biotechnological applications	460:496	various biotechnological applications	460:496	As a result, they are considered catalysts of interest for various biotechnological applications.
34354534	1	21	link	α-linked	260:267	arg1	residue					280:286	a terminal α-linked L-rhamnose residue	249:286	a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids	249:398	α-L-Rhamnosidases (α-L-Rha-ases, EC 3.2.1.40) are glycosyl hydrolases (GHs) that hydrolyze a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids.
34354534	10	22	from	useful	1334:1339	arg1	determination					1378:1390	structural determination	1367:1390	structural determination	1367:1390	Our findings will be useful in the identification and structural determination of α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries.
34354534	10	22	from	useful	1334:1339	arg1	identification					1348:1361	identification	1348:1361	identification	1348:1361	Our findings will be useful in the identification and structural determination of α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries.
34354534	1	23	theme	glycosyl	208:215	arg1	α-L-Rhamnosidases					158:174	α-L-Rhamnosidases	158:174	α-L-Rhamnosidases (α-L-Rha-ases, EC 3.2.1.40)	158:202	α-L-Rhamnosidases (α-L-Rha-ases, EC 3.2.1.40) are glycosyl hydrolases (GHs) that hydrolyze a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids.
34354534	1	23	theme	glycosyl	208:215	arg1	GHs					229:231	GHs	229:231	GHs	229:231	α-L-Rhamnosidases (α-L-Rha-ases, EC 3.2.1.40) are glycosyl hydrolases (GHs) that hydrolyze a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids.
34354534	1	23	theme	glycosyl	208:215	arg1	hydrolases					217:226	glycosyl hydrolases	208:226	glycosyl hydrolases (GHs) that hydrolyze a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids	208:398	α-L-Rhamnosidases (α-L-Rha-ases, EC 3.2.1.40) are glycosyl hydrolases (GHs) that hydrolyze a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids.
34354534	2	24	theme	various	460:466	arg1	applications					485:496	various biotechnological applications	460:496	various biotechnological applications	460:496	As a result, they are considered catalysts of interest for various biotechnological applications.
34354534	1	25	theme	wide	295:298	arg1	spectrum					300:307	a wide spectrum	293:307	a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids	293:398	α-L-Rhamnosidases (α-L-Rha-ases, EC 3.2.1.40) are glycosyl hydrolases (GHs) that hydrolyze a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids.
34354534	1	26	from	spectrum	300:307	arg1	residue					280:286	a terminal α-linked L-rhamnose residue	249:286	a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids	249:398	α-L-Rhamnosidases (α-L-Rha-ases, EC 3.2.1.40) are glycosyl hydrolases (GHs) that hydrolyze a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids.
34354534	6	27	theme	α-L-rhamnosyl-hydrolyzing	874:898	arg1	activity					900:907	α-L-rhamnosyl-hydrolyzing activity	874:907	α-L-rhamnosyl-hydrolyzing activity	874:907	All four enzymes exhibited both α-L-rhamnosyl-hydrolyzing activity and weak α-L-mannosyl-hydrolyzing activity.
34354534	10	28	theme	food	1489:1492	arg1	industries					1494:1503	the pharmaceutical and food industries	1466:1503	the pharmaceutical and food industries	1466:1503	Our findings will be useful in the identification and structural determination of α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries.
34354534	0	29	theme	Glycosyl	31:38	arg1	Families					50:57	Glycosyl Hydrolase Families GH78 and GH106	31:72	Glycosyl Hydrolase Families GH78 and GH106	31:72	Microbial α-L-Rhamnosidases of Glycosyl Hydrolase Families GH78 and GH106 Have Broad Substrate Specificities toward α-L-Rhamnosyl- and α-L-Mannosyl-Linkages.
34354534	0	29	theme	Glycosyl	31:38	arg1	GH106					68:72	GH106	68:72	GH106	68:72	Microbial α-L-Rhamnosidases of Glycosyl Hydrolase Families GH78 and GH106 Have Broad Substrate Specificities toward α-L-Rhamnosyl- and α-L-Mannosyl-Linkages.
34354534	0	29	theme	Glycosyl	31:38	arg1	GH78					59:62	GH78	59:62	GH78	59:62	Microbial α-L-Rhamnosidases of Glycosyl Hydrolase Families GH78 and GH106 Have Broad Substrate Specificities toward α-L-Rhamnosyl- and α-L-Mannosyl-Linkages.
34354534	7	30	theme	higher	1056:1061	arg1	activity					1079:1086	relatively higher α-L-mannosidase activity	1045:1086	relatively higher α-L-mannosidase activity	1045:1086	SpRhaM, a GH106 family α-L-Rha-ase from Sphingomonas paucimobilis FP2001, was found to have relatively higher α-L-mannosidase activity as compared with three GH78 α-L-Rha-ases.
34354534	7	31	theme	α-L-mannosidase	1063:1077	arg1	activity					1079:1086	relatively higher α-L-mannosidase activity	1045:1086	relatively higher α-L-mannosidase activity	1045:1086	SpRhaM, a GH106 family α-L-Rha-ase from Sphingomonas paucimobilis FP2001, was found to have relatively higher α-L-mannosidase activity as compared with three GH78 α-L-Rha-ases.
34354534	7	32	theme	GH106	963:967	arg1	α-L-Rha-ase					976:986	a GH106 family α-L-Rha-ase	961:986	a GH106 family α-L-Rha-ase from Sphingomonas paucimobilis FP2001	961:1024	SpRhaM, a GH106 family α-L-Rha-ase from Sphingomonas paucimobilis FP2001, was found to have relatively higher α-L-mannosidase activity as compared with three GH78 α-L-Rha-ases.
34354534	7	32	theme	GH106	963:967	arg1	SpRhaM					953:958	SpRhaM	953:958	SpRhaM	953:958	SpRhaM, a GH106 family α-L-Rha-ase from Sphingomonas paucimobilis FP2001, was found to have relatively higher α-L-mannosidase activity as compared with three GH78 α-L-Rha-ases.
34354534	4	33	theme	4-nitrophenyl	703:715	arg1	α-L-mannopyranoside					717:735	the substrate 4-nitrophenyl α-L-mannopyranoside	689:735	the substrate 4-nitrophenyl α-L-mannopyranoside	689:735	Here we have examined whether microbial α-L-Rha-ases possess α-L-mannosidase activity by synthesizing the substrate 4-nitrophenyl α-L-mannopyranoside.
34354534	2	34	theme	interest	447:454	arg1	they					414:417	they	414:417	they	414:417	As a result, they are considered catalysts of interest for various biotechnological applications.
34354534	2	34	theme	interest	447:454	arg1	catalysts					434:442	catalysts	434:442	catalysts of interest for various biotechnological applications	434:496	As a result, they are considered catalysts of interest for various biotechnological applications.
34354534	2	34	theme	interest	447:454	arg1	result					406:411	a result	404:411	a result	404:411	As a result, they are considered catalysts of interest for various biotechnological applications.
34354534	10	35	theme	pharmaceutical	1470:1483	arg1	industries					1494:1503	the pharmaceutical and food industries	1466:1503	the pharmaceutical and food industries	1466:1503	Our findings will be useful in the identification and structural determination of α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries.
34354534	4	36	theme	substrate	693:701	arg1	α-L-mannopyranoside					717:735	the substrate 4-nitrophenyl α-L-mannopyranoside	689:735	the substrate 4-nitrophenyl α-L-mannopyranoside	689:735	Here we have examined whether microbial α-L-Rha-ases possess α-L-mannosidase activity by synthesizing the substrate 4-nitrophenyl α-L-mannopyranoside.
34354534	0	37	theme	Families	50:57	arg1	α-L-Rhamnosidases					10:26	Microbial α-L-Rhamnosidases	0:26	Microbial α-L-Rhamnosidases of Glycosyl Hydrolase Families GH78 and GH106	0:72	Microbial α-L-Rhamnosidases of Glycosyl Hydrolase Families GH78 and GH106 Have Broad Substrate Specificities toward α-L-Rhamnosyl- and α-L-Mannosyl-Linkages.
34354534	1	38	theme	substrates	312:321	arg1	spectrum					300:307	a wide spectrum	293:307	a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids	293:398	α-L-Rhamnosidases (α-L-Rha-ases, EC 3.2.1.40) are glycosyl hydrolases (GHs) that hydrolyze a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids.
34354534	4	39	contain	possess	640:646	arg1	α-L-Rha-ases					627:638	microbial α-L-Rha-ases	617:638	microbial α-L-Rha-ases	617:638	Here we have examined whether microbial α-L-Rha-ases possess α-L-mannosidase activity by synthesizing the substrate 4-nitrophenyl α-L-mannopyranoside.
34354534	4	39	contain	possess	640:646	arg2	activity					664:671	α-L-mannosidase activity	648:671	α-L-mannosidase activity	648:671	Here we have examined whether microbial α-L-Rha-ases possess α-L-mannosidase activity by synthesizing the substrate 4-nitrophenyl α-L-mannopyranoside.
34354534	8	40	located	observed	1213:1220	arg1	pH					1225:1226	pH 7.0	1225:1230	pH 7.0	1225:1230	The α-L-mannosidase activity of SpRhaM showed pH dependence, with highest activity observed at pH 7.0.
34354534	8	40	located	observed	1213:1220	arg2	activity					1204:1211	highest activity	1196:1211	highest activity observed at pH 7.0	1196:1230	The α-L-mannosidase activity of SpRhaM showed pH dependence, with highest activity observed at pH 7.0.
34354534	0	41	theme	Hydrolase	40:48	arg1	Families					50:57	Glycosyl Hydrolase Families GH78 and GH106	31:72	Glycosyl Hydrolase Families GH78 and GH106	31:72	Microbial α-L-Rhamnosidases of Glycosyl Hydrolase Families GH78 and GH106 Have Broad Substrate Specificities toward α-L-Rhamnosyl- and α-L-Mannosyl-Linkages.
34354534	0	41	theme	Hydrolase	40:48	arg1	GH106					68:72	GH106	68:72	GH106	68:72	Microbial α-L-Rhamnosidases of Glycosyl Hydrolase Families GH78 and GH106 Have Broad Substrate Specificities toward α-L-Rhamnosyl- and α-L-Mannosyl-Linkages.
34354534	0	41	theme	Hydrolase	40:48	arg1	GH78					59:62	GH78	59:62	GH78	59:62	Microbial α-L-Rhamnosidases of Glycosyl Hydrolase Families GH78 and GH106 Have Broad Substrate Specificities toward α-L-Rhamnosyl- and α-L-Mannosyl-Linkages.
34354534	8	42	theme	α-L-mannosidase	1134:1148	arg1	activity					1150:1157	The α-L-mannosidase activity	1130:1157	The α-L-mannosidase activity of SpRhaM	1130:1167	The α-L-mannosidase activity of SpRhaM showed pH dependence, with highest activity observed at pH 7.0.
34354534	10	43	dep	identification	1348:1361	arg1	the					1344:1346	the	1344:1346	the	1344:1346	Our findings will be useful in the identification and structural determination of α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries.
34354534	10	44	from	sources	1447:1453	arg1	determination					1378:1390	structural determination	1367:1390	structural determination	1367:1390	Our findings will be useful in the identification and structural determination of α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries.
34354534	10	44	from	sources	1447:1453	arg1	polysaccharides					1418:1432	α-L-mannose-containing polysaccharides	1395:1432	α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries	1395:1503	Our findings will be useful in the identification and structural determination of α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries.
34354534	10	44	from	sources	1447:1453	arg1	identification					1348:1361	identification	1348:1361	identification	1348:1361	Our findings will be useful in the identification and structural determination of α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries.
34354534	9	45	contain	have	1282:1285	arg1	α-L-Rha-ases					1264:1275	α-L-Rha-ases	1264:1275	α-L-Rha-ases	1264:1275	In summary, we have shown that α-L-Rha-ases also have α-L-mannosidase activity.
34354534	9	45	contain	have	1282:1285	arg2	activity					1303:1310	α-L-mannosidase activity	1287:1310	α-L-mannosidase activity	1287:1310	In summary, we have shown that α-L-Rha-ases also have α-L-mannosidase activity.
34354534	5	46	from	GH78	761:764	arg1	α-L-Rha-ases					743:754	Four α-L-Rha-ases	738:754	Four α-L-Rha-ases from GH78 and GH106 families	738:783	Four α-L-Rha-ases from GH78 and GH106 families were expressed and purified from Escherichia coli cells.
34354534	0	47	dep	Families	50:57	arg1	Families					50:57	Glycosyl Hydrolase Families GH78 and GH106	31:72	Glycosyl Hydrolase Families GH78 and GH106	31:72	Microbial α-L-Rhamnosidases of Glycosyl Hydrolase Families GH78 and GH106 Have Broad Substrate Specificities toward α-L-Rhamnosyl- and α-L-Mannosyl-Linkages.
34354534	0	47	dep	Families	50:57	arg1	GH106					68:72	GH106	68:72	GH106	68:72	Microbial α-L-Rhamnosidases of Glycosyl Hydrolase Families GH78 and GH106 Have Broad Substrate Specificities toward α-L-Rhamnosyl- and α-L-Mannosyl-Linkages.
34354534	0	47	dep	Families	50:57	arg1	GH78					59:62	GH78	59:62	GH78	59:62	Microbial α-L-Rhamnosidases of Glycosyl Hydrolase Families GH78 and GH106 Have Broad Substrate Specificities toward α-L-Rhamnosyl- and α-L-Mannosyl-Linkages.
34354534	10	48	from	identification	1348:1361	arg1	sources					1447:1453	natural sources	1439:1453	natural sources for use in the pharmaceutical and food industries	1439:1503	Our findings will be useful in the identification and structural determination of α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries.
34354534	10	48	from	identification	1348:1361	arg1	useful					1334:1339	useful	1334:1339	useful	1334:1339	Our findings will be useful in the identification and structural determination of α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries.
34354534	5	49	theme	GH106	770:774	arg1	families					776:783	GH106 families	770:783	GH106 families	770:783	Four α-L-Rha-ases from GH78 and GH106 families were expressed and purified from Escherichia coli cells.
34354534	10	50	from	use	1459:1461	arg1	industries					1494:1503	the pharmaceutical and food industries	1466:1503	the pharmaceutical and food industries	1466:1503	Our findings will be useful in the identification and structural determination of α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries.
34354534	8	51	theme	SpRhaM	1162:1167	arg1	activity					1150:1157	The α-L-mannosidase activity	1130:1157	The α-L-mannosidase activity of SpRhaM	1130:1167	The α-L-mannosidase activity of SpRhaM showed pH dependence, with highest activity observed at pH 7.0.
34354534	5	52	dep	Escherichia	818:828	arg1	coli					830:833	coli	830:833	coli	830:833	Four α-L-Rha-ases from GH78 and GH106 families were expressed and purified from Escherichia coli cells.
34354534	7	53	from	paucimobilis	1006:1017	arg1	α-L-Rha-ase					976:986	a GH106 family α-L-Rha-ase	961:986	a GH106 family α-L-Rha-ase from Sphingomonas paucimobilis FP2001	961:1024	SpRhaM, a GH106 family α-L-Rha-ase from Sphingomonas paucimobilis FP2001, was found to have relatively higher α-L-mannosidase activity as compared with three GH78 α-L-Rha-ases.
34354534	7	53	from	paucimobilis	1006:1017	arg1	SpRhaM					953:958	SpRhaM	953:958	SpRhaM	953:958	SpRhaM, a GH106 family α-L-Rha-ase from Sphingomonas paucimobilis FP2001, was found to have relatively higher α-L-mannosidase activity as compared with three GH78 α-L-Rha-ases.
34354534	5	54	from	families	776:783	arg1	α-L-Rha-ases					743:754	Four α-L-Rha-ases	738:754	Four α-L-Rha-ases from GH78 and GH106 families	738:783	Four α-L-Rha-ases from GH78 and GH106 families were expressed and purified from Escherichia coli cells.
34354534	10	55	from	determination	1378:1390	arg1	sources					1447:1453	natural sources	1439:1453	natural sources for use in the pharmaceutical and food industries	1439:1503	Our findings will be useful in the identification and structural determination of α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries.
34354534	10	55	from	determination	1378:1390	arg1	useful					1334:1339	useful	1334:1339	useful	1334:1339	Our findings will be useful in the identification and structural determination of α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries.
34354534	7	56	theme	Sphingomonas	993:1004	arg1	paucimobilis					1006:1017	Sphingomonas paucimobilis FP2001	993:1024	Sphingomonas paucimobilis FP2001	993:1024	SpRhaM, a GH106 family α-L-Rha-ase from Sphingomonas paucimobilis FP2001, was found to have relatively higher α-L-mannosidase activity as compared with three GH78 α-L-Rha-ases.
34354534	10	57	theme	structural	1367:1376	arg1	determination					1378:1390	structural determination	1367:1390	structural determination	1367:1390	Our findings will be useful in the identification and structural determination of α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries.
34354534	7	58	theme	family	969:974	arg1	α-L-Rha-ase					976:986	a GH106 family α-L-Rha-ase	961:986	a GH106 family α-L-Rha-ase from Sphingomonas paucimobilis FP2001	961:1024	SpRhaM, a GH106 family α-L-Rha-ase from Sphingomonas paucimobilis FP2001, was found to have relatively higher α-L-mannosidase activity as compared with three GH78 α-L-Rha-ases.
34354534	7	58	theme	family	969:974	arg1	SpRhaM					953:958	SpRhaM	953:958	SpRhaM	953:958	SpRhaM, a GH106 family α-L-Rha-ase from Sphingomonas paucimobilis FP2001, was found to have relatively higher α-L-mannosidase activity as compared with three GH78 α-L-Rha-ases.
34354534	9	59	theme	α-L-mannosidase	1287:1301	arg1	activity					1303:1310	α-L-mannosidase activity	1287:1310	α-L-mannosidase activity	1287:1310	In summary, we have shown that α-L-Rha-ases also have α-L-mannosidase activity.
34354534	1	60	theme	terminal	251:258	arg1	residue					280:286	a terminal α-linked L-rhamnose residue	249:286	a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids	249:398	α-L-Rhamnosidases (α-L-Rha-ases, EC 3.2.1.40) are glycosyl hydrolases (GHs) that hydrolyze a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids.
34354534	1	61	theme	glycosylated	354:365	arg1	proteins					367:374	glycosylated proteins	354:374	glycosylated proteins	354:374	α-L-Rhamnosidases (α-L-Rha-ases, EC 3.2.1.40) are glycosyl hydrolases (GHs) that hydrolyze a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids.
34354534	3	62	theme	rare	563:566	arg1	α-L-mannose					574:584	the rare sugar α-L-mannose	559:584	the rare sugar α-L-mannose	559:584	α-L-rhamnose (6-deoxy-L-mannose) is structurally similar to the rare sugar α-L-mannose.
34354534	10	63	theme	natural	1439:1445	arg1	sources					1447:1453	natural sources	1439:1453	natural sources for use in the pharmaceutical and food industries	1439:1503	Our findings will be useful in the identification and structural determination of α-L-mannose-containing polysaccharides from natural sources for use in the pharmaceutical and food industries.
34354534	1	64	theme	α-linked	260:267	arg1	residue					280:286	a terminal α-linked L-rhamnose residue	249:286	a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids	249:398	α-L-Rhamnosidases (α-L-Rha-ases, EC 3.2.1.40) are glycosyl hydrolases (GHs) that hydrolyze a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids.
33448225	4	0	theme	mAb	775:777	arg1	lower					839:843	lower	839:843	lower	839:843	The affinity of the hIgG2 mAb for a positively charged copolymer-grafted sorbent was much lower than that found for the negatively charged copolymer-grafted sorbents at both 20 and 50 °C due to electrostatic repulsive effects.
33448225	4	0	theme	mAb	775:777	arg1	affinity					753:760	The affinity	749:760	The affinity of the hIgG2 mAb for a positively charged copolymer-grafted sorbent	749:828	The affinity of the hIgG2 mAb for a positively charged copolymer-grafted sorbent was much lower than that found for the negatively charged copolymer-grafted sorbents at both 20 and 50 °C due to electrostatic repulsive effects.
33448225	4	1	theme	repulsive	957:965	arg1	effects					967:973	electrostatic repulsive effects	943:973	electrostatic repulsive effects	943:973	The affinity of the hIgG2 mAb for a positively charged copolymer-grafted sorbent was much lower than that found for the negatively charged copolymer-grafted sorbents at both 20 and 50 °C due to electrostatic repulsive effects.
33448225	1	2	theme	antibody	172:179	arg1	behavior					137:144	The batch adsorption behavior	116:144	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers	116:315	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers is described.
33448225	1	3	theme	Flow	253:256	arg1	sorbents					258:265	thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents	198:265	thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers	198:315	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers is described.
33448225	0	4	theme	Fast	96:99	arg1	Flow					101:104	Thermoresponsive Copolymer-Grafted Sepharose Fast Flow	51:104	Thermoresponsive Copolymer-Grafted Sepharose Fast Flow	51:104	Adsorption of a Humanized Monoclonal Antibody onto Thermoresponsive Copolymer-Grafted Sepharose Fast Flow Sorbents.
33448225	4	5	theme	electrostatic	943:955	arg1	effects					967:973	electrostatic repulsive effects	943:973	electrostatic repulsive effects	943:973	The affinity of the hIgG2 mAb for a positively charged copolymer-grafted sorbent was much lower than that found for the negatively charged copolymer-grafted sorbents at both 20 and 50 °C due to electrostatic repulsive effects.
33448225	3	6	theme	charged	560:566	arg1	sorbents					587:594	the negatively charged copolymer-modified sorbents	545:594	the negatively charged copolymer-modified sorbents	545:594	The adsorption capacity of the negatively charged copolymer-modified sorbents increased with an increase in the applied environmental temperature due to increased protein-sorbent surface hydrophobic and electrostatic interactions.
33448225	5	7	theme	copolymer	1052:1060	arg1	functionalities					1021:1035	the molecular functionalities	1007:1035	the molecular functionalities of the grafted copolymer	1007:1060	This study has documented that the molecular functionalities of the grafted copolymer can significantly affect the adsorption behavior of this humanized mAb at both 20 and 50 °C with the isothermal dependencies revealing subtle effects due to copolymer composition.
33448225	4	8	theme	copolymer-grafted	804:820	arg1	sorbent					822:828	a positively charged copolymer-grafted sorbent	783:828	a positively charged copolymer-grafted sorbent	783:828	The affinity of the hIgG2 mAb for a positively charged copolymer-grafted sorbent was much lower than that found for the negatively charged copolymer-grafted sorbents at both 20 and 50 °C due to electrostatic repulsive effects.
33448225	1	9	theme	different	272:280	arg1	compositions					282:293	different compositions	272:293	different compositions of grafted copolymers	272:315	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers is described.
33448225	4	10	theme	charged	796:802	arg1	sorbent					822:828	a positively charged copolymer-grafted sorbent	783:828	a positively charged copolymer-grafted sorbent	783:828	The affinity of the hIgG2 mAb for a positively charged copolymer-grafted sorbent was much lower than that found for the negatively charged copolymer-grafted sorbents at both 20 and 50 °C due to electrostatic repulsive effects.
33448225	5	11	with	°C	1151:1152	arg1	dependencies					1174:1185	the isothermal dependencies	1159:1185	the isothermal dependencies revealing subtle effects due to copolymer composition	1159:1239	This study has documented that the molecular functionalities of the grafted copolymer can significantly affect the adsorption behavior of this humanized mAb at both 20 and 50 °C with the isothermal dependencies revealing subtle effects due to copolymer composition.
33448225	3	12	theme	sorbents	587:594	arg1	capacity					533:540	The adsorption capacity	518:540	The adsorption capacity of the negatively charged copolymer-modified sorbents	518:594	The adsorption capacity of the negatively charged copolymer-modified sorbents increased with an increase in the applied environmental temperature due to increased protein-sorbent surface hydrophobic and electrostatic interactions.
33448225	5	13	theme	molecular	1011:1019	arg1	functionalities					1021:1035	the molecular functionalities	1007:1035	the molecular functionalities of the grafted copolymer	1007:1060	This study has documented that the molecular functionalities of the grafted copolymer can significantly affect the adsorption behavior of this humanized mAb at both 20 and 50 °C with the isothermal dependencies revealing subtle effects due to copolymer composition.
33448225	4	14	from	°C	933:934	arg1	sorbents					906:913	the negatively charged copolymer-grafted sorbents	865:913	the negatively charged copolymer-grafted sorbents at both 20 and 50 °C due to electrostatic repulsive effects	865:973	The affinity of the hIgG2 mAb for a positively charged copolymer-grafted sorbent was much lower than that found for the negatively charged copolymer-grafted sorbents at both 20 and 50 °C due to electrostatic repulsive effects.
33448225	5	15	theme	copolymer	1219:1227	arg1	composition					1229:1239	copolymer composition	1219:1239	copolymer composition	1219:1239	This study has documented that the molecular functionalities of the grafted copolymer can significantly affect the adsorption behavior of this humanized mAb at both 20 and 50 °C with the isothermal dependencies revealing subtle effects due to copolymer composition.
33448225	3	16	theme	copolymer-modified	568:585	arg1	sorbents					587:594	the negatively charged copolymer-modified sorbents	545:594	the negatively charged copolymer-modified sorbents	545:594	The adsorption capacity of the negatively charged copolymer-modified sorbents increased with an increase in the applied environmental temperature due to increased protein-sorbent surface hydrophobic and electrostatic interactions.
33448225	5	17	theme	subtle	1197:1202	arg1	effects					1204:1210	subtle effects	1197:1210	subtle effects	1197:1210	This study has documented that the molecular functionalities of the grafted copolymer can significantly affect the adsorption behavior of this humanized mAb at both 20 and 50 °C with the isothermal dependencies revealing subtle effects due to copolymer composition.
33448225	2	18	theme	copolymer-modified	396:413	arg1	sorbents					415:422	negatively charged copolymer-modified sorbents	377:422	negatively charged copolymer-modified sorbents	377:422	At high protein loadings, the adsorption with negatively charged copolymer-modified sorbents exhibited S-shaped isotherms in most cases, indicative of unrestricted multilayer adsorption.
33448225	2	19	theme	indicative	468:477	arg1	isotherms					443:451	S-shaped isotherms	434:451	S-shaped isotherms	434:451	At high protein loadings, the adsorption with negatively charged copolymer-modified sorbents exhibited S-shaped isotherms in most cases, indicative of unrestricted multilayer adsorption.
33448225	1	20	with	behavior	137:144	arg1	sorbents					258:265	thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents	198:265	thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers	198:315	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers is described.
33448225	3	21	theme	applied	630:636	arg1	temperature					652:662	the applied environmental temperature	626:662	the applied environmental temperature	626:662	The adsorption capacity of the negatively charged copolymer-modified sorbents increased with an increase in the applied environmental temperature due to increased protein-sorbent surface hydrophobic and electrostatic interactions.
33448225	2	22	theme	charged	388:394	arg1	sorbents					415:422	negatively charged copolymer-modified sorbents	377:422	negatively charged copolymer-modified sorbents	377:422	At high protein loadings, the adsorption with negatively charged copolymer-modified sorbents exhibited S-shaped isotherms in most cases, indicative of unrestricted multilayer adsorption.
33448225	0	23	theme	Humanized	16:24	arg1	Antibody					37:44	a Humanized Monoclonal Antibody	14:44	a Humanized Monoclonal Antibody	14:44	Adsorption of a Humanized Monoclonal Antibody onto Thermoresponsive Copolymer-Grafted Sepharose Fast Flow Sorbents.
33448225	5	24	theme	grafted	1044:1050	arg1	copolymer					1052:1060	the grafted copolymer	1040:1060	the grafted copolymer	1040:1060	This study has documented that the molecular functionalities of the grafted copolymer can significantly affect the adsorption behavior of this humanized mAb at both 20 and 50 °C with the isothermal dependencies revealing subtle effects due to copolymer composition.
33448225	1	25	theme	thermoresponsive	198:213	arg1	sorbents					258:265	thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents	198:265	thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers	198:315	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers is described.
33448225	1	26	theme	grafted	298:304	arg1	copolymers					306:315	grafted copolymers	298:315	grafted copolymers	298:315	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers is described.
33448225	1	27	theme	batch	120:124	arg1	behavior					137:144	The batch adsorption behavior	116:144	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers	116:315	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers is described.
33448225	1	28	theme	polymer	215:221	arg1	sorbents					258:265	thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents	198:265	thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers	198:315	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers is described.
33448225	0	29	theme	Antibody	37:44	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption of a Humanized Monoclonal Antibody onto Thermoresponsive Copolymer-Grafted Sepharose Fast Flow	0:104	Adsorption of a Humanized Monoclonal Antibody onto Thermoresponsive Copolymer-Grafted Sepharose Fast Flow Sorbents.
33448225	1	30	theme	copolymers	306:315	arg1	compositions					282:293	different compositions	272:293	different compositions of grafted copolymers	272:315	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers is described.
33448225	5	31	theme	humanized	1119:1127	arg1	mAb					1129:1131	this humanized mAb	1114:1131	this humanized mAb	1114:1131	This study has documented that the molecular functionalities of the grafted copolymer can significantly affect the adsorption behavior of this humanized mAb at both 20 and 50 °C with the isothermal dependencies revealing subtle effects due to copolymer composition.
33448225	0	32	theme	Monoclonal	26:35	arg1	Antibody					37:44	a Humanized Monoclonal Antibody	14:44	a Humanized Monoclonal Antibody	14:44	Adsorption of a Humanized Monoclonal Antibody onto Thermoresponsive Copolymer-Grafted Sepharose Fast Flow Sorbents.
33448225	2	33	theme	adsorption	506:515	arg1	indicative					468:477	indicative	468:477	indicative	468:477	At high protein loadings, the adsorption with negatively charged copolymer-modified sorbents exhibited S-shaped isotherms in most cases, indicative of unrestricted multilayer adsorption.
33448225	5	34	theme	isothermal	1163:1172	arg1	dependencies					1174:1185	the isothermal dependencies	1159:1185	the isothermal dependencies revealing subtle effects due to copolymer composition	1159:1239	This study has documented that the molecular functionalities of the grafted copolymer can significantly affect the adsorption behavior of this humanized mAb at both 20 and 50 °C with the isothermal dependencies revealing subtle effects due to copolymer composition.
33448225	5	35	theme	mAb	1129:1131	arg1	behavior					1102:1109	the adsorption behavior	1087:1109	the adsorption behavior of this humanized mAb	1087:1131	This study has documented that the molecular functionalities of the grafted copolymer can significantly affect the adsorption behavior of this humanized mAb at both 20 and 50 °C with the isothermal dependencies revealing subtle effects due to copolymer composition.
33448225	1	36	theme	TRP	224:226	arg1	sorbents					258:265	thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents	198:265	thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers	198:315	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers is described.
33448225	2	37	theme	multilayer	495:504	arg1	adsorption					506:515	unrestricted multilayer adsorption	482:515	unrestricted multilayer adsorption	482:515	At high protein loadings, the adsorption with negatively charged copolymer-modified sorbents exhibited S-shaped isotherms in most cases, indicative of unrestricted multilayer adsorption.
33448225	3	38	theme	electrostatic	721:733	arg1	interactions					735:746	protein-sorbent surface hydrophobic and electrostatic interactions	681:746	increased protein-sorbent surface hydrophobic and electrostatic interactions	671:746	The adsorption capacity of the negatively charged copolymer-modified sorbents increased with an increase in the applied environmental temperature due to increased protein-sorbent surface hydrophobic and electrostatic interactions.
33448225	3	39	theme	environmental	638:650	arg1	temperature					652:662	the applied environmental temperature	626:662	the applied environmental temperature	626:662	The adsorption capacity of the negatively charged copolymer-modified sorbents increased with an increase in the applied environmental temperature due to increased protein-sorbent surface hydrophobic and electrostatic interactions.
33448225	4	40	theme	due	936:938	arg1	°C					933:934	both 20 and 50 °C	918:934	both 20 and 50 °C due to electrostatic repulsive effects	918:973	The affinity of the hIgG2 mAb for a positively charged copolymer-grafted sorbent was much lower than that found for the negatively charged copolymer-grafted sorbents at both 20 and 50 °C due to electrostatic repulsive effects.
33448225	1	41	theme	adsorption	126:135	arg1	behavior					137:144	The batch adsorption behavior	116:144	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers	116:315	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers is described.
33448225	2	42	theme	unrestricted	482:493	arg1	adsorption					506:515	unrestricted multilayer adsorption	482:515	unrestricted multilayer adsorption	482:515	At high protein loadings, the adsorption with negatively charged copolymer-modified sorbents exhibited S-shaped isotherms in most cases, indicative of unrestricted multilayer adsorption.
33448225	2	43	with	adsorption	361:370	arg1	sorbents					415:422	negatively charged copolymer-modified sorbents	377:422	negatively charged copolymer-modified sorbents	377:422	At high protein loadings, the adsorption with negatively charged copolymer-modified sorbents exhibited S-shaped isotherms in most cases, indicative of unrestricted multilayer adsorption.
33448225	3	44	from	increase	614:621	arg1	temperature					652:662	the applied environmental temperature	626:662	the applied environmental temperature	626:662	The adsorption capacity of the negatively charged copolymer-modified sorbents increased with an increase in the applied environmental temperature due to increased protein-sorbent surface hydrophobic and electrostatic interactions.
33448225	4	45	theme	copolymer-grafted	888:904	arg1	sorbents					906:913	the negatively charged copolymer-grafted sorbents	865:913	the negatively charged copolymer-grafted sorbents at both 20 and 50 °C due to electrostatic repulsive effects	865:973	The affinity of the hIgG2 mAb for a positively charged copolymer-grafted sorbent was much lower than that found for the negatively charged copolymer-grafted sorbents at both 20 and 50 °C due to electrostatic repulsive effects.
33448225	4	46	theme	charged	880:886	arg1	sorbents					906:913	the negatively charged copolymer-grafted sorbents	865:913	the negatively charged copolymer-grafted sorbents at both 20 and 50 °C due to electrostatic repulsive effects	865:973	The affinity of the hIgG2 mAb for a positively charged copolymer-grafted sorbent was much lower than that found for the negatively charged copolymer-grafted sorbents at both 20 and 50 °C due to electrostatic repulsive effects.
33448225	3	47	theme	surface	697:703	arg1	interactions					735:746	protein-sorbent surface hydrophobic and electrostatic interactions	681:746	increased protein-sorbent surface hydrophobic and electrostatic interactions	671:746	The adsorption capacity of the negatively charged copolymer-modified sorbents increased with an increase in the applied environmental temperature due to increased protein-sorbent surface hydrophobic and electrostatic interactions.
33448225	1	48	with	sorbents	258:265	arg1	compositions					282:293	different compositions	272:293	different compositions of grafted copolymers	272:315	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers is described.
33448225	2	49	theme	protein	339:345	arg1	loadings					347:354	high protein loadings	334:354	high protein loadings	334:354	At high protein loadings, the adsorption with negatively charged copolymer-modified sorbents exhibited S-shaped isotherms in most cases, indicative of unrestricted multilayer adsorption.
33448225	3	50	theme	hydrophobic	705:715	arg1	interactions					735:746	protein-sorbent surface hydrophobic and electrostatic interactions	681:746	increased protein-sorbent surface hydrophobic and electrostatic interactions	671:746	The adsorption capacity of the negatively charged copolymer-modified sorbents increased with an increase in the applied environmental temperature due to increased protein-sorbent surface hydrophobic and electrostatic interactions.
33448225	2	51	from	isotherms	443:451	arg1	cases					461:465	most cases	456:465	most cases	456:465	At high protein loadings, the adsorption with negatively charged copolymer-modified sorbents exhibited S-shaped isotherms in most cases, indicative of unrestricted multilayer adsorption.
33448225	2	52	theme	high	334:337	arg1	loadings					347:354	high protein loadings	334:354	high protein loadings	334:354	At high protein loadings, the adsorption with negatively charged copolymer-modified sorbents exhibited S-shaped isotherms in most cases, indicative of unrestricted multilayer adsorption.
33448225	1	53	theme	-modified	228:236	arg1	sorbents					258:265	thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents	198:265	thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers	198:315	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers is described.
33448225	4	54	theme	hIgG2	769:773	arg1	mAb					775:777	the hIgG2 mAb	765:777	the hIgG2 mAb	765:777	The affinity of the hIgG2 mAb for a positively charged copolymer-grafted sorbent was much lower than that found for the negatively charged copolymer-grafted sorbents at both 20 and 50 °C due to electrostatic repulsive effects.
33448225	2	55	theme	S-shaped	434:441	arg1	isotherms					443:451	S-shaped isotherms	434:451	S-shaped isotherms	434:451	At high protein loadings, the adsorption with negatively charged copolymer-modified sorbents exhibited S-shaped isotherms in most cases, indicative of unrestricted multilayer adsorption.
33448225	3	56	theme	increased	671:679	arg1	interactions					735:746	protein-sorbent surface hydrophobic and electrostatic interactions	681:746	increased protein-sorbent surface hydrophobic and electrostatic interactions	671:746	The adsorption capacity of the negatively charged copolymer-modified sorbents increased with an increase in the applied environmental temperature due to increased protein-sorbent surface hydrophobic and electrostatic interactions.
33448225	2	57	theme	most	456:459	arg1	cases					461:465	most cases	456:465	most cases	456:465	At high protein loadings, the adsorption with negatively charged copolymer-modified sorbents exhibited S-shaped isotherms in most cases, indicative of unrestricted multilayer adsorption.
33448225	1	58	theme	humanized	151:159	arg1	antibody					172:179	humanized monoclonal antibody	151:179	a humanized monoclonal antibody (hIgG2 mAb)	149:191	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers is described.
33448225	1	58	theme	humanized	151:159	arg1	mAb					188:190	hIgG2 mAb	182:190	hIgG2 mAb	182:190	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers is described.
33448225	3	59	theme	adsorption	522:531	arg1	capacity					533:540	The adsorption capacity	518:540	The adsorption capacity of the negatively charged copolymer-modified sorbents	518:594	The adsorption capacity of the negatively charged copolymer-modified sorbents increased with an increase in the applied environmental temperature due to increased protein-sorbent surface hydrophobic and electrostatic interactions.
33448225	1	60	theme	Sepharose	238:246	arg1	sorbents					258:265	thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents	198:265	thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers	198:315	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers is described.
33448225	5	61	theme	adsorption	1091:1100	arg1	behavior					1102:1109	the adsorption behavior	1087:1109	the adsorption behavior of this humanized mAb	1087:1131	This study has documented that the molecular functionalities of the grafted copolymer can significantly affect the adsorption behavior of this humanized mAb at both 20 and 50 °C with the isothermal dependencies revealing subtle effects due to copolymer composition.
33448225	3	62	theme	protein-sorbent	681:695	arg1	interactions					735:746	protein-sorbent surface hydrophobic and electrostatic interactions	681:746	increased protein-sorbent surface hydrophobic and electrostatic interactions	671:746	The adsorption capacity of the negatively charged copolymer-modified sorbents increased with an increase in the applied environmental temperature due to increased protein-sorbent surface hydrophobic and electrostatic interactions.
33448225	1	63	theme	monoclonal	161:170	arg1	antibody					172:179	humanized monoclonal antibody	151:179	a humanized monoclonal antibody (hIgG2 mAb)	149:191	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers is described.
33448225	1	63	theme	monoclonal	161:170	arg1	mAb					188:190	hIgG2 mAb	182:190	hIgG2 mAb	182:190	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers is described.
33448225	1	64	theme	Fast	248:251	arg1	sorbents					258:265	thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents	198:265	thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers	198:315	The batch adsorption behavior of a humanized monoclonal antibody (hIgG2 mAb) with thermoresponsive polymer (TRP)-modified Sepharose Fast Flow sorbents with different compositions of grafted copolymers is described.
33125939	1	0	theme	potential	118:126	arg1	pulp					102:105	Banana's pseudostem pulp	82:105	Banana's pseudostem pulp (BPP)	82:111	Banana's pseudostem pulp (BPP) is a potential by-product obtained in the mechanical fiber extraction of banana's pseudostem.
33125939	1	0	theme	potential	118:126	arg1	by-product					128:137	a potential by-product	116:137	a potential by-product obtained in the mechanical fiber extraction of banana's pseudostem	116:204	Banana's pseudostem pulp (BPP) is a potential by-product obtained in the mechanical fiber extraction of banana's pseudostem.
33125939	6	1	theme	increasing	967:976	arg1	severity					978:985	increasing severity	967:985	increasing severity	967:985	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	0	2	theme	pseudostem	65:74	arg1	pulp					76:79	banana's pseudostem pulp	56:79	banana's pseudostem pulp	56:79	Assessment of the effect of autohydrolysis treatment in banana's pseudostem pulp.
33125939	5	3	dep	%	871:871	arg1	%					902:902	up to 90%	894:902	73% for the raw material up to 90% for the most severe conditions	869:933	Autohydrolysis pretreatment effectively disrupted the structure of the material, inducing an improvement of the enzymatic digestibility from 73% for the raw material up to 90% for the most severe conditions.
33125939	2	4	from	content	310:316	arg1	lignin					337:342	lignin	337:342	lignin	337:342	Its chemical characterization revealed to have an interesting composition, with a high polysaccharides content and low content in lignin, which makes it particularly relevant for the biorefinery's biochemical platform.
33125939	6	5	theme	oligosaccharides	1044:1059	arg1	%					1144:1144	up to 97%	1136:1144	up to 97%	1136:1144	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	6	5	theme	oligosaccharides	1044:1059	arg1	material					1077:1084	32 g/100 g raw material	1062:1084	32 g/100 g raw material	1062:1084	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	6	5	theme	oligosaccharides	1044:1059	arg1	solid					1129:1133	solid	1129:1133	solid	1129:1133	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	6	5	theme	oligosaccharides	1044:1059	arg1	oligosaccharides					1044:1059	oligosaccharides	1044:1059	oligosaccharides (32 g/100 g raw material)	1044:1085	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	6	5	theme	oligosaccharides	1044:1059	arg1	digestibility					1098:1110	higher digestibility	1091:1110	higher digestibility of the remaining solid (up to 97%)	1091:1145	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	6	5	theme	oligosaccharides	1044:1059	arg1	amount					1034:1039	a higher amount	1025:1039	a higher amount of oligosaccharides (32 g/100 g raw material)	1025:1085	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	0	6	from	Assessment	0:9	arg1	pulp					76:79	banana's pseudostem pulp	56:79	banana's pseudostem pulp	56:79	Assessment of the effect of autohydrolysis treatment in banana's pseudostem pulp.
33125939	5	7	dep	90	900:901	arg1	to					897:898	to	897:898	to	897:898	Autohydrolysis pretreatment effectively disrupted the structure of the material, inducing an improvement of the enzymatic digestibility from 73% for the raw material up to 90% for the most severe conditions.
33125939	4	8	theme	2.3	723:725	arg1	factor					713:718	a severity factor	702:718	a severity factor of 2.3	702:725	The highest oligosaccharides production (24 g/100 g raw material) was obtained at a severity factor of 2.3.
33125939	6	9	theme	stage	940:944	arg1	autohydrolysis					946:959	Two stage autohydrolysis	936:959	Two stage autohydrolysis	936:959	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	3	10	dep	isothermal	469:478	arg1	°C					485:486	140 °C	481:486	140 °C	481:486	Autohydrolysis pretreatment, studied under isothermal (140 °C) and non-isothermal conditions (140-220 °C), yielded oligosaccharides, mainly gluco-oligosaccharides, as the main soluble products.
33125939	2	11	theme	interesting	257:267	arg1	composition					269:279	an interesting composition	254:279	an interesting composition	254:279	Its chemical characterization revealed to have an interesting composition, with a high polysaccharides content and low content in lignin, which makes it particularly relevant for the biorefinery's biochemical platform.
33125939	3	12	theme	isothermal	469:478	arg1	conditions					508:517	isothermal (140 °C) and non-isothermal conditions	469:517	isothermal (140 °C) and non-isothermal conditions (140-220 °C)	469:530	Autohydrolysis pretreatment, studied under isothermal (140 °C) and non-isothermal conditions (140-220 °C), yielded oligosaccharides, mainly gluco-oligosaccharides, as the main soluble products.
33125939	3	12	theme	isothermal	469:478	arg1	°C					528:529	140-220 °C	520:529	140-220 °C	520:529	Autohydrolysis pretreatment, studied under isothermal (140 °C) and non-isothermal conditions (140-220 °C), yielded oligosaccharides, mainly gluco-oligosaccharides, as the main soluble products.
33125939	6	13	dep	97	1142:1143	arg1	to					1139:1140	to	1139:1140	to	1139:1140	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	6	14	theme	solid	1129:1133	arg1	%					1144:1144	up to 97%	1136:1144	up to 97%	1136:1144	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	6	14	theme	solid	1129:1133	arg1	material					1077:1084	32 g/100 g raw material	1062:1084	32 g/100 g raw material	1062:1084	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	6	14	theme	solid	1129:1133	arg1	solid					1129:1133	solid	1129:1133	solid	1129:1133	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	6	14	theme	solid	1129:1133	arg1	oligosaccharides					1044:1059	oligosaccharides	1044:1059	oligosaccharides (32 g/100 g raw material)	1044:1085	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	6	14	theme	solid	1129:1133	arg1	digestibility					1098:1110	higher digestibility	1091:1110	higher digestibility of the remaining solid (up to 97%)	1091:1145	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	6	14	theme	solid	1129:1133	arg1	amount					1034:1039	a higher amount	1025:1039	a higher amount of oligosaccharides (32 g/100 g raw material)	1025:1085	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	5	15	theme	digestibility	850:862	arg1	improvement					821:831	an improvement	818:831	an improvement of the enzymatic digestibility	818:862	Autohydrolysis pretreatment effectively disrupted the structure of the material, inducing an improvement of the enzymatic digestibility from 73% for the raw material up to 90% for the most severe conditions.
33125939	5	16	theme	raw	881:883	arg1	material					885:892	the raw material	877:892	the raw material	877:892	Autohydrolysis pretreatment effectively disrupted the structure of the material, inducing an improvement of the enzymatic digestibility from 73% for the raw material up to 90% for the most severe conditions.
33125939	4	17	theme	oligosaccharides	632:647	arg1	production					649:658	The highest oligosaccharides production	620:658	The highest oligosaccharides production (24 g/100 g raw material)	620:684	The highest oligosaccharides production (24 g/100 g raw material) was obtained at a severity factor of 2.3.
33125939	4	17	theme	oligosaccharides	632:647	arg1	material					676:683	24 g/100 g raw material	661:683	24 g/100 g raw material	661:683	The highest oligosaccharides production (24 g/100 g raw material) was obtained at a severity factor of 2.3.
33125939	5	18	theme	material	799:806	arg1	structure					782:790	the structure	778:790	the structure of the material	778:806	Autohydrolysis pretreatment effectively disrupted the structure of the material, inducing an improvement of the enzymatic digestibility from 73% for the raw material up to 90% for the most severe conditions.
33125939	2	19	from	content	326:332	arg1	lignin					337:342	lignin	337:342	lignin	337:342	Its chemical characterization revealed to have an interesting composition, with a high polysaccharides content and low content in lignin, which makes it particularly relevant for the biorefinery's biochemical platform.
33125939	2	20	theme	high	289:292	arg1	content					310:316	a high polysaccharides content	287:316	a high polysaccharides content	287:316	Its chemical characterization revealed to have an interesting composition, with a high polysaccharides content and low content in lignin, which makes it particularly relevant for the biorefinery's biochemical platform.
33125939	4	21	theme	24 g/100 g	661:670	arg1	production					649:658	The highest oligosaccharides production	620:658	The highest oligosaccharides production (24 g/100 g raw material)	620:684	The highest oligosaccharides production (24 g/100 g raw material) was obtained at a severity factor of 2.3.
33125939	4	21	theme	24 g/100 g	661:670	arg1	material					676:683	24 g/100 g raw material	661:683	24 g/100 g raw material	661:683	The highest oligosaccharides production (24 g/100 g raw material) was obtained at a severity factor of 2.3.
33125939	2	22	contain	have	249:252	arg1	characterization					220:235	Its chemical characterization	207:235	Its chemical characterization	207:235	Its chemical characterization revealed to have an interesting composition, with a high polysaccharides content and low content in lignin, which makes it particularly relevant for the biorefinery's biochemical platform.
33125939	2	22	contain	have	249:252	arg2	composition					269:279	an interesting composition	254:279	an interesting composition	254:279	Its chemical characterization revealed to have an interesting composition, with a high polysaccharides content and low content in lignin, which makes it particularly relevant for the biorefinery's biochemical platform.
33125939	1	23	theme	mechanical	155:164	arg1	extraction					172:181	the mechanical fiber extraction	151:181	the mechanical fiber extraction of banana's pseudostem	151:204	Banana's pseudostem pulp (BPP) is a potential by-product obtained in the mechanical fiber extraction of banana's pseudostem.
33125939	2	24	theme	biochemical	404:414	arg1	platform					416:423	the biorefinery's biochemical platform	386:423	the biorefinery's biochemical platform	386:423	Its chemical characterization revealed to have an interesting composition, with a high polysaccharides content and low content in lignin, which makes it particularly relevant for the biorefinery's biochemical platform.
33125939	6	25	theme	higher	1091:1096	arg1	digestibility					1098:1110	higher digestibility	1091:1110	higher digestibility of the remaining solid (up to 97%)	1091:1145	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	5	26	theme	most	912:915	arg1	conditions					924:933	the most severe conditions	908:933	the most severe conditions	908:933	Autohydrolysis pretreatment effectively disrupted the structure of the material, inducing an improvement of the enzymatic digestibility from 73% for the raw material up to 90% for the most severe conditions.
33125939	6	27	with	autohydrolysis	946:959	arg1	severity					978:985	increasing severity	967:985	increasing severity	967:985	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	1	28	theme	fiber	166:170	arg1	extraction					172:181	the mechanical fiber extraction	151:181	the mechanical fiber extraction of banana's pseudostem	151:204	Banana's pseudostem pulp (BPP) is a potential by-product obtained in the mechanical fiber extraction of banana's pseudostem.
33125939	6	29	theme	raw	1073:1075	arg1	material					1077:1084	32 g/100 g raw material	1062:1084	32 g/100 g raw material	1062:1084	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	6	29	theme	raw	1073:1075	arg1	oligosaccharides					1044:1059	oligosaccharides	1044:1059	oligosaccharides (32 g/100 g raw material)	1044:1085	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	0	30	from	pulp	76:79	arg1	Assessment					0:9	Assessment	0:9	Assessment of the effect of autohydrolysis treatment in banana's pseudostem pulp.	0:80	Assessment of the effect of autohydrolysis treatment in banana's pseudostem pulp.
33125939	1	31	theme	pseudostem	91:100	arg1	BPP					108:110	BPP	108:110	BPP	108:110	Banana's pseudostem pulp (BPP) is a potential by-product obtained in the mechanical fiber extraction of banana's pseudostem.
33125939	1	31	theme	pseudostem	91:100	arg1	pulp					102:105	Banana's pseudostem pulp	82:105	Banana's pseudostem pulp (BPP)	82:111	Banana's pseudostem pulp (BPP) is a potential by-product obtained in the mechanical fiber extraction of banana's pseudostem.
33125939	1	31	theme	pseudostem	91:100	arg1	by-product					128:137	a potential by-product	116:137	a potential by-product obtained in the mechanical fiber extraction of banana's pseudostem	116:204	Banana's pseudostem pulp (BPP) is a potential by-product obtained in the mechanical fiber extraction of banana's pseudostem.
33125939	6	32	theme	32 g/100 g	1062:1071	arg1	material					1077:1084	32 g/100 g raw material	1062:1084	32 g/100 g raw material	1062:1084	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	6	32	theme	32 g/100 g	1062:1071	arg1	oligosaccharides					1044:1059	oligosaccharides	1044:1059	oligosaccharides (32 g/100 g raw material)	1044:1085	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	0	33	theme	autohydrolysis	28:41	arg1	treatment					43:51	autohydrolysis treatment	28:51	autohydrolysis treatment	28:51	Assessment of the effect of autohydrolysis treatment in banana's pseudostem pulp.
33125939	4	34	theme	highest	624:630	arg1	production					649:658	The highest oligosaccharides production	620:658	The highest oligosaccharides production (24 g/100 g raw material)	620:684	The highest oligosaccharides production (24 g/100 g raw material) was obtained at a severity factor of 2.3.
33125939	4	34	theme	highest	624:630	arg1	material					676:683	24 g/100 g raw material	661:683	24 g/100 g raw material	661:683	The highest oligosaccharides production (24 g/100 g raw material) was obtained at a severity factor of 2.3.
33125939	3	35	theme	Autohydrolysis	426:439	arg1	pretreatment					441:452	Autohydrolysis pretreatment	426:452	Autohydrolysis pretreatment	426:452	Autohydrolysis pretreatment, studied under isothermal (140 °C) and non-isothermal conditions (140-220 °C), yielded oligosaccharides, mainly gluco-oligosaccharides, as the main soluble products.
33125939	0	36	theme	effect	18:23	arg1	Assessment					0:9	Assessment	0:9	Assessment of the effect of autohydrolysis treatment in banana's pseudostem pulp.	0:80	Assessment of the effect of autohydrolysis treatment in banana's pseudostem pulp.
33125939	5	37	theme	enzymatic	840:848	arg1	digestibility					850:862	the enzymatic digestibility	836:862	the enzymatic digestibility	836:862	Autohydrolysis pretreatment effectively disrupted the structure of the material, inducing an improvement of the enzymatic digestibility from 73% for the raw material up to 90% for the most severe conditions.
33125939	2	38	theme	low	322:324	arg1	content					326:332	low content	322:332	low content	322:332	Its chemical characterization revealed to have an interesting composition, with a high polysaccharides content and low content in lignin, which makes it particularly relevant for the biorefinery's biochemical platform.
33125939	0	39	theme	treatment	43:51	arg1	effect					18:23	the effect	14:23	the effect of autohydrolysis treatment in banana's pseudostem pulp	14:79	Assessment of the effect of autohydrolysis treatment in banana's pseudostem pulp.
33125939	3	40	theme	main	597:600	arg1	products					610:617	the main soluble products	593:617	the main soluble products	593:617	Autohydrolysis pretreatment, studied under isothermal (140 °C) and non-isothermal conditions (140-220 °C), yielded oligosaccharides, mainly gluco-oligosaccharides, as the main soluble products.
33125939	3	40	theme	main	597:600	arg1	oligosaccharides					541:556	oligosaccharides	541:556	oligosaccharides	541:556	Autohydrolysis pretreatment, studied under isothermal (140 °C) and non-isothermal conditions (140-220 °C), yielded oligosaccharides, mainly gluco-oligosaccharides, as the main soluble products.
33125939	4	41	theme	raw	672:674	arg1	production					649:658	The highest oligosaccharides production	620:658	The highest oligosaccharides production (24 g/100 g raw material)	620:684	The highest oligosaccharides production (24 g/100 g raw material) was obtained at a severity factor of 2.3.
33125939	4	41	theme	raw	672:674	arg1	material					676:683	24 g/100 g raw material	661:683	24 g/100 g raw material	661:683	The highest oligosaccharides production (24 g/100 g raw material) was obtained at a severity factor of 2.3.
33125939	4	42	theme	severity	704:711	arg1	factor					713:718	a severity factor	702:718	a severity factor of 2.3	702:725	The highest oligosaccharides production (24 g/100 g raw material) was obtained at a severity factor of 2.3.
33125939	3	43	theme	soluble	602:608	arg1	products					610:617	the main soluble products	593:617	the main soluble products	593:617	Autohydrolysis pretreatment, studied under isothermal (140 °C) and non-isothermal conditions (140-220 °C), yielded oligosaccharides, mainly gluco-oligosaccharides, as the main soluble products.
33125939	3	43	theme	soluble	602:608	arg1	oligosaccharides					541:556	oligosaccharides	541:556	oligosaccharides	541:556	Autohydrolysis pretreatment, studied under isothermal (140 °C) and non-isothermal conditions (140-220 °C), yielded oligosaccharides, mainly gluco-oligosaccharides, as the main soluble products.
33125939	2	44	theme	polysaccharides	294:308	arg1	content					310:316	a high polysaccharides content	287:316	a high polysaccharides content	287:316	Its chemical characterization revealed to have an interesting composition, with a high polysaccharides content and low content in lignin, which makes it particularly relevant for the biorefinery's biochemical platform.
33125939	1	45	theme	pseudostem	195:204	arg1	extraction					172:181	the mechanical fiber extraction	151:181	the mechanical fiber extraction of banana's pseudostem	151:204	Banana's pseudostem pulp (BPP) is a potential by-product obtained in the mechanical fiber extraction of banana's pseudostem.
33125939	5	46	theme	Autohydrolysis	728:741	arg1	pretreatment					743:754	Autohydrolysis pretreatment	728:754	Autohydrolysis pretreatment	728:754	Autohydrolysis pretreatment effectively disrupted the structure of the material, inducing an improvement of the enzymatic digestibility from 73% for the raw material up to 90% for the most severe conditions.
33125939	5	47	theme	severe	917:922	arg1	conditions					924:933	the most severe conditions	908:933	the most severe conditions	908:933	Autohydrolysis pretreatment effectively disrupted the structure of the material, inducing an improvement of the enzymatic digestibility from 73% for the raw material up to 90% for the most severe conditions.
33125939	6	48	theme	higher	1027:1032	arg1	%					1144:1144	up to 97%	1136:1144	up to 97%	1136:1144	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	6	48	theme	higher	1027:1032	arg1	solid					1129:1133	solid	1129:1133	solid	1129:1133	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	6	48	theme	higher	1027:1032	arg1	material					1077:1084	32 g/100 g raw material	1062:1084	32 g/100 g raw material	1062:1084	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	6	48	theme	higher	1027:1032	arg1	oligosaccharides					1044:1059	oligosaccharides	1044:1059	oligosaccharides (32 g/100 g raw material)	1044:1085	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	6	48	theme	higher	1027:1032	arg1	amount					1034:1039	a higher amount	1025:1039	a higher amount of oligosaccharides (32 g/100 g raw material)	1025:1085	Two stage autohydrolysis, with increasing severity, was also studied, allowing to obtain a higher amount of oligosaccharides (32 g/100 g raw material) and higher digestibility of the remaining solid (up to 97%).
33125939	0	49	from	effect	18:23	arg1	pulp					76:79	banana's pseudostem pulp	56:79	banana's pseudostem pulp	56:79	Assessment of the effect of autohydrolysis treatment in banana's pseudostem pulp.
33125939	2	50	theme	chemical	211:218	arg1	characterization					220:235	Its chemical characterization	207:235	Its chemical characterization	207:235	Its chemical characterization revealed to have an interesting composition, with a high polysaccharides content and low content in lignin, which makes it particularly relevant for the biorefinery's biochemical platform.
33125939	3	51	theme	non-isothermal	493:506	arg1	conditions					508:517	isothermal (140 °C) and non-isothermal conditions	469:517	isothermal (140 °C) and non-isothermal conditions (140-220 °C)	469:530	Autohydrolysis pretreatment, studied under isothermal (140 °C) and non-isothermal conditions (140-220 °C), yielded oligosaccharides, mainly gluco-oligosaccharides, as the main soluble products.
33125939	3	51	theme	non-isothermal	493:506	arg1	°C					528:529	140-220 °C	520:529	140-220 °C	520:529	Autohydrolysis pretreatment, studied under isothermal (140 °C) and non-isothermal conditions (140-220 °C), yielded oligosaccharides, mainly gluco-oligosaccharides, as the main soluble products.
33073381	12	0	theme	structural	1824:1833	arg1	characteristics					1835:1849	structural characteristics	1824:1849	structural characteristics	1824:1849	The chemical components and structural characteristics give ginseng-SDF a noteworthy antioxidant activity and enzyme inhibitory activity in vitro.
33073381	13	1	theme	biological	1964:1973	arg1	activities					1975:1984	biological activities	1964:1984	biological activities	1964:1984	These properties and biological activities indicate that ginseng-SDF has application value in antioxidant and hypoglycemic foods.
33073381	7	2	theme	dietary	1286:1292	arg1	fiber					1294:1298	functional dietary fiber	1275:1298	functional dietary fiber	1275:1298	The results suggested that ginseng residue is a valuable source of functional dietary fiber, and the ginseng-SDF has a potential use in antioxidant and hypoglycemic foods.
33073381	6	3	theme	α-amylase/α-glucosidase	1111:1133	arg1	4.89 mg/ml					1181:1190	4.89 mg/ml	1181:1190	4.89 mg/ml	1181:1190	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	3	theme	α-amylase/α-glucosidase	1111:1133	arg1	activities					915:924	biological activities	904:924	biological activities	904:924	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	3	theme	α-amylase/α-glucosidase	1111:1133	arg1	activities					1146:1155	α-amylase/α-glucosidase inhibitory activities	1111:1155	α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively)	1111:1205	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	3	theme	α-amylase/α-glucosidase	1111:1133	arg1	IC50					1158:1161	IC50	1158:1161	IC50	1158:1161	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	3	theme	α-amylase/α-glucosidase	1111:1133	arg1	6.70 mg/ml					1165:1174	6.70 mg/ml	1165:1174	6.70 mg/ml	1165:1174	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	3	theme	α-amylase/α-glucosidase	1111:1133	arg1	capacity					873:880	oil-holding capacity	861:880	oil-holding capacity (OHC) (3.01 g/g)	861:897	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	3	theme	α-amylase/α-glucosidase	1111:1133	arg1	ginseng-SDF					815:825	ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities	815:924	ginseng-SDF	815:825	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	2	4	theme	dietary	260:266	arg1	fiber					268:272	soluble dietary fiber	252:272	soluble dietary fiber of ginseng (ginseng-SDF, 8.98% content)	252:312	In the present study, soluble dietary fiber of ginseng (ginseng-SDF, 8.98% content) was extracted from ginseng residue, and its physicochemical characterization, structure, and biological activities were studied.
33073381	12	5	theme	antioxidant	1881:1891	arg1	activity					1893:1900	ginseng-SDF a noteworthy antioxidant activity	1856:1900	ginseng-SDF a noteworthy antioxidant activity	1856:1900	The chemical components and structural characteristics give ginseng-SDF a noteworthy antioxidant activity and enzyme inhibitory activity in vitro.
33073381	9	6	from	components	1566:1575	arg1	rich					1506:1509	rich	1506:1509	rich	1506:1509	Ginseng residue is rich in polysaccharides, dietary fiber, proteins, and other components, which is also of great research value.
33073381	3	7	theme	4.42	504:507	arg1	%					508:508	%	508:508	%	508:508	Ginseng-SDF was an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements.
33073381	6	8	theme	good	829:832	arg1	solubility					840:849	a good water solubility	827:849	a good water solubility (98.56%)	827:858	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	8	theme	good	829:832	arg1	%					857:857	98.56%	852:857	98.56%	852:857	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	7	9	theme	valuable	1256:1263	arg1	source					1265:1270	a valuable source	1254:1270	a valuable source of functional dietary fiber	1254:1298	The results suggested that ginseng residue is a valuable source of functional dietary fiber, and the ginseng-SDF has a potential use in antioxidant and hypoglycemic foods.
33073381	7	9	theme	valuable	1256:1263	arg1	residue					1243:1249	ginseng residue	1235:1249	ginseng residue	1235:1249	The results suggested that ginseng residue is a valuable source of functional dietary fiber, and the ginseng-SDF has a potential use in antioxidant and hypoglycemic foods.
33073381	12	10	theme	inhibitory	1913:1922	arg1	activity					1924:1931	enzyme inhibitory activity	1906:1931	enzyme inhibitory activity	1906:1931	The chemical components and structural characteristics give ginseng-SDF a noteworthy antioxidant activity and enzyme inhibitory activity in vitro.
33073381	0	11	theme	enzyme	116:121	arg1	activity					134:141	enzyme inhibitory activity	116:141	enzyme inhibitory activity	116:141	Functional soluble dietary fiber from ginseng residue: Polysaccharide characterization, structure, antioxidant, and enzyme inhibitory activity.
33073381	3	12	theme	rich	519:522	arg1	Ginseng-SDF					443:453	Ginseng-SDF	443:453	Ginseng-SDF	443:453	Ginseng-SDF was an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements.
33073381	3	12	theme	rich	519:522	arg1	heteropolysaccharide					469:488	an acidic heteropolysaccharide	459:488	an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements	459:568	Ginseng-SDF was an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements.
33073381	9	13	theme	dietary	1531:1537	arg1	fiber					1539:1543	dietary fiber	1531:1543	dietary fiber	1531:1543	Ginseng residue is rich in polysaccharides, dietary fiber, proteins, and other components, which is also of great research value.
33073381	7	14	theme	ginseng	1235:1241	arg1	source					1265:1270	a valuable source	1254:1270	a valuable source of functional dietary fiber	1254:1298	The results suggested that ginseng residue is a valuable source of functional dietary fiber, and the ginseng-SDF has a potential use in antioxidant and hypoglycemic foods.
33073381	7	14	theme	ginseng	1235:1241	arg1	residue					1243:1249	ginseng residue	1235:1249	ginseng residue	1235:1249	The results suggested that ginseng residue is a valuable source of functional dietary fiber, and the ginseng-SDF has a potential use in antioxidant and hypoglycemic foods.
33073381	4	15	theme	main	587:590	arg1	composition					607:617	its main monosaccharide composition	583:617	its main monosaccharide composition	583:617	Glucose was its main monosaccharide composition (58.03%).
33073381	1	16	theme	edible	198:203	arg1	medicine					220:227	the most famous edible Chinese herbal medicine	182:227	the most famous edible Chinese herbal medicine	182:227	Ginseng (Panax ginseng C.A. Meyer) is the most famous edible Chinese herbal medicine.
33073381	1	16	theme	edible	198:203	arg1	Ginseng					144:150	Ginseng	144:150	Ginseng (Panax ginseng C.A. Meyer)	144:177	Ginseng (Panax ginseng C.A. Meyer) is the most famous edible Chinese herbal medicine.
33073381	2	17	dep	ginseng	277:283	arg1	content					305:311	8.98% content	299:311	8.98% content	299:311	In the present study, soluble dietary fiber of ginseng (ginseng-SDF, 8.98% content) was extracted from ginseng residue, and its physicochemical characterization, structure, and biological activities were studied.
33073381	2	17	dep	ginseng	277:283	arg1	ginseng-SDF					286:296	ginseng-SDF	286:296	ginseng-SDF	286:296	In the present study, soluble dietary fiber of ginseng (ginseng-SDF, 8.98% content) was extracted from ginseng residue, and its physicochemical characterization, structure, and biological activities were studied.
33073381	3	18	theme	amino	536:540	arg1	acids					542:546	amino acids	536:546	amino acids	536:546	Ginseng-SDF was an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements.
33073381	6	19	theme	oil-holding	861:871	arg1	activity					946:953	the antioxidant activity	930:953	the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively)	930:1046	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	19	theme	oil-holding	861:871	arg1	activities					915:924	biological activities	904:924	biological activities	904:924	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	19	theme	oil-holding	861:871	arg1	activities					1146:1155	α-amylase/α-glucosidase inhibitory activities	1111:1155	α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively)	1111:1205	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	19	theme	oil-holding	861:871	arg1	3.01 g/g					889:896	3.01 g/g	889:896	3.01 g/g	889:896	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	19	theme	oil-holding	861:871	arg1	capacity					873:880	oil-holding capacity	861:880	oil-holding capacity (OHC) (3.01 g/g)	861:897	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	19	theme	oil-holding	861:871	arg1	OHC					883:885	OHC	883:885	OHC	883:885	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	19	theme	oil-holding	861:871	arg1	ginseng-SDF					815:825	ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities	815:924	ginseng-SDF	815:825	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	20	theme	GDRI	1086:1089	arg1	%					1103:1103	GDRI, 33.33%-7.43%	1086:1103	%	1103:1103	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	20	theme	GDRI	1086:1089	arg1	index					1079:1083	glucose diffusion retardation index	1049:1083	glucose diffusion retardation index (GDRI, 33.33%-7.43%)	1049:1104	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	3	21	from	rich	519:522	arg1	acids					542:546	amino acids	536:546	amino acids	536:546	Ginseng-SDF was an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements.
33073381	3	21	from	rich	519:522	arg1	elements					561:568	mineral elements	553:568	mineral elements	553:568	Ginseng-SDF was an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements.
33073381	3	21	from	rich	519:522	arg1	protein					527:533	protein	527:533	protein	527:533	Ginseng-SDF was an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements.
33073381	6	22	theme	diffusion	1057:1065	arg1	%					1103:1103	GDRI, 33.33%-7.43%	1086:1103	%	1103:1103	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	22	theme	diffusion	1057:1065	arg1	index					1079:1083	glucose diffusion retardation index	1049:1083	glucose diffusion retardation index (GDRI, 33.33%-7.43%)	1049:1104	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	9	23	theme	other	1560:1564	arg1	components					1566:1575	other components	1560:1575	other components	1560:1575	Ginseng residue is rich in polysaccharides, dietary fiber, proteins, and other components, which is also of great research value.
33073381	3	24	from	protein	527:533	arg1	rich					519:522	rich	519:522	rich	519:522	Ginseng-SDF was an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements.
33073381	0	25	theme	Polysaccharide	55:68	arg1	characterization					70:85	Polysaccharide characterization	55:85	Polysaccharide characterization	55:85	Functional soluble dietary fiber from ginseng residue: Polysaccharide characterization, structure, antioxidant, and enzyme inhibitory activity.
33073381	3	26	theme	mineral	553:559	arg1	elements					561:568	mineral elements	553:568	mineral elements	553:568	Ginseng-SDF was an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements.
33073381	7	27	contain	has	1321:1323	arg2	use					1337:1339	a potential use	1325:1339	a potential use in antioxidant and hypoglycemic foods	1325:1377	The results suggested that ginseng residue is a valuable source of functional dietary fiber, and the ginseng-SDF has a potential use in antioxidant and hypoglycemic foods.
33073381	7	27	contain	has	1321:1323	arg1	ginseng-SDF					1309:1319	the ginseng-SDF	1305:1319	the ginseng-SDF	1305:1319	The results suggested that ginseng residue is a valuable source of functional dietary fiber, and the ginseng-SDF has a potential use in antioxidant and hypoglycemic foods.
33073381	9	28	from	proteins	1546:1553	arg1	rich					1506:1509	rich	1506:1509	rich	1506:1509	Ginseng residue is rich in polysaccharides, dietary fiber, proteins, and other components, which is also of great research value.
33073381	12	29	theme	a	1868:1868	arg1	activity					1893:1900	ginseng-SDF a noteworthy antioxidant activity	1856:1900	ginseng-SDF a noteworthy antioxidant activity	1856:1900	The chemical components and structural characteristics give ginseng-SDF a noteworthy antioxidant activity and enzyme inhibitory activity in vitro.
33073381	6	30	theme	biological	904:913	arg1	ginseng-SDF					815:825	ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities	815:924	ginseng-SDF	815:825	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	30	theme	biological	904:913	arg1	activity					946:953	the antioxidant activity	930:953	the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively)	930:1046	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	30	theme	biological	904:913	arg1	activities					915:924	biological activities	904:924	biological activities	904:924	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	30	theme	biological	904:913	arg1	activities					1146:1155	α-amylase/α-glucosidase inhibitory activities	1111:1155	α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively)	1111:1205	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	30	theme	biological	904:913	arg1	capacity					873:880	oil-holding capacity	861:880	oil-holding capacity (OHC) (3.01 g/g)	861:897	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	2	31	theme	present	237:243	arg1	study					245:249	the present study	233:249	the present study	233:249	In the present study, soluble dietary fiber of ginseng (ginseng-SDF, 8.98% content) was extracted from ginseng residue, and its physicochemical characterization, structure, and biological activities were studied.
33073381	7	32	theme	fiber	1294:1298	arg1	source					1265:1270	a valuable source	1254:1270	a valuable source of functional dietary fiber	1254:1298	The results suggested that ginseng residue is a valuable source of functional dietary fiber, and the ginseng-SDF has a potential use in antioxidant and hypoglycemic foods.
33073381	7	32	theme	fiber	1294:1298	arg1	residue					1243:1249	ginseng residue	1235:1249	ginseng residue	1235:1249	The results suggested that ginseng residue is a valuable source of functional dietary fiber, and the ginseng-SDF has a potential use in antioxidant and hypoglycemic foods.
33073381	0	33	from	residue	46:52	arg1	fiber					27:31	Functional soluble dietary fiber	0:31	Functional soluble dietary fiber from ginseng residue: Polysaccharide characterization, structure, antioxidant, and enzyme inhibitory activity.	0:142	Functional soluble dietary fiber from ginseng residue: Polysaccharide characterization, structure, antioxidant, and enzyme inhibitory activity.
33073381	5	34	theme	cellulose	680:688	arg1	structure					692:700	a typical cellulose I structure	670:700	a typical cellulose I structure	670:700	Ginseng-SDF had a porous microstructure, a typical cellulose I structure and a large number of hydroxyl functional groups.
33073381	0	35	theme	ginseng	38:44	arg1	residue					46:52	ginseng residue	38:52	ginseng residue	38:52	Functional soluble dietary fiber from ginseng residue: Polysaccharide characterization, structure, antioxidant, and enzyme inhibitory activity.
33073381	2	36	theme	biological	407:416	arg1	activities					418:427	biological activities	407:427	biological activities	407:427	In the present study, soluble dietary fiber of ginseng (ginseng-SDF, 8.98% content) was extracted from ginseng residue, and its physicochemical characterization, structure, and biological activities were studied.
33073381	9	37	theme	research	1601:1608	arg1	value					1610:1614	great research value	1595:1614	great research value	1595:1614	Ginseng residue is rich in polysaccharides, dietary fiber, proteins, and other components, which is also of great research value.
33073381	8	38	theme	PRACTICAL	1380:1388	arg1	APPLICATIONS					1390:1401	PRACTICAL APPLICATIONS	1380:1401	PRACTICAL APPLICATIONS: Ginseng has long been popular as a health food in Asia, North America, and Europe.	1380:1485	PRACTICAL APPLICATIONS: Ginseng has long been popular as a health food in Asia, North America, and Europe.
33073381	0	39	theme	Functional	0:9	arg1	fiber					27:31	Functional soluble dietary fiber	0:31	Functional soluble dietary fiber from ginseng residue: Polysaccharide characterization, structure, antioxidant, and enzyme inhibitory activity.	0:142	Functional soluble dietary fiber from ginseng residue: Polysaccharide characterization, structure, antioxidant, and enzyme inhibitory activity.
33073381	13	40	theme	hypoglycemic	2053:2064	arg1	foods					2066:2070	antioxidant and hypoglycemic foods	2037:2070	antioxidant and hypoglycemic foods	2037:2070	These properties and biological activities indicate that ginseng-SDF has application value in antioxidant and hypoglycemic foods.
33073381	0	41	theme	dietary	19:25	arg1	fiber					27:31	Functional soluble dietary fiber	0:31	Functional soluble dietary fiber from ginseng residue: Polysaccharide characterization, structure, antioxidant, and enzyme inhibitory activity.	0:142	Functional soluble dietary fiber from ginseng residue: Polysaccharide characterization, structure, antioxidant, and enzyme inhibitory activity.
33073381	11	42	theme	dietary	1781:1787	arg1	fiber					1789:1793	functional dietary fiber	1770:1793	functional dietary fiber	1770:1793	The research shows that ginseng residue is a valuable source of functional dietary fiber.
33073381	5	43	contain	had	641:643	arg2	microstructure					654:667	a porous microstructure	645:667	a porous microstructure	645:667	Ginseng-SDF had a porous microstructure, a typical cellulose I structure and a large number of hydroxyl functional groups.
33073381	5	43	contain	had	641:643	arg2	structure					692:700	a typical cellulose I structure	670:700	a typical cellulose I structure	670:700	Ginseng-SDF had a porous microstructure, a typical cellulose I structure and a large number of hydroxyl functional groups.
33073381	5	43	contain	had	641:643	arg1	Ginseng-SDF					629:639	Ginseng-SDF	629:639	Ginseng-SDF	629:639	Ginseng-SDF had a porous microstructure, a typical cellulose I structure and a large number of hydroxyl functional groups.
33073381	5	43	contain	had	641:643	arg2	number					714:719	a large number	706:719	a large number of hydroxyl functional groups	706:749	Ginseng-SDF had a porous microstructure, a typical cellulose I structure and a large number of hydroxyl functional groups.
33073381	2	44	theme	physicochemical	358:372	arg1	characterization					374:389	its physicochemical characterization	354:389	its physicochemical characterization	354:389	In the present study, soluble dietary fiber of ginseng (ginseng-SDF, 8.98% content) was extracted from ginseng residue, and its physicochemical characterization, structure, and biological activities were studied.
33073381	5	45	theme	hydroxyl	724:731	arg1	groups					744:749	hydroxyl functional groups	724:749	hydroxyl functional groups	724:749	Ginseng-SDF had a porous microstructure, a typical cellulose I structure and a large number of hydroxyl functional groups.
33073381	13	46	theme	antioxidant	2037:2047	arg1	foods					2066:2070	antioxidant and hypoglycemic foods	2037:2070	antioxidant and hypoglycemic foods	2037:2070	These properties and biological activities indicate that ginseng-SDF has application value in antioxidant and hypoglycemic foods.
33073381	6	47	theme	structural	783:792	arg1	characteristics					794:808	structural characteristics	783:808	structural characteristics	783:808	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	48	dep	ginseng-SDF	815:825	arg1	solubility					840:849	a good water solubility	827:849	a good water solubility (98.56%)	827:858	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	48	dep	ginseng-SDF	815:825	arg1	%					857:857	98.56%	852:857	98.56%	852:857	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	2	49	theme	8.98	299:302	arg1	%					303:303	%	303:303	%	303:303	In the present study, soluble dietary fiber of ginseng (ginseng-SDF, 8.98% content) was extracted from ginseng residue, and its physicochemical characterization, structure, and biological activities were studied.
33073381	1	50	theme	Chinese	205:211	arg1	medicine					220:227	the most famous edible Chinese herbal medicine	182:227	the most famous edible Chinese herbal medicine	182:227	Ginseng (Panax ginseng C.A. Meyer) is the most famous edible Chinese herbal medicine.
33073381	1	50	theme	Chinese	205:211	arg1	Ginseng					144:150	Ginseng	144:150	Ginseng (Panax ginseng C.A. Meyer)	144:177	Ginseng (Panax ginseng C.A. Meyer) is the most famous edible Chinese herbal medicine.
33073381	11	51	theme	fiber	1789:1793	arg1	source					1760:1765	a valuable source	1749:1765	a valuable source of functional dietary fiber	1749:1793	The research shows that ginseng residue is a valuable source of functional dietary fiber.
33073381	11	51	theme	fiber	1789:1793	arg1	residue					1738:1744	ginseng residue	1730:1744	ginseng residue	1730:1744	The research shows that ginseng residue is a valuable source of functional dietary fiber.
33073381	8	52	theme	health	1439:1444	arg1	food					1446:1449	a health food	1437:1449	a health food in Asia, North America, and Europe	1437:1484	PRACTICAL APPLICATIONS: Ginseng has long been popular as a health food in Asia, North America, and Europe.
33073381	10	53	theme	soluble	1661:1667	arg1	fiber					1677:1681	the soluble dietary fiber	1657:1681	the soluble dietary fiber of ginseng at present	1657:1703	However, there are few studies focus on the soluble dietary fiber of ginseng at present.
33073381	3	54	theme	uronic	491:496	arg1	content					510:516	4.42% content	504:516	4.42% content	504:516	Ginseng-SDF was an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements.
33073381	3	54	theme	uronic	491:496	arg1	acid					498:501	uronic acid	491:501	uronic acid	491:501	Ginseng-SDF was an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements.
33073381	12	55	theme	chemical	1800:1807	arg1	components					1809:1818	The chemical components	1796:1818	The chemical components	1796:1818	The chemical components and structural characteristics give ginseng-SDF a noteworthy antioxidant activity and enzyme inhibitory activity in vitro.
33073381	7	56	theme	potential	1327:1335	arg1	use					1337:1339	a potential use	1325:1339	a potential use in antioxidant and hypoglycemic foods	1325:1377	The results suggested that ginseng residue is a valuable source of functional dietary fiber, and the ginseng-SDF has a potential use in antioxidant and hypoglycemic foods.
33073381	6	57	theme	54.20 μM	987:994	arg1	TE/g					965:968	13.35 μM TE/g	956:968	13.35 μM TE/g	956:968	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	57	theme	54.20 μM	987:994	arg1	TE/g					996:999	54.20 μM TE/g	987:999	54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively	987:1045	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	10	58	theme	ginseng	1686:1692	arg1	fiber					1677:1681	the soluble dietary fiber	1657:1681	the soluble dietary fiber of ginseng at present	1657:1703	However, there are few studies focus on the soluble dietary fiber of ginseng at present.
33073381	6	59	theme	inhibitory	1135:1144	arg1	4.89 mg/ml					1181:1190	4.89 mg/ml	1181:1190	4.89 mg/ml	1181:1190	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	59	theme	inhibitory	1135:1144	arg1	activities					915:924	biological activities	904:924	biological activities	904:924	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	59	theme	inhibitory	1135:1144	arg1	activities					1146:1155	α-amylase/α-glucosidase inhibitory activities	1111:1155	α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively)	1111:1205	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	59	theme	inhibitory	1135:1144	arg1	IC50					1158:1161	IC50	1158:1161	IC50	1158:1161	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	59	theme	inhibitory	1135:1144	arg1	6.70 mg/ml					1165:1174	6.70 mg/ml	1165:1174	6.70 mg/ml	1165:1174	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	59	theme	inhibitory	1135:1144	arg1	capacity					873:880	oil-holding capacity	861:880	oil-holding capacity (OHC) (3.01 g/g)	861:897	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	59	theme	inhibitory	1135:1144	arg1	ginseng-SDF					815:825	ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities	815:924	ginseng-SDF	815:825	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	3	60	theme	%	508:508	arg1	content					510:516	4.42% content	504:516	4.42% content	504:516	Ginseng-SDF was an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements.
33073381	3	60	theme	%	508:508	arg1	acid					498:501	uronic acid	491:501	uronic acid	491:501	Ginseng-SDF was an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements.
33073381	9	61	theme	Ginseng	1487:1493	arg1	residue					1495:1501	Ginseng residue	1487:1501	Ginseng residue	1487:1501	Ginseng residue is rich in polysaccharides, dietary fiber, proteins, and other components, which is also of great research value.
33073381	6	62	theme	glucose	1049:1055	arg1	%					1103:1103	GDRI, 33.33%-7.43%	1086:1103	%	1103:1103	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	62	theme	glucose	1049:1055	arg1	index					1079:1083	glucose diffusion retardation index	1049:1083	glucose diffusion retardation index (GDRI, 33.33%-7.43%)	1049:1104	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	2	63	theme	soluble	252:258	arg1	fiber					268:272	soluble dietary fiber	252:272	soluble dietary fiber of ginseng (ginseng-SDF, 8.98% content)	252:312	In the present study, soluble dietary fiber of ginseng (ginseng-SDF, 8.98% content) was extracted from ginseng residue, and its physicochemical characterization, structure, and biological activities were studied.
33073381	6	64	dep	activities	1146:1155	arg1	6.70 mg/ml					1165:1174	6.70 mg/ml	1165:1174	6.70 mg/ml	1165:1174	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	64	dep	activities	1146:1155	arg1	4.89 mg/ml					1181:1190	4.89 mg/ml	1181:1190	4.89 mg/ml	1181:1190	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	64	dep	activities	1146:1155	arg1	IC50					1158:1161	IC50	1158:1161	IC50	1158:1161	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	64	dep	activities	1146:1155	arg1	activities					1146:1155	α-amylase/α-glucosidase inhibitory activities	1111:1155	α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively)	1111:1205	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	65	theme	water	834:838	arg1	solubility					840:849	a good water solubility	827:849	a good water solubility (98.56%)	827:858	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	65	theme	water	834:838	arg1	%					857:857	98.56%	852:857	98.56%	852:857	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	7	66	theme	functional	1275:1284	arg1	fiber					1294:1298	functional dietary fiber	1275:1298	functional dietary fiber	1275:1298	The results suggested that ginseng residue is a valuable source of functional dietary fiber, and the ginseng-SDF has a potential use in antioxidant and hypoglycemic foods.
33073381	4	67	theme	monosaccharide	592:605	arg1	composition					607:617	its main monosaccharide composition	583:617	its main monosaccharide composition	583:617	Glucose was its main monosaccharide composition (58.03%).
33073381	9	68	from	rich	1506:1509	arg1	proteins					1546:1553	proteins	1546:1553	proteins	1546:1553	Ginseng residue is rich in polysaccharides, dietary fiber, proteins, and other components, which is also of great research value.
33073381	9	68	from	rich	1506:1509	arg1	polysaccharides					1514:1528	polysaccharides	1514:1528	polysaccharides	1514:1528	Ginseng residue is rich in polysaccharides, dietary fiber, proteins, and other components, which is also of great research value.
33073381	9	68	from	rich	1506:1509	arg1	fiber					1539:1543	dietary fiber	1531:1543	dietary fiber	1531:1543	Ginseng residue is rich in polysaccharides, dietary fiber, proteins, and other components, which is also of great research value.
33073381	9	68	from	rich	1506:1509	arg1	components					1566:1575	other components	1560:1575	other components	1560:1575	Ginseng residue is rich in polysaccharides, dietary fiber, proteins, and other components, which is also of great research value.
33073381	0	69	theme	inhibitory	123:132	arg1	activity					134:141	enzyme inhibitory activity	116:141	enzyme inhibitory activity	116:141	Functional soluble dietary fiber from ginseng residue: Polysaccharide characterization, structure, antioxidant, and enzyme inhibitory activity.
33073381	2	70	theme	ginseng	277:283	arg1	fiber					268:272	soluble dietary fiber	252:272	soluble dietary fiber of ginseng (ginseng-SDF, 8.98% content)	252:312	In the present study, soluble dietary fiber of ginseng (ginseng-SDF, 8.98% content) was extracted from ginseng residue, and its physicochemical characterization, structure, and biological activities were studied.
33073381	10	71	from	present	1697:1703	arg1	fiber					1677:1681	the soluble dietary fiber	1657:1681	the soluble dietary fiber of ginseng at present	1657:1703	However, there are few studies focus on the soluble dietary fiber of ginseng at present.
33073381	6	72	theme	FRAP	1021:1024	arg1	assays					1026:1031	FRAP assays	1021:1031	FRAP assays	1021:1031	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	12	73	theme	enzyme	1906:1911	arg1	activity					1924:1931	enzyme inhibitory activity	1906:1931	enzyme inhibitory activity	1906:1931	The chemical components and structural characteristics give ginseng-SDF a noteworthy antioxidant activity and enzyme inhibitory activity in vitro.
33073381	2	74	theme	%	303:303	arg1	content					305:311	8.98% content	299:311	8.98% content	299:311	In the present study, soluble dietary fiber of ginseng (ginseng-SDF, 8.98% content) was extracted from ginseng residue, and its physicochemical characterization, structure, and biological activities were studied.
33073381	2	74	theme	%	303:303	arg1	ginseng-SDF					286:296	ginseng-SDF	286:296	ginseng-SDF	286:296	In the present study, soluble dietary fiber of ginseng (ginseng-SDF, 8.98% content) was extracted from ginseng residue, and its physicochemical characterization, structure, and biological activities were studied.
33073381	5	75	theme	porous	647:652	arg1	microstructure					654:667	a porous microstructure	645:667	a porous microstructure	645:667	Ginseng-SDF had a porous microstructure, a typical cellulose I structure and a large number of hydroxyl functional groups.
33073381	1	76	theme	famous	191:196	arg1	medicine					220:227	the most famous edible Chinese herbal medicine	182:227	the most famous edible Chinese herbal medicine	182:227	Ginseng (Panax ginseng C.A. Meyer) is the most famous edible Chinese herbal medicine.
33073381	1	76	theme	famous	191:196	arg1	Ginseng					144:150	Ginseng	144:150	Ginseng (Panax ginseng C.A. Meyer)	144:177	Ginseng (Panax ginseng C.A. Meyer) is the most famous edible Chinese herbal medicine.
33073381	9	77	from	fiber	1539:1543	arg1	rich					1506:1509	rich	1506:1509	rich	1506:1509	Ginseng residue is rich in polysaccharides, dietary fiber, proteins, and other components, which is also of great research value.
33073381	6	78	theme	%	1097:1097	arg1	%					1103:1103	GDRI, 33.33%-7.43%	1086:1103	%	1103:1103	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	78	theme	%	1097:1097	arg1	index					1079:1083	glucose diffusion retardation index	1049:1083	glucose diffusion retardation index (GDRI, 33.33%-7.43%)	1049:1104	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	11	79	theme	ginseng	1730:1736	arg1	source					1760:1765	a valuable source	1749:1765	a valuable source of functional dietary fiber	1749:1793	The research shows that ginseng residue is a valuable source of functional dietary fiber.
33073381	11	79	theme	ginseng	1730:1736	arg1	residue					1738:1744	ginseng residue	1730:1744	ginseng residue	1730:1744	The research shows that ginseng residue is a valuable source of functional dietary fiber.
33073381	7	80	from	use	1337:1339	arg1	foods					1373:1377	antioxidant and hypoglycemic foods	1344:1377	antioxidant and hypoglycemic foods	1344:1377	The results suggested that ginseng residue is a valuable source of functional dietary fiber, and the ginseng-SDF has a potential use in antioxidant and hypoglycemic foods.
33073381	12	81	theme	noteworthy	1870:1879	arg1	activity					1893:1900	ginseng-SDF a noteworthy antioxidant activity	1856:1900	ginseng-SDF a noteworthy antioxidant activity	1856:1900	The chemical components and structural characteristics give ginseng-SDF a noteworthy antioxidant activity and enzyme inhibitory activity in vitro.
33073381	10	82	theme	few	1636:1638	arg1	studies					1640:1646	few studies	1636:1646	few studies	1636:1646	However, there are few studies focus on the soluble dietary fiber of ginseng at present.
33073381	6	83	theme	antioxidant	934:944	arg1	activity					946:953	the antioxidant activity	930:953	the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively)	930:1046	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	83	theme	antioxidant	934:944	arg1	activities					915:924	biological activities	904:924	biological activities	904:924	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	83	theme	antioxidant	934:944	arg1	ginseng-SDF					815:825	ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities	815:924	ginseng-SDF	815:825	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	83	theme	antioxidant	934:944	arg1	capacity					873:880	oil-holding capacity	861:880	oil-holding capacity (OHC) (3.01 g/g)	861:897	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	5	84	theme	I	690:690	arg1	structure					692:700	a typical cellulose I structure	670:700	a typical cellulose I structure	670:700	Ginseng-SDF had a porous microstructure, a typical cellulose I structure and a large number of hydroxyl functional groups.
33073381	11	85	theme	valuable	1751:1758	arg1	source					1760:1765	a valuable source	1749:1765	a valuable source of functional dietary fiber	1749:1793	The research shows that ginseng residue is a valuable source of functional dietary fiber.
33073381	11	85	theme	valuable	1751:1758	arg1	residue					1738:1744	ginseng residue	1730:1744	ginseng residue	1730:1744	The research shows that ginseng residue is a valuable source of functional dietary fiber.
33073381	6	86	theme	retardation	1067:1077	arg1	%					1103:1103	GDRI, 33.33%-7.43%	1086:1103	%	1103:1103	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	86	theme	retardation	1067:1077	arg1	index					1079:1083	glucose diffusion retardation index	1049:1083	glucose diffusion retardation index (GDRI, 33.33%-7.43%)	1049:1104	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	8	87	dep	APPLICATIONS	1390:1401	arg1	popular					1426:1432	popular	1426:1432	popular	1426:1432	PRACTICAL APPLICATIONS: Ginseng has long been popular as a health food in Asia, North America, and Europe.
33073381	12	88	theme	ginseng-SDF	1856:1866	arg1	activity					1893:1900	ginseng-SDF a noteworthy antioxidant activity	1856:1900	ginseng-SDF a noteworthy antioxidant activity	1856:1900	The chemical components and structural characteristics give ginseng-SDF a noteworthy antioxidant activity and enzyme inhibitory activity in vitro.
33073381	9	89	from	polysaccharides	1514:1528	arg1	rich					1506:1509	rich	1506:1509	rich	1506:1509	Ginseng residue is rich in polysaccharides, dietary fiber, proteins, and other components, which is also of great research value.
33073381	6	90	dep	activity	946:953	arg1	TE/g					965:968	13.35 μM TE/g	956:968	13.35 μM TE/g	956:968	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	90	dep	activity	946:953	arg1	TE/g					981:984	105.17 μM TE/g	971:984	105.17 μM TE/g	971:984	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	90	dep	activity	946:953	arg1	TE/g					996:999	54.20 μM TE/g	987:999	54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively	987:1045	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	5	91	theme	typical	672:678	arg1	structure					692:700	a typical cellulose I structure	670:700	a typical cellulose I structure	670:700	Ginseng-SDF had a porous microstructure, a typical cellulose I structure and a large number of hydroxyl functional groups.
33073381	9	92	theme	great	1595:1599	arg1	value					1610:1614	great research value	1595:1614	great research value	1595:1614	Ginseng residue is rich in polysaccharides, dietary fiber, proteins, and other components, which is also of great research value.
33073381	3	93	from	elements	561:568	arg1	rich					519:522	rich	519:522	rich	519:522	Ginseng-SDF was an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements.
33073381	5	94	theme	functional	733:742	arg1	groups					744:749	hydroxyl functional groups	724:749	hydroxyl functional groups	724:749	Ginseng-SDF had a porous microstructure, a typical cellulose I structure and a large number of hydroxyl functional groups.
33073381	3	95	dep	heteropolysaccharide	469:488	arg1	content					510:516	4.42% content	504:516	4.42% content	504:516	Ginseng-SDF was an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements.
33073381	3	95	dep	heteropolysaccharide	469:488	arg1	acid					498:501	uronic acid	491:501	uronic acid	491:501	Ginseng-SDF was an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements.
33073381	8	96	from	food	1446:1449	arg1	Asia					1454:1457	Asia	1454:1457	Asia	1454:1457	PRACTICAL APPLICATIONS: Ginseng has long been popular as a health food in Asia, North America, and Europe.
33073381	8	96	from	food	1446:1449	arg1	America					1466:1472	America	1466:1472	America	1466:1472	PRACTICAL APPLICATIONS: Ginseng has long been popular as a health food in Asia, North America, and Europe.
33073381	8	96	from	food	1446:1449	arg1	Europe					1479:1484	Europe	1479:1484	Europe	1479:1484	PRACTICAL APPLICATIONS: Ginseng has long been popular as a health food in Asia, North America, and Europe.
33073381	7	97	theme	antioxidant	1344:1354	arg1	foods					1373:1377	antioxidant and hypoglycemic foods	1344:1377	antioxidant and hypoglycemic foods	1344:1377	The results suggested that ginseng residue is a valuable source of functional dietary fiber, and the ginseng-SDF has a potential use in antioxidant and hypoglycemic foods.
33073381	2	98	theme	ginseng	333:339	arg1	residue					341:347	ginseng residue	333:347	ginseng residue	333:347	In the present study, soluble dietary fiber of ginseng (ginseng-SDF, 8.98% content) was extracted from ginseng residue, and its physicochemical characterization, structure, and biological activities were studied.
33073381	5	99	theme	groups	744:749	arg1	structure					692:700	a typical cellulose I structure	670:700	a typical cellulose I structure	670:700	Ginseng-SDF had a porous microstructure, a typical cellulose I structure and a large number of hydroxyl functional groups.
33073381	5	99	theme	groups	744:749	arg1	number					714:719	a large number	706:719	a large number of hydroxyl functional groups	706:749	Ginseng-SDF had a porous microstructure, a typical cellulose I structure and a large number of hydroxyl functional groups.
33073381	5	99	theme	groups	744:749	arg1	microstructure					654:667	a porous microstructure	645:667	a porous microstructure	645:667	Ginseng-SDF had a porous microstructure, a typical cellulose I structure and a large number of hydroxyl functional groups.
33073381	6	100	theme	-7.43	1098:1102	arg1	%					1103:1103	GDRI, 33.33%-7.43%	1086:1103	%	1103:1103	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	6	100	theme	-7.43	1098:1102	arg1	index					1079:1083	glucose diffusion retardation index	1049:1083	glucose diffusion retardation index (GDRI, 33.33%-7.43%)	1049:1104	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	3	101	from	acids	542:546	arg1	rich					519:522	rich	519:522	rich	519:522	Ginseng-SDF was an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements.
33073381	5	102	theme	large	708:712	arg1	number					714:719	a large number	706:719	a large number of hydroxyl functional groups	706:749	Ginseng-SDF had a porous microstructure, a typical cellulose I structure and a large number of hydroxyl functional groups.
33073381	13	103	contain	has	2012:2014	arg1	ginseng-SDF					2000:2010	ginseng-SDF	2000:2010	ginseng-SDF	2000:2010	These properties and biological activities indicate that ginseng-SDF has application value in antioxidant and hypoglycemic foods.
33073381	13	103	contain	has	2012:2014	arg2	value					2028:2032	application value	2016:2032	application value	2016:2032	These properties and biological activities indicate that ginseng-SDF has application value in antioxidant and hypoglycemic foods.
33073381	3	104	theme	acidic	462:467	arg1	Ginseng-SDF					443:453	Ginseng-SDF	443:453	Ginseng-SDF	443:453	Ginseng-SDF was an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements.
33073381	3	104	theme	acidic	462:467	arg1	heteropolysaccharide					469:488	an acidic heteropolysaccharide	459:488	an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements	459:568	Ginseng-SDF was an acidic heteropolysaccharide (uronic acid, 4.42% content) rich in protein, amino acids, and mineral elements.
33073381	7	105	theme	hypoglycemic	1360:1371	arg1	foods					1373:1377	antioxidant and hypoglycemic foods	1344:1377	antioxidant and hypoglycemic foods	1344:1377	The results suggested that ginseng residue is a valuable source of functional dietary fiber, and the ginseng-SDF has a potential use in antioxidant and hypoglycemic foods.
33073381	0	106	dep	fiber	27:31	arg1	activity					134:141	enzyme inhibitory activity	116:141	enzyme inhibitory activity	116:141	Functional soluble dietary fiber from ginseng residue: Polysaccharide characterization, structure, antioxidant, and enzyme inhibitory activity.
33073381	0	106	dep	fiber	27:31	arg1	antioxidant					99:109	antioxidant	99:109	antioxidant	99:109	Functional soluble dietary fiber from ginseng residue: Polysaccharide characterization, structure, antioxidant, and enzyme inhibitory activity.
33073381	0	106	dep	fiber	27:31	arg1	structure					88:96	structure	88:96	structure	88:96	Functional soluble dietary fiber from ginseng residue: Polysaccharide characterization, structure, antioxidant, and enzyme inhibitory activity.
33073381	0	106	dep	fiber	27:31	arg1	characterization					70:85	Polysaccharide characterization	55:85	Polysaccharide characterization	55:85	Functional soluble dietary fiber from ginseng residue: Polysaccharide characterization, structure, antioxidant, and enzyme inhibitory activity.
33073381	10	107	theme	dietary	1669:1675	arg1	fiber					1677:1681	the soluble dietary fiber	1657:1681	the soluble dietary fiber of ginseng at present	1657:1703	However, there are few studies focus on the soluble dietary fiber of ginseng at present.
33073381	11	108	theme	functional	1770:1779	arg1	fiber					1789:1793	functional dietary fiber	1770:1793	functional dietary fiber	1770:1793	The research shows that ginseng residue is a valuable source of functional dietary fiber.
33073381	1	109	theme	herbal	213:218	arg1	medicine					220:227	the most famous edible Chinese herbal medicine	182:227	the most famous edible Chinese herbal medicine	182:227	Ginseng (Panax ginseng C.A. Meyer) is the most famous edible Chinese herbal medicine.
33073381	1	109	theme	herbal	213:218	arg1	Ginseng					144:150	Ginseng	144:150	Ginseng (Panax ginseng C.A. Meyer)	144:177	Ginseng (Panax ginseng C.A. Meyer) is the most famous edible Chinese herbal medicine.
33073381	6	110	theme	chemical	758:765	arg1	composition					767:777	These chemical composition	752:777	These chemical composition	752:777	These chemical composition and structural characteristics gave ginseng-SDF a good water solubility (98.56%), oil-holding capacity (OHC) (3.01 g/g), and biological activities, as the antioxidant activity (13.35 μM TE/g, 105.17 μM TE/g, 54.20 μM TE/g for DPPH, ABTs, and FRAP assays, respectively), glucose diffusion retardation index (GDRI, 33.33%-7.43%), and α-amylase/α-glucosidase inhibitory activities (IC50 , 6.70 mg/ml, and 4.89 mg/ml, respectively).
33073381	4	111	dep	composition	607:617	arg1	%					625:625	58.03%	620:625	58.03%	620:625	Glucose was its main monosaccharide composition (58.03%).
33073381	0	112	theme	soluble	11:17	arg1	fiber					27:31	Functional soluble dietary fiber	0:31	Functional soluble dietary fiber from ginseng residue: Polysaccharide characterization, structure, antioxidant, and enzyme inhibitory activity.	0:142	Functional soluble dietary fiber from ginseng residue: Polysaccharide characterization, structure, antioxidant, and enzyme inhibitory activity.
33073381	13	113	theme	application	2016:2026	arg1	value					2028:2032	application value	2016:2032	application value	2016:2032	These properties and biological activities indicate that ginseng-SDF has application value in antioxidant and hypoglycemic foods.
32565302	8	0	theme	chemical-induced	1525:1540	arg1	hepatotoxicity					1542:1555	chemical-induced hepatotoxicity	1525:1555	chemical-induced hepatotoxicity based on the above biological research	1525:1594	The final results indicated that PPOP could be used in the treatment of chemical-induced hepatotoxicity based on the above biological research.
32565302	6	1	theme	trilaurin	1112:1120	arg1	levels					1007:1012	the levels	1003:1012	the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum	1003:1174	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	8	2	theme	above	1570:1574	arg1	research					1587:1594	the above biological research	1566:1594	the above biological research	1566:1594	The final results indicated that PPOP could be used in the treatment of chemical-induced hepatotoxicity based on the above biological research.
32565302	5	3	theme	Structural	765:774	arg1	analysis					776:783	Structural analysis	765:783	Structural analysis	765:783	Structural analysis indicated that PPOP had multi-strand structure and the absorption peaks of PO and P-O-C.
32565302	0	4	from	characterization	11:26	arg1	injury					151:156	carbon tetrachloride-induced liver injury	116:156	carbon tetrachloride-induced liver injury in mice	116:164	Structural characterization of phosphorylated Pleurotus ostreatus polysaccharide and its hepatoprotective effect on carbon tetrachloride-induced liver injury in mice.
32565302	6	5	from	levels	1007:1012	arg1	serum					1170:1174	the serum	1166:1174	the serum	1166:1174	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	3	6	theme	POP	543:545	arg1	structures					520:529	The structures	516:529	The structures of PPOP and POP	516:545	The structures of PPOP and POP were characterized by FT-IR, ESEM spectroscopy, and Congo red test.
32565302	0	7	theme	tetrachloride-induced	123:143	arg1	injury					151:156	carbon tetrachloride-induced liver injury	116:156	carbon tetrachloride-induced liver injury in mice	116:164	Structural characterization of phosphorylated Pleurotus ostreatus polysaccharide and its hepatoprotective effect on carbon tetrachloride-induced liver injury in mice.
32565302	1	8	theme	male	376:379	arg1	mice					389:392	male Kunming mice	376:392	male Kunming mice	376:392	This study aimed to explore the basic structural features of phosphorylated Pleurotus ostreatus polysaccharide (PPOP) and study the protective effect of PPOP on liver injury induced by carbon tetrachloride in male Kunming mice.
32565302	7	9	theme	antioxidant	1414:1424	arg1	activity					1426:1433	antioxidant activity	1414:1433	antioxidant activity	1414:1433	PPOP exhibited stronger hepatoprotective effect and antioxidant activity in vivo than POP.
32565302	6	10	theme	high-density	1203:1214	arg1	cholesterol					1228:1238	high-density lipoprotein cholesterol	1203:1238	high-density lipoprotein cholesterol	1203:1238	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	4	11	theme	Chemical	615:622	arg1	analysis					636:643	Chemical composition analysis	615:643	Chemical composition analysis	615:643	Chemical composition analysis revealed that PPOP was mainly composed of rhamnose, galacturonic acid, and xylose in a molar ratio of 0.10: 1.98: 1.00.
32565302	0	12	theme	carbon	116:121	arg1	injury					151:156	carbon tetrachloride-induced liver injury	116:156	carbon tetrachloride-induced liver injury in mice	116:164	Structural characterization of phosphorylated Pleurotus ostreatus polysaccharide and its hepatoprotective effect on carbon tetrachloride-induced liver injury in mice.
32565302	1	13	theme	Kunming	381:387	arg1	mice					389:392	male Kunming mice	376:392	male Kunming mice	376:392	This study aimed to explore the basic structural features of phosphorylated Pleurotus ostreatus polysaccharide (PPOP) and study the protective effect of PPOP on liver injury induced by carbon tetrachloride in male Kunming mice.
32565302	3	14	theme	ESEM	576:579	arg1	spectroscopy					581:592	ESEM spectroscopy	576:592	ESEM spectroscopy	576:592	The structures of PPOP and POP were characterized by FT-IR, ESEM spectroscopy, and Congo red test.
32565302	6	15	from	content	1192:1198	arg1	blood					1255:1259	blood	1255:1259	blood	1255:1259	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	1	16	theme	protective	299:308	arg1	effect					310:315	the protective effect	295:315	the protective effect of PPOP on liver injury induced by carbon tetrachloride in male Kunming mice	295:392	This study aimed to explore the basic structural features of phosphorylated Pleurotus ostreatus polysaccharide (PPOP) and study the protective effect of PPOP on liver injury induced by carbon tetrachloride in male Kunming mice.
32565302	6	17	theme	animal	887:892	arg1	experiments					894:904	animal experiments	887:904	animal experiments	887:904	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	5	18	theme	absorption	840:849	arg1	peaks					851:855	the absorption peaks	836:855	the absorption peaks of PO and P-O-C	836:871	Structural analysis indicated that PPOP had multi-strand structure and the absorption peaks of PO and P-O-C.
32565302	2	19	theme	natural	451:457	arg1	polysaccharide					459:472	the natural polysaccharide	447:472	the natural polysaccharide extracted from Pleurotus ostreatus (POP)	447:513	The phosphorylated polysaccharide was prepared from the natural polysaccharide extracted from Pleurotus ostreatus (POP).
32565302	4	20	theme	galacturonic	697:708	arg1	acid					710:713	galacturonic acid	697:713	galacturonic acid	697:713	Chemical composition analysis revealed that PPOP was mainly composed of rhamnose, galacturonic acid, and xylose in a molar ratio of 0.10: 1.98: 1.00.
32565302	5	21	theme	PO	860:861	arg1	peaks					851:855	the absorption peaks	836:855	the absorption peaks of PO and P-O-C	836:871	Structural analysis indicated that PPOP had multi-strand structure and the absorption peaks of PO and P-O-C.
32565302	5	21	theme	PO	860:861	arg1	structure					822:830	multi-strand structure	809:830	multi-strand structure	809:830	Structural analysis indicated that PPOP had multi-strand structure and the absorption peaks of PO and P-O-C.
32565302	0	22	theme	liver	145:149	arg1	injury					151:156	carbon tetrachloride-induced liver injury	116:156	carbon tetrachloride-induced liver injury in mice	116:164	Structural characterization of phosphorylated Pleurotus ostreatus polysaccharide and its hepatoprotective effect on carbon tetrachloride-induced liver injury in mice.
32565302	6	23	theme	lipoprotein	1139:1149	arg1	cholesterol					1151:1161	low-density lipoprotein cholesterol	1127:1161	low-density lipoprotein cholesterol	1127:1161	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	2	24	theme	Pleurotus	489:497	arg1	POP					510:512	POP	510:512	POP	510:512	The phosphorylated polysaccharide was prepared from the natural polysaccharide extracted from Pleurotus ostreatus (POP).
32565302	2	24	theme	Pleurotus	489:497	arg1	ostreatus					499:507	Pleurotus ostreatus	489:507	Pleurotus ostreatus (POP)	489:513	The phosphorylated polysaccharide was prepared from the natural polysaccharide extracted from Pleurotus ostreatus (POP).
32565302	1	25	theme	basic	199:203	arg1	features					216:223	the basic structural features	195:223	the basic structural features of phosphorylated Pleurotus ostreatus polysaccharide (PPOP)	195:283	This study aimed to explore the basic structural features of phosphorylated Pleurotus ostreatus polysaccharide (PPOP) and study the protective effect of PPOP on liver injury induced by carbon tetrachloride in male Kunming mice.
32565302	8	26	theme	biological	1576:1585	arg1	research					1587:1594	the above biological research	1566:1594	the above biological research	1566:1594	The final results indicated that PPOP could be used in the treatment of chemical-induced hepatotoxicity based on the above biological research.
32565302	1	27	from	effect	310:315	arg1	injury					334:339	liver injury	328:339	liver injury induced by carbon tetrachloride in male Kunming mice	328:392	This study aimed to explore the basic structural features of phosphorylated Pleurotus ostreatus polysaccharide (PPOP) and study the protective effect of PPOP on liver injury induced by carbon tetrachloride in male Kunming mice.
32565302	8	28	used	used	1500:1503	arg2	PPOP					1486:1489	PPOP	1486:1489	PPOP	1486:1489	The final results indicated that PPOP could be used in the treatment of chemical-induced hepatotoxicity based on the above biological research.
32565302	4	29	theme	0.10	747:750	arg1	ratio					738:742	a molar ratio	730:742	a molar ratio of 0.10: 1.98: 1.00	730:762	Chemical composition analysis revealed that PPOP was mainly composed of rhamnose, galacturonic acid, and xylose in a molar ratio of 0.10: 1.98: 1.00.
32565302	1	30	theme	structural	205:214	arg1	features					216:223	the basic structural features	195:223	the basic structural features of phosphorylated Pleurotus ostreatus polysaccharide (PPOP)	195:283	This study aimed to explore the basic structural features of phosphorylated Pleurotus ostreatus polysaccharide (PPOP) and study the protective effect of PPOP on liver injury induced by carbon tetrachloride in male Kunming mice.
32565302	0	31	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of phosphorylated Pleurotus ostreatus polysaccharide	0:79	Structural characterization of phosphorylated Pleurotus ostreatus polysaccharide and its hepatoprotective effect on carbon tetrachloride-induced liver injury in mice.
32565302	6	32	theme	malondialdehyde	1286:1300	arg1	content					1275:1281	the content	1271:1281	the content of malondialdehyde	1271:1300	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	3	33	theme	Congo	599:603	arg1	test					609:612	Congo red test	599:612	Congo red test	599:612	The structures of PPOP and POP were characterized by FT-IR, ESEM spectroscopy, and Congo red test.
32565302	2	34	theme	phosphorylated	399:412	arg1	polysaccharide					414:427	The phosphorylated polysaccharide	395:427	The phosphorylated polysaccharide	395:427	The phosphorylated polysaccharide was prepared from the natural polysaccharide extracted from Pleurotus ostreatus (POP).
32565302	0	35	theme	phosphorylated	31:44	arg1	polysaccharide					66:79	phosphorylated Pleurotus ostreatus polysaccharide	31:79	phosphorylated Pleurotus ostreatus polysaccharide	31:79	Structural characterization of phosphorylated Pleurotus ostreatus polysaccharide and its hepatoprotective effect on carbon tetrachloride-induced liver injury in mice.
32565302	5	36	theme	multi-strand	809:820	arg1	structure					822:830	multi-strand structure	809:830	multi-strand structure	809:830	Structural analysis indicated that PPOP had multi-strand structure and the absorption peaks of PO and P-O-C.
32565302	7	37	theme	hepatoprotective	1386:1401	arg1	effect					1403:1408	stronger hepatoprotective effect	1377:1408	stronger hepatoprotective effect	1377:1408	PPOP exhibited stronger hepatoprotective effect and antioxidant activity in vivo than POP.
32565302	6	38	theme	aminotransferase	1053:1068	arg1	levels					1007:1012	the levels	1003:1012	the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum	1003:1174	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	8	39	theme	hepatotoxicity	1542:1555	arg1	treatment					1512:1520	the treatment	1508:1520	the treatment of chemical-induced hepatotoxicity based on the above biological research	1508:1594	The final results indicated that PPOP could be used in the treatment of chemical-induced hepatotoxicity based on the above biological research.
32565302	6	40	theme	alkaline	1071:1078	arg1	phosphatase					1080:1090	alkaline phosphatase	1071:1090	alkaline phosphatase	1071:1090	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	6	41	theme	low-density	1127:1137	arg1	cholesterol					1151:1161	low-density lipoprotein cholesterol	1127:1161	low-density lipoprotein cholesterol	1127:1161	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	1	42	theme	PPOP	320:323	arg1	effect					310:315	the protective effect	295:315	the protective effect of PPOP on liver injury induced by carbon tetrachloride in male Kunming mice	295:392	This study aimed to explore the basic structural features of phosphorylated Pleurotus ostreatus polysaccharide (PPOP) and study the protective effect of PPOP on liver injury induced by carbon tetrachloride in male Kunming mice.
32565302	1	43	theme	phosphorylated	228:241	arg1	PPOP					279:282	PPOP	279:282	PPOP	279:282	This study aimed to explore the basic structural features of phosphorylated Pleurotus ostreatus polysaccharide (PPOP) and study the protective effect of PPOP on liver injury induced by carbon tetrachloride in male Kunming mice.
32565302	1	43	theme	phosphorylated	228:241	arg1	polysaccharide					263:276	phosphorylated Pleurotus ostreatus polysaccharide	228:276	phosphorylated Pleurotus ostreatus polysaccharide (PPOP)	228:283	This study aimed to explore the basic structural features of phosphorylated Pleurotus ostreatus polysaccharide (PPOP) and study the protective effect of PPOP on liver injury induced by carbon tetrachloride in male Kunming mice.
32565302	6	44	theme	liver	962:966	arg1	injury					968:973	liver injury	962:973	liver injury	962:973	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	0	45	from	effect	106:111	arg1	injury					151:156	carbon tetrachloride-induced liver injury	116:156	carbon tetrachloride-induced liver injury in mice	116:164	Structural characterization of phosphorylated Pleurotus ostreatus polysaccharide and its hepatoprotective effect on carbon tetrachloride-induced liver injury in mice.
32565302	6	46	theme	phosphatase	1080:1090	arg1	levels					1007:1012	the levels	1003:1012	the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum	1003:1174	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	1	47	theme	Pleurotus	243:251	arg1	PPOP					279:282	PPOP	279:282	PPOP	279:282	This study aimed to explore the basic structural features of phosphorylated Pleurotus ostreatus polysaccharide (PPOP) and study the protective effect of PPOP on liver injury induced by carbon tetrachloride in male Kunming mice.
32565302	1	47	theme	Pleurotus	243:251	arg1	polysaccharide					263:276	phosphorylated Pleurotus ostreatus polysaccharide	228:276	phosphorylated Pleurotus ostreatus polysaccharide (PPOP)	228:283	This study aimed to explore the basic structural features of phosphorylated Pleurotus ostreatus polysaccharide (PPOP) and study the protective effect of PPOP on liver injury induced by carbon tetrachloride in male Kunming mice.
32565302	0	48	theme	Pleurotus	46:54	arg1	polysaccharide					66:79	phosphorylated Pleurotus ostreatus polysaccharide	31:79	phosphorylated Pleurotus ostreatus polysaccharide	31:79	Structural characterization of phosphorylated Pleurotus ostreatus polysaccharide and its hepatoprotective effect on carbon tetrachloride-induced liver injury in mice.
32565302	1	49	theme	liver	328:332	arg1	injury					334:339	liver injury	328:339	liver injury induced by carbon tetrachloride in male Kunming mice	328:392	This study aimed to explore the basic structural features of phosphorylated Pleurotus ostreatus polysaccharide (PPOP) and study the protective effect of PPOP on liver injury induced by carbon tetrachloride in male Kunming mice.
32565302	5	50	theme	P-O-C	867:871	arg1	peaks					851:855	the absorption peaks	836:855	the absorption peaks of PO and P-O-C	836:871	Structural analysis indicated that PPOP had multi-strand structure and the absorption peaks of PO and P-O-C.
32565302	5	50	theme	P-O-C	867:871	arg1	structure					822:830	multi-strand structure	809:830	multi-strand structure	809:830	Structural analysis indicated that PPOP had multi-strand structure and the absorption peaks of PO and P-O-C.
32565302	6	51	theme	cholesterol	1099:1109	arg1	levels					1007:1012	the levels	1003:1012	the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum	1003:1174	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	6	52	theme	aspartate	1043:1051	arg1	aminotransferase					1053:1068	aspartate aminotransferase	1043:1068	aspartate aminotransferase	1043:1068	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	6	53	from	enzymes	1344:1350	arg1	liver					1355:1359	liver	1355:1359	liver	1355:1359	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	6	54	theme	PPOP	949:952	arg1	effect					939:944	the hepatoprotective effect	918:944	the hepatoprotective effect of PPOP against liver injury	918:973	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	7	55	theme	stronger	1377:1384	arg1	effect					1403:1408	stronger hepatoprotective effect	1377:1408	stronger hepatoprotective effect	1377:1408	PPOP exhibited stronger hepatoprotective effect and antioxidant activity in vivo than POP.
32565302	5	56	contain	had	805:807	arg1	PPOP					800:803	PPOP	800:803	PPOP	800:803	Structural analysis indicated that PPOP had multi-strand structure and the absorption peaks of PO and P-O-C.
32565302	5	56	contain	had	805:807	arg2	structure					822:830	multi-strand structure	809:830	multi-strand structure	809:830	Structural analysis indicated that PPOP had multi-strand structure and the absorption peaks of PO and P-O-C.
32565302	5	56	contain	had	805:807	arg2	peaks					851:855	the absorption peaks	836:855	the absorption peaks of PO and P-O-C	836:871	Structural analysis indicated that PPOP had multi-strand structure and the absorption peaks of PO and P-O-C.
32565302	3	57	theme	red	605:607	arg1	test					609:612	Congo red test	599:612	Congo red test	599:612	The structures of PPOP and POP were characterized by FT-IR, ESEM spectroscopy, and Congo red test.
32565302	6	58	theme	cholesterol	1151:1161	arg1	levels					1007:1012	the levels	1003:1012	the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum	1003:1174	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	0	59	theme	polysaccharide	66:79	arg1	effect					106:111	its hepatoprotective effect	85:111	its hepatoprotective effect on carbon tetrachloride-induced liver injury in mice	85:164	Structural characterization of phosphorylated Pleurotus ostreatus polysaccharide and its hepatoprotective effect on carbon tetrachloride-induced liver injury in mice.
32565302	0	59	theme	polysaccharide	66:79	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of phosphorylated Pleurotus ostreatus polysaccharide	0:79	Structural characterization of phosphorylated Pleurotus ostreatus polysaccharide and its hepatoprotective effect on carbon tetrachloride-induced liver injury in mice.
32565302	6	60	theme	enzymes	1344:1350	arg1	activity					1320:1327	the activity	1316:1327	the activity of antioxidant enzymes in liver	1316:1359	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	0	61	from	injury	151:156	arg1	mice					161:164	mice	161:164	mice	161:164	Structural characterization of phosphorylated Pleurotus ostreatus polysaccharide and its hepatoprotective effect on carbon tetrachloride-induced liver injury in mice.
32565302	0	62	dep	Pleurotus	46:54	arg1	ostreatus					56:64	ostreatus	56:64	ostreatus	56:64	Structural characterization of phosphorylated Pleurotus ostreatus polysaccharide and its hepatoprotective effect on carbon tetrachloride-induced liver injury in mice.
32565302	3	63	theme	PPOP	534:537	arg1	structures					520:529	The structures	516:529	The structures of PPOP and POP	516:545	The structures of PPOP and POP were characterized by FT-IR, ESEM spectroscopy, and Congo red test.
32565302	6	64	theme	hepatoprotective	922:937	arg1	effect					939:944	the hepatoprotective effect	918:944	the hepatoprotective effect of PPOP against liver injury	918:973	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	6	65	theme	antioxidant	1332:1342	arg1	enzymes					1344:1350	antioxidant enzymes	1332:1350	antioxidant enzymes in liver	1332:1359	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	6	66	theme	albumin	1244:1250	arg1	content					1192:1198	the content	1188:1198	the content of high-density lipoprotein cholesterol and albumin in blood	1188:1259	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	1	67	dep	Pleurotus	243:251	arg1	ostreatus					253:261	ostreatus	253:261	ostreatus	253:261	This study aimed to explore the basic structural features of phosphorylated Pleurotus ostreatus polysaccharide (PPOP) and study the protective effect of PPOP on liver injury induced by carbon tetrachloride in male Kunming mice.
32565302	6	68	theme	aminotransferase	1025:1040	arg1	levels					1007:1012	the levels	1003:1012	the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum	1003:1174	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	4	69	dep	0.10	747:750	arg1	1.00					759:762	1.00	759:762	1.00	759:762	Chemical composition analysis revealed that PPOP was mainly composed of rhamnose, galacturonic acid, and xylose in a molar ratio of 0.10: 1.98: 1.00.
32565302	4	69	dep	0.10	747:750	arg1	1.98					753:756	1.98	753:756	1.98	753:756	Chemical composition analysis revealed that PPOP was mainly composed of rhamnose, galacturonic acid, and xylose in a molar ratio of 0.10: 1.98: 1.00.
32565302	1	70	theme	polysaccharide	263:276	arg1	features					216:223	the basic structural features	195:223	the basic structural features of phosphorylated Pleurotus ostreatus polysaccharide (PPOP)	195:283	This study aimed to explore the basic structural features of phosphorylated Pleurotus ostreatus polysaccharide (PPOP) and study the protective effect of PPOP on liver injury induced by carbon tetrachloride in male Kunming mice.
32565302	6	71	from	activity	1320:1327	arg1	liver					1355:1359	liver	1355:1359	liver	1355:1359	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	8	72	theme	final	1457:1461	arg1	results					1463:1469	The final results	1453:1469	The final results	1453:1469	The final results indicated that PPOP could be used in the treatment of chemical-induced hepatotoxicity based on the above biological research.
32565302	4	73	theme	molar	732:736	arg1	ratio					738:742	a molar ratio	730:742	a molar ratio of 0.10: 1.98: 1.00	730:762	Chemical composition analysis revealed that PPOP was mainly composed of rhamnose, galacturonic acid, and xylose in a molar ratio of 0.10: 1.98: 1.00.
32565302	4	74	theme	composition	624:634	arg1	analysis					636:643	Chemical composition analysis	615:643	Chemical composition analysis	615:643	Chemical composition analysis revealed that PPOP was mainly composed of rhamnose, galacturonic acid, and xylose in a molar ratio of 0.10: 1.98: 1.00.
32565302	0	75	theme	hepatoprotective	89:104	arg1	effect					106:111	its hepatoprotective effect	85:111	its hepatoprotective effect on carbon tetrachloride-induced liver injury in mice	85:164	Structural characterization of phosphorylated Pleurotus ostreatus polysaccharide and its hepatoprotective effect on carbon tetrachloride-induced liver injury in mice.
32565302	6	76	theme	cholesterol	1228:1238	arg1	content					1192:1198	the content	1188:1198	the content of high-density lipoprotein cholesterol and albumin in blood	1188:1259	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	6	77	theme	total	1093:1097	arg1	cholesterol					1099:1109	total cholesterol	1093:1109	total cholesterol	1093:1109	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	6	78	theme	alanine	1017:1023	arg1	aminotransferase					1025:1040	alanine aminotransferase	1017:1040	alanine aminotransferase	1017:1040	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	1	79	theme	carbon	352:357	arg1	tetrachloride					359:371	carbon tetrachloride	352:371	carbon tetrachloride	352:371	This study aimed to explore the basic structural features of phosphorylated Pleurotus ostreatus polysaccharide (PPOP) and study the protective effect of PPOP on liver injury induced by carbon tetrachloride in male Kunming mice.
32565302	6	80	theme	lipoprotein	1216:1226	arg1	cholesterol					1228:1238	high-density lipoprotein cholesterol	1203:1238	high-density lipoprotein cholesterol	1203:1238	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
32565302	6	81	from	liver	1355:1359	arg1	activity					1320:1327	the activity	1316:1327	the activity of antioxidant enzymes in liver	1316:1359	Furthermore, animal experiments showed that the hepatoprotective effect of PPOP against liver injury was reflected by decreasing the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total cholesterol, trilaurin, and low-density lipoprotein cholesterol in the serum, increasing the content of high-density lipoprotein cholesterol and albumin in blood, reducing the content of malondialdehyde and promoting the activity of antioxidant enzymes in liver.
33490281	4	0	theme	models	800:805	arg1	effect					674:679	the effect	670:679	the effect of LJP on the intestinal flora	670:710	Then, we studied the effect of LJP on the intestinal flora and immune functions of dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models.
33490281	4	0	theme	models	800:805	arg1	functions					723:731	immune functions	716:731	immune functions of dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models	716:805	Then, we studied the effect of LJP on the intestinal flora and immune functions of dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models.
33490281	7	1	theme	best	1300:1303	arg1	effects					1305:1311	the best effects	1296:1311	the best effects	1296:1311	The results of intestinal flora showed that a high dose (150 mg kg-1) of LJP had the best effects on improving the intestinal probiotics (Bifidobacterium and Lactobacilli) and antagonizing the pathogenic bacteria (Escherichia coli and Enterococcus).
33490281	8	2	theme	lymphocyte	1516:1525	arg1	apoptosis					1527:1535	the spleen lymphocyte apoptosis	1505:1535	the spleen lymphocyte apoptosis	1505:1535	In addition, the measurement results of the spleen lymphocyte apoptosis confirmed from another perspective that LJP had protective effects of immune cells for DSS-treated mice.
33490281	6	3	theme	lymphocyte	1166:1175	arg1	activities					1183:1192	cytotoxic lymphocyte (CTL) activities	1156:1192	cytotoxic lymphocyte (CTL) activities in DSS-treated mice	1156:1212	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	3	4	theme	chromatography	543:556	arg1	method					587:592	a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method	492:592	a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method	492:592	In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP.
33490281	7	5	theme	intestinal	1330:1339	arg1	Lactobacilli					1373:1384	Lactobacilli	1373:1384	Lactobacilli	1373:1384	The results of intestinal flora showed that a high dose (150 mg kg-1) of LJP had the best effects on improving the intestinal probiotics (Bifidobacterium and Lactobacilli) and antagonizing the pathogenic bacteria (Escherichia coli and Enterococcus).
33490281	7	5	theme	intestinal	1330:1339	arg1	Bifidobacterium					1353:1367	Bifidobacterium	1353:1367	Bifidobacterium	1353:1367	The results of intestinal flora showed that a high dose (150 mg kg-1) of LJP had the best effects on improving the intestinal probiotics (Bifidobacterium and Lactobacilli) and antagonizing the pathogenic bacteria (Escherichia coli and Enterococcus).
33490281	7	5	theme	intestinal	1330:1339	arg1	probiotics					1341:1350	the intestinal probiotics	1326:1350	the intestinal probiotics (Bifidobacterium and Lactobacilli)	1326:1385	The results of intestinal flora showed that a high dose (150 mg kg-1) of LJP had the best effects on improving the intestinal probiotics (Bifidobacterium and Lactobacilli) and antagonizing the pathogenic bacteria (Escherichia coli and Enterococcus).
33490281	4	6	theme	induced	767:773	arg1	models					800:805	dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models	736:805	dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models	736:805	Then, we studied the effect of LJP on the intestinal flora and immune functions of dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models.
33490281	6	7	theme	A	1099:1099	arg1	concentration					1108:1120	secretory immunoglobulin A (SIgA) concentration	1074:1120	secretory immunoglobulin A (SIgA) concentration	1074:1120	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	2	8	theme	Lonicera	275:282	arg1	LJP					315:317	LJP	315:317	LJP	315:317	The extraction method of Lonicera japonica Thunb polysaccharide (LJP) has been developed previously by our research group.
33490281	2	8	theme	Lonicera	275:282	arg1	polysaccharide					299:312	Lonicera japonica Thunb polysaccharide	275:312	Lonicera japonica Thunb polysaccharide (LJP)	275:318	The extraction method of Lonicera japonica Thunb polysaccharide (LJP) has been developed previously by our research group.
33490281	8	9	theme	immune	1607:1612	arg1	cells					1614:1618	immune cells	1607:1618	immune cells	1607:1618	In addition, the measurement results of the spleen lymphocyte apoptosis confirmed from another perspective that LJP had protective effects of immune cells for DSS-treated mice.
33490281	4	10	theme	ulcerative	783:792	arg1	models					800:805	dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models	736:805	dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models	736:805	Then, we studied the effect of LJP on the intestinal flora and immune functions of dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models.
33490281	6	11	theme	secretory	1074:1082	arg1	A					1099:1099	secretory immunoglobulin A	1074:1099	secretory immunoglobulin A (SIgA) concentration	1074:1120	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	6	11	theme	secretory	1074:1082	arg1	SIgA					1102:1105	SIgA	1102:1105	SIgA	1102:1105	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	1	12	theme	traditional	176:186	arg1	Thunb					165:169	Lonicera japonica Thunb	147:169	Lonicera japonica Thunb	147:169	Lonicera japonica Thunb is a traditional Chinese herbal medicine for treating intestinal inflammation.
33490281	1	12	theme	traditional	176:186	arg1	medicine					203:210	a traditional Chinese herbal medicine	174:210	a traditional Chinese herbal medicine for treating intestinal inflammation	174:247	Lonicera japonica Thunb is a traditional Chinese herbal medicine for treating intestinal inflammation.
33490281	6	13	theme	natural	1127:1133	arg1	killer					1135:1140	natural killer	1127:1140	natural killer (NK)	1127:1145	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	6	13	theme	natural	1127:1133	arg1	NK					1143:1144	NK	1143:1144	NK	1143:1144	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	1	14	theme	herbal	196:201	arg1	Thunb					165:169	Lonicera japonica Thunb	147:169	Lonicera japonica Thunb	147:169	Lonicera japonica Thunb is a traditional Chinese herbal medicine for treating intestinal inflammation.
33490281	1	14	theme	herbal	196:201	arg1	medicine					203:210	a traditional Chinese herbal medicine	174:210	a traditional Chinese herbal medicine for treating intestinal inflammation	174:247	Lonicera japonica Thunb is a traditional Chinese herbal medicine for treating intestinal inflammation.
33490281	0	15	theme	Dextran	122:128	arg1	Sodium					139:144	Dextran Sulphate Sodium	122:144	Dextran Sulphate Sodium	122:144	Protective Role of a New Polysaccharide Extracted from Lonicera japonica Thunb in Mice with Ulcerative Colitis Induced by Dextran Sulphate Sodium.
33490281	6	16	theme	killer	1135:1140	arg1	cell					1147:1150	the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell	977:1150	the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell	977:1150	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	6	17	theme	DSS-treated	1197:1207	arg1	mice					1209:1212	DSS-treated mice	1197:1212	DSS-treated mice	1197:1212	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	3	18	theme	liquid	536:541	arg1	HPLC					559:562	HPLC	559:562	HPLC	559:562	In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP.
33490281	3	18	theme	liquid	536:541	arg1	chromatography					543:556	precolumn derivatization high-performance liquid chromatography	494:556	a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method	492:592	In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP.
33490281	7	19	theme	intestinal	1230:1239	arg1	flora					1241:1245	intestinal flora	1230:1245	intestinal flora	1230:1245	The results of intestinal flora showed that a high dose (150 mg kg-1) of LJP had the best effects on improving the intestinal probiotics (Bifidobacterium and Lactobacilli) and antagonizing the pathogenic bacteria (Escherichia coli and Enterococcus).
33490281	4	20	from	functions	723:731	arg1	flora					706:710	the intestinal flora	691:710	the intestinal flora	691:710	Then, we studied the effect of LJP on the intestinal flora and immune functions of dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models.
33490281	1	21	theme	japonica	156:163	arg1	Thunb					165:169	Lonicera japonica Thunb	147:169	Lonicera japonica Thunb	147:169	Lonicera japonica Thunb is a traditional Chinese herbal medicine for treating intestinal inflammation.
33490281	1	21	theme	japonica	156:163	arg1	medicine					203:210	a traditional Chinese herbal medicine	174:210	a traditional Chinese herbal medicine for treating intestinal inflammation	174:247	Lonicera japonica Thunb is a traditional Chinese herbal medicine for treating intestinal inflammation.
33490281	4	22	theme	intestinal	695:704	arg1	flora					706:710	the intestinal flora	691:710	the intestinal flora	691:710	Then, we studied the effect of LJP on the intestinal flora and immune functions of dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models.
33490281	2	23	theme	Thunb	293:297	arg1	LJP					315:317	LJP	315:317	LJP	315:317	The extraction method of Lonicera japonica Thunb polysaccharide (LJP) has been developed previously by our research group.
33490281	2	23	theme	Thunb	293:297	arg1	polysaccharide					299:312	Lonicera japonica Thunb polysaccharide	275:312	Lonicera japonica Thunb polysaccharide (LJP)	275:318	The extraction method of Lonicera japonica Thunb polysaccharide (LJP) has been developed previously by our research group.
33490281	0	24	theme	Ulcerative	92:101	arg1	Colitis					103:109	Ulcerative Colitis	92:109	Ulcerative Colitis Induced by Dextran Sulphate Sodium	92:144	Protective Role of a New Polysaccharide Extracted from Lonicera japonica Thunb in Mice with Ulcerative Colitis Induced by Dextran Sulphate Sodium.
33490281	6	25	theme	serum	1002:1006	arg1	factor					1047:1052	tumor necrosis factor	1032:1052	tumor necrosis factor	1032:1052	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	6	25	theme	serum	1002:1006	arg1	interferon-γ					1059:1070	interferon-γ	1059:1070	interferon-γ	1059:1070	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	6	25	theme	serum	1002:1006	arg1	interleukin					1019:1029	interleukin	1019:1029	interleukin	1019:1029	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	6	25	theme	serum	1002:1006	arg1	cytokines					1008:1016	serum cytokines	1002:1016	serum cytokines (interleukin, tumor necrosis factor, and interferon-γ)	1002:1071	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	3	26	dep	transform	398:406	arg1	infrared					408:415	infrared	408:415	transform infrared spectrometer (FT-IR)	398:436	In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP.
33490281	3	27	used	used	442:445	arg2	Fourier					390:396	a Fourier	388:396	a Fourier transform infrared spectrometer (FT-IR)	388:436	In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP.
33490281	0	28	theme	Protective	0:9	arg1	Role					11:14	Protective Role	0:14	Protective Role of a New Polysaccharide	0:38	Protective Role of a New Polysaccharide Extracted from Lonicera japonica Thunb in Mice with Ulcerative Colitis Induced by Dextran Sulphate Sodium.
33490281	7	29	dep	probiotics	1341:1350	arg1	Lactobacilli					1373:1384	Lactobacilli	1373:1384	Lactobacilli	1373:1384	The results of intestinal flora showed that a high dose (150 mg kg-1) of LJP had the best effects on improving the intestinal probiotics (Bifidobacterium and Lactobacilli) and antagonizing the pathogenic bacteria (Escherichia coli and Enterococcus).
33490281	7	29	dep	probiotics	1341:1350	arg1	Bifidobacterium					1353:1367	Bifidobacterium	1353:1367	Bifidobacterium	1353:1367	The results of intestinal flora showed that a high dose (150 mg kg-1) of LJP had the best effects on improving the intestinal probiotics (Bifidobacterium and Lactobacilli) and antagonizing the pathogenic bacteria (Escherichia coli and Enterococcus).
33490281	7	29	dep	probiotics	1341:1350	arg1	probiotics					1341:1350	the intestinal probiotics	1326:1350	the intestinal probiotics (Bifidobacterium and Lactobacilli)	1326:1385	The results of intestinal flora showed that a high dose (150 mg kg-1) of LJP had the best effects on improving the intestinal probiotics (Bifidobacterium and Lactobacilli) and antagonizing the pathogenic bacteria (Escherichia coli and Enterococcus).
33490281	6	30	theme	index	995:999	arg1	cell					1147:1150	the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell	977:1150	the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell	977:1150	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	6	31	theme	concentration	1108:1120	arg1	cell					1147:1150	the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell	977:1150	the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell	977:1150	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	6	32	dep	cytokines	1008:1016	arg1	factor					1047:1052	tumor necrosis factor	1032:1052	tumor necrosis factor	1032:1052	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	6	32	dep	cytokines	1008:1016	arg1	interferon-γ					1059:1070	interferon-γ	1059:1070	interferon-γ	1059:1070	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	6	32	dep	cytokines	1008:1016	arg1	interleukin					1019:1029	interleukin	1019:1029	interleukin	1019:1029	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	6	32	dep	cytokines	1008:1016	arg1	cytokines					1008:1016	serum cytokines	1002:1016	serum cytokines (interleukin, tumor necrosis factor, and interferon-γ)	1002:1071	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	3	33	theme	derivatization	504:517	arg1	HPLC					559:562	HPLC	559:562	HPLC	559:562	In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP.
33490281	3	33	theme	derivatization	504:517	arg1	chromatography					543:556	precolumn derivatization high-performance liquid chromatography	494:556	a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method	492:592	In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP.
33490281	0	34	dep	Lonicera	55:62	arg1	Thunb					73:77	Lonicera japonica Thunb	55:77	Lonicera japonica Thunb in Mice with Ulcerative Colitis Induced by Dextran Sulphate Sodium	55:144	Protective Role of a New Polysaccharide Extracted from Lonicera japonica Thunb in Mice with Ulcerative Colitis Induced by Dextran Sulphate Sodium.
33490281	3	35	used	used	598:601	arg2	method					587:592	a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method	492:592	a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method	492:592	In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP.
33490281	0	36	theme	New	21:23	arg1	Polysaccharide					25:38	a New Polysaccharide	19:38	a New Polysaccharide	19:38	Protective Role of a New Polysaccharide Extracted from Lonicera japonica Thunb in Mice with Ulcerative Colitis Induced by Dextran Sulphate Sodium.
33490281	8	37	theme	apoptosis	1527:1535	arg1	results					1494:1500	the measurement results	1478:1500	the measurement results of the spleen lymphocyte apoptosis	1478:1535	In addition, the measurement results of the spleen lymphocyte apoptosis confirmed from another perspective that LJP had protective effects of immune cells for DSS-treated mice.
33490281	3	38	dep	method	587:592	arg1	Alto					572:575	Alto	572:575	Alto	572:575	In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP.
33490281	3	38	dep	method	587:592	arg1	USA					582:584	USA	582:584	USA	582:584	In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP.
33490281	3	38	dep	method	587:592	arg1	CA					578:579	CA	578:579	CA	578:579	In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP.
33490281	3	39	theme	high-performance	519:534	arg1	HPLC					559:562	HPLC	559:562	HPLC	559:562	In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP.
33490281	3	39	theme	high-performance	519:534	arg1	chromatography					543:556	precolumn derivatization high-performance liquid chromatography	494:556	a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method	492:592	In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP.
33490281	5	40	theme	typical	921:927	arg1	polysaccharides					929:943	typical polysaccharides	921:943	typical polysaccharides	921:943	The results showed that LJP was consisted of 6 types of monosaccharides and had the characteristic absorption of typical polysaccharides.
33490281	6	41	theme	necrosis	1038:1045	arg1	factor					1047:1052	tumor necrosis factor	1032:1052	tumor necrosis factor	1032:1052	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	6	41	theme	necrosis	1038:1045	arg1	cytokines					1008:1016	serum cytokines	1002:1016	serum cytokines (interleukin, tumor necrosis factor, and interferon-γ)	1002:1071	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	7	42	dep	bacteria	1419:1426	arg1	Enterococcus					1450:1461	Enterococcus	1450:1461	Enterococcus	1450:1461	The results of intestinal flora showed that a high dose (150 mg kg-1) of LJP had the best effects on improving the intestinal probiotics (Bifidobacterium and Lactobacilli) and antagonizing the pathogenic bacteria (Escherichia coli and Enterococcus).
33490281	7	42	dep	bacteria	1419:1426	arg1	coli					1441:1444	Escherichia coli	1429:1444	Escherichia coli	1429:1444	The results of intestinal flora showed that a high dose (150 mg kg-1) of LJP had the best effects on improving the intestinal probiotics (Bifidobacterium and Lactobacilli) and antagonizing the pathogenic bacteria (Escherichia coli and Enterococcus).
33490281	8	43	theme	measurement	1482:1492	arg1	results					1494:1500	the measurement results	1478:1500	the measurement results of the spleen lymphocyte apoptosis	1478:1535	In addition, the measurement results of the spleen lymphocyte apoptosis confirmed from another perspective that LJP had protective effects of immune cells for DSS-treated mice.
33490281	2	44	theme	research	357:364	arg1	group					366:370	our research group	353:370	our research group	353:370	The extraction method of Lonicera japonica Thunb polysaccharide (LJP) has been developed previously by our research group.
33490281	6	45	from	cell	1147:1150	arg1	mice					1209:1212	DSS-treated mice	1197:1212	DSS-treated mice	1197:1212	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	4	46	theme	mouse	794:798	arg1	models					800:805	dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models	736:805	dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models	736:805	Then, we studied the effect of LJP on the intestinal flora and immune functions of dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models.
33490281	3	47	theme	LJP	648:650	arg1	composition					633:643	the monosaccharide composition	614:643	the monosaccharide composition of LJP	614:650	In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP.
33490281	2	48	theme	polysaccharide	299:312	arg1	method					265:270	The extraction method	250:270	The extraction method of Lonicera japonica Thunb polysaccharide (LJP)	250:318	The extraction method of Lonicera japonica Thunb polysaccharide (LJP) has been developed previously by our research group.
33490281	8	49	theme	spleen	1509:1514	arg1	apoptosis					1527:1535	the spleen lymphocyte apoptosis	1505:1535	the spleen lymphocyte apoptosis	1505:1535	In addition, the measurement results of the spleen lymphocyte apoptosis confirmed from another perspective that LJP had protective effects of immune cells for DSS-treated mice.
33490281	6	50	theme	cytotoxic	1156:1164	arg1	CTL					1178:1180	CTL	1178:1180	CTL	1178:1180	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	6	50	theme	cytotoxic	1156:1164	arg1	lymphocyte					1166:1175	cytotoxic lymphocyte	1156:1175	cytotoxic lymphocyte (CTL) activities in DSS-treated mice	1156:1212	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	2	51	theme	japonica	284:291	arg1	LJP					315:317	LJP	315:317	LJP	315:317	The extraction method of Lonicera japonica Thunb polysaccharide (LJP) has been developed previously by our research group.
33490281	2	51	theme	japonica	284:291	arg1	polysaccharide					299:312	Lonicera japonica Thunb polysaccharide	275:312	Lonicera japonica Thunb polysaccharide (LJP)	275:318	The extraction method of Lonicera japonica Thunb polysaccharide (LJP) has been developed previously by our research group.
33490281	8	52	theme	protective	1585:1594	arg1	effects					1596:1602	protective effects	1585:1602	protective effects of immune cells for DSS-treated mice	1585:1639	In addition, the measurement results of the spleen lymphocyte apoptosis confirmed from another perspective that LJP had protective effects of immune cells for DSS-treated mice.
33490281	6	53	theme	immunoglobulin	1084:1097	arg1	A					1099:1099	secretory immunoglobulin A	1074:1099	secretory immunoglobulin A (SIgA) concentration	1074:1120	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	6	53	theme	immunoglobulin	1084:1097	arg1	SIgA					1102:1105	SIgA	1102:1105	SIgA	1102:1105	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	4	54	theme	colitis	775:781	arg1	models					800:805	dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models	736:805	dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models	736:805	Then, we studied the effect of LJP on the intestinal flora and immune functions of dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models.
33490281	7	55	theme	LJP	1288:1290	arg1	150 mg kg-1					1272:1282	150 mg kg-1	1272:1282	150 mg kg-1	1272:1282	The results of intestinal flora showed that a high dose (150 mg kg-1) of LJP had the best effects on improving the intestinal probiotics (Bifidobacterium and Lactobacilli) and antagonizing the pathogenic bacteria (Escherichia coli and Enterococcus).
33490281	7	55	theme	LJP	1288:1290	arg1	dose					1266:1269	a high dose	1259:1269	a high dose (150 mg kg-1) of LJP	1259:1290	The results of intestinal flora showed that a high dose (150 mg kg-1) of LJP had the best effects on improving the intestinal probiotics (Bifidobacterium and Lactobacilli) and antagonizing the pathogenic bacteria (Escherichia coli and Enterococcus).
33490281	0	56	with	Mice	82:85	arg1	Colitis					103:109	Ulcerative Colitis	92:109	Ulcerative Colitis Induced by Dextran Sulphate Sodium	92:144	Protective Role of a New Polysaccharide Extracted from Lonicera japonica Thunb in Mice with Ulcerative Colitis Induced by Dextran Sulphate Sodium.
33490281	8	57	theme	cells	1614:1618	arg1	effects					1596:1602	protective effects	1585:1602	protective effects of immune cells for DSS-treated mice	1585:1639	In addition, the measurement results of the spleen lymphocyte apoptosis confirmed from another perspective that LJP had protective effects of immune cells for DSS-treated mice.
33490281	1	58	theme	Chinese	188:194	arg1	Thunb					165:169	Lonicera japonica Thunb	147:169	Lonicera japonica Thunb	147:169	Lonicera japonica Thunb is a traditional Chinese herbal medicine for treating intestinal inflammation.
33490281	1	58	theme	Chinese	188:194	arg1	medicine					203:210	a traditional Chinese herbal medicine	174:210	a traditional Chinese herbal medicine for treating intestinal inflammation	174:247	Lonicera japonica Thunb is a traditional Chinese herbal medicine for treating intestinal inflammation.
33490281	3	59	theme	LJP	484:486	arg1	analysis					472:479	a qualitative analysis	458:479	a qualitative analysis of LJP	458:486	In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP.
33490281	0	60	theme	Sulphate	130:137	arg1	Sodium					139:144	Dextran Sulphate Sodium	122:144	Dextran Sulphate Sodium	122:144	Protective Role of a New Polysaccharide Extracted from Lonicera japonica Thunb in Mice with Ulcerative Colitis Induced by Dextran Sulphate Sodium.
33490281	8	61	contain	had	1581:1583	arg1	LJP					1577:1579	LJP	1577:1579	LJP	1577:1579	In addition, the measurement results of the spleen lymphocyte apoptosis confirmed from another perspective that LJP had protective effects of immune cells for DSS-treated mice.
33490281	8	61	contain	had	1581:1583	arg2	effects					1596:1602	protective effects	1585:1602	protective effects of immune cells for DSS-treated mice	1585:1639	In addition, the measurement results of the spleen lymphocyte apoptosis confirmed from another perspective that LJP had protective effects of immune cells for DSS-treated mice.
33490281	8	62	theme	DSS-treated	1624:1634	arg1	mice					1636:1639	DSS-treated mice	1624:1639	DSS-treated mice	1624:1639	In addition, the measurement results of the spleen lymphocyte apoptosis confirmed from another perspective that LJP had protective effects of immune cells for DSS-treated mice.
33490281	6	63	from	activities	1183:1192	arg1	mice					1209:1212	DSS-treated mice	1197:1212	DSS-treated mice	1197:1212	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	7	64	theme	high	1261:1264	arg1	150 mg kg-1					1272:1282	150 mg kg-1	1272:1282	150 mg kg-1	1272:1282	The results of intestinal flora showed that a high dose (150 mg kg-1) of LJP had the best effects on improving the intestinal probiotics (Bifidobacterium and Lactobacilli) and antagonizing the pathogenic bacteria (Escherichia coli and Enterococcus).
33490281	7	64	theme	high	1261:1264	arg1	dose					1266:1269	a high dose	1259:1269	a high dose (150 mg kg-1) of LJP	1259:1290	The results of intestinal flora showed that a high dose (150 mg kg-1) of LJP had the best effects on improving the intestinal probiotics (Bifidobacterium and Lactobacilli) and antagonizing the pathogenic bacteria (Escherichia coli and Enterococcus).
33490281	2	65	theme	extraction	254:263	arg1	method					265:270	The extraction method	250:270	The extraction method of Lonicera japonica Thunb polysaccharide (LJP)	250:318	The extraction method of Lonicera japonica Thunb polysaccharide (LJP) has been developed previously by our research group.
33490281	7	66	theme	flora	1241:1245	arg1	results					1219:1225	The results	1215:1225	The results of intestinal flora	1215:1245	The results of intestinal flora showed that a high dose (150 mg kg-1) of LJP had the best effects on improving the intestinal probiotics (Bifidobacterium and Lactobacilli) and antagonizing the pathogenic bacteria (Escherichia coli and Enterococcus).
33490281	1	67	theme	Lonicera	147:154	arg1	Thunb					165:169	Lonicera japonica Thunb	147:169	Lonicera japonica Thunb	147:169	Lonicera japonica Thunb is a traditional Chinese herbal medicine for treating intestinal inflammation.
33490281	1	67	theme	Lonicera	147:154	arg1	medicine					203:210	a traditional Chinese herbal medicine	174:210	a traditional Chinese herbal medicine for treating intestinal inflammation	174:247	Lonicera japonica Thunb is a traditional Chinese herbal medicine for treating intestinal inflammation.
33490281	5	68	theme	characteristic	892:905	arg1	absorption					907:916	the characteristic absorption	888:916	the characteristic absorption of typical polysaccharides	888:943	The results showed that LJP was consisted of 6 types of monosaccharides and had the characteristic absorption of typical polysaccharides.
33490281	6	69	theme	cytokines	1008:1016	arg1	cell					1147:1150	the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell	977:1150	the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell	977:1150	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	3	70	dep	Fourier	390:396	arg1	transform					398:406	transform	398:406	transform infrared spectrometer (FT-IR)	398:436	In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP.
33490281	4	71	from	effect	674:679	arg1	flora					706:710	the intestinal flora	691:710	the intestinal flora	691:710	Then, we studied the effect of LJP on the intestinal flora and immune functions of dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models.
33490281	5	72	theme	monosaccharides	864:878	arg1	types					855:859	6 types	853:859	6 types of monosaccharides	853:878	The results showed that LJP was consisted of 6 types of monosaccharides and had the characteristic absorption of typical polysaccharides.
33490281	6	73	theme	organ	989:993	arg1	index					995:999	organ index	989:999	organ index	989:999	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	4	74	theme	LJP	684:686	arg1	effect					674:679	the effect	670:679	the effect of LJP on the intestinal flora	670:710	Then, we studied the effect of LJP on the intestinal flora and immune functions of dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models.
33490281	4	74	theme	LJP	684:686	arg1	functions					723:731	immune functions	716:731	immune functions of dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models	716:805	Then, we studied the effect of LJP on the intestinal flora and immune functions of dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models.
33490281	0	75	theme	Polysaccharide	25:38	arg1	Role					11:14	Protective Role	0:14	Protective Role of a New Polysaccharide	0:38	Protective Role of a New Polysaccharide Extracted from Lonicera japonica Thunb in Mice with Ulcerative Colitis Induced by Dextran Sulphate Sodium.
33490281	6	76	theme	weight	981:986	arg1	cell					1147:1150	the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell	977:1150	the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell	977:1150	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	7	77	contain	had	1292:1294	arg2	effects					1305:1311	the best effects	1296:1311	the best effects	1296:1311	The results of intestinal flora showed that a high dose (150 mg kg-1) of LJP had the best effects on improving the intestinal probiotics (Bifidobacterium and Lactobacilli) and antagonizing the pathogenic bacteria (Escherichia coli and Enterococcus).
33490281	7	77	contain	had	1292:1294	arg1	150 mg kg-1					1272:1282	150 mg kg-1	1272:1282	150 mg kg-1	1272:1282	The results of intestinal flora showed that a high dose (150 mg kg-1) of LJP had the best effects on improving the intestinal probiotics (Bifidobacterium and Lactobacilli) and antagonizing the pathogenic bacteria (Escherichia coli and Enterococcus).
33490281	7	77	contain	had	1292:1294	arg1	dose					1266:1269	a high dose	1259:1269	a high dose (150 mg kg-1) of LJP	1259:1290	The results of intestinal flora showed that a high dose (150 mg kg-1) of LJP had the best effects on improving the intestinal probiotics (Bifidobacterium and Lactobacilli) and antagonizing the pathogenic bacteria (Escherichia coli and Enterococcus).
33490281	7	78	theme	pathogenic	1408:1417	arg1	bacteria					1419:1426	the pathogenic bacteria	1404:1426	the pathogenic bacteria (Escherichia coli and Enterococcus)	1404:1462	The results of intestinal flora showed that a high dose (150 mg kg-1) of LJP had the best effects on improving the intestinal probiotics (Bifidobacterium and Lactobacilli) and antagonizing the pathogenic bacteria (Escherichia coli and Enterococcus).
33490281	3	79	theme	monosaccharide	618:631	arg1	composition					633:643	the monosaccharide composition	614:643	the monosaccharide composition of LJP	614:650	In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP.
33490281	4	80	theme	immune	716:721	arg1	functions					723:731	immune functions	716:731	immune functions of dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models	716:805	Then, we studied the effect of LJP on the intestinal flora and immune functions of dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models.
33490281	3	81	theme	qualitative	460:470	arg1	analysis					472:479	a qualitative analysis	458:479	a qualitative analysis of LJP	458:486	In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP.
33490281	1	82	theme	intestinal	225:234	arg1	inflammation					236:247	intestinal inflammation	225:247	intestinal inflammation	225:247	Lonicera japonica Thunb is a traditional Chinese herbal medicine for treating intestinal inflammation.
33490281	5	83	theme	polysaccharides	929:943	arg1	absorption					907:916	the characteristic absorption	888:916	the characteristic absorption of typical polysaccharides	888:943	The results showed that LJP was consisted of 6 types of monosaccharides and had the characteristic absorption of typical polysaccharides.
33490281	6	84	theme	tumor	1032:1036	arg1	factor					1047:1052	tumor necrosis factor	1032:1052	tumor necrosis factor	1032:1052	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	6	84	theme	tumor	1032:1036	arg1	cytokines					1008:1016	serum cytokines	1002:1016	serum cytokines (interleukin, tumor necrosis factor, and interferon-γ)	1002:1071	LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice.
33490281	3	85	theme	precolumn	494:502	arg1	HPLC					559:562	HPLC	559:562	HPLC	559:562	In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP.
33490281	3	85	theme	precolumn	494:502	arg1	chromatography					543:556	precolumn derivatization high-performance liquid chromatography	494:556	a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method	492:592	In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP.
32865899	0	0	theme	Intracellular	77:89	arg1	Synthesis					106:114	Intracellular Polysaccharide Synthesis	77:114	Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes)	77:193	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	5	1	theme	transcription	910:922	arg1	profile					924:930	gene transcription profile	905:930	gene transcription profile for IPS synthesis of Auricularia auricula-judae under submerged fermentation	905:1007	This is the first exploration of gene transcription profile for IPS synthesis of Auricularia auricula-judae under submerged fermentation.
32865899	6	2	theme	potential	1040:1048	arg1	tool					1050:1053	a potential tool	1038:1053	a potential tool for fermentation process optimization	1038:1091	These results could provide a potential tool for fermentation process optimization and transcriptional regulation of IPS biosynthesis in A. auricula-judae.
32865899	3	3	theme	fermentation	675:686	arg1	process					688:694	the optimized fermentation process	661:694	the optimized fermentation process	661:694	The yield of biomass and IPS were increased by 150% and 177% under the optimized fermentation process, respectively.
32865899	5	4	theme	profile	924:930	arg1	This					872:875	This	872:875	This	872:875	This is the first exploration of gene transcription profile for IPS synthesis of Auricularia auricula-judae under submerged fermentation.
32865899	5	4	theme	profile	924:930	arg1	exploration					890:900	the first exploration	880:900	the first exploration of gene transcription profile for IPS synthesis of Auricularia auricula-judae under submerged fermentation	880:1007	This is the first exploration of gene transcription profile for IPS synthesis of Auricularia auricula-judae under submerged fermentation.
32865899	0	5	theme	Synthesis	106:114	arg1	Process					29:35	Fermentation Process	16:35	Fermentation Process	16:35	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	0	5	theme	Synthesis	106:114	arg1	Impact					45:50	Its Impact	41:50	Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes)	41:193	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	1	6	theme	response	366:373	arg1	methodology					383:393	response surface methodology	366:393	response surface methodology	366:393	To improve the production of mycelial biomass and intracellular polysaccharide (IPS), the culture medium composition and culture condition were respectively optimized by response surface methodology and one-factor-at-a-time methodology.
32865899	4	7	theme	genes	807:811	arg1	transcription					790:802	the transcription	786:802	the transcription of genes involved in IPS synthesis, especially pgm-1, ugp, and pgi	786:869	Moreover, the optimization of fermentation process significantly increased the transcription of genes involved in IPS synthesis, especially pgm-1, ugp, and pgi.
32865899	0	8	from	auricula-judae	163:176	arg1	Process					29:35	Fermentation Process	16:35	Fermentation Process	16:35	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	0	8	from	auricula-judae	163:176	arg1	Impact					45:50	Its Impact	41:50	Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes)	41:193	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	0	9	theme	Polysaccharide	91:104	arg1	Synthesis					106:114	Intracellular Polysaccharide Synthesis	77:114	Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes)	77:193	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	1	10	theme	surface	375:381	arg1	methodology					383:393	response surface methodology	366:393	response surface methodology	366:393	To improve the production of mycelial biomass and intracellular polysaccharide (IPS), the culture medium composition and culture condition were respectively optimized by response surface methodology and one-factor-at-a-time methodology.
32865899	2	11	theme	transcription	565:577	arg1	qRT-PCR					584:590	qRT-PCR	584:590	qRT-PCR	584:590	The impact of fermentation process on the transcription of genes involved in IPS synthesis was evaluated using quantitative reverse transcription PCR (qRT-PCR).
32865899	2	11	theme	transcription	565:577	arg1	PCR					579:581	quantitative reverse transcription PCR	544:581	quantitative reverse transcription PCR (qRT-PCR)	544:591	The impact of fermentation process on the transcription of genes involved in IPS synthesis was evaluated using quantitative reverse transcription PCR (qRT-PCR).
32865899	0	12	from	Impact	45:50	arg1	auricula-judae					163:176	the Wood Ear Medicinal Mushroom Auricularia auricula-judae	119:176	the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes)	119:193	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	0	12	from	Impact	45:50	arg1	Transcription					60:72	Gene Transcription	55:72	Gene Transcription	55:72	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	0	12	from	Impact	45:50	arg1	Agaricomycetes					179:192	Agaricomycetes	179:192	Agaricomycetes	179:192	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	6	13	theme	transcriptional	1097:1111	arg1	regulation					1113:1122	transcriptional regulation	1097:1122	transcriptional regulation of IPS biosynthesis in A. auricula-judae	1097:1163	These results could provide a potential tool for fermentation process optimization and transcriptional regulation of IPS biosynthesis in A. auricula-judae.
32865899	6	14	theme	process	1072:1078	arg1	optimization					1080:1091	fermentation process optimization	1059:1091	fermentation process optimization	1059:1091	These results could provide a potential tool for fermentation process optimization and transcriptional regulation of IPS biosynthesis in A. auricula-judae.
32865899	6	15	theme	IPS	1127:1129	arg1	biosynthesis					1131:1142	IPS biosynthesis	1127:1142	IPS biosynthesis in A. auricula-judae	1127:1163	These results could provide a potential tool for fermentation process optimization and transcriptional regulation of IPS biosynthesis in A. auricula-judae.
32865899	2	16	theme	reverse	557:563	arg1	qRT-PCR					584:590	qRT-PCR	584:590	qRT-PCR	584:590	The impact of fermentation process on the transcription of genes involved in IPS synthesis was evaluated using quantitative reverse transcription PCR (qRT-PCR).
32865899	2	16	theme	reverse	557:563	arg1	PCR					579:581	quantitative reverse transcription PCR	544:581	quantitative reverse transcription PCR (qRT-PCR)	544:591	The impact of fermentation process on the transcription of genes involved in IPS synthesis was evaluated using quantitative reverse transcription PCR (qRT-PCR).
32865899	6	17	theme	A.	1147:1148	arg1	auricula-judae					1150:1163	A. auricula-judae	1147:1163	A. auricula-judae	1147:1163	These results could provide a potential tool for fermentation process optimization and transcriptional regulation of IPS biosynthesis in A. auricula-judae.
32865899	6	18	theme	biosynthesis	1131:1142	arg1	regulation					1113:1122	transcriptional regulation	1097:1122	transcriptional regulation of IPS biosynthesis in A. auricula-judae	1097:1163	These results could provide a potential tool for fermentation process optimization and transcriptional regulation of IPS biosynthesis in A. auricula-judae.
32865899	6	18	theme	biosynthesis	1131:1142	arg1	tool					1050:1053	a potential tool	1038:1053	a potential tool for fermentation process optimization	1038:1091	These results could provide a potential tool for fermentation process optimization and transcriptional regulation of IPS biosynthesis in A. auricula-judae.
32865899	2	19	theme	genes	492:496	arg1	transcription					475:487	the transcription	471:487	the transcription of genes involved in IPS synthesis	471:522	The impact of fermentation process on the transcription of genes involved in IPS synthesis was evaluated using quantitative reverse transcription PCR (qRT-PCR).
32865899	1	20	theme	one-factor-at-a-time	399:418	arg1	methodology					420:430	one-factor-at-a-time methodology	399:430	one-factor-at-a-time methodology	399:430	To improve the production of mycelial biomass and intracellular polysaccharide (IPS), the culture medium composition and culture condition were respectively optimized by response surface methodology and one-factor-at-a-time methodology.
32865899	3	21	theme	biomass	607:613	arg1	yield					598:602	The yield	594:602	The yield of biomass and IPS	594:621	The yield of biomass and IPS were increased by 150% and 177% under the optimized fermentation process, respectively.
32865899	5	22	theme	first	884:888	arg1	This					872:875	This	872:875	This	872:875	This is the first exploration of gene transcription profile for IPS synthesis of Auricularia auricula-judae under submerged fermentation.
32865899	5	22	theme	first	884:888	arg1	exploration					890:900	the first exploration	880:900	the first exploration of gene transcription profile for IPS synthesis of Auricularia auricula-judae under submerged fermentation	880:1007	This is the first exploration of gene transcription profile for IPS synthesis of Auricularia auricula-judae under submerged fermentation.
32865899	6	23	from	regulation	1113:1122	arg1	auricula-judae					1150:1163	A. auricula-judae	1147:1163	A. auricula-judae	1147:1163	These results could provide a potential tool for fermentation process optimization and transcriptional regulation of IPS biosynthesis in A. auricula-judae.
32865899	5	24	theme	auricula-judae	965:978	arg1	synthesis					940:948	IPS synthesis	936:948	IPS synthesis of Auricularia auricula-judae under submerged fermentation	936:1007	This is the first exploration of gene transcription profile for IPS synthesis of Auricularia auricula-judae under submerged fermentation.
32865899	6	25	from	tool	1050:1053	arg1	auricula-judae					1150:1163	A. auricula-judae	1147:1163	A. auricula-judae	1147:1163	These results could provide a potential tool for fermentation process optimization and transcriptional regulation of IPS biosynthesis in A. auricula-judae.
32865899	0	26	theme	Process	29:35	arg1	Optimization					0:11	Optimization	0:11	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).	0:194	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	1	27	theme	culture	286:292	arg1	composition					301:311	the culture medium composition	282:311	the culture medium composition	282:311	To improve the production of mycelial biomass and intracellular polysaccharide (IPS), the culture medium composition and culture condition were respectively optimized by response surface methodology and one-factor-at-a-time methodology.
32865899	0	28	theme	Ear	128:130	arg1	auricula-judae					163:176	the Wood Ear Medicinal Mushroom Auricularia auricula-judae	119:176	the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes)	119:193	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	0	28	theme	Ear	128:130	arg1	Agaricomycetes					179:192	Agaricomycetes	179:192	Agaricomycetes	179:192	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	6	29	from	biosynthesis	1131:1142	arg1	auricula-judae					1150:1163	A. auricula-judae	1147:1163	A. auricula-judae	1147:1163	These results could provide a potential tool for fermentation process optimization and transcriptional regulation of IPS biosynthesis in A. auricula-judae.
32865899	0	30	theme	Fermentation	16:27	arg1	Process					29:35	Fermentation Process	16:35	Fermentation Process	16:35	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	1	31	theme	medium	294:299	arg1	composition					301:311	the culture medium composition	282:311	the culture medium composition	282:311	To improve the production of mycelial biomass and intracellular polysaccharide (IPS), the culture medium composition and culture condition were respectively optimized by response surface methodology and one-factor-at-a-time methodology.
32865899	0	32	theme	Wood	123:126	arg1	auricula-judae					163:176	the Wood Ear Medicinal Mushroom Auricularia auricula-judae	119:176	the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes)	119:193	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	0	32	theme	Wood	123:126	arg1	Agaricomycetes					179:192	Agaricomycetes	179:192	Agaricomycetes	179:192	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	5	33	theme	submerged	986:994	arg1	fermentation					996:1007	submerged fermentation	986:1007	submerged fermentation	986:1007	This is the first exploration of gene transcription profile for IPS synthesis of Auricularia auricula-judae under submerged fermentation.
32865899	2	34	theme	process	460:466	arg1	impact					437:442	The impact	433:442	The impact of fermentation process on the transcription of genes involved in IPS synthesis	433:522	The impact of fermentation process on the transcription of genes involved in IPS synthesis was evaluated using quantitative reverse transcription PCR (qRT-PCR).
32865899	0	35	theme	Mushroom	142:149	arg1	auricula-judae					163:176	the Wood Ear Medicinal Mushroom Auricularia auricula-judae	119:176	the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes)	119:193	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	0	35	theme	Mushroom	142:149	arg1	Agaricomycetes					179:192	Agaricomycetes	179:192	Agaricomycetes	179:192	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	3	36	theme	optimized	665:673	arg1	process					688:694	the optimized fermentation process	661:694	the optimized fermentation process	661:694	The yield of biomass and IPS were increased by 150% and 177% under the optimized fermentation process, respectively.
32865899	2	37	theme	fermentation	447:458	arg1	process					460:466	fermentation process	447:466	fermentation process	447:466	The impact of fermentation process on the transcription of genes involved in IPS synthesis was evaluated using quantitative reverse transcription PCR (qRT-PCR).
32865899	2	38	theme	IPS	510:512	arg1	synthesis					514:522	IPS synthesis	510:522	IPS synthesis	510:522	The impact of fermentation process on the transcription of genes involved in IPS synthesis was evaluated using quantitative reverse transcription PCR (qRT-PCR).
32865899	0	39	theme	Medicinal	132:140	arg1	auricula-judae					163:176	the Wood Ear Medicinal Mushroom Auricularia auricula-judae	119:176	the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes)	119:193	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	0	39	theme	Medicinal	132:140	arg1	Agaricomycetes					179:192	Agaricomycetes	179:192	Agaricomycetes	179:192	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	0	40	from	Transcription	60:72	arg1	Optimization					0:11	Optimization	0:11	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).	0:194	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	1	41	theme	mycelial	225:232	arg1	biomass					234:240	mycelial biomass	225:240	mycelial biomass	225:240	To improve the production of mycelial biomass and intracellular polysaccharide (IPS), the culture medium composition and culture condition were respectively optimized by response surface methodology and one-factor-at-a-time methodology.
32865899	1	42	theme	culture	317:323	arg1	condition					325:333	culture condition	317:333	culture condition	317:333	To improve the production of mycelial biomass and intracellular polysaccharide (IPS), the culture medium composition and culture condition were respectively optimized by response surface methodology and one-factor-at-a-time methodology.
32865899	3	43	theme	IPS	619:621	arg1	yield					598:602	The yield	594:602	The yield of biomass and IPS	594:621	The yield of biomass and IPS were increased by 150% and 177% under the optimized fermentation process, respectively.
32865899	0	44	theme	Impact	45:50	arg1	Optimization					0:11	Optimization	0:11	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).	0:194	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	0	45	theme	Auricularia	151:161	arg1	auricula-judae					163:176	the Wood Ear Medicinal Mushroom Auricularia auricula-judae	119:176	the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes)	119:193	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	0	45	theme	Auricularia	151:161	arg1	Agaricomycetes					179:192	Agaricomycetes	179:192	Agaricomycetes	179:192	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	2	46	from	impact	437:442	arg1	transcription					475:487	the transcription	471:487	the transcription of genes involved in IPS synthesis	471:522	The impact of fermentation process on the transcription of genes involved in IPS synthesis was evaluated using quantitative reverse transcription PCR (qRT-PCR).
32865899	5	47	theme	IPS	936:938	arg1	synthesis					940:948	IPS synthesis	936:948	IPS synthesis of Auricularia auricula-judae under submerged fermentation	936:1007	This is the first exploration of gene transcription profile for IPS synthesis of Auricularia auricula-judae under submerged fermentation.
32865899	4	48	theme	process	754:760	arg1	optimization					725:736	the optimization	721:736	the optimization of fermentation process	721:760	Moreover, the optimization of fermentation process significantly increased the transcription of genes involved in IPS synthesis, especially pgm-1, ugp, and pgi.
32865899	1	49	theme	biomass	234:240	arg1	production					211:220	the production	207:220	the production of mycelial biomass	207:240	To improve the production of mycelial biomass and intracellular polysaccharide (IPS), the culture medium composition and culture condition were respectively optimized by response surface methodology and one-factor-at-a-time methodology.
32865899	1	49	theme	biomass	234:240	arg1	polysaccharide					260:273	intracellular polysaccharide	246:273	intracellular polysaccharide (IPS)	246:279	To improve the production of mycelial biomass and intracellular polysaccharide (IPS), the culture medium composition and culture condition were respectively optimized by response surface methodology and one-factor-at-a-time methodology.
32865899	1	49	theme	biomass	234:240	arg1	IPS					276:278	IPS	276:278	IPS	276:278	To improve the production of mycelial biomass and intracellular polysaccharide (IPS), the culture medium composition and culture condition were respectively optimized by response surface methodology and one-factor-at-a-time methodology.
32865899	0	50	from	Synthesis	106:114	arg1	auricula-judae					163:176	the Wood Ear Medicinal Mushroom Auricularia auricula-judae	119:176	the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes)	119:193	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	0	50	from	Synthesis	106:114	arg1	Agaricomycetes					179:192	Agaricomycetes	179:192	Agaricomycetes	179:192	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	4	51	theme	IPS	825:827	arg1	synthesis					829:837	IPS synthesis	825:837	IPS synthesis	825:837	Moreover, the optimization of fermentation process significantly increased the transcription of genes involved in IPS synthesis, especially pgm-1, ugp, and pgi.
32865899	6	52	from	auricula-judae	1150:1163	arg1	regulation					1113:1122	transcriptional regulation	1097:1122	transcriptional regulation of IPS biosynthesis in A. auricula-judae	1097:1163	These results could provide a potential tool for fermentation process optimization and transcriptional regulation of IPS biosynthesis in A. auricula-judae.
32865899	6	52	from	auricula-judae	1150:1163	arg1	tool					1050:1053	a potential tool	1038:1053	a potential tool for fermentation process optimization	1038:1091	These results could provide a potential tool for fermentation process optimization and transcriptional regulation of IPS biosynthesis in A. auricula-judae.
32865899	4	53	theme	fermentation	741:752	arg1	process					754:760	fermentation process	741:760	fermentation process	741:760	Moreover, the optimization of fermentation process significantly increased the transcription of genes involved in IPS synthesis, especially pgm-1, ugp, and pgi.
32865899	6	54	theme	fermentation	1059:1070	arg1	optimization					1080:1091	fermentation process optimization	1059:1091	fermentation process optimization	1059:1091	These results could provide a potential tool for fermentation process optimization and transcriptional regulation of IPS biosynthesis in A. auricula-judae.
32865899	2	55	theme	quantitative	544:555	arg1	qRT-PCR					584:590	qRT-PCR	584:590	qRT-PCR	584:590	The impact of fermentation process on the transcription of genes involved in IPS synthesis was evaluated using quantitative reverse transcription PCR (qRT-PCR).
32865899	2	55	theme	quantitative	544:555	arg1	PCR					579:581	quantitative reverse transcription PCR	544:581	quantitative reverse transcription PCR (qRT-PCR)	544:591	The impact of fermentation process on the transcription of genes involved in IPS synthesis was evaluated using quantitative reverse transcription PCR (qRT-PCR).
32865899	1	56	theme	intracellular	246:258	arg1	IPS					276:278	IPS	276:278	IPS	276:278	To improve the production of mycelial biomass and intracellular polysaccharide (IPS), the culture medium composition and culture condition were respectively optimized by response surface methodology and one-factor-at-a-time methodology.
32865899	1	56	theme	intracellular	246:258	arg1	polysaccharide					260:273	intracellular polysaccharide	246:273	intracellular polysaccharide (IPS)	246:279	To improve the production of mycelial biomass and intracellular polysaccharide (IPS), the culture medium composition and culture condition were respectively optimized by response surface methodology and one-factor-at-a-time methodology.
32865899	0	57	theme	Gene	55:58	arg1	Transcription					60:72	Gene Transcription	55:72	Gene Transcription	55:72	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	0	58	from	Process	29:35	arg1	auricula-judae					163:176	the Wood Ear Medicinal Mushroom Auricularia auricula-judae	119:176	the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes)	119:193	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	0	58	from	Process	29:35	arg1	Transcription					60:72	Gene Transcription	55:72	Gene Transcription	55:72	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	0	58	from	Process	29:35	arg1	Agaricomycetes					179:192	Agaricomycetes	179:192	Agaricomycetes	179:192	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	0	59	from	Optimization	0:11	arg1	Transcription					60:72	Gene Transcription	55:72	Gene Transcription	55:72	Optimization of Fermentation Process and Its Impact on Gene Transcription of Intracellular Polysaccharide Synthesis in the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).
32865899	5	60	theme	gene	905:908	arg1	profile					924:930	gene transcription profile	905:930	gene transcription profile for IPS synthesis of Auricularia auricula-judae under submerged fermentation	905:1007	This is the first exploration of gene transcription profile for IPS synthesis of Auricularia auricula-judae under submerged fermentation.
33872826	0	0	theme	cancer	120:125	arg1	cells					127:131	gastrointestinal cancer cells	103:131	gastrointestinal cancer cells	103:131	Synthesis of nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel for drug delivery to gastrointestinal cancer cells.
33872826	3	1	theme	scratch	753:759	arg1	assays					761:766	in vitro cytotoxicity, BrdU cell proliferation, and scratch assays	701:766	in vitro cytotoxicity, BrdU cell proliferation, and scratch assays	701:766	in vitro cytotoxicity, BrdU cell proliferation, and scratch assays demonstrate that the nanohydroxyapatite/collagen-loaded fucoidan-based nanomaterial exhibits non-toxicity and increases cell proliferation and migration.
33872826	2	2	theme	transmission	661:672	arg1	microscopy					683:692	transmission electron microscopy	661:692	transmission electron microscopy (TEM)	661:698	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	2	2	theme	transmission	661:672	arg1	TEM					695:697	TEM	695:697	TEM	695:697	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	3	3	dep	cell	888:891	arg1	migration					911:919	migration	911:919	migration	911:919	in vitro cytotoxicity, BrdU cell proliferation, and scratch assays demonstrate that the nanohydroxyapatite/collagen-loaded fucoidan-based nanomaterial exhibits non-toxicity and increases cell proliferation and migration.
33872826	3	3	dep	cell	888:891	arg1	proliferation					893:905	proliferation	893:905	proliferation	893:905	in vitro cytotoxicity, BrdU cell proliferation, and scratch assays demonstrate that the nanohydroxyapatite/collagen-loaded fucoidan-based nanomaterial exhibits non-toxicity and increases cell proliferation and migration.
33872826	2	4	theme	high	452:455	arg1	stability					465:473	a high thermal stability	450:473	a high thermal stability	450:473	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	4	5	theme	fabricated	979:988	arg1	nanomaterial					990:1001	the fabricated nanomaterial	975:1001	the fabricated nanomaterial	975:1001	in vitro free radical scavenging assays confirm that the fabricated nanomaterial inhibits reactive oxygen species and generates singlet oxygen radicals in the gastrointestinal cancer cells by.
33872826	0	6	theme	gastrointestinal	103:118	arg1	cells					127:131	gastrointestinal cancer cells	103:131	gastrointestinal cancer cells	103:131	Synthesis of nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel for drug delivery to gastrointestinal cancer cells.
33872826	3	7	theme	proliferation	734:746	arg1	assays					761:766	in vitro cytotoxicity, BrdU cell proliferation, and scratch assays	701:766	in vitro cytotoxicity, BrdU cell proliferation, and scratch assays	701:766	in vitro cytotoxicity, BrdU cell proliferation, and scratch assays demonstrate that the nanohydroxyapatite/collagen-loaded fucoidan-based nanomaterial exhibits non-toxicity and increases cell proliferation and migration.
33872826	5	8	theme	potential	1184:1192	arg1	carrier					1194:1200	a potential carrier	1182:1200	a potential carrier for the targeted drug delivery to the gastrointestinal cancer cells	1182:1268	The current findings suggest that the proposed nanomaterial can be a potential carrier for the targeted drug delivery to the gastrointestinal cancer cells.
33872826	5	8	theme	potential	1184:1192	arg1	nanomaterial					1162:1173	the proposed nanomaterial	1149:1173	the proposed nanomaterial	1149:1173	The current findings suggest that the proposed nanomaterial can be a potential carrier for the targeted drug delivery to the gastrointestinal cancer cells.
33872826	1	9	theme	nanohydroxyapatite/collagen-loaded	186:219	arg1	hydrogel					246:253	nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel	186:253	nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel	186:253	The present study aims to evaluate the synthesis of nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel and characterized its physico-chemical properties for targeted drug delivery to gastrointestinal cancer cells.
33872826	5	10	theme	gastrointestinal	1240:1255	arg1	cells					1264:1268	the gastrointestinal cancer cells	1236:1268	the gastrointestinal cancer cells	1236:1268	The current findings suggest that the proposed nanomaterial can be a potential carrier for the targeted drug delivery to the gastrointestinal cancer cells.
33872826	1	11	theme	gastrointestinal	335:350	arg1	cells					359:363	gastrointestinal cancer cells	335:363	gastrointestinal cancer cells	335:363	The present study aims to evaluate the synthesis of nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel and characterized its physico-chemical properties for targeted drug delivery to gastrointestinal cancer cells.
33872826	5	12	theme	drug	1219:1222	arg1	delivery					1224:1231	the targeted drug delivery	1206:1231	the targeted drug delivery to the gastrointestinal cancer cells	1206:1268	The current findings suggest that the proposed nanomaterial can be a potential carrier for the targeted drug delivery to the gastrointestinal cancer cells.
33872826	1	13	theme	cancer	352:357	arg1	cells					359:363	gastrointestinal cancer cells	335:363	gastrointestinal cancer cells	335:363	The present study aims to evaluate the synthesis of nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel and characterized its physico-chemical properties for targeted drug delivery to gastrointestinal cancer cells.
33872826	2	14	theme	UV-vis	587:592	arg1	spectrophotometry					594:610	UV-vis spectrophotometry	587:610	UV-vis spectrophotometry (UV-vis)	587:619	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	4	15	theme	cancer	1098:1103	arg1	cells					1105:1109	the gastrointestinal cancer cells	1077:1109	the gastrointestinal cancer cells	1077:1109	in vitro free radical scavenging assays confirm that the fabricated nanomaterial inhibits reactive oxygen species and generates singlet oxygen radicals in the gastrointestinal cancer cells by.
33872826	3	16	dep	in	701:702	arg1	vitro					704:708	vitro	704:708	vitro	704:708	in vitro cytotoxicity, BrdU cell proliferation, and scratch assays demonstrate that the nanohydroxyapatite/collagen-loaded fucoidan-based nanomaterial exhibits non-toxicity and increases cell proliferation and migration.
33872826	1	17	theme	fucoidan-based	221:234	arg1	hydrogel					246:253	nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel	186:253	nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel	186:253	The present study aims to evaluate the synthesis of nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel and characterized its physico-chemical properties for targeted drug delivery to gastrointestinal cancer cells.
33872826	4	18	theme	gastrointestinal	1081:1096	arg1	cells					1105:1109	the gastrointestinal cancer cells	1077:1109	the gastrointestinal cancer cells	1077:1109	in vitro free radical scavenging assays confirm that the fabricated nanomaterial inhibits reactive oxygen species and generates singlet oxygen radicals in the gastrointestinal cancer cells by.
33872826	4	19	theme	scavenging	944:953	arg1	assays					955:960	in vitro free radical scavenging assays	922:960	in vitro free radical scavenging assays	922:960	in vitro free radical scavenging assays confirm that the fabricated nanomaterial inhibits reactive oxygen species and generates singlet oxygen radicals in the gastrointestinal cancer cells by.
33872826	1	20	theme	composite	236:244	arg1	hydrogel					246:253	nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel	186:253	nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel	186:253	The present study aims to evaluate the synthesis of nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel and characterized its physico-chemical properties for targeted drug delivery to gastrointestinal cancer cells.
33872826	5	21	theme	proposed	1153:1160	arg1	carrier					1194:1200	a potential carrier	1182:1200	a potential carrier for the targeted drug delivery to the gastrointestinal cancer cells	1182:1268	The current findings suggest that the proposed nanomaterial can be a potential carrier for the targeted drug delivery to the gastrointestinal cancer cells.
33872826	5	21	theme	proposed	1153:1160	arg1	nanomaterial					1162:1173	the proposed nanomaterial	1149:1173	the proposed nanomaterial	1149:1173	The current findings suggest that the proposed nanomaterial can be a potential carrier for the targeted drug delivery to the gastrointestinal cancer cells.
33872826	2	22	theme	Thermogravimetric	553:569	arg1	TGA					581:583	TGA	581:583	TGA	581:583	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	2	22	theme	Thermogravimetric	553:569	arg1	analysis					571:578	Thermogravimetric analysis	553:578	Thermogravimetric analysis (TGA)	553:584	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	1	23	theme	hydrogel	246:253	arg1	synthesis					173:181	the synthesis	169:181	the synthesis of nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel	169:253	The present study aims to evaluate the synthesis of nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel and characterized its physico-chemical properties for targeted drug delivery to gastrointestinal cancer cells.
33872826	2	24	theme	X-ray	528:532	arg1	XRD					547:549	XRD	547:549	XRD	547:549	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	2	24	theme	X-ray	528:532	arg1	diffraction					534:544	X-ray diffraction	528:544	X-ray diffraction (XRD)	528:550	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	2	25	theme	electron	674:681	arg1	microscopy					683:692	transmission electron microscopy	661:692	transmission electron microscopy (TEM)	661:698	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	2	25	theme	electron	674:681	arg1	TEM					695:697	TEM	695:697	TEM	695:697	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	4	26	theme	singlet	1050:1056	arg1	radicals					1065:1072	singlet oxygen radicals	1050:1072	singlet oxygen radicals in the gastrointestinal cancer cells	1050:1109	in vitro free radical scavenging assays confirm that the fabricated nanomaterial inhibits reactive oxygen species and generates singlet oxygen radicals in the gastrointestinal cancer cells by.
33872826	0	27	theme	nanohydroxyapatite/collagen-loaded	13:46	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of nanohydroxyapatite/collagen-loaded	0:46	Synthesis of nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel for drug delivery to gastrointestinal cancer cells.
33872826	3	28	theme	nanohydroxyapatite/collagen-loaded	789:822	arg1	nanomaterial					839:850	the nanohydroxyapatite/collagen-loaded fucoidan-based nanomaterial	785:850	the nanohydroxyapatite/collagen-loaded fucoidan-based nanomaterial	785:850	in vitro cytotoxicity, BrdU cell proliferation, and scratch assays demonstrate that the nanohydroxyapatite/collagen-loaded fucoidan-based nanomaterial exhibits non-toxicity and increases cell proliferation and migration.
33872826	4	29	from	radicals	1065:1072	arg1	cells					1105:1109	the gastrointestinal cancer cells	1077:1109	the gastrointestinal cancer cells	1077:1109	in vitro free radical scavenging assays confirm that the fabricated nanomaterial inhibits reactive oxygen species and generates singlet oxygen radicals in the gastrointestinal cancer cells by.
33872826	3	30	theme	in	701:702	arg1	cytotoxicity					710:721	in vitro cytotoxicity	701:721	in vitro cytotoxicity	701:721	in vitro cytotoxicity, BrdU cell proliferation, and scratch assays demonstrate that the nanohydroxyapatite/collagen-loaded fucoidan-based nanomaterial exhibits non-toxicity and increases cell proliferation and migration.
33872826	1	31	theme	present	138:144	arg1	study					146:150	The present study	134:150	The present study	134:150	The present study aims to evaluate the synthesis of nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel and characterized its physico-chemical properties for targeted drug delivery to gastrointestinal cancer cells.
33872826	3	32	theme	fucoidan-based	824:837	arg1	nanomaterial					839:850	the nanohydroxyapatite/collagen-loaded fucoidan-based nanomaterial	785:850	the nanohydroxyapatite/collagen-loaded fucoidan-based nanomaterial	785:850	in vitro cytotoxicity, BrdU cell proliferation, and scratch assays demonstrate that the nanohydroxyapatite/collagen-loaded fucoidan-based nanomaterial exhibits non-toxicity and increases cell proliferation and migration.
33872826	2	33	dep	spectrophotometry	594:610	arg1	UV-vis					613:618	UV-vis	613:618	UV-vis	613:618	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	1	34	theme	physico-chemical	277:292	arg1	properties					294:303	its physico-chemical properties	273:303	its physico-chemical properties for targeted drug delivery to gastrointestinal cancer cells	273:363	The present study aims to evaluate the synthesis of nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel and characterized its physico-chemical properties for targeted drug delivery to gastrointestinal cancer cells.
33872826	0	35	theme	composite	63:71	arg1	hydrogel					73:80	composite hydrogel	63:80	composite hydrogel for drug delivery to gastrointestinal cancer cells	63:131	Synthesis of nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel for drug delivery to gastrointestinal cancer cells.
33872826	4	36	theme	oxygen	1058:1063	arg1	radicals					1065:1072	singlet oxygen radicals	1050:1072	singlet oxygen radicals in the gastrointestinal cancer cells	1050:1109	in vitro free radical scavenging assays confirm that the fabricated nanomaterial inhibits reactive oxygen species and generates singlet oxygen radicals in the gastrointestinal cancer cells by.
33872826	5	37	theme	targeted	1210:1217	arg1	delivery					1224:1231	the targeted drug delivery	1206:1231	the targeted drug delivery to the gastrointestinal cancer cells	1206:1268	The current findings suggest that the proposed nanomaterial can be a potential carrier for the targeted drug delivery to the gastrointestinal cancer cells.
33872826	2	38	dep	infrared	502:509	arg1	FTIR					512:515	FTIR	512:515	FTIR	512:515	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	2	39	theme	infrared	502:509	arg1	analysis					518:525	infrared (FTIR) analysis	502:525	infrared (FTIR) analysis	502:525	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	0	40	theme	drug	86:89	arg1	delivery					91:98	drug delivery	86:98	drug delivery to gastrointestinal cancer cells	86:131	Synthesis of nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel for drug delivery to gastrointestinal cancer cells.
33872826	4	41	theme	oxygen	1021:1026	arg1	species					1028:1034	reactive oxygen species	1012:1034	reactive oxygen species	1012:1034	in vitro free radical scavenging assays confirm that the fabricated nanomaterial inhibits reactive oxygen species and generates singlet oxygen radicals in the gastrointestinal cancer cells by.
33872826	2	42	with	nanosheets	434:443	arg1	stability					465:473	a high thermal stability	450:473	a high thermal stability	450:473	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	4	43	theme	radical	936:942	arg1	assays					955:960	in vitro free radical scavenging assays	922:960	in vitro free radical scavenging assays	922:960	in vitro free radical scavenging assays confirm that the fabricated nanomaterial inhibits reactive oxygen species and generates singlet oxygen radicals in the gastrointestinal cancer cells by.
33872826	4	44	theme	reactive	1012:1019	arg1	species					1028:1034	reactive oxygen species	1012:1034	reactive oxygen species	1012:1034	in vitro free radical scavenging assays confirm that the fabricated nanomaterial inhibits reactive oxygen species and generates singlet oxygen radicals in the gastrointestinal cancer cells by.
33872826	3	45	theme	BrdU	724:727	arg1	proliferation					734:746	BrdU cell proliferation	724:746	BrdU cell proliferation	724:746	in vitro cytotoxicity, BrdU cell proliferation, and scratch assays demonstrate that the nanohydroxyapatite/collagen-loaded fucoidan-based nanomaterial exhibits non-toxicity and increases cell proliferation and migration.
33872826	4	46	theme	free	931:934	arg1	assays					955:960	in vitro free radical scavenging assays	922:960	in vitro free radical scavenging assays	922:960	in vitro free radical scavenging assays confirm that the fabricated nanomaterial inhibits reactive oxygen species and generates singlet oxygen radicals in the gastrointestinal cancer cells by.
33872826	2	47	theme	electron	631:638	arg1	SEM					652:654	SEM	652:654	SEM	652:654	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	2	47	theme	electron	631:638	arg1	microscopy					640:649	scanning electron microscopy	622:649	scanning electron microscopy (SEM)	622:655	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	3	48	theme	cell	729:732	arg1	proliferation					734:746	BrdU cell proliferation	724:746	BrdU cell proliferation	724:746	in vitro cytotoxicity, BrdU cell proliferation, and scratch assays demonstrate that the nanohydroxyapatite/collagen-loaded fucoidan-based nanomaterial exhibits non-toxicity and increases cell proliferation and migration.
33872826	5	49	theme	current	1119:1125	arg1	findings					1127:1134	The current findings	1115:1134	The current findings	1115:1134	The current findings suggest that the proposed nanomaterial can be a potential carrier for the targeted drug delivery to the gastrointestinal cancer cells.
33872826	4	50	theme	in	922:923	arg1	assays					955:960	in vitro free radical scavenging assays	922:960	in vitro free radical scavenging assays	922:960	in vitro free radical scavenging assays confirm that the fabricated nanomaterial inhibits reactive oxygen species and generates singlet oxygen radicals in the gastrointestinal cancer cells by.
33872826	2	51	theme	spherical	424:432	arg1	nanosheets					434:443	small spherical nanosheets	418:443	small spherical nanosheets with a high thermal stability	418:473	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	2	51	theme	spherical	424:432	arg1	nanomaterial					370:381	The nanomaterial	366:381	The nanomaterial	366:381	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	2	52	theme	scanning	622:629	arg1	SEM					652:654	SEM	652:654	SEM	652:654	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	2	52	theme	scanning	622:629	arg1	microscopy					640:649	scanning electron microscopy	622:649	scanning electron microscopy (SEM)	622:655	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	3	53	theme	cytotoxicity	710:721	arg1	assays					761:766	in vitro cytotoxicity, BrdU cell proliferation, and scratch assays	701:766	in vitro cytotoxicity, BrdU cell proliferation, and scratch assays	701:766	in vitro cytotoxicity, BrdU cell proliferation, and scratch assays demonstrate that the nanohydroxyapatite/collagen-loaded fucoidan-based nanomaterial exhibits non-toxicity and increases cell proliferation and migration.
33872826	2	54	theme	small	418:422	arg1	nanosheets					434:443	small spherical nanosheets	418:443	small spherical nanosheets with a high thermal stability	418:473	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	2	54	theme	small	418:422	arg1	nanomaterial					370:381	The nanomaterial	366:381	The nanomaterial	366:381	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	1	55	theme	targeted	309:316	arg1	delivery					323:330	targeted drug delivery	309:330	targeted drug delivery to gastrointestinal cancer cells	309:363	The present study aims to evaluate the synthesis of nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel and characterized its physico-chemical properties for targeted drug delivery to gastrointestinal cancer cells.
33872826	4	56	dep	in	922:923	arg1	vitro					925:929	vitro	925:929	vitro	925:929	in vitro free radical scavenging assays confirm that the fabricated nanomaterial inhibits reactive oxygen species and generates singlet oxygen radicals in the gastrointestinal cancer cells by.
33872826	1	57	theme	drug	318:321	arg1	delivery					323:330	targeted drug delivery	309:330	targeted drug delivery to gastrointestinal cancer cells	309:363	The present study aims to evaluate the synthesis of nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel and characterized its physico-chemical properties for targeted drug delivery to gastrointestinal cancer cells.
33872826	5	58	theme	cancer	1257:1262	arg1	cells					1264:1268	the gastrointestinal cancer cells	1236:1268	the gastrointestinal cancer cells	1236:1268	The current findings suggest that the proposed nanomaterial can be a potential carrier for the targeted drug delivery to the gastrointestinal cancer cells.
33872826	2	59	dep	Fourier	484:490	arg1	transform					492:500	transform	492:500	transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM)	492:698	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
33872826	2	60	theme	thermal	457:463	arg1	stability					465:473	a high thermal stability	450:473	a high thermal stability	450:473	The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
31915902	9	0	theme	IgG	1503:1505	arg1	N-glycans					1507:1515	IgG N-glycans	1503:1515	IgG N-glycans	1503:1515	KEY POINTS: • Small molecular medium additives only affect glycosylation levels of IgG N-glycans.
31915902	11	1	theme	N-glycoforms	1608:1619	arg1	skewing					1593:1599	• BSA's skewing	1585:1599	• BSA's skewing of IgG N-glycoforms	1585:1619	• BSA's skewing of IgG N-glycoforms should be considered in IgG production.
31915902	4	2	theme	desorption/ionization	661:681	arg1	spectrometry					734:745	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry	637:745	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach	637:780	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	4	2	theme	desorption/ionization	661:681	arg1	MSn					762:764	MALDI-QIT-TOF MSn	748:764	MALDI-QIT-TOF MSn	748:764	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	4	3	from	effects	474:480	arg1	N-glycoforms					489:500	IgG N-glycoforms	485:500	IgG N-glycoforms	485:500	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	4	4	theme	laser	655:659	arg1	spectrometry					734:745	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry	637:745	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach	637:780	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	4	4	theme	laser	655:659	arg1	MSn					762:764	MALDI-QIT-TOF MSn	748:764	MALDI-QIT-TOF MSn	748:764	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	9	5	theme	glycosylation	1479:1491	arg1	levels					1493:1498	glycosylation levels	1479:1498	glycosylation levels of IgG N-glycans	1479:1515	KEY POINTS: • Small molecular medium additives only affect glycosylation levels of IgG N-glycans.
31915902	7	6	theme	IgG	1178:1180	arg1	N-glycoforms					1182:1193	IgG N-glycoforms	1178:1193	IgG N-glycoforms	1178:1193	Together, our finding suggests that BSA increases the complexity of IgG N-glycoforms, thus raising the difficulty in maintaining glycoforms consistency during antibody production.
31915902	9	7	theme	•	1432:1432	arg1	additives					1457:1465	• Small molecular medium additives	1432:1465	• Small molecular medium additives	1432:1465	KEY POINTS: • Small molecular medium additives only affect glycosylation levels of IgG N-glycans.
31915902	2	8	theme	adjusted	260:267	arg1	media					277:281	finely adjusted culture media	253:281	finely adjusted culture media	253:281	These cell lines are grown in finely adjusted culture media, which contain components that may impact glycoforms.
31915902	2	9	theme	culture	269:275	arg1	media					277:281	finely adjusted culture media	253:281	finely adjusted culture media	253:281	These cell lines are grown in finely adjusted culture media, which contain components that may impact glycoforms.
31915902	9	10	theme	KEY	1420:1422	arg1	POINTS					1424:1429	KEY POINTS	1420:1429	KEY POINTS: • Small molecular medium additives only affect glycosylation levels of IgG N-glycans.	1420:1516	KEY POINTS: • Small molecular medium additives only affect glycosylation levels of IgG N-glycans.
31915902	0	11	theme	hybridoma	90:98	arg1	supernatants					100:111	hybridoma supernatants	90:111	hybridoma supernatants	90:111	Bovine serum albumin affects N-glycoforms of murine IgG monoclonal antibody purified from hybridoma supernatants.
31915902	5	12	theme	small	796:800	arg1	additives					812:820	small molecular additives	796:820	small molecular additives	796:820	We show that small molecular additives caused little change in glycan species, though a number of these reagents, especially glutamine, affected levels of glycosylation.
31915902	8	13	theme	IgG	1322:1324	arg1	N-glycans					1326:1334	IgG N-glycans	1322:1334	IgG N-glycans	1322:1334	Therefore, the effect of BSA on IgG N-glycans should be considered when designing optimal medium formulations for IgG production.
31915902	4	14	theme	MALDI-QIT-TOF	748:760	arg1	spectrometry					734:745	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry	637:745	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach	637:780	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	4	14	theme	MALDI-QIT-TOF	748:760	arg1	MSn					762:764	MALDI-QIT-TOF MSn	748:764	MALDI-QIT-TOF MSn	748:764	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	6	15	theme	glycosylation	1088:1100	arg1	levels					1102:1107	glycosylation levels	1088:1107	glycosylation levels	1088:1107	In comparison, the addition of macromolecular protein BSA significantly changed IgG N-glycan patterns, not only in species but also in glycosylation levels.
31915902	5	16	theme	reagents	887:894	arg1	number					871:876	a number	869:876	a number of these reagents	869:894	We show that small molecular additives caused little change in glycan species, though a number of these reagents, especially glutamine, affected levels of glycosylation.
31915902	5	16	theme	reagents	887:894	arg1	glutamine					908:916	glutamine	908:916	especially glutamine	897:916	We show that small molecular additives caused little change in glycan species, though a number of these reagents, especially glutamine, affected levels of glycosylation.
31915902	6	17	theme	N-glycan	1037:1044	arg1	patterns					1046:1053	IgG N-glycan patterns	1033:1053	IgG N-glycan patterns	1033:1053	In comparison, the addition of macromolecular protein BSA significantly changed IgG N-glycan patterns, not only in species but also in glycosylation levels.
31915902	4	18	theme	-based	766:771	arg1	approach					773:780	-based approach	766:780	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach	637:780	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	7	19	gly	N-glycoforms	1182:1193	arg1	IgG					1178:1180	IgG N-glycoforms	1178:1193	IgG N-glycoforms	1178:1193	Together, our finding suggests that BSA increases the complexity of IgG N-glycoforms, thus raising the difficulty in maintaining glycoforms consistency during antibody production.
31915902	1	20	theme	monoclonal	151:160	arg1	mAbs					174:177	mAbs	174:177	mAbs	174:177	Immunoglobulin G (IgG) is a class of monoclonal antibodies (mAbs) commonly produced in mammalian cell lines.
31915902	1	20	theme	monoclonal	151:160	arg1	antibodies					162:171	monoclonal antibodies	151:171	monoclonal antibodies (mAbs) commonly produced in mammalian cell lines	151:220	Immunoglobulin G (IgG) is a class of monoclonal antibodies (mAbs) commonly produced in mammalian cell lines.
31915902	4	21	dep	spectrometry	734:745	arg1	approach					773:780	-based approach	766:780	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach	637:780	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	11	22	theme	IgG	1645:1647	arg1	production					1649:1658	IgG production	1645:1658	IgG production	1645:1658	• BSA's skewing of IgG N-glycoforms should be considered in IgG production.
31915902	9	23	theme	N-glycans	1507:1515	arg1	levels					1493:1498	glycosylation levels	1479:1498	glycosylation levels of IgG N-glycans	1479:1515	KEY POINTS: • Small molecular medium additives only affect glycosylation levels of IgG N-glycans.
31915902	4	24	theme	bovine	576:581	arg1	BSA					598:600	BSA	598:600	BSA	598:600	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	4	24	theme	bovine	576:581	arg1	albumin					589:595	bovine serum albumin	576:595	bovine serum albumin (BSA)	576:601	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	1	25	theme	antibodies	162:171	arg1	G					129:129	Immunoglobulin G	114:129	Immunoglobulin G (IgG)	114:135	Immunoglobulin G (IgG) is a class of monoclonal antibodies (mAbs) commonly produced in mammalian cell lines.
31915902	1	25	theme	antibodies	162:171	arg1	class					142:146	a class	140:146	a class of monoclonal antibodies (mAbs) commonly produced in mammalian cell lines	140:220	Immunoglobulin G (IgG) is a class of monoclonal antibodies (mAbs) commonly produced in mammalian cell lines.
31915902	3	26	theme	N-glycoforms	353:364	arg1	variation					340:348	variation	340:348	variation of N-glycoforms	340:364	As variation of N-glycoforms can impact the biological properties of IgGs, medium composition should be controlled.
31915902	11	27	gly	N-glycoforms	1608:1619	arg1	IgG					1604:1606	IgG N-glycoforms	1604:1619	IgG N-glycoforms	1604:1619	• BSA's skewing of IgG N-glycoforms should be considered in IgG production.
31915902	4	28	theme	small	614:618	arg1	molecules					620:628	other small molecules	608:628	other small molecules	608:628	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	4	29	theme	mass	729:732	arg1	spectrometry					734:745	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry	637:745	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach	637:780	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	4	29	theme	mass	729:732	arg1	MSn					762:764	MALDI-QIT-TOF MSn	748:764	MALDI-QIT-TOF MSn	748:764	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	11	30	theme	•	1585:1585	arg1	BSA					1587:1589	• BSA's	1585:1591	• BSA's skewing of IgG N-glycoforms	1585:1619	• BSA's skewing of IgG N-glycoforms should be considered in IgG production.
31915902	9	31	theme	Small	1434:1438	arg1	additives					1457:1465	• Small molecular medium additives	1432:1465	• Small molecular medium additives	1432:1465	KEY POINTS: • Small molecular medium additives only affect glycosylation levels of IgG N-glycans.
31915902	0	32	theme	Bovine	0:5	arg1	albumin					13:19	Bovine serum albumin	0:19	Bovine serum albumin	0:19	Bovine serum albumin affects N-glycoforms of murine IgG monoclonal antibody purified from hybridoma supernatants.
31915902	4	33	theme	multistage	718:727	arg1	spectrometry					734:745	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry	637:745	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach	637:780	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	4	33	theme	multistage	718:727	arg1	MSn					762:764	MALDI-QIT-TOF MSn	748:764	MALDI-QIT-TOF MSn	748:764	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	3	34	theme	IgGs	406:409	arg1	properties					392:401	the biological properties	377:401	the biological properties of IgGs	377:409	As variation of N-glycoforms can impact the biological properties of IgGs, medium composition should be controlled.
31915902	5	35	theme	molecular	802:810	arg1	additives					812:820	small molecular additives	796:820	small molecular additives	796:820	We show that small molecular additives caused little change in glycan species, though a number of these reagents, especially glutamine, affected levels of glycosylation.
31915902	4	36	theme	components	515:524	arg1	effects					474:480	the effects	470:480	the effects on IgG N-glycoforms of different components	470:524	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	8	37	from	effect	1305:1310	arg1	N-glycans					1326:1334	IgG N-glycans	1322:1334	IgG N-glycans	1322:1334	Therefore, the effect of BSA on IgG N-glycans should be considered when designing optimal medium formulations for IgG production.
31915902	8	38	theme	BSA	1315:1317	arg1	effect					1305:1310	the effect	1301:1310	the effect of BSA on IgG N-glycans	1301:1334	Therefore, the effect of BSA on IgG N-glycans should be considered when designing optimal medium formulations for IgG production.
31915902	9	39	theme	medium	1450:1455	arg1	additives					1457:1465	• Small molecular medium additives	1432:1465	• Small molecular medium additives	1432:1465	KEY POINTS: • Small molecular medium additives only affect glycosylation levels of IgG N-glycans.
31915902	10	40	gly	N-glycoforms	1550:1561	arg1	IgG					1546:1548	IgG N-glycoforms	1546:1561	IgG N-glycoforms	1546:1561	• BSA significantly changes IgG N-glycoforms as a medium additive.
31915902	4	41	theme	culture	539:545	arg1	media					547:551	hybridoma culture media	529:551	hybridoma culture media	529:551	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	4	42	theme	serum	583:587	arg1	BSA					598:600	BSA	598:600	BSA	598:600	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	4	42	theme	serum	583:587	arg1	albumin					589:595	bovine serum albumin	576:595	bovine serum albumin (BSA)	576:601	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	1	43	theme	Immunoglobulin	114:127	arg1	IgG					132:134	IgG	132:134	IgG	132:134	Immunoglobulin G (IgG) is a class of monoclonal antibodies (mAbs) commonly produced in mammalian cell lines.
31915902	1	43	theme	Immunoglobulin	114:127	arg1	G					129:129	Immunoglobulin G	114:129	Immunoglobulin G (IgG)	114:135	Immunoglobulin G (IgG) is a class of monoclonal antibodies (mAbs) commonly produced in mammalian cell lines.
31915902	1	43	theme	Immunoglobulin	114:127	arg1	class					142:146	a class	140:146	a class of monoclonal antibodies (mAbs) commonly produced in mammalian cell lines	140:220	Immunoglobulin G (IgG) is a class of monoclonal antibodies (mAbs) commonly produced in mammalian cell lines.
31915902	5	44	theme	little	829:834	arg1	change					836:841	little change	829:841	little change in glycan species	829:859	We show that small molecular additives caused little change in glycan species, though a number of these reagents, especially glutamine, affected levels of glycosylation.
31915902	3	45	theme	biological	381:390	arg1	properties					392:401	the biological properties	377:401	the biological properties of IgGs	377:409	As variation of N-glycoforms can impact the biological properties of IgGs, medium composition should be controlled.
31915902	4	46	theme	hybridoma	529:537	arg1	media					547:551	hybridoma culture media	529:551	hybridoma culture media	529:551	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	6	47	theme	IgG	1033:1035	arg1	patterns					1046:1053	IgG N-glycan patterns	1033:1053	IgG N-glycan patterns	1033:1053	In comparison, the addition of macromolecular protein BSA significantly changed IgG N-glycan patterns, not only in species but also in glycosylation levels.
31915902	9	48	theme	molecular	1440:1448	arg1	additives					1457:1465	• Small molecular medium additives	1432:1465	• Small molecular medium additives	1432:1465	KEY POINTS: • Small molecular medium additives only affect glycosylation levels of IgG N-glycans.
31915902	4	49	theme	other	608:612	arg1	molecules					620:628	other small molecules	608:628	other small molecules	608:628	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	5	50	theme	glycan	846:851	arg1	species					853:859	glycan species	846:859	glycan species	846:859	We show that small molecular additives caused little change in glycan species, though a number of these reagents, especially glutamine, affected levels of glycosylation.
31915902	8	51	theme	medium	1380:1385	arg1	formulations					1387:1398	optimal medium formulations	1372:1398	optimal medium formulations for IgG production	1372:1417	Therefore, the effect of BSA on IgG N-glycans should be considered when designing optimal medium formulations for IgG production.
31915902	5	52	from	change	836:841	arg1	species					853:859	glycan species	846:859	glycan species	846:859	We show that small molecular additives caused little change in glycan species, though a number of these reagents, especially glutamine, affected levels of glycosylation.
31915902	0	53	theme	IgG	52:54	arg1	antibody					67:74	murine IgG monoclonal antibody	45:74	murine IgG monoclonal antibody purified from hybridoma supernatants	45:111	Bovine serum albumin affects N-glycoforms of murine IgG monoclonal antibody purified from hybridoma supernatants.
31915902	4	54	theme	IgG	485:487	arg1	N-glycoforms					489:500	IgG N-glycoforms	485:500	IgG N-glycoforms	485:500	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	7	55	theme	N-glycoforms	1182:1193	arg1	complexity					1164:1173	the complexity	1160:1173	the complexity of IgG N-glycoforms	1160:1193	Together, our finding suggests that BSA increases the complexity of IgG N-glycoforms, thus raising the difficulty in maintaining glycoforms consistency during antibody production.
31915902	8	56	theme	IgG	1404:1406	arg1	production					1408:1417	IgG production	1404:1417	IgG production	1404:1417	Therefore, the effect of BSA on IgG N-glycans should be considered when designing optimal medium formulations for IgG production.
31915902	4	57	theme	ion	694:696	arg1	spectrometry					734:745	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry	637:745	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach	637:780	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	4	57	theme	ion	694:696	arg1	MSn					762:764	MALDI-QIT-TOF MSn	748:764	MALDI-QIT-TOF MSn	748:764	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	0	58	theme	murine	45:50	arg1	antibody					67:74	murine IgG monoclonal antibody	45:74	murine IgG monoclonal antibody purified from hybridoma supernatants	45:111	Bovine serum albumin affects N-glycoforms of murine IgG monoclonal antibody purified from hybridoma supernatants.
31915902	4	59	theme	different	505:513	arg1	components					515:524	different components	505:524	different components	505:524	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	10	60	theme	•	1518:1518	arg1	BSA					1520:1522	• BSA	1518:1522	• BSA	1518:1522	• BSA significantly changes IgG N-glycoforms as a medium additive.
31915902	7	61	theme	antibody	1269:1276	arg1	production					1278:1287	antibody production	1269:1287	antibody production	1269:1287	Together, our finding suggests that BSA increases the complexity of IgG N-glycoforms, thus raising the difficulty in maintaining glycoforms consistency during antibody production.
31915902	4	62	theme	quadrupole	683:692	arg1	spectrometry					734:745	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry	637:745	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach	637:780	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	4	62	theme	quadrupole	683:692	arg1	MSn					762:764	MALDI-QIT-TOF MSn	748:764	MALDI-QIT-TOF MSn	748:764	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	7	63	theme	glycoforms	1239:1248	arg1	consistency					1250:1260	glycoforms consistency	1239:1260	glycoforms consistency	1239:1260	Together, our finding suggests that BSA increases the complexity of IgG N-glycoforms, thus raising the difficulty in maintaining glycoforms consistency during antibody production.
31915902	9	64	dep	POINTS	1424:1429	arg1	affect					1472:1477	affect	1472:1477	affect glycosylation levels of IgG N-glycans	1472:1515	KEY POINTS: • Small molecular medium additives only affect glycosylation levels of IgG N-glycans.
31915902	0	65	gly	N-glycoforms	29:40	arg1	antibody					67:74	murine IgG monoclonal antibody	45:74	murine IgG monoclonal antibody purified from hybridoma supernatants	45:111	Bovine serum albumin affects N-glycoforms of murine IgG monoclonal antibody purified from hybridoma supernatants.
31915902	6	66	theme	BSA	1007:1009	arg1	addition					972:979	the addition	968:979	the addition of macromolecular protein BSA	968:1009	In comparison, the addition of macromolecular protein BSA significantly changed IgG N-glycan patterns, not only in species but also in glycosylation levels.
31915902	4	67	theme	time-of-flight	703:716	arg1	spectrometry					734:745	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry	637:745	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach	637:780	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	4	67	theme	time-of-flight	703:716	arg1	MSn					762:764	MALDI-QIT-TOF MSn	748:764	MALDI-QIT-TOF MSn	748:764	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	8	68	theme	optimal	1372:1378	arg1	formulations					1387:1398	optimal medium formulations	1372:1398	optimal medium formulations for IgG production	1372:1417	Therefore, the effect of BSA on IgG N-glycans should be considered when designing optimal medium formulations for IgG production.
31915902	1	69	theme	mammalian	201:209	arg1	lines					216:220	mammalian cell lines	201:220	mammalian cell lines	201:220	Immunoglobulin G (IgG) is a class of monoclonal antibodies (mAbs) commonly produced in mammalian cell lines.
31915902	10	70	theme	IgG	1546:1548	arg1	N-glycoforms					1550:1561	IgG N-glycoforms	1546:1561	IgG N-glycoforms	1546:1561	• BSA significantly changes IgG N-glycoforms as a medium additive.
31915902	10	70	theme	IgG	1546:1548	arg1	additive					1575:1582	additive	1575:1582	additive	1575:1582	• BSA significantly changes IgG N-glycoforms as a medium additive.
31915902	5	71	theme	glycosylation	938:950	arg1	levels					928:933	levels	928:933	levels of glycosylation	928:950	We show that small molecular additives caused little change in glycan species, though a number of these reagents, especially glutamine, affected levels of glycosylation.
31915902	11	72	theme	IgG	1604:1606	arg1	N-glycoforms					1608:1619	IgG N-glycoforms	1604:1619	IgG N-glycoforms	1604:1619	• BSA's skewing of IgG N-glycoforms should be considered in IgG production.
31915902	4	73	theme	trap	698:701	arg1	spectrometry					734:745	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry	637:745	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach	637:780	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	4	73	theme	trap	698:701	arg1	MSn					762:764	MALDI-QIT-TOF MSn	748:764	MALDI-QIT-TOF MSn	748:764	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	1	74	theme	cell	211:214	arg1	lines					216:220	mammalian cell lines	201:220	mammalian cell lines	201:220	Immunoglobulin G (IgG) is a class of monoclonal antibodies (mAbs) commonly produced in mammalian cell lines.
31915902	0	75	theme	antibody	67:74	arg1	N-glycoforms					29:40	N-glycoforms	29:40	N-glycoforms of murine IgG monoclonal antibody purified from hybridoma supernatants	29:111	Bovine serum albumin affects N-glycoforms of murine IgG monoclonal antibody purified from hybridoma supernatants.
31915902	2	76	theme	cell	229:232	arg1	lines					234:238	These cell lines	223:238	These cell lines	223:238	These cell lines are grown in finely adjusted culture media, which contain components that may impact glycoforms.
31915902	4	77	gly	N-glycoforms	489:500	arg1	IgG					485:487	IgG N-glycoforms	485:500	IgG N-glycoforms	485:500	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	4	78	theme	matrix-assisted	639:653	arg1	spectrometry					734:745	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry	637:745	a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach	637:780	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	4	78	theme	matrix-assisted	639:653	arg1	MSn					762:764	MALDI-QIT-TOF MSn	748:764	MALDI-QIT-TOF MSn	748:764	Here, we studied the effects on IgG N-glycoforms of different components in hybridoma culture media, specifically compared bovine serum albumin (BSA) with other small molecules, using a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight multistage mass spectrometry (MALDI-QIT-TOF MSn)-based approach.
31915902	3	79	theme	medium	412:417	arg1	composition					419:429	medium composition	412:429	medium composition	412:429	As variation of N-glycoforms can impact the biological properties of IgGs, medium composition should be controlled.
31915902	6	80	theme	protein	999:1005	arg1	BSA					1007:1009	macromolecular protein BSA	984:1009	macromolecular protein BSA	984:1009	In comparison, the addition of macromolecular protein BSA significantly changed IgG N-glycan patterns, not only in species but also in glycosylation levels.
31915902	0	81	theme	monoclonal	56:65	arg1	antibody					67:74	murine IgG monoclonal antibody	45:74	murine IgG monoclonal antibody purified from hybridoma supernatants	45:111	Bovine serum albumin affects N-glycoforms of murine IgG monoclonal antibody purified from hybridoma supernatants.
31915902	2	82	contain	contain	290:296	arg1	media					277:281	finely adjusted culture media	253:281	finely adjusted culture media	253:281	These cell lines are grown in finely adjusted culture media, which contain components that may impact glycoforms.
31915902	2	82	contain	contain	290:296	arg2	components					298:307	components	298:307	components that may impact glycoforms	298:334	These cell lines are grown in finely adjusted culture media, which contain components that may impact glycoforms.
31915902	0	83	theme	serum	7:11	arg1	albumin					13:19	Bovine serum albumin	0:19	Bovine serum albumin	0:19	Bovine serum albumin affects N-glycoforms of murine IgG monoclonal antibody purified from hybridoma supernatants.
31915902	6	84	theme	macromolecular	984:997	arg1	BSA					1007:1009	macromolecular protein BSA	984:1009	macromolecular protein BSA	984:1009	In comparison, the addition of macromolecular protein BSA significantly changed IgG N-glycan patterns, not only in species but also in glycosylation levels.
33039842	2	0	theme	NaOH	366:369	arg1	delignification					411:425	NaOH catalytic ethanol pretreatment assisted delignification	366:425	NaOH catalytic ethanol pretreatment assisted delignification	366:425	The results showed that NaOH catalytic ethanol pretreatment assisted delignification and the reservation of cellulose and hemicellulose.
33039842	6	1	theme	gradual	995:1001	arg1	process					1003:1009	an economical feasible and gradual process	968:1009	an economical feasible and gradual process	968:1009	This study provided an economical feasible and gradual process for the generation of glucose, which was followed by fermentation and conversion to platform chemicals.
33039842	1	2	theme	various	175:181	arg1	130-180 °C					197:206	130-180 °C	197:206	130-180 °C	197:206	The effect of NaOH catalytic ethanol pretreatment under various temperatures (130-180 °C) and time (15-90 min) on the chemical composition and enzymatic saccharification of sugarcane bagasse was investigated in this study.
33039842	1	2	theme	various	175:181	arg1	temperatures					183:194	various temperatures	175:194	various temperatures (130-180 °C)	175:207	The effect of NaOH catalytic ethanol pretreatment under various temperatures (130-180 °C) and time (15-90 min) on the chemical composition and enzymatic saccharification of sugarcane bagasse was investigated in this study.
33039842	0	3	theme	bagasse	110:116	arg1	saccharification					80:95	the enzymatic saccharification	66:95	the enzymatic saccharification of sugarcane bagasse	66:116	Sodium hydroxide catalytic ethanol pretreatment and surfactant on the enzymatic saccharification of sugarcane bagasse.
33039842	3	4	theme	substantial	541:551	arg1	yield					561:565	a substantial glucose yield	539:565	a substantial glucose yield of 91.6%	539:574	When sugarcane bagasse was pretreated at 180 °C for 30 min, a substantial glucose yield of 91.6% was obtained after hydrolysis for 72 h, representing 94.6% of glucose in pretreated residue.
33039842	1	5	theme	enzymatic	262:270	arg1	saccharification					272:287	enzymatic saccharification	262:287	enzymatic saccharification	262:287	The effect of NaOH catalytic ethanol pretreatment under various temperatures (130-180 °C) and time (15-90 min) on the chemical composition and enzymatic saccharification of sugarcane bagasse was investigated in this study.
33039842	0	6	theme	sugarcane	100:108	arg1	bagasse					110:116	sugarcane bagasse	100:116	sugarcane bagasse	100:116	Sodium hydroxide catalytic ethanol pretreatment and surfactant on the enzymatic saccharification of sugarcane bagasse.
33039842	3	7	theme	%	574:574	arg1	yield					561:565	a substantial glucose yield	539:565	a substantial glucose yield of 91.6%	539:574	When sugarcane bagasse was pretreated at 180 °C for 30 min, a substantial glucose yield of 91.6% was obtained after hydrolysis for 72 h, representing 94.6% of glucose in pretreated residue.
33039842	4	8	dep	change	727:732	arg1	the					709:711	the	709:711	the	709:711	This yield was promoted with respect to the compositional change and surface alteration of pretreated substrate.
33039842	3	9	from	%	633:633	arg1	residue					660:666	pretreated residue	649:666	pretreated residue	649:666	When sugarcane bagasse was pretreated at 180 °C for 30 min, a substantial glucose yield of 91.6% was obtained after hydrolysis for 72 h, representing 94.6% of glucose in pretreated residue.
33039842	6	10	theme	economical	971:980	arg1	process					1003:1009	an economical feasible and gradual process	968:1009	an economical feasible and gradual process	968:1009	This study provided an economical feasible and gradual process for the generation of glucose, which was followed by fermentation and conversion to platform chemicals.
33039842	5	11	theme	enzyme	819:824	arg1	usage					826:830	the enzyme usage	815:830	the enzyme usage	815:830	With the supplement of Tween 80, the enzyme usage would be saved by 50% and the enzymolysis time could be shortened to 24 h while obtaining comparable glucose yield.
33039842	3	12	theme	glucose	553:559	arg1	yield					561:565	a substantial glucose yield	539:565	a substantial glucose yield of 91.6%	539:574	When sugarcane bagasse was pretreated at 180 °C for 30 min, a substantial glucose yield of 91.6% was obtained after hydrolysis for 72 h, representing 94.6% of glucose in pretreated residue.
33039842	5	13	theme	comparable	922:931	arg1	yield					941:945	comparable glucose yield	922:945	comparable glucose yield	922:945	With the supplement of Tween 80, the enzyme usage would be saved by 50% and the enzymolysis time could be shortened to 24 h while obtaining comparable glucose yield.
33039842	2	14	theme	assisted	402:409	arg1	delignification					411:425	NaOH catalytic ethanol pretreatment assisted delignification	366:425	NaOH catalytic ethanol pretreatment assisted delignification	366:425	The results showed that NaOH catalytic ethanol pretreatment assisted delignification and the reservation of cellulose and hemicellulose.
33039842	0	15	theme	Sodium	0:5	arg1	hydroxide					7:15	Sodium hydroxide	0:15	Sodium hydroxide	0:15	Sodium hydroxide catalytic ethanol pretreatment and surfactant on the enzymatic saccharification of sugarcane bagasse.
33039842	1	16	from	effect	123:128	arg1	composition					246:256	chemical composition	237:256	chemical composition	237:256	The effect of NaOH catalytic ethanol pretreatment under various temperatures (130-180 °C) and time (15-90 min) on the chemical composition and enzymatic saccharification of sugarcane bagasse was investigated in this study.
33039842	1	16	from	effect	123:128	arg1	saccharification					272:287	enzymatic saccharification	262:287	enzymatic saccharification	262:287	The effect of NaOH catalytic ethanol pretreatment under various temperatures (130-180 °C) and time (15-90 min) on the chemical composition and enzymatic saccharification of sugarcane bagasse was investigated in this study.
33039842	5	17	theme	glucose	933:939	arg1	yield					941:945	comparable glucose yield	922:945	comparable glucose yield	922:945	With the supplement of Tween 80, the enzyme usage would be saved by 50% and the enzymolysis time could be shortened to 24 h while obtaining comparable glucose yield.
33039842	2	18	theme	pretreatment	389:400	arg1	delignification					411:425	NaOH catalytic ethanol pretreatment assisted delignification	366:425	NaOH catalytic ethanol pretreatment assisted delignification	366:425	The results showed that NaOH catalytic ethanol pretreatment assisted delignification and the reservation of cellulose and hemicellulose.
33039842	0	19	theme	ethanol	27:33	arg1	pretreatment					35:46	ethanol pretreatment	27:46	ethanol pretreatment	27:46	Sodium hydroxide catalytic ethanol pretreatment and surfactant on the enzymatic saccharification of sugarcane bagasse.
33039842	1	20	theme	sugarcane	292:300	arg1	bagasse					302:308	sugarcane bagasse	292:308	sugarcane bagasse	292:308	The effect of NaOH catalytic ethanol pretreatment under various temperatures (130-180 °C) and time (15-90 min) on the chemical composition and enzymatic saccharification of sugarcane bagasse was investigated in this study.
33039842	2	21	dep	showed	354:359	arg1	delignification					411:425	NaOH catalytic ethanol pretreatment assisted delignification	366:425	NaOH catalytic ethanol pretreatment assisted delignification	366:425	The results showed that NaOH catalytic ethanol pretreatment assisted delignification and the reservation of cellulose and hemicellulose.
33039842	2	21	dep	showed	354:359	arg1	reservation					435:445	the reservation	431:445	the reservation of cellulose and hemicellulose	431:476	The results showed that NaOH catalytic ethanol pretreatment assisted delignification and the reservation of cellulose and hemicellulose.
33039842	2	22	theme	cellulose	450:458	arg1	delignification					411:425	NaOH catalytic ethanol pretreatment assisted delignification	366:425	NaOH catalytic ethanol pretreatment assisted delignification	366:425	The results showed that NaOH catalytic ethanol pretreatment assisted delignification and the reservation of cellulose and hemicellulose.
33039842	2	22	theme	cellulose	450:458	arg1	reservation					435:445	the reservation	431:445	the reservation of cellulose and hemicellulose	431:476	The results showed that NaOH catalytic ethanol pretreatment assisted delignification and the reservation of cellulose and hemicellulose.
33039842	1	23	theme	bagasse	302:308	arg1	composition					246:256	chemical composition	237:256	chemical composition	237:256	The effect of NaOH catalytic ethanol pretreatment under various temperatures (130-180 °C) and time (15-90 min) on the chemical composition and enzymatic saccharification of sugarcane bagasse was investigated in this study.
33039842	1	23	theme	bagasse	302:308	arg1	saccharification					272:287	enzymatic saccharification	262:287	enzymatic saccharification	262:287	The effect of NaOH catalytic ethanol pretreatment under various temperatures (130-180 °C) and time (15-90 min) on the chemical composition and enzymatic saccharification of sugarcane bagasse was investigated in this study.
33039842	4	24	theme	pretreated	760:769	arg1	substrate					771:779	pretreated substrate	760:779	pretreated substrate	760:779	This yield was promoted with respect to the compositional change and surface alteration of pretreated substrate.
33039842	1	25	theme	ethanol	148:154	arg1	pretreatment					156:167	NaOH catalytic ethanol pretreatment	133:167	NaOH catalytic ethanol pretreatment under various temperatures (130-180 °C) and time (15-90 min)	133:228	The effect of NaOH catalytic ethanol pretreatment under various temperatures (130-180 °C) and time (15-90 min) on the chemical composition and enzymatic saccharification of sugarcane bagasse was investigated in this study.
33039842	6	26	theme	glucose	1033:1039	arg1	generation					1019:1028	the generation	1015:1028	the generation of glucose	1015:1039	This study provided an economical feasible and gradual process for the generation of glucose, which was followed by fermentation and conversion to platform chemicals.
33039842	5	27	theme	enzymolysis	862:872	arg1	time					874:877	the enzymolysis time	858:877	the enzymolysis time	858:877	With the supplement of Tween 80, the enzyme usage would be saved by 50% and the enzymolysis time could be shortened to 24 h while obtaining comparable glucose yield.
33039842	3	28	theme	sugarcane	484:492	arg1	bagasse					494:500	sugarcane bagasse	484:500	sugarcane bagasse	484:500	When sugarcane bagasse was pretreated at 180 °C for 30 min, a substantial glucose yield of 91.6% was obtained after hydrolysis for 72 h, representing 94.6% of glucose in pretreated residue.
33039842	5	29	theme	Tween	805:809	arg1	supplement					791:800	the supplement	787:800	the supplement of Tween 80	787:812	With the supplement of Tween 80, the enzyme usage would be saved by 50% and the enzymolysis time could be shortened to 24 h while obtaining comparable glucose yield.
33039842	2	30	theme	hemicellulose	464:476	arg1	delignification					411:425	NaOH catalytic ethanol pretreatment assisted delignification	366:425	NaOH catalytic ethanol pretreatment assisted delignification	366:425	The results showed that NaOH catalytic ethanol pretreatment assisted delignification and the reservation of cellulose and hemicellulose.
33039842	2	30	theme	hemicellulose	464:476	arg1	reservation					435:445	the reservation	431:445	the reservation of cellulose and hemicellulose	431:476	The results showed that NaOH catalytic ethanol pretreatment assisted delignification and the reservation of cellulose and hemicellulose.
33039842	3	31	theme	pretreated	649:658	arg1	residue					660:666	pretreated residue	649:666	pretreated residue	649:666	When sugarcane bagasse was pretreated at 180 °C for 30 min, a substantial glucose yield of 91.6% was obtained after hydrolysis for 72 h, representing 94.6% of glucose in pretreated residue.
33039842	6	32	theme	platform	1095:1102	arg1	chemicals					1104:1112	platform chemicals	1095:1112	platform chemicals	1095:1112	This study provided an economical feasible and gradual process for the generation of glucose, which was followed by fermentation and conversion to platform chemicals.
33039842	1	33	theme	pretreatment	156:167	arg1	effect					123:128	The effect	119:128	The effect of NaOH catalytic ethanol pretreatment under various temperatures (130-180 °C) and time (15-90 min) on the chemical composition and enzymatic saccharification of sugarcane bagasse	119:308	The effect of NaOH catalytic ethanol pretreatment under various temperatures (130-180 °C) and time (15-90 min) on the chemical composition and enzymatic saccharification of sugarcane bagasse was investigated in this study.
33039842	4	34	theme	compositional	713:725	arg1	change					727:732	compositional change	713:732	compositional change	713:732	This yield was promoted with respect to the compositional change and surface alteration of pretreated substrate.
33039842	1	35	theme	NaOH	133:136	arg1	pretreatment					156:167	NaOH catalytic ethanol pretreatment	133:167	NaOH catalytic ethanol pretreatment under various temperatures (130-180 °C) and time (15-90 min)	133:228	The effect of NaOH catalytic ethanol pretreatment under various temperatures (130-180 °C) and time (15-90 min) on the chemical composition and enzymatic saccharification of sugarcane bagasse was investigated in this study.
33039842	4	36	theme	substrate	771:779	arg1	change					727:732	compositional change	713:732	compositional change	713:732	This yield was promoted with respect to the compositional change and surface alteration of pretreated substrate.
33039842	4	36	theme	substrate	771:779	arg1	alteration					746:755	surface alteration	738:755	surface alteration	738:755	This yield was promoted with respect to the compositional change and surface alteration of pretreated substrate.
33039842	1	37	dep	composition	246:256	arg1	the					233:235	the	233:235	the	233:235	The effect of NaOH catalytic ethanol pretreatment under various temperatures (130-180 °C) and time (15-90 min) on the chemical composition and enzymatic saccharification of sugarcane bagasse was investigated in this study.
33039842	3	38	theme	glucose	638:644	arg1	%					633:633	94.6%	629:633	94.6% of glucose in pretreated residue	629:666	When sugarcane bagasse was pretreated at 180 °C for 30 min, a substantial glucose yield of 91.6% was obtained after hydrolysis for 72 h, representing 94.6% of glucose in pretreated residue.
33039842	3	38	theme	glucose	638:644	arg1	glucose					638:644	glucose	638:644	glucose	638:644	When sugarcane bagasse was pretreated at 180 °C for 30 min, a substantial glucose yield of 91.6% was obtained after hydrolysis for 72 h, representing 94.6% of glucose in pretreated residue.
33039842	1	39	theme	catalytic	138:146	arg1	pretreatment					156:167	NaOH catalytic ethanol pretreatment	133:167	NaOH catalytic ethanol pretreatment under various temperatures (130-180 °C) and time (15-90 min)	133:228	The effect of NaOH catalytic ethanol pretreatment under various temperatures (130-180 °C) and time (15-90 min) on the chemical composition and enzymatic saccharification of sugarcane bagasse was investigated in this study.
33039842	4	40	theme	surface	738:744	arg1	alteration					746:755	surface alteration	738:755	surface alteration	738:755	This yield was promoted with respect to the compositional change and surface alteration of pretreated substrate.
33039842	0	41	theme	enzymatic	70:78	arg1	saccharification					80:95	the enzymatic saccharification	66:95	the enzymatic saccharification of sugarcane bagasse	66:116	Sodium hydroxide catalytic ethanol pretreatment and surfactant on the enzymatic saccharification of sugarcane bagasse.
33039842	2	42	theme	ethanol	381:387	arg1	delignification					411:425	NaOH catalytic ethanol pretreatment assisted delignification	366:425	NaOH catalytic ethanol pretreatment assisted delignification	366:425	The results showed that NaOH catalytic ethanol pretreatment assisted delignification and the reservation of cellulose and hemicellulose.
33039842	6	43	theme	feasible	982:989	arg1	process					1003:1009	an economical feasible and gradual process	968:1009	an economical feasible and gradual process	968:1009	This study provided an economical feasible and gradual process for the generation of glucose, which was followed by fermentation and conversion to platform chemicals.
33039842	2	44	theme	catalytic	371:379	arg1	delignification					411:425	NaOH catalytic ethanol pretreatment assisted delignification	366:425	NaOH catalytic ethanol pretreatment assisted delignification	366:425	The results showed that NaOH catalytic ethanol pretreatment assisted delignification and the reservation of cellulose and hemicellulose.
33039842	1	45	theme	chemical	237:244	arg1	composition					246:256	chemical composition	237:256	chemical composition	237:256	The effect of NaOH catalytic ethanol pretreatment under various temperatures (130-180 °C) and time (15-90 min) on the chemical composition and enzymatic saccharification of sugarcane bagasse was investigated in this study.
33205576	3	0	theme	cationized	514:523	arg1	esters					535:540	The resultant cationized cellulose esters	500:540	The resultant cationized cellulose esters	500:540	The resultant cationized cellulose esters effectively lock various ionic liquids (ILs) via electrostatic interactions.
33205576	8	1	contain	have	1422:1425	arg2	potential					1434:1442	a huge potential	1427:1442	a huge potential	1427:1442	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property have a huge potential in the practical utilization for gas separation.
33205576	8	1	contain	have	1422:1425	arg1	membranes					1336:1344	Such high-performance CO2 separation membranes	1299:1344	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property	1299:1420	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property have a huge potential in the practical utilization for gas separation.
33205576	3	2	theme	ionic	567:571	arg1	ILs					582:584	ILs	582:584	ILs	582:584	The resultant cationized cellulose esters effectively lock various ionic liquids (ILs) via electrostatic interactions.
33205576	3	2	theme	ionic	567:571	arg1	liquids					573:579	various ionic liquids	559:579	various ionic liquids (ILs)	559:585	The resultant cationized cellulose esters effectively lock various ionic liquids (ILs) via electrostatic interactions.
33205576	4	3	theme	cellulose	675:683	arg1	uniform					719:725	uniform	719:725	uniform	719:725	Due to the strong attraction interactions, the obtained cellulose ester/ILs composite membranes are uniform, smooth, and highly transparent.
33205576	4	3	theme	cellulose	675:683	arg1	membranes					705:713	the obtained cellulose ester/ILs composite membranes	662:713	the obtained cellulose ester/ILs composite membranes	662:713	Due to the strong attraction interactions, the obtained cellulose ester/ILs composite membranes are uniform, smooth, and highly transparent.
33205576	4	3	theme	cellulose	675:683	arg1	transparent					747:757	transparent	747:757	transparent	747:757	Due to the strong attraction interactions, the obtained cellulose ester/ILs composite membranes are uniform, smooth, and highly transparent.
33205576	4	4	theme	composite	695:703	arg1	uniform					719:725	uniform	719:725	uniform	719:725	Due to the strong attraction interactions, the obtained cellulose ester/ILs composite membranes are uniform, smooth, and highly transparent.
33205576	4	4	theme	composite	695:703	arg1	membranes					705:713	the obtained cellulose ester/ILs composite membranes	662:713	the obtained cellulose ester/ILs composite membranes	662:713	Due to the strong attraction interactions, the obtained cellulose ester/ILs composite membranes are uniform, smooth, and highly transparent.
33205576	4	4	theme	composite	695:703	arg1	transparent					747:757	transparent	747:757	transparent	747:757	Due to the strong attraction interactions, the obtained cellulose ester/ILs composite membranes are uniform, smooth, and highly transparent.
33205576	2	5	theme	imidazolium	303:313	arg1	cation					315:320	imidazolium cation	303:320	imidazolium cation	303:320	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	2	6	theme	esters	285:290	arg1	kinds					262:266	two kinds	258:266	two kinds of new cellulose esters containing imidazolium cation	258:320	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	2	6	theme	esters	285:290	arg1	imide					448:452	CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide	387:452	CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N)	387:467	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	2	6	theme	esters	285:290	arg1	chloride					374:381	cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride	323:381	cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride	323:381	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	5	7	theme	bis	828:830	arg1	anion					864:868	a bis(trifluoromethane sulfonyl)imide anion	826:868	a bis(trifluoromethane sulfonyl)imide anion	826:868	Moreover, the added ILs with a long alkyl chain in the cation and a bis(trifluoromethane sulfonyl)imide anion remarkably improve the CO2 permeability of the cellulose ester/ILs membranes, because of the dramatic increase of the CO2 diffusion rate.
33205576	5	8	theme	trifluoromethane	832:847	arg1	bis					828:830	a bis	826:830	a bis(trifluoromethane sulfonyl)imide anion	826:868	Moreover, the added ILs with a long alkyl chain in the cation and a bis(trifluoromethane sulfonyl)imide anion remarkably improve the CO2 permeability of the cellulose ester/ILs membranes, because of the dramatic increase of the CO2 diffusion rate.
33205576	5	8	theme	trifluoromethane	832:847	arg1	sulfonyl					849:856	trifluoromethane sulfonyl	832:856	trifluoromethane sulfonyl	832:856	Moreover, the added ILs with a long alkyl chain in the cation and a bis(trifluoromethane sulfonyl)imide anion remarkably improve the CO2 permeability of the cellulose ester/ILs membranes, because of the dramatic increase of the CO2 diffusion rate.
33205576	5	9	from	ILs	780:782	arg1	cation					815:820	the cation	811:820	the cation	811:820	Moreover, the added ILs with a long alkyl chain in the cation and a bis(trifluoromethane sulfonyl)imide anion remarkably improve the CO2 permeability of the cellulose ester/ILs membranes, because of the dramatic increase of the CO2 diffusion rate.
33205576	5	9	from	ILs	780:782	arg1	anion					864:868	a bis(trifluoromethane sulfonyl)imide anion	826:868	a bis(trifluoromethane sulfonyl)imide anion	826:868	Moreover, the added ILs with a long alkyl chain in the cation and a bis(trifluoromethane sulfonyl)imide anion remarkably improve the CO2 permeability of the cellulose ester/ILs membranes, because of the dramatic increase of the CO2 diffusion rate.
33205576	3	10	theme	electrostatic	591:603	arg1	interactions					605:616	electrostatic interactions	591:616	electrostatic interactions	591:616	The resultant cationized cellulose esters effectively lock various ionic liquids (ILs) via electrostatic interactions.
33205576	6	11	theme	CA-BmimTf2	1163:1172	arg1	membrane					1176:1183	CA-BmimTf2 N membrane	1163:1183	CA-BmimTf2 N membrane	1163:1183	The CA-BmimTf2 N/C10 mimTf2 N membranes exhibit the highest CO2 permeability, which is 3800% higher than that of CA membrane and 1700% higher than that of CA-BmimTf2 N membrane.
33205576	5	12	with	ILs	780:782	arg1	chain					802:806	a long alkyl chain	789:806	a long alkyl chain	789:806	Moreover, the added ILs with a long alkyl chain in the cation and a bis(trifluoromethane sulfonyl)imide anion remarkably improve the CO2 permeability of the cellulose ester/ILs membranes, because of the dramatic increase of the CO2 diffusion rate.
33205576	3	13	theme	various	559:565	arg1	ILs					582:584	ILs	582:584	ILs	582:584	The resultant cationized cellulose esters effectively lock various ionic liquids (ILs) via electrostatic interactions.
33205576	3	13	theme	various	559:565	arg1	liquids					573:579	various ionic liquids	559:579	various ionic liquids (ILs)	559:585	The resultant cationized cellulose esters effectively lock various ionic liquids (ILs) via electrostatic interactions.
33205576	5	14	theme	cellulose	917:925	arg1	membranes					937:945	the cellulose ester/ILs membranes	913:945	the cellulose ester/ILs membranes	913:945	Moreover, the added ILs with a long alkyl chain in the cation and a bis(trifluoromethane sulfonyl)imide anion remarkably improve the CO2 permeability of the cellulose ester/ILs membranes, because of the dramatic increase of the CO2 diffusion rate.
33205576	5	15	theme	added	774:778	arg1	ILs					780:782	the added ILs	770:782	the added ILs with a long alkyl chain in the cation and a bis(trifluoromethane sulfonyl)imide anion	770:868	Moreover, the added ILs with a long alkyl chain in the cation and a bis(trifluoromethane sulfonyl)imide anion remarkably improve the CO2 permeability of the cellulose ester/ILs membranes, because of the dramatic increase of the CO2 diffusion rate.
33205576	0	16	theme	Imidazolium	70:80	arg1	Cation					82:87	a New Cellulose Ester Containing Imidazolium Cation	37:87	a New Cellulose Ester Containing Imidazolium Cation for High-Performance CO2 Separation Membranes	37:133	Immobilization of Ionic Liquids with a New Cellulose Ester Containing Imidazolium Cation for High-Performance CO2 Separation Membranes.
33205576	1	17	theme	significant	161:171	arg1	importance					173:182	significant importance	161:182	significant importance	161:182	CO2 gas separation is of significant importance to protect the environment and utilize the carbon resource.
33205576	8	18	theme	gas	1477:1479	arg1	separation					1481:1490	gas separation	1477:1490	gas separation	1477:1490	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property have a huge potential in the practical utilization for gas separation.
33205576	8	19	theme	high-performance	1304:1319	arg1	membranes					1336:1344	Such high-performance CO2 separation membranes	1299:1344	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property	1299:1420	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property have a huge potential in the practical utilization for gas separation.
33205576	2	20	theme	new	271:273	arg1	esters					285:290	new cellulose esters	271:290	new cellulose esters containing imidazolium cation	271:320	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	4	21	dep	uniform	719:725	arg1	uniform					719:725	uniform	719:725	uniform	719:725	Due to the strong attraction interactions, the obtained cellulose ester/ILs composite membranes are uniform, smooth, and highly transparent.
33205576	4	21	dep	uniform	719:725	arg1	transparent					747:757	transparent	747:757	transparent	747:757	Due to the strong attraction interactions, the obtained cellulose ester/ILs composite membranes are uniform, smooth, and highly transparent.
33205576	4	21	dep	uniform	719:725	arg1	membranes					705:713	the obtained cellulose ester/ILs composite membranes	662:713	the obtained cellulose ester/ILs composite membranes	662:713	Due to the strong attraction interactions, the obtained cellulose ester/ILs composite membranes are uniform, smooth, and highly transparent.
33205576	1	22	theme	gas	140:142	arg1	separation					144:153	CO2 gas separation	136:153	CO2 gas separation	136:153	CO2 gas separation is of significant importance to protect the environment and utilize the carbon resource.
33205576	8	23	theme	separation	1325:1334	arg1	membranes					1336:1344	Such high-performance CO2 separation membranes	1299:1344	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property	1299:1420	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property have a huge potential in the practical utilization for gas separation.
33205576	2	24	theme	bis	418:420	arg1	imide					448:452	CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide	387:452	CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N)	387:467	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	2	24	theme	bis	418:420	arg1	N					466:466	CA-BmimTf2 N	455:466	CA-BmimTf2 N	455:466	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	2	24	theme	bis	418:420	arg1	kinds					262:266	two kinds	258:266	two kinds of new cellulose esters containing imidazolium cation	258:320	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	0	25	theme	CO2	110:112	arg1	Membranes					125:133	High-Performance CO2 Separation Membranes	93:133	High-Performance CO2 Separation Membranes	93:133	Immobilization of Ionic Liquids with a New Cellulose Ester Containing Imidazolium Cation for High-Performance CO2 Separation Membranes.
33205576	6	26	theme	highest	1060:1066	arg1	permeability					1072:1083	the highest CO2 permeability	1056:1083	the highest CO2 permeability	1056:1083	The CA-BmimTf2 N/C10 mimTf2 N membranes exhibit the highest CO2 permeability, which is 3800% higher than that of CA membrane and 1700% higher than that of CA-BmimTf2 N membrane.
33205576	2	27	theme	CA	387:388	arg1	imide					448:452	CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide	387:452	CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N)	387:467	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	2	27	theme	CA	387:388	arg1	N					466:466	CA-BmimTf2 N	455:466	CA-BmimTf2 N	455:466	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	2	27	theme	CA	387:388	arg1	kinds					262:266	two kinds	258:266	two kinds of new cellulose esters containing imidazolium cation	258:320	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	5	28	theme	membranes	937:945	arg1	permeability					897:908	the CO2 permeability	889:908	the CO2 permeability of the cellulose ester/ILs membranes	889:945	Moreover, the added ILs with a long alkyl chain in the cation and a bis(trifluoromethane sulfonyl)imide anion remarkably improve the CO2 permeability of the cellulose ester/ILs membranes, because of the dramatic increase of the CO2 diffusion rate.
33205576	7	29	theme	thermal	1280:1286	arg1	stability					1288:1296	thermal stability	1280:1296	thermal stability	1280:1296	More importantly, the CA-BmimTf2 N/C10 mimTf2 N membranes have good mechanical properties and thermal stability.
33205576	8	30	theme	CO2	1356:1358	arg1	permeability					1360:1371	high CO2 permeability	1351:1371	high CO2 permeability	1351:1371	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property have a huge potential in the practical utilization for gas separation.
33205576	0	31	theme	Ionic	18:22	arg1	Liquids					24:30	Ionic Liquids	18:30	Ionic Liquids	18:30	Immobilization of Ionic Liquids with a New Cellulose Ester Containing Imidazolium Cation for High-Performance CO2 Separation Membranes.
33205576	6	32	theme	N	1036:1036	arg1	membranes					1038:1046	The CA-BmimTf2 N/C10 mimTf2 N membranes	1008:1046	The CA-BmimTf2 N/C10 mimTf2 N membranes	1008:1046	The CA-BmimTf2 N/C10 mimTf2 N membranes exhibit the highest CO2 permeability, which is 3800% higher than that of CA membrane and 1700% higher than that of CA-BmimTf2 N membrane.
33205576	2	33	theme	trifluoromethane	422:437	arg1	imide					448:452	CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide	387:452	CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N)	387:467	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	2	33	theme	trifluoromethane	422:437	arg1	N					466:466	CA-BmimTf2 N	455:466	CA-BmimTf2 N	455:466	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	2	33	theme	trifluoromethane	422:437	arg1	kinds					262:266	two kinds	258:266	two kinds of new cellulose esters containing imidazolium cation	258:320	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	5	34	theme	long	791:794	arg1	chain					802:806	a long alkyl chain	789:806	a long alkyl chain	789:806	Moreover, the added ILs with a long alkyl chain in the cation and a bis(trifluoromethane sulfonyl)imide anion remarkably improve the CO2 permeability of the cellulose ester/ILs membranes, because of the dramatic increase of the CO2 diffusion rate.
33205576	7	35	theme	good	1249:1252	arg1	properties					1265:1274	good mechanical properties	1249:1274	good mechanical properties	1249:1274	More importantly, the CA-BmimTf2 N/C10 mimTf2 N membranes have good mechanical properties and thermal stability.
33205576	0	36	theme	Ester	53:57	arg1	Cation					82:87	a New Cellulose Ester Containing Imidazolium Cation	37:87	a New Cellulose Ester Containing Imidazolium Cation for High-Performance CO2 Separation Membranes	37:133	Immobilization of Ionic Liquids with a New Cellulose Ester Containing Imidazolium Cation for High-Performance CO2 Separation Membranes.
33205576	0	37	theme	New	39:41	arg1	Cation					82:87	a New Cellulose Ester Containing Imidazolium Cation	37:87	a New Cellulose Ester Containing Imidazolium Cation for High-Performance CO2 Separation Membranes	37:133	Immobilization of Ionic Liquids with a New Cellulose Ester Containing Imidazolium Cation for High-Performance CO2 Separation Membranes.
33205576	0	38	with	Immobilization	0:13	arg1	Cation					82:87	a New Cellulose Ester Containing Imidazolium Cation	37:87	a New Cellulose Ester Containing Imidazolium Cation for High-Performance CO2 Separation Membranes	37:133	Immobilization of Ionic Liquids with a New Cellulose Ester Containing Imidazolium Cation for High-Performance CO2 Separation Membranes.
33205576	2	39	theme	cellulose	323:331	arg1	acetate					333:339	cellulose acetate	323:339	cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride	323:381	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	2	39	theme	cellulose	323:331	arg1	CA					342:343	CA	342:343	CA	342:343	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	1	40	theme	carbon	227:232	arg1	resource					234:241	the carbon resource	223:241	the carbon resource	223:241	CO2 gas separation is of significant importance to protect the environment and utilize the carbon resource.
33205576	7	41	theme	mimTf2	1225:1230	arg1	membranes					1234:1242	the CA-BmimTf2 N/C10 mimTf2 N membranes	1204:1242	the CA-BmimTf2 N/C10 mimTf2 N membranes	1204:1242	More importantly, the CA-BmimTf2 N/C10 mimTf2 N membranes have good mechanical properties and thermal stability.
33205576	6	42	theme	N/C10	1023:1027	arg1	membranes					1038:1046	The CA-BmimTf2 N/C10 mimTf2 N membranes	1008:1046	The CA-BmimTf2 N/C10 mimTf2 N membranes	1008:1046	The CA-BmimTf2 N/C10 mimTf2 N membranes exhibit the highest CO2 permeability, which is 3800% higher than that of CA membrane and 1700% higher than that of CA-BmimTf2 N membrane.
33205576	8	43	theme	mechanical	1402:1411	arg1	property					1413:1420	good mechanical property	1397:1420	good mechanical property	1397:1420	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property have a huge potential in the practical utilization for gas separation.
33205576	3	44	theme	cellulose	525:533	arg1	esters					535:540	The resultant cationized cellulose esters	500:540	The resultant cationized cellulose esters	500:540	The resultant cationized cellulose esters effectively lock various ionic liquids (ILs) via electrostatic interactions.
33205576	0	45	theme	Cellulose	43:51	arg1	Cation					82:87	a New Cellulose Ester Containing Imidazolium Cation	37:87	a New Cellulose Ester Containing Imidazolium Cation for High-Performance CO2 Separation Membranes	37:133	Immobilization of Ionic Liquids with a New Cellulose Ester Containing Imidazolium Cation for High-Performance CO2 Separation Membranes.
33205576	5	46	theme	diffusion	992:1000	arg1	rate					1002:1005	the CO2 diffusion rate	984:1005	the CO2 diffusion rate	984:1005	Moreover, the added ILs with a long alkyl chain in the cation and a bis(trifluoromethane sulfonyl)imide anion remarkably improve the CO2 permeability of the cellulose ester/ILs membranes, because of the dramatic increase of the CO2 diffusion rate.
33205576	7	47	theme	CA-BmimTf2	1208:1217	arg1	membranes					1234:1242	the CA-BmimTf2 N/C10 mimTf2 N membranes	1204:1242	the CA-BmimTf2 N/C10 mimTf2 N membranes	1204:1242	More importantly, the CA-BmimTf2 N/C10 mimTf2 N membranes have good mechanical properties and thermal stability.
33205576	3	48	theme	resultant	504:512	arg1	esters					535:540	The resultant cationized cellulose esters	500:540	The resultant cationized cellulose esters	500:540	The resultant cationized cellulose esters effectively lock various ionic liquids (ILs) via electrostatic interactions.
33205576	2	49	theme	cellulose	275:283	arg1	esters					285:290	new cellulose esters	271:290	new cellulose esters containing imidazolium cation	271:320	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	4	50	theme	obtained	666:673	arg1	uniform					719:725	uniform	719:725	uniform	719:725	Due to the strong attraction interactions, the obtained cellulose ester/ILs composite membranes are uniform, smooth, and highly transparent.
33205576	4	50	theme	obtained	666:673	arg1	membranes					705:713	the obtained cellulose ester/ILs composite membranes	662:713	the obtained cellulose ester/ILs composite membranes	662:713	Due to the strong attraction interactions, the obtained cellulose ester/ILs composite membranes are uniform, smooth, and highly transparent.
33205576	4	50	theme	obtained	666:673	arg1	transparent					747:757	transparent	747:757	transparent	747:757	Due to the strong attraction interactions, the obtained cellulose ester/ILs composite membranes are uniform, smooth, and highly transparent.
33205576	5	51	theme	imide	858:862	arg1	anion					864:868	a bis(trifluoromethane sulfonyl)imide anion	826:868	a bis(trifluoromethane sulfonyl)imide anion	826:868	Moreover, the added ILs with a long alkyl chain in the cation and a bis(trifluoromethane sulfonyl)imide anion remarkably improve the CO2 permeability of the cellulose ester/ILs membranes, because of the dramatic increase of the CO2 diffusion rate.
33205576	6	52	theme	CA	1121:1122	arg1	membrane					1124:1131	CA membrane	1121:1131	CA membrane	1121:1131	The CA-BmimTf2 N/C10 mimTf2 N membranes exhibit the highest CO2 permeability, which is 3800% higher than that of CA membrane and 1700% higher than that of CA-BmimTf2 N membrane.
33205576	4	53	theme	ester/ILs	685:693	arg1	uniform					719:725	uniform	719:725	uniform	719:725	Due to the strong attraction interactions, the obtained cellulose ester/ILs composite membranes are uniform, smooth, and highly transparent.
33205576	4	53	theme	ester/ILs	685:693	arg1	membranes					705:713	the obtained cellulose ester/ILs composite membranes	662:713	the obtained cellulose ester/ILs composite membranes	662:713	Due to the strong attraction interactions, the obtained cellulose ester/ILs composite membranes are uniform, smooth, and highly transparent.
33205576	4	53	theme	ester/ILs	685:693	arg1	transparent					747:757	transparent	747:757	transparent	747:757	Due to the strong attraction interactions, the obtained cellulose ester/ILs composite membranes are uniform, smooth, and highly transparent.
33205576	5	54	theme	CO2	893:895	arg1	permeability					897:908	the CO2 permeability	889:908	the CO2 permeability of the cellulose ester/ILs membranes	889:945	Moreover, the added ILs with a long alkyl chain in the cation and a bis(trifluoromethane sulfonyl)imide anion remarkably improve the CO2 permeability of the cellulose ester/ILs membranes, because of the dramatic increase of the CO2 diffusion rate.
33205576	4	55	theme	attraction	637:646	arg1	interactions					648:659	the strong attraction interactions	626:659	the strong attraction interactions	626:659	Due to the strong attraction interactions, the obtained cellulose ester/ILs composite membranes are uniform, smooth, and highly transparent.
33205576	7	56	contain	have	1244:1247	arg2	stability					1288:1296	thermal stability	1280:1296	thermal stability	1280:1296	More importantly, the CA-BmimTf2 N/C10 mimTf2 N membranes have good mechanical properties and thermal stability.
33205576	7	56	contain	have	1244:1247	arg2	properties					1265:1274	good mechanical properties	1249:1274	good mechanical properties	1249:1274	More importantly, the CA-BmimTf2 N/C10 mimTf2 N membranes have good mechanical properties and thermal stability.
33205576	7	56	contain	have	1244:1247	arg1	membranes					1234:1242	the CA-BmimTf2 N/C10 mimTf2 N membranes	1204:1242	the CA-BmimTf2 N/C10 mimTf2 N membranes	1204:1242	More importantly, the CA-BmimTf2 N/C10 mimTf2 N membranes have good mechanical properties and thermal stability.
33205576	8	57	theme	huge	1429:1432	arg1	potential					1434:1442	a huge potential	1427:1442	a huge potential	1427:1442	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property have a huge potential in the practical utilization for gas separation.
33205576	6	58	theme	N	1174:1174	arg1	membrane					1176:1183	CA-BmimTf2 N membrane	1163:1183	CA-BmimTf2 N membrane	1163:1183	The CA-BmimTf2 N/C10 mimTf2 N membranes exhibit the highest CO2 permeability, which is 3800% higher than that of CA membrane and 1700% higher than that of CA-BmimTf2 N membrane.
33205576	1	59	theme	CO2	136:138	arg1	separation					144:153	CO2 gas separation	136:153	CO2 gas separation	136:153	CO2 gas separation is of significant importance to protect the environment and utilize the carbon resource.
33205576	6	60	theme	CA-BmimTf2	1012:1021	arg1	membranes					1038:1046	The CA-BmimTf2 N/C10 mimTf2 N membranes	1008:1046	The CA-BmimTf2 N/C10 mimTf2 N membranes	1008:1046	The CA-BmimTf2 N/C10 mimTf2 N membranes exhibit the highest CO2 permeability, which is 3800% higher than that of CA membrane and 1700% higher than that of CA-BmimTf2 N membrane.
33205576	8	61	theme	practical	1451:1459	arg1	utilization					1461:1471	the practical utilization	1447:1471	the practical utilization for gas separation	1447:1490	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property have a huge potential in the practical utilization for gas separation.
33205576	0	62	theme	Containing	59:68	arg1	Cation					82:87	a New Cellulose Ester Containing Imidazolium Cation	37:87	a New Cellulose Ester Containing Imidazolium Cation for High-Performance CO2 Separation Membranes	37:133	Immobilization of Ionic Liquids with a New Cellulose Ester Containing Imidazolium Cation for High-Performance CO2 Separation Membranes.
33205576	0	63	theme	High-Performance	93:108	arg1	Membranes					125:133	High-Performance CO2 Separation Membranes	93:133	High-Performance CO2 Separation Membranes	93:133	Immobilization of Ionic Liquids with a New Cellulose Ester Containing Imidazolium Cation for High-Performance CO2 Separation Membranes.
33205576	8	64	theme	Such	1299:1302	arg1	membranes					1336:1344	Such high-performance CO2 separation membranes	1299:1344	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property	1299:1420	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property have a huge potential in the practical utilization for gas separation.
33205576	8	65	with	membranes	1336:1344	arg1	property					1413:1420	good mechanical property	1397:1420	good mechanical property	1397:1420	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property have a huge potential in the practical utilization for gas separation.
33205576	8	65	with	membranes	1336:1344	arg1	transparency					1379:1390	high transparency	1374:1390	high transparency	1374:1390	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property have a huge potential in the practical utilization for gas separation.
33205576	8	65	with	membranes	1336:1344	arg1	permeability					1360:1371	high CO2 permeability	1351:1371	high CO2 permeability	1351:1371	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property have a huge potential in the practical utilization for gas separation.
33205576	0	66	theme	Separation	114:123	arg1	Membranes					125:133	High-Performance CO2 Separation Membranes	93:133	High-Performance CO2 Separation Membranes	93:133	Immobilization of Ionic Liquids with a New Cellulose Ester Containing Imidazolium Cation for High-Performance CO2 Separation Membranes.
33205576	5	67	theme	dramatic	963:970	arg1	increase					972:979	the dramatic increase	959:979	the dramatic increase of the CO2 diffusion rate	959:1005	Moreover, the added ILs with a long alkyl chain in the cation and a bis(trifluoromethane sulfonyl)imide anion remarkably improve the CO2 permeability of the cellulose ester/ILs membranes, because of the dramatic increase of the CO2 diffusion rate.
33205576	6	68	theme	CO2	1068:1070	arg1	permeability					1072:1083	the highest CO2 permeability	1056:1083	the highest CO2 permeability	1056:1083	The CA-BmimTf2 N/C10 mimTf2 N membranes exhibit the highest CO2 permeability, which is 3800% higher than that of CA membrane and 1700% higher than that of CA-BmimTf2 N membrane.
33205576	8	69	theme	CO2	1321:1323	arg1	membranes					1336:1344	Such high-performance CO2 separation membranes	1299:1344	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property	1299:1420	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property have a huge potential in the practical utilization for gas separation.
33205576	2	70	theme	1-butyl-3-methylimidazolium	390:416	arg1	imide					448:452	CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide	387:452	CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N)	387:467	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	2	70	theme	1-butyl-3-methylimidazolium	390:416	arg1	N					466:466	CA-BmimTf2 N	455:466	CA-BmimTf2 N	455:466	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	2	70	theme	1-butyl-3-methylimidazolium	390:416	arg1	kinds					262:266	two kinds	258:266	two kinds of new cellulose esters containing imidazolium cation	258:320	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	5	71	theme	ester/ILs	927:935	arg1	membranes					937:945	the cellulose ester/ILs membranes	913:945	the cellulose ester/ILs membranes	913:945	Moreover, the added ILs with a long alkyl chain in the cation and a bis(trifluoromethane sulfonyl)imide anion remarkably improve the CO2 permeability of the cellulose ester/ILs membranes, because of the dramatic increase of the CO2 diffusion rate.
33205576	2	72	theme	CA-BmimTf2	455:464	arg1	imide					448:452	CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide	387:452	CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N)	387:467	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	2	72	theme	CA-BmimTf2	455:464	arg1	N					466:466	CA-BmimTf2 N	455:466	CA-BmimTf2 N	455:466	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	6	73	dep	exhibit	1048:1054	arg1	higher					1101:1106	higher	1101:1106	higher	1101:1106	The CA-BmimTf2 N/C10 mimTf2 N membranes exhibit the highest CO2 permeability, which is 3800% higher than that of CA membrane and 1700% higher than that of CA-BmimTf2 N membrane.
33205576	6	73	dep	exhibit	1048:1054	arg1	higher					1143:1148	higher	1143:1148	higher	1143:1148	The CA-BmimTf2 N/C10 mimTf2 N membranes exhibit the highest CO2 permeability, which is 3800% higher than that of CA membrane and 1700% higher than that of CA-BmimTf2 N membrane.
33205576	0	74	theme	Liquids	24:30	arg1	Immobilization					0:13	Immobilization	0:13	Immobilization of Ionic Liquids with a New Cellulose Ester Containing Imidazolium Cation for High-Performance CO2 Separation Membranes.	0:134	Immobilization of Ionic Liquids with a New Cellulose Ester Containing Imidazolium Cation for High-Performance CO2 Separation Membranes.
33205576	7	75	theme	mechanical	1254:1263	arg1	properties					1265:1274	good mechanical properties	1249:1274	good mechanical properties	1249:1274	More importantly, the CA-BmimTf2 N/C10 mimTf2 N membranes have good mechanical properties and thermal stability.
33205576	8	76	theme	high	1351:1354	arg1	permeability					1360:1371	high CO2 permeability	1351:1371	high CO2 permeability	1351:1371	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property have a huge potential in the practical utilization for gas separation.
33205576	2	77	theme	sulfonyl	439:446	arg1	imide					448:452	CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide	387:452	CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N)	387:467	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	2	77	theme	sulfonyl	439:446	arg1	N					466:466	CA-BmimTf2 N	455:466	CA-BmimTf2 N	455:466	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	2	77	theme	sulfonyl	439:446	arg1	kinds					262:266	two kinds	258:266	two kinds of new cellulose esters containing imidazolium cation	258:320	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	5	78	theme	rate	1002:1005	arg1	increase					972:979	the dramatic increase	959:979	the dramatic increase of the CO2 diffusion rate	959:1005	Moreover, the added ILs with a long alkyl chain in the cation and a bis(trifluoromethane sulfonyl)imide anion remarkably improve the CO2 permeability of the cellulose ester/ILs membranes, because of the dramatic increase of the CO2 diffusion rate.
33205576	6	79	theme	mimTf2	1029:1034	arg1	membranes					1038:1046	The CA-BmimTf2 N/C10 mimTf2 N membranes	1008:1046	The CA-BmimTf2 N/C10 mimTf2 N membranes	1008:1046	The CA-BmimTf2 N/C10 mimTf2 N membranes exhibit the highest CO2 permeability, which is 3800% higher than that of CA membrane and 1700% higher than that of CA-BmimTf2 N membrane.
33205576	5	80	theme	alkyl	796:800	arg1	chain					802:806	a long alkyl chain	789:806	a long alkyl chain	789:806	Moreover, the added ILs with a long alkyl chain in the cation and a bis(trifluoromethane sulfonyl)imide anion remarkably improve the CO2 permeability of the cellulose ester/ILs membranes, because of the dramatic increase of the CO2 diffusion rate.
33205576	2	81	contain	containing	292:301	arg2	cation					315:320	imidazolium cation	303:320	imidazolium cation	303:320	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	2	81	contain	containing	292:301	arg1	esters					285:290	new cellulose esters	271:290	new cellulose esters containing imidazolium cation	271:320	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	7	82	theme	N	1232:1232	arg1	membranes					1234:1242	the CA-BmimTf2 N/C10 mimTf2 N membranes	1204:1242	the CA-BmimTf2 N/C10 mimTf2 N membranes	1204:1242	More importantly, the CA-BmimTf2 N/C10 mimTf2 N membranes have good mechanical properties and thermal stability.
33205576	8	83	theme	high	1374:1377	arg1	transparency					1379:1390	high transparency	1374:1390	high transparency	1374:1390	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property have a huge potential in the practical utilization for gas separation.
33205576	2	84	theme	acetate	333:339	arg1	kinds					262:266	two kinds	258:266	two kinds of new cellulose esters containing imidazolium cation	258:320	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	2	84	theme	acetate	333:339	arg1	chloride					374:381	cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride	323:381	cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride	323:381	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	7	85	theme	N/C10	1219:1223	arg1	membranes					1234:1242	the CA-BmimTf2 N/C10 mimTf2 N membranes	1204:1242	the CA-BmimTf2 N/C10 mimTf2 N membranes	1204:1242	More importantly, the CA-BmimTf2 N/C10 mimTf2 N membranes have good mechanical properties and thermal stability.
33205576	5	86	theme	CO2	988:990	arg1	rate					1002:1005	the CO2 diffusion rate	984:1005	the CO2 diffusion rate	984:1005	Moreover, the added ILs with a long alkyl chain in the cation and a bis(trifluoromethane sulfonyl)imide anion remarkably improve the CO2 permeability of the cellulose ester/ILs membranes, because of the dramatic increase of the CO2 diffusion rate.
33205576	4	87	theme	strong	630:635	arg1	interactions					648:659	the strong attraction interactions	626:659	the strong attraction interactions	626:659	Due to the strong attraction interactions, the obtained cellulose ester/ILs composite membranes are uniform, smooth, and highly transparent.
33205576	8	88	theme	good	1397:1400	arg1	property					1413:1420	good mechanical property	1397:1420	good mechanical property	1397:1420	Such high-performance CO2 separation membranes with high CO2 permeability, high transparency, and good mechanical property have a huge potential in the practical utilization for gas separation.
33205576	2	89	theme	1-butyl-3-methylimidazolium	346:372	arg1	kinds					262:266	two kinds	258:266	two kinds of new cellulose esters containing imidazolium cation	258:320	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33205576	2	89	theme	1-butyl-3-methylimidazolium	346:372	arg1	chloride					374:381	cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride	323:381	cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride	323:381	In this work, two kinds of new cellulose esters containing imidazolium cation, cellulose acetate (CA) 1-butyl-3-methylimidazolium chloride and CA 1-butyl-3-methylimidazolium bis(trifluoromethane sulfonyl)imide (CA-BmimTf2 N), are designed and synthesized.
33774071	0	0	theme	SCFAs	93:97	arg1	metabolism					99:108	SCFAs metabolism	93:108	SCFAs metabolism	93:108	Crataegus pinnatifida polysaccharide alleviates colitis via modulation of gut microbiota and SCFAs metabolism.
33774071	4	1	theme	dextran	483:489	arg1	DSS					508:510	DSS	508:510	DSS	508:510	Here, we established a direct link between colitis induced by dextran sulphate sodium (DSS) in mice and polysaccharide HAW1-2 isolated from hawthorn.
33774071	4	1	theme	dextran	483:489	arg1	sodium					500:505	dextran sulphate sodium	483:505	dextran sulphate sodium (DSS) in mice and polysaccharide HAW1-2 isolated from hawthorn	483:568	Here, we established a direct link between colitis induced by dextran sulphate sodium (DSS) in mice and polysaccharide HAW1-2 isolated from hawthorn.
33774071	5	2	theme	cytokines	685:693	arg1	expression					658:667	the expression	654:667	the expression of inflammatory cytokines including IL-1β, IL-6 and TNF-α	654:725	Our results showed HAW1-2 restored the pathological lesions in colon and inhibited the expression of inflammatory cytokines including IL-1β, IL-6 and TNF-α.
33774071	7	3	theme	colon	888:892	arg1	inflammation					872:883	the inflammation	868:883	the inflammation of colon	868:892	These findings suggested HAW1-2 could alleviate the inflammation of colon.
33774071	10	4	theme	anti-inflammation	1240:1256	arg1	activity					1258:1265	the anti-inflammation activity	1236:1265	the anti-inflammation activity of HAW1-2	1236:1275	Finally, we demonstrated the anti-inflammation activity of HAW1-2 might be induced by acetic acid.
33774071	9	5	theme	inflammatory	1187:1198	arg1	cytokines					1200:1208	inflammatory cytokines	1187:1208	inflammatory cytokines	1187:1208	Besides, we showed Alistipes and Odoribacter were positively co-related with acetic acid and propionic acid while were negatively co-related with inflammatory cytokines.
33774071	4	6	from	sodium	500:505	arg1	mice					516:519	mice	516:519	mice	516:519	Here, we established a direct link between colitis induced by dextran sulphate sodium (DSS) in mice and polysaccharide HAW1-2 isolated from hawthorn.
33774071	4	6	from	sodium	500:505	arg1	HAW1-2					540:545	polysaccharide HAW1-2	525:545	polysaccharide HAW1-2	525:545	Here, we established a direct link between colitis induced by dextran sulphate sodium (DSS) in mice and polysaccharide HAW1-2 isolated from hawthorn.
33774071	12	7	theme	microbiota-directed	1478:1496	arg1	intervention					1498:1509	microbiota-directed intervention	1478:1509	microbiota-directed intervention in IBD patients	1478:1525	It also implies microbiota-directed intervention in IBD patients should be particularly given more attention.
33774071	1	8	dep	individual	154:163	arg1	medications					174:184	medications	174:184	medications	174:184	Inflammatory bowel disease (IBD) afflicted individual and most medications have side-effects.
33774071	1	8	dep	individual	154:163	arg1	have					186:189	have	186:189	have side-effects	186:202	Inflammatory bowel disease (IBD) afflicted individual and most medications have side-effects.
33774071	0	9	theme	metabolism	99:108	arg1	modulation					60:69	modulation	60:69	modulation of gut microbiota and SCFAs metabolism	60:108	Crataegus pinnatifida polysaccharide alleviates colitis via modulation of gut microbiota and SCFAs metabolism.
33774071	0	10	theme	Crataegus	0:8	arg1	polysaccharide					22:35	Crataegus pinnatifida polysaccharide	0:35	Crataegus pinnatifida polysaccharide	0:35	Crataegus pinnatifida polysaccharide alleviates colitis via modulation of gut microbiota and SCFAs metabolism.
33774071	6	11	theme	phosphorylation	767:781	arg1	levels					783:788	the phosphorylation levels	763:788	the phosphorylation levels	763:788	Meanwhile, IKKα/β, IκBα, NF-κB and the phosphorylation levels were inhibited significantly.
33774071	2	12	theme	homolog	273:279	arg1	plant					281:285	a safe medicine and food homolog plant	248:285	plant	281:285	Crataegus pinnatifida (Hawthorn), which is a safe medicine and food homolog plant, has been reported to prevent colitis in murine.
33774071	5	13	theme	pathological	610:621	arg1	lesions					623:629	the pathological lesions	606:629	the pathological lesions in colon	606:638	Our results showed HAW1-2 restored the pathological lesions in colon and inhibited the expression of inflammatory cytokines including IL-1β, IL-6 and TNF-α.
33774071	2	14	theme	food	268:271	arg1	plant					281:285	a safe medicine and food homolog plant	248:285	plant	281:285	Crataegus pinnatifida (Hawthorn), which is a safe medicine and food homolog plant, has been reported to prevent colitis in murine.
33774071	0	15	theme	pinnatifida	10:20	arg1	polysaccharide					22:35	Crataegus pinnatifida polysaccharide	0:35	Crataegus pinnatifida polysaccharide	0:35	Crataegus pinnatifida polysaccharide alleviates colitis via modulation of gut microbiota and SCFAs metabolism.
33774071	5	16	from	lesions	623:629	arg1	colon					634:638	colon	634:638	colon	634:638	Our results showed HAW1-2 restored the pathological lesions in colon and inhibited the expression of inflammatory cytokines including IL-1β, IL-6 and TNF-α.
33774071	3	17	theme	underlying	374:383	arg1	mechanism					395:403	the underlying molecular mechanism	370:403	the underlying molecular mechanism	370:403	Yet the bioactivity component and the underlying molecular mechanism remain unclear.
33774071	4	18	theme	polysaccharide	525:538	arg1	HAW1-2					540:545	polysaccharide HAW1-2	525:545	polysaccharide HAW1-2	525:545	Here, we established a direct link between colitis induced by dextran sulphate sodium (DSS) in mice and polysaccharide HAW1-2 isolated from hawthorn.
33774071	11	19	theme	present	1324:1330	arg1	data					1332:1335	the present data	1320:1335	the present data	1320:1335	Together, the present data revealed HAW1-2 could directly modify the gut microbiota, especially for Bacteroides, and generate SCFAs to inhibit colitis.
33774071	12	20	from	intervention	1498:1509	arg1	patients					1518:1525	IBD patients	1514:1525	IBD patients	1514:1525	It also implies microbiota-directed intervention in IBD patients should be particularly given more attention.
33774071	9	21	dep	showed	1053:1058	arg1	co-related					1102:1111	co-related	1102:1111	co-related	1102:1111	Besides, we showed Alistipes and Odoribacter were positively co-related with acetic acid and propionic acid while were negatively co-related with inflammatory cytokines.
33774071	1	22	theme	Inflammatory	111:122	arg1	IBD					139:141	IBD	139:141	IBD	139:141	Inflammatory bowel disease (IBD) afflicted individual and most medications have side-effects.
33774071	1	22	theme	Inflammatory	111:122	arg1	disease					130:136	Inflammatory bowel disease	111:136	Inflammatory bowel disease (IBD)	111:142	Inflammatory bowel disease (IBD) afflicted individual and most medications have side-effects.
33774071	9	23	theme	acetic	1118:1123	arg1	acid					1125:1128	acetic acid	1118:1128	acetic acid	1118:1128	Besides, we showed Alistipes and Odoribacter were positively co-related with acetic acid and propionic acid while were negatively co-related with inflammatory cytokines.
33774071	1	24	theme	bowel	124:128	arg1	IBD					139:141	IBD	139:141	IBD	139:141	Inflammatory bowel disease (IBD) afflicted individual and most medications have side-effects.
33774071	1	24	theme	bowel	124:128	arg1	disease					130:136	Inflammatory bowel disease	111:136	Inflammatory bowel disease (IBD)	111:142	Inflammatory bowel disease (IBD) afflicted individual and most medications have side-effects.
33774071	12	25	theme	more	1556:1559	arg1	attention					1561:1569	more attention	1556:1569	more attention	1556:1569	It also implies microbiota-directed intervention in IBD patients should be particularly given more attention.
33774071	7	26	dep	suggested	835:843	arg1	alleviate					858:866	alleviate	858:866	suggested HAW1-2 could alleviate the inflammation of colon	835:892	These findings suggested HAW1-2 could alleviate the inflammation of colon.
33774071	3	27	theme	molecular	385:393	arg1	mechanism					395:403	the underlying molecular mechanism	370:403	the underlying molecular mechanism	370:403	Yet the bioactivity component and the underlying molecular mechanism remain unclear.
33774071	4	28	theme	direct	444:449	arg1	link					451:454	a direct link	442:454	a direct link between colitis induced by dextran sulphate sodium (DSS) in mice and polysaccharide HAW1-2 isolated from hawthorn	442:568	Here, we established a direct link between colitis induced by dextran sulphate sodium (DSS) in mice and polysaccharide HAW1-2 isolated from hawthorn.
33774071	11	29	theme	gut	1379:1381	arg1	microbiota					1383:1392	the gut microbiota	1375:1392	the gut microbiota	1375:1392	Together, the present data revealed HAW1-2 could directly modify the gut microbiota, especially for Bacteroides, and generate SCFAs to inhibit colitis.
33774071	12	30	theme	IBD	1514:1516	arg1	patients					1518:1525	IBD patients	1514:1525	IBD patients	1514:1525	It also implies microbiota-directed intervention in IBD patients should be particularly given more attention.
33774071	5	31	theme	inflammatory	672:683	arg1	IL-1β					705:709	IL-1β	705:709	IL-1β	705:709	Our results showed HAW1-2 restored the pathological lesions in colon and inhibited the expression of inflammatory cytokines including IL-1β, IL-6 and TNF-α.
33774071	5	31	theme	inflammatory	672:683	arg1	cytokines					685:693	inflammatory cytokines	672:693	inflammatory cytokines including IL-1β, IL-6 and TNF-α	672:725	Our results showed HAW1-2 restored the pathological lesions in colon and inhibited the expression of inflammatory cytokines including IL-1β, IL-6 and TNF-α.
33774071	5	31	theme	inflammatory	672:683	arg1	TNF-α					721:725	TNF-α	721:725	TNF-α	721:725	Our results showed HAW1-2 restored the pathological lesions in colon and inhibited the expression of inflammatory cytokines including IL-1β, IL-6 and TNF-α.
33774071	5	31	theme	inflammatory	672:683	arg1	IL-6					712:715	IL-6	712:715	IL-6	712:715	Our results showed HAW1-2 restored the pathological lesions in colon and inhibited the expression of inflammatory cytokines including IL-1β, IL-6 and TNF-α.
33774071	9	32	with	co-related	1102:1111	arg1	acid					1125:1128	acetic acid	1118:1128	acetic acid	1118:1128	Besides, we showed Alistipes and Odoribacter were positively co-related with acetic acid and propionic acid while were negatively co-related with inflammatory cytokines.
33774071	9	32	with	co-related	1102:1111	arg1	acid					1144:1147	propionic acid	1134:1147	propionic acid	1134:1147	Besides, we showed Alistipes and Odoribacter were positively co-related with acetic acid and propionic acid while were negatively co-related with inflammatory cytokines.
33774071	10	33	theme	HAW1-2	1270:1275	arg1	activity					1258:1265	the anti-inflammation activity	1236:1265	the anti-inflammation activity of HAW1-2	1236:1275	Finally, we demonstrated the anti-inflammation activity of HAW1-2 might be induced by acetic acid.
33774071	4	34	attach	isolated	547:554	arg2	mice					516:519	mice	516:519	mice	516:519	Here, we established a direct link between colitis induced by dextran sulphate sodium (DSS) in mice and polysaccharide HAW1-2 isolated from hawthorn.
33774071	4	34	attach	isolated	547:554	arg1	hawthorn					561:568	hawthorn	561:568	hawthorn	561:568	Here, we established a direct link between colitis induced by dextran sulphate sodium (DSS) in mice and polysaccharide HAW1-2 isolated from hawthorn.
33774071	9	35	theme	propionic	1134:1142	arg1	acid					1144:1147	propionic acid	1134:1147	propionic acid	1134:1147	Besides, we showed Alistipes and Odoribacter were positively co-related with acetic acid and propionic acid while were negatively co-related with inflammatory cytokines.
33774071	8	36	theme	microbiota	936:945	arg1	composition					917:927	the composition	913:927	the composition of gut microbiota	913:945	Further, we found the composition of gut microbiota was modified and Bacteroides including Alistipes and Odoribacter were significantly enriched.
33774071	0	37	theme	microbiota	78:87	arg1	modulation					60:69	modulation	60:69	modulation of gut microbiota and SCFAs metabolism	60:108	Crataegus pinnatifida polysaccharide alleviates colitis via modulation of gut microbiota and SCFAs metabolism.
33774071	2	38	theme	safe	250:253	arg1	pinnatifida					215:225	pinnatifida	215:225	pinnatifida	215:225	Crataegus pinnatifida (Hawthorn), which is a safe medicine and food homolog plant, has been reported to prevent colitis in murine.
33774071	2	38	theme	safe	250:253	arg1	medicine					255:262	a safe medicine and food homolog plant	248:285	medicine	255:262	Crataegus pinnatifida (Hawthorn), which is a safe medicine and food homolog plant, has been reported to prevent colitis in murine.
33774071	8	39	theme	gut	932:934	arg1	microbiota					936:945	gut microbiota	932:945	gut microbiota	932:945	Further, we found the composition of gut microbiota was modified and Bacteroides including Alistipes and Odoribacter were significantly enriched.
33774071	5	40	dep	showed	583:588	arg1	inhibited					644:652	inhibited	644:652	inhibited the expression of inflammatory cytokines including IL-1β, IL-6 and TNF-α	644:725	Our results showed HAW1-2 restored the pathological lesions in colon and inhibited the expression of inflammatory cytokines including IL-1β, IL-6 and TNF-α.
33774071	5	40	dep	showed	583:588	arg1	restored					597:604	restored	597:604	restored the pathological lesions in colon	597:638	Our results showed HAW1-2 restored the pathological lesions in colon and inhibited the expression of inflammatory cytokines including IL-1β, IL-6 and TNF-α.
33774071	0	41	theme	gut	74:76	arg1	microbiota					78:87	gut microbiota	74:87	gut microbiota	74:87	Crataegus pinnatifida polysaccharide alleviates colitis via modulation of gut microbiota and SCFAs metabolism.
33774071	4	42	theme	sulphate	491:498	arg1	DSS					508:510	DSS	508:510	DSS	508:510	Here, we established a direct link between colitis induced by dextran sulphate sodium (DSS) in mice and polysaccharide HAW1-2 isolated from hawthorn.
33774071	4	42	theme	sulphate	491:498	arg1	sodium					500:505	dextran sulphate sodium	483:505	dextran sulphate sodium (DSS) in mice and polysaccharide HAW1-2 isolated from hawthorn	483:568	Here, we established a direct link between colitis induced by dextran sulphate sodium (DSS) in mice and polysaccharide HAW1-2 isolated from hawthorn.
33774071	10	43	theme	acetic	1297:1302	arg1	acid					1304:1307	acetic acid	1297:1307	acetic acid	1297:1307	Finally, we demonstrated the anti-inflammation activity of HAW1-2 might be induced by acetic acid.
33774071	3	44	theme	bioactivity	344:354	arg1	component					356:364	the bioactivity component	340:364	the bioactivity component	340:364	Yet the bioactivity component and the underlying molecular mechanism remain unclear.
34730621	10	0	theme	first	1391:1395	arg1	this					1379:1382	this	1379:1382	this	1379:1382	To our knowledge, this is the first time that mannogalactans are isolated from S. commune liquid culture.
34730621	10	0	theme	first	1391:1395	arg1	time					1397:1400	the first time	1387:1400	the first time that mannogalactans are isolated from S. commune liquid culture	1387:1464	To our knowledge, this is the first time that mannogalactans are isolated from S. commune liquid culture.
34730621	5	1	theme	cell	720:723	arg1	wall					725:728	cell wall	720:728	cell wall of wild (ScW) and mutants	720:754	Polysaccharides from cell wall of wild (ScW) and mutants were compared in this study.
34730621	10	2	attach	isolated	1426:1433	arg1	culture					1458:1464	S. commune liquid culture	1440:1464	S. commune liquid culture	1440:1464	To our knowledge, this is the first time that mannogalactans are isolated from S. commune liquid culture.
34730621	10	2	attach	isolated	1426:1433	arg2	mannogalactans					1407:1420	mannogalactans	1407:1420	mannogalactans	1407:1420	To our knowledge, this is the first time that mannogalactans are isolated from S. commune liquid culture.
34730621	7	3	theme	wild	1101:1104	arg1	type					1106:1109	the wild type	1097:1109	the wild type	1097:1109	One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type.
34730621	6	4	theme	culture	822:828	arg1	broth					830:834	the biomass and culture broth	806:834	broth	830:834	Polysaccharides from the biomass and culture broth were extracted with hot water.
34730621	1	5	theme	medicinal	175:183	arg1	properties					231:240	pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties	159:240	pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties	159:240	The Agaricomycetes fungi produce various compounds with pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties.
34730621	10	6	theme	liquid	1451:1456	arg1	culture					1458:1464	S. commune liquid culture	1440:1464	S. commune liquid culture	1440:1464	To our knowledge, this is the first time that mannogalactans are isolated from S. commune liquid culture.
34730621	3	7	theme	wall	549:552	arg1	polysaccharides					554:568	the cell wall polysaccharides	540:568	the cell wall polysaccharides	540:568	The aim of this study was to use genetic modification to transform Schizophyllum commune and identify if the phenotype observed (different from the wild type) resulted in changes of the cell wall polysaccharides.
34730621	2	8	theme	antitumor	318:326	arg1	actions					349:355	antitumor and immunomodulatory actions	318:355	antitumor and immunomodulatory actions	318:355	In addition, some polysaccharides extracted from the fungal cell wall have antitumor and immunomodulatory actions.
34730621	5	9	from	wall	725:728	arg1	Polysaccharides					699:713	Polysaccharides	699:713	Polysaccharides from cell wall of wild (ScW) and mutants	699:754	Polysaccharides from cell wall of wild (ScW) and mutants were compared in this study.
34730621	9	10	theme	S.	1283:1284	arg1	wall					1299:1302	S. commune cell wall	1283:1302	S. commune cell wall	1283:1302	From the monosaccharide composition it was proposed that mannogalactans are components of S. commune cell wall for both, wild and mutant, but in different proportions.
34730621	6	11	theme	biomass	810:816	arg1	broth					830:834	the biomass and culture broth	806:834	broth	830:834	Polysaccharides from the biomass and culture broth were extracted with hot water.
34730621	3	12	theme	polysaccharides	554:568	arg1	changes					529:535	changes	529:535	changes of the cell wall polysaccharides	529:568	The aim of this study was to use genetic modification to transform Schizophyllum commune and identify if the phenotype observed (different from the wild type) resulted in changes of the cell wall polysaccharides.
34730621	9	13	from	components	1269:1278	arg1	proportions					1348:1358	different proportions	1338:1358	different proportions	1338:1358	From the monosaccharide composition it was proposed that mannogalactans are components of S. commune cell wall for both, wild and mutant, but in different proportions.
34730621	8	14	from	analyses	1183:1190	arg1	profiles					1155:1162	the elution profiles	1143:1162	the elution profiles from HPSEC and NMR analyses	1143:1190	Differences were also found in the elution profiles from HPSEC and NMR analyses.
34730621	2	15	theme	cell	303:306	arg1	wall					308:311	the fungal cell wall	292:311	the fungal cell wall	292:311	In addition, some polysaccharides extracted from the fungal cell wall have antitumor and immunomodulatory actions.
34730621	4	16	theme	S.	671:672	arg1	transformations					682:696	S. commune transformations	671:696	S. commune transformations	671:696	The plasmid pUCHYG-GPDGLS, which contains the Pleurotus ostreatus glucan synthase gene, was used in S. commune transformations.
34730621	3	17	theme	study	374:378	arg1	aim					362:364	The aim	358:364	The aim of this study	358:378	The aim of this study was to use genetic modification to transform Schizophyllum commune and identify if the phenotype observed (different from the wild type) resulted in changes of the cell wall polysaccharides.
34730621	9	18	theme	cell	1294:1297	arg1	wall					1299:1302	S. commune cell wall	1283:1302	S. commune cell wall	1283:1302	From the monosaccharide composition it was proposed that mannogalactans are components of S. commune cell wall for both, wild and mutant, but in different proportions.
34730621	1	19	with	compounds	144:152	arg1	properties					231:240	pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties	159:240	pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties	159:240	The Agaricomycetes fungi produce various compounds with pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties.
34730621	2	20	theme	fungal	296:301	arg1	wall					308:311	the fungal cell wall	292:311	the fungal cell wall	292:311	In addition, some polysaccharides extracted from the fungal cell wall have antitumor and immunomodulatory actions.
34730621	10	21	dep	time	1397:1400	arg1	isolated					1426:1433	isolated	1426:1433	are isolated from S. commune liquid culture	1422:1464	To our knowledge, this is the first time that mannogalactans are isolated from S. commune liquid culture.
34730621	1	22	theme	cosmetic	186:193	arg1	properties					231:240	pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties	159:240	pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties	159:240	The Agaricomycetes fungi produce various compounds with pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties.
34730621	4	23	theme	plasmid	575:581	arg1	pUCHYG-GPDGLS					583:595	The plasmid pUCHYG-GPDGLS	571:595	The plasmid pUCHYG-GPDGLS	571:595	The plasmid pUCHYG-GPDGLS, which contains the Pleurotus ostreatus glucan synthase gene, was used in S. commune transformations.
34730621	9	24	theme	commune	1286:1292	arg1	wall					1299:1302	S. commune cell wall	1283:1302	S. commune cell wall	1283:1302	From the monosaccharide composition it was proposed that mannogalactans are components of S. commune cell wall for both, wild and mutant, but in different proportions.
34730621	8	25	theme	elution	1147:1153	arg1	profiles					1155:1162	the elution profiles	1143:1162	the elution profiles from HPSEC and NMR analyses	1143:1190	Differences were also found in the elution profiles from HPSEC and NMR analyses.
34730621	7	26	from	glucose	1065:1071	arg1	fraction					1036:1043	polysaccharide fraction	1021:1043	polysaccharide fraction from the mutant	1021:1059	One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type.
34730621	7	26	from	glucose	1065:1071	arg1	mutant					1054:1059	the mutant	1050:1059	the mutant	1050:1059	One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type.
34730621	9	27	theme	wall	1299:1302	arg1	components					1269:1278	components	1269:1278	components of S. commune cell wall	1269:1302	From the monosaccharide composition it was proposed that mannogalactans are components of S. commune cell wall for both, wild and mutant, but in different proportions.
34730621	9	27	theme	wall	1299:1302	arg1	mannogalactans					1250:1263	mannogalactans	1250:1263	mannogalactans	1250:1263	From the monosaccharide composition it was proposed that mannogalactans are components of S. commune cell wall for both, wild and mutant, but in different proportions.
34730621	3	28	from	type	511:514	arg1	different					487:495	different	487:495	different	487:495	The aim of this study was to use genetic modification to transform Schizophyllum commune and identify if the phenotype observed (different from the wild type) resulted in changes of the cell wall polysaccharides.
34730621	2	29	theme	immunomodulatory	332:347	arg1	actions					349:355	antitumor and immunomodulatory actions	318:355	antitumor and immunomodulatory actions	318:355	In addition, some polysaccharides extracted from the fungal cell wall have antitumor and immunomodulatory actions.
34730621	1	30	theme	environmental	196:208	arg1	properties					231:240	pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties	159:240	pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties	159:240	The Agaricomycetes fungi produce various compounds with pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties.
34730621	0	31	theme	wall	19:22	arg1	polysaccharides					24:38	cell wall polysaccharides	14:38	cell wall polysaccharides	14:38	Comparison of cell wall polysaccharides in Schizophyllum commune after changing phenotype by mutation.
34730621	10	32	theme	commune	1443:1449	arg1	culture					1458:1464	S. commune liquid culture	1440:1464	S. commune liquid culture	1440:1464	To our knowledge, this is the first time that mannogalactans are isolated from S. commune liquid culture.
34730621	7	33	theme	further	910:916	arg1	studies					918:924	further studies	910:924	further studies	910:924	One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type.
34730621	3	34	theme	different	487:495	arg1	phenotype					467:475	the phenotype	463:475	the phenotype observed (different from the wild type)	463:515	The aim of this study was to use genetic modification to transform Schizophyllum commune and identify if the phenotype observed (different from the wild type) resulted in changes of the cell wall polysaccharides.
34730621	3	35	theme	genetic	391:397	arg1	modification					399:410	genetic modification	391:410	genetic modification	391:410	The aim of this study was to use genetic modification to transform Schizophyllum commune and identify if the phenotype observed (different from the wild type) resulted in changes of the cell wall polysaccharides.
34730621	10	36	theme	S.	1440:1441	arg1	commune					1443:1449	S. commune	1440:1449	S. commune liquid culture	1440:1464	To our knowledge, this is the first time that mannogalactans are isolated from S. commune liquid culture.
34730621	1	37	theme	Agaricomycetes	107:120	arg1	fungi					122:126	The Agaricomycetes fungi	103:126	The Agaricomycetes fungi	103:126	The Agaricomycetes fungi produce various compounds with pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties.
34730621	1	38	theme	biotechnological	214:229	arg1	properties					231:240	pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties	159:240	pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties	159:240	The Agaricomycetes fungi produce various compounds with pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties.
34730621	8	39	theme	NMR	1179:1181	arg1	analyses					1183:1190	NMR analyses	1179:1190	NMR analyses	1179:1190	Differences were also found in the elution profiles from HPSEC and NMR analyses.
34730621	2	40	contain	have	313:316	arg2	actions					349:355	antitumor and immunomodulatory actions	318:355	antitumor and immunomodulatory actions	318:355	In addition, some polysaccharides extracted from the fungal cell wall have antitumor and immunomodulatory actions.
34730621	2	40	contain	have	313:316	arg1	polysaccharides					261:275	some polysaccharides	256:275	some polysaccharides extracted from the fungal cell wall	256:311	In addition, some polysaccharides extracted from the fungal cell wall have antitumor and immunomodulatory actions.
34730621	5	41	theme	wild	733:736	arg1	wall					725:728	cell wall	720:728	cell wall of wild (ScW) and mutants	720:754	Polysaccharides from cell wall of wild (ScW) and mutants were compared in this study.
34730621	4	42	contain	contains	604:611	arg2	gene					653:656	the Pleurotus ostreatus glucan synthase gene	613:656	the Pleurotus ostreatus glucan synthase gene	613:656	The plasmid pUCHYG-GPDGLS, which contains the Pleurotus ostreatus glucan synthase gene, was used in S. commune transformations.
34730621	4	42	contain	contains	604:611	arg1	pUCHYG-GPDGLS					583:595	The plasmid pUCHYG-GPDGLS	571:595	The plasmid pUCHYG-GPDGLS	571:595	The plasmid pUCHYG-GPDGLS, which contains the Pleurotus ostreatus glucan synthase gene, was used in S. commune transformations.
34730621	4	43	theme	commune	674:680	arg1	transformations					682:696	S. commune transformations	671:696	S. commune transformations	671:696	The plasmid pUCHYG-GPDGLS, which contains the Pleurotus ostreatus glucan synthase gene, was used in S. commune transformations.
34730621	0	44	theme	polysaccharides	24:38	arg1	Comparison					0:9	Comparison	0:9	Comparison of cell wall polysaccharides in Schizophyllum	0:55	Comparison of cell wall polysaccharides in Schizophyllum commune after changing phenotype by mutation.
34730621	7	45	from	monomer	1086:1092	arg1	type					1106:1109	the wild type	1097:1109	the wild type	1097:1109	One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type.
34730621	6	46	from	broth	830:834	arg1	Polysaccharides					785:799	Polysaccharides	785:799	Polysaccharides from the biomass and culture broth	785:834	Polysaccharides from the biomass and culture broth were extracted with hot water.
34730621	9	47	theme	monosaccharide	1202:1215	arg1	composition					1217:1227	the monosaccharide composition	1198:1227	the monosaccharide composition	1198:1227	From the monosaccharide composition it was proposed that mannogalactans are components of S. commune cell wall for both, wild and mutant, but in different proportions.
34730621	5	48	theme	mutants	748:754	arg1	wall					725:728	cell wall	720:728	cell wall of wild (ScW) and mutants	720:754	Polysaccharides from cell wall of wild (ScW) and mutants were compared in this study.
34730621	7	49	theme	polysaccharide	1021:1034	arg1	fraction					1036:1043	polysaccharide fraction	1021:1043	polysaccharide fraction from the mutant	1021:1059	One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type.
34730621	6	50	theme	hot	856:858	arg1	water					860:864	hot water	856:864	hot water	856:864	Polysaccharides from the biomass and culture broth were extracted with hot water.
34730621	7	51	theme	major	1002:1006	arg1	component					1008:1016	the major component	998:1016	the major component in polysaccharide fraction from the mutant	998:1059	One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type.
34730621	7	51	theme	major	1002:1006	arg1	galactose					985:993	galactose	985:993	galactose	985:993	One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type.
34730621	3	52	theme	Schizophyllum	425:437	arg1	commune					439:445	Schizophyllum commune	425:445	Schizophyllum commune	425:445	The aim of this study was to use genetic modification to transform Schizophyllum commune and identify if the phenotype observed (different from the wild type) resulted in changes of the cell wall polysaccharides.
34730621	7	53	theme	GLC	965:967	arg1	analysis					969:976	the GLC analysis	961:976	the GLC analysis	961:976	One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type.
34730621	9	54	theme	different	1338:1346	arg1	proportions					1348:1358	different proportions	1338:1358	different proportions	1338:1358	From the monosaccharide composition it was proposed that mannogalactans are components of S. commune cell wall for both, wild and mutant, but in different proportions.
34730621	8	55	located	found	1134:1138	arg1	profiles					1155:1162	the elution profiles	1143:1162	the elution profiles from HPSEC and NMR analyses	1143:1190	Differences were also found in the elution profiles from HPSEC and NMR analyses.
34730621	8	55	located	found	1134:1138	arg2	Differences					1112:1122	Differences	1112:1122	Differences	1112:1122	Differences were also found in the elution profiles from HPSEC and NMR analyses.
34730621	7	56	theme	major	1080:1084	arg1	galactose					985:993	galactose	985:993	galactose	985:993	One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type.
34730621	7	56	theme	major	1080:1084	arg1	monomer					1086:1092	the major monomer	1076:1092	the major monomer in the wild type	1076:1109	One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type.
34730621	1	57	theme	various	136:142	arg1	compounds					144:152	various compounds	136:152	various compounds with pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties	136:240	The Agaricomycetes fungi produce various compounds with pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties.
34730621	7	58	from	component	1008:1016	arg1	fraction					1036:1043	polysaccharide fraction	1021:1043	polysaccharide fraction from the mutant	1021:1059	One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type.
34730621	7	58	from	component	1008:1016	arg1	mutant					1054:1059	the mutant	1050:1059	the mutant	1050:1059	One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type.
34730621	3	59	theme	cell	544:547	arg1	polysaccharides					554:568	the cell wall polysaccharides	540:568	the cell wall polysaccharides	540:568	The aim of this study was to use genetic modification to transform Schizophyllum commune and identify if the phenotype observed (different from the wild type) resulted in changes of the cell wall polysaccharides.
34730621	3	60	theme	wild	506:509	arg1	type					511:514	the wild type	502:514	the wild type	502:514	The aim of this study was to use genetic modification to transform Schizophyllum commune and identify if the phenotype observed (different from the wild type) resulted in changes of the cell wall polysaccharides.
34730621	4	61	theme	Pleurotus	617:625	arg1	gene					653:656	the Pleurotus ostreatus glucan synthase gene	613:656	the Pleurotus ostreatus glucan synthase gene	613:656	The plasmid pUCHYG-GPDGLS, which contains the Pleurotus ostreatus glucan synthase gene, was used in S. commune transformations.
34730621	4	62	dep	Pleurotus	617:625	arg1	ostreatus					627:635	ostreatus	627:635	ostreatus	627:635	The plasmid pUCHYG-GPDGLS, which contains the Pleurotus ostreatus glucan synthase gene, was used in S. commune transformations.
34730621	4	63	used	used	663:666	arg2	pUCHYG-GPDGLS					583:595	The plasmid pUCHYG-GPDGLS	571:595	The plasmid pUCHYG-GPDGLS	571:595	The plasmid pUCHYG-GPDGLS, which contains the Pleurotus ostreatus glucan synthase gene, was used in S. commune transformations.
34730621	4	64	theme	synthase	644:651	arg1	gene					653:656	the Pleurotus ostreatus glucan synthase gene	613:656	the Pleurotus ostreatus glucan synthase gene	613:656	The plasmid pUCHYG-GPDGLS, which contains the Pleurotus ostreatus glucan synthase gene, was used in S. commune transformations.
34730621	7	65	from	mutant	1054:1059	arg1	component					1008:1016	the major component	998:1016	the major component in polysaccharide fraction from the mutant	998:1059	One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type.
34730621	7	65	from	mutant	1054:1059	arg1	fraction					1036:1043	polysaccharide fraction	1021:1043	polysaccharide fraction from the mutant	1021:1059	One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type.
34730621	7	65	from	mutant	1054:1059	arg1	glucose					1065:1071	glucose	1065:1071	glucose	1065:1071	One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type.
34730621	7	65	from	mutant	1054:1059	arg1	galactose					985:993	galactose	985:993	galactose	985:993	One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type.
34730621	8	66	from	HPSEC	1169:1173	arg1	profiles					1155:1162	the elution profiles	1143:1162	the elution profiles from HPSEC and NMR analyses	1143:1190	Differences were also found in the elution profiles from HPSEC and NMR analyses.
34730621	0	67	theme	cell	14:17	arg1	polysaccharides					24:38	cell wall polysaccharides	14:38	cell wall polysaccharides	14:38	Comparison of cell wall polysaccharides in Schizophyllum commune after changing phenotype by mutation.
34730621	1	68	theme	pharmaceutical	159:172	arg1	properties					231:240	pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties	159:240	pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties	159:240	The Agaricomycetes fungi produce various compounds with pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties.
34730621	7	69	theme	mutants	878:884	arg1	mutants					878:884	the mutants	874:884	the mutants (ScT4)	874:891	One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type.
34730621	7	69	theme	mutants	878:884	arg1	ScT4					887:890	ScT4	887:890	ScT4	887:890	One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type.
34730621	7	69	theme	mutants	878:884	arg1	One					867:869	One	867:869	One	867:869	One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type.
34730621	4	70	theme	glucan	637:642	arg1	gene					653:656	the Pleurotus ostreatus glucan synthase gene	613:656	the Pleurotus ostreatus glucan synthase gene	613:656	The plasmid pUCHYG-GPDGLS, which contains the Pleurotus ostreatus glucan synthase gene, was used in S. commune transformations.
33255011	8	0	theme	sponge	1071:1076	arg1	biocompatibility					1037:1052	Good biocompatibility	1032:1052	Good biocompatibility of the composite sponge	1032:1076	Good biocompatibility of the composite sponge is proved by the results of hemocompatibility and cytotoxicity, indicating an excellent candidate as a rapid hemostatic material.
33255011	6	1	theme	chitosan/cellulose	801:818	arg1	sponge					830:835	the chitosan/cellulose composite sponge	797:835	the chitosan/cellulose composite sponge	797:835	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
33255011	6	2	theme	dynamic	748:754	arg1	time					777:780	the dynamic whole blood clotting time	744:780	the dynamic whole blood clotting time	744:780	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
33255011	4	3	theme	mechanical	583:592	arg1	properties					594:603	good mechanical properties	578:603	good mechanical properties	578:603	The introduction of chitosan endows the chitosan/cellulose composite sponge with good mechanical properties.
33255011	4	4	theme	good	578:581	arg1	properties					594:603	good mechanical properties	578:603	good mechanical properties	578:603	The introduction of chitosan endows the chitosan/cellulose composite sponge with good mechanical properties.
33255011	8	5	theme	excellent	1156:1164	arg1	candidate					1166:1174	an excellent candidate	1153:1174	an excellent candidate as a rapid hemostatic material	1153:1205	Good biocompatibility of the composite sponge is proved by the results of hemocompatibility and cytotoxicity, indicating an excellent candidate as a rapid hemostatic material.
33255011	2	6	theme	hydrophilic	308:318	arg1	sponges					330:336	porous and hydrophilic cellulose sponges	297:336	porous and hydrophilic cellulose sponges using surfactant and pore-foaming agent	297:376	Here, a simple and efficient strategy to produce porous and hydrophilic cellulose sponges using surfactant and pore-foaming agent is demonstrated.
33255011	0	7	dep	surfactant	81:90	arg1	agent					109:113	agent	109:113	agent	109:113	Morphology-controllable cellulose/chitosan sponge for deep wound hemostasis with surfactant and pore-foaming agent.
33255011	3	8	theme	obtained	399:406	arg1	sponges					418:424	The obtained cellulose sponges	395:424	The obtained cellulose sponges	395:424	The obtained cellulose sponges exhibit high water absorption capacity and rapid shape recoverability.
33255011	6	9	theme	gelatin	904:910	arg1	sponge					912:917	gelatin sponge	904:917	gelatin sponge	904:917	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
33255011	7	10	theme	Animal	920:925	arg1	experiment					927:936	Animal experiment	920:936	Animal experiment	920:936	Animal experiment further showed that rapid hemostasis within 105 s could be reached with the composite sponge.
33255011	7	11	theme	rapid	958:962	arg1	hemostasis					964:973	rapid hemostasis	958:973	rapid hemostasis within 105 s	958:986	Animal experiment further showed that rapid hemostasis within 105 s could be reached with the composite sponge.
33255011	2	12	theme	porous	297:302	arg1	sponges					330:336	porous and hydrophilic cellulose sponges	297:336	porous and hydrophilic cellulose sponges using surfactant and pore-foaming agent	297:376	Here, a simple and efficient strategy to produce porous and hydrophilic cellulose sponges using surfactant and pore-foaming agent is demonstrated.
33255011	6	13	contain	has	837:839	arg1	sponge					830:835	the chitosan/cellulose composite sponge	797:835	the chitosan/cellulose composite sponge	797:835	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
33255011	6	13	contain	has	837:839	arg2	ability					860:866	better coagulation ability	841:866	better coagulation ability	841:866	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
33255011	4	14	theme	composite	556:564	arg1	sponge					566:571	the chitosan/cellulose composite sponge	533:571	the chitosan/cellulose composite sponge	533:571	The introduction of chitosan endows the chitosan/cellulose composite sponge with good mechanical properties.
33255011	8	15	theme	hemocompatibility	1106:1122	arg1	results					1095:1101	the results	1091:1101	the results of hemocompatibility and cytotoxicity	1091:1139	Good biocompatibility of the composite sponge is proved by the results of hemocompatibility and cytotoxicity, indicating an excellent candidate as a rapid hemostatic material.
33255011	3	16	theme	high	434:437	arg1	capacity					456:463	high water absorption capacity	434:463	high water absorption capacity	434:463	The obtained cellulose sponges exhibit high water absorption capacity and rapid shape recoverability.
33255011	1	17	theme	potential	193:201	arg1	application					203:213	the potential application	189:213	the potential application of functional cellulose sponges	189:245	Developing a facile and scalable synthetic route is important to explore the potential application of functional cellulose sponges.
33255011	0	18	theme	cellulose/chitosan	24:41	arg1	sponge					43:48	Morphology-controllable cellulose/chitosan sponge	0:48	Morphology-controllable cellulose/chitosan sponge for deep wound hemostasis with surfactant and pore-foaming agent.	0:114	Morphology-controllable cellulose/chitosan sponge for deep wound hemostasis with surfactant and pore-foaming agent.
33255011	4	19	theme	chitosan/cellulose	537:554	arg1	sponge					566:571	the chitosan/cellulose composite sponge	533:571	the chitosan/cellulose composite sponge	533:571	The introduction of chitosan endows the chitosan/cellulose composite sponge with good mechanical properties.
33255011	8	20	theme	rapid	1181:1185	arg1	material					1198:1205	a rapid hemostatic material	1179:1205	a rapid hemostatic material	1179:1205	Good biocompatibility of the composite sponge is proved by the results of hemocompatibility and cytotoxicity, indicating an excellent candidate as a rapid hemostatic material.
33255011	6	21	theme	traditional	882:892	arg1	gauze					894:898	traditional gauze	882:898	traditional gauze	882:898	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
33255011	2	22	theme	surfactant	344:353	arg1	agent					372:376	surfactant and pore-foaming agent	344:376	surfactant and pore-foaming agent	344:376	Here, a simple and efficient strategy to produce porous and hydrophilic cellulose sponges using surfactant and pore-foaming agent is demonstrated.
33255011	6	23	theme	time	777:780	arg1	result					734:739	the result	730:739	the result of the dynamic whole blood clotting time	730:780	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
33255011	3	24	theme	rapid	469:473	arg1	recoverability					481:494	rapid shape recoverability	469:494	rapid shape recoverability	469:494	The obtained cellulose sponges exhibit high water absorption capacity and rapid shape recoverability.
33255011	0	25	theme	Morphology-controllable	0:22	arg1	sponge					43:48	Morphology-controllable cellulose/chitosan sponge	0:48	Morphology-controllable cellulose/chitosan sponge for deep wound hemostasis with surfactant and pore-foaming agent.	0:114	Morphology-controllable cellulose/chitosan sponge for deep wound hemostasis with surfactant and pore-foaming agent.
33255011	8	26	theme	cytotoxicity	1128:1139	arg1	results					1095:1101	the results	1091:1101	the results of hemocompatibility and cytotoxicity	1091:1139	Good biocompatibility of the composite sponge is proved by the results of hemocompatibility and cytotoxicity, indicating an excellent candidate as a rapid hemostatic material.
33255011	1	27	theme	Developing	116:125	arg1	important					168:176	important	168:176	important	168:176	Developing a facile and scalable synthetic route is important to explore the potential application of functional cellulose sponges.
33255011	1	27	theme	Developing	116:125	arg1	route					159:163	a facile and scalable synthetic route	127:163	Developing a facile and scalable synthetic route	116:163	Developing a facile and scalable synthetic route is important to explore the potential application of functional cellulose sponges.
33255011	6	28	theme	clotting	768:775	arg1	time					777:780	the dynamic whole blood clotting time	744:780	the dynamic whole blood clotting time	744:780	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
33255011	2	29	dep	strategy	277:284	arg1	produce					289:295	produce	289:295	to produce porous and hydrophilic cellulose sponges using surfactant and pore-foaming agent	286:376	Here, a simple and efficient strategy to produce porous and hydrophilic cellulose sponges using surfactant and pore-foaming agent is demonstrated.
33255011	3	30	theme	cellulose	408:416	arg1	sponges					418:424	The obtained cellulose sponges	395:424	The obtained cellulose sponges	395:424	The obtained cellulose sponges exhibit high water absorption capacity and rapid shape recoverability.
33255011	6	31	theme	blood	762:766	arg1	time					777:780	the dynamic whole blood clotting time	744:780	the dynamic whole blood clotting time	744:780	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
33255011	1	32	theme	functional	218:227	arg1	sponges					239:245	functional cellulose sponges	218:245	functional cellulose sponges	218:245	Developing a facile and scalable synthetic route is important to explore the potential application of functional cellulose sponges.
33255011	8	33	theme	hemostatic	1187:1196	arg1	material					1198:1205	a rapid hemostatic material	1179:1205	a rapid hemostatic material	1179:1205	Good biocompatibility of the composite sponge is proved by the results of hemocompatibility and cytotoxicity, indicating an excellent candidate as a rapid hemostatic material.
33255011	2	34	theme	cellulose	320:328	arg1	sponges					330:336	porous and hydrophilic cellulose sponges	297:336	porous and hydrophilic cellulose sponges using surfactant and pore-foaming agent	297:376	Here, a simple and efficient strategy to produce porous and hydrophilic cellulose sponges using surfactant and pore-foaming agent is demonstrated.
33255011	6	35	theme	whole	756:760	arg1	time					777:780	the dynamic whole blood clotting time	744:780	the dynamic whole blood clotting time	744:780	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
33255011	1	36	theme	cellulose	229:237	arg1	sponges					239:245	functional cellulose sponges	218:245	functional cellulose sponges	218:245	Developing a facile and scalable synthetic route is important to explore the potential application of functional cellulose sponges.
33255011	0	37	theme	wound	59:63	arg1	hemostasis					65:74	deep wound hemostasis	54:74	deep wound hemostasis	54:74	Morphology-controllable cellulose/chitosan sponge for deep wound hemostasis with surfactant and pore-foaming agent.
33255011	2	38	theme	simple	256:261	arg1	strategy					277:284	a simple and efficient strategy	254:284	a simple and efficient strategy to produce porous and hydrophilic cellulose sponges using surfactant and pore-foaming agent	254:376	Here, a simple and efficient strategy to produce porous and hydrophilic cellulose sponges using surfactant and pore-foaming agent is demonstrated.
33255011	3	39	theme	shape	475:479	arg1	recoverability					481:494	rapid shape recoverability	469:494	rapid shape recoverability	469:494	The obtained cellulose sponges exhibit high water absorption capacity and rapid shape recoverability.
33255011	1	40	theme	sponges	239:245	arg1	application					203:213	the potential application	189:213	the potential application of functional cellulose sponges	189:245	Developing a facile and scalable synthetic route is important to explore the potential application of functional cellulose sponges.
33255011	0	41	theme	deep	54:57	arg1	hemostasis					65:74	deep wound hemostasis	54:74	deep wound hemostasis	54:74	Morphology-controllable cellulose/chitosan sponge for deep wound hemostasis with surfactant and pore-foaming agent.
33255011	4	42	theme	chitosan	517:524	arg1	introduction					501:512	The introduction	497:512	The introduction of chitosan	497:524	The introduction of chitosan endows the chitosan/cellulose composite sponge with good mechanical properties.
33255011	8	43	theme	Good	1032:1035	arg1	biocompatibility					1037:1052	Good biocompatibility	1032:1052	Good biocompatibility of the composite sponge	1032:1076	Good biocompatibility of the composite sponge is proved by the results of hemocompatibility and cytotoxicity, indicating an excellent candidate as a rapid hemostatic material.
33255011	3	44	theme	water	439:443	arg1	capacity					456:463	high water absorption capacity	434:463	high water absorption capacity	434:463	The obtained cellulose sponges exhibit high water absorption capacity and rapid shape recoverability.
33255011	0	45	with	sponge	43:48	arg1	pore-foaming					96:107	pore-foaming	96:107	pore-foaming	96:107	Morphology-controllable cellulose/chitosan sponge for deep wound hemostasis with surfactant and pore-foaming agent.
33255011	0	45	with	sponge	43:48	arg1	surfactant					81:90	surfactant	81:90	surfactant	81:90	Morphology-controllable cellulose/chitosan sponge for deep wound hemostasis with surfactant and pore-foaming agent.
33255011	3	46	theme	absorption	445:454	arg1	capacity					456:463	high water absorption capacity	434:463	high water absorption capacity	434:463	The obtained cellulose sponges exhibit high water absorption capacity and rapid shape recoverability.
33255011	6	47	theme	coagulation	848:858	arg1	ability					860:866	better coagulation ability	841:866	better coagulation ability	841:866	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
33255011	1	48	theme	scalable	140:147	arg1	important					168:176	important	168:176	important	168:176	Developing a facile and scalable synthetic route is important to explore the potential application of functional cellulose sponges.
33255011	1	48	theme	scalable	140:147	arg1	route					159:163	a facile and scalable synthetic route	127:163	Developing a facile and scalable synthetic route	116:163	Developing a facile and scalable synthetic route is important to explore the potential application of functional cellulose sponges.
33255011	7	49	theme	composite	1014:1022	arg1	sponge					1024:1029	the composite sponge	1010:1029	the composite sponge	1010:1029	Animal experiment further showed that rapid hemostasis within 105 s could be reached with the composite sponge.
33255011	1	50	theme	facile	129:134	arg1	important					168:176	important	168:176	important	168:176	Developing a facile and scalable synthetic route is important to explore the potential application of functional cellulose sponges.
33255011	1	50	theme	facile	129:134	arg1	route					159:163	a facile and scalable synthetic route	127:163	Developing a facile and scalable synthetic route	116:163	Developing a facile and scalable synthetic route is important to explore the potential application of functional cellulose sponges.
33255011	6	51	theme	better	841:846	arg1	ability					860:866	better coagulation ability	841:866	better coagulation ability	841:866	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
33255011	1	52	theme	synthetic	149:157	arg1	important					168:176	important	168:176	important	168:176	Developing a facile and scalable synthetic route is important to explore the potential application of functional cellulose sponges.
33255011	1	52	theme	synthetic	149:157	arg1	route					159:163	a facile and scalable synthetic route	127:163	Developing a facile and scalable synthetic route	116:163	Developing a facile and scalable synthetic route is important to explore the potential application of functional cellulose sponges.
33255011	6	53	theme	composite	820:828	arg1	sponge					830:835	the chitosan/cellulose composite sponge	797:835	the chitosan/cellulose composite sponge	797:835	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
33255011	8	54	theme	composite	1061:1069	arg1	sponge					1071:1076	the composite sponge	1057:1076	the composite sponge	1057:1076	Good biocompatibility of the composite sponge is proved by the results of hemocompatibility and cytotoxicity, indicating an excellent candidate as a rapid hemostatic material.
33255011	2	55	theme	pore-foaming	359:370	arg1	agent					372:376	surfactant and pore-foaming agent	344:376	surfactant and pore-foaming agent	344:376	Here, a simple and efficient strategy to produce porous and hydrophilic cellulose sponges using surfactant and pore-foaming agent is demonstrated.
33255011	2	56	theme	efficient	267:275	arg1	strategy					277:284	a simple and efficient strategy	254:284	a simple and efficient strategy to produce porous and hydrophilic cellulose sponges using surfactant and pore-foaming agent	254:376	Here, a simple and efficient strategy to produce porous and hydrophilic cellulose sponges using surfactant and pore-foaming agent is demonstrated.
33255011	5	57	from	effects	617:623	arg1	aeruginosa					685:694	Pseudomonas aeruginosa	673:694	Pseudomonas aeruginosa	673:694	Inhibitory effects on Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa are particularly proved.
33255011	5	57	from	effects	617:623	arg1	coli					640:643	Escherichia coli	628:643	Escherichia coli	628:643	Inhibitory effects on Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa are particularly proved.
33255011	5	57	from	effects	617:623	arg1	aureus					661:666	Staphylococcus aureus	646:666	Staphylococcus aureus	646:666	Inhibitory effects on Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa are particularly proved.
33255011	5	58	theme	Inhibitory	606:615	arg1	effects					617:623	Inhibitory effects	606:623	Inhibitory effects on Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa	606:694	Inhibitory effects on Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa are particularly proved.
32251752	1	0	theme	ε-polycaprolactone	366:383	arg1	matrix					329:334	the blend matrix	319:334	the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold	319:410	Laser ablation technique was utilized to synthesize versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold.
32251752	5	1	theme	morphological	945:957	arg1	investigations					959:972	The morphological investigations	941:972	The morphological investigations	941:972	The morphological investigations indicated the scaffolds were configured in a non-oriented form with diameters 0.45-0.9 μm in the case of ZnONPs involved in blend matrix fibers.
32251752	5	2	from	0.45-0.9 μm	1052:1062	arg1	case					1071:1074	the case	1067:1074	the case of ZnONPs involved in blend matrix fibers	1067:1116	The morphological investigations indicated the scaffolds were configured in a non-oriented form with diameters 0.45-0.9 μm in the case of ZnONPs involved in blend matrix fibers.
32251752	7	3	theme	Bio-blend	1329:1337	arg1	matrices					1348:1355	Bio-blend polymers matrices	1329:1355	Bio-blend polymers matrices carrying nanoparticles	1329:1378	Bio-blend polymers matrices carrying nanoparticles could be tailored for a plethora of biomedical applications upon their compositions.
32251752	7	4	theme	applications	1427:1438	arg1	plethora					1404:1411	a plethora	1402:1411	a plethora of biomedical applications upon their compositions	1402:1462	Bio-blend polymers matrices carrying nanoparticles could be tailored for a plethora of biomedical applications upon their compositions.
32251752	2	5	theme	Electron	650:657	arg1	Microscope					659:668	Field Emission-Scanning Electron Microscope	626:668	Field Emission-Scanning Electron Microscope (FESEM)	626:676	The compositional, microstructural and morphological behaviors for the obtained nanofibers were investigated using X-ray diffraction (XRD), Fourier Transformed Infrared, Transmission Electron Microscope (TEM) and Field Emission-Scanning Electron Microscope (FESEM).
32251752	2	5	theme	Electron	650:657	arg1	FESEM					671:675	FESEM	671:675	FESEM	671:675	The compositional, microstructural and morphological behaviors for the obtained nanofibers were investigated using X-ray diffraction (XRD), Fourier Transformed Infrared, Transmission Electron Microscope (TEM) and Field Emission-Scanning Electron Microscope (FESEM).
32251752	0	6	contain	containing	81:90	arg1	acetate/ε-polycaprolactone					54:79	cellulose acetate/ε-polycaprolactone	44:79	cellulose acetate/ε-polycaprolactone containing metallic nanoparticles prepared by laser ablation for wound disinfection applications	44:176	Blend biopolymeric nanofibrous scaffolds of cellulose acetate/ε-polycaprolactone containing metallic nanoparticles prepared by laser ablation for wound disinfection applications.
32251752	0	6	contain	containing	81:90	arg2	nanoparticles					101:113	metallic nanoparticles	92:113	metallic nanoparticles prepared by laser ablation for wound disinfection applications	92:176	Blend biopolymeric nanofibrous scaffolds of cellulose acetate/ε-polycaprolactone containing metallic nanoparticles prepared by laser ablation for wound disinfection applications.
32251752	1	7	used	utilized	208:215	arg2	technique					194:202	Laser ablation technique	179:202	Laser ablation technique	179:202	Laser ablation technique was utilized to synthesize versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold.
32251752	6	8	theme	antibacterial	1201:1213	arg1	activity					1215:1222	the antibacterial activity	1197:1222	the antibacterial activity of both AgNPs and CuONPs	1197:1247	The ratio of viable cells displays that compositions are biocompatible, while the antibacterial activity of both AgNPs and CuONPs showed an inhibition zone around 11.2.3 ± 2.2 mm and 9.4 ± 1.2 mm respectively.
32251752	0	9	theme	laser	127:131	arg1	ablation					133:140	laser ablation	127:140	laser ablation	127:140	Blend biopolymeric nanofibrous scaffolds of cellulose acetate/ε-polycaprolactone containing metallic nanoparticles prepared by laser ablation for wound disinfection applications.
32251752	2	10	theme	Emission-Scanning	632:648	arg1	Microscope					659:668	Field Emission-Scanning Electron Microscope	626:668	Field Emission-Scanning Electron Microscope (FESEM)	626:676	The compositional, microstructural and morphological behaviors for the obtained nanofibers were investigated using X-ray diffraction (XRD), Fourier Transformed Infrared, Transmission Electron Microscope (TEM) and Field Emission-Scanning Electron Microscope (FESEM).
32251752	2	10	theme	Emission-Scanning	632:648	arg1	FESEM					671:675	FESEM	671:675	FESEM	671:675	The compositional, microstructural and morphological behaviors for the obtained nanofibers were investigated using X-ray diffraction (XRD), Fourier Transformed Infrared, Transmission Electron Microscope (TEM) and Field Emission-Scanning Electron Microscope (FESEM).
32251752	6	11	theme	CuONPs	1242:1247	arg1	activity					1215:1222	the antibacterial activity	1197:1222	the antibacterial activity of both AgNPs and CuONPs	1197:1247	The ratio of viable cells displays that compositions are biocompatible, while the antibacterial activity of both AgNPs and CuONPs showed an inhibition zone around 11.2.3 ± 2.2 mm and 9.4 ± 1.2 mm respectively.
32251752	5	12	from	case	1071:1074	arg1	0.45-0.9 μm					1052:1062	0.45-0.9 μm	1052:1062	0.45-0.9 μm	1052:1062	The morphological investigations indicated the scaffolds were configured in a non-oriented form with diameters 0.45-0.9 μm in the case of ZnONPs involved in blend matrix fibers.
32251752	0	13	theme	wound	146:150	arg1	applications					165:176	wound disinfection applications	146:176	wound disinfection applications	146:176	Blend biopolymeric nanofibrous scaffolds of cellulose acetate/ε-polycaprolactone containing metallic nanoparticles prepared by laser ablation for wound disinfection applications.
32251752	1	14	theme	nanofibrous	391:401	arg1	scaffold					403:410	nanofibrous scaffold	391:410	nanofibrous scaffold	391:410	Laser ablation technique was utilized to synthesize versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold.
32251752	4	15	theme	CA/PCL	887:892	arg1	scaffold					902:909	CA/PCL fibrous scaffold	887:909	CA/PCL fibrous scaffold with diameters around 160 nm	887:938	TEM micrographs refer that while CuONPs were involved in the middle of CA/PCL fibrous scaffold with diameters around 160 nm.
32251752	6	16	theme	cells	1139:1143	arg1	ratio					1123:1127	The ratio	1119:1127	The ratio of viable cells	1119:1143	The ratio of viable cells displays that compositions are biocompatible, while the antibacterial activity of both AgNPs and CuONPs showed an inhibition zone around 11.2.3 ± 2.2 mm and 9.4 ± 1.2 mm respectively.
32251752	2	17	theme	compositional	417:429	arg1	behaviors					466:474	The compositional, microstructural and morphological behaviors	413:474	The compositional, microstructural and morphological behaviors for the obtained nanofibers	413:502	The compositional, microstructural and morphological behaviors for the obtained nanofibers were investigated using X-ray diffraction (XRD), Fourier Transformed Infrared, Transmission Electron Microscope (TEM) and Field Emission-Scanning Electron Microscope (FESEM).
32251752	2	18	theme	microstructural	432:446	arg1	behaviors					466:474	The compositional, microstructural and morphological behaviors	413:474	The compositional, microstructural and morphological behaviors for the obtained nanofibers	413:502	The compositional, microstructural and morphological behaviors for the obtained nanofibers were investigated using X-ray diffraction (XRD), Fourier Transformed Infrared, Transmission Electron Microscope (TEM) and Field Emission-Scanning Electron Microscope (FESEM).
32251752	2	19	theme	Fourier	553:559	arg1	Infrared					573:580	Fourier Transformed Infrared	553:580	Fourier Transformed Infrared	553:580	The compositional, microstructural and morphological behaviors for the obtained nanofibers were investigated using X-ray diffraction (XRD), Fourier Transformed Infrared, Transmission Electron Microscope (TEM) and Field Emission-Scanning Electron Microscope (FESEM).
32251752	1	20	dep	Acetate	349:355	arg1	scaffold					403:410	nanofibrous scaffold	391:410	nanofibrous scaffold	391:410	Laser ablation technique was utilized to synthesize versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold.
32251752	5	21	theme	0.45-0.9 μm	1052:1062	arg1	diameters					1042:1050	diameters	1042:1050	diameters 0.45-0.9 μm in the case of ZnONPs involved in blend matrix fibers	1042:1116	The morphological investigations indicated the scaffolds were configured in a non-oriented form with diameters 0.45-0.9 μm in the case of ZnONPs involved in blend matrix fibers.
32251752	2	22	theme	Transformed	561:571	arg1	Infrared					573:580	Fourier Transformed Infrared	553:580	Fourier Transformed Infrared	553:580	The compositional, microstructural and morphological behaviors for the obtained nanofibers were investigated using X-ray diffraction (XRD), Fourier Transformed Infrared, Transmission Electron Microscope (TEM) and Field Emission-Scanning Electron Microscope (FESEM).
32251752	0	23	theme	biopolymeric	6:17	arg1	scaffolds					31:39	Blend biopolymeric nanofibrous scaffolds	0:39	Blend biopolymeric nanofibrous scaffolds of cellulose acetate/ε-polycaprolactone containing metallic nanoparticles prepared by laser ablation for wound disinfection applications.	0:177	Blend biopolymeric nanofibrous scaffolds of cellulose acetate/ε-polycaprolactone containing metallic nanoparticles prepared by laser ablation for wound disinfection applications.
32251752	4	24	theme	scaffold	902:909	arg1	middle					877:882	the middle	873:882	the middle of CA/PCL fibrous scaffold with diameters around 160 nm	873:938	TEM micrographs refer that while CuONPs were involved in the middle of CA/PCL fibrous scaffold with diameters around 160 nm.
32251752	7	25	contain	carrying	1357:1364	arg2	nanoparticles					1366:1378	nanoparticles	1366:1378	nanoparticles	1366:1378	Bio-blend polymers matrices carrying nanoparticles could be tailored for a plethora of biomedical applications upon their compositions.
32251752	7	25	contain	carrying	1357:1364	arg1	matrices					1348:1355	Bio-blend polymers matrices	1329:1355	Bio-blend polymers matrices carrying nanoparticles	1329:1378	Bio-blend polymers matrices carrying nanoparticles could be tailored for a plethora of biomedical applications upon their compositions.
32251752	1	26	theme	versatile	231:239	arg1	CuO					286:288	CuO	286:288	CuO	286:288	Laser ablation technique was utilized to synthesize versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold.
32251752	1	26	theme	versatile	231:239	arg1	Ag					279:280	Ag	279:280	Ag	279:280	Laser ablation technique was utilized to synthesize versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold.
32251752	1	26	theme	versatile	231:239	arg1	ZnO					274:276	ZnO	274:276	ZnO	274:276	Laser ablation technique was utilized to synthesize versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold.
32251752	1	26	theme	versatile	231:239	arg1	nanoparticles					250:262	versatile metallic nanoparticles	231:262	versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold	231:410	Laser ablation technique was utilized to synthesize versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold.
32251752	0	27	theme	Blend	0:4	arg1	scaffolds					31:39	Blend biopolymeric nanofibrous scaffolds	0:39	Blend biopolymeric nanofibrous scaffolds of cellulose acetate/ε-polycaprolactone containing metallic nanoparticles prepared by laser ablation for wound disinfection applications.	0:177	Blend biopolymeric nanofibrous scaffolds of cellulose acetate/ε-polycaprolactone containing metallic nanoparticles prepared by laser ablation for wound disinfection applications.
32251752	1	28	theme	blend	323:327	arg1	matrix					329:334	the blend matrix	319:334	the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold	319:410	Laser ablation technique was utilized to synthesize versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold.
32251752	4	29	theme	fibrous	894:900	arg1	scaffold					902:909	CA/PCL fibrous scaffold	887:909	CA/PCL fibrous scaffold with diameters around 160 nm	887:938	TEM micrographs refer that while CuONPs were involved in the middle of CA/PCL fibrous scaffold with diameters around 160 nm.
32251752	1	30	theme	metallic	241:248	arg1	CuO					286:288	CuO	286:288	CuO	286:288	Laser ablation technique was utilized to synthesize versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold.
32251752	1	30	theme	metallic	241:248	arg1	Ag					279:280	Ag	279:280	Ag	279:280	Laser ablation technique was utilized to synthesize versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold.
32251752	1	30	theme	metallic	241:248	arg1	ZnO					274:276	ZnO	274:276	ZnO	274:276	Laser ablation technique was utilized to synthesize versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold.
32251752	1	30	theme	metallic	241:248	arg1	nanoparticles					250:262	versatile metallic nanoparticles	231:262	versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold	231:410	Laser ablation technique was utilized to synthesize versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold.
32251752	2	31	theme	obtained	484:491	arg1	nanofibers					493:502	the obtained nanofibers	480:502	the obtained nanofibers	480:502	The compositional, microstructural and morphological behaviors for the obtained nanofibers were investigated using X-ray diffraction (XRD), Fourier Transformed Infrared, Transmission Electron Microscope (TEM) and Field Emission-Scanning Electron Microscope (FESEM).
32251752	3	32	dep	aureus	766:771	arg1	aureus					777:782	S. aureus	774:782	S. aureus	774:782	The cell viability and antibacterial activity were investigated against Staphylococcus aureus (S. aureus) and Escherichia coli (E-coli).
32251752	0	33	theme	nanofibrous	19:29	arg1	scaffolds					31:39	Blend biopolymeric nanofibrous scaffolds	0:39	Blend biopolymeric nanofibrous scaffolds of cellulose acetate/ε-polycaprolactone containing metallic nanoparticles prepared by laser ablation for wound disinfection applications.	0:177	Blend biopolymeric nanofibrous scaffolds of cellulose acetate/ε-polycaprolactone containing metallic nanoparticles prepared by laser ablation for wound disinfection applications.
32251752	0	34	theme	disinfection	152:163	arg1	applications					165:176	wound disinfection applications	146:176	wound disinfection applications	146:176	Blend biopolymeric nanofibrous scaffolds of cellulose acetate/ε-polycaprolactone containing metallic nanoparticles prepared by laser ablation for wound disinfection applications.
32251752	5	35	theme	non-oriented	1019:1030	arg1	form					1032:1035	a non-oriented form	1017:1035	a non-oriented form with diameters 0.45-0.9 μm in the case of ZnONPs involved in blend matrix fibers	1017:1116	The morphological investigations indicated the scaffolds were configured in a non-oriented form with diameters 0.45-0.9 μm in the case of ZnONPs involved in blend matrix fibers.
32251752	7	36	theme	polymers	1339:1346	arg1	matrices					1348:1355	Bio-blend polymers matrices	1329:1355	Bio-blend polymers matrices carrying nanoparticles	1329:1378	Bio-blend polymers matrices carrying nanoparticles could be tailored for a plethora of biomedical applications upon their compositions.
32251752	0	37	theme	cellulose	44:52	arg1	acetate/ε-polycaprolactone					54:79	cellulose acetate/ε-polycaprolactone	44:79	cellulose acetate/ε-polycaprolactone containing metallic nanoparticles prepared by laser ablation for wound disinfection applications	44:176	Blend biopolymeric nanofibrous scaffolds of cellulose acetate/ε-polycaprolactone containing metallic nanoparticles prepared by laser ablation for wound disinfection applications.
32251752	1	38	theme	cellulose	339:347	arg1	CA					358:359	CA	358:359	CA	358:359	Laser ablation technique was utilized to synthesize versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold.
32251752	1	38	theme	cellulose	339:347	arg1	Acetate					349:355	cellulose Acetate	339:355	cellulose Acetate (CA)	339:360	Laser ablation technique was utilized to synthesize versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold.
32251752	2	39	theme	Electron	596:603	arg1	Microscope					605:614	Transmission Electron Microscope	583:614	Transmission Electron Microscope (TEM)	583:620	The compositional, microstructural and morphological behaviors for the obtained nanofibers were investigated using X-ray diffraction (XRD), Fourier Transformed Infrared, Transmission Electron Microscope (TEM) and Field Emission-Scanning Electron Microscope (FESEM).
32251752	2	39	theme	Electron	596:603	arg1	TEM					617:619	TEM	617:619	TEM	617:619	The compositional, microstructural and morphological behaviors for the obtained nanofibers were investigated using X-ray diffraction (XRD), Fourier Transformed Infrared, Transmission Electron Microscope (TEM) and Field Emission-Scanning Electron Microscope (FESEM).
32251752	3	40	theme	antibacterial	702:714	arg1	activity					716:723	antibacterial activity	702:723	antibacterial activity	702:723	The cell viability and antibacterial activity were investigated against Staphylococcus aureus (S. aureus) and Escherichia coli (E-coli).
32251752	5	41	theme	ZnONPs	1079:1084	arg1	case					1071:1074	the case	1067:1074	the case of ZnONPs involved in blend matrix fibers	1067:1116	The morphological investigations indicated the scaffolds were configured in a non-oriented form with diameters 0.45-0.9 μm in the case of ZnONPs involved in blend matrix fibers.
32251752	7	42	theme	biomedical	1416:1425	arg1	applications					1427:1438	biomedical applications	1416:1438	biomedical applications	1416:1438	Bio-blend polymers matrices carrying nanoparticles could be tailored for a plethora of biomedical applications upon their compositions.
32251752	2	43	theme	morphological	452:464	arg1	behaviors					466:474	The compositional, microstructural and morphological behaviors	413:474	The compositional, microstructural and morphological behaviors for the obtained nanofibers	413:502	The compositional, microstructural and morphological behaviors for the obtained nanofibers were investigated using X-ray diffraction (XRD), Fourier Transformed Infrared, Transmission Electron Microscope (TEM) and Field Emission-Scanning Electron Microscope (FESEM).
32251752	2	44	theme	X-ray	528:532	arg1	XRD					547:549	XRD	547:549	XRD	547:549	The compositional, microstructural and morphological behaviors for the obtained nanofibers were investigated using X-ray diffraction (XRD), Fourier Transformed Infrared, Transmission Electron Microscope (TEM) and Field Emission-Scanning Electron Microscope (FESEM).
32251752	2	44	theme	X-ray	528:532	arg1	diffraction					534:544	X-ray diffraction	528:544	X-ray diffraction (XRD)	528:550	The compositional, microstructural and morphological behaviors for the obtained nanofibers were investigated using X-ray diffraction (XRD), Fourier Transformed Infrared, Transmission Electron Microscope (TEM) and Field Emission-Scanning Electron Microscope (FESEM).
32251752	1	45	theme	Acetate	349:355	arg1	matrix					329:334	the blend matrix	319:334	the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold	319:410	Laser ablation technique was utilized to synthesize versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold.
32251752	2	46	theme	Transmission	583:594	arg1	Microscope					605:614	Transmission Electron Microscope	583:614	Transmission Electron Microscope (TEM)	583:620	The compositional, microstructural and morphological behaviors for the obtained nanofibers were investigated using X-ray diffraction (XRD), Fourier Transformed Infrared, Transmission Electron Microscope (TEM) and Field Emission-Scanning Electron Microscope (FESEM).
32251752	2	46	theme	Transmission	583:594	arg1	TEM					617:619	TEM	617:619	TEM	617:619	The compositional, microstructural and morphological behaviors for the obtained nanofibers were investigated using X-ray diffraction (XRD), Fourier Transformed Infrared, Transmission Electron Microscope (TEM) and Field Emission-Scanning Electron Microscope (FESEM).
32251752	3	47	theme	cell	683:686	arg1	viability					688:696	The cell viability	679:696	The cell viability	679:696	The cell viability and antibacterial activity were investigated against Staphylococcus aureus (S. aureus) and Escherichia coli (E-coli).
32251752	5	48	with	form	1032:1035	arg1	diameters					1042:1050	diameters	1042:1050	diameters 0.45-0.9 μm in the case of ZnONPs involved in blend matrix fibers	1042:1116	The morphological investigations indicated the scaffolds were configured in a non-oriented form with diameters 0.45-0.9 μm in the case of ZnONPs involved in blend matrix fibers.
32251752	2	49	theme	Field	626:630	arg1	Microscope					659:668	Field Emission-Scanning Electron Microscope	626:668	Field Emission-Scanning Electron Microscope (FESEM)	626:676	The compositional, microstructural and morphological behaviors for the obtained nanofibers were investigated using X-ray diffraction (XRD), Fourier Transformed Infrared, Transmission Electron Microscope (TEM) and Field Emission-Scanning Electron Microscope (FESEM).
32251752	2	49	theme	Field	626:630	arg1	FESEM					671:675	FESEM	671:675	FESEM	671:675	The compositional, microstructural and morphological behaviors for the obtained nanofibers were investigated using X-ray diffraction (XRD), Fourier Transformed Infrared, Transmission Electron Microscope (TEM) and Field Emission-Scanning Electron Microscope (FESEM).
32251752	0	50	theme	acetate/ε-polycaprolactone	54:79	arg1	scaffolds					31:39	Blend biopolymeric nanofibrous scaffolds	0:39	Blend biopolymeric nanofibrous scaffolds of cellulose acetate/ε-polycaprolactone containing metallic nanoparticles prepared by laser ablation for wound disinfection applications.	0:177	Blend biopolymeric nanofibrous scaffolds of cellulose acetate/ε-polycaprolactone containing metallic nanoparticles prepared by laser ablation for wound disinfection applications.
32251752	5	51	theme	matrix	1104:1109	arg1	fibers					1111:1116	blend matrix fibers	1098:1116	blend matrix fibers	1098:1116	The morphological investigations indicated the scaffolds were configured in a non-oriented form with diameters 0.45-0.9 μm in the case of ZnONPs involved in blend matrix fibers.
32251752	5	52	theme	blend	1098:1102	arg1	fibers					1111:1116	blend matrix fibers	1098:1116	blend matrix fibers	1098:1116	The morphological investigations indicated the scaffolds were configured in a non-oriented form with diameters 0.45-0.9 μm in the case of ZnONPs involved in blend matrix fibers.
32251752	6	53	theme	AgNPs	1232:1236	arg1	activity					1215:1222	the antibacterial activity	1197:1222	the antibacterial activity of both AgNPs and CuONPs	1197:1247	The ratio of viable cells displays that compositions are biocompatible, while the antibacterial activity of both AgNPs and CuONPs showed an inhibition zone around 11.2.3 ± 2.2 mm and 9.4 ± 1.2 mm respectively.
32251752	4	54	theme	TEM	816:818	arg1	micrographs					820:830	TEM micrographs	816:830	TEM micrographs	816:830	TEM micrographs refer that while CuONPs were involved in the middle of CA/PCL fibrous scaffold with diameters around 160 nm.
32251752	1	55	theme	Laser	179:183	arg1	technique					194:202	Laser ablation technique	179:202	Laser ablation technique	179:202	Laser ablation technique was utilized to synthesize versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold.
32251752	6	56	theme	inhibition	1259:1268	arg1	zone					1270:1273	an inhibition zone	1256:1273	an inhibition zone around 11.2.3 ± 2.2 mm and 9.4 ± 1.2 mm	1256:1313	The ratio of viable cells displays that compositions are biocompatible, while the antibacterial activity of both AgNPs and CuONPs showed an inhibition zone around 11.2.3 ± 2.2 mm and 9.4 ± 1.2 mm respectively.
32251752	1	57	theme	ablation	185:192	arg1	technique					194:202	Laser ablation technique	179:202	Laser ablation technique	179:202	Laser ablation technique was utilized to synthesize versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nanofibrous scaffold.
32251752	6	58	theme	viable	1132:1137	arg1	cells					1139:1143	viable cells	1132:1143	viable cells	1132:1143	The ratio of viable cells displays that compositions are biocompatible, while the antibacterial activity of both AgNPs and CuONPs showed an inhibition zone around 11.2.3 ± 2.2 mm and 9.4 ± 1.2 mm respectively.
32251752	0	59	theme	metallic	92:99	arg1	nanoparticles					101:113	metallic nanoparticles	92:113	metallic nanoparticles prepared by laser ablation for wound disinfection applications	92:176	Blend biopolymeric nanofibrous scaffolds of cellulose acetate/ε-polycaprolactone containing metallic nanoparticles prepared by laser ablation for wound disinfection applications.
32251752	4	60	with	scaffold	902:909	arg1	diameters					916:924	diameters	916:924	diameters around 160 nm	916:938	TEM micrographs refer that while CuONPs were involved in the middle of CA/PCL fibrous scaffold with diameters around 160 nm.
32007549	3	0	theme	composites	369:378	arg1	performance					348:358	performance	348:358	performance	348:358	The microstructures and performance of these composites were investigated by SEM, DMA, XRD, TGA and tensile testing.
32007549	3	0	theme	composites	369:378	arg1	microstructures					328:342	microstructures	328:342	microstructures	328:342	The microstructures and performance of these composites were investigated by SEM, DMA, XRD, TGA and tensile testing.
32007549	7	1	contain	has	1011:1013	arg1	composite					935:943	the hybrid composite	924:943	the hybrid composite containing up to 40% enteromorpha reinforced with nano-clay	924:1003	The results showed that the hybrid composite containing up to 40% enteromorpha reinforced with nano-clay still has similar or even slightly better mechanical properties compared with pure starch-based materials.
32007549	7	1	contain	has	1011:1013	arg2	properties					1058:1067	similar or even slightly better mechanical properties	1015:1067	similar or even slightly better mechanical properties	1015:1067	The results showed that the hybrid composite containing up to 40% enteromorpha reinforced with nano-clay still has similar or even slightly better mechanical properties compared with pure starch-based materials.
32007549	8	2	theme	natural	1149:1155	arg1	components					1122:1131	all components	1118:1131	all components	1118:1131	Since all components are hydrophilic natural materials, the interfaces between them are very good, and the composites are environmentally friendly and biodegradable.
32007549	8	2	theme	natural	1149:1155	arg1	materials					1157:1165	hydrophilic natural materials	1137:1165	hydrophilic natural materials	1137:1165	Since all components are hydrophilic natural materials, the interfaces between them are very good, and the composites are environmentally friendly and biodegradable.
32007549	4	3	theme	mechanical	508:517	arg1	properties					519:528	very weak mechanical properties	498:528	very weak mechanical properties	498:528	Enteromorpha has a hollow tubular thallus structure with very weak mechanical properties, so it is not expected to have the ability to reinforce the starch matrix even though they have very a good interface.
32007549	6	4	theme	ultrasonic	811:820	arg1	treatment					822:830	ultrasonic treatment	811:830	ultrasonic treatment	811:830	Furthermore, the delaminated clay by water and ultrasonic treatment reinforced the mechanical properties of the starch-based materials.
32007549	4	5	theme	starch	590:595	arg1	matrix					597:602	the starch matrix	586:602	the starch matrix	586:602	Enteromorpha has a hollow tubular thallus structure with very weak mechanical properties, so it is not expected to have the ability to reinforce the starch matrix even though they have very a good interface.
32007549	5	6	theme	starch	726:731	arg1	matrix					733:738	the starch matrix	722:738	the starch matrix	722:738	However, the granulated fine particles of enteromorpha can mix well with the starch matrix and reduce weak points.
32007549	5	7	theme	granulated	662:671	arg1	particles					678:686	the granulated fine particles	658:686	the granulated fine particles of enteromorpha	658:702	However, the granulated fine particles of enteromorpha can mix well with the starch matrix and reduce weak points.
32007549	4	8	contain	have	556:559	arg1	it					534:535	it	534:535	it	534:535	Enteromorpha has a hollow tubular thallus structure with very weak mechanical properties, so it is not expected to have the ability to reinforce the starch matrix even though they have very a good interface.
32007549	4	8	contain	have	556:559	arg2	ability					565:571	the ability to reinforce the starch matrix	561:602	the ability to reinforce the starch matrix	561:602	Enteromorpha has a hollow tubular thallus structure with very weak mechanical properties, so it is not expected to have the ability to reinforce the starch matrix even though they have very a good interface.
32007549	2	9	theme	cheaper	220:226	arg1	filler					228:233	cheaper filler	220:233	cheaper filler	220:233	Enteromorpha was used as cheaper filler since it is a waste from marine pollution, while nano-clay acted as a reinforcing agent.
32007549	2	9	theme	cheaper	220:226	arg1	Enteromorpha					195:206	Enteromorpha	195:206	Enteromorpha	195:206	Enteromorpha was used as cheaper filler since it is a waste from marine pollution, while nano-clay acted as a reinforcing agent.
32007549	5	10	theme	fine	673:676	arg1	particles					678:686	the granulated fine particles	658:686	the granulated fine particles of enteromorpha	658:702	However, the granulated fine particles of enteromorpha can mix well with the starch matrix and reduce weak points.
32007549	4	11	with	structure	483:491	arg1	properties					519:528	very weak mechanical properties	498:528	very weak mechanical properties	498:528	Enteromorpha has a hollow tubular thallus structure with very weak mechanical properties, so it is not expected to have the ability to reinforce the starch matrix even though they have very a good interface.
32007549	4	12	theme	weak	503:506	arg1	properties					519:528	very weak mechanical properties	498:528	very weak mechanical properties	498:528	Enteromorpha has a hollow tubular thallus structure with very weak mechanical properties, so it is not expected to have the ability to reinforce the starch matrix even though they have very a good interface.
32007549	2	13	from	pollution	267:275	arg1	it					241:242	it	241:242	it	241:242	Enteromorpha was used as cheaper filler since it is a waste from marine pollution, while nano-clay acted as a reinforcing agent.
32007549	2	13	from	pollution	267:275	arg1	waste					249:253	a waste	247:253	a waste from marine pollution	247:275	Enteromorpha was used as cheaper filler since it is a waste from marine pollution, while nano-clay acted as a reinforcing agent.
32007549	1	14	theme	biodegradable	114:126	arg1	composites					135:144	Environmentally friendly and biodegradable hybrid composites	85:144	composites	135:144	Environmentally friendly and biodegradable hybrid composites of starch/enteromorpha/nano-clay were developed.
32007549	1	14	theme	biodegradable	114:126	arg1	starch/enteromorpha/nano-clay					149:177	starch/enteromorpha/nano-clay	149:177	starch/enteromorpha/nano-clay	149:177	Environmentally friendly and biodegradable hybrid composites of starch/enteromorpha/nano-clay were developed.
32007549	7	15	contain	containing	945:954	arg1	composite					935:943	the hybrid composite	924:943	the hybrid composite containing up to 40% enteromorpha reinforced with nano-clay	924:1003	The results showed that the hybrid composite containing up to 40% enteromorpha reinforced with nano-clay still has similar or even slightly better mechanical properties compared with pure starch-based materials.
32007549	7	15	contain	containing	945:954	arg2	enteromorpha					966:977	up to 40% enteromorpha	956:977	up to 40% enteromorpha reinforced with nano-clay	956:1003	The results showed that the hybrid composite containing up to 40% enteromorpha reinforced with nano-clay still has similar or even slightly better mechanical properties compared with pure starch-based materials.
32007549	6	16	theme	materials	889:897	arg1	properties					858:867	the mechanical properties	843:867	the mechanical properties of the starch-based materials	843:897	Furthermore, the delaminated clay by water and ultrasonic treatment reinforced the mechanical properties of the starch-based materials.
32007549	6	17	theme	delaminated	781:791	arg1	clay					793:796	the delaminated clay	777:796	the delaminated clay by water and ultrasonic treatment	777:830	Furthermore, the delaminated clay by water and ultrasonic treatment reinforced the mechanical properties of the starch-based materials.
32007549	1	18	theme	hybrid	128:133	arg1	composites					135:144	Environmentally friendly and biodegradable hybrid composites	85:144	composites	135:144	Environmentally friendly and biodegradable hybrid composites of starch/enteromorpha/nano-clay were developed.
32007549	1	18	theme	hybrid	128:133	arg1	starch/enteromorpha/nano-clay					149:177	starch/enteromorpha/nano-clay	149:177	starch/enteromorpha/nano-clay	149:177	Environmentally friendly and biodegradable hybrid composites of starch/enteromorpha/nano-clay were developed.
32007549	4	19	contain	has	454:456	arg2	structure					483:491	a hollow tubular thallus structure	458:491	a hollow tubular thallus structure with very weak mechanical properties	458:528	Enteromorpha has a hollow tubular thallus structure with very weak mechanical properties, so it is not expected to have the ability to reinforce the starch matrix even though they have very a good interface.
32007549	4	19	contain	has	454:456	arg1	Enteromorpha					441:452	Enteromorpha	441:452	Enteromorpha	441:452	Enteromorpha has a hollow tubular thallus structure with very weak mechanical properties, so it is not expected to have the ability to reinforce the starch matrix even though they have very a good interface.
32007549	2	20	used	used	212:215	arg2	filler					228:233	cheaper filler	220:233	cheaper filler	220:233	Enteromorpha was used as cheaper filler since it is a waste from marine pollution, while nano-clay acted as a reinforcing agent.
32007549	2	20	used	used	212:215	arg2	Enteromorpha					195:206	Enteromorpha	195:206	Enteromorpha	195:206	Enteromorpha was used as cheaper filler since it is a waste from marine pollution, while nano-clay acted as a reinforcing agent.
32007549	6	21	theme	mechanical	847:856	arg1	properties					858:867	the mechanical properties	843:867	the mechanical properties of the starch-based materials	843:897	Furthermore, the delaminated clay by water and ultrasonic treatment reinforced the mechanical properties of the starch-based materials.
32007549	7	22	theme	starch-based	1088:1099	arg1	materials					1101:1109	pure starch-based materials	1083:1109	pure starch-based materials	1083:1109	The results showed that the hybrid composite containing up to 40% enteromorpha reinforced with nano-clay still has similar or even slightly better mechanical properties compared with pure starch-based materials.
32007549	6	23	theme	starch-based	876:887	arg1	materials					889:897	the starch-based materials	872:897	the starch-based materials	872:897	Furthermore, the delaminated clay by water and ultrasonic treatment reinforced the mechanical properties of the starch-based materials.
32007549	1	24	theme	starch/enteromorpha/nano-clay	149:177	arg1	composites					135:144	Environmentally friendly and biodegradable hybrid composites	85:144	composites	135:144	Environmentally friendly and biodegradable hybrid composites of starch/enteromorpha/nano-clay were developed.
32007549	1	24	theme	starch/enteromorpha/nano-clay	149:177	arg1	friendly					101:108	Environmentally friendly and biodegradable hybrid composites	85:144	friendly	101:108	Environmentally friendly and biodegradable hybrid composites of starch/enteromorpha/nano-clay were developed.
32007549	1	24	theme	starch/enteromorpha/nano-clay	149:177	arg1	starch/enteromorpha/nano-clay					149:177	starch/enteromorpha/nano-clay	149:177	starch/enteromorpha/nano-clay	149:177	Environmentally friendly and biodegradable hybrid composites of starch/enteromorpha/nano-clay were developed.
32007549	4	25	theme	good	633:636	arg1	interface					638:646	very a good interface	626:646	very a good interface	626:646	Enteromorpha has a hollow tubular thallus structure with very weak mechanical properties, so it is not expected to have the ability to reinforce the starch matrix even though they have very a good interface.
32007549	0	26	theme	hybrid	66:71	arg1	composites					73:82	starch/enteromorpha/nano-clay hybrid composites	36:82	starch/enteromorpha/nano-clay hybrid composites	36:82	Preparation and characterization of starch/enteromorpha/nano-clay hybrid composites.
32007549	4	27	theme	thallus	475:481	arg1	structure					483:491	a hollow tubular thallus structure	458:491	a hollow tubular thallus structure with very weak mechanical properties	458:528	Enteromorpha has a hollow tubular thallus structure with very weak mechanical properties, so it is not expected to have the ability to reinforce the starch matrix even though they have very a good interface.
32007549	5	28	with	mix	708:710	arg1	matrix					733:738	the starch matrix	722:738	the starch matrix	722:738	However, the granulated fine particles of enteromorpha can mix well with the starch matrix and reduce weak points.
32007549	0	29	theme	starch/enteromorpha/nano-clay	36:64	arg1	composites					73:82	starch/enteromorpha/nano-clay hybrid composites	36:82	starch/enteromorpha/nano-clay hybrid composites	36:82	Preparation and characterization of starch/enteromorpha/nano-clay hybrid composites.
32007549	7	30	theme	pure	1083:1086	arg1	materials					1101:1109	pure starch-based materials	1083:1109	pure starch-based materials	1083:1109	The results showed that the hybrid composite containing up to 40% enteromorpha reinforced with nano-clay still has similar or even slightly better mechanical properties compared with pure starch-based materials.
32007549	3	31	theme	tensile	424:430	arg1	testing					432:438	tensile testing	424:438	tensile testing	424:438	The microstructures and performance of these composites were investigated by SEM, DMA, XRD, TGA and tensile testing.
32007549	8	32	theme	hydrophilic	1137:1147	arg1	components					1122:1131	all components	1118:1131	all components	1118:1131	Since all components are hydrophilic natural materials, the interfaces between them are very good, and the composites are environmentally friendly and biodegradable.
32007549	8	32	theme	hydrophilic	1137:1147	arg1	materials					1157:1165	hydrophilic natural materials	1137:1165	hydrophilic natural materials	1137:1165	Since all components are hydrophilic natural materials, the interfaces between them are very good, and the composites are environmentally friendly and biodegradable.
32007549	7	33	theme	better	1040:1045	arg1	properties					1058:1067	similar or even slightly better mechanical properties	1015:1067	similar or even slightly better mechanical properties	1015:1067	The results showed that the hybrid composite containing up to 40% enteromorpha reinforced with nano-clay still has similar or even slightly better mechanical properties compared with pure starch-based materials.
32007549	0	34	theme	composites	73:82	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of starch/enteromorpha/nano-clay hybrid composites.
32007549	0	34	theme	composites	73:82	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of starch/enteromorpha/nano-clay hybrid composites.
32007549	2	35	theme	marine	260:265	arg1	pollution					267:275	marine pollution	260:275	marine pollution	260:275	Enteromorpha was used as cheaper filler since it is a waste from marine pollution, while nano-clay acted as a reinforcing agent.
32007549	7	36	theme	similar	1015:1021	arg1	properties					1058:1067	similar or even slightly better mechanical properties	1015:1067	similar or even slightly better mechanical properties	1015:1067	The results showed that the hybrid composite containing up to 40% enteromorpha reinforced with nano-clay still has similar or even slightly better mechanical properties compared with pure starch-based materials.
32007549	7	37	dep	40	962:963	arg1	to					959:960	to	959:960	to	959:960	The results showed that the hybrid composite containing up to 40% enteromorpha reinforced with nano-clay still has similar or even slightly better mechanical properties compared with pure starch-based materials.
32007549	5	38	theme	enteromorpha	691:702	arg1	particles					678:686	the granulated fine particles	658:686	the granulated fine particles of enteromorpha	658:702	However, the granulated fine particles of enteromorpha can mix well with the starch matrix and reduce weak points.
32007549	2	39	theme	reinforcing	305:315	arg1	agent					317:321	a reinforcing agent	303:321	a reinforcing agent	303:321	Enteromorpha was used as cheaper filler since it is a waste from marine pollution, while nano-clay acted as a reinforcing agent.
32007549	2	39	theme	reinforcing	305:315	arg1	nano-clay					284:292	nano-clay	284:292	nano-clay	284:292	Enteromorpha was used as cheaper filler since it is a waste from marine pollution, while nano-clay acted as a reinforcing agent.
32007549	7	40	theme	hybrid	928:933	arg1	composite					935:943	the hybrid composite	924:943	the hybrid composite containing up to 40% enteromorpha reinforced with nano-clay	924:1003	The results showed that the hybrid composite containing up to 40% enteromorpha reinforced with nano-clay still has similar or even slightly better mechanical properties compared with pure starch-based materials.
32007549	7	41	theme	mechanical	1047:1056	arg1	properties					1058:1067	similar or even slightly better mechanical properties	1015:1067	similar or even slightly better mechanical properties	1015:1067	The results showed that the hybrid composite containing up to 40% enteromorpha reinforced with nano-clay still has similar or even slightly better mechanical properties compared with pure starch-based materials.
32007549	4	42	theme	tubular	467:473	arg1	structure					483:491	a hollow tubular thallus structure	458:491	a hollow tubular thallus structure with very weak mechanical properties	458:528	Enteromorpha has a hollow tubular thallus structure with very weak mechanical properties, so it is not expected to have the ability to reinforce the starch matrix even though they have very a good interface.
32007549	1	43	theme	Environmentally	85:99	arg1	friendly					101:108	Environmentally friendly and biodegradable hybrid composites	85:144	friendly	101:108	Environmentally friendly and biodegradable hybrid composites of starch/enteromorpha/nano-clay were developed.
32007549	7	44	dep	enteromorpha	966:977	arg1	%					964:964	%	964:964	%	964:964	The results showed that the hybrid composite containing up to 40% enteromorpha reinforced with nano-clay still has similar or even slightly better mechanical properties compared with pure starch-based materials.
32007549	5	45	theme	weak	751:754	arg1	points					756:761	weak points	751:761	weak points	751:761	However, the granulated fine particles of enteromorpha can mix well with the starch matrix and reduce weak points.
32007549	4	46	theme	hollow	460:465	arg1	structure					483:491	a hollow tubular thallus structure	458:491	a hollow tubular thallus structure with very weak mechanical properties	458:528	Enteromorpha has a hollow tubular thallus structure with very weak mechanical properties, so it is not expected to have the ability to reinforce the starch matrix even though they have very a good interface.
32007549	7	47	dep	%	964:964	arg1	40					962:963	40	962:963	40	962:963	The results showed that the hybrid composite containing up to 40% enteromorpha reinforced with nano-clay still has similar or even slightly better mechanical properties compared with pure starch-based materials.
32007549	4	48	contain	have	621:624	arg2	interface					638:646	very a good interface	626:646	very a good interface	626:646	Enteromorpha has a hollow tubular thallus structure with very weak mechanical properties, so it is not expected to have the ability to reinforce the starch matrix even though they have very a good interface.
32007549	4	48	contain	have	621:624	arg1	they					616:619	they	616:619	they	616:619	Enteromorpha has a hollow tubular thallus structure with very weak mechanical properties, so it is not expected to have the ability to reinforce the starch matrix even though they have very a good interface.
34020453	11	0	dep	was	1811:1813	arg1	-14.93					1899:1904	-14.93	1899:1904	-14.93	1899:1904	Furthermore, there was an interaction between 3'-SL and age for receptive language (adjusted P = 0.03; EF, -14.93; 95% CI, -25.29 to -4.24).
34020453	11	0	dep	was	1811:1813	arg1	P = 0.03					1885:1892	adjusted P = 0.03	1876:1892	adjusted P = 0.03	1876:1892	Furthermore, there was an interaction between 3'-SL and age for receptive language (adjusted P = 0.03; EF, -14.93; 95% CI, -25.29 to -4.24).
34020453	3	1	theme	A-tetra+ and	632:643	arg1	groups					654:659	A-tetra+ and A-tetra- groups	632:659	A-tetra+ and A-tetra- groups in children (<25 months old)	632:688	OBJECTIVES This study aimed to determine whether associations exist between HMO concentrations and cognitive development, and whether the associations vary between A-tetra+ and A-tetra- groups in children (<25 months old).
34020453	1	2	theme	BACKGROUND	95:104	arg1	polymorphisms					114:126	BACKGROUND Genetic polymorphisms	95:126	BACKGROUND Genetic polymorphisms leading to variations in human milk oligosaccharide (HMO) composition	95:196	BACKGROUND Genetic polymorphisms leading to variations in human milk oligosaccharide (HMO) composition have been reported.
34020453	10	3	theme	adjusted	1715:1722	arg1	P = 0.048					1724:1732	adjusted P = 0.048	1715:1732	adjusted P = 0.048	1715:1732	This association was driven by the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores.
34020453	10	3	theme	adjusted	1715:1722	arg1	7.53					1739:1742	7.53	1739:1742	7.53	1739:1742	This association was driven by the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores.
34020453	2	4	with	women	351:355	arg1	A					373:373	blood type A	362:373	blood type A	362:373	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	11	5	theme	adjusted	1876:1883	arg1	-14.93					1899:1904	-14.93	1899:1904	-14.93	1899:1904	Furthermore, there was an interaction between 3'-SL and age for receptive language (adjusted P = 0.03; EF, -14.93; 95% CI, -25.29 to -4.24).
34020453	11	5	theme	adjusted	1876:1883	arg1	P = 0.03					1885:1892	adjusted P = 0.03	1876:1892	adjusted P = 0.03	1876:1892	Furthermore, there was an interaction between 3'-SL and age for receptive language (adjusted P = 0.03; EF, -14.93; 95% CI, -25.29 to -4.24).
34020453	10	6	dep	receptive	1638:1646	arg1	P = 0.015					1658:1666	adjusted P = 0.015	1649:1666	adjusted P = 0.015; EF	1649:1670	This association was driven by the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores.
34020453	10	6	dep	receptive	1638:1646	arg1	P = 0.048					1724:1732	adjusted P = 0.048	1715:1732	adjusted P = 0.048	1715:1732	This association was driven by the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores.
34020453	10	6	dep	receptive	1638:1646	arg1	7.53					1739:1742	7.53	1739:1742	7.53	1739:1742	This association was driven by the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores.
34020453	10	7	theme	expressive	1703:1712	arg1	scores					1784:1789	the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores	1634:1789	the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores	1634:1789	This association was driven by the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores.
34020453	9	8	dep	[P = 0.002	1522:1531	arg1	%					1561:1561	95% CI	1559:1564	95% CI	1559:1564	The composite score and 3'-sialyllactose (3'-SL) levels were positively associated [P = 0.002; effect size (EF), 13.12; 95% CI, 5.36-20.80] in the A-tetra + group.
34020453	9	8	dep	[P = 0.002	1522:1531	arg1	EF					1547:1548	EF	1547:1548	EF	1547:1548	The composite score and 3'-sialyllactose (3'-SL) levels were positively associated [P = 0.002; effect size (EF), 13.12; 95% CI, 5.36-20.80] in the A-tetra + group.
34020453	9	8	dep	[P = 0.002	1522:1531	arg1	13.12					1552:1556	13.12	1552:1556	13.12	1552:1556	The composite score and 3'-sialyllactose (3'-SL) levels were positively associated [P = 0.002; effect size (EF), 13.12; 95% CI, 5.36-20.80] in the A-tetra + group.
34020453	9	8	dep	[P = 0.002	1522:1531	arg1	size					1541:1544	effect size	1534:1544	effect size (EF)	1534:1549	The composite score and 3'-sialyllactose (3'-SL) levels were positively associated [P = 0.002; effect size (EF), 13.12; 95% CI, 5.36-20.80] in the A-tetra + group.
34020453	9	8	dep	[P = 0.002	1522:1531	arg1	5.36-20.80					1567:1576	5.36-20.80	1567:1576	5.36-20.80	1567:1576	The composite score and 3'-sialyllactose (3'-SL) levels were positively associated [P = 0.002; effect size (EF), 13.12; 95% CI, 5.36-20.80] in the A-tetra + group.
34020453	6	9	theme	A-tetra+ or	1052:1062	arg1	levels					1044:1049	A-tetra levels	1036:1049	A-tetra levels (A-tetra+ or A-tetra-)	1036:1072	Linear mixed effects models were employed by stratifying children based on A-tetra levels (A-tetra+ or A-tetra-) to assess associations between age-removed HMO concentrations and both MSEL composite scores and the 5 subdomain scores.
34020453	6	9	theme	A-tetra+ or	1052:1062	arg1	A-tetra-					1064:1071	A-tetra+ or A-tetra-	1052:1071	A-tetra+ or A-tetra-	1052:1071	Linear mixed effects models were employed by stratifying children based on A-tetra levels (A-tetra+ or A-tetra-) to assess associations between age-removed HMO concentrations and both MSEL composite scores and the 5 subdomain scores.
34020453	6	10	theme	composite	1150:1158	arg1	scores					1160:1165	MSEL composite scores	1145:1165	MSEL composite scores	1145:1165	Linear mixed effects models were employed by stratifying children based on A-tetra levels (A-tetra+ or A-tetra-) to assess associations between age-removed HMO concentrations and both MSEL composite scores and the 5 subdomain scores.
34020453	4	11	dep	children	732:739	arg1	months					747:752	2-25 months old	742:756	2-25 months old	742:756	METHODS We enrolled typically developing children (2-25 months old; mean, 10 months old) who were at least partially breastfed at the study visit.
34020453	7	12	theme	A-tetra+	1270:1277	arg1	dyads					1328:1332	66 dyads	1325:1332	66 dyads	1325:1332	RESULTS A total of 99 mother-child dyads and 183 HM samples were included (A-tetra+: 57 samples, 33 dyads; A-tetra-: 126 samples, 66 dyads).
34020453	7	12	theme	A-tetra+	1270:1277	arg1	samples					1283:1289	A-tetra+: 57 samples	1270:1289	A-tetra+: 57 samples	1270:1289	RESULTS A total of 99 mother-child dyads and 183 HM samples were included (A-tetra+: 57 samples, 33 dyads; A-tetra-: 126 samples, 66 dyads).
34020453	7	12	theme	A-tetra+	1270:1277	arg1	dyads					1295:1299	33 dyads	1292:1299	33 dyads	1292:1299	RESULTS A total of 99 mother-child dyads and 183 HM samples were included (A-tetra+: 57 samples, 33 dyads; A-tetra-: 126 samples, 66 dyads).
34020453	11	13	dep	P = 0.03	1885:1892	arg1	-4.24					1925:1929	-4.24	1925:1929	-4.24	1925:1929	Furthermore, there was an interaction between 3'-SL and age for receptive language (adjusted P = 0.03; EF, -14.93; 95% CI, -25.29 to -4.24).
34020453	11	13	dep	P = 0.03	1885:1892	arg1	%					1909:1909	95% CI	1907:1912	95% CI	1907:1912	Furthermore, there was an interaction between 3'-SL and age for receptive language (adjusted P = 0.03; EF, -14.93; 95% CI, -25.29 to -4.24).
34020453	11	13	dep	P = 0.03	1885:1892	arg1	EF					1895:1896	EF	1895:1896	EF	1895:1896	Furthermore, there was an interaction between 3'-SL and age for receptive language (adjusted P = 0.03; EF, -14.93; 95% CI, -25.29 to -4.24).
34020453	6	14	theme	HMO	1117:1119	arg1	concentrations					1121:1134	age-removed HMO concentrations	1105:1134	age-removed HMO concentrations	1105:1134	Linear mixed effects models were employed by stratifying children based on A-tetra levels (A-tetra+ or A-tetra-) to assess associations between age-removed HMO concentrations and both MSEL composite scores and the 5 subdomain scores.
34020453	7	15	theme	samples	1247:1253	arg1	total					1205:1209	A total	1203:1209	A total of 99 mother-child dyads and 183 HM samples	1203:1253	RESULTS A total of 99 mother-child dyads and 183 HM samples were included (A-tetra+: 57 samples, 33 dyads; A-tetra-: 126 samples, 66 dyads).
34020453	5	16	theme	cognitive	938:946	arg1	development					948:958	early cognitive development	932:958	early cognitive development	932:958	The Mullen Scales of Early Learning (MSEL) were used as the primary outcome measure to assess early cognitive development.
34020453	3	17	theme	cognitive	567:575	arg1	development					577:587	cognitive development	567:587	cognitive development	567:587	OBJECTIVES This study aimed to determine whether associations exist between HMO concentrations and cognitive development, and whether the associations vary between A-tetra+ and A-tetra- groups in children (<25 months old).
34020453	1	18	theme	human	153:157	arg1	composition					186:196	human milk oligosaccharide (HMO) composition	153:196	human milk oligosaccharide (HMO) composition	153:196	BACKGROUND Genetic polymorphisms leading to variations in human milk oligosaccharide (HMO) composition have been reported.
34020453	12	19	theme	language	2016:2023	arg1	functions					2025:2033	language functions	2016:2033	language functions	2016:2033	CONCLUSIONS Our study reports the association of 3'-SL and cognition, particularly language functions, in typically developing children who received HM containing detectable A-tetra during infancy.
34020453	7	20	dep	samples	1283:1289	arg1	A-tetra-					1302:1309	A-tetra-	1302:1309	A-tetra-	1302:1309	RESULTS A total of 99 mother-child dyads and 183 HM samples were included (A-tetra+: 57 samples, 33 dyads; A-tetra-: 126 samples, 66 dyads).
34020453	7	20	dep	samples	1283:1289	arg1	samples					1316:1322	126 samples	1312:1322	126 samples	1312:1322	RESULTS A total of 99 mother-child dyads and 183 HM samples were included (A-tetra+: 57 samples, 33 dyads; A-tetra-: 126 samples, 66 dyads).
34020453	8	21	theme	significant	1339:1349	arg1	association					1351:1361	No significant association	1336:1361	No significant association	1336:1361	No significant association was observed between HMOs and MSEL when all samples were analyzed together.
34020453	1	22	theme	HMO	181:183	arg1	composition					186:196	human milk oligosaccharide (HMO) composition	153:196	human milk oligosaccharide (HMO) composition	153:196	BACKGROUND Genetic polymorphisms leading to variations in human milk oligosaccharide (HMO) composition have been reported.
34020453	10	23	theme	subdomain	1774:1782	arg1	scores					1784:1789	the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores	1634:1789	the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores	1634:1789	This association was driven by the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores.
34020453	10	24	dep	P = 0.048	1724:1732	arg1	2.51-13.79					1753:1762	2.51-13.79	1753:1762	2.51-13.79	1753:1762	This association was driven by the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores.
34020453	10	24	dep	P = 0.048	1724:1732	arg1	EF					1735:1736	EF	1735:1736	EF	1735:1736	This association was driven by the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores.
34020453	10	24	dep	P = 0.048	1724:1732	arg1	%					1747:1747	95% CI	1745:1750	95% CI	1745:1750	This association was driven by the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores.
34020453	4	25	theme	developing	721:730	arg1	children					732:739	typically developing children	711:739	typically developing children (2-25 months old; mean, 10 months old) who were at least partially breastfed at the study visit	711:835	METHODS We enrolled typically developing children (2-25 months old; mean, 10 months old) who were at least partially breastfed at the study visit.
34020453	6	26	theme	mixed	968:972	arg1	models					982:987	Linear mixed effects models	961:987	Linear mixed effects models	961:987	Linear mixed effects models were employed by stratifying children based on A-tetra levels (A-tetra+ or A-tetra-) to assess associations between age-removed HMO concentrations and both MSEL composite scores and the 5 subdomain scores.
34020453	2	27	theme	genetic	387:393	arg1	origins					395:401	genetic origins	387:401	genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM	387:465	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	12	28	dep	CONCLUSIONS	1933:1943	arg1	reports					1955:1961	reports	1955:1961	reports	1955:1961	CONCLUSIONS Our study reports the association of 3'-SL and cognition, particularly language functions, in typically developing children who received HM containing detectable A-tetra during infancy.
34020453	0	29	theme	Human	0:4	arg1	3'-Sialyllactose					11:26	Human milk 3'-Sialyllactose	0:26	Human milk 3'-Sialyllactose	0:26	Human milk 3'-Sialyllactose is positively associated with language development during infancy.
34020453	1	30	theme	Genetic	106:112	arg1	polymorphisms					114:126	BACKGROUND Genetic polymorphisms	95:126	BACKGROUND Genetic polymorphisms leading to variations in human milk oligosaccharide (HMO) composition	95:196	BACKGROUND Genetic polymorphisms leading to variations in human milk oligosaccharide (HMO) composition have been reported.
34020453	7	31	theme	mother-child	1217:1228	arg1	dyads					1230:1234	99 mother-child dyads	1214:1234	99 mother-child dyads	1214:1234	RESULTS A total of 99 mother-child dyads and 183 HM samples were included (A-tetra+: 57 samples, 33 dyads; A-tetra-: 126 samples, 66 dyads).
34020453	3	32	theme	HMO	544:546	arg1	concentrations					548:561	HMO concentrations	544:561	HMO concentrations	544:561	OBJECTIVES This study aimed to determine whether associations exist between HMO concentrations and cognitive development, and whether the associations vary between A-tetra+ and A-tetra- groups in children (<25 months old).
34020453	9	33	theme	composite	1443:1451	arg1	score					1453:1457	composite score	1443:1457	composite score	1443:1457	The composite score and 3'-sialyllactose (3'-SL) levels were positively associated [P = 0.002; effect size (EF), 13.12; 95% CI, 5.36-20.80] in the A-tetra + group.
34020453	2	34	theme	type	368:371	arg1	A					373:373	blood type A	362:373	blood type A	362:373	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	5	35	theme	Early	859:863	arg1	MSEL					875:878	MSEL	875:878	MSEL	875:878	The Mullen Scales of Early Learning (MSEL) were used as the primary outcome measure to assess early cognitive development.
34020453	5	35	theme	Early	859:863	arg1	Learning					865:872	Early Learning	859:872	Early Learning (MSEL)	859:879	The Mullen Scales of Early Learning (MSEL) were used as the primary outcome measure to assess early cognitive development.
34020453	7	36	dep	included	1260:1267	arg1	dyads					1328:1332	66 dyads	1325:1332	66 dyads	1325:1332	RESULTS A total of 99 mother-child dyads and 183 HM samples were included (A-tetra+: 57 samples, 33 dyads; A-tetra-: 126 samples, 66 dyads).
34020453	7	36	dep	included	1260:1267	arg1	samples					1283:1289	A-tetra+: 57 samples	1270:1289	A-tetra+: 57 samples	1270:1289	RESULTS A total of 99 mother-child dyads and 183 HM samples were included (A-tetra+: 57 samples, 33 dyads; A-tetra-: 126 samples, 66 dyads).
34020453	7	36	dep	included	1260:1267	arg1	dyads					1295:1299	33 dyads	1292:1299	33 dyads	1292:1299	RESULTS A total of 99 mother-child dyads and 183 HM samples were included (A-tetra+: 57 samples, 33 dyads; A-tetra-: 126 samples, 66 dyads).
34020453	3	37	dep	children	664:671	arg1	old					685:687	old	685:687	old	685:687	OBJECTIVES This study aimed to determine whether associations exist between HMO concentrations and cognitive development, and whether the associations vary between A-tetra+ and A-tetra- groups in children (<25 months old).
34020453	9	38	theme	score	1453:1457	arg1	levels					1488:1493	The composite score and 3'-sialyllactose (3'-SL) levels	1439:1493	The composite score and 3'-sialyllactose (3'-SL) levels	1439:1493	The composite score and 3'-sialyllactose (3'-SL) levels were positively associated [P = 0.002; effect size (EF), 13.12; 95% CI, 5.36-20.80] in the A-tetra + group.
34020453	9	39	theme	3'-sialyllactose	1463:1478	arg1	levels					1488:1493	The composite score and 3'-sialyllactose (3'-SL) levels	1439:1493	The composite score and 3'-sialyllactose (3'-SL) levels	1439:1493	The composite score and 3'-sialyllactose (3'-SL) levels were positively associated [P = 0.002; effect size (EF), 13.12; 95% CI, 5.36-20.80] in the A-tetra + group.
34020453	5	40	theme	outcome	906:912	arg1	measure					914:920	the primary outcome measure	894:920	the primary outcome measure	894:920	The Mullen Scales of Early Learning (MSEL) were used as the primary outcome measure to assess early cognitive development.
34020453	5	40	theme	outcome	906:912	arg1	Scales					849:854	The Mullen Scales	838:854	The Mullen Scales of Early Learning (MSEL)	838:879	The Mullen Scales of Early Learning (MSEL) were used as the primary outcome measure to assess early cognitive development.
34020453	5	41	used	used	886:889	arg2	measure					914:920	the primary outcome measure	894:920	the primary outcome measure	894:920	The Mullen Scales of Early Learning (MSEL) were used as the primary outcome measure to assess early cognitive development.
34020453	5	41	used	used	886:889	arg2	Scales					849:854	The Mullen Scales	838:854	The Mullen Scales of Early Learning (MSEL)	838:879	The Mullen Scales of Early Learning (MSEL) were used as the primary outcome measure to assess early cognitive development.
34020453	1	42	theme	oligosaccharide	164:178	arg1	composition					186:196	human milk oligosaccharide (HMO) composition	153:196	human milk oligosaccharide (HMO) composition	153:196	BACKGROUND Genetic polymorphisms leading to variations in human milk oligosaccharide (HMO) composition have been reported.
34020453	0	43	theme	milk	6:9	arg1	3'-Sialyllactose					11:26	Human milk 3'-Sialyllactose	0:26	Human milk 3'-Sialyllactose	0:26	Human milk 3'-Sialyllactose is positively associated with language development during infancy.
34020453	11	44	theme	receptive	1856:1864	arg1	language					1866:1873	receptive language	1856:1873	receptive language	1856:1873	Furthermore, there was an interaction between 3'-SL and age for receptive language (adjusted P = 0.03; EF, -14.93; 95% CI, -25.29 to -4.24).
34020453	10	45	dep	P = 0.015	1658:1666	arg1	3.91-15.99					1687:1696	3.91-15.99	1687:1696	3.91-15.99	1687:1696	This association was driven by the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores.
34020453	10	45	dep	P = 0.015	1658:1666	arg1	EF					1669:1670	EF	1669:1670	adjusted P = 0.015; EF	1649:1670	This association was driven by the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores.
34020453	10	45	dep	P = 0.015	1658:1666	arg1	9.95					1673:1676	9.95	1673:1676	9.95	1673:1676	This association was driven by the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores.
34020453	10	45	dep	P = 0.015	1658:1666	arg1	%					1681:1681	95% CI	1679:1684	95% CI	1679:1684	This association was driven by the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores.
34020453	3	46	theme	A-tetra-	645:652	arg1	groups					654:659	A-tetra+ and A-tetra- groups	632:659	A-tetra+ and A-tetra- groups in children (<25 months old)	632:688	OBJECTIVES This study aimed to determine whether associations exist between HMO concentrations and cognitive development, and whether the associations vary between A-tetra+ and A-tetra- groups in children (<25 months old).
34020453	6	47	theme	subdomain	1177:1185	arg1	scores					1187:1192	the 5 subdomain scores	1171:1192	the 5 subdomain scores	1171:1192	Linear mixed effects models were employed by stratifying children based on A-tetra levels (A-tetra+ or A-tetra-) to assess associations between age-removed HMO concentrations and both MSEL composite scores and the 5 subdomain scores.
34020453	4	48	dep	months	747:752	arg1	mean					759:762	mean	759:762	mean	759:762	METHODS We enrolled typically developing children (2-25 months old; mean, 10 months old) who were at least partially breastfed at the study visit.
34020453	4	48	dep	months	747:752	arg1	months					768:773	10 months	765:773	10 months old	765:777	METHODS We enrolled typically developing children (2-25 months old; mean, 10 months old) who were at least partially breastfed at the study visit.
34020453	9	49	theme	effect	1534:1539	arg1	EF					1547:1548	EF	1547:1548	EF	1547:1548	The composite score and 3'-sialyllactose (3'-SL) levels were positively associated [P = 0.002; effect size (EF), 13.12; 95% CI, 5.36-20.80] in the A-tetra + group.
34020453	9	49	theme	effect	1534:1539	arg1	size					1541:1544	effect size	1534:1544	effect size (EF)	1534:1549	The composite score and 3'-sialyllactose (3'-SL) levels were positively associated [P = 0.002; effect size (EF), 13.12; 95% CI, 5.36-20.80] in the A-tetra + group.
34020453	4	50	theme	old	775:777	arg1	months					768:773	10 months	765:773	10 months old	765:777	METHODS We enrolled typically developing children (2-25 months old; mean, 10 months old) who were at least partially breastfed at the study visit.
34020453	2	51	from	milk	338:341	arg1	present					285:291	present	285:291	present	285:291	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	2	52	attach	present	285:291	arg2	A-tetra					241:247	A-tetra	241:247	A-tetra	241:247	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	2	52	attach	present	285:291	arg1	HM					344:345	HM	344:345	HM	344:345	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	2	52	attach	present	285:291	arg2	Alpha-Tetrasaccharide					218:238	Alpha-Tetrasaccharide	218:238	Alpha-Tetrasaccharide (A-tetra)	218:248	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	2	52	attach	present	285:291	arg2	HMO					254:256	an HMO	251:256	an HMO	251:256	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	2	52	attach	present	285:291	arg1	milk					338:341	the human milk	328:341	the human milk (HM) of women with blood type A	328:373	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	4	53	theme	study	825:829	arg1	visit					831:835	the study visit	821:835	the study visit	821:835	METHODS We enrolled typically developing children (2-25 months old; mean, 10 months old) who were at least partially breastfed at the study visit.
34020453	2	54	from	absence	431:437	arg1	HM					464:465	HM	464:465	HM	464:465	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	6	55	theme	A-tetra	1036:1042	arg1	levels					1044:1049	A-tetra levels	1036:1049	A-tetra levels (A-tetra+ or A-tetra-)	1036:1072	Linear mixed effects models were employed by stratifying children based on A-tetra levels (A-tetra+ or A-tetra-) to assess associations between age-removed HMO concentrations and both MSEL composite scores and the 5 subdomain scores.
34020453	6	55	theme	A-tetra	1036:1042	arg1	A-tetra-					1064:1071	A-tetra+ or A-tetra-	1052:1071	A-tetra+ or A-tetra-	1052:1071	Linear mixed effects models were employed by stratifying children based on A-tetra levels (A-tetra+ or A-tetra-) to assess associations between age-removed HMO concentrations and both MSEL composite scores and the 5 subdomain scores.
34020453	12	56	theme	developing	2049:2058	arg1	children					2060:2067	typically developing children	2039:2067	typically developing children who received HM containing detectable A-tetra during infancy	2039:2128	CONCLUSIONS Our study reports the association of 3'-SL and cognition, particularly language functions, in typically developing children who received HM containing detectable A-tetra during infancy.
34020453	3	57	from	groups	654:659	arg1	children					664:671	children	664:671	children (<25 months old)	664:688	OBJECTIVES This study aimed to determine whether associations exist between HMO concentrations and cognitive development, and whether the associations vary between A-tetra+ and A-tetra- groups in children (<25 months old).
34020453	4	58	dep	METHODS	691:697	arg1	enrolled					702:709	enrolled	702:709	enrolled typically developing children (2-25 months old; mean, 10 months old) who were at least partially breastfed at the study visit	702:835	METHODS We enrolled typically developing children (2-25 months old; mean, 10 months old) who were at least partially breastfed at the study visit.
34020453	2	59	dep	present	285:291	arg1	>limit					294:299	>limit	294:299	>limit of detection; A-tetra+	294:322	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	5	60	theme	early	932:936	arg1	development					948:958	early cognitive development	932:958	early cognitive development	932:958	The Mullen Scales of Early Learning (MSEL) were used as the primary outcome measure to assess early cognitive development.
34020453	10	61	theme	adjusted	1649:1656	arg1	P = 0.015					1658:1666	adjusted P = 0.015	1649:1666	adjusted P = 0.015; EF	1649:1670	This association was driven by the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores.
34020453	3	62	dep	OBJECTIVES	468:477	arg1	aimed					490:494	aimed	490:494	aimed to determine whether associations exist between HMO concentrations and cognitive development, and whether the associations vary between A-tetra+ and A-tetra- groups in children (<25 months old)	490:688	OBJECTIVES This study aimed to determine whether associations exist between HMO concentrations and cognitive development, and whether the associations vary between A-tetra+ and A-tetra- groups in children (<25 months old).
34020453	12	63	theme	functions	2025:2033	arg1	association					1967:1977	the association	1963:1977	the association of 3'-SL and cognition, particularly language functions	1963:2033	CONCLUSIONS Our study reports the association of 3'-SL and cognition, particularly language functions, in typically developing children who received HM containing detectable A-tetra during infancy.
34020453	6	64	theme	age-removed	1105:1115	arg1	concentrations					1121:1134	age-removed HMO concentrations	1105:1134	age-removed HMO concentrations	1105:1134	Linear mixed effects models were employed by stratifying children based on A-tetra levels (A-tetra+ or A-tetra-) to assess associations between age-removed HMO concentrations and both MSEL composite scores and the 5 subdomain scores.
34020453	2	65	dep	>limit	294:299	arg1	A-tetra+					315:322	A-tetra+	315:322	>limit of detection; A-tetra+	294:322	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	10	66	theme	receptive	1638:1646	arg1	scores					1784:1789	the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores	1634:1789	the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores	1634:1789	This association was driven by the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores.
34020453	1	67	theme	milk	159:162	arg1	composition					186:196	human milk oligosaccharide (HMO) composition	153:196	human milk oligosaccharide (HMO) composition	153:196	BACKGROUND Genetic polymorphisms leading to variations in human milk oligosaccharide (HMO) composition have been reported.
34020453	12	68	theme	3'-SL	1982:1986	arg1	association					1967:1977	the association	1963:1977	the association of 3'-SL and cognition, particularly language functions	1963:2033	CONCLUSIONS Our study reports the association of 3'-SL and cognition, particularly language functions, in typically developing children who received HM containing detectable A-tetra during infancy.
34020453	7	69	theme	HM	1244:1245	arg1	samples					1247:1253	183 HM samples	1240:1253	183 HM samples	1240:1253	RESULTS A total of 99 mother-child dyads and 183 HM samples were included (A-tetra+: 57 samples, 33 dyads; A-tetra-: 126 samples, 66 dyads).
34020453	2	70	from	present	285:291	arg1	HM					344:345	HM	344:345	HM	344:345	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	2	70	from	present	285:291	arg1	milk					338:341	the human milk	328:341	the human milk (HM) of women with blood type A	328:373	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	12	71	theme	cognition	1992:2000	arg1	association					1967:1977	the association	1963:1977	the association of 3'-SL and cognition, particularly language functions	1963:2033	CONCLUSIONS Our study reports the association of 3'-SL and cognition, particularly language functions, in typically developing children who received HM containing detectable A-tetra during infancy.
34020453	2	72	dep	presence	419:426	arg1	the					415:417	the	415:417	the	415:417	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	2	73	attach	presence	419:426	arg1	HM					464:465	HM	464:465	HM	464:465	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	2	73	attach	presence	419:426	arg2	A-tetra					453:459	A-tetra	453:459	A-tetra	453:459	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	5	74	theme	Learning	865:872	arg1	measure					914:920	the primary outcome measure	894:920	the primary outcome measure	894:920	The Mullen Scales of Early Learning (MSEL) were used as the primary outcome measure to assess early cognitive development.
34020453	5	74	theme	Learning	865:872	arg1	Scales					849:854	The Mullen Scales	838:854	The Mullen Scales of Early Learning (MSEL)	838:879	The Mullen Scales of Early Learning (MSEL) were used as the primary outcome measure to assess early cognitive development.
34020453	2	75	theme	A-tetra	453:459	arg1	A-tetra-					440:447	A-tetra-	440:447	A-tetra-	440:447	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	2	75	theme	A-tetra	453:459	arg1	absence					431:437	absence	431:437	absence	431:437	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	2	75	theme	A-tetra	453:459	arg1	presence					419:426	presence	419:426	presence	419:426	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	10	76	theme	language	1765:1772	arg1	scores					1784:1789	the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores	1634:1789	the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores	1634:1789	This association was driven by the receptive (adjusted P = 0.015; EF, 9.95; 95% CI, 3.91-15.99) and expressive (adjusted P = 0.048; EF, 7.53; 95% CI, 2.51-13.79) language subdomain scores.
34020453	6	77	theme	effects	974:980	arg1	models					982:987	Linear mixed effects models	961:987	Linear mixed effects models	961:987	Linear mixed effects models were employed by stratifying children based on A-tetra levels (A-tetra+ or A-tetra-) to assess associations between age-removed HMO concentrations and both MSEL composite scores and the 5 subdomain scores.
34020453	2	78	from	presence	419:426	arg1	HM					464:465	HM	464:465	HM	464:465	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	9	79	dep	associated	1511:1520	arg1	[P = 0.002					1522:1531	[P = 0.002	1522:1531	[P = 0.002	1522:1531	The composite score and 3'-sialyllactose (3'-SL) levels were positively associated [P = 0.002; effect size (EF), 13.12; 95% CI, 5.36-20.80] in the A-tetra + group.
34020453	5	80	theme	Mullen	842:847	arg1	measure					914:920	the primary outcome measure	894:920	the primary outcome measure	894:920	The Mullen Scales of Early Learning (MSEL) were used as the primary outcome measure to assess early cognitive development.
34020453	5	80	theme	Mullen	842:847	arg1	Scales					849:854	The Mullen Scales	838:854	The Mullen Scales of Early Learning (MSEL)	838:879	The Mullen Scales of Early Learning (MSEL) were used as the primary outcome measure to assess early cognitive development.
34020453	6	81	theme	Linear	961:966	arg1	models					982:987	Linear mixed effects models	961:987	Linear mixed effects models	961:987	Linear mixed effects models were employed by stratifying children based on A-tetra levels (A-tetra+ or A-tetra-) to assess associations between age-removed HMO concentrations and both MSEL composite scores and the 5 subdomain scores.
34020453	12	82	contain	containing	2085:2094	arg1	HM					2082:2083	HM	2082:2083	HM containing detectable A-tetra during infancy	2082:2128	CONCLUSIONS Our study reports the association of 3'-SL and cognition, particularly language functions, in typically developing children who received HM containing detectable A-tetra during infancy.
34020453	12	82	contain	containing	2085:2094	arg2	A-tetra					2107:2113	detectable A-tetra	2096:2113	detectable A-tetra	2096:2113	CONCLUSIONS Our study reports the association of 3'-SL and cognition, particularly language functions, in typically developing children who received HM containing detectable A-tetra during infancy.
34020453	1	83	from	variations	139:148	arg1	composition					186:196	human milk oligosaccharide (HMO) composition	153:196	human milk oligosaccharide (HMO) composition	153:196	BACKGROUND Genetic polymorphisms leading to variations in human milk oligosaccharide (HMO) composition have been reported.
34020453	6	84	theme	MSEL	1145:1148	arg1	scores					1160:1165	MSEL composite scores	1145:1165	MSEL composite scores	1145:1165	Linear mixed effects models were employed by stratifying children based on A-tetra levels (A-tetra+ or A-tetra-) to assess associations between age-removed HMO concentrations and both MSEL composite scores and the 5 subdomain scores.
34020453	2	85	located	present	285:291	arg2	A-tetra					241:247	A-tetra	241:247	A-tetra	241:247	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	2	85	located	present	285:291	arg1	HM					344:345	HM	344:345	HM	344:345	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	2	85	located	present	285:291	arg2	Alpha-Tetrasaccharide					218:238	Alpha-Tetrasaccharide	218:238	Alpha-Tetrasaccharide (A-tetra)	218:248	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	2	85	located	present	285:291	arg2	HMO					254:256	an HMO	251:256	an HMO	251:256	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	2	85	located	present	285:291	arg1	milk					338:341	the human milk	328:341	the human milk (HM) of women with blood type A	328:373	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	2	86	theme	blood	362:366	arg1	A					373:373	blood type A	362:373	blood type A	362:373	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	5	87	theme	primary	898:904	arg1	measure					914:920	the primary outcome measure	894:920	the primary outcome measure	894:920	The Mullen Scales of Early Learning (MSEL) were used as the primary outcome measure to assess early cognitive development.
34020453	5	87	theme	primary	898:904	arg1	Scales					849:854	The Mullen Scales	838:854	The Mullen Scales of Early Learning (MSEL)	838:879	The Mullen Scales of Early Learning (MSEL) were used as the primary outcome measure to assess early cognitive development.
34020453	7	88	theme	dyads	1230:1234	arg1	total					1205:1209	A total	1203:1209	A total of 99 mother-child dyads and 183 HM samples	1203:1253	RESULTS A total of 99 mother-child dyads and 183 HM samples were included (A-tetra+: 57 samples, 33 dyads; A-tetra-: 126 samples, 66 dyads).
34020453	11	89	dep	-4.24	1925:1929	arg1	to					1922:1923	to	1922:1923	to	1922:1923	Furthermore, there was an interaction between 3'-SL and age for receptive language (adjusted P = 0.03; EF, -14.93; 95% CI, -25.29 to -4.24).
34020453	4	90	theme	old	754:756	arg1	months					747:752	2-25 months old	742:756	2-25 months old	742:756	METHODS We enrolled typically developing children (2-25 months old; mean, 10 months old) who were at least partially breastfed at the study visit.
34020453	2	91	theme	women	351:355	arg1	HM					344:345	HM	344:345	HM	344:345	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	2	91	theme	women	351:355	arg1	milk					338:341	the human milk	328:341	the human milk (HM) of women with blood type A	328:373	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	0	92	theme	language	58:65	arg1	development					67:77	language development	58:77	language development	58:77	Human milk 3'-Sialyllactose is positively associated with language development during infancy.
34020453	7	93	dep	RESULTS	1195:1201	arg1	included					1260:1267	included	1260:1267	were included (A-tetra+: 57 samples, 33 dyads; A-tetra-: 126 samples, 66 dyads)	1255:1333	RESULTS A total of 99 mother-child dyads and 183 HM samples were included (A-tetra+: 57 samples, 33 dyads; A-tetra-: 126 samples, 66 dyads).
34020453	12	94	theme	detectable	2096:2105	arg1	A-tetra					2107:2113	detectable A-tetra	2096:2113	detectable A-tetra	2096:2113	CONCLUSIONS Our study reports the association of 3'-SL and cognition, particularly language functions, in typically developing children who received HM containing detectable A-tetra during infancy.
34020453	2	95	theme	detection	304:312	arg1	>limit					294:299	>limit	294:299	>limit of detection; A-tetra+	294:322	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	2	96	theme	human	332:336	arg1	HM					344:345	HM	344:345	HM	344:345	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34020453	2	96	theme	human	332:336	arg1	milk					338:341	the human milk	328:341	the human milk (HM) of women with blood type A	328:373	Alpha-Tetrasaccharide (A-tetra), an HMO, has been shown to only be present (>limit of detection; A-tetra+) in the human milk (HM) of women with blood type A, suggesting genetic origins determining the presence or absence (A-tetra-) of A-tetra in HM.
34699897	6	0	theme	study	1233:1237	arg1	results					1217:1223	The results	1213:1223	The results of this study	1213:1237	The results of this study highlight the great potential of the prepared biomimetic HLC/oxi-HA bio-composites as a new generation of multifunctional HA-based wound-healing materials.
34699897	4	1	theme	membranes	732:740	arg1	preparation					742:752	HA/HLC bio-composite membranes preparation	711:752	HA/HLC bio-composite membranes preparation	711:752	Herein, we developed a novel method for HA/HLC bio-composite membranes preparation, comprising one-step surface modification-assembly process by which the HLC self-assembles are simultaneously loaded on the oxidized-modified HA (oxi-HA) from the surface/interface micro-scale.
34699897	3	2	theme	worldwide	660:668	arg1	attention					650:658	extensive attention	640:658	extensive attention worldwide	640:668	Human like collagen (HLC), which is hydrophilic, biomimetic, and bio-safe, with human tissue-derived collagen biofunction, has attracted extensive attention worldwide.
34699897	0	3	theme	facile	84:89	arg1	approach					123:130	A facile "surface modification-assembly" approach	82:130	Development of novel hyaluronic acid/human-like collagen bio-composite membranes: A facile "surface modification-assembly" approach.	0:131	Development of novel hyaluronic acid/human-like collagen bio-composite membranes: A facile "surface modification-assembly" approach.
34699897	0	4	theme	modification-assembly	100:120	arg1	approach					123:130	A facile "surface modification-assembly" approach	82:130	Development of novel hyaluronic acid/human-like collagen bio-composite membranes: A facile "surface modification-assembly" approach.	0:131	Development of novel hyaluronic acid/human-like collagen bio-composite membranes: A facile "surface modification-assembly" approach.
34699897	6	5	theme	biomimetic	1285:1294	arg1	bio-composites					1307:1320	the prepared biomimetic HLC/oxi-HA bio-composites	1272:1320	the prepared biomimetic HLC/oxi-HA bio-composites	1272:1320	The results of this study highlight the great potential of the prepared biomimetic HLC/oxi-HA bio-composites as a new generation of multifunctional HA-based wound-healing materials.
34699897	5	6	theme	in	993:994	arg1	biostudies					1010:1019	in vitro/in vivo biostudies	993:1019	in vitro/in vivo biostudies	993:1019	Comprehensive material characterizations and in vitro/in vivo biostudies proved that the HLC/oxi-HA composite membranes exhibited significantly enhanced biological activity, hemostatic performances, and wound healing properties compared to that of the pristine HA.
34699897	5	7	dep	in	993:994	arg1	vivo					1005:1008	vivo	1005:1008	vivo	1005:1008	Comprehensive material characterizations and in vitro/in vivo biostudies proved that the HLC/oxi-HA composite membranes exhibited significantly enhanced biological activity, hemostatic performances, and wound healing properties compared to that of the pristine HA.
34699897	1	8	theme	ideal	274:278	arg1	block					289:293	ideal building block	274:293	ideal building block for the engineering of tissue engineering scaffolds	274:345	The merits of hyaluronic acid (HA) as a representative biological carbohydrate polymers especially in bioactivity and tailorability makes it ideal building block for the engineering of tissue engineering scaffolds.
34699897	0	9	theme	surface	92:98	arg1	approach					123:130	A facile "surface modification-assembly" approach	82:130	Development of novel hyaluronic acid/human-like collagen bio-composite membranes: A facile "surface modification-assembly" approach.	0:131	Development of novel hyaluronic acid/human-like collagen bio-composite membranes: A facile "surface modification-assembly" approach.
34699897	4	10	from	micro-scale	935:945	arg1	oxi-HA					900:905	oxi-HA	900:905	oxi-HA	900:905	Herein, we developed a novel method for HA/HLC bio-composite membranes preparation, comprising one-step surface modification-assembly process by which the HLC self-assembles are simultaneously loaded on the oxidized-modified HA (oxi-HA) from the surface/interface micro-scale.
34699897	4	10	from	micro-scale	935:945	arg1	HA					896:897	the oxidized-modified HA	874:897	the oxidized-modified HA (oxi-HA) from the surface/interface micro-scale	874:945	Herein, we developed a novel method for HA/HLC bio-composite membranes preparation, comprising one-step surface modification-assembly process by which the HLC self-assembles are simultaneously loaded on the oxidized-modified HA (oxi-HA) from the surface/interface micro-scale.
34699897	5	11	theme	enhanced	1092:1099	arg1	activity					1112:1119	significantly enhanced biological activity	1078:1119	significantly enhanced biological activity	1078:1119	Comprehensive material characterizations and in vitro/in vivo biostudies proved that the HLC/oxi-HA composite membranes exhibited significantly enhanced biological activity, hemostatic performances, and wound healing properties compared to that of the pristine HA.
34699897	4	12	theme	HLC	826:828	arg1	self-assembles					830:843	the HLC self-assembles	822:843	the HLC self-assembles	822:843	Herein, we developed a novel method for HA/HLC bio-composite membranes preparation, comprising one-step surface modification-assembly process by which the HLC self-assembles are simultaneously loaded on the oxidized-modified HA (oxi-HA) from the surface/interface micro-scale.
34699897	1	13	theme	building	280:287	arg1	block					289:293	ideal building block	274:293	ideal building block for the engineering of tissue engineering scaffolds	274:345	The merits of hyaluronic acid (HA) as a representative biological carbohydrate polymers especially in bioactivity and tailorability makes it ideal building block for the engineering of tissue engineering scaffolds.
34699897	6	14	theme	great	1253:1257	arg1	generation					1331:1340	a new generation	1325:1340	a new generation of multifunctional HA-based wound-healing materials	1325:1392	The results of this study highlight the great potential of the prepared biomimetic HLC/oxi-HA bio-composites as a new generation of multifunctional HA-based wound-healing materials.
34699897	6	14	theme	great	1253:1257	arg1	potential					1259:1267	the great potential	1249:1267	the great potential of the prepared biomimetic HLC/oxi-HA bio-composites	1249:1320	The results of this study highlight the great potential of the prepared biomimetic HLC/oxi-HA bio-composites as a new generation of multifunctional HA-based wound-healing materials.
34699897	5	15	theme	biological	1101:1110	arg1	activity					1112:1119	significantly enhanced biological activity	1078:1119	significantly enhanced biological activity	1078:1119	Comprehensive material characterizations and in vitro/in vivo biostudies proved that the HLC/oxi-HA composite membranes exhibited significantly enhanced biological activity, hemostatic performances, and wound healing properties compared to that of the pristine HA.
34699897	5	16	theme	material	962:969	arg1	characterizations					971:987	Comprehensive material characterizations	948:987	Comprehensive material characterizations	948:987	Comprehensive material characterizations and in vitro/in vivo biostudies proved that the HLC/oxi-HA composite membranes exhibited significantly enhanced biological activity, hemostatic performances, and wound healing properties compared to that of the pristine HA.
34699897	0	17	theme	"	121:121	arg1	approach					123:130	A facile "surface modification-assembly" approach	82:130	Development of novel hyaluronic acid/human-like collagen bio-composite membranes: A facile "surface modification-assembly" approach.	0:131	Development of novel hyaluronic acid/human-like collagen bio-composite membranes: A facile "surface modification-assembly" approach.
34699897	5	18	theme	HLC/oxi-HA	1037:1046	arg1	membranes					1058:1066	the HLC/oxi-HA composite membranes	1033:1066	the HLC/oxi-HA composite membranes	1033:1066	Comprehensive material characterizations and in vitro/in vivo biostudies proved that the HLC/oxi-HA composite membranes exhibited significantly enhanced biological activity, hemostatic performances, and wound healing properties compared to that of the pristine HA.
34699897	4	19	theme	novel	694:698	arg1	method					700:705	a novel method	692:705	a novel method for HA/HLC bio-composite membranes preparation	692:752	Herein, we developed a novel method for HA/HLC bio-composite membranes preparation, comprising one-step surface modification-assembly process by which the HLC self-assembles are simultaneously loaded on the oxidized-modified HA (oxi-HA) from the surface/interface micro-scale.
34699897	6	20	theme	prepared	1276:1283	arg1	bio-composites					1307:1320	the prepared biomimetic HLC/oxi-HA bio-composites	1272:1320	the prepared biomimetic HLC/oxi-HA bio-composites	1272:1320	The results of this study highlight the great potential of the prepared biomimetic HLC/oxi-HA bio-composites as a new generation of multifunctional HA-based wound-healing materials.
34699897	3	21	theme	collagen	604:611	arg1	biofunction					613:623	human tissue-derived collagen biofunction	583:623	human tissue-derived collagen biofunction	583:623	Human like collagen (HLC), which is hydrophilic, biomimetic, and bio-safe, with human tissue-derived collagen biofunction, has attracted extensive attention worldwide.
34699897	4	22	theme	HA/HLC	711:716	arg1	membranes					732:740	HA/HLC bio-composite membranes	711:740	HA/HLC bio-composite membranes preparation	711:752	Herein, we developed a novel method for HA/HLC bio-composite membranes preparation, comprising one-step surface modification-assembly process by which the HLC self-assembles are simultaneously loaded on the oxidized-modified HA (oxi-HA) from the surface/interface micro-scale.
34699897	4	23	theme	surface	775:781	arg1	process					805:811	one-step surface modification-assembly process	766:811	one-step surface modification-assembly process by which the HLC self-assembles are simultaneously loaded on the oxidized-modified HA (oxi-HA) from the surface/interface micro-scale	766:945	Herein, we developed a novel method for HA/HLC bio-composite membranes preparation, comprising one-step surface modification-assembly process by which the HLC self-assembles are simultaneously loaded on the oxidized-modified HA (oxi-HA) from the surface/interface micro-scale.
34699897	5	24	theme	composite	1048:1056	arg1	membranes					1058:1066	the HLC/oxi-HA composite membranes	1033:1066	the HLC/oxi-HA composite membranes	1033:1066	Comprehensive material characterizations and in vitro/in vivo biostudies proved that the HLC/oxi-HA composite membranes exhibited significantly enhanced biological activity, hemostatic performances, and wound healing properties compared to that of the pristine HA.
34699897	1	25	theme	representative	173:186	arg1	polymers					212:219	a representative biological carbohydrate polymers	171:219	a representative biological carbohydrate polymers especially in bioactivity and tailorability	171:263	The merits of hyaluronic acid (HA) as a representative biological carbohydrate polymers especially in bioactivity and tailorability makes it ideal building block for the engineering of tissue engineering scaffolds.
34699897	0	26	theme	hyaluronic	21:30	arg1	membranes					71:79	novel hyaluronic acid/human-like collagen bio-composite membranes	15:79	novel hyaluronic acid/human-like collagen bio-composite membranes	15:79	Development of novel hyaluronic acid/human-like collagen bio-composite membranes: A facile "surface modification-assembly" approach.
34699897	6	27	theme	new	1327:1329	arg1	generation					1331:1340	a new generation	1325:1340	a new generation of multifunctional HA-based wound-healing materials	1325:1392	The results of this study highlight the great potential of the prepared biomimetic HLC/oxi-HA bio-composites as a new generation of multifunctional HA-based wound-healing materials.
34699897	6	27	theme	new	1327:1329	arg1	potential					1259:1267	the great potential	1249:1267	the great potential of the prepared biomimetic HLC/oxi-HA bio-composites	1249:1320	The results of this study highlight the great potential of the prepared biomimetic HLC/oxi-HA bio-composites as a new generation of multifunctional HA-based wound-healing materials.
34699897	2	28	dep	integrate	372:380	arg1	possessing					432:441	possessing	432:441	possessing versatility	432:453	HA-based bio-composites integrate the characteristics of multi-component materials, possessing versatility and further improving the therapeutic efficacy.
34699897	2	28	dep	integrate	372:380	arg1	improving					467:475	improving	467:475	further improving the therapeutic efficacy	459:500	HA-based bio-composites integrate the characteristics of multi-component materials, possessing versatility and further improving the therapeutic efficacy.
34699897	1	29	theme	biological	188:197	arg1	polymers					212:219	a representative biological carbohydrate polymers	171:219	a representative biological carbohydrate polymers especially in bioactivity and tailorability	171:263	The merits of hyaluronic acid (HA) as a representative biological carbohydrate polymers especially in bioactivity and tailorability makes it ideal building block for the engineering of tissue engineering scaffolds.
34699897	0	30	theme	novel	15:19	arg1	membranes					71:79	novel hyaluronic acid/human-like collagen bio-composite membranes	15:79	novel hyaluronic acid/human-like collagen bio-composite membranes	15:79	Development of novel hyaluronic acid/human-like collagen bio-composite membranes: A facile "surface modification-assembly" approach.
34699897	2	31	theme	multi-component	405:419	arg1	materials					421:429	multi-component materials	405:429	multi-component materials	405:429	HA-based bio-composites integrate the characteristics of multi-component materials, possessing versatility and further improving the therapeutic efficacy.
34699897	3	32	theme	tissue-derived	589:602	arg1	biofunction					613:623	human tissue-derived collagen biofunction	583:623	human tissue-derived collagen biofunction	583:623	Human like collagen (HLC), which is hydrophilic, biomimetic, and bio-safe, with human tissue-derived collagen biofunction, has attracted extensive attention worldwide.
34699897	1	33	theme	carbohydrate	199:210	arg1	polymers					212:219	a representative biological carbohydrate polymers	171:219	a representative biological carbohydrate polymers especially in bioactivity and tailorability	171:263	The merits of hyaluronic acid (HA) as a representative biological carbohydrate polymers especially in bioactivity and tailorability makes it ideal building block for the engineering of tissue engineering scaffolds.
34699897	0	34	theme	collagen	48:55	arg1	membranes					71:79	novel hyaluronic acid/human-like collagen bio-composite membranes	15:79	novel hyaluronic acid/human-like collagen bio-composite membranes	15:79	Development of novel hyaluronic acid/human-like collagen bio-composite membranes: A facile "surface modification-assembly" approach.
34699897	6	35	theme	wound-healing	1370:1382	arg1	materials					1384:1392	multifunctional HA-based wound-healing materials	1345:1392	multifunctional HA-based wound-healing materials	1345:1392	The results of this study highlight the great potential of the prepared biomimetic HLC/oxi-HA bio-composites as a new generation of multifunctional HA-based wound-healing materials.
34699897	5	36	theme	pristine	1200:1207	arg1	HA					1209:1210	the pristine HA	1196:1210	the pristine HA	1196:1210	Comprehensive material characterizations and in vitro/in vivo biostudies proved that the HLC/oxi-HA composite membranes exhibited significantly enhanced biological activity, hemostatic performances, and wound healing properties compared to that of the pristine HA.
34699897	6	37	theme	materials	1384:1392	arg1	generation					1331:1340	a new generation	1325:1340	a new generation of multifunctional HA-based wound-healing materials	1325:1392	The results of this study highlight the great potential of the prepared biomimetic HLC/oxi-HA bio-composites as a new generation of multifunctional HA-based wound-healing materials.
34699897	6	37	theme	materials	1384:1392	arg1	potential					1259:1267	the great potential	1249:1267	the great potential of the prepared biomimetic HLC/oxi-HA bio-composites	1249:1320	The results of this study highlight the great potential of the prepared biomimetic HLC/oxi-HA bio-composites as a new generation of multifunctional HA-based wound-healing materials.
34699897	2	38	theme	materials	421:429	arg1	characteristics					386:400	the characteristics	382:400	the characteristics of multi-component materials	382:429	HA-based bio-composites integrate the characteristics of multi-component materials, possessing versatility and further improving the therapeutic efficacy.
34699897	0	39	theme	acid/human-like	32:46	arg1	membranes					71:79	novel hyaluronic acid/human-like collagen bio-composite membranes	15:79	novel hyaluronic acid/human-like collagen bio-composite membranes	15:79	Development of novel hyaluronic acid/human-like collagen bio-composite membranes: A facile "surface modification-assembly" approach.
34699897	6	40	theme	bio-composites	1307:1320	arg1	generation					1331:1340	a new generation	1325:1340	a new generation of multifunctional HA-based wound-healing materials	1325:1392	The results of this study highlight the great potential of the prepared biomimetic HLC/oxi-HA bio-composites as a new generation of multifunctional HA-based wound-healing materials.
34699897	6	40	theme	bio-composites	1307:1320	arg1	potential					1259:1267	the great potential	1249:1267	the great potential of the prepared biomimetic HLC/oxi-HA bio-composites	1249:1320	The results of this study highlight the great potential of the prepared biomimetic HLC/oxi-HA bio-composites as a new generation of multifunctional HA-based wound-healing materials.
34699897	3	41	theme	extensive	640:648	arg1	attention					650:658	extensive attention	640:658	extensive attention worldwide	640:668	Human like collagen (HLC), which is hydrophilic, biomimetic, and bio-safe, with human tissue-derived collagen biofunction, has attracted extensive attention worldwide.
34699897	1	42	from	polymers	212:219	arg1	tailorability					251:263	tailorability	251:263	tailorability	251:263	The merits of hyaluronic acid (HA) as a representative biological carbohydrate polymers especially in bioactivity and tailorability makes it ideal building block for the engineering of tissue engineering scaffolds.
34699897	1	42	from	polymers	212:219	arg1	bioactivity					235:245	bioactivity	235:245	bioactivity	235:245	The merits of hyaluronic acid (HA) as a representative biological carbohydrate polymers especially in bioactivity and tailorability makes it ideal building block for the engineering of tissue engineering scaffolds.
34699897	0	43	theme	membranes	71:79	arg1	Development					0:10	Development	0:10	Development of novel hyaluronic acid/human-like collagen bio-composite membranes: A facile "surface modification-assembly" approach.	0:131	Development of novel hyaluronic acid/human-like collagen bio-composite membranes: A facile "surface modification-assembly" approach.
34699897	1	44	theme	tissue	318:323	arg1	scaffolds					337:345	tissue engineering scaffolds	318:345	tissue engineering scaffolds	318:345	The merits of hyaluronic acid (HA) as a representative biological carbohydrate polymers especially in bioactivity and tailorability makes it ideal building block for the engineering of tissue engineering scaffolds.
34699897	6	45	theme	HA-based	1361:1368	arg1	materials					1384:1392	multifunctional HA-based wound-healing materials	1345:1392	multifunctional HA-based wound-healing materials	1345:1392	The results of this study highlight the great potential of the prepared biomimetic HLC/oxi-HA bio-composites as a new generation of multifunctional HA-based wound-healing materials.
34699897	4	46	theme	surface/interface	917:933	arg1	micro-scale					935:945	the surface/interface micro-scale	913:945	the surface/interface micro-scale	913:945	Herein, we developed a novel method for HA/HLC bio-composite membranes preparation, comprising one-step surface modification-assembly process by which the HLC self-assembles are simultaneously loaded on the oxidized-modified HA (oxi-HA) from the surface/interface micro-scale.
34699897	4	47	theme	one-step	766:773	arg1	process					805:811	one-step surface modification-assembly process	766:811	one-step surface modification-assembly process by which the HLC self-assembles are simultaneously loaded on the oxidized-modified HA (oxi-HA) from the surface/interface micro-scale	766:945	Herein, we developed a novel method for HA/HLC bio-composite membranes preparation, comprising one-step surface modification-assembly process by which the HLC self-assembles are simultaneously loaded on the oxidized-modified HA (oxi-HA) from the surface/interface micro-scale.
34699897	0	48	theme	bio-composite	57:69	arg1	membranes					71:79	novel hyaluronic acid/human-like collagen bio-composite membranes	15:79	novel hyaluronic acid/human-like collagen bio-composite membranes	15:79	Development of novel hyaluronic acid/human-like collagen bio-composite membranes: A facile "surface modification-assembly" approach.
34699897	1	49	theme	engineering	325:335	arg1	scaffolds					337:345	tissue engineering scaffolds	318:345	tissue engineering scaffolds	318:345	The merits of hyaluronic acid (HA) as a representative biological carbohydrate polymers especially in bioactivity and tailorability makes it ideal building block for the engineering of tissue engineering scaffolds.
34699897	6	50	theme	multifunctional	1345:1359	arg1	materials					1384:1392	multifunctional HA-based wound-healing materials	1345:1392	multifunctional HA-based wound-healing materials	1345:1392	The results of this study highlight the great potential of the prepared biomimetic HLC/oxi-HA bio-composites as a new generation of multifunctional HA-based wound-healing materials.
34699897	5	51	dep	activity	1112:1119	arg1	properties					1165:1174	properties	1165:1174	properties	1165:1174	Comprehensive material characterizations and in vitro/in vivo biostudies proved that the HLC/oxi-HA composite membranes exhibited significantly enhanced biological activity, hemostatic performances, and wound healing properties compared to that of the pristine HA.
34699897	1	52	theme	scaffolds	337:345	arg1	engineering					303:313	the engineering	299:313	the engineering of tissue engineering scaffolds	299:345	The merits of hyaluronic acid (HA) as a representative biological carbohydrate polymers especially in bioactivity and tailorability makes it ideal building block for the engineering of tissue engineering scaffolds.
34699897	6	53	theme	HLC/oxi-HA	1296:1305	arg1	bio-composites					1307:1320	the prepared biomimetic HLC/oxi-HA bio-composites	1272:1320	the prepared biomimetic HLC/oxi-HA bio-composites	1272:1320	The results of this study highlight the great potential of the prepared biomimetic HLC/oxi-HA bio-composites as a new generation of multifunctional HA-based wound-healing materials.
34699897	5	54	theme	wound	1151:1155	arg1	healing					1157:1163	wound healing	1151:1163	wound healing	1151:1163	Comprehensive material characterizations and in vitro/in vivo biostudies proved that the HLC/oxi-HA composite membranes exhibited significantly enhanced biological activity, hemostatic performances, and wound healing properties compared to that of the pristine HA.
34699897	2	55	theme	HA-based	348:355	arg1	bio-composites					357:370	HA-based bio-composites	348:370	HA-based bio-composites	348:370	HA-based bio-composites integrate the characteristics of multi-component materials, possessing versatility and further improving the therapeutic efficacy.
34699897	3	56	theme	human	583:587	arg1	biofunction					613:623	human tissue-derived collagen biofunction	583:623	human tissue-derived collagen biofunction	583:623	Human like collagen (HLC), which is hydrophilic, biomimetic, and bio-safe, with human tissue-derived collagen biofunction, has attracted extensive attention worldwide.
34699897	3	57	with	Human	503:507	arg1	biofunction					613:623	human tissue-derived collagen biofunction	583:623	human tissue-derived collagen biofunction	583:623	Human like collagen (HLC), which is hydrophilic, biomimetic, and bio-safe, with human tissue-derived collagen biofunction, has attracted extensive attention worldwide.
34699897	5	58	theme	hemostatic	1122:1131	arg1	performances					1133:1144	hemostatic performances	1122:1144	hemostatic performances	1122:1144	Comprehensive material characterizations and in vitro/in vivo biostudies proved that the HLC/oxi-HA composite membranes exhibited significantly enhanced biological activity, hemostatic performances, and wound healing properties compared to that of the pristine HA.
34699897	4	59	theme	bio-composite	718:730	arg1	membranes					732:740	HA/HLC bio-composite membranes	711:740	HA/HLC bio-composite membranes preparation	711:752	Herein, we developed a novel method for HA/HLC bio-composite membranes preparation, comprising one-step surface modification-assembly process by which the HLC self-assembles are simultaneously loaded on the oxidized-modified HA (oxi-HA) from the surface/interface micro-scale.
34699897	4	60	theme	modification-assembly	783:803	arg1	process					805:811	one-step surface modification-assembly process	766:811	one-step surface modification-assembly process by which the HLC self-assembles are simultaneously loaded on the oxidized-modified HA (oxi-HA) from the surface/interface micro-scale	766:945	Herein, we developed a novel method for HA/HLC bio-composite membranes preparation, comprising one-step surface modification-assembly process by which the HLC self-assembles are simultaneously loaded on the oxidized-modified HA (oxi-HA) from the surface/interface micro-scale.
34699897	4	61	theme	oxidized-modified	878:894	arg1	oxi-HA					900:905	oxi-HA	900:905	oxi-HA	900:905	Herein, we developed a novel method for HA/HLC bio-composite membranes preparation, comprising one-step surface modification-assembly process by which the HLC self-assembles are simultaneously loaded on the oxidized-modified HA (oxi-HA) from the surface/interface micro-scale.
34699897	4	61	theme	oxidized-modified	878:894	arg1	HA					896:897	the oxidized-modified HA	874:897	the oxidized-modified HA (oxi-HA) from the surface/interface micro-scale	874:945	Herein, we developed a novel method for HA/HLC bio-composite membranes preparation, comprising one-step surface modification-assembly process by which the HLC self-assembles are simultaneously loaded on the oxidized-modified HA (oxi-HA) from the surface/interface micro-scale.
34699897	2	62	theme	therapeutic	481:491	arg1	efficacy					493:500	the therapeutic efficacy	477:500	the therapeutic efficacy	477:500	HA-based bio-composites integrate the characteristics of multi-component materials, possessing versatility and further improving the therapeutic efficacy.
34699897	1	63	theme	hyaluronic	147:156	arg1	acid					158:161	hyaluronic acid	147:161	hyaluronic acid (HA)	147:166	The merits of hyaluronic acid (HA) as a representative biological carbohydrate polymers especially in bioactivity and tailorability makes it ideal building block for the engineering of tissue engineering scaffolds.
34699897	1	63	theme	hyaluronic	147:156	arg1	HA					164:165	HA	164:165	HA	164:165	The merits of hyaluronic acid (HA) as a representative biological carbohydrate polymers especially in bioactivity and tailorability makes it ideal building block for the engineering of tissue engineering scaffolds.
34699897	5	64	theme	Comprehensive	948:960	arg1	characterizations					971:987	Comprehensive material characterizations	948:987	Comprehensive material characterizations	948:987	Comprehensive material characterizations and in vitro/in vivo biostudies proved that the HLC/oxi-HA composite membranes exhibited significantly enhanced biological activity, hemostatic performances, and wound healing properties compared to that of the pristine HA.
34699897	3	65	link	tissue-derived	589:602	arg1	biofunction					613:623	human tissue-derived collagen biofunction	583:623	human tissue-derived collagen biofunction	583:623	Human like collagen (HLC), which is hydrophilic, biomimetic, and bio-safe, with human tissue-derived collagen biofunction, has attracted extensive attention worldwide.
34699897	0	66	dep	Development	0:10	arg1	approach					123:130	A facile "surface modification-assembly" approach	82:130	Development of novel hyaluronic acid/human-like collagen bio-composite membranes: A facile "surface modification-assembly" approach.	0:131	Development of novel hyaluronic acid/human-like collagen bio-composite membranes: A facile "surface modification-assembly" approach.
34699897	1	67	theme	acid	158:161	arg1	merits					137:142	The merits	133:142	The merits of hyaluronic acid (HA) as a representative biological carbohydrate polymers especially in bioactivity and tailorability	133:263	The merits of hyaluronic acid (HA) as a representative biological carbohydrate polymers especially in bioactivity and tailorability makes it ideal building block for the engineering of tissue engineering scaffolds.
32650193	0	0	theme	polysaccharide	94:107	arg1	phase					120:124	a polysaccharide stationary phase	92:124	a polysaccharide stationary phase	92:124	Separation of achiral anti-diabetic drugs using sub/supercritical fluid chromatography with a polysaccharide stationary phase: Thermodynamic considerations and molecular docking study.
32650193	8	1	theme	acceptable	1612:1621	arg1	recovery					1633:1640	recovery	1633:1640	recovery	1633:1640	The method was used to analyze metformin and SGLT-2 inhibitors in their binary fixed-dose formulations with acceptable accuracy (recovery) and precision.
32650193	8	1	theme	acceptable	1612:1621	arg1	accuracy					1623:1630	acceptable accuracy	1612:1630	acceptable accuracy (recovery)	1612:1641	The method was used to analyze metformin and SGLT-2 inhibitors in their binary fixed-dose formulations with acceptable accuracy (recovery) and precision.
32650193	6	2	theme	metformin-empagliflozin	1314:1336	arg1	pairs					1338:1342	metformin-canagliflozin, metformin-dapagliflozin and metformin-empagliflozin pairs	1261:1342	metformin-canagliflozin, metformin-dapagliflozin and metformin-empagliflozin pairs	1261:1342	The chromatographic resolution between metformin-canagliflozin, metformin-dapagliflozin and metformin-empagliflozin pairs was 6.87, 3.44 and 6.47, respectively.
32650193	3	3	theme	based	750:754	arg1	column					769:774	amylose based Chiralpak IG column	742:774	amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase	742:883	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	9	4	theme	molecular	1695:1703	arg1	docking					1705:1711	molecular docking	1695:1711	molecular docking	1695:1711	To support the experimental results, molecular docking was performed on Schrodinger software to provide a deeper insight into the interactions between the analytes and the chiral stationary phase and to understand their elution orders under optimized conditions.
32650193	0	5	dep	considerations	141:154	arg1	Separation					0:9	Separation	0:9	Separation of achiral anti-diabetic drugs using sub/supercritical fluid chromatography with a polysaccharide stationary phase	0:124	Separation of achiral anti-diabetic drugs using sub/supercritical fluid chromatography with a polysaccharide stationary phase: Thermodynamic considerations and molecular docking study.
32650193	3	6	theme	mobile	872:877	arg1	CO2					782:784	CO2	782:784	CO2	782:784	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	3	6	theme	mobile	872:877	arg1	phase					879:883	the mobile phase	868:883	the mobile phase	868:883	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	3	6	theme	mobile	872:877	arg1	diethylamine					796:807	0.1 % diethylamine	790:807	0.1 % diethylamine	790:807	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	4	7	theme	phase	982:986	arg1	composition					988:998	the mobile phase composition	971:998	the mobile phase composition	971:998	The separation efficiency was mainly dependent on the nature of stationary phase and the mobile phase composition.
32650193	3	8	theme	Chiralpak	756:764	arg1	column					769:774	amylose based Chiralpak IG column	742:774	amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase	742:883	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	6	9	theme	metformin-dapagliflozin	1286:1308	arg1	pairs					1338:1342	metformin-canagliflozin, metformin-dapagliflozin and metformin-empagliflozin pairs	1261:1342	metformin-canagliflozin, metformin-dapagliflozin and metformin-empagliflozin pairs	1261:1342	The chromatographic resolution between metformin-canagliflozin, metformin-dapagliflozin and metformin-empagliflozin pairs was 6.87, 3.44 and 6.47, respectively.
32650193	9	10	theme	chiral	1830:1835	arg1	phase					1848:1852	the chiral stationary phase	1826:1852	the chiral stationary phase	1826:1852	To support the experimental results, molecular docking was performed on Schrodinger software to provide a deeper insight into the interactions between the analytes and the chiral stationary phase and to understand their elution orders under optimized conditions.
32650193	8	11	theme	binary	1576:1581	arg1	formulations					1594:1605	their binary fixed-dose formulations	1570:1605	their binary fixed-dose formulations with acceptable accuracy (recovery) and precision	1570:1655	The method was used to analyze metformin and SGLT-2 inhibitors in their binary fixed-dose formulations with acceptable accuracy (recovery) and precision.
32650193	7	12	theme	method	1432:1437	arg1	precision					1415:1423	The intra-batch and inter-batch precision	1383:1423	The intra-batch and inter-batch precision of the method	1383:1437	The intra-batch and inter-batch precision of the method was ≤1.64 % while the accuracy was in the range of 96.2-103.3 %.
32650193	7	12	theme	method	1432:1437	arg1	%					1449:1449	≤1.64 %	1443:1449	≤1.64 %	1443:1449	The intra-batch and inter-batch precision of the method was ≤1.64 % while the accuracy was in the range of 96.2-103.3 %.
32650193	0	13	theme	stationary	109:118	arg1	phase					120:124	a polysaccharide stationary phase	92:124	a polysaccharide stationary phase	92:124	Separation of achiral anti-diabetic drugs using sub/supercritical fluid chromatography with a polysaccharide stationary phase: Thermodynamic considerations and molecular docking study.
32650193	1	14	dep	inhibitors	409:418	arg1	inhibitors					409:418	three sodium-glucose co-transporter 2 (SGLT-2) inhibitors	362:418	three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin)	362:467	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	1	14	dep	inhibitors	409:418	arg1	empagliflozin					454:466	empagliflozin	454:466	empagliflozin	454:466	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	1	14	dep	inhibitors	409:418	arg1	dapagliflozin					436:448	dapagliflozin	436:448	dapagliflozin	436:448	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	1	14	dep	inhibitors	409:418	arg1	canagliflozin					421:433	canagliflozin	421:433	canagliflozin	421:433	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	7	15	theme	%	1501:1501	arg1	range					1481:1485	the range	1477:1485	the range of 96.2-103.3 %	1477:1501	The intra-batch and inter-batch precision of the method was ≤1.64 % while the accuracy was in the range of 96.2-103.3 %.
32650193	0	16	theme	Thermodynamic	127:139	arg1	considerations					141:154	Thermodynamic considerations	127:154	Thermodynamic considerations	127:154	Separation of achiral anti-diabetic drugs using sub/supercritical fluid chromatography with a polysaccharide stationary phase: Thermodynamic considerations and molecular docking study.
32650193	1	17	theme	inhibitors	409:418	arg1	determination					331:343	the simultaneous determination	314:343	the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin)	314:467	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	6	18	theme	metformin-canagliflozin	1261:1283	arg1	pairs					1338:1342	metformin-canagliflozin, metformin-dapagliflozin and metformin-empagliflozin pairs	1261:1342	metformin-canagliflozin, metformin-dapagliflozin and metformin-empagliflozin pairs	1261:1342	The chromatographic resolution between metformin-canagliflozin, metformin-dapagliflozin and metformin-empagliflozin pairs was 6.87, 3.44 and 6.47, respectively.
32650193	0	19	with	chromatography	72:85	arg1	phase					120:124	a polysaccharide stationary phase	92:124	a polysaccharide stationary phase	92:124	Separation of achiral anti-diabetic drugs using sub/supercritical fluid chromatography with a polysaccharide stationary phase: Thermodynamic considerations and molecular docking study.
32650193	9	20	theme	stationary	1837:1846	arg1	phase					1848:1852	the chiral stationary phase	1826:1852	the chiral stationary phase	1826:1852	To support the experimental results, molecular docking was performed on Schrodinger software to provide a deeper insight into the interactions between the analytes and the chiral stationary phase and to understand their elution orders under optimized conditions.
32650193	9	21	theme	Schrodinger	1730:1740	arg1	software					1742:1749	Schrodinger software	1730:1749	Schrodinger software	1730:1749	To support the experimental results, molecular docking was performed on Schrodinger software to provide a deeper insight into the interactions between the analytes and the chiral stationary phase and to understand their elution orders under optimized conditions.
32650193	3	22	from	diethylamine	796:807	arg1	v/v					844:846	v/v	844:846	v/v	844:846	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	3	22	from	diethylamine	796:807	arg1	methanol					812:819	methanol	812:819	methanol	812:819	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	3	22	from	diethylamine	796:807	arg1	50:50					837:841	isopropanol 50:50	825:841	isopropanol 50:50 (v/v)	825:847	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	3	22	from	diethylamine	796:807	arg1	ratios					858:863	60:40 ratios	852:863	60:40 ratios	852:863	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	3	23	from	CO2	782:784	arg1	v/v					844:846	v/v	844:846	v/v	844:846	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	3	23	from	CO2	782:784	arg1	methanol					812:819	methanol	812:819	methanol	812:819	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	3	23	from	CO2	782:784	arg1	50:50					837:841	isopropanol 50:50	825:841	isopropanol 50:50 (v/v)	825:847	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	3	23	from	CO2	782:784	arg1	ratios					858:863	60:40 ratios	852:863	60:40 ratios	852:863	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	1	24	theme	fast	195:198	arg1	method					289:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method	185:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method	185:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	2	25	theme	phases	505:510	arg1	impact					474:479	The impact	470:479	The impact of different stationary phases, co-solvents, column temperature and outlet pressure	470:563	The impact of different stationary phases, co-solvents, column temperature and outlet pressure was extensively evaluated for selective separation and quantitation of the drugs.
32650193	8	26	theme	metformin	1535:1543	arg1	inhibitors					1556:1565	metformin and SGLT-2 inhibitors	1535:1565	metformin and SGLT-2 inhibitors in their binary fixed-dose formulations with acceptable accuracy (recovery) and precision	1535:1655	The method was used to analyze metformin and SGLT-2 inhibitors in their binary fixed-dose formulations with acceptable accuracy (recovery) and precision.
32650193	4	27	theme	separation	890:899	arg1	efficiency					901:910	The separation efficiency	886:910	The separation efficiency	886:910	The separation efficiency was mainly dependent on the nature of stationary phase and the mobile phase composition.
32650193	4	27	theme	separation	890:899	arg1	dependent					923:931	dependent	923:931	dependent	923:931	The separation efficiency was mainly dependent on the nature of stationary phase and the mobile phase composition.
32650193	8	28	with	formulations	1594:1605	arg1	recovery					1633:1640	recovery	1633:1640	recovery	1633:1640	The method was used to analyze metformin and SGLT-2 inhibitors in their binary fixed-dose formulations with acceptable accuracy (recovery) and precision.
32650193	8	28	with	formulations	1594:1605	arg1	precision					1647:1655	precision	1647:1655	precision	1647:1655	The method was used to analyze metformin and SGLT-2 inhibitors in their binary fixed-dose formulations with acceptable accuracy (recovery) and precision.
32650193	8	28	with	formulations	1594:1605	arg1	accuracy					1623:1630	acceptable accuracy	1612:1630	acceptable accuracy (recovery)	1612:1641	The method was used to analyze metformin and SGLT-2 inhibitors in their binary fixed-dose formulations with acceptable accuracy (recovery) and precision.
32650193	3	29	theme	0.1	790:792	arg1	%					794:794	%	794:794	%	794:794	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	2	30	theme	stationary	494:503	arg1	co-solvents					513:523	co-solvents	513:523	co-solvents	513:523	The impact of different stationary phases, co-solvents, column temperature and outlet pressure was extensively evaluated for selective separation and quantitation of the drugs.
32650193	2	30	theme	stationary	494:503	arg1	temperature					533:543	column temperature	526:543	column temperature	526:543	The impact of different stationary phases, co-solvents, column temperature and outlet pressure was extensively evaluated for selective separation and quantitation of the drugs.
32650193	2	30	theme	stationary	494:503	arg1	phases					505:510	different stationary phases	484:510	different stationary phases	484:510	The impact of different stationary phases, co-solvents, column temperature and outlet pressure was extensively evaluated for selective separation and quantitation of the drugs.
32650193	2	30	theme	stationary	494:503	arg1	pressure					556:563	outlet pressure	549:563	outlet pressure	549:563	The impact of different stationary phases, co-solvents, column temperature and outlet pressure was extensively evaluated for selective separation and quantitation of the drugs.
32650193	3	31	dep	based	750:754	arg1	amylose					742:748	amylose	742:748	amylose	742:748	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	6	32	theme	chromatographic	1226:1240	arg1	6.87					1348:1351	6.87	1348:1351	6.87	1348:1351	The chromatographic resolution between metformin-canagliflozin, metformin-dapagliflozin and metformin-empagliflozin pairs was 6.87, 3.44 and 6.47, respectively.
32650193	6	32	theme	chromatographic	1226:1240	arg1	resolution					1242:1251	The chromatographic resolution	1222:1251	The chromatographic resolution between metformin-canagliflozin, metformin-dapagliflozin and metformin-empagliflozin pairs	1222:1342	The chromatographic resolution between metformin-canagliflozin, metformin-dapagliflozin and metformin-empagliflozin pairs was 6.87, 3.44 and 6.47, respectively.
32650193	1	33	theme	environmental	204:216	arg1	method					289:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method	185:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method	185:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	0	34	theme	anti-diabetic	22:34	arg1	drugs					36:40	achiral anti-diabetic drugs	14:40	achiral anti-diabetic drugs using sub/supercritical fluid chromatography with a polysaccharide stationary phase	14:124	Separation of achiral anti-diabetic drugs using sub/supercritical fluid chromatography with a polysaccharide stationary phase: Thermodynamic considerations and molecular docking study.
32650193	1	35	theme	simultaneous	318:329	arg1	determination					331:343	the simultaneous determination	314:343	the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin)	314:467	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	4	36	theme	phase	961:965	arg1	nature					940:945	the nature	936:945	the nature of stationary phase	936:965	The separation efficiency was mainly dependent on the nature of stationary phase and the mobile phase composition.
32650193	4	36	theme	phase	961:965	arg1	composition					988:998	the mobile phase composition	971:998	the mobile phase composition	971:998	The separation efficiency was mainly dependent on the nature of stationary phase and the mobile phase composition.
32650193	7	37	theme	inter-batch	1403:1413	arg1	precision					1415:1423	The intra-batch and inter-batch precision	1383:1423	The intra-batch and inter-batch precision of the method	1383:1437	The intra-batch and inter-batch precision of the method was ≤1.64 % while the accuracy was in the range of 96.2-103.3 %.
32650193	7	37	theme	inter-batch	1403:1413	arg1	%					1449:1449	≤1.64 %	1443:1449	≤1.64 %	1443:1449	The intra-batch and inter-batch precision of the method was ≤1.64 % while the accuracy was in the range of 96.2-103.3 %.
32650193	5	38	theme	quantitation	1034:1045	arg1	limit					1005:1009	The limit	1001:1009	The limit of detection (LOD) and quantitation (LOQ) of the method	1001:1065	The limit of detection (LOD) and quantitation (LOQ) of the method were 0.155 and 0.469, 0.062 and 0.187, 0.015 and 0.045, 0.028 and 0.084 μg/mL for metformin, canagliflozin, dapagliflozin and empagliflozin, respectively.
32650193	5	38	theme	quantitation	1034:1045	arg1	0.155					1072:1076	0.155	1072:1076	0.155	1072:1076	The limit of detection (LOD) and quantitation (LOQ) of the method were 0.155 and 0.469, 0.062 and 0.187, 0.015 and 0.045, 0.028 and 0.084 μg/mL for metformin, canagliflozin, dapagliflozin and empagliflozin, respectively.
32650193	1	39	theme	friendly	218:225	arg1	method					289:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method	185:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method	185:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	0	40	theme	achiral	14:20	arg1	drugs					36:40	achiral anti-diabetic drugs	14:40	achiral anti-diabetic drugs using sub/supercritical fluid chromatography with a polysaccharide stationary phase	14:124	Separation of achiral anti-diabetic drugs using sub/supercritical fluid chromatography with a polysaccharide stationary phase: Thermodynamic considerations and molecular docking study.
32650193	3	41	theme	%	794:794	arg1	CO2					782:784	CO2	782:784	CO2	782:784	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	3	41	theme	%	794:794	arg1	phase					879:883	the mobile phase	868:883	the mobile phase	868:883	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	3	41	theme	%	794:794	arg1	diethylamine					796:807	0.1 % diethylamine	790:807	0.1 % diethylamine	790:807	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	4	42	theme	stationary	950:959	arg1	phase					961:965	stationary phase	950:965	stationary phase	950:965	The separation efficiency was mainly dependent on the nature of stationary phase and the mobile phase composition.
32650193	3	43	theme	best	712:715	arg1	results					717:723	the best results	708:723	the best results	708:723	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	1	44	theme	simple	187:192	arg1	method					289:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method	185:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method	185:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	1	45	theme	supercritical	227:239	arg1	method					289:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method	185:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method	185:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	2	46	theme	column	526:531	arg1	temperature					533:543	column temperature	526:543	column temperature	526:543	The impact of different stationary phases, co-solvents, column temperature and outlet pressure was extensively evaluated for selective separation and quantitation of the drugs.
32650193	2	46	theme	column	526:531	arg1	phases					505:510	different stationary phases	484:510	different stationary phases	484:510	The impact of different stationary phases, co-solvents, column temperature and outlet pressure was extensively evaluated for selective separation and quantitation of the drugs.
32650193	0	47	theme	docking	170:176	arg1	study					178:182	molecular docking study	160:182	molecular docking study	160:182	Separation of achiral anti-diabetic drugs using sub/supercritical fluid chromatography with a polysaccharide stationary phase: Thermodynamic considerations and molecular docking study.
32650193	8	48	used	used	1519:1522	arg2	method					1508:1513	The method	1504:1513	The method	1504:1513	The method was used to analyze metformin and SGLT-2 inhibitors in their binary fixed-dose formulations with acceptable accuracy (recovery) and precision.
32650193	3	49	theme	achiral	663:669	arg1	phases					693:698	several achiral and chiral stationary phases	655:698	several achiral and chiral stationary phases tested	655:705	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	1	50	theme	fluid	241:245	arg1	method					289:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method	185:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method	185:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	0	51	theme	drugs	36:40	arg1	Separation					0:9	Separation	0:9	Separation of achiral anti-diabetic drugs using sub/supercritical fluid chromatography with a polysaccharide stationary phase	0:124	Separation of achiral anti-diabetic drugs using sub/supercritical fluid chromatography with a polysaccharide stationary phase: Thermodynamic considerations and molecular docking study.
32650193	1	52	theme	metformin	348:356	arg1	determination					331:343	the simultaneous determination	314:343	the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin)	314:467	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	0	53	theme	molecular	160:168	arg1	study					178:182	molecular docking study	160:182	molecular docking study	160:182	Separation of achiral anti-diabetic drugs using sub/supercritical fluid chromatography with a polysaccharide stationary phase: Thermodynamic considerations and molecular docking study.
32650193	2	54	theme	different	484:492	arg1	co-solvents					513:523	co-solvents	513:523	co-solvents	513:523	The impact of different stationary phases, co-solvents, column temperature and outlet pressure was extensively evaluated for selective separation and quantitation of the drugs.
32650193	2	54	theme	different	484:492	arg1	temperature					533:543	column temperature	526:543	column temperature	526:543	The impact of different stationary phases, co-solvents, column temperature and outlet pressure was extensively evaluated for selective separation and quantitation of the drugs.
32650193	2	54	theme	different	484:492	arg1	phases					505:510	different stationary phases	484:510	different stationary phases	484:510	The impact of different stationary phases, co-solvents, column temperature and outlet pressure was extensively evaluated for selective separation and quantitation of the drugs.
32650193	2	54	theme	different	484:492	arg1	pressure					556:563	outlet pressure	549:563	outlet pressure	549:563	The impact of different stationary phases, co-solvents, column temperature and outlet pressure was extensively evaluated for selective separation and quantitation of the drugs.
32650193	2	55	theme	selective	595:603	arg1	separation					605:614	selective separation	595:614	selective separation	595:614	The impact of different stationary phases, co-solvents, column temperature and outlet pressure was extensively evaluated for selective separation and quantitation of the drugs.
32650193	1	56	theme	chromatography-photodiode	247:271	arg1	method					289:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method	185:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method	185:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	0	57	theme	fluid	66:70	arg1	chromatography					72:85	sub/supercritical fluid chromatography	48:85	sub/supercritical fluid chromatography with a polysaccharide stationary phase	48:124	Separation of achiral anti-diabetic drugs using sub/supercritical fluid chromatography with a polysaccharide stationary phase: Thermodynamic considerations and molecular docking study.
32650193	8	58	theme	fixed-dose	1583:1592	arg1	formulations					1594:1605	their binary fixed-dose formulations	1570:1605	their binary fixed-dose formulations with acceptable accuracy (recovery) and precision	1570:1655	The method was used to analyze metformin and SGLT-2 inhibitors in their binary fixed-dose formulations with acceptable accuracy (recovery) and precision.
32650193	8	59	theme	SGLT-2	1549:1554	arg1	inhibitors					1556:1565	metformin and SGLT-2 inhibitors	1535:1565	metformin and SGLT-2 inhibitors in their binary fixed-dose formulations with acceptable accuracy (recovery) and precision	1535:1655	The method was used to analyze metformin and SGLT-2 inhibitors in their binary fixed-dose formulations with acceptable accuracy (recovery) and precision.
32650193	5	60	theme	0.045	1116:1120	arg1	μg/mL					1139:1143	0.045, 0.028 and 0.084 μg/mL	1116:1143	μg/mL	1139:1143	The limit of detection (LOD) and quantitation (LOQ) of the method were 0.155 and 0.469, 0.062 and 0.187, 0.015 and 0.045, 0.028 and 0.084 μg/mL for metformin, canagliflozin, dapagliflozin and empagliflozin, respectively.
32650193	0	61	theme	sub/supercritical	48:64	arg1	chromatography					72:85	sub/supercritical fluid chromatography	48:85	sub/supercritical fluid chromatography with a polysaccharide stationary phase	48:124	Separation of achiral anti-diabetic drugs using sub/supercritical fluid chromatography with a polysaccharide stationary phase: Thermodynamic considerations and molecular docking study.
32650193	5	62	theme	0.084	1133:1137	arg1	μg/mL					1139:1143	0.045, 0.028 and 0.084 μg/mL	1116:1143	μg/mL	1139:1143	The limit of detection (LOD) and quantitation (LOQ) of the method were 0.155 and 0.469, 0.062 and 0.187, 0.015 and 0.045, 0.028 and 0.084 μg/mL for metformin, canagliflozin, dapagliflozin and empagliflozin, respectively.
32650193	2	63	theme	drugs	640:644	arg1	quantitation					620:631	quantitation	620:631	quantitation	620:631	The impact of different stationary phases, co-solvents, column temperature and outlet pressure was extensively evaluated for selective separation and quantitation of the drugs.
32650193	2	63	theme	drugs	640:644	arg1	separation					605:614	selective separation	595:614	selective separation	595:614	The impact of different stationary phases, co-solvents, column temperature and outlet pressure was extensively evaluated for selective separation and quantitation of the drugs.
32650193	3	64	theme	several	655:661	arg1	phases					693:698	several achiral and chiral stationary phases	655:698	several achiral and chiral stationary phases tested	655:705	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	9	65	theme	experimental	1673:1684	arg1	results					1686:1692	the experimental results	1669:1692	the experimental results	1669:1692	To support the experimental results, molecular docking was performed on Schrodinger software to provide a deeper insight into the interactions between the analytes and the chiral stationary phase and to understand their elution orders under optimized conditions.
32650193	3	66	theme	IG	766:767	arg1	column					769:774	amylose based Chiralpak IG column	742:774	amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase	742:883	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	1	67	theme	sodium-glucose	368:381	arg1	inhibitors					409:418	three sodium-glucose co-transporter 2 (SGLT-2) inhibitors	362:418	three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin)	362:467	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	1	67	theme	sodium-glucose	368:381	arg1	empagliflozin					454:466	empagliflozin	454:466	empagliflozin	454:466	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	1	67	theme	sodium-glucose	368:381	arg1	dapagliflozin					436:448	dapagliflozin	436:448	dapagliflozin	436:448	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	1	67	theme	sodium-glucose	368:381	arg1	canagliflozin					421:433	canagliflozin	421:433	canagliflozin	421:433	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	9	68	theme	elution	1878:1884	arg1	orders					1886:1891	their elution orders	1872:1891	their elution orders	1872:1891	To support the experimental results, molecular docking was performed on Schrodinger software to provide a deeper insight into the interactions between the analytes and the chiral stationary phase and to understand their elution orders under optimized conditions.
32650193	1	69	theme	array	273:277	arg1	method					289:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method	185:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method	185:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	3	70	theme	isopropanol	825:835	arg1	v/v					844:846	v/v	844:846	v/v	844:846	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	3	70	theme	isopropanol	825:835	arg1	50:50					837:841	isopropanol 50:50	825:841	isopropanol 50:50 (v/v)	825:847	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	5	71	theme	method	1060:1065	arg1	LOQ					1048:1050	LOQ	1048:1050	LOQ	1048:1050	The limit of detection (LOD) and quantitation (LOQ) of the method were 0.155 and 0.469, 0.062 and 0.187, 0.015 and 0.045, 0.028 and 0.084 μg/mL for metformin, canagliflozin, dapagliflozin and empagliflozin, respectively.
32650193	5	71	theme	method	1060:1065	arg1	LOD					1025:1027	LOD	1025:1027	LOD	1025:1027	The limit of detection (LOD) and quantitation (LOQ) of the method were 0.155 and 0.469, 0.062 and 0.187, 0.015 and 0.045, 0.028 and 0.084 μg/mL for metformin, canagliflozin, dapagliflozin and empagliflozin, respectively.
32650193	5	71	theme	method	1060:1065	arg1	quantitation					1034:1045	quantitation	1034:1045	quantitation (LOQ)	1034:1051	The limit of detection (LOD) and quantitation (LOQ) of the method were 0.155 and 0.469, 0.062 and 0.187, 0.015 and 0.045, 0.028 and 0.084 μg/mL for metformin, canagliflozin, dapagliflozin and empagliflozin, respectively.
32650193	5	71	theme	method	1060:1065	arg1	detection					1014:1022	detection	1014:1022	detection (LOD)	1014:1028	The limit of detection (LOD) and quantitation (LOQ) of the method were 0.155 and 0.469, 0.062 and 0.187, 0.015 and 0.045, 0.028 and 0.084 μg/mL for metformin, canagliflozin, dapagliflozin and empagliflozin, respectively.
32650193	7	72	theme	intra-batch	1387:1397	arg1	precision					1415:1423	The intra-batch and inter-batch precision	1383:1423	The intra-batch and inter-batch precision of the method	1383:1437	The intra-batch and inter-batch precision of the method was ≤1.64 % while the accuracy was in the range of 96.2-103.3 %.
32650193	7	72	theme	intra-batch	1387:1397	arg1	%					1449:1449	≤1.64 %	1443:1449	≤1.64 %	1443:1449	The intra-batch and inter-batch precision of the method was ≤1.64 % while the accuracy was in the range of 96.2-103.3 %.
32650193	3	73	theme	60:40	852:856	arg1	ratios					858:863	60:40 ratios	852:863	60:40 ratios	852:863	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	9	74	theme	deeper	1764:1769	arg1	insight					1771:1777	a deeper insight	1762:1777	a deeper insight into the interactions between the analytes and the chiral stationary phase	1762:1852	To support the experimental results, molecular docking was performed on Schrodinger software to provide a deeper insight into the interactions between the analytes and the chiral stationary phase and to understand their elution orders under optimized conditions.
32650193	3	75	theme	chiral	675:680	arg1	phases					693:698	several achiral and chiral stationary phases	655:698	several achiral and chiral stationary phases tested	655:705	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	9	76	theme	optimized	1899:1907	arg1	conditions					1909:1918	optimized conditions	1899:1918	optimized conditions	1899:1918	To support the experimental results, molecular docking was performed on Schrodinger software to provide a deeper insight into the interactions between the analytes and the chiral stationary phase and to understand their elution orders under optimized conditions.
32650193	1	77	theme	SFC-PDA	280:286	arg1	method					289:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method	185:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method	185:294	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	2	78	theme	outlet	549:554	arg1	phases					505:510	different stationary phases	484:510	different stationary phases	484:510	The impact of different stationary phases, co-solvents, column temperature and outlet pressure was extensively evaluated for selective separation and quantitation of the drugs.
32650193	2	78	theme	outlet	549:554	arg1	pressure					556:563	outlet pressure	549:563	outlet pressure	549:563	The impact of different stationary phases, co-solvents, column temperature and outlet pressure was extensively evaluated for selective separation and quantitation of the drugs.
32650193	1	79	theme	co-transporter	383:396	arg1	inhibitors					409:418	three sodium-glucose co-transporter 2 (SGLT-2) inhibitors	362:418	three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin)	362:467	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	1	79	theme	co-transporter	383:396	arg1	empagliflozin					454:466	empagliflozin	454:466	empagliflozin	454:466	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	1	79	theme	co-transporter	383:396	arg1	dapagliflozin					436:448	dapagliflozin	436:448	dapagliflozin	436:448	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	1	79	theme	co-transporter	383:396	arg1	canagliflozin					421:433	canagliflozin	421:433	canagliflozin	421:433	A simple, fast and environmental friendly supercritical fluid chromatography-photodiode array (SFC-PDA) method was developed for the simultaneous determination of metformin and three sodium-glucose co-transporter 2 (SGLT-2) inhibitors (canagliflozin, dapagliflozin and empagliflozin).
32650193	3	80	theme	stationary	682:691	arg1	phases					693:698	several achiral and chiral stationary phases	655:698	several achiral and chiral stationary phases tested	655:705	Amongst several achiral and chiral stationary phases tested, the best results were obtained on amylose based Chiralpak IG column using CO2 and 0.1 % diethylamine in methanol and isopropanol 50:50 (v/v) in 60:40 ratios as the mobile phase.
32650193	5	81	theme	detection	1014:1022	arg1	limit					1005:1009	The limit	1001:1009	The limit of detection (LOD) and quantitation (LOQ) of the method	1001:1065	The limit of detection (LOD) and quantitation (LOQ) of the method were 0.155 and 0.469, 0.062 and 0.187, 0.015 and 0.045, 0.028 and 0.084 μg/mL for metformin, canagliflozin, dapagliflozin and empagliflozin, respectively.
32650193	5	81	theme	detection	1014:1022	arg1	0.155					1072:1076	0.155	1072:1076	0.155	1072:1076	The limit of detection (LOD) and quantitation (LOQ) of the method were 0.155 and 0.469, 0.062 and 0.187, 0.015 and 0.045, 0.028 and 0.084 μg/mL for metformin, canagliflozin, dapagliflozin and empagliflozin, respectively.
32650193	8	82	from	inhibitors	1556:1565	arg1	formulations					1594:1605	their binary fixed-dose formulations	1570:1605	their binary fixed-dose formulations with acceptable accuracy (recovery) and precision	1570:1655	The method was used to analyze metformin and SGLT-2 inhibitors in their binary fixed-dose formulations with acceptable accuracy (recovery) and precision.
32650193	4	83	theme	mobile	975:980	arg1	composition					988:998	the mobile phase composition	971:998	the mobile phase composition	971:998	The separation efficiency was mainly dependent on the nature of stationary phase and the mobile phase composition.
34288919	0	0	theme	microbiota	83:92	arg1	capabilities					45:56	the fermentation capabilities	28:56	the fermentation capabilities of the healthy human gut microbiota	28:92	Functional heterogeneity in the fermentation capabilities of the healthy human gut microbiota.
34288919	1	1	theme	heterogeneous	144:156	arg1	composition					158:168	its highly heterogeneous composition	133:168	its highly heterogeneous composition across different individuals	133:197	The human gut microbiota is known for its highly heterogeneous composition across different individuals.
34288919	0	2	theme	gut	79:81	arg1	microbiota					83:92	the healthy human gut microbiota	61:92	the healthy human gut microbiota	61:92	Functional heterogeneity in the fermentation capabilities of the healthy human gut microbiota.
34288919	0	3	from	heterogeneity	11:23	arg1	capabilities					45:56	the fermentation capabilities	28:56	the fermentation capabilities of the healthy human gut microbiota	28:92	Functional heterogeneity in the fermentation capabilities of the healthy human gut microbiota.
34288919	4	4	theme	MMPs	678:681	arg1	aspects					661:667	aspects	661:667	aspects of these MMPs	661:681	We also show that aspects of these MMPs can be predicted from composition using 16S rRNA sequencing.
34288919	7	5	from	production	1353:1362	arg1	individual					1375:1384	a given individual	1367:1384	a given individual	1367:1384	Though in vivo studies are required to confirm these data in the context of the gut, in addition to in vivo read outs of SCFAs produced in response to specific fiber spike-ins, these data suggest that optimizing SCFA production in a given individual through targeted fiber supplementation requires quantitative understanding of their MMP.
34288919	7	6	theme	fiber	1403:1407	arg1	supplementation					1409:1423	targeted fiber supplementation	1394:1423	targeted fiber supplementation	1394:1423	Though in vivo studies are required to confirm these data in the context of the gut, in addition to in vivo read outs of SCFAs produced in response to specific fiber spike-ins, these data suggest that optimizing SCFA production in a given individual through targeted fiber supplementation requires quantitative understanding of their MMP.
34288919	7	7	theme	specific	1287:1294	arg1	spike-ins					1302:1310	specific fiber spike-ins	1287:1310	specific fiber spike-ins	1287:1310	Though in vivo studies are required to confirm these data in the context of the gut, in addition to in vivo read outs of SCFAs produced in response to specific fiber spike-ins, these data suggest that optimizing SCFA production in a given individual through targeted fiber supplementation requires quantitative understanding of their MMP.
34288919	3	8	theme	different	549:557	arg1	quantities					559:568	different quantities	549:568	different quantities	549:568	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	3	9	theme	proportions	574:584	arg1	production					535:544	the production	531:544	the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs	531:640	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	3	10	from	inputs	635:640	arg1	production					535:544	the production	531:544	the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs	531:640	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	2	11	theme	complex	291:297	arg1	polysaccharides					299:313	complex polysaccharides	291:313	complex polysaccharides	291:313	However, relatively little is known about functional differences in its ability to ferment complex polysaccharides.
34288919	3	12	theme	quantities	559:568	arg1	production					535:544	the production	531:544	the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs	531:640	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	6	13	theme	absorbed	1071:1078	arg1	SCFA					1080:1083	absorbed SCFA	1071:1083	absorbed SCFA	1071:1083	We leverage our ex vivo data to construct a model of SCFA production and absorption in vivo, and argue that inter-individual differences in quantities of absorbed SCFA are directly related to differences in production.
34288919	3	14	dep	ex	324:325	arg1	vivo					327:330	vivo	327:330	vivo	327:330	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	6	15	theme	inter-individual	1025:1040	arg1	differences					1042:1052	inter-individual differences	1025:1052	inter-individual differences in quantities of absorbed SCFA	1025:1083	We leverage our ex vivo data to construct a model of SCFA production and absorption in vivo, and argue that inter-individual differences in quantities of absorbed SCFA are directly related to differences in production.
34288919	7	16	theme	optimizing	1337:1346	arg1	production					1353:1362	optimizing SCFA production	1337:1362	optimizing SCFA production in a given individual through targeted fiber supplementation	1337:1423	Though in vivo studies are required to confirm these data in the context of the gut, in addition to in vivo read outs of SCFAs produced in response to specific fiber spike-ins, these data suggest that optimizing SCFA production in a given individual through targeted fiber supplementation requires quantitative understanding of their MMP.
34288919	7	17	dep	spike-ins	1302:1310	arg1	response					1275:1282	response	1275:1282	response	1275:1282	Though in vivo studies are required to confirm these data in the context of the gut, in addition to in vivo read outs of SCFAs produced in response to specific fiber spike-ins, these data suggest that optimizing SCFA production in a given individual through targeted fiber supplementation requires quantitative understanding of their MMP.
34288919	5	18	theme	ingested	833:840	arg1	bolus					842:846	an ingested bolus	830:846	an ingested bolus of fiber	830:855	From experiments performed using the same dietary fibers in vivo, we demonstrate that an ingested bolus of fiber is almost entirely consumed by the microbiota upon passage.
34288919	6	19	theme	SCFA	1080:1083	arg1	quantities					1057:1066	quantities	1057:1066	quantities of absorbed SCFA	1057:1083	We leverage our ex vivo data to construct a model of SCFA production and absorption in vivo, and argue that inter-individual differences in quantities of absorbed SCFA are directly related to differences in production.
34288919	7	20	theme	SCFA	1348:1351	arg1	production					1353:1362	optimizing SCFA production	1337:1362	optimizing SCFA production in a given individual through targeted fiber supplementation	1337:1423	Though in vivo studies are required to confirm these data in the context of the gut, in addition to in vivo read outs of SCFAs produced in response to specific fiber spike-ins, these data suggest that optimizing SCFA production in a given individual through targeted fiber supplementation requires quantitative understanding of their MMP.
34288919	1	21	theme	different	177:185	arg1	individuals					187:197	different individuals	177:197	different individuals	177:197	The human gut microbiota is known for its highly heterogeneous composition across different individuals.
34288919	7	22	theme	given	1369:1373	arg1	individual					1375:1384	a given individual	1367:1384	a given individual	1367:1384	Though in vivo studies are required to confirm these data in the context of the gut, in addition to in vivo read outs of SCFAs produced in response to specific fiber spike-ins, these data suggest that optimizing SCFA production in a given individual through targeted fiber supplementation requires quantitative understanding of their MMP.
34288919	3	23	theme	healthy	350:356	arg1	donors					364:369	healthy human donors	350:369	healthy human donors	350:369	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	4	24	theme	16S	723:725	arg1	sequencing					732:741	16S rRNA sequencing	723:741	16S rRNA sequencing	723:741	We also show that aspects of these MMPs can be predicted from composition using 16S rRNA sequencing.
34288919	7	25	theme	gut	1216:1218	arg1	context					1201:1207	the context	1197:1207	the context of the gut	1197:1218	Though in vivo studies are required to confirm these data in the context of the gut, in addition to in vivo read outs of SCFAs produced in response to specific fiber spike-ins, these data suggest that optimizing SCFA production in a given individual through targeted fiber supplementation requires quantitative understanding of their MMP.
34288919	7	26	theme	read	1244:1247	arg1	outs					1249:1252	in vivo read outs	1236:1252	in vivo read outs of SCFAs produced in response to specific fiber spike-ins	1236:1310	Though in vivo studies are required to confirm these data in the context of the gut, in addition to in vivo read outs of SCFAs produced in response to specific fiber spike-ins, these data suggest that optimizing SCFA production in a given individual through targeted fiber supplementation requires quantitative understanding of their MMP.
34288919	0	27	theme	Functional	0:9	arg1	heterogeneity					11:23	Functional heterogeneity	0:23	Functional heterogeneity in the fermentation capabilities of the healthy human gut microbiota	0:92	Functional heterogeneity in the fermentation capabilities of the healthy human gut microbiota.
34288919	3	28	theme	Short	589:593	arg1	Acids					607:611	Short Chain Fatty Acids	589:611	Short Chain Fatty Acids (SCFAs)	589:619	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	3	28	theme	Short	589:593	arg1	SCFAs					614:618	SCFAs	614:618	SCFAs	614:618	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	5	29	theme	fiber	851:855	arg1	bolus					842:846	an ingested bolus	830:846	an ingested bolus of fiber	830:855	From experiments performed using the same dietary fibers in vivo, we demonstrate that an ingested bolus of fiber is almost entirely consumed by the microbiota upon passage.
34288919	7	30	theme	fiber	1296:1300	arg1	spike-ins					1302:1310	specific fiber spike-ins	1287:1310	specific fiber spike-ins	1287:1310	Though in vivo studies are required to confirm these data in the context of the gut, in addition to in vivo read outs of SCFAs produced in response to specific fiber spike-ins, these data suggest that optimizing SCFA production in a given individual through targeted fiber supplementation requires quantitative understanding of their MMP.
34288919	1	31	theme	human	99:103	arg1	microbiota					109:118	The human gut microbiota	95:118	The human gut microbiota	95:118	The human gut microbiota is known for its highly heterogeneous composition across different individuals.
34288919	3	32	theme	Acids	607:611	arg1	proportions					574:584	proportions	574:584	proportions of Short Chain Fatty Acids (SCFAs)	574:619	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	3	32	theme	Acids	607:611	arg1	quantities					559:568	different quantities	549:568	different quantities	549:568	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	6	33	theme	ex	933:934	arg1	data					941:944	our ex vivo data	929:944	our ex vivo data	929:944	We leverage our ex vivo data to construct a model of SCFA production and absorption in vivo, and argue that inter-individual differences in quantities of absorbed SCFA are directly related to differences in production.
34288919	3	34	from	donors	364:369	arg1	measurements					332:343	ex vivo measurements	324:343	ex vivo measurements from healthy human donors	324:369	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	1	35	theme	gut	105:107	arg1	microbiota					109:118	The human gut microbiota	95:118	The human gut microbiota	95:118	The human gut microbiota is known for its highly heterogeneous composition across different individuals.
34288919	3	36	theme	microbial	420:428	arg1	MMPs					452:455	MMPs	452:455	MMPs	452:455	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	3	36	theme	microbial	420:428	arg1	phenotypes					440:449	microbial metabolic phenotypes	420:449	their microbial metabolic phenotypes (MMPs)	414:456	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	7	37	theme	in	1143:1144	arg1	studies					1151:1157	in vivo studies	1143:1157	in vivo studies	1143:1157	Though in vivo studies are required to confirm these data in the context of the gut, in addition to in vivo read outs of SCFAs produced in response to specific fiber spike-ins, these data suggest that optimizing SCFA production in a given individual through targeted fiber supplementation requires quantitative understanding of their MMP.
34288919	2	38	theme	functional	242:251	arg1	differences					253:263	functional differences	242:263	functional differences in its ability to ferment complex polysaccharides	242:313	However, relatively little is known about functional differences in its ability to ferment complex polysaccharides.
34288919	6	39	from	differences	1109:1119	arg1	production					1124:1133	production	1124:1133	production	1124:1133	We leverage our ex vivo data to construct a model of SCFA production and absorption in vivo, and argue that inter-individual differences in quantities of absorbed SCFA are directly related to differences in production.
34288919	0	40	theme	fermentation	32:43	arg1	capabilities					45:56	the fermentation capabilities	28:56	the fermentation capabilities of the healthy human gut microbiota	28:92	Functional heterogeneity in the fermentation capabilities of the healthy human gut microbiota.
34288919	7	41	theme	targeted	1394:1401	arg1	supplementation					1409:1423	targeted fiber supplementation	1394:1423	targeted fiber supplementation	1394:1423	Though in vivo studies are required to confirm these data in the context of the gut, in addition to in vivo read outs of SCFAs produced in response to specific fiber spike-ins, these data suggest that optimizing SCFA production in a given individual through targeted fiber supplementation requires quantitative understanding of their MMP.
34288919	3	42	theme	metabolic	430:438	arg1	MMPs					452:455	MMPs	452:455	MMPs	452:455	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	3	42	theme	metabolic	430:438	arg1	phenotypes					440:449	microbial metabolic phenotypes	420:449	their microbial metabolic phenotypes (MMPs)	414:456	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	3	43	theme	same	630:633	arg1	inputs					635:640	the same inputs	626:640	the same inputs	626:640	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	7	44	dep	outs	1249:1252	arg1	addition					1224:1231	addition	1224:1231	addition	1224:1231	Though in vivo studies are required to confirm these data in the context of the gut, in addition to in vivo read outs of SCFAs produced in response to specific fiber spike-ins, these data suggest that optimizing SCFA production in a given individual through targeted fiber supplementation requires quantitative understanding of their MMP.
34288919	6	45	theme	absorption	990:999	arg1	model					961:965	a model	959:965	a model of SCFA production and absorption	959:999	We leverage our ex vivo data to construct a model of SCFA production and absorption in vivo, and argue that inter-individual differences in quantities of absorbed SCFA are directly related to differences in production.
34288919	5	46	theme	same	781:784	arg1	fibers					794:799	the same dietary fibers	777:799	the same dietary fibers	777:799	From experiments performed using the same dietary fibers in vivo, we demonstrate that an ingested bolus of fiber is almost entirely consumed by the microbiota upon passage.
34288919	3	47	theme	ex	324:325	arg1	measurements					332:343	ex vivo measurements	324:343	ex vivo measurements from healthy human donors	324:369	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	3	48	theme	human	358:362	arg1	donors					364:369	healthy human donors	350:369	healthy human donors	350:369	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	3	49	theme	Chain	595:599	arg1	Acids					607:611	Short Chain Fatty Acids	589:611	Short Chain Fatty Acids (SCFAs)	589:619	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	3	49	theme	Chain	595:599	arg1	SCFAs					614:618	SCFAs	614:618	SCFAs	614:618	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	5	50	theme	dietary	786:792	arg1	fibers					794:799	the same dietary fibers	777:799	the same dietary fibers	777:799	From experiments performed using the same dietary fibers in vivo, we demonstrate that an ingested bolus of fiber is almost entirely consumed by the microbiota upon passage.
34288919	6	51	dep	ex	933:934	arg1	vivo					936:939	vivo	936:939	vivo	936:939	We leverage our ex vivo data to construct a model of SCFA production and absorption in vivo, and argue that inter-individual differences in quantities of absorbed SCFA are directly related to differences in production.
34288919	3	52	theme	Fatty	601:605	arg1	Acids					607:611	Short Chain Fatty Acids	589:611	Short Chain Fatty Acids (SCFAs)	589:619	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	3	52	theme	Fatty	601:605	arg1	SCFAs					614:618	SCFAs	614:618	SCFAs	614:618	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	3	53	from	differences	469:479	arg1	composition					501:511	their microbiota composition	484:511	their microbiota composition	484:511	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	7	54	theme	MMP	1470:1472	arg1	understanding					1447:1459	quantitative understanding	1434:1459	quantitative understanding of their MMP	1434:1472	Though in vivo studies are required to confirm these data in the context of the gut, in addition to in vivo read outs of SCFAs produced in response to specific fiber spike-ins, these data suggest that optimizing SCFA production in a given individual through targeted fiber supplementation requires quantitative understanding of their MMP.
34288919	7	55	dep	in	1143:1144	arg1	vivo					1146:1149	vivo	1146:1149	vivo	1146:1149	Though in vivo studies are required to confirm these data in the context of the gut, in addition to in vivo read outs of SCFAs produced in response to specific fiber spike-ins, these data suggest that optimizing SCFA production in a given individual through targeted fiber supplementation requires quantitative understanding of their MMP.
34288919	2	56	from	differences	253:263	arg1	ability					272:278	its ability to ferment complex polysaccharides	268:313	its ability to ferment complex polysaccharides	268:313	However, relatively little is known about functional differences in its ability to ferment complex polysaccharides.
34288919	7	57	theme	quantitative	1434:1445	arg1	understanding					1447:1459	quantitative understanding	1434:1459	quantitative understanding of their MMP	1434:1472	Though in vivo studies are required to confirm these data in the context of the gut, in addition to in vivo read outs of SCFAs produced in response to specific fiber spike-ins, these data suggest that optimizing SCFA production in a given individual through targeted fiber supplementation requires quantitative understanding of their MMP.
34288919	4	58	theme	rRNA	727:730	arg1	sequencing					732:741	16S rRNA sequencing	723:741	16S rRNA sequencing	723:741	We also show that aspects of these MMPs can be predicted from composition using 16S rRNA sequencing.
34288919	3	59	theme	microbiota	490:499	arg1	composition					501:511	their microbiota composition	484:511	their microbiota composition	484:511	Through ex vivo measurements from healthy human donors, we show that individuals vary markedly in their microbial metabolic phenotypes (MMPs), mirroring differences in their microbiota composition, and resulting in the production of different quantities and proportions of Short Chain Fatty Acids (SCFAs) from the same inputs.
34288919	6	60	theme	production	975:984	arg1	model					961:965	a model	959:965	a model of SCFA production and absorption	959:999	We leverage our ex vivo data to construct a model of SCFA production and absorption in vivo, and argue that inter-individual differences in quantities of absorbed SCFA are directly related to differences in production.
34288919	0	61	theme	human	73:77	arg1	microbiota					83:92	the healthy human gut microbiota	61:92	the healthy human gut microbiota	61:92	Functional heterogeneity in the fermentation capabilities of the healthy human gut microbiota.
34288919	6	62	from	differences	1042:1052	arg1	quantities					1057:1066	quantities	1057:1066	quantities of absorbed SCFA	1057:1083	We leverage our ex vivo data to construct a model of SCFA production and absorption in vivo, and argue that inter-individual differences in quantities of absorbed SCFA are directly related to differences in production.
34288919	7	63	theme	SCFAs	1257:1261	arg1	outs					1249:1252	in vivo read outs	1236:1252	in vivo read outs of SCFAs produced in response to specific fiber spike-ins	1236:1310	Though in vivo studies are required to confirm these data in the context of the gut, in addition to in vivo read outs of SCFAs produced in response to specific fiber spike-ins, these data suggest that optimizing SCFA production in a given individual through targeted fiber supplementation requires quantitative understanding of their MMP.
34288919	6	64	theme	SCFA	970:973	arg1	production					975:984	SCFA production	970:984	SCFA production	970:984	We leverage our ex vivo data to construct a model of SCFA production and absorption in vivo, and argue that inter-individual differences in quantities of absorbed SCFA are directly related to differences in production.
34288919	0	65	theme	healthy	65:71	arg1	microbiota					83:92	the healthy human gut microbiota	61:92	the healthy human gut microbiota	61:92	Functional heterogeneity in the fermentation capabilities of the healthy human gut microbiota.
32204032	5	0	theme	mechanical	543:552	arg1	properties					554:563	the mechanical properties	539:563	the mechanical properties of the composite film (34 MPa)	539:594	Further, the addition of sodium alginate improved the mechanical properties of the composite film (34 MPa).
32204032	3	1	theme	water	346:350	arg1	barrier					358:364	water vapor barrier	346:364	water vapor barrier	346:364	This composite film exhibited an excellent film-forming ability and water vapor barrier.
32204032	7	2	from	preparation	778:788	arg1	future					835:840	future	835:840	future	835:840	This study can provide an essential guideline for the preparation of antibacterial food packaging films in the future.
32204032	4	3	theme	visible	416:422	arg1	region					424:429	the visible region	412:429	the visible region	412:429	In addition, with 11% light transmittance in the visible region, this film exhibited worthwhile light barrier properties.
32204032	1	4	theme	food	185:188	arg1	transportation					167:180	transportation	167:180	transportation	167:180	The preparation of an antibacterial film is imperative for the preservation and transportation of food.
32204032	1	4	theme	food	185:188	arg1	preservation					150:161	preservation	150:161	preservation	150:161	The preparation of an antibacterial film is imperative for the preservation and transportation of food.
32204032	4	5	theme	11	385:386	arg1	%					387:387	%	387:387	%	387:387	In addition, with 11% light transmittance in the visible region, this film exhibited worthwhile light barrier properties.
32204032	4	6	from	transmittance	395:407	arg1	region					424:429	the visible region	412:429	the visible region	412:429	In addition, with 11% light transmittance in the visible region, this film exhibited worthwhile light barrier properties.
32204032	6	7	theme	amino	608:612	arg1	groups					614:619	The active amino groups	597:619	The active amino groups on the pullulan derivative backbone	597:655	The active amino groups on the pullulan derivative backbone induced antimicrobial activity against both E. coli and S. aureus.
32204032	5	8	theme	sodium	514:519	arg1	alginate					521:528	sodium alginate	514:528	sodium alginate	514:528	Further, the addition of sodium alginate improved the mechanical properties of the composite film (34 MPa).
32204032	6	9	theme	active	601:606	arg1	groups					614:619	The active amino groups	597:619	The active amino groups on the pullulan derivative backbone	597:655	The active amino groups on the pullulan derivative backbone induced antimicrobial activity against both E. coli and S. aureus.
32204032	7	10	theme	packaging	812:820	arg1	films					822:826	antibacterial food packaging films	793:826	antibacterial food packaging films in the future	793:840	This study can provide an essential guideline for the preparation of antibacterial food packaging films in the future.
32204032	7	11	from	films	822:826	arg1	future					835:840	future	835:840	future	835:840	This study can provide an essential guideline for the preparation of antibacterial food packaging films in the future.
32204032	4	12	theme	light	389:393	arg1	transmittance					395:407	11% light transmittance	385:407	11% light transmittance in the visible region	385:429	In addition, with 11% light transmittance in the visible region, this film exhibited worthwhile light barrier properties.
32204032	7	13	theme	films	822:826	arg1	preparation					778:788	the preparation	774:788	the preparation of antibacterial food packaging films in the future	774:840	This study can provide an essential guideline for the preparation of antibacterial food packaging films in the future.
32204032	4	14	theme	%	387:387	arg1	transmittance					395:407	11% light transmittance	385:407	11% light transmittance in the visible region	385:429	In addition, with 11% light transmittance in the visible region, this film exhibited worthwhile light barrier properties.
32204032	1	15	dep	preservation	150:161	arg1	the					146:148	the	146:148	the	146:148	The preparation of an antibacterial film is imperative for the preservation and transportation of food.
32204032	7	16	theme	antibacterial	793:805	arg1	films					822:826	antibacterial food packaging films	793:826	antibacterial food packaging films in the future	793:840	This study can provide an essential guideline for the preparation of antibacterial food packaging films in the future.
32204032	4	17	theme	worthwhile	452:461	arg1	properties					477:486	worthwhile light barrier properties	452:486	worthwhile light barrier properties	452:486	In addition, with 11% light transmittance in the visible region, this film exhibited worthwhile light barrier properties.
32204032	1	18	theme	film	123:126	arg1	preparation					91:101	The preparation	87:101	The preparation of an antibacterial film	87:126	The preparation of an antibacterial film is imperative for the preservation and transportation of food.
32204032	1	18	theme	film	123:126	arg1	imperative					131:140	imperative	131:140	imperative	131:140	The preparation of an antibacterial film is imperative for the preservation and transportation of food.
32204032	0	19	theme	derivative	45:54	arg1	films					80:84	pullulan derivative antibacterial composite films	36:84	pullulan derivative antibacterial composite films	36:84	Preparation and characterization of pullulan derivative antibacterial composite films.
32204032	7	20	theme	food	807:810	arg1	films					822:826	antibacterial food packaging films	793:826	antibacterial food packaging films in the future	793:840	This study can provide an essential guideline for the preparation of antibacterial food packaging films in the future.
32204032	2	21	theme	derivative/sodium	235:251	arg1	film					272:275	a pullulan derivative/sodium alginate composite film	224:275	a pullulan derivative/sodium alginate composite film	224:275	In the present work, we prepared a pullulan derivative/sodium alginate composite film.
32204032	0	22	theme	pullulan	36:43	arg1	films					80:84	pullulan derivative antibacterial composite films	36:84	pullulan derivative antibacterial composite films	36:84	Preparation and characterization of pullulan derivative antibacterial composite films.
32204032	3	23	theme	composite	283:291	arg1	film					293:296	This composite film	278:296	This composite film	278:296	This composite film exhibited an excellent film-forming ability and water vapor barrier.
32204032	4	24	theme	barrier	469:475	arg1	properties					477:486	worthwhile light barrier properties	452:486	worthwhile light barrier properties	452:486	In addition, with 11% light transmittance in the visible region, this film exhibited worthwhile light barrier properties.
32204032	2	25	theme	pullulan	226:233	arg1	film					272:275	a pullulan derivative/sodium alginate composite film	224:275	a pullulan derivative/sodium alginate composite film	224:275	In the present work, we prepared a pullulan derivative/sodium alginate composite film.
32204032	0	26	theme	composite	70:78	arg1	films					80:84	pullulan derivative antibacterial composite films	36:84	pullulan derivative antibacterial composite films	36:84	Preparation and characterization of pullulan derivative antibacterial composite films.
32204032	3	27	theme	film-forming	321:332	arg1	ability					334:340	an excellent film-forming ability	308:340	an excellent film-forming ability	308:340	This composite film exhibited an excellent film-forming ability and water vapor barrier.
32204032	4	28	theme	light	463:467	arg1	properties					477:486	worthwhile light barrier properties	452:486	worthwhile light barrier properties	452:486	In addition, with 11% light transmittance in the visible region, this film exhibited worthwhile light barrier properties.
32204032	7	29	from	future	835:840	arg1	preparation					778:788	the preparation	774:788	the preparation of antibacterial food packaging films in the future	774:840	This study can provide an essential guideline for the preparation of antibacterial food packaging films in the future.
32204032	0	30	theme	antibacterial	56:68	arg1	films					80:84	pullulan derivative antibacterial composite films	36:84	pullulan derivative antibacterial composite films	36:84	Preparation and characterization of pullulan derivative antibacterial composite films.
32204032	6	31	theme	antimicrobial	665:677	arg1	activity					679:686	antimicrobial activity	665:686	antimicrobial activity against both E. coli and S. aureus	665:721	The active amino groups on the pullulan derivative backbone induced antimicrobial activity against both E. coli and S. aureus.
32204032	5	32	theme	film	582:585	arg1	properties					554:563	the mechanical properties	539:563	the mechanical properties of the composite film (34 MPa)	539:594	Further, the addition of sodium alginate improved the mechanical properties of the composite film (34 MPa).
32204032	5	33	theme	composite	572:580	arg1	34 MPa					588:593	34 MPa	588:593	34 MPa	588:593	Further, the addition of sodium alginate improved the mechanical properties of the composite film (34 MPa).
32204032	5	33	theme	composite	572:580	arg1	film					582:585	the composite film	568:585	the composite film (34 MPa)	568:594	Further, the addition of sodium alginate improved the mechanical properties of the composite film (34 MPa).
32204032	1	34	theme	antibacterial	109:121	arg1	film					123:126	an antibacterial film	106:126	an antibacterial film	106:126	The preparation of an antibacterial film is imperative for the preservation and transportation of food.
32204032	7	35	theme	essential	750:758	arg1	guideline					760:768	an essential guideline	747:768	an essential guideline for the preparation of antibacterial food packaging films in the future	747:840	This study can provide an essential guideline for the preparation of antibacterial food packaging films in the future.
32204032	3	36	theme	excellent	311:319	arg1	ability					334:340	an excellent film-forming ability	308:340	an excellent film-forming ability	308:340	This composite film exhibited an excellent film-forming ability and water vapor barrier.
32204032	6	37	from	groups	614:619	arg1	backbone					648:655	the pullulan derivative backbone	624:655	the pullulan derivative backbone	624:655	The active amino groups on the pullulan derivative backbone induced antimicrobial activity against both E. coli and S. aureus.
32204032	5	38	theme	alginate	521:528	arg1	addition					502:509	the addition	498:509	the addition of sodium alginate	498:528	Further, the addition of sodium alginate improved the mechanical properties of the composite film (34 MPa).
32204032	3	39	theme	vapor	352:356	arg1	barrier					358:364	water vapor barrier	346:364	water vapor barrier	346:364	This composite film exhibited an excellent film-forming ability and water vapor barrier.
32204032	2	40	theme	present	198:204	arg1	work					206:209	the present work	194:209	the present work	194:209	In the present work, we prepared a pullulan derivative/sodium alginate composite film.
32204032	2	41	theme	composite	262:270	arg1	film					272:275	a pullulan derivative/sodium alginate composite film	224:275	a pullulan derivative/sodium alginate composite film	224:275	In the present work, we prepared a pullulan derivative/sodium alginate composite film.
32204032	6	42	theme	derivative	637:646	arg1	backbone					648:655	the pullulan derivative backbone	624:655	the pullulan derivative backbone	624:655	The active amino groups on the pullulan derivative backbone induced antimicrobial activity against both E. coli and S. aureus.
32204032	0	43	theme	films	80:84	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of pullulan derivative antibacterial composite films.
32204032	0	43	theme	films	80:84	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of pullulan derivative antibacterial composite films.
32204032	2	44	theme	alginate	253:260	arg1	film					272:275	a pullulan derivative/sodium alginate composite film	224:275	a pullulan derivative/sodium alginate composite film	224:275	In the present work, we prepared a pullulan derivative/sodium alginate composite film.
32204032	6	45	theme	pullulan	628:635	arg1	backbone					648:655	the pullulan derivative backbone	624:655	the pullulan derivative backbone	624:655	The active amino groups on the pullulan derivative backbone induced antimicrobial activity against both E. coli and S. aureus.
34973766	7	0	theme	anti-angiogenesis	1078:1094	arg1	experiments					1096:1106	anti-angiogenesis experiments	1078:1106	anti-angiogenesis experiments	1078:1106	Furthermore, zebrafish xenografts and anti-angiogenesis experiments indicated that selenization could improve the antitumor activity by inhibiting tumor cell proliferation and migration and blocking angiogenesis.
34973766	2	1	attach	isolated	348:355	arg1	quinata					369:375	Akebia quinata	362:375	Akebia quinata	362:375	Here, an exopoly heteropolysaccharide (AQP70-2B) was firstly isolated from Akebia quinata.
34973766	2	1	attach	isolated	348:355	arg2	AQP70-2B					326:333	AQP70-2B	326:333	AQP70-2B	326:333	Here, an exopoly heteropolysaccharide (AQP70-2B) was firstly isolated from Akebia quinata.
34973766	2	1	attach	isolated	348:355	arg2	heteropolysaccharide					304:323	an exopoly heteropolysaccharide	293:323	an exopoly heteropolysaccharide (AQP70-2B)	293:334	Here, an exopoly heteropolysaccharide (AQP70-2B) was firstly isolated from Akebia quinata.
34973766	5	2	theme	anti-tumor	722:731	arg1	activity					733:740	anti-tumor activity	722:740	anti-tumor activity	722:740	Based on the close relationship between selenium and anti-tumor activity, AQP70-2B was modified with selenium to obtain selenized polysaccharide Se-AQP70-2B.
34973766	0	3	theme	quinata	106:112	arg1	fruits					89:94	the fruits	85:94	the fruits of Akebia quinata	85:112	Preparation and structural properties of selenium modified heteropolysaccharide from the fruits of Akebia quinata and in vitro and in vivo antitumor activity.
34973766	7	4	dep	cell	1193:1196	arg1	proliferation					1198:1210	proliferation	1198:1210	proliferation	1198:1210	Furthermore, zebrafish xenografts and anti-angiogenesis experiments indicated that selenization could improve the antitumor activity by inhibiting tumor cell proliferation and migration and blocking angiogenesis.
34973766	7	4	dep	cell	1193:1196	arg1	migration					1216:1224	migration	1216:1224	migration	1216:1224	Furthermore, zebrafish xenografts and anti-angiogenesis experiments indicated that selenization could improve the antitumor activity by inhibiting tumor cell proliferation and migration and blocking angiogenesis.
34973766	0	5	dep	in	118:119	arg1	vitro					121:125	vitro	121:125	vitro	121:125	Preparation and structural properties of selenium modified heteropolysaccharide from the fruits of Akebia quinata and in vitro and in vivo antitumor activity.
34973766	1	6	theme	most	242:245	arg1	approaches					257:266	the most promising approaches	238:266	the most promising approaches in cancer therapy	238:284	Cancer is a complex disease, and blocking tumor angiogenesis has become one of the most promising approaches in cancer therapy.
34973766	0	7	theme	Akebia	99:104	arg1	quinata					106:112	Akebia quinata	99:112	Akebia quinata	99:112	Preparation and structural properties of selenium modified heteropolysaccharide from the fruits of Akebia quinata and in vitro and in vivo antitumor activity.
34973766	5	8	theme	polysaccharide	799:812	arg1	Se-AQP70-2B					814:824	selenized polysaccharide Se-AQP70-2B	789:824	selenized polysaccharide Se-AQP70-2B	789:824	Based on the close relationship between selenium and anti-tumor activity, AQP70-2B was modified with selenium to obtain selenized polysaccharide Se-AQP70-2B.
34973766	1	9	theme	promising	247:255	arg1	approaches					257:266	the most promising approaches	238:266	the most promising approaches in cancer therapy	238:284	Cancer is a complex disease, and blocking tumor angiogenesis has become one of the most promising approaches in cancer therapy.
34973766	0	10	theme	in	118:119	arg1	activity					149:156	in vitro and in vivo antitumor activity	118:156	in vitro and in vivo antitumor activity	118:156	Preparation and structural properties of selenium modified heteropolysaccharide from the fruits of Akebia quinata and in vitro and in vivo antitumor activity.
34973766	6	11	theme	energy	941:946	arg1	SEM-EDS					973:979	SEM-EDS	973:979	SEM-EDS	973:979	Then, a series of methods for analysis and characterization, especially scanning electron microscopy coupled with energy dispersive spectrometry (SEM-EDS), indicated that Se-AQP70-2B was successfully synthesized.
34973766	6	11	theme	energy	941:946	arg1	spectrometry					959:970	energy dispersive spectrometry	941:970	energy dispersive spectrometry (SEM-EDS)	941:980	Then, a series of methods for analysis and characterization, especially scanning electron microscopy coupled with energy dispersive spectrometry (SEM-EDS), indicated that Se-AQP70-2B was successfully synthesized.
34973766	2	12	theme	Akebia	362:367	arg1	quinata					369:375	Akebia quinata	362:375	Akebia quinata	362:375	Here, an exopoly heteropolysaccharide (AQP70-2B) was firstly isolated from Akebia quinata.
34973766	4	13	theme	-l-Araf-	547:554	arg1	1→					640:641	→1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues	532:666	1→	640:641	The backbone of AQP70-2B consisted of →1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues.
34973766	4	14	dep	1→	640:641	arg1	→3					544:545	→1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues	532:666	→3	544:545	The backbone of AQP70-2B consisted of →1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues.
34973766	1	15	from	approaches	257:266	arg1	therapy					278:284	cancer therapy	271:284	cancer therapy	271:284	Cancer is a complex disease, and blocking tumor angiogenesis has become one of the most promising approaches in cancer therapy.
34973766	1	16	theme	complex	171:177	arg1	disease					179:185	a complex disease	169:185	a complex disease	169:185	Cancer is a complex disease, and blocking tumor angiogenesis has become one of the most promising approaches in cancer therapy.
34973766	1	16	theme	complex	171:177	arg1	Cancer					159:164	Cancer	159:164	Cancer	159:164	Cancer is a complex disease, and blocking tumor angiogenesis has become one of the most promising approaches in cancer therapy.
34973766	1	17	theme	approaches	257:266	arg1	approaches					257:266	the most promising approaches	238:266	the most promising approaches in cancer therapy	238:284	Cancer is a complex disease, and blocking tumor angiogenesis has become one of the most promising approaches in cancer therapy.
34973766	1	17	theme	approaches	257:266	arg1	one					231:233	one	231:233	one	231:233	Cancer is a complex disease, and blocking tumor angiogenesis has become one of the most promising approaches in cancer therapy.
34973766	7	18	theme	tumor	1187:1191	arg1	cell					1193:1196	tumor cell proliferation and migration	1187:1224	tumor cell proliferation and migration	1187:1224	Furthermore, zebrafish xenografts and anti-angiogenesis experiments indicated that selenization could improve the antitumor activity by inhibiting tumor cell proliferation and migration and blocking angiogenesis.
34973766	4	19	theme	-l-Araf-	581:588	arg1	1→					640:641	→1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues	532:666	1→	640:641	The backbone of AQP70-2B consisted of →1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues.
34973766	5	20	theme	selenized	789:797	arg1	Se-AQP70-2B					814:824	selenized polysaccharide Se-AQP70-2B	789:824	selenized polysaccharide Se-AQP70-2B	789:824	Based on the close relationship between selenium and anti-tumor activity, AQP70-2B was modified with selenium to obtain selenized polysaccharide Se-AQP70-2B.
34973766	0	21	theme	structural	16:25	arg1	properties					27:36	structural properties	16:36	structural properties	16:36	Preparation and structural properties of selenium modified heteropolysaccharide from the fruits of Akebia quinata and in vitro and in vivo antitumor activity.
34973766	1	22	theme	cancer	271:276	arg1	therapy					278:284	cancer therapy	271:284	cancer therapy	271:284	Cancer is a complex disease, and blocking tumor angiogenesis has become one of the most promising approaches in cancer therapy.
34973766	4	23	theme	-d-Glcp-	615:622	arg1	1→					640:641	→1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues	532:666	1→	640:641	The backbone of AQP70-2B consisted of →1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues.
34973766	4	24	theme	-l-Araf-	563:570	arg1	1→					640:641	→1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues	532:666	1→	640:641	The backbone of AQP70-2B consisted of →1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues.
34973766	0	25	theme	selenium	41:48	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and structural properties of selenium modified heteropolysaccharide from the fruits of Akebia quinata and in vitro and in vivo antitumor activity.
34973766	0	25	theme	selenium	41:48	arg1	properties					27:36	structural properties	16:36	structural properties	16:36	Preparation and structural properties of selenium modified heteropolysaccharide from the fruits of Akebia quinata and in vitro and in vivo antitumor activity.
34973766	4	26	theme	-l-Araf	535:541	arg1	1→					640:641	→1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues	532:666	1→	640:641	The backbone of AQP70-2B consisted of →1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues.
34973766	0	27	from	fruits	89:94	arg1	activity					149:156	in vitro and in vivo antitumor activity	118:156	in vitro and in vivo antitumor activity	118:156	Preparation and structural properties of selenium modified heteropolysaccharide from the fruits of Akebia quinata and in vitro and in vivo antitumor activity.
34973766	0	27	from	fruits	89:94	arg1	heteropolysaccharide					59:78	heteropolysaccharide	59:78	heteropolysaccharide from the fruits of Akebia quinata	59:112	Preparation and structural properties of selenium modified heteropolysaccharide from the fruits of Akebia quinata and in vitro and in vivo antitumor activity.
34973766	4	28	theme	→1	648:649	arg1	residues					659:666	→1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues	532:666	residues	659:666	The backbone of AQP70-2B consisted of →1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues.
34973766	6	29	theme	scanning	899:906	arg1	methods					845:851	methods	845:851	methods for analysis and characterization	845:885	Then, a series of methods for analysis and characterization, especially scanning electron microscopy coupled with energy dispersive spectrometry (SEM-EDS), indicated that Se-AQP70-2B was successfully synthesized.
34973766	6	29	theme	scanning	899:906	arg1	microscopy					917:926	scanning electron microscopy	899:926	scanning electron microscopy coupled with energy dispersive spectrometry (SEM-EDS)	899:980	Then, a series of methods for analysis and characterization, especially scanning electron microscopy coupled with energy dispersive spectrometry (SEM-EDS), indicated that Se-AQP70-2B was successfully synthesized.
34973766	1	30	theme	blocking	192:199	arg1	angiogenesis					207:218	blocking tumor angiogenesis	192:218	blocking tumor angiogenesis	192:218	Cancer is a complex disease, and blocking tumor angiogenesis has become one of the most promising approaches in cancer therapy.
34973766	6	31	theme	electron	908:915	arg1	methods					845:851	methods	845:851	methods for analysis and characterization	845:885	Then, a series of methods for analysis and characterization, especially scanning electron microscopy coupled with energy dispersive spectrometry (SEM-EDS), indicated that Se-AQP70-2B was successfully synthesized.
34973766	6	31	theme	electron	908:915	arg1	microscopy					917:926	scanning electron microscopy	899:926	scanning electron microscopy coupled with energy dispersive spectrometry (SEM-EDS)	899:980	Then, a series of methods for analysis and characterization, especially scanning electron microscopy coupled with energy dispersive spectrometry (SEM-EDS), indicated that Se-AQP70-2B was successfully synthesized.
34973766	4	32	theme	AQP70-2B	510:517	arg1	backbone					498:505	The backbone	494:505	The backbone of AQP70-2B	494:517	The backbone of AQP70-2B consisted of →1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues.
34973766	0	33	theme	in	131:132	arg1	activity					149:156	in vitro and in vivo antitumor activity	118:156	in vitro and in vivo antitumor activity	118:156	Preparation and structural properties of selenium modified heteropolysaccharide from the fruits of Akebia quinata and in vitro and in vivo antitumor activity.
34973766	7	34	theme	zebrafish	1053:1061	arg1	xenografts					1063:1072	zebrafish xenografts	1053:1072	zebrafish xenografts	1053:1072	Furthermore, zebrafish xenografts and anti-angiogenesis experiments indicated that selenization could improve the antitumor activity by inhibiting tumor cell proliferation and migration and blocking angiogenesis.
34973766	1	35	theme	tumor	201:205	arg1	angiogenesis					207:218	blocking tumor angiogenesis	192:218	blocking tumor angiogenesis	192:218	Cancer is a complex disease, and blocking tumor angiogenesis has become one of the most promising approaches in cancer therapy.
34973766	3	36	theme	Monosaccharide	378:391	arg1	composition					393:403	Monosaccharide composition	378:403	Monosaccharide composition	378:403	Monosaccharide composition indicated that the AQP70-2B was composed of rhamnose, glucose, galactose, and arabinose.
34973766	4	37	theme	-d-Rhap	651:657	arg1	residues					659:666	→1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues	532:666	residues	659:666	The backbone of AQP70-2B consisted of →1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues.
34973766	5	38	mod	modified	756:763	arg1	AQP70-2B					743:750	AQP70-2B	743:750	AQP70-2B	743:750	Based on the close relationship between selenium and anti-tumor activity, AQP70-2B was modified with selenium to obtain selenized polysaccharide Se-AQP70-2B.
34973766	5	38	mod	modified	756:763	arg3	selenium					770:777	selenium	770:777	selenium	770:777	Based on the close relationship between selenium and anti-tumor activity, AQP70-2B was modified with selenium to obtain selenized polysaccharide Se-AQP70-2B.
34973766	0	39	theme	antitumor	139:147	arg1	activity					149:156	in vitro and in vivo antitumor activity	118:156	in vitro and in vivo antitumor activity	118:156	Preparation and structural properties of selenium modified heteropolysaccharide from the fruits of Akebia quinata and in vitro and in vivo antitumor activity.
34973766	4	40	theme	-l-Araf-	599:606	arg1	1→					640:641	→1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues	532:666	1→	640:641	The backbone of AQP70-2B consisted of →1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues.
34973766	7	41	theme	antitumor	1154:1162	arg1	activity					1164:1171	the antitumor activity	1150:1171	the antitumor activity	1150:1171	Furthermore, zebrafish xenografts and anti-angiogenesis experiments indicated that selenization could improve the antitumor activity by inhibiting tumor cell proliferation and migration and blocking angiogenesis.
34973766	4	42	theme	-d-Galp-	631:638	arg1	1→					640:641	→1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues	532:666	1→	640:641	The backbone of AQP70-2B consisted of →1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues.
34973766	2	43	theme	exopoly	296:302	arg1	AQP70-2B					326:333	AQP70-2B	326:333	AQP70-2B	326:333	Here, an exopoly heteropolysaccharide (AQP70-2B) was firstly isolated from Akebia quinata.
34973766	2	43	theme	exopoly	296:302	arg1	heteropolysaccharide					304:323	an exopoly heteropolysaccharide	293:323	an exopoly heteropolysaccharide (AQP70-2B)	293:334	Here, an exopoly heteropolysaccharide (AQP70-2B) was firstly isolated from Akebia quinata.
34973766	6	44	theme	dispersive	948:957	arg1	SEM-EDS					973:979	SEM-EDS	973:979	SEM-EDS	973:979	Then, a series of methods for analysis and characterization, especially scanning electron microscopy coupled with energy dispersive spectrometry (SEM-EDS), indicated that Se-AQP70-2B was successfully synthesized.
34973766	6	44	theme	dispersive	948:957	arg1	spectrometry					959:970	energy dispersive spectrometry	941:970	energy dispersive spectrometry (SEM-EDS)	941:980	Then, a series of methods for analysis and characterization, especially scanning electron microscopy coupled with energy dispersive spectrometry (SEM-EDS), indicated that Se-AQP70-2B was successfully synthesized.
34973766	5	45	theme	close	682:686	arg1	relationship					688:699	the close relationship	678:699	the close relationship between selenium and anti-tumor activity	678:740	Based on the close relationship between selenium and anti-tumor activity, AQP70-2B was modified with selenium to obtain selenized polysaccharide Se-AQP70-2B.
34973766	6	46	theme	methods	845:851	arg1	series					835:840	a series	833:840	a series of methods for analysis and characterization, especially scanning electron microscopy coupled with energy dispersive spectrometry (SEM-EDS),	833:981	Then, a series of methods for analysis and characterization, especially scanning electron microscopy coupled with energy dispersive spectrometry (SEM-EDS), indicated that Se-AQP70-2B was successfully synthesized.
34973766	0	47	dep	in	131:132	arg1	vivo					134:137	vivo	134:137	vivo	134:137	Preparation and structural properties of selenium modified heteropolysaccharide from the fruits of Akebia quinata and in vitro and in vivo antitumor activity.
34952097	2	0	theme	nuclear	374:380	arg1	resonance					391:399	nuclear magnetic resonance	374:399	nuclear magnetic resonance spectra	374:407	The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra demonstrated that W. cibaria NC516.11 had α-(1 → 6) (93.46%) d-glucose linkages with a few α-(1 → 3) (6.54%) d-glucose linked branches.
34952097	0	1	from	characterization	11:26	arg1	dough					137:141	gluten-free dough	125:141	gluten-free dough	125:141	Structural characterization of exopolysaccharides from Weissella cibaria NC516.11 in distiller grains and its improvement in gluten-free dough.
34952097	0	1	from	characterization	11:26	arg1	cibaria					65:71	cibaria	65:71	cibaria	65:71	Structural characterization of exopolysaccharides from Weissella cibaria NC516.11 in distiller grains and its improvement in gluten-free dough.
34952097	0	2	from	improvement	110:120	arg1	dough					137:141	gluten-free dough	125:141	gluten-free dough	125:141	Structural characterization of exopolysaccharides from Weissella cibaria NC516.11 in distiller grains and its improvement in gluten-free dough.
34952097	0	2	from	improvement	110:120	arg1	cibaria					65:71	cibaria	65:71	cibaria	65:71	Structural characterization of exopolysaccharides from Weissella cibaria NC516.11 in distiller grains and its improvement in gluten-free dough.
34952097	6	3	theme	Dynamic	968:974	arg1	rheology					976:983	Dynamic rheology	968:983	Dynamic rheology	968:983	Dynamic rheology indicated that the aqueous solution of EPS is a pseudoplastic fluid, and the higher the concentration of EPS, the greater the viscosity.
34952097	1	4	theme	Chinese	263:269	arg1	Baijiu					271:276	Chinese Baijiu	263:276	Chinese Baijiu	263:276	In this study, an exopolysaccharide (EPS) was produced by Weissella cibaria NC516.11 isolated from distiller grains of Chinese Baijiu.
34952097	7	5	theme	gluten-free	1149:1159	arg1	dough					1161:1165	the gluten-free dough	1145:1165	the gluten-free dough	1145:1165	The addition of EPS to the gluten-free dough showed G' > G″, which could increase the viscoelastic properties of the dough and enhance the gluten network.
34952097	4	6	from	magnification	831:843	arg1	surface					815:821	the surface	811:821	the surface at high magnification	811:843	Scanning electron microscopy showed that the microstructure of EPS had a three-dimensional structure at low magnification and a particle structure that protruded from the surface at high magnification.
34952097	5	7	theme	water-holding	944:956	arg1	capacity					958:965	the water-holding capacity	940:965	the water-holding capacity	940:965	The addition of EPS into dough can promote the cross-linking of starch molecules and increase the water-holding capacity.
34952097	2	8	theme	structural	283:292	arg1	characterization					294:309	The structural characterization	279:309	The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra	279:407	The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra demonstrated that W. cibaria NC516.11 had α-(1 → 6) (93.46%) d-glucose linkages with a few α-(1 → 3) (6.54%) d-glucose linked branches.
34952097	1	9	theme	Baijiu	271:276	arg1	grains					253:258	distiller grains	243:258	distiller grains of Chinese Baijiu	243:276	In this study, an exopolysaccharide (EPS) was produced by Weissella cibaria NC516.11 isolated from distiller grains of Chinese Baijiu.
34952097	0	10	theme	gluten-free	125:135	arg1	dough					137:141	gluten-free dough	125:141	gluten-free dough	125:141	Structural characterization of exopolysaccharides from Weissella cibaria NC516.11 in distiller grains and its improvement in gluten-free dough.
34952097	0	11	from	cibaria	65:71	arg1	improvement					110:120	its improvement	106:120	its improvement in gluten-free dough	106:141	Structural characterization of exopolysaccharides from Weissella cibaria NC516.11 in distiller grains and its improvement in gluten-free dough.
34952097	0	11	from	cibaria	65:71	arg1	exopolysaccharides					31:48	exopolysaccharides	31:48	exopolysaccharides from Weissella cibaria NC516.11 in distiller grains	31:100	Structural characterization of exopolysaccharides from Weissella cibaria NC516.11 in distiller grains and its improvement in gluten-free dough.
34952097	0	11	from	cibaria	65:71	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of exopolysaccharides from Weissella cibaria NC516.11 in distiller grains	0:100	Structural characterization of exopolysaccharides from Weissella cibaria NC516.11 in distiller grains and its improvement in gluten-free dough.
34952097	2	12	theme	α-	500:501	arg1	branches					535:542	a few α-(1 → 3) (6.54%) d-glucose linked branches	494:542	a few α-(1 → 3) (6.54%) d-glucose linked branches	494:542	The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra demonstrated that W. cibaria NC516.11 had α-(1 → 6) (93.46%) d-glucose linkages with a few α-(1 → 3) (6.54%) d-glucose linked branches.
34952097	4	13	theme	low	748:750	arg1	magnification					752:764	low magnification	748:764	low magnification	748:764	Scanning electron microscopy showed that the microstructure of EPS had a three-dimensional structure at low magnification and a particle structure that protruded from the surface at high magnification.
34952097	7	14	theme	EPS	1138:1140	arg1	addition					1126:1133	The addition	1122:1133	The addition of EPS to the gluten-free dough	1122:1165	The addition of EPS to the gluten-free dough showed G' > G″, which could increase the viscoelastic properties of the dough and enhance the gluten network.
34952097	0	15	theme	distiller	85:93	arg1	grains					95:100	distiller grains	85:100	distiller grains	85:100	Structural characterization of exopolysaccharides from Weissella cibaria NC516.11 in distiller grains and its improvement in gluten-free dough.
34952097	5	16	theme	starch	910:915	arg1	molecules					917:925	starch molecules	910:925	starch molecules	910:925	The addition of EPS into dough can promote the cross-linking of starch molecules and increase the water-holding capacity.
34952097	7	17	theme	gluten	1261:1266	arg1	network					1268:1274	the gluten network	1257:1274	the gluten network	1257:1274	The addition of EPS to the gluten-free dough showed G' > G″, which could increase the viscoelastic properties of the dough and enhance the gluten network.
34952097	0	18	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of exopolysaccharides from Weissella cibaria NC516.11 in distiller grains	0:100	Structural characterization of exopolysaccharides from Weissella cibaria NC516.11 in distiller grains and its improvement in gluten-free dough.
34952097	4	19	theme	high	826:829	arg1	magnification					831:843	high magnification	826:843	high magnification	826:843	Scanning electron microscopy showed that the microstructure of EPS had a three-dimensional structure at low magnification and a particle structure that protruded from the surface at high magnification.
34952097	2	20	dep	fourier	335:341	arg1	transform					343:351	transform	343:351	transform infrared spectra and nuclear magnetic resonance spectra	343:407	The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra demonstrated that W. cibaria NC516.11 had α-(1 → 6) (93.46%) d-glucose linkages with a few α-(1 → 3) (6.54%) d-glucose linked branches.
34952097	2	21	link	linked	528:533	arg1	branches					535:542	a few α-(1 → 3) (6.54%) d-glucose linked branches	494:542	a few α-(1 → 3) (6.54%) d-glucose linked branches	494:542	The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra demonstrated that W. cibaria NC516.11 had α-(1 → 6) (93.46%) d-glucose linkages with a few α-(1 → 3) (6.54%) d-glucose linked branches.
34952097	0	22	theme	exopolysaccharides	31:48	arg1	improvement					110:120	its improvement	106:120	its improvement in gluten-free dough	106:141	Structural characterization of exopolysaccharides from Weissella cibaria NC516.11 in distiller grains and its improvement in gluten-free dough.
34952097	0	22	theme	exopolysaccharides	31:48	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of exopolysaccharides from Weissella cibaria NC516.11 in distiller grains	0:100	Structural characterization of exopolysaccharides from Weissella cibaria NC516.11 in distiller grains and its improvement in gluten-free dough.
34952097	2	23	theme	EPS	314:316	arg1	characterization					294:309	The structural characterization	279:309	The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra	279:407	The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra demonstrated that W. cibaria NC516.11 had α-(1 → 6) (93.46%) d-glucose linkages with a few α-(1 → 3) (6.54%) d-glucose linked branches.
34952097	2	24	theme	α-	451:452	arg1	linkages					480:487	α-(1 → 6) (93.46%) d-glucose linkages	451:487	α-(1 → 6) (93.46%) d-glucose linkages	451:487	The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra demonstrated that W. cibaria NC516.11 had α-(1 → 6) (93.46%) d-glucose linkages with a few α-(1 → 3) (6.54%) d-glucose linked branches.
34952097	4	25	theme	EPS	707:709	arg1	microstructure					689:702	the microstructure	685:702	the microstructure of EPS	685:709	Scanning electron microscopy showed that the microstructure of EPS had a three-dimensional structure at low magnification and a particle structure that protruded from the surface at high magnification.
34952097	5	26	theme	molecules	917:925	arg1	cross-linking					893:905	the cross-linking	889:905	the cross-linking of starch molecules	889:925	The addition of EPS into dough can promote the cross-linking of starch molecules and increase the water-holding capacity.
34952097	4	27	theme	electron	653:660	arg1	microscopy					662:671	Scanning electron microscopy	644:671	Scanning electron microscopy	644:671	Scanning electron microscopy showed that the microstructure of EPS had a three-dimensional structure at low magnification and a particle structure that protruded from the surface at high magnification.
34952097	2	28	theme	d-glucose	470:478	arg1	linkages					480:487	α-(1 → 6) (93.46%) d-glucose linkages	451:487	α-(1 → 6) (93.46%) d-glucose linkages	451:487	The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra demonstrated that W. cibaria NC516.11 had α-(1 → 6) (93.46%) d-glucose linkages with a few α-(1 → 3) (6.54%) d-glucose linked branches.
34952097	4	29	contain	had	711:713	arg1	microstructure					689:702	the microstructure	685:702	the microstructure of EPS	685:709	Scanning electron microscopy showed that the microstructure of EPS had a three-dimensional structure at low magnification and a particle structure that protruded from the surface at high magnification.
34952097	4	29	contain	had	711:713	arg2	structure					735:743	a three-dimensional structure	715:743	a three-dimensional structure	715:743	Scanning electron microscopy showed that the microstructure of EPS had a three-dimensional structure at low magnification and a particle structure that protruded from the surface at high magnification.
34952097	3	30	theme	molecular	608:616	arg1	weight					618:623	its molecular weight	604:623	its molecular weight	604:623	The monosaccharide composition of the EPS was glucose, and its molecular weight was 2.82 × 106 Da.
34952097	1	31	attach	isolated	229:236	arg2	cibaria					212:218	cibaria	212:218	cibaria	212:218	In this study, an exopolysaccharide (EPS) was produced by Weissella cibaria NC516.11 isolated from distiller grains of Chinese Baijiu.
34952097	1	31	attach	isolated	229:236	arg1	grains					253:258	distiller grains	243:258	distiller grains of Chinese Baijiu	243:276	In this study, an exopolysaccharide (EPS) was produced by Weissella cibaria NC516.11 isolated from distiller grains of Chinese Baijiu.
34952097	7	32	theme	dough	1239:1243	arg1	properties					1221:1230	the viscoelastic properties	1204:1230	the viscoelastic properties of the dough	1204:1243	The addition of EPS to the gluten-free dough showed G' > G″, which could increase the viscoelastic properties of the dough and enhance the gluten network.
34952097	6	33	dep	higher	1062:1067	arg1	concentration					1073:1085	the concentration	1069:1085	the higher the concentration of EPS	1058:1092	Dynamic rheology indicated that the aqueous solution of EPS is a pseudoplastic fluid, and the higher the concentration of EPS, the greater the viscosity.
34952097	6	34	theme	EPS	1024:1026	arg1	solution					1012:1019	the aqueous solution	1000:1019	the aqueous solution of EPS	1000:1026	Dynamic rheology indicated that the aqueous solution of EPS is a pseudoplastic fluid, and the higher the concentration of EPS, the greater the viscosity.
34952097	6	34	theme	EPS	1024:1026	arg1	fluid					1047:1051	a pseudoplastic fluid	1031:1051	a pseudoplastic fluid	1031:1051	Dynamic rheology indicated that the aqueous solution of EPS is a pseudoplastic fluid, and the higher the concentration of EPS, the greater the viscosity.
34952097	5	35	theme	EPS	862:864	arg1	addition					850:857	The addition	846:857	The addition of EPS into dough	846:875	The addition of EPS into dough can promote the cross-linking of starch molecules and increase the water-holding capacity.
34952097	3	36	theme	monosaccharide	549:562	arg1	composition					564:574	The monosaccharide composition	545:574	The monosaccharide composition of the EPS	545:585	The monosaccharide composition of the EPS was glucose, and its molecular weight was 2.82 × 106 Da.
34952097	3	36	theme	monosaccharide	549:562	arg1	glucose					591:597	glucose	591:597	glucose	591:597	The monosaccharide composition of the EPS was glucose, and its molecular weight was 2.82 × 106 Da.
34952097	2	37	theme	infrared	353:360	arg1	spectra					362:368	infrared spectra	353:368	infrared spectra	353:368	The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra demonstrated that W. cibaria NC516.11 had α-(1 → 6) (93.46%) d-glucose linkages with a few α-(1 → 3) (6.54%) d-glucose linked branches.
34952097	4	38	theme	particle	772:779	arg1	structure					781:789	a particle structure	770:789	a particle structure that protruded from the surface at high magnification	770:843	Scanning electron microscopy showed that the microstructure of EPS had a three-dimensional structure at low magnification and a particle structure that protruded from the surface at high magnification.
34952097	2	39	theme	linked	528:533	arg1	branches					535:542	a few α-(1 → 3) (6.54%) d-glucose linked branches	494:542	a few α-(1 → 3) (6.54%) d-glucose linked branches	494:542	The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra demonstrated that W. cibaria NC516.11 had α-(1 → 6) (93.46%) d-glucose linkages with a few α-(1 → 3) (6.54%) d-glucose linked branches.
34952097	7	40	theme	viscoelastic	1208:1219	arg1	properties					1221:1230	the viscoelastic properties	1204:1230	the viscoelastic properties of the dough	1204:1243	The addition of EPS to the gluten-free dough showed G' > G″, which could increase the viscoelastic properties of the dough and enhance the gluten network.
34952097	4	41	theme	three-dimensional	717:733	arg1	structure					735:743	a three-dimensional structure	715:743	a three-dimensional structure	715:743	Scanning electron microscopy showed that the microstructure of EPS had a three-dimensional structure at low magnification and a particle structure that protruded from the surface at high magnification.
34952097	2	42	theme	d-glucose	518:526	arg1	branches					535:542	a few α-(1 → 3) (6.54%) d-glucose linked branches	494:542	a few α-(1 → 3) (6.54%) d-glucose linked branches	494:542	The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra demonstrated that W. cibaria NC516.11 had α-(1 → 6) (93.46%) d-glucose linkages with a few α-(1 → 3) (6.54%) d-glucose linked branches.
34952097	2	43	contain	had	447:449	arg2	linkages					480:487	α-(1 → 6) (93.46%) d-glucose linkages	451:487	α-(1 → 6) (93.46%) d-glucose linkages	451:487	The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra demonstrated that W. cibaria NC516.11 had α-(1 → 6) (93.46%) d-glucose linkages with a few α-(1 → 3) (6.54%) d-glucose linked branches.
34952097	2	43	contain	had	447:449	arg1	cibaria					430:436	cibaria	430:436	cibaria	430:436	The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra demonstrated that W. cibaria NC516.11 had α-(1 → 6) (93.46%) d-glucose linkages with a few α-(1 → 3) (6.54%) d-glucose linked branches.
34952097	4	44	theme	Scanning	644:651	arg1	microscopy					662:671	Scanning electron microscopy	644:671	Scanning electron microscopy	644:671	Scanning electron microscopy showed that the microstructure of EPS had a three-dimensional structure at low magnification and a particle structure that protruded from the surface at high magnification.
34952097	2	45	theme	few	496:498	arg1	%					515:515	6.54%	511:515	6.54%	511:515	The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra demonstrated that W. cibaria NC516.11 had α-(1 → 6) (93.46%) d-glucose linkages with a few α-(1 → 3) (6.54%) d-glucose linked branches.
34952097	2	45	theme	few	496:498	arg1	1 → 3					503:507	1 → 3	503:507	1 → 3	503:507	The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra demonstrated that W. cibaria NC516.11 had α-(1 → 6) (93.46%) d-glucose linkages with a few α-(1 → 3) (6.54%) d-glucose linked branches.
34952097	2	45	theme	few	496:498	arg1	α-					500:501	a few α-	494:501	a few α-(1 → 3) (6.54%) d-glucose linked branches	494:542	The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra demonstrated that W. cibaria NC516.11 had α-(1 → 6) (93.46%) d-glucose linkages with a few α-(1 → 3) (6.54%) d-glucose linked branches.
34952097	6	46	dep	greater	1099:1105	arg1	viscosity					1111:1119	the viscosity	1107:1119	the greater the viscosity	1095:1119	Dynamic rheology indicated that the aqueous solution of EPS is a pseudoplastic fluid, and the higher the concentration of EPS, the greater the viscosity.
34952097	6	47	theme	pseudoplastic	1033:1045	arg1	solution					1012:1019	the aqueous solution	1000:1019	the aqueous solution of EPS	1000:1026	Dynamic rheology indicated that the aqueous solution of EPS is a pseudoplastic fluid, and the higher the concentration of EPS, the greater the viscosity.
34952097	6	47	theme	pseudoplastic	1033:1045	arg1	fluid					1047:1051	a pseudoplastic fluid	1031:1051	a pseudoplastic fluid	1031:1051	Dynamic rheology indicated that the aqueous solution of EPS is a pseudoplastic fluid, and the higher the concentration of EPS, the greater the viscosity.
34952097	2	48	theme	resonance	391:399	arg1	spectra					401:407	nuclear magnetic resonance spectra	374:407	nuclear magnetic resonance spectra	374:407	The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra demonstrated that W. cibaria NC516.11 had α-(1 → 6) (93.46%) d-glucose linkages with a few α-(1 → 3) (6.54%) d-glucose linked branches.
34952097	6	49	theme	aqueous	1004:1010	arg1	solution					1012:1019	the aqueous solution	1000:1019	the aqueous solution of EPS	1000:1026	Dynamic rheology indicated that the aqueous solution of EPS is a pseudoplastic fluid, and the higher the concentration of EPS, the greater the viscosity.
34952097	6	49	theme	aqueous	1004:1010	arg1	fluid					1047:1051	a pseudoplastic fluid	1031:1051	a pseudoplastic fluid	1031:1051	Dynamic rheology indicated that the aqueous solution of EPS is a pseudoplastic fluid, and the higher the concentration of EPS, the greater the viscosity.
34952097	3	50	theme	EPS	583:585	arg1	composition					564:574	The monosaccharide composition	545:574	The monosaccharide composition of the EPS	545:585	The monosaccharide composition of the EPS was glucose, and its molecular weight was 2.82 × 106 Da.
34952097	3	50	theme	EPS	583:585	arg1	glucose					591:597	glucose	591:597	glucose	591:597	The monosaccharide composition of the EPS was glucose, and its molecular weight was 2.82 × 106 Da.
34952097	2	51	theme	magnetic	382:389	arg1	resonance					391:399	nuclear magnetic resonance	374:399	nuclear magnetic resonance spectra	374:407	The structural characterization of EPS determined using fourier transform infrared spectra and nuclear magnetic resonance spectra demonstrated that W. cibaria NC516.11 had α-(1 → 6) (93.46%) d-glucose linkages with a few α-(1 → 3) (6.54%) d-glucose linked branches.
34952097	1	52	theme	distiller	243:251	arg1	grains					253:258	distiller grains	243:258	distiller grains of Chinese Baijiu	243:276	In this study, an exopolysaccharide (EPS) was produced by Weissella cibaria NC516.11 isolated from distiller grains of Chinese Baijiu.
32191427	8	0	theme	acaricide	1050:1058	arg1	Amitraz					1060:1066	the water-insoluble acaricide Amitraz	1030:1066	the water-insoluble acaricide Amitraz	1030:1066	It was selected as the carrier material for the water-insoluble acaricide Amitraz.
32191427	1	1	theme	aquatic	159:165	arg1	environments					167:178	aquatic environments	159:178	aquatic environments	159:178	Safe application of water-insoluble acaricides requires fast release from solid dosage systems into aquatic environments.
32191427	9	2	theme	release	1074:1080	arg1	rates					1082:1086	Fast release rates	1069:1086	Fast release rates	1069:1086	Fast release rates were observed for composites containing 5, 10, and 20% (w/w) of the pesticide.
32191427	10	3	theme	particle	1198:1205	arg1	size					1207:1210	the particle size	1194:1210	the particle size of the released Amitraz powder	1194:1241	A significant reduction in the particle size of the released Amitraz powder was observed, which is ascribed to the high-temperature compounding procedure.
32191427	8	4	theme	water-insoluble	1034:1048	arg1	Amitraz					1060:1066	the water-insoluble acaricide Amitraz	1030:1066	the water-insoluble acaricide Amitraz	1030:1066	It was selected as the carrier material for the water-insoluble acaricide Amitraz.
32191427	9	5	theme	pesticide	1156:1164	arg1	%					1141:1141	5, 10, and 20%	1128:1141	5, 10, and 20% (w/w) of the pesticide	1128:1164	Fast release rates were observed for composites containing 5, 10, and 20% (w/w) of the pesticide.
32191427	9	5	theme	pesticide	1156:1164	arg1	pesticide					1156:1164	the pesticide	1152:1164	the pesticide	1152:1164	Fast release rates were observed for composites containing 5, 10, and 20% (w/w) of the pesticide.
32191427	9	5	theme	pesticide	1156:1164	arg1	w/w					1144:1146	w/w	1144:1146	w/w	1144:1146	Fast release rates were observed for composites containing 5, 10, and 20% (w/w) of the pesticide.
32191427	10	6	theme	powder	1236:1241	arg1	size					1207:1210	the particle size	1194:1210	the particle size of the released Amitraz powder	1194:1241	A significant reduction in the particle size of the released Amitraz powder was observed, which is ascribed to the high-temperature compounding procedure.
32191427	10	7	theme	Amitraz	1228:1234	arg1	powder					1236:1241	the released Amitraz powder	1215:1241	the released Amitraz powder	1215:1241	A significant reduction in the particle size of the released Amitraz powder was observed, which is ascribed to the high-temperature compounding procedure.
32191427	4	8	theme	layered	530:536	arg1	LDH					556:558	LDH	556:558	LDH	556:558	The nanofillers included a layered double hydroxide (LDH), cellulose nanofibers (CNF), and stearic acid.
32191427	4	8	theme	layered	530:536	arg1	hydroxide					545:553	a layered double hydroxide	528:553	a layered double hydroxide (LDH)	528:559	The nanofillers included a layered double hydroxide (LDH), cellulose nanofibers (CNF), and stearic acid.
32191427	1	9	from	systems	146:152	arg1	release					120:126	fast release	115:126	fast release from solid dosage systems into aquatic environments	115:178	Safe application of water-insoluble acaricides requires fast release from solid dosage systems into aquatic environments.
32191427	1	10	theme	Safe	59:62	arg1	application					64:74	Safe application	59:74	Safe application of water-insoluble acaricides	59:104	Safe application of water-insoluble acaricides requires fast release from solid dosage systems into aquatic environments.
32191427	5	11	theme	compounds	649:657	arg1	retrogradation					627:640	The time-dependent retrogradation	608:640	The time-dependent retrogradation of the compounds	608:657	The time-dependent retrogradation of the compounds was monitored by X-ray diffraction (XRD) and dynamic mechanical thermal analysis (DMA).
32191427	3	12	theme	extrusion-compounding	472:492	arg1	process					494:500	a twin-screw extrusion-compounding process	459:500	a twin-screw extrusion-compounding process	459:500	Several glycerol-plasticized thermoplastic dextrin-based nanocomposites were prepared with a twin-screw extrusion-compounding process.
32191427	7	13	theme	stearic	964:970	arg1	acid					972:975	stearic acid	964:975	stearic acid	964:975	The most promising nanocomposite included both stearic acid and CNF.
32191427	2	14	theme	matrix	266:271	arg1	form					208:211	a water-soluble form	192:211	a water-soluble form	192:211	Dextrin is a water-soluble form of partially hydrolyzed starch, which may be used as matrix material for these systems if retrogradation can be inhibited by the inclusion of nanofillers.
32191427	2	14	theme	matrix	266:271	arg1	material					273:280	matrix material	266:280	matrix material	266:280	Dextrin is a water-soluble form of partially hydrolyzed starch, which may be used as matrix material for these systems if retrogradation can be inhibited by the inclusion of nanofillers.
32191427	2	15	used	used	258:261	arg2	form					208:211	a water-soluble form	192:211	a water-soluble form	192:211	Dextrin is a water-soluble form of partially hydrolyzed starch, which may be used as matrix material for these systems if retrogradation can be inhibited by the inclusion of nanofillers.
32191427	2	15	used	used	258:261	arg2	Dextrin					181:187	Dextrin	181:187	Dextrin	181:187	Dextrin is a water-soluble form of partially hydrolyzed starch, which may be used as matrix material for these systems if retrogradation can be inhibited by the inclusion of nanofillers.
32191427	2	15	used	used	258:261	arg2	material					273:280	matrix material	266:280	matrix material	266:280	Dextrin is a water-soluble form of partially hydrolyzed starch, which may be used as matrix material for these systems if retrogradation can be inhibited by the inclusion of nanofillers.
32191427	3	16	theme	thermoplastic	397:409	arg1	nanocomposites					425:438	Several glycerol-plasticized thermoplastic dextrin-based nanocomposites	368:438	Several glycerol-plasticized thermoplastic dextrin-based nanocomposites	368:438	Several glycerol-plasticized thermoplastic dextrin-based nanocomposites were prepared with a twin-screw extrusion-compounding process.
32191427	4	17	theme	double	538:543	arg1	LDH					556:558	LDH	556:558	LDH	556:558	The nanofillers included a layered double hydroxide (LDH), cellulose nanofibers (CNF), and stearic acid.
32191427	4	17	theme	double	538:543	arg1	hydroxide					545:553	a layered double hydroxide	528:553	a layered double hydroxide (LDH)	528:559	The nanofillers included a layered double hydroxide (LDH), cellulose nanofibers (CNF), and stearic acid.
32191427	8	18	theme	carrier	1009:1015	arg1	material					1017:1024	the carrier material	1005:1024	the carrier material for the water-insoluble acaricide Amitraz	1005:1066	It was selected as the carrier material for the water-insoluble acaricide Amitraz.
32191427	8	18	theme	carrier	1009:1015	arg1	It					986:987	It	986:987	It	986:987	It was selected as the carrier material for the water-insoluble acaricide Amitraz.
32191427	1	19	theme	water-insoluble	79:93	arg1	acaricides					95:104	water-insoluble acaricides	79:104	water-insoluble acaricides	79:104	Safe application of water-insoluble acaricides requires fast release from solid dosage systems into aquatic environments.
32191427	10	20	from	reduction	1181:1189	arg1	size					1207:1210	the particle size	1194:1210	the particle size of the released Amitraz powder	1194:1241	A significant reduction in the particle size of the released Amitraz powder was observed, which is ascribed to the high-temperature compounding procedure.
32191427	3	21	theme	dextrin-based	411:423	arg1	nanocomposites					425:438	Several glycerol-plasticized thermoplastic dextrin-based nanocomposites	368:438	Several glycerol-plasticized thermoplastic dextrin-based nanocomposites	368:438	Several glycerol-plasticized thermoplastic dextrin-based nanocomposites were prepared with a twin-screw extrusion-compounding process.
32191427	5	22	theme	X-ray	676:680	arg1	XRD					695:697	XRD	695:697	XRD	695:697	The time-dependent retrogradation of the compounds was monitored by X-ray diffraction (XRD) and dynamic mechanical thermal analysis (DMA).
32191427	5	22	theme	X-ray	676:680	arg1	diffraction					682:692	X-ray diffraction	676:692	X-ray diffraction (XRD)	676:698	The time-dependent retrogradation of the compounds was monitored by X-ray diffraction (XRD) and dynamic mechanical thermal analysis (DMA).
32191427	1	23	theme	acaricides	95:104	arg1	application					64:74	Safe application	59:74	Safe application of water-insoluble acaricides	59:104	Safe application of water-insoluble acaricides requires fast release from solid dosage systems into aquatic environments.
32191427	10	24	theme	compounding	1299:1309	arg1	procedure					1311:1319	the high-temperature compounding procedure	1278:1319	the high-temperature compounding procedure	1278:1319	A significant reduction in the particle size of the released Amitraz powder was observed, which is ascribed to the high-temperature compounding procedure.
32191427	3	25	theme	twin-screw	461:470	arg1	process					494:500	a twin-screw extrusion-compounding process	459:500	a twin-screw extrusion-compounding process	459:500	Several glycerol-plasticized thermoplastic dextrin-based nanocomposites were prepared with a twin-screw extrusion-compounding process.
32191427	3	26	theme	Several	368:374	arg1	nanocomposites					425:438	Several glycerol-plasticized thermoplastic dextrin-based nanocomposites	368:438	Several glycerol-plasticized thermoplastic dextrin-based nanocomposites	368:438	Several glycerol-plasticized thermoplastic dextrin-based nanocomposites were prepared with a twin-screw extrusion-compounding process.
32191427	5	27	theme	time-dependent	612:625	arg1	retrogradation					627:640	The time-dependent retrogradation	608:640	The time-dependent retrogradation of the compounds	608:657	The time-dependent retrogradation of the compounds was monitored by X-ray diffraction (XRD) and dynamic mechanical thermal analysis (DMA).
32191427	10	28	theme	high-temperature	1282:1297	arg1	procedure					1311:1319	the high-temperature compounding procedure	1278:1319	the high-temperature compounding procedure	1278:1319	A significant reduction in the particle size of the released Amitraz powder was observed, which is ascribed to the high-temperature compounding procedure.
32191427	6	29	from	acid	803:806	arg1	formulation					815:825	the formulation	811:825	the formulation	811:825	XRD showed that composite samples that included stearic acid in the formulation led to the formation of an amylose-lipid complex and a stable crystallinity during aging.
32191427	3	30	theme	glycerol-plasticized	376:395	arg1	nanocomposites					425:438	Several glycerol-plasticized thermoplastic dextrin-based nanocomposites	368:438	Several glycerol-plasticized thermoplastic dextrin-based nanocomposites	368:438	Several glycerol-plasticized thermoplastic dextrin-based nanocomposites were prepared with a twin-screw extrusion-compounding process.
32191427	4	31	theme	stearic	594:600	arg1	acid					602:605	stearic acid	594:605	stearic acid	594:605	The nanofillers included a layered double hydroxide (LDH), cellulose nanofibers (CNF), and stearic acid.
32191427	6	32	theme	stable	882:887	arg1	crystallinity					889:901	a stable crystallinity	880:901	a stable crystallinity during aging	880:914	XRD showed that composite samples that included stearic acid in the formulation led to the formation of an amylose-lipid complex and a stable crystallinity during aging.
32191427	1	33	theme	fast	115:118	arg1	release					120:126	fast release	115:126	fast release from solid dosage systems into aquatic environments	115:178	Safe application of water-insoluble acaricides requires fast release from solid dosage systems into aquatic environments.
32191427	0	34	theme	Solid	39:43	arg1	Forms					52:56	Solid Dosage Forms	39:56	Solid Dosage Forms	39:56	Dextrin Nanocomposites as Matrices for Solid Dosage Forms.
32191427	10	35	theme	released	1219:1226	arg1	powder					1236:1241	the released Amitraz powder	1215:1241	the released Amitraz powder	1215:1241	A significant reduction in the particle size of the released Amitraz powder was observed, which is ascribed to the high-temperature compounding procedure.
32191427	4	36	theme	cellulose	562:570	arg1	CNF					584:586	CNF	584:586	CNF	584:586	The nanofillers included a layered double hydroxide (LDH), cellulose nanofibers (CNF), and stearic acid.
32191427	4	36	theme	cellulose	562:570	arg1	nanofibers					572:581	cellulose nanofibers	562:581	cellulose nanofibers (CNF)	562:587	The nanofillers included a layered double hydroxide (LDH), cellulose nanofibers (CNF), and stearic acid.
32191427	6	37	theme	complex	868:874	arg1	formation					838:846	the formation	834:846	the formation of an amylose-lipid complex	834:874	XRD showed that composite samples that included stearic acid in the formulation led to the formation of an amylose-lipid complex and a stable crystallinity during aging.
32191427	6	37	theme	complex	868:874	arg1	crystallinity					889:901	a stable crystallinity	880:901	a stable crystallinity during aging	880:914	XRD showed that composite samples that included stearic acid in the formulation led to the formation of an amylose-lipid complex and a stable crystallinity during aging.
32191427	2	38	theme	starch	237:242	arg1	form					208:211	a water-soluble form	192:211	a water-soluble form	192:211	Dextrin is a water-soluble form of partially hydrolyzed starch, which may be used as matrix material for these systems if retrogradation can be inhibited by the inclusion of nanofillers.
32191427	2	38	theme	starch	237:242	arg1	material					273:280	matrix material	266:280	matrix material	266:280	Dextrin is a water-soluble form of partially hydrolyzed starch, which may be used as matrix material for these systems if retrogradation can be inhibited by the inclusion of nanofillers.
32191427	2	38	theme	starch	237:242	arg1	Dextrin					181:187	Dextrin	181:187	Dextrin	181:187	Dextrin is a water-soluble form of partially hydrolyzed starch, which may be used as matrix material for these systems if retrogradation can be inhibited by the inclusion of nanofillers.
32191427	0	39	theme	Dosage	45:50	arg1	Forms					52:56	Solid Dosage Forms	39:56	Solid Dosage Forms	39:56	Dextrin Nanocomposites as Matrices for Solid Dosage Forms.
32191427	5	40	theme	dynamic	704:710	arg1	DMA					741:743	DMA	741:743	DMA	741:743	The time-dependent retrogradation of the compounds was monitored by X-ray diffraction (XRD) and dynamic mechanical thermal analysis (DMA).
32191427	5	40	theme	dynamic	704:710	arg1	analysis					731:738	dynamic mechanical thermal analysis	704:738	dynamic mechanical thermal analysis (DMA)	704:744	The time-dependent retrogradation of the compounds was monitored by X-ray diffraction (XRD) and dynamic mechanical thermal analysis (DMA).
32191427	6	41	theme	amylose-lipid	854:866	arg1	complex					868:874	an amylose-lipid complex	851:874	an amylose-lipid complex	851:874	XRD showed that composite samples that included stearic acid in the formulation led to the formation of an amylose-lipid complex and a stable crystallinity during aging.
32191427	2	42	theme	hydrolyzed	226:235	arg1	starch					237:242	partially hydrolyzed starch	216:242	partially hydrolyzed starch	216:242	Dextrin is a water-soluble form of partially hydrolyzed starch, which may be used as matrix material for these systems if retrogradation can be inhibited by the inclusion of nanofillers.
32191427	7	43	theme	promising	926:934	arg1	nanocomposite					936:948	The most promising nanocomposite	917:948	The most promising nanocomposite	917:948	The most promising nanocomposite included both stearic acid and CNF.
32191427	6	44	theme	stearic	795:801	arg1	acid					803:806	stearic acid	795:806	stearic acid in the formulation	795:825	XRD showed that composite samples that included stearic acid in the formulation led to the formation of an amylose-lipid complex and a stable crystallinity during aging.
32191427	5	45	theme	mechanical	712:721	arg1	DMA					741:743	DMA	741:743	DMA	741:743	The time-dependent retrogradation of the compounds was monitored by X-ray diffraction (XRD) and dynamic mechanical thermal analysis (DMA).
32191427	5	45	theme	mechanical	712:721	arg1	analysis					731:738	dynamic mechanical thermal analysis	704:738	dynamic mechanical thermal analysis (DMA)	704:744	The time-dependent retrogradation of the compounds was monitored by X-ray diffraction (XRD) and dynamic mechanical thermal analysis (DMA).
32191427	6	46	theme	composite	763:771	arg1	samples					773:779	composite samples	763:779	composite samples that included stearic acid in the formulation	763:825	XRD showed that composite samples that included stearic acid in the formulation led to the formation of an amylose-lipid complex and a stable crystallinity during aging.
32191427	1	47	theme	solid	133:137	arg1	systems					146:152	solid dosage systems	133:152	solid dosage systems	133:152	Safe application of water-insoluble acaricides requires fast release from solid dosage systems into aquatic environments.
32191427	10	48	theme	significant	1169:1179	arg1	reduction					1181:1189	A significant reduction	1167:1189	A significant reduction in the particle size of the released Amitraz powder	1167:1241	A significant reduction in the particle size of the released Amitraz powder was observed, which is ascribed to the high-temperature compounding procedure.
32191427	5	49	theme	thermal	723:729	arg1	DMA					741:743	DMA	741:743	DMA	741:743	The time-dependent retrogradation of the compounds was monitored by X-ray diffraction (XRD) and dynamic mechanical thermal analysis (DMA).
32191427	5	49	theme	thermal	723:729	arg1	analysis					731:738	dynamic mechanical thermal analysis	704:738	dynamic mechanical thermal analysis (DMA)	704:744	The time-dependent retrogradation of the compounds was monitored by X-ray diffraction (XRD) and dynamic mechanical thermal analysis (DMA).
32191427	1	50	theme	dosage	139:144	arg1	systems					146:152	solid dosage systems	133:152	solid dosage systems	133:152	Safe application of water-insoluble acaricides requires fast release from solid dosage systems into aquatic environments.
32191427	2	51	theme	water-soluble	194:206	arg1	form					208:211	a water-soluble form	192:211	a water-soluble form	192:211	Dextrin is a water-soluble form of partially hydrolyzed starch, which may be used as matrix material for these systems if retrogradation can be inhibited by the inclusion of nanofillers.
32191427	2	51	theme	water-soluble	194:206	arg1	material					273:280	matrix material	266:280	matrix material	266:280	Dextrin is a water-soluble form of partially hydrolyzed starch, which may be used as matrix material for these systems if retrogradation can be inhibited by the inclusion of nanofillers.
32191427	2	51	theme	water-soluble	194:206	arg1	Dextrin					181:187	Dextrin	181:187	Dextrin	181:187	Dextrin is a water-soluble form of partially hydrolyzed starch, which may be used as matrix material for these systems if retrogradation can be inhibited by the inclusion of nanofillers.
32191427	9	52	contain	containing	1117:1126	arg1	composites					1106:1115	composites	1106:1115	composites containing 5, 10, and 20% (w/w) of the pesticide	1106:1164	Fast release rates were observed for composites containing 5, 10, and 20% (w/w) of the pesticide.
32191427	9	52	contain	containing	1117:1126	arg2	pesticide					1156:1164	the pesticide	1152:1164	the pesticide	1152:1164	Fast release rates were observed for composites containing 5, 10, and 20% (w/w) of the pesticide.
32191427	9	52	contain	containing	1117:1126	arg2	w/w					1144:1146	w/w	1144:1146	w/w	1144:1146	Fast release rates were observed for composites containing 5, 10, and 20% (w/w) of the pesticide.
32191427	9	52	contain	containing	1117:1126	arg2	%					1141:1141	5, 10, and 20%	1128:1141	5, 10, and 20% (w/w) of the pesticide	1128:1164	Fast release rates were observed for composites containing 5, 10, and 20% (w/w) of the pesticide.
32191427	2	53	theme	nanofillers	355:365	arg1	inclusion					342:350	the inclusion	338:350	the inclusion of nanofillers	338:365	Dextrin is a water-soluble form of partially hydrolyzed starch, which may be used as matrix material for these systems if retrogradation can be inhibited by the inclusion of nanofillers.
32191427	9	54	theme	Fast	1069:1072	arg1	rates					1082:1086	Fast release rates	1069:1086	Fast release rates	1069:1086	Fast release rates were observed for composites containing 5, 10, and 20% (w/w) of the pesticide.
31879786	15	0	theme	body	2856:2859	arg1	weight					2861:2866	body weight	2856:2866	body weight (by >100%; P < 0.01)	2856:2887	Compared with the YBG group, the YBG-(Abx) group of Expt 5 showed aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04).
31879786	13	1	theme	higher	2529:2534	arg1	frequencies					2536:2546	higher frequencies	2529:2546	higher frequencies of Tregs (by 32%; P = 0.043)	2529:2575	Compared with controls, the YBG group of Expt 2 showed higher frequencies of Tregs (by 32%; P = 0.043) in the gut mucosa.
31879786	0	2	theme	C57BL/6	197:203	arg1	Mice					205:208	C57BL/6 Mice	197:208	C57BL/6 Mice	197:208	Pretreatment with Yeast-Derived Complex Dietary Polysaccharides Suppresses Gut Inflammation, Alters the Microbiota Composition, and Increases Immune Regulatory Short-Chain Fatty Acid Production in C57BL/6 Mice.
31879786	3	3	from	impact	489:494	arg1	susceptibility					583:596	susceptibility	583:596	susceptibility to colitis	583:607	OBJECTIVES The aim of this study was to investigate the impact of orally administering highly purified, yeast-derived BG (YBG; β-1,3/1,6-d-glucan) on susceptibility to colitis.
31879786	8	4	theme	female	1427:1432	arg1	mice					1485:1488	female B6 Foxp3-green fluorescent protein (-GFP) reporter mice	1427:1488	female B6 Foxp3-green fluorescent protein (-GFP) reporter mice	1427:1488	Expt 4: female B6 Foxp3-green fluorescent protein (-GFP) reporter mice were treated as in Expt 3, and intestinal T-regulatory cell (Treg) frequencies and immune phenotypes were determined.
31879786	1	5	theme	dietary	258:264	arg1	CDPs					283:286	CDPs	283:286	CDPs	283:286	BACKGROUND β-Glucans (BGs), a group of complex dietary polysaccharides (CDPs), are available as dietary supplements.
31879786	1	5	theme	dietary	258:264	arg1	polysaccharides					266:280	complex dietary polysaccharides	250:280	complex dietary polysaccharides (CDPs)	250:287	BACKGROUND β-Glucans (BGs), a group of complex dietary polysaccharides (CDPs), are available as dietary supplements.
31879786	12	6	dep	propionic	2404:2412	arg1	%					2420:2420	47%	2418:2420	47%; P = 0.026	2418:2431	The YBG group also showed significantly higher concentrations of fecal SCFAs such as acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids.
31879786	6	7	theme	fecal	1093:1097	arg1	concentrations					1104:1117	fecal SCFA concentrations	1093:1117	fecal SCFA concentrations	1093:1117	Expt 2: female B6 mice were treated with saline or YBG for 30 d and intestinal immune phenotype, gut microbiota composition, and fecal SCFA concentrations were determined.
31879786	8	8	theme	Foxp3-green	1437:1447	arg1	mice					1485:1488	female B6 Foxp3-green fluorescent protein (-GFP) reporter mice	1427:1488	female B6 Foxp3-green fluorescent protein (-GFP) reporter mice	1427:1488	Expt 4: female B6 Foxp3-green fluorescent protein (-GFP) reporter mice were treated as in Expt 3, and intestinal T-regulatory cell (Treg) frequencies and immune phenotypes were determined.
31879786	9	9	theme	female	1616:1621	arg1	mice					1623:1626	female mice	1616:1626	female mice	1616:1626	Expt 5: female mice were treated as in Expt 1, given drinking water with or without antibiotics during days 16-40, and colitis severity and intestinal cytokine production were determined.
31879786	3	10	theme	purified	527:534	arg1	YBG					555:557	YBG	555:557	YBG; β-1,3/1,6-d-glucan	555:577	OBJECTIVES The aim of this study was to investigate the impact of orally administering highly purified, yeast-derived BG (YBG; β-1,3/1,6-d-glucan) on susceptibility to colitis.
31879786	3	10	theme	purified	527:534	arg1	BG					551:552	highly purified, yeast-derived BG	520:552	highly purified, yeast-derived BG (YBG; β-1,3/1,6-d-glucan)	520:578	OBJECTIVES The aim of this study was to investigate the impact of orally administering highly purified, yeast-derived BG (YBG; β-1,3/1,6-d-glucan) on susceptibility to colitis.
31879786	8	11	theme	protein	1461:1467	arg1	mice					1485:1488	female B6 Foxp3-green fluorescent protein (-GFP) reporter mice	1427:1488	female B6 Foxp3-green fluorescent protein (-GFP) reporter mice	1427:1488	Expt 4: female B6 Foxp3-green fluorescent protein (-GFP) reporter mice were treated as in Expt 3, and intestinal T-regulatory cell (Treg) frequencies and immune phenotypes were determined.
31879786	15	12	theme	colonic	2893:2899	arg1	score					2914:2918	colonic inflammation score	2893:2918	colonic inflammation score (by 42%; P = 0.04)	2893:2937	Compared with the YBG group, the YBG-(Abx) group of Expt 5 showed aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04).
31879786	15	13	theme	weight	2861:2866	arg1	loss					2848:2851	loss	2848:2851	loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04)	2848:2937	Compared with the YBG group, the YBG-(Abx) group of Expt 5 showed aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04).
31879786	2	14	from	effects	341:347	arg1	inflammation					399:410	gut inflammation	395:410	gut inflammation	395:410	However, the effects of orally administered highly purified BGs on gut inflammation are largely unknown.
31879786	12	15	dep	%	2420:2420	arg1	P = 0.026					2423:2431	P = 0.026	2423:2431	47%; P = 0.026	2418:2431	The YBG group also showed significantly higher concentrations of fecal SCFAs such as acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids.
31879786	6	16	theme	microbiota	1065:1074	arg1	composition					1076:1086	gut microbiota composition	1061:1086	gut microbiota composition	1061:1086	Expt 2: female B6 mice were treated with saline or YBG for 30 d and intestinal immune phenotype, gut microbiota composition, and fecal SCFA concentrations were determined.
31879786	8	17	theme	-GFP	1470:1473	arg1	mice					1485:1488	female B6 Foxp3-green fluorescent protein (-GFP) reporter mice	1427:1488	female B6 Foxp3-green fluorescent protein (-GFP) reporter mice	1427:1488	Expt 4: female B6 Foxp3-green fluorescent protein (-GFP) reporter mice were treated as in Expt 3, and intestinal T-regulatory cell (Treg) frequencies and immune phenotypes were determined.
31879786	1	18	theme	BACKGROUND	211:220	arg1	group					241:245	a group	239:245	a group of complex dietary polysaccharides (CDPs)	239:287	BACKGROUND β-Glucans (BGs), a group of complex dietary polysaccharides (CDPs), are available as dietary supplements.
31879786	1	18	theme	BACKGROUND	211:220	arg1	polysaccharides					266:280	complex dietary polysaccharides	250:280	complex dietary polysaccharides (CDPs)	250:287	BACKGROUND β-Glucans (BGs), a group of complex dietary polysaccharides (CDPs), are available as dietary supplements.
31879786	1	18	theme	BACKGROUND	211:220	arg1	BGs					233:235	BGs	233:235	BGs	233:235	BACKGROUND β-Glucans (BGs), a group of complex dietary polysaccharides (CDPs), are available as dietary supplements.
31879786	1	18	theme	BACKGROUND	211:220	arg1	β-Glucans					222:230	BACKGROUND β-Glucans	211:230	BACKGROUND β-Glucans (BGs)	211:236	BACKGROUND β-Glucans (BGs), a group of complex dietary polysaccharides (CDPs), are available as dietary supplements.
31879786	7	19	theme	SCFA	1382:1385	arg1	concentrations					1387:1400	fecal SCFA concentrations	1376:1400	fecal SCFA concentrations	1376:1400	Expt 3: female B6 mice were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30, and gut immune phenotype and fecal SCFA concentrations were determined.
31879786	7	20	theme	drinking	1192:1199	arg1	water					1201:1205	given drinking water	1186:1205	given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30	1186:1344	Expt 3: female B6 mice were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30, and gut immune phenotype and fecal SCFA concentrations were determined.
31879786	4	21	theme	C57BL/6	633:639	arg1	mice					646:649	Eight-week-old C57BL/6 (B6) mice	618:649	METHODS Eight-week-old C57BL/6 (B6) mice	610:649	METHODS Eight-week-old C57BL/6 (B6) mice were used in a series of experiments.
31879786	12	22	dep	acetic	2376:2381	arg1	%					2389:2389	37%; P = 0.016	2387:2400	37%; P = 0.016	2387:2400	The YBG group also showed significantly higher concentrations of fecal SCFAs such as acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids.
31879786	8	23	theme	reporter	1476:1483	arg1	mice					1485:1488	female B6 Foxp3-green fluorescent protein (-GFP) reporter mice	1427:1488	female B6 Foxp3-green fluorescent protein (-GFP) reporter mice	1427:1488	Expt 4: female B6 Foxp3-green fluorescent protein (-GFP) reporter mice were treated as in Expt 3, and intestinal T-regulatory cell (Treg) frequencies and immune phenotypes were determined.
31879786	7	24	dep	antibiotics	1223:1233	arg1	[Abx					1235:1238	[Abx	1235:1238	[Abx	1235:1238	Expt 3: female B6 mice were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30, and gut immune phenotype and fecal SCFA concentrations were determined.
31879786	6	25	theme	gut	1061:1063	arg1	composition					1076:1086	gut microbiota composition	1061:1086	gut microbiota composition	1061:1086	Expt 2: female B6 mice were treated with saline or YBG for 30 d and intestinal immune phenotype, gut microbiota composition, and fecal SCFA concentrations were determined.
31879786	13	26	theme	gut	2584:2586	arg1	mucosa					2588:2593	the gut mucosa	2580:2593	the gut mucosa	2580:2593	Compared with controls, the YBG group of Expt 2 showed higher frequencies of Tregs (by 32%; P = 0.043) in the gut mucosa.
31879786	4	27	theme	B6	642:643	arg1	mice					646:649	Eight-week-old C57BL/6 (B6) mice	618:649	METHODS Eight-week-old C57BL/6 (B6) mice	610:649	METHODS Eight-week-old C57BL/6 (B6) mice were used in a series of experiments.
31879786	0	28	theme	Regulatory	149:158	arg1	Acid					178:181	Regulatory Short-Chain Fatty Acid	149:181	Immune Regulatory Short-Chain Fatty Acid Production in C57BL/6 Mice	142:208	Pretreatment with Yeast-Derived Complex Dietary Polysaccharides Suppresses Gut Inflammation, Alters the Microbiota Composition, and Increases Immune Regulatory Short-Chain Fatty Acid Production in C57BL/6 Mice.
31879786	5	29	theme	immune	930:935	arg1	phenotype					937:945	intestinal immune phenotype	919:945	intestinal immune phenotype	919:945	Experiment (Expt) 1: male and female mice were treated every day, for 40 d, with saline (control) or 250 μg YBG, followed by 2.5% (wt:vol) dextran sulfate sodium (DSS) in drinking water during days 30-35; and colitis severity and intestinal immune phenotype were determined.
31879786	14	30	theme	SCFA	2673:2676	arg1	Expt					2694:2697	Expt 3	2694:2699	Expt 3	2694:2699	Depletion of gut microbiota in the YBG group of mice caused diminished fecal SCFA concentrations (Expt 3) and intestinal Treg frequencies (Expt 4).
31879786	14	30	theme	SCFA	2673:2676	arg1	concentrations					2678:2691	diminished fecal SCFA concentrations	2656:2691	diminished fecal SCFA concentrations (Expt 3)	2656:2700	Depletion of gut microbiota in the YBG group of mice caused diminished fecal SCFA concentrations (Expt 3) and intestinal Treg frequencies (Expt 4).
31879786	10	31	dep	%	1940:1940	arg1	P < 0.001					1943:1951	P < 0.001	1943:1951	47%; P < 0.001	1938:1951	RESULTS Compared with controls, the YBG group in Expt 1 exhibited suppressive effects on features of colitis, such as loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01).
31879786	7	32	theme	immune	1355:1360	arg1	phenotype					1362:1370	gut immune phenotype	1351:1370	gut immune phenotype	1351:1370	Expt 3: female B6 mice were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30, and gut immune phenotype and fecal SCFA concentrations were determined.
31879786	0	33	theme	Fatty	172:176	arg1	Acid					178:181	Regulatory Short-Chain Fatty Acid	149:181	Immune Regulatory Short-Chain Fatty Acid Production in C57BL/6 Mice	142:208	Pretreatment with Yeast-Derived Complex Dietary Polysaccharides Suppresses Gut Inflammation, Alters the Microbiota Composition, and Increases Immune Regulatory Short-Chain Fatty Acid Production in C57BL/6 Mice.
31879786	4	34	dep	METHODS	610:616	arg1	mice					646:649	Eight-week-old C57BL/6 (B6) mice	618:649	METHODS Eight-week-old C57BL/6 (B6) mice	610:649	METHODS Eight-week-old C57BL/6 (B6) mice were used in a series of experiments.
31879786	14	35	theme	diminished	2656:2665	arg1	Expt					2694:2697	Expt 3	2694:2699	Expt 3	2694:2699	Depletion of gut microbiota in the YBG group of mice caused diminished fecal SCFA concentrations (Expt 3) and intestinal Treg frequencies (Expt 4).
31879786	14	35	theme	diminished	2656:2665	arg1	concentrations					2678:2691	diminished fecal SCFA concentrations	2656:2691	diminished fecal SCFA concentrations (Expt 3)	2656:2700	Depletion of gut microbiota in the YBG group of mice caused diminished fecal SCFA concentrations (Expt 3) and intestinal Treg frequencies (Expt 4).
31879786	5	36	theme	2.5	814:816	arg1	%					817:817	%	817:817	%	817:817	Experiment (Expt) 1: male and female mice were treated every day, for 40 d, with saline (control) or 250 μg YBG, followed by 2.5% (wt:vol) dextran sulfate sodium (DSS) in drinking water during days 30-35; and colitis severity and intestinal immune phenotype were determined.
31879786	6	37	theme	intestinal	1032:1041	arg1	phenotype					1050:1058	intestinal immune phenotype	1032:1058	intestinal immune phenotype	1032:1058	Expt 2: female B6 mice were treated with saline or YBG for 30 d and intestinal immune phenotype, gut microbiota composition, and fecal SCFA concentrations were determined.
31879786	9	38	theme	drinking	1661:1668	arg1	water					1670:1674	drinking water	1661:1674	drinking water with or without antibiotics during days 16-40	1661:1720	Expt 5: female mice were treated as in Expt 1, given drinking water with or without antibiotics during days 16-40, and colitis severity and intestinal cytokine production were determined.
31879786	8	39	dep	Expt	1419:1422	arg1	treated					1495:1501	treated	1495:1501	were treated as in Expt 3	1490:1514	Expt 4: female B6 Foxp3-green fluorescent protein (-GFP) reporter mice were treated as in Expt 3, and intestinal T-regulatory cell (Treg) frequencies and immune phenotypes were determined.
31879786	8	39	dep	Expt	1419:1422	arg1	determined					1596:1605	determined	1596:1605	were determined	1591:1605	Expt 4: female B6 Foxp3-green fluorescent protein (-GFP) reporter mice were treated as in Expt 3, and intestinal T-regulatory cell (Treg) frequencies and immune phenotypes were determined.
31879786	5	40	theme	colitis	898:904	arg1	severity					906:913	colitis severity	898:913	colitis severity	898:913	Experiment (Expt) 1: male and female mice were treated every day, for 40 d, with saline (control) or 250 μg YBG, followed by 2.5% (wt:vol) dextran sulfate sodium (DSS) in drinking water during days 30-35; and colitis severity and intestinal immune phenotype were determined.
31879786	14	41	theme	mice	2644:2647	arg1	mice					2644:2647	mice	2644:2647	mice	2644:2647	Depletion of gut microbiota in the YBG group of mice caused diminished fecal SCFA concentrations (Expt 3) and intestinal Treg frequencies (Expt 4).
31879786	14	41	theme	mice	2644:2647	arg1	group					2635:2639	the YBG group	2627:2639	the YBG group of mice	2627:2647	Depletion of gut microbiota in the YBG group of mice caused diminished fecal SCFA concentrations (Expt 3) and intestinal Treg frequencies (Expt 4).
31879786	15	42	theme	Expt	2796:2799	arg1	Expt					2796:2799	Expt 5	2796:2801	Expt 5	2796:2801	Compared with the YBG group, the YBG-(Abx) group of Expt 5 showed aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04).
31879786	15	42	theme	Expt	2796:2799	arg1	group					2787:2791	the YBG-(Abx) group	2773:2791	the YBG-(Abx) group of Expt 5	2773:2801	Compared with the YBG group, the YBG-(Abx) group of Expt 5 showed aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04).
31879786	7	43	dep	[Abx	1235:1238	arg1	ampicillin					1241:1250	ampicillin	1241:1250	ampicillin (1 g/L)	1241:1258	Expt 3: female B6 mice were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30, and gut immune phenotype and fecal SCFA concentrations were determined.
31879786	7	43	dep	[Abx	1235:1238	arg1	1 g/L					1253:1257	1 g/L	1253:1257	1 g/L	1253:1257	Expt 3: female B6 mice were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30, and gut immune phenotype and fecal SCFA concentrations were determined.
31879786	7	43	dep	[Abx	1235:1238	arg1	neomycin					1283:1290	neomycin	1283:1290	neomycin (1 g/L)	1283:1298	Expt 3: female B6 mice were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30, and gut immune phenotype and fecal SCFA concentrations were determined.
31879786	7	43	dep	[Abx	1235:1238	arg1	metronidazole					1305:1317	metronidazole	1305:1317	metronidazole	1305:1317	Expt 3: female B6 mice were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30, and gut immune phenotype and fecal SCFA concentrations were determined.
31879786	7	43	dep	[Abx	1235:1238	arg1	1 g/L					1320:1324	1 g/L	1320:1324	1 g/L	1320:1324	Expt 3: female B6 mice were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30, and gut immune phenotype and fecal SCFA concentrations were determined.
31879786	7	43	dep	[Abx	1235:1238	arg1	vancomycin					1261:1270	vancomycin	1261:1270	vancomycin (0.5 g/L)	1261:1280	Expt 3: female B6 mice were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30, and gut immune phenotype and fecal SCFA concentrations were determined.
31879786	7	43	dep	[Abx	1235:1238	arg1	1 g/L					1293:1297	1 g/L	1293:1297	1 g/L	1293:1297	Expt 3: female B6 mice were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30, and gut immune phenotype and fecal SCFA concentrations were determined.
31879786	7	43	dep	[Abx	1235:1238	arg1	0.5 g/L					1273:1279	0.5 g/L	1273:1279	0.5 g/L	1273:1279	Expt 3: female B6 mice were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30, and gut immune phenotype and fecal SCFA concentrations were determined.
31879786	5	44	dep	Experiment	689:698	arg1	treated					736:742	treated	736:742	treated	736:742	Experiment (Expt) 1: male and female mice were treated every day, for 40 d, with saline (control) or 250 μg YBG, followed by 2.5% (wt:vol) dextran sulfate sodium (DSS) in drinking water during days 30-35; and colitis severity and intestinal immune phenotype were determined.
31879786	11	45	theme	microbiota	2113:2122	arg1	abundance					2096:2104	the abundance	2092:2104	the abundance of gut microbiota	2092:2122	The YBG group of Expt 2 showed a shift in the abundance of gut microbiota towards Bacteroides (by 16%; P = 0.049) and Verrucomicrobia (mean ± SD: control = 7.8 ± 0.44 vs. YBG = 21.0 ± 9.6%) and a reduction in Firmicutes (by 66%; P < 0.001).
31879786	12	46	dep	butyric	2439:2445	arg1	%					2453:2453	57%	2451:2453	57%; P = 0.013	2451:2464	The YBG group also showed significantly higher concentrations of fecal SCFAs such as acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids.
31879786	13	47	dep	%	2563:2563	arg1	P = 0.043					2566:2574	P = 0.043	2566:2574	by 32%; P = 0.043	2558:2574	Compared with controls, the YBG group of Expt 2 showed higher frequencies of Tregs (by 32%; P = 0.043) in the gut mucosa.
31879786	11	48	theme	Expt	2067:2070	arg1	Expt					2067:2070	Expt 2	2067:2072	Expt 2	2067:2072	The YBG group of Expt 2 showed a shift in the abundance of gut microbiota towards Bacteroides (by 16%; P = 0.049) and Verrucomicrobia (mean ± SD: control = 7.8 ± 0.44 vs. YBG = 21.0 ± 9.6%) and a reduction in Firmicutes (by 66%; P < 0.001).
31879786	11	48	theme	Expt	2067:2070	arg1	group					2058:2062	The YBG group	2050:2062	The YBG group of Expt 2	2050:2072	The YBG group of Expt 2 showed a shift in the abundance of gut microbiota towards Bacteroides (by 16%; P = 0.049) and Verrucomicrobia (mean ± SD: control = 7.8 ± 0.44 vs. YBG = 21.0 ± 9.6%) and a reduction in Firmicutes (by 66%; P < 0.001).
31879786	5	49	dep	wt	820:821	arg1	vol					823:825	vol	823:825	wt:vol	820:825	Experiment (Expt) 1: male and female mice were treated every day, for 40 d, with saline (control) or 250 μg YBG, followed by 2.5% (wt:vol) dextran sulfate sodium (DSS) in drinking water during days 30-35; and colitis severity and intestinal immune phenotype were determined.
31879786	8	50	theme	intestinal	1521:1530	arg1	Treg					1551:1554	Treg	1551:1554	Treg	1551:1554	Expt 4: female B6 Foxp3-green fluorescent protein (-GFP) reporter mice were treated as in Expt 3, and intestinal T-regulatory cell (Treg) frequencies and immune phenotypes were determined.
31879786	8	50	theme	intestinal	1521:1530	arg1	cell					1545:1548	intestinal T-regulatory cell	1521:1548	intestinal T-regulatory cell (Treg) frequencies	1521:1567	Expt 4: female B6 Foxp3-green fluorescent protein (-GFP) reporter mice were treated as in Expt 3, and intestinal T-regulatory cell (Treg) frequencies and immune phenotypes were determined.
31879786	5	51	dep	%	817:817	arg1	wt					820:821	wt	820:821	wt:vol	820:825	Experiment (Expt) 1: male and female mice were treated every day, for 40 d, with saline (control) or 250 μg YBG, followed by 2.5% (wt:vol) dextran sulfate sodium (DSS) in drinking water during days 30-35; and colitis severity and intestinal immune phenotype were determined.
31879786	0	52	theme	Microbiota	104:113	arg1	Composition					115:125	the Microbiota Composition	100:125	the Microbiota Composition	100:125	Pretreatment with Yeast-Derived Complex Dietary Polysaccharides Suppresses Gut Inflammation, Alters the Microbiota Composition, and Increases Immune Regulatory Short-Chain Fatty Acid Production in C57BL/6 Mice.
31879786	16	53	theme	gut	3052:3054	arg1	intact					3070:3075	intact	3070:3075	intact	3070:3075	CONCLUSIONS Studies using B6 mice show that dietary BGs are beneficial for promoting intestinal health when the gut microbiota is intact.
31879786	16	53	theme	gut	3052:3054	arg1	microbiota					3056:3065	the gut microbiota	3048:3065	the gut microbiota	3048:3065	CONCLUSIONS Studies using B6 mice show that dietary BGs are beneficial for promoting intestinal health when the gut microbiota is intact.
31879786	8	54	theme	cell	1545:1548	arg1	frequencies					1557:1567	intestinal T-regulatory cell (Treg) frequencies	1521:1567	intestinal T-regulatory cell (Treg) frequencies	1521:1567	Expt 4: female B6 Foxp3-green fluorescent protein (-GFP) reporter mice were treated as in Expt 3, and intestinal T-regulatory cell (Treg) frequencies and immune phenotypes were determined.
31879786	5	55	theme	sulfate	836:842	arg1	DSS					852:854	2.5% (wt:vol) dextran sulfate sodium (DSS)	814:855	2.5% (wt:vol) dextran sulfate sodium (DSS) in drinking water during days 30-35	814:891	Experiment (Expt) 1: male and female mice were treated every day, for 40 d, with saline (control) or 250 μg YBG, followed by 2.5% (wt:vol) dextran sulfate sodium (DSS) in drinking water during days 30-35; and colitis severity and intestinal immune phenotype were determined.
31879786	15	56	theme	aggravated	2810:2819	arg1	loss					2848:2851	loss	2848:2851	loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04)	2848:2937	Compared with the YBG group, the YBG-(Abx) group of Expt 5 showed aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04).
31879786	15	56	theme	aggravated	2810:2819	arg1	features					2829:2836	aggravated colitis features	2810:2836	aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04)	2810:2937	Compared with the YBG group, the YBG-(Abx) group of Expt 5 showed aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04).
31879786	10	57	theme	severity	2015:2022	arg1	score					2024:2028	histopathology severity score	2000:2028	histopathology severity score (by 45%; P = 0.01)	2000:2047	RESULTS Compared with controls, the YBG group in Expt 1 exhibited suppressive effects on features of colitis, such as loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01).
31879786	10	58	theme	colitis	1897:1903	arg1	features					1885:1892	features	1885:1892	features	1885:1892	RESULTS Compared with controls, the YBG group in Expt 1 exhibited suppressive effects on features of colitis, such as loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01).
31879786	10	58	theme	colitis	1897:1903	arg1	loss					1914:1917	loss	1914:1917	loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01)	1914:2047	RESULTS Compared with controls, the YBG group in Expt 1 exhibited suppressive effects on features of colitis, such as loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01).
31879786	2	59	dep	administered	359:370	arg1	purified					379:386	purified	379:386	purified	379:386	However, the effects of orally administered highly purified BGs on gut inflammation are largely unknown.
31879786	12	60	theme	butyric	2439:2445	arg1	acids					2467:2471	acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids	2376:2471	acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids	2376:2471	The YBG group also showed significantly higher concentrations of fecal SCFAs such as acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids.
31879786	9	61	dep	Expt	1608:1611	arg1	determined					1784:1793	determined	1784:1793	were determined	1779:1793	Expt 5: female mice were treated as in Expt 1, given drinking water with or without antibiotics during days 16-40, and colitis severity and intestinal cytokine production were determined.
31879786	9	61	dep	Expt	1608:1611	arg1	treated					1633:1639	treated	1633:1639	treated as in Expt 1	1633:1652	Expt 5: female mice were treated as in Expt 1, given drinking water with or without antibiotics during days 16-40, and colitis severity and intestinal cytokine production were determined.
31879786	15	62	dep	%	2926:2926	arg1	P = 0.04					2929:2936	P = 0.04	2929:2936	42%; P = 0.04	2924:2936	Compared with the YBG group, the YBG-(Abx) group of Expt 5 showed aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04).
31879786	10	63	theme	body	1922:1925	arg1	weight					1927:1932	body weight	1922:1932	body weight (by 47%; P < 0.001)	1922:1952	RESULTS Compared with controls, the YBG group in Expt 1 exhibited suppressive effects on features of colitis, such as loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01).
31879786	10	64	dep	%	2036:2036	arg1	P = 0.01					2039:2046	P = 0.01	2039:2046	45%; P = 0.01	2034:2046	RESULTS Compared with controls, the YBG group in Expt 1 exhibited suppressive effects on features of colitis, such as loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01).
31879786	7	65	theme	female	1144:1149	arg1	mice					1154:1157	female B6 mice	1144:1157	female B6 mice	1144:1157	Expt 3: female B6 mice were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30, and gut immune phenotype and fecal SCFA concentrations were determined.
31879786	12	66	theme	SCFAs	2362:2366	arg1	acids					2467:2471	acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids	2376:2471	acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids	2376:2471	The YBG group also showed significantly higher concentrations of fecal SCFAs such as acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids.
31879786	12	66	theme	SCFAs	2362:2366	arg1	concentrations					2338:2351	significantly higher concentrations	2317:2351	significantly higher concentrations of fecal SCFAs such as acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids	2317:2471	The YBG group also showed significantly higher concentrations of fecal SCFAs such as acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids.
31879786	0	67	theme	Yeast-Derived	18:30	arg1	Polysaccharides					48:62	Yeast-Derived Complex Dietary Polysaccharides	18:62	Yeast-Derived Complex Dietary Polysaccharides	18:62	Pretreatment with Yeast-Derived Complex Dietary Polysaccharides Suppresses Gut Inflammation, Alters the Microbiota Composition, and Increases Immune Regulatory Short-Chain Fatty Acid Production in C57BL/6 Mice.
31879786	16	68	theme	dietary	2984:2990	arg1	BGs					2992:2994	dietary BGs	2984:2994	dietary BGs	2984:2994	CONCLUSIONS Studies using B6 mice show that dietary BGs are beneficial for promoting intestinal health when the gut microbiota is intact.
31879786	14	69	from	Depletion	2596:2604	arg1	mice					2644:2647	mice	2644:2647	mice	2644:2647	Depletion of gut microbiota in the YBG group of mice caused diminished fecal SCFA concentrations (Expt 3) and intestinal Treg frequencies (Expt 4).
31879786	14	69	from	Depletion	2596:2604	arg1	group					2635:2639	the YBG group	2627:2639	the YBG group of mice	2627:2647	Depletion of gut microbiota in the YBG group of mice caused diminished fecal SCFA concentrations (Expt 3) and intestinal Treg frequencies (Expt 4).
31879786	9	70	theme	intestinal	1748:1757	arg1	production					1768:1777	intestinal cytokine production	1748:1777	intestinal cytokine production	1748:1777	Expt 5: female mice were treated as in Expt 1, given drinking water with or without antibiotics during days 16-40, and colitis severity and intestinal cytokine production were determined.
31879786	0	71	theme	Dietary	40:46	arg1	Polysaccharides					48:62	Yeast-Derived Complex Dietary Polysaccharides	18:62	Yeast-Derived Complex Dietary Polysaccharides	18:62	Pretreatment with Yeast-Derived Complex Dietary Polysaccharides Suppresses Gut Inflammation, Alters the Microbiota Composition, and Increases Immune Regulatory Short-Chain Fatty Acid Production in C57BL/6 Mice.
31879786	12	72	theme	higher	2331:2336	arg1	acids					2467:2471	acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids	2376:2471	acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids	2376:2471	The YBG group also showed significantly higher concentrations of fecal SCFAs such as acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids.
31879786	12	72	theme	higher	2331:2336	arg1	concentrations					2338:2351	significantly higher concentrations	2317:2351	significantly higher concentrations of fecal SCFAs such as acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids	2317:2471	The YBG group also showed significantly higher concentrations of fecal SCFAs such as acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids.
31879786	6	73	dep	Expt	964:967	arg1	treated					992:998	treated	992:998	were treated with saline or YBG for 30 d	987:1026	Expt 2: female B6 mice were treated with saline or YBG for 30 d and intestinal immune phenotype, gut microbiota composition, and fecal SCFA concentrations were determined.
31879786	6	73	dep	Expt	964:967	arg1	determined					1124:1133	determined	1124:1133	were determined	1119:1133	Expt 2: female B6 mice were treated with saline or YBG for 30 d and intestinal immune phenotype, gut microbiota composition, and fecal SCFA concentrations were determined.
31879786	1	74	theme	dietary	307:313	arg1	supplements					315:325	dietary supplements	307:325	dietary supplements	307:325	BACKGROUND β-Glucans (BGs), a group of complex dietary polysaccharides (CDPs), are available as dietary supplements.
31879786	12	75	theme	acetic	2376:2381	arg1	acids					2467:2471	acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids	2376:2471	acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids	2376:2471	The YBG group also showed significantly higher concentrations of fecal SCFAs such as acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids.
31879786	6	76	theme	female	972:977	arg1	mice					982:985	female B6 mice	972:985	female B6 mice	972:985	Expt 2: female B6 mice were treated with saline or YBG for 30 d and intestinal immune phenotype, gut microbiota composition, and fecal SCFA concentrations were determined.
31879786	0	77	with	Pretreatment	0:11	arg1	Polysaccharides					48:62	Yeast-Derived Complex Dietary Polysaccharides	18:62	Yeast-Derived Complex Dietary Polysaccharides	18:62	Pretreatment with Yeast-Derived Complex Dietary Polysaccharides Suppresses Gut Inflammation, Alters the Microbiota Composition, and Increases Immune Regulatory Short-Chain Fatty Acid Production in C57BL/6 Mice.
31879786	13	78	dep	Tregs	2551:2555	arg1	%					2563:2563	by 32%	2558:2563	by 32%; P = 0.043	2558:2574	Compared with controls, the YBG group of Expt 2 showed higher frequencies of Tregs (by 32%; P = 0.043) in the gut mucosa.
31879786	17	79	theme	adverse	3110:3116	arg1	effects					3118:3124	adverse effects	3110:3124	adverse effects	3110:3124	However, these CDPs may produce adverse effects if gut microbiota is compromised.
31879786	13	80	theme	by	2558:2559	arg1	%					2563:2563	by 32%	2558:2563	by 32%; P = 0.043	2558:2574	Compared with controls, the YBG group of Expt 2 showed higher frequencies of Tregs (by 32%; P = 0.043) in the gut mucosa.
31879786	2	81	theme	administered	359:370	arg1	BGs					388:390	orally administered highly purified BGs	352:390	orally administered highly purified BGs	352:390	However, the effects of orally administered highly purified BGs on gut inflammation are largely unknown.
31879786	15	82	theme	by	2869:2870	arg1	%					2876:2876	by >100%	2869:2876	by >100%; P < 0.01	2869:2886	Compared with the YBG group, the YBG-(Abx) group of Expt 5 showed aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04).
31879786	11	83	dep	Verrucomicrobia	2168:2182	arg1	mean ± SD					2185:2193	mean ± SD	2185:2193	mean ± SD: control = 7.8 ± 0.44 vs. YBG = 21.0 ± 9.6%	2185:2237	The YBG group of Expt 2 showed a shift in the abundance of gut microbiota towards Bacteroides (by 16%; P = 0.049) and Verrucomicrobia (mean ± SD: control = 7.8 ± 0.44 vs. YBG = 21.0 ± 9.6%) and a reduction in Firmicutes (by 66%; P < 0.001).
31879786	14	84	theme	fecal	2667:2671	arg1	Expt					2694:2697	Expt 3	2694:2699	Expt 3	2694:2699	Depletion of gut microbiota in the YBG group of mice caused diminished fecal SCFA concentrations (Expt 3) and intestinal Treg frequencies (Expt 4).
31879786	14	84	theme	fecal	2667:2671	arg1	concentrations					2678:2691	diminished fecal SCFA concentrations	2656:2691	diminished fecal SCFA concentrations (Expt 3)	2656:2700	Depletion of gut microbiota in the YBG group of mice caused diminished fecal SCFA concentrations (Expt 3) and intestinal Treg frequencies (Expt 4).
31879786	8	85	theme	immune	1573:1578	arg1	phenotypes					1580:1589	immune phenotypes	1573:1589	immune phenotypes	1573:1589	Expt 4: female B6 Foxp3-green fluorescent protein (-GFP) reporter mice were treated as in Expt 3, and intestinal T-regulatory cell (Treg) frequencies and immune phenotypes were determined.
31879786	12	86	theme	propionic	2404:2412	arg1	acids					2467:2471	acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids	2376:2471	acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids	2376:2471	The YBG group also showed significantly higher concentrations of fecal SCFAs such as acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids.
31879786	1	87	theme	complex	250:256	arg1	CDPs					283:286	CDPs	283:286	CDPs	283:286	BACKGROUND β-Glucans (BGs), a group of complex dietary polysaccharides (CDPs), are available as dietary supplements.
31879786	1	87	theme	complex	250:256	arg1	polysaccharides					266:280	complex dietary polysaccharides	250:280	complex dietary polysaccharides (CDPs)	250:287	BACKGROUND β-Glucans (BGs), a group of complex dietary polysaccharides (CDPs), are available as dietary supplements.
31879786	10	88	dep	%	1981:1981	arg1	P = 0.016					1984:1992	P = 0.016	1984:1992	24%; P = 0.016	1979:1992	RESULTS Compared with controls, the YBG group in Expt 1 exhibited suppressive effects on features of colitis, such as loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01).
31879786	11	89	dep	mean ± SD	2185:2193	arg1	%					2237:2237	control = 7.8 ± 0.44 vs. YBG = 21.0 ± 9.6%	2196:2237	mean ± SD: control = 7.8 ± 0.44 vs. YBG = 21.0 ± 9.6%	2185:2237	The YBG group of Expt 2 showed a shift in the abundance of gut microbiota towards Bacteroides (by 16%; P = 0.049) and Verrucomicrobia (mean ± SD: control = 7.8 ± 0.44 vs. YBG = 21.0 ± 9.6%) and a reduction in Firmicutes (by 66%; P < 0.001).
31879786	0	90	from	Production	183:192	arg1	Mice					205:208	C57BL/6 Mice	197:208	C57BL/6 Mice	197:208	Pretreatment with Yeast-Derived Complex Dietary Polysaccharides Suppresses Gut Inflammation, Alters the Microbiota Composition, and Increases Immune Regulatory Short-Chain Fatty Acid Production in C57BL/6 Mice.
31879786	6	91	theme	SCFA	1099:1102	arg1	concentrations					1104:1117	fecal SCFA concentrations	1093:1117	fecal SCFA concentrations	1093:1117	Expt 2: female B6 mice were treated with saline or YBG for 30 d and intestinal immune phenotype, gut microbiota composition, and fecal SCFA concentrations were determined.
31879786	1	92	theme	polysaccharides	266:280	arg1	group					241:245	a group	239:245	a group of complex dietary polysaccharides (CDPs)	239:287	BACKGROUND β-Glucans (BGs), a group of complex dietary polysaccharides (CDPs), are available as dietary supplements.
31879786	1	92	theme	polysaccharides	266:280	arg1	CDPs					283:286	CDPs	283:286	CDPs	283:286	BACKGROUND β-Glucans (BGs), a group of complex dietary polysaccharides (CDPs), are available as dietary supplements.
31879786	1	92	theme	polysaccharides	266:280	arg1	polysaccharides					266:280	complex dietary polysaccharides	250:280	complex dietary polysaccharides (CDPs)	250:287	BACKGROUND β-Glucans (BGs), a group of complex dietary polysaccharides (CDPs), are available as dietary supplements.
31879786	1	92	theme	polysaccharides	266:280	arg1	β-Glucans					222:230	BACKGROUND β-Glucans	211:230	BACKGROUND β-Glucans (BGs)	211:236	BACKGROUND β-Glucans (BGs), a group of complex dietary polysaccharides (CDPs), are available as dietary supplements.
31879786	12	93	theme	YBG	2295:2297	arg1	group					2299:2303	The YBG group	2291:2303	The YBG group	2291:2303	The YBG group also showed significantly higher concentrations of fecal SCFAs such as acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids.
31879786	8	94	theme	B6	1434:1435	arg1	mice					1485:1488	female B6 Foxp3-green fluorescent protein (-GFP) reporter mice	1427:1488	female B6 Foxp3-green fluorescent protein (-GFP) reporter mice	1427:1488	Expt 4: female B6 Foxp3-green fluorescent protein (-GFP) reporter mice were treated as in Expt 3, and intestinal T-regulatory cell (Treg) frequencies and immune phenotypes were determined.
31879786	15	95	dep	%	2876:2876	arg1	P < 0.01					2879:2886	P < 0.01	2879:2886	by >100%; P < 0.01	2869:2886	Compared with the YBG group, the YBG-(Abx) group of Expt 5 showed aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04).
31879786	3	96	dep	purified	527:534	arg1	yeast-derived					537:549	yeast-derived	537:549	yeast-derived	537:549	OBJECTIVES The aim of this study was to investigate the impact of orally administering highly purified, yeast-derived BG (YBG; β-1,3/1,6-d-glucan) on susceptibility to colitis.
31879786	13	97	theme	YBG	2502:2504	arg1	group					2506:2510	the YBG group	2498:2510	the YBG group of Expt 2	2498:2520	Compared with controls, the YBG group of Expt 2 showed higher frequencies of Tregs (by 32%; P = 0.043) in the gut mucosa.
31879786	13	97	theme	YBG	2502:2504	arg1	Expt					2515:2518	Expt 2	2515:2520	Expt 2	2515:2520	Compared with controls, the YBG group of Expt 2 showed higher frequencies of Tregs (by 32%; P = 0.043) in the gut mucosa.
31879786	8	98	theme	fluorescent	1449:1459	arg1	mice					1485:1488	female B6 Foxp3-green fluorescent protein (-GFP) reporter mice	1427:1488	female B6 Foxp3-green fluorescent protein (-GFP) reporter mice	1427:1488	Expt 4: female B6 Foxp3-green fluorescent protein (-GFP) reporter mice were treated as in Expt 3, and intestinal T-regulatory cell (Treg) frequencies and immune phenotypes were determined.
31879786	15	99	theme	inflammation	2901:2912	arg1	score					2914:2918	colonic inflammation score	2893:2918	colonic inflammation score (by 42%; P = 0.04)	2893:2937	Compared with the YBG group, the YBG-(Abx) group of Expt 5 showed aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04).
31879786	7	100	theme	fecal	1376:1380	arg1	concentrations					1387:1400	fecal SCFA concentrations	1376:1400	fecal SCFA concentrations	1376:1400	Expt 3: female B6 mice were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30, and gut immune phenotype and fecal SCFA concentrations were determined.
31879786	11	101	from	reduction	2246:2254	arg1	abundance					2096:2104	the abundance	2092:2104	the abundance of gut microbiota	2092:2122	The YBG group of Expt 2 showed a shift in the abundance of gut microbiota towards Bacteroides (by 16%; P = 0.049) and Verrucomicrobia (mean ± SD: control = 7.8 ± 0.44 vs. YBG = 21.0 ± 9.6%) and a reduction in Firmicutes (by 66%; P < 0.001).
31879786	11	101	from	reduction	2246:2254	arg1	Firmicutes					2259:2268	Firmicutes	2259:2268	Firmicutes	2259:2268	The YBG group of Expt 2 showed a shift in the abundance of gut microbiota towards Bacteroides (by 16%; P = 0.049) and Verrucomicrobia (mean ± SD: control = 7.8 ± 0.44 vs. YBG = 21.0 ± 9.6%) and a reduction in Firmicutes (by 66%; P < 0.001).
31879786	0	102	theme	Immune	142:147	arg1	Production					183:192	Immune Regulatory Short-Chain Fatty Acid Production	142:192	Immune Regulatory Short-Chain Fatty Acid Production in C57BL/6 Mice	142:208	Pretreatment with Yeast-Derived Complex Dietary Polysaccharides Suppresses Gut Inflammation, Alters the Microbiota Composition, and Increases Immune Regulatory Short-Chain Fatty Acid Production in C57BL/6 Mice.
31879786	11	103	from	shift	2083:2087	arg1	abundance					2096:2104	the abundance	2092:2104	the abundance of gut microbiota	2092:2122	The YBG group of Expt 2 showed a shift in the abundance of gut microbiota towards Bacteroides (by 16%; P = 0.049) and Verrucomicrobia (mean ± SD: control = 7.8 ± 0.44 vs. YBG = 21.0 ± 9.6%) and a reduction in Firmicutes (by 66%; P < 0.001).
31879786	11	103	from	shift	2083:2087	arg1	Firmicutes					2259:2268	Firmicutes	2259:2268	Firmicutes	2259:2268	The YBG group of Expt 2 showed a shift in the abundance of gut microbiota towards Bacteroides (by 16%; P = 0.049) and Verrucomicrobia (mean ± SD: control = 7.8 ± 0.44 vs. YBG = 21.0 ± 9.6%) and a reduction in Firmicutes (by 66%; P < 0.001).
31879786	10	104	theme	colon	1969:1973	arg1	shortening					1955:1964	shortening	1955:1964	shortening of colon (by 24%; P = 0.016)	1955:1993	RESULTS Compared with controls, the YBG group in Expt 1 exhibited suppressive effects on features of colitis, such as loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01).
31879786	10	104	theme	colon	1969:1973	arg1	score					2024:2028	histopathology severity score	2000:2028	histopathology severity score (by 45%; P = 0.01)	2000:2047	RESULTS Compared with controls, the YBG group in Expt 1 exhibited suppressive effects on features of colitis, such as loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01).
31879786	10	104	theme	colon	1969:1973	arg1	weight					1927:1932	body weight	1922:1932	body weight (by 47%; P < 0.001)	1922:1952	RESULTS Compared with controls, the YBG group in Expt 1 exhibited suppressive effects on features of colitis, such as loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01).
31879786	10	105	theme	YBG	1832:1834	arg1	group					1836:1840	the YBG group	1828:1840	the YBG group in Expt 1	1828:1850	RESULTS Compared with controls, the YBG group in Expt 1 exhibited suppressive effects on features of colitis, such as loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01).
31879786	4	106	theme	Eight-week-old	618:631	arg1	mice					646:649	Eight-week-old C57BL/6 (B6) mice	618:649	METHODS Eight-week-old C57BL/6 (B6) mice	610:649	METHODS Eight-week-old C57BL/6 (B6) mice were used in a series of experiments.
31879786	13	107	theme	Tregs	2551:2555	arg1	frequencies					2536:2546	higher frequencies	2529:2546	higher frequencies of Tregs (by 32%; P = 0.043)	2529:2575	Compared with controls, the YBG group of Expt 2 showed higher frequencies of Tregs (by 32%; P = 0.043) in the gut mucosa.
31879786	0	108	theme	Short-Chain	160:170	arg1	Acid					178:181	Regulatory Short-Chain Fatty Acid	149:181	Immune Regulatory Short-Chain Fatty Acid Production in C57BL/6 Mice	142:208	Pretreatment with Yeast-Derived Complex Dietary Polysaccharides Suppresses Gut Inflammation, Alters the Microbiota Composition, and Increases Immune Regulatory Short-Chain Fatty Acid Production in C57BL/6 Mice.
31879786	5	109	theme	intestinal	919:928	arg1	phenotype					937:945	intestinal immune phenotype	919:945	intestinal immune phenotype	919:945	Experiment (Expt) 1: male and female mice were treated every day, for 40 d, with saline (control) or 250 μg YBG, followed by 2.5% (wt:vol) dextran sulfate sodium (DSS) in drinking water during days 30-35; and colitis severity and intestinal immune phenotype were determined.
31879786	5	110	theme	250	790:792	arg1	μg					794:795	μg	794:795	μg	794:795	Experiment (Expt) 1: male and female mice were treated every day, for 40 d, with saline (control) or 250 μg YBG, followed by 2.5% (wt:vol) dextran sulfate sodium (DSS) in drinking water during days 30-35; and colitis severity and intestinal immune phenotype were determined.
31879786	7	111	theme	gut	1351:1353	arg1	phenotype					1362:1370	gut immune phenotype	1351:1370	gut immune phenotype	1351:1370	Expt 3: female B6 mice were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30, and gut immune phenotype and fecal SCFA concentrations were determined.
31879786	10	112	theme	shortening	1955:1964	arg1	loss					1914:1917	loss	1914:1917	loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01)	1914:2047	RESULTS Compared with controls, the YBG group in Expt 1 exhibited suppressive effects on features of colitis, such as loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01).
31879786	7	113	theme	given	1186:1190	arg1	water					1201:1205	given drinking water	1186:1205	given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30	1186:1344	Expt 3: female B6 mice were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30, and gut immune phenotype and fecal SCFA concentrations were determined.
31879786	10	114	theme	suppressive	1862:1872	arg1	effects					1874:1880	suppressive effects	1862:1880	suppressive effects on features of colitis, such as loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01)	1862:2047	RESULTS Compared with controls, the YBG group in Expt 1 exhibited suppressive effects on features of colitis, such as loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01).
31879786	0	115	theme	Acid	178:181	arg1	Production					183:192	Immune Regulatory Short-Chain Fatty Acid Production	142:192	Immune Regulatory Short-Chain Fatty Acid Production in C57BL/6 Mice	142:208	Pretreatment with Yeast-Derived Complex Dietary Polysaccharides Suppresses Gut Inflammation, Alters the Microbiota Composition, and Increases Immune Regulatory Short-Chain Fatty Acid Production in C57BL/6 Mice.
31879786	16	116	theme	intestinal	3025:3034	arg1	health					3036:3041	intestinal health	3025:3041	intestinal health	3025:3041	CONCLUSIONS Studies using B6 mice show that dietary BGs are beneficial for promoting intestinal health when the gut microbiota is intact.
31879786	4	117	used	used	656:659	arg2	METHODS					610:616	METHODS	610:616	METHODS Eight-week-old C57BL/6 (B6) mice	610:649	METHODS Eight-week-old C57BL/6 (B6) mice were used in a series of experiments.
31879786	6	118	theme	immune	1043:1048	arg1	phenotype					1050:1058	intestinal immune phenotype	1032:1058	intestinal immune phenotype	1032:1058	Expt 2: female B6 mice were treated with saline or YBG for 30 d and intestinal immune phenotype, gut microbiota composition, and fecal SCFA concentrations were determined.
31879786	4	119	theme	experiments	676:686	arg1	series					666:671	a series	664:671	a series of experiments	664:686	METHODS Eight-week-old C57BL/6 (B6) mice were used in a series of experiments.
31879786	5	120	theme	%	817:817	arg1	DSS					852:854	2.5% (wt:vol) dextran sulfate sodium (DSS)	814:855	2.5% (wt:vol) dextran sulfate sodium (DSS) in drinking water during days 30-35	814:891	Experiment (Expt) 1: male and female mice were treated every day, for 40 d, with saline (control) or 250 μg YBG, followed by 2.5% (wt:vol) dextran sulfate sodium (DSS) in drinking water during days 30-35; and colitis severity and intestinal immune phenotype were determined.
31879786	15	121	theme	Abx	2782:2784	arg1	Expt					2796:2799	Expt 5	2796:2801	Expt 5	2796:2801	Compared with the YBG group, the YBG-(Abx) group of Expt 5 showed aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04).
31879786	15	121	theme	Abx	2782:2784	arg1	group					2787:2791	the YBG-(Abx) group	2773:2791	the YBG-(Abx) group of Expt 5	2773:2801	Compared with the YBG group, the YBG-(Abx) group of Expt 5 showed aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04).
31879786	11	122	theme	gut	2109:2111	arg1	microbiota					2113:2122	gut microbiota	2109:2122	gut microbiota	2109:2122	The YBG group of Expt 2 showed a shift in the abundance of gut microbiota towards Bacteroides (by 16%; P = 0.049) and Verrucomicrobia (mean ± SD: control = 7.8 ± 0.44 vs. YBG = 21.0 ± 9.6%) and a reduction in Firmicutes (by 66%; P < 0.001).
31879786	16	123	theme	CONCLUSIONS	2940:2950	arg1	Studies					2952:2958	CONCLUSIONS Studies	2940:2958	CONCLUSIONS Studies using B6 mice	2940:2972	CONCLUSIONS Studies using B6 mice show that dietary BGs are beneficial for promoting intestinal health when the gut microbiota is intact.
31879786	17	124	theme	gut	3129:3131	arg1	microbiota					3133:3142	gut microbiota	3129:3142	gut microbiota	3129:3142	However, these CDPs may produce adverse effects if gut microbiota is compromised.
31879786	5	125	theme	male	710:713	arg1	mice					726:729	male and female mice	710:729	male and female mice	710:729	Experiment (Expt) 1: male and female mice were treated every day, for 40 d, with saline (control) or 250 μg YBG, followed by 2.5% (wt:vol) dextran sulfate sodium (DSS) in drinking water during days 30-35; and colitis severity and intestinal immune phenotype were determined.
31879786	5	126	theme	μg	794:795	arg1	YBG					797:799	250 μg YBG	790:799	250 μg YBG	790:799	Experiment (Expt) 1: male and female mice were treated every day, for 40 d, with saline (control) or 250 μg YBG, followed by 2.5% (wt:vol) dextran sulfate sodium (DSS) in drinking water during days 30-35; and colitis severity and intestinal immune phenotype were determined.
31879786	15	127	theme	YBG-	2777:2780	arg1	Expt					2796:2799	Expt 5	2796:2801	Expt 5	2796:2801	Compared with the YBG group, the YBG-(Abx) group of Expt 5 showed aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04).
31879786	15	127	theme	YBG-	2777:2780	arg1	group					2787:2791	the YBG-(Abx) group	2773:2791	the YBG-(Abx) group of Expt 5	2773:2801	Compared with the YBG group, the YBG-(Abx) group of Expt 5 showed aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04).
31879786	14	128	theme	Treg	2717:2720	arg1	Expt					2735:2738	Expt 4	2735:2740	Expt 4	2735:2740	Depletion of gut microbiota in the YBG group of mice caused diminished fecal SCFA concentrations (Expt 3) and intestinal Treg frequencies (Expt 4).
31879786	14	128	theme	Treg	2717:2720	arg1	frequencies					2722:2732	intestinal Treg frequencies	2706:2732	intestinal Treg frequencies (Expt 4)	2706:2741	Depletion of gut microbiota in the YBG group of mice caused diminished fecal SCFA concentrations (Expt 3) and intestinal Treg frequencies (Expt 4).
31879786	5	129	theme	female	719:724	arg1	mice					726:729	male and female mice	710:729	male and female mice	710:729	Experiment (Expt) 1: male and female mice were treated every day, for 40 d, with saline (control) or 250 μg YBG, followed by 2.5% (wt:vol) dextran sulfate sodium (DSS) in drinking water during days 30-35; and colitis severity and intestinal immune phenotype were determined.
31879786	14	130	theme	YBG	2631:2633	arg1	mice					2644:2647	mice	2644:2647	mice	2644:2647	Depletion of gut microbiota in the YBG group of mice caused diminished fecal SCFA concentrations (Expt 3) and intestinal Treg frequencies (Expt 4).
31879786	14	130	theme	YBG	2631:2633	arg1	group					2635:2639	the YBG group	2627:2639	the YBG group of mice	2627:2647	Depletion of gut microbiota in the YBG group of mice caused diminished fecal SCFA concentrations (Expt 3) and intestinal Treg frequencies (Expt 4).
31879786	3	131	dep	YBG	555:557	arg1	β-1,3/1,6-d-glucan					560:577	β-1,3/1,6-d-glucan	560:577	YBG; β-1,3/1,6-d-glucan	555:577	OBJECTIVES The aim of this study was to investigate the impact of orally administering highly purified, yeast-derived BG (YBG; β-1,3/1,6-d-glucan) on susceptibility to colitis.
31879786	15	132	dep	weight	2861:2866	arg1	%					2876:2876	by >100%	2869:2876	by >100%; P < 0.01	2869:2886	Compared with the YBG group, the YBG-(Abx) group of Expt 5 showed aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04).
31879786	11	133	theme	YBG	2054:2056	arg1	Expt					2067:2070	Expt 2	2067:2072	Expt 2	2067:2072	The YBG group of Expt 2 showed a shift in the abundance of gut microbiota towards Bacteroides (by 16%; P = 0.049) and Verrucomicrobia (mean ± SD: control = 7.8 ± 0.44 vs. YBG = 21.0 ± 9.6%) and a reduction in Firmicutes (by 66%; P < 0.001).
31879786	11	133	theme	YBG	2054:2056	arg1	group					2058:2062	The YBG group	2050:2062	The YBG group of Expt 2	2050:2072	The YBG group of Expt 2 showed a shift in the abundance of gut microbiota towards Bacteroides (by 16%; P = 0.049) and Verrucomicrobia (mean ± SD: control = 7.8 ± 0.44 vs. YBG = 21.0 ± 9.6%) and a reduction in Firmicutes (by 66%; P < 0.001).
31879786	14	134	theme	microbiota	2613:2622	arg1	Depletion					2596:2604	Depletion	2596:2604	Depletion of gut microbiota in the YBG group of mice	2596:2647	Depletion of gut microbiota in the YBG group of mice caused diminished fecal SCFA concentrations (Expt 3) and intestinal Treg frequencies (Expt 4).
31879786	5	135	theme	dextran	828:834	arg1	DSS					852:854	2.5% (wt:vol) dextran sulfate sodium (DSS)	814:855	2.5% (wt:vol) dextran sulfate sodium (DSS) in drinking water during days 30-35	814:891	Experiment (Expt) 1: male and female mice were treated every day, for 40 d, with saline (control) or 250 μg YBG, followed by 2.5% (wt:vol) dextran sulfate sodium (DSS) in drinking water during days 30-35; and colitis severity and intestinal immune phenotype were determined.
31879786	15	136	theme	YBG	2762:2764	arg1	group					2766:2770	the YBG group	2758:2770	the YBG group	2758:2770	Compared with the YBG group, the YBG-(Abx) group of Expt 5 showed aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04).
31879786	10	137	theme	score	2024:2028	arg1	loss					1914:1917	loss	1914:1917	loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01)	1914:2047	RESULTS Compared with controls, the YBG group in Expt 1 exhibited suppressive effects on features of colitis, such as loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01).
31879786	10	138	from	effects	1874:1880	arg1	features					1885:1892	features	1885:1892	features	1885:1892	RESULTS Compared with controls, the YBG group in Expt 1 exhibited suppressive effects on features of colitis, such as loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01).
31879786	10	138	from	effects	1874:1880	arg1	loss					1914:1917	loss	1914:1917	loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01)	1914:2047	RESULTS Compared with controls, the YBG group in Expt 1 exhibited suppressive effects on features of colitis, such as loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01).
31879786	8	139	theme	T-regulatory	1532:1543	arg1	Treg					1551:1554	Treg	1551:1554	Treg	1551:1554	Expt 4: female B6 Foxp3-green fluorescent protein (-GFP) reporter mice were treated as in Expt 3, and intestinal T-regulatory cell (Treg) frequencies and immune phenotypes were determined.
31879786	8	139	theme	T-regulatory	1532:1543	arg1	cell					1545:1548	intestinal T-regulatory cell	1521:1548	intestinal T-regulatory cell (Treg) frequencies	1521:1567	Expt 4: female B6 Foxp3-green fluorescent protein (-GFP) reporter mice were treated as in Expt 3, and intestinal T-regulatory cell (Treg) frequencies and immune phenotypes were determined.
31879786	5	140	theme	sodium	844:849	arg1	DSS					852:854	2.5% (wt:vol) dextran sulfate sodium (DSS)	814:855	2.5% (wt:vol) dextran sulfate sodium (DSS) in drinking water during days 30-35	814:891	Experiment (Expt) 1: male and female mice were treated every day, for 40 d, with saline (control) or 250 μg YBG, followed by 2.5% (wt:vol) dextran sulfate sodium (DSS) in drinking water during days 30-35; and colitis severity and intestinal immune phenotype were determined.
31879786	15	141	theme	colitis	2821:2827	arg1	loss					2848:2851	loss	2848:2851	loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04)	2848:2937	Compared with the YBG group, the YBG-(Abx) group of Expt 5 showed aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04).
31879786	15	141	theme	colitis	2821:2827	arg1	features					2829:2836	aggravated colitis features	2810:2836	aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04)	2810:2937	Compared with the YBG group, the YBG-(Abx) group of Expt 5 showed aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04).
31879786	7	142	theme	B6	1151:1152	arg1	mice					1154:1157	female B6 mice	1144:1157	female B6 mice	1144:1157	Expt 3: female B6 mice were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30, and gut immune phenotype and fecal SCFA concentrations were determined.
31879786	10	143	theme	histopathology	2000:2013	arg1	score					2024:2028	histopathology severity score	2000:2028	histopathology severity score (by 45%; P = 0.01)	2000:2047	RESULTS Compared with controls, the YBG group in Expt 1 exhibited suppressive effects on features of colitis, such as loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01).
31879786	10	144	theme	weight	1927:1932	arg1	loss					1914:1917	loss	1914:1917	loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01)	1914:2047	RESULTS Compared with controls, the YBG group in Expt 1 exhibited suppressive effects on features of colitis, such as loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01).
31879786	2	145	theme	BGs	388:390	arg1	unknown					424:430	unknown	424:430	unknown	424:430	However, the effects of orally administered highly purified BGs on gut inflammation are largely unknown.
31879786	2	145	theme	BGs	388:390	arg1	effects					341:347	the effects	337:347	the effects of orally administered highly purified BGs on gut inflammation	337:410	However, the effects of orally administered highly purified BGs on gut inflammation are largely unknown.
31879786	13	146	theme	Expt	2515:2518	arg1	group					2506:2510	the YBG group	2498:2510	the YBG group of Expt 2	2498:2520	Compared with controls, the YBG group of Expt 2 showed higher frequencies of Tregs (by 32%; P = 0.043) in the gut mucosa.
31879786	13	146	theme	Expt	2515:2518	arg1	Expt					2515:2518	Expt 2	2515:2520	Expt 2	2515:2520	Compared with controls, the YBG group of Expt 2 showed higher frequencies of Tregs (by 32%; P = 0.043) in the gut mucosa.
31879786	15	147	theme	score	2914:2918	arg1	loss					2848:2851	loss	2848:2851	loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04)	2848:2937	Compared with the YBG group, the YBG-(Abx) group of Expt 5 showed aggravated colitis features including loss of body weight (by >100%; P < 0.01) and colonic inflammation score (by 42%; P = 0.04).
31879786	12	148	dep	%	2453:2453	arg1	P = 0.013					2456:2464	P = 0.013	2456:2464	57%; P = 0.013	2451:2464	The YBG group also showed significantly higher concentrations of fecal SCFAs such as acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids.
31879786	9	149	theme	colitis	1727:1733	arg1	severity					1735:1742	colitis severity	1727:1742	colitis severity	1727:1742	Expt 5: female mice were treated as in Expt 1, given drinking water with or without antibiotics during days 16-40, and colitis severity and intestinal cytokine production were determined.
31879786	16	150	theme	B6	2966:2967	arg1	mice					2969:2972	B6 mice	2966:2972	B6 mice	2966:2972	CONCLUSIONS Studies using B6 mice show that dietary BGs are beneficial for promoting intestinal health when the gut microbiota is intact.
31879786	0	151	theme	Complex	32:38	arg1	Polysaccharides					48:62	Yeast-Derived Complex Dietary Polysaccharides	18:62	Yeast-Derived Complex Dietary Polysaccharides	18:62	Pretreatment with Yeast-Derived Complex Dietary Polysaccharides Suppresses Gut Inflammation, Alters the Microbiota Composition, and Increases Immune Regulatory Short-Chain Fatty Acid Production in C57BL/6 Mice.
31879786	14	152	theme	gut	2609:2611	arg1	microbiota					2613:2622	gut microbiota	2609:2622	gut microbiota	2609:2622	Depletion of gut microbiota in the YBG group of mice caused diminished fecal SCFA concentrations (Expt 3) and intestinal Treg frequencies (Expt 4).
31879786	11	153	dep	%	2276:2276	arg1	P < 0.001					2279:2287	P < 0.001	2279:2287	66%; P < 0.001	2274:2287	The YBG group of Expt 2 showed a shift in the abundance of gut microbiota towards Bacteroides (by 16%; P = 0.049) and Verrucomicrobia (mean ± SD: control = 7.8 ± 0.44 vs. YBG = 21.0 ± 9.6%) and a reduction in Firmicutes (by 66%; P < 0.001).
31879786	12	154	theme	fecal	2356:2360	arg1	SCFAs					2362:2366	fecal SCFAs	2356:2366	fecal SCFAs	2356:2366	The YBG group also showed significantly higher concentrations of fecal SCFAs such as acetic (by 37%; P = 0.016), propionic (by 47%; P = 0.026), and butyric (by 57%; P = 0.013) acids.
31879786	3	155	theme	study	460:464	arg1	aim					448:450	The aim	444:450	The aim of this study	444:464	OBJECTIVES The aim of this study was to investigate the impact of orally administering highly purified, yeast-derived BG (YBG; β-1,3/1,6-d-glucan) on susceptibility to colitis.
31879786	9	156	theme	cytokine	1759:1766	arg1	production					1768:1777	intestinal cytokine production	1748:1777	intestinal cytokine production	1748:1777	Expt 5: female mice were treated as in Expt 1, given drinking water with or without antibiotics during days 16-40, and colitis severity and intestinal cytokine production were determined.
31879786	2	157	theme	gut	395:397	arg1	inflammation					399:410	gut inflammation	395:410	gut inflammation	395:410	However, the effects of orally administered highly purified BGs on gut inflammation are largely unknown.
31879786	0	158	theme	Gut	75:77	arg1	Inflammation					79:90	Gut Inflammation	75:90	Gut Inflammation	75:90	Pretreatment with Yeast-Derived Complex Dietary Polysaccharides Suppresses Gut Inflammation, Alters the Microbiota Composition, and Increases Immune Regulatory Short-Chain Fatty Acid Production in C57BL/6 Mice.
31879786	11	159	dep	%	2150:2150	arg1	P = 0.049					2153:2161	P = 0.049	2153:2161	16%; P = 0.049	2148:2161	The YBG group of Expt 2 showed a shift in the abundance of gut microbiota towards Bacteroides (by 16%; P = 0.049) and Verrucomicrobia (mean ± SD: control = 7.8 ± 0.44 vs. YBG = 21.0 ± 9.6%) and a reduction in Firmicutes (by 66%; P < 0.001).
31879786	3	160	dep	OBJECTIVES	433:442	arg1	was					466:468	was	466:468	was to investigate the impact of orally administering highly purified, yeast-derived BG (YBG; β-1,3/1,6-d-glucan) on susceptibility to colitis	466:607	OBJECTIVES The aim of this study was to investigate the impact of orally administering highly purified, yeast-derived BG (YBG; β-1,3/1,6-d-glucan) on susceptibility to colitis.
31879786	6	161	theme	B6	979:980	arg1	mice					982:985	female B6 mice	972:985	female B6 mice	972:985	Expt 2: female B6 mice were treated with saline or YBG for 30 d and intestinal immune phenotype, gut microbiota composition, and fecal SCFA concentrations were determined.
31879786	11	162	theme	control = 7.8 ± 0.44 vs.	2196:2219	arg1	%					2237:2237	control = 7.8 ± 0.44 vs. YBG = 21.0 ± 9.6%	2196:2237	mean ± SD: control = 7.8 ± 0.44 vs. YBG = 21.0 ± 9.6%	2185:2237	The YBG group of Expt 2 showed a shift in the abundance of gut microbiota towards Bacteroides (by 16%; P = 0.049) and Verrucomicrobia (mean ± SD: control = 7.8 ± 0.44 vs. YBG = 21.0 ± 9.6%) and a reduction in Firmicutes (by 66%; P < 0.001).
31879786	9	163	dep	days	1711:1714	arg1	16-40					1716:1720	16-40	1716:1720	16-40	1716:1720	Expt 5: female mice were treated as in Expt 1, given drinking water with or without antibiotics during days 16-40, and colitis severity and intestinal cytokine production were determined.
31879786	14	164	theme	intestinal	2706:2715	arg1	Expt					2735:2738	Expt 4	2735:2740	Expt 4	2735:2740	Depletion of gut microbiota in the YBG group of mice caused diminished fecal SCFA concentrations (Expt 3) and intestinal Treg frequencies (Expt 4).
31879786	14	164	theme	intestinal	2706:2715	arg1	frequencies					2722:2732	intestinal Treg frequencies	2706:2732	intestinal Treg frequencies (Expt 4)	2706:2741	Depletion of gut microbiota in the YBG group of mice caused diminished fecal SCFA concentrations (Expt 3) and intestinal Treg frequencies (Expt 4).
31879786	7	165	dep	Expt	1136:1139	arg1	determined					1407:1416	determined	1407:1416	were determined	1402:1416	Expt 3: female B6 mice were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30, and gut immune phenotype and fecal SCFA concentrations were determined.
31879786	7	165	dep	Expt	1136:1139	arg1	treated					1164:1170	treated	1164:1170	were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30, and gut immune phenotype	1159:1370	Expt 3: female B6 mice were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16-30, and gut immune phenotype and fecal SCFA concentrations were determined.
31879786	10	166	from	group	1836:1840	arg1	Expt					1845:1848	Expt 1	1845:1850	Expt 1	1845:1850	RESULTS Compared with controls, the YBG group in Expt 1 exhibited suppressive effects on features of colitis, such as loss of body weight (by 47%; P < 0.001), shortening of colon (by 24%; P = 0.016), and histopathology severity score (by 45%; P = 0.01).
32630158	0	0	theme	Bio-Composites	81:94	arg1	Conductivity					50:61	Ionic Conductivity	44:61	Ionic Conductivity of Silk/Cellulose Bio-Composites Fabricated from Ionic Liquid and Varying Percentages of Coagulation Agents	44:169	The Impact of Composition and Morphology on Ionic Conductivity of Silk/Cellulose Bio-Composites Fabricated from Ionic Liquid and Varying Percentages of Coagulation Agents.
32630158	5	1	from	component	1320:1328	arg1	biocomposite					1337:1348	the biocomposite	1333:1348	the biocomposite	1333:1348	The results revealed that when silk is the dominating component in the biocomposite, the ionic conductivity is higher, which also correlates with higher β-sheet content.
32630158	4	2	theme	X-ray	1149:1153	arg1	scattering					1155:1164	X-ray scattering	1149:1164	X-ray scattering	1149:1164	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	0	3	theme	Silk/Cellulose	66:79	arg1	Bio-Composites					81:94	Silk/Cellulose Bio-Composites	66:94	Silk/Cellulose Bio-Composites Fabricated from Ionic Liquid and Varying Percentages of Coagulation Agents	66:169	The Impact of Composition and Morphology on Ionic Conductivity of Silk/Cellulose Bio-Composites Fabricated from Ionic Liquid and Varying Percentages of Coagulation Agents.
32630158	0	4	from	Impact	4:9	arg1	Conductivity					50:61	Ionic Conductivity	44:61	Ionic Conductivity of Silk/Cellulose Bio-Composites Fabricated from Ionic Liquid and Varying Percentages of Coagulation Agents	44:169	The Impact of Composition and Morphology on Ionic Conductivity of Silk/Cellulose Bio-Composites Fabricated from Ionic Liquid and Varying Percentages of Coagulation Agents.
32630158	4	5	theme	electron	997:1004	arg1	microscopy					1006:1015	scanning electron microscopy	988:1015	scanning electron microscopy (SEM)	988:1021	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	4	5	theme	electron	997:1004	arg1	SEM					1018:1020	SEM	1018:1020	SEM	1018:1020	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	1	6	theme	Blended	172:178	arg1	biocomposites					180:192	Blended biocomposites	172:192	Blended biocomposites created from the electrostatic and hydrophobic interactions between polysaccharides and structural proteins	172:300	Blended biocomposites created from the electrostatic and hydrophobic interactions between polysaccharides and structural proteins exhibit useful and unique properties.
32630158	4	7	theme	atomic	1167:1172	arg1	microscopy					1180:1189	atomic force microscopy	1167:1189	atomic force microscopy (AFM) based nanoindentation	1167:1217	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	4	7	theme	atomic	1167:1172	arg1	AFM					1192:1194	AFM	1192:1194	AFM	1192:1194	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	4	8	theme	bio-electrolyte	844:858	arg1	membranes					860:868	polysaccharide-protein bio-electrolyte membranes	821:868	polysaccharide-protein bio-electrolyte membranes	821:868	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	1	9	theme	useful	310:315	arg1	properties					328:337	useful and unique properties	310:337	useful and unique properties	310:337	Blended biocomposites created from the electrostatic and hydrophobic interactions between polysaccharides and structural proteins exhibit useful and unique properties.
32630158	4	10	theme	polysaccharide-protein	821:842	arg1	membranes					860:868	polysaccharide-protein bio-electrolyte membranes	821:868	polysaccharide-protein bio-electrolyte membranes	821:868	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	4	11	theme	dielectric	1224:1233	arg1	DRS					1260:1262	DRS	1260:1262	DRS	1260:1262	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	4	11	theme	dielectric	1224:1233	arg1	spectroscopy					1246:1257	dielectric relaxation spectroscopy	1224:1257	dielectric relaxation spectroscopy (DRS)	1224:1263	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	0	12	theme	Liquid	118:123	arg1	Percentages					137:147	Ionic Liquid and Varying Percentages	112:147	Ionic Liquid and Varying Percentages of Coagulation Agents	112:169	The Impact of Composition and Morphology on Ionic Conductivity of Silk/Cellulose Bio-Composites Fabricated from Ionic Liquid and Varying Percentages of Coagulation Agents.
32630158	5	13	theme	higher	1412:1417	arg1	content					1427:1433	higher β-sheet content	1412:1433	higher β-sheet content	1412:1433	The results revealed that when silk is the dominating component in the biocomposite, the ionic conductivity is higher, which also correlates with higher β-sheet content.
32630158	5	14	theme	β-sheet	1419:1425	arg1	content					1427:1433	higher β-sheet content	1412:1433	higher β-sheet content	1412:1433	The results revealed that when silk is the dominating component in the biocomposite, the ionic conductivity is higher, which also correlates with higher β-sheet content.
32630158	0	15	theme	Ionic	112:116	arg1	Percentages					137:147	Ionic Liquid and Varying Percentages	112:147	Ionic Liquid and Varying Percentages of Coagulation Agents	112:169	The Impact of Composition and Morphology on Ionic Conductivity of Silk/Cellulose Bio-Composites Fabricated from Ionic Liquid and Varying Percentages of Coagulation Agents.
32630158	4	16	theme	differential	1108:1119	arg1	DSC					1143:1145	DSC	1143:1145	DSC	1143:1145	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	4	16	theme	differential	1108:1119	arg1	calorimetry					1130:1140	differential scanning calorimetry	1108:1140	differential scanning calorimetry (DSC)	1108:1146	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	3	17	theme	numerous	580:587	arg1	properties					589:598	numerous properties	580:598	numerous properties of the Bombyx mori silk and microcrystalline cellulose biocomposites blended using ionic liquid and regenerated with various coagulation agents	580:742	In this particular study, numerous properties of the Bombyx mori silk and microcrystalline cellulose biocomposites blended using ionic liquid and regenerated with various coagulation agents were investigated.
32630158	4	18	theme	gravimetric	1080:1090	arg1	TGA					1102:1104	TGA	1102:1104	TGA	1102:1104	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	4	18	theme	gravimetric	1080:1090	arg1	analysis					1092:1099	thermal gravimetric analysis	1072:1099	thermal gravimetric analysis (TGA)	1072:1105	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	4	19	theme	scanning	988:995	arg1	microscopy					1006:1015	scanning electron microscopy	988:1015	scanning electron microscopy (SEM)	988:1021	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	4	19	theme	scanning	988:995	arg1	SEM					1018:1020	SEM	1018:1020	SEM	1018:1020	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	3	20	theme	mori	614:617	arg1	silk					619:622	Bombyx mori silk	607:622	Bombyx mori silk	607:622	In this particular study, numerous properties of the Bombyx mori silk and microcrystalline cellulose biocomposites blended using ionic liquid and regenerated with various coagulation agents were investigated.
32630158	4	21	theme	relaxation	1235:1244	arg1	DRS					1260:1262	DRS	1260:1262	DRS	1260:1262	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	4	21	theme	relaxation	1235:1244	arg1	spectroscopy					1246:1257	dielectric relaxation spectroscopy	1224:1257	dielectric relaxation spectroscopy (DRS)	1224:1263	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	6	22	from	component	1483:1491	arg1	biocomposite					1500:1511	the biocomposite	1496:1511	the biocomposite	1496:1511	However, when cellulose becomes the dominating component in the biocomposite, this relationship is not observed; instead, cellulose semicrystallinity and mechanical properties dominate the ionic conduction.
32630158	1	23	theme	unique	321:326	arg1	properties					328:337	useful and unique properties	310:337	useful and unique properties	310:337	Blended biocomposites created from the electrostatic and hydrophobic interactions between polysaccharides and structural proteins exhibit useful and unique properties.
32630158	3	24	theme	silk	619:622	arg1	biocomposites					655:667	the Bombyx mori silk and microcrystalline cellulose biocomposites	603:667	the Bombyx mori silk and microcrystalline cellulose biocomposites blended using ionic liquid and regenerated with various coagulation agents	603:742	In this particular study, numerous properties of the Bombyx mori silk and microcrystalline cellulose biocomposites blended using ionic liquid and regenerated with various coagulation agents were investigated.
32630158	6	25	theme	ionic	1625:1629	arg1	conduction					1631:1640	the ionic conduction	1621:1640	the ionic conduction	1621:1640	However, when cellulose becomes the dominating component in the biocomposite, this relationship is not observed; instead, cellulose semicrystallinity and mechanical properties dominate the ionic conduction.
32630158	3	26	theme	various	717:723	arg1	agents					737:742	various coagulation agents	717:742	various coagulation agents	717:742	In this particular study, numerous properties of the Bombyx mori silk and microcrystalline cellulose biocomposites blended using ionic liquid and regenerated with various coagulation agents were investigated.
32630158	0	27	theme	Composition	14:24	arg1	Impact					4:9	The Impact	0:9	The Impact of Composition and Morphology on Ionic Conductivity of Silk/Cellulose Bio-Composites Fabricated from Ionic Liquid and Varying Percentages of Coagulation Agents.	0:170	The Impact of Composition and Morphology on Ionic Conductivity of Silk/Cellulose Bio-Composites Fabricated from Ionic Liquid and Varying Percentages of Coagulation Agents.
32630158	0	28	theme	Varying	129:135	arg1	Percentages					137:147	Ionic Liquid and Varying Percentages	112:147	Ionic Liquid and Varying Percentages of Coagulation Agents	112:169	The Impact of Composition and Morphology on Ionic Conductivity of Silk/Cellulose Bio-Composites Fabricated from Ionic Liquid and Varying Percentages of Coagulation Agents.
32630158	4	29	theme	numerous	940:947	arg1	techniques					966:975	numerous characterization techniques	940:975	numerous characterization techniques	940:975	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	4	30	theme	membranes	860:868	arg1	conductivity					909:920	ionic conductivity	903:920	ionic conductivity	903:920	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	4	30	theme	membranes	860:868	arg1	morphology					888:897	the resulting morphology	874:897	the resulting morphology	874:897	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	4	30	theme	membranes	860:868	arg1	composition					806:816	the composition	802:816	the composition of polysaccharide-protein bio-electrolyte membranes	802:868	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	1	31	theme	electrostatic	211:223	arg1	interactions					241:252	the electrostatic and hydrophobic interactions	207:252	the electrostatic and hydrophobic interactions between polysaccharides and structural proteins	207:300	Blended biocomposites created from the electrostatic and hydrophobic interactions between polysaccharides and structural proteins exhibit useful and unique properties.
32630158	4	32	theme	infrared	1042:1049	arg1	spectroscopy					1051:1062	infrared spectroscopy	1042:1062	infrared spectroscopy (FTIR)	1042:1069	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	4	32	theme	infrared	1042:1049	arg1	FTIR					1065:1068	FTIR	1065:1068	FTIR	1065:1068	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	3	33	theme	Bombyx	607:612	arg1	mori					614:617	Bombyx mori	607:617	Bombyx mori silk	607:622	In this particular study, numerous properties of the Bombyx mori silk and microcrystalline cellulose biocomposites blended using ionic liquid and regenerated with various coagulation agents were investigated.
32630158	2	34	theme	properties	470:479	arg1	coupling					427:434	the coupling	423:434	the coupling of the material's physicochemical properties to its morphology	423:497	However, engineering these biopolymers into applicable forms is challenging due to the coupling of the material's physicochemical properties to its morphology, and the undertaking that comes with controlling this.
32630158	0	35	theme	Morphology	30:39	arg1	Impact					4:9	The Impact	0:9	The Impact of Composition and Morphology on Ionic Conductivity of Silk/Cellulose Bio-Composites Fabricated from Ionic Liquid and Varying Percentages of Coagulation Agents.	0:170	The Impact of Composition and Morphology on Ionic Conductivity of Silk/Cellulose Bio-Composites Fabricated from Ionic Liquid and Varying Percentages of Coagulation Agents.
32630158	1	36	theme	hydrophobic	229:239	arg1	interactions					241:252	the electrostatic and hydrophobic interactions	207:252	the electrostatic and hydrophobic interactions between polysaccharides and structural proteins	207:300	Blended biocomposites created from the electrostatic and hydrophobic interactions between polysaccharides and structural proteins exhibit useful and unique properties.
32630158	3	37	theme	cellulose	645:653	arg1	biocomposites					655:667	the Bombyx mori silk and microcrystalline cellulose biocomposites	603:667	the Bombyx mori silk and microcrystalline cellulose biocomposites blended using ionic liquid and regenerated with various coagulation agents	603:742	In this particular study, numerous properties of the Bombyx mori silk and microcrystalline cellulose biocomposites blended using ionic liquid and regenerated with various coagulation agents were investigated.
32630158	3	38	dep	biocomposites	655:667	arg1	regenerated					700:710	regenerated	700:710	regenerated with various coagulation agents	700:742	In this particular study, numerous properties of the Bombyx mori silk and microcrystalline cellulose biocomposites blended using ionic liquid and regenerated with various coagulation agents were investigated.
32630158	3	38	dep	biocomposites	655:667	arg1	blended					669:675	blended	669:675	blended using ionic liquid	669:694	In this particular study, numerous properties of the Bombyx mori silk and microcrystalline cellulose biocomposites blended using ionic liquid and regenerated with various coagulation agents were investigated.
32630158	2	39	theme	physicochemical	454:468	arg1	properties					470:479	the material's physicochemical properties	439:479	the material's physicochemical properties	439:479	However, engineering these biopolymers into applicable forms is challenging due to the coupling of the material's physicochemical properties to its morphology, and the undertaking that comes with controlling this.
32630158	3	40	theme	biocomposites	655:667	arg1	properties					589:598	numerous properties	580:598	numerous properties of the Bombyx mori silk and microcrystalline cellulose biocomposites blended using ionic liquid and regenerated with various coagulation agents	580:742	In this particular study, numerous properties of the Bombyx mori silk and microcrystalline cellulose biocomposites blended using ionic liquid and regenerated with various coagulation agents were investigated.
32630158	4	41	theme	thermal	1072:1078	arg1	TGA					1102:1104	TGA	1102:1104	TGA	1102:1104	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	4	41	theme	thermal	1072:1078	arg1	analysis					1092:1099	thermal gravimetric analysis	1072:1099	thermal gravimetric analysis (TGA)	1072:1105	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	0	42	theme	Ionic	44:48	arg1	Conductivity					50:61	Ionic Conductivity	44:61	Ionic Conductivity of Silk/Cellulose Bio-Composites Fabricated from Ionic Liquid and Varying Percentages of Coagulation Agents	44:169	The Impact of Composition and Morphology on Ionic Conductivity of Silk/Cellulose Bio-Composites Fabricated from Ionic Liquid and Varying Percentages of Coagulation Agents.
32630158	0	43	theme	Agents	164:169	arg1	Percentages					137:147	Ionic Liquid and Varying Percentages	112:147	Ionic Liquid and Varying Percentages of Coagulation Agents	112:169	The Impact of Composition and Morphology on Ionic Conductivity of Silk/Cellulose Bio-Composites Fabricated from Ionic Liquid and Varying Percentages of Coagulation Agents.
32630158	4	44	theme	characterization	949:964	arg1	techniques					966:975	numerous characterization techniques	940:975	numerous characterization techniques	940:975	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	4	45	theme	scanning	1121:1128	arg1	DSC					1143:1145	DSC	1143:1145	DSC	1143:1145	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	4	45	theme	scanning	1121:1128	arg1	calorimetry					1130:1140	differential scanning calorimetry	1108:1140	differential scanning calorimetry (DSC)	1108:1146	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	0	46	theme	Coagulation	152:162	arg1	Agents					164:169	Coagulation Agents	152:169	Coagulation Agents	152:169	The Impact of Composition and Morphology on Ionic Conductivity of Silk/Cellulose Bio-Composites Fabricated from Ionic Liquid and Varying Percentages of Coagulation Agents.
32630158	3	47	theme	microcrystalline	628:643	arg1	cellulose					645:653	microcrystalline cellulose	628:653	microcrystalline cellulose	628:653	In this particular study, numerous properties of the Bombyx mori silk and microcrystalline cellulose biocomposites blended using ionic liquid and regenerated with various coagulation agents were investigated.
32630158	6	48	theme	mechanical	1590:1599	arg1	properties					1601:1610	mechanical properties	1590:1610	mechanical properties	1590:1610	However, when cellulose becomes the dominating component in the biocomposite, this relationship is not observed; instead, cellulose semicrystallinity and mechanical properties dominate the ionic conduction.
32630158	4	49	theme	ionic	903:907	arg1	conductivity					909:920	ionic conductivity	903:920	ionic conductivity	903:920	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	2	50	theme	applicable	384:393	arg1	forms					395:399	applicable forms	384:399	applicable forms	384:399	However, engineering these biopolymers into applicable forms is challenging due to the coupling of the material's physicochemical properties to its morphology, and the undertaking that comes with controlling this.
32630158	4	51	theme	force	1174:1178	arg1	microscopy					1180:1189	atomic force microscopy	1167:1189	atomic force microscopy (AFM) based nanoindentation	1167:1217	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	4	51	theme	force	1174:1178	arg1	AFM					1192:1194	AFM	1192:1194	AFM	1192:1194	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	5	52	theme	dominating	1309:1318	arg1	component					1320:1328	the dominating component	1305:1328	the dominating component in the biocomposite	1305:1348	The results revealed that when silk is the dominating component in the biocomposite, the ionic conductivity is higher, which also correlates with higher β-sheet content.
32630158	5	52	theme	dominating	1309:1318	arg1	silk					1297:1300	silk	1297:1300	silk	1297:1300	The results revealed that when silk is the dominating component in the biocomposite, the ionic conductivity is higher, which also correlates with higher β-sheet content.
32630158	4	53	theme	resulting	878:886	arg1	morphology					888:897	the resulting morphology	874:897	the resulting morphology	874:897	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	3	54	theme	coagulation	725:735	arg1	agents					737:742	various coagulation agents	717:742	various coagulation agents	717:742	In this particular study, numerous properties of the Bombyx mori silk and microcrystalline cellulose biocomposites blended using ionic liquid and regenerated with various coagulation agents were investigated.
32630158	5	55	theme	ionic	1355:1359	arg1	higher					1377:1382	higher	1377:1382	higher	1377:1382	The results revealed that when silk is the dominating component in the biocomposite, the ionic conductivity is higher, which also correlates with higher β-sheet content.
32630158	5	55	theme	ionic	1355:1359	arg1	conductivity					1361:1372	the ionic conductivity	1351:1372	the ionic conductivity	1351:1372	The results revealed that when silk is the dominating component in the biocomposite, the ionic conductivity is higher, which also correlates with higher β-sheet content.
32630158	3	56	theme	particular	562:571	arg1	study					573:577	this particular study	557:577	this particular study	557:577	In this particular study, numerous properties of the Bombyx mori silk and microcrystalline cellulose biocomposites blended using ionic liquid and regenerated with various coagulation agents were investigated.
32630158	6	57	theme	cellulose	1558:1566	arg1	semicrystallinity					1568:1584	cellulose semicrystallinity	1558:1584	cellulose semicrystallinity	1558:1584	However, when cellulose becomes the dominating component in the biocomposite, this relationship is not observed; instead, cellulose semicrystallinity and mechanical properties dominate the ionic conduction.
32630158	4	58	dep	using	934:938	arg1	transform					1032:1040	transform	1032:1040	transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS)	1032:1263	Specifically, the relationship between the composition of polysaccharide-protein bio-electrolyte membranes and the resulting morphology and ionic conductivity is explored using numerous characterization techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, atomic force microscopy (AFM) based nanoindentation, and dielectric relaxation spectroscopy (DRS).
32630158	6	59	theme	dominating	1472:1481	arg1	component					1483:1491	the dominating component	1468:1491	the dominating component in the biocomposite	1468:1511	However, when cellulose becomes the dominating component in the biocomposite, this relationship is not observed; instead, cellulose semicrystallinity and mechanical properties dominate the ionic conduction.
32630158	1	60	theme	structural	282:291	arg1	proteins					293:300	structural proteins	282:300	structural proteins	282:300	Blended biocomposites created from the electrostatic and hydrophobic interactions between polysaccharides and structural proteins exhibit useful and unique properties.
33877829	0	0	theme	Disaccharides	82:94	arg1	Library					27:33	an Exhaustive Library	13:33	an Exhaustive Library of Naturally Occurring Galf-Manp and Galp-Manp Disaccharides	13:94	Synthesis of an Exhaustive Library of Naturally Occurring Galf-Manp and Galp-Manp Disaccharides.
33877829	3	1	theme	hexosides	323:331	arg1	size					315:318	the ring size	306:318	the ring size of hexosides	306:331	Among numerous structural modulations, the nature of the ring size of hexosides may induce significant differences on both biological and physicochemical properties of the glycoconjugate of interest.
33877829	5	2	theme	ion	751:753	arg1	IM					765:766	IM	765:766	IM	765:766	On the basis of the scope of mass spectrometry, two analytical techniques to register those signatures were considered, i.e., the ion mobility (IM) and the infrared multiple photon dissociation (IRMPD), in order to build up cross-linked databases.
33877829	5	2	theme	ion	751:753	arg1	mobility					755:762	the ion mobility	747:762	the ion mobility (IM)	747:767	On the basis of the scope of mass spectrometry, two analytical techniques to register those signatures were considered, i.e., the ion mobility (IM) and the infrared multiple photon dissociation (IRMPD), in order to build up cross-linked databases.
33877829	1	3	theme	Spectrometry-Based	159:176	arg1	IM-MS					178:182	Advanced Mass Spectrometry-Based IM-MS	145:182	Advanced Mass Spectrometry-Based IM-MS	145:182	Toward Fingerprinting According to Ring Size by Advanced Mass Spectrometry-Based IM-MS and IRMPD.
33877829	0	4	theme	Galp-Manp	72:80	arg1	Disaccharides					82:94	Naturally Occurring Galf-Manp and Galp-Manp Disaccharides	38:94	Naturally Occurring Galf-Manp and Galp-Manp Disaccharides	38:94	Synthesis of an Exhaustive Library of Naturally Occurring Galf-Manp and Galp-Manp Disaccharides.
33877829	5	5	theme	mass	650:653	arg1	spectrometry					655:666	mass spectrometry	650:666	mass spectrometry	650:666	On the basis of the scope of mass spectrometry, two analytical techniques to register those signatures were considered, i.e., the ion mobility (IM) and the infrared multiple photon dissociation (IRMPD), in order to build up cross-linked databases.
33877829	8	6	theme	glycosyl	1315:1322	arg1	entities					1324:1331	the two glycosyl entities	1307:1331	the two glycosyl entities	1307:1331	Both techniques used afforded signatures, specific to the nature of the connectivity between the two glycosyl entities.
33877829	6	7	theme	possible	950:957	arg1	regioisomers					959:970	all possible regioisomers	946:970	all possible regioisomers	946:970	d-Galactose occurs in natural products in both tautomeric forms and presents all possible regioisomers when linked to d-mannose.
33877829	3	8	theme	biological	376:385	arg1	properties					407:416	both biological and physicochemical properties	371:416	both biological and physicochemical properties of the glycoconjugate of interest	371:450	Among numerous structural modulations, the nature of the ring size of hexosides may induce significant differences on both biological and physicochemical properties of the glycoconjugate of interest.
33877829	5	9	theme	spectrometry	655:666	arg1	scope					641:645	the scope	637:645	the scope of mass spectrometry	637:666	On the basis of the scope of mass spectrometry, two analytical techniques to register those signatures were considered, i.e., the ion mobility (IM) and the infrared multiple photon dissociation (IRMPD), in order to build up cross-linked databases.
33877829	5	10	theme	cross-linked	845:856	arg1	databases					858:866	cross-linked databases	845:866	cross-linked databases	845:866	On the basis of the scope of mass spectrometry, two analytical techniques to register those signatures were considered, i.e., the ion mobility (IM) and the infrared multiple photon dissociation (IRMPD), in order to build up cross-linked databases.
33877829	4	11	theme	small	488:492	arg1	disaccharides					494:506	small disaccharides	488:506	small disaccharides bearing either a furanosyl entity or a pyranosyl residue	488:563	On this assumption, we expect that small disaccharides bearing either a furanosyl entity or a pyranosyl residue would give a specific signature, even in the gas phase.
33877829	3	12	theme	structural	268:277	arg1	modulations					279:289	numerous structural modulations	259:289	numerous structural modulations	259:289	Among numerous structural modulations, the nature of the ring size of hexosides may induce significant differences on both biological and physicochemical properties of the glycoconjugate of interest.
33877829	7	13	theme	IM-MS	1167:1171	arg1	data					1186:1189	IM-MS and IRMPD-MS data	1167:1189	IM-MS and IRMPD-MS data	1167:1189	Consequently, the four reducing Galf-Manp disaccharides as well as the four Galp-Manp counterparts were first synthesized according to a highly convergent approach, and IM-MS and IRMPD-MS data were second collected.
33877829	6	14	theme	tautomeric	916:925	arg1	forms					927:931	both tautomeric forms	911:931	both tautomeric forms	911:931	d-Galactose occurs in natural products in both tautomeric forms and presents all possible regioisomers when linked to d-mannose.
33877829	5	15	theme	infrared	777:784	arg1	IRMPD					816:820	IRMPD	816:820	IRMPD	816:820	On the basis of the scope of mass spectrometry, two analytical techniques to register those signatures were considered, i.e., the ion mobility (IM) and the infrared multiple photon dissociation (IRMPD), in order to build up cross-linked databases.
33877829	5	15	theme	infrared	777:784	arg1	dissociation					802:813	the infrared multiple photon dissociation	773:813	the infrared multiple photon dissociation (IRMPD)	773:821	On the basis of the scope of mass spectrometry, two analytical techniques to register those signatures were considered, i.e., the ion mobility (IM) and the infrared multiple photon dissociation (IRMPD), in order to build up cross-linked databases.
33877829	3	16	theme	numerous	259:266	arg1	modulations					279:289	numerous structural modulations	259:289	numerous structural modulations	259:289	Among numerous structural modulations, the nature of the ring size of hexosides may induce significant differences on both biological and physicochemical properties of the glycoconjugate of interest.
33877829	0	17	theme	Exhaustive	16:25	arg1	Library					27:33	an Exhaustive Library	13:33	an Exhaustive Library of Naturally Occurring Galf-Manp and Galp-Manp Disaccharides	13:94	Synthesis of an Exhaustive Library of Naturally Occurring Galf-Manp and Galp-Manp Disaccharides.
33877829	5	18	link	cross-linked	845:856	arg1	databases					858:866	cross-linked databases	845:866	cross-linked databases	845:866	On the basis of the scope of mass spectrometry, two analytical techniques to register those signatures were considered, i.e., the ion mobility (IM) and the infrared multiple photon dissociation (IRMPD), in order to build up cross-linked databases.
33877829	3	19	theme	physicochemical	391:405	arg1	properties					407:416	both biological and physicochemical properties	371:416	both biological and physicochemical properties of the glycoconjugate of interest	371:450	Among numerous structural modulations, the nature of the ring size of hexosides may induce significant differences on both biological and physicochemical properties of the glycoconjugate of interest.
33877829	5	20	dep	mobility	755:762	arg1	i.e.					741:744	i.e.	741:744	i.e.	741:744	On the basis of the scope of mass spectrometry, two analytical techniques to register those signatures were considered, i.e., the ion mobility (IM) and the infrared multiple photon dissociation (IRMPD), in order to build up cross-linked databases.
33877829	4	21	theme	specific	578:585	arg1	signature					587:595	a specific signature	576:595	a specific signature	576:595	On this assumption, we expect that small disaccharides bearing either a furanosyl entity or a pyranosyl residue would give a specific signature, even in the gas phase.
33877829	7	22	theme	reducing	1021:1028	arg1	disaccharides					1040:1052	the four reducing Galf-Manp disaccharides	1012:1052	the four reducing Galf-Manp disaccharides as well as the four Galp-Manp counterparts	1012:1095	Consequently, the four reducing Galf-Manp disaccharides as well as the four Galp-Manp counterparts were first synthesized according to a highly convergent approach, and IM-MS and IRMPD-MS data were second collected.
33877829	8	23	theme	specific	1256:1263	arg1	signatures					1244:1253	signatures	1244:1253	signatures	1244:1253	Both techniques used afforded signatures, specific to the nature of the connectivity between the two glycosyl entities.
33877829	0	24	theme	Library	27:33	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of an Exhaustive Library of Naturally Occurring Galf-Manp and Galp-Manp Disaccharides.	0:95	Synthesis of an Exhaustive Library of Naturally Occurring Galf-Manp and Galp-Manp Disaccharides.
33877829	4	25	theme	pyranosyl	547:555	arg1	residue					557:563	a pyranosyl residue	545:563	a pyranosyl residue	545:563	On this assumption, we expect that small disaccharides bearing either a furanosyl entity or a pyranosyl residue would give a specific signature, even in the gas phase.
33877829	3	26	theme	interest	443:450	arg1	glycoconjugate					425:438	the glycoconjugate	421:438	the glycoconjugate of interest	421:450	Among numerous structural modulations, the nature of the ring size of hexosides may induce significant differences on both biological and physicochemical properties of the glycoconjugate of interest.
33877829	2	27	theme	derivatives	240:250	arg1	diversity					216:224	a huge diversity	209:224	a huge diversity of glycosidic derivatives	209:250	Nature offers a huge diversity of glycosidic derivatives.
33877829	5	28	theme	multiple	786:793	arg1	IRMPD					816:820	IRMPD	816:820	IRMPD	816:820	On the basis of the scope of mass spectrometry, two analytical techniques to register those signatures were considered, i.e., the ion mobility (IM) and the infrared multiple photon dissociation (IRMPD), in order to build up cross-linked databases.
33877829	5	28	theme	multiple	786:793	arg1	dissociation					802:813	the infrared multiple photon dissociation	773:813	the infrared multiple photon dissociation (IRMPD)	773:821	On the basis of the scope of mass spectrometry, two analytical techniques to register those signatures were considered, i.e., the ion mobility (IM) and the infrared multiple photon dissociation (IRMPD), in order to build up cross-linked databases.
33877829	0	29	theme	Occurring	48:56	arg1	Disaccharides					82:94	Naturally Occurring Galf-Manp and Galp-Manp Disaccharides	38:94	Naturally Occurring Galf-Manp and Galp-Manp Disaccharides	38:94	Synthesis of an Exhaustive Library of Naturally Occurring Galf-Manp and Galp-Manp Disaccharides.
33877829	3	30	theme	size	315:318	arg1	nature					296:301	the nature	292:301	the nature of the ring size of hexosides	292:331	Among numerous structural modulations, the nature of the ring size of hexosides may induce significant differences on both biological and physicochemical properties of the glycoconjugate of interest.
33877829	0	31	theme	Galf-Manp	58:66	arg1	Disaccharides					82:94	Naturally Occurring Galf-Manp and Galp-Manp Disaccharides	38:94	Naturally Occurring Galf-Manp and Galp-Manp Disaccharides	38:94	Synthesis of an Exhaustive Library of Naturally Occurring Galf-Manp and Galp-Manp Disaccharides.
33877829	2	32	theme	glycosidic	229:238	arg1	derivatives					240:250	glycosidic derivatives	229:250	glycosidic derivatives	229:250	Nature offers a huge diversity of glycosidic derivatives.
33877829	7	33	theme	Galf-Manp	1030:1038	arg1	disaccharides					1040:1052	the four reducing Galf-Manp disaccharides	1012:1052	the four reducing Galf-Manp disaccharides as well as the four Galp-Manp counterparts	1012:1095	Consequently, the four reducing Galf-Manp disaccharides as well as the four Galp-Manp counterparts were first synthesized according to a highly convergent approach, and IM-MS and IRMPD-MS data were second collected.
33877829	3	34	theme	glycoconjugate	425:438	arg1	properties					407:416	both biological and physicochemical properties	371:416	both biological and physicochemical properties of the glycoconjugate of interest	371:450	Among numerous structural modulations, the nature of the ring size of hexosides may induce significant differences on both biological and physicochemical properties of the glycoconjugate of interest.
33877829	7	35	theme	IRMPD-MS	1177:1184	arg1	data					1186:1189	IM-MS and IRMPD-MS data	1167:1189	IM-MS and IRMPD-MS data	1167:1189	Consequently, the four reducing Galf-Manp disaccharides as well as the four Galp-Manp counterparts were first synthesized according to a highly convergent approach, and IM-MS and IRMPD-MS data were second collected.
33877829	1	36	theme	Ring	132:135	arg1	Size					137:140	Ring Size	132:140	Ring Size by Advanced Mass Spectrometry-Based IM-MS and IRMPD	132:192	Toward Fingerprinting According to Ring Size by Advanced Mass Spectrometry-Based IM-MS and IRMPD.
33877829	6	37	theme	natural	891:897	arg1	products					899:906	natural products	891:906	natural products	891:906	d-Galactose occurs in natural products in both tautomeric forms and presents all possible regioisomers when linked to d-mannose.
33877829	5	38	theme	analytical	673:682	arg1	techniques					684:693	two analytical techniques	669:693	two analytical techniques to register those signatures	669:722	On the basis of the scope of mass spectrometry, two analytical techniques to register those signatures were considered, i.e., the ion mobility (IM) and the infrared multiple photon dissociation (IRMPD), in order to build up cross-linked databases.
33877829	2	39	theme	huge	211:214	arg1	diversity					216:224	a huge diversity	209:224	a huge diversity of glycosidic derivatives	209:250	Nature offers a huge diversity of glycosidic derivatives.
33877829	5	40	dep	scope	641:645	arg1	the					624:626	the	624:626	the	624:626	On the basis of the scope of mass spectrometry, two analytical techniques to register those signatures were considered, i.e., the ion mobility (IM) and the infrared multiple photon dissociation (IRMPD), in order to build up cross-linked databases.
33877829	5	40	dep	scope	641:645	arg1	basis					628:632	basis	628:632	basis	628:632	On the basis of the scope of mass spectrometry, two analytical techniques to register those signatures were considered, i.e., the ion mobility (IM) and the infrared multiple photon dissociation (IRMPD), in order to build up cross-linked databases.
33877829	3	41	theme	ring	310:313	arg1	size					315:318	the ring size	306:318	the ring size of hexosides	306:331	Among numerous structural modulations, the nature of the ring size of hexosides may induce significant differences on both biological and physicochemical properties of the glycoconjugate of interest.
33877829	7	42	theme	Galp-Manp	1074:1082	arg1	counterparts					1084:1095	the four Galp-Manp counterparts	1065:1095	the four reducing Galf-Manp disaccharides as well as the four Galp-Manp counterparts	1012:1095	Consequently, the four reducing Galf-Manp disaccharides as well as the four Galp-Manp counterparts were first synthesized according to a highly convergent approach, and IM-MS and IRMPD-MS data were second collected.
33877829	4	43	theme	gas	610:612	arg1	phase					614:618	the gas phase	606:618	the gas phase	606:618	On this assumption, we expect that small disaccharides bearing either a furanosyl entity or a pyranosyl residue would give a specific signature, even in the gas phase.
33877829	8	44	theme	connectivity	1286:1297	arg1	nature					1272:1277	the nature	1268:1277	the nature of the connectivity between the two glycosyl entities	1268:1331	Both techniques used afforded signatures, specific to the nature of the connectivity between the two glycosyl entities.
33877829	3	45	theme	significant	344:354	arg1	differences					356:366	significant differences	344:366	significant differences	344:366	Among numerous structural modulations, the nature of the ring size of hexosides may induce significant differences on both biological and physicochemical properties of the glycoconjugate of interest.
33877829	4	46	theme	furanosyl	525:533	arg1	entity					535:540	a furanosyl entity	523:540	a furanosyl entity	523:540	On this assumption, we expect that small disaccharides bearing either a furanosyl entity or a pyranosyl residue would give a specific signature, even in the gas phase.
33877829	5	47	theme	photon	795:800	arg1	IRMPD					816:820	IRMPD	816:820	IRMPD	816:820	On the basis of the scope of mass spectrometry, two analytical techniques to register those signatures were considered, i.e., the ion mobility (IM) and the infrared multiple photon dissociation (IRMPD), in order to build up cross-linked databases.
33877829	5	47	theme	photon	795:800	arg1	dissociation					802:813	the infrared multiple photon dissociation	773:813	the infrared multiple photon dissociation (IRMPD)	773:821	On the basis of the scope of mass spectrometry, two analytical techniques to register those signatures were considered, i.e., the ion mobility (IM) and the infrared multiple photon dissociation (IRMPD), in order to build up cross-linked databases.
33877829	7	48	theme	convergent	1142:1151	arg1	approach					1153:1160	a highly convergent approach	1133:1160	a highly convergent approach	1133:1160	Consequently, the four reducing Galf-Manp disaccharides as well as the four Galp-Manp counterparts were first synthesized according to a highly convergent approach, and IM-MS and IRMPD-MS data were second collected.
33877829	5	49	dep	techniques	684:693	arg1	register					698:705	register	698:705	to register those signatures	695:722	On the basis of the scope of mass spectrometry, two analytical techniques to register those signatures were considered, i.e., the ion mobility (IM) and the infrared multiple photon dissociation (IRMPD), in order to build up cross-linked databases.
34432331	7	0	dep	domains	1179:1185	arg1	endowed					1187:1193	endowed	1187:1193	endowed	1187:1193	Their structure allows the integration of domains endowed with biological functionality, making them attractive compounds to prepare composites with smart properties.
34432331	6	1	dep	polypeptides	1080:1091	arg1	HELPs					1094:1098	HELPs	1094:1098	HELPs	1094:1098	Human elastin-like polypeptides (HELPs) are stimuli-responsive biopolymers.
34432331	7	2	theme	attractive	1238:1247	arg1	compounds					1249:1257	attractive compounds	1238:1257	attractive compounds	1238:1257	Their structure allows the integration of domains endowed with biological functionality, making them attractive compounds to prepare composites with smart properties.
34432331	2	3	theme	restored	417:424	arg1	tissue					426:431	the restored tissue	413:431	the restored tissue	413:431	Three-dimensional (3D) advanced additive manufacturing technologies allow creating scaffolds with controlled and reproducible macro- and micro-structure that improve the quality of the restored tissue to favor spontaneous repair.
34432331	10	4	theme	bioactive	1651:1659	arg1	composites					1661:1670	these smart bioactive composites	1639:1670	these smart bioactive composites	1639:1670	Different constructs were prepared and the stimuli-responsive behavior as well as the biological activity were evaluated, suggesting that these smart bioactive composites are suitable to realize multifunctional dressings that sustain the local release of therapeutic adjuncts.
34432331	5	5	dep	bioactive	1041:1049	arg1	adjuncts					1051:1058	adjuncts	1051:1058	adjuncts	1051:1058	Precisely designed polysaccharide scaffolds obtained by 3D printing represent a robust platform that can be further implemented with the controlled delivery of bioactive adjuncts.
34432331	1	6	theme	skin	191:194	arg1	regeneration					203:214	skin tissue regeneration	191:214	skin tissue regeneration	191:214	Polysaccharide scaffolds have been successfully employed to reconstruct environments that sustain skin tissue regeneration after injuries.
34432331	10	7	dep	therapeutic	1756:1766	arg1	adjuncts					1768:1775	adjuncts	1768:1775	adjuncts	1768:1775	Different constructs were prepared and the stimuli-responsive behavior as well as the biological activity were evaluated, suggesting that these smart bioactive composites are suitable to realize multifunctional dressings that sustain the local release of therapeutic adjuncts.
34432331	7	8	theme	smart	1286:1290	arg1	properties					1292:1301	smart properties	1286:1301	smart properties	1286:1301	Their structure allows the integration of domains endowed with biological functionality, making them attractive compounds to prepare composites with smart properties.
34432331	10	9	theme	Different	1501:1509	arg1	constructs					1511:1520	Different constructs	1501:1520	Different constructs	1501:1520	Different constructs were prepared and the stimuli-responsive behavior as well as the biological activity were evaluated, suggesting that these smart bioactive composites are suitable to realize multifunctional dressings that sustain the local release of therapeutic adjuncts.
34432331	2	10	theme	reproducible	345:356	arg1	macro-					358:363	macro-	358:363	macro-	358:363	Three-dimensional (3D) advanced additive manufacturing technologies allow creating scaffolds with controlled and reproducible macro- and micro-structure that improve the quality of the restored tissue to favor spontaneous repair.
34432331	5	11	theme	bioactive	1041:1049	arg1	delivery					1029:1036	the controlled delivery	1014:1036	the controlled delivery of bioactive adjuncts	1014:1058	Precisely designed polysaccharide scaffolds obtained by 3D printing represent a robust platform that can be further implemented with the controlled delivery of bioactive adjuncts.
34432331	5	12	theme	controlled	1018:1027	arg1	delivery					1029:1036	the controlled delivery	1014:1036	the controlled delivery of bioactive adjuncts	1014:1058	Precisely designed polysaccharide scaffolds obtained by 3D printing represent a robust platform that can be further implemented with the controlled delivery of bioactive adjuncts.
34432331	10	13	theme	stimuli-responsive	1544:1561	arg1	behavior					1563:1570	the stimuli-responsive behavior	1540:1570	the stimuli-responsive behavior as well as the biological activity	1540:1605	Different constructs were prepared and the stimuli-responsive behavior as well as the biological activity were evaluated, suggesting that these smart bioactive composites are suitable to realize multifunctional dressings that sustain the local release of therapeutic adjuncts.
34432331	8	14	dep	components	1397:1406	arg1	HELP					1392:1395	HELP	1392:1395	HELP	1392:1395	In the present study, 3D-printed alginate and chitosan scaffolds were combined with the HELP components.
34432331	4	15	theme	standard	774:781	arg1	treatments					783:792	the standard treatments	770:792	the standard treatments	770:792	In these circumstances, the bioavailability of therapeutic adjuncts like the growth factors in addition to the standard treatments represents undoubtedly a promising strategy to accelerate the healing of skin lesions.
34432331	10	16	theme	multifunctional	1696:1710	arg1	dressings					1712:1720	multifunctional dressings	1696:1720	multifunctional dressings that sustain the local release of therapeutic adjuncts	1696:1775	Different constructs were prepared and the stimuli-responsive behavior as well as the biological activity were evaluated, suggesting that these smart bioactive composites are suitable to realize multifunctional dressings that sustain the local release of therapeutic adjuncts.
34432331	2	17	theme	controlled	330:339	arg1	macro-					358:363	macro-	358:363	macro-	358:363	Three-dimensional (3D) advanced additive manufacturing technologies allow creating scaffolds with controlled and reproducible macro- and micro-structure that improve the quality of the restored tissue to favor spontaneous repair.
34432331	1	18	theme	tissue	196:201	arg1	regeneration					203:214	skin tissue regeneration	191:214	skin tissue regeneration	191:214	Polysaccharide scaffolds have been successfully employed to reconstruct environments that sustain skin tissue regeneration after injuries.
34432331	8	19	theme	alginate	1337:1344	arg1	scaffolds					1359:1367	3D-printed alginate and chitosan scaffolds	1326:1367	3D-printed alginate and chitosan scaffolds	1326:1367	In the present study, 3D-printed alginate and chitosan scaffolds were combined with the HELP components.
34432331	10	20	theme	local	1739:1743	arg1	release					1745:1751	the local release	1735:1751	the local release of therapeutic adjuncts	1735:1775	Different constructs were prepared and the stimuli-responsive behavior as well as the biological activity were evaluated, suggesting that these smart bioactive composites are suitable to realize multifunctional dressings that sustain the local release of therapeutic adjuncts.
34432331	4	21	theme	therapeutic	710:720	arg1	bioavailability					691:705	the bioavailability	687:705	the bioavailability of therapeutic adjuncts like the growth factors in addition to the standard treatments	687:792	In these circumstances, the bioavailability of therapeutic adjuncts like the growth factors in addition to the standard treatments represents undoubtedly a promising strategy to accelerate the healing of skin lesions.
34432331	0	22	theme	scaffold	11:18	arg1	composites					20:29	3D-printed scaffold composites	0:29	3D-printed scaffold composites for the stimuli-induced local delivery of bioactive adjuncts	0:90	3D-printed scaffold composites for the stimuli-induced local delivery of bioactive adjuncts.
34432331	9	23	theme	epidermal	1446:1454	arg1	EGF					1471:1473	EGF	1471:1473	EGF	1471:1473	The HELP biopolymer was fused to the epidermal growth factor (EGF) as the bioactive domain.
34432331	9	23	theme	epidermal	1446:1454	arg1	factor					1463:1468	epidermal growth factor	1446:1468	the epidermal growth factor (EGF)	1442:1474	The HELP biopolymer was fused to the epidermal growth factor (EGF) as the bioactive domain.
34432331	9	24	theme	growth	1456:1461	arg1	EGF					1471:1473	EGF	1471:1473	EGF	1471:1473	The HELP biopolymer was fused to the epidermal growth factor (EGF) as the bioactive domain.
34432331	9	24	theme	growth	1456:1461	arg1	factor					1463:1468	epidermal growth factor	1446:1468	the epidermal growth factor (EGF)	1442:1474	The HELP biopolymer was fused to the epidermal growth factor (EGF) as the bioactive domain.
34432331	1	25	theme	Polysaccharide	93:106	arg1	scaffolds					108:116	Polysaccharide scaffolds	93:116	Polysaccharide scaffolds	93:116	Polysaccharide scaffolds have been successfully employed to reconstruct environments that sustain skin tissue regeneration after injuries.
34432331	2	26	theme	spontaneous	442:452	arg1	repair					454:459	spontaneous repair	442:459	spontaneous repair	442:459	Three-dimensional (3D) advanced additive manufacturing technologies allow creating scaffolds with controlled and reproducible macro- and micro-structure that improve the quality of the restored tissue to favor spontaneous repair.
34432331	0	27	theme	3D-printed	0:9	arg1	composites					20:29	3D-printed scaffold composites	0:29	3D-printed scaffold composites for the stimuli-induced local delivery of bioactive adjuncts	0:90	3D-printed scaffold composites for the stimuli-induced local delivery of bioactive adjuncts.
34432331	6	28	theme	elastin-like	1067:1078	arg1	polypeptides					1080:1091	Human elastin-like polypeptides	1061:1091	Human elastin-like polypeptides (HELPs)	1061:1099	Human elastin-like polypeptides (HELPs) are stimuli-responsive biopolymers.
34432331	6	28	theme	elastin-like	1067:1078	arg1	biopolymers					1124:1134	stimuli-responsive biopolymers	1105:1134	stimuli-responsive biopolymers	1105:1134	Human elastin-like polypeptides (HELPs) are stimuli-responsive biopolymers.
34432331	7	29	theme	biological	1200:1209	arg1	functionality					1211:1223	biological functionality	1200:1223	biological functionality	1200:1223	Their structure allows the integration of domains endowed with biological functionality, making them attractive compounds to prepare composites with smart properties.
34432331	3	30	theme	chronic	632:638	arg1	infection					640:648	chronic infection	632:648	chronic infection	632:648	However, when persistent inflammation occurs, the physiological tissue healing capacity is reduced, like in the presence of pathologies like diabetes, vascular diseases, chronic infection, and others.
34432331	5	31	theme	robust	961:966	arg1	platform					968:975	a robust platform	959:975	a robust platform that can be further implemented with the controlled delivery of bioactive adjuncts	959:1058	Precisely designed polysaccharide scaffolds obtained by 3D printing represent a robust platform that can be further implemented with the controlled delivery of bioactive adjuncts.
34432331	5	32	theme	designed	891:898	arg1	scaffolds					915:923	Precisely designed polysaccharide scaffolds	881:923	Precisely designed polysaccharide scaffolds obtained by 3D printing	881:947	Precisely designed polysaccharide scaffolds obtained by 3D printing represent a robust platform that can be further implemented with the controlled delivery of bioactive adjuncts.
34432331	6	33	theme	Human	1061:1065	arg1	polypeptides					1080:1091	Human elastin-like polypeptides	1061:1091	Human elastin-like polypeptides (HELPs)	1061:1099	Human elastin-like polypeptides (HELPs) are stimuli-responsive biopolymers.
34432331	6	33	theme	Human	1061:1065	arg1	biopolymers					1124:1134	stimuli-responsive biopolymers	1105:1134	stimuli-responsive biopolymers	1105:1134	Human elastin-like polypeptides (HELPs) are stimuli-responsive biopolymers.
34432331	7	34	theme	domains	1179:1185	arg1	integration					1164:1174	the integration	1160:1174	the integration of domains endowed with biological functionality	1160:1223	Their structure allows the integration of domains endowed with biological functionality, making them attractive compounds to prepare composites with smart properties.
34432331	0	35	dep	bioactive	73:81	arg1	adjuncts					83:90	adjuncts	83:90	adjuncts	83:90	3D-printed scaffold composites for the stimuli-induced local delivery of bioactive adjuncts.
34432331	9	36	theme	bioactive	1483:1491	arg1	biopolymer					1418:1427	biopolymer	1418:1427	The HELP biopolymer	1409:1427	The HELP biopolymer was fused to the epidermal growth factor (EGF) as the bioactive domain.
34432331	9	36	theme	bioactive	1483:1491	arg1	domain					1493:1498	the bioactive domain	1479:1498	the bioactive domain	1479:1498	The HELP biopolymer was fused to the epidermal growth factor (EGF) as the bioactive domain.
34432331	3	37	theme	healing	533:539	arg1	capacity					541:548	the physiological tissue healing capacity	508:548	the physiological tissue healing capacity	508:548	However, when persistent inflammation occurs, the physiological tissue healing capacity is reduced, like in the presence of pathologies like diabetes, vascular diseases, chronic infection, and others.
34432331	5	38	theme	polysaccharide	900:913	arg1	scaffolds					915:923	Precisely designed polysaccharide scaffolds	881:923	Precisely designed polysaccharide scaffolds obtained by 3D printing	881:947	Precisely designed polysaccharide scaffolds obtained by 3D printing represent a robust platform that can be further implemented with the controlled delivery of bioactive adjuncts.
34432331	3	39	theme	pathologies	586:596	arg1	presence					574:581	the presence	570:581	the presence of pathologies like diabetes, vascular diseases, chronic infection, and others	570:660	However, when persistent inflammation occurs, the physiological tissue healing capacity is reduced, like in the presence of pathologies like diabetes, vascular diseases, chronic infection, and others.
34432331	4	40	theme	promising	819:827	arg1	strategy					829:836	a promising strategy	817:836	a promising strategy to accelerate the healing of skin lesions	817:878	In these circumstances, the bioavailability of therapeutic adjuncts like the growth factors in addition to the standard treatments represents undoubtedly a promising strategy to accelerate the healing of skin lesions.
34432331	0	41	theme	stimuli-induced	39:53	arg1	delivery					61:68	the stimuli-induced local delivery	35:68	the stimuli-induced local delivery of bioactive adjuncts	35:90	3D-printed scaffold composites for the stimuli-induced local delivery of bioactive adjuncts.
34432331	2	42	theme	manufacturing	273:285	arg1	technologies					287:298	Three-dimensional (3D) advanced additive manufacturing technologies	232:298	Three-dimensional (3D) advanced additive manufacturing technologies	232:298	Three-dimensional (3D) advanced additive manufacturing technologies allow creating scaffolds with controlled and reproducible macro- and micro-structure that improve the quality of the restored tissue to favor spontaneous repair.
34432331	3	43	theme	physiological	512:524	arg1	capacity					541:548	the physiological tissue healing capacity	508:548	the physiological tissue healing capacity	508:548	However, when persistent inflammation occurs, the physiological tissue healing capacity is reduced, like in the presence of pathologies like diabetes, vascular diseases, chronic infection, and others.
34432331	3	44	theme	persistent	476:485	arg1	inflammation					487:498	persistent inflammation	476:498	persistent inflammation	476:498	However, when persistent inflammation occurs, the physiological tissue healing capacity is reduced, like in the presence of pathologies like diabetes, vascular diseases, chronic infection, and others.
34432331	8	45	theme	3D-printed	1326:1335	arg1	scaffolds					1359:1367	3D-printed alginate and chitosan scaffolds	1326:1367	3D-printed alginate and chitosan scaffolds	1326:1367	In the present study, 3D-printed alginate and chitosan scaffolds were combined with the HELP components.
34432331	2	46	theme	additive	264:271	arg1	technologies					287:298	Three-dimensional (3D) advanced additive manufacturing technologies	232:298	Three-dimensional (3D) advanced additive manufacturing technologies	232:298	Three-dimensional (3D) advanced additive manufacturing technologies allow creating scaffolds with controlled and reproducible macro- and micro-structure that improve the quality of the restored tissue to favor spontaneous repair.
34432331	3	47	theme	tissue	526:531	arg1	capacity					541:548	the physiological tissue healing capacity	508:548	the physiological tissue healing capacity	508:548	However, when persistent inflammation occurs, the physiological tissue healing capacity is reduced, like in the presence of pathologies like diabetes, vascular diseases, chronic infection, and others.
34432331	8	48	theme	chitosan	1350:1357	arg1	scaffolds					1359:1367	3D-printed alginate and chitosan scaffolds	1326:1367	3D-printed alginate and chitosan scaffolds	1326:1367	In the present study, 3D-printed alginate and chitosan scaffolds were combined with the HELP components.
34432331	7	49	with	endowed	1187:1193	arg1	functionality					1211:1223	biological functionality	1200:1223	biological functionality	1200:1223	Their structure allows the integration of domains endowed with biological functionality, making them attractive compounds to prepare composites with smart properties.
34432331	2	50	theme	advanced	255:262	arg1	technologies					287:298	Three-dimensional (3D) advanced additive manufacturing technologies	232:298	Three-dimensional (3D) advanced additive manufacturing technologies	232:298	Three-dimensional (3D) advanced additive manufacturing technologies allow creating scaffolds with controlled and reproducible macro- and micro-structure that improve the quality of the restored tissue to favor spontaneous repair.
34432331	0	51	theme	local	55:59	arg1	delivery					61:68	the stimuli-induced local delivery	35:68	the stimuli-induced local delivery of bioactive adjuncts	35:90	3D-printed scaffold composites for the stimuli-induced local delivery of bioactive adjuncts.
34432331	2	52	with	scaffolds	315:323	arg1	micro-structure					369:383	micro-structure	369:383	micro-structure	369:383	Three-dimensional (3D) advanced additive manufacturing technologies allow creating scaffolds with controlled and reproducible macro- and micro-structure that improve the quality of the restored tissue to favor spontaneous repair.
34432331	2	52	with	scaffolds	315:323	arg1	macro-					358:363	macro-	358:363	macro-	358:363	Three-dimensional (3D) advanced additive manufacturing technologies allow creating scaffolds with controlled and reproducible macro- and micro-structure that improve the quality of the restored tissue to favor spontaneous repair.
34432331	10	53	theme	smart	1645:1649	arg1	composites					1661:1670	these smart bioactive composites	1639:1670	these smart bioactive composites	1639:1670	Different constructs were prepared and the stimuli-responsive behavior as well as the biological activity were evaluated, suggesting that these smart bioactive composites are suitable to realize multifunctional dressings that sustain the local release of therapeutic adjuncts.
34432331	10	54	theme	biological	1587:1596	arg1	activity					1598:1605	the biological activity	1583:1605	the stimuli-responsive behavior as well as the biological activity	1540:1605	Different constructs were prepared and the stimuli-responsive behavior as well as the biological activity were evaluated, suggesting that these smart bioactive composites are suitable to realize multifunctional dressings that sustain the local release of therapeutic adjuncts.
34432331	4	55	dep	therapeutic	710:720	arg1	adjuncts					722:729	adjuncts	722:729	adjuncts	722:729	In these circumstances, the bioavailability of therapeutic adjuncts like the growth factors in addition to the standard treatments represents undoubtedly a promising strategy to accelerate the healing of skin lesions.
34432331	3	56	theme	vascular	613:620	arg1	diseases					622:629	vascular diseases	613:629	vascular diseases	613:629	However, when persistent inflammation occurs, the physiological tissue healing capacity is reduced, like in the presence of pathologies like diabetes, vascular diseases, chronic infection, and others.
34432331	2	57	dep	Three-dimensional	232:248	arg1	3D					251:252	3D	251:252	3D	251:252	Three-dimensional (3D) advanced additive manufacturing technologies allow creating scaffolds with controlled and reproducible macro- and micro-structure that improve the quality of the restored tissue to favor spontaneous repair.
34432331	2	58	theme	Three-dimensional	232:248	arg1	technologies					287:298	Three-dimensional (3D) advanced additive manufacturing technologies	232:298	Three-dimensional (3D) advanced additive manufacturing technologies	232:298	Three-dimensional (3D) advanced additive manufacturing technologies allow creating scaffolds with controlled and reproducible macro- and micro-structure that improve the quality of the restored tissue to favor spontaneous repair.
34432331	2	59	theme	creating	306:313	arg1	scaffolds					315:323	creating scaffolds	306:323	creating scaffolds with controlled and reproducible macro- and micro-structure that improve the quality of the restored tissue to favor spontaneous repair	306:459	Three-dimensional (3D) advanced additive manufacturing technologies allow creating scaffolds with controlled and reproducible macro- and micro-structure that improve the quality of the restored tissue to favor spontaneous repair.
34432331	4	60	theme	growth	740:745	arg1	factors					747:753	the growth factors	736:753	the growth factors in addition to the standard treatments	736:792	In these circumstances, the bioavailability of therapeutic adjuncts like the growth factors in addition to the standard treatments represents undoubtedly a promising strategy to accelerate the healing of skin lesions.
34432331	8	61	theme	present	1311:1317	arg1	study					1319:1323	the present study	1307:1323	the present study	1307:1323	In the present study, 3D-printed alginate and chitosan scaffolds were combined with the HELP components.
34432331	0	62	theme	bioactive	73:81	arg1	delivery					61:68	the stimuli-induced local delivery	35:68	the stimuli-induced local delivery of bioactive adjuncts	35:90	3D-printed scaffold composites for the stimuli-induced local delivery of bioactive adjuncts.
34432331	5	63	theme	3D	937:938	arg1	printing					940:947	3D printing	937:947	3D printing	937:947	Precisely designed polysaccharide scaffolds obtained by 3D printing represent a robust platform that can be further implemented with the controlled delivery of bioactive adjuncts.
34432331	4	64	theme	lesions	872:878	arg1	healing					856:862	the healing	852:862	the healing of skin lesions	852:878	In these circumstances, the bioavailability of therapeutic adjuncts like the growth factors in addition to the standard treatments represents undoubtedly a promising strategy to accelerate the healing of skin lesions.
34432331	7	65	with	composites	1270:1279	arg1	properties					1292:1301	smart properties	1286:1301	smart properties	1286:1301	Their structure allows the integration of domains endowed with biological functionality, making them attractive compounds to prepare composites with smart properties.
34432331	6	66	theme	stimuli-responsive	1105:1122	arg1	polypeptides					1080:1091	Human elastin-like polypeptides	1061:1091	Human elastin-like polypeptides (HELPs)	1061:1099	Human elastin-like polypeptides (HELPs) are stimuli-responsive biopolymers.
34432331	6	66	theme	stimuli-responsive	1105:1122	arg1	biopolymers					1124:1134	stimuli-responsive biopolymers	1105:1134	stimuli-responsive biopolymers	1105:1134	Human elastin-like polypeptides (HELPs) are stimuli-responsive biopolymers.
34432331	10	67	theme	therapeutic	1756:1766	arg1	release					1745:1751	the local release	1735:1751	the local release of therapeutic adjuncts	1735:1775	Different constructs were prepared and the stimuli-responsive behavior as well as the biological activity were evaluated, suggesting that these smart bioactive composites are suitable to realize multifunctional dressings that sustain the local release of therapeutic adjuncts.
34432331	4	68	theme	skin	867:870	arg1	lesions					872:878	skin lesions	867:878	skin lesions	867:878	In these circumstances, the bioavailability of therapeutic adjuncts like the growth factors in addition to the standard treatments represents undoubtedly a promising strategy to accelerate the healing of skin lesions.
34432331	2	69	theme	tissue	426:431	arg1	quality					402:408	the quality	398:408	the quality of the restored tissue to favor spontaneous repair	398:459	Three-dimensional (3D) advanced additive manufacturing technologies allow creating scaffolds with controlled and reproducible macro- and micro-structure that improve the quality of the restored tissue to favor spontaneous repair.
34432331	9	70	dep	biopolymer	1418:1427	arg1	HELP					1413:1416	HELP	1413:1416	HELP	1413:1416	The HELP biopolymer was fused to the epidermal growth factor (EGF) as the bioactive domain.
32128391	0	0	theme	genes	69:73	arg1	network					58:64	a large network	50:64	a large network of genes pleiotropic with inflammatory diseases	50:112	Glycosylation of immunoglobulin G is regulated by a large network of genes pleiotropic with inflammatory diseases.
32128391	2	1	gly	glycosylation	268:280	arg1	IgG					285:287	IgG	285:287	IgG	285:287	Aberrant glycosylation of IgG has been observed in many diseases, but little is understood about the underlying mechanisms.
32128391	2	1	gly	glycosylation	268:280	arg1	diseases					315:322	many diseases	310:322	many diseases	310:322	Aberrant glycosylation of IgG has been observed in many diseases, but little is understood about the underlying mechanisms.
32128391	6	2	contain	has	1149:1151	arg1	variation					1031:1039	variation	1031:1039	variation in key transcription factors coupled with regulatory variation in glycogenes	1031:1116	This study provides new evidence that variation in key transcription factors coupled with regulatory variation in glycogenes modifies IgG glycosylation and has influence on inflammatory diseases.
32128391	6	2	contain	has	1149:1151	arg2	influence					1153:1161	influence	1153:1161	influence on inflammatory diseases	1153:1186	This study provides new evidence that variation in key transcription factors coupled with regulatory variation in glycogenes modifies IgG glycosylation and has influence on inflammatory diseases.
32128391	4	3	theme	FUT8	655:658	arg1	expression					622:631	the expression	618:631	the expression of fucosyltransferase FUT8, resulting in increased levels of fucosylated glycans,	618:713	We confirmed in vitro that knockdown of IKZF1 decreases the expression of fucosyltransferase FUT8, resulting in increased levels of fucosylated glycans, and suggest that RUNX1 and RUNX3, together with SMARCB1, regulate expression of glycosyltransferase MGAT3.
32128391	4	4	theme	MGAT3	815:819	arg1	expression					781:790	expression	781:790	expression of glycosyltransferase MGAT3	781:819	We confirmed in vitro that knockdown of IKZF1 decreases the expression of fucosyltransferase FUT8, resulting in increased levels of fucosylated glycans, and suggest that RUNX1 and RUNX3, together with SMARCB1, regulate expression of glycosyltransferase MGAT3.
32128391	6	5	from	variation	1031:1039	arg1	factors					1062:1068	key transcription factors	1044:1068	key transcription factors coupled with regulatory variation in glycogenes	1044:1116	This study provides new evidence that variation in key transcription factors coupled with regulatory variation in glycogenes modifies IgG glycosylation and has influence on inflammatory diseases.
32128391	4	6	theme	fucosyltransferase	636:653	arg1	FUT8					655:658	fucosyltransferase FUT8	636:658	fucosyltransferase FUT8	636:658	We confirmed in vitro that knockdown of IKZF1 decreases the expression of fucosyltransferase FUT8, resulting in increased levels of fucosylated glycans, and suggest that RUNX1 and RUNX3, together with SMARCB1, regulate expression of glycosyltransferase MGAT3.
32128391	2	7	theme	Aberrant	259:266	arg1	glycosylation					268:280	Aberrant glycosylation	259:280	Aberrant glycosylation of IgG	259:287	Aberrant glycosylation of IgG has been observed in many diseases, but little is understood about the underlying mechanisms.
32128391	0	8	theme	pleiotropic	75:85	arg1	genes					69:73	genes	69:73	genes pleiotropic with inflammatory diseases	69:112	Glycosylation of immunoglobulin G is regulated by a large network of genes pleiotropic with inflammatory diseases.
32128391	5	9	theme	glycoenzymes	913:924	arg1	regulation					899:908	the regulation	895:908	the regulation of glycoenzymes	895:924	We also show that variants affecting the expression of genes involved in the regulation of glycoenzymes colocalize with variants affecting risk for inflammatory diseases.
32128391	6	10	from	influence	1153:1161	arg1	diseases					1179:1186	inflammatory diseases	1166:1186	inflammatory diseases	1166:1186	This study provides new evidence that variation in key transcription factors coupled with regulatory variation in glycogenes modifies IgG glycosylation and has influence on inflammatory diseases.
32128391	6	11	theme	regulatory	1083:1092	arg1	variation					1094:1102	regulatory variation	1083:1102	regulatory variation in glycogenes	1083:1116	This study provides new evidence that variation in key transcription factors coupled with regulatory variation in glycogenes modifies IgG glycosylation and has influence on inflammatory diseases.
32128391	4	12	gly	fucosylated	694:704	arg1	glycans					706:712	fucosylated glycans	694:712	fucosylated glycans	694:712	We confirmed in vitro that knockdown of IKZF1 decreases the expression of fucosyltransferase FUT8, resulting in increased levels of fucosylated glycans, and suggest that RUNX1 and RUNX3, together with SMARCB1, regulate expression of glycosyltransferase MGAT3.
32128391	3	13	theme	=	454:454	arg1	N					452:452	N = 8090	452:459	N = 8090	452:459	We performed a genome-wide association study of IgG N-glycosylation (N = 8090) and, using a data-driven network approach, suggested how associated loci form a functional network.
32128391	3	13	theme	=	454:454	arg1	N-glycosylation					435:449	IgG N-glycosylation	431:449	IgG N-glycosylation (N = 8090)	431:460	We performed a genome-wide association study of IgG N-glycosylation (N = 8090) and, using a data-driven network approach, suggested how associated loci form a functional network.
32128391	6	14	theme	new	1013:1015	arg1	evidence					1017:1024	new evidence that variation in key transcription factors coupled with regulatory variation in glycogenes modifies IgG glycosylation and has influence on inflammatory diseases	1013:1186	new evidence that variation in key transcription factors coupled with regulatory variation in glycogenes modifies IgG glycosylation and has influence on inflammatory diseases	1013:1186	This study provides new evidence that variation in key transcription factors coupled with regulatory variation in glycogenes modifies IgG glycosylation and has influence on inflammatory diseases.
32128391	3	15	theme	genome-wide	398:408	arg1	study					422:426	a genome-wide association study	396:426	a genome-wide association study of IgG N-glycosylation (N = 8090)	396:460	We performed a genome-wide association study of IgG N-glycosylation (N = 8090) and, using a data-driven network approach, suggested how associated loci form a functional network.
32128391	0	16	theme	inflammatory	92:103	arg1	diseases					105:112	inflammatory diseases	92:112	inflammatory diseases	92:112	Glycosylation of immunoglobulin G is regulated by a large network of genes pleiotropic with inflammatory diseases.
32128391	6	17	theme	key	1044:1046	arg1	factors					1062:1068	key transcription factors	1044:1068	key transcription factors coupled with regulatory variation in glycogenes	1044:1116	This study provides new evidence that variation in key transcription factors coupled with regulatory variation in glycogenes modifies IgG glycosylation and has influence on inflammatory diseases.
32128391	3	18	theme	data-driven	475:485	arg1	approach					495:502	a data-driven network approach	473:502	a data-driven network approach	473:502	We performed a genome-wide association study of IgG N-glycosylation (N = 8090) and, using a data-driven network approach, suggested how associated loci form a functional network.
32128391	6	19	theme	transcription	1048:1060	arg1	factors					1062:1068	key transcription factors	1044:1068	key transcription factors coupled with regulatory variation in glycogenes	1044:1116	This study provides new evidence that variation in key transcription factors coupled with regulatory variation in glycogenes modifies IgG glycosylation and has influence on inflammatory diseases.
32128391	3	20	theme	association	410:420	arg1	study					422:426	a genome-wide association study	396:426	a genome-wide association study of IgG N-glycosylation (N = 8090)	396:460	We performed a genome-wide association study of IgG N-glycosylation (N = 8090) and, using a data-driven network approach, suggested how associated loci form a functional network.
32128391	2	21	located	observed	298:305	arg2	glycosylation					268:280	Aberrant glycosylation	259:280	Aberrant glycosylation of IgG	259:287	Aberrant glycosylation of IgG has been observed in many diseases, but little is understood about the underlying mechanisms.
32128391	2	21	located	observed	298:305	arg1	diseases					315:322	many diseases	310:322	many diseases	310:322	Aberrant glycosylation of IgG has been observed in many diseases, but little is understood about the underlying mechanisms.
32128391	4	22	theme	glycosyltransferase	795:813	arg1	MGAT3					815:819	glycosyltransferase MGAT3	795:819	glycosyltransferase MGAT3	795:819	We confirmed in vitro that knockdown of IKZF1 decreases the expression of fucosyltransferase FUT8, resulting in increased levels of fucosylated glycans, and suggest that RUNX1 and RUNX3, together with SMARCB1, regulate expression of glycosyltransferase MGAT3.
32128391	1	23	theme	immune	244:249	arg1	system					251:256	the immune system	240:256	the immune system	240:256	Effector functions of immunoglobulin G (IgG) are regulated by the composition of a glycan moiety, thus affecting activity of the immune system.
32128391	2	24	theme	IgG	285:287	arg1	glycosylation					268:280	Aberrant glycosylation	259:280	Aberrant glycosylation of IgG	259:287	Aberrant glycosylation of IgG has been observed in many diseases, but little is understood about the underlying mechanisms.
32128391	0	25	theme	G	32:32	arg1	Glycosylation					0:12	Glycosylation	0:12	Glycosylation of immunoglobulin G	0:32	Glycosylation of immunoglobulin G is regulated by a large network of genes pleiotropic with inflammatory diseases.
32128391	1	26	theme	system	251:256	arg1	activity					228:235	activity	228:235	activity of the immune system	228:256	Effector functions of immunoglobulin G (IgG) are regulated by the composition of a glycan moiety, thus affecting activity of the immune system.
32128391	0	27	with	pleiotropic	75:85	arg1	diseases					105:112	inflammatory diseases	92:112	inflammatory diseases	92:112	Glycosylation of immunoglobulin G is regulated by a large network of genes pleiotropic with inflammatory diseases.
32128391	4	28	theme	glycans	706:712	arg1	levels					684:689	increased levels	674:689	increased levels of fucosylated glycans	674:712	We confirmed in vitro that knockdown of IKZF1 decreases the expression of fucosyltransferase FUT8, resulting in increased levels of fucosylated glycans, and suggest that RUNX1 and RUNX3, together with SMARCB1, regulate expression of glycosyltransferase MGAT3.
32128391	5	29	theme	inflammatory	970:981	arg1	diseases					983:990	inflammatory diseases	970:990	inflammatory diseases	970:990	We also show that variants affecting the expression of genes involved in the regulation of glycoenzymes colocalize with variants affecting risk for inflammatory diseases.
32128391	3	30	theme	IgG	431:433	arg1	N					452:452	N = 8090	452:459	N = 8090	452:459	We performed a genome-wide association study of IgG N-glycosylation (N = 8090) and, using a data-driven network approach, suggested how associated loci form a functional network.
32128391	3	30	theme	IgG	431:433	arg1	N-glycosylation					435:449	IgG N-glycosylation	431:449	IgG N-glycosylation (N = 8090)	431:460	We performed a genome-wide association study of IgG N-glycosylation (N = 8090) and, using a data-driven network approach, suggested how associated loci form a functional network.
32128391	0	31	theme	immunoglobulin	17:30	arg1	G					32:32	immunoglobulin G	17:32	immunoglobulin G	17:32	Glycosylation of immunoglobulin G is regulated by a large network of genes pleiotropic with inflammatory diseases.
32128391	6	32	theme	inflammatory	1166:1177	arg1	diseases					1179:1186	inflammatory diseases	1166:1186	inflammatory diseases	1166:1186	This study provides new evidence that variation in key transcription factors coupled with regulatory variation in glycogenes modifies IgG glycosylation and has influence on inflammatory diseases.
32128391	4	33	theme	fucosylated	694:704	arg1	glycans					706:712	fucosylated glycans	694:712	fucosylated glycans	694:712	We confirmed in vitro that knockdown of IKZF1 decreases the expression of fucosyltransferase FUT8, resulting in increased levels of fucosylated glycans, and suggest that RUNX1 and RUNX3, together with SMARCB1, regulate expression of glycosyltransferase MGAT3.
32128391	3	34	theme	N-glycosylation	435:449	arg1	study					422:426	a genome-wide association study	396:426	a genome-wide association study of IgG N-glycosylation (N = 8090)	396:460	We performed a genome-wide association study of IgG N-glycosylation (N = 8090) and, using a data-driven network approach, suggested how associated loci form a functional network.
32128391	0	35	gly	Glycosylation	0:12	arg1	G					32:32	immunoglobulin G	17:32	immunoglobulin G	17:32	Glycosylation of immunoglobulin G is regulated by a large network of genes pleiotropic with inflammatory diseases.
32128391	3	36	theme	associated	519:528	arg1	loci					530:533	associated loci	519:533	associated loci	519:533	We performed a genome-wide association study of IgG N-glycosylation (N = 8090) and, using a data-driven network approach, suggested how associated loci form a functional network.
32128391	4	37	theme	IKZF1	602:606	arg1	knockdown					589:597	knockdown	589:597	knockdown of IKZF1	589:606	We confirmed in vitro that knockdown of IKZF1 decreases the expression of fucosyltransferase FUT8, resulting in increased levels of fucosylated glycans, and suggest that RUNX1 and RUNX3, together with SMARCB1, regulate expression of glycosyltransferase MGAT3.
32128391	3	38	theme	functional	542:551	arg1	network					553:559	a functional network	540:559	a functional network	540:559	We performed a genome-wide association study of IgG N-glycosylation (N = 8090) and, using a data-driven network approach, suggested how associated loci form a functional network.
32128391	6	39	from	variation	1094:1102	arg1	glycogenes					1107:1116	glycogenes	1107:1116	glycogenes	1107:1116	This study provides new evidence that variation in key transcription factors coupled with regulatory variation in glycogenes modifies IgG glycosylation and has influence on inflammatory diseases.
32128391	2	40	theme	underlying	360:369	arg1	mechanisms					371:380	the underlying mechanisms	356:380	the underlying mechanisms	356:380	Aberrant glycosylation of IgG has been observed in many diseases, but little is understood about the underlying mechanisms.
32128391	2	41	theme	many	310:313	arg1	diseases					315:322	many diseases	310:322	many diseases	310:322	Aberrant glycosylation of IgG has been observed in many diseases, but little is understood about the underlying mechanisms.
32128391	1	42	theme	Effector	115:122	arg1	functions					124:132	Effector functions	115:132	Effector functions of immunoglobulin G (IgG)	115:158	Effector functions of immunoglobulin G (IgG) are regulated by the composition of a glycan moiety, thus affecting activity of the immune system.
32128391	4	43	theme	increased	674:682	arg1	levels					684:689	increased levels	674:689	increased levels of fucosylated glycans	674:712	We confirmed in vitro that knockdown of IKZF1 decreases the expression of fucosyltransferase FUT8, resulting in increased levels of fucosylated glycans, and suggest that RUNX1 and RUNX3, together with SMARCB1, regulate expression of glycosyltransferase MGAT3.
32128391	6	44	theme	IgG	1127:1129	arg1	glycosylation					1131:1143	IgG glycosylation	1127:1143	IgG glycosylation	1127:1143	This study provides new evidence that variation in key transcription factors coupled with regulatory variation in glycogenes modifies IgG glycosylation and has influence on inflammatory diseases.
32128391	1	45	theme	glycan	198:203	arg1	moiety					205:210	a glycan moiety	196:210	a glycan moiety	196:210	Effector functions of immunoglobulin G (IgG) are regulated by the composition of a glycan moiety, thus affecting activity of the immune system.
32128391	3	46	theme	network	487:493	arg1	approach					495:502	a data-driven network approach	473:502	a data-driven network approach	473:502	We performed a genome-wide association study of IgG N-glycosylation (N = 8090) and, using a data-driven network approach, suggested how associated loci form a functional network.
32128391	5	47	theme	genes	877:881	arg1	expression					863:872	the expression	859:872	the expression of genes involved in the regulation of glycoenzymes	859:924	We also show that variants affecting the expression of genes involved in the regulation of glycoenzymes colocalize with variants affecting risk for inflammatory diseases.
32128391	1	48	theme	moiety	205:210	arg1	composition					181:191	the composition	177:191	the composition of a glycan moiety	177:210	Effector functions of immunoglobulin G (IgG) are regulated by the composition of a glycan moiety, thus affecting activity of the immune system.
32128391	4	49	dep	together	749:756	arg1	with					758:761	with	758:761	with	758:761	We confirmed in vitro that knockdown of IKZF1 decreases the expression of fucosyltransferase FUT8, resulting in increased levels of fucosylated glycans, and suggest that RUNX1 and RUNX3, together with SMARCB1, regulate expression of glycosyltransferase MGAT3.
32128391	1	50	theme	immunoglobulin	137:150	arg1	G					152:152	immunoglobulin G	137:152	immunoglobulin G (IgG)	137:158	Effector functions of immunoglobulin G (IgG) are regulated by the composition of a glycan moiety, thus affecting activity of the immune system.
32128391	1	50	theme	immunoglobulin	137:150	arg1	IgG					155:157	IgG	155:157	IgG	155:157	Effector functions of immunoglobulin G (IgG) are regulated by the composition of a glycan moiety, thus affecting activity of the immune system.
32128391	0	51	theme	large	52:56	arg1	network					58:64	a large network	50:64	a large network of genes pleiotropic with inflammatory diseases	50:112	Glycosylation of immunoglobulin G is regulated by a large network of genes pleiotropic with inflammatory diseases.
32128391	1	52	theme	G	152:152	arg1	functions					124:132	Effector functions	115:132	Effector functions of immunoglobulin G (IgG)	115:158	Effector functions of immunoglobulin G (IgG) are regulated by the composition of a glycan moiety, thus affecting activity of the immune system.
34920059	6	0	dep	showed	920:925	arg1	increased					1026:1034	increased	1026:1034	showed good antibacterial activity against Escherichia coli (E. coli) bacteria and its bacterial activity increased with Cu doping	920:1049	Besides this, CNC/NiO composite showed good antibacterial activity against Escherichia coli (E. coli) bacteria and its bacterial activity increased with Cu doping.
34920059	5	1	theme	Cu-doped	686:693	arg1	CNC/NiO					695:701	Cu-doped CNC/NiO	686:701	Cu-doped CNC/NiO	686:701	Both CNC/NiO composite and Cu-doped CNC/NiO showed higher catalytic potential compared to dopant-free NiO, while Cu-doped CNC/NiO nanostructures exhibited significant potential for use in industrial dye degradation applications.
34920059	7	2	theme	gyrase	1135:1140	arg1	enzyme					1142:1147	dihydrofolate reductase and DNA gyrase enzyme	1103:1147	dihydrofolate reductase and DNA gyrase enzyme	1103:1147	Furthermore, molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme confirmed interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets and showed good agreement with in vitro bactericidal activity.
34920059	7	3	theme	CNC/NiO	1204:1210	arg1	pockets					1226:1232	Cu-doped CNC/NiO inside active pockets	1195:1232	Cu-doped CNC/NiO inside active pockets	1195:1232	Furthermore, molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme confirmed interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets and showed good agreement with in vitro bactericidal activity.
34920059	7	4	theme	active	1219:1224	arg1	pockets					1226:1232	Cu-doped CNC/NiO inside active pockets	1195:1232	Cu-doped CNC/NiO inside active pockets	1195:1232	Furthermore, molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme confirmed interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets and showed good agreement with in vitro bactericidal activity.
34920059	6	5	theme	good	927:930	arg1	activity					946:953	good antibacterial activity	927:953	good antibacterial activity against Escherichia coli (E. coli) bacteria and its bacterial activity	927:1024	Besides this, CNC/NiO composite showed good antibacterial activity against Escherichia coli (E. coli) bacteria and its bacterial activity increased with Cu doping.
34920059	4	6	theme	doped	604:608	arg1	nanostructures					610:623	doped nanostructures	604:623	doped nanostructures	604:623	The morphology as well as interlayer spacing was evaluated with HRTEM while functional groups presence in dopant-free and doped nanostructures was confirmed using FTIR spectra.
34920059	5	7	theme	Cu-doped	772:779	arg1	nanostructures					789:802	Cu-doped CNC/NiO nanostructures	772:802	Cu-doped CNC/NiO nanostructures	772:802	Both CNC/NiO composite and Cu-doped CNC/NiO showed higher catalytic potential compared to dopant-free NiO, while Cu-doped CNC/NiO nanostructures exhibited significant potential for use in industrial dye degradation applications.
34920059	3	8	from	increase	440:447	arg1	size					464:467	crystallite size	452:467	crystallite size with doping	452:479	XRD patterns confirmed rhombohedral structure of synthesized nanostructures with gradual increase in crystallite size with doping.
34920059	7	9	theme	Cu-doped	1195:1202	arg1	pockets					1226:1232	Cu-doped CNC/NiO inside active pockets	1195:1232	Cu-doped CNC/NiO inside active pockets	1195:1232	Furthermore, molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme confirmed interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets and showed good agreement with in vitro bactericidal activity.
34920059	0	10	theme	cellulose	92:100	arg1	nanocrystals					102:113	cellulose nanocrystals	92:113	cellulose nanocrystals	92:113	Bactericidal action and molecular docking studies of catalytic Cu-doped NiO composited with cellulose nanocrystals.
34920059	5	11	theme	higher	710:715	arg1	potential					727:735	higher catalytic potential	710:735	higher catalytic potential	710:735	Both CNC/NiO composite and Cu-doped CNC/NiO showed higher catalytic potential compared to dopant-free NiO, while Cu-doped CNC/NiO nanostructures exhibited significant potential for use in industrial dye degradation applications.
34920059	2	12	theme	catalytic	275:283	arg1	studies					322:328	the catalytic, antibacterial and molecular docking studies	271:328	the catalytic, antibacterial and molecular docking studies of prepared samples	271:348	The aim of this study is to investigate the catalytic, antibacterial and molecular docking studies of prepared samples.
34920059	6	13	dep	bacteria	990:997	arg1	coli					984:987	E. coli	981:987	E. coli	981:987	Besides this, CNC/NiO composite showed good antibacterial activity against Escherichia coli (E. coli) bacteria and its bacterial activity increased with Cu doping.
34920059	7	14	theme	bactericidal	1274:1285	arg1	activity					1287:1294	in vitro bactericidal activity	1265:1294	in vitro bactericidal activity	1265:1294	Furthermore, molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme confirmed interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets and showed good agreement with in vitro bactericidal activity.
34920059	7	15	theme	CNC/NiO	1183:1189	arg1	interaction					1159:1169	interaction	1159:1169	interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets	1159:1232	Furthermore, molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme confirmed interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets and showed good agreement with in vitro bactericidal activity.
34920059	3	16	theme	XRD	351:353	arg1	patterns					355:362	XRD patterns	351:362	XRD patterns	351:362	XRD patterns confirmed rhombohedral structure of synthesized nanostructures with gradual increase in crystallite size with doping.
34920059	4	17	theme	interlayer	508:517	arg1	spacing					519:525	interlayer spacing	508:525	The morphology as well as interlayer spacing	482:525	The morphology as well as interlayer spacing was evaluated with HRTEM while functional groups presence in dopant-free and doped nanostructures was confirmed using FTIR spectra.
34920059	5	18	theme	CNC/NiO	664:670	arg1	composite					672:680	CNC/NiO composite	664:680	CNC/NiO composite	664:680	Both CNC/NiO composite and Cu-doped CNC/NiO showed higher catalytic potential compared to dopant-free NiO, while Cu-doped CNC/NiO nanostructures exhibited significant potential for use in industrial dye degradation applications.
34920059	7	19	theme	pockets	1226:1232	arg1	interaction					1159:1169	interaction	1159:1169	interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets	1159:1232	Furthermore, molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme confirmed interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets and showed good agreement with in vitro bactericidal activity.
34920059	3	20	theme	synthesized	400:410	arg1	nanostructures					412:425	synthesized nanostructures	400:425	synthesized nanostructures	400:425	XRD patterns confirmed rhombohedral structure of synthesized nanostructures with gradual increase in crystallite size with doping.
34920059	6	21	theme	bacterial	1007:1015	arg1	activity					1017:1024	its bacterial activity	1003:1024	its bacterial activity	1003:1024	Besides this, CNC/NiO composite showed good antibacterial activity against Escherichia coli (E. coli) bacteria and its bacterial activity increased with Cu doping.
34920059	7	22	theme	in	1265:1266	arg1	activity					1287:1294	in vitro bactericidal activity	1265:1294	in vitro bactericidal activity	1265:1294	Furthermore, molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme confirmed interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets and showed good agreement with in vitro bactericidal activity.
34920059	6	23	theme	Escherichia	963:973	arg1	bacteria					990:997	Escherichia coli (E. coli) bacteria	963:997	Escherichia coli (E. coli) bacteria	963:997	Besides this, CNC/NiO composite showed good antibacterial activity against Escherichia coli (E. coli) bacteria and its bacterial activity increased with Cu doping.
34920059	7	24	theme	NPs	1178:1180	arg1	interaction					1159:1169	interaction	1159:1169	interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets	1159:1232	Furthermore, molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme confirmed interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets and showed good agreement with in vitro bactericidal activity.
34920059	5	25	theme	significant	814:824	arg1	potential					826:834	significant potential	814:834	significant potential for use in industrial dye degradation applications	814:885	Both CNC/NiO composite and Cu-doped CNC/NiO showed higher catalytic potential compared to dopant-free NiO, while Cu-doped CNC/NiO nanostructures exhibited significant potential for use in industrial dye degradation applications.
34920059	2	26	theme	docking	314:320	arg1	studies					322:328	the catalytic, antibacterial and molecular docking studies	271:328	the catalytic, antibacterial and molecular docking studies of prepared samples	271:348	The aim of this study is to investigate the catalytic, antibacterial and molecular docking studies of prepared samples.
34920059	0	27	theme	Bactericidal	0:11	arg1	action					13:18	Bactericidal action	0:18	Bactericidal action	0:18	Bactericidal action and molecular docking studies of catalytic Cu-doped NiO composited with cellulose nanocrystals.
34920059	7	28	theme	good	1245:1248	arg1	agreement					1250:1258	good agreement	1245:1258	good agreement with in vitro bactericidal activity	1245:1294	Furthermore, molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme confirmed interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets and showed good agreement with in vitro bactericidal activity.
34920059	2	29	theme	molecular	304:312	arg1	studies					322:328	the catalytic, antibacterial and molecular docking studies	271:328	the catalytic, antibacterial and molecular docking studies of prepared samples	271:348	The aim of this study is to investigate the catalytic, antibacterial and molecular docking studies of prepared samples.
34920059	3	30	theme	crystallite	452:462	arg1	size					464:467	crystallite size	452:467	crystallite size with doping	452:479	XRD patterns confirmed rhombohedral structure of synthesized nanostructures with gradual increase in crystallite size with doping.
34920059	0	31	theme	molecular	24:32	arg1	studies					42:48	molecular docking studies	24:48	molecular docking studies	24:48	Bactericidal action and molecular docking studies of catalytic Cu-doped NiO composited with cellulose nanocrystals.
34920059	5	32	theme	dopant-free	749:759	arg1	NiO					761:763	dopant-free NiO	749:763	dopant-free NiO	749:763	Both CNC/NiO composite and Cu-doped CNC/NiO showed higher catalytic potential compared to dopant-free NiO, while Cu-doped CNC/NiO nanostructures exhibited significant potential for use in industrial dye degradation applications.
34920059	5	33	theme	dye	858:860	arg1	applications					874:885	industrial dye degradation applications	847:885	industrial dye degradation applications	847:885	Both CNC/NiO composite and Cu-doped CNC/NiO showed higher catalytic potential compared to dopant-free NiO, while Cu-doped CNC/NiO nanostructures exhibited significant potential for use in industrial dye degradation applications.
34920059	4	34	from	presence	576:583	arg1	dopant-free					588:598	dopant-free	588:598	dopant-free	588:598	The morphology as well as interlayer spacing was evaluated with HRTEM while functional groups presence in dopant-free and doped nanostructures was confirmed using FTIR spectra.
34920059	4	34	from	presence	576:583	arg1	nanostructures					610:623	doped nanostructures	604:623	doped nanostructures	604:623	The morphology as well as interlayer spacing was evaluated with HRTEM while functional groups presence in dopant-free and doped nanostructures was confirmed using FTIR spectra.
34920059	6	35	theme	Cu	1041:1042	arg1	doping					1044:1049	Cu doping	1041:1049	Cu doping	1041:1049	Besides this, CNC/NiO composite showed good antibacterial activity against Escherichia coli (E. coli) bacteria and its bacterial activity increased with Cu doping.
34920059	7	36	theme	NiO	1174:1176	arg1	NPs					1178:1180	NiO NPs	1174:1180	NiO NPs	1174:1180	Furthermore, molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme confirmed interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets and showed good agreement with in vitro bactericidal activity.
34920059	2	37	theme	antibacterial	286:298	arg1	studies					322:328	the catalytic, antibacterial and molecular docking studies	271:328	the catalytic, antibacterial and molecular docking studies of prepared samples	271:348	The aim of this study is to investigate the catalytic, antibacterial and molecular docking studies of prepared samples.
34920059	3	38	theme	gradual	432:438	arg1	increase					440:447	gradual increase	432:447	gradual increase in crystallite size with doping	432:479	XRD patterns confirmed rhombohedral structure of synthesized nanostructures with gradual increase in crystallite size with doping.
34920059	2	39	theme	prepared	333:340	arg1	samples					342:348	prepared samples	333:348	prepared samples	333:348	The aim of this study is to investigate the catalytic, antibacterial and molecular docking studies of prepared samples.
34920059	7	40	dep	in	1265:1266	arg1	vitro					1268:1272	vitro	1268:1272	vitro	1268:1272	Furthermore, molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme confirmed interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets and showed good agreement with in vitro bactericidal activity.
34920059	1	41	theme	co-precipitation	206:221	arg1	method					223:228	co-precipitation method	206:228	co-precipitation method	206:228	Synthesis of Cu-doped NiO composited with cellulose nanocrystals (CNC) was carried out by co-precipitation method.
34920059	0	42	theme	docking	34:40	arg1	studies					42:48	molecular docking studies	24:48	molecular docking studies	24:48	Bactericidal action and molecular docking studies of catalytic Cu-doped NiO composited with cellulose nanocrystals.
34920059	7	43	theme	inside	1212:1217	arg1	pockets					1226:1232	Cu-doped CNC/NiO inside active pockets	1195:1232	Cu-doped CNC/NiO inside active pockets	1195:1232	Furthermore, molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme confirmed interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets and showed good agreement with in vitro bactericidal activity.
34920059	5	44	theme	industrial	847:856	arg1	applications					874:885	industrial dye degradation applications	847:885	industrial dye degradation applications	847:885	Both CNC/NiO composite and Cu-doped CNC/NiO showed higher catalytic potential compared to dopant-free NiO, while Cu-doped CNC/NiO nanostructures exhibited significant potential for use in industrial dye degradation applications.
34920059	6	45	theme	antibacterial	932:944	arg1	activity					946:953	good antibacterial activity	927:953	good antibacterial activity against Escherichia coli (E. coli) bacteria and its bacterial activity	927:1024	Besides this, CNC/NiO composite showed good antibacterial activity against Escherichia coli (E. coli) bacteria and its bacterial activity increased with Cu doping.
34920059	3	46	theme	rhombohedral	374:385	arg1	structure					387:395	rhombohedral structure	374:395	rhombohedral structure of synthesized nanostructures	374:425	XRD patterns confirmed rhombohedral structure of synthesized nanostructures with gradual increase in crystallite size with doping.
34920059	7	47	theme	dihydrofolate	1103:1115	arg1	reductase					1117:1125	dihydrofolate reductase	1103:1125	dihydrofolate reductase	1103:1125	Furthermore, molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme confirmed interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets and showed good agreement with in vitro bactericidal activity.
34920059	3	48	theme	nanostructures	412:425	arg1	structure					387:395	rhombohedral structure	374:395	rhombohedral structure of synthesized nanostructures	374:425	XRD patterns confirmed rhombohedral structure of synthesized nanostructures with gradual increase in crystallite size with doping.
34920059	5	49	theme	catalytic	717:725	arg1	potential					727:735	higher catalytic potential	710:735	higher catalytic potential	710:735	Both CNC/NiO composite and Cu-doped CNC/NiO showed higher catalytic potential compared to dopant-free NiO, while Cu-doped CNC/NiO nanostructures exhibited significant potential for use in industrial dye degradation applications.
34920059	0	50	theme	Cu-doped	63:70	arg1	NiO					72:74	catalytic Cu-doped NiO	53:74	catalytic Cu-doped NiO	53:74	Bactericidal action and molecular docking studies of catalytic Cu-doped NiO composited with cellulose nanocrystals.
34920059	7	51	theme	reductase	1117:1125	arg1	enzyme					1142:1147	dihydrofolate reductase and DNA gyrase enzyme	1103:1147	dihydrofolate reductase and DNA gyrase enzyme	1103:1147	Furthermore, molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme confirmed interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets and showed good agreement with in vitro bactericidal activity.
34920059	2	52	theme	samples	342:348	arg1	studies					322:328	the catalytic, antibacterial and molecular docking studies	271:328	the catalytic, antibacterial and molecular docking studies of prepared samples	271:348	The aim of this study is to investigate the catalytic, antibacterial and molecular docking studies of prepared samples.
34920059	5	53	theme	degradation	862:872	arg1	applications					874:885	industrial dye degradation applications	847:885	industrial dye degradation applications	847:885	Both CNC/NiO composite and Cu-doped CNC/NiO showed higher catalytic potential compared to dopant-free NiO, while Cu-doped CNC/NiO nanostructures exhibited significant potential for use in industrial dye degradation applications.
34920059	4	54	theme	groups	569:574	arg1	presence					576:583	functional groups presence	558:583	functional groups presence in dopant-free and doped nanostructures	558:623	The morphology as well as interlayer spacing was evaluated with HRTEM while functional groups presence in dopant-free and doped nanostructures was confirmed using FTIR spectra.
34920059	1	55	theme	Cu-doped	129:136	arg1	NiO					138:140	Cu-doped NiO	129:140	Cu-doped NiO composited with cellulose nanocrystals (CNC)	129:185	Synthesis of Cu-doped NiO composited with cellulose nanocrystals (CNC) was carried out by co-precipitation method.
34920059	4	56	theme	functional	558:567	arg1	presence					576:583	functional groups presence	558:583	functional groups presence in dopant-free and doped nanostructures	558:623	The morphology as well as interlayer spacing was evaluated with HRTEM while functional groups presence in dopant-free and doped nanostructures was confirmed using FTIR spectra.
34920059	3	57	with	size	464:467	arg1	doping					474:479	doping	474:479	doping	474:479	XRD patterns confirmed rhombohedral structure of synthesized nanostructures with gradual increase in crystallite size with doping.
34920059	5	58	from	use	840:842	arg1	applications					874:885	industrial dye degradation applications	847:885	industrial dye degradation applications	847:885	Both CNC/NiO composite and Cu-doped CNC/NiO showed higher catalytic potential compared to dopant-free NiO, while Cu-doped CNC/NiO nanostructures exhibited significant potential for use in industrial dye degradation applications.
34920059	1	59	theme	NiO	138:140	arg1	Synthesis					116:124	Synthesis	116:124	Synthesis of Cu-doped NiO composited with cellulose nanocrystals (CNC)	116:185	Synthesis of Cu-doped NiO composited with cellulose nanocrystals (CNC) was carried out by co-precipitation method.
34920059	4	60	theme	FTIR	645:648	arg1	spectra					650:656	FTIR spectra	645:656	FTIR spectra	645:656	The morphology as well as interlayer spacing was evaluated with HRTEM while functional groups presence in dopant-free and doped nanostructures was confirmed using FTIR spectra.
34920059	7	61	theme	DNA	1131:1133	arg1	gyrase					1135:1140	DNA gyrase	1131:1140	DNA gyrase	1131:1140	Furthermore, molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme confirmed interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets and showed good agreement with in vitro bactericidal activity.
34920059	6	62	theme	CNC/NiO	902:908	arg1	composite					910:918	CNC/NiO composite	902:918	CNC/NiO composite	902:918	Besides this, CNC/NiO composite showed good antibacterial activity against Escherichia coli (E. coli) bacteria and its bacterial activity increased with Cu doping.
34920059	7	63	theme	molecular	1065:1073	arg1	predictions					1083:1093	molecular docking predictions	1065:1093	molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme	1065:1147	Furthermore, molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme confirmed interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets and showed good agreement with in vitro bactericidal activity.
34920059	6	64	dep	Escherichia	963:973	arg1	coli					975:978	coli	975:978	coli	975:978	Besides this, CNC/NiO composite showed good antibacterial activity against Escherichia coli (E. coli) bacteria and its bacterial activity increased with Cu doping.
34920059	0	65	theme	NiO	72:74	arg1	studies					42:48	molecular docking studies	24:48	molecular docking studies	24:48	Bactericidal action and molecular docking studies of catalytic Cu-doped NiO composited with cellulose nanocrystals.
34920059	0	65	theme	NiO	72:74	arg1	action					13:18	Bactericidal action	0:18	Bactericidal action	0:18	Bactericidal action and molecular docking studies of catalytic Cu-doped NiO composited with cellulose nanocrystals.
34920059	2	66	theme	study	247:251	arg1	aim					235:237	The aim	231:237	The aim of this study	231:251	The aim of this study is to investigate the catalytic, antibacterial and molecular docking studies of prepared samples.
34920059	7	67	theme	docking	1075:1081	arg1	predictions					1083:1093	molecular docking predictions	1065:1093	molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme	1065:1147	Furthermore, molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme confirmed interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets and showed good agreement with in vitro bactericidal activity.
34920059	0	68	theme	catalytic	53:61	arg1	NiO					72:74	catalytic Cu-doped NiO	53:74	catalytic Cu-doped NiO	53:74	Bactericidal action and molecular docking studies of catalytic Cu-doped NiO composited with cellulose nanocrystals.
34920059	7	69	with	agreement	1250:1258	arg1	activity					1287:1294	in vitro bactericidal activity	1265:1294	in vitro bactericidal activity	1265:1294	Furthermore, molecular docking predictions against dihydrofolate reductase and DNA gyrase enzyme confirmed interaction of NiO NPs, CNC/NiO and Cu-doped CNC/NiO inside active pockets and showed good agreement with in vitro bactericidal activity.
34920059	1	70	theme	cellulose	158:166	arg1	CNC					182:184	CNC	182:184	CNC	182:184	Synthesis of Cu-doped NiO composited with cellulose nanocrystals (CNC) was carried out by co-precipitation method.
34920059	1	70	theme	cellulose	158:166	arg1	nanocrystals					168:179	cellulose nanocrystals	158:179	cellulose nanocrystals (CNC)	158:185	Synthesis of Cu-doped NiO composited with cellulose nanocrystals (CNC) was carried out by co-precipitation method.
34920059	5	71	theme	CNC/NiO	781:787	arg1	nanostructures					789:802	Cu-doped CNC/NiO nanostructures	772:802	Cu-doped CNC/NiO nanostructures	772:802	Both CNC/NiO composite and Cu-doped CNC/NiO showed higher catalytic potential compared to dopant-free NiO, while Cu-doped CNC/NiO nanostructures exhibited significant potential for use in industrial dye degradation applications.
34398664	10	0	theme	resistance	1596:1605	arg1	rates					1577:1581	the highest reported rates	1556:1581	the highest reported rates of multidrug resistance among Gram-negative pathogens	1556:1635	IMPORTANCE The opportunistic human pathogen Acinetobacter baumannii displays the highest reported rates of multidrug resistance among Gram-negative pathogens.
34398664	2	1	theme	conjugative	344:354	arg1	plasmids					356:363	large conjugative plasmids	338:363	large conjugative plasmids (LCPs)	338:370	The recent MDR urinary isolate UPAB1 carries the plasmid pAB5, a member of a family of large conjugative plasmids (LCPs).
34398664	2	1	theme	conjugative	344:354	arg1	LCPs					366:369	LCPs	366:369	LCPs	366:369	The recent MDR urinary isolate UPAB1 carries the plasmid pAB5, a member of a family of large conjugative plasmids (LCPs).
34398664	8	2	theme	gene	1185:1188	arg1	cluster					1190:1196	this gene cluster	1180:1196	this gene cluster	1180:1196	Although this gene cluster is widely distributed in Gram-negative bacteria, it remains largely uninvestigated.
34398664	6	3	theme	protein	922:928	arg1	family					930:935	this protein family	917:935	this protein family	917:935	Members of this protein family are involved in amyloid or polysaccharide formation in other species.
34398664	7	4	theme	subsequent	1128:1137	arg1	reduction					1139:1147	a subsequent reduction	1126:1147	a subsequent reduction in biofilm formation	1126:1168	Deletion of the CsgG homolog abrogated PNAG production and chaperone-usher pathway (CUP) pilus formation, resulting in a subsequent reduction in biofilm formation.
34398664	2	5	theme	plasmid	300:306	arg1	pAB5					308:311	the plasmid pAB5	296:311	the plasmid pAB5	296:311	The recent MDR urinary isolate UPAB1 carries the plasmid pAB5, a member of a family of large conjugative plasmids (LCPs).
34398664	2	5	theme	plasmid	300:306	arg1	member					316:321	a member	314:321	a member of a family of large conjugative plasmids (LCPs)	314:370	The recent MDR urinary isolate UPAB1 carries the plasmid pAB5, a member of a family of large conjugative plasmids (LCPs).
34398664	11	6	theme	A.	1643:1644	arg1	strains					1656:1662	Many A. baumannii strains	1638:1662	Many A. baumannii strains	1638:1662	Many A. baumannii strains carry large conjugative plasmids like pAB5.
34398664	3	7	theme	TetR	514:517	arg1	regulators					535:544	two TetR transcriptional regulators	510:544	two TetR transcriptional regulators	510:544	LCPs encode several antibiotic resistance genes and repress the type VI secretion system (T6SS) to enable their dissemination, employing two TetR transcriptional regulators.
34398664	12	8	dep	regulatory	1778:1787	arg1	cross-talks					1789:1799	cross-talks	1789:1799	cross-talks	1789:1799	In recent years, we have witnessed an increase in knowledge about the regulatory cross-talks between plasmids and bacterial chromosomes.
34398664	8	9	theme	Gram-negative	1223:1235	arg1	bacteria					1237:1244	Gram-negative bacteria	1223:1244	Gram-negative bacteria	1223:1244	Although this gene cluster is widely distributed in Gram-negative bacteria, it remains largely uninvestigated.
34398664	6	10	theme	other	992:996	arg1	species					998:1004	other species	992:1004	other species	992:1004	Members of this protein family are involved in amyloid or polysaccharide formation in other species.
34398664	12	11	theme	bacterial	1822:1830	arg1	chromosomes					1832:1842	bacterial chromosomes	1822:1842	bacterial chromosomes	1822:1842	In recent years, we have witnessed an increase in knowledge about the regulatory cross-talks between plasmids and bacterial chromosomes.
34398664	7	12	theme	pilus	1096:1100	arg1	formation					1102:1110	chaperone-usher pathway (CUP) pilus formation	1066:1110	chaperone-usher pathway (CUP) pilus formation	1066:1110	Deletion of the CsgG homolog abrogated PNAG production and chaperone-usher pathway (CUP) pilus formation, resulting in a subsequent reduction in biofilm formation.
34398664	7	13	theme	CUP	1091:1093	arg1	formation					1102:1110	chaperone-usher pathway (CUP) pilus formation	1066:1110	chaperone-usher pathway (CUP) pilus formation	1066:1110	Deletion of the CsgG homolog abrogated PNAG production and chaperone-usher pathway (CUP) pilus formation, resulting in a subsequent reduction in biofilm formation.
34398664	5	14	theme	cluster	840:846	arg1	expression					786:795	the expression	782:795	the expression of a previously uncharacterized three-gene cluster that encodes a protein belonging to the CsgG/HfaB family	782:903	Here, we show that a pAB5-encoded H-NS transcriptional regulator represses the synthesis of the exopolysaccharide PNAG and the expression of a previously uncharacterized three-gene cluster that encodes a protein belonging to the CsgG/HfaB family.
34398664	5	14	theme	cluster	840:846	arg1	synthesis					738:746	the synthesis	734:746	the synthesis of the exopolysaccharide PNAG	734:776	Here, we show that a pAB5-encoded H-NS transcriptional regulator represses the synthesis of the exopolysaccharide PNAG and the expression of a previously uncharacterized three-gene cluster that encodes a protein belonging to the CsgG/HfaB family.
34398664	1	15	theme	health	233:238	arg1	worldwide					240:248	human health worldwide	227:248	human health worldwide	227:248	Acinetobacter baumannii is emerging as a multidrug-resistant (MDR) nosocomial pathogen of increasing threat to human health worldwide.
34398664	7	16	theme	pathway	1082:1088	arg1	formation					1102:1110	chaperone-usher pathway (CUP) pilus formation	1066:1110	chaperone-usher pathway (CUP) pilus formation	1066:1110	Deletion of the CsgG homolog abrogated PNAG production and chaperone-usher pathway (CUP) pilus formation, resulting in a subsequent reduction in biofilm formation.
34398664	7	17	theme	CsgG	1023:1026	arg1	homolog					1028:1034	the CsgG homolog	1019:1034	the CsgG homolog	1019:1034	Deletion of the CsgG homolog abrogated PNAG production and chaperone-usher pathway (CUP) pilus formation, resulting in a subsequent reduction in biofilm formation.
34398664	0	18	dep	Acinetobacter	75:87	arg1	baumannii					89:97	baumannii	89:97	baumannii	89:97	Plasmid-Encoded H-NS Controls Extracellular Matrix Composition in a Modern Acinetobacter baumannii Urinary Isolate.
34398664	5	19	theme	pAB5-encoded	680:691	arg1	regulator					714:722	a pAB5-encoded H-NS transcriptional regulator	678:722	a pAB5-encoded H-NS transcriptional regulator	678:722	Here, we show that a pAB5-encoded H-NS transcriptional regulator represses the synthesis of the exopolysaccharide PNAG and the expression of a previously uncharacterized three-gene cluster that encodes a protein belonging to the CsgG/HfaB family.
34398664	2	20	theme	isolate	274:280	arg1	UPAB1					282:286	The recent MDR urinary isolate UPAB1	251:286	The recent MDR urinary isolate UPAB1	251:286	The recent MDR urinary isolate UPAB1 carries the plasmid pAB5, a member of a family of large conjugative plasmids (LCPs).
34398664	5	21	theme	PNAG	773:776	arg1	expression					786:795	the expression	782:795	the expression of a previously uncharacterized three-gene cluster that encodes a protein belonging to the CsgG/HfaB family	782:903	Here, we show that a pAB5-encoded H-NS transcriptional regulator represses the synthesis of the exopolysaccharide PNAG and the expression of a previously uncharacterized three-gene cluster that encodes a protein belonging to the CsgG/HfaB family.
34398664	5	21	theme	PNAG	773:776	arg1	synthesis					738:746	the synthesis	734:746	the synthesis of the exopolysaccharide PNAG	734:776	Here, we show that a pAB5-encoded H-NS transcriptional regulator represses the synthesis of the exopolysaccharide PNAG and the expression of a previously uncharacterized three-gene cluster that encodes a protein belonging to the CsgG/HfaB family.
34398664	2	22	theme	MDR	262:264	arg1	UPAB1					282:286	The recent MDR urinary isolate UPAB1	251:286	The recent MDR urinary isolate UPAB1	251:286	The recent MDR urinary isolate UPAB1 carries the plasmid pAB5, a member of a family of large conjugative plasmids (LCPs).
34398664	15	23	theme	formation	2163:2171	arg1	regulation					2141:2150	the regulation	2137:2150	the regulation of biofilm formation	2137:2171	Therefore, understanding the factors involved in the regulation of biofilm formation in Acinetobacter has remarkable therapeutic potential.
34398664	10	24	theme	human	1508:1512	arg1	pathogen					1514:1521	The opportunistic human pathogen	1490:1521	IMPORTANCE The opportunistic human pathogen Acinetobacter baumannii	1479:1545	IMPORTANCE The opportunistic human pathogen Acinetobacter baumannii displays the highest reported rates of multidrug resistance among Gram-negative pathogens.
34398664	0	25	theme	Acinetobacter	75:87	arg1	Isolate					107:113	a Modern Acinetobacter baumannii Urinary Isolate	66:113	a Modern Acinetobacter baumannii Urinary Isolate	66:113	Plasmid-Encoded H-NS Controls Extracellular Matrix Composition in a Modern Acinetobacter baumannii Urinary Isolate.
34398664	13	26	theme	drastic	1948:1954	arg1	reduction					1956:1964	a drastic reduction	1946:1964	a drastic reduction in biofilm formation	1946:1985	Here, we show that pAB5 controls the composition of the bacterial extracellular matrix, resulting in a drastic reduction in biofilm formation.
34398664	0	27	theme	Plasmid-Encoded	0:14	arg1	H-NS					16:19	Plasmid-Encoded H-NS	0:19	Plasmid-Encoded H-NS	0:19	Plasmid-Encoded H-NS Controls Extracellular Matrix Composition in a Modern Acinetobacter baumannii Urinary Isolate.
34398664	2	28	contain	carries	288:294	arg2	member					316:321	a member	314:321	a member of a family of large conjugative plasmids (LCPs)	314:370	The recent MDR urinary isolate UPAB1 carries the plasmid pAB5, a member of a family of large conjugative plasmids (LCPs).
34398664	2	28	contain	carries	288:294	arg2	pAB5					308:311	the plasmid pAB5	296:311	the plasmid pAB5	296:311	The recent MDR urinary isolate UPAB1 carries the plasmid pAB5, a member of a family of large conjugative plasmids (LCPs).
34398664	2	28	contain	carries	288:294	arg1	UPAB1					282:286	The recent MDR urinary isolate UPAB1	251:286	The recent MDR urinary isolate UPAB1	251:286	The recent MDR urinary isolate UPAB1 carries the plasmid pAB5, a member of a family of large conjugative plasmids (LCPs).
34398664	12	29	theme	recent	1711:1716	arg1	years					1718:1722	recent years	1711:1722	recent years	1711:1722	In recent years, we have witnessed an increase in knowledge about the regulatory cross-talks between plasmids and bacterial chromosomes.
34398664	10	30	theme	highest	1560:1566	arg1	rates					1577:1581	the highest reported rates	1556:1581	the highest reported rates of multidrug resistance among Gram-negative pathogens	1556:1635	IMPORTANCE The opportunistic human pathogen Acinetobacter baumannii displays the highest reported rates of multidrug resistance among Gram-negative pathogens.
34398664	13	31	theme	extracellular	1911:1923	arg1	matrix					1925:1930	the bacterial extracellular matrix	1897:1930	the bacterial extracellular matrix	1897:1930	Here, we show that pAB5 controls the composition of the bacterial extracellular matrix, resulting in a drastic reduction in biofilm formation.
34398664	10	32	dep	pathogen	1514:1521	arg1	baumannii					1537:1545	Acinetobacter baumannii	1523:1545	IMPORTANCE The opportunistic human pathogen Acinetobacter baumannii	1479:1545	IMPORTANCE The opportunistic human pathogen Acinetobacter baumannii displays the highest reported rates of multidrug resistance among Gram-negative pathogens.
34398664	3	33	theme	resistance	404:413	arg1	LCPs					373:376	LCPs	373:376	LCPs	373:376	LCPs encode several antibiotic resistance genes and repress the type VI secretion system (T6SS) to enable their dissemination, employing two TetR transcriptional regulators.
34398664	3	33	theme	resistance	404:413	arg1	genes					415:419	several antibiotic resistance genes	385:419	several antibiotic resistance genes	385:419	LCPs encode several antibiotic resistance genes and repress the type VI secretion system (T6SS) to enable their dissemination, employing two TetR transcriptional regulators.
34398664	0	34	theme	Matrix	44:49	arg1	Composition					51:61	Extracellular Matrix Composition	30:61	Extracellular Matrix Composition	30:61	Plasmid-Encoded H-NS Controls Extracellular Matrix Composition in a Modern Acinetobacter baumannii Urinary Isolate.
34398664	9	35	theme	host	1401:1404	arg1	plasmids					1353:1360	plasmids	1353:1360	plasmids	1353:1360	Our results illustrate the complex cross-talks that take place between plasmids and the chromosomes of their bacterial host, which in this case can contribute to the pathogenesis of Acinetobacter.
34398664	9	35	theme	host	1401:1404	arg1	chromosomes					1370:1380	the chromosomes	1366:1380	the chromosomes of their bacterial host, which in this case can contribute to the pathogenesis of Acinetobacter	1366:1476	Our results illustrate the complex cross-talks that take place between plasmids and the chromosomes of their bacterial host, which in this case can contribute to the pathogenesis of Acinetobacter.
34398664	14	36	theme	antibiotic	2046:2055	arg1	resistance					2057:2066	antibiotic resistance	2046:2066	antibiotic resistance	2046:2066	The association between biofilm formation, virulence, and antibiotic resistance is well documented.
34398664	3	37	theme	several	385:391	arg1	LCPs					373:376	LCPs	373:376	LCPs	373:376	LCPs encode several antibiotic resistance genes and repress the type VI secretion system (T6SS) to enable their dissemination, employing two TetR transcriptional regulators.
34398664	3	37	theme	several	385:391	arg1	genes					415:419	several antibiotic resistance genes	385:419	several antibiotic resistance genes	385:419	LCPs encode several antibiotic resistance genes and repress the type VI secretion system (T6SS) to enable their dissemination, employing two TetR transcriptional regulators.
34398664	10	38	theme	multidrug	1586:1594	arg1	resistance					1596:1605	multidrug resistance	1586:1605	multidrug resistance among Gram-negative pathogens	1586:1635	IMPORTANCE The opportunistic human pathogen Acinetobacter baumannii displays the highest reported rates of multidrug resistance among Gram-negative pathogens.
34398664	0	39	theme	Modern	68:73	arg1	Isolate					107:113	a Modern Acinetobacter baumannii Urinary Isolate	66:113	a Modern Acinetobacter baumannii Urinary Isolate	66:113	Plasmid-Encoded H-NS Controls Extracellular Matrix Composition in a Modern Acinetobacter baumannii Urinary Isolate.
34398664	11	40	theme	conjugative	1676:1686	arg1	plasmids					1688:1695	large conjugative plasmids	1670:1695	large conjugative plasmids	1670:1695	Many A. baumannii strains carry large conjugative plasmids like pAB5.
34398664	2	41	theme	large	338:342	arg1	plasmids					356:363	large conjugative plasmids	338:363	large conjugative plasmids (LCPs)	338:370	The recent MDR urinary isolate UPAB1 carries the plasmid pAB5, a member of a family of large conjugative plasmids (LCPs).
34398664	2	41	theme	large	338:342	arg1	LCPs					366:369	LCPs	366:369	LCPs	366:369	The recent MDR urinary isolate UPAB1 carries the plasmid pAB5, a member of a family of large conjugative plasmids (LCPs).
34398664	10	42	dep	IMPORTANCE	1479:1488	arg1	pathogen					1514:1521	The opportunistic human pathogen	1490:1521	IMPORTANCE The opportunistic human pathogen Acinetobacter baumannii	1479:1545	IMPORTANCE The opportunistic human pathogen Acinetobacter baumannii displays the highest reported rates of multidrug resistance among Gram-negative pathogens.
34398664	9	43	theme	Acinetobacter	1464:1476	arg1	pathogenesis					1448:1459	the pathogenesis	1444:1459	the pathogenesis of Acinetobacter	1444:1476	Our results illustrate the complex cross-talks that take place between plasmids and the chromosomes of their bacterial host, which in this case can contribute to the pathogenesis of Acinetobacter.
34398664	2	44	theme	family	328:333	arg1	pAB5					308:311	the plasmid pAB5	296:311	the plasmid pAB5	296:311	The recent MDR urinary isolate UPAB1 carries the plasmid pAB5, a member of a family of large conjugative plasmids (LCPs).
34398664	2	44	theme	family	328:333	arg1	member					316:321	a member	314:321	a member of a family of large conjugative plasmids (LCPs)	314:370	The recent MDR urinary isolate UPAB1 carries the plasmid pAB5, a member of a family of large conjugative plasmids (LCPs).
34398664	15	45	theme	remarkable	2194:2203	arg1	potential					2217:2225	remarkable therapeutic potential	2194:2225	remarkable therapeutic potential	2194:2225	Therefore, understanding the factors involved in the regulation of biofilm formation in Acinetobacter has remarkable therapeutic potential.
34398664	4	46	theme	additional	592:601	arg1	genes					625:629	additional chromosomally encoded genes	592:629	additional chromosomally encoded genes	592:629	Furthermore, pAB5 controls the expression of additional chromosomally encoded genes, impacting UPAB1 virulence.
34398664	14	47	theme	biofilm	2012:2018	arg1	formation					2020:2028	biofilm formation	2012:2028	biofilm formation	2012:2028	The association between biofilm formation, virulence, and antibiotic resistance is well documented.
34398664	4	48	theme	encoded	617:623	arg1	genes					625:629	additional chromosomally encoded genes	592:629	additional chromosomally encoded genes	592:629	Furthermore, pAB5 controls the expression of additional chromosomally encoded genes, impacting UPAB1 virulence.
34398664	2	49	theme	plasmids	356:363	arg1	family					328:333	a family	326:333	a family of large conjugative plasmids (LCPs)	326:370	The recent MDR urinary isolate UPAB1 carries the plasmid pAB5, a member of a family of large conjugative plasmids (LCPs).
34398664	6	50	theme	family	930:935	arg1	Members					906:912	Members	906:912	Members of this protein family	906:935	Members of this protein family are involved in amyloid or polysaccharide formation in other species.
34398664	11	51	dep	A.	1643:1644	arg1	baumannii					1646:1654	baumannii	1646:1654	baumannii	1646:1654	Many A. baumannii strains carry large conjugative plasmids like pAB5.
34398664	9	52	dep	complex	1309:1315	arg1	cross-talks					1317:1327	cross-talks	1317:1327	cross-talks	1317:1327	Our results illustrate the complex cross-talks that take place between plasmids and the chromosomes of their bacterial host, which in this case can contribute to the pathogenesis of Acinetobacter.
34398664	3	53	theme	transcriptional	519:533	arg1	regulators					535:544	two TetR transcriptional regulators	510:544	two TetR transcriptional regulators	510:544	LCPs encode several antibiotic resistance genes and repress the type VI secretion system (T6SS) to enable their dissemination, employing two TetR transcriptional regulators.
34398664	1	54	theme	nosocomial	183:192	arg1	pathogen					194:201	a multidrug-resistant (MDR) nosocomial pathogen	155:201	a multidrug-resistant (MDR) nosocomial pathogen of increasing threat to human health worldwide	155:248	Acinetobacter baumannii is emerging as a multidrug-resistant (MDR) nosocomial pathogen of increasing threat to human health worldwide.
34398664	5	55	theme	CsgG/HfaB	888:896	arg1	family					898:903	the CsgG/HfaB family	884:903	the CsgG/HfaB family	884:903	Here, we show that a pAB5-encoded H-NS transcriptional regulator represses the synthesis of the exopolysaccharide PNAG and the expression of a previously uncharacterized three-gene cluster that encodes a protein belonging to the CsgG/HfaB family.
34398664	5	56	theme	H-NS	693:696	arg1	regulator					714:722	a pAB5-encoded H-NS transcriptional regulator	678:722	a pAB5-encoded H-NS transcriptional regulator	678:722	Here, we show that a pAB5-encoded H-NS transcriptional regulator represses the synthesis of the exopolysaccharide PNAG and the expression of a previously uncharacterized three-gene cluster that encodes a protein belonging to the CsgG/HfaB family.
34398664	11	57	theme	Many	1638:1641	arg1	strains					1656:1662	Many A. baumannii strains	1638:1662	Many A. baumannii strains	1638:1662	Many A. baumannii strains carry large conjugative plasmids like pAB5.
34398664	2	58	theme	recent	255:260	arg1	UPAB1					282:286	The recent MDR urinary isolate UPAB1	251:286	The recent MDR urinary isolate UPAB1	251:286	The recent MDR urinary isolate UPAB1 carries the plasmid pAB5, a member of a family of large conjugative plasmids (LCPs).
34398664	1	59	theme	human	227:231	arg1	worldwide					240:248	human health worldwide	227:248	human health worldwide	227:248	Acinetobacter baumannii is emerging as a multidrug-resistant (MDR) nosocomial pathogen of increasing threat to human health worldwide.
34398664	6	60	theme	polysaccharide	964:977	arg1	formation					979:987	amyloid or polysaccharide formation	953:987	amyloid or polysaccharide formation in other species	953:1004	Members of this protein family are involved in amyloid or polysaccharide formation in other species.
34398664	4	61	theme	UPAB1	642:646	arg1	virulence					648:656	UPAB1 virulence	642:656	UPAB1 virulence	642:656	Furthermore, pAB5 controls the expression of additional chromosomally encoded genes, impacting UPAB1 virulence.
34398664	7	62	theme	chaperone-usher	1066:1080	arg1	formation					1102:1110	chaperone-usher pathway (CUP) pilus formation	1066:1110	chaperone-usher pathway (CUP) pilus formation	1066:1110	Deletion of the CsgG homolog abrogated PNAG production and chaperone-usher pathway (CUP) pilus formation, resulting in a subsequent reduction in biofilm formation.
34398664	7	63	theme	PNAG	1046:1049	arg1	production					1051:1060	PNAG production	1046:1060	PNAG production	1046:1060	Deletion of the CsgG homolog abrogated PNAG production and chaperone-usher pathway (CUP) pilus formation, resulting in a subsequent reduction in biofilm formation.
34398664	6	64	theme	amyloid	953:959	arg1	formation					979:987	amyloid or polysaccharide formation	953:987	amyloid or polysaccharide formation in other species	953:1004	Members of this protein family are involved in amyloid or polysaccharide formation in other species.
34398664	7	65	theme	homolog	1028:1034	arg1	Deletion					1007:1014	Deletion	1007:1014	Deletion of the CsgG homolog	1007:1034	Deletion of the CsgG homolog abrogated PNAG production and chaperone-usher pathway (CUP) pilus formation, resulting in a subsequent reduction in biofilm formation.
34398664	5	66	theme	exopolysaccharide	755:771	arg1	PNAG					773:776	the exopolysaccharide PNAG	751:776	the exopolysaccharide PNAG	751:776	Here, we show that a pAB5-encoded H-NS transcriptional regulator represses the synthesis of the exopolysaccharide PNAG and the expression of a previously uncharacterized three-gene cluster that encodes a protein belonging to the CsgG/HfaB family.
34398664	2	67	theme	urinary	266:272	arg1	UPAB1					282:286	The recent MDR urinary isolate UPAB1	251:286	The recent MDR urinary isolate UPAB1	251:286	The recent MDR urinary isolate UPAB1 carries the plasmid pAB5, a member of a family of large conjugative plasmids (LCPs).
34398664	1	68	theme	multidrug-resistant	157:175	arg1	pathogen					194:201	a multidrug-resistant (MDR) nosocomial pathogen	155:201	a multidrug-resistant (MDR) nosocomial pathogen of increasing threat to human health worldwide	155:248	Acinetobacter baumannii is emerging as a multidrug-resistant (MDR) nosocomial pathogen of increasing threat to human health worldwide.
34398664	12	69	from	increase	1746:1753	arg1	knowledge					1758:1766	knowledge	1758:1766	knowledge about the regulatory cross-talks between plasmids and bacterial chromosomes	1758:1842	In recent years, we have witnessed an increase in knowledge about the regulatory cross-talks between plasmids and bacterial chromosomes.
34398664	0	70	theme	Urinary	99:105	arg1	Isolate					107:113	a Modern Acinetobacter baumannii Urinary Isolate	66:113	a Modern Acinetobacter baumannii Urinary Isolate	66:113	Plasmid-Encoded H-NS Controls Extracellular Matrix Composition in a Modern Acinetobacter baumannii Urinary Isolate.
34398664	1	71	theme	MDR	178:180	arg1	pathogen					194:201	a multidrug-resistant (MDR) nosocomial pathogen	155:201	a multidrug-resistant (MDR) nosocomial pathogen of increasing threat to human health worldwide	155:248	Acinetobacter baumannii is emerging as a multidrug-resistant (MDR) nosocomial pathogen of increasing threat to human health worldwide.
34398664	5	72	theme	transcriptional	698:712	arg1	regulator					714:722	a pAB5-encoded H-NS transcriptional regulator	678:722	a pAB5-encoded H-NS transcriptional regulator	678:722	Here, we show that a pAB5-encoded H-NS transcriptional regulator represses the synthesis of the exopolysaccharide PNAG and the expression of a previously uncharacterized three-gene cluster that encodes a protein belonging to the CsgG/HfaB family.
34398664	13	73	theme	biofilm	1969:1975	arg1	formation					1977:1985	biofilm formation	1969:1985	biofilm formation	1969:1985	Here, we show that pAB5 controls the composition of the bacterial extracellular matrix, resulting in a drastic reduction in biofilm formation.
34398664	15	74	theme	biofilm	2155:2161	arg1	formation					2163:2171	biofilm formation	2155:2171	biofilm formation	2155:2171	Therefore, understanding the factors involved in the regulation of biofilm formation in Acinetobacter has remarkable therapeutic potential.
34398664	13	75	from	reduction	1956:1964	arg1	formation					1977:1985	biofilm formation	1969:1985	biofilm formation	1969:1985	Here, we show that pAB5 controls the composition of the bacterial extracellular matrix, resulting in a drastic reduction in biofilm formation.
34398664	3	76	theme	type	437:440	arg1	T6SS					463:466	T6SS	463:466	T6SS	463:466	LCPs encode several antibiotic resistance genes and repress the type VI secretion system (T6SS) to enable their dissemination, employing two TetR transcriptional regulators.
34398664	3	76	theme	type	437:440	arg1	system					455:460	the type VI secretion system	433:460	the type VI secretion system (T6SS) to enable their dissemination	433:497	LCPs encode several antibiotic resistance genes and repress the type VI secretion system (T6SS) to enable their dissemination, employing two TetR transcriptional regulators.
34398664	5	77	theme	uncharacterized	813:827	arg1	protein					863:869	a protein	861:869	a protein belonging to the CsgG/HfaB family	861:903	Here, we show that a pAB5-encoded H-NS transcriptional regulator represses the synthesis of the exopolysaccharide PNAG and the expression of a previously uncharacterized three-gene cluster that encodes a protein belonging to the CsgG/HfaB family.
34398664	5	77	theme	uncharacterized	813:827	arg1	cluster					840:846	a previously uncharacterized three-gene cluster	800:846	a previously uncharacterized three-gene cluster that encodes a protein belonging to the CsgG/HfaB family	800:903	Here, we show that a pAB5-encoded H-NS transcriptional regulator represses the synthesis of the exopolysaccharide PNAG and the expression of a previously uncharacterized three-gene cluster that encodes a protein belonging to the CsgG/HfaB family.
34398664	10	78	theme	opportunistic	1494:1506	arg1	pathogen					1514:1521	The opportunistic human pathogen	1490:1521	IMPORTANCE The opportunistic human pathogen Acinetobacter baumannii	1479:1545	IMPORTANCE The opportunistic human pathogen Acinetobacter baumannii displays the highest reported rates of multidrug resistance among Gram-negative pathogens.
34398664	13	79	theme	bacterial	1901:1909	arg1	matrix					1925:1930	the bacterial extracellular matrix	1897:1930	the bacterial extracellular matrix	1897:1930	Here, we show that pAB5 controls the composition of the bacterial extracellular matrix, resulting in a drastic reduction in biofilm formation.
34398664	0	80	theme	Extracellular	30:42	arg1	Composition					51:61	Extracellular Matrix Composition	30:61	Extracellular Matrix Composition	30:61	Plasmid-Encoded H-NS Controls Extracellular Matrix Composition in a Modern Acinetobacter baumannii Urinary Isolate.
34398664	5	81	theme	three-gene	829:838	arg1	protein					863:869	a protein	861:869	a protein belonging to the CsgG/HfaB family	861:903	Here, we show that a pAB5-encoded H-NS transcriptional regulator represses the synthesis of the exopolysaccharide PNAG and the expression of a previously uncharacterized three-gene cluster that encodes a protein belonging to the CsgG/HfaB family.
34398664	5	81	theme	three-gene	829:838	arg1	cluster					840:846	a previously uncharacterized three-gene cluster	800:846	a previously uncharacterized three-gene cluster that encodes a protein belonging to the CsgG/HfaB family	800:903	Here, we show that a pAB5-encoded H-NS transcriptional regulator represses the synthesis of the exopolysaccharide PNAG and the expression of a previously uncharacterized three-gene cluster that encodes a protein belonging to the CsgG/HfaB family.
34398664	1	82	theme	Acinetobacter	116:128	arg1	baumannii					130:138	Acinetobacter baumannii	116:138	Acinetobacter baumannii	116:138	Acinetobacter baumannii is emerging as a multidrug-resistant (MDR) nosocomial pathogen of increasing threat to human health worldwide.
34398664	6	83	from	formation	979:987	arg1	species					998:1004	other species	992:1004	other species	992:1004	Members of this protein family are involved in amyloid or polysaccharide formation in other species.
34398664	13	84	theme	matrix	1925:1930	arg1	composition					1882:1892	the composition	1878:1892	the composition of the bacterial extracellular matrix	1878:1930	Here, we show that pAB5 controls the composition of the bacterial extracellular matrix, resulting in a drastic reduction in biofilm formation.
34398664	9	85	theme	bacterial	1391:1399	arg1	host					1401:1404	their bacterial host	1385:1404	their bacterial host	1385:1404	Our results illustrate the complex cross-talks that take place between plasmids and the chromosomes of their bacterial host, which in this case can contribute to the pathogenesis of Acinetobacter.
34398664	11	86	theme	large	1670:1674	arg1	plasmids					1688:1695	large conjugative plasmids	1670:1695	large conjugative plasmids	1670:1695	Many A. baumannii strains carry large conjugative plasmids like pAB5.
34398664	3	87	theme	secretion	445:453	arg1	T6SS					463:466	T6SS	463:466	T6SS	463:466	LCPs encode several antibiotic resistance genes and repress the type VI secretion system (T6SS) to enable their dissemination, employing two TetR transcriptional regulators.
34398664	3	87	theme	secretion	445:453	arg1	system					455:460	the type VI secretion system	433:460	the type VI secretion system (T6SS) to enable their dissemination	433:497	LCPs encode several antibiotic resistance genes and repress the type VI secretion system (T6SS) to enable their dissemination, employing two TetR transcriptional regulators.
34398664	7	88	theme	biofilm	1152:1158	arg1	formation					1160:1168	biofilm formation	1152:1168	biofilm formation	1152:1168	Deletion of the CsgG homolog abrogated PNAG production and chaperone-usher pathway (CUP) pilus formation, resulting in a subsequent reduction in biofilm formation.
34398664	11	89	contain	carry	1664:1668	arg1	strains					1656:1662	Many A. baumannii strains	1638:1662	Many A. baumannii strains	1638:1662	Many A. baumannii strains carry large conjugative plasmids like pAB5.
34398664	11	89	contain	carry	1664:1668	arg2	plasmids					1688:1695	large conjugative plasmids	1670:1695	large conjugative plasmids	1670:1695	Many A. baumannii strains carry large conjugative plasmids like pAB5.
34398664	15	90	theme	therapeutic	2205:2215	arg1	potential					2217:2225	remarkable therapeutic potential	2194:2225	remarkable therapeutic potential	2194:2225	Therefore, understanding the factors involved in the regulation of biofilm formation in Acinetobacter has remarkable therapeutic potential.
34398664	3	91	theme	antibiotic	393:402	arg1	LCPs					373:376	LCPs	373:376	LCPs	373:376	LCPs encode several antibiotic resistance genes and repress the type VI secretion system (T6SS) to enable their dissemination, employing two TetR transcriptional regulators.
34398664	3	91	theme	antibiotic	393:402	arg1	genes					415:419	several antibiotic resistance genes	385:419	several antibiotic resistance genes	385:419	LCPs encode several antibiotic resistance genes and repress the type VI secretion system (T6SS) to enable their dissemination, employing two TetR transcriptional regulators.
34398664	7	92	from	reduction	1139:1147	arg1	formation					1160:1168	biofilm formation	1152:1168	biofilm formation	1152:1168	Deletion of the CsgG homolog abrogated PNAG production and chaperone-usher pathway (CUP) pilus formation, resulting in a subsequent reduction in biofilm formation.
34398664	10	93	theme	reported	1568:1575	arg1	rates					1577:1581	the highest reported rates	1556:1581	the highest reported rates of multidrug resistance among Gram-negative pathogens	1556:1635	IMPORTANCE The opportunistic human pathogen Acinetobacter baumannii displays the highest reported rates of multidrug resistance among Gram-negative pathogens.
34398664	3	94	dep	system	455:460	arg1	VI					442:443	VI	442:443	VI	442:443	LCPs encode several antibiotic resistance genes and repress the type VI secretion system (T6SS) to enable their dissemination, employing two TetR transcriptional regulators.
34398664	4	95	theme	genes	625:629	arg1	expression					578:587	the expression	574:587	the expression of additional chromosomally encoded genes	574:629	Furthermore, pAB5 controls the expression of additional chromosomally encoded genes, impacting UPAB1 virulence.
34398664	10	96	theme	Gram-negative	1613:1625	arg1	pathogens					1627:1635	Gram-negative pathogens	1613:1635	Gram-negative pathogens	1613:1635	IMPORTANCE The opportunistic human pathogen Acinetobacter baumannii displays the highest reported rates of multidrug resistance among Gram-negative pathogens.
33446763	0	0	theme	chloride	72:79	arg1	stresses					81:88	sodium chloride stresses	65:88	sodium chloride stresses	65:88	Response of Lactobacillus plantarum VAL6 to challenges of pH and sodium chloride stresses.
33446763	4	1	theme	plantarum	878:886	arg1	VAL6					888:891	Lactobacillus plantarum VAL6	864:891	Lactobacillus plantarum VAL6	864:891	The galactose content was highest under stress condition of pH 8 meantime rhamnose content increased sharply when Lactobacillus plantarum VAL6 was stressed at pH 3.
33446763	0	2	theme	sodium	65:70	arg1	stresses					81:88	sodium chloride stresses	65:88	sodium chloride stresses	65:88	Response of Lactobacillus plantarum VAL6 to challenges of pH and sodium chloride stresses.
33446763	3	3	theme	sodium	603:608	arg1	chloride					610:617	sodium chloride	603:617	sodium chloride	603:617	Under pH and sodium chloride stresses, the mannose content in exopolysaccharides decreased while the glucose increased in comparison with non-stress condition.
33446763	1	4	theme	exopolysaccharide	150:166	arg1	biosynthesis					168:179	the exopolysaccharide biosynthesis	146:179	the exopolysaccharide biosynthesis	146:179	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	5	5	from	content	929:935	arg1	exopolysaccharides					940:957	exopolysaccharides	940:957	exopolysaccharides	940:957	The arabinose content in exopolysaccharides was not detected under non-stress condition but it was recorded in great amounts after 3 h of stress at pH 3.
33446763	1	6	theme	sodium	237:242	arg1	chloride					244:251	sodium chloride	237:251	sodium chloride	237:251	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	3	7	theme	mannose	633:639	arg1	content					641:647	the mannose content	629:647	the mannose content in exopolysaccharides	629:669	Under pH and sodium chloride stresses, the mannose content in exopolysaccharides decreased while the glucose increased in comparison with non-stress condition.
33446763	5	8	from	pH	1063:1064	arg1	3 h					1046:1048	3 h	1046:1048	3 h of stress at pH 3	1046:1066	The arabinose content in exopolysaccharides was not detected under non-stress condition but it was recorded in great amounts after 3 h of stress at pH 3.
33446763	0	9	theme	stresses	81:88	arg1	challenges					44:53	challenges	44:53	challenges of pH and sodium chloride stresses	44:88	Response of Lactobacillus plantarum VAL6 to challenges of pH and sodium chloride stresses.
33446763	4	10	theme	pH	810:811	arg1	condition					797:805	stress condition	790:805	stress condition of pH 8 meantime rhamnose content increased sharply when Lactobacillus plantarum VAL6 was stressed at pH 3	790:912	The galactose content was highest under stress condition of pH 8 meantime rhamnose content increased sharply when Lactobacillus plantarum VAL6 was stressed at pH 3.
33446763	7	11	theme	chloride	1239:1246	arg1	stresses					1213:1220	the stresses	1209:1220	the stresses of pH and sodium chloride	1209:1246	According to our results, the stresses of pH and sodium chloride enhance the production and change the mRNA expression of epsF gene, leading to differences in the monosaccharide composition of exopolysaccharides.
33446763	6	12	theme	mRNA	1111:1114	arg1	expression					1116:1125	the mRNA expression	1107:1125	the mRNA expression of epsF gene	1107:1138	In addition, stress of pH 8 triggered the mRNA expression of epsF gene resulting in galactose-rich EPS synthesis.
33446763	5	13	theme	stress	1053:1058	arg1	3 h					1046:1048	3 h	1046:1048	3 h of stress at pH 3	1046:1066	The arabinose content in exopolysaccharides was not detected under non-stress condition but it was recorded in great amounts after 3 h of stress at pH 3.
33446763	7	14	theme	exopolysaccharides	1376:1393	arg1	composition					1361:1371	the monosaccharide composition	1342:1371	the monosaccharide composition of exopolysaccharides	1342:1393	According to our results, the stresses of pH and sodium chloride enhance the production and change the mRNA expression of epsF gene, leading to differences in the monosaccharide composition of exopolysaccharides.
33446763	7	15	theme	monosaccharide	1346:1359	arg1	composition					1361:1371	the monosaccharide composition	1342:1371	the monosaccharide composition of exopolysaccharides	1342:1393	According to our results, the stresses of pH and sodium chloride enhance the production and change the mRNA expression of epsF gene, leading to differences in the monosaccharide composition of exopolysaccharides.
33446763	1	16	theme	3	348:348	arg1	pHs					341:343	pHs	341:343	pHs of 3 and 8	341:354	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	4	17	theme	meantime	815:822	arg1	content					833:839	meantime rhamnose content	815:839	meantime rhamnose content	815:839	The galactose content was highest under stress condition of pH 8 meantime rhamnose content increased sharply when Lactobacillus plantarum VAL6 was stressed at pH 3.
33446763	3	18	with	comparison	712:721	arg1	condition					739:747	non-stress condition	728:747	non-stress condition	728:747	Under pH and sodium chloride stresses, the mannose content in exopolysaccharides decreased while the glucose increased in comparison with non-stress condition.
33446763	1	19	theme	Lactobacillus	254:266	arg1	VAL6					278:281	Lactobacillus plantarum VAL6	254:281	Lactobacillus plantarum VAL6	254:281	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	2	20	from	pH	481:482	arg1	stress					471:476	stress	471:476	stress at pH 3	471:484	The results found that Lactobacillus plantarum VAL6 exposed to stress at pH 3 for 3 h gives the highest exopolysaccharide yield (50.44 g/L) which is 6.4 fold higher than non-stress.
33446763	4	21	theme	rhamnose	824:831	arg1	content					833:839	meantime rhamnose content	815:839	meantime rhamnose content	815:839	The galactose content was highest under stress condition of pH 8 meantime rhamnose content increased sharply when Lactobacillus plantarum VAL6 was stressed at pH 3.
33446763	0	22	theme	plantarum	26:34	arg1	VAL6					36:39	Lactobacillus plantarum VAL6	12:39	Lactobacillus plantarum VAL6	12:39	Response of Lactobacillus plantarum VAL6 to challenges of pH and sodium chloride stresses.
33446763	1	23	theme	plantarum	268:276	arg1	VAL6					278:281	Lactobacillus plantarum VAL6	254:281	Lactobacillus plantarum VAL6	254:281	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	2	24	theme	highest	504:510	arg1	yield					530:534	the highest exopolysaccharide yield	500:534	the highest exopolysaccharide yield (50.44 g/L) which is 6.4 fold higher than non-stress	500:587	The results found that Lactobacillus plantarum VAL6 exposed to stress at pH 3 for 3 h gives the highest exopolysaccharide yield (50.44 g/L) which is 6.4 fold higher than non-stress.
33446763	2	24	theme	highest	504:510	arg1	higher					566:571	higher	566:571	higher	566:571	The results found that Lactobacillus plantarum VAL6 exposed to stress at pH 3 for 3 h gives the highest exopolysaccharide yield (50.44 g/L) which is 6.4 fold higher than non-stress.
33446763	2	24	theme	highest	504:510	arg1	50.44 g/L					537:545	50.44 g/L	537:545	50.44 g/L	537:545	The results found that Lactobacillus plantarum VAL6 exposed to stress at pH 3 for 3 h gives the highest exopolysaccharide yield (50.44 g/L) which is 6.4 fold higher than non-stress.
33446763	1	25	from	effect	110:115	arg1	biosynthesis					168:179	the exopolysaccharide biosynthesis	146:179	the exopolysaccharide biosynthesis	146:179	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	6	26	theme	pH	1092:1093	arg1	stress					1082:1087	stress	1082:1087	stress of pH 8	1082:1095	In addition, stress of pH 8 triggered the mRNA expression of epsF gene resulting in galactose-rich EPS synthesis.
33446763	4	27	dep	condition	797:805	arg1	increased					841:849	increased	841:849	increased sharply when Lactobacillus plantarum VAL6 was stressed at pH 3	841:912	The galactose content was highest under stress condition of pH 8 meantime rhamnose content increased sharply when Lactobacillus plantarum VAL6 was stressed at pH 3.
33446763	0	28	theme	Lactobacillus	12:24	arg1	VAL6					36:39	Lactobacillus plantarum VAL6	12:39	Lactobacillus plantarum VAL6	12:39	Response of Lactobacillus plantarum VAL6 to challenges of pH and sodium chloride stresses.
33446763	1	29	with	37 °C	207:211	arg1	pH					218:219	pH 6.8	218:223	pH 6.8	218:223	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	3	30	theme	pH	596:597	arg1	stresses					619:626	pH and sodium chloride stresses	596:626	pH and sodium chloride stresses	596:626	Under pH and sodium chloride stresses, the mannose content in exopolysaccharides decreased while the glucose increased in comparison with non-stress condition.
33446763	5	31	theme	arabinose	919:927	arg1	content					929:935	The arabinose content	915:935	The arabinose content in exopolysaccharides	915:957	The arabinose content in exopolysaccharides was not detected under non-stress condition but it was recorded in great amounts after 3 h of stress at pH 3.
33446763	1	32	theme	culture	196:202	arg1	24 h					188:191	24 h	188:191	24 h of culture at 37 °C with pH 6.8 and without sodium chloride	188:251	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	2	33	theme	plantarum	445:453	arg1	VAL6					455:458	Lactobacillus plantarum VAL6	431:458	Lactobacillus plantarum VAL6 exposed to stress at pH 3 for 3 h	431:492	The results found that Lactobacillus plantarum VAL6 exposed to stress at pH 3 for 3 h gives the highest exopolysaccharide yield (50.44 g/L) which is 6.4 fold higher than non-stress.
33446763	1	34	theme	8	354:354	arg1	pHs					341:343	pHs	341:343	pHs of 3 and 8	341:354	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	7	35	theme	gene	1310:1313	arg1	expression					1291:1300	the mRNA expression	1282:1300	the mRNA expression of epsF gene	1282:1313	According to our results, the stresses of pH and sodium chloride enhance the production and change the mRNA expression of epsF gene, leading to differences in the monosaccharide composition of exopolysaccharides.
33446763	6	36	theme	epsF	1130:1133	arg1	gene					1135:1138	epsF gene	1130:1138	epsF gene	1130:1138	In addition, stress of pH 8 triggered the mRNA expression of epsF gene resulting in galactose-rich EPS synthesis.
33446763	7	37	theme	pH	1225:1226	arg1	stresses					1213:1220	the stresses	1209:1220	the stresses of pH and sodium chloride	1209:1246	According to our results, the stresses of pH and sodium chloride enhance the production and change the mRNA expression of epsF gene, leading to differences in the monosaccharide composition of exopolysaccharides.
33446763	0	38	theme	VAL6	36:39	arg1	Response					0:7	Response	0:7	Response of Lactobacillus plantarum VAL6 to challenges of pH and sodium chloride stresses.	0:89	Response of Lactobacillus plantarum VAL6 to challenges of pH and sodium chloride stresses.
33446763	2	39	theme	Lactobacillus	431:443	arg1	VAL6					455:458	Lactobacillus plantarum VAL6	431:458	Lactobacillus plantarum VAL6 exposed to stress at pH 3 for 3 h	431:492	The results found that Lactobacillus plantarum VAL6 exposed to stress at pH 3 for 3 h gives the highest exopolysaccharide yield (50.44 g/L) which is 6.4 fold higher than non-stress.
33446763	7	40	theme	epsF	1305:1308	arg1	gene					1310:1313	epsF gene	1305:1313	epsF gene	1305:1313	According to our results, the stresses of pH and sodium chloride enhance the production and change the mRNA expression of epsF gene, leading to differences in the monosaccharide composition of exopolysaccharides.
33446763	3	41	theme	non-stress	728:737	arg1	condition					739:747	non-stress condition	728:747	non-stress condition	728:747	Under pH and sodium chloride stresses, the mannose content in exopolysaccharides decreased while the glucose increased in comparison with non-stress condition.
33446763	3	42	from	content	641:647	arg1	exopolysaccharides					652:669	exopolysaccharides	652:669	exopolysaccharides	652:669	Under pH and sodium chloride stresses, the mannose content in exopolysaccharides decreased while the glucose increased in comparison with non-stress condition.
33446763	4	43	theme	Lactobacillus	864:876	arg1	VAL6					888:891	Lactobacillus plantarum VAL6	864:891	Lactobacillus plantarum VAL6	864:891	The galactose content was highest under stress condition of pH 8 meantime rhamnose content increased sharply when Lactobacillus plantarum VAL6 was stressed at pH 3.
33446763	1	44	dep	pH	337:338	arg1	pHs					341:343	pHs	341:343	pHs of 3 and 8	341:354	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	1	44	dep	pH	337:338	arg1	treatments					396:405	treatments	396:405	treatments	396:405	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	6	45	theme	EPS	1168:1170	arg1	synthesis					1172:1180	galactose-rich EPS synthesis	1153:1180	galactose-rich EPS synthesis	1153:1180	In addition, stress of pH 8 triggered the mRNA expression of epsF gene resulting in galactose-rich EPS synthesis.
33446763	1	46	from	37 °C	207:211	arg1	24 h					188:191	24 h	188:191	24 h of culture at 37 °C with pH 6.8 and without sodium chloride	188:251	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	1	47	theme	different	298:306	arg1	conditions					315:324	different stress conditions	298:324	different stress conditions	298:324	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	1	47	theme	different	298:306	arg1	pH					337:338	pH	337:338	pH (pHs of 3 and 8)	337:355	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	1	47	theme	different	298:306	arg1	concentration					382:394	high sodium chloride concentration	361:394	high sodium chloride concentration	361:394	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	1	48	theme	high	361:364	arg1	concentration					382:394	high sodium chloride concentration	361:394	high sodium chloride concentration	361:394	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	4	49	theme	stress	790:795	arg1	condition					797:805	stress condition	790:805	stress condition of pH 8 meantime rhamnose content increased sharply when Lactobacillus plantarum VAL6 was stressed at pH 3	790:912	The galactose content was highest under stress condition of pH 8 meantime rhamnose content increased sharply when Lactobacillus plantarum VAL6 was stressed at pH 3.
33446763	6	50	theme	galactose-rich	1153:1166	arg1	synthesis					1172:1180	galactose-rich EPS synthesis	1153:1180	galactose-rich EPS synthesis	1153:1180	In addition, stress of pH 8 triggered the mRNA expression of epsF gene resulting in galactose-rich EPS synthesis.
33446763	1	51	theme	stress	308:313	arg1	conditions					315:324	different stress conditions	298:324	different stress conditions	298:324	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	1	51	theme	stress	308:313	arg1	pH					337:338	pH	337:338	pH (pHs of 3 and 8)	337:355	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	1	51	theme	stress	308:313	arg1	concentration					382:394	high sodium chloride concentration	361:394	high sodium chloride concentration	361:394	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	1	52	theme	sodium	366:371	arg1	concentration					382:394	high sodium chloride concentration	361:394	high sodium chloride concentration	361:394	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	4	53	theme	galactose	754:762	arg1	content					764:770	The galactose content	750:770	The galactose content	750:770	The galactose content was highest under stress condition of pH 8 meantime rhamnose content increased sharply when Lactobacillus plantarum VAL6 was stressed at pH 3.
33446763	4	53	theme	galactose	754:762	arg1	highest					776:782	highest	776:782	highest	776:782	The galactose content was highest under stress condition of pH 8 meantime rhamnose content increased sharply when Lactobacillus plantarum VAL6 was stressed at pH 3.
33446763	2	54	theme	exopolysaccharide	512:528	arg1	yield					530:534	the highest exopolysaccharide yield	500:534	the highest exopolysaccharide yield (50.44 g/L) which is 6.4 fold higher than non-stress	500:587	The results found that Lactobacillus plantarum VAL6 exposed to stress at pH 3 for 3 h gives the highest exopolysaccharide yield (50.44 g/L) which is 6.4 fold higher than non-stress.
33446763	2	54	theme	exopolysaccharide	512:528	arg1	higher					566:571	higher	566:571	higher	566:571	The results found that Lactobacillus plantarum VAL6 exposed to stress at pH 3 for 3 h gives the highest exopolysaccharide yield (50.44 g/L) which is 6.4 fold higher than non-stress.
33446763	2	54	theme	exopolysaccharide	512:528	arg1	50.44 g/L					537:545	50.44 g/L	537:545	50.44 g/L	537:545	The results found that Lactobacillus plantarum VAL6 exposed to stress at pH 3 for 3 h gives the highest exopolysaccharide yield (50.44 g/L) which is 6.4 fold higher than non-stress.
33446763	1	55	theme	chloride	373:380	arg1	concentration					382:394	high sodium chloride concentration	361:394	high sodium chloride concentration	361:394	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	7	56	theme	mRNA	1286:1289	arg1	expression					1291:1300	the mRNA expression	1282:1300	the mRNA expression of epsF gene	1282:1313	According to our results, the stresses of pH and sodium chloride enhance the production and change the mRNA expression of epsF gene, leading to differences in the monosaccharide composition of exopolysaccharides.
33446763	1	57	theme	environmental	120:132	arg1	stresses					134:141	environmental stresses	120:141	environmental stresses	120:141	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	7	58	from	differences	1327:1337	arg1	composition					1361:1371	the monosaccharide composition	1342:1371	the monosaccharide composition of exopolysaccharides	1342:1393	According to our results, the stresses of pH and sodium chloride enhance the production and change the mRNA expression of epsF gene, leading to differences in the monosaccharide composition of exopolysaccharides.
33446763	5	59	theme	great	1026:1030	arg1	amounts					1032:1038	great amounts	1026:1038	great amounts	1026:1038	The arabinose content in exopolysaccharides was not detected under non-stress condition but it was recorded in great amounts after 3 h of stress at pH 3.
33446763	3	60	theme	chloride	610:617	arg1	stresses					619:626	pH and sodium chloride stresses	596:626	pH and sodium chloride stresses	596:626	Under pH and sodium chloride stresses, the mannose content in exopolysaccharides decreased while the glucose increased in comparison with non-stress condition.
33446763	6	61	theme	gene	1135:1138	arg1	expression					1116:1125	the mRNA expression	1107:1125	the mRNA expression of epsF gene	1107:1138	In addition, stress of pH 8 triggered the mRNA expression of epsF gene resulting in galactose-rich EPS synthesis.
33446763	7	62	theme	sodium	1232:1237	arg1	chloride					1239:1246	sodium chloride	1232:1246	sodium chloride	1232:1246	According to our results, the stresses of pH and sodium chloride enhance the production and change the mRNA expression of epsF gene, leading to differences in the monosaccharide composition of exopolysaccharides.
33446763	1	63	theme	stresses	134:141	arg1	effect					110:115	the effect	106:115	the effect of environmental stresses on the exopolysaccharide biosynthesis	106:179	To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments.
33446763	0	64	theme	pH	58:59	arg1	challenges					44:53	challenges	44:53	challenges of pH and sodium chloride stresses	44:88	Response of Lactobacillus plantarum VAL6 to challenges of pH and sodium chloride stresses.
33446763	5	65	theme	non-stress	982:991	arg1	condition					993:1001	non-stress condition	982:1001	non-stress condition	982:1001	The arabinose content in exopolysaccharides was not detected under non-stress condition but it was recorded in great amounts after 3 h of stress at pH 3.
34963101	4	0	theme	addition	882:889	arg1	increase					867:874	the increase	863:874	the increase of SA addition	863:889	With the increase of SA addition, the porosity and pore size of BCA gradually reduced and the thickness of pore wall increased.
34963101	2	1	from	ratio	432:436	arg1	microstructure					367:380	the microstructure	363:380	the microstructure	363:380	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	6	2	theme	BCA	1276:1278	arg1	SA					1270:1271	the adding SA	1259:1271	the adding SA of BCA	1259:1278	The mechanical strength of BCA was also significantly enhanced, and the mineralization ability of bioglass was effectively deployed with the adding SA of BCA.
34963101	2	3	theme	infrared	622:629	arg1	spectrometer					642:653	infrared absorption spectrometer	622:653	infrared absorption spectrometer	622:653	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	3	theme	infrared	622:629	arg1	microscopy					591:600	the scanning electron microscopy	569:600	the scanning electron microscopy	569:600	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	7	4	theme	network	1348:1354	arg1	structure					1356:1364	formed 3D network structure	1338:1364	formed 3D network structure	1338:1364	The improved performance of BCA may be attributed to the formed 3D network structure, activated bioavailability and crosslinking ability between chitosan and SA.
34963101	2	5	theme	amount	329:334	arg1	influence					303:311	The influence	299:311	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds	299:513	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	3	6	theme	network	758:764	arg1	structure					766:774	the three-dimensional interconnected network structure	721:774	the three-dimensional interconnected network structure with the high porosity of 82%-87%	721:808	The results show that the BCA composite scaffolds have the three-dimensional interconnected network structure with the high porosity of 82%-87% and a suitable average pore size of 140-200 μm.
34963101	7	7	dep	structure	1356:1364	arg1	the					1334:1336	the	1334:1336	the	1334:1336	The improved performance of BCA may be attributed to the formed 3D network structure, activated bioavailability and crosslinking ability between chitosan and SA.
34963101	7	8	theme	crosslinking	1397:1408	arg1	ability					1410:1416	crosslinking ability	1397:1416	crosslinking ability	1397:1416	The improved performance of BCA may be attributed to the formed 3D network structure, activated bioavailability and crosslinking ability between chitosan and SA.
34963101	2	9	theme	X-ray	603:607	arg1	microscopy					591:600	the scanning electron microscopy	569:600	the scanning electron microscopy	569:600	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	9	theme	X-ray	603:607	arg1	diffraction					609:619	X-ray diffraction	603:619	X-ray diffraction	603:619	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	4	10	theme	SA	879:880	arg1	addition					882:889	SA addition	879:889	SA addition	879:889	With the increase of SA addition, the porosity and pore size of BCA gradually reduced and the thickness of pore wall increased.
34963101	4	11	theme	BCA	922:924	arg1	porosity					896:903	porosity	896:903	porosity	896:903	With the increase of SA addition, the porosity and pore size of BCA gradually reduced and the thickness of pore wall increased.
34963101	4	11	theme	BCA	922:924	arg1	size					914:917	pore size	909:917	pore size	909:917	With the increase of SA addition, the porosity and pore size of BCA gradually reduced and the thickness of pore wall increased.
34963101	3	12	theme	high	785:788	arg1	porosity					790:797	the high porosity	781:797	the high porosity of 82%-87%	781:808	The results show that the BCA composite scaffolds have the three-dimensional interconnected network structure with the high porosity of 82%-87% and a suitable average pore size of 140-200 μm.
34963101	2	13	theme	sodium	339:344	arg1	alginate					346:353	sodium alginate	339:353	sodium alginate (SA)	339:358	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	13	theme	sodium	339:344	arg1	SA					356:357	SA	356:357	SA	356:357	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	3	14	theme	%	808:808	arg1	porosity					790:797	the high porosity	781:797	the high porosity of 82%-87%	781:808	The results show that the BCA composite scaffolds have the three-dimensional interconnected network structure with the high porosity of 82%-87% and a suitable average pore size of 140-200 μm.
34963101	3	15	with	size	838:841	arg1	porosity					790:797	the high porosity	781:797	the high porosity of 82%-87%	781:808	The results show that the BCA composite scaffolds have the three-dimensional interconnected network structure with the high porosity of 82%-87% and a suitable average pore size of 140-200 μm.
34963101	6	16	theme	bioglass	1220:1227	arg1	ability					1209:1215	the mineralization ability	1190:1215	the mineralization ability of bioglass	1190:1227	The mechanical strength of BCA was also significantly enhanced, and the mineralization ability of bioglass was effectively deployed with the adding SA of BCA.
34963101	1	17	theme	Bioglass/chitosan-alginate	139:164	arg1	scaffolds					182:190	Bioglass/chitosan-alginate (BCA) composite scaffolds	139:190	Bioglass/chitosan-alginate (BCA) composite scaffolds with remarkable performance for bone tissue engineering	139:246	Bioglass/chitosan-alginate (BCA) composite scaffolds with remarkable performance for bone tissue engineering are successfully prepared by freeze-drying method.
34963101	8	18	theme	BCA	1461:1463	arg1	scaffolds					1475:1483	BCA composite scaffolds	1461:1483	BCA composite scaffolds	1461:1483	It indicates that BCA composite scaffolds have potential applications in bone issues engineering.
34963101	7	19	theme	formed	1338:1343	arg1	structure					1356:1364	formed 3D network structure	1338:1364	formed 3D network structure	1338:1364	The improved performance of BCA may be attributed to the formed 3D network structure, activated bioavailability and crosslinking ability between chitosan and SA.
34963101	3	20	theme	average	825:831	arg1	size					838:841	a suitable average pore size	814:841	a suitable average pore size of 140-200 μm	814:855	The results show that the BCA composite scaffolds have the three-dimensional interconnected network structure with the high porosity of 82%-87% and a suitable average pore size of 140-200 μm.
34963101	4	21	theme	wall	970:973	arg1	thickness					952:960	the thickness	948:960	the thickness of pore wall	948:973	With the increase of SA addition, the porosity and pore size of BCA gradually reduced and the thickness of pore wall increased.
34963101	1	22	theme	composite	172:180	arg1	scaffolds					182:190	Bioglass/chitosan-alginate (BCA) composite scaffolds	139:190	Bioglass/chitosan-alginate (BCA) composite scaffolds with remarkable performance for bone tissue engineering	139:246	Bioglass/chitosan-alginate (BCA) composite scaffolds with remarkable performance for bone tissue engineering are successfully prepared by freeze-drying method.
34963101	8	23	theme	potential	1490:1498	arg1	applications					1500:1511	potential applications	1490:1511	potential applications	1490:1511	It indicates that BCA composite scaffolds have potential applications in bone issues engineering.
34963101	2	24	theme	microscopy	591:600	arg1	techniques					555:564	various techniques	547:564	various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on	547:663	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	3	25	theme	140-200 μm	846:855	arg1	structure					766:774	the three-dimensional interconnected network structure	721:774	the three-dimensional interconnected network structure with the high porosity of 82%-87%	721:808	The results show that the BCA composite scaffolds have the three-dimensional interconnected network structure with the high porosity of 82%-87% and a suitable average pore size of 140-200 μm.
34963101	3	25	theme	140-200 μm	846:855	arg1	size					838:841	a suitable average pore size	814:841	a suitable average pore size of 140-200 μm	814:855	The results show that the BCA composite scaffolds have the three-dimensional interconnected network structure with the high porosity of 82%-87% and a suitable average pore size of 140-200 μm.
34963101	0	26	theme	mechanical	92:101	arg1	properties					103:112	mechanical properties	92:112	mechanical properties	92:112	Preparation of the bioglass/chitosan-alginate composite scaffolds with high bioactivity and mechanical properties as bone graft materials.
34963101	7	27	theme	3D	1345:1346	arg1	structure					1356:1364	formed 3D network structure	1338:1364	formed 3D network structure	1338:1364	The improved performance of BCA may be attributed to the formed 3D network structure, activated bioavailability and crosslinking ability between chitosan and SA.
34963101	5	28	from	prolongation	1085:1096	arg1	PBS					1117:1119	PBS	1117:1119	PBS	1117:1119	The swelling and degradation ratios decreased gradually with the raising SA and increased with the prolongation of soaking time in PBS.
34963101	2	29	from	ability	480:486	arg1	microstructure					367:380	the microstructure	363:380	the microstructure	363:380	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	30	theme	ratio	413:417	arg1	influence					303:311	The influence	299:311	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds	299:513	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	0	31	theme	graft	122:126	arg1	materials					128:136	bone graft materials	117:136	bone graft materials	117:136	Preparation of the bioglass/chitosan-alginate composite scaffolds with high bioactivity and mechanical properties as bone graft materials.
34963101	5	32	theme	time	1109:1112	arg1	prolongation					1085:1096	the prolongation	1081:1096	the prolongation of soaking time in PBS	1081:1119	The swelling and degradation ratios decreased gradually with the raising SA and increased with the prolongation of soaking time in PBS.
34963101	2	33	theme	mineralization	465:478	arg1	ability					480:486	mineralization ability	465:486	mineralization ability	465:486	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	34	theme	properties	450:459	arg1	influence					303:311	The influence	299:311	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds	299:513	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	35	theme	composite	495:503	arg1	scaffolds					505:513	BCA composite scaffolds	491:513	BCA composite scaffolds	491:513	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	36	theme	degradation	420:430	arg1	ratio					432:436	degradation ratio	420:436	degradation ratio	420:436	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	0	37	theme	composite	46:54	arg1	Preparation					0:10	Preparation	0:10	Preparation of the bioglass/chitosan-alginate composite	0:54	Preparation of the bioglass/chitosan-alginate composite scaffolds with high bioactivity and mechanical properties as bone graft materials.
34963101	7	38	theme	improved	1285:1292	arg1	performance					1294:1304	The improved performance	1281:1304	The improved performance of BCA	1281:1311	The improved performance of BCA may be attributed to the formed 3D network structure, activated bioavailability and crosslinking ability between chitosan and SA.
34963101	2	39	from	ratio	413:417	arg1	microstructure					367:380	the microstructure	363:380	the microstructure	363:380	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	3	40	with	structure	766:774	arg1	porosity					790:797	the high porosity	781:797	the high porosity of 82%-87%	781:808	The results show that the BCA composite scaffolds have the three-dimensional interconnected network structure with the high porosity of 82%-87% and a suitable average pore size of 140-200 μm.
34963101	5	41	theme	swelling	990:997	arg1	ratios					1015:1020	The swelling and degradation ratios	986:1020	The swelling and degradation ratios	986:1020	The swelling and degradation ratios decreased gradually with the raising SA and increased with the prolongation of soaking time in PBS.
34963101	3	42	theme	BCA	692:694	arg1	scaffolds					706:714	the BCA composite scaffolds	688:714	the BCA composite scaffolds	688:714	The results show that the BCA composite scaffolds have the three-dimensional interconnected network structure with the high porosity of 82%-87% and a suitable average pore size of 140-200 μm.
34963101	2	43	theme	pore	393:396	arg1	size					398:401	pore size	393:401	pore size	393:401	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	1	44	theme	freeze-drying	277:289	arg1	method					291:296	freeze-drying method	277:296	freeze-drying method	277:296	Bioglass/chitosan-alginate (BCA) composite scaffolds with remarkable performance for bone tissue engineering are successfully prepared by freeze-drying method.
34963101	0	45	theme	high	71:74	arg1	bioactivity					76:86	high bioactivity	71:86	high bioactivity	71:86	Preparation of the bioglass/chitosan-alginate composite scaffolds with high bioactivity and mechanical properties as bone graft materials.
34963101	5	46	theme	degradation	1003:1013	arg1	ratios					1015:1020	The swelling and degradation ratios	986:1020	The swelling and degradation ratios	986:1020	The swelling and degradation ratios decreased gradually with the raising SA and increased with the prolongation of soaking time in PBS.
34963101	3	47	theme	interconnected	743:756	arg1	structure					766:774	the three-dimensional interconnected network structure	721:774	the three-dimensional interconnected network structure with the high porosity of 82%-87%	721:808	The results show that the BCA composite scaffolds have the three-dimensional interconnected network structure with the high porosity of 82%-87% and a suitable average pore size of 140-200 μm.
34963101	2	48	theme	porosity	383:390	arg1	influence					303:311	The influence	299:311	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds	299:513	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	49	theme	absorption	631:640	arg1	spectrometer					642:653	infrared absorption spectrometer	622:653	infrared absorption spectrometer	622:653	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	49	theme	absorption	631:640	arg1	microscopy					591:600	the scanning electron microscopy	569:600	the scanning electron microscopy	569:600	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	50	from	microstructure	367:380	arg1	influence					303:311	The influence	299:311	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds	299:513	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	6	51	theme	adding	1263:1268	arg1	SA					1270:1271	the adding SA	1259:1271	the adding SA of BCA	1259:1278	The mechanical strength of BCA was also significantly enhanced, and the mineralization ability of bioglass was effectively deployed with the adding SA of BCA.
34963101	2	52	theme	addition	320:327	arg1	amount					329:334	the addition amount	316:334	the addition amount of sodium alginate (SA) on the microstructure	316:380	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	52	theme	addition	320:327	arg1	alginate					346:353	sodium alginate	339:353	sodium alginate (SA)	339:358	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	52	theme	addition	320:327	arg1	SA					356:357	SA	356:357	SA	356:357	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	1	53	theme	remarkable	197:206	arg1	performance					208:218	remarkable performance	197:218	remarkable performance for bone tissue engineering	197:246	Bioglass/chitosan-alginate (BCA) composite scaffolds with remarkable performance for bone tissue engineering are successfully prepared by freeze-drying method.
34963101	2	54	theme	on	662:663	arg1	techniques					555:564	various techniques	547:564	various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on	547:663	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	55	from	porosity	383:390	arg1	microstructure					367:380	the microstructure	363:380	the microstructure	363:380	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	4	56	dep	porosity	896:903	arg1	the					892:894	the	892:894	the	892:894	With the increase of SA addition, the porosity and pore size of BCA gradually reduced and the thickness of pore wall increased.
34963101	2	57	dep	and	655:657	arg1	so					659:660	so	659:660	so	659:660	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	6	58	theme	BCA	1149:1151	arg1	strength					1137:1144	The mechanical strength	1122:1144	The mechanical strength of BCA	1122:1151	The mechanical strength of BCA was also significantly enhanced, and the mineralization ability of bioglass was effectively deployed with the adding SA of BCA.
34963101	3	59	theme	82	802:803	arg1	%					804:804	%	804:804	%	804:804	The results show that the BCA composite scaffolds have the three-dimensional interconnected network structure with the high porosity of 82%-87% and a suitable average pore size of 140-200 μm.
34963101	2	60	theme	alginate	346:353	arg1	alginate					346:353	sodium alginate	339:353	sodium alginate (SA)	339:358	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	60	theme	alginate	346:353	arg1	ratio					413:417	swelling ratio	404:417	swelling ratio	404:417	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	60	theme	alginate	346:353	arg1	properties					450:459	mechanical properties	439:459	mechanical properties	439:459	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	60	theme	alginate	346:353	arg1	SA					356:357	SA	356:357	SA	356:357	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	60	theme	alginate	346:353	arg1	porosity					383:390	porosity	383:390	porosity	383:390	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	60	theme	alginate	346:353	arg1	amount					329:334	the addition amount	316:334	the addition amount of sodium alginate (SA) on the microstructure	316:380	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	60	theme	alginate	346:353	arg1	ratio					432:436	degradation ratio	420:436	degradation ratio	420:436	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	60	theme	alginate	346:353	arg1	ability					480:486	mineralization ability	465:486	mineralization ability	465:486	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	60	theme	alginate	346:353	arg1	size					398:401	pore size	393:401	pore size	393:401	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	1	61	theme	tissue	229:234	arg1	engineering					236:246	bone tissue engineering	224:246	bone tissue engineering	224:246	Bioglass/chitosan-alginate (BCA) composite scaffolds with remarkable performance for bone tissue engineering are successfully prepared by freeze-drying method.
34963101	2	62	theme	scanning	573:580	arg1	spectrometer					642:653	infrared absorption spectrometer	622:653	infrared absorption spectrometer	622:653	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	62	theme	scanning	573:580	arg1	microscopy					591:600	the scanning electron microscopy	569:600	the scanning electron microscopy	569:600	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	62	theme	scanning	573:580	arg1	diffraction					609:619	X-ray diffraction	603:619	X-ray diffraction	603:619	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	1	63	theme	bone	224:227	arg1	engineering					236:246	bone tissue engineering	224:246	bone tissue engineering	224:246	Bioglass/chitosan-alginate (BCA) composite scaffolds with remarkable performance for bone tissue engineering are successfully prepared by freeze-drying method.
34963101	7	64	theme	activated	1367:1375	arg1	bioavailability					1377:1391	activated bioavailability	1367:1391	activated bioavailability	1367:1391	The improved performance of BCA may be attributed to the formed 3D network structure, activated bioavailability and crosslinking ability between chitosan and SA.
34963101	2	65	theme	electron	582:589	arg1	spectrometer					642:653	infrared absorption spectrometer	622:653	infrared absorption spectrometer	622:653	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	65	theme	electron	582:589	arg1	microscopy					591:600	the scanning electron microscopy	569:600	the scanning electron microscopy	569:600	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	65	theme	electron	582:589	arg1	diffraction					609:619	X-ray diffraction	603:619	X-ray diffraction	603:619	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	8	66	theme	composite	1465:1473	arg1	scaffolds					1475:1483	BCA composite scaffolds	1461:1483	BCA composite scaffolds	1461:1483	It indicates that BCA composite scaffolds have potential applications in bone issues engineering.
34963101	3	67	theme	-87	805:807	arg1	%					804:804	%	804:804	%	804:804	The results show that the BCA composite scaffolds have the three-dimensional interconnected network structure with the high porosity of 82%-87% and a suitable average pore size of 140-200 μm.
34963101	5	68	theme	raising	1051:1057	arg1	SA					1059:1060	the raising SA	1047:1060	the raising SA	1047:1060	The swelling and degradation ratios decreased gradually with the raising SA and increased with the prolongation of soaking time in PBS.
34963101	4	69	theme	pore	909:912	arg1	size					914:917	pore size	909:917	pore size	909:917	With the increase of SA addition, the porosity and pore size of BCA gradually reduced and the thickness of pore wall increased.
34963101	6	70	theme	mineralization	1194:1207	arg1	ability					1209:1215	the mineralization ability	1190:1215	the mineralization ability of bioglass	1190:1227	The mechanical strength of BCA was also significantly enhanced, and the mineralization ability of bioglass was effectively deployed with the adding SA of BCA.
34963101	3	71	theme	pore	833:836	arg1	size					838:841	a suitable average pore size	814:841	a suitable average pore size of 140-200 μm	814:855	The results show that the BCA composite scaffolds have the three-dimensional interconnected network structure with the high porosity of 82%-87% and a suitable average pore size of 140-200 μm.
34963101	2	72	from	amount	329:334	arg1	microstructure					367:380	the microstructure	363:380	the microstructure	363:380	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	4	73	theme	pore	965:968	arg1	wall					970:973	pore wall	965:973	pore wall	965:973	With the increase of SA addition, the porosity and pore size of BCA gradually reduced and the thickness of pore wall increased.
34963101	2	74	from	properties	450:459	arg1	microstructure					367:380	the microstructure	363:380	the microstructure	363:380	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	3	75	contain	have	716:719	arg2	structure					766:774	the three-dimensional interconnected network structure	721:774	the three-dimensional interconnected network structure with the high porosity of 82%-87%	721:808	The results show that the BCA composite scaffolds have the three-dimensional interconnected network structure with the high porosity of 82%-87% and a suitable average pore size of 140-200 μm.
34963101	3	75	contain	have	716:719	arg2	size					838:841	a suitable average pore size	814:841	a suitable average pore size of 140-200 μm	814:855	The results show that the BCA composite scaffolds have the three-dimensional interconnected network structure with the high porosity of 82%-87% and a suitable average pore size of 140-200 μm.
34963101	3	75	contain	have	716:719	arg1	scaffolds					706:714	the BCA composite scaffolds	688:714	the BCA composite scaffolds	688:714	The results show that the BCA composite scaffolds have the three-dimensional interconnected network structure with the high porosity of 82%-87% and a suitable average pore size of 140-200 μm.
34963101	3	76	theme	suitable	816:823	arg1	size					838:841	a suitable average pore size	814:841	a suitable average pore size of 140-200 μm	814:855	The results show that the BCA composite scaffolds have the three-dimensional interconnected network structure with the high porosity of 82%-87% and a suitable average pore size of 140-200 μm.
34963101	1	77	with	scaffolds	182:190	arg1	performance					208:218	remarkable performance	197:218	remarkable performance for bone tissue engineering	197:246	Bioglass/chitosan-alginate (BCA) composite scaffolds with remarkable performance for bone tissue engineering are successfully prepared by freeze-drying method.
34963101	2	78	theme	scaffolds	505:513	arg1	influence					303:311	The influence	299:311	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds	299:513	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	8	79	theme	bone	1516:1519	arg1	engineering					1528:1538	bone issues engineering	1516:1538	bone issues engineering	1516:1538	It indicates that BCA composite scaffolds have potential applications in bone issues engineering.
34963101	0	80	theme	bone	117:120	arg1	materials					128:136	bone graft materials	117:136	bone graft materials	117:136	Preparation of the bioglass/chitosan-alginate composite scaffolds with high bioactivity and mechanical properties as bone graft materials.
34963101	2	81	theme	various	547:553	arg1	techniques					555:564	various techniques	547:564	various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on	547:663	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	5	82	theme	soaking	1101:1107	arg1	time					1109:1112	soaking time	1101:1112	soaking time in PBS	1101:1119	The swelling and degradation ratios decreased gradually with the raising SA and increased with the prolongation of soaking time in PBS.
34963101	2	83	theme	swelling	404:411	arg1	ratio					413:417	swelling ratio	404:417	swelling ratio	404:417	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	5	84	from	time	1109:1112	arg1	PBS					1117:1119	PBS	1117:1119	PBS	1117:1119	The swelling and degradation ratios decreased gradually with the raising SA and increased with the prolongation of soaking time in PBS.
34963101	8	85	theme	issues	1521:1526	arg1	engineering					1528:1538	bone issues engineering	1516:1538	bone issues engineering	1516:1538	It indicates that BCA composite scaffolds have potential applications in bone issues engineering.
34963101	7	86	theme	BCA	1309:1311	arg1	performance					1294:1304	The improved performance	1281:1304	The improved performance of BCA	1281:1311	The improved performance of BCA may be attributed to the formed 3D network structure, activated bioavailability and crosslinking ability between chitosan and SA.
34963101	2	87	theme	size	398:401	arg1	influence					303:311	The influence	299:311	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds	299:513	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	0	88	theme	bioglass/chitosan-alginate	19:44	arg1	composite					46:54	the bioglass/chitosan-alginate composite	15:54	the bioglass/chitosan-alginate composite	15:54	Preparation of the bioglass/chitosan-alginate composite scaffolds with high bioactivity and mechanical properties as bone graft materials.
34963101	2	89	theme	mechanical	439:448	arg1	properties					450:459	mechanical properties	439:459	mechanical properties	439:459	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	90	theme	ratio	432:436	arg1	influence					303:311	The influence	299:311	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds	299:513	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	91	from	influence	303:311	arg1	microstructure					367:380	the microstructure	363:380	the microstructure	363:380	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	2	92	theme	BCA	491:493	arg1	scaffolds					505:513	BCA composite scaffolds	491:513	BCA composite scaffolds	491:513	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	5	93	from	PBS	1117:1119	arg1	prolongation					1085:1096	the prolongation	1081:1096	the prolongation of soaking time in PBS	1081:1119	The swelling and degradation ratios decreased gradually with the raising SA and increased with the prolongation of soaking time in PBS.
34963101	2	94	from	size	398:401	arg1	microstructure					367:380	the microstructure	363:380	the microstructure	363:380	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	6	95	theme	mechanical	1126:1135	arg1	strength					1137:1144	The mechanical strength	1122:1144	The mechanical strength of BCA	1122:1151	The mechanical strength of BCA was also significantly enhanced, and the mineralization ability of bioglass was effectively deployed with the adding SA of BCA.
34963101	2	96	theme	ability	480:486	arg1	influence					303:311	The influence	299:311	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds	299:513	The influence of the addition amount of sodium alginate (SA) on the microstructure, porosity, pore size, swelling ratio, degradation ratio, mechanical properties and mineralization ability of BCA composite scaffolds is studied and characterized by various techniques of the scanning electron microscopy, X-ray diffraction, infrared absorption spectrometer and so on.
34963101	3	97	theme	composite	696:704	arg1	scaffolds					706:714	the BCA composite scaffolds	688:714	the BCA composite scaffolds	688:714	The results show that the BCA composite scaffolds have the three-dimensional interconnected network structure with the high porosity of 82%-87% and a suitable average pore size of 140-200 μm.
34963101	8	98	contain	have	1485:1488	arg1	scaffolds					1475:1483	BCA composite scaffolds	1461:1483	BCA composite scaffolds	1461:1483	It indicates that BCA composite scaffolds have potential applications in bone issues engineering.
34963101	8	98	contain	have	1485:1488	arg2	applications					1500:1511	potential applications	1490:1511	potential applications	1490:1511	It indicates that BCA composite scaffolds have potential applications in bone issues engineering.
34963101	3	99	theme	three-dimensional	725:741	arg1	structure					766:774	the three-dimensional interconnected network structure	721:774	the three-dimensional interconnected network structure with the high porosity of 82%-87%	721:808	The results show that the BCA composite scaffolds have the three-dimensional interconnected network structure with the high porosity of 82%-87% and a suitable average pore size of 140-200 μm.
33334294	6	0	theme	scaffolds	869:877	arg1	architectures					852:864	controlled porous architectures	834:864	controlled porous architectures of scaffolds for cartilage tissue regeneration	834:911	3D printing plays a crucial role in the development of controlled porous architectures of scaffolds for cartilage tissue regeneration.
33334294	18	1	theme	composite	3247:3255	arg1	scaffolds					3257:3265	The 3D printed chitosan composite scaffolds	3223:3265	The 3D printed chitosan composite scaffolds	3223:3265	The 3D printed chitosan composite scaffolds show supports for the differentiation of chondrocytes.
33334294	12	2	from	parts	2075:2079	arg1	development					2096:2106	first part development	2085:2106	first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels	2085:2299	The present study is divided into three parts: in first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels.
33334294	7	3	theme	crucial	1094:1100	arg1	role					1102:1105	a crucial role	1092:1105	a crucial role	1092:1105	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	12	4	with	hydrogel	2147:2154	arg1	utilization					2165:2175	the utilization	2161:2175	the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels	2161:2299	The present study is divided into three parts: in first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels.
33334294	11	5	theme	characterization	1902:1917	arg1	development					1814:1824	the development	1810:1824	the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels	1810:2019	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	16	6	theme	models	2931:2936	arg1	files					2919:2923	RESULTS Stereolithography (STL) files	2887:2923	RESULTS Stereolithography (STL) files of 3D models for porous chitosan composite	2887:2966	RESULTS Stereolithography (STL) files of 3D models for porous chitosan composite were developed using Computer-Aided Design (CAD) and printed with a hydrogel flow rate within the range of 0.2-0.25 ml/min.
33334294	20	7	theme	patient-specific	3510:3525	arg1	experiment					3542:3551	patient-specific and form above experiment	3510:3551	patient-specific and form above experiment	3510:3551	CONCLUSION This method helps in regeneration of degenerated cartilage for patient-specific and form above experiment we also concluded that 3D printed chitosan scaffold is best suited for the regeneration of chondrocyte cells.
33334294	6	8	theme	architectures	852:864	arg1	development					819:829	the development	815:829	the development of controlled porous architectures of scaffolds for cartilage tissue regeneration	815:911	3D printing plays a crucial role in the development of controlled porous architectures of scaffolds for cartilage tissue regeneration.
33334294	9	9	theme	chitosan	1574:1581	arg1	scaffolds					1592:1600	printing porous composite chitosan hydrogel scaffolds	1548:1600	printing porous composite chitosan hydrogel scaffolds of desired architecture layer by layer	1548:1639	To achieve as proposed, an in-house 3D paste extruder printer was developed, which is capable of printing porous composite chitosan hydrogel scaffolds of desired architecture layer by layer.
33334294	12	10	theme	chitosan-alginate-gelatin	2121:2145	arg1	hydrogel					2147:2154	composite chitosan-alginate-gelatin hydrogel	2111:2154	composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels	2111:2299	The present study is divided into three parts: in first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels.
33334294	6	11	theme	controlled	834:843	arg1	architectures					852:864	controlled porous architectures	834:864	controlled porous architectures of scaffolds for cartilage tissue regeneration	834:911	3D printing plays a crucial role in the development of controlled porous architectures of scaffolds for cartilage tissue regeneration.
33334294	12	12	theme	house	2183:2187	arg1	printer					2234:2240	house customized assembled paste extruder based 3D printer	2183:2240	house customized assembled paste extruder based 3D printer	2183:2240	The present study is divided into three parts: in first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels.
33334294	4	13	from	septum	467:472	arg1	form					481:484	the form	477:484	the form of hyaline cartilage	477:505	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	20	14	dep	CONCLUSION	3436:3445	arg1	helps					3459:3463	helps	3459:3463	helps in regeneration of degenerated cartilage for patient-specific and form above experiment we also concluded that 3D printed chitosan scaffold is best suited for the regeneration of chondrocyte cells	3459:3660	CONCLUSION This method helps in regeneration of degenerated cartilage for patient-specific and form above experiment we also concluded that 3D printed chitosan scaffold is best suited for the regeneration of chondrocyte cells.
33334294	0	15	theme	3D	0:1	arg1	Scaffold					30:37	3D Printed Chitosan Composite Scaffold	0:37	3D Printed Chitosan Composite Scaffold for Chondrocytes Differentiation.	0:71	3D Printed Chitosan Composite Scaffold for Chondrocytes Differentiation.
33334294	16	16	theme	hydrogel	3036:3043	arg1	rate					3050:3053	a hydrogel flow rate	3034:3053	a hydrogel flow rate within the range of 0.2-0.25 ml/min	3034:3089	RESULTS Stereolithography (STL) files of 3D models for porous chitosan composite were developed using Computer-Aided Design (CAD) and printed with a hydrogel flow rate within the range of 0.2-0.25 ml/min.
33334294	1	17	theme	3D	187:188	arg1	printer					190:196	a costeffective in house designed 3D printer	153:196	a costeffective in house designed 3D printer followed by its characterization	153:229	AIMS Our aim is to develop 3D printed chitosan-gelatin-alginate scaffolds using a costeffective in house designed 3D printer followed by its characterization.
33334294	15	18	theme	positive	2856:2863	arg1	results					2865:2871	positive results	2856:2871	positive results for the same	2856:2884	In the last part of the study, the differentiation of chondrocytes cells was observed with human Mesenchymal Stem Cells (hMSC) on 3D printed scaffolds and showed positive results for the same.
33334294	4	19	theme	various	635:641	arg1	joints					643:648	various joints	635:648	various joints of bones	635:657	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	7	20	theme	stereolithography	1038:1054	arg1	combination					1056:1066	stereolithography combination	1038:1066	stereolithography combination with biomaterials	1038:1084	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	7	20	theme	stereolithography	1038:1054	arg1	bioprinting					988:998	3D bioprinting	985:998	3D bioprinting	985:998	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	15	21	theme	printed	2827:2833	arg1	scaffolds					2835:2843	3D printed scaffolds	2824:2843	3D printed scaffolds	2824:2843	In the last part of the study, the differentiation of chondrocytes cells was observed with human Mesenchymal Stem Cells (hMSC) on 3D printed scaffolds and showed positive results for the same.
33334294	11	22	from	METHODS	1773:1779	arg1	process					1798:1804	present paper process	1784:1804	present paper process	1784:1804	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	2	23	theme	application	315:325	arg1	part					298:301	part	298:301	part of scaffold application	298:325	To observe chondrocyte differentiation on 3D printed scaffolds as part of scaffold application.
33334294	13	24	theme	chitosan	2357:2364	arg1	hydrogel					2386:2393	3D printed chitosan composite scaffolds hydrogel	2346:2393	3D printed chitosan composite scaffolds hydrogel	2346:2393	In the second part, the characterization of 3D printed chitosan composite scaffolds hydrogel is performed for evaluating the morphological, mechanical, and physical properties.
33334294	9	25	theme	3D	1487:1488	arg1	printer					1505:1511	an in-house 3D paste extruder printer	1475:1511	an in-house 3D paste extruder printer	1475:1511	To achieve as proposed, an in-house 3D paste extruder printer was developed, which is capable of printing porous composite chitosan hydrogel scaffolds of desired architecture layer by layer.
33334294	11	26	from	hydrogels	2011:2019	arg1	development					1814:1824	the development	1810:1824	the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels	1810:2019	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	8	27	theme	different	1248:1256	arg1	compositions					1258:1269	different compositions	1248:1269	different compositions of chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity and architectures with the application of 3D printing	1248:1406	OBJECTIVE Our objective is to develop different compositions of chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity and architectures with the application of 3D printing and observe the growth of cartilage on it.
33334294	11	28	theme	composite	1855:1863	arg1	hydrogel					1865:1872	chitosan-alginate-gelatin composite hydrogel	1829:1872	chitosan-alginate-gelatin composite hydrogel	1829:1872	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	5	29	theme	repair	674:679	arg1	tissues					689:695	or repair damaged tissues	671:695	tissues	689:695	To replace or repair damaged tissues due to disorders or trauma, thousands of surgical procedures are performed daily.
33334294	3	30	from	tissue	383:388	arg1	humans					393:398	humans	393:398	humans	393:398	BACKGROUND Cartilage is considered to be a significant tissue in humans.
33334294	2	31	theme	chondrocyte	243:253	arg1	differentiation					255:269	chondrocyte differentiation	243:269	chondrocyte differentiation	243:269	To observe chondrocyte differentiation on 3D printed scaffolds as part of scaffold application.
33334294	13	32	theme	scaffolds	2376:2384	arg1	hydrogel					2386:2393	3D printed chitosan composite scaffolds hydrogel	2346:2393	3D printed chitosan composite scaffolds hydrogel	2346:2393	In the second part, the characterization of 3D printed chitosan composite scaffolds hydrogel is performed for evaluating the morphological, mechanical, and physical properties.
33334294	20	33	theme	printed	3579:3585	arg1	scaffold					3596:3603	3D printed chitosan scaffold	3576:3603	3D printed chitosan scaffold	3576:3603	CONCLUSION This method helps in regeneration of degenerated cartilage for patient-specific and form above experiment we also concluded that 3D printed chitosan scaffold is best suited for the regeneration of chondrocyte cells.
33334294	3	34	theme	BACKGROUND	328:337	arg1	tissue					383:388	a significant tissue	369:388	a significant tissue	369:388	BACKGROUND Cartilage is considered to be a significant tissue in humans.
33334294	3	34	theme	BACKGROUND	328:337	arg1	Cartilage					339:347	BACKGROUND Cartilage	328:347	BACKGROUND Cartilage	328:347	BACKGROUND Cartilage is considered to be a significant tissue in humans.
33334294	12	35	theme	chitosan	2272:2279	arg1	hydrogels					2291:2299	printing chitosan composite hydrogels	2263:2299	printing chitosan composite hydrogels	2263:2299	The present study is divided into three parts: in first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels.
33334294	12	36	theme	composite	2111:2119	arg1	hydrogel					2147:2154	composite chitosan-alginate-gelatin hydrogel	2111:2154	composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels	2111:2299	The present study is divided into three parts: in first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels.
33334294	7	37	theme	3D	1016:1017	arg1	bioprinting					988:998	3D bioprinting	985:998	3D bioprinting	985:998	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	7	37	theme	3D	1016:1017	arg1	LAB					1032:1034	LAB	1032:1034	LAB	1032:1034	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	7	37	theme	3D	1016:1017	arg1	Bioprinting					1019:1029	Laser-assisted 3D Bioprinting	1001:1029	Laser-assisted 3D Bioprinting (LAB)	1001:1035	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	11	38	theme	chitosan	1992:1999	arg1	hydrogels					2011:2019	3D printed chitosan composite hydrogels	1981:2019	3D printed chitosan composite hydrogels	1981:2019	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	1	39	theme	chitosan-gelatin-alginate	111:135	arg1	scaffolds					137:145	3D printed chitosan-gelatin-alginate scaffolds	100:145	3D printed chitosan-gelatin-alginate scaffolds	100:145	AIMS Our aim is to develop 3D printed chitosan-gelatin-alginate scaffolds using a costeffective in house designed 3D printer followed by its characterization.
33334294	6	40	theme	cartilage	883:891	arg1	regeneration					900:911	cartilage tissue regeneration	883:911	cartilage tissue regeneration	883:911	3D printing plays a crucial role in the development of controlled porous architectures of scaffolds for cartilage tissue regeneration.
33334294	4	41	theme	rib	430:432	arg1	septum					467:472	the ear, nasal septum	452:472	the ear, nasal septum in the form of hyaline cartilage	452:505	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	4	41	theme	rib	430:432	arg1	cage					434:437	the rib cage	426:437	the rib cage	426:437	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	4	41	theme	rib	430:432	arg1	lobe					444:447	the lobe	440:447	the lobe of the ear, nasal septum in the form of hyaline cartilage	440:505	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	8	42	theme	composite	1300:1308	arg1	scaffolds					1319:1327	chitosan-gelatin-alginate composite hydrogel scaffolds	1274:1327	chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity	1274:1352	OBJECTIVE Our objective is to develop different compositions of chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity and architectures with the application of 3D printing and observe the growth of cartilage on it.
33334294	9	43	theme	composite	1564:1572	arg1	scaffolds					1592:1600	printing porous composite chitosan hydrogel scaffolds	1548:1600	printing porous composite chitosan hydrogel scaffolds of desired architecture layer by layer	1548:1639	To achieve as proposed, an in-house 3D paste extruder printer was developed, which is capable of printing porous composite chitosan hydrogel scaffolds of desired architecture layer by layer.
33334294	17	44	theme	mechanical	3167:3176	arg1	porosity					3149:3156	optimum porosity	3141:3156	optimum porosity	3141:3156	The prepared scaffolds are highly porous, having optimum porosity, optimal mechanical strength to sustain the cartilage formation.
33334294	17	44	theme	mechanical	3167:3176	arg1	strength					3178:3185	optimal mechanical strength	3159:3185	optimal mechanical strength to sustain the cartilage formation	3159:3220	The prepared scaffolds are highly porous, having optimum porosity, optimal mechanical strength to sustain the cartilage formation.
33334294	2	45	theme	printed	277:283	arg1	scaffolds					285:293	3D printed scaffolds	274:293	3D printed scaffolds as part of scaffold application	274:325	To observe chondrocyte differentiation on 3D printed scaffolds as part of scaffold application.
33334294	7	46	theme	inkjet	957:962	arg1	extrusion					972:980	inkjet, micro- extrusion	957:980	inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials	957:1084	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	9	47	theme	extruder	1496:1503	arg1	printer					1505:1511	an in-house 3D paste extruder printer	1475:1511	an in-house 3D paste extruder printer	1475:1511	To achieve as proposed, an in-house 3D paste extruder printer was developed, which is capable of printing porous composite chitosan hydrogel scaffolds of desired architecture layer by layer.
33334294	12	48	theme	hydrogel	2147:2154	arg1	development					2096:2106	first part development	2085:2106	first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels	2085:2299	The present study is divided into three parts: in first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels.
33334294	16	49	theme	3D	2928:2929	arg1	models					2931:2936	3D models	2928:2936	3D models for porous chitosan composite	2928:2966	RESULTS Stereolithography (STL) files of 3D models for porous chitosan composite were developed using Computer-Aided Design (CAD) and printed with a hydrogel flow rate within the range of 0.2-0.25 ml/min.
33334294	8	50	theme	scaffolds	1319:1327	arg1	compositions					1258:1269	different compositions	1248:1269	different compositions of chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity and architectures with the application of 3D printing	1248:1406	OBJECTIVE Our objective is to develop different compositions of chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity and architectures with the application of 3D printing and observe the growth of cartilage on it.
33334294	16	51	theme	Stereolithography	2895:2911	arg1	files					2919:2923	RESULTS Stereolithography (STL) files	2887:2923	RESULTS Stereolithography (STL) files of 3D models for porous chitosan composite	2887:2966	RESULTS Stereolithography (STL) files of 3D models for porous chitosan composite were developed using Computer-Aided Design (CAD) and printed with a hydrogel flow rate within the range of 0.2-0.25 ml/min.
33334294	4	52	from	ribs	519:522	arg1	form					570:573	the form	566:573	the form of fibrocartilage, meniscus, larynx, epiglottis	566:621	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	1	53	theme	costeffective	155:167	arg1	printer					190:196	a costeffective in house designed 3D printer	153:196	a costeffective in house designed 3D printer followed by its characterization	153:229	AIMS Our aim is to develop 3D printed chitosan-gelatin-alginate scaffolds using a costeffective in house designed 3D printer followed by its characterization.
33334294	14	54	theme	swelling	2640:2647	arg1	property					2649:2656	swelling property	2640:2656	swelling property	2640:2656	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	14	54	theme	swelling	2640:2647	arg1	Spectroscopy					2561:2572	Infrared Spectroscopy	2552:2572	Infrared Spectroscopy (FTIR)	2552:2579	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	8	55	theme	controlled	1334:1343	arg1	porosity					1345:1352	controlled porosity	1334:1352	controlled porosity	1334:1352	OBJECTIVE Our objective is to develop different compositions of chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity and architectures with the application of 3D printing and observe the growth of cartilage on it.
33334294	14	56	theme	Infrared	2552:2559	arg1	porosity					2679:2686	porosity	2679:2686	porosity	2679:2686	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	14	56	theme	Infrared	2552:2559	arg1	property					2649:2656	swelling property	2640:2656	swelling property	2640:2656	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	14	56	theme	Infrared	2552:2559	arg1	etc					2689:2691	etc	2689:2691	etc	2689:2691	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	14	56	theme	Infrared	2552:2559	arg1	Diffraction					2588:2598	X-Ray Diffraction	2582:2598	X-Ray Diffraction(XRD)	2582:2603	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	14	56	theme	Infrared	2552:2559	arg1	Spectroscopy					2561:2572	Infrared Spectroscopy	2552:2572	Infrared Spectroscopy (FTIR)	2552:2579	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	14	56	theme	Infrared	2552:2559	arg1	SEM					2635:2637	Scanning Electron Microscopy SEM	2606:2637	Scanning Electron Microscopy SEM	2606:2637	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	14	56	theme	Infrared	2552:2559	arg1	testing					2670:2676	mechanical testing	2659:2676	mechanical testing	2659:2676	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	14	56	theme	Infrared	2552:2559	arg1	FTIR					2575:2578	FTIR	2575:2578	FTIR	2575:2578	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	11	57	theme	present	1784:1790	arg1	paper					1792:1796	present paper	1784:1796	present paper process	1784:1804	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	7	58	theme	short	1190:1194	arg1	frame					1203:1207	a short period frame	1188:1207	a short period frame	1188:1207	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	7	59	theme	3D	929:930	arg1	technology					941:950	3D printing technology	929:950	3D printing technology	929:950	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	11	60	from	hydrogel	1865:1872	arg1	hydrogels					2011:2019	3D printed chitosan composite hydrogels	1981:2019	3D printed chitosan composite hydrogels	1981:2019	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	11	61	from	characterization	1902:1917	arg1	hydrogels					2011:2019	3D printed chitosan composite hydrogels	1981:2019	3D printed chitosan composite hydrogels	1981:2019	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	12	62	theme	hydrogels	2291:2299	arg1	capable					2252:2258	capable	2252:2258	capable	2252:2258	The present study is divided into three parts: in first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels.
33334294	4	63	dep	present	407:413	arg1	septum					467:472	the ear, nasal septum	452:472	the ear, nasal septum in the form of hyaline cartilage	452:505	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	4	63	dep	present	407:413	arg1	joints					643:648	various joints	635:648	various joints of bones	635:657	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	4	63	dep	present	407:413	arg1	cage					434:437	the rib cage	426:437	the rib cage	426:437	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	4	63	dep	present	407:413	arg1	lobe					444:447	the lobe	440:447	the lobe of the ear, nasal septum in the form of hyaline cartilage	440:505	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	19	64	theme	in-vivo	3353:3359	arg1	regeneration					3361:3372	in-vivo regeneration	3353:3372	in-vivo regeneration of cartilage for patients having related cartilage disorders	3353:3433	The above study is helpful for in-vivo regeneration of cartilage for patients having related cartilage disorders.
33334294	20	65	theme	cells	3656:3660	arg1	regeneration					3628:3639	the regeneration	3624:3639	the regeneration of chondrocyte cells	3624:3660	CONCLUSION This method helps in regeneration of degenerated cartilage for patient-specific and form above experiment we also concluded that 3D printed chitosan scaffold is best suited for the regeneration of chondrocyte cells.
33334294	18	66	theme	printed	3230:3236	arg1	scaffolds					3257:3265	The 3D printed chitosan composite scaffolds	3223:3265	The 3D printed chitosan composite scaffolds	3223:3265	The 3D printed chitosan composite scaffolds show supports for the differentiation of chondrocytes.
33334294	1	67	dep	AIMS	73:76	arg1	is					86:87	is	86:87	is to develop 3D printed chitosan-gelatin-alginate scaffolds using a costeffective in house designed 3D printer followed by its characterization	86:229	AIMS Our aim is to develop 3D printed chitosan-gelatin-alginate scaffolds using a costeffective in house designed 3D printer followed by its characterization.
33334294	11	68	theme	3D	1981:1982	arg1	hydrogels					2011:2019	3D printed chitosan composite hydrogels	1981:2019	3D printed chitosan composite hydrogels	1981:2019	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	20	69	theme	cartilage	3496:3504	arg1	regeneration					3468:3479	regeneration	3468:3479	regeneration of degenerated cartilage for patient-specific and form above experiment	3468:3551	CONCLUSION This method helps in regeneration of degenerated cartilage for patient-specific and form above experiment we also concluded that 3D printed chitosan scaffold is best suited for the regeneration of chondrocyte cells.
33334294	4	70	theme	septum	467:472	arg1	septum					467:472	the ear, nasal septum	452:472	the ear, nasal septum in the form of hyaline cartilage	452:505	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	4	70	theme	septum	467:472	arg1	cage					434:437	the rib cage	426:437	the rib cage	426:437	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	4	70	theme	septum	467:472	arg1	lobe					444:447	the lobe	440:447	the lobe of the ear, nasal septum in the form of hyaline cartilage	440:505	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	11	71	theme	observation	1924:1934	arg1	development					1814:1824	the development	1810:1824	the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels	1810:2019	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	7	72	theme	articulating	1152:1163	arg1	cartilage					1165:1173	patient-specific articulating cartilage	1135:1173	patient-specific articulating cartilage	1135:1173	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	14	73	theme	Electron	2615:2622	arg1	Microscopy					2624:2633	Scanning Electron Microscopy	2606:2633	Scanning Electron Microscopy SEM	2606:2637	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	9	74	theme	porous	1557:1562	arg1	scaffolds					1592:1600	printing porous composite chitosan hydrogel scaffolds	1548:1600	printing porous composite chitosan hydrogel scaffolds of desired architecture layer by layer	1548:1639	To achieve as proposed, an in-house 3D paste extruder printer was developed, which is capable of printing porous composite chitosan hydrogel scaffolds of desired architecture layer by layer.
33334294	19	75	theme	related	3407:3413	arg1	disorders					3425:3433	related cartilage disorders	3407:3433	related cartilage disorders	3407:3433	The above study is helpful for in-vivo regeneration of cartilage for patients having related cartilage disorders.
33334294	8	76	theme	3D	1396:1397	arg1	printing					1399:1406	3D printing	1396:1406	3D printing	1396:1406	OBJECTIVE Our objective is to develop different compositions of chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity and architectures with the application of 3D printing and observe the growth of cartilage on it.
33334294	13	77	theme	mechanical	2442:2451	arg1	properties					2467:2476	the morphological, mechanical, and physical properties	2423:2476	the morphological, mechanical, and physical properties	2423:2476	In the second part, the characterization of 3D printed chitosan composite scaffolds hydrogel is performed for evaluating the morphological, mechanical, and physical properties.
33334294	10	78	theme	chitosan	1683:1690	arg1	scaffolds					1702:1710	3D printed chitosan composite scaffolds	1672:1710	3D printed chitosan composite scaffolds	1672:1710	After the characterization of 3D printed chitosan composite scaffolds, the differentiation of chondrocyte was observed using hMSC.
33334294	0	79	theme	Chitosan	11:18	arg1	Scaffold					30:37	3D Printed Chitosan Composite Scaffold	0:37	3D Printed Chitosan Composite Scaffold for Chondrocytes Differentiation.	0:71	3D Printed Chitosan Composite Scaffold for Chondrocytes Differentiation.
33334294	14	80	theme	Scanning	2606:2613	arg1	Microscopy					2624:2633	Scanning Electron Microscopy	2606:2633	Scanning Electron Microscopy SEM	2606:2637	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	1	81	theme	3D	100:101	arg1	scaffolds					137:145	3D printed chitosan-gelatin-alginate scaffolds	100:145	3D printed chitosan-gelatin-alginate scaffolds	100:145	AIMS Our aim is to develop 3D printed chitosan-gelatin-alginate scaffolds using a costeffective in house designed 3D printer followed by its characterization.
33334294	7	82	from	Advancement	914:924	arg1	technology					941:950	3D printing technology	929:950	3D printing technology	929:950	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	11	83	theme	chondrocytes	1959:1970	arg1	cells					1972:1976	chondrocytes cells	1959:1976	chondrocytes cells	1959:1976	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	10	84	theme	3D	1672:1673	arg1	scaffolds					1702:1710	3D printed chitosan composite scaffolds	1672:1710	3D printed chitosan composite scaffolds	1672:1710	After the characterization of 3D printed chitosan composite scaffolds, the differentiation of chondrocyte was observed using hMSC.
33334294	7	85	theme	quick	1114:1118	arg1	development					1120:1130	the quick development	1110:1130	the quick development of patient-specific articulating cartilage	1110:1173	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	9	86	theme	scaffolds	1592:1600	arg1	capable					1537:1543	capable	1537:1543	capable	1537:1543	To achieve as proposed, an in-house 3D paste extruder printer was developed, which is capable of printing porous composite chitosan hydrogel scaffolds of desired architecture layer by layer.
33334294	4	87	theme	cartilage	497:505	arg1	form					481:484	the form	477:484	the form of hyaline cartilage	477:505	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	15	88	theme	chondrocytes	2748:2759	arg1	cells					2761:2765	chondrocytes cells	2748:2765	chondrocytes cells	2748:2765	In the last part of the study, the differentiation of chondrocytes cells was observed with human Mesenchymal Stem Cells (hMSC) on 3D printed scaffolds and showed positive results for the same.
33334294	20	89	dep	helps	3459:3463	arg1	concluded					3561:3569	concluded	3561:3569	concluded that 3D printed chitosan scaffold is best suited for the regeneration of chondrocyte cells	3561:3660	CONCLUSION This method helps in regeneration of degenerated cartilage for patient-specific and form above experiment we also concluded that 3D printed chitosan scaffold is best suited for the regeneration of chondrocyte cells.
33334294	11	90	theme	printing	1878:1885	arg1	development					1814:1824	the development	1810:1824	the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels	1810:2019	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	5	91	theme	procedures	747:756	arg1	thousands					725:733	thousands	725:733	thousands of surgical procedures	725:756	To replace or repair damaged tissues due to disorders or trauma, thousands of surgical procedures are performed daily.
33334294	16	92	theme	chitosan	2949:2956	arg1	composite					2958:2966	porous chitosan composite	2942:2966	porous chitosan composite	2942:2966	RESULTS Stereolithography (STL) files of 3D models for porous chitosan composite were developed using Computer-Aided Design (CAD) and printed with a hydrogel flow rate within the range of 0.2-0.25 ml/min.
33334294	9	93	theme	desired	1605:1611	arg1	layer					1626:1630	desired architecture layer	1605:1630	desired architecture layer by layer	1605:1639	To achieve as proposed, an in-house 3D paste extruder printer was developed, which is capable of printing porous composite chitosan hydrogel scaffolds of desired architecture layer by layer.
33334294	12	94	theme	extruder	2216:2223	arg1	printer					2234:2240	house customized assembled paste extruder based 3D printer	2183:2240	house customized assembled paste extruder based 3D printer	2183:2240	The present study is divided into three parts: in first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels.
33334294	14	95	theme	composite	2492:2500	arg1	scaffolds					2502:2510	The prepared composite scaffolds	2479:2510	The prepared composite scaffolds	2479:2510	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	10	96	theme	scaffolds	1702:1710	arg1	characterization					1652:1667	the characterization	1648:1667	the characterization of 3D printed chitosan composite scaffolds	1648:1710	After the characterization of 3D printed chitosan composite scaffolds, the differentiation of chondrocyte was observed using hMSC.
33334294	14	97	theme	X-Ray	2582:2586	arg1	Diffraction					2588:2598	X-Ray Diffraction	2582:2598	X-Ray Diffraction(XRD)	2582:2603	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	14	97	theme	X-Ray	2582:2586	arg1	Spectroscopy					2561:2572	Infrared Spectroscopy	2552:2572	Infrared Spectroscopy (FTIR)	2552:2579	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	14	97	theme	X-Ray	2582:2586	arg1	XRD					2600:2602	XRD	2600:2602	XRD	2600:2602	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	9	98	theme	layer	1626:1630	arg1	scaffolds					1592:1600	printing porous composite chitosan hydrogel scaffolds	1548:1600	printing porous composite chitosan hydrogel scaffolds of desired architecture layer by layer	1548:1639	To achieve as proposed, an in-house 3D paste extruder printer was developed, which is capable of printing porous composite chitosan hydrogel scaffolds of desired architecture layer by layer.
33334294	13	99	theme	second	2309:2314	arg1	part					2316:2319	the second part	2305:2319	the second part	2305:2319	In the second part, the characterization of 3D printed chitosan composite scaffolds hydrogel is performed for evaluating the morphological, mechanical, and physical properties.
33334294	17	100	theme	optimal	3159:3165	arg1	porosity					3149:3156	optimum porosity	3141:3156	optimum porosity	3141:3156	The prepared scaffolds are highly porous, having optimum porosity, optimal mechanical strength to sustain the cartilage formation.
33334294	17	100	theme	optimal	3159:3165	arg1	strength					3178:3185	optimal mechanical strength	3159:3185	optimal mechanical strength to sustain the cartilage formation	3159:3220	The prepared scaffolds are highly porous, having optimum porosity, optimal mechanical strength to sustain the cartilage formation.
33334294	12	101	theme	3D	2231:2232	arg1	printer					2234:2240	house customized assembled paste extruder based 3D printer	2183:2240	house customized assembled paste extruder based 3D printer	2183:2240	The present study is divided into three parts: in first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels.
33334294	4	102	dep	cage	434:437	arg1	in					508:509	in	508:509	in	508:509	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	20	103	theme	above	3536:3540	arg1	experiment					3542:3551	patient-specific and form above experiment	3510:3551	patient-specific and form above experiment	3510:3551	CONCLUSION This method helps in regeneration of degenerated cartilage for patient-specific and form above experiment we also concluded that 3D printed chitosan scaffold is best suited for the regeneration of chondrocyte cells.
33334294	16	104	theme	Computer-Aided	2989:3002	arg1	Design					3004:3009	Computer-Aided Design	2989:3009	Computer-Aided Design (CAD)	2989:3015	RESULTS Stereolithography (STL) files of 3D models for porous chitosan composite were developed using Computer-Aided Design (CAD) and printed with a hydrogel flow rate within the range of 0.2-0.25 ml/min.
33334294	16	104	theme	Computer-Aided	2989:3002	arg1	CAD					3012:3014	CAD	3012:3014	CAD	3012:3014	RESULTS Stereolithography (STL) files of 3D models for porous chitosan composite were developed using Computer-Aided Design (CAD) and printed with a hydrogel flow rate within the range of 0.2-0.25 ml/min.
33334294	4	105	theme	fibrocartilage	578:591	arg1	form					570:573	the form	566:573	the form of fibrocartilage, meniscus, larynx, epiglottis	566:621	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	16	106	theme	flow	3045:3048	arg1	rate					3050:3053	a hydrogel flow rate	3034:3053	a hydrogel flow rate within the range of 0.2-0.25 ml/min	3034:3089	RESULTS Stereolithography (STL) files of 3D models for porous chitosan composite were developed using Computer-Aided Design (CAD) and printed with a hydrogel flow rate within the range of 0.2-0.25 ml/min.
33334294	15	107	theme	3D	2824:2825	arg1	scaffolds					2835:2843	3D printed scaffolds	2824:2843	3D printed scaffolds	2824:2843	In the last part of the study, the differentiation of chondrocytes cells was observed with human Mesenchymal Stem Cells (hMSC) on 3D printed scaffolds and showed positive results for the same.
33334294	11	108	theme	morphological	1888:1900	arg1	characterization					1902:1917	morphological characterization	1888:1917	morphological characterization	1888:1917	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	4	109	dep	ear	456:458	arg1	nasal					461:465	nasal	461:465	nasal	461:465	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	9	110	theme	in-house	1478:1485	arg1	printer					1505:1511	an in-house 3D paste extruder printer	1475:1511	an in-house 3D paste extruder printer	1475:1511	To achieve as proposed, an in-house 3D paste extruder printer was developed, which is capable of printing porous composite chitosan hydrogel scaffolds of desired architecture layer by layer.
33334294	4	111	theme	intervertebral	542:555	arg1	discs					557:561	intervertebral discs	542:561	intervertebral discs	542:561	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	4	111	theme	intervertebral	542:555	arg1	ribs					519:522	ribs	519:522	ribs	519:522	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	16	112	theme	porous	2942:2947	arg1	composite					2958:2966	porous chitosan composite	2942:2966	porous chitosan composite	2942:2966	RESULTS Stereolithography (STL) files of 3D models for porous chitosan composite were developed using Computer-Aided Design (CAD) and printed with a hydrogel flow rate within the range of 0.2-0.25 ml/min.
33334294	4	113	from	form	481:484	arg1	septum					467:472	the ear, nasal septum	452:472	the ear, nasal septum in the form of hyaline cartilage	452:505	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	4	113	from	form	481:484	arg1	cage					434:437	the rib cage	426:437	the rib cage	426:437	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	4	113	from	form	481:484	arg1	lobe					444:447	the lobe	440:447	the lobe of the ear, nasal septum in the form of hyaline cartilage	440:505	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	2	114	theme	scaffold	306:313	arg1	application					315:325	scaffold application	306:325	scaffold application	306:325	To observe chondrocyte differentiation on 3D printed scaffolds as part of scaffold application.
33334294	12	115	theme	present	2039:2045	arg1	study					2047:2051	The present study	2035:2051	The present study	2035:2051	The present study is divided into three parts: in first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels.
33334294	6	116	theme	porous	845:850	arg1	architectures					852:864	controlled porous architectures	834:864	controlled porous architectures of scaffolds for cartilage tissue regeneration	834:911	3D printing plays a crucial role in the development of controlled porous architectures of scaffolds for cartilage tissue regeneration.
33334294	11	117	theme	3D	1875:1876	arg1	printing					1878:1885	3D printing	1875:1885	3D printing	1875:1885	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	17	118	theme	prepared	3096:3103	arg1	scaffolds					3105:3113	The prepared scaffolds	3092:3113	The prepared scaffolds	3092:3113	The prepared scaffolds are highly porous, having optimum porosity, optimal mechanical strength to sustain the cartilage formation.
33334294	17	118	theme	prepared	3096:3103	arg1	porous					3126:3131	porous	3126:3131	porous	3126:3131	The prepared scaffolds are highly porous, having optimum porosity, optimal mechanical strength to sustain the cartilage formation.
33334294	12	119	theme	assembled	2200:2208	arg1	printer					2234:2240	house customized assembled paste extruder based 3D printer	2183:2240	house customized assembled paste extruder based 3D printer	2183:2240	The present study is divided into three parts: in first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels.
33334294	1	120	theme	designed	178:185	arg1	printer					190:196	a costeffective in house designed 3D printer	153:196	a costeffective in house designed 3D printer followed by its characterization	153:229	AIMS Our aim is to develop 3D printed chitosan-gelatin-alginate scaffolds using a costeffective in house designed 3D printer followed by its characterization.
33334294	0	121	theme	Chondrocytes	43:54	arg1	Differentiation					56:70	Chondrocytes Differentiation	43:70	Chondrocytes Differentiation	43:70	3D Printed Chitosan Composite Scaffold for Chondrocytes Differentiation.
33334294	13	122	theme	hydrogel	2386:2393	arg1	characterization					2326:2341	the characterization	2322:2341	the characterization of 3D printed chitosan composite scaffolds hydrogel	2322:2393	In the second part, the characterization of 3D printed chitosan composite scaffolds hydrogel is performed for evaluating the morphological, mechanical, and physical properties.
33334294	15	123	theme	Stem	2803:2806	arg1	hMSC					2815:2818	hMSC	2815:2818	hMSC	2815:2818	In the last part of the study, the differentiation of chondrocytes cells was observed with human Mesenchymal Stem Cells (hMSC) on 3D printed scaffolds and showed positive results for the same.
33334294	15	123	theme	Stem	2803:2806	arg1	Cells					2808:2812	human Mesenchymal Stem Cells	2785:2812	human Mesenchymal Stem Cells (hMSC)	2785:2819	In the last part of the study, the differentiation of chondrocytes cells was observed with human Mesenchymal Stem Cells (hMSC) on 3D printed scaffolds and showed positive results for the same.
33334294	12	124	theme	first	2085:2089	arg1	development					2096:2106	first part development	2085:2106	first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels	2085:2299	The present study is divided into three parts: in first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels.
33334294	16	125	theme	ml/min	3084:3089	arg1	range					3066:3070	the range	3062:3070	the range of 0.2-0.25 ml/min	3062:3089	RESULTS Stereolithography (STL) files of 3D models for porous chitosan composite were developed using Computer-Aided Design (CAD) and printed with a hydrogel flow rate within the range of 0.2-0.25 ml/min.
33334294	20	126	theme	chitosan	3587:3594	arg1	scaffold					3596:3603	3D printed chitosan scaffold	3576:3603	3D printed chitosan scaffold	3576:3603	CONCLUSION This method helps in regeneration of degenerated cartilage for patient-specific and form above experiment we also concluded that 3D printed chitosan scaffold is best suited for the regeneration of chondrocyte cells.
33334294	11	127	from	development	1814:1824	arg1	hydrogels					2011:2019	3D printed chitosan composite hydrogels	1981:2019	3D printed chitosan composite hydrogels	1981:2019	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	12	128	theme	customized	2189:2198	arg1	printer					2234:2240	house customized assembled paste extruder based 3D printer	2183:2240	house customized assembled paste extruder based 3D printer	2183:2240	The present study is divided into three parts: in first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels.
33334294	15	129	theme	Mesenchymal	2791:2801	arg1	hMSC					2815:2818	hMSC	2815:2818	hMSC	2815:2818	In the last part of the study, the differentiation of chondrocytes cells was observed with human Mesenchymal Stem Cells (hMSC) on 3D printed scaffolds and showed positive results for the same.
33334294	15	129	theme	Mesenchymal	2791:2801	arg1	Cells					2808:2812	human Mesenchymal Stem Cells	2785:2812	human Mesenchymal Stem Cells (hMSC)	2785:2819	In the last part of the study, the differentiation of chondrocytes cells was observed with human Mesenchymal Stem Cells (hMSC) on 3D printed scaffolds and showed positive results for the same.
33334294	16	130	theme	RESULTS	2887:2893	arg1	Stereolithography					2895:2911	RESULTS Stereolithography	2887:2911	RESULTS Stereolithography (STL) files of 3D models for porous chitosan composite	2887:2966	RESULTS Stereolithography (STL) files of 3D models for porous chitosan composite were developed using Computer-Aided Design (CAD) and printed with a hydrogel flow rate within the range of 0.2-0.25 ml/min.
33334294	16	130	theme	RESULTS	2887:2893	arg1	STL					2914:2916	STL	2914:2916	STL	2914:2916	RESULTS Stereolithography (STL) files of 3D models for porous chitosan composite were developed using Computer-Aided Design (CAD) and printed with a hydrogel flow rate within the range of 0.2-0.25 ml/min.
33334294	13	131	theme	physical	2458:2465	arg1	properties					2467:2476	the morphological, mechanical, and physical properties	2423:2476	the morphological, mechanical, and physical properties	2423:2476	In the second part, the characterization of 3D printed chitosan composite scaffolds hydrogel is performed for evaluating the morphological, mechanical, and physical properties.
33334294	13	132	theme	printed	2349:2355	arg1	hydrogel					2386:2393	3D printed chitosan composite scaffolds hydrogel	2346:2393	3D printed chitosan composite scaffolds hydrogel	2346:2393	In the second part, the characterization of 3D printed chitosan composite scaffolds hydrogel is performed for evaluating the morphological, mechanical, and physical properties.
33334294	12	133	theme	part	2091:2094	arg1	development					2096:2106	first part development	2085:2106	first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels	2085:2299	The present study is divided into three parts: in first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels.
33334294	7	134	from	extrusion	972:980	arg1	combination					1056:1066	stereolithography combination	1038:1066	stereolithography combination with biomaterials	1038:1084	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	7	134	from	extrusion	972:980	arg1	bioprinting					988:998	3D bioprinting	985:998	3D bioprinting	985:998	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	7	134	from	extrusion	972:980	arg1	Bioprinting					1019:1029	Laser-assisted 3D Bioprinting	1001:1029	Laser-assisted 3D Bioprinting (LAB)	1001:1035	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	19	135	theme	cartilage	3377:3385	arg1	regeneration					3361:3372	in-vivo regeneration	3353:3372	in-vivo regeneration of cartilage for patients having related cartilage disorders	3353:3433	The above study is helpful for in-vivo regeneration of cartilage for patients having related cartilage disorders.
33334294	11	136	theme	composite	2001:2009	arg1	hydrogels					2011:2019	3D printed chitosan composite hydrogels	1981:2019	3D printed chitosan composite hydrogels	1981:2019	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	8	137	from	growth	1424:1429	arg1	it					1447:1448	it	1447:1448	it	1447:1448	OBJECTIVE Our objective is to develop different compositions of chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity and architectures with the application of 3D printing and observe the growth of cartilage on it.
33334294	13	138	theme	composite	2366:2374	arg1	hydrogel					2386:2393	3D printed chitosan composite scaffolds hydrogel	2346:2393	3D printed chitosan composite scaffolds hydrogel	2346:2393	In the second part, the characterization of 3D printed chitosan composite scaffolds hydrogel is performed for evaluating the morphological, mechanical, and physical properties.
33334294	17	139	theme	cartilage	3202:3210	arg1	formation					3212:3220	the cartilage formation	3198:3220	the cartilage formation	3198:3220	The prepared scaffolds are highly porous, having optimum porosity, optimal mechanical strength to sustain the cartilage formation.
33334294	4	140	theme	ear	456:458	arg1	septum					467:472	the ear, nasal septum	452:472	the ear, nasal septum in the form of hyaline cartilage	452:505	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	11	141	theme	hydrogel	1865:1872	arg1	development					1814:1824	the development	1810:1824	the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels	1810:2019	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	7	142	theme	Laser-assisted	1001:1014	arg1	bioprinting					988:998	3D bioprinting	985:998	3D bioprinting	985:998	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	7	142	theme	Laser-assisted	1001:1014	arg1	LAB					1032:1034	LAB	1032:1034	LAB	1032:1034	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	7	142	theme	Laser-assisted	1001:1014	arg1	Bioprinting					1019:1029	Laser-assisted 3D Bioprinting	1001:1029	Laser-assisted 3D Bioprinting (LAB)	1001:1035	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	17	143	dep	strength	3178:3185	arg1	sustain					3190:3196	sustain	3190:3196	to sustain the cartilage formation	3187:3220	The prepared scaffolds are highly porous, having optimum porosity, optimal mechanical strength to sustain the cartilage formation.
33334294	11	144	theme	printed	1984:1990	arg1	hydrogels					2011:2019	3D printed chitosan composite hydrogels	1981:2019	3D printed chitosan composite hydrogels	1981:2019	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	7	145	theme	3D	985:986	arg1	combination					1056:1066	stereolithography combination	1038:1066	stereolithography combination with biomaterials	1038:1084	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	7	145	theme	3D	985:986	arg1	bioprinting					988:998	3D bioprinting	985:998	3D bioprinting	985:998	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	7	145	theme	3D	985:986	arg1	Bioprinting					1019:1029	Laser-assisted 3D Bioprinting	1001:1029	Laser-assisted 3D Bioprinting (LAB)	1001:1035	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	1	146	theme	printed	103:109	arg1	scaffolds					137:145	3D printed chitosan-gelatin-alginate scaffolds	100:145	3D printed chitosan-gelatin-alginate scaffolds	100:145	AIMS Our aim is to develop 3D printed chitosan-gelatin-alginate scaffolds using a costeffective in house designed 3D printer followed by its characterization.
33334294	20	147	theme	3D	3576:3577	arg1	scaffold					3596:3603	3D printed chitosan scaffold	3576:3603	3D printed chitosan scaffold	3576:3603	CONCLUSION This method helps in regeneration of degenerated cartilage for patient-specific and form above experiment we also concluded that 3D printed chitosan scaffold is best suited for the regeneration of chondrocyte cells.
33334294	6	148	theme	tissue	893:898	arg1	regeneration					900:911	cartilage tissue regeneration	883:911	cartilage tissue regeneration	883:911	3D printing plays a crucial role in the development of controlled porous architectures of scaffolds for cartilage tissue regeneration.
33334294	11	149	theme	chitosan-alginate-gelatin	1829:1853	arg1	hydrogel					1865:1872	chitosan-alginate-gelatin composite hydrogel	1829:1872	chitosan-alginate-gelatin composite hydrogel	1829:1872	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	7	150	theme	printing	932:939	arg1	technology					941:950	3D printing technology	929:950	3D printing technology	929:950	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	9	151	theme	paste	1490:1494	arg1	printer					1505:1511	an in-house 3D paste extruder printer	1475:1511	an in-house 3D paste extruder printer	1475:1511	To achieve as proposed, an in-house 3D paste extruder printer was developed, which is capable of printing porous composite chitosan hydrogel scaffolds of desired architecture layer by layer.
33334294	15	152	located	observed	2771:2778	arg2	differentiation					2729:2743	the differentiation	2725:2743	the differentiation of chondrocytes cells	2725:2765	In the last part of the study, the differentiation of chondrocytes cells was observed with human Mesenchymal Stem Cells (hMSC) on 3D printed scaffolds and showed positive results for the same.
33334294	15	152	located	observed	2771:2778	arg1	scaffolds					2835:2843	3D printed scaffolds	2824:2843	3D printed scaffolds	2824:2843	In the last part of the study, the differentiation of chondrocytes cells was observed with human Mesenchymal Stem Cells (hMSC) on 3D printed scaffolds and showed positive results for the same.
33334294	15	152	located	observed	2771:2778	arg1	part					2706:2709	the last part	2697:2709	the last part of the study	2697:2722	In the last part of the study, the differentiation of chondrocytes cells was observed with human Mesenchymal Stem Cells (hMSC) on 3D printed scaffolds and showed positive results for the same.
33334294	14	153	theme	mechanical	2659:2668	arg1	testing					2670:2676	mechanical testing	2659:2676	mechanical testing	2659:2676	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	14	153	theme	mechanical	2659:2668	arg1	Spectroscopy					2561:2572	Infrared Spectroscopy	2552:2572	Infrared Spectroscopy (FTIR)	2552:2579	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	18	154	theme	chondrocytes	3308:3319	arg1	differentiation					3289:3303	the differentiation	3285:3303	the differentiation of chondrocytes	3285:3319	The 3D printed chitosan composite scaffolds show supports for the differentiation of chondrocytes.
33334294	11	155	from	observation	1924:1934	arg1	hydrogels					2011:2019	3D printed chitosan composite hydrogels	1981:2019	3D printed chitosan composite hydrogels	1981:2019	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	8	156	theme	chitosan-gelatin-alginate	1274:1298	arg1	scaffolds					1319:1327	chitosan-gelatin-alginate composite hydrogel scaffolds	1274:1327	chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity	1274:1352	OBJECTIVE Our objective is to develop different compositions of chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity and architectures with the application of 3D printing and observe the growth of cartilage on it.
33334294	8	157	with	architectures	1358:1370	arg1	application					1381:1391	the application	1377:1391	the application of 3D printing	1377:1406	OBJECTIVE Our objective is to develop different compositions of chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity and architectures with the application of 3D printing and observe the growth of cartilage on it.
33334294	8	157	with	architectures	1358:1370	arg1	porosity					1345:1352	controlled porosity	1334:1352	controlled porosity	1334:1352	OBJECTIVE Our objective is to develop different compositions of chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity and architectures with the application of 3D printing and observe the growth of cartilage on it.
33334294	7	158	theme	period	1196:1201	arg1	frame					1203:1207	a short period frame	1188:1207	a short period frame	1188:1207	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	2	159	theme	3D	274:275	arg1	scaffolds					285:293	3D printed scaffolds	274:293	3D printed scaffolds as part of scaffold application	274:325	To observe chondrocyte differentiation on 3D printed scaffolds as part of scaffold application.
33334294	14	160	dep	Fourier	2534:2540	arg1	Transform					2542:2550	Transform	2542:2550	Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc	2542:2691	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	8	161	theme	hydrogel	1310:1317	arg1	scaffolds					1319:1327	chitosan-gelatin-alginate composite hydrogel scaffolds	1274:1327	chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity	1274:1352	OBJECTIVE Our objective is to develop different compositions of chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity and architectures with the application of 3D printing and observe the growth of cartilage on it.
33334294	15	162	theme	study	2718:2722	arg1	part					2706:2709	the last part	2697:2709	the last part of the study	2697:2722	In the last part of the study, the differentiation of chondrocytes cells was observed with human Mesenchymal Stem Cells (hMSC) on 3D printed scaffolds and showed positive results for the same.
33334294	20	163	theme	chondrocyte	3644:3654	arg1	cells					3656:3660	chondrocyte cells	3644:3660	chondrocyte cells	3644:3660	CONCLUSION This method helps in regeneration of degenerated cartilage for patient-specific and form above experiment we also concluded that 3D printed chitosan scaffold is best suited for the regeneration of chondrocyte cells.
33334294	4	164	theme	hyaline	489:495	arg1	cartilage					497:505	hyaline cartilage	489:505	hyaline cartilage	489:505	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	19	165	theme	cartilage	3415:3423	arg1	disorders					3425:3433	related cartilage disorders	3407:3433	related cartilage disorders	3407:3433	The above study is helpful for in-vivo regeneration of cartilage for patients having related cartilage disorders.
33334294	4	166	theme	bones	653:657	arg1	septum					467:472	the ear, nasal septum	452:472	the ear, nasal septum in the form of hyaline cartilage	452:505	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	4	166	theme	bones	653:657	arg1	joints					643:648	various joints	635:648	various joints of bones	635:657	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	4	166	theme	bones	653:657	arg1	cage					434:437	the rib cage	426:437	the rib cage	426:437	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	4	166	theme	bones	653:657	arg1	lobe					444:447	the lobe	440:447	the lobe of the ear, nasal septum in the form of hyaline cartilage	440:505	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	11	167	theme	paper	1792:1796	arg1	process					1798:1804	present paper process	1784:1804	present paper process	1784:1804	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	5	168	theme	damaged	681:687	arg1	tissues					689:695	or repair damaged tissues	671:695	tissues	689:695	To replace or repair damaged tissues due to disorders or trauma, thousands of surgical procedures are performed daily.
33334294	1	169	from	house	172:176	arg1	costeffective					155:167	costeffective	155:167	costeffective	155:167	AIMS Our aim is to develop 3D printed chitosan-gelatin-alginate scaffolds using a costeffective in house designed 3D printer followed by its characterization.
33334294	18	170	theme	chitosan	3238:3245	arg1	scaffolds					3257:3265	The 3D printed chitosan composite scaffolds	3223:3265	The 3D printed chitosan composite scaffolds	3223:3265	The 3D printed chitosan composite scaffolds show supports for the differentiation of chondrocytes.
33334294	19	171	contain	having	3400:3405	arg2	disorders					3425:3433	related cartilage disorders	3407:3433	related cartilage disorders	3407:3433	The above study is helpful for in-vivo regeneration of cartilage for patients having related cartilage disorders.
33334294	19	171	contain	having	3400:3405	arg1	patients					3391:3398	patients	3391:3398	patients having related cartilage disorders	3391:3433	The above study is helpful for in-vivo regeneration of cartilage for patients having related cartilage disorders.
33334294	14	172	theme	Microscopy	2624:2633	arg1	SEM					2635:2637	Scanning Electron Microscopy SEM	2606:2637	Scanning Electron Microscopy SEM	2606:2637	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	14	172	theme	Microscopy	2624:2633	arg1	Spectroscopy					2561:2572	Infrared Spectroscopy	2552:2572	Infrared Spectroscopy (FTIR)	2552:2579	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	12	173	theme	composite	2281:2289	arg1	hydrogels					2291:2299	printing chitosan composite hydrogels	2263:2299	printing chitosan composite hydrogels	2263:2299	The present study is divided into three parts: in first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels.
33334294	10	174	theme	composite	1692:1700	arg1	scaffolds					1702:1710	3D printed chitosan composite scaffolds	1672:1710	3D printed chitosan composite scaffolds	1672:1710	After the characterization of 3D printed chitosan composite scaffolds, the differentiation of chondrocyte was observed using hMSC.
33334294	7	175	theme	cartilage	1165:1173	arg1	development					1120:1130	the quick development	1110:1130	the quick development of patient-specific articulating cartilage	1110:1173	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	1	176	from	costeffective	155:167	arg1	house					172:176	house	172:176	house	172:176	AIMS Our aim is to develop 3D printed chitosan-gelatin-alginate scaffolds using a costeffective in house designed 3D printer followed by its characterization.
33334294	18	177	theme	3D	3227:3228	arg1	scaffolds					3257:3265	The 3D printed chitosan composite scaffolds	3223:3265	The 3D printed chitosan composite scaffolds	3223:3265	The 3D printed chitosan composite scaffolds show supports for the differentiation of chondrocytes.
33334294	15	178	theme	human	2785:2789	arg1	hMSC					2815:2818	hMSC	2815:2818	hMSC	2815:2818	In the last part of the study, the differentiation of chondrocytes cells was observed with human Mesenchymal Stem Cells (hMSC) on 3D printed scaffolds and showed positive results for the same.
33334294	15	178	theme	human	2785:2789	arg1	Cells					2808:2812	human Mesenchymal Stem Cells	2785:2812	human Mesenchymal Stem Cells (hMSC)	2785:2819	In the last part of the study, the differentiation of chondrocytes cells was observed with human Mesenchymal Stem Cells (hMSC) on 3D printed scaffolds and showed positive results for the same.
33334294	3	179	theme	significant	371:381	arg1	tissue					383:388	a significant tissue	369:388	a significant tissue	369:388	BACKGROUND Cartilage is considered to be a significant tissue in humans.
33334294	3	179	theme	significant	371:381	arg1	Cartilage					339:347	BACKGROUND Cartilage	328:347	BACKGROUND Cartilage	328:347	BACKGROUND Cartilage is considered to be a significant tissue in humans.
33334294	8	180	theme	architectures	1358:1370	arg1	compositions					1258:1269	different compositions	1248:1269	different compositions of chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity and architectures with the application of 3D printing	1248:1406	OBJECTIVE Our objective is to develop different compositions of chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity and architectures with the application of 3D printing and observe the growth of cartilage on it.
33334294	15	181	theme	last	2701:2704	arg1	part					2706:2709	the last part	2697:2709	the last part of the study	2697:2722	In the last part of the study, the differentiation of chondrocytes cells was observed with human Mesenchymal Stem Cells (hMSC) on 3D printed scaffolds and showed positive results for the same.
33334294	0	182	theme	Printed	3:9	arg1	Scaffold					30:37	3D Printed Chitosan Composite Scaffold	0:37	3D Printed Chitosan Composite Scaffold for Chondrocytes Differentiation.	0:71	3D Printed Chitosan Composite Scaffold for Chondrocytes Differentiation.
33334294	4	183	dep	ribs	519:522	arg1	cartilage					531:539	costal cartilage	524:539	costal cartilage	524:539	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	12	184	from	utilization	2165:2175	arg1	printer					2234:2240	house customized assembled paste extruder based 3D printer	2183:2240	house customized assembled paste extruder based 3D printer	2183:2240	The present study is divided into three parts: in first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels.
33334294	9	185	theme	printing	1548:1555	arg1	scaffolds					1592:1600	printing porous composite chitosan hydrogel scaffolds	1548:1600	printing porous composite chitosan hydrogel scaffolds of desired architecture layer by layer	1548:1639	To achieve as proposed, an in-house 3D paste extruder printer was developed, which is capable of printing porous composite chitosan hydrogel scaffolds of desired architecture layer by layer.
33334294	20	186	theme	degenerated	3484:3494	arg1	cartilage					3496:3504	degenerated cartilage	3484:3504	degenerated cartilage	3484:3504	CONCLUSION This method helps in regeneration of degenerated cartilage for patient-specific and form above experiment we also concluded that 3D printed chitosan scaffold is best suited for the regeneration of chondrocyte cells.
33334294	4	187	theme	costal	524:529	arg1	cartilage					531:539	costal cartilage	524:539	costal cartilage	524:539	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	11	188	from	printing	1878:1885	arg1	hydrogels					2011:2019	3D printed chitosan composite hydrogels	1981:2019	3D printed chitosan composite hydrogels	1981:2019	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	18	189	dep	show	3267:3270	arg1	supports					3272:3279	supports	3272:3279	show supports for the differentiation of chondrocytes	3267:3319	The 3D printed chitosan composite scaffolds show supports for the differentiation of chondrocytes.
33334294	0	190	theme	Composite	20:28	arg1	Scaffold					30:37	3D Printed Chitosan Composite Scaffold	0:37	3D Printed Chitosan Composite Scaffold for Chondrocytes Differentiation.	0:71	3D Printed Chitosan Composite Scaffold for Chondrocytes Differentiation.
33334294	8	191	dep	OBJECTIVE	1210:1218	arg1	is					1234:1235	is	1234:1235	is to develop different compositions of chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity and architectures with the application of 3D printing and observe the growth of cartilage on it	1234:1448	OBJECTIVE Our objective is to develop different compositions of chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity and architectures with the application of 3D printing and observe the growth of cartilage on it.
33334294	12	192	theme	printing	2263:2270	arg1	hydrogels					2291:2299	printing chitosan composite hydrogels	2263:2299	printing chitosan composite hydrogels	2263:2299	The present study is divided into three parts: in first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels.
33334294	7	193	dep	inkjet	957:962	arg1	micro-					965:970	micro-	965:970	micro-	965:970	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	10	194	theme	printed	1675:1681	arg1	scaffolds					1702:1710	3D printed chitosan composite scaffolds	1672:1710	3D printed chitosan composite scaffolds	1672:1710	After the characterization of 3D printed chitosan composite scaffolds, the differentiation of chondrocyte was observed using hMSC.
33334294	15	195	theme	cells	2761:2765	arg1	differentiation					2729:2743	the differentiation	2725:2743	the differentiation of chondrocytes cells	2725:2765	In the last part of the study, the differentiation of chondrocytes cells was observed with human Mesenchymal Stem Cells (hMSC) on 3D printed scaffolds and showed positive results for the same.
33334294	9	196	theme	hydrogel	1583:1590	arg1	scaffolds					1592:1600	printing porous composite chitosan hydrogel scaffolds	1548:1600	printing porous composite chitosan hydrogel scaffolds of desired architecture layer by layer	1548:1639	To achieve as proposed, an in-house 3D paste extruder printer was developed, which is capable of printing porous composite chitosan hydrogel scaffolds of desired architecture layer by layer.
33334294	13	197	theme	3D	2346:2347	arg1	hydrogel					2386:2393	3D printed chitosan composite scaffolds hydrogel	2346:2393	3D printed chitosan composite scaffolds hydrogel	2346:2393	In the second part, the characterization of 3D printed chitosan composite scaffolds hydrogel is performed for evaluating the morphological, mechanical, and physical properties.
33334294	8	198	theme	printing	1399:1406	arg1	application					1381:1391	the application	1377:1391	the application of 3D printing	1377:1406	OBJECTIVE Our objective is to develop different compositions of chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity and architectures with the application of 3D printing and observe the growth of cartilage on it.
33334294	11	199	theme	cells	1972:1976	arg1	differentiation					1940:1954	differentiation	1940:1954	differentiation of chondrocytes cells	1940:1976	METHODS In present paper process for the development of chitosan-alginate-gelatin composite hydrogel, 3D printing, morphological characterization, and observation for differentiation of chondrocytes cells on 3D printed chitosan composite hydrogels is presented.
33334294	5	200	theme	surgical	738:745	arg1	procedures					747:756	surgical procedures	738:756	surgical procedures	738:756	To replace or repair damaged tissues due to disorders or trauma, thousands of surgical procedures are performed daily.
33334294	6	201	theme	crucial	799:805	arg1	role					807:810	a crucial role	797:810	a crucial role	797:810	3D printing plays a crucial role in the development of controlled porous architectures of scaffolds for cartilage tissue regeneration.
33334294	4	202	from	lobe	444:447	arg1	form					481:484	the form	477:484	the form of hyaline cartilage	477:505	It is present in between the rib cage, the lobe of the ear, nasal septum in the form of hyaline cartilage, in between ribs costal cartilage, intervertebral discs in the form of fibrocartilage, meniscus, larynx, epiglottis and between various joints of bones.
33334294	17	203	theme	optimum	3141:3147	arg1	porosity					3149:3156	optimum porosity	3141:3156	optimum porosity	3141:3156	The prepared scaffolds are highly porous, having optimum porosity, optimal mechanical strength to sustain the cartilage formation.
33334294	17	203	theme	optimum	3141:3147	arg1	strength					3178:3185	optimal mechanical strength	3159:3185	optimal mechanical strength to sustain the cartilage formation	3159:3220	The prepared scaffolds are highly porous, having optimum porosity, optimal mechanical strength to sustain the cartilage formation.
33334294	12	204	theme	based	2225:2229	arg1	printer					2234:2240	house customized assembled paste extruder based 3D printer	2183:2240	house customized assembled paste extruder based 3D printer	2183:2240	The present study is divided into three parts: in first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels.
33334294	9	205	theme	architecture	1613:1624	arg1	layer					1626:1630	desired architecture layer	1605:1630	desired architecture layer by layer	1605:1639	To achieve as proposed, an in-house 3D paste extruder printer was developed, which is capable of printing porous composite chitosan hydrogel scaffolds of desired architecture layer by layer.
33334294	7	206	with	combination	1056:1066	arg1	biomaterials					1073:1084	biomaterials	1073:1084	biomaterials	1073:1084	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	8	207	with	scaffolds	1319:1327	arg1	application					1381:1391	the application	1377:1391	the application of 3D printing	1377:1406	OBJECTIVE Our objective is to develop different compositions of chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity and architectures with the application of 3D printing and observe the growth of cartilage on it.
33334294	8	207	with	scaffolds	1319:1327	arg1	porosity					1345:1352	controlled porosity	1334:1352	controlled porosity	1334:1352	OBJECTIVE Our objective is to develop different compositions of chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity and architectures with the application of 3D printing and observe the growth of cartilage on it.
33334294	12	208	theme	paste	2210:2214	arg1	printer					2234:2240	house customized assembled paste extruder based 3D printer	2183:2240	house customized assembled paste extruder based 3D printer	2183:2240	The present study is divided into three parts: in first part development of composite chitosan-alginate-gelatin hydrogel with the utilization of in house customized assembled paste extruder based 3D printer, which is capable of printing chitosan composite hydrogels.
33334294	14	209	theme	prepared	2483:2490	arg1	scaffolds					2502:2510	The prepared composite scaffolds	2479:2510	The prepared composite scaffolds	2479:2510	The prepared composite scaffolds were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction(XRD), Scanning Electron Microscopy SEM, swelling property, mechanical testing, porosity, etc.
33334294	10	210	theme	chondrocyte	1736:1746	arg1	differentiation					1717:1731	the differentiation	1713:1731	the differentiation of chondrocyte	1713:1746	After the characterization of 3D printed chitosan composite scaffolds, the differentiation of chondrocyte was observed using hMSC.
33334294	6	211	theme	3D	779:780	arg1	printing					782:789	3D printing	779:789	3D printing	779:789	3D printing plays a crucial role in the development of controlled porous architectures of scaffolds for cartilage tissue regeneration.
33334294	13	212	theme	morphological	2427:2439	arg1	properties					2467:2476	the morphological, mechanical, and physical properties	2423:2476	the morphological, mechanical, and physical properties	2423:2476	In the second part, the characterization of 3D printed chitosan composite scaffolds hydrogel is performed for evaluating the morphological, mechanical, and physical properties.
33334294	8	213	theme	cartilage	1434:1442	arg1	growth					1424:1429	the growth	1420:1429	the growth of cartilage on it	1420:1448	OBJECTIVE Our objective is to develop different compositions of chitosan-gelatin-alginate composite hydrogel scaffolds with controlled porosity and architectures with the application of 3D printing and observe the growth of cartilage on it.
33334294	7	214	theme	patient-specific	1135:1150	arg1	cartilage					1165:1173	patient-specific articulating cartilage	1135:1173	patient-specific articulating cartilage	1135:1173	Advancement in 3D printing technology like inkjet, micro- extrusion in 3D bioprinting, Laser-assisted 3D Bioprinting (LAB), stereolithography combination with biomaterials plays a crucial role in the quick development of patient-specific articulating cartilage when need in a short period frame.
33334294	19	215	theme	above	3326:3330	arg1	helpful					3341:3347	helpful	3341:3347	helpful	3341:3347	The above study is helpful for in-vivo regeneration of cartilage for patients having related cartilage disorders.
33334294	19	215	theme	above	3326:3330	arg1	study					3332:3336	The above study	3322:3336	The above study	3322:3336	The above study is helpful for in-vivo regeneration of cartilage for patients having related cartilage disorders.
33480038	0	0	theme	UHPLC-QTRAP-MS/MS	89:105	arg1	method					107:112	an efficient UHPLC-QTRAP-MS/MS method	76:112	an efficient UHPLC-QTRAP-MS/MS method	76:112	Determination of monosaccharides in Lycium barbarum fruit polysaccharide by an efficient UHPLC-QTRAP-MS/MS method.
33480038	5	1	theme	comprehensive	832:844	arg1	evaluation					846:855	comprehensive evaluation	832:855	comprehensive evaluation of the samples	832:870	Principal component analysis (PCA) and technique for order of preference by similarity to ideal solution (TOPSIS) analysis were used for comprehensive evaluation of the samples.
33480038	10	2	theme	pre-column	1567:1576	arg1	simple					1623:1628	simple	1623:1628	simple	1623:1628	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	10	2	theme	pre-column	1567:1576	arg1	method					1593:1598	The UHPLC-QTRAP-MS/MS pre-column derivatisation method	1545:1598	The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study	1545:1617	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	10	3	theme	origin	1724:1729	arg1	distinction					1731:1741	origin distinction	1724:1741	origin distinction	1724:1741	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	0	4	theme	efficient	79:87	arg1	method					107:112	an efficient UHPLC-QTRAP-MS/MS method	76:112	an efficient UHPLC-QTRAP-MS/MS method	76:112	Determination of monosaccharides in Lycium barbarum fruit polysaccharide by an efficient UHPLC-QTRAP-MS/MS method.
33480038	9	5	theme	rapid	1224:1228	arg1	method					1253:1258	A rapid quantitative detection method	1222:1258	A rapid quantitative detection method	1222:1258	RESULTS A rapid quantitative detection method was established, in which the hydrolysis time was reduced from 12 h to 2 h. By using LBP as one of the indexes to evaluate the quality of L. barbarum, L. barbarum from Zhongning County, Ningxia Province, was identified as having the best quality among the varieties tested.
33480038	0	6	from	Determination	0:12	arg1	polysaccharide					58:71	Lycium barbarum fruit polysaccharide	36:71	Lycium barbarum fruit polysaccharide	36:71	Determination of monosaccharides in Lycium barbarum fruit polysaccharide by an efficient UHPLC-QTRAP-MS/MS method.
33480038	7	7	theme	linear	1014:1019	arg1	relationship					1021:1032	The linear relationship	1010:1032	The linear relationship of the seven monosaccharides	1010:1061	The linear relationship of the seven monosaccharides was optimum within a certain concentration range.
33480038	7	7	theme	linear	1014:1019	arg1	optimum					1067:1073	optimum	1067:1073	optimum	1067:1073	The linear relationship of the seven monosaccharides was optimum within a certain concentration range.
33480038	10	8	theme	other	1762:1766	arg1	plants					1778:1783	other medicinal plants	1762:1783	other medicinal plants	1762:1783	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	10	9	theme	medicinal	1768:1776	arg1	plants					1778:1783	other medicinal plants	1762:1783	other medicinal plants	1762:1783	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	4	10	theme	barbarum	678:685	arg1	fruits					687:692	L. barbarum fruits	675:692	L. barbarum fruits	675:692	METHODS In our study, hydrochloric acid was used to hydrolyse the polysaccharide obtained from L. barbarum fruits.
33480038	5	11	theme	Principal	695:703	arg1	PCA					725:727	PCA	725:727	PCA	725:727	Principal component analysis (PCA) and technique for order of preference by similarity to ideal solution (TOPSIS) analysis were used for comprehensive evaluation of the samples.
33480038	5	11	theme	Principal	695:703	arg1	analysis					715:722	Principal component analysis	695:722	Principal component analysis (PCA)	695:728	Principal component analysis (PCA) and technique for order of preference by similarity to ideal solution (TOPSIS) analysis were used for comprehensive evaluation of the samples.
33480038	4	12	theme	L.	675:676	arg1	fruits					687:692	L. barbarum fruits	675:692	L. barbarum fruits	675:692	METHODS In our study, hydrochloric acid was used to hydrolyse the polysaccharide obtained from L. barbarum fruits.
33480038	8	13	theme	Quantitative	1113:1124	arg1	recoveries					1126:1135	Quantitative recoveries	1113:1135	Quantitative recoveries of the seven monosaccharides from the samples	1113:1181	Quantitative recoveries of the seven monosaccharides from the samples ranged from 94.76% to 102.11%.
33480038	5	14	theme	component	705:713	arg1	PCA					725:727	PCA	725:727	PCA	725:727	Principal component analysis (PCA) and technique for order of preference by similarity to ideal solution (TOPSIS) analysis were used for comprehensive evaluation of the samples.
33480038	5	14	theme	component	705:713	arg1	analysis					715:722	Principal component analysis	695:722	Principal component analysis (PCA)	695:728	Principal component analysis (PCA) and technique for order of preference by similarity to ideal solution (TOPSIS) analysis were used for comprehensive evaluation of the samples.
33480038	2	15	theme	spectrometry	342:353	arg1	method					375:380	a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method	258:380	a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method	258:380	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	3	16	theme	OBJECTIVE	498:506	arg1	Exploration					508:518	OBJECTIVE Exploration	498:518	OBJECTIVE Exploration of a new efficient method for monosaccharide determination	498:577	OBJECTIVE Exploration of a new efficient method for monosaccharide determination.
33480038	1	17	theme	common	202:207	arg1	derivatisation					139:152	INTRODUCTION Pre-column derivatisation	115:152	INTRODUCTION Pre-column derivatisation using 1-phenyl-3-methyl-5-pyrazolone (PMP)	115:195	INTRODUCTION Pre-column derivatisation using 1-phenyl-3-methyl-5-pyrazolone (PMP) is a common method for monosaccharide determination.
33480038	1	17	theme	common	202:207	arg1	method					209:214	a common method	200:214	a common method for monosaccharide determination	200:247	INTRODUCTION Pre-column derivatisation using 1-phenyl-3-methyl-5-pyrazolone (PMP) is a common method for monosaccharide determination.
33480038	4	18	used	used	624:627	arg2	acid					615:618	hydrochloric acid	602:618	hydrochloric acid	602:618	METHODS In our study, hydrochloric acid was used to hydrolyse the polysaccharide obtained from L. barbarum fruits.
33480038	2	19	theme	mass	337:340	arg1	UHPLC-QTRAP-MS/MS					356:372	UHPLC-QTRAP-MS/MS	356:372	UHPLC-QTRAP-MS/MS	356:372	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	2	19	theme	mass	337:340	arg1	spectrometry					342:353	a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry	258:353	a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method	258:380	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	10	20	theme	good	1661:1664	arg1	repeatability					1666:1678	good repeatability	1661:1678	good repeatability	1661:1678	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	10	21	theme	barbarum	1749:1756	arg1	evaluation					1709:1718	quality evaluation	1701:1718	quality evaluation	1701:1718	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	10	21	theme	barbarum	1749:1756	arg1	distinction					1731:1741	origin distinction	1724:1741	origin distinction	1724:1741	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	8	22	dep	%	1200:1200	arg1	to					1202:1203	to	1202:1203	to	1202:1203	Quantitative recoveries of the seven monosaccharides from the samples ranged from 94.76% to 102.11%.
33480038	2	23	used	used	400:403	arg2	method					375:380	a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method	258:380	a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method	258:380	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	2	24	theme	Lycium	460:465	arg1	LBP					492:494	LBP	492:494	LBP	492:494	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	2	24	theme	Lycium	460:465	arg1	polysaccharide					476:489	Lycium barbarum polysaccharide	460:489	Lycium barbarum polysaccharide (LBP)	460:495	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	4	25	dep	METHODS	580:586	arg1	study					595:599	our study	591:599	our study	591:599	METHODS In our study, hydrochloric acid was used to hydrolyse the polysaccharide obtained from L. barbarum fruits.
33480038	5	26	used	used	823:826	arg2	analysis					715:722	Principal component analysis	695:722	Principal component analysis (PCA)	695:728	Principal component analysis (PCA) and technique for order of preference by similarity to ideal solution (TOPSIS) analysis were used for comprehensive evaluation of the samples.
33480038	5	26	used	used	823:826	arg2	technique					734:742	technique	734:742	technique for order of preference by similarity to ideal solution (TOPSIS)	734:807	Principal component analysis (PCA) and technique for order of preference by similarity to ideal solution (TOPSIS) analysis were used for comprehensive evaluation of the samples.
33480038	5	26	used	used	823:826	arg2	PCA					725:727	PCA	725:727	PCA	725:727	Principal component analysis (PCA) and technique for order of preference by similarity to ideal solution (TOPSIS) analysis were used for comprehensive evaluation of the samples.
33480038	8	27	theme	102.11	1205:1210	arg1	%					1200:1200	%	1200:1200	%	1200:1200	Quantitative recoveries of the seven monosaccharides from the samples ranged from 94.76% to 102.11%.
33480038	1	28	theme	monosaccharide	220:233	arg1	determination					235:247	monosaccharide determination	220:247	monosaccharide determination	220:247	INTRODUCTION Pre-column derivatisation using 1-phenyl-3-methyl-5-pyrazolone (PMP) is a common method for monosaccharide determination.
33480038	9	29	theme	detection	1243:1251	arg1	method					1253:1258	A rapid quantitative detection method	1222:1258	A rapid quantitative detection method	1222:1258	RESULTS A rapid quantitative detection method was established, in which the hydrolysis time was reduced from 12 h to 2 h. By using LBP as one of the indexes to evaluate the quality of L. barbarum, L. barbarum from Zhongning County, Ningxia Province, was identified as having the best quality among the varieties tested.
33480038	9	30	theme	indexes	1363:1369	arg1	L.					1411:1412	L.	1411:1412	L.	1411:1412	RESULTS A rapid quantitative detection method was established, in which the hydrolysis time was reduced from 12 h to 2 h. By using LBP as one of the indexes to evaluate the quality of L. barbarum, L. barbarum from Zhongning County, Ningxia Province, was identified as having the best quality among the varieties tested.
33480038	9	30	theme	indexes	1363:1369	arg1	one					1352:1354	one	1352:1354	one	1352:1354	RESULTS A rapid quantitative detection method was established, in which the hydrolysis time was reduced from 12 h to 2 h. By using LBP as one of the indexes to evaluate the quality of L. barbarum, L. barbarum from Zhongning County, Ningxia Province, was identified as having the best quality among the varieties tested.
33480038	9	30	theme	indexes	1363:1369	arg1	indexes					1363:1369	the indexes	1359:1369	the indexes to evaluate the quality of L. barbarum	1359:1408	RESULTS A rapid quantitative detection method was established, in which the hydrolysis time was reduced from 12 h to 2 h. By using LBP as one of the indexes to evaluate the quality of L. barbarum, L. barbarum from Zhongning County, Ningxia Province, was identified as having the best quality among the varieties tested.
33480038	1	31	theme	INTRODUCTION	115:126	arg1	derivatisation					139:152	INTRODUCTION Pre-column derivatisation	115:152	INTRODUCTION Pre-column derivatisation using 1-phenyl-3-methyl-5-pyrazolone (PMP)	115:195	INTRODUCTION Pre-column derivatisation using 1-phenyl-3-methyl-5-pyrazolone (PMP) is a common method for monosaccharide determination.
33480038	1	31	theme	INTRODUCTION	115:126	arg1	method					209:214	a common method	200:214	a common method for monosaccharide determination	200:247	INTRODUCTION Pre-column derivatisation using 1-phenyl-3-methyl-5-pyrazolone (PMP) is a common method for monosaccharide determination.
33480038	3	32	theme	method	539:544	arg1	Exploration					508:518	OBJECTIVE Exploration	498:518	OBJECTIVE Exploration of a new efficient method for monosaccharide determination	498:577	OBJECTIVE Exploration of a new efficient method for monosaccharide determination.
33480038	0	33	theme	monosaccharides	17:31	arg1	Determination					0:12	Determination	0:12	Determination of monosaccharides in Lycium barbarum fruit polysaccharide by an efficient UHPLC-QTRAP-MS/MS method.	0:113	Determination of monosaccharides in Lycium barbarum fruit polysaccharide by an efficient UHPLC-QTRAP-MS/MS method.
33480038	5	34	theme	ideal	785:789	arg1	TOPSIS					801:806	TOPSIS	801:806	TOPSIS	801:806	Principal component analysis (PCA) and technique for order of preference by similarity to ideal solution (TOPSIS) analysis were used for comprehensive evaluation of the samples.
33480038	5	34	theme	ideal	785:789	arg1	solution					791:798	ideal solution	785:798	ideal solution (TOPSIS)	785:807	Principal component analysis (PCA) and technique for order of preference by similarity to ideal solution (TOPSIS) analysis were used for comprehensive evaluation of the samples.
33480038	9	35	dep	L.	1411:1412	arg1	barbarum					1414:1421	L. barbarum from Zhongning County, Ningxia Province	1411:1461	L. barbarum from Zhongning County, Ningxia Province	1411:1461	RESULTS A rapid quantitative detection method was established, in which the hydrolysis time was reduced from 12 h to 2 h. By using LBP as one of the indexes to evaluate the quality of L. barbarum, L. barbarum from Zhongning County, Ningxia Province, was identified as having the best quality among the varieties tested.
33480038	1	36	theme	Pre-column	128:137	arg1	derivatisation					139:152	INTRODUCTION Pre-column derivatisation	115:152	INTRODUCTION Pre-column derivatisation using 1-phenyl-3-methyl-5-pyrazolone (PMP)	115:195	INTRODUCTION Pre-column derivatisation using 1-phenyl-3-methyl-5-pyrazolone (PMP) is a common method for monosaccharide determination.
33480038	1	36	theme	Pre-column	128:137	arg1	method					209:214	a common method	200:214	a common method for monosaccharide determination	200:247	INTRODUCTION Pre-column derivatisation using 1-phenyl-3-methyl-5-pyrazolone (PMP) is a common method for monosaccharide determination.
33480038	9	37	dep	RESULTS	1214:1220	arg1	established					1264:1274	established	1264:1274	established	1264:1274	RESULTS A rapid quantitative detection method was established, in which the hydrolysis time was reduced from 12 h to 2 h. By using LBP as one of the indexes to evaluate the quality of L. barbarum, L. barbarum from Zhongning County, Ningxia Province, was identified as having the best quality among the varieties tested.
33480038	10	38	theme	plants	1778:1783	arg1	evaluation					1709:1718	quality evaluation	1701:1718	quality evaluation	1701:1718	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	10	38	theme	plants	1778:1783	arg1	distinction					1731:1741	origin distinction	1724:1741	origin distinction	1724:1741	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	2	39	theme	liquid	292:297	arg1	UHPLC-QTRAP-MS/MS					356:372	UHPLC-QTRAP-MS/MS	356:372	UHPLC-QTRAP-MS/MS	356:372	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	2	39	theme	liquid	292:297	arg1	spectrometry					342:353	a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry	258:353	a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method	258:380	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	3	40	theme	new	525:527	arg1	method					539:544	a new efficient method	523:544	a new efficient method for monosaccharide determination	523:577	OBJECTIVE Exploration of a new efficient method for monosaccharide determination.
33480038	2	41	theme	polysaccharide	476:489	arg1	composition					445:455	monosaccharide composition	430:455	monosaccharide composition of Lycium barbarum polysaccharide (LBP)	430:495	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	6	42	dep	monosaccharides	923:937	arg1	rhamnose					971:978	rhamnose	971:978	rhamnose	971:978	The results showed that LBP was composed of seven monosaccharides: galactose, arabinose, mannose, rhamnose, xylose, ribose, and glucose.
33480038	6	42	dep	monosaccharides	923:937	arg1	glucose					1001:1007	glucose	1001:1007	glucose	1001:1007	The results showed that LBP was composed of seven monosaccharides: galactose, arabinose, mannose, rhamnose, xylose, ribose, and glucose.
33480038	6	42	dep	monosaccharides	923:937	arg1	xylose					981:986	xylose	981:986	xylose	981:986	The results showed that LBP was composed of seven monosaccharides: galactose, arabinose, mannose, rhamnose, xylose, ribose, and glucose.
33480038	6	42	dep	monosaccharides	923:937	arg1	arabinose					951:959	arabinose	951:959	arabinose	951:959	The results showed that LBP was composed of seven monosaccharides: galactose, arabinose, mannose, rhamnose, xylose, ribose, and glucose.
33480038	6	42	dep	monosaccharides	923:937	arg1	mannose					962:968	mannose	962:968	mannose	962:968	The results showed that LBP was composed of seven monosaccharides: galactose, arabinose, mannose, rhamnose, xylose, ribose, and glucose.
33480038	6	42	dep	monosaccharides	923:937	arg1	ribose					989:994	ribose	989:994	ribose	989:994	The results showed that LBP was composed of seven monosaccharides: galactose, arabinose, mannose, rhamnose, xylose, ribose, and glucose.
33480038	6	42	dep	monosaccharides	923:937	arg1	monosaccharides					923:937	seven monosaccharides	917:937	seven monosaccharides: galactose, arabinose, mannose, rhamnose, xylose, ribose, and glucose	917:1007	The results showed that LBP was composed of seven monosaccharides: galactose, arabinose, mannose, rhamnose, xylose, ribose, and glucose.
33480038	6	42	dep	monosaccharides	923:937	arg1	galactose					940:948	galactose	940:948	galactose	940:948	The results showed that LBP was composed of seven monosaccharides: galactose, arabinose, mannose, rhamnose, xylose, ribose, and glucose.
33480038	0	43	theme	Lycium	36:41	arg1	polysaccharide					58:71	Lycium barbarum fruit polysaccharide	36:71	Lycium barbarum fruit polysaccharide	36:71	Determination of monosaccharides in Lycium barbarum fruit polysaccharide by an efficient UHPLC-QTRAP-MS/MS method.
33480038	9	44	theme	hydrolysis	1290:1299	arg1	time					1301:1304	the hydrolysis time	1286:1304	the hydrolysis time	1286:1304	RESULTS A rapid quantitative detection method was established, in which the hydrolysis time was reduced from 12 h to 2 h. By using LBP as one of the indexes to evaluate the quality of L. barbarum, L. barbarum from Zhongning County, Ningxia Province, was identified as having the best quality among the varieties tested.
33480038	2	45	theme	ultra-high-performance	269:290	arg1	UHPLC-QTRAP-MS/MS					356:372	UHPLC-QTRAP-MS/MS	356:372	UHPLC-QTRAP-MS/MS	356:372	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	2	45	theme	ultra-high-performance	269:290	arg1	spectrometry					342:353	a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry	258:353	a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method	258:380	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	3	46	theme	efficient	529:537	arg1	method					539:544	a new efficient method	523:544	a new efficient method for monosaccharide determination	523:577	OBJECTIVE Exploration of a new efficient method for monosaccharide determination.
33480038	5	47	theme	samples	864:870	arg1	evaluation					846:855	comprehensive evaluation	832:855	comprehensive evaluation of the samples	832:870	Principal component analysis (PCA) and technique for order of preference by similarity to ideal solution (TOPSIS) analysis were used for comprehensive evaluation of the samples.
33480038	4	48	theme	hydrochloric	602:613	arg1	acid					615:618	hydrochloric acid	602:618	hydrochloric acid	602:618	METHODS In our study, hydrochloric acid was used to hydrolyse the polysaccharide obtained from L. barbarum fruits.
33480038	7	49	theme	concentration	1092:1104	arg1	range					1106:1110	a certain concentration range	1082:1110	a certain concentration range	1082:1110	The linear relationship of the seven monosaccharides was optimum within a certain concentration range.
33480038	8	50	theme	94.76	1195:1199	arg1	%					1200:1200	%	1200:1200	%	1200:1200	Quantitative recoveries of the seven monosaccharides from the samples ranged from 94.76% to 102.11%.
33480038	2	51	theme	specific	260:267	arg1	UHPLC-QTRAP-MS/MS					356:372	UHPLC-QTRAP-MS/MS	356:372	UHPLC-QTRAP-MS/MS	356:372	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	2	51	theme	specific	260:267	arg1	spectrometry					342:353	a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry	258:353	a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method	258:380	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	10	52	used	used	1692:1695	arg2	method					1593:1598	The UHPLC-QTRAP-MS/MS pre-column derivatisation method	1545:1598	The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study	1545:1617	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	10	52	used	used	1692:1695	arg2	simple					1623:1628	simple	1623:1628	simple	1623:1628	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	9	53	theme	best	1493:1496	arg1	quality					1498:1504	the best quality	1489:1504	the best quality among the varieties tested	1489:1531	RESULTS A rapid quantitative detection method was established, in which the hydrolysis time was reduced from 12 h to 2 h. By using LBP as one of the indexes to evaluate the quality of L. barbarum, L. barbarum from Zhongning County, Ningxia Province, was identified as having the best quality among the varieties tested.
33480038	0	54	theme	fruit	52:56	arg1	polysaccharide					58:71	Lycium barbarum fruit polysaccharide	36:71	Lycium barbarum fruit polysaccharide	36:71	Determination of monosaccharides in Lycium barbarum fruit polysaccharide by an efficient UHPLC-QTRAP-MS/MS method.
33480038	5	55	theme	preference	757:766	arg1	order					748:752	order	748:752	order of preference	748:766	Principal component analysis (PCA) and technique for order of preference by similarity to ideal solution (TOPSIS) analysis were used for comprehensive evaluation of the samples.
33480038	10	56	theme	UHPLC-QTRAP-MS/MS	1549:1565	arg1	simple					1623:1628	simple	1623:1628	simple	1623:1628	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	10	56	theme	UHPLC-QTRAP-MS/MS	1549:1565	arg1	method					1593:1598	The UHPLC-QTRAP-MS/MS pre-column derivatisation method	1545:1598	The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study	1545:1617	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	0	57	dep	Lycium	36:41	arg1	barbarum					43:50	barbarum	43:50	barbarum	43:50	Determination of monosaccharides in Lycium barbarum fruit polysaccharide by an efficient UHPLC-QTRAP-MS/MS method.
33480038	7	58	theme	monosaccharides	1047:1061	arg1	relationship					1021:1032	The linear relationship	1010:1032	The linear relationship of the seven monosaccharides	1010:1061	The linear relationship of the seven monosaccharides was optimum within a certain concentration range.
33480038	7	58	theme	monosaccharides	1047:1061	arg1	optimum					1067:1073	optimum	1067:1073	optimum	1067:1073	The linear relationship of the seven monosaccharides was optimum within a certain concentration range.
33480038	10	59	theme	quality	1701:1707	arg1	evaluation					1709:1718	quality evaluation	1701:1718	quality evaluation	1701:1718	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	2	60	dep	Lycium	460:465	arg1	barbarum					467:474	barbarum	467:474	barbarum	467:474	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	2	61	theme	tandem	330:335	arg1	UHPLC-QTRAP-MS/MS					356:372	UHPLC-QTRAP-MS/MS	356:372	UHPLC-QTRAP-MS/MS	356:372	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	2	61	theme	tandem	330:335	arg1	spectrometry					342:353	a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry	258:353	a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method	258:380	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	9	62	theme	quantitative	1230:1241	arg1	method					1253:1258	A rapid quantitative detection method	1222:1258	A rapid quantitative detection method	1222:1258	RESULTS A rapid quantitative detection method was established, in which the hydrolysis time was reduced from 12 h to 2 h. By using LBP as one of the indexes to evaluate the quality of L. barbarum, L. barbarum from Zhongning County, Ningxia Province, was identified as having the best quality among the varieties tested.
33480038	3	63	theme	monosaccharide	550:563	arg1	determination					565:577	monosaccharide determination	550:577	monosaccharide determination	550:577	OBJECTIVE Exploration of a new efficient method for monosaccharide determination.
33480038	9	64	theme	barbarum	1401:1408	arg1	quality					1387:1393	the quality	1383:1393	the quality of L. barbarum	1383:1408	RESULTS A rapid quantitative detection method was established, in which the hydrolysis time was reduced from 12 h to 2 h. By using LBP as one of the indexes to evaluate the quality of L. barbarum, L. barbarum from Zhongning County, Ningxia Province, was identified as having the best quality among the varieties tested.
33480038	8	65	from	samples	1175:1181	arg1	monosaccharides					1150:1164	the seven monosaccharides	1140:1164	the seven monosaccharides from the samples	1140:1181	Quantitative recoveries of the seven monosaccharides from the samples ranged from 94.76% to 102.11%.
33480038	8	65	from	samples	1175:1181	arg1	recoveries					1126:1135	Quantitative recoveries	1113:1135	Quantitative recoveries of the seven monosaccharides from the samples	1113:1181	Quantitative recoveries of the seven monosaccharides from the samples ranged from 94.76% to 102.11%.
33480038	7	66	theme	certain	1084:1090	arg1	range					1106:1110	a certain concentration range	1082:1110	a certain concentration range	1082:1110	The linear relationship of the seven monosaccharides was optimum within a certain concentration range.
33480038	2	67	theme	trap	325:328	arg1	UHPLC-QTRAP-MS/MS					356:372	UHPLC-QTRAP-MS/MS	356:372	UHPLC-QTRAP-MS/MS	356:372	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	2	67	theme	trap	325:328	arg1	spectrometry					342:353	a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry	258:353	a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method	258:380	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	10	68	dep	CONCLUSION	1534:1543	arg1	simple					1623:1628	simple	1623:1628	simple	1623:1628	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	10	68	dep	CONCLUSION	1534:1543	arg1	used					1692:1695	used	1692:1695	can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants	1685:1783	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	10	68	dep	CONCLUSION	1534:1543	arg1	method					1593:1598	The UHPLC-QTRAP-MS/MS pre-column derivatisation method	1545:1598	The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study	1545:1617	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	10	68	dep	CONCLUSION	1534:1543	arg1	accurate					1631:1638	accurate	1631:1638	accurate	1631:1638	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	10	68	dep	CONCLUSION	1534:1543	arg1	sensitive					1645:1653	sensitive	1645:1653	sensitive	1645:1653	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	10	69	with	simple	1623:1628	arg1	repeatability					1666:1678	good repeatability	1661:1678	good repeatability	1661:1678	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	10	70	theme	derivatisation	1578:1591	arg1	simple					1623:1628	simple	1623:1628	simple	1623:1628	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	10	70	theme	derivatisation	1578:1591	arg1	method					1593:1598	The UHPLC-QTRAP-MS/MS pre-column derivatisation method	1545:1598	The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study	1545:1617	CONCLUSION The UHPLC-QTRAP-MS/MS pre-column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.
33480038	2	71	theme	quadrupole	314:323	arg1	UHPLC-QTRAP-MS/MS					356:372	UHPLC-QTRAP-MS/MS	356:372	UHPLC-QTRAP-MS/MS	356:372	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	2	71	theme	quadrupole	314:323	arg1	spectrometry					342:353	a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry	258:353	a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method	258:380	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	2	72	theme	composition	445:455	arg1	determination					413:425	the determination	409:425	the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP)	409:495	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	5	73	dep	analysis	715:722	arg1	analysis					809:816	analysis	809:816	analysis	809:816	Principal component analysis (PCA) and technique for order of preference by similarity to ideal solution (TOPSIS) analysis were used for comprehensive evaluation of the samples.
33480038	2	74	theme	chromatography	299:312	arg1	UHPLC-QTRAP-MS/MS					356:372	UHPLC-QTRAP-MS/MS	356:372	UHPLC-QTRAP-MS/MS	356:372	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	2	74	theme	chromatography	299:312	arg1	spectrometry					342:353	a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry	258:353	a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method	258:380	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	9	75	dep	indexes	1363:1369	arg1	evaluate					1374:1381	evaluate	1374:1381	to evaluate the quality of L. barbarum	1371:1408	RESULTS A rapid quantitative detection method was established, in which the hydrolysis time was reduced from 12 h to 2 h. By using LBP as one of the indexes to evaluate the quality of L. barbarum, L. barbarum from Zhongning County, Ningxia Province, was identified as having the best quality among the varieties tested.
33480038	2	76	theme	monosaccharide	430:443	arg1	composition					445:455	monosaccharide composition	430:455	monosaccharide composition of Lycium barbarum polysaccharide (LBP)	430:495	Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).
33480038	8	77	theme	monosaccharides	1150:1164	arg1	recoveries					1126:1135	Quantitative recoveries	1113:1135	Quantitative recoveries of the seven monosaccharides from the samples	1113:1181	Quantitative recoveries of the seven monosaccharides from the samples ranged from 94.76% to 102.11%.
33480038	8	78	from	recoveries	1126:1135	arg1	samples					1175:1181	the samples	1171:1181	the samples	1171:1181	Quantitative recoveries of the seven monosaccharides from the samples ranged from 94.76% to 102.11%.
33020657	6	0	theme	spectral	1020:1027	arg1	annotation					1029:1038	greatly improved spectral annotation	1003:1038	greatly improved spectral annotation	1003:1038	With greatly improved spectral annotation, coupled with the speed of index-based scoring, MSFragger-Glyco makes it possible to comprehensively interrogate glycoproteomics data and illuminate the many roles of glycosylation.
33020657	1	1	theme	glycopeptides	163:175	arg1	analysis					144:151	mass spectrometric analysis	125:151	mass spectrometric analysis	125:151	Recent advances in methods for enrichment and mass spectrometric analysis of intact glycopeptides have produced large-scale glycoproteomics datasets, but interpreting these data remains challenging.
33020657	1	1	theme	glycopeptides	163:175	arg1	enrichment					110:119	enrichment	110:119	enrichment	110:119	Recent advances in methods for enrichment and mass spectrometric analysis of intact glycopeptides have produced large-scale glycoproteomics datasets, but interpreting these data remains challenging.
33020657	1	2	from	advances	86:93	arg1	methods					98:104	methods	98:104	methods for enrichment and mass spectrometric analysis of intact glycopeptides	98:175	Recent advances in methods for enrichment and mass spectrometric analysis of intact glycopeptides have produced large-scale glycoproteomics datasets, but interpreting these data remains challenging.
33020657	2	3	theme	sensitive	374:382	arg1	identification					384:397	fast and sensitive identification	365:397	fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches	365:455	We present MSFragger-Glyco, a glycoproteomics mode of the MSFragger search engine, for fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches.
33020657	4	4	theme	O-glycoproteomics	618:634	arg1	data					636:639	published O-glycoproteomics data	608:639	published O-glycoproteomics data	608:639	In published O-glycoproteomics data, our method more than doubled the number of glycoPSMs annotated when searching the same glycans as the original search, and yielded 4- to 6-fold increases when expanding searches to include additional glycan compositions and other modifications.
33020657	3	5	theme	matches	559:565	arg1	annotation					514:523	annotation	514:523	annotation of 80% more glycopeptide spectrum matches (glycoPSMs)	514:577	Reanalysis of recent N-glycoproteomics data resulted in annotation of 80% more glycopeptide spectrum matches (glycoPSMs) than previously reported.
33020657	4	6	theme	4-	773:774	arg1	increases					786:794	4- to 6-fold increases	773:794	4- to 6-fold increases	773:794	In published O-glycoproteomics data, our method more than doubled the number of glycoPSMs annotated when searching the same glycans as the original search, and yielded 4- to 6-fold increases when expanding searches to include additional glycan compositions and other modifications.
33020657	3	7	theme	glycopeptide	537:548	arg1	glycoPSMs					568:576	glycoPSMs	568:576	glycoPSMs	568:576	Reanalysis of recent N-glycoproteomics data resulted in annotation of 80% more glycopeptide spectrum matches (glycoPSMs) than previously reported.
33020657	3	7	theme	glycopeptide	537:548	arg1	matches					559:565	80% more glycopeptide spectrum matches	528:565	80% more glycopeptide spectrum matches (glycoPSMs)	528:577	Reanalysis of recent N-glycoproteomics data resulted in annotation of 80% more glycopeptide spectrum matches (glycoPSMs) than previously reported.
33020657	4	8	theme	glycoPSMs	685:693	arg1	number					675:680	the number	671:680	the number of glycoPSMs annotated when searching the same glycans as the original search	671:758	In published O-glycoproteomics data, our method more than doubled the number of glycoPSMs annotated when searching the same glycans as the original search, and yielded 4- to 6-fold increases when expanding searches to include additional glycan compositions and other modifications.
33020657	2	9	gly	glycopeptides	418:430	arg2	glycopeptides					418:430	O-linked glycopeptides	409:430	O-linked glycopeptides	409:430	We present MSFragger-Glyco, a glycoproteomics mode of the MSFragger search engine, for fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches.
33020657	5	10	theme	Expanded	887:894	arg1	searches					896:903	Expanded searches	887:903	Expanded searches	887:903	Expanded searches also revealed many sulfated and complex glycans that remained hidden to the original search.
33020657	1	11	theme	Recent	79:84	arg1	advances					86:93	Recent advances	79:93	Recent advances in methods for enrichment and mass spectrometric analysis of intact glycopeptides	79:175	Recent advances in methods for enrichment and mass spectrometric analysis of intact glycopeptides have produced large-scale glycoproteomics datasets, but interpreting these data remains challenging.
33020657	2	12	theme	O-linked	409:416	arg1	glycopeptides					418:430	O-linked glycopeptides	409:430	O-linked glycopeptides	409:430	We present MSFragger-Glyco, a glycoproteomics mode of the MSFragger search engine, for fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches.
33020657	3	13	theme	recent	472:477	arg1	data					497:500	recent N-glycoproteomics data	472:500	recent N-glycoproteomics data	472:500	Reanalysis of recent N-glycoproteomics data resulted in annotation of 80% more glycopeptide spectrum matches (glycoPSMs) than previously reported.
33020657	3	14	gly	glycopeptide	537:548	arg2	glycopeptide					537:548	80% more glycopeptide spectrum matches	528:565	80% more glycopeptide spectrum matches (glycoPSMs)	528:577	Reanalysis of recent N-glycoproteomics data resulted in annotation of 80% more glycopeptide spectrum matches (glycoPSMs) than previously reported.
33020657	1	15	theme	large-scale	191:201	arg1	datasets					219:226	large-scale glycoproteomics datasets	191:226	large-scale glycoproteomics datasets	191:226	Recent advances in methods for enrichment and mass spectrometric analysis of intact glycopeptides have produced large-scale glycoproteomics datasets, but interpreting these data remains challenging.
33020657	6	16	theme	improved	1011:1018	arg1	annotation					1029:1038	greatly improved spectral annotation	1003:1038	greatly improved spectral annotation	1003:1038	With greatly improved spectral annotation, coupled with the speed of index-based scoring, MSFragger-Glyco makes it possible to comprehensively interrogate glycoproteomics data and illuminate the many roles of glycosylation.
33020657	2	17	theme	engine	353:358	arg1	mode					324:327	a glycoproteomics mode	306:327	a glycoproteomics mode of the MSFragger search engine	306:358	We present MSFragger-Glyco, a glycoproteomics mode of the MSFragger search engine, for fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches.
33020657	2	17	theme	engine	353:358	arg1	MSFragger-Glyco					289:303	MSFragger-Glyco	289:303	MSFragger-Glyco	289:303	We present MSFragger-Glyco, a glycoproteomics mode of the MSFragger search engine, for fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches.
33020657	1	18	theme	glycoproteomics	203:217	arg1	datasets					219:226	large-scale glycoproteomics datasets	191:226	large-scale glycoproteomics datasets	191:226	Recent advances in methods for enrichment and mass spectrometric analysis of intact glycopeptides have produced large-scale glycoproteomics datasets, but interpreting these data remains challenging.
33020657	0	19	theme	Fast	0:3	arg1	N-					23:24	N-	23:24	N-	23:24	Fast and comprehensive N- and O-glycoproteomics analysis with MSFragger-Glyco.
33020657	4	20	dep	4-	773:774	arg1	to					776:777	to	776:777	to	776:777	In published O-glycoproteomics data, our method more than doubled the number of glycoPSMs annotated when searching the same glycans as the original search, and yielded 4- to 6-fold increases when expanding searches to include additional glycan compositions and other modifications.
33020657	2	21	theme	search	346:351	arg1	engine					353:358	the MSFragger search engine	332:358	the MSFragger search engine	332:358	We present MSFragger-Glyco, a glycoproteomics mode of the MSFragger search engine, for fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches.
33020657	5	22	theme	original	981:988	arg1	search					990:995	the original search	977:995	the original search	977:995	Expanded searches also revealed many sulfated and complex glycans that remained hidden to the original search.
33020657	2	23	theme	MSFragger	336:344	arg1	engine					353:358	the MSFragger search engine	332:358	the MSFragger search engine	332:358	We present MSFragger-Glyco, a glycoproteomics mode of the MSFragger search engine, for fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches.
33020657	0	24	theme	comprehensive	9:21	arg1	N-					23:24	N-	23:24	N-	23:24	Fast and comprehensive N- and O-glycoproteomics analysis with MSFragger-Glyco.
33020657	4	25	theme	published	608:616	arg1	data					636:639	published O-glycoproteomics data	608:639	published O-glycoproteomics data	608:639	In published O-glycoproteomics data, our method more than doubled the number of glycoPSMs annotated when searching the same glycans as the original search, and yielded 4- to 6-fold increases when expanding searches to include additional glycan compositions and other modifications.
33020657	2	26	theme	glycoproteomics	308:322	arg1	mode					324:327	a glycoproteomics mode	306:327	a glycoproteomics mode of the MSFragger search engine	306:358	We present MSFragger-Glyco, a glycoproteomics mode of the MSFragger search engine, for fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches.
33020657	2	26	theme	glycoproteomics	308:322	arg1	MSFragger-Glyco					289:303	MSFragger-Glyco	289:303	MSFragger-Glyco	289:303	We present MSFragger-Glyco, a glycoproteomics mode of the MSFragger search engine, for fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches.
33020657	2	27	link	O-linked	409:416	arg1	glycopeptides					418:430	O-linked glycopeptides	409:430	O-linked glycopeptides	409:430	We present MSFragger-Glyco, a glycoproteomics mode of the MSFragger search engine, for fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches.
33020657	3	28	theme	spectrum	550:557	arg1	glycoPSMs					568:576	glycoPSMs	568:576	glycoPSMs	568:576	Reanalysis of recent N-glycoproteomics data resulted in annotation of 80% more glycopeptide spectrum matches (glycoPSMs) than previously reported.
33020657	3	28	theme	spectrum	550:557	arg1	matches					559:565	80% more glycopeptide spectrum matches	528:565	80% more glycopeptide spectrum matches (glycoPSMs)	528:577	Reanalysis of recent N-glycoproteomics data resulted in annotation of 80% more glycopeptide spectrum matches (glycoPSMs) than previously reported.
33020657	2	29	theme	searches	448:455	arg1	identification					384:397	fast and sensitive identification	365:397	fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches	365:455	We present MSFragger-Glyco, a glycoproteomics mode of the MSFragger search engine, for fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches.
33020657	2	30	theme	fast	365:368	arg1	identification					384:397	fast and sensitive identification	365:397	fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches	365:455	We present MSFragger-Glyco, a glycoproteomics mode of the MSFragger search engine, for fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches.
33020657	3	31	theme	data	497:500	arg1	Reanalysis					458:467	Reanalysis	458:467	Reanalysis of recent N-glycoproteomics data	458:500	Reanalysis of recent N-glycoproteomics data resulted in annotation of 80% more glycopeptide spectrum matches (glycoPSMs) than previously reported.
33020657	2	32	theme	glycan	441:446	arg1	searches					448:455	open glycan searches	436:455	open glycan searches	436:455	We present MSFragger-Glyco, a glycoproteomics mode of the MSFragger search engine, for fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches.
33020657	6	33	theme	glycoproteomics	1153:1167	arg1	data					1169:1172	glycoproteomics data	1153:1172	glycoproteomics data	1153:1172	With greatly improved spectral annotation, coupled with the speed of index-based scoring, MSFragger-Glyco makes it possible to comprehensively interrogate glycoproteomics data and illuminate the many roles of glycosylation.
33020657	5	34	theme	many	919:922	arg1	glycans					945:951	many sulfated and complex glycans	919:951	many sulfated and complex glycans that remained hidden to the original search	919:995	Expanded searches also revealed many sulfated and complex glycans that remained hidden to the original search.
33020657	5	35	theme	sulfated	924:931	arg1	glycans					945:951	many sulfated and complex glycans	919:951	many sulfated and complex glycans that remained hidden to the original search	919:995	Expanded searches also revealed many sulfated and complex glycans that remained hidden to the original search.
33020657	2	36	theme	open	436:439	arg1	searches					448:455	open glycan searches	436:455	open glycan searches	436:455	We present MSFragger-Glyco, a glycoproteomics mode of the MSFragger search engine, for fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches.
33020657	5	37	theme	complex	937:943	arg1	glycans					945:951	many sulfated and complex glycans	919:951	many sulfated and complex glycans that remained hidden to the original search	919:995	Expanded searches also revealed many sulfated and complex glycans that remained hidden to the original search.
33020657	6	38	theme	glycosylation	1207:1219	arg1	roles					1198:1202	the many roles	1189:1202	the many roles of glycosylation	1189:1219	With greatly improved spectral annotation, coupled with the speed of index-based scoring, MSFragger-Glyco makes it possible to comprehensively interrogate glycoproteomics data and illuminate the many roles of glycosylation.
33020657	4	39	theme	other	866:870	arg1	modifications					872:884	other modifications	866:884	other modifications	866:884	In published O-glycoproteomics data, our method more than doubled the number of glycoPSMs annotated when searching the same glycans as the original search, and yielded 4- to 6-fold increases when expanding searches to include additional glycan compositions and other modifications.
33020657	1	40	theme	mass	125:128	arg1	analysis					144:151	mass spectrometric analysis	125:151	mass spectrometric analysis	125:151	Recent advances in methods for enrichment and mass spectrometric analysis of intact glycopeptides have produced large-scale glycoproteomics datasets, but interpreting these data remains challenging.
33020657	6	41	theme	scoring	1079:1085	arg1	speed					1058:1062	the speed	1054:1062	the speed of index-based scoring	1054:1085	With greatly improved spectral annotation, coupled with the speed of index-based scoring, MSFragger-Glyco makes it possible to comprehensively interrogate glycoproteomics data and illuminate the many roles of glycosylation.
33020657	1	42	theme	spectrometric	130:142	arg1	analysis					144:151	mass spectrometric analysis	125:151	mass spectrometric analysis	125:151	Recent advances in methods for enrichment and mass spectrometric analysis of intact glycopeptides have produced large-scale glycoproteomics datasets, but interpreting these data remains challenging.
33020657	6	43	theme	many	1193:1196	arg1	roles					1198:1202	the many roles	1189:1202	the many roles of glycosylation	1189:1219	With greatly improved spectral annotation, coupled with the speed of index-based scoring, MSFragger-Glyco makes it possible to comprehensively interrogate glycoproteomics data and illuminate the many roles of glycosylation.
33020657	1	44	gly	glycopeptides	163:175	arg2	glycopeptides					163:175	intact glycopeptides	156:175	intact glycopeptides	156:175	Recent advances in methods for enrichment and mass spectrometric analysis of intact glycopeptides have produced large-scale glycoproteomics datasets, but interpreting these data remains challenging.
33020657	4	45	theme	same	724:727	arg1	search					753:758	the original search	740:758	the original search	740:758	In published O-glycoproteomics data, our method more than doubled the number of glycoPSMs annotated when searching the same glycans as the original search, and yielded 4- to 6-fold increases when expanding searches to include additional glycan compositions and other modifications.
33020657	4	45	theme	same	724:727	arg1	glycans					729:735	the same glycans	720:735	the same glycans	720:735	In published O-glycoproteomics data, our method more than doubled the number of glycoPSMs annotated when searching the same glycans as the original search, and yielded 4- to 6-fold increases when expanding searches to include additional glycan compositions and other modifications.
33020657	3	46	theme	N-glycoproteomics	479:495	arg1	data					497:500	recent N-glycoproteomics data	472:500	recent N-glycoproteomics data	472:500	Reanalysis of recent N-glycoproteomics data resulted in annotation of 80% more glycopeptide spectrum matches (glycoPSMs) than previously reported.
33020657	4	47	theme	glycan	842:847	arg1	compositions					849:860	additional glycan compositions	831:860	additional glycan compositions	831:860	In published O-glycoproteomics data, our method more than doubled the number of glycoPSMs annotated when searching the same glycans as the original search, and yielded 4- to 6-fold increases when expanding searches to include additional glycan compositions and other modifications.
33020657	0	48	with	analysis	48:55	arg1	MSFragger-Glyco					62:76	MSFragger-Glyco	62:76	MSFragger-Glyco	62:76	Fast and comprehensive N- and O-glycoproteomics analysis with MSFragger-Glyco.
33020657	2	49	theme	N-	402:403	arg1	identification					384:397	fast and sensitive identification	365:397	fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches	365:455	We present MSFragger-Glyco, a glycoproteomics mode of the MSFragger search engine, for fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches.
33020657	2	50	theme	glycopeptides	418:430	arg1	identification					384:397	fast and sensitive identification	365:397	fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches	365:455	We present MSFragger-Glyco, a glycoproteomics mode of the MSFragger search engine, for fast and sensitive identification of N- and O-linked glycopeptides and open glycan searches.
33020657	6	51	theme	index-based	1067:1077	arg1	scoring					1079:1085	index-based scoring	1067:1085	index-based scoring	1067:1085	With greatly improved spectral annotation, coupled with the speed of index-based scoring, MSFragger-Glyco makes it possible to comprehensively interrogate glycoproteomics data and illuminate the many roles of glycosylation.
33020657	4	52	theme	additional	831:840	arg1	compositions					849:860	additional glycan compositions	831:860	additional glycan compositions	831:860	In published O-glycoproteomics data, our method more than doubled the number of glycoPSMs annotated when searching the same glycans as the original search, and yielded 4- to 6-fold increases when expanding searches to include additional glycan compositions and other modifications.
33020657	1	53	theme	intact	156:161	arg1	glycopeptides					163:175	intact glycopeptides	156:175	intact glycopeptides	156:175	Recent advances in methods for enrichment and mass spectrometric analysis of intact glycopeptides have produced large-scale glycoproteomics datasets, but interpreting these data remains challenging.
33020657	3	54	theme	more	532:535	arg1	glycoPSMs					568:576	glycoPSMs	568:576	glycoPSMs	568:576	Reanalysis of recent N-glycoproteomics data resulted in annotation of 80% more glycopeptide spectrum matches (glycoPSMs) than previously reported.
33020657	3	54	theme	more	532:535	arg1	matches					559:565	80% more glycopeptide spectrum matches	528:565	80% more glycopeptide spectrum matches (glycoPSMs)	528:577	Reanalysis of recent N-glycoproteomics data resulted in annotation of 80% more glycopeptide spectrum matches (glycoPSMs) than previously reported.
33020657	4	55	theme	original	744:751	arg1	search					753:758	the original search	740:758	the original search	740:758	In published O-glycoproteomics data, our method more than doubled the number of glycoPSMs annotated when searching the same glycans as the original search, and yielded 4- to 6-fold increases when expanding searches to include additional glycan compositions and other modifications.
33020657	4	55	theme	original	744:751	arg1	glycans					729:735	the same glycans	720:735	the same glycans	720:735	In published O-glycoproteomics data, our method more than doubled the number of glycoPSMs annotated when searching the same glycans as the original search, and yielded 4- to 6-fold increases when expanding searches to include additional glycan compositions and other modifications.
34605629	4	0	theme	iridescent	762:771	arg1	films					773:777	the iridescent films	758:777	the iridescent films	758:777	Simultaneously, the iridescent films showed a conspicuous redshift and enlarged initial pitch without obstruction of the chiral structure.
34605629	5	1	dep	humidity	910:917	arg1	response					884:891	response	884:891	response	884:891	In response to environmental humidity, the structural colors of the iridescent composite films can be changed by regulating their chiral nematic structure.
34605629	1	2	theme	environmental	180:192	arg1	functions					213:221	environmental stimuli-responsive functions	180:221	environmental stimuli-responsive functions from entirely biobased materials	180:254	The fabrication of biomimetic photonic materials with environmental stimuli-responsive functions from entirely biobased materials is becoming increasingly challenging with the growing demand for biodegradable materials.
34605629	2	3	from	effect	358:363	arg1	color					461:465	tunable structural color	442:465	tunable structural color	442:465	Herein, the effect of glucan with different molecular weights on the mechanical performance and tunable structural color of iridescent CNC composite films was investigated.
34605629	2	3	from	effect	358:363	arg1	performance					426:436	mechanical performance	415:436	mechanical performance	415:436	Herein, the effect of glucan with different molecular weights on the mechanical performance and tunable structural color of iridescent CNC composite films was investigated.
34605629	6	4	theme	color	1098:1102	arg1	change					1104:1109	a reversible structural color change	1074:1109	a reversible structural color change between blue and red at RH between 50 and 98%	1074:1155	In particular, the films demonstrate a reversible structural color change between blue and red at RH between 50 and 98%.
34605629	1	5	theme	stimuli-responsive	194:211	arg1	functions					213:221	environmental stimuli-responsive functions	180:221	environmental stimuli-responsive functions from entirely biobased materials	180:254	The fabrication of biomimetic photonic materials with environmental stimuli-responsive functions from entirely biobased materials is becoming increasingly challenging with the growing demand for biodegradable materials.
34605629	7	6	contain	have	1208:1211	arg1	films					1202:1206	The resulting biobased iridescent composite films	1158:1206	The resulting biobased iridescent composite films	1158:1206	The resulting biobased iridescent composite films have potential applications in decorative coating, optical and humidity sensing, and anticounterfeiting.
34605629	7	6	contain	have	1208:1211	arg2	applications					1223:1234	potential applications	1213:1234	potential applications	1213:1234	The resulting biobased iridescent composite films have potential applications in decorative coating, optical and humidity sensing, and anticounterfeiting.
34605629	6	7	theme	structural	1087:1096	arg1	change					1104:1109	a reversible structural color change	1074:1109	a reversible structural color change between blue and red at RH between 50 and 98%	1074:1155	In particular, the films demonstrate a reversible structural color change between blue and red at RH between 50 and 98%.
34605629	7	8	theme	iridescent	1181:1190	arg1	films					1202:1206	The resulting biobased iridescent composite films	1158:1206	The resulting biobased iridescent composite films	1158:1206	The resulting biobased iridescent composite films have potential applications in decorative coating, optical and humidity sensing, and anticounterfeiting.
34605629	3	9	theme	CNCs	594:597	arg1	performance					579:589	the self-assembly performance	561:589	the self-assembly performance of CNCs	561:597	The existence of glucan did not influence the self-assembly performance of CNCs, but rather led to an improvement in the mechanical performance, enabling cholesteric CNC composite films with an adjustable structural color.
34605629	1	10	theme	biodegradable	321:333	arg1	materials					335:343	biodegradable materials	321:343	biodegradable materials	321:343	The fabrication of biomimetic photonic materials with environmental stimuli-responsive functions from entirely biobased materials is becoming increasingly challenging with the growing demand for biodegradable materials.
34605629	0	11	theme	Reinforced	104:113	arg1	Mechanics					115:123	Reinforced Mechanics	104:123	Reinforced Mechanics	104:123	Chiral Cellulose Nanocrystal Humidity-Responsive Iridescent Films with Glucan for Tuned Iridescence and Reinforced Mechanics.
34605629	7	12	theme	composite	1192:1200	arg1	films					1202:1206	The resulting biobased iridescent composite films	1158:1206	The resulting biobased iridescent composite films	1158:1206	The resulting biobased iridescent composite films have potential applications in decorative coating, optical and humidity sensing, and anticounterfeiting.
34605629	2	13	theme	mechanical	415:424	arg1	performance					426:436	mechanical performance	415:436	mechanical performance	415:436	Herein, the effect of glucan with different molecular weights on the mechanical performance and tunable structural color of iridescent CNC composite films was investigated.
34605629	2	14	theme	different	380:388	arg1	weights					400:406	different molecular weights	380:406	different molecular weights	380:406	Herein, the effect of glucan with different molecular weights on the mechanical performance and tunable structural color of iridescent CNC composite films was investigated.
34605629	7	15	theme	humidity	1271:1278	arg1	sensing					1280:1286	optical and humidity sensing	1259:1286	optical and humidity sensing	1259:1286	The resulting biobased iridescent composite films have potential applications in decorative coating, optical and humidity sensing, and anticounterfeiting.
34605629	7	16	theme	resulting	1162:1170	arg1	films					1202:1206	The resulting biobased iridescent composite films	1158:1206	The resulting biobased iridescent composite films	1158:1206	The resulting biobased iridescent composite films have potential applications in decorative coating, optical and humidity sensing, and anticounterfeiting.
34605629	5	17	theme	structural	924:933	arg1	colors					935:940	the structural colors	920:940	the structural colors of the iridescent composite films	920:974	In response to environmental humidity, the structural colors of the iridescent composite films can be changed by regulating their chiral nematic structure.
34605629	4	18	theme	chiral	863:868	arg1	structure					870:878	the chiral structure	859:878	the chiral structure	859:878	Simultaneously, the iridescent films showed a conspicuous redshift and enlarged initial pitch without obstruction of the chiral structure.
34605629	5	19	theme	chiral	1011:1016	arg1	structure					1026:1034	their chiral nematic structure	1005:1034	their chiral nematic structure	1005:1034	In response to environmental humidity, the structural colors of the iridescent composite films can be changed by regulating their chiral nematic structure.
34605629	2	20	with	glucan	368:373	arg1	weights					400:406	different molecular weights	380:406	different molecular weights	380:406	Herein, the effect of glucan with different molecular weights on the mechanical performance and tunable structural color of iridescent CNC composite films was investigated.
34605629	7	21	theme	biobased	1172:1179	arg1	films					1202:1206	The resulting biobased iridescent composite films	1158:1206	The resulting biobased iridescent composite films	1158:1206	The resulting biobased iridescent composite films have potential applications in decorative coating, optical and humidity sensing, and anticounterfeiting.
34605629	2	22	dep	performance	426:436	arg1	the					411:413	the	411:413	the	411:413	Herein, the effect of glucan with different molecular weights on the mechanical performance and tunable structural color of iridescent CNC composite films was investigated.
34605629	5	23	theme	iridescent	949:958	arg1	films					970:974	the iridescent composite films	945:974	the iridescent composite films	945:974	In response to environmental humidity, the structural colors of the iridescent composite films can be changed by regulating their chiral nematic structure.
34605629	0	24	theme	Cellulose	7:15	arg1	Films					60:64	Chiral Cellulose Nanocrystal Humidity-Responsive Iridescent Films	0:64	Chiral Cellulose Nanocrystal Humidity-Responsive Iridescent Films with Glucan for Tuned Iridescence and Reinforced Mechanics.	0:124	Chiral Cellulose Nanocrystal Humidity-Responsive Iridescent Films with Glucan for Tuned Iridescence and Reinforced Mechanics.
34605629	1	25	with	fabrication	130:140	arg1	functions					213:221	environmental stimuli-responsive functions	180:221	environmental stimuli-responsive functions from entirely biobased materials	180:254	The fabrication of biomimetic photonic materials with environmental stimuli-responsive functions from entirely biobased materials is becoming increasingly challenging with the growing demand for biodegradable materials.
34605629	4	26	theme	initial	822:828	arg1	pitch					830:834	initial pitch	822:834	initial pitch without obstruction of the chiral structure	822:878	Simultaneously, the iridescent films showed a conspicuous redshift and enlarged initial pitch without obstruction of the chiral structure.
34605629	2	27	theme	structural	450:459	arg1	color					461:465	tunable structural color	442:465	tunable structural color	442:465	Herein, the effect of glucan with different molecular weights on the mechanical performance and tunable structural color of iridescent CNC composite films was investigated.
34605629	0	28	theme	Chiral	0:5	arg1	Films					60:64	Chiral Cellulose Nanocrystal Humidity-Responsive Iridescent Films	0:64	Chiral Cellulose Nanocrystal Humidity-Responsive Iridescent Films with Glucan for Tuned Iridescence and Reinforced Mechanics.	0:124	Chiral Cellulose Nanocrystal Humidity-Responsive Iridescent Films with Glucan for Tuned Iridescence and Reinforced Mechanics.
34605629	7	29	theme	decorative	1239:1248	arg1	coating					1250:1256	decorative coating	1239:1256	decorative coating	1239:1256	The resulting biobased iridescent composite films have potential applications in decorative coating, optical and humidity sensing, and anticounterfeiting.
34605629	2	30	theme	glucan	368:373	arg1	effect					358:363	the effect	354:363	the effect of glucan with different molecular weights on the mechanical performance and tunable structural color of iridescent CNC composite films	354:499	Herein, the effect of glucan with different molecular weights on the mechanical performance and tunable structural color of iridescent CNC composite films was investigated.
34605629	1	31	theme	biobased	237:244	arg1	materials					246:254	entirely biobased materials	228:254	entirely biobased materials	228:254	The fabrication of biomimetic photonic materials with environmental stimuli-responsive functions from entirely biobased materials is becoming increasingly challenging with the growing demand for biodegradable materials.
34605629	0	32	theme	Humidity-Responsive	29:47	arg1	Films					60:64	Chiral Cellulose Nanocrystal Humidity-Responsive Iridescent Films	0:64	Chiral Cellulose Nanocrystal Humidity-Responsive Iridescent Films with Glucan for Tuned Iridescence and Reinforced Mechanics.	0:124	Chiral Cellulose Nanocrystal Humidity-Responsive Iridescent Films with Glucan for Tuned Iridescence and Reinforced Mechanics.
34605629	5	33	theme	environmental	896:908	arg1	humidity					910:917	environmental humidity	896:917	environmental humidity	896:917	In response to environmental humidity, the structural colors of the iridescent composite films can be changed by regulating their chiral nematic structure.
34605629	5	34	theme	nematic	1018:1024	arg1	structure					1026:1034	their chiral nematic structure	1005:1034	their chiral nematic structure	1005:1034	In response to environmental humidity, the structural colors of the iridescent composite films can be changed by regulating their chiral nematic structure.
34605629	2	35	theme	films	495:499	arg1	color					461:465	tunable structural color	442:465	tunable structural color	442:465	Herein, the effect of glucan with different molecular weights on the mechanical performance and tunable structural color of iridescent CNC composite films was investigated.
34605629	2	35	theme	films	495:499	arg1	performance					426:436	mechanical performance	415:436	mechanical performance	415:436	Herein, the effect of glucan with different molecular weights on the mechanical performance and tunable structural color of iridescent CNC composite films was investigated.
34605629	0	36	theme	Nanocrystal	17:27	arg1	Films					60:64	Chiral Cellulose Nanocrystal Humidity-Responsive Iridescent Films	0:64	Chiral Cellulose Nanocrystal Humidity-Responsive Iridescent Films with Glucan for Tuned Iridescence and Reinforced Mechanics.	0:124	Chiral Cellulose Nanocrystal Humidity-Responsive Iridescent Films with Glucan for Tuned Iridescence and Reinforced Mechanics.
34605629	3	37	theme	adjustable	713:722	arg1	color					735:739	an adjustable structural color	710:739	an adjustable structural color	710:739	The existence of glucan did not influence the self-assembly performance of CNCs, but rather led to an improvement in the mechanical performance, enabling cholesteric CNC composite films with an adjustable structural color.
34605629	2	38	theme	composite	485:493	arg1	films					495:499	iridescent CNC composite films	470:499	iridescent CNC composite films	470:499	Herein, the effect of glucan with different molecular weights on the mechanical performance and tunable structural color of iridescent CNC composite films was investigated.
34605629	3	39	theme	structural	724:733	arg1	color					735:739	an adjustable structural color	710:739	an adjustable structural color	710:739	The existence of glucan did not influence the self-assembly performance of CNCs, but rather led to an improvement in the mechanical performance, enabling cholesteric CNC composite films with an adjustable structural color.
34605629	2	40	theme	tunable	442:448	arg1	color					461:465	tunable structural color	442:465	tunable structural color	442:465	Herein, the effect of glucan with different molecular weights on the mechanical performance and tunable structural color of iridescent CNC composite films was investigated.
34605629	2	41	theme	CNC	481:483	arg1	films					495:499	iridescent CNC composite films	470:499	iridescent CNC composite films	470:499	Herein, the effect of glucan with different molecular weights on the mechanical performance and tunable structural color of iridescent CNC composite films was investigated.
34605629	0	42	theme	Iridescent	49:58	arg1	Films					60:64	Chiral Cellulose Nanocrystal Humidity-Responsive Iridescent Films	0:64	Chiral Cellulose Nanocrystal Humidity-Responsive Iridescent Films with Glucan for Tuned Iridescence and Reinforced Mechanics.	0:124	Chiral Cellulose Nanocrystal Humidity-Responsive Iridescent Films with Glucan for Tuned Iridescence and Reinforced Mechanics.
34605629	5	43	theme	composite	960:968	arg1	films					970:974	the iridescent composite films	945:974	the iridescent composite films	945:974	In response to environmental humidity, the structural colors of the iridescent composite films can be changed by regulating their chiral nematic structure.
34605629	2	44	theme	iridescent	470:479	arg1	films					495:499	iridescent CNC composite films	470:499	iridescent CNC composite films	470:499	Herein, the effect of glucan with different molecular weights on the mechanical performance and tunable structural color of iridescent CNC composite films was investigated.
34605629	7	45	theme	potential	1213:1221	arg1	applications					1223:1234	potential applications	1213:1234	potential applications	1213:1234	The resulting biobased iridescent composite films have potential applications in decorative coating, optical and humidity sensing, and anticounterfeiting.
34605629	3	46	theme	mechanical	640:649	arg1	performance					651:661	the mechanical performance	636:661	the mechanical performance	636:661	The existence of glucan did not influence the self-assembly performance of CNCs, but rather led to an improvement in the mechanical performance, enabling cholesteric CNC composite films with an adjustable structural color.
34605629	4	47	theme	conspicuous	788:798	arg1	redshift					800:807	a conspicuous redshift	786:807	a conspicuous redshift	786:807	Simultaneously, the iridescent films showed a conspicuous redshift and enlarged initial pitch without obstruction of the chiral structure.
34605629	1	48	from	materials	246:254	arg1	functions					213:221	environmental stimuli-responsive functions	180:221	environmental stimuli-responsive functions from entirely biobased materials	180:254	The fabrication of biomimetic photonic materials with environmental stimuli-responsive functions from entirely biobased materials is becoming increasingly challenging with the growing demand for biodegradable materials.
34605629	3	49	theme	self-assembly	565:577	arg1	performance					579:589	the self-assembly performance	561:589	the self-assembly performance of CNCs	561:597	The existence of glucan did not influence the self-assembly performance of CNCs, but rather led to an improvement in the mechanical performance, enabling cholesteric CNC composite films with an adjustable structural color.
34605629	6	50	from	RH	1135:1136	arg1	blue					1119:1122	blue	1119:1122	blue	1119:1122	In particular, the films demonstrate a reversible structural color change between blue and red at RH between 50 and 98%.
34605629	3	51	theme	composite	689:697	arg1	films					699:703	cholesteric CNC composite films	673:703	cholesteric CNC composite films	673:703	The existence of glucan did not influence the self-assembly performance of CNCs, but rather led to an improvement in the mechanical performance, enabling cholesteric CNC composite films with an adjustable structural color.
34605629	1	52	dep	becoming	259:266	arg1	challenging					281:291	challenging	281:291	is becoming increasingly challenging with the growing demand for biodegradable materials	256:343	The fabrication of biomimetic photonic materials with environmental stimuli-responsive functions from entirely biobased materials is becoming increasingly challenging with the growing demand for biodegradable materials.
34605629	1	53	theme	biomimetic	145:154	arg1	materials					165:173	biomimetic photonic materials	145:173	biomimetic photonic materials	145:173	The fabrication of biomimetic photonic materials with environmental stimuli-responsive functions from entirely biobased materials is becoming increasingly challenging with the growing demand for biodegradable materials.
34605629	3	54	from	improvement	621:631	arg1	performance					651:661	the mechanical performance	636:661	the mechanical performance	636:661	The existence of glucan did not influence the self-assembly performance of CNCs, but rather led to an improvement in the mechanical performance, enabling cholesteric CNC composite films with an adjustable structural color.
34605629	4	55	theme	structure	870:878	arg1	obstruction					844:854	obstruction	844:854	obstruction of the chiral structure	844:878	Simultaneously, the iridescent films showed a conspicuous redshift and enlarged initial pitch without obstruction of the chiral structure.
34605629	1	56	theme	photonic	156:163	arg1	materials					165:173	biomimetic photonic materials	145:173	biomimetic photonic materials	145:173	The fabrication of biomimetic photonic materials with environmental stimuli-responsive functions from entirely biobased materials is becoming increasingly challenging with the growing demand for biodegradable materials.
34605629	2	57	theme	molecular	390:398	arg1	weights					400:406	different molecular weights	380:406	different molecular weights	380:406	Herein, the effect of glucan with different molecular weights on the mechanical performance and tunable structural color of iridescent CNC composite films was investigated.
34605629	0	58	theme	Tuned	82:86	arg1	Iridescence					88:98	Tuned Iridescence	82:98	Tuned Iridescence	82:98	Chiral Cellulose Nanocrystal Humidity-Responsive Iridescent Films with Glucan for Tuned Iridescence and Reinforced Mechanics.
34605629	3	59	theme	cholesteric	673:683	arg1	films					699:703	cholesteric CNC composite films	673:703	cholesteric CNC composite films	673:703	The existence of glucan did not influence the self-assembly performance of CNCs, but rather led to an improvement in the mechanical performance, enabling cholesteric CNC composite films with an adjustable structural color.
34605629	3	60	theme	glucan	536:541	arg1	existence					523:531	The existence	519:531	The existence of glucan	519:541	The existence of glucan did not influence the self-assembly performance of CNCs, but rather led to an improvement in the mechanical performance, enabling cholesteric CNC composite films with an adjustable structural color.
34605629	1	61	theme	materials	165:173	arg1	fabrication					130:140	The fabrication	126:140	The fabrication of biomimetic photonic materials with environmental stimuli-responsive functions from entirely biobased materials	126:254	The fabrication of biomimetic photonic materials with environmental stimuli-responsive functions from entirely biobased materials is becoming increasingly challenging with the growing demand for biodegradable materials.
34605629	5	62	theme	films	970:974	arg1	colors					935:940	the structural colors	920:940	the structural colors of the iridescent composite films	920:974	In response to environmental humidity, the structural colors of the iridescent composite films can be changed by regulating their chiral nematic structure.
34605629	6	63	theme	reversible	1076:1085	arg1	change					1104:1109	a reversible structural color change	1074:1109	a reversible structural color change between blue and red at RH between 50 and 98%	1074:1155	In particular, the films demonstrate a reversible structural color change between blue and red at RH between 50 and 98%.
34605629	3	64	theme	CNC	685:687	arg1	films					699:703	cholesteric CNC composite films	673:703	cholesteric CNC composite films	673:703	The existence of glucan did not influence the self-assembly performance of CNCs, but rather led to an improvement in the mechanical performance, enabling cholesteric CNC composite films with an adjustable structural color.
34605629	0	65	with	Films	60:64	arg1	Glucan					71:76	Glucan	71:76	Glucan	71:76	Chiral Cellulose Nanocrystal Humidity-Responsive Iridescent Films with Glucan for Tuned Iridescence and Reinforced Mechanics.
34605629	7	66	theme	optical	1259:1265	arg1	sensing					1280:1286	optical and humidity sensing	1259:1286	optical and humidity sensing	1259:1286	The resulting biobased iridescent composite films have potential applications in decorative coating, optical and humidity sensing, and anticounterfeiting.
34605629	1	67	theme	growing	302:308	arg1	demand					310:315	the growing demand	298:315	the growing demand for biodegradable materials	298:343	The fabrication of biomimetic photonic materials with environmental stimuli-responsive functions from entirely biobased materials is becoming increasingly challenging with the growing demand for biodegradable materials.
33900081	3	0	theme	comprehensive	657:669	arg1	profiling					671:679	a comprehensive profiling	655:679	a comprehensive profiling of native ganglioside mixtures extracted and purified from GBM specimens	655:752	Since gangliosides (GGs) are acknowledged as tumor-associated antigens, we have carried out here a comprehensive profiling of native ganglioside mixtures extracted and purified from GBM specimens.
33900081	1	1	theme	treatment	362:370	arg1	options					372:378	the limited treatment options	350:378	the limited treatment options available	350:388	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	8	2	theme	fragment	1638:1645	arg1	ions					1647:1650	the diagnostic fragment ions	1623:1650	the diagnostic fragment ions	1623:1650	By the occurrence of only one mobility feature and the diagnostic fragment ions, the IMS tandem MS conducted using collision-induced dissociation (CID) disclosed for the first time the presence of GT1c(d18:1/24:1) newly proposed here as a potential GBM marker.
33900081	5	3	theme	components	1182:1191	arg1	identification					1138:1151	the identification	1134:1151	the identification of no less than 160 distinct components, which represents 3-fold the number of structures identified before	1134:1259	GG separation by IMS according to the charge state, carbohydrate chain length, degree of sialylation, and ceramide composition led to the identification of no less than 160 distinct components, which represents 3-fold the number of structures identified before.
33900081	4	4	theme	IMS	833:835	arg1	MS					837:838	IMS MS	833:838	IMS MS	833:838	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	4	4	theme	IMS	833:835	arg1	spectrometry					819:830	high performance ion mobility separation mass spectrometry	773:830	high performance ion mobility separation mass spectrometry (IMS MS)	773:839	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	3	5	theme	ganglioside	691:701	arg1	mixtures					703:710	native ganglioside mixtures	684:710	native ganglioside mixtures extracted and purified from GBM specimens	684:752	Since gangliosides (GGs) are acknowledged as tumor-associated antigens, we have carried out here a comprehensive profiling of native ganglioside mixtures extracted and purified from GBM specimens.
33900081	8	6	theme	IMS	1657:1659	arg1	MS					1668:1669	the IMS tandem MS	1653:1669	the IMS tandem MS conducted using collision-induced dissociation (CID)	1653:1722	By the occurrence of only one mobility feature and the diagnostic fragment ions, the IMS tandem MS conducted using collision-induced dissociation (CID) disclosed for the first time the presence of GT1c(d18:1/24:1) newly proposed here as a potential GBM marker.
33900081	3	7	theme	GBM	740:742	arg1	specimens					744:752	GBM specimens	740:752	GBM specimens	740:752	Since gangliosides (GGs) are acknowledged as tumor-associated antigens, we have carried out here a comprehensive profiling of native ganglioside mixtures extracted and purified from GBM specimens.
33900081	8	8	theme	potential	1811:1819	arg1	marker					1825:1830	a potential GBM marker	1809:1830	a potential GBM marker	1809:1830	By the occurrence of only one mobility feature and the diagnostic fragment ions, the IMS tandem MS conducted using collision-induced dissociation (CID) disclosed for the first time the presence of GT1c(d18:1/24:1) newly proposed here as a potential GBM marker.
33900081	2	9	theme	postsynthesis	522:534	arg1	process					549:555	their biosynthesis or postsynthesis modification process	500:555	process	549:555	In GBM, nearly all tumor cells exhibit aberrant cell-surface glycosylation patterns due to the alteration of their biosynthesis or postsynthesis modification process.
33900081	0	10	theme	Mass	118:121	arg1	Spectrometry					123:134	Ion Mobility Tandem Mass Spectrometry	98:134	Ion Mobility Tandem Mass Spectrometry	98:134	Gangliosides of Human Glioblastoma Multiforme: A Comprehensive Mapping and Structural Analysis by Ion Mobility Tandem Mass Spectrometry.
33900081	6	11	dep	found	1299:1303	arg1	characterized					1305:1317	characterized	1305:1317	were found characterized by a high heterogeneity in their ceramide and glycan compositions, encompassing up five Neu5Ac residues	1294:1421	The detected GGs and asialo-GGs were found characterized by a high heterogeneity in their ceramide and glycan compositions, encompassing up five Neu5Ac residues.
33900081	2	12	theme	biosynthesis	506:517	arg1	alteration					486:495	the alteration	482:495	the alteration of their biosynthesis or postsynthesis modification process	482:555	In GBM, nearly all tumor cells exhibit aberrant cell-surface glycosylation patterns due to the alteration of their biosynthesis or postsynthesis modification process.
33900081	1	13	theme	limited	354:360	arg1	options					372:378	the limited treatment options	350:378	the limited treatment options available	350:388	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	6	14	theme	Neu5Ac	1407:1412	arg1	residues					1414:1421	up five Neu5Ac residues	1399:1421	up five Neu5Ac residues	1399:1421	The detected GGs and asialo-GGs were found characterized by a high heterogeneity in their ceramide and glycan compositions, encompassing up five Neu5Ac residues.
33900081	1	15	theme	grade	200:204	arg1	tumor					215:219	a malignant, highly aggressive, grade IV brain tumor	168:219	a malignant, highly aggressive, grade IV brain tumor	168:219	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	1	15	theme	grade	200:204	arg1	multiforme					150:159	Glioblastoma multiforme	137:159	Glioblastoma multiforme (GBM)	137:165	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	2	16	theme	process	549:555	arg1	alteration					486:495	the alteration	482:495	the alteration of their biosynthesis or postsynthesis modification process	482:555	In GBM, nearly all tumor cells exhibit aberrant cell-surface glycosylation patterns due to the alteration of their biosynthesis or postsynthesis modification process.
33900081	6	17	theme	detected	1266:1273	arg1	GGs					1275:1277	GGs	1275:1277	GGs	1275:1277	The detected GGs and asialo-GGs were found characterized by a high heterogeneity in their ceramide and glycan compositions, encompassing up five Neu5Ac residues.
33900081	3	18	theme	tumor-associated	603:618	arg1	antigens					620:627	tumor-associated antigens	603:627	tumor-associated antigens	603:627	Since gangliosides (GGs) are acknowledged as tumor-associated antigens, we have carried out here a comprehensive profiling of native ganglioside mixtures extracted and purified from GBM specimens.
33900081	3	18	theme	tumor-associated	603:618	arg1	gangliosides					564:575	gangliosides	564:575	gangliosides (GGs)	564:581	Since gangliosides (GGs) are acknowledged as tumor-associated antigens, we have carried out here a comprehensive profiling of native ganglioside mixtures extracted and purified from GBM specimens.
33900081	1	19	theme	Glioblastoma	137:148	arg1	tumor					215:219	a malignant, highly aggressive, grade IV brain tumor	168:219	a malignant, highly aggressive, grade IV brain tumor	168:219	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	1	19	theme	Glioblastoma	137:148	arg1	GBM					162:164	GBM	162:164	GBM	162:164	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	1	19	theme	Glioblastoma	137:148	arg1	multiforme					150:159	Glioblastoma multiforme	137:159	Glioblastoma multiforme (GBM)	137:165	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	7	20	theme	acids	1549:1553	arg1	incidence					1524:1532	a particular incidence	1511:1532	a particular incidence of C24:1 fatty acids in the ceramide	1511:1569	The tumor was found dominated in equal and high proportions by GD3 and GT1 forms, with a particular incidence of C24:1 fatty acids in the ceramide.
33900081	4	21	theme	tumor	953:957	arg1	markers					959:965	tumor markers	953:965	tumor markers	953:965	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	5	22	theme	sialylation	1089:1099	arg1	degree					1079:1084	degree	1079:1084	degree of sialylation	1079:1099	GG separation by IMS according to the charge state, carbohydrate chain length, degree of sialylation, and ceramide composition led to the identification of no less than 160 distinct components, which represents 3-fold the number of structures identified before.
33900081	5	22	theme	sialylation	1089:1099	arg1	length					1071:1076	carbohydrate chain length	1052:1076	carbohydrate chain length	1052:1076	GG separation by IMS according to the charge state, carbohydrate chain length, degree of sialylation, and ceramide composition led to the identification of no less than 160 distinct components, which represents 3-fold the number of structures identified before.
33900081	5	22	theme	sialylation	1089:1099	arg1	composition					1115:1125	ceramide composition	1106:1125	ceramide composition	1106:1125	GG separation by IMS according to the charge state, carbohydrate chain length, degree of sialylation, and ceramide composition led to the identification of no less than 160 distinct components, which represents 3-fold the number of structures identified before.
33900081	5	22	theme	sialylation	1089:1099	arg1	state					1045:1049	the charge state	1034:1049	the charge state	1034:1049	GG separation by IMS according to the charge state, carbohydrate chain length, degree of sialylation, and ceramide composition led to the identification of no less than 160 distinct components, which represents 3-fold the number of structures identified before.
33900081	7	23	theme	C24:1	1537:1541	arg1	acids					1549:1553	C24:1 fatty acids	1537:1553	C24:1 fatty acids in the ceramide	1537:1569	The tumor was found dominated in equal and high proportions by GD3 and GT1 forms, with a particular incidence of C24:1 fatty acids in the ceramide.
33900081	2	24	theme	glycosylation	452:464	arg1	patterns					466:473	aberrant cell-surface glycosylation patterns	430:473	aberrant cell-surface glycosylation patterns due to the alteration of their biosynthesis or postsynthesis modification process	430:555	In GBM, nearly all tumor cells exhibit aberrant cell-surface glycosylation patterns due to the alteration of their biosynthesis or postsynthesis modification process.
33900081	0	25	theme	Structural	75:84	arg1	Gangliosides					0:11	Gangliosides	0:11	Gangliosides of Human Glioblastoma Multiforme: A Comprehensive Mapping and Structural Analysis by Ion Mobility Tandem Mass Spectrometry.	0:135	Gangliosides of Human Glioblastoma Multiforme: A Comprehensive Mapping and Structural Analysis by Ion Mobility Tandem Mass Spectrometry.
33900081	0	25	theme	Structural	75:84	arg1	Analysis					86:93	Structural Analysis	75:93	Structural Analysis by Ion Mobility Tandem Mass Spectrometry	75:134	Gangliosides of Human Glioblastoma Multiforme: A Comprehensive Mapping and Structural Analysis by Ion Mobility Tandem Mass Spectrometry.
33900081	1	26	theme	attention	306:314	arg1	attention					306:314	attention	306:314	attention	306:314	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	1	26	theme	attention	306:314	arg1	amount					296:301	a significant amount	282:301	a significant amount of attention	282:314	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	5	27	theme	structures	1232:1241	arg1	number					1222:1227	the number	1218:1227	the number of structures identified before	1218:1259	GG separation by IMS according to the charge state, carbohydrate chain length, degree of sialylation, and ceramide composition led to the identification of no less than 160 distinct components, which represents 3-fold the number of structures identified before.
33900081	4	28	theme	roles	944:948	arg1	discovery					879:887	the discovery	875:887	the discovery of GBM-specific structures	875:914	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	4	28	theme	roles	944:948	arg1	assessment					924:933	the assessment	920:933	the assessment of their roles as tumor markers or possible associated antigens	920:997	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	0	29	theme	Mobility	102:109	arg1	Spectrometry					123:134	Ion Mobility Tandem Mass Spectrometry	98:134	Ion Mobility Tandem Mass Spectrometry	98:134	Gangliosides of Human Glioblastoma Multiforme: A Comprehensive Mapping and Structural Analysis by Ion Mobility Tandem Mass Spectrometry.
33900081	1	30	theme	poor	331:334	arg1	prognosis					336:344	its poor prognosis	327:344	its poor prognosis	327:344	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	2	31	theme	aberrant	430:437	arg1	patterns					466:473	aberrant cell-surface glycosylation patterns	430:473	aberrant cell-surface glycosylation patterns due to the alteration of their biosynthesis or postsynthesis modification process	430:555	In GBM, nearly all tumor cells exhibit aberrant cell-surface glycosylation patterns due to the alteration of their biosynthesis or postsynthesis modification process.
33900081	8	32	theme	GT1c	1769:1772	arg1	presence					1757:1764	the presence	1753:1764	the presence of GT1c(d18:1/24:1) newly proposed here as a potential GBM marker	1753:1830	By the occurrence of only one mobility feature and the diagnostic fragment ions, the IMS tandem MS conducted using collision-induced dissociation (CID) disclosed for the first time the presence of GT1c(d18:1/24:1) newly proposed here as a potential GBM marker.
33900081	2	33	theme	due	475:477	arg1	patterns					466:473	aberrant cell-surface glycosylation patterns	430:473	aberrant cell-surface glycosylation patterns due to the alteration of their biosynthesis or postsynthesis modification process	430:555	In GBM, nearly all tumor cells exhibit aberrant cell-surface glycosylation patterns due to the alteration of their biosynthesis or postsynthesis modification process.
33900081	1	34	dep	malignant	170:178	arg1	aggressive					188:197	aggressive	188:197	aggressive	188:197	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	0	35	theme	Human	16:20	arg1	Multiforme					35:44	Human Glioblastoma Multiforme	16:44	Human Glioblastoma Multiforme	16:44	Gangliosides of Human Glioblastoma Multiforme: A Comprehensive Mapping and Structural Analysis by Ion Mobility Tandem Mass Spectrometry.
33900081	4	36	theme	associated	979:988	arg1	antigens					990:997	possible associated antigens	970:997	possible associated antigens	970:997	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	4	37	theme	separation	803:812	arg1	MS					837:838	IMS MS	833:838	IMS MS	833:838	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	4	37	theme	separation	803:812	arg1	spectrometry					819:830	high performance ion mobility separation mass spectrometry	773:830	high performance ion mobility separation mass spectrometry (IMS MS)	773:839	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	0	38	theme	Multiforme	35:44	arg1	Gangliosides					0:11	Gangliosides	0:11	Gangliosides of Human Glioblastoma Multiforme: A Comprehensive Mapping and Structural Analysis by Ion Mobility Tandem Mass Spectrometry.	0:135	Gangliosides of Human Glioblastoma Multiforme: A Comprehensive Mapping and Structural Analysis by Ion Mobility Tandem Mass Spectrometry.
33900081	0	38	theme	Multiforme	35:44	arg1	Analysis					86:93	Structural Analysis	75:93	Structural Analysis by Ion Mobility Tandem Mass Spectrometry	75:134	Gangliosides of Human Glioblastoma Multiforme: A Comprehensive Mapping and Structural Analysis by Ion Mobility Tandem Mass Spectrometry.
33900081	0	38	theme	Multiforme	35:44	arg1	Mapping					63:69	A Comprehensive Mapping	47:69	A Comprehensive Mapping	47:69	Gangliosides of Human Glioblastoma Multiforme: A Comprehensive Mapping and Structural Analysis by Ion Mobility Tandem Mass Spectrometry.
33900081	7	39	from	incidence	1524:1532	arg1	ceramide					1562:1569	the ceramide	1558:1569	the ceramide	1558:1569	The tumor was found dominated in equal and high proportions by GD3 and GT1 forms, with a particular incidence of C24:1 fatty acids in the ceramide.
33900081	4	40	theme	high	773:776	arg1	MS					837:838	IMS MS	833:838	IMS MS	833:838	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	4	40	theme	high	773:776	arg1	spectrometry					819:830	high performance ion mobility separation mass spectrometry	773:830	high performance ion mobility separation mass spectrometry (IMS MS)	773:839	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	1	41	theme	IV	206:207	arg1	tumor					215:219	a malignant, highly aggressive, grade IV brain tumor	168:219	a malignant, highly aggressive, grade IV brain tumor	168:219	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	1	41	theme	IV	206:207	arg1	multiforme					150:159	Glioblastoma multiforme	137:159	Glioblastoma multiforme (GBM)	137:165	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	7	42	from	acids	1549:1553	arg1	ceramide					1562:1569	the ceramide	1558:1569	the ceramide	1558:1569	The tumor was found dominated in equal and high proportions by GD3 and GT1 forms, with a particular incidence of C24:1 fatty acids in the ceramide.
33900081	7	43	theme	high	1467:1470	arg1	proportions					1472:1482	equal and high proportions	1457:1482	equal and high proportions	1457:1482	The tumor was found dominated in equal and high proportions by GD3 and GT1 forms, with a particular incidence of C24:1 fatty acids in the ceramide.
33900081	4	44	theme	ion	790:792	arg1	MS					837:838	IMS MS	833:838	IMS MS	833:838	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	4	44	theme	ion	790:792	arg1	spectrometry					819:830	high performance ion mobility separation mass spectrometry	773:830	high performance ion mobility separation mass spectrometry (IMS MS)	773:839	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	8	45	theme	mobility	1602:1609	arg1	feature					1611:1617	only one mobility feature	1593:1617	only one mobility feature	1593:1617	By the occurrence of only one mobility feature and the diagnostic fragment ions, the IMS tandem MS conducted using collision-induced dissociation (CID) disclosed for the first time the presence of GT1c(d18:1/24:1) newly proposed here as a potential GBM marker.
33900081	7	46	theme	equal	1457:1461	arg1	proportions					1472:1482	equal and high proportions	1457:1482	equal and high proportions	1457:1482	The tumor was found dominated in equal and high proportions by GD3 and GT1 forms, with a particular incidence of C24:1 fatty acids in the ceramide.
33900081	0	47	dep	Gangliosides	0:11	arg1	Mapping					63:69	A Comprehensive Mapping	47:69	A Comprehensive Mapping	47:69	Gangliosides of Human Glioblastoma Multiforme: A Comprehensive Mapping and Structural Analysis by Ion Mobility Tandem Mass Spectrometry.
33900081	0	47	dep	Gangliosides	0:11	arg1	Analysis					86:93	Structural Analysis	75:93	Structural Analysis by Ion Mobility Tandem Mass Spectrometry	75:134	Gangliosides of Human Glioblastoma Multiforme: A Comprehensive Mapping and Structural Analysis by Ion Mobility Tandem Mass Spectrometry.
33900081	0	47	dep	Gangliosides	0:11	arg1	Gangliosides					0:11	Gangliosides	0:11	Gangliosides of Human Glioblastoma Multiforme: A Comprehensive Mapping and Structural Analysis by Ion Mobility Tandem Mass Spectrometry.	0:135	Gangliosides of Human Glioblastoma Multiforme: A Comprehensive Mapping and Structural Analysis by Ion Mobility Tandem Mass Spectrometry.
33900081	3	48	theme	native	684:689	arg1	mixtures					703:710	native ganglioside mixtures	684:710	native ganglioside mixtures extracted and purified from GBM specimens	684:752	Since gangliosides (GGs) are acknowledged as tumor-associated antigens, we have carried out here a comprehensive profiling of native ganglioside mixtures extracted and purified from GBM specimens.
33900081	3	49	dep	carried	638:644	arg1	out					646:648	out	646:648	out	646:648	Since gangliosides (GGs) are acknowledged as tumor-associated antigens, we have carried out here a comprehensive profiling of native ganglioside mixtures extracted and purified from GBM specimens.
33900081	8	50	theme	diagnostic	1627:1636	arg1	ions					1647:1650	the diagnostic fragment ions	1623:1650	the diagnostic fragment ions	1623:1650	By the occurrence of only one mobility feature and the diagnostic fragment ions, the IMS tandem MS conducted using collision-induced dissociation (CID) disclosed for the first time the presence of GT1c(d18:1/24:1) newly proposed here as a potential GBM marker.
33900081	5	51	theme	distinct	1173:1180	arg1	components					1182:1191	no less than 160 distinct components	1156:1191	no less than 160 distinct components	1156:1191	GG separation by IMS according to the charge state, carbohydrate chain length, degree of sialylation, and ceramide composition led to the identification of no less than 160 distinct components, which represents 3-fold the number of structures identified before.
33900081	3	52	theme	mixtures	703:710	arg1	profiling					671:679	a comprehensive profiling	655:679	a comprehensive profiling of native ganglioside mixtures extracted and purified from GBM specimens	655:752	Since gangliosides (GGs) are acknowledged as tumor-associated antigens, we have carried out here a comprehensive profiling of native ganglioside mixtures extracted and purified from GBM specimens.
33900081	8	53	theme	GBM	1821:1823	arg1	marker					1825:1830	a potential GBM marker	1809:1830	a potential GBM marker	1809:1830	By the occurrence of only one mobility feature and the diagnostic fragment ions, the IMS tandem MS conducted using collision-induced dissociation (CID) disclosed for the first time the presence of GT1c(d18:1/24:1) newly proposed here as a potential GBM marker.
33900081	1	54	theme	malignant	170:178	arg1	tumor					215:219	a malignant, highly aggressive, grade IV brain tumor	168:219	a malignant, highly aggressive, grade IV brain tumor	168:219	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	1	54	theme	malignant	170:178	arg1	multiforme					150:159	Glioblastoma multiforme	137:159	Glioblastoma multiforme (GBM)	137:165	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	8	55	theme	tandem	1661:1666	arg1	MS					1668:1669	the IMS tandem MS	1653:1669	the IMS tandem MS conducted using collision-induced dissociation (CID)	1653:1722	By the occurrence of only one mobility feature and the diagnostic fragment ions, the IMS tandem MS conducted using collision-induced dissociation (CID) disclosed for the first time the presence of GT1c(d18:1/24:1) newly proposed here as a potential GBM marker.
33900081	4	56	theme	GBM-specific	892:903	arg1	structures					905:914	GBM-specific structures	892:914	GBM-specific structures	892:914	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	1	57	theme	significant	284:294	arg1	attention					306:314	attention	306:314	attention	306:314	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	1	57	theme	significant	284:294	arg1	amount					296:301	a significant amount	282:301	a significant amount of attention	282:314	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	5	58	theme	chain	1065:1069	arg1	length					1071:1076	carbohydrate chain length	1052:1076	carbohydrate chain length	1052:1076	GG separation by IMS according to the charge state, carbohydrate chain length, degree of sialylation, and ceramide composition led to the identification of no less than 160 distinct components, which represents 3-fold the number of structures identified before.
33900081	8	59	theme	collision-induced	1687:1703	arg1	CID					1719:1721	CID	1719:1721	CID	1719:1721	By the occurrence of only one mobility feature and the diagnostic fragment ions, the IMS tandem MS conducted using collision-induced dissociation (CID) disclosed for the first time the presence of GT1c(d18:1/24:1) newly proposed here as a potential GBM marker.
33900081	8	59	theme	collision-induced	1687:1703	arg1	dissociation					1705:1716	collision-induced dissociation	1687:1716	collision-induced dissociation (CID)	1687:1722	By the occurrence of only one mobility feature and the diagnostic fragment ions, the IMS tandem MS conducted using collision-induced dissociation (CID) disclosed for the first time the presence of GT1c(d18:1/24:1) newly proposed here as a potential GBM marker.
33900081	4	60	theme	structures	905:914	arg1	discovery					879:887	the discovery	875:887	the discovery of GBM-specific structures	875:914	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	4	60	theme	structures	905:914	arg1	assessment					924:933	the assessment	920:933	the assessment of their roles as tumor markers or possible associated antigens	920:997	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	1	61	theme	available	380:388	arg1	options					372:378	the limited treatment options	350:378	the limited treatment options available	350:388	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	7	62	theme	fatty	1543:1547	arg1	acids					1549:1553	C24:1 fatty acids	1537:1553	C24:1 fatty acids in the ceramide	1537:1569	The tumor was found dominated in equal and high proportions by GD3 and GT1 forms, with a particular incidence of C24:1 fatty acids in the ceramide.
33900081	2	63	theme	modification	536:547	arg1	process					549:555	their biosynthesis or postsynthesis modification process	500:555	process	549:555	In GBM, nearly all tumor cells exhibit aberrant cell-surface glycosylation patterns due to the alteration of their biosynthesis or postsynthesis modification process.
33900081	6	64	theme	glycan	1365:1370	arg1	compositions					1372:1383	their ceramide and glycan compositions	1346:1383	compositions	1372:1383	The detected GGs and asialo-GGs were found characterized by a high heterogeneity in their ceramide and glycan compositions, encompassing up five Neu5Ac residues.
33900081	5	65	theme	charge	1038:1043	arg1	state					1045:1049	the charge state	1034:1049	the charge state	1034:1049	GG separation by IMS according to the charge state, carbohydrate chain length, degree of sialylation, and ceramide composition led to the identification of no less than 160 distinct components, which represents 3-fold the number of structures identified before.
33900081	7	66	theme	particular	1513:1522	arg1	incidence					1524:1532	a particular incidence	1511:1532	a particular incidence of C24:1 fatty acids in the ceramide	1511:1569	The tumor was found dominated in equal and high proportions by GD3 and GT1 forms, with a particular incidence of C24:1 fatty acids in the ceramide.
33900081	2	67	theme	cell-surface	439:450	arg1	patterns					466:473	aberrant cell-surface glycosylation patterns	430:473	aberrant cell-surface glycosylation patterns due to the alteration of their biosynthesis or postsynthesis modification process	430:555	In GBM, nearly all tumor cells exhibit aberrant cell-surface glycosylation patterns due to the alteration of their biosynthesis or postsynthesis modification process.
33900081	0	68	theme	Ion	98:100	arg1	Spectrometry					123:134	Ion Mobility Tandem Mass Spectrometry	98:134	Ion Mobility Tandem Mass Spectrometry	98:134	Gangliosides of Human Glioblastoma Multiforme: A Comprehensive Mapping and Structural Analysis by Ion Mobility Tandem Mass Spectrometry.
33900081	5	69	theme	ceramide	1106:1113	arg1	composition					1115:1125	ceramide composition	1106:1125	ceramide composition	1106:1125	GG separation by IMS according to the charge state, carbohydrate chain length, degree of sialylation, and ceramide composition led to the identification of no less than 160 distinct components, which represents 3-fold the number of structures identified before.
33900081	7	70	from	ceramide	1562:1569	arg1	incidence					1524:1532	a particular incidence	1511:1532	a particular incidence of C24:1 fatty acids in the ceramide	1511:1569	The tumor was found dominated in equal and high proportions by GD3 and GT1 forms, with a particular incidence of C24:1 fatty acids in the ceramide.
33900081	8	71	theme	first	1742:1746	arg1	time					1748:1751	the first time	1738:1751	the first time	1738:1751	By the occurrence of only one mobility feature and the diagnostic fragment ions, the IMS tandem MS conducted using collision-induced dissociation (CID) disclosed for the first time the presence of GT1c(d18:1/24:1) newly proposed here as a potential GBM marker.
33900081	6	72	theme	high	1324:1327	arg1	heterogeneity					1329:1341	a high heterogeneity	1322:1341	a high heterogeneity in their ceramide and glycan compositions, encompassing up five Neu5Ac residues	1322:1421	The detected GGs and asialo-GGs were found characterized by a high heterogeneity in their ceramide and glycan compositions, encompassing up five Neu5Ac residues.
33900081	0	73	theme	Tandem	111:116	arg1	Spectrometry					123:134	Ion Mobility Tandem Mass Spectrometry	98:134	Ion Mobility Tandem Mass Spectrometry	98:134	Gangliosides of Human Glioblastoma Multiforme: A Comprehensive Mapping and Structural Analysis by Ion Mobility Tandem Mass Spectrometry.
33900081	6	74	from	heterogeneity	1329:1341	arg1	ceramide					1352:1359	their ceramide and glycan compositions	1346:1383	ceramide	1352:1359	The detected GGs and asialo-GGs were found characterized by a high heterogeneity in their ceramide and glycan compositions, encompassing up five Neu5Ac residues.
33900081	6	74	from	heterogeneity	1329:1341	arg1	compositions					1372:1383	their ceramide and glycan compositions	1346:1383	compositions	1372:1383	The detected GGs and asialo-GGs were found characterized by a high heterogeneity in their ceramide and glycan compositions, encompassing up five Neu5Ac residues.
33900081	0	75	theme	Glioblastoma	22:33	arg1	Multiforme					35:44	Human Glioblastoma Multiforme	16:44	Human Glioblastoma Multiforme	16:44	Gangliosides of Human Glioblastoma Multiforme: A Comprehensive Mapping and Structural Analysis by Ion Mobility Tandem Mass Spectrometry.
33900081	1	76	theme	nearby	257:262	arg1	tissue					264:269	the nearby tissue	253:269	the nearby tissue	253:269	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	5	77	theme	GG	1000:1001	arg1	separation					1003:1012	GG separation	1000:1012	GG separation by IMS according to the charge state, carbohydrate chain length, degree of sialylation, and ceramide composition	1000:1125	GG separation by IMS according to the charge state, carbohydrate chain length, degree of sialylation, and ceramide composition led to the identification of no less than 160 distinct components, which represents 3-fold the number of structures identified before.
33900081	4	78	theme	possible	970:977	arg1	antigens					990:997	possible associated antigens	970:997	possible associated antigens	970:997	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	4	79	theme	mobility	794:801	arg1	MS					837:838	IMS MS	833:838	IMS MS	833:838	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	4	79	theme	mobility	794:801	arg1	spectrometry					819:830	high performance ion mobility separation mass spectrometry	773:830	high performance ion mobility separation mass spectrometry (IMS MS)	773:839	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	0	80	theme	Comprehensive	49:61	arg1	Mapping					63:69	A Comprehensive Mapping	47:69	A Comprehensive Mapping	47:69	Gangliosides of Human Glioblastoma Multiforme: A Comprehensive Mapping and Structural Analysis by Ion Mobility Tandem Mass Spectrometry.
33900081	0	80	theme	Comprehensive	49:61	arg1	Gangliosides					0:11	Gangliosides	0:11	Gangliosides of Human Glioblastoma Multiforme: A Comprehensive Mapping and Structural Analysis by Ion Mobility Tandem Mass Spectrometry.	0:135	Gangliosides of Human Glioblastoma Multiforme: A Comprehensive Mapping and Structural Analysis by Ion Mobility Tandem Mass Spectrometry.
33900081	3	81	dep	mixtures	703:710	arg1	purified					726:733	purified	726:733	purified from GBM specimens	726:752	Since gangliosides (GGs) are acknowledged as tumor-associated antigens, we have carried out here a comprehensive profiling of native ganglioside mixtures extracted and purified from GBM specimens.
33900081	3	81	dep	mixtures	703:710	arg1	extracted					712:720	extracted	712:720	extracted	712:720	Since gangliosides (GGs) are acknowledged as tumor-associated antigens, we have carried out here a comprehensive profiling of native ganglioside mixtures extracted and purified from GBM specimens.
33900081	7	82	theme	GT1	1495:1497	arg1	forms					1499:1503	GT1 forms	1495:1503	GT1 forms	1495:1503	The tumor was found dominated in equal and high proportions by GD3 and GT1 forms, with a particular incidence of C24:1 fatty acids in the ceramide.
33900081	2	83	theme	tumor	410:414	arg1	cells					416:420	nearly all tumor cells	399:420	nearly all tumor cells	399:420	In GBM, nearly all tumor cells exhibit aberrant cell-surface glycosylation patterns due to the alteration of their biosynthesis or postsynthesis modification process.
33900081	4	84	theme	mass	814:817	arg1	MS					837:838	IMS MS	833:838	IMS MS	833:838	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	4	84	theme	mass	814:817	arg1	spectrometry					819:830	high performance ion mobility separation mass spectrometry	773:830	high performance ion mobility separation mass spectrometry (IMS MS)	773:839	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	5	85	theme	carbohydrate	1052:1063	arg1	length					1071:1076	carbohydrate chain length	1052:1076	carbohydrate chain length	1052:1076	GG separation by IMS according to the charge state, carbohydrate chain length, degree of sialylation, and ceramide composition led to the identification of no less than 160 distinct components, which represents 3-fold the number of structures identified before.
33900081	7	86	dep	found	1438:1442	arg1	dominated					1444:1452	dominated	1444:1452	dominated	1444:1452	The tumor was found dominated in equal and high proportions by GD3 and GT1 forms, with a particular incidence of C24:1 fatty acids in the ceramide.
33900081	8	87	theme	feature	1611:1617	arg1	ions					1647:1650	the diagnostic fragment ions	1623:1650	the diagnostic fragment ions	1623:1650	By the occurrence of only one mobility feature and the diagnostic fragment ions, the IMS tandem MS conducted using collision-induced dissociation (CID) disclosed for the first time the presence of GT1c(d18:1/24:1) newly proposed here as a potential GBM marker.
33900081	8	87	theme	feature	1611:1617	arg1	occurrence					1579:1588	the occurrence	1575:1588	the occurrence of only one mobility feature	1575:1617	By the occurrence of only one mobility feature and the diagnostic fragment ions, the IMS tandem MS conducted using collision-induced dissociation (CID) disclosed for the first time the presence of GT1c(d18:1/24:1) newly proposed here as a potential GBM marker.
33900081	1	88	theme	brain	209:213	arg1	tumor					215:219	a malignant, highly aggressive, grade IV brain tumor	168:219	a malignant, highly aggressive, grade IV brain tumor	168:219	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	1	88	theme	brain	209:213	arg1	multiforme					150:159	Glioblastoma multiforme	137:159	Glioblastoma multiforme (GBM)	137:165	Glioblastoma multiforme (GBM), a malignant, highly aggressive, grade IV brain tumor, which rapidly infiltrates into the nearby tissue, has drawn a significant amount of attention because of its poor prognosis and the limited treatment options available.
33900081	4	89	theme	performance	778:788	arg1	MS					837:838	IMS MS	833:838	IMS MS	833:838	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
33900081	4	89	theme	performance	778:788	arg1	spectrometry					819:830	high performance ion mobility separation mass spectrometry	773:830	high performance ion mobility separation mass spectrometry (IMS MS)	773:839	For this purpose, high performance ion mobility separation mass spectrometry (IMS MS) was thoroughly optimized to allow the discovery of GBM-specific structures and the assessment of their roles as tumor markers or possible associated antigens.
32120129	5	0	theme	sequential	1192:1201	arg1	pH					1203:1204	sequential pH	1192:1204	sequential pH	1192:1204	In vitro dissolution test indicates the great possibility of programming the multi-staged degradation of such composites in response to sequential pH or thermal stimuli.
32120129	2	1	theme	ideal	306:310	arg1	candidates					312:321	the ideal candidates	302:321	the ideal candidates	302:321	However, most of the ideal candidates often suffer from non-biodegradation, weak mechanical strength and random macro-porous structure, which do limit the creativity and dynamism of water-sensitive SPMs.
32120129	8	2	theme	straightforward	1616:1630	arg1	approach					1644:1651	such straightforward fabrication approach	1611:1651	such straightforward fabrication approach	1611:1651	Thus, coupling of such straightforward fabrication approach, multifunctionality and biodegradable prerequisite feature indicates great potential for use in the minimally invasive surgery.
32120129	6	3	theme	porous	1239:1244	arg1	structure					1255:1263	the porous internal structure	1235:1263	the porous internal structure	1235:1263	Based on the porous internal structure, we also demonstrate some potential applications of the hydrogels for assembly of nanomaterials, such as Au nanorods for remote sensing system and model protein of insulin for drug controlled release.
32120129	8	4	theme	prerequisite	1691:1702	arg1	feature					1704:1710	biodegradable prerequisite feature	1677:1710	biodegradable prerequisite feature	1677:1710	Thus, coupling of such straightforward fabrication approach, multifunctionality and biodegradable prerequisite feature indicates great potential for use in the minimally invasive surgery.
32120129	8	5	from	use	1742:1744	arg1	surgery					1772:1778	the minimally invasive surgery	1749:1778	the minimally invasive surgery	1749:1778	Thus, coupling of such straightforward fabrication approach, multifunctionality and biodegradable prerequisite feature indicates great potential for use in the minimally invasive surgery.
32120129	3	6	theme	thermoplastic	586:598	arg1	matrix					625:630	a thermoplastic elastomer agarose (Agar) matrix	584:630	a thermoplastic elastomer agarose (Agar) matrix	584:630	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	4	7	theme	memory	911:916	arg1	property					918:925	such shape memory property	900:925	such shape memory property	900:925	Results showed that such shape memory property is erasable and pH-dependent which achieves a great shape memory property at high pH and then can be erased in acidic condition.
32120129	6	8	theme	hydrogels	1321:1329	arg1	applications					1301:1312	some potential applications	1286:1312	some potential applications	1286:1312	Based on the porous internal structure, we also demonstrate some potential applications of the hydrogels for assembly of nanomaterials, such as Au nanorods for remote sensing system and model protein of insulin for drug controlled release.
32120129	6	8	theme	hydrogels	1321:1329	arg1	nanorods					1373:1380	Au nanorods	1370:1380	Au nanorods for remote sensing system and model protein of insulin for drug controlled release	1370:1463	Based on the porous internal structure, we also demonstrate some potential applications of the hydrogels for assembly of nanomaterials, such as Au nanorods for remote sensing system and model protein of insulin for drug controlled release.
32120129	5	9	theme	In	1056:1057	arg1	test					1077:1080	In vitro dissolution test	1056:1080	In vitro dissolution test	1056:1080	In vitro dissolution test indicates the great possibility of programming the multi-staged degradation of such composites in response to sequential pH or thermal stimuli.
32120129	6	10	theme	model	1412:1416	arg1	protein					1418:1424	model protein	1412:1424	model protein	1412:1424	Based on the porous internal structure, we also demonstrate some potential applications of the hydrogels for assembly of nanomaterials, such as Au nanorods for remote sensing system and model protein of insulin for drug controlled release.
32120129	6	11	theme	potential	1291:1299	arg1	applications					1301:1312	some potential applications	1286:1312	some potential applications	1286:1312	Based on the porous internal structure, we also demonstrate some potential applications of the hydrogels for assembly of nanomaterials, such as Au nanorods for remote sensing system and model protein of insulin for drug controlled release.
32120129	6	11	theme	potential	1291:1299	arg1	nanorods					1373:1380	Au nanorods	1370:1380	Au nanorods for remote sensing system and model protein of insulin for drug controlled release	1370:1463	Based on the porous internal structure, we also demonstrate some potential applications of the hydrogels for assembly of nanomaterials, such as Au nanorods for remote sensing system and model protein of insulin for drug controlled release.
32120129	3	12	theme	agarose	610:616	arg1	matrix					625:630	a thermoplastic elastomer agarose (Agar) matrix	584:630	a thermoplastic elastomer agarose (Agar) matrix	584:630	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	1	13	theme	Water-induced	157:169	arg1	SMPs					194:197	SMPs	194:197	SMPs	194:197	Water-induced shape memory polymers (SMPs) may have important applications in the fields of tissue engineering and biomedicine.
32120129	1	13	theme	Water-induced	157:169	arg1	polymers					184:191	Water-induced shape memory polymers	157:191	Water-induced shape memory polymers (SMPs)	157:198	Water-induced shape memory polymers (SMPs) may have important applications in the fields of tissue engineering and biomedicine.
32120129	3	14	theme	response	857:864	arg1	min					874:876	response time <1 min	857:876	response time <1 min	857:876	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	3	14	theme	response	857:864	arg1	%					854:854	>90 %	850:854	>90 %	850:854	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	4	15	theme	such	900:903	arg1	property					918:925	such shape memory property	900:925	such shape memory property	900:925	Results showed that such shape memory property is erasable and pH-dependent which achieves a great shape memory property at high pH and then can be erased in acidic condition.
32120129	3	16	theme	water-induced	814:826	arg1	ratio					843:847	water-induced shape recovery ratio	814:847	water-induced shape recovery ratio (>90 %, response time <1 min)	814:877	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	8	17	theme	invasive	1763:1770	arg1	surgery					1772:1778	the minimally invasive surgery	1749:1778	the minimally invasive surgery	1749:1778	Thus, coupling of such straightforward fabrication approach, multifunctionality and biodegradable prerequisite feature indicates great potential for use in the minimally invasive surgery.
32120129	1	18	theme	memory	177:182	arg1	SMPs					194:197	SMPs	194:197	SMPs	194:197	Water-induced shape memory polymers (SMPs) may have important applications in the fields of tissue engineering and biomedicine.
32120129	1	18	theme	memory	177:182	arg1	polymers					184:191	Water-induced shape memory polymers	157:191	Water-induced shape memory polymers (SMPs)	157:198	Water-induced shape memory polymers (SMPs) may have important applications in the fields of tissue engineering and biomedicine.
32120129	8	19	theme	approach	1644:1651	arg1	coupling					1599:1606	coupling	1599:1606	coupling of such straightforward fabrication approach, multifunctionality and biodegradable prerequisite feature	1599:1710	Thus, coupling of such straightforward fabrication approach, multifunctionality and biodegradable prerequisite feature indicates great potential for use in the minimally invasive surgery.
32120129	6	20	theme	Au	1370:1371	arg1	nanorods					1373:1380	Au nanorods	1370:1380	Au nanorods for remote sensing system and model protein of insulin for drug controlled release	1370:1463	Based on the porous internal structure, we also demonstrate some potential applications of the hydrogels for assembly of nanomaterials, such as Au nanorods for remote sensing system and model protein of insulin for drug controlled release.
32120129	5	21	theme	great	1096:1100	arg1	possibility					1102:1112	the great possibility	1092:1112	the great possibility of programming	1092:1127	In vitro dissolution test indicates the great possibility of programming the multi-staged degradation of such composites in response to sequential pH or thermal stimuli.
32120129	7	22	theme	polymer	1545:1551	arg1	use					1525:1527	the use	1521:1527	the use of most natural polymer including gelatin, alginate, and so on	1521:1590	Moreover, the process proved to be versatile, allowing the use of most natural polymer including gelatin, alginate, and so on.
32120129	4	23	theme	shape	979:983	arg1	property					992:999	a great shape memory property	971:999	a great shape memory property	971:999	Results showed that such shape memory property is erasable and pH-dependent which achieves a great shape memory property at high pH and then can be erased in acidic condition.
32120129	0	24	theme	memory	99:104	arg1	property					106:113	adjustable water-sensitive shape memory property	66:113	adjustable water-sensitive shape memory property	66:113	Ice-templating of chitosan/agarose porous composite hydrogel with adjustable water-sensitive shape memory property and multi-staged degradation performance.
32120129	3	25	theme	<1	871:872	arg1	min					874:876	response time <1 min	857:876	response time <1 min	857:876	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	3	25	theme	<1	871:872	arg1	%					854:854	>90 %	850:854	>90 %	850:854	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	7	26	theme	most	1532:1535	arg1	polymer					1545:1551	most natural polymer	1532:1551	most natural polymer including gelatin, alginate, and so on	1532:1590	Moreover, the process proved to be versatile, allowing the use of most natural polymer including gelatin, alginate, and so on.
32120129	3	27	theme	recovery	834:841	arg1	ratio					843:847	water-induced shape recovery ratio	814:847	water-induced shape recovery ratio (>90 %, response time <1 min)	814:877	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	6	28	theme	nanomaterials	1347:1359	arg1	assembly					1335:1342	assembly	1335:1342	assembly of nanomaterials	1335:1359	Based on the porous internal structure, we also demonstrate some potential applications of the hydrogels for assembly of nanomaterials, such as Au nanorods for remote sensing system and model protein of insulin for drug controlled release.
32120129	5	29	theme	dissolution	1065:1075	arg1	test					1077:1080	In vitro dissolution test	1056:1080	In vitro dissolution test	1056:1080	In vitro dissolution test indicates the great possibility of programming the multi-staged degradation of such composites in response to sequential pH or thermal stimuli.
32120129	0	30	theme	degradation	132:142	arg1	performance					144:154	multi-staged degradation performance	119:154	multi-staged degradation performance	119:154	Ice-templating of chitosan/agarose porous composite hydrogel with adjustable water-sensitive shape memory property and multi-staged degradation performance.
32120129	3	31	contain	has	776:778	arg2	%					807:807	>95 %	803:807	>95 %	803:807	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	3	31	contain	has	776:778	arg1	which					770:774	which	770:774	which	770:774	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	3	31	contain	has	776:778	arg2	ratio					843:847	water-induced shape recovery ratio	814:847	water-induced shape recovery ratio (>90 %, response time <1 min)	814:877	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	3	31	contain	has	776:778	arg2	porosity					793:800	a ultra-high porosity	780:800	a ultra-high porosity (>95 %)	780:808	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	5	32	theme	such	1161:1164	arg1	composites					1166:1175	such composites	1161:1175	such composites	1161:1175	In vitro dissolution test indicates the great possibility of programming the multi-staged degradation of such composites in response to sequential pH or thermal stimuli.
32120129	2	33	theme	water-sensitive	467:481	arg1	SPMs					483:486	water-sensitive SPMs	467:486	water-sensitive SPMs	467:486	However, most of the ideal candidates often suffer from non-biodegradation, weak mechanical strength and random macro-porous structure, which do limit the creativity and dynamism of water-sensitive SPMs.
32120129	3	34	theme	above	500:504	arg1	issue					506:510	above issue	500:510	above issue	500:510	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	0	35	theme	chitosan/agarose	18:33	arg1	hydrogel					52:59	chitosan/agarose porous composite hydrogel	18:59	chitosan/agarose porous composite hydrogel	18:59	Ice-templating of chitosan/agarose porous composite hydrogel with adjustable water-sensitive shape memory property and multi-staged degradation performance.
32120129	3	36	theme	dual-responsive	660:674	arg1	hydrogel					709:716	a novel dual-responsive interpenetrating polymer network hydrogel	652:716	a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure	652:745	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	2	37	dep	creativity	440:449	arg1	the					436:438	the	436:438	the	436:438	However, most of the ideal candidates often suffer from non-biodegradation, weak mechanical strength and random macro-porous structure, which do limit the creativity and dynamism of water-sensitive SPMs.
32120129	0	38	theme	composite	42:50	arg1	hydrogel					52:59	chitosan/agarose porous composite hydrogel	18:59	chitosan/agarose porous composite hydrogel	18:59	Ice-templating of chitosan/agarose porous composite hydrogel with adjustable water-sensitive shape memory property and multi-staged degradation performance.
32120129	6	39	theme	sensing	1393:1399	arg1	system					1401:1406	remote sensing system	1386:1406	remote sensing system	1386:1406	Based on the porous internal structure, we also demonstrate some potential applications of the hydrogels for assembly of nanomaterials, such as Au nanorods for remote sensing system and model protein of insulin for drug controlled release.
32120129	4	40	theme	high	1004:1007	arg1	pH					1009:1010	high pH	1004:1010	high pH	1004:1010	Results showed that such shape memory property is erasable and pH-dependent which achieves a great shape memory property at high pH and then can be erased in acidic condition.
32120129	0	41	theme	water-sensitive	77:91	arg1	property					106:113	adjustable water-sensitive shape memory property	66:113	adjustable water-sensitive shape memory property	66:113	Ice-templating of chitosan/agarose porous composite hydrogel with adjustable water-sensitive shape memory property and multi-staged degradation performance.
32120129	0	42	with	Ice-templating	0:13	arg1	property					106:113	adjustable water-sensitive shape memory property	66:113	adjustable water-sensitive shape memory property	66:113	Ice-templating of chitosan/agarose porous composite hydrogel with adjustable water-sensitive shape memory property and multi-staged degradation performance.
32120129	0	42	with	Ice-templating	0:13	arg1	performance					144:154	multi-staged degradation performance	119:154	multi-staged degradation performance	119:154	Ice-templating of chitosan/agarose porous composite hydrogel with adjustable water-sensitive shape memory property and multi-staged degradation performance.
32120129	2	43	theme	weak	361:364	arg1	strength					377:384	weak mechanical strength	361:384	weak mechanical strength	361:384	However, most of the ideal candidates often suffer from non-biodegradation, weak mechanical strength and random macro-porous structure, which do limit the creativity and dynamism of water-sensitive SPMs.
32120129	5	44	theme	thermal	1209:1215	arg1	stimuli					1217:1223	thermal stimuli	1209:1223	thermal stimuli	1209:1223	In vitro dissolution test indicates the great possibility of programming the multi-staged degradation of such composites in response to sequential pH or thermal stimuli.
32120129	3	45	theme	polymer	693:699	arg1	hydrogel					709:716	a novel dual-responsive interpenetrating polymer network hydrogel	652:716	a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure	652:745	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	6	46	theme	remote	1386:1391	arg1	system					1401:1406	remote sensing system	1386:1406	remote sensing system	1386:1406	Based on the porous internal structure, we also demonstrate some potential applications of the hydrogels for assembly of nanomaterials, such as Au nanorods for remote sensing system and model protein of insulin for drug controlled release.
32120129	2	47	theme	random	390:395	arg1	structure					410:418	random macro-porous structure	390:418	random macro-porous structure	390:418	However, most of the ideal candidates often suffer from non-biodegradation, weak mechanical strength and random macro-porous structure, which do limit the creativity and dynamism of water-sensitive SPMs.
32120129	6	48	theme	drug	1441:1444	arg1	release					1457:1463	drug controlled release	1441:1463	drug controlled release	1441:1463	Based on the porous internal structure, we also demonstrate some potential applications of the hydrogels for assembly of nanomaterials, such as Au nanorods for remote sensing system and model protein of insulin for drug controlled release.
32120129	4	49	theme	acidic	1038:1043	arg1	condition					1045:1053	acidic condition	1038:1053	acidic condition	1038:1053	Results showed that such shape memory property is erasable and pH-dependent which achieves a great shape memory property at high pH and then can be erased in acidic condition.
32120129	6	50	theme	insulin	1429:1435	arg1	system					1401:1406	remote sensing system	1386:1406	remote sensing system	1386:1406	Based on the porous internal structure, we also demonstrate some potential applications of the hydrogels for assembly of nanomaterials, such as Au nanorods for remote sensing system and model protein of insulin for drug controlled release.
32120129	6	50	theme	insulin	1429:1435	arg1	protein					1418:1424	model protein	1412:1424	model protein	1412:1424	Based on the porous internal structure, we also demonstrate some potential applications of the hydrogels for assembly of nanomaterials, such as Au nanorods for remote sensing system and model protein of insulin for drug controlled release.
32120129	8	51	theme	biodegradable	1677:1689	arg1	feature					1704:1710	biodegradable prerequisite feature	1677:1710	biodegradable prerequisite feature	1677:1710	Thus, coupling of such straightforward fabrication approach, multifunctionality and biodegradable prerequisite feature indicates great potential for use in the minimally invasive surgery.
32120129	6	52	theme	internal	1246:1253	arg1	structure					1255:1263	the porous internal structure	1235:1263	the porous internal structure	1235:1263	Based on the porous internal structure, we also demonstrate some potential applications of the hydrogels for assembly of nanomaterials, such as Au nanorods for remote sensing system and model protein of insulin for drug controlled release.
32120129	3	53	theme	pH-responsive	549:561	arg1	CHIT					573:576	CHIT	573:576	CHIT	573:576	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	3	53	theme	pH-responsive	549:561	arg1	chitosan					563:570	the pH-responsive chitosan	545:570	the pH-responsive chitosan (CHIT)	545:577	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	8	54	theme	multifunctionality	1654:1671	arg1	coupling					1599:1606	coupling	1599:1606	coupling of such straightforward fabrication approach, multifunctionality and biodegradable prerequisite feature	1599:1710	Thus, coupling of such straightforward fabrication approach, multifunctionality and biodegradable prerequisite feature indicates great potential for use in the minimally invasive surgery.
32120129	3	55	with	hydrogel	709:716	arg1	structure					737:745	highly porous structure	723:745	highly porous structure	723:745	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	1	56	theme	important	209:217	arg1	applications					219:230	important applications	209:230	important applications	209:230	Water-induced shape memory polymers (SMPs) may have important applications in the fields of tissue engineering and biomedicine.
32120129	3	57	theme	ultra-high	782:791	arg1	porosity					793:800	a ultra-high porosity	780:800	a ultra-high porosity (>95 %)	780:808	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	3	57	theme	ultra-high	782:791	arg1	%					807:807	>95 %	803:807	>95 %	803:807	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	8	58	theme	feature	1704:1710	arg1	coupling					1599:1606	coupling	1599:1606	coupling of such straightforward fabrication approach, multifunctionality and biodegradable prerequisite feature	1599:1710	Thus, coupling of such straightforward fabrication approach, multifunctionality and biodegradable prerequisite feature indicates great potential for use in the minimally invasive surgery.
32120129	4	59	theme	shape	905:909	arg1	property					918:925	such shape memory property	900:925	such shape memory property	900:925	Results showed that such shape memory property is erasable and pH-dependent which achieves a great shape memory property at high pH and then can be erased in acidic condition.
32120129	8	60	theme	great	1722:1726	arg1	potential					1728:1736	great potential	1722:1736	great potential for use in the minimally invasive surgery	1722:1778	Thus, coupling of such straightforward fabrication approach, multifunctionality and biodegradable prerequisite feature indicates great potential for use in the minimally invasive surgery.
32120129	3	61	theme	elastomer	600:608	arg1	matrix					625:630	a thermoplastic elastomer agarose (Agar) matrix	584:630	a thermoplastic elastomer agarose (Agar) matrix	584:630	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	5	62	dep	pH	1203:1204	arg1	response					1180:1187	response	1180:1187	response	1180:1187	In vitro dissolution test indicates the great possibility of programming the multi-staged degradation of such composites in response to sequential pH or thermal stimuli.
32120129	3	63	dep	ratio	843:847	arg1	min					874:876	response time <1 min	857:876	response time <1 min	857:876	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	3	63	dep	ratio	843:847	arg1	%					854:854	>90 %	850:854	>90 %	850:854	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	1	64	theme	shape	171:175	arg1	SMPs					194:197	SMPs	194:197	SMPs	194:197	Water-induced shape memory polymers (SMPs) may have important applications in the fields of tissue engineering and biomedicine.
32120129	1	64	theme	shape	171:175	arg1	polymers					184:191	Water-induced shape memory polymers	157:191	Water-induced shape memory polymers (SMPs)	157:198	Water-induced shape memory polymers (SMPs) may have important applications in the fields of tissue engineering and biomedicine.
32120129	3	65	theme	time	866:869	arg1	min					874:876	response time <1 min	857:876	response time <1 min	857:876	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	3	65	theme	time	866:869	arg1	%					854:854	>90 %	850:854	>90 %	850:854	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	3	66	theme	shape	828:832	arg1	ratio					843:847	water-induced shape recovery ratio	814:847	water-induced shape recovery ratio (>90 %, response time <1 min)	814:877	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	0	67	theme	shape	93:97	arg1	property					106:113	adjustable water-sensitive shape memory property	66:113	adjustable water-sensitive shape memory property	66:113	Ice-templating of chitosan/agarose porous composite hydrogel with adjustable water-sensitive shape memory property and multi-staged degradation performance.
32120129	7	68	theme	natural	1537:1543	arg1	polymer					1545:1551	most natural polymer	1532:1551	most natural polymer including gelatin, alginate, and so on	1532:1590	Moreover, the process proved to be versatile, allowing the use of most natural polymer including gelatin, alginate, and so on.
32120129	1	69	theme	tissue	249:254	arg1	engineering					256:266	tissue engineering	249:266	tissue engineering	249:266	Water-induced shape memory polymers (SMPs) may have important applications in the fields of tissue engineering and biomedicine.
32120129	4	70	theme	great	973:977	arg1	property					992:999	a great shape memory property	971:999	a great shape memory property	971:999	Results showed that such shape memory property is erasable and pH-dependent which achieves a great shape memory property at high pH and then can be erased in acidic condition.
32120129	0	71	theme	multi-staged	119:130	arg1	performance					144:154	multi-staged degradation performance	119:154	multi-staged degradation performance	119:154	Ice-templating of chitosan/agarose porous composite hydrogel with adjustable water-sensitive shape memory property and multi-staged degradation performance.
32120129	5	72	theme	programming	1117:1127	arg1	possibility					1102:1112	the great possibility	1092:1112	the great possibility of programming	1092:1127	In vitro dissolution test indicates the great possibility of programming the multi-staged degradation of such composites in response to sequential pH or thermal stimuli.
32120129	2	73	theme	SPMs	483:486	arg1	creativity					440:449	creativity	440:449	creativity	440:449	However, most of the ideal candidates often suffer from non-biodegradation, weak mechanical strength and random macro-porous structure, which do limit the creativity and dynamism of water-sensitive SPMs.
32120129	2	73	theme	SPMs	483:486	arg1	dynamism					455:462	dynamism	455:462	dynamism	455:462	However, most of the ideal candidates often suffer from non-biodegradation, weak mechanical strength and random macro-porous structure, which do limit the creativity and dynamism of water-sensitive SPMs.
32120129	0	74	theme	porous	35:40	arg1	hydrogel					52:59	chitosan/agarose porous composite hydrogel	18:59	chitosan/agarose porous composite hydrogel	18:59	Ice-templating of chitosan/agarose porous composite hydrogel with adjustable water-sensitive shape memory property and multi-staged degradation performance.
32120129	5	75	theme	composites	1166:1175	arg1	degradation					1146:1156	the multi-staged degradation	1129:1156	the multi-staged degradation of such composites	1129:1175	In vitro dissolution test indicates the great possibility of programming the multi-staged degradation of such composites in response to sequential pH or thermal stimuli.
32120129	3	76	theme	novel	654:658	arg1	hydrogel					709:716	a novel dual-responsive interpenetrating polymer network hydrogel	652:716	a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure	652:745	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	0	77	theme	hydrogel	52:59	arg1	Ice-templating					0:13	Ice-templating	0:13	Ice-templating of chitosan/agarose porous composite hydrogel with adjustable water-sensitive shape memory property and multi-staged degradation performance.	0:155	Ice-templating of chitosan/agarose porous composite hydrogel with adjustable water-sensitive shape memory property and multi-staged degradation performance.
32120129	4	78	theme	memory	985:990	arg1	property					992:999	a great shape memory property	971:999	a great shape memory property	971:999	Results showed that such shape memory property is erasable and pH-dependent which achieves a great shape memory property at high pH and then can be erased in acidic condition.
32120129	1	79	contain	have	204:207	arg1	SMPs					194:197	SMPs	194:197	SMPs	194:197	Water-induced shape memory polymers (SMPs) may have important applications in the fields of tissue engineering and biomedicine.
32120129	1	79	contain	have	204:207	arg2	applications					219:230	important applications	209:230	important applications	209:230	Water-induced shape memory polymers (SMPs) may have important applications in the fields of tissue engineering and biomedicine.
32120129	1	79	contain	have	204:207	arg1	polymers					184:191	Water-induced shape memory polymers	157:191	Water-induced shape memory polymers (SMPs)	157:198	Water-induced shape memory polymers (SMPs) may have important applications in the fields of tissue engineering and biomedicine.
32120129	0	80	theme	adjustable	66:75	arg1	property					106:113	adjustable water-sensitive shape memory property	66:113	adjustable water-sensitive shape memory property	66:113	Ice-templating of chitosan/agarose porous composite hydrogel with adjustable water-sensitive shape memory property and multi-staged degradation performance.
32120129	5	81	theme	multi-staged	1133:1144	arg1	degradation					1146:1156	the multi-staged degradation	1129:1156	the multi-staged degradation of such composites	1129:1175	In vitro dissolution test indicates the great possibility of programming the multi-staged degradation of such composites in response to sequential pH or thermal stimuli.
32120129	3	82	theme	network	701:707	arg1	hydrogel					709:716	a novel dual-responsive interpenetrating polymer network hydrogel	652:716	a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure	652:745	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	2	83	theme	mechanical	366:375	arg1	strength					377:384	weak mechanical strength	361:384	weak mechanical strength	361:384	However, most of the ideal candidates often suffer from non-biodegradation, weak mechanical strength and random macro-porous structure, which do limit the creativity and dynamism of water-sensitive SPMs.
32120129	3	84	theme	Agar	619:622	arg1	matrix					625:630	a thermoplastic elastomer agarose (Agar) matrix	584:630	a thermoplastic elastomer agarose (Agar) matrix	584:630	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	5	85	dep	In	1056:1057	arg1	vitro					1059:1063	vitro	1059:1063	vitro	1059:1063	In vitro dissolution test indicates the great possibility of programming the multi-staged degradation of such composites in response to sequential pH or thermal stimuli.
32120129	3	86	theme	interpenetrating	676:691	arg1	hydrogel					709:716	a novel dual-responsive interpenetrating polymer network hydrogel	652:716	a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure	652:745	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	8	87	theme	such	1611:1614	arg1	approach					1644:1651	such straightforward fabrication approach	1611:1651	such straightforward fabrication approach	1611:1651	Thus, coupling of such straightforward fabrication approach, multifunctionality and biodegradable prerequisite feature indicates great potential for use in the minimally invasive surgery.
32120129	1	88	theme	engineering	256:266	arg1	fields					239:244	the fields	235:244	the fields of tissue engineering and biomedicine	235:282	Water-induced shape memory polymers (SMPs) may have important applications in the fields of tissue engineering and biomedicine.
32120129	6	89	theme	controlled	1446:1455	arg1	release					1457:1463	drug controlled release	1441:1463	drug controlled release	1441:1463	Based on the porous internal structure, we also demonstrate some potential applications of the hydrogels for assembly of nanomaterials, such as Au nanorods for remote sensing system and model protein of insulin for drug controlled release.
32120129	3	90	theme	porous	730:735	arg1	structure					737:745	highly porous structure	723:745	highly porous structure	723:745	To address above issue, in this study, by incorporating the pH-responsive chitosan (CHIT) into a thermoplastic elastomer agarose (Agar) matrix and ice-templating, a novel dual-responsive interpenetrating polymer network hydrogel with highly porous structure was facilely prepared, which has a ultra-high porosity (>95 %) and water-induced shape recovery ratio (>90 %, response time <1 min).
32120129	2	91	theme	macro-porous	397:408	arg1	structure					410:418	random macro-porous structure	390:418	random macro-porous structure	390:418	However, most of the ideal candidates often suffer from non-biodegradation, weak mechanical strength and random macro-porous structure, which do limit the creativity and dynamism of water-sensitive SPMs.
32120129	8	92	theme	fabrication	1632:1642	arg1	approach					1644:1651	such straightforward fabrication approach	1611:1651	such straightforward fabrication approach	1611:1651	Thus, coupling of such straightforward fabrication approach, multifunctionality and biodegradable prerequisite feature indicates great potential for use in the minimally invasive surgery.
32120129	1	93	theme	biomedicine	272:282	arg1	fields					239:244	the fields	235:244	the fields of tissue engineering and biomedicine	235:282	Water-induced shape memory polymers (SMPs) may have important applications in the fields of tissue engineering and biomedicine.
34294293	3	0	theme	agarose	370:376	arg1	oligosaccharides					378:393	agarose oligosaccharides	370:393	agarose oligosaccharides (AGO) obtained from marine red algae	370:430	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	3	0	theme	agarose	370:376	arg1	a					445:445	a	445:445	a	445:445	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	3	0	theme	agarose	370:376	arg1	stabilizer					460:469	stabilizer	460:469	stabilizer	460:469	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	4	1	theme	electron	691:698	arg1	TEM					712:714	TEM	712:714	TEM	712:714	Transmission electron microscopy (TEM) and Malvern particle size analyzer showed that AGO-AgNPs-OA was spherical or elliptic with average size of about 100 nm.
34294293	4	1	theme	electron	691:698	arg1	microscopy					700:709	Transmission electron microscopy	678:709	Transmission electron microscopy (TEM)	678:715	Transmission electron microscopy (TEM) and Malvern particle size analyzer showed that AGO-AgNPs-OA was spherical or elliptic with average size of about 100 nm.
34294293	7	2	theme	marine	1129:1134	arg1	oligosaccharides					1136:1151	AMPs conjugated marine oligosaccharides	1113:1151	AMPs conjugated marine oligosaccharides	1113:1151	Our data suggest that AMPs conjugated marine oligosaccharides and AgNPs may be effective and safe antibacterial materials for wound therapy.
34294293	6	3	contain	had	1014:1016	arg1	AGO-AgNPs-OA					939:950	AGO-AgNPs-OA	939:950	AGO-AgNPs-OA	939:950	AGO-AgNPs-OA showed stronger anti-bacterial activities than AGO-AgNPs, and had good biocompatibility and significant promoting effect on wound healing.
34294293	6	3	contain	had	1014:1016	arg2	biocompatibility					1023:1038	good biocompatibility	1018:1038	good biocompatibility	1018:1038	AGO-AgNPs-OA showed stronger anti-bacterial activities than AGO-AgNPs, and had good biocompatibility and significant promoting effect on wound healing.
34294293	6	3	contain	had	1014:1016	arg2	effect					1066:1071	significant promoting effect	1044:1071	significant promoting effect	1044:1071	AGO-AgNPs-OA showed stronger anti-bacterial activities than AGO-AgNPs, and had good biocompatibility and significant promoting effect on wound healing.
34294293	5	4	theme	OA	935:936	arg1	structure					922:930	the α-helical structure	908:930	the α-helical structure of OA	908:936	Circular dichroism (CD) spectroscopy showed that AGO-AgNPs stabilized the α-helical structure of OA.
34294293	4	5	with	spherical	781:789	arg1	size					816:819	average size	808:819	average size of about 100 nm	808:835	Transmission electron microscopy (TEM) and Malvern particle size analyzer showed that AGO-AgNPs-OA was spherical or elliptic with average size of about 100 nm.
34294293	7	6	theme	wound	1217:1221	arg1	therapy					1223:1229	wound therapy	1217:1229	wound therapy	1217:1229	Our data suggest that AMPs conjugated marine oligosaccharides and AgNPs may be effective and safe antibacterial materials for wound therapy.
34294293	3	7	dep	used	437:440	arg1	connected					549:557	connected	549:557	connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA)	549:675	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	2	8	theme	wound	327:331	arg1	dressings					333:341	novel antimicrobial wound dressings	307:341	novel antimicrobial wound dressings	307:341	However, microbial infection delays wound healing process, and novel antimicrobial wound dressings are urgently needed.
34294293	7	9	theme	AMPs	1113:1116	arg1	oligosaccharides					1136:1151	AMPs conjugated marine oligosaccharides	1113:1151	AMPs conjugated marine oligosaccharides	1113:1151	Our data suggest that AMPs conjugated marine oligosaccharides and AgNPs may be effective and safe antibacterial materials for wound therapy.
34294293	4	10	theme	100 nm	830:835	arg1	size					816:819	average size	808:819	average size of about 100 nm	808:835	Transmission electron microscopy (TEM) and Malvern particle size analyzer showed that AGO-AgNPs-OA was spherical or elliptic with average size of about 100 nm.
34294293	1	11	theme	Marine	99:104	arg1	polysaccharides					106:120	Marine polysaccharides	99:120	Marine polysaccharides	99:120	Marine polysaccharides or oligosaccharides have potential to promote wound healing due to their biocompatibility and physicochemical properties.
34294293	3	12	theme	green	475:479	arg1	synthesis					481:489	green synthesis	475:489	green synthesis of silver nanoparticles (AgNPs)	475:521	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	0	13	theme	oligosaccharide-	8:23	arg1	peptide-					60:67	Agarose oligosaccharide- silver nanoparticle- antimicrobial peptide-	0:67	Agarose oligosaccharide- silver nanoparticle- antimicrobial peptide-	0:67	Agarose oligosaccharide- silver nanoparticle- antimicrobial peptide- composite for wound dressing.
34294293	4	14	theme	particle	729:736	arg1	analyzer					743:750	Malvern particle size analyzer	721:750	Malvern particle size analyzer	721:750	Transmission electron microscopy (TEM) and Malvern particle size analyzer showed that AGO-AgNPs-OA was spherical or elliptic with average size of about 100 nm.
34294293	1	15	theme	physicochemical	216:230	arg1	properties					232:241	physicochemical properties	216:241	physicochemical properties	216:241	Marine polysaccharides or oligosaccharides have potential to promote wound healing due to their biocompatibility and physicochemical properties.
34294293	0	16	theme	Agarose	0:6	arg1	peptide-					60:67	Agarose oligosaccharide- silver nanoparticle- antimicrobial peptide-	0:67	Agarose oligosaccharide- silver nanoparticle- antimicrobial peptide-	0:67	Agarose oligosaccharide- silver nanoparticle- antimicrobial peptide- composite for wound dressing.
34294293	0	17	theme	nanoparticle-	32:44	arg1	peptide-					60:67	Agarose oligosaccharide- silver nanoparticle- antimicrobial peptide-	0:67	Agarose oligosaccharide- silver nanoparticle- antimicrobial peptide-	0:67	Agarose oligosaccharide- silver nanoparticle- antimicrobial peptide- composite for wound dressing.
34294293	4	18	theme	Malvern	721:727	arg1	analyzer					743:750	Malvern particle size analyzer	721:750	Malvern particle size analyzer	721:750	Transmission electron microscopy (TEM) and Malvern particle size analyzer showed that AGO-AgNPs-OA was spherical or elliptic with average size of about 100 nm.
34294293	3	19	theme	marine	415:420	arg1	algae					426:430	marine red algae	415:430	marine red algae	415:430	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	2	20	theme	healing	286:292	arg1	process					294:300	wound healing process	280:300	wound healing process	280:300	However, microbial infection delays wound healing process, and novel antimicrobial wound dressings are urgently needed.
34294293	3	21	theme	silver	494:499	arg1	AgNPs					516:520	AgNPs	516:520	AgNPs	516:520	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	3	21	theme	silver	494:499	arg1	nanoparticles					501:513	silver nanoparticles	494:513	silver nanoparticles (AgNPs)	494:521	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	0	22	theme	silver	25:30	arg1	peptide-					60:67	Agarose oligosaccharide- silver nanoparticle- antimicrobial peptide-	0:67	Agarose oligosaccharide- silver nanoparticle- antimicrobial peptide-	0:67	Agarose oligosaccharide- silver nanoparticle- antimicrobial peptide- composite for wound dressing.
34294293	6	23	theme	anti-bacterial	968:981	arg1	activities					983:992	stronger anti-bacterial activities	959:992	stronger anti-bacterial activities	959:992	AGO-AgNPs-OA showed stronger anti-bacterial activities than AGO-AgNPs, and had good biocompatibility and significant promoting effect on wound healing.
34294293	3	24	theme	novel	633:637	arg1	AGO-AgNPs-OA					663:674	AGO-AgNPs-OA	663:674	AGO-AgNPs-OA	663:674	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	3	24	theme	novel	633:637	arg1	nanomaterial					649:660	a novel composite nanomaterial	631:660	a novel composite nanomaterial (AGO-AgNPs-OA)	631:675	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	4	25	theme	size	738:741	arg1	analyzer					743:750	Malvern particle size analyzer	721:750	Malvern particle size analyzer	721:750	Transmission electron microscopy (TEM) and Malvern particle size analyzer showed that AGO-AgNPs-OA was spherical or elliptic with average size of about 100 nm.
34294293	3	26	theme	nanoparticles	501:513	arg1	synthesis					481:489	green synthesis	475:489	green synthesis of silver nanoparticles (AgNPs)	475:521	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	7	27	theme	effective	1170:1178	arg1	materials					1203:1211	effective and safe antibacterial materials	1170:1211	effective and safe antibacterial materials	1170:1211	Our data suggest that AMPs conjugated marine oligosaccharides and AgNPs may be effective and safe antibacterial materials for wound therapy.
34294293	6	28	theme	stronger	959:966	arg1	activities					983:992	stronger anti-bacterial activities	959:992	stronger anti-bacterial activities	959:992	AGO-AgNPs-OA showed stronger anti-bacterial activities than AGO-AgNPs, and had good biocompatibility and significant promoting effect on wound healing.
34294293	3	29	dep	oligosaccharides	378:393	arg1	AGO					396:398	AGO	396:398	AGO	396:398	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	3	30	theme	composite	639:647	arg1	AGO-AgNPs-OA					663:674	AGO-AgNPs-OA	663:674	AGO-AgNPs-OA	663:674	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	3	30	theme	composite	639:647	arg1	nanomaterial					649:660	a novel composite nanomaterial	631:660	a novel composite nanomaterial (AGO-AgNPs-OA)	631:675	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	3	31	theme	peptides	604:611	arg1	peptides					604:611	antimicrobial peptides	590:611	antimicrobial peptides (AMPs)	590:618	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	3	31	theme	peptides	604:611	arg1	A					575:575	odorranain A	564:575	odorranain A (OA)	564:580	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	3	31	theme	peptides	604:611	arg1	AMPs					614:617	AMPs	614:617	AMPs	614:617	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	3	31	theme	peptides	604:611	arg1	one					583:585	one	583:585	one	583:585	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	5	32	theme	Circular	838:845	arg1	CD					858:859	CD	858:859	CD	858:859	Circular dichroism (CD) spectroscopy showed that AGO-AgNPs stabilized the α-helical structure of OA.
34294293	5	32	theme	Circular	838:845	arg1	dichroism					847:855	Circular dichroism	838:855	Circular dichroism (CD) spectroscopy	838:873	Circular dichroism (CD) spectroscopy showed that AGO-AgNPs stabilized the α-helical structure of OA.
34294293	0	33	theme	antimicrobial	46:58	arg1	peptide-					60:67	Agarose oligosaccharide- silver nanoparticle- antimicrobial peptide-	0:67	Agarose oligosaccharide- silver nanoparticle- antimicrobial peptide-	0:67	Agarose oligosaccharide- silver nanoparticle- antimicrobial peptide- composite for wound dressing.
34294293	1	34	contain	have	142:145	arg2	potential					147:155	potential	147:155	potential to promote wound healing due to their biocompatibility and physicochemical properties	147:241	Marine polysaccharides or oligosaccharides have potential to promote wound healing due to their biocompatibility and physicochemical properties.
34294293	1	34	contain	have	142:145	arg1	oligosaccharides					125:140	oligosaccharides	125:140	oligosaccharides	125:140	Marine polysaccharides or oligosaccharides have potential to promote wound healing due to their biocompatibility and physicochemical properties.
34294293	1	34	contain	have	142:145	arg1	polysaccharides					106:120	Marine polysaccharides	99:120	Marine polysaccharides	99:120	Marine polysaccharides or oligosaccharides have potential to promote wound healing due to their biocompatibility and physicochemical properties.
34294293	2	35	theme	wound	280:284	arg1	healing					286:292	wound healing	280:292	wound healing process	280:300	However, microbial infection delays wound healing process, and novel antimicrobial wound dressings are urgently needed.
34294293	5	36	theme	dichroism	847:855	arg1	spectroscopy					862:873	Circular dichroism (CD) spectroscopy	838:873	Circular dichroism (CD) spectroscopy	838:873	Circular dichroism (CD) spectroscopy showed that AGO-AgNPs stabilized the α-helical structure of OA.
34294293	0	37	theme	wound	83:87	arg1	dressing					89:96	wound dressing	83:96	wound dressing	83:96	Agarose oligosaccharide- silver nanoparticle- antimicrobial peptide- composite for wound dressing.
34294293	3	38	used	used	437:440	arg2	a					445:445	a	445:445	a	445:445	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	3	38	used	used	437:440	arg2	stabilizer					460:469	stabilizer	460:469	stabilizer	460:469	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	3	38	used	used	437:440	arg2	oligosaccharides					378:393	agarose oligosaccharides	370:393	agarose oligosaccharides (AGO) obtained from marine red algae	370:430	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	2	39	theme	microbial	253:261	arg1	infection					263:271	microbial infection	253:271	microbial infection	253:271	However, microbial infection delays wound healing process, and novel antimicrobial wound dressings are urgently needed.
34294293	3	40	theme	antimicrobial	590:602	arg1	peptides					604:611	antimicrobial peptides	590:611	antimicrobial peptides (AMPs)	590:618	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	3	40	theme	antimicrobial	590:602	arg1	AMPs					614:617	AMPs	614:617	AMPs	614:617	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	5	41	theme	α-helical	912:920	arg1	structure					922:930	the α-helical structure	908:930	the α-helical structure of OA	908:936	Circular dichroism (CD) spectroscopy showed that AGO-AgNPs stabilized the α-helical structure of OA.
34294293	7	42	theme	safe	1184:1187	arg1	materials					1203:1211	effective and safe antibacterial materials	1170:1211	effective and safe antibacterial materials	1170:1211	Our data suggest that AMPs conjugated marine oligosaccharides and AgNPs may be effective and safe antibacterial materials for wound therapy.
34294293	2	43	theme	novel	307:311	arg1	dressings					333:341	novel antimicrobial wound dressings	307:341	novel antimicrobial wound dressings	307:341	However, microbial infection delays wound healing process, and novel antimicrobial wound dressings are urgently needed.
34294293	4	44	theme	average	808:814	arg1	size					816:819	average size	808:819	average size of about 100 nm	808:835	Transmission electron microscopy (TEM) and Malvern particle size analyzer showed that AGO-AgNPs-OA was spherical or elliptic with average size of about 100 nm.
34294293	6	45	theme	significant	1044:1054	arg1	effect					1066:1071	significant promoting effect	1044:1071	significant promoting effect	1044:1071	AGO-AgNPs-OA showed stronger anti-bacterial activities than AGO-AgNPs, and had good biocompatibility and significant promoting effect on wound healing.
34294293	3	46	theme	odorranain	564:573	arg1	A					575:575	odorranain A	564:575	odorranain A (OA)	564:580	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	3	46	theme	odorranain	564:573	arg1	peptides					604:611	antimicrobial peptides	590:611	antimicrobial peptides (AMPs)	590:618	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	3	46	theme	odorranain	564:573	arg1	OA					578:579	OA	578:579	OA	578:579	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	3	46	theme	odorranain	564:573	arg1	one					583:585	one	583:585	one	583:585	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	7	47	theme	conjugated	1118:1127	arg1	oligosaccharides					1136:1151	AMPs conjugated marine oligosaccharides	1113:1151	AMPs conjugated marine oligosaccharides	1113:1151	Our data suggest that AMPs conjugated marine oligosaccharides and AgNPs may be effective and safe antibacterial materials for wound therapy.
34294293	1	48	theme	wound	168:172	arg1	healing					174:180	wound healing	168:180	wound healing due to their biocompatibility and physicochemical properties	168:241	Marine polysaccharides or oligosaccharides have potential to promote wound healing due to their biocompatibility and physicochemical properties.
34294293	6	49	theme	promoting	1056:1064	arg1	effect					1066:1071	significant promoting effect	1044:1071	significant promoting effect	1044:1071	AGO-AgNPs-OA showed stronger anti-bacterial activities than AGO-AgNPs, and had good biocompatibility and significant promoting effect on wound healing.
34294293	6	50	theme	wound	1076:1080	arg1	healing					1082:1088	wound healing	1076:1088	wound healing	1076:1088	AGO-AgNPs-OA showed stronger anti-bacterial activities than AGO-AgNPs, and had good biocompatibility and significant promoting effect on wound healing.
34294293	4	51	theme	Transmission	678:689	arg1	TEM					712:714	TEM	712:714	TEM	712:714	Transmission electron microscopy (TEM) and Malvern particle size analyzer showed that AGO-AgNPs-OA was spherical or elliptic with average size of about 100 nm.
34294293	4	51	theme	Transmission	678:689	arg1	microscopy					700:709	Transmission electron microscopy	678:709	Transmission electron microscopy (TEM)	678:715	Transmission electron microscopy (TEM) and Malvern particle size analyzer showed that AGO-AgNPs-OA was spherical or elliptic with average size of about 100 nm.
34294293	2	52	theme	antimicrobial	313:325	arg1	dressings					333:341	novel antimicrobial wound dressings	307:341	novel antimicrobial wound dressings	307:341	However, microbial infection delays wound healing process, and novel antimicrobial wound dressings are urgently needed.
34294293	3	53	theme	red	422:424	arg1	algae					426:430	marine red algae	415:430	marine red algae	415:430	Here, agarose oligosaccharides (AGO) obtained from marine red algae were used as a reducing and stabilizer for green synthesis of silver nanoparticles (AgNPs), and further successfully connected with odorranain A (OA), one of antimicrobial peptides (AMPs), to obtain a novel composite nanomaterial (AGO-AgNPs-OA).
34294293	7	54	theme	antibacterial	1189:1201	arg1	materials					1203:1211	effective and safe antibacterial materials	1170:1211	effective and safe antibacterial materials	1170:1211	Our data suggest that AMPs conjugated marine oligosaccharides and AgNPs may be effective and safe antibacterial materials for wound therapy.
34294293	6	55	theme	good	1018:1021	arg1	biocompatibility					1023:1038	good biocompatibility	1018:1038	good biocompatibility	1018:1038	AGO-AgNPs-OA showed stronger anti-bacterial activities than AGO-AgNPs, and had good biocompatibility and significant promoting effect on wound healing.
32475601	7	0	theme	hepatic	956:962	arg1	tissue					964:969	hepatic tissue	956:969	hepatic tissue	956:969	The lipid droplet accumulation in hepatic tissue was similar between lean and LCPS groups lower than in HFD-induced mice from tissue section staining results.
32475601	4	1	theme	magnetic	607:614	arg1	resonance					616:624	nuclear magnetic resonance	599:624	nuclear magnetic resonance (NMR) spectroscopy	599:643	Chemical structure of LCPS was indicated by nuclear magnetic resonance (NMR) spectroscopy.
32475601	4	1	theme	magnetic	607:614	arg1	NMR					627:629	NMR	627:629	NMR	627:629	Chemical structure of LCPS was indicated by nuclear magnetic resonance (NMR) spectroscopy.
32475601	5	2	theme	-β-D-Fruf-	718:727	arg1	linkage					734:740	α-D-Glcp-(1→[1)-β-D-Fruf-(2]15 linkage	703:740	α-D-Glcp-(1→[1)-β-D-Fruf-(2]15 linkage	703:740	Data indicated that LCPS was an inulin-type fructan with α-D-Glcp-(1→[1)-β-D-Fruf-(2]15 linkage.
32475601	7	3	theme	droplet	932:938	arg1	similar					975:981	similar	975:981	similar	975:981	The lipid droplet accumulation in hepatic tissue was similar between lean and LCPS groups lower than in HFD-induced mice from tissue section staining results.
32475601	7	3	theme	droplet	932:938	arg1	accumulation					940:951	The lipid droplet accumulation	922:951	The lipid droplet accumulation in hepatic tissue	922:969	The lipid droplet accumulation in hepatic tissue was similar between lean and LCPS groups lower than in HFD-induced mice from tissue section staining results.
32475601	3	4	theme	GC-MS	540:544	arg1	method					547:552	gas chromatography-mass spectrometer (GC-MS) method	502:552	gas chromatography-mass spectrometer (GC-MS) method	502:552	The chemical composition of the extracted polysaccharide contains fructose and glucose with protein-free identified by gas chromatography-mass spectrometer (GC-MS) method.
32475601	4	5	theme	nuclear	599:605	arg1	resonance					616:624	nuclear magnetic resonance	599:624	nuclear magnetic resonance (NMR) spectroscopy	599:643	Chemical structure of LCPS was indicated by nuclear magnetic resonance (NMR) spectroscopy.
32475601	4	5	theme	nuclear	599:605	arg1	NMR					627:629	NMR	627:629	NMR	627:629	Chemical structure of LCPS was indicated by nuclear magnetic resonance (NMR) spectroscopy.
32475601	0	6	theme	anti-obesity	99:110	arg1	activity					112:119	anti-obesity activity	99:119	anti-obesity activity	99:119	Chemical structure elucidation of an inulin-type fructan isolated from Lobelia chinensis lour with anti-obesity activity on diet-induced mice.
32475601	5	7	theme	inulin-type	678:688	arg1	LCPS					666:669	LCPS	666:669	LCPS	666:669	Data indicated that LCPS was an inulin-type fructan with α-D-Glcp-(1→[1)-β-D-Fruf-(2]15 linkage.
32475601	5	7	theme	inulin-type	678:688	arg1	fructan					690:696	an inulin-type fructan	675:696	an inulin-type fructan with α-D-Glcp-(1→[1)-β-D-Fruf-(2]15 linkage	675:740	Data indicated that LCPS was an inulin-type fructan with α-D-Glcp-(1→[1)-β-D-Fruf-(2]15 linkage.
32475601	4	8	theme	resonance	616:624	arg1	spectroscopy					632:643	nuclear magnetic resonance (NMR) spectroscopy	599:643	nuclear magnetic resonance (NMR) spectroscopy	599:643	Chemical structure of LCPS was indicated by nuclear magnetic resonance (NMR) spectroscopy.
32475601	6	9	from	triglyceride	866:877	arg1	serum					882:886	serum	882:886	serum	882:886	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	6	9	from	triglyceride	866:877	arg1	tissue					900:905	hepatic tissue	892:905	hepatic tissue	892:905	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	7	10	theme	staining	1063:1070	arg1	results					1072:1078	tissue section staining results	1048:1078	tissue section staining results	1048:1078	The lipid droplet accumulation in hepatic tissue was similar between lean and LCPS groups lower than in HFD-induced mice from tissue section staining results.
32475601	7	11	theme	lipid	926:930	arg1	similar					975:981	similar	975:981	similar	975:981	The lipid droplet accumulation in hepatic tissue was similar between lean and LCPS groups lower than in HFD-induced mice from tissue section staining results.
32475601	7	11	theme	lipid	926:930	arg1	accumulation					940:951	The lipid droplet accumulation	922:951	The lipid droplet accumulation in hepatic tissue	922:969	The lipid droplet accumulation in hepatic tissue was similar between lean and LCPS groups lower than in HFD-induced mice from tissue section staining results.
32475601	8	12	theme	manufacturing	1226:1238	arg1	supplements					1240:1250	the manufacturing supplements	1222:1250	the manufacturing supplements	1222:1250	Together, polysaccharides from Lobelia chinensis could be a new source of natural anti-obesity agent against obesity with potential value in the manufacturing supplements and drugs.
32475601	8	13	theme	new	1141:1143	arg1	polysaccharides					1091:1105	polysaccharides	1091:1105	polysaccharides from Lobelia chinensis	1091:1128	Together, polysaccharides from Lobelia chinensis could be a new source of natural anti-obesity agent against obesity with potential value in the manufacturing supplements and drugs.
32475601	8	13	theme	new	1141:1143	arg1	source					1145:1150	a new source	1139:1150	a new source of natural anti-obesity agent against obesity with potential value in the manufacturing supplements and drugs	1139:1260	Together, polysaccharides from Lobelia chinensis could be a new source of natural anti-obesity agent against obesity with potential value in the manufacturing supplements and drugs.
32475601	6	14	theme	fat	789:791	arg1	diet					793:796	high fat diet	784:796	high fat diet	784:796	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	3	15	theme	chemical	387:394	arg1	composition					396:406	The chemical composition	383:406	The chemical composition of the extracted polysaccharide	383:438	The chemical composition of the extracted polysaccharide contains fructose and glucose with protein-free identified by gas chromatography-mass spectrometer (GC-MS) method.
32475601	0	16	theme	structure	9:17	arg1	elucidation					19:29	Chemical structure elucidation	0:29	Chemical structure elucidation of an inulin-type fructan isolated from Lobelia chinensis	0:87	Chemical structure elucidation of an inulin-type fructan isolated from Lobelia chinensis lour with anti-obesity activity on diet-induced mice.
32475601	6	17	theme	total	844:848	arg1	gain					824:827	high fat diet (HFD)-induced body weight gain	784:827	high fat diet (HFD)-induced body weight gain	784:827	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	6	17	theme	total	844:848	arg1	cholesterol					850:860	total cholesterol	844:860	total cholesterol	844:860	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	7	18	theme	tissue	1048:1053	arg1	results					1072:1078	tissue section staining results	1048:1078	tissue section staining results	1048:1078	The lipid droplet accumulation in hepatic tissue was similar between lean and LCPS groups lower than in HFD-induced mice from tissue section staining results.
32475601	6	19	theme	high	784:787	arg1	diet					793:796	high fat diet	784:796	high fat diet	784:796	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	3	20	theme	polysaccharide	425:438	arg1	composition					396:406	The chemical composition	383:406	The chemical composition of the extracted polysaccharide	383:438	The chemical composition of the extracted polysaccharide contains fructose and glucose with protein-free identified by gas chromatography-mass spectrometer (GC-MS) method.
32475601	0	21	theme	Chemical	0:7	arg1	elucidation					19:29	Chemical structure elucidation	0:29	Chemical structure elucidation of an inulin-type fructan isolated from Lobelia chinensis	0:87	Chemical structure elucidation of an inulin-type fructan isolated from Lobelia chinensis lour with anti-obesity activity on diet-induced mice.
32475601	7	22	theme	section	1055:1061	arg1	results					1072:1078	tissue section staining results	1048:1078	tissue section staining results	1048:1078	The lipid droplet accumulation in hepatic tissue was similar between lean and LCPS groups lower than in HFD-induced mice from tissue section staining results.
32475601	2	23	theme	Da	379:380	arg1	weight					359:364	a molecular weight	347:364	a molecular weight of 2.6 × 103 Da	347:380	After separation and purification, the homogeneous polysaccharide was obtained with a molecular weight of 2.6 × 103 Da.
32475601	8	24	theme	agent	1176:1180	arg1	polysaccharides					1091:1105	polysaccharides	1091:1105	polysaccharides from Lobelia chinensis	1091:1128	Together, polysaccharides from Lobelia chinensis could be a new source of natural anti-obesity agent against obesity with potential value in the manufacturing supplements and drugs.
32475601	8	24	theme	agent	1176:1180	arg1	source					1145:1150	a new source	1139:1150	a new source of natural anti-obesity agent against obesity with potential value in the manufacturing supplements and drugs	1139:1260	Together, polysaccharides from Lobelia chinensis could be a new source of natural anti-obesity agent against obesity with potential value in the manufacturing supplements and drugs.
32475601	4	25	theme	LCPS	577:580	arg1	structure					564:572	Chemical structure	555:572	Chemical structure of LCPS	555:580	Chemical structure of LCPS was indicated by nuclear magnetic resonance (NMR) spectroscopy.
32475601	3	26	with	glucose	462:468	arg1	protein-free					475:486	protein-free	475:486	protein-free	475:486	The chemical composition of the extracted polysaccharide contains fructose and glucose with protein-free identified by gas chromatography-mass spectrometer (GC-MS) method.
32475601	0	27	attach	isolated	57:64	arg1	chinensis					79:87	chinensis	79:87	chinensis	79:87	Chemical structure elucidation of an inulin-type fructan isolated from Lobelia chinensis lour with anti-obesity activity on diet-induced mice.
32475601	0	27	attach	isolated	57:64	arg2	fructan					49:55	an inulin-type fructan	34:55	an inulin-type fructan isolated from Lobelia chinensis	34:87	Chemical structure elucidation of an inulin-type fructan isolated from Lobelia chinensis lour with anti-obesity activity on diet-induced mice.
32475601	8	28	theme	anti-obesity	1163:1174	arg1	agent					1176:1180	natural anti-obesity agent	1155:1180	natural anti-obesity agent against obesity with potential value in the manufacturing supplements and drugs	1155:1260	Together, polysaccharides from Lobelia chinensis could be a new source of natural anti-obesity agent against obesity with potential value in the manufacturing supplements and drugs.
32475601	0	29	theme	diet-induced	124:135	arg1	mice					137:140	diet-induced mice	124:140	diet-induced mice	124:140	Chemical structure elucidation of an inulin-type fructan isolated from Lobelia chinensis lour with anti-obesity activity on diet-induced mice.
32475601	2	30	theme	homogeneous	302:312	arg1	polysaccharide					314:327	the homogeneous polysaccharide	298:327	the homogeneous polysaccharide	298:327	After separation and purification, the homogeneous polysaccharide was obtained with a molecular weight of 2.6 × 103 Da.
32475601	0	31	theme	inulin-type	37:47	arg1	fructan					49:55	an inulin-type fructan	34:55	an inulin-type fructan isolated from Lobelia chinensis	34:87	Chemical structure elucidation of an inulin-type fructan isolated from Lobelia chinensis lour with anti-obesity activity on diet-induced mice.
32475601	2	32	theme	molecular	349:357	arg1	weight					359:364	a molecular weight	347:364	a molecular weight of 2.6 × 103 Da	347:380	After separation and purification, the homogeneous polysaccharide was obtained with a molecular weight of 2.6 × 103 Da.
32475601	8	33	from	value	1213:1217	arg1	drugs					1256:1260	drugs	1256:1260	drugs	1256:1260	Together, polysaccharides from Lobelia chinensis could be a new source of natural anti-obesity agent against obesity with potential value in the manufacturing supplements and drugs.
32475601	8	33	from	value	1213:1217	arg1	supplements					1240:1250	the manufacturing supplements	1222:1250	the manufacturing supplements	1222:1250	Together, polysaccharides from Lobelia chinensis could be a new source of natural anti-obesity agent against obesity with potential value in the manufacturing supplements and drugs.
32475601	8	34	from	chinensis	1120:1128	arg1	source					1145:1150	a new source	1139:1150	a new source of natural anti-obesity agent against obesity with potential value in the manufacturing supplements and drugs	1139:1260	Together, polysaccharides from Lobelia chinensis could be a new source of natural anti-obesity agent against obesity with potential value in the manufacturing supplements and drugs.
32475601	8	34	from	chinensis	1120:1128	arg1	polysaccharides					1091:1105	polysaccharides	1091:1105	polysaccharides from Lobelia chinensis	1091:1128	Together, polysaccharides from Lobelia chinensis could be a new source of natural anti-obesity agent against obesity with potential value in the manufacturing supplements and drugs.
32475601	8	35	theme	natural	1155:1161	arg1	agent					1176:1180	natural anti-obesity agent	1155:1180	natural anti-obesity agent against obesity with potential value in the manufacturing supplements and drugs	1155:1260	Together, polysaccharides from Lobelia chinensis could be a new source of natural anti-obesity agent against obesity with potential value in the manufacturing supplements and drugs.
32475601	7	36	from	accumulation	940:951	arg1	tissue					964:969	hepatic tissue	956:969	hepatic tissue	956:969	The lipid droplet accumulation in hepatic tissue was similar between lean and LCPS groups lower than in HFD-induced mice from tissue section staining results.
32475601	3	37	theme	extracted	415:423	arg1	polysaccharide					425:438	the extracted polysaccharide	411:438	the extracted polysaccharide	411:438	The chemical composition of the extracted polysaccharide contains fructose and glucose with protein-free identified by gas chromatography-mass spectrometer (GC-MS) method.
32475601	4	38	theme	Chemical	555:562	arg1	structure					564:572	Chemical structure	555:572	Chemical structure of LCPS	555:580	Chemical structure of LCPS was indicated by nuclear magnetic resonance (NMR) spectroscopy.
32475601	7	39	theme	HFD-induced	1026:1036	arg1	mice					1038:1041	HFD-induced mice	1026:1041	HFD-induced mice from tissue section staining results	1026:1078	The lipid droplet accumulation in hepatic tissue was similar between lean and LCPS groups lower than in HFD-induced mice from tissue section staining results.
32475601	6	40	theme	liver	830:834	arg1	gain					824:827	high fat diet (HFD)-induced body weight gain	784:827	high fat diet (HFD)-induced body weight gain	784:827	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	6	40	theme	liver	830:834	arg1	weight					836:841	liver weight	830:841	liver weight	830:841	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	7	41	theme	lean	991:994	arg1	groups					1005:1010	lean and LCPS groups	991:1010	lean and LCPS groups lower than in HFD-induced mice from tissue section staining results	991:1078	The lipid droplet accumulation in hepatic tissue was similar between lean and LCPS groups lower than in HFD-induced mice from tissue section staining results.
32475601	1	42	theme	hot	215:217	arg1	extraction					225:234	hot water extraction	215:234	hot water extraction	215:234	A neutral polysaccharide (LCPS) was obtained from Lobelia chinensis via hot water extraction and ethanol precipitation.
32475601	8	43	theme	potential	1203:1211	arg1	value					1213:1217	potential value	1203:1217	potential value in the manufacturing supplements and drugs	1203:1260	Together, polysaccharides from Lobelia chinensis could be a new source of natural anti-obesity agent against obesity with potential value in the manufacturing supplements and drugs.
32475601	6	44	from	cholesterol	850:860	arg1	serum					882:886	serum	882:886	serum	882:886	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	6	44	from	cholesterol	850:860	arg1	tissue					900:905	hepatic tissue	892:905	hepatic tissue	892:905	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	7	45	theme	lower	1012:1016	arg1	groups					1005:1010	lean and LCPS groups	991:1010	lean and LCPS groups lower than in HFD-induced mice from tissue section staining results	991:1078	The lipid droplet accumulation in hepatic tissue was similar between lean and LCPS groups lower than in HFD-induced mice from tissue section staining results.
32475601	6	46	dep	-induced	803:810	arg1	diet					793:796	high fat diet	784:796	high fat diet	784:796	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	7	47	theme	LCPS	1000:1003	arg1	groups					1005:1010	lean and LCPS groups	991:1010	lean and LCPS groups lower than in HFD-induced mice from tissue section staining results	991:1078	The lipid droplet accumulation in hepatic tissue was similar between lean and LCPS groups lower than in HFD-induced mice from tissue section staining results.
32475601	3	48	theme	chromatography-mass	506:524	arg1	method					547:552	gas chromatography-mass spectrometer (GC-MS) method	502:552	gas chromatography-mass spectrometer (GC-MS) method	502:552	The chemical composition of the extracted polysaccharide contains fructose and glucose with protein-free identified by gas chromatography-mass spectrometer (GC-MS) method.
32475601	0	49	theme	fructan	49:55	arg1	elucidation					19:29	Chemical structure elucidation	0:29	Chemical structure elucidation of an inulin-type fructan isolated from Lobelia chinensis	0:87	Chemical structure elucidation of an inulin-type fructan isolated from Lobelia chinensis lour with anti-obesity activity on diet-induced mice.
32475601	5	50	theme	α-D-Glcp-	703:711	arg1	linkage					734:740	α-D-Glcp-(1→[1)-β-D-Fruf-(2]15 linkage	703:740	α-D-Glcp-(1→[1)-β-D-Fruf-(2]15 linkage	703:740	Data indicated that LCPS was an inulin-type fructan with α-D-Glcp-(1→[1)-β-D-Fruf-(2]15 linkage.
32475601	3	51	contain	contains	440:447	arg2	glucose					462:468	glucose	462:468	glucose	462:468	The chemical composition of the extracted polysaccharide contains fructose and glucose with protein-free identified by gas chromatography-mass spectrometer (GC-MS) method.
32475601	3	51	contain	contains	440:447	arg1	composition					396:406	The chemical composition	383:406	The chemical composition of the extracted polysaccharide	383:438	The chemical composition of the extracted polysaccharide contains fructose and glucose with protein-free identified by gas chromatography-mass spectrometer (GC-MS) method.
32475601	3	51	contain	contains	440:447	arg2	fructose					449:456	fructose	449:456	fructose	449:456	The chemical composition of the extracted polysaccharide contains fructose and glucose with protein-free identified by gas chromatography-mass spectrometer (GC-MS) method.
32475601	3	52	theme	spectrometer	526:537	arg1	method					547:552	gas chromatography-mass spectrometer (GC-MS) method	502:552	gas chromatography-mass spectrometer (GC-MS) method	502:552	The chemical composition of the extracted polysaccharide contains fructose and glucose with protein-free identified by gas chromatography-mass spectrometer (GC-MS) method.
32475601	6	53	theme	significant	766:776	arg1	level					778:782	significant level	766:782	significant level	766:782	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	3	54	with	fructose	449:456	arg1	protein-free					475:486	protein-free	475:486	protein-free	475:486	The chemical composition of the extracted polysaccharide contains fructose and glucose with protein-free identified by gas chromatography-mass spectrometer (GC-MS) method.
32475601	6	55	theme	weight	817:822	arg1	triglyceride					866:877	triglyceride	866:877	triglyceride	866:877	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	6	55	theme	weight	817:822	arg1	gain					824:827	high fat diet (HFD)-induced body weight gain	784:827	high fat diet (HFD)-induced body weight gain	784:827	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	6	55	theme	weight	817:822	arg1	weight					836:841	liver weight	830:841	liver weight	830:841	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	6	55	theme	weight	817:822	arg1	cholesterol					850:860	total cholesterol	844:860	total cholesterol	844:860	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	6	56	theme	LCPS	743:746	arg1	intake					748:753	LCPS intake	743:753	LCPS intake	743:753	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	1	57	theme	water	219:223	arg1	extraction					225:234	hot water extraction	215:234	hot water extraction	215:234	A neutral polysaccharide (LCPS) was obtained from Lobelia chinensis via hot water extraction and ethanol precipitation.
32475601	5	58	theme	1→[1	713:716	arg1	linkage					734:740	α-D-Glcp-(1→[1)-β-D-Fruf-(2]15 linkage	703:740	α-D-Glcp-(1→[1)-β-D-Fruf-(2]15 linkage	703:740	Data indicated that LCPS was an inulin-type fructan with α-D-Glcp-(1→[1)-β-D-Fruf-(2]15 linkage.
32475601	6	59	theme	body	812:815	arg1	triglyceride					866:877	triglyceride	866:877	triglyceride	866:877	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	6	59	theme	body	812:815	arg1	gain					824:827	high fat diet (HFD)-induced body weight gain	784:827	high fat diet (HFD)-induced body weight gain	784:827	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	6	59	theme	body	812:815	arg1	weight					836:841	liver weight	830:841	liver weight	830:841	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	6	59	theme	body	812:815	arg1	cholesterol					850:860	total cholesterol	844:860	total cholesterol	844:860	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	7	60	from	results	1072:1078	arg1	mice					1038:1041	HFD-induced mice	1026:1041	HFD-induced mice from tissue section staining results	1026:1078	The lipid droplet accumulation in hepatic tissue was similar between lean and LCPS groups lower than in HFD-induced mice from tissue section staining results.
32475601	3	61	theme	gas	502:504	arg1	method					547:552	gas chromatography-mass spectrometer (GC-MS) method	502:552	gas chromatography-mass spectrometer (GC-MS) method	502:552	The chemical composition of the extracted polysaccharide contains fructose and glucose with protein-free identified by gas chromatography-mass spectrometer (GC-MS) method.
32475601	6	62	theme	-induced	803:810	arg1	triglyceride					866:877	triglyceride	866:877	triglyceride	866:877	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	6	62	theme	-induced	803:810	arg1	gain					824:827	high fat diet (HFD)-induced body weight gain	784:827	high fat diet (HFD)-induced body weight gain	784:827	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	6	62	theme	-induced	803:810	arg1	weight					836:841	liver weight	830:841	liver weight	830:841	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	6	62	theme	-induced	803:810	arg1	cholesterol					850:860	total cholesterol	844:860	total cholesterol	844:860	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	1	63	theme	neutral	145:151	arg1	polysaccharide					153:166	A neutral polysaccharide	143:166	A neutral polysaccharide (LCPS)	143:173	A neutral polysaccharide (LCPS) was obtained from Lobelia chinensis via hot water extraction and ethanol precipitation.
32475601	1	63	theme	neutral	145:151	arg1	LCPS					169:172	LCPS	169:172	LCPS	169:172	A neutral polysaccharide (LCPS) was obtained from Lobelia chinensis via hot water extraction and ethanol precipitation.
32475601	6	64	theme	hepatic	892:898	arg1	tissue					900:905	hepatic tissue	892:905	hepatic tissue	892:905	LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively.
32475601	8	65	with	obesity	1190:1196	arg1	value					1213:1217	potential value	1203:1217	potential value in the manufacturing supplements and drugs	1203:1260	Together, polysaccharides from Lobelia chinensis could be a new source of natural anti-obesity agent against obesity with potential value in the manufacturing supplements and drugs.
32475601	5	66	with	fructan	690:696	arg1	linkage					734:740	α-D-Glcp-(1→[1)-β-D-Fruf-(2]15 linkage	703:740	α-D-Glcp-(1→[1)-β-D-Fruf-(2]15 linkage	703:740	Data indicated that LCPS was an inulin-type fructan with α-D-Glcp-(1→[1)-β-D-Fruf-(2]15 linkage.
32475601	1	67	theme	ethanol	240:246	arg1	precipitation					248:260	ethanol precipitation	240:260	ethanol precipitation	240:260	A neutral polysaccharide (LCPS) was obtained from Lobelia chinensis via hot water extraction and ethanol precipitation.
32466624	4	0	theme	VEGF	573:576	arg1	inhibition					615:624	VEGF (vascular endothelial growth factor) inhibition	573:624	VEGF (vascular endothelial growth factor) inhibition	573:624	evanescens (FE) were tested for toxicity, oxidative stress protection and VEGF (vascular endothelial growth factor) inhibition.
32466624	5	1	theme	crude	642:646	arg1	fucoidans					648:656	crude fucoidans	642:656	crude fucoidans	642:656	For this study crude fucoidans were extracted from seaweeds using different enzymes and SL fucoidans were further separated into three fractions (SL_F1-F3) by ion-exchange chromatography (IEX).
32466624	4	2	theme	vascular	579:586	arg1	factor					607:612	vascular endothelial growth factor	579:612	vascular endothelial growth factor	579:612	evanescens (FE) were tested for toxicity, oxidative stress protection and VEGF (vascular endothelial growth factor) inhibition.
32466624	4	2	theme	vascular	579:586	arg1	VEGF					573:576	VEGF	573:576	VEGF (vascular endothelial growth factor) inhibition	573:624	evanescens (FE) were tested for toxicity, oxidative stress protection and VEGF (vascular endothelial growth factor) inhibition.
32466624	10	3	theme	immunosorbent	1350:1362	arg1	assay					1364:1368	enzyme-linked immunosorbent assay	1336:1368	enzyme-linked immunosorbent assay	1336:1368	Secreted VEGF was analyzed via ELISA (enzyme-linked immunosorbent assay) in ARPE-19 cells.
32466624	10	3	theme	immunosorbent	1350:1362	arg1	ELISA					1329:1333	ELISA	1329:1333	ELISA (enzyme-linked immunosorbent assay) in ARPE-19 cells	1329:1386	Secreted VEGF was analyzed via ELISA (enzyme-linked immunosorbent assay) in ARPE-19 cells.
32466624	13	4	theme	alginate	1577:1584	arg1	lyase					1586:1590	Sigma-Aldrich alginate lyase	1563:1590	Sigma-Aldrich alginate lyase	1563:1590	LD_SiAT2 (Cellic®CTec2 + Sigma-Aldrich alginate lyase), FE_SiAT3 (Cellic® CTec3 + Sigma-Aldrich alginate lyase), SL_F2 and SL_F3 inhibited VEGF with the latter two as the most effective.
32466624	6	5	theme	anion	875:879	arg1	chromatography					890:903	high performance anion exchange chromatography	858:903	high performance anion exchange chromatography (HPAEC)	858:911	Fucoidan composition was analyzed by high performance anion exchange chromatography (HPAEC) after acid hydrolysis.
32466624	6	5	theme	anion	875:879	arg1	HPAEC					906:910	HPAEC	906:910	HPAEC	906:910	Fucoidan composition was analyzed by high performance anion exchange chromatography (HPAEC) after acid hydrolysis.
32466624	10	6	link	enzyme-linked	1336:1348	arg1	assay					1364:1368	enzyme-linked immunosorbent assay	1336:1368	enzyme-linked immunosorbent assay	1336:1368	Secreted VEGF was analyzed via ELISA (enzyme-linked immunosorbent assay) in ARPE-19 cells.
32466624	10	6	link	enzyme-linked	1336:1348	arg1	ELISA					1329:1333	ELISA	1329:1333	ELISA (enzyme-linked immunosorbent assay) in ARPE-19 cells	1329:1386	Secreted VEGF was analyzed via ELISA (enzyme-linked immunosorbent assay) in ARPE-19 cells.
32466624	10	7	theme	ARPE-19	1374:1380	arg1	cells					1382:1386	ARPE-19 cells	1374:1386	ARPE-19 cells	1374:1386	Secreted VEGF was analyzed via ELISA (enzyme-linked immunosorbent assay) in ARPE-19 cells.
32466624	2	8	gly	heterogeneity	248:260	arg1	fucoidans					265:273	fucoidans	265:273	fucoidans	265:273	The heterogeneity of fucoidans requires intensive research in order to find suitable species and extraction methods.
32466624	13	9	theme	Cellic®	1547:1553	arg1	CTec3					1555:1559	Cellic® CTec3	1547:1559	Cellic® CTec3	1547:1559	LD_SiAT2 (Cellic®CTec2 + Sigma-Aldrich alginate lyase), FE_SiAT3 (Cellic® CTec3 + Sigma-Aldrich alginate lyase), SL_F2 and SL_F3 inhibited VEGF with the latter two as the most effective.
32466624	1	10	theme	age-related	204:214	arg1	AMD					238:240	AMD	238:240	AMD	238:240	Fucoidans from brown seaweeds are promising substances as potential drugs against age-related macular degeneration (AMD).
32466624	1	10	theme	age-related	204:214	arg1	degeneration					224:235	age-related macular degeneration	204:235	age-related macular degeneration (AMD)	204:241	Fucoidans from brown seaweeds are promising substances as potential drugs against age-related macular degeneration (AMD).
32466624	5	11	theme	SL	715:716	arg1	fucoidans					718:726	SL fucoidans	715:726	SL fucoidans	715:726	For this study crude fucoidans were extracted from seaweeds using different enzymes and SL fucoidans were further separated into three fractions (SL_F1-F3) by ion-exchange chromatography (IEX).
32466624	9	12	theme	Protective	1211:1220	arg1	effects					1222:1228	Protective effects	1211:1228	Protective effects	1211:1228	Protective effects were investigated after 24 h of stress insult in OMM-1 and ARPE-19.
32466624	13	13	theme	alginate	1520:1527	arg1	lyase					1529:1533	Sigma-Aldrich alginate lyase	1506:1533	Sigma-Aldrich alginate lyase	1506:1533	LD_SiAT2 (Cellic®CTec2 + Sigma-Aldrich alginate lyase), FE_SiAT3 (Cellic® CTec3 + Sigma-Aldrich alginate lyase), SL_F2 and SL_F3 inhibited VEGF with the latter two as the most effective.
32466624	7	14	theme	fucoidans	1008:1016	arg1	SL_F2					1018:1022	SL_F2	1018:1022	SL_F2	1018:1022	The crude extracts contained alginate, while two of the fractionated SL fucoidans SL_F2 and SL_F3 were highly pure.
32466624	7	14	theme	fucoidans	1008:1016	arg1	fucoidans					1008:1016	the fractionated SL fucoidans	988:1016	the fractionated SL fucoidans SL_F2 and SL_F3	988:1032	The crude extracts contained alginate, while two of the fractionated SL fucoidans SL_F2 and SL_F3 were highly pure.
32466624	7	14	theme	fucoidans	1008:1016	arg1	two					981:983	two	981:983	two	981:983	The crude extracts contained alginate, while two of the fractionated SL fucoidans SL_F2 and SL_F3 were highly pure.
32466624	7	14	theme	fucoidans	1008:1016	arg1	SL_F3					1028:1032	SL_F3	1028:1032	SL_F3	1028:1032	The crude extracts contained alginate, while two of the fractionated SL fucoidans SL_F2 and SL_F3 were highly pure.
32466624	14	15	theme	relevant	1811:1818	arg1	activities					1831:1840	beneficial AMD relevant biological activities	1796:1840	beneficial AMD relevant biological activities	1796:1840	We could show that enzyme treated fucoidans in general and the fractionated SL fucoidans SL_F2 and SL_F3 are very promising for beneficial AMD relevant biological activities.
32466624	9	16	from	h	1257:1257	arg1	OMM-1					1279:1283	OMM-1	1279:1283	OMM-1	1279:1283	Protective effects were investigated after 24 h of stress insult in OMM-1 and ARPE-19.
32466624	9	16	from	h	1257:1257	arg1	ARPE-19					1289:1295	ARPE-19	1289:1295	ARPE-19	1289:1295	Protective effects were investigated after 24 h of stress insult in OMM-1 and ARPE-19.
32466624	7	17	theme	fractionated	992:1003	arg1	fucoidans					1008:1016	the fractionated SL fucoidans	988:1016	the fractionated SL fucoidans SL_F2 and SL_F3	988:1032	The crude extracts contained alginate, while two of the fractionated SL fucoidans SL_F2 and SL_F3 were highly pure.
32466624	7	17	theme	fractionated	992:1003	arg1	SL_F3					1028:1032	SL_F3	1028:1032	SL_F3	1028:1032	The crude extracts contained alginate, while two of the fractionated SL fucoidans SL_F2 and SL_F3 were highly pure.
32466624	7	17	theme	fractionated	992:1003	arg1	SL_F2					1018:1022	SL_F2	1018:1022	SL_F2	1018:1022	The crude extracts contained alginate, while two of the fractionated SL fucoidans SL_F2 and SL_F3 were highly pure.
32466624	14	18	theme	beneficial	1796:1805	arg1	activities					1831:1840	beneficial AMD relevant biological activities	1796:1840	beneficial AMD relevant biological activities	1796:1840	We could show that enzyme treated fucoidans in general and the fractionated SL fucoidans SL_F2 and SL_F3 are very promising for beneficial AMD relevant biological activities.
32466624	1	19	theme	macular	216:222	arg1	AMD					238:240	AMD	238:240	AMD	238:240	Fucoidans from brown seaweeds are promising substances as potential drugs against age-related macular degeneration (AMD).
32466624	1	19	theme	macular	216:222	arg1	degeneration					224:235	age-related macular degeneration	204:235	age-related macular degeneration (AMD)	204:241	Fucoidans from brown seaweeds are promising substances as potential drugs against age-related macular degeneration (AMD).
32466624	0	20	theme	Biological	70:79	arg1	Activities					81:90	the Biological Activities	66:90	the Biological Activities of Fucoidans in Ocular Cells	66:119	Effects of a Newly Developed Enzyme-Assisted Extraction Method on the Biological Activities of Fucoidans in Ocular Cells.
32466624	9	21	theme	stress	1262:1267	arg1	insult					1269:1274	stress insult	1262:1274	stress insult	1262:1274	Protective effects were investigated after 24 h of stress insult in OMM-1 and ARPE-19.
32466624	1	22	theme	promising	156:164	arg1	Fucoidans					122:130	Fucoidans	122:130	Fucoidans from brown seaweeds	122:150	Fucoidans from brown seaweeds are promising substances as potential drugs against age-related macular degeneration (AMD).
32466624	1	22	theme	promising	156:164	arg1	substances					166:175	promising substances	156:175	promising substances as potential drugs against age-related macular degeneration (AMD)	156:241	Fucoidans from brown seaweeds are promising substances as potential drugs against age-related macular degeneration (AMD).
32466624	3	23	theme	different	365:373	arg1	samples					384:390	Ten different fucoidan samples	361:390	Ten different fucoidan samples	361:390	Ten different fucoidan samples extracted enzymatically from Laminaria digitata (LD), Saccharina latissima (SL) and Fucus distichus subsp.
32466624	0	24	from	Effects	0:6	arg1	Activities					81:90	the Biological Activities	66:90	the Biological Activities of Fucoidans in Ocular Cells	66:119	Effects of a Newly Developed Enzyme-Assisted Extraction Method on the Biological Activities of Fucoidans in Ocular Cells.
32466624	11	25	theme	toxic	1409:1413	arg1	effects					1415:1421	no toxic effects	1406:1421	no toxic effects	1406:1421	Fucoidans showed no toxic effects.
32466624	8	26	theme	Cell	1052:1055	arg1	viability					1057:1065	Cell viability	1052:1065	Cell viability	1052:1065	Cell viability was assessed with an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay in OMM-1 and ARPE-19.
32466624	14	27	from	fucoidans	1747:1755	arg1	general					1715:1721	general	1715:1721	general	1715:1721	We could show that enzyme treated fucoidans in general and the fractionated SL fucoidans SL_F2 and SL_F3 are very promising for beneficial AMD relevant biological activities.
32466624	14	28	theme	SL	1744:1745	arg1	fucoidans					1747:1755	the fractionated SL fucoidans	1727:1755	the fractionated SL fucoidans SL_F2 and SL_F3	1727:1771	We could show that enzyme treated fucoidans in general and the fractionated SL fucoidans SL_F2 and SL_F3 are very promising for beneficial AMD relevant biological activities.
32466624	14	28	theme	SL	1744:1745	arg1	SL_F3					1767:1771	SL_F3	1767:1771	SL_F3	1767:1771	We could show that enzyme treated fucoidans in general and the fractionated SL fucoidans SL_F2 and SL_F3 are very promising for beneficial AMD relevant biological activities.
32466624	14	28	theme	SL	1744:1745	arg1	promising					1782:1790	promising	1782:1790	promising	1782:1790	We could show that enzyme treated fucoidans in general and the fractionated SL fucoidans SL_F2 and SL_F3 are very promising for beneficial AMD relevant biological activities.
32466624	14	28	theme	SL	1744:1745	arg1	SL_F2					1757:1761	SL_F2	1757:1761	SL_F2	1757:1761	We could show that enzyme treated fucoidans in general and the fractionated SL fucoidans SL_F2 and SL_F3 are very promising for beneficial AMD relevant biological activities.
32466624	13	29	theme	Sigma-Aldrich	1563:1575	arg1	lyase					1586:1590	Sigma-Aldrich alginate lyase	1563:1590	Sigma-Aldrich alginate lyase	1563:1590	LD_SiAT2 (Cellic®CTec2 + Sigma-Aldrich alginate lyase), FE_SiAT3 (Cellic® CTec3 + Sigma-Aldrich alginate lyase), SL_F2 and SL_F3 inhibited VEGF with the latter two as the most effective.
32466624	6	30	theme	performance	863:873	arg1	chromatography					890:903	high performance anion exchange chromatography	858:903	high performance anion exchange chromatography (HPAEC)	858:911	Fucoidan composition was analyzed by high performance anion exchange chromatography (HPAEC) after acid hydrolysis.
32466624	6	30	theme	performance	863:873	arg1	HPAEC					906:910	HPAEC	906:910	HPAEC	906:910	Fucoidan composition was analyzed by high performance anion exchange chromatography (HPAEC) after acid hydrolysis.
32466624	4	31	theme	stress	551:556	arg1	protection					558:567	oxidative stress protection	541:567	oxidative stress protection	541:567	evanescens (FE) were tested for toxicity, oxidative stress protection and VEGF (vascular endothelial growth factor) inhibition.
32466624	0	32	theme	Developed	19:27	arg1	Method					56:61	a Newly Developed Enzyme-Assisted Extraction Method	11:61	a Newly Developed Enzyme-Assisted Extraction Method	11:61	Effects of a Newly Developed Enzyme-Assisted Extraction Method on the Biological Activities of Fucoidans in Ocular Cells.
32466624	10	33	from	ELISA	1329:1333	arg1	cells					1382:1386	ARPE-19 cells	1374:1386	ARPE-19 cells	1374:1386	Secreted VEGF was analyzed via ELISA (enzyme-linked immunosorbent assay) in ARPE-19 cells.
32466624	5	34	theme	ion-exchange	786:797	arg1	IEX					815:817	IEX	815:817	IEX	815:817	For this study crude fucoidans were extracted from seaweeds using different enzymes and SL fucoidans were further separated into three fractions (SL_F1-F3) by ion-exchange chromatography (IEX).
32466624	5	34	theme	ion-exchange	786:797	arg1	chromatography					799:812	ion-exchange chromatography	786:812	ion-exchange chromatography (IEX)	786:818	For this study crude fucoidans were extracted from seaweeds using different enzymes and SL fucoidans were further separated into three fractions (SL_F1-F3) by ion-exchange chromatography (IEX).
32466624	3	35	theme	Fucus	476:480	arg1	subsp					492:496	Fucus distichus subsp	476:496	Fucus distichus subsp	476:496	Ten different fucoidan samples extracted enzymatically from Laminaria digitata (LD), Saccharina latissima (SL) and Fucus distichus subsp.
32466624	8	36	theme	4,5-dimethylthiazol-2-yl	1091:1114	arg1	assay					1183:1187	an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay	1085:1187	an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay	1085:1187	Cell viability was assessed with an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay in OMM-1 and ARPE-19.
32466624	12	37	theme	FE	1451:1452	arg1	fucoidans					1454:1462	several FE fucoidans	1443:1462	several FE fucoidans	1443:1462	In OMM-1 SL_F2 and several FE fucoidans were protective.
32466624	4	38	theme	endothelial	588:598	arg1	factor					607:612	vascular endothelial growth factor	579:612	vascular endothelial growth factor	579:612	evanescens (FE) were tested for toxicity, oxidative stress protection and VEGF (vascular endothelial growth factor) inhibition.
32466624	4	38	theme	endothelial	588:598	arg1	VEGF					573:576	VEGF	573:576	VEGF (vascular endothelial growth factor) inhibition	573:624	evanescens (FE) were tested for toxicity, oxidative stress protection and VEGF (vascular endothelial growth factor) inhibition.
32466624	8	39	theme	-5-	1116:1118	arg1	assay					1183:1187	an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay	1085:1187	an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay	1085:1187	Cell viability was assessed with an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay in OMM-1 and ARPE-19.
32466624	6	40	theme	acid	919:922	arg1	hydrolysis					924:933	acid hydrolysis	919:933	acid hydrolysis	919:933	Fucoidan composition was analyzed by high performance anion exchange chromatography (HPAEC) after acid hydrolysis.
32466624	0	41	from	Cells	115:119	arg1	Activities					81:90	the Biological Activities	66:90	the Biological Activities of Fucoidans in Ocular Cells	66:119	Effects of a Newly Developed Enzyme-Assisted Extraction Method on the Biological Activities of Fucoidans in Ocular Cells.
32466624	8	42	theme	3-carboxymethoxyphenyl	1120:1141	arg1	assay					1183:1187	an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay	1085:1187	an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay	1085:1187	Cell viability was assessed with an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay in OMM-1 and ARPE-19.
32466624	12	43	theme	OMM-1	1427:1431	arg1	SL_F2					1433:1437	OMM-1 SL_F2	1427:1437	OMM-1 SL_F2	1427:1437	In OMM-1 SL_F2 and several FE fucoidans were protective.
32466624	13	44	dep	FE_SiAT3	1537:1544	arg1	lyase					1586:1590	Sigma-Aldrich alginate lyase	1563:1590	Sigma-Aldrich alginate lyase	1563:1590	LD_SiAT2 (Cellic®CTec2 + Sigma-Aldrich alginate lyase), FE_SiAT3 (Cellic® CTec3 + Sigma-Aldrich alginate lyase), SL_F2 and SL_F3 inhibited VEGF with the latter two as the most effective.
32466624	13	44	dep	FE_SiAT3	1537:1544	arg1	CTec3					1555:1559	Cellic® CTec3	1547:1559	Cellic® CTec3	1547:1559	LD_SiAT2 (Cellic®CTec2 + Sigma-Aldrich alginate lyase), FE_SiAT3 (Cellic® CTec3 + Sigma-Aldrich alginate lyase), SL_F2 and SL_F3 inhibited VEGF with the latter two as the most effective.
32466624	8	45	theme	-2-	1143:1145	arg1	assay					1183:1187	an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay	1085:1187	an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay	1085:1187	Cell viability was assessed with an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay in OMM-1 and ARPE-19.
32466624	10	46	theme	enzyme-linked	1336:1348	arg1	assay					1364:1368	enzyme-linked immunosorbent assay	1336:1368	enzyme-linked immunosorbent assay	1336:1368	Secreted VEGF was analyzed via ELISA (enzyme-linked immunosorbent assay) in ARPE-19 cells.
32466624	10	46	theme	enzyme-linked	1336:1348	arg1	ELISA					1329:1333	ELISA	1329:1333	ELISA (enzyme-linked immunosorbent assay) in ARPE-19 cells	1329:1386	Secreted VEGF was analyzed via ELISA (enzyme-linked immunosorbent assay) in ARPE-19 cells.
32466624	2	47	theme	intensive	284:292	arg1	research					294:301	intensive research in order to find suitable species and extraction methods	284:358	intensive research in order to find suitable species and extraction methods	284:358	The heterogeneity of fucoidans requires intensive research in order to find suitable species and extraction methods.
32466624	8	48	theme	4-sulfophenyl	1147:1159	arg1	assay					1183:1187	an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay	1085:1187	an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay	1085:1187	Cell viability was assessed with an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay in OMM-1 and ARPE-19.
32466624	6	49	theme	exchange	881:888	arg1	chromatography					890:903	high performance anion exchange chromatography	858:903	high performance anion exchange chromatography (HPAEC)	858:911	Fucoidan composition was analyzed by high performance anion exchange chromatography (HPAEC) after acid hydrolysis.
32466624	6	49	theme	exchange	881:888	arg1	HPAEC					906:910	HPAEC	906:910	HPAEC	906:910	Fucoidan composition was analyzed by high performance anion exchange chromatography (HPAEC) after acid hydrolysis.
32466624	12	50	from	protective	1469:1478	arg1	SL_F2					1433:1437	OMM-1 SL_F2	1427:1437	OMM-1 SL_F2	1427:1437	In OMM-1 SL_F2 and several FE fucoidans were protective.
32466624	12	50	from	protective	1469:1478	arg1	fucoidans					1454:1462	several FE fucoidans	1443:1462	several FE fucoidans	1443:1462	In OMM-1 SL_F2 and several FE fucoidans were protective.
32466624	2	51	theme	suitable	320:327	arg1	species					329:335	suitable species	320:335	suitable species	320:335	The heterogeneity of fucoidans requires intensive research in order to find suitable species and extraction methods.
32466624	1	52	theme	potential	180:188	arg1	drugs					190:194	potential drugs	180:194	potential drugs against age-related macular degeneration (AMD)	180:241	Fucoidans from brown seaweeds are promising substances as potential drugs against age-related macular degeneration (AMD).
32466624	8	53	theme	-2H-tetrazolium	1161:1175	arg1	assay					1183:1187	an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay	1085:1187	an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay	1085:1187	Cell viability was assessed with an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay in OMM-1 and ARPE-19.
32466624	0	54	theme	Extraction	45:54	arg1	Method					56:61	a Newly Developed Enzyme-Assisted Extraction Method	11:61	a Newly Developed Enzyme-Assisted Extraction Method	11:61	Effects of a Newly Developed Enzyme-Assisted Extraction Method on the Biological Activities of Fucoidans in Ocular Cells.
32466624	5	55	theme	different	693:701	arg1	enzymes					703:709	different enzymes	693:709	different enzymes	693:709	For this study crude fucoidans were extracted from seaweeds using different enzymes and SL fucoidans were further separated into three fractions (SL_F1-F3) by ion-exchange chromatography (IEX).
32466624	12	56	from	fucoidans	1454:1462	arg1	protective					1469:1478	protective	1469:1478	protective	1469:1478	In OMM-1 SL_F2 and several FE fucoidans were protective.
32466624	0	57	from	Activities	81:90	arg1	Cells					115:119	Ocular Cells	108:119	Ocular Cells	108:119	Effects of a Newly Developed Enzyme-Assisted Extraction Method on the Biological Activities of Fucoidans in Ocular Cells.
32466624	7	58	contain	contained	955:963	arg2	alginate					965:972	alginate	965:972	alginate	965:972	The crude extracts contained alginate, while two of the fractionated SL fucoidans SL_F2 and SL_F3 were highly pure.
32466624	7	58	contain	contained	955:963	arg1	extracts					946:953	The crude extracts	936:953	The crude extracts	936:953	The crude extracts contained alginate, while two of the fractionated SL fucoidans SL_F2 and SL_F3 were highly pure.
32466624	13	59	theme	Sigma-Aldrich	1506:1518	arg1	lyase					1529:1533	Sigma-Aldrich alginate lyase	1506:1533	Sigma-Aldrich alginate lyase	1506:1533	LD_SiAT2 (Cellic®CTec2 + Sigma-Aldrich alginate lyase), FE_SiAT3 (Cellic® CTec3 + Sigma-Aldrich alginate lyase), SL_F2 and SL_F3 inhibited VEGF with the latter two as the most effective.
32466624	14	60	theme	biological	1820:1829	arg1	activities					1831:1840	beneficial AMD relevant biological activities	1796:1840	beneficial AMD relevant biological activities	1796:1840	We could show that enzyme treated fucoidans in general and the fractionated SL fucoidans SL_F2 and SL_F3 are very promising for beneficial AMD relevant biological activities.
32466624	14	61	theme	AMD	1807:1809	arg1	activities					1831:1840	beneficial AMD relevant biological activities	1796:1840	beneficial AMD relevant biological activities	1796:1840	We could show that enzyme treated fucoidans in general and the fractionated SL fucoidans SL_F2 and SL_F3 are very promising for beneficial AMD relevant biological activities.
32466624	12	62	from	SL_F2	1433:1437	arg1	protective					1469:1478	protective	1469:1478	protective	1469:1478	In OMM-1 SL_F2 and several FE fucoidans were protective.
32466624	14	63	dep	fucoidans	1747:1755	arg1	fucoidans					1747:1755	the fractionated SL fucoidans	1727:1755	the fractionated SL fucoidans SL_F2 and SL_F3	1727:1771	We could show that enzyme treated fucoidans in general and the fractionated SL fucoidans SL_F2 and SL_F3 are very promising for beneficial AMD relevant biological activities.
32466624	14	63	dep	fucoidans	1747:1755	arg1	SL_F3					1767:1771	SL_F3	1767:1771	SL_F3	1767:1771	We could show that enzyme treated fucoidans in general and the fractionated SL fucoidans SL_F2 and SL_F3 are very promising for beneficial AMD relevant biological activities.
32466624	14	63	dep	fucoidans	1747:1755	arg1	SL_F2					1757:1761	SL_F2	1757:1761	SL_F2	1757:1761	We could show that enzyme treated fucoidans in general and the fractionated SL fucoidans SL_F2 and SL_F3 are very promising for beneficial AMD relevant biological activities.
32466624	2	64	theme	fucoidans	265:273	arg1	heterogeneity					248:260	The heterogeneity	244:260	The heterogeneity of fucoidans	244:273	The heterogeneity of fucoidans requires intensive research in order to find suitable species and extraction methods.
32466624	7	65	theme	SL	1005:1006	arg1	fucoidans					1008:1016	the fractionated SL fucoidans	988:1016	the fractionated SL fucoidans SL_F2 and SL_F3	988:1032	The crude extracts contained alginate, while two of the fractionated SL fucoidans SL_F2 and SL_F3 were highly pure.
32466624	7	65	theme	SL	1005:1006	arg1	SL_F3					1028:1032	SL_F3	1028:1032	SL_F3	1028:1032	The crude extracts contained alginate, while two of the fractionated SL fucoidans SL_F2 and SL_F3 were highly pure.
32466624	7	65	theme	SL	1005:1006	arg1	SL_F2					1018:1022	SL_F2	1018:1022	SL_F2	1018:1022	The crude extracts contained alginate, while two of the fractionated SL fucoidans SL_F2 and SL_F3 were highly pure.
32466624	0	66	from	Fucoidans	95:103	arg1	Cells					115:119	Ocular Cells	108:119	Ocular Cells	108:119	Effects of a Newly Developed Enzyme-Assisted Extraction Method on the Biological Activities of Fucoidans in Ocular Cells.
32466624	1	67	theme	brown	137:141	arg1	seaweeds					143:150	brown seaweeds	137:150	brown seaweeds	137:150	Fucoidans from brown seaweeds are promising substances as potential drugs against age-related macular degeneration (AMD).
32466624	8	68	theme	MTS	1178:1180	arg1	assay					1183:1187	an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay	1085:1187	an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay	1085:1187	Cell viability was assessed with an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay in OMM-1 and ARPE-19.
32466624	14	69	from	fucoidans	1702:1710	arg1	general					1715:1721	general	1715:1721	general	1715:1721	We could show that enzyme treated fucoidans in general and the fractionated SL fucoidans SL_F2 and SL_F3 are very promising for beneficial AMD relevant biological activities.
32466624	6	70	theme	Fucoidan	821:828	arg1	composition					830:840	Fucoidan composition	821:840	Fucoidan composition	821:840	Fucoidan composition was analyzed by high performance anion exchange chromatography (HPAEC) after acid hydrolysis.
32466624	0	71	theme	Fucoidans	95:103	arg1	Activities					81:90	the Biological Activities	66:90	the Biological Activities of Fucoidans in Ocular Cells	66:119	Effects of a Newly Developed Enzyme-Assisted Extraction Method on the Biological Activities of Fucoidans in Ocular Cells.
32466624	9	72	theme	insult	1269:1274	arg1	h					1257:1257	24 h	1254:1257	24 h of stress insult in OMM-1 and ARPE-19	1254:1295	Protective effects were investigated after 24 h of stress insult in OMM-1 and ARPE-19.
32466624	3	73	theme	fucoidan	375:382	arg1	samples					384:390	Ten different fucoidan samples	361:390	Ten different fucoidan samples	361:390	Ten different fucoidan samples extracted enzymatically from Laminaria digitata (LD), Saccharina latissima (SL) and Fucus distichus subsp.
32466624	14	74	dep	treated	1694:1700	arg1	enzyme					1687:1692	enzyme	1687:1692	enzyme	1687:1692	We could show that enzyme treated fucoidans in general and the fractionated SL fucoidans SL_F2 and SL_F3 are very promising for beneficial AMD relevant biological activities.
32466624	0	75	theme	Ocular	108:113	arg1	Cells					115:119	Ocular Cells	108:119	Ocular Cells	108:119	Effects of a Newly Developed Enzyme-Assisted Extraction Method on the Biological Activities of Fucoidans in Ocular Cells.
32466624	1	76	from	seaweeds	143:150	arg1	Fucoidans					122:130	Fucoidans	122:130	Fucoidans from brown seaweeds	122:150	Fucoidans from brown seaweeds are promising substances as potential drugs against age-related macular degeneration (AMD).
32466624	1	76	from	seaweeds	143:150	arg1	substances					166:175	promising substances	156:175	promising substances as potential drugs against age-related macular degeneration (AMD)	156:241	Fucoidans from brown seaweeds are promising substances as potential drugs against age-related macular degeneration (AMD).
32466624	7	77	dep	fucoidans	1008:1016	arg1	fucoidans					1008:1016	the fractionated SL fucoidans	988:1016	the fractionated SL fucoidans SL_F2 and SL_F3	988:1032	The crude extracts contained alginate, while two of the fractionated SL fucoidans SL_F2 and SL_F3 were highly pure.
32466624	7	77	dep	fucoidans	1008:1016	arg1	SL_F3					1028:1032	SL_F3	1028:1032	SL_F3	1028:1032	The crude extracts contained alginate, while two of the fractionated SL fucoidans SL_F2 and SL_F3 were highly pure.
32466624	7	77	dep	fucoidans	1008:1016	arg1	SL_F2					1018:1022	SL_F2	1018:1022	SL_F2	1018:1022	The crude extracts contained alginate, while two of the fractionated SL fucoidans SL_F2 and SL_F3 were highly pure.
32466624	7	78	theme	crude	940:944	arg1	extracts					946:953	The crude extracts	936:953	The crude extracts	936:953	The crude extracts contained alginate, while two of the fractionated SL fucoidans SL_F2 and SL_F3 were highly pure.
32466624	4	79	theme	oxidative	541:549	arg1	protection					558:567	oxidative stress protection	541:567	oxidative stress protection	541:567	evanescens (FE) were tested for toxicity, oxidative stress protection and VEGF (vascular endothelial growth factor) inhibition.
32466624	0	80	theme	Enzyme-Assisted	29:43	arg1	Method					56:61	a Newly Developed Enzyme-Assisted Extraction Method	11:61	a Newly Developed Enzyme-Assisted Extraction Method	11:61	Effects of a Newly Developed Enzyme-Assisted Extraction Method on the Biological Activities of Fucoidans in Ocular Cells.
32466624	14	81	theme	fractionated	1731:1742	arg1	fucoidans					1747:1755	the fractionated SL fucoidans	1727:1755	the fractionated SL fucoidans SL_F2 and SL_F3	1727:1771	We could show that enzyme treated fucoidans in general and the fractionated SL fucoidans SL_F2 and SL_F3 are very promising for beneficial AMD relevant biological activities.
32466624	14	81	theme	fractionated	1731:1742	arg1	SL_F3					1767:1771	SL_F3	1767:1771	SL_F3	1767:1771	We could show that enzyme treated fucoidans in general and the fractionated SL fucoidans SL_F2 and SL_F3 are very promising for beneficial AMD relevant biological activities.
32466624	14	81	theme	fractionated	1731:1742	arg1	promising					1782:1790	promising	1782:1790	promising	1782:1790	We could show that enzyme treated fucoidans in general and the fractionated SL fucoidans SL_F2 and SL_F3 are very promising for beneficial AMD relevant biological activities.
32466624	14	81	theme	fractionated	1731:1742	arg1	SL_F2					1757:1761	SL_F2	1757:1761	SL_F2	1757:1761	We could show that enzyme treated fucoidans in general and the fractionated SL fucoidans SL_F2 and SL_F3 are very promising for beneficial AMD relevant biological activities.
32466624	6	82	theme	high	858:861	arg1	chromatography					890:903	high performance anion exchange chromatography	858:903	high performance anion exchange chromatography (HPAEC)	858:911	Fucoidan composition was analyzed by high performance anion exchange chromatography (HPAEC) after acid hydrolysis.
32466624	6	82	theme	high	858:861	arg1	HPAEC					906:910	HPAEC	906:910	HPAEC	906:910	Fucoidan composition was analyzed by high performance anion exchange chromatography (HPAEC) after acid hydrolysis.
32466624	13	83	dep	LD_SiAT2	1481:1488	arg1	Cellic®CTec2					1491:1502	Cellic®CTec2	1491:1502	Cellic®CTec2	1491:1502	LD_SiAT2 (Cellic®CTec2 + Sigma-Aldrich alginate lyase), FE_SiAT3 (Cellic® CTec3 + Sigma-Aldrich alginate lyase), SL_F2 and SL_F3 inhibited VEGF with the latter two as the most effective.
32466624	13	83	dep	LD_SiAT2	1481:1488	arg1	lyase					1529:1533	Sigma-Aldrich alginate lyase	1506:1533	Sigma-Aldrich alginate lyase	1506:1533	LD_SiAT2 (Cellic®CTec2 + Sigma-Aldrich alginate lyase), FE_SiAT3 (Cellic® CTec3 + Sigma-Aldrich alginate lyase), SL_F2 and SL_F3 inhibited VEGF with the latter two as the most effective.
32466624	0	84	theme	Method	56:61	arg1	Effects					0:6	Effects	0:6	Effects of a Newly Developed Enzyme-Assisted Extraction Method on the Biological Activities of Fucoidans in Ocular Cells	0:119	Effects of a Newly Developed Enzyme-Assisted Extraction Method on the Biological Activities of Fucoidans in Ocular Cells.
32466624	10	85	theme	Secreted	1298:1305	arg1	VEGF					1307:1310	Secreted VEGF	1298:1310	Secreted VEGF	1298:1310	Secreted VEGF was analyzed via ELISA (enzyme-linked immunosorbent assay) in ARPE-19 cells.
32466624	2	86	theme	extraction	341:350	arg1	methods					352:358	extraction methods	341:358	extraction methods	341:358	The heterogeneity of fucoidans requires intensive research in order to find suitable species and extraction methods.
32466624	4	87	theme	growth	600:605	arg1	factor					607:612	vascular endothelial growth factor	579:612	vascular endothelial growth factor	579:612	evanescens (FE) were tested for toxicity, oxidative stress protection and VEGF (vascular endothelial growth factor) inhibition.
32466624	4	87	theme	growth	600:605	arg1	VEGF					573:576	VEGF	573:576	VEGF (vascular endothelial growth factor) inhibition	573:624	evanescens (FE) were tested for toxicity, oxidative stress protection and VEGF (vascular endothelial growth factor) inhibition.
32466624	3	88	theme	distichus	482:490	arg1	subsp					492:496	Fucus distichus subsp	476:496	Fucus distichus subsp	476:496	Ten different fucoidan samples extracted enzymatically from Laminaria digitata (LD), Saccharina latissima (SL) and Fucus distichus subsp.
32466624	14	89	theme	treated	1694:1700	arg1	fucoidans					1702:1710	enzyme treated fucoidans	1687:1710	enzyme treated fucoidans in general	1687:1721	We could show that enzyme treated fucoidans in general and the fractionated SL fucoidans SL_F2 and SL_F3 are very promising for beneficial AMD relevant biological activities.
32466624	12	90	theme	several	1443:1449	arg1	fucoidans					1454:1462	several FE fucoidans	1443:1462	several FE fucoidans	1443:1462	In OMM-1 SL_F2 and several FE fucoidans were protective.
33359861	3	0	from	effect	396:401	arg1	microbes					522:529	gut microbes	518:529	gut microbes mediated by TPG	518:545	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	3	0	from	effect	396:401	arg1	echinocaulis					455:466	A. echinocaulis	452:466	A. echinocaulis	452:466	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	3	0	from	effect	396:401	arg1	model					481:485	an RA rat model	471:485	an RA rat model	471:485	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	8	1	theme	RA	1091:1092	arg1	model					1098:1102	the RA rat model	1087:1102	the RA rat model	1087:1102	RESULTS The results showed that TPG from A. echinocaulis could inhibit arthritis, reduce serum IL-1β and TNF-α levels, and improve synovial pathology in the RA rat model but failed to produce the same results in a pseudoaseptic RA rat model.
33359861	1	2	used	used	190:193	arg2	RELEVANCE					151:159	ETHNOPHARMACOLOGICAL RELEVANCE	130:159	ETHNOPHARMACOLOGICAL RELEVANCE Aralia echinocaulis	130:179	ETHNOPHARMACOLOGICAL RELEVANCE Aralia echinocaulis has been used in traditional medicines in China and exhibits good effects on rheumatoid arthritis (RA).
33359861	3	3	from	role	495:498	arg1	microbes					522:529	gut microbes	518:529	gut microbes mediated by TPG	518:545	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	3	3	from	role	495:498	arg1	echinocaulis					455:466	A. echinocaulis	452:466	A. echinocaulis	452:466	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	3	3	from	role	495:498	arg1	model					481:485	an RA rat model	471:485	an RA rat model	471:485	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	4	4	theme	rat	620:622	arg1	model					624:628	a collagen-induced arthritis (CIA) rat model	585:628	a collagen-induced arthritis (CIA) rat model	585:628	MATERIALS AND METHODS In this study, a collagen-induced arthritis (CIA) rat model was constructed and used to evaluate the effects of TPG in vivo.
33359861	11	5	theme	KEGG	1456:1459	arg1	pathway					1461:1467	KEGG pathway and COG function analyses	1456:1493	pathway	1461:1467	KEGG pathway and COG function analyses found that TPG-induced alterations in the gut microbiota might be correlated with the circulatory system, excretory system, metabolic diseases, signaling molecules and interactions, coenzyme transport and metabolism, and nucleotide transport and metabolism.
33359861	1	6	theme	traditional	198:208	arg1	medicines					210:218	traditional medicines	198:218	traditional medicines in China	198:227	ETHNOPHARMACOLOGICAL RELEVANCE Aralia echinocaulis has been used in traditional medicines in China and exhibits good effects on rheumatoid arthritis (RA).
33359861	8	7	theme	rat	1165:1167	arg1	model					1169:1173	a pseudoaseptic RA rat model	1146:1173	a pseudoaseptic RA rat model	1146:1173	RESULTS The results showed that TPG from A. echinocaulis could inhibit arthritis, reduce serum IL-1β and TNF-α levels, and improve synovial pathology in the RA rat model but failed to produce the same results in a pseudoaseptic RA rat model.
33359861	13	8	from	mechanisms	1947:1956	arg1	RA					1968:1969	RA	1968:1969	RA	1968:1969	These results are useful to better understanding the mechanisms of TPG in RA.
33359861	11	9	from	alterations	1518:1528	arg1	microbiota					1541:1550	the gut microbiota	1533:1550	the gut microbiota	1533:1550	KEGG pathway and COG function analyses found that TPG-induced alterations in the gut microbiota might be correlated with the circulatory system, excretory system, metabolic diseases, signaling molecules and interactions, coenzyme transport and metabolism, and nucleotide transport and metabolism.
33359861	12	10	from	echinocaulis	1777:1788	arg1	CONCLUSIONS					1753:1763	CONCLUSIONS	1753:1763	CONCLUSIONS TPG from A. echinocaulis	1753:1788	CONCLUSIONS TPG from A. echinocaulis had significant effects on the RA rat model, which are related to the modulation of the gut microbiota.
33359861	4	11	theme	arthritis	604:612	arg1	model					624:628	a collagen-induced arthritis (CIA) rat model	585:628	a collagen-induced arthritis (CIA) rat model	585:628	MATERIALS AND METHODS In this study, a collagen-induced arthritis (CIA) rat model was constructed and used to evaluate the effects of TPG in vivo.
33359861	2	12	theme	Aralia	302:307	arg1	echinocaulis					309:320	THE STUDY Aralia echinocaulis	292:320	THE STUDY Aralia echinocaulis	292:320	AIM OF THE STUDY Aralia echinocaulis is rich in polysaccharides and glycosides.
33359861	8	13	theme	same	1130:1133	arg1	results					1135:1141	the same results	1126:1141	the same results	1126:1141	RESULTS The results showed that TPG from A. echinocaulis could inhibit arthritis, reduce serum IL-1β and TNF-α levels, and improve synovial pathology in the RA rat model but failed to produce the same results in a pseudoaseptic RA rat model.
33359861	10	14	from	genera	1343:1348	arg1	network					1368:1374	a cooccurrence network	1353:1374	a cooccurrence network	1353:1374	The cooccurrence analysis found 19 out of the 50 most abundant genera in a cooccurrence network, of which 16 showed a positive correlation and 3 showed a negative correlation.
33359861	1	15	theme	good	242:245	arg1	effects					247:253	good effects	242:253	good effects on rheumatoid arthritis (RA)	242:282	ETHNOPHARMACOLOGICAL RELEVANCE Aralia echinocaulis has been used in traditional medicines in China and exhibits good effects on rheumatoid arthritis (RA).
33359861	12	16	theme	microbiota	1882:1891	arg1	modulation					1860:1869	the modulation	1856:1869	the modulation of the gut microbiota	1856:1891	CONCLUSIONS TPG from A. echinocaulis had significant effects on the RA rat model, which are related to the modulation of the gut microbiota.
33359861	1	17	theme	ETHNOPHARMACOLOGICAL	130:149	arg1	RELEVANCE					151:159	ETHNOPHARMACOLOGICAL RELEVANCE	130:159	ETHNOPHARMACOLOGICAL RELEVANCE Aralia echinocaulis	130:179	ETHNOPHARMACOLOGICAL RELEVANCE Aralia echinocaulis has been used in traditional medicines in China and exhibits good effects on rheumatoid arthritis (RA).
33359861	11	18	theme	signaling	1639:1647	arg1	metabolism					1700:1709	metabolism	1700:1709	metabolism	1700:1709	KEGG pathway and COG function analyses found that TPG-induced alterations in the gut microbiota might be correlated with the circulatory system, excretory system, metabolic diseases, signaling molecules and interactions, coenzyme transport and metabolism, and nucleotide transport and metabolism.
33359861	11	18	theme	signaling	1639:1647	arg1	transport					1686:1694	coenzyme transport	1677:1694	coenzyme transport	1677:1694	KEGG pathway and COG function analyses found that TPG-induced alterations in the gut microbiota might be correlated with the circulatory system, excretory system, metabolic diseases, signaling molecules and interactions, coenzyme transport and metabolism, and nucleotide transport and metabolism.
33359861	11	18	theme	signaling	1639:1647	arg1	molecules					1649:1657	signaling molecules	1639:1657	signaling molecules	1639:1657	KEGG pathway and COG function analyses found that TPG-induced alterations in the gut microbiota might be correlated with the circulatory system, excretory system, metabolic diseases, signaling molecules and interactions, coenzyme transport and metabolism, and nucleotide transport and metabolism.
33359861	9	19	theme	16S	1176:1178	arg1	sequencing					1185:1194	16S rRNA sequencing	1176:1194	16S rRNA sequencing	1176:1194	16S rRNA sequencing verified that TPG could modulate the gut microbiota community structure of RA rats.
33359861	3	20	theme	gut	518:520	arg1	microbes					522:529	gut microbes	518:529	gut microbes mediated by TPG	518:545	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	1	21	from	medicines	210:218	arg1	China					223:227	China	223:227	China	223:227	ETHNOPHARMACOLOGICAL RELEVANCE Aralia echinocaulis has been used in traditional medicines in China and exhibits good effects on rheumatoid arthritis (RA).
33359861	1	22	theme	Aralia	161:166	arg1	RELEVANCE					151:159	ETHNOPHARMACOLOGICAL RELEVANCE	130:159	ETHNOPHARMACOLOGICAL RELEVANCE Aralia echinocaulis	130:179	ETHNOPHARMACOLOGICAL RELEVANCE Aralia echinocaulis has been used in traditional medicines in China and exhibits good effects on rheumatoid arthritis (RA).
33359861	5	23	theme	rRNA	699:702	arg1	sequencing					704:713	16S rRNA sequencing	695:713	16S rRNA sequencing	695:713	16S rRNA sequencing was used to detect the changes in the gut microbiota.
33359861	7	24	theme	COG	919:921	arg1	databases					923:931	the KEGG and COG databases	906:931	the KEGG and COG databases	906:931	Microbial functions were predicted using PICRUSt with the KEGG and COG databases.
33359861	12	25	theme	RA	1821:1822	arg1	model					1828:1832	the RA rat model	1817:1832	the RA rat model	1817:1832	CONCLUSIONS TPG from A. echinocaulis had significant effects on the RA rat model, which are related to the modulation of the gut microbiota.
33359861	7	26	theme	KEGG	910:913	arg1	databases					923:931	the KEGG and COG databases	906:931	the KEGG and COG databases	906:931	Microbial functions were predicted using PICRUSt with the KEGG and COG databases.
33359861	2	27	from	rich	325:328	arg1	glycosides					353:362	glycosides	353:362	glycosides	353:362	AIM OF THE STUDY Aralia echinocaulis is rich in polysaccharides and glycosides.
33359861	2	27	from	rich	325:328	arg1	polysaccharides					333:347	polysaccharides	333:347	polysaccharides	333:347	AIM OF THE STUDY Aralia echinocaulis is rich in polysaccharides and glycosides.
33359861	10	28	theme	abundant	1334:1341	arg1	genera					1343:1348	the 50 most abundant genera	1322:1348	the 50 most abundant genera in a cooccurrence network, of which 16 showed a positive correlation	1322:1417	The cooccurrence analysis found 19 out of the 50 most abundant genera in a cooccurrence network, of which 16 showed a positive correlation and 3 showed a negative correlation.
33359861	3	29	from	microbes	522:529	arg1	effect					396:401	the effect	392:401	the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model	392:485	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	3	29	from	microbes	522:529	arg1	role					495:498	the role	491:498	the role of alterations in gut microbes mediated by TPG	491:545	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	0	30	theme	microbiota	106:115	arg1	composition					117:127	the gut microbiota composition	98:127	the gut microbiota composition	98:127	Polysaccharides and glycosides from Aralia echinocaulis protect rats from arthritis by modulating the gut microbiota composition.
33359861	5	31	from	changes	738:744	arg1	microbiota					757:766	the gut microbiota	749:766	the gut microbiota	749:766	16S rRNA sequencing was used to detect the changes in the gut microbiota.
33359861	12	32	theme	significant	1794:1804	arg1	effects					1806:1812	significant effects	1794:1812	significant effects	1794:1812	CONCLUSIONS TPG from A. echinocaulis had significant effects on the RA rat model, which are related to the modulation of the gut microbiota.
33359861	4	33	used	used	650:653	arg2	model					624:628	a collagen-induced arthritis (CIA) rat model	585:628	a collagen-induced arthritis (CIA) rat model	585:628	MATERIALS AND METHODS In this study, a collagen-induced arthritis (CIA) rat model was constructed and used to evaluate the effects of TPG in vivo.
33359861	10	34	theme	cooccurrence	1355:1366	arg1	network					1368:1374	a cooccurrence network	1353:1374	a cooccurrence network	1353:1374	The cooccurrence analysis found 19 out of the 50 most abundant genera in a cooccurrence network, of which 16 showed a positive correlation and 3 showed a negative correlation.
33359861	3	35	theme	glycoside	431:439	arg1	effect					396:401	the effect	392:401	the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model	392:485	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	3	35	theme	glycoside	431:439	arg1	role					495:498	the role	491:498	the role of alterations in gut microbes mediated by TPG	491:545	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	9	36	theme	microbiota	1237:1246	arg1	structure					1258:1266	the gut microbiota community structure	1229:1266	the gut microbiota community structure of RA rats	1229:1277	16S rRNA sequencing verified that TPG could modulate the gut microbiota community structure of RA rats.
33359861	5	37	theme	gut	753:755	arg1	microbiota					757:766	the gut microbiota	749:766	the gut microbiota	749:766	16S rRNA sequencing was used to detect the changes in the gut microbiota.
33359861	3	38	theme	polysaccharide	412:425	arg1	effect					396:401	the effect	392:401	the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model	392:485	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	3	38	theme	polysaccharide	412:425	arg1	role					495:498	the role	491:498	the role of alterations in gut microbes mediated by TPG	491:545	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	8	39	theme	pseudoaseptic	1148:1160	arg1	model					1169:1173	a pseudoaseptic RA rat model	1146:1173	a pseudoaseptic RA rat model	1146:1173	RESULTS The results showed that TPG from A. echinocaulis could inhibit arthritis, reduce serum IL-1β and TNF-α levels, and improve synovial pathology in the RA rat model but failed to produce the same results in a pseudoaseptic RA rat model.
33359861	9	40	theme	RA	1271:1272	arg1	rats					1274:1277	RA rats	1271:1277	RA rats	1271:1277	16S rRNA sequencing verified that TPG could modulate the gut microbiota community structure of RA rats.
33359861	0	41	theme	Aralia	36:41	arg1	echinocaulis					43:54	Aralia echinocaulis	36:54	Aralia echinocaulis	36:54	Polysaccharides and glycosides from Aralia echinocaulis protect rats from arthritis by modulating the gut microbiota composition.
33359861	11	42	theme	circulatory	1581:1591	arg1	system					1593:1598	the circulatory system	1577:1598	the circulatory system	1577:1598	KEGG pathway and COG function analyses found that TPG-induced alterations in the gut microbiota might be correlated with the circulatory system, excretory system, metabolic diseases, signaling molecules and interactions, coenzyme transport and metabolism, and nucleotide transport and metabolism.
33359861	3	43	theme	rat	477:479	arg1	model					481:485	an RA rat model	471:485	an RA rat model	471:485	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	5	44	used	used	719:722	arg2	sequencing					704:713	16S rRNA sequencing	695:713	16S rRNA sequencing	695:713	16S rRNA sequencing was used to detect the changes in the gut microbiota.
33359861	8	45	theme	TNF-α	1039:1043	arg1	levels					1045:1050	serum IL-1β and TNF-α levels	1023:1050	levels	1045:1050	RESULTS The results showed that TPG from A. echinocaulis could inhibit arthritis, reduce serum IL-1β and TNF-α levels, and improve synovial pathology in the RA rat model but failed to produce the same results in a pseudoaseptic RA rat model.
33359861	6	46	theme	cooccurrence	771:782	arg1	analysis					784:791	A cooccurrence analysis	769:791	A cooccurrence analysis	769:791	A cooccurrence analysis was conducted by calculating Spearman's rank correlations.
33359861	10	47	theme	positive	1398:1405	arg1	correlation					1407:1417	a positive correlation	1396:1417	a positive correlation	1396:1417	The cooccurrence analysis found 19 out of the 50 most abundant genera in a cooccurrence network, of which 16 showed a positive correlation and 3 showed a negative correlation.
33359861	11	48	theme	metabolic	1619:1627	arg1	diseases					1629:1636	metabolic diseases	1619:1636	metabolic diseases	1619:1636	KEGG pathway and COG function analyses found that TPG-induced alterations in the gut microbiota might be correlated with the circulatory system, excretory system, metabolic diseases, signaling molecules and interactions, coenzyme transport and metabolism, and nucleotide transport and metabolism.
33359861	11	49	theme	COG	1473:1475	arg1	analyses					1486:1493	KEGG pathway and COG function analyses	1456:1493	analyses	1486:1493	KEGG pathway and COG function analyses found that TPG-induced alterations in the gut microbiota might be correlated with the circulatory system, excretory system, metabolic diseases, signaling molecules and interactions, coenzyme transport and metabolism, and nucleotide transport and metabolism.
33359861	10	50	theme	negative	1434:1441	arg1	correlation					1443:1453	a negative correlation	1432:1453	a negative correlation	1432:1453	The cooccurrence analysis found 19 out of the 50 most abundant genera in a cooccurrence network, of which 16 showed a positive correlation and 3 showed a negative correlation.
33359861	1	51	theme	rheumatoid	258:267	arg1	RA					280:281	RA	280:281	RA	280:281	ETHNOPHARMACOLOGICAL RELEVANCE Aralia echinocaulis has been used in traditional medicines in China and exhibits good effects on rheumatoid arthritis (RA).
33359861	1	51	theme	rheumatoid	258:267	arg1	arthritis					269:277	rheumatoid arthritis	258:277	rheumatoid arthritis (RA)	258:282	ETHNOPHARMACOLOGICAL RELEVANCE Aralia echinocaulis has been used in traditional medicines in China and exhibits good effects on rheumatoid arthritis (RA).
33359861	2	52	from	polysaccharides	333:347	arg1	rich					325:328	rich	325:328	rich	325:328	AIM OF THE STUDY Aralia echinocaulis is rich in polysaccharides and glycosides.
33359861	2	53	theme	STUDY	296:300	arg1	echinocaulis					309:320	THE STUDY Aralia echinocaulis	292:320	THE STUDY Aralia echinocaulis	292:320	AIM OF THE STUDY Aralia echinocaulis is rich in polysaccharides and glycosides.
33359861	10	54	dep	found	1306:1310	arg1	showed					1425:1430	showed	1425:1430	found 19 out of the 50 most abundant genera in a cooccurrence network, of which 16 showed a positive correlation and 3 showed a negative correlation	1306:1453	The cooccurrence analysis found 19 out of the 50 most abundant genera in a cooccurrence network, of which 16 showed a positive correlation and 3 showed a negative correlation.
33359861	12	55	theme	gut	1878:1880	arg1	microbiota					1882:1891	the gut microbiota	1874:1891	the gut microbiota	1874:1891	CONCLUSIONS TPG from A. echinocaulis had significant effects on the RA rat model, which are related to the modulation of the gut microbiota.
33359861	6	56	theme	rank	833:836	arg1	correlations					838:849	Spearman's rank correlations	822:849	Spearman's rank correlations	822:849	A cooccurrence analysis was conducted by calculating Spearman's rank correlations.
33359861	2	57	from	glycosides	353:362	arg1	rich					325:328	rich	325:328	rich	325:328	AIM OF THE STUDY Aralia echinocaulis is rich in polysaccharides and glycosides.
33359861	10	58	theme	cooccurrence	1284:1295	arg1	analysis					1297:1304	The cooccurrence analysis	1280:1304	The cooccurrence analysis	1280:1304	The cooccurrence analysis found 19 out of the 50 most abundant genera in a cooccurrence network, of which 16 showed a positive correlation and 3 showed a negative correlation.
33359861	3	59	theme	alterations	503:513	arg1	effect					396:401	the effect	392:401	the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model	392:485	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	3	59	theme	alterations	503:513	arg1	role					495:498	the role	491:498	the role of alterations in gut microbes mediated by TPG	491:545	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	8	60	theme	rat	1094:1096	arg1	model					1098:1102	the RA rat model	1087:1102	the RA rat model	1087:1102	RESULTS The results showed that TPG from A. echinocaulis could inhibit arthritis, reduce serum IL-1β and TNF-α levels, and improve synovial pathology in the RA rat model but failed to produce the same results in a pseudoaseptic RA rat model.
33359861	8	61	from	echinocaulis	978:989	arg1	TPG					966:968	TPG	966:968	TPG from A. echinocaulis	966:989	RESULTS The results showed that TPG from A. echinocaulis could inhibit arthritis, reduce serum IL-1β and TNF-α levels, and improve synovial pathology in the RA rat model but failed to produce the same results in a pseudoaseptic RA rat model.
33359861	8	62	theme	RA	1162:1163	arg1	model					1169:1173	a pseudoaseptic RA rat model	1146:1173	a pseudoaseptic RA rat model	1146:1173	RESULTS The results showed that TPG from A. echinocaulis could inhibit arthritis, reduce serum IL-1β and TNF-α levels, and improve synovial pathology in the RA rat model but failed to produce the same results in a pseudoaseptic RA rat model.
33359861	2	63	theme	echinocaulis	309:320	arg1	AIM					285:287	AIM	285:287	AIM OF THE STUDY Aralia echinocaulis	285:320	AIM OF THE STUDY Aralia echinocaulis is rich in polysaccharides and glycosides.
33359861	4	64	theme	collagen-induced	587:602	arg1	CIA					615:617	CIA	615:617	CIA	615:617	MATERIALS AND METHODS In this study, a collagen-induced arthritis (CIA) rat model was constructed and used to evaluate the effects of TPG in vivo.
33359861	4	64	theme	collagen-induced	587:602	arg1	arthritis					604:612	collagen-induced arthritis	587:612	a collagen-induced arthritis (CIA) rat model	585:628	MATERIALS AND METHODS In this study, a collagen-induced arthritis (CIA) rat model was constructed and used to evaluate the effects of TPG in vivo.
33359861	0	65	from	arthritis	74:82	arg1	rats					64:67	rats	64:67	rats from arthritis	64:82	Polysaccharides and glycosides from Aralia echinocaulis protect rats from arthritis by modulating the gut microbiota composition.
33359861	4	66	dep	MATERIALS	548:556	arg1	used					650:653	used	650:653	used	650:653	MATERIALS AND METHODS In this study, a collagen-induced arthritis (CIA) rat model was constructed and used to evaluate the effects of TPG in vivo.
33359861	4	66	dep	MATERIALS	548:556	arg1	constructed					634:644	constructed	634:644	constructed	634:644	MATERIALS AND METHODS In this study, a collagen-induced arthritis (CIA) rat model was constructed and used to evaluate the effects of TPG in vivo.
33359861	11	67	theme	nucleotide	1716:1725	arg1	transport					1727:1735	nucleotide transport	1716:1735	nucleotide transport	1716:1735	KEGG pathway and COG function analyses found that TPG-induced alterations in the gut microbiota might be correlated with the circulatory system, excretory system, metabolic diseases, signaling molecules and interactions, coenzyme transport and metabolism, and nucleotide transport and metabolism.
33359861	1	68	from	effects	247:253	arg1	RA					280:281	RA	280:281	RA	280:281	ETHNOPHARMACOLOGICAL RELEVANCE Aralia echinocaulis has been used in traditional medicines in China and exhibits good effects on rheumatoid arthritis (RA).
33359861	1	68	from	effects	247:253	arg1	arthritis					269:277	rheumatoid arthritis	258:277	rheumatoid arthritis (RA)	258:282	ETHNOPHARMACOLOGICAL RELEVANCE Aralia echinocaulis has been used in traditional medicines in China and exhibits good effects on rheumatoid arthritis (RA).
33359861	5	69	theme	16S	695:697	arg1	sequencing					704:713	16S rRNA sequencing	695:713	16S rRNA sequencing	695:713	16S rRNA sequencing was used to detect the changes in the gut microbiota.
33359861	9	70	theme	rRNA	1180:1183	arg1	sequencing					1185:1194	16S rRNA sequencing	1176:1194	16S rRNA sequencing	1176:1194	16S rRNA sequencing verified that TPG could modulate the gut microbiota community structure of RA rats.
33359861	3	71	from	alterations	503:513	arg1	microbes					522:529	gut microbes	518:529	gut microbes mediated by TPG	518:545	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	13	72	theme	TPG	1961:1963	arg1	mechanisms					1947:1956	the mechanisms	1943:1956	the mechanisms of TPG in RA	1943:1969	These results are useful to better understanding the mechanisms of TPG in RA.
33359861	12	73	theme	rat	1824:1826	arg1	model					1828:1832	the RA rat model	1817:1832	the RA rat model	1817:1832	CONCLUSIONS TPG from A. echinocaulis had significant effects on the RA rat model, which are related to the modulation of the gut microbiota.
33359861	11	74	theme	coenzyme	1677:1684	arg1	transport					1686:1694	coenzyme transport	1677:1694	coenzyme transport	1677:1694	KEGG pathway and COG function analyses found that TPG-induced alterations in the gut microbiota might be correlated with the circulatory system, excretory system, metabolic diseases, signaling molecules and interactions, coenzyme transport and metabolism, and nucleotide transport and metabolism.
33359861	11	74	theme	coenzyme	1677:1684	arg1	molecules					1649:1657	signaling molecules	1639:1657	signaling molecules	1639:1657	KEGG pathway and COG function analyses found that TPG-induced alterations in the gut microbiota might be correlated with the circulatory system, excretory system, metabolic diseases, signaling molecules and interactions, coenzyme transport and metabolism, and nucleotide transport and metabolism.
33359861	0	75	theme	gut	102:104	arg1	composition					117:127	the gut microbiota composition	98:127	the gut microbiota composition	98:127	Polysaccharides and glycosides from Aralia echinocaulis protect rats from arthritis by modulating the gut microbiota composition.
33359861	12	76	from	effects	1806:1812	arg1	model					1828:1832	the RA rat model	1817:1832	the RA rat model	1817:1832	CONCLUSIONS TPG from A. echinocaulis had significant effects on the RA rat model, which are related to the modulation of the gut microbiota.
33359861	1	77	dep	Aralia	161:166	arg1	echinocaulis					168:179	echinocaulis	168:179	echinocaulis	168:179	ETHNOPHARMACOLOGICAL RELEVANCE Aralia echinocaulis has been used in traditional medicines in China and exhibits good effects on rheumatoid arthritis (RA).
33359861	0	78	from	echinocaulis	43:54	arg1	glycosides					20:29	glycosides	20:29	glycosides	20:29	Polysaccharides and glycosides from Aralia echinocaulis protect rats from arthritis by modulating the gut microbiota composition.
33359861	0	78	from	echinocaulis	43:54	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides	0:14	Polysaccharides and glycosides from Aralia echinocaulis protect rats from arthritis by modulating the gut microbiota composition.
33359861	9	79	theme	gut	1233:1235	arg1	structure					1258:1266	the gut microbiota community structure	1229:1266	the gut microbiota community structure of RA rats	1229:1277	16S rRNA sequencing verified that TPG could modulate the gut microbiota community structure of RA rats.
33359861	4	80	theme	TPG	682:684	arg1	effects					671:677	the effects	667:677	the effects of TPG	667:684	MATERIALS AND METHODS In this study, a collagen-induced arthritis (CIA) rat model was constructed and used to evaluate the effects of TPG in vivo.
33359861	8	81	dep	RESULTS	934:940	arg1	showed					954:959	showed	954:959	showed that TPG from A. echinocaulis could inhibit arthritis, reduce serum IL-1β and TNF-α levels, and improve synovial pathology in the RA rat model but failed to produce the same results in a pseudoaseptic RA rat model	954:1173	RESULTS The results showed that TPG from A. echinocaulis could inhibit arthritis, reduce serum IL-1β and TNF-α levels, and improve synovial pathology in the RA rat model but failed to produce the same results in a pseudoaseptic RA rat model.
33359861	9	82	theme	community	1248:1256	arg1	structure					1258:1266	the gut microbiota community structure	1229:1266	the gut microbiota community structure of RA rats	1229:1277	16S rRNA sequencing verified that TPG could modulate the gut microbiota community structure of RA rats.
33359861	8	83	theme	serum	1023:1027	arg1	IL-1β					1029:1033	serum IL-1β and TNF-α levels	1023:1050	IL-1β	1029:1033	RESULTS The results showed that TPG from A. echinocaulis could inhibit arthritis, reduce serum IL-1β and TNF-α levels, and improve synovial pathology in the RA rat model but failed to produce the same results in a pseudoaseptic RA rat model.
33359861	8	84	from	pathology	1074:1082	arg1	model					1098:1102	the RA rat model	1087:1102	the RA rat model	1087:1102	RESULTS The results showed that TPG from A. echinocaulis could inhibit arthritis, reduce serum IL-1β and TNF-α levels, and improve synovial pathology in the RA rat model but failed to produce the same results in a pseudoaseptic RA rat model.
33359861	11	85	theme	function	1477:1484	arg1	analyses					1486:1493	KEGG pathway and COG function analyses	1456:1493	analyses	1486:1493	KEGG pathway and COG function analyses found that TPG-induced alterations in the gut microbiota might be correlated with the circulatory system, excretory system, metabolic diseases, signaling molecules and interactions, coenzyme transport and metabolism, and nucleotide transport and metabolism.
33359861	9	86	theme	rats	1274:1277	arg1	structure					1258:1266	the gut microbiota community structure	1229:1266	the gut microbiota community structure of RA rats	1229:1277	16S rRNA sequencing verified that TPG could modulate the gut microbiota community structure of RA rats.
33359861	3	87	theme	total	406:410	arg1	TPG					442:444	TPG	442:444	TPG	442:444	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	3	87	theme	total	406:410	arg1	polysaccharide					412:425	total polysaccharide	406:425	total polysaccharide	406:425	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	3	88	from	echinocaulis	455:466	arg1	TPG					442:444	TPG	442:444	TPG	442:444	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	3	88	from	echinocaulis	455:466	arg1	polysaccharide					412:425	total polysaccharide	406:425	total polysaccharide	406:425	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	3	88	from	echinocaulis	455:466	arg1	glycoside					431:439	glycoside	431:439	glycoside	431:439	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	3	88	from	echinocaulis	455:466	arg1	effect					396:401	the effect	392:401	the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model	392:485	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	3	88	from	echinocaulis	455:466	arg1	role					495:498	the role	491:498	the role of alterations in gut microbes mediated by TPG	491:545	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	7	89	theme	Microbial	852:860	arg1	functions					862:870	Microbial functions	852:870	Microbial functions	852:870	Microbial functions were predicted using PICRUSt with the KEGG and COG databases.
33359861	11	90	theme	excretory	1601:1609	arg1	system					1611:1616	excretory system	1601:1616	excretory system	1601:1616	KEGG pathway and COG function analyses found that TPG-induced alterations in the gut microbiota might be correlated with the circulatory system, excretory system, metabolic diseases, signaling molecules and interactions, coenzyme transport and metabolism, and nucleotide transport and metabolism.
33359861	11	91	theme	TPG-induced	1506:1516	arg1	alterations					1518:1528	TPG-induced alterations	1506:1528	TPG-induced alterations in the gut microbiota	1506:1550	KEGG pathway and COG function analyses found that TPG-induced alterations in the gut microbiota might be correlated with the circulatory system, excretory system, metabolic diseases, signaling molecules and interactions, coenzyme transport and metabolism, and nucleotide transport and metabolism.
33359861	3	92	theme	RA	474:475	arg1	model					481:485	an RA rat model	471:485	an RA rat model	471:485	This study aims to explore the effect of total polysaccharide and glycoside (TPG) from A. echinocaulis on an RA rat model and the role of alterations in gut microbes mediated by TPG.
33359861	8	93	theme	synovial	1065:1072	arg1	pathology					1074:1082	synovial pathology	1065:1082	synovial pathology in the RA rat model	1065:1102	RESULTS The results showed that TPG from A. echinocaulis could inhibit arthritis, reduce serum IL-1β and TNF-α levels, and improve synovial pathology in the RA rat model but failed to produce the same results in a pseudoaseptic RA rat model.
33359861	11	94	theme	gut	1537:1539	arg1	microbiota					1541:1550	the gut microbiota	1533:1550	the gut microbiota	1533:1550	KEGG pathway and COG function analyses found that TPG-induced alterations in the gut microbiota might be correlated with the circulatory system, excretory system, metabolic diseases, signaling molecules and interactions, coenzyme transport and metabolism, and nucleotide transport and metabolism.
33359861	12	95	contain	had	1790:1792	arg1	CONCLUSIONS					1753:1763	CONCLUSIONS	1753:1763	CONCLUSIONS TPG from A. echinocaulis	1753:1788	CONCLUSIONS TPG from A. echinocaulis had significant effects on the RA rat model, which are related to the modulation of the gut microbiota.
33359861	12	95	contain	had	1790:1792	arg2	effects					1806:1812	significant effects	1794:1812	significant effects	1794:1812	CONCLUSIONS TPG from A. echinocaulis had significant effects on the RA rat model, which are related to the modulation of the gut microbiota.
32828888	0	0	theme	overripe	65:72	arg1	papayas					74:80	ripe and overripe papayas	56:80	ripe and overripe papayas	56:80	The acid and neutral fractions of pectins isolated from ripe and overripe papayas differentially affect galectin-3 inhibition and colon cancer cell growth.
32828888	6	1	theme	viability	1292:1300	arg1	assays					1302:1307	viability assays	1292:1307	viability assays	1292:1307	The structures of the fractions were chemically characterized, and the uronic fraction extracted from the fourth day after harvesting presented the best biological effects across different concentrations in both galectin-3 inhibition and viability assays.
32828888	6	2	from	concentrations	1243:1256	arg1	inhibition					1277:1286	galectin-3 inhibition	1266:1286	galectin-3 inhibition	1266:1286	The structures of the fractions were chemically characterized, and the uronic fraction extracted from the fourth day after harvesting presented the best biological effects across different concentrations in both galectin-3 inhibition and viability assays.
32828888	6	2	from	concentrations	1243:1256	arg1	assays					1302:1307	viability assays	1292:1307	viability assays	1292:1307	The structures of the fractions were chemically characterized, and the uronic fraction extracted from the fourth day after harvesting presented the best biological effects across different concentrations in both galectin-3 inhibition and viability assays.
32828888	7	3	theme	cancer	1483:1488	arg1	growth					1495:1500	cancer cell growth	1483:1500	cancer cell growth	1483:1500	The results obtained may help to establish a relationship between the chemical structures of papaya pectins and the positive in vitro biological effects, such as inhibiting cancer cell growth.
32828888	6	4	theme	biological	1207:1216	arg1	effects					1218:1224	the best biological effects	1198:1224	the best biological effects	1198:1224	The structures of the fractions were chemically characterized, and the uronic fraction extracted from the fourth day after harvesting presented the best biological effects across different concentrations in both galectin-3 inhibition and viability assays.
32828888	7	5	theme	pectins	1410:1416	arg1	effects					1455:1461	the positive in vitro biological effects	1422:1461	the positive in vitro biological effects	1422:1461	The results obtained may help to establish a relationship between the chemical structures of papaya pectins and the positive in vitro biological effects, such as inhibiting cancer cell growth.
32828888	7	5	theme	pectins	1410:1416	arg1	structures					1389:1398	the chemical structures	1376:1398	the chemical structures of papaya pectins	1376:1416	The results obtained may help to establish a relationship between the chemical structures of papaya pectins and the positive in vitro biological effects, such as inhibiting cancer cell growth.
32828888	5	6	theme	neutral	920:926	arg1	fractions					928:936	uronic and neutral fractions	909:936	uronic and neutral fractions	909:936	The aims of this work are to fractionate the pectin extracted from the pulp of papayas at two stages of ripening (fourth and ninth day after harvesting) into uronic and neutral fractions and to test them for the inhibition of human recombinant galectin-3 and the inhibition of colon cancer cell growth.
32828888	6	7	theme	galectin-3	1266:1275	arg1	inhibition					1277:1286	galectin-3 inhibition	1266:1286	galectin-3 inhibition	1266:1286	The structures of the fractions were chemically characterized, and the uronic fraction extracted from the fourth day after harvesting presented the best biological effects across different concentrations in both galectin-3 inhibition and viability assays.
32828888	6	8	theme	fourth	1160:1165	arg1	day					1167:1169	the fourth day	1156:1169	the fourth day after harvesting	1156:1186	The structures of the fractions were chemically characterized, and the uronic fraction extracted from the fourth day after harvesting presented the best biological effects across different concentrations in both galectin-3 inhibition and viability assays.
32828888	5	9	theme	ripening	855:862	arg1	stages					845:850	two stages	841:850	two stages of ripening (fourth and ninth day after harvesting)	841:902	The aims of this work are to fractionate the pectin extracted from the pulp of papayas at two stages of ripening (fourth and ninth day after harvesting) into uronic and neutral fractions and to test them for the inhibition of human recombinant galectin-3 and the inhibition of colon cancer cell growth.
32828888	0	10	theme	galectin-3	104:113	arg1	inhibition					115:124	galectin-3 inhibition	104:124	galectin-3 inhibition	104:124	The acid and neutral fractions of pectins isolated from ripe and overripe papayas differentially affect galectin-3 inhibition and colon cancer cell growth.
32828888	7	11	theme	positive	1426:1433	arg1	effects					1455:1461	the positive in vitro biological effects	1422:1461	the positive in vitro biological effects	1422:1461	The results obtained may help to establish a relationship between the chemical structures of papaya pectins and the positive in vitro biological effects, such as inhibiting cancer cell growth.
32828888	1	12	theme	water-soluble	160:172	arg1	fractions					174:182	The water-soluble fractions	156:182	The water-soluble fractions of pectin extracted from the pulp of ripe papayas	156:232	The water-soluble fractions of pectin extracted from the pulp of ripe papayas have already been found to exert positive effects on cancer cell cultures.
32828888	3	13	theme	structural	529:538	arg1	conformation					540:551	structural conformation	529:551	structural conformation	529:551	Characteristics such as molecular size, monosaccharide composition and structural conformation are known as polysaccharide factors that can cause alterations in cellular response.
32828888	0	14	theme	acid	4:7	arg1	fractions					21:29	The acid and neutral fractions	0:29	fractions	21:29	The acid and neutral fractions of pectins isolated from ripe and overripe papayas differentially affect galectin-3 inhibition and colon cancer cell growth.
32828888	5	15	theme	work	768:771	arg1	aims					755:758	The aims	751:758	The aims of this work	751:771	The aims of this work are to fractionate the pectin extracted from the pulp of papayas at two stages of ripening (fourth and ninth day after harvesting) into uronic and neutral fractions and to test them for the inhibition of human recombinant galectin-3 and the inhibition of colon cancer cell growth.
32828888	3	16	theme	polysaccharide	566:579	arg1	conformation					540:551	structural conformation	529:551	structural conformation	529:551	Characteristics such as molecular size, monosaccharide composition and structural conformation are known as polysaccharide factors that can cause alterations in cellular response.
32828888	3	16	theme	polysaccharide	566:579	arg1	Characteristics					458:472	Characteristics	458:472	Characteristics such as molecular size, monosaccharide composition and structural conformation	458:551	Characteristics such as molecular size, monosaccharide composition and structural conformation are known as polysaccharide factors that can cause alterations in cellular response.
32828888	3	16	theme	polysaccharide	566:579	arg1	composition					513:523	monosaccharide composition	498:523	monosaccharide composition	498:523	Characteristics such as molecular size, monosaccharide composition and structural conformation are known as polysaccharide factors that can cause alterations in cellular response.
32828888	3	16	theme	polysaccharide	566:579	arg1	size					492:495	molecular size	482:495	molecular size	482:495	Characteristics such as molecular size, monosaccharide composition and structural conformation are known as polysaccharide factors that can cause alterations in cellular response.
32828888	3	16	theme	polysaccharide	566:579	arg1	factors					581:587	polysaccharide factors	566:587	polysaccharide factors that can cause alterations in cellular response	566:635	Characteristics such as molecular size, monosaccharide composition and structural conformation are known as polysaccharide factors that can cause alterations in cellular response.
32828888	5	17	theme	fourth	865:870	arg1	day					882:884	fourth and ninth day	865:884	fourth and ninth day after harvesting	865:901	The aims of this work are to fractionate the pectin extracted from the pulp of papayas at two stages of ripening (fourth and ninth day after harvesting) into uronic and neutral fractions and to test them for the inhibition of human recombinant galectin-3 and the inhibition of colon cancer cell growth.
32828888	6	18	theme	different	1233:1241	arg1	concentrations					1243:1256	different concentrations	1233:1256	different concentrations in both galectin-3 inhibition and viability assays	1233:1307	The structures of the fractions were chemically characterized, and the uronic fraction extracted from the fourth day after harvesting presented the best biological effects across different concentrations in both galectin-3 inhibition and viability assays.
32828888	2	19	theme	beneficial	352:361	arg1	effects					363:369	these beneficial effects	346:369	these beneficial effects	346:369	However, the mechanisms that lead to these beneficial effects and the pectin characteristics that exert these effects are still not well understood.
32828888	0	20	theme	neutral	13:19	arg1	fractions					21:29	The acid and neutral fractions	0:29	fractions	21:29	The acid and neutral fractions of pectins isolated from ripe and overripe papayas differentially affect galectin-3 inhibition and colon cancer cell growth.
32828888	1	21	theme	positive	267:274	arg1	effects					276:282	positive effects	267:282	positive effects	267:282	The water-soluble fractions of pectin extracted from the pulp of ripe papayas have already been found to exert positive effects on cancer cell cultures.
32828888	0	22	attach	isolated	42:49	arg2	pectins					34:40	pectins	34:40	pectins isolated from ripe and overripe papayas	34:80	The acid and neutral fractions of pectins isolated from ripe and overripe papayas differentially affect galectin-3 inhibition and colon cancer cell growth.
32828888	0	22	attach	isolated	42:49	arg1	papayas					74:80	ripe and overripe papayas	56:80	ripe and overripe papayas	56:80	The acid and neutral fractions of pectins isolated from ripe and overripe papayas differentially affect galectin-3 inhibition and colon cancer cell growth.
32828888	6	23	theme	uronic	1125:1130	arg1	fraction					1132:1139	the uronic fraction	1121:1139	the uronic fraction extracted from the fourth day after harvesting	1121:1186	The structures of the fractions were chemically characterized, and the uronic fraction extracted from the fourth day after harvesting presented the best biological effects across different concentrations in both galectin-3 inhibition and viability assays.
32828888	4	24	theme	fruit	645:649	arg1	ripening					651:658	fruit ripening	645:658	fruit ripening	645:658	During fruit ripening, a major polysaccharide solubilization, depolymerization, and chemical modification occur.
32828888	5	25	theme	papayas	830:836	arg1	pulp					822:825	the pulp	818:825	the pulp of papayas	818:836	The aims of this work are to fractionate the pectin extracted from the pulp of papayas at two stages of ripening (fourth and ninth day after harvesting) into uronic and neutral fractions and to test them for the inhibition of human recombinant galectin-3 and the inhibition of colon cancer cell growth.
32828888	5	26	theme	growth	1046:1051	arg1	inhibition					963:972	the inhibition	959:972	the inhibition of human recombinant galectin-3	959:1004	The aims of this work are to fractionate the pectin extracted from the pulp of papayas at two stages of ripening (fourth and ninth day after harvesting) into uronic and neutral fractions and to test them for the inhibition of human recombinant galectin-3 and the inhibition of colon cancer cell growth.
32828888	5	26	theme	growth	1046:1051	arg1	inhibition					1014:1023	the inhibition	1010:1023	the inhibition of colon cancer cell growth	1010:1051	The aims of this work are to fractionate the pectin extracted from the pulp of papayas at two stages of ripening (fourth and ninth day after harvesting) into uronic and neutral fractions and to test them for the inhibition of human recombinant galectin-3 and the inhibition of colon cancer cell growth.
32828888	3	27	from	alterations	604:614	arg1	response					628:635	cellular response	619:635	cellular response	619:635	Characteristics such as molecular size, monosaccharide composition and structural conformation are known as polysaccharide factors that can cause alterations in cellular response.
32828888	5	28	theme	ninth	876:880	arg1	day					882:884	fourth and ninth day	865:884	fourth and ninth day after harvesting	865:901	The aims of this work are to fractionate the pectin extracted from the pulp of papayas at two stages of ripening (fourth and ninth day after harvesting) into uronic and neutral fractions and to test them for the inhibition of human recombinant galectin-3 and the inhibition of colon cancer cell growth.
32828888	4	29	theme	chemical	722:729	arg1	modification					731:742	chemical modification	722:742	chemical modification	722:742	During fruit ripening, a major polysaccharide solubilization, depolymerization, and chemical modification occur.
32828888	0	30	theme	cancer	136:141	arg1	growth					148:153	colon cancer cell growth	130:153	colon cancer cell growth	130:153	The acid and neutral fractions of pectins isolated from ripe and overripe papayas differentially affect galectin-3 inhibition and colon cancer cell growth.
32828888	5	31	theme	galectin-3	995:1004	arg1	inhibition					963:972	the inhibition	959:972	the inhibition of human recombinant galectin-3	959:1004	The aims of this work are to fractionate the pectin extracted from the pulp of papayas at two stages of ripening (fourth and ninth day after harvesting) into uronic and neutral fractions and to test them for the inhibition of human recombinant galectin-3 and the inhibition of colon cancer cell growth.
32828888	5	31	theme	galectin-3	995:1004	arg1	inhibition					1014:1023	the inhibition	1010:1023	the inhibition of colon cancer cell growth	1010:1051	The aims of this work are to fractionate the pectin extracted from the pulp of papayas at two stages of ripening (fourth and ninth day after harvesting) into uronic and neutral fractions and to test them for the inhibition of human recombinant galectin-3 and the inhibition of colon cancer cell growth.
32828888	1	32	theme	pectin	187:192	arg1	fractions					174:182	The water-soluble fractions	156:182	The water-soluble fractions of pectin extracted from the pulp of ripe papayas	156:232	The water-soluble fractions of pectin extracted from the pulp of ripe papayas have already been found to exert positive effects on cancer cell cultures.
32828888	7	33	theme	biological	1444:1453	arg1	effects					1455:1461	the positive in vitro biological effects	1422:1461	the positive in vitro biological effects	1422:1461	The results obtained may help to establish a relationship between the chemical structures of papaya pectins and the positive in vitro biological effects, such as inhibiting cancer cell growth.
32828888	4	34	theme	major	663:667	arg1	solubilization					684:697	a major polysaccharide solubilization	661:697	a major polysaccharide solubilization	661:697	During fruit ripening, a major polysaccharide solubilization, depolymerization, and chemical modification occur.
32828888	1	35	theme	cancer	287:292	arg1	cultures					299:306	cancer cell cultures	287:306	cancer cell cultures	287:306	The water-soluble fractions of pectin extracted from the pulp of ripe papayas have already been found to exert positive effects on cancer cell cultures.
32828888	0	36	theme	colon	130:134	arg1	growth					148:153	colon cancer cell growth	130:153	colon cancer cell growth	130:153	The acid and neutral fractions of pectins isolated from ripe and overripe papayas differentially affect galectin-3 inhibition and colon cancer cell growth.
32828888	7	37	theme	chemical	1380:1387	arg1	structures					1389:1398	the chemical structures	1376:1398	the chemical structures of papaya pectins	1376:1416	The results obtained may help to establish a relationship between the chemical structures of papaya pectins and the positive in vitro biological effects, such as inhibiting cancer cell growth.
32828888	2	38	theme	pectin	379:384	arg1	characteristics					386:400	the pectin characteristics	375:400	the pectin characteristics that exert these effects	375:425	However, the mechanisms that lead to these beneficial effects and the pectin characteristics that exert these effects are still not well understood.
32828888	1	39	theme	cell	294:297	arg1	cultures					299:306	cancer cell cultures	287:306	cancer cell cultures	287:306	The water-soluble fractions of pectin extracted from the pulp of ripe papayas have already been found to exert positive effects on cancer cell cultures.
32828888	7	40	dep	in	1435:1436	arg1	vitro					1438:1442	vitro	1438:1442	vitro	1438:1442	The results obtained may help to establish a relationship between the chemical structures of papaya pectins and the positive in vitro biological effects, such as inhibiting cancer cell growth.
32828888	5	41	dep	ripening	855:862	arg1	day					882:884	fourth and ninth day	865:884	fourth and ninth day after harvesting	865:901	The aims of this work are to fractionate the pectin extracted from the pulp of papayas at two stages of ripening (fourth and ninth day after harvesting) into uronic and neutral fractions and to test them for the inhibition of human recombinant galectin-3 and the inhibition of colon cancer cell growth.
32828888	0	42	theme	pectins	34:40	arg1	fractions					21:29	The acid and neutral fractions	0:29	fractions	21:29	The acid and neutral fractions of pectins isolated from ripe and overripe papayas differentially affect galectin-3 inhibition and colon cancer cell growth.
32828888	0	43	theme	cell	143:146	arg1	growth					148:153	colon cancer cell growth	130:153	colon cancer cell growth	130:153	The acid and neutral fractions of pectins isolated from ripe and overripe papayas differentially affect galectin-3 inhibition and colon cancer cell growth.
32828888	5	44	theme	colon	1028:1032	arg1	growth					1046:1051	colon cancer cell growth	1028:1051	colon cancer cell growth	1028:1051	The aims of this work are to fractionate the pectin extracted from the pulp of papayas at two stages of ripening (fourth and ninth day after harvesting) into uronic and neutral fractions and to test them for the inhibition of human recombinant galectin-3 and the inhibition of colon cancer cell growth.
32828888	3	45	theme	molecular	482:490	arg1	size					492:495	molecular size	482:495	molecular size	482:495	Characteristics such as molecular size, monosaccharide composition and structural conformation are known as polysaccharide factors that can cause alterations in cellular response.
32828888	6	46	theme	best	1202:1205	arg1	effects					1218:1224	the best biological effects	1198:1224	the best biological effects	1198:1224	The structures of the fractions were chemically characterized, and the uronic fraction extracted from the fourth day after harvesting presented the best biological effects across different concentrations in both galectin-3 inhibition and viability assays.
32828888	5	47	theme	recombinant	983:993	arg1	galectin-3					995:1004	human recombinant galectin-3	977:1004	human recombinant galectin-3	977:1004	The aims of this work are to fractionate the pectin extracted from the pulp of papayas at two stages of ripening (fourth and ninth day after harvesting) into uronic and neutral fractions and to test them for the inhibition of human recombinant galectin-3 and the inhibition of colon cancer cell growth.
32828888	4	48	theme	polysaccharide	669:682	arg1	solubilization					684:697	a major polysaccharide solubilization	661:697	a major polysaccharide solubilization	661:697	During fruit ripening, a major polysaccharide solubilization, depolymerization, and chemical modification occur.
32828888	5	49	theme	uronic	909:914	arg1	fractions					928:936	uronic and neutral fractions	909:936	uronic and neutral fractions	909:936	The aims of this work are to fractionate the pectin extracted from the pulp of papayas at two stages of ripening (fourth and ninth day after harvesting) into uronic and neutral fractions and to test them for the inhibition of human recombinant galectin-3 and the inhibition of colon cancer cell growth.
32828888	7	50	theme	papaya	1403:1408	arg1	pectins					1410:1416	papaya pectins	1403:1416	papaya pectins	1403:1416	The results obtained may help to establish a relationship between the chemical structures of papaya pectins and the positive in vitro biological effects, such as inhibiting cancer cell growth.
32828888	7	51	theme	in	1435:1436	arg1	effects					1455:1461	the positive in vitro biological effects	1422:1461	the positive in vitro biological effects	1422:1461	The results obtained may help to establish a relationship between the chemical structures of papaya pectins and the positive in vitro biological effects, such as inhibiting cancer cell growth.
32828888	5	52	theme	cancer	1034:1039	arg1	growth					1046:1051	colon cancer cell growth	1028:1051	colon cancer cell growth	1028:1051	The aims of this work are to fractionate the pectin extracted from the pulp of papayas at two stages of ripening (fourth and ninth day after harvesting) into uronic and neutral fractions and to test them for the inhibition of human recombinant galectin-3 and the inhibition of colon cancer cell growth.
32828888	1	53	theme	ripe	221:224	arg1	papayas					226:232	ripe papayas	221:232	ripe papayas	221:232	The water-soluble fractions of pectin extracted from the pulp of ripe papayas have already been found to exert positive effects on cancer cell cultures.
32828888	0	54	theme	ripe	56:59	arg1	papayas					74:80	ripe and overripe papayas	56:80	ripe and overripe papayas	56:80	The acid and neutral fractions of pectins isolated from ripe and overripe papayas differentially affect galectin-3 inhibition and colon cancer cell growth.
32828888	5	55	theme	cell	1041:1044	arg1	growth					1046:1051	colon cancer cell growth	1028:1051	colon cancer cell growth	1028:1051	The aims of this work are to fractionate the pectin extracted from the pulp of papayas at two stages of ripening (fourth and ninth day after harvesting) into uronic and neutral fractions and to test them for the inhibition of human recombinant galectin-3 and the inhibition of colon cancer cell growth.
32828888	5	56	theme	human	977:981	arg1	galectin-3					995:1004	human recombinant galectin-3	977:1004	human recombinant galectin-3	977:1004	The aims of this work are to fractionate the pectin extracted from the pulp of papayas at two stages of ripening (fourth and ninth day after harvesting) into uronic and neutral fractions and to test them for the inhibition of human recombinant galectin-3 and the inhibition of colon cancer cell growth.
32828888	3	57	theme	monosaccharide	498:511	arg1	composition					513:523	monosaccharide composition	498:523	monosaccharide composition	498:523	Characteristics such as molecular size, monosaccharide composition and structural conformation are known as polysaccharide factors that can cause alterations in cellular response.
32828888	1	58	theme	papayas	226:232	arg1	pulp					213:216	the pulp	209:216	the pulp of ripe papayas	209:232	The water-soluble fractions of pectin extracted from the pulp of ripe papayas have already been found to exert positive effects on cancer cell cultures.
32828888	7	59	theme	cell	1490:1493	arg1	growth					1495:1500	cancer cell growth	1483:1500	cancer cell growth	1483:1500	The results obtained may help to establish a relationship between the chemical structures of papaya pectins and the positive in vitro biological effects, such as inhibiting cancer cell growth.
32828888	3	60	theme	cellular	619:626	arg1	response					628:635	cellular response	619:635	cellular response	619:635	Characteristics such as molecular size, monosaccharide composition and structural conformation are known as polysaccharide factors that can cause alterations in cellular response.
32828888	6	61	theme	fractions	1076:1084	arg1	structures					1058:1067	The structures	1054:1067	The structures of the fractions	1054:1084	The structures of the fractions were chemically characterized, and the uronic fraction extracted from the fourth day after harvesting presented the best biological effects across different concentrations in both galectin-3 inhibition and viability assays.
34481389	0	0	theme	peroxydisulfate	76:90	arg1	nanotubes					35:43	carbon nanotubes	28:43	carbon nanotubes	28:43	Natural cellulose supported carbon nanotubes and Fe3O4 NPs as the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process.
34481389	0	0	theme	peroxydisulfate	76:90	arg1	activator					92:100	the efficient peroxydisulfate activator	62:100	the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process	62:174	Natural cellulose supported carbon nanotubes and Fe3O4 NPs as the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process.
34481389	0	0	theme	peroxydisulfate	76:90	arg1	NPs					55:57	Fe3O4 NPs	49:57	Fe3O4 NPs	49:57	Natural cellulose supported carbon nanotubes and Fe3O4 NPs as the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process.
34481389	4	1	theme	sheath-like	916:926	arg1	layer					939:943	the sheath-like protective layer	912:943	the sheath-like protective layer formed by urea	912:958	Interestingly, the strong interaction between CNTs and the sheath-like protective layer formed by urea on the cellulose surface promotes the introduction of nitrogen into the composites at the preparation temperature of 70 °C. Moreover, the mechanism of the system was found to be a typical non-radical pathway.
34481389	2	2	theme	green	580:584	arg1	system					600:605	a green alkaline-urea system	578:605	a green alkaline-urea system	578:605	In this study, the magnetic and porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles (RC/CNTs/Fe3O4 NPs) composites were synthesized for activation of peroxydisulfate (PDS) in a green alkaline-urea system.
34481389	0	3	theme	efficient	66:74	arg1	nanotubes					35:43	carbon nanotubes	28:43	carbon nanotubes	28:43	Natural cellulose supported carbon nanotubes and Fe3O4 NPs as the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process.
34481389	0	3	theme	efficient	66:74	arg1	activator					92:100	the efficient peroxydisulfate activator	62:100	the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process	62:174	Natural cellulose supported carbon nanotubes and Fe3O4 NPs as the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process.
34481389	0	3	theme	efficient	66:74	arg1	NPs					55:57	Fe3O4 NPs	49:57	Fe3O4 NPs	49:57	Natural cellulose supported carbon nanotubes and Fe3O4 NPs as the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process.
34481389	4	4	from	interaction	883:893	arg1	surface					977:983	the cellulose surface	963:983	the cellulose surface	963:983	Interestingly, the strong interaction between CNTs and the sheath-like protective layer formed by urea on the cellulose surface promotes the introduction of nitrogen into the composites at the preparation temperature of 70 °C. Moreover, the mechanism of the system was found to be a typical non-radical pathway.
34481389	4	5	theme	strong	876:881	arg1	interaction					883:893	the strong interaction	872:893	the strong interaction between CNTs and the sheath-like protective layer formed by urea on the cellulose surface	872:983	Interestingly, the strong interaction between CNTs and the sheath-like protective layer formed by urea on the cellulose surface promotes the introduction of nitrogen into the composites at the preparation temperature of 70 °C. Moreover, the mechanism of the system was found to be a typical non-radical pathway.
34481389	4	6	theme	non-radical	1148:1158	arg1	mechanism					1098:1106	the mechanism	1094:1106	the mechanism of the system	1094:1120	Interestingly, the strong interaction between CNTs and the sheath-like protective layer formed by urea on the cellulose surface promotes the introduction of nitrogen into the composites at the preparation temperature of 70 °C. Moreover, the mechanism of the system was found to be a typical non-radical pathway.
34481389	4	6	theme	non-radical	1148:1158	arg1	pathway					1160:1166	a typical non-radical pathway	1138:1166	a typical non-radical pathway	1138:1166	Interestingly, the strong interaction between CNTs and the sheath-like protective layer formed by urea on the cellulose surface promotes the introduction of nitrogen into the composites at the preparation temperature of 70 °C. Moreover, the mechanism of the system was found to be a typical non-radical pathway.
34481389	3	7	theme	functional	797:806	arg1	groups					808:813	functional groups	797:813	functional groups	797:813	The RC/CNTs/Fe3O4 NPs-PDS system achieved 100% removal of bisphenol A compared with CNTs (~64.6%), RC (~0%) or Fe3O4 NPs (~0%), which was closely related to the introduction of defects and functional groups, nitrogen doping and conductive networks.
34481389	1	8	theme	low	340:342	arg1	efficiency					353:362	low recovery efficiency	340:362	low recovery efficiency	340:362	Currently, many catalysts are inconvenient to separate from water, and the solvents used in the preparation process are not environmentally friendly, resulting in low recovery efficiency and secondary pollution.
34481389	2	9	theme	alkaline-urea	586:598	arg1	system					600:605	a green alkaline-urea system	578:605	a green alkaline-urea system	578:605	In this study, the magnetic and porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles (RC/CNTs/Fe3O4 NPs) composites were synthesized for activation of peroxydisulfate (PDS) in a green alkaline-urea system.
34481389	6	10	theme	composite	1411:1419	arg1	superiority					1392:1402	superiority	1392:1402	superiority	1392:1402	The recycling and separation experiments revealed the practicality and superiority of the composite.
34481389	6	10	theme	composite	1411:1419	arg1	practicality					1375:1386	practicality	1375:1386	practicality	1375:1386	The recycling and separation experiments revealed the practicality and superiority of the composite.
34481389	7	11	theme	sustainable	1456:1466	arg1	strategy					1468:1475	a feasible and sustainable strategy	1441:1475	a feasible and sustainable strategy for the application of natural cellulose-supported catalysts	1441:1536	This work provides a feasible and sustainable strategy for the application of natural cellulose-supported catalysts.
34481389	1	12	theme	recovery	344:351	arg1	efficiency					353:362	low recovery efficiency	340:362	low recovery efficiency	340:362	Currently, many catalysts are inconvenient to separate from water, and the solvents used in the preparation process are not environmentally friendly, resulting in low recovery efficiency and secondary pollution.
34481389	3	13	theme	conductive	836:845	arg1	defects					785:791	defects	785:791	defects	785:791	The RC/CNTs/Fe3O4 NPs-PDS system achieved 100% removal of bisphenol A compared with CNTs (~64.6%), RC (~0%) or Fe3O4 NPs (~0%), which was closely related to the introduction of defects and functional groups, nitrogen doping and conductive networks.
34481389	3	13	theme	conductive	836:845	arg1	networks					847:854	conductive networks	836:854	conductive networks	836:854	The RC/CNTs/Fe3O4 NPs-PDS system achieved 100% removal of bisphenol A compared with CNTs (~64.6%), RC (~0%) or Fe3O4 NPs (~0%), which was closely related to the introduction of defects and functional groups, nitrogen doping and conductive networks.
34481389	0	14	theme	Fe3O4	49:53	arg1	nanotubes					35:43	carbon nanotubes	28:43	carbon nanotubes	28:43	Natural cellulose supported carbon nanotubes and Fe3O4 NPs as the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process.
34481389	0	14	theme	Fe3O4	49:53	arg1	activator					92:100	the efficient peroxydisulfate activator	62:100	the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process	62:174	Natural cellulose supported carbon nanotubes and Fe3O4 NPs as the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process.
34481389	0	14	theme	Fe3O4	49:53	arg1	NPs					55:57	Fe3O4 NPs	49:57	Fe3O4 NPs	49:57	Natural cellulose supported carbon nanotubes and Fe3O4 NPs as the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process.
34481389	2	15	dep	magnetic	408:415	arg1	composites					507:516	composites	507:516	composites	507:516	In this study, the magnetic and porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles (RC/CNTs/Fe3O4 NPs) composites were synthesized for activation of peroxydisulfate (PDS) in a green alkaline-urea system.
34481389	5	16	theme	different	1288:1296	arg1	anions					1298:1303	different anions	1288:1303	different anions	1288:1303	Fortunately, there is no leaching of iron ions in the system, and the effects of the actual waterbody, initial pH, and different anions are negligible.
34481389	2	17	theme	porous	421:426	arg1	nanoparticles					473:485	porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles	421:485	porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles (RC/CNTs/Fe3O4 NPs)	421:505	In this study, the magnetic and porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles (RC/CNTs/Fe3O4 NPs) composites were synthesized for activation of peroxydisulfate (PDS) in a green alkaline-urea system.
34481389	2	17	theme	porous	421:426	arg1	NPs					502:504	RC/CNTs/Fe3O4 NPs	488:504	RC/CNTs/Fe3O4 NPs	488:504	In this study, the magnetic and porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles (RC/CNTs/Fe3O4 NPs) composites were synthesized for activation of peroxydisulfate (PDS) in a green alkaline-urea system.
34481389	3	18	theme	nitrogen	816:823	arg1	defects					785:791	defects	785:791	defects	785:791	The RC/CNTs/Fe3O4 NPs-PDS system achieved 100% removal of bisphenol A compared with CNTs (~64.6%), RC (~0%) or Fe3O4 NPs (~0%), which was closely related to the introduction of defects and functional groups, nitrogen doping and conductive networks.
34481389	3	18	theme	nitrogen	816:823	arg1	doping					825:830	nitrogen doping	816:830	nitrogen doping	816:830	The RC/CNTs/Fe3O4 NPs-PDS system achieved 100% removal of bisphenol A compared with CNTs (~64.6%), RC (~0%) or Fe3O4 NPs (~0%), which was closely related to the introduction of defects and functional groups, nitrogen doping and conductive networks.
34481389	7	19	theme	cellulose-supported	1508:1526	arg1	catalysts					1528:1536	natural cellulose-supported catalysts	1500:1536	natural cellulose-supported catalysts	1500:1536	This work provides a feasible and sustainable strategy for the application of natural cellulose-supported catalysts.
34481389	2	20	theme	regenerated	428:438	arg1	nanoparticles					473:485	porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles	421:485	porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles (RC/CNTs/Fe3O4 NPs)	421:505	In this study, the magnetic and porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles (RC/CNTs/Fe3O4 NPs) composites were synthesized for activation of peroxydisulfate (PDS) in a green alkaline-urea system.
34481389	2	20	theme	regenerated	428:438	arg1	NPs					502:504	RC/CNTs/Fe3O4 NPs	488:504	RC/CNTs/Fe3O4 NPs	488:504	In this study, the magnetic and porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles (RC/CNTs/Fe3O4 NPs) composites were synthesized for activation of peroxydisulfate (PDS) in a green alkaline-urea system.
34481389	2	21	theme	RC/CNTs/Fe3O4	488:500	arg1	nanoparticles					473:485	porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles	421:485	porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles (RC/CNTs/Fe3O4 NPs)	421:505	In this study, the magnetic and porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles (RC/CNTs/Fe3O4 NPs) composites were synthesized for activation of peroxydisulfate (PDS) in a green alkaline-urea system.
34481389	2	21	theme	RC/CNTs/Fe3O4	488:500	arg1	NPs					502:504	RC/CNTs/Fe3O4 NPs	488:504	RC/CNTs/Fe3O4 NPs	488:504	In this study, the magnetic and porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles (RC/CNTs/Fe3O4 NPs) composites were synthesized for activation of peroxydisulfate (PDS) in a green alkaline-urea system.
34481389	5	22	theme	anions	1298:1303	arg1	negligible					1309:1318	negligible	1309:1318	negligible	1309:1318	Fortunately, there is no leaching of iron ions in the system, and the effects of the actual waterbody, initial pH, and different anions are negligible.
34481389	5	22	theme	anions	1298:1303	arg1	effects					1239:1245	the effects	1235:1245	the effects of the actual waterbody, initial pH, and different anions	1235:1303	Fortunately, there is no leaching of iron ions in the system, and the effects of the actual waterbody, initial pH, and different anions are negligible.
34481389	4	23	theme	70 °C.	1077:1082	arg1	temperature					1062:1072	the preparation temperature	1046:1072	the preparation temperature of 70 °C.	1046:1082	Interestingly, the strong interaction between CNTs and the sheath-like protective layer formed by urea on the cellulose surface promotes the introduction of nitrogen into the composites at the preparation temperature of 70 °C. Moreover, the mechanism of the system was found to be a typical non-radical pathway.
34481389	0	24	theme	Natural	0:6	arg1	cellulose					8:16	Natural cellulose	0:16	Natural cellulose	0:16	Natural cellulose supported carbon nanotubes and Fe3O4 NPs as the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process.
34481389	4	25	theme	typical	1140:1146	arg1	mechanism					1098:1106	the mechanism	1094:1106	the mechanism of the system	1094:1120	Interestingly, the strong interaction between CNTs and the sheath-like protective layer formed by urea on the cellulose surface promotes the introduction of nitrogen into the composites at the preparation temperature of 70 °C. Moreover, the mechanism of the system was found to be a typical non-radical pathway.
34481389	4	25	theme	typical	1140:1146	arg1	pathway					1160:1166	a typical non-radical pathway	1138:1166	a typical non-radical pathway	1138:1166	Interestingly, the strong interaction between CNTs and the sheath-like protective layer formed by urea on the cellulose surface promotes the introduction of nitrogen into the composites at the preparation temperature of 70 °C. Moreover, the mechanism of the system was found to be a typical non-radical pathway.
34481389	0	26	theme	carbon	28:33	arg1	activator					92:100	the efficient peroxydisulfate activator	62:100	the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process	62:174	Natural cellulose supported carbon nanotubes and Fe3O4 NPs as the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process.
34481389	0	26	theme	carbon	28:33	arg1	nanotubes					35:43	carbon nanotubes	28:43	carbon nanotubes	28:43	Natural cellulose supported carbon nanotubes and Fe3O4 NPs as the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process.
34481389	0	26	theme	carbon	28:33	arg1	NPs					55:57	Fe3O4 NPs	49:57	Fe3O4 NPs	49:57	Natural cellulose supported carbon nanotubes and Fe3O4 NPs as the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process.
34481389	0	27	theme	bisphenol	121:129	arg1	A					131:131	bisphenol A	121:131	bisphenol A: An enhanced non-radical oxidation process	121:174	Natural cellulose supported carbon nanotubes and Fe3O4 NPs as the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process.
34481389	5	28	from	leaching	1194:1201	arg1	system					1223:1228	the system	1219:1228	the system	1219:1228	Fortunately, there is no leaching of iron ions in the system, and the effects of the actual waterbody, initial pH, and different anions are negligible.
34481389	1	29	theme	many	188:191	arg1	catalysts					193:201	many catalysts	188:201	many catalysts	188:201	Currently, many catalysts are inconvenient to separate from water, and the solvents used in the preparation process are not environmentally friendly, resulting in low recovery efficiency and secondary pollution.
34481389	6	30	dep	practicality	1375:1386	arg1	the					1371:1373	the	1371:1373	the	1371:1373	The recycling and separation experiments revealed the practicality and superiority of the composite.
34481389	1	31	theme	secondary	368:376	arg1	pollution					378:386	secondary pollution	368:386	secondary pollution	368:386	Currently, many catalysts are inconvenient to separate from water, and the solvents used in the preparation process are not environmentally friendly, resulting in low recovery efficiency and secondary pollution.
34481389	4	32	theme	preparation	1050:1060	arg1	temperature					1062:1072	the preparation temperature	1046:1072	the preparation temperature of 70 °C.	1046:1082	Interestingly, the strong interaction between CNTs and the sheath-like protective layer formed by urea on the cellulose surface promotes the introduction of nitrogen into the composites at the preparation temperature of 70 °C. Moreover, the mechanism of the system was found to be a typical non-radical pathway.
34481389	1	33	theme	preparation	273:283	arg1	process					285:291	the preparation process	269:291	the preparation process	269:291	Currently, many catalysts are inconvenient to separate from water, and the solvents used in the preparation process are not environmentally friendly, resulting in low recovery efficiency and secondary pollution.
34481389	2	34	theme	peroxydisulfate	553:567	arg1	activation					539:548	activation	539:548	activation of peroxydisulfate (PDS)	539:573	In this study, the magnetic and porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles (RC/CNTs/Fe3O4 NPs) composites were synthesized for activation of peroxydisulfate (PDS) in a green alkaline-urea system.
34481389	7	35	theme	feasible	1443:1450	arg1	strategy					1468:1475	a feasible and sustainable strategy	1441:1475	a feasible and sustainable strategy for the application of natural cellulose-supported catalysts	1441:1536	This work provides a feasible and sustainable strategy for the application of natural cellulose-supported catalysts.
34481389	4	36	theme	protective	928:937	arg1	layer					939:943	the sheath-like protective layer	912:943	the sheath-like protective layer formed by urea	912:958	Interestingly, the strong interaction between CNTs and the sheath-like protective layer formed by urea on the cellulose surface promotes the introduction of nitrogen into the composites at the preparation temperature of 70 °C. Moreover, the mechanism of the system was found to be a typical non-radical pathway.
34481389	0	37	theme	A	131:131	arg1	removal					110:116	the removal	106:116	the removal of bisphenol A: An enhanced non-radical oxidation process	106:174	Natural cellulose supported carbon nanotubes and Fe3O4 NPs as the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process.
34481389	3	38	theme	100	650:652	arg1	%					653:653	%	653:653	%	653:653	The RC/CNTs/Fe3O4 NPs-PDS system achieved 100% removal of bisphenol A compared with CNTs (~64.6%), RC (~0%) or Fe3O4 NPs (~0%), which was closely related to the introduction of defects and functional groups, nitrogen doping and conductive networks.
34481389	4	39	theme	cellulose	967:975	arg1	surface					977:983	the cellulose surface	963:983	the cellulose surface	963:983	Interestingly, the strong interaction between CNTs and the sheath-like protective layer formed by urea on the cellulose surface promotes the introduction of nitrogen into the composites at the preparation temperature of 70 °C. Moreover, the mechanism of the system was found to be a typical non-radical pathway.
34481389	4	40	theme	system	1115:1120	arg1	mechanism					1098:1106	the mechanism	1094:1106	the mechanism of the system	1094:1120	Interestingly, the strong interaction between CNTs and the sheath-like protective layer formed by urea on the cellulose surface promotes the introduction of nitrogen into the composites at the preparation temperature of 70 °C. Moreover, the mechanism of the system was found to be a typical non-radical pathway.
34481389	4	40	theme	system	1115:1120	arg1	pathway					1160:1166	a typical non-radical pathway	1138:1166	a typical non-radical pathway	1138:1166	Interestingly, the strong interaction between CNTs and the sheath-like protective layer formed by urea on the cellulose surface promotes the introduction of nitrogen into the composites at the preparation temperature of 70 °C. Moreover, the mechanism of the system was found to be a typical non-radical pathway.
34481389	3	41	dep	achieved	641:648	arg1	related					754:760	related	754:760	related	754:760	The RC/CNTs/Fe3O4 NPs-PDS system achieved 100% removal of bisphenol A compared with CNTs (~64.6%), RC (~0%) or Fe3O4 NPs (~0%), which was closely related to the introduction of defects and functional groups, nitrogen doping and conductive networks.
34481389	2	42	theme	nanotubes/Fe3O4	457:471	arg1	nanoparticles					473:485	porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles	421:485	porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles (RC/CNTs/Fe3O4 NPs)	421:505	In this study, the magnetic and porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles (RC/CNTs/Fe3O4 NPs) composites were synthesized for activation of peroxydisulfate (PDS) in a green alkaline-urea system.
34481389	2	42	theme	nanotubes/Fe3O4	457:471	arg1	NPs					502:504	RC/CNTs/Fe3O4 NPs	488:504	RC/CNTs/Fe3O4 NPs	488:504	In this study, the magnetic and porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles (RC/CNTs/Fe3O4 NPs) composites were synthesized for activation of peroxydisulfate (PDS) in a green alkaline-urea system.
34481389	0	43	theme	enhanced	137:144	arg1	process					168:174	An enhanced non-radical oxidation process	134:174	bisphenol A: An enhanced non-radical oxidation process	121:174	Natural cellulose supported carbon nanotubes and Fe3O4 NPs as the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process.
34481389	3	44	theme	%	653:653	arg1	removal					655:661	100% removal	650:661	100% removal of bisphenol A	650:676	The RC/CNTs/Fe3O4 NPs-PDS system achieved 100% removal of bisphenol A compared with CNTs (~64.6%), RC (~0%) or Fe3O4 NPs (~0%), which was closely related to the introduction of defects and functional groups, nitrogen doping and conductive networks.
34481389	5	45	theme	pH	1280:1281	arg1	negligible					1309:1318	negligible	1309:1318	negligible	1309:1318	Fortunately, there is no leaching of iron ions in the system, and the effects of the actual waterbody, initial pH, and different anions are negligible.
34481389	5	45	theme	pH	1280:1281	arg1	effects					1239:1245	the effects	1235:1245	the effects of the actual waterbody, initial pH, and different anions	1235:1303	Fortunately, there is no leaching of iron ions in the system, and the effects of the actual waterbody, initial pH, and different anions are negligible.
34481389	6	46	theme	recycling	1325:1333	arg1	experiments					1350:1360	The recycling and separation experiments	1321:1360	experiments	1350:1360	The recycling and separation experiments revealed the practicality and superiority of the composite.
34481389	2	47	theme	cellulose/carbon	440:455	arg1	nanoparticles					473:485	porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles	421:485	porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles (RC/CNTs/Fe3O4 NPs)	421:505	In this study, the magnetic and porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles (RC/CNTs/Fe3O4 NPs) composites were synthesized for activation of peroxydisulfate (PDS) in a green alkaline-urea system.
34481389	2	47	theme	cellulose/carbon	440:455	arg1	NPs					502:504	RC/CNTs/Fe3O4 NPs	488:504	RC/CNTs/Fe3O4 NPs	488:504	In this study, the magnetic and porous regenerated cellulose/carbon nanotubes/Fe3O4 nanoparticles (RC/CNTs/Fe3O4 NPs) composites were synthesized for activation of peroxydisulfate (PDS) in a green alkaline-urea system.
34481389	3	48	theme	Fe3O4	719:723	arg1	NPs					725:727	Fe3O4 NPs	719:727	Fe3O4 NPs (~0%)	719:733	The RC/CNTs/Fe3O4 NPs-PDS system achieved 100% removal of bisphenol A compared with CNTs (~64.6%), RC (~0%) or Fe3O4 NPs (~0%), which was closely related to the introduction of defects and functional groups, nitrogen doping and conductive networks.
34481389	3	48	theme	Fe3O4	719:723	arg1	%					732:732	~0%	730:732	~0%	730:732	The RC/CNTs/Fe3O4 NPs-PDS system achieved 100% removal of bisphenol A compared with CNTs (~64.6%), RC (~0%) or Fe3O4 NPs (~0%), which was closely related to the introduction of defects and functional groups, nitrogen doping and conductive networks.
34481389	1	49	from	water	237:241	arg1	separate					223:230	separate	223:230	separate	223:230	Currently, many catalysts are inconvenient to separate from water, and the solvents used in the preparation process are not environmentally friendly, resulting in low recovery efficiency and secondary pollution.
34481389	6	50	theme	separation	1339:1348	arg1	experiments					1350:1360	The recycling and separation experiments	1321:1360	experiments	1350:1360	The recycling and separation experiments revealed the practicality and superiority of the composite.
34481389	4	51	theme	nitrogen	1014:1021	arg1	introduction					998:1009	the introduction	994:1009	the introduction of nitrogen into the composites	994:1041	Interestingly, the strong interaction between CNTs and the sheath-like protective layer formed by urea on the cellulose surface promotes the introduction of nitrogen into the composites at the preparation temperature of 70 °C. Moreover, the mechanism of the system was found to be a typical non-radical pathway.
34481389	7	52	theme	catalysts	1528:1536	arg1	application					1485:1495	the application	1481:1495	the application of natural cellulose-supported catalysts	1481:1536	This work provides a feasible and sustainable strategy for the application of natural cellulose-supported catalysts.
34481389	0	53	theme	oxidation	158:166	arg1	process					168:174	An enhanced non-radical oxidation process	134:174	bisphenol A: An enhanced non-radical oxidation process	121:174	Natural cellulose supported carbon nanotubes and Fe3O4 NPs as the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process.
34481389	3	54	theme	defects	785:791	arg1	introduction					769:780	the introduction	765:780	the introduction of defects and functional groups, nitrogen doping and conductive networks	765:854	The RC/CNTs/Fe3O4 NPs-PDS system achieved 100% removal of bisphenol A compared with CNTs (~64.6%), RC (~0%) or Fe3O4 NPs (~0%), which was closely related to the introduction of defects and functional groups, nitrogen doping and conductive networks.
34481389	0	55	theme	non-radical	146:156	arg1	process					168:174	An enhanced non-radical oxidation process	134:174	bisphenol A: An enhanced non-radical oxidation process	121:174	Natural cellulose supported carbon nanotubes and Fe3O4 NPs as the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process.
34481389	3	56	theme	bisphenol	666:674	arg1	A					676:676	bisphenol A	666:676	bisphenol A	666:676	The RC/CNTs/Fe3O4 NPs-PDS system achieved 100% removal of bisphenol A compared with CNTs (~64.6%), RC (~0%) or Fe3O4 NPs (~0%), which was closely related to the introduction of defects and functional groups, nitrogen doping and conductive networks.
34481389	5	57	theme	initial	1272:1278	arg1	pH					1280:1281	initial pH	1272:1281	initial pH	1272:1281	Fortunately, there is no leaching of iron ions in the system, and the effects of the actual waterbody, initial pH, and different anions are negligible.
34481389	5	58	theme	iron	1206:1209	arg1	ions					1211:1214	iron ions	1206:1214	iron ions	1206:1214	Fortunately, there is no leaching of iron ions in the system, and the effects of the actual waterbody, initial pH, and different anions are negligible.
34481389	7	59	theme	natural	1500:1506	arg1	catalysts					1528:1536	natural cellulose-supported catalysts	1500:1536	natural cellulose-supported catalysts	1500:1536	This work provides a feasible and sustainable strategy for the application of natural cellulose-supported catalysts.
34481389	3	60	theme	A	676:676	arg1	removal					655:661	100% removal	650:661	100% removal of bisphenol A	650:676	The RC/CNTs/Fe3O4 NPs-PDS system achieved 100% removal of bisphenol A compared with CNTs (~64.6%), RC (~0%) or Fe3O4 NPs (~0%), which was closely related to the introduction of defects and functional groups, nitrogen doping and conductive networks.
34481389	3	61	theme	RC/CNTs/Fe3O4	612:624	arg1	system					634:639	The RC/CNTs/Fe3O4 NPs-PDS system	608:639	The RC/CNTs/Fe3O4 NPs-PDS system	608:639	The RC/CNTs/Fe3O4 NPs-PDS system achieved 100% removal of bisphenol A compared with CNTs (~64.6%), RC (~0%) or Fe3O4 NPs (~0%), which was closely related to the introduction of defects and functional groups, nitrogen doping and conductive networks.
34481389	5	62	theme	actual	1254:1259	arg1	waterbody					1261:1269	the actual waterbody	1250:1269	the actual waterbody	1250:1269	Fortunately, there is no leaching of iron ions in the system, and the effects of the actual waterbody, initial pH, and different anions are negligible.
34481389	5	63	theme	ions	1211:1214	arg1	leaching					1194:1201	no leaching	1191:1201	no leaching of iron ions in the system	1191:1228	Fortunately, there is no leaching of iron ions in the system, and the effects of the actual waterbody, initial pH, and different anions are negligible.
34481389	3	64	theme	NPs-PDS	626:632	arg1	system					634:639	The RC/CNTs/Fe3O4 NPs-PDS system	608:639	The RC/CNTs/Fe3O4 NPs-PDS system	608:639	The RC/CNTs/Fe3O4 NPs-PDS system achieved 100% removal of bisphenol A compared with CNTs (~64.6%), RC (~0%) or Fe3O4 NPs (~0%), which was closely related to the introduction of defects and functional groups, nitrogen doping and conductive networks.
34481389	5	65	theme	waterbody	1261:1269	arg1	negligible					1309:1318	negligible	1309:1318	negligible	1309:1318	Fortunately, there is no leaching of iron ions in the system, and the effects of the actual waterbody, initial pH, and different anions are negligible.
34481389	5	65	theme	waterbody	1261:1269	arg1	effects					1239:1245	the effects	1235:1245	the effects of the actual waterbody, initial pH, and different anions	1235:1303	Fortunately, there is no leaching of iron ions in the system, and the effects of the actual waterbody, initial pH, and different anions are negligible.
34481389	0	66	dep	A	131:131	arg1	process					168:174	An enhanced non-radical oxidation process	134:174	bisphenol A: An enhanced non-radical oxidation process	121:174	Natural cellulose supported carbon nanotubes and Fe3O4 NPs as the efficient peroxydisulfate activator for the removal of bisphenol A: An enhanced non-radical oxidation process.
34481389	3	67	theme	groups	808:813	arg1	introduction					769:780	the introduction	765:780	the introduction of defects and functional groups, nitrogen doping and conductive networks	765:854	The RC/CNTs/Fe3O4 NPs-PDS system achieved 100% removal of bisphenol A compared with CNTs (~64.6%), RC (~0%) or Fe3O4 NPs (~0%), which was closely related to the introduction of defects and functional groups, nitrogen doping and conductive networks.
34364619	2	0	theme	carbohydrate	511:522	arg1	analysis					524:531	carbohydrate analysis	511:531	carbohydrate analysis	511:531	The composition and structure of the LCCs fractions were investigated via carbohydrate analysis, XRD, FT-IR, and 2D HSQC NMR.
34364619	1	1	theme	Fenton	350:355	arg1	oxidation					357:365	Fenton oxidation	350:365	Fenton oxidation	350:365	In this study, lignin-carbohydrate complexes (LCCs) were isolated from biomass (raw and pretreated) to investigate the structural changes in biomass pretreated by Fenton oxidation and hydrothermal treatment, and their effect on enzymatic hydrolysis.
34364619	8	2	theme	hydrolysis	1388:1397	arg1	%					1452:1452	93.53%	1447:1452	93.53%	1447:1452	The efficiencies of enzymatic hydrolysis for the pretreated yellow poplar and larch were 93.53% and 26.23%, respectively.
34364619	8	2	theme	hydrolysis	1388:1397	arg1	efficiencies					1362:1373	The efficiencies	1358:1373	The efficiencies of enzymatic hydrolysis for the pretreated yellow poplar and larch	1358:1440	The efficiencies of enzymatic hydrolysis for the pretreated yellow poplar and larch were 93.53% and 26.23%, respectively.
34364619	5	3	theme	glucan-rich	875:885	arg1	LCC1					892:895	LCC1	892:895	LCC1	892:895	The fractional yield of glucan-rich LCC (LCC1) in the yellow poplar (raw and pretreated biomass) was high, while that of glucomannan-rich LCC (LCC3) in larch was higher than the yield yellow poplar.
34364619	5	3	theme	glucan-rich	875:885	arg1	LCC					887:889	glucan-rich LCC	875:889	glucan-rich LCC (LCC1)	875:896	The fractional yield of glucan-rich LCC (LCC1) in the yellow poplar (raw and pretreated biomass) was high, while that of glucomannan-rich LCC (LCC3) in larch was higher than the yield yellow poplar.
34364619	1	4	theme	hydrothermal	371:382	arg1	treatment					384:392	hydrothermal treatment	371:392	hydrothermal treatment	371:392	In this study, lignin-carbohydrate complexes (LCCs) were isolated from biomass (raw and pretreated) to investigate the structural changes in biomass pretreated by Fenton oxidation and hydrothermal treatment, and their effect on enzymatic hydrolysis.
34364619	0	5	theme	Fenton	90:95	arg1	oxidation					97:105	Fenton oxidation	90:105	Fenton oxidation	90:105	Structural characterization of the lignin-carbohydrate complex in biomass pretreated with Fenton oxidation and hydrothermal treatment and consequences on enzymatic hydrolysis efficiency.
34364619	0	6	from	characterization	11:26	arg1	biomass					66:72	biomass	66:72	biomass pretreated with Fenton oxidation and hydrothermal treatment and consequences on enzymatic hydrolysis efficiency	66:184	Structural characterization of the lignin-carbohydrate complex in biomass pretreated with Fenton oxidation and hydrothermal treatment and consequences on enzymatic hydrolysis efficiency.
34364619	5	7	theme	LCC	887:889	arg1	high					952:955	high	952:955	high	952:955	The fractional yield of glucan-rich LCC (LCC1) in the yellow poplar (raw and pretreated biomass) was high, while that of glucomannan-rich LCC (LCC3) in larch was higher than the yield yellow poplar.
34364619	5	7	theme	LCC	887:889	arg1	yield					866:870	The fractional yield	851:870	The fractional yield of glucan-rich LCC (LCC1) in the yellow poplar (raw and pretreated biomass)	851:946	The fractional yield of glucan-rich LCC (LCC1) in the yellow poplar (raw and pretreated biomass) was high, while that of glucomannan-rich LCC (LCC3) in larch was higher than the yield yellow poplar.
34364619	0	8	from	oxidation	97:105	arg1	efficiency					175:184	enzymatic hydrolysis efficiency	154:184	enzymatic hydrolysis efficiency	154:184	Structural characterization of the lignin-carbohydrate complex in biomass pretreated with Fenton oxidation and hydrothermal treatment and consequences on enzymatic hydrolysis efficiency.
34364619	0	9	theme	hydrothermal	111:122	arg1	treatment					124:132	hydrothermal treatment	111:132	hydrothermal treatment	111:132	Structural characterization of the lignin-carbohydrate complex in biomass pretreated with Fenton oxidation and hydrothermal treatment and consequences on enzymatic hydrolysis efficiency.
34364619	1	10	from	changes	317:323	arg1	biomass					328:334	biomass	328:334	biomass pretreated by Fenton oxidation and hydrothermal treatment	328:392	In this study, lignin-carbohydrate complexes (LCCs) were isolated from biomass (raw and pretreated) to investigate the structural changes in biomass pretreated by Fenton oxidation and hydrothermal treatment, and their effect on enzymatic hydrolysis.
34364619	1	10	from	changes	317:323	arg1	hydrolysis					425:434	enzymatic hydrolysis	415:434	enzymatic hydrolysis	415:434	In this study, lignin-carbohydrate complexes (LCCs) were isolated from biomass (raw and pretreated) to investigate the structural changes in biomass pretreated by Fenton oxidation and hydrothermal treatment, and their effect on enzymatic hydrolysis.
34364619	6	11	theme	yellow	1132:1137	arg1	poplar					1139:1144	yellow poplar	1132:1144	yellow poplar	1132:1144	Phenyl glycoside, γ-ester, and benzyl ether linkages were observed in the LCCs of yellow poplar, while phenyl glycoside and γ-ester were detected in those of larch.
34364619	9	12	theme	enzymatic	1607:1615	arg1	hydrolysis					1617:1626	enzymatic hydrolysis	1607:1626	enzymatic hydrolysis	1607:1626	These finding indicated that the β-β', β-5, and γ-ester linkages included in the pretreated biomass affected the efficiency of enzymatic hydrolysis.
34364619	0	13	from	biomass	66:72	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of the lignin-carbohydrate complex in biomass pretreated with Fenton oxidation and hydrothermal treatment and consequences on enzymatic hydrolysis efficiency.	0:185	Structural characterization of the lignin-carbohydrate complex in biomass pretreated with Fenton oxidation and hydrothermal treatment and consequences on enzymatic hydrolysis efficiency.
34364619	5	14	from	yield	866:870	arg1	poplar					912:917	the yellow poplar	901:917	the yellow poplar (raw and pretreated biomass)	901:946	The fractional yield of glucan-rich LCC (LCC1) in the yellow poplar (raw and pretreated biomass) was high, while that of glucomannan-rich LCC (LCC3) in larch was higher than the yield yellow poplar.
34364619	7	15	from	frequencies	1243:1253	arg1	LCCs					1288:1291	the LCCs	1284:1291	the LCCs of larch	1284:1300	Following pretreatment, the frequencies of β-β', β-5, and γ-ester in the LCCs of larch were found to be higher than in those of yellow poplar.
34364619	9	16	theme	pretreated	1561:1570	arg1	biomass					1572:1578	the pretreated biomass	1557:1578	the pretreated biomass	1557:1578	These finding indicated that the β-β', β-5, and γ-ester linkages included in the pretreated biomass affected the efficiency of enzymatic hydrolysis.
34364619	9	17	theme	hydrolysis	1617:1626	arg1	efficiency					1593:1602	the efficiency	1589:1602	the efficiency of enzymatic hydrolysis	1589:1626	These finding indicated that the β-β', β-5, and γ-ester linkages included in the pretreated biomass affected the efficiency of enzymatic hydrolysis.
34364619	9	18	theme	β-β	1513:1515	arg1	linkages					1536:1543	the β-β', β-5, and γ-ester linkages	1509:1543	the β-β', β-5, and γ-ester linkages included in the pretreated biomass	1509:1578	These finding indicated that the β-β', β-5, and γ-ester linkages included in the pretreated biomass affected the efficiency of enzymatic hydrolysis.
34364619	4	19	theme	yellow	781:786	arg1	poplar					788:793	the yellow poplar	777:793	the yellow poplar	777:793	Most of the hemicellulose was degraded during pretreatment, while xylan remained in the yellow poplar, and galactan, mannan, and xylan remained in the larch.
34364619	5	20	from	LCC	989:991	arg1	larch					1003:1007	larch	1003:1007	larch	1003:1007	The fractional yield of glucan-rich LCC (LCC1) in the yellow poplar (raw and pretreated biomass) was high, while that of glucomannan-rich LCC (LCC3) in larch was higher than the yield yellow poplar.
34364619	1	21	from	effect	405:410	arg1	biomass					328:334	biomass	328:334	biomass pretreated by Fenton oxidation and hydrothermal treatment	328:392	In this study, lignin-carbohydrate complexes (LCCs) were isolated from biomass (raw and pretreated) to investigate the structural changes in biomass pretreated by Fenton oxidation and hydrothermal treatment, and their effect on enzymatic hydrolysis.
34364619	1	21	from	effect	405:410	arg1	hydrolysis					425:434	enzymatic hydrolysis	415:434	enzymatic hydrolysis	415:434	In this study, lignin-carbohydrate complexes (LCCs) were isolated from biomass (raw and pretreated) to investigate the structural changes in biomass pretreated by Fenton oxidation and hydrothermal treatment, and their effect on enzymatic hydrolysis.
34364619	0	22	from	complex	55:61	arg1	biomass					66:72	biomass	66:72	biomass pretreated with Fenton oxidation and hydrothermal treatment and consequences on enzymatic hydrolysis efficiency	66:184	Structural characterization of the lignin-carbohydrate complex in biomass pretreated with Fenton oxidation and hydrothermal treatment and consequences on enzymatic hydrolysis efficiency.
34364619	8	23	theme	enzymatic	1378:1386	arg1	hydrolysis					1388:1397	enzymatic hydrolysis	1378:1397	enzymatic hydrolysis	1378:1397	The efficiencies of enzymatic hydrolysis for the pretreated yellow poplar and larch were 93.53% and 26.23%, respectively.
34364619	6	24	theme	benzyl	1081:1086	arg1	ether					1088:1092	benzyl ether	1081:1092	benzyl ether	1081:1092	Phenyl glycoside, γ-ester, and benzyl ether linkages were observed in the LCCs of yellow poplar, while phenyl glycoside and γ-ester were detected in those of larch.
34364619	5	25	theme	yellow	1035:1040	arg1	poplar					1042:1047	the yield yellow poplar	1025:1047	the yield yellow poplar	1025:1047	The fractional yield of glucan-rich LCC (LCC1) in the yellow poplar (raw and pretreated biomass) was high, while that of glucomannan-rich LCC (LCC3) in larch was higher than the yield yellow poplar.
34364619	6	26	theme	phenyl	1153:1158	arg1	glycoside					1160:1168	phenyl glycoside	1153:1168	phenyl glycoside	1153:1168	Phenyl glycoside, γ-ester, and benzyl ether linkages were observed in the LCCs of yellow poplar, while phenyl glycoside and γ-ester were detected in those of larch.
34364619	3	27	theme	biomass	567:573	arg1	rate					587:590	The biomass degradation rate	563:590	The biomass degradation rate of yellow poplar and larch during Fenton oxidation and hydrothermal treatment	563:668	The biomass degradation rate of yellow poplar and larch during Fenton oxidation and hydrothermal treatment was approximately 30%.
34364619	3	27	theme	biomass	567:573	arg1	%					690:690	approximately 30%	674:690	approximately 30%	674:690	The biomass degradation rate of yellow poplar and larch during Fenton oxidation and hydrothermal treatment was approximately 30%.
34364619	1	28	theme	lignin-carbohydrate	202:220	arg1	LCCs					233:236	LCCs	233:236	LCCs	233:236	In this study, lignin-carbohydrate complexes (LCCs) were isolated from biomass (raw and pretreated) to investigate the structural changes in biomass pretreated by Fenton oxidation and hydrothermal treatment, and their effect on enzymatic hydrolysis.
34364619	1	28	theme	lignin-carbohydrate	202:220	arg1	complexes					222:230	lignin-carbohydrate complexes	202:230	lignin-carbohydrate complexes (LCCs)	202:237	In this study, lignin-carbohydrate complexes (LCCs) were isolated from biomass (raw and pretreated) to investigate the structural changes in biomass pretreated by Fenton oxidation and hydrothermal treatment, and their effect on enzymatic hydrolysis.
34364619	0	29	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of the lignin-carbohydrate complex in biomass pretreated with Fenton oxidation and hydrothermal treatment and consequences on enzymatic hydrolysis efficiency.	0:185	Structural characterization of the lignin-carbohydrate complex in biomass pretreated with Fenton oxidation and hydrothermal treatment and consequences on enzymatic hydrolysis efficiency.
34364619	8	30	theme	pretreated	1407:1416	arg1	poplar					1425:1430	the pretreated yellow poplar	1403:1430	the pretreated yellow poplar	1403:1430	The efficiencies of enzymatic hydrolysis for the pretreated yellow poplar and larch were 93.53% and 26.23%, respectively.
34364619	9	31	theme	β-5	1519:1521	arg1	linkages					1536:1543	the β-β', β-5, and γ-ester linkages	1509:1543	the β-β', β-5, and γ-ester linkages included in the pretreated biomass	1509:1578	These finding indicated that the β-β', β-5, and γ-ester linkages included in the pretreated biomass affected the efficiency of enzymatic hydrolysis.
34364619	7	32	theme	yellow	1343:1348	arg1	poplar					1350:1355	yellow poplar	1343:1355	yellow poplar	1343:1355	Following pretreatment, the frequencies of β-β', β-5, and γ-ester in the LCCs of larch were found to be higher than in those of yellow poplar.
34364619	3	33	theme	degradation	575:585	arg1	rate					587:590	The biomass degradation rate	563:590	The biomass degradation rate of yellow poplar and larch during Fenton oxidation and hydrothermal treatment	563:668	The biomass degradation rate of yellow poplar and larch during Fenton oxidation and hydrothermal treatment was approximately 30%.
34364619	3	33	theme	degradation	575:585	arg1	%					690:690	approximately 30%	674:690	approximately 30%	674:690	The biomass degradation rate of yellow poplar and larch during Fenton oxidation and hydrothermal treatment was approximately 30%.
34364619	6	34	located	detected	1187:1194	arg1	those					1199:1203	those	1199:1203	those	1199:1203	Phenyl glycoside, γ-ester, and benzyl ether linkages were observed in the LCCs of yellow poplar, while phenyl glycoside and γ-ester were detected in those of larch.
34364619	6	34	located	detected	1187:1194	arg2	γ-ester					1174:1180	γ-ester	1174:1180	γ-ester	1174:1180	Phenyl glycoside, γ-ester, and benzyl ether linkages were observed in the LCCs of yellow poplar, while phenyl glycoside and γ-ester were detected in those of larch.
34364619	6	34	located	detected	1187:1194	arg2	glycoside					1160:1168	phenyl glycoside	1153:1168	phenyl glycoside	1153:1168	Phenyl glycoside, γ-ester, and benzyl ether linkages were observed in the LCCs of yellow poplar, while phenyl glycoside and γ-ester were detected in those of larch.
34364619	3	35	theme	hydrothermal	647:658	arg1	treatment					660:668	hydrothermal treatment	647:668	hydrothermal treatment	647:668	The biomass degradation rate of yellow poplar and larch during Fenton oxidation and hydrothermal treatment was approximately 30%.
34364619	2	36	theme	2D	550:551	arg1	NMR					558:560	2D HSQC NMR	550:560	2D HSQC NMR	550:560	The composition and structure of the LCCs fractions were investigated via carbohydrate analysis, XRD, FT-IR, and 2D HSQC NMR.
34364619	7	37	theme	β-5	1264:1266	arg1	higher					1319:1324	higher	1319:1324	higher	1319:1324	Following pretreatment, the frequencies of β-β', β-5, and γ-ester in the LCCs of larch were found to be higher than in those of yellow poplar.
34364619	7	37	theme	β-5	1264:1266	arg1	frequencies					1243:1253	the frequencies	1239:1253	the frequencies of β-β', β-5, and γ-ester in the LCCs of larch	1239:1300	Following pretreatment, the frequencies of β-β', β-5, and γ-ester in the LCCs of larch were found to be higher than in those of yellow poplar.
34364619	6	38	theme	γ-ester	1068:1074	arg1	linkages					1094:1101	Phenyl glycoside, γ-ester, and benzyl ether linkages	1050:1101	Phenyl glycoside, γ-ester, and benzyl ether linkages	1050:1101	Phenyl glycoside, γ-ester, and benzyl ether linkages were observed in the LCCs of yellow poplar, while phenyl glycoside and γ-ester were detected in those of larch.
34364619	5	39	theme	yellow	905:910	arg1	poplar					912:917	the yellow poplar	901:917	the yellow poplar (raw and pretreated biomass)	901:946	The fractional yield of glucan-rich LCC (LCC1) in the yellow poplar (raw and pretreated biomass) was high, while that of glucomannan-rich LCC (LCC3) in larch was higher than the yield yellow poplar.
34364619	6	40	located	observed	1108:1115	arg2	linkages					1094:1101	Phenyl glycoside, γ-ester, and benzyl ether linkages	1050:1101	Phenyl glycoside, γ-ester, and benzyl ether linkages	1050:1101	Phenyl glycoside, γ-ester, and benzyl ether linkages were observed in the LCCs of yellow poplar, while phenyl glycoside and γ-ester were detected in those of larch.
34364619	6	40	located	observed	1108:1115	arg1	LCCs					1124:1127	the LCCs	1120:1127	the LCCs of yellow poplar	1120:1144	Phenyl glycoside, γ-ester, and benzyl ether linkages were observed in the LCCs of yellow poplar, while phenyl glycoside and γ-ester were detected in those of larch.
34364619	2	41	theme	HSQC	553:556	arg1	NMR					558:560	2D HSQC NMR	550:560	2D HSQC NMR	550:560	The composition and structure of the LCCs fractions were investigated via carbohydrate analysis, XRD, FT-IR, and 2D HSQC NMR.
34364619	1	42	dep	biomass	258:264	arg1	pretreated					275:284	pretreated	275:284	pretreated	275:284	In this study, lignin-carbohydrate complexes (LCCs) were isolated from biomass (raw and pretreated) to investigate the structural changes in biomass pretreated by Fenton oxidation and hydrothermal treatment, and their effect on enzymatic hydrolysis.
34364619	1	42	dep	biomass	258:264	arg1	raw					267:269	raw	267:269	raw	267:269	In this study, lignin-carbohydrate complexes (LCCs) were isolated from biomass (raw and pretreated) to investigate the structural changes in biomass pretreated by Fenton oxidation and hydrothermal treatment, and their effect on enzymatic hydrolysis.
34364619	0	43	theme	complex	55:61	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of the lignin-carbohydrate complex in biomass pretreated with Fenton oxidation and hydrothermal treatment and consequences on enzymatic hydrolysis efficiency.	0:185	Structural characterization of the lignin-carbohydrate complex in biomass pretreated with Fenton oxidation and hydrothermal treatment and consequences on enzymatic hydrolysis efficiency.
34364619	1	44	theme	structural	306:315	arg1	changes					317:323	the structural changes	302:323	the structural changes in biomass pretreated by Fenton oxidation and hydrothermal treatment	302:392	In this study, lignin-carbohydrate complexes (LCCs) were isolated from biomass (raw and pretreated) to investigate the structural changes in biomass pretreated by Fenton oxidation and hydrothermal treatment, and their effect on enzymatic hydrolysis.
34364619	0	45	theme	hydrolysis	164:173	arg1	efficiency					175:184	enzymatic hydrolysis efficiency	154:184	enzymatic hydrolysis efficiency	154:184	Structural characterization of the lignin-carbohydrate complex in biomass pretreated with Fenton oxidation and hydrothermal treatment and consequences on enzymatic hydrolysis efficiency.
34364619	3	46	theme	yellow	595:600	arg1	poplar					602:607	yellow poplar	595:607	yellow poplar	595:607	The biomass degradation rate of yellow poplar and larch during Fenton oxidation and hydrothermal treatment was approximately 30%.
34364619	5	47	theme	fractional	855:864	arg1	high					952:955	high	952:955	high	952:955	The fractional yield of glucan-rich LCC (LCC1) in the yellow poplar (raw and pretreated biomass) was high, while that of glucomannan-rich LCC (LCC3) in larch was higher than the yield yellow poplar.
34364619	5	47	theme	fractional	855:864	arg1	yield					866:870	The fractional yield	851:870	The fractional yield of glucan-rich LCC (LCC1) in the yellow poplar (raw and pretreated biomass)	851:946	The fractional yield of glucan-rich LCC (LCC1) in the yellow poplar (raw and pretreated biomass) was high, while that of glucomannan-rich LCC (LCC3) in larch was higher than the yield yellow poplar.
34364619	0	48	theme	lignin-carbohydrate	35:53	arg1	complex					55:61	the lignin-carbohydrate complex	31:61	the lignin-carbohydrate complex in biomass pretreated with Fenton oxidation and hydrothermal treatment and consequences on enzymatic hydrolysis efficiency	31:184	Structural characterization of the lignin-carbohydrate complex in biomass pretreated with Fenton oxidation and hydrothermal treatment and consequences on enzymatic hydrolysis efficiency.
34364619	0	49	theme	enzymatic	154:162	arg1	efficiency					175:184	enzymatic hydrolysis efficiency	154:184	enzymatic hydrolysis efficiency	154:184	Structural characterization of the lignin-carbohydrate complex in biomass pretreated with Fenton oxidation and hydrothermal treatment and consequences on enzymatic hydrolysis efficiency.
34364619	1	50	attach	isolated	244:251	arg1	biomass					258:264	biomass	258:264	biomass (raw and pretreated)	258:285	In this study, lignin-carbohydrate complexes (LCCs) were isolated from biomass (raw and pretreated) to investigate the structural changes in biomass pretreated by Fenton oxidation and hydrothermal treatment, and their effect on enzymatic hydrolysis.
34364619	1	50	attach	isolated	244:251	arg2	LCCs					233:236	LCCs	233:236	LCCs	233:236	In this study, lignin-carbohydrate complexes (LCCs) were isolated from biomass (raw and pretreated) to investigate the structural changes in biomass pretreated by Fenton oxidation and hydrothermal treatment, and their effect on enzymatic hydrolysis.
34364619	1	50	attach	isolated	244:251	arg2	complexes					222:230	lignin-carbohydrate complexes	202:230	lignin-carbohydrate complexes (LCCs)	202:237	In this study, lignin-carbohydrate complexes (LCCs) were isolated from biomass (raw and pretreated) to investigate the structural changes in biomass pretreated by Fenton oxidation and hydrothermal treatment, and their effect on enzymatic hydrolysis.
34364619	2	51	theme	fractions	479:487	arg1	structure					457:465	structure	457:465	structure	457:465	The composition and structure of the LCCs fractions were investigated via carbohydrate analysis, XRD, FT-IR, and 2D HSQC NMR.
34364619	2	51	theme	fractions	479:487	arg1	composition					441:451	composition	441:451	composition	441:451	The composition and structure of the LCCs fractions were investigated via carbohydrate analysis, XRD, FT-IR, and 2D HSQC NMR.
34364619	1	52	theme	enzymatic	415:423	arg1	hydrolysis					425:434	enzymatic hydrolysis	415:434	enzymatic hydrolysis	415:434	In this study, lignin-carbohydrate complexes (LCCs) were isolated from biomass (raw and pretreated) to investigate the structural changes in biomass pretreated by Fenton oxidation and hydrothermal treatment, and their effect on enzymatic hydrolysis.
34364619	2	53	dep	composition	441:451	arg1	The					437:439	The	437:439	The	437:439	The composition and structure of the LCCs fractions were investigated via carbohydrate analysis, XRD, FT-IR, and 2D HSQC NMR.
34364619	5	54	theme	yield	1029:1033	arg1	poplar					1042:1047	the yield yellow poplar	1025:1047	the yield yellow poplar	1025:1047	The fractional yield of glucan-rich LCC (LCC1) in the yellow poplar (raw and pretreated biomass) was high, while that of glucomannan-rich LCC (LCC3) in larch was higher than the yield yellow poplar.
34364619	6	55	theme	glycoside	1057:1065	arg1	linkages					1094:1101	Phenyl glycoside, γ-ester, and benzyl ether linkages	1050:1101	Phenyl glycoside, γ-ester, and benzyl ether linkages	1050:1101	Phenyl glycoside, γ-ester, and benzyl ether linkages were observed in the LCCs of yellow poplar, while phenyl glycoside and γ-ester were detected in those of larch.
34364619	2	56	theme	LCCs	474:477	arg1	fractions					479:487	the LCCs fractions	470:487	the LCCs fractions	470:487	The composition and structure of the LCCs fractions were investigated via carbohydrate analysis, XRD, FT-IR, and 2D HSQC NMR.
34364619	6	57	theme	poplar	1139:1144	arg1	LCCs					1124:1127	the LCCs	1120:1127	the LCCs of yellow poplar	1120:1144	Phenyl glycoside, γ-ester, and benzyl ether linkages were observed in the LCCs of yellow poplar, while phenyl glycoside and γ-ester were detected in those of larch.
34364619	8	58	theme	yellow	1418:1423	arg1	poplar					1425:1430	the pretreated yellow poplar	1403:1430	the pretreated yellow poplar	1403:1430	The efficiencies of enzymatic hydrolysis for the pretreated yellow poplar and larch were 93.53% and 26.23%, respectively.
34364619	5	59	theme	glucomannan-rich	972:987	arg1	LCC3					994:997	LCC3	994:997	LCC3	994:997	The fractional yield of glucan-rich LCC (LCC1) in the yellow poplar (raw and pretreated biomass) was high, while that of glucomannan-rich LCC (LCC3) in larch was higher than the yield yellow poplar.
34364619	5	59	theme	glucomannan-rich	972:987	arg1	LCC					989:991	glucomannan-rich LCC	972:991	glucomannan-rich LCC (LCC3) in larch	972:1007	The fractional yield of glucan-rich LCC (LCC1) in the yellow poplar (raw and pretreated biomass) was high, while that of glucomannan-rich LCC (LCC3) in larch was higher than the yield yellow poplar.
34364619	6	60	theme	Phenyl	1050:1055	arg1	glycoside					1057:1065	Phenyl glycoside	1050:1065	Phenyl glycoside	1050:1065	Phenyl glycoside, γ-ester, and benzyl ether linkages were observed in the LCCs of yellow poplar, while phenyl glycoside and γ-ester were detected in those of larch.
34364619	0	61	from	treatment	124:132	arg1	efficiency					175:184	enzymatic hydrolysis efficiency	154:184	enzymatic hydrolysis efficiency	154:184	Structural characterization of the lignin-carbohydrate complex in biomass pretreated with Fenton oxidation and hydrothermal treatment and consequences on enzymatic hydrolysis efficiency.
34364619	7	62	theme	β-β	1258:1260	arg1	higher					1319:1324	higher	1319:1324	higher	1319:1324	Following pretreatment, the frequencies of β-β', β-5, and γ-ester in the LCCs of larch were found to be higher than in those of yellow poplar.
34364619	7	62	theme	β-β	1258:1260	arg1	frequencies					1243:1253	the frequencies	1239:1253	the frequencies of β-β', β-5, and γ-ester in the LCCs of larch	1239:1300	Following pretreatment, the frequencies of β-β', β-5, and γ-ester in the LCCs of larch were found to be higher than in those of yellow poplar.
34364619	3	63	theme	poplar	602:607	arg1	rate					587:590	The biomass degradation rate	563:590	The biomass degradation rate of yellow poplar and larch during Fenton oxidation and hydrothermal treatment	563:668	The biomass degradation rate of yellow poplar and larch during Fenton oxidation and hydrothermal treatment was approximately 30%.
34364619	3	63	theme	poplar	602:607	arg1	%					690:690	approximately 30%	674:690	approximately 30%	674:690	The biomass degradation rate of yellow poplar and larch during Fenton oxidation and hydrothermal treatment was approximately 30%.
34364619	7	64	theme	γ-ester	1273:1279	arg1	higher					1319:1324	higher	1319:1324	higher	1319:1324	Following pretreatment, the frequencies of β-β', β-5, and γ-ester in the LCCs of larch were found to be higher than in those of yellow poplar.
34364619	7	64	theme	γ-ester	1273:1279	arg1	frequencies					1243:1253	the frequencies	1239:1253	the frequencies of β-β', β-5, and γ-ester in the LCCs of larch	1239:1300	Following pretreatment, the frequencies of β-β', β-5, and γ-ester in the LCCs of larch were found to be higher than in those of yellow poplar.
34364619	7	65	theme	larch	1296:1300	arg1	LCCs					1288:1291	the LCCs	1284:1291	the LCCs of larch	1284:1300	Following pretreatment, the frequencies of β-β', β-5, and γ-ester in the LCCs of larch were found to be higher than in those of yellow poplar.
34364619	3	66	theme	Fenton	626:631	arg1	oxidation					633:641	Fenton oxidation	626:641	Fenton oxidation	626:641	The biomass degradation rate of yellow poplar and larch during Fenton oxidation and hydrothermal treatment was approximately 30%.
34364619	5	67	theme	pretreated	928:937	arg1	biomass					939:945	pretreated biomass	928:945	pretreated biomass	928:945	The fractional yield of glucan-rich LCC (LCC1) in the yellow poplar (raw and pretreated biomass) was high, while that of glucomannan-rich LCC (LCC3) in larch was higher than the yield yellow poplar.
34364619	5	68	dep	poplar	912:917	arg1	raw					920:922	raw	920:922	raw	920:922	The fractional yield of glucan-rich LCC (LCC1) in the yellow poplar (raw and pretreated biomass) was high, while that of glucomannan-rich LCC (LCC3) in larch was higher than the yield yellow poplar.
34364619	5	68	dep	poplar	912:917	arg1	biomass					939:945	pretreated biomass	928:945	pretreated biomass	928:945	The fractional yield of glucan-rich LCC (LCC1) in the yellow poplar (raw and pretreated biomass) was high, while that of glucomannan-rich LCC (LCC3) in larch was higher than the yield yellow poplar.
34364619	6	69	theme	ether	1088:1092	arg1	linkages					1094:1101	Phenyl glycoside, γ-ester, and benzyl ether linkages	1050:1101	Phenyl glycoside, γ-ester, and benzyl ether linkages	1050:1101	Phenyl glycoside, γ-ester, and benzyl ether linkages were observed in the LCCs of yellow poplar, while phenyl glycoside and γ-ester were detected in those of larch.
34364619	3	70	theme	larch	613:617	arg1	rate					587:590	The biomass degradation rate	563:590	The biomass degradation rate of yellow poplar and larch during Fenton oxidation and hydrothermal treatment	563:668	The biomass degradation rate of yellow poplar and larch during Fenton oxidation and hydrothermal treatment was approximately 30%.
34364619	3	70	theme	larch	613:617	arg1	%					690:690	approximately 30%	674:690	approximately 30%	674:690	The biomass degradation rate of yellow poplar and larch during Fenton oxidation and hydrothermal treatment was approximately 30%.
34364619	9	71	theme	γ-ester	1528:1534	arg1	linkages					1536:1543	the β-β', β-5, and γ-ester linkages	1509:1543	the β-β', β-5, and γ-ester linkages included in the pretreated biomass	1509:1578	These finding indicated that the β-β', β-5, and γ-ester linkages included in the pretreated biomass affected the efficiency of enzymatic hydrolysis.
34364619	0	72	from	consequences	138:149	arg1	efficiency					175:184	enzymatic hydrolysis efficiency	154:184	enzymatic hydrolysis efficiency	154:184	Structural characterization of the lignin-carbohydrate complex in biomass pretreated with Fenton oxidation and hydrothermal treatment and consequences on enzymatic hydrolysis efficiency.
34600701	6	0	theme	beneficial	1203:1212	arg1	Prevotella					1244:1253	Prevotella	1244:1253	Prevotella	1244:1253	Notably, OPSI could regulate the composition and diversity of the microbial community, especially increase the abundance of beneficial bacteria such as Bacteroides, Prevotella, and Faecalibacterium.
34600701	6	0	theme	beneficial	1203:1212	arg1	bacteria					1214:1221	beneficial bacteria	1203:1221	beneficial bacteria such as Bacteroides, Prevotella, and Faecalibacterium	1203:1275	Notably, OPSI could regulate the composition and diversity of the microbial community, especially increase the abundance of beneficial bacteria such as Bacteroides, Prevotella, and Faecalibacterium.
34600701	6	0	theme	beneficial	1203:1212	arg1	Bacteroides					1231:1241	Bacteroides	1231:1241	Bacteroides	1231:1241	Notably, OPSI could regulate the composition and diversity of the microbial community, especially increase the abundance of beneficial bacteria such as Bacteroides, Prevotella, and Faecalibacterium.
34600701	6	0	theme	beneficial	1203:1212	arg1	Faecalibacterium					1260:1275	Faecalibacterium	1260:1275	Faecalibacterium	1260:1275	Notably, OPSI could regulate the composition and diversity of the microbial community, especially increase the abundance of beneficial bacteria such as Bacteroides, Prevotella, and Faecalibacterium.
34600701	5	1	used	utilized	1051:1058	arg2	OPSI					1016:1019	OPSI	1016:1019	OPSI	1016:1019	During fermentation, the Mw and carbohydrate residue of indigestible OPS (OPSI) decreased, and free monosaccharides were released, suggesting that OPSI could be further degraded and utilized by gut microbiota.
34600701	3	2	theme	OPS	460:462	arg1	effect					472:477	its effect	468:477	its effect on gut microbiota	468:495	In the present study, the digestion and fermentation properties of OPS and its effect on gut microbiota were evaluated using simulated digestion (saliva, stomach, and small intestine) and fermentation models in vitro.
34600701	3	2	theme	OPS	460:462	arg1	properties					446:455	the digestion and fermentation properties	415:455	properties	446:455	In the present study, the digestion and fermentation properties of OPS and its effect on gut microbiota were evaluated using simulated digestion (saliva, stomach, and small intestine) and fermentation models in vitro.
34600701	3	3	dep	in	601:602	arg1	vitro					604:608	vitro	604:608	vitro	604:608	In the present study, the digestion and fermentation properties of OPS and its effect on gut microbiota were evaluated using simulated digestion (saliva, stomach, and small intestine) and fermentation models in vitro.
34600701	3	4	theme	gut	482:484	arg1	microbiota					486:495	gut microbiota	482:495	gut microbiota	482:495	In the present study, the digestion and fermentation properties of OPS and its effect on gut microbiota were evaluated using simulated digestion (saliva, stomach, and small intestine) and fermentation models in vitro.
34600701	8	5	theme	potential	1574:1582	arg1	OPS					1559:1561	OPS	1559:1561	OPS	1559:1561	These results provided a reference for the digestive characteristics of OPS and revealed that OPS might be a potential prebiotic to prevent diseases by improving intestinal health.
34600701	8	5	theme	potential	1574:1582	arg1	prebiotic					1584:1592	a potential prebiotic	1572:1592	a potential prebiotic to prevent diseases by improving intestinal health	1572:1643	These results provided a reference for the digestive characteristics of OPS and revealed that OPS might be a potential prebiotic to prevent diseases by improving intestinal health.
34600701	0	6	theme	Crassostrea	93:103	arg1	oyster					85:90	oyster	85:90	oyster (Crassostrea gigas)	85:110	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	0	6	theme	Crassostrea	93:103	arg1	gigas					105:109	Crassostrea gigas	93:109	Crassostrea gigas	93:109	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	7	7	theme	fatty	1365:1369	arg1	SCFAs					1378:1382	SCFAs	1378:1382	SCFAs	1378:1382	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
34600701	7	7	theme	fatty	1365:1369	arg1	acids					1371:1375	short-chain fatty acids	1353:1375	short-chain fatty acids (SCFAs)	1353:1383	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
34600701	5	8	theme	Mw	894:895	arg1	residue					914:920	the Mw and carbohydrate residue	890:920	residue	914:920	During fermentation, the Mw and carbohydrate residue of indigestible OPS (OPSI) decreased, and free monosaccharides were released, suggesting that OPSI could be further degraded and utilized by gut microbiota.
34600701	5	8	theme	Mw	894:895	arg1	OPS					938:940	indigestible OPS	925:940	indigestible OPS (OPSI)	925:947	During fermentation, the Mw and carbohydrate residue of indigestible OPS (OPSI) decreased, and free monosaccharides were released, suggesting that OPSI could be further degraded and utilized by gut microbiota.
34600701	7	9	theme	main	1447:1450	arg1	metabolites					1452:1462	the main metabolites	1443:1462	the main metabolites	1443:1462	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
34600701	7	9	theme	main	1447:1450	arg1	acid					1413:1416	propionic acid	1403:1416	propionic acid	1403:1416	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
34600701	7	9	theme	main	1447:1450	arg1	acid					1433:1436	n-butyric acid	1423:1436	n-butyric acid	1423:1436	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
34600701	7	9	theme	main	1447:1450	arg1	acid					1397:1400	acetic acid	1390:1400	acetic acid	1390:1400	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
34600701	0	10	from	characteristics	46:60	arg1	oyster					85:90	oyster	85:90	oyster (Crassostrea gigas)	85:110	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	0	10	from	characteristics	46:60	arg1	gigas					105:109	Crassostrea gigas	93:109	Crassostrea gigas	93:109	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	0	10	from	characteristics	46:60	arg1	microbiota					140:149	the gut microbiota	132:149	the gut microbiota	132:149	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	3	11	theme	present	400:406	arg1	study					408:412	the present study	396:412	the present study	396:412	In the present study, the digestion and fermentation properties of OPS and its effect on gut microbiota were evaluated using simulated digestion (saliva, stomach, and small intestine) and fermentation models in vitro.
34600701	8	12	theme	OPS	1537:1539	arg1	characteristics					1518:1532	the digestive characteristics	1504:1532	the digestive characteristics of OPS	1504:1539	These results provided a reference for the digestive characteristics of OPS and revealed that OPS might be a potential prebiotic to prevent diseases by improving intestinal health.
34600701	7	13	theme	acids	1371:1375	arg1	production					1339:1348	the production	1335:1348	the production of short-chain fatty acids (SCFAs)	1335:1383	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
34600701	5	14	theme	carbohydrate	901:912	arg1	residue					914:920	the Mw and carbohydrate residue	890:920	residue	914:920	During fermentation, the Mw and carbohydrate residue of indigestible OPS (OPSI) decreased, and free monosaccharides were released, suggesting that OPSI could be further degraded and utilized by gut microbiota.
34600701	5	14	theme	carbohydrate	901:912	arg1	OPS					938:940	indigestible OPS	925:940	indigestible OPS (OPSI)	925:947	During fermentation, the Mw and carbohydrate residue of indigestible OPS (OPSI) decreased, and free monosaccharides were released, suggesting that OPSI could be further degraded and utilized by gut microbiota.
34600701	1	15	theme	shellfish	183:191	arg1	Oyster					152:157	Oyster	152:157	Oyster	152:157	Oyster is a kind of nutritious shellfish widely consumed globally, and its polysaccharide (OPS) has been verified to have strong functional activity.
34600701	1	15	theme	shellfish	183:191	arg1	kind					164:167	a kind	162:167	a kind of nutritious shellfish widely consumed globally	162:216	Oyster is a kind of nutritious shellfish widely consumed globally, and its polysaccharide (OPS) has been verified to have strong functional activity.
34600701	2	16	theme	specific	341:348	arg1	digestion					350:358	specific digestion	341:358	specific digestion	341:358	However, it is still unclear about the specific digestion and prebiotic properties of OPS.
34600701	0	17	theme	In	0:1	arg1	digestion					19:27	In vitro simulated digestion	0:27	In vitro simulated digestion	0:27	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	7	18	theme	short-chain	1353:1363	arg1	SCFAs					1378:1382	SCFAs	1378:1382	SCFAs	1378:1382	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
34600701	7	18	theme	short-chain	1353:1363	arg1	acids					1371:1375	short-chain fatty acids	1353:1375	short-chain fatty acids (SCFAs)	1353:1383	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
34600701	6	19	theme	community	1155:1163	arg1	composition					1112:1122	composition	1112:1122	composition	1112:1122	Notably, OPSI could regulate the composition and diversity of the microbial community, especially increase the abundance of beneficial bacteria such as Bacteroides, Prevotella, and Faecalibacterium.
34600701	6	19	theme	community	1155:1163	arg1	diversity					1128:1136	diversity	1128:1136	diversity	1128:1136	Notably, OPSI could regulate the composition and diversity of the microbial community, especially increase the abundance of beneficial bacteria such as Bacteroides, Prevotella, and Faecalibacterium.
34600701	3	20	theme	simulated	518:526	arg1	digestion					528:536	simulated digestion	518:536	simulated digestion (saliva, stomach, and small intestine)	518:575	In the present study, the digestion and fermentation properties of OPS and its effect on gut microbiota were evaluated using simulated digestion (saliva, stomach, and small intestine) and fermentation models in vitro.
34600701	8	21	theme	intestinal	1627:1636	arg1	health					1638:1643	intestinal health	1627:1643	intestinal health	1627:1643	These results provided a reference for the digestive characteristics of OPS and revealed that OPS might be a potential prebiotic to prevent diseases by improving intestinal health.
34600701	0	22	from	oyster	85:90	arg1	effects					121:127	its effects	117:127	its effects on the gut microbiota	117:149	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	0	22	from	oyster	85:90	arg1	polysaccharide					65:78	polysaccharide	65:78	polysaccharide from oyster (Crassostrea gigas)	65:110	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	0	22	from	oyster	85:90	arg1	digestion					19:27	In vitro simulated digestion	0:27	In vitro simulated digestion	0:27	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	0	22	from	oyster	85:90	arg1	characteristics					46:60	fermentation characteristics	33:60	fermentation characteristics	33:60	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	5	23	theme	indigestible	925:936	arg1	OPSI					943:946	OPSI	943:946	OPSI	943:946	During fermentation, the Mw and carbohydrate residue of indigestible OPS (OPSI) decreased, and free monosaccharides were released, suggesting that OPSI could be further degraded and utilized by gut microbiota.
34600701	5	23	theme	indigestible	925:936	arg1	OPS					938:940	indigestible OPS	925:940	indigestible OPS (OPSI)	925:947	During fermentation, the Mw and carbohydrate residue of indigestible OPS (OPSI) decreased, and free monosaccharides were released, suggesting that OPSI could be further degraded and utilized by gut microbiota.
34600701	3	24	from	properties	446:455	arg1	microbiota					486:495	gut microbiota	482:495	gut microbiota	482:495	In the present study, the digestion and fermentation properties of OPS and its effect on gut microbiota were evaluated using simulated digestion (saliva, stomach, and small intestine) and fermentation models in vitro.
34600701	1	25	contain	have	269:272	arg1	polysaccharide					227:240	its polysaccharide	223:240	its polysaccharide (OPS)	223:246	Oyster is a kind of nutritious shellfish widely consumed globally, and its polysaccharide (OPS) has been verified to have strong functional activity.
34600701	1	25	contain	have	269:272	arg1	OPS					243:245	OPS	243:245	OPS	243:245	Oyster is a kind of nutritious shellfish widely consumed globally, and its polysaccharide (OPS) has been verified to have strong functional activity.
34600701	1	25	contain	have	269:272	arg2	activity					292:299	strong functional activity	274:299	strong functional activity	274:299	Oyster is a kind of nutritious shellfish widely consumed globally, and its polysaccharide (OPS) has been verified to have strong functional activity.
34600701	6	26	theme	microbial	1145:1153	arg1	community					1155:1163	the microbial community	1141:1163	the microbial community	1141:1163	Notably, OPSI could regulate the composition and diversity of the microbial community, especially increase the abundance of beneficial bacteria such as Bacteroides, Prevotella, and Faecalibacterium.
34600701	2	27	theme	OPS	388:390	arg1	digestion					350:358	specific digestion	341:358	specific digestion	341:358	However, it is still unclear about the specific digestion and prebiotic properties of OPS.
34600701	2	27	theme	OPS	388:390	arg1	properties					374:383	prebiotic properties	364:383	prebiotic properties	364:383	However, it is still unclear about the specific digestion and prebiotic properties of OPS.
34600701	1	28	theme	nutritious	172:181	arg1	shellfish					183:191	nutritious shellfish	172:191	nutritious shellfish widely consumed globally	172:216	Oyster is a kind of nutritious shellfish widely consumed globally, and its polysaccharide (OPS) has been verified to have strong functional activity.
34600701	0	29	theme	fermentation	33:44	arg1	characteristics					46:60	fermentation characteristics	33:60	fermentation characteristics	33:60	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	5	30	theme	OPS	938:940	arg1	residue					914:920	the Mw and carbohydrate residue	890:920	residue	914:920	During fermentation, the Mw and carbohydrate residue of indigestible OPS (OPSI) decreased, and free monosaccharides were released, suggesting that OPSI could be further degraded and utilized by gut microbiota.
34600701	5	30	theme	OPS	938:940	arg1	OPS					938:940	indigestible OPS	925:940	indigestible OPS (OPSI)	925:947	During fermentation, the Mw and carbohydrate residue of indigestible OPS (OPSI) decreased, and free monosaccharides were released, suggesting that OPSI could be further degraded and utilized by gut microbiota.
34600701	3	31	theme	fermentation	581:592	arg1	models					594:599	fermentation models	581:599	fermentation models in vitro	581:608	In the present study, the digestion and fermentation properties of OPS and its effect on gut microbiota were evaluated using simulated digestion (saliva, stomach, and small intestine) and fermentation models in vitro.
34600701	4	32	theme	reducing	720:727	arg1	content					735:741	the reducing sugar content	716:741	the reducing sugar content	716:741	The results showed that the molecular weight (Mw) of OPS decreased from 5.73 × 106 to 4.35 × 106 Da, and the reducing sugar content increased from 0.043 to 0.096 mg/mL, indicating that OPS was partially degraded during the saliva-gastrointestinal digestion.
34600701	4	33	dep	4.35 × 106	697:706	arg1	to					694:695	to	694:695	to	694:695	The results showed that the molecular weight (Mw) of OPS decreased from 5.73 × 106 to 4.35 × 106 Da, and the reducing sugar content increased from 0.043 to 0.096 mg/mL, indicating that OPS was partially degraded during the saliva-gastrointestinal digestion.
34600701	2	34	theme	prebiotic	364:372	arg1	properties					374:383	prebiotic properties	364:383	prebiotic properties	364:383	However, it is still unclear about the specific digestion and prebiotic properties of OPS.
34600701	0	35	theme	simulated	9:17	arg1	digestion					19:27	In vitro simulated digestion	0:27	In vitro simulated digestion	0:27	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	4	36	theme	saliva-gastrointestinal	834:856	arg1	digestion					858:866	the saliva-gastrointestinal digestion	830:866	the saliva-gastrointestinal digestion	830:866	The results showed that the molecular weight (Mw) of OPS decreased from 5.73 × 106 to 4.35 × 106 Da, and the reducing sugar content increased from 0.043 to 0.096 mg/mL, indicating that OPS was partially degraded during the saliva-gastrointestinal digestion.
34600701	5	37	theme	gut	1063:1065	arg1	microbiota					1067:1076	gut microbiota	1063:1076	gut microbiota	1063:1076	During fermentation, the Mw and carbohydrate residue of indigestible OPS (OPSI) decreased, and free monosaccharides were released, suggesting that OPSI could be further degraded and utilized by gut microbiota.
34600701	0	38	theme	gut	136:138	arg1	microbiota					140:149	the gut microbiota	132:149	the gut microbiota	132:149	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	7	39	theme	n-butyric	1423:1431	arg1	metabolites					1452:1462	the main metabolites	1443:1462	the main metabolites	1443:1462	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
34600701	7	39	theme	n-butyric	1423:1431	arg1	acid					1413:1416	propionic acid	1403:1416	propionic acid	1403:1416	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
34600701	7	39	theme	n-butyric	1423:1431	arg1	acid					1433:1436	n-butyric acid	1423:1436	n-butyric acid	1423:1436	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
34600701	7	39	theme	n-butyric	1423:1431	arg1	acid					1397:1400	acetic acid	1390:1400	acetic acid	1390:1400	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
34600701	3	40	theme	fermentation	433:444	arg1	properties					446:455	the digestion and fermentation properties	415:455	properties	446:455	In the present study, the digestion and fermentation properties of OPS and its effect on gut microbiota were evaluated using simulated digestion (saliva, stomach, and small intestine) and fermentation models in vitro.
34600701	1	41	theme	strong	274:279	arg1	activity					292:299	strong functional activity	274:299	strong functional activity	274:299	Oyster is a kind of nutritious shellfish widely consumed globally, and its polysaccharide (OPS) has been verified to have strong functional activity.
34600701	7	42	theme	acetic	1390:1395	arg1	metabolites					1452:1462	the main metabolites	1443:1462	the main metabolites	1443:1462	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
34600701	7	42	theme	acetic	1390:1395	arg1	acid					1413:1416	propionic acid	1403:1416	propionic acid	1403:1416	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
34600701	7	42	theme	acetic	1390:1395	arg1	acid					1433:1436	n-butyric acid	1423:1436	n-butyric acid	1423:1436	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
34600701	7	42	theme	acetic	1390:1395	arg1	acid					1397:1400	acetic acid	1390:1400	acetic acid	1390:1400	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
34600701	4	43	theme	sugar	729:733	arg1	content					735:741	the reducing sugar content	716:741	the reducing sugar content	716:741	The results showed that the molecular weight (Mw) of OPS decreased from 5.73 × 106 to 4.35 × 106 Da, and the reducing sugar content increased from 0.043 to 0.096 mg/mL, indicating that OPS was partially degraded during the saliva-gastrointestinal digestion.
34600701	6	44	dep	regulate	1099:1106	arg1	increase					1177:1184	increase	1177:1184	increase the abundance of beneficial bacteria such as Bacteroides, Prevotella, and Faecalibacterium	1177:1275	Notably, OPSI could regulate the composition and diversity of the microbial community, especially increase the abundance of beneficial bacteria such as Bacteroides, Prevotella, and Faecalibacterium.
34600701	1	45	theme	functional	281:290	arg1	activity					292:299	strong functional activity	274:299	strong functional activity	274:299	Oyster is a kind of nutritious shellfish widely consumed globally, and its polysaccharide (OPS) has been verified to have strong functional activity.
34600701	4	46	theme	molecular	639:647	arg1	Mw					657:658	Mw	657:658	Mw	657:658	The results showed that the molecular weight (Mw) of OPS decreased from 5.73 × 106 to 4.35 × 106 Da, and the reducing sugar content increased from 0.043 to 0.096 mg/mL, indicating that OPS was partially degraded during the saliva-gastrointestinal digestion.
34600701	4	46	theme	molecular	639:647	arg1	weight					649:654	the molecular weight	635:654	the molecular weight (Mw) of OPS	635:666	The results showed that the molecular weight (Mw) of OPS decreased from 5.73 × 106 to 4.35 × 106 Da, and the reducing sugar content increased from 0.043 to 0.096 mg/mL, indicating that OPS was partially degraded during the saliva-gastrointestinal digestion.
34600701	3	47	theme	digestion	419:427	arg1	properties					446:455	the digestion and fermentation properties	415:455	properties	446:455	In the present study, the digestion and fermentation properties of OPS and its effect on gut microbiota were evaluated using simulated digestion (saliva, stomach, and small intestine) and fermentation models in vitro.
34600701	4	48	theme	OPS	664:666	arg1	Mw					657:658	Mw	657:658	Mw	657:658	The results showed that the molecular weight (Mw) of OPS decreased from 5.73 × 106 to 4.35 × 106 Da, and the reducing sugar content increased from 0.043 to 0.096 mg/mL, indicating that OPS was partially degraded during the saliva-gastrointestinal digestion.
34600701	4	48	theme	OPS	664:666	arg1	weight					649:654	the molecular weight	635:654	the molecular weight (Mw) of OPS	635:666	The results showed that the molecular weight (Mw) of OPS decreased from 5.73 × 106 to 4.35 × 106 Da, and the reducing sugar content increased from 0.043 to 0.096 mg/mL, indicating that OPS was partially degraded during the saliva-gastrointestinal digestion.
34600701	6	49	theme	bacteria	1214:1221	arg1	abundance					1190:1198	the abundance	1186:1198	the abundance of beneficial bacteria such as Bacteroides, Prevotella, and Faecalibacterium	1186:1275	Notably, OPSI could regulate the composition and diversity of the microbial community, especially increase the abundance of beneficial bacteria such as Bacteroides, Prevotella, and Faecalibacterium.
34600701	5	50	theme	free	964:967	arg1	monosaccharides					969:983	free monosaccharides	964:983	free monosaccharides	964:983	During fermentation, the Mw and carbohydrate residue of indigestible OPS (OPSI) decreased, and free monosaccharides were released, suggesting that OPSI could be further degraded and utilized by gut microbiota.
34600701	3	51	theme	small	560:564	arg1	intestine					566:574	small intestine	560:574	small intestine	560:574	In the present study, the digestion and fermentation properties of OPS and its effect on gut microbiota were evaluated using simulated digestion (saliva, stomach, and small intestine) and fermentation models in vitro.
34600701	6	52	dep	composition	1112:1122	arg1	the					1108:1110	the	1108:1110	the	1108:1110	Notably, OPSI could regulate the composition and diversity of the microbial community, especially increase the abundance of beneficial bacteria such as Bacteroides, Prevotella, and Faecalibacterium.
34600701	0	53	from	effects	121:127	arg1	oyster					85:90	oyster	85:90	oyster (Crassostrea gigas)	85:110	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	0	53	from	effects	121:127	arg1	gigas					105:109	Crassostrea gigas	93:109	Crassostrea gigas	93:109	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	0	53	from	effects	121:127	arg1	microbiota					140:149	the gut microbiota	132:149	the gut microbiota	132:149	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	3	54	theme	in	601:602	arg1	models					594:599	fermentation models	581:599	fermentation models in vitro	581:608	In the present study, the digestion and fermentation properties of OPS and its effect on gut microbiota were evaluated using simulated digestion (saliva, stomach, and small intestine) and fermentation models in vitro.
34600701	2	55	dep	digestion	350:358	arg1	the					337:339	the	337:339	the	337:339	However, it is still unclear about the specific digestion and prebiotic properties of OPS.
34600701	3	56	dep	digestion	528:536	arg1	saliva					539:544	saliva	539:544	saliva	539:544	In the present study, the digestion and fermentation properties of OPS and its effect on gut microbiota were evaluated using simulated digestion (saliva, stomach, and small intestine) and fermentation models in vitro.
34600701	3	56	dep	digestion	528:536	arg1	intestine					566:574	small intestine	560:574	small intestine	560:574	In the present study, the digestion and fermentation properties of OPS and its effect on gut microbiota were evaluated using simulated digestion (saliva, stomach, and small intestine) and fermentation models in vitro.
34600701	3	56	dep	digestion	528:536	arg1	stomach					547:553	stomach	547:553	stomach	547:553	In the present study, the digestion and fermentation properties of OPS and its effect on gut microbiota were evaluated using simulated digestion (saliva, stomach, and small intestine) and fermentation models in vitro.
34600701	8	57	theme	digestive	1508:1516	arg1	characteristics					1518:1532	the digestive characteristics	1504:1532	the digestive characteristics of OPS	1504:1539	These results provided a reference for the digestive characteristics of OPS and revealed that OPS might be a potential prebiotic to prevent diseases by improving intestinal health.
34600701	0	58	theme	polysaccharide	65:78	arg1	effects					121:127	its effects	117:127	its effects on the gut microbiota	117:149	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	0	58	theme	polysaccharide	65:78	arg1	digestion					19:27	In vitro simulated digestion	0:27	In vitro simulated digestion	0:27	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	0	58	theme	polysaccharide	65:78	arg1	characteristics					46:60	fermentation characteristics	33:60	fermentation characteristics	33:60	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	0	59	from	digestion	19:27	arg1	oyster					85:90	oyster	85:90	oyster (Crassostrea gigas)	85:110	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	0	59	from	digestion	19:27	arg1	gigas					105:109	Crassostrea gigas	93:109	Crassostrea gigas	93:109	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	0	59	from	digestion	19:27	arg1	microbiota					140:149	the gut microbiota	132:149	the gut microbiota	132:149	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	0	60	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro simulated digestion and fermentation characteristics of polysaccharide from oyster (Crassostrea gigas), and its effects on the gut microbiota.
34600701	3	61	from	effect	472:477	arg1	microbiota					486:495	gut microbiota	482:495	gut microbiota	482:495	In the present study, the digestion and fermentation properties of OPS and its effect on gut microbiota were evaluated using simulated digestion (saliva, stomach, and small intestine) and fermentation models in vitro.
34600701	7	62	theme	propionic	1403:1411	arg1	acid					1413:1416	propionic acid	1403:1416	propionic acid	1403:1416	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
34600701	7	62	theme	propionic	1403:1411	arg1	metabolites					1452:1462	the main metabolites	1443:1462	the main metabolites	1443:1462	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
34600701	7	62	theme	propionic	1403:1411	arg1	acid					1433:1436	n-butyric acid	1423:1436	n-butyric acid	1423:1436	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
34600701	7	62	theme	propionic	1403:1411	arg1	acid					1397:1400	acetic acid	1390:1400	acetic acid	1390:1400	Additionally, after fermentation for 24 h, OPSI promoted the production of short-chain fatty acids (SCFAs), and acetic acid, propionic acid, and n-butyric acid were the main metabolites.
32885955	3	0	dep	approaches	352:361	arg1	analyzing					366:374	analyzing	366:374	to analyzing the structure of lignocellulose	363:406	Many current approaches to analyzing the structure of lignocellulose, which involve sequential extraction and characterization of the resulting fractions, are time-consuming and labor-intensive.
32885955	7	1	theme	procedure	1024:1032	arg1	advantage					1007:1015	A distinct advantage	996:1015	A distinct advantage of the procedure	996:1032	A distinct advantage of the procedure is that it realizes the dissolution of whole lignocellulosic materials without requiring harsh ball milling, thereby allowing the acquisition of high-resolution 2D NMR spectra to revealing structural details of the main components (lignin and polysaccharides).
32885955	6	2	from	DMSO-d6	867:873	arg1	products					842:849	The acetylated products	827:849	The acetylated products	827:849	The acetylated products were soluble in DMSO-d6 from which they can be characterized by solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry.
32885955	6	2	from	DMSO-d6	867:873	arg1	soluble					856:862	soluble	856:862	soluble	856:862	The acetylated products were soluble in DMSO-d6 from which they can be characterized by solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry.
32885955	7	3	theme	ball	1129:1132	arg1	milling					1134:1140	harsh ball milling	1123:1140	harsh ball milling	1123:1140	A distinct advantage of the procedure is that it realizes the dissolution of whole lignocellulosic materials without requiring harsh ball milling, thereby allowing the acquisition of high-resolution 2D NMR spectra to revealing structural details of the main components (lignin and polysaccharides).
32885955	0	4	from	Acetylation	5:15	arg1	EmimAc					72:77	EmimAc	72:77	EmimAc	72:77	Mild Acetylation and Solubilization of Ground Whole Plant Cell Walls in EmimAc: A Method for Solution-State NMR in DMSO-d6.
32885955	7	5	theme	high-resolution	1179:1193	arg1	spectra					1202:1208	high-resolution 2D NMR spectra	1179:1208	high-resolution 2D NMR spectra	1179:1208	A distinct advantage of the procedure is that it realizes the dissolution of whole lignocellulosic materials without requiring harsh ball milling, thereby allowing the acquisition of high-resolution 2D NMR spectra to revealing structural details of the main components (lignin and polysaccharides).
32885955	7	6	theme	harsh	1123:1127	arg1	milling					1134:1140	harsh ball milling	1123:1140	harsh ball milling	1123:1140	A distinct advantage of the procedure is that it realizes the dissolution of whole lignocellulosic materials without requiring harsh ball milling, thereby allowing the acquisition of high-resolution 2D NMR spectra to revealing structural details of the main components (lignin and polysaccharides).
32885955	2	7	from	components	293:302	arg1	form					312:315	pure form	307:315	pure form for characterization	307:336	The complexity and diversity of the plant cell wall polymers makes it difficult to isolate the components in pure form for characterization.
32885955	3	8	theme	Many	339:342	arg1	approaches					352:361	Many current approaches	339:361	Many current approaches	339:361	Many current approaches to analyzing the structure of lignocellulose, which involve sequential extraction and characterization of the resulting fractions, are time-consuming and labor-intensive.
32885955	6	9	theme	magnetic	954:961	arg1	resonance					963:971	two-dimensional nuclear magnetic resonance	930:971	solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry	915:993	The acetylated products were soluble in DMSO-d6 from which they can be characterized by solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry.
32885955	6	9	theme	magnetic	954:961	arg1	NMR					977:979	2D NMR	974:979	2D NMR	974:979	The acetylated products were soluble in DMSO-d6 from which they can be characterized by solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry.
32885955	5	10	theme	ionic	678:682	arg1	chloride					720:727	dichloroacetyl chloride	705:727	dichloroacetyl chloride	705:727	Using ionic liquids (EmimAc) and dichloroacetyl chloride as a solvent/reagent produced mildly acetylated whole cell walls without significant degradation.
32885955	5	10	theme	ionic	678:682	arg1	solvent/reagent					734:748	a solvent/reagent	732:748	a solvent/reagent	732:748	Using ionic liquids (EmimAc) and dichloroacetyl chloride as a solvent/reagent produced mildly acetylated whole cell walls without significant degradation.
32885955	5	10	theme	ionic	678:682	arg1	EmimAc					693:698	EmimAc	693:698	EmimAc	693:698	Using ionic liquids (EmimAc) and dichloroacetyl chloride as a solvent/reagent produced mildly acetylated whole cell walls without significant degradation.
32885955	5	10	theme	ionic	678:682	arg1	liquids					684:690	ionic liquids	678:690	ionic liquids (EmimAc)	678:699	Using ionic liquids (EmimAc) and dichloroacetyl chloride as a solvent/reagent produced mildly acetylated whole cell walls without significant degradation.
32885955	8	11	theme	improved	1401:1408	arg1	utilization					1410:1420	its improved utilization	1397:1420	its improved utilization	1397:1420	The method is therefore beneficial to understanding the composition and structure of biomass aimed at its improved utilization.
32885955	4	12	theme	derivatizing	601:612	arg1	materials					661:669	rationally derivatizing and dissolving coarsely ground plant cell wall materials	590:669	rationally derivatizing and dissolving coarsely ground plant cell wall materials	590:669	The present study describes a new and facile system for rationally derivatizing and dissolving coarsely ground plant cell wall materials.
32885955	3	13	theme	current	344:350	arg1	approaches					352:361	Many current approaches	339:361	Many current approaches	339:361	Many current approaches to analyzing the structure of lignocellulose, which involve sequential extraction and characterization of the resulting fractions, are time-consuming and labor-intensive.
32885955	6	14	theme	nuclear	946:952	arg1	resonance					963:971	two-dimensional nuclear magnetic resonance	930:971	solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry	915:993	The acetylated products were soluble in DMSO-d6 from which they can be characterized by solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry.
32885955	6	14	theme	nuclear	946:952	arg1	NMR					977:979	2D NMR	974:979	2D NMR	974:979	The acetylated products were soluble in DMSO-d6 from which they can be characterized by solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry.
32885955	7	15	theme	spectra	1202:1208	arg1	acquisition					1164:1174	the acquisition	1160:1174	the acquisition of high-resolution 2D NMR spectra to revealing structural details of the main components (lignin and polysaccharides)	1160:1292	A distinct advantage of the procedure is that it realizes the dissolution of whole lignocellulosic materials without requiring harsh ball milling, thereby allowing the acquisition of high-resolution 2D NMR spectra to revealing structural details of the main components (lignin and polysaccharides).
32885955	8	16	dep	composition	1351:1361	arg1	the					1347:1349	the	1347:1349	the	1347:1349	The method is therefore beneficial to understanding the composition and structure of biomass aimed at its improved utilization.
32885955	6	17	theme	two-dimensional	930:944	arg1	resonance					963:971	two-dimensional nuclear magnetic resonance	930:971	solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry	915:993	The acetylated products were soluble in DMSO-d6 from which they can be characterized by solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry.
32885955	6	17	theme	two-dimensional	930:944	arg1	NMR					977:979	2D NMR	974:979	2D NMR	974:979	The acetylated products were soluble in DMSO-d6 from which they can be characterized by solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry.
32885955	6	18	theme	acetylated	831:840	arg1	products					842:849	The acetylated products	827:849	The acetylated products	827:849	The acetylated products were soluble in DMSO-d6 from which they can be characterized by solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry.
32885955	6	18	theme	acetylated	831:840	arg1	soluble					856:862	soluble	856:862	soluble	856:862	The acetylated products were soluble in DMSO-d6 from which they can be characterized by solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry.
32885955	3	19	theme	sequential	423:432	arg1	extraction					434:443	sequential extraction	423:443	sequential extraction	423:443	Many current approaches to analyzing the structure of lignocellulose, which involve sequential extraction and characterization of the resulting fractions, are time-consuming and labor-intensive.
32885955	5	20	theme	acetylated	766:775	arg1	walls					788:792	mildly acetylated whole cell walls	759:792	mildly acetylated whole cell walls	759:792	Using ionic liquids (EmimAc) and dichloroacetyl chloride as a solvent/reagent produced mildly acetylated whole cell walls without significant degradation.
32885955	6	21	theme	solution-state	915:928	arg1	spectrometry					982:993	solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry	915:993	solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry	915:993	The acetylated products were soluble in DMSO-d6 from which they can be characterized by solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry.
32885955	0	22	theme	Ground	39:44	arg1	Walls					63:67	Ground Whole Plant Cell Walls	39:67	Ground Whole Plant Cell Walls	39:67	Mild Acetylation and Solubilization of Ground Whole Plant Cell Walls in EmimAc: A Method for Solution-State NMR in DMSO-d6.
32885955	2	23	theme	polymers	250:257	arg1	diversity					217:225	diversity	217:225	diversity	217:225	The complexity and diversity of the plant cell wall polymers makes it difficult to isolate the components in pure form for characterization.
32885955	2	23	theme	polymers	250:257	arg1	complexity					202:211	The complexity	198:211	The complexity	198:211	The complexity and diversity of the plant cell wall polymers makes it difficult to isolate the components in pure form for characterization.
32885955	5	24	theme	whole	777:781	arg1	walls					788:792	mildly acetylated whole cell walls	759:792	mildly acetylated whole cell walls	759:792	Using ionic liquids (EmimAc) and dichloroacetyl chloride as a solvent/reagent produced mildly acetylated whole cell walls without significant degradation.
32885955	6	25	from	soluble	856:862	arg1	DMSO-d6					867:873	DMSO-d6	867:873	DMSO-d6 from which they can be characterized by solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry	867:993	The acetylated products were soluble in DMSO-d6 from which they can be characterized by solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry.
32885955	0	26	theme	Mild	0:3	arg1	Acetylation					5:15	Mild Acetylation	0:15	Mild Acetylation	0:15	Mild Acetylation and Solubilization of Ground Whole Plant Cell Walls in EmimAc: A Method for Solution-State NMR in DMSO-d6.
32885955	4	27	theme	new	564:566	arg1	system					579:584	a new and facile system	562:584	a new and facile system for rationally derivatizing and dissolving coarsely ground plant cell wall materials	562:669	The present study describes a new and facile system for rationally derivatizing and dissolving coarsely ground plant cell wall materials.
32885955	0	28	dep	Acetylation	5:15	arg1	Method					82:87	A Method	80:87	Mild Acetylation and Solubilization of Ground Whole Plant Cell Walls in EmimAc: A Method for Solution-State NMR in DMSO-d6.	0:122	Mild Acetylation and Solubilization of Ground Whole Plant Cell Walls in EmimAc: A Method for Solution-State NMR in DMSO-d6.
32885955	5	29	theme	dichloroacetyl	705:718	arg1	chloride					720:727	dichloroacetyl chloride	705:727	dichloroacetyl chloride	705:727	Using ionic liquids (EmimAc) and dichloroacetyl chloride as a solvent/reagent produced mildly acetylated whole cell walls without significant degradation.
32885955	5	29	theme	dichloroacetyl	705:718	arg1	solvent/reagent					734:748	a solvent/reagent	732:748	a solvent/reagent	732:748	Using ionic liquids (EmimAc) and dichloroacetyl chloride as a solvent/reagent produced mildly acetylated whole cell walls without significant degradation.
32885955	5	29	theme	dichloroacetyl	705:718	arg1	liquids					684:690	ionic liquids	678:690	ionic liquids (EmimAc)	678:699	Using ionic liquids (EmimAc) and dichloroacetyl chloride as a solvent/reagent produced mildly acetylated whole cell walls without significant degradation.
32885955	7	30	theme	distinct	998:1005	arg1	advantage					1007:1015	A distinct advantage	996:1015	A distinct advantage of the procedure	996:1032	A distinct advantage of the procedure is that it realizes the dissolution of whole lignocellulosic materials without requiring harsh ball milling, thereby allowing the acquisition of high-resolution 2D NMR spectra to revealing structural details of the main components (lignin and polysaccharides).
32885955	4	31	theme	cell	651:654	arg1	materials					661:669	rationally derivatizing and dissolving coarsely ground plant cell wall materials	590:669	rationally derivatizing and dissolving coarsely ground plant cell wall materials	590:669	The present study describes a new and facile system for rationally derivatizing and dissolving coarsely ground plant cell wall materials.
32885955	7	32	theme	structural	1223:1232	arg1	details					1234:1240	structural details	1223:1240	structural details of the main components (lignin and polysaccharides)	1223:1292	A distinct advantage of the procedure is that it realizes the dissolution of whole lignocellulosic materials without requiring harsh ball milling, thereby allowing the acquisition of high-resolution 2D NMR spectra to revealing structural details of the main components (lignin and polysaccharides).
32885955	4	33	theme	facile	572:577	arg1	system					579:584	a new and facile system	562:584	a new and facile system for rationally derivatizing and dissolving coarsely ground plant cell wall materials	562:669	The present study describes a new and facile system for rationally derivatizing and dissolving coarsely ground plant cell wall materials.
32885955	0	34	theme	Solution-State	93:106	arg1	NMR					108:110	Solution-State NMR	93:110	Solution-State NMR in DMSO-d6	93:121	Mild Acetylation and Solubilization of Ground Whole Plant Cell Walls in EmimAc: A Method for Solution-State NMR in DMSO-d6.
32885955	7	35	theme	whole	1073:1077	arg1	materials					1095:1103	whole lignocellulosic materials	1073:1103	whole lignocellulosic materials	1073:1103	A distinct advantage of the procedure is that it realizes the dissolution of whole lignocellulosic materials without requiring harsh ball milling, thereby allowing the acquisition of high-resolution 2D NMR spectra to revealing structural details of the main components (lignin and polysaccharides).
32885955	4	36	theme	plant	645:649	arg1	materials					661:669	rationally derivatizing and dissolving coarsely ground plant cell wall materials	590:669	rationally derivatizing and dissolving coarsely ground plant cell wall materials	590:669	The present study describes a new and facile system for rationally derivatizing and dissolving coarsely ground plant cell wall materials.
32885955	3	37	theme	lignocellulose	393:406	arg1	structure					380:388	the structure	376:388	the structure of lignocellulose	376:406	Many current approaches to analyzing the structure of lignocellulose, which involve sequential extraction and characterization of the resulting fractions, are time-consuming and labor-intensive.
32885955	7	38	theme	main	1249:1252	arg1	components					1254:1263	the main components	1245:1263	the main components (lignin and polysaccharides)	1245:1292	A distinct advantage of the procedure is that it realizes the dissolution of whole lignocellulosic materials without requiring harsh ball milling, thereby allowing the acquisition of high-resolution 2D NMR spectra to revealing structural details of the main components (lignin and polysaccharides).
32885955	7	38	theme	main	1249:1252	arg1	polysaccharides					1277:1291	polysaccharides	1277:1291	polysaccharides	1277:1291	A distinct advantage of the procedure is that it realizes the dissolution of whole lignocellulosic materials without requiring harsh ball milling, thereby allowing the acquisition of high-resolution 2D NMR spectra to revealing structural details of the main components (lignin and polysaccharides).
32885955	7	38	theme	main	1249:1252	arg1	lignin					1266:1271	lignin	1266:1271	lignin	1266:1271	A distinct advantage of the procedure is that it realizes the dissolution of whole lignocellulosic materials without requiring harsh ball milling, thereby allowing the acquisition of high-resolution 2D NMR spectra to revealing structural details of the main components (lignin and polysaccharides).
32885955	7	39	theme	2D	1195:1196	arg1	spectra					1202:1208	high-resolution 2D NMR spectra	1179:1208	high-resolution 2D NMR spectra	1179:1208	A distinct advantage of the procedure is that it realizes the dissolution of whole lignocellulosic materials without requiring harsh ball milling, thereby allowing the acquisition of high-resolution 2D NMR spectra to revealing structural details of the main components (lignin and polysaccharides).
32885955	4	40	theme	present	538:544	arg1	study					546:550	The present study	534:550	The present study	534:550	The present study describes a new and facile system for rationally derivatizing and dissolving coarsely ground plant cell wall materials.
32885955	4	41	theme	ground	638:643	arg1	materials					661:669	rationally derivatizing and dissolving coarsely ground plant cell wall materials	590:669	rationally derivatizing and dissolving coarsely ground plant cell wall materials	590:669	The present study describes a new and facile system for rationally derivatizing and dissolving coarsely ground plant cell wall materials.
32885955	4	42	theme	wall	656:659	arg1	materials					661:669	rationally derivatizing and dissolving coarsely ground plant cell wall materials	590:669	rationally derivatizing and dissolving coarsely ground plant cell wall materials	590:669	The present study describes a new and facile system for rationally derivatizing and dissolving coarsely ground plant cell wall materials.
32885955	0	43	theme	Plant	52:56	arg1	Walls					63:67	Ground Whole Plant Cell Walls	39:67	Ground Whole Plant Cell Walls	39:67	Mild Acetylation and Solubilization of Ground Whole Plant Cell Walls in EmimAc: A Method for Solution-State NMR in DMSO-d6.
32885955	7	44	theme	components	1254:1263	arg1	details					1234:1240	structural details	1223:1240	structural details of the main components (lignin and polysaccharides)	1223:1292	A distinct advantage of the procedure is that it realizes the dissolution of whole lignocellulosic materials without requiring harsh ball milling, thereby allowing the acquisition of high-resolution 2D NMR spectra to revealing structural details of the main components (lignin and polysaccharides).
32885955	7	45	theme	lignocellulosic	1079:1093	arg1	materials					1095:1103	whole lignocellulosic materials	1073:1103	whole lignocellulosic materials	1073:1103	A distinct advantage of the procedure is that it realizes the dissolution of whole lignocellulosic materials without requiring harsh ball milling, thereby allowing the acquisition of high-resolution 2D NMR spectra to revealing structural details of the main components (lignin and polysaccharides).
32885955	5	46	theme	cell	783:786	arg1	walls					788:792	mildly acetylated whole cell walls	759:792	mildly acetylated whole cell walls	759:792	Using ionic liquids (EmimAc) and dichloroacetyl chloride as a solvent/reagent produced mildly acetylated whole cell walls without significant degradation.
32885955	7	47	dep	components	1254:1263	arg1	components					1254:1263	the main components	1245:1263	the main components (lignin and polysaccharides)	1245:1292	A distinct advantage of the procedure is that it realizes the dissolution of whole lignocellulosic materials without requiring harsh ball milling, thereby allowing the acquisition of high-resolution 2D NMR spectra to revealing structural details of the main components (lignin and polysaccharides).
32885955	7	47	dep	components	1254:1263	arg1	polysaccharides					1277:1291	polysaccharides	1277:1291	polysaccharides	1277:1291	A distinct advantage of the procedure is that it realizes the dissolution of whole lignocellulosic materials without requiring harsh ball milling, thereby allowing the acquisition of high-resolution 2D NMR spectra to revealing structural details of the main components (lignin and polysaccharides).
32885955	7	47	dep	components	1254:1263	arg1	lignin					1266:1271	lignin	1266:1271	lignin	1266:1271	A distinct advantage of the procedure is that it realizes the dissolution of whole lignocellulosic materials without requiring harsh ball milling, thereby allowing the acquisition of high-resolution 2D NMR spectra to revealing structural details of the main components (lignin and polysaccharides).
32885955	0	48	theme	Whole	46:50	arg1	Walls					63:67	Ground Whole Plant Cell Walls	39:67	Ground Whole Plant Cell Walls	39:67	Mild Acetylation and Solubilization of Ground Whole Plant Cell Walls in EmimAc: A Method for Solution-State NMR in DMSO-d6.
32885955	7	49	theme	materials	1095:1103	arg1	dissolution					1058:1068	the dissolution	1054:1068	the dissolution of whole lignocellulosic materials	1054:1103	A distinct advantage of the procedure is that it realizes the dissolution of whole lignocellulosic materials without requiring harsh ball milling, thereby allowing the acquisition of high-resolution 2D NMR spectra to revealing structural details of the main components (lignin and polysaccharides).
32885955	6	50	theme	resonance	963:971	arg1	spectrometry					982:993	solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry	915:993	solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry	915:993	The acetylated products were soluble in DMSO-d6 from which they can be characterized by solution-state two-dimensional nuclear magnetic resonance (2D NMR) spectrometry.
32885955	7	51	theme	NMR	1198:1200	arg1	spectra					1202:1208	high-resolution 2D NMR spectra	1179:1208	high-resolution 2D NMR spectra	1179:1208	A distinct advantage of the procedure is that it realizes the dissolution of whole lignocellulosic materials without requiring harsh ball milling, thereby allowing the acquisition of high-resolution 2D NMR spectra to revealing structural details of the main components (lignin and polysaccharides).
32885955	2	52	theme	wall	245:248	arg1	polymers					250:257	the plant cell wall polymers	230:257	the plant cell wall polymers	230:257	The complexity and diversity of the plant cell wall polymers makes it difficult to isolate the components in pure form for characterization.
32885955	2	53	theme	pure	307:310	arg1	form					312:315	pure form	307:315	pure form for characterization	307:336	The complexity and diversity of the plant cell wall polymers makes it difficult to isolate the components in pure form for characterization.
32885955	5	54	theme	significant	802:812	arg1	degradation					814:824	significant degradation	802:824	significant degradation	802:824	Using ionic liquids (EmimAc) and dichloroacetyl chloride as a solvent/reagent produced mildly acetylated whole cell walls without significant degradation.
32885955	2	55	theme	cell	240:243	arg1	polymers					250:257	the plant cell wall polymers	230:257	the plant cell wall polymers	230:257	The complexity and diversity of the plant cell wall polymers makes it difficult to isolate the components in pure form for characterization.
32885955	3	56	theme	fractions	483:491	arg1	characterization					449:464	characterization	449:464	characterization	449:464	Many current approaches to analyzing the structure of lignocellulose, which involve sequential extraction and characterization of the resulting fractions, are time-consuming and labor-intensive.
32885955	3	56	theme	fractions	483:491	arg1	extraction					434:443	sequential extraction	423:443	sequential extraction	423:443	Many current approaches to analyzing the structure of lignocellulose, which involve sequential extraction and characterization of the resulting fractions, are time-consuming and labor-intensive.
32885955	3	57	theme	resulting	473:481	arg1	fractions					483:491	the resulting fractions	469:491	the resulting fractions	469:491	Many current approaches to analyzing the structure of lignocellulose, which involve sequential extraction and characterization of the resulting fractions, are time-consuming and labor-intensive.
32885955	0	58	theme	Walls	63:67	arg1	Solubilization					21:34	Solubilization	21:34	Solubilization	21:34	Mild Acetylation and Solubilization of Ground Whole Plant Cell Walls in EmimAc: A Method for Solution-State NMR in DMSO-d6.
32885955	0	58	theme	Walls	63:67	arg1	Acetylation					5:15	Mild Acetylation	0:15	Mild Acetylation	0:15	Mild Acetylation and Solubilization of Ground Whole Plant Cell Walls in EmimAc: A Method for Solution-State NMR in DMSO-d6.
32885955	2	59	theme	plant	234:238	arg1	polymers					250:257	the plant cell wall polymers	230:257	the plant cell wall polymers	230:257	The complexity and diversity of the plant cell wall polymers makes it difficult to isolate the components in pure form for characterization.
32885955	8	60	theme	biomass	1380:1386	arg1	structure					1367:1375	structure	1367:1375	structure	1367:1375	The method is therefore beneficial to understanding the composition and structure of biomass aimed at its improved utilization.
32885955	8	60	theme	biomass	1380:1386	arg1	composition					1351:1361	composition	1351:1361	composition	1351:1361	The method is therefore beneficial to understanding the composition and structure of biomass aimed at its improved utilization.
32885955	0	61	from	NMR	108:110	arg1	DMSO-d6					115:121	DMSO-d6	115:121	DMSO-d6	115:121	Mild Acetylation and Solubilization of Ground Whole Plant Cell Walls in EmimAc: A Method for Solution-State NMR in DMSO-d6.
32885955	0	62	theme	Cell	58:61	arg1	Walls					63:67	Ground Whole Plant Cell Walls	39:67	Ground Whole Plant Cell Walls	39:67	Mild Acetylation and Solubilization of Ground Whole Plant Cell Walls in EmimAc: A Method for Solution-State NMR in DMSO-d6.
32885955	0	63	from	Solubilization	21:34	arg1	EmimAc					72:77	EmimAc	72:77	EmimAc	72:77	Mild Acetylation and Solubilization of Ground Whole Plant Cell Walls in EmimAc: A Method for Solution-State NMR in DMSO-d6.
32885955	1	64	theme	Lignocellulosic	124:138	arg1	biomass					140:146	Lignocellulosic biomass	124:146	Lignocellulosic biomass	124:146	Lignocellulosic biomass is mainly composed of polysaccharides and lignin.
32885955	4	65	theme	coarsely	629:636	arg1	materials					661:669	rationally derivatizing and dissolving coarsely ground plant cell wall materials	590:669	rationally derivatizing and dissolving coarsely ground plant cell wall materials	590:669	The present study describes a new and facile system for rationally derivatizing and dissolving coarsely ground plant cell wall materials.
32168852	0	0	theme	Fermented	78:86	arg1	Mycelia					88:94	Fermented Mycelia	78:94	Fermented Mycelia	78:94	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos.
32168852	4	1	from	WPS	556:558	arg1	mycelia					635:641	fermented mycelia	625:641	fermented mycelia	625:641	In this study, WPS, triterpenoids, monosaccharide composition, and essential oil in fermented mycelia and cultivated sclerotium were analyzed using UV spectrophotometry, HPLC, pre-column derivatization, and HS-GC/MS, respectively.
32168852	4	1	from	WPS	556:558	arg1	sclerotium					658:667	cultivated sclerotium	647:667	cultivated sclerotium	647:667	In this study, WPS, triterpenoids, monosaccharide composition, and essential oil in fermented mycelia and cultivated sclerotium were analyzed using UV spectrophotometry, HPLC, pre-column derivatization, and HS-GC/MS, respectively.
32168852	6	2	from	fragrances	1040:1049	arg1	common					1054:1059	common	1054:1059	common	1054:1059	Among the 62 compounds identified by HS-GC/MS analysis from the essential oil obtained from the fermentation media and a fresh external layer, the two main fragrances in common were linalool and methyl phenylacetate.
32168852	4	3	theme	cultivated	647:656	arg1	sclerotium					658:667	cultivated sclerotium	647:667	cultivated sclerotium	647:667	In this study, WPS, triterpenoids, monosaccharide composition, and essential oil in fermented mycelia and cultivated sclerotium were analyzed using UV spectrophotometry, HPLC, pre-column derivatization, and HS-GC/MS, respectively.
32168852	6	4	theme	fresh	1005:1009	arg1	layer					1020:1024	a fresh external layer	1003:1024	a fresh external layer	1003:1024	Among the 62 compounds identified by HS-GC/MS analysis from the essential oil obtained from the fermentation media and a fresh external layer, the two main fragrances in common were linalool and methyl phenylacetate.
32168852	0	5	from	Studies	12:18	arg1	Triterpenoids					40:52	Triterpenoids	40:52	Triterpenoids	40:52	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos.
32168852	0	5	from	Studies	12:18	arg1	Polysaccharides					23:37	Polysaccharides	23:37	Polysaccharides	23:37	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos.
32168852	0	5	from	Studies	12:18	arg1	Oil					69:71	Essential Oil	59:71	Essential Oil	59:71	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos.
32168852	5	6	theme	higher	851:856	arg1	WPS					800:802	WPS	800:802	WPS	800:802	Our results showed that the WPS and triterpenoids in mycelia are several times higher than those in sclerotium.
32168852	5	6	theme	higher	851:856	arg1	times					845:849	several times	837:849	several times higher than those in sclerotium	837:881	Our results showed that the WPS and triterpenoids in mycelia are several times higher than those in sclerotium.
32168852	5	6	theme	higher	851:856	arg1	triterpenoids					808:820	triterpenoids	808:820	triterpenoids	808:820	Our results showed that the WPS and triterpenoids in mycelia are several times higher than those in sclerotium.
32168852	6	7	theme	essential	948:956	arg1	oil					958:960	the essential oil	944:960	the essential oil obtained from the fermentation media	944:997	Among the 62 compounds identified by HS-GC/MS analysis from the essential oil obtained from the fermentation media and a fresh external layer, the two main fragrances in common were linalool and methyl phenylacetate.
32168852	6	8	theme	main	1035:1038	arg1	fragrances					1040:1049	the two main fragrances	1027:1049	the two main fragrances in common	1027:1059	Among the 62 compounds identified by HS-GC/MS analysis from the essential oil obtained from the fermentation media and a fresh external layer, the two main fragrances in common were linalool and methyl phenylacetate.
32168852	6	8	theme	main	1035:1038	arg1	linalool					1066:1073	linalool	1066:1073	linalool	1066:1073	Among the 62 compounds identified by HS-GC/MS analysis from the essential oil obtained from the fermentation media and a fresh external layer, the two main fragrances in common were linalool and methyl phenylacetate.
32168852	4	9	theme	pre-column	717:726	arg1	derivatization					728:741	pre-column derivatization	717:741	pre-column derivatization	717:741	In this study, WPS, triterpenoids, monosaccharide composition, and essential oil in fermented mycelia and cultivated sclerotium were analyzed using UV spectrophotometry, HPLC, pre-column derivatization, and HS-GC/MS, respectively.
32168852	6	10	theme	methyl	1079:1084	arg1	phenylacetate					1086:1098	methyl phenylacetate	1079:1098	methyl phenylacetate	1079:1098	Among the 62 compounds identified by HS-GC/MS analysis from the essential oil obtained from the fermentation media and a fresh external layer, the two main fragrances in common were linalool and methyl phenylacetate.
32168852	3	11	theme	active	499:504	arg1	components					506:515	its active components	495:515	its active components	495:515	Due to the growing market demand, long cultivation period, and consumption of pine trunk during cultivation, alternative methods for producing P. cocos or its active components should be investigated.
32168852	7	12	theme	triterpenoid	1249:1260	arg1	production					1262:1271	triterpenoid production	1249:1271	triterpenoid production in the fermentation process	1249:1299	Our results suggested that it is applicable to produce polysaccharides and triterpenoids by the fermentation of P. cocos, and a strategy to improve triterpenoid production in the fermentation process was proposed.
32168852	3	13	theme	cultivation	379:389	arg1	period					391:396	long cultivation period	374:396	long cultivation period	374:396	Due to the growing market demand, long cultivation period, and consumption of pine trunk during cultivation, alternative methods for producing P. cocos or its active components should be investigated.
32168852	4	14	theme	essential	608:616	arg1	oil					618:620	essential oil	608:620	essential oil	608:620	In this study, WPS, triterpenoids, monosaccharide composition, and essential oil in fermented mycelia and cultivated sclerotium were analyzed using UV spectrophotometry, HPLC, pre-column derivatization, and HS-GC/MS, respectively.
32168852	0	15	theme	Cultivated	100:109	arg1	Sclerotium					111:120	Cultivated Sclerotium	100:120	Cultivated Sclerotium	100:120	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos.
32168852	3	16	theme	growing	351:357	arg1	demand					366:371	the growing market demand	347:371	the growing market demand	347:371	Due to the growing market demand, long cultivation period, and consumption of pine trunk during cultivation, alternative methods for producing P. cocos or its active components should be investigated.
32168852	7	17	theme	fermentation	1280:1291	arg1	process					1293:1299	the fermentation process	1276:1299	the fermentation process	1276:1299	Our results suggested that it is applicable to produce polysaccharides and triterpenoids by the fermentation of P. cocos, and a strategy to improve triterpenoid production in the fermentation process was proposed.
32168852	1	18	theme	Poria	171:175	arg1	fungus					218:223	an important medicinal and edible fungus	184:223	an important medicinal and edible fungus	184:223	Poria cocos, an important medicinal and edible fungus, is well known in East Asia.
32168852	1	18	theme	Poria	171:175	arg1	cocos					177:181	Poria cocos	171:181	Poria cocos	171:181	Poria cocos, an important medicinal and edible fungus, is well known in East Asia.
32168852	0	19	theme	Comparative	0:10	arg1	Studies					12:18	Comparative Studies	0:18	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom	0:155	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos.
32168852	1	20	theme	East	243:246	arg1	Asia					248:251	East Asia	243:251	East Asia	243:251	Poria cocos, an important medicinal and edible fungus, is well known in East Asia.
32168852	0	21	from	Mycelia	88:94	arg1	Triterpenoids					40:52	Triterpenoids	40:52	Triterpenoids	40:52	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos.
32168852	0	21	from	Mycelia	88:94	arg1	Polysaccharides					23:37	Polysaccharides	23:37	Polysaccharides	23:37	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos.
32168852	0	21	from	Mycelia	88:94	arg1	Oil					69:71	Essential Oil	59:71	Essential Oil	59:71	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos.
32168852	4	22	theme	UV	689:690	arg1	spectrophotometry					692:708	UV spectrophotometry	689:708	UV spectrophotometry	689:708	In this study, WPS, triterpenoids, monosaccharide composition, and essential oil in fermented mycelia and cultivated sclerotium were analyzed using UV spectrophotometry, HPLC, pre-column derivatization, and HS-GC/MS, respectively.
32168852	6	23	theme	external	1011:1018	arg1	layer					1020:1024	a fresh external layer	1003:1024	a fresh external layer	1003:1024	Among the 62 compounds identified by HS-GC/MS analysis from the essential oil obtained from the fermentation media and a fresh external layer, the two main fragrances in common were linalool and methyl phenylacetate.
32168852	6	24	theme	HS-GC/MS	921:928	arg1	analysis					930:937	HS-GC/MS analysis	921:937	HS-GC/MS analysis	921:937	Among the 62 compounds identified by HS-GC/MS analysis from the essential oil obtained from the fermentation media and a fresh external layer, the two main fragrances in common were linalool and methyl phenylacetate.
32168852	2	25	theme	active	263:268	arg1	components					270:279	The main active components	254:279	The main active components	254:279	The main active components are water-soluble polysaccharides (WPS) and triterpenoids.
32168852	2	25	theme	active	263:268	arg1	polysaccharides					299:313	water-soluble polysaccharides	285:313	water-soluble polysaccharides (WPS)	285:319	The main active components are water-soluble polysaccharides (WPS) and triterpenoids.
32168852	4	26	from	composition	591:601	arg1	mycelia					635:641	fermented mycelia	625:641	fermented mycelia	625:641	In this study, WPS, triterpenoids, monosaccharide composition, and essential oil in fermented mycelia and cultivated sclerotium were analyzed using UV spectrophotometry, HPLC, pre-column derivatization, and HS-GC/MS, respectively.
32168852	4	26	from	composition	591:601	arg1	sclerotium					658:667	cultivated sclerotium	647:667	cultivated sclerotium	647:667	In this study, WPS, triterpenoids, monosaccharide composition, and essential oil in fermented mycelia and cultivated sclerotium were analyzed using UV spectrophotometry, HPLC, pre-column derivatization, and HS-GC/MS, respectively.
32168852	3	27	theme	pine	418:421	arg1	trunk					423:427	pine trunk	418:427	pine trunk	418:427	Due to the growing market demand, long cultivation period, and consumption of pine trunk during cultivation, alternative methods for producing P. cocos or its active components should be investigated.
32168852	2	28	theme	main	258:261	arg1	components					270:279	The main active components	254:279	The main active components	254:279	The main active components are water-soluble polysaccharides (WPS) and triterpenoids.
32168852	2	28	theme	main	258:261	arg1	polysaccharides					299:313	water-soluble polysaccharides	285:313	water-soluble polysaccharides (WPS)	285:319	The main active components are water-soluble polysaccharides (WPS) and triterpenoids.
32168852	3	29	theme	trunk	423:427	arg1	demand					366:371	the growing market demand	347:371	the growing market demand	347:371	Due to the growing market demand, long cultivation period, and consumption of pine trunk during cultivation, alternative methods for producing P. cocos or its active components should be investigated.
32168852	3	29	theme	trunk	423:427	arg1	period					391:396	long cultivation period	374:396	long cultivation period	374:396	Due to the growing market demand, long cultivation period, and consumption of pine trunk during cultivation, alternative methods for producing P. cocos or its active components should be investigated.
32168852	3	29	theme	trunk	423:427	arg1	consumption					403:413	consumption	403:413	consumption of pine trunk during cultivation	403:446	Due to the growing market demand, long cultivation period, and consumption of pine trunk during cultivation, alternative methods for producing P. cocos or its active components should be investigated.
32168852	4	30	from	oil	618:620	arg1	mycelia					635:641	fermented mycelia	625:641	fermented mycelia	625:641	In this study, WPS, triterpenoids, monosaccharide composition, and essential oil in fermented mycelia and cultivated sclerotium were analyzed using UV spectrophotometry, HPLC, pre-column derivatization, and HS-GC/MS, respectively.
32168852	4	30	from	oil	618:620	arg1	sclerotium					658:667	cultivated sclerotium	647:667	cultivated sclerotium	647:667	In this study, WPS, triterpenoids, monosaccharide composition, and essential oil in fermented mycelia and cultivated sclerotium were analyzed using UV spectrophotometry, HPLC, pre-column derivatization, and HS-GC/MS, respectively.
32168852	1	31	theme	important	187:195	arg1	fungus					218:223	an important medicinal and edible fungus	184:223	an important medicinal and edible fungus	184:223	Poria cocos, an important medicinal and edible fungus, is well known in East Asia.
32168852	1	31	theme	important	187:195	arg1	cocos					177:181	Poria cocos	171:181	Poria cocos	171:181	Poria cocos, an important medicinal and edible fungus, is well known in East Asia.
32168852	3	32	theme	long	374:377	arg1	period					391:396	long cultivation period	374:396	long cultivation period	374:396	Due to the growing market demand, long cultivation period, and consumption of pine trunk during cultivation, alternative methods for producing P. cocos or its active components should be investigated.
32168852	0	33	theme	Medicinal	127:135	arg1	Mushroom					148:155	a Medicinal and Edible Mushroom	125:155	Mushroom	148:155	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos.
32168852	5	34	from	WPS	800:802	arg1	mycelia					825:831	mycelia	825:831	mycelia	825:831	Our results showed that the WPS and triterpenoids in mycelia are several times higher than those in sclerotium.
32168852	0	35	dep	Cocos	164:168	arg1	Studies					12:18	Comparative Studies	0:18	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom	0:155	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos.
32168852	3	36	theme	market	359:364	arg1	demand					366:371	the growing market demand	347:371	the growing market demand	347:371	Due to the growing market demand, long cultivation period, and consumption of pine trunk during cultivation, alternative methods for producing P. cocos or its active components should be investigated.
32168852	0	37	theme	Mushroom	148:155	arg1	Mycelia					88:94	Fermented Mycelia	78:94	Fermented Mycelia	78:94	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos.
32168852	0	37	theme	Mushroom	148:155	arg1	Sclerotium					111:120	Cultivated Sclerotium	100:120	Cultivated Sclerotium	100:120	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos.
32168852	0	38	theme	Edible	141:146	arg1	Mushroom					148:155	a Medicinal and Edible Mushroom	125:155	Mushroom	148:155	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos.
32168852	0	39	from	Sclerotium	111:120	arg1	Triterpenoids					40:52	Triterpenoids	40:52	Triterpenoids	40:52	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos.
32168852	0	39	from	Sclerotium	111:120	arg1	Polysaccharides					23:37	Polysaccharides	23:37	Polysaccharides	23:37	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos.
32168852	0	39	from	Sclerotium	111:120	arg1	Oil					69:71	Essential Oil	59:71	Essential Oil	59:71	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos.
32168852	3	40	theme	alternative	449:459	arg1	methods					461:467	alternative methods	449:467	alternative methods for producing P. cocos or its active components	449:515	Due to the growing market demand, long cultivation period, and consumption of pine trunk during cultivation, alternative methods for producing P. cocos or its active components should be investigated.
32168852	7	41	theme	P.	1213:1214	arg1	cocos					1216:1220	P. cocos	1213:1220	P. cocos	1213:1220	Our results suggested that it is applicable to produce polysaccharides and triterpenoids by the fermentation of P. cocos, and a strategy to improve triterpenoid production in the fermentation process was proposed.
32168852	0	42	theme	Poria	158:162	arg1	Cocos					164:168	Poria Cocos	158:168	Poria Cocos	158:168	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos.
32168852	7	43	dep	strategy	1229:1236	arg1	improve					1241:1247	improve	1241:1247	to improve triterpenoid production in the fermentation process	1238:1299	Our results suggested that it is applicable to produce polysaccharides and triterpenoids by the fermentation of P. cocos, and a strategy to improve triterpenoid production in the fermentation process was proposed.
32168852	5	44	dep	WPS	800:802	arg1	the					796:798	the	796:798	the	796:798	Our results showed that the WPS and triterpenoids in mycelia are several times higher than those in sclerotium.
32168852	4	45	theme	fermented	625:633	arg1	mycelia					635:641	fermented mycelia	625:641	fermented mycelia	625:641	In this study, WPS, triterpenoids, monosaccharide composition, and essential oil in fermented mycelia and cultivated sclerotium were analyzed using UV spectrophotometry, HPLC, pre-column derivatization, and HS-GC/MS, respectively.
32168852	7	46	theme	cocos	1216:1220	arg1	fermentation					1197:1208	the fermentation	1193:1208	the fermentation of P. cocos	1193:1220	Our results suggested that it is applicable to produce polysaccharides and triterpenoids by the fermentation of P. cocos, and a strategy to improve triterpenoid production in the fermentation process was proposed.
32168852	6	47	theme	fermentation	980:991	arg1	media					993:997	the fermentation media	976:997	the fermentation media	976:997	Among the 62 compounds identified by HS-GC/MS analysis from the essential oil obtained from the fermentation media and a fresh external layer, the two main fragrances in common were linalool and methyl phenylacetate.
32168852	7	48	from	production	1262:1271	arg1	process					1293:1299	the fermentation process	1276:1299	the fermentation process	1276:1299	Our results suggested that it is applicable to produce polysaccharides and triterpenoids by the fermentation of P. cocos, and a strategy to improve triterpenoid production in the fermentation process was proposed.
32168852	2	49	theme	water-soluble	285:297	arg1	WPS					316:318	WPS	316:318	WPS	316:318	The main active components are water-soluble polysaccharides (WPS) and triterpenoids.
32168852	2	49	theme	water-soluble	285:297	arg1	components					270:279	The main active components	254:279	The main active components	254:279	The main active components are water-soluble polysaccharides (WPS) and triterpenoids.
32168852	2	49	theme	water-soluble	285:297	arg1	polysaccharides					299:313	water-soluble polysaccharides	285:313	water-soluble polysaccharides (WPS)	285:319	The main active components are water-soluble polysaccharides (WPS) and triterpenoids.
32168852	1	50	theme	edible	211:216	arg1	fungus					218:223	an important medicinal and edible fungus	184:223	an important medicinal and edible fungus	184:223	Poria cocos, an important medicinal and edible fungus, is well known in East Asia.
32168852	1	50	theme	edible	211:216	arg1	cocos					177:181	Poria cocos	171:181	Poria cocos	171:181	Poria cocos, an important medicinal and edible fungus, is well known in East Asia.
32168852	4	51	from	triterpenoids	561:573	arg1	mycelia					635:641	fermented mycelia	625:641	fermented mycelia	625:641	In this study, WPS, triterpenoids, monosaccharide composition, and essential oil in fermented mycelia and cultivated sclerotium were analyzed using UV spectrophotometry, HPLC, pre-column derivatization, and HS-GC/MS, respectively.
32168852	4	51	from	triterpenoids	561:573	arg1	sclerotium					658:667	cultivated sclerotium	647:667	cultivated sclerotium	647:667	In this study, WPS, triterpenoids, monosaccharide composition, and essential oil in fermented mycelia and cultivated sclerotium were analyzed using UV spectrophotometry, HPLC, pre-column derivatization, and HS-GC/MS, respectively.
32168852	1	52	theme	medicinal	197:205	arg1	fungus					218:223	an important medicinal and edible fungus	184:223	an important medicinal and edible fungus	184:223	Poria cocos, an important medicinal and edible fungus, is well known in East Asia.
32168852	1	52	theme	medicinal	197:205	arg1	cocos					177:181	Poria cocos	171:181	Poria cocos	171:181	Poria cocos, an important medicinal and edible fungus, is well known in East Asia.
32168852	4	53	theme	monosaccharide	576:589	arg1	composition					591:601	monosaccharide composition	576:601	monosaccharide composition	576:601	In this study, WPS, triterpenoids, monosaccharide composition, and essential oil in fermented mycelia and cultivated sclerotium were analyzed using UV spectrophotometry, HPLC, pre-column derivatization, and HS-GC/MS, respectively.
32168852	5	54	theme	several	837:843	arg1	WPS					800:802	WPS	800:802	WPS	800:802	Our results showed that the WPS and triterpenoids in mycelia are several times higher than those in sclerotium.
32168852	5	54	theme	several	837:843	arg1	times					845:849	several times	837:849	several times higher than those in sclerotium	837:881	Our results showed that the WPS and triterpenoids in mycelia are several times higher than those in sclerotium.
32168852	5	54	theme	several	837:843	arg1	triterpenoids					808:820	triterpenoids	808:820	triterpenoids	808:820	Our results showed that the WPS and triterpenoids in mycelia are several times higher than those in sclerotium.
32168852	0	55	theme	Essential	59:67	arg1	Oil					69:71	Essential Oil	59:71	Essential Oil	59:71	Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos.
32168852	5	56	from	triterpenoids	808:820	arg1	mycelia					825:831	mycelia	825:831	mycelia	825:831	Our results showed that the WPS and triterpenoids in mycelia are several times higher than those in sclerotium.
32168852	3	57	theme	P.	483:484	arg1	cocos					486:490	P. cocos	483:490	P. cocos	483:490	Due to the growing market demand, long cultivation period, and consumption of pine trunk during cultivation, alternative methods for producing P. cocos or its active components should be investigated.
33641944	1	0	theme	polysaccharides	280:294	arg1	fermentation					245:256	human fecal fermentation	233:256	human fecal fermentation	233:256	The in vitro simulated saliva-gastrointestinal digestion and human fecal fermentation of snow chrysanthemum polysaccharides (JHP) were investigated.
33641944	1	0	theme	polysaccharides	280:294	arg1	digestion					219:227	simulated saliva-gastrointestinal digestion	185:227	simulated saliva-gastrointestinal digestion	185:227	The in vitro simulated saliva-gastrointestinal digestion and human fecal fermentation of snow chrysanthemum polysaccharides (JHP) were investigated.
33641944	0	1	theme	in	91:92	arg1	digestion					100:108	in vitro digestion	91:108	in vitro digestion	91:108	Dynamic changes of structural characteristics of snow chrysanthemum polysaccharides during in vitro digestion and fecal fermentation and related impacts on gut microbiota.
33641944	4	2	theme	microbiota	1112:1121	arg1	abundance					1095:1103	abundance	1095:1103	abundance	1095:1103	Some beneficial bacteria, such as genera Bifidobacterium, Lactobacillus, Megamonas, and Megasphaera, significantly increased, suggesting that JHP-I could change the composition and abundance of gut microbiota.
33641944	4	2	theme	microbiota	1112:1121	arg1	composition					1079:1089	composition	1079:1089	composition	1079:1089	Some beneficial bacteria, such as genera Bifidobacterium, Lactobacillus, Megamonas, and Megasphaera, significantly increased, suggesting that JHP-I could change the composition and abundance of gut microbiota.
33641944	3	3	theme	indigestible	728:739	arg1	JHP					741:743	the indigestible JHP	724:743	the indigestible JHP (JHP-I)	724:751	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	3	3	theme	indigestible	728:739	arg1	JHP-I					746:750	JHP-I	746:750	JHP-I	746:750	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	5	4	theme	potential	1160:1168	arg1	source					1170:1175	a potential source	1158:1175	a potential source of prebiotics	1158:1189	These results suggest that JHP is a potential source of prebiotics, and can be helpful for better understanding of the potential digestion and fermentation mechanism of JHP.
33641944	5	4	theme	potential	1160:1168	arg1	JHP					1151:1153	JHP	1151:1153	JHP	1151:1153	These results suggest that JHP is a potential source of prebiotics, and can be helpful for better understanding of the potential digestion and fermentation mechanism of JHP.
33641944	4	5	theme	gut	1108:1110	arg1	microbiota					1112:1121	gut microbiota	1108:1121	gut microbiota	1108:1121	Some beneficial bacteria, such as genera Bifidobacterium, Lactobacillus, Megamonas, and Megasphaera, significantly increased, suggesting that JHP-I could change the composition and abundance of gut microbiota.
33641944	5	6	theme	potential	1243:1251	arg1	digestion					1253:1261	the potential digestion and fermentation mechanism	1239:1288	digestion	1253:1261	These results suggest that JHP is a potential source of prebiotics, and can be helpful for better understanding of the potential digestion and fermentation mechanism of JHP.
33641944	4	7	theme	beneficial	919:928	arg1	Bifidobacterium					955:969	genera Bifidobacterium	948:969	genera Bifidobacterium	948:969	Some beneficial bacteria, such as genera Bifidobacterium, Lactobacillus, Megamonas, and Megasphaera, significantly increased, suggesting that JHP-I could change the composition and abundance of gut microbiota.
33641944	4	7	theme	beneficial	919:928	arg1	bacteria					930:937	Some beneficial bacteria	914:937	Some beneficial bacteria	914:937	Some beneficial bacteria, such as genera Bifidobacterium, Lactobacillus, Megamonas, and Megasphaera, significantly increased, suggesting that JHP-I could change the composition and abundance of gut microbiota.
33641944	3	8	theme	in	590:591	arg1	fermentation					605:616	in vitro fecal fermentation	590:616	in vitro fecal fermentation	590:616	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	5	9	theme	digestion	1253:1261	arg1	understanding					1222:1234	better understanding	1215:1234	better understanding of the potential digestion and fermentation mechanism of JHP	1215:1295	These results suggest that JHP is a potential source of prebiotics, and can be helpful for better understanding of the potential digestion and fermentation mechanism of JHP.
33641944	2	10	theme	gastrointestinal	543:558	arg1	digestion					560:568	the gastrointestinal digestion	539:568	the gastrointestinal digestion	539:568	Results showed that reducing sugar contents of JHP increased during the gastrointestinal digestion, and glucose released with the decrease of its molecular weight, suggesting that JHP could be partially degraded under the gastrointestinal digestion.
33641944	1	11	theme	in	176:177	arg1	digestion					219:227	simulated saliva-gastrointestinal digestion	185:227	simulated saliva-gastrointestinal digestion	185:227	The in vitro simulated saliva-gastrointestinal digestion and human fecal fermentation of snow chrysanthemum polysaccharides (JHP) were investigated.
33641944	0	12	theme	fecal	114:118	arg1	fermentation					120:131	fecal fermentation	114:131	fecal fermentation	114:131	Dynamic changes of structural characteristics of snow chrysanthemum polysaccharides during in vitro digestion and fecal fermentation and related impacts on gut microbiota.
33641944	4	13	dep	composition	1079:1089	arg1	the					1075:1077	the	1075:1077	the	1075:1077	Some beneficial bacteria, such as genera Bifidobacterium, Lactobacillus, Megamonas, and Megasphaera, significantly increased, suggesting that JHP-I could change the composition and abundance of gut microbiota.
33641944	2	14	theme	weight	477:482	arg1	decrease					451:458	the decrease	447:458	the decrease of its molecular weight	447:482	Results showed that reducing sugar contents of JHP increased during the gastrointestinal digestion, and glucose released with the decrease of its molecular weight, suggesting that JHP could be partially degraded under the gastrointestinal digestion.
33641944	2	15	theme	sugar	350:354	arg1	contents					356:363	reducing sugar contents	341:363	reducing sugar contents of JHP	341:370	Results showed that reducing sugar contents of JHP increased during the gastrointestinal digestion, and glucose released with the decrease of its molecular weight, suggesting that JHP could be partially degraded under the gastrointestinal digestion.
33641944	1	16	theme	simulated	185:193	arg1	digestion					219:227	simulated saliva-gastrointestinal digestion	185:227	simulated saliva-gastrointestinal digestion	185:227	The in vitro simulated saliva-gastrointestinal digestion and human fecal fermentation of snow chrysanthemum polysaccharides (JHP) were investigated.
33641944	0	17	theme	Dynamic	0:6	arg1	changes					8:14	Dynamic changes	0:14	Dynamic changes of structural characteristics of snow chrysanthemum polysaccharides during in vitro digestion and fecal fermentation and related impacts on gut microbiota.	0:170	Dynamic changes of structural characteristics of snow chrysanthemum polysaccharides during in vitro digestion and fecal fermentation and related impacts on gut microbiota.
33641944	0	18	dep	in	91:92	arg1	vitro					94:98	vitro	94:98	vitro	94:98	Dynamic changes of structural characteristics of snow chrysanthemum polysaccharides during in vitro digestion and fecal fermentation and related impacts on gut microbiota.
33641944	1	19	theme	saliva-gastrointestinal	195:217	arg1	digestion					219:227	simulated saliva-gastrointestinal digestion	185:227	simulated saliva-gastrointestinal digestion	185:227	The in vitro simulated saliva-gastrointestinal digestion and human fecal fermentation of snow chrysanthemum polysaccharides (JHP) were investigated.
33641944	0	20	theme	structural	19:28	arg1	characteristics					30:44	structural characteristics	19:44	structural characteristics of snow chrysanthemum polysaccharides	19:82	Dynamic changes of structural characteristics of snow chrysanthemum polysaccharides during in vitro digestion and fecal fermentation and related impacts on gut microbiota.
33641944	3	21	theme	molar	644:648	arg1	ratio					650:654	molar ratio	644:654	molar ratio	644:654	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	0	22	theme	related	137:143	arg1	impacts					145:151	related impacts	137:151	related impacts on gut microbiota	137:169	Dynamic changes of structural characteristics of snow chrysanthemum polysaccharides during in vitro digestion and fecal fermentation and related impacts on gut microbiota.
33641944	2	23	theme	reducing	341:348	arg1	contents					356:363	reducing sugar contents	341:363	reducing sugar contents of JHP	341:370	Results showed that reducing sugar contents of JHP increased during the gastrointestinal digestion, and glucose released with the decrease of its molecular weight, suggesting that JHP could be partially degraded under the gastrointestinal digestion.
33641944	5	24	theme	better	1215:1220	arg1	understanding					1222:1234	better understanding	1215:1234	better understanding of the potential digestion and fermentation mechanism of JHP	1215:1295	These results suggest that JHP is a potential source of prebiotics, and can be helpful for better understanding of the potential digestion and fermentation mechanism of JHP.
33641944	3	25	theme	fecal	599:603	arg1	fermentation					605:616	in vitro fecal fermentation	590:616	in vitro fecal fermentation	590:616	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	5	26	theme	prebiotics	1180:1189	arg1	source					1170:1175	a potential source	1158:1175	a potential source of prebiotics	1158:1189	These results suggest that JHP is a potential source of prebiotics, and can be helpful for better understanding of the potential digestion and fermentation mechanism of JHP.
33641944	5	26	theme	prebiotics	1180:1189	arg1	JHP					1151:1153	JHP	1151:1153	JHP	1151:1153	These results suggest that JHP is a potential source of prebiotics, and can be helpful for better understanding of the potential digestion and fermentation mechanism of JHP.
33641944	5	27	theme	mechanism	1280:1288	arg1	understanding					1222:1234	better understanding	1215:1234	better understanding of the potential digestion and fermentation mechanism of JHP	1215:1295	These results suggest that JHP is a potential source of prebiotics, and can be helpful for better understanding of the potential digestion and fermentation mechanism of JHP.
33641944	1	28	theme	human	233:237	arg1	fermentation					245:256	human fecal fermentation	233:256	human fecal fermentation	233:256	The in vitro simulated saliva-gastrointestinal digestion and human fecal fermentation of snow chrysanthemum polysaccharides (JHP) were investigated.
33641944	0	29	theme	characteristics	30:44	arg1	changes					8:14	Dynamic changes	0:14	Dynamic changes of structural characteristics of snow chrysanthemum polysaccharides during in vitro digestion and fecal fermentation and related impacts on gut microbiota.	0:170	Dynamic changes of structural characteristics of snow chrysanthemum polysaccharides during in vitro digestion and fecal fermentation and related impacts on gut microbiota.
33641944	3	30	theme	monosaccharides	671:685	arg1	weight					633:638	the molecular weight	619:638	the molecular weight	619:638	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	3	30	theme	monosaccharides	671:685	arg1	ratio					650:654	molar ratio	644:654	molar ratio	644:654	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	2	31	theme	molecular	467:475	arg1	weight					477:482	its molecular weight	463:482	its molecular weight	463:482	Results showed that reducing sugar contents of JHP increased during the gastrointestinal digestion, and glucose released with the decrease of its molecular weight, suggesting that JHP could be partially degraded under the gastrointestinal digestion.
33641944	1	32	theme	fecal	239:243	arg1	fermentation					245:256	human fecal fermentation	233:256	human fecal fermentation	233:256	The in vitro simulated saliva-gastrointestinal digestion and human fecal fermentation of snow chrysanthemum polysaccharides (JHP) were investigated.
33641944	0	33	theme	chrysanthemum	54:66	arg1	polysaccharides					68:82	snow chrysanthemum polysaccharides	49:82	snow chrysanthemum polysaccharides	49:82	Dynamic changes of structural characteristics of snow chrysanthemum polysaccharides during in vitro digestion and fecal fermentation and related impacts on gut microbiota.
33641944	1	34	dep	in	176:177	arg1	vitro					179:183	vitro	179:183	vitro	179:183	The in vitro simulated saliva-gastrointestinal digestion and human fecal fermentation of snow chrysanthemum polysaccharides (JHP) were investigated.
33641944	0	35	theme	snow	49:52	arg1	polysaccharides					68:82	snow chrysanthemum polysaccharides	49:82	snow chrysanthemum polysaccharides	49:82	Dynamic changes of structural characteristics of snow chrysanthemum polysaccharides during in vitro digestion and fecal fermentation and related impacts on gut microbiota.
33641944	0	36	theme	gut	156:158	arg1	microbiota					160:169	gut microbiota	156:169	gut microbiota	156:169	Dynamic changes of structural characteristics of snow chrysanthemum polysaccharides during in vitro digestion and fecal fermentation and related impacts on gut microbiota.
33641944	0	37	from	fermentation	120:131	arg1	microbiota					160:169	gut microbiota	156:169	gut microbiota	156:169	Dynamic changes of structural characteristics of snow chrysanthemum polysaccharides during in vitro digestion and fecal fermentation and related impacts on gut microbiota.
33641944	3	38	theme	molecular	623:631	arg1	weight					633:638	the molecular weight	619:638	the molecular weight	619:638	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	2	39	theme	JHP	368:370	arg1	contents					356:363	reducing sugar contents	341:363	reducing sugar contents of JHP	341:370	Results showed that reducing sugar contents of JHP increased during the gastrointestinal digestion, and glucose released with the decrease of its molecular weight, suggesting that JHP could be partially degraded under the gastrointestinal digestion.
33641944	3	40	theme	gut	898:900	arg1	microbiota					902:911	gut microbiota	898:911	gut microbiota	898:911	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	5	41	theme	fermentation	1267:1278	arg1	mechanism					1280:1288	the potential digestion and fermentation mechanism	1239:1288	mechanism	1280:1288	These results suggest that JHP is a potential source of prebiotics, and can be helpful for better understanding of the potential digestion and fermentation mechanism of JHP.
33641944	4	42	theme	genera	948:953	arg1	Megamonas					987:995	Megamonas	987:995	Megamonas	987:995	Some beneficial bacteria, such as genera Bifidobacterium, Lactobacillus, Megamonas, and Megasphaera, significantly increased, suggesting that JHP-I could change the composition and abundance of gut microbiota.
33641944	4	42	theme	genera	948:953	arg1	Megasphaera					1002:1012	Megasphaera	1002:1012	Megasphaera	1002:1012	Some beneficial bacteria, such as genera Bifidobacterium, Lactobacillus, Megamonas, and Megasphaera, significantly increased, suggesting that JHP-I could change the composition and abundance of gut microbiota.
33641944	4	42	theme	genera	948:953	arg1	Lactobacillus					972:984	Lactobacillus	972:984	Lactobacillus	972:984	Some beneficial bacteria, such as genera Bifidobacterium, Lactobacillus, Megamonas, and Megasphaera, significantly increased, suggesting that JHP-I could change the composition and abundance of gut microbiota.
33641944	4	42	theme	genera	948:953	arg1	Bifidobacterium					955:969	genera Bifidobacterium	948:969	genera Bifidobacterium	948:969	Some beneficial bacteria, such as genera Bifidobacterium, Lactobacillus, Megamonas, and Megasphaera, significantly increased, suggesting that JHP-I could change the composition and abundance of gut microbiota.
33641944	3	43	dep	monosaccharides	671:685	arg1	galactose					688:696	galactose	688:696	galactose	688:696	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	3	43	dep	monosaccharides	671:685	arg1	acid					715:718	galacturonic acid	702:718	galacturonic acid	702:718	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	3	43	dep	monosaccharides	671:685	arg1	monosaccharides					671:685	constituent monosaccharides	659:685	constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I)	659:751	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	3	44	theme	galacturonic	702:713	arg1	acid					715:718	galacturonic acid	702:718	galacturonic acid	702:718	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	3	44	theme	galacturonic	702:713	arg1	monosaccharides					671:685	constituent monosaccharides	659:685	constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I)	659:751	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	3	45	theme	constituent	659:669	arg1	galactose					688:696	galactose	688:696	galactose	688:696	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	3	45	theme	constituent	659:669	arg1	acid					715:718	galacturonic acid	702:718	galacturonic acid	702:718	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	3	45	theme	constituent	659:669	arg1	monosaccharides					671:685	constituent monosaccharides	659:685	constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I)	659:751	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	3	46	dep	in	590:591	arg1	vitro					593:597	vitro	593:597	vitro	593:597	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	0	47	from	impacts	145:151	arg1	microbiota					160:169	gut microbiota	156:169	gut microbiota	156:169	Dynamic changes of structural characteristics of snow chrysanthemum polysaccharides during in vitro digestion and fecal fermentation and related impacts on gut microbiota.
33641944	0	48	from	digestion	100:108	arg1	microbiota					160:169	gut microbiota	156:169	gut microbiota	156:169	Dynamic changes of structural characteristics of snow chrysanthemum polysaccharides during in vitro digestion and fecal fermentation and related impacts on gut microbiota.
33641944	1	49	dep	digestion	219:227	arg1	The					172:174	The	172:174	The	172:174	The in vitro simulated saliva-gastrointestinal digestion and human fecal fermentation of snow chrysanthemum polysaccharides (JHP) were investigated.
33641944	1	50	theme	snow	261:264	arg1	JHP					297:299	JHP	297:299	JHP	297:299	The in vitro simulated saliva-gastrointestinal digestion and human fecal fermentation of snow chrysanthemum polysaccharides (JHP) were investigated.
33641944	1	50	theme	snow	261:264	arg1	polysaccharides					280:294	snow chrysanthemum polysaccharides	261:294	snow chrysanthemum polysaccharides (JHP)	261:300	The in vitro simulated saliva-gastrointestinal digestion and human fecal fermentation of snow chrysanthemum polysaccharides (JHP) were investigated.
33641944	0	51	theme	polysaccharides	68:82	arg1	characteristics					30:44	structural characteristics	19:44	structural characteristics of snow chrysanthemum polysaccharides	19:82	Dynamic changes of structural characteristics of snow chrysanthemum polysaccharides during in vitro digestion and fecal fermentation and related impacts on gut microbiota.
33641944	3	52	theme	JHP	741:743	arg1	galactose					688:696	galactose	688:696	galactose	688:696	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	3	52	theme	JHP	741:743	arg1	acid					715:718	galacturonic acid	702:718	galacturonic acid	702:718	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	3	52	theme	JHP	741:743	arg1	monosaccharides					671:685	constituent monosaccharides	659:685	constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I)	659:751	Furthermore, after in vitro fecal fermentation, the molecular weight and molar ratio of constituent monosaccharides (galactose and galacturonic acid) of the indigestible JHP (JHP-I) significantly decreased, and both monosaccharides and oligosaccharides released, suggesting that JHP-I could be further degraded and consumed by gut microbiota.
33641944	2	53	theme	gastrointestinal	393:408	arg1	digestion					410:418	the gastrointestinal digestion	389:418	the gastrointestinal digestion	389:418	Results showed that reducing sugar contents of JHP increased during the gastrointestinal digestion, and glucose released with the decrease of its molecular weight, suggesting that JHP could be partially degraded under the gastrointestinal digestion.
33641944	5	54	theme	JHP	1293:1295	arg1	digestion					1253:1261	the potential digestion and fermentation mechanism	1239:1288	digestion	1253:1261	These results suggest that JHP is a potential source of prebiotics, and can be helpful for better understanding of the potential digestion and fermentation mechanism of JHP.
33641944	5	54	theme	JHP	1293:1295	arg1	mechanism					1280:1288	the potential digestion and fermentation mechanism	1239:1288	mechanism	1280:1288	These results suggest that JHP is a potential source of prebiotics, and can be helpful for better understanding of the potential digestion and fermentation mechanism of JHP.
33641944	1	55	theme	chrysanthemum	266:278	arg1	JHP					297:299	JHP	297:299	JHP	297:299	The in vitro simulated saliva-gastrointestinal digestion and human fecal fermentation of snow chrysanthemum polysaccharides (JHP) were investigated.
33641944	1	55	theme	chrysanthemum	266:278	arg1	polysaccharides					280:294	snow chrysanthemum polysaccharides	261:294	snow chrysanthemum polysaccharides (JHP)	261:300	The in vitro simulated saliva-gastrointestinal digestion and human fecal fermentation of snow chrysanthemum polysaccharides (JHP) were investigated.
34057448	8	0	dep	assays	1907:1912	arg1	XTT					1915:1917	XTT	1915:1917	XTT	1915:1917	Over a period of 6-48 h, the FNC/alginate substrates did not adversely affect the viability of the BMMCs as determined by flow cytometry and microtiter assays (XTT and lactate dehydrogenase).
34057448	8	0	dep	assays	1907:1912	arg1	dehydrogenase					1931:1943	lactate dehydrogenase	1923:1943	lactate dehydrogenase	1923:1943	Over a period of 6-48 h, the FNC/alginate substrates did not adversely affect the viability of the BMMCs as determined by flow cytometry and microtiter assays (XTT and lactate dehydrogenase).
34057448	8	0	dep	assays	1907:1912	arg1	assays					1907:1912	microtiter assays	1896:1912	microtiter assays (XTT and lactate dehydrogenase)	1896:1944	Over a period of 6-48 h, the FNC/alginate substrates did not adversely affect the viability of the BMMCs as determined by flow cytometry and microtiter assays (XTT and lactate dehydrogenase).
34057448	7	1	theme	3D	1730:1731	arg1	bioprinter					1743:1752	a 3D extrusion bioprinter	1728:1752	a 3D extrusion bioprinter	1728:1752	The viability of BMMCs was also assessed following a time course exposure to hydrogel scaffolds that were fabricated from a commercial biomaterial ink composed of fibrillar nanocellulose (FNC) and sodium alginate using a 3D extrusion bioprinter.
34057448	9	2	theme	candidate	2042:2050	arg1	inks					2064:2067	candidate biomaterial inks	2042:2067	candidate biomaterial inks	2042:2067	This protocol describes an efficient method to rapidly screen the biochemical compatibility of candidate biomaterial inks for their utility as 3D scaffolds for post-print seeding with mast cells.
34057448	8	3	theme	h	1777:1777	arg1	period					1762:1767	a period	1760:1767	a period of 6-48 h	1760:1777	Over a period of 6-48 h, the FNC/alginate substrates did not adversely affect the viability of the BMMCs as determined by flow cytometry and microtiter assays (XTT and lactate dehydrogenase).
34057448	5	4	theme	propidium	1180:1188	arg1	PI					1198:1199	PI	1198:1199	PI	1198:1199	After 18 h of exposure to the CNC/agarose/D-mannitol matrix, BMMC viability was unaltered as measured by propidium iodide (PI) permeability.
34057448	5	4	theme	propidium	1180:1188	arg1	iodide					1190:1195	propidium iodide	1180:1195	propidium iodide (PI) permeability	1180:1213	After 18 h of exposure to the CNC/agarose/D-mannitol matrix, BMMC viability was unaltered as measured by propidium iodide (PI) permeability.
34057448	7	5	theme	sodium	1706:1711	arg1	alginate					1713:1720	sodium alginate	1706:1720	sodium alginate	1706:1720	The viability of BMMCs was also assessed following a time course exposure to hydrogel scaffolds that were fabricated from a commercial biomaterial ink composed of fibrillar nanocellulose (FNC) and sodium alginate using a 3D extrusion bioprinter.
34057448	9	6	theme	inks	2064:2067	arg1	compatibility					2025:2037	the biochemical compatibility	2009:2037	the biochemical compatibility of candidate biomaterial inks	2009:2067	This protocol describes an efficient method to rapidly screen the biochemical compatibility of candidate biomaterial inks for their utility as 3D scaffolds for post-print seeding with mast cells.
34057448	3	7	theme	culture	530:536	arg1	methods					538:544	Some 3D culture methods	522:544	Some 3D culture methods	522:544	Some 3D culture methods, including bioprinting, require that cells be embedded into a 3D matrix, making it difficult to extract and analyze the cells for changes in viability and biomarker expression without eliciting mechanical damage.
34057448	3	7	theme	culture	530:536	arg1	bioprinting					557:567	bioprinting	557:567	bioprinting	557:567	Some 3D culture methods, including bioprinting, require that cells be embedded into a 3D matrix, making it difficult to extract and analyze the cells for changes in viability and biomarker expression without eliciting mechanical damage.
34057448	2	8	theme	many	304:307	arg1	inks					321:324	many biomaterial inks	304:324	many biomaterial inks	304:324	Because many biomaterial inks can be potentially cytotoxic to primary cells, it is necessary to determine the biocompatibility of these hydrogel composites prior to their utilization in costly 3D tissue engineering processes.
34057448	7	9	theme	time	1562:1565	arg1	course					1567:1572	a time course	1560:1572	a time course exposure to hydrogel scaffolds that were fabricated from a commercial biomaterial ink composed of fibrillar nanocellulose (FNC) and sodium alginate using a 3D extrusion bioprinter	1560:1752	The viability of BMMCs was also assessed following a time course exposure to hydrogel scaffolds that were fabricated from a commercial biomaterial ink composed of fibrillar nanocellulose (FNC) and sodium alginate using a 3D extrusion bioprinter.
34057448	4	10	theme	composite	898:906	arg1	biocompatibility					827:842	the biocompatibility	823:842	the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs)	823:999	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	8	11	theme	lactate	1923:1929	arg1	dehydrogenase					1931:1943	lactate dehydrogenase	1923:1943	lactate dehydrogenase	1923:1943	Over a period of 6-48 h, the FNC/alginate substrates did not adversely affect the viability of the BMMCs as determined by flow cytometry and microtiter assays (XTT and lactate dehydrogenase).
34057448	8	11	theme	lactate	1923:1929	arg1	assays					1907:1912	microtiter assays	1896:1912	microtiter assays (XTT and lactate dehydrogenase)	1896:1944	Over a period of 6-48 h, the FNC/alginate substrates did not adversely affect the viability of the BMMCs as determined by flow cytometry and microtiter assays (XTT and lactate dehydrogenase).
34057448	3	12	from	changes	676:682	arg1	viability					687:695	viability	687:695	viability	687:695	Some 3D culture methods, including bioprinting, require that cells be embedded into a 3D matrix, making it difficult to extract and analyze the cells for changes in viability and biomarker expression without eliciting mechanical damage.
34057448	3	12	from	changes	676:682	arg1	expression					711:720	biomarker expression	701:720	biomarker expression	701:720	Some 3D culture methods, including bioprinting, require that cells be embedded into a 3D matrix, making it difficult to extract and analyze the cells for changes in viability and biomarker expression without eliciting mechanical damage.
34057448	9	13	theme	post-print	2107:2116	arg1	seeding					2118:2124	post-print seeding	2107:2124	post-print seeding with mast cells	2107:2140	This protocol describes an efficient method to rapidly screen the biochemical compatibility of candidate biomaterial inks for their utility as 3D scaffolds for post-print seeding with mast cells.
34057448	6	14	from	composite	1498:1506	arg1	CNC					1480:1482	CNC	1480:1482	CNC in the bioink composite	1480:1506	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	6	14	from	composite	1498:1506	arg1	amount					1470:1475	the amount	1466:1475	the amount of CNC in the bioink composite	1466:1506	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	7	15	theme	BMMCs	1526:1530	arg1	viability					1513:1521	The viability	1509:1521	The viability of BMMCs	1509:1530	The viability of BMMCs was also assessed following a time course exposure to hydrogel scaffolds that were fabricated from a commercial biomaterial ink composed of fibrillar nanocellulose (FNC) and sodium alginate using a 3D extrusion bioprinter.
34057448	4	16	theme	embedded	881:888	arg1	composite					898:906	a crystalline nanocellulose (CNC) embedded agarose composite	847:906	a crystalline nanocellulose (CNC) embedded agarose composite	847:906	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	6	17	theme	CNC/agarose/D-mannitol	1247:1268	arg1	substrate					1270:1278	the CNC/agarose/D-mannitol substrate	1243:1278	the CNC/agarose/D-mannitol substrate	1243:1278	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	6	18	theme	bioink	1491:1496	arg1	composite					1498:1506	the bioink composite	1487:1506	the bioink composite	1487:1506	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	2	19	theme	tissue	492:497	arg1	processes					511:519	costly 3D tissue engineering processes	482:519	costly 3D tissue engineering processes	482:519	Because many biomaterial inks can be potentially cytotoxic to primary cells, it is necessary to determine the biocompatibility of these hydrogel composites prior to their utilization in costly 3D tissue engineering processes.
34057448	0	20	theme	Ink	72:74	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture.	0:117	Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture.
34057448	4	21	theme	mouse	956:960	arg1	BMMCs					994:998	BMMCs	994:998	BMMCs	994:998	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	4	21	theme	mouse	956:960	arg1	cells					987:991	mouse bone marrow-derived mast cells	956:991	mouse bone marrow-derived mast cells (BMMCs)	956:999	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	6	22	from	CNC	1480:1482	arg1	composite					1498:1506	the bioink composite	1487:1506	the bioink composite	1487:1506	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	7	23	theme	commercial	1633:1642	arg1	ink					1656:1658	a commercial biomaterial ink	1631:1658	a commercial biomaterial ink composed of fibrillar nanocellulose (FNC) and sodium alginate using a 3D extrusion bioprinter	1631:1752	The viability of BMMCs was also assessed following a time course exposure to hydrogel scaffolds that were fabricated from a commercial biomaterial ink composed of fibrillar nanocellulose (FNC) and sodium alginate using a 3D extrusion bioprinter.
34057448	2	24	theme	costly	482:487	arg1	processes					511:519	costly 3D tissue engineering processes	482:519	costly 3D tissue engineering processes	482:519	Because many biomaterial inks can be potentially cytotoxic to primary cells, it is necessary to determine the biocompatibility of these hydrogel composites prior to their utilization in costly 3D tissue engineering processes.
34057448	6	25	theme	receptor	1352:1359	arg1	expression					1316:1325	their expression	1310:1325	their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117)	1310:1414	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	5	26	theme	exposure	1089:1096	arg1	h					1084:1084	18 h	1081:1084	18 h of exposure to the CNC/agarose/D-mannitol matrix	1081:1133	After 18 h of exposure to the CNC/agarose/D-mannitol matrix, BMMC viability was unaltered as measured by propidium iodide (PI) permeability.
34057448	9	27	with	seeding	2118:2124	arg1	cells					2136:2140	mast cells	2131:2140	mast cells	2131:2140	This protocol describes an efficient method to rapidly screen the biochemical compatibility of candidate biomaterial inks for their utility as 3D scaffolds for post-print seeding with mast cells.
34057448	0	28	theme	Mast	100:103	arg1	Culture					110:116	Bone Marrow-Derived Mast Cell Culture	80:116	Bone Marrow-Derived Mast Cell Culture	80:116	Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture.
34057448	4	29	theme	culture	935:941	arg1	system					943:948	a 24-well culture system	925:948	a 24-well culture system	925:948	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	1	30	dep	Three-dimensional	119:135	arg1	3D					138:139	3D	138:139	3D	138:139	Three-dimensional (3D) bioprinting utilizes hydrogel-based composites (or biomaterial inks) that are deposited in a pattern, forming a substrate onto which cells are deposited.
34057448	8	31	theme	microtiter	1896:1905	arg1	XTT					1915:1917	XTT	1915:1917	XTT	1915:1917	Over a period of 6-48 h, the FNC/alginate substrates did not adversely affect the viability of the BMMCs as determined by flow cytometry and microtiter assays (XTT and lactate dehydrogenase).
34057448	8	31	theme	microtiter	1896:1905	arg1	dehydrogenase					1931:1943	lactate dehydrogenase	1923:1943	lactate dehydrogenase	1923:1943	Over a period of 6-48 h, the FNC/alginate substrates did not adversely affect the viability of the BMMCs as determined by flow cytometry and microtiter assays (XTT and lactate dehydrogenase).
34057448	8	31	theme	microtiter	1896:1905	arg1	assays					1907:1912	microtiter assays	1896:1912	microtiter assays (XTT and lactate dehydrogenase)	1896:1944	Over a period of 6-48 h, the FNC/alginate substrates did not adversely affect the viability of the BMMCs as determined by flow cytometry and microtiter assays (XTT and lactate dehydrogenase).
34057448	0	32	theme	Embedded	43:50	arg1	Ink					72:74	a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink	15:74	a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink	15:74	Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture.
34057448	6	33	from	amount	1470:1475	arg1	composite					1498:1506	the bioink composite	1487:1506	the bioink composite	1487:1506	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	2	34	theme	composites	441:450	arg1	biocompatibility					406:421	the biocompatibility	402:421	the biocompatibility of these hydrogel composites prior to their utilization in costly 3D tissue engineering processes	402:519	Because many biomaterial inks can be potentially cytotoxic to primary cells, it is necessary to determine the biocompatibility of these hydrogel composites prior to their utilization in costly 3D tissue engineering processes.
34057448	4	35	link	marrow-derived	967:980	arg1	BMMCs					994:998	BMMCs	994:998	BMMCs	994:998	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	4	35	link	marrow-derived	967:980	arg1	cells					987:991	mouse bone marrow-derived mast cells	956:991	mouse bone marrow-derived mast cells (BMMCs)	956:999	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	9	36	theme	biomaterial	2052:2062	arg1	inks					2064:2067	candidate biomaterial inks	2042:2067	candidate biomaterial inks	2042:2067	This protocol describes an efficient method to rapidly screen the biochemical compatibility of candidate biomaterial inks for their utility as 3D scaffolds for post-print seeding with mast cells.
34057448	5	37	theme	BMMC	1136:1139	arg1	viability					1141:1149	BMMC viability	1136:1149	BMMC viability	1136:1149	After 18 h of exposure to the CNC/agarose/D-mannitol matrix, BMMC viability was unaltered as measured by propidium iodide (PI) permeability.
34057448	6	38	theme	factor	1387:1392	arg1	receptor					1394:1401	the stem cell factor receptor	1373:1401	the stem cell factor receptor (Kit; CD117)	1373:1414	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	6	38	theme	factor	1387:1392	arg1	Kit					1404:1406	Kit	1404:1406	Kit	1404:1406	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	7	39	theme	course	1567:1572	arg1	exposure					1574:1581	a time course exposure	1560:1581	a time course exposure to hydrogel scaffolds that were fabricated from a commercial biomaterial ink composed of fibrillar nanocellulose (FNC) and sodium alginate using a 3D extrusion bioprinter	1560:1752	The viability of BMMCs was also assessed following a time course exposure to hydrogel scaffolds that were fabricated from a commercial biomaterial ink composed of fibrillar nanocellulose (FNC) and sodium alginate using a 3D extrusion bioprinter.
34057448	4	40	theme	marrow-derived	967:980	arg1	BMMCs					994:998	BMMCs	994:998	BMMCs	994:998	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	4	40	theme	marrow-derived	967:980	arg1	cells					987:991	mouse bone marrow-derived mast cells	956:991	mouse bone marrow-derived mast cells (BMMCs)	956:999	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	6	41	theme	stem	1377:1380	arg1	receptor					1394:1401	the stem cell factor receptor	1373:1401	the stem cell factor receptor (Kit; CD117)	1373:1414	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	6	41	theme	stem	1377:1380	arg1	Kit					1404:1406	Kit	1404:1406	Kit	1404:1406	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	0	42	theme	Nanocellulose	29:41	arg1	Ink					72:74	a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink	15:74	a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink	15:74	Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture.
34057448	9	43	theme	efficient	1974:1982	arg1	method					1984:1989	an efficient method	1971:1989	an efficient method to rapidly screen the biochemical compatibility of candidate biomaterial inks for their utility as 3D scaffolds for post-print seeding with mast cells	1971:2140	This protocol describes an efficient method to rapidly screen the biochemical compatibility of candidate biomaterial inks for their utility as 3D scaffolds for post-print seeding with mast cells.
34057448	0	44	theme	Agarose	52:58	arg1	Ink					72:74	a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink	15:74	a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink	15:74	Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture.
34057448	5	45	theme	iodide	1190:1195	arg1	permeability					1202:1213	propidium iodide (PI) permeability	1180:1213	propidium iodide (PI) permeability	1180:1213	After 18 h of exposure to the CNC/agarose/D-mannitol matrix, BMMC viability was unaltered as measured by propidium iodide (PI) permeability.
34057448	7	46	theme	extrusion	1733:1741	arg1	bioprinter					1743:1752	a 3D extrusion bioprinter	1728:1752	a 3D extrusion bioprinter	1728:1752	The viability of BMMCs was also assessed following a time course exposure to hydrogel scaffolds that were fabricated from a commercial biomaterial ink composed of fibrillar nanocellulose (FNC) and sodium alginate using a 3D extrusion bioprinter.
34057448	4	47	theme	biomarker	1053:1061	arg1	expression					1063:1072	biomarker expression	1053:1072	biomarker expression	1053:1072	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	6	48	theme	receptor	1394:1401	arg1	expression					1316:1325	their expression	1310:1325	their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117)	1310:1414	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	4	49	with	biocompatibility	827:842	arg1	BMMCs					994:998	BMMCs	994:998	BMMCs	994:998	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	4	49	with	biocompatibility	827:842	arg1	cells					987:991	mouse bone marrow-derived mast cells	956:991	mouse bone marrow-derived mast cells (BMMCs)	956:999	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	4	50	theme	cytometric	1012:1021	arg1	assays					1023:1028	flow cytometric assays	1007:1028	flow cytometric assays for cell viability and biomarker expression	1007:1072	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	9	51	theme	biochemical	2013:2023	arg1	compatibility					2025:2037	the biochemical compatibility	2009:2037	the biochemical compatibility of candidate biomaterial inks	2009:2067	This protocol describes an efficient method to rapidly screen the biochemical compatibility of candidate biomaterial inks for their utility as 3D scaffolds for post-print seeding with mast cells.
34057448	3	52	theme	biomarker	701:709	arg1	expression					711:720	biomarker expression	701:720	biomarker expression	701:720	Some 3D culture methods, including bioprinting, require that cells be embedded into a 3D matrix, making it difficult to extract and analyze the cells for changes in viability and biomarker expression without eliciting mechanical damage.
34057448	8	53	theme	FNC/alginate	1784:1795	arg1	substrates					1797:1806	the FNC/alginate substrates	1780:1806	the FNC/alginate substrates	1780:1806	Over a period of 6-48 h, the FNC/alginate substrates did not adversely affect the viability of the BMMCs as determined by flow cytometry and microtiter assays (XTT and lactate dehydrogenase).
34057448	3	54	theme	3D	527:528	arg1	methods					538:544	Some 3D culture methods	522:544	Some 3D culture methods	522:544	Some 3D culture methods, including bioprinting, require that cells be embedded into a 3D matrix, making it difficult to extract and analyze the cells for changes in viability and biomarker expression without eliciting mechanical damage.
34057448	3	54	theme	3D	527:528	arg1	bioprinting					557:567	bioprinting	557:567	bioprinting	557:567	Some 3D culture methods, including bioprinting, require that cells be embedded into a 3D matrix, making it difficult to extract and analyze the cells for changes in viability and biomarker expression without eliciting mechanical damage.
34057448	2	55	theme	biomaterial	309:319	arg1	inks					321:324	many biomaterial inks	304:324	many biomaterial inks	304:324	Because many biomaterial inks can be potentially cytotoxic to primary cells, it is necessary to determine the biocompatibility of these hydrogel composites prior to their utilization in costly 3D tissue engineering processes.
34057448	1	56	theme	biomaterial	193:203	arg1	inks					205:208	biomaterial inks	193:208	biomaterial inks	193:208	Three-dimensional (3D) bioprinting utilizes hydrogel-based composites (or biomaterial inks) that are deposited in a pattern, forming a substrate onto which cells are deposited.
34057448	3	57	theme	mechanical	740:749	arg1	damage					751:756	mechanical damage	740:756	mechanical damage	740:756	Some 3D culture methods, including bioprinting, require that cells be embedded into a 3D matrix, making it difficult to extract and analyze the cells for changes in viability and biomarker expression without eliciting mechanical damage.
34057448	4	58	theme	agarose	890:896	arg1	composite					898:906	a crystalline nanocellulose (CNC) embedded agarose composite	847:906	a crystalline nanocellulose (CNC) embedded agarose composite	847:906	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	9	59	theme	3D	2090:2091	arg1	scaffolds					2093:2101	3D scaffolds	2090:2101	3D scaffolds	2090:2101	This protocol describes an efficient method to rapidly screen the biochemical compatibility of candidate biomaterial inks for their utility as 3D scaffolds for post-print seeding with mast cells.
34057448	6	60	theme	IgE	1348:1350	arg1	FcεRI					1362:1366	FcεRI	1362:1366	FcεRI	1362:1366	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	6	60	theme	IgE	1348:1350	arg1	receptor					1352:1359	the high-affinity IgE receptor	1330:1359	the high-affinity IgE receptor (FcεRI)	1330:1367	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	1	61	theme	Three-dimensional	119:135	arg1	bioprinting					142:152	Three-dimensional (3D) bioprinting	119:152	Three-dimensional (3D) bioprinting	119:152	Three-dimensional (3D) bioprinting utilizes hydrogel-based composites (or biomaterial inks) that are deposited in a pattern, forming a substrate onto which cells are deposited.
34057448	8	62	theme	BMMCs	1854:1858	arg1	viability					1837:1845	the viability	1833:1845	the viability of the BMMCs	1833:1858	Over a period of 6-48 h, the FNC/alginate substrates did not adversely affect the viability of the BMMCs as determined by flow cytometry and microtiter assays (XTT and lactate dehydrogenase).
34057448	3	63	theme	3D	608:609	arg1	matrix					611:616	a 3D matrix	606:616	a 3D matrix	606:616	Some 3D culture methods, including bioprinting, require that cells be embedded into a 3D matrix, making it difficult to extract and analyze the cells for changes in viability and biomarker expression without eliciting mechanical damage.
34057448	7	64	theme	fibrillar	1672:1680	arg1	FNC					1697:1699	FNC	1697:1699	FNC	1697:1699	The viability of BMMCs was also assessed following a time course exposure to hydrogel scaffolds that were fabricated from a commercial biomaterial ink composed of fibrillar nanocellulose (FNC) and sodium alginate using a 3D extrusion bioprinter.
34057448	7	64	theme	fibrillar	1672:1680	arg1	nanocellulose					1682:1694	fibrillar nanocellulose	1672:1694	fibrillar nanocellulose (FNC)	1672:1700	The viability of BMMCs was also assessed following a time course exposure to hydrogel scaffolds that were fabricated from a commercial biomaterial ink composed of fibrillar nanocellulose (FNC) and sodium alginate using a 3D extrusion bioprinter.
34057448	9	65	theme	mast	2131:2134	arg1	cells					2136:2140	mast cells	2131:2140	mast cells	2131:2140	This protocol describes an efficient method to rapidly screen the biochemical compatibility of candidate biomaterial inks for their utility as 3D scaffolds for post-print seeding with mast cells.
34057448	2	66	theme	3D	489:490	arg1	processes					511:519	costly 3D tissue engineering processes	482:519	costly 3D tissue engineering processes	482:519	Because many biomaterial inks can be potentially cytotoxic to primary cells, it is necessary to determine the biocompatibility of these hydrogel composites prior to their utilization in costly 3D tissue engineering processes.
34057448	6	67	theme	high-affinity	1334:1346	arg1	FcεRI					1362:1366	FcεRI	1362:1366	FcεRI	1362:1366	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	6	67	theme	high-affinity	1334:1346	arg1	receptor					1352:1359	the high-affinity IgE receptor	1330:1359	the high-affinity IgE receptor (FcεRI)	1330:1367	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	0	68	theme	Marrow-Derived	85:98	arg1	Culture					110:116	Bone Marrow-Derived Mast Cell Culture	80:116	Bone Marrow-Derived Mast Cell Culture	80:116	Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture.
34057448	6	69	theme	CNC	1480:1482	arg1	CNC					1480:1482	CNC	1480:1482	CNC in the bioink composite	1480:1506	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	6	69	theme	CNC	1480:1482	arg1	amount					1470:1475	the amount	1466:1475	the amount of CNC in the bioink composite	1466:1506	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	1	70	theme	hydrogel-based	163:176	arg1	composites					178:187	hydrogel-based composites	163:187	hydrogel-based composites	163:187	Three-dimensional (3D) bioprinting utilizes hydrogel-based composites (or biomaterial inks) that are deposited in a pattern, forming a substrate onto which cells are deposited.
34057448	7	71	theme	biomaterial	1644:1654	arg1	ink					1656:1658	a commercial biomaterial ink	1631:1658	a commercial biomaterial ink composed of fibrillar nanocellulose (FNC) and sodium alginate using a 3D extrusion bioprinter	1631:1752	The viability of BMMCs was also assessed following a time course exposure to hydrogel scaffolds that were fabricated from a commercial biomaterial ink composed of fibrillar nanocellulose (FNC) and sodium alginate using a 3D extrusion bioprinter.
34057448	0	72	theme	Cell	105:108	arg1	Culture					110:116	Bone Marrow-Derived Mast Cell Culture	80:116	Bone Marrow-Derived Mast Cell Culture	80:116	Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture.
34057448	2	73	theme	engineering	499:509	arg1	processes					511:519	costly 3D tissue engineering processes	482:519	costly 3D tissue engineering processes	482:519	Because many biomaterial inks can be potentially cytotoxic to primary cells, it is necessary to determine the biocompatibility of these hydrogel composites prior to their utilization in costly 3D tissue engineering processes.
34057448	2	74	from	utilization	467:477	arg1	processes					511:519	costly 3D tissue engineering processes	482:519	costly 3D tissue engineering processes	482:519	Because many biomaterial inks can be potentially cytotoxic to primary cells, it is necessary to determine the biocompatibility of these hydrogel composites prior to their utilization in costly 3D tissue engineering processes.
34057448	2	75	theme	hydrogel	432:439	arg1	composites					441:450	these hydrogel composites	426:450	these hydrogel composites	426:450	Because many biomaterial inks can be potentially cytotoxic to primary cells, it is necessary to determine the biocompatibility of these hydrogel composites prior to their utilization in costly 3D tissue engineering processes.
34057448	0	76	theme	Crystalline	17:27	arg1	Ink					72:74	a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink	15:74	a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink	15:74	Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture.
34057448	7	77	theme	hydrogel	1586:1593	arg1	scaffolds					1595:1603	hydrogel scaffolds	1586:1603	hydrogel scaffolds that were fabricated from a commercial biomaterial ink composed of fibrillar nanocellulose (FNC) and sodium alginate using a 3D extrusion bioprinter	1586:1752	The viability of BMMCs was also assessed following a time course exposure to hydrogel scaffolds that were fabricated from a commercial biomaterial ink composed of fibrillar nanocellulose (FNC) and sodium alginate using a 3D extrusion bioprinter.
34057448	6	78	theme	cell	1382:1385	arg1	receptor					1394:1401	the stem cell factor receptor	1373:1401	the stem cell factor receptor (Kit; CD117)	1373:1414	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	6	78	theme	cell	1382:1385	arg1	Kit					1404:1406	Kit	1404:1406	Kit	1404:1406	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	4	79	theme	bone	962:965	arg1	BMMCs					994:998	BMMCs	994:998	BMMCs	994:998	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	4	79	theme	bone	962:965	arg1	cells					987:991	mouse bone marrow-derived mast cells	956:991	mouse bone marrow-derived mast cells (BMMCs)	956:999	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	4	80	theme	concept	795:801	arg1	proof					786:790	proof	786:790	proof of concept	786:801	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	8	81	theme	flow	1877:1880	arg1	cytometry					1882:1890	flow cytometry	1877:1890	flow cytometry	1877:1890	Over a period of 6-48 h, the FNC/alginate substrates did not adversely affect the viability of the BMMCs as determined by flow cytometry and microtiter assays (XTT and lactate dehydrogenase).
34057448	4	82	theme	24-well	927:933	arg1	system					943:948	a 24-well culture system	925:948	a 24-well culture system	925:948	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	0	83	theme	Biomaterial	60:70	arg1	Ink					72:74	a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink	15:74	a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink	15:74	Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture.
34057448	5	84	theme	CNC/agarose/D-mannitol	1105:1126	arg1	matrix					1128:1133	the CNC/agarose/D-mannitol matrix	1101:1133	the CNC/agarose/D-mannitol matrix	1101:1133	After 18 h of exposure to the CNC/agarose/D-mannitol matrix, BMMC viability was unaltered as measured by propidium iodide (PI) permeability.
34057448	4	85	theme	mast	982:985	arg1	BMMCs					994:998	BMMCs	994:998	BMMCs	994:998	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	4	85	theme	mast	982:985	arg1	cells					987:991	mouse bone marrow-derived mast cells	956:991	mouse bone marrow-derived mast cells (BMMCs)	956:999	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	6	86	dep	Kit	1404:1406	arg1	CD117					1409:1413	CD117	1409:1413	CD117	1409:1413	However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcεRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite.
34057448	4	87	theme	cell	1034:1037	arg1	viability					1039:1047	cell viability	1034:1047	cell viability	1034:1047	This protocol describes as proof of concept, a method to assess the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated into a 24-well culture system, with mouse bone marrow-derived mast cells (BMMCs) using flow cytometric assays for cell viability and biomarker expression.
34057448	2	88	theme	primary	358:364	arg1	cells					366:370	primary cells	358:370	primary cells	358:370	Because many biomaterial inks can be potentially cytotoxic to primary cells, it is necessary to determine the biocompatibility of these hydrogel composites prior to their utilization in costly 3D tissue engineering processes.
31952612	4	0	theme	starch	598:603	arg1	particles					605:613	the microporous starch particles	582:613	the microporous starch particles within the hierarchical system	582:644	Thrombin was evenly and independently distributed on the microporous starch particles within the hierarchical system and served to accelerate hemostasis.
31952612	5	1	theme	high	755:758	arg1	potential					765:773	high zeta potential	755:773	high zeta potential	755:773	Meanwhile, the composite displayed excellent water absorption capacity, high zeta potential, roughness, and porosity.
31952612	3	2	theme	porous	409:414	arg1	structure					416:424	a hierarchical porous structure	394:424	a hierarchical porous structure system consisting of porous and rough structures with evenly distributed microporous starch particles	394:526	The composite showed a hierarchical porous structure system consisting of porous and rough structures with evenly distributed microporous starch particles.
31952612	4	3	theme	microporous	586:596	arg1	particles					605:613	the microporous starch particles	582:613	the microporous starch particles within the hierarchical system	582:644	Thrombin was evenly and independently distributed on the microporous starch particles within the hierarchical system and served to accelerate hemostasis.
31952612	5	4	theme	zeta	760:763	arg1	potential					765:773	high zeta potential	755:773	high zeta potential	755:773	Meanwhile, the composite displayed excellent water absorption capacity, high zeta potential, roughness, and porosity.
31952612	7	5	theme	wound	1039:1043	arg1	hemorrhage					1045:1054	wound hemorrhage	1039:1054	wound hemorrhage	1039:1054	Experiments using animal models demonstrated that the composite could effectively control wound hemorrhage.
31952612	2	6	theme	starch	332:337	arg1	particles					339:347	thrombin-occupied microporous starch particles	302:347	thrombin-occupied microporous starch particles	302:347	In this study, we produced a puff pastry-like chitosan/konjac glucomannan matrix loaded with thrombin-occupied microporous starch particles to initiate hemostasis.
31952612	3	7	theme	structure	416:424	arg1	system					426:431	a hierarchical porous structure system	394:431	a hierarchical porous structure system consisting of porous and rough structures with evenly distributed microporous starch particles	394:526	The composite showed a hierarchical porous structure system consisting of porous and rough structures with evenly distributed microporous starch particles.
31952612	8	8	theme	effective	1117:1125	arg1	degradability					1127:1139	effective degradability	1117:1139	effective degradability	1117:1139	Furthermore, the composite showed good biocompatibility and effective degradability.
31952612	6	9	theme	thrombin	887:894	arg1	APTT					902:905	APTT	902:905	APTT	902:905	The composite was found to elicit desirable pro-thrombin time (PT), activated partial thrombin time (APTT), and whole blood clotting indices (BCI).
31952612	6	9	theme	thrombin	887:894	arg1	time					896:899	activated partial thrombin time	869:899	activated partial thrombin time (APTT)	869:906	The composite was found to elicit desirable pro-thrombin time (PT), activated partial thrombin time (APTT), and whole blood clotting indices (BCI).
31952612	2	10	theme	microporous	320:330	arg1	particles					339:347	thrombin-occupied microporous starch particles	302:347	thrombin-occupied microporous starch particles	302:347	In this study, we produced a puff pastry-like chitosan/konjac glucomannan matrix loaded with thrombin-occupied microporous starch particles to initiate hemostasis.
31952612	5	11	theme	excellent	718:726	arg1	capacity					745:752	excellent water absorption capacity	718:752	excellent water absorption capacity	718:752	Meanwhile, the composite displayed excellent water absorption capacity, high zeta potential, roughness, and porosity.
31952612	6	12	theme	partial	879:885	arg1	APTT					902:905	APTT	902:905	APTT	902:905	The composite was found to elicit desirable pro-thrombin time (PT), activated partial thrombin time (APTT), and whole blood clotting indices (BCI).
31952612	6	12	theme	partial	879:885	arg1	time					896:899	activated partial thrombin time	869:899	activated partial thrombin time (APTT)	869:906	The composite was found to elicit desirable pro-thrombin time (PT), activated partial thrombin time (APTT), and whole blood clotting indices (BCI).
31952612	2	13	theme	thrombin-occupied	302:318	arg1	particles					339:347	thrombin-occupied microporous starch particles	302:347	thrombin-occupied microporous starch particles	302:347	In this study, we produced a puff pastry-like chitosan/konjac glucomannan matrix loaded with thrombin-occupied microporous starch particles to initiate hemostasis.
31952612	1	14	theme	severe	194:199	arg1	trauma					201:206	severe trauma	194:206	severe trauma	194:206	Hemorrhage control is key for reducing mortality following severe trauma.
31952612	3	15	theme	hierarchical	396:407	arg1	structure					416:424	a hierarchical porous structure	394:424	a hierarchical porous structure system consisting of porous and rough structures with evenly distributed microporous starch particles	394:526	The composite showed a hierarchical porous structure system consisting of porous and rough structures with evenly distributed microporous starch particles.
31952612	5	16	theme	water	728:732	arg1	capacity					745:752	excellent water absorption capacity	718:752	excellent water absorption capacity	718:752	Meanwhile, the composite displayed excellent water absorption capacity, high zeta potential, roughness, and porosity.
31952612	6	17	theme	activated	869:877	arg1	APTT					902:905	APTT	902:905	APTT	902:905	The composite was found to elicit desirable pro-thrombin time (PT), activated partial thrombin time (APTT), and whole blood clotting indices (BCI).
31952612	6	17	theme	activated	869:877	arg1	time					896:899	activated partial thrombin time	869:899	activated partial thrombin time (APTT)	869:906	The composite was found to elicit desirable pro-thrombin time (PT), activated partial thrombin time (APTT), and whole blood clotting indices (BCI).
31952612	0	18	theme	Puff	0:3	arg1	chitosan/konjac					17:31	Puff pastry-like chitosan/konjac	0:31	Puff pastry-like chitosan/konjac	0:31	Puff pastry-like chitosan/konjac glucomannan matrix with thrombin-occupied microporous starch particles as a composite for hemostasis.
31952612	3	19	theme	starch	511:516	arg1	particles					518:526	evenly distributed microporous starch particles	480:526	evenly distributed microporous starch particles	480:526	The composite showed a hierarchical porous structure system consisting of porous and rough structures with evenly distributed microporous starch particles.
31952612	0	20	theme	pastry-like	5:15	arg1	chitosan/konjac					17:31	Puff pastry-like chitosan/konjac	0:31	Puff pastry-like chitosan/konjac	0:31	Puff pastry-like chitosan/konjac glucomannan matrix with thrombin-occupied microporous starch particles as a composite for hemostasis.
31952612	5	21	theme	absorption	734:743	arg1	capacity					745:752	excellent water absorption capacity	718:752	excellent water absorption capacity	718:752	Meanwhile, the composite displayed excellent water absorption capacity, high zeta potential, roughness, and porosity.
31952612	3	22	theme	porous	447:452	arg1	structures					464:473	porous and rough structures	447:473	porous and rough structures with evenly distributed microporous starch particles	447:526	The composite showed a hierarchical porous structure system consisting of porous and rough structures with evenly distributed microporous starch particles.
31952612	8	23	theme	good	1091:1094	arg1	biocompatibility					1096:1111	good biocompatibility	1091:1111	good biocompatibility	1091:1111	Furthermore, the composite showed good biocompatibility and effective degradability.
31952612	0	24	theme	thrombin-occupied	57:73	arg1	particles					94:102	thrombin-occupied microporous starch particles	57:102	thrombin-occupied microporous starch particles	57:102	Puff pastry-like chitosan/konjac glucomannan matrix with thrombin-occupied microporous starch particles as a composite for hemostasis.
31952612	3	25	theme	distributed	487:497	arg1	particles					518:526	evenly distributed microporous starch particles	480:526	evenly distributed microporous starch particles	480:526	The composite showed a hierarchical porous structure system consisting of porous and rough structures with evenly distributed microporous starch particles.
31952612	9	26	theme	hemostasis	1279:1288	arg1	control					1290:1296	hemostasis control	1279:1296	hemostasis control	1279:1296	Together, our results show that the puff pastry-like hemostat with hierarchical porous structure offers significant potential for use in hemostasis control.
31952612	9	27	theme	pastry-like	1183:1193	arg1	hemostat					1195:1202	the puff pastry-like hemostat	1174:1202	the puff pastry-like hemostat with hierarchical porous structure	1174:1237	Together, our results show that the puff pastry-like hemostat with hierarchical porous structure offers significant potential for use in hemostasis control.
31952612	3	28	with	structures	464:473	arg1	particles					518:526	evenly distributed microporous starch particles	480:526	evenly distributed microporous starch particles	480:526	The composite showed a hierarchical porous structure system consisting of porous and rough structures with evenly distributed microporous starch particles.
31952612	9	29	with	hemostat	1195:1202	arg1	structure					1229:1237	hierarchical porous structure	1209:1237	hierarchical porous structure	1209:1237	Together, our results show that the puff pastry-like hemostat with hierarchical porous structure offers significant potential for use in hemostasis control.
31952612	9	30	theme	puff	1178:1181	arg1	hemostat					1195:1202	the puff pastry-like hemostat	1174:1202	the puff pastry-like hemostat with hierarchical porous structure	1174:1237	Together, our results show that the puff pastry-like hemostat with hierarchical porous structure offers significant potential for use in hemostasis control.
31952612	6	31	theme	clotting	925:932	arg1	BCI					943:945	BCI	943:945	BCI	943:945	The composite was found to elicit desirable pro-thrombin time (PT), activated partial thrombin time (APTT), and whole blood clotting indices (BCI).
31952612	6	31	theme	clotting	925:932	arg1	indices					934:940	whole blood clotting indices	913:940	whole blood clotting indices (BCI)	913:946	The composite was found to elicit desirable pro-thrombin time (PT), activated partial thrombin time (APTT), and whole blood clotting indices (BCI).
31952612	2	32	theme	pastry-like	243:253	arg1	matrix					283:288	a puff pastry-like chitosan/konjac glucomannan matrix	236:288	a puff pastry-like chitosan/konjac glucomannan matrix loaded with thrombin-occupied microporous starch particles to initiate hemostasis	236:370	In this study, we produced a puff pastry-like chitosan/konjac glucomannan matrix loaded with thrombin-occupied microporous starch particles to initiate hemostasis.
31952612	3	33	theme	microporous	499:509	arg1	particles					518:526	evenly distributed microporous starch particles	480:526	evenly distributed microporous starch particles	480:526	The composite showed a hierarchical porous structure system consisting of porous and rough structures with evenly distributed microporous starch particles.
31952612	6	34	theme	blood	919:923	arg1	BCI					943:945	BCI	943:945	BCI	943:945	The composite was found to elicit desirable pro-thrombin time (PT), activated partial thrombin time (APTT), and whole blood clotting indices (BCI).
31952612	6	34	theme	blood	919:923	arg1	indices					934:940	whole blood clotting indices	913:940	whole blood clotting indices (BCI)	913:946	The composite was found to elicit desirable pro-thrombin time (PT), activated partial thrombin time (APTT), and whole blood clotting indices (BCI).
31952612	2	35	theme	puff	238:241	arg1	matrix					283:288	a puff pastry-like chitosan/konjac glucomannan matrix	236:288	a puff pastry-like chitosan/konjac glucomannan matrix loaded with thrombin-occupied microporous starch particles to initiate hemostasis	236:370	In this study, we produced a puff pastry-like chitosan/konjac glucomannan matrix loaded with thrombin-occupied microporous starch particles to initiate hemostasis.
31952612	9	36	theme	hierarchical	1209:1220	arg1	structure					1229:1237	hierarchical porous structure	1209:1237	hierarchical porous structure	1209:1237	Together, our results show that the puff pastry-like hemostat with hierarchical porous structure offers significant potential for use in hemostasis control.
31952612	0	37	theme	microporous	75:85	arg1	particles					94:102	thrombin-occupied microporous starch particles	57:102	thrombin-occupied microporous starch particles	57:102	Puff pastry-like chitosan/konjac glucomannan matrix with thrombin-occupied microporous starch particles as a composite for hemostasis.
31952612	6	38	theme	whole	913:917	arg1	BCI					943:945	BCI	943:945	BCI	943:945	The composite was found to elicit desirable pro-thrombin time (PT), activated partial thrombin time (APTT), and whole blood clotting indices (BCI).
31952612	6	38	theme	whole	913:917	arg1	indices					934:940	whole blood clotting indices	913:940	whole blood clotting indices (BCI)	913:946	The composite was found to elicit desirable pro-thrombin time (PT), activated partial thrombin time (APTT), and whole blood clotting indices (BCI).
31952612	9	39	theme	porous	1222:1227	arg1	structure					1229:1237	hierarchical porous structure	1209:1237	hierarchical porous structure	1209:1237	Together, our results show that the puff pastry-like hemostat with hierarchical porous structure offers significant potential for use in hemostasis control.
31952612	1	40	theme	Hemorrhage	135:144	arg1	control					146:152	Hemorrhage control	135:152	Hemorrhage control	135:152	Hemorrhage control is key for reducing mortality following severe trauma.
31952612	3	41	theme	rough	458:462	arg1	structures					464:473	porous and rough structures	447:473	porous and rough structures with evenly distributed microporous starch particles	447:526	The composite showed a hierarchical porous structure system consisting of porous and rough structures with evenly distributed microporous starch particles.
31952612	4	42	theme	hierarchical	626:637	arg1	system					639:644	the hierarchical system	622:644	the hierarchical system	622:644	Thrombin was evenly and independently distributed on the microporous starch particles within the hierarchical system and served to accelerate hemostasis.
31952612	6	43	theme	pro-thrombin	845:856	arg1	PT					864:865	PT	864:865	PT	864:865	The composite was found to elicit desirable pro-thrombin time (PT), activated partial thrombin time (APTT), and whole blood clotting indices (BCI).
31952612	6	43	theme	pro-thrombin	845:856	arg1	time					858:861	desirable pro-thrombin time	835:861	desirable pro-thrombin time (PT)	835:866	The composite was found to elicit desirable pro-thrombin time (PT), activated partial thrombin time (APTT), and whole blood clotting indices (BCI).
31952612	7	44	theme	animal	967:972	arg1	models					974:979	animal models	967:979	animal models	967:979	Experiments using animal models demonstrated that the composite could effectively control wound hemorrhage.
31952612	6	45	theme	desirable	835:843	arg1	PT					864:865	PT	864:865	PT	864:865	The composite was found to elicit desirable pro-thrombin time (PT), activated partial thrombin time (APTT), and whole blood clotting indices (BCI).
31952612	6	45	theme	desirable	835:843	arg1	time					858:861	desirable pro-thrombin time	835:861	desirable pro-thrombin time (PT)	835:866	The composite was found to elicit desirable pro-thrombin time (PT), activated partial thrombin time (APTT), and whole blood clotting indices (BCI).
31952612	9	46	theme	significant	1246:1256	arg1	potential					1258:1266	significant potential	1246:1266	significant potential for use in hemostasis control	1246:1296	Together, our results show that the puff pastry-like hemostat with hierarchical porous structure offers significant potential for use in hemostasis control.
31952612	2	47	theme	glucomannan	271:281	arg1	matrix					283:288	a puff pastry-like chitosan/konjac glucomannan matrix	236:288	a puff pastry-like chitosan/konjac glucomannan matrix loaded with thrombin-occupied microporous starch particles to initiate hemostasis	236:370	In this study, we produced a puff pastry-like chitosan/konjac glucomannan matrix loaded with thrombin-occupied microporous starch particles to initiate hemostasis.
31952612	9	48	from	use	1272:1274	arg1	control					1290:1296	hemostasis control	1279:1296	hemostasis control	1279:1296	Together, our results show that the puff pastry-like hemostat with hierarchical porous structure offers significant potential for use in hemostasis control.
31952612	0	49	theme	starch	87:92	arg1	particles					94:102	thrombin-occupied microporous starch particles	57:102	thrombin-occupied microporous starch particles	57:102	Puff pastry-like chitosan/konjac glucomannan matrix with thrombin-occupied microporous starch particles as a composite for hemostasis.
31952612	2	50	theme	chitosan/konjac	255:269	arg1	matrix					283:288	a puff pastry-like chitosan/konjac glucomannan matrix	236:288	a puff pastry-like chitosan/konjac glucomannan matrix loaded with thrombin-occupied microporous starch particles to initiate hemostasis	236:370	In this study, we produced a puff pastry-like chitosan/konjac glucomannan matrix loaded with thrombin-occupied microporous starch particles to initiate hemostasis.
33968068	9	0	theme	cancer	2194:2199	arg1	patients					2201:2208	cancer patients	2194:2208	cancer patients	2194:2208	These results suggest AG, particularly co-treated of polysaccharide and ginsenoside may be used as immunostimulants targeting microbiome-metabolomics axis to prevent CTX-induced side effects in cancer patients.
33968068	5	1	theme	mucins	1203:1208	arg1	expression					1210:1219	mucins expression	1203:1219	mucins expression	1203:1219	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	0	2	theme	Microbiome-Metabolomics	211:233	arg1	Analysis					235:242	Microbiome-Metabolomics Analysis	211:242	Microbiome-Metabolomics Analysis	211:242	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis.
33968068	8	3	theme	various	1776:1782	arg1	acid					1815:1818	uric acid	1810:1818	uric acid	1810:1818	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	8	3	theme	various	1776:1782	arg1	metabolites					1790:1800	various fecal metabolites	1776:1800	various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier	1776:1997	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	8	3	theme	various	1776:1782	arg1	so					1918:1919	so	1918:1919	so	1918:1919	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	1	4	theme	high	300:303	arg1	dose					305:308	high dose	300:308	high dose of which would cause immunosuppressive effect and intestinal mucosa damage	300:383	Cyclophosphamide (CTX), used in cancer chemotherapy, a high dose of which would cause immunosuppressive effect and intestinal mucosa damage.
33968068	4	5	theme	immune	863:868	arg1	disorder					870:877	the immune disorder	859:877	the immune disorder	859:877	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	4	6	theme	American	678:685	arg1	[AGP					728:731	American ginseng polysaccharide [AGP	696:731	American ginseng polysaccharide [AGP	696:731	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	4	6	theme	American	678:685	arg1	ginseng					687:693	American ginseng	678:693	American ginseng (American ginseng polysaccharide [AGP]	678:732	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	9	7	theme	co-treated	2039:2048	arg1	AG					2022:2023	AG	2022:2023	AG	2022:2023	These results suggest AG, particularly co-treated of polysaccharide and ginsenoside may be used as immunostimulants targeting microbiome-metabolomics axis to prevent CTX-induced side effects in cancer patients.
33968068	3	8	theme	American	585:592	arg1	effect					621:626	American ginseng's immunomodulatory effect	585:626	American ginseng's immunomodulatory effect	585:626	This study aimed to illustrate the underlying mechanism of American ginseng's immunomodulatory effect in CTX-induced mice.
33968068	0	9	theme	Cyclophosphamide-Induced	120:143	arg1	Disorders					163:171	Cyclophosphamide-Induced Intestinal Immune Disorders	120:171	Cyclophosphamide-Induced Intestinal Immune Disorders	120:171	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis.
33968068	7	10	dep	taxa	1616:1619	arg1	Lachnospiraceae					1657:1671	Lachnospiraceae	1657:1671	Lachnospiraceae	1657:1671	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	10	dep	taxa	1616:1619	arg1	Clostridiales					1621:1633	Clostridiales	1621:1633	Clostridiales	1621:1633	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	10	dep	taxa	1616:1619	arg1	taxa					1616:1619	increased various beneficial mucosa-associated bacterial taxa	1559:1619	increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae	1559:1671	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	10	dep	taxa	1616:1619	arg1	Bifidobacterium					1636:1650	Bifidobacterium	1636:1650	Bifidobacterium	1636:1650	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	0	11	theme	Immune	156:161	arg1	Disorders					163:171	Cyclophosphamide-Induced Intestinal Immune Disorders	120:171	Cyclophosphamide-Induced Intestinal Immune Disorders	120:171	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis.
33968068	2	12	contain	has	428:430	arg2	history					439:445	a long history	432:445	a long history of functional food use for immunological disorder, colitis, cancer, and so	432:520	American ginseng (Panax quinquefolius L.) has a long history of functional food use for immunological disorder, colitis, cancer, and so on.
33968068	2	12	contain	has	428:430	arg1	L.					424:425	Panax quinquefolius L.	404:425	Panax quinquefolius L.	404:425	American ginseng (Panax quinquefolius L.) has a long history of functional food use for immunological disorder, colitis, cancer, and so on.
33968068	2	12	contain	has	428:430	arg1	ginseng					395:401	American ginseng	386:401	American ginseng (Panax quinquefolius L.)	386:426	American ginseng (Panax quinquefolius L.) has a long history of functional food use for immunological disorder, colitis, cancer, and so on.
33968068	8	13	theme	xanthurenic	1821:1831	arg1	acid					1833:1836	xanthurenic acid	1821:1836	xanthurenic acid	1821:1836	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	8	13	theme	xanthurenic	1821:1831	arg1	acid					1815:1818	uric acid	1810:1818	uric acid	1810:1818	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	4	14	theme	subsets	907:913	arg1	ratio					915:919	the lymphocyte subsets ratio	892:919	the lymphocyte subsets ratio in spleen and peripheral blood	892:950	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	3	15	from	mechanism	572:580	arg1	mice					643:646	CTX-induced mice	631:646	CTX-induced mice	631:646	This study aimed to illustrate the underlying mechanism of American ginseng's immunomodulatory effect in CTX-induced mice.
33968068	0	16	theme	Barrier	181:187	arg1	Dysfunctions					189:200	Gut Barrier Dysfunctions	177:200	Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis	177:242	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis.
33968068	5	17	theme	AGP_AGG	1073:1079	arg1	group					1092:1096	AGP_AGG co-treated group	1073:1096	AGP_AGG co-treated group	1073:1096	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	5	17	theme	AGP_AGG	1073:1079	arg1	groups					1054:1059	These three treatment groups	1032:1059	These three treatment groups	1032:1059	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	4	18	theme	CD4+T	976:980	arg1	cells					1006:1010	CD4+T cells and IgA-secreting cells	976:1010	CD4+T cells and IgA-secreting cells in small intestine	976:1029	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	5	19	theme	junction	1272:1279	arg1	proteins					1281:1288	tight junction proteins	1266:1288	tight junction proteins (ZO-1, occludin)	1266:1305	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	0	20	theme	American	65:72	arg1	L.					103:104	Panax quinquefolius L.	83:104	Panax quinquefolius L.	83:104	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis.
33968068	0	20	theme	American	65:72	arg1	Ginseng					74:80	American Ginseng	65:80	American Ginseng (Panax quinquefolius L.)	65:105	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis.
33968068	5	21	theme	height	1158:1163	arg1	ratio					1187:1191	villus height (VH)/crypt depth (CD) ratio	1151:1191	villus height (VH)/crypt depth (CD) ratio	1151:1191	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	3	22	theme	CTX-induced	631:641	arg1	mice					643:646	CTX-induced mice	631:646	CTX-induced mice	631:646	This study aimed to illustrate the underlying mechanism of American ginseng's immunomodulatory effect in CTX-induced mice.
33968068	0	23	from	Effects	16:22	arg1	L.					103:104	Panax quinquefolius L.	83:104	Panax quinquefolius L.	83:104	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis.
33968068	0	23	from	Effects	16:22	arg1	Ginseng					74:80	American Ginseng	65:80	American Ginseng (Panax quinquefolius L.)	65:105	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis.
33968068	8	24	theme	gut	1987:1989	arg1	barrier					1991:1997	gut barrier	1987:1997	gut barrier	1987:1997	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	4	25	theme	ginseng	705:711	arg1	[AGP					728:731	American ginseng polysaccharide [AGP	696:731	American ginseng polysaccharide [AGP	696:731	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	4	25	theme	ginseng	705:711	arg1	ginseng					687:693	American ginseng	678:693	American ginseng (American ginseng polysaccharide [AGP]	678:732	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	7	26	theme	bacterial	1606:1614	arg1	Lachnospiraceae					1657:1671	Lachnospiraceae	1657:1671	Lachnospiraceae	1657:1671	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	26	theme	bacterial	1606:1614	arg1	Clostridiales					1621:1633	Clostridiales	1621:1633	Clostridiales	1621:1633	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	26	theme	bacterial	1606:1614	arg1	taxa					1616:1619	increased various beneficial mucosa-associated bacterial taxa	1559:1619	increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae	1559:1671	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	26	theme	bacterial	1606:1614	arg1	Bifidobacterium					1636:1650	Bifidobacterium	1636:1650	Bifidobacterium	1636:1650	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	9	27	theme	polysaccharide	2053:2066	arg1	co-treated					2039:2048	co-treated	2039:2048	co-treated	2039:2048	These results suggest AG, particularly co-treated of polysaccharide and ginsenoside may be used as immunostimulants targeting microbiome-metabolomics axis to prevent CTX-induced side effects in cancer patients.
33968068	0	28	theme	quinquefolius	89:101	arg1	L.					103:104	Panax quinquefolius L.	83:104	Panax quinquefolius L.	83:104	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis.
33968068	0	28	theme	quinquefolius	89:101	arg1	Ginseng					74:80	American Ginseng	65:80	American Ginseng (Panax quinquefolius L.)	65:105	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis.
33968068	7	29	theme	beneficial	1577:1586	arg1	Lachnospiraceae					1657:1671	Lachnospiraceae	1657:1671	Lachnospiraceae	1657:1671	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	29	theme	beneficial	1577:1586	arg1	Clostridiales					1621:1633	Clostridiales	1621:1633	Clostridiales	1621:1633	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	29	theme	beneficial	1577:1586	arg1	taxa					1616:1619	increased various beneficial mucosa-associated bacterial taxa	1559:1619	increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae	1559:1671	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	29	theme	beneficial	1577:1586	arg1	Bifidobacterium					1636:1650	Bifidobacterium	1636:1650	Bifidobacterium	1636:1650	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	9	30	theme	ginsenoside	2072:2082	arg1	co-treated					2039:2048	co-treated	2039:2048	co-treated	2039:2048	These results suggest AG, particularly co-treated of polysaccharide and ginsenoside may be used as immunostimulants targeting microbiome-metabolomics axis to prevent CTX-induced side effects in cancer patients.
33968068	6	31	theme	microbiome-metabolomics	1325:1347	arg1	analysis					1349:1356	the microbiome-metabolomics analysis	1321:1356	the microbiome-metabolomics analysis	1321:1356	Importantly, the microbiome-metabolomics analysis was applied in this study to illustrate the possible immuno-modulating mechanism.
33968068	2	32	theme	immunological	474:486	arg1	disorder					488:495	immunological disorder	474:495	immunological disorder	474:495	American ginseng (Panax quinquefolius L.) has a long history of functional food use for immunological disorder, colitis, cancer, and so on.
33968068	2	33	theme	so	519:520	arg1	history					439:445	a long history	432:445	a long history of functional food use for immunological disorder, colitis, cancer, and so	432:520	American ginseng (Panax quinquefolius L.) has a long history of functional food use for immunological disorder, colitis, cancer, and so on.
33968068	8	34	theme	LysoPI	1901:1906	arg1	18:0					1908:1911	LysoPI 18:0	1901:1911	LysoPI 18:0	1901:1911	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	8	34	theme	LysoPI	1901:1906	arg1	acid					1815:1818	uric acid	1810:1818	uric acid	1810:1818	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	2	35	theme	use	466:468	arg1	history					439:445	a long history	432:445	a long history of functional food use for immunological disorder, colitis, cancer, and so	432:520	American ginseng (Panax quinquefolius L.) has a long history of functional food use for immunological disorder, colitis, cancer, and so on.
33968068	2	36	theme	functional	450:459	arg1	use					466:468	functional food use	450:468	functional food use for immunological disorder	450:495	American ginseng (Panax quinquefolius L.) has a long history of functional food use for immunological disorder, colitis, cancer, and so on.
33968068	4	37	theme	small	1015:1019	arg1	intestine					1021:1029	small intestine	1015:1029	small intestine	1015:1029	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	6	38	theme	possible	1402:1409	arg1	mechanism					1429:1437	the possible immuno-modulating mechanism	1398:1437	the possible immuno-modulating mechanism	1398:1437	Importantly, the microbiome-metabolomics analysis was applied in this study to illustrate the possible immuno-modulating mechanism.
33968068	5	39	theme	/crypt	1169:1174	arg1	ratio					1187:1191	villus height (VH)/crypt depth (CD) ratio	1151:1191	villus height (VH)/crypt depth (CD) ratio	1151:1191	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	0	40	theme	Polysaccharides	27:41	arg1	Effects					16:22	The Synergistic Effects	0:22	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.)	0:105	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis.
33968068	7	41	theme	microbiota	1532:1541	arg1	composition					1543:1553	the gut microbiota composition	1524:1553	the gut microbiota composition	1524:1553	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	2	42	theme	American	386:393	arg1	L.					424:425	Panax quinquefolius L.	404:425	Panax quinquefolius L.	404:425	American ginseng (Panax quinquefolius L.) has a long history of functional food use for immunological disorder, colitis, cancer, and so on.
33968068	2	42	theme	American	386:393	arg1	ginseng					395:401	American ginseng	386:401	American ginseng (Panax quinquefolius L.)	386:426	American ginseng (Panax quinquefolius L.) has a long history of functional food use for immunological disorder, colitis, cancer, and so on.
33968068	0	43	theme	Ginsenosides	47:58	arg1	Effects					16:22	The Synergistic Effects	0:22	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.)	0:105	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis.
33968068	1	44	theme	mucosa	371:376	arg1	damage					378:383	intestinal mucosa damage	360:383	intestinal mucosa damage	360:383	Cyclophosphamide (CTX), used in cancer chemotherapy, a high dose of which would cause immunosuppressive effect and intestinal mucosa damage.
33968068	4	45	theme	ginseng	796:802	arg1	polysaccharide					804:817	co-treated with American ginseng polysaccharide	771:817	co-treated with American ginseng polysaccharide	771:817	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	4	46	from	cells	1006:1010	arg1	intestine					1021:1029	small intestine	1015:1029	small intestine	1015:1029	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	9	47	theme	side	2178:2181	arg1	effects					2183:2189	CTX-induced side effects	2166:2189	CTX-induced side effects	2166:2189	These results suggest AG, particularly co-treated of polysaccharide and ginsenoside may be used as immunostimulants targeting microbiome-metabolomics axis to prevent CTX-induced side effects in cancer patients.
33968068	2	48	theme	quinquefolius	410:422	arg1	L.					424:425	Panax quinquefolius L.	404:425	Panax quinquefolius L.	404:425	American ginseng (Panax quinquefolius L.) has a long history of functional food use for immunological disorder, colitis, cancer, and so on.
33968068	2	48	theme	quinquefolius	410:422	arg1	ginseng					395:401	American ginseng	386:401	American ginseng (Panax quinquefolius L.)	386:426	American ginseng (Panax quinquefolius L.) has a long history of functional food use for immunological disorder, colitis, cancer, and so on.
33968068	5	49	theme	goblet	1234:1239	arg1	cells					1241:1245	goblet cells	1234:1245	goblet cells	1234:1245	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	7	50	theme	AGP_AGG	1500:1506	arg1	polysaccharides					1466:1480	polysaccharides	1466:1480	polysaccharides	1466:1480	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	50	theme	AGP_AGG	1500:1506	arg1	group					1508:1512	AGP_AGG group	1500:1512	AGP_AGG group	1500:1512	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	51	theme	ginsenosides	1486:1497	arg1	effect					1456:1461	The synergistic effect	1440:1461	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group)	1440:1513	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	5	52	dep	proteins	1281:1288	arg1	occludin					1297:1304	occludin	1297:1304	occludin	1297:1304	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	5	52	dep	proteins	1281:1288	arg1	ZO-1					1291:1294	ZO-1	1291:1294	ZO-1	1291:1294	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	8	53	theme	AGP_AGG	1754:1760	arg1	group					1762:1766	AGP_AGG group	1754:1766	AGP_AGG group	1754:1766	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	5	54	theme	expression	1210:1219	arg1	expression					1252:1261	expression	1252:1261	expression of tight junction proteins (ZO-1, occludin)	1252:1305	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	5	54	theme	expression	1210:1219	arg1	morphology					1122:1131	the intestine morphology	1108:1131	the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio	1108:1191	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	5	54	theme	expression	1210:1219	arg1	quantity					1222:1229	quantity	1222:1229	quantity of goblet cells	1222:1245	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	5	54	theme	expression	1210:1219	arg1	areas					1194:1198	areas	1194:1198	areas of mucins expression	1194:1219	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	7	55	theme	polysaccharides	1466:1480	arg1	effect					1456:1461	The synergistic effect	1440:1461	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group)	1440:1513	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	56	theme	synergistic	1444:1454	arg1	effect					1456:1461	The synergistic effect	1440:1461	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group)	1440:1513	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	9	57	dep	suggest	2014:2020	arg1	used					2091:2094	used	2091:2094	suggest AG, particularly co-treated of polysaccharide and ginsenoside may be used as immunostimulants targeting microbiome-metabolomics axis to prevent CTX-induced side effects in cancer patients	2014:2208	These results suggest AG, particularly co-treated of polysaccharide and ginsenoside may be used as immunostimulants targeting microbiome-metabolomics axis to prevent CTX-induced side effects in cancer patients.
33968068	4	58	theme	cells and IgA-secreting	982:1004	arg1	cells					1006:1010	CD4+T cells and IgA-secreting cells	976:1010	CD4+T cells and IgA-secreting cells in small intestine	976:1029	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	8	59	theme	fecal	1784:1788	arg1	acid					1815:1818	uric acid	1810:1818	uric acid	1810:1818	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	8	59	theme	fecal	1784:1788	arg1	metabolites					1790:1800	various fecal metabolites	1776:1800	various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier	1776:1997	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	8	59	theme	fecal	1784:1788	arg1	so					1918:1919	so	1918:1919	so	1918:1919	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	7	60	theme	harmful	1690:1696	arg1	Peptococcaceae					1732:1745	Peptococcaceae	1732:1745	Peptococcaceae	1732:1745	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	60	theme	harmful	1690:1696	arg1	Escherichia-Shigella					1707:1726	Escherichia-Shigella	1707:1726	Escherichia-Shigella	1707:1726	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	60	theme	harmful	1690:1696	arg1	bacteria					1698:1705	harmful bacteria	1690:1705	harmful bacteria Escherichia-Shigella and Peptococcaceae	1690:1745	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	4	61	with	co-treated	771:780	arg1	American					787:794	American	787:794	American	787:794	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	0	62	theme	Intestinal	145:154	arg1	Disorders					163:171	Cyclophosphamide-Induced Intestinal Immune Disorders	120:171	Cyclophosphamide-Induced Intestinal Immune Disorders	120:171	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis.
33968068	3	63	theme	underlying	561:570	arg1	mechanism					572:580	the underlying mechanism	557:580	the underlying mechanism of American ginseng's immunomodulatory effect in CTX-induced mice	557:646	This study aimed to illustrate the underlying mechanism of American ginseng's immunomodulatory effect in CTX-induced mice.
33968068	8	64	theme	protective	1966:1975	arg1	effect					1977:1982	protective effect	1966:1982	protective effect of gut barrier	1966:1997	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	3	65	theme	immunomodulatory	604:619	arg1	effect					621:626	American ginseng's immunomodulatory effect	585:626	American ginseng's immunomodulatory effect	585:626	This study aimed to illustrate the underlying mechanism of American ginseng's immunomodulatory effect in CTX-induced mice.
33968068	8	66	theme	uric	1810:1813	arg1	LysoPE15:0					1876:1885	LysoPE15:0	1876:1885	LysoPE15:0	1876:1885	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	8	66	theme	uric	1810:1813	arg1	18:0					1908:1911	LysoPI 18:0	1901:1911	LysoPI 18:0	1901:1911	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	8	66	theme	uric	1810:1813	arg1	acylcarnitine					1839:1851	acylcarnitine	1839:1851	acylcarnitine	1839:1851	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	8	66	theme	uric	1810:1813	arg1	acid					1815:1818	uric acid	1810:1818	uric acid	1810:1818	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	8	66	theme	uric	1810:1813	arg1	9,10-DHOME					1854:1863	9,10-DHOME	1854:1863	9,10-DHOME	1854:1863	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	8	66	theme	uric	1810:1813	arg1	16:0					1895:1898	LysoPC 16:0	1888:1898	LysoPC 16:0	1888:1898	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	8	66	theme	uric	1810:1813	arg1	acid					1833:1836	xanthurenic acid	1821:1836	xanthurenic acid	1821:1836	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	8	66	theme	uric	1810:1813	arg1	13-HDoHE					1866:1873	13-HDoHE	1866:1873	13-HDoHE	1866:1873	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	4	67	theme	peripheral	935:944	arg1	blood					946:950	peripheral blood	935:950	peripheral blood	935:950	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	0	68	from	Ginseng	74:80	arg1	Effects					16:22	The Synergistic Effects	0:22	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.)	0:105	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis.
33968068	0	68	from	Ginseng	74:80	arg1	Ginsenosides					47:58	Ginsenosides	47:58	Ginsenosides	47:58	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis.
33968068	0	68	from	Ginseng	74:80	arg1	Polysaccharides					27:41	Polysaccharides	27:41	Polysaccharides	27:41	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis.
33968068	1	69	theme	intestinal	360:369	arg1	damage					378:383	intestinal mucosa damage	360:383	intestinal mucosa damage	360:383	Cyclophosphamide (CTX), used in cancer chemotherapy, a high dose of which would cause immunosuppressive effect and intestinal mucosa damage.
33968068	0	70	theme	Gut	177:179	arg1	Dysfunctions					189:200	Gut Barrier Dysfunctions	177:200	Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis	177:242	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis.
33968068	5	71	theme	intestine	1112:1120	arg1	morphology					1122:1131	the intestine morphology	1108:1131	the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio	1108:1191	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	4	72	theme	lymphocyte	896:905	arg1	subsets					907:913	lymphocyte subsets	896:913	the lymphocyte subsets ratio in spleen and peripheral blood	892:950	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	4	73	theme	polysaccharide	713:726	arg1	[AGP					728:731	American ginseng polysaccharide [AGP	696:731	American ginseng polysaccharide [AGP	696:731	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	4	73	theme	polysaccharide	713:726	arg1	ginseng					687:693	American ginseng	678:693	American ginseng (American ginseng polysaccharide [AGP]	678:732	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	5	74	theme	tight	1266:1270	arg1	proteins					1281:1288	tight junction proteins	1266:1288	tight junction proteins (ZO-1, occludin)	1266:1305	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	8	75	theme	barrier	1991:1997	arg1	immunometabolism					1946:1961	immunometabolism	1946:1961	immunometabolism	1946:1961	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	8	75	theme	barrier	1991:1997	arg1	effect					1977:1982	protective effect	1966:1982	protective effect of gut barrier	1966:1997	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	4	76	from	ratio	915:919	arg1	spleen					924:929	spleen	924:929	spleen	924:929	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	4	76	from	ratio	915:919	arg1	blood					946:950	peripheral blood	935:950	peripheral blood	935:950	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	5	77	theme	proteins	1281:1288	arg1	expression					1252:1261	expression	1252:1261	expression of tight junction proteins (ZO-1, occludin)	1252:1305	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	5	77	theme	proteins	1281:1288	arg1	morphology					1122:1131	the intestine morphology	1108:1131	the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio	1108:1191	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	5	77	theme	proteins	1281:1288	arg1	quantity					1222:1229	quantity	1222:1229	quantity of goblet cells	1222:1245	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	5	77	theme	proteins	1281:1288	arg1	areas					1194:1198	areas	1194:1198	areas of mucins expression	1194:1219	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	5	78	theme	co-treated	1081:1090	arg1	group					1092:1096	AGP_AGG co-treated group	1073:1096	AGP_AGG co-treated group	1073:1096	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	5	78	theme	co-treated	1081:1090	arg1	groups					1054:1059	These three treatment groups	1032:1059	These three treatment groups	1032:1059	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	4	79	theme	ginseng	687:693	arg1	groups					668:673	all groups	664:673	all groups of American ginseng (American ginseng polysaccharide [AGP]	664:732	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	4	79	theme	ginseng	687:693	arg1	ginseng					687:693	American ginseng	678:693	American ginseng (American ginseng polysaccharide [AGP]	678:732	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	4	79	theme	ginseng	687:693	arg1	American					735:742	American	735:742	American	735:742	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	4	79	theme	ginseng	687:693	arg1	[AGP					728:731	American ginseng polysaccharide [AGP	696:731	American ginseng polysaccharide [AGP	696:731	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	0	80	theme	Panax	83:87	arg1	L.					103:104	Panax quinquefolius L.	83:104	Panax quinquefolius L.	83:104	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis.
33968068	0	80	theme	Panax	83:87	arg1	Ginseng					74:80	American Ginseng	65:80	American Ginseng (Panax quinquefolius L.)	65:105	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis.
33968068	7	81	theme	mucosa-associated	1588:1604	arg1	Lachnospiraceae					1657:1671	Lachnospiraceae	1657:1671	Lachnospiraceae	1657:1671	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	81	theme	mucosa-associated	1588:1604	arg1	Clostridiales					1621:1633	Clostridiales	1621:1633	Clostridiales	1621:1633	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	81	theme	mucosa-associated	1588:1604	arg1	taxa					1616:1619	increased various beneficial mucosa-associated bacterial taxa	1559:1619	increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae	1559:1671	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	81	theme	mucosa-associated	1588:1604	arg1	Bifidobacterium					1636:1650	Bifidobacterium	1636:1650	Bifidobacterium	1636:1650	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	2	82	theme	cancer	507:512	arg1	history					439:445	a long history	432:445	a long history of functional food use for immunological disorder, colitis, cancer, and so	432:520	American ginseng (Panax quinquefolius L.) has a long history of functional food use for immunological disorder, colitis, cancer, and so on.
33968068	4	83	theme	American	696:703	arg1	[AGP					728:731	American ginseng polysaccharide [AGP	696:731	American ginseng polysaccharide [AGP	696:731	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	4	83	theme	American	696:703	arg1	ginseng					687:693	American ginseng	678:693	American ginseng (American ginseng polysaccharide [AGP]	678:732	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	7	84	dep	bacteria	1698:1705	arg1	Peptococcaceae					1732:1745	Peptococcaceae	1732:1745	Peptococcaceae	1732:1745	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	84	dep	bacteria	1698:1705	arg1	Escherichia-Shigella					1707:1726	Escherichia-Shigella	1707:1726	Escherichia-Shigella	1707:1726	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	84	dep	bacteria	1698:1705	arg1	bacteria					1698:1705	harmful bacteria	1690:1705	harmful bacteria Escherichia-Shigella and Peptococcaceae	1690:1745	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	85	theme	various	1569:1575	arg1	Lachnospiraceae					1657:1671	Lachnospiraceae	1657:1671	Lachnospiraceae	1657:1671	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	85	theme	various	1569:1575	arg1	Clostridiales					1621:1633	Clostridiales	1621:1633	Clostridiales	1621:1633	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	85	theme	various	1569:1575	arg1	taxa					1616:1619	increased various beneficial mucosa-associated bacterial taxa	1559:1619	increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae	1559:1671	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	85	theme	various	1569:1575	arg1	Bifidobacterium					1636:1650	Bifidobacterium	1636:1650	Bifidobacterium	1636:1650	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	4	86	theme	co-treated	771:780	arg1	polysaccharide					804:817	co-treated with American ginseng polysaccharide	771:817	co-treated with American ginseng polysaccharide	771:817	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	0	87	theme	Synergistic	4:14	arg1	Effects					16:22	The Synergistic Effects	0:22	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.)	0:105	The Synergistic Effects of Polysaccharides and Ginsenosides From American Ginseng (Panax quinquefolius L.) Ameliorating Cyclophosphamide-Induced Intestinal Immune Disorders and Gut Barrier Dysfunctions Based on Microbiome-Metabolomics Analysis.
33968068	5	88	theme	villus	1151:1156	arg1	VH					1166:1167	VH	1166:1167	VH	1166:1167	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	5	88	theme	villus	1151:1156	arg1	height					1158:1163	villus height	1151:1163	villus height (VH)/crypt depth (CD) ratio	1151:1191	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	8	89	theme	LysoPC	1888:1893	arg1	16:0					1895:1898	LysoPC 16:0	1888:1898	LysoPC 16:0	1888:1898	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	8	89	theme	LysoPC	1888:1893	arg1	acid					1815:1818	uric acid	1810:1818	uric acid	1810:1818	Also, AGP_AGG group altered various fecal metabolites such as uric acid, xanthurenic acid, acylcarnitine, 9,10-DHOME, 13-HDoHE, LysoPE15:0, LysoPC 16:0, LysoPI 18:0, and so on, that associated with immunometabolism or protective effect of gut barrier.
33968068	2	90	theme	food	461:464	arg1	use					466:468	functional food use	450:468	functional food use for immunological disorder	450:495	American ginseng (Panax quinquefolius L.) has a long history of functional food use for immunological disorder, colitis, cancer, and so on.
33968068	4	91	theme	ginsenoside	752:762	arg1	[AGG					764:767	ginsenoside [AGG	752:767	ginsenoside [AGG	752:767	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	6	92	theme	immuno-modulating	1411:1427	arg1	mechanism					1429:1437	the possible immuno-modulating mechanism	1398:1437	the possible immuno-modulating mechanism	1398:1437	Importantly, the microbiome-metabolomics analysis was applied in this study to illustrate the possible immuno-modulating mechanism.
33968068	7	93	theme	increased	1559:1567	arg1	Lachnospiraceae					1657:1671	Lachnospiraceae	1657:1671	Lachnospiraceae	1657:1671	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	93	theme	increased	1559:1567	arg1	Clostridiales					1621:1633	Clostridiales	1621:1633	Clostridiales	1621:1633	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	93	theme	increased	1559:1567	arg1	taxa					1616:1619	increased various beneficial mucosa-associated bacterial taxa	1559:1619	increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae	1559:1671	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	7	93	theme	increased	1559:1567	arg1	Bifidobacterium					1636:1650	Bifidobacterium	1636:1650	Bifidobacterium	1636:1650	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	2	94	theme	colitis	498:504	arg1	history					439:445	a long history	432:445	a long history of functional food use for immunological disorder, colitis, cancer, and so	432:520	American ginseng (Panax quinquefolius L.) has a long history of functional food use for immunological disorder, colitis, cancer, and so on.
33968068	9	95	theme	microbiome-metabolomics	2126:2148	arg1	axis					2150:2153	microbiome-metabolomics axis	2126:2153	microbiome-metabolomics axis	2126:2153	These results suggest AG, particularly co-treated of polysaccharide and ginsenoside may be used as immunostimulants targeting microbiome-metabolomics axis to prevent CTX-induced side effects in cancer patients.
33968068	4	96	theme	ginsenoside	823:833	arg1	[AGP_AGG					835:842	ginsenoside [AGP_AGG	823:842	ginsenoside [AGP_AGG	823:842	In this study, all groups of American ginseng (American ginseng polysaccharide [AGP], American ginseng ginsenoside [AGG], co-treated with American ginseng polysaccharide and ginsenoside [AGP_AGG]) have relieve the immune disorder by reversing the lymphocyte subsets ratio in spleen and peripheral blood, as well as stimulating CD4+T cells and IgA-secreting cells in small intestine.
33968068	2	97	theme	long	434:437	arg1	history					439:445	a long history	432:445	a long history of functional food use for immunological disorder, colitis, cancer, and so	432:520	American ginseng (Panax quinquefolius L.) has a long history of functional food use for immunological disorder, colitis, cancer, and so on.
33968068	5	98	theme	depth	1176:1180	arg1	ratio					1187:1191	villus height (VH)/crypt depth (CD) ratio	1151:1191	villus height (VH)/crypt depth (CD) ratio	1151:1191	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	5	99	theme	treatment	1044:1052	arg1	group					1092:1096	AGP_AGG co-treated group	1073:1096	AGP_AGG co-treated group	1073:1096	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	5	99	theme	treatment	1044:1052	arg1	groups					1054:1059	These three treatment groups	1032:1059	These three treatment groups	1032:1059	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	5	100	theme	CD	1183:1184	arg1	ratio					1187:1191	villus height (VH)/crypt depth (CD) ratio	1151:1191	villus height (VH)/crypt depth (CD) ratio	1151:1191	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	7	101	theme	gut	1528:1530	arg1	composition					1543:1553	the gut microbiota composition	1524:1553	the gut microbiota composition	1524:1553	The synergistic effect of polysaccharides and ginsenosides (AGP_AGG group) restored the gut microbiota composition and increased various beneficial mucosa-associated bacterial taxa Clostridiales, Bifidobacterium, and Lachnospiraceae, while decreased harmful bacteria Escherichia-Shigella and Peptococcaceae.
33968068	9	102	theme	CTX-induced	2166:2176	arg1	effects					2183:2189	CTX-induced side effects	2166:2189	CTX-induced side effects	2166:2189	These results suggest AG, particularly co-treated of polysaccharide and ginsenoside may be used as immunostimulants targeting microbiome-metabolomics axis to prevent CTX-induced side effects in cancer patients.
33968068	1	103	theme	in cancer	274:282	arg1	chemotherapy					284:295	in cancer chemotherapy	274:295	in cancer chemotherapy	274:295	Cyclophosphamide (CTX), used in cancer chemotherapy, a high dose of which would cause immunosuppressive effect and intestinal mucosa damage.
33968068	2	104	theme	Panax	404:408	arg1	L.					424:425	Panax quinquefolius L.	404:425	Panax quinquefolius L.	404:425	American ginseng (Panax quinquefolius L.) has a long history of functional food use for immunological disorder, colitis, cancer, and so on.
33968068	2	104	theme	Panax	404:408	arg1	ginseng					395:401	American ginseng	386:401	American ginseng (Panax quinquefolius L.)	386:426	American ginseng (Panax quinquefolius L.) has a long history of functional food use for immunological disorder, colitis, cancer, and so on.
33968068	5	105	theme	cells	1241:1245	arg1	expression					1252:1261	expression	1252:1261	expression of tight junction proteins (ZO-1, occludin)	1252:1305	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	5	105	theme	cells	1241:1245	arg1	morphology					1122:1131	the intestine morphology	1108:1131	the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio	1108:1191	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	5	105	theme	cells	1241:1245	arg1	quantity					1222:1229	quantity	1222:1229	quantity of goblet cells	1222:1245	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
33968068	5	105	theme	cells	1241:1245	arg1	areas					1194:1198	areas	1194:1198	areas of mucins expression	1194:1219	These three treatment groups, especially AGP_AGG co-treated group recovered the intestine morphology that up-regulated villus height (VH)/crypt depth (CD) ratio, areas of mucins expression, quantity of goblet cells, and expression of tight junction proteins (ZO-1, occludin).
34827630	10	0	from	occurrence	1414:1423	arg1	organisms					1478:1486	evolutionarily very divergent organisms	1448:1486	evolutionarily very divergent organisms	1448:1486	The widespread occurrence of dCTRs in LPMOs from evolutionarily very divergent organisms, hints at a possible functional role and opens new prospects in the field of LPMOs.
34827630	10	0	from	occurrence	1414:1423	arg1	LPMOs					1437:1441	LPMOs	1437:1441	LPMOs from evolutionarily very divergent organisms	1437:1486	The widespread occurrence of dCTRs in LPMOs from evolutionarily very divergent organisms, hints at a possible functional role and opens new prospects in the field of LPMOs.
34827630	6	1	theme	composition	1049:1059	arg1	terms					1009:1013	terms	1009:1013	terms of sequence length and amino-acid composition	1009:1059	Our analysis shows that these disordered C-terminal regions (dCTRs) are widespread in all LPMO families (except AA13) and differ in terms of sequence length and amino-acid composition.
34827630	10	2	from	prospects	1539:1547	arg1	field					1556:1560	the field	1552:1560	the field of LPMOs	1552:1569	The widespread occurrence of dCTRs in LPMOs from evolutionarily very divergent organisms, hints at a possible functional role and opens new prospects in the field of LPMOs.
34827630	8	3	from	enrichment	1184:1193	arg1	threonine					1209:1217	threonine	1209:1217	threonine	1209:1217	LPMO-dCTRs share a common polyampholytic nature and an enrichment in serine and threonine residues, suggesting that they undergo post-translational modifications.
34827630	8	3	from	enrichment	1184:1193	arg1	serine					1198:1203	serine	1198:1203	serine	1198:1203	LPMO-dCTRs share a common polyampholytic nature and an enrichment in serine and threonine residues, suggesting that they undergo post-translational modifications.
34827630	10	4	theme	dCTRs	1428:1432	arg1	occurrence					1414:1423	The widespread occurrence	1399:1423	The widespread occurrence of dCTRs in LPMOs from evolutionarily very divergent organisms	1399:1486	The widespread occurrence of dCTRs in LPMOs from evolutionarily very divergent organisms, hints at a possible functional role and opens new prospects in the field of LPMOs.
34827630	1	5	theme	monocopper	197:206	arg1	monooxygenases					170:183	Lytic polysaccharide monooxygenases	149:183	Lytic polysaccharide monooxygenases (LPMOs)	149:191	Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes secreted by many organisms and viruses.
34827630	1	5	theme	monocopper	197:206	arg1	enzymes					208:214	monocopper enzymes	197:214	monocopper enzymes secreted by many organisms and viruses	197:253	Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes secreted by many organisms and viruses.
34827630	7	6	located	observed	1110:1117	arg2	dCTRs					1082:1086	these dCTRs	1076:1086	these dCTRs	1076:1086	Noteworthily, these dCTRs have so far only been observed in LPMOs.
34827630	7	6	located	observed	1110:1117	arg1	LPMOs					1122:1126	LPMOs	1122:1126	LPMOs	1122:1126	Noteworthily, these dCTRs have so far only been observed in LPMOs.
34827630	4	7	theme	LPMO	608:611	arg1	sequences					613:621	some LPMO sequences	603:621	some LPMO sequences	603:621	Intriguingly, we observed that some LPMO sequences also display a C-terminal extension of varying length not associated with any known function or fold.
34827630	0	8	theme	C-Terminal	126:135	arg1	Extensions					137:146	Intrinsically Disordered C-Terminal Extensions	101:146	Intrinsically Disordered C-Terminal Extensions	101:146	Bioinformatic Analysis of Lytic Polysaccharide Monooxygenases Reveals the Pan-Families Occurrence of Intrinsically Disordered C-Terminal Extensions.
34827630	9	9	dep	redox-sensitive	1348:1362	arg1	disordered					1379:1388	disordered	1379:1388	disordered	1379:1388	Interestingly, dCTRs from AA11 and AA15 are enriched in redox-sensitive, conditionally disordered regions.
34827630	10	10	theme	divergent	1468:1476	arg1	organisms					1478:1486	evolutionarily very divergent organisms	1448:1486	evolutionarily very divergent organisms	1448:1486	The widespread occurrence of dCTRs in LPMOs from evolutionarily very divergent organisms, hints at a possible functional role and opens new prospects in the field of LPMOs.
34827630	0	11	theme	Disordered	115:124	arg1	Extensions					137:146	Intrinsically Disordered C-Terminal Extensions	101:146	Intrinsically Disordered C-Terminal Extensions	101:146	Bioinformatic Analysis of Lytic Polysaccharide Monooxygenases Reveals the Pan-Families Occurrence of Intrinsically Disordered C-Terminal Extensions.
34827630	5	12	theme	different	765:773	arg1	families					780:787	different LPMO families	765:787	different LPMO families	765:787	Here, we analyzed 27,060 sequences from different LPMO families and show that 60% have a C-terminal extension predicted to be intrinsically disordered.
34827630	6	13	theme	C-terminal	918:927	arg1	dCTRs					938:942	dCTRs	938:942	dCTRs	938:942	Our analysis shows that these disordered C-terminal regions (dCTRs) are widespread in all LPMO families (except AA13) and differ in terms of sequence length and amino-acid composition.
34827630	6	13	theme	C-terminal	918:927	arg1	regions					929:935	C-terminal regions	918:935	these disordered C-terminal regions (dCTRs)	901:943	Our analysis shows that these disordered C-terminal regions (dCTRs) are widespread in all LPMO families (except AA13) and differ in terms of sequence length and amino-acid composition.
34827630	10	14	theme	possible	1500:1507	arg1	role					1520:1523	a possible functional role	1498:1523	a possible functional role	1498:1523	The widespread occurrence of dCTRs in LPMOs from evolutionarily very divergent organisms, hints at a possible functional role and opens new prospects in the field of LPMOs.
34827630	4	15	theme	varying	662:668	arg1	length					670:675	varying length	662:675	varying length	662:675	Intriguingly, we observed that some LPMO sequences also display a C-terminal extension of varying length not associated with any known function or fold.
34827630	2	16	theme	polysaccharides	316:330	arg1	types					307:311	different types	297:311	different types of polysaccharides	297:330	LPMOs catalyze the oxidative cleavage of different types of polysaccharides and are today divided into eight families (AA9-11, AA13-17) within the Auxiliary Activity enzyme class of the CAZy database.
34827630	3	17	theme	minimal	463:469	arg1	architecture					471:482	LPMOs minimal architecture	457:482	LPMOs minimal architecture	457:482	LPMOs minimal architecture encompasses a catalytic domain, to which can be appended a carbohydrate-binding module.
34827630	0	18	theme	Extensions	137:146	arg1	Occurrence					87:96	the Pan-Families Occurrence	70:96	the Pan-Families Occurrence of Intrinsically Disordered C-Terminal Extensions	70:146	Bioinformatic Analysis of Lytic Polysaccharide Monooxygenases Reveals the Pan-Families Occurrence of Intrinsically Disordered C-Terminal Extensions.
34827630	6	19	theme	disordered	907:916	arg1	dCTRs					938:942	dCTRs	938:942	dCTRs	938:942	Our analysis shows that these disordered C-terminal regions (dCTRs) are widespread in all LPMO families (except AA13) and differ in terms of sequence length and amino-acid composition.
34827630	6	19	theme	disordered	907:916	arg1	regions					929:935	C-terminal regions	918:935	these disordered C-terminal regions (dCTRs)	901:943	Our analysis shows that these disordered C-terminal regions (dCTRs) are widespread in all LPMO families (except AA13) and differ in terms of sequence length and amino-acid composition.
34827630	3	20	theme	LPMOs	457:461	arg1	architecture					471:482	LPMOs minimal architecture	457:482	LPMOs minimal architecture	457:482	LPMOs minimal architecture encompasses a catalytic domain, to which can be appended a carbohydrate-binding module.
34827630	10	21	theme	functional	1509:1518	arg1	role					1520:1523	a possible functional role	1498:1523	a possible functional role	1498:1523	The widespread occurrence of dCTRs in LPMOs from evolutionarily very divergent organisms, hints at a possible functional role and opens new prospects in the field of LPMOs.
34827630	2	22	theme	enzyme	422:427	arg1	class					429:433	the Auxiliary Activity enzyme class	399:433	the Auxiliary Activity enzyme class of the CAZy database	399:454	LPMOs catalyze the oxidative cleavage of different types of polysaccharides and are today divided into eight families (AA9-11, AA13-17) within the Auxiliary Activity enzyme class of the CAZy database.
34827630	5	23	theme	C-terminal	814:823	arg1	extension					825:833	a C-terminal extension	812:833	a C-terminal extension predicted to be intrinsically disordered	812:874	Here, we analyzed 27,060 sequences from different LPMO families and show that 60% have a C-terminal extension predicted to be intrinsically disordered.
34827630	6	24	theme	length	1027:1032	arg1	terms					1009:1013	terms	1009:1013	terms of sequence length and amino-acid composition	1009:1059	Our analysis shows that these disordered C-terminal regions (dCTRs) are widespread in all LPMO families (except AA13) and differ in terms of sequence length and amino-acid composition.
34827630	2	25	theme	Activity	413:420	arg1	class					429:433	the Auxiliary Activity enzyme class	399:433	the Auxiliary Activity enzyme class of the CAZy database	399:454	LPMOs catalyze the oxidative cleavage of different types of polysaccharides and are today divided into eight families (AA9-11, AA13-17) within the Auxiliary Activity enzyme class of the CAZy database.
34827630	0	26	theme	Bioinformatic	0:12	arg1	Analysis					14:21	Bioinformatic Analysis	0:21	Bioinformatic Analysis of Lytic Polysaccharide Monooxygenases	0:60	Bioinformatic Analysis of Lytic Polysaccharide Monooxygenases Reveals the Pan-Families Occurrence of Intrinsically Disordered C-Terminal Extensions.
34827630	2	27	dep	families	365:372	arg1	AA9-11					375:380	AA9-11	375:380	AA9-11	375:380	LPMOs catalyze the oxidative cleavage of different types of polysaccharides and are today divided into eight families (AA9-11, AA13-17) within the Auxiliary Activity enzyme class of the CAZy database.
34827630	2	27	dep	families	365:372	arg1	AA13-17					383:389	AA13-17	383:389	AA13-17	383:389	LPMOs catalyze the oxidative cleavage of different types of polysaccharides and are today divided into eight families (AA9-11, AA13-17) within the Auxiliary Activity enzyme class of the CAZy database.
34827630	8	28	theme	post-translational	1258:1275	arg1	modifications					1277:1289	post-translational modifications	1258:1289	post-translational modifications	1258:1289	LPMO-dCTRs share a common polyampholytic nature and an enrichment in serine and threonine residues, suggesting that they undergo post-translational modifications.
34827630	1	29	theme	many	228:231	arg1	organisms					233:241	many organisms	228:241	many organisms	228:241	Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes secreted by many organisms and viruses.
34827630	0	30	theme	Lytic	26:30	arg1	Monooxygenases					47:60	Lytic Polysaccharide Monooxygenases	26:60	Lytic Polysaccharide Monooxygenases	26:60	Bioinformatic Analysis of Lytic Polysaccharide Monooxygenases Reveals the Pan-Families Occurrence of Intrinsically Disordered C-Terminal Extensions.
34827630	9	31	theme	redox-sensitive	1348:1362	arg1	regions					1390:1396	redox-sensitive, conditionally disordered regions	1348:1396	redox-sensitive, conditionally disordered regions	1348:1396	Interestingly, dCTRs from AA11 and AA15 are enriched in redox-sensitive, conditionally disordered regions.
34827630	10	32	theme	widespread	1403:1412	arg1	occurrence					1414:1423	The widespread occurrence	1399:1423	The widespread occurrence of dCTRs in LPMOs from evolutionarily very divergent organisms	1399:1486	The widespread occurrence of dCTRs in LPMOs from evolutionarily very divergent organisms, hints at a possible functional role and opens new prospects in the field of LPMOs.
34827630	2	33	theme	database	447:454	arg1	class					429:433	the Auxiliary Activity enzyme class	399:433	the Auxiliary Activity enzyme class of the CAZy database	399:454	LPMOs catalyze the oxidative cleavage of different types of polysaccharides and are today divided into eight families (AA9-11, AA13-17) within the Auxiliary Activity enzyme class of the CAZy database.
34827630	9	34	from	AA15	1327:1330	arg1	dCTRs					1307:1311	dCTRs	1307:1311	dCTRs from AA11 and AA15	1307:1330	Interestingly, dCTRs from AA11 and AA15 are enriched in redox-sensitive, conditionally disordered regions.
34827630	6	35	from	families	972:979	arg1	widespread					949:958	widespread	949:958	widespread	949:958	Our analysis shows that these disordered C-terminal regions (dCTRs) are widespread in all LPMO families (except AA13) and differ in terms of sequence length and amino-acid composition.
34827630	2	36	theme	CAZy	442:445	arg1	database					447:454	the CAZy database	438:454	the CAZy database	438:454	LPMOs catalyze the oxidative cleavage of different types of polysaccharides and are today divided into eight families (AA9-11, AA13-17) within the Auxiliary Activity enzyme class of the CAZy database.
34827630	0	37	theme	Monooxygenases	47:60	arg1	Analysis					14:21	Bioinformatic Analysis	0:21	Bioinformatic Analysis of Lytic Polysaccharide Monooxygenases	0:60	Bioinformatic Analysis of Lytic Polysaccharide Monooxygenases Reveals the Pan-Families Occurrence of Intrinsically Disordered C-Terminal Extensions.
34827630	10	38	from	organisms	1478:1486	arg1	LPMOs					1437:1441	LPMOs	1437:1441	LPMOs from evolutionarily very divergent organisms	1437:1486	The widespread occurrence of dCTRs in LPMOs from evolutionarily very divergent organisms, hints at a possible functional role and opens new prospects in the field of LPMOs.
34827630	10	38	from	organisms	1478:1486	arg1	occurrence					1414:1423	The widespread occurrence	1399:1423	The widespread occurrence of dCTRs in LPMOs from evolutionarily very divergent organisms	1399:1486	The widespread occurrence of dCTRs in LPMOs from evolutionarily very divergent organisms, hints at a possible functional role and opens new prospects in the field of LPMOs.
34827630	2	39	theme	oxidative	275:283	arg1	cleavage					285:292	the oxidative cleavage	271:292	the oxidative cleavage of different types of polysaccharides	271:330	LPMOs catalyze the oxidative cleavage of different types of polysaccharides and are today divided into eight families (AA9-11, AA13-17) within the Auxiliary Activity enzyme class of the CAZy database.
34827630	2	40	theme	Auxiliary	403:411	arg1	class					429:433	the Auxiliary Activity enzyme class	399:433	the Auxiliary Activity enzyme class of the CAZy database	399:454	LPMOs catalyze the oxidative cleavage of different types of polysaccharides and are today divided into eight families (AA9-11, AA13-17) within the Auxiliary Activity enzyme class of the CAZy database.
34827630	6	41	theme	amino-acid	1038:1047	arg1	composition					1049:1059	amino-acid composition	1038:1059	amino-acid composition	1038:1059	Our analysis shows that these disordered C-terminal regions (dCTRs) are widespread in all LPMO families (except AA13) and differ in terms of sequence length and amino-acid composition.
34827630	0	42	theme	Polysaccharide	32:45	arg1	Monooxygenases					47:60	Lytic Polysaccharide Monooxygenases	26:60	Lytic Polysaccharide Monooxygenases	26:60	Bioinformatic Analysis of Lytic Polysaccharide Monooxygenases Reveals the Pan-Families Occurrence of Intrinsically Disordered C-Terminal Extensions.
34827630	6	43	theme	LPMO	967:970	arg1	families					972:979	all LPMO families	963:979	all LPMO families (except AA13)	963:993	Our analysis shows that these disordered C-terminal regions (dCTRs) are widespread in all LPMO families (except AA13) and differ in terms of sequence length and amino-acid composition.
34827630	9	44	from	AA11	1318:1321	arg1	dCTRs					1307:1311	dCTRs	1307:1311	dCTRs from AA11 and AA15	1307:1330	Interestingly, dCTRs from AA11 and AA15 are enriched in redox-sensitive, conditionally disordered regions.
34827630	5	45	theme	LPMO	775:778	arg1	families					780:787	different LPMO families	765:787	different LPMO families	765:787	Here, we analyzed 27,060 sequences from different LPMO families and show that 60% have a C-terminal extension predicted to be intrinsically disordered.
34827630	8	46	theme	polyampholytic	1155:1168	arg1	nature					1170:1175	a common polyampholytic nature	1146:1175	a common polyampholytic nature	1146:1175	LPMO-dCTRs share a common polyampholytic nature and an enrichment in serine and threonine residues, suggesting that they undergo post-translational modifications.
34827630	8	47	dep	serine	1198:1203	arg1	residues					1219:1226	residues	1219:1226	residues	1219:1226	LPMO-dCTRs share a common polyampholytic nature and an enrichment in serine and threonine residues, suggesting that they undergo post-translational modifications.
34827630	10	48	theme	LPMOs	1565:1569	arg1	field					1556:1560	the field	1552:1560	the field of LPMOs	1552:1569	The widespread occurrence of dCTRs in LPMOs from evolutionarily very divergent organisms, hints at a possible functional role and opens new prospects in the field of LPMOs.
34827630	1	49	theme	Lytic	149:153	arg1	monooxygenases					170:183	Lytic polysaccharide monooxygenases	149:183	Lytic polysaccharide monooxygenases (LPMOs)	149:191	Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes secreted by many organisms and viruses.
34827630	1	49	theme	Lytic	149:153	arg1	enzymes					208:214	monocopper enzymes	197:214	monocopper enzymes secreted by many organisms and viruses	197:253	Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes secreted by many organisms and viruses.
34827630	1	49	theme	Lytic	149:153	arg1	LPMOs					186:190	LPMOs	186:190	LPMOs	186:190	Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes secreted by many organisms and viruses.
34827630	8	50	from	nature	1170:1175	arg1	threonine					1209:1217	threonine	1209:1217	threonine	1209:1217	LPMO-dCTRs share a common polyampholytic nature and an enrichment in serine and threonine residues, suggesting that they undergo post-translational modifications.
34827630	8	50	from	nature	1170:1175	arg1	serine					1198:1203	serine	1198:1203	serine	1198:1203	LPMO-dCTRs share a common polyampholytic nature and an enrichment in serine and threonine residues, suggesting that they undergo post-translational modifications.
34827630	6	51	from	widespread	949:958	arg1	families					972:979	all LPMO families	963:979	all LPMO families (except AA13)	963:993	Our analysis shows that these disordered C-terminal regions (dCTRs) are widespread in all LPMO families (except AA13) and differ in terms of sequence length and amino-acid composition.
34827630	5	52	contain	have	807:810	arg2	extension					825:833	a C-terminal extension	812:833	a C-terminal extension predicted to be intrinsically disordered	812:874	Here, we analyzed 27,060 sequences from different LPMO families and show that 60% have a C-terminal extension predicted to be intrinsically disordered.
34827630	5	52	contain	have	807:810	arg1	%					805:805	60%	803:805	60%	803:805	Here, we analyzed 27,060 sequences from different LPMO families and show that 60% have a C-terminal extension predicted to be intrinsically disordered.
34827630	1	53	theme	polysaccharide	155:168	arg1	monooxygenases					170:183	Lytic polysaccharide monooxygenases	149:183	Lytic polysaccharide monooxygenases (LPMOs)	149:191	Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes secreted by many organisms and viruses.
34827630	1	53	theme	polysaccharide	155:168	arg1	enzymes					208:214	monocopper enzymes	197:214	monocopper enzymes secreted by many organisms and viruses	197:253	Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes secreted by many organisms and viruses.
34827630	1	53	theme	polysaccharide	155:168	arg1	LPMOs					186:190	LPMOs	186:190	LPMOs	186:190	Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes secreted by many organisms and viruses.
34827630	4	54	theme	known	701:705	arg1	function					707:714	any known function	697:714	any known function	697:714	Intriguingly, we observed that some LPMO sequences also display a C-terminal extension of varying length not associated with any known function or fold.
34827630	2	55	theme	types	307:311	arg1	cleavage					285:292	the oxidative cleavage	271:292	the oxidative cleavage of different types of polysaccharides	271:330	LPMOs catalyze the oxidative cleavage of different types of polysaccharides and are today divided into eight families (AA9-11, AA13-17) within the Auxiliary Activity enzyme class of the CAZy database.
34827630	4	56	theme	C-terminal	638:647	arg1	extension					649:657	a C-terminal extension	636:657	a C-terminal extension of varying length not associated with any known function or fold	636:722	Intriguingly, we observed that some LPMO sequences also display a C-terminal extension of varying length not associated with any known function or fold.
34827630	10	57	theme	new	1535:1537	arg1	prospects					1539:1547	new prospects	1535:1547	new prospects in the field of LPMOs	1535:1569	The widespread occurrence of dCTRs in LPMOs from evolutionarily very divergent organisms, hints at a possible functional role and opens new prospects in the field of LPMOs.
34827630	5	58	from	families	780:787	arg1	sequences					750:758	27,060 sequences	743:758	27,060 sequences from different LPMO families	743:787	Here, we analyzed 27,060 sequences from different LPMO families and show that 60% have a C-terminal extension predicted to be intrinsically disordered.
34827630	2	59	theme	different	297:305	arg1	types					307:311	different types	297:311	different types of polysaccharides	297:330	LPMOs catalyze the oxidative cleavage of different types of polysaccharides and are today divided into eight families (AA9-11, AA13-17) within the Auxiliary Activity enzyme class of the CAZy database.
34827630	6	60	theme	sequence	1018:1025	arg1	length					1027:1032	sequence length	1018:1032	sequence length	1018:1032	Our analysis shows that these disordered C-terminal regions (dCTRs) are widespread in all LPMO families (except AA13) and differ in terms of sequence length and amino-acid composition.
34827630	3	61	theme	catalytic	498:506	arg1	domain					508:513	a catalytic domain	496:513	a catalytic domain	496:513	LPMOs minimal architecture encompasses a catalytic domain, to which can be appended a carbohydrate-binding module.
34827630	0	62	theme	Pan-Families	74:85	arg1	Occurrence					87:96	the Pan-Families Occurrence	70:96	the Pan-Families Occurrence of Intrinsically Disordered C-Terminal Extensions	70:146	Bioinformatic Analysis of Lytic Polysaccharide Monooxygenases Reveals the Pan-Families Occurrence of Intrinsically Disordered C-Terminal Extensions.
34827630	4	63	theme	length	670:675	arg1	extension					649:657	a C-terminal extension	636:657	a C-terminal extension of varying length not associated with any known function or fold	636:722	Intriguingly, we observed that some LPMO sequences also display a C-terminal extension of varying length not associated with any known function or fold.
34827630	3	64	theme	carbohydrate-binding	543:562	arg1	module					564:569	a carbohydrate-binding module	541:569	a carbohydrate-binding module	541:569	LPMOs minimal architecture encompasses a catalytic domain, to which can be appended a carbohydrate-binding module.
34827630	8	65	theme	common	1148:1153	arg1	nature					1170:1175	a common polyampholytic nature	1146:1175	a common polyampholytic nature	1146:1175	LPMO-dCTRs share a common polyampholytic nature and an enrichment in serine and threonine residues, suggesting that they undergo post-translational modifications.
32895176	12	0	theme	low	2017:2019	arg1	dose					2051:2054	the low (0.1 g/kg) or high (0.4 g/kg) dose	2013:2054	the low (0.1 g/kg) or high (0.4 g/kg) dose	2013:2054	The glucose-lowering effects of TOPS-1 was not significant at the low (0.1 g/kg) or high (0.4 g/kg) dose, and a high dose tended to increase the mortality of the mice.
32895176	5	1	with	fractions	678:686	arg1	signals					735:741	consistent UV detection signals	711:741	consistent UV detection signals	711:741	The fractions TOPW-1 and TOPS-1 with consistent UV detection signals were collected and HPGPC was used to determine their relative molecular mass.
32895176	11	2	theme	total	1840:1844	arg1	cholesterol					1846:1856	total cholesterol	1840:1856	total cholesterol	1840:1856	In the mouse model of hyperglycemia and hyperlipidemia, treatment with TOPS-1 (0.2 g/kg) enhanced the activity of lipase and significantly reduced fasting glucose level and serum contents of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol without causing death in the mice.
32895176	12	3	theme	TOPS-1	1983:1988	arg1	significant					1998:2008	significant	1998:2008	significant	1998:2008	The glucose-lowering effects of TOPS-1 was not significant at the low (0.1 g/kg) or high (0.4 g/kg) dose, and a high dose tended to increase the mortality of the mice.
32895176	12	3	theme	TOPS-1	1983:1988	arg1	effects					1972:1978	The glucose-lowering effects	1951:1978	The glucose-lowering effects of TOPS-1	1951:1988	The glucose-lowering effects of TOPS-1 was not significant at the low (0.1 g/kg) or high (0.4 g/kg) dose, and a high dose tended to increase the mortality of the mice.
32895176	4	4	theme	polysaccharide	614:627	arg1	fractions					629:637	the polysaccharide fractions	610:637	the polysaccharide fractions	610:637	The anti-oxidation activity of the polysaccharide fractions was investigated with ABTS method.
32895176	2	5	theme	H2O	316:318	arg1	solution					339:346	H2O and 0.5 mol/L NaCl solution	316:346	H2O and 0.5 mol/L NaCl solution	316:346	METHODS The crude polysaccharides of T. orientalis were chromatographed on DEAE cellulose column using H2O and 0.5 mol/L NaCl solution as the eluent and DEAE-water and DEAE-salt with high polysaccharide contents were collected.
32895176	11	6	theme	triacylglycerol	1859:1873	arg1	level					1812:1816	fasting glucose level	1796:1816	fasting glucose level	1796:1816	In the mouse model of hyperglycemia and hyperlipidemia, treatment with TOPS-1 (0.2 g/kg) enhanced the activity of lipase and significantly reduced fasting glucose level and serum contents of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol without causing death in the mice.
32895176	11	6	theme	triacylglycerol	1859:1873	arg1	contents					1828:1835	serum contents	1822:1835	serum contents	1822:1835	In the mouse model of hyperglycemia and hyperlipidemia, treatment with TOPS-1 (0.2 g/kg) enhanced the activity of lipase and significantly reduced fasting glucose level and serum contents of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol without causing death in the mice.
32895176	8	7	with	comparison	1289:1298	arg1	g/kg					1356:1359	0.5 g/kg	1352:1359	0.5 g/kg	1352:1359	In a mouse model of hyperglycemic and hyperlipidemic, the glucose-lowering effect of TOPS-1 (0.1, 0.2, and 0.4 g/kg) and its effect on blood lipid metabolism were investigated in comparison with Xiaoke pills (5 pills/kg) and Danshen tablets (0.5 g/kg).
32895176	8	7	with	comparison	1289:1298	arg1	pills					1312:1316	Xiaoke pills	1305:1316	Xiaoke pills (5 pills/kg)	1305:1329	In a mouse model of hyperglycemic and hyperlipidemic, the glucose-lowering effect of TOPS-1 (0.1, 0.2, and 0.4 g/kg) and its effect on blood lipid metabolism were investigated in comparison with Xiaoke pills (5 pills/kg) and Danshen tablets (0.5 g/kg).
32895176	8	7	with	comparison	1289:1298	arg1	pills/kg					1321:1328	5 pills/kg	1319:1328	5 pills/kg	1319:1328	In a mouse model of hyperglycemic and hyperlipidemic, the glucose-lowering effect of TOPS-1 (0.1, 0.2, and 0.4 g/kg) and its effect on blood lipid metabolism were investigated in comparison with Xiaoke pills (5 pills/kg) and Danshen tablets (0.5 g/kg).
32895176	8	7	with	comparison	1289:1298	arg1	tablets					1343:1349	Danshen tablets	1335:1349	Danshen tablets (0.5 g/kg)	1335:1360	In a mouse model of hyperglycemic and hyperlipidemic, the glucose-lowering effect of TOPS-1 (0.1, 0.2, and 0.4 g/kg) and its effect on blood lipid metabolism were investigated in comparison with Xiaoke pills (5 pills/kg) and Danshen tablets (0.5 g/kg).
32895176	6	8	theme	monosaccharide	825:838	arg1	composition					840:850	The monosaccharide composition	821:850	The monosaccharide composition in homogeneous TOPW-1	821:872	The monosaccharide composition in homogeneous TOPW-1 was determined by acid hydrolysis combined with HPLC.
32895176	1	9	theme	Trametes	192:199	arg1	orientalis					201:210	Trametes orientalis	192:210	Trametes orientalis	192:210	OBJECTIVE To explore the glucose-lowering effect of the polysaccharide fractions of Trametes orientalis.
32895176	10	10	theme	μg/mL	1598:1602	arg1	polysaccharide					1551:1564	a single polysaccharide	1542:1564	a single polysaccharide	1542:1564	TOPS-1 was not a single polysaccharide and at the concentration of 500 μg/mL showed an high ABTS clearance rate (90.15%).
32895176	10	10	theme	μg/mL	1598:1602	arg1	TOPS-1					1527:1532	TOPS-1	1527:1532	TOPS-1	1527:1532	TOPS-1 was not a single polysaccharide and at the concentration of 500 μg/mL showed an high ABTS clearance rate (90.15%).
32895176	10	10	theme	μg/mL	1598:1602	arg1	concentration					1577:1589	the concentration	1573:1589	the concentration of 500 μg/mL	1573:1602	TOPS-1 was not a single polysaccharide and at the concentration of 500 μg/mL showed an high ABTS clearance rate (90.15%).
32895176	11	11	theme	lipase	1763:1768	arg1	activity					1751:1758	the activity	1747:1758	the activity of lipase	1747:1768	In the mouse model of hyperglycemia and hyperlipidemia, treatment with TOPS-1 (0.2 g/kg) enhanced the activity of lipase and significantly reduced fasting glucose level and serum contents of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol without causing death in the mice.
32895176	7	12	dep	in	932:933	arg1	vitro					935:939	vitro	935:939	vitro	935:939	The in vitro inhibitory activities of TOPW-1 and TOPS-1 against DPP4, adipocyte glucose intake and lipase activity were tested to preliminarily assess their glucose-lowering effect.
32895176	2	13	theme	NaCl	334:337	arg1	solution					339:346	H2O and 0.5 mol/L NaCl solution	316:346	H2O and 0.5 mol/L NaCl solution	316:346	METHODS The crude polysaccharides of T. orientalis were chromatographed on DEAE cellulose column using H2O and 0.5 mol/L NaCl solution as the eluent and DEAE-water and DEAE-salt with high polysaccharide contents were collected.
32895176	2	14	theme	0.5	324:326	arg1	mol/L					328:332	mol/L	328:332	mol/L	328:332	METHODS The crude polysaccharides of T. orientalis were chromatographed on DEAE cellulose column using H2O and 0.5 mol/L NaCl solution as the eluent and DEAE-water and DEAE-salt with high polysaccharide contents were collected.
32895176	13	15	theme	glucose-lowering	2245:2260	arg1	effect					2262:2267	their glucose-lowering effect	2239:2267	their glucose-lowering effect	2239:2267	CONCLUSIONS T. orientalis polysaccharides have anti-oxidation, glucose-lowering and lipid-lowering effects in mice, and their glucose-lowering effect is probably medicated by reducing oxidative stress and ameliorating lipid metabolism disorder.
32895176	8	16	theme	Xiaoke	1305:1310	arg1	pills					1312:1316	Xiaoke pills	1305:1316	Xiaoke pills (5 pills/kg)	1305:1329	In a mouse model of hyperglycemic and hyperlipidemic, the glucose-lowering effect of TOPS-1 (0.1, 0.2, and 0.4 g/kg) and its effect on blood lipid metabolism were investigated in comparison with Xiaoke pills (5 pills/kg) and Danshen tablets (0.5 g/kg).
32895176	8	16	theme	Xiaoke	1305:1310	arg1	pills/kg					1321:1328	5 pills/kg	1319:1328	5 pills/kg	1319:1328	In a mouse model of hyperglycemic and hyperlipidemic, the glucose-lowering effect of TOPS-1 (0.1, 0.2, and 0.4 g/kg) and its effect on blood lipid metabolism were investigated in comparison with Xiaoke pills (5 pills/kg) and Danshen tablets (0.5 g/kg).
32895176	10	17	theme	ABTS	1619:1622	arg1	rate					1634:1637	an high ABTS clearance rate	1611:1637	an high ABTS clearance rate (90.15%)	1611:1646	TOPS-1 was not a single polysaccharide and at the concentration of 500 μg/mL showed an high ABTS clearance rate (90.15%).
32895176	10	17	theme	ABTS	1619:1622	arg1	%					1645:1645	90.15%	1640:1645	90.15%	1640:1645	TOPS-1 was not a single polysaccharide and at the concentration of 500 μg/mL showed an high ABTS clearance rate (90.15%).
32895176	11	18	theme	fasting	1796:1802	arg1	level					1812:1816	fasting glucose level	1796:1816	fasting glucose level	1796:1816	In the mouse model of hyperglycemia and hyperlipidemia, treatment with TOPS-1 (0.2 g/kg) enhanced the activity of lipase and significantly reduced fasting glucose level and serum contents of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol without causing death in the mice.
32895176	2	19	theme	orientalis	253:262	arg1	polysaccharides					231:245	The crude polysaccharides	221:245	The crude polysaccharides of T. orientalis	221:262	METHODS The crude polysaccharides of T. orientalis were chromatographed on DEAE cellulose column using H2O and 0.5 mol/L NaCl solution as the eluent and DEAE-water and DEAE-salt with high polysaccharide contents were collected.
32895176	8	20	theme	blood	1245:1249	arg1	metabolism					1257:1266	blood lipid metabolism	1245:1266	blood lipid metabolism	1245:1266	In a mouse model of hyperglycemic and hyperlipidemic, the glucose-lowering effect of TOPS-1 (0.1, 0.2, and 0.4 g/kg) and its effect on blood lipid metabolism were investigated in comparison with Xiaoke pills (5 pills/kg) and Danshen tablets (0.5 g/kg).
32895176	5	21	theme	UV	722:723	arg1	signals					735:741	consistent UV detection signals	711:741	consistent UV detection signals	711:741	The fractions TOPW-1 and TOPS-1 with consistent UV detection signals were collected and HPGPC was used to determine their relative molecular mass.
32895176	12	22	theme	high	2035:2038	arg1	dose					2051:2054	the low (0.1 g/kg) or high (0.4 g/kg) dose	2013:2054	the low (0.1 g/kg) or high (0.4 g/kg) dose	2013:2054	The glucose-lowering effects of TOPS-1 was not significant at the low (0.1 g/kg) or high (0.4 g/kg) dose, and a high dose tended to increase the mortality of the mice.
32895176	5	23	theme	consistent	711:720	arg1	signals					735:741	consistent UV detection signals	711:741	consistent UV detection signals	711:741	The fractions TOPW-1 and TOPS-1 with consistent UV detection signals were collected and HPGPC was used to determine their relative molecular mass.
32895176	9	24	theme	antioxidant	1489:1499	arg1	effects					1518:1524	weak antioxidant and hypoglycemic effects	1484:1524	weak antioxidant and hypoglycemic effects	1484:1524	RESULTS TOPW-1 was a homogeneous polysaccharide composed mainly of D-mannose, D-glucose, D-galactose, and D-fucose, with weak antioxidant and hypoglycemic effects.
32895176	7	25	theme	glucose-lowering	1085:1100	arg1	effect					1102:1107	their glucose-lowering effect	1079:1107	their glucose-lowering effect	1079:1107	The in vitro inhibitory activities of TOPW-1 and TOPS-1 against DPP4, adipocyte glucose intake and lipase activity were tested to preliminarily assess their glucose-lowering effect.
32895176	2	26	theme	DEAE	288:291	arg1	column					303:308	DEAE cellulose column	288:308	DEAE cellulose column using H2O and 0.5 mol/L NaCl solution as the eluent and DEAE-water and DEAE-salt with high polysaccharide contents	288:423	METHODS The crude polysaccharides of T. orientalis were chromatographed on DEAE cellulose column using H2O and 0.5 mol/L NaCl solution as the eluent and DEAE-water and DEAE-salt with high polysaccharide contents were collected.
32895176	9	27	theme	hypoglycemic	1505:1516	arg1	effects					1518:1524	weak antioxidant and hypoglycemic effects	1484:1524	weak antioxidant and hypoglycemic effects	1484:1524	RESULTS TOPW-1 was a homogeneous polysaccharide composed mainly of D-mannose, D-glucose, D-galactose, and D-fucose, with weak antioxidant and hypoglycemic effects.
32895176	0	28	theme	hyperglycemic	68:80	arg1	mice					101:104	hyperglycemic and hyperlipidemic mice	68:104	hyperglycemic and hyperlipidemic mice	68:104	[Glucose- lowering effect of Trametes orientalis polysaccharides in hyperglycemic and hyperlipidemic mice].
32895176	0	29	from	effect	19:24	arg1	mice					101:104	hyperglycemic and hyperlipidemic mice	68:104	hyperglycemic and hyperlipidemic mice	68:104	[Glucose- lowering effect of Trametes orientalis polysaccharides in hyperglycemic and hyperlipidemic mice].
32895176	5	30	theme	detection	725:733	arg1	signals					735:741	consistent UV detection signals	711:741	consistent UV detection signals	711:741	The fractions TOPW-1 and TOPS-1 with consistent UV detection signals were collected and HPGPC was used to determine their relative molecular mass.
32895176	0	31	theme	hyperlipidemic	86:99	arg1	mice					101:104	hyperglycemic and hyperlipidemic mice	68:104	hyperglycemic and hyperlipidemic mice	68:104	[Glucose- lowering effect of Trametes orientalis polysaccharides in hyperglycemic and hyperlipidemic mice].
32895176	5	32	used	used	772:775	arg2	HPGPC					762:766	HPGPC	762:766	HPGPC	762:766	The fractions TOPW-1 and TOPS-1 with consistent UV detection signals were collected and HPGPC was used to determine their relative molecular mass.
32895176	7	33	theme	glucose	1008:1014	arg1	intake					1016:1021	adipocyte glucose intake	998:1021	adipocyte glucose intake	998:1021	The in vitro inhibitory activities of TOPW-1 and TOPS-1 against DPP4, adipocyte glucose intake and lipase activity were tested to preliminarily assess their glucose-lowering effect.
32895176	1	34	theme	fractions	179:187	arg1	effect					150:155	the glucose-lowering effect	129:155	the glucose-lowering effect of the polysaccharide fractions of Trametes orientalis	129:210	OBJECTIVE To explore the glucose-lowering effect of the polysaccharide fractions of Trametes orientalis.
32895176	6	35	from	composition	840:850	arg1	TOPW-1					867:872	homogeneous TOPW-1	855:872	homogeneous TOPW-1	855:872	The monosaccharide composition in homogeneous TOPW-1 was determined by acid hydrolysis combined with HPLC.
32895176	2	36	theme	polysaccharide	401:414	arg1	contents					416:423	high polysaccharide contents	396:423	high polysaccharide contents	396:423	METHODS The crude polysaccharides of T. orientalis were chromatographed on DEAE cellulose column using H2O and 0.5 mol/L NaCl solution as the eluent and DEAE-water and DEAE-salt with high polysaccharide contents were collected.
32895176	0	37	theme	[Glucose-	0:8	arg1	effect					19:24	[Glucose- lowering effect	0:24	[Glucose- lowering effect of Trametes orientalis polysaccharides in hyperglycemic and hyperlipidemic mice	0:104	[Glucose- lowering effect of Trametes orientalis polysaccharides in hyperglycemic and hyperlipidemic mice].
32895176	8	38	theme	mouse	1115:1119	arg1	model					1121:1125	a mouse model	1113:1125	a mouse model of hyperglycemic and hyperlipidemic	1113:1161	In a mouse model of hyperglycemic and hyperlipidemic, the glucose-lowering effect of TOPS-1 (0.1, 0.2, and 0.4 g/kg) and its effect on blood lipid metabolism were investigated in comparison with Xiaoke pills (5 pills/kg) and Danshen tablets (0.5 g/kg).
32895176	11	39	theme	hyperglycemia	1671:1683	arg1	model					1662:1666	the mouse model	1652:1666	the mouse model of hyperglycemia and hyperlipidemia	1652:1702	In the mouse model of hyperglycemia and hyperlipidemia, treatment with TOPS-1 (0.2 g/kg) enhanced the activity of lipase and significantly reduced fasting glucose level and serum contents of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol without causing death in the mice.
32895176	5	40	dep	fractions	678:686	arg1	TOPW-1					688:693	TOPW-1	688:693	TOPW-1	688:693	The fractions TOPW-1 and TOPS-1 with consistent UV detection signals were collected and HPGPC was used to determine their relative molecular mass.
32895176	5	40	dep	fractions	678:686	arg1	fractions					678:686	The fractions TOPW-1 and TOPS-1	674:704	The fractions TOPW-1 and TOPS-1 with consistent UV detection signals	674:741	The fractions TOPW-1 and TOPS-1 with consistent UV detection signals were collected and HPGPC was used to determine their relative molecular mass.
32895176	5	40	dep	fractions	678:686	arg1	TOPS-1					699:704	TOPS-1	699:704	TOPS-1	699:704	The fractions TOPW-1 and TOPS-1 with consistent UV detection signals were collected and HPGPC was used to determine their relative molecular mass.
32895176	3	41	theme	gel	495:497	arg1	column					499:504	Sephadex G-100 gel column	480:504	Sephadex G-100 gel column	480:504	The two fractions were separated using Sephadex G-100 gel column to obtain 4 polysaccharide fractions TOPW-1, TOPW-2, TOPS-1, and TOPS-2.
32895176	13	42	theme	anti-oxidation	2166:2179	arg1	effects					2218:2224	anti-oxidation, glucose-lowering and lipid-lowering effects	2166:2224	anti-oxidation, glucose-lowering and lipid-lowering effects	2166:2224	CONCLUSIONS T. orientalis polysaccharides have anti-oxidation, glucose-lowering and lipid-lowering effects in mice, and their glucose-lowering effect is probably medicated by reducing oxidative stress and ameliorating lipid metabolism disorder.
32895176	3	43	theme	Sephadex	480:487	arg1	column					499:504	Sephadex G-100 gel column	480:504	Sephadex G-100 gel column	480:504	The two fractions were separated using Sephadex G-100 gel column to obtain 4 polysaccharide fractions TOPW-1, TOPW-2, TOPS-1, and TOPS-2.
32895176	11	44	theme	lipoprotein	1892:1902	arg1	cholesterol					1904:1914	low-density lipoprotein cholesterol	1880:1914	low-density lipoprotein cholesterol	1880:1914	In the mouse model of hyperglycemia and hyperlipidemia, treatment with TOPS-1 (0.2 g/kg) enhanced the activity of lipase and significantly reduced fasting glucose level and serum contents of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol without causing death in the mice.
32895176	0	45	theme	Trametes	29:36	arg1	polysaccharides					49:63	Trametes orientalis polysaccharides	29:63	Trametes orientalis polysaccharides	29:63	[Glucose- lowering effect of Trametes orientalis polysaccharides in hyperglycemic and hyperlipidemic mice].
32895176	13	46	theme	metabolism	2343:2352	arg1	disorder					2354:2361	lipid metabolism disorder	2337:2361	lipid metabolism disorder	2337:2361	CONCLUSIONS T. orientalis polysaccharides have anti-oxidation, glucose-lowering and lipid-lowering effects in mice, and their glucose-lowering effect is probably medicated by reducing oxidative stress and ameliorating lipid metabolism disorder.
32895176	12	47	dep	low	2017:2019	arg1	g/kg					2026:2029	0.1 g/kg	2022:2029	0.1 g/kg	2022:2029	The glucose-lowering effects of TOPS-1 was not significant at the low (0.1 g/kg) or high (0.4 g/kg) dose, and a high dose tended to increase the mortality of the mice.
32895176	12	48	theme	mice	2113:2116	arg1	mortality					2096:2104	the mortality	2092:2104	the mortality of the mice	2092:2116	The glucose-lowering effects of TOPS-1 was not significant at the low (0.1 g/kg) or high (0.4 g/kg) dose, and a high dose tended to increase the mortality of the mice.
32895176	11	49	theme	serum	1822:1826	arg1	contents					1828:1835	serum contents	1822:1835	serum contents	1822:1835	In the mouse model of hyperglycemia and hyperlipidemia, treatment with TOPS-1 (0.2 g/kg) enhanced the activity of lipase and significantly reduced fasting glucose level and serum contents of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol without causing death in the mice.
32895176	0	50	theme	polysaccharides	49:63	arg1	effect					19:24	[Glucose- lowering effect	0:24	[Glucose- lowering effect of Trametes orientalis polysaccharides in hyperglycemic and hyperlipidemic mice	0:104	[Glucose- lowering effect of Trametes orientalis polysaccharides in hyperglycemic and hyperlipidemic mice].
32895176	6	51	theme	acid	892:895	arg1	hydrolysis					897:906	acid hydrolysis	892:906	acid hydrolysis combined with HPLC	892:925	The monosaccharide composition in homogeneous TOPW-1 was determined by acid hydrolysis combined with HPLC.
32895176	2	52	theme	crude	225:229	arg1	polysaccharides					231:245	The crude polysaccharides	221:245	The crude polysaccharides of T. orientalis	221:262	METHODS The crude polysaccharides of T. orientalis were chromatographed on DEAE cellulose column using H2O and 0.5 mol/L NaCl solution as the eluent and DEAE-water and DEAE-salt with high polysaccharide contents were collected.
32895176	7	53	theme	TOPW-1	966:971	arg1	activities					952:961	The in vitro inhibitory activities	928:961	The in vitro inhibitory activities of TOPW-1 and TOPS-1 against DPP4, adipocyte glucose intake and lipase activity	928:1041	The in vitro inhibitory activities of TOPW-1 and TOPS-1 against DPP4, adipocyte glucose intake and lipase activity were tested to preliminarily assess their glucose-lowering effect.
32895176	8	54	theme	glucose-lowering	1168:1183	arg1	effect					1185:1190	the glucose-lowering effect	1164:1190	the glucose-lowering effect of TOPS-1 (0.1, 0.2, and 0.4 g/kg)	1164:1225	In a mouse model of hyperglycemic and hyperlipidemic, the glucose-lowering effect of TOPS-1 (0.1, 0.2, and 0.4 g/kg) and its effect on blood lipid metabolism were investigated in comparison with Xiaoke pills (5 pills/kg) and Danshen tablets (0.5 g/kg).
32895176	4	55	theme	fractions	629:637	arg1	activity					598:605	The anti-oxidation activity	579:605	The anti-oxidation activity of the polysaccharide fractions	579:637	The anti-oxidation activity of the polysaccharide fractions was investigated with ABTS method.
32895176	11	56	theme	cholesterol	1904:1914	arg1	level					1812:1816	fasting glucose level	1796:1816	fasting glucose level	1796:1816	In the mouse model of hyperglycemia and hyperlipidemia, treatment with TOPS-1 (0.2 g/kg) enhanced the activity of lipase and significantly reduced fasting glucose level and serum contents of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol without causing death in the mice.
32895176	11	56	theme	cholesterol	1904:1914	arg1	contents					1828:1835	serum contents	1822:1835	serum contents	1822:1835	In the mouse model of hyperglycemia and hyperlipidemia, treatment with TOPS-1 (0.2 g/kg) enhanced the activity of lipase and significantly reduced fasting glucose level and serum contents of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol without causing death in the mice.
32895176	11	57	theme	cholesterol	1846:1856	arg1	level					1812:1816	fasting glucose level	1796:1816	fasting glucose level	1796:1816	In the mouse model of hyperglycemia and hyperlipidemia, treatment with TOPS-1 (0.2 g/kg) enhanced the activity of lipase and significantly reduced fasting glucose level and serum contents of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol without causing death in the mice.
32895176	11	57	theme	cholesterol	1846:1856	arg1	contents					1828:1835	serum contents	1822:1835	serum contents	1822:1835	In the mouse model of hyperglycemia and hyperlipidemia, treatment with TOPS-1 (0.2 g/kg) enhanced the activity of lipase and significantly reduced fasting glucose level and serum contents of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol without causing death in the mice.
32895176	6	58	theme	homogeneous	855:865	arg1	TOPW-1					867:872	homogeneous TOPW-1	855:872	homogeneous TOPW-1	855:872	The monosaccharide composition in homogeneous TOPW-1 was determined by acid hydrolysis combined with HPLC.
32895176	13	59	contain	have	2161:2164	arg2	effects					2218:2224	anti-oxidation, glucose-lowering and lipid-lowering effects	2166:2224	anti-oxidation, glucose-lowering and lipid-lowering effects	2166:2224	CONCLUSIONS T. orientalis polysaccharides have anti-oxidation, glucose-lowering and lipid-lowering effects in mice, and their glucose-lowering effect is probably medicated by reducing oxidative stress and ameliorating lipid metabolism disorder.
32895176	13	59	contain	have	2161:2164	arg1	CONCLUSIONS					2119:2129	CONCLUSIONS	2119:2129	CONCLUSIONS T. orientalis polysaccharides	2119:2159	CONCLUSIONS T. orientalis polysaccharides have anti-oxidation, glucose-lowering and lipid-lowering effects in mice, and their glucose-lowering effect is probably medicated by reducing oxidative stress and ameliorating lipid metabolism disorder.
32895176	13	60	theme	lipid-lowering	2203:2216	arg1	effects					2218:2224	anti-oxidation, glucose-lowering and lipid-lowering effects	2166:2224	anti-oxidation, glucose-lowering and lipid-lowering effects	2166:2224	CONCLUSIONS T. orientalis polysaccharides have anti-oxidation, glucose-lowering and lipid-lowering effects in mice, and their glucose-lowering effect is probably medicated by reducing oxidative stress and ameliorating lipid metabolism disorder.
32895176	8	61	from	effect	1235:1240	arg1	metabolism					1257:1266	blood lipid metabolism	1245:1266	blood lipid metabolism	1245:1266	In a mouse model of hyperglycemic and hyperlipidemic, the glucose-lowering effect of TOPS-1 (0.1, 0.2, and 0.4 g/kg) and its effect on blood lipid metabolism were investigated in comparison with Xiaoke pills (5 pills/kg) and Danshen tablets (0.5 g/kg).
32895176	8	62	theme	lipid	1251:1255	arg1	metabolism					1257:1266	blood lipid metabolism	1245:1266	blood lipid metabolism	1245:1266	In a mouse model of hyperglycemic and hyperlipidemic, the glucose-lowering effect of TOPS-1 (0.1, 0.2, and 0.4 g/kg) and its effect on blood lipid metabolism were investigated in comparison with Xiaoke pills (5 pills/kg) and Danshen tablets (0.5 g/kg).
32895176	5	63	theme	molecular	805:813	arg1	mass					815:818	their relative molecular mass	790:818	their relative molecular mass	790:818	The fractions TOPW-1 and TOPS-1 with consistent UV detection signals were collected and HPGPC was used to determine their relative molecular mass.
32895176	11	64	with	treatment	1705:1713	arg1	TOPS-1					1720:1725	TOPS-1	1720:1725	TOPS-1 (0.2 g/kg)	1720:1736	In the mouse model of hyperglycemia and hyperlipidemia, treatment with TOPS-1 (0.2 g/kg) enhanced the activity of lipase and significantly reduced fasting glucose level and serum contents of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol without causing death in the mice.
32895176	11	64	with	treatment	1705:1713	arg1	g/kg					1732:1735	0.2 g/kg	1728:1735	0.2 g/kg	1728:1735	In the mouse model of hyperglycemia and hyperlipidemia, treatment with TOPS-1 (0.2 g/kg) enhanced the activity of lipase and significantly reduced fasting glucose level and serum contents of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol without causing death in the mice.
32895176	1	65	theme	orientalis	201:210	arg1	fractions					179:187	the polysaccharide fractions	160:187	the polysaccharide fractions of Trametes orientalis	160:210	OBJECTIVE To explore the glucose-lowering effect of the polysaccharide fractions of Trametes orientalis.
32895176	11	66	from	death	1932:1936	arg1	mice					1945:1948	the mice	1941:1948	the mice	1941:1948	In the mouse model of hyperglycemia and hyperlipidemia, treatment with TOPS-1 (0.2 g/kg) enhanced the activity of lipase and significantly reduced fasting glucose level and serum contents of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol without causing death in the mice.
32895176	12	67	theme	high	2063:2066	arg1	dose					2068:2071	a high dose	2061:2071	a high dose	2061:2071	The glucose-lowering effects of TOPS-1 was not significant at the low (0.1 g/kg) or high (0.4 g/kg) dose, and a high dose tended to increase the mortality of the mice.
32895176	2	68	theme	mol/L	328:332	arg1	NaCl					334:337	0.5 mol/L NaCl	324:337	0.5 mol/L NaCl	324:337	METHODS The crude polysaccharides of T. orientalis were chromatographed on DEAE cellulose column using H2O and 0.5 mol/L NaCl solution as the eluent and DEAE-water and DEAE-salt with high polysaccharide contents were collected.
32895176	12	69	theme	glucose-lowering	1955:1970	arg1	significant					1998:2008	significant	1998:2008	significant	1998:2008	The glucose-lowering effects of TOPS-1 was not significant at the low (0.1 g/kg) or high (0.4 g/kg) dose, and a high dose tended to increase the mortality of the mice.
32895176	12	69	theme	glucose-lowering	1955:1970	arg1	effects					1972:1978	The glucose-lowering effects	1951:1978	The glucose-lowering effects of TOPS-1	1951:1988	The glucose-lowering effects of TOPS-1 was not significant at the low (0.1 g/kg) or high (0.4 g/kg) dose, and a high dose tended to increase the mortality of the mice.
32895176	4	70	theme	ABTS	661:664	arg1	method					666:671	ABTS method	661:671	ABTS method	661:671	The anti-oxidation activity of the polysaccharide fractions was investigated with ABTS method.
32895176	10	71	theme	clearance	1624:1632	arg1	rate					1634:1637	an high ABTS clearance rate	1611:1637	an high ABTS clearance rate (90.15%)	1611:1646	TOPS-1 was not a single polysaccharide and at the concentration of 500 μg/mL showed an high ABTS clearance rate (90.15%).
32895176	10	71	theme	clearance	1624:1632	arg1	%					1645:1645	90.15%	1640:1645	90.15%	1640:1645	TOPS-1 was not a single polysaccharide and at the concentration of 500 μg/mL showed an high ABTS clearance rate (90.15%).
32895176	10	72	theme	high	1614:1617	arg1	rate					1634:1637	an high ABTS clearance rate	1611:1637	an high ABTS clearance rate (90.15%)	1611:1646	TOPS-1 was not a single polysaccharide and at the concentration of 500 μg/mL showed an high ABTS clearance rate (90.15%).
32895176	10	72	theme	high	1614:1617	arg1	%					1645:1645	90.15%	1640:1645	90.15%	1640:1645	TOPS-1 was not a single polysaccharide and at the concentration of 500 μg/mL showed an high ABTS clearance rate (90.15%).
32895176	11	73	theme	glucose	1804:1810	arg1	level					1812:1816	fasting glucose level	1796:1816	fasting glucose level	1796:1816	In the mouse model of hyperglycemia and hyperlipidemia, treatment with TOPS-1 (0.2 g/kg) enhanced the activity of lipase and significantly reduced fasting glucose level and serum contents of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol without causing death in the mice.
32895176	2	74	theme	T.	250:251	arg1	orientalis					253:262	T. orientalis	250:262	T. orientalis	250:262	METHODS The crude polysaccharides of T. orientalis were chromatographed on DEAE cellulose column using H2O and 0.5 mol/L NaCl solution as the eluent and DEAE-water and DEAE-salt with high polysaccharide contents were collected.
32895176	9	75	theme	weak	1484:1487	arg1	effects					1518:1524	weak antioxidant and hypoglycemic effects	1484:1524	weak antioxidant and hypoglycemic effects	1484:1524	RESULTS TOPW-1 was a homogeneous polysaccharide composed mainly of D-mannose, D-glucose, D-galactose, and D-fucose, with weak antioxidant and hypoglycemic effects.
32895176	10	76	dep	polysaccharide	1551:1564	arg1	showed					1604:1609	showed	1604:1609	showed an high ABTS clearance rate (90.15%)	1604:1646	TOPS-1 was not a single polysaccharide and at the concentration of 500 μg/mL showed an high ABTS clearance rate (90.15%).
32895176	2	77	theme	cellulose	293:301	arg1	column					303:308	DEAE cellulose column	288:308	DEAE cellulose column using H2O and 0.5 mol/L NaCl solution as the eluent and DEAE-water and DEAE-salt with high polysaccharide contents	288:423	METHODS The crude polysaccharides of T. orientalis were chromatographed on DEAE cellulose column using H2O and 0.5 mol/L NaCl solution as the eluent and DEAE-water and DEAE-salt with high polysaccharide contents were collected.
32895176	1	78	theme	glucose-lowering	133:148	arg1	effect					150:155	the glucose-lowering effect	129:155	the glucose-lowering effect of the polysaccharide fractions of Trametes orientalis	129:210	OBJECTIVE To explore the glucose-lowering effect of the polysaccharide fractions of Trametes orientalis.
32895176	13	79	theme	glucose-lowering	2182:2197	arg1	effects					2218:2224	anti-oxidation, glucose-lowering and lipid-lowering effects	2166:2224	anti-oxidation, glucose-lowering and lipid-lowering effects	2166:2224	CONCLUSIONS T. orientalis polysaccharides have anti-oxidation, glucose-lowering and lipid-lowering effects in mice, and their glucose-lowering effect is probably medicated by reducing oxidative stress and ameliorating lipid metabolism disorder.
32895176	5	80	theme	relative	796:803	arg1	mass					815:818	their relative molecular mass	790:818	their relative molecular mass	790:818	The fractions TOPW-1 and TOPS-1 with consistent UV detection signals were collected and HPGPC was used to determine their relative molecular mass.
32895176	7	81	theme	lipase	1027:1032	arg1	activity					1034:1041	lipase activity	1027:1041	lipase activity	1027:1041	The in vitro inhibitory activities of TOPW-1 and TOPS-1 against DPP4, adipocyte glucose intake and lipase activity were tested to preliminarily assess their glucose-lowering effect.
32895176	1	82	theme	polysaccharide	164:177	arg1	fractions					179:187	the polysaccharide fractions	160:187	the polysaccharide fractions of Trametes orientalis	160:210	OBJECTIVE To explore the glucose-lowering effect of the polysaccharide fractions of Trametes orientalis.
32895176	8	83	from	effect	1185:1190	arg1	metabolism					1257:1266	blood lipid metabolism	1245:1266	blood lipid metabolism	1245:1266	In a mouse model of hyperglycemic and hyperlipidemic, the glucose-lowering effect of TOPS-1 (0.1, 0.2, and 0.4 g/kg) and its effect on blood lipid metabolism were investigated in comparison with Xiaoke pills (5 pills/kg) and Danshen tablets (0.5 g/kg).
32895176	13	84	dep	polysaccharides	2145:2159	arg1	T.					2131:2132	T.	2131:2132	T.	2131:2132	CONCLUSIONS T. orientalis polysaccharides have anti-oxidation, glucose-lowering and lipid-lowering effects in mice, and their glucose-lowering effect is probably medicated by reducing oxidative stress and ameliorating lipid metabolism disorder.
32895176	8	85	theme	Danshen	1335:1341	arg1	g/kg					1356:1359	0.5 g/kg	1352:1359	0.5 g/kg	1352:1359	In a mouse model of hyperglycemic and hyperlipidemic, the glucose-lowering effect of TOPS-1 (0.1, 0.2, and 0.4 g/kg) and its effect on blood lipid metabolism were investigated in comparison with Xiaoke pills (5 pills/kg) and Danshen tablets (0.5 g/kg).
32895176	8	85	theme	Danshen	1335:1341	arg1	tablets					1343:1349	Danshen tablets	1335:1349	Danshen tablets (0.5 g/kg)	1335:1360	In a mouse model of hyperglycemic and hyperlipidemic, the glucose-lowering effect of TOPS-1 (0.1, 0.2, and 0.4 g/kg) and its effect on blood lipid metabolism were investigated in comparison with Xiaoke pills (5 pills/kg) and Danshen tablets (0.5 g/kg).
32895176	7	86	theme	adipocyte	998:1006	arg1	intake					1016:1021	adipocyte glucose intake	998:1021	adipocyte glucose intake	998:1021	The in vitro inhibitory activities of TOPW-1 and TOPS-1 against DPP4, adipocyte glucose intake and lipase activity were tested to preliminarily assess their glucose-lowering effect.
32895176	2	87	dep	METHODS	213:219	arg1	chromatographed					269:283	chromatographed	269:283	were chromatographed on DEAE cellulose column using H2O and 0.5 mol/L NaCl solution as the eluent and DEAE-water and DEAE-salt with high polysaccharide contents	264:423	METHODS The crude polysaccharides of T. orientalis were chromatographed on DEAE cellulose column using H2O and 0.5 mol/L NaCl solution as the eluent and DEAE-water and DEAE-salt with high polysaccharide contents were collected.
32895176	11	88	theme	low-density	1880:1890	arg1	cholesterol					1904:1914	low-density lipoprotein cholesterol	1880:1914	low-density lipoprotein cholesterol	1880:1914	In the mouse model of hyperglycemia and hyperlipidemia, treatment with TOPS-1 (0.2 g/kg) enhanced the activity of lipase and significantly reduced fasting glucose level and serum contents of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol without causing death in the mice.
32895176	0	89	theme	lowering	10:17	arg1	effect					19:24	[Glucose- lowering effect	0:24	[Glucose- lowering effect of Trametes orientalis polysaccharides in hyperglycemic and hyperlipidemic mice	0:104	[Glucose- lowering effect of Trametes orientalis polysaccharides in hyperglycemic and hyperlipidemic mice].
32895176	12	90	from	dose	2051:2054	arg1	significant					1998:2008	significant	1998:2008	significant	1998:2008	The glucose-lowering effects of TOPS-1 was not significant at the low (0.1 g/kg) or high (0.4 g/kg) dose, and a high dose tended to increase the mortality of the mice.
32895176	12	90	from	dose	2051:2054	arg1	effects					1972:1978	The glucose-lowering effects	1951:1978	The glucose-lowering effects of TOPS-1	1951:1988	The glucose-lowering effects of TOPS-1 was not significant at the low (0.1 g/kg) or high (0.4 g/kg) dose, and a high dose tended to increase the mortality of the mice.
32895176	3	91	dep	fractions	533:541	arg1	TOPW-1					543:548	TOPW-1	543:548	TOPW-1	543:548	The two fractions were separated using Sephadex G-100 gel column to obtain 4 polysaccharide fractions TOPW-1, TOPW-2, TOPS-1, and TOPS-2.
32895176	3	91	dep	fractions	533:541	arg1	fractions					533:541	4 polysaccharide fractions	516:541	4 polysaccharide fractions TOPW-1, TOPW-2, TOPS-1, and TOPS-2	516:576	The two fractions were separated using Sephadex G-100 gel column to obtain 4 polysaccharide fractions TOPW-1, TOPW-2, TOPS-1, and TOPS-2.
32895176	3	91	dep	fractions	533:541	arg1	TOPS-1					559:564	TOPS-1	559:564	TOPS-1	559:564	The two fractions were separated using Sephadex G-100 gel column to obtain 4 polysaccharide fractions TOPW-1, TOPW-2, TOPS-1, and TOPS-2.
32895176	3	91	dep	fractions	533:541	arg1	TOPS-2					571:576	TOPS-2	571:576	TOPS-2	571:576	The two fractions were separated using Sephadex G-100 gel column to obtain 4 polysaccharide fractions TOPW-1, TOPW-2, TOPS-1, and TOPS-2.
32895176	3	91	dep	fractions	533:541	arg1	TOPW-2					551:556	TOPW-2	551:556	TOPW-2	551:556	The two fractions were separated using Sephadex G-100 gel column to obtain 4 polysaccharide fractions TOPW-1, TOPW-2, TOPS-1, and TOPS-2.
32895176	11	92	theme	hyperlipidemia	1689:1702	arg1	model					1662:1666	the mouse model	1652:1666	the mouse model of hyperglycemia and hyperlipidemia	1652:1702	In the mouse model of hyperglycemia and hyperlipidemia, treatment with TOPS-1 (0.2 g/kg) enhanced the activity of lipase and significantly reduced fasting glucose level and serum contents of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol without causing death in the mice.
32895176	2	93	theme	high	396:399	arg1	contents					416:423	high polysaccharide contents	396:423	high polysaccharide contents	396:423	METHODS The crude polysaccharides of T. orientalis were chromatographed on DEAE cellulose column using H2O and 0.5 mol/L NaCl solution as the eluent and DEAE-water and DEAE-salt with high polysaccharide contents were collected.
32895176	7	94	theme	TOPS-1	977:982	arg1	activities					952:961	The in vitro inhibitory activities	928:961	The in vitro inhibitory activities of TOPW-1 and TOPS-1 against DPP4, adipocyte glucose intake and lipase activity	928:1041	The in vitro inhibitory activities of TOPW-1 and TOPS-1 against DPP4, adipocyte glucose intake and lipase activity were tested to preliminarily assess their glucose-lowering effect.
32895176	12	95	dep	high	2035:2038	arg1	g/kg					2045:2048	0.4 g/kg	2041:2048	0.4 g/kg	2041:2048	The glucose-lowering effects of TOPS-1 was not significant at the low (0.1 g/kg) or high (0.4 g/kg) dose, and a high dose tended to increase the mortality of the mice.
32895176	4	96	theme	anti-oxidation	583:596	arg1	activity					598:605	The anti-oxidation activity	579:605	The anti-oxidation activity of the polysaccharide fractions	579:637	The anti-oxidation activity of the polysaccharide fractions was investigated with ABTS method.
32895176	2	97	dep	eluent	355:360	arg1	the					351:353	the	351:353	the	351:353	METHODS The crude polysaccharides of T. orientalis were chromatographed on DEAE cellulose column using H2O and 0.5 mol/L NaCl solution as the eluent and DEAE-water and DEAE-salt with high polysaccharide contents were collected.
32895176	0	98	theme	orientalis	38:47	arg1	polysaccharides					49:63	Trametes orientalis polysaccharides	29:63	Trametes orientalis polysaccharides	29:63	[Glucose- lowering effect of Trametes orientalis polysaccharides in hyperglycemic and hyperlipidemic mice].
32895176	13	99	theme	lipid	2337:2341	arg1	disorder					2354:2361	lipid metabolism disorder	2337:2361	lipid metabolism disorder	2337:2361	CONCLUSIONS T. orientalis polysaccharides have anti-oxidation, glucose-lowering and lipid-lowering effects in mice, and their glucose-lowering effect is probably medicated by reducing oxidative stress and ameliorating lipid metabolism disorder.
32895176	11	100	theme	mouse	1656:1660	arg1	model					1662:1666	the mouse model	1652:1666	the mouse model of hyperglycemia and hyperlipidemia	1652:1702	In the mouse model of hyperglycemia and hyperlipidemia, treatment with TOPS-1 (0.2 g/kg) enhanced the activity of lipase and significantly reduced fasting glucose level and serum contents of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol without causing death in the mice.
32895176	3	101	theme	G-100	489:493	arg1	column					499:504	Sephadex G-100 gel column	480:504	Sephadex G-100 gel column	480:504	The two fractions were separated using Sephadex G-100 gel column to obtain 4 polysaccharide fractions TOPW-1, TOPW-2, TOPS-1, and TOPS-2.
32895176	9	102	theme	RESULTS	1363:1369	arg1	polysaccharide					1396:1409	a homogeneous polysaccharide	1382:1409	a homogeneous polysaccharide composed mainly of D-mannose, D-glucose, D-galactose, and D-fucose	1382:1476	RESULTS TOPW-1 was a homogeneous polysaccharide composed mainly of D-mannose, D-glucose, D-galactose, and D-fucose, with weak antioxidant and hypoglycemic effects.
32895176	9	102	theme	RESULTS	1363:1369	arg1	TOPW-1					1371:1376	RESULTS TOPW-1	1363:1376	RESULTS TOPW-1	1363:1376	RESULTS TOPW-1 was a homogeneous polysaccharide composed mainly of D-mannose, D-glucose, D-galactose, and D-fucose, with weak antioxidant and hypoglycemic effects.
32895176	2	103	with	eluent	355:360	arg1	contents					416:423	high polysaccharide contents	396:423	high polysaccharide contents	396:423	METHODS The crude polysaccharides of T. orientalis were chromatographed on DEAE cellulose column using H2O and 0.5 mol/L NaCl solution as the eluent and DEAE-water and DEAE-salt with high polysaccharide contents were collected.
32895176	3	104	theme	polysaccharide	518:531	arg1	TOPS-2					571:576	TOPS-2	571:576	TOPS-2	571:576	The two fractions were separated using Sephadex G-100 gel column to obtain 4 polysaccharide fractions TOPW-1, TOPW-2, TOPS-1, and TOPS-2.
32895176	3	104	theme	polysaccharide	518:531	arg1	TOPW-1					543:548	TOPW-1	543:548	TOPW-1	543:548	The two fractions were separated using Sephadex G-100 gel column to obtain 4 polysaccharide fractions TOPW-1, TOPW-2, TOPS-1, and TOPS-2.
32895176	3	104	theme	polysaccharide	518:531	arg1	TOPS-1					559:564	TOPS-1	559:564	TOPS-1	559:564	The two fractions were separated using Sephadex G-100 gel column to obtain 4 polysaccharide fractions TOPW-1, TOPW-2, TOPS-1, and TOPS-2.
32895176	3	104	theme	polysaccharide	518:531	arg1	fractions					533:541	4 polysaccharide fractions	516:541	4 polysaccharide fractions TOPW-1, TOPW-2, TOPS-1, and TOPS-2	516:576	The two fractions were separated using Sephadex G-100 gel column to obtain 4 polysaccharide fractions TOPW-1, TOPW-2, TOPS-1, and TOPS-2.
32895176	3	104	theme	polysaccharide	518:531	arg1	TOPW-2					551:556	TOPW-2	551:556	TOPW-2	551:556	The two fractions were separated using Sephadex G-100 gel column to obtain 4 polysaccharide fractions TOPW-1, TOPW-2, TOPS-1, and TOPS-2.
32895176	13	105	theme	orientalis	2134:2143	arg1	polysaccharides					2145:2159	orientalis polysaccharides	2134:2159	orientalis polysaccharides	2134:2159	CONCLUSIONS T. orientalis polysaccharides have anti-oxidation, glucose-lowering and lipid-lowering effects in mice, and their glucose-lowering effect is probably medicated by reducing oxidative stress and ameliorating lipid metabolism disorder.
32895176	2	106	with	DEAE-water	366:375	arg1	contents					416:423	high polysaccharide contents	396:423	high polysaccharide contents	396:423	METHODS The crude polysaccharides of T. orientalis were chromatographed on DEAE cellulose column using H2O and 0.5 mol/L NaCl solution as the eluent and DEAE-water and DEAE-salt with high polysaccharide contents were collected.
32895176	13	107	theme	oxidative	2303:2311	arg1	stress					2313:2318	oxidative stress	2303:2318	oxidative stress	2303:2318	CONCLUSIONS T. orientalis polysaccharides have anti-oxidation, glucose-lowering and lipid-lowering effects in mice, and their glucose-lowering effect is probably medicated by reducing oxidative stress and ameliorating lipid metabolism disorder.
32895176	9	108	with	polysaccharide	1396:1409	arg1	effects					1518:1524	weak antioxidant and hypoglycemic effects	1484:1524	weak antioxidant and hypoglycemic effects	1484:1524	RESULTS TOPW-1 was a homogeneous polysaccharide composed mainly of D-mannose, D-glucose, D-galactose, and D-fucose, with weak antioxidant and hypoglycemic effects.
32895176	7	109	theme	inhibitory	941:950	arg1	activities					952:961	The in vitro inhibitory activities	928:961	The in vitro inhibitory activities of TOPW-1 and TOPS-1 against DPP4, adipocyte glucose intake and lipase activity	928:1041	The in vitro inhibitory activities of TOPW-1 and TOPS-1 against DPP4, adipocyte glucose intake and lipase activity were tested to preliminarily assess their glucose-lowering effect.
32895176	9	110	theme	homogeneous	1384:1394	arg1	polysaccharide					1396:1409	a homogeneous polysaccharide	1382:1409	a homogeneous polysaccharide composed mainly of D-mannose, D-glucose, D-galactose, and D-fucose	1382:1476	RESULTS TOPW-1 was a homogeneous polysaccharide composed mainly of D-mannose, D-glucose, D-galactose, and D-fucose, with weak antioxidant and hypoglycemic effects.
32895176	9	110	theme	homogeneous	1384:1394	arg1	TOPW-1					1371:1376	RESULTS TOPW-1	1363:1376	RESULTS TOPW-1	1363:1376	RESULTS TOPW-1 was a homogeneous polysaccharide composed mainly of D-mannose, D-glucose, D-galactose, and D-fucose, with weak antioxidant and hypoglycemic effects.
32895176	8	111	theme	TOPS-1	1195:1200	arg1	effect					1235:1240	its effect	1231:1240	its effect on blood lipid metabolism	1231:1266	In a mouse model of hyperglycemic and hyperlipidemic, the glucose-lowering effect of TOPS-1 (0.1, 0.2, and 0.4 g/kg) and its effect on blood lipid metabolism were investigated in comparison with Xiaoke pills (5 pills/kg) and Danshen tablets (0.5 g/kg).
32895176	8	111	theme	TOPS-1	1195:1200	arg1	effect					1185:1190	the glucose-lowering effect	1164:1190	the glucose-lowering effect of TOPS-1 (0.1, 0.2, and 0.4 g/kg)	1164:1225	In a mouse model of hyperglycemic and hyperlipidemic, the glucose-lowering effect of TOPS-1 (0.1, 0.2, and 0.4 g/kg) and its effect on blood lipid metabolism were investigated in comparison with Xiaoke pills (5 pills/kg) and Danshen tablets (0.5 g/kg).
32895176	2	112	with	DEAE-salt	381:389	arg1	contents					416:423	high polysaccharide contents	396:423	high polysaccharide contents	396:423	METHODS The crude polysaccharides of T. orientalis were chromatographed on DEAE cellulose column using H2O and 0.5 mol/L NaCl solution as the eluent and DEAE-water and DEAE-salt with high polysaccharide contents were collected.
32895176	10	113	theme	single	1544:1549	arg1	polysaccharide					1551:1564	a single polysaccharide	1542:1564	a single polysaccharide	1542:1564	TOPS-1 was not a single polysaccharide and at the concentration of 500 μg/mL showed an high ABTS clearance rate (90.15%).
32895176	10	113	theme	single	1544:1549	arg1	TOPS-1					1527:1532	TOPS-1	1527:1532	TOPS-1	1527:1532	TOPS-1 was not a single polysaccharide and at the concentration of 500 μg/mL showed an high ABTS clearance rate (90.15%).
32895176	7	114	theme	in	932:933	arg1	activities					952:961	The in vitro inhibitory activities	928:961	The in vitro inhibitory activities of TOPW-1 and TOPS-1 against DPP4, adipocyte glucose intake and lipase activity	928:1041	The in vitro inhibitory activities of TOPW-1 and TOPS-1 against DPP4, adipocyte glucose intake and lipase activity were tested to preliminarily assess their glucose-lowering effect.
34535264	7	0	theme	low	1663:1665	arg1	N-glycans					1676:1684	low abundant N-glycans	1663:1684	low abundant N-glycans containing acetyl and sulphate modifications	1663:1729	Subsequent tandem mass spectrometry analysis revealed the identification of 212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications.
34535264	8	1	theme	pathological	2036:2047	arg1	states					2049:2054	the two pathological states	2028:2054	the two pathological states	2028:2054	The identifications obtained through this platform were then applied to N-glycans released from a set of stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states.
34535264	3	2	theme	Human	557:561	arg1	source					586:591	an attractive source	572:591	an attractive source for N-glycan based biomarker studies	572:628	Human serum is an attractive source for N-glycan based biomarker studies as circulatory proteins are representative of one's physiology, with many serum proteins containing N-glycosylation.
34535264	3	2	theme	Human	557:561	arg1	serum					563:567	Human serum	557:567	Human serum	557:567	Human serum is an attractive source for N-glycan based biomarker studies as circulatory proteins are representative of one's physiology, with many serum proteins containing N-glycosylation.
34535264	7	3	theme	structures	1642:1651	arg1	identification					1590:1603	the identification	1586:1603	the identification of 212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications	1586:1729	Subsequent tandem mass spectrometry analysis revealed the identification of 212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications.
34535264	6	4	theme	-4-methylmorpholinium	1399:1419	arg1	chloride					1421:1428	4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride	1363:1428	4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination	1363:1475	Performing these separations offline enables subsequent derivatization with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination and the identification of sialic acid linkage isomers.
34535264	6	4	theme	-4-methylmorpholinium	1399:1419	arg1	DMT-MM					1431:1436	DMT-MM	1431:1436	DMT-MM	1431:1436	Performing these separations offline enables subsequent derivatization with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination and the identification of sialic acid linkage isomers.
34535264	7	5	theme	partial	1625:1631	arg1	structures					1642:1651	212 complete and partial N-glycan structures	1608:1651	212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications	1608:1729	Subsequent tandem mass spectrometry analysis revealed the identification of 212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications.
34535264	7	5	theme	partial	1625:1631	arg1	N-glycans					1676:1684	low abundant N-glycans	1663:1684	low abundant N-glycans containing acetyl and sulphate modifications	1663:1729	Subsequent tandem mass spectrometry analysis revealed the identification of 212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications.
34535264	6	6	theme	4,6-Dimethoxy-1,3,5-triazin-2-yl	1366:1397	arg1	chloride					1421:1428	4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride	1363:1428	4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination	1363:1475	Performing these separations offline enables subsequent derivatization with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination and the identification of sialic acid linkage isomers.
34535264	6	6	theme	4,6-Dimethoxy-1,3,5-triazin-2-yl	1366:1397	arg1	DMT-MM					1431:1436	DMT-MM	1431:1436	DMT-MM	1431:1436	Performing these separations offline enables subsequent derivatization with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination and the identification of sialic acid linkage isomers.
34535264	3	7	theme	attractive	575:584	arg1	source					586:591	an attractive source	572:591	an attractive source for N-glycan based biomarker studies	572:628	Human serum is an attractive source for N-glycan based biomarker studies as circulatory proteins are representative of one's physiology, with many serum proteins containing N-glycosylation.
34535264	3	7	theme	attractive	575:584	arg1	serum					563:567	Human serum	557:567	Human serum	557:567	Human serum is an attractive source for N-glycan based biomarker studies as circulatory proteins are representative of one's physiology, with many serum proteins containing N-glycosylation.
34535264	1	8	theme	post-translational	211:228	arg1	modification					230:241	a prominent co- and post-translational modification	191:241	a prominent co- and post-translational modification which contributes to a variety of important biological functions	191:306	Glycosylation is a prominent co- and post-translational modification which contributes to a variety of important biological functions.
34535264	1	8	theme	post-translational	211:228	arg1	Glycosylation					174:186	Glycosylation	174:186	Glycosylation	174:186	Glycosylation is a prominent co- and post-translational modification which contributes to a variety of important biological functions.
34535264	7	9	theme	complete	1612:1619	arg1	structures					1642:1651	212 complete and partial N-glycan structures	1608:1651	212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications	1608:1729	Subsequent tandem mass spectrometry analysis revealed the identification of 212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications.
34535264	7	9	theme	complete	1612:1619	arg1	N-glycans					1676:1684	low abundant N-glycans	1663:1684	low abundant N-glycans containing acetyl and sulphate modifications	1663:1729	Subsequent tandem mass spectrometry analysis revealed the identification of 212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications.
34535264	7	10	theme	spectrometry	1555:1566	arg1	analysis					1568:1575	Subsequent tandem mass spectrometry analysis	1532:1575	Subsequent tandem mass spectrometry analysis	1532:1575	Subsequent tandem mass spectrometry analysis revealed the identification of 212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications.
34535264	7	11	contain	containing	1686:1695	arg2	modifications					1717:1729	sulphate modifications	1708:1729	sulphate modifications	1708:1729	Subsequent tandem mass spectrometry analysis revealed the identification of 212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications.
34535264	7	11	contain	containing	1686:1695	arg2	acetyl					1697:1702	acetyl	1697:1702	acetyl	1697:1702	Subsequent tandem mass spectrometry analysis revealed the identification of 212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications.
34535264	7	11	contain	containing	1686:1695	arg1	N-glycans					1676:1684	low abundant N-glycans	1663:1684	low abundant N-glycans containing acetyl and sulphate modifications	1663:1729	Subsequent tandem mass spectrometry analysis revealed the identification of 212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications.
34535264	7	12	theme	tandem	1543:1548	arg1	spectrometry					1555:1566	Subsequent tandem mass spectrometry	1532:1566	Subsequent tandem mass spectrometry analysis	1532:1575	Subsequent tandem mass spectrometry analysis revealed the identification of 212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications.
34535264	0	13	theme	N-glycome	146:154	arg1	characterization					156:171	comprehensive serum N-glycome characterization	126:171	comprehensive serum N-glycome characterization	126:171	2-Dimensional ultra-high performance liquid chromatography and DMT-MM derivatization paired with tandem mass spectrometry for comprehensive serum N-glycome characterization.
34535264	3	14	theme	based	606:610	arg1	studies					622:628	N-glycan based biomarker studies	597:628	N-glycan based biomarker studies	597:628	Human serum is an attractive source for N-glycan based biomarker studies as circulatory proteins are representative of one's physiology, with many serum proteins containing N-glycosylation.
34535264	6	15	theme	linkage	1455:1461	arg1	determination					1463:1475	sialic acid linkage determination	1443:1475	sialic acid linkage determination	1443:1475	Performing these separations offline enables subsequent derivatization with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination and the identification of sialic acid linkage isomers.
34535264	6	16	theme	subsequent	1332:1341	arg1	derivatization					1343:1356	subsequent derivatization	1332:1356	subsequent derivatization with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination	1332:1475	Performing these separations offline enables subsequent derivatization with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination and the identification of sialic acid linkage isomers.
34535264	6	17	theme	sialic	1443:1448	arg1	determination					1463:1475	sialic acid linkage determination	1443:1475	sialic acid linkage determination	1443:1475	Performing these separations offline enables subsequent derivatization with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination and the identification of sialic acid linkage isomers.
34535264	3	18	theme	circulatory	633:643	arg1	proteins					645:652	circulatory proteins	633:652	circulatory proteins	633:652	Human serum is an attractive source for N-glycan based biomarker studies as circulatory proteins are representative of one's physiology, with many serum proteins containing N-glycosylation.
34535264	4	19	theme	serum	800:804	arg1	N-glycome					806:814	the serum N-glycome	796:814	the serum N-glycome	796:814	The difficulty in comprehensively characterizing the serum N-glycome arises from the absence of a biosynthetic template resulting in great structural heterogeneity and complexity.
34535264	2	20	theme	glycosylation	317:329	arg1	N-glycosylation					361:375	N-glycosylation	361:375	particularly N-glycosylation	348:375	Protein glycosylation characteristics, particularly N-glycosylation, are influenced by changes in one's pathological state, such as through the presence of disease, and as such, there is great interest in N-glycans as potential disease biomarkers.
34535264	2	20	theme	glycosylation	317:329	arg1	characteristics					331:345	Protein glycosylation characteristics	309:345	Protein glycosylation characteristics	309:345	Protein glycosylation characteristics, particularly N-glycosylation, are influenced by changes in one's pathological state, such as through the presence of disease, and as such, there is great interest in N-glycans as potential disease biomarkers.
34535264	5	21	theme	offline	1036:1042	arg1	chromatography					1070:1083	offline weak anion exchange (WAX) chromatography	1036:1083	offline weak anion exchange (WAX) chromatography in the first dimension	1036:1106	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	5	22	theme	2-dimensional	976:988	arg1	platform					1012:1019	a 2-dimensional liquid chromatography platform	974:1019	a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively	974:1284	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	6	23	with	derivatization	1343:1356	arg1	chloride					1421:1428	4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride	1363:1428	4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination	1363:1475	Performing these separations offline enables subsequent derivatization with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination and the identification of sialic acid linkage isomers.
34535264	6	23	with	derivatization	1343:1356	arg1	DMT-MM					1431:1436	DMT-MM	1431:1436	DMT-MM	1431:1436	Performing these separations offline enables subsequent derivatization with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination and the identification of sialic acid linkage isomers.
34535264	2	24	theme	potential	527:535	arg1	biomarkers					545:554	potential disease biomarkers	527:554	potential disease biomarkers	527:554	Protein glycosylation characteristics, particularly N-glycosylation, are influenced by changes in one's pathological state, such as through the presence of disease, and as such, there is great interest in N-glycans as potential disease biomarkers.
34535264	0	25	theme	tandem	97:102	arg1	spectrometry					109:120	tandem mass spectrometry	97:120	tandem mass spectrometry for comprehensive serum N-glycome characterization	97:171	2-Dimensional ultra-high performance liquid chromatography and DMT-MM derivatization paired with tandem mass spectrometry for comprehensive serum N-glycome characterization.
34535264	1	26	theme	co-	203:205	arg1	modification					230:241	a prominent co- and post-translational modification	191:241	a prominent co- and post-translational modification which contributes to a variety of important biological functions	191:306	Glycosylation is a prominent co- and post-translational modification which contributes to a variety of important biological functions.
34535264	1	26	theme	co-	203:205	arg1	Glycosylation					174:186	Glycosylation	174:186	Glycosylation	174:186	Glycosylation is a prominent co- and post-translational modification which contributes to a variety of important biological functions.
34535264	2	27	from	changes	396:402	arg1	state					426:430	one's pathological state	407:430	one's pathological state	407:430	Protein glycosylation characteristics, particularly N-glycosylation, are influenced by changes in one's pathological state, such as through the presence of disease, and as such, there is great interest in N-glycans as potential disease biomarkers.
34535264	6	28	theme	sialic	1503:1508	arg1	acid					1510:1513	sialic acid	1503:1513	sialic acid	1503:1513	Performing these separations offline enables subsequent derivatization with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination and the identification of sialic acid linkage isomers.
34535264	5	29	theme	separate	1193:1200	arg1	N-glycans					1202:1210	separate N-glycans	1193:1210	separate N-glycans	1193:1210	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	4	30	theme	biosynthetic	845:856	arg1	template					858:865	a biosynthetic template	843:865	a biosynthetic template resulting in great structural heterogeneity and complexity	843:924	The difficulty in comprehensively characterizing the serum N-glycome arises from the absence of a biosynthetic template resulting in great structural heterogeneity and complexity.
34535264	0	31	theme	comprehensive	126:138	arg1	characterization					156:171	comprehensive serum N-glycome characterization	126:171	comprehensive serum N-glycome characterization	126:171	2-Dimensional ultra-high performance liquid chromatography and DMT-MM derivatization paired with tandem mass spectrometry for comprehensive serum N-glycome characterization.
34535264	0	32	theme	2-Dimensional	0:12	arg1	chromatography					44:57	2-Dimensional ultra-high performance liquid chromatography	0:57	2-Dimensional ultra-high performance liquid chromatography	0:57	2-Dimensional ultra-high performance liquid chromatography and DMT-MM derivatization paired with tandem mass spectrometry for comprehensive serum N-glycome characterization.
34535264	5	33	theme	size	1267:1270	arg1	degree					1240:1245	degree	1240:1245	degree of sialylation, and size, respectively	1240:1284	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	5	34	theme	anion	1049:1053	arg1	chromatography					1070:1083	offline weak anion exchange (WAX) chromatography	1036:1083	offline weak anion exchange (WAX) chromatography in the first dimension	1036:1106	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	0	35	theme	performance	25:35	arg1	chromatography					44:57	2-Dimensional ultra-high performance liquid chromatography	0:57	2-Dimensional ultra-high performance liquid chromatography	0:57	2-Dimensional ultra-high performance liquid chromatography and DMT-MM derivatization paired with tandem mass spectrometry for comprehensive serum N-glycome characterization.
34535264	3	36	theme	many	699:702	arg1	proteins					710:717	many serum proteins	699:717	many serum proteins containing N-glycosylation	699:744	Human serum is an attractive source for N-glycan based biomarker studies as circulatory proteins are representative of one's physiology, with many serum proteins containing N-glycosylation.
34535264	5	37	theme	interaction	1124:1134	arg1	HILIC					1159:1163	HILIC	1159:1163	HILIC	1159:1163	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	5	37	theme	interaction	1124:1134	arg1	chromatography					1143:1156	hydrophilic interaction liquid chromatography	1112:1156	hydrophilic interaction liquid chromatography (HILIC)	1112:1164	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	2	38	theme	pathological	413:424	arg1	state					426:430	one's pathological state	407:430	one's pathological state	407:430	Protein glycosylation characteristics, particularly N-glycosylation, are influenced by changes in one's pathological state, such as through the presence of disease, and as such, there is great interest in N-glycans as potential disease biomarkers.
34535264	0	39	theme	DMT-MM	63:68	arg1	derivatization					70:83	DMT-MM derivatization	63:83	DMT-MM derivatization	63:83	2-Dimensional ultra-high performance liquid chromatography and DMT-MM derivatization paired with tandem mass spectrometry for comprehensive serum N-glycome characterization.
34535264	4	40	theme	structural	886:895	arg1	heterogeneity					897:909	great structural heterogeneity	880:909	great structural heterogeneity	880:909	The difficulty in comprehensively characterizing the serum N-glycome arises from the absence of a biosynthetic template resulting in great structural heterogeneity and complexity.
34535264	8	41	theme	stage	1837:1841	arg1	samples					1866:1872	stage 3 gastric cancer serum samples	1837:1872	stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states	1837:2054	The identifications obtained through this platform were then applied to N-glycans released from a set of stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states.
34535264	7	42	theme	abundant	1667:1674	arg1	N-glycans					1676:1684	low abundant N-glycans	1663:1684	low abundant N-glycans containing acetyl and sulphate modifications	1663:1729	Subsequent tandem mass spectrometry analysis revealed the identification of 212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications.
34535264	5	43	theme	first	1092:1096	arg1	dimension					1098:1106	the first dimension	1088:1106	the first dimension	1088:1106	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	4	44	theme	great	880:884	arg1	heterogeneity					897:909	great structural heterogeneity	880:909	great structural heterogeneity	880:909	The difficulty in comprehensively characterizing the serum N-glycome arises from the absence of a biosynthetic template resulting in great structural heterogeneity and complexity.
34535264	8	45	theme	gastric	1845:1851	arg1	samples					1866:1872	stage 3 gastric cancer serum samples	1837:1872	stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states	1837:2054	The identifications obtained through this platform were then applied to N-glycans released from a set of stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states.
34535264	1	46	theme	biological	287:296	arg1	functions					298:306	important biological functions	277:306	important biological functions	277:306	Glycosylation is a prominent co- and post-translational modification which contributes to a variety of important biological functions.
34535264	8	47	theme	serum	1860:1864	arg1	samples					1866:1872	stage 3 gastric cancer serum samples	1837:1872	stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states	1837:2054	The identifications obtained through this platform were then applied to N-glycans released from a set of stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states.
34535264	7	48	theme	N-glycan	1633:1640	arg1	structures					1642:1651	212 complete and partial N-glycan structures	1608:1651	212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications	1608:1729	Subsequent tandem mass spectrometry analysis revealed the identification of 212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications.
34535264	7	48	theme	N-glycan	1633:1640	arg1	N-glycans					1676:1684	low abundant N-glycans	1663:1684	low abundant N-glycans containing acetyl and sulphate modifications	1663:1729	Subsequent tandem mass spectrometry analysis revealed the identification of 212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications.
34535264	5	49	theme	liquid	990:995	arg1	platform					1012:1019	a 2-dimensional liquid chromatography platform	974:1019	a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively	974:1284	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	0	50	with	paired	85:90	arg1	spectrometry					109:120	tandem mass spectrometry	97:120	tandem mass spectrometry for comprehensive serum N-glycome characterization	97:171	2-Dimensional ultra-high performance liquid chromatography and DMT-MM derivatization paired with tandem mass spectrometry for comprehensive serum N-glycome characterization.
34535264	6	51	theme	linkage	1515:1521	arg1	isomers					1523:1529	linkage isomers	1515:1529	linkage isomers	1515:1529	Performing these separations offline enables subsequent derivatization with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination and the identification of sialic acid linkage isomers.
34535264	3	52	theme	biomarker	612:620	arg1	studies					622:628	N-glycan based biomarker studies	597:628	N-glycan based biomarker studies	597:628	Human serum is an attractive source for N-glycan based biomarker studies as circulatory proteins are representative of one's physiology, with many serum proteins containing N-glycosylation.
34535264	3	53	contain	containing	719:728	arg2	N-glycosylation					730:744	N-glycosylation	730:744	N-glycosylation	730:744	Human serum is an attractive source for N-glycan based biomarker studies as circulatory proteins are representative of one's physiology, with many serum proteins containing N-glycosylation.
34535264	3	53	contain	containing	719:728	arg1	proteins					710:717	many serum proteins	699:717	many serum proteins containing N-glycosylation	699:744	Human serum is an attractive source for N-glycan based biomarker studies as circulatory proteins are representative of one's physiology, with many serum proteins containing N-glycosylation.
34535264	3	54	theme	N-glycan	597:604	arg1	studies					622:628	N-glycan based biomarker studies	597:628	N-glycan based biomarker studies	597:628	Human serum is an attractive source for N-glycan based biomarker studies as circulatory proteins are representative of one's physiology, with many serum proteins containing N-glycosylation.
34535264	5	55	from	chromatography	1143:1156	arg1	dimension					1098:1106	the first dimension	1088:1106	the first dimension	1088:1106	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	5	56	theme	chromatography	997:1010	arg1	platform					1012:1019	a 2-dimensional liquid chromatography platform	974:1019	a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively	974:1284	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	5	57	theme	second	1173:1178	arg1	dimension					1180:1188	the second dimension	1169:1188	the second dimension to separate N-glycans by charge	1169:1220	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	7	58	theme	Subsequent	1532:1541	arg1	spectrometry					1555:1566	Subsequent tandem mass spectrometry	1532:1566	Subsequent tandem mass spectrometry analysis	1532:1575	Subsequent tandem mass spectrometry analysis revealed the identification of 212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications.
34535264	6	59	theme	acid	1450:1453	arg1	determination					1463:1475	sialic acid linkage determination	1443:1475	sialic acid linkage determination	1443:1475	Performing these separations offline enables subsequent derivatization with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination and the identification of sialic acid linkage isomers.
34535264	8	60	theme	post-op	1924:1930	arg1	resection					1940:1948	(post-op) tumour resection	1923:1948	(post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states	1923:2054	The identifications obtained through this platform were then applied to N-glycans released from a set of stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states.
34535264	8	61	theme	tumour	1933:1938	arg1	resection					1940:1948	(post-op) tumour resection	1923:1948	(post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states	1923:2054	The identifications obtained through this platform were then applied to N-glycans released from a set of stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states.
34535264	3	62	with	representative	658:671	arg1	proteins					710:717	many serum proteins	699:717	many serum proteins containing N-glycosylation	699:744	Human serum is an attractive source for N-glycan based biomarker studies as circulatory proteins are representative of one's physiology, with many serum proteins containing N-glycosylation.
34535264	2	63	theme	Protein	309:315	arg1	N-glycosylation					361:375	N-glycosylation	361:375	particularly N-glycosylation	348:375	Protein glycosylation characteristics, particularly N-glycosylation, are influenced by changes in one's pathological state, such as through the presence of disease, and as such, there is great interest in N-glycans as potential disease biomarkers.
34535264	2	63	theme	Protein	309:315	arg1	characteristics					331:345	Protein glycosylation characteristics	309:345	Protein glycosylation characteristics	309:345	Protein glycosylation characteristics, particularly N-glycosylation, are influenced by changes in one's pathological state, such as through the presence of disease, and as such, there is great interest in N-glycans as potential disease biomarkers.
34535264	6	64	with	identification	1485:1498	arg1	chloride					1421:1428	4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride	1363:1428	4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination	1363:1475	Performing these separations offline enables subsequent derivatization with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination and the identification of sialic acid linkage isomers.
34535264	6	64	with	identification	1485:1498	arg1	DMT-MM					1431:1436	DMT-MM	1431:1436	DMT-MM	1431:1436	Performing these separations offline enables subsequent derivatization with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination and the identification of sialic acid linkage isomers.
34535264	2	65	theme	disease	537:543	arg1	biomarkers					545:554	potential disease biomarkers	527:554	potential disease biomarkers	527:554	Protein glycosylation characteristics, particularly N-glycosylation, are influenced by changes in one's pathological state, such as through the presence of disease, and as such, there is great interest in N-glycans as potential disease biomarkers.
34535264	6	66	dep	enables	1324:1330	arg1	Performing					1287:1296	Performing	1287:1296	Performing these separations	1287:1314	Performing these separations offline enables subsequent derivatization with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination and the identification of sialic acid linkage isomers.
34535264	0	67	theme	mass	104:107	arg1	spectrometry					109:120	tandem mass spectrometry	97:120	tandem mass spectrometry for comprehensive serum N-glycome characterization	97:171	2-Dimensional ultra-high performance liquid chromatography and DMT-MM derivatization paired with tandem mass spectrometry for comprehensive serum N-glycome characterization.
34535264	1	68	theme	prominent	193:201	arg1	modification					230:241	a prominent co- and post-translational modification	191:241	a prominent co- and post-translational modification which contributes to a variety of important biological functions	191:306	Glycosylation is a prominent co- and post-translational modification which contributes to a variety of important biological functions.
34535264	1	68	theme	prominent	193:201	arg1	Glycosylation					174:186	Glycosylation	174:186	Glycosylation	174:186	Glycosylation is a prominent co- and post-translational modification which contributes to a variety of important biological functions.
34535264	6	69	dep	derivatization	1343:1356	arg1	isomers					1523:1529	linkage isomers	1515:1529	linkage isomers	1515:1529	Performing these separations offline enables subsequent derivatization with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination and the identification of sialic acid linkage isomers.
34535264	6	70	theme	acid	1510:1513	arg1	identification					1485:1498	the identification	1481:1498	the identification of sialic acid	1481:1513	Performing these separations offline enables subsequent derivatization with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination and the identification of sialic acid linkage isomers.
34535264	6	70	theme	acid	1510:1513	arg1	derivatization					1343:1356	subsequent derivatization	1332:1356	subsequent derivatization with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination	1332:1475	Performing these separations offline enables subsequent derivatization with 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) for sialic acid linkage determination and the identification of sialic acid linkage isomers.
34535264	8	71	theme	serum	1973:1977	arg1	N-glycome					1979:1987	the serum N-glycome	1969:1987	the serum N-glycome	1969:1987	The identifications obtained through this platform were then applied to N-glycans released from a set of stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states.
34535264	5	72	theme	WAX	1065:1067	arg1	chromatography					1070:1083	offline weak anion exchange (WAX) chromatography	1036:1083	offline weak anion exchange (WAX) chromatography in the first dimension	1036:1106	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	7	73	theme	mass	1550:1553	arg1	spectrometry					1555:1566	Subsequent tandem mass spectrometry	1532:1566	Subsequent tandem mass spectrometry analysis	1532:1575	Subsequent tandem mass spectrometry analysis revealed the identification of 212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications.
34535264	2	74	from	interest	502:509	arg1	N-glycans					514:522	N-glycans	514:522	N-glycans as potential disease biomarkers	514:554	Protein glycosylation characteristics, particularly N-glycosylation, are influenced by changes in one's pathological state, such as through the presence of disease, and as such, there is great interest in N-glycans as potential disease biomarkers.
34535264	0	75	theme	serum	140:144	arg1	characterization					156:171	comprehensive serum N-glycome characterization	126:171	comprehensive serum N-glycome characterization	126:171	2-Dimensional ultra-high performance liquid chromatography and DMT-MM derivatization paired with tandem mass spectrometry for comprehensive serum N-glycome characterization.
34535264	8	76	attach	released	1814:1821	arg1	samples					1866:1872	stage 3 gastric cancer serum samples	1837:1872	stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states	1837:2054	The identifications obtained through this platform were then applied to N-glycans released from a set of stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states.
34535264	8	76	attach	released	1814:1821	arg1	set					1830:1832	a set	1828:1832	a set of stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states	1828:2054	The identifications obtained through this platform were then applied to N-glycans released from a set of stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states.
34535264	8	76	attach	released	1814:1821	arg2	N-glycans					1804:1812	N-glycans	1804:1812	N-glycans released from a set of stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states	1804:2054	The identifications obtained through this platform were then applied to N-glycans released from a set of stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states.
34535264	2	77	dep	such	433:436	arg1	as					438:439	as	438:439	as	438:439	Protein glycosylation characteristics, particularly N-glycosylation, are influenced by changes in one's pathological state, such as through the presence of disease, and as such, there is great interest in N-glycans as potential disease biomarkers.
34535264	0	78	theme	ultra-high	14:23	arg1	chromatography					44:57	2-Dimensional ultra-high performance liquid chromatography	0:57	2-Dimensional ultra-high performance liquid chromatography	0:57	2-Dimensional ultra-high performance liquid chromatography and DMT-MM derivatization paired with tandem mass spectrometry for comprehensive serum N-glycome characterization.
34535264	5	79	theme	weak	1044:1047	arg1	chromatography					1070:1083	offline weak anion exchange (WAX) chromatography	1036:1083	offline weak anion exchange (WAX) chromatography in the first dimension	1036:1106	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	0	80	theme	liquid	37:42	arg1	chromatography					44:57	2-Dimensional ultra-high performance liquid chromatography	0:57	2-Dimensional ultra-high performance liquid chromatography	0:57	2-Dimensional ultra-high performance liquid chromatography and DMT-MM derivatization paired with tandem mass spectrometry for comprehensive serum N-glycome characterization.
34535264	5	81	theme	exchange	1055:1062	arg1	chromatography					1070:1083	offline weak anion exchange (WAX) chromatography	1036:1083	offline weak anion exchange (WAX) chromatography in the first dimension	1036:1106	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	5	82	theme	hydrophilic	1112:1122	arg1	HILIC					1159:1163	HILIC	1159:1163	HILIC	1159:1163	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	5	82	theme	hydrophilic	1112:1122	arg1	chromatography					1143:1156	hydrophilic interaction liquid chromatography	1112:1156	hydrophilic interaction liquid chromatography (HILIC)	1112:1164	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	3	83	theme	physiology	682:691	arg1	representative					658:671	representative	658:671	representative	658:671	Human serum is an attractive source for N-glycan based biomarker studies as circulatory proteins are representative of one's physiology, with many serum proteins containing N-glycosylation.
34535264	5	84	dep	developed	964:972	arg1	help					930:933	help	930:933	To help overcome these challenges	927:959	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	2	85	theme	disease	465:471	arg1	presence					453:460	the presence	449:460	the presence of disease	449:471	Protein glycosylation characteristics, particularly N-glycosylation, are influenced by changes in one's pathological state, such as through the presence of disease, and as such, there is great interest in N-glycans as potential disease biomarkers.
34535264	5	86	theme	sialylation	1250:1260	arg1	degree					1240:1245	degree	1240:1245	degree of sialylation, and size, respectively	1240:1284	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	7	87	theme	sulphate	1708:1715	arg1	modifications					1717:1729	sulphate modifications	1708:1729	sulphate modifications	1708:1729	Subsequent tandem mass spectrometry analysis revealed the identification of 212 complete and partial N-glycan structures including low abundant N-glycans containing acetyl and sulphate modifications.
34535264	5	88	theme	liquid	1136:1141	arg1	HILIC					1159:1163	HILIC	1159:1163	HILIC	1159:1163	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	5	88	theme	liquid	1136:1141	arg1	chromatography					1143:1156	hydrophilic interaction liquid chromatography	1112:1156	hydrophilic interaction liquid chromatography (HILIC)	1112:1164	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	2	89	theme	great	496:500	arg1	interest					502:509	great interest	496:509	great interest in N-glycans as potential disease biomarkers	496:554	Protein glycosylation characteristics, particularly N-glycosylation, are influenced by changes in one's pathological state, such as through the presence of disease, and as such, there is great interest in N-glycans as potential disease biomarkers.
34535264	3	90	theme	serum	704:708	arg1	proteins					710:717	many serum proteins	699:717	many serum proteins containing N-glycosylation	699:744	Human serum is an attractive source for N-glycan based biomarker studies as circulatory proteins are representative of one's physiology, with many serum proteins containing N-glycosylation.
34535264	4	91	theme	template	858:865	arg1	absence					832:838	the absence	828:838	the absence of a biosynthetic template resulting in great structural heterogeneity and complexity	828:924	The difficulty in comprehensively characterizing the serum N-glycome arises from the absence of a biosynthetic template resulting in great structural heterogeneity and complexity.
34535264	8	92	theme	cancer	1853:1858	arg1	samples					1866:1872	stage 3 gastric cancer serum samples	1837:1872	stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states	1837:2054	The identifications obtained through this platform were then applied to N-glycans released from a set of stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states.
34535264	1	93	theme	important	277:285	arg1	functions					298:306	important biological functions	277:306	important biological functions	277:306	Glycosylation is a prominent co- and post-translational modification which contributes to a variety of important biological functions.
34535264	8	94	theme	samples	1866:1872	arg1	samples					1866:1872	stage 3 gastric cancer serum samples	1837:1872	stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states	1837:2054	The identifications obtained through this platform were then applied to N-glycans released from a set of stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states.
34535264	8	94	theme	samples	1866:1872	arg1	set					1830:1832	a set	1828:1832	a set of stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states	1828:2054	The identifications obtained through this platform were then applied to N-glycans released from a set of stage 3 gastric cancer serum samples obtained from patients before (pre-op) and after (post-op) tumour resection to investigate how the serum N-glycome can facilitate differentiation between the two pathological states.
34535264	5	95	from	chromatography	1070:1083	arg1	dimension					1098:1106	the first dimension	1088:1106	the first dimension	1088:1106	To help overcome these challenges we developed a 2-dimensional liquid chromatography platform which utilizes offline weak anion exchange (WAX) chromatography in the first dimension and hydrophilic interaction liquid chromatography (HILIC) in the second dimension to separate N-glycans by charge, corresponding to degree of sialylation, and size, respectively.
34535264	1	96	theme	functions	298:306	arg1	functions					298:306	important biological functions	277:306	important biological functions	277:306	Glycosylation is a prominent co- and post-translational modification which contributes to a variety of important biological functions.
34535264	1	96	theme	functions	298:306	arg1	variety					266:272	a variety	264:272	a variety of important biological functions	264:306	Glycosylation is a prominent co- and post-translational modification which contributes to a variety of important biological functions.
34974152	3	0	theme	gelatin	722:728	arg1	hydrogels					744:752	xanthan, gelatin, and glycerol hydrogels	713:752	xanthan, gelatin, and glycerol hydrogels	713:752	According to their weight ratios, xanthan, gelatin, and glycerol hydrogels are named.
34974152	7	1	theme	4523 ± 133 g m-2	1159:1174	arg1	d-1					1176:1178	4523 ± 133 g m-2 d-1	1159:1178	4523 ± 133 g m-2 d-1	1159:1178	Water vapor transmission of XGHs and KXGHs was between 3059.09 ± 126 and 4523 ± 133 g m-2 d-1; therefore, they can be suitable for granulating, low to moderate exudate wounds.
34974152	0	2	theme	C.	90:91	arg1	delivery					70:77	local delivery	64:77	local delivery of Vitamin C.	64:91	Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of Vitamin C. Recently, functional dressings that can protect the wound area from dehydration and bacterial infection and support healing have gained importance in place of passive dressings.
34974152	0	3	theme	bacterial	177:185	arg1	infection					187:195	bacterial infection	177:195	bacterial infection	177:195	Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of Vitamin C. Recently, functional dressings that can protect the wound area from dehydration and bacterial infection and support healing have gained importance in place of passive dressings.
34974152	6	4	theme	fibroblasts	1033:1043	arg1	viability					1045:1053	L929 fibroblasts viability	1028:1053	L929 fibroblasts viability	1028:1053	The addition of keratin to X1:GEL1:GLY2 improved L929 fibroblasts viability and increased protein release.
34974152	0	5	theme	functional	103:112	arg1	dressings					114:122	functional dressings	103:122	functional dressings that can protect the wound area from dehydration and bacterial infection and support healing	103:215	Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of Vitamin C. Recently, functional dressings that can protect the wound area from dehydration and bacterial infection and support healing have gained importance in place of passive dressings.
34974152	1	6	theme	keratin/xanthan/gelatin	352:374	arg1	KXGHs					387:391	KXGHs	387:391	KXGHs	387:391	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	1	6	theme	keratin/xanthan/gelatin	352:374	arg1	hydrogels					376:384	temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels	299:384	temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC)	299:515	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	2	7	theme	glycerol	598:605	arg1	concentrations					607:620	different glycerol concentrations	588:620	different glycerol concentrations	588:620	Firstly, xanthan/gelatin hydrogels were produced by crosslinking with different glycerol concentrations and characterized to determine the hydrogel composition.
34974152	1	8	theme	wound	452:456	arg1	dressing					458:465	a wound dressing	450:465	a wound dressing that can provide local delivery of Vitamin C (VC)	450:515	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	1	8	theme	wound	452:456	arg1	applicability					433:445	applicability	433:445	applicability	433:445	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	1	8	theme	wound	452:456	arg1	capacity					420:427	high absorption capacity	404:427	high absorption capacity	404:427	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	4	9	theme	weight	774:779	arg1	ratio					781:785	their weight ratio	768:785	their weight ratio	768:785	If their weight ratio is 1:1:2 (w/w/w), the group name is selected as X1:GEL1:GLY2.
34974152	9	10	theme	fibroblasts	1463:1473	arg1	synthesis					1445:1453	the collagen synthesis	1432:1453	the collagen synthesis of L929 fibroblasts	1432:1473	VC was released for 100 h, and VC containing XGHs and KXGHs increased the collagen synthesis of L929 fibroblasts.
34974152	7	11	theme	KXGHs	1123:1127	arg1	transmission					1098:1109	Water vapor transmission	1086:1109	Water vapor transmission of XGHs and KXGHs	1086:1127	Water vapor transmission of XGHs and KXGHs was between 3059.09 ± 126 and 4523 ± 133 g m-2 d-1; therefore, they can be suitable for granulating, low to moderate exudate wounds.
34974152	3	12	theme	glycerol	735:742	arg1	hydrogels					744:752	xanthan, gelatin, and glycerol hydrogels	713:752	xanthan, gelatin, and glycerol hydrogels	713:752	According to their weight ratios, xanthan, gelatin, and glycerol hydrogels are named.
34974152	2	13	theme	different	588:596	arg1	concentrations					607:620	different glycerol concentrations	588:620	different glycerol concentrations	588:620	Firstly, xanthan/gelatin hydrogels were produced by crosslinking with different glycerol concentrations and characterized to determine the hydrogel composition.
34974152	0	14	theme	passive	252:258	arg1	dressings					260:268	passive dressings	252:268	passive dressings	252:268	Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of Vitamin C. Recently, functional dressings that can protect the wound area from dehydration and bacterial infection and support healing have gained importance in place of passive dressings.
34974152	2	15	theme	hydrogel	657:664	arg1	composition					666:676	the hydrogel composition	653:676	the hydrogel composition	653:676	Firstly, xanthan/gelatin hydrogels were produced by crosslinking with different glycerol concentrations and characterized to determine the hydrogel composition.
34974152	6	16	dep	addition	983:990	arg1	GLY2					1014:1017	GLY2	1014:1017	The addition of keratin to X1:GEL1:GLY2	979:1017	The addition of keratin to X1:GEL1:GLY2 improved L929 fibroblasts viability and increased protein release.
34974152	6	16	dep	addition	983:990	arg1	GEL1					1009:1012	GEL1	1009:1012	The addition of keratin to X1:GEL1:GLY2	979:1017	The addition of keratin to X1:GEL1:GLY2 improved L929 fibroblasts viability and increased protein release.
34974152	6	17	theme	keratin	995:1001	arg1	addition					983:990	The addition	979:990	The addition of keratin to X1:GEL1:GLY2	979:1017	The addition of keratin to X1:GEL1:GLY2 improved L929 fibroblasts viability and increased protein release.
34974152	7	18	theme	XGHs	1114:1117	arg1	transmission					1098:1109	Water vapor transmission	1086:1109	Water vapor transmission of XGHs and KXGHs	1086:1127	Water vapor transmission of XGHs and KXGHs was between 3059.09 ± 126 and 4523 ± 133 g m-2 d-1; therefore, they can be suitable for granulating, low to moderate exudate wounds.
34974152	5	19	theme	water	927:931	arg1	rate					952:955	water vapor transmission rate	927:955	water vapor transmission rate (WVTR)	927:962	X1:GEL1:GLY2 hydrogel was selected for biocompatibility, mechanical property, water vapor transmission rate (WVTR), and porosity.
34974152	5	19	theme	water	927:931	arg1	WVTR					958:961	WVTR	958:961	WVTR	958:961	X1:GEL1:GLY2 hydrogel was selected for biocompatibility, mechanical property, water vapor transmission rate (WVTR), and porosity.
34974152	7	20	theme	exudate	1246:1252	arg1	wounds					1254:1259	granulating, low to moderate exudate wounds	1217:1259	granulating, low to moderate exudate wounds	1217:1259	Water vapor transmission of XGHs and KXGHs was between 3059.09 ± 126 and 4523 ± 133 g m-2 d-1; therefore, they can be suitable for granulating, low to moderate exudate wounds.
34974152	4	21	dep	X1	835:836	arg1	GEL1					838:841	GEL1	838:841	X1:GEL1:GLY2	835:846	If their weight ratio is 1:1:2 (w/w/w), the group name is selected as X1:GEL1:GLY2.
34974152	4	21	dep	X1	835:836	arg1	GLY2					843:846	GLY2	843:846	X1:GEL1:GLY2	835:846	If their weight ratio is 1:1:2 (w/w/w), the group name is selected as X1:GEL1:GLY2.
34974152	11	22	theme	wound	1651:1655	arg1	materials					1666:1674	temporary wound dressing materials	1641:1674	temporary wound dressing materials	1641:1674	In conclusion, our results suggest that VC-XGH and VC-KXGH can be candidates for temporary wound dressing materials for skin wounds.
34974152	1	23	contain	have	399:402	arg2	applicability					433:445	applicability	433:445	applicability	433:445	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	1	23	contain	have	399:402	arg1	KXGHs					387:391	KXGHs	387:391	KXGHs	387:391	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	1	23	contain	have	399:402	arg2	capacity					420:427	high absorption capacity	404:427	high absorption capacity	404:427	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	1	23	contain	have	399:402	arg1	hydrogels					376:384	temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels	299:384	temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC)	299:515	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	1	23	contain	have	399:402	arg2	dressing					458:465	a wound dressing	450:465	a wound dressing that can provide local delivery of Vitamin C (VC)	450:515	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	0	24	theme	Xanthan-gelatin	0:14	arg1	dressings					50:58	Xanthan-gelatin and xanthan-gelatin-keratin wound dressings	0:58	Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of Vitamin C.	0:91	Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of Vitamin C. Recently, functional dressings that can protect the wound area from dehydration and bacterial infection and support healing have gained importance in place of passive dressings.
34974152	11	25	theme	dressing	1657:1664	arg1	materials					1666:1674	temporary wound dressing materials	1641:1674	temporary wound dressing materials	1641:1674	In conclusion, our results suggest that VC-XGH and VC-KXGH can be candidates for temporary wound dressing materials for skin wounds.
34974152	1	26	theme	local	484:488	arg1	delivery					490:497	local delivery	484:497	local delivery of Vitamin C (VC)	484:515	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	6	27	theme	protein	1069:1075	arg1	release					1077:1083	protein release	1069:1083	protein release	1069:1083	The addition of keratin to X1:GEL1:GLY2 improved L929 fibroblasts viability and increased protein release.
34974152	10	28	dep	hydrogels	1487:1495	arg1	KXGH					1503:1506	KXGH	1503:1506	KXGH	1503:1506	All of the hydrogels (XGH, KXGH, and VC-KXGHs) inhibited the bacteria transmission.
34974152	10	28	dep	hydrogels	1487:1495	arg1	VC-KXGHs					1513:1520	VC-KXGHs	1513:1520	VC-KXGHs	1513:1520	All of the hydrogels (XGH, KXGH, and VC-KXGHs) inhibited the bacteria transmission.
34974152	10	28	dep	hydrogels	1487:1495	arg1	XGH					1498:1500	XGH	1498:1500	XGH	1498:1500	All of the hydrogels (XGH, KXGH, and VC-KXGHs) inhibited the bacteria transmission.
34974152	10	28	dep	hydrogels	1487:1495	arg1	hydrogels					1487:1495	the hydrogels	1483:1495	the hydrogels (XGH, KXGH, and VC-KXGHs)	1483:1521	All of the hydrogels (XGH, KXGH, and VC-KXGHs) inhibited the bacteria transmission.
34974152	0	29	theme	wound	44:48	arg1	dressings					50:58	Xanthan-gelatin and xanthan-gelatin-keratin wound dressings	0:58	Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of Vitamin C.	0:91	Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of Vitamin C. Recently, functional dressings that can protect the wound area from dehydration and bacterial infection and support healing have gained importance in place of passive dressings.
34974152	7	30	dep	granulating	1217:1227	arg1	moderate					1237:1244	moderate	1237:1244	moderate	1237:1244	Water vapor transmission of XGHs and KXGHs was between 3059.09 ± 126 and 4523 ± 133 g m-2 d-1; therefore, they can be suitable for granulating, low to moderate exudate wounds.
34974152	8	31	theme	exudate	1346:1352	arg1	wounds					1354:1359	exudate wounds	1346:1359	exudate wounds	1346:1359	XGH and KXGHs loaded with VC had higher water uptake, making it more convenient for exudate wounds.
34974152	8	32	theme	water	1302:1306	arg1	uptake					1308:1313	higher water uptake	1295:1313	higher water uptake	1295:1313	XGH and KXGHs loaded with VC had higher water uptake, making it more convenient for exudate wounds.
34974152	9	33	theme	L929	1458:1461	arg1	fibroblasts					1463:1473	L929 fibroblasts	1458:1473	L929 fibroblasts	1458:1473	VC was released for 100 h, and VC containing XGHs and KXGHs increased the collagen synthesis of L929 fibroblasts.
34974152	5	34	theme	mechanical	906:915	arg1	property					917:924	mechanical property	906:924	mechanical property	906:924	X1:GEL1:GLY2 hydrogel was selected for biocompatibility, mechanical property, water vapor transmission rate (WVTR), and porosity.
34974152	0	35	theme	xanthan-gelatin-keratin	20:42	arg1	dressings					50:58	Xanthan-gelatin and xanthan-gelatin-keratin wound dressings	0:58	Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of Vitamin C.	0:91	Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of Vitamin C. Recently, functional dressings that can protect the wound area from dehydration and bacterial infection and support healing have gained importance in place of passive dressings.
34974152	11	36	theme	temporary	1641:1649	arg1	materials					1666:1674	temporary wound dressing materials	1641:1674	temporary wound dressing materials	1641:1674	In conclusion, our results suggest that VC-XGH and VC-KXGH can be candidates for temporary wound dressing materials for skin wounds.
34974152	5	37	dep	X1	849:850	arg1	GEL1					852:855	GEL1	852:855	X1:GEL1:GLY2 hydrogel	849:869	X1:GEL1:GLY2 hydrogel was selected for biocompatibility, mechanical property, water vapor transmission rate (WVTR), and porosity.
34974152	5	37	dep	X1	849:850	arg1	hydrogel					862:869	GLY2 hydrogel	857:869	X1:GEL1:GLY2 hydrogel	849:869	X1:GEL1:GLY2 hydrogel was selected for biocompatibility, mechanical property, water vapor transmission rate (WVTR), and porosity.
34974152	1	38	theme	temporary	299:307	arg1	KXGHs					387:391	KXGHs	387:391	KXGHs	387:391	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	1	38	theme	temporary	299:307	arg1	hydrogels					376:384	temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels	299:384	temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC)	299:515	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	0	39	theme	wound	145:149	arg1	area					151:154	the wound area	141:154	the wound area	141:154	Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of Vitamin C. Recently, functional dressings that can protect the wound area from dehydration and bacterial infection and support healing have gained importance in place of passive dressings.
34974152	8	40	theme	higher	1295:1300	arg1	uptake					1308:1313	higher water uptake	1295:1313	higher water uptake	1295:1313	XGH and KXGHs loaded with VC had higher water uptake, making it more convenient for exudate wounds.
34974152	1	41	theme	high	404:407	arg1	dressing					458:465	a wound dressing	450:465	a wound dressing that can provide local delivery of Vitamin C (VC)	450:515	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	1	41	theme	high	404:407	arg1	applicability					433:445	applicability	433:445	applicability	433:445	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	1	41	theme	high	404:407	arg1	capacity					420:427	high absorption capacity	404:427	high absorption capacity	404:427	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	8	42	contain	had	1291:1293	arg1	XGH					1262:1264	XGH	1262:1264	XGH	1262:1264	XGH and KXGHs loaded with VC had higher water uptake, making it more convenient for exudate wounds.
34974152	8	42	contain	had	1291:1293	arg2	uptake					1308:1313	higher water uptake	1295:1313	higher water uptake	1295:1313	XGH and KXGHs loaded with VC had higher water uptake, making it more convenient for exudate wounds.
34974152	8	42	contain	had	1291:1293	arg1	KXGHs					1270:1274	KXGHs	1270:1274	KXGHs	1270:1274	XGH and KXGHs loaded with VC had higher water uptake, making it more convenient for exudate wounds.
34974152	1	43	theme	regenerative	313:324	arg1	KXGHs					387:391	KXGHs	387:391	KXGHs	387:391	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	1	43	theme	regenerative	313:324	arg1	hydrogels					376:384	temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels	299:384	temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC)	299:515	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	1	44	theme	absorption	409:418	arg1	dressing					458:465	a wound dressing	450:465	a wound dressing that can provide local delivery of Vitamin C (VC)	450:515	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	1	44	theme	absorption	409:418	arg1	applicability					433:445	applicability	433:445	applicability	433:445	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	1	44	theme	absorption	409:418	arg1	capacity					420:427	high absorption capacity	404:427	high absorption capacity	404:427	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	7	45	dep	moderate	1237:1244	arg1	to					1234:1235	to	1234:1235	to	1234:1235	Water vapor transmission of XGHs and KXGHs was between 3059.09 ± 126 and 4523 ± 133 g m-2 d-1; therefore, they can be suitable for granulating, low to moderate exudate wounds.
34974152	1	46	theme	Vitamin	502:508	arg1	C					510:510	Vitamin C	502:510	Vitamin C (VC)	502:515	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	1	46	theme	Vitamin	502:508	arg1	VC					513:514	VC	513:514	VC	513:514	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	0	47	theme	local	64:68	arg1	delivery					70:77	local delivery	64:77	local delivery of Vitamin C.	64:91	Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of Vitamin C. Recently, functional dressings that can protect the wound area from dehydration and bacterial infection and support healing have gained importance in place of passive dressings.
34974152	1	48	theme	xanthan/gelatin	326:340	arg1	KXGHs					387:391	KXGHs	387:391	KXGHs	387:391	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	1	48	theme	xanthan/gelatin	326:340	arg1	hydrogels					376:384	temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels	299:384	temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC)	299:515	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	3	49	theme	weight	698:703	arg1	ratios					705:710	their weight ratios	692:710	their weight ratios	692:710	According to their weight ratios, xanthan, gelatin, and glycerol hydrogels are named.
34974152	4	50	theme	group	809:813	arg1	X1					835:836	X1	835:836	X1:GEL1:GLY2	835:846	If their weight ratio is 1:1:2 (w/w/w), the group name is selected as X1:GEL1:GLY2.
34974152	4	50	theme	group	809:813	arg1	name					815:818	the group name	805:818	the group name	805:818	If their weight ratio is 1:1:2 (w/w/w), the group name is selected as X1:GEL1:GLY2.
34974152	1	51	theme	C	510:510	arg1	delivery					490:497	local delivery	484:497	local delivery of Vitamin C (VC)	484:515	This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC).
34974152	5	52	theme	GLY2	857:860	arg1	hydrogel					862:869	GLY2 hydrogel	857:869	X1:GEL1:GLY2 hydrogel	849:869	X1:GEL1:GLY2 hydrogel was selected for biocompatibility, mechanical property, water vapor transmission rate (WVTR), and porosity.
34974152	11	53	theme	skin	1680:1683	arg1	wounds					1685:1690	skin wounds	1680:1690	skin wounds	1680:1690	In conclusion, our results suggest that VC-XGH and VC-KXGH can be candidates for temporary wound dressing materials for skin wounds.
34974152	9	54	contain	containing	1396:1405	arg2	KXGHs					1416:1420	KXGHs	1416:1420	KXGHs	1416:1420	VC was released for 100 h, and VC containing XGHs and KXGHs increased the collagen synthesis of L929 fibroblasts.
34974152	9	54	contain	containing	1396:1405	arg2	XGHs					1407:1410	XGHs	1407:1410	XGHs	1407:1410	VC was released for 100 h, and VC containing XGHs and KXGHs increased the collagen synthesis of L929 fibroblasts.
34974152	9	54	contain	containing	1396:1405	arg1	VC					1393:1394	VC	1393:1394	VC containing XGHs and KXGHs	1393:1420	VC was released for 100 h, and VC containing XGHs and KXGHs increased the collagen synthesis of L929 fibroblasts.
34974152	5	55	theme	vapor	933:937	arg1	rate					952:955	water vapor transmission rate	927:955	water vapor transmission rate (WVTR)	927:962	X1:GEL1:GLY2 hydrogel was selected for biocompatibility, mechanical property, water vapor transmission rate (WVTR), and porosity.
34974152	5	55	theme	vapor	933:937	arg1	WVTR					958:961	WVTR	958:961	WVTR	958:961	X1:GEL1:GLY2 hydrogel was selected for biocompatibility, mechanical property, water vapor transmission rate (WVTR), and porosity.
34974152	6	56	theme	L929	1028:1031	arg1	fibroblasts					1033:1043	L929 fibroblasts	1028:1043	L929 fibroblasts viability	1028:1053	The addition of keratin to X1:GEL1:GLY2 improved L929 fibroblasts viability and increased protein release.
34974152	9	57	theme	collagen	1436:1443	arg1	synthesis					1445:1453	the collagen synthesis	1432:1453	the collagen synthesis of L929 fibroblasts	1432:1473	VC was released for 100 h, and VC containing XGHs and KXGHs increased the collagen synthesis of L929 fibroblasts.
34974152	5	58	theme	transmission	939:950	arg1	rate					952:955	water vapor transmission rate	927:955	water vapor transmission rate (WVTR)	927:962	X1:GEL1:GLY2 hydrogel was selected for biocompatibility, mechanical property, water vapor transmission rate (WVTR), and porosity.
34974152	5	58	theme	transmission	939:950	arg1	WVTR					958:961	WVTR	958:961	WVTR	958:961	X1:GEL1:GLY2 hydrogel was selected for biocompatibility, mechanical property, water vapor transmission rate (WVTR), and porosity.
34974152	10	59	theme	bacteria	1537:1544	arg1	transmission					1546:1557	the bacteria transmission	1533:1557	the bacteria transmission	1533:1557	All of the hydrogels (XGH, KXGH, and VC-KXGHs) inhibited the bacteria transmission.
34974152	0	60	theme	Vitamin	82:88	arg1	C.					90:91	Vitamin C.	82:91	Vitamin C.	82:91	Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of Vitamin C. Recently, functional dressings that can protect the wound area from dehydration and bacterial infection and support healing have gained importance in place of passive dressings.
34974152	3	61	theme	xanthan	713:719	arg1	hydrogels					744:752	xanthan, gelatin, and glycerol hydrogels	713:752	xanthan, gelatin, and glycerol hydrogels	713:752	According to their weight ratios, xanthan, gelatin, and glycerol hydrogels are named.
34974152	7	62	theme	granulating	1217:1227	arg1	wounds					1254:1259	granulating, low to moderate exudate wounds	1217:1259	granulating, low to moderate exudate wounds	1217:1259	Water vapor transmission of XGHs and KXGHs was between 3059.09 ± 126 and 4523 ± 133 g m-2 d-1; therefore, they can be suitable for granulating, low to moderate exudate wounds.
34974152	7	63	theme	Water	1086:1090	arg1	transmission					1098:1109	Water vapor transmission	1086:1109	Water vapor transmission of XGHs and KXGHs	1086:1127	Water vapor transmission of XGHs and KXGHs was between 3059.09 ± 126 and 4523 ± 133 g m-2 d-1; therefore, they can be suitable for granulating, low to moderate exudate wounds.
34974152	7	64	theme	vapor	1092:1096	arg1	transmission					1098:1109	Water vapor transmission	1086:1109	Water vapor transmission of XGHs and KXGHs	1086:1127	Water vapor transmission of XGHs and KXGHs was between 3059.09 ± 126 and 4523 ± 133 g m-2 d-1; therefore, they can be suitable for granulating, low to moderate exudate wounds.
34974152	2	65	theme	xanthan/gelatin	527:541	arg1	hydrogels					543:551	xanthan/gelatin hydrogels	527:551	xanthan/gelatin hydrogels	527:551	Firstly, xanthan/gelatin hydrogels were produced by crosslinking with different glycerol concentrations and characterized to determine the hydrogel composition.
34400230	2	0	theme	mixed	519:523	arg1	culture					525:531	a mixed culture	517:531	a mixed culture of swine fecal bacteria	517:555	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	1	1	theme	dietary	180:186	arg1	fibers					188:193	soluble dietary fibers	172:193	soluble dietary fibers	172:193	Gut microbial fermentation of soluble dietary fibers promotes general and substrate-specific health benefits.
34400230	7	2	theme	pH	1099:1100	arg1	end					1061:1063	the end	1057:1063	the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation	1057:1158	Further, the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation demonstrated favorable fermentation attributes of agavin compared to RD.
34400230	9	3	theme	fibers	1496:1501	arg1	differences					1469:1479	the structural differences	1454:1479	the structural differences of fermentable fibers	1454:1501	This study revealed a notable significance of the structural differences of fermentable fibers on the subsequent fermentation characteristics.
34400230	6	4	theme	fermentation	973:984	arg1	rates					986:990	the fermentation rates	969:990	the fermentation rates	969:990	Agavin treatment exhibited a clearly segregated two-phased prolonged fermentation trend compared to RD treatment as manifested by the fermentation rates.
34400230	1	5	theme	fibers	188:193	arg1	fermentation					156:167	Gut microbial fermentation	142:167	Gut microbial fermentation of soluble dietary fibers	142:193	Gut microbial fermentation of soluble dietary fibers promotes general and substrate-specific health benefits.
34400230	9	6	from	significance	1438:1449	arg1	characteristics					1534:1548	the subsequent fermentation characteristics	1506:1548	the subsequent fermentation characteristics	1506:1548	This study revealed a notable significance of the structural differences of fermentable fibers on the subsequent fermentation characteristics.
34400230	0	7	theme	batch	115:119	arg1	system					134:139	a simulated batch fermentation system	103:139	a simulated batch fermentation system	103:139	Deciphering the colonic fermentation characteristics of agavin and digestion-resistant maltodextrin in a simulated batch fermentation system.
34400230	7	8	theme	favorable	1173:1181	arg1	attributes					1196:1205	favorable fermentation attributes	1173:1205	favorable fermentation attributes of agavin	1173:1215	Further, the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation demonstrated favorable fermentation attributes of agavin compared to RD.
34400230	3	9	theme	period	594:599	arg1	48 h					564:567	48 h	564:567	48 h of complete fermentation period	564:599	After 48 h of complete fermentation period, the microbial composition was different among all groups, where Bifidobacterium spp.
34400230	2	10	theme	bacteria	548:555	arg1	culture					525:531	a mixed culture	517:531	a mixed culture of swine fecal bacteria	517:555	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	3	11	theme	complete	572:579	arg1	period					594:599	complete fermentation period	572:599	complete fermentation period	572:599	After 48 h of complete fermentation period, the microbial composition was different among all groups, where Bifidobacterium spp.
34400230	7	12	theme	short-chain	1014:1024	arg1	acids					1032:1036	the highest short-chain fatty acids	1002:1036	the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation	1002:1158	Further, the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation demonstrated favorable fermentation attributes of agavin compared to RD.
34400230	2	13	theme	fibers	332:337	arg1	characteristics					284:298	the fermentation characteristics	267:298	the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD)	267:422	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	8	14	theme	microbial	1364:1372	arg1	taxa					1374:1377	the expressed microbial taxa	1350:1377	the expressed microbial taxa	1350:1377	Therefore, agavin might be an effective and desirable substrate for the colonic microbiota than RD with reference to the expressed microbial taxa and fermentation attributes.
34400230	2	15	theme	fecal	542:546	arg1	bacteria					548:555	swine fecal bacteria	536:555	swine fecal bacteria	536:555	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	8	16	theme	expressed	1354:1362	arg1	taxa					1374:1377	the expressed microbial taxa	1350:1377	the expressed microbial taxa	1350:1377	Therefore, agavin might be an effective and desirable substrate for the colonic microbiota than RD with reference to the expressed microbial taxa and fermentation attributes.
34400230	7	17	theme	accumulation	1147:1158	arg1	pH					1099:1100	acidic pH	1092:1100	acidic pH	1092:1100	Further, the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation demonstrated favorable fermentation attributes of agavin compared to RD.
34400230	7	17	theme	accumulation	1147:1158	arg1	concentration					1122:1134	the negligible concentration	1107:1134	the negligible concentration of ammonia accumulation	1107:1158	Further, the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation demonstrated favorable fermentation attributes of agavin compared to RD.
34400230	7	17	theme	accumulation	1147:1158	arg1	cycle					1085:1089	the fermentation cycle	1068:1089	the fermentation cycle	1068:1089	Further, the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation demonstrated favorable fermentation attributes of agavin compared to RD.
34400230	7	18	theme	fatty	1026:1030	arg1	acids					1032:1036	the highest short-chain fatty acids	1002:1036	the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation	1002:1158	Further, the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation demonstrated favorable fermentation attributes of agavin compared to RD.
34400230	2	19	theme	branched-dietary	315:330	arg1	fibers					332:337	two soluble branched-dietary fibers	303:337	two soluble branched-dietary fibers	303:337	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	2	19	theme	branched-dietary	315:330	arg1	maltodextrin					406:417	digestion-resistant maltodextrin	386:417	digestion-resistant maltodextrin (RD)	386:422	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	2	19	theme	branched-dietary	315:330	arg1	agavin					348:353	agavin	348:353	agavin (a type of agave fructans)	348:380	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	8	20	theme	colonic	1305:1311	arg1	microbiota					1313:1322	the colonic microbiota	1301:1322	the colonic microbiota	1301:1322	Therefore, agavin might be an effective and desirable substrate for the colonic microbiota than RD with reference to the expressed microbial taxa and fermentation attributes.
34400230	0	21	theme	fermentation	121:132	arg1	system					134:139	a simulated batch fermentation system	103:139	a simulated batch fermentation system	103:139	Deciphering the colonic fermentation characteristics of agavin and digestion-resistant maltodextrin in a simulated batch fermentation system.
34400230	8	22	theme	desirable	1277:1285	arg1	substrate					1287:1295	an effective and desirable substrate	1260:1295	an effective and desirable substrate for the colonic microbiota than RD with reference to the expressed microbial taxa and fermentation attributes	1260:1405	Therefore, agavin might be an effective and desirable substrate for the colonic microbiota than RD with reference to the expressed microbial taxa and fermentation attributes.
34400230	8	22	theme	desirable	1277:1285	arg1	agavin					1244:1249	agavin	1244:1249	agavin	1244:1249	Therefore, agavin might be an effective and desirable substrate for the colonic microbiota than RD with reference to the expressed microbial taxa and fermentation attributes.
34400230	6	23	theme	Agavin	839:844	arg1	treatment					846:854	Agavin treatment	839:854	Agavin treatment	839:854	Agavin treatment exhibited a clearly segregated two-phased prolonged fermentation trend compared to RD treatment as manifested by the fermentation rates.
34400230	2	24	theme	soluble	307:313	arg1	fibers					332:337	two soluble branched-dietary fibers	303:337	two soluble branched-dietary fibers	303:337	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	2	24	theme	soluble	307:313	arg1	maltodextrin					406:417	digestion-resistant maltodextrin	386:417	digestion-resistant maltodextrin (RD)	386:422	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	2	24	theme	soluble	307:313	arg1	agavin					348:353	agavin	348:353	agavin (a type of agave fructans)	348:380	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	1	25	theme	general	204:210	arg1	benefits					242:249	general and substrate-specific health benefits	204:249	general and substrate-specific health benefits	204:249	Gut microbial fermentation of soluble dietary fibers promotes general and substrate-specific health benefits.
34400230	2	26	theme	simulated	469:477	arg1	apparatus					497:505	a simulated colonic fermenter apparatus	467:505	a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria	467:555	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	8	27	with	RD	1329:1330	arg1	reference					1337:1345	reference	1337:1345	reference to the expressed microbial taxa and fermentation attributes	1337:1405	Therefore, agavin might be an effective and desirable substrate for the colonic microbiota than RD with reference to the expressed microbial taxa and fermentation attributes.
34400230	7	28	theme	highest	1006:1012	arg1	acids					1032:1036	the highest short-chain fatty acids	1002:1036	the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation	1002:1158	Further, the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation demonstrated favorable fermentation attributes of agavin compared to RD.
34400230	7	29	theme	ammonia	1139:1145	arg1	accumulation					1147:1158	ammonia accumulation	1139:1158	ammonia accumulation	1139:1158	Further, the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation demonstrated favorable fermentation attributes of agavin compared to RD.
34400230	6	30	theme	fermentation	908:919	arg1	trend					921:925	a clearly segregated two-phased prolonged fermentation trend	866:925	a clearly segregated two-phased prolonged fermentation trend	866:925	Agavin treatment exhibited a clearly segregated two-phased prolonged fermentation trend compared to RD treatment as manifested by the fermentation rates.
34400230	1	31	theme	substrate-specific	216:233	arg1	benefits					242:249	general and substrate-specific health benefits	204:249	general and substrate-specific health benefits	204:249	Gut microbial fermentation of soluble dietary fibers promotes general and substrate-specific health benefits.
34400230	0	32	theme	fermentation	24:35	arg1	characteristics					37:51	the colonic fermentation characteristics	12:51	the colonic fermentation characteristics of agavin and digestion-resistant maltodextrin	12:98	Deciphering the colonic fermentation characteristics of agavin and digestion-resistant maltodextrin in a simulated batch fermentation system.
34400230	7	33	theme	fermentation	1183:1194	arg1	attributes					1196:1205	favorable fermentation attributes	1173:1205	favorable fermentation attributes of agavin	1173:1215	Further, the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation demonstrated favorable fermentation attributes of agavin compared to RD.
34400230	3	34	theme	fermentation	581:592	arg1	period					594:599	complete fermentation period	572:599	complete fermentation period	572:599	After 48 h of complete fermentation period, the microbial composition was different among all groups, where Bifidobacterium spp.
34400230	1	35	theme	health	235:240	arg1	benefits					242:249	general and substrate-specific health benefits	204:249	general and substrate-specific health benefits	204:249	Gut microbial fermentation of soluble dietary fibers promotes general and substrate-specific health benefits.
34400230	0	36	theme	colonic	16:22	arg1	characteristics					37:51	the colonic fermentation characteristics	12:51	the colonic fermentation characteristics of agavin and digestion-resistant maltodextrin	12:98	Deciphering the colonic fermentation characteristics of agavin and digestion-resistant maltodextrin in a simulated batch fermentation system.
34400230	7	37	theme	concentration	1122:1134	arg1	end					1061:1063	the end	1057:1063	the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation	1057:1158	Further, the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation demonstrated favorable fermentation attributes of agavin compared to RD.
34400230	6	38	theme	RD	939:940	arg1	treatment					942:950	RD treatment	939:950	RD treatment	939:950	Agavin treatment exhibited a clearly segregated two-phased prolonged fermentation trend compared to RD treatment as manifested by the fermentation rates.
34400230	2	39	theme	fructans	372:379	arg1	type					358:361	a type	356:361	a type of agave fructans	356:379	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	9	40	theme	subsequent	1510:1519	arg1	characteristics					1534:1548	the subsequent fermentation characteristics	1506:1548	the subsequent fermentation characteristics	1506:1548	This study revealed a notable significance of the structural differences of fermentable fibers on the subsequent fermentation characteristics.
34400230	8	41	theme	fermentation	1383:1394	arg1	attributes					1396:1405	fermentation attributes	1383:1405	fermentation attributes	1383:1405	Therefore, agavin might be an effective and desirable substrate for the colonic microbiota than RD with reference to the expressed microbial taxa and fermentation attributes.
34400230	5	42	theme	RD	825:826	arg1	treatment					828:836	the RD treatment	821:836	the RD treatment	821:836	dominated the agavin treatment, while the members of the families Lachnospiraceae and Prevotellaceae dominated the RD treatment.
34400230	7	43	from	end	1061:1063	arg1	production					1038:1047	production	1038:1047	production	1038:1047	Further, the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation demonstrated favorable fermentation attributes of agavin compared to RD.
34400230	7	44	theme	agavin	1210:1215	arg1	attributes					1196:1205	favorable fermentation attributes	1173:1205	favorable fermentation attributes of agavin	1173:1215	Further, the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation demonstrated favorable fermentation attributes of agavin compared to RD.
34400230	9	45	theme	fermentation	1521:1532	arg1	characteristics					1534:1548	the subsequent fermentation characteristics	1506:1548	the subsequent fermentation characteristics	1506:1548	This study revealed a notable significance of the structural differences of fermentable fibers on the subsequent fermentation characteristics.
34400230	9	46	theme	notable	1430:1436	arg1	significance					1438:1449	a notable significance	1428:1449	a notable significance of the structural differences of fermentable fibers on the subsequent fermentation characteristics	1428:1548	This study revealed a notable significance of the structural differences of fermentable fibers on the subsequent fermentation characteristics.
34400230	8	47	theme	effective	1263:1271	arg1	substrate					1287:1295	an effective and desirable substrate	1260:1295	an effective and desirable substrate for the colonic microbiota than RD with reference to the expressed microbial taxa and fermentation attributes	1260:1405	Therefore, agavin might be an effective and desirable substrate for the colonic microbiota than RD with reference to the expressed microbial taxa and fermentation attributes.
34400230	8	47	theme	effective	1263:1271	arg1	agavin					1244:1249	agavin	1244:1249	agavin	1244:1249	Therefore, agavin might be an effective and desirable substrate for the colonic microbiota than RD with reference to the expressed microbial taxa and fermentation attributes.
34400230	7	48	theme	fermentation	1072:1083	arg1	cycle					1085:1089	the fermentation cycle	1068:1089	the fermentation cycle	1068:1089	Further, the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation demonstrated favorable fermentation attributes of agavin compared to RD.
34400230	2	49	theme	swine	536:540	arg1	bacteria					548:555	swine fecal bacteria	536:555	swine fecal bacteria	536:555	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	2	50	theme	fermenter	487:495	arg1	apparatus					497:505	a simulated colonic fermenter apparatus	467:505	a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria	467:555	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	5	51	dep	families	767:774	arg1	families					767:774	the families Lachnospiraceae and Prevotellaceae	763:809	the families Lachnospiraceae and Prevotellaceae	763:809	dominated the agavin treatment, while the members of the families Lachnospiraceae and Prevotellaceae dominated the RD treatment.
34400230	5	51	dep	families	767:774	arg1	Prevotellaceae					796:809	Prevotellaceae	796:809	Prevotellaceae	796:809	dominated the agavin treatment, while the members of the families Lachnospiraceae and Prevotellaceae dominated the RD treatment.
34400230	5	51	dep	families	767:774	arg1	Lachnospiraceae					776:790	Lachnospiraceae	776:790	Lachnospiraceae	776:790	dominated the agavin treatment, while the members of the families Lachnospiraceae and Prevotellaceae dominated the RD treatment.
34400230	3	52	theme	microbial	606:614	arg1	different					632:640	different	632:640	different	632:640	After 48 h of complete fermentation period, the microbial composition was different among all groups, where Bifidobacterium spp.
34400230	3	52	theme	microbial	606:614	arg1	composition					616:626	the microbial composition	602:626	the microbial composition	602:626	After 48 h of complete fermentation period, the microbial composition was different among all groups, where Bifidobacterium spp.
34400230	7	53	theme	cycle	1085:1089	arg1	end					1061:1063	the end	1057:1063	the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation	1057:1158	Further, the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation demonstrated favorable fermentation attributes of agavin compared to RD.
34400230	2	54	theme	agave	366:370	arg1	fructans					372:379	agave fructans	366:379	agave fructans	366:379	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	0	55	theme	agavin	56:61	arg1	characteristics					37:51	the colonic fermentation characteristics	12:51	the colonic fermentation characteristics of agavin and digestion-resistant maltodextrin	12:98	Deciphering the colonic fermentation characteristics of agavin and digestion-resistant maltodextrin in a simulated batch fermentation system.
34400230	7	56	theme	negligible	1111:1120	arg1	concentration					1122:1134	the negligible concentration	1107:1134	the negligible concentration of ammonia accumulation	1107:1158	Further, the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation demonstrated favorable fermentation attributes of agavin compared to RD.
34400230	2	57	theme	colonic	479:485	arg1	apparatus					497:505	a simulated colonic fermenter apparatus	467:505	a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria	467:555	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	1	58	theme	Gut	142:144	arg1	fermentation					156:167	Gut microbial fermentation	142:167	Gut microbial fermentation of soluble dietary fibers	142:193	Gut microbial fermentation of soluble dietary fibers promotes general and substrate-specific health benefits.
34400230	5	59	theme	agavin	724:729	arg1	treatment					731:739	the agavin treatment	720:739	the agavin treatment	720:739	dominated the agavin treatment, while the members of the families Lachnospiraceae and Prevotellaceae dominated the RD treatment.
34400230	6	60	theme	prolonged	898:906	arg1	trend					921:925	a clearly segregated two-phased prolonged fermentation trend	866:925	a clearly segregated two-phased prolonged fermentation trend	866:925	Agavin treatment exhibited a clearly segregated two-phased prolonged fermentation trend compared to RD treatment as manifested by the fermentation rates.
34400230	1	61	theme	microbial	146:154	arg1	fermentation					156:167	Gut microbial fermentation	142:167	Gut microbial fermentation of soluble dietary fibers	142:193	Gut microbial fermentation of soluble dietary fibers promotes general and substrate-specific health benefits.
34400230	9	62	theme	structural	1458:1467	arg1	differences					1469:1479	the structural differences	1454:1479	the structural differences of fermentable fibers	1454:1501	This study revealed a notable significance of the structural differences of fermentable fibers on the subsequent fermentation characteristics.
34400230	5	63	theme	families	767:774	arg1	members					752:758	the members	748:758	the members of the families Lachnospiraceae and Prevotellaceae	748:809	dominated the agavin treatment, while the members of the families Lachnospiraceae and Prevotellaceae dominated the RD treatment.
34400230	7	64	theme	acidic	1092:1097	arg1	pH					1099:1100	acidic pH	1092:1100	acidic pH	1092:1100	Further, the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation demonstrated favorable fermentation attributes of agavin compared to RD.
34400230	6	65	theme	two-phased	887:896	arg1	trend					921:925	a clearly segregated two-phased prolonged fermentation trend	866:925	a clearly segregated two-phased prolonged fermentation trend	866:925	Agavin treatment exhibited a clearly segregated two-phased prolonged fermentation trend compared to RD treatment as manifested by the fermentation rates.
34400230	2	66	dep	agavin	348:353	arg1	type					358:361	a type	356:361	a type of agave fructans	356:379	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	7	67	dep	acids	1032:1036	arg1	production					1038:1047	production	1038:1047	production	1038:1047	Further, the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation demonstrated favorable fermentation attributes of agavin compared to RD.
34400230	9	68	theme	differences	1469:1479	arg1	significance					1438:1449	a notable significance	1428:1449	a notable significance of the structural differences of fermentable fibers on the subsequent fermentation characteristics	1428:1548	This study revealed a notable significance of the structural differences of fermentable fibers on the subsequent fermentation characteristics.
34400230	0	69	theme	maltodextrin	87:98	arg1	characteristics					37:51	the colonic fermentation characteristics	12:51	the colonic fermentation characteristics of agavin and digestion-resistant maltodextrin	12:98	Deciphering the colonic fermentation characteristics of agavin and digestion-resistant maltodextrin in a simulated batch fermentation system.
34400230	6	70	theme	segregated	876:885	arg1	trend					921:925	a clearly segregated two-phased prolonged fermentation trend	866:925	a clearly segregated two-phased prolonged fermentation trend	866:925	Agavin treatment exhibited a clearly segregated two-phased prolonged fermentation trend compared to RD treatment as manifested by the fermentation rates.
34400230	2	71	theme	digestion-resistant	386:404	arg1	RD					420:421	RD	420:421	RD	420:421	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	2	71	theme	digestion-resistant	386:404	arg1	fibers					332:337	two soluble branched-dietary fibers	303:337	two soluble branched-dietary fibers	303:337	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	2	71	theme	digestion-resistant	386:404	arg1	maltodextrin					406:417	digestion-resistant maltodextrin	386:417	digestion-resistant maltodextrin (RD)	386:422	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	0	72	theme	digestion-resistant	67:85	arg1	maltodextrin					87:98	digestion-resistant maltodextrin	67:98	digestion-resistant maltodextrin	67:98	Deciphering the colonic fermentation characteristics of agavin and digestion-resistant maltodextrin in a simulated batch fermentation system.
34400230	2	73	theme	fermentation	271:282	arg1	characteristics					284:298	the fermentation characteristics	267:298	the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD)	267:422	In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria.
34400230	1	74	theme	soluble	172:178	arg1	fibers					188:193	soluble dietary fibers	172:193	soluble dietary fibers	172:193	Gut microbial fermentation of soluble dietary fibers promotes general and substrate-specific health benefits.
34400230	9	75	theme	fermentable	1484:1494	arg1	fibers					1496:1501	fermentable fibers	1484:1501	fermentable fibers	1484:1501	This study revealed a notable significance of the structural differences of fermentable fibers on the subsequent fermentation characteristics.
34400230	0	76	theme	simulated	105:113	arg1	system					134:139	a simulated batch fermentation system	103:139	a simulated batch fermentation system	103:139	Deciphering the colonic fermentation characteristics of agavin and digestion-resistant maltodextrin in a simulated batch fermentation system.
34857282	0	0	theme	/α‑calcium	82:91	arg1	sulfate					93:99	carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate	45:99	carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate	45:99	Physicochemical and biological properties of carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate hemihydrate (α-CSH) composites.
34857282	1	1	theme	different	321:329	arg1	ratios					336:341	different mass ratios	321:341	different mass ratios	321:341	To improve the osteoinductivity, antibacterial activity, and clinical application of calcium sulfate hemihydrate (CSH), carboxymethyl chitosan zinc (CMCS-Zn) and α-CSH were prepared using different mass ratios.
34857282	11	2	theme	osteogenesis-promoting	1543:1564	arg1	effects					1566:1572	osteogenesis-promoting effects	1543:1572	osteogenesis-promoting effects	1543:1572	Therefore, CMCS-Zn/α-CSH was demonstrated to be an adjustable, degradable, substitute biomaterial (with osteogenesis-promoting effects) for use in bone defects, which also has antibacterial activity that can suppress bone infection.
34857282	11	3	contain	has	1611:1613	arg1	adjustable					1490:1499	adjustable	1490:1499	adjustable	1490:1499	Therefore, CMCS-Zn/α-CSH was demonstrated to be an adjustable, degradable, substitute biomaterial (with osteogenesis-promoting effects) for use in bone defects, which also has antibacterial activity that can suppress bone infection.
34857282	11	3	contain	has	1611:1613	arg1	CMCS-Zn/α-CSH					1450:1462	CMCS-Zn/α-CSH	1450:1462	CMCS-Zn/α-CSH	1450:1462	Therefore, CMCS-Zn/α-CSH was demonstrated to be an adjustable, degradable, substitute biomaterial (with osteogenesis-promoting effects) for use in bone defects, which also has antibacterial activity that can suppress bone infection.
34857282	11	3	contain	has	1611:1613	arg2	activity					1629:1636	antibacterial activity	1615:1636	antibacterial activity that can suppress bone infection	1615:1669	Therefore, CMCS-Zn/α-CSH was demonstrated to be an adjustable, degradable, substitute biomaterial (with osteogenesis-promoting effects) for use in bone defects, which also has antibacterial activity that can suppress bone infection.
34857282	9	4	theme	CMCS-Zn/α-CSH	1232:1244	arg1	function					1265:1272	a function	1263:1272	a function of CMCS-Zn content	1263:1291	The antibacterial ability of CMCS-Zn/α-CSH was evaluated as a function of CMCS-Zn content.
34857282	9	4	theme	CMCS-Zn/α-CSH	1232:1244	arg1	ability					1221:1227	The antibacterial ability	1203:1227	The antibacterial ability of CMCS-Zn/α-CSH	1203:1244	The antibacterial ability of CMCS-Zn/α-CSH was evaluated as a function of CMCS-Zn content.
34857282	1	5	theme	mass	331:334	arg1	ratios					336:341	different mass ratios	321:341	different mass ratios	321:341	To improve the osteoinductivity, antibacterial activity, and clinical application of calcium sulfate hemihydrate (CSH), carboxymethyl chitosan zinc (CMCS-Zn) and α-CSH were prepared using different mass ratios.
34857282	10	6	theme	%	1325:1325	arg1	group					1341:1345	5% CMCS-Zn/α-CSH group	1324:1345	5% CMCS-Zn/α-CSH group	1324:1345	In the rat bone defect model, 5% CMCS-Zn/α-CSH group revealed a higher volume and density of trabeculae by micro-CT 8 weeks after the operation.
34857282	5	7	theme	FTIR	834:837	arg1	spectroscopy					840:851	Fourier transform (FTIR) spectroscopy	815:851	Fourier transform (FTIR) spectroscopy	815:851	After mixing CMCS-Zn and α-CSH, no new phase was measured by X-ray diffraction (XRD) and Fourier transform (FTIR) spectroscopy.
34857282	10	8	theme	5	1324:1324	arg1	%					1325:1325	%	1325:1325	%	1325:1325	In the rat bone defect model, 5% CMCS-Zn/α-CSH group revealed a higher volume and density of trabeculae by micro-CT 8 weeks after the operation.
34857282	1	9	theme	carboxymethyl	253:265	arg1	CMCS-Zn					282:288	carboxymethyl chitosan zinc (CMCS-Zn)	253:289	carboxymethyl chitosan zinc (CMCS-Zn)	253:289	To improve the osteoinductivity, antibacterial activity, and clinical application of calcium sulfate hemihydrate (CSH), carboxymethyl chitosan zinc (CMCS-Zn) and α-CSH were prepared using different mass ratios.
34857282	0	10	theme	sulfate	93:99	arg1	properties					31:40	Physicochemical and biological properties	0:40	Physicochemical and biological properties of carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate	0:99	Physicochemical and biological properties of carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate hemihydrate (α-CSH) composites.
34857282	9	11	theme	CMCS-Zn	1277:1283	arg1	content					1285:1291	CMCS-Zn content	1277:1291	CMCS-Zn content	1277:1291	The antibacterial ability of CMCS-Zn/α-CSH was evaluated as a function of CMCS-Zn content.
34857282	7	12	theme	Ca2+	1073:1076	arg1	release					1062:1068	the release	1058:1068	the release of Ca2+	1058:1076	The release of Zn2+ increased with increasing CMCS-Zn content, while the release of Ca2+ decreased.
34857282	3	13	theme	CMCS-Zn	541:547	arg1	structure					528:536	the fine lamellar structure	510:536	the fine lamellar structure of CMCS-Zn	510:547	After adding different amounts of CMCS-Zn to α-CSH, the fine lamellar structure of CMCS-Zn was found by scanning electron microscopy (SEM), which is evenly distributed in the matrix of α-CSH.
34857282	1	14	theme	chitosan	267:274	arg1	CMCS-Zn					282:288	carboxymethyl chitosan zinc (CMCS-Zn)	253:289	carboxymethyl chitosan zinc (CMCS-Zn)	253:289	To improve the osteoinductivity, antibacterial activity, and clinical application of calcium sulfate hemihydrate (CSH), carboxymethyl chitosan zinc (CMCS-Zn) and α-CSH were prepared using different mass ratios.
34857282	0	15	theme	α-CSH	114:118	arg1	composites					121:130	(α-CSH) composites	113:130	(α-CSH) composites	113:130	Physicochemical and biological properties of carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate hemihydrate (α-CSH) composites.
34857282	8	16	theme	CMCS-Zn/α-CSH	1101:1113	arg1	composites					1115:1124	CMCS-Zn/α-CSH composites	1101:1124	CMCS-Zn/α-CSH composites	1101:1124	Extracts of CMCS-Zn/α-CSH composites up-regulated the osteoinduction and migration of rat bone marrow stem cells.
34857282	11	17	dep	adjustable	1490:1499	arg1	substitute					1514:1523	substitute	1514:1523	substitute biomaterial (with osteogenesis-promoting effects) for use in bone defects	1514:1597	Therefore, CMCS-Zn/α-CSH was demonstrated to be an adjustable, degradable, substitute biomaterial (with osteogenesis-promoting effects) for use in bone defects, which also has antibacterial activity that can suppress bone infection.
34857282	3	18	theme	lamellar	519:526	arg1	structure					528:536	the fine lamellar structure	510:536	the fine lamellar structure of CMCS-Zn	510:547	After adding different amounts of CMCS-Zn to α-CSH, the fine lamellar structure of CMCS-Zn was found by scanning electron microscopy (SEM), which is evenly distributed in the matrix of α-CSH.
34857282	1	19	theme	antibacterial	166:178	arg1	activity					180:187	antibacterial activity	166:187	antibacterial activity	166:187	To improve the osteoinductivity, antibacterial activity, and clinical application of calcium sulfate hemihydrate (CSH), carboxymethyl chitosan zinc (CMCS-Zn) and α-CSH were prepared using different mass ratios.
34857282	7	20	theme	CMCS-Zn	1035:1041	arg1	content					1043:1049	CMCS-Zn content	1035:1049	CMCS-Zn content	1035:1049	The release of Zn2+ increased with increasing CMCS-Zn content, while the release of Ca2+ decreased.
34857282	0	21	theme	Physicochemical	0:14	arg1	properties					31:40	Physicochemical and biological properties	0:40	Physicochemical and biological properties of carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate	0:99	Physicochemical and biological properties of carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate hemihydrate (α-CSH) composites.
34857282	8	22	theme	bone	1179:1182	arg1	cells					1196:1200	rat bone marrow stem cells	1175:1200	rat bone marrow stem cells	1175:1200	Extracts of CMCS-Zn/α-CSH composites up-regulated the osteoinduction and migration of rat bone marrow stem cells.
34857282	6	23	theme	CMCS-Zn	918:924	arg1	content					926:932	CMCS-Zn content	918:932	CMCS-Zn content	918:932	The degradation rate of CMCS-Zn/α-CSH decreased with increasing CMCS-Zn content, and the pH was stable during the degradation process.
34857282	8	24	theme	cells	1196:1200	arg1	migration					1162:1170	migration	1162:1170	migration	1162:1170	Extracts of CMCS-Zn/α-CSH composites up-regulated the osteoinduction and migration of rat bone marrow stem cells.
34857282	8	24	theme	cells	1196:1200	arg1	osteoinduction					1143:1156	osteoinduction	1143:1156	osteoinduction	1143:1156	Extracts of CMCS-Zn/α-CSH composites up-regulated the osteoinduction and migration of rat bone marrow stem cells.
34857282	2	25	theme	CMCS-Zn	441:447	arg1	content					449:455	CMCS-Zn content	441:455	CMCS-Zn content	441:455	The setting time and injectability of the CMCS-Zn/α-CSH composite were increased with increasing CMCS-Zn content.
34857282	1	26	theme	zinc	276:279	arg1	CMCS-Zn					282:288	carboxymethyl chitosan zinc (CMCS-Zn)	253:289	carboxymethyl chitosan zinc (CMCS-Zn)	253:289	To improve the osteoinductivity, antibacterial activity, and clinical application of calcium sulfate hemihydrate (CSH), carboxymethyl chitosan zinc (CMCS-Zn) and α-CSH were prepared using different mass ratios.
34857282	3	27	theme	scanning	562:569	arg1	SEM					592:594	SEM	592:594	SEM	592:594	After adding different amounts of CMCS-Zn to α-CSH, the fine lamellar structure of CMCS-Zn was found by scanning electron microscopy (SEM), which is evenly distributed in the matrix of α-CSH.
34857282	3	27	theme	scanning	562:569	arg1	microscopy					580:589	scanning electron microscopy	562:589	scanning electron microscopy (SEM)	562:595	After adding different amounts of CMCS-Zn to α-CSH, the fine lamellar structure of CMCS-Zn was found by scanning electron microscopy (SEM), which is evenly distributed in the matrix of α-CSH.
34857282	8	28	theme	stem	1191:1194	arg1	cells					1196:1200	rat bone marrow stem cells	1175:1200	rat bone marrow stem cells	1175:1200	Extracts of CMCS-Zn/α-CSH composites up-regulated the osteoinduction and migration of rat bone marrow stem cells.
34857282	5	29	theme	new	761:763	arg1	phase					765:769	no new phase	758:769	no new phase	758:769	After mixing CMCS-Zn and α-CSH, no new phase was measured by X-ray diffraction (XRD) and Fourier transform (FTIR) spectroscopy.
34857282	10	30	theme	CMCS-Zn/α-CSH	1327:1339	arg1	group					1341:1345	5% CMCS-Zn/α-CSH group	1324:1345	5% CMCS-Zn/α-CSH group	1324:1345	In the rat bone defect model, 5% CMCS-Zn/α-CSH group revealed a higher volume and density of trabeculae by micro-CT 8 weeks after the operation.
34857282	6	31	theme	degradation	968:978	arg1	process					980:986	the degradation process	964:986	the degradation process	964:986	The degradation rate of CMCS-Zn/α-CSH decreased with increasing CMCS-Zn content, and the pH was stable during the degradation process.
34857282	1	32	theme	clinical	194:201	arg1	application					203:213	clinical application	194:213	clinical application of calcium sulfate hemihydrate (CSH)	194:250	To improve the osteoinductivity, antibacterial activity, and clinical application of calcium sulfate hemihydrate (CSH), carboxymethyl chitosan zinc (CMCS-Zn) and α-CSH were prepared using different mass ratios.
34857282	0	33	theme	biological	20:29	arg1	properties					31:40	Physicochemical and biological properties	0:40	Physicochemical and biological properties of carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate	0:99	Physicochemical and biological properties of carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate hemihydrate (α-CSH) composites.
34857282	7	34	theme	Zn2+	1004:1007	arg1	release					993:999	The release	989:999	The release of Zn2+	989:1007	The release of Zn2+ increased with increasing CMCS-Zn content, while the release of Ca2+ decreased.
34857282	3	35	theme	different	471:479	arg1	CMCS-Zn					492:498	CMCS-Zn	492:498	CMCS-Zn	492:498	After adding different amounts of CMCS-Zn to α-CSH, the fine lamellar structure of CMCS-Zn was found by scanning electron microscopy (SEM), which is evenly distributed in the matrix of α-CSH.
34857282	3	35	theme	different	471:479	arg1	amounts					481:487	different amounts	471:487	different amounts of CMCS-Zn	471:498	After adding different amounts of CMCS-Zn to α-CSH, the fine lamellar structure of CMCS-Zn was found by scanning electron microscopy (SEM), which is evenly distributed in the matrix of α-CSH.
34857282	0	36	theme	carboxymethyl	45:57	arg1	sulfate					93:99	carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate	45:99	carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate	45:99	Physicochemical and biological properties of carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate hemihydrate (α-CSH) composites.
34857282	5	37	theme	X-ray	787:791	arg1	XRD					806:808	XRD	806:808	XRD	806:808	After mixing CMCS-Zn and α-CSH, no new phase was measured by X-ray diffraction (XRD) and Fourier transform (FTIR) spectroscopy.
34857282	5	37	theme	X-ray	787:791	arg1	diffraction					793:803	X-ray diffraction	787:803	X-ray diffraction (XRD)	787:809	After mixing CMCS-Zn and α-CSH, no new phase was measured by X-ray diffraction (XRD) and Fourier transform (FTIR) spectroscopy.
34857282	11	38	from	use	1579:1581	arg1	defects					1591:1597	bone defects	1586:1597	bone defects	1586:1597	Therefore, CMCS-Zn/α-CSH was demonstrated to be an adjustable, degradable, substitute biomaterial (with osteogenesis-promoting effects) for use in bone defects, which also has antibacterial activity that can suppress bone infection.
34857282	2	39	theme	composite	400:408	arg1	injectability					365:377	injectability	365:377	injectability	365:377	The setting time and injectability of the CMCS-Zn/α-CSH composite were increased with increasing CMCS-Zn content.
34857282	2	39	theme	composite	400:408	arg1	time					356:359	setting time	348:359	setting time	348:359	The setting time and injectability of the CMCS-Zn/α-CSH composite were increased with increasing CMCS-Zn content.
34857282	8	40	theme	marrow	1184:1189	arg1	cells					1196:1200	rat bone marrow stem cells	1175:1200	rat bone marrow stem cells	1175:1200	Extracts of CMCS-Zn/α-CSH composites up-regulated the osteoinduction and migration of rat bone marrow stem cells.
34857282	6	41	theme	CMCS-Zn/α-CSH	878:890	arg1	rate					870:873	The degradation rate	854:873	The degradation rate of CMCS-Zn/α-CSH	854:890	The degradation rate of CMCS-Zn/α-CSH decreased with increasing CMCS-Zn content, and the pH was stable during the degradation process.
34857282	10	42	dep	higher	1358:1363	arg1	density					1376:1382	density	1376:1382	density	1376:1382	In the rat bone defect model, 5% CMCS-Zn/α-CSH group revealed a higher volume and density of trabeculae by micro-CT 8 weeks after the operation.
34857282	10	42	dep	higher	1358:1363	arg1	volume					1365:1370	volume	1365:1370	volume	1365:1370	In the rat bone defect model, 5% CMCS-Zn/α-CSH group revealed a higher volume and density of trabeculae by micro-CT 8 weeks after the operation.
34857282	11	43	theme	bone	1656:1659	arg1	infection					1661:1669	bone infection	1656:1669	bone infection	1656:1669	Therefore, CMCS-Zn/α-CSH was demonstrated to be an adjustable, degradable, substitute biomaterial (with osteogenesis-promoting effects) for use in bone defects, which also has antibacterial activity that can suppress bone infection.
34857282	1	44	theme	calcium	218:224	arg1	CSH					247:249	CSH	247:249	CSH	247:249	To improve the osteoinductivity, antibacterial activity, and clinical application of calcium sulfate hemihydrate (CSH), carboxymethyl chitosan zinc (CMCS-Zn) and α-CSH were prepared using different mass ratios.
34857282	1	44	theme	calcium	218:224	arg1	hemihydrate					234:244	calcium sulfate hemihydrate	218:244	calcium sulfate hemihydrate (CSH)	218:250	To improve the osteoinductivity, antibacterial activity, and clinical application of calcium sulfate hemihydrate (CSH), carboxymethyl chitosan zinc (CMCS-Zn) and α-CSH were prepared using different mass ratios.
34857282	0	45	theme	zinc	68:71	arg1	sulfate					93:99	carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate	45:99	carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate	45:99	Physicochemical and biological properties of carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate hemihydrate (α-CSH) composites.
34857282	8	46	dep	osteoinduction	1143:1156	arg1	the					1139:1141	the	1139:1141	the	1139:1141	Extracts of CMCS-Zn/α-CSH composites up-regulated the osteoinduction and migration of rat bone marrow stem cells.
34857282	9	47	theme	content	1285:1291	arg1	function					1265:1272	a function	1263:1272	a function of CMCS-Zn content	1263:1291	The antibacterial ability of CMCS-Zn/α-CSH was evaluated as a function of CMCS-Zn content.
34857282	9	47	theme	content	1285:1291	arg1	ability					1221:1227	The antibacterial ability	1203:1227	The antibacterial ability of CMCS-Zn/α-CSH	1203:1244	The antibacterial ability of CMCS-Zn/α-CSH was evaluated as a function of CMCS-Zn content.
34857282	3	48	theme	electron	571:578	arg1	SEM					592:594	SEM	592:594	SEM	592:594	After adding different amounts of CMCS-Zn to α-CSH, the fine lamellar structure of CMCS-Zn was found by scanning electron microscopy (SEM), which is evenly distributed in the matrix of α-CSH.
34857282	3	48	theme	electron	571:578	arg1	microscopy					580:589	scanning electron microscopy	562:589	scanning electron microscopy (SEM)	562:595	After adding different amounts of CMCS-Zn to α-CSH, the fine lamellar structure of CMCS-Zn was found by scanning electron microscopy (SEM), which is evenly distributed in the matrix of α-CSH.
34857282	10	49	theme	defect	1310:1315	arg1	model					1317:1321	the rat bone defect model	1297:1321	the rat bone defect model	1297:1321	In the rat bone defect model, 5% CMCS-Zn/α-CSH group revealed a higher volume and density of trabeculae by micro-CT 8 weeks after the operation.
34857282	3	50	theme	CMCS-Zn	492:498	arg1	CMCS-Zn					492:498	CMCS-Zn	492:498	CMCS-Zn	492:498	After adding different amounts of CMCS-Zn to α-CSH, the fine lamellar structure of CMCS-Zn was found by scanning electron microscopy (SEM), which is evenly distributed in the matrix of α-CSH.
34857282	3	50	theme	CMCS-Zn	492:498	arg1	amounts					481:487	different amounts	471:487	different amounts of CMCS-Zn	471:498	After adding different amounts of CMCS-Zn to α-CSH, the fine lamellar structure of CMCS-Zn was found by scanning electron microscopy (SEM), which is evenly distributed in the matrix of α-CSH.
34857282	0	51	theme	chitosan	59:66	arg1	sulfate					93:99	carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate	45:99	carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate	45:99	Physicochemical and biological properties of carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate hemihydrate (α-CSH) composites.
34857282	3	52	theme	α-CSH	643:647	arg1	matrix					633:638	the matrix	629:638	the matrix of α-CSH	629:647	After adding different amounts of CMCS-Zn to α-CSH, the fine lamellar structure of CMCS-Zn was found by scanning electron microscopy (SEM), which is evenly distributed in the matrix of α-CSH.
34857282	4	53	theme	CMCS-Zn	671:677	arg1	increase					659:666	the increase	655:666	the increase of CMCS-Zn	655:677	With the increase of CMCS-Zn, the pores on the surface gradually increased.
34857282	4	54	from	pores	684:688	arg1	surface					697:703	the surface	693:703	the surface	693:703	With the increase of CMCS-Zn, the pores on the surface gradually increased.
34857282	6	55	theme	degradation	858:868	arg1	rate					870:873	The degradation rate	854:873	The degradation rate of CMCS-Zn/α-CSH	854:890	The degradation rate of CMCS-Zn/α-CSH decreased with increasing CMCS-Zn content, and the pH was stable during the degradation process.
34857282	1	56	theme	sulfate	226:232	arg1	CSH					247:249	CSH	247:249	CSH	247:249	To improve the osteoinductivity, antibacterial activity, and clinical application of calcium sulfate hemihydrate (CSH), carboxymethyl chitosan zinc (CMCS-Zn) and α-CSH were prepared using different mass ratios.
34857282	1	56	theme	sulfate	226:232	arg1	hemihydrate					234:244	calcium sulfate hemihydrate	218:244	calcium sulfate hemihydrate (CSH)	218:250	To improve the osteoinductivity, antibacterial activity, and clinical application of calcium sulfate hemihydrate (CSH), carboxymethyl chitosan zinc (CMCS-Zn) and α-CSH were prepared using different mass ratios.
34857282	5	57	dep	spectroscopy	840:851	arg1	transform					823:831	transform	823:831	transform	823:831	After mixing CMCS-Zn and α-CSH, no new phase was measured by X-ray diffraction (XRD) and Fourier transform (FTIR) spectroscopy.
34857282	9	58	theme	antibacterial	1207:1219	arg1	function					1265:1272	a function	1263:1272	a function of CMCS-Zn content	1263:1291	The antibacterial ability of CMCS-Zn/α-CSH was evaluated as a function of CMCS-Zn content.
34857282	9	58	theme	antibacterial	1207:1219	arg1	ability					1221:1227	The antibacterial ability	1203:1227	The antibacterial ability of CMCS-Zn/α-CSH	1203:1244	The antibacterial ability of CMCS-Zn/α-CSH was evaluated as a function of CMCS-Zn content.
34857282	8	59	theme	composites	1115:1124	arg1	Extracts					1089:1096	Extracts	1089:1096	Extracts of CMCS-Zn/α-CSH composites	1089:1124	Extracts of CMCS-Zn/α-CSH composites up-regulated the osteoinduction and migration of rat bone marrow stem cells.
34857282	10	60	theme	micro-CT	1401:1408	arg1	8 weeks					1410:1416	micro-CT 8 weeks	1401:1416	micro-CT 8 weeks after the operation	1401:1436	In the rat bone defect model, 5% CMCS-Zn/α-CSH group revealed a higher volume and density of trabeculae by micro-CT 8 weeks after the operation.
34857282	3	61	theme	fine	514:517	arg1	structure					528:536	the fine lamellar structure	510:536	the fine lamellar structure of CMCS-Zn	510:547	After adding different amounts of CMCS-Zn to α-CSH, the fine lamellar structure of CMCS-Zn was found by scanning electron microscopy (SEM), which is evenly distributed in the matrix of α-CSH.
34857282	11	62	theme	bone	1586:1589	arg1	defects					1591:1597	bone defects	1586:1597	bone defects	1586:1597	Therefore, CMCS-Zn/α-CSH was demonstrated to be an adjustable, degradable, substitute biomaterial (with osteogenesis-promoting effects) for use in bone defects, which also has antibacterial activity that can suppress bone infection.
34857282	1	63	theme	hemihydrate	234:244	arg1	application					203:213	clinical application	194:213	clinical application of calcium sulfate hemihydrate (CSH)	194:250	To improve the osteoinductivity, antibacterial activity, and clinical application of calcium sulfate hemihydrate (CSH), carboxymethyl chitosan zinc (CMCS-Zn) and α-CSH were prepared using different mass ratios.
34857282	1	63	theme	hemihydrate	234:244	arg1	osteoinductivity					148:163	the osteoinductivity	144:163	the osteoinductivity	144:163	To improve the osteoinductivity, antibacterial activity, and clinical application of calcium sulfate hemihydrate (CSH), carboxymethyl chitosan zinc (CMCS-Zn) and α-CSH were prepared using different mass ratios.
34857282	1	63	theme	hemihydrate	234:244	arg1	activity					180:187	antibacterial activity	166:187	antibacterial activity	166:187	To improve the osteoinductivity, antibacterial activity, and clinical application of calcium sulfate hemihydrate (CSH), carboxymethyl chitosan zinc (CMCS-Zn) and α-CSH were prepared using different mass ratios.
34857282	0	64	theme	CMCS-Zn	74:80	arg1	sulfate					93:99	carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate	45:99	carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate	45:99	Physicochemical and biological properties of carboxymethyl chitosan zinc (CMCS-Zn)/α‑calcium sulfate hemihydrate (α-CSH) composites.
34857282	2	65	theme	CMCS-Zn/α-CSH	386:398	arg1	composite					400:408	the CMCS-Zn/α-CSH composite	382:408	the CMCS-Zn/α-CSH composite	382:408	The setting time and injectability of the CMCS-Zn/α-CSH composite were increased with increasing CMCS-Zn content.
34857282	10	66	theme	bone	1305:1308	arg1	model					1317:1321	the rat bone defect model	1297:1321	the rat bone defect model	1297:1321	In the rat bone defect model, 5% CMCS-Zn/α-CSH group revealed a higher volume and density of trabeculae by micro-CT 8 weeks after the operation.
34857282	5	67	theme	Fourier	815:821	arg1	spectroscopy					840:851	Fourier transform (FTIR) spectroscopy	815:851	Fourier transform (FTIR) spectroscopy	815:851	After mixing CMCS-Zn and α-CSH, no new phase was measured by X-ray diffraction (XRD) and Fourier transform (FTIR) spectroscopy.
34857282	8	68	theme	rat	1175:1177	arg1	cells					1196:1200	rat bone marrow stem cells	1175:1200	rat bone marrow stem cells	1175:1200	Extracts of CMCS-Zn/α-CSH composites up-regulated the osteoinduction and migration of rat bone marrow stem cells.
34857282	11	69	with	biomaterial	1525:1535	arg1	effects					1566:1572	osteogenesis-promoting effects	1543:1572	osteogenesis-promoting effects	1543:1572	Therefore, CMCS-Zn/α-CSH was demonstrated to be an adjustable, degradable, substitute biomaterial (with osteogenesis-promoting effects) for use in bone defects, which also has antibacterial activity that can suppress bone infection.
34857282	10	70	theme	rat	1301:1303	arg1	model					1317:1321	the rat bone defect model	1297:1321	the rat bone defect model	1297:1321	In the rat bone defect model, 5% CMCS-Zn/α-CSH group revealed a higher volume and density of trabeculae by micro-CT 8 weeks after the operation.
34857282	2	71	theme	setting	348:354	arg1	time					356:359	setting time	348:359	setting time	348:359	The setting time and injectability of the CMCS-Zn/α-CSH composite were increased with increasing CMCS-Zn content.
34857282	11	72	theme	antibacterial	1615:1627	arg1	activity					1629:1636	antibacterial activity	1615:1636	antibacterial activity that can suppress bone infection	1615:1669	Therefore, CMCS-Zn/α-CSH was demonstrated to be an adjustable, degradable, substitute biomaterial (with osteogenesis-promoting effects) for use in bone defects, which also has antibacterial activity that can suppress bone infection.
32522544	9	0	theme	AD	1423:1424	arg1	pathology					1426:1434	AD pathology	1423:1434	AD pathology	1423:1434	Moreover, M-β-Glu and S-β-Glu ameliorated neuroinflammation and brain insulin resistance (IR), which played a central role in the process of AD pathology.
32522544	10	1	theme	gut	1608:1610	arg1	microbiota					1612:1621	gut microbiota	1608:1621	gut microbiota	1608:1621	This study broadened the underlying applications of yeast β-glucans as a novel dietary supplementation to prevent early-stage pathologies associated with AD by regulating gut microbiota and the potential mechanism might be ameliorating brain IR.
32522544	10	2	theme	potential	1631:1639	arg1	mechanism					1641:1649	the potential mechanism	1627:1649	the potential mechanism	1627:1649	This study broadened the underlying applications of yeast β-glucans as a novel dietary supplementation to prevent early-stage pathologies associated with AD by regulating gut microbiota and the potential mechanism might be ameliorating brain IR.
32522544	3	3	from	effects	411:417	arg1	diseases					459:466	neurodegenerative diseases	441:466	neurodegenerative diseases	441:466	However, few studies had investigated the effects of yeast β-glucans on neurodegenerative diseases.
32522544	10	4	theme	early-stage	1551:1561	arg1	pathologies					1563:1573	early-stage pathologies	1551:1573	early-stage pathologies associated with AD	1551:1592	This study broadened the underlying applications of yeast β-glucans as a novel dietary supplementation to prevent early-stage pathologies associated with AD by regulating gut microbiota and the potential mechanism might be ameliorating brain IR.
32522544	2	5	theme	biological	349:358	arg1	effects					360:366	their biological effects	343:366	their biological effects	343:366	Yeast β-glucans have been incorporated in functional foods and used in prophylactic applications owing to their biological effects.
32522544	0	6	theme	AD-like	107:113	arg1	mice					115:118	Aβ1-42-induced AD-like mice	92:118	Aβ1-42-induced AD-like mice	92:118	Yeast β-glucan alleviates cognitive deficit by regulating gut microbiota and metabolites in Aβ1-42-induced AD-like mice.
32522544	6	7	theme	inflammatory-related	939:958	arg1	bacteria					960:967	inflammatory-related bacteria	939:967	inflammatory-related bacteria	939:967	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	6	7	theme	inflammatory-related	939:958	arg1	Desulfovibrio					1009:1021	Desulfovibrio	1009:1021	Desulfovibrio	1009:1021	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	6	7	theme	inflammatory-related	939:958	arg1	Anaerotruncus					1065:1077	Anaerotruncus	1065:1077	Anaerotruncus	1065:1077	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	6	7	theme	inflammatory-related	939:958	arg1	Lactobacillus					977:989	Lactobacillus	977:989	Lactobacillus	977:989	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	6	7	theme	inflammatory-related	939:958	arg1	Rikenella					1084:1092	Rikenella	1084:1092	Rikenella	1084:1092	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	6	7	theme	inflammatory-related	939:958	arg1	Bifidobacterium					992:1006	Bifidobacterium	992:1006	Bifidobacterium	992:1006	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	6	7	theme	inflammatory-related	939:958	arg1	Mucispirillum					1039:1051	Mucispirillum	1039:1051	Mucispirillum	1039:1051	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	10	8	theme	dietary	1516:1522	arg1	supplementation					1524:1538	a novel dietary supplementation	1508:1538	a novel dietary supplementation	1508:1538	This study broadened the underlying applications of yeast β-glucans as a novel dietary supplementation to prevent early-stage pathologies associated with AD by regulating gut microbiota and the potential mechanism might be ameliorating brain IR.
32522544	5	9	theme	yeast	623:627	arg1	β-glucans					629:637	yeast β-glucans	623:637	yeast β-glucans	623:637	Results indicated that yeast β-glucans could prominently shape the intestinal flora and produce SCFAs.
32522544	8	10	theme	AD-related	1244:1253	arg1	pathologies					1255:1265	AD-related pathologies	1244:1265	AD-related pathologies	1244:1265	Correlation analysis demonstrated the key microbiota closely associated with AD-related pathologies and cognition.
32522544	0	11	theme	Aβ1-42-induced	92:105	arg1	mice					115:118	Aβ1-42-induced AD-like mice	92:118	Aβ1-42-induced AD-like mice	92:118	Yeast β-glucan alleviates cognitive deficit by regulating gut microbiota and metabolites in Aβ1-42-induced AD-like mice.
32522544	9	12	theme	central	1392:1398	arg1	role					1400:1403	a central role	1390:1403	a central role	1390:1403	Moreover, M-β-Glu and S-β-Glu ameliorated neuroinflammation and brain insulin resistance (IR), which played a central role in the process of AD pathology.
32522544	10	13	theme	novel	1510:1514	arg1	supplementation					1524:1538	a novel dietary supplementation	1508:1538	a novel dietary supplementation	1508:1538	This study broadened the underlying applications of yeast β-glucans as a novel dietary supplementation to prevent early-stage pathologies associated with AD by regulating gut microbiota and the potential mechanism might be ameliorating brain IR.
32522544	10	14	theme	underlying	1462:1471	arg1	applications					1473:1484	the underlying applications	1458:1484	the underlying applications of yeast β-glucans as a novel dietary supplementation	1458:1538	This study broadened the underlying applications of yeast β-glucans as a novel dietary supplementation to prevent early-stage pathologies associated with AD by regulating gut microbiota and the potential mechanism might be ameliorating brain IR.
32522544	2	15	theme	functional	279:288	arg1	foods					290:294	functional foods	279:294	functional foods	279:294	Yeast β-glucans have been incorporated in functional foods and used in prophylactic applications owing to their biological effects.
32522544	6	16	theme	yeast	755:759	arg1	S-β-Glu					771:777	S-β-Glu	771:777	S-β-Glu	771:777	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	6	16	theme	yeast	755:759	arg1	β-glucan					761:768	small-molecular yeast β-glucan	739:768	small-molecular yeast β-glucan (S-β-Glu)	739:778	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	10	17	theme	brain	1673:1677	arg1	IR					1679:1680	brain IR	1673:1680	brain IR	1673:1680	This study broadened the underlying applications of yeast β-glucans as a novel dietary supplementation to prevent early-stage pathologies associated with AD by regulating gut microbiota and the potential mechanism might be ameliorating brain IR.
32522544	4	18	theme	gut	475:477	arg1	microbiota					479:488	gut microbiota	475:488	gut microbiota	475:488	Here, gut microbiota and metabolites SCFAs were analyzed through high-throughput 16S rRNA gene sequencing and GC-MS, respectively.
32522544	6	19	theme	evident	834:840	arg1	alterations					842:852	evident alterations	834:852	evident alterations of the composition of the gut microbiota	834:893	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	6	20	theme	small-molecular	739:753	arg1	S-β-Glu					771:777	S-β-Glu	771:777	S-β-Glu	771:777	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	6	20	theme	small-molecular	739:753	arg1	β-glucan					761:768	small-molecular yeast β-glucan	739:768	small-molecular yeast β-glucan (S-β-Glu)	739:778	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	1	21	theme	neurodegenerative	151:167	arg1	disease					133:139	Alzheimer's disease	121:139	Alzheimer's disease (AD)	121:144	Alzheimer's disease (AD) is a neurodegenerative disease that remarkably imposes a huge global public health burden.
32522544	1	21	theme	neurodegenerative	151:167	arg1	disease					169:175	a neurodegenerative disease	149:175	a neurodegenerative disease that remarkably imposes a huge global public health burden	149:234	Alzheimer's disease (AD) is a neurodegenerative disease that remarkably imposes a huge global public health burden.
32522544	6	22	theme	beneficial	915:924	arg1	Desulfovibrio					1009:1021	Desulfovibrio	1009:1021	Desulfovibrio	1009:1021	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	6	22	theme	beneficial	915:924	arg1	Anaerotruncus					1065:1077	Anaerotruncus	1065:1077	Anaerotruncus	1065:1077	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	6	22	theme	beneficial	915:924	arg1	bacteria					926:933	some beneficial bacteria	910:933	some beneficial bacteria	910:933	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	6	22	theme	beneficial	915:924	arg1	Lactobacillus					977:989	Lactobacillus	977:989	Lactobacillus	977:989	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	6	22	theme	beneficial	915:924	arg1	Rikenella					1084:1092	Rikenella	1084:1092	Rikenella	1084:1092	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	6	22	theme	beneficial	915:924	arg1	Bifidobacterium					992:1006	Bifidobacterium	992:1006	Bifidobacterium	992:1006	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	6	22	theme	beneficial	915:924	arg1	Mucispirillum					1039:1051	Mucispirillum	1039:1051	Mucispirillum	1039:1051	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	6	23	theme	microbiota	884:893	arg1	composition					861:871	the composition	857:871	the composition of the gut microbiota	857:893	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	3	24	theme	neurodegenerative	441:457	arg1	diseases					459:466	neurodegenerative diseases	441:466	neurodegenerative diseases	441:466	However, few studies had investigated the effects of yeast β-glucans on neurodegenerative diseases.
32522544	7	25	theme	better	1146:1151	arg1	microbiota					1117:1126	M-β-Glu regulated gut microbiota	1095:1126	M-β-Glu regulated gut microbiota	1095:1126	M-β-Glu regulated gut microbiota act as prebiotics better than S-β-Glu.
32522544	7	25	theme	better	1146:1151	arg1	prebiotics					1135:1144	prebiotics	1135:1144	prebiotics better than S-β-Glu	1135:1164	M-β-Glu regulated gut microbiota act as prebiotics better than S-β-Glu.
32522544	2	26	theme	prophylactic	308:319	arg1	applications					321:332	prophylactic applications	308:332	prophylactic applications owing to their biological effects	308:366	Yeast β-glucans have been incorporated in functional foods and used in prophylactic applications owing to their biological effects.
32522544	8	27	theme	Correlation	1167:1177	arg1	analysis					1179:1186	Correlation analysis	1167:1186	Correlation analysis	1167:1186	Correlation analysis demonstrated the key microbiota closely associated with AD-related pathologies and cognition.
32522544	3	28	theme	yeast	422:426	arg1	β-glucans					428:436	yeast β-glucans	422:436	yeast β-glucans	422:436	However, few studies had investigated the effects of yeast β-glucans on neurodegenerative diseases.
32522544	0	29	theme	Yeast	0:4	arg1	β-glucan					6:13	Yeast β-glucan	0:13	Yeast β-glucan	0:13	Yeast β-glucan alleviates cognitive deficit by regulating gut microbiota and metabolites in Aβ1-42-induced AD-like mice.
32522544	5	30	theme	intestinal	667:676	arg1	flora					678:682	the intestinal flora	663:682	the intestinal flora	663:682	Results indicated that yeast β-glucans could prominently shape the intestinal flora and produce SCFAs.
32522544	10	31	theme	β-glucans	1495:1503	arg1	applications					1473:1484	the underlying applications	1458:1484	the underlying applications of yeast β-glucans as a novel dietary supplementation	1458:1538	This study broadened the underlying applications of yeast β-glucans as a novel dietary supplementation to prevent early-stage pathologies associated with AD by regulating gut microbiota and the potential mechanism might be ameliorating brain IR.
32522544	3	32	theme	β-glucans	428:436	arg1	effects					411:417	the effects	407:417	the effects of yeast β-glucans on neurodegenerative diseases	407:466	However, few studies had investigated the effects of yeast β-glucans on neurodegenerative diseases.
32522544	0	33	theme	cognitive	26:34	arg1	deficit					36:42	cognitive deficit	26:42	cognitive deficit	26:42	Yeast β-glucan alleviates cognitive deficit by regulating gut microbiota and metabolites in Aβ1-42-induced AD-like mice.
32522544	10	34	theme	yeast	1489:1493	arg1	β-glucans					1495:1503	yeast β-glucans	1489:1503	yeast β-glucans	1489:1503	This study broadened the underlying applications of yeast β-glucans as a novel dietary supplementation to prevent early-stage pathologies associated with AD by regulating gut microbiota and the potential mechanism might be ameliorating brain IR.
32522544	6	35	theme	AD	718:719	arg1	mice					721:724	Aβ1-42-induced AD mice	703:724	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu)	703:822	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	3	36	theme	few	378:380	arg1	studies					382:388	few studies	378:388	few studies	378:388	However, few studies had investigated the effects of yeast β-glucans on neurodegenerative diseases.
32522544	4	37	dep	microbiota	479:488	arg1	SCFAs					506:510	SCFAs	506:510	SCFAs	506:510	Here, gut microbiota and metabolites SCFAs were analyzed through high-throughput 16S rRNA gene sequencing and GC-MS, respectively.
32522544	9	38	theme	insulin	1352:1358	arg1	IR					1372:1373	IR	1372:1373	IR	1372:1373	Moreover, M-β-Glu and S-β-Glu ameliorated neuroinflammation and brain insulin resistance (IR), which played a central role in the process of AD pathology.
32522544	9	38	theme	insulin	1352:1358	arg1	resistance					1360:1369	brain insulin resistance	1346:1369	brain insulin resistance (IR)	1346:1374	Moreover, M-β-Glu and S-β-Glu ameliorated neuroinflammation and brain insulin resistance (IR), which played a central role in the process of AD pathology.
32522544	6	39	theme	Aβ1-42-induced	703:716	arg1	mice					721:724	Aβ1-42-induced AD mice	703:724	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu)	703:822	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	9	40	theme	pathology	1426:1434	arg1	process					1412:1418	the process	1408:1418	the process of AD pathology	1408:1434	Moreover, M-β-Glu and S-β-Glu ameliorated neuroinflammation and brain insulin resistance (IR), which played a central role in the process of AD pathology.
32522544	0	41	from	metabolites	77:87	arg1	mice					115:118	Aβ1-42-induced AD-like mice	92:118	Aβ1-42-induced AD-like mice	92:118	Yeast β-glucan alleviates cognitive deficit by regulating gut microbiota and metabolites in Aβ1-42-induced AD-like mice.
32522544	1	42	theme	huge	203:206	arg1	burden					229:234	a huge global public health burden	201:234	a huge global public health burden	201:234	Alzheimer's disease (AD) is a neurodegenerative disease that remarkably imposes a huge global public health burden.
32522544	4	43	theme	16S	550:552	arg1	sequencing					564:573	high-throughput 16S rRNA gene sequencing	534:573	high-throughput 16S rRNA gene sequencing	534:573	Here, gut microbiota and metabolites SCFAs were analyzed through high-throughput 16S rRNA gene sequencing and GC-MS, respectively.
32522544	9	44	theme	brain	1346:1350	arg1	IR					1372:1373	IR	1372:1373	IR	1372:1373	Moreover, M-β-Glu and S-β-Glu ameliorated neuroinflammation and brain insulin resistance (IR), which played a central role in the process of AD pathology.
32522544	9	44	theme	brain	1346:1350	arg1	resistance					1360:1369	brain insulin resistance	1346:1369	brain insulin resistance (IR)	1346:1374	Moreover, M-β-Glu and S-β-Glu ameliorated neuroinflammation and brain insulin resistance (IR), which played a central role in the process of AD pathology.
32522544	1	45	theme	global	208:213	arg1	burden					229:234	a huge global public health burden	201:234	a huge global public health burden	201:234	Alzheimer's disease (AD) is a neurodegenerative disease that remarkably imposes a huge global public health burden.
32522544	0	46	theme	gut	58:60	arg1	microbiota					62:71	gut microbiota	58:71	gut microbiota	58:71	Yeast β-glucan alleviates cognitive deficit by regulating gut microbiota and metabolites in Aβ1-42-induced AD-like mice.
32522544	4	47	theme	high-throughput	534:548	arg1	sequencing					564:573	high-throughput 16S rRNA gene sequencing	534:573	high-throughput 16S rRNA gene sequencing	534:573	Here, gut microbiota and metabolites SCFAs were analyzed through high-throughput 16S rRNA gene sequencing and GC-MS, respectively.
32522544	7	48	theme	regulated	1103:1111	arg1	microbiota					1117:1126	M-β-Glu regulated gut microbiota	1095:1126	M-β-Glu regulated gut microbiota	1095:1126	M-β-Glu regulated gut microbiota act as prebiotics better than S-β-Glu.
32522544	7	48	theme	regulated	1103:1111	arg1	prebiotics					1135:1144	prebiotics	1135:1144	prebiotics better than S-β-Glu	1135:1164	M-β-Glu regulated gut microbiota act as prebiotics better than S-β-Glu.
32522544	0	49	from	microbiota	62:71	arg1	mice					115:118	Aβ1-42-induced AD-like mice	92:118	Aβ1-42-induced AD-like mice	92:118	Yeast β-glucan alleviates cognitive deficit by regulating gut microbiota and metabolites in Aβ1-42-induced AD-like mice.
32522544	6	50	theme	yeast	799:803	arg1	M-β-Glu					815:821	M-β-Glu	815:821	M-β-Glu	815:821	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	6	50	theme	yeast	799:803	arg1	β-glucan					805:812	macro-molecular yeast β-glucan	783:812	macro-molecular yeast β-glucan (M-β-Glu)	783:822	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	4	51	theme	gene	559:562	arg1	sequencing					564:573	high-throughput 16S rRNA gene sequencing	534:573	high-throughput 16S rRNA gene sequencing	534:573	Here, gut microbiota and metabolites SCFAs were analyzed through high-throughput 16S rRNA gene sequencing and GC-MS, respectively.
32522544	7	52	theme	gut	1113:1115	arg1	microbiota					1117:1126	M-β-Glu regulated gut microbiota	1095:1126	M-β-Glu regulated gut microbiota	1095:1126	M-β-Glu regulated gut microbiota act as prebiotics better than S-β-Glu.
32522544	7	52	theme	gut	1113:1115	arg1	prebiotics					1135:1144	prebiotics	1135:1144	prebiotics better than S-β-Glu	1135:1164	M-β-Glu regulated gut microbiota act as prebiotics better than S-β-Glu.
32522544	6	53	theme	gut	880:882	arg1	microbiota					884:893	the gut microbiota	876:893	the gut microbiota	876:893	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	6	54	theme	macro-molecular	783:797	arg1	M-β-Glu					815:821	M-β-Glu	815:821	M-β-Glu	815:821	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	6	54	theme	macro-molecular	783:797	arg1	β-glucan					805:812	macro-molecular yeast β-glucan	783:812	macro-molecular yeast β-glucan (M-β-Glu)	783:822	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	4	55	theme	rRNA	554:557	arg1	sequencing					564:573	high-throughput 16S rRNA gene sequencing	534:573	high-throughput 16S rRNA gene sequencing	534:573	Here, gut microbiota and metabolites SCFAs were analyzed through high-throughput 16S rRNA gene sequencing and GC-MS, respectively.
32522544	2	56	used	used	300:303	arg2	β-glucans					243:251	Yeast β-glucans	237:251	Yeast β-glucans	237:251	Yeast β-glucans have been incorporated in functional foods and used in prophylactic applications owing to their biological effects.
32522544	1	57	theme	public	215:220	arg1	burden					229:234	a huge global public health burden	201:234	a huge global public health burden	201:234	Alzheimer's disease (AD) is a neurodegenerative disease that remarkably imposes a huge global public health burden.
32522544	7	58	theme	M-β-Glu	1095:1101	arg1	microbiota					1117:1126	M-β-Glu regulated gut microbiota	1095:1126	M-β-Glu regulated gut microbiota	1095:1126	M-β-Glu regulated gut microbiota act as prebiotics better than S-β-Glu.
32522544	7	58	theme	M-β-Glu	1095:1101	arg1	prebiotics					1135:1144	prebiotics	1135:1144	prebiotics better than S-β-Glu	1135:1164	M-β-Glu regulated gut microbiota act as prebiotics better than S-β-Glu.
32522544	1	59	theme	health	222:227	arg1	burden					229:234	a huge global public health burden	201:234	a huge global public health burden	201:234	Alzheimer's disease (AD) is a neurodegenerative disease that remarkably imposes a huge global public health burden.
32522544	6	60	theme	composition	861:871	arg1	alterations					842:852	evident alterations	834:852	evident alterations of the composition of the gut microbiota	834:893	Aβ1-42-induced AD mice treated with small-molecular yeast β-glucan (S-β-Glu) or macro-molecular yeast β-glucan (M-β-Glu) exhibited evident alterations of the composition of the gut microbiota, especially in some beneficial bacteria and inflammatory-related bacteria such as Lactobacillus, Bifidobacterium, Desulfovibrio, Oscillibacter, Mucispirillum, Alistipes, Anaerotruncus, and Rikenella.
32522544	8	61	theme	key	1205:1207	arg1	microbiota					1209:1218	the key microbiota	1201:1218	the key microbiota closely associated with AD-related pathologies and cognition	1201:1279	Correlation analysis demonstrated the key microbiota closely associated with AD-related pathologies and cognition.
32522544	2	62	theme	Yeast	237:241	arg1	β-glucans					243:251	Yeast β-glucans	237:251	Yeast β-glucans	237:251	Yeast β-glucans have been incorporated in functional foods and used in prophylactic applications owing to their biological effects.
33483043	2	0	dep	min	470:472	arg1	IT					474:475	IT	474:475	IT	474:475	The maximum extraction yield (32.81 %) was obtained under 20.94 mL/g WSR, 414.4 W power, and 12.75 min IT.
33483043	1	1	theme	microwave-assisted	269:286	arg1	extraction					288:297	microwave-assisted extraction	269:297	microwave-assisted extraction (MAE)	269:303	The effects of water to solids ratio (WSR, 10-30 mL/g), power (180-540 W), and irradiation time (IT, 5-15 min) in microwave-assisted extraction (MAE) were optimized to extract polysaccharides from melon peels (PMP).
33483043	1	1	theme	microwave-assisted	269:286	arg1	MAE					300:302	MAE	300:302	MAE	300:302	The effects of water to solids ratio (WSR, 10-30 mL/g), power (180-540 W), and irradiation time (IT, 5-15 min) in microwave-assisted extraction (MAE) were optimized to extract polysaccharides from melon peels (PMP).
33483043	1	2	theme	water	170:174	arg1	effects					159:165	The effects	155:165	The effects of water to solids ratio (WSR, 10-30 mL/g), power (180-540 W), and irradiation time (IT, 5-15 min) in microwave-assisted extraction (MAE)	155:303	The effects of water to solids ratio (WSR, 10-30 mL/g), power (180-540 W), and irradiation time (IT, 5-15 min) in microwave-assisted extraction (MAE) were optimized to extract polysaccharides from melon peels (PMP).
33483043	1	3	from	peels	358:362	arg1	polysaccharides					331:345	extract polysaccharides	323:345	extract polysaccharides from melon peels (PMP)	323:368	The effects of water to solids ratio (WSR, 10-30 mL/g), power (180-540 W), and irradiation time (IT, 5-15 min) in microwave-assisted extraction (MAE) were optimized to extract polysaccharides from melon peels (PMP).
33483043	4	4	theme	dose-dependent	655:668	arg1	behavior					698:705	An ascending dose-dependent antiradical and antioxidant behavior	642:705	An ascending dose-dependent antiradical and antioxidant behavior for PMP (0-5.0 mg/mL)	642:727	An ascending dose-dependent antiradical and antioxidant behavior for PMP (0-5.0 mg/mL) was found.
33483043	1	5	dep	time	246:249	arg1	IT					252:253	IT	252:253	IT	252:253	The effects of water to solids ratio (WSR, 10-30 mL/g), power (180-540 W), and irradiation time (IT, 5-15 min) in microwave-assisted extraction (MAE) were optimized to extract polysaccharides from melon peels (PMP).
33483043	1	5	dep	time	246:249	arg1	min					261:263	5-15 min	256:263	5-15 min	256:263	The effects of water to solids ratio (WSR, 10-30 mL/g), power (180-540 W), and irradiation time (IT, 5-15 min) in microwave-assisted extraction (MAE) were optimized to extract polysaccharides from melon peels (PMP).
33483043	2	6	theme	mL/g	435:438	arg1	WSR					440:442	20.94 mL/g WSR	429:442	20.94 mL/g WSR	429:442	The maximum extraction yield (32.81 %) was obtained under 20.94 mL/g WSR, 414.4 W power, and 12.75 min IT.
33483043	1	7	from	effects	159:165	arg1	extraction					288:297	microwave-assisted extraction	269:297	microwave-assisted extraction (MAE)	269:303	The effects of water to solids ratio (WSR, 10-30 mL/g), power (180-540 W), and irradiation time (IT, 5-15 min) in microwave-assisted extraction (MAE) were optimized to extract polysaccharides from melon peels (PMP).
33483043	1	7	from	effects	159:165	arg1	MAE					300:302	MAE	300:302	MAE	300:302	The effects of water to solids ratio (WSR, 10-30 mL/g), power (180-540 W), and irradiation time (IT, 5-15 min) in microwave-assisted extraction (MAE) were optimized to extract polysaccharides from melon peels (PMP).
33483043	5	8	theme	mixing	933:938	arg1	time					940:943	the mixing time	929:943	the mixing time (p < 0.05)	929:954	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	5	8	theme	mixing	933:938	arg1	<					948:948	p < 0.05	946:953	p < 0.05	946:953	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	6	9	theme	%	1050:1050	arg1	PMPs					1052:1055	5.0 % PMPs	1046:1055	5.0 % PMPs	1046:1055	The highest emulsifying activity index (44.1 m2/g) and emulsifying stability (69.3 %) at 5.0 % PMPs were determined.
33483043	7	10	theme	FTIR-identified	1088:1102	arg1	groups					1115:1120	FTIR-identified functional groups	1088:1120	FTIR-identified functional groups	1088:1120	PMP gels with FTIR-identified functional groups can be formulated in new gluten-free functional products.
33483043	4	11	theme	ascending	645:653	arg1	behavior					698:705	An ascending dose-dependent antiradical and antioxidant behavior	642:705	An ascending dose-dependent antiradical and antioxidant behavior for PMP (0-5.0 mg/mL)	642:727	An ascending dose-dependent antiradical and antioxidant behavior for PMP (0-5.0 mg/mL) was found.
33483043	1	12	theme	solids	179:184	arg1	WSR					193:195	WSR	193:195	WSR	193:195	The effects of water to solids ratio (WSR, 10-30 mL/g), power (180-540 W), and irradiation time (IT, 5-15 min) in microwave-assisted extraction (MAE) were optimized to extract polysaccharides from melon peels (PMP).
33483043	1	12	theme	solids	179:184	arg1	ratio					186:190	solids ratio	179:190	solids ratio (WSR, 10-30 mL/g)	179:208	The effects of water to solids ratio (WSR, 10-30 mL/g), power (180-540 W), and irradiation time (IT, 5-15 min) in microwave-assisted extraction (MAE) were optimized to extract polysaccharides from melon peels (PMP).
33483043	3	13	theme	d-galacturonic	586:599	arg1	acid					601:604	d-galacturonic acid	586:604	d-galacturonic acid	586:604	The main monosaccharide composition of purified PMP with an average molecular weight of 5.71 × 104 kDa were d-galacturonic acid, arabinose, glucose, and galactose.
33483043	3	13	theme	d-galacturonic	586:599	arg1	composition					502:512	The main monosaccharide composition	478:512	The main monosaccharide composition of purified PMP with an average molecular weight of 5.71 × 104 kDa	478:579	The main monosaccharide composition of purified PMP with an average molecular weight of 5.71 × 104 kDa were d-galacturonic acid, arabinose, glucose, and galactose.
33483043	4	14	theme	antioxidant	686:696	arg1	behavior					698:705	An ascending dose-dependent antiradical and antioxidant behavior	642:705	An ascending dose-dependent antiradical and antioxidant behavior for PMP (0-5.0 mg/mL)	642:727	An ascending dose-dependent antiradical and antioxidant behavior for PMP (0-5.0 mg/mL) was found.
33483043	6	15	theme	activity	981:988	arg1	index					990:994	The highest emulsifying activity index	957:994	The highest emulsifying activity index (44.1 m2/g)	957:1006	The highest emulsifying activity index (44.1 m2/g) and emulsifying stability (69.3 %) at 5.0 % PMPs were determined.
33483043	6	15	theme	activity	981:988	arg1	m2/g					1002:1005	44.1 m2/g	997:1005	44.1 m2/g	997:1005	The highest emulsifying activity index (44.1 m2/g) and emulsifying stability (69.3 %) at 5.0 % PMPs were determined.
33483043	2	16	theme	maximum	375:381	arg1	yield					394:398	The maximum extraction yield	371:398	The maximum extraction yield (32.81 %)	371:408	The maximum extraction yield (32.81 %) was obtained under 20.94 mL/g WSR, 414.4 W power, and 12.75 min IT.
33483043	2	16	theme	maximum	375:381	arg1	%					407:407	32.81 %	401:407	32.81 %	401:407	The maximum extraction yield (32.81 %) was obtained under 20.94 mL/g WSR, 414.4 W power, and 12.75 min IT.
33483043	6	17	theme	emulsifying	969:979	arg1	index					990:994	The highest emulsifying activity index	957:994	The highest emulsifying activity index (44.1 m2/g)	957:1006	The highest emulsifying activity index (44.1 m2/g) and emulsifying stability (69.3 %) at 5.0 % PMPs were determined.
33483043	6	17	theme	emulsifying	969:979	arg1	m2/g					1002:1005	44.1 m2/g	997:1005	44.1 m2/g	997:1005	The highest emulsifying activity index (44.1 m2/g) and emulsifying stability (69.3 %) at 5.0 % PMPs were determined.
33483043	7	18	theme	functional	1104:1113	arg1	groups					1115:1120	FTIR-identified functional groups	1088:1120	FTIR-identified functional groups	1088:1120	PMP gels with FTIR-identified functional groups can be formulated in new gluten-free functional products.
33483043	0	19	theme	Microwave-assisted	0:17	arg1	extraction					19:28	Microwave-assisted extraction	0:28	Microwave-assisted extraction of arabinan-rich pectic polysaccharides from melon peels: Optimization, purification, bioactivity, and techno-functionality.	0:153	Microwave-assisted extraction of arabinan-rich pectic polysaccharides from melon peels: Optimization, purification, bioactivity, and techno-functionality.
33483043	1	20	theme	irradiation	234:244	arg1	time					246:249	irradiation time	234:249	irradiation time (IT, 5-15 min)	234:264	The effects of water to solids ratio (WSR, 10-30 mL/g), power (180-540 W), and irradiation time (IT, 5-15 min) in microwave-assisted extraction (MAE) were optimized to extract polysaccharides from melon peels (PMP).
33483043	6	21	theme	highest	961:967	arg1	index					990:994	The highest emulsifying activity index	957:994	The highest emulsifying activity index (44.1 m2/g)	957:1006	The highest emulsifying activity index (44.1 m2/g) and emulsifying stability (69.3 %) at 5.0 % PMPs were determined.
33483043	6	21	theme	highest	961:967	arg1	m2/g					1002:1005	44.1 m2/g	997:1005	44.1 m2/g	997:1005	The highest emulsifying activity index (44.1 m2/g) and emulsifying stability (69.3 %) at 5.0 % PMPs were determined.
33483043	7	22	with	gels	1078:1081	arg1	groups					1115:1120	FTIR-identified functional groups	1088:1120	FTIR-identified functional groups	1088:1120	PMP gels with FTIR-identified functional groups can be formulated in new gluten-free functional products.
33483043	3	23	theme	average	538:544	arg1	weight					556:561	an average molecular weight	535:561	an average molecular weight of 5.71 × 104 kDa	535:579	The main monosaccharide composition of purified PMP with an average molecular weight of 5.71 × 104 kDa were d-galacturonic acid, arabinose, glucose, and galactose.
33483043	0	24	theme	arabinan-rich	33:45	arg1	polysaccharides					54:68	arabinan-rich pectic polysaccharides	33:68	arabinan-rich pectic polysaccharides	33:68	Microwave-assisted extraction of arabinan-rich pectic polysaccharides from melon peels: Optimization, purification, bioactivity, and techno-functionality.
33483043	3	25	theme	monosaccharide	487:500	arg1	composition					502:512	The main monosaccharide composition	478:512	The main monosaccharide composition of purified PMP with an average molecular weight of 5.71 × 104 kDa	478:579	The main monosaccharide composition of purified PMP with an average molecular weight of 5.71 × 104 kDa were d-galacturonic acid, arabinose, glucose, and galactose.
33483043	3	25	theme	monosaccharide	487:500	arg1	acid					601:604	d-galacturonic acid	586:604	d-galacturonic acid	586:604	The main monosaccharide composition of purified PMP with an average molecular weight of 5.71 × 104 kDa were d-galacturonic acid, arabinose, glucose, and galactose.
33483043	2	26	theme	W	451:451	arg1	power					453:457	414.4 W power	445:457	414.4 W power	445:457	The maximum extraction yield (32.81 %) was obtained under 20.94 mL/g WSR, 414.4 W power, and 12.75 min IT.
33483043	7	27	theme	gluten-free	1147:1157	arg1	products					1170:1177	new gluten-free functional products	1143:1177	new gluten-free functional products	1143:1177	PMP gels with FTIR-identified functional groups can be formulated in new gluten-free functional products.
33483043	0	28	theme	polysaccharides	54:68	arg1	extraction					19:28	Microwave-assisted extraction	0:28	Microwave-assisted extraction of arabinan-rich pectic polysaccharides from melon peels: Optimization, purification, bioactivity, and techno-functionality.	0:153	Microwave-assisted extraction of arabinan-rich pectic polysaccharides from melon peels: Optimization, purification, bioactivity, and techno-functionality.
33483043	1	29	theme	extract	323:329	arg1	polysaccharides					331:345	extract polysaccharides	323:345	extract polysaccharides from melon peels (PMP)	323:368	The effects of water to solids ratio (WSR, 10-30 mL/g), power (180-540 W), and irradiation time (IT, 5-15 min) in microwave-assisted extraction (MAE) were optimized to extract polysaccharides from melon peels (PMP).
33483043	5	30	theme	PMP	865:867	arg1	concentration					869:881	PMP concentration	865:881	PMP concentration	865:881	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	5	31	theme	initial	744:750	arg1	function					853:860	a function	851:860	a function of PMP concentration	851:881	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	5	31	theme	initial	744:750	arg1	stability					796:804	foaming stability	788:804	foaming stability (5.2-65.2 %)	788:817	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	5	31	theme	initial	744:750	arg1	capacity					760:767	The initial foaming capacity	740:767	The initial foaming capacity (38.6-110.3 %)	740:782	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	5	31	theme	initial	744:750	arg1	%					781:781	38.6-110.3 %	770:781	38.6-110.3 %	770:781	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	7	32	theme	PMP	1074:1076	arg1	gels					1078:1081	PMP gels	1074:1081	PMP gels with FTIR-identified functional groups	1074:1120	PMP gels with FTIR-identified functional groups can be formulated in new gluten-free functional products.
33483043	0	33	theme	pectic	47:52	arg1	polysaccharides					54:68	arabinan-rich pectic polysaccharides	33:68	arabinan-rich pectic polysaccharides	33:68	Microwave-assisted extraction of arabinan-rich pectic polysaccharides from melon peels: Optimization, purification, bioactivity, and techno-functionality.
33483043	3	34	theme	PMP	526:528	arg1	composition					502:512	The main monosaccharide composition	478:512	The main monosaccharide composition of purified PMP with an average molecular weight of 5.71 × 104 kDa	478:579	The main monosaccharide composition of purified PMP with an average molecular weight of 5.71 × 104 kDa were d-galacturonic acid, arabinose, glucose, and galactose.
33483043	3	34	theme	PMP	526:528	arg1	acid					601:604	d-galacturonic acid	586:604	d-galacturonic acid	586:604	The main monosaccharide composition of purified PMP with an average molecular weight of 5.71 × 104 kDa were d-galacturonic acid, arabinose, glucose, and galactose.
33483043	1	35	dep	WSR	193:195	arg1	mL/g					204:207	10-30 mL/g	198:207	10-30 mL/g	198:207	The effects of water to solids ratio (WSR, 10-30 mL/g), power (180-540 W), and irradiation time (IT, 5-15 min) in microwave-assisted extraction (MAE) were optimized to extract polysaccharides from melon peels (PMP).
33483043	0	36	theme	melon	75:79	arg1	peels					81:85	melon peels	75:85	melon peels	75:85	Microwave-assisted extraction of arabinan-rich pectic polysaccharides from melon peels: Optimization, purification, bioactivity, and techno-functionality.
33483043	7	37	theme	new	1143:1145	arg1	products					1170:1177	new gluten-free functional products	1143:1177	new gluten-free functional products	1143:1177	PMP gels with FTIR-identified functional groups can be formulated in new gluten-free functional products.
33483043	0	38	from	peels	81:85	arg1	extraction					19:28	Microwave-assisted extraction	0:28	Microwave-assisted extraction of arabinan-rich pectic polysaccharides from melon peels: Optimization, purification, bioactivity, and techno-functionality.	0:153	Microwave-assisted extraction of arabinan-rich pectic polysaccharides from melon peels: Optimization, purification, bioactivity, and techno-functionality.
33483043	7	39	theme	functional	1159:1168	arg1	products					1170:1177	new gluten-free functional products	1143:1177	new gluten-free functional products	1143:1177	PMP gels with FTIR-identified functional groups can be formulated in new gluten-free functional products.
33483043	2	40	theme	extraction	383:392	arg1	yield					394:398	The maximum extraction yield	371:398	The maximum extraction yield (32.81 %)	371:408	The maximum extraction yield (32.81 %) was obtained under 20.94 mL/g WSR, 414.4 W power, and 12.75 min IT.
33483043	2	40	theme	extraction	383:392	arg1	%					407:407	32.81 %	401:407	32.81 %	401:407	The maximum extraction yield (32.81 %) was obtained under 20.94 mL/g WSR, 414.4 W power, and 12.75 min IT.
33483043	5	41	theme	p	946:946	arg1	time					940:943	the mixing time	929:943	the mixing time (p < 0.05)	929:954	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	5	41	theme	p	946:946	arg1	<					948:948	p < 0.05	946:953	p < 0.05	946:953	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	1	42	theme	melon	352:356	arg1	peels					358:362	melon peels	352:362	melon peels (PMP)	352:368	The effects of water to solids ratio (WSR, 10-30 mL/g), power (180-540 W), and irradiation time (IT, 5-15 min) in microwave-assisted extraction (MAE) were optimized to extract polysaccharides from melon peels (PMP).
33483043	1	42	theme	melon	352:356	arg1	PMP					365:367	PMP	365:367	PMP	365:367	The effects of water to solids ratio (WSR, 10-30 mL/g), power (180-540 W), and irradiation time (IT, 5-15 min) in microwave-assisted extraction (MAE) were optimized to extract polysaccharides from melon peels (PMP).
33483043	6	43	theme	5.0	1046:1048	arg1	%					1050:1050	%	1050:1050	%	1050:1050	The highest emulsifying activity index (44.1 m2/g) and emulsifying stability (69.3 %) at 5.0 % PMPs were determined.
33483043	5	44	dep	increased	838:846	arg1	%					892:892	1.0-5.0 %	884:892	1.0-5.0 %	884:892	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	5	45	theme	concentration	869:881	arg1	function					853:860	a function	851:860	a function of PMP concentration	851:881	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	5	45	theme	concentration	869:881	arg1	stability					796:804	foaming stability	788:804	foaming stability (5.2-65.2 %)	788:817	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	5	45	theme	concentration	869:881	arg1	capacity					760:767	The initial foaming capacity	740:767	The initial foaming capacity (38.6-110.3 %)	740:782	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	6	46	theme	emulsifying	1012:1022	arg1	stability					1024:1032	emulsifying stability	1012:1032	emulsifying stability (69.3 %)	1012:1041	The highest emulsifying activity index (44.1 m2/g) and emulsifying stability (69.3 %) at 5.0 % PMPs were determined.
33483043	6	46	theme	emulsifying	1012:1022	arg1	%					1040:1040	69.3 %	1035:1040	69.3 %	1035:1040	The highest emulsifying activity index (44.1 m2/g) and emulsifying stability (69.3 %) at 5.0 % PMPs were determined.
33483043	4	47	theme	antiradical	670:680	arg1	behavior					698:705	An ascending dose-dependent antiradical and antioxidant behavior	642:705	An ascending dose-dependent antiradical and antioxidant behavior for PMP (0-5.0 mg/mL)	642:727	An ascending dose-dependent antiradical and antioxidant behavior for PMP (0-5.0 mg/mL) was found.
33483043	3	48	theme	main	482:485	arg1	composition					502:512	The main monosaccharide composition	478:512	The main monosaccharide composition of purified PMP with an average molecular weight of 5.71 × 104 kDa	478:579	The main monosaccharide composition of purified PMP with an average molecular weight of 5.71 × 104 kDa were d-galacturonic acid, arabinose, glucose, and galactose.
33483043	3	48	theme	main	482:485	arg1	acid					601:604	d-galacturonic acid	586:604	d-galacturonic acid	586:604	The main monosaccharide composition of purified PMP with an average molecular weight of 5.71 × 104 kDa were d-galacturonic acid, arabinose, glucose, and galactose.
33483043	3	49	theme	molecular	546:554	arg1	weight					556:561	an average molecular weight	535:561	an average molecular weight of 5.71 × 104 kDa	535:579	The main monosaccharide composition of purified PMP with an average molecular weight of 5.71 × 104 kDa were d-galacturonic acid, arabinose, glucose, and galactose.
33483043	3	50	theme	purified	517:524	arg1	PMP					526:528	purified PMP	517:528	purified PMP	517:528	The main monosaccharide composition of purified PMP with an average molecular weight of 5.71 × 104 kDa were d-galacturonic acid, arabinose, glucose, and galactose.
33483043	5	51	theme	foaming	788:794	arg1	function					853:860	a function	851:860	a function of PMP concentration	851:881	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	5	51	theme	foaming	788:794	arg1	stability					796:804	foaming stability	788:804	foaming stability (5.2-65.2 %)	788:817	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	5	51	theme	foaming	788:794	arg1	%					816:816	5.2-65.2 %	807:816	5.2-65.2 %	807:816	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	5	51	theme	foaming	788:794	arg1	capacity					760:767	The initial foaming capacity	740:767	The initial foaming capacity (38.6-110.3 %)	740:782	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	6	52	from	PMPs	1052:1055	arg1	index					990:994	The highest emulsifying activity index	957:994	The highest emulsifying activity index (44.1 m2/g)	957:1006	The highest emulsifying activity index (44.1 m2/g) and emulsifying stability (69.3 %) at 5.0 % PMPs were determined.
33483043	6	52	from	PMPs	1052:1055	arg1	m2/g					1002:1005	44.1 m2/g	997:1005	44.1 m2/g	997:1005	The highest emulsifying activity index (44.1 m2/g) and emulsifying stability (69.3 %) at 5.0 % PMPs were determined.
33483043	6	52	from	PMPs	1052:1055	arg1	stability					1024:1032	emulsifying stability	1012:1032	emulsifying stability (69.3 %)	1012:1041	The highest emulsifying activity index (44.1 m2/g) and emulsifying stability (69.3 %) at 5.0 % PMPs were determined.
33483043	6	52	from	PMPs	1052:1055	arg1	%					1040:1040	69.3 %	1035:1040	69.3 %	1035:1040	The highest emulsifying activity index (44.1 m2/g) and emulsifying stability (69.3 %) at 5.0 % PMPs were determined.
33483043	0	53	dep	extraction	19:28	arg1	Optimization					88:99	Optimization	88:99	Optimization	88:99	Microwave-assisted extraction of arabinan-rich pectic polysaccharides from melon peels: Optimization, purification, bioactivity, and techno-functionality.
33483043	0	53	dep	extraction	19:28	arg1	bioactivity					116:126	bioactivity	116:126	bioactivity	116:126	Microwave-assisted extraction of arabinan-rich pectic polysaccharides from melon peels: Optimization, purification, bioactivity, and techno-functionality.
33483043	0	53	dep	extraction	19:28	arg1	techno-functionality					133:152	techno-functionality	133:152	techno-functionality	133:152	Microwave-assisted extraction of arabinan-rich pectic polysaccharides from melon peels: Optimization, purification, bioactivity, and techno-functionality.
33483043	0	53	dep	extraction	19:28	arg1	purification					102:113	purification	102:113	purification	102:113	Microwave-assisted extraction of arabinan-rich pectic polysaccharides from melon peels: Optimization, purification, bioactivity, and techno-functionality.
33483043	5	54	theme	foaming	752:758	arg1	function					853:860	a function	851:860	a function of PMP concentration	851:881	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	5	54	theme	foaming	752:758	arg1	stability					796:804	foaming stability	788:804	foaming stability (5.2-65.2 %)	788:817	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	5	54	theme	foaming	752:758	arg1	capacity					760:767	The initial foaming capacity	740:767	The initial foaming capacity (38.6-110.3 %)	740:782	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	5	54	theme	foaming	752:758	arg1	%					781:781	38.6-110.3 %	770:781	38.6-110.3 %	770:781	The initial foaming capacity (38.6-110.3 %) and foaming stability (5.2-65.2 %) were significantly increased as a function of PMP concentration (1.0-5.0 %), while they reduced by increasing the mixing time (p < 0.05).
33483043	3	55	with	composition	502:512	arg1	weight					556:561	an average molecular weight	535:561	an average molecular weight of 5.71 × 104 kDa	535:579	The main monosaccharide composition of purified PMP with an average molecular weight of 5.71 × 104 kDa were d-galacturonic acid, arabinose, glucose, and galactose.
33483043	3	56	theme	kDa	577:579	arg1	weight					556:561	an average molecular weight	535:561	an average molecular weight of 5.71 × 104 kDa	535:579	The main monosaccharide composition of purified PMP with an average molecular weight of 5.71 × 104 kDa were d-galacturonic acid, arabinose, glucose, and galactose.
34560149	5	0	theme	chemical	771:778	arg1	composition					780:790	Their chemical composition	765:790	Their chemical composition	765:790	Their chemical composition differed in sugar, protein and uronic acid contents, and they were composed of six constituent monosaccharides with different ratios, with the average molecular weights of 1.12 × 103, 1.89 × 103, 1.41 × 103 and 2.02 × 103 kDa, respectively.
34560149	1	1	theme	Grifola	333:339	arg1	frondosa					341:348	Grifola frondosa	333:348	Grifola frondosa	333:348	A strategy by exogenous addition of quorum sensing molecule farnesol to improve the production, antioxidant activity and antitumor activity of extracellular polysaccharide (EPS) of Grifola frondosa by liquid fermentation was proposed in the study.
34560149	10	2	contain	had	1667:1669	arg1	adjustment					1634:1643	appropriate adjustment	1622:1643	appropriate adjustment of culture conditions	1622:1665	These results demonstrated that appropriate adjustment of culture conditions had potential application in the development of polysaccharides with high antioxidant and antitumor activity.
34560149	10	2	contain	had	1667:1669	arg2	application					1681:1691	potential application	1671:1691	potential application in the development of polysaccharides with high antioxidant and antitumor activity	1671:1774	These results demonstrated that appropriate adjustment of culture conditions had potential application in the development of polysaccharides with high antioxidant and antitumor activity.
34560149	11	3	theme	quorum	1902:1907	arg1	sensing					1909:1915	quorum sensing	1902:1915	quorum sensing molecules	1902:1925	It provided a new strategy to enhance the production and bioactivity of edible and medicinal fungal polysaccharides by using quorum sensing molecules.
34560149	8	4	theme	HeLa	1324:1327	arg1	cells					1330:1334	human cervical cancer (HeLa) cells	1301:1334	human cervical cancer (HeLa) cells	1301:1334	Antitumor experiments showed that they had strong inhibitory effects on human cervical cancer (HeLa) cells and human liver cancer cells (HepG2) cells.
34560149	1	5	theme	frondosa	341:348	arg1	EPS					325:327	EPS	325:327	EPS	325:327	A strategy by exogenous addition of quorum sensing molecule farnesol to improve the production, antioxidant activity and antitumor activity of extracellular polysaccharide (EPS) of Grifola frondosa by liquid fermentation was proposed in the study.
34560149	1	5	theme	frondosa	341:348	arg1	polysaccharide					309:322	extracellular polysaccharide	295:322	extracellular polysaccharide (EPS) of Grifola frondosa	295:348	A strategy by exogenous addition of quorum sensing molecule farnesol to improve the production, antioxidant activity and antitumor activity of extracellular polysaccharide (EPS) of Grifola frondosa by liquid fermentation was proposed in the study.
34560149	8	6	theme	Antitumor	1229:1237	arg1	experiments					1239:1249	Antitumor experiments	1229:1249	Antitumor experiments	1229:1249	Antitumor experiments showed that they had strong inhibitory effects on human cervical cancer (HeLa) cells and human liver cancer cells (HepG2) cells.
34560149	0	7	dep	Grifola	111:117	arg1	frondosa					119:126	Grifola frondosa	111:126	Grifola frondosa	111:126	The effects of quorum sensing molecule farnesol on the yield and activity of extracellular polysaccharide from Grifola frondosa in liquid fermentation.
34560149	7	8	contain	had	1146:1148	arg1	they					1141:1144	they	1141:1144	they	1141:1144	Antioxidant experiments showed that they had strong scavenging activities on ABTS+, hydroxyl radical, O2- and DPPH radical.
34560149	7	8	contain	had	1146:1148	arg2	activities					1168:1177	strong scavenging activities	1150:1177	strong scavenging activities	1150:1177	Antioxidant experiments showed that they had strong scavenging activities on ABTS+, hydroxyl radical, O2- and DPPH radical.
34560149	1	9	theme	antioxidant	248:258	arg1	activity					260:267	antioxidant activity	248:267	antioxidant activity	248:267	A strategy by exogenous addition of quorum sensing molecule farnesol to improve the production, antioxidant activity and antitumor activity of extracellular polysaccharide (EPS) of Grifola frondosa by liquid fermentation was proposed in the study.
34560149	0	10	theme	extracellular	77:89	arg1	polysaccharide					91:104	extracellular polysaccharide	77:104	extracellular polysaccharide from Grifola frondosa	77:126	The effects of quorum sensing molecule farnesol on the yield and activity of extracellular polysaccharide from Grifola frondosa in liquid fermentation.
34560149	9	11	theme	biological	1524:1533	arg1	activity					1535:1542	the biological activity	1520:1542	the biological activity of EPS	1520:1549	Among the four polysaccharides, EPS-F-0.2M showed the highest antioxidant and antitumor activities, indicating that farnesol could regulate the biological activity of EPS by affecting structure and properties.
34560149	10	12	theme	antitumor	1757:1765	arg1	activity					1767:1774	antitumor activity	1757:1774	antitumor activity	1757:1774	These results demonstrated that appropriate adjustment of culture conditions had potential application in the development of polysaccharides with high antioxidant and antitumor activity.
34560149	8	13	theme	liver	1346:1350	arg1	cells					1373:1377	human liver cancer cells (HepG2) cells	1340:1377	human liver cancer cells (HepG2) cells	1340:1377	Antitumor experiments showed that they had strong inhibitory effects on human cervical cancer (HeLa) cells and human liver cancer cells (HepG2) cells.
34560149	6	14	theme	similar	1048:1054	arg1	spectra					1062:1068	similar FT-IR spectra	1048:1068	similar FT-IR spectra	1048:1068	They presented similar FT-IR spectra, but different surface morphology.
34560149	7	15	theme	DPPH	1215:1218	arg1	ABTS+					1182:1186	ABTS+	1182:1186	ABTS+	1182:1186	Antioxidant experiments showed that they had strong scavenging activities on ABTS+, hydroxyl radical, O2- and DPPH radical.
34560149	7	15	theme	DPPH	1215:1218	arg1	radical					1220:1226	DPPH radical	1215:1226	DPPH radical	1215:1226	Antioxidant experiments showed that they had strong scavenging activities on ABTS+, hydroxyl radical, O2- and DPPH radical.
34560149	1	16	theme	liquid	353:358	arg1	fermentation					360:371	liquid fermentation	353:371	liquid fermentation	353:371	A strategy by exogenous addition of quorum sensing molecule farnesol to improve the production, antioxidant activity and antitumor activity of extracellular polysaccharide (EPS) of Grifola frondosa by liquid fermentation was proposed in the study.
34560149	5	17	with	monosaccharides	887:901	arg1	ratios					918:923	different ratios	908:923	different ratios	908:923	Their chemical composition differed in sugar, protein and uronic acid contents, and they were composed of six constituent monosaccharides with different ratios, with the average molecular weights of 1.12 × 103, 1.89 × 103, 1.41 × 103 and 2.02 × 103 kDa, respectively.
34560149	8	18	theme	human	1340:1344	arg1	cells					1373:1377	human liver cancer cells (HepG2) cells	1340:1377	human liver cancer cells (HepG2) cells	1340:1377	Antitumor experiments showed that they had strong inhibitory effects on human cervical cancer (HeLa) cells and human liver cancer cells (HepG2) cells.
34560149	9	19	theme	antitumor	1458:1466	arg1	activities					1468:1477	antioxidant and antitumor activities	1442:1477	the highest antioxidant and antitumor activities	1430:1477	Among the four polysaccharides, EPS-F-0.2M showed the highest antioxidant and antitumor activities, indicating that farnesol could regulate the biological activity of EPS by affecting structure and properties.
34560149	11	20	theme	sensing	1909:1915	arg1	molecules					1917:1925	quorum sensing molecules	1902:1925	quorum sensing molecules	1902:1925	It provided a new strategy to enhance the production and bioactivity of edible and medicinal fungal polysaccharides by using quorum sensing molecules.
34560149	10	21	theme	appropriate	1622:1632	arg1	adjustment					1634:1643	appropriate adjustment	1622:1643	appropriate adjustment of culture conditions	1622:1665	These results demonstrated that appropriate adjustment of culture conditions had potential application in the development of polysaccharides with high antioxidant and antitumor activity.
34560149	8	22	theme	cervical	1307:1314	arg1	cells					1330:1334	human cervical cancer (HeLa) cells	1301:1334	human cervical cancer (HeLa) cells	1301:1334	Antitumor experiments showed that they had strong inhibitory effects on human cervical cancer (HeLa) cells and human liver cancer cells (HepG2) cells.
34560149	7	23	theme	Antioxidant	1105:1115	arg1	experiments					1117:1127	Antioxidant experiments	1105:1127	Antioxidant experiments	1105:1127	Antioxidant experiments showed that they had strong scavenging activities on ABTS+, hydroxyl radical, O2- and DPPH radical.
34560149	6	24	theme	FT-IR	1056:1060	arg1	spectra					1062:1068	similar FT-IR spectra	1048:1068	similar FT-IR spectra	1048:1068	They presented similar FT-IR spectra, but different surface morphology.
34560149	8	25	theme	cells	1359:1363	arg1	cells					1373:1377	human liver cancer cells (HepG2) cells	1340:1377	human liver cancer cells (HepG2) cells	1340:1377	Antitumor experiments showed that they had strong inhibitory effects on human cervical cancer (HeLa) cells and human liver cancer cells (HepG2) cells.
34560149	10	26	theme	potential	1671:1679	arg1	application					1681:1691	potential application	1671:1691	potential application in the development of polysaccharides with high antioxidant and antitumor activity	1671:1774	These results demonstrated that appropriate adjustment of culture conditions had potential application in the development of polysaccharides with high antioxidant and antitumor activity.
34560149	6	27	theme	surface	1085:1091	arg1	morphology					1093:1102	different surface morphology	1075:1102	different surface morphology	1075:1102	They presented similar FT-IR spectra, but different surface morphology.
34560149	0	28	theme	polysaccharide	91:104	arg1	activity					65:72	activity	65:72	activity	65:72	The effects of quorum sensing molecule farnesol on the yield and activity of extracellular polysaccharide from Grifola frondosa in liquid fermentation.
34560149	0	28	theme	polysaccharide	91:104	arg1	yield					55:59	yield	55:59	yield	55:59	The effects of quorum sensing molecule farnesol on the yield and activity of extracellular polysaccharide from Grifola frondosa in liquid fermentation.
34560149	5	29	theme	different	908:916	arg1	ratios					918:923	different ratios	908:923	different ratios	908:923	Their chemical composition differed in sugar, protein and uronic acid contents, and they were composed of six constituent monosaccharides with different ratios, with the average molecular weights of 1.12 × 103, 1.89 × 103, 1.41 × 103 and 2.02 × 103 kDa, respectively.
34560149	8	30	contain	had	1268:1270	arg1	they					1263:1266	they	1263:1266	they	1263:1266	Antitumor experiments showed that they had strong inhibitory effects on human cervical cancer (HeLa) cells and human liver cancer cells (HepG2) cells.
34560149	8	30	contain	had	1268:1270	arg2	effects					1290:1296	strong inhibitory effects	1272:1296	strong inhibitory effects	1272:1296	Antitumor experiments showed that they had strong inhibitory effects on human cervical cancer (HeLa) cells and human liver cancer cells (HepG2) cells.
34560149	8	31	theme	cancer	1352:1357	arg1	cells					1373:1377	human liver cancer cells (HepG2) cells	1340:1377	human liver cancer cells (HepG2) cells	1340:1377	Antitumor experiments showed that they had strong inhibitory effects on human cervical cancer (HeLa) cells and human liver cancer cells (HepG2) cells.
34560149	1	32	theme	exogenous	166:174	arg1	addition					176:183	exogenous addition	166:183	exogenous addition of quorum sensing molecule farnesol to improve the production, antioxidant activity and antitumor activity of extracellular polysaccharide (EPS) of Grifola frondosa by liquid fermentation	166:371	A strategy by exogenous addition of quorum sensing molecule farnesol to improve the production, antioxidant activity and antitumor activity of extracellular polysaccharide (EPS) of Grifola frondosa by liquid fermentation was proposed in the study.
34560149	5	33	theme	protein	811:817	arg1	contents					835:842	sugar, protein and uronic acid contents	804:842	sugar, protein and uronic acid contents	804:842	Their chemical composition differed in sugar, protein and uronic acid contents, and they were composed of six constituent monosaccharides with different ratios, with the average molecular weights of 1.12 × 103, 1.89 × 103, 1.41 × 103 and 2.02 × 103 kDa, respectively.
34560149	11	34	theme	fungal	1870:1875	arg1	polysaccharides					1877:1891	edible and medicinal fungal polysaccharides	1849:1891	edible and medicinal fungal polysaccharides	1849:1891	It provided a new strategy to enhance the production and bioactivity of edible and medicinal fungal polysaccharides by using quorum sensing molecules.
34560149	5	35	theme	average	935:941	arg1	weights					953:959	the average molecular weights	931:959	the average molecular weights of 1.12 × 103, 1.89 × 103, 1.41 × 103 and 2.02 × 103 kDa	931:1016	Their chemical composition differed in sugar, protein and uronic acid contents, and they were composed of six constituent monosaccharides with different ratios, with the average molecular weights of 1.12 × 103, 1.89 × 103, 1.41 × 103 and 2.02 × 103 kDa, respectively.
34560149	9	36	theme	antioxidant	1442:1452	arg1	activities					1468:1477	antioxidant and antitumor activities	1442:1477	the highest antioxidant and antitumor activities	1430:1477	Among the four polysaccharides, EPS-F-0.2M showed the highest antioxidant and antitumor activities, indicating that farnesol could regulate the biological activity of EPS by affecting structure and properties.
34560149	1	37	theme	antitumor	273:281	arg1	activity					283:290	antitumor activity	273:290	antitumor activity	273:290	A strategy by exogenous addition of quorum sensing molecule farnesol to improve the production, antioxidant activity and antitumor activity of extracellular polysaccharide (EPS) of Grifola frondosa by liquid fermentation was proposed in the study.
34560149	4	38	theme	antitumor	730:738	arg1	activities					740:749	antitumor activities	730:749	antitumor activities	730:749	The physicochemical properties, antioxidant activities and antitumor activities were studied.
34560149	8	39	theme	strong	1272:1277	arg1	effects					1290:1296	strong inhibitory effects	1272:1296	strong inhibitory effects	1272:1296	Antitumor experiments showed that they had strong inhibitory effects on human cervical cancer (HeLa) cells and human liver cancer cells (HepG2) cells.
34560149	0	40	theme	quorum	15:20	arg1	effects					4:10	The effects	0:10	The effects of quorum sensing molecule farnesol on the yield and activity of extracellular polysaccharide from Grifola frondosa in liquid fermentation.	0:150	The effects of quorum sensing molecule farnesol on the yield and activity of extracellular polysaccharide from Grifola frondosa in liquid fermentation.
34560149	10	41	theme	polysaccharides	1715:1729	arg1	development					1700:1710	the development	1696:1710	the development of polysaccharides with high antioxidant and antitumor activity	1696:1774	These results demonstrated that appropriate adjustment of culture conditions had potential application in the development of polysaccharides with high antioxidant and antitumor activity.
34560149	0	42	dep	yield	55:59	arg1	the					51:53	the	51:53	the	51:53	The effects of quorum sensing molecule farnesol on the yield and activity of extracellular polysaccharide from Grifola frondosa in liquid fermentation.
34560149	11	43	theme	polysaccharides	1877:1891	arg1	bioactivity					1834:1844	bioactivity	1834:1844	bioactivity	1834:1844	It provided a new strategy to enhance the production and bioactivity of edible and medicinal fungal polysaccharides by using quorum sensing molecules.
34560149	11	43	theme	polysaccharides	1877:1891	arg1	production					1819:1828	production	1819:1828	production	1819:1828	It provided a new strategy to enhance the production and bioactivity of edible and medicinal fungal polysaccharides by using quorum sensing molecules.
34560149	1	44	theme	quorum	188:193	arg1	sensing					195:201	quorum sensing	188:201	quorum sensing	188:201	A strategy by exogenous addition of quorum sensing molecule farnesol to improve the production, antioxidant activity and antitumor activity of extracellular polysaccharide (EPS) of Grifola frondosa by liquid fermentation was proposed in the study.
34560149	2	45	theme	EPS	421:423	arg1	1.25 g/L					449:456	1.25 g/L	449:456	1.25 g/L	449:456	The highest yield of EPS induced by farnesol was 1.25 g/L, which was 150% higher than that of the control.
34560149	2	45	theme	EPS	421:423	arg1	yield					412:416	The highest yield	400:416	The highest yield of EPS induced by farnesol	400:443	The highest yield of EPS induced by farnesol was 1.25 g/L, which was 150% higher than that of the control.
34560149	5	46	theme	molecular	943:951	arg1	weights					953:959	the average molecular weights	931:959	the average molecular weights of 1.12 × 103, 1.89 × 103, 1.41 × 103 and 2.02 × 103 kDa	931:1016	Their chemical composition differed in sugar, protein and uronic acid contents, and they were composed of six constituent monosaccharides with different ratios, with the average molecular weights of 1.12 × 103, 1.89 × 103, 1.41 × 103 and 2.02 × 103 kDa, respectively.
34560149	0	47	from	activity	65:72	arg1	Grifola					111:117	Grifola	111:117	Grifola	111:117	The effects of quorum sensing molecule farnesol on the yield and activity of extracellular polysaccharide from Grifola frondosa in liquid fermentation.
34560149	0	47	from	activity	65:72	arg1	fermentation					138:149	liquid fermentation	131:149	liquid fermentation	131:149	The effects of quorum sensing molecule farnesol on the yield and activity of extracellular polysaccharide from Grifola frondosa in liquid fermentation.
34560149	4	48	theme	physicochemical	675:689	arg1	properties					691:700	The physicochemical properties	671:700	The physicochemical properties	671:700	The physicochemical properties, antioxidant activities and antitumor activities were studied.
34560149	1	49	theme	sensing	195:201	arg1	addition					176:183	exogenous addition	166:183	exogenous addition of quorum sensing molecule farnesol to improve the production, antioxidant activity and antitumor activity of extracellular polysaccharide (EPS) of Grifola frondosa by liquid fermentation	166:371	A strategy by exogenous addition of quorum sensing molecule farnesol to improve the production, antioxidant activity and antitumor activity of extracellular polysaccharide (EPS) of Grifola frondosa by liquid fermentation was proposed in the study.
34560149	0	50	theme	molecule	30:37	arg1	farnesol					39:46	molecule farnesol	30:46	molecule farnesol	30:46	The effects of quorum sensing molecule farnesol on the yield and activity of extracellular polysaccharide from Grifola frondosa in liquid fermentation.
34560149	1	51	theme	extracellular	295:307	arg1	EPS					325:327	EPS	325:327	EPS	325:327	A strategy by exogenous addition of quorum sensing molecule farnesol to improve the production, antioxidant activity and antitumor activity of extracellular polysaccharide (EPS) of Grifola frondosa by liquid fermentation was proposed in the study.
34560149	1	51	theme	extracellular	295:307	arg1	polysaccharide					309:322	extracellular polysaccharide	295:322	extracellular polysaccharide (EPS) of Grifola frondosa	295:348	A strategy by exogenous addition of quorum sensing molecule farnesol to improve the production, antioxidant activity and antitumor activity of extracellular polysaccharide (EPS) of Grifola frondosa by liquid fermentation was proposed in the study.
34560149	0	52	theme	liquid	131:136	arg1	fermentation					138:149	liquid fermentation	131:149	liquid fermentation	131:149	The effects of quorum sensing molecule farnesol on the yield and activity of extracellular polysaccharide from Grifola frondosa in liquid fermentation.
34560149	2	53	theme	highest	404:410	arg1	1.25 g/L					449:456	1.25 g/L	449:456	1.25 g/L	449:456	The highest yield of EPS induced by farnesol was 1.25 g/L, which was 150% higher than that of the control.
34560149	2	53	theme	highest	404:410	arg1	yield					412:416	The highest yield	400:416	The highest yield of EPS induced by farnesol	400:443	The highest yield of EPS induced by farnesol was 1.25 g/L, which was 150% higher than that of the control.
34560149	1	54	theme	molecule	203:210	arg1	farnesol					212:219	molecule farnesol	203:219	molecule farnesol	203:219	A strategy by exogenous addition of quorum sensing molecule farnesol to improve the production, antioxidant activity and antitumor activity of extracellular polysaccharide (EPS) of Grifola frondosa by liquid fermentation was proposed in the study.
34560149	10	55	theme	culture	1648:1654	arg1	conditions					1656:1665	culture conditions	1648:1665	culture conditions	1648:1665	These results demonstrated that appropriate adjustment of culture conditions had potential application in the development of polysaccharides with high antioxidant and antitumor activity.
34560149	1	56	theme	polysaccharide	309:322	arg1	production					236:245	the production	232:245	the production	232:245	A strategy by exogenous addition of quorum sensing molecule farnesol to improve the production, antioxidant activity and antitumor activity of extracellular polysaccharide (EPS) of Grifola frondosa by liquid fermentation was proposed in the study.
34560149	1	56	theme	polysaccharide	309:322	arg1	activity					260:267	antioxidant activity	248:267	antioxidant activity	248:267	A strategy by exogenous addition of quorum sensing molecule farnesol to improve the production, antioxidant activity and antitumor activity of extracellular polysaccharide (EPS) of Grifola frondosa by liquid fermentation was proposed in the study.
34560149	1	56	theme	polysaccharide	309:322	arg1	activity					283:290	antitumor activity	273:290	antitumor activity	273:290	A strategy by exogenous addition of quorum sensing molecule farnesol to improve the production, antioxidant activity and antitumor activity of extracellular polysaccharide (EPS) of Grifola frondosa by liquid fermentation was proposed in the study.
34560149	0	57	from	yield	55:59	arg1	Grifola					111:117	Grifola	111:117	Grifola	111:117	The effects of quorum sensing molecule farnesol on the yield and activity of extracellular polysaccharide from Grifola frondosa in liquid fermentation.
34560149	0	57	from	yield	55:59	arg1	fermentation					138:149	liquid fermentation	131:149	liquid fermentation	131:149	The effects of quorum sensing molecule farnesol on the yield and activity of extracellular polysaccharide from Grifola frondosa in liquid fermentation.
34560149	5	58	theme	uronic	823:828	arg1	acid					830:833	uronic acid	823:833	uronic acid	823:833	Their chemical composition differed in sugar, protein and uronic acid contents, and they were composed of six constituent monosaccharides with different ratios, with the average molecular weights of 1.12 × 103, 1.89 × 103, 1.41 × 103 and 2.02 × 103 kDa, respectively.
34560149	11	59	theme	medicinal	1860:1868	arg1	polysaccharides					1877:1891	edible and medicinal fungal polysaccharides	1849:1891	edible and medicinal fungal polysaccharides	1849:1891	It provided a new strategy to enhance the production and bioactivity of edible and medicinal fungal polysaccharides by using quorum sensing molecules.
34560149	5	60	theme	sugar	804:808	arg1	contents					835:842	sugar, protein and uronic acid contents	804:842	sugar, protein and uronic acid contents	804:842	Their chemical composition differed in sugar, protein and uronic acid contents, and they were composed of six constituent monosaccharides with different ratios, with the average molecular weights of 1.12 × 103, 1.89 × 103, 1.41 × 103 and 2.02 × 103 kDa, respectively.
34560149	9	61	theme	highest	1434:1440	arg1	activities					1468:1477	antioxidant and antitumor activities	1442:1477	the highest antioxidant and antitumor activities	1430:1477	Among the four polysaccharides, EPS-F-0.2M showed the highest antioxidant and antitumor activities, indicating that farnesol could regulate the biological activity of EPS by affecting structure and properties.
34560149	8	62	theme	HepG2	1366:1370	arg1	cells					1373:1377	human liver cancer cells (HepG2) cells	1340:1377	human liver cancer cells (HepG2) cells	1340:1377	Antitumor experiments showed that they had strong inhibitory effects on human cervical cancer (HeLa) cells and human liver cancer cells (HepG2) cells.
34560149	11	63	theme	edible	1849:1854	arg1	polysaccharides					1877:1891	edible and medicinal fungal polysaccharides	1849:1891	edible and medicinal fungal polysaccharides	1849:1891	It provided a new strategy to enhance the production and bioactivity of edible and medicinal fungal polysaccharides by using quorum sensing molecules.
34560149	10	64	with	polysaccharides	1715:1729	arg1	antioxidant					1741:1751	high antioxidant	1736:1751	high antioxidant	1736:1751	These results demonstrated that appropriate adjustment of culture conditions had potential application in the development of polysaccharides with high antioxidant and antitumor activity.
34560149	10	64	with	polysaccharides	1715:1729	arg1	activity					1767:1774	antitumor activity	1757:1774	antitumor activity	1757:1774	These results demonstrated that appropriate adjustment of culture conditions had potential application in the development of polysaccharides with high antioxidant and antitumor activity.
34560149	4	65	theme	antioxidant	703:713	arg1	activities					715:724	antioxidant activities	703:724	antioxidant activities	703:724	The physicochemical properties, antioxidant activities and antitumor activities were studied.
34560149	7	66	theme	scavenging	1157:1166	arg1	activities					1168:1177	strong scavenging activities	1150:1177	strong scavenging activities	1150:1177	Antioxidant experiments showed that they had strong scavenging activities on ABTS+, hydroxyl radical, O2- and DPPH radical.
34560149	3	67	theme	farnesol	648:655	arg1	conditions					622:631	the conditions	618:631	the conditions of control and farnesol	618:655	Four polysaccharides including EPS-C-0M, EPS-C-0.2M, EPS-F-0M and EPS-F-0.2M were extracted and purified under the conditions of control and farnesol respectively.
34560149	8	68	theme	human	1301:1305	arg1	cells					1330:1334	human cervical cancer (HeLa) cells	1301:1334	human cervical cancer (HeLa) cells	1301:1334	Antitumor experiments showed that they had strong inhibitory effects on human cervical cancer (HeLa) cells and human liver cancer cells (HepG2) cells.
34560149	5	69	theme	kDa	1014:1016	arg1	weights					953:959	the average molecular weights	931:959	the average molecular weights of 1.12 × 103, 1.89 × 103, 1.41 × 103 and 2.02 × 103 kDa	931:1016	Their chemical composition differed in sugar, protein and uronic acid contents, and they were composed of six constituent monosaccharides with different ratios, with the average molecular weights of 1.12 × 103, 1.89 × 103, 1.41 × 103 and 2.02 × 103 kDa, respectively.
34560149	10	70	theme	conditions	1656:1665	arg1	adjustment					1634:1643	appropriate adjustment	1622:1643	appropriate adjustment of culture conditions	1622:1665	These results demonstrated that appropriate adjustment of culture conditions had potential application in the development of polysaccharides with high antioxidant and antitumor activity.
34560149	10	71	from	application	1681:1691	arg1	development					1700:1710	the development	1696:1710	the development of polysaccharides with high antioxidant and antitumor activity	1696:1774	These results demonstrated that appropriate adjustment of culture conditions had potential application in the development of polysaccharides with high antioxidant and antitumor activity.
34560149	10	72	theme	high	1736:1739	arg1	antioxidant					1741:1751	high antioxidant	1736:1751	high antioxidant	1736:1751	These results demonstrated that appropriate adjustment of culture conditions had potential application in the development of polysaccharides with high antioxidant and antitumor activity.
34560149	11	73	theme	new	1791:1793	arg1	strategy					1795:1802	a new strategy	1789:1802	a new strategy to enhance the production and bioactivity of edible and medicinal fungal polysaccharides by using quorum sensing molecules	1789:1925	It provided a new strategy to enhance the production and bioactivity of edible and medicinal fungal polysaccharides by using quorum sensing molecules.
34560149	3	74	theme	control	636:642	arg1	conditions					622:631	the conditions	618:631	the conditions of control and farnesol	618:655	Four polysaccharides including EPS-C-0M, EPS-C-0.2M, EPS-F-0M and EPS-F-0.2M were extracted and purified under the conditions of control and farnesol respectively.
34560149	9	75	theme	EPS	1547:1549	arg1	activity					1535:1542	the biological activity	1520:1542	the biological activity of EPS	1520:1549	Among the four polysaccharides, EPS-F-0.2M showed the highest antioxidant and antitumor activities, indicating that farnesol could regulate the biological activity of EPS by affecting structure and properties.
34560149	8	76	theme	cancer	1316:1321	arg1	cells					1330:1334	human cervical cancer (HeLa) cells	1301:1334	human cervical cancer (HeLa) cells	1301:1334	Antitumor experiments showed that they had strong inhibitory effects on human cervical cancer (HeLa) cells and human liver cancer cells (HepG2) cells.
34560149	11	77	dep	production	1819:1828	arg1	the					1815:1817	the	1815:1817	the	1815:1817	It provided a new strategy to enhance the production and bioactivity of edible and medicinal fungal polysaccharides by using quorum sensing molecules.
34560149	8	78	theme	inhibitory	1279:1288	arg1	effects					1290:1296	strong inhibitory effects	1272:1296	strong inhibitory effects	1272:1296	Antitumor experiments showed that they had strong inhibitory effects on human cervical cancer (HeLa) cells and human liver cancer cells (HepG2) cells.
34560149	5	79	theme	constituent	875:885	arg1	monosaccharides					887:901	six constituent monosaccharides	871:901	six constituent monosaccharides with different ratios	871:923	Their chemical composition differed in sugar, protein and uronic acid contents, and they were composed of six constituent monosaccharides with different ratios, with the average molecular weights of 1.12 × 103, 1.89 × 103, 1.41 × 103 and 2.02 × 103 kDa, respectively.
34560149	6	80	theme	different	1075:1083	arg1	morphology					1093:1102	different surface morphology	1075:1102	different surface morphology	1075:1102	They presented similar FT-IR spectra, but different surface morphology.
34560149	7	81	theme	strong	1150:1155	arg1	activities					1168:1177	strong scavenging activities	1150:1177	strong scavenging activities	1150:1177	Antioxidant experiments showed that they had strong scavenging activities on ABTS+, hydroxyl radical, O2- and DPPH radical.
34560149	5	82	theme	acid	830:833	arg1	contents					835:842	sugar, protein and uronic acid contents	804:842	sugar, protein and uronic acid contents	804:842	Their chemical composition differed in sugar, protein and uronic acid contents, and they were composed of six constituent monosaccharides with different ratios, with the average molecular weights of 1.12 × 103, 1.89 × 103, 1.41 × 103 and 2.02 × 103 kDa, respectively.
34560149	7	83	theme	hydroxyl	1189:1196	arg1	ABTS+					1182:1186	ABTS+	1182:1186	ABTS+	1182:1186	Antioxidant experiments showed that they had strong scavenging activities on ABTS+, hydroxyl radical, O2- and DPPH radical.
34560149	7	83	theme	hydroxyl	1189:1196	arg1	radical					1198:1204	hydroxyl radical	1189:1204	hydroxyl radical	1189:1204	Antioxidant experiments showed that they had strong scavenging activities on ABTS+, hydroxyl radical, O2- and DPPH radical.
34560149	0	84	from	Grifola	111:117	arg1	activity					65:72	activity	65:72	activity	65:72	The effects of quorum sensing molecule farnesol on the yield and activity of extracellular polysaccharide from Grifola frondosa in liquid fermentation.
34560149	0	84	from	Grifola	111:117	arg1	polysaccharide					91:104	extracellular polysaccharide	77:104	extracellular polysaccharide from Grifola frondosa	77:126	The effects of quorum sensing molecule farnesol on the yield and activity of extracellular polysaccharide from Grifola frondosa in liquid fermentation.
34560149	0	84	from	Grifola	111:117	arg1	yield					55:59	yield	55:59	yield	55:59	The effects of quorum sensing molecule farnesol on the yield and activity of extracellular polysaccharide from Grifola frondosa in liquid fermentation.
35014459	3	0	theme	cells	488:492	arg1	action					478:483	the action	474:483	the action of cells (osteoblasts and osteoclasts)	474:522	Bone is dynamic tissue constantly reshaped by the action of cells (osteoblasts and osteoclasts).
35014459	7	1	theme	industrial	1276:1285	arg1	production					1292:1301	industrial meat production	1276:1301	industrial meat production	1276:1301	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	4	2	theme	bone	566:569	arg1	injuries					571:578	heal bone injuries	561:578	heal bone injuries	561:578	This activity is normally enough to heal bone injuries; however, in several conditions, when bone is subjected to fatal damages, self-renewal is difficult, if not even impossible, and a medical treatment is required.
35014459	0	3	theme	Tissue	169:174	arg1	Applications					188:199	Bone Tissue Engineering Applications	164:199	Bone Tissue Engineering Applications	164:199	Comparative Study on the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications.
35014459	11	4	theme	DBP	1949:1951	arg1	melanin					1905:1911	melanin	1905:1911	melanin	1905:1911	These results have been related with the higher amount of melanin, studied by fluorescence, of the YO DBP compared to Cornish cross and Pekin duck.
35014459	11	4	theme	DBP	1949:1951	arg1	amount					1895:1900	the higher amount	1884:1900	the higher amount	1884:1900	These results have been related with the higher amount of melanin, studied by fluorescence, of the YO DBP compared to Cornish cross and Pekin duck.
35014459	11	4	theme	DBP	1949:1951	arg1	DBP					1949:1951	the YO DBP	1942:1951	the YO DBP	1942:1951	These results have been related with the higher amount of melanin, studied by fluorescence, of the YO DBP compared to Cornish cross and Pekin duck.
35014459	7	5	theme	traditional	1179:1189	arg1	chicken					1206:1212	a traditional and rare black chicken	1177:1212	a traditional and rare black chicken from Korea	1177:1223	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	5	6	theme	common	793:798	arg1	procedures					809:818	the common surgical procedures	789:818	the common surgical procedures	789:818	The transplantation of a biomaterial is one of the common surgical procedures to overcome critical injuries.
35014459	9	7	theme	bone-specific	1507:1519	arg1	expression					1526:1535	bone-specific gene expression	1507:1535	bone-specific gene expression	1507:1535	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	5	8	theme	procedures	809:818	arg1	one					782:784	one	782:784	one	782:784	The transplantation of a biomaterial is one of the common surgical procedures to overcome critical injuries.
35014459	5	8	theme	procedures	809:818	arg1	transplantation					746:760	The transplantation	742:760	The transplantation of a biomaterial	742:777	The transplantation of a biomaterial is one of the common surgical procedures to overcome critical injuries.
35014459	5	8	theme	procedures	809:818	arg1	procedures					809:818	the common surgical procedures	789:818	the common surgical procedures	789:818	The transplantation of a biomaterial is one of the common surgical procedures to overcome critical injuries.
35014459	9	9	theme	expression	1526:1535	arg1	suitability					1492:1502	the cellular suitability	1479:1502	the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation	1479:1614	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	4	10	theme	several	593:599	arg1	conditions					601:610	several conditions	593:610	several conditions	593:610	This activity is normally enough to heal bone injuries; however, in several conditions, when bone is subjected to fatal damages, self-renewal is difficult, if not even impossible, and a medical treatment is required.
35014459	0	11	theme	Bone	107:110	arg1	Regeneration					112:123	the Bone Regeneration	103:123	the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications	103:199	Comparative Study on the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications.
35014459	9	12	link	marrow-derived	1422:1435	arg1	cells					1454:1458	rat bone marrow-derived mesenchymal stem cells	1413:1458	rat bone marrow-derived mesenchymal stem cells (rBMSCs)	1413:1467	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	9	12	link	marrow-derived	1422:1435	arg1	rBMSCs					1461:1466	rBMSCs	1461:1466	rBMSCs	1461:1466	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	9	13	theme	seeded	1541:1546	arg1	scaffolds					1555:1563	seeded GG-DBP scaffolds	1541:1563	seeded GG-DBP scaffolds	1541:1563	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	7	14	theme	Cornish	1234:1240	arg1	breeds					1265:1270	the most common breeds	1249:1270	the most common breeds for industrial meat production	1249:1301	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	7	14	theme	Cornish	1234:1240	arg1	cross					1242:1246	the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross	1159:1246	the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross	1159:1246	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	10	15	theme	other	1824:1828	arg1	sources					1838:1844	the other studied sources	1820:1844	the other studied sources	1820:1844	The scaffold containing a DBP derived from the Yeonsan Ogye (YO) bone showed higher levels of cell proliferation and osteogenic differentiation in comparison with the scaffold with the DBP obtained from the other studied sources.
35014459	0	16	theme	Composite	130:138	arg1	Scaffold					151:158	a Composite Gellan Gum Scaffold	128:158	a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications	128:199	Comparative Study on the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications.
35014459	4	17	dep	even	688:691	arg1	if					681:682	if	681:682	if	681:682	This activity is normally enough to heal bone injuries; however, in several conditions, when bone is subjected to fatal damages, self-renewal is difficult, if not even impossible, and a medical treatment is required.
35014459	9	18	theme	differentiation	1566:1580	arg1	capacity					1582:1589	differentiation capacity	1566:1589	differentiation capacity	1566:1589	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	12	19	theme	tissue	2079:2084	arg1	regeneration					2086:2097	bone tissue regeneration	2074:2097	bone tissue regeneration	2074:2097	Overall, this study clearly shows the use of YO DBP as a promising material in bone tissue regeneration.
35014459	0	20	theme	Gum	147:149	arg1	Scaffold					151:158	a Composite Gellan Gum Scaffold	128:158	a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications	128:199	Comparative Study on the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications.
35014459	10	21	theme	Ogye	1672:1675	arg1	bone					1682:1685	the Yeonsan Ogye (YO) bone	1660:1685	the Yeonsan Ogye (YO) bone	1660:1685	The scaffold containing a DBP derived from the Yeonsan Ogye (YO) bone showed higher levels of cell proliferation and osteogenic differentiation in comparison with the scaffold with the DBP obtained from the other studied sources.
35014459	7	22	theme	chicken	1206:1212	arg1	breeds					1265:1270	the most common breeds	1249:1270	the most common breeds for industrial meat production	1249:1301	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	7	22	theme	chicken	1206:1212	arg1	cross					1242:1246	the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross	1159:1246	the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross	1159:1246	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	4	23	theme	fatal	639:643	arg1	damages					645:651	fatal damages	639:651	fatal damages	639:651	This activity is normally enough to heal bone injuries; however, in several conditions, when bone is subjected to fatal damages, self-renewal is difficult, if not even impossible, and a medical treatment is required.
35014459	0	24	theme	Harvesting	49:58	arg1	Sources					60:66	the Different Harvesting Sources	35:66	the Different Harvesting Sources of Demineralized Bone Particles	35:98	Comparative Study on the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications.
35014459	11	25	theme	Cornish	1965:1971	arg1	cross					1973:1977	Cornish cross	1965:1977	Cornish cross	1965:1977	These results have been related with the higher amount of melanin, studied by fluorescence, of the YO DBP compared to Cornish cross and Pekin duck.
35014459	6	26	theme	demineralized	925:937	arg1	bone					939:942	demineralized bone powder (DBP)	925:955	demineralized bone powder (DBP)	925:955	In this study, we exploited the effect of the use of different sources of demineralized bone powder (DBP) in combination with gellan gum (GG) to form a GG-DBP hydrogel scaffold with the purpose of regenerating the bone tissue.
35014459	9	27	theme	marked	1596:1601	arg1	upregulation					1603:1614	marked upregulation	1596:1614	marked upregulation	1596:1614	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	8	28	theme	composite	1328:1336	arg1	scaffold					1338:1345	The composite scaffold	1324:1345	The composite scaffold	1324:1345	The composite scaffold has been tested both in vitro and in vivo.
35014459	9	29	dep	In	1390:1391	arg1	vitro					1393:1397	vitro	1393:1397	vitro	1393:1397	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	6	30	theme	sources	914:920	arg1	use					897:899	the use	893:899	the use of different sources of demineralized bone powder (DBP)	893:955	In this study, we exploited the effect of the use of different sources of demineralized bone powder (DBP) in combination with gellan gum (GG) to form a GG-DBP hydrogel scaffold with the purpose of regenerating the bone tissue.
35014459	0	31	theme	Bone	85:88	arg1	Particles					90:98	Demineralized Bone Particles	71:98	Demineralized Bone Particles	71:98	Comparative Study on the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications.
35014459	7	32	theme	meat	1287:1290	arg1	production					1292:1301	industrial meat production	1276:1301	industrial meat production	1276:1301	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	7	33	dep	domesticus	1147:1156	arg1	breeds					1265:1270	the most common breeds	1249:1270	the most common breeds for industrial meat production	1249:1301	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	7	33	dep	domesticus	1147:1156	arg1	cross					1242:1246	the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross	1159:1246	the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross	1159:1246	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	12	34	theme	YO	2040:2041	arg1	DBP					2043:2045	YO DBP	2040:2045	YO DBP	2040:2045	Overall, this study clearly shows the use of YO DBP as a promising material in bone tissue regeneration.
35014459	4	35	theme	medical	711:717	arg1	treatment					719:727	a medical treatment	709:727	a medical treatment	709:727	This activity is normally enough to heal bone injuries; however, in several conditions, when bone is subjected to fatal damages, self-renewal is difficult, if not even impossible, and a medical treatment is required.
35014459	2	36	theme	rest	381:384	arg1	conditions					386:395	the rest conditions	377:395	the rest conditions	377:395	The material for bone regeneration has to provide the mechanical stability by maintaining the mechanical loads both in the rest conditions and during the body movements.
35014459	7	37	from	Korea	1219:1223	arg1	chicken					1206:1212	a traditional and rare black chicken	1177:1212	a traditional and rare black chicken from Korea	1177:1223	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	7	38	theme	black	1200:1204	arg1	chicken					1206:1212	a traditional and rare black chicken	1177:1212	a traditional and rare black chicken from Korea	1177:1223	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	0	39	from	Effect	25:30	arg1	Regeneration					112:123	the Bone Regeneration	103:123	the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications	103:199	Comparative Study on the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications.
35014459	0	40	theme	Comparative	0:10	arg1	Study					12:16	Comparative Study	0:16	Comparative Study on the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications.	0:200	Comparative Study on the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications.
35014459	3	41	dep	cells	488:492	arg1	osteoclasts					511:521	osteoclasts	511:521	osteoclasts	511:521	Bone is dynamic tissue constantly reshaped by the action of cells (osteoblasts and osteoclasts).
35014459	3	41	dep	cells	488:492	arg1	osteoblasts					495:505	osteoblasts	495:505	osteoblasts	495:505	Bone is dynamic tissue constantly reshaped by the action of cells (osteoblasts and osteoclasts).
35014459	9	42	theme	bone	1417:1420	arg1	cells					1454:1458	rat bone marrow-derived mesenchymal stem cells	1413:1458	rat bone marrow-derived mesenchymal stem cells (rBMSCs)	1413:1467	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	9	42	theme	bone	1417:1420	arg1	rBMSCs					1461:1466	rBMSCs	1461:1466	rBMSCs	1461:1466	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	7	43	theme	Ogye	1171:1174	arg1	breeds					1265:1270	the most common breeds	1249:1270	the most common breeds for industrial meat production	1249:1301	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	7	43	theme	Ogye	1171:1174	arg1	cross					1242:1246	the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross	1159:1246	the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross	1159:1246	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	11	44	theme	Pekin	1983:1987	arg1	duck					1989:1992	Pekin duck	1983:1992	Pekin duck	1983:1992	These results have been related with the higher amount of melanin, studied by fluorescence, of the YO DBP compared to Cornish cross and Pekin duck.
35014459	10	45	theme	YO	1678:1679	arg1	bone					1682:1685	the Yeonsan Ogye (YO) bone	1660:1685	the Yeonsan Ogye (YO) bone	1660:1685	The scaffold containing a DBP derived from the Yeonsan Ogye (YO) bone showed higher levels of cell proliferation and osteogenic differentiation in comparison with the scaffold with the DBP obtained from the other studied sources.
35014459	11	46	theme	YO	1946:1947	arg1	DBP					1949:1951	the YO DBP	1942:1951	the YO DBP	1942:1951	These results have been related with the higher amount of melanin, studied by fluorescence, of the YO DBP compared to Cornish cross and Pekin duck.
35014459	9	47	theme	mesenchymal	1437:1447	arg1	cells					1454:1458	rat bone marrow-derived mesenchymal stem cells	1413:1458	rat bone marrow-derived mesenchymal stem cells (rBMSCs)	1413:1467	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	9	47	theme	mesenchymal	1437:1447	arg1	rBMSCs					1461:1466	rBMSCs	1461:1466	rBMSCs	1461:1466	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	6	48	theme	hydrogel	1010:1017	arg1	scaffold					1019:1026	a GG-DBP hydrogel scaffold	1001:1026	a GG-DBP hydrogel scaffold	1001:1026	In this study, we exploited the effect of the use of different sources of demineralized bone powder (DBP) in combination with gellan gum (GG) to form a GG-DBP hydrogel scaffold with the purpose of regenerating the bone tissue.
35014459	2	49	theme	mechanical	312:321	arg1	stability					323:331	the mechanical stability	308:331	the mechanical stability	308:331	The material for bone regeneration has to provide the mechanical stability by maintaining the mechanical loads both in the rest conditions and during the body movements.
35014459	4	50	from	difficult	670:678	arg1	conditions					601:610	several conditions	593:610	several conditions	593:610	This activity is normally enough to heal bone injuries; however, in several conditions, when bone is subjected to fatal damages, self-renewal is difficult, if not even impossible, and a medical treatment is required.
35014459	6	51	with	combination	960:970	arg1	GG					989:990	GG	989:990	GG	989:990	In this study, we exploited the effect of the use of different sources of demineralized bone powder (DBP) in combination with gellan gum (GG) to form a GG-DBP hydrogel scaffold with the purpose of regenerating the bone tissue.
35014459	6	51	with	combination	960:970	arg1	gum					984:986	gellan gum	977:986	gellan gum (GG)	977:991	In this study, we exploited the effect of the use of different sources of demineralized bone powder (DBP) in combination with gellan gum (GG) to form a GG-DBP hydrogel scaffold with the purpose of regenerating the bone tissue.
35014459	10	52	theme	higher	1694:1699	arg1	levels					1701:1706	higher levels	1694:1706	higher levels of cell proliferation and osteogenic differentiation	1694:1759	The scaffold containing a DBP derived from the Yeonsan Ogye (YO) bone showed higher levels of cell proliferation and osteogenic differentiation in comparison with the scaffold with the DBP obtained from the other studied sources.
35014459	7	53	theme	Gallus	1133:1138	arg1	gallus					1140:1145	Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production)	1133:1302	Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production)	1133:1302	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	10	54	theme	osteogenic	1734:1743	arg1	differentiation					1745:1759	osteogenic differentiation	1734:1759	osteogenic differentiation	1734:1759	The scaffold containing a DBP derived from the Yeonsan Ogye (YO) bone showed higher levels of cell proliferation and osteogenic differentiation in comparison with the scaffold with the DBP obtained from the other studied sources.
35014459	0	55	theme	Bone	164:167	arg1	Applications					188:199	Bone Tissue Engineering Applications	164:199	Bone Tissue Engineering Applications	164:199	Comparative Study on the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications.
35014459	12	56	theme	promising	2052:2060	arg1	material					2062:2069	a promising material	2050:2069	a promising material in bone tissue regeneration	2050:2097	Overall, this study clearly shows the use of YO DBP as a promising material in bone tissue regeneration.
35014459	7	57	theme	Pekin	1312:1316	arg1	duck					1318:1321	the Pekin duck	1308:1321	the Pekin duck	1308:1321	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	7	58	theme	typologies	1119:1128	arg1	duck					1318:1321	the Pekin duck	1308:1321	the Pekin duck	1308:1321	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	7	58	theme	typologies	1119:1128	arg1	femurs					1105:1110	the femurs	1101:1110	the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production)	1101:1302	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	10	59	theme	proliferation	1716:1728	arg1	levels					1701:1706	higher levels	1694:1706	higher levels of cell proliferation and osteogenic differentiation	1694:1759	The scaffold containing a DBP derived from the Yeonsan Ogye (YO) bone showed higher levels of cell proliferation and osteogenic differentiation in comparison with the scaffold with the DBP obtained from the other studied sources.
35014459	0	60	theme	Engineering	176:186	arg1	Applications					188:199	Bone Tissue Engineering Applications	164:199	Bone Tissue Engineering Applications	164:199	Comparative Study on the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications.
35014459	2	61	theme	bone	275:278	arg1	regeneration					280:291	bone regeneration	275:291	bone regeneration	275:291	The material for bone regeneration has to provide the mechanical stability by maintaining the mechanical loads both in the rest conditions and during the body movements.
35014459	9	62	theme	cellular	1483:1490	arg1	suitability					1492:1502	the cellular suitability	1479:1502	the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation	1479:1614	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	6	63	theme	bone	1065:1068	arg1	tissue					1070:1075	the bone tissue	1061:1075	the bone tissue	1061:1075	In this study, we exploited the effect of the use of different sources of demineralized bone powder (DBP) in combination with gellan gum (GG) to form a GG-DBP hydrogel scaffold with the purpose of regenerating the bone tissue.
35014459	4	64	theme	heal	561:564	arg1	injuries					571:578	heal bone injuries	561:578	heal bone injuries	561:578	This activity is normally enough to heal bone injuries; however, in several conditions, when bone is subjected to fatal damages, self-renewal is difficult, if not even impossible, and a medical treatment is required.
35014459	5	65	theme	surgical	800:807	arg1	procedures					809:818	the common surgical procedures	789:818	the common surgical procedures	789:818	The transplantation of a biomaterial is one of the common surgical procedures to overcome critical injuries.
35014459	12	66	theme	bone	2074:2077	arg1	regeneration					2086:2097	bone tissue regeneration	2074:2097	bone tissue regeneration	2074:2097	Overall, this study clearly shows the use of YO DBP as a promising material in bone tissue regeneration.
35014459	6	67	theme	use	897:899	arg1	effect					883:888	the effect	879:888	the effect of the use of different sources of demineralized bone powder (DBP) in combination with gellan gum (GG)	879:991	In this study, we exploited the effect of the use of different sources of demineralized bone powder (DBP) in combination with gellan gum (GG) to form a GG-DBP hydrogel scaffold with the purpose of regenerating the bone tissue.
35014459	9	68	theme	gene	1521:1524	arg1	expression					1526:1535	bone-specific gene expression	1507:1535	bone-specific gene expression	1507:1535	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	7	69	theme	common	1258:1263	arg1	breeds					1265:1270	the most common breeds	1249:1270	the most common breeds for industrial meat production	1249:1301	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	7	69	theme	common	1258:1263	arg1	cross					1242:1246	the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross	1159:1246	the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross	1159:1246	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	4	70	from	conditions	601:610	arg1	difficult					670:678	difficult	670:678	difficult	670:678	This activity is normally enough to heal bone injuries; however, in several conditions, when bone is subjected to fatal damages, self-renewal is difficult, if not even impossible, and a medical treatment is required.
35014459	5	71	theme	biomaterial	767:777	arg1	one					782:784	one	782:784	one	782:784	The transplantation of a biomaterial is one of the common surgical procedures to overcome critical injuries.
35014459	5	71	theme	biomaterial	767:777	arg1	transplantation					746:760	The transplantation	742:760	The transplantation of a biomaterial	742:777	The transplantation of a biomaterial is one of the common surgical procedures to overcome critical injuries.
35014459	5	71	theme	biomaterial	767:777	arg1	procedures					809:818	the common surgical procedures	789:818	the common surgical procedures	789:818	The transplantation of a biomaterial is one of the common surgical procedures to overcome critical injuries.
35014459	10	72	attach	derived	1647:1653	arg1	bone					1682:1685	the Yeonsan Ogye (YO) bone	1660:1685	the Yeonsan Ogye (YO) bone	1660:1685	The scaffold containing a DBP derived from the Yeonsan Ogye (YO) bone showed higher levels of cell proliferation and osteogenic differentiation in comparison with the scaffold with the DBP obtained from the other studied sources.
35014459	10	72	attach	derived	1647:1653	arg2	DBP					1643:1645	a DBP	1641:1645	a DBP derived from the Yeonsan Ogye (YO) bone	1641:1685	The scaffold containing a DBP derived from the Yeonsan Ogye (YO) bone showed higher levels of cell proliferation and osteogenic differentiation in comparison with the scaffold with the DBP obtained from the other studied sources.
35014459	9	73	theme	GG-DBP	1548:1553	arg1	scaffolds					1555:1563	seeded GG-DBP scaffolds	1541:1563	seeded GG-DBP scaffolds	1541:1563	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	12	74	from	material	2062:2069	arg1	regeneration					2086:2097	bone tissue regeneration	2074:2097	bone tissue regeneration	2074:2097	Overall, this study clearly shows the use of YO DBP as a promising material in bone tissue regeneration.
35014459	0	75	theme	Gellan	140:145	arg1	Scaffold					151:158	a Composite Gellan Gum Scaffold	128:158	a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications	128:199	Comparative Study on the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications.
35014459	11	76	theme	higher	1888:1893	arg1	melanin					1905:1911	melanin	1905:1911	melanin	1905:1911	These results have been related with the higher amount of melanin, studied by fluorescence, of the YO DBP compared to Cornish cross and Pekin duck.
35014459	11	76	theme	higher	1888:1893	arg1	amount					1895:1900	the higher amount	1884:1900	the higher amount	1884:1900	These results have been related with the higher amount of melanin, studied by fluorescence, of the YO DBP compared to Cornish cross and Pekin duck.
35014459	11	76	theme	higher	1888:1893	arg1	DBP					1949:1951	the YO DBP	1942:1951	the YO DBP	1942:1951	These results have been related with the higher amount of melanin, studied by fluorescence, of the YO DBP compared to Cornish cross and Pekin duck.
35014459	0	77	theme	Scaffold	151:158	arg1	Regeneration					112:123	the Bone Regeneration	103:123	the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications	103:199	Comparative Study on the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications.
35014459	11	78	theme	melanin	1905:1911	arg1	melanin					1905:1911	melanin	1905:1911	melanin	1905:1911	These results have been related with the higher amount of melanin, studied by fluorescence, of the YO DBP compared to Cornish cross and Pekin duck.
35014459	11	78	theme	melanin	1905:1911	arg1	amount					1895:1900	the higher amount	1884:1900	the higher amount	1884:1900	These results have been related with the higher amount of melanin, studied by fluorescence, of the YO DBP compared to Cornish cross and Pekin duck.
35014459	11	78	theme	melanin	1905:1911	arg1	DBP					1949:1951	the YO DBP	1942:1951	the YO DBP	1942:1951	These results have been related with the higher amount of melanin, studied by fluorescence, of the YO DBP compared to Cornish cross and Pekin duck.
35014459	7	79	theme	gallus	1140:1145	arg1	typologies					1119:1128	two typologies	1115:1128	two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production)	1115:1302	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	0	80	theme	Sources	60:66	arg1	Effect					25:30	the Effect	21:30	the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications	21:199	Comparative Study on the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications.
35014459	10	81	theme	Yeonsan	1664:1670	arg1	bone					1682:1685	the Yeonsan Ogye (YO) bone	1660:1685	the Yeonsan Ogye (YO) bone	1660:1685	The scaffold containing a DBP derived from the Yeonsan Ogye (YO) bone showed higher levels of cell proliferation and osteogenic differentiation in comparison with the scaffold with the DBP obtained from the other studied sources.
35014459	6	82	theme	bone	939:942	arg1	sources					914:920	different sources	904:920	different sources of demineralized bone powder (DBP)	904:955	In this study, we exploited the effect of the use of different sources of demineralized bone powder (DBP) in combination with gellan gum (GG) to form a GG-DBP hydrogel scaffold with the purpose of regenerating the bone tissue.
35014459	10	83	theme	studied	1830:1836	arg1	sources					1838:1844	the other studied sources	1820:1844	the other studied sources	1820:1844	The scaffold containing a DBP derived from the Yeonsan Ogye (YO) bone showed higher levels of cell proliferation and osteogenic differentiation in comparison with the scaffold with the DBP obtained from the other studied sources.
35014459	0	84	theme	Demineralized	71:83	arg1	Particles					90:98	Demineralized Bone Particles	71:98	Demineralized Bone Particles	71:98	Comparative Study on the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications.
35014459	5	85	theme	critical	832:839	arg1	injuries					841:848	critical injuries	832:848	critical injuries	832:848	The transplantation of a biomaterial is one of the common surgical procedures to overcome critical injuries.
35014459	12	86	theme	DBP	2043:2045	arg1	use					2033:2035	the use	2029:2035	the use of YO DBP as a promising material in bone tissue regeneration	2029:2097	Overall, this study clearly shows the use of YO DBP as a promising material in bone tissue regeneration.
35014459	0	87	from	Study	12:16	arg1	Effect					25:30	the Effect	21:30	the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications	21:199	Comparative Study on the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications.
35014459	0	88	theme	Particles	90:98	arg1	Sources					60:66	the Different Harvesting Sources	35:66	the Different Harvesting Sources of Demineralized Bone Particles	35:98	Comparative Study on the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications.
35014459	7	89	dep	gallus	1140:1145	arg1	domesticus					1147:1156	domesticus	1147:1156	Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production)	1133:1302	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	6	90	theme	different	904:912	arg1	sources					914:920	different sources	904:920	different sources of demineralized bone powder (DBP)	904:955	In this study, we exploited the effect of the use of different sources of demineralized bone powder (DBP) in combination with gellan gum (GG) to form a GG-DBP hydrogel scaffold with the purpose of regenerating the bone tissue.
35014459	6	91	dep	bone	939:942	arg1	DBP					952:954	DBP	952:954	DBP	952:954	In this study, we exploited the effect of the use of different sources of demineralized bone powder (DBP) in combination with gellan gum (GG) to form a GG-DBP hydrogel scaffold with the purpose of regenerating the bone tissue.
35014459	6	91	dep	bone	939:942	arg1	powder					944:949	powder	944:949	demineralized bone powder (DBP)	925:955	In this study, we exploited the effect of the use of different sources of demineralized bone powder (DBP) in combination with gellan gum (GG) to form a GG-DBP hydrogel scaffold with the purpose of regenerating the bone tissue.
35014459	7	92	theme	rare	1195:1198	arg1	chicken					1206:1212	a traditional and rare black chicken	1177:1212	a traditional and rare black chicken from Korea	1177:1223	DBP was extracted from the femurs of two typologies of Gallus gallus domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck.
35014459	10	93	contain	containing	1630:1639	arg1	scaffold					1621:1628	The scaffold	1617:1628	The scaffold containing a DBP derived from the Yeonsan Ogye (YO) bone	1617:1685	The scaffold containing a DBP derived from the Yeonsan Ogye (YO) bone showed higher levels of cell proliferation and osteogenic differentiation in comparison with the scaffold with the DBP obtained from the other studied sources.
35014459	10	93	contain	containing	1630:1639	arg2	DBP					1643:1645	a DBP	1641:1645	a DBP derived from the Yeonsan Ogye (YO) bone	1641:1685	The scaffold containing a DBP derived from the Yeonsan Ogye (YO) bone showed higher levels of cell proliferation and osteogenic differentiation in comparison with the scaffold with the DBP obtained from the other studied sources.
35014459	9	94	theme	In	1390:1391	arg1	studies					1399:1405	In vitro studies	1390:1405	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs)	1390:1467	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	6	95	theme	gellan	977:982	arg1	GG					989:990	GG	989:990	GG	989:990	In this study, we exploited the effect of the use of different sources of demineralized bone powder (DBP) in combination with gellan gum (GG) to form a GG-DBP hydrogel scaffold with the purpose of regenerating the bone tissue.
35014459	6	95	theme	gellan	977:982	arg1	gum					984:986	gellan gum	977:986	gellan gum (GG)	977:991	In this study, we exploited the effect of the use of different sources of demineralized bone powder (DBP) in combination with gellan gum (GG) to form a GG-DBP hydrogel scaffold with the purpose of regenerating the bone tissue.
35014459	10	96	with	comparison	1764:1773	arg1	scaffold					1784:1791	the scaffold	1780:1791	the scaffold	1780:1791	The scaffold containing a DBP derived from the Yeonsan Ogye (YO) bone showed higher levels of cell proliferation and osteogenic differentiation in comparison with the scaffold with the DBP obtained from the other studied sources.
35014459	10	96	with	comparison	1764:1773	arg1	DBP					1802:1804	the DBP	1798:1804	the DBP obtained from the other studied sources	1798:1844	The scaffold containing a DBP derived from the Yeonsan Ogye (YO) bone showed higher levels of cell proliferation and osteogenic differentiation in comparison with the scaffold with the DBP obtained from the other studied sources.
35014459	2	97	theme	body	412:415	arg1	movements					417:425	the body movements	408:425	the body movements	408:425	The material for bone regeneration has to provide the mechanical stability by maintaining the mechanical loads both in the rest conditions and during the body movements.
35014459	6	98	from	effect	883:888	arg1	combination					960:970	combination	960:970	combination with gellan gum (GG)	960:991	In this study, we exploited the effect of the use of different sources of demineralized bone powder (DBP) in combination with gellan gum (GG) to form a GG-DBP hydrogel scaffold with the purpose of regenerating the bone tissue.
35014459	9	99	theme	rat	1413:1415	arg1	cells					1454:1458	rat bone marrow-derived mesenchymal stem cells	1413:1458	rat bone marrow-derived mesenchymal stem cells (rBMSCs)	1413:1467	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	9	99	theme	rat	1413:1415	arg1	rBMSCs					1461:1466	rBMSCs	1461:1466	rBMSCs	1461:1466	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	3	100	theme	dynamic	436:442	arg1	tissue					444:449	dynamic tissue	436:449	dynamic tissue constantly reshaped by the action of cells (osteoblasts and osteoclasts)	436:522	Bone is dynamic tissue constantly reshaped by the action of cells (osteoblasts and osteoclasts).
35014459	0	101	theme	Different	39:47	arg1	Sources					60:66	the Different Harvesting Sources	35:66	the Different Harvesting Sources of Demineralized Bone Particles	35:98	Comparative Study on the Effect of the Different Harvesting Sources of Demineralized Bone Particles on the Bone Regeneration of a Composite Gellan Gum Scaffold for Bone Tissue Engineering Applications.
35014459	9	102	theme	marrow-derived	1422:1435	arg1	cells					1454:1458	rat bone marrow-derived mesenchymal stem cells	1413:1458	rat bone marrow-derived mesenchymal stem cells (rBMSCs)	1413:1467	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	9	102	theme	marrow-derived	1422:1435	arg1	rBMSCs					1461:1466	rBMSCs	1461:1466	rBMSCs	1461:1466	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	10	103	theme	cell	1711:1714	arg1	proliferation					1716:1728	cell proliferation	1711:1728	cell proliferation	1711:1728	The scaffold containing a DBP derived from the Yeonsan Ogye (YO) bone showed higher levels of cell proliferation and osteogenic differentiation in comparison with the scaffold with the DBP obtained from the other studied sources.
35014459	9	104	theme	stem	1449:1452	arg1	cells					1454:1458	rat bone marrow-derived mesenchymal stem cells	1413:1458	rat bone marrow-derived mesenchymal stem cells (rBMSCs)	1413:1467	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	9	104	theme	stem	1449:1452	arg1	rBMSCs					1461:1466	rBMSCs	1461:1466	rBMSCs	1461:1466	In vitro studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation.
35014459	6	105	theme	GG-DBP	1003:1008	arg1	scaffold					1019:1026	a GG-DBP hydrogel scaffold	1001:1026	a GG-DBP hydrogel scaffold	1001:1026	In this study, we exploited the effect of the use of different sources of demineralized bone powder (DBP) in combination with gellan gum (GG) to form a GG-DBP hydrogel scaffold with the purpose of regenerating the bone tissue.
35014459	1	106	theme	rigid	214:218	arg1	tissue					220:225	the rigid tissue	210:225	the rigid tissue that constitutes the skeleton	210:255	Bone is the rigid tissue that constitutes the skeleton.
35014459	1	106	theme	rigid	214:218	arg1	Bone					202:205	Bone	202:205	Bone	202:205	Bone is the rigid tissue that constitutes the skeleton.
35014459	10	107	theme	differentiation	1745:1759	arg1	levels					1701:1706	higher levels	1694:1706	higher levels of cell proliferation and osteogenic differentiation	1694:1759	The scaffold containing a DBP derived from the Yeonsan Ogye (YO) bone showed higher levels of cell proliferation and osteogenic differentiation in comparison with the scaffold with the DBP obtained from the other studied sources.
35014459	2	108	theme	mechanical	352:361	arg1	loads					363:367	the mechanical loads	348:367	the mechanical loads	348:367	The material for bone regeneration has to provide the mechanical stability by maintaining the mechanical loads both in the rest conditions and during the body movements.
34848094	0	0	theme	dextran-conjugated	88:105	arg1	protein					139:145	dextran-conjugated clam Meretrix meretrix linnaeus protein	88:145	dextran-conjugated clam Meretrix meretrix linnaeus protein isolate	88:153	Preparation, characterization, and gel characteristics of nanoemulsions stabilized with dextran-conjugated clam Meretrix meretrix linnaeus protein isolate.
34848094	2	1	theme	CPI-Dex	505:511	arg1	nanoemulsions					517:529	CPI-Dex Mix nanoemulsions	505:529	CPI-Dex Mix nanoemulsions	505:529	Compared with the NCPI (CPI nanoemulsions) and NCPI-Dex Mix (CPI-Dex Mix nanoemulsions), the NCPI-Dex Con (CPI-Dex Con nanoemulsions) has better stability and flavor.
34848094	2	1	theme	CPI-Dex	505:511	arg1	Mix					500:502	NCPI-Dex Mix	491:502	NCPI-Dex Mix (CPI-Dex Mix nanoemulsions)	491:530	Compared with the NCPI (CPI nanoemulsions) and NCPI-Dex Mix (CPI-Dex Mix nanoemulsions), the NCPI-Dex Con (CPI-Dex Con nanoemulsions) has better stability and flavor.
34848094	1	2	theme	protein	285:291	arg1	isolate					293:299	dextran-conjugated Meretrix meretrix clam protein isolate	243:299	dextran-conjugated Meretrix meretrix clam protein isolate	243:299	In this study, the stability and flavor characteristics of nanoemulsions prepared with dextran-conjugated Meretrix meretrix clam protein isolate were studied by characterizing particle size, polydispersity index, zeta potential, turbidity, microstructure, e-tongue, e-nose and HS-GC-IMS.
34848094	2	3	theme	CPI-Dex	551:557	arg1	Con					546:548	the NCPI-Dex Con	533:548	the NCPI-Dex Con (CPI-Dex Con nanoemulsions)	533:576	Compared with the NCPI (CPI nanoemulsions) and NCPI-Dex Mix (CPI-Dex Mix nanoemulsions), the NCPI-Dex Con (CPI-Dex Con nanoemulsions) has better stability and flavor.
34848094	2	3	theme	CPI-Dex	551:557	arg1	nanoemulsions					563:575	CPI-Dex Con nanoemulsions	551:575	CPI-Dex Con nanoemulsions	551:575	Compared with the NCPI (CPI nanoemulsions) and NCPI-Dex Mix (CPI-Dex Mix nanoemulsions), the NCPI-Dex Con (CPI-Dex Con nanoemulsions) has better stability and flavor.
34848094	0	4	theme	Meretrix	112:119	arg1	protein					139:145	dextran-conjugated clam Meretrix meretrix linnaeus protein	88:145	dextran-conjugated clam Meretrix meretrix linnaeus protein isolate	88:153	Preparation, characterization, and gel characteristics of nanoemulsions stabilized with dextran-conjugated clam Meretrix meretrix linnaeus protein isolate.
34848094	6	5	dep	Con	1037:1039	arg1	CPI-Dex					1029:1035	CPI-Dex	1029:1035	CPI-Dex	1029:1035	Therefore, CPI-Dex Con stabilized nanoemulsions could effectively improve the gel property and flavor of the clam sausages.
34848094	6	5	dep	Con	1037:1039	arg1	stabilized					1041:1050	stabilized	1041:1050	stabilized	1041:1050	Therefore, CPI-Dex Con stabilized nanoemulsions could effectively improve the gel property and flavor of the clam sausages.
34848094	5	6	theme	8	918:918	arg1	%					919:919	%	919:919	%	919:919	The clam sausages supplemented with the 8% NCPI-Dex Con had the highest sensory score, with the densest and the most uniform gel structure.
34848094	5	7	contain	had	934:936	arg1	sausages					887:894	The clam sausages	878:894	The clam sausages supplemented with the 8% NCPI-Dex Con	878:932	The clam sausages supplemented with the 8% NCPI-Dex Con had the highest sensory score, with the densest and the most uniform gel structure.
34848094	5	7	contain	had	934:936	arg2	score					958:962	the highest sensory score	938:962	the highest sensory score	938:962	The clam sausages supplemented with the 8% NCPI-Dex Con had the highest sensory score, with the densest and the most uniform gel structure.
34848094	3	8	dep	significantly	675:687	arg1	P > 0.05					690:697	P > 0.05	690:697	P > 0.05	690:697	The breaking strength and breaking strain of clam sausages were significantly (P > 0.05) affected by the addition of NCPI-Dex Con.
34848094	0	9	theme	clam	107:110	arg1	protein					139:145	dextran-conjugated clam Meretrix meretrix linnaeus protein	88:145	dextran-conjugated clam Meretrix meretrix linnaeus protein isolate	88:153	Preparation, characterization, and gel characteristics of nanoemulsions stabilized with dextran-conjugated clam Meretrix meretrix linnaeus protein isolate.
34848094	5	10	theme	%	919:919	arg1	Con					930:932	the 8% NCPI-Dex Con	914:932	the 8% NCPI-Dex Con	914:932	The clam sausages supplemented with the 8% NCPI-Dex Con had the highest sensory score, with the densest and the most uniform gel structure.
34848094	5	11	theme	clam	882:885	arg1	sausages					887:894	The clam sausages	878:894	The clam sausages supplemented with the 8% NCPI-Dex Con	878:932	The clam sausages supplemented with the 8% NCPI-Dex Con had the highest sensory score, with the densest and the most uniform gel structure.
34848094	0	12	theme	linnaeus	130:137	arg1	protein					139:145	dextran-conjugated clam Meretrix meretrix linnaeus protein	88:145	dextran-conjugated clam Meretrix meretrix linnaeus protein isolate	88:153	Preparation, characterization, and gel characteristics of nanoemulsions stabilized with dextran-conjugated clam Meretrix meretrix linnaeus protein isolate.
34848094	3	13	theme	NCPI-Dex	728:735	arg1	Con					737:739	NCPI-Dex Con	728:739	NCPI-Dex Con	728:739	The breaking strength and breaking strain of clam sausages were significantly (P > 0.05) affected by the addition of NCPI-Dex Con.
34848094	4	14	theme	%	765:765	arg1	Con					776:778	8% NCPI-Dex Con	764:778	8% NCPI-Dex Con	764:778	The gel strength with 8% NCPI-Dex Con was the highest (5122.08 g‧mm), a 51.07% increase compared with the control group (3390.58 g‧mm).
34848094	0	15	theme	meretrix	121:128	arg1	protein					139:145	dextran-conjugated clam Meretrix meretrix linnaeus protein	88:145	dextran-conjugated clam Meretrix meretrix linnaeus protein isolate	88:153	Preparation, characterization, and gel characteristics of nanoemulsions stabilized with dextran-conjugated clam Meretrix meretrix linnaeus protein isolate.
34848094	4	16	theme	8	764:764	arg1	%					765:765	%	765:765	%	765:765	The gel strength with 8% NCPI-Dex Con was the highest (5122.08 g‧mm), a 51.07% increase compared with the control group (3390.58 g‧mm).
34848094	4	17	theme	%	819:819	arg1	highest					788:794	highest	788:794	highest	788:794	The gel strength with 8% NCPI-Dex Con was the highest (5122.08 g‧mm), a 51.07% increase compared with the control group (3390.58 g‧mm).
34848094	4	17	theme	%	819:819	arg1	increase					821:828	a 51.07% increase	812:828	a 51.07% increase compared with the control group (3390.58 g‧mm)	812:875	The gel strength with 8% NCPI-Dex Con was the highest (5122.08 g‧mm), a 51.07% increase compared with the control group (3390.58 g‧mm).
34848094	1	18	theme	flavor	189:194	arg1	characteristics					196:210	flavor characteristics	189:210	flavor characteristics	189:210	In this study, the stability and flavor characteristics of nanoemulsions prepared with dextran-conjugated Meretrix meretrix clam protein isolate were studied by characterizing particle size, polydispersity index, zeta potential, turbidity, microstructure, e-tongue, e-nose and HS-GC-IMS.
34848094	2	19	theme	CPI	468:470	arg1	NCPI					462:465	the NCPI	458:465	the NCPI (CPI nanoemulsions)	458:485	Compared with the NCPI (CPI nanoemulsions) and NCPI-Dex Mix (CPI-Dex Mix nanoemulsions), the NCPI-Dex Con (CPI-Dex Con nanoemulsions) has better stability and flavor.
34848094	2	19	theme	CPI	468:470	arg1	nanoemulsions					472:484	CPI nanoemulsions	468:484	CPI nanoemulsions	468:484	Compared with the NCPI (CPI nanoemulsions) and NCPI-Dex Mix (CPI-Dex Mix nanoemulsions), the NCPI-Dex Con (CPI-Dex Con nanoemulsions) has better stability and flavor.
34848094	4	20	theme	51.07	814:818	arg1	%					819:819	%	819:819	%	819:819	The gel strength with 8% NCPI-Dex Con was the highest (5122.08 g‧mm), a 51.07% increase compared with the control group (3390.58 g‧mm).
34848094	6	21	theme	gel	1096:1098	arg1	property					1100:1107	the gel property	1092:1107	the gel property	1092:1107	Therefore, CPI-Dex Con stabilized nanoemulsions could effectively improve the gel property and flavor of the clam sausages.
34848094	5	22	theme	gel	1003:1005	arg1	structure					1007:1015	the most uniform gel structure	986:1015	the most uniform gel structure	986:1015	The clam sausages supplemented with the 8% NCPI-Dex Con had the highest sensory score, with the densest and the most uniform gel structure.
34848094	6	23	theme	sausages	1132:1139	arg1	flavor					1113:1118	flavor	1113:1118	flavor	1113:1118	Therefore, CPI-Dex Con stabilized nanoemulsions could effectively improve the gel property and flavor of the clam sausages.
34848094	6	23	theme	sausages	1132:1139	arg1	property					1100:1107	the gel property	1092:1107	the gel property	1092:1107	Therefore, CPI-Dex Con stabilized nanoemulsions could effectively improve the gel property and flavor of the clam sausages.
34848094	2	24	theme	Con	559:561	arg1	Con					546:548	the NCPI-Dex Con	533:548	the NCPI-Dex Con (CPI-Dex Con nanoemulsions)	533:576	Compared with the NCPI (CPI nanoemulsions) and NCPI-Dex Mix (CPI-Dex Mix nanoemulsions), the NCPI-Dex Con (CPI-Dex Con nanoemulsions) has better stability and flavor.
34848094	2	24	theme	Con	559:561	arg1	nanoemulsions					563:575	CPI-Dex Con nanoemulsions	551:575	CPI-Dex Con nanoemulsions	551:575	Compared with the NCPI (CPI nanoemulsions) and NCPI-Dex Mix (CPI-Dex Mix nanoemulsions), the NCPI-Dex Con (CPI-Dex Con nanoemulsions) has better stability and flavor.
34848094	4	25	theme	gel	746:748	arg1	highest					788:794	highest	788:794	highest	788:794	The gel strength with 8% NCPI-Dex Con was the highest (5122.08 g‧mm), a 51.07% increase compared with the control group (3390.58 g‧mm).
34848094	4	25	theme	gel	746:748	arg1	strength					750:757	The gel strength	742:757	The gel strength with 8% NCPI-Dex Con	742:778	The gel strength with 8% NCPI-Dex Con was the highest (5122.08 g‧mm), a 51.07% increase compared with the control group (3390.58 g‧mm).
34848094	1	26	theme	particle	332:339	arg1	size					341:344	particle size	332:344	particle size	332:344	In this study, the stability and flavor characteristics of nanoemulsions prepared with dextran-conjugated Meretrix meretrix clam protein isolate were studied by characterizing particle size, polydispersity index, zeta potential, turbidity, microstructure, e-tongue, e-nose and HS-GC-IMS.
34848094	0	27	theme	protein	139:145	arg1	isolate					147:153	dextran-conjugated clam Meretrix meretrix linnaeus protein isolate	88:153	dextran-conjugated clam Meretrix meretrix linnaeus protein isolate	88:153	Preparation, characterization, and gel characteristics of nanoemulsions stabilized with dextran-conjugated clam Meretrix meretrix linnaeus protein isolate.
34848094	5	28	theme	uniform	995:1001	arg1	structure					1007:1015	the most uniform gel structure	986:1015	the most uniform gel structure	986:1015	The clam sausages supplemented with the 8% NCPI-Dex Con had the highest sensory score, with the densest and the most uniform gel structure.
34848094	2	29	contain	has	578:580	arg2	stability					589:597	stability	589:597	stability	589:597	Compared with the NCPI (CPI nanoemulsions) and NCPI-Dex Mix (CPI-Dex Mix nanoemulsions), the NCPI-Dex Con (CPI-Dex Con nanoemulsions) has better stability and flavor.
34848094	2	29	contain	has	578:580	arg1	Con					546:548	the NCPI-Dex Con	533:548	the NCPI-Dex Con (CPI-Dex Con nanoemulsions)	533:576	Compared with the NCPI (CPI nanoemulsions) and NCPI-Dex Mix (CPI-Dex Mix nanoemulsions), the NCPI-Dex Con (CPI-Dex Con nanoemulsions) has better stability and flavor.
34848094	2	29	contain	has	578:580	arg1	nanoemulsions					563:575	CPI-Dex Con nanoemulsions	551:575	CPI-Dex Con nanoemulsions	551:575	Compared with the NCPI (CPI nanoemulsions) and NCPI-Dex Mix (CPI-Dex Mix nanoemulsions), the NCPI-Dex Con (CPI-Dex Con nanoemulsions) has better stability and flavor.
34848094	2	29	contain	has	578:580	arg2	flavor					603:608	flavor	603:608	flavor	603:608	Compared with the NCPI (CPI nanoemulsions) and NCPI-Dex Mix (CPI-Dex Mix nanoemulsions), the NCPI-Dex Con (CPI-Dex Con nanoemulsions) has better stability and flavor.
34848094	1	30	theme	nanoemulsions	215:227	arg1	stability					175:183	stability	175:183	stability	175:183	In this study, the stability and flavor characteristics of nanoemulsions prepared with dextran-conjugated Meretrix meretrix clam protein isolate were studied by characterizing particle size, polydispersity index, zeta potential, turbidity, microstructure, e-tongue, e-nose and HS-GC-IMS.
34848094	1	30	theme	nanoemulsions	215:227	arg1	characteristics					196:210	flavor characteristics	189:210	flavor characteristics	189:210	In this study, the stability and flavor characteristics of nanoemulsions prepared with dextran-conjugated Meretrix meretrix clam protein isolate were studied by characterizing particle size, polydispersity index, zeta potential, turbidity, microstructure, e-tongue, e-nose and HS-GC-IMS.
34848094	0	31	theme	gel	35:37	arg1	characteristics					39:53	gel characteristics	35:53	gel characteristics	35:53	Preparation, characterization, and gel characteristics of nanoemulsions stabilized with dextran-conjugated clam Meretrix meretrix linnaeus protein isolate.
34848094	4	32	with	strength	750:757	arg1	Con					776:778	8% NCPI-Dex Con	764:778	8% NCPI-Dex Con	764:778	The gel strength with 8% NCPI-Dex Con was the highest (5122.08 g‧mm), a 51.07% increase compared with the control group (3390.58 g‧mm).
34848094	3	33	theme	clam	656:659	arg1	sausages					661:668	clam sausages	656:668	clam sausages	656:668	The breaking strength and breaking strain of clam sausages were significantly (P > 0.05) affected by the addition of NCPI-Dex Con.
34848094	6	34	theme	clam	1127:1130	arg1	sausages					1132:1139	the clam sausages	1123:1139	the clam sausages	1123:1139	Therefore, CPI-Dex Con stabilized nanoemulsions could effectively improve the gel property and flavor of the clam sausages.
34848094	3	35	dep	strength	624:631	arg1	The					611:613	The	611:613	The	611:613	The breaking strength and breaking strain of clam sausages were significantly (P > 0.05) affected by the addition of NCPI-Dex Con.
34848094	2	36	theme	NCPI-Dex	491:498	arg1	nanoemulsions					517:529	CPI-Dex Mix nanoemulsions	505:529	CPI-Dex Mix nanoemulsions	505:529	Compared with the NCPI (CPI nanoemulsions) and NCPI-Dex Mix (CPI-Dex Mix nanoemulsions), the NCPI-Dex Con (CPI-Dex Con nanoemulsions) has better stability and flavor.
34848094	2	36	theme	NCPI-Dex	491:498	arg1	Mix					500:502	NCPI-Dex Mix	491:502	NCPI-Dex Mix (CPI-Dex Mix nanoemulsions)	491:530	Compared with the NCPI (CPI nanoemulsions) and NCPI-Dex Mix (CPI-Dex Mix nanoemulsions), the NCPI-Dex Con (CPI-Dex Con nanoemulsions) has better stability and flavor.
34848094	3	37	theme	breaking	637:644	arg1	strain					646:651	breaking strain	637:651	breaking strain	637:651	The breaking strength and breaking strain of clam sausages were significantly (P > 0.05) affected by the addition of NCPI-Dex Con.
34848094	2	38	theme	NCPI-Dex	537:544	arg1	Con					546:548	the NCPI-Dex Con	533:548	the NCPI-Dex Con (CPI-Dex Con nanoemulsions)	533:576	Compared with the NCPI (CPI nanoemulsions) and NCPI-Dex Mix (CPI-Dex Mix nanoemulsions), the NCPI-Dex Con (CPI-Dex Con nanoemulsions) has better stability and flavor.
34848094	2	38	theme	NCPI-Dex	537:544	arg1	nanoemulsions					563:575	CPI-Dex Con nanoemulsions	551:575	CPI-Dex Con nanoemulsions	551:575	Compared with the NCPI (CPI nanoemulsions) and NCPI-Dex Mix (CPI-Dex Mix nanoemulsions), the NCPI-Dex Con (CPI-Dex Con nanoemulsions) has better stability and flavor.
34848094	1	39	theme	polydispersity	347:360	arg1	index					362:366	polydispersity index	347:366	polydispersity index	347:366	In this study, the stability and flavor characteristics of nanoemulsions prepared with dextran-conjugated Meretrix meretrix clam protein isolate were studied by characterizing particle size, polydispersity index, zeta potential, turbidity, microstructure, e-tongue, e-nose and HS-GC-IMS.
34848094	1	40	dep	Meretrix	262:269	arg1	meretrix					271:278	meretrix	271:278	meretrix	271:278	In this study, the stability and flavor characteristics of nanoemulsions prepared with dextran-conjugated Meretrix meretrix clam protein isolate were studied by characterizing particle size, polydispersity index, zeta potential, turbidity, microstructure, e-tongue, e-nose and HS-GC-IMS.
34848094	1	41	dep	stability	175:183	arg1	the					171:173	the	171:173	the	171:173	In this study, the stability and flavor characteristics of nanoemulsions prepared with dextran-conjugated Meretrix meretrix clam protein isolate were studied by characterizing particle size, polydispersity index, zeta potential, turbidity, microstructure, e-tongue, e-nose and HS-GC-IMS.
34848094	4	42	theme	control	848:854	arg1	3390.58 g‧mm					863:874	3390.58 g‧mm	863:874	3390.58 g‧mm	863:874	The gel strength with 8% NCPI-Dex Con was the highest (5122.08 g‧mm), a 51.07% increase compared with the control group (3390.58 g‧mm).
34848094	4	42	theme	control	848:854	arg1	group					856:860	the control group	844:860	the control group (3390.58 g‧mm)	844:875	The gel strength with 8% NCPI-Dex Con was the highest (5122.08 g‧mm), a 51.07% increase compared with the control group (3390.58 g‧mm).
34848094	5	43	theme	sensory	950:956	arg1	score					958:962	the highest sensory score	938:962	the highest sensory score	938:962	The clam sausages supplemented with the 8% NCPI-Dex Con had the highest sensory score, with the densest and the most uniform gel structure.
34848094	3	44	theme	Con	737:739	arg1	addition					716:723	the addition	712:723	the addition of NCPI-Dex Con	712:739	The breaking strength and breaking strain of clam sausages were significantly (P > 0.05) affected by the addition of NCPI-Dex Con.
34848094	1	45	theme	dextran-conjugated	243:260	arg1	isolate					293:299	dextran-conjugated Meretrix meretrix clam protein isolate	243:299	dextran-conjugated Meretrix meretrix clam protein isolate	243:299	In this study, the stability and flavor characteristics of nanoemulsions prepared with dextran-conjugated Meretrix meretrix clam protein isolate were studied by characterizing particle size, polydispersity index, zeta potential, turbidity, microstructure, e-tongue, e-nose and HS-GC-IMS.
34848094	3	46	theme	sausages	661:668	arg1	strength					624:631	breaking strength	615:631	breaking strength	615:631	The breaking strength and breaking strain of clam sausages were significantly (P > 0.05) affected by the addition of NCPI-Dex Con.
34848094	3	46	theme	sausages	661:668	arg1	strain					646:651	breaking strain	637:651	breaking strain	637:651	The breaking strength and breaking strain of clam sausages were significantly (P > 0.05) affected by the addition of NCPI-Dex Con.
34848094	4	47	theme	NCPI-Dex	767:774	arg1	Con					776:778	8% NCPI-Dex Con	764:778	8% NCPI-Dex Con	764:778	The gel strength with 8% NCPI-Dex Con was the highest (5122.08 g‧mm), a 51.07% increase compared with the control group (3390.58 g‧mm).
34848094	2	48	theme	Mix	513:515	arg1	nanoemulsions					517:529	CPI-Dex Mix nanoemulsions	505:529	CPI-Dex Mix nanoemulsions	505:529	Compared with the NCPI (CPI nanoemulsions) and NCPI-Dex Mix (CPI-Dex Mix nanoemulsions), the NCPI-Dex Con (CPI-Dex Con nanoemulsions) has better stability and flavor.
34848094	2	48	theme	Mix	513:515	arg1	Mix					500:502	NCPI-Dex Mix	491:502	NCPI-Dex Mix (CPI-Dex Mix nanoemulsions)	491:530	Compared with the NCPI (CPI nanoemulsions) and NCPI-Dex Mix (CPI-Dex Mix nanoemulsions), the NCPI-Dex Con (CPI-Dex Con nanoemulsions) has better stability and flavor.
34848094	1	49	theme	Meretrix	262:269	arg1	isolate					293:299	dextran-conjugated Meretrix meretrix clam protein isolate	243:299	dextran-conjugated Meretrix meretrix clam protein isolate	243:299	In this study, the stability and flavor characteristics of nanoemulsions prepared with dextran-conjugated Meretrix meretrix clam protein isolate were studied by characterizing particle size, polydispersity index, zeta potential, turbidity, microstructure, e-tongue, e-nose and HS-GC-IMS.
34848094	5	50	theme	NCPI-Dex	921:928	arg1	Con					930:932	the 8% NCPI-Dex Con	914:932	the 8% NCPI-Dex Con	914:932	The clam sausages supplemented with the 8% NCPI-Dex Con had the highest sensory score, with the densest and the most uniform gel structure.
34848094	5	51	theme	highest	942:948	arg1	score					958:962	the highest sensory score	938:962	the highest sensory score	938:962	The clam sausages supplemented with the 8% NCPI-Dex Con had the highest sensory score, with the densest and the most uniform gel structure.
34848094	6	52	theme	Con	1037:1039	arg1	nanoemulsions					1052:1064	CPI-Dex Con stabilized nanoemulsions	1029:1064	CPI-Dex Con stabilized nanoemulsions	1029:1064	Therefore, CPI-Dex Con stabilized nanoemulsions could effectively improve the gel property and flavor of the clam sausages.
34848094	0	53	theme	nanoemulsions	58:70	arg1	characterization					13:28	characterization	13:28	characterization	13:28	Preparation, characterization, and gel characteristics of nanoemulsions stabilized with dextran-conjugated clam Meretrix meretrix linnaeus protein isolate.
34848094	0	53	theme	nanoemulsions	58:70	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, characterization, and gel characteristics of nanoemulsions stabilized with dextran-conjugated clam Meretrix meretrix linnaeus protein isolate.
34848094	0	53	theme	nanoemulsions	58:70	arg1	characteristics					39:53	gel characteristics	35:53	gel characteristics	35:53	Preparation, characterization, and gel characteristics of nanoemulsions stabilized with dextran-conjugated clam Meretrix meretrix linnaeus protein isolate.
34848094	1	54	theme	zeta	369:372	arg1	potential					374:382	zeta potential	369:382	zeta potential	369:382	In this study, the stability and flavor characteristics of nanoemulsions prepared with dextran-conjugated Meretrix meretrix clam protein isolate were studied by characterizing particle size, polydispersity index, zeta potential, turbidity, microstructure, e-tongue, e-nose and HS-GC-IMS.
34848094	3	55	theme	breaking	615:622	arg1	strength					624:631	breaking strength	615:631	breaking strength	615:631	The breaking strength and breaking strain of clam sausages were significantly (P > 0.05) affected by the addition of NCPI-Dex Con.
34848094	1	56	theme	clam	280:283	arg1	isolate					293:299	dextran-conjugated Meretrix meretrix clam protein isolate	243:299	dextran-conjugated Meretrix meretrix clam protein isolate	243:299	In this study, the stability and flavor characteristics of nanoemulsions prepared with dextran-conjugated Meretrix meretrix clam protein isolate were studied by characterizing particle size, polydispersity index, zeta potential, turbidity, microstructure, e-tongue, e-nose and HS-GC-IMS.
32128969	0	0	theme	spring	96:101	arg1	phytoplankton					103:115	a spring phytoplankton	94:115	a spring phytoplankton	94:115	Short-term changes in polysaccharide utilization mechanisms of marine bacterioplankton during a spring phytoplankton bloom.
32128969	7	1	theme	external	1169:1176	arg1	rates					1189:1193	external hydrolysis rates	1169:1193	external hydrolysis rates	1169:1193	As the bloom progressed, selfish uptake increased markedly, and external hydrolysis rates increased, but only for a limited range of substrates.
32128969	9	2	theme	utilization	1438:1448	arg1	mode					1450:1453	Substrate utilization mode	1428:1453	Substrate utilization mode	1428:1453	Substrate utilization mode is related both to substrate structural complexity and to the bloom-stage dependent composition of the heterotrophic bacterial community.
32128969	8	3	theme	selfish	1371:1377	arg1	uptake					1379:1384	selfish uptake	1371:1384	selfish uptake of polysaccharides	1371:1403	The late bloom phase was characterized by high external hydrolysis rates of a broad range of polysaccharides and reduced selfish uptake of polysaccharides, except for laminarin.
32128969	6	4	theme	extracellular	1048:1060	arg1	rates					1073:1077	low extracellular hydrolysis rates	1044:1077	low extracellular hydrolysis rates of a few polysaccharides	1044:1102	In the early bloom phase, selfish activity was accompanied by low extracellular hydrolysis rates of a few polysaccharides.
32128969	8	5	theme	polysaccharides	1343:1357	arg1	range					1334:1338	a broad range	1326:1338	a broad range of polysaccharides	1326:1357	The late bloom phase was characterized by high external hydrolysis rates of a broad range of polysaccharides and reduced selfish uptake of polysaccharides, except for laminarin.
32128969	0	6	from	changes	11:17	arg1	mechanisms					49:58	polysaccharide utilization mechanisms	22:58	polysaccharide utilization mechanisms of marine bacterioplankton	22:85	Short-term changes in polysaccharide utilization mechanisms of marine bacterioplankton during a spring phytoplankton bloom.
32128969	7	7	theme	hydrolysis	1178:1187	arg1	rates					1189:1193	external hydrolysis rates	1169:1193	external hydrolysis rates	1169:1193	As the bloom progressed, selfish uptake increased markedly, and external hydrolysis rates increased, but only for a limited range of substrates.
32128969	4	8	theme	weight	727:732	arg1	products					740:747	no low-molecular weight (LMW) products	710:747	no low-molecular weight (LMW) products	710:747	Polysaccharides can be taken up in a 'selfish' mode, where initial hydrolysis is coupled to transport into the periplasm, such that little to no low-molecular weight (LMW) products are externally released to the environment.
32128969	6	9	theme	low	1044:1046	arg1	rates					1073:1077	low extracellular hydrolysis rates	1044:1077	low extracellular hydrolysis rates of a few polysaccharides	1044:1102	In the early bloom phase, selfish activity was accompanied by low extracellular hydrolysis rates of a few polysaccharides.
32128969	7	10	theme	substrates	1238:1247	arg1	range					1229:1233	a limited range	1219:1233	a limited range of substrates	1219:1247	As the bloom progressed, selfish uptake increased markedly, and external hydrolysis rates increased, but only for a limited range of substrates.
32128969	5	11	dep	attached	851:858	arg1	cell-surface					838:849	cell-surface	838:849	cell-surface	838:849	Alternatively, polysaccharides hydrolyzed by cell-surface attached or free extracellular enzymes (external hydrolysis) yield LMW products available to the wider bacterioplankton community.
32128969	6	12	theme	polysaccharides	1088:1102	arg1	rates					1073:1077	low extracellular hydrolysis rates	1044:1077	low extracellular hydrolysis rates of a few polysaccharides	1044:1102	In the early bloom phase, selfish activity was accompanied by low extracellular hydrolysis rates of a few polysaccharides.
32128969	3	13	theme	heterotrophic	467:479	arg1	bacteria					481:488	heterotrophic bacteria	467:488	heterotrophic bacteria	467:488	We tracked changes in the mode of polysaccharide utilization by heterotrophic bacteria during the course of a diatom-dominated bloom in the German Bight, North Sea.
32128969	9	14	theme	bacterial	1572:1580	arg1	community					1582:1590	the heterotrophic bacterial community	1554:1590	the heterotrophic bacterial community	1554:1590	Substrate utilization mode is related both to substrate structural complexity and to the bloom-stage dependent composition of the heterotrophic bacterial community.
32128969	4	15	theme	initial	627:633	arg1	hydrolysis					635:644	initial hydrolysis	627:644	initial hydrolysis	627:644	Polysaccharides can be taken up in a 'selfish' mode, where initial hydrolysis is coupled to transport into the periplasm, such that little to no low-molecular weight (LMW) products are externally released to the environment.
32128969	5	16	theme	bacterioplankton	954:969	arg1	community					971:979	the wider bacterioplankton community	944:979	the wider bacterioplankton community	944:979	Alternatively, polysaccharides hydrolyzed by cell-surface attached or free extracellular enzymes (external hydrolysis) yield LMW products available to the wider bacterioplankton community.
32128969	3	17	theme	German	543:548	arg1	North					557:561	North	557:561	North	557:561	We tracked changes in the mode of polysaccharide utilization by heterotrophic bacteria during the course of a diatom-dominated bloom in the German Bight, North Sea.
32128969	3	17	theme	German	543:548	arg1	Sea					563:565	the German Bight, North Sea	539:565	Sea	563:565	We tracked changes in the mode of polysaccharide utilization by heterotrophic bacteria during the course of a diatom-dominated bloom in the German Bight, North Sea.
32128969	0	18	dep	bloom	117:121	arg1	changes					11:17	Short-term changes	0:17	Short-term changes in polysaccharide utilization mechanisms of marine bacterioplankton during a spring phytoplankton	0:115	Short-term changes in polysaccharide utilization mechanisms of marine bacterioplankton during a spring phytoplankton bloom.
32128969	8	19	theme	high	1292:1295	arg1	rates					1317:1321	high external hydrolysis rates	1292:1321	high external hydrolysis rates of a broad range of polysaccharides	1292:1357	The late bloom phase was characterized by high external hydrolysis rates of a broad range of polysaccharides and reduced selfish uptake of polysaccharides, except for laminarin.
32128969	1	20	theme	global	211:216	arg1	productivity					225:236	global marine productivity	211:236	global marine productivity	211:236	Spring phytoplankton blooms in temperate environments contribute disproportionately to global marine productivity.
32128969	9	21	theme	substrate	1474:1482	arg1	complexity					1495:1504	substrate structural complexity	1474:1504	substrate structural complexity	1474:1504	Substrate utilization mode is related both to substrate structural complexity and to the bloom-stage dependent composition of the heterotrophic bacterial community.
32128969	5	22	theme	wider	948:952	arg1	community					971:979	the wider bacterioplankton community	944:979	the wider bacterioplankton community	944:979	Alternatively, polysaccharides hydrolyzed by cell-surface attached or free extracellular enzymes (external hydrolysis) yield LMW products available to the wider bacterioplankton community.
32128969	7	23	theme	limited	1221:1227	arg1	range					1229:1233	a limited range	1219:1233	a limited range of substrates	1219:1247	As the bloom progressed, selfish uptake increased markedly, and external hydrolysis rates increased, but only for a limited range of substrates.
32128969	8	24	theme	broad	1328:1332	arg1	range					1334:1338	a broad range	1326:1338	a broad range of polysaccharides	1326:1357	The late bloom phase was characterized by high external hydrolysis rates of a broad range of polysaccharides and reduced selfish uptake of polysaccharides, except for laminarin.
32128969	2	25	theme	biogeochemical	316:329	arg1	cycles					331:336	biogeochemical cycles	316:336	biogeochemical cycles driven by interacting autotrophic and heterotrophic communities	316:400	Bloom-derived organic matter, much of it occurring as polysaccharides, fuels biogeochemical cycles driven by interacting autotrophic and heterotrophic communities.
32128969	1	26	theme	marine	218:223	arg1	productivity					225:236	global marine productivity	211:236	global marine productivity	211:236	Spring phytoplankton blooms in temperate environments contribute disproportionately to global marine productivity.
32128969	3	27	theme	utilization	452:462	arg1	mode					429:432	the mode	425:432	the mode of polysaccharide utilization	425:462	We tracked changes in the mode of polysaccharide utilization by heterotrophic bacteria during the course of a diatom-dominated bloom in the German Bight, North Sea.
32128969	7	28	theme	selfish	1130:1136	arg1	uptake					1138:1143	selfish uptake	1130:1143	selfish uptake	1130:1143	As the bloom progressed, selfish uptake increased markedly, and external hydrolysis rates increased, but only for a limited range of substrates.
32128969	2	29	theme	heterotrophic	376:388	arg1	communities					390:400	autotrophic and heterotrophic communities	360:400	autotrophic and heterotrophic communities	360:400	Bloom-derived organic matter, much of it occurring as polysaccharides, fuels biogeochemical cycles driven by interacting autotrophic and heterotrophic communities.
32128969	5	30	theme	LMW	918:920	arg1	products					922:929	LMW products	918:929	LMW products available to the wider bacterioplankton community	918:979	Alternatively, polysaccharides hydrolyzed by cell-surface attached or free extracellular enzymes (external hydrolysis) yield LMW products available to the wider bacterioplankton community.
32128969	0	31	theme	Short-term	0:9	arg1	changes					11:17	Short-term changes	0:17	Short-term changes in polysaccharide utilization mechanisms of marine bacterioplankton during a spring phytoplankton	0:115	Short-term changes in polysaccharide utilization mechanisms of marine bacterioplankton during a spring phytoplankton bloom.
32128969	4	32	theme	selfish	606:612	arg1	mode					615:618	a 'selfish' mode	603:618	a 'selfish' mode	603:618	Polysaccharides can be taken up in a 'selfish' mode, where initial hydrolysis is coupled to transport into the periplasm, such that little to no low-molecular weight (LMW) products are externally released to the environment.
32128969	8	33	theme	external	1297:1304	arg1	rates					1317:1321	high external hydrolysis rates	1292:1321	high external hydrolysis rates of a broad range of polysaccharides	1292:1357	The late bloom phase was characterized by high external hydrolysis rates of a broad range of polysaccharides and reduced selfish uptake of polysaccharides, except for laminarin.
32128969	0	34	theme	polysaccharide	22:35	arg1	mechanisms					49:58	polysaccharide utilization mechanisms	22:58	polysaccharide utilization mechanisms of marine bacterioplankton	22:85	Short-term changes in polysaccharide utilization mechanisms of marine bacterioplankton during a spring phytoplankton bloom.
32128969	8	35	theme	range	1334:1338	arg1	rates					1317:1321	high external hydrolysis rates	1292:1321	high external hydrolysis rates of a broad range of polysaccharides	1292:1357	The late bloom phase was characterized by high external hydrolysis rates of a broad range of polysaccharides and reduced selfish uptake of polysaccharides, except for laminarin.
32128969	1	36	from	blooms	145:150	arg1	environments					165:176	temperate environments	155:176	temperate environments	155:176	Spring phytoplankton blooms in temperate environments contribute disproportionately to global marine productivity.
32128969	2	37	theme	autotrophic	360:370	arg1	communities					390:400	autotrophic and heterotrophic communities	360:400	autotrophic and heterotrophic communities	360:400	Bloom-derived organic matter, much of it occurring as polysaccharides, fuels biogeochemical cycles driven by interacting autotrophic and heterotrophic communities.
32128969	8	38	theme	hydrolysis	1306:1315	arg1	rates					1317:1321	high external hydrolysis rates	1292:1321	high external hydrolysis rates of a broad range of polysaccharides	1292:1357	The late bloom phase was characterized by high external hydrolysis rates of a broad range of polysaccharides and reduced selfish uptake of polysaccharides, except for laminarin.
32128969	3	39	theme	polysaccharide	437:450	arg1	utilization					452:462	polysaccharide utilization	437:462	polysaccharide utilization	437:462	We tracked changes in the mode of polysaccharide utilization by heterotrophic bacteria during the course of a diatom-dominated bloom in the German Bight, North Sea.
32128969	6	40	theme	selfish	1008:1014	arg1	activity					1016:1023	selfish activity	1008:1023	selfish activity	1008:1023	In the early bloom phase, selfish activity was accompanied by low extracellular hydrolysis rates of a few polysaccharides.
32128969	9	41	theme	heterotrophic	1558:1570	arg1	community					1582:1590	the heterotrophic bacterial community	1554:1590	the heterotrophic bacterial community	1554:1590	Substrate utilization mode is related both to substrate structural complexity and to the bloom-stage dependent composition of the heterotrophic bacterial community.
32128969	3	42	from	changes	414:420	arg1	mode					429:432	the mode	425:432	the mode of polysaccharide utilization	425:462	We tracked changes in the mode of polysaccharide utilization by heterotrophic bacteria during the course of a diatom-dominated bloom in the German Bight, North Sea.
32128969	1	43	theme	Spring	124:129	arg1	blooms					145:150	Spring phytoplankton blooms	124:150	Spring phytoplankton blooms in temperate environments	124:176	Spring phytoplankton blooms in temperate environments contribute disproportionately to global marine productivity.
32128969	2	44	link	Bloom-derived	239:251	arg1	matter					261:266	Bloom-derived organic matter	239:266	Bloom-derived organic matter	239:266	Bloom-derived organic matter, much of it occurring as polysaccharides, fuels biogeochemical cycles driven by interacting autotrophic and heterotrophic communities.
32128969	8	45	theme	late	1254:1257	arg1	phase					1265:1269	The late bloom phase	1250:1269	The late bloom phase	1250:1269	The late bloom phase was characterized by high external hydrolysis rates of a broad range of polysaccharides and reduced selfish uptake of polysaccharides, except for laminarin.
32128969	5	46	theme	external	891:898	arg1	enzymes					882:888	cell-surface attached or free extracellular enzymes	838:888	cell-surface attached or free extracellular enzymes (external hydrolysis)	838:910	Alternatively, polysaccharides hydrolyzed by cell-surface attached or free extracellular enzymes (external hydrolysis) yield LMW products available to the wider bacterioplankton community.
32128969	5	46	theme	external	891:898	arg1	hydrolysis					900:909	external hydrolysis	891:909	external hydrolysis	891:909	Alternatively, polysaccharides hydrolyzed by cell-surface attached or free extracellular enzymes (external hydrolysis) yield LMW products available to the wider bacterioplankton community.
32128969	3	47	theme	bloom	530:534	arg1	course					501:506	the course	497:506	the course of a diatom-dominated bloom in the German Bight, North Sea	497:565	We tracked changes in the mode of polysaccharide utilization by heterotrophic bacteria during the course of a diatom-dominated bloom in the German Bight, North Sea.
32128969	0	48	theme	utilization	37:47	arg1	mechanisms					49:58	polysaccharide utilization mechanisms	22:58	polysaccharide utilization mechanisms of marine bacterioplankton	22:85	Short-term changes in polysaccharide utilization mechanisms of marine bacterioplankton during a spring phytoplankton bloom.
32128969	7	49	theme	bloom	1112:1116	arg1	progressed					1118:1127	the bloom progressed	1108:1127	the bloom progressed	1108:1127	As the bloom progressed, selfish uptake increased markedly, and external hydrolysis rates increased, but only for a limited range of substrates.
32128969	5	50	theme	free	863:866	arg1	enzymes					882:888	cell-surface attached or free extracellular enzymes	838:888	cell-surface attached or free extracellular enzymes (external hydrolysis)	838:910	Alternatively, polysaccharides hydrolyzed by cell-surface attached or free extracellular enzymes (external hydrolysis) yield LMW products available to the wider bacterioplankton community.
32128969	5	50	theme	free	863:866	arg1	hydrolysis					900:909	external hydrolysis	891:909	external hydrolysis	891:909	Alternatively, polysaccharides hydrolyzed by cell-surface attached or free extracellular enzymes (external hydrolysis) yield LMW products available to the wider bacterioplankton community.
32128969	6	51	theme	few	1084:1086	arg1	polysaccharides					1088:1102	a few polysaccharides	1082:1102	a few polysaccharides	1082:1102	In the early bloom phase, selfish activity was accompanied by low extracellular hydrolysis rates of a few polysaccharides.
32128969	0	52	theme	marine	63:68	arg1	bacterioplankton					70:85	marine bacterioplankton	63:85	marine bacterioplankton	63:85	Short-term changes in polysaccharide utilization mechanisms of marine bacterioplankton during a spring phytoplankton bloom.
32128969	5	53	theme	available	931:939	arg1	products					922:929	LMW products	918:929	LMW products available to the wider bacterioplankton community	918:979	Alternatively, polysaccharides hydrolyzed by cell-surface attached or free extracellular enzymes (external hydrolysis) yield LMW products available to the wider bacterioplankton community.
32128969	9	54	theme	dependent	1529:1537	arg1	composition					1539:1549	the bloom-stage dependent composition	1513:1549	the bloom-stage dependent composition of the heterotrophic bacterial community	1513:1590	Substrate utilization mode is related both to substrate structural complexity and to the bloom-stage dependent composition of the heterotrophic bacterial community.
32128969	9	55	theme	structural	1484:1493	arg1	complexity					1495:1504	substrate structural complexity	1474:1504	substrate structural complexity	1474:1504	Substrate utilization mode is related both to substrate structural complexity and to the bloom-stage dependent composition of the heterotrophic bacterial community.
32128969	1	56	theme	phytoplankton	131:143	arg1	blooms					145:150	Spring phytoplankton blooms	124:150	Spring phytoplankton blooms in temperate environments	124:176	Spring phytoplankton blooms in temperate environments contribute disproportionately to global marine productivity.
32128969	8	57	theme	bloom	1259:1263	arg1	phase					1265:1269	The late bloom phase	1250:1269	The late bloom phase	1250:1269	The late bloom phase was characterized by high external hydrolysis rates of a broad range of polysaccharides and reduced selfish uptake of polysaccharides, except for laminarin.
32128969	6	58	theme	early	989:993	arg1	phase					1001:1005	the early bloom phase	985:1005	the early bloom phase	985:1005	In the early bloom phase, selfish activity was accompanied by low extracellular hydrolysis rates of a few polysaccharides.
32128969	3	59	from	Sea	563:565	arg1	course					501:506	the course	497:506	the course of a diatom-dominated bloom in the German Bight, North Sea	497:565	We tracked changes in the mode of polysaccharide utilization by heterotrophic bacteria during the course of a diatom-dominated bloom in the German Bight, North Sea.
32128969	3	60	theme	Bight	550:554	arg1	North					557:561	North	557:561	North	557:561	We tracked changes in the mode of polysaccharide utilization by heterotrophic bacteria during the course of a diatom-dominated bloom in the German Bight, North Sea.
32128969	3	60	theme	Bight	550:554	arg1	Sea					563:565	the German Bight, North Sea	539:565	Sea	563:565	We tracked changes in the mode of polysaccharide utilization by heterotrophic bacteria during the course of a diatom-dominated bloom in the German Bight, North Sea.
32128969	9	61	theme	Substrate	1428:1436	arg1	utilization					1438:1448	Substrate utilization	1428:1448	Substrate utilization mode	1428:1453	Substrate utilization mode is related both to substrate structural complexity and to the bloom-stage dependent composition of the heterotrophic bacterial community.
32128969	8	62	theme	polysaccharides	1389:1403	arg1	uptake					1379:1384	selfish uptake	1371:1384	selfish uptake of polysaccharides	1371:1403	The late bloom phase was characterized by high external hydrolysis rates of a broad range of polysaccharides and reduced selfish uptake of polysaccharides, except for laminarin.
32128969	2	63	theme	organic	253:259	arg1	matter					261:266	Bloom-derived organic matter	239:266	Bloom-derived organic matter	239:266	Bloom-derived organic matter, much of it occurring as polysaccharides, fuels biogeochemical cycles driven by interacting autotrophic and heterotrophic communities.
32128969	3	64	from	course	501:506	arg1	North					557:561	North	557:561	North	557:561	We tracked changes in the mode of polysaccharide utilization by heterotrophic bacteria during the course of a diatom-dominated bloom in the German Bight, North Sea.
32128969	3	64	from	course	501:506	arg1	Sea					563:565	the German Bight, North Sea	539:565	Sea	563:565	We tracked changes in the mode of polysaccharide utilization by heterotrophic bacteria during the course of a diatom-dominated bloom in the German Bight, North Sea.
32128969	1	65	theme	temperate	155:163	arg1	environments					165:176	temperate environments	155:176	temperate environments	155:176	Spring phytoplankton blooms in temperate environments contribute disproportionately to global marine productivity.
32128969	4	66	dep	released	764:771	arg1	such					690:693	such	690:693	such	690:693	Polysaccharides can be taken up in a 'selfish' mode, where initial hydrolysis is coupled to transport into the periplasm, such that little to no low-molecular weight (LMW) products are externally released to the environment.
32128969	2	67	theme	Bloom-derived	239:251	arg1	matter					261:266	Bloom-derived organic matter	239:266	Bloom-derived organic matter	239:266	Bloom-derived organic matter, much of it occurring as polysaccharides, fuels biogeochemical cycles driven by interacting autotrophic and heterotrophic communities.
32128969	9	68	theme	community	1582:1590	arg1	composition					1539:1549	the bloom-stage dependent composition	1513:1549	the bloom-stage dependent composition of the heterotrophic bacterial community	1513:1590	Substrate utilization mode is related both to substrate structural complexity and to the bloom-stage dependent composition of the heterotrophic bacterial community.
32128969	4	69	theme	low-molecular	713:725	arg1	weight					727:732	no low-molecular weight	710:732	no low-molecular weight (LMW) products	710:747	Polysaccharides can be taken up in a 'selfish' mode, where initial hydrolysis is coupled to transport into the periplasm, such that little to no low-molecular weight (LMW) products are externally released to the environment.
32128969	4	69	theme	low-molecular	713:725	arg1	LMW					735:737	LMW	735:737	LMW	735:737	Polysaccharides can be taken up in a 'selfish' mode, where initial hydrolysis is coupled to transport into the periplasm, such that little to no low-molecular weight (LMW) products are externally released to the environment.
32128969	0	70	theme	bacterioplankton	70:85	arg1	mechanisms					49:58	polysaccharide utilization mechanisms	22:58	polysaccharide utilization mechanisms of marine bacterioplankton	22:85	Short-term changes in polysaccharide utilization mechanisms of marine bacterioplankton during a spring phytoplankton bloom.
32128969	5	71	theme	extracellular	868:880	arg1	enzymes					882:888	cell-surface attached or free extracellular enzymes	838:888	cell-surface attached or free extracellular enzymes (external hydrolysis)	838:910	Alternatively, polysaccharides hydrolyzed by cell-surface attached or free extracellular enzymes (external hydrolysis) yield LMW products available to the wider bacterioplankton community.
32128969	5	71	theme	extracellular	868:880	arg1	hydrolysis					900:909	external hydrolysis	891:909	external hydrolysis	891:909	Alternatively, polysaccharides hydrolyzed by cell-surface attached or free extracellular enzymes (external hydrolysis) yield LMW products available to the wider bacterioplankton community.
32128969	5	72	theme	attached	851:858	arg1	enzymes					882:888	cell-surface attached or free extracellular enzymes	838:888	cell-surface attached or free extracellular enzymes (external hydrolysis)	838:910	Alternatively, polysaccharides hydrolyzed by cell-surface attached or free extracellular enzymes (external hydrolysis) yield LMW products available to the wider bacterioplankton community.
32128969	5	72	theme	attached	851:858	arg1	hydrolysis					900:909	external hydrolysis	891:909	external hydrolysis	891:909	Alternatively, polysaccharides hydrolyzed by cell-surface attached or free extracellular enzymes (external hydrolysis) yield LMW products available to the wider bacterioplankton community.
32128969	6	73	theme	bloom	995:999	arg1	phase					1001:1005	the early bloom phase	985:1005	the early bloom phase	985:1005	In the early bloom phase, selfish activity was accompanied by low extracellular hydrolysis rates of a few polysaccharides.
32128969	6	74	theme	hydrolysis	1062:1071	arg1	rates					1073:1077	low extracellular hydrolysis rates	1044:1077	low extracellular hydrolysis rates of a few polysaccharides	1044:1102	In the early bloom phase, selfish activity was accompanied by low extracellular hydrolysis rates of a few polysaccharides.
33013929	5	0	from	epitopes	1064:1071	arg1	protein					1088:1094	the viral S protein	1076:1094	the viral S protein	1076:1094	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	3	1	theme	whole	686:690	arg1	sequences					699:707	8,897 whole genome sequences	680:707	8,897 whole genome sequences	680:707	We analyzed totally 7,813 sequences screened from 8,897 whole genome sequences on GISAID database up to April 26, and 18 S protein amino acid variations with relatively high frequency (≥10-3) were identified.
33013929	6	2	theme	glycan	1427:1432	arg1	sites					1434:1438	the glycan sites	1423:1438	the glycan sites	1423:1438	In addition, we detected 9 predicted N-glycosylation sites and 3 O-glycosylation sites unique to SARS-CoV-2, but no evidently observed variation of the glycan sites so far.
33013929	7	3	gly	glycosylation	1577:1589	arg2	sites					1591:1595	glycosylation sites	1577:1595	glycosylation sites	1577:1595	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	7	3	gly	glycosylation	1577:1589	arg2	basis					1626:1630	an essential basis	1613:1630	an essential basis for the selection of vaccine candidates	1613:1670	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	7	3	gly	glycosylation	1577:1589	arg2	epitopes					1564:1571	SARS-CoV-2 S protein cell epitopes	1538:1571	SARS-CoV-2 S protein cell epitopes	1538:1571	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	1	4	theme	acute	181:185	arg1	coronavirus					208:218	severe acute respiratory syndrome coronavirus 2	174:220	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	174:233	Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to an outbreak of a pandemic worldwide.
33013929	1	4	theme	acute	181:185	arg1	SARS-CoV-2					223:232	SARS-CoV-2	223:232	SARS-CoV-2	223:232	Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to an outbreak of a pandemic worldwide.
33013929	7	5	theme	S	1549:1549	arg1	epitopes					1564:1571	SARS-CoV-2 S protein cell epitopes	1538:1571	SARS-CoV-2 S protein cell epitopes	1538:1571	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	7	5	theme	S	1549:1549	arg1	sites					1591:1595	glycosylation sites	1577:1595	glycosylation sites	1577:1595	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	7	5	theme	S	1549:1549	arg1	basis					1626:1630	an essential basis	1613:1630	an essential basis for the selection of vaccine candidates	1613:1670	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	7	6	theme	vaccine	1653:1659	arg1	candidates					1661:1670	vaccine candidates	1653:1670	vaccine candidates	1653:1670	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	3	7	theme	GISAID	712:717	arg1	up					728:729	GISAID database up	712:729	GISAID database up to April 26	712:741	We analyzed totally 7,813 sequences screened from 8,897 whole genome sequences on GISAID database up to April 26, and 18 S protein amino acid variations with relatively high frequency (≥10-3) were identified.
33013929	5	8	theme	cell	985:988	arg1	epitopes					990:997	69 linear B cell epitopes	973:997	69 linear B cell epitopes	973:997	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	6	9	theme	observed	1401:1408	arg1	variation					1410:1418	no evidently observed variation	1388:1418	no evidently observed variation of the glycan sites	1388:1438	In addition, we detected 9 predicted N-glycosylation sites and 3 O-glycosylation sites unique to SARS-CoV-2, but no evidently observed variation of the glycan sites so far.
33013929	1	10	theme	syndrome	199:206	arg1	coronavirus					208:218	severe acute respiratory syndrome coronavirus 2	174:220	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	174:233	Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to an outbreak of a pandemic worldwide.
33013929	1	10	theme	syndrome	199:206	arg1	SARS-CoV-2					223:232	SARS-CoV-2	223:232	SARS-CoV-2	223:232	Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to an outbreak of a pandemic worldwide.
33013929	7	11	theme	geographical	1508:1519	arg1	distributions					1521:1533	temporal and geographical distributions	1495:1533	temporal and geographical distributions	1495:1533	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	2	12	theme	transmission	531:542	arg1	course					544:549	the global transmission course	520:549	the global transmission course	520:549	For better understanding the viral spike (S) protein variations and its potential effects on the interaction with the host immune system and also in vaccine development, the cell epitopes, glycosylation profile and their changes during the global transmission course were characterized and compared with SARS-CoV for their glycosylation profile.
33013929	5	13	dep	structure	1123:1131	arg1	the					1111:1113	the	1111:1113	the	1111:1113	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	5	14	theme	predicted	963:971	arg1	epitopes					990:997	69 linear B cell epitopes	973:997	69 linear B cell epitopes	973:997	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	1	15	theme	Coronavirus	127:137	arg1	disease					139:145	Coronavirus disease 2019	127:150	Coronavirus disease 2019 (COVID-19)	127:161	Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to an outbreak of a pandemic worldwide.
33013929	1	15	theme	Coronavirus	127:137	arg1	COVID-19					153:160	COVID-19	153:160	COVID-19	153:160	Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to an outbreak of a pandemic worldwide.
33013929	7	16	theme	temporal	1495:1502	arg1	distributions					1521:1533	temporal and geographical distributions	1495:1533	temporal and geographical distributions	1495:1533	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	4	17	theme	228	850:852	arg1	sequences					854:862	228 sequences	850:862	228 sequences of variants	850:874	A total of 228 sequences of variants had multiple variations, of note, most of them harboring the D614G mutation.
33013929	3	18	theme	protein	753:759	arg1	variations					772:781	18 S protein amino acid variations	748:781	18 S protein amino acid variations with relatively high frequency (≥10-3)	748:820	We analyzed totally 7,813 sequences screened from 8,897 whole genome sequences on GISAID database up to April 26, and 18 S protein amino acid variations with relatively high frequency (≥10-3) were identified.
33013929	5	19	theme	linear	976:981	arg1	epitopes					990:997	69 linear B cell epitopes	973:997	69 linear B cell epitopes	973:997	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	3	20	theme	high	799:802	arg1	≥10-3					815:819	≥10-3	815:819	≥10-3	815:819	We analyzed totally 7,813 sequences screened from 8,897 whole genome sequences on GISAID database up to April 26, and 18 S protein amino acid variations with relatively high frequency (≥10-3) were identified.
33013929	3	20	theme	high	799:802	arg1	frequency					804:812	relatively high frequency	788:812	relatively high frequency (≥10-3)	788:820	We analyzed totally 7,813 sequences screened from 8,897 whole genome sequences on GISAID database up to April 26, and 18 S protein amino acid variations with relatively high frequency (≥10-3) were identified.
33013929	3	21	theme	18	748:749	arg1	S					751:751	S	751:751	S	751:751	We analyzed totally 7,813 sequences screened from 8,897 whole genome sequences on GISAID database up to April 26, and 18 S protein amino acid variations with relatively high frequency (≥10-3) were identified.
33013929	0	22	theme	Glycosylation	57:69	arg1	Profiles					71:78	Glycosylation Profiles	57:78	Glycosylation Profiles	57:78	Variations in SARS-CoV-2 Spike Protein Cell Epitopes and Glycosylation Profiles During Global Transmission Course of COVID-19.
33013929	0	23	theme	SARS-CoV-2	14:23	arg1	Epitopes					44:51	SARS-CoV-2 Spike Protein Cell Epitopes	14:51	SARS-CoV-2 Spike Protein Cell Epitopes	14:51	Variations in SARS-CoV-2 Spike Protein Cell Epitopes and Glycosylation Profiles During Global Transmission Course of COVID-19.
33013929	5	24	theme	cytotoxic	1041:1049	arg1	lymphocyte					1053:1062	41 cytotoxic T lymphocyte	1038:1062	41 cytotoxic T lymphocyte epitopes	1038:1071	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	2	25	theme	glycosylation	473:485	arg1	profile					487:493	glycosylation profile	473:493	glycosylation profile	473:493	For better understanding the viral spike (S) protein variations and its potential effects on the interaction with the host immune system and also in vaccine development, the cell epitopes, glycosylation profile and their changes during the global transmission course were characterized and compared with SARS-CoV for their glycosylation profile.
33013929	0	26	from	Variations	0:9	arg1	Epitopes					44:51	SARS-CoV-2 Spike Protein Cell Epitopes	14:51	SARS-CoV-2 Spike Protein Cell Epitopes	14:51	Variations in SARS-CoV-2 Spike Protein Cell Epitopes and Glycosylation Profiles During Global Transmission Course of COVID-19.
33013929	0	26	from	Variations	0:9	arg1	Profiles					71:78	Glycosylation Profiles	57:78	Glycosylation Profiles	57:78	Variations in SARS-CoV-2 Spike Protein Cell Epitopes and Glycosylation Profiles During Global Transmission Course of COVID-19.
33013929	3	27	theme	acid	767:770	arg1	variations					772:781	18 S protein amino acid variations	748:781	18 S protein amino acid variations with relatively high frequency (≥10-3)	748:820	We analyzed totally 7,813 sequences screened from 8,897 whole genome sequences on GISAID database up to April 26, and 18 S protein amino acid variations with relatively high frequency (≥10-3) were identified.
33013929	0	28	theme	Transmission	94:105	arg1	Course					107:112	Global Transmission Course	87:112	Global Transmission Course of COVID-19	87:124	Variations in SARS-CoV-2 Spike Protein Cell Epitopes and Glycosylation Profiles During Global Transmission Course of COVID-19.
33013929	5	29	theme	discontinuous	1004:1016	arg1	epitopes					1025:1032	175 discontinuous B cell epitopes	1000:1032	175 discontinuous B cell epitopes	1000:1032	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	2	30	theme	potential	356:364	arg1	effects					366:372	its potential effects	352:372	its potential effects on the interaction with the host immune system	352:419	For better understanding the viral spike (S) protein variations and its potential effects on the interaction with the host immune system and also in vaccine development, the cell epitopes, glycosylation profile and their changes during the global transmission course were characterized and compared with SARS-CoV for their glycosylation profile.
33013929	5	31	from	epitopes	990:997	arg1	protein					1088:1094	the viral S protein	1076:1094	the viral S protein	1076:1094	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	5	32	theme	cell	1020:1023	arg1	epitopes					1025:1032	175 discontinuous B cell epitopes	1000:1032	175 discontinuous B cell epitopes	1000:1032	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	0	33	theme	Cell	39:42	arg1	Epitopes					44:51	SARS-CoV-2 Spike Protein Cell Epitopes	14:51	SARS-CoV-2 Spike Protein Cell Epitopes	14:51	Variations in SARS-CoV-2 Spike Protein Cell Epitopes and Glycosylation Profiles During Global Transmission Course of COVID-19.
33013929	7	34	theme	glycosylation	1577:1589	arg1	epitopes					1564:1571	SARS-CoV-2 S protein cell epitopes	1538:1571	SARS-CoV-2 S protein cell epitopes	1538:1571	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	7	34	theme	glycosylation	1577:1589	arg1	sites					1591:1595	glycosylation sites	1577:1595	glycosylation sites	1577:1595	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	7	34	theme	glycosylation	1577:1589	arg1	basis					1626:1630	an essential basis	1613:1630	an essential basis for the selection of vaccine candidates	1613:1670	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	2	35	theme	immune	407:412	arg1	system					414:419	the host immune system	398:419	the host immune system	398:419	For better understanding the viral spike (S) protein variations and its potential effects on the interaction with the host immune system and also in vaccine development, the cell epitopes, glycosylation profile and their changes during the global transmission course were characterized and compared with SARS-CoV for their glycosylation profile.
33013929	5	36	theme	discontinuous	1231:1243	arg1	epitope					1245:1251	discontinuous epitope	1231:1251	discontinuous epitope	1231:1251	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	5	37	from	epitopes	1025:1032	arg1	protein					1088:1094	the viral S protein	1076:1094	the viral S protein	1076:1094	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	5	38	theme	viral	1080:1084	arg1	protein					1088:1094	the viral S protein	1076:1094	the viral S protein	1076:1094	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	2	39	dep	spike	319:323	arg1	S					326:326	S	326:326	S	326:326	For better understanding the viral spike (S) protein variations and its potential effects on the interaction with the host immune system and also in vaccine development, the cell epitopes, glycosylation profile and their changes during the global transmission course were characterized and compared with SARS-CoV for their glycosylation profile.
33013929	6	40	theme	N-glycosylation	1312:1326	arg1	sites					1328:1332	predicted N-glycosylation sites	1302:1332	predicted N-glycosylation sites	1302:1332	In addition, we detected 9 predicted N-glycosylation sites and 3 O-glycosylation sites unique to SARS-CoV-2, but no evidently observed variation of the glycan sites so far.
33013929	7	41	theme	protein	1551:1557	arg1	epitopes					1564:1571	SARS-CoV-2 S protein cell epitopes	1538:1571	SARS-CoV-2 S protein cell epitopes	1538:1571	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	7	41	theme	protein	1551:1557	arg1	sites					1591:1595	glycosylation sites	1577:1595	glycosylation sites	1577:1595	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	7	41	theme	protein	1551:1557	arg1	basis					1626:1630	an essential basis	1613:1630	an essential basis for the selection of vaccine candidates	1613:1670	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	5	42	theme	epitope	1202:1208	arg1	length					1210:1215	the linear epitope length	1191:1215	the linear epitope length	1191:1215	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	0	43	theme	Protein	31:37	arg1	Epitopes					44:51	SARS-CoV-2 Spike Protein Cell Epitopes	14:51	SARS-CoV-2 Spike Protein Cell Epitopes	14:51	Variations in SARS-CoV-2 Spike Protein Cell Epitopes and Glycosylation Profiles During Global Transmission Course of COVID-19.
33013929	1	44	theme	worldwide	273:281	arg1	outbreak					250:257	an outbreak	247:257	an outbreak of a pandemic worldwide	247:281	Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to an outbreak of a pandemic worldwide.
33013929	4	45	theme	D614G	937:941	arg1	mutation					943:950	the D614G mutation	933:950	the D614G mutation	933:950	A total of 228 sequences of variants had multiple variations, of note, most of them harboring the D614G mutation.
33013929	5	46	theme	lymphocyte	1053:1062	arg1	epitopes					1064:1071	41 cytotoxic T lymphocyte epitopes	1038:1071	41 cytotoxic T lymphocyte epitopes	1038:1071	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	2	47	theme	spike	319:323	arg1	protein					329:335	the viral spike (S) protein	309:335	the viral spike (S) protein variations	309:346	For better understanding the viral spike (S) protein variations and its potential effects on the interaction with the host immune system and also in vaccine development, the cell epitopes, glycosylation profile and their changes during the global transmission course were characterized and compared with SARS-CoV for their glycosylation profile.
33013929	3	48	theme	7,813	650:654	arg1	sequences					656:664	7,813 sequences	650:664	7,813 sequences screened from 8,897 whole genome sequences on GISAID database up to April 26	650:741	We analyzed totally 7,813 sequences screened from 8,897 whole genome sequences on GISAID database up to April 26, and 18 S protein amino acid variations with relatively high frequency (≥10-3) were identified.
33013929	5	49	theme	protein	1115:1121	arg1	structure					1123:1131	protein structure	1115:1131	protein structure	1115:1131	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	7	50	from	snapshot	1483:1490	arg1	epitopes					1564:1571	SARS-CoV-2 S protein cell epitopes	1538:1571	SARS-CoV-2 S protein cell epitopes	1538:1571	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	7	50	from	snapshot	1483:1490	arg1	sites					1591:1595	glycosylation sites	1577:1595	glycosylation sites	1577:1595	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	7	50	from	snapshot	1483:1490	arg1	basis					1626:1630	an essential basis	1613:1630	an essential basis for the selection of vaccine candidates	1613:1670	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	2	51	theme	protein	329:335	arg1	variations					337:346	the viral spike (S) protein variations	309:346	the viral spike (S) protein variations	309:346	For better understanding the viral spike (S) protein variations and its potential effects on the interaction with the host immune system and also in vaccine development, the cell epitopes, glycosylation profile and their changes during the global transmission course were characterized and compared with SARS-CoV for their glycosylation profile.
33013929	6	52	theme	unique	1362:1367	arg1	sites					1328:1332	predicted N-glycosylation sites	1302:1332	predicted N-glycosylation sites	1302:1332	In addition, we detected 9 predicted N-glycosylation sites and 3 O-glycosylation sites unique to SARS-CoV-2, but no evidently observed variation of the glycan sites so far.
33013929	6	53	theme	O-glycosylation	1340:1354	arg1	sites					1356:1360	3 O-glycosylation sites	1338:1360	3 O-glycosylation sites	1338:1360	In addition, we detected 9 predicted N-glycosylation sites and 3 O-glycosylation sites unique to SARS-CoV-2, but no evidently observed variation of the glycan sites so far.
33013929	6	54	theme	sites	1434:1438	arg1	variation					1410:1418	no evidently observed variation	1388:1418	no evidently observed variation of the glycan sites	1388:1438	In addition, we detected 9 predicted N-glycosylation sites and 3 O-glycosylation sites unique to SARS-CoV-2, but no evidently observed variation of the glycan sites so far.
33013929	2	55	with	interaction	381:391	arg1	system					414:419	the host immune system	398:419	the host immune system	398:419	For better understanding the viral spike (S) protein variations and its potential effects on the interaction with the host immune system and also in vaccine development, the cell epitopes, glycosylation profile and their changes during the global transmission course were characterized and compared with SARS-CoV for their glycosylation profile.
33013929	7	56	theme	candidates	1661:1670	arg1	selection					1640:1648	the selection	1636:1648	the selection of vaccine candidates	1636:1670	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	3	57	theme	genome	692:697	arg1	sequences					699:707	8,897 whole genome sequences	680:707	8,897 whole genome sequences	680:707	We analyzed totally 7,813 sequences screened from 8,897 whole genome sequences on GISAID database up to April 26, and 18 S protein amino acid variations with relatively high frequency (≥10-3) were identified.
33013929	1	58	theme	severe	174:179	arg1	coronavirus					208:218	severe acute respiratory syndrome coronavirus 2	174:220	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	174:233	Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to an outbreak of a pandemic worldwide.
33013929	1	58	theme	severe	174:179	arg1	SARS-CoV-2					223:232	SARS-CoV-2	223:232	SARS-CoV-2	223:232	Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to an outbreak of a pandemic worldwide.
33013929	6	59	gly	O-glycosylation	1340:1354	arg2	3					1338:1338	3	1338:1338	3	1338:1338	In addition, we detected 9 predicted N-glycosylation sites and 3 O-glycosylation sites unique to SARS-CoV-2, but no evidently observed variation of the glycan sites so far.
33013929	6	59	gly	O-glycosylation	1340:1354	arg2	sites					1356:1360	3 O-glycosylation sites	1338:1360	3 O-glycosylation sites	1338:1360	In addition, we detected 9 predicted N-glycosylation sites and 3 O-glycosylation sites unique to SARS-CoV-2, but no evidently observed variation of the glycan sites so far.
33013929	7	60	theme	SARS-CoV-2	1538:1547	arg1	epitopes					1564:1571	SARS-CoV-2 S protein cell epitopes	1538:1571	SARS-CoV-2 S protein cell epitopes	1538:1571	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	7	60	theme	SARS-CoV-2	1538:1547	arg1	sites					1591:1595	glycosylation sites	1577:1595	glycosylation sites	1577:1595	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	7	60	theme	SARS-CoV-2	1538:1547	arg1	basis					1626:1630	an essential basis	1613:1630	an essential basis for the selection of vaccine candidates	1613:1670	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	1	61	theme	respiratory	187:197	arg1	coronavirus					208:218	severe acute respiratory syndrome coronavirus 2	174:220	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	174:233	Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to an outbreak of a pandemic worldwide.
33013929	1	61	theme	respiratory	187:197	arg1	SARS-CoV-2					223:232	SARS-CoV-2	223:232	SARS-CoV-2	223:232	Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to an outbreak of a pandemic worldwide.
33013929	7	62	theme	distributions	1521:1533	arg1	snapshot					1483:1490	an important snapshot	1470:1490	an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates	1470:1670	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	4	63	theme	sequences	854:862	arg1	total					841:845	A total	839:845	A total of 228 sequences of variants	839:874	A total of 228 sequences of variants had multiple variations, of note, most of them harboring the D614G mutation.
33013929	3	64	theme	database	719:726	arg1	up					728:729	GISAID database up	712:729	GISAID database up to April 26	712:741	We analyzed totally 7,813 sequences screened from 8,897 whole genome sequences on GISAID database up to April 26, and 18 S protein amino acid variations with relatively high frequency (≥10-3) were identified.
33013929	4	65	dep	variations	889:898	arg1	harboring					923:931	harboring	923:931	harboring the D614G mutation	923:950	A total of 228 sequences of variants had multiple variations, of note, most of them harboring the D614G mutation.
33013929	4	66	theme	variants	867:874	arg1	sequences					854:862	228 sequences	850:862	228 sequences of variants	850:874	A total of 228 sequences of variants had multiple variations, of note, most of them harboring the D614G mutation.
33013929	3	67	with	variations	772:781	arg1	≥10-3					815:819	≥10-3	815:819	≥10-3	815:819	We analyzed totally 7,813 sequences screened from 8,897 whole genome sequences on GISAID database up to April 26, and 18 S protein amino acid variations with relatively high frequency (≥10-3) were identified.
33013929	3	67	with	variations	772:781	arg1	frequency					804:812	relatively high frequency	788:812	relatively high frequency (≥10-3)	788:820	We analyzed totally 7,813 sequences screened from 8,897 whole genome sequences on GISAID database up to April 26, and 18 S protein amino acid variations with relatively high frequency (≥10-3) were identified.
33013929	2	68	theme	global	524:529	arg1	course					544:549	the global transmission course	520:549	the global transmission course	520:549	For better understanding the viral spike (S) protein variations and its potential effects on the interaction with the host immune system and also in vaccine development, the cell epitopes, glycosylation profile and their changes during the global transmission course were characterized and compared with SARS-CoV for their glycosylation profile.
33013929	5	69	theme	sites	1168:1172	arg1	structure					1123:1131	protein structure	1115:1131	protein structure	1115:1131	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	5	69	theme	sites	1168:1172	arg1	function					1151:1158	its potential function	1137:1158	its potential function	1137:1158	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	7	70	theme	important	1473:1481	arg1	snapshot					1483:1490	an important snapshot	1470:1490	an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates	1470:1670	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	3	71	theme	amino	761:765	arg1	variations					772:781	18 S protein amino acid variations	748:781	18 S protein amino acid variations with relatively high frequency (≥10-3)	748:820	We analyzed totally 7,813 sequences screened from 8,897 whole genome sequences on GISAID database up to April 26, and 18 S protein amino acid variations with relatively high frequency (≥10-3) were identified.
33013929	5	72	theme	B	983:983	arg1	epitopes					990:997	69 linear B cell epitopes	973:997	69 linear B cell epitopes	973:997	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	5	73	theme	potential	1141:1149	arg1	function					1151:1158	its potential function	1137:1158	its potential function	1137:1158	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	3	74	theme	S	751:751	arg1	variations					772:781	18 S protein amino acid variations	748:781	18 S protein amino acid variations with relatively high frequency (≥10-3)	748:820	We analyzed totally 7,813 sequences screened from 8,897 whole genome sequences on GISAID database up to April 26, and 18 S protein amino acid variations with relatively high frequency (≥10-3) were identified.
33013929	0	75	theme	Global	87:92	arg1	Course					107:112	Global Transmission Course	87:112	Global Transmission Course of COVID-19	87:124	Variations in SARS-CoV-2 Spike Protein Cell Epitopes and Glycosylation Profiles During Global Transmission Course of COVID-19.
33013929	7	76	theme	essential	1616:1624	arg1	epitopes					1564:1571	SARS-CoV-2 S protein cell epitopes	1538:1571	SARS-CoV-2 S protein cell epitopes	1538:1571	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	7	76	theme	essential	1616:1624	arg1	sites					1591:1595	glycosylation sites	1577:1595	glycosylation sites	1577:1595	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	7	76	theme	essential	1616:1624	arg1	basis					1626:1630	an essential basis	1613:1630	an essential basis for the selection of vaccine candidates	1613:1670	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	2	77	theme	vaccine	433:439	arg1	development					441:451	vaccine development	433:451	vaccine development	433:451	For better understanding the viral spike (S) protein variations and its potential effects on the interaction with the host immune system and also in vaccine development, the cell epitopes, glycosylation profile and their changes during the global transmission course were characterized and compared with SARS-CoV for their glycosylation profile.
33013929	5	78	theme	T	1051:1051	arg1	lymphocyte					1053:1062	41 cytotoxic T lymphocyte	1038:1062	41 cytotoxic T lymphocyte epitopes	1038:1071	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	4	79	theme	multiple	880:887	arg1	variations					889:898	multiple variations	880:898	multiple variations	880:898	A total of 228 sequences of variants had multiple variations, of note, most of them harboring the D614G mutation.
33013929	2	80	theme	glycosylation	607:619	arg1	profile					621:627	their glycosylation profile	601:627	their glycosylation profile	601:627	For better understanding the viral spike (S) protein variations and its potential effects on the interaction with the host immune system and also in vaccine development, the cell epitopes, glycosylation profile and their changes during the global transmission course were characterized and compared with SARS-CoV for their glycosylation profile.
33013929	0	81	theme	COVID-19	117:124	arg1	Course					107:112	Global Transmission Course	87:112	Global Transmission Course of COVID-19	87:124	Variations in SARS-CoV-2 Spike Protein Cell Epitopes and Glycosylation Profiles During Global Transmission Course of COVID-19.
33013929	5	82	theme	B	1018:1018	arg1	epitopes					1025:1032	175 discontinuous B cell epitopes	1000:1032	175 discontinuous B cell epitopes	1000:1032	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	2	83	theme	cell	458:461	arg1	epitopes					463:470	the cell epitopes	454:470	the cell epitopes	454:470	For better understanding the viral spike (S) protein variations and its potential effects on the interaction with the host immune system and also in vaccine development, the cell epitopes, glycosylation profile and their changes during the global transmission course were characterized and compared with SARS-CoV for their glycosylation profile.
33013929	2	84	theme	host	402:405	arg1	system					414:419	the host immune system	398:419	the host immune system	398:419	For better understanding the viral spike (S) protein variations and its potential effects on the interaction with the host immune system and also in vaccine development, the cell epitopes, glycosylation profile and their changes during the global transmission course were characterized and compared with SARS-CoV for their glycosylation profile.
33013929	0	85	theme	Spike	25:29	arg1	Epitopes					44:51	SARS-CoV-2 Spike Protein Cell Epitopes	14:51	SARS-CoV-2 Spike Protein Cell Epitopes	14:51	Variations in SARS-CoV-2 Spike Protein Cell Epitopes and Glycosylation Profiles During Global Transmission Course of COVID-19.
33013929	5	86	theme	S	1086:1086	arg1	protein					1088:1094	the viral S protein	1076:1094	the viral S protein	1076:1094	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	6	87	gly	N-glycosylation	1312:1326	arg2	sites					1328:1332	predicted N-glycosylation sites	1302:1332	predicted N-glycosylation sites	1302:1332	In addition, we detected 9 predicted N-glycosylation sites and 3 O-glycosylation sites unique to SARS-CoV-2, but no evidently observed variation of the glycan sites so far.
33013929	6	87	gly	N-glycosylation	1312:1326	arg2	9					1300:1300	9	1300:1300	9	1300:1300	In addition, we detected 9 predicted N-glycosylation sites and 3 O-glycosylation sites unique to SARS-CoV-2, but no evidently observed variation of the glycan sites so far.
33013929	4	88	contain	had	876:878	arg1	total					841:845	A total	839:845	A total of 228 sequences of variants	839:874	A total of 228 sequences of variants had multiple variations, of note, most of them harboring the D614G mutation.
33013929	4	88	contain	had	876:878	arg2	variations					889:898	multiple variations	880:898	multiple variations	880:898	A total of 228 sequences of variants had multiple variations, of note, most of them harboring the D614G mutation.
33013929	5	89	theme	linear	1195:1200	arg1	length					1210:1215	the linear epitope length	1191:1215	the linear epitope length	1191:1215	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	6	90	theme	predicted	1302:1310	arg1	sites					1328:1332	predicted N-glycosylation sites	1302:1332	predicted N-glycosylation sites	1302:1332	In addition, we detected 9 predicted N-glycosylation sites and 3 O-glycosylation sites unique to SARS-CoV-2, but no evidently observed variation of the glycan sites so far.
33013929	1	91	theme	pandemic	264:271	arg1	worldwide					273:281	a pandemic worldwide	262:281	a pandemic worldwide	262:281	Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to an outbreak of a pandemic worldwide.
33013929	7	92	theme	cell	1559:1562	arg1	epitopes					1564:1571	SARS-CoV-2 S protein cell epitopes	1538:1571	SARS-CoV-2 S protein cell epitopes	1538:1571	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	7	92	theme	cell	1559:1562	arg1	sites					1591:1595	glycosylation sites	1577:1595	glycosylation sites	1577:1595	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	7	92	theme	cell	1559:1562	arg1	basis					1626:1630	an essential basis	1613:1630	an essential basis for the selection of vaccine candidates	1613:1670	Our findings provided an important snapshot of temporal and geographical distributions on SARS-CoV-2 S protein cell epitopes and glycosylation sites, which would be an essential basis for the selection of vaccine candidates.
33013929	2	93	from	variations	337:346	arg1	interaction					381:391	the interaction	377:391	the interaction with the host immune system	377:419	For better understanding the viral spike (S) protein variations and its potential effects on the interaction with the host immune system and also in vaccine development, the cell epitopes, glycosylation profile and their changes during the global transmission course were characterized and compared with SARS-CoV for their glycosylation profile.
33013929	2	94	theme	viral	313:317	arg1	protein					329:335	the viral spike (S) protein	309:335	the viral spike (S) protein variations	309:346	For better understanding the viral spike (S) protein variations and its potential effects on the interaction with the host immune system and also in vaccine development, the cell epitopes, glycosylation profile and their changes during the global transmission course were characterized and compared with SARS-CoV for their glycosylation profile.
33013929	5	95	dep	shortened	1217:1225	arg1	such					1183:1186	such	1183:1186	such	1183:1186	Among the predicted 69 linear B cell epitopes, 175 discontinuous B cell epitopes and 41 cytotoxic T lymphocyte epitopes in the viral S protein, we found that the protein structure and its potential function of some sites changed, such as the linear epitope length shortened and discontinuous epitope disappeared of G476S.
33013929	2	96	from	effects	366:372	arg1	interaction					381:391	the interaction	377:391	the interaction with the host immune system	377:419	For better understanding the viral spike (S) protein variations and its potential effects on the interaction with the host immune system and also in vaccine development, the cell epitopes, glycosylation profile and their changes during the global transmission course were characterized and compared with SARS-CoV for their glycosylation profile.
34865748	7	0	theme	transcriptome	1224:1236	arg1	analysis					1238:1245	Colonic transcriptome analysis	1216:1245	Colonic transcriptome analysis	1216:1245	Colonic transcriptome analysis indicated the hypoglycemic effect of CSP was associated with the activation of the IGF1/PI3K/AKT signaling pathway.
34865748	3	1	theme	Coix	487:490	arg1	CSP					514:516	CSP	514:516	CSP	514:516	Since most plant polysaccharides are difficult to digest and absorb, we hypothesized that Coix seed polysaccharides (CSP) exert hypoglycemic effects through the gut.
34865748	3	1	theme	Coix	487:490	arg1	polysaccharides					497:511	Coix seed polysaccharides	487:511	Coix seed polysaccharides (CSP)	487:517	Since most plant polysaccharides are difficult to digest and absorb, we hypothesized that Coix seed polysaccharides (CSP) exert hypoglycemic effects through the gut.
34865748	2	2	with	patients	362:369	arg1	disorders					386:394	metabolic disorders	376:394	metabolic disorders	376:394	Coix seed (CS) as a homologous substance of traditional Chinese medicine and food, its polysaccharides can improve the symptoms of patients with metabolic disorders.
34865748	11	3	theme	microbial	1687:1695	arg1	composition					1697:1707	gut microbial composition	1683:1707	gut microbial composition	1683:1707	Our findings demonstrated that CSP treatment modulates gut microbial composition, especially of the SCFAs-producing bacteria, activates the IGF1/PI3K/AKT signaling pathways, and exhibits hypoglycemic efficacy.
34865748	2	4	theme	homologous	251:260	arg1	substance					262:270	a homologous substance	249:270	a homologous substance of traditional Chinese medicine and food	249:311	Coix seed (CS) as a homologous substance of traditional Chinese medicine and food, its polysaccharides can improve the symptoms of patients with metabolic disorders.
34865748	4	5	theme	CSP	639:641	arg1	effects					628:634	hypoglycemic effects	615:634	hypoglycemic effects of CSP on a T2DM mouse model	615:663	In this study, the underlying mechanisms regulating hypoglycemic effects of CSP on a T2DM mouse model were investigated.
34865748	5	6	theme	cholesterol	855:865	arg1	levels					867:872	total cholesterol, triglycerides and low-density lipoprotein cholesterol levels	794:872	total cholesterol, triglycerides and low-density lipoprotein cholesterol levels	794:872	After treatment with CSP, serum insulin and high-density lipoprotein cholesterol levels were increased, while total cholesterol, triglycerides and low-density lipoprotein cholesterol levels were decreased in T2DM mice.
34865748	2	7	theme	Chinese	287:293	arg1	medicine					295:302	traditional Chinese medicine	275:302	traditional Chinese medicine	275:302	Coix seed (CS) as a homologous substance of traditional Chinese medicine and food, its polysaccharides can improve the symptoms of patients with metabolic disorders.
34865748	4	8	from	effects	628:634	arg1	model					659:663	a T2DM mouse model	646:663	a T2DM mouse model	646:663	In this study, the underlying mechanisms regulating hypoglycemic effects of CSP on a T2DM mouse model were investigated.
34865748	0	9	theme	IGF1/PI3K/AKT	126:138	arg1	signaling					140:148	IGF1/PI3K/AKT signaling	126:148	IGF1/PI3K/AKT signaling	126:148	Coix seed polysaccharides alleviate type 2 diabetes mellitus via gut microbiota-derived short-chain fatty acids activation of IGF1/PI3K/AKT signaling.
34865748	5	10	theme	triglycerides	813:825	arg1	levels					867:872	total cholesterol, triglycerides and low-density lipoprotein cholesterol levels	794:872	total cholesterol, triglycerides and low-density lipoprotein cholesterol levels	794:872	After treatment with CSP, serum insulin and high-density lipoprotein cholesterol levels were increased, while total cholesterol, triglycerides and low-density lipoprotein cholesterol levels were decreased in T2DM mice.
34865748	8	11	theme	bacteria	1462:1469	arg1	increase					1434:1441	the increase	1430:1441	the increase of SCFAs-producing bacteria by CSP	1430:1476	Correlative analysis revealed that this activation may result from the increase of SCFAs-producing bacteria by CSP.
34865748	1	12	from	concern	206:212	arg1	years					224:228	recent years	217:228	recent years	217:228	Type 2 diabetes mellitus (T2DM) has become a worldwide concern in recent years.
34865748	5	13	theme	low-density	831:841	arg1	lipoprotein					843:853	low-density lipoprotein	831:853	low-density lipoprotein cholesterol	831:865	After treatment with CSP, serum insulin and high-density lipoprotein cholesterol levels were increased, while total cholesterol, triglycerides and low-density lipoprotein cholesterol levels were decreased in T2DM mice.
34865748	6	14	dep	helped	930:935	arg1	repair					937:942	repair	937:942	repair	937:942	In addition, CSP treatment helped repair the intestinal barrier and modulated the gut microbial composition in T2DM mice, mainly facilitating the growth of short-chain fatty acid (SCFA)-producing bacteria, Spearman's analysis revealed these bacteria were positively related with the hypoglycemic efficacy of CSP.
34865748	6	15	theme	CSP	1211:1213	arg1	efficacy					1199:1206	the hypoglycemic efficacy	1182:1206	the hypoglycemic efficacy of CSP	1182:1213	In addition, CSP treatment helped repair the intestinal barrier and modulated the gut microbial composition in T2DM mice, mainly facilitating the growth of short-chain fatty acid (SCFA)-producing bacteria, Spearman's analysis revealed these bacteria were positively related with the hypoglycemic efficacy of CSP.
34865748	6	16	theme	CSP	916:918	arg1	treatment					920:928	CSP treatment	916:928	CSP treatment	916:928	In addition, CSP treatment helped repair the intestinal barrier and modulated the gut microbial composition in T2DM mice, mainly facilitating the growth of short-chain fatty acid (SCFA)-producing bacteria, Spearman's analysis revealed these bacteria were positively related with the hypoglycemic efficacy of CSP.
34865748	11	17	theme	signaling	1782:1790	arg1	pathways					1792:1799	the IGF1/PI3K/AKT signaling pathways	1764:1799	the IGF1/PI3K/AKT signaling pathways	1764:1799	Our findings demonstrated that CSP treatment modulates gut microbial composition, especially of the SCFAs-producing bacteria, activates the IGF1/PI3K/AKT signaling pathways, and exhibits hypoglycemic efficacy.
34865748	9	18	theme	fecal	1533:1537	arg1	levels					1545:1550	fecal SCFAs levels	1533:1550	fecal SCFAs levels	1533:1550	GC-MS detection verified that CSP treatment increased fecal SCFAs levels.
34865748	6	19	theme	T2DM	1014:1017	arg1	mice					1019:1022	T2DM mice	1014:1022	T2DM mice	1014:1022	In addition, CSP treatment helped repair the intestinal barrier and modulated the gut microbial composition in T2DM mice, mainly facilitating the growth of short-chain fatty acid (SCFA)-producing bacteria, Spearman's analysis revealed these bacteria were positively related with the hypoglycemic efficacy of CSP.
34865748	7	20	theme	signaling	1344:1352	arg1	pathway					1354:1360	the IGF1/PI3K/AKT signaling pathway	1326:1360	the IGF1/PI3K/AKT signaling pathway	1326:1360	Colonic transcriptome analysis indicated the hypoglycemic effect of CSP was associated with the activation of the IGF1/PI3K/AKT signaling pathway.
34865748	4	21	theme	mouse	653:657	arg1	model					659:663	a T2DM mouse model	646:663	a T2DM mouse model	646:663	In this study, the underlying mechanisms regulating hypoglycemic effects of CSP on a T2DM mouse model were investigated.
34865748	7	22	theme	Colonic	1216:1222	arg1	analysis					1238:1245	Colonic transcriptome analysis	1216:1245	Colonic transcriptome analysis	1216:1245	Colonic transcriptome analysis indicated the hypoglycemic effect of CSP was associated with the activation of the IGF1/PI3K/AKT signaling pathway.
34865748	1	23	theme	diabetes	158:165	arg1	T2DM					177:180	T2DM	177:180	T2DM	177:180	Type 2 diabetes mellitus (T2DM) has become a worldwide concern in recent years.
34865748	1	23	theme	diabetes	158:165	arg1	mellitus					167:174	Type 2 diabetes mellitus	151:174	Type 2 diabetes mellitus (T2DM)	151:181	Type 2 diabetes mellitus (T2DM) has become a worldwide concern in recent years.
34865748	0	24	theme	microbiota-derived	69:86	arg1	acids					106:110	gut microbiota-derived short-chain fatty acids	65:110	gut microbiota-derived short-chain fatty acids activation of IGF1/PI3K/AKT signaling	65:148	Coix seed polysaccharides alleviate type 2 diabetes mellitus via gut microbiota-derived short-chain fatty acids activation of IGF1/PI3K/AKT signaling.
34865748	6	25	theme	gut	985:987	arg1	composition					999:1009	the gut microbial composition	981:1009	the gut microbial composition	981:1009	In addition, CSP treatment helped repair the intestinal barrier and modulated the gut microbial composition in T2DM mice, mainly facilitating the growth of short-chain fatty acid (SCFA)-producing bacteria, Spearman's analysis revealed these bacteria were positively related with the hypoglycemic efficacy of CSP.
34865748	0	26	theme	fatty	100:104	arg1	acids					106:110	gut microbiota-derived short-chain fatty acids	65:110	gut microbiota-derived short-chain fatty acids activation of IGF1/PI3K/AKT signaling	65:148	Coix seed polysaccharides alleviate type 2 diabetes mellitus via gut microbiota-derived short-chain fatty acids activation of IGF1/PI3K/AKT signaling.
34865748	1	27	theme	Type	151:154	arg1	T2DM					177:180	T2DM	177:180	T2DM	177:180	Type 2 diabetes mellitus (T2DM) has become a worldwide concern in recent years.
34865748	1	27	theme	Type	151:154	arg1	mellitus					167:174	Type 2 diabetes mellitus	151:174	Type 2 diabetes mellitus (T2DM)	151:181	Type 2 diabetes mellitus (T2DM) has become a worldwide concern in recent years.
34865748	8	28	theme	Correlative	1363:1373	arg1	analysis					1375:1382	Correlative analysis	1363:1382	Correlative analysis	1363:1382	Correlative analysis revealed that this activation may result from the increase of SCFAs-producing bacteria by CSP.
34865748	5	29	theme	serum	710:714	arg1	insulin					716:722	serum insulin	710:722	serum insulin	710:722	After treatment with CSP, serum insulin and high-density lipoprotein cholesterol levels were increased, while total cholesterol, triglycerides and low-density lipoprotein cholesterol levels were decreased in T2DM mice.
34865748	6	30	theme	short-chain	1059:1069	arg1	SCFA					1083:1086	SCFA	1083:1086	SCFA	1083:1086	In addition, CSP treatment helped repair the intestinal barrier and modulated the gut microbial composition in T2DM mice, mainly facilitating the growth of short-chain fatty acid (SCFA)-producing bacteria, Spearman's analysis revealed these bacteria were positively related with the hypoglycemic efficacy of CSP.
34865748	6	30	theme	short-chain	1059:1069	arg1	acid					1077:1080	short-chain fatty acid	1059:1080	short-chain fatty acid (SCFA)-producing bacteria	1059:1106	In addition, CSP treatment helped repair the intestinal barrier and modulated the gut microbial composition in T2DM mice, mainly facilitating the growth of short-chain fatty acid (SCFA)-producing bacteria, Spearman's analysis revealed these bacteria were positively related with the hypoglycemic efficacy of CSP.
34865748	0	31	theme	gut	65:67	arg1	acids					106:110	gut microbiota-derived short-chain fatty acids	65:110	gut microbiota-derived short-chain fatty acids activation of IGF1/PI3K/AKT signaling	65:148	Coix seed polysaccharides alleviate type 2 diabetes mellitus via gut microbiota-derived short-chain fatty acids activation of IGF1/PI3K/AKT signaling.
34865748	3	32	theme	most	403:406	arg1	polysaccharides					414:428	most plant polysaccharides	403:428	most plant polysaccharides	403:428	Since most plant polysaccharides are difficult to digest and absorb, we hypothesized that Coix seed polysaccharides (CSP) exert hypoglycemic effects through the gut.
34865748	0	33	theme	Coix	0:3	arg1	polysaccharides					10:24	Coix seed polysaccharides	0:24	Coix seed polysaccharides	0:24	Coix seed polysaccharides alleviate type 2 diabetes mellitus via gut microbiota-derived short-chain fatty acids activation of IGF1/PI3K/AKT signaling.
34865748	5	34	theme	cholesterol	800:810	arg1	levels					867:872	total cholesterol, triglycerides and low-density lipoprotein cholesterol levels	794:872	total cholesterol, triglycerides and low-density lipoprotein cholesterol levels	794:872	After treatment with CSP, serum insulin and high-density lipoprotein cholesterol levels were increased, while total cholesterol, triglycerides and low-density lipoprotein cholesterol levels were decreased in T2DM mice.
34865748	5	35	with	treatment	690:698	arg1	CSP					705:707	CSP	705:707	CSP	705:707	After treatment with CSP, serum insulin and high-density lipoprotein cholesterol levels were increased, while total cholesterol, triglycerides and low-density lipoprotein cholesterol levels were decreased in T2DM mice.
34865748	0	36	theme	type	36:39	arg1	mellitus					52:59	type 2 diabetes mellitus	36:59	type 2 diabetes mellitus	36:59	Coix seed polysaccharides alleviate type 2 diabetes mellitus via gut microbiota-derived short-chain fatty acids activation of IGF1/PI3K/AKT signaling.
34865748	10	37	theme	Molecular	1553:1561	arg1	docking					1563:1569	Molecular docking	1553:1569	Molecular docking	1553:1569	Molecular docking revealed that SCFAs could bind with IGF1, PI3K, and AKT.
34865748	0	38	theme	diabetes	43:50	arg1	mellitus					52:59	type 2 diabetes mellitus	36:59	type 2 diabetes mellitus	36:59	Coix seed polysaccharides alleviate type 2 diabetes mellitus via gut microbiota-derived short-chain fatty acids activation of IGF1/PI3K/AKT signaling.
34865748	7	39	theme	CSP	1284:1286	arg1	effect					1274:1279	the hypoglycemic effect	1257:1279	the hypoglycemic effect of CSP	1257:1286	Colonic transcriptome analysis indicated the hypoglycemic effect of CSP was associated with the activation of the IGF1/PI3K/AKT signaling pathway.
34865748	2	40	theme	food	308:311	arg1	substance					262:270	a homologous substance	249:270	a homologous substance of traditional Chinese medicine and food	249:311	Coix seed (CS) as a homologous substance of traditional Chinese medicine and food, its polysaccharides can improve the symptoms of patients with metabolic disorders.
34865748	1	41	theme	recent	217:222	arg1	years					224:228	recent years	217:228	recent years	217:228	Type 2 diabetes mellitus (T2DM) has become a worldwide concern in recent years.
34865748	11	42	theme	bacteria	1744:1751	arg1	composition					1697:1707	gut microbial composition	1683:1707	gut microbial composition	1683:1707	Our findings demonstrated that CSP treatment modulates gut microbial composition, especially of the SCFAs-producing bacteria, activates the IGF1/PI3K/AKT signaling pathways, and exhibits hypoglycemic efficacy.
34865748	8	43	theme	SCFAs-producing	1446:1460	arg1	bacteria					1462:1469	SCFAs-producing bacteria	1446:1469	SCFAs-producing bacteria	1446:1469	Correlative analysis revealed that this activation may result from the increase of SCFAs-producing bacteria by CSP.
34865748	3	44	theme	seed	492:495	arg1	CSP					514:516	CSP	514:516	CSP	514:516	Since most plant polysaccharides are difficult to digest and absorb, we hypothesized that Coix seed polysaccharides (CSP) exert hypoglycemic effects through the gut.
34865748	3	44	theme	seed	492:495	arg1	polysaccharides					497:511	Coix seed polysaccharides	487:511	Coix seed polysaccharides (CSP)	487:517	Since most plant polysaccharides are difficult to digest and absorb, we hypothesized that Coix seed polysaccharides (CSP) exert hypoglycemic effects through the gut.
34865748	5	45	theme	high-density	728:739	arg1	cholesterol					753:763	high-density lipoprotein cholesterol	728:763	high-density lipoprotein cholesterol	728:763	After treatment with CSP, serum insulin and high-density lipoprotein cholesterol levels were increased, while total cholesterol, triglycerides and low-density lipoprotein cholesterol levels were decreased in T2DM mice.
34865748	11	46	theme	gut	1683:1685	arg1	composition					1697:1707	gut microbial composition	1683:1707	gut microbial composition	1683:1707	Our findings demonstrated that CSP treatment modulates gut microbial composition, especially of the SCFAs-producing bacteria, activates the IGF1/PI3K/AKT signaling pathways, and exhibits hypoglycemic efficacy.
34865748	6	47	theme	acid	1077:1080	arg1	growth					1049:1054	the growth	1045:1054	the growth of short-chain fatty acid (SCFA)-producing bacteria	1045:1106	In addition, CSP treatment helped repair the intestinal barrier and modulated the gut microbial composition in T2DM mice, mainly facilitating the growth of short-chain fatty acid (SCFA)-producing bacteria, Spearman's analysis revealed these bacteria were positively related with the hypoglycemic efficacy of CSP.
34865748	5	48	theme	lipoprotein	843:853	arg1	cholesterol					855:865	low-density lipoprotein cholesterol	831:865	low-density lipoprotein cholesterol	831:865	After treatment with CSP, serum insulin and high-density lipoprotein cholesterol levels were increased, while total cholesterol, triglycerides and low-density lipoprotein cholesterol levels were decreased in T2DM mice.
34865748	2	49	dep	seed	236:239	arg1	substance					262:270	a homologous substance	249:270	a homologous substance of traditional Chinese medicine and food	249:311	Coix seed (CS) as a homologous substance of traditional Chinese medicine and food, its polysaccharides can improve the symptoms of patients with metabolic disorders.
34865748	5	50	theme	lipoprotein	741:751	arg1	cholesterol					753:763	high-density lipoprotein cholesterol	728:763	high-density lipoprotein cholesterol	728:763	After treatment with CSP, serum insulin and high-density lipoprotein cholesterol levels were increased, while total cholesterol, triglycerides and low-density lipoprotein cholesterol levels were decreased in T2DM mice.
34865748	2	51	theme	medicine	295:302	arg1	substance					262:270	a homologous substance	249:270	a homologous substance of traditional Chinese medicine and food	249:311	Coix seed (CS) as a homologous substance of traditional Chinese medicine and food, its polysaccharides can improve the symptoms of patients with metabolic disorders.
34865748	0	52	theme	signaling	140:148	arg1	activation					112:121	gut microbiota-derived short-chain fatty acids activation	65:121	gut microbiota-derived short-chain fatty acids activation of IGF1/PI3K/AKT signaling	65:148	Coix seed polysaccharides alleviate type 2 diabetes mellitus via gut microbiota-derived short-chain fatty acids activation of IGF1/PI3K/AKT signaling.
34865748	2	53	theme	traditional	275:285	arg1	medicine					295:302	traditional Chinese medicine	275:302	traditional Chinese medicine	275:302	Coix seed (CS) as a homologous substance of traditional Chinese medicine and food, its polysaccharides can improve the symptoms of patients with metabolic disorders.
34865748	9	54	theme	GC-MS	1479:1483	arg1	detection					1485:1493	GC-MS detection	1479:1493	GC-MS detection	1479:1493	GC-MS detection verified that CSP treatment increased fecal SCFAs levels.
34865748	6	55	with	related	1169:1175	arg1	efficacy					1199:1206	the hypoglycemic efficacy	1182:1206	the hypoglycemic efficacy of CSP	1182:1213	In addition, CSP treatment helped repair the intestinal barrier and modulated the gut microbial composition in T2DM mice, mainly facilitating the growth of short-chain fatty acid (SCFA)-producing bacteria, Spearman's analysis revealed these bacteria were positively related with the hypoglycemic efficacy of CSP.
34865748	4	56	theme	hypoglycemic	615:626	arg1	effects					628:634	hypoglycemic effects	615:634	hypoglycemic effects of CSP on a T2DM mouse model	615:663	In this study, the underlying mechanisms regulating hypoglycemic effects of CSP on a T2DM mouse model were investigated.
34865748	9	57	theme	CSP	1509:1511	arg1	treatment					1513:1521	CSP treatment	1509:1521	CSP treatment	1509:1521	GC-MS detection verified that CSP treatment increased fecal SCFAs levels.
34865748	3	58	theme	hypoglycemic	525:536	arg1	effects					538:544	hypoglycemic effects	525:544	hypoglycemic effects	525:544	Since most plant polysaccharides are difficult to digest and absorb, we hypothesized that Coix seed polysaccharides (CSP) exert hypoglycemic effects through the gut.
34865748	6	59	theme	hypoglycemic	1186:1197	arg1	efficacy					1199:1206	the hypoglycemic efficacy	1182:1206	the hypoglycemic efficacy of CSP	1182:1213	In addition, CSP treatment helped repair the intestinal barrier and modulated the gut microbial composition in T2DM mice, mainly facilitating the growth of short-chain fatty acid (SCFA)-producing bacteria, Spearman's analysis revealed these bacteria were positively related with the hypoglycemic efficacy of CSP.
34865748	5	60	theme	insulin	716:722	arg1	levels					765:770	serum insulin and high-density lipoprotein cholesterol levels	710:770	serum insulin and high-density lipoprotein cholesterol levels	710:770	After treatment with CSP, serum insulin and high-density lipoprotein cholesterol levels were increased, while total cholesterol, triglycerides and low-density lipoprotein cholesterol levels were decreased in T2DM mice.
34865748	9	61	theme	SCFAs	1539:1543	arg1	levels					1545:1550	fecal SCFAs levels	1533:1550	fecal SCFAs levels	1533:1550	GC-MS detection verified that CSP treatment increased fecal SCFAs levels.
34865748	7	62	theme	pathway	1354:1360	arg1	activation					1312:1321	the activation	1308:1321	the activation of the IGF1/PI3K/AKT signaling pathway	1308:1360	Colonic transcriptome analysis indicated the hypoglycemic effect of CSP was associated with the activation of the IGF1/PI3K/AKT signaling pathway.
34865748	4	63	theme	T2DM	648:651	arg1	model					659:663	a T2DM mouse model	646:663	a T2DM mouse model	646:663	In this study, the underlying mechanisms regulating hypoglycemic effects of CSP on a T2DM mouse model were investigated.
34865748	11	64	theme	IGF1/PI3K/AKT	1768:1780	arg1	pathways					1792:1799	the IGF1/PI3K/AKT signaling pathways	1764:1799	the IGF1/PI3K/AKT signaling pathways	1764:1799	Our findings demonstrated that CSP treatment modulates gut microbial composition, especially of the SCFAs-producing bacteria, activates the IGF1/PI3K/AKT signaling pathways, and exhibits hypoglycemic efficacy.
34865748	0	65	theme	short-chain	88:98	arg1	acids					106:110	gut microbiota-derived short-chain fatty acids	65:110	gut microbiota-derived short-chain fatty acids activation of IGF1/PI3K/AKT signaling	65:148	Coix seed polysaccharides alleviate type 2 diabetes mellitus via gut microbiota-derived short-chain fatty acids activation of IGF1/PI3K/AKT signaling.
34865748	6	66	theme	intestinal	948:957	arg1	barrier					959:965	the intestinal barrier	944:965	the intestinal barrier	944:965	In addition, CSP treatment helped repair the intestinal barrier and modulated the gut microbial composition in T2DM mice, mainly facilitating the growth of short-chain fatty acid (SCFA)-producing bacteria, Spearman's analysis revealed these bacteria were positively related with the hypoglycemic efficacy of CSP.
34865748	5	67	theme	T2DM	892:895	arg1	mice					897:900	T2DM mice	892:900	T2DM mice	892:900	After treatment with CSP, serum insulin and high-density lipoprotein cholesterol levels were increased, while total cholesterol, triglycerides and low-density lipoprotein cholesterol levels were decreased in T2DM mice.
34865748	6	68	theme	microbial	989:997	arg1	composition					999:1009	the gut microbial composition	981:1009	the gut microbial composition	981:1009	In addition, CSP treatment helped repair the intestinal barrier and modulated the gut microbial composition in T2DM mice, mainly facilitating the growth of short-chain fatty acid (SCFA)-producing bacteria, Spearman's analysis revealed these bacteria were positively related with the hypoglycemic efficacy of CSP.
34865748	11	69	theme	hypoglycemic	1815:1826	arg1	efficacy					1828:1835	hypoglycemic efficacy	1815:1835	hypoglycemic efficacy	1815:1835	Our findings demonstrated that CSP treatment modulates gut microbial composition, especially of the SCFAs-producing bacteria, activates the IGF1/PI3K/AKT signaling pathways, and exhibits hypoglycemic efficacy.
34865748	0	70	theme	acids	106:110	arg1	activation					112:121	gut microbiota-derived short-chain fatty acids activation	65:121	gut microbiota-derived short-chain fatty acids activation of IGF1/PI3K/AKT signaling	65:148	Coix seed polysaccharides alleviate type 2 diabetes mellitus via gut microbiota-derived short-chain fatty acids activation of IGF1/PI3K/AKT signaling.
34865748	1	71	theme	worldwide	196:204	arg1	concern					206:212	a worldwide concern	194:212	a worldwide concern in recent years	194:228	Type 2 diabetes mellitus (T2DM) has become a worldwide concern in recent years.
34865748	6	72	theme	fatty	1071:1075	arg1	SCFA					1083:1086	SCFA	1083:1086	SCFA	1083:1086	In addition, CSP treatment helped repair the intestinal barrier and modulated the gut microbial composition in T2DM mice, mainly facilitating the growth of short-chain fatty acid (SCFA)-producing bacteria, Spearman's analysis revealed these bacteria were positively related with the hypoglycemic efficacy of CSP.
34865748	6	72	theme	fatty	1071:1075	arg1	acid					1077:1080	short-chain fatty acid	1059:1080	short-chain fatty acid (SCFA)-producing bacteria	1059:1106	In addition, CSP treatment helped repair the intestinal barrier and modulated the gut microbial composition in T2DM mice, mainly facilitating the growth of short-chain fatty acid (SCFA)-producing bacteria, Spearman's analysis revealed these bacteria were positively related with the hypoglycemic efficacy of CSP.
34865748	6	73	dep	mice	1019:1022	arg1	facilitating					1032:1043	facilitating	1032:1043	facilitating the growth of short-chain fatty acid (SCFA)-producing bacteria	1032:1106	In addition, CSP treatment helped repair the intestinal barrier and modulated the gut microbial composition in T2DM mice, mainly facilitating the growth of short-chain fatty acid (SCFA)-producing bacteria, Spearman's analysis revealed these bacteria were positively related with the hypoglycemic efficacy of CSP.
34865748	2	74	theme	patients	362:369	arg1	symptoms					350:357	the symptoms	346:357	the symptoms of patients with metabolic disorders	346:394	Coix seed (CS) as a homologous substance of traditional Chinese medicine and food, its polysaccharides can improve the symptoms of patients with metabolic disorders.
34865748	0	75	theme	seed	5:8	arg1	polysaccharides					10:24	Coix seed polysaccharides	0:24	Coix seed polysaccharides	0:24	Coix seed polysaccharides alleviate type 2 diabetes mellitus via gut microbiota-derived short-chain fatty acids activation of IGF1/PI3K/AKT signaling.
34865748	3	76	theme	plant	408:412	arg1	polysaccharides					414:428	most plant polysaccharides	403:428	most plant polysaccharides	403:428	Since most plant polysaccharides are difficult to digest and absorb, we hypothesized that Coix seed polysaccharides (CSP) exert hypoglycemic effects through the gut.
34865748	5	77	theme	total	794:798	arg1	cholesterol					800:810	total cholesterol	794:810	total cholesterol	794:810	After treatment with CSP, serum insulin and high-density lipoprotein cholesterol levels were increased, while total cholesterol, triglycerides and low-density lipoprotein cholesterol levels were decreased in T2DM mice.
34865748	5	78	theme	cholesterol	753:763	arg1	levels					765:770	serum insulin and high-density lipoprotein cholesterol levels	710:770	serum insulin and high-density lipoprotein cholesterol levels	710:770	After treatment with CSP, serum insulin and high-density lipoprotein cholesterol levels were increased, while total cholesterol, triglycerides and low-density lipoprotein cholesterol levels were decreased in T2DM mice.
34865748	2	79	theme	metabolic	376:384	arg1	disorders					386:394	metabolic disorders	376:394	metabolic disorders	376:394	Coix seed (CS) as a homologous substance of traditional Chinese medicine and food, its polysaccharides can improve the symptoms of patients with metabolic disorders.
34865748	0	80	link	microbiota-derived	69:86	arg1	acids					106:110	gut microbiota-derived short-chain fatty acids	65:110	gut microbiota-derived short-chain fatty acids activation of IGF1/PI3K/AKT signaling	65:148	Coix seed polysaccharides alleviate type 2 diabetes mellitus via gut microbiota-derived short-chain fatty acids activation of IGF1/PI3K/AKT signaling.
34865748	7	81	theme	hypoglycemic	1261:1272	arg1	effect					1274:1279	the hypoglycemic effect	1257:1279	the hypoglycemic effect of CSP	1257:1286	Colonic transcriptome analysis indicated the hypoglycemic effect of CSP was associated with the activation of the IGF1/PI3K/AKT signaling pathway.
34865748	11	82	theme	SCFAs-producing	1728:1742	arg1	bacteria					1744:1751	the SCFAs-producing bacteria	1724:1751	the SCFAs-producing bacteria	1724:1751	Our findings demonstrated that CSP treatment modulates gut microbial composition, especially of the SCFAs-producing bacteria, activates the IGF1/PI3K/AKT signaling pathways, and exhibits hypoglycemic efficacy.
34865748	7	83	theme	IGF1/PI3K/AKT	1330:1342	arg1	pathway					1354:1360	the IGF1/PI3K/AKT signaling pathway	1326:1360	the IGF1/PI3K/AKT signaling pathway	1326:1360	Colonic transcriptome analysis indicated the hypoglycemic effect of CSP was associated with the activation of the IGF1/PI3K/AKT signaling pathway.
34865748	4	84	theme	underlying	582:591	arg1	mechanisms					593:602	the underlying mechanisms	578:602	the underlying mechanisms regulating hypoglycemic effects of CSP on a T2DM mouse model	578:663	In this study, the underlying mechanisms regulating hypoglycemic effects of CSP on a T2DM mouse model were investigated.
34865748	11	85	theme	CSP	1659:1661	arg1	treatment					1663:1671	CSP treatment	1659:1671	CSP treatment	1659:1671	Our findings demonstrated that CSP treatment modulates gut microbial composition, especially of the SCFAs-producing bacteria, activates the IGF1/PI3K/AKT signaling pathways, and exhibits hypoglycemic efficacy.
34731210	14	0	theme	auxin	1395:1399	arg1	products					1424:1431	auxin biosynthesis subsystem products	1395:1431	auxin biosynthesis subsystem products	1395:1431	Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified.
34731210	17	1	theme	Curtobacterium	1627:1640	arg1	sp					1642:1643	Curtobacterium sp	1627:1643	Curtobacterium sp	1627:1643	Therefore, Curtobacterium sp.
34731210	16	2	theme	polysaccharides	1599:1613	arg1	hydrolysis					1575:1584	the hydrolysis	1571:1584	the hydrolysis of different polysaccharides	1571:1613	GD1 suggests that it has the tools for the hydrolysis of different polysaccharides.
34731210	14	3	theme	carbohydrates	1201:1213	arg1	catabolism					1176:1185	catabolism	1176:1185	catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products	1176:1431	Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified.
34731210	15	4	theme	glycosyl	1476:1483	arg1	hydrolases					1485:1494	putative glycosyl hydrolases	1467:1494	putative glycosyl hydrolases	1467:1494	The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp.
34731210	13	5	theme	chitinase	1040:1048	arg1	production					1050:1059	chitinase production	1040:1059	chitinase production	1040:1059	The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed.
34731210	5	6	theme	chitinase	342:350	arg1	activity					352:359	a strong chitinase activity	333:359	a strong chitinase activity	333:359	GD1 showed a strong chitinase activity.
34731210	13	7	theme	genes	1128:1132	arg1	pattern					1097:1103	the distribution pattern	1080:1103	the distribution pattern of glycosyl hydrolases genes	1080:1132	The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed.
34731210	7	8	dep	kDa	562:564	arg1	U/mg					593:596	90.8 U/mg	588:596	90.8 U/mg	588:596	The chitinase was partially purified and characterized: molecular weight was approximately 37 kDa and specific activity 90.8 U/mg.
34731210	10	9	theme	Curtobacterium	728:741	arg1	spp					743:745	four other Curtobacterium spp	717:745	four other Curtobacterium spp	717:745	Our isolate formed a phylogenetic cluster with four other Curtobacterium spp.
34731210	16	10	theme	different	1589:1597	arg1	polysaccharides					1599:1613	different polysaccharides	1589:1613	different polysaccharides	1589:1613	GD1 suggests that it has the tools for the hydrolysis of different polysaccharides.
34731210	12	11	theme	sequences	902:910	arg1	comparison					881:890	comparison	881:890	comparison of genome sequences of strains forming new cluster	881:941	The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved.
34731210	12	11	theme	sequences	902:910	arg1	visualization					863:875	The circular genome visualization	843:875	The circular genome visualization	843:875	The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved.
34731210	14	12	theme	structural	1190:1199	arg1	products					1424:1431	auxin biosynthesis subsystem products	1395:1431	auxin biosynthesis subsystem products	1395:1431	Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified.
34731210	14	12	theme	structural	1190:1199	arg1	compounds					1381:1389	toxic compounds	1375:1389	toxic compounds	1375:1389	Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified.
34731210	14	12	theme	structural	1190:1199	arg1	oligosaccharides					1223:1238	oligosaccharides	1223:1238	oligosaccharides	1223:1238	Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified.
34731210	14	12	theme	structural	1190:1199	arg1	carbohydrates					1201:1213	structural carbohydrates	1190:1213	structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products	1190:1431	Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified.
34731210	14	12	theme	structural	1190:1199	arg1	genes					1309:1313	genes	1309:1313	genes	1309:1313	Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified.
34731210	14	12	theme	structural	1190:1199	arg1	polysaccharides					1281:1295	plant and animal polysaccharides	1264:1295	polysaccharides	1281:1295	Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified.
34731210	14	12	theme	structural	1190:1199	arg1	polysaccharides					1247:1261	mixed polysaccharides	1241:1261	mixed polysaccharides	1241:1261	Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified.
34731210	11	13	with	strains	748:754	arg1	ANIb/ANIm					762:770	ANIb/ANIm	762:770	ANIb/ANIm	762:770	strains, with ANIb/ANIm ≥ 98%, representing a new, still non described Curtobacterium species.
34731210	10	14	theme	other	722:726	arg1	spp					743:745	four other Curtobacterium spp	717:745	four other Curtobacterium spp	717:745	Our isolate formed a phylogenetic cluster with four other Curtobacterium spp.
34731210	12	15	theme	most	958:961	arg1	regions					963:969	most regions	958:969	most regions within their genomes	958:990	The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved.
34731210	10	16	with	cluster	704:710	arg1	spp					743:745	four other Curtobacterium spp	717:745	four other Curtobacterium spp	717:745	Our isolate formed a phylogenetic cluster with four other Curtobacterium spp.
34731210	4	17	theme	same	285:288	arg1	origin					290:295	the same origin	281:295	the same origin	281:295	It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp.
34731210	15	18	theme	hydrolases	1485:1494	arg1	abundance					1454:1462	The abundance	1450:1462	The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp.	1450:1530	The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp.
34731210	12	19	theme	strains	915:921	arg1	sequences					902:910	genome sequences	895:910	genome sequences of strains forming new cluster	895:941	The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved.
34731210	1	20	theme	field-grown	66:76	arg1	soybean					78:84	field-grown soybean and analysis	66:97	soybean	78:84	isolated from field-grown soybean and analysis of its genome sequence.
34731210	4	21	dep	sp	318:319	arg1	bacteria					247:254	all bacteria	243:254	all bacteria previously isolated from the same origin	243:295	It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp.
34731210	18	22	theme	plant	1735:1739	arg1	responses					1749:1757	the plant defense responses	1731:1757	the plant defense responses	1731:1757	GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader.
34731210	11	23	theme	new	794:796	arg1	species					834:840	a new, still non described Curtobacterium species	792:840	a new, still non described Curtobacterium species	792:840	strains, with ANIb/ANIm ≥ 98%, representing a new, still non described Curtobacterium species.
34731210	14	24	theme	animal	1274:1279	arg1	polysaccharides					1281:1295	plant and animal polysaccharides	1264:1295	polysaccharides	1281:1295	Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified.
34731210	7	25	theme	specific	570:577	arg1	activity					579:586	specific activity	570:586	specific activity	570:586	The chitinase was partially purified and characterized: molecular weight was approximately 37 kDa and specific activity 90.8 U/mg.
34731210	14	26	theme	biosynthesis	1401:1412	arg1	products					1424:1431	auxin biosynthesis subsystem products	1395:1431	auxin biosynthesis subsystem products	1395:1431	Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified.
34731210	0	27	theme	chitinolytic	4:15	arg1	activity					17:24	The chitinolytic activity	0:24	The chitinolytic activity of the Curtobacterium sp	0:49	The chitinolytic activity of the Curtobacterium sp.
34731210	18	28	theme	biocontrol	1698:1707	arg1	agent					1709:1713	biocontrol agent	1698:1713	biocontrol agent	1698:1713	GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader.
34731210	18	29	attach	isolated	1650:1657	arg2	bioremediator					1683:1695	a bioremediator	1681:1695	a bioremediator	1681:1695	GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader.
34731210	18	29	attach	isolated	1650:1657	arg2	GD1					1646:1648	GD1	1646:1648	GD1 isolated from soybean	1646:1670	GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader.
34731210	18	29	attach	isolated	1650:1657	arg1	soybean					1664:1670	soybean	1664:1670	soybean	1664:1670	GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader.
34731210	14	30	theme	plant	1264:1268	arg1	polysaccharides					1281:1295	plant and animal polysaccharides	1264:1295	polysaccharides	1281:1295	Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified.
34731210	12	31	theme	genome	895:900	arg1	sequences					902:910	genome sequences	895:910	genome sequences of strains forming new cluster	895:941	The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved.
34731210	6	32	attach	presence	424:431	arg1	medium					460:465	the medium	456:465	the medium	456:465	The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium.
34731210	6	32	attach	presence	424:431	arg2	chitin					446:451	colloidal chitin	436:451	colloidal chitin	436:451	The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium.
34731210	13	33	theme	glycosyl	1108:1115	arg1	genes					1128:1132	glycosyl hydrolases genes	1108:1132	glycosyl hydrolases genes	1108:1132	The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed.
34731210	3	34	theme	grown	189:193	arg1	soybean					195:201	conventionally grown soybean	174:201	conventionally grown soybean in Brazil	174:211	GD1 was isolated from leaves of conventionally grown soybean in Brazil.
34731210	15	35	theme	putative	1467:1474	arg1	hydrolases					1485:1494	putative glycosyl hydrolases	1467:1494	putative glycosyl hydrolases	1467:1494	The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp.
34731210	12	36	theme	new	931:933	arg1	cluster					935:941	new cluster	931:941	new cluster	931:941	The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved.
34731210	0	37	theme	Curtobacterium	33:46	arg1	sp					48:49	the Curtobacterium sp	29:49	the Curtobacterium sp	29:49	The chitinolytic activity of the Curtobacterium sp.
34731210	5	38	theme	strong	335:340	arg1	activity					352:359	a strong chitinase activity	333:359	a strong chitinase activity	333:359	GD1 showed a strong chitinase activity.
34731210	14	39	theme	mixed	1241:1245	arg1	polysaccharides					1247:1261	mixed polysaccharides	1241:1261	mixed polysaccharides	1241:1261	Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified.
34731210	7	40	theme	molecular	524:532	arg1	weight					534:539	molecular weight	524:539	molecular weight	524:539	The chitinase was partially purified and characterized: molecular weight was approximately 37 kDa and specific activity 90.8 U/mg.
34731210	13	41	theme	distribution	1084:1095	arg1	pattern					1097:1103	the distribution pattern	1080:1103	the distribution pattern of glycosyl hydrolases genes	1080:1132	The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed.
34731210	14	42	theme	gene	1318:1321	arg1	clusters					1323:1330	gene clusters	1318:1330	gene clusters	1318:1330	Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified.
34731210	11	43	dep	new	794:796	arg1	non					805:807	non	805:807	non	805:807	strains, with ANIb/ANIm ≥ 98%, representing a new, still non described Curtobacterium species.
34731210	18	44	theme	responses	1749:1757	arg1	bioremediator					1683:1695	a bioremediator	1681:1695	a bioremediator	1681:1695	GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader.
34731210	18	44	theme	responses	1749:1757	arg1	agent					1709:1713	biocontrol agent	1698:1713	biocontrol agent	1698:1713	GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader.
34731210	18	44	theme	responses	1749:1757	arg1	elicitor					1719:1726	an elicitor	1716:1726	an elicitor of the plant defense responses	1716:1757	GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader.
34731210	18	44	theme	responses	1749:1757	arg1	GD1					1646:1648	GD1	1646:1648	GD1 isolated from soybean	1646:1670	GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader.
34731210	18	44	theme	responses	1749:1757	arg1	degrader					1769:1776	degrader	1769:1776	degrader	1769:1776	GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader.
34731210	11	45	theme	described	809:817	arg1	species					834:840	a new, still non described Curtobacterium species	792:840	a new, still non described Curtobacterium species	792:840	strains, with ANIb/ANIm ≥ 98%, representing a new, still non described Curtobacterium species.
34731210	3	46	attach	isolated	150:157	arg1	leaves					164:169	leaves	164:169	leaves	164:169	GD1 was isolated from leaves of conventionally grown soybean in Brazil.
34731210	3	46	attach	isolated	150:157	arg2	GD1					142:144	GD1	142:144	GD1	142:144	GD1 was isolated from leaves of conventionally grown soybean in Brazil.
34731210	18	47	theme	defense	1741:1747	arg1	responses					1749:1757	the plant defense responses	1731:1757	the plant defense responses	1731:1757	GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader.
34731210	11	48	theme	Curtobacterium	819:832	arg1	species					834:840	a new, still non described Curtobacterium species	792:840	a new, still non described Curtobacterium species	792:840	strains, with ANIb/ANIm ≥ 98%, representing a new, still non described Curtobacterium species.
34731210	6	49	theme	chitin	446:451	arg1	presence					424:431	the presence	420:431	the presence of colloidal chitin in the medium	420:465	The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium.
34731210	1	50	theme	genome	106:111	arg1	sequence					113:120	its genome sequence	102:120	its genome sequence	102:120	isolated from field-grown soybean and analysis of its genome sequence.
34731210	0	51	theme	sp	48:49	arg1	activity					17:24	The chitinolytic activity	0:24	The chitinolytic activity of the Curtobacterium sp	0:49	The chitinolytic activity of the Curtobacterium sp.
34731210	16	52	contain	has	1553:1555	arg2	tools					1561:1565	the tools	1557:1565	the tools for the hydrolysis of different polysaccharides	1557:1613	GD1 suggests that it has the tools for the hydrolysis of different polysaccharides.
34731210	16	52	contain	has	1553:1555	arg1	it					1550:1551	it	1550:1551	it	1550:1551	GD1 suggests that it has the tools for the hydrolysis of different polysaccharides.
34731210	15	53	from	abundance	1454:1462	arg1	genome					1503:1508	the genome	1499:1508	the genome of Curtobacterium sp	1499:1529	The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp.
34731210	1	54	theme	sequence	113:120	arg1	analysis					90:97	field-grown soybean and analysis	66:97	analysis	90:97	isolated from field-grown soybean and analysis of its genome sequence.
34731210	1	54	theme	sequence	113:120	arg1	soybean					78:84	field-grown soybean and analysis	66:97	soybean	78:84	isolated from field-grown soybean and analysis of its genome sequence.
34731210	12	55	theme	circular	847:854	arg1	visualization					863:875	The circular genome visualization	843:875	The circular genome visualization	843:875	The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved.
34731210	4	56	dep	noteworthy	221:230	arg1	sp					318:319	only Curtobacterium sp	298:319	only Curtobacterium sp	298:319	It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp.
34731210	4	57	attach	isolated	267:274	arg2	bacteria					247:254	all bacteria	243:254	all bacteria previously isolated from the same origin	243:295	It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp.
34731210	4	57	attach	isolated	267:274	arg1	origin					290:295	the same origin	281:295	the same origin	281:295	It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp.
34731210	8	58	theme	Curtobacterium	612:625	arg1	sp					627:628	Curtobacterium sp	612:628	Curtobacterium sp	612:628	Furthermore, Curtobacterium sp.
34731210	3	59	from	soybean	195:201	arg1	Brazil					206:211	Brazil	206:211	Brazil	206:211	GD1 was isolated from leaves of conventionally grown soybean in Brazil.
34731210	15	60	theme	Curtobacterium	1513:1526	arg1	sp					1528:1529	Curtobacterium sp	1513:1529	Curtobacterium sp	1513:1529	The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp.
34731210	6	61	theme	colloidal	436:444	arg1	chitin					446:451	colloidal chitin	436:451	colloidal chitin	436:451	The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium.
34731210	4	62	theme	Curtobacterium	303:316	arg1	sp					318:319	only Curtobacterium sp	298:319	only Curtobacterium sp	298:319	It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp.
34731210	15	63	theme	sp	1528:1529	arg1	genome					1503:1508	the genome	1499:1508	the genome of Curtobacterium sp	1499:1529	The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp.
34731210	14	64	theme	subsystem	1414:1422	arg1	products					1424:1431	auxin biosynthesis subsystem products	1395:1431	auxin biosynthesis subsystem products	1395:1431	Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified.
34731210	9	65	theme	GD1	631:633	arg1	genome					635:640	GD1 genome	631:640	GD1 genome	631:640	GD1 genome was sequenced and analyzed.
34731210	4	66	theme	only	298:301	arg1	sp					318:319	only Curtobacterium sp	298:319	only Curtobacterium sp	298:319	It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp.
34731210	12	67	theme	genome	856:861	arg1	visualization					863:875	The circular genome visualization	843:875	The circular genome visualization	843:875	The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved.
34731210	10	68	theme	phylogenetic	691:702	arg1	cluster					704:710	a phylogenetic cluster	689:710	a phylogenetic cluster with four other Curtobacterium spp	689:745	Our isolate formed a phylogenetic cluster with four other Curtobacterium spp.
34731210	2	69	theme	Curtobacterium	123:136	arg1	sp					138:139	Curtobacterium sp	123:139	Curtobacterium sp.	123:140	Curtobacterium sp.
34731210	13	70	theme	hydrolases	1117:1126	arg1	genes					1128:1132	glycosyl hydrolases genes	1108:1132	glycosyl hydrolases genes	1108:1132	The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed.
34731210	14	71	theme	toxic	1375:1379	arg1	compounds					1381:1389	toxic compounds	1375:1389	toxic compounds	1375:1389	Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified.
34731210	6	72	from	presence	424:431	arg1	medium					460:465	the medium	456:465	the medium	456:465	The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium.
32240739	8	0	theme	Congo	1104:1108	arg1	dye					1114:1116	Congo red dye	1104:1116	Congo red dye	1104:1116	Meanwhile, the thermal stability, mechanical properties of the aerogels and its adsorption property to Congo red dye could be improved directly by the addition of Fe3O4.
32240739	8	1	theme	aerogels	1064:1071	arg1	properties					1046:1055	mechanical properties	1035:1055	mechanical properties of the aerogels	1035:1071	Meanwhile, the thermal stability, mechanical properties of the aerogels and its adsorption property to Congo red dye could be improved directly by the addition of Fe3O4.
32240739	8	1	theme	aerogels	1064:1071	arg1	Meanwhile					1001:1009	Meanwhile	1001:1009	Meanwhile	1001:1009	Meanwhile, the thermal stability, mechanical properties of the aerogels and its adsorption property to Congo red dye could be improved directly by the addition of Fe3O4.
32240739	8	1	theme	aerogels	1064:1071	arg1	property					1092:1099	its adsorption property	1077:1099	its adsorption property	1077:1099	Meanwhile, the thermal stability, mechanical properties of the aerogels and its adsorption property to Congo red dye could be improved directly by the addition of Fe3O4.
32240739	8	1	theme	aerogels	1064:1071	arg1	stability					1024:1032	the thermal stability	1012:1032	the thermal stability	1012:1032	Meanwhile, the thermal stability, mechanical properties of the aerogels and its adsorption property to Congo red dye could be improved directly by the addition of Fe3O4.
32240739	6	2	theme	absorption	861:870	arg1	capacity					872:879	the maximum absorption capacity	849:879	the maximum absorption capacity of Congo red dye	849:896	The maximum compress strength of aerogel was 0.37 MPa, and the maximum absorption capacity of Congo red dye was 137.74 mg/g.
32240739	6	2	theme	absorption	861:870	arg1	137.74 mg/g					902:912	137.74 mg/g	902:912	137.74 mg/g	902:912	The maximum compress strength of aerogel was 0.37 MPa, and the maximum absorption capacity of Congo red dye was 137.74 mg/g.
32240739	1	3	theme	water	152:156	arg1	pollution					158:166	water pollution	152:166	water pollution	152:166	A renewable, efficient, and low-cost material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.
32240739	2	4	from	water	273:277	arg1	dyes					263:266	the dyes	259:266	the dyes from water based on hemicellulose	259:300	Here in, we demonstrate an aerogel to remove the dyes from water based on hemicellulose.
32240739	6	5	theme	maximum	853:859	arg1	capacity					872:879	the maximum absorption capacity	849:879	the maximum absorption capacity of Congo red dye	849:896	The maximum compress strength of aerogel was 0.37 MPa, and the maximum absorption capacity of Congo red dye was 137.74 mg/g.
32240739	6	5	theme	maximum	853:859	arg1	137.74 mg/g					902:912	137.74 mg/g	902:912	137.74 mg/g	902:912	The maximum compress strength of aerogel was 0.37 MPa, and the maximum absorption capacity of Congo red dye was 137.74 mg/g.
32240739	8	6	theme	red	1110:1112	arg1	dye					1114:1116	Congo red dye	1104:1116	Congo red dye	1104:1116	Meanwhile, the thermal stability, mechanical properties of the aerogels and its adsorption property to Congo red dye could be improved directly by the addition of Fe3O4.
32240739	5	7	from	C3	666:667	arg1	groups					649:654	hydroxyl groups	640:654	hydroxyl groups at C2 and C3 of hemicellulose	640:684	It was found that hydroxyl groups at C2 and C3 of hemicellulose were oxidized to aldehyde groups after modification, and the content of aldehyde group was 5.57 mmol/g.
32240739	3	8	theme	dialdehyde	307:316	arg1	DAH					334:336	DAH	334:336	DAH	334:336	The dialdehyde hemicelluloses (DAH) were synthesized by oxidation of hemicellulose extracted from straw with NaIO4.
32240739	3	8	theme	dialdehyde	307:316	arg1	hemicelluloses					318:331	The dialdehyde hemicelluloses	303:331	The dialdehyde hemicelluloses (DAH)	303:337	The dialdehyde hemicelluloses (DAH) were synthesized by oxidation of hemicellulose extracted from straw with NaIO4.
32240739	7	9	with	Aerogels	915:922	arg1	Fe3O4					929:933	Fe3O4	929:933	Fe3O4	929:933	Aerogels with Fe3O4 exhibited magnetism which enables the aerogels to easily recycle.
32240739	5	10	theme	aldehyde	758:765	arg1	group					767:771	aldehyde group	758:771	aldehyde group	758:771	It was found that hydroxyl groups at C2 and C3 of hemicellulose were oxidized to aldehyde groups after modification, and the content of aldehyde group was 5.57 mmol/g.
32240739	1	11	theme	renewable	81:89	arg1	material					116:123	A renewable, efficient, and low-cost material	79:123	A renewable, efficient, and low-cost material	79:123	A renewable, efficient, and low-cost material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.
32240739	1	11	theme	renewable	81:89	arg1	essential					128:136	essential	128:136	essential	128:136	A renewable, efficient, and low-cost material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.
32240739	5	12	theme	group	767:771	arg1	content					747:753	the content	743:753	the content of aldehyde group	743:771	It was found that hydroxyl groups at C2 and C3 of hemicellulose were oxidized to aldehyde groups after modification, and the content of aldehyde group was 5.57 mmol/g.
32240739	5	12	theme	group	767:771	arg1	5.57 mmol/g					777:787	5.57 mmol/g	777:787	5.57 mmol/g	777:787	It was found that hydroxyl groups at C2 and C3 of hemicellulose were oxidized to aldehyde groups after modification, and the content of aldehyde group was 5.57 mmol/g.
32240739	8	13	theme	thermal	1016:1022	arg1	stability					1024:1032	the thermal stability	1012:1032	the thermal stability	1012:1032	Meanwhile, the thermal stability, mechanical properties of the aerogels and its adsorption property to Congo red dye could be improved directly by the addition of Fe3O4.
32240739	6	14	theme	aerogel	823:829	arg1	0.37 MPa					835:842	0.37 MPa	835:842	0.37 MPa	835:842	The maximum compress strength of aerogel was 0.37 MPa, and the maximum absorption capacity of Congo red dye was 137.74 mg/g.
32240739	6	14	theme	aerogel	823:829	arg1	strength					811:818	The maximum compress strength	790:818	The maximum compress strength of aerogel	790:829	The maximum compress strength of aerogel was 0.37 MPa, and the maximum absorption capacity of Congo red dye was 137.74 mg/g.
32240739	8	15	theme	mechanical	1035:1044	arg1	properties					1046:1055	mechanical properties	1035:1055	mechanical properties of the aerogels	1035:1071	Meanwhile, the thermal stability, mechanical properties of the aerogels and its adsorption property to Congo red dye could be improved directly by the addition of Fe3O4.
32240739	1	16	theme	efficient	92:100	arg1	material					116:123	A renewable, efficient, and low-cost material	79:123	A renewable, efficient, and low-cost material	79:123	A renewable, efficient, and low-cost material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.
32240739	1	16	theme	efficient	92:100	arg1	essential					128:136	essential	128:136	essential	128:136	A renewable, efficient, and low-cost material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.
32240739	4	17	theme	base	530:533	arg1	reaction					535:542	the Schiff's base reaction	517:542	the Schiff's base reaction	517:542	The hydrogels were prepared based on the dialdehyde hemicellulose and chitosan-Fe3O4 composite by the Schiff's base reaction, which were processed with vacuum freeze-drying technique to obtain aerogels.
32240739	1	18	theme	heavy	186:190	arg1	ions					198:201	heavy metal ions	186:201	heavy metal ions	186:201	A renewable, efficient, and low-cost material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.
32240739	1	19	dep	such	169:172	arg1	as					174:175	as	174:175	as	174:175	A renewable, efficient, and low-cost material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.
32240739	2	20	dep	Here	214:217	arg1	in					219:220	in	219:220	in	219:220	Here in, we demonstrate an aerogel to remove the dyes from water based on hemicellulose.
32240739	3	21	theme	hemicellulose	372:384	arg1	oxidation					359:367	oxidation	359:367	oxidation of hemicellulose extracted from straw with NaIO4	359:416	The dialdehyde hemicelluloses (DAH) were synthesized by oxidation of hemicellulose extracted from straw with NaIO4.
32240739	5	22	theme	hemicellulose	672:684	arg1	C3					666:667	C3	666:667	C3	666:667	It was found that hydroxyl groups at C2 and C3 of hemicellulose were oxidized to aldehyde groups after modification, and the content of aldehyde group was 5.57 mmol/g.
32240739	5	22	theme	hemicellulose	672:684	arg1	C2					659:660	C2	659:660	C2	659:660	It was found that hydroxyl groups at C2 and C3 of hemicellulose were oxidized to aldehyde groups after modification, and the content of aldehyde group was 5.57 mmol/g.
32240739	5	23	theme	aldehyde	703:710	arg1	groups					712:717	aldehyde groups	703:717	aldehyde groups	703:717	It was found that hydroxyl groups at C2 and C3 of hemicellulose were oxidized to aldehyde groups after modification, and the content of aldehyde group was 5.57 mmol/g.
32240739	4	24	theme	chitosan-Fe3O4	489:502	arg1	composite					504:512	chitosan-Fe3O4 composite	489:512	chitosan-Fe3O4 composite	489:512	The hydrogels were prepared based on the dialdehyde hemicellulose and chitosan-Fe3O4 composite by the Schiff's base reaction, which were processed with vacuum freeze-drying technique to obtain aerogels.
32240739	5	25	theme	hydroxyl	640:647	arg1	groups					649:654	hydroxyl groups	640:654	hydroxyl groups at C2 and C3 of hemicellulose	640:684	It was found that hydroxyl groups at C2 and C3 of hemicellulose were oxidized to aldehyde groups after modification, and the content of aldehyde group was 5.57 mmol/g.
32240739	8	26	theme	Fe3O4	1164:1168	arg1	addition					1152:1159	the addition	1148:1159	the addition of Fe3O4	1148:1168	Meanwhile, the thermal stability, mechanical properties of the aerogels and its adsorption property to Congo red dye could be improved directly by the addition of Fe3O4.
32240739	6	27	theme	red	890:892	arg1	dye					894:896	Congo red dye	884:896	Congo red dye	884:896	The maximum compress strength of aerogel was 0.37 MPa, and the maximum absorption capacity of Congo red dye was 137.74 mg/g.
32240739	6	28	theme	compress	802:809	arg1	0.37 MPa					835:842	0.37 MPa	835:842	0.37 MPa	835:842	The maximum compress strength of aerogel was 0.37 MPa, and the maximum absorption capacity of Congo red dye was 137.74 mg/g.
32240739	6	28	theme	compress	802:809	arg1	strength					811:818	The maximum compress strength	790:818	The maximum compress strength of aerogel	790:829	The maximum compress strength of aerogel was 0.37 MPa, and the maximum absorption capacity of Congo red dye was 137.74 mg/g.
32240739	1	29	theme	low-cost	107:114	arg1	material					116:123	A renewable, efficient, and low-cost material	79:123	A renewable, efficient, and low-cost material	79:123	A renewable, efficient, and low-cost material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.
32240739	1	29	theme	low-cost	107:114	arg1	essential					128:136	essential	128:136	essential	128:136	A renewable, efficient, and low-cost material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.
32240739	6	30	theme	Congo	884:888	arg1	dye					894:896	Congo red dye	884:896	Congo red dye	884:896	The maximum compress strength of aerogel was 0.37 MPa, and the maximum absorption capacity of Congo red dye was 137.74 mg/g.
32240739	6	31	theme	maximum	794:800	arg1	0.37 MPa					835:842	0.37 MPa	835:842	0.37 MPa	835:842	The maximum compress strength of aerogel was 0.37 MPa, and the maximum absorption capacity of Congo red dye was 137.74 mg/g.
32240739	6	31	theme	maximum	794:800	arg1	strength					811:818	The maximum compress strength	790:818	The maximum compress strength of aerogel	790:829	The maximum compress strength of aerogel was 0.37 MPa, and the maximum absorption capacity of Congo red dye was 137.74 mg/g.
32240739	4	32	theme	vacuum	571:576	arg1	technique					592:600	vacuum freeze-drying technique	571:600	vacuum freeze-drying technique to obtain aerogels	571:619	The hydrogels were prepared based on the dialdehyde hemicellulose and chitosan-Fe3O4 composite by the Schiff's base reaction, which were processed with vacuum freeze-drying technique to obtain aerogels.
32240739	5	33	from	C2	659:660	arg1	groups					649:654	hydroxyl groups	640:654	hydroxyl groups at C2 and C3 of hemicellulose	640:684	It was found that hydroxyl groups at C2 and C3 of hemicellulose were oxidized to aldehyde groups after modification, and the content of aldehyde group was 5.57 mmol/g.
32240739	1	34	theme	metal	192:196	arg1	ions					198:201	heavy metal ions	186:201	heavy metal ions	186:201	A renewable, efficient, and low-cost material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.
32240739	1	35	dep	dyes	177:180	arg1	pollution					203:211	pollution	203:211	pollution	203:211	A renewable, efficient, and low-cost material is essential for adsorbing water pollution, such as dyes and heavy metal ions pollution.
32240739	8	36	theme	adsorption	1081:1090	arg1	property					1092:1099	its adsorption property	1077:1099	its adsorption property	1077:1099	Meanwhile, the thermal stability, mechanical properties of the aerogels and its adsorption property to Congo red dye could be improved directly by the addition of Fe3O4.
32240739	4	37	theme	dialdehyde	460:469	arg1	hemicellulose					471:483	the dialdehyde hemicellulose	456:483	the dialdehyde hemicellulose	456:483	The hydrogels were prepared based on the dialdehyde hemicellulose and chitosan-Fe3O4 composite by the Schiff's base reaction, which were processed with vacuum freeze-drying technique to obtain aerogels.
32240739	6	38	theme	dye	894:896	arg1	capacity					872:879	the maximum absorption capacity	849:879	the maximum absorption capacity of Congo red dye	849:896	The maximum compress strength of aerogel was 0.37 MPa, and the maximum absorption capacity of Congo red dye was 137.74 mg/g.
32240739	6	38	theme	dye	894:896	arg1	137.74 mg/g					902:912	137.74 mg/g	902:912	137.74 mg/g	902:912	The maximum compress strength of aerogel was 0.37 MPa, and the maximum absorption capacity of Congo red dye was 137.74 mg/g.
32240739	4	39	theme	freeze-drying	578:590	arg1	technique					592:600	vacuum freeze-drying technique	571:600	vacuum freeze-drying technique to obtain aerogels	571:619	The hydrogels were prepared based on the dialdehyde hemicellulose and chitosan-Fe3O4 composite by the Schiff's base reaction, which were processed with vacuum freeze-drying technique to obtain aerogels.
34655586	10	0	theme	H1299	1130:1134	arg1	cells					1146:1150	H1299 and MCF-7 cells	1130:1150	cells	1146:1150	LVF-I (250-1000 μg/mL) could inhibit the proliferation of H1299 and MCF-7 cells, while enhance the proliferative response of splenocyte and the phagocytic ability of RAW264.7.
34655586	12	1	theme	cells	1599:1603	arg1	proliferation					1576:1588	the proliferation	1572:1588	the proliferation of tumor cells	1572:1603	These results suggested that LVF-I had the potential to be developed as antitumor or immunomodulatory agents by inhibiting the proliferation of tumor cells and stimulating macrophages-mediated immune responses.
34655586	12	2	theme	macrophages-mediated	1621:1640	arg1	responses					1649:1657	macrophages-mediated immune responses	1621:1657	macrophages-mediated immune responses	1621:1657	These results suggested that LVF-I had the potential to be developed as antitumor or immunomodulatory agents by inhibiting the proliferation of tumor cells and stimulating macrophages-mediated immune responses.
34655586	10	3	theme	cells	1146:1150	arg1	proliferation					1113:1125	the proliferation	1109:1125	the proliferation of H1299 and MCF-7 cells	1109:1150	LVF-I (250-1000 μg/mL) could inhibit the proliferation of H1299 and MCF-7 cells, while enhance the proliferative response of splenocyte and the phagocytic ability of RAW264.7.
34655586	6	4	theme	inhibition	686:695	arg1	effect					697:702	its inhibition effect	682:702	its inhibition effect on H1299 and MCF-7 cells	682:727	Then the immunomodulation of LVF-I and its inhibition effect on H1299 and MCF-7 cells were investigated.
34655586	11	5	theme	interleukin-6	1337:1349	arg1	secretion					1310:1318	the secretion	1306:1318	the secretion of nitric oxide, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α)	1306:1392	Furthermore, LVF-I (250-1000 μg/mL) significantly induced the secretion of nitric oxide, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) by up-regulating their mRNA expression in macrophages.
34655586	0	6	theme	polysaccharide	93:106	arg1	activities					68:77	anti-proliferative and immunomodulatory activities	28:77	anti-proliferative and immunomodulatory activities of a purified polysaccharide from Lactarius volemus Fr	28:132	Structural characteristics, anti-proliferative and immunomodulatory activities of a purified polysaccharide from Lactarius volemus Fr.
34655586	0	6	theme	polysaccharide	93:106	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics	0:25	Structural characteristics, anti-proliferative and immunomodulatory activities of a purified polysaccharide from Lactarius volemus Fr.
34655586	10	7	theme	RAW264.7	1238:1245	arg1	response					1185:1192	the proliferative response	1167:1192	the proliferative response of splenocyte	1167:1206	LVF-I (250-1000 μg/mL) could inhibit the proliferation of H1299 and MCF-7 cells, while enhance the proliferative response of splenocyte and the phagocytic ability of RAW264.7.
34655586	10	7	theme	RAW264.7	1238:1245	arg1	ability					1227:1233	the phagocytic ability	1212:1233	the phagocytic ability of RAW264.7	1212:1245	LVF-I (250-1000 μg/mL) could inhibit the proliferation of H1299 and MCF-7 cells, while enhance the proliferative response of splenocyte and the phagocytic ability of RAW264.7.
34655586	5	8	theme	primary	448:454	arg1	structure					456:464	structure	456:464	structure	456:464	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	5	9	theme	purified	384:391	arg1	polysaccharide					393:406	A purified polysaccharide	382:406	A purified polysaccharide	382:406	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	5	9	theme	purified	384:391	arg1	LVF-I					436:440	LVF-I	436:440	LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy	436:640	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	0	10	theme	volemus	123:129	arg1	Fr					131:132	Lactarius volemus Fr	113:132	Lactarius volemus Fr	113:132	Structural characteristics, anti-proliferative and immunomodulatory activities of a purified polysaccharide from Lactarius volemus Fr.
34655586	3	11	theme	important	226:234	arg1	component					248:256	an important nutritional component	223:256	an important nutritional component of L. volemus	223:270	Polysaccharide is an important nutritional component of L. volemus.
34655586	3	11	theme	important	226:234	arg1	Polysaccharide					205:218	Polysaccharide	205:218	Polysaccharide	205:218	Polysaccharide is an important nutritional component of L. volemus.
34655586	10	12	theme	MCF-7	1140:1144	arg1	cells					1146:1150	H1299 and MCF-7 cells	1130:1150	cells	1146:1150	LVF-I (250-1000 μg/mL) could inhibit the proliferation of H1299 and MCF-7 cells, while enhance the proliferative response of splenocyte and the phagocytic ability of RAW264.7.
34655586	8	13	theme	1→	882:883	arg1	1→					940:941	→4)-α-D-Glcp-(1→, →6)-β-D-Manp-(1→, →6)-α-D-Galp-(1 → and →4)-β-D-Manp-(1→	868:941	1→	940:941	It had a backbone consisting of →4)-α-D-Glcp-(1→, →6)-β-D-Manp-(1→, →6)-α-D-Galp-(1 → and →4)-β-D-Manp-(1→.
34655586	0	14	theme	Lactarius	113:121	arg1	Fr					131:132	Lactarius volemus Fr	113:132	Lactarius volemus Fr	113:132	Structural characteristics, anti-proliferative and immunomodulatory activities of a purified polysaccharide from Lactarius volemus Fr.
34655586	12	15	theme	antitumor	1521:1529	arg1	agents					1551:1556	antitumor or immunomodulatory agents	1521:1556	antitumor or immunomodulatory agents	1521:1556	These results suggested that LVF-I had the potential to be developed as antitumor or immunomodulatory agents by inhibiting the proliferation of tumor cells and stimulating macrophages-mediated immune responses.
34655586	3	16	theme	nutritional	236:246	arg1	component					248:256	an important nutritional component	223:256	an important nutritional component of L. volemus	223:270	Polysaccharide is an important nutritional component of L. volemus.
34655586	3	16	theme	nutritional	236:246	arg1	Polysaccharide					205:218	Polysaccharide	205:218	Polysaccharide	205:218	Polysaccharide is an important nutritional component of L. volemus.
34655586	11	17	theme	mRNA	1417:1420	arg1	expression					1422:1431	their mRNA expression	1411:1431	their mRNA expression in macrophages	1411:1446	Furthermore, LVF-I (250-1000 μg/mL) significantly induced the secretion of nitric oxide, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) by up-regulating their mRNA expression in macrophages.
34655586	12	18	theme	immune	1642:1647	arg1	responses					1649:1657	macrophages-mediated immune responses	1621:1657	macrophages-mediated immune responses	1621:1657	These results suggested that LVF-I had the potential to be developed as antitumor or immunomodulatory agents by inhibiting the proliferation of tumor cells and stimulating macrophages-mediated immune responses.
34655586	5	19	theme	composition	535:545	arg1	results					509:515	the comprehensive results	491:515	the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy	491:640	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	8	20	theme	-β-D-Manp-	889:898	arg1	1→					940:941	→4)-α-D-Glcp-(1→, →6)-β-D-Manp-(1→, →6)-α-D-Galp-(1 → and →4)-β-D-Manp-(1→	868:941	1→	940:941	It had a backbone consisting of →4)-α-D-Glcp-(1→, →6)-β-D-Manp-(1→, →6)-α-D-Galp-(1 → and →4)-β-D-Manp-(1→.
34655586	12	21	theme	tumor	1593:1597	arg1	cells					1599:1603	tumor cells	1593:1603	tumor cells	1593:1603	These results suggested that LVF-I had the potential to be developed as antitumor or immunomodulatory agents by inhibiting the proliferation of tumor cells and stimulating macrophages-mediated immune responses.
34655586	2	22	theme	edible	163:168	arg1	mushroom					170:177	an edible mushroom	160:177	an edible mushroom widely consumed in China	160:202	is an edible mushroom widely consumed in China.
34655586	8	23	contain	had	839:841	arg2	backbone					845:852	a backbone	843:852	a backbone consisting of →4)-α-D-Glcp-(1→, →6)-β-D-Manp-(1→, →6)-α-D-Galp-(1 → and →4)-β-D-Manp-(1→	843:941	It had a backbone consisting of →4)-α-D-Glcp-(1→, →6)-β-D-Manp-(1→, →6)-α-D-Galp-(1 → and →4)-β-D-Manp-(1→.
34655586	8	23	contain	had	839:841	arg1	It					836:837	It	836:837	It	836:837	It had a backbone consisting of →4)-α-D-Glcp-(1→, →6)-β-D-Manp-(1→, →6)-α-D-Galp-(1 → and →4)-β-D-Manp-(1→.
34655586	9	24	theme	-α-D-Galp-	1011:1020	arg1	1→					1068:1069	→4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→	987:1069	1→	1068:1069	And its side chains were branched at C2 of →4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→.
34655586	5	25	theme	NMR	625:627	arg1	spectroscopy					629:640	FT-IR and 1D/2D NMR spectroscopy	609:640	spectroscopy	629:640	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	5	25	theme	NMR	625:627	arg1	composition					535:545	monosaccharide composition	520:545	monosaccharide composition	520:545	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	5	26	theme	periodate	548:556	arg1	degradation					574:584	periodate oxidation-smith degradation	548:584	periodate oxidation-smith degradation	548:584	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	5	26	theme	periodate	548:556	arg1	composition					535:545	monosaccharide composition	520:545	monosaccharide composition	520:545	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	6	27	theme	LVF-I	672:676	arg1	immunomodulation					652:667	Then the immunomodulation	643:667	Then the immunomodulation of LVF-I	643:676	Then the immunomodulation of LVF-I and its inhibition effect on H1299 and MCF-7 cells were investigated.
34655586	6	27	theme	LVF-I	672:676	arg1	effect					697:702	its inhibition effect	682:702	its inhibition effect on H1299 and MCF-7 cells	682:727	Then the immunomodulation of LVF-I and its inhibition effect on H1299 and MCF-7 cells were investigated.
34655586	3	28	theme	L.	261:262	arg1	volemus					264:270	L. volemus	261:270	L. volemus	261:270	Polysaccharide is an important nutritional component of L. volemus.
34655586	5	29	theme	oxidation-smith	558:572	arg1	degradation					574:584	periodate oxidation-smith degradation	548:584	periodate oxidation-smith degradation	548:584	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	5	29	theme	oxidation-smith	558:572	arg1	composition					535:545	monosaccharide composition	520:545	monosaccharide composition	520:545	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	0	30	from	activities	68:77	arg1	Fr					131:132	Lactarius volemus Fr	113:132	Lactarius volemus Fr	113:132	Structural characteristics, anti-proliferative and immunomodulatory activities of a purified polysaccharide from Lactarius volemus Fr.
34655586	0	31	theme	Structural	0:9	arg1	activities					68:77	anti-proliferative and immunomodulatory activities	28:77	anti-proliferative and immunomodulatory activities of a purified polysaccharide from Lactarius volemus Fr	28:132	Structural characteristics, anti-proliferative and immunomodulatory activities of a purified polysaccharide from Lactarius volemus Fr.
34655586	0	31	theme	Structural	0:9	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics	0:25	Structural characteristics, anti-proliferative and immunomodulatory activities of a purified polysaccharide from Lactarius volemus Fr.
34655586	3	32	theme	volemus	264:270	arg1	component					248:256	an important nutritional component	223:256	an important nutritional component of L. volemus	223:270	Polysaccharide is an important nutritional component of L. volemus.
34655586	3	32	theme	volemus	264:270	arg1	Polysaccharide					205:218	Polysaccharide	205:218	Polysaccharide	205:218	Polysaccharide is an important nutritional component of L. volemus.
34655586	5	33	theme	comprehensive	495:507	arg1	results					509:515	the comprehensive results	491:515	the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy	491:640	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	0	34	theme	anti-proliferative	28:45	arg1	activities					68:77	anti-proliferative and immunomodulatory activities	28:77	anti-proliferative and immunomodulatory activities of a purified polysaccharide from Lactarius volemus Fr	28:132	Structural characteristics, anti-proliferative and immunomodulatory activities of a purified polysaccharide from Lactarius volemus Fr.
34655586	0	34	theme	anti-proliferative	28:45	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics	0:25	Structural characteristics, anti-proliferative and immunomodulatory activities of a purified polysaccharide from Lactarius volemus Fr.
34655586	9	35	theme	α-L-Fucp-	1058:1066	arg1	1→					1068:1069	→4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→	987:1069	1→	1068:1069	And its side chains were branched at C2 of →4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→.
34655586	11	36	theme	tumor	1362:1366	arg1	TNF-α					1387:1391	TNF-α	1387:1391	TNF-α	1387:1391	Furthermore, LVF-I (250-1000 μg/mL) significantly induced the secretion of nitric oxide, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) by up-regulating their mRNA expression in macrophages.
34655586	11	36	theme	tumor	1362:1366	arg1	factor-α					1377:1384	tumor necrosis factor-α	1362:1384	tumor necrosis factor-α (TNF-α)	1362:1392	Furthermore, LVF-I (250-1000 μg/mL) significantly induced the secretion of nitric oxide, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) by up-regulating their mRNA expression in macrophages.
34655586	9	37	theme	side	952:955	arg1	chains					957:962	its side chains	948:962	its side chains	948:962	And its side chains were branched at C2 of →4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→.
34655586	9	38	theme	1→	1022:1023	arg1	1→					1068:1069	→4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→	987:1069	1→	1068:1069	And its side chains were branched at C2 of →4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→.
34655586	6	39	from	immunomodulation	652:667	arg1	cells					723:727	H1299 and MCF-7 cells	707:727	cells	723:727	Then the immunomodulation of LVF-I and its inhibition effect on H1299 and MCF-7 cells were investigated.
34655586	9	40	theme	1→	1036:1037	arg1	1→					1068:1069	→4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→	987:1069	1→	1068:1069	And its side chains were branched at C2 of →4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→.
34655586	12	41	theme	immunomodulatory	1534:1549	arg1	agents					1551:1556	antitumor or immunomodulatory agents	1521:1556	antitumor or immunomodulatory agents	1521:1556	These results suggested that LVF-I had the potential to be developed as antitumor or immunomodulatory agents by inhibiting the proliferation of tumor cells and stimulating macrophages-mediated immune responses.
34655586	9	42	dep	1→	1068:1069	arg1	α-D-Glcp-					1026:1034	→4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→	987:1069	α-D-Glcp-	1026:1034	And its side chains were branched at C2 of →4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→.
34655586	9	42	dep	1→	1068:1069	arg1	α-D-Galp-					1040:1048	→4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→	987:1069	α-D-Galp-	1040:1048	And its side chains were branched at C2 of →4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→.
34655586	0	43	theme	immunomodulatory	51:66	arg1	activities					68:77	anti-proliferative and immunomodulatory activities	28:77	anti-proliferative and immunomodulatory activities of a purified polysaccharide from Lactarius volemus Fr	28:132	Structural characteristics, anti-proliferative and immunomodulatory activities of a purified polysaccharide from Lactarius volemus Fr.
34655586	0	43	theme	immunomodulatory	51:66	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics	0:25	Structural characteristics, anti-proliferative and immunomodulatory activities of a purified polysaccharide from Lactarius volemus Fr.
34655586	9	44	theme	1 → by	1001:1006	arg1	1→					1068:1069	→4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→	987:1069	1→	1068:1069	And its side chains were branched at C2 of →4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→.
34655586	8	45	theme	-α-D-Galp-	907:916	arg1	1→					940:941	→4)-α-D-Glcp-(1→, →6)-β-D-Manp-(1→, →6)-α-D-Galp-(1 → and →4)-β-D-Manp-(1→	868:941	1→	940:941	It had a backbone consisting of →4)-α-D-Glcp-(1→, →6)-β-D-Manp-(1→, →6)-α-D-Galp-(1 → and →4)-β-D-Manp-(1→.
34655586	5	46	theme	monosaccharide	520:533	arg1	degradation					574:584	periodate oxidation-smith degradation	548:584	periodate oxidation-smith degradation	548:584	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	5	46	theme	monosaccharide	520:533	arg1	analysis					599:606	methylation analysis	587:606	methylation analysis	587:606	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	5	46	theme	monosaccharide	520:533	arg1	spectroscopy					629:640	FT-IR and 1D/2D NMR spectroscopy	609:640	spectroscopy	629:640	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	5	46	theme	monosaccharide	520:533	arg1	composition					535:545	monosaccharide composition	520:545	monosaccharide composition	520:545	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	9	47	theme	1 → and	1050:1056	arg1	1→					1068:1069	→4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→	987:1069	1→	1068:1069	And its side chains were branched at C2 of →4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→.
34655586	8	48	dep	1→	940:941	arg1	→6					886:887	→4)-α-D-Glcp-(1→, →6)-β-D-Manp-(1→, →6)-α-D-Galp-(1 → and →4)-β-D-Manp-(1→	868:941	→6	886:887	It had a backbone consisting of →4)-α-D-Glcp-(1→, →6)-β-D-Manp-(1→, →6)-α-D-Galp-(1 → and →4)-β-D-Manp-(1→.
34655586	11	49	theme	necrosis	1368:1375	arg1	TNF-α					1387:1391	TNF-α	1387:1391	TNF-α	1387:1391	Furthermore, LVF-I (250-1000 μg/mL) significantly induced the secretion of nitric oxide, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) by up-regulating their mRNA expression in macrophages.
34655586	11	49	theme	necrosis	1368:1375	arg1	factor-α					1377:1384	tumor necrosis factor-α	1362:1384	tumor necrosis factor-α (TNF-α)	1362:1392	Furthermore, LVF-I (250-1000 μg/mL) significantly induced the secretion of nitric oxide, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) by up-regulating their mRNA expression in macrophages.
34655586	12	50	contain	had	1484:1486	arg1	LVF-I					1478:1482	LVF-I	1478:1482	LVF-I	1478:1482	These results suggested that LVF-I had the potential to be developed as antitumor or immunomodulatory agents by inhibiting the proliferation of tumor cells and stimulating macrophages-mediated immune responses.
34655586	12	50	contain	had	1484:1486	arg2	potential					1492:1500	the potential to be developed as antitumor or immunomodulatory agents by inhibiting the proliferation of tumor cells and stimulating macrophages-mediated immune responses	1488:1657	the potential to be developed as antitumor or immunomodulatory agents by inhibiting the proliferation of tumor cells and stimulating macrophages-mediated immune responses	1488:1657	These results suggested that LVF-I had the potential to be developed as antitumor or immunomodulatory agents by inhibiting the proliferation of tumor cells and stimulating macrophages-mediated immune responses.
34655586	10	51	theme	splenocyte	1197:1206	arg1	response					1185:1192	the proliferative response	1167:1192	the proliferative response of splenocyte	1167:1206	LVF-I (250-1000 μg/mL) could inhibit the proliferation of H1299 and MCF-7 cells, while enhance the proliferative response of splenocyte and the phagocytic ability of RAW264.7.
34655586	10	51	theme	splenocyte	1197:1206	arg1	ability					1227:1233	the phagocytic ability	1212:1233	the phagocytic ability of RAW264.7	1212:1245	LVF-I (250-1000 μg/mL) could inhibit the proliferation of H1299 and MCF-7 cells, while enhance the proliferative response of splenocyte and the phagocytic ability of RAW264.7.
34655586	7	52	contain	contained	786:794	arg1	LVF-I					768:772	LVF-I	768:772	LVF-I (12,894 Da)	768:784	Results showed that LVF-I (12,894 Da) contained fucose, mannose, glucose and galactose.
34655586	7	52	contain	contained	786:794	arg2	glucose					813:819	glucose	813:819	glucose	813:819	Results showed that LVF-I (12,894 Da) contained fucose, mannose, glucose and galactose.
34655586	7	52	contain	contained	786:794	arg2	fucose					796:801	fucose	796:801	fucose	796:801	Results showed that LVF-I (12,894 Da) contained fucose, mannose, glucose and galactose.
34655586	7	52	contain	contained	786:794	arg2	galactose					825:833	galactose	825:833	galactose	825:833	Results showed that LVF-I (12,894 Da) contained fucose, mannose, glucose and galactose.
34655586	7	52	contain	contained	786:794	arg2	mannose					804:810	mannose	804:810	mannose	804:810	Results showed that LVF-I (12,894 Da) contained fucose, mannose, glucose and galactose.
34655586	7	52	contain	contained	786:794	arg1	12,894 Da					775:783	12,894 Da	775:783	12,894 Da	775:783	Results showed that LVF-I (12,894 Da) contained fucose, mannose, glucose and galactose.
34655586	5	53	theme	FT-IR	609:613	arg1	spectroscopy					629:640	FT-IR and 1D/2D NMR spectroscopy	609:640	spectroscopy	629:640	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	5	53	theme	FT-IR	609:613	arg1	composition					535:545	monosaccharide composition	520:545	monosaccharide composition	520:545	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	1	54	theme	Lactarius	135:143	arg1	Fr					153:154	Lactarius volemus Fr	135:154	Lactarius volemus Fr.	135:155	Lactarius volemus Fr.
34655586	11	55	theme	oxide	1330:1334	arg1	secretion					1310:1318	the secretion	1306:1318	the secretion of nitric oxide, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α)	1306:1392	Furthermore, LVF-I (250-1000 μg/mL) significantly induced the secretion of nitric oxide, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) by up-regulating their mRNA expression in macrophages.
34655586	8	56	theme	→4	926:927	arg1	1→					940:941	→4)-α-D-Glcp-(1→, →6)-β-D-Manp-(1→, →6)-α-D-Galp-(1 → and →4)-β-D-Manp-(1→	868:941	1→	940:941	It had a backbone consisting of →4)-α-D-Glcp-(1→, →6)-β-D-Manp-(1→, →6)-α-D-Galp-(1 → and →4)-β-D-Manp-(1→.
34655586	1	57	theme	volemus	145:151	arg1	Fr					153:154	Lactarius volemus Fr	135:154	Lactarius volemus Fr.	135:155	Lactarius volemus Fr.
34655586	6	58	theme	MCF-7	717:721	arg1	cells					723:727	H1299 and MCF-7 cells	707:727	cells	723:727	Then the immunomodulation of LVF-I and its inhibition effect on H1299 and MCF-7 cells were investigated.
34655586	0	59	from	Fr	131:132	arg1	polysaccharide					93:106	a purified polysaccharide	82:106	a purified polysaccharide from Lactarius volemus Fr	82:132	Structural characteristics, anti-proliferative and immunomodulatory activities of a purified polysaccharide from Lactarius volemus Fr.
34655586	0	59	from	Fr	131:132	arg1	activities					68:77	anti-proliferative and immunomodulatory activities	28:77	anti-proliferative and immunomodulatory activities of a purified polysaccharide from Lactarius volemus Fr	28:132	Structural characteristics, anti-proliferative and immunomodulatory activities of a purified polysaccharide from Lactarius volemus Fr.
34655586	0	59	from	Fr	131:132	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics	0:25	Structural characteristics, anti-proliferative and immunomodulatory activities of a purified polysaccharide from Lactarius volemus Fr.
34655586	5	60	theme	1D/2D	619:623	arg1	spectroscopy					629:640	FT-IR and 1D/2D NMR spectroscopy	609:640	spectroscopy	629:640	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	5	60	theme	1D/2D	619:623	arg1	composition					535:545	monosaccharide composition	520:545	monosaccharide composition	520:545	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	9	61	theme	1→	1068:1069	arg1	C2					981:982	C2	981:982	C2 of →4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→	981:1069	And its side chains were branched at C2 of →4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→.
34655586	8	62	theme	1 → and	918:924	arg1	1→					940:941	→4)-α-D-Glcp-(1→, →6)-β-D-Manp-(1→, →6)-α-D-Galp-(1 → and →4)-β-D-Manp-(1→	868:941	1→	940:941	It had a backbone consisting of →4)-α-D-Glcp-(1→, →6)-β-D-Manp-(1→, →6)-α-D-Galp-(1 → and →4)-β-D-Manp-(1→.
34655586	8	63	theme	-β-D-Manp-	929:938	arg1	1→					940:941	→4)-α-D-Glcp-(1→, →6)-β-D-Manp-(1→, →6)-α-D-Galp-(1 → and →4)-β-D-Manp-(1→	868:941	1→	940:941	It had a backbone consisting of →4)-α-D-Glcp-(1→, →6)-β-D-Manp-(1→, →6)-α-D-Galp-(1 → and →4)-β-D-Manp-(1→.
34655586	11	64	from	expression	1422:1431	arg1	macrophages					1436:1446	macrophages	1436:1446	macrophages	1436:1446	Furthermore, LVF-I (250-1000 μg/mL) significantly induced the secretion of nitric oxide, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) by up-regulating their mRNA expression in macrophages.
34655586	6	65	theme	H1299	707:711	arg1	cells					723:727	H1299 and MCF-7 cells	707:727	cells	723:727	Then the immunomodulation of LVF-I and its inhibition effect on H1299 and MCF-7 cells were investigated.
34655586	10	66	theme	proliferative	1171:1183	arg1	response					1185:1192	the proliferative response	1167:1192	the proliferative response of splenocyte	1167:1206	LVF-I (250-1000 μg/mL) could inhibit the proliferation of H1299 and MCF-7 cells, while enhance the proliferative response of splenocyte and the phagocytic ability of RAW264.7.
34655586	0	67	theme	purified	84:91	arg1	polysaccharide					93:106	a purified polysaccharide	82:106	a purified polysaccharide from Lactarius volemus Fr	82:132	Structural characteristics, anti-proliferative and immunomodulatory activities of a purified polysaccharide from Lactarius volemus Fr.
34655586	9	68	theme	→6	1008:1009	arg1	1→					1068:1069	→4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→	987:1069	1→	1068:1069	And its side chains were branched at C2 of →4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→.
34655586	6	69	from	effect	697:702	arg1	cells					723:727	H1299 and MCF-7 cells	707:727	cells	723:727	Then the immunomodulation of LVF-I and its inhibition effect on H1299 and MCF-7 cells were investigated.
34655586	10	70	theme	phagocytic	1216:1225	arg1	ability					1227:1233	the phagocytic ability	1212:1233	the phagocytic ability of RAW264.7	1212:1245	LVF-I (250-1000 μg/mL) could inhibit the proliferation of H1299 and MCF-7 cells, while enhance the proliferative response of splenocyte and the phagocytic ability of RAW264.7.
34655586	11	71	theme	factor-α	1377:1384	arg1	secretion					1310:1318	the secretion	1306:1318	the secretion of nitric oxide, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α)	1306:1392	Furthermore, LVF-I (250-1000 μg/mL) significantly induced the secretion of nitric oxide, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) by up-regulating their mRNA expression in macrophages.
34655586	4	72	from	volemus	331:337	arg1	polysaccharide					308:321	the polysaccharide	304:321	the polysaccharide from L. volemus	304:337	This research aimed to isolate the polysaccharide from L. volemus and study its structure and bioactivities.
34655586	5	73	theme	methylation	587:597	arg1	analysis					599:606	methylation analysis	587:606	methylation analysis	587:606	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	5	73	theme	methylation	587:597	arg1	composition					535:545	monosaccharide composition	520:545	monosaccharide composition	520:545	A purified polysaccharide was identified and named as LVF-I whose primary structure was proposed considering the comprehensive results of monosaccharide composition, periodate oxidation-smith degradation, methylation analysis, FT-IR and 1D/2D NMR spectroscopy.
34655586	8	74	theme	-α-D-Glcp-	871:880	arg1	1→					940:941	→4)-α-D-Glcp-(1→, →6)-β-D-Manp-(1→, →6)-α-D-Galp-(1 → and →4)-β-D-Manp-(1→	868:941	1→	940:941	It had a backbone consisting of →4)-α-D-Glcp-(1→, →6)-β-D-Manp-(1→, →6)-α-D-Galp-(1 → and →4)-β-D-Manp-(1→.
34655586	9	75	theme	-β-D-Manp-	990:999	arg1	1→					1068:1069	→4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→	987:1069	1→	1068:1069	And its side chains were branched at C2 of →4)-β-D-Manp-(1 → by →6)-α-D-Galp-(1→, α-D-Glcp-(1→, α-D-Galp-(1 → and α-L-Fucp-(1→.
34655586	11	76	theme	nitric	1323:1328	arg1	oxide					1330:1334	nitric oxide	1323:1334	nitric oxide	1323:1334	Furthermore, LVF-I (250-1000 μg/mL) significantly induced the secretion of nitric oxide, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) by up-regulating their mRNA expression in macrophages.
34198036	0	0	from	characterization	16:31	arg1	preservation					163:174	strawberry preservation	152:174	strawberry preservation	152:174	Preparation and characterization of k-carrageenan/konjac glucomannan/TiO2 nanocomposite film with efficient anti-fungal activity and its application in strawberry preservation.
34198036	2	1	theme	based	361:365	arg1	films					382:386	the KC/KGM based nano-composite films	350:386	the KC/KGM based nano-composite films prepared from different weight ratio of TiO2 NPs	350:435	The morphology and structure of the KC/KGM based nano-composite films prepared from different weight ratio of TiO2 NPs were characterized by SEM, XRD and FT-IR techniques.
34198036	1	2	theme	TiO2	266:269	arg1	nanoparticles					271:283	TiO2 nanoparticles	266:283	TiO2 nanoparticles (NPs)	266:289	A nano-composite film was prepared from k-carrageenan (KC), konjac glucomannan (KGM) and TiO2 nanoparticles (NPs) by solvent casting method.
34198036	1	2	theme	TiO2	266:269	arg1	NPs					286:288	NPs	286:288	NPs	286:288	A nano-composite film was prepared from k-carrageenan (KC), konjac glucomannan (KGM) and TiO2 nanoparticles (NPs) by solvent casting method.
34198036	0	3	theme	anti-fungal	108:118	arg1	activity					120:127	efficient anti-fungal activity	98:127	efficient anti-fungal activity	98:127	Preparation and characterization of k-carrageenan/konjac glucomannan/TiO2 nanocomposite film with efficient anti-fungal activity and its application in strawberry preservation.
34198036	2	4	theme	KC/KGM	354:359	arg1	films					382:386	the KC/KGM based nano-composite films	350:386	the KC/KGM based nano-composite films prepared from different weight ratio of TiO2 NPs	350:435	The morphology and structure of the KC/KGM based nano-composite films prepared from different weight ratio of TiO2 NPs were characterized by SEM, XRD and FT-IR techniques.
34198036	0	5	from	Preparation	0:10	arg1	preservation					163:174	strawberry preservation	152:174	strawberry preservation	152:174	Preparation and characterization of k-carrageenan/konjac glucomannan/TiO2 nanocomposite film with efficient anti-fungal activity and its application in strawberry preservation.
34198036	3	6	theme	NPs	511:513	arg1	addition					494:501	The addition	490:501	The addition of TiO2 NPs to KC/KGM films	490:529	The addition of TiO2 NPs to KC/KGM films improved the mechanical, thermal, structural, and barrier properties of nano-composite films.
34198036	4	7	theme	film	644:647	arg1	%					664:664	the film containing 7 wt%	640:664	the film containing 7 wt% of TiO2 NPs	640:676	In particular, the film containing 7 wt% of TiO2 NPs exhibited effective photocatalytic anti-fungal activity (79%) for Penicillium viridicatum after irradiating 6 h through generating reactive oxygen species thus destroying the fungi.
34198036	4	7	theme	film	644:647	arg1	NPs					674:676	TiO2 NPs	669:676	TiO2 NPs	669:676	In particular, the film containing 7 wt% of TiO2 NPs exhibited effective photocatalytic anti-fungal activity (79%) for Penicillium viridicatum after irradiating 6 h through generating reactive oxygen species thus destroying the fungi.
34198036	5	8	theme	strawberry	980:989	arg1	storage					991:997	strawberry storage	980:997	strawberry storage	980:997	Benefitting from the improved characteristics investigated above, the nano-composite films showed a favorable effect on strawberry storage.
34198036	4	9	theme	NPs	674:676	arg1	%					664:664	the film containing 7 wt%	640:664	the film containing 7 wt% of TiO2 NPs	640:676	In particular, the film containing 7 wt% of TiO2 NPs exhibited effective photocatalytic anti-fungal activity (79%) for Penicillium viridicatum after irradiating 6 h through generating reactive oxygen species thus destroying the fungi.
34198036	4	9	theme	NPs	674:676	arg1	NPs					674:676	TiO2 NPs	669:676	TiO2 NPs	669:676	In particular, the film containing 7 wt% of TiO2 NPs exhibited effective photocatalytic anti-fungal activity (79%) for Penicillium viridicatum after irradiating 6 h through generating reactive oxygen species thus destroying the fungi.
34198036	4	10	theme	TiO2	669:672	arg1	NPs					674:676	TiO2 NPs	669:676	TiO2 NPs	669:676	In particular, the film containing 7 wt% of TiO2 NPs exhibited effective photocatalytic anti-fungal activity (79%) for Penicillium viridicatum after irradiating 6 h through generating reactive oxygen species thus destroying the fungi.
34198036	6	11	theme	food	1098:1101	arg1	applications					1130:1141	food preservation and packaging applications	1098:1141	food preservation and packaging applications	1098:1141	Overall, the fabricated KC/KGM/TiO2 nano-composite films have a broad application in the field of food preservation and packaging applications.
34198036	3	12	theme	KC/KGM	518:523	arg1	films					525:529	KC/KGM films	518:529	KC/KGM films	518:529	The addition of TiO2 NPs to KC/KGM films improved the mechanical, thermal, structural, and barrier properties of nano-composite films.
34198036	5	13	theme	nano-composite	930:943	arg1	films					945:949	the nano-composite films	926:949	the nano-composite films	926:949	Benefitting from the improved characteristics investigated above, the nano-composite films showed a favorable effect on strawberry storage.
34198036	2	14	theme	weight	412:417	arg1	ratio					419:423	different weight ratio	402:423	different weight ratio of TiO2 NPs	402:435	The morphology and structure of the KC/KGM based nano-composite films prepared from different weight ratio of TiO2 NPs were characterized by SEM, XRD and FT-IR techniques.
34198036	2	15	theme	FT-IR	472:476	arg1	techniques					478:487	FT-IR techniques	472:487	FT-IR techniques	472:487	The morphology and structure of the KC/KGM based nano-composite films prepared from different weight ratio of TiO2 NPs were characterized by SEM, XRD and FT-IR techniques.
34198036	6	16	contain	have	1057:1060	arg1	films					1051:1055	the fabricated KC/KGM/TiO2 nano-composite films	1009:1055	the fabricated KC/KGM/TiO2 nano-composite films	1009:1055	Overall, the fabricated KC/KGM/TiO2 nano-composite films have a broad application in the field of food preservation and packaging applications.
34198036	6	16	contain	have	1057:1060	arg2	application					1070:1080	a broad application	1062:1080	a broad application	1062:1080	Overall, the fabricated KC/KGM/TiO2 nano-composite films have a broad application in the field of food preservation and packaging applications.
34198036	6	17	theme	preservation	1103:1114	arg1	applications					1130:1141	food preservation and packaging applications	1098:1141	food preservation and packaging applications	1098:1141	Overall, the fabricated KC/KGM/TiO2 nano-composite films have a broad application in the field of food preservation and packaging applications.
34198036	2	18	theme	films	382:386	arg1	structure					337:345	structure	337:345	structure	337:345	The morphology and structure of the KC/KGM based nano-composite films prepared from different weight ratio of TiO2 NPs were characterized by SEM, XRD and FT-IR techniques.
34198036	2	18	theme	films	382:386	arg1	morphology					322:331	morphology	322:331	morphology	322:331	The morphology and structure of the KC/KGM based nano-composite films prepared from different weight ratio of TiO2 NPs were characterized by SEM, XRD and FT-IR techniques.
34198036	4	19	theme	7 wt	660:663	arg1	%					664:664	the film containing 7 wt%	640:664	the film containing 7 wt% of TiO2 NPs	640:676	In particular, the film containing 7 wt% of TiO2 NPs exhibited effective photocatalytic anti-fungal activity (79%) for Penicillium viridicatum after irradiating 6 h through generating reactive oxygen species thus destroying the fungi.
34198036	4	19	theme	7 wt	660:663	arg1	NPs					674:676	TiO2 NPs	669:676	TiO2 NPs	669:676	In particular, the film containing 7 wt% of TiO2 NPs exhibited effective photocatalytic anti-fungal activity (79%) for Penicillium viridicatum after irradiating 6 h through generating reactive oxygen species thus destroying the fungi.
34198036	6	20	theme	fabricated	1013:1022	arg1	films					1051:1055	the fabricated KC/KGM/TiO2 nano-composite films	1009:1055	the fabricated KC/KGM/TiO2 nano-composite films	1009:1055	Overall, the fabricated KC/KGM/TiO2 nano-composite films have a broad application in the field of food preservation and packaging applications.
34198036	4	21	theme	containing	649:658	arg1	%					664:664	the film containing 7 wt%	640:664	the film containing 7 wt% of TiO2 NPs	640:676	In particular, the film containing 7 wt% of TiO2 NPs exhibited effective photocatalytic anti-fungal activity (79%) for Penicillium viridicatum after irradiating 6 h through generating reactive oxygen species thus destroying the fungi.
34198036	4	21	theme	containing	649:658	arg1	NPs					674:676	TiO2 NPs	669:676	TiO2 NPs	669:676	In particular, the film containing 7 wt% of TiO2 NPs exhibited effective photocatalytic anti-fungal activity (79%) for Penicillium viridicatum after irradiating 6 h through generating reactive oxygen species thus destroying the fungi.
34198036	5	22	theme	favorable	960:968	arg1	effect					970:975	a favorable effect	958:975	a favorable effect on strawberry storage	958:997	Benefitting from the improved characteristics investigated above, the nano-composite films showed a favorable effect on strawberry storage.
34198036	2	23	theme	NPs	433:435	arg1	ratio					419:423	different weight ratio	402:423	different weight ratio of TiO2 NPs	402:435	The morphology and structure of the KC/KGM based nano-composite films prepared from different weight ratio of TiO2 NPs were characterized by SEM, XRD and FT-IR techniques.
34198036	1	24	theme	solvent	294:300	arg1	method					310:315	solvent casting method	294:315	solvent casting method	294:315	A nano-composite film was prepared from k-carrageenan (KC), konjac glucomannan (KGM) and TiO2 nanoparticles (NPs) by solvent casting method.
34198036	0	25	theme	strawberry	152:161	arg1	preservation					163:174	strawberry preservation	152:174	strawberry preservation	152:174	Preparation and characterization of k-carrageenan/konjac glucomannan/TiO2 nanocomposite film with efficient anti-fungal activity and its application in strawberry preservation.
34198036	3	26	theme	TiO2	506:509	arg1	NPs					511:513	TiO2 NPs	506:513	TiO2 NPs	506:513	The addition of TiO2 NPs to KC/KGM films improved the mechanical, thermal, structural, and barrier properties of nano-composite films.
34198036	2	27	theme	TiO2	428:431	arg1	NPs					433:435	TiO2 NPs	428:435	TiO2 NPs	428:435	The morphology and structure of the KC/KGM based nano-composite films prepared from different weight ratio of TiO2 NPs were characterized by SEM, XRD and FT-IR techniques.
34198036	0	28	theme	glucomannan/TiO2	57:72	arg1	film					88:91	k-carrageenan/konjac glucomannan/TiO2 nanocomposite film	36:91	k-carrageenan/konjac glucomannan/TiO2 nanocomposite film with efficient anti-fungal activity	36:127	Preparation and characterization of k-carrageenan/konjac glucomannan/TiO2 nanocomposite film with efficient anti-fungal activity and its application in strawberry preservation.
34198036	1	29	theme	casting	302:308	arg1	method					310:315	solvent casting method	294:315	solvent casting method	294:315	A nano-composite film was prepared from k-carrageenan (KC), konjac glucomannan (KGM) and TiO2 nanoparticles (NPs) by solvent casting method.
34198036	6	30	theme	broad	1064:1068	arg1	application					1070:1080	a broad application	1062:1080	a broad application	1062:1080	Overall, the fabricated KC/KGM/TiO2 nano-composite films have a broad application in the field of food preservation and packaging applications.
34198036	4	31	theme	reactive	809:816	arg1	species					825:831	reactive oxygen species	809:831	reactive oxygen species	809:831	In particular, the film containing 7 wt% of TiO2 NPs exhibited effective photocatalytic anti-fungal activity (79%) for Penicillium viridicatum after irradiating 6 h through generating reactive oxygen species thus destroying the fungi.
34198036	1	32	theme	konjac	237:242	arg1	KGM					257:259	KGM	257:259	KGM	257:259	A nano-composite film was prepared from k-carrageenan (KC), konjac glucomannan (KGM) and TiO2 nanoparticles (NPs) by solvent casting method.
34198036	1	32	theme	konjac	237:242	arg1	glucomannan					244:254	konjac glucomannan	237:254	konjac glucomannan (KGM)	237:260	A nano-composite film was prepared from k-carrageenan (KC), konjac glucomannan (KGM) and TiO2 nanoparticles (NPs) by solvent casting method.
34198036	0	33	theme	k-carrageenan/konjac	36:55	arg1	film					88:91	k-carrageenan/konjac glucomannan/TiO2 nanocomposite film	36:91	k-carrageenan/konjac glucomannan/TiO2 nanocomposite film with efficient anti-fungal activity	36:127	Preparation and characterization of k-carrageenan/konjac glucomannan/TiO2 nanocomposite film with efficient anti-fungal activity and its application in strawberry preservation.
34198036	3	34	theme	barrier	581:587	arg1	properties					589:598	the mechanical, thermal, structural, and barrier properties	540:598	properties	589:598	The addition of TiO2 NPs to KC/KGM films improved the mechanical, thermal, structural, and barrier properties of nano-composite films.
34198036	0	35	theme	film	88:91	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of k-carrageenan/konjac glucomannan/TiO2 nanocomposite film with efficient anti-fungal activity and its application in strawberry preservation.
34198036	0	35	theme	film	88:91	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of k-carrageenan/konjac glucomannan/TiO2 nanocomposite film with efficient anti-fungal activity and its application in strawberry preservation.
34198036	0	35	theme	film	88:91	arg1	application					137:147	its application	133:147	its application in strawberry preservation	133:174	Preparation and characterization of k-carrageenan/konjac glucomannan/TiO2 nanocomposite film with efficient anti-fungal activity and its application in strawberry preservation.
34198036	5	36	theme	improved	881:888	arg1	characteristics					890:904	the improved characteristics	877:904	the improved characteristics investigated above	877:923	Benefitting from the improved characteristics investigated above, the nano-composite films showed a favorable effect on strawberry storage.
34198036	6	37	theme	applications	1130:1141	arg1	field					1089:1093	the field	1085:1093	the field of food preservation and packaging applications	1085:1141	Overall, the fabricated KC/KGM/TiO2 nano-composite films have a broad application in the field of food preservation and packaging applications.
34198036	4	38	theme	effective	688:696	arg1	activity					725:732	effective photocatalytic anti-fungal activity	688:732	effective photocatalytic anti-fungal activity (79%) for Penicillium viridicatum	688:766	In particular, the film containing 7 wt% of TiO2 NPs exhibited effective photocatalytic anti-fungal activity (79%) for Penicillium viridicatum after irradiating 6 h through generating reactive oxygen species thus destroying the fungi.
34198036	4	38	theme	effective	688:696	arg1	%					737:737	79%	735:737	79%	735:737	In particular, the film containing 7 wt% of TiO2 NPs exhibited effective photocatalytic anti-fungal activity (79%) for Penicillium viridicatum after irradiating 6 h through generating reactive oxygen species thus destroying the fungi.
34198036	0	39	theme	nanocomposite	74:86	arg1	film					88:91	k-carrageenan/konjac glucomannan/TiO2 nanocomposite film	36:91	k-carrageenan/konjac glucomannan/TiO2 nanocomposite film with efficient anti-fungal activity	36:127	Preparation and characterization of k-carrageenan/konjac glucomannan/TiO2 nanocomposite film with efficient anti-fungal activity and its application in strawberry preservation.
34198036	4	40	theme	oxygen	818:823	arg1	species					825:831	reactive oxygen species	809:831	reactive oxygen species	809:831	In particular, the film containing 7 wt% of TiO2 NPs exhibited effective photocatalytic anti-fungal activity (79%) for Penicillium viridicatum after irradiating 6 h through generating reactive oxygen species thus destroying the fungi.
34198036	0	41	from	application	137:147	arg1	preservation					163:174	strawberry preservation	152:174	strawberry preservation	152:174	Preparation and characterization of k-carrageenan/konjac glucomannan/TiO2 nanocomposite film with efficient anti-fungal activity and its application in strawberry preservation.
34198036	3	42	theme	nano-composite	603:616	arg1	films					618:622	nano-composite films	603:622	nano-composite films	603:622	The addition of TiO2 NPs to KC/KGM films improved the mechanical, thermal, structural, and barrier properties of nano-composite films.
34198036	2	43	dep	morphology	322:331	arg1	The					318:320	The	318:320	The	318:320	The morphology and structure of the KC/KGM based nano-composite films prepared from different weight ratio of TiO2 NPs were characterized by SEM, XRD and FT-IR techniques.
34198036	5	44	from	effect	970:975	arg1	storage					991:997	strawberry storage	980:997	strawberry storage	980:997	Benefitting from the improved characteristics investigated above, the nano-composite films showed a favorable effect on strawberry storage.
34198036	3	45	theme	films	618:622	arg1	mechanical					544:553	mechanical	544:553	mechanical	544:553	The addition of TiO2 NPs to KC/KGM films improved the mechanical, thermal, structural, and barrier properties of nano-composite films.
34198036	0	46	with	film	88:91	arg1	activity					120:127	efficient anti-fungal activity	98:127	efficient anti-fungal activity	98:127	Preparation and characterization of k-carrageenan/konjac glucomannan/TiO2 nanocomposite film with efficient anti-fungal activity and its application in strawberry preservation.
34198036	4	47	theme	photocatalytic	698:711	arg1	activity					725:732	effective photocatalytic anti-fungal activity	688:732	effective photocatalytic anti-fungal activity (79%) for Penicillium viridicatum	688:766	In particular, the film containing 7 wt% of TiO2 NPs exhibited effective photocatalytic anti-fungal activity (79%) for Penicillium viridicatum after irradiating 6 h through generating reactive oxygen species thus destroying the fungi.
34198036	4	47	theme	photocatalytic	698:711	arg1	%					737:737	79%	735:737	79%	735:737	In particular, the film containing 7 wt% of TiO2 NPs exhibited effective photocatalytic anti-fungal activity (79%) for Penicillium viridicatum after irradiating 6 h through generating reactive oxygen species thus destroying the fungi.
34198036	4	48	theme	anti-fungal	713:723	arg1	activity					725:732	effective photocatalytic anti-fungal activity	688:732	effective photocatalytic anti-fungal activity (79%) for Penicillium viridicatum	688:766	In particular, the film containing 7 wt% of TiO2 NPs exhibited effective photocatalytic anti-fungal activity (79%) for Penicillium viridicatum after irradiating 6 h through generating reactive oxygen species thus destroying the fungi.
34198036	4	48	theme	anti-fungal	713:723	arg1	%					737:737	79%	735:737	79%	735:737	In particular, the film containing 7 wt% of TiO2 NPs exhibited effective photocatalytic anti-fungal activity (79%) for Penicillium viridicatum after irradiating 6 h through generating reactive oxygen species thus destroying the fungi.
34198036	0	49	theme	efficient	98:106	arg1	activity					120:127	efficient anti-fungal activity	98:127	efficient anti-fungal activity	98:127	Preparation and characterization of k-carrageenan/konjac glucomannan/TiO2 nanocomposite film with efficient anti-fungal activity and its application in strawberry preservation.
34198036	6	50	theme	packaging	1120:1128	arg1	applications					1130:1141	food preservation and packaging applications	1098:1141	food preservation and packaging applications	1098:1141	Overall, the fabricated KC/KGM/TiO2 nano-composite films have a broad application in the field of food preservation and packaging applications.
34198036	3	51	dep	mechanical	544:553	arg1	structural					565:574	structural	565:574	structural	565:574	The addition of TiO2 NPs to KC/KGM films improved the mechanical, thermal, structural, and barrier properties of nano-composite films.
34198036	3	51	dep	mechanical	544:553	arg1	thermal					556:562	thermal	556:562	thermal	556:562	The addition of TiO2 NPs to KC/KGM films improved the mechanical, thermal, structural, and barrier properties of nano-composite films.
34198036	1	52	theme	nano-composite	179:192	arg1	film					194:197	A nano-composite film	177:197	A nano-composite film	177:197	A nano-composite film was prepared from k-carrageenan (KC), konjac glucomannan (KGM) and TiO2 nanoparticles (NPs) by solvent casting method.
34198036	2	53	theme	different	402:410	arg1	ratio					419:423	different weight ratio	402:423	different weight ratio of TiO2 NPs	402:435	The morphology and structure of the KC/KGM based nano-composite films prepared from different weight ratio of TiO2 NPs were characterized by SEM, XRD and FT-IR techniques.
34198036	6	54	theme	nano-composite	1036:1049	arg1	films					1051:1055	the fabricated KC/KGM/TiO2 nano-composite films	1009:1055	the fabricated KC/KGM/TiO2 nano-composite films	1009:1055	Overall, the fabricated KC/KGM/TiO2 nano-composite films have a broad application in the field of food preservation and packaging applications.
34198036	2	55	theme	nano-composite	367:380	arg1	films					382:386	the KC/KGM based nano-composite films	350:386	the KC/KGM based nano-composite films prepared from different weight ratio of TiO2 NPs	350:435	The morphology and structure of the KC/KGM based nano-composite films prepared from different weight ratio of TiO2 NPs were characterized by SEM, XRD and FT-IR techniques.
34198036	6	56	theme	KC/KGM/TiO2	1024:1034	arg1	films					1051:1055	the fabricated KC/KGM/TiO2 nano-composite films	1009:1055	the fabricated KC/KGM/TiO2 nano-composite films	1009:1055	Overall, the fabricated KC/KGM/TiO2 nano-composite films have a broad application in the field of food preservation and packaging applications.
33389169	8	0	dep	alternative	1509:1519	arg1	compared					1524:1531	compared	1524:1531	compared to commercial petroleum-derived compounds	1524:1573	The submerged fermentation demonstrates the huge potential of Phoma dimorpha to produce polysaccharides with bioemulsifying properties as a biotechnologically cleaner alternative if compared to commercial petroleum-derived compounds.
33389169	4	1	theme	best	687:690	arg1	condition					703:711	The best extraction condition	683:711	The best extraction condition of PEF	683:718	The best extraction condition of PEF yielded 12.02 wt% and was achieved when using UAE with direct sonication for 30 min under the intensity of 75.11 W/cm2 and pulse factor of 0.57.
33389169	5	2	theme	kinetic	872:878	arg1	profiles					880:887	the kinetic profiles	868:887	the kinetic profiles	868:887	In the kinetic profiles, the highest yield (15.28 wt%) was obtained at 50 °C under an ultrasound intensity of 75.11 W/cm2 and a pulse factor of 0.93.
33389169	1	3	theme	dimorpha	263:270	arg1	biomass					281:287	Phoma dimorpha mycelial biomass	257:287	Phoma dimorpha mycelial biomass	257:287	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	8	4	theme	cleaner	1501:1507	arg1	polysaccharides					1430:1444	polysaccharides	1430:1444	polysaccharides with bioemulsifying properties	1430:1475	The submerged fermentation demonstrates the huge potential of Phoma dimorpha to produce polysaccharides with bioemulsifying properties as a biotechnologically cleaner alternative if compared to commercial petroleum-derived compounds.
33389169	8	4	theme	cleaner	1501:1507	arg1	alternative					1509:1519	a biotechnologically cleaner alternative	1480:1519	a biotechnologically cleaner alternative if compared to commercial petroleum-derived compounds	1480:1573	The submerged fermentation demonstrates the huge potential of Phoma dimorpha to produce polysaccharides with bioemulsifying properties as a biotechnologically cleaner alternative if compared to commercial petroleum-derived compounds.
33389169	4	5	theme	factor	849:854	arg1	intensity					814:822	the intensity	810:822	the intensity of 75.11 W/cm2 and pulse factor of 0.57	810:862	The best extraction condition of PEF yielded 12.02 wt% and was achieved when using UAE with direct sonication for 30 min under the intensity of 75.11 W/cm2 and pulse factor of 0.57.
33389169	4	6	theme	extraction	692:701	arg1	condition					703:711	The best extraction condition	683:711	The best extraction condition of PEF	683:718	The best extraction condition of PEF yielded 12.02 wt% and was achieved when using UAE with direct sonication for 30 min under the intensity of 75.11 W/cm2 and pulse factor of 0.57.
33389169	1	7	theme	mycelial	272:279	arg1	biomass					281:287	Phoma dimorpha mycelial biomass	257:287	Phoma dimorpha mycelial biomass	257:287	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	6	8	theme	extracted	1038:1046	arg1	polysaccharide					1048:1061	extracted polysaccharide	1038:1061	extracted polysaccharide	1038:1061	Structural analysis of extracted polysaccharide was performed using Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and thermal property.
33389169	6	9	theme	thermal	1177:1183	arg1	property					1185:1192	thermal property	1177:1192	thermal property	1177:1192	Structural analysis of extracted polysaccharide was performed using Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and thermal property.
33389169	8	10	with	polysaccharides	1430:1444	arg1	properties					1466:1475	bioemulsifying properties	1451:1475	bioemulsifying properties	1451:1475	The submerged fermentation demonstrates the huge potential of Phoma dimorpha to produce polysaccharides with bioemulsifying properties as a biotechnologically cleaner alternative if compared to commercial petroleum-derived compounds.
33389169	8	11	theme	huge	1386:1389	arg1	potential					1391:1399	the huge potential	1382:1399	the huge potential of Phoma dimorpha to produce polysaccharides with bioemulsifying properties as a biotechnologically cleaner alternative if compared to commercial petroleum-derived compounds	1382:1573	The submerged fermentation demonstrates the huge potential of Phoma dimorpha to produce polysaccharides with bioemulsifying properties as a biotechnologically cleaner alternative if compared to commercial petroleum-derived compounds.
33389169	2	12	from	extraction	334:343	arg1	terms					376:380	terms	376:380	terms of polysaccharides-enriched fractions (PEF) yield	376:430	These methods were compared to conventional extraction (hot water extraction, HWE) in terms of polysaccharides-enriched fractions (PEF) yield.
33389169	3	13	with	selection	595:603	arg1	yield					639:643	the highest yield	627:643	the highest yield using water as an extraction solvent	627:680	A central composite rotational design was performed for each extraction method to investigate the influence of independent variables on the yield and to help the selection of the condition with the highest yield using water as an extraction solvent.
33389169	4	14	theme	0.57	859:862	arg1	75.11 W/cm2					827:837	75.11 W/cm2	827:837	75.11 W/cm2	827:837	The best extraction condition of PEF yielded 12.02 wt% and was achieved when using UAE with direct sonication for 30 min under the intensity of 75.11 W/cm2 and pulse factor of 0.57.
33389169	4	14	theme	0.57	859:862	arg1	factor					849:854	pulse factor	843:854	pulse factor	843:854	The best extraction condition of PEF yielded 12.02 wt% and was achieved when using UAE with direct sonication for 30 min under the intensity of 75.11 W/cm2 and pulse factor of 0.57.
33389169	3	15	from	influence	531:539	arg1	yield					573:577	the yield	569:577	the yield	569:577	A central composite rotational design was performed for each extraction method to investigate the influence of independent variables on the yield and to help the selection of the condition with the highest yield using water as an extraction solvent.
33389169	4	16	theme	12.02	728:732	arg1	%					736:736	12.02 wt%	728:736	12.02 wt%	728:736	The best extraction condition of PEF yielded 12.02 wt% and was achieved when using UAE with direct sonication for 30 min under the intensity of 75.11 W/cm2 and pulse factor of 0.57.
33389169	1	17	theme	hot	160:162	arg1	extraction					170:179	pressurized hot water extraction	148:179	pressurized hot water extraction (PHWE)	148:186	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	1	17	theme	hot	160:162	arg1	methods					218:224	advanced clean methods	203:224	advanced clean methods	203:224	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	1	17	theme	hot	160:162	arg1	extraction					127:136	Ultrasound-assisted extraction	107:136	Ultrasound-assisted extraction (UAE)	107:142	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	1	17	theme	hot	160:162	arg1	PHWE					182:185	PHWE	182:185	PHWE	182:185	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	6	18	theme	infrared	1101:1108	arg1	spectroscopy					1110:1121	Fourier-transform infrared spectroscopy	1083:1121	Fourier-transform infrared spectroscopy	1083:1121	Structural analysis of extracted polysaccharide was performed using Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and thermal property.
33389169	4	19	theme	75.11 W/cm2	827:837	arg1	intensity					814:822	the intensity	810:822	the intensity of 75.11 W/cm2 and pulse factor of 0.57	810:862	The best extraction condition of PEF yielded 12.02 wt% and was achieved when using UAE with direct sonication for 30 min under the intensity of 75.11 W/cm2 and pulse factor of 0.57.
33389169	1	20	theme	clean	212:216	arg1	extraction					127:136	Ultrasound-assisted extraction	107:136	Ultrasound-assisted extraction (UAE)	107:142	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	1	20	theme	clean	212:216	arg1	methods					218:224	advanced clean methods	203:224	advanced clean methods	203:224	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	1	20	theme	clean	212:216	arg1	extraction					170:179	pressurized hot water extraction	148:179	pressurized hot water extraction (PHWE)	148:186	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	3	21	theme	independent	544:554	arg1	variables					556:564	independent variables	544:564	independent variables	544:564	A central composite rotational design was performed for each extraction method to investigate the influence of independent variables on the yield and to help the selection of the condition with the highest yield using water as an extraction solvent.
33389169	5	22	theme	highest	894:900	arg1	%					917:917	15.28 wt%	909:917	15.28 wt%	909:917	In the kinetic profiles, the highest yield (15.28 wt%) was obtained at 50 °C under an ultrasound intensity of 75.11 W/cm2 and a pulse factor of 0.93.
33389169	5	22	theme	highest	894:900	arg1	yield					902:906	the highest yield	890:906	the highest yield (15.28 wt%)	890:918	In the kinetic profiles, the highest yield (15.28 wt%) was obtained at 50 °C under an ultrasound intensity of 75.11 W/cm2 and a pulse factor of 0.93.
33389169	3	23	theme	composite	443:451	arg1	design					464:469	A central composite rotational design	433:469	A central composite rotational design	433:469	A central composite rotational design was performed for each extraction method to investigate the influence of independent variables on the yield and to help the selection of the condition with the highest yield using water as an extraction solvent.
33389169	3	24	theme	variables	556:564	arg1	influence					531:539	the influence	527:539	the influence of independent variables on the yield	527:577	A central composite rotational design was performed for each extraction method to investigate the influence of independent variables on the yield and to help the selection of the condition with the highest yield using water as an extraction solvent.
33389169	8	25	theme	petroleum-derived	1547:1563	arg1	compounds					1565:1573	commercial petroleum-derived compounds	1536:1573	commercial petroleum-derived compounds	1536:1573	The submerged fermentation demonstrates the huge potential of Phoma dimorpha to produce polysaccharides with bioemulsifying properties as a biotechnologically cleaner alternative if compared to commercial petroleum-derived compounds.
33389169	2	26	theme	fractions	410:418	arg1	yield					426:430	polysaccharides-enriched fractions (PEF) yield	385:430	polysaccharides-enriched fractions (PEF) yield	385:430	These methods were compared to conventional extraction (hot water extraction, HWE) in terms of polysaccharides-enriched fractions (PEF) yield.
33389169	4	27	theme	pulse	843:847	arg1	factor					849:854	pulse factor	843:854	pulse factor	843:854	The best extraction condition of PEF yielded 12.02 wt% and was achieved when using UAE with direct sonication for 30 min under the intensity of 75.11 W/cm2 and pulse factor of 0.57.
33389169	7	28	theme	water	1199:1203	arg1	%					1294:1294	31.3 ± 1.5%	1284:1294	31.3 ± 1.5%	1284:1294	The water solubility index, water holding capacity, and emulsification index of PEF were 31.3 ± 1.5%, 138.1 ± 3.2%, and 62.9 ± 2.3%, respectively.
33389169	7	28	theme	water	1199:1203	arg1	index					1216:1220	The water solubility index	1195:1220	The water solubility index	1195:1220	The water solubility index, water holding capacity, and emulsification index of PEF were 31.3 ± 1.5%, 138.1 ± 3.2%, and 62.9 ± 2.3%, respectively.
33389169	1	29	theme	Ultrasound-assisted	107:125	arg1	extraction					127:136	Ultrasound-assisted extraction	107:136	Ultrasound-assisted extraction (UAE)	107:142	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	1	29	theme	Ultrasound-assisted	107:125	arg1	extraction					170:179	pressurized hot water extraction	148:179	pressurized hot water extraction (PHWE)	148:186	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	1	29	theme	Ultrasound-assisted	107:125	arg1	methods					218:224	advanced clean methods	203:224	advanced clean methods	203:224	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	1	29	theme	Ultrasound-assisted	107:125	arg1	UAE					139:141	UAE	139:141	UAE	139:141	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	9	30	theme	PEF	1675:1677	arg1	extraction					1679:1688	PEF extraction	1675:1688	PEF extraction	1675:1688	Furthermore, UAE and PHWE are green technologies, which can be operated at an industrial scale for PEF extraction.
33389169	2	31	theme	polysaccharides-enriched	385:408	arg1	fractions					410:418	polysaccharides-enriched fractions	385:418	polysaccharides-enriched fractions (PEF) yield	385:430	These methods were compared to conventional extraction (hot water extraction, HWE) in terms of polysaccharides-enriched fractions (PEF) yield.
33389169	2	31	theme	polysaccharides-enriched	385:408	arg1	PEF					421:423	PEF	421:423	PEF	421:423	These methods were compared to conventional extraction (hot water extraction, HWE) in terms of polysaccharides-enriched fractions (PEF) yield.
33389169	4	32	with	UAE	766:768	arg1	sonication					782:791	direct sonication	775:791	direct sonication	775:791	The best extraction condition of PEF yielded 12.02 wt% and was achieved when using UAE with direct sonication for 30 min under the intensity of 75.11 W/cm2 and pulse factor of 0.57.
33389169	8	33	theme	bioemulsifying	1451:1464	arg1	properties					1466:1475	bioemulsifying properties	1451:1475	bioemulsifying properties	1451:1475	The submerged fermentation demonstrates the huge potential of Phoma dimorpha to produce polysaccharides with bioemulsifying properties as a biotechnologically cleaner alternative if compared to commercial petroleum-derived compounds.
33389169	4	34	theme	wt	734:735	arg1	%					736:736	12.02 wt%	728:736	12.02 wt%	728:736	The best extraction condition of PEF yielded 12.02 wt% and was achieved when using UAE with direct sonication for 30 min under the intensity of 75.11 W/cm2 and pulse factor of 0.57.
33389169	3	35	theme	central	435:441	arg1	design					464:469	A central composite rotational design	433:469	A central composite rotational design	433:469	A central composite rotational design was performed for each extraction method to investigate the influence of independent variables on the yield and to help the selection of the condition with the highest yield using water as an extraction solvent.
33389169	6	36	theme	Structural	1015:1024	arg1	analysis					1026:1033	Structural analysis	1015:1033	Structural analysis of extracted polysaccharide	1015:1061	Structural analysis of extracted polysaccharide was performed using Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and thermal property.
33389169	1	37	from	biomass	281:287	arg1	polysaccharides					236:250	polysaccharides	236:250	polysaccharides from Phoma dimorpha mycelial biomass	236:287	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	7	38	theme	water	1223:1227	arg1	capacity					1237:1244	water holding capacity	1223:1244	water holding capacity	1223:1244	The water solubility index, water holding capacity, and emulsification index of PEF were 31.3 ± 1.5%, 138.1 ± 3.2%, and 62.9 ± 2.3%, respectively.
33389169	2	39	theme	conventional	321:332	arg1	extraction					334:343	conventional extraction	321:343	conventional extraction (hot water extraction, HWE) in terms of polysaccharides-enriched fractions (PEF) yield	321:430	These methods were compared to conventional extraction (hot water extraction, HWE) in terms of polysaccharides-enriched fractions (PEF) yield.
33389169	1	40	theme	advanced	203:210	arg1	extraction					127:136	Ultrasound-assisted extraction	107:136	Ultrasound-assisted extraction (UAE)	107:142	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	1	40	theme	advanced	203:210	arg1	methods					218:224	advanced clean methods	203:224	advanced clean methods	203:224	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	1	40	theme	advanced	203:210	arg1	extraction					170:179	pressurized hot water extraction	148:179	pressurized hot water extraction (PHWE)	148:186	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	6	41	theme	X-ray	1124:1128	arg1	diffraction					1130:1140	X-ray diffraction	1124:1140	X-ray diffraction	1124:1140	Structural analysis of extracted polysaccharide was performed using Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and thermal property.
33389169	3	42	theme	rotational	453:462	arg1	design					464:469	A central composite rotational design	433:469	A central composite rotational design	433:469	A central composite rotational design was performed for each extraction method to investigate the influence of independent variables on the yield and to help the selection of the condition with the highest yield using water as an extraction solvent.
33389169	6	43	theme	scanning	1143:1150	arg1	microscopy					1161:1170	scanning electron microscopy	1143:1170	scanning electron microscopy	1143:1170	Structural analysis of extracted polysaccharide was performed using Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and thermal property.
33389169	5	44	theme	0.93	1009:1012	arg1	factor					999:1004	a pulse factor	991:1004	a pulse factor of 0.93	991:1012	In the kinetic profiles, the highest yield (15.28 wt%) was obtained at 50 °C under an ultrasound intensity of 75.11 W/cm2 and a pulse factor of 0.93.
33389169	5	44	theme	0.93	1009:1012	arg1	intensity					962:970	an ultrasound intensity	948:970	an ultrasound intensity of 75.11 W/cm2	948:985	In the kinetic profiles, the highest yield (15.28 wt%) was obtained at 50 °C under an ultrasound intensity of 75.11 W/cm2 and a pulse factor of 0.93.
33389169	2	45	dep	extraction	334:343	arg1	extraction					356:365	hot water extraction	346:365	hot water extraction	346:365	These methods were compared to conventional extraction (hot water extraction, HWE) in terms of polysaccharides-enriched fractions (PEF) yield.
33389169	2	45	dep	extraction	334:343	arg1	HWE					368:370	HWE	368:370	HWE	368:370	These methods were compared to conventional extraction (hot water extraction, HWE) in terms of polysaccharides-enriched fractions (PEF) yield.
33389169	2	46	theme	yield	426:430	arg1	terms					376:380	terms	376:380	terms of polysaccharides-enriched fractions (PEF) yield	376:430	These methods were compared to conventional extraction (hot water extraction, HWE) in terms of polysaccharides-enriched fractions (PEF) yield.
33389169	0	47	theme	fractions	59:67	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Extraction and characterization of polysaccharide-enriched fractions from Phoma dimorpha mycelial biomass.
33389169	0	47	theme	fractions	59:67	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and characterization of polysaccharide-enriched fractions from Phoma dimorpha mycelial biomass.
33389169	7	48	theme	emulsification	1251:1264	arg1	index					1266:1270	emulsification index	1251:1270	emulsification index of PEF	1251:1277	The water solubility index, water holding capacity, and emulsification index of PEF were 31.3 ± 1.5%, 138.1 ± 3.2%, and 62.9 ± 2.3%, respectively.
33389169	0	49	from	biomass	98:104	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Extraction and characterization of polysaccharide-enriched fractions from Phoma dimorpha mycelial biomass.
33389169	0	49	from	biomass	98:104	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and characterization of polysaccharide-enriched fractions from Phoma dimorpha mycelial biomass.
33389169	8	50	theme	submerged	1346:1354	arg1	fermentation					1356:1367	The submerged fermentation	1342:1367	The submerged fermentation	1342:1367	The submerged fermentation demonstrates the huge potential of Phoma dimorpha to produce polysaccharides with bioemulsifying properties as a biotechnologically cleaner alternative if compared to commercial petroleum-derived compounds.
33389169	8	51	theme	commercial	1536:1545	arg1	compounds					1565:1573	commercial petroleum-derived compounds	1536:1573	commercial petroleum-derived compounds	1536:1573	The submerged fermentation demonstrates the huge potential of Phoma dimorpha to produce polysaccharides with bioemulsifying properties as a biotechnologically cleaner alternative if compared to commercial petroleum-derived compounds.
33389169	0	52	theme	polysaccharide-enriched	35:57	arg1	fractions					59:67	polysaccharide-enriched fractions	35:67	polysaccharide-enriched fractions	35:67	Extraction and characterization of polysaccharide-enriched fractions from Phoma dimorpha mycelial biomass.
33389169	8	53	link	petroleum-derived	1547:1563	arg1	compounds					1565:1573	commercial petroleum-derived compounds	1536:1573	commercial petroleum-derived compounds	1536:1573	The submerged fermentation demonstrates the huge potential of Phoma dimorpha to produce polysaccharides with bioemulsifying properties as a biotechnologically cleaner alternative if compared to commercial petroleum-derived compounds.
33389169	0	54	theme	Phoma	74:78	arg1	biomass					98:104	Phoma dimorpha mycelial biomass	74:104	Phoma dimorpha mycelial biomass	74:104	Extraction and characterization of polysaccharide-enriched fractions from Phoma dimorpha mycelial biomass.
33389169	7	55	theme	solubility	1205:1214	arg1	%					1294:1294	31.3 ± 1.5%	1284:1294	31.3 ± 1.5%	1284:1294	The water solubility index, water holding capacity, and emulsification index of PEF were 31.3 ± 1.5%, 138.1 ± 3.2%, and 62.9 ± 2.3%, respectively.
33389169	7	55	theme	solubility	1205:1214	arg1	index					1216:1220	The water solubility index	1195:1220	The water solubility index	1195:1220	The water solubility index, water holding capacity, and emulsification index of PEF were 31.3 ± 1.5%, 138.1 ± 3.2%, and 62.9 ± 2.3%, respectively.
33389169	5	56	theme	75.11 W/cm2	975:985	arg1	factor					999:1004	a pulse factor	991:1004	a pulse factor of 0.93	991:1012	In the kinetic profiles, the highest yield (15.28 wt%) was obtained at 50 °C under an ultrasound intensity of 75.11 W/cm2 and a pulse factor of 0.93.
33389169	5	56	theme	75.11 W/cm2	975:985	arg1	intensity					962:970	an ultrasound intensity	948:970	an ultrasound intensity of 75.11 W/cm2	948:985	In the kinetic profiles, the highest yield (15.28 wt%) was obtained at 50 °C under an ultrasound intensity of 75.11 W/cm2 and a pulse factor of 0.93.
33389169	4	57	theme	PEF	716:718	arg1	condition					703:711	The best extraction condition	683:711	The best extraction condition of PEF	683:718	The best extraction condition of PEF yielded 12.02 wt% and was achieved when using UAE with direct sonication for 30 min under the intensity of 75.11 W/cm2 and pulse factor of 0.57.
33389169	6	58	theme	electron	1152:1159	arg1	microscopy					1161:1170	scanning electron microscopy	1143:1170	scanning electron microscopy	1143:1170	Structural analysis of extracted polysaccharide was performed using Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and thermal property.
33389169	9	59	theme	industrial	1654:1663	arg1	scale					1665:1669	an industrial scale	1651:1669	an industrial scale for PEF extraction	1651:1688	Furthermore, UAE and PHWE are green technologies, which can be operated at an industrial scale for PEF extraction.
33389169	6	60	theme	Fourier-transform	1083:1099	arg1	spectroscopy					1110:1121	Fourier-transform infrared spectroscopy	1083:1121	Fourier-transform infrared spectroscopy	1083:1121	Structural analysis of extracted polysaccharide was performed using Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and thermal property.
33389169	8	61	theme	Phoma	1404:1408	arg1	dimorpha					1410:1417	Phoma dimorpha	1404:1417	Phoma dimorpha	1404:1417	The submerged fermentation demonstrates the huge potential of Phoma dimorpha to produce polysaccharides with bioemulsifying properties as a biotechnologically cleaner alternative if compared to commercial petroleum-derived compounds.
33389169	7	62	theme	holding	1229:1235	arg1	capacity					1237:1244	water holding capacity	1223:1244	water holding capacity	1223:1244	The water solubility index, water holding capacity, and emulsification index of PEF were 31.3 ± 1.5%, 138.1 ± 3.2%, and 62.9 ± 2.3%, respectively.
33389169	9	63	theme	green	1606:1610	arg1	PHWE					1597:1600	PHWE	1597:1600	PHWE	1597:1600	Furthermore, UAE and PHWE are green technologies, which can be operated at an industrial scale for PEF extraction.
33389169	9	63	theme	green	1606:1610	arg1	technologies					1612:1623	green technologies	1606:1623	green technologies	1606:1623	Furthermore, UAE and PHWE are green technologies, which can be operated at an industrial scale for PEF extraction.
33389169	9	63	theme	green	1606:1610	arg1	UAE					1589:1591	UAE	1589:1591	UAE	1589:1591	Furthermore, UAE and PHWE are green technologies, which can be operated at an industrial scale for PEF extraction.
33389169	5	64	theme	pulse	993:997	arg1	factor					999:1004	a pulse factor	991:1004	a pulse factor of 0.93	991:1012	In the kinetic profiles, the highest yield (15.28 wt%) was obtained at 50 °C under an ultrasound intensity of 75.11 W/cm2 and a pulse factor of 0.93.
33389169	4	65	theme	direct	775:780	arg1	sonication					782:791	direct sonication	775:791	direct sonication	775:791	The best extraction condition of PEF yielded 12.02 wt% and was achieved when using UAE with direct sonication for 30 min under the intensity of 75.11 W/cm2 and pulse factor of 0.57.
33389169	1	66	theme	pressurized	148:158	arg1	extraction					170:179	pressurized hot water extraction	148:179	pressurized hot water extraction (PHWE)	148:186	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	1	66	theme	pressurized	148:158	arg1	methods					218:224	advanced clean methods	203:224	advanced clean methods	203:224	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	1	66	theme	pressurized	148:158	arg1	extraction					127:136	Ultrasound-assisted extraction	107:136	Ultrasound-assisted extraction (UAE)	107:142	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	1	66	theme	pressurized	148:158	arg1	PHWE					182:185	PHWE	182:185	PHWE	182:185	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	5	67	theme	wt	915:916	arg1	%					917:917	15.28 wt%	909:917	15.28 wt%	909:917	In the kinetic profiles, the highest yield (15.28 wt%) was obtained at 50 °C under an ultrasound intensity of 75.11 W/cm2 and a pulse factor of 0.93.
33389169	5	67	theme	wt	915:916	arg1	yield					902:906	the highest yield	890:906	the highest yield (15.28 wt%)	890:918	In the kinetic profiles, the highest yield (15.28 wt%) was obtained at 50 °C under an ultrasound intensity of 75.11 W/cm2 and a pulse factor of 0.93.
33389169	8	68	theme	dimorpha	1410:1417	arg1	potential					1391:1399	the huge potential	1382:1399	the huge potential of Phoma dimorpha to produce polysaccharides with bioemulsifying properties as a biotechnologically cleaner alternative if compared to commercial petroleum-derived compounds	1382:1573	The submerged fermentation demonstrates the huge potential of Phoma dimorpha to produce polysaccharides with bioemulsifying properties as a biotechnologically cleaner alternative if compared to commercial petroleum-derived compounds.
33389169	3	69	theme	highest	631:637	arg1	yield					639:643	the highest yield	627:643	the highest yield using water as an extraction solvent	627:680	A central composite rotational design was performed for each extraction method to investigate the influence of independent variables on the yield and to help the selection of the condition with the highest yield using water as an extraction solvent.
33389169	2	70	theme	hot	346:348	arg1	extraction					356:365	hot water extraction	346:365	hot water extraction	346:365	These methods were compared to conventional extraction (hot water extraction, HWE) in terms of polysaccharides-enriched fractions (PEF) yield.
33389169	2	70	theme	hot	346:348	arg1	HWE					368:370	HWE	368:370	HWE	368:370	These methods were compared to conventional extraction (hot water extraction, HWE) in terms of polysaccharides-enriched fractions (PEF) yield.
33389169	2	71	theme	water	350:354	arg1	extraction					356:365	hot water extraction	346:365	hot water extraction	346:365	These methods were compared to conventional extraction (hot water extraction, HWE) in terms of polysaccharides-enriched fractions (PEF) yield.
33389169	2	71	theme	water	350:354	arg1	HWE					368:370	HWE	368:370	HWE	368:370	These methods were compared to conventional extraction (hot water extraction, HWE) in terms of polysaccharides-enriched fractions (PEF) yield.
33389169	0	72	theme	mycelial	89:96	arg1	biomass					98:104	Phoma dimorpha mycelial biomass	74:104	Phoma dimorpha mycelial biomass	74:104	Extraction and characterization of polysaccharide-enriched fractions from Phoma dimorpha mycelial biomass.
33389169	3	73	theme	extraction	663:672	arg1	water					651:655	water	651:655	water	651:655	A central composite rotational design was performed for each extraction method to investigate the influence of independent variables on the yield and to help the selection of the condition with the highest yield using water as an extraction solvent.
33389169	3	73	theme	extraction	663:672	arg1	solvent					674:680	an extraction solvent	660:680	an extraction solvent	660:680	A central composite rotational design was performed for each extraction method to investigate the influence of independent variables on the yield and to help the selection of the condition with the highest yield using water as an extraction solvent.
33389169	3	74	theme	condition	612:620	arg1	selection					595:603	the selection	591:603	the selection of the condition with the highest yield using water as an extraction solvent	591:680	A central composite rotational design was performed for each extraction method to investigate the influence of independent variables on the yield and to help the selection of the condition with the highest yield using water as an extraction solvent.
33389169	7	75	theme	PEF	1275:1277	arg1	%					1294:1294	31.3 ± 1.5%	1284:1294	31.3 ± 1.5%	1284:1294	The water solubility index, water holding capacity, and emulsification index of PEF were 31.3 ± 1.5%, 138.1 ± 3.2%, and 62.9 ± 2.3%, respectively.
33389169	7	75	theme	PEF	1275:1277	arg1	index					1216:1220	The water solubility index	1195:1220	The water solubility index	1195:1220	The water solubility index, water holding capacity, and emulsification index of PEF were 31.3 ± 1.5%, 138.1 ± 3.2%, and 62.9 ± 2.3%, respectively.
33389169	7	75	theme	PEF	1275:1277	arg1	index					1266:1270	emulsification index	1251:1270	emulsification index of PEF	1251:1277	The water solubility index, water holding capacity, and emulsification index of PEF were 31.3 ± 1.5%, 138.1 ± 3.2%, and 62.9 ± 2.3%, respectively.
33389169	7	75	theme	PEF	1275:1277	arg1	capacity					1237:1244	water holding capacity	1223:1244	water holding capacity	1223:1244	The water solubility index, water holding capacity, and emulsification index of PEF were 31.3 ± 1.5%, 138.1 ± 3.2%, and 62.9 ± 2.3%, respectively.
33389169	1	76	theme	water	164:168	arg1	extraction					170:179	pressurized hot water extraction	148:179	pressurized hot water extraction (PHWE)	148:186	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	1	76	theme	water	164:168	arg1	methods					218:224	advanced clean methods	203:224	advanced clean methods	203:224	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	1	76	theme	water	164:168	arg1	extraction					127:136	Ultrasound-assisted extraction	107:136	Ultrasound-assisted extraction (UAE)	107:142	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	1	76	theme	water	164:168	arg1	PHWE					182:185	PHWE	182:185	PHWE	182:185	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
33389169	3	77	theme	extraction	494:503	arg1	method					505:510	each extraction method	489:510	each extraction method	489:510	A central composite rotational design was performed for each extraction method to investigate the influence of independent variables on the yield and to help the selection of the condition with the highest yield using water as an extraction solvent.
33389169	0	78	theme	dimorpha	80:87	arg1	biomass					98:104	Phoma dimorpha mycelial biomass	74:104	Phoma dimorpha mycelial biomass	74:104	Extraction and characterization of polysaccharide-enriched fractions from Phoma dimorpha mycelial biomass.
33389169	5	79	theme	ultrasound	951:960	arg1	intensity					962:970	an ultrasound intensity	948:970	an ultrasound intensity of 75.11 W/cm2	948:985	In the kinetic profiles, the highest yield (15.28 wt%) was obtained at 50 °C under an ultrasound intensity of 75.11 W/cm2 and a pulse factor of 0.93.
33389169	6	80	theme	polysaccharide	1048:1061	arg1	analysis					1026:1033	Structural analysis	1015:1033	Structural analysis of extracted polysaccharide	1015:1061	Structural analysis of extracted polysaccharide was performed using Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and thermal property.
33389169	1	81	theme	Phoma	257:261	arg1	biomass					281:287	Phoma dimorpha mycelial biomass	257:287	Phoma dimorpha mycelial biomass	257:287	Ultrasound-assisted extraction (UAE) and pressurized hot water extraction (PHWE) were tested as advanced clean methods to obtain polysaccharides from Phoma dimorpha mycelial biomass.
34655584	0	0	theme	arabinose-branched	84:101	arg1	EPS-M2					121:126	the highly arabinose-branched exopolysaccharide EPS-M2	73:126	the highly arabinose-branched exopolysaccharide EPS-M2	73:126	Structure, physicochemical characterization, and antioxidant activity of the highly arabinose-branched exopolysaccharide EPS-M2 from Streptococcus thermophilus CS6.
34655584	4	1	theme	-α-L-Araf-	907:916	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	2	theme	}	836:836	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	3	theme	was	740:742	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	4	theme	-β-D-Galp-	942:951	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	5	theme	arabinose	625:633	arg1	5:2.5:1					648:654	5:2.5:1	648:654	5:2.5:1	648:654	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	5	theme	arabinose	625:633	arg1	-β-D-Galp-					1031:1040	galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→	614:1043	-β-D-Galp-	1031:1040	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	6	6	theme	potential	1182:1190	arg1	CS6					1227:1229	a potential functional starter S. thermophilus CS6	1180:1229	a potential functional starter S. thermophilus CS6	1180:1229	Overall, EPS-M2 from a potential functional starter S. thermophilus CS6 provided opportunities for natural thickener, stabilizer, and antioxidant agent exploration in the food industry.
34655584	6	7	dep	S.	1211:1212	arg1	thermophilus					1214:1225	thermophilus	1214:1225	thermophilus	1214:1225	Overall, EPS-M2 from a potential functional starter S. thermophilus CS6 provided opportunities for natural thickener, stabilizer, and antioxidant agent exploration in the food industry.
34655584	4	8	theme	unit	735:738	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	0	9	theme	EPS-M2	121:126	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure, physicochemical characterization, and antioxidant activity of the highly arabinose-branched exopolysaccharide EPS-M2 from Streptococcus thermophilus CS6.
34655584	0	9	theme	EPS-M2	121:126	arg1	characterization					27:42	physicochemical characterization	11:42	physicochemical characterization	11:42	Structure, physicochemical characterization, and antioxidant activity of the highly arabinose-branched exopolysaccharide EPS-M2 from Streptococcus thermophilus CS6.
34655584	0	9	theme	EPS-M2	121:126	arg1	activity					61:68	antioxidant activity	49:68	antioxidant activity	49:68	Structure, physicochemical characterization, and antioxidant activity of the highly arabinose-branched exopolysaccharide EPS-M2 from Streptococcus thermophilus CS6.
34655584	3	10	dep	fractions	414:422	arg1	fractions					414:422	two purified fractions	401:422	two purified fractions (EPS-M1 and EPS-M2)	401:442	In this study, two purified fractions (EPS-M1 and EPS-M2) exhibited good rheology, thermostability and antioxidant activity.
34655584	3	10	dep	fractions	414:422	arg1	EPS-M2					436:441	EPS-M2	436:441	EPS-M2	436:441	In this study, two purified fractions (EPS-M1 and EPS-M2) exhibited good rheology, thermostability and antioxidant activity.
34655584	3	10	dep	fractions	414:422	arg1	EPS-M1					425:430	EPS-M1	425:430	EPS-M1	425:430	In this study, two purified fractions (EPS-M1 and EPS-M2) exhibited good rheology, thermostability and antioxidant activity.
34655584	4	11	theme	1 → 4	777:781	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	12	theme	glucose	639:645	arg1	5:2.5:1					648:654	5:2.5:1	648:654	5:2.5:1	648:654	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	12	theme	glucose	639:645	arg1	-β-D-Galp-					1031:1040	galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→	614:1043	-β-D-Galp-	1031:1040	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	0	13	theme	exopolysaccharide	103:119	arg1	EPS-M2					121:126	the highly arabinose-branched exopolysaccharide EPS-M2	73:126	the highly arabinose-branched exopolysaccharide EPS-M2	73:126	Structure, physicochemical characterization, and antioxidant activity of the highly arabinose-branched exopolysaccharide EPS-M2 from Streptococcus thermophilus CS6.
34655584	4	14	theme	Further	511:517	arg1	composition					534:544	Further monosaccharide composition	511:544	Further monosaccharide composition	511:544	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	15	theme	-α-L-Araf-	837:846	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	16	theme	{	995:995	arg1	α-L-Araf-					996:1004	{α-L-Araf-	995:1004	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	17	theme	-[β-D-Galp-	889:899	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	5	18	theme	sugar	1141:1145	arg1	metabolism					1147:1156	nucleotide sugar metabolism	1130:1156	nucleotide sugar metabolism	1130:1156	High EPS production relied on the expression of eps gene cluster and key enzymes of nucleotide sugar metabolism.
34655584	4	19	theme	α-L-Araf-	820:828	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	20	theme	-β-D-Galp-	783:792	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	5	21	theme	metabolism	1147:1156	arg1	expression					1080:1089	the expression	1076:1089	the expression of eps gene cluster	1076:1109	High EPS production relied on the expression of eps gene cluster and key enzymes of nucleotide sugar metabolism.
34655584	5	21	theme	metabolism	1147:1156	arg1	enzymes					1119:1125	key enzymes	1115:1125	key enzymes of nucleotide sugar metabolism	1115:1156	High EPS production relied on the expression of eps gene cluster and key enzymes of nucleotide sugar metabolism.
34655584	4	22	theme	2.22 × 104 Da	693:705	arg1	weight					683:688	an average molecular weight	662:688	an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	662:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	23	theme	{	819:819	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	5	24	theme	gene	1098:1101	arg1	cluster					1103:1109	eps gene cluster	1094:1109	eps gene cluster	1094:1109	High EPS production relied on the expression of eps gene cluster and key enzymes of nucleotide sugar metabolism.
34655584	1	25	theme	high	214:217	arg1	exopolysaccharide					219:235	high exopolysaccharide	214:235	the high exopolysaccharide (EPS) level in optimized skimmed milk medium	210:280	Streptococcus thermophilus CS6 could produce the high exopolysaccharide (EPS) level in optimized skimmed milk medium.
34655584	1	25	theme	high	214:217	arg1	EPS					238:240	EPS	238:240	EPS	238:240	Streptococcus thermophilus CS6 could produce the high exopolysaccharide (EPS) level in optimized skimmed milk medium.
34655584	4	26	theme	1 → 3	935:939	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	27	theme	monosaccharide	519:532	arg1	composition					534:544	Further monosaccharide composition	511:544	Further monosaccharide composition	511:544	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	28	theme	1 → 3	830:834	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	6	29	theme	S.	1211:1212	arg1	CS6					1227:1229	a potential functional starter S. thermophilus CS6	1180:1229	a potential functional starter S. thermophilus CS6	1180:1229	Overall, EPS-M2 from a potential functional starter S. thermophilus CS6 provided opportunities for natural thickener, stabilizer, and antioxidant agent exploration in the food industry.
34655584	4	30	theme	α-L-Araf-	996:1004	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	1	31	theme	exopolysaccharide	219:235	arg1	level					243:247	the high exopolysaccharide (EPS) level	210:247	the high exopolysaccharide (EPS) level in optimized skimmed milk medium	210:280	Streptococcus thermophilus CS6 could produce the high exopolysaccharide (EPS) level in optimized skimmed milk medium.
34655584	4	32	theme	1 → 3	759:763	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	5	33	theme	key	1115:1117	arg1	enzymes					1119:1125	key enzymes	1115:1125	key enzymes of nucleotide sugar metabolism	1115:1156	High EPS production relied on the expression of eps gene cluster and key enzymes of nucleotide sugar metabolism.
34655584	3	34	theme	good	454:457	arg1	rheology					459:466	good rheology	454:466	good rheology	454:466	In this study, two purified fractions (EPS-M1 and EPS-M2) exhibited good rheology, thermostability and antioxidant activity.
34655584	6	35	theme	natural	1258:1264	arg1	thickener					1266:1274	natural thickener	1258:1274	natural thickener	1258:1274	Overall, EPS-M2 from a potential functional starter S. thermophilus CS6 provided opportunities for natural thickener, stabilizer, and antioxidant agent exploration in the food industry.
34655584	6	36	theme	functional	1192:1201	arg1	CS6					1227:1229	a potential functional starter S. thermophilus CS6	1180:1229	a potential functional starter S. thermophilus CS6	1180:1229	Overall, EPS-M2 from a potential functional starter S. thermophilus CS6 provided opportunities for natural thickener, stabilizer, and antioxidant agent exploration in the food industry.
34655584	2	37	theme	polymers	342:349	arg1	structure					323:331	structure	323:331	structure	323:331	However, physicochemical properties and structure of these polymers have not been fully characterized.
34655584	2	37	theme	polymers	342:349	arg1	properties					308:317	physicochemical properties	292:317	physicochemical properties	292:317	However, physicochemical properties and structure of these polymers have not been fully characterized.
34655584	4	38	theme	-α-L-Araf-	977:986	arg1	-α-L-Araf-					1013:1022	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	3	39	theme	antioxidant	489:499	arg1	activity					501:508	antioxidant activity	489:508	antioxidant activity	489:508	In this study, two purified fractions (EPS-M1 and EPS-M2) exhibited good rheology, thermostability and antioxidant activity.
34655584	3	40	theme	purified	405:412	arg1	fractions					414:422	two purified fractions	401:422	two purified fractions (EPS-M1 and EPS-M2)	401:442	In this study, two purified fractions (EPS-M1 and EPS-M2) exhibited good rheology, thermostability and antioxidant activity.
34655584	3	40	theme	purified	405:412	arg1	EPS-M2					436:441	EPS-M2	436:441	EPS-M2	436:441	In this study, two purified fractions (EPS-M1 and EPS-M2) exhibited good rheology, thermostability and antioxidant activity.
34655584	3	40	theme	purified	405:412	arg1	EPS-M1					425:430	EPS-M1	425:430	EPS-M1	425:430	In this study, two purified fractions (EPS-M1 and EPS-M2) exhibited good rheology, thermostability and antioxidant activity.
34655584	4	41	theme	galactose	614:622	arg1	5:2.5:1					648:654	5:2.5:1	648:654	5:2.5:1	648:654	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	41	theme	galactose	614:622	arg1	-β-D-Galp-					1031:1040	galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→	614:1043	-β-D-Galp-	1031:1040	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	6	42	theme	food	1330:1333	arg1	industry					1335:1342	the food industry	1326:1342	the food industry	1326:1342	Overall, EPS-M2 from a potential functional starter S. thermophilus CS6 provided opportunities for natural thickener, stabilizer, and antioxidant agent exploration in the food industry.
34655584	4	43	theme	1 → 5	918:922	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	44	theme	-β-D-Galp-	872:881	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	5	45	theme	High	1046:1049	arg1	production					1055:1064	High EPS production	1046:1064	High EPS production	1046:1064	High EPS production relied on the expression of eps gene cluster and key enzymes of nucleotide sugar metabolism.
34655584	5	46	theme	nucleotide	1130:1139	arg1	metabolism					1147:1156	nucleotide sugar metabolism	1130:1156	nucleotide sugar metabolism	1130:1156	High EPS production relied on the expression of eps gene cluster and key enzymes of nucleotide sugar metabolism.
34655584	0	47	from	CS6	160:162	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure, physicochemical characterization, and antioxidant activity of the highly arabinose-branched exopolysaccharide EPS-M2 from Streptococcus thermophilus CS6.
34655584	0	47	from	CS6	160:162	arg1	characterization					27:42	physicochemical characterization	11:42	physicochemical characterization	11:42	Structure, physicochemical characterization, and antioxidant activity of the highly arabinose-branched exopolysaccharide EPS-M2 from Streptococcus thermophilus CS6.
34655584	0	47	from	CS6	160:162	arg1	activity					61:68	antioxidant activity	49:68	antioxidant activity	49:68	Structure, physicochemical characterization, and antioxidant activity of the highly arabinose-branched exopolysaccharide EPS-M2 from Streptococcus thermophilus CS6.
34655584	1	48	from	level	243:247	arg1	medium					275:280	optimized skimmed milk medium	252:280	optimized skimmed milk medium	252:280	Streptococcus thermophilus CS6 could produce the high exopolysaccharide (EPS) level in optimized skimmed milk medium.
34655584	4	49	theme	-β-D-Galp-	766:775	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	50	theme	-α-L-Araf-	924:933	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	51	theme	1 → 6	883:887	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	52	with	5:2.5:1	648:654	arg1	weight					683:688	an average molecular weight	662:688	an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	662:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	0	53	theme	antioxidant	49:59	arg1	activity					61:68	antioxidant activity	49:68	antioxidant activity	49:68	Structure, physicochemical characterization, and antioxidant activity of the highly arabinose-branched exopolysaccharide EPS-M2 from Streptococcus thermophilus CS6.
34655584	4	54	theme	1 → 3	848:852	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	6	55	from	CS6	1227:1229	arg1	EPS-M2					1168:1173	EPS-M2	1168:1173	EPS-M2 from a potential functional starter S. thermophilus CS6	1168:1229	Overall, EPS-M2 from a potential functional starter S. thermophilus CS6 provided opportunities for natural thickener, stabilizer, and antioxidant agent exploration in the food industry.
34655584	6	56	theme	agent	1305:1309	arg1	exploration					1311:1321	antioxidant agent exploration	1293:1321	antioxidant agent exploration	1293:1321	Overall, EPS-M2 from a potential functional starter S. thermophilus CS6 provided opportunities for natural thickener, stabilizer, and antioxidant agent exploration in the food industry.
34655584	4	57	theme	→6	744:745	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	5	58	theme	eps	1094:1096	arg1	cluster					1103:1109	eps gene cluster	1094:1109	eps gene cluster	1094:1109	High EPS production relied on the expression of eps gene cluster and key enzymes of nucleotide sugar metabolism.
34655584	5	59	theme	EPS	1051:1053	arg1	production					1055:1064	High EPS production	1046:1064	High EPS production	1046:1064	High EPS production relied on the expression of eps gene cluster and key enzymes of nucleotide sugar metabolism.
34655584	4	60	theme	-β-D-Galp-	855:864	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	2	61	theme	physicochemical	292:306	arg1	properties					308:317	physicochemical properties	292:317	physicochemical properties	292:317	However, physicochemical properties and structure of these polymers have not been fully characterized.
34655584	4	62	theme	-[β-D-Galp-	959:969	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	1	63	theme	optimized	252:260	arg1	medium					275:280	optimized skimmed milk medium	252:280	optimized skimmed milk medium	252:280	Streptococcus thermophilus CS6 could produce the high exopolysaccharide (EPS) level in optimized skimmed milk medium.
34655584	6	64	from	opportunities	1240:1252	arg1	industry					1335:1342	the food industry	1326:1342	the food industry	1326:1342	Overall, EPS-M2 from a potential functional starter S. thermophilus CS6 provided opportunities for natural thickener, stabilizer, and antioxidant agent exploration in the food industry.
34655584	4	65	theme	-[α-L-Araf-	747:757	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	5	66	theme	cluster	1103:1109	arg1	expression					1080:1089	the expression	1076:1089	the expression of eps gene cluster	1076:1109	High EPS production relied on the expression of eps gene cluster and key enzymes of nucleotide sugar metabolism.
34655584	5	66	theme	cluster	1103:1109	arg1	enzymes					1119:1125	key enzymes	1115:1125	key enzymes of nucleotide sugar metabolism	1115:1156	High EPS production relied on the expression of eps gene cluster and key enzymes of nucleotide sugar metabolism.
34655584	1	67	theme	skimmed	262:268	arg1	medium					275:280	optimized skimmed milk medium	252:280	optimized skimmed milk medium	252:280	Streptococcus thermophilus CS6 could produce the high exopolysaccharide (EPS) level in optimized skimmed milk medium.
34655584	4	68	theme	1 → 5	901:905	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	69	theme	molecular	673:681	arg1	weight					683:688	an average molecular weight	662:688	an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	662:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	70	theme	-[α-L-Araf-	800:810	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	71	theme	average	665:671	arg1	weight					683:688	an average molecular weight	662:688	an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	662:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	72	theme	repeating	725:733	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	73	theme	1 → 5	971:975	arg1	-α-L-Araf-					977:986	repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)	725:993	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	1	74	theme	milk	270:273	arg1	medium					275:280	optimized skimmed milk medium	252:280	optimized skimmed milk medium	252:280	Streptococcus thermophilus CS6 could produce the high exopolysaccharide (EPS) level in optimized skimmed milk medium.
34655584	6	75	theme	starter	1203:1209	arg1	CS6					1227:1229	a potential functional starter S. thermophilus CS6	1180:1229	a potential functional starter S. thermophilus CS6	1180:1229	Overall, EPS-M2 from a potential functional starter S. thermophilus CS6 provided opportunities for natural thickener, stabilizer, and antioxidant agent exploration in the food industry.
34655584	4	76	theme	molecular	547:555	arg1	weight					557:562	molecular weight	547:562	molecular weight	547:562	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	0	77	theme	physicochemical	11:25	arg1	characterization					27:42	physicochemical characterization	11:42	physicochemical characterization	11:42	Structure, physicochemical characterization, and antioxidant activity of the highly arabinose-branched exopolysaccharide EPS-M2 from Streptococcus thermophilus CS6.
34655584	4	78	theme	-α-L-Araf-	1013:1022	arg1	weight					683:688	an average molecular weight	662:688	an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	662:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	4	79	theme	suggested	715:723	arg1	-α-L-Araf-					1013:1022	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)	711:1029	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
34655584	6	80	theme	antioxidant	1293:1303	arg1	exploration					1311:1321	antioxidant agent exploration	1293:1321	antioxidant agent exploration	1293:1321	Overall, EPS-M2 from a potential functional starter S. thermophilus CS6 provided opportunities for natural thickener, stabilizer, and antioxidant agent exploration in the food industry.
34655584	4	81	theme	NMR	568:570	arg1	analysis					572:579	NMR analysis	568:579	NMR analysis	568:579	Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→.
33180933	7	0	theme	wall	1277:1280	arg1	porosity					1282:1289	cell wall porosity	1272:1289	cell wall porosity	1272:1289	The results were complemented by expression analysis of cell wall synthesis genes and microscopic analysis of cell wall porosity.
33180933	5	1	theme	wild-type	811:819	arg1	plants					821:826	wild-type plants	811:826	wild-type plants	811:826	Mutants with reduced pectin or hemicellulose content showed no root cell elongation in response to simulated drought stress, in contrast to wild-type plants or mutants with reduced cellulose content.
33180933	6	2	theme	elongation	962:971	arg1	degrees					914:920	the degrees	910:920	the degrees of pectin methylesterification and cell elongation	910:971	While no association was found between the degrees of pectin methylesterification and cell elongation, cell wall composition analysis suggested an important role of the pectin rhamnogalacturonan II (RGII), which was corroborated in experiments with the RGII-modifying chemical 2β-deoxy-Kdo.
33180933	6	3	theme	chemical	1139:1146	arg1	2β-deoxy-Kdo					1148:1159	the RGII-modifying chemical 2β-deoxy-Kdo	1120:1159	the RGII-modifying chemical 2β-deoxy-Kdo	1120:1159	While no association was found between the degrees of pectin methylesterification and cell elongation, cell wall composition analysis suggested an important role of the pectin rhamnogalacturonan II (RGII), which was corroborated in experiments with the RGII-modifying chemical 2β-deoxy-Kdo.
33180933	4	4	theme	biochemical	577:587	arg1	methods					613:619	biochemical and immunohistochemical methods	577:619	biochemical and immunohistochemical methods	577:619	Here, cell wall-related mutants of the model plant Arabidopsis were characterized by biochemical and immunohistochemical methods and Fourier-transform infrared microspectroscopy.
33180933	5	5	theme	simulated	770:778	arg1	stress					788:793	simulated drought stress	770:793	simulated drought stress	770:793	Mutants with reduced pectin or hemicellulose content showed no root cell elongation in response to simulated drought stress, in contrast to wild-type plants or mutants with reduced cellulose content.
33180933	4	6	theme	wall-related	503:514	arg1	mutants					516:522	cell wall-related mutants	498:522	cell wall-related mutants of the model plant Arabidopsis	498:553	Here, cell wall-related mutants of the model plant Arabidopsis were characterized by biochemical and immunohistochemical methods and Fourier-transform infrared microspectroscopy.
33180933	5	7	theme	hemicellulose	702:714	arg1	content					716:722	reduced pectin or hemicellulose content	684:722	content	716:722	Mutants with reduced pectin or hemicellulose content showed no root cell elongation in response to simulated drought stress, in contrast to wild-type plants or mutants with reduced cellulose content.
33180933	5	8	with	mutants	831:837	arg1	content					862:868	reduced cellulose content	844:868	reduced cellulose content	844:868	Mutants with reduced pectin or hemicellulose content showed no root cell elongation in response to simulated drought stress, in contrast to wild-type plants or mutants with reduced cellulose content.
33180933	6	9	theme	pectin	1040:1045	arg1	rhamnogalacturonan					1047:1064	the pectin rhamnogalacturonan II	1036:1067	the pectin rhamnogalacturonan II (RGII)	1036:1074	While no association was found between the degrees of pectin methylesterification and cell elongation, cell wall composition analysis suggested an important role of the pectin rhamnogalacturonan II (RGII), which was corroborated in experiments with the RGII-modifying chemical 2β-deoxy-Kdo.
33180933	6	9	theme	pectin	1040:1045	arg1	RGII					1070:1073	RGII	1070:1073	RGII	1070:1073	While no association was found between the degrees of pectin methylesterification and cell elongation, cell wall composition analysis suggested an important role of the pectin rhamnogalacturonan II (RGII), which was corroborated in experiments with the RGII-modifying chemical 2β-deoxy-Kdo.
33180933	5	10	theme	drought	780:786	arg1	stress					788:793	simulated drought stress	770:793	simulated drought stress	770:793	Mutants with reduced pectin or hemicellulose content showed no root cell elongation in response to simulated drought stress, in contrast to wild-type plants or mutants with reduced cellulose content.
33180933	6	11	theme	composition	984:994	arg1	analysis					996:1003	cell wall composition analysis	974:1003	cell wall composition analysis	974:1003	While no association was found between the degrees of pectin methylesterification and cell elongation, cell wall composition analysis suggested an important role of the pectin rhamnogalacturonan II (RGII), which was corroborated in experiments with the RGII-modifying chemical 2β-deoxy-Kdo.
33180933	3	12	theme	structure	481:489	arg1	abundance					423:431	the abundance	419:431	the abundance of certain pectins, their modifications, and/or structure	419:489	Pectins have been implicated in this process, but it remains unclear if this depends on the abundance of certain pectins, their modifications, and/or structure.
33180933	7	13	theme	microscopic	1248:1258	arg1	analysis					1260:1267	microscopic analysis	1248:1267	microscopic analysis of cell wall porosity	1248:1289	The results were complemented by expression analysis of cell wall synthesis genes and microscopic analysis of cell wall porosity.
33180933	6	14	theme	methylesterification	932:951	arg1	degrees					914:920	the degrees	910:920	the degrees of pectin methylesterification and cell elongation	910:971	While no association was found between the degrees of pectin methylesterification and cell elongation, cell wall composition analysis suggested an important role of the pectin rhamnogalacturonan II (RGII), which was corroborated in experiments with the RGII-modifying chemical 2β-deoxy-Kdo.
33180933	5	15	theme	reduced	844:850	arg1	content					862:868	reduced cellulose content	844:868	reduced cellulose content	844:868	Mutants with reduced pectin or hemicellulose content showed no root cell elongation in response to simulated drought stress, in contrast to wild-type plants or mutants with reduced cellulose content.
33180933	5	16	with	plants	821:826	arg1	content					862:868	reduced cellulose content	844:868	reduced cellulose content	844:868	Mutants with reduced pectin or hemicellulose content showed no root cell elongation in response to simulated drought stress, in contrast to wild-type plants or mutants with reduced cellulose content.
33180933	3	17	theme	certain	436:442	arg1	pectins					444:450	certain pectins	436:450	certain pectins	436:450	Pectins have been implicated in this process, but it remains unclear if this depends on the abundance of certain pectins, their modifications, and/or structure.
33180933	4	18	theme	immunohistochemical	593:611	arg1	methods					613:619	biochemical and immunohistochemical methods	577:619	biochemical and immunohistochemical methods	577:619	Here, cell wall-related mutants of the model plant Arabidopsis were characterized by biochemical and immunohistochemical methods and Fourier-transform infrared microspectroscopy.
33180933	6	19	theme	pectin	925:930	arg1	methylesterification					932:951	pectin methylesterification	925:951	pectin methylesterification	925:951	While no association was found between the degrees of pectin methylesterification and cell elongation, cell wall composition analysis suggested an important role of the pectin rhamnogalacturonan II (RGII), which was corroborated in experiments with the RGII-modifying chemical 2β-deoxy-Kdo.
33180933	7	20	theme	expression	1195:1204	arg1	analysis					1206:1213	expression analysis	1195:1213	expression analysis of cell wall synthesis genes	1195:1242	The results were complemented by expression analysis of cell wall synthesis genes and microscopic analysis of cell wall porosity.
33180933	2	21	theme	cellulose	279:287	arg1	microfibrils					289:300	cellulose microfibrils	279:300	cellulose microfibrils	279:300	Cell extension involves loosening the bonds between cellulose microfibrils, hemicelluloses and pectins.
33180933	3	22	theme	pectins	444:450	arg1	abundance					423:431	the abundance	419:431	the abundance of certain pectins, their modifications, and/or structure	419:489	Pectins have been implicated in this process, but it remains unclear if this depends on the abundance of certain pectins, their modifications, and/or structure.
33180933	0	23	theme	pectin	8:13	arg1	content					15:21	Reduced pectin content	0:21	Reduced pectin content of cell walls	0:35	Reduced pectin content of cell walls prevents stress-induced root cell elongation in Arabidopsis.
33180933	7	24	theme	porosity	1282:1289	arg1	analysis					1206:1213	expression analysis	1195:1213	expression analysis of cell wall synthesis genes	1195:1242	The results were complemented by expression analysis of cell wall synthesis genes and microscopic analysis of cell wall porosity.
33180933	7	24	theme	porosity	1282:1289	arg1	analysis					1260:1267	microscopic analysis	1248:1267	microscopic analysis of cell wall porosity	1248:1289	The results were complemented by expression analysis of cell wall synthesis genes and microscopic analysis of cell wall porosity.
33180933	5	25	dep	plants	821:826	arg1	contrast					799:806	contrast	799:806	contrast	799:806	Mutants with reduced pectin or hemicellulose content showed no root cell elongation in response to simulated drought stress, in contrast to wild-type plants or mutants with reduced cellulose content.
33180933	7	26	theme	genes	1238:1242	arg1	analysis					1206:1213	expression analysis	1195:1213	expression analysis of cell wall synthesis genes	1195:1242	The results were complemented by expression analysis of cell wall synthesis genes and microscopic analysis of cell wall porosity.
33180933	7	26	theme	genes	1238:1242	arg1	analysis					1260:1267	microscopic analysis	1248:1267	microscopic analysis of cell wall porosity	1248:1289	The results were complemented by expression analysis of cell wall synthesis genes and microscopic analysis of cell wall porosity.
33180933	0	27	theme	Reduced	0:6	arg1	content					15:21	Reduced pectin content	0:21	Reduced pectin content of cell walls	0:35	Reduced pectin content of cell walls prevents stress-induced root cell elongation in Arabidopsis.
33180933	7	28	theme	cell	1218:1221	arg1	genes					1238:1242	cell wall synthesis genes	1218:1242	cell wall synthesis genes	1218:1242	The results were complemented by expression analysis of cell wall synthesis genes and microscopic analysis of cell wall porosity.
33180933	5	29	theme	reduced	684:690	arg1	pectin					692:697	reduced pectin or hemicellulose content	684:722	pectin	692:697	Mutants with reduced pectin or hemicellulose content showed no root cell elongation in response to simulated drought stress, in contrast to wild-type plants or mutants with reduced cellulose content.
33180933	5	30	theme	cellulose	852:860	arg1	content					862:868	reduced cellulose content	844:868	reduced cellulose content	844:868	Mutants with reduced pectin or hemicellulose content showed no root cell elongation in response to simulated drought stress, in contrast to wild-type plants or mutants with reduced cellulose content.
33180933	8	31	theme	certain	1315:1321	arg1	amount					1323:1328	a certain amount	1313:1328	a certain amount of pectin	1313:1338	It is concluded that a certain amount of pectin is necessary for stress-induced root cell elongation, and hypotheses regarding the mechanistic basis of this result are formulated.
33180933	8	31	theme	certain	1315:1321	arg1	necessary					1343:1351	necessary	1343:1351	necessary	1343:1351	It is concluded that a certain amount of pectin is necessary for stress-induced root cell elongation, and hypotheses regarding the mechanistic basis of this result are formulated.
33180933	8	31	theme	certain	1315:1321	arg1	pectin					1333:1338	pectin	1333:1338	pectin	1333:1338	It is concluded that a certain amount of pectin is necessary for stress-induced root cell elongation, and hypotheses regarding the mechanistic basis of this result are formulated.
33180933	7	32	theme	wall	1223:1226	arg1	genes					1238:1242	cell wall synthesis genes	1218:1242	cell wall synthesis genes	1218:1242	The results were complemented by expression analysis of cell wall synthesis genes and microscopic analysis of cell wall porosity.
33180933	4	33	theme	Arabidopsis	543:553	arg1	mutants					516:522	cell wall-related mutants	498:522	cell wall-related mutants of the model plant Arabidopsis	498:553	Here, cell wall-related mutants of the model plant Arabidopsis were characterized by biochemical and immunohistochemical methods and Fourier-transform infrared microspectroscopy.
33180933	3	34	theme	modifications	459:471	arg1	abundance					423:431	the abundance	419:431	the abundance of certain pectins, their modifications, and/or structure	419:489	Pectins have been implicated in this process, but it remains unclear if this depends on the abundance of certain pectins, their modifications, and/or structure.
33180933	7	35	theme	synthesis	1228:1236	arg1	genes					1238:1242	cell wall synthesis genes	1218:1242	cell wall synthesis genes	1218:1242	The results were complemented by expression analysis of cell wall synthesis genes and microscopic analysis of cell wall porosity.
33180933	6	36	theme	important	1018:1026	arg1	role					1028:1031	an important role	1015:1031	an important role	1015:1031	While no association was found between the degrees of pectin methylesterification and cell elongation, cell wall composition analysis suggested an important role of the pectin rhamnogalacturonan II (RGII), which was corroborated in experiments with the RGII-modifying chemical 2β-deoxy-Kdo.
33180933	1	37	theme	primary	102:108	arg1	walls					115:119	The primary cell walls	98:119	The primary cell walls of plants	98:129	The primary cell walls of plants provide mechanical strength while maintaining the flexibility needed for cell extension growth.
33180933	1	38	theme	cell	204:207	arg1	growth					219:224	cell extension growth	204:224	cell extension growth	204:224	The primary cell walls of plants provide mechanical strength while maintaining the flexibility needed for cell extension growth.
33180933	0	39	theme	walls	31:35	arg1	content					15:21	Reduced pectin content	0:21	Reduced pectin content of cell walls	0:35	Reduced pectin content of cell walls prevents stress-induced root cell elongation in Arabidopsis.
33180933	6	40	with	experiments	1103:1113	arg1	2β-deoxy-Kdo					1148:1159	the RGII-modifying chemical 2β-deoxy-Kdo	1120:1159	the RGII-modifying chemical 2β-deoxy-Kdo	1120:1159	While no association was found between the degrees of pectin methylesterification and cell elongation, cell wall composition analysis suggested an important role of the pectin rhamnogalacturonan II (RGII), which was corroborated in experiments with the RGII-modifying chemical 2β-deoxy-Kdo.
33180933	1	41	theme	extension	209:217	arg1	growth					219:224	cell extension growth	204:224	cell extension growth	204:224	The primary cell walls of plants provide mechanical strength while maintaining the flexibility needed for cell extension growth.
33180933	0	42	theme	cell	26:29	arg1	walls					31:35	cell walls	26:35	cell walls	26:35	Reduced pectin content of cell walls prevents stress-induced root cell elongation in Arabidopsis.
33180933	6	43	theme	rhamnogalacturonan	1047:1064	arg1	role					1028:1031	an important role	1015:1031	an important role	1015:1031	While no association was found between the degrees of pectin methylesterification and cell elongation, cell wall composition analysis suggested an important role of the pectin rhamnogalacturonan II (RGII), which was corroborated in experiments with the RGII-modifying chemical 2β-deoxy-Kdo.
33180933	8	44	theme	root	1372:1375	arg1	elongation					1382:1391	stress-induced root cell elongation	1357:1391	stress-induced root cell elongation	1357:1391	It is concluded that a certain amount of pectin is necessary for stress-induced root cell elongation, and hypotheses regarding the mechanistic basis of this result are formulated.
33180933	6	45	theme	RGII-modifying	1124:1137	arg1	2β-deoxy-Kdo					1148:1159	the RGII-modifying chemical 2β-deoxy-Kdo	1120:1159	the RGII-modifying chemical 2β-deoxy-Kdo	1120:1159	While no association was found between the degrees of pectin methylesterification and cell elongation, cell wall composition analysis suggested an important role of the pectin rhamnogalacturonan II (RGII), which was corroborated in experiments with the RGII-modifying chemical 2β-deoxy-Kdo.
33180933	6	46	theme	wall	979:982	arg1	analysis					996:1003	cell wall composition analysis	974:1003	cell wall composition analysis	974:1003	While no association was found between the degrees of pectin methylesterification and cell elongation, cell wall composition analysis suggested an important role of the pectin rhamnogalacturonan II (RGII), which was corroborated in experiments with the RGII-modifying chemical 2β-deoxy-Kdo.
33180933	0	47	theme	stress-induced	46:59	arg1	elongation					71:80	stress-induced root cell elongation	46:80	stress-induced root cell elongation	46:80	Reduced pectin content of cell walls prevents stress-induced root cell elongation in Arabidopsis.
33180933	5	48	dep	stress	788:793	arg1	response					758:765	response	758:765	response	758:765	Mutants with reduced pectin or hemicellulose content showed no root cell elongation in response to simulated drought stress, in contrast to wild-type plants or mutants with reduced cellulose content.
33180933	2	49	theme	Cell	227:230	arg1	extension					232:240	Cell extension	227:240	Cell extension	227:240	Cell extension involves loosening the bonds between cellulose microfibrils, hemicelluloses and pectins.
33180933	8	50	theme	mechanistic	1423:1433	arg1	basis					1435:1439	the mechanistic basis	1419:1439	the mechanistic basis of this result	1419:1454	It is concluded that a certain amount of pectin is necessary for stress-induced root cell elongation, and hypotheses regarding the mechanistic basis of this result are formulated.
33180933	1	51	theme	cell	110:113	arg1	walls					115:119	The primary cell walls	98:119	The primary cell walls of plants	98:129	The primary cell walls of plants provide mechanical strength while maintaining the flexibility needed for cell extension growth.
33180933	6	52	theme	cell	957:960	arg1	elongation					962:971	cell elongation	957:971	cell elongation	957:971	While no association was found between the degrees of pectin methylesterification and cell elongation, cell wall composition analysis suggested an important role of the pectin rhamnogalacturonan II (RGII), which was corroborated in experiments with the RGII-modifying chemical 2β-deoxy-Kdo.
33180933	4	53	theme	plant	537:541	arg1	Arabidopsis					543:553	the model plant Arabidopsis	527:553	the model plant Arabidopsis	527:553	Here, cell wall-related mutants of the model plant Arabidopsis were characterized by biochemical and immunohistochemical methods and Fourier-transform infrared microspectroscopy.
33180933	6	54	theme	cell	974:977	arg1	analysis					996:1003	cell wall composition analysis	974:1003	cell wall composition analysis	974:1003	While no association was found between the degrees of pectin methylesterification and cell elongation, cell wall composition analysis suggested an important role of the pectin rhamnogalacturonan II (RGII), which was corroborated in experiments with the RGII-modifying chemical 2β-deoxy-Kdo.
33180933	7	55	theme	cell	1272:1275	arg1	porosity					1282:1289	cell wall porosity	1272:1289	cell wall porosity	1272:1289	The results were complemented by expression analysis of cell wall synthesis genes and microscopic analysis of cell wall porosity.
33180933	4	56	theme	model	531:535	arg1	Arabidopsis					543:553	the model plant Arabidopsis	527:553	the model plant Arabidopsis	527:553	Here, cell wall-related mutants of the model plant Arabidopsis were characterized by biochemical and immunohistochemical methods and Fourier-transform infrared microspectroscopy.
33180933	5	57	theme	root	734:737	arg1	elongation					744:753	no root cell elongation	731:753	no root cell elongation	731:753	Mutants with reduced pectin or hemicellulose content showed no root cell elongation in response to simulated drought stress, in contrast to wild-type plants or mutants with reduced cellulose content.
33180933	8	58	theme	result	1449:1454	arg1	basis					1435:1439	the mechanistic basis	1419:1439	the mechanistic basis of this result	1419:1454	It is concluded that a certain amount of pectin is necessary for stress-induced root cell elongation, and hypotheses regarding the mechanistic basis of this result are formulated.
33180933	8	59	theme	stress-induced	1357:1370	arg1	elongation					1382:1391	stress-induced root cell elongation	1357:1391	stress-induced root cell elongation	1357:1391	It is concluded that a certain amount of pectin is necessary for stress-induced root cell elongation, and hypotheses regarding the mechanistic basis of this result are formulated.
33180933	4	60	theme	Fourier-transform	625:641	arg1	microspectroscopy					652:668	Fourier-transform infrared microspectroscopy	625:668	Fourier-transform infrared microspectroscopy	625:668	Here, cell wall-related mutants of the model plant Arabidopsis were characterized by biochemical and immunohistochemical methods and Fourier-transform infrared microspectroscopy.
33180933	5	61	theme	cell	739:742	arg1	elongation					744:753	no root cell elongation	731:753	no root cell elongation	731:753	Mutants with reduced pectin or hemicellulose content showed no root cell elongation in response to simulated drought stress, in contrast to wild-type plants or mutants with reduced cellulose content.
33180933	8	62	theme	pectin	1333:1338	arg1	amount					1323:1328	a certain amount	1313:1328	a certain amount of pectin	1313:1338	It is concluded that a certain amount of pectin is necessary for stress-induced root cell elongation, and hypotheses regarding the mechanistic basis of this result are formulated.
33180933	8	62	theme	pectin	1333:1338	arg1	necessary					1343:1351	necessary	1343:1351	necessary	1343:1351	It is concluded that a certain amount of pectin is necessary for stress-induced root cell elongation, and hypotheses regarding the mechanistic basis of this result are formulated.
33180933	8	62	theme	pectin	1333:1338	arg1	pectin					1333:1338	pectin	1333:1338	pectin	1333:1338	It is concluded that a certain amount of pectin is necessary for stress-induced root cell elongation, and hypotheses regarding the mechanistic basis of this result are formulated.
33180933	1	63	theme	plants	124:129	arg1	walls					115:119	The primary cell walls	98:119	The primary cell walls of plants	98:129	The primary cell walls of plants provide mechanical strength while maintaining the flexibility needed for cell extension growth.
33180933	0	64	theme	cell	66:69	arg1	elongation					71:80	stress-induced root cell elongation	46:80	stress-induced root cell elongation	46:80	Reduced pectin content of cell walls prevents stress-induced root cell elongation in Arabidopsis.
33180933	8	65	theme	cell	1377:1380	arg1	elongation					1382:1391	stress-induced root cell elongation	1357:1391	stress-induced root cell elongation	1357:1391	It is concluded that a certain amount of pectin is necessary for stress-induced root cell elongation, and hypotheses regarding the mechanistic basis of this result are formulated.
33180933	0	66	theme	root	61:64	arg1	elongation					71:80	stress-induced root cell elongation	46:80	stress-induced root cell elongation	46:80	Reduced pectin content of cell walls prevents stress-induced root cell elongation in Arabidopsis.
33180933	4	67	theme	cell	498:501	arg1	mutants					516:522	cell wall-related mutants	498:522	cell wall-related mutants of the model plant Arabidopsis	498:553	Here, cell wall-related mutants of the model plant Arabidopsis were characterized by biochemical and immunohistochemical methods and Fourier-transform infrared microspectroscopy.
33180933	5	68	with	Mutants	671:677	arg1	pectin					692:697	reduced pectin or hemicellulose content	684:722	pectin	692:697	Mutants with reduced pectin or hemicellulose content showed no root cell elongation in response to simulated drought stress, in contrast to wild-type plants or mutants with reduced cellulose content.
33180933	5	68	with	Mutants	671:677	arg1	content					716:722	reduced pectin or hemicellulose content	684:722	content	716:722	Mutants with reduced pectin or hemicellulose content showed no root cell elongation in response to simulated drought stress, in contrast to wild-type plants or mutants with reduced cellulose content.
33180933	1	69	theme	mechanical	139:148	arg1	strength					150:157	mechanical strength	139:157	mechanical strength	139:157	The primary cell walls of plants provide mechanical strength while maintaining the flexibility needed for cell extension growth.
33180933	4	70	theme	infrared	643:650	arg1	microspectroscopy					652:668	Fourier-transform infrared microspectroscopy	625:668	Fourier-transform infrared microspectroscopy	625:668	Here, cell wall-related mutants of the model plant Arabidopsis were characterized by biochemical and immunohistochemical methods and Fourier-transform infrared microspectroscopy.
34420751	7	0	theme	chromatographic	1241:1255	arg1	media					1257:1261	next-generation chromatographic media	1225:1261	next-generation chromatographic media of natural polysaccharide-based cryogel for protein purification	1225:1326	This work provides a new avenue to design and develop next-generation chromatographic media of natural polysaccharide-based cryogel for protein purification.
34420751	5	1	theme	white	997:1001	arg1	egg					993:995	chicken egg white	985:1001	chicken egg white	985:1001	Furthermore, the cryogel-packed column can purify lysozyme continuously from chicken egg white, highlighting its outstanding practical application performance.
34420751	6	2	theme	lysozyme	1113:1120	arg1	capacity					1133:1140	lysozyme extraction capacity	1113:1140	lysozyme extraction capacity	1113:1140	Reuse experiments indicated that over 90% of lysozyme extraction capacity was retained after 6 cycles.
34420751	2	3	theme	SA	404:405	arg1	cryogels					408:415	microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels	354:415	microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels	354:415	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	0	4	theme	protein	132:138	arg1	purification					140:151	efficient protein purification	122:151	efficient protein purification	122:151	Shapeable and underwater super-elastic cellulose nanofiber/alginate cryogels by freezing-induced oxa-Michael reaction for efficient protein purification.
34420751	2	5	theme	SAs	650:652	arg1	DVS					614:616	DVS	614:616	DVS	614:616	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	2	5	theme	SAs	650:652	arg1	groups					631:636	hydroxyl groups	622:636	hydroxyl groups	622:636	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	2	5	theme	SAs	650:652	arg1	SAs					650:652	SAs	650:652	SAs	650:652	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	2	5	theme	SAs	650:652	arg1	MFCs					641:644	MFCs	641:644	MFCs	641:644	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	0	6	theme	efficient	122:130	arg1	purification					140:151	efficient protein purification	122:151	efficient protein purification	122:151	Shapeable and underwater super-elastic cellulose nanofiber/alginate cryogels by freezing-induced oxa-Michael reaction for efficient protein purification.
34420751	7	7	theme	next-generation	1225:1239	arg1	media					1257:1261	next-generation chromatographic media	1225:1261	next-generation chromatographic media of natural polysaccharide-based cryogel for protein purification	1225:1326	This work provides a new avenue to design and develop next-generation chromatographic media of natural polysaccharide-based cryogel for protein purification.
34420751	4	8	theme	294.12 mg/g	835:845	arg1	capacity					823:830	a high lysozyme adsorption capacity	796:830	a high lysozyme adsorption capacity of 294.12 mg/g	796:845	Moreover, the MFC/SAs reveal a high lysozyme adsorption capacity of 294.12 mg/g, surpassing most of absorbent materials previously reported.
34420751	5	9	theme	practical	1033:1041	arg1	application					1043:1053	its outstanding practical application	1017:1053	its outstanding practical application performance	1017:1065	Furthermore, the cryogel-packed column can purify lysozyme continuously from chicken egg white, highlighting its outstanding practical application performance.
34420751	4	10	theme	adsorption	812:821	arg1	capacity					823:830	a high lysozyme adsorption capacity	796:830	a high lysozyme adsorption capacity of 294.12 mg/g	796:845	Moreover, the MFC/SAs reveal a high lysozyme adsorption capacity of 294.12 mg/g, surpassing most of absorbent materials previously reported.
34420751	5	11	theme	outstanding	1021:1031	arg1	application					1043:1053	its outstanding practical application	1017:1053	its outstanding practical application performance	1017:1065	Furthermore, the cryogel-packed column can purify lysozyme continuously from chicken egg white, highlighting its outstanding practical application performance.
34420751	7	12	theme	natural	1266:1272	arg1	cryogel					1295:1301	natural polysaccharide-based cryogel	1266:1301	natural polysaccharide-based cryogel for protein purification	1266:1326	This work provides a new avenue to design and develop next-generation chromatographic media of natural polysaccharide-based cryogel for protein purification.
34420751	7	13	theme	polysaccharide-based	1274:1293	arg1	cryogel					1295:1301	natural polysaccharide-based cryogel	1266:1301	natural polysaccharide-based cryogel for protein purification	1266:1326	This work provides a new avenue to design and develop next-generation chromatographic media of natural polysaccharide-based cryogel for protein purification.
34420751	5	14	theme	application	1043:1053	arg1	performance					1055:1065	its outstanding practical application performance	1017:1065	its outstanding practical application performance	1017:1065	Furthermore, the cryogel-packed column can purify lysozyme continuously from chicken egg white, highlighting its outstanding practical application performance.
34420751	2	15	theme	oxa-Michael	443:453	arg1	reaction					455:462	freezing-induced oxa-Michael reaction	426:462	freezing-induced oxa-Michael reaction	426:462	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	0	16	theme	oxa-Michael	97:107	arg1	reaction					109:116	freezing-induced oxa-Michael reaction	80:116	freezing-induced oxa-Michael reaction for efficient protein purification	80:151	Shapeable and underwater super-elastic cellulose nanofiber/alginate cryogels by freezing-induced oxa-Michael reaction for efficient protein purification.
34420751	2	17	theme	MFC	382:384	arg1	cryogels					408:415	microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels	354:415	microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels	354:415	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	2	18	theme	freezing-induced	426:441	arg1	reaction					455:462	freezing-induced oxa-Michael reaction	426:462	freezing-induced oxa-Michael reaction	426:462	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	0	19	theme	Shapeable	0:8	arg1	nanofiber/alginate					49:66	Shapeable and underwater super-elastic cellulose nanofiber/alginate	0:66	Shapeable and underwater super-elastic cellulose nanofiber/alginate	0:66	Shapeable and underwater super-elastic cellulose nanofiber/alginate cryogels by freezing-induced oxa-Michael reaction for efficient protein purification.
34420751	3	20	theme	fatigue	747:753	arg1	resistance					755:764	excellent fatigue resistance	737:764	excellent fatigue resistance	737:764	The obtained composite cryogel exhibits outstanding underwater shape recovery and excellent fatigue resistance.
34420751	1	21	theme	great	233:237	arg1	significance					239:250	great significance	233:250	great significance	233:250	Construction of monolithic cryogels that can efficiently adsorb proteins is of great significance in biotechnological and pharmaceutical industries.
34420751	0	22	theme	super-elastic	25:37	arg1	nanofiber/alginate					49:66	Shapeable and underwater super-elastic cellulose nanofiber/alginate	0:66	Shapeable and underwater super-elastic cellulose nanofiber/alginate	0:66	Shapeable and underwater super-elastic cellulose nanofiber/alginate cryogels by freezing-induced oxa-Michael reaction for efficient protein purification.
34420751	2	23	theme	cellulose	371:379	arg1	cryogels					408:415	microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels	354:415	microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels	354:415	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	4	24	theme	lysozyme	803:810	arg1	capacity					823:830	a high lysozyme adsorption capacity	796:830	a high lysozyme adsorption capacity of 294.12 mg/g	796:845	Moreover, the MFC/SAs reveal a high lysozyme adsorption capacity of 294.12 mg/g, surpassing most of absorbent materials previously reported.
34420751	7	25	theme	new	1192:1194	arg1	avenue					1196:1201	a new avenue	1190:1201	a new avenue to design	1190:1211	This work provides a new avenue to design and develop next-generation chromatographic media of natural polysaccharide-based cryogel for protein purification.
34420751	3	26	theme	shape	718:722	arg1	recovery					724:731	outstanding underwater shape recovery	695:731	outstanding underwater shape recovery	695:731	The obtained composite cryogel exhibits outstanding underwater shape recovery and excellent fatigue resistance.
34420751	2	27	theme	microfibrillated	354:369	arg1	cryogels					408:415	microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels	354:415	microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels	354:415	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	5	28	theme	cryogel-packed	925:938	arg1	column					940:945	the cryogel-packed column	921:945	the cryogel-packed column	921:945	Furthermore, the cryogel-packed column can purify lysozyme continuously from chicken egg white, highlighting its outstanding practical application performance.
34420751	2	29	theme	DVS	535:537	arg1	reactiveness					502:513	the controllable reactiveness	485:513	the controllable reactiveness of divinyl sulfone (DVS)	485:538	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	2	30	dep	-12 °C.	467:473	arg1	activates					571:579	activates	571:579	activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs	571:652	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	1	31	theme	biotechnological	255:270	arg1	industries					291:300	biotechnological and pharmaceutical industries	255:300	biotechnological and pharmaceutical industries	255:300	Construction of monolithic cryogels that can efficiently adsorb proteins is of great significance in biotechnological and pharmaceutical industries.
34420751	0	32	theme	cellulose	39:47	arg1	nanofiber/alginate					49:66	Shapeable and underwater super-elastic cellulose nanofiber/alginate	0:66	Shapeable and underwater super-elastic cellulose nanofiber/alginate	0:66	Shapeable and underwater super-elastic cellulose nanofiber/alginate cryogels by freezing-induced oxa-Michael reaction for efficient protein purification.
34420751	6	33	theme	Reuse	1068:1072	arg1	experiments					1074:1084	Reuse experiments	1068:1084	Reuse experiments	1068:1084	Reuse experiments indicated that over 90% of lysozyme extraction capacity was retained after 6 cycles.
34420751	7	34	theme	cryogel	1295:1301	arg1	media					1257:1261	next-generation chromatographic media	1225:1261	next-generation chromatographic media of natural polysaccharide-based cryogel for protein purification	1225:1326	This work provides a new avenue to design and develop next-generation chromatographic media of natural polysaccharide-based cryogel for protein purification.
34420751	3	35	theme	outstanding	695:705	arg1	recovery					724:731	outstanding underwater shape recovery	695:731	outstanding underwater shape recovery	695:731	The obtained composite cryogel exhibits outstanding underwater shape recovery and excellent fatigue resistance.
34420751	2	36	theme	controllable	489:500	arg1	reactiveness					502:513	the controllable reactiveness	485:513	the controllable reactiveness of divinyl sulfone (DVS)	485:538	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	2	37	theme	sulfone	526:532	arg1	DVS					535:537	divinyl sulfone (DVS)	518:538	divinyl sulfone (DVS)	518:538	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	2	38	theme	oxa-Michael	585:595	arg1	reaction					597:604	the oxa-Michael reaction	581:604	the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs	581:652	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	2	39	theme	MFCs	641:644	arg1	DVS					614:616	DVS	614:616	DVS	614:616	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	2	39	theme	MFCs	641:644	arg1	groups					631:636	hydroxyl groups	622:636	hydroxyl groups	622:636	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	2	39	theme	MFCs	641:644	arg1	SAs					650:652	SAs	650:652	SAs	650:652	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	2	39	theme	MFCs	641:644	arg1	MFCs					641:644	MFCs	641:644	MFCs	641:644	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	2	40	theme	cryo-concentrated	541:557	arg1	increase					562:569	cryo-concentrated pH increase	541:569	cryo-concentrated pH increase	541:569	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	2	41	theme	divinyl	518:524	arg1	DVS					535:537	divinyl sulfone (DVS)	518:538	divinyl sulfone (DVS)	518:538	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	3	42	theme	underwater	707:716	arg1	recovery					724:731	outstanding underwater shape recovery	695:731	outstanding underwater shape recovery	695:731	The obtained composite cryogel exhibits outstanding underwater shape recovery and excellent fatigue resistance.
34420751	1	43	from	significance	239:250	arg1	industries					291:300	biotechnological and pharmaceutical industries	255:300	biotechnological and pharmaceutical industries	255:300	Construction of monolithic cryogels that can efficiently adsorb proteins is of great significance in biotechnological and pharmaceutical industries.
34420751	2	44	theme	alginate	394:401	arg1	cryogels					408:415	microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels	354:415	microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels	354:415	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	4	45	theme	high	798:801	arg1	capacity					823:830	a high lysozyme adsorption capacity	796:830	a high lysozyme adsorption capacity of 294.12 mg/g	796:845	Moreover, the MFC/SAs reveal a high lysozyme adsorption capacity of 294.12 mg/g, surpassing most of absorbent materials previously reported.
34420751	4	46	theme	absorbent	867:875	arg1	materials					877:885	absorbent materials	867:885	absorbent materials previously reported	867:905	Moreover, the MFC/SAs reveal a high lysozyme adsorption capacity of 294.12 mg/g, surpassing most of absorbent materials previously reported.
34420751	1	47	theme	monolithic	170:179	arg1	cryogels					181:188	monolithic cryogels	170:188	monolithic cryogels that can efficiently adsorb proteins	170:225	Construction of monolithic cryogels that can efficiently adsorb proteins is of great significance in biotechnological and pharmaceutical industries.
34420751	1	48	theme	pharmaceutical	276:289	arg1	industries					291:300	biotechnological and pharmaceutical industries	255:300	biotechnological and pharmaceutical industries	255:300	Construction of monolithic cryogels that can efficiently adsorb proteins is of great significance in biotechnological and pharmaceutical industries.
34420751	2	49	theme	hydroxyl	622:629	arg1	groups					631:636	hydroxyl groups	622:636	hydroxyl groups	622:636	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	2	49	theme	hydroxyl	622:629	arg1	SAs					650:652	SAs	650:652	SAs	650:652	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	2	49	theme	hydroxyl	622:629	arg1	MFCs					641:644	MFCs	641:644	MFCs	641:644	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	2	50	theme	novel	313:317	arg1	approach					319:326	a novel approach	311:326	a novel approach	311:326	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	1	51	theme	cryogels	181:188	arg1	Construction					154:165	Construction	154:165	Construction of monolithic cryogels that can efficiently adsorb proteins	154:225	Construction of monolithic cryogels that can efficiently adsorb proteins is of great significance in biotechnological and pharmaceutical industries.
34420751	2	52	theme	/sodium	386:392	arg1	cryogels					408:415	microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels	354:415	microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels	354:415	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	2	53	theme	pH	559:560	arg1	increase					562:569	cryo-concentrated pH increase	541:569	cryo-concentrated pH increase	541:569	Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs.
34420751	3	54	theme	excellent	737:745	arg1	resistance					755:764	excellent fatigue resistance	737:764	excellent fatigue resistance	737:764	The obtained composite cryogel exhibits outstanding underwater shape recovery and excellent fatigue resistance.
34420751	3	55	theme	obtained	659:666	arg1	cryogel					678:684	The obtained composite cryogel	655:684	The obtained composite cryogel	655:684	The obtained composite cryogel exhibits outstanding underwater shape recovery and excellent fatigue resistance.
34420751	5	56	theme	chicken	985:991	arg1	egg					993:995	chicken egg white	985:1001	chicken egg white	985:1001	Furthermore, the cryogel-packed column can purify lysozyme continuously from chicken egg white, highlighting its outstanding practical application performance.
34420751	6	57	theme	capacity	1133:1140	arg1	capacity					1133:1140	lysozyme extraction capacity	1113:1140	lysozyme extraction capacity	1113:1140	Reuse experiments indicated that over 90% of lysozyme extraction capacity was retained after 6 cycles.
34420751	6	57	theme	capacity	1133:1140	arg1	%					1108:1108	over 90%	1101:1108	over 90% of lysozyme extraction capacity	1101:1140	Reuse experiments indicated that over 90% of lysozyme extraction capacity was retained after 6 cycles.
34420751	0	58	theme	freezing-induced	80:95	arg1	reaction					109:116	freezing-induced oxa-Michael reaction	80:116	freezing-induced oxa-Michael reaction for efficient protein purification	80:151	Shapeable and underwater super-elastic cellulose nanofiber/alginate cryogels by freezing-induced oxa-Michael reaction for efficient protein purification.
34420751	3	59	theme	composite	668:676	arg1	cryogel					678:684	The obtained composite cryogel	655:684	The obtained composite cryogel	655:684	The obtained composite cryogel exhibits outstanding underwater shape recovery and excellent fatigue resistance.
34420751	7	60	theme	protein	1307:1313	arg1	purification					1315:1326	protein purification	1307:1326	protein purification	1307:1326	This work provides a new avenue to design and develop next-generation chromatographic media of natural polysaccharide-based cryogel for protein purification.
34420751	6	61	theme	extraction	1122:1131	arg1	capacity					1133:1140	lysozyme extraction capacity	1113:1140	lysozyme extraction capacity	1113:1140	Reuse experiments indicated that over 90% of lysozyme extraction capacity was retained after 6 cycles.
34236836	0	0	theme	Microbial	73:81	arg1	Composition					83:93	Intestinal Microbial Composition	62:93	Intestinal Microbial Composition	62:93	Effects of Differences in Resistant Starch Content of Rice on Intestinal Microbial Composition.
34236836	0	1	from	Differences	11:21	arg1	Content					43:49	Resistant Starch Content	26:49	Resistant Starch Content of Rice	26:57	Effects of Differences in Resistant Starch Content of Rice on Intestinal Microbial Composition.
34236836	5	2	with	network	818:824	arg1	modularity					840:849	enhanced modularity	831:849	enhanced modularity	831:849	Moreover, increasing RS levels resulted in a molecular ecological network with enhanced modularity and interspecific synergy, which is less sensitive to high fat intervention.
34236836	5	2	with	network	818:824	arg1	synergy					869:875	interspecific synergy	855:875	interspecific synergy	855:875	Moreover, increasing RS levels resulted in a molecular ecological network with enhanced modularity and interspecific synergy, which is less sensitive to high fat intervention.
34236836	0	3	theme	Intestinal	62:71	arg1	Composition					83:93	Intestinal Microbial Composition	62:93	Intestinal Microbial Composition	62:93	Effects of Differences in Resistant Starch Content of Rice on Intestinal Microbial Composition.
34236836	6	4	from	rice	968:971	arg1	%					954:954	0.44%	950:954	0.44% from cooked rice	950:971	Overall, RS as low as 0.44% from cooked rice can modulate gut microbiome in mice, which correlated to a protective effect against deleterious effects of an obesogenic diet.
34236836	2	5	theme	major	282:286	arg1	levels					231:236	Three levels	225:236	Three levels of RS from three varieties of rice	225:271	Three levels of RS from three varieties of rice were the major source of carbohydrates and fat levels were low (10%) and high (39%).
34236836	2	5	theme	major	282:286	arg1	source					288:293	the major source	278:293	the major source of carbohydrates	278:310	Three levels of RS from three varieties of rice were the major source of carbohydrates and fat levels were low (10%) and high (39%).
34236836	2	6	from	varieties	255:263	arg1	levels					231:236	Three levels	225:236	Three levels of RS from three varieties of rice	225:271	Three levels of RS from three varieties of rice were the major source of carbohydrates and fat levels were low (10%) and high (39%).
34236836	2	6	from	varieties	255:263	arg1	source					288:293	the major source	278:293	the major source of carbohydrates	278:310	Three levels of RS from three varieties of rice were the major source of carbohydrates and fat levels were low (10%) and high (39%).
34236836	2	6	from	varieties	255:263	arg1	RS					241:242	RS	241:242	RS from three varieties of rice	241:271	Three levels of RS from three varieties of rice were the major source of carbohydrates and fat levels were low (10%) and high (39%).
34236836	0	7	from	Effects	0:6	arg1	Content					43:49	Resistant Starch Content	26:49	Resistant Starch Content of Rice	26:57	Effects of Differences in Resistant Starch Content of Rice on Intestinal Microbial Composition.
34236836	0	7	from	Effects	0:6	arg1	Composition					83:93	Intestinal Microbial Composition	62:93	Intestinal Microbial Composition	62:93	Effects of Differences in Resistant Starch Content of Rice on Intestinal Microbial Composition.
34236836	1	8	from	effects	138:144	arg1	microbiome					197:206	the gut microbiome	189:206	the gut microbiome in C57BL/6 mice	189:222	The aim of this study was to evaluate the effects of resistant starch (RS) and fat levels on the gut microbiome in C57BL/6 mice.
34236836	1	9	theme	C57BL/6	211:217	arg1	mice					219:222	C57BL/6 mice	211:222	C57BL/6 mice	211:222	The aim of this study was to evaluate the effects of resistant starch (RS) and fat levels on the gut microbiome in C57BL/6 mice.
34236836	0	10	from	Content	43:49	arg1	Effects					0:6	Effects	0:6	Effects of Differences in Resistant Starch Content of Rice on Intestinal Microbial Composition	0:93	Effects of Differences in Resistant Starch Content of Rice on Intestinal Microbial Composition.
34236836	3	11	theme	Bacteroidetes	407:419	arg1	ratio					421:425	Bacteroidetes ratio	407:425	Bacteroidetes ratio	407:425	We confirmed that RS decreased the Firmicutes to Bacteroidetes ratio, increased SCFA production by higher Bacteroidaceae and S24-7 abundance, and enriched predicted gene families of glycosidases and functional pathways associated with carbohydrate and glycan metabolism.
34236836	2	12	theme	fat	316:318	arg1	levels					320:325	fat levels	316:325	fat levels	316:325	Three levels of RS from three varieties of rice were the major source of carbohydrates and fat levels were low (10%) and high (39%).
34236836	4	13	theme	tissue	683:688	arg1	expression					695:704	tissue gene expression	683:704	tissue gene expression	683:704	We also found correlations between microbial taxa and tissue gene expression related to carbohydrate and lipid metabolism.
34236836	6	14	theme	gut	986:988	arg1	microbiome					990:999	gut microbiome	986:999	gut microbiome	986:999	Overall, RS as low as 0.44% from cooked rice can modulate gut microbiome in mice, which correlated to a protective effect against deleterious effects of an obesogenic diet.
34236836	1	15	theme	resistant	149:157	arg1	starch					159:164	resistant starch	149:164	resistant starch (RS)	149:169	The aim of this study was to evaluate the effects of resistant starch (RS) and fat levels on the gut microbiome in C57BL/6 mice.
34236836	1	15	theme	resistant	149:157	arg1	RS					167:168	RS	167:168	RS	167:168	The aim of this study was to evaluate the effects of resistant starch (RS) and fat levels on the gut microbiome in C57BL/6 mice.
34236836	1	16	theme	starch	159:164	arg1	effects					138:144	the effects	134:144	the effects of resistant starch (RS) and fat levels on the gut microbiome in C57BL/6 mice	134:222	The aim of this study was to evaluate the effects of resistant starch (RS) and fat levels on the gut microbiome in C57BL/6 mice.
34236836	6	17	theme	low	943:945	arg1	RS					937:938	RS	937:938	RS as low as 0.44% from cooked rice	937:971	Overall, RS as low as 0.44% from cooked rice can modulate gut microbiome in mice, which correlated to a protective effect against deleterious effects of an obesogenic diet.
34236836	2	18	dep	high	346:349	arg1	%					354:354	39%	352:354	39%	352:354	Three levels of RS from three varieties of rice were the major source of carbohydrates and fat levels were low (10%) and high (39%).
34236836	3	19	theme	higher	457:462	arg1	Bacteroidaceae					464:477	higher Bacteroidaceae	457:477	higher Bacteroidaceae	457:477	We confirmed that RS decreased the Firmicutes to Bacteroidetes ratio, increased SCFA production by higher Bacteroidaceae and S24-7 abundance, and enriched predicted gene families of glycosidases and functional pathways associated with carbohydrate and glycan metabolism.
34236836	6	20	theme	cooked	961:966	arg1	rice					968:971	cooked rice	961:971	cooked rice	961:971	Overall, RS as low as 0.44% from cooked rice can modulate gut microbiome in mice, which correlated to a protective effect against deleterious effects of an obesogenic diet.
34236836	3	21	theme	SCFA	438:441	arg1	production					443:452	SCFA production	438:452	SCFA production by higher Bacteroidaceae	438:477	We confirmed that RS decreased the Firmicutes to Bacteroidetes ratio, increased SCFA production by higher Bacteroidaceae and S24-7 abundance, and enriched predicted gene families of glycosidases and functional pathways associated with carbohydrate and glycan metabolism.
34236836	4	22	theme	lipid	734:738	arg1	metabolism					740:749	lipid metabolism	734:749	lipid metabolism	734:749	We also found correlations between microbial taxa and tissue gene expression related to carbohydrate and lipid metabolism.
34236836	0	23	theme	Differences	11:21	arg1	Effects					0:6	Effects	0:6	Effects of Differences in Resistant Starch Content of Rice on Intestinal Microbial Composition	0:93	Effects of Differences in Resistant Starch Content of Rice on Intestinal Microbial Composition.
34236836	4	24	theme	gene	690:693	arg1	expression					695:704	tissue gene expression	683:704	tissue gene expression	683:704	We also found correlations between microbial taxa and tissue gene expression related to carbohydrate and lipid metabolism.
34236836	2	25	theme	RS	241:242	arg1	levels					231:236	Three levels	225:236	Three levels of RS from three varieties of rice	225:271	Three levels of RS from three varieties of rice were the major source of carbohydrates and fat levels were low (10%) and high (39%).
34236836	2	25	theme	RS	241:242	arg1	source					288:293	the major source	278:293	the major source of carbohydrates	278:310	Three levels of RS from three varieties of rice were the major source of carbohydrates and fat levels were low (10%) and high (39%).
34236836	5	26	theme	molecular	797:805	arg1	network					818:824	a molecular ecological network	795:824	a molecular ecological network	795:824	Moreover, increasing RS levels resulted in a molecular ecological network with enhanced modularity and interspecific synergy, which is less sensitive to high fat intervention.
34236836	5	26	theme	molecular	797:805	arg1	sensitive					892:900	sensitive	892:900	sensitive	892:900	Moreover, increasing RS levels resulted in a molecular ecological network with enhanced modularity and interspecific synergy, which is less sensitive to high fat intervention.
34236836	0	27	theme	Starch	36:41	arg1	Content					43:49	Resistant Starch Content	26:49	Resistant Starch Content of Rice	26:57	Effects of Differences in Resistant Starch Content of Rice on Intestinal Microbial Composition.
34236836	1	28	from	microbiome	197:206	arg1	mice					219:222	C57BL/6 mice	211:222	C57BL/6 mice	211:222	The aim of this study was to evaluate the effects of resistant starch (RS) and fat levels on the gut microbiome in C57BL/6 mice.
34236836	3	29	theme	glycosidases	540:551	arg1	families					528:535	predicted gene families	513:535	predicted gene families of glycosidases and functional pathways associated with carbohydrate and glycan metabolism	513:626	We confirmed that RS decreased the Firmicutes to Bacteroidetes ratio, increased SCFA production by higher Bacteroidaceae and S24-7 abundance, and enriched predicted gene families of glycosidases and functional pathways associated with carbohydrate and glycan metabolism.
34236836	3	30	theme	S24-7	483:487	arg1	abundance					489:497	S24-7 abundance	483:497	S24-7 abundance	483:497	We confirmed that RS decreased the Firmicutes to Bacteroidetes ratio, increased SCFA production by higher Bacteroidaceae and S24-7 abundance, and enriched predicted gene families of glycosidases and functional pathways associated with carbohydrate and glycan metabolism.
34236836	5	31	theme	increasing	762:771	arg1	levels					776:781	increasing RS levels	762:781	increasing RS levels	762:781	Moreover, increasing RS levels resulted in a molecular ecological network with enhanced modularity and interspecific synergy, which is less sensitive to high fat intervention.
34236836	0	32	theme	Resistant	26:34	arg1	Content					43:49	Resistant Starch Content	26:49	Resistant Starch Content of Rice	26:57	Effects of Differences in Resistant Starch Content of Rice on Intestinal Microbial Composition.
34236836	3	33	theme	gene	523:526	arg1	families					528:535	predicted gene families	513:535	predicted gene families of glycosidases and functional pathways associated with carbohydrate and glycan metabolism	513:626	We confirmed that RS decreased the Firmicutes to Bacteroidetes ratio, increased SCFA production by higher Bacteroidaceae and S24-7 abundance, and enriched predicted gene families of glycosidases and functional pathways associated with carbohydrate and glycan metabolism.
34236836	2	34	dep	low	332:334	arg1	%					339:339	10%	337:339	10%	337:339	Three levels of RS from three varieties of rice were the major source of carbohydrates and fat levels were low (10%) and high (39%).
34236836	5	35	theme	RS	773:774	arg1	levels					776:781	increasing RS levels	762:781	increasing RS levels	762:781	Moreover, increasing RS levels resulted in a molecular ecological network with enhanced modularity and interspecific synergy, which is less sensitive to high fat intervention.
34236836	1	36	theme	fat	175:177	arg1	levels					179:184	fat levels	175:184	fat levels	175:184	The aim of this study was to evaluate the effects of resistant starch (RS) and fat levels on the gut microbiome in C57BL/6 mice.
34236836	5	37	theme	enhanced	831:838	arg1	modularity					840:849	enhanced modularity	831:849	enhanced modularity	831:849	Moreover, increasing RS levels resulted in a molecular ecological network with enhanced modularity and interspecific synergy, which is less sensitive to high fat intervention.
34236836	3	38	theme	pathways	568:575	arg1	families					528:535	predicted gene families	513:535	predicted gene families of glycosidases and functional pathways associated with carbohydrate and glycan metabolism	513:626	We confirmed that RS decreased the Firmicutes to Bacteroidetes ratio, increased SCFA production by higher Bacteroidaceae and S24-7 abundance, and enriched predicted gene families of glycosidases and functional pathways associated with carbohydrate and glycan metabolism.
34236836	6	39	theme	protective	1032:1041	arg1	effect					1043:1048	a protective effect	1030:1048	a protective effect against deleterious effects of an obesogenic diet	1030:1098	Overall, RS as low as 0.44% from cooked rice can modulate gut microbiome in mice, which correlated to a protective effect against deleterious effects of an obesogenic diet.
34236836	5	40	theme	fat	910:912	arg1	intervention					914:925	high fat intervention	905:925	high fat intervention	905:925	Moreover, increasing RS levels resulted in a molecular ecological network with enhanced modularity and interspecific synergy, which is less sensitive to high fat intervention.
34236836	4	41	theme	related	706:712	arg1	taxa					674:677	microbial taxa	664:677	microbial taxa	664:677	We also found correlations between microbial taxa and tissue gene expression related to carbohydrate and lipid metabolism.
34236836	1	42	theme	study	112:116	arg1	aim					100:102	The aim	96:102	The aim of this study	96:116	The aim of this study was to evaluate the effects of resistant starch (RS) and fat levels on the gut microbiome in C57BL/6 mice.
34236836	4	43	theme	microbial	664:672	arg1	taxa					674:677	microbial taxa	664:677	microbial taxa	664:677	We also found correlations between microbial taxa and tissue gene expression related to carbohydrate and lipid metabolism.
34236836	1	44	theme	levels	179:184	arg1	effects					138:144	the effects	134:144	the effects of resistant starch (RS) and fat levels on the gut microbiome in C57BL/6 mice	134:222	The aim of this study was to evaluate the effects of resistant starch (RS) and fat levels on the gut microbiome in C57BL/6 mice.
34236836	5	45	theme	high	905:908	arg1	intervention					914:925	high fat intervention	905:925	high fat intervention	905:925	Moreover, increasing RS levels resulted in a molecular ecological network with enhanced modularity and interspecific synergy, which is less sensitive to high fat intervention.
34236836	3	46	theme	predicted	513:521	arg1	families					528:535	predicted gene families	513:535	predicted gene families of glycosidases and functional pathways associated with carbohydrate and glycan metabolism	513:626	We confirmed that RS decreased the Firmicutes to Bacteroidetes ratio, increased SCFA production by higher Bacteroidaceae and S24-7 abundance, and enriched predicted gene families of glycosidases and functional pathways associated with carbohydrate and glycan metabolism.
34236836	5	47	theme	interspecific	855:867	arg1	synergy					869:875	interspecific synergy	855:875	interspecific synergy	855:875	Moreover, increasing RS levels resulted in a molecular ecological network with enhanced modularity and interspecific synergy, which is less sensitive to high fat intervention.
34236836	2	48	theme	rice	268:271	arg1	RS					241:242	RS	241:242	RS from three varieties of rice	241:271	Three levels of RS from three varieties of rice were the major source of carbohydrates and fat levels were low (10%) and high (39%).
34236836	3	49	theme	functional	557:566	arg1	pathways					568:575	functional pathways	557:575	functional pathways	557:575	We confirmed that RS decreased the Firmicutes to Bacteroidetes ratio, increased SCFA production by higher Bacteroidaceae and S24-7 abundance, and enriched predicted gene families of glycosidases and functional pathways associated with carbohydrate and glycan metabolism.
34236836	6	50	theme	diet	1095:1098	arg1	effects					1070:1076	deleterious effects	1058:1076	deleterious effects of an obesogenic diet	1058:1098	Overall, RS as low as 0.44% from cooked rice can modulate gut microbiome in mice, which correlated to a protective effect against deleterious effects of an obesogenic diet.
34236836	6	51	theme	deleterious	1058:1068	arg1	effects					1070:1076	deleterious effects	1058:1076	deleterious effects of an obesogenic diet	1058:1098	Overall, RS as low as 0.44% from cooked rice can modulate gut microbiome in mice, which correlated to a protective effect against deleterious effects of an obesogenic diet.
34236836	2	52	theme	carbohydrates	298:310	arg1	levels					231:236	Three levels	225:236	Three levels of RS from three varieties of rice	225:271	Three levels of RS from three varieties of rice were the major source of carbohydrates and fat levels were low (10%) and high (39%).
34236836	2	52	theme	carbohydrates	298:310	arg1	source					288:293	the major source	278:293	the major source of carbohydrates	278:310	Three levels of RS from three varieties of rice were the major source of carbohydrates and fat levels were low (10%) and high (39%).
34236836	2	53	from	levels	231:236	arg1	varieties					255:263	three varieties	249:263	three varieties	249:263	Three levels of RS from three varieties of rice were the major source of carbohydrates and fat levels were low (10%) and high (39%).
34236836	0	54	theme	Rice	54:57	arg1	Content					43:49	Resistant Starch Content	26:49	Resistant Starch Content of Rice	26:57	Effects of Differences in Resistant Starch Content of Rice on Intestinal Microbial Composition.
34236836	6	55	theme	obesogenic	1084:1093	arg1	diet					1095:1098	an obesogenic diet	1081:1098	an obesogenic diet	1081:1098	Overall, RS as low as 0.44% from cooked rice can modulate gut microbiome in mice, which correlated to a protective effect against deleterious effects of an obesogenic diet.
34236836	3	56	theme	glycan	610:615	arg1	metabolism					617:626	glycan metabolism	610:626	glycan metabolism	610:626	We confirmed that RS decreased the Firmicutes to Bacteroidetes ratio, increased SCFA production by higher Bacteroidaceae and S24-7 abundance, and enriched predicted gene families of glycosidases and functional pathways associated with carbohydrate and glycan metabolism.
34236836	5	57	theme	ecological	807:816	arg1	network					818:824	a molecular ecological network	795:824	a molecular ecological network	795:824	Moreover, increasing RS levels resulted in a molecular ecological network with enhanced modularity and interspecific synergy, which is less sensitive to high fat intervention.
34236836	5	57	theme	ecological	807:816	arg1	sensitive					892:900	sensitive	892:900	sensitive	892:900	Moreover, increasing RS levels resulted in a molecular ecological network with enhanced modularity and interspecific synergy, which is less sensitive to high fat intervention.
34236836	1	58	theme	gut	193:195	arg1	microbiome					197:206	the gut microbiome	189:206	the gut microbiome in C57BL/6 mice	189:222	The aim of this study was to evaluate the effects of resistant starch (RS) and fat levels on the gut microbiome in C57BL/6 mice.
32049502	5	0	theme	physical	806:813	arg1	properties					815:824	thermal and physical properties	794:824	thermal and physical properties of the composites	794:842	The degree of acetylation and amount of OCC were varied to establish their influence on thermal and physical properties of the composites.
32049502	0	1	with	Composites	30:39	arg1	Cellulose					77:85	Covalently Attached Carbonized Cellulose	46:85	Covalently Attached Carbonized Cellulose	46:85	Thermoplastic "All-Cellulose" Composites with Covalently Attached Carbonized Cellulose.
32049502	1	2	theme	cellulose	179:187	arg1	CA					198:199	CA	198:199	CA	198:199	Thermoplastic "all-cellulose" composites were synthesized by covalent functionalization of cellulose acetate (CA) with oxidized carbonized cellulose (OCC).
32049502	1	2	theme	cellulose	179:187	arg1	acetate					189:195	cellulose acetate	179:195	cellulose acetate (CA)	179:200	Thermoplastic "all-cellulose" composites were synthesized by covalent functionalization of cellulose acetate (CA) with oxidized carbonized cellulose (OCC).
32049502	7	3	theme	waste	1086:1090	arg1	tissues					1116:1122	paper tissues	1110:1122	paper tissues	1110:1122	In addition, it was shown that OCC-modified CA composites can be synthesized from waste products, such as paper tissues.
32049502	7	3	theme	waste	1086:1090	arg1	products					1092:1099	waste products	1086:1099	waste products	1086:1099	In addition, it was shown that OCC-modified CA composites can be synthesized from waste products, such as paper tissues.
32049502	4	4	theme	neat	614:617	arg1	CA					619:620	neat CA	614:620	neat CA	614:620	The synthesis of OCC-modified CA composites and neat CA were performed in the recyclable ionic liquid 1-allyl-3-methylimidazolium chloride.
32049502	6	5	theme	mechanical	961:970	arg1	properties					972:981	improved optical and mechanical properties	940:981	improved optical and mechanical properties	940:981	The OCC-modified CA composites displayed a notably enhanced film-forming ability, which led to improved optical and mechanical properties compared to neat CA.
32049502	1	6	theme	acetate	189:195	arg1	functionalization					158:174	covalent functionalization	149:174	covalent functionalization of cellulose acetate (CA) with oxidized carbonized cellulose (OCC)	149:241	Thermoplastic "all-cellulose" composites were synthesized by covalent functionalization of cellulose acetate (CA) with oxidized carbonized cellulose (OCC).
32049502	1	7	with	functionalization	158:174	arg1	OCC					238:240	OCC	238:240	OCC	238:240	Thermoplastic "all-cellulose" composites were synthesized by covalent functionalization of cellulose acetate (CA) with oxidized carbonized cellulose (OCC).
32049502	1	7	with	functionalization	158:174	arg1	cellulose					227:235	oxidized carbonized cellulose	207:235	oxidized carbonized cellulose (OCC)	207:241	Thermoplastic "all-cellulose" composites were synthesized by covalent functionalization of cellulose acetate (CA) with oxidized carbonized cellulose (OCC).
32049502	3	8	contain	had	408:410	arg2	morphology					418:427	plane morphology	412:427	plane morphology	412:427	The OCC were of micrometer-size, had plane morphology and contained a variety of oxygen functionalities, enabling transformation into acyl chlorinated OCC under moderate reaction conditions.
32049502	3	8	contain	had	408:410	arg1	OCC					379:381	The OCC	375:381	The OCC	375:381	The OCC were of micrometer-size, had plane morphology and contained a variety of oxygen functionalities, enabling transformation into acyl chlorinated OCC under moderate reaction conditions.
32049502	5	9	theme	composites	833:842	arg1	properties					815:824	thermal and physical properties	794:824	thermal and physical properties of the composites	794:842	The degree of acetylation and amount of OCC were varied to establish their influence on thermal and physical properties of the composites.
32049502	8	10	theme	transparent	1190:1200	arg1	composites					1243:1252	transparent and strong thermoplastic "all-cellulose" composites	1190:1252	transparent and strong thermoplastic "all-cellulose" composites with moderate flexibility	1190:1278	The OCC-modification was demonstrated to be a promising route to transparent and strong thermoplastic "all-cellulose" composites with moderate flexibility.
32049502	6	11	theme	optical	949:955	arg1	properties					972:981	improved optical and mechanical properties	940:981	improved optical and mechanical properties	940:981	The OCC-modified CA composites displayed a notably enhanced film-forming ability, which led to improved optical and mechanical properties compared to neat CA.
32049502	6	12	theme	OCC-modified	849:860	arg1	composites					865:874	The OCC-modified CA composites	845:874	The OCC-modified CA composites	845:874	The OCC-modified CA composites displayed a notably enhanced film-forming ability, which led to improved optical and mechanical properties compared to neat CA.
32049502	4	13	theme	CA	619:620	arg1	synthesis					570:578	The synthesis	566:578	The synthesis of OCC-modified CA composites and neat CA	566:620	The synthesis of OCC-modified CA composites and neat CA were performed in the recyclable ionic liquid 1-allyl-3-methylimidazolium chloride.
32049502	8	14	theme	thermoplastic	1213:1225	arg1	composites					1243:1252	transparent and strong thermoplastic "all-cellulose" composites	1190:1252	transparent and strong thermoplastic "all-cellulose" composites with moderate flexibility	1190:1278	The OCC-modification was demonstrated to be a promising route to transparent and strong thermoplastic "all-cellulose" composites with moderate flexibility.
32049502	6	15	theme	improved	940:947	arg1	properties					972:981	improved optical and mechanical properties	940:981	improved optical and mechanical properties	940:981	The OCC-modified CA composites displayed a notably enhanced film-forming ability, which led to improved optical and mechanical properties compared to neat CA.
32049502	4	16	theme	ionic	655:659	arg1	chloride					696:703	the recyclable ionic liquid 1-allyl-3-methylimidazolium chloride	640:703	the recyclable ionic liquid 1-allyl-3-methylimidazolium chloride	640:703	The synthesis of OCC-modified CA composites and neat CA were performed in the recyclable ionic liquid 1-allyl-3-methylimidazolium chloride.
32049502	2	17	theme	hydrothermal	293:304	arg1	carbonization					306:318	microwave-assisted hydrothermal carbonization	274:318	microwave-assisted hydrothermal carbonization (HTC) of cellulose followed by oxidation and dialysis	274:372	The OCC were manufactured via microwave-assisted hydrothermal carbonization (HTC) of cellulose followed by oxidation and dialysis.
32049502	2	17	theme	hydrothermal	293:304	arg1	HTC					321:323	HTC	321:323	HTC	321:323	The OCC were manufactured via microwave-assisted hydrothermal carbonization (HTC) of cellulose followed by oxidation and dialysis.
32049502	2	18	theme	microwave-assisted	274:291	arg1	carbonization					306:318	microwave-assisted hydrothermal carbonization	274:318	microwave-assisted hydrothermal carbonization (HTC) of cellulose followed by oxidation and dialysis	274:372	The OCC were manufactured via microwave-assisted hydrothermal carbonization (HTC) of cellulose followed by oxidation and dialysis.
32049502	2	18	theme	microwave-assisted	274:291	arg1	HTC					321:323	HTC	321:323	HTC	321:323	The OCC were manufactured via microwave-assisted hydrothermal carbonization (HTC) of cellulose followed by oxidation and dialysis.
32049502	8	19	theme	all-cellulose	1228:1240	arg1	composites					1243:1252	transparent and strong thermoplastic "all-cellulose" composites	1190:1252	transparent and strong thermoplastic "all-cellulose" composites with moderate flexibility	1190:1278	The OCC-modification was demonstrated to be a promising route to transparent and strong thermoplastic "all-cellulose" composites with moderate flexibility.
32049502	8	20	theme	promising	1171:1179	arg1	route					1181:1185	a promising route	1169:1185	a promising route to transparent and strong thermoplastic "all-cellulose" composites with moderate flexibility	1169:1278	The OCC-modification was demonstrated to be a promising route to transparent and strong thermoplastic "all-cellulose" composites with moderate flexibility.
32049502	8	20	theme	promising	1171:1179	arg1	OCC-modification					1129:1144	The OCC-modification	1125:1144	The OCC-modification	1125:1144	The OCC-modification was demonstrated to be a promising route to transparent and strong thermoplastic "all-cellulose" composites with moderate flexibility.
32049502	2	21	theme	cellulose	329:337	arg1	carbonization					306:318	microwave-assisted hydrothermal carbonization	274:318	microwave-assisted hydrothermal carbonization (HTC) of cellulose followed by oxidation and dialysis	274:372	The OCC were manufactured via microwave-assisted hydrothermal carbonization (HTC) of cellulose followed by oxidation and dialysis.
32049502	2	21	theme	cellulose	329:337	arg1	HTC					321:323	HTC	321:323	HTC	321:323	The OCC were manufactured via microwave-assisted hydrothermal carbonization (HTC) of cellulose followed by oxidation and dialysis.
32049502	0	22	theme	Thermoplastic	0:12	arg1	"					28:28	Thermoplastic "All-Cellulose"	0:28	Thermoplastic "All-Cellulose"	0:28	Thermoplastic "All-Cellulose" Composites with Covalently Attached Carbonized Cellulose.
32049502	4	23	theme	OCC-modified	583:594	arg1	composites					599:608	OCC-modified CA composites	583:608	OCC-modified CA composites	583:608	The synthesis of OCC-modified CA composites and neat CA were performed in the recyclable ionic liquid 1-allyl-3-methylimidazolium chloride.
32049502	1	24	theme	all-cellulose	103:115	arg1	composites					118:127	Thermoplastic "all-cellulose" composites	88:127	Thermoplastic "all-cellulose" composites	88:127	Thermoplastic "all-cellulose" composites were synthesized by covalent functionalization of cellulose acetate (CA) with oxidized carbonized cellulose (OCC).
32049502	5	25	theme	OCC	746:748	arg1	degree					710:715	The degree	706:715	The degree of acetylation and amount of OCC	706:748	The degree of acetylation and amount of OCC were varied to establish their influence on thermal and physical properties of the composites.
32049502	1	26	theme	"	116:116	arg1	composites					118:127	Thermoplastic "all-cellulose" composites	88:127	Thermoplastic "all-cellulose" composites	88:127	Thermoplastic "all-cellulose" composites were synthesized by covalent functionalization of cellulose acetate (CA) with oxidized carbonized cellulose (OCC).
32049502	1	27	theme	oxidized	207:214	arg1	OCC					238:240	OCC	238:240	OCC	238:240	Thermoplastic "all-cellulose" composites were synthesized by covalent functionalization of cellulose acetate (CA) with oxidized carbonized cellulose (OCC).
32049502	1	27	theme	oxidized	207:214	arg1	cellulose					227:235	oxidized carbonized cellulose	207:235	oxidized carbonized cellulose (OCC)	207:241	Thermoplastic "all-cellulose" composites were synthesized by covalent functionalization of cellulose acetate (CA) with oxidized carbonized cellulose (OCC).
32049502	0	28	theme	All-Cellulose	15:27	arg1	"					28:28	Thermoplastic "All-Cellulose"	0:28	Thermoplastic "All-Cellulose"	0:28	Thermoplastic "All-Cellulose" Composites with Covalently Attached Carbonized Cellulose.
32049502	4	29	theme	composites	599:608	arg1	synthesis					570:578	The synthesis	566:578	The synthesis of OCC-modified CA composites and neat CA	566:620	The synthesis of OCC-modified CA composites and neat CA were performed in the recyclable ionic liquid 1-allyl-3-methylimidazolium chloride.
32049502	7	30	theme	paper	1110:1114	arg1	tissues					1116:1122	paper tissues	1110:1122	paper tissues	1110:1122	In addition, it was shown that OCC-modified CA composites can be synthesized from waste products, such as paper tissues.
32049502	5	31	from	influence	781:789	arg1	properties					815:824	thermal and physical properties	794:824	thermal and physical properties of the composites	794:842	The degree of acetylation and amount of OCC were varied to establish their influence on thermal and physical properties of the composites.
32049502	1	32	theme	carbonized	216:225	arg1	OCC					238:240	OCC	238:240	OCC	238:240	Thermoplastic "all-cellulose" composites were synthesized by covalent functionalization of cellulose acetate (CA) with oxidized carbonized cellulose (OCC).
32049502	1	32	theme	carbonized	216:225	arg1	cellulose					227:235	oxidized carbonized cellulose	207:235	oxidized carbonized cellulose (OCC)	207:241	Thermoplastic "all-cellulose" composites were synthesized by covalent functionalization of cellulose acetate (CA) with oxidized carbonized cellulose (OCC).
32049502	8	33	theme	moderate	1259:1266	arg1	flexibility					1268:1278	moderate flexibility	1259:1278	moderate flexibility	1259:1278	The OCC-modification was demonstrated to be a promising route to transparent and strong thermoplastic "all-cellulose" composites with moderate flexibility.
32049502	4	34	theme	CA	596:597	arg1	composites					599:608	OCC-modified CA composites	583:608	OCC-modified CA composites	583:608	The synthesis of OCC-modified CA composites and neat CA were performed in the recyclable ionic liquid 1-allyl-3-methylimidazolium chloride.
32049502	3	35	theme	oxygen	456:461	arg1	functionalities					463:477	oxygen functionalities	456:477	oxygen functionalities	456:477	The OCC were of micrometer-size, had plane morphology and contained a variety of oxygen functionalities, enabling transformation into acyl chlorinated OCC under moderate reaction conditions.
32049502	8	36	with	composites	1243:1252	arg1	flexibility					1268:1278	moderate flexibility	1259:1278	moderate flexibility	1259:1278	The OCC-modification was demonstrated to be a promising route to transparent and strong thermoplastic "all-cellulose" composites with moderate flexibility.
32049502	3	37	theme	functionalities	463:477	arg1	variety					445:451	a variety	443:451	a variety of oxygen functionalities	443:477	The OCC were of micrometer-size, had plane morphology and contained a variety of oxygen functionalities, enabling transformation into acyl chlorinated OCC under moderate reaction conditions.
32049502	3	37	theme	functionalities	463:477	arg1	functionalities					463:477	oxygen functionalities	456:477	oxygen functionalities	456:477	The OCC were of micrometer-size, had plane morphology and contained a variety of oxygen functionalities, enabling transformation into acyl chlorinated OCC under moderate reaction conditions.
32049502	0	38	theme	Attached	57:64	arg1	Cellulose					77:85	Covalently Attached Carbonized Cellulose	46:85	Covalently Attached Carbonized Cellulose	46:85	Thermoplastic "All-Cellulose" Composites with Covalently Attached Carbonized Cellulose.
32049502	5	39	theme	acetylation	720:730	arg1	degree					710:715	The degree	706:715	The degree of acetylation and amount of OCC	706:748	The degree of acetylation and amount of OCC were varied to establish their influence on thermal and physical properties of the composites.
32049502	6	40	theme	film-forming	905:916	arg1	ability					918:924	a notably enhanced film-forming ability	886:924	a notably enhanced film-forming ability	886:924	The OCC-modified CA composites displayed a notably enhanced film-forming ability, which led to improved optical and mechanical properties compared to neat CA.
32049502	3	41	theme	acyl	509:512	arg1	OCC					526:528	acyl chlorinated OCC	509:528	acyl chlorinated OCC	509:528	The OCC were of micrometer-size, had plane morphology and contained a variety of oxygen functionalities, enabling transformation into acyl chlorinated OCC under moderate reaction conditions.
32049502	3	42	theme	plane	412:416	arg1	morphology					418:427	plane morphology	412:427	plane morphology	412:427	The OCC were of micrometer-size, had plane morphology and contained a variety of oxygen functionalities, enabling transformation into acyl chlorinated OCC under moderate reaction conditions.
32049502	6	43	theme	neat	995:998	arg1	CA					1000:1001	neat CA	995:1001	neat CA	995:1001	The OCC-modified CA composites displayed a notably enhanced film-forming ability, which led to improved optical and mechanical properties compared to neat CA.
32049502	6	44	theme	enhanced	896:903	arg1	ability					918:924	a notably enhanced film-forming ability	886:924	a notably enhanced film-forming ability	886:924	The OCC-modified CA composites displayed a notably enhanced film-forming ability, which led to improved optical and mechanical properties compared to neat CA.
32049502	7	45	theme	CA	1048:1049	arg1	composites					1051:1060	OCC-modified CA composites	1035:1060	OCC-modified CA composites	1035:1060	In addition, it was shown that OCC-modified CA composites can be synthesized from waste products, such as paper tissues.
32049502	3	46	theme	chlorinated	514:524	arg1	OCC					526:528	acyl chlorinated OCC	509:528	acyl chlorinated OCC	509:528	The OCC were of micrometer-size, had plane morphology and contained a variety of oxygen functionalities, enabling transformation into acyl chlorinated OCC under moderate reaction conditions.
32049502	7	47	theme	OCC-modified	1035:1046	arg1	composites					1051:1060	OCC-modified CA composites	1035:1060	OCC-modified CA composites	1035:1060	In addition, it was shown that OCC-modified CA composites can be synthesized from waste products, such as paper tissues.
32049502	5	48	theme	amount	736:741	arg1	degree					710:715	The degree	706:715	The degree of acetylation and amount of OCC	706:748	The degree of acetylation and amount of OCC were varied to establish their influence on thermal and physical properties of the composites.
32049502	3	49	contain	contained	433:441	arg2	functionalities					463:477	oxygen functionalities	456:477	oxygen functionalities	456:477	The OCC were of micrometer-size, had plane morphology and contained a variety of oxygen functionalities, enabling transformation into acyl chlorinated OCC under moderate reaction conditions.
32049502	3	49	contain	contained	433:441	arg2	variety					445:451	a variety	443:451	a variety of oxygen functionalities	443:477	The OCC were of micrometer-size, had plane morphology and contained a variety of oxygen functionalities, enabling transformation into acyl chlorinated OCC under moderate reaction conditions.
32049502	3	49	contain	contained	433:441	arg1	OCC					379:381	The OCC	375:381	The OCC	375:381	The OCC were of micrometer-size, had plane morphology and contained a variety of oxygen functionalities, enabling transformation into acyl chlorinated OCC under moderate reaction conditions.
32049502	6	50	theme	CA	862:863	arg1	composites					865:874	The OCC-modified CA composites	845:874	The OCC-modified CA composites	845:874	The OCC-modified CA composites displayed a notably enhanced film-forming ability, which led to improved optical and mechanical properties compared to neat CA.
32049502	1	51	theme	Thermoplastic	88:100	arg1	composites					118:127	Thermoplastic "all-cellulose" composites	88:127	Thermoplastic "all-cellulose" composites	88:127	Thermoplastic "all-cellulose" composites were synthesized by covalent functionalization of cellulose acetate (CA) with oxidized carbonized cellulose (OCC).
32049502	1	52	theme	covalent	149:156	arg1	functionalization					158:174	covalent functionalization	149:174	covalent functionalization of cellulose acetate (CA) with oxidized carbonized cellulose (OCC)	149:241	Thermoplastic "all-cellulose" composites were synthesized by covalent functionalization of cellulose acetate (CA) with oxidized carbonized cellulose (OCC).
32049502	5	53	theme	thermal	794:800	arg1	properties					815:824	thermal and physical properties	794:824	thermal and physical properties of the composites	794:842	The degree of acetylation and amount of OCC were varied to establish their influence on thermal and physical properties of the composites.
32049502	4	54	theme	1-allyl-3-methylimidazolium	668:694	arg1	chloride					696:703	the recyclable ionic liquid 1-allyl-3-methylimidazolium chloride	640:703	the recyclable ionic liquid 1-allyl-3-methylimidazolium chloride	640:703	The synthesis of OCC-modified CA composites and neat CA were performed in the recyclable ionic liquid 1-allyl-3-methylimidazolium chloride.
32049502	4	55	theme	liquid	661:666	arg1	chloride					696:703	the recyclable ionic liquid 1-allyl-3-methylimidazolium chloride	640:703	the recyclable ionic liquid 1-allyl-3-methylimidazolium chloride	640:703	The synthesis of OCC-modified CA composites and neat CA were performed in the recyclable ionic liquid 1-allyl-3-methylimidazolium chloride.
32049502	3	56	theme	moderate	536:543	arg1	conditions					554:563	moderate reaction conditions	536:563	moderate reaction conditions	536:563	The OCC were of micrometer-size, had plane morphology and contained a variety of oxygen functionalities, enabling transformation into acyl chlorinated OCC under moderate reaction conditions.
32049502	4	57	theme	recyclable	644:653	arg1	chloride					696:703	the recyclable ionic liquid 1-allyl-3-methylimidazolium chloride	640:703	the recyclable ionic liquid 1-allyl-3-methylimidazolium chloride	640:703	The synthesis of OCC-modified CA composites and neat CA were performed in the recyclable ionic liquid 1-allyl-3-methylimidazolium chloride.
32049502	0	58	theme	Carbonized	66:75	arg1	Cellulose					77:85	Covalently Attached Carbonized Cellulose	46:85	Covalently Attached Carbonized Cellulose	46:85	Thermoplastic "All-Cellulose" Composites with Covalently Attached Carbonized Cellulose.
32049502	8	59	theme	"	1241:1241	arg1	composites					1243:1252	transparent and strong thermoplastic "all-cellulose" composites	1190:1252	transparent and strong thermoplastic "all-cellulose" composites with moderate flexibility	1190:1278	The OCC-modification was demonstrated to be a promising route to transparent and strong thermoplastic "all-cellulose" composites with moderate flexibility.
32049502	3	60	theme	reaction	545:552	arg1	conditions					554:563	moderate reaction conditions	536:563	moderate reaction conditions	536:563	The OCC were of micrometer-size, had plane morphology and contained a variety of oxygen functionalities, enabling transformation into acyl chlorinated OCC under moderate reaction conditions.
32645493	7	0	theme	chitosan/CMC	1227:1238	arg1	film					1240:1243	the chitosan/CMC film	1223:1243	the chitosan/CMC film	1223:1243	Both GO and rGO reduced the relaxation temperatures of the chitosan/CMC film with 20 wt% glycerol added, resulting in increased biopolymer chain mobility.
32645493	3	1	theme	polyelectrolyte	704:718	arg1	PEC					734:736	PEC	734:736	PEC	734:736	For the chitosan/CMC matrix, GO or rGO was likely to disrupt polyelectrolyte complexation (PEC) between the two biopolymers, leading to weakened mechanical properties and increased surface hydrophilicity.
32645493	3	1	theme	polyelectrolyte	704:718	arg1	complexation					720:731	polyelectrolyte complexation	704:731	polyelectrolyte complexation (PEC)	704:737	For the chitosan/CMC matrix, GO or rGO was likely to disrupt polyelectrolyte complexation (PEC) between the two biopolymers, leading to weakened mechanical properties and increased surface hydrophilicity.
32645493	2	2	theme	CMC	631:633	arg1	blend					636:640	a chitosan/carboxymethyl cellulose (CMC) blend	595:640	a chitosan/carboxymethyl cellulose (CMC) blend	595:640	This study shows that glycerol plasticisation and the addition of graphene oxide (GO) or reduced graphene oxide (rGO) altered the properties of chitosan and a chitosan/carboxymethyl cellulose (CMC) blend.
32645493	6	3	theme	chitosan	1121:1128	arg1	film					1130:1133	the chitosan film	1117:1133	the chitosan film	1117:1133	Specifically, GO/rGO notably increased the toughness of the chitosan film on inclusion of 40 wt% glycerol.
32645493	7	4	theme	film	1240:1243	arg1	temperatures					1207:1218	the relaxation temperatures	1192:1218	the relaxation temperatures of the chitosan/CMC film	1192:1243	Both GO and rGO reduced the relaxation temperatures of the chitosan/CMC film with 20 wt% glycerol added, resulting in increased biopolymer chain mobility.
32645493	1	5	theme	properties	370:379	arg1	properties					370:379	their properties	364:379	their properties	364:379	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	1	5	theme	properties	370:379	arg1	many					356:359	many	356:359	many	356:359	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	2	6	dep	oxide	513:517	arg1	GO					520:521	GO	520:521	GO	520:521	This study shows that glycerol plasticisation and the addition of graphene oxide (GO) or reduced graphene oxide (rGO) altered the properties of chitosan and a chitosan/carboxymethyl cellulose (CMC) blend.
32645493	9	7	theme	complex	1429:1435	arg1	interactions					1437:1448	complex interactions	1429:1448	complex interactions in multiphasic biopolymer composite systems	1429:1492	Thus, this work describes how complex interactions in multiphasic biopolymer composite systems influence structure and properties.
32645493	9	8	from	interactions	1437:1448	arg1	systems					1486:1492	multiphasic biopolymer composite systems	1453:1492	multiphasic biopolymer composite systems	1453:1492	Thus, this work describes how complex interactions in multiphasic biopolymer composite systems influence structure and properties.
32645493	2	9	theme	cellulose	620:628	arg1	blend					636:640	a chitosan/carboxymethyl cellulose (CMC) blend	595:640	a chitosan/carboxymethyl cellulose (CMC) blend	595:640	This study shows that glycerol plasticisation and the addition of graphene oxide (GO) or reduced graphene oxide (rGO) altered the properties of chitosan and a chitosan/carboxymethyl cellulose (CMC) blend.
32645493	5	10	theme	synergistic	985:995	arg1	effects					997:1003	some synergistic effects	980:1003	some synergistic effects from a combination of glycerol and GO/rGO	980:1045	Moreover, some synergistic effects from a combination of glycerol and GO/rGO were evident.
32645493	3	11	theme	surface	824:830	arg1	hydrophilicity					832:845	increased surface hydrophilicity	814:845	increased surface hydrophilicity	814:845	For the chitosan/CMC matrix, GO or rGO was likely to disrupt polyelectrolyte complexation (PEC) between the two biopolymers, leading to weakened mechanical properties and increased surface hydrophilicity.
32645493	2	12	theme	chitosan/carboxymethyl	597:618	arg1	blend					636:640	a chitosan/carboxymethyl cellulose (CMC) blend	595:640	a chitosan/carboxymethyl cellulose (CMC) blend	595:640	This study shows that glycerol plasticisation and the addition of graphene oxide (GO) or reduced graphene oxide (rGO) altered the properties of chitosan and a chitosan/carboxymethyl cellulose (CMC) blend.
32645493	7	13	theme	%	1255:1255	arg1	glycerol					1257:1264	20 wt% glycerol	1250:1264	20 wt% glycerol added	1250:1270	Both GO and rGO reduced the relaxation temperatures of the chitosan/CMC film with 20 wt% glycerol added, resulting in increased biopolymer chain mobility.
32645493	1	14	theme	chemical	299:306	arg1	versatility					308:318	chemical versatility	299:318	chemical versatility	299:318	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	2	15	theme	oxide	544:548	arg1	plasticisation					469:482	glycerol plasticisation	460:482	glycerol plasticisation	460:482	This study shows that glycerol plasticisation and the addition of graphene oxide (GO) or reduced graphene oxide (rGO) altered the properties of chitosan and a chitosan/carboxymethyl cellulose (CMC) blend.
32645493	2	15	theme	oxide	544:548	arg1	addition					492:499	the addition	488:499	the addition of graphene oxide (GO) or reduced graphene oxide (rGO)	488:554	This study shows that glycerol plasticisation and the addition of graphene oxide (GO) or reduced graphene oxide (rGO) altered the properties of chitosan and a chitosan/carboxymethyl cellulose (CMC) blend.
32645493	1	16	theme	further	397:403	arg1	tailoring					405:413	further tailoring	397:413	further tailoring for specific purposes	397:435	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	6	17	from	toughness	1104:1112	arg1	inclusion					1138:1146	inclusion	1138:1146	inclusion of 40 wt% glycerol	1138:1165	Specifically, GO/rGO notably increased the toughness of the chitosan film on inclusion of 40 wt% glycerol.
32645493	9	18	theme	multiphasic	1453:1463	arg1	systems					1486:1492	multiphasic biopolymer composite systems	1453:1492	multiphasic biopolymer composite systems	1453:1492	Thus, this work describes how complex interactions in multiphasic biopolymer composite systems influence structure and properties.
32645493	2	19	theme	graphene	535:542	arg1	oxide					544:548	reduced graphene oxide	527:548	reduced graphene oxide (rGO)	527:554	This study shows that glycerol plasticisation and the addition of graphene oxide (GO) or reduced graphene oxide (rGO) altered the properties of chitosan and a chitosan/carboxymethyl cellulose (CMC) blend.
32645493	2	19	theme	graphene	535:542	arg1	rGO					551:553	rGO	551:553	rGO	551:553	This study shows that glycerol plasticisation and the addition of graphene oxide (GO) or reduced graphene oxide (rGO) altered the properties of chitosan and a chitosan/carboxymethyl cellulose (CMC) blend.
32645493	5	20	from	combination	1012:1022	arg1	effects					997:1003	some synergistic effects	980:1003	some synergistic effects from a combination of glycerol and GO/rGO	980:1045	Moreover, some synergistic effects from a combination of glycerol and GO/rGO were evident.
32645493	1	21	theme	much	197:200	arg1	interest					202:209	much interest	197:209	much interest	197:209	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	2	22	theme	reduced	527:533	arg1	oxide					544:548	reduced graphene oxide	527:548	reduced graphene oxide (rGO)	527:554	This study shows that glycerol plasticisation and the addition of graphene oxide (GO) or reduced graphene oxide (rGO) altered the properties of chitosan and a chitosan/carboxymethyl cellulose (CMC) blend.
32645493	2	22	theme	reduced	527:533	arg1	rGO					551:553	rGO	551:553	rGO	551:553	This study shows that glycerol plasticisation and the addition of graphene oxide (GO) or reduced graphene oxide (rGO) altered the properties of chitosan and a chitosan/carboxymethyl cellulose (CMC) blend.
32645493	9	23	theme	composite	1476:1484	arg1	systems					1486:1492	multiphasic biopolymer composite systems	1453:1492	multiphasic biopolymer composite systems	1453:1492	Thus, this work describes how complex interactions in multiphasic biopolymer composite systems influence structure and properties.
32645493	0	24	theme	Glycerol	0:7	arg1	plasticisation					9:22	Glycerol plasticisation	0:22	Glycerol plasticisation of chitosan/carboxymethyl cellulose composites: Role of interactions in determining structure and properties.	0:132	Glycerol plasticisation of chitosan/carboxymethyl cellulose composites: Role of interactions in determining structure and properties.
32645493	0	25	theme	chitosan/carboxymethyl	27:48	arg1	composites					60:69	chitosan/carboxymethyl cellulose composites	27:69	chitosan/carboxymethyl cellulose composites	27:69	Glycerol plasticisation of chitosan/carboxymethyl cellulose composites: Role of interactions in determining structure and properties.
32645493	5	26	theme	glycerol	1027:1034	arg1	combination					1012:1022	a combination	1010:1022	a combination of glycerol and GO/rGO	1010:1045	Moreover, some synergistic effects from a combination of glycerol and GO/rGO were evident.
32645493	4	27	theme	reduced	938:944	arg1	hydrophilicity					954:967	reduced surface hydrophilicity	938:967	reduced surface hydrophilicity	938:967	Conversely, glycerol assisted PEC by increasing the biopolymer chain mobility, leading to reduced surface hydrophilicity.
32645493	2	28	theme	glycerol	460:467	arg1	plasticisation					469:482	glycerol plasticisation	460:482	glycerol plasticisation	460:482	This study shows that glycerol plasticisation and the addition of graphene oxide (GO) or reduced graphene oxide (rGO) altered the properties of chitosan and a chitosan/carboxymethyl cellulose (CMC) blend.
32645493	1	29	theme	natural	324:330	arg1	functionality					332:344	natural functionality	324:344	natural functionality	324:344	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	1	30	theme	specific	419:426	arg1	purposes					428:435	specific purposes	419:435	specific purposes	419:435	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	6	31	theme	glycerol	1158:1165	arg1	inclusion					1138:1146	inclusion	1138:1146	inclusion of 40 wt% glycerol	1138:1165	Specifically, GO/rGO notably increased the toughness of the chitosan film on inclusion of 40 wt% glycerol.
32645493	0	32	theme	composites	60:69	arg1	plasticisation					9:22	Glycerol plasticisation	0:22	Glycerol plasticisation of chitosan/carboxymethyl cellulose composites: Role of interactions in determining structure and properties.	0:132	Glycerol plasticisation of chitosan/carboxymethyl cellulose composites: Role of interactions in determining structure and properties.
32645493	6	33	theme	40 wt	1151:1155	arg1	glycerol					1158:1165	40 wt% glycerol	1151:1165	40 wt% glycerol	1151:1165	Specifically, GO/rGO notably increased the toughness of the chitosan film on inclusion of 40 wt% glycerol.
32645493	7	34	theme	chain	1307:1311	arg1	mobility					1313:1320	increased biopolymer chain mobility	1286:1320	increased biopolymer chain mobility	1286:1320	Both GO and rGO reduced the relaxation temperatures of the chitosan/CMC film with 20 wt% glycerol added, resulting in increased biopolymer chain mobility.
32645493	5	35	theme	GO/rGO	1040:1045	arg1	combination					1012:1022	a combination	1010:1022	a combination of glycerol and GO/rGO	1010:1045	Moreover, some synergistic effects from a combination of glycerol and GO/rGO were evident.
32645493	1	36	theme	many	224:227	arg1	biocompatibility					281:296	biocompatibility	281:296	biocompatibility	281:296	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	1	36	theme	many	224:227	arg1	characteristics					239:253	many appealing characteristics	224:253	many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality	224:344	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	1	36	theme	many	224:227	arg1	biodegradability					263:278	biodegradability	263:278	biodegradability	263:278	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	1	36	theme	many	224:227	arg1	versatility					308:318	chemical versatility	299:318	chemical versatility	299:318	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	1	36	theme	many	224:227	arg1	functionality					332:344	natural functionality	324:344	natural functionality	324:344	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	0	37	theme	cellulose	50:58	arg1	composites					60:69	chitosan/carboxymethyl cellulose composites	27:69	chitosan/carboxymethyl cellulose composites	27:69	Glycerol plasticisation of chitosan/carboxymethyl cellulose composites: Role of interactions in determining structure and properties.
32645493	2	38	theme	oxide	513:517	arg1	plasticisation					469:482	glycerol plasticisation	460:482	glycerol plasticisation	460:482	This study shows that glycerol plasticisation and the addition of graphene oxide (GO) or reduced graphene oxide (rGO) altered the properties of chitosan and a chitosan/carboxymethyl cellulose (CMC) blend.
32645493	2	38	theme	oxide	513:517	arg1	addition					492:499	the addition	488:499	the addition of graphene oxide (GO) or reduced graphene oxide (rGO)	488:554	This study shows that glycerol plasticisation and the addition of graphene oxide (GO) or reduced graphene oxide (rGO) altered the properties of chitosan and a chitosan/carboxymethyl cellulose (CMC) blend.
32645493	1	39	theme	appealing	229:237	arg1	biocompatibility					281:296	biocompatibility	281:296	biocompatibility	281:296	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	1	39	theme	appealing	229:237	arg1	characteristics					239:253	many appealing characteristics	224:253	many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality	224:344	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	1	39	theme	appealing	229:237	arg1	biodegradability					263:278	biodegradability	263:278	biodegradability	263:278	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	1	39	theme	appealing	229:237	arg1	versatility					308:318	chemical versatility	299:318	chemical versatility	299:318	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	1	39	theme	appealing	229:237	arg1	functionality					332:344	natural functionality	324:344	natural functionality	324:344	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	2	40	theme	graphene	504:511	arg1	oxide					513:517	graphene oxide	504:517	graphene oxide (GO)	504:522	This study shows that glycerol plasticisation and the addition of graphene oxide (GO) or reduced graphene oxide (rGO) altered the properties of chitosan and a chitosan/carboxymethyl cellulose (CMC) blend.
32645493	3	41	theme	weakened	779:786	arg1	properties					799:808	weakened mechanical properties	779:808	weakened mechanical properties	779:808	For the chitosan/CMC matrix, GO or rGO was likely to disrupt polyelectrolyte complexation (PEC) between the two biopolymers, leading to weakened mechanical properties and increased surface hydrophilicity.
32645493	3	42	theme	chitosan/CMC	651:662	arg1	matrix					664:669	the chitosan/CMC matrix	647:669	the chitosan/CMC matrix	647:669	For the chitosan/CMC matrix, GO or rGO was likely to disrupt polyelectrolyte complexation (PEC) between the two biopolymers, leading to weakened mechanical properties and increased surface hydrophilicity.
32645493	6	43	theme	%	1156:1156	arg1	glycerol					1158:1165	40 wt% glycerol	1151:1165	40 wt% glycerol	1151:1165	Specifically, GO/rGO notably increased the toughness of the chitosan film on inclusion of 40 wt% glycerol.
32645493	4	44	theme	surface	946:952	arg1	hydrophilicity					954:967	reduced surface hydrophilicity	938:967	reduced surface hydrophilicity	938:967	Conversely, glycerol assisted PEC by increasing the biopolymer chain mobility, leading to reduced surface hydrophilicity.
32645493	4	45	theme	chain	911:915	arg1	mobility					917:924	the biopolymer chain mobility	896:924	the biopolymer chain mobility	896:924	Conversely, glycerol assisted PEC by increasing the biopolymer chain mobility, leading to reduced surface hydrophilicity.
32645493	7	46	theme	increased	1286:1294	arg1	mobility					1313:1320	increased biopolymer chain mobility	1286:1320	increased biopolymer chain mobility	1286:1320	Both GO and rGO reduced the relaxation temperatures of the chitosan/CMC film with 20 wt% glycerol added, resulting in increased biopolymer chain mobility.
32645493	4	47	theme	biopolymer	900:909	arg1	mobility					917:924	the biopolymer chain mobility	896:924	the biopolymer chain mobility	896:924	Conversely, glycerol assisted PEC by increasing the biopolymer chain mobility, leading to reduced surface hydrophilicity.
32645493	2	48	theme	chitosan	582:589	arg1	blend					636:640	a chitosan/carboxymethyl cellulose (CMC) blend	595:640	a chitosan/carboxymethyl cellulose (CMC) blend	595:640	This study shows that glycerol plasticisation and the addition of graphene oxide (GO) or reduced graphene oxide (rGO) altered the properties of chitosan and a chitosan/carboxymethyl cellulose (CMC) blend.
32645493	2	48	theme	chitosan	582:589	arg1	properties					568:577	the properties	564:577	the properties of chitosan	564:589	This study shows that glycerol plasticisation and the addition of graphene oxide (GO) or reduced graphene oxide (rGO) altered the properties of chitosan and a chitosan/carboxymethyl cellulose (CMC) blend.
32645493	1	49	contain	have	219:222	arg2	versatility					308:318	chemical versatility	299:318	chemical versatility	299:318	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	1	49	contain	have	219:222	arg2	biocompatibility					281:296	biocompatibility	281:296	biocompatibility	281:296	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	1	49	contain	have	219:222	arg2	functionality					332:344	natural functionality	324:344	natural functionality	324:344	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	1	49	contain	have	219:222	arg1	they					214:217	they	214:217	they	214:217	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	1	49	contain	have	219:222	arg2	characteristics					239:253	many appealing characteristics	224:253	many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality	224:344	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	1	49	contain	have	219:222	arg2	biodegradability					263:278	biodegradability	263:278	biodegradability	263:278	Biopolymers such as chitosan and cellulose continue to attract much interest as they have many appealing characteristics such as biodegradability, biocompatibility, chemical versatility and natural functionality; however, many of their properties usually require further tailoring for specific purposes.
32645493	8	50	theme	relative	1367:1374	arg1	permittivity					1376:1387	high relative permittivity	1362:1387	high relative permittivity (54-387)	1362:1396	Moreover, the bionanocomposites showed high relative permittivity (54-387).
32645493	8	50	theme	relative	1367:1374	arg1	54-387					1390:1395	54-387	1390:1395	54-387	1390:1395	Moreover, the bionanocomposites showed high relative permittivity (54-387).
32645493	8	51	theme	high	1362:1365	arg1	permittivity					1376:1387	high relative permittivity	1362:1387	high relative permittivity (54-387)	1362:1396	Moreover, the bionanocomposites showed high relative permittivity (54-387).
32645493	8	51	theme	high	1362:1365	arg1	54-387					1390:1395	54-387	1390:1395	54-387	1390:1395	Moreover, the bionanocomposites showed high relative permittivity (54-387).
32645493	3	52	theme	increased	814:822	arg1	hydrophilicity					832:845	increased surface hydrophilicity	814:845	increased surface hydrophilicity	814:845	For the chitosan/CMC matrix, GO or rGO was likely to disrupt polyelectrolyte complexation (PEC) between the two biopolymers, leading to weakened mechanical properties and increased surface hydrophilicity.
32645493	0	53	theme	interactions	80:91	arg1	Role					72:75	Role	72:75	Glycerol plasticisation of chitosan/carboxymethyl cellulose composites: Role of interactions in determining structure and properties.	0:132	Glycerol plasticisation of chitosan/carboxymethyl cellulose composites: Role of interactions in determining structure and properties.
32645493	0	54	dep	plasticisation	9:22	arg1	Role					72:75	Role	72:75	Glycerol plasticisation of chitosan/carboxymethyl cellulose composites: Role of interactions in determining structure and properties.	0:132	Glycerol plasticisation of chitosan/carboxymethyl cellulose composites: Role of interactions in determining structure and properties.
32645493	9	55	theme	biopolymer	1465:1474	arg1	systems					1486:1492	multiphasic biopolymer composite systems	1453:1492	multiphasic biopolymer composite systems	1453:1492	Thus, this work describes how complex interactions in multiphasic biopolymer composite systems influence structure and properties.
32645493	3	56	theme	mechanical	788:797	arg1	properties					799:808	weakened mechanical properties	779:808	weakened mechanical properties	779:808	For the chitosan/CMC matrix, GO or rGO was likely to disrupt polyelectrolyte complexation (PEC) between the two biopolymers, leading to weakened mechanical properties and increased surface hydrophilicity.
32645493	7	57	theme	20 wt	1250:1254	arg1	glycerol					1257:1264	20 wt% glycerol	1250:1264	20 wt% glycerol added	1250:1270	Both GO and rGO reduced the relaxation temperatures of the chitosan/CMC film with 20 wt% glycerol added, resulting in increased biopolymer chain mobility.
32645493	7	58	theme	relaxation	1196:1205	arg1	temperatures					1207:1218	the relaxation temperatures	1192:1218	the relaxation temperatures of the chitosan/CMC film	1192:1243	Both GO and rGO reduced the relaxation temperatures of the chitosan/CMC film with 20 wt% glycerol added, resulting in increased biopolymer chain mobility.
32645493	7	59	theme	biopolymer	1296:1305	arg1	mobility					1313:1320	increased biopolymer chain mobility	1286:1320	increased biopolymer chain mobility	1286:1320	Both GO and rGO reduced the relaxation temperatures of the chitosan/CMC film with 20 wt% glycerol added, resulting in increased biopolymer chain mobility.
32645493	6	60	theme	film	1130:1133	arg1	toughness					1104:1112	the toughness	1100:1112	the toughness of the chitosan film on inclusion of 40 wt% glycerol	1100:1165	Specifically, GO/rGO notably increased the toughness of the chitosan film on inclusion of 40 wt% glycerol.
34390795	8	0	theme	various	1173:1179	arg1	assays					1190:1195	various in vitro assays	1173:1195	various in vitro assays	1173:1195	Further, the immunostimulatory activity of polysaccharides was tested on murine macrophage cell line RAW264.7 by various in vitro assays.
34390795	4	1	theme	work	562:565	arg1	objective					544:552	The main objective	535:552	The main objective of this work	535:565	AIM OF THE STUDY The main objective of this work was to study the composition and structural properties of polysaccharides (TPP) from Taraxacum platycarpum Dahlst.
34390795	6	2	theme	hot	819:821	arg1	extraction					829:838	hot water extraction	819:838	hot water extraction	819:838	MATERIALS AND METHODS TPP was extracted from T. platycarpum using hot water extraction, ethanol precipitation method and its fractionated using DEAE-Sepharose fast flow column.
34390795	10	3	theme	9.73	1583:1586	arg1	%					1587:1587	9.73%	1583:1587	9.73%	1583:1587	RESULTS We obtained three fractions namely TPP-1, TPP-2, and TPP-3 which mainly consisted of carbohydrates (75.55, 52.71, and 48.41%), sulfate (8.42, 15.19, and 27.67%), uronic acid (1.27, 6.56, and 4.39%), and protein (8.15, 24.85, and 9.73%).
34390795	12	4	theme	NF-κB	1849:1853	arg1	pathways					1855:1862	NF-κB pathways	1849:1862	NF-κB pathways	1849:1862	The polysaccharides activate the RAW264.7 cell to produce a significant amount of NO and upregulate the various mRNA expression by the activation of MAPK and NF-κB pathways via TLR4, TLR2, and CR3 receptors.
34390795	6	5	theme	flow	917:920	arg1	column					922:927	DEAE-Sepharose fast flow column	897:927	DEAE-Sepharose fast flow column	897:927	MATERIALS AND METHODS TPP was extracted from T. platycarpum using hot water extraction, ethanol precipitation method and its fractionated using DEAE-Sepharose fast flow column.
34390795	6	6	theme	DEAE-Sepharose	897:910	arg1	column					922:927	DEAE-Sepharose fast flow column	897:927	DEAE-Sepharose fast flow column	897:927	MATERIALS AND METHODS TPP was extracted from T. platycarpum using hot water extraction, ethanol precipitation method and its fractionated using DEAE-Sepharose fast flow column.
34390795	9	7	theme	TPP	1222:1224	arg1	effect					1212:1217	The structure effect	1198:1217	The structure effect of TPP on RAW264.7 cells	1198:1242	The structure effect of TPP on RAW264.7 cells was studied by the removal of sulfate (desulfation) and protein (deproteinization) contents from TPP.
34390795	12	8	theme	MAPK	1840:1843	arg1	activation					1826:1835	the activation	1822:1835	the activation of MAPK and NF-κB pathways via TLR4, TLR2, and CR3 receptors	1822:1896	The polysaccharides activate the RAW264.7 cell to produce a significant amount of NO and upregulate the various mRNA expression by the activation of MAPK and NF-κB pathways via TLR4, TLR2, and CR3 receptors.
34390795	10	9	theme	uronic	1516:1521	arg1	acid					1523:1526	uronic acid	1516:1526	uronic acid	1516:1526	RESULTS We obtained three fractions namely TPP-1, TPP-2, and TPP-3 which mainly consisted of carbohydrates (75.55, 52.71, and 48.41%), sulfate (8.42, 15.19, and 27.67%), uronic acid (1.27, 6.56, and 4.39%), and protein (8.15, 24.85, and 9.73%).
34390795	4	10	from	properties	611:620	arg1	Dahlst					674:679	Taraxacum platycarpum Dahlst	652:679	Taraxacum platycarpum Dahlst	652:679	AIM OF THE STUDY The main objective of this work was to study the composition and structural properties of polysaccharides (TPP) from Taraxacum platycarpum Dahlst.
34390795	12	11	theme	mRNA	1803:1806	arg1	expression					1808:1817	the various mRNA expression	1791:1817	the various mRNA expression	1791:1817	The polysaccharides activate the RAW264.7 cell to produce a significant amount of NO and upregulate the various mRNA expression by the activation of MAPK and NF-κB pathways via TLR4, TLR2, and CR3 receptors.
34390795	4	12	theme	polysaccharides	625:639	arg1	composition					584:594	composition	584:594	composition	584:594	AIM OF THE STUDY The main objective of this work was to study the composition and structural properties of polysaccharides (TPP) from Taraxacum platycarpum Dahlst.
34390795	4	12	theme	polysaccharides	625:639	arg1	properties					611:620	structural properties	600:620	structural properties	600:620	AIM OF THE STUDY The main objective of this work was to study the composition and structural properties of polysaccharides (TPP) from Taraxacum platycarpum Dahlst.
34390795	9	13	theme	sulfate	1274:1280	arg1	removal					1263:1269	the removal	1259:1269	the removal of sulfate (desulfation) and protein (deproteinization) contents from TPP	1259:1343	The structure effect of TPP on RAW264.7 cells was studied by the removal of sulfate (desulfation) and protein (deproteinization) contents from TPP.
34390795	7	14	theme	fractions	1012:1020	arg1	composition					934:944	The composition	930:944	The composition	930:944	The composition, molecular weight, and structural characterization of TPP and its fractions were evaluated by various techniques.
34390795	7	14	theme	fractions	1012:1020	arg1	weight					957:962	molecular weight	947:962	molecular weight	947:962	The composition, molecular weight, and structural characterization of TPP and its fractions were evaluated by various techniques.
34390795	7	14	theme	fractions	1012:1020	arg1	characterization					980:995	structural characterization	969:995	structural characterization	969:995	The composition, molecular weight, and structural characterization of TPP and its fractions were evaluated by various techniques.
34390795	1	15	theme	ETHNOPHARMACOLOGICAL	137:156	arg1	RELEVANCE					158:166	ETHNOPHARMACOLOGICAL RELEVANCE	137:166	ETHNOPHARMACOLOGICAL RELEVANCE	137:166	ETHNOPHARMACOLOGICAL RELEVANCE Taraxacum platycarpum Dahlst.
34390795	11	16	theme	molecular	1603:1611	arg1	56.7					1641:1644	56.7	1641:1644	56.7	1641:1644	The average molecular weight of the fractions was 56.7, 108.2, and 132.3 × 103 g/mol, respectively.
34390795	11	16	theme	molecular	1603:1611	arg1	weight					1613:1618	The average molecular weight	1591:1618	The average molecular weight of the fractions	1591:1635	The average molecular weight of the fractions was 56.7, 108.2, and 132.3 × 103 g/mol, respectively.
34390795	12	17	theme	CR3	1884:1886	arg1	receptors					1888:1896	TLR4, TLR2, and CR3 receptors	1868:1896	TLR4, TLR2, and CR3 receptors	1868:1896	The polysaccharides activate the RAW264.7 cell to produce a significant amount of NO and upregulate the various mRNA expression by the activation of MAPK and NF-κB pathways via TLR4, TLR2, and CR3 receptors.
34390795	4	18	from	composition	584:594	arg1	Dahlst					674:679	Taraxacum platycarpum Dahlst	652:679	Taraxacum platycarpum Dahlst	652:679	AIM OF THE STUDY The main objective of this work was to study the composition and structural properties of polysaccharides (TPP) from Taraxacum platycarpum Dahlst.
34390795	15	19	theme	present	2244:2250	arg1	study					2252:2256	The present study	2240:2256	The present study	2240:2256	CONCLUSIONS The present study demonstrated that the polysaccharide isolated from T. platycarpum shows admirable immunostimulatory by the activation of MAPK and NF-κB pathways through TLR4, TLR2, and CR3 receptors.
34390795	11	20	theme	fractions	1627:1635	arg1	56.7					1641:1644	56.7	1641:1644	56.7	1641:1644	The average molecular weight of the fractions was 56.7, 108.2, and 132.3 × 103 g/mol, respectively.
34390795	11	20	theme	fractions	1627:1635	arg1	weight					1613:1618	The average molecular weight	1591:1618	The average molecular weight of the fractions	1591:1635	The average molecular weight of the fractions was 56.7, 108.2, and 132.3 × 103 g/mol, respectively.
34390795	0	21	theme	MAPK	94:97	arg1	pathways					109:116	MAPK and NF-κB pathways	94:116	MAPK and NF-κB pathways in RAW264.7 cells	94:134	Polysaccharide extracted from Taraxacum platycarpum root exerts immunomodulatory activity via MAPK and NF-κB pathways in RAW264.7 cells.
34390795	9	22	theme	protein	1300:1306	arg1	contents					1327:1334	sulfate (desulfation) and protein (deproteinization) contents	1274:1334	contents	1327:1334	The structure effect of TPP on RAW264.7 cells was studied by the removal of sulfate (desulfation) and protein (deproteinization) contents from TPP.
34390795	9	22	theme	protein	1300:1306	arg1	deproteinization					1309:1324	deproteinization	1309:1324	deproteinization	1309:1324	The structure effect of TPP on RAW264.7 cells was studied by the removal of sulfate (desulfation) and protein (deproteinization) contents from TPP.
34390795	12	23	theme	NO	1773:1774	arg1	amount					1763:1768	a significant amount	1749:1768	a significant amount of NO	1749:1774	The polysaccharides activate the RAW264.7 cell to produce a significant amount of NO and upregulate the various mRNA expression by the activation of MAPK and NF-κB pathways via TLR4, TLR2, and CR3 receptors.
34390795	12	23	theme	NO	1773:1774	arg1	NO					1773:1774	NO	1773:1774	NO	1773:1774	The polysaccharides activate the RAW264.7 cell to produce a significant amount of NO and upregulate the various mRNA expression by the activation of MAPK and NF-κB pathways via TLR4, TLR2, and CR3 receptors.
34390795	7	24	theme	structural	969:978	arg1	characterization					980:995	structural characterization	969:995	structural characterization	969:995	The composition, molecular weight, and structural characterization of TPP and its fractions were evaluated by various techniques.
34390795	3	25	dep	platycarpum	389:399	arg1	leaves					401:406	leaves	401:406	leaves	401:406	Moreover, a decoction prepared from T. platycarpum leaves and stems is an effective treatment for cancer, glycosuria, liver disease, pleurodynia, and stomach problems.
34390795	0	26	theme	NF-κB	103:107	arg1	pathways					109:116	MAPK and NF-κB pathways	94:116	MAPK and NF-κB pathways in RAW264.7 cells	94:134	Polysaccharide extracted from Taraxacum platycarpum root exerts immunomodulatory activity via MAPK and NF-κB pathways in RAW264.7 cells.
34390795	9	27	theme	contents	1327:1334	arg1	removal					1263:1269	the removal	1259:1269	the removal of sulfate (desulfation) and protein (deproteinization) contents from TPP	1259:1343	The structure effect of TPP on RAW264.7 cells was studied by the removal of sulfate (desulfation) and protein (deproteinization) contents from TPP.
34390795	7	28	theme	molecular	947:955	arg1	weight					957:962	molecular weight	947:962	molecular weight	947:962	The composition, molecular weight, and structural characterization of TPP and its fractions were evaluated by various techniques.
34390795	3	29	theme	effective	424:432	arg1	decoction					362:370	a decoction	360:370	a decoction prepared from T. platycarpum leaves and stems	360:416	Moreover, a decoction prepared from T. platycarpum leaves and stems is an effective treatment for cancer, glycosuria, liver disease, pleurodynia, and stomach problems.
34390795	3	29	theme	effective	424:432	arg1	treatment					434:442	an effective treatment	421:442	an effective treatment for cancer, glycosuria, liver disease, pleurodynia, and stomach problems	421:515	Moreover, a decoction prepared from T. platycarpum leaves and stems is an effective treatment for cancer, glycosuria, liver disease, pleurodynia, and stomach problems.
34390795	8	30	theme	immunostimulatory	1073:1089	arg1	activity					1091:1098	the immunostimulatory activity	1069:1098	the immunostimulatory activity of polysaccharides	1069:1117	Further, the immunostimulatory activity of polysaccharides was tested on murine macrophage cell line RAW264.7 by various in vitro assays.
34390795	17	31	theme	immunostimulant	2589:2603	arg1	agent					2605:2609	a new immunostimulant agent	2583:2609	a new immunostimulant agent	2583:2609	Our study results could be useful for developing a new immunostimulant agent.
34390795	9	32	from	effect	1212:1217	arg1	RAW264.7 cells					1229:1242	RAW264.7 cells	1229:1242	RAW264.7 cells	1229:1242	The structure effect of TPP on RAW264.7 cells was studied by the removal of sulfate (desulfation) and protein (deproteinization) contents from TPP.
34390795	10	33	theme	protein	1557:1563	arg1	carbohydrates					1439:1451	carbohydrates	1439:1451	carbohydrates (75.55, 52.71, and 48.41%)	1439:1478	RESULTS We obtained three fractions namely TPP-1, TPP-2, and TPP-3 which mainly consisted of carbohydrates (75.55, 52.71, and 48.41%), sulfate (8.42, 15.19, and 27.67%), uronic acid (1.27, 6.56, and 4.39%), and protein (8.15, 24.85, and 9.73%).
34390795	10	33	theme	protein	1557:1563	arg1	%					1512:1512	8.42, 15.19, and 27.67%	1490:1512	8.42, 15.19, and 27.67%	1490:1512	RESULTS We obtained three fractions namely TPP-1, TPP-2, and TPP-3 which mainly consisted of carbohydrates (75.55, 52.71, and 48.41%), sulfate (8.42, 15.19, and 27.67%), uronic acid (1.27, 6.56, and 4.39%), and protein (8.15, 24.85, and 9.73%).
34390795	10	33	theme	protein	1557:1563	arg1	%					1477:1477	75.55, 52.71, and 48.41%	1454:1477	75.55, 52.71, and 48.41%	1454:1477	RESULTS We obtained three fractions namely TPP-1, TPP-2, and TPP-3 which mainly consisted of carbohydrates (75.55, 52.71, and 48.41%), sulfate (8.42, 15.19, and 27.67%), uronic acid (1.27, 6.56, and 4.39%), and protein (8.15, 24.85, and 9.73%).
34390795	10	33	theme	protein	1557:1563	arg1	%					1549:1549	1.27, 6.56, and 4.39%	1529:1549	1.27, 6.56, and 4.39%	1529:1549	RESULTS We obtained three fractions namely TPP-1, TPP-2, and TPP-3 which mainly consisted of carbohydrates (75.55, 52.71, and 48.41%), sulfate (8.42, 15.19, and 27.67%), uronic acid (1.27, 6.56, and 4.39%), and protein (8.15, 24.85, and 9.73%).
34390795	13	34	theme	TPP-2	2041:2045	arg1	content					2030:2036	the protein content	2018:2036	the protein content of TPP-2	2018:2045	The structurally modified deproteinated derivative (DP-TPP-2) more effectively decreases the NO production which means the protein content of TPP-2 mainly contributes to the RAW264.7 cells activation.
34390795	6	35	theme	ethanol	841:847	arg1	method					863:868	ethanol precipitation method	841:868	ethanol precipitation method	841:868	MATERIALS AND METHODS TPP was extracted from T. platycarpum using hot water extraction, ethanol precipitation method and its fractionated using DEAE-Sepharose fast flow column.
34390795	3	36	dep	cancer	448:453	arg1	problems					508:515	problems	508:515	problems	508:515	Moreover, a decoction prepared from T. platycarpum leaves and stems is an effective treatment for cancer, glycosuria, liver disease, pleurodynia, and stomach problems.
34390795	16	37	theme	polysaccharides	2465:2479	arg1	content					2454:2460	The protein content	2442:2460	The protein content of polysaccharides	2442:2479	The protein content of polysaccharides mainly contributes to the RAW264.7 cells activation.
34390795	8	38	theme	macrophage	1140:1149	arg1	line					1156:1159	murine macrophage cell line	1133:1159	murine macrophage cell line RAW264.7	1133:1168	Further, the immunostimulatory activity of polysaccharides was tested on murine macrophage cell line RAW264.7 by various in vitro assays.
34390795	12	39	theme	TLR4	1868:1871	arg1	receptors					1888:1896	TLR4, TLR2, and CR3 receptors	1868:1896	TLR4, TLR2, and CR3 receptors	1868:1896	The polysaccharides activate the RAW264.7 cell to produce a significant amount of NO and upregulate the various mRNA expression by the activation of MAPK and NF-κB pathways via TLR4, TLR2, and CR3 receptors.
34390795	14	40	dep	-Glup	2167:2171	arg1	1 → 2					2148:2152	1 → 2	2148:2152	1 → 2	2148:2152	The structure of DP-TPP-2 primarily consists of 1 → 2)-Galp, 1 → 6)-Glup, 1 → 2) - Rhap, and 1 → 5) - Arap glycosidic linkages.
34390795	14	40	dep	-Glup	2167:2171	arg1	1 → 6					2161:2165	-Galp, 1 → 6)-Glup	2154:2171	1 → 6	2161:2165	The structure of DP-TPP-2 primarily consists of 1 → 2)-Galp, 1 → 6)-Glup, 1 → 2) - Rhap, and 1 → 5) - Arap glycosidic linkages.
34390795	14	40	dep	-Glup	2167:2171	arg1	1 → 2					2174:2178	1 → 2	2174:2178	1 → 2	2174:2178	The structure of DP-TPP-2 primarily consists of 1 → 2)-Galp, 1 → 6)-Glup, 1 → 2) - Rhap, and 1 → 5) - Arap glycosidic linkages.
34390795	2	41	dep	herb	232:235	arg1	dandelion					206:214	dandelion	206:214	dandelion	206:214	(Korean dandelion) is a medicinal herb used in traditional medicine in Korea to treat various disease such as furuncles, mammitis, hepatitis, jaundice.
34390795	14	42	theme	Arap	2202:2205	arg1	linkages					2218:2225	Arap glycosidic linkages	2202:2225	Arap glycosidic linkages	2202:2225	The structure of DP-TPP-2 primarily consists of 1 → 2)-Galp, 1 → 6)-Glup, 1 → 2) - Rhap, and 1 → 5) - Arap glycosidic linkages.
34390795	4	43	from	Dahlst	674:679	arg1	TPP					642:644	TPP	642:644	TPP	642:644	AIM OF THE STUDY The main objective of this work was to study the composition and structural properties of polysaccharides (TPP) from Taraxacum platycarpum Dahlst.
34390795	4	43	from	Dahlst	674:679	arg1	polysaccharides					625:639	polysaccharides	625:639	polysaccharides (TPP) from Taraxacum platycarpum Dahlst	625:679	AIM OF THE STUDY The main objective of this work was to study the composition and structural properties of polysaccharides (TPP) from Taraxacum platycarpum Dahlst.
34390795	4	43	from	Dahlst	674:679	arg1	composition					584:594	composition	584:594	composition	584:594	AIM OF THE STUDY The main objective of this work was to study the composition and structural properties of polysaccharides (TPP) from Taraxacum platycarpum Dahlst.
34390795	4	43	from	Dahlst	674:679	arg1	properties					611:620	structural properties	600:620	structural properties	600:620	AIM OF THE STUDY The main objective of this work was to study the composition and structural properties of polysaccharides (TPP) from Taraxacum platycarpum Dahlst.
34390795	13	44	theme	NO	1992:1993	arg1	production					1995:2004	the NO production	1988:2004	the NO production which means the protein content of TPP-2 mainly contributes to the RAW264.7 cells activation	1988:2097	The structurally modified deproteinated derivative (DP-TPP-2) more effectively decreases the NO production which means the protein content of TPP-2 mainly contributes to the RAW264.7 cells activation.
34390795	12	45	theme	TLR2	1874:1877	arg1	receptors					1888:1896	TLR4, TLR2, and CR3 receptors	1868:1896	TLR4, TLR2, and CR3 receptors	1868:1896	The polysaccharides activate the RAW264.7 cell to produce a significant amount of NO and upregulate the various mRNA expression by the activation of MAPK and NF-κB pathways via TLR4, TLR2, and CR3 receptors.
34390795	17	46	theme	study	2538:2542	arg1	results					2544:2550	Our study results	2534:2550	Our study results	2534:2550	Our study results could be useful for developing a new immunostimulant agent.
34390795	9	47	theme	structure	1202:1210	arg1	effect					1212:1217	The structure effect	1198:1217	The structure effect of TPP on RAW264.7 cells	1198:1242	The structure effect of TPP on RAW264.7 cells was studied by the removal of sulfate (desulfation) and protein (deproteinization) contents from TPP.
34390795	15	48	theme	NF-κB	2388:2392	arg1	pathways					2394:2401	NF-κB pathways	2388:2401	NF-κB pathways	2388:2401	CONCLUSIONS The present study demonstrated that the polysaccharide isolated from T. platycarpum shows admirable immunostimulatory by the activation of MAPK and NF-κB pathways through TLR4, TLR2, and CR3 receptors.
34390795	8	49	theme	in	1181:1182	arg1	assays					1190:1195	various in vitro assays	1173:1195	various in vitro assays	1173:1195	Further, the immunostimulatory activity of polysaccharides was tested on murine macrophage cell line RAW264.7 by various in vitro assays.
34390795	6	50	theme	fast	912:915	arg1	column					922:927	DEAE-Sepharose fast flow column	897:927	DEAE-Sepharose fast flow column	897:927	MATERIALS AND METHODS TPP was extracted from T. platycarpum using hot water extraction, ethanol precipitation method and its fractionated using DEAE-Sepharose fast flow column.
34390795	10	51	dep	obtained	1357:1364	arg1	RESULTS					1346:1352	RESULTS	1346:1352	RESULTS	1346:1352	RESULTS We obtained three fractions namely TPP-1, TPP-2, and TPP-3 which mainly consisted of carbohydrates (75.55, 52.71, and 48.41%), sulfate (8.42, 15.19, and 27.67%), uronic acid (1.27, 6.56, and 4.39%), and protein (8.15, 24.85, and 9.73%).
34390795	12	52	theme	pathways	1855:1862	arg1	activation					1826:1835	the activation	1822:1835	the activation of MAPK and NF-κB pathways via TLR4, TLR2, and CR3 receptors	1822:1896	The polysaccharides activate the RAW264.7 cell to produce a significant amount of NO and upregulate the various mRNA expression by the activation of MAPK and NF-κB pathways via TLR4, TLR2, and CR3 receptors.
34390795	10	53	dep	fractions	1372:1380	arg1	TPP-2					1396:1400	TPP-2	1396:1400	TPP-2	1396:1400	RESULTS We obtained three fractions namely TPP-1, TPP-2, and TPP-3 which mainly consisted of carbohydrates (75.55, 52.71, and 48.41%), sulfate (8.42, 15.19, and 27.67%), uronic acid (1.27, 6.56, and 4.39%), and protein (8.15, 24.85, and 9.73%).
34390795	10	53	dep	fractions	1372:1380	arg1	TPP-3					1407:1411	TPP-3	1407:1411	TPP-3	1407:1411	RESULTS We obtained three fractions namely TPP-1, TPP-2, and TPP-3 which mainly consisted of carbohydrates (75.55, 52.71, and 48.41%), sulfate (8.42, 15.19, and 27.67%), uronic acid (1.27, 6.56, and 4.39%), and protein (8.15, 24.85, and 9.73%).
34390795	10	53	dep	fractions	1372:1380	arg1	TPP-1					1389:1393	TPP-1	1389:1393	TPP-1	1389:1393	RESULTS We obtained three fractions namely TPP-1, TPP-2, and TPP-3 which mainly consisted of carbohydrates (75.55, 52.71, and 48.41%), sulfate (8.42, 15.19, and 27.67%), uronic acid (1.27, 6.56, and 4.39%), and protein (8.15, 24.85, and 9.73%).
34390795	10	53	dep	fractions	1372:1380	arg1	fractions					1372:1380	three fractions	1366:1380	three fractions namely TPP-1, TPP-2, and TPP-3 which mainly consisted of carbohydrates (75.55, 52.71, and 48.41%), sulfate (8.42, 15.19, and 27.67%), uronic acid (1.27, 6.56, and 4.39%), and protein (8.15, 24.85, and 9.73%)	1366:1588	RESULTS We obtained three fractions namely TPP-1, TPP-2, and TPP-3 which mainly consisted of carbohydrates (75.55, 52.71, and 48.41%), sulfate (8.42, 15.19, and 27.67%), uronic acid (1.27, 6.56, and 4.39%), and protein (8.15, 24.85, and 9.73%).
34390795	10	53	dep	fractions	1372:1380	arg1	%					1587:1587	9.73%	1583:1587	9.73%	1583:1587	RESULTS We obtained three fractions namely TPP-1, TPP-2, and TPP-3 which mainly consisted of carbohydrates (75.55, 52.71, and 48.41%), sulfate (8.42, 15.19, and 27.67%), uronic acid (1.27, 6.56, and 4.39%), and protein (8.15, 24.85, and 9.73%).
34390795	13	54	theme	modified	1916:1923	arg1	DP-TPP-2					1951:1958	DP-TPP-2	1951:1958	DP-TPP-2	1951:1958	The structurally modified deproteinated derivative (DP-TPP-2) more effectively decreases the NO production which means the protein content of TPP-2 mainly contributes to the RAW264.7 cells activation.
34390795	13	54	theme	modified	1916:1923	arg1	derivative					1939:1948	The structurally modified deproteinated derivative	1899:1948	The structurally modified deproteinated derivative (DP-TPP-2)	1899:1959	The structurally modified deproteinated derivative (DP-TPP-2) more effectively decreases the NO production which means the protein content of TPP-2 mainly contributes to the RAW264.7 cells activation.
34390795	15	55	dep	CONCLUSIONS	2228:2238	arg1	demonstrated					2258:2269	demonstrated	2258:2269	demonstrated that the polysaccharide isolated from T. platycarpum shows admirable immunostimulatory by the activation of MAPK and NF-κB pathways through TLR4, TLR2, and CR3 receptors	2258:2439	CONCLUSIONS The present study demonstrated that the polysaccharide isolated from T. platycarpum shows admirable immunostimulatory by the activation of MAPK and NF-κB pathways through TLR4, TLR2, and CR3 receptors.
34390795	4	56	theme	structural	600:609	arg1	properties					611:620	structural properties	600:620	structural properties	600:620	AIM OF THE STUDY The main objective of this work was to study the composition and structural properties of polysaccharides (TPP) from Taraxacum platycarpum Dahlst.
34390795	4	57	dep	was	567:569	arg1	AIM					518:520	AIM	518:520	AIM OF THE STUDY	518:533	AIM OF THE STUDY The main objective of this work was to study the composition and structural properties of polysaccharides (TPP) from Taraxacum platycarpum Dahlst.
34390795	15	58	theme	CR3	2427:2429	arg1	receptors					2431:2439	CR3 receptors	2427:2439	CR3 receptors	2427:2439	CONCLUSIONS The present study demonstrated that the polysaccharide isolated from T. platycarpum shows admirable immunostimulatory by the activation of MAPK and NF-κB pathways through TLR4, TLR2, and CR3 receptors.
34390795	12	59	theme	various	1795:1801	arg1	expression					1808:1817	the various mRNA expression	1791:1817	the various mRNA expression	1791:1817	The polysaccharides activate the RAW264.7 cell to produce a significant amount of NO and upregulate the various mRNA expression by the activation of MAPK and NF-κB pathways via TLR4, TLR2, and CR3 receptors.
34390795	16	60	theme	protein	2446:2452	arg1	content					2454:2460	The protein content	2442:2460	The protein content of polysaccharides	2442:2479	The protein content of polysaccharides mainly contributes to the RAW264.7 cells activation.
34390795	5	61	from	activity	725:732	arg1	RAW264.7 cells					737:750	RAW264.7 cells	737:750	RAW264.7 cells	737:750	root and investigate the immunostimulatory activity on RAW264.7 cells.
34390795	2	62	theme	traditional	245:255	arg1	medicine					257:264	traditional medicine	245:264	traditional medicine in Korea	245:273	(Korean dandelion) is a medicinal herb used in traditional medicine in Korea to treat various disease such as furuncles, mammitis, hepatitis, jaundice.
34390795	7	63	theme	various	1040:1046	arg1	techniques					1048:1057	various techniques	1040:1057	various techniques	1040:1057	The composition, molecular weight, and structural characterization of TPP and its fractions were evaluated by various techniques.
34390795	4	64	dep	composition	584:594	arg1	the					580:582	the	580:582	the	580:582	AIM OF THE STUDY The main objective of this work was to study the composition and structural properties of polysaccharides (TPP) from Taraxacum platycarpum Dahlst.
34390795	2	65	theme	various	284:290	arg1	disease					292:298	various disease	284:298	various disease	284:298	(Korean dandelion) is a medicinal herb used in traditional medicine in Korea to treat various disease such as furuncles, mammitis, hepatitis, jaundice.
34390795	0	66	from	pathways	109:116	arg1	RAW264.7 cells					121:134	RAW264.7 cells	121:134	RAW264.7 cells	121:134	Polysaccharide extracted from Taraxacum platycarpum root exerts immunomodulatory activity via MAPK and NF-κB pathways in RAW264.7 cells.
34390795	4	67	theme	Taraxacum	652:660	arg1	Dahlst					674:679	Taraxacum platycarpum Dahlst	652:679	Taraxacum platycarpum Dahlst	652:679	AIM OF THE STUDY The main objective of this work was to study the composition and structural properties of polysaccharides (TPP) from Taraxacum platycarpum Dahlst.
34390795	11	68	theme	average	1595:1601	arg1	56.7					1641:1644	56.7	1641:1644	56.7	1641:1644	The average molecular weight of the fractions was 56.7, 108.2, and 132.3 × 103 g/mol, respectively.
34390795	11	68	theme	average	1595:1601	arg1	weight					1613:1618	The average molecular weight	1591:1618	The average molecular weight of the fractions	1591:1635	The average molecular weight of the fractions was 56.7, 108.2, and 132.3 × 103 g/mol, respectively.
34390795	7	69	theme	TPP	1000:1002	arg1	composition					934:944	The composition	930:944	The composition	930:944	The composition, molecular weight, and structural characterization of TPP and its fractions were evaluated by various techniques.
34390795	7	69	theme	TPP	1000:1002	arg1	weight					957:962	molecular weight	947:962	molecular weight	947:962	The composition, molecular weight, and structural characterization of TPP and its fractions were evaluated by various techniques.
34390795	7	69	theme	TPP	1000:1002	arg1	characterization					980:995	structural characterization	969:995	structural characterization	969:995	The composition, molecular weight, and structural characterization of TPP and its fractions were evaluated by various techniques.
34390795	14	70	theme	-Galp	2154:2158	arg1	-Glup					2167:2171	-Galp, 1 → 6)-Glup	2154:2171	-Glup	2167:2171	The structure of DP-TPP-2 primarily consists of 1 → 2)-Galp, 1 → 6)-Glup, 1 → 2) - Rhap, and 1 → 5) - Arap glycosidic linkages.
34390795	5	71	theme	immunostimulatory	707:723	arg1	activity					725:732	the immunostimulatory activity	703:732	the immunostimulatory activity on RAW264.7 cells	703:750	root and investigate the immunostimulatory activity on RAW264.7 cells.
34390795	13	72	theme	deproteinated	1925:1937	arg1	DP-TPP-2					1951:1958	DP-TPP-2	1951:1958	DP-TPP-2	1951:1958	The structurally modified deproteinated derivative (DP-TPP-2) more effectively decreases the NO production which means the protein content of TPP-2 mainly contributes to the RAW264.7 cells activation.
34390795	13	72	theme	deproteinated	1925:1937	arg1	derivative					1939:1948	The structurally modified deproteinated derivative	1899:1948	The structurally modified deproteinated derivative (DP-TPP-2)	1899:1959	The structurally modified deproteinated derivative (DP-TPP-2) more effectively decreases the NO production which means the protein content of TPP-2 mainly contributes to the RAW264.7 cells activation.
34390795	15	73	theme	MAPK	2379:2382	arg1	activation					2365:2374	the activation	2361:2374	the activation of MAPK and NF-κB pathways through TLR4, TLR2, and CR3 receptors	2361:2439	CONCLUSIONS The present study demonstrated that the polysaccharide isolated from T. platycarpum shows admirable immunostimulatory by the activation of MAPK and NF-κB pathways through TLR4, TLR2, and CR3 receptors.
34390795	8	74	dep	in	1181:1182	arg1	vitro					1184:1188	vitro	1184:1188	vitro	1184:1188	Further, the immunostimulatory activity of polysaccharides was tested on murine macrophage cell line RAW264.7 by various in vitro assays.
34390795	12	75	theme	significant	1751:1761	arg1	amount					1763:1768	a significant amount	1749:1768	a significant amount of NO	1749:1774	The polysaccharides activate the RAW264.7 cell to produce a significant amount of NO and upregulate the various mRNA expression by the activation of MAPK and NF-κB pathways via TLR4, TLR2, and CR3 receptors.
34390795	12	75	theme	significant	1751:1761	arg1	NO					1773:1774	NO	1773:1774	NO	1773:1774	The polysaccharides activate the RAW264.7 cell to produce a significant amount of NO and upregulate the various mRNA expression by the activation of MAPK and NF-κB pathways via TLR4, TLR2, and CR3 receptors.
34390795	15	76	attach	isolated	2295:2302	arg2	polysaccharide					2280:2293	the polysaccharide	2276:2293	the polysaccharide isolated from T. platycarpum	2276:2322	CONCLUSIONS The present study demonstrated that the polysaccharide isolated from T. platycarpum shows admirable immunostimulatory by the activation of MAPK and NF-κB pathways through TLR4, TLR2, and CR3 receptors.
34390795	15	76	attach	isolated	2295:2302	arg1	platycarpum					2312:2322	T. platycarpum	2309:2322	T. platycarpum	2309:2322	CONCLUSIONS The present study demonstrated that the polysaccharide isolated from T. platycarpum shows admirable immunostimulatory by the activation of MAPK and NF-κB pathways through TLR4, TLR2, and CR3 receptors.
34390795	9	77	from	TPP	1341:1343	arg1	removal					1263:1269	the removal	1259:1269	the removal of sulfate (desulfation) and protein (deproteinization) contents from TPP	1259:1343	The structure effect of TPP on RAW264.7 cells was studied by the removal of sulfate (desulfation) and protein (deproteinization) contents from TPP.
34390795	2	78	from	medicine	257:264	arg1	Korea					269:273	Korea	269:273	Korea	269:273	(Korean dandelion) is a medicinal herb used in traditional medicine in Korea to treat various disease such as furuncles, mammitis, hepatitis, jaundice.
34390795	17	79	theme	new	2585:2587	arg1	agent					2605:2609	a new immunostimulant agent	2583:2609	a new immunostimulant agent	2583:2609	Our study results could be useful for developing a new immunostimulant agent.
34390795	4	80	theme	main	539:542	arg1	objective					544:552	The main objective	535:552	The main objective of this work	535:565	AIM OF THE STUDY The main objective of this work was to study the composition and structural properties of polysaccharides (TPP) from Taraxacum platycarpum Dahlst.
34390795	8	81	dep	line	1156:1159	arg1	RAW264.7					1161:1168	RAW264.7	1161:1168	murine macrophage cell line RAW264.7	1133:1168	Further, the immunostimulatory activity of polysaccharides was tested on murine macrophage cell line RAW264.7 by various in vitro assays.
34390795	14	82	theme	DP-TPP-2	2117:2124	arg1	structure					2104:2112	The structure	2100:2112	The structure of DP-TPP-2	2100:2124	The structure of DP-TPP-2 primarily consists of 1 → 2)-Galp, 1 → 6)-Glup, 1 → 2) - Rhap, and 1 → 5) - Arap glycosidic linkages.
34390795	0	83	theme	Taraxacum	30:38	arg1	root					52:55	Taraxacum platycarpum root	30:55	Taraxacum platycarpum root	30:55	Polysaccharide extracted from Taraxacum platycarpum root exerts immunomodulatory activity via MAPK and NF-κB pathways in RAW264.7 cells.
34390795	13	84	theme	RAW264.7 cells	2073:2086	arg1	activation					2088:2097	the RAW264.7 cells activation	2069:2097	the RAW264.7 cells activation	2069:2097	The structurally modified deproteinated derivative (DP-TPP-2) more effectively decreases the NO production which means the protein content of TPP-2 mainly contributes to the RAW264.7 cells activation.
34390795	8	85	theme	polysaccharides	1103:1117	arg1	activity					1091:1098	the immunostimulatory activity	1069:1098	the immunostimulatory activity of polysaccharides	1069:1117	Further, the immunostimulatory activity of polysaccharides was tested on murine macrophage cell line RAW264.7 by various in vitro assays.
34390795	15	86	theme	admirable	2330:2338	arg1	immunostimulatory					2340:2356	admirable immunostimulatory	2330:2356	admirable immunostimulatory by the activation of MAPK and NF-κB pathways through TLR4, TLR2, and CR3 receptors	2330:2439	CONCLUSIONS The present study demonstrated that the polysaccharide isolated from T. platycarpum shows admirable immunostimulatory by the activation of MAPK and NF-κB pathways through TLR4, TLR2, and CR3 receptors.
34390795	1	87	dep	Dahlst	190:195	arg1	RELEVANCE					158:166	ETHNOPHARMACOLOGICAL RELEVANCE	137:166	ETHNOPHARMACOLOGICAL RELEVANCE	137:166	ETHNOPHARMACOLOGICAL RELEVANCE Taraxacum platycarpum Dahlst.
34390795	3	88	theme	liver	468:472	arg1	disease					474:480	liver disease	468:480	liver disease	468:480	Moreover, a decoction prepared from T. platycarpum leaves and stems is an effective treatment for cancer, glycosuria, liver disease, pleurodynia, and stomach problems.
34390795	4	89	theme	platycarpum	662:672	arg1	Dahlst					674:679	Taraxacum platycarpum Dahlst	652:679	Taraxacum platycarpum Dahlst	652:679	AIM OF THE STUDY The main objective of this work was to study the composition and structural properties of polysaccharides (TPP) from Taraxacum platycarpum Dahlst.
34390795	6	90	theme	METHODS	767:773	arg1	TPP					775:777	MATERIALS AND METHODS TPP	753:777	MATERIALS AND METHODS TPP	753:777	MATERIALS AND METHODS TPP was extracted from T. platycarpum using hot water extraction, ethanol precipitation method and its fractionated using DEAE-Sepharose fast flow column.
34390795	0	91	theme	immunomodulatory	64:79	arg1	activity					81:88	immunomodulatory activity	64:88	immunomodulatory activity	64:88	Polysaccharide extracted from Taraxacum platycarpum root exerts immunomodulatory activity via MAPK and NF-κB pathways in RAW264.7 cells.
34390795	8	92	theme	murine	1133:1138	arg1	line					1156:1159	murine macrophage cell line	1133:1159	murine macrophage cell line RAW264.7	1133:1168	Further, the immunostimulatory activity of polysaccharides was tested on murine macrophage cell line RAW264.7 by various in vitro assays.
34390795	6	93	theme	precipitation	849:861	arg1	method					863:868	ethanol precipitation method	841:868	ethanol precipitation method	841:868	MATERIALS AND METHODS TPP was extracted from T. platycarpum using hot water extraction, ethanol precipitation method and its fractionated using DEAE-Sepharose fast flow column.
34390795	4	94	theme	STUDY	529:533	arg1	AIM					518:520	AIM	518:520	AIM OF THE STUDY	518:533	AIM OF THE STUDY The main objective of this work was to study the composition and structural properties of polysaccharides (TPP) from Taraxacum platycarpum Dahlst.
34390795	6	95	theme	MATERIALS	753:761	arg1	TPP					775:777	MATERIALS AND METHODS TPP	753:777	MATERIALS AND METHODS TPP	753:777	MATERIALS AND METHODS TPP was extracted from T. platycarpum using hot water extraction, ethanol precipitation method and its fractionated using DEAE-Sepharose fast flow column.
34390795	8	96	theme	cell	1151:1154	arg1	line					1156:1159	murine macrophage cell line	1133:1159	murine macrophage cell line RAW264.7	1133:1168	Further, the immunostimulatory activity of polysaccharides was tested on murine macrophage cell line RAW264.7 by various in vitro assays.
34390795	0	97	dep	Taraxacum	30:38	arg1	platycarpum					40:50	platycarpum	40:50	platycarpum	40:50	Polysaccharide extracted from Taraxacum platycarpum root exerts immunomodulatory activity via MAPK and NF-κB pathways in RAW264.7 cells.
34390795	2	98	theme	medicinal	222:230	arg1	herb					232:235	a medicinal herb	220:235	a medicinal herb used in traditional medicine in Korea to treat various disease such as furuncles, mammitis, hepatitis, jaundice	220:347	(Korean dandelion) is a medicinal herb used in traditional medicine in Korea to treat various disease such as furuncles, mammitis, hepatitis, jaundice.
34390795	13	99	theme	protein	2022:2028	arg1	content					2030:2036	the protein content	2018:2036	the protein content of TPP-2	2018:2045	The structurally modified deproteinated derivative (DP-TPP-2) more effectively decreases the NO production which means the protein content of TPP-2 mainly contributes to the RAW264.7 cells activation.
34390795	6	100	theme	water	823:827	arg1	extraction					829:838	hot water extraction	819:838	hot water extraction	819:838	MATERIALS AND METHODS TPP was extracted from T. platycarpum using hot water extraction, ethanol precipitation method and its fractionated using DEAE-Sepharose fast flow column.
34390795	16	101	theme	RAW264.7 cells	2507:2520	arg1	activation					2522:2531	the RAW264.7 cells activation	2503:2531	the RAW264.7 cells activation	2503:2531	The protein content of polysaccharides mainly contributes to the RAW264.7 cells activation.
34390795	14	102	theme	glycosidic	2207:2216	arg1	linkages					2218:2225	Arap glycosidic linkages	2202:2225	Arap glycosidic linkages	2202:2225	The structure of DP-TPP-2 primarily consists of 1 → 2)-Galp, 1 → 6)-Glup, 1 → 2) - Rhap, and 1 → 5) - Arap glycosidic linkages.
34390795	15	103	theme	pathways	2394:2401	arg1	activation					2365:2374	the activation	2361:2374	the activation of MAPK and NF-κB pathways through TLR4, TLR2, and CR3 receptors	2361:2439	CONCLUSIONS The present study demonstrated that the polysaccharide isolated from T. platycarpum shows admirable immunostimulatory by the activation of MAPK and NF-κB pathways through TLR4, TLR2, and CR3 receptors.
34044924	10	0	theme	dependent	1071:1079	arg1	responses					1061:1069	different responses	1051:1069	different responses dependent on their molecular weight, related to the immune regulatory pathways	1051:1148	The immunological activity of these glucans seems to have different responses dependent on their molecular weight, related to the immune regulatory pathways.
34044924	10	1	theme	different	1051:1059	arg1	responses					1061:1069	different responses	1051:1069	different responses dependent on their molecular weight, related to the immune regulatory pathways	1051:1148	The immunological activity of these glucans seems to have different responses dependent on their molecular weight, related to the immune regulatory pathways.
34044924	0	2	theme	dried	75:79	arg1	Longan					81:86	fresh and dried Longan	65:86	fresh and dried Longan (Dimocarpus longan Lour.)	65:112	In vitro immunomodulatory activity of water-soluble glucans from fresh and dried Longan (Dimocarpus longan Lour.)
34044924	0	2	theme	dried	75:79	arg1	Lour					107:110	Lour	107:110	Lour	107:110	In vitro immunomodulatory activity of water-soluble glucans from fresh and dried Longan (Dimocarpus longan Lour.)
34044924	8	3	theme	NO	872:873	arg1	assays					915:920	in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays	849:920	in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays	849:920	These glucans from fresh and dried longan pulp have immunomodulatory activity, shown by in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays.
34044924	2	4	theme	seasonal	153:160	arg1	fruit					171:175	a seasonal tropical fruit	151:175	a seasonal tropical fruit used by Chinese medicine in both fresh and dried pulp forms	151:235	is a seasonal tropical fruit used by Chinese medicine in both fresh and dried pulp forms.
34044924	10	5	theme	molecular	1090:1098	arg1	weight					1100:1105	their molecular weight	1084:1105	their molecular weight	1084:1105	The immunological activity of these glucans seems to have different responses dependent on their molecular weight, related to the immune regulatory pathways.
34044924	8	6	theme	TNF-α	876:880	arg1	assays					915:920	in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays	849:920	in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays	849:920	These glucans from fresh and dried longan pulp have immunomodulatory activity, shown by in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays.
34044924	10	7	theme	regulatory	1130:1139	arg1	pathways					1141:1148	the immune regulatory pathways	1119:1148	the immune regulatory pathways	1119:1148	The immunological activity of these glucans seems to have different responses dependent on their molecular weight, related to the immune regulatory pathways.
34044924	2	8	from	medicine	193:200	arg1	forms					231:235	both fresh and dried pulp forms	205:235	both fresh and dried pulp forms	205:235	is a seasonal tropical fruit used by Chinese medicine in both fresh and dried pulp forms.
34044924	8	9	dep	in	849:850	arg1	vitro					852:856	vitro	852:856	vitro	852:856	These glucans from fresh and dried longan pulp have immunomodulatory activity, shown by in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays.
34044924	5	10	theme	dried	451:455	arg1	pulp					464:467	fresh and dried longan pulp	441:467	fresh and dried longan pulp	441:467	To fulfil this aim, hot water-soluble polysaccharides of fresh and dried longan pulp were fractionated according to their molecular weight by ultrafiltration (10, 50, 100 kDa cut off).
34044924	8	11	theme	IL-6	887:890	arg1	macrophages					892:902	IL-6 macrophages	887:902	IL-6 macrophages production	887:913	These glucans from fresh and dried longan pulp have immunomodulatory activity, shown by in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays.
34044924	5	12	theme	longan	457:462	arg1	pulp					464:467	fresh and dried longan pulp	441:467	fresh and dried longan pulp	441:467	To fulfil this aim, hot water-soluble polysaccharides of fresh and dried longan pulp were fractionated according to their molecular weight by ultrafiltration (10, 50, 100 kDa cut off).
34044924	4	13	theme	immune	339:344	arg1	activity					346:353	immune activity	339:353	immune activity	339:353	However, their composition and immune activity have not yet been disclosed.
34044924	5	14	theme	pulp	464:467	arg1	polysaccharides					422:436	hot water-soluble polysaccharides	404:436	hot water-soluble polysaccharides of fresh and dried longan pulp	404:467	To fulfil this aim, hot water-soluble polysaccharides of fresh and dried longan pulp were fractionated according to their molecular weight by ultrafiltration (10, 50, 100 kDa cut off).
34044924	3	15	theme	biological	287:296	arg1	activity					298:305	biological activity	287:305	biological activity	287:305	Their polysaccharides have been reported to have biological activity.
34044924	8	16	theme	production	904:913	arg1	assays					915:920	in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays	849:920	in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays	849:920	These glucans from fresh and dried longan pulp have immunomodulatory activity, shown by in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays.
34044924	8	17	theme	in	849:850	arg1	phagocytosis					858:869	in vitro phagocytosis	849:869	in vitro phagocytosis	849:869	These glucans from fresh and dried longan pulp have immunomodulatory activity, shown by in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays.
34044924	7	18	theme	drying	698:703	arg1	process					705:711	The drying process	694:711	The drying process	694:711	The drying process promotes the solubility of the polysaccharides.
34044924	0	19	theme	In	0:1	arg1	activity					26:33	In vitro immunomodulatory activity	0:33	In vitro immunomodulatory activity of water-soluble glucans from fresh and dried Longan (Dimocarpus longan Lour.)	0:112	In vitro immunomodulatory activity of water-soluble glucans from fresh and dried Longan (Dimocarpus longan Lour.)
34044924	8	20	contain	have	808:811	arg2	activity					830:837	immunomodulatory activity	813:837	immunomodulatory activity	813:837	These glucans from fresh and dried longan pulp have immunomodulatory activity, shown by in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays.
34044924	8	20	contain	have	808:811	arg1	glucans					767:773	These glucans	761:773	These glucans from fresh and dried longan pulp	761:806	These glucans from fresh and dried longan pulp have immunomodulatory activity, shown by in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays.
34044924	8	21	theme	macrophages	892:902	arg1	production					904:913	IL-6 macrophages production	887:913	IL-6 macrophages production	887:913	These glucans from fresh and dried longan pulp have immunomodulatory activity, shown by in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays.
34044924	10	22	contain	have	1046:1049	arg2	responses					1061:1069	different responses	1051:1069	different responses dependent on their molecular weight, related to the immune regulatory pathways	1051:1148	The immunological activity of these glucans seems to have different responses dependent on their molecular weight, related to the immune regulatory pathways.
34044924	10	22	contain	have	1046:1049	arg1	activity					1011:1018	The immunological activity	993:1018	The immunological activity of these glucans	993:1035	The immunological activity of these glucans seems to have different responses dependent on their molecular weight, related to the immune regulatory pathways.
34044924	7	23	theme	polysaccharides	744:758	arg1	solubility					726:735	the solubility	722:735	the solubility of the polysaccharides	722:758	The drying process promotes the solubility of the polysaccharides.
34044924	10	24	theme	immune	1123:1128	arg1	pathways					1141:1148	the immune regulatory pathways	1119:1148	the immune regulatory pathways	1119:1148	The immunological activity of these glucans seems to have different responses dependent on their molecular weight, related to the immune regulatory pathways.
34044924	8	25	theme	fresh	780:784	arg1	pulp					803:806	fresh and dried longan pulp	780:806	fresh and dried longan pulp	780:806	These glucans from fresh and dried longan pulp have immunomodulatory activity, shown by in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays.
34044924	8	26	theme	phagocytosis	858:869	arg1	assays					915:920	in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays	849:920	in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays	849:920	These glucans from fresh and dried longan pulp have immunomodulatory activity, shown by in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays.
34044924	0	27	theme	immunomodulatory	9:24	arg1	activity					26:33	In vitro immunomodulatory activity	0:33	In vitro immunomodulatory activity of water-soluble glucans from fresh and dried Longan (Dimocarpus longan Lour.)	0:112	In vitro immunomodulatory activity of water-soluble glucans from fresh and dried Longan (Dimocarpus longan Lour.)
34044924	0	28	from	Longan	81:86	arg1	activity					26:33	In vitro immunomodulatory activity	0:33	In vitro immunomodulatory activity of water-soluble glucans from fresh and dried Longan (Dimocarpus longan Lour.)	0:112	In vitro immunomodulatory activity of water-soluble glucans from fresh and dried Longan (Dimocarpus longan Lour.)
34044924	5	29	theme	fresh	441:445	arg1	pulp					464:467	fresh and dried longan pulp	441:467	fresh and dried longan pulp	441:467	To fulfil this aim, hot water-soluble polysaccharides of fresh and dried longan pulp were fractionated according to their molecular weight by ultrafiltration (10, 50, 100 kDa cut off).
34044924	0	30	theme	water-soluble	38:50	arg1	glucans					52:58	water-soluble glucans	38:58	water-soluble glucans	38:58	In vitro immunomodulatory activity of water-soluble glucans from fresh and dried Longan (Dimocarpus longan Lour.)
34044924	5	31	dep	ultrafiltration	526:540	arg1	cut					559:561	10, 50, 100 kDa cut	543:561	10, 50, 100 kDa cut	543:561	To fulfil this aim, hot water-soluble polysaccharides of fresh and dried longan pulp were fractionated according to their molecular weight by ultrafiltration (10, 50, 100 kDa cut off).
34044924	6	32	link	1,6-linked	609:618	arg1	glucans					620:626	1,6-linked glucans	609:626	1,6-linked glucans branched at O-3 (4-8%), O-2 (1%), O-2,4 (0.1%), and O-3,4 (0.1%)	609:691	The main polysaccharides recovered were 1,6-linked glucans branched at O-3 (4-8%), O-2 (1%), O-2,4 (0.1%), and O-3,4 (0.1%).
34044924	6	32	link	1,6-linked	609:618	arg1	polysaccharides					578:592	The main polysaccharides	569:592	The main polysaccharides recovered	569:602	The main polysaccharides recovered were 1,6-linked glucans branched at O-3 (4-8%), O-2 (1%), O-2,4 (0.1%), and O-3,4 (0.1%).
34044924	8	33	theme	longan	796:801	arg1	pulp					803:806	fresh and dried longan pulp	780:806	fresh and dried longan pulp	780:806	These glucans from fresh and dried longan pulp have immunomodulatory activity, shown by in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays.
34044924	8	34	from	pulp	803:806	arg1	glucans					767:773	These glucans	761:773	These glucans from fresh and dried longan pulp	761:806	These glucans from fresh and dried longan pulp have immunomodulatory activity, shown by in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays.
34044924	6	35	theme	1,6-linked	609:618	arg1	glucans					620:626	1,6-linked glucans	609:626	1,6-linked glucans branched at O-3 (4-8%), O-2 (1%), O-2,4 (0.1%), and O-3,4 (0.1%)	609:691	The main polysaccharides recovered were 1,6-linked glucans branched at O-3 (4-8%), O-2 (1%), O-2,4 (0.1%), and O-3,4 (0.1%).
34044924	6	35	theme	1,6-linked	609:618	arg1	polysaccharides					578:592	The main polysaccharides	569:592	The main polysaccharides recovered	569:602	The main polysaccharides recovered were 1,6-linked glucans branched at O-3 (4-8%), O-2 (1%), O-2,4 (0.1%), and O-3,4 (0.1%).
34044924	10	36	theme	immunological	997:1009	arg1	activity					1011:1018	The immunological activity	993:1018	The immunological activity of these glucans	993:1035	The immunological activity of these glucans seems to have different responses dependent on their molecular weight, related to the immune regulatory pathways.
34044924	9	37	theme	inflammatory	955:966	arg1	response					968:975	the inflammatory response	951:975	the inflammatory response induced by LPS	951:990	They showed also to inhibit the inflammatory response induced by LPS.
34044924	5	38	theme	molecular	506:514	arg1	weight					516:521	their molecular weight	500:521	their molecular weight	500:521	To fulfil this aim, hot water-soluble polysaccharides of fresh and dried longan pulp were fractionated according to their molecular weight by ultrafiltration (10, 50, 100 kDa cut off).
34044924	0	39	theme	glucans	52:58	arg1	activity					26:33	In vitro immunomodulatory activity	0:33	In vitro immunomodulatory activity of water-soluble glucans from fresh and dried Longan (Dimocarpus longan Lour.)	0:112	In vitro immunomodulatory activity of water-soluble glucans from fresh and dried Longan (Dimocarpus longan Lour.)
34044924	2	40	theme	Chinese	185:191	arg1	medicine					193:200	Chinese medicine	185:200	Chinese medicine in both fresh and dried pulp forms	185:235	is a seasonal tropical fruit used by Chinese medicine in both fresh and dried pulp forms.
34044924	8	41	theme	dried	790:794	arg1	pulp					803:806	fresh and dried longan pulp	780:806	fresh and dried longan pulp	780:806	These glucans from fresh and dried longan pulp have immunomodulatory activity, shown by in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays.
34044924	5	42	dep	cut	559:561	arg1	off					563:565	off	563:565	off	563:565	To fulfil this aim, hot water-soluble polysaccharides of fresh and dried longan pulp were fractionated according to their molecular weight by ultrafiltration (10, 50, 100 kDa cut off).
34044924	2	43	theme	fresh	210:214	arg1	forms					231:235	both fresh and dried pulp forms	205:235	both fresh and dried pulp forms	205:235	is a seasonal tropical fruit used by Chinese medicine in both fresh and dried pulp forms.
34044924	2	44	theme	pulp	226:229	arg1	forms					231:235	both fresh and dried pulp forms	205:235	both fresh and dried pulp forms	205:235	is a seasonal tropical fruit used by Chinese medicine in both fresh and dried pulp forms.
34044924	10	45	theme	related	1108:1114	arg1	weight					1100:1105	their molecular weight	1084:1105	their molecular weight	1084:1105	The immunological activity of these glucans seems to have different responses dependent on their molecular weight, related to the immune regulatory pathways.
34044924	10	46	theme	glucans	1029:1035	arg1	activity					1011:1018	The immunological activity	993:1018	The immunological activity of these glucans	993:1035	The immunological activity of these glucans seems to have different responses dependent on their molecular weight, related to the immune regulatory pathways.
34044924	2	47	theme	dried	220:224	arg1	forms					231:235	both fresh and dried pulp forms	205:235	both fresh and dried pulp forms	205:235	is a seasonal tropical fruit used by Chinese medicine in both fresh and dried pulp forms.
34044924	5	48	theme	hot	404:406	arg1	polysaccharides					422:436	hot water-soluble polysaccharides	404:436	hot water-soluble polysaccharides of fresh and dried longan pulp	404:467	To fulfil this aim, hot water-soluble polysaccharides of fresh and dried longan pulp were fractionated according to their molecular weight by ultrafiltration (10, 50, 100 kDa cut off).
34044924	2	49	theme	tropical	162:169	arg1	fruit					171:175	a seasonal tropical fruit	151:175	a seasonal tropical fruit used by Chinese medicine in both fresh and dried pulp forms	151:235	is a seasonal tropical fruit used by Chinese medicine in both fresh and dried pulp forms.
34044924	3	50	contain	have	282:285	arg1	polysaccharides					244:258	Their polysaccharides	238:258	Their polysaccharides	238:258	Their polysaccharides have been reported to have biological activity.
34044924	3	50	contain	have	282:285	arg2	activity					298:305	biological activity	287:305	biological activity	287:305	Their polysaccharides have been reported to have biological activity.
34044924	5	51	theme	water-soluble	408:420	arg1	polysaccharides					422:436	hot water-soluble polysaccharides	404:436	hot water-soluble polysaccharides of fresh and dried longan pulp	404:467	To fulfil this aim, hot water-soluble polysaccharides of fresh and dried longan pulp were fractionated according to their molecular weight by ultrafiltration (10, 50, 100 kDa cut off).
34044924	0	52	theme	fresh	65:69	arg1	Longan					81:86	fresh and dried Longan	65:86	fresh and dried Longan (Dimocarpus longan Lour.)	65:112	In vitro immunomodulatory activity of water-soluble glucans from fresh and dried Longan (Dimocarpus longan Lour.)
34044924	0	52	theme	fresh	65:69	arg1	Lour					107:110	Lour	107:110	Lour	107:110	In vitro immunomodulatory activity of water-soluble glucans from fresh and dried Longan (Dimocarpus longan Lour.)
34044924	0	53	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro immunomodulatory activity of water-soluble glucans from fresh and dried Longan (Dimocarpus longan Lour.)
34044924	8	54	theme	immunomodulatory	813:828	arg1	activity					830:837	immunomodulatory activity	813:837	immunomodulatory activity	813:837	These glucans from fresh and dried longan pulp have immunomodulatory activity, shown by in vitro phagocytosis, NO, TNF-α, and IL-6 macrophages production assays.
34044924	6	55	theme	main	573:576	arg1	glucans					620:626	1,6-linked glucans	609:626	1,6-linked glucans branched at O-3 (4-8%), O-2 (1%), O-2,4 (0.1%), and O-3,4 (0.1%)	609:691	The main polysaccharides recovered were 1,6-linked glucans branched at O-3 (4-8%), O-2 (1%), O-2,4 (0.1%), and O-3,4 (0.1%).
34044924	6	55	theme	main	573:576	arg1	polysaccharides					578:592	The main polysaccharides	569:592	The main polysaccharides recovered	569:602	The main polysaccharides recovered were 1,6-linked glucans branched at O-3 (4-8%), O-2 (1%), O-2,4 (0.1%), and O-3,4 (0.1%).
34464667	1	0	theme	vinyl	317:321	arg1	alcohol					323:329	vinyl alcohol	317:329	vinyl alcohol	317:329	This work was intended to prepare biodegradable and edible films from polysaccharide extracted from Hammada scoparia leaves (named PSP) and reinforced by poly (vinyl alcohol) (PVA).
34464667	1	0	theme	vinyl	317:321	arg1	poly					311:314	poly	311:314	poly (vinyl alcohol) (PVA)	311:336	This work was intended to prepare biodegradable and edible films from polysaccharide extracted from Hammada scoparia leaves (named PSP) and reinforced by poly (vinyl alcohol) (PVA).
34464667	2	1	theme	optical	562:568	arg1	TGA					594:596	TGA	594:596	TGA	594:596	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	2	1	theme	optical	562:568	arg1	properties					582:591	structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties	489:591	structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA)	489:597	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	3	2	theme	healing	726:732	arg1	capability					734:743	wound healing capability	720:743	wound healing capability	720:743	The antioxidant activities of different films were determined in vitro and evaluated in vivo through the examination of wound healing capability.
34464667	0	3	theme	films	85:89	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of a novel hamada scoparia polysaccharide composite films and evaluation of their effect on cutaneous wound healing in rat.
34464667	0	3	theme	films	85:89	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of a novel hamada scoparia polysaccharide composite films and evaluation of their effect on cutaneous wound healing in rat.
34464667	2	4	theme	pure	424:427	arg1	PVA					429:431	pure PVA	424:431	pure PVA (100 % PVA)	424:443	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	2	4	theme	pure	424:427	arg1	films					344:348	Four films	339:348	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA)	339:443	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	2	4	theme	pure	424:427	arg1	PVA					440:442	100 % PVA	434:442	100 % PVA	434:442	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	2	5	theme	physical	509:516	arg1	TGA					594:596	TGA	594:596	TGA	594:596	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	2	5	theme	physical	509:516	arg1	properties					582:591	structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties	489:591	structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA)	489:597	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	2	6	dep	physical	509:516	arg1	solubility					530:539	solubility	530:539	solubility	530:539	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	2	6	dep	physical	509:516	arg1	index					554:558	swelling index	545:558	swelling index	545:558	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	2	6	dep	physical	509:516	arg1	Thickness					519:527	Thickness	519:527	Thickness	519:527	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	0	7	theme	evaluation	95:104	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of a novel hamada scoparia polysaccharide composite films and evaluation of their effect on cutaneous wound healing in rat.
34464667	0	7	theme	evaluation	95:104	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of a novel hamada scoparia polysaccharide composite films and evaluation of their effect on cutaneous wound healing in rat.
34464667	4	8	theme	CYTOL	1086:1090	arg1	CENTELLA®					1092:1100	CYTOL CENTELLA®	1086:1100	CYTOL CENTELLA®	1086:1100	Data revealed that the film P1 displayed the highest antioxidant activity in vitro and accelerated significantly the wound healing, after sixteen days of treatment, attested by higher wound appearance scores and a higher content of collagen (765.924 ± 4.44 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL CENTELLA® and pure PVA-treated groups.
34464667	5	9	theme	PSP/PVA	1172:1178	arg1	films					1186:1190	PSP/PVA based films	1172:1190	PSP/PVA based films	1172:1190	Overall, these results demonstrated that PSP/PVA based films exhibited a higher wound healing potential confirmed with the high antioxidant activities in vitro.
34464667	0	10	theme	effect	115:120	arg1	films					85:89	a novel hamada scoparia polysaccharide composite films	36:89	a novel hamada scoparia polysaccharide composite films	36:89	Preparation and characterization of a novel hamada scoparia polysaccharide composite films and evaluation of their effect on cutaneous wound healing in rat.
34464667	0	10	theme	effect	115:120	arg1	evaluation					95:104	evaluation	95:104	evaluation of their effect on cutaneous wound healing in rat	95:154	Preparation and characterization of a novel hamada scoparia polysaccharide composite films and evaluation of their effect on cutaneous wound healing in rat.
34464667	2	11	theme	PSP/PVA	375:381	arg1	ratios					365:370	different ratios	355:370	different ratios of PSP/PVA	355:381	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	4	12	theme	treatment	900:908	arg1	days					892:895	sixteen days	884:895	sixteen days of treatment	884:908	Data revealed that the film P1 displayed the highest antioxidant activity in vitro and accelerated significantly the wound healing, after sixteen days of treatment, attested by higher wound appearance scores and a higher content of collagen (765.924 ± 4.44 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL CENTELLA® and pure PVA-treated groups.
34464667	4	13	theme	higher	960:965	arg1	content					967:973	a higher content	958:973	a higher content of collagen (765.924 ± 4.44 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL CENTELLA® and pure PVA-treated groups	958:1128	Data revealed that the film P1 displayed the highest antioxidant activity in vitro and accelerated significantly the wound healing, after sixteen days of treatment, attested by higher wound appearance scores and a higher content of collagen (765.924 ± 4.44 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL CENTELLA® and pure PVA-treated groups.
34464667	2	14	theme	different	355:363	arg1	ratios					365:370	different ratios	355:370	different ratios of PSP/PVA	355:381	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	4	15	theme	highest	791:797	arg1	activity					811:818	the highest antioxidant activity	787:818	the highest antioxidant activity	787:818	Data revealed that the film P1 displayed the highest antioxidant activity in vitro and accelerated significantly the wound healing, after sixteen days of treatment, attested by higher wound appearance scores and a higher content of collagen (765.924 ± 4.44 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL CENTELLA® and pure PVA-treated groups.
34464667	5	16	theme	high	1254:1257	arg1	activities					1271:1280	the high antioxidant activities	1250:1280	the high antioxidant activities	1250:1280	Overall, these results demonstrated that PSP/PVA based films exhibited a higher wound healing potential confirmed with the high antioxidant activities in vitro.
34464667	3	17	theme	capability	734:743	arg1	examination					705:715	the examination	701:715	the examination of wound healing capability	701:743	The antioxidant activities of different films were determined in vitro and evaluated in vivo through the examination of wound healing capability.
34464667	4	18	theme	antioxidant	799:809	arg1	activity					811:818	the highest antioxidant activity	787:818	the highest antioxidant activity	787:818	Data revealed that the film P1 displayed the highest antioxidant activity in vitro and accelerated significantly the wound healing, after sixteen days of treatment, attested by higher wound appearance scores and a higher content of collagen (765.924 ± 4.44 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL CENTELLA® and pure PVA-treated groups.
34464667	2	19	dep	structural	489:498	arg1	FT-IR					501:505	FT-IR	501:505	FT-IR	501:505	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	2	20	theme	structural	489:498	arg1	TGA					594:596	TGA	594:596	TGA	594:596	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	2	20	theme	structural	489:498	arg1	properties					582:591	structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties	489:591	structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA)	489:597	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	5	21	theme	antioxidant	1259:1269	arg1	activities					1271:1280	the high antioxidant activities	1250:1280	the high antioxidant activities	1250:1280	Overall, these results demonstrated that PSP/PVA based films exhibited a higher wound healing potential confirmed with the high antioxidant activities in vitro.
34464667	0	22	from	evaluation	95:104	arg1	healing					141:147	cutaneous wound healing	125:147	cutaneous wound healing in rat	125:154	Preparation and characterization of a novel hamada scoparia polysaccharide composite films and evaluation of their effect on cutaneous wound healing in rat.
34464667	4	23	theme	wound	863:867	arg1	healing					869:875	the wound healing	859:875	the wound healing	859:875	Data revealed that the film P1 displayed the highest antioxidant activity in vitro and accelerated significantly the wound healing, after sixteen days of treatment, attested by higher wound appearance scores and a higher content of collagen (765.924 ± 4.44 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL CENTELLA® and pure PVA-treated groups.
34464667	0	24	theme	cutaneous	125:133	arg1	healing					141:147	cutaneous wound healing	125:147	cutaneous wound healing in rat	125:154	Preparation and characterization of a novel hamada scoparia polysaccharide composite films and evaluation of their effect on cutaneous wound healing in rat.
34464667	4	25	theme	appearance	936:945	arg1	scores					947:952	higher wound appearance scores	923:952	higher wound appearance scores	923:952	Data revealed that the film P1 displayed the highest antioxidant activity in vitro and accelerated significantly the wound healing, after sixteen days of treatment, attested by higher wound appearance scores and a higher content of collagen (765.924 ± 4.44 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL CENTELLA® and pure PVA-treated groups.
34464667	5	26	theme	higher	1204:1209	arg1	healing					1217:1223	a higher wound healing potential	1202:1233	a higher wound healing potential confirmed with the high antioxidant activities in vitro	1202:1289	Overall, these results demonstrated that PSP/PVA based films exhibited a higher wound healing potential confirmed with the high antioxidant activities in vitro.
34464667	1	27	dep	polysaccharide	227:240	arg1	reinforced					297:306	reinforced	297:306	reinforced by poly (vinyl alcohol) (PVA)	297:336	This work was intended to prepare biodegradable and edible films from polysaccharide extracted from Hammada scoparia leaves (named PSP) and reinforced by poly (vinyl alcohol) (PVA).
34464667	1	27	dep	polysaccharide	227:240	arg1	extracted					242:250	extracted	242:250	extracted from Hammada scoparia leaves (named PSP)	242:291	This work was intended to prepare biodegradable and edible films from polysaccharide extracted from Hammada scoparia leaves (named PSP) and reinforced by poly (vinyl alcohol) (PVA).
34464667	1	28	theme	biodegradable	191:203	arg1	films					216:220	biodegradable and edible films	191:220	biodegradable and edible films from polysaccharide extracted from Hammada scoparia leaves (named PSP) and reinforced by poly (vinyl alcohol) (PVA)	191:336	This work was intended to prepare biodegradable and edible films from polysaccharide extracted from Hammada scoparia leaves (named PSP) and reinforced by poly (vinyl alcohol) (PVA).
34464667	0	29	theme	novel	38:42	arg1	films					85:89	a novel hamada scoparia polysaccharide composite films	36:89	a novel hamada scoparia polysaccharide composite films	36:89	Preparation and characterization of a novel hamada scoparia polysaccharide composite films and evaluation of their effect on cutaneous wound healing in rat.
34464667	5	30	theme	based	1180:1184	arg1	films					1186:1190	PSP/PVA based films	1172:1190	PSP/PVA based films	1172:1190	Overall, these results demonstrated that PSP/PVA based films exhibited a higher wound healing potential confirmed with the high antioxidant activities in vitro.
34464667	0	31	from	films	85:89	arg1	healing					141:147	cutaneous wound healing	125:147	cutaneous wound healing in rat	125:154	Preparation and characterization of a novel hamada scoparia polysaccharide composite films and evaluation of their effect on cutaneous wound healing in rat.
34464667	4	32	theme	film	769:772	arg1	P1					774:775	the film P1	765:775	the film P1	765:775	Data revealed that the film P1 displayed the highest antioxidant activity in vitro and accelerated significantly the wound healing, after sixteen days of treatment, attested by higher wound appearance scores and a higher content of collagen (765.924 ± 4.44 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL CENTELLA® and pure PVA-treated groups.
34464667	0	33	theme	wound	135:139	arg1	healing					141:147	cutaneous wound healing	125:147	cutaneous wound healing in rat	125:154	Preparation and characterization of a novel hamada scoparia polysaccharide composite films and evaluation of their effect on cutaneous wound healing in rat.
34464667	5	34	theme	potential	1225:1233	arg1	healing					1217:1223	a higher wound healing potential	1202:1233	a higher wound healing potential confirmed with the high antioxidant activities in vitro	1202:1289	Overall, these results demonstrated that PSP/PVA based films exhibited a higher wound healing potential confirmed with the high antioxidant activities in vitro.
34464667	1	35	theme	edible	209:214	arg1	films					216:220	biodegradable and edible films	191:220	biodegradable and edible films from polysaccharide extracted from Hammada scoparia leaves (named PSP) and reinforced by poly (vinyl alcohol) (PVA)	191:336	This work was intended to prepare biodegradable and edible films from polysaccharide extracted from Hammada scoparia leaves (named PSP) and reinforced by poly (vinyl alcohol) (PVA).
34464667	0	36	theme	scoparia	51:58	arg1	films					85:89	a novel hamada scoparia polysaccharide composite films	36:89	a novel hamada scoparia polysaccharide composite films	36:89	Preparation and characterization of a novel hamada scoparia polysaccharide composite films and evaluation of their effect on cutaneous wound healing in rat.
34464667	1	37	from	polysaccharide	227:240	arg1	films					216:220	biodegradable and edible films	191:220	biodegradable and edible films from polysaccharide extracted from Hammada scoparia leaves (named PSP) and reinforced by poly (vinyl alcohol) (PVA)	191:336	This work was intended to prepare biodegradable and edible films from polysaccharide extracted from Hammada scoparia leaves (named PSP) and reinforced by poly (vinyl alcohol) (PVA).
34464667	0	38	theme	hamada	44:49	arg1	films					85:89	a novel hamada scoparia polysaccharide composite films	36:89	a novel hamada scoparia polysaccharide composite films	36:89	Preparation and characterization of a novel hamada scoparia polysaccharide composite films and evaluation of their effect on cutaneous wound healing in rat.
34464667	4	39	dep	collagen	978:985	arg1	765.924 ± 4.44 mg/g					988:1006	765.924 ± 4.44 mg/g	988:1006	765.924 ± 4.44 mg/g of tissue	988:1016	Data revealed that the film P1 displayed the highest antioxidant activity in vitro and accelerated significantly the wound healing, after sixteen days of treatment, attested by higher wound appearance scores and a higher content of collagen (765.924 ± 4.44 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL CENTELLA® and pure PVA-treated groups.
34464667	2	40	theme	%	438:438	arg1	PVA					429:431	pure PVA	424:431	pure PVA (100 % PVA)	424:443	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	2	40	theme	%	438:438	arg1	PVA					440:442	100 % PVA	434:442	100 % PVA	434:442	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	0	41	from	effect	115:120	arg1	healing					141:147	cutaneous wound healing	125:147	cutaneous wound healing in rat	125:154	Preparation and characterization of a novel hamada scoparia polysaccharide composite films and evaluation of their effect on cutaneous wound healing in rat.
34464667	4	42	theme	histological	1032:1043	arg1	examination					1045:1055	histological examination	1032:1055	histological examination	1032:1055	Data revealed that the film P1 displayed the highest antioxidant activity in vitro and accelerated significantly the wound healing, after sixteen days of treatment, attested by higher wound appearance scores and a higher content of collagen (765.924 ± 4.44 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL CENTELLA® and pure PVA-treated groups.
34464667	3	43	theme	antioxidant	604:614	arg1	activities					616:625	The antioxidant activities	600:625	The antioxidant activities of different films	600:644	The antioxidant activities of different films were determined in vitro and evaluated in vivo through the examination of wound healing capability.
34464667	2	44	dep	films	344:348	arg1	P1					384:385	P1 (70:30)	384:393	P1 (70:30)	384:393	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	2	44	dep	films	344:348	arg1	PVA					440:442	100 % PVA	434:442	100 % PVA	434:442	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	2	44	dep	films	344:348	arg1	FP3					408:410	FP3 (30:70)	408:418	FP3 (30:70)	408:418	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	2	44	dep	films	344:348	arg1	PVA					429:431	pure PVA	424:431	pure PVA (100 % PVA)	424:443	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	2	44	dep	films	344:348	arg1	P2					396:397	P2 (50:50)	396:405	P2 (50:50)	396:405	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	2	44	dep	films	344:348	arg1	films					344:348	Four films	339:348	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA)	339:443	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	2	45	theme	swelling	545:552	arg1	index					554:558	swelling index	545:558	swelling index	545:558	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	4	46	theme	pure	1106:1109	arg1	groups					1123:1128	pure PVA-treated groups	1106:1128	pure PVA-treated groups	1106:1128	Data revealed that the film P1 displayed the highest antioxidant activity in vitro and accelerated significantly the wound healing, after sixteen days of treatment, attested by higher wound appearance scores and a higher content of collagen (765.924 ± 4.44 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL CENTELLA® and pure PVA-treated groups.
34464667	5	47	theme	wound	1211:1215	arg1	healing					1217:1223	a higher wound healing potential	1202:1233	a higher wound healing potential confirmed with the high antioxidant activities in vitro	1202:1289	Overall, these results demonstrated that PSP/PVA based films exhibited a higher wound healing potential confirmed with the high antioxidant activities in vitro.
34464667	0	48	from	healing	141:147	arg1	films					85:89	a novel hamada scoparia polysaccharide composite films	36:89	a novel hamada scoparia polysaccharide composite films	36:89	Preparation and characterization of a novel hamada scoparia polysaccharide composite films and evaluation of their effect on cutaneous wound healing in rat.
34464667	0	48	from	healing	141:147	arg1	rat					152:154	rat	152:154	rat	152:154	Preparation and characterization of a novel hamada scoparia polysaccharide composite films and evaluation of their effect on cutaneous wound healing in rat.
34464667	0	48	from	healing	141:147	arg1	evaluation					95:104	evaluation	95:104	evaluation of their effect on cutaneous wound healing in rat	95:154	Preparation and characterization of a novel hamada scoparia polysaccharide composite films and evaluation of their effect on cutaneous wound healing in rat.
34464667	4	49	theme	PVA-treated	1111:1121	arg1	groups					1123:1128	pure PVA-treated groups	1106:1128	pure PVA-treated groups	1106:1128	Data revealed that the film P1 displayed the highest antioxidant activity in vitro and accelerated significantly the wound healing, after sixteen days of treatment, attested by higher wound appearance scores and a higher content of collagen (765.924 ± 4.44 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL CENTELLA® and pure PVA-treated groups.
34464667	2	50	theme	properties	582:591	arg1	terms					480:484	terms	480:484	terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA)	480:597	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	4	51	theme	wound	930:934	arg1	scores					947:952	higher wound appearance scores	923:952	higher wound appearance scores	923:952	Data revealed that the film P1 displayed the highest antioxidant activity in vitro and accelerated significantly the wound healing, after sixteen days of treatment, attested by higher wound appearance scores and a higher content of collagen (765.924 ± 4.44 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL CENTELLA® and pure PVA-treated groups.
34464667	4	52	theme	collagen	978:985	arg1	content					967:973	a higher content	958:973	a higher content of collagen (765.924 ± 4.44 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL CENTELLA® and pure PVA-treated groups	958:1128	Data revealed that the film P1 displayed the highest antioxidant activity in vitro and accelerated significantly the wound healing, after sixteen days of treatment, attested by higher wound appearance scores and a higher content of collagen (765.924 ± 4.44 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL CENTELLA® and pure PVA-treated groups.
34464667	4	52	theme	collagen	978:985	arg1	scores					947:952	higher wound appearance scores	923:952	higher wound appearance scores	923:952	Data revealed that the film P1 displayed the highest antioxidant activity in vitro and accelerated significantly the wound healing, after sixteen days of treatment, attested by higher wound appearance scores and a higher content of collagen (765.924 ± 4.44 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL CENTELLA® and pure PVA-treated groups.
34464667	0	53	theme	composite	75:83	arg1	films					85:89	a novel hamada scoparia polysaccharide composite films	36:89	a novel hamada scoparia polysaccharide composite films	36:89	Preparation and characterization of a novel hamada scoparia polysaccharide composite films and evaluation of their effect on cutaneous wound healing in rat.
34464667	1	54	dep	Hammada	257:263	arg1	scoparia					265:272	Hammada scoparia	257:272	Hammada scoparia leaves (named PSP)	257:291	This work was intended to prepare biodegradable and edible films from polysaccharide extracted from Hammada scoparia leaves (named PSP) and reinforced by poly (vinyl alcohol) (PVA).
34464667	1	54	dep	Hammada	257:263	arg1	leaves					274:279	leaves	274:279	leaves	274:279	This work was intended to prepare biodegradable and edible films from polysaccharide extracted from Hammada scoparia leaves (named PSP) and reinforced by poly (vinyl alcohol) (PVA).
34464667	2	55	theme	thermal	574:580	arg1	TGA					594:596	TGA	594:596	TGA	594:596	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	2	55	theme	thermal	574:580	arg1	properties					582:591	structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties	489:591	structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA)	489:597	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	4	56	theme	higher	923:928	arg1	scores					947:952	higher wound appearance scores	923:952	higher wound appearance scores	923:952	Data revealed that the film P1 displayed the highest antioxidant activity in vitro and accelerated significantly the wound healing, after sixteen days of treatment, attested by higher wound appearance scores and a higher content of collagen (765.924 ± 4.44 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL CENTELLA® and pure PVA-treated groups.
34464667	3	57	theme	different	630:638	arg1	films					640:644	different films	630:644	different films	630:644	The antioxidant activities of different films were determined in vitro and evaluated in vivo through the examination of wound healing capability.
34464667	0	58	theme	polysaccharide	60:73	arg1	films					85:89	a novel hamada scoparia polysaccharide composite films	36:89	a novel hamada scoparia polysaccharide composite films	36:89	Preparation and characterization of a novel hamada scoparia polysaccharide composite films and evaluation of their effect on cutaneous wound healing in rat.
34464667	2	59	with	films	344:348	arg1	ratios					365:370	different ratios	355:370	different ratios of PSP/PVA	355:381	Four films with different ratios of PSP/PVA: P1 (70:30), P2 (50:50), FP3 (30:70) and pure PVA (100 % PVA) were prepared and characterized in terms of structural (FT-IR), physical (Thickness, solubility and swelling index), optical and thermal properties (TGA).
34464667	4	60	theme	tissue	1011:1016	arg1	765.924 ± 4.44 mg/g					988:1006	765.924 ± 4.44 mg/g	988:1006	765.924 ± 4.44 mg/g of tissue	988:1016	Data revealed that the film P1 displayed the highest antioxidant activity in vitro and accelerated significantly the wound healing, after sixteen days of treatment, attested by higher wound appearance scores and a higher content of collagen (765.924 ± 4.44 mg/g of tissue) confirmed by histological examination, when compared with control, CYTOL CENTELLA® and pure PVA-treated groups.
34464667	3	61	theme	wound	720:724	arg1	healing					726:732	wound healing	720:732	wound healing capability	720:743	The antioxidant activities of different films were determined in vitro and evaluated in vivo through the examination of wound healing capability.
34464667	3	62	theme	films	640:644	arg1	activities					616:625	The antioxidant activities	600:625	The antioxidant activities of different films	600:644	The antioxidant activities of different films were determined in vitro and evaluated in vivo through the examination of wound healing capability.
34758705	1	0	dep	environment	297:307	arg1	the					293:295	the	293:295	the	293:295	The removal of unsymmetrical dimethylhydrazine (UDMH) has long been a concern because of its harmful effect on the environment and humans.
34758705	4	1	theme	temperature	734:744	arg1	effects					711:717	The effects	707:717	The effects of initial pH, temperature, adsorbent dosage, initial concentration, contact time, and recyclability on the UDMH adsorption behaviour of GO/CMC	707:861	The effects of initial pH, temperature, adsorbent dosage, initial concentration, contact time, and recyclability on the UDMH adsorption behaviour of GO/CMC were studied.
34758705	2	2	theme	novel	351:355	arg1	oxide/O-carboxymethyl					366:386	a novel graphene oxide/O-carboxymethyl	349:386	a novel graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite adsorbent	349:424	This study aimed to prepare a novel graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite adsorbent using the solution-blending method for the removal of UDMH from wastewater.
34758705	0	3	theme	composite	85:93	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite and its unsymmetrical dimethylhydrazine (UDMH) adsorption performance from wastewater.
34758705	0	3	theme	composite	85:93	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite and its unsymmetrical dimethylhydrazine (UDMH) adsorption performance from wastewater.
34758705	7	4	theme	hydrogen	1204:1211	arg1	bonding					1213:1219	hydrogen bonding	1204:1219	hydrogen bonding	1204:1219	The adsorption mechanisms were mainly electrostatic attraction, hydrogen bonding, and surface complexation.
34758705	5	5	theme	kinetics	945:952	arg1	model					954:958	the pseudo-second-order kinetics model	921:958	the pseudo-second-order kinetics model	921:958	The adsorption kinetics was consistent with the pseudo-second-order kinetics model, and the adsorption process was mainly controlled by chemisorption.
34758705	0	6	theme	unsymmetrical	103:115	arg1	performance					153:163	its unsymmetrical dimethylhydrazine (UDMH) adsorption performance	99:163	its unsymmetrical dimethylhydrazine (UDMH) adsorption performance	99:163	Preparation and characterization of graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite and its unsymmetrical dimethylhydrazine (UDMH) adsorption performance from wastewater.
34758705	9	7	theme	designed	1373:1380	arg1	composites					1389:1398	The designed GO/CMC composites	1369:1398	The designed GO/CMC composites	1369:1398	The designed GO/CMC composites exhibited a relatively satisfactory recyclability and removal efficiency after five adsorption-desorption cycles.
34758705	7	8	theme	electrostatic	1178:1190	arg1	attraction					1192:1201	electrostatic attraction	1178:1201	electrostatic attraction	1178:1201	The adsorption mechanisms were mainly electrostatic attraction, hydrogen bonding, and surface complexation.
34758705	7	8	theme	electrostatic	1178:1190	arg1	mechanisms					1155:1164	The adsorption mechanisms	1140:1164	The adsorption mechanisms	1140:1164	The adsorption mechanisms were mainly electrostatic attraction, hydrogen bonding, and surface complexation.
34758705	3	9	theme	infrared	576:583	arg1	microscopy					613:622	Fourier-transform infrared, Raman, scanning electronic microscopy	558:622	microscopy	613:622	The prepared GO/CMC was systematically characterized by Fourier-transform infrared, Raman, scanning electronic microscopy, transmission electron microscopy, thermogravimetric, and zeta potential analyses.
34758705	0	10	from	wastewater	170:179	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite and its unsymmetrical dimethylhydrazine (UDMH) adsorption performance from wastewater.
34758705	0	10	from	wastewater	170:179	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite and its unsymmetrical dimethylhydrazine (UDMH) adsorption performance from wastewater.
34758705	4	11	theme	concentration	773:785	arg1	effects					711:717	The effects	707:717	The effects of initial pH, temperature, adsorbent dosage, initial concentration, contact time, and recyclability on the UDMH adsorption behaviour of GO/CMC	707:861	The effects of initial pH, temperature, adsorbent dosage, initial concentration, contact time, and recyclability on the UDMH adsorption behaviour of GO/CMC were studied.
34758705	9	12	theme	GO/CMC	1382:1387	arg1	composites					1389:1398	The designed GO/CMC composites	1369:1398	The designed GO/CMC composites	1369:1398	The designed GO/CMC composites exhibited a relatively satisfactory recyclability and removal efficiency after five adsorption-desorption cycles.
34758705	4	13	theme	time	796:799	arg1	effects					711:717	The effects	707:717	The effects of initial pH, temperature, adsorbent dosage, initial concentration, contact time, and recyclability on the UDMH adsorption behaviour of GO/CMC	707:861	The effects of initial pH, temperature, adsorbent dosage, initial concentration, contact time, and recyclability on the UDMH adsorption behaviour of GO/CMC were studied.
34758705	1	14	theme	harmful	275:281	arg1	effect					283:288	its harmful effect	271:288	its harmful effect on the environment and humans	271:318	The removal of unsymmetrical dimethylhydrazine (UDMH) has long been a concern because of its harmful effect on the environment and humans.
34758705	4	15	theme	recyclability	806:818	arg1	effects					711:717	The effects	707:717	The effects of initial pH, temperature, adsorbent dosage, initial concentration, contact time, and recyclability on the UDMH adsorption behaviour of GO/CMC	707:861	The effects of initial pH, temperature, adsorbent dosage, initial concentration, contact time, and recyclability on the UDMH adsorption behaviour of GO/CMC were studied.
34758705	4	16	from	effects	711:717	arg1	behaviour					843:851	the UDMH adsorption behaviour	823:851	the UDMH adsorption behaviour of GO/CMC	823:861	The effects of initial pH, temperature, adsorbent dosage, initial concentration, contact time, and recyclability on the UDMH adsorption behaviour of GO/CMC were studied.
34758705	3	17	dep	microscopy	613:622	arg1	Raman					586:590	Fourier-transform infrared, Raman, scanning electronic microscopy	558:622	Raman	586:590	The prepared GO/CMC was systematically characterized by Fourier-transform infrared, Raman, scanning electronic microscopy, transmission electron microscopy, thermogravimetric, and zeta potential analyses.
34758705	0	18	theme	dimethylhydrazine	117:133	arg1	performance					153:163	its unsymmetrical dimethylhydrazine (UDMH) adsorption performance	99:163	its unsymmetrical dimethylhydrazine (UDMH) adsorption performance	99:163	Preparation and characterization of graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite and its unsymmetrical dimethylhydrazine (UDMH) adsorption performance from wastewater.
34758705	3	19	theme	Fourier-transform	558:574	arg1	microscopy					613:622	Fourier-transform infrared, Raman, scanning electronic microscopy	558:622	microscopy	613:622	The prepared GO/CMC was systematically characterized by Fourier-transform infrared, Raman, scanning electronic microscopy, transmission electron microscopy, thermogravimetric, and zeta potential analyses.
34758705	6	20	dep	adsorption	1119:1128	arg1	isotherm					1130:1137	isotherm	1130:1137	the Langmuir adsorption isotherm	1106:1137	Adsorption isotherms indicated that the adsorption of UDMH by GO/CMC followed the Langmuir adsorption isotherm.
34758705	1	21	from	effect	283:288	arg1	humans					313:318	humans	313:318	humans	313:318	The removal of unsymmetrical dimethylhydrazine (UDMH) has long been a concern because of its harmful effect on the environment and humans.
34758705	1	21	from	effect	283:288	arg1	environment					297:307	environment	297:307	environment	297:307	The removal of unsymmetrical dimethylhydrazine (UDMH) has long been a concern because of its harmful effect on the environment and humans.
34758705	3	22	theme	scanning	593:600	arg1	microscopy					613:622	Fourier-transform infrared, Raman, scanning electronic microscopy	558:622	microscopy	613:622	The prepared GO/CMC was systematically characterized by Fourier-transform infrared, Raman, scanning electronic microscopy, transmission electron microscopy, thermogravimetric, and zeta potential analyses.
34758705	1	23	theme	unsymmetrical	197:209	arg1	UDMH					230:233	UDMH	230:233	UDMH	230:233	The removal of unsymmetrical dimethylhydrazine (UDMH) has long been a concern because of its harmful effect on the environment and humans.
34758705	1	23	theme	unsymmetrical	197:209	arg1	dimethylhydrazine					211:227	unsymmetrical dimethylhydrazine	197:227	unsymmetrical dimethylhydrazine (UDMH)	197:234	The removal of unsymmetrical dimethylhydrazine (UDMH) has long been a concern because of its harmful effect on the environment and humans.
34758705	8	24	theme	GO/CMC	1261:1266	arg1	wastewater					1357:1366	a renewable and eco-friendly adsorbent for the removal of UDMH wastewater	1294:1366	a renewable and eco-friendly adsorbent for the removal of UDMH wastewater	1294:1366	Furthermore, GO/CMC composites can be used as a renewable and eco-friendly adsorbent for the removal of UDMH wastewater.
34758705	8	24	theme	GO/CMC	1261:1266	arg1	composites					1268:1277	GO/CMC composites	1261:1277	GO/CMC composites	1261:1277	Furthermore, GO/CMC composites can be used as a renewable and eco-friendly adsorbent for the removal of UDMH wastewater.
34758705	6	25	theme	Adsorption	1028:1037	arg1	isotherms					1039:1047	Adsorption isotherms	1028:1047	Adsorption isotherms	1028:1047	Adsorption isotherms indicated that the adsorption of UDMH by GO/CMC followed the Langmuir adsorption isotherm.
34758705	3	26	theme	electronic	602:611	arg1	microscopy					613:622	Fourier-transform infrared, Raman, scanning electronic microscopy	558:622	microscopy	613:622	The prepared GO/CMC was systematically characterized by Fourier-transform infrared, Raman, scanning electronic microscopy, transmission electron microscopy, thermogravimetric, and zeta potential analyses.
34758705	0	27	theme	chitosan	67:74	arg1	composite					85:93	graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite	36:93	graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite	36:93	Preparation and characterization of graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite and its unsymmetrical dimethylhydrazine (UDMH) adsorption performance from wastewater.
34758705	1	28	theme	dimethylhydrazine	211:227	arg1	removal					186:192	The removal	182:192	The removal of unsymmetrical dimethylhydrazine (UDMH)	182:234	The removal of unsymmetrical dimethylhydrazine (UDMH) has long been a concern because of its harmful effect on the environment and humans.
34758705	1	28	theme	dimethylhydrazine	211:227	arg1	concern					252:258	a concern	250:258	a concern	250:258	The removal of unsymmetrical dimethylhydrazine (UDMH) has long been a concern because of its harmful effect on the environment and humans.
34758705	4	29	theme	initial	765:771	arg1	concentration					773:785	initial concentration	765:785	initial concentration	765:785	The effects of initial pH, temperature, adsorbent dosage, initial concentration, contact time, and recyclability on the UDMH adsorption behaviour of GO/CMC were studied.
34758705	5	30	theme	adsorption	969:978	arg1	process					980:986	the adsorption process	965:986	the adsorption process	965:986	The adsorption kinetics was consistent with the pseudo-second-order kinetics model, and the adsorption process was mainly controlled by chemisorption.
34758705	4	31	theme	contact	788:794	arg1	time					796:799	contact time	788:799	contact time	788:799	The effects of initial pH, temperature, adsorbent dosage, initial concentration, contact time, and recyclability on the UDMH adsorption behaviour of GO/CMC were studied.
34758705	6	32	theme	Langmuir	1110:1117	arg1	adsorption					1119:1128	the Langmuir adsorption isotherm	1106:1137	the Langmuir adsorption isotherm	1106:1137	Adsorption isotherms indicated that the adsorption of UDMH by GO/CMC followed the Langmuir adsorption isotherm.
34758705	2	33	theme	solution-blending	436:452	arg1	method					454:459	the solution-blending method	432:459	the solution-blending method for the removal of UDMH from wastewater	432:499	This study aimed to prepare a novel graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite adsorbent using the solution-blending method for the removal of UDMH from wastewater.
34758705	9	34	theme	recyclability	1436:1448	arg1	efficiency					1462:1471	a relatively satisfactory recyclability and removal efficiency	1410:1471	a relatively satisfactory recyclability and removal efficiency	1410:1471	The designed GO/CMC composites exhibited a relatively satisfactory recyclability and removal efficiency after five adsorption-desorption cycles.
34758705	0	35	theme	UDMH	136:139	arg1	performance					153:163	its unsymmetrical dimethylhydrazine (UDMH) adsorption performance	99:163	its unsymmetrical dimethylhydrazine (UDMH) adsorption performance	99:163	Preparation and characterization of graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite and its unsymmetrical dimethylhydrazine (UDMH) adsorption performance from wastewater.
34758705	3	36	theme	potential	687:695	arg1	analyses					697:704	zeta potential analyses	682:704	zeta potential analyses	682:704	The prepared GO/CMC was systematically characterized by Fourier-transform infrared, Raman, scanning electronic microscopy, transmission electron microscopy, thermogravimetric, and zeta potential analyses.
34758705	7	37	theme	surface	1226:1232	arg1	complexation					1234:1245	surface complexation	1226:1245	surface complexation	1226:1245	The adsorption mechanisms were mainly electrostatic attraction, hydrogen bonding, and surface complexation.
34758705	0	38	theme	oxide/O-carboxymethyl	45:65	arg1	composite					85:93	graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite	36:93	graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite	36:93	Preparation and characterization of graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite and its unsymmetrical dimethylhydrazine (UDMH) adsorption performance from wastewater.
34758705	0	39	theme	performance	153:163	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite and its unsymmetrical dimethylhydrazine (UDMH) adsorption performance from wastewater.
34758705	0	39	theme	performance	153:163	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite and its unsymmetrical dimethylhydrazine (UDMH) adsorption performance from wastewater.
34758705	2	40	from	wastewater	490:499	arg1	removal					469:475	the removal	465:475	the removal of UDMH from wastewater	465:499	This study aimed to prepare a novel graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite adsorbent using the solution-blending method for the removal of UDMH from wastewater.
34758705	3	41	theme	transmission	625:636	arg1	microscopy					647:656	transmission electron microscopy	625:656	transmission electron microscopy	625:656	The prepared GO/CMC was systematically characterized by Fourier-transform infrared, Raman, scanning electronic microscopy, transmission electron microscopy, thermogravimetric, and zeta potential analyses.
34758705	8	42	theme	adsorbent	1323:1331	arg1	wastewater					1357:1366	a renewable and eco-friendly adsorbent for the removal of UDMH wastewater	1294:1366	a renewable and eco-friendly adsorbent for the removal of UDMH wastewater	1294:1366	Furthermore, GO/CMC composites can be used as a renewable and eco-friendly adsorbent for the removal of UDMH wastewater.
34758705	8	42	theme	adsorbent	1323:1331	arg1	composites					1268:1277	GO/CMC composites	1261:1277	GO/CMC composites	1261:1277	Furthermore, GO/CMC composites can be used as a renewable and eco-friendly adsorbent for the removal of UDMH wastewater.
34758705	2	43	theme	adsorbent	416:424	arg1	oxide/O-carboxymethyl					366:386	a novel graphene oxide/O-carboxymethyl	349:386	a novel graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite adsorbent	349:424	This study aimed to prepare a novel graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite adsorbent using the solution-blending method for the removal of UDMH from wastewater.
34758705	2	44	theme	UDMH	480:483	arg1	removal					469:475	the removal	465:475	the removal of UDMH from wastewater	465:499	This study aimed to prepare a novel graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite adsorbent using the solution-blending method for the removal of UDMH from wastewater.
34758705	9	45	theme	removal	1454:1460	arg1	efficiency					1462:1471	a relatively satisfactory recyclability and removal efficiency	1410:1471	a relatively satisfactory recyclability and removal efficiency	1410:1471	The designed GO/CMC composites exhibited a relatively satisfactory recyclability and removal efficiency after five adsorption-desorption cycles.
34758705	0	46	theme	graphene	36:43	arg1	composite					85:93	graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite	36:93	graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite	36:93	Preparation and characterization of graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite and its unsymmetrical dimethylhydrazine (UDMH) adsorption performance from wastewater.
34758705	8	47	theme	UDMH	1352:1355	arg1	removal					1341:1347	the removal	1337:1347	the removal of UDMH	1337:1355	Furthermore, GO/CMC composites can be used as a renewable and eco-friendly adsorbent for the removal of UDMH wastewater.
34758705	3	48	theme	electron	638:645	arg1	microscopy					647:656	transmission electron microscopy	625:656	transmission electron microscopy	625:656	The prepared GO/CMC was systematically characterized by Fourier-transform infrared, Raman, scanning electronic microscopy, transmission electron microscopy, thermogravimetric, and zeta potential analyses.
34758705	5	49	theme	adsorption	881:890	arg1	consistent					905:914	consistent	905:914	consistent	905:914	The adsorption kinetics was consistent with the pseudo-second-order kinetics model, and the adsorption process was mainly controlled by chemisorption.
34758705	5	49	theme	adsorption	881:890	arg1	kinetics					892:899	The adsorption kinetics	877:899	The adsorption kinetics	877:899	The adsorption kinetics was consistent with the pseudo-second-order kinetics model, and the adsorption process was mainly controlled by chemisorption.
34758705	8	50	theme	renewable	1296:1304	arg1	wastewater					1357:1366	a renewable and eco-friendly adsorbent for the removal of UDMH wastewater	1294:1366	a renewable and eco-friendly adsorbent for the removal of UDMH wastewater	1294:1366	Furthermore, GO/CMC composites can be used as a renewable and eco-friendly adsorbent for the removal of UDMH wastewater.
34758705	8	50	theme	renewable	1296:1304	arg1	composites					1268:1277	GO/CMC composites	1261:1277	GO/CMC composites	1261:1277	Furthermore, GO/CMC composites can be used as a renewable and eco-friendly adsorbent for the removal of UDMH wastewater.
34758705	5	51	with	consistent	905:914	arg1	model					954:958	the pseudo-second-order kinetics model	921:958	the pseudo-second-order kinetics model	921:958	The adsorption kinetics was consistent with the pseudo-second-order kinetics model, and the adsorption process was mainly controlled by chemisorption.
34758705	3	52	theme	zeta	682:685	arg1	analyses					697:704	zeta potential analyses	682:704	zeta potential analyses	682:704	The prepared GO/CMC was systematically characterized by Fourier-transform infrared, Raman, scanning electronic microscopy, transmission electron microscopy, thermogravimetric, and zeta potential analyses.
34758705	4	53	theme	adsorbent	747:755	arg1	dosage					757:762	adsorbent dosage	747:762	adsorbent dosage	747:762	The effects of initial pH, temperature, adsorbent dosage, initial concentration, contact time, and recyclability on the UDMH adsorption behaviour of GO/CMC were studied.
34758705	0	54	theme	adsorption	142:151	arg1	performance					153:163	its unsymmetrical dimethylhydrazine (UDMH) adsorption performance	99:163	its unsymmetrical dimethylhydrazine (UDMH) adsorption performance	99:163	Preparation and characterization of graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite and its unsymmetrical dimethylhydrazine (UDMH) adsorption performance from wastewater.
34758705	7	55	theme	adsorption	1144:1153	arg1	attraction					1192:1201	electrostatic attraction	1178:1201	electrostatic attraction	1178:1201	The adsorption mechanisms were mainly electrostatic attraction, hydrogen bonding, and surface complexation.
34758705	7	55	theme	adsorption	1144:1153	arg1	mechanisms					1155:1164	The adsorption mechanisms	1140:1164	The adsorption mechanisms	1140:1164	The adsorption mechanisms were mainly electrostatic attraction, hydrogen bonding, and surface complexation.
34758705	4	56	theme	GO/CMC	856:861	arg1	behaviour					843:851	the UDMH adsorption behaviour	823:851	the UDMH adsorption behaviour of GO/CMC	823:861	The effects of initial pH, temperature, adsorbent dosage, initial concentration, contact time, and recyclability on the UDMH adsorption behaviour of GO/CMC were studied.
34758705	3	57	theme	prepared	506:513	arg1	GO/CMC					515:520	The prepared GO/CMC	502:520	The prepared GO/CMC	502:520	The prepared GO/CMC was systematically characterized by Fourier-transform infrared, Raman, scanning electronic microscopy, transmission electron microscopy, thermogravimetric, and zeta potential analyses.
34758705	8	58	used	used	1286:1289	arg2	wastewater					1357:1366	a renewable and eco-friendly adsorbent for the removal of UDMH wastewater	1294:1366	a renewable and eco-friendly adsorbent for the removal of UDMH wastewater	1294:1366	Furthermore, GO/CMC composites can be used as a renewable and eco-friendly adsorbent for the removal of UDMH wastewater.
34758705	8	58	used	used	1286:1289	arg2	composites					1268:1277	GO/CMC composites	1261:1277	GO/CMC composites	1261:1277	Furthermore, GO/CMC composites can be used as a renewable and eco-friendly adsorbent for the removal of UDMH wastewater.
34758705	4	59	theme	initial	722:728	arg1	pH					730:731	initial pH	722:731	initial pH	722:731	The effects of initial pH, temperature, adsorbent dosage, initial concentration, contact time, and recyclability on the UDMH adsorption behaviour of GO/CMC were studied.
34758705	9	60	theme	adsorption-desorption	1484:1504	arg1	cycles					1506:1511	five adsorption-desorption cycles	1479:1511	five adsorption-desorption cycles	1479:1511	The designed GO/CMC composites exhibited a relatively satisfactory recyclability and removal efficiency after five adsorption-desorption cycles.
34758705	9	61	theme	satisfactory	1423:1434	arg1	efficiency					1462:1471	a relatively satisfactory recyclability and removal efficiency	1410:1471	a relatively satisfactory recyclability and removal efficiency	1410:1471	The designed GO/CMC composites exhibited a relatively satisfactory recyclability and removal efficiency after five adsorption-desorption cycles.
34758705	4	62	theme	dosage	757:762	arg1	effects					711:717	The effects	707:717	The effects of initial pH, temperature, adsorbent dosage, initial concentration, contact time, and recyclability on the UDMH adsorption behaviour of GO/CMC	707:861	The effects of initial pH, temperature, adsorbent dosage, initial concentration, contact time, and recyclability on the UDMH adsorption behaviour of GO/CMC were studied.
34758705	6	63	theme	UDMH	1082:1085	arg1	adsorption					1068:1077	the adsorption	1064:1077	the adsorption of UDMH by GO/CMC	1064:1095	Adsorption isotherms indicated that the adsorption of UDMH by GO/CMC followed the Langmuir adsorption isotherm.
34758705	5	64	theme	pseudo-second-order	925:943	arg1	model					954:958	the pseudo-second-order kinetics model	921:958	the pseudo-second-order kinetics model	921:958	The adsorption kinetics was consistent with the pseudo-second-order kinetics model, and the adsorption process was mainly controlled by chemisorption.
34758705	4	65	theme	adsorption	832:841	arg1	behaviour					843:851	the UDMH adsorption behaviour	823:851	the UDMH adsorption behaviour of GO/CMC	823:861	The effects of initial pH, temperature, adsorbent dosage, initial concentration, contact time, and recyclability on the UDMH adsorption behaviour of GO/CMC were studied.
34758705	4	66	theme	pH	730:731	arg1	effects					711:717	The effects	707:717	The effects of initial pH, temperature, adsorbent dosage, initial concentration, contact time, and recyclability on the UDMH adsorption behaviour of GO/CMC	707:861	The effects of initial pH, temperature, adsorbent dosage, initial concentration, contact time, and recyclability on the UDMH adsorption behaviour of GO/CMC were studied.
34758705	0	67	theme	GO/CMC	77:82	arg1	composite					85:93	graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite	36:93	graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite	36:93	Preparation and characterization of graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite and its unsymmetrical dimethylhydrazine (UDMH) adsorption performance from wastewater.
34758705	4	68	theme	UDMH	827:830	arg1	behaviour					843:851	the UDMH adsorption behaviour	823:851	the UDMH adsorption behaviour of GO/CMC	823:861	The effects of initial pH, temperature, adsorbent dosage, initial concentration, contact time, and recyclability on the UDMH adsorption behaviour of GO/CMC were studied.
34758705	2	69	theme	graphene	357:364	arg1	oxide/O-carboxymethyl					366:386	a novel graphene oxide/O-carboxymethyl	349:386	a novel graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite adsorbent	349:424	This study aimed to prepare a novel graphene oxide/O-carboxymethyl chitosan (GO/CMC) composite adsorbent using the solution-blending method for the removal of UDMH from wastewater.
34861277	0	0	theme	ions	76:79	arg1	removal					58:64	adsorptive removal	47:64	adsorptive removal of Ni(II) ions from aqueous medium	47:99	Modified alginate-chitosan-TiO2 composites for adsorptive removal of Ni(II) ions from aqueous medium.
34861277	1	1	theme	TiO2	328:331	arg1	immobilization					333:346	TiO2 immobilization	328:346	TiO2 immobilization	328:346	In this study, organo-funtionalization of sodium-alginate has been carried out using phenylsemicarbazide as modifier to graft N, O-donor atoms containing functional groups (amino-carbamate moieties) to offer novel support for TiO2 immobilization.
34861277	1	2	theme	sodium-alginate	144:158	arg1	organo-funtionalization					117:139	organo-funtionalization	117:139	organo-funtionalization of sodium-alginate	117:158	In this study, organo-funtionalization of sodium-alginate has been carried out using phenylsemicarbazide as modifier to graft N, O-donor atoms containing functional groups (amino-carbamate moieties) to offer novel support for TiO2 immobilization.
34861277	7	3	theme	Batch	1027:1031	arg1	experiments					1038:1048	Batch mode experiments	1027:1048	Batch mode experiments	1027:1048	Batch mode experiments were conducted for optimization of pH and sorbent dose.
34861277	6	4	theme	sites	980:984	arg1	chains					920:925	linear chains	913:925	linear chains of alginate embedded additional chelating functional sites which enhanced sorption and selectivity	913:1024	Organic functionalization based on aminocarbamate (OCONHNH2) moieties on linear chains of alginate embedded additional chelating functional sites which enhanced sorption and selectivity.
34861277	8	5	theme	kinetic	1128:1134	arg1	studies					1154:1160	Equilibrium sorption, kinetic and thermodynamic studies	1106:1160	studies	1154:1160	Equilibrium sorption, kinetic and thermodynamic studies were performed to pattern the nature of sorption.
34861277	6	6	theme	aminocarbamate	875:888	arg1	moieties					901:908	aminocarbamate (OCONHNH2) moieties	875:908	aminocarbamate (OCONHNH2) moieties on linear chains of alginate embedded additional chelating functional sites which enhanced sorption and selectivity	875:1024	Organic functionalization based on aminocarbamate (OCONHNH2) moieties on linear chains of alginate embedded additional chelating functional sites which enhanced sorption and selectivity.
34861277	2	7	theme	Hybrid	349:354	arg1	composite					356:364	Hybrid composite	349:364	Hybrid composite made of aminocarbamated alginate, carboxymethyl chitosan (CMC) and titanium oxide TiO2 (MCA-TiO2)	349:462	Hybrid composite made of aminocarbamated alginate, carboxymethyl chitosan (CMC) and titanium oxide TiO2 (MCA-TiO2) was prepared for the promising adsorptive remediation of Ni(II).
34861277	6	8	theme	functional	969:978	arg1	sites					980:984	alginate embedded additional chelating functional sites	930:984	alginate embedded additional chelating functional sites which enhanced sorption and selectivity	930:1024	Organic functionalization based on aminocarbamate (OCONHNH2) moieties on linear chains of alginate embedded additional chelating functional sites which enhanced sorption and selectivity.
34861277	0	9	theme	aqueous	86:92	arg1	medium					94:99	aqueous medium	86:99	aqueous medium	86:99	Modified alginate-chitosan-TiO2 composites for adsorptive removal of Ni(II) ions from aqueous medium.
34861277	4	10	theme	sorption	739:746	arg1	efficiency					748:757	highest sorption efficiency	731:757	highest sorption efficiency	731:757	The optimization of TiO2 to modified alginate mass ratio was carried out and hydrogel beads with TiO2/MCA mass ratio of 10.0% (2MCA-TiO2) revealed highest sorption efficiency.
34861277	7	11	theme	sorbent	1092:1098	arg1	optimization					1069:1080	optimization	1069:1080	optimization of pH and sorbent dose	1069:1103	Batch mode experiments were conducted for optimization of pH and sorbent dose.
34861277	4	12	theme	%	708:708	arg1	ratio					695:699	TiO2/MCA mass ratio	681:699	TiO2/MCA mass ratio of 10.0% (2MCA-TiO2)	681:720	The optimization of TiO2 to modified alginate mass ratio was carried out and hydrogel beads with TiO2/MCA mass ratio of 10.0% (2MCA-TiO2) revealed highest sorption efficiency.
34861277	1	13	theme	functional	256:265	arg1	moieties					291:298	amino-carbamate moieties	275:298	amino-carbamate moieties	275:298	In this study, organo-funtionalization of sodium-alginate has been carried out using phenylsemicarbazide as modifier to graft N, O-donor atoms containing functional groups (amino-carbamate moieties) to offer novel support for TiO2 immobilization.
34861277	1	13	theme	functional	256:265	arg1	groups					267:272	functional groups	256:272	functional groups (amino-carbamate moieties)	256:299	In this study, organo-funtionalization of sodium-alginate has been carried out using phenylsemicarbazide as modifier to graft N, O-donor atoms containing functional groups (amino-carbamate moieties) to offer novel support for TiO2 immobilization.
34861277	8	14	theme	thermodynamic	1140:1152	arg1	studies					1154:1160	Equilibrium sorption, kinetic and thermodynamic studies	1106:1160	studies	1154:1160	Equilibrium sorption, kinetic and thermodynamic studies were performed to pattern the nature of sorption.
34861277	4	15	with	beads	670:674	arg1	ratio					695:699	TiO2/MCA mass ratio	681:699	TiO2/MCA mass ratio of 10.0% (2MCA-TiO2)	681:720	The optimization of TiO2 to modified alginate mass ratio was carried out and hydrogel beads with TiO2/MCA mass ratio of 10.0% (2MCA-TiO2) revealed highest sorption efficiency.
34861277	6	16	theme	additional	948:957	arg1	sites					980:984	alginate embedded additional chelating functional sites	930:984	alginate embedded additional chelating functional sites which enhanced sorption and selectivity	930:1024	Organic functionalization based on aminocarbamate (OCONHNH2) moieties on linear chains of alginate embedded additional chelating functional sites which enhanced sorption and selectivity.
34861277	5	17	theme	hydrogel	810:817	arg1	beads					819:823	hydrogel beads	810:823	hydrogel beads	810:823	The produced sorbents were adapted in the form of hydrogel beads for operation.
34861277	11	18	theme	sorption	1397:1404	arg1	capacity					1406:1413	Maximum sorption capacity	1389:1413	Maximum sorption capacity	1389:1413	Maximum sorption capacity was evaluated as 229 mg/g at 298 K and pH = 6.0.
34861277	11	18	theme	sorption	1397:1404	arg1	229 mg/g					1432:1439	229 mg/g	1432:1439	229 mg/g	1432:1439	Maximum sorption capacity was evaluated as 229 mg/g at 298 K and pH = 6.0.
34861277	7	19	theme	pH	1085:1086	arg1	optimization					1069:1080	optimization	1069:1080	optimization of pH and sorbent dose	1069:1103	Batch mode experiments were conducted for optimization of pH and sorbent dose.
34861277	8	20	theme	sorption	1118:1125	arg1	studies					1154:1160	Equilibrium sorption, kinetic and thermodynamic studies	1106:1160	studies	1154:1160	Equilibrium sorption, kinetic and thermodynamic studies were performed to pattern the nature of sorption.
34861277	4	21	theme	highest	731:737	arg1	efficiency					748:757	highest sorption efficiency	731:757	highest sorption efficiency	731:757	The optimization of TiO2 to modified alginate mass ratio was carried out and hydrogel beads with TiO2/MCA mass ratio of 10.0% (2MCA-TiO2) revealed highest sorption efficiency.
34861277	2	22	theme	carboxymethyl	400:412	arg1	CMC					424:426	CMC	424:426	CMC	424:426	Hybrid composite made of aminocarbamated alginate, carboxymethyl chitosan (CMC) and titanium oxide TiO2 (MCA-TiO2) was prepared for the promising adsorptive remediation of Ni(II).
34861277	2	22	theme	carboxymethyl	400:412	arg1	chitosan					414:421	carboxymethyl chitosan	400:421	carboxymethyl chitosan (CMC)	400:427	Hybrid composite made of aminocarbamated alginate, carboxymethyl chitosan (CMC) and titanium oxide TiO2 (MCA-TiO2) was prepared for the promising adsorptive remediation of Ni(II).
34861277	2	23	theme	adsorptive	495:504	arg1	remediation					506:516	the promising adsorptive remediation	481:516	the promising adsorptive remediation of Ni(II)	481:526	Hybrid composite made of aminocarbamated alginate, carboxymethyl chitosan (CMC) and titanium oxide TiO2 (MCA-TiO2) was prepared for the promising adsorptive remediation of Ni(II).
34861277	6	24	theme	Organic	840:846	arg1	functionalization					848:864	Organic functionalization	840:864	Organic functionalization	840:864	Organic functionalization based on aminocarbamate (OCONHNH2) moieties on linear chains of alginate embedded additional chelating functional sites which enhanced sorption and selectivity.
34861277	0	25	theme	Modified	0:7	arg1	composites					32:41	Modified alginate-chitosan-TiO2 composites	0:41	Modified alginate-chitosan-TiO2 composites	0:41	Modified alginate-chitosan-TiO2 composites for adsorptive removal of Ni(II) ions from aqueous medium.
34861277	7	26	dep	pH	1085:1086	arg1	dose					1100:1103	dose	1100:1103	dose	1100:1103	Batch mode experiments were conducted for optimization of pH and sorbent dose.
34861277	4	27	theme	mass	630:633	arg1	ratio					635:639	modified alginate mass ratio	612:639	modified alginate mass ratio	612:639	The optimization of TiO2 to modified alginate mass ratio was carried out and hydrogel beads with TiO2/MCA mass ratio of 10.0% (2MCA-TiO2) revealed highest sorption efficiency.
34861277	0	28	theme	alginate-chitosan-TiO2	9:30	arg1	composites					32:41	Modified alginate-chitosan-TiO2 composites	0:41	Modified alginate-chitosan-TiO2 composites	0:41	Modified alginate-chitosan-TiO2 composites for adsorptive removal of Ni(II) ions from aqueous medium.
34861277	9	29	with	agreement	1244:1252	arg1	PSORE					1296:1300	pseudo-second order rate expression (PSORE)	1259:1301	pseudo-second order rate expression (PSORE)	1259:1301	Kinetic data was found in close agreement with pseudo-second order rate expression (PSORE).
34861277	2	30	theme	oxide	442:446	arg1	MCA-TiO2					454:461	MCA-TiO2	454:461	MCA-TiO2	454:461	Hybrid composite made of aminocarbamated alginate, carboxymethyl chitosan (CMC) and titanium oxide TiO2 (MCA-TiO2) was prepared for the promising adsorptive remediation of Ni(II).
34861277	2	30	theme	oxide	442:446	arg1	TiO2					448:451	titanium oxide TiO2	433:451	titanium oxide TiO2 (MCA-TiO2)	433:462	Hybrid composite made of aminocarbamated alginate, carboxymethyl chitosan (CMC) and titanium oxide TiO2 (MCA-TiO2) was prepared for the promising adsorptive remediation of Ni(II).
34861277	6	31	theme	embedded	939:946	arg1	sites					980:984	alginate embedded additional chelating functional sites	930:984	alginate embedded additional chelating functional sites which enhanced sorption and selectivity	930:1024	Organic functionalization based on aminocarbamate (OCONHNH2) moieties on linear chains of alginate embedded additional chelating functional sites which enhanced sorption and selectivity.
34861277	11	32	theme	Maximum	1389:1395	arg1	capacity					1406:1413	Maximum sorption capacity	1389:1413	Maximum sorption capacity	1389:1413	Maximum sorption capacity was evaluated as 229 mg/g at 298 K and pH = 6.0.
34861277	11	32	theme	Maximum	1389:1395	arg1	229 mg/g					1432:1439	229 mg/g	1432:1439	229 mg/g	1432:1439	Maximum sorption capacity was evaluated as 229 mg/g at 298 K and pH = 6.0.
34861277	9	33	theme	order	1273:1277	arg1	PSORE					1296:1300	pseudo-second order rate expression (PSORE)	1259:1301	pseudo-second order rate expression (PSORE)	1259:1301	Kinetic data was found in close agreement with pseudo-second order rate expression (PSORE).
34861277	10	34	theme	Isothermal	1304:1313	arg1	data					1336:1339	Isothermal equilibrium sorption data	1304:1339	Isothermal equilibrium sorption data	1304:1339	Isothermal equilibrium sorption data was well fitted with Langmuir adsorption model.
34861277	2	35	theme	aminocarbamated	374:388	arg1	alginate					390:397	aminocarbamated alginate	374:397	aminocarbamated alginate	374:397	Hybrid composite made of aminocarbamated alginate, carboxymethyl chitosan (CMC) and titanium oxide TiO2 (MCA-TiO2) was prepared for the promising adsorptive remediation of Ni(II).
34861277	2	36	theme	titanium	433:440	arg1	MCA-TiO2					454:461	MCA-TiO2	454:461	MCA-TiO2	454:461	Hybrid composite made of aminocarbamated alginate, carboxymethyl chitosan (CMC) and titanium oxide TiO2 (MCA-TiO2) was prepared for the promising adsorptive remediation of Ni(II).
34861277	2	36	theme	titanium	433:440	arg1	TiO2					448:451	titanium oxide TiO2	433:451	titanium oxide TiO2 (MCA-TiO2)	433:462	Hybrid composite made of aminocarbamated alginate, carboxymethyl chitosan (CMC) and titanium oxide TiO2 (MCA-TiO2) was prepared for the promising adsorptive remediation of Ni(II).
34861277	1	37	theme	amino-carbamate	275:289	arg1	moieties					291:298	amino-carbamate moieties	275:298	amino-carbamate moieties	275:298	In this study, organo-funtionalization of sodium-alginate has been carried out using phenylsemicarbazide as modifier to graft N, O-donor atoms containing functional groups (amino-carbamate moieties) to offer novel support for TiO2 immobilization.
34861277	1	37	theme	amino-carbamate	275:289	arg1	groups					267:272	functional groups	256:272	functional groups (amino-carbamate moieties)	256:299	In this study, organo-funtionalization of sodium-alginate has been carried out using phenylsemicarbazide as modifier to graft N, O-donor atoms containing functional groups (amino-carbamate moieties) to offer novel support for TiO2 immobilization.
34861277	9	38	theme	Kinetic	1212:1218	arg1	data					1220:1223	Kinetic data	1212:1223	Kinetic data	1212:1223	Kinetic data was found in close agreement with pseudo-second order rate expression (PSORE).
34861277	10	39	theme	sorption	1327:1334	arg1	data					1336:1339	Isothermal equilibrium sorption data	1304:1339	Isothermal equilibrium sorption data	1304:1339	Isothermal equilibrium sorption data was well fitted with Langmuir adsorption model.
34861277	4	40	theme	hydrogel	661:668	arg1	beads					670:674	hydrogel beads	661:674	hydrogel beads with TiO2/MCA mass ratio of 10.0% (2MCA-TiO2)	661:720	The optimization of TiO2 to modified alginate mass ratio was carried out and hydrogel beads with TiO2/MCA mass ratio of 10.0% (2MCA-TiO2) revealed highest sorption efficiency.
34861277	4	41	theme	TiO2	604:607	arg1	optimization					588:599	The optimization	584:599	The optimization of TiO2 to modified alginate mass ratio	584:639	The optimization of TiO2 to modified alginate mass ratio was carried out and hydrogel beads with TiO2/MCA mass ratio of 10.0% (2MCA-TiO2) revealed highest sorption efficiency.
34861277	8	42	theme	sorption	1202:1209	arg1	nature					1192:1197	the nature	1188:1197	the nature of sorption	1188:1209	Equilibrium sorption, kinetic and thermodynamic studies were performed to pattern the nature of sorption.
34861277	9	43	located	found	1229:1233	arg1	agreement					1244:1252	close agreement	1238:1252	close agreement with pseudo-second order rate expression (PSORE)	1238:1301	Kinetic data was found in close agreement with pseudo-second order rate expression (PSORE).
34861277	9	43	located	found	1229:1233	arg2	data					1220:1223	Kinetic data	1212:1223	Kinetic data	1212:1223	Kinetic data was found in close agreement with pseudo-second order rate expression (PSORE).
34861277	4	44	theme	alginate	621:628	arg1	ratio					635:639	modified alginate mass ratio	612:639	modified alginate mass ratio	612:639	The optimization of TiO2 to modified alginate mass ratio was carried out and hydrogel beads with TiO2/MCA mass ratio of 10.0% (2MCA-TiO2) revealed highest sorption efficiency.
34861277	0	45	theme	adsorptive	47:56	arg1	removal					58:64	adsorptive removal	47:64	adsorptive removal of Ni(II) ions from aqueous medium	47:99	Modified alginate-chitosan-TiO2 composites for adsorptive removal of Ni(II) ions from aqueous medium.
34861277	6	46	theme	linear	913:918	arg1	chains					920:925	linear chains	913:925	linear chains of alginate embedded additional chelating functional sites which enhanced sorption and selectivity	913:1024	Organic functionalization based on aminocarbamate (OCONHNH2) moieties on linear chains of alginate embedded additional chelating functional sites which enhanced sorption and selectivity.
34861277	4	47	theme	modified	612:619	arg1	ratio					635:639	modified alginate mass ratio	612:639	modified alginate mass ratio	612:639	The optimization of TiO2 to modified alginate mass ratio was carried out and hydrogel beads with TiO2/MCA mass ratio of 10.0% (2MCA-TiO2) revealed highest sorption efficiency.
34861277	0	48	theme	Ni	69:70	arg1	ions					76:79	Ni(II) ions	69:79	Ni(II) ions	69:79	Modified alginate-chitosan-TiO2 composites for adsorptive removal of Ni(II) ions from aqueous medium.
34861277	2	49	theme	Ni	521:522	arg1	remediation					506:516	the promising adsorptive remediation	481:516	the promising adsorptive remediation of Ni(II)	481:526	Hybrid composite made of aminocarbamated alginate, carboxymethyl chitosan (CMC) and titanium oxide TiO2 (MCA-TiO2) was prepared for the promising adsorptive remediation of Ni(II).
34861277	6	50	from	moieties	901:908	arg1	chains					920:925	linear chains	913:925	linear chains of alginate embedded additional chelating functional sites which enhanced sorption and selectivity	913:1024	Organic functionalization based on aminocarbamate (OCONHNH2) moieties on linear chains of alginate embedded additional chelating functional sites which enhanced sorption and selectivity.
34861277	6	51	theme	alginate	930:937	arg1	sites					980:984	alginate embedded additional chelating functional sites	930:984	alginate embedded additional chelating functional sites which enhanced sorption and selectivity	930:1024	Organic functionalization based on aminocarbamate (OCONHNH2) moieties on linear chains of alginate embedded additional chelating functional sites which enhanced sorption and selectivity.
34861277	4	52	theme	TiO2/MCA	681:688	arg1	ratio					695:699	TiO2/MCA mass ratio	681:699	TiO2/MCA mass ratio of 10.0% (2MCA-TiO2)	681:720	The optimization of TiO2 to modified alginate mass ratio was carried out and hydrogel beads with TiO2/MCA mass ratio of 10.0% (2MCA-TiO2) revealed highest sorption efficiency.
34861277	9	53	theme	expression	1284:1293	arg1	PSORE					1296:1300	pseudo-second order rate expression (PSORE)	1259:1301	pseudo-second order rate expression (PSORE)	1259:1301	Kinetic data was found in close agreement with pseudo-second order rate expression (PSORE).
34861277	1	54	theme	novel	310:314	arg1	support					316:322	novel support	310:322	novel support for TiO2 immobilization	310:346	In this study, organo-funtionalization of sodium-alginate has been carried out using phenylsemicarbazide as modifier to graft N, O-donor atoms containing functional groups (amino-carbamate moieties) to offer novel support for TiO2 immobilization.
34861277	7	55	theme	mode	1033:1036	arg1	experiments					1038:1048	Batch mode experiments	1027:1048	Batch mode experiments	1027:1048	Batch mode experiments were conducted for optimization of pH and sorbent dose.
34861277	9	56	theme	close	1238:1242	arg1	agreement					1244:1252	close agreement	1238:1252	close agreement with pseudo-second order rate expression (PSORE)	1238:1301	Kinetic data was found in close agreement with pseudo-second order rate expression (PSORE).
34861277	9	57	theme	rate	1279:1282	arg1	PSORE					1296:1300	pseudo-second order rate expression (PSORE)	1259:1301	pseudo-second order rate expression (PSORE)	1259:1301	Kinetic data was found in close agreement with pseudo-second order rate expression (PSORE).
34861277	8	58	theme	Equilibrium	1106:1116	arg1	studies					1154:1160	Equilibrium sorption, kinetic and thermodynamic studies	1106:1160	studies	1154:1160	Equilibrium sorption, kinetic and thermodynamic studies were performed to pattern the nature of sorption.
34861277	0	59	from	medium	94:99	arg1	removal					58:64	adsorptive removal	47:64	adsorptive removal of Ni(II) ions from aqueous medium	47:99	Modified alginate-chitosan-TiO2 composites for adsorptive removal of Ni(II) ions from aqueous medium.
34861277	10	60	theme	equilibrium	1315:1325	arg1	data					1336:1339	Isothermal equilibrium sorption data	1304:1339	Isothermal equilibrium sorption data	1304:1339	Isothermal equilibrium sorption data was well fitted with Langmuir adsorption model.
34861277	1	61	theme	graft	222:226	arg1	N					228:228	graft N	222:228	graft N	222:228	In this study, organo-funtionalization of sodium-alginate has been carried out using phenylsemicarbazide as modifier to graft N, O-donor atoms containing functional groups (amino-carbamate moieties) to offer novel support for TiO2 immobilization.
34861277	0	62	dep	composites	32:41	arg1	removal					58:64	adsorptive removal	47:64	adsorptive removal of Ni(II) ions from aqueous medium	47:99	Modified alginate-chitosan-TiO2 composites for adsorptive removal of Ni(II) ions from aqueous medium.
34861277	10	63	theme	Langmuir	1362:1369	arg1	adsorption					1371:1380	Langmuir adsorption	1362:1380	Langmuir adsorption model	1362:1386	Isothermal equilibrium sorption data was well fitted with Langmuir adsorption model.
34861277	4	64	theme	mass	690:693	arg1	ratio					695:699	TiO2/MCA mass ratio	681:699	TiO2/MCA mass ratio of 10.0% (2MCA-TiO2)	681:720	The optimization of TiO2 to modified alginate mass ratio was carried out and hydrogel beads with TiO2/MCA mass ratio of 10.0% (2MCA-TiO2) revealed highest sorption efficiency.
34861277	2	65	theme	promising	485:493	arg1	remediation					506:516	the promising adsorptive remediation	481:516	the promising adsorptive remediation of Ni(II)	481:526	Hybrid composite made of aminocarbamated alginate, carboxymethyl chitosan (CMC) and titanium oxide TiO2 (MCA-TiO2) was prepared for the promising adsorptive remediation of Ni(II).
34861277	9	66	theme	pseudo-second	1259:1271	arg1	PSORE					1296:1300	pseudo-second order rate expression (PSORE)	1259:1301	pseudo-second order rate expression (PSORE)	1259:1301	Kinetic data was found in close agreement with pseudo-second order rate expression (PSORE).
34861277	1	67	contain	containing	245:254	arg2	groups					267:272	functional groups	256:272	functional groups (amino-carbamate moieties)	256:299	In this study, organo-funtionalization of sodium-alginate has been carried out using phenylsemicarbazide as modifier to graft N, O-donor atoms containing functional groups (amino-carbamate moieties) to offer novel support for TiO2 immobilization.
34861277	1	67	contain	containing	245:254	arg1	atoms					239:243	O-donor atoms	231:243	O-donor atoms containing functional groups (amino-carbamate moieties)	231:299	In this study, organo-funtionalization of sodium-alginate has been carried out using phenylsemicarbazide as modifier to graft N, O-donor atoms containing functional groups (amino-carbamate moieties) to offer novel support for TiO2 immobilization.
34861277	1	67	contain	containing	245:254	arg2	moieties					291:298	amino-carbamate moieties	275:298	amino-carbamate moieties	275:298	In this study, organo-funtionalization of sodium-alginate has been carried out using phenylsemicarbazide as modifier to graft N, O-donor atoms containing functional groups (amino-carbamate moieties) to offer novel support for TiO2 immobilization.
34861277	5	68	theme	beads	819:823	arg1	form					802:805	the form	798:805	the form of hydrogel beads for operation	798:837	The produced sorbents were adapted in the form of hydrogel beads for operation.
34861277	10	69	theme	adsorption	1371:1380	arg1	model					1382:1386	Langmuir adsorption model	1362:1386	Langmuir adsorption model	1362:1386	Isothermal equilibrium sorption data was well fitted with Langmuir adsorption model.
34861277	6	70	theme	chelating	959:967	arg1	sites					980:984	alginate embedded additional chelating functional sites	930:984	alginate embedded additional chelating functional sites which enhanced sorption and selectivity	930:1024	Organic functionalization based on aminocarbamate (OCONHNH2) moieties on linear chains of alginate embedded additional chelating functional sites which enhanced sorption and selectivity.
34861277	6	71	theme	OCONHNH2	891:898	arg1	moieties					901:908	aminocarbamate (OCONHNH2) moieties	875:908	aminocarbamate (OCONHNH2) moieties on linear chains of alginate embedded additional chelating functional sites which enhanced sorption and selectivity	875:1024	Organic functionalization based on aminocarbamate (OCONHNH2) moieties on linear chains of alginate embedded additional chelating functional sites which enhanced sorption and selectivity.
34861277	5	72	theme	produced	764:771	arg1	sorbents					773:780	The produced sorbents	760:780	The produced sorbents	760:780	The produced sorbents were adapted in the form of hydrogel beads for operation.
34861277	1	73	theme	O-donor	231:237	arg1	atoms					239:243	O-donor atoms	231:243	O-donor atoms containing functional groups (amino-carbamate moieties)	231:299	In this study, organo-funtionalization of sodium-alginate has been carried out using phenylsemicarbazide as modifier to graft N, O-donor atoms containing functional groups (amino-carbamate moieties) to offer novel support for TiO2 immobilization.
35011343	6	0	theme	enantiomer	1318:1327	arg1	reversal					1343:1350	enantiomer elution-order reversal	1318:1350	enantiomer elution-order reversal	1318:1350	In an IPA/MeOH mixture, enantiomer elution-order reversal was observed depending on the eluent composition.
35011343	7	1	theme	amylose-based	1573:1585	arg1	columns					1587:1593	amylose-based columns	1573:1593	amylose-based columns	1573:1593	Furthermore, in eluent mixtures, enantioselectivity depends on the direction from which the composition of the eluent is approached, regardless of the eluent pair used on amylose-based columns.
35011343	7	2	theme	eluent	1418:1423	arg1	mixtures					1425:1432	eluent mixtures	1418:1432	eluent mixtures	1418:1432	Furthermore, in eluent mixtures, enantioselectivity depends on the direction from which the composition of the eluent is approached, regardless of the eluent pair used on amylose-based columns.
35011343	3	3	located	observed	854:861	arg2	>					843:843	Rs > 10	840:846	Rs > 10	840:846	Although on several cases extremely high resolutions (Rs > 10) were observed for certain compounds, among the tested conditions only Chiralcel OJ-H column with MeOH was successful for baseline-separation of all investigated drugs.
35011343	3	3	located	observed	854:861	arg2	resolutions					827:837	extremely high resolutions	812:837	extremely high resolutions (Rs > 10)	812:847	Although on several cases extremely high resolutions (Rs > 10) were observed for certain compounds, among the tested conditions only Chiralcel OJ-H column with MeOH was successful for baseline-separation of all investigated drugs.
35011343	3	3	located	observed	854:861	arg1	cases					806:810	several cases	798:810	several cases	798:810	Although on several cases extremely high resolutions (Rs > 10) were observed for certain compounds, among the tested conditions only Chiralcel OJ-H column with MeOH was successful for baseline-separation of all investigated drugs.
35011343	8	4	theme	alcohols	1621:1628	arg1	mixture					1604:1610	a mixture	1602:1610	a mixture of polar alcohols not only the selectivities	1602:1655	Using a mixture of polar alcohols not only the selectivities but the enantiomer elution order can also be fine-tuned on Chiralpak AD column, which opens up the possibility of a new type of chiral screening strategy.
35011343	0	5	theme	Stationary	86:95	arg1	Phases					97:102	Polysaccharide-Type Stationary Phases	66:102	Polysaccharide-Type Stationary Phases	66:102	Comparative Chiral Separation of Thalidomide Class of Drugs Using Polysaccharide-Type Stationary Phases with Emphasis on Elution Order and Hysteresis in Polar Organic Mode.
35011343	1	6	theme	neat	440:443	arg1	methanol					445:452	neat methanol	440:452	neat methanol (MeOH)	440:459	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	6	theme	neat	440:443	arg1	acetonitrile					518:529	acetonitrile	518:529	acetonitrile (ACN)	518:535	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	6	theme	neat	440:443	arg1	MeOH					455:458	MeOH	455:458	MeOH	455:458	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	6	theme	neat	440:443	arg1	phases					561:566	polar organic mobile phases	540:566	polar organic mobile phases	540:566	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	6	theme	neat	440:443	arg1	2-propanol					497:506	2-propanol	497:506	2-propanol (IPA)	497:512	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	6	theme	neat	440:443	arg1	1-propanol					478:487	1-propanol	478:487	1-propanol (PROP)	478:494	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	6	theme	neat	440:443	arg1	ethanol					462:468	ethanol	462:468	ethanol (EtOH)	462:475	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	8	7	theme	screening	1792:1800	arg1	strategy					1802:1809	chiral screening strategy	1785:1809	chiral screening strategy	1785:1809	Using a mixture of polar alcohols not only the selectivities but the enantiomer elution order can also be fine-tuned on Chiralpak AD column, which opens up the possibility of a new type of chiral screening strategy.
35011343	5	8	theme	different	1212:1220	arg1	mixtures					1229:1236	different eluent mixtures	1212:1236	different eluent mixtures	1212:1236	Reversal of elution order and hysteresis of retention and enantioselectivity were further investigated using different eluent mixtures on Chiralpak AD, Chiralcel OD and Lux Amylose-2 column.
35011343	5	9	theme	hysteresis	1133:1142	arg1	Reversal					1103:1110	Reversal	1103:1110	Reversal of elution order and hysteresis of retention and enantioselectivity	1103:1178	Reversal of elution order and hysteresis of retention and enantioselectivity were further investigated using different eluent mixtures on Chiralpak AD, Chiralcel OD and Lux Amylose-2 column.
35011343	8	10	theme	strategy	1802:1809	arg1	type					1777:1780	a new type	1771:1780	a new type of chiral screening strategy	1771:1809	Using a mixture of polar alcohols not only the selectivities but the enantiomer elution order can also be fine-tuned on Chiralpak AD column, which opens up the possibility of a new type of chiral screening strategy.
35011343	3	11	theme	Chiralcel	919:927	arg1	column					934:939	only Chiralcel OJ-H column	914:939	only Chiralcel OJ-H column with MeOH	914:949	Although on several cases extremely high resolutions (Rs > 10) were observed for certain compounds, among the tested conditions only Chiralcel OJ-H column with MeOH was successful for baseline-separation of all investigated drugs.
35011343	5	12	theme	eluent	1222:1227	arg1	mixtures					1229:1236	different eluent mixtures	1212:1236	different eluent mixtures	1212:1236	Reversal of elution order and hysteresis of retention and enantioselectivity were further investigated using different eluent mixtures on Chiralpak AD, Chiralcel OD and Lux Amylose-2 column.
35011343	6	13	theme	IPA/MeOH	1300:1307	arg1	mixture					1309:1315	an IPA/MeOH mixture	1297:1315	an IPA/MeOH mixture	1297:1315	In an IPA/MeOH mixture, enantiomer elution-order reversal was observed depending on the eluent composition.
35011343	1	14	dep	phases	350:355	arg1	phases					350:355	five different polysaccharide-type stationary phases	304:355	five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H)	304:432	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	14	dep	phases	350:355	arg1	Amylose-2					390:398	Lux Amylose-2	386:398	Lux Amylose-2	386:398	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	14	dep	phases	350:355	arg1	OJ-H					428:431	OJ-H	428:431	OJ-H	428:431	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	14	dep	phases	350:355	arg1	AD					368:369	Chiralpak AD	358:369	Chiralpak AD	358:369	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	14	dep	phases	350:355	arg1	Chiralpak					372:380	Chiralpak	372:380	Chiralpak	372:380	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	14	dep	phases	350:355	arg1	Chiralcel					401:409	Chiralcel	401:409	Chiralcel	401:409	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	3	15	theme	certain	867:873	arg1	compounds					875:883	certain compounds	867:883	certain compounds	867:883	Although on several cases extremely high resolutions (Rs > 10) were observed for certain compounds, among the tested conditions only Chiralcel OJ-H column with MeOH was successful for baseline-separation of all investigated drugs.
35011343	3	16	theme	tested	896:901	arg1	conditions					903:912	the tested conditions	892:912	the tested conditions	892:912	Although on several cases extremely high resolutions (Rs > 10) were observed for certain compounds, among the tested conditions only Chiralcel OJ-H column with MeOH was successful for baseline-separation of all investigated drugs.
35011343	2	17	theme	elution	682:688	arg1	hysteresis					745:754	the hysteresis	741:754	the hysteresis of retention and selectivity	741:783	Along with the separation capacity of the applied systems, our study also focuses on the elution sequences, the effect of mobile phase mixtures and the hysteresis of retention and selectivity.
35011343	2	17	theme	elution	682:688	arg1	sequences					690:698	the elution sequences	678:698	the elution sequences	678:698	Along with the separation capacity of the applied systems, our study also focuses on the elution sequences, the effect of mobile phase mixtures and the hysteresis of retention and selectivity.
35011343	2	17	theme	elution	682:688	arg1	effect					705:710	the effect	701:710	the effect of mobile phase mixtures	701:735	Along with the separation capacity of the applied systems, our study also focuses on the elution sequences, the effect of mobile phase mixtures and the hysteresis of retention and selectivity.
35011343	5	18	theme	retention	1147:1155	arg1	hysteresis					1133:1142	hysteresis	1133:1142	hysteresis	1133:1142	Reversal of elution order and hysteresis of retention and enantioselectivity were further investigated using different eluent mixtures on Chiralpak AD, Chiralcel OD and Lux Amylose-2 column.
35011343	5	18	theme	retention	1147:1155	arg1	order					1123:1127	elution order	1115:1127	elution order	1115:1127	Reversal of elution order and hysteresis of retention and enantioselectivity were further investigated using different eluent mixtures on Chiralpak AD, Chiralcel OD and Lux Amylose-2 column.
35011343	0	19	theme	Elution	121:127	arg1	Order					129:133	Elution Order	121:133	Elution Order	121:133	Comparative Chiral Separation of Thalidomide Class of Drugs Using Polysaccharide-Type Stationary Phases with Emphasis on Elution Order and Hysteresis in Polar Organic Mode.
35011343	5	20	theme	Lux	1272:1274	arg1	column					1286:1291	Lux Amylose-2 column	1272:1291	Lux Amylose-2 column	1272:1291	Reversal of elution order and hysteresis of retention and enantioselectivity were further investigated using different eluent mixtures on Chiralpak AD, Chiralcel OD and Lux Amylose-2 column.
35011343	3	21	theme	investigated	997:1008	arg1	drugs					1010:1014	all investigated drugs	993:1014	all investigated drugs	993:1014	Although on several cases extremely high resolutions (Rs > 10) were observed for certain compounds, among the tested conditions only Chiralcel OJ-H column with MeOH was successful for baseline-separation of all investigated drugs.
35011343	2	22	theme	retention	759:767	arg1	hysteresis					745:754	the hysteresis	741:754	the hysteresis of retention and selectivity	741:783	Along with the separation capacity of the applied systems, our study also focuses on the elution sequences, the effect of mobile phase mixtures and the hysteresis of retention and selectivity.
35011343	2	22	theme	retention	759:767	arg1	sequences					690:698	the elution sequences	678:698	the elution sequences	678:698	Along with the separation capacity of the applied systems, our study also focuses on the elution sequences, the effect of mobile phase mixtures and the hysteresis of retention and selectivity.
35011343	2	22	theme	retention	759:767	arg1	effect					705:710	the effect	701:710	the effect of mobile phase mixtures	701:735	Along with the separation capacity of the applied systems, our study also focuses on the elution sequences, the effect of mobile phase mixtures and the hysteresis of retention and selectivity.
35011343	2	23	theme	mobile	715:720	arg1	mixtures					728:735	mobile phase mixtures	715:735	mobile phase mixtures	715:735	Along with the separation capacity of the applied systems, our study also focuses on the elution sequences, the effect of mobile phase mixtures and the hysteresis of retention and selectivity.
35011343	8	24	theme	enantiomer	1665:1674	arg1	order					1684:1688	the enantiomer elution order	1661:1688	the enantiomer elution order	1661:1688	Using a mixture of polar alcohols not only the selectivities but the enantiomer elution order can also be fine-tuned on Chiralpak AD column, which opens up the possibility of a new type of chiral screening strategy.
35011343	7	25	theme	eluent	1513:1518	arg1	composition					1494:1504	the composition	1490:1504	the composition of the eluent	1490:1518	Furthermore, in eluent mixtures, enantioselectivity depends on the direction from which the composition of the eluent is approached, regardless of the eluent pair used on amylose-based columns.
35011343	1	26	theme	Lux	386:388	arg1	phases					350:355	five different polysaccharide-type stationary phases	304:355	five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H)	304:432	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	26	theme	Lux	386:388	arg1	Amylose-2					390:398	Lux Amylose-2	386:398	Lux Amylose-2	386:398	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	3	27	theme	drugs	1010:1014	arg1	baseline-separation					970:988	baseline-separation	970:988	baseline-separation of all investigated drugs	970:1014	Although on several cases extremely high resolutions (Rs > 10) were observed for certain compounds, among the tested conditions only Chiralcel OJ-H column with MeOH was successful for baseline-separation of all investigated drugs.
35011343	2	28	theme	selectivity	773:783	arg1	hysteresis					745:754	the hysteresis	741:754	the hysteresis of retention and selectivity	741:783	Along with the separation capacity of the applied systems, our study also focuses on the elution sequences, the effect of mobile phase mixtures and the hysteresis of retention and selectivity.
35011343	2	28	theme	selectivity	773:783	arg1	sequences					690:698	the elution sequences	678:698	the elution sequences	678:698	Along with the separation capacity of the applied systems, our study also focuses on the elution sequences, the effect of mobile phase mixtures and the hysteresis of retention and selectivity.
35011343	2	28	theme	selectivity	773:783	arg1	effect					705:710	the effect	701:710	the effect of mobile phase mixtures	701:735	Along with the separation capacity of the applied systems, our study also focuses on the elution sequences, the effect of mobile phase mixtures and the hysteresis of retention and selectivity.
35011343	4	29	theme	elution	1074:1080	arg1	order					1082:1086	elution order	1074:1086	elution order	1074:1086	Chiral selector- and mobile-phase-dependent reversals of elution order were observed.
35011343	0	30	theme	Chiral	12:17	arg1	Separation					19:28	Comparative Chiral Separation	0:28	Comparative Chiral Separation of Thalidomide Class of Drugs Using Polysaccharide-Type Stationary Phases with Emphasis on Elution Order and Hysteresis in Polar Organic Mode.	0:171	Comparative Chiral Separation of Thalidomide Class of Drugs Using Polysaccharide-Type Stationary Phases with Emphasis on Elution Order and Hysteresis in Polar Organic Mode.
35011343	5	31	theme	order	1123:1127	arg1	Reversal					1103:1110	Reversal	1103:1110	Reversal of elution order and hysteresis of retention and enantioselectivity	1103:1178	Reversal of elution order and hysteresis of retention and enantioselectivity were further investigated using different eluent mixtures on Chiralpak AD, Chiralcel OD and Lux Amylose-2 column.
35011343	2	32	theme	separation	608:617	arg1	capacity					619:626	the separation capacity	604:626	the separation capacity of the applied systems	604:649	Along with the separation capacity of the applied systems, our study also focuses on the elution sequences, the effect of mobile phase mixtures and the hysteresis of retention and selectivity.
35011343	3	33	theme	high	822:825	arg1	resolutions					827:837	extremely high resolutions	812:837	extremely high resolutions (Rs > 10)	812:847	Although on several cases extremely high resolutions (Rs > 10) were observed for certain compounds, among the tested conditions only Chiralcel OJ-H column with MeOH was successful for baseline-separation of all investigated drugs.
35011343	3	33	theme	high	822:825	arg1	>					843:843	Rs > 10	840:846	Rs > 10	840:846	Although on several cases extremely high resolutions (Rs > 10) were observed for certain compounds, among the tested conditions only Chiralcel OJ-H column with MeOH was successful for baseline-separation of all investigated drugs.
35011343	0	34	theme	Comparative	0:10	arg1	Separation					19:28	Comparative Chiral Separation	0:28	Comparative Chiral Separation of Thalidomide Class of Drugs Using Polysaccharide-Type Stationary Phases with Emphasis on Elution Order and Hysteresis in Polar Organic Mode.	0:171	Comparative Chiral Separation of Thalidomide Class of Drugs Using Polysaccharide-Type Stationary Phases with Emphasis on Elution Order and Hysteresis in Polar Organic Mode.
35011343	7	35	dep	approached	1523:1532	arg1	used					1565:1568	used	1565:1568	used on amylose-based columns	1565:1593	Furthermore, in eluent mixtures, enantioselectivity depends on the direction from which the composition of the eluent is approached, regardless of the eluent pair used on amylose-based columns.
35011343	3	36	theme	OJ-H	929:932	arg1	column					934:939	only Chiralcel OJ-H column	914:939	only Chiralcel OJ-H column with MeOH	914:949	Although on several cases extremely high resolutions (Rs > 10) were observed for certain compounds, among the tested conditions only Chiralcel OJ-H column with MeOH was successful for baseline-separation of all investigated drugs.
35011343	4	37	theme	Chiral	1017:1022	arg1	reversals					1061:1069	Chiral selector- and mobile-phase-dependent reversals	1017:1069	Chiral selector- and mobile-phase-dependent reversals of elution order	1017:1086	Chiral selector- and mobile-phase-dependent reversals of elution order were observed.
35011343	6	38	theme	eluent	1382:1387	arg1	composition					1389:1399	the eluent composition	1378:1399	the eluent composition	1378:1399	In an IPA/MeOH mixture, enantiomer elution-order reversal was observed depending on the eluent composition.
35011343	3	39	with	column	934:939	arg1	MeOH					946:949	MeOH	946:949	MeOH	946:949	Although on several cases extremely high resolutions (Rs > 10) were observed for certain compounds, among the tested conditions only Chiralcel OJ-H column with MeOH was successful for baseline-separation of all investigated drugs.
35011343	8	40	theme	new	1773:1775	arg1	type					1777:1780	a new type	1771:1780	a new type of chiral screening strategy	1771:1809	Using a mixture of polar alcohols not only the selectivities but the enantiomer elution order can also be fine-tuned on Chiralpak AD column, which opens up the possibility of a new type of chiral screening strategy.
35011343	8	41	theme	elution	1676:1682	arg1	order					1684:1688	the enantiomer elution order	1661:1688	the enantiomer elution order	1661:1688	Using a mixture of polar alcohols not only the selectivities but the enantiomer elution order can also be fine-tuned on Chiralpak AD column, which opens up the possibility of a new type of chiral screening strategy.
35011343	1	42	theme	phthalimide	203:213	arg1	derivatives					215:225	four phthalimide derivatives	198:225	four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast)	198:282	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	42	theme	phthalimide	203:213	arg1	lenalidomide					255:266	lenalidomide	255:266	lenalidomide	255:266	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	42	theme	phthalimide	203:213	arg1	apremilast					272:281	apremilast	272:281	apremilast	272:281	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	42	theme	phthalimide	203:213	arg1	pomalidomide					241:252	pomalidomide	241:252	pomalidomide	241:252	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	42	theme	phthalimide	203:213	arg1	thalidomide					228:238	thalidomide	228:238	thalidomide	228:238	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	4	43	theme	order	1082:1086	arg1	reversals					1061:1069	Chiral selector- and mobile-phase-dependent reversals	1017:1069	Chiral selector- and mobile-phase-dependent reversals of elution order	1017:1086	Chiral selector- and mobile-phase-dependent reversals of elution order were observed.
35011343	5	44	theme	elution	1115:1121	arg1	order					1123:1127	elution order	1115:1127	elution order	1115:1127	Reversal of elution order and hysteresis of retention and enantioselectivity were further investigated using different eluent mixtures on Chiralpak AD, Chiralcel OD and Lux Amylose-2 column.
35011343	5	45	theme	Chiralpak	1241:1249	arg1	AD					1251:1252	Chiralpak AD	1241:1252	Chiralpak AD	1241:1252	Reversal of elution order and hysteresis of retention and enantioselectivity were further investigated using different eluent mixtures on Chiralpak AD, Chiralcel OD and Lux Amylose-2 column.
35011343	3	46	theme	Rs	840:841	arg1	resolutions					827:837	extremely high resolutions	812:837	extremely high resolutions (Rs > 10)	812:847	Although on several cases extremely high resolutions (Rs > 10) were observed for certain compounds, among the tested conditions only Chiralcel OJ-H column with MeOH was successful for baseline-separation of all investigated drugs.
35011343	3	46	theme	Rs	840:841	arg1	>					843:843	Rs > 10	840:846	Rs > 10	840:846	Although on several cases extremely high resolutions (Rs > 10) were observed for certain compounds, among the tested conditions only Chiralcel OJ-H column with MeOH was successful for baseline-separation of all investigated drugs.
35011343	1	47	theme	derivatives	215:225	arg1	enantioseparation					177:193	The enantioseparation	173:193	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast)	173:282	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	0	48	theme	Class	45:49	arg1	Separation					19:28	Comparative Chiral Separation	0:28	Comparative Chiral Separation of Thalidomide Class of Drugs Using Polysaccharide-Type Stationary Phases with Emphasis on Elution Order and Hysteresis in Polar Organic Mode.	0:171	Comparative Chiral Separation of Thalidomide Class of Drugs Using Polysaccharide-Type Stationary Phases with Emphasis on Elution Order and Hysteresis in Polar Organic Mode.
35011343	1	49	theme	different	309:317	arg1	phases					350:355	five different polysaccharide-type stationary phases	304:355	five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H)	304:432	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	49	theme	different	309:317	arg1	Amylose-2					390:398	Lux Amylose-2	386:398	Lux Amylose-2	386:398	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	49	theme	different	309:317	arg1	OJ-H					428:431	OJ-H	428:431	OJ-H	428:431	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	49	theme	different	309:317	arg1	AD					368:369	Chiralpak AD	358:369	Chiralpak AD	358:369	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	49	theme	different	309:317	arg1	Chiralpak					372:380	Chiralpak	372:380	Chiralpak	372:380	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	49	theme	different	309:317	arg1	Chiralcel					401:409	Chiralcel	401:409	Chiralcel	401:409	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	8	50	dep	fine-tuned	1702:1711	arg1	Using					1596:1600	Using	1596:1600	Using a mixture of polar alcohols not only the selectivities	1596:1655	Using a mixture of polar alcohols not only the selectivities but the enantiomer elution order can also be fine-tuned on Chiralpak AD column, which opens up the possibility of a new type of chiral screening strategy.
35011343	4	51	theme	selector-	1024:1032	arg1	reversals					1061:1069	Chiral selector- and mobile-phase-dependent reversals	1017:1069	Chiral selector- and mobile-phase-dependent reversals of elution order	1017:1086	Chiral selector- and mobile-phase-dependent reversals of elution order were observed.
35011343	0	52	theme	Thalidomide	33:43	arg1	Class					45:49	Thalidomide Class	33:49	Thalidomide Class of Drugs Using Polysaccharide-Type Stationary Phases with Emphasis on Elution Order and Hysteresis in Polar Organic Mode	33:170	Comparative Chiral Separation of Thalidomide Class of Drugs Using Polysaccharide-Type Stationary Phases with Emphasis on Elution Order and Hysteresis in Polar Organic Mode.
35011343	1	53	theme	polysaccharide-type	319:337	arg1	phases					350:355	five different polysaccharide-type stationary phases	304:355	five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H)	304:432	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	53	theme	polysaccharide-type	319:337	arg1	Amylose-2					390:398	Lux Amylose-2	386:398	Lux Amylose-2	386:398	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	53	theme	polysaccharide-type	319:337	arg1	OJ-H					428:431	OJ-H	428:431	OJ-H	428:431	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	53	theme	polysaccharide-type	319:337	arg1	AD					368:369	Chiralpak AD	358:369	Chiralpak AD	358:369	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	53	theme	polysaccharide-type	319:337	arg1	Chiralpak					372:380	Chiralpak	372:380	Chiralpak	372:380	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	53	theme	polysaccharide-type	319:337	arg1	Chiralcel					401:409	Chiralcel	401:409	Chiralcel	401:409	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	8	54	theme	chiral	1785:1790	arg1	strategy					1802:1809	chiral screening strategy	1785:1809	chiral screening strategy	1785:1809	Using a mixture of polar alcohols not only the selectivities but the enantiomer elution order can also be fine-tuned on Chiralpak AD column, which opens up the possibility of a new type of chiral screening strategy.
35011343	2	55	theme	systems	643:649	arg1	capacity					619:626	the separation capacity	604:626	the separation capacity of the applied systems	604:649	Along with the separation capacity of the applied systems, our study also focuses on the elution sequences, the effect of mobile phase mixtures and the hysteresis of retention and selectivity.
35011343	8	56	theme	type	1777:1780	arg1	possibility					1756:1766	the possibility	1752:1766	the possibility of a new type of chiral screening strategy	1752:1809	Using a mixture of polar alcohols not only the selectivities but the enantiomer elution order can also be fine-tuned on Chiralpak AD column, which opens up the possibility of a new type of chiral screening strategy.
35011343	0	57	theme	Drugs	54:58	arg1	Class					45:49	Thalidomide Class	33:49	Thalidomide Class of Drugs Using Polysaccharide-Type Stationary Phases with Emphasis on Elution Order and Hysteresis in Polar Organic Mode	33:170	Comparative Chiral Separation of Thalidomide Class of Drugs Using Polysaccharide-Type Stationary Phases with Emphasis on Elution Order and Hysteresis in Polar Organic Mode.
35011343	1	58	theme	stationary	339:348	arg1	phases					350:355	five different polysaccharide-type stationary phases	304:355	five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H)	304:432	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	58	theme	stationary	339:348	arg1	Amylose-2					390:398	Lux Amylose-2	386:398	Lux Amylose-2	386:398	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	58	theme	stationary	339:348	arg1	OJ-H					428:431	OJ-H	428:431	OJ-H	428:431	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	58	theme	stationary	339:348	arg1	AD					368:369	Chiralpak AD	358:369	Chiralpak AD	358:369	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	58	theme	stationary	339:348	arg1	Chiralpak					372:380	Chiralpak	372:380	Chiralpak	372:380	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	58	theme	stationary	339:348	arg1	Chiralcel					401:409	Chiralcel	401:409	Chiralcel	401:409	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	0	59	theme	Organic	159:165	arg1	Mode					167:170	Polar Organic Mode	153:170	Polar Organic Mode	153:170	Comparative Chiral Separation of Thalidomide Class of Drugs Using Polysaccharide-Type Stationary Phases with Emphasis on Elution Order and Hysteresis in Polar Organic Mode.
35011343	4	60	theme	mobile-phase-dependent	1038:1059	arg1	reversals					1061:1069	Chiral selector- and mobile-phase-dependent reversals	1017:1069	Chiral selector- and mobile-phase-dependent reversals of elution order	1017:1086	Chiral selector- and mobile-phase-dependent reversals of elution order were observed.
35011343	2	61	theme	applied	635:641	arg1	systems					643:649	the applied systems	631:649	the applied systems	631:649	Along with the separation capacity of the applied systems, our study also focuses on the elution sequences, the effect of mobile phase mixtures and the hysteresis of retention and selectivity.
35011343	7	62	theme	eluent	1553:1558	arg1	pair					1560:1563	the eluent pair	1549:1563	the eluent pair	1549:1563	Furthermore, in eluent mixtures, enantioselectivity depends on the direction from which the composition of the eluent is approached, regardless of the eluent pair used on amylose-based columns.
35011343	0	63	theme	Polar	153:157	arg1	Mode					167:170	Polar Organic Mode	153:170	Polar Organic Mode	153:170	Comparative Chiral Separation of Thalidomide Class of Drugs Using Polysaccharide-Type Stationary Phases with Emphasis on Elution Order and Hysteresis in Polar Organic Mode.
35011343	8	64	theme	Chiralpak	1716:1724	arg1	column					1729:1734	Chiralpak AD column	1716:1734	Chiralpak AD column	1716:1734	Using a mixture of polar alcohols not only the selectivities but the enantiomer elution order can also be fine-tuned on Chiralpak AD column, which opens up the possibility of a new type of chiral screening strategy.
35011343	2	65	theme	mixtures	728:735	arg1	hysteresis					745:754	the hysteresis	741:754	the hysteresis of retention and selectivity	741:783	Along with the separation capacity of the applied systems, our study also focuses on the elution sequences, the effect of mobile phase mixtures and the hysteresis of retention and selectivity.
35011343	2	65	theme	mixtures	728:735	arg1	sequences					690:698	the elution sequences	678:698	the elution sequences	678:698	Along with the separation capacity of the applied systems, our study also focuses on the elution sequences, the effect of mobile phase mixtures and the hysteresis of retention and selectivity.
35011343	2	65	theme	mixtures	728:735	arg1	effect					705:710	the effect	701:710	the effect of mobile phase mixtures	701:735	Along with the separation capacity of the applied systems, our study also focuses on the elution sequences, the effect of mobile phase mixtures and the hysteresis of retention and selectivity.
35011343	5	66	theme	enantioselectivity	1161:1178	arg1	hysteresis					1133:1142	hysteresis	1133:1142	hysteresis	1133:1142	Reversal of elution order and hysteresis of retention and enantioselectivity were further investigated using different eluent mixtures on Chiralpak AD, Chiralcel OD and Lux Amylose-2 column.
35011343	5	66	theme	enantioselectivity	1161:1178	arg1	order					1123:1127	elution order	1115:1127	elution order	1115:1127	Reversal of elution order and hysteresis of retention and enantioselectivity were further investigated using different eluent mixtures on Chiralpak AD, Chiralcel OD and Lux Amylose-2 column.
35011343	6	67	located	observed	1356:1363	arg2	reversal					1343:1350	enantiomer elution-order reversal	1318:1350	enantiomer elution-order reversal	1318:1350	In an IPA/MeOH mixture, enantiomer elution-order reversal was observed depending on the eluent composition.
35011343	6	67	located	observed	1356:1363	arg1	mixture					1309:1315	an IPA/MeOH mixture	1297:1315	an IPA/MeOH mixture	1297:1315	In an IPA/MeOH mixture, enantiomer elution-order reversal was observed depending on the eluent composition.
35011343	8	68	theme	AD	1726:1727	arg1	column					1729:1734	Chiralpak AD column	1716:1734	Chiralpak AD column	1716:1734	Using a mixture of polar alcohols not only the selectivities but the enantiomer elution order can also be fine-tuned on Chiralpak AD column, which opens up the possibility of a new type of chiral screening strategy.
35011343	2	69	theme	phase	722:726	arg1	mixtures					728:735	mobile phase mixtures	715:735	mobile phase mixtures	715:735	Along with the separation capacity of the applied systems, our study also focuses on the elution sequences, the effect of mobile phase mixtures and the hysteresis of retention and selectivity.
35011343	0	70	from	Emphasis	109:116	arg1	Hysteresis					139:148	Hysteresis	139:148	Hysteresis	139:148	Comparative Chiral Separation of Thalidomide Class of Drugs Using Polysaccharide-Type Stationary Phases with Emphasis on Elution Order and Hysteresis in Polar Organic Mode.
35011343	0	70	from	Emphasis	109:116	arg1	Order					129:133	Elution Order	121:133	Elution Order	121:133	Comparative Chiral Separation of Thalidomide Class of Drugs Using Polysaccharide-Type Stationary Phases with Emphasis on Elution Order and Hysteresis in Polar Organic Mode.
35011343	1	71	theme	polar	540:544	arg1	methanol					445:452	neat methanol	440:452	neat methanol (MeOH)	440:459	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	71	theme	polar	540:544	arg1	acetonitrile					518:529	acetonitrile	518:529	acetonitrile (ACN)	518:535	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	71	theme	polar	540:544	arg1	phases					561:566	polar organic mobile phases	540:566	polar organic mobile phases	540:566	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	71	theme	polar	540:544	arg1	2-propanol					497:506	2-propanol	497:506	2-propanol (IPA)	497:512	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	71	theme	polar	540:544	arg1	1-propanol					478:487	1-propanol	478:487	1-propanol (PROP)	478:494	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	71	theme	polar	540:544	arg1	ethanol					462:468	ethanol	462:468	ethanol (EtOH)	462:475	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	0	72	from	Hysteresis	139:148	arg1	Mode					167:170	Polar Organic Mode	153:170	Polar Organic Mode	153:170	Comparative Chiral Separation of Thalidomide Class of Drugs Using Polysaccharide-Type Stationary Phases with Emphasis on Elution Order and Hysteresis in Polar Organic Mode.
35011343	0	73	theme	Polysaccharide-Type	66:84	arg1	Phases					97:102	Polysaccharide-Type Stationary Phases	66:102	Polysaccharide-Type Stationary Phases	66:102	Comparative Chiral Separation of Thalidomide Class of Drugs Using Polysaccharide-Type Stationary Phases with Emphasis on Elution Order and Hysteresis in Polar Organic Mode.
35011343	7	74	used	used	1565:1568	arg2	composition					1494:1504	the composition	1490:1504	the composition of the eluent	1490:1518	Furthermore, in eluent mixtures, enantioselectivity depends on the direction from which the composition of the eluent is approached, regardless of the eluent pair used on amylose-based columns.
35011343	6	75	theme	elution-order	1329:1341	arg1	reversal					1343:1350	enantiomer elution-order reversal	1318:1350	enantiomer elution-order reversal	1318:1350	In an IPA/MeOH mixture, enantiomer elution-order reversal was observed depending on the eluent composition.
35011343	3	76	theme	several	798:804	arg1	cases					806:810	several cases	798:810	several cases	798:810	Although on several cases extremely high resolutions (Rs > 10) were observed for certain compounds, among the tested conditions only Chiralcel OJ-H column with MeOH was successful for baseline-separation of all investigated drugs.
35011343	8	77	theme	polar	1615:1619	arg1	alcohols					1621:1628	polar alcohols	1615:1628	polar alcohols	1615:1628	Using a mixture of polar alcohols not only the selectivities but the enantiomer elution order can also be fine-tuned on Chiralpak AD column, which opens up the possibility of a new type of chiral screening strategy.
35011343	1	78	theme	organic	546:552	arg1	methanol					445:452	neat methanol	440:452	neat methanol (MeOH)	440:459	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	78	theme	organic	546:552	arg1	acetonitrile					518:529	acetonitrile	518:529	acetonitrile (ACN)	518:535	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	78	theme	organic	546:552	arg1	phases					561:566	polar organic mobile phases	540:566	polar organic mobile phases	540:566	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	78	theme	organic	546:552	arg1	2-propanol					497:506	2-propanol	497:506	2-propanol (IPA)	497:512	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	78	theme	organic	546:552	arg1	1-propanol					478:487	1-propanol	478:487	1-propanol (PROP)	478:494	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	78	theme	organic	546:552	arg1	ethanol					462:468	ethanol	462:468	ethanol (EtOH)	462:475	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	5	79	theme	Amylose-2	1276:1284	arg1	column					1286:1291	Lux Amylose-2 column	1272:1291	Lux Amylose-2 column	1272:1291	Reversal of elution order and hysteresis of retention and enantioselectivity were further investigated using different eluent mixtures on Chiralpak AD, Chiralcel OD and Lux Amylose-2 column.
35011343	1	80	theme	Chiralpak	358:366	arg1	phases					350:355	five different polysaccharide-type stationary phases	304:355	five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H)	304:432	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	80	theme	Chiralpak	358:366	arg1	AD					368:369	Chiralpak AD	358:369	Chiralpak AD	358:369	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	81	dep	derivatives	215:225	arg1	derivatives					215:225	four phthalimide derivatives	198:225	four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast)	198:282	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	81	dep	derivatives	215:225	arg1	apremilast					272:281	apremilast	272:281	apremilast	272:281	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	81	dep	derivatives	215:225	arg1	lenalidomide					255:266	lenalidomide	255:266	lenalidomide	255:266	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	81	dep	derivatives	215:225	arg1	pomalidomide					241:252	pomalidomide	241:252	pomalidomide	241:252	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	81	dep	derivatives	215:225	arg1	thalidomide					228:238	thalidomide	228:238	thalidomide	228:238	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	8	82	dep	mixture	1604:1610	arg1	only					1634:1637	only	1634:1637	only	1634:1637	Using a mixture of polar alcohols not only the selectivities but the enantiomer elution order can also be fine-tuned on Chiralpak AD column, which opens up the possibility of a new type of chiral screening strategy.
35011343	1	83	theme	mobile	554:559	arg1	methanol					445:452	neat methanol	440:452	neat methanol (MeOH)	440:459	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	83	theme	mobile	554:559	arg1	acetonitrile					518:529	acetonitrile	518:529	acetonitrile (ACN)	518:535	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	83	theme	mobile	554:559	arg1	phases					561:566	polar organic mobile phases	540:566	polar organic mobile phases	540:566	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	83	theme	mobile	554:559	arg1	2-propanol					497:506	2-propanol	497:506	2-propanol (IPA)	497:512	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	83	theme	mobile	554:559	arg1	1-propanol					478:487	1-propanol	478:487	1-propanol (PROP)	478:494	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	1	83	theme	mobile	554:559	arg1	ethanol					462:468	ethanol	462:468	ethanol (EtOH)	462:475	The enantioseparation of four phthalimide derivatives (thalidomide, pomalidomide, lenalidomide and apremilast) was investigated on five different polysaccharide-type stationary phases (Chiralpak AD, Chiralpak AS, Lux Amylose-2, Chiralcel OD and Chiralcel OJ-H) using neat methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) as polar organic mobile phases and also in combination.
35011343	0	84	from	Order	129:133	arg1	Mode					167:170	Polar Organic Mode	153:170	Polar Organic Mode	153:170	Comparative Chiral Separation of Thalidomide Class of Drugs Using Polysaccharide-Type Stationary Phases with Emphasis on Elution Order and Hysteresis in Polar Organic Mode.
33592575	5	0	theme	high	714:717	arg1	viscosities					724:734	high peak viscosities	714:734	high peak viscosities	714:734	Varieties with high taste quality had low amylose and protein contents; high peak viscosities and breakdowns; and low hold viscosities, setbacks, and final viscosities.
33592575	6	1	from	%	866:866	arg1	value					904:908	taste value	898:908	taste value	898:908	Protein and combined protein and amylose explained 38.6% and 62.1% of the variation in taste value, respectively.
33592575	0	2	dep	Oryza	108:112	arg1	L.					121:122	Oryza sativa L.	108:122	Oryza sativa L.	108:122	Correlation of taste values with chemical compositions and Rapid Visco Analyser profiles of 36 indica rice (Oryza sativa L.) varieties.
33592575	7	3	theme	variation	1039:1047	arg1	variation					1039:1047	the taste-value variation	1023:1047	the taste-value variation	1023:1047	The RVA profile was affected by protein, amylose, and amylopectin contents and explained 60.5% of the taste-value variation.
33592575	7	3	theme	variation	1039:1047	arg1	%					1018:1018	60.5%	1014:1018	60.5% of the taste-value variation	1014:1047	The RVA profile was affected by protein, amylose, and amylopectin contents and explained 60.5% of the taste-value variation.
33592575	0	4	theme	Analyser	71:78	arg1	profiles					80:87	Rapid Visco Analyser profiles	59:87	Rapid Visco Analyser profiles	59:87	Correlation of taste values with chemical compositions and Rapid Visco Analyser profiles of 36 indica rice (Oryza sativa L.) varieties.
33592575	7	5	theme	taste-value	1027:1037	arg1	variation					1039:1047	the taste-value variation	1023:1047	the taste-value variation	1023:1047	The RVA profile was affected by protein, amylose, and amylopectin contents and explained 60.5% of the taste-value variation.
33592575	1	6	theme	Yield	136:140	arg1	topics					219:224	important research topics	200:224	important research topics in indica rice breeding	200:248	Yield, taste quality, and cultivar utilisation improvements are important research topics in indica rice breeding.
33592575	1	6	theme	Yield	136:140	arg1	improvements					183:194	Yield, taste quality, and cultivar utilisation improvements	136:194	Yield, taste quality, and cultivar utilisation improvements	136:194	Yield, taste quality, and cultivar utilisation improvements are important research topics in indica rice breeding.
33592575	1	7	theme	indica	229:234	arg1	breeding					241:248	indica rice breeding	229:248	indica rice breeding	229:248	Yield, taste quality, and cultivar utilisation improvements are important research topics in indica rice breeding.
33592575	5	8	theme	low	680:682	arg1	amylose					684:690	low amylose	680:690	low amylose	680:690	Varieties with high taste quality had low amylose and protein contents; high peak viscosities and breakdowns; and low hold viscosities, setbacks, and final viscosities.
33592575	1	9	theme	rice	236:239	arg1	breeding					241:248	indica rice breeding	229:248	indica rice breeding	229:248	Yield, taste quality, and cultivar utilisation improvements are important research topics in indica rice breeding.
33592575	4	10	theme	sunlight	570:577	arg1	duration					579:586	sunlight duration	570:586	sunlight duration	570:586	Temperature and sunlight duration during grain filling influenced rice cooking quality.
33592575	2	11	theme	evaluation	330:339	arg1	analyser					418:425	taste analyser	412:425	taste analyser	412:425	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	2	11	theme	evaluation	330:339	arg1	composition					367:377	chemical composition	358:377	chemical composition	358:377	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	2	11	theme	evaluation	330:339	arg1	methods					341:347	three taste evaluation methods	318:347	three taste evaluation methods	318:347	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	2	11	theme	evaluation	330:339	arg1	Analyser					392:399	Rapid Visco Analyser	380:399	Rapid Visco Analyser (RVA)	380:405	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	4	12	theme	grain	595:599	arg1	filling					601:607	grain filling	595:607	grain filling	595:607	Temperature and sunlight duration during grain filling influenced rice cooking quality.
33592575	0	13	theme	rice	102:105	arg1	varieties					125:133	36 indica rice (Oryza sativa L.) varieties	92:133	36 indica rice (Oryza sativa L.) varieties	92:133	Correlation of taste values with chemical compositions and Rapid Visco Analyser profiles of 36 indica rice (Oryza sativa L.) varieties.
33592575	0	13	theme	rice	102:105	arg1	Oryza					108:112	Oryza	108:112	Oryza	108:112	Correlation of taste values with chemical compositions and Rapid Visco Analyser profiles of 36 indica rice (Oryza sativa L.) varieties.
33592575	6	14	from	variation	885:893	arg1	value					904:908	taste value	898:908	taste value	898:908	Protein and combined protein and amylose explained 38.6% and 62.1% of the variation in taste value, respectively.
33592575	2	15	theme	Visco	386:390	arg1	methods					341:347	three taste evaluation methods	318:347	three taste evaluation methods	318:347	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	2	15	theme	Visco	386:390	arg1	RVA					402:404	RVA	402:404	RVA	402:404	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	2	15	theme	Visco	386:390	arg1	Analyser					392:399	Rapid Visco Analyser	380:399	Rapid Visco Analyser (RVA)	380:405	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	3	16	theme	indica	482:487	arg1	varieties					489:497	36 indica varieties	479:497	36 indica varieties commonly grown in Yunnan, Sichuan, and Guizhou, China	479:551	We assessed associations among these methods using 36 indica varieties commonly grown in Yunnan, Sichuan, and Guizhou, China.
33592575	4	17	theme	cooking	625:631	arg1	quality					633:639	rice cooking quality	620:639	rice cooking quality	620:639	Temperature and sunlight duration during grain filling influenced rice cooking quality.
33592575	5	18	theme	final	792:796	arg1	viscosities					798:808	final viscosities	792:808	final viscosities	792:808	Varieties with high taste quality had low amylose and protein contents; high peak viscosities and breakdowns; and low hold viscosities, setbacks, and final viscosities.
33592575	0	19	theme	indica	95:100	arg1	varieties					125:133	36 indica rice (Oryza sativa L.) varieties	92:133	36 indica rice (Oryza sativa L.) varieties	92:133	Correlation of taste values with chemical compositions and Rapid Visco Analyser profiles of 36 indica rice (Oryza sativa L.) varieties.
33592575	0	19	theme	indica	95:100	arg1	Oryza					108:112	Oryza	108:112	Oryza	108:112	Correlation of taste values with chemical compositions and Rapid Visco Analyser profiles of 36 indica rice (Oryza sativa L.) varieties.
33592575	6	20	theme	combined	823:830	arg1	protein					832:838	combined protein	823:838	combined protein	823:838	Protein and combined protein and amylose explained 38.6% and 62.1% of the variation in taste value, respectively.
33592575	6	21	from	value	904:908	arg1	%					876:876	62.1%	872:876	62.1% of the variation in taste value	872:908	Protein and combined protein and amylose explained 38.6% and 62.1% of the variation in taste value, respectively.
33592575	6	21	from	value	904:908	arg1	variation					885:893	the variation	881:893	the variation in taste value	881:908	Protein and combined protein and amylose explained 38.6% and 62.1% of the variation in taste value, respectively.
33592575	6	21	from	value	904:908	arg1	%					866:866	38.6%	862:866	38.6%	862:866	Protein and combined protein and amylose explained 38.6% and 62.1% of the variation in taste value, respectively.
33592575	7	22	theme	amylopectin	979:989	arg1	contents					991:998	amylopectin contents	979:998	amylopectin contents	979:998	The RVA profile was affected by protein, amylose, and amylopectin contents and explained 60.5% of the taste-value variation.
33592575	8	23	theme	taste	1085:1089	arg1	evaluation					1091:1100	taste evaluation	1085:1100	taste evaluation of high-quality rice varieties early in the breeding process, which can improve cultivation and marketing potential	1085:1216	This study lays the foundation for taste evaluation of high-quality rice varieties early in the breeding process, which can improve cultivation and marketing potential.
33592575	1	24	theme	taste	143:147	arg1	quality					149:155	taste quality	143:155	taste quality	143:155	Yield, taste quality, and cultivar utilisation improvements are important research topics in indica rice breeding.
33592575	5	25	theme	peak	719:722	arg1	viscosities					724:734	high peak viscosities	714:734	high peak viscosities	714:734	Varieties with high taste quality had low amylose and protein contents; high peak viscosities and breakdowns; and low hold viscosities, setbacks, and final viscosities.
33592575	6	26	theme	taste	898:902	arg1	value					904:908	taste value	898:908	taste value	898:908	Protein and combined protein and amylose explained 38.6% and 62.1% of the variation in taste value, respectively.
33592575	8	27	from	early	1133:1137	arg1	process					1155:1161	the breeding process	1142:1161	the breeding process	1142:1161	This study lays the foundation for taste evaluation of high-quality rice varieties early in the breeding process, which can improve cultivation and marketing potential.
33592575	1	28	theme	quality	149:155	arg1	topics					219:224	important research topics	200:224	important research topics in indica rice breeding	200:248	Yield, taste quality, and cultivar utilisation improvements are important research topics in indica rice breeding.
33592575	1	28	theme	quality	149:155	arg1	improvements					183:194	Yield, taste quality, and cultivar utilisation improvements	136:194	Yield, taste quality, and cultivar utilisation improvements	136:194	Yield, taste quality, and cultivar utilisation improvements are important research topics in indica rice breeding.
33592575	5	29	theme	low	756:758	arg1	viscosities					765:775	low hold viscosities	756:775	low hold viscosities	756:775	Varieties with high taste quality had low amylose and protein contents; high peak viscosities and breakdowns; and low hold viscosities, setbacks, and final viscosities.
33592575	5	30	contain	had	676:678	arg1	Varieties					642:650	Varieties	642:650	Varieties with high taste quality	642:674	Varieties with high taste quality had low amylose and protein contents; high peak viscosities and breakdowns; and low hold viscosities, setbacks, and final viscosities.
33592575	5	30	contain	had	676:678	arg2	viscosities					765:775	low hold viscosities	756:775	low hold viscosities	756:775	Varieties with high taste quality had low amylose and protein contents; high peak viscosities and breakdowns; and low hold viscosities, setbacks, and final viscosities.
33592575	5	30	contain	had	676:678	arg2	contents					704:711	protein contents	696:711	protein contents	696:711	Varieties with high taste quality had low amylose and protein contents; high peak viscosities and breakdowns; and low hold viscosities, setbacks, and final viscosities.
33592575	5	30	contain	had	676:678	arg2	amylose					684:690	low amylose	680:690	low amylose	680:690	Varieties with high taste quality had low amylose and protein contents; high peak viscosities and breakdowns; and low hold viscosities, setbacks, and final viscosities.
33592575	5	30	contain	had	676:678	arg2	viscosities					724:734	high peak viscosities	714:734	high peak viscosities	714:734	Varieties with high taste quality had low amylose and protein contents; high peak viscosities and breakdowns; and low hold viscosities, setbacks, and final viscosities.
33592575	8	31	theme	breeding	1146:1153	arg1	process					1155:1161	the breeding process	1142:1161	the breeding process	1142:1161	This study lays the foundation for taste evaluation of high-quality rice varieties early in the breeding process, which can improve cultivation and marketing potential.
33592575	2	32	theme	relative	275:282	arg1	effectiveness					284:296	relative effectiveness	275:296	relative effectiveness	275:296	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	4	33	theme	rice	620:623	arg1	quality					633:639	rice cooking quality	620:639	rice cooking quality	620:639	Temperature and sunlight duration during grain filling influenced rice cooking quality.
33592575	0	34	theme	values	21:26	arg1	Correlation					0:10	Correlation	0:10	Correlation of taste values with chemical compositions and Rapid Visco Analyser profiles of 36 indica rice (Oryza sativa L.) varieties.	0:134	Correlation of taste values with chemical compositions and Rapid Visco Analyser profiles of 36 indica rice (Oryza sativa L.) varieties.
33592575	5	35	theme	high	657:660	arg1	quality					668:674	high taste quality	657:674	high taste quality	657:674	Varieties with high taste quality had low amylose and protein contents; high peak viscosities and breakdowns; and low hold viscosities, setbacks, and final viscosities.
33592575	8	36	theme	high-quality	1105:1116	arg1	varieties					1123:1131	high-quality rice varieties	1105:1131	high-quality rice varieties early in the breeding process, which can improve cultivation and marketing potential	1105:1216	This study lays the foundation for taste evaluation of high-quality rice varieties early in the breeding process, which can improve cultivation and marketing potential.
33592575	8	37	theme	varieties	1123:1131	arg1	evaluation					1091:1100	taste evaluation	1085:1100	taste evaluation of high-quality rice varieties early in the breeding process, which can improve cultivation and marketing potential	1085:1216	This study lays the foundation for taste evaluation of high-quality rice varieties early in the breeding process, which can improve cultivation and marketing potential.
33592575	0	38	theme	taste	15:19	arg1	values					21:26	taste values	15:26	taste values	15:26	Correlation of taste values with chemical compositions and Rapid Visco Analyser profiles of 36 indica rice (Oryza sativa L.) varieties.
33592575	6	39	theme	variation	885:893	arg1	%					876:876	62.1%	872:876	62.1% of the variation in taste value	872:908	Protein and combined protein and amylose explained 38.6% and 62.1% of the variation in taste value, respectively.
33592575	6	39	theme	variation	885:893	arg1	variation					885:893	the variation	881:893	the variation in taste value	881:908	Protein and combined protein and amylose explained 38.6% and 62.1% of the variation in taste value, respectively.
33592575	6	39	theme	variation	885:893	arg1	%					866:866	38.6%	862:866	38.6%	862:866	Protein and combined protein and amylose explained 38.6% and 62.1% of the variation in taste value, respectively.
33592575	5	40	theme	taste	662:666	arg1	quality					668:674	high taste quality	657:674	high taste quality	657:674	Varieties with high taste quality had low amylose and protein contents; high peak viscosities and breakdowns; and low hold viscosities, setbacks, and final viscosities.
33592575	2	41	theme	taste	324:328	arg1	analyser					418:425	taste analyser	412:425	taste analyser	412:425	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	2	41	theme	taste	324:328	arg1	composition					367:377	chemical composition	358:377	chemical composition	358:377	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	2	41	theme	taste	324:328	arg1	methods					341:347	three taste evaluation methods	318:347	three taste evaluation methods	318:347	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	2	41	theme	taste	324:328	arg1	Analyser					392:399	Rapid Visco Analyser	380:399	Rapid Visco Analyser (RVA)	380:405	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	1	42	theme	cultivar	162:169	arg1	utilisation					171:181	cultivar utilisation	162:181	cultivar utilisation	162:181	Yield, taste quality, and cultivar utilisation improvements are important research topics in indica rice breeding.
33592575	0	43	theme	chemical	33:40	arg1	compositions					42:53	chemical compositions	33:53	chemical compositions	33:53	Correlation of taste values with chemical compositions and Rapid Visco Analyser profiles of 36 indica rice (Oryza sativa L.) varieties.
33592575	2	44	theme	Rapid	380:384	arg1	methods					341:347	three taste evaluation methods	318:347	three taste evaluation methods	318:347	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	2	44	theme	Rapid	380:384	arg1	RVA					402:404	RVA	402:404	RVA	402:404	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	2	44	theme	Rapid	380:384	arg1	Analyser					392:399	Rapid Visco Analyser	380:399	Rapid Visco Analyser (RVA)	380:405	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	1	45	theme	utilisation	171:181	arg1	topics					219:224	important research topics	200:224	important research topics in indica rice breeding	200:248	Yield, taste quality, and cultivar utilisation improvements are important research topics in indica rice breeding.
33592575	1	45	theme	utilisation	171:181	arg1	improvements					183:194	Yield, taste quality, and cultivar utilisation improvements	136:194	Yield, taste quality, and cultivar utilisation improvements	136:194	Yield, taste quality, and cultivar utilisation improvements are important research topics in indica rice breeding.
33592575	5	46	with	Varieties	642:650	arg1	quality					668:674	high taste quality	657:674	high taste quality	657:674	Varieties with high taste quality had low amylose and protein contents; high peak viscosities and breakdowns; and low hold viscosities, setbacks, and final viscosities.
33592575	8	47	theme	rice	1118:1121	arg1	varieties					1123:1131	high-quality rice varieties	1105:1131	high-quality rice varieties early in the breeding process, which can improve cultivation and marketing potential	1105:1216	This study lays the foundation for taste evaluation of high-quality rice varieties early in the breeding process, which can improve cultivation and marketing potential.
33592575	5	48	theme	hold	760:763	arg1	viscosities					765:775	low hold viscosities	756:775	low hold viscosities	756:775	Varieties with high taste quality had low amylose and protein contents; high peak viscosities and breakdowns; and low hold viscosities, setbacks, and final viscosities.
33592575	5	49	theme	protein	696:702	arg1	contents					704:711	protein contents	696:711	protein contents	696:711	Varieties with high taste quality had low amylose and protein contents; high peak viscosities and breakdowns; and low hold viscosities, setbacks, and final viscosities.
33592575	2	50	theme	taste	412:416	arg1	methods					341:347	three taste evaluation methods	318:347	three taste evaluation methods	318:347	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	2	50	theme	taste	412:416	arg1	analyser					418:425	taste analyser	412:425	taste analyser	412:425	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	0	51	theme	varieties	125:133	arg1	profiles					80:87	Rapid Visco Analyser profiles	59:87	Rapid Visco Analyser profiles	59:87	Correlation of taste values with chemical compositions and Rapid Visco Analyser profiles of 36 indica rice (Oryza sativa L.) varieties.
33592575	0	51	theme	varieties	125:133	arg1	compositions					42:53	chemical compositions	33:53	chemical compositions	33:53	Correlation of taste values with chemical compositions and Rapid Visco Analyser profiles of 36 indica rice (Oryza sativa L.) varieties.
33592575	8	52	from	process	1155:1161	arg1	early					1133:1137	early	1133:1137	early	1133:1137	This study lays the foundation for taste evaluation of high-quality rice varieties early in the breeding process, which can improve cultivation and marketing potential.
33592575	8	53	theme	marketing	1198:1206	arg1	potential					1208:1216	marketing potential	1198:1216	marketing potential	1198:1216	This study lays the foundation for taste evaluation of high-quality rice varieties early in the breeding process, which can improve cultivation and marketing potential.
33592575	8	54	theme	early	1133:1137	arg1	varieties					1123:1131	high-quality rice varieties	1105:1131	high-quality rice varieties early in the breeding process, which can improve cultivation and marketing potential	1105:1216	This study lays the foundation for taste evaluation of high-quality rice varieties early in the breeding process, which can improve cultivation and marketing potential.
33592575	1	55	from	topics	219:224	arg1	breeding					241:248	indica rice breeding	229:248	indica rice breeding	229:248	Yield, taste quality, and cultivar utilisation improvements are important research topics in indica rice breeding.
33592575	0	56	with	Correlation	0:10	arg1	profiles					80:87	Rapid Visco Analyser profiles	59:87	Rapid Visco Analyser profiles	59:87	Correlation of taste values with chemical compositions and Rapid Visco Analyser profiles of 36 indica rice (Oryza sativa L.) varieties.
33592575	0	56	with	Correlation	0:10	arg1	compositions					42:53	chemical compositions	33:53	chemical compositions	33:53	Correlation of taste values with chemical compositions and Rapid Visco Analyser profiles of 36 indica rice (Oryza sativa L.) varieties.
33592575	2	57	dep	effectiveness	284:296	arg1	the					271:273	the	271:273	the	271:273	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	6	58	from	%	876:876	arg1	value					904:908	taste value	898:908	taste value	898:908	Protein and combined protein and amylose explained 38.6% and 62.1% of the variation in taste value, respectively.
33592575	1	59	theme	important	200:208	arg1	topics					219:224	important research topics	200:224	important research topics in indica rice breeding	200:248	Yield, taste quality, and cultivar utilisation improvements are important research topics in indica rice breeding.
33592575	1	59	theme	important	200:208	arg1	improvements					183:194	Yield, taste quality, and cultivar utilisation improvements	136:194	Yield, taste quality, and cultivar utilisation improvements	136:194	Yield, taste quality, and cultivar utilisation improvements are important research topics in indica rice breeding.
33592575	0	60	theme	Visco	65:69	arg1	profiles					80:87	Rapid Visco Analyser profiles	59:87	Rapid Visco Analyser profiles	59:87	Correlation of taste values with chemical compositions and Rapid Visco Analyser profiles of 36 indica rice (Oryza sativa L.) varieties.
33592575	7	61	theme	RVA	929:931	arg1	profile					933:939	The RVA profile	925:939	The RVA profile	925:939	The RVA profile was affected by protein, amylose, and amylopectin contents and explained 60.5% of the taste-value variation.
33592575	2	62	theme	chemical	358:365	arg1	composition					367:377	chemical composition	358:377	chemical composition	358:377	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	2	62	theme	chemical	358:365	arg1	methods					341:347	three taste evaluation methods	318:347	three taste evaluation methods	318:347	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	1	63	theme	research	210:217	arg1	topics					219:224	important research topics	200:224	important research topics in indica rice breeding	200:248	Yield, taste quality, and cultivar utilisation improvements are important research topics in indica rice breeding.
33592575	1	63	theme	research	210:217	arg1	improvements					183:194	Yield, taste quality, and cultivar utilisation improvements	136:194	Yield, taste quality, and cultivar utilisation improvements	136:194	Yield, taste quality, and cultivar utilisation improvements are important research topics in indica rice breeding.
33592575	0	64	theme	Rapid	59:63	arg1	profiles					80:87	Rapid Visco Analyser profiles	59:87	Rapid Visco Analyser profiles	59:87	Correlation of taste values with chemical compositions and Rapid Visco Analyser profiles of 36 indica rice (Oryza sativa L.) varieties.
33592575	2	65	theme	methods	341:347	arg1	relationship					302:313	relationship	302:313	relationship	302:313	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
33592575	2	65	theme	methods	341:347	arg1	effectiveness					284:296	relative effectiveness	275:296	relative effectiveness	275:296	Herein, we compared the relative effectiveness and relationship of three taste evaluation methods, namely, chemical composition, Rapid Visco Analyser (RVA), and taste analyser.
34127218	8	0	theme	4.23	1052:1055	arg1	factor					1042:1047	a factor	1040:1047	a factor of 4.23 in the ZnO/g-C3N4 interface	1040:1083	The vdW is increased by a factor of 4.23 in the ZnO/g-C3N4 interface.
34127218	9	1	theme	electron-hole	1100:1112	arg1	separation					1114:1123	electron-hole separation	1100:1123	electron-hole separation	1100:1123	This improves electron-hole separation and offers prospective application of the composite in photocatalysis, antibacteria and gas sensing.
34127218	8	2	theme	ZnO/g-C3N4	1064:1073	arg1	interface					1075:1083	the ZnO/g-C3N4 interface	1060:1083	the ZnO/g-C3N4 interface	1060:1083	The vdW is increased by a factor of 4.23 in the ZnO/g-C3N4 interface.
34127218	3	3	theme	composite	520:528	arg1	oxide/g-C3N4					545:556	the composite cellulose/zinc oxide/g-C3N4	516:556	the composite cellulose/zinc oxide/g-C3N4	516:556	Herein, we experimentally and computationally investigated the composite cellulose/zinc oxide/g-C3N4.
34127218	0	4	theme	carbon	106:111	arg1	nitride					113:119	graphitic carbon nitride	96:119	graphitic carbon nitride	96:119	Van der Waals enhanced interfacial interaction in cellulose/zinc oxide nanocomposite coupled by graphitic carbon nitride.
34127218	0	5	theme	graphitic	96:104	arg1	nitride					113:119	graphitic carbon nitride	96:119	graphitic carbon nitride	96:119	Van der Waals enhanced interfacial interaction in cellulose/zinc oxide nanocomposite coupled by graphitic carbon nitride.
34127218	4	6	theme	ultrasonic	671:680	arg1	condition					682:690	ultrasonic condition	671:690	ultrasonic condition	671:690	Hydrothermal synthesis afforded cellulose/ZnO, and then fabricated the ternary composite by adding g-C3N4 under ultrasonic condition.
34127218	2	7	theme	techniques	335:344	arg1	limitations					307:317	limitations	307:317	limitations of experimental techniques in locating hydrogen, requiring large/good crystals and detecting a weak interaction like van der Waals (vdW)	307:454	However, the exploration is of great challenge due to limitations of experimental techniques in locating hydrogen, requiring large/good crystals and detecting a weak interaction like van der Waals (vdW).
34127218	8	8	from	factor	1042:1047	arg1	interface					1075:1083	the ZnO/g-C3N4 interface	1060:1083	the ZnO/g-C3N4 interface	1060:1083	The vdW is increased by a factor of 4.23 in the ZnO/g-C3N4 interface.
34127218	2	9	theme	van	436:438	arg1	vdW					451:453	vdW	451:453	vdW	451:453	However, the exploration is of great challenge due to limitations of experimental techniques in locating hydrogen, requiring large/good crystals and detecting a weak interaction like van der Waals (vdW).
34127218	2	9	theme	van	436:438	arg1	Waals					444:448	van der Waals	436:448	van der Waals (vdW)	436:454	However, the exploration is of great challenge due to limitations of experimental techniques in locating hydrogen, requiring large/good crystals and detecting a weak interaction like van der Waals (vdW).
34127218	2	10	theme	great	284:288	arg1	challenge					290:298	great challenge	284:298	great challenge	284:298	However, the exploration is of great challenge due to limitations of experimental techniques in locating hydrogen, requiring large/good crystals and detecting a weak interaction like van der Waals (vdW).
34127218	9	11	theme	prospective	1136:1146	arg1	application					1148:1158	prospective application	1136:1158	prospective application of the composite in photocatalysis, antibacteria and gas sensing	1136:1223	This improves electron-hole separation and offers prospective application of the composite in photocatalysis, antibacteria and gas sensing.
34127218	2	12	theme	weak	414:417	arg1	interaction					419:429	a weak interaction	412:429	a weak interaction like van der Waals (vdW)	412:454	However, the exploration is of great challenge due to limitations of experimental techniques in locating hydrogen, requiring large/good crystals and detecting a weak interaction like van der Waals (vdW).
34127218	4	13	theme	ternary	630:636	arg1	composite					638:646	the ternary composite	626:646	the ternary composite	626:646	Hydrothermal synthesis afforded cellulose/ZnO, and then fabricated the ternary composite by adding g-C3N4 under ultrasonic condition.
34127218	6	14	theme	experimental	833:844	arg1	findings					846:853	These experimental findings	827:853	These experimental findings	827:853	These experimental findings were corroborated by relativistic DFT calculations.
34127218	0	15	theme	der	4:6	arg1	Waals					8:12	Van der Waals	0:12	Van der Waals	0:12	Van der Waals enhanced interfacial interaction in cellulose/zinc oxide nanocomposite coupled by graphitic carbon nitride.
34127218	7	16	theme	interaction	1003:1013	arg1	contributions					949:961	contributions	949:961	contributions of dative bonds, hydrogen bonds and vdW interaction	949:1013	The interfacial coupling is elaborated as contributions of dative bonds, hydrogen bonds and vdW interaction.
34127218	7	16	theme	interaction	1003:1013	arg1	coupling					923:930	The interfacial coupling	907:930	The interfacial coupling	907:930	The interfacial coupling is elaborated as contributions of dative bonds, hydrogen bonds and vdW interaction.
34127218	2	17	theme	experimental	322:333	arg1	techniques					335:344	experimental techniques	322:344	experimental techniques in locating hydrogen, requiring large/good crystals and detecting a weak interaction like van der Waals (vdW)	322:454	However, the exploration is of great challenge due to limitations of experimental techniques in locating hydrogen, requiring large/good crystals and detecting a weak interaction like van der Waals (vdW).
34127218	7	18	theme	interfacial	911:921	arg1	contributions					949:961	contributions	949:961	contributions of dative bonds, hydrogen bonds and vdW interaction	949:1013	The interfacial coupling is elaborated as contributions of dative bonds, hydrogen bonds and vdW interaction.
34127218	7	18	theme	interfacial	911:921	arg1	coupling					923:930	The interfacial coupling	907:930	The interfacial coupling	907:930	The interfacial coupling is elaborated as contributions of dative bonds, hydrogen bonds and vdW interaction.
34127218	9	19	from	sensing	1217:1223	arg1	application					1148:1158	prospective application	1136:1158	prospective application of the composite in photocatalysis, antibacteria and gas sensing	1136:1223	This improves electron-hole separation and offers prospective application of the composite in photocatalysis, antibacteria and gas sensing.
34127218	0	20	theme	Van	0:2	arg1	Waals					8:12	Van der Waals	0:12	Van der Waals	0:12	Van der Waals enhanced interfacial interaction in cellulose/zinc oxide nanocomposite coupled by graphitic carbon nitride.
34127218	7	21	theme	bonds	989:993	arg1	contributions					949:961	contributions	949:961	contributions of dative bonds, hydrogen bonds and vdW interaction	949:1013	The interfacial coupling is elaborated as contributions of dative bonds, hydrogen bonds and vdW interaction.
34127218	7	21	theme	bonds	989:993	arg1	coupling					923:930	The interfacial coupling	907:930	The interfacial coupling	907:930	The interfacial coupling is elaborated as contributions of dative bonds, hydrogen bonds and vdW interaction.
34127218	9	22	from	composite	1167:1175	arg1	antibacteria					1196:1207	antibacteria	1196:1207	antibacteria	1196:1207	This improves electron-hole separation and offers prospective application of the composite in photocatalysis, antibacteria and gas sensing.
34127218	9	22	from	composite	1167:1175	arg1	sensing					1217:1223	gas sensing	1213:1223	gas sensing	1213:1223	This improves electron-hole separation and offers prospective application of the composite in photocatalysis, antibacteria and gas sensing.
34127218	9	22	from	composite	1167:1175	arg1	photocatalysis					1180:1193	photocatalysis	1180:1193	photocatalysis	1180:1193	This improves electron-hole separation and offers prospective application of the composite in photocatalysis, antibacteria and gas sensing.
34127218	9	23	theme	composite	1167:1175	arg1	application					1148:1158	prospective application	1136:1158	prospective application of the composite in photocatalysis, antibacteria and gas sensing	1136:1223	This improves electron-hole separation and offers prospective application of the composite in photocatalysis, antibacteria and gas sensing.
34127218	2	24	theme	der	440:442	arg1	vdW					451:453	vdW	451:453	vdW	451:453	However, the exploration is of great challenge due to limitations of experimental techniques in locating hydrogen, requiring large/good crystals and detecting a weak interaction like van der Waals (vdW).
34127218	2	24	theme	der	440:442	arg1	Waals					444:448	van der Waals	436:448	van der Waals (vdW)	436:454	However, the exploration is of great challenge due to limitations of experimental techniques in locating hydrogen, requiring large/good crystals and detecting a weak interaction like van der Waals (vdW).
34127218	1	25	theme	biomass	208:214	arg1	composites					216:225	biomass composites	208:225	biomass composites	208:225	In-depth understanding of interfacial property is the key to guiding the synthesis of biomass composites with desired performance.
34127218	1	26	theme	composites	216:225	arg1	synthesis					195:203	the synthesis	191:203	the synthesis of biomass composites with desired performance	191:250	In-depth understanding of interfacial property is the key to guiding the synthesis of biomass composites with desired performance.
34127218	0	27	theme	interfacial	23:33	arg1	interaction					35:45	interfacial interaction	23:45	interfacial interaction	23:45	Van der Waals enhanced interfacial interaction in cellulose/zinc oxide nanocomposite coupled by graphitic carbon nitride.
34127218	4	28	theme	Hydrothermal	559:570	arg1	synthesis					572:580	Hydrothermal synthesis	559:580	Hydrothermal synthesis	559:580	Hydrothermal synthesis afforded cellulose/ZnO, and then fabricated the ternary composite by adding g-C3N4 under ultrasonic condition.
34127218	5	29	theme	ZnO	758:760	arg1	attaching					778:786	attaching	778:786	attaching	778:786	Three components are found to co-exist in the composite, and the ZnO nanoparticle is attaching to cellulose and coupling with g-C3N4.
34127218	5	29	theme	ZnO	758:760	arg1	nanoparticle					762:773	the ZnO nanoparticle	754:773	the ZnO nanoparticle	754:773	Three components are found to co-exist in the composite, and the ZnO nanoparticle is attaching to cellulose and coupling with g-C3N4.
34127218	9	30	from	photocatalysis	1180:1193	arg1	application					1148:1158	prospective application	1136:1158	prospective application of the composite in photocatalysis, antibacteria and gas sensing	1136:1223	This improves electron-hole separation and offers prospective application of the composite in photocatalysis, antibacteria and gas sensing.
34127218	9	31	from	antibacteria	1196:1207	arg1	application					1148:1158	prospective application	1136:1158	prospective application of the composite in photocatalysis, antibacteria and gas sensing	1136:1223	This improves electron-hole separation and offers prospective application of the composite in photocatalysis, antibacteria and gas sensing.
34127218	0	32	theme	cellulose/zinc	50:63	arg1	nanocomposite					71:83	cellulose/zinc oxide nanocomposite	50:83	cellulose/zinc oxide nanocomposite coupled by graphitic carbon nitride	50:119	Van der Waals enhanced interfacial interaction in cellulose/zinc oxide nanocomposite coupled by graphitic carbon nitride.
34127218	1	33	theme	In-depth	122:129	arg1	understanding					131:143	In-depth understanding	122:143	In-depth understanding of interfacial property	122:167	In-depth understanding of interfacial property is the key to guiding the synthesis of biomass composites with desired performance.
34127218	1	33	theme	In-depth	122:129	arg1	key					176:178	key	176:178	key	176:178	In-depth understanding of interfacial property is the key to guiding the synthesis of biomass composites with desired performance.
34127218	1	34	with	synthesis	195:203	arg1	performance					240:250	desired performance	232:250	desired performance	232:250	In-depth understanding of interfacial property is the key to guiding the synthesis of biomass composites with desired performance.
34127218	7	35	theme	vdW	999:1001	arg1	interaction					1003:1013	vdW interaction	999:1013	vdW interaction	999:1013	The interfacial coupling is elaborated as contributions of dative bonds, hydrogen bonds and vdW interaction.
34127218	1	36	theme	property	160:167	arg1	understanding					131:143	In-depth understanding	122:143	In-depth understanding of interfacial property	122:167	In-depth understanding of interfacial property is the key to guiding the synthesis of biomass composites with desired performance.
34127218	1	36	theme	property	160:167	arg1	key					176:178	key	176:178	key	176:178	In-depth understanding of interfacial property is the key to guiding the synthesis of biomass composites with desired performance.
34127218	5	37	with	coupling	805:812	arg1	g-C3N4					819:824	g-C3N4	819:824	g-C3N4	819:824	Three components are found to co-exist in the composite, and the ZnO nanoparticle is attaching to cellulose and coupling with g-C3N4.
34127218	9	38	theme	gas	1213:1215	arg1	sensing					1217:1223	gas sensing	1213:1223	gas sensing	1213:1223	This improves electron-hole separation and offers prospective application of the composite in photocatalysis, antibacteria and gas sensing.
34127218	7	39	theme	hydrogen	980:987	arg1	bonds					989:993	hydrogen bonds	980:993	hydrogen bonds	980:993	The interfacial coupling is elaborated as contributions of dative bonds, hydrogen bonds and vdW interaction.
34127218	1	40	theme	desired	232:238	arg1	performance					240:250	desired performance	232:250	desired performance	232:250	In-depth understanding of interfacial property is the key to guiding the synthesis of biomass composites with desired performance.
34127218	6	41	theme	DFT	889:891	arg1	calculations					893:904	relativistic DFT calculations	876:904	relativistic DFT calculations	876:904	These experimental findings were corroborated by relativistic DFT calculations.
34127218	1	42	theme	interfacial	148:158	arg1	property					160:167	interfacial property	148:167	interfacial property	148:167	In-depth understanding of interfacial property is the key to guiding the synthesis of biomass composites with desired performance.
34127218	7	43	theme	dative	966:971	arg1	bonds					973:977	dative bonds	966:977	dative bonds	966:977	The interfacial coupling is elaborated as contributions of dative bonds, hydrogen bonds and vdW interaction.
34127218	9	44	from	application	1148:1158	arg1	antibacteria					1196:1207	antibacteria	1196:1207	antibacteria	1196:1207	This improves electron-hole separation and offers prospective application of the composite in photocatalysis, antibacteria and gas sensing.
34127218	9	44	from	application	1148:1158	arg1	sensing					1217:1223	gas sensing	1213:1223	gas sensing	1213:1223	This improves electron-hole separation and offers prospective application of the composite in photocatalysis, antibacteria and gas sensing.
34127218	9	44	from	application	1148:1158	arg1	photocatalysis					1180:1193	photocatalysis	1180:1193	photocatalysis	1180:1193	This improves electron-hole separation and offers prospective application of the composite in photocatalysis, antibacteria and gas sensing.
34127218	6	45	theme	relativistic	876:887	arg1	calculations					893:904	relativistic DFT calculations	876:904	relativistic DFT calculations	876:904	These experimental findings were corroborated by relativistic DFT calculations.
34127218	5	46	with	cellulose	791:799	arg1	g-C3N4					819:824	g-C3N4	819:824	g-C3N4	819:824	Three components are found to co-exist in the composite, and the ZnO nanoparticle is attaching to cellulose and coupling with g-C3N4.
34127218	3	47	theme	cellulose/zinc	530:543	arg1	oxide/g-C3N4					545:556	the composite cellulose/zinc oxide/g-C3N4	516:556	the composite cellulose/zinc oxide/g-C3N4	516:556	Herein, we experimentally and computationally investigated the composite cellulose/zinc oxide/g-C3N4.
34127218	7	48	theme	bonds	973:977	arg1	contributions					949:961	contributions	949:961	contributions of dative bonds, hydrogen bonds and vdW interaction	949:1013	The interfacial coupling is elaborated as contributions of dative bonds, hydrogen bonds and vdW interaction.
34127218	7	48	theme	bonds	973:977	arg1	coupling					923:930	The interfacial coupling	907:930	The interfacial coupling	907:930	The interfacial coupling is elaborated as contributions of dative bonds, hydrogen bonds and vdW interaction.
34127218	0	49	theme	oxide	65:69	arg1	nanocomposite					71:83	cellulose/zinc oxide nanocomposite	50:83	cellulose/zinc oxide nanocomposite coupled by graphitic carbon nitride	50:119	Van der Waals enhanced interfacial interaction in cellulose/zinc oxide nanocomposite coupled by graphitic carbon nitride.
34127218	2	50	theme	large/good	378:387	arg1	crystals					389:396	large/good crystals	378:396	large/good crystals	378:396	However, the exploration is of great challenge due to limitations of experimental techniques in locating hydrogen, requiring large/good crystals and detecting a weak interaction like van der Waals (vdW).
32899783	5	0	theme	sheep	938:942	arg1	erythrocyte					944:954	sheep erythrocyte	938:954	sheep erythrocyte	938:954	The anti-inflammatory activity of PEC and the initial compounds was studied using test of histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice.
32899783	5	1	from	mice	993:996	arg1	test					897:900	test	897:900	test of histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice	897:996	The anti-inflammatory activity of PEC and the initial compounds was studied using test of histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice.
32899783	1	2	theme	κ-carrageenan	148:160	arg1	activity					101:108	The immunotropic activity	84:108	The immunotropic activity of polyelectrolyte complexes (PEC) of κ-carrageenan (κ-CGN) and chitosan (CH) of various compositions	84:210	The immunotropic activity of polyelectrolyte complexes (PEC) of κ-carrageenan (κ-CGN) and chitosan (CH) of various compositions was assessed in comparison with the initial polysaccharides in comparable doses.
32899783	0	3	theme	Complexes	73:81	arg1	Activity					29:36	The Comparative Immunotropic Activity	0:36	The Comparative Immunotropic Activity of Carrageenan, Chitosan and Their Complexes	0:81	The Comparative Immunotropic Activity of Carrageenan, Chitosan and Their Complexes.
32899783	4	4	theme	anti-inflammatory	638:654	arg1	cytokines					682:690	pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines	583:690	pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines	583:690	The ability of the PEC to induce the synthesis of pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines in peripheral blood mononuclear cell was determined by the activity of the initial κ-CGN, regardless of their composition.
32899783	4	5	theme	cytokines	682:690	arg1	synthesis					570:578	the synthesis	566:578	the synthesis of pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines	566:690	The ability of the PEC to induce the synthesis of pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines in peripheral blood mononuclear cell was determined by the activity of the initial κ-CGN, regardless of their composition.
32899783	2	6	theme	mass	414:417	arg1	ratios					419:424	CGN mass ratios	410:424	CH: CGN mass ratios of 1:10	406:432	For this, two soluble forms of PEC, with an excess of CH (CH:CGN mass ratios of 10:1) and with an excess of CGN (CH: CGN mass ratios of 1:10) were prepared.
32899783	5	7	theme	immunization-induced	956:975	arg1	inflammation					977:988	histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation	905:988	histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice	905:996	The anti-inflammatory activity of PEC and the initial compounds was studied using test of histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice.
32899783	4	8	theme	blood	706:710	arg1	cell					724:727	peripheral blood mononuclear cell	695:727	peripheral blood mononuclear cell	695:727	The ability of the PEC to induce the synthesis of pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines in peripheral blood mononuclear cell was determined by the activity of the initial κ-CGN, regardless of their composition.
32899783	4	9	theme	tumor	601:605	arg1	TNF-α					626:630	TNF-α	626:630	TNF-α	626:630	The ability of the PEC to induce the synthesis of pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines in peripheral blood mononuclear cell was determined by the activity of the initial κ-CGN, regardless of their composition.
32899783	4	9	theme	tumor	601:605	arg1	factor-α					616:623	tumor necrosis factor-α	601:623	tumor necrosis factor-α (TNF-α)	601:631	The ability of the PEC to induce the synthesis of pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines in peripheral blood mononuclear cell was determined by the activity of the initial κ-CGN, regardless of their composition.
32899783	6	10	theme	polysaccharides	1051:1065	arg1	activity					1011:1018	The highest activity	999:1018	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH,	999:1079	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH, was observed in a histamine-induced exudative inflammation, directly related to the activation of phagocytic cells, i.e., macrophages and neutrophils.
32899783	2	11	theme	CGN	410:412	arg1	ratios					419:424	CGN mass ratios	410:424	CH: CGN mass ratios of 1:10	406:432	For this, two soluble forms of PEC, with an excess of CH (CH:CGN mass ratios of 10:1) and with an excess of CGN (CH: CGN mass ratios of 1:10) were prepared.
32899783	4	12	theme	peripheral	695:704	arg1	cell					724:727	peripheral blood mononuclear cell	695:727	peripheral blood mononuclear cell	695:727	The ability of the PEC to induce the synthesis of pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines in peripheral blood mononuclear cell was determined by the activity of the initial κ-CGN, regardless of their composition.
32899783	2	13	theme	PEC	324:326	arg1	forms					315:319	two soluble forms	303:319	two soluble forms of PEC, with an excess of CH (CH:CGN mass ratios of 10:1) and with an excess of CGN (CH: CGN mass ratios of 1:10)	303:433	For this, two soluble forms of PEC, with an excess of CH (CH:CGN mass ratios of 10:1) and with an excess of CGN (CH: CGN mass ratios of 1:10) were prepared.
32899783	4	14	theme	PEC	552:554	arg1	ability					537:543	The ability	533:543	The ability of the PEC to induce the synthesis of pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines in peripheral blood mononuclear cell	533:727	The ability of the PEC to induce the synthesis of pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines in peripheral blood mononuclear cell was determined by the activity of the initial κ-CGN, regardless of their composition.
32899783	6	15	theme	highest	1003:1009	arg1	activity					1011:1018	The highest activity	999:1018	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH,	999:1079	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH, was observed in a histamine-induced exudative inflammation, directly related to the activation of phagocytic cells, i.e., macrophages and neutrophils.
32899783	2	16	theme	CH	347:348	arg1	excess					337:342	an excess	334:342	an excess of CH (CH:CGN mass ratios of 10:1)	334:377	For this, two soluble forms of PEC, with an excess of CH (CH:CGN mass ratios of 10:1) and with an excess of CGN (CH: CGN mass ratios of 1:10) were prepared.
32899783	4	17	theme	κ-CGN	775:779	arg1	activity					751:758	the activity	747:758	the activity of the initial κ-CGN	747:779	The ability of the PEC to induce the synthesis of pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines in peripheral blood mononuclear cell was determined by the activity of the initial κ-CGN, regardless of their composition.
32899783	5	18	theme	A-	930:931	arg1	inflammation					977:988	histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation	905:988	histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice	905:996	The anti-inflammatory activity of PEC and the initial compounds was studied using test of histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice.
32899783	4	19	theme	necrosis	607:614	arg1	TNF-α					626:630	TNF-α	626:630	TNF-α	626:630	The ability of the PEC to induce the synthesis of pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines in peripheral blood mononuclear cell was determined by the activity of the initial κ-CGN, regardless of their composition.
32899783	4	19	theme	necrosis	607:614	arg1	factor-α					616:623	tumor necrosis factor-α	601:623	tumor necrosis factor-α (TNF-α)	601:631	The ability of the PEC to induce the synthesis of pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines in peripheral blood mononuclear cell was determined by the activity of the initial κ-CGN, regardless of their composition.
32899783	0	20	theme	Comparative	4:14	arg1	Activity					29:36	The Comparative Immunotropic Activity	0:36	The Comparative Immunotropic Activity of Carrageenan, Chitosan and Their Complexes	0:81	The Comparative Immunotropic Activity of Carrageenan, Chitosan and Their Complexes.
32899783	4	21	dep	pro-inflammatory	583:598	arg1	TNF-α					626:630	TNF-α	626:630	TNF-α	626:630	The ability of the PEC to induce the synthesis of pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines in peripheral blood mononuclear cell was determined by the activity of the initial κ-CGN, regardless of their composition.
32899783	4	21	dep	pro-inflammatory	583:598	arg1	factor-α					616:623	tumor necrosis factor-α	601:623	tumor necrosis factor-α (TNF-α)	601:631	The ability of the PEC to induce the synthesis of pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines in peripheral blood mononuclear cell was determined by the activity of the initial κ-CGN, regardless of their composition.
32899783	4	21	dep	pro-inflammatory	583:598	arg1	IL-10					674:678	IL-10	674:678	IL-10	674:678	The ability of the PEC to induce the synthesis of pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines in peripheral blood mononuclear cell was determined by the activity of the initial κ-CGN, regardless of their composition.
32899783	4	21	dep	pro-inflammatory	583:598	arg1	interleukine-10					657:671	interleukine-10	657:671	interleukine-10 (IL-10)	657:679	The ability of the PEC to induce the synthesis of pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines in peripheral blood mononuclear cell was determined by the activity of the initial κ-CGN, regardless of their composition.
32899783	6	22	theme	initial	1043:1049	arg1	CH					1077:1078	CH	1077:1078	CH	1077:1078	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH, was observed in a histamine-induced exudative inflammation, directly related to the activation of phagocytic cells, i.e., macrophages and neutrophils.
32899783	6	22	theme	initial	1043:1049	arg1	κ-CGN					1067:1071	κ-CGN	1067:1071	κ-CGN	1067:1071	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH, was observed in a histamine-induced exudative inflammation, directly related to the activation of phagocytic cells, i.e., macrophages and neutrophils.
32899783	6	22	theme	initial	1043:1049	arg1	polysaccharides					1051:1065	the initial polysaccharides	1039:1065	the initial polysaccharides κ-CGN and CH	1039:1078	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH, was observed in a histamine-induced exudative inflammation, directly related to the activation of phagocytic cells, i.e., macrophages and neutrophils.
32899783	5	23	theme	concanavalin	917:928	arg1	A-					930:931	concanavalin A-	917:931	concanavalin A-	917:931	The anti-inflammatory activity of PEC and the initial compounds was studied using test of histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice.
32899783	5	24	theme	erythrocyte	944:954	arg1	inflammation					977:988	histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation	905:988	histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice	905:996	The anti-inflammatory activity of PEC and the initial compounds was studied using test of histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice.
32899783	1	25	theme	initial	248:254	arg1	polysaccharides					256:270	the initial polysaccharides	244:270	the initial polysaccharides in comparable doses	244:290	The immunotropic activity of polyelectrolyte complexes (PEC) of κ-carrageenan (κ-CGN) and chitosan (CH) of various compositions was assessed in comparison with the initial polysaccharides in comparable doses.
32899783	1	26	from	polysaccharides	256:270	arg1	doses					286:290	comparable doses	275:290	comparable doses	275:290	The immunotropic activity of polyelectrolyte complexes (PEC) of κ-carrageenan (κ-CGN) and chitosan (CH) of various compositions was assessed in comparison with the initial polysaccharides in comparable doses.
32899783	1	27	theme	immunotropic	88:99	arg1	activity					101:108	The immunotropic activity	84:108	The immunotropic activity of polyelectrolyte complexes (PEC) of κ-carrageenan (κ-CGN) and chitosan (CH) of various compositions	84:210	The immunotropic activity of polyelectrolyte complexes (PEC) of κ-carrageenan (κ-CGN) and chitosan (CH) of various compositions was assessed in comparison with the initial polysaccharides in comparable doses.
32899783	1	28	theme	chitosan	174:181	arg1	activity					101:108	The immunotropic activity	84:108	The immunotropic activity of polyelectrolyte complexes (PEC) of κ-carrageenan (κ-CGN) and chitosan (CH) of various compositions	84:210	The immunotropic activity of polyelectrolyte complexes (PEC) of κ-carrageenan (κ-CGN) and chitosan (CH) of various compositions was assessed in comparison with the initial polysaccharides in comparable doses.
32899783	2	29	dep	CH	406:407	arg1	ratios					419:424	CGN mass ratios	410:424	CH: CGN mass ratios of 1:10	406:432	For this, two soluble forms of PEC, with an excess of CH (CH:CGN mass ratios of 10:1) and with an excess of CGN (CH: CGN mass ratios of 1:10) were prepared.
32899783	4	30	theme	pro-inflammatory	583:598	arg1	cytokines					682:690	pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines	583:690	pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines	583:690	The ability of the PEC to induce the synthesis of pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines in peripheral blood mononuclear cell was determined by the activity of the initial κ-CGN, regardless of their composition.
32899783	3	31	theme	PEC	465:467	arg1	ability					454:460	The ability	450:460	The ability of PEC to scavenge NO	450:482	The ability of PEC to scavenge NO depended on the content of the κ-CGN in the PEC.
32899783	2	32	theme	CGN	354:356	arg1	ratios					363:368	CGN mass ratios	354:368	CH:CGN mass ratios of 10:1	351:376	For this, two soluble forms of PEC, with an excess of CH (CH:CGN mass ratios of 10:1) and with an excess of CGN (CH: CGN mass ratios of 1:10) were prepared.
32899783	2	33	theme	mass	358:361	arg1	ratios					363:368	CGN mass ratios	354:368	CH:CGN mass ratios of 10:1	351:376	For this, two soluble forms of PEC, with an excess of CH (CH:CGN mass ratios of 10:1) and with an excess of CGN (CH: CGN mass ratios of 1:10) were prepared.
32899783	6	34	dep	macrophages	1203:1213	arg1	i.e.					1197:1200	i.e.	1197:1200	i.e.	1197:1200	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH, was observed in a histamine-induced exudative inflammation, directly related to the activation of phagocytic cells, i.e., macrophages and neutrophils.
32899783	2	35	theme	1:10	429:432	arg1	ratios					419:424	CGN mass ratios	410:424	CH: CGN mass ratios of 1:10	406:432	For this, two soluble forms of PEC, with an excess of CH (CH:CGN mass ratios of 10:1) and with an excess of CGN (CH: CGN mass ratios of 1:10) were prepared.
32899783	0	36	theme	Carrageenan	41:51	arg1	Activity					29:36	The Comparative Immunotropic Activity	0:36	The Comparative Immunotropic Activity of Carrageenan, Chitosan and Their Complexes	0:81	The Comparative Immunotropic Activity of Carrageenan, Chitosan and Their Complexes.
32899783	1	37	theme	comparable	275:284	arg1	doses					286:290	comparable doses	275:290	comparable doses	275:290	The immunotropic activity of polyelectrolyte complexes (PEC) of κ-carrageenan (κ-CGN) and chitosan (CH) of various compositions was assessed in comparison with the initial polysaccharides in comparable doses.
32899783	6	38	theme	related	1150:1156	arg1	inflammation					1127:1138	a histamine-induced exudative inflammation	1097:1138	a histamine-induced exudative inflammation	1097:1138	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH, was observed in a histamine-induced exudative inflammation, directly related to the activation of phagocytic cells, i.e., macrophages and neutrophils.
32899783	2	39	dep	CH	351:352	arg1	ratios					363:368	CGN mass ratios	354:368	CH:CGN mass ratios of 10:1	351:376	For this, two soluble forms of PEC, with an excess of CH (CH:CGN mass ratios of 10:1) and with an excess of CGN (CH: CGN mass ratios of 1:10) were prepared.
32899783	6	40	located	observed	1085:1092	arg1	inflammation					1127:1138	a histamine-induced exudative inflammation	1097:1138	a histamine-induced exudative inflammation	1097:1138	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH, was observed in a histamine-induced exudative inflammation, directly related to the activation of phagocytic cells, i.e., macrophages and neutrophils.
32899783	6	40	located	observed	1085:1092	arg2	activity					1011:1018	The highest activity	999:1018	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH,	999:1079	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH, was observed in a histamine-induced exudative inflammation, directly related to the activation of phagocytic cells, i.e., macrophages and neutrophils.
32899783	5	41	theme	inflammation	977:988	arg1	test					897:900	test	897:900	test of histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice	897:996	The anti-inflammatory activity of PEC and the initial compounds was studied using test of histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice.
32899783	5	42	theme	PEC	849:851	arg1	activity					837:844	The anti-inflammatory activity	815:844	The anti-inflammatory activity of PEC and the initial compounds	815:877	The anti-inflammatory activity of PEC and the initial compounds was studied using test of histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice.
32899783	5	43	from	test	897:900	arg1	mice					993:996	mice	993:996	mice	993:996	The anti-inflammatory activity of PEC and the initial compounds was studied using test of histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice.
32899783	0	44	theme	Chitosan	54:61	arg1	Activity					29:36	The Comparative Immunotropic Activity	0:36	The Comparative Immunotropic Activity of Carrageenan, Chitosan and Their Complexes	0:81	The Comparative Immunotropic Activity of Carrageenan, Chitosan and Their Complexes.
32899783	4	45	theme	initial	767:773	arg1	κ-CGN					775:779	the initial κ-CGN	763:779	the initial κ-CGN	763:779	The ability of the PEC to induce the synthesis of pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines in peripheral blood mononuclear cell was determined by the activity of the initial κ-CGN, regardless of their composition.
32899783	6	46	theme	cells	1190:1194	arg1	activation					1165:1174	the activation	1161:1174	the activation of phagocytic cells	1161:1194	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH, was observed in a histamine-induced exudative inflammation, directly related to the activation of phagocytic cells, i.e., macrophages and neutrophils.
32899783	5	47	theme	histamine-	905:914	arg1	inflammation					977:988	histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation	905:988	histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice	905:996	The anti-inflammatory activity of PEC and the initial compounds was studied using test of histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice.
32899783	1	48	theme	polyelectrolyte	113:127	arg1	PEC					140:142	PEC	140:142	PEC	140:142	The immunotropic activity of polyelectrolyte complexes (PEC) of κ-carrageenan (κ-CGN) and chitosan (CH) of various compositions was assessed in comparison with the initial polysaccharides in comparable doses.
32899783	1	48	theme	polyelectrolyte	113:127	arg1	complexes					129:137	polyelectrolyte complexes	113:137	polyelectrolyte complexes (PEC)	113:143	The immunotropic activity of polyelectrolyte complexes (PEC) of κ-carrageenan (κ-CGN) and chitosan (CH) of various compositions was assessed in comparison with the initial polysaccharides in comparable doses.
32899783	4	49	theme	mononuclear	712:722	arg1	cell					724:727	peripheral blood mononuclear cell	695:727	peripheral blood mononuclear cell	695:727	The ability of the PEC to induce the synthesis of pro-inflammatory (tumor necrosis factor-α (TNF-α)) and anti-inflammatory (interleukine-10 (IL-10)) cytokines in peripheral blood mononuclear cell was determined by the activity of the initial κ-CGN, regardless of their composition.
32899783	2	50	with	PEC	324:326	arg1	excess					391:396	an excess	388:396	an excess of CGN (CH: CGN mass ratios of 1:10)	388:433	For this, two soluble forms of PEC, with an excess of CH (CH:CGN mass ratios of 10:1) and with an excess of CGN (CH: CGN mass ratios of 1:10) were prepared.
32899783	2	50	with	PEC	324:326	arg1	excess					337:342	an excess	334:342	an excess of CH (CH:CGN mass ratios of 10:1)	334:377	For this, two soluble forms of PEC, with an excess of CH (CH:CGN mass ratios of 10:1) and with an excess of CGN (CH: CGN mass ratios of 1:10) were prepared.
32899783	1	51	theme	complexes	129:137	arg1	activity					101:108	The immunotropic activity	84:108	The immunotropic activity of polyelectrolyte complexes (PEC) of κ-carrageenan (κ-CGN) and chitosan (CH) of various compositions	84:210	The immunotropic activity of polyelectrolyte complexes (PEC) of κ-carrageenan (κ-CGN) and chitosan (CH) of various compositions was assessed in comparison with the initial polysaccharides in comparable doses.
32899783	5	52	theme	initial	861:867	arg1	compounds					869:877	the initial compounds	857:877	the initial compounds	857:877	The anti-inflammatory activity of PEC and the initial compounds was studied using test of histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice.
32899783	6	53	theme	PEC	1023:1025	arg1	activity					1011:1018	The highest activity	999:1018	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH,	999:1079	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH, was observed in a histamine-induced exudative inflammation, directly related to the activation of phagocytic cells, i.e., macrophages and neutrophils.
32899783	6	54	theme	exudative	1117:1125	arg1	inflammation					1127:1138	a histamine-induced exudative inflammation	1097:1138	a histamine-induced exudative inflammation	1097:1138	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH, was observed in a histamine-induced exudative inflammation, directly related to the activation of phagocytic cells, i.e., macrophages and neutrophils.
32899783	5	55	theme	compounds	869:877	arg1	activity					837:844	The anti-inflammatory activity	815:844	The anti-inflammatory activity of PEC and the initial compounds	815:877	The anti-inflammatory activity of PEC and the initial compounds was studied using test of histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice.
32899783	1	56	with	comparison	228:237	arg1	polysaccharides					256:270	the initial polysaccharides	244:270	the initial polysaccharides in comparable doses	244:290	The immunotropic activity of polyelectrolyte complexes (PEC) of κ-carrageenan (κ-CGN) and chitosan (CH) of various compositions was assessed in comparison with the initial polysaccharides in comparable doses.
32899783	1	57	theme	various	191:197	arg1	compositions					199:210	various compositions	191:210	various compositions	191:210	The immunotropic activity of polyelectrolyte complexes (PEC) of κ-carrageenan (κ-CGN) and chitosan (CH) of various compositions was assessed in comparison with the initial polysaccharides in comparable doses.
32899783	6	58	theme	histamine-induced	1099:1115	arg1	inflammation					1127:1138	a histamine-induced exudative inflammation	1097:1138	a histamine-induced exudative inflammation	1097:1138	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH, was observed in a histamine-induced exudative inflammation, directly related to the activation of phagocytic cells, i.e., macrophages and neutrophils.
32899783	3	59	theme	κ-CGN	515:519	arg1	content					500:506	the content	496:506	the content of the κ-CGN in the PEC	496:530	The ability of PEC to scavenge NO depended on the content of the κ-CGN in the PEC.
32899783	6	60	theme	phagocytic	1179:1188	arg1	cells					1190:1194	phagocytic cells	1179:1194	phagocytic cells	1179:1194	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH, was observed in a histamine-induced exudative inflammation, directly related to the activation of phagocytic cells, i.e., macrophages and neutrophils.
32899783	1	61	theme	compositions	199:210	arg1	activity					101:108	The immunotropic activity	84:108	The immunotropic activity of polyelectrolyte complexes (PEC) of κ-carrageenan (κ-CGN) and chitosan (CH) of various compositions	84:210	The immunotropic activity of polyelectrolyte complexes (PEC) of κ-carrageenan (κ-CGN) and chitosan (CH) of various compositions was assessed in comparison with the initial polysaccharides in comparable doses.
32899783	6	62	dep	polysaccharides	1051:1065	arg1	CH					1077:1078	CH	1077:1078	CH	1077:1078	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH, was observed in a histamine-induced exudative inflammation, directly related to the activation of phagocytic cells, i.e., macrophages and neutrophils.
32899783	6	62	dep	polysaccharides	1051:1065	arg1	κ-CGN					1067:1071	κ-CGN	1067:1071	κ-CGN	1067:1071	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH, was observed in a histamine-induced exudative inflammation, directly related to the activation of phagocytic cells, i.e., macrophages and neutrophils.
32899783	6	62	dep	polysaccharides	1051:1065	arg1	polysaccharides					1051:1065	the initial polysaccharides	1039:1065	the initial polysaccharides κ-CGN and CH	1039:1078	The highest activity of PEC, as well as the initial polysaccharides κ-CGN and CH, was observed in a histamine-induced exudative inflammation, directly related to the activation of phagocytic cells, i.e., macrophages and neutrophils.
32899783	2	63	theme	soluble	307:313	arg1	forms					315:319	two soluble forms	303:319	two soluble forms of PEC, with an excess of CH (CH:CGN mass ratios of 10:1) and with an excess of CGN (CH: CGN mass ratios of 1:10)	303:433	For this, two soluble forms of PEC, with an excess of CH (CH:CGN mass ratios of 10:1) and with an excess of CGN (CH: CGN mass ratios of 1:10) were prepared.
32899783	5	64	theme	anti-inflammatory	819:835	arg1	activity					837:844	The anti-inflammatory activity	815:844	The anti-inflammatory activity of PEC and the initial compounds	815:877	The anti-inflammatory activity of PEC and the initial compounds was studied using test of histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice.
32899783	2	65	theme	CGN	401:403	arg1	excess					391:396	an excess	388:396	an excess of CGN (CH: CGN mass ratios of 1:10)	388:433	For this, two soluble forms of PEC, with an excess of CH (CH:CGN mass ratios of 10:1) and with an excess of CGN (CH: CGN mass ratios of 1:10) were prepared.
32899783	0	66	theme	Immunotropic	16:27	arg1	Activity					29:36	The Comparative Immunotropic Activity	0:36	The Comparative Immunotropic Activity of Carrageenan, Chitosan and Their Complexes	0:81	The Comparative Immunotropic Activity of Carrageenan, Chitosan and Their Complexes.
32899783	2	67	theme	10:1	373:376	arg1	ratios					363:368	CGN mass ratios	354:368	CH:CGN mass ratios of 10:1	351:376	For this, two soluble forms of PEC, with an excess of CH (CH:CGN mass ratios of 10:1) and with an excess of CGN (CH: CGN mass ratios of 1:10) were prepared.
32899783	5	68	from	inflammation	977:988	arg1	mice					993:996	mice	993:996	mice	993:996	The anti-inflammatory activity of PEC and the initial compounds was studied using test of histamine-, concanavalin A-, and sheep erythrocyte immunization-induced inflammation in mice.
32899783	3	69	from	content	500:506	arg1	PEC					528:530	the PEC	524:530	the PEC	524:530	The ability of PEC to scavenge NO depended on the content of the κ-CGN in the PEC.
34323712	7	0	theme	reduction	1095:1103	arg1	adsorption					1070:1079	The adsorption	1066:1079	The adsorption of Cr(VI) and reduction of Cr(VI) to Cr(III)	1066:1124	The adsorption of Cr(VI) and reduction of Cr(VI) to Cr(III) contribute to elimination of Cr(VI) species.
34323712	6	1	theme	XPS	960:962	arg1	analysis					964:971	XPS analysis	960:971	XPS analysis	960:971	XPS analysis showed that Cr(VI) was converted to Cr(III) with low toxicity during the adsorption process.
34323712	3	2	theme	imidazoles-MXene	646:661	arg1	composite					670:678	a novel imidazoles-MXene hybrid composite	638:678	a novel imidazoles-MXene hybrid composite	638:678	Based on the characterization results, we demonstrated that a thin layer imidazoles with an ordered chain structure was embedded on the surface of Ti3C2, which resulted in the formation of a novel imidazoles-MXene hybrid composite.
34323712	1	3	theme	imidazoles-MXene	146:161	arg1	composite					170:178	a novel imidazoles-MXene hybrid composite	138:178	a novel imidazoles-MXene hybrid composite	138:178	In this work, a novel imidazoles-MXene hybrid composite, namely polyimidazoles chain overlaying on the surface of MXene (Ti3C2@IMIZ), was prepared by a simple method.
34323712	1	3	theme	imidazoles-MXene	146:161	arg1	chain					203:207	polyimidazoles chain	188:207	polyimidazoles chain overlaying on the surface of MXene (Ti3C2@IMIZ)	188:255	In this work, a novel imidazoles-MXene hybrid composite, namely polyimidazoles chain overlaying on the surface of MXene (Ti3C2@IMIZ), was prepared by a simple method.
34323712	1	4	theme	Ti3C2	245:249	arg1	MXene					238:242	MXene	238:242	MXene (Ti3C2@IMIZ)	238:255	In this work, a novel imidazoles-MXene hybrid composite, namely polyimidazoles chain overlaying on the surface of MXene (Ti3C2@IMIZ), was prepared by a simple method.
34323712	1	4	theme	Ti3C2	245:249	arg1	IMIZ					251:254	Ti3C2@IMIZ	245:254	Ti3C2@IMIZ	245:254	In this work, a novel imidazoles-MXene hybrid composite, namely polyimidazoles chain overlaying on the surface of MXene (Ti3C2@IMIZ), was prepared by a simple method.
34323712	2	5	theme	renewable	429:437	arg1	chitosan					415:422	chitosan	415:422	chitosan	415:422	Through this strategy, imidazoles can be in situ growth on the surface of MXenes via a facile multicomponent reaction using chitosan as a renewable reactant.
34323712	2	5	theme	renewable	429:437	arg1	reactant					439:446	a renewable reactant	427:446	a renewable reactant	427:446	Through this strategy, imidazoles can be in situ growth on the surface of MXenes via a facile multicomponent reaction using chitosan as a renewable reactant.
34323712	9	6	theme	actual	1423:1428	arg1	treatment					1437:1445	actual sewage treatment	1423:1445	actual sewage treatment	1423:1445	The excellent reproducibility suggests that Ti3C2@IMIZ may be a potential candidate for remove of Cr(Ⅵ) in actual sewage treatment.
34323712	5	7	theme	Detailed	819:826	arg1	isotherms					924:932	isotherms	924:932	isotherms	924:932	Detailed adsorption characteristics of Ti3C2@IMIZ including operational factors, adsorption kinetics and isotherms models were investigated.
34323712	5	7	theme	Detailed	819:826	arg1	factors					891:897	operational factors	879:897	operational factors	879:897	Detailed adsorption characteristics of Ti3C2@IMIZ including operational factors, adsorption kinetics and isotherms models were investigated.
34323712	5	7	theme	Detailed	819:826	arg1	kinetics					911:918	adsorption kinetics	900:918	adsorption kinetics	900:918	Detailed adsorption characteristics of Ti3C2@IMIZ including operational factors, adsorption kinetics and isotherms models were investigated.
34323712	5	7	theme	Detailed	819:826	arg1	characteristics					839:853	Detailed adsorption characteristics	819:853	Detailed adsorption characteristics of Ti3C2@IMIZ including operational factors, adsorption kinetics and isotherms models	819:939	Detailed adsorption characteristics of Ti3C2@IMIZ including operational factors, adsorption kinetics and isotherms models were investigated.
34323712	1	8	theme	hybrid	163:168	arg1	composite					170:178	a novel imidazoles-MXene hybrid composite	138:178	a novel imidazoles-MXene hybrid composite	138:178	In this work, a novel imidazoles-MXene hybrid composite, namely polyimidazoles chain overlaying on the surface of MXene (Ti3C2@IMIZ), was prepared by a simple method.
34323712	1	8	theme	hybrid	163:168	arg1	chain					203:207	polyimidazoles chain	188:207	polyimidazoles chain overlaying on the surface of MXene (Ti3C2@IMIZ)	188:255	In this work, a novel imidazoles-MXene hybrid composite, namely polyimidazoles chain overlaying on the surface of MXene (Ti3C2@IMIZ), was prepared by a simple method.
34323712	9	9	from	candidate	1390:1398	arg1	treatment					1437:1445	actual sewage treatment	1423:1445	actual sewage treatment	1423:1445	The excellent reproducibility suggests that Ti3C2@IMIZ may be a potential candidate for remove of Cr(Ⅵ) in actual sewage treatment.
34323712	1	10	theme	@	250:250	arg1	MXene					238:242	MXene	238:242	MXene (Ti3C2@IMIZ)	238:255	In this work, a novel imidazoles-MXene hybrid composite, namely polyimidazoles chain overlaying on the surface of MXene (Ti3C2@IMIZ), was prepared by a simple method.
34323712	1	10	theme	@	250:250	arg1	IMIZ					251:254	Ti3C2@IMIZ	245:254	Ti3C2@IMIZ	245:254	In this work, a novel imidazoles-MXene hybrid composite, namely polyimidazoles chain overlaying on the surface of MXene (Ti3C2@IMIZ), was prepared by a simple method.
34323712	0	11	theme	adsorption	101:110	arg1	properties					112:121	its adsorption properties	97:121	its adsorption properties	97:121	Surface functionalization of MXene with chitosan through in-situ formation of polyimidazoles and its adsorption properties.
34323712	9	12	theme	sewage	1430:1435	arg1	treatment					1437:1445	actual sewage treatment	1423:1445	actual sewage treatment	1423:1445	The excellent reproducibility suggests that Ti3C2@IMIZ may be a potential candidate for remove of Cr(Ⅵ) in actual sewage treatment.
34323712	4	13	theme	Ti3C2	711:715	arg1	IMIZ					717:720	Ti3C2@IMIZ	711:720	Ti3C2@IMIZ for removal environmental pollutants	711:757	The adsorption performance of Ti3C2@IMIZ for removal environmental pollutants was evaluated using heavy metal ions of Cr(Ⅵ) as adsorbate.
34323712	4	14	theme	adsorption	685:694	arg1	performance					696:706	The adsorption performance	681:706	The adsorption performance of Ti3C2@IMIZ for removal environmental pollutants	681:757	The adsorption performance of Ti3C2@IMIZ for removal environmental pollutants was evaluated using heavy metal ions of Cr(Ⅵ) as adsorbate.
34323712	5	15	theme	IMIZ	864:867	arg1	isotherms					924:932	isotherms	924:932	isotherms	924:932	Detailed adsorption characteristics of Ti3C2@IMIZ including operational factors, adsorption kinetics and isotherms models were investigated.
34323712	5	15	theme	IMIZ	864:867	arg1	factors					891:897	operational factors	879:897	operational factors	879:897	Detailed adsorption characteristics of Ti3C2@IMIZ including operational factors, adsorption kinetics and isotherms models were investigated.
34323712	5	15	theme	IMIZ	864:867	arg1	kinetics					911:918	adsorption kinetics	900:918	adsorption kinetics	900:918	Detailed adsorption characteristics of Ti3C2@IMIZ including operational factors, adsorption kinetics and isotherms models were investigated.
34323712	5	15	theme	IMIZ	864:867	arg1	characteristics					839:853	Detailed adsorption characteristics	819:853	Detailed adsorption characteristics of Ti3C2@IMIZ including operational factors, adsorption kinetics and isotherms models	819:939	Detailed adsorption characteristics of Ti3C2@IMIZ including operational factors, adsorption kinetics and isotherms models were investigated.
34323712	3	16	theme	hybrid	663:668	arg1	composite					670:678	a novel imidazoles-MXene hybrid composite	638:678	a novel imidazoles-MXene hybrid composite	638:678	Based on the characterization results, we demonstrated that a thin layer imidazoles with an ordered chain structure was embedded on the surface of Ti3C2, which resulted in the formation of a novel imidazoles-MXene hybrid composite.
34323712	7	17	dep	Cr	1084:1085	arg1	VI					1087:1088	VI	1087:1088	VI	1087:1088	The adsorption of Cr(VI) and reduction of Cr(VI) to Cr(III) contribute to elimination of Cr(VI) species.
34323712	5	18	theme	adsorption	900:909	arg1	kinetics					911:918	adsorption kinetics	900:918	adsorption kinetics	900:918	Detailed adsorption characteristics of Ti3C2@IMIZ including operational factors, adsorption kinetics and isotherms models were investigated.
34323712	3	19	theme	composite	670:678	arg1	formation					625:633	the formation	621:633	the formation of a novel imidazoles-MXene hybrid composite	621:678	Based on the characterization results, we demonstrated that a thin layer imidazoles with an ordered chain structure was embedded on the surface of Ti3C2, which resulted in the formation of a novel imidazoles-MXene hybrid composite.
34323712	7	20	theme	Cr	1155:1156	arg1	elimination					1140:1150	elimination	1140:1150	elimination of Cr(VI) species	1140:1168	The adsorption of Cr(VI) and reduction of Cr(VI) to Cr(III) contribute to elimination of Cr(VI) species.
34323712	2	21	dep	in	332:333	arg1	situ					335:338	situ	335:338	situ	335:338	Through this strategy, imidazoles can be in situ growth on the surface of MXenes via a facile multicomponent reaction using chitosan as a renewable reactant.
34323712	3	22	theme	novel	640:644	arg1	composite					670:678	a novel imidazoles-MXene hybrid composite	638:678	a novel imidazoles-MXene hybrid composite	638:678	Based on the characterization results, we demonstrated that a thin layer imidazoles with an ordered chain structure was embedded on the surface of Ti3C2, which resulted in the formation of a novel imidazoles-MXene hybrid composite.
34323712	4	23	theme	metal	785:789	arg1	ions					791:794	heavy metal ions	779:794	heavy metal ions of Cr(Ⅵ)	779:803	The adsorption performance of Ti3C2@IMIZ for removal environmental pollutants was evaluated using heavy metal ions of Cr(Ⅵ) as adsorbate.
34323712	0	24	theme	Surface	0:6	arg1	functionalization					8:24	Surface functionalization	0:24	Surface functionalization of MXene with chitosan through in-situ formation of polyimidazoles and its adsorption properties.	0:122	Surface functionalization of MXene with chitosan through in-situ formation of polyimidazoles and its adsorption properties.
34323712	0	25	theme	polyimidazoles	78:91	arg1	formation					65:73	in-situ formation	57:73	in-situ formation of polyimidazoles	57:91	Surface functionalization of MXene with chitosan through in-situ formation of polyimidazoles and its adsorption properties.
34323712	0	25	theme	polyimidazoles	78:91	arg1	properties					112:121	its adsorption properties	97:121	its adsorption properties	97:121	Surface functionalization of MXene with chitosan through in-situ formation of polyimidazoles and its adsorption properties.
34323712	4	26	theme	Cr	799:800	arg1	ions					791:794	heavy metal ions	779:794	heavy metal ions of Cr(Ⅵ)	779:803	The adsorption performance of Ti3C2@IMIZ for removal environmental pollutants was evaluated using heavy metal ions of Cr(Ⅵ) as adsorbate.
34323712	3	27	theme	layer	516:520	arg1	embedded					569:576	embedded	569:576	embedded	569:576	Based on the characterization results, we demonstrated that a thin layer imidazoles with an ordered chain structure was embedded on the surface of Ti3C2, which resulted in the formation of a novel imidazoles-MXene hybrid composite.
34323712	3	27	theme	layer	516:520	arg1	imidazoles					522:531	a thin layer imidazoles	509:531	a thin layer imidazoles with an ordered chain structure	509:563	Based on the characterization results, we demonstrated that a thin layer imidazoles with an ordered chain structure was embedded on the surface of Ti3C2, which resulted in the formation of a novel imidazoles-MXene hybrid composite.
34323712	6	28	with	Cr	1009:1010	arg1	toxicity					1026:1033	low toxicity	1022:1033	low toxicity	1022:1033	XPS analysis showed that Cr(VI) was converted to Cr(III) with low toxicity during the adsorption process.
34323712	2	29	theme	multicomponent	385:398	arg1	reaction					400:407	a facile multicomponent reaction	376:407	a facile multicomponent reaction using chitosan as a renewable reactant	376:446	Through this strategy, imidazoles can be in situ growth on the surface of MXenes via a facile multicomponent reaction using chitosan as a renewable reactant.
34323712	5	30	dep	factors	891:897	arg1	models					934:939	models	934:939	models	934:939	Detailed adsorption characteristics of Ti3C2@IMIZ including operational factors, adsorption kinetics and isotherms models were investigated.
34323712	1	31	theme	polyimidazoles	188:201	arg1	composite					170:178	a novel imidazoles-MXene hybrid composite	138:178	a novel imidazoles-MXene hybrid composite	138:178	In this work, a novel imidazoles-MXene hybrid composite, namely polyimidazoles chain overlaying on the surface of MXene (Ti3C2@IMIZ), was prepared by a simple method.
34323712	1	31	theme	polyimidazoles	188:201	arg1	chain					203:207	polyimidazoles chain	188:207	polyimidazoles chain overlaying on the surface of MXene (Ti3C2@IMIZ)	188:255	In this work, a novel imidazoles-MXene hybrid composite, namely polyimidazoles chain overlaying on the surface of MXene (Ti3C2@IMIZ), was prepared by a simple method.
34323712	0	32	theme	MXene	29:33	arg1	functionalization					8:24	Surface functionalization	0:24	Surface functionalization of MXene with chitosan through in-situ formation of polyimidazoles and its adsorption properties.	0:122	Surface functionalization of MXene with chitosan through in-situ formation of polyimidazoles and its adsorption properties.
34323712	5	33	theme	operational	879:889	arg1	factors					891:897	operational factors	879:897	operational factors	879:897	Detailed adsorption characteristics of Ti3C2@IMIZ including operational factors, adsorption kinetics and isotherms models were investigated.
34323712	9	34	theme	excellent	1320:1328	arg1	reproducibility					1330:1344	The excellent reproducibility	1316:1344	The excellent reproducibility	1316:1344	The excellent reproducibility suggests that Ti3C2@IMIZ may be a potential candidate for remove of Cr(Ⅵ) in actual sewage treatment.
34323712	3	35	with	imidazoles	522:531	arg1	structure					555:563	an ordered chain structure	538:563	an ordered chain structure	538:563	Based on the characterization results, we demonstrated that a thin layer imidazoles with an ordered chain structure was embedded on the surface of Ti3C2, which resulted in the formation of a novel imidazoles-MXene hybrid composite.
34323712	2	36	theme	in	332:333	arg1	growth					340:345	in situ growth	332:345	in situ growth on the surface of MXenes	332:370	Through this strategy, imidazoles can be in situ growth on the surface of MXenes via a facile multicomponent reaction using chitosan as a renewable reactant.
34323712	8	37	theme	electrostatic	1289:1301	arg1	interaction					1303:1313	electrostatic interaction	1289:1313	electrostatic interaction	1289:1313	The adsorption behavior and process analysis show that the adsorption mechanism is mainly physical adsorption through electrostatic interaction.
34323712	3	38	theme	thin	511:514	arg1	embedded					569:576	embedded	569:576	embedded	569:576	Based on the characterization results, we demonstrated that a thin layer imidazoles with an ordered chain structure was embedded on the surface of Ti3C2, which resulted in the formation of a novel imidazoles-MXene hybrid composite.
34323712	3	38	theme	thin	511:514	arg1	imidazoles					522:531	a thin layer imidazoles	509:531	a thin layer imidazoles with an ordered chain structure	509:563	Based on the characterization results, we demonstrated that a thin layer imidazoles with an ordered chain structure was embedded on the surface of Ti3C2, which resulted in the formation of a novel imidazoles-MXene hybrid composite.
34323712	9	39	theme	potential	1380:1388	arg1	candidate					1390:1398	a potential candidate	1378:1398	a potential candidate for remove of Cr(Ⅵ)	1378:1418	The excellent reproducibility suggests that Ti3C2@IMIZ may be a potential candidate for remove of Cr(Ⅵ) in actual sewage treatment.
34323712	9	39	theme	potential	1380:1388	arg1	IMIZ					1366:1369	Ti3C2@IMIZ	1360:1369	Ti3C2@IMIZ	1360:1369	The excellent reproducibility suggests that Ti3C2@IMIZ may be a potential candidate for remove of Cr(Ⅵ) in actual sewage treatment.
34323712	2	40	theme	MXenes	365:370	arg1	surface					354:360	the surface	350:360	the surface of MXenes	350:370	Through this strategy, imidazoles can be in situ growth on the surface of MXenes via a facile multicomponent reaction using chitosan as a renewable reactant.
34323712	8	41	theme	adsorption	1175:1184	arg1	behavior					1186:1193	The adsorption behavior	1171:1193	The adsorption behavior	1171:1193	The adsorption behavior and process analysis show that the adsorption mechanism is mainly physical adsorption through electrostatic interaction.
34323712	7	42	dep	Cr	1108:1109	arg1	VI					1111:1112	VI	1111:1112	VI	1111:1112	The adsorption of Cr(VI) and reduction of Cr(VI) to Cr(III) contribute to elimination of Cr(VI) species.
34323712	3	43	theme	Ti3C2	596:600	arg1	surface					585:591	the surface	581:591	the surface of Ti3C2, which resulted in the formation of a novel imidazoles-MXene hybrid composite	581:678	Based on the characterization results, we demonstrated that a thin layer imidazoles with an ordered chain structure was embedded on the surface of Ti3C2, which resulted in the formation of a novel imidazoles-MXene hybrid composite.
34323712	3	44	theme	ordered	541:547	arg1	structure					555:563	an ordered chain structure	538:563	an ordered chain structure	538:563	Based on the characterization results, we demonstrated that a thin layer imidazoles with an ordered chain structure was embedded on the surface of Ti3C2, which resulted in the formation of a novel imidazoles-MXene hybrid composite.
34323712	2	45	theme	facile	378:383	arg1	reaction					400:407	a facile multicomponent reaction	376:407	a facile multicomponent reaction using chitosan as a renewable reactant	376:446	Through this strategy, imidazoles can be in situ growth on the surface of MXenes via a facile multicomponent reaction using chitosan as a renewable reactant.
34323712	2	46	from	growth	340:345	arg1	surface					354:360	the surface	350:360	the surface of MXenes	350:370	Through this strategy, imidazoles can be in situ growth on the surface of MXenes via a facile multicomponent reaction using chitosan as a renewable reactant.
34323712	0	47	theme	in-situ	57:63	arg1	formation					65:73	in-situ formation	57:73	in-situ formation of polyimidazoles	57:91	Surface functionalization of MXene with chitosan through in-situ formation of polyimidazoles and its adsorption properties.
34323712	1	48	theme	simple	276:281	arg1	method					283:288	a simple method	274:288	a simple method	274:288	In this work, a novel imidazoles-MXene hybrid composite, namely polyimidazoles chain overlaying on the surface of MXene (Ti3C2@IMIZ), was prepared by a simple method.
34323712	8	49	theme	process	1199:1205	arg1	analysis					1207:1214	process analysis	1199:1214	process analysis	1199:1214	The adsorption behavior and process analysis show that the adsorption mechanism is mainly physical adsorption through electrostatic interaction.
34323712	4	50	theme	heavy	779:783	arg1	ions					791:794	heavy metal ions	779:794	heavy metal ions of Cr(Ⅵ)	779:803	The adsorption performance of Ti3C2@IMIZ for removal environmental pollutants was evaluated using heavy metal ions of Cr(Ⅵ) as adsorbate.
34323712	6	51	theme	low	1022:1024	arg1	toxicity					1026:1033	low toxicity	1022:1033	low toxicity	1022:1033	XPS analysis showed that Cr(VI) was converted to Cr(III) with low toxicity during the adsorption process.
34323712	8	52	theme	physical	1261:1268	arg1	mechanism					1241:1249	the adsorption mechanism	1226:1249	the adsorption mechanism	1226:1249	The adsorption behavior and process analysis show that the adsorption mechanism is mainly physical adsorption through electrostatic interaction.
34323712	8	52	theme	physical	1261:1268	arg1	adsorption					1270:1279	mainly physical adsorption	1254:1279	mainly physical adsorption	1254:1279	The adsorption behavior and process analysis show that the adsorption mechanism is mainly physical adsorption through electrostatic interaction.
34323712	9	53	theme	@	1365:1365	arg1	candidate					1390:1398	a potential candidate	1378:1398	a potential candidate for remove of Cr(Ⅵ)	1378:1418	The excellent reproducibility suggests that Ti3C2@IMIZ may be a potential candidate for remove of Cr(Ⅵ) in actual sewage treatment.
34323712	9	53	theme	@	1365:1365	arg1	IMIZ					1366:1369	Ti3C2@IMIZ	1360:1369	Ti3C2@IMIZ	1360:1369	The excellent reproducibility suggests that Ti3C2@IMIZ may be a potential candidate for remove of Cr(Ⅵ) in actual sewage treatment.
34323712	6	54	dep	converted	996:1004	arg1	VI					988:989	VI	988:989	VI	988:989	XPS analysis showed that Cr(VI) was converted to Cr(III) with low toxicity during the adsorption process.
34323712	8	55	theme	adsorption	1230:1239	arg1	mechanism					1241:1249	the adsorption mechanism	1226:1249	the adsorption mechanism	1226:1249	The adsorption behavior and process analysis show that the adsorption mechanism is mainly physical adsorption through electrostatic interaction.
34323712	8	55	theme	adsorption	1230:1239	arg1	adsorption					1270:1279	mainly physical adsorption	1254:1279	mainly physical adsorption	1254:1279	The adsorption behavior and process analysis show that the adsorption mechanism is mainly physical adsorption through electrostatic interaction.
34323712	0	56	with	functionalization	8:24	arg1	chitosan					40:47	chitosan	40:47	chitosan	40:47	Surface functionalization of MXene with chitosan through in-situ formation of polyimidazoles and its adsorption properties.
34323712	9	57	theme	Ti3C2	1360:1364	arg1	candidate					1390:1398	a potential candidate	1378:1398	a potential candidate for remove of Cr(Ⅵ)	1378:1418	The excellent reproducibility suggests that Ti3C2@IMIZ may be a potential candidate for remove of Cr(Ⅵ) in actual sewage treatment.
34323712	9	57	theme	Ti3C2	1360:1364	arg1	IMIZ					1366:1369	Ti3C2@IMIZ	1360:1369	Ti3C2@IMIZ	1360:1369	The excellent reproducibility suggests that Ti3C2@IMIZ may be a potential candidate for remove of Cr(Ⅵ) in actual sewage treatment.
34323712	7	58	dep	Cr	1155:1156	arg1	VI					1158:1159	VI	1158:1159	VI	1158:1159	The adsorption of Cr(VI) and reduction of Cr(VI) to Cr(III) contribute to elimination of Cr(VI) species.
34323712	7	58	dep	Cr	1155:1156	arg1	species					1162:1168	species	1162:1168	Cr(VI) species	1155:1168	The adsorption of Cr(VI) and reduction of Cr(VI) to Cr(III) contribute to elimination of Cr(VI) species.
34323712	4	59	theme	removal	726:732	arg1	pollutants					748:757	removal environmental pollutants	726:757	removal environmental pollutants	726:757	The adsorption performance of Ti3C2@IMIZ for removal environmental pollutants was evaluated using heavy metal ions of Cr(Ⅵ) as adsorbate.
34323712	3	60	theme	chain	549:553	arg1	structure					555:563	an ordered chain structure	538:563	an ordered chain structure	538:563	Based on the characterization results, we demonstrated that a thin layer imidazoles with an ordered chain structure was embedded on the surface of Ti3C2, which resulted in the formation of a novel imidazoles-MXene hybrid composite.
34323712	5	61	theme	adsorption	828:837	arg1	isotherms					924:932	isotherms	924:932	isotherms	924:932	Detailed adsorption characteristics of Ti3C2@IMIZ including operational factors, adsorption kinetics and isotherms models were investigated.
34323712	5	61	theme	adsorption	828:837	arg1	factors					891:897	operational factors	879:897	operational factors	879:897	Detailed adsorption characteristics of Ti3C2@IMIZ including operational factors, adsorption kinetics and isotherms models were investigated.
34323712	5	61	theme	adsorption	828:837	arg1	kinetics					911:918	adsorption kinetics	900:918	adsorption kinetics	900:918	Detailed adsorption characteristics of Ti3C2@IMIZ including operational factors, adsorption kinetics and isotherms models were investigated.
34323712	5	61	theme	adsorption	828:837	arg1	characteristics					839:853	Detailed adsorption characteristics	819:853	Detailed adsorption characteristics of Ti3C2@IMIZ including operational factors, adsorption kinetics and isotherms models	819:939	Detailed adsorption characteristics of Ti3C2@IMIZ including operational factors, adsorption kinetics and isotherms models were investigated.
34323712	6	62	theme	adsorption	1046:1055	arg1	process					1057:1063	the adsorption process	1042:1063	the adsorption process	1042:1063	XPS analysis showed that Cr(VI) was converted to Cr(III) with low toxicity during the adsorption process.
34323712	4	63	theme	@	716:716	arg1	IMIZ					717:720	Ti3C2@IMIZ	711:720	Ti3C2@IMIZ for removal environmental pollutants	711:757	The adsorption performance of Ti3C2@IMIZ for removal environmental pollutants was evaluated using heavy metal ions of Cr(Ⅵ) as adsorbate.
34323712	4	64	theme	IMIZ	717:720	arg1	performance					696:706	The adsorption performance	681:706	The adsorption performance of Ti3C2@IMIZ for removal environmental pollutants	681:757	The adsorption performance of Ti3C2@IMIZ for removal environmental pollutants was evaluated using heavy metal ions of Cr(Ⅵ) as adsorbate.
34323712	5	65	theme	@	863:863	arg1	IMIZ					864:867	Ti3C2@IMIZ	858:867	Ti3C2@IMIZ	858:867	Detailed adsorption characteristics of Ti3C2@IMIZ including operational factors, adsorption kinetics and isotherms models were investigated.
34323712	5	66	theme	Ti3C2	858:862	arg1	IMIZ					864:867	Ti3C2@IMIZ	858:867	Ti3C2@IMIZ	858:867	Detailed adsorption characteristics of Ti3C2@IMIZ including operational factors, adsorption kinetics and isotherms models were investigated.
34323712	7	67	theme	Cr	1108:1109	arg1	reduction					1095:1103	reduction	1095:1103	reduction of Cr(VI)	1095:1113	The adsorption of Cr(VI) and reduction of Cr(VI) to Cr(III) contribute to elimination of Cr(VI) species.
34323712	7	67	theme	Cr	1108:1109	arg1	Cr					1084:1085	Cr	1084:1085	Cr(VI)	1084:1089	The adsorption of Cr(VI) and reduction of Cr(VI) to Cr(III) contribute to elimination of Cr(VI) species.
34323712	3	68	theme	characterization	462:477	arg1	results					479:485	the characterization results	458:485	the characterization results	458:485	Based on the characterization results, we demonstrated that a thin layer imidazoles with an ordered chain structure was embedded on the surface of Ti3C2, which resulted in the formation of a novel imidazoles-MXene hybrid composite.
34323712	1	69	theme	MXene	238:242	arg1	surface					227:233	the surface	223:233	the surface of MXene (Ti3C2@IMIZ)	223:255	In this work, a novel imidazoles-MXene hybrid composite, namely polyimidazoles chain overlaying on the surface of MXene (Ti3C2@IMIZ), was prepared by a simple method.
34323712	4	70	theme	environmental	734:746	arg1	pollutants					748:757	removal environmental pollutants	726:757	removal environmental pollutants	726:757	The adsorption performance of Ti3C2@IMIZ for removal environmental pollutants was evaluated using heavy metal ions of Cr(Ⅵ) as adsorbate.
34323712	1	71	theme	novel	140:144	arg1	composite					170:178	a novel imidazoles-MXene hybrid composite	138:178	a novel imidazoles-MXene hybrid composite	138:178	In this work, a novel imidazoles-MXene hybrid composite, namely polyimidazoles chain overlaying on the surface of MXene (Ti3C2@IMIZ), was prepared by a simple method.
34323712	1	71	theme	novel	140:144	arg1	chain					203:207	polyimidazoles chain	188:207	polyimidazoles chain overlaying on the surface of MXene (Ti3C2@IMIZ)	188:255	In this work, a novel imidazoles-MXene hybrid composite, namely polyimidazoles chain overlaying on the surface of MXene (Ti3C2@IMIZ), was prepared by a simple method.
34323712	7	72	theme	Cr	1084:1085	arg1	adsorption					1070:1079	The adsorption	1066:1079	The adsorption of Cr(VI) and reduction of Cr(VI) to Cr(III)	1066:1124	The adsorption of Cr(VI) and reduction of Cr(VI) to Cr(III) contribute to elimination of Cr(VI) species.
32673719	8	0	theme	CNC-based	1485:1493	arg1	composite					1495:1503	CNC-based composite	1485:1503	CNC-based composite	1485:1503	The H-lignin provides a new insight into the multifunctional exploration of CNC-based composite.
32673719	2	1	theme	hydrogen	368:375	arg1	strategy					395:402	a hydrogen bonding tailoring strategy	366:402	a hydrogen bonding tailoring strategy that can produce CNC/lignin films with UV blocking and antioxidant activity, while bypassing the loss of mechanical strength	366:527	In this work, we demonstrated a hydrogen bonding tailoring strategy that can produce CNC/lignin films with UV blocking and antioxidant activity, while bypassing the loss of mechanical strength.
32673719	7	2	theme	crystallinity	1385:1397	arg1	property					1399:1406	crystallinity property	1385:1406	crystallinity property	1385:1406	The CNC-based composites showed better thermal stability and improved crystallinity property.
32673719	1	3	theme	cellulose	277:285	arg1	nanocrystals					287:298	cellulose nanocrystals	277:298	cellulose nanocrystals (CNC)	277:304	It has been a huge challenge to obtain simultaneously excellent mechanical strength and desirable multifunctionality from the cellulose nanocrystals (CNC) based food packing materials.
32673719	1	3	theme	cellulose	277:285	arg1	CNC					301:303	CNC	301:303	CNC	301:303	It has been a huge challenge to obtain simultaneously excellent mechanical strength and desirable multifunctionality from the cellulose nanocrystals (CNC) based food packing materials.
32673719	5	4	dep	structure	994:1002	arg1	The					981:983	The	981:983	The	981:983	The phenolic structure and the hydrogen donation of H-lignin also endowed the resulting CNC composites with excellent UV blocking and antioxidant activity.
32673719	1	5	theme	huge	165:168	arg1	challenge					170:178	a huge challenge	163:178	a huge challenge to obtain simultaneously excellent mechanical strength and desirable multifunctionality from the cellulose nanocrystals (CNC) based food packing materials	163:333	It has been a huge challenge to obtain simultaneously excellent mechanical strength and desirable multifunctionality from the cellulose nanocrystals (CNC) based food packing materials.
32673719	1	5	theme	huge	165:168	arg1	It					151:152	It	151:152	It	151:152	It has been a huge challenge to obtain simultaneously excellent mechanical strength and desirable multifunctionality from the cellulose nanocrystals (CNC) based food packing materials.
32673719	9	6	theme	new	1524:1526	arg1	avenue					1528:1533	a new avenue	1522:1533	a new avenue for the next generation's biodegradable food packing materials from cellulose-sourced composites	1522:1630	This work opens a new avenue for the next generation's biodegradable food packing materials from cellulose-sourced composites.
32673719	6	7	theme	experimental	1141:1152	arg1	results					1154:1160	The experimental results	1137:1160	The experimental results	1137:1160	The experimental results indicated that the H-lignin could bring about 34% and 63% increase in tensile strength and Young's modulus, respectively, higher than the reported ones.
32673719	1	8	dep	based	306:310	arg1	nanocrystals					287:298	cellulose nanocrystals	277:298	cellulose nanocrystals (CNC)	277:304	It has been a huge challenge to obtain simultaneously excellent mechanical strength and desirable multifunctionality from the cellulose nanocrystals (CNC) based food packing materials.
32673719	1	8	dep	based	306:310	arg1	CNC					301:303	CNC	301:303	CNC	301:303	It has been a huge challenge to obtain simultaneously excellent mechanical strength and desirable multifunctionality from the cellulose nanocrystals (CNC) based food packing materials.
32673719	1	9	from	materials	325:333	arg1	strength					226:233	excellent mechanical strength	205:233	excellent mechanical strength	205:233	It has been a huge challenge to obtain simultaneously excellent mechanical strength and desirable multifunctionality from the cellulose nanocrystals (CNC) based food packing materials.
32673719	1	9	from	materials	325:333	arg1	multifunctionality					249:266	desirable multifunctionality	239:266	desirable multifunctionality	239:266	It has been a huge challenge to obtain simultaneously excellent mechanical strength and desirable multifunctionality from the cellulose nanocrystals (CNC) based food packing materials.
32673719	9	10	theme	cellulose-sourced	1603:1619	arg1	composites					1621:1630	cellulose-sourced composites	1603:1630	cellulose-sourced composites	1603:1630	This work opens a new avenue for the next generation's biodegradable food packing materials from cellulose-sourced composites.
32673719	2	11	with	films	432:436	arg1	activity					471:478	UV blocking and antioxidant activity	443:478	UV blocking and antioxidant activity	443:478	In this work, we demonstrated a hydrogen bonding tailoring strategy that can produce CNC/lignin films with UV blocking and antioxidant activity, while bypassing the loss of mechanical strength.
32673719	6	12	theme	%	1218:1218	arg1	increase					1220:1227	63% increase	1216:1227	63% increase in tensile strength and Young's modulus	1216:1267	The experimental results indicated that the H-lignin could bring about 34% and 63% increase in tensile strength and Young's modulus, respectively, higher than the reported ones.
32673719	0	13	dep	blocking	116:123	arg1	UV					113:114	UV	113:114	UV	113:114	Strong biodegradable cellulose materials with improved crystallinity via hydrogen bonding tailoring strategy for UV blocking and antioxidant activity.
32673719	4	14	theme	CNC	750:752	arg1	matrix					754:759	CNC matrix	750:759	CNC matrix	750:759	By assembling the polyester modified lignin (H-lignin) into CNC matrix, the hydrogen bonding crosslinks between the H-lignin and CNC chains were successfully promoted, resulting in the CNC composites with the significantly improved mechanical strength, UV blocking and antioxidant activity.
32673719	9	15	from	composites	1621:1630	arg1	materials					1588:1596	the next generation's biodegradable food packing materials	1539:1596	the next generation's biodegradable food packing materials from cellulose-sourced composites	1539:1630	This work opens a new avenue for the next generation's biodegradable food packing materials from cellulose-sourced composites.
32673719	4	16	theme	H-lignin	806:813	arg1	chains					823:828	the H-lignin and CNC chains	802:828	chains	823:828	By assembling the polyester modified lignin (H-lignin) into CNC matrix, the hydrogen bonding crosslinks between the H-lignin and CNC chains were successfully promoted, resulting in the CNC composites with the significantly improved mechanical strength, UV blocking and antioxidant activity.
32673719	7	17	theme	better	1347:1352	arg1	stability					1362:1370	better thermal stability	1347:1370	better thermal stability	1347:1370	The CNC-based composites showed better thermal stability and improved crystallinity property.
32673719	0	18	theme	blocking	116:123	arg1	activity					141:148	UV blocking and antioxidant activity	113:148	UV blocking and antioxidant activity	113:148	Strong biodegradable cellulose materials with improved crystallinity via hydrogen bonding tailoring strategy for UV blocking and antioxidant activity.
32673719	2	19	dep	blocking	446:453	arg1	UV					443:444	UV	443:444	UV	443:444	In this work, we demonstrated a hydrogen bonding tailoring strategy that can produce CNC/lignin films with UV blocking and antioxidant activity, while bypassing the loss of mechanical strength.
32673719	2	20	theme	antioxidant	459:469	arg1	activity					471:478	UV blocking and antioxidant activity	443:478	UV blocking and antioxidant activity	443:478	In this work, we demonstrated a hydrogen bonding tailoring strategy that can produce CNC/lignin films with UV blocking and antioxidant activity, while bypassing the loss of mechanical strength.
32673719	6	21	from	increase	1220:1227	arg1	strength					1240:1247	tensile strength	1232:1247	tensile strength	1232:1247	The experimental results indicated that the H-lignin could bring about 34% and 63% increase in tensile strength and Young's modulus, respectively, higher than the reported ones.
32673719	0	22	theme	biodegradable	7:19	arg1	materials					31:39	Strong biodegradable cellulose materials	0:39	Strong biodegradable cellulose materials with improved crystallinity via hydrogen bonding tailoring strategy for UV blocking and antioxidant activity.	0:149	Strong biodegradable cellulose materials with improved crystallinity via hydrogen bonding tailoring strategy for UV blocking and antioxidant activity.
32673719	8	23	theme	multifunctional	1454:1468	arg1	exploration					1470:1480	the multifunctional exploration	1450:1480	the multifunctional exploration of CNC-based composite	1450:1503	The H-lignin provides a new insight into the multifunctional exploration of CNC-based composite.
32673719	4	24	theme	polyester	708:716	arg1	H-lignin					735:742	H-lignin	735:742	H-lignin	735:742	By assembling the polyester modified lignin (H-lignin) into CNC matrix, the hydrogen bonding crosslinks between the H-lignin and CNC chains were successfully promoted, resulting in the CNC composites with the significantly improved mechanical strength, UV blocking and antioxidant activity.
32673719	4	24	theme	polyester	708:716	arg1	lignin					727:732	the polyester modified lignin	704:732	the polyester modified lignin (H-lignin)	704:743	By assembling the polyester modified lignin (H-lignin) into CNC matrix, the hydrogen bonding crosslinks between the H-lignin and CNC chains were successfully promoted, resulting in the CNC composites with the significantly improved mechanical strength, UV blocking and antioxidant activity.
32673719	0	25	theme	Strong	0:5	arg1	materials					31:39	Strong biodegradable cellulose materials	0:39	Strong biodegradable cellulose materials with improved crystallinity via hydrogen bonding tailoring strategy for UV blocking and antioxidant activity.	0:149	Strong biodegradable cellulose materials with improved crystallinity via hydrogen bonding tailoring strategy for UV blocking and antioxidant activity.
32673719	6	26	theme	tensile	1232:1238	arg1	strength					1240:1247	tensile strength	1232:1247	tensile strength	1232:1247	The experimental results indicated that the H-lignin could bring about 34% and 63% increase in tensile strength and Young's modulus, respectively, higher than the reported ones.
32673719	4	27	theme	CNC	875:877	arg1	composites					879:888	the CNC composites	871:888	the CNC composites with the significantly improved mechanical strength, UV blocking and antioxidant activity	871:978	By assembling the polyester modified lignin (H-lignin) into CNC matrix, the hydrogen bonding crosslinks between the H-lignin and CNC chains were successfully promoted, resulting in the CNC composites with the significantly improved mechanical strength, UV blocking and antioxidant activity.
32673719	7	28	theme	CNC-based	1319:1327	arg1	composites					1329:1338	The CNC-based composites	1315:1338	The CNC-based composites	1315:1338	The CNC-based composites showed better thermal stability and improved crystallinity property.
32673719	7	29	theme	thermal	1354:1360	arg1	stability					1362:1370	better thermal stability	1347:1370	better thermal stability	1347:1370	The CNC-based composites showed better thermal stability and improved crystallinity property.
32673719	1	30	theme	based	306:310	arg1	materials					325:333	the cellulose nanocrystals (CNC) based food packing materials	273:333	the cellulose nanocrystals (CNC) based food packing materials	273:333	It has been a huge challenge to obtain simultaneously excellent mechanical strength and desirable multifunctionality from the cellulose nanocrystals (CNC) based food packing materials.
32673719	6	31	theme	Young	1253:1257	arg1	modulus					1261:1267	Young's modulus	1253:1267	Young's modulus	1253:1267	The experimental results indicated that the H-lignin could bring about 34% and 63% increase in tensile strength and Young's modulus, respectively, higher than the reported ones.
32673719	4	32	theme	bonding	775:781	arg1	crosslinks					783:792	the hydrogen bonding crosslinks	762:792	the hydrogen bonding crosslinks between the H-lignin and CNC chains	762:828	By assembling the polyester modified lignin (H-lignin) into CNC matrix, the hydrogen bonding crosslinks between the H-lignin and CNC chains were successfully promoted, resulting in the CNC composites with the significantly improved mechanical strength, UV blocking and antioxidant activity.
32673719	1	33	theme	excellent	205:213	arg1	strength					226:233	excellent mechanical strength	205:233	excellent mechanical strength	205:233	It has been a huge challenge to obtain simultaneously excellent mechanical strength and desirable multifunctionality from the cellulose nanocrystals (CNC) based food packing materials.
32673719	0	34	theme	cellulose	21:29	arg1	materials					31:39	Strong biodegradable cellulose materials	0:39	Strong biodegradable cellulose materials with improved crystallinity via hydrogen bonding tailoring strategy for UV blocking and antioxidant activity.	0:149	Strong biodegradable cellulose materials with improved crystallinity via hydrogen bonding tailoring strategy for UV blocking and antioxidant activity.
32673719	1	35	theme	food	312:315	arg1	materials					325:333	the cellulose nanocrystals (CNC) based food packing materials	273:333	the cellulose nanocrystals (CNC) based food packing materials	273:333	It has been a huge challenge to obtain simultaneously excellent mechanical strength and desirable multifunctionality from the cellulose nanocrystals (CNC) based food packing materials.
32673719	0	36	theme	antioxidant	129:139	arg1	activity					141:148	UV blocking and antioxidant activity	113:148	UV blocking and antioxidant activity	113:148	Strong biodegradable cellulose materials with improved crystallinity via hydrogen bonding tailoring strategy for UV blocking and antioxidant activity.
32673719	8	37	theme	new	1433:1435	arg1	insight					1437:1443	a new insight	1431:1443	a new insight into the multifunctional exploration of CNC-based composite	1431:1503	The H-lignin provides a new insight into the multifunctional exploration of CNC-based composite.
32673719	4	38	theme	hydrogen	766:773	arg1	bonding					775:781	hydrogen bonding	766:781	the hydrogen bonding crosslinks between the H-lignin and CNC chains	762:828	By assembling the polyester modified lignin (H-lignin) into CNC matrix, the hydrogen bonding crosslinks between the H-lignin and CNC chains were successfully promoted, resulting in the CNC composites with the significantly improved mechanical strength, UV blocking and antioxidant activity.
32673719	6	39	from	%	1210:1210	arg1	strength					1240:1247	tensile strength	1232:1247	tensile strength	1232:1247	The experimental results indicated that the H-lignin could bring about 34% and 63% increase in tensile strength and Young's modulus, respectively, higher than the reported ones.
32673719	1	40	theme	mechanical	215:224	arg1	strength					226:233	excellent mechanical strength	205:233	excellent mechanical strength	205:233	It has been a huge challenge to obtain simultaneously excellent mechanical strength and desirable multifunctionality from the cellulose nanocrystals (CNC) based food packing materials.
32673719	0	41	theme	improved	46:53	arg1	crystallinity					55:67	improved crystallinity	46:67	improved crystallinity	46:67	Strong biodegradable cellulose materials with improved crystallinity via hydrogen bonding tailoring strategy for UV blocking and antioxidant activity.
32673719	1	42	theme	packing	317:323	arg1	materials					325:333	the cellulose nanocrystals (CNC) based food packing materials	273:333	the cellulose nanocrystals (CNC) based food packing materials	273:333	It has been a huge challenge to obtain simultaneously excellent mechanical strength and desirable multifunctionality from the cellulose nanocrystals (CNC) based food packing materials.
32673719	5	43	theme	antioxidant	1115:1125	arg1	activity					1127:1134	antioxidant activity	1115:1134	antioxidant activity	1115:1134	The phenolic structure and the hydrogen donation of H-lignin also endowed the resulting CNC composites with excellent UV blocking and antioxidant activity.
32673719	6	44	theme	reported	1300:1307	arg1	ones					1309:1312	the reported ones	1296:1312	the reported ones	1296:1312	The experimental results indicated that the H-lignin could bring about 34% and 63% increase in tensile strength and Young's modulus, respectively, higher than the reported ones.
32673719	9	45	theme	food	1575:1578	arg1	materials					1588:1596	the next generation's biodegradable food packing materials	1539:1596	the next generation's biodegradable food packing materials from cellulose-sourced composites	1539:1630	This work opens a new avenue for the next generation's biodegradable food packing materials from cellulose-sourced composites.
32673719	6	46	theme	higher	1284:1289	arg1	%					1210:1210	34%	1208:1210	34%	1208:1210	The experimental results indicated that the H-lignin could bring about 34% and 63% increase in tensile strength and Young's modulus, respectively, higher than the reported ones.
32673719	4	47	theme	antioxidant	959:969	arg1	activity					971:978	antioxidant activity	959:978	antioxidant activity	959:978	By assembling the polyester modified lignin (H-lignin) into CNC matrix, the hydrogen bonding crosslinks between the H-lignin and CNC chains were successfully promoted, resulting in the CNC composites with the significantly improved mechanical strength, UV blocking and antioxidant activity.
32673719	3	48	theme	hydroxyl	621:628	arg1	groups					630:635	hydroxyl groups	621:635	hydroxyl groups	621:635	Using a hyperbranched polyester, lignin was first functionalized to increase the amount of hydroxyl groups, thereby increasing the intermolecular interactions.
32673719	0	49	with	materials	31:39	arg1	crystallinity					55:67	improved crystallinity	46:67	improved crystallinity	46:67	Strong biodegradable cellulose materials with improved crystallinity via hydrogen bonding tailoring strategy for UV blocking and antioxidant activity.
32673719	2	50	theme	CNC/lignin	421:430	arg1	films					432:436	CNC/lignin films	421:436	CNC/lignin films with UV blocking and antioxidant activity	421:478	In this work, we demonstrated a hydrogen bonding tailoring strategy that can produce CNC/lignin films with UV blocking and antioxidant activity, while bypassing the loss of mechanical strength.
32673719	9	51	theme	biodegradable	1561:1573	arg1	materials					1588:1596	the next generation's biodegradable food packing materials	1539:1596	the next generation's biodegradable food packing materials from cellulose-sourced composites	1539:1630	This work opens a new avenue for the next generation's biodegradable food packing materials from cellulose-sourced composites.
32673719	2	52	theme	mechanical	509:518	arg1	strength					520:527	mechanical strength	509:527	mechanical strength	509:527	In this work, we demonstrated a hydrogen bonding tailoring strategy that can produce CNC/lignin films with UV blocking and antioxidant activity, while bypassing the loss of mechanical strength.
32673719	5	53	theme	CNC	1069:1071	arg1	composites					1073:1082	the resulting CNC composites	1055:1082	the resulting CNC composites	1055:1082	The phenolic structure and the hydrogen donation of H-lignin also endowed the resulting CNC composites with excellent UV blocking and antioxidant activity.
32673719	9	54	theme	packing	1580:1586	arg1	materials					1588:1596	the next generation's biodegradable food packing materials	1539:1596	the next generation's biodegradable food packing materials from cellulose-sourced composites	1539:1630	This work opens a new avenue for the next generation's biodegradable food packing materials from cellulose-sourced composites.
32673719	4	55	theme	mechanical	922:931	arg1	strength					933:940	the significantly improved mechanical strength	895:940	the significantly improved mechanical strength	895:940	By assembling the polyester modified lignin (H-lignin) into CNC matrix, the hydrogen bonding crosslinks between the H-lignin and CNC chains were successfully promoted, resulting in the CNC composites with the significantly improved mechanical strength, UV blocking and antioxidant activity.
32673719	3	56	theme	intermolecular	661:674	arg1	interactions					676:687	the intermolecular interactions	657:687	the intermolecular interactions	657:687	Using a hyperbranched polyester, lignin was first functionalized to increase the amount of hydroxyl groups, thereby increasing the intermolecular interactions.
32673719	5	57	theme	excellent	1089:1097	arg1	blocking					1102:1109	excellent UV blocking	1089:1109	excellent UV blocking	1089:1109	The phenolic structure and the hydrogen donation of H-lignin also endowed the resulting CNC composites with excellent UV blocking and antioxidant activity.
32673719	4	58	theme	improved	913:920	arg1	strength					933:940	the significantly improved mechanical strength	895:940	the significantly improved mechanical strength	895:940	By assembling the polyester modified lignin (H-lignin) into CNC matrix, the hydrogen bonding crosslinks between the H-lignin and CNC chains were successfully promoted, resulting in the CNC composites with the significantly improved mechanical strength, UV blocking and antioxidant activity.
32673719	8	59	theme	composite	1495:1503	arg1	exploration					1470:1480	the multifunctional exploration	1450:1480	the multifunctional exploration of CNC-based composite	1450:1503	The H-lignin provides a new insight into the multifunctional exploration of CNC-based composite.
32673719	5	60	with	endowed	1047:1053	arg1	blocking					1102:1109	excellent UV blocking	1089:1109	excellent UV blocking	1089:1109	The phenolic structure and the hydrogen donation of H-lignin also endowed the resulting CNC composites with excellent UV blocking and antioxidant activity.
32673719	5	60	with	endowed	1047:1053	arg1	activity					1127:1134	antioxidant activity	1115:1134	antioxidant activity	1115:1134	The phenolic structure and the hydrogen donation of H-lignin also endowed the resulting CNC composites with excellent UV blocking and antioxidant activity.
32673719	4	61	theme	UV	943:944	arg1	blocking					946:953	UV blocking	943:953	UV blocking	943:953	By assembling the polyester modified lignin (H-lignin) into CNC matrix, the hydrogen bonding crosslinks between the H-lignin and CNC chains were successfully promoted, resulting in the CNC composites with the significantly improved mechanical strength, UV blocking and antioxidant activity.
32673719	5	62	theme	resulting	1059:1067	arg1	composites					1073:1082	the resulting CNC composites	1055:1082	the resulting CNC composites	1055:1082	The phenolic structure and the hydrogen donation of H-lignin also endowed the resulting CNC composites with excellent UV blocking and antioxidant activity.
32673719	4	63	theme	CNC	819:821	arg1	chains					823:828	the H-lignin and CNC chains	802:828	chains	823:828	By assembling the polyester modified lignin (H-lignin) into CNC matrix, the hydrogen bonding crosslinks between the H-lignin and CNC chains were successfully promoted, resulting in the CNC composites with the significantly improved mechanical strength, UV blocking and antioxidant activity.
32673719	4	64	theme	modified	718:725	arg1	H-lignin					735:742	H-lignin	735:742	H-lignin	735:742	By assembling the polyester modified lignin (H-lignin) into CNC matrix, the hydrogen bonding crosslinks between the H-lignin and CNC chains were successfully promoted, resulting in the CNC composites with the significantly improved mechanical strength, UV blocking and antioxidant activity.
32673719	4	64	theme	modified	718:725	arg1	lignin					727:732	the polyester modified lignin	704:732	the polyester modified lignin (H-lignin)	704:743	By assembling the polyester modified lignin (H-lignin) into CNC matrix, the hydrogen bonding crosslinks between the H-lignin and CNC chains were successfully promoted, resulting in the CNC composites with the significantly improved mechanical strength, UV blocking and antioxidant activity.
32673719	9	65	theme	next	1543:1546	arg1	generation					1548:1557	the next generation	1539:1557	the next generation's biodegradable food packing materials from cellulose-sourced composites	1539:1630	This work opens a new avenue for the next generation's biodegradable food packing materials from cellulose-sourced composites.
32673719	5	66	theme	phenolic	985:992	arg1	structure					994:1002	phenolic structure	985:1002	phenolic structure	985:1002	The phenolic structure and the hydrogen donation of H-lignin also endowed the resulting CNC composites with excellent UV blocking and antioxidant activity.
32673719	1	67	theme	desirable	239:247	arg1	multifunctionality					249:266	desirable multifunctionality	239:266	desirable multifunctionality	239:266	It has been a huge challenge to obtain simultaneously excellent mechanical strength and desirable multifunctionality from the cellulose nanocrystals (CNC) based food packing materials.
32673719	5	68	theme	UV	1099:1100	arg1	blocking					1102:1109	excellent UV blocking	1089:1109	excellent UV blocking	1089:1109	The phenolic structure and the hydrogen donation of H-lignin also endowed the resulting CNC composites with excellent UV blocking and antioxidant activity.
32673719	5	69	theme	H-lignin	1033:1040	arg1	structure					994:1002	phenolic structure	985:1002	phenolic structure	985:1002	The phenolic structure and the hydrogen donation of H-lignin also endowed the resulting CNC composites with excellent UV blocking and antioxidant activity.
32673719	5	69	theme	H-lignin	1033:1040	arg1	donation					1021:1028	the hydrogen donation	1008:1028	the hydrogen donation	1008:1028	The phenolic structure and the hydrogen donation of H-lignin also endowed the resulting CNC composites with excellent UV blocking and antioxidant activity.
32673719	3	70	theme	hyperbranched	538:550	arg1	polyester					552:560	a hyperbranched polyester	536:560	a hyperbranched polyester	536:560	Using a hyperbranched polyester, lignin was first functionalized to increase the amount of hydroxyl groups, thereby increasing the intermolecular interactions.
32673719	2	71	theme	strength	520:527	arg1	loss					501:504	the loss	497:504	the loss of mechanical strength	497:527	In this work, we demonstrated a hydrogen bonding tailoring strategy that can produce CNC/lignin films with UV blocking and antioxidant activity, while bypassing the loss of mechanical strength.
32673719	5	72	theme	hydrogen	1012:1019	arg1	donation					1021:1028	the hydrogen donation	1008:1028	the hydrogen donation	1008:1028	The phenolic structure and the hydrogen donation of H-lignin also endowed the resulting CNC composites with excellent UV blocking and antioxidant activity.
32673719	3	73	theme	groups	630:635	arg1	amount					611:616	the amount	607:616	the amount of hydroxyl groups	607:635	Using a hyperbranched polyester, lignin was first functionalized to increase the amount of hydroxyl groups, thereby increasing the intermolecular interactions.
32673719	3	73	theme	groups	630:635	arg1	groups					630:635	hydroxyl groups	621:635	hydroxyl groups	621:635	Using a hyperbranched polyester, lignin was first functionalized to increase the amount of hydroxyl groups, thereby increasing the intermolecular interactions.
32673719	2	74	theme	tailoring	385:393	arg1	strategy					395:402	a hydrogen bonding tailoring strategy	366:402	a hydrogen bonding tailoring strategy that can produce CNC/lignin films with UV blocking and antioxidant activity, while bypassing the loss of mechanical strength	366:527	In this work, we demonstrated a hydrogen bonding tailoring strategy that can produce CNC/lignin films with UV blocking and antioxidant activity, while bypassing the loss of mechanical strength.
32673719	6	75	theme	63	1216:1217	arg1	%					1218:1218	%	1218:1218	%	1218:1218	The experimental results indicated that the H-lignin could bring about 34% and 63% increase in tensile strength and Young's modulus, respectively, higher than the reported ones.
32673719	0	76	theme	hydrogen	73:80	arg1	bonding					82:88	hydrogen bonding	73:88	hydrogen bonding tailoring strategy for UV blocking and antioxidant activity	73:148	Strong biodegradable cellulose materials with improved crystallinity via hydrogen bonding tailoring strategy for UV blocking and antioxidant activity.
32673719	2	77	theme	blocking	446:453	arg1	activity					471:478	UV blocking and antioxidant activity	443:478	UV blocking and antioxidant activity	443:478	In this work, we demonstrated a hydrogen bonding tailoring strategy that can produce CNC/lignin films with UV blocking and antioxidant activity, while bypassing the loss of mechanical strength.
32673719	2	78	theme	bonding	377:383	arg1	strategy					395:402	a hydrogen bonding tailoring strategy	366:402	a hydrogen bonding tailoring strategy that can produce CNC/lignin films with UV blocking and antioxidant activity, while bypassing the loss of mechanical strength	366:527	In this work, we demonstrated a hydrogen bonding tailoring strategy that can produce CNC/lignin films with UV blocking and antioxidant activity, while bypassing the loss of mechanical strength.
32673719	4	79	with	composites	879:888	arg1	strength					933:940	the significantly improved mechanical strength	895:940	the significantly improved mechanical strength	895:940	By assembling the polyester modified lignin (H-lignin) into CNC matrix, the hydrogen bonding crosslinks between the H-lignin and CNC chains were successfully promoted, resulting in the CNC composites with the significantly improved mechanical strength, UV blocking and antioxidant activity.
32673719	4	79	with	composites	879:888	arg1	blocking					946:953	UV blocking	943:953	UV blocking	943:953	By assembling the polyester modified lignin (H-lignin) into CNC matrix, the hydrogen bonding crosslinks between the H-lignin and CNC chains were successfully promoted, resulting in the CNC composites with the significantly improved mechanical strength, UV blocking and antioxidant activity.
32673719	4	79	with	composites	879:888	arg1	activity					971:978	antioxidant activity	959:978	antioxidant activity	959:978	By assembling the polyester modified lignin (H-lignin) into CNC matrix, the hydrogen bonding crosslinks between the H-lignin and CNC chains were successfully promoted, resulting in the CNC composites with the significantly improved mechanical strength, UV blocking and antioxidant activity.
32393029	0	0	theme	Thermal	73:79	arg1	Properties					81:90	Mechanical, Morphological, and Thermal Properties	42:90	Mechanical, Morphological, and Thermal Properties of Cellulose/Antarctic Krill Protein Composite Fibers	42:144	Effect of Coagulation Bath Temperature on Mechanical, Morphological, and Thermal Properties of Cellulose/Antarctic Krill Protein Composite Fibers.
32393029	5	1	theme	surface	959:965	arg1	smoother					981:988	smoother	981:988	smoother	981:988	At this temperature, the crystallinity of the composite fibers is improved, the thermal stability is slightly improved, and the surface morphology is smoother.
32393029	5	1	theme	surface	959:965	arg1	morphology					967:976	the surface morphology	955:976	the surface morphology	955:976	At this temperature, the crystallinity of the composite fibers is improved, the thermal stability is slightly improved, and the surface morphology is smoother.
32393029	4	2	from	temperature	818:828	arg1	fibers					803:808	the composite fibers	789:808	the composite fibers at room temperature	789:828	When the coagulation bath temperature is 15 °C, the breaking strength of the composite fibers is 1.64cN/dtex, which is 12% higher than that of the composite fibers at room temperature.
32393029	3	3	theme	C/AKP	590:594	arg1	fibers					606:611	the C/AKP composite fibers	586:611	the C/AKP composite fibers	586:611	The results showed that, when the temperature of the coagulation bath increased from 5 to 25 °C, the intermolecular hydrogen bond content of the C/AKP composite fibers increased from 28.20% to 31.33%.
32393029	0	4	from	Effect	0:5	arg1	Properties					81:90	Mechanical, Morphological, and Thermal Properties	42:90	Mechanical, Morphological, and Thermal Properties of Cellulose/Antarctic Krill Protein Composite Fibers	42:144	Effect of Coagulation Bath Temperature on Mechanical, Morphological, and Thermal Properties of Cellulose/Antarctic Krill Protein Composite Fibers.
32393029	5	5	theme	fibers	887:892	arg1	crystallinity					856:868	the crystallinity	852:868	the crystallinity of the composite fibers	852:892	At this temperature, the crystallinity of the composite fibers is improved, the thermal stability is slightly improved, and the surface morphology is smoother.
32393029	4	6	theme	coagulation	655:665	arg1	°C					690:691	15 °C	687:691	15 °C	687:691	When the coagulation bath temperature is 15 °C, the breaking strength of the composite fibers is 1.64cN/dtex, which is 12% higher than that of the composite fibers at room temperature.
32393029	4	6	theme	coagulation	655:665	arg1	temperature					672:682	the coagulation bath temperature	651:682	the coagulation bath temperature	651:682	When the coagulation bath temperature is 15 °C, the breaking strength of the composite fibers is 1.64cN/dtex, which is 12% higher than that of the composite fibers at room temperature.
32393029	1	7	theme	C/AKP	239:243	arg1	fibers					255:260	C/AKP composite fibers	239:260	C/AKP composite fibers	239:260	Cellulose (C) and Antarctic krill protein (AKP) were dissolved at low temperature, and then C/AKP composite fibers were prepared by wet spinning.
32393029	0	8	theme	Krill	115:119	arg1	Fibers					139:144	Cellulose/Antarctic Krill Protein Composite Fibers	95:144	Cellulose/Antarctic Krill Protein Composite Fibers	95:144	Effect of Coagulation Bath Temperature on Mechanical, Morphological, and Thermal Properties of Cellulose/Antarctic Krill Protein Composite Fibers.
32393029	4	9	theme	composite	723:731	arg1	fibers					733:738	the composite fibers	719:738	the composite fibers	719:738	When the coagulation bath temperature is 15 °C, the breaking strength of the composite fibers is 1.64cN/dtex, which is 12% higher than that of the composite fibers at room temperature.
32393029	2	10	theme	temperature	339:349	arg1	effect					312:317	the effect	308:317	the effect of coagulation bath temperature on the properties of C/AKP composite fibers	308:393	In this paper, the effect of coagulation bath temperature on the properties of C/AKP composite fibers were studied by FT-IR, XRD, DSC, and other tests.
32393029	0	11	theme	Cellulose/Antarctic	95:113	arg1	Fibers					139:144	Cellulose/Antarctic Krill Protein Composite Fibers	95:144	Cellulose/Antarctic Krill Protein Composite Fibers	95:144	Effect of Coagulation Bath Temperature on Mechanical, Morphological, and Thermal Properties of Cellulose/Antarctic Krill Protein Composite Fibers.
32393029	2	12	from	effect	312:317	arg1	properties					358:367	the properties	354:367	the properties of C/AKP composite fibers	354:393	In this paper, the effect of coagulation bath temperature on the properties of C/AKP composite fibers were studied by FT-IR, XRD, DSC, and other tests.
32393029	4	13	theme	bath	667:670	arg1	°C					690:691	15 °C	687:691	15 °C	687:691	When the coagulation bath temperature is 15 °C, the breaking strength of the composite fibers is 1.64cN/dtex, which is 12% higher than that of the composite fibers at room temperature.
32393029	4	13	theme	bath	667:670	arg1	temperature					672:682	the coagulation bath temperature	651:682	the coagulation bath temperature	651:682	When the coagulation bath temperature is 15 °C, the breaking strength of the composite fibers is 1.64cN/dtex, which is 12% higher than that of the composite fibers at room temperature.
32393029	1	14	theme	Antarctic	165:173	arg1	AKP					190:192	AKP	190:192	AKP	190:192	Cellulose (C) and Antarctic krill protein (AKP) were dissolved at low temperature, and then C/AKP composite fibers were prepared by wet spinning.
32393029	1	14	theme	Antarctic	165:173	arg1	protein					181:187	Antarctic krill protein	165:187	Antarctic krill protein (AKP)	165:193	Cellulose (C) and Antarctic krill protein (AKP) were dissolved at low temperature, and then C/AKP composite fibers were prepared by wet spinning.
32393029	3	15	theme	28.20	628:632	arg1	%					633:633	%	633:633	%	633:633	The results showed that, when the temperature of the coagulation bath increased from 5 to 25 °C, the intermolecular hydrogen bond content of the C/AKP composite fibers increased from 28.20% to 31.33%.
32393029	6	16	theme	fibers	1085:1090	arg1	milder					1095:1100	milder	1095:1100	milder	1095:1100	Inspiringly, when zinc sulfate is added to the coagulation bath, the formation process of the fibers is milder.
32393029	6	16	theme	fibers	1085:1090	arg1	process					1070:1076	the formation process	1056:1076	the formation process of the fibers	1056:1090	Inspiringly, when zinc sulfate is added to the coagulation bath, the formation process of the fibers is milder.
32393029	6	17	theme	zinc	1009:1012	arg1	sulfate					1014:1020	zinc sulfate	1009:1020	zinc sulfate	1009:1020	Inspiringly, when zinc sulfate is added to the coagulation bath, the formation process of the fibers is milder.
32393029	1	18	theme	krill	175:179	arg1	AKP					190:192	AKP	190:192	AKP	190:192	Cellulose (C) and Antarctic krill protein (AKP) were dissolved at low temperature, and then C/AKP composite fibers were prepared by wet spinning.
32393029	1	18	theme	krill	175:179	arg1	protein					181:187	Antarctic krill protein	165:187	Antarctic krill protein (AKP)	165:193	Cellulose (C) and Antarctic krill protein (AKP) were dissolved at low temperature, and then C/AKP composite fibers were prepared by wet spinning.
32393029	1	19	theme	composite	245:253	arg1	fibers					255:260	C/AKP composite fibers	239:260	C/AKP composite fibers	239:260	Cellulose (C) and Antarctic krill protein (AKP) were dissolved at low temperature, and then C/AKP composite fibers were prepared by wet spinning.
32393029	4	20	theme	fibers	733:738	arg1	strength					707:714	the breaking strength	694:714	the breaking strength of the composite fibers	694:738	When the coagulation bath temperature is 15 °C, the breaking strength of the composite fibers is 1.64cN/dtex, which is 12% higher than that of the composite fibers at room temperature.
32393029	4	20	theme	fibers	733:738	arg1	1.64cN/dtex					743:753	1.64cN/dtex	743:753	1.64cN/dtex	743:753	When the coagulation bath temperature is 15 °C, the breaking strength of the composite fibers is 1.64cN/dtex, which is 12% higher than that of the composite fibers at room temperature.
32393029	7	21	theme	C/AKP	1117:1121	arg1	fibers					1133:1138	the C/AKP composite fibers	1113:1138	the C/AKP composite fibers	1113:1138	Moreover, the C/AKP composite fibers have excellent antibacterial activity against Escherichia coli and Staphylococcus aureus.
32393029	2	22	theme	bath	334:337	arg1	temperature					339:349	coagulation bath temperature	322:349	coagulation bath temperature	322:349	In this paper, the effect of coagulation bath temperature on the properties of C/AKP composite fibers were studied by FT-IR, XRD, DSC, and other tests.
32393029	0	23	theme	Bath	22:25	arg1	Temperature					27:37	Coagulation Bath Temperature	10:37	Coagulation Bath Temperature	10:37	Effect of Coagulation Bath Temperature on Mechanical, Morphological, and Thermal Properties of Cellulose/Antarctic Krill Protein Composite Fibers.
32393029	6	24	theme	coagulation	1038:1048	arg1	bath					1050:1053	the coagulation bath	1034:1053	the coagulation bath	1034:1053	Inspiringly, when zinc sulfate is added to the coagulation bath, the formation process of the fibers is milder.
32393029	0	25	theme	Composite	129:137	arg1	Fibers					139:144	Cellulose/Antarctic Krill Protein Composite Fibers	95:144	Cellulose/Antarctic Krill Protein Composite Fibers	95:144	Effect of Coagulation Bath Temperature on Mechanical, Morphological, and Thermal Properties of Cellulose/Antarctic Krill Protein Composite Fibers.
32393029	2	26	theme	composite	378:386	arg1	fibers					388:393	C/AKP composite fibers	372:393	C/AKP composite fibers	372:393	In this paper, the effect of coagulation bath temperature on the properties of C/AKP composite fibers were studied by FT-IR, XRD, DSC, and other tests.
32393029	0	27	theme	Coagulation	10:20	arg1	Temperature					27:37	Coagulation Bath Temperature	10:37	Coagulation Bath Temperature	10:37	Effect of Coagulation Bath Temperature on Mechanical, Morphological, and Thermal Properties of Cellulose/Antarctic Krill Protein Composite Fibers.
32393029	3	28	theme	31.33	638:642	arg1	%					633:633	%	633:633	%	633:633	The results showed that, when the temperature of the coagulation bath increased from 5 to 25 °C, the intermolecular hydrogen bond content of the C/AKP composite fibers increased from 28.20% to 31.33%.
32393029	0	29	theme	Protein	121:127	arg1	Fibers					139:144	Cellulose/Antarctic Krill Protein Composite Fibers	95:144	Cellulose/Antarctic Krill Protein Composite Fibers	95:144	Effect of Coagulation Bath Temperature on Mechanical, Morphological, and Thermal Properties of Cellulose/Antarctic Krill Protein Composite Fibers.
32393029	3	30	theme	intermolecular	546:559	arg1	bond					570:573	the intermolecular hydrogen bond	542:573	the intermolecular hydrogen bond content of the C/AKP composite fibers	542:611	The results showed that, when the temperature of the coagulation bath increased from 5 to 25 °C, the intermolecular hydrogen bond content of the C/AKP composite fibers increased from 28.20% to 31.33%.
32393029	7	31	theme	excellent	1145:1153	arg1	activity					1169:1176	excellent antibacterial activity	1145:1176	excellent antibacterial activity	1145:1176	Moreover, the C/AKP composite fibers have excellent antibacterial activity against Escherichia coli and Staphylococcus aureus.
32393029	2	32	theme	C/AKP	372:376	arg1	fibers					388:393	C/AKP composite fibers	372:393	C/AKP composite fibers	372:393	In this paper, the effect of coagulation bath temperature on the properties of C/AKP composite fibers were studied by FT-IR, XRD, DSC, and other tests.
32393029	7	33	theme	composite	1123:1131	arg1	fibers					1133:1138	the C/AKP composite fibers	1113:1138	the C/AKP composite fibers	1113:1138	Moreover, the C/AKP composite fibers have excellent antibacterial activity against Escherichia coli and Staphylococcus aureus.
32393029	4	34	theme	composite	793:801	arg1	fibers					803:808	the composite fibers	789:808	the composite fibers at room temperature	789:828	When the coagulation bath temperature is 15 °C, the breaking strength of the composite fibers is 1.64cN/dtex, which is 12% higher than that of the composite fibers at room temperature.
32393029	3	35	theme	hydrogen	561:568	arg1	bond					570:573	the intermolecular hydrogen bond	542:573	the intermolecular hydrogen bond content of the C/AKP composite fibers	542:611	The results showed that, when the temperature of the coagulation bath increased from 5 to 25 °C, the intermolecular hydrogen bond content of the C/AKP composite fibers increased from 28.20% to 31.33%.
32393029	4	36	theme	breaking	698:705	arg1	strength					707:714	the breaking strength	694:714	the breaking strength of the composite fibers	694:738	When the coagulation bath temperature is 15 °C, the breaking strength of the composite fibers is 1.64cN/dtex, which is 12% higher than that of the composite fibers at room temperature.
32393029	4	36	theme	breaking	698:705	arg1	1.64cN/dtex					743:753	1.64cN/dtex	743:753	1.64cN/dtex	743:753	When the coagulation bath temperature is 15 °C, the breaking strength of the composite fibers is 1.64cN/dtex, which is 12% higher than that of the composite fibers at room temperature.
32393029	7	37	theme	antibacterial	1155:1167	arg1	activity					1169:1176	excellent antibacterial activity	1145:1176	excellent antibacterial activity	1145:1176	Moreover, the C/AKP composite fibers have excellent antibacterial activity against Escherichia coli and Staphylococcus aureus.
32393029	3	38	dep	25	535:536	arg1	to					532:533	to	532:533	to	532:533	The results showed that, when the temperature of the coagulation bath increased from 5 to 25 °C, the intermolecular hydrogen bond content of the C/AKP composite fibers increased from 28.20% to 31.33%.
32393029	0	39	theme	Temperature	27:37	arg1	Effect					0:5	Effect	0:5	Effect of Coagulation Bath Temperature on Mechanical, Morphological, and Thermal Properties of Cellulose/Antarctic Krill Protein Composite Fibers.	0:145	Effect of Coagulation Bath Temperature on Mechanical, Morphological, and Thermal Properties of Cellulose/Antarctic Krill Protein Composite Fibers.
32393029	0	40	theme	Fibers	139:144	arg1	Properties					81:90	Mechanical, Morphological, and Thermal Properties	42:90	Mechanical, Morphological, and Thermal Properties of Cellulose/Antarctic Krill Protein Composite Fibers	42:144	Effect of Coagulation Bath Temperature on Mechanical, Morphological, and Thermal Properties of Cellulose/Antarctic Krill Protein Composite Fibers.
32393029	7	41	contain	have	1140:1143	arg1	fibers					1133:1138	the C/AKP composite fibers	1113:1138	the C/AKP composite fibers	1113:1138	Moreover, the C/AKP composite fibers have excellent antibacterial activity against Escherichia coli and Staphylococcus aureus.
32393029	7	41	contain	have	1140:1143	arg2	activity					1169:1176	excellent antibacterial activity	1145:1176	excellent antibacterial activity	1145:1176	Moreover, the C/AKP composite fibers have excellent antibacterial activity against Escherichia coli and Staphylococcus aureus.
32393029	5	42	theme	composite	877:885	arg1	fibers					887:892	the composite fibers	873:892	the composite fibers	873:892	At this temperature, the crystallinity of the composite fibers is improved, the thermal stability is slightly improved, and the surface morphology is smoother.
32393029	4	43	theme	room	813:816	arg1	temperature					818:828	room temperature	813:828	room temperature	813:828	When the coagulation bath temperature is 15 °C, the breaking strength of the composite fibers is 1.64cN/dtex, which is 12% higher than that of the composite fibers at room temperature.
32393029	1	44	theme	wet	279:281	arg1	spinning					283:290	wet spinning	279:290	wet spinning	279:290	Cellulose (C) and Antarctic krill protein (AKP) were dissolved at low temperature, and then C/AKP composite fibers were prepared by wet spinning.
32393029	3	45	theme	coagulation	498:508	arg1	bath					510:513	the coagulation bath	494:513	the coagulation bath	494:513	The results showed that, when the temperature of the coagulation bath increased from 5 to 25 °C, the intermolecular hydrogen bond content of the C/AKP composite fibers increased from 28.20% to 31.33%.
32393029	0	46	theme	Mechanical	42:51	arg1	Properties					81:90	Mechanical, Morphological, and Thermal Properties	42:90	Mechanical, Morphological, and Thermal Properties of Cellulose/Antarctic Krill Protein Composite Fibers	42:144	Effect of Coagulation Bath Temperature on Mechanical, Morphological, and Thermal Properties of Cellulose/Antarctic Krill Protein Composite Fibers.
32393029	3	47	dep	%	633:633	arg1	to					635:636	to	635:636	to	635:636	The results showed that, when the temperature of the coagulation bath increased from 5 to 25 °C, the intermolecular hydrogen bond content of the C/AKP composite fibers increased from 28.20% to 31.33%.
32393029	3	48	theme	bath	510:513	arg1	temperature					479:489	the temperature	475:489	the temperature of the coagulation bath	475:513	The results showed that, when the temperature of the coagulation bath increased from 5 to 25 °C, the intermolecular hydrogen bond content of the C/AKP composite fibers increased from 28.20% to 31.33%.
32393029	3	49	theme	bond	570:573	arg1	content					575:581	the intermolecular hydrogen bond content	542:581	the intermolecular hydrogen bond content of the C/AKP composite fibers	542:611	The results showed that, when the temperature of the coagulation bath increased from 5 to 25 °C, the intermolecular hydrogen bond content of the C/AKP composite fibers increased from 28.20% to 31.33%.
32393029	2	50	theme	other	432:436	arg1	tests					438:442	other tests	432:442	other tests	432:442	In this paper, the effect of coagulation bath temperature on the properties of C/AKP composite fibers were studied by FT-IR, XRD, DSC, and other tests.
32393029	5	51	theme	thermal	911:917	arg1	stability					919:927	the thermal stability	907:927	the thermal stability	907:927	At this temperature, the crystallinity of the composite fibers is improved, the thermal stability is slightly improved, and the surface morphology is smoother.
32393029	3	52	theme	composite	596:604	arg1	fibers					606:611	the C/AKP composite fibers	586:611	the C/AKP composite fibers	586:611	The results showed that, when the temperature of the coagulation bath increased from 5 to 25 °C, the intermolecular hydrogen bond content of the C/AKP composite fibers increased from 28.20% to 31.33%.
32393029	6	53	theme	formation	1060:1068	arg1	milder					1095:1100	milder	1095:1100	milder	1095:1100	Inspiringly, when zinc sulfate is added to the coagulation bath, the formation process of the fibers is milder.
32393029	6	53	theme	formation	1060:1068	arg1	process					1070:1076	the formation process	1056:1076	the formation process of the fibers	1056:1090	Inspiringly, when zinc sulfate is added to the coagulation bath, the formation process of the fibers is milder.
32393029	2	54	theme	coagulation	322:332	arg1	temperature					339:349	coagulation bath temperature	322:349	coagulation bath temperature	322:349	In this paper, the effect of coagulation bath temperature on the properties of C/AKP composite fibers were studied by FT-IR, XRD, DSC, and other tests.
32393029	1	55	theme	low	213:215	arg1	temperature					217:227	low temperature	213:227	low temperature	213:227	Cellulose (C) and Antarctic krill protein (AKP) were dissolved at low temperature, and then C/AKP composite fibers were prepared by wet spinning.
32393029	0	56	theme	Morphological	54:66	arg1	Properties					81:90	Mechanical, Morphological, and Thermal Properties	42:90	Mechanical, Morphological, and Thermal Properties of Cellulose/Antarctic Krill Protein Composite Fibers	42:144	Effect of Coagulation Bath Temperature on Mechanical, Morphological, and Thermal Properties of Cellulose/Antarctic Krill Protein Composite Fibers.
32393029	3	57	theme	fibers	606:611	arg1	content					575:581	the intermolecular hydrogen bond content	542:581	the intermolecular hydrogen bond content of the C/AKP composite fibers	542:611	The results showed that, when the temperature of the coagulation bath increased from 5 to 25 °C, the intermolecular hydrogen bond content of the C/AKP composite fibers increased from 28.20% to 31.33%.
32393029	2	58	theme	fibers	388:393	arg1	properties					358:367	the properties	354:367	the properties of C/AKP composite fibers	354:393	In this paper, the effect of coagulation bath temperature on the properties of C/AKP composite fibers were studied by FT-IR, XRD, DSC, and other tests.
31917987	0	0	theme	Chitosan/Tigecycline	79:98	arg1	composite					100:108	novel Chitosan/Tigecycline composite	73:108	novel Chitosan/Tigecycline composite	73:108	Synthesis, characterization, and evaluation of antimicrobial activity of novel Chitosan/Tigecycline composite.
31917987	6	1	theme	CS	963:964	arg1	interaction					944:954	the interaction	940:954	the interaction of the CS	940:964	UV-vis spectra confirmed the interaction of the CS and the drug TIGE.
31917987	6	1	theme	CS	963:964	arg1	TIGE					979:982	the drug TIGE	970:982	the drug TIGE	970:982	UV-vis spectra confirmed the interaction of the CS and the drug TIGE.
31917987	7	2	theme	TIGE	1100:1103	arg1	ratios					1090:1095	the ratios	1086:1095	the ratios of TIGE	1086:1103	FE-SEM photos confirm the increasing of TIGE particles on the surface of CS/TIGE films by increasing the ratios of TIGE.
31917987	1	3	from	rate	189:192	arg1	available					164:172	available	164:172	available	164:172	With the increase of antibiotic resistance currently available at an alarming rate, new improved films of Chitosan (CS) have been achieved with different levels of Tigecycline (TIGE) using traditional casting technology.
31917987	5	4	theme	TIGE	897:900	arg1	ratios					887:892	the ratios	883:892	the ratios of TIGE in Chitosan	883:912	FT-IR reveals conservation of the distinctive bands with change in both intensity and position that attributed to increase the ratios of TIGE in Chitosan.
31917987	9	5	theme	obtained	1394:1401	arg1	results					1403:1409	The obtained results	1390:1409	The obtained results	1390:1409	The obtained results indicated that the composite Cs/TIGE can be used in many antimicrobial applications because of its greatly antimicrobial activity.
31917987	0	6	theme	novel	73:77	arg1	composite					100:108	novel Chitosan/Tigecycline composite	73:108	novel Chitosan/Tigecycline composite	73:108	Synthesis, characterization, and evaluation of antimicrobial activity of novel Chitosan/Tigecycline composite.
31917987	2	7	theme	structural	336:345	arg1	characterizations					375:391	The structural, optical, and morphological characterizations	332:391	The structural, optical, and morphological characterizations of the prepared (CS/TIGE) composites	332:428	The structural, optical, and morphological characterizations of the prepared (CS/TIGE) composites were investigated using XRD, FT-IR, UV-visible, and FE-SEM.
31917987	8	8	with	effect	1124:1129	arg1	TIGE					1186:1189	TIGE	1186:1189	TIGE	1186:1189	The antimicrobial effect of Chitosan and five different ratios of Chitosan with TIGE have been identified against four different bacterial species, two gram-negative (Escherichia coli, Bacillus subtilis) and two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa).
31917987	8	9	theme	gram-positive	1318:1330	arg1	bacteria					1332:1339	two gram-positive bacteria	1314:1339	two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa)	1314:1387	The antimicrobial effect of Chitosan and five different ratios of Chitosan with TIGE have been identified against four different bacterial species, two gram-negative (Escherichia coli, Bacillus subtilis) and two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa).
31917987	8	9	theme	gram-positive	1318:1330	arg1	species					1245:1251	four different bacterial species	1220:1251	four different bacterial species	1220:1251	The antimicrobial effect of Chitosan and five different ratios of Chitosan with TIGE have been identified against four different bacterial species, two gram-negative (Escherichia coli, Bacillus subtilis) and two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa).
31917987	0	10	theme	composite	100:108	arg1	activity					61:68	antimicrobial activity	47:68	antimicrobial activity of novel Chitosan/Tigecycline composite	47:108	Synthesis, characterization, and evaluation of antimicrobial activity of novel Chitosan/Tigecycline composite.
31917987	6	11	theme	drug	974:977	arg1	TIGE					979:982	the drug TIGE	970:982	the drug TIGE	970:982	UV-vis spectra confirmed the interaction of the CS and the drug TIGE.
31917987	5	12	from	ratios	887:892	arg1	Chitosan					905:912	Chitosan	905:912	Chitosan	905:912	FT-IR reveals conservation of the distinctive bands with change in both intensity and position that attributed to increase the ratios of TIGE in Chitosan.
31917987	5	13	with	bands	806:810	arg1	change					817:822	change	817:822	change in both intensity and position	817:853	FT-IR reveals conservation of the distinctive bands with change in both intensity and position that attributed to increase the ratios of TIGE in Chitosan.
31917987	1	14	theme	different	255:263	arg1	levels					265:270	different levels	255:270	different levels of Tigecycline (TIGE)	255:292	With the increase of antibiotic resistance currently available at an alarming rate, new improved films of Chitosan (CS) have been achieved with different levels of Tigecycline (TIGE) using traditional casting technology.
31917987	8	15	theme	different	1152:1160	arg1	ratios					1162:1167	five different ratios	1147:1167	five different ratios of Chitosan with TIGE	1147:1189	The antimicrobial effect of Chitosan and five different ratios of Chitosan with TIGE have been identified against four different bacterial species, two gram-negative (Escherichia coli, Bacillus subtilis) and two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa).
31917987	8	16	theme	bacterial	1235:1243	arg1	bacteria					1332:1339	two gram-positive bacteria	1314:1339	two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa)	1314:1387	The antimicrobial effect of Chitosan and five different ratios of Chitosan with TIGE have been identified against four different bacterial species, two gram-negative (Escherichia coli, Bacillus subtilis) and two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa).
31917987	8	16	theme	bacterial	1235:1243	arg1	species					1245:1251	four different bacterial species	1220:1251	four different bacterial species	1220:1251	The antimicrobial effect of Chitosan and five different ratios of Chitosan with TIGE have been identified against four different bacterial species, two gram-negative (Escherichia coli, Bacillus subtilis) and two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa).
31917987	8	16	theme	bacterial	1235:1243	arg1	gram-negative					1258:1270	gram-negative	1258:1270	gram-negative	1258:1270	The antimicrobial effect of Chitosan and five different ratios of Chitosan with TIGE have been identified against four different bacterial species, two gram-negative (Escherichia coli, Bacillus subtilis) and two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa).
31917987	1	17	theme	alarming	180:187	arg1	rate					189:192	an alarming rate	177:192	an alarming rate	177:192	With the increase of antibiotic resistance currently available at an alarming rate, new improved films of Chitosan (CS) have been achieved with different levels of Tigecycline (TIGE) using traditional casting technology.
31917987	4	18	theme	semi-crystalline	569:584	arg1	peak					586:589	a semi-crystalline peak	567:589	a semi-crystalline peak at 2θ = 21° begins to appear at Cs/TIGE2 concentration and its intensity increase with increasing TIGE concentration which indicate the interaction between TIGE and Cs	567:757	XRD pattern shows a semi-crystalline peak at 2θ = 21° begins to appear at Cs/TIGE2 concentration and its intensity increase with increasing TIGE concentration which indicate the interaction between TIGE and Cs.
31917987	3	19	theme	antibacterial	500:512	arg1	characterization					514:529	the antibacterial characterization	496:529	the antibacterial characterization	496:529	Also, the antibacterial characterization was investigated.
31917987	9	20	used	used	1455:1458	arg2	Cs/TIGE					1440:1446	the composite Cs/TIGE	1426:1446	the composite Cs/TIGE	1426:1446	The obtained results indicated that the composite Cs/TIGE can be used in many antimicrobial applications because of its greatly antimicrobial activity.
31917987	9	21	theme	composite	1430:1438	arg1	Cs/TIGE					1440:1446	the composite Cs/TIGE	1426:1446	the composite Cs/TIGE	1426:1446	The obtained results indicated that the composite Cs/TIGE can be used in many antimicrobial applications because of its greatly antimicrobial activity.
31917987	9	22	theme	antimicrobial	1518:1530	arg1	activity					1532:1539	its greatly antimicrobial activity	1506:1539	its greatly antimicrobial activity	1506:1539	The obtained results indicated that the composite Cs/TIGE can be used in many antimicrobial applications because of its greatly antimicrobial activity.
31917987	5	23	from	change	817:822	arg1	position					846:853	position	846:853	position	846:853	FT-IR reveals conservation of the distinctive bands with change in both intensity and position that attributed to increase the ratios of TIGE in Chitosan.
31917987	5	23	from	change	817:822	arg1	intensity					832:840	intensity	832:840	intensity	832:840	FT-IR reveals conservation of the distinctive bands with change in both intensity and position that attributed to increase the ratios of TIGE in Chitosan.
31917987	7	24	theme	films	1066:1070	arg1	surface					1047:1053	the surface	1043:1053	the surface of CS/TIGE films	1043:1070	FE-SEM photos confirm the increasing of TIGE particles on the surface of CS/TIGE films by increasing the ratios of TIGE.
31917987	8	25	theme	different	1225:1233	arg1	bacteria					1332:1339	two gram-positive bacteria	1314:1339	two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa)	1314:1387	The antimicrobial effect of Chitosan and five different ratios of Chitosan with TIGE have been identified against four different bacterial species, two gram-negative (Escherichia coli, Bacillus subtilis) and two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa).
31917987	8	25	theme	different	1225:1233	arg1	species					1245:1251	four different bacterial species	1220:1251	four different bacterial species	1220:1251	The antimicrobial effect of Chitosan and five different ratios of Chitosan with TIGE have been identified against four different bacterial species, two gram-negative (Escherichia coli, Bacillus subtilis) and two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa).
31917987	8	25	theme	different	1225:1233	arg1	gram-negative					1258:1270	gram-negative	1258:1270	gram-negative	1258:1270	The antimicrobial effect of Chitosan and five different ratios of Chitosan with TIGE have been identified against four different bacterial species, two gram-negative (Escherichia coli, Bacillus subtilis) and two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa).
31917987	2	26	theme	morphological	361:373	arg1	characterizations					375:391	The structural, optical, and morphological characterizations	332:391	The structural, optical, and morphological characterizations of the prepared (CS/TIGE) composites	332:428	The structural, optical, and morphological characterizations of the prepared (CS/TIGE) composites were investigated using XRD, FT-IR, UV-visible, and FE-SEM.
31917987	1	27	theme	new	195:197	arg1	films					208:212	new improved films	195:212	new improved films of Chitosan (CS)	195:229	With the increase of antibiotic resistance currently available at an alarming rate, new improved films of Chitosan (CS) have been achieved with different levels of Tigecycline (TIGE) using traditional casting technology.
31917987	2	28	theme	composites	419:428	arg1	characterizations					375:391	The structural, optical, and morphological characterizations	332:391	The structural, optical, and morphological characterizations of the prepared (CS/TIGE) composites	332:428	The structural, optical, and morphological characterizations of the prepared (CS/TIGE) composites were investigated using XRD, FT-IR, UV-visible, and FE-SEM.
31917987	5	29	theme	distinctive	794:804	arg1	bands					806:810	the distinctive bands	790:810	the distinctive bands with change in both intensity and position	790:853	FT-IR reveals conservation of the distinctive bands with change in both intensity and position that attributed to increase the ratios of TIGE in Chitosan.
31917987	1	30	theme	improved	199:206	arg1	films					208:212	new improved films	195:212	new improved films of Chitosan (CS)	195:229	With the increase of antibiotic resistance currently available at an alarming rate, new improved films of Chitosan (CS) have been achieved with different levels of Tigecycline (TIGE) using traditional casting technology.
31917987	8	31	theme	Chitosan	1172:1179	arg1	effect					1124:1129	The antimicrobial effect	1106:1129	The antimicrobial effect of Chitosan	1106:1141	The antimicrobial effect of Chitosan and five different ratios of Chitosan with TIGE have been identified against four different bacterial species, two gram-negative (Escherichia coli, Bacillus subtilis) and two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa).
31917987	8	31	theme	Chitosan	1172:1179	arg1	ratios					1162:1167	five different ratios	1147:1167	five different ratios of Chitosan with TIGE	1147:1189	The antimicrobial effect of Chitosan and five different ratios of Chitosan with TIGE have been identified against four different bacterial species, two gram-negative (Escherichia coli, Bacillus subtilis) and two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa).
31917987	1	32	theme	Tigecycline	275:285	arg1	levels					265:270	different levels	255:270	different levels of Tigecycline (TIGE)	255:292	With the increase of antibiotic resistance currently available at an alarming rate, new improved films of Chitosan (CS) have been achieved with different levels of Tigecycline (TIGE) using traditional casting technology.
31917987	7	33	theme	CS/TIGE	1058:1064	arg1	films					1066:1070	CS/TIGE films	1058:1070	CS/TIGE films	1058:1070	FE-SEM photos confirm the increasing of TIGE particles on the surface of CS/TIGE films by increasing the ratios of TIGE.
31917987	4	34	theme	TIGE	689:692	arg1	concentration					694:706	TIGE concentration	689:706	TIGE concentration which indicate the interaction between TIGE and Cs	689:757	XRD pattern shows a semi-crystalline peak at 2θ = 21° begins to appear at Cs/TIGE2 concentration and its intensity increase with increasing TIGE concentration which indicate the interaction between TIGE and Cs.
31917987	6	35	theme	UV-vis	915:920	arg1	spectra					922:928	UV-vis spectra	915:928	UV-vis spectra	915:928	UV-vis spectra confirmed the interaction of the CS and the drug TIGE.
31917987	8	36	dep	bacteria	1332:1339	arg1	aureus					1357:1362	Staphylococcus aureus	1342:1362	Staphylococcus aureus	1342:1362	The antimicrobial effect of Chitosan and five different ratios of Chitosan with TIGE have been identified against four different bacterial species, two gram-negative (Escherichia coli, Bacillus subtilis) and two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa).
31917987	8	36	dep	bacteria	1332:1339	arg1	aeruginosa					1377:1386	Pseudomonas aeruginosa	1365:1386	Pseudomonas aeruginosa	1365:1386	The antimicrobial effect of Chitosan and five different ratios of Chitosan with TIGE have been identified against four different bacterial species, two gram-negative (Escherichia coli, Bacillus subtilis) and two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa).
31917987	4	37	theme	XRD	549:551	arg1	pattern					553:559	XRD pattern	549:559	XRD pattern	549:559	XRD pattern shows a semi-crystalline peak at 2θ = 21° begins to appear at Cs/TIGE2 concentration and its intensity increase with increasing TIGE concentration which indicate the interaction between TIGE and Cs.
31917987	4	38	theme	intensity	654:662	arg1	increase					664:671	its intensity increase	650:671	its intensity increase	650:671	XRD pattern shows a semi-crystalline peak at 2θ = 21° begins to appear at Cs/TIGE2 concentration and its intensity increase with increasing TIGE concentration which indicate the interaction between TIGE and Cs.
31917987	2	39	theme	prepared	400:407	arg1	composites					419:428	the prepared (CS/TIGE) composites	396:428	the prepared (CS/TIGE) composites	396:428	The structural, optical, and morphological characterizations of the prepared (CS/TIGE) composites were investigated using XRD, FT-IR, UV-visible, and FE-SEM.
31917987	2	39	theme	prepared	400:407	arg1	CS/TIGE					410:416	CS/TIGE	410:416	CS/TIGE	410:416	The structural, optical, and morphological characterizations of the prepared (CS/TIGE) composites were investigated using XRD, FT-IR, UV-visible, and FE-SEM.
31917987	9	40	theme	many	1463:1466	arg1	applications					1482:1493	many antimicrobial applications	1463:1493	many antimicrobial applications	1463:1493	The obtained results indicated that the composite Cs/TIGE can be used in many antimicrobial applications because of its greatly antimicrobial activity.
31917987	1	41	theme	Chitosan	217:224	arg1	films					208:212	new improved films	195:212	new improved films of Chitosan (CS)	195:229	With the increase of antibiotic resistance currently available at an alarming rate, new improved films of Chitosan (CS) have been achieved with different levels of Tigecycline (TIGE) using traditional casting technology.
31917987	0	42	theme	antimicrobial	47:59	arg1	activity					61:68	antimicrobial activity	47:68	antimicrobial activity of novel Chitosan/Tigecycline composite	47:108	Synthesis, characterization, and evaluation of antimicrobial activity of novel Chitosan/Tigecycline composite.
31917987	9	43	theme	antimicrobial	1468:1480	arg1	applications					1482:1493	many antimicrobial applications	1463:1493	many antimicrobial applications	1463:1493	The obtained results indicated that the composite Cs/TIGE can be used in many antimicrobial applications because of its greatly antimicrobial activity.
31917987	5	44	theme	bands	806:810	arg1	conservation					774:785	conservation	774:785	conservation of the distinctive bands with change in both intensity and position that attributed to increase the ratios of TIGE in Chitosan	774:912	FT-IR reveals conservation of the distinctive bands with change in both intensity and position that attributed to increase the ratios of TIGE in Chitosan.
31917987	1	45	theme	available	164:172	arg1	resistance					143:152	antibiotic resistance	132:152	antibiotic resistance currently available at an alarming rate	132:192	With the increase of antibiotic resistance currently available at an alarming rate, new improved films of Chitosan (CS) have been achieved with different levels of Tigecycline (TIGE) using traditional casting technology.
31917987	1	46	theme	traditional	300:310	arg1	technology					320:329	traditional casting technology	300:329	traditional casting technology	300:329	With the increase of antibiotic resistance currently available at an alarming rate, new improved films of Chitosan (CS) have been achieved with different levels of Tigecycline (TIGE) using traditional casting technology.
31917987	8	47	with	ratios	1162:1167	arg1	TIGE					1186:1189	TIGE	1186:1189	TIGE	1186:1189	The antimicrobial effect of Chitosan and five different ratios of Chitosan with TIGE have been identified against four different bacterial species, two gram-negative (Escherichia coli, Bacillus subtilis) and two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa).
31917987	1	48	theme	antibiotic	132:141	arg1	resistance					143:152	antibiotic resistance	132:152	antibiotic resistance currently available at an alarming rate	132:192	With the increase of antibiotic resistance currently available at an alarming rate, new improved films of Chitosan (CS) have been achieved with different levels of Tigecycline (TIGE) using traditional casting technology.
31917987	7	49	theme	TIGE	1025:1028	arg1	particles					1030:1038	TIGE particles	1025:1038	TIGE particles	1025:1038	FE-SEM photos confirm the increasing of TIGE particles on the surface of CS/TIGE films by increasing the ratios of TIGE.
31917987	8	50	dep	gram-negative	1258:1270	arg1	coli					1285:1288	Escherichia coli	1273:1288	Escherichia coli	1273:1288	The antimicrobial effect of Chitosan and five different ratios of Chitosan with TIGE have been identified against four different bacterial species, two gram-negative (Escherichia coli, Bacillus subtilis) and two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa).
31917987	8	50	dep	gram-negative	1258:1270	arg1	subtilis					1300:1307	Bacillus subtilis	1291:1307	Bacillus subtilis	1291:1307	The antimicrobial effect of Chitosan and five different ratios of Chitosan with TIGE have been identified against four different bacterial species, two gram-negative (Escherichia coli, Bacillus subtilis) and two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa).
31917987	1	51	theme	resistance	143:152	arg1	increase					120:127	the increase	116:127	the increase of antibiotic resistance currently available at an alarming rate	116:192	With the increase of antibiotic resistance currently available at an alarming rate, new improved films of Chitosan (CS) have been achieved with different levels of Tigecycline (TIGE) using traditional casting technology.
31917987	8	52	theme	antimicrobial	1110:1122	arg1	effect					1124:1129	The antimicrobial effect	1106:1129	The antimicrobial effect of Chitosan	1106:1141	The antimicrobial effect of Chitosan and five different ratios of Chitosan with TIGE have been identified against four different bacterial species, two gram-negative (Escherichia coli, Bacillus subtilis) and two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa).
31917987	4	53	theme	Cs/TIGE2	623:630	arg1	concentration					632:644	Cs/TIGE2 concentration	623:644	Cs/TIGE2 concentration	623:644	XRD pattern shows a semi-crystalline peak at 2θ = 21° begins to appear at Cs/TIGE2 concentration and its intensity increase with increasing TIGE concentration which indicate the interaction between TIGE and Cs.
31917987	7	54	theme	FE-SEM	985:990	arg1	photos					992:997	FE-SEM photos	985:997	FE-SEM photos	985:997	FE-SEM photos confirm the increasing of TIGE particles on the surface of CS/TIGE films by increasing the ratios of TIGE.
31917987	0	55	theme	activity	61:68	arg1	characterization					11:26	characterization	11:26	characterization	11:26	Synthesis, characterization, and evaluation of antimicrobial activity of novel Chitosan/Tigecycline composite.
31917987	0	55	theme	activity	61:68	arg1	evaluation					33:42	evaluation	33:42	evaluation	33:42	Synthesis, characterization, and evaluation of antimicrobial activity of novel Chitosan/Tigecycline composite.
31917987	0	55	theme	activity	61:68	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis, characterization, and evaluation of antimicrobial activity of novel Chitosan/Tigecycline composite.
31917987	1	56	theme	casting	312:318	arg1	technology					320:329	traditional casting technology	300:329	traditional casting technology	300:329	With the increase of antibiotic resistance currently available at an alarming rate, new improved films of Chitosan (CS) have been achieved with different levels of Tigecycline (TIGE) using traditional casting technology.
31917987	2	57	theme	optical	348:354	arg1	characterizations					375:391	The structural, optical, and morphological characterizations	332:391	The structural, optical, and morphological characterizations of the prepared (CS/TIGE) composites	332:428	The structural, optical, and morphological characterizations of the prepared (CS/TIGE) composites were investigated using XRD, FT-IR, UV-visible, and FE-SEM.
31917987	8	58	theme	Chitosan	1134:1141	arg1	effect					1124:1129	The antimicrobial effect	1106:1129	The antimicrobial effect of Chitosan	1106:1141	The antimicrobial effect of Chitosan and five different ratios of Chitosan with TIGE have been identified against four different bacterial species, two gram-negative (Escherichia coli, Bacillus subtilis) and two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa).
31917987	8	58	theme	Chitosan	1134:1141	arg1	ratios					1162:1167	five different ratios	1147:1167	five different ratios of Chitosan with TIGE	1147:1189	The antimicrobial effect of Chitosan and five different ratios of Chitosan with TIGE have been identified against four different bacterial species, two gram-negative (Escherichia coli, Bacillus subtilis) and two gram-positive bacteria (Staphylococcus aureus, Pseudomonas aeruginosa).
32182593	0	0	theme	ECM	88:90	arg1	structures					92:101	volumetric cell-containing ECM structures	61:101	volumetric cell-containing ECM structures	61:101	Transparent support media for high resolution 3D printing of volumetric cell-containing ECM structures.
32182593	1	1	theme	engineering	157:167	arg1	field					141:145	the field	137:145	the field of tissue engineering	137:167	3D bioprinting may revolutionize the field of tissue engineering by allowing fabrication of bio-structures with a high degree of complexity, fine architecture and heterogeneous composition.
32182593	1	2	theme	architecture	250:261	arg1	degree					223:228	a high degree	216:228	a high degree of complexity, fine architecture and heterogeneous composition	216:291	3D bioprinting may revolutionize the field of tissue engineering by allowing fabrication of bio-structures with a high degree of complexity, fine architecture and heterogeneous composition.
32182593	0	3	theme	cell-containing	72:86	arg1	structures					92:101	volumetric cell-containing ECM structures	61:101	volumetric cell-containing ECM structures	61:101	Transparent support media for high resolution 3D printing of volumetric cell-containing ECM structures.
32182593	5	4	theme	superb	801:806	arg1	qualities					808:816	superb qualities	801:816	superb qualities that enable printing	801:837	We show that a hybrid material, comprised of calcium-alginate nanoparticles and xanthan gum, presents superb qualities that enable printing at high resolution of down to 10 microns, allowing fabrication of complex constructs and cellular structures.
32182593	3	5	contain	possess	412:418	arg2	properties					436:445	weak mechanical properties	420:445	weak mechanical properties	420:445	As such, they generally possess weak mechanical properties and thus must be supported during fabrication in order to prevent the collapse of large, volumetric multi-layered printouts.
32182593	3	5	contain	possess	412:418	arg1	they					397:400	they	397:400	they	397:400	As such, they generally possess weak mechanical properties and thus must be supported during fabrication in order to prevent the collapse of large, volumetric multi-layered printouts.
32182593	6	6	theme	amenability	1115:1125	arg1	range					1089:1093	a wide range	1082:1093	a wide range of temperatures and amenability	1082:1125	This hybrid also presents an exclusive combination of desirable properties such as biocompatibility, high transparency, stability at a wide range of temperatures and amenability to delicate extraction procedures.
32182593	3	7	theme	multi-layered	547:559	arg1	printouts					561:569	large, volumetric multi-layered printouts	529:569	large, volumetric multi-layered printouts	529:569	As such, they generally possess weak mechanical properties and thus must be supported during fabrication in order to prevent the collapse of large, volumetric multi-layered printouts.
32182593	7	8	theme	promising	1410:1418	arg1	medium					1428:1433	a promising support medium	1408:1433	a promising support medium for 3D printing of tissues and organs	1408:1471	Moreover, as fabrication of large, volumetric biological structures may require hours and even days to accomplish, we have demonstrated that the hybrid medium can support prolonged, precise printing for at least 18 h. All these qualities make it a promising support medium for 3D printing of tissues and organs.
32182593	1	9	theme	heterogeneous	267:279	arg1	composition					281:291	heterogeneous composition	267:291	heterogeneous composition	267:291	3D bioprinting may revolutionize the field of tissue engineering by allowing fabrication of bio-structures with a high degree of complexity, fine architecture and heterogeneous composition.
32182593	0	10	theme	structures	92:101	arg1	printing					49:56	high resolution 3D printing	30:56	high resolution 3D printing of volumetric cell-containing ECM structures	30:101	Transparent support media for high resolution 3D printing of volumetric cell-containing ECM structures.
32182593	7	11	theme	organs	1466:1471	arg1	printing					1442:1449	3D printing	1439:1449	3D printing of tissues and organs	1439:1471	Moreover, as fabrication of large, volumetric biological structures may require hours and even days to accomplish, we have demonstrated that the hybrid medium can support prolonged, precise printing for at least 18 h. All these qualities make it a promising support medium for 3D printing of tissues and organs.
32182593	3	12	theme	printouts	561:569	arg1	collapse					517:524	the collapse	513:524	the collapse of large, volumetric multi-layered printouts	513:569	As such, they generally possess weak mechanical properties and thus must be supported during fabrication in order to prevent the collapse of large, volumetric multi-layered printouts.
32182593	2	13	theme	living	374:379	arg1	cells					381:385	living cells	374:385	living cells	374:385	The printing substances in these processes are mostly based on biomaterials and living cells.
32182593	1	14	theme	composition	281:291	arg1	degree					223:228	a high degree	216:228	a high degree of complexity, fine architecture and heterogeneous composition	216:291	3D bioprinting may revolutionize the field of tissue engineering by allowing fabrication of bio-structures with a high degree of complexity, fine architecture and heterogeneous composition.
32182593	7	15	theme	hybrid	1307:1312	arg1	medium					1314:1319	the hybrid medium	1303:1319	the hybrid medium	1303:1319	Moreover, as fabrication of large, volumetric biological structures may require hours and even days to accomplish, we have demonstrated that the hybrid medium can support prolonged, precise printing for at least 18 h. All these qualities make it a promising support medium for 3D printing of tissues and organs.
32182593	4	16	theme	matrix-based	672:683	arg1	biomaterials					685:696	extracellular matrix-based biomaterials	658:696	extracellular matrix-based biomaterials	658:696	In this work, we characterize a uniquely formulated media used to support printing of extracellular matrix-based biomaterials.
32182593	6	17	theme	properties	1013:1022	arg1	combination					988:998	an exclusive combination	975:998	an exclusive combination of desirable properties such as biocompatibility, high transparency, stability	975:1077	This hybrid also presents an exclusive combination of desirable properties such as biocompatibility, high transparency, stability at a wide range of temperatures and amenability to delicate extraction procedures.
32182593	7	18	theme	tissues	1454:1460	arg1	printing					1442:1449	3D printing	1439:1449	3D printing of tissues and organs	1439:1471	Moreover, as fabrication of large, volumetric biological structures may require hours and even days to accomplish, we have demonstrated that the hybrid medium can support prolonged, precise printing for at least 18 h. All these qualities make it a promising support medium for 3D printing of tissues and organs.
32182593	3	19	theme	weak	420:423	arg1	properties					436:445	weak mechanical properties	420:445	weak mechanical properties	420:445	As such, they generally possess weak mechanical properties and thus must be supported during fabrication in order to prevent the collapse of large, volumetric multi-layered printouts.
32182593	5	20	theme	constructs	913:922	arg1	fabrication					890:900	fabrication	890:900	fabrication of complex constructs and cellular structures	890:946	We show that a hybrid material, comprised of calcium-alginate nanoparticles and xanthan gum, presents superb qualities that enable printing at high resolution of down to 10 microns, allowing fabrication of complex constructs and cellular structures.
32182593	4	21	theme	extracellular	658:670	arg1	biomaterials					685:696	extracellular matrix-based biomaterials	658:696	extracellular matrix-based biomaterials	658:696	In this work, we characterize a uniquely formulated media used to support printing of extracellular matrix-based biomaterials.
32182593	7	22	theme	biological	1208:1217	arg1	structures					1219:1228	large, volumetric biological structures	1190:1228	large, volumetric biological structures	1190:1228	Moreover, as fabrication of large, volumetric biological structures may require hours and even days to accomplish, we have demonstrated that the hybrid medium can support prolonged, precise printing for at least 18 h. All these qualities make it a promising support medium for 3D printing of tissues and organs.
32182593	5	23	theme	xanthan	779:785	arg1	gum					787:789	xanthan gum	779:789	xanthan gum	779:789	We show that a hybrid material, comprised of calcium-alginate nanoparticles and xanthan gum, presents superb qualities that enable printing at high resolution of down to 10 microns, allowing fabrication of complex constructs and cellular structures.
32182593	7	24	theme	support	1420:1426	arg1	medium					1428:1433	a promising support medium	1408:1433	a promising support medium for 3D printing of tissues and organs	1408:1471	Moreover, as fabrication of large, volumetric biological structures may require hours and even days to accomplish, we have demonstrated that the hybrid medium can support prolonged, precise printing for at least 18 h. All these qualities make it a promising support medium for 3D printing of tissues and organs.
32182593	7	25	theme	structures	1219:1228	arg1	fabrication					1175:1185	fabrication	1175:1185	fabrication of large, volumetric biological structures	1175:1228	Moreover, as fabrication of large, volumetric biological structures may require hours and even days to accomplish, we have demonstrated that the hybrid medium can support prolonged, precise printing for at least 18 h. All these qualities make it a promising support medium for 3D printing of tissues and organs.
32182593	6	26	theme	desirable	1003:1011	arg1	properties					1013:1022	desirable properties	1003:1022	desirable properties such as biocompatibility, high transparency, stability	1003:1077	This hybrid also presents an exclusive combination of desirable properties such as biocompatibility, high transparency, stability at a wide range of temperatures and amenability to delicate extraction procedures.
32182593	6	26	theme	desirable	1003:1011	arg1	biocompatibility					1032:1047	biocompatibility	1032:1047	biocompatibility	1032:1047	This hybrid also presents an exclusive combination of desirable properties such as biocompatibility, high transparency, stability at a wide range of temperatures and amenability to delicate extraction procedures.
32182593	7	27	dep	large	1190:1194	arg1	volumetric					1197:1206	volumetric	1197:1206	volumetric	1197:1206	Moreover, as fabrication of large, volumetric biological structures may require hours and even days to accomplish, we have demonstrated that the hybrid medium can support prolonged, precise printing for at least 18 h. All these qualities make it a promising support medium for 3D printing of tissues and organs.
32182593	5	28	theme	microns	872:878	arg1	resolution					847:856	high resolution	842:856	high resolution of down to 10 microns	842:878	We show that a hybrid material, comprised of calcium-alginate nanoparticles and xanthan gum, presents superb qualities that enable printing at high resolution of down to 10 microns, allowing fabrication of complex constructs and cellular structures.
32182593	1	29	theme	bio-structures	196:209	arg1	fabrication					181:191	fabrication	181:191	fabrication of bio-structures	181:209	3D bioprinting may revolutionize the field of tissue engineering by allowing fabrication of bio-structures with a high degree of complexity, fine architecture and heterogeneous composition.
32182593	5	30	theme	high	842:845	arg1	resolution					847:856	high resolution	842:856	high resolution of down to 10 microns	842:878	We show that a hybrid material, comprised of calcium-alginate nanoparticles and xanthan gum, presents superb qualities that enable printing at high resolution of down to 10 microns, allowing fabrication of complex constructs and cellular structures.
32182593	5	31	theme	hybrid	714:719	arg1	material					721:728	a hybrid material	712:728	a hybrid material	712:728	We show that a hybrid material, comprised of calcium-alginate nanoparticles and xanthan gum, presents superb qualities that enable printing at high resolution of down to 10 microns, allowing fabrication of complex constructs and cellular structures.
32182593	1	32	theme	3D	104:105	arg1	bioprinting					107:117	3D bioprinting	104:117	3D bioprinting	104:117	3D bioprinting may revolutionize the field of tissue engineering by allowing fabrication of bio-structures with a high degree of complexity, fine architecture and heterogeneous composition.
32182593	6	33	theme	wide	1084:1087	arg1	range					1089:1093	a wide range	1082:1093	a wide range of temperatures and amenability	1082:1125	This hybrid also presents an exclusive combination of desirable properties such as biocompatibility, high transparency, stability at a wide range of temperatures and amenability to delicate extraction procedures.
32182593	3	34	dep	large	529:533	arg1	volumetric					536:545	volumetric	536:545	volumetric	536:545	As such, they generally possess weak mechanical properties and thus must be supported during fabrication in order to prevent the collapse of large, volumetric multi-layered printouts.
32182593	2	35	theme	printing	298:305	arg1	substances					307:316	The printing substances	294:316	The printing substances in these processes	294:335	The printing substances in these processes are mostly based on biomaterials and living cells.
32182593	6	36	theme	extraction	1139:1148	arg1	procedures					1150:1159	delicate extraction procedures	1130:1159	delicate extraction procedures	1130:1159	This hybrid also presents an exclusive combination of desirable properties such as biocompatibility, high transparency, stability at a wide range of temperatures and amenability to delicate extraction procedures.
32182593	0	37	theme	resolution	35:44	arg1	printing					49:56	high resolution 3D printing	30:56	high resolution 3D printing of volumetric cell-containing ECM structures	30:101	Transparent support media for high resolution 3D printing of volumetric cell-containing ECM structures.
32182593	6	38	theme	exclusive	978:986	arg1	combination					988:998	an exclusive combination	975:998	an exclusive combination of desirable properties such as biocompatibility, high transparency, stability	975:1077	This hybrid also presents an exclusive combination of desirable properties such as biocompatibility, high transparency, stability at a wide range of temperatures and amenability to delicate extraction procedures.
32182593	6	39	attach	presents	966:973	arg2	hybrid					954:959	This hybrid	949:959	This hybrid	949:959	This hybrid also presents an exclusive combination of desirable properties such as biocompatibility, high transparency, stability at a wide range of temperatures and amenability to delicate extraction procedures.
32182593	6	39	attach	presents	966:973	arg1	range					1089:1093	a wide range	1082:1093	a wide range of temperatures and amenability	1082:1125	This hybrid also presents an exclusive combination of desirable properties such as biocompatibility, high transparency, stability at a wide range of temperatures and amenability to delicate extraction procedures.
32182593	4	40	theme	biomaterials	685:696	arg1	printing					646:653	printing	646:653	printing of extracellular matrix-based biomaterials	646:696	In this work, we characterize a uniquely formulated media used to support printing of extracellular matrix-based biomaterials.
32182593	1	41	theme	high	218:221	arg1	degree					223:228	a high degree	216:228	a high degree of complexity, fine architecture and heterogeneous composition	216:291	3D bioprinting may revolutionize the field of tissue engineering by allowing fabrication of bio-structures with a high degree of complexity, fine architecture and heterogeneous composition.
32182593	0	42	theme	high	30:33	arg1	printing					49:56	high resolution 3D printing	30:56	high resolution 3D printing of volumetric cell-containing ECM structures	30:101	Transparent support media for high resolution 3D printing of volumetric cell-containing ECM structures.
32182593	3	43	theme	mechanical	425:434	arg1	properties					436:445	weak mechanical properties	420:445	weak mechanical properties	420:445	As such, they generally possess weak mechanical properties and thus must be supported during fabrication in order to prevent the collapse of large, volumetric multi-layered printouts.
32182593	5	44	theme	calcium-alginate	744:759	arg1	nanoparticles					761:773	calcium-alginate nanoparticles	744:773	calcium-alginate nanoparticles	744:773	We show that a hybrid material, comprised of calcium-alginate nanoparticles and xanthan gum, presents superb qualities that enable printing at high resolution of down to 10 microns, allowing fabrication of complex constructs and cellular structures.
32182593	5	45	theme	cellular	928:935	arg1	structures					937:946	cellular structures	928:946	cellular structures	928:946	We show that a hybrid material, comprised of calcium-alginate nanoparticles and xanthan gum, presents superb qualities that enable printing at high resolution of down to 10 microns, allowing fabrication of complex constructs and cellular structures.
32182593	0	46	theme	3D	46:47	arg1	printing					49:56	high resolution 3D printing	30:56	high resolution 3D printing of volumetric cell-containing ECM structures	30:101	Transparent support media for high resolution 3D printing of volumetric cell-containing ECM structures.
32182593	5	47	attach	presents	792:799	arg2	material					721:728	a hybrid material	712:728	a hybrid material	712:728	We show that a hybrid material, comprised of calcium-alginate nanoparticles and xanthan gum, presents superb qualities that enable printing at high resolution of down to 10 microns, allowing fabrication of complex constructs and cellular structures.
32182593	5	47	attach	presents	792:799	arg1	resolution					847:856	high resolution	842:856	high resolution of down to 10 microns	842:878	We show that a hybrid material, comprised of calcium-alginate nanoparticles and xanthan gum, presents superb qualities that enable printing at high resolution of down to 10 microns, allowing fabrication of complex constructs and cellular structures.
32182593	5	48	theme	structures	937:946	arg1	fabrication					890:900	fabrication	890:900	fabrication of complex constructs and cellular structures	890:946	We show that a hybrid material, comprised of calcium-alginate nanoparticles and xanthan gum, presents superb qualities that enable printing at high resolution of down to 10 microns, allowing fabrication of complex constructs and cellular structures.
32182593	7	49	theme	large	1190:1194	arg1	structures					1219:1228	large, volumetric biological structures	1190:1228	large, volumetric biological structures	1190:1228	Moreover, as fabrication of large, volumetric biological structures may require hours and even days to accomplish, we have demonstrated that the hybrid medium can support prolonged, precise printing for at least 18 h. All these qualities make it a promising support medium for 3D printing of tissues and organs.
32182593	7	50	theme	h.	1377:1378	arg1	qualities					1390:1398	at least 18 h. All these qualities	1365:1398	at least 18 h. All these qualities	1365:1398	Moreover, as fabrication of large, volumetric biological structures may require hours and even days to accomplish, we have demonstrated that the hybrid medium can support prolonged, precise printing for at least 18 h. All these qualities make it a promising support medium for 3D printing of tissues and organs.
32182593	4	51	theme	formulated	613:622	arg1	media					624:628	a uniquely formulated media	602:628	a uniquely formulated media used to support printing of extracellular matrix-based biomaterials	602:696	In this work, we characterize a uniquely formulated media used to support printing of extracellular matrix-based biomaterials.
32182593	7	52	theme	3D	1439:1440	arg1	printing					1442:1449	3D printing	1439:1449	3D printing of tissues and organs	1439:1471	Moreover, as fabrication of large, volumetric biological structures may require hours and even days to accomplish, we have demonstrated that the hybrid medium can support prolonged, precise printing for at least 18 h. All these qualities make it a promising support medium for 3D printing of tissues and organs.
32182593	5	53	dep	10	869:870	arg1	to					866:867	to	866:867	to	866:867	We show that a hybrid material, comprised of calcium-alginate nanoparticles and xanthan gum, presents superb qualities that enable printing at high resolution of down to 10 microns, allowing fabrication of complex constructs and cellular structures.
32182593	3	54	theme	large	529:533	arg1	printouts					561:569	large, volumetric multi-layered printouts	529:569	large, volumetric multi-layered printouts	529:569	As such, they generally possess weak mechanical properties and thus must be supported during fabrication in order to prevent the collapse of large, volumetric multi-layered printouts.
32182593	2	55	from	substances	307:316	arg1	processes					327:335	these processes	321:335	these processes	321:335	The printing substances in these processes are mostly based on biomaterials and living cells.
32182593	1	56	theme	complexity	233:242	arg1	degree					223:228	a high degree	216:228	a high degree of complexity, fine architecture and heterogeneous composition	216:291	3D bioprinting may revolutionize the field of tissue engineering by allowing fabrication of bio-structures with a high degree of complexity, fine architecture and heterogeneous composition.
32182593	0	57	theme	volumetric	61:70	arg1	structures					92:101	volumetric cell-containing ECM structures	61:101	volumetric cell-containing ECM structures	61:101	Transparent support media for high resolution 3D printing of volumetric cell-containing ECM structures.
32182593	6	58	theme	delicate	1130:1137	arg1	procedures					1150:1159	delicate extraction procedures	1130:1159	delicate extraction procedures	1130:1159	This hybrid also presents an exclusive combination of desirable properties such as biocompatibility, high transparency, stability at a wide range of temperatures and amenability to delicate extraction procedures.
32182593	7	59	dep	prolonged	1333:1341	arg1	precise					1344:1350	precise	1344:1350	precise	1344:1350	Moreover, as fabrication of large, volumetric biological structures may require hours and even days to accomplish, we have demonstrated that the hybrid medium can support prolonged, precise printing for at least 18 h. All these qualities make it a promising support medium for 3D printing of tissues and organs.
32182593	7	60	theme	prolonged	1333:1341	arg1	printing					1352:1359	prolonged, precise printing for at least 18 h. All these qualities make it a promising support medium for 3D printing of tissues and organs	1333:1471	prolonged, precise printing for at least 18 h. All these qualities make it a promising support medium for 3D printing of tissues and organs	1333:1471	Moreover, as fabrication of large, volumetric biological structures may require hours and even days to accomplish, we have demonstrated that the hybrid medium can support prolonged, precise printing for at least 18 h. All these qualities make it a promising support medium for 3D printing of tissues and organs.
32182593	5	61	theme	complex	905:911	arg1	constructs					913:922	complex constructs	905:922	complex constructs	905:922	We show that a hybrid material, comprised of calcium-alginate nanoparticles and xanthan gum, presents superb qualities that enable printing at high resolution of down to 10 microns, allowing fabrication of complex constructs and cellular structures.
32182593	6	62	theme	temperatures	1098:1109	arg1	range					1089:1093	a wide range	1082:1093	a wide range of temperatures and amenability	1082:1125	This hybrid also presents an exclusive combination of desirable properties such as biocompatibility, high transparency, stability at a wide range of temperatures and amenability to delicate extraction procedures.
32182593	6	63	theme	high	1050:1053	arg1	biocompatibility					1032:1047	biocompatibility	1032:1047	biocompatibility	1032:1047	This hybrid also presents an exclusive combination of desirable properties such as biocompatibility, high transparency, stability at a wide range of temperatures and amenability to delicate extraction procedures.
32182593	6	63	theme	high	1050:1053	arg1	transparency					1055:1066	high transparency	1050:1066	high transparency	1050:1066	This hybrid also presents an exclusive combination of desirable properties such as biocompatibility, high transparency, stability at a wide range of temperatures and amenability to delicate extraction procedures.
32182593	1	64	theme	tissue	150:155	arg1	engineering					157:167	tissue engineering	150:167	tissue engineering	150:167	3D bioprinting may revolutionize the field of tissue engineering by allowing fabrication of bio-structures with a high degree of complexity, fine architecture and heterogeneous composition.
32182593	1	65	theme	fine	245:248	arg1	architecture					250:261	fine architecture	245:261	fine architecture	245:261	3D bioprinting may revolutionize the field of tissue engineering by allowing fabrication of bio-structures with a high degree of complexity, fine architecture and heterogeneous composition.
33491913	0	0	theme	High	107:110	arg1	Photostability					112:125	High Photostability	107:125	High Photostability	107:125	Luminescent Gold Nanocluster-Methylcellulose Composite Optical Fibers with Low Attenuation Coefficient and High Photostability.
33491913	9	1	theme	reported	1401:1408	arg1	fibers					1431:1436	the previously reported biopolymeric optical fibers	1386:1436	the previously reported biopolymeric optical fibers	1386:1436	The optical performance is on par with or even better than some of the previously reported biopolymeric optical fibers.
33491913	10	2	theme	photostability	1609:1622	arg1	combination					1443:1453	The combination	1439:1453	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability	1439:1622	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability makes the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors.
33491913	7	3	theme	rod-like	1004:1011	arg1	nanocrystals					1023:1034	rod-like cellulose nanocrystals	1004:1034	rod-like cellulose nanocrystals	1004:1034	Further, the addition of either luminescent gold nanoclusters, rod-like cellulose nanocrystals or gold nanocluster-cellulose nanocrystal hybrids into the MC matrix furnishes strong and ductile composite fibers.
33491913	2	4	from	incorporation	320:332	arg1	POFs					366:369	POFs	366:369	POFs	366:369	Notably, the incorporation of luminescent nanomaterials in POFs offers optical amplification and sensing for advanced nanophotonics.
33491913	8	5	theme	propagation	1277:1287	arg1	loss					1289:1292	a propagation loss	1275:1292	a propagation loss	1275:1292	Using cutback attenuation measurement, it is shown that the resulting fibers can act as short-distance optical fibers with a propagation loss as low as 1.47 dB cm-1 .
33491913	9	6	theme	biopolymeric	1410:1421	arg1	fibers					1431:1436	the previously reported biopolymeric optical fibers	1386:1436	the previously reported biopolymeric optical fibers	1386:1436	The optical performance is on par with or even better than some of the previously reported biopolymeric optical fibers.
33491913	5	7	theme	efficient	750:758	arg1	methods					801:807	efficient and environmentally acceptable extrusion methods	750:807	efficient and environmentally acceptable extrusion methods	750:807	Therefore, biopolymer-based optical fibers containing nontoxic luminescent nanomaterials are needed, with efficient and environmentally acceptable extrusion methods.
33491913	7	8	theme	gold	985:988	arg1	nanoclusters					990:1001	luminescent gold nanoclusters	973:1001	luminescent gold nanoclusters	973:1001	Further, the addition of either luminescent gold nanoclusters, rod-like cellulose nanocrystals or gold nanocluster-cellulose nanocrystal hybrids into the MC matrix furnishes strong and ductile composite fibers.
33491913	1	9	theme	numerous	269:276	arg1	applications					293:304	numerous short-distance applications	269:304	numerous short-distance applications	269:304	Because of their lightweight structure, flexibility, and immunity to electromagnetic interference, polymer optical fibers (POFs) are used in numerous short-distance applications.
33491913	9	10	theme	optical	1423:1429	arg1	fibers					1431:1436	the previously reported biopolymeric optical fibers	1386:1436	the previously reported biopolymeric optical fibers	1386:1436	The optical performance is on par with or even better than some of the previously reported biopolymeric optical fibers.
33491913	10	11	theme	strain	1519:1524	arg1	values					1526:1531	maximum strain values	1511:1531	maximum strain values up to 8.4 GPa and 52%	1511:1553	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability makes the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors.
33491913	7	12	theme	nanoclusters	990:1001	arg1	addition					954:961	the addition	950:961	the addition of either luminescent gold nanoclusters, rod-like cellulose nanocrystals or gold nanocluster-cellulose nanocrystal hybrids into the MC matrix	950:1103	Further, the addition of either luminescent gold nanoclusters, rod-like cellulose nanocrystals or gold nanocluster-cellulose nanocrystal hybrids into the MC matrix furnishes strong and ductile composite fibers.
33491913	1	13	theme	short-distance	278:291	arg1	applications					293:304	numerous short-distance applications	269:304	numerous short-distance applications	269:304	Because of their lightweight structure, flexibility, and immunity to electromagnetic interference, polymer optical fibers (POFs) are used in numerous short-distance applications.
33491913	7	14	theme	ductile	1126:1132	arg1	fibers					1144:1149	strong and ductile composite fibers	1115:1149	strong and ductile composite fibers	1115:1149	Further, the addition of either luminescent gold nanoclusters, rod-like cellulose nanocrystals or gold nanocluster-cellulose nanocrystal hybrids into the MC matrix furnishes strong and ductile composite fibers.
33491913	10	15	theme	mechanical	1468:1477	arg1	properties					1479:1488	excellent mechanical properties	1458:1488	excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively)	1458:1568	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability makes the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors.
33491913	5	16	theme	nontoxic	698:705	arg1	nanomaterials					719:731	nontoxic luminescent nanomaterials	698:731	nontoxic luminescent nanomaterials	698:731	Therefore, biopolymer-based optical fibers containing nontoxic luminescent nanomaterials are needed, with efficient and environmentally acceptable extrusion methods.
33491913	10	17	dep	properties	1479:1488	arg1	values					1526:1531	maximum strain values	1511:1531	maximum strain values up to 8.4 GPa and 52%	1511:1553	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability makes the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors.
33491913	10	17	dep	properties	1479:1488	arg1	modulus					1499:1505	Young's modulus	1491:1505	Young's modulus	1491:1505	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability makes the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors.
33491913	7	18	theme	cellulose	1013:1021	arg1	nanocrystals					1023:1034	rod-like cellulose nanocrystals	1004:1034	rod-like cellulose nanocrystals	1004:1034	Further, the addition of either luminescent gold nanoclusters, rod-like cellulose nanocrystals or gold nanocluster-cellulose nanocrystal hybrids into the MC matrix furnishes strong and ductile composite fibers.
33491913	7	19	theme	gold	1039:1042	arg1	hybrids					1078:1084	gold nanocluster-cellulose nanocrystal hybrids	1039:1084	gold nanocluster-cellulose nanocrystal hybrids	1039:1084	Further, the addition of either luminescent gold nanoclusters, rod-like cellulose nanocrystals or gold nanocluster-cellulose nanocrystal hybrids into the MC matrix furnishes strong and ductile composite fibers.
33491913	8	20	theme	1.47 dB	1304:1310	arg1	cm-1					1312:1315	1.47 dB cm-1	1304:1315	1.47 dB cm-1	1304:1315	Using cutback attenuation measurement, it is shown that the resulting fibers can act as short-distance optical fibers with a propagation loss as low as 1.47 dB cm-1 .
33491913	10	21	theme	attenuation	1575:1585	arg1	coefficient					1587:1597	low attenuation coefficient	1571:1597	low attenuation coefficient	1571:1597	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability makes the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors.
33491913	5	22	theme	luminescent	707:717	arg1	nanomaterials					719:731	nontoxic luminescent nanomaterials	698:731	nontoxic luminescent nanomaterials	698:731	Therefore, biopolymer-based optical fibers containing nontoxic luminescent nanomaterials are needed, with efficient and environmentally acceptable extrusion methods.
33491913	10	23	theme	Young	1491:1495	arg1	modulus					1499:1505	Young's modulus	1491:1505	Young's modulus	1491:1505	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability makes the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors.
33491913	7	24	theme	luminescent	973:983	arg1	nanoclusters					990:1001	luminescent gold nanoclusters	973:1001	luminescent gold nanoclusters	973:1001	Further, the addition of either luminescent gold nanoclusters, rod-like cellulose nanocrystals or gold nanocluster-cellulose nanocrystal hybrids into the MC matrix furnishes strong and ductile composite fibers.
33491913	10	25	theme	fibers	1653:1658	arg1	candidates					1670:1679	the MC-based composite fibers excellent candidates	1630:1679	the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors	1630:1726	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability makes the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors.
33491913	4	26	theme	luminescent	556:566	arg1	nanomaterials					568:580	the traditionally used luminescent nanomaterials	533:580	the traditionally used luminescent nanomaterials	533:580	Furthermore, the traditionally used luminescent nanomaterials undergo photobleaching, oxidation, and they can be cytotoxic.
33491913	10	27	theme	excellent	1458:1466	arg1	properties					1479:1488	excellent mechanical properties	1458:1488	excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively)	1458:1568	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability makes the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors.
33491913	7	28	theme	hybrids	1078:1084	arg1	addition					954:961	the addition	950:961	the addition of either luminescent gold nanoclusters, rod-like cellulose nanocrystals or gold nanocluster-cellulose nanocrystal hybrids into the MC matrix	950:1103	Further, the addition of either luminescent gold nanoclusters, rod-like cellulose nanocrystals or gold nanocluster-cellulose nanocrystal hybrids into the MC matrix furnishes strong and ductile composite fibers.
33491913	1	29	theme	electromagnetic	197:211	arg1	interference					213:224	electromagnetic interference	197:224	electromagnetic interference	197:224	Because of their lightweight structure, flexibility, and immunity to electromagnetic interference, polymer optical fibers (POFs) are used in numerous short-distance applications.
33491913	0	30	theme	Gold	12:15	arg1	Fibers					63:68	Luminescent Gold Nanocluster-Methylcellulose Composite Optical Fibers	0:68	Luminescent Gold Nanocluster-Methylcellulose Composite Optical Fibers with Low Attenuation Coefficient and High Photostability.	0:126	Luminescent Gold Nanocluster-Methylcellulose Composite Optical Fibers with Low Attenuation Coefficient and High Photostability.
33491913	4	31	theme	used	551:554	arg1	nanomaterials					568:580	the traditionally used luminescent nanomaterials	533:580	the traditionally used luminescent nanomaterials	533:580	Furthermore, the traditionally used luminescent nanomaterials undergo photobleaching, oxidation, and they can be cytotoxic.
33491913	8	32	theme	cutback	1158:1164	arg1	measurement					1178:1188	cutback attenuation measurement	1158:1188	cutback attenuation measurement	1158:1188	Using cutback attenuation measurement, it is shown that the resulting fibers can act as short-distance optical fibers with a propagation loss as low as 1.47 dB cm-1 .
33491913	2	33	theme	nanomaterials	349:361	arg1	incorporation					320:332	the incorporation	316:332	the incorporation of luminescent nanomaterials in POFs	316:369	Notably, the incorporation of luminescent nanomaterials in POFs offers optical amplification and sensing for advanced nanophotonics.
33491913	10	34	dep	8.4 GPa	1539:1545	arg1	up					1533:1534	up	1533:1534	up	1533:1534	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability makes the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors.
33491913	5	35	contain	containing	687:696	arg2	nanomaterials					719:731	nontoxic luminescent nanomaterials	698:731	nontoxic luminescent nanomaterials	698:731	Therefore, biopolymer-based optical fibers containing nontoxic luminescent nanomaterials are needed, with efficient and environmentally acceptable extrusion methods.
33491913	5	35	contain	containing	687:696	arg1	fibers					680:685	biopolymer-based optical fibers	655:685	biopolymer-based optical fibers containing nontoxic luminescent nanomaterials	655:731	Therefore, biopolymer-based optical fibers containing nontoxic luminescent nanomaterials are needed, with efficient and environmentally acceptable extrusion methods.
33491913	0	36	theme	Luminescent	0:10	arg1	Fibers					63:68	Luminescent Gold Nanocluster-Methylcellulose Composite Optical Fibers	0:68	Luminescent Gold Nanocluster-Methylcellulose Composite Optical Fibers with Low Attenuation Coefficient and High Photostability.	0:126	Luminescent Gold Nanocluster-Methylcellulose Composite Optical Fibers with Low Attenuation Coefficient and High Photostability.
33491913	5	37	theme	biopolymer-based	655:670	arg1	fibers					680:685	biopolymer-based optical fibers	655:685	biopolymer-based optical fibers containing nontoxic luminescent nanomaterials	655:731	Therefore, biopolymer-based optical fibers containing nontoxic luminescent nanomaterials are needed, with efficient and environmentally acceptable extrusion methods.
33491913	2	38	theme	luminescent	337:347	arg1	nanomaterials					349:361	luminescent nanomaterials	337:361	luminescent nanomaterials	337:361	Notably, the incorporation of luminescent nanomaterials in POFs offers optical amplification and sensing for advanced nanophotonics.
33491913	3	39	theme	nonsustainable	479:492	arg1	components					494:503	nonsustainable components	479:503	nonsustainable components	479:503	However, conventional POFs suffer from nonsustainable components and processes.
33491913	0	40	theme	Composite	45:53	arg1	Fibers					63:68	Luminescent Gold Nanocluster-Methylcellulose Composite Optical Fibers	0:68	Luminescent Gold Nanocluster-Methylcellulose Composite Optical Fibers with Low Attenuation Coefficient and High Photostability.	0:126	Luminescent Gold Nanocluster-Methylcellulose Composite Optical Fibers with Low Attenuation Coefficient and High Photostability.
33491913	7	41	theme	nanocluster-cellulose	1044:1064	arg1	hybrids					1078:1084	gold nanocluster-cellulose nanocrystal hybrids	1039:1084	gold nanocluster-cellulose nanocrystal hybrids	1039:1084	Further, the addition of either luminescent gold nanoclusters, rod-like cellulose nanocrystals or gold nanocluster-cellulose nanocrystal hybrids into the MC matrix furnishes strong and ductile composite fibers.
33491913	10	42	theme	high	1604:1607	arg1	photostability					1609:1622	high photostability	1604:1622	high photostability	1604:1622	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability makes the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors.
33491913	5	43	theme	optical	672:678	arg1	fibers					680:685	biopolymer-based optical fibers	655:685	biopolymer-based optical fibers containing nontoxic luminescent nanomaterials	655:731	Therefore, biopolymer-based optical fibers containing nontoxic luminescent nanomaterials are needed, with efficient and environmentally acceptable extrusion methods.
33491913	1	44	theme	polymer	227:233	arg1	POFs					251:254	POFs	251:254	POFs	251:254	Because of their lightweight structure, flexibility, and immunity to electromagnetic interference, polymer optical fibers (POFs) are used in numerous short-distance applications.
33491913	1	44	theme	polymer	227:233	arg1	fibers					243:248	polymer optical fibers	227:248	polymer optical fibers (POFs)	227:255	Because of their lightweight structure, flexibility, and immunity to electromagnetic interference, polymer optical fibers (POFs) are used in numerous short-distance applications.
33491913	0	45	theme	Nanocluster-Methylcellulose	17:43	arg1	Fibers					63:68	Luminescent Gold Nanocluster-Methylcellulose Composite Optical Fibers	0:68	Luminescent Gold Nanocluster-Methylcellulose Composite Optical Fibers with Low Attenuation Coefficient and High Photostability.	0:126	Luminescent Gold Nanocluster-Methylcellulose Composite Optical Fibers with Low Attenuation Coefficient and High Photostability.
33491913	10	46	theme	MC-based	1634:1641	arg1	candidates					1670:1679	the MC-based composite fibers excellent candidates	1630:1679	the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors	1630:1726	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability makes the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors.
33491913	8	47	theme	resulting	1212:1220	arg1	fibers					1222:1227	the resulting fibers	1208:1227	the resulting fibers	1208:1227	Using cutback attenuation measurement, it is shown that the resulting fibers can act as short-distance optical fibers with a propagation loss as low as 1.47 dB cm-1 .
33491913	8	47	theme	resulting	1212:1220	arg1	fibers					1263:1268	short-distance optical fibers	1240:1268	short-distance optical fibers with a propagation loss	1240:1292	Using cutback attenuation measurement, it is shown that the resulting fibers can act as short-distance optical fibers with a propagation loss as low as 1.47 dB cm-1 .
33491913	7	48	theme	MC	1095:1096	arg1	matrix					1098:1103	the MC matrix	1091:1103	the MC matrix	1091:1103	Further, the addition of either luminescent gold nanoclusters, rod-like cellulose nanocrystals or gold nanocluster-cellulose nanocrystal hybrids into the MC matrix furnishes strong and ductile composite fibers.
33491913	1	49	theme	optical	235:241	arg1	POFs					251:254	POFs	251:254	POFs	251:254	Because of their lightweight structure, flexibility, and immunity to electromagnetic interference, polymer optical fibers (POFs) are used in numerous short-distance applications.
33491913	1	49	theme	optical	235:241	arg1	fibers					243:248	polymer optical fibers	227:248	polymer optical fibers (POFs)	227:255	Because of their lightweight structure, flexibility, and immunity to electromagnetic interference, polymer optical fibers (POFs) are used in numerous short-distance applications.
33491913	10	50	theme	composite	1643:1651	arg1	candidates					1670:1679	the MC-based composite fibers excellent candidates	1630:1679	the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors	1630:1726	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability makes the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors.
33491913	8	51	theme	short-distance	1240:1253	arg1	fibers					1222:1227	the resulting fibers	1208:1227	the resulting fibers	1208:1227	Using cutback attenuation measurement, it is shown that the resulting fibers can act as short-distance optical fibers with a propagation loss as low as 1.47 dB cm-1 .
33491913	8	51	theme	short-distance	1240:1253	arg1	fibers					1263:1268	short-distance optical fibers	1240:1268	short-distance optical fibers with a propagation loss	1240:1292	Using cutback attenuation measurement, it is shown that the resulting fibers can act as short-distance optical fibers with a propagation loss as low as 1.47 dB cm-1 .
33491913	2	52	theme	advanced	416:423	arg1	nanophotonics					425:437	advanced nanophotonics	416:437	advanced nanophotonics	416:437	Notably, the incorporation of luminescent nanomaterials in POFs offers optical amplification and sensing for advanced nanophotonics.
33491913	0	53	theme	Optical	55:61	arg1	Fibers					63:68	Luminescent Gold Nanocluster-Methylcellulose Composite Optical Fibers	0:68	Luminescent Gold Nanocluster-Methylcellulose Composite Optical Fibers with Low Attenuation Coefficient and High Photostability.	0:126	Luminescent Gold Nanocluster-Methylcellulose Composite Optical Fibers with Low Attenuation Coefficient and High Photostability.
33491913	10	54	theme	coefficient	1587:1597	arg1	combination					1443:1453	The combination	1439:1453	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability	1439:1622	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability makes the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors.
33491913	10	55	theme	properties	1479:1488	arg1	combination					1443:1453	The combination	1439:1453	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability	1439:1622	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability makes the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors.
33491913	6	56	from	dispersions	887:897	arg1	wet-spun					844:851	wet-spun	844:851	wet-spun	844:851	Here, such an approach for fibers wet-spun from aqueous methylcellulose (MC) dispersions under ambient conditions is demonstrated.
33491913	0	57	theme	Low	75:77	arg1	Coefficient					91:101	Low Attenuation Coefficient	75:101	Low Attenuation Coefficient	75:101	Luminescent Gold Nanocluster-Methylcellulose Composite Optical Fibers with Low Attenuation Coefficient and High Photostability.
33491913	5	58	theme	acceptable	780:789	arg1	methods					801:807	efficient and environmentally acceptable extrusion methods	750:807	efficient and environmentally acceptable extrusion methods	750:807	Therefore, biopolymer-based optical fibers containing nontoxic luminescent nanomaterials are needed, with efficient and environmentally acceptable extrusion methods.
33491913	10	59	theme	excellent	1660:1668	arg1	candidates					1670:1679	the MC-based composite fibers excellent candidates	1630:1679	the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors	1630:1726	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability makes the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors.
33491913	6	60	theme	methylcellulose	866:880	arg1	dispersions					887:897	aqueous methylcellulose (MC) dispersions	858:897	aqueous methylcellulose (MC) dispersions under ambient conditions	858:922	Here, such an approach for fibers wet-spun from aqueous methylcellulose (MC) dispersions under ambient conditions is demonstrated.
33491913	2	61	theme	optical	378:384	arg1	amplification					386:398	optical amplification	378:398	optical amplification	378:398	Notably, the incorporation of luminescent nanomaterials in POFs offers optical amplification and sensing for advanced nanophotonics.
33491913	3	62	theme	conventional	449:460	arg1	POFs					462:465	conventional POFs	449:465	conventional POFs	449:465	However, conventional POFs suffer from nonsustainable components and processes.
33491913	5	63	theme	extrusion	791:799	arg1	methods					801:807	efficient and environmentally acceptable extrusion methods	750:807	efficient and environmentally acceptable extrusion methods	750:807	Therefore, biopolymer-based optical fibers containing nontoxic luminescent nanomaterials are needed, with efficient and environmentally acceptable extrusion methods.
33491913	1	64	used	used	261:264	arg2	POFs					251:254	POFs	251:254	POFs	251:254	Because of their lightweight structure, flexibility, and immunity to electromagnetic interference, polymer optical fibers (POFs) are used in numerous short-distance applications.
33491913	1	64	used	used	261:264	arg2	fibers					243:248	polymer optical fibers	227:248	polymer optical fibers (POFs)	227:255	Because of their lightweight structure, flexibility, and immunity to electromagnetic interference, polymer optical fibers (POFs) are used in numerous short-distance applications.
33491913	10	65	theme	low	1571:1573	arg1	coefficient					1587:1597	low attenuation coefficient	1571:1597	low attenuation coefficient	1571:1597	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability makes the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors.
33491913	6	66	theme	aqueous	858:864	arg1	MC					883:884	MC	883:884	MC	883:884	Here, such an approach for fibers wet-spun from aqueous methylcellulose (MC) dispersions under ambient conditions is demonstrated.
33491913	6	66	theme	aqueous	858:864	arg1	methylcellulose					866:880	aqueous methylcellulose	858:880	aqueous methylcellulose (MC) dispersions under ambient conditions	858:922	Here, such an approach for fibers wet-spun from aqueous methylcellulose (MC) dispersions under ambient conditions is demonstrated.
33491913	1	67	theme	lightweight	145:155	arg1	structure					157:165	their lightweight structure	139:165	their lightweight structure	139:165	Because of their lightweight structure, flexibility, and immunity to electromagnetic interference, polymer optical fibers (POFs) are used in numerous short-distance applications.
33491913	7	68	theme	nanocrystal	1066:1076	arg1	hybrids					1078:1084	gold nanocluster-cellulose nanocrystal hybrids	1039:1084	gold nanocluster-cellulose nanocrystal hybrids	1039:1084	Further, the addition of either luminescent gold nanoclusters, rod-like cellulose nanocrystals or gold nanocluster-cellulose nanocrystal hybrids into the MC matrix furnishes strong and ductile composite fibers.
33491913	9	69	theme	optical	1323:1329	arg1	performance					1331:1341	The optical performance	1319:1341	The optical performance	1319:1341	The optical performance is on par with or even better than some of the previously reported biopolymeric optical fibers.
33491913	10	70	theme	maximum	1511:1517	arg1	values					1526:1531	maximum strain values	1511:1531	maximum strain values up to 8.4 GPa and 52%	1511:1553	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability makes the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors.
33491913	7	71	theme	strong	1115:1120	arg1	fibers					1144:1149	strong and ductile composite fibers	1115:1149	strong and ductile composite fibers	1115:1149	Further, the addition of either luminescent gold nanoclusters, rod-like cellulose nanocrystals or gold nanocluster-cellulose nanocrystal hybrids into the MC matrix furnishes strong and ductile composite fibers.
33491913	8	72	theme	optical	1255:1261	arg1	fibers					1222:1227	the resulting fibers	1208:1227	the resulting fibers	1208:1227	Using cutback attenuation measurement, it is shown that the resulting fibers can act as short-distance optical fibers with a propagation loss as low as 1.47 dB cm-1 .
33491913	8	72	theme	optical	1255:1261	arg1	fibers					1263:1268	short-distance optical fibers	1240:1268	short-distance optical fibers with a propagation loss	1240:1292	Using cutback attenuation measurement, it is shown that the resulting fibers can act as short-distance optical fibers with a propagation loss as low as 1.47 dB cm-1 .
33491913	6	73	theme	wet-spun	844:851	arg1	fibers					837:842	fibers	837:842	fibers wet-spun from aqueous methylcellulose (MC) dispersions under ambient conditions	837:922	Here, such an approach for fibers wet-spun from aqueous methylcellulose (MC) dispersions under ambient conditions is demonstrated.
33491913	6	74	theme	ambient	905:911	arg1	conditions					913:922	ambient conditions	905:922	ambient conditions	905:922	Here, such an approach for fibers wet-spun from aqueous methylcellulose (MC) dispersions under ambient conditions is demonstrated.
33491913	7	75	theme	composite	1134:1142	arg1	fibers					1144:1149	strong and ductile composite fibers	1115:1149	strong and ductile composite fibers	1115:1149	Further, the addition of either luminescent gold nanoclusters, rod-like cellulose nanocrystals or gold nanocluster-cellulose nanocrystal hybrids into the MC matrix furnishes strong and ductile composite fibers.
33491913	10	76	theme	multifunctional	1685:1699	arg1	fibers					1709:1714	multifunctional optical fibers	1685:1714	multifunctional optical fibers	1685:1714	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability makes the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors.
33491913	7	77	theme	nanocrystals	1023:1034	arg1	addition					954:961	the addition	950:961	the addition of either luminescent gold nanoclusters, rod-like cellulose nanocrystals or gold nanocluster-cellulose nanocrystal hybrids into the MC matrix	950:1103	Further, the addition of either luminescent gold nanoclusters, rod-like cellulose nanocrystals or gold nanocluster-cellulose nanocrystal hybrids into the MC matrix furnishes strong and ductile composite fibers.
33491913	8	78	theme	attenuation	1166:1176	arg1	measurement					1178:1188	cutback attenuation measurement	1158:1188	cutback attenuation measurement	1158:1188	Using cutback attenuation measurement, it is shown that the resulting fibers can act as short-distance optical fibers with a propagation loss as low as 1.47 dB cm-1 .
33491913	0	79	theme	Attenuation	79:89	arg1	Coefficient					91:101	Low Attenuation Coefficient	75:101	Low Attenuation Coefficient	75:101	Luminescent Gold Nanocluster-Methylcellulose Composite Optical Fibers with Low Attenuation Coefficient and High Photostability.
33491913	0	80	with	Fibers	63:68	arg1	Coefficient					91:101	Low Attenuation Coefficient	75:101	Low Attenuation Coefficient	75:101	Luminescent Gold Nanocluster-Methylcellulose Composite Optical Fibers with Low Attenuation Coefficient and High Photostability.
33491913	0	80	with	Fibers	63:68	arg1	Photostability					112:125	High Photostability	107:125	High Photostability	107:125	Luminescent Gold Nanocluster-Methylcellulose Composite Optical Fibers with Low Attenuation Coefficient and High Photostability.
33491913	8	81	with	fibers	1263:1268	arg1	loss					1289:1292	a propagation loss	1275:1292	a propagation loss	1275:1292	Using cutback attenuation measurement, it is shown that the resulting fibers can act as short-distance optical fibers with a propagation loss as low as 1.47 dB cm-1 .
33491913	10	82	theme	optical	1701:1707	arg1	fibers					1709:1714	multifunctional optical fibers	1685:1714	multifunctional optical fibers	1685:1714	The combination of excellent mechanical properties (Young's modulus and maximum strain values up to 8.4 GPa and 52%, respectively), low attenuation coefficient, and high photostability makes the MC-based composite fibers excellent candidates for multifunctional optical fibers and sensors.
31980859	3	0	theme	subunit	717:723	arg1	compositions					725:736	low molecular weight subunit compositions	696:736	low molecular weight subunit compositions	696:736	The isolated multienzyme complexes here show similar particle size with the xylanosomes produced by Cellulosimicrobium cellulans F16, have at least two conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions.
31980859	4	1	theme	diverse	888:894	arg1	capability					896:905	its diverse capability	884:905	its diverse capability	884:905	This is the first report of the enzymatic activities and subunit structures of xylanosome produced by Oerskovia turbata, providing insights into its diverse capability as well as degrading bias on hemicelluloses.
31980859	1	2	theme	functional	140:149	arg1	studies					151:157	functional studies	140:157	functional studies of the multienzyme complexes produced by Oerskovia turbata JCM 3160 and reveal of their subunit structures	140:264	This work aims at functional studies of the multienzyme complexes produced by Oerskovia turbata JCM 3160 and reveal of their subunit structures.
31980859	0	3	theme	Xylanosomes	70:80	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, Substrate Specificity, and Subunit Characterization of the Xylanosomes Produced by Oerskovia turbata JCM 3160.
31980859	0	3	theme	Xylanosomes	70:80	arg1	Specificity					21:31	Substrate Specificity	11:31	Substrate Specificity	11:31	Isolation, Substrate Specificity, and Subunit Characterization of the Xylanosomes Produced by Oerskovia turbata JCM 3160.
31980859	0	3	theme	Xylanosomes	70:80	arg1	Characterization					46:61	Subunit Characterization	38:61	Subunit Characterization	38:61	Isolation, Substrate Specificity, and Subunit Characterization of the Xylanosomes Produced by Oerskovia turbata JCM 3160.
31980859	3	4	theme	low	696:698	arg1	compositions					725:736	low molecular weight subunit compositions	696:736	low molecular weight subunit compositions	696:736	The isolated multienzyme complexes here show similar particle size with the xylanosomes produced by Cellulosimicrobium cellulans F16, have at least two conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions.
31980859	2	5	theme	genome	337:342	arg1	sequence					344:351	the whole genome sequence	327:351	the whole genome sequence	327:351	The multienzyme complexes were isolated, enzymatic assayed, the whole genome sequence was determined in fine scale, and the subunit structure was identified by Maldi-TOF mass spectrometry.
31980859	0	6	theme	Oerskovia	94:102	arg1	3160					116:119	Oerskovia turbata JCM 3160	94:119	Oerskovia turbata JCM 3160	94:119	Isolation, Substrate Specificity, and Subunit Characterization of the Xylanosomes Produced by Oerskovia turbata JCM 3160.
31980859	3	7	theme	molecular	700:708	arg1	compositions					725:736	low molecular weight subunit compositions	696:736	low molecular weight subunit compositions	696:736	The isolated multienzyme complexes here show similar particle size with the xylanosomes produced by Cellulosimicrobium cellulans F16, have at least two conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions.
31980859	4	8	theme	enzymatic	771:779	arg1	activities					781:790	enzymatic activities	771:790	enzymatic activities	771:790	This is the first report of the enzymatic activities and subunit structures of xylanosome produced by Oerskovia turbata, providing insights into its diverse capability as well as degrading bias on hemicelluloses.
31980859	1	9	theme	subunit	247:253	arg1	structures					255:264	their subunit structures	241:264	their subunit structures	241:264	This work aims at functional studies of the multienzyme complexes produced by Oerskovia turbata JCM 3160 and reveal of their subunit structures.
31980859	2	10	theme	multienzyme	271:281	arg1	complexes					283:291	The multienzyme complexes	267:291	The multienzyme complexes	267:291	The multienzyme complexes were isolated, enzymatic assayed, the whole genome sequence was determined in fine scale, and the subunit structure was identified by Maldi-TOF mass spectrometry.
31980859	1	11	theme	structures	255:264	arg1	3160					222:225	Oerskovia turbata JCM 3160	200:225	Oerskovia turbata JCM 3160	200:225	This work aims at functional studies of the multienzyme complexes produced by Oerskovia turbata JCM 3160 and reveal of their subunit structures.
31980859	0	12	theme	JCM	112:114	arg1	3160					116:119	Oerskovia turbata JCM 3160	94:119	Oerskovia turbata JCM 3160	94:119	Isolation, Substrate Specificity, and Subunit Characterization of the Xylanosomes Produced by Oerskovia turbata JCM 3160.
31980859	0	13	dep	Oerskovia	94:102	arg1	turbata					104:110	turbata	104:110	turbata	104:110	Isolation, Substrate Specificity, and Subunit Characterization of the Xylanosomes Produced by Oerskovia turbata JCM 3160.
31980859	1	14	theme	multienzyme	166:176	arg1	complexes					178:186	the multienzyme complexes	162:186	the multienzyme complexes produced by Oerskovia turbata JCM 3160 and reveal of their subunit structures	162:264	This work aims at functional studies of the multienzyme complexes produced by Oerskovia turbata JCM 3160 and reveal of their subunit structures.
31980859	2	15	theme	mass	437:440	arg1	spectrometry					442:453	Maldi-TOF mass spectrometry	427:453	Maldi-TOF mass spectrometry	427:453	The multienzyme complexes were isolated, enzymatic assayed, the whole genome sequence was determined in fine scale, and the subunit structure was identified by Maldi-TOF mass spectrometry.
31980859	4	16	theme	activities	781:790	arg1	report					757:762	the first report	747:762	the first report of the enzymatic activities and subunit structures of xylanosome produced by Oerskovia turbata	747:857	This is the first report of the enzymatic activities and subunit structures of xylanosome produced by Oerskovia turbata, providing insights into its diverse capability as well as degrading bias on hemicelluloses.
31980859	4	16	theme	activities	781:790	arg1	This					739:742	This	739:742	This	739:742	This is the first report of the enzymatic activities and subunit structures of xylanosome produced by Oerskovia turbata, providing insights into its diverse capability as well as degrading bias on hemicelluloses.
31980859	2	17	theme	Maldi-TOF	427:435	arg1	spectrometry					442:453	Maldi-TOF mass spectrometry	427:453	Maldi-TOF mass spectrometry	427:453	The multienzyme complexes were isolated, enzymatic assayed, the whole genome sequence was determined in fine scale, and the subunit structure was identified by Maldi-TOF mass spectrometry.
31980859	4	18	theme	first	751:755	arg1	report					757:762	the first report	747:762	the first report of the enzymatic activities and subunit structures of xylanosome produced by Oerskovia turbata	747:857	This is the first report of the enzymatic activities and subunit structures of xylanosome produced by Oerskovia turbata, providing insights into its diverse capability as well as degrading bias on hemicelluloses.
31980859	4	18	theme	first	751:755	arg1	This					739:742	This	739:742	This	739:742	This is the first report of the enzymatic activities and subunit structures of xylanosome produced by Oerskovia turbata, providing insights into its diverse capability as well as degrading bias on hemicelluloses.
31980859	2	19	theme	fine	371:374	arg1	scale					376:380	fine scale	371:380	fine scale	371:380	The multienzyme complexes were isolated, enzymatic assayed, the whole genome sequence was determined in fine scale, and the subunit structure was identified by Maldi-TOF mass spectrometry.
31980859	1	20	theme	complexes	178:186	arg1	studies					151:157	functional studies	140:157	functional studies of the multienzyme complexes produced by Oerskovia turbata JCM 3160 and reveal of their subunit structures	140:264	This work aims at functional studies of the multienzyme complexes produced by Oerskovia turbata JCM 3160 and reveal of their subunit structures.
31980859	0	21	theme	Substrate	11:19	arg1	Specificity					21:31	Substrate Specificity	11:31	Substrate Specificity	11:31	Isolation, Substrate Specificity, and Subunit Characterization of the Xylanosomes Produced by Oerskovia turbata JCM 3160.
31980859	3	22	theme	similar	501:507	arg1	size					518:521	similar particle size	501:521	similar particle size	501:521	The isolated multienzyme complexes here show similar particle size with the xylanosomes produced by Cellulosimicrobium cellulans F16, have at least two conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions.
31980859	3	23	dep	F16	585:587	arg1	have					590:593	have	590:593	have	590:593	The isolated multienzyme complexes here show similar particle size with the xylanosomes produced by Cellulosimicrobium cellulans F16, have at least two conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions.
31980859	2	24	theme	subunit	391:397	arg1	structure					399:407	the subunit structure	387:407	the subunit structure	387:407	The multienzyme complexes were isolated, enzymatic assayed, the whole genome sequence was determined in fine scale, and the subunit structure was identified by Maldi-TOF mass spectrometry.
31980859	1	25	theme	Oerskovia	200:208	arg1	3160					222:225	Oerskovia turbata JCM 3160	200:225	Oerskovia turbata JCM 3160	200:225	This work aims at functional studies of the multienzyme complexes produced by Oerskovia turbata JCM 3160 and reveal of their subunit structures.
31980859	1	26	theme	turbata	210:216	arg1	3160					222:225	Oerskovia turbata JCM 3160	200:225	Oerskovia turbata JCM 3160	200:225	This work aims at functional studies of the multienzyme complexes produced by Oerskovia turbata JCM 3160 and reveal of their subunit structures.
31980859	2	27	theme	whole	331:335	arg1	sequence					344:351	the whole genome sequence	327:351	the whole genome sequence	327:351	The multienzyme complexes were isolated, enzymatic assayed, the whole genome sequence was determined in fine scale, and the subunit structure was identified by Maldi-TOF mass spectrometry.
31980859	0	28	theme	Subunit	38:44	arg1	Characterization					46:61	Subunit Characterization	38:61	Subunit Characterization	38:61	Isolation, Substrate Specificity, and Subunit Characterization of the Xylanosomes Produced by Oerskovia turbata JCM 3160.
31980859	4	29	theme	Oerskovia	841:849	arg1	turbata					851:857	Oerskovia turbata	841:857	Oerskovia turbata	841:857	This is the first report of the enzymatic activities and subunit structures of xylanosome produced by Oerskovia turbata, providing insights into its diverse capability as well as degrading bias on hemicelluloses.
31980859	4	30	theme	structures	804:813	arg1	report					757:762	the first report	747:762	the first report of the enzymatic activities and subunit structures of xylanosome produced by Oerskovia turbata	747:857	This is the first report of the enzymatic activities and subunit structures of xylanosome produced by Oerskovia turbata, providing insights into its diverse capability as well as degrading bias on hemicelluloses.
31980859	4	30	theme	structures	804:813	arg1	This					739:742	This	739:742	This	739:742	This is the first report of the enzymatic activities and subunit structures of xylanosome produced by Oerskovia turbata, providing insights into its diverse capability as well as degrading bias on hemicelluloses.
31980859	3	31	theme	multi-domain	618:629	arg1	proteins					631:638	at least two conserved multi-domain proteins	595:638	at least two conserved multi-domain proteins	595:638	The isolated multienzyme complexes here show similar particle size with the xylanosomes produced by Cellulosimicrobium cellulans F16, have at least two conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions.
31980859	3	32	theme	isolated	460:467	arg1	complexes					481:489	The isolated multienzyme complexes	456:489	The isolated multienzyme complexes	456:489	The isolated multienzyme complexes here show similar particle size with the xylanosomes produced by Cellulosimicrobium cellulans F16, have at least two conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions.
31980859	1	33	theme	JCM	218:220	arg1	3160					222:225	Oerskovia turbata JCM 3160	200:225	Oerskovia turbata JCM 3160	200:225	This work aims at functional studies of the multienzyme complexes produced by Oerskovia turbata JCM 3160 and reveal of their subunit structures.
31980859	4	34	theme	subunit	796:802	arg1	structures					804:813	subunit structures	796:813	subunit structures of xylanosome	796:827	This is the first report of the enzymatic activities and subunit structures of xylanosome produced by Oerskovia turbata, providing insights into its diverse capability as well as degrading bias on hemicelluloses.
31980859	3	35	theme	multienzyme	469:479	arg1	complexes					481:489	The isolated multienzyme complexes	456:489	The isolated multienzyme complexes	456:489	The isolated multienzyme complexes here show similar particle size with the xylanosomes produced by Cellulosimicrobium cellulans F16, have at least two conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions.
31980859	3	36	theme	enzymatic	671:679	arg1	activities					681:690	enzymatic activities	671:690	enzymatic activities	671:690	The isolated multienzyme complexes here show similar particle size with the xylanosomes produced by Cellulosimicrobium cellulans F16, have at least two conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions.
31980859	4	37	theme	xylanosome	818:827	arg1	activities					781:790	enzymatic activities	771:790	enzymatic activities	771:790	This is the first report of the enzymatic activities and subunit structures of xylanosome produced by Oerskovia turbata, providing insights into its diverse capability as well as degrading bias on hemicelluloses.
31980859	4	37	theme	xylanosome	818:827	arg1	structures					804:813	subunit structures	796:813	subunit structures of xylanosome	796:827	This is the first report of the enzymatic activities and subunit structures of xylanosome produced by Oerskovia turbata, providing insights into its diverse capability as well as degrading bias on hemicelluloses.
31980859	3	38	theme	particle	509:516	arg1	size					518:521	similar particle size	501:521	similar particle size	501:521	The isolated multienzyme complexes here show similar particle size with the xylanosomes produced by Cellulosimicrobium cellulans F16, have at least two conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions.
31980859	3	39	theme	weight	710:715	arg1	compositions					725:736	low molecular weight subunit compositions	696:736	low molecular weight subunit compositions	696:736	The isolated multienzyme complexes here show similar particle size with the xylanosomes produced by Cellulosimicrobium cellulans F16, have at least two conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions.
31980859	3	40	theme	conserved	608:616	arg1	proteins					631:638	at least two conserved multi-domain proteins	595:638	at least two conserved multi-domain proteins	595:638	The isolated multienzyme complexes here show similar particle size with the xylanosomes produced by Cellulosimicrobium cellulans F16, have at least two conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions.
31980859	4	41	dep	activities	781:790	arg1	the					767:769	the	767:769	the	767:769	This is the first report of the enzymatic activities and subunit structures of xylanosome produced by Oerskovia turbata, providing insights into its diverse capability as well as degrading bias on hemicelluloses.
32536404	7	0	theme	field	1146:1150	arg1	microscopy					1179:1188	field emission scanning electron microscopy	1146:1188	field emission scanning electron microscopy	1146:1188	Surface morphology by field emission scanning electron microscopy reveals the presence of protrusions in fiber which aid in the better adhesion with the matrix in composite manufacturing.
32536404	2	1	with	line	270:273	arg1	this					280:283	this	280:283	this	280:283	In line with this, a novel cellulose fiber was extracted from the Kigelia africana fruit and its physical, chemical and thermal properties, crystallography and surface morphology analysis were studied and reported in this investigative research paper.
32536404	2	2	theme	morphology	435:444	arg1	properties					395:404	its physical, chemical and thermal properties	360:404	its physical, chemical and thermal properties	360:404	In line with this, a novel cellulose fiber was extracted from the Kigelia africana fruit and its physical, chemical and thermal properties, crystallography and surface morphology analysis were studied and reported in this investigative research paper.
32536404	2	2	theme	morphology	435:444	arg1	analysis					446:453	surface morphology analysis	427:453	surface morphology analysis	427:453	In line with this, a novel cellulose fiber was extracted from the Kigelia africana fruit and its physical, chemical and thermal properties, crystallography and surface morphology analysis were studied and reported in this investigative research paper.
32536404	1	3	theme	increasing	130:139	arg1	use					141:143	an increasing use	127:143	an increasing use of green composites in composite manufacturing, where cellulosic natural fibers have been started using for this purpose	127:264	In recent days, there is an increasing use of green composites in composite manufacturing, where cellulosic natural fibers have been started using for this purpose.
32536404	0	4	from	Study	0:4	arg1	fiber					36:40	a Novel natural cellulosic fiber	9:40	a Novel natural cellulosic fiber from Kigelia africana fruit	9:68	Study on a Novel natural cellulosic fiber from Kigelia africana fruit: Characterization and analysis.
32536404	3	5	theme	0.182	652:656	arg1	mm					658:659	mm	658:659	mm	658:659	The physical analysis revealed the mean tensile strength as 50.31 ± 24.71 to 73.12 ± 32.48 MPa, diameter as 0.507 ± 0.162 to 0.629 ± 0.182 mm and density as 1.316 g/cm³ for the Kigelia africana fiber.
32536404	4	6	dep	transform	914:922	arg1	infrared					924:931	infrared	924:931	transform infrared spectroscopy analysis	914:953	The proximate chemical analysis estimated the cellulose percentage to be 61.5 % and the existence of different basic components like cellulose, hemicellulose and lignin are confirmed by Fourier transform infrared spectroscopy analysis.
32536404	6	7	theme	X-ray	1105:1109	arg1	diffraction					1111:1121	X-ray diffraction	1105:1121	X-ray diffraction	1105:1121	The crystallinity index, 57.38 % of the fiber was determined by X-ray diffraction.
32536404	3	8	theme	mm	658:659	arg1	diameter					615:622	diameter	615:622	diameter as 0.507 ± 0.162 to 0.629 ± 0.182 mm	615:659	The physical analysis revealed the mean tensile strength as 50.31 ± 24.71 to 73.12 ± 32.48 MPa, diameter as 0.507 ± 0.162 to 0.629 ± 0.182 mm and density as 1.316 g/cm³ for the Kigelia africana fiber.
32536404	4	9	theme	cellulose	766:774	arg1	percentage					776:785	the cellulose percentage	762:785	the cellulose percentage to be 61.5 % and the existence of different basic components like cellulose, hemicellulose and lignin are confirmed by Fourier transform infrared spectroscopy analysis	762:953	The proximate chemical analysis estimated the cellulose percentage to be 61.5 % and the existence of different basic components like cellulose, hemicellulose and lignin are confirmed by Fourier transform infrared spectroscopy analysis.
32536404	2	10	theme	physical	364:371	arg1	crystallography					407:421	crystallography	407:421	crystallography	407:421	In line with this, a novel cellulose fiber was extracted from the Kigelia africana fruit and its physical, chemical and thermal properties, crystallography and surface morphology analysis were studied and reported in this investigative research paper.
32536404	2	10	theme	physical	364:371	arg1	properties					395:404	its physical, chemical and thermal properties	360:404	its physical, chemical and thermal properties	360:404	In line with this, a novel cellulose fiber was extracted from the Kigelia africana fruit and its physical, chemical and thermal properties, crystallography and surface morphology analysis were studied and reported in this investigative research paper.
32536404	2	10	theme	physical	364:371	arg1	analysis					446:453	surface morphology analysis	427:453	surface morphology analysis	427:453	In line with this, a novel cellulose fiber was extracted from the Kigelia africana fruit and its physical, chemical and thermal properties, crystallography and surface morphology analysis were studied and reported in this investigative research paper.
32536404	2	11	theme	Kigelia	333:339	arg1	fruit					350:354	the Kigelia africana fruit	329:354	the Kigelia africana fruit	329:354	In line with this, a novel cellulose fiber was extracted from the Kigelia africana fruit and its physical, chemical and thermal properties, crystallography and surface morphology analysis were studied and reported in this investigative research paper.
32536404	4	12	theme	spectroscopy	933:944	arg1	analysis					946:953	spectroscopy analysis	933:953	spectroscopy analysis	933:953	The proximate chemical analysis estimated the cellulose percentage to be 61.5 % and the existence of different basic components like cellulose, hemicellulose and lignin are confirmed by Fourier transform infrared spectroscopy analysis.
32536404	2	13	theme	surface	427:433	arg1	properties					395:404	its physical, chemical and thermal properties	360:404	its physical, chemical and thermal properties	360:404	In line with this, a novel cellulose fiber was extracted from the Kigelia africana fruit and its physical, chemical and thermal properties, crystallography and surface morphology analysis were studied and reported in this investigative research paper.
32536404	2	13	theme	surface	427:433	arg1	analysis					446:453	surface morphology analysis	427:453	surface morphology analysis	427:453	In line with this, a novel cellulose fiber was extracted from the Kigelia africana fruit and its physical, chemical and thermal properties, crystallography and surface morphology analysis were studied and reported in this investigative research paper.
32536404	3	14	dep	73.12	596:600	arg1	to					593:594	to	593:594	to	593:594	The physical analysis revealed the mean tensile strength as 50.31 ± 24.71 to 73.12 ± 32.48 MPa, diameter as 0.507 ± 0.162 to 0.629 ± 0.182 mm and density as 1.316 g/cm³ for the Kigelia africana fiber.
32536404	5	15	theme	Thermogravimetric	956:972	arg1	analysis					974:981	Thermogravimetric analysis	956:981	Thermogravimetric analysis	956:981	Thermogravimetric analysis establishes the thermal stability of the fiber as 212 ⁰C.
32536404	7	16	theme	emission	1152:1159	arg1	microscopy					1179:1188	field emission scanning electron microscopy	1146:1188	field emission scanning electron microscopy	1146:1188	Surface morphology by field emission scanning electron microscopy reveals the presence of protrusions in fiber which aid in the better adhesion with the matrix in composite manufacturing.
32536404	7	17	theme	scanning	1161:1168	arg1	microscopy					1179:1188	field emission scanning electron microscopy	1146:1188	field emission scanning electron microscopy	1146:1188	Surface morphology by field emission scanning electron microscopy reveals the presence of protrusions in fiber which aid in the better adhesion with the matrix in composite manufacturing.
32536404	1	18	theme	green	148:152	arg1	composites					154:163	green composites	148:163	green composites	148:163	In recent days, there is an increasing use of green composites in composite manufacturing, where cellulosic natural fibers have been started using for this purpose.
32536404	2	19	theme	thermal	387:393	arg1	crystallography					407:421	crystallography	407:421	crystallography	407:421	In line with this, a novel cellulose fiber was extracted from the Kigelia africana fruit and its physical, chemical and thermal properties, crystallography and surface morphology analysis were studied and reported in this investigative research paper.
32536404	2	19	theme	thermal	387:393	arg1	properties					395:404	its physical, chemical and thermal properties	360:404	its physical, chemical and thermal properties	360:404	In line with this, a novel cellulose fiber was extracted from the Kigelia africana fruit and its physical, chemical and thermal properties, crystallography and surface morphology analysis were studied and reported in this investigative research paper.
32536404	2	19	theme	thermal	387:393	arg1	analysis					446:453	surface morphology analysis	427:453	surface morphology analysis	427:453	In line with this, a novel cellulose fiber was extracted from the Kigelia africana fruit and its physical, chemical and thermal properties, crystallography and surface morphology analysis were studied and reported in this investigative research paper.
32536404	6	20	theme	fiber	1081:1085	arg1	index					1059:1063	The crystallinity index	1041:1063	The crystallinity index	1041:1063	The crystallinity index, 57.38 % of the fiber was determined by X-ray diffraction.
32536404	6	20	theme	fiber	1081:1085	arg1	%					1072:1072	57.38 %	1066:1072	57.38 %	1066:1072	The crystallinity index, 57.38 % of the fiber was determined by X-ray diffraction.
32536404	1	21	theme	composites	154:163	arg1	use					141:143	an increasing use	127:143	an increasing use of green composites in composite manufacturing, where cellulosic natural fibers have been started using for this purpose	127:264	In recent days, there is an increasing use of green composites in composite manufacturing, where cellulosic natural fibers have been started using for this purpose.
32536404	0	22	theme	Novel	11:15	arg1	fiber					36:40	a Novel natural cellulosic fiber	9:40	a Novel natural cellulosic fiber from Kigelia africana fruit	9:68	Study on a Novel natural cellulosic fiber from Kigelia africana fruit: Characterization and analysis.
32536404	4	23	theme	components	837:846	arg1	components					837:846	different basic components	821:846	different basic components	821:846	The proximate chemical analysis estimated the cellulose percentage to be 61.5 % and the existence of different basic components like cellulose, hemicellulose and lignin are confirmed by Fourier transform infrared spectroscopy analysis.
32536404	4	23	theme	components	837:846	arg1	%					798:798	61.5 %	793:798	61.5 %	793:798	The proximate chemical analysis estimated the cellulose percentage to be 61.5 % and the existence of different basic components like cellulose, hemicellulose and lignin are confirmed by Fourier transform infrared spectroscopy analysis.
32536404	4	23	theme	components	837:846	arg1	existence					808:816	the existence	804:816	the existence of different basic components	804:846	The proximate chemical analysis estimated the cellulose percentage to be 61.5 % and the existence of different basic components like cellulose, hemicellulose and lignin are confirmed by Fourier transform infrared spectroscopy analysis.
32536404	7	24	from	presence	1202:1209	arg1	fiber					1229:1233	fiber	1229:1233	fiber	1229:1233	Surface morphology by field emission scanning electron microscopy reveals the presence of protrusions in fiber which aid in the better adhesion with the matrix in composite manufacturing.
32536404	4	25	theme	chemical	734:741	arg1	analysis					743:750	The proximate chemical analysis	720:750	The proximate chemical analysis	720:750	The proximate chemical analysis estimated the cellulose percentage to be 61.5 % and the existence of different basic components like cellulose, hemicellulose and lignin are confirmed by Fourier transform infrared spectroscopy analysis.
32536404	2	26	theme	chemical	374:381	arg1	crystallography					407:421	crystallography	407:421	crystallography	407:421	In line with this, a novel cellulose fiber was extracted from the Kigelia africana fruit and its physical, chemical and thermal properties, crystallography and surface morphology analysis were studied and reported in this investigative research paper.
32536404	2	26	theme	chemical	374:381	arg1	properties					395:404	its physical, chemical and thermal properties	360:404	its physical, chemical and thermal properties	360:404	In line with this, a novel cellulose fiber was extracted from the Kigelia africana fruit and its physical, chemical and thermal properties, crystallography and surface morphology analysis were studied and reported in this investigative research paper.
32536404	2	26	theme	chemical	374:381	arg1	analysis					446:453	surface morphology analysis	427:453	surface morphology analysis	427:453	In line with this, a novel cellulose fiber was extracted from the Kigelia africana fruit and its physical, chemical and thermal properties, crystallography and surface morphology analysis were studied and reported in this investigative research paper.
32536404	4	27	theme	basic	831:835	arg1	components					837:846	different basic components	821:846	different basic components	821:846	The proximate chemical analysis estimated the cellulose percentage to be 61.5 % and the existence of different basic components like cellulose, hemicellulose and lignin are confirmed by Fourier transform infrared spectroscopy analysis.
32536404	3	28	theme	±	602:602	arg1	MPa					610:612	± 32.48 MPa	602:612	± 32.48 MPa	602:612	The physical analysis revealed the mean tensile strength as 50.31 ± 24.71 to 73.12 ± 32.48 MPa, diameter as 0.507 ± 0.162 to 0.629 ± 0.182 mm and density as 1.316 g/cm³ for the Kigelia africana fiber.
32536404	3	29	theme	physical	523:530	arg1	analysis					532:539	The physical analysis	519:539	The physical analysis	519:539	The physical analysis revealed the mean tensile strength as 50.31 ± 24.71 to 73.12 ± 32.48 MPa, diameter as 0.507 ± 0.162 to 0.629 ± 0.182 mm and density as 1.316 g/cm³ for the Kigelia africana fiber.
32536404	1	30	theme	composite	168:176	arg1	manufacturing					178:190	composite manufacturing	168:190	composite manufacturing	168:190	In recent days, there is an increasing use of green composites in composite manufacturing, where cellulosic natural fibers have been started using for this purpose.
32536404	0	31	theme	cellulosic	25:34	arg1	fiber					36:40	a Novel natural cellulosic fiber	9:40	a Novel natural cellulosic fiber from Kigelia africana fruit	9:68	Study on a Novel natural cellulosic fiber from Kigelia africana fruit: Characterization and analysis.
32536404	2	32	theme	novel	288:292	arg1	fiber					304:308	a novel cellulose fiber	286:308	a novel cellulose fiber	286:308	In line with this, a novel cellulose fiber was extracted from the Kigelia africana fruit and its physical, chemical and thermal properties, crystallography and surface morphology analysis were studied and reported in this investigative research paper.
32536404	2	33	theme	research	503:510	arg1	paper					512:516	this investigative research paper	484:516	this investigative research paper	484:516	In line with this, a novel cellulose fiber was extracted from the Kigelia africana fruit and its physical, chemical and thermal properties, crystallography and surface morphology analysis were studied and reported in this investigative research paper.
32536404	0	34	from	fruit	64:68	arg1	fiber					36:40	a Novel natural cellulosic fiber	9:40	a Novel natural cellulosic fiber from Kigelia africana fruit	9:68	Study on a Novel natural cellulosic fiber from Kigelia africana fruit: Characterization and analysis.
32536404	3	35	theme	tensile	559:565	arg1	strength					567:574	the mean tensile strength	550:574	the mean tensile strength	550:574	The physical analysis revealed the mean tensile strength as 50.31 ± 24.71 to 73.12 ± 32.48 MPa, diameter as 0.507 ± 0.162 to 0.629 ± 0.182 mm and density as 1.316 g/cm³ for the Kigelia africana fiber.
32536404	3	36	dep	±	585:585	arg1	MPa					610:612	± 32.48 MPa	602:612	± 32.48 MPa	602:612	The physical analysis revealed the mean tensile strength as 50.31 ± 24.71 to 73.12 ± 32.48 MPa, diameter as 0.507 ± 0.162 to 0.629 ± 0.182 mm and density as 1.316 g/cm³ for the Kigelia africana fiber.
32536404	0	37	theme	natural	17:23	arg1	fiber					36:40	a Novel natural cellulosic fiber	9:40	a Novel natural cellulosic fiber from Kigelia africana fruit	9:68	Study on a Novel natural cellulosic fiber from Kigelia africana fruit: Characterization and analysis.
32536404	3	38	dep	0.629	644:648	arg1	to					641:642	to	641:642	to	641:642	The physical analysis revealed the mean tensile strength as 50.31 ± 24.71 to 73.12 ± 32.48 MPa, diameter as 0.507 ± 0.162 to 0.629 ± 0.182 mm and density as 1.316 g/cm³ for the Kigelia africana fiber.
32536404	2	39	theme	investigative	489:501	arg1	paper					512:516	this investigative research paper	484:516	this investigative research paper	484:516	In line with this, a novel cellulose fiber was extracted from the Kigelia africana fruit and its physical, chemical and thermal properties, crystallography and surface morphology analysis were studied and reported in this investigative research paper.
32536404	3	40	theme	africana	704:711	arg1	fiber					713:717	the Kigelia africana fiber	692:717	the Kigelia africana fiber	692:717	The physical analysis revealed the mean tensile strength as 50.31 ± 24.71 to 73.12 ± 32.48 MPa, diameter as 0.507 ± 0.162 to 0.629 ± 0.182 mm and density as 1.316 g/cm³ for the Kigelia africana fiber.
32536404	7	41	theme	Surface	1124:1130	arg1	morphology					1132:1141	Surface morphology	1124:1141	Surface morphology by field emission scanning electron microscopy	1124:1188	Surface morphology by field emission scanning electron microscopy reveals the presence of protrusions in fiber which aid in the better adhesion with the matrix in composite manufacturing.
32536404	3	42	theme	Kigelia	696:702	arg1	fiber					713:717	the Kigelia africana fiber	692:717	the Kigelia africana fiber	692:717	The physical analysis revealed the mean tensile strength as 50.31 ± 24.71 to 73.12 ± 32.48 MPa, diameter as 0.507 ± 0.162 to 0.629 ± 0.182 mm and density as 1.316 g/cm³ for the Kigelia africana fiber.
32536404	4	43	dep	Fourier	906:912	arg1	transform					914:922	transform	914:922	transform infrared spectroscopy analysis	914:953	The proximate chemical analysis estimated the cellulose percentage to be 61.5 % and the existence of different basic components like cellulose, hemicellulose and lignin are confirmed by Fourier transform infrared spectroscopy analysis.
32536404	7	44	theme	electron	1170:1177	arg1	microscopy					1179:1188	field emission scanning electron microscopy	1146:1188	field emission scanning electron microscopy	1146:1188	Surface morphology by field emission scanning electron microscopy reveals the presence of protrusions in fiber which aid in the better adhesion with the matrix in composite manufacturing.
32536404	2	45	theme	cellulose	294:302	arg1	fiber					304:308	a novel cellulose fiber	286:308	a novel cellulose fiber	286:308	In line with this, a novel cellulose fiber was extracted from the Kigelia africana fruit and its physical, chemical and thermal properties, crystallography and surface morphology analysis were studied and reported in this investigative research paper.
32536404	3	46	theme	±	633:633	arg1	±					650:650	0.507 ± 0.162 to 0.629 ±	627:650	0.507 ± 0.162 to 0.629 ±	627:650	The physical analysis revealed the mean tensile strength as 50.31 ± 24.71 to 73.12 ± 32.48 MPa, diameter as 0.507 ± 0.162 to 0.629 ± 0.182 mm and density as 1.316 g/cm³ for the Kigelia africana fiber.
32536404	7	47	with	adhesion	1259:1266	arg1	matrix					1277:1282	the matrix	1273:1282	the matrix	1273:1282	Surface morphology by field emission scanning electron microscopy reveals the presence of protrusions in fiber which aid in the better adhesion with the matrix in composite manufacturing.
32536404	3	48	theme	mean	554:557	arg1	strength					567:574	the mean tensile strength	550:574	the mean tensile strength	550:574	The physical analysis revealed the mean tensile strength as 50.31 ± 24.71 to 73.12 ± 32.48 MPa, diameter as 0.507 ± 0.162 to 0.629 ± 0.182 mm and density as 1.316 g/cm³ for the Kigelia africana fiber.
32536404	1	49	theme	recent	105:110	arg1	days					112:115	recent days	105:115	recent days	105:115	In recent days, there is an increasing use of green composites in composite manufacturing, where cellulosic natural fibers have been started using for this purpose.
32536404	4	50	theme	different	821:829	arg1	components					837:846	different basic components	821:846	different basic components	821:846	The proximate chemical analysis estimated the cellulose percentage to be 61.5 % and the existence of different basic components like cellulose, hemicellulose and lignin are confirmed by Fourier transform infrared spectroscopy analysis.
32536404	5	51	theme	thermal	999:1005	arg1	stability					1007:1015	the thermal stability	995:1015	the thermal stability of the fiber	995:1028	Thermogravimetric analysis establishes the thermal stability of the fiber as 212 ⁰C.
32536404	2	52	dep	Kigelia	333:339	arg1	africana					341:348	africana	341:348	africana	341:348	In line with this, a novel cellulose fiber was extracted from the Kigelia africana fruit and its physical, chemical and thermal properties, crystallography and surface morphology analysis were studied and reported in this investigative research paper.
32536404	4	53	theme	proximate	724:732	arg1	analysis					743:750	The proximate chemical analysis	720:750	The proximate chemical analysis	720:750	The proximate chemical analysis estimated the cellulose percentage to be 61.5 % and the existence of different basic components like cellulose, hemicellulose and lignin are confirmed by Fourier transform infrared spectroscopy analysis.
32536404	7	54	theme	composite	1287:1295	arg1	manufacturing					1297:1309	composite manufacturing	1287:1309	composite manufacturing	1287:1309	Surface morphology by field emission scanning electron microscopy reveals the presence of protrusions in fiber which aid in the better adhesion with the matrix in composite manufacturing.
32536404	5	55	theme	fiber	1024:1028	arg1	stability					1007:1015	the thermal stability	995:1015	the thermal stability of the fiber	995:1028	Thermogravimetric analysis establishes the thermal stability of the fiber as 212 ⁰C.
32536404	7	56	theme	protrusions	1214:1224	arg1	presence					1202:1209	the presence	1198:1209	the presence of protrusions in fiber which aid in the better adhesion with the matrix in composite manufacturing	1198:1309	Surface morphology by field emission scanning electron microscopy reveals the presence of protrusions in fiber which aid in the better adhesion with the matrix in composite manufacturing.
32536404	7	57	theme	better	1252:1257	arg1	adhesion					1259:1266	the better adhesion	1248:1266	the better adhesion with the matrix in composite manufacturing	1248:1309	Surface morphology by field emission scanning electron microscopy reveals the presence of protrusions in fiber which aid in the better adhesion with the matrix in composite manufacturing.
32536404	1	58	theme	cellulosic	199:208	arg1	fibers					218:223	cellulosic natural fibers	199:223	cellulosic natural fibers	199:223	In recent days, there is an increasing use of green composites in composite manufacturing, where cellulosic natural fibers have been started using for this purpose.
32536404	0	59	theme	africana	55:62	arg1	fruit					64:68	Kigelia africana fruit	47:68	Kigelia africana fruit	47:68	Study on a Novel natural cellulosic fiber from Kigelia africana fruit: Characterization and analysis.
32536404	7	60	from	adhesion	1259:1266	arg1	manufacturing					1297:1309	composite manufacturing	1287:1309	composite manufacturing	1287:1309	Surface morphology by field emission scanning electron microscopy reveals the presence of protrusions in fiber which aid in the better adhesion with the matrix in composite manufacturing.
32536404	6	61	theme	crystallinity	1045:1057	arg1	index					1059:1063	The crystallinity index	1041:1063	The crystallinity index	1041:1063	The crystallinity index, 57.38 % of the fiber was determined by X-ray diffraction.
32536404	6	61	theme	crystallinity	1045:1057	arg1	%					1072:1072	57.38 %	1066:1072	57.38 %	1066:1072	The crystallinity index, 57.38 % of the fiber was determined by X-ray diffraction.
32536404	1	62	theme	natural	210:216	arg1	fibers					218:223	cellulosic natural fibers	199:223	cellulosic natural fibers	199:223	In recent days, there is an increasing use of green composites in composite manufacturing, where cellulosic natural fibers have been started using for this purpose.
32536404	0	63	theme	Kigelia	47:53	arg1	fruit					64:68	Kigelia africana fruit	47:68	Kigelia africana fruit	47:68	Study on a Novel natural cellulosic fiber from Kigelia africana fruit: Characterization and analysis.
32536404	7	64	attach	presence	1202:1209	arg2	protrusions					1214:1224	protrusions	1214:1224	protrusions	1214:1224	Surface morphology by field emission scanning electron microscopy reveals the presence of protrusions in fiber which aid in the better adhesion with the matrix in composite manufacturing.
32536404	7	64	attach	presence	1202:1209	arg1	fiber					1229:1233	fiber	1229:1233	fiber	1229:1233	Surface morphology by field emission scanning electron microscopy reveals the presence of protrusions in fiber which aid in the better adhesion with the matrix in composite manufacturing.
32536404	0	65	dep	Study	0:4	arg1	analysis					92:99	analysis	92:99	analysis	92:99	Study on a Novel natural cellulosic fiber from Kigelia africana fruit: Characterization and analysis.
32536404	0	65	dep	Study	0:4	arg1	Characterization					71:86	Characterization	71:86	Characterization	71:86	Study on a Novel natural cellulosic fiber from Kigelia africana fruit: Characterization and analysis.
32536404	1	66	from	use	141:143	arg1	manufacturing					178:190	composite manufacturing	168:190	composite manufacturing	168:190	In recent days, there is an increasing use of green composites in composite manufacturing, where cellulosic natural fibers have been started using for this purpose.
34766958	5	0	theme	cellulose	933:941	arg1	fibers					943:948	cellulose fibers	933:948	cellulose fibers	933:948	These results are significant as they indicate the possibility to monitor and control fiber dosing and subsequent dope viscosity during chemical recycling of cellulose fibers.
34766958	4	1	theme	consumer	534:541	arg1	textiles					543:550	consumer textiles	534:550	consumer textiles	534:550	Our classifiers trained on images of consumer textiles showed 100% true positive rates based on model cross-validation and correctly identified on average 8-9 out of 10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions.
34766958	4	2	theme	lyocell	736:742	arg1	samples					744:750	specifically made cotton, viscose and lyocell samples	698:750	specifically made cotton, viscose and lyocell samples of known compositions	698:772	Our classifiers trained on images of consumer textiles showed 100% true positive rates based on model cross-validation and correctly identified on average 8-9 out of 10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions.
34766958	0	3	theme	cellulose	18:26	arg1	fibers					36:41	cellulose textile fibers	18:41	cellulose textile fibers	18:41	Identification of cellulose textile fibers.
34766958	2	4	theme	Most	127:130	arg1	studies					132:138	Most studies	127:138	Most studies on non-destructive optical textile identification	127:188	Most studies on non-destructive optical textile identification have focused on classifying different synthetic and natural fibers but chemical recycling requires more detailed information on fiber composition and polymer properties.
34766958	3	5	theme	near	387:390	arg1	spectroscopy					409:420	near infrared imaging spectroscopy	387:420	near infrared imaging spectroscopy	387:420	Here, we report the use of near infrared imaging spectroscopy and chemometrics for classifying natural and regenerated cellulose fibers.
34766958	4	6	theme	%	562:562	arg1	rates					578:582	100% true positive rates	559:582	100% true positive rates	559:582	Our classifiers trained on images of consumer textiles showed 100% true positive rates based on model cross-validation and correctly identified on average 8-9 out of 10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions.
34766958	7	7	theme	imaging	1174:1180	arg1	spectroscopy					1182:1193	imaging spectroscopy	1174:1193	imaging spectroscopy	1174:1193	This finding opens the possibility to indirectly estimate dope viscosity and creates entirely new hypotheses for combining imaging spectroscopy with classification and regression methods within the broader field of cellulose modification.
34766958	1	8	theme	textile	69:75	arg1	fibers					77:82	different textile fibers	59:82	different textile fibers	59:82	Distinguishing different textile fibers is important for recycling waste textiles.
34766958	3	9	theme	infrared	392:399	arg1	spectroscopy					409:420	near infrared imaging spectroscopy	387:420	near infrared imaging spectroscopy	387:420	Here, we report the use of near infrared imaging spectroscopy and chemometrics for classifying natural and regenerated cellulose fibers.
34766958	6	10	theme	chain	1037:1041	arg1	length					1043:1048	polymer chain length	1029:1048	polymer chain length	1029:1048	Our results also suggested the possibility to identify fibers purely based on polymer chain length.
34766958	7	11	theme	cellulose	1266:1274	arg1	modification					1276:1287	cellulose modification	1266:1287	cellulose modification	1266:1287	This finding opens the possibility to indirectly estimate dope viscosity and creates entirely new hypotheses for combining imaging spectroscopy with classification and regression methods within the broader field of cellulose modification.
34766958	2	12	theme	textile	167:173	arg1	identification					175:188	non-destructive optical textile identification	143:188	non-destructive optical textile identification	143:188	Most studies on non-destructive optical textile identification have focused on classifying different synthetic and natural fibers but chemical recycling requires more detailed information on fiber composition and polymer properties.
34766958	7	13	theme	broader	1249:1255	arg1	field					1257:1261	the broader field	1245:1261	the broader field of cellulose modification	1245:1287	This finding opens the possibility to indirectly estimate dope viscosity and creates entirely new hypotheses for combining imaging spectroscopy with classification and regression methods within the broader field of cellulose modification.
34766958	5	14	theme	subsequent	878:887	arg1	viscosity					894:902	subsequent dope viscosity	878:902	subsequent dope viscosity	878:902	These results are significant as they indicate the possibility to monitor and control fiber dosing and subsequent dope viscosity during chemical recycling of cellulose fibers.
34766958	2	15	theme	fiber	318:322	arg1	composition					324:334	fiber composition	318:334	fiber composition	318:334	Most studies on non-destructive optical textile identification have focused on classifying different synthetic and natural fibers but chemical recycling requires more detailed information on fiber composition and polymer properties.
34766958	4	16	theme	cotton	716:721	arg1	samples					744:750	specifically made cotton, viscose and lyocell samples	698:750	specifically made cotton, viscose and lyocell samples of known compositions	698:772	Our classifiers trained on images of consumer textiles showed 100% true positive rates based on model cross-validation and correctly identified on average 8-9 out of 10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions.
34766958	7	17	theme	modification	1276:1287	arg1	field					1257:1261	the broader field	1245:1261	the broader field of cellulose modification	1245:1287	This finding opens the possibility to indirectly estimate dope viscosity and creates entirely new hypotheses for combining imaging spectroscopy with classification and regression methods within the broader field of cellulose modification.
34766958	2	18	theme	optical	159:165	arg1	identification					175:188	non-destructive optical textile identification	143:188	non-destructive optical textile identification	143:188	Most studies on non-destructive optical textile identification have focused on classifying different synthetic and natural fibers but chemical recycling requires more detailed information on fiber composition and polymer properties.
34766958	5	19	theme	control	853:859	arg1	dosing					867:872	control fiber dosing	853:872	control fiber dosing	853:872	These results are significant as they indicate the possibility to monitor and control fiber dosing and subsequent dope viscosity during chemical recycling of cellulose fibers.
34766958	5	20	theme	chemical	911:918	arg1	recycling					920:928	chemical recycling	911:928	chemical recycling of cellulose fibers	911:948	These results are significant as they indicate the possibility to monitor and control fiber dosing and subsequent dope viscosity during chemical recycling of cellulose fibers.
34766958	2	21	theme	non-destructive	143:157	arg1	identification					175:188	non-destructive optical textile identification	143:188	non-destructive optical textile identification	143:188	Most studies on non-destructive optical textile identification have focused on classifying different synthetic and natural fibers but chemical recycling requires more detailed information on fiber composition and polymer properties.
34766958	7	22	theme	new	1145:1147	arg1	hypotheses					1149:1158	entirely new hypotheses	1136:1158	entirely new hypotheses for combining imaging spectroscopy with classification and regression methods within the broader field of cellulose modification	1136:1287	This finding opens the possibility to indirectly estimate dope viscosity and creates entirely new hypotheses for combining imaging spectroscopy with classification and regression methods within the broader field of cellulose modification.
34766958	3	23	theme	regenerated	467:477	arg1	fibers					489:494	natural and regenerated cellulose fibers	455:494	natural and regenerated cellulose fibers	455:494	Here, we report the use of near infrared imaging spectroscopy and chemometrics for classifying natural and regenerated cellulose fibers.
34766958	4	24	theme	true	564:567	arg1	rates					578:582	100% true positive rates	559:582	100% true positive rates	559:582	Our classifiers trained on images of consumer textiles showed 100% true positive rates based on model cross-validation and correctly identified on average 8-9 out of 10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions.
34766958	6	25	theme	polymer	1029:1035	arg1	length					1043:1048	polymer chain length	1029:1048	polymer chain length	1029:1048	Our results also suggested the possibility to identify fibers purely based on polymer chain length.
34766958	0	26	theme	textile	28:34	arg1	fibers					36:41	cellulose textile fibers	18:41	cellulose textile fibers	18:41	Identification of cellulose textile fibers.
34766958	3	27	theme	cellulose	479:487	arg1	fibers					489:494	natural and regenerated cellulose fibers	455:494	natural and regenerated cellulose fibers	455:494	Here, we report the use of near infrared imaging spectroscopy and chemometrics for classifying natural and regenerated cellulose fibers.
34766958	3	28	theme	imaging	401:407	arg1	spectroscopy					409:420	near infrared imaging spectroscopy	387:420	near infrared imaging spectroscopy	387:420	Here, we report the use of near infrared imaging spectroscopy and chemometrics for classifying natural and regenerated cellulose fibers.
34766958	4	29	theme	model	593:597	arg1	cross-validation					599:614	model cross-validation	593:614	model cross-validation	593:614	Our classifiers trained on images of consumer textiles showed 100% true positive rates based on model cross-validation and correctly identified on average 8-9 out of 10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions.
34766958	1	30	theme	waste	111:115	arg1	textiles					117:124	waste textiles	111:124	waste textiles	111:124	Distinguishing different textile fibers is important for recycling waste textiles.
34766958	4	31	theme	pixels	675:680	arg1	average					644:650	average 8-9	644:654	average 8-9 out of 10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions	644:772	Our classifiers trained on images of consumer textiles showed 100% true positive rates based on model cross-validation and correctly identified on average 8-9 out of 10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions.
34766958	2	32	from	studies	132:138	arg1	identification					175:188	non-destructive optical textile identification	143:188	non-destructive optical textile identification	143:188	Most studies on non-destructive optical textile identification have focused on classifying different synthetic and natural fibers but chemical recycling requires more detailed information on fiber composition and polymer properties.
34766958	2	33	theme	polymer	340:346	arg1	properties					348:357	polymer properties	340:357	polymer properties	340:357	Most studies on non-destructive optical textile identification have focused on classifying different synthetic and natural fibers but chemical recycling requires more detailed information on fiber composition and polymer properties.
34766958	2	34	from	information	303:313	arg1	composition					324:334	fiber composition	318:334	fiber composition	318:334	Most studies on non-destructive optical textile identification have focused on classifying different synthetic and natural fibers but chemical recycling requires more detailed information on fiber composition and polymer properties.
34766958	2	34	from	information	303:313	arg1	properties					348:357	polymer properties	340:357	polymer properties	340:357	Most studies on non-destructive optical textile identification have focused on classifying different synthetic and natural fibers but chemical recycling requires more detailed information on fiber composition and polymer properties.
34766958	4	35	theme	set	671:673	arg1	pixels					675:680	10 test set pixels	663:680	10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions	663:772	Our classifiers trained on images of consumer textiles showed 100% true positive rates based on model cross-validation and correctly identified on average 8-9 out of 10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions.
34766958	3	36	theme	natural	455:461	arg1	fibers					489:494	natural and regenerated cellulose fibers	455:494	natural and regenerated cellulose fibers	455:494	Here, we report the use of near infrared imaging spectroscopy and chemometrics for classifying natural and regenerated cellulose fibers.
34766958	4	37	theme	made	711:714	arg1	cotton					716:721	specifically made cotton	698:721	specifically made cotton	698:721	Our classifiers trained on images of consumer textiles showed 100% true positive rates based on model cross-validation and correctly identified on average 8-9 out of 10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions.
34766958	5	38	theme	dope	889:892	arg1	viscosity					894:902	subsequent dope viscosity	878:902	subsequent dope viscosity	878:902	These results are significant as they indicate the possibility to monitor and control fiber dosing and subsequent dope viscosity during chemical recycling of cellulose fibers.
34766958	0	39	theme	fibers	36:41	arg1	Identification					0:13	Identification	0:13	Identification of cellulose textile fibers.	0:42	Identification of cellulose textile fibers.
34766958	2	40	theme	chemical	261:268	arg1	recycling					270:278	chemical recycling	261:278	chemical recycling	261:278	Most studies on non-destructive optical textile identification have focused on classifying different synthetic and natural fibers but chemical recycling requires more detailed information on fiber composition and polymer properties.
34766958	5	41	theme	fiber	861:865	arg1	dosing					867:872	control fiber dosing	853:872	control fiber dosing	853:872	These results are significant as they indicate the possibility to monitor and control fiber dosing and subsequent dope viscosity during chemical recycling of cellulose fibers.
34766958	4	42	theme	positive	569:576	arg1	rates					578:582	100% true positive rates	559:582	100% true positive rates	559:582	Our classifiers trained on images of consumer textiles showed 100% true positive rates based on model cross-validation and correctly identified on average 8-9 out of 10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions.
34766958	4	43	dep	pixels	675:680	arg1	out					656:658	out	656:658	out	656:658	Our classifiers trained on images of consumer textiles showed 100% true positive rates based on model cross-validation and correctly identified on average 8-9 out of 10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions.
34766958	4	44	theme	textiles	543:550	arg1	images					524:529	images	524:529	images of consumer textiles	524:550	Our classifiers trained on images of consumer textiles showed 100% true positive rates based on model cross-validation and correctly identified on average 8-9 out of 10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions.
34766958	7	45	theme	regression	1219:1228	arg1	methods					1230:1236	regression methods	1219:1236	regression methods	1219:1236	This finding opens the possibility to indirectly estimate dope viscosity and creates entirely new hypotheses for combining imaging spectroscopy with classification and regression methods within the broader field of cellulose modification.
34766958	7	46	theme	dope	1109:1112	arg1	viscosity					1114:1122	dope viscosity	1109:1122	dope viscosity	1109:1122	This finding opens the possibility to indirectly estimate dope viscosity and creates entirely new hypotheses for combining imaging spectroscopy with classification and regression methods within the broader field of cellulose modification.
34766958	2	47	theme	natural	242:248	arg1	fibers					250:255	different synthetic and natural fibers	218:255	different synthetic and natural fibers	218:255	Most studies on non-destructive optical textile identification have focused on classifying different synthetic and natural fibers but chemical recycling requires more detailed information on fiber composition and polymer properties.
34766958	4	48	theme	samples	744:750	arg1	images					688:693	images	688:693	images of specifically made cotton, viscose and lyocell samples of known compositions	688:772	Our classifiers trained on images of consumer textiles showed 100% true positive rates based on model cross-validation and correctly identified on average 8-9 out of 10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions.
34766958	1	49	theme	different	59:67	arg1	fibers					77:82	different textile fibers	59:82	different textile fibers	59:82	Distinguishing different textile fibers is important for recycling waste textiles.
34766958	4	50	theme	compositions	761:772	arg1	samples					744:750	specifically made cotton, viscose and lyocell samples	698:750	specifically made cotton, viscose and lyocell samples of known compositions	698:772	Our classifiers trained on images of consumer textiles showed 100% true positive rates based on model cross-validation and correctly identified on average 8-9 out of 10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions.
34766958	2	51	theme	synthetic	228:236	arg1	fibers					250:255	different synthetic and natural fibers	218:255	different synthetic and natural fibers	218:255	Most studies on non-destructive optical textile identification have focused on classifying different synthetic and natural fibers but chemical recycling requires more detailed information on fiber composition and polymer properties.
34766958	2	52	theme	detailed	294:301	arg1	information					303:313	more detailed information	289:313	more detailed information on fiber composition and polymer properties	289:357	Most studies on non-destructive optical textile identification have focused on classifying different synthetic and natural fibers but chemical recycling requires more detailed information on fiber composition and polymer properties.
34766958	4	53	theme	known	755:759	arg1	compositions					761:772	known compositions	755:772	known compositions	755:772	Our classifiers trained on images of consumer textiles showed 100% true positive rates based on model cross-validation and correctly identified on average 8-9 out of 10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions.
34766958	2	54	theme	different	218:226	arg1	fibers					250:255	different synthetic and natural fibers	218:255	different synthetic and natural fibers	218:255	Most studies on non-destructive optical textile identification have focused on classifying different synthetic and natural fibers but chemical recycling requires more detailed information on fiber composition and polymer properties.
34766958	4	55	theme	viscose	724:730	arg1	samples					744:750	specifically made cotton, viscose and lyocell samples	698:750	specifically made cotton, viscose and lyocell samples of known compositions	698:772	Our classifiers trained on images of consumer textiles showed 100% true positive rates based on model cross-validation and correctly identified on average 8-9 out of 10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions.
34766958	4	56	theme	test	666:669	arg1	pixels					675:680	10 test set pixels	663:680	10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions	663:772	Our classifiers trained on images of consumer textiles showed 100% true positive rates based on model cross-validation and correctly identified on average 8-9 out of 10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions.
34766958	4	57	theme	100	559:561	arg1	%					562:562	%	562:562	%	562:562	Our classifiers trained on images of consumer textiles showed 100% true positive rates based on model cross-validation and correctly identified on average 8-9 out of 10 test set pixels using images of specifically made cotton, viscose and lyocell samples of known compositions.
34766958	3	58	theme	spectroscopy	409:420	arg1	chemometrics					426:437	chemometrics	426:437	chemometrics for classifying natural and regenerated cellulose fibers	426:494	Here, we report the use of near infrared imaging spectroscopy and chemometrics for classifying natural and regenerated cellulose fibers.
34766958	3	58	theme	spectroscopy	409:420	arg1	use					380:382	the use	376:382	the use of near infrared imaging spectroscopy	376:420	Here, we report the use of near infrared imaging spectroscopy and chemometrics for classifying natural and regenerated cellulose fibers.
34766958	5	59	theme	fibers	943:948	arg1	recycling					920:928	chemical recycling	911:928	chemical recycling of cellulose fibers	911:948	These results are significant as they indicate the possibility to monitor and control fiber dosing and subsequent dope viscosity during chemical recycling of cellulose fibers.
34260208	0	0	theme	Oligosaccharide	83:97	arg1	Method					120:125	an In Situ Oligosaccharide Surface Modification Method	72:125	an In Situ Oligosaccharide Surface Modification Method	72:125	Tuning the Physicochemical Properties of Cellulose Nanocrystals through an In Situ Oligosaccharide Surface Modification Method.
34260208	7	1	theme	expanded	1317:1324	arg1	functionality					1330:1342	expanded CNC functionality	1317:1342	expanded CNC functionality	1317:1342	Overall, this fundamental study introduces an easily scalable modification route that opens the door for expanded CNC functionality and applications.
34260208	3	2	theme	surface	540:546	arg1	method					561:566	a novel surface modification method	532:566	a novel surface modification method with the potential to tune CNC properties through the in situ deposition of cellulose phosphate oligosaccharides during CNC production	532:701	Presented here is a novel surface modification method with the potential to tune CNC properties through the in situ deposition of cellulose phosphate oligosaccharides during CNC production.
34260208	7	3	theme	CNC	1326:1328	arg1	functionality					1330:1342	expanded CNC functionality	1317:1342	expanded CNC functionality	1317:1342	Overall, this fundamental study introduces an easily scalable modification route that opens the door for expanded CNC functionality and applications.
34260208	7	4	theme	fundamental	1226:1236	arg1	study					1238:1242	this fundamental study	1221:1242	this fundamental study	1221:1242	Overall, this fundamental study introduces an easily scalable modification route that opens the door for expanded CNC functionality and applications.
34260208	6	5	theme	polymerization	1196:1209	arg1	degree					1186:1191	the oligosaccharide degree	1166:1191	the oligosaccharide degree of polymerization	1166:1209	CNC surface coverage was tuned by controlling the oligosaccharide degree of polymerization.
34260208	1	6	theme	sustainable	179:189	arg1	alternatives					191:202	sustainable alternatives	179:202	sustainable alternatives	179:202	The trend to replace petroleum-based products with sustainable alternatives has shifted research efforts toward plant-based materials such as cellulose nanocrystals (CNCs).
34260208	0	7	theme	Modification	107:118	arg1	Method					120:125	an In Situ Oligosaccharide Surface Modification Method	72:125	an In Situ Oligosaccharide Surface Modification Method	72:125	Tuning the Physicochemical Properties of Cellulose Nanocrystals through an In Situ Oligosaccharide Surface Modification Method.
34260208	3	8	with	method	561:566	arg1	potential					577:585	the potential to tune CNC properties through the in situ deposition of cellulose phosphate oligosaccharides during CNC production	573:701	the potential to tune CNC properties through the in situ deposition of cellulose phosphate oligosaccharides during CNC production	573:701	Presented here is a novel surface modification method with the potential to tune CNC properties through the in situ deposition of cellulose phosphate oligosaccharides during CNC production.
34260208	5	9	theme	lower	1034:1038	arg1	capacities					1057:1066	lower water adsorption capacities	1034:1066	lower water adsorption capacities	1034:1066	Oligosaccharide-coated CNCs demonstrated subtle changes including higher surface charge densities and lower water adsorption capacities and viscosities than their unmodified counterparts.
34260208	0	10	theme	Surface	99:105	arg1	Method					120:125	an In Situ Oligosaccharide Surface Modification Method	72:125	an In Situ Oligosaccharide Surface Modification Method	72:125	Tuning the Physicochemical Properties of Cellulose Nanocrystals through an In Situ Oligosaccharide Surface Modification Method.
34260208	5	11	theme	water	1040:1044	arg1	capacities					1057:1066	lower water adsorption capacities	1034:1066	lower water adsorption capacities	1034:1066	Oligosaccharide-coated CNCs demonstrated subtle changes including higher surface charge densities and lower water adsorption capacities and viscosities than their unmodified counterparts.
34260208	2	12	dep	applications	331:342	arg1	composites					351:360	composites	351:360	composites	351:360	CNCs show promise in numerous applications (e.g., composites and rheological modifiers); however, maximizing their performance often requires surface modifications with complex chemistries and purification steps.
34260208	2	12	dep	applications	331:342	arg1	modifiers					378:386	rheological modifiers	366:386	rheological modifiers	366:386	CNCs show promise in numerous applications (e.g., composites and rheological modifiers); however, maximizing their performance often requires surface modifications with complex chemistries and purification steps.
34260208	4	13	theme	CNC	882:884	arg1	surfaces					886:893	CNC surfaces	882:893	CNC surfaces	882:893	This was achieved by leveraging the selective solubility of oligosaccharides, which are soluble at a low pH (during the CNC hydrolysis) yet become insoluble and precipitate onto CNC surfaces upon increasing pH during quenching.
34260208	4	14	from	pH	809:810	arg1	soluble					792:798	soluble	792:798	soluble	792:798	This was achieved by leveraging the selective solubility of oligosaccharides, which are soluble at a low pH (during the CNC hydrolysis) yet become insoluble and precipitate onto CNC surfaces upon increasing pH during quenching.
34260208	5	15	theme	adsorption	1046:1055	arg1	capacities					1057:1066	lower water adsorption capacities	1034:1066	lower water adsorption capacities	1034:1066	Oligosaccharide-coated CNCs demonstrated subtle changes including higher surface charge densities and lower water adsorption capacities and viscosities than their unmodified counterparts.
34260208	3	16	dep	in	622:623	arg1	situ					625:628	situ	625:628	situ	625:628	Presented here is a novel surface modification method with the potential to tune CNC properties through the in situ deposition of cellulose phosphate oligosaccharides during CNC production.
34260208	0	17	theme	Nanocrystals	51:62	arg1	Properties					27:36	the Physicochemical Properties	7:36	the Physicochemical Properties of Cellulose Nanocrystals	7:62	Tuning the Physicochemical Properties of Cellulose Nanocrystals through an In Situ Oligosaccharide Surface Modification Method.
34260208	2	18	with	modifications	451:463	arg1	chemistries					478:488	complex chemistries	470:488	complex chemistries	470:488	CNCs show promise in numerous applications (e.g., composites and rheological modifiers); however, maximizing their performance often requires surface modifications with complex chemistries and purification steps.
34260208	2	18	with	modifications	451:463	arg1	steps					507:511	purification steps	494:511	purification steps	494:511	CNCs show promise in numerous applications (e.g., composites and rheological modifiers); however, maximizing their performance often requires surface modifications with complex chemistries and purification steps.
34260208	5	19	theme	surface	1005:1011	arg1	densities					1020:1028	higher surface charge densities	998:1028	higher surface charge densities	998:1028	Oligosaccharide-coated CNCs demonstrated subtle changes including higher surface charge densities and lower water adsorption capacities and viscosities than their unmodified counterparts.
34260208	6	20	theme	oligosaccharide	1170:1184	arg1	degree					1186:1191	the oligosaccharide degree	1166:1191	the oligosaccharide degree of polymerization	1166:1209	CNC surface coverage was tuned by controlling the oligosaccharide degree of polymerization.
34260208	2	21	dep	composites	351:360	arg1	e.g.					345:348	e.g.	345:348	e.g.	345:348	CNCs show promise in numerous applications (e.g., composites and rheological modifiers); however, maximizing their performance often requires surface modifications with complex chemistries and purification steps.
34260208	5	22	theme	charge	1013:1018	arg1	densities					1020:1028	higher surface charge densities	998:1028	higher surface charge densities	998:1028	Oligosaccharide-coated CNCs demonstrated subtle changes including higher surface charge densities and lower water adsorption capacities and viscosities than their unmodified counterparts.
34260208	1	23	theme	research	216:223	arg1	efforts					225:231	research efforts	216:231	research efforts	216:231	The trend to replace petroleum-based products with sustainable alternatives has shifted research efforts toward plant-based materials such as cellulose nanocrystals (CNCs).
34260208	2	24	theme	complex	470:476	arg1	chemistries					478:488	complex chemistries	470:488	complex chemistries	470:488	CNCs show promise in numerous applications (e.g., composites and rheological modifiers); however, maximizing their performance often requires surface modifications with complex chemistries and purification steps.
34260208	0	25	dep	In	75:76	arg1	Situ					78:81	Situ	78:81	Situ	78:81	Tuning the Physicochemical Properties of Cellulose Nanocrystals through an In Situ Oligosaccharide Surface Modification Method.
34260208	0	26	theme	Physicochemical	11:25	arg1	Properties					27:36	the Physicochemical Properties	7:36	the Physicochemical Properties of Cellulose Nanocrystals	7:62	Tuning the Physicochemical Properties of Cellulose Nanocrystals through an In Situ Oligosaccharide Surface Modification Method.
34260208	2	27	theme	numerous	322:329	arg1	applications					331:342	numerous applications	322:342	numerous applications (e.g., composites and rheological modifiers)	322:387	CNCs show promise in numerous applications (e.g., composites and rheological modifiers); however, maximizing their performance often requires surface modifications with complex chemistries and purification steps.
34260208	2	28	theme	surface	443:449	arg1	modifications					451:463	surface modifications	443:463	surface modifications with complex chemistries and purification steps	443:511	CNCs show promise in numerous applications (e.g., composites and rheological modifiers); however, maximizing their performance often requires surface modifications with complex chemistries and purification steps.
34260208	0	29	theme	Cellulose	41:49	arg1	Nanocrystals					51:62	Cellulose Nanocrystals	41:62	Cellulose Nanocrystals	41:62	Tuning the Physicochemical Properties of Cellulose Nanocrystals through an In Situ Oligosaccharide Surface Modification Method.
34260208	4	30	theme	oligosaccharides	764:779	arg1	solubility					750:759	the selective solubility	736:759	the selective solubility of oligosaccharides, which are soluble at a low pH (during the CNC hydrolysis) yet become insoluble and precipitate onto CNC surfaces upon increasing pH during quenching	736:929	This was achieved by leveraging the selective solubility of oligosaccharides, which are soluble at a low pH (during the CNC hydrolysis) yet become insoluble and precipitate onto CNC surfaces upon increasing pH during quenching.
34260208	5	31	theme	unmodified	1095:1104	arg1	counterparts					1106:1117	their unmodified counterparts	1089:1117	their unmodified counterparts	1089:1117	Oligosaccharide-coated CNCs demonstrated subtle changes including higher surface charge densities and lower water adsorption capacities and viscosities than their unmodified counterparts.
34260208	1	32	theme	plant-based	240:250	arg1	nanocrystals					280:291	cellulose nanocrystals	270:291	cellulose nanocrystals (CNCs)	270:298	The trend to replace petroleum-based products with sustainable alternatives has shifted research efforts toward plant-based materials such as cellulose nanocrystals (CNCs).
34260208	1	32	theme	plant-based	240:250	arg1	materials					252:260	plant-based materials	240:260	plant-based materials such as cellulose nanocrystals (CNCs)	240:298	The trend to replace petroleum-based products with sustainable alternatives has shifted research efforts toward plant-based materials such as cellulose nanocrystals (CNCs).
34260208	4	33	theme	selective	740:748	arg1	solubility					750:759	the selective solubility	736:759	the selective solubility of oligosaccharides, which are soluble at a low pH (during the CNC hydrolysis) yet become insoluble and precipitate onto CNC surfaces upon increasing pH during quenching	736:929	This was achieved by leveraging the selective solubility of oligosaccharides, which are soluble at a low pH (during the CNC hydrolysis) yet become insoluble and precipitate onto CNC surfaces upon increasing pH during quenching.
34260208	7	34	theme	modification	1274:1285	arg1	route					1287:1291	an easily scalable modification route	1255:1291	an easily scalable modification route that opens the door for expanded CNC functionality and applications	1255:1359	Overall, this fundamental study introduces an easily scalable modification route that opens the door for expanded CNC functionality and applications.
34260208	3	35	theme	CNC	688:690	arg1	production					692:701	CNC production	688:701	CNC production	688:701	Presented here is a novel surface modification method with the potential to tune CNC properties through the in situ deposition of cellulose phosphate oligosaccharides during CNC production.
34260208	7	36	theme	scalable	1265:1272	arg1	route					1287:1291	an easily scalable modification route	1255:1291	an easily scalable modification route that opens the door for expanded CNC functionality and applications	1255:1359	Overall, this fundamental study introduces an easily scalable modification route that opens the door for expanded CNC functionality and applications.
34260208	4	37	theme	low	805:807	arg1	pH					809:810	a low pH	803:810	a low pH (during the CNC hydrolysis)	803:838	This was achieved by leveraging the selective solubility of oligosaccharides, which are soluble at a low pH (during the CNC hydrolysis) yet become insoluble and precipitate onto CNC surfaces upon increasing pH during quenching.
34260208	3	38	theme	in	622:623	arg1	deposition					630:639	the in situ deposition	618:639	the in situ deposition of cellulose phosphate oligosaccharides during CNC production	618:701	Presented here is a novel surface modification method with the potential to tune CNC properties through the in situ deposition of cellulose phosphate oligosaccharides during CNC production.
34260208	2	39	theme	rheological	366:376	arg1	modifiers					378:386	rheological modifiers	366:386	rheological modifiers	366:386	CNCs show promise in numerous applications (e.g., composites and rheological modifiers); however, maximizing their performance often requires surface modifications with complex chemistries and purification steps.
34260208	2	40	theme	purification	494:505	arg1	steps					507:511	purification steps	494:511	purification steps	494:511	CNCs show promise in numerous applications (e.g., composites and rheological modifiers); however, maximizing their performance often requires surface modifications with complex chemistries and purification steps.
34260208	3	41	theme	modification	548:559	arg1	method					561:566	a novel surface modification method	532:566	a novel surface modification method with the potential to tune CNC properties through the in situ deposition of cellulose phosphate oligosaccharides during CNC production	532:701	Presented here is a novel surface modification method with the potential to tune CNC properties through the in situ deposition of cellulose phosphate oligosaccharides during CNC production.
34260208	3	42	theme	novel	534:538	arg1	method					561:566	a novel surface modification method	532:566	a novel surface modification method with the potential to tune CNC properties through the in situ deposition of cellulose phosphate oligosaccharides during CNC production	532:701	Presented here is a novel surface modification method with the potential to tune CNC properties through the in situ deposition of cellulose phosphate oligosaccharides during CNC production.
34260208	3	43	theme	oligosaccharides	664:679	arg1	deposition					630:639	the in situ deposition	618:639	the in situ deposition of cellulose phosphate oligosaccharides during CNC production	618:701	Presented here is a novel surface modification method with the potential to tune CNC properties through the in situ deposition of cellulose phosphate oligosaccharides during CNC production.
34260208	3	44	theme	CNC	595:597	arg1	properties					599:608	CNC properties	595:608	CNC properties	595:608	Presented here is a novel surface modification method with the potential to tune CNC properties through the in situ deposition of cellulose phosphate oligosaccharides during CNC production.
34260208	6	45	theme	CNC	1120:1122	arg1	coverage					1132:1139	CNC surface coverage	1120:1139	CNC surface coverage	1120:1139	CNC surface coverage was tuned by controlling the oligosaccharide degree of polymerization.
34260208	5	46	theme	subtle	973:978	arg1	densities					1020:1028	higher surface charge densities	998:1028	higher surface charge densities	998:1028	Oligosaccharide-coated CNCs demonstrated subtle changes including higher surface charge densities and lower water adsorption capacities and viscosities than their unmodified counterparts.
34260208	5	46	theme	subtle	973:978	arg1	changes					980:986	subtle changes	973:986	subtle changes including higher surface charge densities and lower water adsorption capacities	973:1066	Oligosaccharide-coated CNCs demonstrated subtle changes including higher surface charge densities and lower water adsorption capacities and viscosities than their unmodified counterparts.
34260208	5	46	theme	subtle	973:978	arg1	capacities					1057:1066	lower water adsorption capacities	1034:1066	lower water adsorption capacities	1034:1066	Oligosaccharide-coated CNCs demonstrated subtle changes including higher surface charge densities and lower water adsorption capacities and viscosities than their unmodified counterparts.
34260208	4	47	theme	CNC	824:826	arg1	hydrolysis					828:837	the CNC hydrolysis	820:837	the CNC hydrolysis	820:837	This was achieved by leveraging the selective solubility of oligosaccharides, which are soluble at a low pH (during the CNC hydrolysis) yet become insoluble and precipitate onto CNC surfaces upon increasing pH during quenching.
34260208	5	48	theme	higher	998:1003	arg1	densities					1020:1028	higher surface charge densities	998:1028	higher surface charge densities	998:1028	Oligosaccharide-coated CNCs demonstrated subtle changes including higher surface charge densities and lower water adsorption capacities and viscosities than their unmodified counterparts.
34260208	0	49	theme	In	75:76	arg1	Method					120:125	an In Situ Oligosaccharide Surface Modification Method	72:125	an In Situ Oligosaccharide Surface Modification Method	72:125	Tuning the Physicochemical Properties of Cellulose Nanocrystals through an In Situ Oligosaccharide Surface Modification Method.
34260208	6	50	theme	surface	1124:1130	arg1	coverage					1132:1139	CNC surface coverage	1120:1139	CNC surface coverage	1120:1139	CNC surface coverage was tuned by controlling the oligosaccharide degree of polymerization.
34260208	3	51	theme	cellulose	644:652	arg1	oligosaccharides					664:679	cellulose phosphate oligosaccharides	644:679	cellulose phosphate oligosaccharides	644:679	Presented here is a novel surface modification method with the potential to tune CNC properties through the in situ deposition of cellulose phosphate oligosaccharides during CNC production.
34260208	1	52	theme	petroleum-based	149:163	arg1	products					165:172	petroleum-based products	149:172	petroleum-based products	149:172	The trend to replace petroleum-based products with sustainable alternatives has shifted research efforts toward plant-based materials such as cellulose nanocrystals (CNCs).
34260208	1	53	theme	cellulose	270:278	arg1	CNCs					294:297	CNCs	294:297	CNCs	294:297	The trend to replace petroleum-based products with sustainable alternatives has shifted research efforts toward plant-based materials such as cellulose nanocrystals (CNCs).
34260208	1	53	theme	cellulose	270:278	arg1	nanocrystals					280:291	cellulose nanocrystals	270:291	cellulose nanocrystals (CNCs)	270:298	The trend to replace petroleum-based products with sustainable alternatives has shifted research efforts toward plant-based materials such as cellulose nanocrystals (CNCs).
34260208	3	54	theme	phosphate	654:662	arg1	oligosaccharides					664:679	cellulose phosphate oligosaccharides	644:679	cellulose phosphate oligosaccharides	644:679	Presented here is a novel surface modification method with the potential to tune CNC properties through the in situ deposition of cellulose phosphate oligosaccharides during CNC production.
34260208	5	55	theme	Oligosaccharide-coated	932:953	arg1	CNCs					955:958	Oligosaccharide-coated CNCs	932:958	Oligosaccharide-coated CNCs	932:958	Oligosaccharide-coated CNCs demonstrated subtle changes including higher surface charge densities and lower water adsorption capacities and viscosities than their unmodified counterparts.
33047344	7	0	theme	sites	1361:1365	arg1	efficiency					1386:1395	the adsorption efficiency	1371:1395	the adsorption efficiency	1371:1395	The SEM images showed that the surface of the composite membrane was rougher than the porous CTS membrane, which increased the number of adsorption sites and the adsorption efficiency.
33047344	7	0	theme	sites	1361:1365	arg1	number					1340:1345	the number	1336:1345	the number of adsorption sites	1336:1365	The SEM images showed that the surface of the composite membrane was rougher than the porous CTS membrane, which increased the number of adsorption sites and the adsorption efficiency.
33047344	4	1	theme	CTS/β-CDP	925:933	arg1	membrane					945:952	the CTS/β-CDP composite membrane	921:952	the CTS/β-CDP composite membrane	921:952	Comparative studies on the adsorption of CTS membranes, β-CD, β-CDP, and CTS/β-CDP composite membrane showed that the CTS/β-CDP composite membrane had the highest Zn2+ adsorption efficiency.
33047344	8	2	theme	crystal	1505:1511	arg1	structure					1513:1521	the crystal structure	1501:1521	the crystal structure of the CTS	1501:1532	XRD patterns showed that the CTS/β-CDP composite membrane was amorphous, indicating that β-CDP changed the crystal structure of the CTS.
33047344	6	3	theme	CTS/β-CDP	1157:1165	arg1	membrane					1177:1184	the CTS/β-CDP composite membrane	1153:1184	the CTS/β-CDP composite membrane	1153:1184	Characteristic absorption peaks of CTS and β-CDP appeared in the FTIR spectra of the CTS/β-CDP composite membrane, confirming its synthesis.
33047344	7	4	theme	CTS	1306:1308	arg1	membrane					1310:1317	the porous CTS membrane	1295:1317	the porous CTS membrane	1295:1317	The SEM images showed that the surface of the composite membrane was rougher than the porous CTS membrane, which increased the number of adsorption sites and the adsorption efficiency.
33047344	12	5	theme	adsorption	2402:2411	arg1	efficiency					2413:2422	high adsorption efficiency	2397:2422	high adsorption efficiency	2397:2422	After the Zn2+ -containing wastewater underwent pretreatment, the composite membrane was placed into the wastewater for effective adsorption, which could achieve high adsorption efficiency.
33047344	13	6	theme	-containing	2561:2571	arg1	wastewater					2573:2582	Zn2+ -containing wastewater	2556:2582	Zn2+ -containing wastewater	2556:2582	The process played a major role in effectively treating Zn2+ and other difficult to degrade heavy metal ions; thereby, simplifying Zn2+ -containing wastewater.
33047344	9	7	theme	swelling	1539:1546	arg1	degree					1548:1553	The swelling degree	1535:1553	The swelling degree	1535:1553	The swelling degree and transmittance of the CTS/β-CDP composite membrane were lower than the CTS membrane, which should be conducive to recycling after wastewater treatment.
33047344	9	7	theme	swelling	1539:1546	arg1	lower					1614:1618	lower	1614:1618	lower	1614:1618	The swelling degree and transmittance of the CTS/β-CDP composite membrane were lower than the CTS membrane, which should be conducive to recycling after wastewater treatment.
33047344	11	8	theme	chemical	2216:2223	arg1	industry					2225:2232	chemical industry	2216:2232	chemical industry	2216:2232	In this study, CTS and β-CD were cross-linked to synthesize a biomass membrane for adsorbing Zn2+ to reduce the Zn2+ content in wastewater via adsorption.The results show that the CTS/β-CDP composite membrane can be applied to small-scale wastewater treatment fields such as food and chemical industry.
33047344	14	9	theme	removal	2711:2717	arg1	effect					2719:2724	a significant removal effect	2697:2724	a significant removal effect	2697:2724	The treatment process reduces the investment and operating costs of sewage treatment, and at the same time, has a significant removal effect, and hence, can meet the requirements of environmental protection discharge.
33047344	6	10	theme	FTIR	1137:1140	arg1	spectra					1142:1148	the FTIR spectra	1133:1148	the FTIR spectra of the CTS/β-CDP composite membrane	1133:1184	Characteristic absorption peaks of CTS and β-CDP appeared in the FTIR spectra of the CTS/β-CDP composite membrane, confirming its synthesis.
33047344	11	11	theme	composite	2122:2130	arg1	membrane					2132:2139	the CTS/β-CDP composite membrane	2108:2139	the CTS/β-CDP composite membrane	2108:2139	In this study, CTS and β-CD were cross-linked to synthesize a biomass membrane for adsorbing Zn2+ to reduce the Zn2+ content in wastewater via adsorption.The results show that the CTS/β-CDP composite membrane can be applied to small-scale wastewater treatment fields such as food and chemical industry.
33047344	10	12	dep	APPLICATIONS	1720:1731	arg1	harm					1900:1903	harm	1900:1903	can greatly harm the ecosystems and humans	1888:1929	PRACTICAL APPLICATIONS: Industrial wastewater often contains heavy metal ions such as Zn2+ , which are difficult to degrade and are highly toxic, and direct wastewater discharge can greatly harm the ecosystems and humans.
33047344	10	12	dep	APPLICATIONS	1720:1731	arg1	contains					1762:1769	contains	1762:1769	contains heavy metal ions such as Zn2+ , which are difficult to degrade and are highly toxic	1762:1853	PRACTICAL APPLICATIONS: Industrial wastewater often contains heavy metal ions such as Zn2+ , which are difficult to degrade and are highly toxic, and direct wastewater discharge can greatly harm the ecosystems and humans.
33047344	14	13	theme	environmental	2767:2779	arg1	discharge					2792:2800	environmental protection discharge	2767:2800	environmental protection discharge	2767:2800	The treatment process reduces the investment and operating costs of sewage treatment, and at the same time, has a significant removal effect, and hence, can meet the requirements of environmental protection discharge.
33047344	7	14	theme	membrane	1269:1276	arg1	rougher					1282:1288	rougher	1282:1288	rougher	1282:1288	The SEM images showed that the surface of the composite membrane was rougher than the porous CTS membrane, which increased the number of adsorption sites and the adsorption efficiency.
33047344	7	14	theme	membrane	1269:1276	arg1	surface					1244:1250	the surface	1240:1250	the surface of the composite membrane	1240:1276	The SEM images showed that the surface of the composite membrane was rougher than the porous CTS membrane, which increased the number of adsorption sites and the adsorption efficiency.
33047344	11	15	theme	small-scale	2159:2169	arg1	fields					2192:2197	small-scale wastewater treatment fields	2159:2197	small-scale wastewater treatment fields such as food and chemical industry	2159:2232	In this study, CTS and β-CD were cross-linked to synthesize a biomass membrane for adsorbing Zn2+ to reduce the Zn2+ content in wastewater via adsorption.The results show that the CTS/β-CDP composite membrane can be applied to small-scale wastewater treatment fields such as food and chemical industry.
33047344	11	15	theme	small-scale	2159:2169	arg1	food					2207:2210	food	2207:2210	food	2207:2210	In this study, CTS and β-CD were cross-linked to synthesize a biomass membrane for adsorbing Zn2+ to reduce the Zn2+ content in wastewater via adsorption.The results show that the CTS/β-CDP composite membrane can be applied to small-scale wastewater treatment fields such as food and chemical industry.
33047344	11	15	theme	small-scale	2159:2169	arg1	industry					2225:2232	chemical industry	2216:2232	chemical industry	2216:2232	In this study, CTS and β-CD were cross-linked to synthesize a biomass membrane for adsorbing Zn2+ to reduce the Zn2+ content in wastewater via adsorption.The results show that the CTS/β-CDP composite membrane can be applied to small-scale wastewater treatment fields such as food and chemical industry.
33047344	1	16	theme	citric	196:201	arg1	CA					209:210	CA	209:210	CA	209:210	In this study, a β-cyclodextrin polymer (β-CDP) was synthesized by pretreating β-cyclodextrin (β-CD) with citric acid (CA), and then, chitosan (CTS) and β-CDP were cross-linked to prepare a biomass-based (CTS/β-CDP) composite membrane.
33047344	1	16	theme	citric	196:201	arg1	acid					203:206	citric acid	196:206	citric acid (CA)	196:211	In this study, a β-cyclodextrin polymer (β-CDP) was synthesized by pretreating β-cyclodextrin (β-CD) with citric acid (CA), and then, chitosan (CTS) and β-CDP were cross-linked to prepare a biomass-based (CTS/β-CDP) composite membrane.
33047344	9	17	theme	CTS/β-CDP	1580:1588	arg1	membrane					1600:1607	the CTS/β-CDP composite membrane	1576:1607	the CTS/β-CDP composite membrane	1576:1607	The swelling degree and transmittance of the CTS/β-CDP composite membrane were lower than the CTS membrane, which should be conducive to recycling after wastewater treatment.
33047344	14	18	theme	same	2682:2685	arg1	time					2687:2690	the same time	2678:2690	the same time	2678:2690	The treatment process reduces the investment and operating costs of sewage treatment, and at the same time, has a significant removal effect, and hence, can meet the requirements of environmental protection discharge.
33047344	3	19	theme	acid	702:705	arg1	concentration					678:690	the concentration	674:690	the concentration of acetic acid	674:705	The results showed that a maximum adsorption amount 123.7 μg/g and adsorption rate 94.14% of Zn2+ were obtained when the reaction between CTS and β-CDP was performed at 50°C, the concentration of acetic acid was 2%, dissolving β-CDP water dosage was 30 ml, and the soaking time in sodium hydroxide was 1 hr.
33047344	3	19	theme	acid	702:705	arg1	%					712:712	2%	711:712	2%	711:712	The results showed that a maximum adsorption amount 123.7 μg/g and adsorption rate 94.14% of Zn2+ were obtained when the reaction between CTS and β-CDP was performed at 50°C, the concentration of acetic acid was 2%, dissolving β-CDP water dosage was 30 ml, and the soaking time in sodium hydroxide was 1 hr.
33047344	4	20	from	studies	819:825	arg1	β-CD					863:866	β-CD	863:866	β-CD	863:866	Comparative studies on the adsorption of CTS membranes, β-CD, β-CDP, and CTS/β-CDP composite membrane showed that the CTS/β-CDP composite membrane had the highest Zn2+ adsorption efficiency.
33047344	4	20	from	studies	819:825	arg1	adsorption					834:843	the adsorption	830:843	the adsorption of CTS membranes	830:860	Comparative studies on the adsorption of CTS membranes, β-CD, β-CDP, and CTS/β-CDP composite membrane showed that the CTS/β-CDP composite membrane had the highest Zn2+ adsorption efficiency.
33047344	4	20	from	studies	819:825	arg1	membrane					900:907	CTS/β-CDP composite membrane	880:907	CTS/β-CDP composite membrane	880:907	Comparative studies on the adsorption of CTS membranes, β-CD, β-CDP, and CTS/β-CDP composite membrane showed that the CTS/β-CDP composite membrane had the highest Zn2+ adsorption efficiency.
33047344	4	20	from	studies	819:825	arg1	β-CDP					869:873	β-CDP	869:873	β-CDP	869:873	Comparative studies on the adsorption of CTS membranes, β-CD, β-CDP, and CTS/β-CDP composite membrane showed that the CTS/β-CDP composite membrane had the highest Zn2+ adsorption efficiency.
33047344	3	21	theme	amount	544:549	arg1	123.7 μg/g					551:560	a maximum adsorption amount 123.7 μg/g and adsorption rate	523:580	123.7 μg/g	551:560	The results showed that a maximum adsorption amount 123.7 μg/g and adsorption rate 94.14% of Zn2+ were obtained when the reaction between CTS and β-CDP was performed at 50°C, the concentration of acetic acid was 2%, dissolving β-CDP water dosage was 30 ml, and the soaking time in sodium hydroxide was 1 hr.
33047344	9	22	theme	membrane	1600:1607	arg1	degree					1548:1553	The swelling degree	1535:1553	The swelling degree	1535:1553	The swelling degree and transmittance of the CTS/β-CDP composite membrane were lower than the CTS membrane, which should be conducive to recycling after wastewater treatment.
33047344	9	22	theme	membrane	1600:1607	arg1	lower					1614:1618	lower	1614:1618	lower	1614:1618	The swelling degree and transmittance of the CTS/β-CDP composite membrane were lower than the CTS membrane, which should be conducive to recycling after wastewater treatment.
33047344	9	22	theme	membrane	1600:1607	arg1	transmittance					1559:1571	transmittance	1559:1571	transmittance	1559:1571	The swelling degree and transmittance of the CTS/β-CDP composite membrane were lower than the CTS membrane, which should be conducive to recycling after wastewater treatment.
33047344	3	23	theme	maximum	525:531	arg1	123.7 μg/g					551:560	a maximum adsorption amount 123.7 μg/g and adsorption rate	523:580	123.7 μg/g	551:560	The results showed that a maximum adsorption amount 123.7 μg/g and adsorption rate 94.14% of Zn2+ were obtained when the reaction between CTS and β-CDP was performed at 50°C, the concentration of acetic acid was 2%, dissolving β-CDP water dosage was 30 ml, and the soaking time in sodium hydroxide was 1 hr.
33047344	6	24	theme	membrane	1177:1184	arg1	spectra					1142:1148	the FTIR spectra	1133:1148	the FTIR spectra of the CTS/β-CDP composite membrane	1133:1184	Characteristic absorption peaks of CTS and β-CDP appeared in the FTIR spectra of the CTS/β-CDP composite membrane, confirming its synthesis.
33047344	10	25	theme	PRACTICAL	1710:1718	arg1	APPLICATIONS					1720:1731	PRACTICAL APPLICATIONS	1710:1731	PRACTICAL APPLICATIONS: Industrial wastewater often contains heavy metal ions such as Zn2+ , which are difficult to degrade and are highly toxic, and direct wastewater discharge can greatly harm the ecosystems and humans.	1710:1930	PRACTICAL APPLICATIONS: Industrial wastewater often contains heavy metal ions such as Zn2+ , which are difficult to degrade and are highly toxic, and direct wastewater discharge can greatly harm the ecosystems and humans.
33047344	6	26	theme	absorption	1087:1096	arg1	peaks					1098:1102	Characteristic absorption peaks	1072:1102	Characteristic absorption peaks of CTS and β-CDP	1072:1119	Characteristic absorption peaks of CTS and β-CDP appeared in the FTIR spectra of the CTS/β-CDP composite membrane, confirming its synthesis.
33047344	13	27	theme	metal	2523:2527	arg1	ions					2529:2532	heavy metal ions	2517:2532	heavy metal ions	2517:2532	The process played a major role in effectively treating Zn2+ and other difficult to degrade heavy metal ions; thereby, simplifying Zn2+ -containing wastewater.
33047344	5	28	theme	composite	1012:1020	arg1	membrane					1022:1029	The CTS/β-CDP composite membrane	998:1029	The CTS/β-CDP composite membrane	998:1029	The CTS/β-CDP composite membrane was characterized by FTIR, SEM, and XRD.
33047344	2	29	theme	zinc	436:439	arg1	ions					441:444	zinc ions	436:444	zinc ions (Zn2+ ) from simulated wastewater	436:478	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	2	29	theme	zinc	436:439	arg1	Zn2+					447:450	Zn2+	447:450	Zn2+	447:450	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	2	30	from	hydroxide	378:386	arg1	effects					330:336	The effects	326:336	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater	326:478	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	3	31	theme	dosage	738:743	arg1	30 ml					749:753	β-CDP water dosage was 30 ml	726:753	β-CDP water dosage was 30 ml	726:753	The results showed that a maximum adsorption amount 123.7 μg/g and adsorption rate 94.14% of Zn2+ were obtained when the reaction between CTS and β-CDP was performed at 50°C, the concentration of acetic acid was 2%, dissolving β-CDP water dosage was 30 ml, and the soaking time in sodium hydroxide was 1 hr.
33047344	2	32	dep	amount	406:411	arg1	the					391:393	the	391:393	the	391:393	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	3	33	theme	soaking	764:770	arg1	1 hr					801:804	1 hr	801:804	1 hr	801:804	The results showed that a maximum adsorption amount 123.7 μg/g and adsorption rate 94.14% of Zn2+ were obtained when the reaction between CTS and β-CDP was performed at 50°C, the concentration of acetic acid was 2%, dissolving β-CDP water dosage was 30 ml, and the soaking time in sodium hydroxide was 1 hr.
33047344	3	33	theme	soaking	764:770	arg1	time					772:775	the soaking time	760:775	the soaking time in sodium hydroxide	760:795	The results showed that a maximum adsorption amount 123.7 μg/g and adsorption rate 94.14% of Zn2+ were obtained when the reaction between CTS and β-CDP was performed at 50°C, the concentration of acetic acid was 2%, dissolving β-CDP water dosage was 30 ml, and the soaking time in sodium hydroxide was 1 hr.
33047344	2	34	theme	simulated	459:467	arg1	wastewater					469:478	simulated wastewater	459:478	simulated wastewater	459:478	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	3	35	theme	β-CDP	726:730	arg1	30 ml					749:753	β-CDP water dosage was 30 ml	726:753	β-CDP water dosage was 30 ml	726:753	The results showed that a maximum adsorption amount 123.7 μg/g and adsorption rate 94.14% of Zn2+ were obtained when the reaction between CTS and β-CDP was performed at 50°C, the concentration of acetic acid was 2%, dissolving β-CDP water dosage was 30 ml, and the soaking time in sodium hydroxide was 1 hr.
33047344	4	36	theme	membranes	852:860	arg1	β-CD					863:866	β-CD	863:866	β-CD	863:866	Comparative studies on the adsorption of CTS membranes, β-CD, β-CDP, and CTS/β-CDP composite membrane showed that the CTS/β-CDP composite membrane had the highest Zn2+ adsorption efficiency.
33047344	4	36	theme	membranes	852:860	arg1	adsorption					834:843	the adsorption	830:843	the adsorption of CTS membranes	830:860	Comparative studies on the adsorption of CTS membranes, β-CD, β-CDP, and CTS/β-CDP composite membrane showed that the CTS/β-CDP composite membrane had the highest Zn2+ adsorption efficiency.
33047344	4	36	theme	membranes	852:860	arg1	membrane					900:907	CTS/β-CDP composite membrane	880:907	CTS/β-CDP composite membrane	880:907	Comparative studies on the adsorption of CTS membranes, β-CD, β-CDP, and CTS/β-CDP composite membrane showed that the CTS/β-CDP composite membrane had the highest Zn2+ adsorption efficiency.
33047344	4	36	theme	membranes	852:860	arg1	β-CDP					869:873	β-CDP	869:873	β-CDP	869:873	Comparative studies on the adsorption of CTS membranes, β-CD, β-CDP, and CTS/β-CDP composite membrane showed that the CTS/β-CDP composite membrane had the highest Zn2+ adsorption efficiency.
33047344	2	37	from	amount	406:411	arg1	wastewater					469:478	simulated wastewater	459:478	simulated wastewater	459:478	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	7	38	theme	SEM	1217:1219	arg1	images					1221:1226	The SEM images	1213:1226	The SEM images	1213:1226	The SEM images showed that the surface of the composite membrane was rougher than the porous CTS membrane, which increased the number of adsorption sites and the adsorption efficiency.
33047344	2	39	theme	adsorption	395:404	arg1	ions					441:444	zinc ions	436:444	zinc ions (Zn2+ ) from simulated wastewater	436:478	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	2	39	theme	adsorption	395:404	arg1	Zn2+					447:450	Zn2+	447:450	Zn2+	447:450	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	2	39	theme	adsorption	395:404	arg1	amount					406:411	adsorption amount	395:411	adsorption amount	395:411	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	0	40	theme	Zn2+	0:3	arg1	adsorption					5:14	Zn2+ adsorption	0:14	Zn2+ adsorption from wastewater	0:30	Zn2+ adsorption from wastewater using a chitosan/β-cyclodextrin-based composite membrane.
33047344	11	41	theme	biomass	1994:2000	arg1	membrane					2002:2009	a biomass membrane	1992:2009	a biomass membrane for adsorbing Zn2+ to reduce the Zn2+ content in wastewater via adsorption.The results show that the CTS/β-CDP composite membrane can be applied to small-scale wastewater treatment fields such as food and chemical industry	1992:2232	In this study, CTS and β-CD were cross-linked to synthesize a biomass membrane for adsorbing Zn2+ to reduce the Zn2+ content in wastewater via adsorption.The results show that the CTS/β-CDP composite membrane can be applied to small-scale wastewater treatment fields such as food and chemical industry.
33047344	14	42	theme	sewage	2653:2658	arg1	treatment					2660:2668	sewage treatment	2653:2668	sewage treatment	2653:2668	The treatment process reduces the investment and operating costs of sewage treatment, and at the same time, has a significant removal effect, and hence, can meet the requirements of environmental protection discharge.
33047344	2	43	theme	sodium	371:376	arg1	hydroxide					378:386	sodium hydroxide	371:386	sodium hydroxide	371:386	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	1	44	theme	β-cyclodextrin	107:120	arg1	β-CDP					131:135	β-CDP	131:135	β-CDP	131:135	In this study, a β-cyclodextrin polymer (β-CDP) was synthesized by pretreating β-cyclodextrin (β-CD) with citric acid (CA), and then, chitosan (CTS) and β-CDP were cross-linked to prepare a biomass-based (CTS/β-CDP) composite membrane.
33047344	1	44	theme	β-cyclodextrin	107:120	arg1	polymer					122:128	a β-cyclodextrin polymer	105:128	a β-cyclodextrin polymer (β-CDP)	105:136	In this study, a β-cyclodextrin polymer (β-CDP) was synthesized by pretreating β-cyclodextrin (β-CD) with citric acid (CA), and then, chitosan (CTS) and β-CDP were cross-linked to prepare a biomass-based (CTS/β-CDP) composite membrane.
33047344	11	45	theme	Zn2+	2044:2047	arg1	content					2049:2055	the Zn2+ content	2040:2055	the Zn2+ content in wastewater	2040:2069	In this study, CTS and β-CD were cross-linked to synthesize a biomass membrane for adsorbing Zn2+ to reduce the Zn2+ content in wastewater via adsorption.The results show that the CTS/β-CDP composite membrane can be applied to small-scale wastewater treatment fields such as food and chemical industry.
33047344	8	46	theme	CTS/β-CDP	1427:1435	arg1	amorphous					1460:1468	amorphous	1460:1468	amorphous	1460:1468	XRD patterns showed that the CTS/β-CDP composite membrane was amorphous, indicating that β-CDP changed the crystal structure of the CTS.
33047344	8	46	theme	CTS/β-CDP	1427:1435	arg1	membrane					1447:1454	the CTS/β-CDP composite membrane	1423:1454	the CTS/β-CDP composite membrane	1423:1454	XRD patterns showed that the CTS/β-CDP composite membrane was amorphous, indicating that β-CDP changed the crystal structure of the CTS.
33047344	3	47	theme	Zn2+	592:595	arg1	123.7 μg/g					551:560	a maximum adsorption amount 123.7 μg/g and adsorption rate	523:580	123.7 μg/g	551:560	The results showed that a maximum adsorption amount 123.7 μg/g and adsorption rate 94.14% of Zn2+ were obtained when the reaction between CTS and β-CDP was performed at 50°C, the concentration of acetic acid was 2%, dissolving β-CDP water dosage was 30 ml, and the soaking time in sodium hydroxide was 1 hr.
33047344	3	47	theme	Zn2+	592:595	arg1	rate					577:580	a maximum adsorption amount 123.7 μg/g and adsorption rate	523:580	rate	577:580	The results showed that a maximum adsorption amount 123.7 μg/g and adsorption rate 94.14% of Zn2+ were obtained when the reaction between CTS and β-CDP was performed at 50°C, the concentration of acetic acid was 2%, dissolving β-CDP water dosage was 30 ml, and the soaking time in sodium hydroxide was 1 hr.
33047344	2	48	theme	conditions	357:366	arg1	effects					330:336	The effects	326:336	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater	326:478	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	13	49	theme	major	2446:2450	arg1	role					2452:2455	a major role	2444:2455	a major role	2444:2455	The process played a major role in effectively treating Zn2+ and other difficult to degrade heavy metal ions; thereby, simplifying Zn2+ -containing wastewater.
33047344	2	50	from	effects	330:336	arg1	ions					441:444	zinc ions	436:444	zinc ions (Zn2+ ) from simulated wastewater	436:478	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	2	50	from	effects	330:336	arg1	hydroxide					378:386	sodium hydroxide	371:386	sodium hydroxide	371:386	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	2	50	from	effects	330:336	arg1	Zn2+					447:450	Zn2+	447:450	Zn2+	447:450	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	2	50	from	effects	330:336	arg1	amount					406:411	adsorption amount	395:411	adsorption amount	395:411	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	2	50	from	effects	330:336	arg1	rate					428:431	adsorption rate	417:431	adsorption rate	417:431	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	14	51	theme	treatment	2589:2597	arg1	process					2599:2605	The treatment process	2585:2605	The treatment process	2585:2605	The treatment process reduces the investment and operating costs of sewage treatment, and at the same time, has a significant removal effect, and hence, can meet the requirements of environmental protection discharge.
33047344	4	52	theme	composite	890:898	arg1	membrane					900:907	CTS/β-CDP composite membrane	880:907	CTS/β-CDP composite membrane	880:907	Comparative studies on the adsorption of CTS membranes, β-CD, β-CDP, and CTS/β-CDP composite membrane showed that the CTS/β-CDP composite membrane had the highest Zn2+ adsorption efficiency.
33047344	2	53	theme	ions	441:444	arg1	ions					441:444	zinc ions	436:444	zinc ions (Zn2+ ) from simulated wastewater	436:478	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	2	53	theme	ions	441:444	arg1	Zn2+					447:450	Zn2+	447:450	Zn2+	447:450	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	2	53	theme	ions	441:444	arg1	amount					406:411	adsorption amount	395:411	adsorption amount	395:411	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	2	53	theme	ions	441:444	arg1	rate					428:431	adsorption rate	417:431	adsorption rate	417:431	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	0	54	theme	chitosan/β-cyclodextrin-based	40:68	arg1	membrane					80:87	a chitosan/β-cyclodextrin-based composite membrane	38:87	a chitosan/β-cyclodextrin-based composite membrane	38:87	Zn2+ adsorption from wastewater using a chitosan/β-cyclodextrin-based composite membrane.
33047344	7	55	theme	adsorption	1375:1384	arg1	efficiency					1386:1395	the adsorption efficiency	1371:1395	the adsorption efficiency	1371:1395	The SEM images showed that the surface of the composite membrane was rougher than the porous CTS membrane, which increased the number of adsorption sites and the adsorption efficiency.
33047344	14	56	theme	discharge	2792:2800	arg1	requirements					2751:2762	the requirements	2747:2762	the requirements of environmental protection discharge	2747:2800	The treatment process reduces the investment and operating costs of sewage treatment, and at the same time, has a significant removal effect, and hence, can meet the requirements of environmental protection discharge.
33047344	4	57	theme	highest	962:968	arg1	efficiency					986:995	the highest Zn2+ adsorption efficiency	958:995	the highest Zn2+ adsorption efficiency	958:995	Comparative studies on the adsorption of CTS membranes, β-CD, β-CDP, and CTS/β-CDP composite membrane showed that the CTS/β-CDP composite membrane had the highest Zn2+ adsorption efficiency.
33047344	10	58	theme	metal	1777:1781	arg1	Zn2+					1796:1799	Zn2+	1796:1799	Zn2+	1796:1799	PRACTICAL APPLICATIONS: Industrial wastewater often contains heavy metal ions such as Zn2+ , which are difficult to degrade and are highly toxic, and direct wastewater discharge can greatly harm the ecosystems and humans.
33047344	10	58	theme	metal	1777:1781	arg1	ions					1783:1786	heavy metal ions	1771:1786	heavy metal ions	1771:1786	PRACTICAL APPLICATIONS: Industrial wastewater often contains heavy metal ions such as Zn2+ , which are difficult to degrade and are highly toxic, and direct wastewater discharge can greatly harm the ecosystems and humans.
33047344	14	59	theme	significant	2699:2709	arg1	effect					2719:2724	a significant removal effect	2697:2724	a significant removal effect	2697:2724	The treatment process reduces the investment and operating costs of sewage treatment, and at the same time, has a significant removal effect, and hence, can meet the requirements of environmental protection discharge.
33047344	2	60	from	conditions	357:366	arg1	hydroxide					378:386	sodium hydroxide	371:386	sodium hydroxide	371:386	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	4	61	theme	adsorption	975:984	arg1	efficiency					986:995	the highest Zn2+ adsorption efficiency	958:995	the highest Zn2+ adsorption efficiency	958:995	Comparative studies on the adsorption of CTS membranes, β-CD, β-CDP, and CTS/β-CDP composite membrane showed that the CTS/β-CDP composite membrane had the highest Zn2+ adsorption efficiency.
33047344	2	62	from	wastewater	469:478	arg1	ions					441:444	zinc ions	436:444	zinc ions (Zn2+ ) from simulated wastewater	436:478	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	2	62	from	wastewater	469:478	arg1	Zn2+					447:450	Zn2+	447:450	Zn2+	447:450	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	2	62	from	wastewater	469:478	arg1	amount					406:411	adsorption amount	395:411	adsorption amount	395:411	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	2	62	from	wastewater	469:478	arg1	rate					428:431	adsorption rate	417:431	adsorption rate	417:431	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	7	63	theme	adsorption	1350:1359	arg1	sites					1361:1365	adsorption sites	1350:1365	adsorption sites	1350:1365	The SEM images showed that the surface of the composite membrane was rougher than the porous CTS membrane, which increased the number of adsorption sites and the adsorption efficiency.
33047344	11	64	attach	cross-linked	1965:1976	arg3	study					1940:1944	this study	1935:1944	this study	1935:1944	In this study, CTS and β-CD were cross-linked to synthesize a biomass membrane for adsorbing Zn2+ to reduce the Zn2+ content in wastewater via adsorption.The results show that the CTS/β-CDP composite membrane can be applied to small-scale wastewater treatment fields such as food and chemical industry.
33047344	10	65	contain	contains	1762:1769	arg1	wastewater					1745:1754	Industrial wastewater	1734:1754	Industrial wastewater	1734:1754	PRACTICAL APPLICATIONS: Industrial wastewater often contains heavy metal ions such as Zn2+ , which are difficult to degrade and are highly toxic, and direct wastewater discharge can greatly harm the ecosystems and humans.
33047344	10	65	contain	contains	1762:1769	arg2	ions					1783:1786	heavy metal ions	1771:1786	heavy metal ions	1771:1786	PRACTICAL APPLICATIONS: Industrial wastewater often contains heavy metal ions such as Zn2+ , which are difficult to degrade and are highly toxic, and direct wastewater discharge can greatly harm the ecosystems and humans.
33047344	10	65	contain	contains	1762:1769	arg2	Zn2+					1796:1799	Zn2+	1796:1799	Zn2+	1796:1799	PRACTICAL APPLICATIONS: Industrial wastewater often contains heavy metal ions such as Zn2+ , which are difficult to degrade and are highly toxic, and direct wastewater discharge can greatly harm the ecosystems and humans.
33047344	12	66	theme	wastewater	2262:2271	arg1	pretreatment					2283:2294	the Zn2+ -containing wastewater underwent pretreatment	2241:2294	the Zn2+ -containing wastewater underwent pretreatment	2241:2294	After the Zn2+ -containing wastewater underwent pretreatment, the composite membrane was placed into the wastewater for effective adsorption, which could achieve high adsorption efficiency.
33047344	6	67	theme	composite	1167:1175	arg1	membrane					1177:1184	the CTS/β-CDP composite membrane	1153:1184	the CTS/β-CDP composite membrane	1153:1184	Characteristic absorption peaks of CTS and β-CDP appeared in the FTIR spectra of the CTS/β-CDP composite membrane, confirming its synthesis.
33047344	11	68	from	content	2049:2055	arg1	wastewater					2060:2069	wastewater	2060:2069	wastewater	2060:2069	In this study, CTS and β-CD were cross-linked to synthesize a biomass membrane for adsorbing Zn2+ to reduce the Zn2+ content in wastewater via adsorption.The results show that the CTS/β-CDP composite membrane can be applied to small-scale wastewater treatment fields such as food and chemical industry.
33047344	3	69	from	time	772:775	arg1	hydroxide					787:795	sodium hydroxide	780:795	sodium hydroxide	780:795	The results showed that a maximum adsorption amount 123.7 μg/g and adsorption rate 94.14% of Zn2+ were obtained when the reaction between CTS and β-CDP was performed at 50°C, the concentration of acetic acid was 2%, dissolving β-CDP water dosage was 30 ml, and the soaking time in sodium hydroxide was 1 hr.
33047344	12	70	theme	high	2397:2400	arg1	efficiency					2413:2422	high adsorption efficiency	2397:2422	high adsorption efficiency	2397:2422	After the Zn2+ -containing wastewater underwent pretreatment, the composite membrane was placed into the wastewater for effective adsorption, which could achieve high adsorption efficiency.
33047344	7	71	theme	porous	1299:1304	arg1	membrane					1310:1317	the porous CTS membrane	1295:1317	the porous CTS membrane	1295:1317	The SEM images showed that the surface of the composite membrane was rougher than the porous CTS membrane, which increased the number of adsorption sites and the adsorption efficiency.
33047344	11	72	theme	CTS/β-CDP	2112:2120	arg1	membrane					2132:2139	the CTS/β-CDP composite membrane	2108:2139	the CTS/β-CDP composite membrane	2108:2139	In this study, CTS and β-CD were cross-linked to synthesize a biomass membrane for adsorbing Zn2+ to reduce the Zn2+ content in wastewater via adsorption.The results show that the CTS/β-CDP composite membrane can be applied to small-scale wastewater treatment fields such as food and chemical industry.
33047344	14	73	theme	protection	2781:2790	arg1	discharge					2792:2800	environmental protection discharge	2767:2800	environmental protection discharge	2767:2800	The treatment process reduces the investment and operating costs of sewage treatment, and at the same time, has a significant removal effect, and hence, can meet the requirements of environmental protection discharge.
33047344	14	74	from	time	2687:2690	arg1	investment					2619:2628	investment	2619:2628	investment	2619:2628	The treatment process reduces the investment and operating costs of sewage treatment, and at the same time, has a significant removal effect, and hence, can meet the requirements of environmental protection discharge.
33047344	14	74	from	time	2687:2690	arg1	costs					2644:2648	operating costs	2634:2648	operating costs	2634:2648	The treatment process reduces the investment and operating costs of sewage treatment, and at the same time, has a significant removal effect, and hence, can meet the requirements of environmental protection discharge.
33047344	4	75	contain	had	954:956	arg1	membrane					945:952	the CTS/β-CDP composite membrane	921:952	the CTS/β-CDP composite membrane	921:952	Comparative studies on the adsorption of CTS membranes, β-CD, β-CDP, and CTS/β-CDP composite membrane showed that the CTS/β-CDP composite membrane had the highest Zn2+ adsorption efficiency.
33047344	4	75	contain	had	954:956	arg2	efficiency					986:995	the highest Zn2+ adsorption efficiency	958:995	the highest Zn2+ adsorption efficiency	958:995	Comparative studies on the adsorption of CTS membranes, β-CD, β-CDP, and CTS/β-CDP composite membrane showed that the CTS/β-CDP composite membrane had the highest Zn2+ adsorption efficiency.
33047344	3	76	dep	123.7 μg/g	551:560	arg1	%					587:587	94.14%	582:587	a maximum adsorption amount 123.7 μg/g and adsorption rate 94.14% of Zn2+	523:595	The results showed that a maximum adsorption amount 123.7 μg/g and adsorption rate 94.14% of Zn2+ were obtained when the reaction between CTS and β-CDP was performed at 50°C, the concentration of acetic acid was 2%, dissolving β-CDP water dosage was 30 ml, and the soaking time in sodium hydroxide was 1 hr.
33047344	4	77	theme	Comparative	807:817	arg1	studies					819:825	Comparative studies	807:825	Comparative studies on the adsorption of CTS membranes, β-CD, β-CDP, and CTS/β-CDP composite membrane	807:907	Comparative studies on the adsorption of CTS membranes, β-CD, β-CDP, and CTS/β-CDP composite membrane showed that the CTS/β-CDP composite membrane had the highest Zn2+ adsorption efficiency.
33047344	12	78	theme	effective	2355:2363	arg1	adsorption					2365:2374	effective adsorption	2355:2374	effective adsorption	2355:2374	After the Zn2+ -containing wastewater underwent pretreatment, the composite membrane was placed into the wastewater for effective adsorption, which could achieve high adsorption efficiency.
33047344	14	79	dep	investment	2619:2628	arg1	the					2615:2617	the	2615:2617	the	2615:2617	The treatment process reduces the investment and operating costs of sewage treatment, and at the same time, has a significant removal effect, and hence, can meet the requirements of environmental protection discharge.
33047344	3	80	theme	adsorption	533:542	arg1	123.7 μg/g					551:560	a maximum adsorption amount 123.7 μg/g and adsorption rate	523:580	123.7 μg/g	551:560	The results showed that a maximum adsorption amount 123.7 μg/g and adsorption rate 94.14% of Zn2+ were obtained when the reaction between CTS and β-CDP was performed at 50°C, the concentration of acetic acid was 2%, dissolving β-CDP water dosage was 30 ml, and the soaking time in sodium hydroxide was 1 hr.
33047344	13	81	theme	Zn2+	2556:2559	arg1	wastewater					2573:2582	Zn2+ -containing wastewater	2556:2582	Zn2+ -containing wastewater	2556:2582	The process played a major role in effectively treating Zn2+ and other difficult to degrade heavy metal ions; thereby, simplifying Zn2+ -containing wastewater.
33047344	9	82	theme	composite	1590:1598	arg1	membrane					1600:1607	the CTS/β-CDP composite membrane	1576:1607	the CTS/β-CDP composite membrane	1576:1607	The swelling degree and transmittance of the CTS/β-CDP composite membrane were lower than the CTS membrane, which should be conducive to recycling after wastewater treatment.
33047344	7	83	theme	composite	1259:1267	arg1	membrane					1269:1276	the composite membrane	1255:1276	the composite membrane	1255:1276	The SEM images showed that the surface of the composite membrane was rougher than the porous CTS membrane, which increased the number of adsorption sites and the adsorption efficiency.
33047344	6	84	theme	CTS	1107:1109	arg1	peaks					1098:1102	Characteristic absorption peaks	1072:1102	Characteristic absorption peaks of CTS and β-CDP	1072:1119	Characteristic absorption peaks of CTS and β-CDP appeared in the FTIR spectra of the CTS/β-CDP composite membrane, confirming its synthesis.
33047344	3	85	theme	acetic	695:700	arg1	acid					702:705	acetic acid	695:705	acetic acid	695:705	The results showed that a maximum adsorption amount 123.7 μg/g and adsorption rate 94.14% of Zn2+ were obtained when the reaction between CTS and β-CDP was performed at 50°C, the concentration of acetic acid was 2%, dissolving β-CDP water dosage was 30 ml, and the soaking time in sodium hydroxide was 1 hr.
33047344	3	86	theme	adsorption	566:575	arg1	rate					577:580	a maximum adsorption amount 123.7 μg/g and adsorption rate	523:580	rate	577:580	The results showed that a maximum adsorption amount 123.7 μg/g and adsorption rate 94.14% of Zn2+ were obtained when the reaction between CTS and β-CDP was performed at 50°C, the concentration of acetic acid was 2%, dissolving β-CDP water dosage was 30 ml, and the soaking time in sodium hydroxide was 1 hr.
33047344	14	87	theme	treatment	2660:2668	arg1	investment					2619:2628	investment	2619:2628	investment	2619:2628	The treatment process reduces the investment and operating costs of sewage treatment, and at the same time, has a significant removal effect, and hence, can meet the requirements of environmental protection discharge.
33047344	14	87	theme	treatment	2660:2668	arg1	costs					2644:2648	operating costs	2634:2648	operating costs	2634:2648	The treatment process reduces the investment and operating costs of sewage treatment, and at the same time, has a significant removal effect, and hence, can meet the requirements of environmental protection discharge.
33047344	13	88	theme	heavy	2517:2521	arg1	ions					2529:2532	heavy metal ions	2517:2532	heavy metal ions	2517:2532	The process played a major role in effectively treating Zn2+ and other difficult to degrade heavy metal ions; thereby, simplifying Zn2+ -containing wastewater.
33047344	9	89	theme	CTS	1629:1631	arg1	membrane					1633:1640	the CTS membrane	1625:1640	the CTS membrane	1625:1640	The swelling degree and transmittance of the CTS/β-CDP composite membrane were lower than the CTS membrane, which should be conducive to recycling after wastewater treatment.
33047344	9	89	theme	CTS	1629:1631	arg1	conducive					1659:1667	conducive	1659:1667	conducive	1659:1667	The swelling degree and transmittance of the CTS/β-CDP composite membrane were lower than the CTS membrane, which should be conducive to recycling after wastewater treatment.
33047344	0	90	from	wastewater	21:30	arg1	adsorption					5:14	Zn2+ adsorption	0:14	Zn2+ adsorption from wastewater	0:30	Zn2+ adsorption from wastewater using a chitosan/β-cyclodextrin-based composite membrane.
33047344	6	91	theme	Characteristic	1072:1085	arg1	peaks					1098:1102	Characteristic absorption peaks	1072:1102	Characteristic absorption peaks of CTS and β-CDP	1072:1119	Characteristic absorption peaks of CTS and β-CDP appeared in the FTIR spectra of the CTS/β-CDP composite membrane, confirming its synthesis.
33047344	10	92	theme	wastewater	1867:1876	arg1	discharge					1878:1886	direct wastewater discharge	1860:1886	direct wastewater discharge	1860:1886	PRACTICAL APPLICATIONS: Industrial wastewater often contains heavy metal ions such as Zn2+ , which are difficult to degrade and are highly toxic, and direct wastewater discharge can greatly harm the ecosystems and humans.
33047344	1	93	theme	biomass-based	280:292	arg1	CTS/β-CDP					295:303	CTS/β-CDP	295:303	CTS/β-CDP	295:303	In this study, a β-cyclodextrin polymer (β-CDP) was synthesized by pretreating β-cyclodextrin (β-CD) with citric acid (CA), and then, chitosan (CTS) and β-CDP were cross-linked to prepare a biomass-based (CTS/β-CDP) composite membrane.
33047344	1	93	theme	biomass-based	280:292	arg1	membrane					316:323	a biomass-based (CTS/β-CDP) composite membrane	278:323	a biomass-based (CTS/β-CDP) composite membrane	278:323	In this study, a β-cyclodextrin polymer (β-CDP) was synthesized by pretreating β-cyclodextrin (β-CD) with citric acid (CA), and then, chitosan (CTS) and β-CDP were cross-linked to prepare a biomass-based (CTS/β-CDP) composite membrane.
33047344	3	94	theme	sodium	780:785	arg1	hydroxide					787:795	sodium hydroxide	780:795	sodium hydroxide	780:795	The results showed that a maximum adsorption amount 123.7 μg/g and adsorption rate 94.14% of Zn2+ were obtained when the reaction between CTS and β-CDP was performed at 50°C, the concentration of acetic acid was 2%, dissolving β-CDP water dosage was 30 ml, and the soaking time in sodium hydroxide was 1 hr.
33047344	3	95	theme	was	745:747	arg1	30 ml					749:753	β-CDP water dosage was 30 ml	726:753	β-CDP water dosage was 30 ml	726:753	The results showed that a maximum adsorption amount 123.7 μg/g and adsorption rate 94.14% of Zn2+ were obtained when the reaction between CTS and β-CDP was performed at 50°C, the concentration of acetic acid was 2%, dissolving β-CDP water dosage was 30 ml, and the soaking time in sodium hydroxide was 1 hr.
33047344	8	96	theme	XRD	1398:1400	arg1	patterns					1402:1409	XRD patterns	1398:1409	XRD patterns	1398:1409	XRD patterns showed that the CTS/β-CDP composite membrane was amorphous, indicating that β-CDP changed the crystal structure of the CTS.
33047344	3	97	theme	water	732:736	arg1	30 ml					749:753	β-CDP water dosage was 30 ml	726:753	β-CDP water dosage was 30 ml	726:753	The results showed that a maximum adsorption amount 123.7 μg/g and adsorption rate 94.14% of Zn2+ were obtained when the reaction between CTS and β-CDP was performed at 50°C, the concentration of acetic acid was 2%, dissolving β-CDP water dosage was 30 ml, and the soaking time in sodium hydroxide was 1 hr.
33047344	1	98	theme	composite	306:314	arg1	CTS/β-CDP					295:303	CTS/β-CDP	295:303	CTS/β-CDP	295:303	In this study, a β-cyclodextrin polymer (β-CDP) was synthesized by pretreating β-cyclodextrin (β-CD) with citric acid (CA), and then, chitosan (CTS) and β-CDP were cross-linked to prepare a biomass-based (CTS/β-CDP) composite membrane.
33047344	1	98	theme	composite	306:314	arg1	membrane					316:323	a biomass-based (CTS/β-CDP) composite membrane	278:323	a biomass-based (CTS/β-CDP) composite membrane	278:323	In this study, a β-cyclodextrin polymer (β-CDP) was synthesized by pretreating β-cyclodextrin (β-CD) with citric acid (CA), and then, chitosan (CTS) and β-CDP were cross-linked to prepare a biomass-based (CTS/β-CDP) composite membrane.
33047344	11	99	theme	wastewater	2171:2180	arg1	fields					2192:2197	small-scale wastewater treatment fields	2159:2197	small-scale wastewater treatment fields such as food and chemical industry	2159:2232	In this study, CTS and β-CD were cross-linked to synthesize a biomass membrane for adsorbing Zn2+ to reduce the Zn2+ content in wastewater via adsorption.The results show that the CTS/β-CDP composite membrane can be applied to small-scale wastewater treatment fields such as food and chemical industry.
33047344	11	99	theme	wastewater	2171:2180	arg1	food					2207:2210	food	2207:2210	food	2207:2210	In this study, CTS and β-CD were cross-linked to synthesize a biomass membrane for adsorbing Zn2+ to reduce the Zn2+ content in wastewater via adsorption.The results show that the CTS/β-CDP composite membrane can be applied to small-scale wastewater treatment fields such as food and chemical industry.
33047344	11	99	theme	wastewater	2171:2180	arg1	industry					2225:2232	chemical industry	2216:2232	chemical industry	2216:2232	In this study, CTS and β-CD were cross-linked to synthesize a biomass membrane for adsorbing Zn2+ to reduce the Zn2+ content in wastewater via adsorption.The results show that the CTS/β-CDP composite membrane can be applied to small-scale wastewater treatment fields such as food and chemical industry.
33047344	4	100	theme	CTS	848:850	arg1	membranes					852:860	CTS membranes	848:860	CTS membranes	848:860	Comparative studies on the adsorption of CTS membranes, β-CD, β-CDP, and CTS/β-CDP composite membrane showed that the CTS/β-CDP composite membrane had the highest Zn2+ adsorption efficiency.
33047344	2	101	from	rate	428:431	arg1	wastewater					469:478	simulated wastewater	459:478	simulated wastewater	459:478	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	12	102	theme	Zn2+	2245:2248	arg1	pretreatment					2283:2294	the Zn2+ -containing wastewater underwent pretreatment	2241:2294	the Zn2+ -containing wastewater underwent pretreatment	2241:2294	After the Zn2+ -containing wastewater underwent pretreatment, the composite membrane was placed into the wastewater for effective adsorption, which could achieve high adsorption efficiency.
33047344	5	103	theme	CTS/β-CDP	1002:1010	arg1	membrane					1022:1029	The CTS/β-CDP composite membrane	998:1029	The CTS/β-CDP composite membrane	998:1029	The CTS/β-CDP composite membrane was characterized by FTIR, SEM, and XRD.
33047344	9	104	theme	wastewater	1688:1697	arg1	treatment					1699:1707	wastewater treatment	1688:1707	wastewater treatment	1688:1707	The swelling degree and transmittance of the CTS/β-CDP composite membrane were lower than the CTS membrane, which should be conducive to recycling after wastewater treatment.
33047344	8	105	theme	composite	1437:1445	arg1	amorphous					1460:1468	amorphous	1460:1468	amorphous	1460:1468	XRD patterns showed that the CTS/β-CDP composite membrane was amorphous, indicating that β-CDP changed the crystal structure of the CTS.
33047344	8	105	theme	composite	1437:1445	arg1	membrane					1447:1454	the CTS/β-CDP composite membrane	1423:1454	the CTS/β-CDP composite membrane	1423:1454	XRD patterns showed that the CTS/β-CDP composite membrane was amorphous, indicating that β-CDP changed the crystal structure of the CTS.
33047344	11	106	theme	treatment	2182:2190	arg1	fields					2192:2197	small-scale wastewater treatment fields	2159:2197	small-scale wastewater treatment fields such as food and chemical industry	2159:2232	In this study, CTS and β-CD were cross-linked to synthesize a biomass membrane for adsorbing Zn2+ to reduce the Zn2+ content in wastewater via adsorption.The results show that the CTS/β-CDP composite membrane can be applied to small-scale wastewater treatment fields such as food and chemical industry.
33047344	11	106	theme	treatment	2182:2190	arg1	food					2207:2210	food	2207:2210	food	2207:2210	In this study, CTS and β-CD were cross-linked to synthesize a biomass membrane for adsorbing Zn2+ to reduce the Zn2+ content in wastewater via adsorption.The results show that the CTS/β-CDP composite membrane can be applied to small-scale wastewater treatment fields such as food and chemical industry.
33047344	11	106	theme	treatment	2182:2190	arg1	industry					2225:2232	chemical industry	2216:2232	chemical industry	2216:2232	In this study, CTS and β-CD were cross-linked to synthesize a biomass membrane for adsorbing Zn2+ to reduce the Zn2+ content in wastewater via adsorption.The results show that the CTS/β-CDP composite membrane can be applied to small-scale wastewater treatment fields such as food and chemical industry.
33047344	2	107	theme	adsorption	417:426	arg1	rate					428:431	adsorption rate	417:431	adsorption rate	417:431	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	11	108	dep	adsorption.The	2075:2088	arg1	show					2098:2101	show	2098:2101	show that the CTS/β-CDP composite membrane can be applied to small-scale wastewater treatment fields such as food and chemical industry	2098:2232	In this study, CTS and β-CD were cross-linked to synthesize a biomass membrane for adsorbing Zn2+ to reduce the Zn2+ content in wastewater via adsorption.The results show that the CTS/β-CDP composite membrane can be applied to small-scale wastewater treatment fields such as food and chemical industry.
33047344	10	109	theme	Industrial	1734:1743	arg1	wastewater					1745:1754	Industrial wastewater	1734:1754	Industrial wastewater	1734:1754	PRACTICAL APPLICATIONS: Industrial wastewater often contains heavy metal ions such as Zn2+ , which are difficult to degrade and are highly toxic, and direct wastewater discharge can greatly harm the ecosystems and humans.
33047344	2	110	theme	preparation	345:355	arg1	conditions					357:366	the preparation conditions	341:366	the preparation conditions in sodium hydroxide	341:386	The effects of the preparation conditions in sodium hydroxide on the adsorption amount and adsorption rate of zinc ions (Zn2+ ) from simulated wastewater were investigated.
33047344	0	111	theme	composite	70:78	arg1	membrane					80:87	a chitosan/β-cyclodextrin-based composite membrane	38:87	a chitosan/β-cyclodextrin-based composite membrane	38:87	Zn2+ adsorption from wastewater using a chitosan/β-cyclodextrin-based composite membrane.
33047344	10	112	theme	direct	1860:1865	arg1	discharge					1878:1886	direct wastewater discharge	1860:1886	direct wastewater discharge	1860:1886	PRACTICAL APPLICATIONS: Industrial wastewater often contains heavy metal ions such as Zn2+ , which are difficult to degrade and are highly toxic, and direct wastewater discharge can greatly harm the ecosystems and humans.
33047344	14	113	contain	has	2693:2695	arg2	effect					2719:2724	a significant removal effect	2697:2724	a significant removal effect	2697:2724	The treatment process reduces the investment and operating costs of sewage treatment, and at the same time, has a significant removal effect, and hence, can meet the requirements of environmental protection discharge.
33047344	14	113	contain	has	2693:2695	arg1	process					2599:2605	The treatment process	2585:2605	The treatment process	2585:2605	The treatment process reduces the investment and operating costs of sewage treatment, and at the same time, has a significant removal effect, and hence, can meet the requirements of environmental protection discharge.
33047344	4	114	theme	CTS/β-CDP	880:888	arg1	membrane					900:907	CTS/β-CDP composite membrane	880:907	CTS/β-CDP composite membrane	880:907	Comparative studies on the adsorption of CTS membranes, β-CD, β-CDP, and CTS/β-CDP composite membrane showed that the CTS/β-CDP composite membrane had the highest Zn2+ adsorption efficiency.
33047344	12	115	theme	composite	2301:2309	arg1	membrane					2311:2318	the composite membrane	2297:2318	the composite membrane	2297:2318	After the Zn2+ -containing wastewater underwent pretreatment, the composite membrane was placed into the wastewater for effective adsorption, which could achieve high adsorption efficiency.
33047344	8	116	theme	CTS	1530:1532	arg1	structure					1513:1521	the crystal structure	1501:1521	the crystal structure of the CTS	1501:1532	XRD patterns showed that the CTS/β-CDP composite membrane was amorphous, indicating that β-CDP changed the crystal structure of the CTS.
33047344	6	117	theme	β-CDP	1115:1119	arg1	peaks					1098:1102	Characteristic absorption peaks	1072:1102	Characteristic absorption peaks of CTS and β-CDP	1072:1119	Characteristic absorption peaks of CTS and β-CDP appeared in the FTIR spectra of the CTS/β-CDP composite membrane, confirming its synthesis.
33047344	14	118	theme	operating	2634:2642	arg1	costs					2644:2648	operating costs	2634:2648	operating costs	2634:2648	The treatment process reduces the investment and operating costs of sewage treatment, and at the same time, has a significant removal effect, and hence, can meet the requirements of environmental protection discharge.
33047344	12	119	theme	-containing	2250:2260	arg1	pretreatment					2283:2294	the Zn2+ -containing wastewater underwent pretreatment	2241:2294	the Zn2+ -containing wastewater underwent pretreatment	2241:2294	After the Zn2+ -containing wastewater underwent pretreatment, the composite membrane was placed into the wastewater for effective adsorption, which could achieve high adsorption efficiency.
33047344	10	120	theme	heavy	1771:1775	arg1	Zn2+					1796:1799	Zn2+	1796:1799	Zn2+	1796:1799	PRACTICAL APPLICATIONS: Industrial wastewater often contains heavy metal ions such as Zn2+ , which are difficult to degrade and are highly toxic, and direct wastewater discharge can greatly harm the ecosystems and humans.
33047344	10	120	theme	heavy	1771:1775	arg1	ions					1783:1786	heavy metal ions	1771:1786	heavy metal ions	1771:1786	PRACTICAL APPLICATIONS: Industrial wastewater often contains heavy metal ions such as Zn2+ , which are difficult to degrade and are highly toxic, and direct wastewater discharge can greatly harm the ecosystems and humans.
33047344	4	121	theme	composite	935:943	arg1	membrane					945:952	the CTS/β-CDP composite membrane	921:952	the CTS/β-CDP composite membrane	921:952	Comparative studies on the adsorption of CTS membranes, β-CD, β-CDP, and CTS/β-CDP composite membrane showed that the CTS/β-CDP composite membrane had the highest Zn2+ adsorption efficiency.
33047344	4	122	theme	Zn2+	970:973	arg1	efficiency					986:995	the highest Zn2+ adsorption efficiency	958:995	the highest Zn2+ adsorption efficiency	958:995	Comparative studies on the adsorption of CTS membranes, β-CD, β-CDP, and CTS/β-CDP composite membrane showed that the CTS/β-CDP composite membrane had the highest Zn2+ adsorption efficiency.
33047344	12	123	theme	underwent	2273:2281	arg1	pretreatment					2283:2294	the Zn2+ -containing wastewater underwent pretreatment	2241:2294	the Zn2+ -containing wastewater underwent pretreatment	2241:2294	After the Zn2+ -containing wastewater underwent pretreatment, the composite membrane was placed into the wastewater for effective adsorption, which could achieve high adsorption efficiency.
33297187	0	0	theme	composites	95:104	arg1	hydrogels					106:114	alginate/nanofibrillated cellulose double network composites hydrogels	45:114	alginate/nanofibrillated cellulose double network composites hydrogels	45:114	Crucial roles of graphene oxide in preparing alginate/nanofibrillated cellulose double network composites hydrogels.
33297187	8	1	theme	hydrogels	1055:1063	arg1	preparation					1011:1021	the preparation	1007:1021	the preparation of crystalline polymer based DN hydrogels to replace the awkward freezing-thawing process, giving inspiration for DN hydrogels design and preparation	1007:1171	Therefore, our approach here represents a facile method for the preparation of crystalline polymer based DN hydrogels to replace the awkward freezing-thawing process, giving inspiration for DN hydrogels design and preparation.
33297187	8	2	theme	polymer	1038:1044	arg1	hydrogels					1055:1063	crystalline polymer based DN hydrogels	1026:1063	crystalline polymer based DN hydrogels	1026:1063	Therefore, our approach here represents a facile method for the preparation of crystalline polymer based DN hydrogels to replace the awkward freezing-thawing process, giving inspiration for DN hydrogels design and preparation.
33297187	0	3	theme	network	87:93	arg1	hydrogels					106:114	alginate/nanofibrillated cellulose double network composites hydrogels	45:114	alginate/nanofibrillated cellulose double network composites hydrogels	45:114	Crucial roles of graphene oxide in preparing alginate/nanofibrillated cellulose double network composites hydrogels.
33297187	6	4	theme	adsorption	831:840	arg1	property					842:849	an efficacious adsorption property	816:849	an efficacious adsorption property towards CV	816:860	Furthermore, the as-prepared DN hydrogel beads exhibited an efficacious adsorption property towards CV.
33297187	1	5	theme	sodium	160:165	arg1	beads					243:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads	160:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO)	160:283	In this study, a novel strategy to prepare sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO) was developed.
33297187	1	6	theme	DN	230:231	arg1	beads					243:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads	160:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO)	160:283	In this study, a novel strategy to prepare sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO) was developed.
33297187	8	7	theme	awkward	1080:1086	arg1	process					1105:1111	the awkward freezing-thawing process	1076:1111	the awkward freezing-thawing process	1076:1111	Therefore, our approach here represents a facile method for the preparation of crystalline polymer based DN hydrogels to replace the awkward freezing-thawing process, giving inspiration for DN hydrogels design and preparation.
33297187	6	8	theme	efficacious	819:829	arg1	property					842:849	an efficacious adsorption property	816:849	an efficacious adsorption property towards CV	816:860	Furthermore, the as-prepared DN hydrogel beads exhibited an efficacious adsorption property towards CV.
33297187	1	9	dep	oxide	274:278	arg1	GO					281:282	GO	281:282	GO	281:282	In this study, a novel strategy to prepare sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO) was developed.
33297187	7	10	theme	adsorption	875:884	arg1	capacity					886:893	The maximum adsorption capacity	863:893	The maximum adsorption capacity of the hydrogels for CV	863:917	The maximum adsorption capacity of the hydrogels for CV was observed as 665 mg g-1.
33297187	7	10	theme	adsorption	875:884	arg1	665 mg g-1					935:944	665 mg g-1	935:944	665 mg g-1	935:944	The maximum adsorption capacity of the hydrogels for CV was observed as 665 mg g-1.
33297187	1	11	theme	hydrogel	234:241	arg1	beads					243:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads	160:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO)	160:283	In this study, a novel strategy to prepare sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO) was developed.
33297187	5	12	theme	networks	749:756	arg1	formation					729:737	the formation	725:737	the formation of double networks	725:756	Results indicated that the introduction of GO could greatly facilitate the formation of double networks.
33297187	2	13	theme	multi-step	323:332	arg1	method					351:356	the multi-step freezing-thawing method	319:356	the multi-step freezing-thawing method	319:356	In comparison with the multi-step freezing-thawing method, this study employs a facile one-step freeze drying method with the presence of GO sheets.
33297187	1	14	theme	alginate	167:174	arg1	beads					243:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads	160:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO)	160:283	In this study, a novel strategy to prepare sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO) was developed.
33297187	8	15	theme	freezing-thawing	1088:1103	arg1	process					1105:1111	the awkward freezing-thawing process	1076:1111	the awkward freezing-thawing process	1076:1111	Therefore, our approach here represents a facile method for the preparation of crystalline polymer based DN hydrogels to replace the awkward freezing-thawing process, giving inspiration for DN hydrogels design and preparation.
33297187	9	16	theme	great	1245:1249	arg1	promise					1251:1257	great promise	1245:1257	great promise for the water pollution control materials	1245:1299	Moreover, due to its efficient adsorption capacity, the hydrogels hold great promise for the water pollution control materials.
33297187	2	17	theme	one-step	387:394	arg1	method					410:415	a facile one-step freeze drying method	378:415	a facile one-step freeze drying method	378:415	In comparison with the multi-step freezing-thawing method, this study employs a facile one-step freeze drying method with the presence of GO sheets.
33297187	2	18	theme	freezing-thawing	334:349	arg1	method					351:356	the multi-step freezing-thawing method	319:356	the multi-step freezing-thawing method	319:356	In comparison with the multi-step freezing-thawing method, this study employs a facile one-step freeze drying method with the presence of GO sheets.
33297187	2	19	theme	freeze	396:401	arg1	method					410:415	a facile one-step freeze drying method	378:415	a facile one-step freeze drying method	378:415	In comparison with the multi-step freezing-thawing method, this study employs a facile one-step freeze drying method with the presence of GO sheets.
33297187	6	20	theme	hydrogel	791:798	arg1	beads					800:804	the as-prepared DN hydrogel beads	772:804	the as-prepared DN hydrogel beads	772:804	Furthermore, the as-prepared DN hydrogel beads exhibited an efficacious adsorption property towards CV.
33297187	8	21	theme	facile	989:994	arg1	method					996:1001	a facile method	987:1001	a facile method for the preparation of crystalline polymer based DN hydrogels to replace the awkward freezing-thawing process, giving inspiration for DN hydrogels design and preparation	987:1171	Therefore, our approach here represents a facile method for the preparation of crystalline polymer based DN hydrogels to replace the awkward freezing-thawing process, giving inspiration for DN hydrogels design and preparation.
33297187	2	22	theme	sheets	441:446	arg1	presence					426:433	the presence	422:433	the presence of GO sheets	422:446	In comparison with the multi-step freezing-thawing method, this study employs a facile one-step freeze drying method with the presence of GO sheets.
33297187	3	23	theme	efficient	496:504	arg1	agent					517:521	an efficient nucleating agent	493:521	an efficient nucleating agent of NFC	493:528	The crucial roles of GO were highlighted as an efficient nucleating agent of NFC and a reinforcer for the hydrogel.
33297187	3	23	theme	efficient	496:504	arg1	roles					461:465	The crucial roles	449:465	The crucial roles of GO	449:471	The crucial roles of GO were highlighted as an efficient nucleating agent of NFC and a reinforcer for the hydrogel.
33297187	1	24	theme	SA	177:178	arg1	beads					243:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads	160:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO)	160:283	In this study, a novel strategy to prepare sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO) was developed.
33297187	0	25	theme	Crucial	0:6	arg1	roles					8:12	Crucial roles	0:12	Crucial roles of graphene oxide in preparing alginate/nanofibrillated cellulose double network composites hydrogels.	0:115	Crucial roles of graphene oxide in preparing alginate/nanofibrillated cellulose double network composites hydrogels.
33297187	9	26	theme	efficient	1195:1203	arg1	capacity					1216:1223	its efficient adsorption capacity	1191:1223	its efficient adsorption capacity	1191:1223	Moreover, due to its efficient adsorption capacity, the hydrogels hold great promise for the water pollution control materials.
33297187	1	27	with	beads	243:247	arg1	the					254:256	the	254:256	the	254:256	In this study, a novel strategy to prepare sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO) was developed.
33297187	3	28	theme	nucleating	506:515	arg1	agent					517:521	an efficient nucleating agent	493:521	an efficient nucleating agent of NFC	493:528	The crucial roles of GO were highlighted as an efficient nucleating agent of NFC and a reinforcer for the hydrogel.
33297187	3	28	theme	nucleating	506:515	arg1	roles					461:465	The crucial roles	449:465	The crucial roles of GO	449:471	The crucial roles of GO were highlighted as an efficient nucleating agent of NFC and a reinforcer for the hydrogel.
33297187	6	29	theme	as-prepared	776:786	arg1	beads					800:804	the as-prepared DN hydrogel beads	772:804	the as-prepared DN hydrogel beads	772:804	Furthermore, the as-prepared DN hydrogel beads exhibited an efficacious adsorption property towards CV.
33297187	0	30	theme	graphene	17:24	arg1	oxide					26:30	graphene oxide	17:30	graphene oxide	17:30	Crucial roles of graphene oxide in preparing alginate/nanofibrillated cellulose double network composites hydrogels.
33297187	1	31	theme	/nano	180:184	arg1	beads					243:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads	160:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO)	160:283	In this study, a novel strategy to prepare sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO) was developed.
33297187	9	32	theme	water	1267:1271	arg1	materials					1291:1299	the water pollution control materials	1263:1299	the water pollution control materials	1263:1299	Moreover, due to its efficient adsorption capacity, the hydrogels hold great promise for the water pollution control materials.
33297187	6	33	theme	DN	788:789	arg1	beads					800:804	the as-prepared DN hydrogel beads	772:804	the as-prepared DN hydrogel beads	772:804	Furthermore, the as-prepared DN hydrogel beads exhibited an efficacious adsorption property towards CV.
33297187	4	34	theme	crystal	616:622	arg1	CV					632:633	CV	632:633	CV	632:633	The adsorption property of the DN hydrogel towards crystal violet (CV) was also studied.
33297187	4	34	theme	crystal	616:622	arg1	violet					624:629	crystal violet	616:629	crystal violet (CV)	616:634	The adsorption property of the DN hydrogel towards crystal violet (CV) was also studied.
33297187	1	35	theme	fibrillated	186:196	arg1	beads					243:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads	160:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO)	160:283	In this study, a novel strategy to prepare sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO) was developed.
33297187	8	36	theme	hydrogels	1140:1148	arg1	design					1150:1155	DN hydrogels design	1137:1155	DN hydrogels design	1137:1155	Therefore, our approach here represents a facile method for the preparation of crystalline polymer based DN hydrogels to replace the awkward freezing-thawing process, giving inspiration for DN hydrogels design and preparation.
33297187	4	37	theme	DN	596:597	arg1	hydrogel					599:606	the DN hydrogel	592:606	the DN hydrogel	592:606	The adsorption property of the DN hydrogel towards crystal violet (CV) was also studied.
33297187	4	38	theme	adsorption	569:578	arg1	property					580:587	The adsorption property	565:587	The adsorption property of the DN hydrogel towards crystal violet (CV)	565:634	The adsorption property of the DN hydrogel towards crystal violet (CV) was also studied.
33297187	3	39	theme	NFC	526:528	arg1	reinforcer					536:545	a reinforcer	534:545	a reinforcer for the hydrogel	534:562	The crucial roles of GO were highlighted as an efficient nucleating agent of NFC and a reinforcer for the hydrogel.
33297187	3	39	theme	NFC	526:528	arg1	agent					517:521	an efficient nucleating agent	493:521	an efficient nucleating agent of NFC	493:528	The crucial roles of GO were highlighted as an efficient nucleating agent of NFC and a reinforcer for the hydrogel.
33297187	3	39	theme	NFC	526:528	arg1	roles					461:465	The crucial roles	449:465	The crucial roles of GO	449:471	The crucial roles of GO were highlighted as an efficient nucleating agent of NFC and a reinforcer for the hydrogel.
33297187	1	40	theme	cellulose	198:206	arg1	beads					243:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads	160:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO)	160:283	In this study, a novel strategy to prepare sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO) was developed.
33297187	0	41	theme	oxide	26:30	arg1	roles					8:12	Crucial roles	0:12	Crucial roles of graphene oxide in preparing alginate/nanofibrillated cellulose double network composites hydrogels.	0:115	Crucial roles of graphene oxide in preparing alginate/nanofibrillated cellulose double network composites hydrogels.
33297187	9	42	theme	control	1283:1289	arg1	materials					1291:1299	the water pollution control materials	1263:1299	the water pollution control materials	1263:1299	Moreover, due to its efficient adsorption capacity, the hydrogels hold great promise for the water pollution control materials.
33297187	5	43	theme	GO	697:698	arg1	introduction					681:692	the introduction	677:692	the introduction of GO	677:698	Results indicated that the introduction of GO could greatly facilitate the formation of double networks.
33297187	2	44	theme	facile	380:385	arg1	method					410:415	a facile one-step freeze drying method	378:415	a facile one-step freeze drying method	378:415	In comparison with the multi-step freezing-thawing method, this study employs a facile one-step freeze drying method with the presence of GO sheets.
33297187	0	45	theme	alginate/nanofibrillated	45:68	arg1	hydrogels					106:114	alginate/nanofibrillated cellulose double network composites hydrogels	45:114	alginate/nanofibrillated cellulose double network composites hydrogels	45:114	Crucial roles of graphene oxide in preparing alginate/nanofibrillated cellulose double network composites hydrogels.
33297187	1	46	theme	graphene	265:272	arg1	oxide					274:278	graphene oxide	265:278	graphene oxide (GO)	265:283	In this study, a novel strategy to prepare sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO) was developed.
33297187	8	47	theme	crystalline	1026:1036	arg1	hydrogels					1055:1063	crystalline polymer based DN hydrogels	1026:1063	crystalline polymer based DN hydrogels	1026:1063	Therefore, our approach here represents a facile method for the preparation of crystalline polymer based DN hydrogels to replace the awkward freezing-thawing process, giving inspiration for DN hydrogels design and preparation.
33297187	8	48	theme	DN	1052:1053	arg1	hydrogels					1055:1063	crystalline polymer based DN hydrogels	1026:1063	crystalline polymer based DN hydrogels	1026:1063	Therefore, our approach here represents a facile method for the preparation of crystalline polymer based DN hydrogels to replace the awkward freezing-thawing process, giving inspiration for DN hydrogels design and preparation.
33297187	8	49	theme	based	1046:1050	arg1	hydrogels					1055:1063	crystalline polymer based DN hydrogels	1026:1063	crystalline polymer based DN hydrogels	1026:1063	Therefore, our approach here represents a facile method for the preparation of crystalline polymer based DN hydrogels to replace the awkward freezing-thawing process, giving inspiration for DN hydrogels design and preparation.
33297187	2	50	theme	drying	403:408	arg1	method					410:415	a facile one-step freeze drying method	378:415	a facile one-step freeze drying method	378:415	In comparison with the multi-step freezing-thawing method, this study employs a facile one-step freeze drying method with the presence of GO sheets.
33297187	7	51	theme	maximum	867:873	arg1	capacity					886:893	The maximum adsorption capacity	863:893	The maximum adsorption capacity of the hydrogels for CV	863:917	The maximum adsorption capacity of the hydrogels for CV was observed as 665 mg g-1.
33297187	7	51	theme	maximum	867:873	arg1	665 mg g-1					935:944	665 mg g-1	935:944	665 mg g-1	935:944	The maximum adsorption capacity of the hydrogels for CV was observed as 665 mg g-1.
33297187	9	52	theme	adsorption	1205:1214	arg1	capacity					1216:1223	its efficient adsorption capacity	1191:1223	its efficient adsorption capacity	1191:1223	Moreover, due to its efficient adsorption capacity, the hydrogels hold great promise for the water pollution control materials.
33297187	2	53	dep	sheets	441:446	arg1	GO					438:439	GO	438:439	GO	438:439	In comparison with the multi-step freezing-thawing method, this study employs a facile one-step freeze drying method with the presence of GO sheets.
33297187	9	54	theme	pollution	1273:1281	arg1	materials					1291:1299	the water pollution control materials	1263:1299	the water pollution control materials	1263:1299	Moreover, due to its efficient adsorption capacity, the hydrogels hold great promise for the water pollution control materials.
33297187	1	55	theme	NFC	209:211	arg1	beads					243:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads	160:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO)	160:283	In this study, a novel strategy to prepare sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO) was developed.
33297187	3	56	theme	GO	470:471	arg1	reinforcer					536:545	a reinforcer	534:545	a reinforcer for the hydrogel	534:562	The crucial roles of GO were highlighted as an efficient nucleating agent of NFC and a reinforcer for the hydrogel.
33297187	3	56	theme	GO	470:471	arg1	agent					517:521	an efficient nucleating agent	493:521	an efficient nucleating agent of NFC	493:528	The crucial roles of GO were highlighted as an efficient nucleating agent of NFC and a reinforcer for the hydrogel.
33297187	3	56	theme	GO	470:471	arg1	roles					461:465	The crucial roles	449:465	The crucial roles of GO	449:471	The crucial roles of GO were highlighted as an efficient nucleating agent of NFC and a reinforcer for the hydrogel.
33297187	1	57	theme	novel	134:138	arg1	strategy					140:147	a novel strategy	132:147	a novel strategy to prepare sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO)	132:283	In this study, a novel strategy to prepare sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO) was developed.
33297187	8	58	theme	DN	1137:1138	arg1	design					1150:1155	DN hydrogels design	1137:1155	DN hydrogels design	1137:1155	Therefore, our approach here represents a facile method for the preparation of crystalline polymer based DN hydrogels to replace the awkward freezing-thawing process, giving inspiration for DN hydrogels design and preparation.
33297187	0	59	theme	double	80:85	arg1	hydrogels					106:114	alginate/nanofibrillated cellulose double network composites hydrogels	45:114	alginate/nanofibrillated cellulose double network composites hydrogels	45:114	Crucial roles of graphene oxide in preparing alginate/nanofibrillated cellulose double network composites hydrogels.
33297187	2	60	with	comparison	303:312	arg1	method					351:356	the multi-step freezing-thawing method	319:356	the multi-step freezing-thawing method	319:356	In comparison with the multi-step freezing-thawing method, this study employs a facile one-step freeze drying method with the presence of GO sheets.
33297187	5	61	theme	double	742:747	arg1	networks					749:756	double networks	742:756	double networks	742:756	Results indicated that the introduction of GO could greatly facilitate the formation of double networks.
33297187	3	62	theme	crucial	453:459	arg1	reinforcer					536:545	a reinforcer	534:545	a reinforcer for the hydrogel	534:562	The crucial roles of GO were highlighted as an efficient nucleating agent of NFC and a reinforcer for the hydrogel.
33297187	3	62	theme	crucial	453:459	arg1	agent					517:521	an efficient nucleating agent	493:521	an efficient nucleating agent of NFC	493:528	The crucial roles of GO were highlighted as an efficient nucleating agent of NFC and a reinforcer for the hydrogel.
33297187	3	62	theme	crucial	453:459	arg1	roles					461:465	The crucial roles	449:465	The crucial roles of GO	449:471	The crucial roles of GO were highlighted as an efficient nucleating agent of NFC and a reinforcer for the hydrogel.
33297187	1	63	theme	double	214:219	arg1	beads					243:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads	160:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO)	160:283	In this study, a novel strategy to prepare sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO) was developed.
33297187	0	64	theme	cellulose	70:78	arg1	hydrogels					106:114	alginate/nanofibrillated cellulose double network composites hydrogels	45:114	alginate/nanofibrillated cellulose double network composites hydrogels	45:114	Crucial roles of graphene oxide in preparing alginate/nanofibrillated cellulose double network composites hydrogels.
33297187	1	65	dep	the	254:256	arg1	aid					258:260	aid	258:260	aid	258:260	In this study, a novel strategy to prepare sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO) was developed.
33297187	7	66	theme	hydrogels	902:910	arg1	capacity					886:893	The maximum adsorption capacity	863:893	The maximum adsorption capacity of the hydrogels for CV	863:917	The maximum adsorption capacity of the hydrogels for CV was observed as 665 mg g-1.
33297187	7	66	theme	hydrogels	902:910	arg1	665 mg g-1					935:944	665 mg g-1	935:944	665 mg g-1	935:944	The maximum adsorption capacity of the hydrogels for CV was observed as 665 mg g-1.
33297187	4	67	theme	hydrogel	599:606	arg1	property					580:587	The adsorption property	565:587	The adsorption property of the DN hydrogel towards crystal violet (CV)	565:634	The adsorption property of the DN hydrogel towards crystal violet (CV) was also studied.
33297187	1	68	theme	network	221:227	arg1	beads					243:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads	160:247	sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO)	160:283	In this study, a novel strategy to prepare sodium alginate (SA)/nano fibrillated cellulose (NFC) double network (DN) hydrogel beads with the aid of graphene oxide (GO) was developed.
33152428	11	0	theme	TGF-β	2018:2022	arg1	secretions					2024:2033	TGF-β secretions	2018:2033	TGF-β secretions	2018:2033	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues are consistent with the variation tendency of intestinal flora.
33152428	12	1	theme	dose-dependent	2184:2197	arg1	regulations					2199:2209	effective, dynamical and dose-dependent regulations	2159:2209	effective, dynamical and dose-dependent regulations on intestinal microenvironment	2159:2240	CONCLUSION CCP can provide effective, dynamical and dose-dependent regulations on intestinal microenvironment, not only the intestinal flora but also the PPs and intestinal epithelium related immune response.
33152428	6	2	theme	labeled	1235:1241	arg1	CCP					1243:1245	FITC labeled CCP	1230:1245	FITC labeled CCP	1230:1245	MATERIALS AND METHODS The absorption and distribution of CCP in intestinal tissues were observed after the perfusion of FITC labeled CCP.
33152428	3	3	from	polysaccharides	514:528	arg1	medicines					540:548	herbal medicines	533:548	herbal medicines	533:548	The polysaccharides in herbal medicines can be digested by the flora and uptaken by the Peyer's patches (PPs) in intestine.
33152428	10	4	used	utilized	1833:1840	arg2	CCP					1817:1819	The CCP	1813:1819	The CCP	1813:1819	The CCP can also be utilized by the intestinal flora and dynamically regulate the diversity, composition and distribution of the intestinal flora.
33152428	9	5	from	IL-17	1752:1756	arg1	PPs					1781:1783	PPs	1781:1783	PPs	1781:1783	RESULTS With the self-aggregation into particles morphology, CCP can be up-taken by PPs and promote the IFN-γ, IL-4, IL-17 and TGF-β secretion in PPs in a dose-dependent manner.
33152428	11	6	theme	epithelial	2049:2058	arg1	tissues					2060:2066	intestinal epithelial tissues	2038:2066	intestinal epithelial tissues	2038:2066	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues are consistent with the variation tendency of intestinal flora.
33152428	6	7	dep	MATERIALS	1110:1118	arg1	absorption					1136:1145	absorption	1136:1145	absorption	1136:1145	MATERIALS AND METHODS The absorption and distribution of CCP in intestinal tissues were observed after the perfusion of FITC labeled CCP.
33152428	6	7	dep	MATERIALS	1110:1118	arg1	MATERIALS					1110:1118	MATERIALS	1110:1118	MATERIALS	1110:1118	MATERIALS AND METHODS The absorption and distribution of CCP in intestinal tissues were observed after the perfusion of FITC labeled CCP.
33152428	6	7	dep	MATERIALS	1110:1118	arg1	distribution					1151:1162	distribution	1151:1162	distribution	1151:1162	MATERIALS AND METHODS The absorption and distribution of CCP in intestinal tissues were observed after the perfusion of FITC labeled CCP.
33152428	5	8	theme	STUDY	821:825	arg1	AIM					810:812	AIM	810:812	AIM OF THE STUDY	810:825	AIM OF THE STUDY This study intended to explore the dynamic regulation of CCP on intestinal microenvironment from the perspective of the intestinal mucosal immunity and the intestinal flora, in order to provide a new research perspective for the pharmacological mechanism of Coptis chinensis Franch.
33152428	9	9	from	IL-4	1746:1749	arg1	PPs					1781:1783	PPs	1781:1783	PPs	1781:1783	RESULTS With the self-aggregation into particles morphology, CCP can be up-taken by PPs and promote the IFN-γ, IL-4, IL-17 and TGF-β secretion in PPs in a dose-dependent manner.
33152428	9	10	dep	IFN-γ	1739:1743	arg1	the					1735:1737	the	1735:1737	the	1735:1737	RESULTS With the self-aggregation into particles morphology, CCP can be up-taken by PPs and promote the IFN-γ, IL-4, IL-17 and TGF-β secretion in PPs in a dose-dependent manner.
33152428	1	11	from	herbs	263:267	arg1	China					272:276	China	272:276	China	272:276	ETHNOPHARMACOLOGICAL RELEVANCE Coptis chinensis Franch is one of the most widely used traditional Chinese herbs in China and was firstly recorded in "Shennong's Classic of Materia Medica" in the Han Dynasty.
33152428	12	12	from	PPs	2286:2288	arg1	microenvironment					2225:2240	intestinal microenvironment	2214:2240	intestinal microenvironment	2214:2240	CONCLUSION CCP can provide effective, dynamical and dose-dependent regulations on intestinal microenvironment, not only the intestinal flora but also the PPs and intestinal epithelium related immune response.
33152428	11	13	theme	temporal	1964:1971	arg1	regulations					1973:1983	The temporal regulations	1960:1983	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues	1960:2066	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues are consistent with the variation tendency of intestinal flora.
33152428	11	13	theme	temporal	1964:1971	arg1	consistent					2072:2081	consistent	2072:2081	consistent	2072:2081	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues are consistent with the variation tendency of intestinal flora.
33152428	11	14	theme	CCP	1988:1990	arg1	regulations					1973:1983	The temporal regulations	1960:1983	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues	1960:2066	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues are consistent with the variation tendency of intestinal flora.
33152428	11	14	theme	CCP	1988:1990	arg1	consistent					2072:2081	consistent	2072:2081	consistent	2072:2081	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues are consistent with the variation tendency of intestinal flora.
33152428	6	15	dep	absorption	1136:1145	arg1	The					1132:1134	The	1132:1134	The	1132:1134	MATERIALS AND METHODS The absorption and distribution of CCP in intestinal tissues were observed after the perfusion of FITC labeled CCP.
33152428	11	16	from	IL-17	2008:2012	arg1	tissues					2060:2066	intestinal epithelial tissues	2038:2066	intestinal epithelial tissues	2038:2066	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues are consistent with the variation tendency of intestinal flora.
33152428	0	17	theme	mucosal	139:145	arg1	immunity					147:154	mucosal immunity	139:154	mucosal immunity	139:154	Coptis chinensis Franch polysaccharides provide a dynamically regulation on intestinal microenvironment, based on the intestinal flora and mucosal immunity.
33152428	5	18	theme	research	1027:1034	arg1	perspective					1036:1046	a new research perspective	1021:1046	a new research perspective for the pharmacological mechanism of Coptis chinensis Franch	1021:1107	AIM OF THE STUDY This study intended to explore the dynamic regulation of CCP on intestinal microenvironment from the perspective of the intestinal mucosal immunity and the intestinal flora, in order to provide a new research perspective for the pharmacological mechanism of Coptis chinensis Franch.
33152428	7	19	theme	illumina-miseq	1324:1337	arg1	sequencing					1339:1348	the 16sRNA gene illumina-miseq sequencing	1308:1348	the 16sRNA gene illumina-miseq sequencing	1308:1348	The influences of CCP on intestinal flora were evaluated by the 16sRNA gene illumina-miseq sequencing after gavage.
33152428	8	20	from	secretion	1584:1592	arg1	PPs					1597:1599	PPs	1597:1599	PPs	1597:1599	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	8	20	from	secretion	1584:1592	arg1	tissue					1627:1632	intestinal epithelial tissue	1605:1632	intestinal epithelial tissue	1605:1632	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	6	21	theme	intestinal	1174:1183	arg1	tissues					1185:1191	intestinal tissues	1174:1191	intestinal tissues	1174:1191	MATERIALS AND METHODS The absorption and distribution of CCP in intestinal tissues were observed after the perfusion of FITC labeled CCP.
33152428	7	22	theme	16sRNA	1312:1317	arg1	sequencing					1339:1348	the 16sRNA gene illumina-miseq sequencing	1308:1348	the 16sRNA gene illumina-miseq sequencing	1308:1348	The influences of CCP on intestinal flora were evaluated by the 16sRNA gene illumina-miseq sequencing after gavage.
33152428	10	23	theme	intestinal	1942:1951	arg1	flora					1953:1957	the intestinal flora	1938:1957	the intestinal flora	1938:1957	The CCP can also be utilized by the intestinal flora and dynamically regulate the diversity, composition and distribution of the intestinal flora.
33152428	5	24	theme	CCP	884:886	arg1	regulation					870:879	the dynamic regulation	858:879	the dynamic regulation of CCP on intestinal microenvironment	858:917	AIM OF THE STUDY This study intended to explore the dynamic regulation of CCP on intestinal microenvironment from the perspective of the intestinal mucosal immunity and the intestinal flora, in order to provide a new research perspective for the pharmacological mechanism of Coptis chinensis Franch.
33152428	6	25	theme	CCP	1167:1169	arg1	absorption					1136:1145	absorption	1136:1145	absorption	1136:1145	MATERIALS AND METHODS The absorption and distribution of CCP in intestinal tissues were observed after the perfusion of FITC labeled CCP.
33152428	6	25	theme	CCP	1167:1169	arg1	MATERIALS					1110:1118	MATERIALS	1110:1118	MATERIALS	1110:1118	MATERIALS AND METHODS The absorption and distribution of CCP in intestinal tissues were observed after the perfusion of FITC labeled CCP.
33152428	6	25	theme	CCP	1167:1169	arg1	distribution					1151:1162	distribution	1151:1162	distribution	1151:1162	MATERIALS AND METHODS The absorption and distribution of CCP in intestinal tissues were observed after the perfusion of FITC labeled CCP.
33152428	11	26	from	IL-4	2002:2005	arg1	tissues					2060:2066	intestinal epithelial tissues	2038:2066	intestinal epithelial tissues	2038:2066	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues are consistent with the variation tendency of intestinal flora.
33152428	1	27	theme	Materia	329:335	arg1	Medica					337:342	Materia Medica	329:342	Materia Medica	329:342	ETHNOPHARMACOLOGICAL RELEVANCE Coptis chinensis Franch is one of the most widely used traditional Chinese herbs in China and was firstly recorded in "Shennong's Classic of Materia Medica" in the Han Dynasty.
33152428	12	28	from	flora	2267:2271	arg1	microenvironment					2225:2240	intestinal microenvironment	2214:2240	intestinal microenvironment	2214:2240	CONCLUSION CCP can provide effective, dynamical and dose-dependent regulations on intestinal microenvironment, not only the intestinal flora but also the PPs and intestinal epithelium related immune response.
33152428	11	29	from	IFN-γ	1995:1999	arg1	tissues					2060:2066	intestinal epithelial tissues	2038:2066	intestinal epithelial tissues	2038:2066	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues are consistent with the variation tendency of intestinal flora.
33152428	11	30	theme	flora	2125:2129	arg1	tendency					2102:2109	the variation tendency	2088:2109	the variation tendency of intestinal flora	2088:2129	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues are consistent with the variation tendency of intestinal flora.
33152428	10	31	dep	diversity	1895:1903	arg1	the					1891:1893	the	1891:1893	the	1891:1893	The CCP can also be utilized by the intestinal flora and dynamically regulate the diversity, composition and distribution of the intestinal flora.
33152428	11	32	from	regulations	1973:1983	arg1	IL-17					2008:2012	IL-17	2008:2012	IL-17	2008:2012	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues are consistent with the variation tendency of intestinal flora.
33152428	11	32	from	regulations	1973:1983	arg1	secretions					2024:2033	TGF-β secretions	2018:2033	TGF-β secretions	2018:2033	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues are consistent with the variation tendency of intestinal flora.
33152428	11	32	from	regulations	1973:1983	arg1	IL-4					2002:2005	IL-4	2002:2005	IL-4	2002:2005	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues are consistent with the variation tendency of intestinal flora.
33152428	11	32	from	regulations	1973:1983	arg1	IFN-γ					1995:1999	IFN-γ	1995:1999	IFN-γ	1995:1999	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues are consistent with the variation tendency of intestinal flora.
33152428	0	33	dep	flora	129:133	arg1	the					114:116	the	114:116	the	114:116	Coptis chinensis Franch polysaccharides provide a dynamically regulation on intestinal microenvironment, based on the intestinal flora and mucosal immunity.
33152428	12	34	theme	CONCLUSION	2132:2141	arg1	CCP					2143:2145	CONCLUSION CCP	2132:2145	CONCLUSION CCP	2132:2145	CONCLUSION CCP can provide effective, dynamical and dose-dependent regulations on intestinal microenvironment, not only the intestinal flora but also the PPs and intestinal epithelium related immune response.
33152428	8	35	from	analysis	1460:1467	arg1	PPs					1597:1599	PPs	1597:1599	PPs	1597:1599	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	8	35	from	analysis	1460:1467	arg1	tissue					1627:1632	intestinal epithelial tissue	1605:1632	intestinal epithelial tissue	1605:1632	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	12	36	theme	dynamical	2170:2178	arg1	regulations					2199:2209	effective, dynamical and dose-dependent regulations	2159:2209	effective, dynamical and dose-dependent regulations on intestinal microenvironment	2159:2240	CONCLUSION CCP can provide effective, dynamical and dose-dependent regulations on intestinal microenvironment, not only the intestinal flora but also the PPs and intestinal epithelium related immune response.
33152428	12	37	theme	intestinal	2294:2303	arg1	epithelium					2305:2314	intestinal epithelium	2294:2314	intestinal epithelium	2294:2314	CONCLUSION CCP can provide effective, dynamical and dose-dependent regulations on intestinal microenvironment, not only the intestinal flora but also the PPs and intestinal epithelium related immune response.
33152428	5	38	theme	pharmacological	1056:1070	arg1	mechanism					1072:1080	the pharmacological mechanism	1052:1080	the pharmacological mechanism of Coptis chinensis Franch	1052:1107	AIM OF THE STUDY This study intended to explore the dynamic regulation of CCP on intestinal microenvironment from the perspective of the intestinal mucosal immunity and the intestinal flora, in order to provide a new research perspective for the pharmacological mechanism of Coptis chinensis Franch.
33152428	13	39	theme	biological	2379:2388	arg1	activities					2390:2399	the multiple biological activities	2366:2399	the multiple biological activities of Coptis chinensis Franch	2366:2426	These may be involved in the multiple biological activities of Coptis chinensis Franch.
33152428	2	40	theme	clinical	434:441	arg1	efficacies					443:452	the clinical efficacies	430:452	the clinical efficacies of Coptis chinensis Franch against intestinal diseases	430:507	The medical records in past thousands years have fully confirmed the clinical efficacies of Coptis chinensis Franch against intestinal diseases.
33152428	8	41	theme	interleukin-4	1498:1510	arg1	analysis					1460:1467	the immunohistochemical analysis	1436:1467	the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue	1436:1632	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	2	42	theme	medical	369:375	arg1	records					377:383	The medical records	365:383	The medical records in past thousands years	365:407	The medical records in past thousands years have fully confirmed the clinical efficacies of Coptis chinensis Franch against intestinal diseases.
33152428	5	43	theme	intestinal	891:900	arg1	microenvironment					902:917	intestinal microenvironment	891:917	intestinal microenvironment	891:917	AIM OF THE STUDY This study intended to explore the dynamic regulation of CCP on intestinal microenvironment from the perspective of the intestinal mucosal immunity and the intestinal flora, in order to provide a new research perspective for the pharmacological mechanism of Coptis chinensis Franch.
33152428	5	44	dep	intended	838:845	arg1	AIM					810:812	AIM	810:812	AIM OF THE STUDY	810:825	AIM OF THE STUDY This study intended to explore the dynamic regulation of CCP on intestinal microenvironment from the perspective of the intestinal mucosal immunity and the intestinal flora, in order to provide a new research perspective for the pharmacological mechanism of Coptis chinensis Franch.
33152428	0	45	theme	Franch	17:22	arg1	polysaccharides					24:38	Coptis chinensis Franch polysaccharides	0:38	Coptis chinensis Franch polysaccharides	0:38	Coptis chinensis Franch polysaccharides provide a dynamically regulation on intestinal microenvironment, based on the intestinal flora and mucosal immunity.
33152428	2	46	theme	thousands	393:401	arg1	years					403:407	past thousands years	388:407	past thousands years	388:407	The medical records in past thousands years have fully confirmed the clinical efficacies of Coptis chinensis Franch against intestinal diseases.
33152428	8	47	from	interleukin-4	1498:1510	arg1	PPs					1597:1599	PPs	1597:1599	PPs	1597:1599	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	8	47	from	interleukin-4	1498:1510	arg1	tissue					1627:1632	intestinal epithelial tissue	1605:1632	intestinal epithelial tissue	1605:1632	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	11	48	theme	intestinal	2114:2123	arg1	flora					2125:2129	intestinal flora	2114:2129	intestinal flora	2114:2129	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues are consistent with the variation tendency of intestinal flora.
33152428	5	49	theme	intestinal	983:992	arg1	flora					994:998	the intestinal flora	979:998	the intestinal flora	979:998	AIM OF THE STUDY This study intended to explore the dynamic regulation of CCP on intestinal microenvironment from the perspective of the intestinal mucosal immunity and the intestinal flora, in order to provide a new research perspective for the pharmacological mechanism of Coptis chinensis Franch.
33152428	7	50	from	influences	1252:1261	arg1	flora					1284:1288	intestinal flora	1273:1288	intestinal flora	1273:1288	The influences of CCP on intestinal flora were evaluated by the 16sRNA gene illumina-miseq sequencing after gavage.
33152428	11	51	theme	variation	2092:2100	arg1	tendency					2102:2109	the variation tendency	2088:2109	the variation tendency of intestinal flora	2088:2129	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues are consistent with the variation tendency of intestinal flora.
33152428	4	52	theme	intestinal	781:790	arg1	microenvironment					792:807	intestinal microenvironment	781:807	intestinal microenvironment	781:807	It can be reasonably presumed that the polysaccharides in Coptis chinensis Franch (CCP) should be one of the critical element in the regulation of intestinal microenvironment.
33152428	9	53	with	RESULTS	1635:1641	arg1	CCP					1696:1698	CCP	1696:1698	CCP	1696:1698	RESULTS With the self-aggregation into particles morphology, CCP can be up-taken by PPs and promote the IFN-γ, IL-4, IL-17 and TGF-β secretion in PPs in a dose-dependent manner.
33152428	9	53	with	RESULTS	1635:1641	arg1	morphology					1684:1693	the self-aggregation into particles morphology	1648:1693	the self-aggregation into particles morphology	1648:1693	RESULTS With the self-aggregation into particles morphology, CCP can be up-taken by PPs and promote the IFN-γ, IL-4, IL-17 and TGF-β secretion in PPs in a dose-dependent manner.
33152428	0	54	theme	dynamically	50:60	arg1	regulation					62:71	a dynamically regulation	48:71	a dynamically regulation	48:71	Coptis chinensis Franch polysaccharides provide a dynamically regulation on intestinal microenvironment, based on the intestinal flora and mucosal immunity.
33152428	1	55	theme	Classic	318:324	arg1	"					343:343	"Shennong's Classic of Materia Medica"	306:343	"Shennong's Classic of Materia Medica" in the Han Dynasty	306:362	ETHNOPHARMACOLOGICAL RELEVANCE Coptis chinensis Franch is one of the most widely used traditional Chinese herbs in China and was firstly recorded in "Shennong's Classic of Materia Medica" in the Han Dynasty.
33152428	12	56	theme	intestinal	2214:2223	arg1	microenvironment					2225:2240	intestinal microenvironment	2214:2240	intestinal microenvironment	2214:2240	CONCLUSION CCP can provide effective, dynamical and dose-dependent regulations on intestinal microenvironment, not only the intestinal flora but also the PPs and intestinal epithelium related immune response.
33152428	12	57	from	epithelium	2305:2314	arg1	microenvironment					2225:2240	intestinal microenvironment	2214:2240	intestinal microenvironment	2214:2240	CONCLUSION CCP can provide effective, dynamical and dose-dependent regulations on intestinal microenvironment, not only the intestinal flora but also the PPs and intestinal epithelium related immune response.
33152428	4	58	theme	critical	743:750	arg1	element					752:758	the critical element	739:758	the critical element	739:758	It can be reasonably presumed that the polysaccharides in Coptis chinensis Franch (CCP) should be one of the critical element in the regulation of intestinal microenvironment.
33152428	1	59	theme	used	238:241	arg1	herbs					263:267	the most widely used traditional Chinese herbs	222:267	the most widely used traditional Chinese herbs in China	222:276	ETHNOPHARMACOLOGICAL RELEVANCE Coptis chinensis Franch is one of the most widely used traditional Chinese herbs in China and was firstly recorded in "Shennong's Classic of Materia Medica" in the Han Dynasty.
33152428	5	60	theme	mucosal	958:964	arg1	immunity					966:973	the intestinal mucosal immunity	943:973	the intestinal mucosal immunity	943:973	AIM OF THE STUDY This study intended to explore the dynamic regulation of CCP on intestinal microenvironment from the perspective of the intestinal mucosal immunity and the intestinal flora, in order to provide a new research perspective for the pharmacological mechanism of Coptis chinensis Franch.
33152428	8	61	theme	transforming	1547:1558	arg1	secretion					1584:1592	transforming growth factor-β (TGF-β) secretion	1547:1592	transforming growth factor-β (TGF-β) secretion	1547:1592	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	8	62	theme	mucosal	1401:1407	arg1	immunity					1409:1416	intestinal mucosal immunity	1390:1416	intestinal mucosal immunity	1390:1416	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	8	63	theme	intestinal	1605:1614	arg1	tissue					1627:1632	intestinal epithelial tissue	1605:1632	intestinal epithelial tissue	1605:1632	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	1	64	theme	Chinese	255:261	arg1	herbs					263:267	the most widely used traditional Chinese herbs	222:267	the most widely used traditional Chinese herbs in China	222:276	ETHNOPHARMACOLOGICAL RELEVANCE Coptis chinensis Franch is one of the most widely used traditional Chinese herbs in China and was firstly recorded in "Shennong's Classic of Materia Medica" in the Han Dynasty.
33152428	9	65	from	secretion	1768:1776	arg1	PPs					1781:1783	PPs	1781:1783	PPs	1781:1783	RESULTS With the self-aggregation into particles morphology, CCP can be up-taken by PPs and promote the IFN-γ, IL-4, IL-17 and TGF-β secretion in PPs in a dose-dependent manner.
33152428	8	66	theme	factor-β	1567:1574	arg1	secretion					1584:1592	transforming growth factor-β (TGF-β) secretion	1547:1592	transforming growth factor-β (TGF-β) secretion	1547:1592	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	8	67	from	interleukin-17	1520:1533	arg1	PPs					1597:1599	PPs	1597:1599	PPs	1597:1599	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	8	67	from	interleukin-17	1520:1533	arg1	tissue					1627:1632	intestinal epithelial tissue	1605:1632	intestinal epithelial tissue	1605:1632	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	8	68	theme	TGF-β	1577:1581	arg1	secretion					1584:1592	transforming growth factor-β (TGF-β) secretion	1547:1592	transforming growth factor-β (TGF-β) secretion	1547:1592	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	6	69	theme	CCP	1243:1245	arg1	perfusion					1217:1225	the perfusion	1213:1225	the perfusion of FITC labeled CCP	1213:1245	MATERIALS AND METHODS The absorption and distribution of CCP in intestinal tissues were observed after the perfusion of FITC labeled CCP.
33152428	8	70	theme	secretion	1584:1592	arg1	analysis					1460:1467	the immunohistochemical analysis	1436:1467	the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue	1436:1632	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	10	71	theme	intestinal	1849:1858	arg1	flora					1860:1864	the intestinal flora	1845:1864	the intestinal flora	1845:1864	The CCP can also be utilized by the intestinal flora and dynamically regulate the diversity, composition and distribution of the intestinal flora.
33152428	6	72	theme	FITC	1230:1233	arg1	CCP					1243:1245	FITC labeled CCP	1230:1245	FITC labeled CCP	1230:1245	MATERIALS AND METHODS The absorption and distribution of CCP in intestinal tissues were observed after the perfusion of FITC labeled CCP.
33152428	3	73	theme	herbal	533:538	arg1	medicines					540:548	herbal medicines	533:548	herbal medicines	533:548	The polysaccharides in herbal medicines can be digested by the flora and uptaken by the Peyer's patches (PPs) in intestine.
33152428	8	74	from	regulations	1368:1378	arg1	immunity					1409:1416	intestinal mucosal immunity	1390:1416	intestinal mucosal immunity	1390:1416	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	4	75	theme	microenvironment	792:807	arg1	regulation					767:776	the regulation	763:776	the regulation of intestinal microenvironment	763:807	It can be reasonably presumed that the polysaccharides in Coptis chinensis Franch (CCP) should be one of the critical element in the regulation of intestinal microenvironment.
33152428	11	76	theme	intestinal	2038:2047	arg1	tissues					2060:2066	intestinal epithelial tissues	2038:2066	intestinal epithelial tissues	2038:2066	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues are consistent with the variation tendency of intestinal flora.
33152428	11	77	with	consistent	2072:2081	arg1	tendency					2102:2109	the variation tendency	2088:2109	the variation tendency of intestinal flora	2088:2129	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues are consistent with the variation tendency of intestinal flora.
33152428	13	78	theme	Franch	2421:2426	arg1	activities					2390:2399	the multiple biological activities	2366:2399	the multiple biological activities of Coptis chinensis Franch	2366:2426	These may be involved in the multiple biological activities of Coptis chinensis Franch.
33152428	9	79	theme	self-aggregation	1652:1667	arg1	CCP					1696:1698	CCP	1696:1698	CCP	1696:1698	RESULTS With the self-aggregation into particles morphology, CCP can be up-taken by PPs and promote the IFN-γ, IL-4, IL-17 and TGF-β secretion in PPs in a dose-dependent manner.
33152428	9	79	theme	self-aggregation	1652:1667	arg1	morphology					1684:1693	the self-aggregation into particles morphology	1648:1693	the self-aggregation into particles morphology	1648:1693	RESULTS With the self-aggregation into particles morphology, CCP can be up-taken by PPs and promote the IFN-γ, IL-4, IL-17 and TGF-β secretion in PPs in a dose-dependent manner.
33152428	3	80	dep	flora	573:577	arg1	the					569:571	the	569:571	the	569:571	The polysaccharides in herbal medicines can be digested by the flora and uptaken by the Peyer's patches (PPs) in intestine.
33152428	5	81	theme	new	1023:1025	arg1	perspective					1036:1046	a new research perspective	1021:1046	a new research perspective for the pharmacological mechanism of Coptis chinensis Franch	1021:1107	AIM OF THE STUDY This study intended to explore the dynamic regulation of CCP on intestinal microenvironment from the perspective of the intestinal mucosal immunity and the intestinal flora, in order to provide a new research perspective for the pharmacological mechanism of Coptis chinensis Franch.
33152428	12	82	dep	PPs	2286:2288	arg1	response					2331:2338	related immune response	2316:2338	related immune response	2316:2338	CONCLUSION CCP can provide effective, dynamical and dose-dependent regulations on intestinal microenvironment, not only the intestinal flora but also the PPs and intestinal epithelium related immune response.
33152428	12	82	dep	PPs	2286:2288	arg1	the					2282:2284	the	2282:2284	the	2282:2284	CONCLUSION CCP can provide effective, dynamical and dose-dependent regulations on intestinal microenvironment, not only the intestinal flora but also the PPs and intestinal epithelium related immune response.
33152428	5	83	theme	dynamic	862:868	arg1	regulation					870:879	the dynamic regulation	858:879	the dynamic regulation of CCP on intestinal microenvironment	858:917	AIM OF THE STUDY This study intended to explore the dynamic regulation of CCP on intestinal microenvironment from the perspective of the intestinal mucosal immunity and the intestinal flora, in order to provide a new research perspective for the pharmacological mechanism of Coptis chinensis Franch.
33152428	8	84	theme	immunohistochemical	1440:1458	arg1	analysis					1460:1467	the immunohistochemical analysis	1436:1467	the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue	1436:1632	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	11	85	from	secretions	2024:2033	arg1	tissues					2060:2066	intestinal epithelial tissues	2038:2066	intestinal epithelial tissues	2038:2066	The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues are consistent with the variation tendency of intestinal flora.
33152428	12	86	theme	intestinal	2256:2265	arg1	flora					2267:2271	the intestinal flora	2252:2271	the intestinal flora	2252:2271	CONCLUSION CCP can provide effective, dynamical and dose-dependent regulations on intestinal microenvironment, not only the intestinal flora but also the PPs and intestinal epithelium related immune response.
33152428	7	87	theme	gene	1319:1322	arg1	sequencing					1339:1348	the 16sRNA gene illumina-miseq sequencing	1308:1348	the 16sRNA gene illumina-miseq sequencing	1308:1348	The influences of CCP on intestinal flora were evaluated by the 16sRNA gene illumina-miseq sequencing after gavage.
33152428	0	88	theme	intestinal	76:85	arg1	microenvironment					87:102	intestinal microenvironment	76:102	intestinal microenvironment	76:102	Coptis chinensis Franch polysaccharides provide a dynamically regulation on intestinal microenvironment, based on the intestinal flora and mucosal immunity.
33152428	6	89	from	distribution	1151:1162	arg1	tissues					1185:1191	intestinal tissues	1174:1191	intestinal tissues	1174:1191	MATERIALS AND METHODS The absorption and distribution of CCP in intestinal tissues were observed after the perfusion of FITC labeled CCP.
33152428	2	90	theme	intestinal	489:498	arg1	diseases					500:507	intestinal diseases	489:507	intestinal diseases	489:507	The medical records in past thousands years have fully confirmed the clinical efficacies of Coptis chinensis Franch against intestinal diseases.
33152428	8	91	theme	interferon-γ	1476:1487	arg1	analysis					1460:1467	the immunohistochemical analysis	1436:1467	the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue	1436:1632	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	12	92	theme	immune	2324:2329	arg1	response					2331:2338	related immune response	2316:2338	related immune response	2316:2338	CONCLUSION CCP can provide effective, dynamical and dose-dependent regulations on intestinal microenvironment, not only the intestinal flora but also the PPs and intestinal epithelium related immune response.
33152428	1	93	theme	Medica	337:342	arg1	Classic					318:324	Classic	318:324	Classic	318:324	ETHNOPHARMACOLOGICAL RELEVANCE Coptis chinensis Franch is one of the most widely used traditional Chinese herbs in China and was firstly recorded in "Shennong's Classic of Materia Medica" in the Han Dynasty.
33152428	2	94	theme	Franch	474:479	arg1	efficacies					443:452	the clinical efficacies	430:452	the clinical efficacies of Coptis chinensis Franch against intestinal diseases	430:507	The medical records in past thousands years have fully confirmed the clinical efficacies of Coptis chinensis Franch against intestinal diseases.
33152428	12	95	from	regulations	2199:2209	arg1	microenvironment					2225:2240	intestinal microenvironment	2214:2240	intestinal microenvironment	2214:2240	CONCLUSION CCP can provide effective, dynamical and dose-dependent regulations on intestinal microenvironment, not only the intestinal flora but also the PPs and intestinal epithelium related immune response.
33152428	5	96	theme	Franch	1102:1107	arg1	mechanism					1072:1080	the pharmacological mechanism	1052:1080	the pharmacological mechanism of Coptis chinensis Franch	1052:1107	AIM OF THE STUDY This study intended to explore the dynamic regulation of CCP on intestinal microenvironment from the perspective of the intestinal mucosal immunity and the intestinal flora, in order to provide a new research perspective for the pharmacological mechanism of Coptis chinensis Franch.
33152428	4	97	from	one	732:734	arg1	regulation					767:776	the regulation	763:776	the regulation of intestinal microenvironment	763:807	It can be reasonably presumed that the polysaccharides in Coptis chinensis Franch (CCP) should be one of the critical element in the regulation of intestinal microenvironment.
33152428	0	98	from	regulation	62:71	arg1	microenvironment					87:102	intestinal microenvironment	76:102	intestinal microenvironment	76:102	Coptis chinensis Franch polysaccharides provide a dynamically regulation on intestinal microenvironment, based on the intestinal flora and mucosal immunity.
33152428	6	99	from	absorption	1136:1145	arg1	tissues					1185:1191	intestinal tissues	1174:1191	intestinal tissues	1174:1191	MATERIALS AND METHODS The absorption and distribution of CCP in intestinal tissues were observed after the perfusion of FITC labeled CCP.
33152428	10	100	theme	flora	1953:1957	arg1	distribution					1922:1933	distribution	1922:1933	distribution	1922:1933	The CCP can also be utilized by the intestinal flora and dynamically regulate the diversity, composition and distribution of the intestinal flora.
33152428	10	100	theme	flora	1953:1957	arg1	composition					1906:1916	composition	1906:1916	composition	1906:1916	The CCP can also be utilized by the intestinal flora and dynamically regulate the diversity, composition and distribution of the intestinal flora.
33152428	10	100	theme	flora	1953:1957	arg1	diversity					1895:1903	diversity	1895:1903	diversity	1895:1903	The CCP can also be utilized by the intestinal flora and dynamically regulate the diversity, composition and distribution of the intestinal flora.
33152428	0	101	theme	intestinal	118:127	arg1	flora					129:133	intestinal flora	118:133	intestinal flora	118:133	Coptis chinensis Franch polysaccharides provide a dynamically regulation on intestinal microenvironment, based on the intestinal flora and mucosal immunity.
33152428	1	102	theme	Han	352:354	arg1	Dynasty					356:362	the Han Dynasty	348:362	the Han Dynasty	348:362	ETHNOPHARMACOLOGICAL RELEVANCE Coptis chinensis Franch is one of the most widely used traditional Chinese herbs in China and was firstly recorded in "Shennong's Classic of Materia Medica" in the Han Dynasty.
33152428	13	103	theme	multiple	2370:2377	arg1	activities					2390:2399	the multiple biological activities	2366:2399	the multiple biological activities of Coptis chinensis Franch	2366:2426	These may be involved in the multiple biological activities of Coptis chinensis Franch.
33152428	8	104	from	PPs	1597:1599	arg1	analysis					1460:1467	the immunohistochemical analysis	1436:1467	the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue	1436:1632	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	3	105	from	patches	606:612	arg1	intestine					623:631	intestine	623:631	intestine	623:631	The polysaccharides in herbal medicines can be digested by the flora and uptaken by the Peyer's patches (PPs) in intestine.
33152428	7	106	theme	intestinal	1273:1282	arg1	flora					1284:1288	intestinal flora	1273:1288	intestinal flora	1273:1288	The influences of CCP on intestinal flora were evaluated by the 16sRNA gene illumina-miseq sequencing after gavage.
33152428	8	107	from	interferon-γ	1476:1487	arg1	PPs					1597:1599	PPs	1597:1599	PPs	1597:1599	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	8	107	from	interferon-γ	1476:1487	arg1	tissue					1627:1632	intestinal epithelial tissue	1605:1632	intestinal epithelial tissue	1605:1632	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	5	108	from	regulation	870:879	arg1	microenvironment					902:917	intestinal microenvironment	891:917	intestinal microenvironment	891:917	AIM OF THE STUDY This study intended to explore the dynamic regulation of CCP on intestinal microenvironment from the perspective of the intestinal mucosal immunity and the intestinal flora, in order to provide a new research perspective for the pharmacological mechanism of Coptis chinensis Franch.
33152428	7	109	theme	CCP	1266:1268	arg1	influences					1252:1261	The influences	1248:1261	The influences of CCP on intestinal flora	1248:1288	The influences of CCP on intestinal flora were evaluated by the 16sRNA gene illumina-miseq sequencing after gavage.
33152428	8	110	theme	interleukin-17	1520:1533	arg1	analysis					1460:1467	the immunohistochemical analysis	1436:1467	the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue	1436:1632	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	8	111	from	tissue	1627:1632	arg1	analysis					1460:1467	the immunohistochemical analysis	1436:1467	the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue	1436:1632	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	1	112	from	"	343:343	arg1	Dynasty					356:362	the Han Dynasty	348:362	the Han Dynasty	348:362	ETHNOPHARMACOLOGICAL RELEVANCE Coptis chinensis Franch is one of the most widely used traditional Chinese herbs in China and was firstly recorded in "Shennong's Classic of Materia Medica" in the Han Dynasty.
33152428	9	113	from	IFN-γ	1739:1743	arg1	PPs					1781:1783	PPs	1781:1783	PPs	1781:1783	RESULTS With the self-aggregation into particles morphology, CCP can be up-taken by PPs and promote the IFN-γ, IL-4, IL-17 and TGF-β secretion in PPs in a dose-dependent manner.
33152428	4	114	from	polysaccharides	673:687	arg1	CCP					717:719	CCP	717:719	CCP	717:719	It can be reasonably presumed that the polysaccharides in Coptis chinensis Franch (CCP) should be one of the critical element in the regulation of intestinal microenvironment.
33152428	4	114	from	polysaccharides	673:687	arg1	Franch					709:714	Franch	709:714	Franch	709:714	It can be reasonably presumed that the polysaccharides in Coptis chinensis Franch (CCP) should be one of the critical element in the regulation of intestinal microenvironment.
33152428	9	115	theme	TGF-β	1762:1766	arg1	secretion					1768:1776	TGF-β secretion	1762:1776	TGF-β secretion	1762:1776	RESULTS With the self-aggregation into particles morphology, CCP can be up-taken by PPs and promote the IFN-γ, IL-4, IL-17 and TGF-β secretion in PPs in a dose-dependent manner.
33152428	2	116	theme	past	388:391	arg1	years					403:407	past thousands years	388:407	past thousands years	388:407	The medical records in past thousands years have fully confirmed the clinical efficacies of Coptis chinensis Franch against intestinal diseases.
33152428	12	117	theme	related	2316:2322	arg1	response					2331:2338	related immune response	2316:2338	related immune response	2316:2338	CONCLUSION CCP can provide effective, dynamical and dose-dependent regulations on intestinal microenvironment, not only the intestinal flora but also the PPs and intestinal epithelium related immune response.
33152428	4	118	theme	element	752:758	arg1	element					752:758	the critical element	739:758	the critical element	739:758	It can be reasonably presumed that the polysaccharides in Coptis chinensis Franch (CCP) should be one of the critical element in the regulation of intestinal microenvironment.
33152428	4	118	theme	element	752:758	arg1	one					732:734	one	732:734	one	732:734	It can be reasonably presumed that the polysaccharides in Coptis chinensis Franch (CCP) should be one of the critical element in the regulation of intestinal microenvironment.
33152428	4	118	theme	element	752:758	arg1	polysaccharides					673:687	the polysaccharides	669:687	the polysaccharides in Coptis chinensis Franch (CCP)	669:720	It can be reasonably presumed that the polysaccharides in Coptis chinensis Franch (CCP) should be one of the critical element in the regulation of intestinal microenvironment.
33152428	5	119	theme	intestinal	947:956	arg1	immunity					966:973	the intestinal mucosal immunity	943:973	the intestinal mucosal immunity	943:973	AIM OF THE STUDY This study intended to explore the dynamic regulation of CCP on intestinal microenvironment from the perspective of the intestinal mucosal immunity and the intestinal flora, in order to provide a new research perspective for the pharmacological mechanism of Coptis chinensis Franch.
33152428	2	120	from	records	377:383	arg1	years					403:407	past thousands years	388:407	past thousands years	388:407	The medical records in past thousands years have fully confirmed the clinical efficacies of Coptis chinensis Franch against intestinal diseases.
33152428	8	121	theme	growth	1560:1565	arg1	secretion					1584:1592	transforming growth factor-β (TGF-β) secretion	1547:1592	transforming growth factor-β (TGF-β) secretion	1547:1592	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	12	122	theme	effective	2159:2167	arg1	regulations					2199:2209	effective, dynamical and dose-dependent regulations	2159:2209	effective, dynamical and dose-dependent regulations on intestinal microenvironment	2159:2240	CONCLUSION CCP can provide effective, dynamical and dose-dependent regulations on intestinal microenvironment, not only the intestinal flora but also the PPs and intestinal epithelium related immune response.
33152428	8	123	theme	CCP	1383:1385	arg1	regulations					1368:1378	The regulations	1364:1378	The regulations of CCP on intestinal mucosal immunity	1364:1416	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	8	124	theme	epithelial	1616:1625	arg1	tissue					1627:1632	intestinal epithelial tissue	1605:1632	intestinal epithelial tissue	1605:1632	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	1	125	theme	traditional	243:253	arg1	herbs					263:267	the most widely used traditional Chinese herbs	222:267	the most widely used traditional Chinese herbs in China	222:276	ETHNOPHARMACOLOGICAL RELEVANCE Coptis chinensis Franch is one of the most widely used traditional Chinese herbs in China and was firstly recorded in "Shennong's Classic of Materia Medica" in the Han Dynasty.
33152428	5	126	theme	immunity	966:973	arg1	perspective					928:938	the perspective	924:938	the perspective of the intestinal mucosal immunity	924:973	AIM OF THE STUDY This study intended to explore the dynamic regulation of CCP on intestinal microenvironment from the perspective of the intestinal mucosal immunity and the intestinal flora, in order to provide a new research perspective for the pharmacological mechanism of Coptis chinensis Franch.
33152428	5	126	theme	immunity	966:973	arg1	flora					994:998	the intestinal flora	979:998	the intestinal flora	979:998	AIM OF THE STUDY This study intended to explore the dynamic regulation of CCP on intestinal microenvironment from the perspective of the intestinal mucosal immunity and the intestinal flora, in order to provide a new research perspective for the pharmacological mechanism of Coptis chinensis Franch.
33152428	9	127	theme	dose-dependent	1790:1803	arg1	manner					1805:1810	a dose-dependent manner	1788:1810	a dose-dependent manner	1788:1810	RESULTS With the self-aggregation into particles morphology, CCP can be up-taken by PPs and promote the IFN-γ, IL-4, IL-17 and TGF-β secretion in PPs in a dose-dependent manner.
33152428	8	128	theme	intestinal	1390:1399	arg1	immunity					1409:1416	intestinal mucosal immunity	1390:1416	intestinal mucosal immunity	1390:1416	The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue.
33152428	1	129	theme	herbs	263:267	arg1	one					215:217	one	215:217	one	215:217	ETHNOPHARMACOLOGICAL RELEVANCE Coptis chinensis Franch is one of the most widely used traditional Chinese herbs in China and was firstly recorded in "Shennong's Classic of Materia Medica" in the Han Dynasty.
33152428	1	129	theme	herbs	263:267	arg1	herbs					263:267	the most widely used traditional Chinese herbs	222:267	the most widely used traditional Chinese herbs in China	222:276	ETHNOPHARMACOLOGICAL RELEVANCE Coptis chinensis Franch is one of the most widely used traditional Chinese herbs in China and was firstly recorded in "Shennong's Classic of Materia Medica" in the Han Dynasty.
32294591	0	0	theme	electroactive	87:99	arg1	biofilm					101:107	electroactive biofilm	87:107	electroactive biofilm	87:107	Acetate limitation selects Geobacter from mixed inoculum and reduces polysaccharide in electroactive biofilm.
32294591	7	1	theme	selective	1121:1129	arg1	pressure					1131:1138	a selective pressure	1119:1138	a selective pressure to enrich Geobacter up to 88% compared to 72% when acetate was abundant	1119:1210	Limited acetate also imposed a selective pressure to enrich Geobacter up to 88% compared to 72% when acetate was abundant.
32294591	8	2	theme	potential	1423:1431	arg1	applications					1433:1444	potential applications	1423:1444	potential applications for bioenergy recovery and trace toxicant detection	1423:1496	Our findings demonstrate that a highly active EAB can be formed by limiting substrate concentration, providing a broader understanding of the EAB formation process, the ecology of interspecies competitions and potential applications for bioenergy recovery and trace toxicant detection in the future.
32294591	5	3	theme	current	848:854	arg1	density					856:862	a 90% higher current density	835:862	a 90% higher current density	835:862	The EAB formed with 0.1 g/L of acetate (EAB-0.1) exhibited a 90% higher current density than that formed with 1.0 g/L acetate (EAB-1.0).
32294591	6	4	theme	higher	931:936	arg1	electroactivity					938:952	a 50% higher electroactivity	925:952	a 50% higher electroactivity per biomass	925:964	EAB-0.1 had a 50% higher electroactivity per biomass and a 20% thinner thickness than EAB-1.0, which was partly due to the 54% decrease of insulative polysaccharide in biofilm.
32294591	8	5	theme	interspecies	1393:1404	arg1	competitions					1406:1417	interspecies competitions	1393:1417	interspecies competitions	1393:1417	Our findings demonstrate that a highly active EAB can be formed by limiting substrate concentration, providing a broader understanding of the EAB formation process, the ecology of interspecies competitions and potential applications for bioenergy recovery and trace toxicant detection in the future.
32294591	4	6	theme	BES	672:674	arg1	performance					657:667	the performance	653:667	the performance of BES	653:674	Here we confirmed that microbial community composition and the morphology of EAB on the electrode including the thickness and porosity of the biofilm are critical for the performance of BES, and these properties can be simply controlled by the substrate concentration during EAB formation.
32294591	4	7	theme	EAB	563:565	arg1	critical					640:647	critical	640:647	critical	640:647	Here we confirmed that microbial community composition and the morphology of EAB on the electrode including the thickness and porosity of the biofilm are critical for the performance of BES, and these properties can be simply controlled by the substrate concentration during EAB formation.
32294591	4	7	theme	EAB	563:565	arg1	composition					529:539	microbial community composition	509:539	microbial community composition	509:539	Here we confirmed that microbial community composition and the morphology of EAB on the electrode including the thickness and porosity of the biofilm are critical for the performance of BES, and these properties can be simply controlled by the substrate concentration during EAB formation.
32294591	4	7	theme	EAB	563:565	arg1	morphology					549:558	the morphology	545:558	the morphology of EAB on the electrode including the thickness and porosity of the biofilm	545:634	Here we confirmed that microbial community composition and the morphology of EAB on the electrode including the thickness and porosity of the biofilm are critical for the performance of BES, and these properties can be simply controlled by the substrate concentration during EAB formation.
32294591	4	8	theme	substrate	730:738	arg1	concentration					740:752	the substrate concentration	726:752	the substrate concentration during EAB formation	726:773	Here we confirmed that microbial community composition and the morphology of EAB on the electrode including the thickness and porosity of the biofilm are critical for the performance of BES, and these properties can be simply controlled by the substrate concentration during EAB formation.
32294591	3	9	theme	BES	362:364	arg1	core					354:357	the core	350:357	the core of BES	350:364	As the core of BES, it is still unclear how the EAB is formed from mixed inoculum, and how exoelectrogens compete with non-exoelectrogens.
32294591	0	10	from	inoculum	48:55	arg1	Geobacter					27:35	Geobacter	27:35	Geobacter from mixed inoculum	27:55	Acetate limitation selects Geobacter from mixed inoculum and reduces polysaccharide in electroactive biofilm.
32294591	3	11	dep	unclear	379:385	arg1	compete					453:459	compete	453:459	compete with non-exoelectrogens	453:483	As the core of BES, it is still unclear how the EAB is formed from mixed inoculum, and how exoelectrogens compete with non-exoelectrogens.
32294591	3	11	dep	unclear	379:385	arg1	formed					402:407	formed	402:407	is formed from mixed inoculum	399:427	As the core of BES, it is still unclear how the EAB is formed from mixed inoculum, and how exoelectrogens compete with non-exoelectrogens.
32294591	6	12	theme	%	974:974	arg1	thickness					984:992	a 20% thinner thickness	970:992	a 20% thinner thickness	970:992	EAB-0.1 had a 50% higher electroactivity per biomass and a 20% thinner thickness than EAB-1.0, which was partly due to the 54% decrease of insulative polysaccharide in biofilm.
32294591	1	13	theme	promising	173:181	arg1	technology					183:192	a promising technology	171:192	a promising technology to recover bioenergy or synthesize value-added products from wastewaters	171:265	Bioelectrochemical systems (BESs) are widely investigated as a promising technology to recover bioenergy or synthesize value-added products from wastewaters.
32294591	1	13	theme	promising	173:181	arg1	systems					129:135	Bioelectrochemical systems	110:135	Bioelectrochemical systems (BESs)	110:142	Bioelectrochemical systems (BESs) are widely investigated as a promising technology to recover bioenergy or synthesize value-added products from wastewaters.
32294591	4	14	from	composition	529:539	arg1	electrode					574:582	the electrode	570:582	the electrode including the thickness and porosity of the biofilm	570:634	Here we confirmed that microbial community composition and the morphology of EAB on the electrode including the thickness and porosity of the biofilm are critical for the performance of BES, and these properties can be simply controlled by the substrate concentration during EAB formation.
32294591	4	15	from	morphology	549:558	arg1	electrode					574:582	the electrode	570:582	the electrode including the thickness and porosity of the biofilm	570:634	Here we confirmed that microbial community composition and the morphology of EAB on the electrode including the thickness and porosity of the biofilm are critical for the performance of BES, and these properties can be simply controlled by the substrate concentration during EAB formation.
32294591	3	16	theme	mixed	414:418	arg1	inoculum					420:427	mixed inoculum	414:427	mixed inoculum	414:427	As the core of BES, it is still unclear how the EAB is formed from mixed inoculum, and how exoelectrogens compete with non-exoelectrogens.
32294591	6	17	theme	polysaccharide	1063:1076	arg1	decrease					1040:1047	the 54% decrease	1032:1047	the 54% decrease of insulative polysaccharide in biofilm	1032:1087	EAB-0.1 had a 50% higher electroactivity per biomass and a 20% thinner thickness than EAB-1.0, which was partly due to the 54% decrease of insulative polysaccharide in biofilm.
32294591	5	18	theme	1.0 g/L	886:892	arg1	EAB-1.0					903:909	EAB-1.0	903:909	EAB-1.0	903:909	The EAB formed with 0.1 g/L of acetate (EAB-0.1) exhibited a 90% higher current density than that formed with 1.0 g/L acetate (EAB-1.0).
32294591	5	18	theme	1.0 g/L	886:892	arg1	acetate					894:900	1.0 g/L acetate	886:900	1.0 g/L acetate (EAB-1.0)	886:910	The EAB formed with 0.1 g/L of acetate (EAB-0.1) exhibited a 90% higher current density than that formed with 1.0 g/L acetate (EAB-1.0).
32294591	8	19	theme	competitions	1406:1417	arg1	applications					1433:1444	potential applications	1423:1444	potential applications for bioenergy recovery and trace toxicant detection	1423:1496	Our findings demonstrate that a highly active EAB can be formed by limiting substrate concentration, providing a broader understanding of the EAB formation process, the ecology of interspecies competitions and potential applications for bioenergy recovery and trace toxicant detection in the future.
32294591	8	19	theme	competitions	1406:1417	arg1	understanding					1334:1346	a broader understanding	1324:1346	a broader understanding of the EAB formation process	1324:1375	Our findings demonstrate that a highly active EAB can be formed by limiting substrate concentration, providing a broader understanding of the EAB formation process, the ecology of interspecies competitions and potential applications for bioenergy recovery and trace toxicant detection in the future.
32294591	8	19	theme	competitions	1406:1417	arg1	ecology					1382:1388	the ecology	1378:1388	the ecology of interspecies competitions	1378:1417	Our findings demonstrate that a highly active EAB can be formed by limiting substrate concentration, providing a broader understanding of the EAB formation process, the ecology of interspecies competitions and potential applications for bioenergy recovery and trace toxicant detection in the future.
32294591	0	20	theme	Acetate	0:6	arg1	limitation					8:17	Acetate limitation	0:17	Acetate limitation	0:17	Acetate limitation selects Geobacter from mixed inoculum and reduces polysaccharide in electroactive biofilm.
32294591	8	21	theme	bioenergy	1450:1458	arg1	recovery					1460:1467	bioenergy recovery	1450:1467	bioenergy recovery	1450:1467	Our findings demonstrate that a highly active EAB can be formed by limiting substrate concentration, providing a broader understanding of the EAB formation process, the ecology of interspecies competitions and potential applications for bioenergy recovery and trace toxicant detection in the future.
32294591	2	22	theme	biofilm	332:338	arg1	activity					306:313	the activity	302:313	the activity of electroactive biofilm (EAB)	302:344	The performance of BES depends on the activity of electroactive biofilm (EAB).
32294591	8	23	theme	broader	1326:1332	arg1	understanding					1334:1346	a broader understanding	1324:1346	a broader understanding of the EAB formation process	1324:1375	Our findings demonstrate that a highly active EAB can be formed by limiting substrate concentration, providing a broader understanding of the EAB formation process, the ecology of interspecies competitions and potential applications for bioenergy recovery and trace toxicant detection in the future.
32294591	2	24	theme	electroactive	318:330	arg1	EAB					341:343	EAB	341:343	EAB	341:343	The performance of BES depends on the activity of electroactive biofilm (EAB).
32294591	2	24	theme	electroactive	318:330	arg1	biofilm					332:338	electroactive biofilm	318:338	electroactive biofilm (EAB)	318:344	The performance of BES depends on the activity of electroactive biofilm (EAB).
32294591	6	25	from	decrease	1040:1047	arg1	biofilm					1081:1087	biofilm	1081:1087	biofilm	1081:1087	EAB-0.1 had a 50% higher electroactivity per biomass and a 20% thinner thickness than EAB-1.0, which was partly due to the 54% decrease of insulative polysaccharide in biofilm.
32294591	6	26	theme	thinner	976:982	arg1	thickness					984:992	a 20% thinner thickness	970:992	a 20% thinner thickness	970:992	EAB-0.1 had a 50% higher electroactivity per biomass and a 20% thinner thickness than EAB-1.0, which was partly due to the 54% decrease of insulative polysaccharide in biofilm.
32294591	6	27	theme	insulative	1052:1061	arg1	polysaccharide					1063:1076	insulative polysaccharide	1052:1076	insulative polysaccharide	1052:1076	EAB-0.1 had a 50% higher electroactivity per biomass and a 20% thinner thickness than EAB-1.0, which was partly due to the 54% decrease of insulative polysaccharide in biofilm.
32294591	5	28	theme	acetate	807:813	arg1	0.1 g/L					796:802	0.1 g/L	796:802	0.1 g/L of acetate (EAB-0.1)	796:823	The EAB formed with 0.1 g/L of acetate (EAB-0.1) exhibited a 90% higher current density than that formed with 1.0 g/L acetate (EAB-1.0).
32294591	0	29	theme	mixed	42:46	arg1	inoculum					48:55	mixed inoculum	42:55	mixed inoculum	42:55	Acetate limitation selects Geobacter from mixed inoculum and reduces polysaccharide in electroactive biofilm.
32294591	6	30	theme	20	972:973	arg1	%					974:974	%	974:974	%	974:974	EAB-0.1 had a 50% higher electroactivity per biomass and a 20% thinner thickness than EAB-1.0, which was partly due to the 54% decrease of insulative polysaccharide in biofilm.
32294591	4	31	theme	biofilm	628:634	arg1	porosity					612:619	porosity	612:619	porosity	612:619	Here we confirmed that microbial community composition and the morphology of EAB on the electrode including the thickness and porosity of the biofilm are critical for the performance of BES, and these properties can be simply controlled by the substrate concentration during EAB formation.
32294591	4	31	theme	biofilm	628:634	arg1	thickness					598:606	thickness	598:606	thickness	598:606	Here we confirmed that microbial community composition and the morphology of EAB on the electrode including the thickness and porosity of the biofilm are critical for the performance of BES, and these properties can be simply controlled by the substrate concentration during EAB formation.
32294591	4	32	theme	microbial	509:517	arg1	composition					529:539	microbial community composition	509:539	microbial community composition	509:539	Here we confirmed that microbial community composition and the morphology of EAB on the electrode including the thickness and porosity of the biofilm are critical for the performance of BES, and these properties can be simply controlled by the substrate concentration during EAB formation.
32294591	0	33	from	polysaccharide	69:82	arg1	biofilm					101:107	electroactive biofilm	87:107	electroactive biofilm	87:107	Acetate limitation selects Geobacter from mixed inoculum and reduces polysaccharide in electroactive biofilm.
32294591	1	34	theme	value-added	229:239	arg1	products					241:248	value-added products	229:248	value-added products from wastewaters	229:265	Bioelectrochemical systems (BESs) are widely investigated as a promising technology to recover bioenergy or synthesize value-added products from wastewaters.
32294591	8	35	theme	trace	1473:1477	arg1	detection					1488:1496	trace toxicant detection	1473:1496	trace toxicant detection	1473:1496	Our findings demonstrate that a highly active EAB can be formed by limiting substrate concentration, providing a broader understanding of the EAB formation process, the ecology of interspecies competitions and potential applications for bioenergy recovery and trace toxicant detection in the future.
32294591	1	36	from	wastewaters	255:265	arg1	products					241:248	value-added products	229:248	value-added products from wastewaters	229:265	Bioelectrochemical systems (BESs) are widely investigated as a promising technology to recover bioenergy or synthesize value-added products from wastewaters.
32294591	8	37	theme	substrate	1289:1297	arg1	concentration					1299:1311	substrate concentration	1289:1311	substrate concentration	1289:1311	Our findings demonstrate that a highly active EAB can be formed by limiting substrate concentration, providing a broader understanding of the EAB formation process, the ecology of interspecies competitions and potential applications for bioenergy recovery and trace toxicant detection in the future.
32294591	8	38	theme	active	1252:1257	arg1	EAB					1259:1261	a highly active EAB	1243:1261	a highly active EAB	1243:1261	Our findings demonstrate that a highly active EAB can be formed by limiting substrate concentration, providing a broader understanding of the EAB formation process, the ecology of interspecies competitions and potential applications for bioenergy recovery and trace toxicant detection in the future.
32294591	6	39	theme	%	1038:1038	arg1	decrease					1040:1047	the 54% decrease	1032:1047	the 54% decrease of insulative polysaccharide in biofilm	1032:1087	EAB-0.1 had a 50% higher electroactivity per biomass and a 20% thinner thickness than EAB-1.0, which was partly due to the 54% decrease of insulative polysaccharide in biofilm.
32294591	8	40	theme	formation	1359:1367	arg1	process					1369:1375	the EAB formation process	1351:1375	the EAB formation process	1351:1375	Our findings demonstrate that a highly active EAB can be formed by limiting substrate concentration, providing a broader understanding of the EAB formation process, the ecology of interspecies competitions and potential applications for bioenergy recovery and trace toxicant detection in the future.
32294591	6	41	contain	had	921:923	arg1	EAB-0.1					913:919	EAB-0.1	913:919	EAB-0.1	913:919	EAB-0.1 had a 50% higher electroactivity per biomass and a 20% thinner thickness than EAB-1.0, which was partly due to the 54% decrease of insulative polysaccharide in biofilm.
32294591	6	41	contain	had	921:923	arg2	electroactivity					938:952	a 50% higher electroactivity	925:952	a 50% higher electroactivity per biomass	925:964	EAB-0.1 had a 50% higher electroactivity per biomass and a 20% thinner thickness than EAB-1.0, which was partly due to the 54% decrease of insulative polysaccharide in biofilm.
32294591	6	41	contain	had	921:923	arg2	thickness					984:992	a 20% thinner thickness	970:992	a 20% thinner thickness	970:992	EAB-0.1 had a 50% higher electroactivity per biomass and a 20% thinner thickness than EAB-1.0, which was partly due to the 54% decrease of insulative polysaccharide in biofilm.
32294591	6	42	theme	54	1036:1037	arg1	%					1038:1038	%	1038:1038	%	1038:1038	EAB-0.1 had a 50% higher electroactivity per biomass and a 20% thinner thickness than EAB-1.0, which was partly due to the 54% decrease of insulative polysaccharide in biofilm.
32294591	4	43	theme	community	519:527	arg1	composition					529:539	microbial community composition	509:539	microbial community composition	509:539	Here we confirmed that microbial community composition and the morphology of EAB on the electrode including the thickness and porosity of the biofilm are critical for the performance of BES, and these properties can be simply controlled by the substrate concentration during EAB formation.
32294591	4	44	dep	thickness	598:606	arg1	the					594:596	the	594:596	the	594:596	Here we confirmed that microbial community composition and the morphology of EAB on the electrode including the thickness and porosity of the biofilm are critical for the performance of BES, and these properties can be simply controlled by the substrate concentration during EAB formation.
32294591	2	45	theme	BES	287:289	arg1	performance					272:282	The performance	268:282	The performance of BES	268:289	The performance of BES depends on the activity of electroactive biofilm (EAB).
32294591	1	46	theme	Bioelectrochemical	110:127	arg1	technology					183:192	a promising technology	171:192	a promising technology to recover bioenergy or synthesize value-added products from wastewaters	171:265	Bioelectrochemical systems (BESs) are widely investigated as a promising technology to recover bioenergy or synthesize value-added products from wastewaters.
32294591	1	46	theme	Bioelectrochemical	110:127	arg1	BESs					138:141	BESs	138:141	BESs	138:141	Bioelectrochemical systems (BESs) are widely investigated as a promising technology to recover bioenergy or synthesize value-added products from wastewaters.
32294591	1	46	theme	Bioelectrochemical	110:127	arg1	systems					129:135	Bioelectrochemical systems	110:135	Bioelectrochemical systems (BESs)	110:142	Bioelectrochemical systems (BESs) are widely investigated as a promising technology to recover bioenergy or synthesize value-added products from wastewaters.
32294591	4	47	theme	EAB	761:763	arg1	formation					765:773	EAB formation	761:773	EAB formation	761:773	Here we confirmed that microbial community composition and the morphology of EAB on the electrode including the thickness and porosity of the biofilm are critical for the performance of BES, and these properties can be simply controlled by the substrate concentration during EAB formation.
32294591	8	48	theme	EAB	1355:1357	arg1	process					1369:1375	the EAB formation process	1351:1375	the EAB formation process	1351:1375	Our findings demonstrate that a highly active EAB can be formed by limiting substrate concentration, providing a broader understanding of the EAB formation process, the ecology of interspecies competitions and potential applications for bioenergy recovery and trace toxicant detection in the future.
32294591	8	49	theme	process	1369:1375	arg1	applications					1433:1444	potential applications	1423:1444	potential applications for bioenergy recovery and trace toxicant detection	1423:1496	Our findings demonstrate that a highly active EAB can be formed by limiting substrate concentration, providing a broader understanding of the EAB formation process, the ecology of interspecies competitions and potential applications for bioenergy recovery and trace toxicant detection in the future.
32294591	8	49	theme	process	1369:1375	arg1	understanding					1334:1346	a broader understanding	1324:1346	a broader understanding of the EAB formation process	1324:1375	Our findings demonstrate that a highly active EAB can be formed by limiting substrate concentration, providing a broader understanding of the EAB formation process, the ecology of interspecies competitions and potential applications for bioenergy recovery and trace toxicant detection in the future.
32294591	8	49	theme	process	1369:1375	arg1	ecology					1382:1388	the ecology	1378:1388	the ecology of interspecies competitions	1378:1417	Our findings demonstrate that a highly active EAB can be formed by limiting substrate concentration, providing a broader understanding of the EAB formation process, the ecology of interspecies competitions and potential applications for bioenergy recovery and trace toxicant detection in the future.
32294591	8	50	theme	toxicant	1479:1486	arg1	detection					1488:1496	trace toxicant detection	1473:1496	trace toxicant detection	1473:1496	Our findings demonstrate that a highly active EAB can be formed by limiting substrate concentration, providing a broader understanding of the EAB formation process, the ecology of interspecies competitions and potential applications for bioenergy recovery and trace toxicant detection in the future.
32294591	5	51	theme	higher	841:846	arg1	density					856:862	a 90% higher current density	835:862	a 90% higher current density	835:862	The EAB formed with 0.1 g/L of acetate (EAB-0.1) exhibited a 90% higher current density than that formed with 1.0 g/L acetate (EAB-1.0).
32294591	7	52	theme	Limited	1090:1096	arg1	acetate					1098:1104	Limited acetate	1090:1104	Limited acetate	1090:1104	Limited acetate also imposed a selective pressure to enrich Geobacter up to 88% compared to 72% when acetate was abundant.
34474878	10	0	theme	Antimicrobial	1310:1322	arg1	tests					1324:1328	Antimicrobial tests	1310:1328	Antimicrobial tests	1310:1328	Antimicrobial tests indicated the COS/PMarg cryogel could effectively inhibit the proliferation of Candida albicans.
34474878	6	1	with	microscope	876:885	arg1	size					903:906	pore size varying	898:914	pore size varying from 20.86 to 50.87 μm	898:937	The macroporous morphology of the cryogels was confirmed by scanning electron microscope (SEM) with pore size varying from 20.86 to 50.87 μm.
34474878	11	2	theme	potential	1516:1524	arg1	cryogel					1497:1503	the macroporous zwitterionic COS/PMarg composite cryogel	1448:1503	the macroporous zwitterionic COS/PMarg composite cryogel	1448:1503	It demonstrated that the macroporous zwitterionic COS/PMarg composite cryogel might be a potential antifungal dressing with sequential "sterilization-release" capacity.
34474878	11	2	theme	potential	1516:1524	arg1	dressing					1537:1544	a potential antifungal dressing	1514:1544	a potential antifungal dressing with sequential "sterilization-release" capacity	1514:1593	It demonstrated that the macroporous zwitterionic COS/PMarg composite cryogel might be a potential antifungal dressing with sequential "sterilization-release" capacity.
34474878	5	3	theme	COS/PMarg	725:733	arg1	cryogels					735:742	the COS/PMarg cryogels	721:742	the COS/PMarg cryogels	721:742	Then, the COS/PMarg cryogels were prepared by redox initiation cryopolymerization.
34474878	9	4	theme	COS	1254:1256	arg1	release					1243:1249	the release	1239:1249	the release of COS	1239:1256	With the release of COS, the antifouling activity of the cryogel increased.
34474878	7	5	theme	thermal-gravimetric	1086:1104	arg1	TGA					1116:1118	TGA	1116:1118	TGA	1116:1118	FTIR indicated that hydrogen bonding formed between COS and PMarg, and the interaction elevated thermal stability of the cryogels as evidenced by thermal-gravimetric analysis (TGA).
34474878	7	5	theme	thermal-gravimetric	1086:1104	arg1	analysis					1106:1113	thermal-gravimetric analysis	1086:1113	thermal-gravimetric analysis (TGA)	1086:1119	FTIR indicated that hydrogen bonding formed between COS and PMarg, and the interaction elevated thermal stability of the cryogels as evidenced by thermal-gravimetric analysis (TGA).
34474878	11	6	theme	COS/PMarg	1477:1485	arg1	cryogel					1497:1503	the macroporous zwitterionic COS/PMarg composite cryogel	1448:1503	the macroporous zwitterionic COS/PMarg composite cryogel	1448:1503	It demonstrated that the macroporous zwitterionic COS/PMarg composite cryogel might be a potential antifungal dressing with sequential "sterilization-release" capacity.
34474878	11	6	theme	COS/PMarg	1477:1485	arg1	dressing					1537:1544	a potential antifungal dressing	1514:1544	a potential antifungal dressing with sequential "sterilization-release" capacity	1514:1593	It demonstrated that the macroporous zwitterionic COS/PMarg composite cryogel might be a potential antifungal dressing with sequential "sterilization-release" capacity.
34474878	8	7	theme	Swelling	1122:1129	arg1	capacity					1131:1138	Swelling capacity	1122:1138	Swelling capacity	1122:1138	Swelling capacity, mechanical properties, and COS release behavior of the COS/PMarg cryogels were investigated.
34474878	10	8	theme	albicans	1417:1424	arg1	proliferation					1392:1404	the proliferation	1388:1404	the proliferation of Candida albicans	1388:1424	Antimicrobial tests indicated the COS/PMarg cryogel could effectively inhibit the proliferation of Candida albicans.
34474878	11	9	theme	sequential	1551:1560	arg1	"					1584:1584	sequential "sterilization-release"	1551:1584	sequential "sterilization-release" capacity	1551:1593	It demonstrated that the macroporous zwitterionic COS/PMarg composite cryogel might be a potential antifungal dressing with sequential "sterilization-release" capacity.
34474878	10	10	theme	Candida	1409:1415	arg1	albicans					1417:1424	Candida albicans	1409:1424	Candida albicans	1409:1424	Antimicrobial tests indicated the COS/PMarg cryogel could effectively inhibit the proliferation of Candida albicans.
34474878	11	11	theme	antifungal	1526:1535	arg1	cryogel					1497:1503	the macroporous zwitterionic COS/PMarg composite cryogel	1448:1503	the macroporous zwitterionic COS/PMarg composite cryogel	1448:1503	It demonstrated that the macroporous zwitterionic COS/PMarg composite cryogel might be a potential antifungal dressing with sequential "sterilization-release" capacity.
34474878	11	11	theme	antifungal	1526:1535	arg1	dressing					1537:1544	a potential antifungal dressing	1514:1544	a potential antifungal dressing with sequential "sterilization-release" capacity	1514:1593	It demonstrated that the macroporous zwitterionic COS/PMarg composite cryogel might be a potential antifungal dressing with sequential "sterilization-release" capacity.
34474878	4	12	theme	mass	689:692	arg1	HRMS					708:711	HRMS	708:711	HRMS	708:711	Firstly, Marg was synthesized and characterized by Fourier transform infrared spectroscopy (FT-IR), 1H and 13C nuclear magnetic resonance (NMR), and high-resolution mass spectrometry (HRMS).
34474878	4	12	theme	mass	689:692	arg1	spectrometry					694:705	high-resolution mass spectrometry	673:705	high-resolution mass spectrometry (HRMS)	673:712	Firstly, Marg was synthesized and characterized by Fourier transform infrared spectroscopy (FT-IR), 1H and 13C nuclear magnetic resonance (NMR), and high-resolution mass spectrometry (HRMS).
34474878	6	13	theme	electron	867:874	arg1	SEM					888:890	SEM	888:890	SEM	888:890	The macroporous morphology of the cryogels was confirmed by scanning electron microscope (SEM) with pore size varying from 20.86 to 50.87 μm.
34474878	6	13	theme	electron	867:874	arg1	microscope					876:885	scanning electron microscope	858:885	scanning electron microscope (SEM) with pore size varying from 20.86 to 50.87 μm	858:937	The macroporous morphology of the cryogels was confirmed by scanning electron microscope (SEM) with pore size varying from 20.86 to 50.87 μm.
34474878	5	14	theme	redox	761:765	arg1	cryopolymerization					778:795	redox initiation cryopolymerization	761:795	redox initiation cryopolymerization	761:795	Then, the COS/PMarg cryogels were prepared by redox initiation cryopolymerization.
34474878	4	15	theme	nuclear	635:641	arg1	NMR					663:665	NMR	663:665	NMR	663:665	Firstly, Marg was synthesized and characterized by Fourier transform infrared spectroscopy (FT-IR), 1H and 13C nuclear magnetic resonance (NMR), and high-resolution mass spectrometry (HRMS).
34474878	4	15	theme	nuclear	635:641	arg1	resonance					652:660	nuclear magnetic resonance	635:660	nuclear magnetic resonance (NMR)	635:666	Firstly, Marg was synthesized and characterized by Fourier transform infrared spectroscopy (FT-IR), 1H and 13C nuclear magnetic resonance (NMR), and high-resolution mass spectrometry (HRMS).
34474878	6	16	theme	scanning	858:865	arg1	SEM					888:890	SEM	888:890	SEM	888:890	The macroporous morphology of the cryogels was confirmed by scanning electron microscope (SEM) with pore size varying from 20.86 to 50.87 μm.
34474878	6	16	theme	scanning	858:865	arg1	microscope					876:885	scanning electron microscope	858:885	scanning electron microscope (SEM) with pore size varying from 20.86 to 50.87 μm	858:937	The macroporous morphology of the cryogels was confirmed by scanning electron microscope (SEM) with pore size varying from 20.86 to 50.87 μm.
34474878	7	17	theme	cryogels	1061:1068	arg1	stability					1044:1052	thermal stability	1036:1052	thermal stability of the cryogels	1036:1068	FTIR indicated that hydrogen bonding formed between COS and PMarg, and the interaction elevated thermal stability of the cryogels as evidenced by thermal-gravimetric analysis (TGA).
34474878	11	18	theme	"	1584:1584	arg1	capacity					1586:1593	sequential "sterilization-release" capacity	1551:1593	sequential "sterilization-release" capacity	1551:1593	It demonstrated that the macroporous zwitterionic COS/PMarg composite cryogel might be a potential antifungal dressing with sequential "sterilization-release" capacity.
34474878	3	19	theme	composite	390:398	arg1	cryogels					400:407	macroporous zwitterionic composite cryogels	365:407	macroporous zwitterionic composite cryogels composed of COS and poly(N-methacryl arginine) (PMarg)	365:462	In this research, macroporous zwitterionic composite cryogels composed of COS and poly(N-methacryl arginine) (PMarg) were fabricated, serving as long-term antifungal dressings.
34474878	3	20	theme	N-methacryl	434:444	arg1	poly					429:432	poly	429:432	poly(N-methacryl arginine) (PMarg)	429:462	In this research, macroporous zwitterionic composite cryogels composed of COS and poly(N-methacryl arginine) (PMarg) were fabricated, serving as long-term antifungal dressings.
34474878	3	20	theme	N-methacryl	434:444	arg1	arginine					446:453	N-methacryl arginine	434:453	N-methacryl arginine	434:453	In this research, macroporous zwitterionic composite cryogels composed of COS and poly(N-methacryl arginine) (PMarg) were fabricated, serving as long-term antifungal dressings.
34474878	0	21	theme	zwitterionic	12:23	arg1	cryogel					35:41	Macroporous zwitterionic composite cryogel	0:41	Macroporous zwitterionic composite cryogel	0:41	Macroporous zwitterionic composite cryogel based on chitosan oligosaccharide for antifungal application.
34474878	1	22	theme	Chitosan	105:112	arg1	oligosaccharide					114:128	Chitosan oligosaccharide	105:128	Chitosan oligosaccharide (COS)	105:134	Chitosan oligosaccharide (COS), a time-dependent antimicrobial carbohydrate, is found antifungal active with a short duration of action due to excessive solubility.
34474878	1	22	theme	Chitosan	105:112	arg1	carbohydrate					168:179	a time-dependent antimicrobial carbohydrate	137:179	a time-dependent antimicrobial carbohydrate	137:179	Chitosan oligosaccharide (COS), a time-dependent antimicrobial carbohydrate, is found antifungal active with a short duration of action due to excessive solubility.
34474878	1	22	theme	Chitosan	105:112	arg1	COS					131:133	COS	131:133	COS	131:133	Chitosan oligosaccharide (COS), a time-dependent antimicrobial carbohydrate, is found antifungal active with a short duration of action due to excessive solubility.
34474878	9	23	theme	antifouling	1263:1273	arg1	activity					1275:1282	the antifouling activity	1259:1282	the antifouling activity of the cryogel	1259:1297	With the release of COS, the antifouling activity of the cryogel increased.
34474878	0	24	theme	Macroporous	0:10	arg1	cryogel					35:41	Macroporous zwitterionic composite cryogel	0:41	Macroporous zwitterionic composite cryogel	0:41	Macroporous zwitterionic composite cryogel based on chitosan oligosaccharide for antifungal application.
34474878	8	25	theme	COS/PMarg	1196:1204	arg1	cryogels					1206:1213	the COS/PMarg cryogels	1192:1213	the COS/PMarg cryogels	1192:1213	Swelling capacity, mechanical properties, and COS release behavior of the COS/PMarg cryogels were investigated.
34474878	4	26	theme	magnetic	643:650	arg1	NMR					663:665	NMR	663:665	NMR	663:665	Firstly, Marg was synthesized and characterized by Fourier transform infrared spectroscopy (FT-IR), 1H and 13C nuclear magnetic resonance (NMR), and high-resolution mass spectrometry (HRMS).
34474878	4	26	theme	magnetic	643:650	arg1	resonance					652:660	nuclear magnetic resonance	635:660	nuclear magnetic resonance (NMR)	635:666	Firstly, Marg was synthesized and characterized by Fourier transform infrared spectroscopy (FT-IR), 1H and 13C nuclear magnetic resonance (NMR), and high-resolution mass spectrometry (HRMS).
34474878	5	27	theme	initiation	767:776	arg1	cryopolymerization					778:795	redox initiation cryopolymerization	761:795	redox initiation cryopolymerization	761:795	Then, the COS/PMarg cryogels were prepared by redox initiation cryopolymerization.
34474878	10	28	theme	COS/PMarg	1344:1352	arg1	cryogel					1354:1360	the COS/PMarg cryogel	1340:1360	the COS/PMarg cryogel	1340:1360	Antimicrobial tests indicated the COS/PMarg cryogel could effectively inhibit the proliferation of Candida albicans.
34474878	11	29	theme	macroporous	1452:1462	arg1	cryogel					1497:1503	the macroporous zwitterionic COS/PMarg composite cryogel	1448:1503	the macroporous zwitterionic COS/PMarg composite cryogel	1448:1503	It demonstrated that the macroporous zwitterionic COS/PMarg composite cryogel might be a potential antifungal dressing with sequential "sterilization-release" capacity.
34474878	11	29	theme	macroporous	1452:1462	arg1	dressing					1537:1544	a potential antifungal dressing	1514:1544	a potential antifungal dressing with sequential "sterilization-release" capacity	1514:1593	It demonstrated that the macroporous zwitterionic COS/PMarg composite cryogel might be a potential antifungal dressing with sequential "sterilization-release" capacity.
34474878	3	30	theme	macroporous	365:375	arg1	cryogels					400:407	macroporous zwitterionic composite cryogels	365:407	macroporous zwitterionic composite cryogels composed of COS and poly(N-methacryl arginine) (PMarg)	365:462	In this research, macroporous zwitterionic composite cryogels composed of COS and poly(N-methacryl arginine) (PMarg) were fabricated, serving as long-term antifungal dressings.
34474878	7	31	theme	thermal	1036:1042	arg1	stability					1044:1052	thermal stability	1036:1052	thermal stability of the cryogels	1036:1068	FTIR indicated that hydrogen bonding formed between COS and PMarg, and the interaction elevated thermal stability of the cryogels as evidenced by thermal-gravimetric analysis (TGA).
34474878	4	32	dep	transform	583:591	arg1	infrared					593:600	infrared	593:600	transform infrared spectroscopy (FT-IR), 1H and 13C nuclear magnetic resonance (NMR), and high-resolution mass spectrometry (HRMS)	583:712	Firstly, Marg was synthesized and characterized by Fourier transform infrared spectroscopy (FT-IR), 1H and 13C nuclear magnetic resonance (NMR), and high-resolution mass spectrometry (HRMS).
34474878	1	33	theme	short	216:220	arg1	duration					222:229	a short duration	214:229	a short duration of action due to excessive solubility	214:267	Chitosan oligosaccharide (COS), a time-dependent antimicrobial carbohydrate, is found antifungal active with a short duration of action due to excessive solubility.
34474878	8	34	theme	release	1172:1178	arg1	behavior					1180:1187	COS release behavior	1168:1187	COS release behavior	1168:1187	Swelling capacity, mechanical properties, and COS release behavior of the COS/PMarg cryogels were investigated.
34474878	4	35	dep	Fourier	575:581	arg1	transform					583:591	transform	583:591	transform infrared spectroscopy (FT-IR), 1H and 13C nuclear magnetic resonance (NMR), and high-resolution mass spectrometry (HRMS)	583:712	Firstly, Marg was synthesized and characterized by Fourier transform infrared spectroscopy (FT-IR), 1H and 13C nuclear magnetic resonance (NMR), and high-resolution mass spectrometry (HRMS).
34474878	8	36	theme	cryogels	1206:1213	arg1	behavior					1180:1187	COS release behavior	1168:1187	COS release behavior	1168:1187	Swelling capacity, mechanical properties, and COS release behavior of the COS/PMarg cryogels were investigated.
34474878	8	36	theme	cryogels	1206:1213	arg1	capacity					1131:1138	Swelling capacity	1122:1138	Swelling capacity	1122:1138	Swelling capacity, mechanical properties, and COS release behavior of the COS/PMarg cryogels were investigated.
34474878	8	36	theme	cryogels	1206:1213	arg1	properties					1152:1161	mechanical properties	1141:1161	mechanical properties	1141:1161	Swelling capacity, mechanical properties, and COS release behavior of the COS/PMarg cryogels were investigated.
34474878	8	37	theme	COS	1168:1170	arg1	behavior					1180:1187	COS release behavior	1168:1187	COS release behavior	1168:1187	Swelling capacity, mechanical properties, and COS release behavior of the COS/PMarg cryogels were investigated.
34474878	6	38	from	50.87 μm	930:937	arg1	size					903:906	pore size varying	898:914	pore size varying from 20.86 to 50.87 μm	898:937	The macroporous morphology of the cryogels was confirmed by scanning electron microscope (SEM) with pore size varying from 20.86 to 50.87 μm.
34474878	6	39	theme	cryogels	832:839	arg1	morphology					814:823	The macroporous morphology	798:823	The macroporous morphology of the cryogels	798:839	The macroporous morphology of the cryogels was confirmed by scanning electron microscope (SEM) with pore size varying from 20.86 to 50.87 μm.
34474878	4	40	dep	1H	624:625	arg1	NMR					663:665	NMR	663:665	NMR	663:665	Firstly, Marg was synthesized and characterized by Fourier transform infrared spectroscopy (FT-IR), 1H and 13C nuclear magnetic resonance (NMR), and high-resolution mass spectrometry (HRMS).
34474878	4	40	dep	1H	624:625	arg1	resonance					652:660	nuclear magnetic resonance	635:660	nuclear magnetic resonance (NMR)	635:666	Firstly, Marg was synthesized and characterized by Fourier transform infrared spectroscopy (FT-IR), 1H and 13C nuclear magnetic resonance (NMR), and high-resolution mass spectrometry (HRMS).
34474878	6	41	theme	varying	908:914	arg1	size					903:906	pore size varying	898:914	pore size varying from 20.86 to 50.87 μm	898:937	The macroporous morphology of the cryogels was confirmed by scanning electron microscope (SEM) with pore size varying from 20.86 to 50.87 μm.
34474878	1	42	with	active	202:207	arg1	duration					222:229	a short duration	214:229	a short duration of action due to excessive solubility	214:267	Chitosan oligosaccharide (COS), a time-dependent antimicrobial carbohydrate, is found antifungal active with a short duration of action due to excessive solubility.
34474878	9	43	theme	cryogel	1291:1297	arg1	activity					1275:1282	the antifouling activity	1259:1282	the antifouling activity of the cryogel	1259:1297	With the release of COS, the antifouling activity of the cryogel increased.
34474878	4	44	theme	high-resolution	673:687	arg1	HRMS					708:711	HRMS	708:711	HRMS	708:711	Firstly, Marg was synthesized and characterized by Fourier transform infrared spectroscopy (FT-IR), 1H and 13C nuclear magnetic resonance (NMR), and high-resolution mass spectrometry (HRMS).
34474878	4	44	theme	high-resolution	673:687	arg1	spectrometry					694:705	high-resolution mass spectrometry	673:705	high-resolution mass spectrometry (HRMS)	673:712	Firstly, Marg was synthesized and characterized by Fourier transform infrared spectroscopy (FT-IR), 1H and 13C nuclear magnetic resonance (NMR), and high-resolution mass spectrometry (HRMS).
34474878	0	45	theme	chitosan	52:59	arg1	oligosaccharide					61:75	chitosan oligosaccharide	52:75	chitosan oligosaccharide for antifungal application	52:102	Macroporous zwitterionic composite cryogel based on chitosan oligosaccharide for antifungal application.
34474878	11	46	with	dressing	1537:1544	arg1	capacity					1586:1593	sequential "sterilization-release" capacity	1551:1593	sequential "sterilization-release" capacity	1551:1593	It demonstrated that the macroporous zwitterionic COS/PMarg composite cryogel might be a potential antifungal dressing with sequential "sterilization-release" capacity.
34474878	2	47	theme	COS	334:336	arg1	carrier					338:344	a COS carrier	332:344	a COS carrier	332:344	We attempted to address this issue by employing a hydrogel as a COS carrier.
34474878	2	47	theme	COS	334:336	arg1	hydrogel					320:327	a hydrogel	318:327	a hydrogel	318:327	We attempted to address this issue by employing a hydrogel as a COS carrier.
34474878	11	48	theme	composite	1487:1495	arg1	cryogel					1497:1503	the macroporous zwitterionic COS/PMarg composite cryogel	1448:1503	the macroporous zwitterionic COS/PMarg composite cryogel	1448:1503	It demonstrated that the macroporous zwitterionic COS/PMarg composite cryogel might be a potential antifungal dressing with sequential "sterilization-release" capacity.
34474878	11	48	theme	composite	1487:1495	arg1	dressing					1537:1544	a potential antifungal dressing	1514:1544	a potential antifungal dressing with sequential "sterilization-release" capacity	1514:1593	It demonstrated that the macroporous zwitterionic COS/PMarg composite cryogel might be a potential antifungal dressing with sequential "sterilization-release" capacity.
34474878	6	49	theme	pore	898:901	arg1	size					903:906	pore size varying	898:914	pore size varying from 20.86 to 50.87 μm	898:937	The macroporous morphology of the cryogels was confirmed by scanning electron microscope (SEM) with pore size varying from 20.86 to 50.87 μm.
34474878	1	50	theme	antimicrobial	154:166	arg1	carbohydrate					168:179	a time-dependent antimicrobial carbohydrate	137:179	a time-dependent antimicrobial carbohydrate	137:179	Chitosan oligosaccharide (COS), a time-dependent antimicrobial carbohydrate, is found antifungal active with a short duration of action due to excessive solubility.
34474878	1	50	theme	antimicrobial	154:166	arg1	oligosaccharide					114:128	Chitosan oligosaccharide	105:128	Chitosan oligosaccharide (COS)	105:134	Chitosan oligosaccharide (COS), a time-dependent antimicrobial carbohydrate, is found antifungal active with a short duration of action due to excessive solubility.
34474878	11	51	theme	sterilization-release	1563:1583	arg1	"					1584:1584	sequential "sterilization-release"	1551:1584	sequential "sterilization-release" capacity	1551:1593	It demonstrated that the macroporous zwitterionic COS/PMarg composite cryogel might be a potential antifungal dressing with sequential "sterilization-release" capacity.
34474878	1	52	theme	action	234:239	arg1	duration					222:229	a short duration	214:229	a short duration of action due to excessive solubility	214:267	Chitosan oligosaccharide (COS), a time-dependent antimicrobial carbohydrate, is found antifungal active with a short duration of action due to excessive solubility.
34474878	6	53	theme	macroporous	802:812	arg1	morphology					814:823	The macroporous morphology	798:823	The macroporous morphology of the cryogels	798:839	The macroporous morphology of the cryogels was confirmed by scanning electron microscope (SEM) with pore size varying from 20.86 to 50.87 μm.
34474878	8	54	theme	mechanical	1141:1150	arg1	properties					1152:1161	mechanical properties	1141:1161	mechanical properties	1141:1161	Swelling capacity, mechanical properties, and COS release behavior of the COS/PMarg cryogels were investigated.
34474878	1	55	theme	time-dependent	139:152	arg1	carbohydrate					168:179	a time-dependent antimicrobial carbohydrate	137:179	a time-dependent antimicrobial carbohydrate	137:179	Chitosan oligosaccharide (COS), a time-dependent antimicrobial carbohydrate, is found antifungal active with a short duration of action due to excessive solubility.
34474878	1	55	theme	time-dependent	139:152	arg1	oligosaccharide					114:128	Chitosan oligosaccharide	105:128	Chitosan oligosaccharide (COS)	105:134	Chitosan oligosaccharide (COS), a time-dependent antimicrobial carbohydrate, is found antifungal active with a short duration of action due to excessive solubility.
34474878	11	56	theme	zwitterionic	1464:1475	arg1	cryogel					1497:1503	the macroporous zwitterionic COS/PMarg composite cryogel	1448:1503	the macroporous zwitterionic COS/PMarg composite cryogel	1448:1503	It demonstrated that the macroporous zwitterionic COS/PMarg composite cryogel might be a potential antifungal dressing with sequential "sterilization-release" capacity.
34474878	11	56	theme	zwitterionic	1464:1475	arg1	dressing					1537:1544	a potential antifungal dressing	1514:1544	a potential antifungal dressing with sequential "sterilization-release" capacity	1514:1593	It demonstrated that the macroporous zwitterionic COS/PMarg composite cryogel might be a potential antifungal dressing with sequential "sterilization-release" capacity.
34474878	0	57	theme	antifungal	81:90	arg1	application					92:102	antifungal application	81:102	antifungal application	81:102	Macroporous zwitterionic composite cryogel based on chitosan oligosaccharide for antifungal application.
34474878	6	58	dep	50.87 μm	930:937	arg1	to					927:928	to	927:928	to	927:928	The macroporous morphology of the cryogels was confirmed by scanning electron microscope (SEM) with pore size varying from 20.86 to 50.87 μm.
34474878	7	59	theme	hydrogen	960:967	arg1	bonding					969:975	hydrogen bonding	960:975	hydrogen bonding	960:975	FTIR indicated that hydrogen bonding formed between COS and PMarg, and the interaction elevated thermal stability of the cryogels as evidenced by thermal-gravimetric analysis (TGA).
34474878	3	60	theme	long-term	492:500	arg1	dressings					513:521	long-term antifungal dressings	492:521	long-term antifungal dressings	492:521	In this research, macroporous zwitterionic composite cryogels composed of COS and poly(N-methacryl arginine) (PMarg) were fabricated, serving as long-term antifungal dressings.
34474878	3	61	theme	zwitterionic	377:388	arg1	cryogels					400:407	macroporous zwitterionic composite cryogels	365:407	macroporous zwitterionic composite cryogels composed of COS and poly(N-methacryl arginine) (PMarg)	365:462	In this research, macroporous zwitterionic composite cryogels composed of COS and poly(N-methacryl arginine) (PMarg) were fabricated, serving as long-term antifungal dressings.
34474878	0	62	theme	composite	25:33	arg1	cryogel					35:41	Macroporous zwitterionic composite cryogel	0:41	Macroporous zwitterionic composite cryogel	0:41	Macroporous zwitterionic composite cryogel based on chitosan oligosaccharide for antifungal application.
34474878	3	63	theme	antifungal	502:511	arg1	dressings					513:521	long-term antifungal dressings	492:521	long-term antifungal dressings	492:521	In this research, macroporous zwitterionic composite cryogels composed of COS and poly(N-methacryl arginine) (PMarg) were fabricated, serving as long-term antifungal dressings.
34474878	1	64	theme	excessive	248:256	arg1	solubility					258:267	excessive solubility	248:267	excessive solubility	248:267	Chitosan oligosaccharide (COS), a time-dependent antimicrobial carbohydrate, is found antifungal active with a short duration of action due to excessive solubility.
32478036	2	0	theme	rapid	563:567	arg1	ligation					580:587	rapid amide-bond ligation	563:587	rapid amide-bond ligation	563:587	Herein we developed an alternative glycosylation to construct N-linked glycopeptide via efficient selenoester-assisted aminolysis, which employs the peptidyl ω-asparagine selenoester and unprotected glycosylamine to perform rapid amide-bond ligation.
32478036	1	1	theme	complex	166:172	arg1	glycopeptides					183:195	homogeneous complex N-linked glycopeptides	154:195	homogeneous complex N-linked glycopeptides	154:195	Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field.
32478036	1	2	from	synthesis	294:302	arg1	field					332:336	the related field	320:336	the related field	320:336	Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field.
32478036	3	3	theme	homogeneous	781:791	arg1	glycopeptides					802:814	structure-defined homogeneous N-linked glycopeptides	763:814	structure-defined homogeneous N-linked glycopeptides	763:814	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	3	theme	homogeneous	781:791	arg1	segments					825:832	segments	825:832	segments derived from glycoprotein EPO and IL-5	825:871	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	1	4	gly	glycopeptides	183:195	arg2	glycopeptides					183:195	homogeneous complex N-linked glycopeptides	154:195	homogeneous complex N-linked glycopeptides	154:195	Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field.
32478036	1	5	theme	N-linked	174:181	arg1	glycopeptides					183:195	homogeneous complex N-linked glycopeptides	154:195	homogeneous complex N-linked glycopeptides	154:195	Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field.
32478036	3	6	theme	N-linked	793:800	arg1	glycopeptides					802:814	structure-defined homogeneous N-linked glycopeptides	763:814	structure-defined homogeneous N-linked glycopeptides	763:814	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	6	theme	N-linked	793:800	arg1	segments					825:832	segments	825:832	segments derived from glycoprotein EPO and IL-5	825:871	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	7	theme	structure-defined	763:779	arg1	glycopeptides					802:814	structure-defined homogeneous N-linked glycopeptides	763:814	structure-defined homogeneous N-linked glycopeptides	763:814	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	7	theme	structure-defined	763:779	arg1	segments					825:832	segments	825:832	segments derived from glycoprotein EPO and IL-5	825:871	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	1	8	gly	glycoproteins	201:213	arg1	glycoproteins					201:213	glycoproteins	201:213	glycoproteins	201:213	Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field.
32478036	1	9	theme	Chemical	82:89	arg1	synthesis					91:99	Chemical synthesis	82:99	Chemical synthesis	82:99	Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field.
32478036	1	9	theme	Chemical	82:89	arg1	approach					118:125	an attractive approach	104:125	an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins	104:213	Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field.
32478036	1	10	theme	glycopeptides	183:195	arg1	assembly					142:149	the assembly	138:149	the assembly of homogeneous complex N-linked glycopeptides and glycoproteins	138:213	Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field.
32478036	2	11	link	N-linked	401:408	arg1	glycopeptide					410:421	N-linked glycopeptide	401:421	N-linked glycopeptide	401:421	Herein we developed an alternative glycosylation to construct N-linked glycopeptide via efficient selenoester-assisted aminolysis, which employs the peptidyl ω-asparagine selenoester and unprotected glycosylamine to perform rapid amide-bond ligation.
32478036	2	12	theme	ω-asparagine	497:508	arg1	selenoester					510:520	the peptidyl ω-asparagine selenoester	484:520	the peptidyl ω-asparagine selenoester	484:520	Herein we developed an alternative glycosylation to construct N-linked glycopeptide via efficient selenoester-assisted aminolysis, which employs the peptidyl ω-asparagine selenoester and unprotected glycosylamine to perform rapid amide-bond ligation.
32478036	2	13	theme	efficient	427:435	arg1	aminolysis					458:467	efficient selenoester-assisted aminolysis	427:467	efficient selenoester-assisted aminolysis	427:467	Herein we developed an alternative glycosylation to construct N-linked glycopeptide via efficient selenoester-assisted aminolysis, which employs the peptidyl ω-asparagine selenoester and unprotected glycosylamine to perform rapid amide-bond ligation.
32478036	0	14	theme	ω-Asp	9:13	arg1	Selenoesters					15:26	Peptidyl ω-Asp Selenoesters	0:26	Peptidyl ω-Asp Selenoesters	0:26	Peptidyl ω-Asp Selenoesters Enable Efficient Synthesis of N-Linked Glycopeptides.
32478036	2	15	gly	glycopeptide	410:421	arg2	glycopeptide					410:421	N-linked glycopeptide	401:421	N-linked glycopeptide	401:421	Herein we developed an alternative glycosylation to construct N-linked glycopeptide via efficient selenoester-assisted aminolysis, which employs the peptidyl ω-asparagine selenoester and unprotected glycosylamine to perform rapid amide-bond ligation.
32478036	3	16	theme	carboxylic	653:662	arg1	acids					664:668	free carboxylic acids	648:668	free carboxylic acids	648:668	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	1	17	theme	glycoproteins	201:213	arg1	assembly					142:149	the assembly	138:149	the assembly of homogeneous complex N-linked glycopeptides and glycoproteins	138:213	Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field.
32478036	0	18	theme	Peptidyl	0:7	arg1	Selenoesters					15:26	Peptidyl ω-Asp Selenoesters	0:26	Peptidyl ω-Asp Selenoesters	0:26	Peptidyl ω-Asp Selenoesters Enable Efficient Synthesis of N-Linked Glycopeptides.
32478036	1	19	from	research	308:315	arg1	field					332:336	the related field	320:336	the related field	320:336	Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field.
32478036	3	20	with	compatible	628:637	arg1	groups					683:688	hydroxyl groups	674:688	hydroxyl groups	674:688	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	20	with	compatible	628:637	arg1	carbohydrates					706:718	carbohydrates	706:718	carbohydrates	706:718	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	20	with	compatible	628:637	arg1	peptides					693:700	peptides	693:700	peptides	693:700	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	20	with	compatible	628:637	arg1	acids					664:668	free carboxylic acids	648:668	free carboxylic acids	648:668	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	2	21	theme	peptidyl	488:495	arg1	selenoester					510:520	the peptidyl ω-asparagine selenoester	484:520	the peptidyl ω-asparagine selenoester	484:520	Herein we developed an alternative glycosylation to construct N-linked glycopeptide via efficient selenoester-assisted aminolysis, which employs the peptidyl ω-asparagine selenoester and unprotected glycosylamine to perform rapid amide-bond ligation.
32478036	1	22	theme	attractive	107:116	arg1	synthesis					91:99	Chemical synthesis	82:99	Chemical synthesis	82:99	Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field.
32478036	1	22	theme	attractive	107:116	arg1	approach					118:125	an attractive approach	104:125	an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins	104:213	Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field.
32478036	3	23	link	N-linked	793:800	arg1	glycopeptides					802:814	structure-defined homogeneous N-linked glycopeptides	763:814	structure-defined homogeneous N-linked glycopeptides	763:814	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	23	link	N-linked	793:800	arg1	segments					825:832	segments	825:832	segments derived from glycoprotein EPO and IL-5	825:871	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	1	24	link	N-linked	174:181	arg1	glycopeptides					183:195	homogeneous complex N-linked glycopeptides	154:195	homogeneous complex N-linked glycopeptides	154:195	Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field.
32478036	3	25	theme	free	648:651	arg1	acids					664:668	free carboxylic acids	648:668	free carboxylic acids	648:668	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	2	26	theme	unprotected	526:536	arg1	glycosylamine					538:550	unprotected glycosylamine	526:550	unprotected glycosylamine	526:550	Herein we developed an alternative glycosylation to construct N-linked glycopeptide via efficient selenoester-assisted aminolysis, which employs the peptidyl ω-asparagine selenoester and unprotected glycosylamine to perform rapid amide-bond ligation.
32478036	3	27	theme	glycosylation	595:607	arg1	strategy					609:616	This glycosylation strategy	590:616	This glycosylation strategy	590:616	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	28	theme	glycoprotein	847:858	arg1	EPO					860:862	glycoprotein EPO	847:862	glycoprotein EPO	847:862	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	1	29	theme	limited	224:230	arg1	efficiency					241:250	the limited coupling efficiency	220:250	the limited coupling efficiency between glycans and peptides	220:279	Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field.
32478036	0	30	theme	Efficient	35:43	arg1	Synthesis					45:53	Efficient Synthesis	35:53	Efficient Synthesis of N-Linked Glycopeptides	35:79	Peptidyl ω-Asp Selenoesters Enable Efficient Synthesis of N-Linked Glycopeptides.
32478036	1	31	theme	related	324:330	arg1	field					332:336	the related field	320:336	the related field	320:336	Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field.
32478036	1	32	theme	coupling	232:239	arg1	efficiency					241:250	the limited coupling efficiency	220:250	the limited coupling efficiency between glycans and peptides	220:279	Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field.
32478036	0	33	theme	N-Linked	58:65	arg1	Glycopeptides					67:79	N-Linked Glycopeptides	58:79	N-Linked Glycopeptides	58:79	Peptidyl ω-Asp Selenoesters Enable Efficient Synthesis of N-Linked Glycopeptides.
32478036	3	34	gly	glycopeptides	802:814	arg2	segments					825:832	segments	825:832	segments derived from glycoprotein EPO and IL-5	825:871	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	34	gly	glycopeptides	802:814	arg2	glycopeptides					802:814	structure-defined homogeneous N-linked glycopeptides	763:814	structure-defined homogeneous N-linked glycopeptides	763:814	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	2	35	theme	amide-bond	569:578	arg1	ligation					580:587	rapid amide-bond ligation	563:587	rapid amide-bond ligation	563:587	Herein we developed an alternative glycosylation to construct N-linked glycopeptide via efficient selenoester-assisted aminolysis, which employs the peptidyl ω-asparagine selenoester and unprotected glycosylamine to perform rapid amide-bond ligation.
32478036	3	36	gly	glycoprotein	847:858	arg1	glycoprotein					847:858	glycoprotein EPO	847:862	glycoprotein EPO	847:862	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	2	37	theme	alternative	362:372	arg1	glycosylation					374:386	an alternative glycosylation	359:386	an alternative glycosylation to construct N-linked glycopeptide via efficient selenoester-assisted aminolysis, which employs the peptidyl ω-asparagine selenoester and unprotected glycosylamine to perform rapid amide-bond ligation	359:587	Herein we developed an alternative glycosylation to construct N-linked glycopeptide via efficient selenoester-assisted aminolysis, which employs the peptidyl ω-asparagine selenoester and unprotected glycosylamine to perform rapid amide-bond ligation.
32478036	2	38	theme	selenoester-assisted	437:456	arg1	aminolysis					458:467	efficient selenoester-assisted aminolysis	427:467	efficient selenoester-assisted aminolysis	427:467	Herein we developed an alternative glycosylation to construct N-linked glycopeptide via efficient selenoester-assisted aminolysis, which employs the peptidyl ω-asparagine selenoester and unprotected glycosylamine to perform rapid amide-bond ligation.
32478036	3	39	theme	hydroxyl	674:681	arg1	groups					683:688	hydroxyl groups	674:688	hydroxyl groups	674:688	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	39	theme	hydroxyl	674:681	arg1	carbohydrates					706:718	carbohydrates	706:718	carbohydrates	706:718	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	39	theme	hydroxyl	674:681	arg1	peptides					693:700	peptides	693:700	peptides	693:700	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	1	40	dep	synthesis	294:302	arg1	the					290:292	the	290:292	the	290:292	Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field.
32478036	3	41	theme	carbohydrates	706:718	arg1	groups					683:688	hydroxyl groups	674:688	hydroxyl groups	674:688	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	41	theme	carbohydrates	706:718	arg1	carbohydrates					706:718	carbohydrates	706:718	carbohydrates	706:718	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	41	theme	carbohydrates	706:718	arg1	peptides					693:700	peptides	693:700	peptides	693:700	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	41	theme	carbohydrates	706:718	arg1	acids					664:668	free carboxylic acids	648:668	free carboxylic acids	648:668	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	1	42	theme	homogeneous	154:164	arg1	glycopeptides					183:195	homogeneous complex N-linked glycopeptides	154:195	homogeneous complex N-linked glycopeptides	154:195	Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field.
32478036	3	43	attach	derived	834:840	arg1	EPO					860:862	glycoprotein EPO	847:862	glycoprotein EPO	847:862	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	43	attach	derived	834:840	arg2	segments					825:832	segments	825:832	segments derived from glycoprotein EPO and IL-5	825:871	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	43	attach	derived	834:840	arg1	IL-5					868:871	IL-5	868:871	IL-5	868:871	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	44	theme	peptides	693:700	arg1	groups					683:688	hydroxyl groups	674:688	hydroxyl groups	674:688	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	44	theme	peptides	693:700	arg1	carbohydrates					706:718	carbohydrates	706:718	carbohydrates	706:718	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	44	theme	peptides	693:700	arg1	peptides					693:700	peptides	693:700	peptides	693:700	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	3	44	theme	peptides	693:700	arg1	acids					664:668	free carboxylic acids	648:668	free carboxylic acids	648:668	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	2	45	theme	N-linked	401:408	arg1	glycopeptide					410:421	N-linked glycopeptide	401:421	N-linked glycopeptide	401:421	Herein we developed an alternative glycosylation to construct N-linked glycopeptide via efficient selenoester-assisted aminolysis, which employs the peptidyl ω-asparagine selenoester and unprotected glycosylamine to perform rapid amide-bond ligation.
32478036	3	46	dep	acids	664:668	arg1	the					644:646	the	644:646	the	644:646	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	0	47	theme	Glycopeptides	67:79	arg1	Synthesis					45:53	Efficient Synthesis	35:53	Efficient Synthesis of N-Linked Glycopeptides	35:79	Peptidyl ω-Asp Selenoesters Enable Efficient Synthesis of N-Linked Glycopeptides.
32478036	3	48	theme	glycopeptides	802:814	arg1	assembly					751:758	the assembly	747:758	the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5	747:871	This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.
32478036	1	49	dep	approach	118:125	arg1	allows					127:132	allows	127:132	allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins	127:213	Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field.
34739010	0	0	theme	type	89:92	arg1	mellitus					105:112	type 2 diabetes mellitus	89:112	type 2 diabetes mellitus in rats	89:120	The positive effects and underlying mechanisms of Undaria pinnatifida polysaccharides on type 2 diabetes mellitus in rats.
34739010	0	1	from	effects	13:19	arg1	mellitus					105:112	type 2 diabetes mellitus	89:112	type 2 diabetes mellitus in rats	89:120	The positive effects and underlying mechanisms of Undaria pinnatifida polysaccharides on type 2 diabetes mellitus in rats.
34739010	4	2	theme	rRNA	682:685	arg1	analysis					687:694	The 16S rRNA analysis	674:694	The 16S rRNA analysis	674:694	The 16S rRNA analysis demonstrated that UPP intervention could markedly change the intestinal microflora composition, causing increases in Alistipes, Bacteroides, Christensenellaceae_R-7_group, Desulfovibrio, Muribaculaceae_norank, Ruminococcaceae_UCG-013, and Ruminococcaceae_UCG-014, and a decrease in Escherichia-Shigella.
34739010	1	3	theme	current	138:144	arg1	work					146:149	the current work	134:149	the current work	134:149	The aim of the current work was to investigate the anti-diabetic effects and underlying mechanisms of Undaria pinnatifida polysaccharides (UPP) based on a type 2 diabetes (T2DM) rat model.
34739010	1	4	theme	polysaccharides	245:259	arg1	effects					188:194	anti-diabetic effects	174:194	anti-diabetic effects	174:194	The aim of the current work was to investigate the anti-diabetic effects and underlying mechanisms of Undaria pinnatifida polysaccharides (UPP) based on a type 2 diabetes (T2DM) rat model.
34739010	1	4	theme	polysaccharides	245:259	arg1	mechanisms					211:220	underlying mechanisms	200:220	underlying mechanisms	200:220	The aim of the current work was to investigate the anti-diabetic effects and underlying mechanisms of Undaria pinnatifida polysaccharides (UPP) based on a type 2 diabetes (T2DM) rat model.
34739010	1	5	theme	rat	301:303	arg1	model					305:309	a type 2 diabetes (T2DM) rat model	276:309	a type 2 diabetes (T2DM) rat model	276:309	The aim of the current work was to investigate the anti-diabetic effects and underlying mechanisms of Undaria pinnatifida polysaccharides (UPP) based on a type 2 diabetes (T2DM) rat model.
34739010	5	6	theme	Egr-1	1160:1164	arg1	genes					1166:1170	Egr-1 genes	1160:1170	Egr-1 genes	1160:1170	Furthermore, RT-qPCR analysis results clarified that UPP administration distinctly activated the IRS/PI3K/AKT signaling pathway, restrained PEPCK, G-6-Pase and Egr-1 genes, and affected the relative expression of HMGCR and LDLR genes.
34739010	1	7	theme	work	146:149	arg1	aim					127:129	The aim	123:129	The aim of the current work	123:149	The aim of the current work was to investigate the anti-diabetic effects and underlying mechanisms of Undaria pinnatifida polysaccharides (UPP) based on a type 2 diabetes (T2DM) rat model.
34739010	0	8	theme	diabetes	96:103	arg1	mellitus					105:112	type 2 diabetes mellitus	89:112	type 2 diabetes mellitus in rats	89:120	The positive effects and underlying mechanisms of Undaria pinnatifida polysaccharides on type 2 diabetes mellitus in rats.
34739010	5	9	theme	HMGCR	1213:1217	arg1	expression					1199:1208	the relative expression	1186:1208	the relative expression of HMGCR and LDLR genes	1186:1232	Furthermore, RT-qPCR analysis results clarified that UPP administration distinctly activated the IRS/PI3K/AKT signaling pathway, restrained PEPCK, G-6-Pase and Egr-1 genes, and affected the relative expression of HMGCR and LDLR genes.
34739010	5	10	theme	signaling	1110:1118	arg1	pathway					1120:1126	the IRS/PI3K/AKT signaling pathway	1093:1126	the IRS/PI3K/AKT signaling pathway	1093:1126	Furthermore, RT-qPCR analysis results clarified that UPP administration distinctly activated the IRS/PI3K/AKT signaling pathway, restrained PEPCK, G-6-Pase and Egr-1 genes, and affected the relative expression of HMGCR and LDLR genes.
34739010	3	11	theme	fasting	528:534	arg1	level					550:554	fasting blood glucose level	528:554	fasting blood glucose level	528:554	Analysis of diabetic symptoms and biochemical profiles showed that UPP intervention markedly decreased fasting blood glucose level, mitigated insulin resistance, improved glucose tolerance, dyslipidemia and liver and kidney damage in diabetic rats.
34739010	5	12	theme	RT-qPCR	1013:1019	arg1	analysis					1021:1028	RT-qPCR analysis	1013:1028	RT-qPCR analysis	1013:1028	Furthermore, RT-qPCR analysis results clarified that UPP administration distinctly activated the IRS/PI3K/AKT signaling pathway, restrained PEPCK, G-6-Pase and Egr-1 genes, and affected the relative expression of HMGCR and LDLR genes.
34739010	3	13	from	liver	632:636	arg1	rats					668:671	diabetic rats	659:671	diabetic rats	659:671	Analysis of diabetic symptoms and biochemical profiles showed that UPP intervention markedly decreased fasting blood glucose level, mitigated insulin resistance, improved glucose tolerance, dyslipidemia and liver and kidney damage in diabetic rats.
34739010	3	14	theme	blood	536:540	arg1	level					550:554	fasting blood glucose level	528:554	fasting blood glucose level	528:554	Analysis of diabetic symptoms and biochemical profiles showed that UPP intervention markedly decreased fasting blood glucose level, mitigated insulin resistance, improved glucose tolerance, dyslipidemia and liver and kidney damage in diabetic rats.
34739010	6	15	theme	T2DM	1328:1331	arg1	management					1314:1323	the management	1310:1323	the management of T2DM	1310:1331	This study demonstrates that UPP could be applied as an adjuvant agent for the management of T2DM.
34739010	2	16	theme	starch	316:321	arg1	test					331:334	The starch loading test	312:334	The starch loading test	312:334	The starch loading test showed that UPP administration could reduce blood glucose fluctuations caused by eating.
34739010	3	17	theme	diabetic	437:444	arg1	symptoms					446:453	diabetic symptoms	437:453	diabetic symptoms	437:453	Analysis of diabetic symptoms and biochemical profiles showed that UPP intervention markedly decreased fasting blood glucose level, mitigated insulin resistance, improved glucose tolerance, dyslipidemia and liver and kidney damage in diabetic rats.
34739010	3	18	from	tolerance	604:612	arg1	rats					668:671	diabetic rats	659:671	diabetic rats	659:671	Analysis of diabetic symptoms and biochemical profiles showed that UPP intervention markedly decreased fasting blood glucose level, mitigated insulin resistance, improved glucose tolerance, dyslipidemia and liver and kidney damage in diabetic rats.
34739010	0	19	from	mechanisms	36:45	arg1	mellitus					105:112	type 2 diabetes mellitus	89:112	type 2 diabetes mellitus in rats	89:120	The positive effects and underlying mechanisms of Undaria pinnatifida polysaccharides on type 2 diabetes mellitus in rats.
34739010	4	20	theme	microflora	768:777	arg1	composition					779:789	the intestinal microflora composition	753:789	the intestinal microflora composition	753:789	The 16S rRNA analysis demonstrated that UPP intervention could markedly change the intestinal microflora composition, causing increases in Alistipes, Bacteroides, Christensenellaceae_R-7_group, Desulfovibrio, Muribaculaceae_norank, Ruminococcaceae_UCG-013, and Ruminococcaceae_UCG-014, and a decrease in Escherichia-Shigella.
34739010	2	21	theme	UPP	348:350	arg1	administration					352:365	UPP administration	348:365	UPP administration	348:365	The starch loading test showed that UPP administration could reduce blood glucose fluctuations caused by eating.
34739010	0	22	theme	positive	4:11	arg1	effects					13:19	positive effects	4:19	positive effects	4:19	The positive effects and underlying mechanisms of Undaria pinnatifida polysaccharides on type 2 diabetes mellitus in rats.
34739010	3	23	from	damage	649:654	arg1	rats					668:671	diabetic rats	659:671	diabetic rats	659:671	Analysis of diabetic symptoms and biochemical profiles showed that UPP intervention markedly decreased fasting blood glucose level, mitigated insulin resistance, improved glucose tolerance, dyslipidemia and liver and kidney damage in diabetic rats.
34739010	6	24	theme	adjuvant	1291:1298	arg1	UPP					1264:1266	UPP	1264:1266	UPP	1264:1266	This study demonstrates that UPP could be applied as an adjuvant agent for the management of T2DM.
34739010	6	24	theme	adjuvant	1291:1298	arg1	agent					1300:1304	an adjuvant agent	1288:1304	an adjuvant agent for the management of T2DM	1288:1331	This study demonstrates that UPP could be applied as an adjuvant agent for the management of T2DM.
34739010	3	25	from	dyslipidemia	615:626	arg1	rats					668:671	diabetic rats	659:671	diabetic rats	659:671	Analysis of diabetic symptoms and biochemical profiles showed that UPP intervention markedly decreased fasting blood glucose level, mitigated insulin resistance, improved glucose tolerance, dyslipidemia and liver and kidney damage in diabetic rats.
34739010	4	26	theme	intestinal	757:766	arg1	composition					779:789	the intestinal microflora composition	753:789	the intestinal microflora composition	753:789	The 16S rRNA analysis demonstrated that UPP intervention could markedly change the intestinal microflora composition, causing increases in Alistipes, Bacteroides, Christensenellaceae_R-7_group, Desulfovibrio, Muribaculaceae_norank, Ruminococcaceae_UCG-013, and Ruminococcaceae_UCG-014, and a decrease in Escherichia-Shigella.
34739010	3	27	theme	glucose	542:548	arg1	level					550:554	fasting blood glucose level	528:554	fasting blood glucose level	528:554	Analysis of diabetic symptoms and biochemical profiles showed that UPP intervention markedly decreased fasting blood glucose level, mitigated insulin resistance, improved glucose tolerance, dyslipidemia and liver and kidney damage in diabetic rats.
34739010	3	28	from	resistance	575:584	arg1	rats					668:671	diabetic rats	659:671	diabetic rats	659:671	Analysis of diabetic symptoms and biochemical profiles showed that UPP intervention markedly decreased fasting blood glucose level, mitigated insulin resistance, improved glucose tolerance, dyslipidemia and liver and kidney damage in diabetic rats.
34739010	1	29	theme	anti-diabetic	174:186	arg1	effects					188:194	anti-diabetic effects	174:194	anti-diabetic effects	174:194	The aim of the current work was to investigate the anti-diabetic effects and underlying mechanisms of Undaria pinnatifida polysaccharides (UPP) based on a type 2 diabetes (T2DM) rat model.
34739010	4	30	from	increases	800:808	arg1	Christensenellaceae_R-7_group					837:865	Christensenellaceae_R-7_group	837:865	Christensenellaceae_R-7_group	837:865	The 16S rRNA analysis demonstrated that UPP intervention could markedly change the intestinal microflora composition, causing increases in Alistipes, Bacteroides, Christensenellaceae_R-7_group, Desulfovibrio, Muribaculaceae_norank, Ruminococcaceae_UCG-013, and Ruminococcaceae_UCG-014, and a decrease in Escherichia-Shigella.
34739010	4	30	from	increases	800:808	arg1	Escherichia-Shigella					978:997	Escherichia-Shigella	978:997	Escherichia-Shigella	978:997	The 16S rRNA analysis demonstrated that UPP intervention could markedly change the intestinal microflora composition, causing increases in Alistipes, Bacteroides, Christensenellaceae_R-7_group, Desulfovibrio, Muribaculaceae_norank, Ruminococcaceae_UCG-013, and Ruminococcaceae_UCG-014, and a decrease in Escherichia-Shigella.
34739010	4	30	from	increases	800:808	arg1	Desulfovibrio					868:880	Desulfovibrio	868:880	Desulfovibrio	868:880	The 16S rRNA analysis demonstrated that UPP intervention could markedly change the intestinal microflora composition, causing increases in Alistipes, Bacteroides, Christensenellaceae_R-7_group, Desulfovibrio, Muribaculaceae_norank, Ruminococcaceae_UCG-013, and Ruminococcaceae_UCG-014, and a decrease in Escherichia-Shigella.
34739010	4	30	from	increases	800:808	arg1	Ruminococcaceae_UCG-014					935:957	Ruminococcaceae_UCG-014	935:957	Ruminococcaceae_UCG-014	935:957	The 16S rRNA analysis demonstrated that UPP intervention could markedly change the intestinal microflora composition, causing increases in Alistipes, Bacteroides, Christensenellaceae_R-7_group, Desulfovibrio, Muribaculaceae_norank, Ruminococcaceae_UCG-013, and Ruminococcaceae_UCG-014, and a decrease in Escherichia-Shigella.
34739010	4	30	from	increases	800:808	arg1	Bacteroides					824:834	Bacteroides	824:834	Bacteroides	824:834	The 16S rRNA analysis demonstrated that UPP intervention could markedly change the intestinal microflora composition, causing increases in Alistipes, Bacteroides, Christensenellaceae_R-7_group, Desulfovibrio, Muribaculaceae_norank, Ruminococcaceae_UCG-013, and Ruminococcaceae_UCG-014, and a decrease in Escherichia-Shigella.
34739010	4	30	from	increases	800:808	arg1	Muribaculaceae_norank					883:903	Muribaculaceae_norank	883:903	Muribaculaceae_norank	883:903	The 16S rRNA analysis demonstrated that UPP intervention could markedly change the intestinal microflora composition, causing increases in Alistipes, Bacteroides, Christensenellaceae_R-7_group, Desulfovibrio, Muribaculaceae_norank, Ruminococcaceae_UCG-013, and Ruminococcaceae_UCG-014, and a decrease in Escherichia-Shigella.
34739010	4	30	from	increases	800:808	arg1	Ruminococcaceae_UCG-013					906:928	Ruminococcaceae_UCG-013	906:928	Ruminococcaceae_UCG-013	906:928	The 16S rRNA analysis demonstrated that UPP intervention could markedly change the intestinal microflora composition, causing increases in Alistipes, Bacteroides, Christensenellaceae_R-7_group, Desulfovibrio, Muribaculaceae_norank, Ruminococcaceae_UCG-013, and Ruminococcaceae_UCG-014, and a decrease in Escherichia-Shigella.
34739010	4	31	theme	16S	678:680	arg1	rRNA					682:685	The 16S rRNA	674:685	The 16S rRNA analysis	674:694	The 16S rRNA analysis demonstrated that UPP intervention could markedly change the intestinal microflora composition, causing increases in Alistipes, Bacteroides, Christensenellaceae_R-7_group, Desulfovibrio, Muribaculaceae_norank, Ruminococcaceae_UCG-013, and Ruminococcaceae_UCG-014, and a decrease in Escherichia-Shigella.
34739010	2	32	theme	blood	380:384	arg1	fluctuations					394:405	blood glucose fluctuations	380:405	blood glucose fluctuations caused by eating	380:422	The starch loading test showed that UPP administration could reduce blood glucose fluctuations caused by eating.
34739010	1	33	theme	type	278:281	arg1	diabetes					285:292	type 2 diabetes	278:292	a type 2 diabetes (T2DM) rat model	276:309	The aim of the current work was to investigate the anti-diabetic effects and underlying mechanisms of Undaria pinnatifida polysaccharides (UPP) based on a type 2 diabetes (T2DM) rat model.
34739010	1	33	theme	type	278:281	arg1	T2DM					295:298	T2DM	295:298	T2DM	295:298	The aim of the current work was to investigate the anti-diabetic effects and underlying mechanisms of Undaria pinnatifida polysaccharides (UPP) based on a type 2 diabetes (T2DM) rat model.
34739010	3	34	theme	kidney	642:647	arg1	damage					649:654	kidney damage	642:654	kidney damage	642:654	Analysis of diabetic symptoms and biochemical profiles showed that UPP intervention markedly decreased fasting blood glucose level, mitigated insulin resistance, improved glucose tolerance, dyslipidemia and liver and kidney damage in diabetic rats.
34739010	0	35	theme	underlying	25:34	arg1	mechanisms					36:45	underlying mechanisms	25:45	underlying mechanisms	25:45	The positive effects and underlying mechanisms of Undaria pinnatifida polysaccharides on type 2 diabetes mellitus in rats.
34739010	4	36	from	decrease	966:973	arg1	Christensenellaceae_R-7_group					837:865	Christensenellaceae_R-7_group	837:865	Christensenellaceae_R-7_group	837:865	The 16S rRNA analysis demonstrated that UPP intervention could markedly change the intestinal microflora composition, causing increases in Alistipes, Bacteroides, Christensenellaceae_R-7_group, Desulfovibrio, Muribaculaceae_norank, Ruminococcaceae_UCG-013, and Ruminococcaceae_UCG-014, and a decrease in Escherichia-Shigella.
34739010	4	36	from	decrease	966:973	arg1	Escherichia-Shigella					978:997	Escherichia-Shigella	978:997	Escherichia-Shigella	978:997	The 16S rRNA analysis demonstrated that UPP intervention could markedly change the intestinal microflora composition, causing increases in Alistipes, Bacteroides, Christensenellaceae_R-7_group, Desulfovibrio, Muribaculaceae_norank, Ruminococcaceae_UCG-013, and Ruminococcaceae_UCG-014, and a decrease in Escherichia-Shigella.
34739010	4	36	from	decrease	966:973	arg1	Desulfovibrio					868:880	Desulfovibrio	868:880	Desulfovibrio	868:880	The 16S rRNA analysis demonstrated that UPP intervention could markedly change the intestinal microflora composition, causing increases in Alistipes, Bacteroides, Christensenellaceae_R-7_group, Desulfovibrio, Muribaculaceae_norank, Ruminococcaceae_UCG-013, and Ruminococcaceae_UCG-014, and a decrease in Escherichia-Shigella.
34739010	4	36	from	decrease	966:973	arg1	Ruminococcaceae_UCG-014					935:957	Ruminococcaceae_UCG-014	935:957	Ruminococcaceae_UCG-014	935:957	The 16S rRNA analysis demonstrated that UPP intervention could markedly change the intestinal microflora composition, causing increases in Alistipes, Bacteroides, Christensenellaceae_R-7_group, Desulfovibrio, Muribaculaceae_norank, Ruminococcaceae_UCG-013, and Ruminococcaceae_UCG-014, and a decrease in Escherichia-Shigella.
34739010	4	36	from	decrease	966:973	arg1	Bacteroides					824:834	Bacteroides	824:834	Bacteroides	824:834	The 16S rRNA analysis demonstrated that UPP intervention could markedly change the intestinal microflora composition, causing increases in Alistipes, Bacteroides, Christensenellaceae_R-7_group, Desulfovibrio, Muribaculaceae_norank, Ruminococcaceae_UCG-013, and Ruminococcaceae_UCG-014, and a decrease in Escherichia-Shigella.
34739010	4	36	from	decrease	966:973	arg1	Muribaculaceae_norank					883:903	Muribaculaceae_norank	883:903	Muribaculaceae_norank	883:903	The 16S rRNA analysis demonstrated that UPP intervention could markedly change the intestinal microflora composition, causing increases in Alistipes, Bacteroides, Christensenellaceae_R-7_group, Desulfovibrio, Muribaculaceae_norank, Ruminococcaceae_UCG-013, and Ruminococcaceae_UCG-014, and a decrease in Escherichia-Shigella.
34739010	4	36	from	decrease	966:973	arg1	Ruminococcaceae_UCG-013					906:928	Ruminococcaceae_UCG-013	906:928	Ruminococcaceae_UCG-013	906:928	The 16S rRNA analysis demonstrated that UPP intervention could markedly change the intestinal microflora composition, causing increases in Alistipes, Bacteroides, Christensenellaceae_R-7_group, Desulfovibrio, Muribaculaceae_norank, Ruminococcaceae_UCG-013, and Ruminococcaceae_UCG-014, and a decrease in Escherichia-Shigella.
34739010	3	37	theme	improved	587:594	arg1	tolerance					604:612	improved glucose tolerance	587:612	improved glucose tolerance	587:612	Analysis of diabetic symptoms and biochemical profiles showed that UPP intervention markedly decreased fasting blood glucose level, mitigated insulin resistance, improved glucose tolerance, dyslipidemia and liver and kidney damage in diabetic rats.
34739010	1	38	theme	underlying	200:209	arg1	mechanisms					211:220	underlying mechanisms	200:220	underlying mechanisms	200:220	The aim of the current work was to investigate the anti-diabetic effects and underlying mechanisms of Undaria pinnatifida polysaccharides (UPP) based on a type 2 diabetes (T2DM) rat model.
34739010	0	39	theme	Undaria	50:56	arg1	polysaccharides					70:84	Undaria pinnatifida polysaccharides	50:84	Undaria pinnatifida polysaccharides	50:84	The positive effects and underlying mechanisms of Undaria pinnatifida polysaccharides on type 2 diabetes mellitus in rats.
34739010	1	40	theme	diabetes	285:292	arg1	model					305:309	a type 2 diabetes (T2DM) rat model	276:309	a type 2 diabetes (T2DM) rat model	276:309	The aim of the current work was to investigate the anti-diabetic effects and underlying mechanisms of Undaria pinnatifida polysaccharides (UPP) based on a type 2 diabetes (T2DM) rat model.
34739010	3	41	theme	insulin	567:573	arg1	resistance					575:584	insulin resistance	567:584	insulin resistance	567:584	Analysis of diabetic symptoms and biochemical profiles showed that UPP intervention markedly decreased fasting blood glucose level, mitigated insulin resistance, improved glucose tolerance, dyslipidemia and liver and kidney damage in diabetic rats.
34739010	3	42	theme	biochemical	459:469	arg1	profiles					471:478	biochemical profiles	459:478	biochemical profiles	459:478	Analysis of diabetic symptoms and biochemical profiles showed that UPP intervention markedly decreased fasting blood glucose level, mitigated insulin resistance, improved glucose tolerance, dyslipidemia and liver and kidney damage in diabetic rats.
34739010	5	43	theme	genes	1228:1232	arg1	expression					1199:1208	the relative expression	1186:1208	the relative expression of HMGCR and LDLR genes	1186:1232	Furthermore, RT-qPCR analysis results clarified that UPP administration distinctly activated the IRS/PI3K/AKT signaling pathway, restrained PEPCK, G-6-Pase and Egr-1 genes, and affected the relative expression of HMGCR and LDLR genes.
34739010	5	44	theme	relative	1190:1197	arg1	expression					1199:1208	the relative expression	1186:1208	the relative expression of HMGCR and LDLR genes	1186:1232	Furthermore, RT-qPCR analysis results clarified that UPP administration distinctly activated the IRS/PI3K/AKT signaling pathway, restrained PEPCK, G-6-Pase and Egr-1 genes, and affected the relative expression of HMGCR and LDLR genes.
34739010	3	45	theme	diabetic	659:666	arg1	rats					668:671	diabetic rats	659:671	diabetic rats	659:671	Analysis of diabetic symptoms and biochemical profiles showed that UPP intervention markedly decreased fasting blood glucose level, mitigated insulin resistance, improved glucose tolerance, dyslipidemia and liver and kidney damage in diabetic rats.
34739010	3	46	theme	profiles	471:478	arg1	Analysis					425:432	Analysis	425:432	Analysis of diabetic symptoms and biochemical profiles	425:478	Analysis of diabetic symptoms and biochemical profiles showed that UPP intervention markedly decreased fasting blood glucose level, mitigated insulin resistance, improved glucose tolerance, dyslipidemia and liver and kidney damage in diabetic rats.
34739010	0	47	from	mellitus	105:112	arg1	rats					117:120	rats	117:120	rats	117:120	The positive effects and underlying mechanisms of Undaria pinnatifida polysaccharides on type 2 diabetes mellitus in rats.
34739010	5	48	theme	UPP	1053:1055	arg1	administration					1057:1070	UPP administration	1053:1070	UPP administration	1053:1070	Furthermore, RT-qPCR analysis results clarified that UPP administration distinctly activated the IRS/PI3K/AKT signaling pathway, restrained PEPCK, G-6-Pase and Egr-1 genes, and affected the relative expression of HMGCR and LDLR genes.
34739010	5	49	theme	IRS/PI3K/AKT	1097:1108	arg1	pathway					1120:1126	the IRS/PI3K/AKT signaling pathway	1093:1126	the IRS/PI3K/AKT signaling pathway	1093:1126	Furthermore, RT-qPCR analysis results clarified that UPP administration distinctly activated the IRS/PI3K/AKT signaling pathway, restrained PEPCK, G-6-Pase and Egr-1 genes, and affected the relative expression of HMGCR and LDLR genes.
34739010	3	50	theme	symptoms	446:453	arg1	Analysis					425:432	Analysis	425:432	Analysis of diabetic symptoms and biochemical profiles	425:478	Analysis of diabetic symptoms and biochemical profiles showed that UPP intervention markedly decreased fasting blood glucose level, mitigated insulin resistance, improved glucose tolerance, dyslipidemia and liver and kidney damage in diabetic rats.
34739010	0	51	dep	effects	13:19	arg1	The					0:2	The	0:2	The	0:2	The positive effects and underlying mechanisms of Undaria pinnatifida polysaccharides on type 2 diabetes mellitus in rats.
34739010	5	52	dep	results	1030:1036	arg1	clarified					1038:1046	clarified	1038:1046	results clarified that UPP administration distinctly activated the IRS/PI3K/AKT signaling pathway, restrained PEPCK, G-6-Pase and Egr-1 genes, and affected the relative expression of HMGCR and LDLR genes	1030:1232	Furthermore, RT-qPCR analysis results clarified that UPP administration distinctly activated the IRS/PI3K/AKT signaling pathway, restrained PEPCK, G-6-Pase and Egr-1 genes, and affected the relative expression of HMGCR and LDLR genes.
34739010	4	53	theme	UPP	714:716	arg1	intervention					718:729	UPP intervention	714:729	UPP intervention	714:729	The 16S rRNA analysis demonstrated that UPP intervention could markedly change the intestinal microflora composition, causing increases in Alistipes, Bacteroides, Christensenellaceae_R-7_group, Desulfovibrio, Muribaculaceae_norank, Ruminococcaceae_UCG-013, and Ruminococcaceae_UCG-014, and a decrease in Escherichia-Shigella.
34739010	0	54	theme	polysaccharides	70:84	arg1	effects					13:19	positive effects	4:19	positive effects	4:19	The positive effects and underlying mechanisms of Undaria pinnatifida polysaccharides on type 2 diabetes mellitus in rats.
34739010	0	54	theme	polysaccharides	70:84	arg1	mechanisms					36:45	underlying mechanisms	25:45	underlying mechanisms	25:45	The positive effects and underlying mechanisms of Undaria pinnatifida polysaccharides on type 2 diabetes mellitus in rats.
34739010	3	55	theme	glucose	596:602	arg1	tolerance					604:612	improved glucose tolerance	587:612	improved glucose tolerance	587:612	Analysis of diabetic symptoms and biochemical profiles showed that UPP intervention markedly decreased fasting blood glucose level, mitigated insulin resistance, improved glucose tolerance, dyslipidemia and liver and kidney damage in diabetic rats.
34739010	2	56	theme	glucose	386:392	arg1	fluctuations					394:405	blood glucose fluctuations	380:405	blood glucose fluctuations caused by eating	380:422	The starch loading test showed that UPP administration could reduce blood glucose fluctuations caused by eating.
34739010	3	57	theme	UPP	492:494	arg1	intervention					496:507	UPP intervention	492:507	UPP intervention	492:507	Analysis of diabetic symptoms and biochemical profiles showed that UPP intervention markedly decreased fasting blood glucose level, mitigated insulin resistance, improved glucose tolerance, dyslipidemia and liver and kidney damage in diabetic rats.
34739010	1	58	theme	Undaria	225:231	arg1	UPP					262:264	UPP	262:264	UPP	262:264	The aim of the current work was to investigate the anti-diabetic effects and underlying mechanisms of Undaria pinnatifida polysaccharides (UPP) based on a type 2 diabetes (T2DM) rat model.
34739010	1	58	theme	Undaria	225:231	arg1	polysaccharides					245:259	Undaria pinnatifida polysaccharides	225:259	Undaria pinnatifida polysaccharides (UPP)	225:265	The aim of the current work was to investigate the anti-diabetic effects and underlying mechanisms of Undaria pinnatifida polysaccharides (UPP) based on a type 2 diabetes (T2DM) rat model.
34739010	0	59	theme	pinnatifida	58:68	arg1	polysaccharides					70:84	Undaria pinnatifida polysaccharides	50:84	Undaria pinnatifida polysaccharides	50:84	The positive effects and underlying mechanisms of Undaria pinnatifida polysaccharides on type 2 diabetes mellitus in rats.
34739010	5	60	theme	LDLR	1223:1226	arg1	genes					1228:1232	LDLR genes	1223:1232	LDLR genes	1223:1232	Furthermore, RT-qPCR analysis results clarified that UPP administration distinctly activated the IRS/PI3K/AKT signaling pathway, restrained PEPCK, G-6-Pase and Egr-1 genes, and affected the relative expression of HMGCR and LDLR genes.
34739010	1	61	dep	effects	188:194	arg1	the					170:172	the	170:172	the	170:172	The aim of the current work was to investigate the anti-diabetic effects and underlying mechanisms of Undaria pinnatifida polysaccharides (UPP) based on a type 2 diabetes (T2DM) rat model.
34739010	2	62	theme	loading	323:329	arg1	test					331:334	The starch loading test	312:334	The starch loading test	312:334	The starch loading test showed that UPP administration could reduce blood glucose fluctuations caused by eating.
34739010	1	63	theme	pinnatifida	233:243	arg1	UPP					262:264	UPP	262:264	UPP	262:264	The aim of the current work was to investigate the anti-diabetic effects and underlying mechanisms of Undaria pinnatifida polysaccharides (UPP) based on a type 2 diabetes (T2DM) rat model.
34739010	1	63	theme	pinnatifida	233:243	arg1	polysaccharides					245:259	Undaria pinnatifida polysaccharides	225:259	Undaria pinnatifida polysaccharides (UPP)	225:265	The aim of the current work was to investigate the anti-diabetic effects and underlying mechanisms of Undaria pinnatifida polysaccharides (UPP) based on a type 2 diabetes (T2DM) rat model.
34965089	2	0	theme	%	614:614	arg1	acid					653:656	55 mol % 2-acrylamido-2-methylpropanesulfonic acid	607:656	55 mol % 2-acrylamido-2-methylpropanesulfonic acid	607:656	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	3	1	theme	functional	930:939	arg1	groups					941:946	environmental functional groups	916:946	environmental functional groups	916:946	The surface morphology obtained by calcite growth under surface or environmental functional groups' influence and the hydrophilic/hydrophobic character of the composite materials were followed and compared to that of the starting material.
34965089	5	2	theme	modified	1253:1260	arg1	surfaces					1262:1269	selected modified surfaces	1244:1269	selected modified surfaces	1244:1269	The capacity of tetracycline sorption and release by selected modified surfaces were followed and compared to the untreated NWM.
34965089	2	3	theme	[poly	506:510	arg1	presence					483:490	the presence	479:490	the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid]	479:683	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	3	theme	[poly	506:510	arg1	complexes					722:730	nonstoichiometric polyelectrolyte complexes	688:730	nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces	688:846	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	4	theme	mol	610:612	arg1	acid					653:656	55 mol % 2-acrylamido-2-methylpropanesulfonic acid	607:656	55 mol % 2-acrylamido-2-methylpropanesulfonic acid	607:656	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	1	5	theme	cheap	289:293	arg1	material					354:361	a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material	287:361	a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM)	287:367	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	1	5	theme	cheap	289:293	arg1	substrate					376:384	the substrate	372:384	the substrate	372:384	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	1	5	theme	cheap	289:293	arg1	NWM					364:366	NWM	364:366	NWM	364:366	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	6	6	theme	antifouling	1425:1435	arg1	capability					1437:1446	antifouling capability	1425:1446	antifouling capability	1425:1446	Also, the biological properties were evaluated in terms of biocompatibility, antibacterial activity, and antifouling capability.
34965089	2	7	theme	nonstoichiometric	688:704	arg1	complexes					722:730	nonstoichiometric polyelectrolyte complexes	688:730	nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces	688:846	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	8	theme	55	607:608	arg1	mol					610:612	mol	610:612	mol	610:612	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	0	9	with	Patterns	89:96	arg1	Applications					114:125	Biomedical Applications	103:125	Biomedical Applications	103:125	Hydrophobic Composites Designed by a Nonwoven Cellulose-Based Material and Polymer/CaCO3 Patterns with Biomedical Applications.
34965089	0	10	theme	Biomedical	103:112	arg1	Applications					114:125	Biomedical Applications	103:125	Biomedical Applications	103:125	Hydrophobic Composites Designed by a Nonwoven Cellulose-Based Material and Polymer/CaCO3 Patterns with Biomedical Applications.
34965089	2	11	theme	%	669:669	arg1	presence					483:490	the presence	479:490	the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid]	479:683	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	11	theme	%	669:669	arg1	complexes					722:730	nonstoichiometric polyelectrolyte complexes	688:730	nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces	688:846	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	4	12	theme	110-135°	1181:1188	arg1	range					1172:1176	the range	1168:1176	the range of 110-135°	1168:1188	The obtained composite materials become hydrophobic, having a contact angle in the range of 110-135°.
34965089	1	13	theme	versatile	296:304	arg1	material					354:361	a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material	287:361	a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM)	287:367	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	1	13	theme	versatile	296:304	arg1	substrate					376:384	the substrate	372:384	the substrate	372:384	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	1	13	theme	versatile	296:304	arg1	NWM					364:366	NWM	364:366	NWM	364:366	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	4	14	theme	contact	1151:1157	arg1	angle					1159:1163	a contact angle	1149:1163	a contact angle	1149:1163	The obtained composite materials become hydrophobic, having a contact angle in the range of 110-135°.
34965089	2	15	theme	45	662:663	arg1	mol					665:667	mol	665:667	mol	665:667	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	16	theme	mol	665:667	arg1	%					669:669	45 mol %	662:669	45 mol %	662:669	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	17	from	[poly	749:753	arg1	surfaces					839:846	polycation-treated surfaces	820:846	polycation-treated surfaces	820:846	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	17	from	[poly	749:753	arg1	NWM					809:811	a pristine NWM	798:811	a pristine NWM	798:811	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	1	18	theme	hydrophobic	172:182	arg1	surfaces					184:191	hydrophobic surfaces	172:191	hydrophobic surfaces	172:191	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	3	19	theme	surface	853:859	arg1	morphology					861:870	The surface morphology	849:870	The surface morphology obtained by calcite growth under surface or environmental functional groups' influence and the hydrophilic/hydrophobic character of the composite materials	849:1026	The surface morphology obtained by calcite growth under surface or environmental functional groups' influence and the hydrophilic/hydrophobic character of the composite materials were followed and compared to that of the starting material.
34965089	2	20	theme	polyanions	495:504	arg1	acid					512:515	acid acrylic	512:523	acid acrylic	512:523	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	20	theme	polyanions	495:504	arg1	[poly					506:510	polyanions [poly	495:510	polyanions [poly(acid acrylic)	495:524	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	21	theme	polyelectrolyte	706:720	arg1	complexes					722:730	nonstoichiometric polyelectrolyte complexes	688:730	nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces	688:846	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	22	with	complexes	722:730	arg1	[poly					749:753	polycations [poly	737:753	polycations [poly(allylamine hydrochloride)	737:779	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	22	with	complexes	722:730	arg1	hydrochloride					766:778	allylamine hydrochloride	755:778	allylamine hydrochloride	755:778	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	22	with	complexes	722:730	arg1	chitosan					785:792	chitosan	785:792	chitosan	785:792	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	5	23	theme	selected	1244:1251	arg1	surfaces					1262:1269	selected modified surfaces	1244:1269	selected modified surfaces	1244:1269	The capacity of tetracycline sorption and release by selected modified surfaces were followed and compared to the untreated NWM.
34965089	2	24	theme	acrylic	517:523	arg1	acid					512:515	acid acrylic	512:523	acid acrylic	512:523	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	24	theme	acrylic	517:523	arg1	[poly					506:510	polyanions [poly	495:510	polyanions [poly(acid acrylic)	495:524	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	25	dep	[poly	506:510	arg1	acid					679:682	acrylic acid	671:682	acrylic acid	671:682	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	26	theme	crystal	408:414	arg1	growth					416:421	the CaCO3 crystal growth	398:421	the CaCO3 crystal growth	398:421	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	0	27	theme	Hydrophobic	0:10	arg1	Composites					12:21	Hydrophobic Composites	0:21	Hydrophobic Composites	0:21	Hydrophobic Composites Designed by a Nonwoven Cellulose-Based Material and Polymer/CaCO3 Patterns with Biomedical Applications.
34965089	2	28	theme	2-acrylamido-2-methylpropanesulfonic	616:651	arg1	acid					653:656	55 mol % 2-acrylamido-2-methylpropanesulfonic acid	607:656	55 mol % 2-acrylamido-2-methylpropanesulfonic acid	607:656	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	1	29	theme	surface	196:202	arg1	modification					204:215	surface modification	196:215	surface modification with calcium carbonate	196:238	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	4	30	theme	obtained	1093:1100	arg1	materials					1112:1120	The obtained composite materials	1089:1120	The obtained composite materials	1089:1120	The obtained composite materials become hydrophobic, having a contact angle in the range of 110-135°.
34965089	5	31	theme	release	1233:1239	arg1	capacity					1195:1202	The capacity	1191:1202	The capacity of tetracycline sorption and release by selected modified surfaces	1191:1269	The capacity of tetracycline sorption and release by selected modified surfaces were followed and compared to the untreated NWM.
34965089	6	32	theme	antibacterial	1397:1409	arg1	activity					1411:1418	antibacterial activity	1397:1418	antibacterial activity	1397:1418	Also, the biological properties were evaluated in terms of biocompatibility, antibacterial activity, and antifouling capability.
34965089	6	33	theme	capability	1437:1446	arg1	terms					1370:1374	terms	1370:1374	terms of biocompatibility, antibacterial activity, and antifouling capability	1370:1446	Also, the biological properties were evaluated in terms of biocompatibility, antibacterial activity, and antifouling capability.
34965089	2	34	theme	diffusion	447:455	arg1	method					457:462	the ammonium carbonate diffusion method	424:462	the ammonium carbonate diffusion method	424:462	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	1	35	theme	super-hydrophilic	311:327	arg1	material					354:361	a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material	287:361	a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM)	287:367	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	1	35	theme	super-hydrophilic	311:327	arg1	substrate					376:384	the substrate	372:384	the substrate	372:384	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	1	35	theme	super-hydrophilic	311:327	arg1	NWM					364:366	NWM	364:366	NWM	364:366	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	3	36	theme	hydrophilic/hydrophobic	967:989	arg1	character					991:999	the hydrophilic/hydrophobic character	963:999	the hydrophilic/hydrophobic character of the composite materials	963:1026	The surface morphology obtained by calcite growth under surface or environmental functional groups' influence and the hydrophilic/hydrophobic character of the composite materials were followed and compared to that of the starting material.
34965089	2	37	theme	pristine	800:807	arg1	NWM					809:811	a pristine NWM	798:811	a pristine NWM	798:811	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	38	theme	carbonate	437:445	arg1	method					457:462	the ammonium carbonate diffusion method	424:462	the ammonium carbonate diffusion method	424:462	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	0	39	theme	Nonwoven	37:44	arg1	Material					62:69	a Nonwoven Cellulose-Based Material	35:69	a Nonwoven Cellulose-Based Material	35:69	Hydrophobic Composites Designed by a Nonwoven Cellulose-Based Material and Polymer/CaCO3 Patterns with Biomedical Applications.
34965089	1	40	theme	cellulose-based	329:343	arg1	material					354:361	a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material	287:361	a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM)	287:367	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	1	40	theme	cellulose-based	329:343	arg1	substrate					376:384	the substrate	372:384	the substrate	372:384	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	1	40	theme	cellulose-based	329:343	arg1	NWM					364:366	NWM	364:366	NWM	364:366	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	1	41	with	modification	204:215	arg1	carbonate					230:238	calcium carbonate	222:238	calcium carbonate	222:238	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	1	42	theme	calcium	222:228	arg1	carbonate					230:238	calcium carbonate	222:238	calcium carbonate	222:238	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	2	43	from	chitosan	785:792	arg1	surfaces					839:846	polycation-treated surfaces	820:846	polycation-treated surfaces	820:846	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	43	from	chitosan	785:792	arg1	NWM					809:811	a pristine NWM	798:811	a pristine NWM	798:811	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	44	theme	CaCO3	402:406	arg1	growth					416:421	the CaCO3 crystal growth	398:421	the CaCO3 crystal growth	398:421	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	45	theme	ammonium	428:435	arg1	method					457:462	the ammonium carbonate diffusion method	424:462	the ammonium carbonate diffusion method	424:462	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	1	46	theme	nonwoven	345:352	arg1	material					354:361	a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material	287:361	a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM)	287:367	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	1	46	theme	nonwoven	345:352	arg1	substrate					376:384	the substrate	372:384	the substrate	372:384	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	1	46	theme	nonwoven	345:352	arg1	NWM					364:366	NWM	364:366	NWM	364:366	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	2	47	theme	2-acrylamido-2-methylpropanesulfonic	532:567	arg1	poly					527:530	poly	527:530	poly(2-acrylamido-2-methylpropanesulfonic acid)	527:573	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	47	theme	2-acrylamido-2-methylpropanesulfonic	532:567	arg1	acid					569:572	2-acrylamido-2-methylpropanesulfonic acid	532:572	2-acrylamido-2-methylpropanesulfonic acid	532:572	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	6	48	theme	biocompatibility	1379:1394	arg1	terms					1370:1374	terms	1370:1374	terms of biocompatibility, antibacterial activity, and antifouling capability	1370:1446	Also, the biological properties were evaluated in terms of biocompatibility, antibacterial activity, and antifouling capability.
34965089	3	49	theme	materials	1018:1026	arg1	influence					949:957	surface or environmental functional groups' influence	905:957	surface or environmental functional groups' influence	905:957	The surface morphology obtained by calcite growth under surface or environmental functional groups' influence and the hydrophilic/hydrophobic character of the composite materials were followed and compared to that of the starting material.
34965089	3	49	theme	materials	1018:1026	arg1	character					991:999	the hydrophilic/hydrophobic character	963:999	the hydrophilic/hydrophobic character of the composite materials	963:1026	The surface morphology obtained by calcite growth under surface or environmental functional groups' influence and the hydrophilic/hydrophobic character of the composite materials were followed and compared to that of the starting material.
34965089	6	50	theme	activity	1411:1418	arg1	terms					1370:1374	terms	1370:1374	terms of biocompatibility, antibacterial activity, and antifouling capability	1370:1446	Also, the biological properties were evaluated in terms of biocompatibility, antibacterial activity, and antifouling capability.
34965089	2	51	theme	polycations	737:747	arg1	[poly					749:753	polycations [poly	737:753	polycations [poly(allylamine hydrochloride)	737:779	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	51	theme	polycations	737:747	arg1	hydrochloride					766:778	allylamine hydrochloride	755:778	allylamine hydrochloride	755:778	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	0	52	theme	Cellulose-Based	46:60	arg1	Material					62:69	a Nonwoven Cellulose-Based Material	35:69	a Nonwoven Cellulose-Based Material	35:69	Hydrophobic Composites Designed by a Nonwoven Cellulose-Based Material and Polymer/CaCO3 Patterns with Biomedical Applications.
34965089	5	53	theme	tetracycline	1207:1218	arg1	sorption					1220:1227	tetracycline sorption and release	1207:1239	sorption	1220:1227	The capacity of tetracycline sorption and release by selected modified surfaces were followed and compared to the untreated NWM.
34965089	2	54	theme	copolymer	582:590	arg1	presence					483:490	the presence	479:490	the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid]	479:683	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	54	theme	copolymer	582:590	arg1	complexes					722:730	nonstoichiometric polyelectrolyte complexes	688:730	nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces	688:846	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	55	contain	contains	598:605	arg2	acid					653:656	55 mol % 2-acrylamido-2-methylpropanesulfonic acid	607:656	55 mol % 2-acrylamido-2-methylpropanesulfonic acid	607:656	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	55	contain	contains	598:605	arg1	copolymer					582:590	a copolymer	580:590	a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid	580:656	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	5	56	theme	sorption	1220:1227	arg1	capacity					1195:1202	The capacity	1191:1202	The capacity of tetracycline sorption and release by selected modified surfaces	1191:1269	The capacity of tetracycline sorption and release by selected modified surfaces were followed and compared to the untreated NWM.
34965089	6	57	theme	biological	1330:1339	arg1	properties					1341:1350	the biological properties	1326:1350	the biological properties	1326:1350	Also, the biological properties were evaluated in terms of biocompatibility, antibacterial activity, and antifouling capability.
34965089	2	58	theme	acrylic	671:677	arg1	acid					679:682	acrylic acid	671:682	acrylic acid	671:682	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	3	59	theme	composite	1008:1016	arg1	materials					1018:1026	the composite materials	1004:1026	the composite materials	1004:1026	The surface morphology obtained by calcite growth under surface or environmental functional groups' influence and the hydrophilic/hydrophobic character of the composite materials were followed and compared to that of the starting material.
34965089	4	60	theme	composite	1102:1110	arg1	materials					1112:1120	The obtained composite materials	1089:1120	The obtained composite materials	1089:1120	The obtained composite materials become hydrophobic, having a contact angle in the range of 110-135°.
34965089	2	61	with	presence	483:490	arg1	[poly					749:753	polycations [poly	737:753	polycations [poly(allylamine hydrochloride)	737:779	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	61	with	presence	483:490	arg1	hydrochloride					766:778	allylamine hydrochloride	755:778	allylamine hydrochloride	755:778	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	61	with	presence	483:490	arg1	chitosan					785:792	chitosan	785:792	chitosan	785:792	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	0	62	with	Material	62:69	arg1	Applications					114:125	Biomedical Applications	103:125	Biomedical Applications	103:125	Hydrophobic Composites Designed by a Nonwoven Cellulose-Based Material and Polymer/CaCO3 Patterns with Biomedical Applications.
34965089	2	63	theme	polycation-treated	820:837	arg1	surfaces					839:846	polycation-treated surfaces	820:846	polycation-treated surfaces	820:846	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	1	64	theme	diffusion-controlled	244:263	arg1	crystallization					265:279	diffusion-controlled crystallization	244:279	diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate	244:384	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	0	65	theme	Polymer/CaCO3	75:87	arg1	Patterns					89:96	Polymer/CaCO3 Patterns	75:96	Polymer/CaCO3 Patterns	75:96	Hydrophobic Composites Designed by a Nonwoven Cellulose-Based Material and Polymer/CaCO3 Patterns with Biomedical Applications.
34965089	3	66	theme	starting	1070:1077	arg1	material					1079:1086	the starting material	1066:1086	the starting material	1066:1086	The surface morphology obtained by calcite growth under surface or environmental functional groups' influence and the hydrophilic/hydrophobic character of the composite materials were followed and compared to that of the starting material.
34965089	5	67	theme	untreated	1305:1313	arg1	NWM					1315:1317	the untreated NWM	1301:1317	the untreated NWM	1301:1317	The capacity of tetracycline sorption and release by selected modified surfaces were followed and compared to the untreated NWM.
34965089	1	68	theme	simple	148:153	arg1	method					155:160	a simple method	146:160	a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate	146:384	Herein, we report a simple method to obtain hydrophobic surfaces by surface modification with calcium carbonate via diffusion-controlled crystallization using a cheap, versatile, and super-hydrophilic cellulose-based nonwoven material (NWM) as the substrate.
34965089	3	69	theme	calcite	884:890	arg1	growth					892:897	calcite growth	884:897	calcite growth	884:897	The surface morphology obtained by calcite growth under surface or environmental functional groups' influence and the hydrophilic/hydrophobic character of the composite materials were followed and compared to that of the starting material.
34965089	2	70	theme	allylamine	755:764	arg1	[poly					749:753	polycations [poly	737:753	polycations [poly(allylamine hydrochloride)	737:779	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	70	theme	allylamine	755:764	arg1	hydrochloride					766:778	allylamine hydrochloride	755:778	allylamine hydrochloride	755:778	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	3	71	theme	environmental	916:928	arg1	groups					941:946	environmental functional groups	916:946	environmental functional groups	916:946	The surface morphology obtained by calcite growth under surface or environmental functional groups' influence and the hydrophilic/hydrophobic character of the composite materials were followed and compared to that of the starting material.
34965089	2	72	theme	poly	527:530	arg1	presence					483:490	the presence	479:490	the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid]	479:683	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
34965089	2	72	theme	poly	527:530	arg1	complexes					722:730	nonstoichiometric polyelectrolyte complexes	688:730	nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces	688:846	To control the CaCO3 crystal growth, the ammonium carbonate diffusion method was applied in the presence of polyanions [poly(acid acrylic), poly(2-acrylamido-2-methylpropanesulfonic acid), and a copolymer which contains 55 mol % 2-acrylamido-2-methylpropanesulfonic acid and 45 mol % acrylic acid] or nonstoichiometric polyelectrolyte complexes with polycations [poly(allylamine hydrochloride) and chitosan] on a pristine NWM and on polycation-treated surfaces.
33142606	3	0	dep	based	406:410	arg1	followed					577:584	followed	577:584	followed by the in situ ionic-crosslinking	577:618	The strategy was based on the incorporation of alginate materials with distinctive anisotropic features (fibers, fabrics and aerogels) into the precursor solution of congeneric hydrogels, followed by the in situ ionic-crosslinking.
33142606	3	1	theme	precursor	533:541	arg1	solution					543:550	the precursor solution	529:550	the precursor solution of congeneric hydrogels	529:574	The strategy was based on the incorporation of alginate materials with distinctive anisotropic features (fibers, fabrics and aerogels) into the precursor solution of congeneric hydrogels, followed by the in situ ionic-crosslinking.
33142606	1	2	link	Natural-derived	105:119	arg1	hydrogels					121:129	Natural-derived hydrogels	105:129	Natural-derived hydrogels	105:129	Natural-derived hydrogels are expected as promising structural biomaterials, but the soft character severely limits their applications.
33142606	1	2	link	Natural-derived	105:119	arg1	biomaterials					168:179	promising structural biomaterials	147:179	promising structural biomaterials	147:179	Natural-derived hydrogels are expected as promising structural biomaterials, but the soft character severely limits their applications.
33142606	6	3	theme	extra	1156:1160	arg1	agents					1171:1176	extra coupling agents	1156:1176	extra coupling agents	1156:1176	This research offers a new path to design the interface of polysaccharide composites without extra coupling agents.
33142606	6	4	theme	coupling	1162:1169	arg1	agents					1171:1176	extra coupling agents	1156:1176	extra coupling agents	1156:1176	This research offers a new path to design the interface of polysaccharide composites without extra coupling agents.
33142606	3	5	dep	in	593:594	arg1	situ					596:599	situ	596:599	situ	596:599	The strategy was based on the incorporation of alginate materials with distinctive anisotropic features (fibers, fabrics and aerogels) into the precursor solution of congeneric hydrogels, followed by the in situ ionic-crosslinking.
33142606	3	6	theme	in	593:594	arg1	ionic-crosslinking					601:618	the in situ ionic-crosslinking	589:618	the in situ ionic-crosslinking	589:618	The strategy was based on the incorporation of alginate materials with distinctive anisotropic features (fibers, fabrics and aerogels) into the precursor solution of congeneric hydrogels, followed by the in situ ionic-crosslinking.
33142606	4	7	theme	phase	818:822	arg1	matrix					837:842	phase and hydrogel matrix	818:842	matrix	837:842	Interestingly, triggered by the concentration difference, the cations-Ca2+ in reinforcing phase could diffuse into the interface and simultaneously chelate with alginate chains of both reinforcing phase and hydrogel matrix, acting as self-generating interfacial binders.
33142606	2	8	theme	super-strong	306:317	arg1	hydrogels					348:356	super-strong and tough alginate composite hydrogels	306:356	super-strong and tough alginate composite hydrogels	306:356	Here, a facile yet effective strategy was developed to fabricate super-strong and tough alginate composite hydrogels via a self-reinforcing method.
33142606	3	9	theme	hydrogels	566:574	arg1	solution					543:550	the precursor solution	529:550	the precursor solution of congeneric hydrogels	529:574	The strategy was based on the incorporation of alginate materials with distinctive anisotropic features (fibers, fabrics and aerogels) into the precursor solution of congeneric hydrogels, followed by the in situ ionic-crosslinking.
33142606	6	10	theme	polysaccharide	1122:1135	arg1	composites					1137:1146	polysaccharide composites	1122:1146	polysaccharide composites without extra coupling agents	1122:1176	This research offers a new path to design the interface of polysaccharide composites without extra coupling agents.
33142606	1	11	theme	soft	190:193	arg1	character					195:203	the soft character	186:203	the soft character	186:203	Natural-derived hydrogels are expected as promising structural biomaterials, but the soft character severely limits their applications.
33142606	0	12	theme	intimate	40:47	arg1	interface					49:57	the intimate interface	36:57	the intimate interface for anisotropic alginate composite hydrogels	36:102	A self-reinforcing strategy enables the intimate interface for anisotropic alginate composite hydrogels.
33142606	4	13	theme	hydrogel	828:835	arg1	matrix					837:842	phase and hydrogel matrix	818:842	matrix	837:842	Interestingly, triggered by the concentration difference, the cations-Ca2+ in reinforcing phase could diffuse into the interface and simultaneously chelate with alginate chains of both reinforcing phase and hydrogel matrix, acting as self-generating interfacial binders.
33142606	4	14	theme	reinforcing	699:709	arg1	phase					711:715	reinforcing phase	699:715	reinforcing phase	699:715	Interestingly, triggered by the concentration difference, the cations-Ca2+ in reinforcing phase could diffuse into the interface and simultaneously chelate with alginate chains of both reinforcing phase and hydrogel matrix, acting as self-generating interfacial binders.
33142606	0	15	theme	self-reinforcing	2:17	arg1	strategy					19:26	A self-reinforcing strategy	0:26	A self-reinforcing strategy	0:26	A self-reinforcing strategy enables the intimate interface for anisotropic alginate composite hydrogels.
33142606	0	16	theme	composite	84:92	arg1	hydrogels					94:102	anisotropic alginate composite hydrogels	63:102	anisotropic alginate composite hydrogels	63:102	A self-reinforcing strategy enables the intimate interface for anisotropic alginate composite hydrogels.
33142606	3	17	theme	distinctive	460:470	arg1	features					484:491	distinctive anisotropic features	460:491	distinctive anisotropic features (fibers, fabrics and aerogels)	460:522	The strategy was based on the incorporation of alginate materials with distinctive anisotropic features (fibers, fabrics and aerogels) into the precursor solution of congeneric hydrogels, followed by the in situ ionic-crosslinking.
33142606	6	18	theme	new	1086:1088	arg1	path					1090:1093	a new path	1084:1093	a new path to design the interface of polysaccharide composites without extra coupling agents	1084:1176	This research offers a new path to design the interface of polysaccharide composites without extra coupling agents.
33142606	1	19	theme	Natural-derived	105:119	arg1	hydrogels					121:129	Natural-derived hydrogels	105:129	Natural-derived hydrogels	105:129	Natural-derived hydrogels are expected as promising structural biomaterials, but the soft character severely limits their applications.
33142606	1	19	theme	Natural-derived	105:119	arg1	biomaterials					168:179	promising structural biomaterials	147:179	promising structural biomaterials	147:179	Natural-derived hydrogels are expected as promising structural biomaterials, but the soft character severely limits their applications.
33142606	3	20	theme	anisotropic	472:482	arg1	features					484:491	distinctive anisotropic features	460:491	distinctive anisotropic features (fibers, fabrics and aerogels)	460:522	The strategy was based on the incorporation of alginate materials with distinctive anisotropic features (fibers, fabrics and aerogels) into the precursor solution of congeneric hydrogels, followed by the in situ ionic-crosslinking.
33142606	2	21	theme	alginate	329:336	arg1	hydrogels					348:356	super-strong and tough alginate composite hydrogels	306:356	super-strong and tough alginate composite hydrogels	306:356	Here, a facile yet effective strategy was developed to fabricate super-strong and tough alginate composite hydrogels via a self-reinforcing method.
33142606	4	22	from	cations-Ca2+	683:694	arg1	phase					711:715	reinforcing phase	699:715	reinforcing phase	699:715	Interestingly, triggered by the concentration difference, the cations-Ca2+ in reinforcing phase could diffuse into the interface and simultaneously chelate with alginate chains of both reinforcing phase and hydrogel matrix, acting as self-generating interfacial binders.
33142606	2	23	theme	composite	338:346	arg1	hydrogels					348:356	super-strong and tough alginate composite hydrogels	306:356	super-strong and tough alginate composite hydrogels	306:356	Here, a facile yet effective strategy was developed to fabricate super-strong and tough alginate composite hydrogels via a self-reinforcing method.
33142606	3	24	with	materials	445:453	arg1	features					484:491	distinctive anisotropic features	460:491	distinctive anisotropic features (fibers, fabrics and aerogels)	460:522	The strategy was based on the incorporation of alginate materials with distinctive anisotropic features (fibers, fabrics and aerogels) into the precursor solution of congeneric hydrogels, followed by the in situ ionic-crosslinking.
33142606	2	25	theme	tough	323:327	arg1	hydrogels					348:356	super-strong and tough alginate composite hydrogels	306:356	super-strong and tough alginate composite hydrogels	306:356	Here, a facile yet effective strategy was developed to fabricate super-strong and tough alginate composite hydrogels via a self-reinforcing method.
33142606	3	26	theme	materials	445:453	arg1	incorporation					419:431	the incorporation	415:431	the incorporation of alginate materials with distinctive anisotropic features (fibers, fabrics and aerogels) into the precursor solution of congeneric hydrogels	415:574	The strategy was based on the incorporation of alginate materials with distinctive anisotropic features (fibers, fabrics and aerogels) into the precursor solution of congeneric hydrogels, followed by the in situ ionic-crosslinking.
33142606	5	27	theme	reinforcing	983:993	arg1	phase					995:999	the rigid reinforcing phase	973:999	the rigid reinforcing phase	973:999	Contributed by the intimate interface, the load was effectively transferred into the rigid reinforcing phase, and the hydrogels integrated them into a mechanical network.
33142606	5	28	theme	intimate	911:918	arg1	interface					920:928	the intimate interface	907:928	the intimate interface	907:928	Contributed by the intimate interface, the load was effectively transferred into the rigid reinforcing phase, and the hydrogels integrated them into a mechanical network.
33142606	2	29	theme	facile	249:254	arg1	strategy					270:277	a facile yet effective strategy	247:277	a facile yet effective strategy	247:277	Here, a facile yet effective strategy was developed to fabricate super-strong and tough alginate composite hydrogels via a self-reinforcing method.
33142606	3	30	dep	features	484:491	arg1	aerogels					514:521	aerogels	514:521	aerogels	514:521	The strategy was based on the incorporation of alginate materials with distinctive anisotropic features (fibers, fabrics and aerogels) into the precursor solution of congeneric hydrogels, followed by the in situ ionic-crosslinking.
33142606	3	30	dep	features	484:491	arg1	fabrics					502:508	fabrics	502:508	fabrics	502:508	The strategy was based on the incorporation of alginate materials with distinctive anisotropic features (fibers, fabrics and aerogels) into the precursor solution of congeneric hydrogels, followed by the in situ ionic-crosslinking.
33142606	3	30	dep	features	484:491	arg1	fibers					494:499	fibers	494:499	fibers	494:499	The strategy was based on the incorporation of alginate materials with distinctive anisotropic features (fibers, fabrics and aerogels) into the precursor solution of congeneric hydrogels, followed by the in situ ionic-crosslinking.
33142606	5	31	theme	rigid	977:981	arg1	phase					995:999	the rigid reinforcing phase	973:999	the rigid reinforcing phase	973:999	Contributed by the intimate interface, the load was effectively transferred into the rigid reinforcing phase, and the hydrogels integrated them into a mechanical network.
33142606	3	32	theme	alginate	436:443	arg1	materials					445:453	alginate materials	436:453	alginate materials with distinctive anisotropic features (fibers, fabrics and aerogels)	436:522	The strategy was based on the incorporation of alginate materials with distinctive anisotropic features (fibers, fabrics and aerogels) into the precursor solution of congeneric hydrogels, followed by the in situ ionic-crosslinking.
33142606	2	33	theme	self-reinforcing	364:379	arg1	method					381:386	a self-reinforcing method	362:386	a self-reinforcing method	362:386	Here, a facile yet effective strategy was developed to fabricate super-strong and tough alginate composite hydrogels via a self-reinforcing method.
33142606	4	34	theme	interfacial	871:881	arg1	binders					883:889	self-generating interfacial binders	855:889	self-generating interfacial binders	855:889	Interestingly, triggered by the concentration difference, the cations-Ca2+ in reinforcing phase could diffuse into the interface and simultaneously chelate with alginate chains of both reinforcing phase and hydrogel matrix, acting as self-generating interfacial binders.
33142606	1	35	theme	promising	147:155	arg1	hydrogels					121:129	Natural-derived hydrogels	105:129	Natural-derived hydrogels	105:129	Natural-derived hydrogels are expected as promising structural biomaterials, but the soft character severely limits their applications.
33142606	1	35	theme	promising	147:155	arg1	biomaterials					168:179	promising structural biomaterials	147:179	promising structural biomaterials	147:179	Natural-derived hydrogels are expected as promising structural biomaterials, but the soft character severely limits their applications.
33142606	5	36	theme	mechanical	1043:1052	arg1	network					1054:1060	a mechanical network	1041:1060	a mechanical network	1041:1060	Contributed by the intimate interface, the load was effectively transferred into the rigid reinforcing phase, and the hydrogels integrated them into a mechanical network.
33142606	4	37	theme	both	801:804	arg1	chains					791:796	alginate chains	782:796	alginate chains	782:796	Interestingly, triggered by the concentration difference, the cations-Ca2+ in reinforcing phase could diffuse into the interface and simultaneously chelate with alginate chains of both reinforcing phase and hydrogel matrix, acting as self-generating interfacial binders.
33142606	1	38	theme	structural	157:166	arg1	hydrogels					121:129	Natural-derived hydrogels	105:129	Natural-derived hydrogels	105:129	Natural-derived hydrogels are expected as promising structural biomaterials, but the soft character severely limits their applications.
33142606	1	38	theme	structural	157:166	arg1	biomaterials					168:179	promising structural biomaterials	147:179	promising structural biomaterials	147:179	Natural-derived hydrogels are expected as promising structural biomaterials, but the soft character severely limits their applications.
33142606	4	39	theme	alginate	782:789	arg1	chains					791:796	alginate chains	782:796	alginate chains	782:796	Interestingly, triggered by the concentration difference, the cations-Ca2+ in reinforcing phase could diffuse into the interface and simultaneously chelate with alginate chains of both reinforcing phase and hydrogel matrix, acting as self-generating interfacial binders.
33142606	0	40	theme	alginate	75:82	arg1	hydrogels					94:102	anisotropic alginate composite hydrogels	63:102	anisotropic alginate composite hydrogels	63:102	A self-reinforcing strategy enables the intimate interface for anisotropic alginate composite hydrogels.
33142606	4	41	theme	self-generating	855:869	arg1	binders					883:889	self-generating interfacial binders	855:889	self-generating interfacial binders	855:889	Interestingly, triggered by the concentration difference, the cations-Ca2+ in reinforcing phase could diffuse into the interface and simultaneously chelate with alginate chains of both reinforcing phase and hydrogel matrix, acting as self-generating interfacial binders.
33142606	6	42	theme	composites	1137:1146	arg1	interface					1109:1117	the interface	1105:1117	the interface of polysaccharide composites without extra coupling agents	1105:1176	This research offers a new path to design the interface of polysaccharide composites without extra coupling agents.
33142606	0	43	theme	anisotropic	63:73	arg1	hydrogels					94:102	anisotropic alginate composite hydrogels	63:102	anisotropic alginate composite hydrogels	63:102	A self-reinforcing strategy enables the intimate interface for anisotropic alginate composite hydrogels.
33142606	2	44	theme	effective	260:268	arg1	strategy					270:277	a facile yet effective strategy	247:277	a facile yet effective strategy	247:277	Here, a facile yet effective strategy was developed to fabricate super-strong and tough alginate composite hydrogels via a self-reinforcing method.
33142606	3	45	theme	congeneric	555:564	arg1	hydrogels					566:574	congeneric hydrogels	555:574	congeneric hydrogels	555:574	The strategy was based on the incorporation of alginate materials with distinctive anisotropic features (fibers, fabrics and aerogels) into the precursor solution of congeneric hydrogels, followed by the in situ ionic-crosslinking.
33142606	4	46	theme	concentration	653:665	arg1	difference					667:676	the concentration difference	649:676	the concentration difference	649:676	Interestingly, triggered by the concentration difference, the cations-Ca2+ in reinforcing phase could diffuse into the interface and simultaneously chelate with alginate chains of both reinforcing phase and hydrogel matrix, acting as self-generating interfacial binders.
33151676	6	0	theme	stacking	932:939	arg1	interaction					941:951	The strong stacking interaction	921:951	The strong stacking interaction of compound 6p with Trp99 and Trp220 found in the SmChiB-6p co-crystal structure	921:1032	The strong stacking interaction of compound 6p with Trp99 and Trp220 found in the SmChiB-6p co-crystal structure verifies the feasibility of our design.
33151676	2	1	theme	therapeutic	368:378	arg1	drugs					380:384	therapeutic drugs	368:384	therapeutic drugs	368:384	Thus, chitinases are attractive targets for therapeutic drugs and pesticides.
33151676	6	2	theme	co-crystal	1013:1022	arg1	structure					1024:1032	the SmChiB-6p co-crystal structure	999:1032	the SmChiB-6p co-crystal structure	999:1032	The strong stacking interaction of compound 6p with Trp99 and Trp220 found in the SmChiB-6p co-crystal structure verifies the feasibility of our design.
33151676	0	3	theme	Inhibitors	74:83	arg1	Discovery					45:53	Molecular Docking Guided Discovery	20:53	Molecular Docking Guided Discovery	20:53	X-ray Structure and Molecular Docking Guided Discovery of Novel Chitinase Inhibitors with a Scaffold of Dipyridopyrimidine-3-carboxamide.
33151676	0	3	theme	Inhibitors	74:83	arg1	Structure					6:14	X-ray Structure	0:14	X-ray Structure	0:14	X-ray Structure and Molecular Docking Guided Discovery of Novel Chitinase Inhibitors with a Scaffold of Dipyridopyrimidine-3-carboxamide.
33151676	6	4	theme	strong	925:930	arg1	interaction					941:951	The strong stacking interaction	921:951	The strong stacking interaction of compound 6p with Trp99 and Trp220 found in the SmChiB-6p co-crystal structure	921:1032	The strong stacking interaction of compound 6p with Trp99 and Trp220 found in the SmChiB-6p co-crystal structure verifies the feasibility of our design.
33151676	3	5	theme	heterocycles	516:527	arg1	construction					493:504	the construction	489:504	the construction of planar heterocycles	489:527	Here, we present a strategy of developing a novel chemotype of chitinase inhibitors by the construction of planar heterocycles that can stack with conserved aromatic residues.
33151676	1	6	theme	fungal	275:280	arg1	remodeling					292:301	fungal cell wall remodeling	275:301	fungal cell wall remodeling	275:301	Chitinases are the glycosyl hydrolase for catalyzing the degradation of chitin and play an indispensable role in bacterial pathogenesis, fungal cell wall remodeling, and insect molting.
33151676	5	7	theme	compound	759:766	arg1	6t					768:769	compound 6t	759:769	compound 6t	759:769	Among them, compound 6t showed the most potent activity against bacterial chitinase SmChiB and insect chitinase OfChi-h, with a Ki value of 0.14 and 0.0056 μM, respectively.
33151676	7	8	theme	chitinase	1132:1140	arg1	inhibitors					1142:1151	potent chitinase inhibitors	1125:1151	potent chitinase inhibitors for pathogen and pest control	1125:1181	Our results provide novel insights into developing potent chitinase inhibitors for pathogen and pest control.
33151676	3	9	theme	conserved	549:557	arg1	residues					568:575	conserved aromatic residues	549:575	conserved aromatic residues	549:575	Here, we present a strategy of developing a novel chemotype of chitinase inhibitors by the construction of planar heterocycles that can stack with conserved aromatic residues.
33151676	1	10	theme	cell	282:285	arg1	remodeling					292:301	fungal cell wall remodeling	275:301	fungal cell wall remodeling	275:301	Chitinases are the glycosyl hydrolase for catalyzing the degradation of chitin and play an indispensable role in bacterial pathogenesis, fungal cell wall remodeling, and insect molting.
33151676	3	11	theme	aromatic	559:566	arg1	residues					568:575	conserved aromatic residues	549:575	conserved aromatic residues	549:575	Here, we present a strategy of developing a novel chemotype of chitinase inhibitors by the construction of planar heterocycles that can stack with conserved aromatic residues.
33151676	3	12	theme	planar	509:514	arg1	heterocycles					516:527	planar heterocycles	509:527	planar heterocycles	509:527	Here, we present a strategy of developing a novel chemotype of chitinase inhibitors by the construction of planar heterocycles that can stack with conserved aromatic residues.
33151676	1	13	theme	wall	287:290	arg1	remodeling					292:301	fungal cell wall remodeling	275:301	fungal cell wall remodeling	275:301	Chitinases are the glycosyl hydrolase for catalyzing the degradation of chitin and play an indispensable role in bacterial pathogenesis, fungal cell wall remodeling, and insect molting.
33151676	0	14	theme	Dipyridopyrimidine-3-carboxamide	104:135	arg1	Scaffold					92:99	a Scaffold	90:99	a Scaffold of Dipyridopyrimidine-3-carboxamide	90:135	X-ray Structure and Molecular Docking Guided Discovery of Novel Chitinase Inhibitors with a Scaffold of Dipyridopyrimidine-3-carboxamide.
33151676	6	15	theme	design	1066:1071	arg1	feasibility					1047:1057	the feasibility	1043:1057	the feasibility of our design	1043:1071	The strong stacking interaction of compound 6p with Trp99 and Trp220 found in the SmChiB-6p co-crystal structure verifies the feasibility of our design.
33151676	5	16	theme	bacterial	811:819	arg1	SmChiB					831:836	bacterial chitinase SmChiB	811:836	bacterial chitinase SmChiB	811:836	Among them, compound 6t showed the most potent activity against bacterial chitinase SmChiB and insect chitinase OfChi-h, with a Ki value of 0.14 and 0.0056 μM, respectively.
33151676	5	17	theme	chitinase	849:857	arg1	OfChi-h					859:865	insect chitinase OfChi-h	842:865	insect chitinase OfChi-h	842:865	Among them, compound 6t showed the most potent activity against bacterial chitinase SmChiB and insect chitinase OfChi-h, with a Ki value of 0.14 and 0.0056 μM, respectively.
33151676	3	18	theme	novel	446:450	arg1	chemotype					452:460	a novel chemotype	444:460	a novel chemotype of chitinase inhibitors	444:484	Here, we present a strategy of developing a novel chemotype of chitinase inhibitors by the construction of planar heterocycles that can stack with conserved aromatic residues.
33151676	5	19	theme	insect	842:847	arg1	OfChi-h					859:865	insect chitinase OfChi-h	842:865	insect chitinase OfChi-h	842:865	Among them, compound 6t showed the most potent activity against bacterial chitinase SmChiB and insect chitinase OfChi-h, with a Ki value of 0.14 and 0.0056 μM, respectively.
33151676	4	20	theme	dipyridopyrimidine-3-carboxamide	677:708	arg1	derivatives					710:720	dipyridopyrimidine-3-carboxamide derivatives	677:720	dipyridopyrimidine-3-carboxamide derivatives as chitinase inhibitors	677:744	The rational design, guided by crystallographic analysis and docking results, leads to a series of dipyridopyrimidine-3-carboxamide derivatives as chitinase inhibitors.
33151676	0	21	theme	X-ray	0:4	arg1	Structure					6:14	X-ray Structure	0:14	X-ray Structure	0:14	X-ray Structure and Molecular Docking Guided Discovery of Novel Chitinase Inhibitors with a Scaffold of Dipyridopyrimidine-3-carboxamide.
33151676	1	22	theme	chitin	210:215	arg1	degradation					195:205	the degradation	191:205	the degradation of chitin	191:215	Chitinases are the glycosyl hydrolase for catalyzing the degradation of chitin and play an indispensable role in bacterial pathogenesis, fungal cell wall remodeling, and insect molting.
33151676	0	23	theme	Molecular	20:28	arg1	Discovery					45:53	Molecular Docking Guided Discovery	20:53	Molecular Docking Guided Discovery	20:53	X-ray Structure and Molecular Docking Guided Discovery of Novel Chitinase Inhibitors with a Scaffold of Dipyridopyrimidine-3-carboxamide.
33151676	3	24	theme	chitinase	465:473	arg1	inhibitors					475:484	chitinase inhibitors	465:484	chitinase inhibitors	465:484	Here, we present a strategy of developing a novel chemotype of chitinase inhibitors by the construction of planar heterocycles that can stack with conserved aromatic residues.
33151676	4	25	theme	rational	582:589	arg1	design					591:596	The rational design	578:596	The rational design	578:596	The rational design, guided by crystallographic analysis and docking results, leads to a series of dipyridopyrimidine-3-carboxamide derivatives as chitinase inhibitors.
33151676	7	26	theme	novel	1094:1098	arg1	insights					1100:1107	novel insights	1094:1107	novel insights into developing potent chitinase inhibitors for pathogen and pest control	1094:1181	Our results provide novel insights into developing potent chitinase inhibitors for pathogen and pest control.
33151676	4	27	theme	chitinase	725:733	arg1	inhibitors					735:744	chitinase inhibitors	725:744	chitinase inhibitors	725:744	The rational design, guided by crystallographic analysis and docking results, leads to a series of dipyridopyrimidine-3-carboxamide derivatives as chitinase inhibitors.
33151676	3	28	theme	inhibitors	475:484	arg1	chemotype					452:460	a novel chemotype	444:460	a novel chemotype of chitinase inhibitors	444:484	Here, we present a strategy of developing a novel chemotype of chitinase inhibitors by the construction of planar heterocycles that can stack with conserved aromatic residues.
33151676	0	29	theme	Guided	38:43	arg1	Discovery					45:53	Molecular Docking Guided Discovery	20:53	Molecular Docking Guided Discovery	20:53	X-ray Structure and Molecular Docking Guided Discovery of Novel Chitinase Inhibitors with a Scaffold of Dipyridopyrimidine-3-carboxamide.
33151676	1	30	theme	insect	308:313	arg1	molting					315:321	insect molting	308:321	insect molting	308:321	Chitinases are the glycosyl hydrolase for catalyzing the degradation of chitin and play an indispensable role in bacterial pathogenesis, fungal cell wall remodeling, and insect molting.
33151676	5	31	theme	Ki	875:876	arg1	value					878:882	a Ki value	873:882	a Ki value of 0.14 and 0.0056 μM	873:904	Among them, compound 6t showed the most potent activity against bacterial chitinase SmChiB and insect chitinase OfChi-h, with a Ki value of 0.14 and 0.0056 μM, respectively.
33151676	0	32	theme	Docking	30:36	arg1	Discovery					45:53	Molecular Docking Guided Discovery	20:53	Molecular Docking Guided Discovery	20:53	X-ray Structure and Molecular Docking Guided Discovery of Novel Chitinase Inhibitors with a Scaffold of Dipyridopyrimidine-3-carboxamide.
33151676	5	33	theme	chitinase	821:829	arg1	SmChiB					831:836	bacterial chitinase SmChiB	811:836	bacterial chitinase SmChiB	811:836	Among them, compound 6t showed the most potent activity against bacterial chitinase SmChiB and insect chitinase OfChi-h, with a Ki value of 0.14 and 0.0056 μM, respectively.
33151676	6	34	theme	SmChiB-6p	1003:1011	arg1	structure					1024:1032	the SmChiB-6p co-crystal structure	999:1032	the SmChiB-6p co-crystal structure	999:1032	The strong stacking interaction of compound 6p with Trp99 and Trp220 found in the SmChiB-6p co-crystal structure verifies the feasibility of our design.
33151676	4	35	theme	docking	639:645	arg1	results					647:653	docking results	639:653	docking results	639:653	The rational design, guided by crystallographic analysis and docking results, leads to a series of dipyridopyrimidine-3-carboxamide derivatives as chitinase inhibitors.
33151676	1	36	theme	indispensable	229:241	arg1	role					243:246	an indispensable role	226:246	an indispensable role	226:246	Chitinases are the glycosyl hydrolase for catalyzing the degradation of chitin and play an indispensable role in bacterial pathogenesis, fungal cell wall remodeling, and insect molting.
33151676	5	37	theme	μM	903:904	arg1	value					878:882	a Ki value	873:882	a Ki value of 0.14 and 0.0056 μM	873:904	Among them, compound 6t showed the most potent activity against bacterial chitinase SmChiB and insect chitinase OfChi-h, with a Ki value of 0.14 and 0.0056 μM, respectively.
33151676	5	38	theme	potent	787:792	arg1	activity					794:801	the most potent activity	778:801	the most potent activity against bacterial chitinase SmChiB and insect chitinase OfChi-h	778:865	Among them, compound 6t showed the most potent activity against bacterial chitinase SmChiB and insect chitinase OfChi-h, with a Ki value of 0.14 and 0.0056 μM, respectively.
33151676	7	39	theme	pest	1170:1173	arg1	control					1175:1181	pest control	1170:1181	pest control	1170:1181	Our results provide novel insights into developing potent chitinase inhibitors for pathogen and pest control.
33151676	6	40	theme	compound	956:963	arg1	interaction					941:951	The strong stacking interaction	921:951	The strong stacking interaction of compound 6p with Trp99 and Trp220 found in the SmChiB-6p co-crystal structure	921:1032	The strong stacking interaction of compound 6p with Trp99 and Trp220 found in the SmChiB-6p co-crystal structure verifies the feasibility of our design.
33151676	7	41	theme	potent	1125:1130	arg1	inhibitors					1142:1151	potent chitinase inhibitors	1125:1151	potent chitinase inhibitors for pathogen and pest control	1125:1181	Our results provide novel insights into developing potent chitinase inhibitors for pathogen and pest control.
33151676	0	42	with	Structure	6:14	arg1	Scaffold					92:99	a Scaffold	90:99	a Scaffold of Dipyridopyrimidine-3-carboxamide	90:135	X-ray Structure and Molecular Docking Guided Discovery of Novel Chitinase Inhibitors with a Scaffold of Dipyridopyrimidine-3-carboxamide.
33151676	2	43	theme	attractive	345:354	arg1	chitinases					330:339	chitinases	330:339	chitinases	330:339	Thus, chitinases are attractive targets for therapeutic drugs and pesticides.
33151676	2	43	theme	attractive	345:354	arg1	targets					356:362	attractive targets	345:362	attractive targets for therapeutic drugs and pesticides	345:399	Thus, chitinases are attractive targets for therapeutic drugs and pesticides.
33151676	6	44	with	interaction	941:951	arg1	Trp220					983:988	Trp220	983:988	Trp220	983:988	The strong stacking interaction of compound 6p with Trp99 and Trp220 found in the SmChiB-6p co-crystal structure verifies the feasibility of our design.
33151676	6	44	with	interaction	941:951	arg1	Trp99					973:977	Trp99	973:977	Trp99	973:977	The strong stacking interaction of compound 6p with Trp99 and Trp220 found in the SmChiB-6p co-crystal structure verifies the feasibility of our design.
33151676	6	45	located	found	990:994	arg2	Trp99					973:977	Trp99	973:977	Trp99	973:977	The strong stacking interaction of compound 6p with Trp99 and Trp220 found in the SmChiB-6p co-crystal structure verifies the feasibility of our design.
33151676	6	45	located	found	990:994	arg1	structure					1024:1032	the SmChiB-6p co-crystal structure	999:1032	the SmChiB-6p co-crystal structure	999:1032	The strong stacking interaction of compound 6p with Trp99 and Trp220 found in the SmChiB-6p co-crystal structure verifies the feasibility of our design.
33151676	4	46	theme	crystallographic	609:624	arg1	analysis					626:633	crystallographic analysis	609:633	crystallographic analysis	609:633	The rational design, guided by crystallographic analysis and docking results, leads to a series of dipyridopyrimidine-3-carboxamide derivatives as chitinase inhibitors.
33151676	0	47	theme	Chitinase	64:72	arg1	Inhibitors					74:83	Novel Chitinase Inhibitors	58:83	Novel Chitinase Inhibitors	58:83	X-ray Structure and Molecular Docking Guided Discovery of Novel Chitinase Inhibitors with a Scaffold of Dipyridopyrimidine-3-carboxamide.
33151676	0	48	with	Discovery	45:53	arg1	Scaffold					92:99	a Scaffold	90:99	a Scaffold of Dipyridopyrimidine-3-carboxamide	90:135	X-ray Structure and Molecular Docking Guided Discovery of Novel Chitinase Inhibitors with a Scaffold of Dipyridopyrimidine-3-carboxamide.
33151676	1	49	theme	glycosyl	157:164	arg1	hydrolase					166:174	the glycosyl hydrolase	153:174	the glycosyl hydrolase	153:174	Chitinases are the glycosyl hydrolase for catalyzing the degradation of chitin and play an indispensable role in bacterial pathogenesis, fungal cell wall remodeling, and insect molting.
33151676	1	49	theme	glycosyl	157:164	arg1	Chitinases					138:147	Chitinases	138:147	Chitinases	138:147	Chitinases are the glycosyl hydrolase for catalyzing the degradation of chitin and play an indispensable role in bacterial pathogenesis, fungal cell wall remodeling, and insect molting.
33151676	1	50	theme	bacterial	251:259	arg1	pathogenesis					261:272	bacterial pathogenesis	251:272	bacterial pathogenesis	251:272	Chitinases are the glycosyl hydrolase for catalyzing the degradation of chitin and play an indispensable role in bacterial pathogenesis, fungal cell wall remodeling, and insect molting.
33151676	0	51	theme	Novel	58:62	arg1	Inhibitors					74:83	Novel Chitinase Inhibitors	58:83	Novel Chitinase Inhibitors	58:83	X-ray Structure and Molecular Docking Guided Discovery of Novel Chitinase Inhibitors with a Scaffold of Dipyridopyrimidine-3-carboxamide.
33151676	4	52	theme	derivatives	710:720	arg1	series					667:672	a series	665:672	a series of dipyridopyrimidine-3-carboxamide derivatives as chitinase inhibitors	665:744	The rational design, guided by crystallographic analysis and docking results, leads to a series of dipyridopyrimidine-3-carboxamide derivatives as chitinase inhibitors.
34885872	0	0	theme	Fireweed	91:98	arg1	Polysaccharides					72:86	Pectic Polysaccharides	65:86	Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.)	65:127	Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.).
34885872	2	1	from	radical	532:538	arg1	manner					569:574	a concentration-dependent manner	543:574	a concentration-dependent manner	543:574	EA-4.0 and EA-0.8 were found to scavenge the DPPH radical in a concentration-dependent manner at 17-133 μg/mL, whereas commercial apple pectin scavenged at 0.5-2 mg/mL.
34885872	1	2	attach	isolated	330:337	arg2	pectins					251:257	Two pectins	247:257	Two pectins	247:257	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	1	2	attach	isolated	330:337	arg2	EA-0.8					271:276	EA-0.8	271:276	EA-0.8	271:276	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	1	2	attach	isolated	330:337	arg1	the					344:346	the	344:346	the	344:346	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	1	2	attach	isolated	330:337	arg2	EA-4.0					260:265	EA-4.0	260:265	EA-4.0	260:265	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	5	3	theme	Correlation	980:990	arg1	analysis					992:999	Correlation analysis	980:999	Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography	980:1128	Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography revealed that the antioxidant capacity of fireweed pectins is mainly due to phenolics and is partially associated with xylogalacturonan chains.
34885872	3	4	theme	apple	755:759	arg1	pectin					761:766	a commercial apple pectin	742:766	a commercial apple pectin	742:766	The antioxidant activity of EA-4.0 was the highest and exceeded the activity of EA-0.8 and a commercial apple pectin by 2 and 39 times (IC50-0.050, 0.109 and 1.961 mg/mL), respectively.
34885872	1	5	theme	88	314:315	arg1	contents					302:309	galacturonic acid contents	284:309	galacturonic acid contents of 88 and 91%	284:323	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	2	6	theme	DPPH	527:530	arg1	radical					532:538	the DPPH radical	523:538	the DPPH radical in a concentration-dependent manner at 17-133 μg/mL	523:590	EA-4.0 and EA-0.8 were found to scavenge the DPPH radical in a concentration-dependent manner at 17-133 μg/mL, whereas commercial apple pectin scavenged at 0.5-2 mg/mL.
34885872	6	7	with	fireweed	1371:1378	arg1	activity					1409:1416	high antioxidant activity	1392:1416	high antioxidant activity	1392:1416	The data obtained demonstrate that pectic polysaccharides appeared to be bioactive components of fireweed leaves with high antioxidant activity, which depend on pH at their extraction.
34885872	1	8	theme	study	146:150	arg1	aim					134:136	The aim	130:136	The aim of this study	130:150	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	5	9	theme	pectins	1181:1187	arg1	due					1199:1201	due	1199:1201	due	1199:1201	Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography revealed that the antioxidant capacity of fireweed pectins is mainly due to phenolics and is partially associated with xylogalacturonan chains.
34885872	5	9	theme	pectins	1181:1187	arg1	capacity					1160:1167	the antioxidant capacity	1144:1167	the antioxidant capacity of fireweed pectins	1144:1187	Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography revealed that the antioxidant capacity of fireweed pectins is mainly due to phenolics and is partially associated with xylogalacturonan chains.
34885872	3	10	theme	pectin	761:766	arg1	activity					719:726	the activity	715:726	the activity of EA-0.8 and a commercial apple pectin	715:766	The antioxidant activity of EA-4.0 was the highest and exceeded the activity of EA-0.8 and a commercial apple pectin by 2 and 39 times (IC50-0.050, 0.109 and 1.961 mg/mL), respectively.
34885872	0	11	theme	Epilobium	101:109	arg1	Fireweed					91:98	Fireweed	91:98	Fireweed (Epilobium angustifolium L.)	91:127	Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.).
34885872	0	11	theme	Epilobium	101:109	arg1	L					125:125	Epilobium angustifolium L.	101:126	Epilobium angustifolium L.	101:126	Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.).
34885872	2	12	theme	apple	612:616	arg1	pectin					618:623	commercial apple pectin	601:623	commercial apple pectin	601:623	EA-4.0 and EA-0.8 were found to scavenge the DPPH radical in a concentration-dependent manner at 17-133 μg/mL, whereas commercial apple pectin scavenged at 0.5-2 mg/mL.
34885872	5	13	theme	fireweed	1172:1179	arg1	pectins					1181:1187	fireweed pectins	1172:1187	fireweed pectins	1172:1187	Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography revealed that the antioxidant capacity of fireweed pectins is mainly due to phenolics and is partially associated with xylogalacturonan chains.
34885872	6	14	theme	high	1392:1395	arg1	activity					1409:1416	high antioxidant activity	1392:1416	high antioxidant activity	1392:1416	The data obtained demonstrate that pectic polysaccharides appeared to be bioactive components of fireweed leaves with high antioxidant activity, which depend on pH at their extraction.
34885872	1	15	dep	E.	358:359	arg1	L.					375:376	E. angustifolium L.	358:376	E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively	358:479	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	3	16	theme	antioxidant	655:665	arg1	highest					694:700	highest	694:700	highest	694:700	The antioxidant activity of EA-4.0 was the highest and exceeded the activity of EA-0.8 and a commercial apple pectin by 2 and 39 times (IC50-0.050, 0.109 and 1.961 mg/mL), respectively.
34885872	3	16	theme	antioxidant	655:665	arg1	activity					667:674	The antioxidant activity	651:674	The antioxidant activity of EA-4.0	651:684	The antioxidant activity of EA-4.0 was the highest and exceeded the activity of EA-0.8 and a commercial apple pectin by 2 and 39 times (IC50-0.050, 0.109 and 1.961 mg/mL), respectively.
34885872	2	17	theme	0.5-2	638:642	arg1	mg/mL					644:648	0.5-2 mg/mL	638:648	0.5-2 mg/mL	638:648	EA-4.0 and EA-0.8 were found to scavenge the DPPH radical in a concentration-dependent manner at 17-133 μg/mL, whereas commercial apple pectin scavenged at 0.5-2 mg/mL.
34885872	3	18	theme	1.961	809:813	arg1	mg/mL					815:819	IC50-0.050, 0.109 and 1.961 mg/mL	787:819	mg/mL	815:819	The antioxidant activity of EA-4.0 was the highest and exceeded the activity of EA-0.8 and a commercial apple pectin by 2 and 39 times (IC50-0.050, 0.109 and 1.961 mg/mL), respectively.
34885872	3	18	theme	1.961	809:813	arg1	times					780:784	2 and 39 times	771:784	2 and 39 times (IC50-0.050, 0.109 and 1.961 mg/mL)	771:820	The antioxidant activity of EA-4.0 was the highest and exceeded the activity of EA-0.8 and a commercial apple pectin by 2 and 39 times (IC50-0.050, 0.109 and 1.961 mg/mL), respectively.
34885872	3	19	theme	commercial	744:753	arg1	pectin					761:766	a commercial apple pectin	742:766	a commercial apple pectin	742:766	The antioxidant activity of EA-4.0 was the highest and exceeded the activity of EA-0.8 and a commercial apple pectin by 2 and 39 times (IC50-0.050, 0.109 and 1.961 mg/mL), respectively.
34885872	1	20	theme	%	323:323	arg1	contents					302:309	galacturonic acid contents	284:309	galacturonic acid contents of 88 and 91%	284:323	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	1	21	theme	antioxidant	180:190	arg1	activity					192:199	antioxidant activity	180:199	antioxidant activity	180:199	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	5	22	theme	xylogalacturonan	1249:1264	arg1	chains					1266:1271	xylogalacturonan chains	1249:1271	xylogalacturonan chains	1249:1271	Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography revealed that the antioxidant capacity of fireweed pectins is mainly due to phenolics and is partially associated with xylogalacturonan chains.
34885872	6	23	theme	pectic	1309:1314	arg1	polysaccharides					1316:1330	pectic polysaccharides	1309:1330	pectic polysaccharides	1309:1330	The data obtained demonstrate that pectic polysaccharides appeared to be bioactive components of fireweed leaves with high antioxidant activity, which depend on pH at their extraction.
34885872	0	24	theme	angustifolium	111:123	arg1	Fireweed					91:98	Fireweed	91:98	Fireweed (Epilobium angustifolium L.)	91:127	Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.).
34885872	0	24	theme	angustifolium	111:123	arg1	L					125:125	Epilobium angustifolium L.	101:126	Epilobium angustifolium L.	101:126	Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.).
34885872	2	25	theme	concentration-dependent	545:567	arg1	manner					569:574	a concentration-dependent manner	543:574	a concentration-dependent manner	543:574	EA-4.0 and EA-0.8 were found to scavenge the DPPH radical in a concentration-dependent manner at 17-133 μg/mL, whereas commercial apple pectin scavenged at 0.5-2 mg/mL.
34885872	1	26	theme	raw	404:406	arg1	materials					408:416	plant raw materials	398:416	plant raw materials	398:416	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	5	27	theme	fractions	1054:1062	arg1	activity					1024:1031	activity	1024:1031	activity	1024:1031	Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography revealed that the antioxidant capacity of fireweed pectins is mainly due to phenolics and is partially associated with xylogalacturonan chains.
34885872	5	27	theme	fractions	1054:1062	arg1	composition					1008:1018	composition	1008:1018	composition	1008:1018	Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography revealed that the antioxidant capacity of fireweed pectins is mainly due to phenolics and is partially associated with xylogalacturonan chains.
34885872	1	28	theme	materials	408:416	arg1	treatment					385:393	the treatment	381:393	the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively	381:479	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	6	29	theme	antioxidant	1397:1407	arg1	activity					1409:1416	high antioxidant activity	1392:1416	high antioxidant activity	1392:1416	The data obtained demonstrate that pectic polysaccharides appeared to be bioactive components of fireweed leaves with high antioxidant activity, which depend on pH at their extraction.
34885872	5	30	theme	activity	1024:1031	arg1	analysis					992:999	Correlation analysis	980:999	Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography	980:1128	Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography revealed that the antioxidant capacity of fireweed pectins is mainly due to phenolics and is partially associated with xylogalacturonan chains.
34885872	5	31	theme	anionic	1098:1104	arg1	chromatography					1115:1128	anionic exchange chromatography	1098:1128	anionic exchange chromatography	1098:1128	Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography revealed that the antioxidant capacity of fireweed pectins is mainly due to phenolics and is partially associated with xylogalacturonan chains.
34885872	2	32	theme	commercial	601:610	arg1	pectin					618:623	commercial apple pectin	601:623	commercial apple pectin	601:623	EA-4.0 and EA-0.8 were found to scavenge the DPPH radical in a concentration-dependent manner at 17-133 μg/mL, whereas commercial apple pectin scavenged at 0.5-2 mg/mL.
34885872	4	33	theme	equal	936:940	arg1	IC50s					930:934	IC50s	930:934	IC50s equal to 0.27 and 0.97 mg/mL, respectively	930:977	Pectins EA-4.0 and EA-0.8 were found to possess superoxide radical scavenging activity, with IC50s equal to 0.27 and 0.97 mg/mL, respectively.
34885872	5	34	theme	enzyme	1076:1081	arg1	hydrolysis					1083:1092	enzyme hydrolysis	1076:1092	enzyme hydrolysis	1076:1092	Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography revealed that the antioxidant capacity of fireweed pectins is mainly due to phenolics and is partially associated with xylogalacturonan chains.
34885872	1	35	theme	aqueous	423:429	arg1	acid					444:447	aqueous hydrochloric acid	423:447	aqueous hydrochloric acid at pH 4.0 and 0.8, respectively	423:479	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	0	36	theme	Chemical	11:18	arg1	Characterization					20:35	Chemical Characterization	11:35	Chemical Characterization	11:35	Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.).
34885872	1	37	theme	plant	398:402	arg1	materials					408:416	plant raw materials	398:416	plant raw materials	398:416	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	6	38	theme	bioactive	1347:1355	arg1	components					1357:1366	bioactive components	1347:1366	bioactive components of fireweed leaves with high antioxidant activity	1347:1416	The data obtained demonstrate that pectic polysaccharides appeared to be bioactive components of fireweed leaves with high antioxidant activity, which depend on pH at their extraction.
34885872	6	39	from	extraction	1447:1456	arg1	pH					1435:1436	pH	1435:1436	pH at their extraction	1435:1456	The data obtained demonstrate that pectic polysaccharides appeared to be bioactive components of fireweed leaves with high antioxidant activity, which depend on pH at their extraction.
34885872	5	40	dep	composition	1008:1018	arg1	the					1004:1006	the	1004:1006	the	1004:1006	Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography revealed that the antioxidant capacity of fireweed pectins is mainly due to phenolics and is partially associated with xylogalacturonan chains.
34885872	1	41	from	pH	452:453	arg1	acid					444:447	aqueous hydrochloric acid	423:447	aqueous hydrochloric acid at pH 4.0 and 0.8, respectively	423:479	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	0	42	theme	Antioxidant	41:51	arg1	Activity					53:60	Antioxidant Activity	41:60	Antioxidant Activity	41:60	Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.).
34885872	3	43	theme	EA-4.0	679:684	arg1	highest					694:700	highest	694:700	highest	694:700	The antioxidant activity of EA-4.0 was the highest and exceeded the activity of EA-0.8 and a commercial apple pectin by 2 and 39 times (IC50-0.050, 0.109 and 1.961 mg/mL), respectively.
34885872	3	43	theme	EA-4.0	679:684	arg1	activity					667:674	The antioxidant activity	651:674	The antioxidant activity of EA-4.0	651:684	The antioxidant activity of EA-4.0 was the highest and exceeded the activity of EA-0.8 and a commercial apple pectin by 2 and 39 times (IC50-0.050, 0.109 and 1.961 mg/mL), respectively.
34885872	1	44	dep	Epilobium	220:228	arg1	L.					244:245	Epilobium angustifolium L.	220:245	Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively	220:479	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	1	44	dep	Epilobium	220:228	arg1	isolated					330:337	isolated	330:337	were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively	325:479	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	4	45	theme	superoxide	885:894	arg1	activity					915:922	superoxide radical scavenging activity	885:922	superoxide radical scavenging activity	885:922	Pectins EA-4.0 and EA-0.8 were found to possess superoxide radical scavenging activity, with IC50s equal to 0.27 and 0.97 mg/mL, respectively.
34885872	5	46	theme	exchange	1106:1113	arg1	chromatography					1115:1128	anionic exchange chromatography	1098:1128	anionic exchange chromatography	1098:1128	Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography revealed that the antioxidant capacity of fireweed pectins is mainly due to phenolics and is partially associated with xylogalacturonan chains.
34885872	1	47	dep	the	206:208	arg1	leaves					210:215	leaves	210:215	leaves	210:215	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	6	48	theme	fireweed	1371:1378	arg1	components					1357:1366	bioactive components	1347:1366	bioactive components of fireweed leaves with high antioxidant activity	1347:1416	The data obtained demonstrate that pectic polysaccharides appeared to be bioactive components of fireweed leaves with high antioxidant activity, which depend on pH at their extraction.
34885872	1	49	theme	hydrochloric	431:442	arg1	acid					444:447	aqueous hydrochloric acid	423:447	aqueous hydrochloric acid at pH 4.0 and 0.8, respectively	423:479	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	1	50	theme	acid	297:300	arg1	contents					302:309	galacturonic acid contents	284:309	galacturonic acid contents of 88 and 91%	284:323	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	5	51	theme	polysaccharide	1039:1052	arg1	fractions					1054:1062	32 polysaccharide fractions	1036:1062	32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography	1036:1128	Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography revealed that the antioxidant capacity of fireweed pectins is mainly due to phenolics and is partially associated with xylogalacturonan chains.
34885872	6	52	dep	fireweed	1371:1378	arg1	leaves					1380:1385	leaves	1380:1385	leaves	1380:1385	The data obtained demonstrate that pectic polysaccharides appeared to be bioactive components of fireweed leaves with high antioxidant activity, which depend on pH at their extraction.
34885872	1	53	with	pectins	251:257	arg1	contents					302:309	galacturonic acid contents	284:309	galacturonic acid contents of 88 and 91%	284:323	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	4	54	theme	scavenging	904:913	arg1	activity					915:922	superoxide radical scavenging activity	885:922	superoxide radical scavenging activity	885:922	Pectins EA-4.0 and EA-0.8 were found to possess superoxide radical scavenging activity, with IC50s equal to 0.27 and 0.97 mg/mL, respectively.
34885872	1	55	theme	galacturonic	284:295	arg1	contents					302:309	galacturonic acid contents	284:309	galacturonic acid contents of 88 and 91%	284:323	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	1	56	with	pectins	167:173	arg1	activity					192:199	antioxidant activity	180:199	antioxidant activity	180:199	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	1	57	with	treatment	385:393	arg1	acid					444:447	aqueous hydrochloric acid	423:447	aqueous hydrochloric acid at pH 4.0 and 0.8, respectively	423:479	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34885872	3	58	theme	EA-0.8	731:736	arg1	activity					719:726	the activity	715:726	the activity of EA-0.8 and a commercial apple pectin	715:766	The antioxidant activity of EA-4.0 was the highest and exceeded the activity of EA-0.8 and a commercial apple pectin by 2 and 39 times (IC50-0.050, 0.109 and 1.961 mg/mL), respectively.
34885872	4	59	theme	radical	896:902	arg1	activity					915:922	superoxide radical scavenging activity	885:922	superoxide radical scavenging activity	885:922	Pectins EA-4.0 and EA-0.8 were found to possess superoxide radical scavenging activity, with IC50s equal to 0.27 and 0.97 mg/mL, respectively.
34885872	2	60	from	μg/mL	586:590	arg1	radical					532:538	the DPPH radical	523:538	the DPPH radical in a concentration-dependent manner at 17-133 μg/mL	523:590	EA-4.0 and EA-0.8 were found to scavenge the DPPH radical in a concentration-dependent manner at 17-133 μg/mL, whereas commercial apple pectin scavenged at 0.5-2 mg/mL.
34885872	0	61	theme	Polysaccharides	72:86	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.).
34885872	0	61	theme	Polysaccharides	72:86	arg1	Characterization					20:35	Chemical Characterization	11:35	Chemical Characterization	11:35	Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.).
34885872	0	61	theme	Polysaccharides	72:86	arg1	Activity					53:60	Antioxidant Activity	41:60	Antioxidant Activity	41:60	Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.).
34885872	4	62	contain	possess	877:883	arg1	Pectins					837:843	Pectins EA-4.0 and EA-0.8	837:861	Pectins	837:843	Pectins EA-4.0 and EA-0.8 were found to possess superoxide radical scavenging activity, with IC50s equal to 0.27 and 0.97 mg/mL, respectively.
34885872	4	62	contain	possess	877:883	arg1	EA-0.8					856:861	EA-0.8	856:861	EA-0.8	856:861	Pectins EA-4.0 and EA-0.8 were found to possess superoxide radical scavenging activity, with IC50s equal to 0.27 and 0.97 mg/mL, respectively.
34885872	4	62	contain	possess	877:883	arg2	activity					915:922	superoxide radical scavenging activity	885:922	superoxide radical scavenging activity	885:922	Pectins EA-4.0 and EA-0.8 were found to possess superoxide radical scavenging activity, with IC50s equal to 0.27 and 0.97 mg/mL, respectively.
34885872	5	63	theme	antioxidant	1148:1158	arg1	due					1199:1201	due	1199:1201	due	1199:1201	Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography revealed that the antioxidant capacity of fireweed pectins is mainly due to phenolics and is partially associated with xylogalacturonan chains.
34885872	5	63	theme	antioxidant	1148:1158	arg1	capacity					1160:1167	the antioxidant capacity	1144:1167	the antioxidant capacity of fireweed pectins	1144:1187	Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography revealed that the antioxidant capacity of fireweed pectins is mainly due to phenolics and is partially associated with xylogalacturonan chains.
34885872	0	64	theme	Pectic	65:70	arg1	Polysaccharides					72:86	Pectic Polysaccharides	65:86	Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.)	65:127	Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.).
34885872	5	65	theme	composition	1008:1018	arg1	analysis					992:999	Correlation analysis	980:999	Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography	980:1128	Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography revealed that the antioxidant capacity of fireweed pectins is mainly due to phenolics and is partially associated with xylogalacturonan chains.
34885872	3	66	theme	IC50-0.050	787:796	arg1	mg/mL					815:819	IC50-0.050, 0.109 and 1.961 mg/mL	787:819	mg/mL	815:819	The antioxidant activity of EA-4.0 was the highest and exceeded the activity of EA-0.8 and a commercial apple pectin by 2 and 39 times (IC50-0.050, 0.109 and 1.961 mg/mL), respectively.
34885872	3	66	theme	IC50-0.050	787:796	arg1	times					780:784	2 and 39 times	771:784	2 and 39 times (IC50-0.050, 0.109 and 1.961 mg/mL)	771:820	The antioxidant activity of EA-4.0 was the highest and exceeded the activity of EA-0.8 and a commercial apple pectin by 2 and 39 times (IC50-0.050, 0.109 and 1.961 mg/mL), respectively.
34885872	1	67	dep	the	344:346	arg1	leaves					348:353	leaves	348:353	leaves	348:353	The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively.
34925283	8	0	theme	various	1366:1372	arg1	facets					1374:1379	various facets	1366:1379	various facets of Hbt	1366:1386	Moreover, this study demonstrated how compromised N-glycosylation affects various facets of Hbt.
34925283	3	1	from	impact	579:584	arg1	N-glycosylation					589:603	N-glycosylation	589:603	N-glycosylation	589:603	Following deletion of each encoding gene, the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism, was assessed by mass spectrometry.
34925283	3	1	from	impact	579:584	arg1	organism					678:685	this organism	673:685	this organism	673:685	Following deletion of each encoding gene, the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism, was assessed by mass spectrometry.
34925283	9	2	theme	archaellin-encoding	1445:1463	arg1	genes					1465:1469	archaellin-encoding genes	1445:1469	archaellin-encoding genes	1445:1469	salinarum cell behavior, including the transcription of archaellin-encoding genes.
34925283	5	3	theme	biochemical	930:940	arg1	assays					972:977	biochemical, structural and physiological assays	930:977	biochemical, structural and physiological assays	930:977	Finally, the impacts of such deletions were characterized in a series of biochemical, structural and physiological assays.
34925283	3	4	theme	mass	704:707	arg1	spectrometry					709:720	mass spectrometry	704:720	mass spectrometry	704:720	Following deletion of each encoding gene, the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism, was assessed by mass spectrometry.
34925283	3	5	theme	encoding	560:567	arg1	gene					569:572	each encoding gene	555:572	each encoding gene	555:572	Following deletion of each encoding gene, the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism, was assessed by mass spectrometry.
34925283	9	6	theme	genes	1465:1469	arg1	transcription					1428:1440	the transcription	1424:1440	the transcription of archaellin-encoding genes	1424:1469	salinarum cell behavior, including the transcription of archaellin-encoding genes.
34925283	2	7	from	glycoproteins	488:500	arg1	species					510:516	this species	505:516	this species	505:516	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	6	8	theme	tetrasaccharide	1235:1249	arg1	sugars					1212:1217	the second, third and fourth sugars	1183:1217	the second, third and fourth sugars of the N-linked tetrasaccharide decorating Hbt	1183:1264	The results revealed that VNG1067G, VNG1066C, and VNG1062G, renamed Agl25, Agl26, and Agl27 according to the nomenclature used for archaeal N-glycosylation pathway components, are responsible for adding the second, third and fourth sugars of the N-linked tetrasaccharide decorating Hbt.
34925283	6	9	theme	second	1187:1192	arg1	sugars					1212:1217	the second, third and fourth sugars	1183:1217	the second, third and fourth sugars of the N-linked tetrasaccharide decorating Hbt	1183:1264	The results revealed that VNG1067G, VNG1066C, and VNG1062G, renamed Agl25, Agl26, and Agl27 according to the nomenclature used for archaeal N-glycosylation pathway components, are responsible for adding the second, third and fourth sugars of the N-linked tetrasaccharide decorating Hbt.
34925283	2	10	theme	pathways	310:317	arg1	number					264:269	the number	260:269	the number of delineated archaeal N-glycosylation pathways	260:317	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	3	11	from	glycoproteins	656:668	arg1	organism					678:685	this organism	673:685	this organism	673:685	Following deletion of each encoding gene, the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism, was assessed by mass spectrometry.
34925283	5	12	theme	structural	943:952	arg1	assays					972:977	biochemical, structural and physiological assays	930:977	biochemical, structural and physiological assays	930:977	Finally, the impacts of such deletions were characterized in a series of biochemical, structural and physiological assays.
34925283	3	13	gly	glycoprotein	620:631	arg1	glycoprotein					620:631	S-layer glycoprotein	612:631	S-layer glycoprotein	612:631	Following deletion of each encoding gene, the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism, was assessed by mass spectrometry.
34925283	2	14	theme	N-glycosylation	294:308	arg1	pathways					310:317	delineated archaeal N-glycosylation pathways	274:317	delineated archaeal N-glycosylation pathways	274:317	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	6	15	dep	renamed	1040:1046	arg1	according					1072:1080	according	1072:1080	according to the nomenclature used for archaeal N-glycosylation pathway components	1072:1153	The results revealed that VNG1067G, VNG1066C, and VNG1062G, renamed Agl25, Agl26, and Agl27 according to the nomenclature used for archaeal N-glycosylation pathway components, are responsible for adding the second, third and fourth sugars of the N-linked tetrasaccharide decorating Hbt.
34925283	8	16	theme	Hbt	1384:1386	arg1	facets					1374:1379	various facets	1366:1379	various facets of Hbt	1366:1386	Moreover, this study demonstrated how compromised N-glycosylation affects various facets of Hbt.
34925283	2	17	theme	archaeal	285:292	arg1	pathways					310:317	delineated archaeal N-glycosylation pathways	274:317	delineated archaeal N-glycosylation pathways	274:317	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	5	18	theme	such	881:884	arg1	deletions					886:894	such deletions	881:894	such deletions	881:894	Finally, the impacts of such deletions were characterized in a series of biochemical, structural and physiological assays.
34925283	6	19	link	N-linked	1226:1233	arg1	tetrasaccharide					1235:1249	the N-linked tetrasaccharide	1222:1249	the N-linked tetrasaccharide decorating Hbt	1222:1264	The results revealed that VNG1067G, VNG1066C, and VNG1062G, renamed Agl25, Agl26, and Agl27 according to the nomenclature used for archaeal N-glycosylation pathway components, are responsible for adding the second, third and fourth sugars of the N-linked tetrasaccharide decorating Hbt.
34925283	7	20	theme	salinarum	1267:1275	arg1	glycoproteins					1277:1289	salinarum glycoproteins	1267:1289	salinarum glycoproteins	1267:1289	salinarum glycoproteins.
34925283	3	21	theme	S-layer	612:618	arg1	glycoprotein					620:631	S-layer glycoprotein	612:631	S-layer glycoprotein	612:631	Following deletion of each encoding gene, the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism, was assessed by mass spectrometry.
34925283	3	22	from	organism	678:685	arg1	impact					579:584	the impact	575:584	the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism,	575:686	Following deletion of each encoding gene, the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism, was assessed by mass spectrometry.
34925283	5	23	theme	deletions	886:894	arg1	impacts					870:876	the impacts	866:876	the impacts of such deletions	866:894	Finally, the impacts of such deletions were characterized in a series of biochemical, structural and physiological assays.
34925283	3	24	theme	glycoprotein	620:631	arg1	impact					579:584	the impact	575:584	the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism,	575:686	Following deletion of each encoding gene, the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism, was assessed by mass spectrometry.
34925283	3	25	gly	glycoproteins	656:668	arg1	glycoproteins					656:668	major glycoproteins	650:668	major glycoproteins in this organism	650:685	Following deletion of each encoding gene, the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism, was assessed by mass spectrometry.
34925283	1	26	theme	universal	116:124	arg1	N-glycosylation					85:99	N-glycosylation	85:99	N-glycosylation	85:99	Whereas N-glycosylation is a seemingly universal process in Archaea, pathways of N-glycosylation have only been experimentally verified in a mere handful of species.
34925283	1	26	theme	universal	116:124	arg1	process					126:132	a seemingly universal process	104:132	a seemingly universal process in Archaea	104:143	Whereas N-glycosylation is a seemingly universal process in Archaea, pathways of N-glycosylation have only been experimentally verified in a mere handful of species.
34925283	3	27	from	archaellins	637:647	arg1	organism					678:685	this organism	673:685	this organism	673:685	Following deletion of each encoding gene, the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism, was assessed by mass spectrometry.
34925283	2	28	theme	N-linked	452:459	arg1	tetrasaccharide					461:475	an N-linked tetrasaccharide	449:475	an N-linked tetrasaccharide decorating glycoproteins in this species	449:516	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	2	29	theme	tetrasaccharide	461:475	arg1	assembly					437:444	the assembly	433:444	the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species	433:516	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	5	30	theme	physiological	958:970	arg1	assays					972:977	biochemical, structural and physiological assays	930:977	biochemical, structural and physiological assays	930:977	Finally, the impacts of such deletions were characterized in a series of biochemical, structural and physiological assays.
34925283	5	31	theme	assays	972:977	arg1	series					920:925	a series	918:925	a series of biochemical, structural and physiological assays	918:977	Finally, the impacts of such deletions were characterized in a series of biochemical, structural and physiological assays.
34925283	3	32	dep	glycoprotein	620:631	arg1	the					608:610	the	608:610	the	608:610	Following deletion of each encoding gene, the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism, was assessed by mass spectrometry.
34925283	1	33	theme	mere	218:221	arg1	handful					223:229	a mere handful	216:229	a mere handful of species	216:240	Whereas N-glycosylation is a seemingly universal process in Archaea, pathways of N-glycosylation have only been experimentally verified in a mere handful of species.
34925283	0	34	theme	salinarum	42:50	arg1	Pathway					68:74	a Halobacterium salinarum N-Glycosylation Pathway	26:74	a Halobacterium salinarum N-Glycosylation Pathway	26:74	Identifying Components of a Halobacterium salinarum N-Glycosylation Pathway.
34925283	4	35	theme	dolichol	745:752	arg1	phosphate					754:762	dolichol phosphate	745:762	dolichol phosphate	745:762	Likewise, the pool of dolichol phosphate, the lipid upon which this glycan is assembled, was also considered in each deletion strain.
34925283	4	35	theme	dolichol	745:752	arg1	lipid					769:773	the lipid	765:773	the lipid upon which this glycan is assembled	765:809	Likewise, the pool of dolichol phosphate, the lipid upon which this glycan is assembled, was also considered in each deletion strain.
34925283	6	36	theme	pathway	1136:1142	arg1	components					1144:1153	archaeal N-glycosylation pathway components	1111:1153	archaeal N-glycosylation pathway components	1111:1153	The results revealed that VNG1067G, VNG1066C, and VNG1062G, renamed Agl25, Agl26, and Agl27 according to the nomenclature used for archaeal N-glycosylation pathway components, are responsible for adding the second, third and fourth sugars of the N-linked tetrasaccharide decorating Hbt.
34925283	4	37	theme	deletion	840:847	arg1	strain					849:854	each deletion strain	835:854	each deletion strain	835:854	Likewise, the pool of dolichol phosphate, the lipid upon which this glycan is assembled, was also considered in each deletion strain.
34925283	6	38	theme	third	1195:1199	arg1	sugars					1212:1217	the second, third and fourth sugars	1183:1217	the second, third and fourth sugars of the N-linked tetrasaccharide decorating Hbt	1183:1264	The results revealed that VNG1067G, VNG1066C, and VNG1062G, renamed Agl25, Agl26, and Agl27 according to the nomenclature used for archaeal N-glycosylation pathway components, are responsible for adding the second, third and fourth sugars of the N-linked tetrasaccharide decorating Hbt.
34925283	0	39	theme	Halobacterium	28:40	arg1	Pathway					68:74	a Halobacterium salinarum N-Glycosylation Pathway	26:74	a Halobacterium salinarum N-Glycosylation Pathway	26:74	Identifying Components of a Halobacterium salinarum N-Glycosylation Pathway.
34925283	9	40	theme	salinarum	1389:1397	arg1	behavior					1404:1411	salinarum cell behavior,	1389:1412	behavior	1404:1411	salinarum cell behavior, including the transcription of archaellin-encoding genes.
34925283	8	41	theme	compromised	1330:1340	arg1	N-glycosylation					1342:1356	compromised N-glycosylation	1330:1356	compromised N-glycosylation	1330:1356	Moreover, this study demonstrated how compromised N-glycosylation affects various facets of Hbt.
34925283	3	42	theme	major	650:654	arg1	glycoproteins					656:668	major glycoproteins	650:668	major glycoproteins in this organism	650:685	Following deletion of each encoding gene, the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism, was assessed by mass spectrometry.
34925283	0	43	theme	Pathway	68:74	arg1	Components					12:21	Components	12:21	Components of a Halobacterium salinarum N-Glycosylation Pathway	12:74	Identifying Components of a Halobacterium salinarum N-Glycosylation Pathway.
34925283	9	44	theme	cell	1399:1402	arg1	behavior					1404:1411	salinarum cell behavior,	1389:1412	behavior	1404:1411	salinarum cell behavior, including the transcription of archaellin-encoding genes.
34925283	2	45	theme	glycosyltransferases	376:395	arg1	involvement					324:334	the involvement	320:334	the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species	320:516	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	0	46	theme	N-Glycosylation	52:66	arg1	Pathway					68:74	a Halobacterium salinarum N-Glycosylation Pathway	26:74	a Halobacterium salinarum N-Glycosylation Pathway	26:74	Identifying Components of a Halobacterium salinarum N-Glycosylation Pathway.
34925283	3	47	theme	archaellins	637:647	arg1	impact					579:584	the impact	575:584	the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism,	575:686	Following deletion of each encoding gene, the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism, was assessed by mass spectrometry.
34925283	2	48	theme	salinarum	366:374	arg1	glycosyltransferases					376:395	the putative Halobacterium salinarum glycosyltransferases	339:395	the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G	339:428	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	2	48	theme	salinarum	366:374	arg1	VNG1066C					407:414	VNG1066C	407:414	VNG1066C	407:414	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	2	48	theme	salinarum	366:374	arg1	VNG1062G					421:428	VNG1062G	421:428	VNG1062G	421:428	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	2	48	theme	salinarum	366:374	arg1	VNG1067G					397:404	VNG1067G	397:404	VNG1067G	397:404	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	2	49	dep	glycosyltransferases	376:395	arg1	glycosyltransferases					376:395	the putative Halobacterium salinarum glycosyltransferases	339:395	the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G	339:428	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	2	49	dep	glycosyltransferases	376:395	arg1	VNG1066C					407:414	VNG1066C	407:414	VNG1066C	407:414	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	2	49	dep	glycosyltransferases	376:395	arg1	VNG1062G					421:428	VNG1062G	421:428	VNG1062G	421:428	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	2	49	dep	glycosyltransferases	376:395	arg1	VNG1067G					397:404	VNG1067G	397:404	VNG1067G	397:404	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	4	50	theme	phosphate	754:762	arg1	pool					737:740	the pool	733:740	the pool of dolichol phosphate, the lipid upon which this glycan is assembled,	733:810	Likewise, the pool of dolichol phosphate, the lipid upon which this glycan is assembled, was also considered in each deletion strain.
34925283	2	51	gly	glycoproteins	488:500	arg1	glycoproteins					488:500	glycoproteins	488:500	glycoproteins in this species	488:516	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	2	52	theme	delineated	274:283	arg1	pathways					310:317	delineated archaeal N-glycosylation pathways	274:317	delineated archaeal N-glycosylation pathways	274:317	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	6	53	theme	N-linked	1226:1233	arg1	tetrasaccharide					1235:1249	the N-linked tetrasaccharide	1222:1249	the N-linked tetrasaccharide decorating Hbt	1222:1264	The results revealed that VNG1067G, VNG1066C, and VNG1062G, renamed Agl25, Agl26, and Agl27 according to the nomenclature used for archaeal N-glycosylation pathway components, are responsible for adding the second, third and fourth sugars of the N-linked tetrasaccharide decorating Hbt.
34925283	2	54	theme	Halobacterium	352:364	arg1	glycosyltransferases					376:395	the putative Halobacterium salinarum glycosyltransferases	339:395	the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G	339:428	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	2	54	theme	Halobacterium	352:364	arg1	VNG1066C					407:414	VNG1066C	407:414	VNG1066C	407:414	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	2	54	theme	Halobacterium	352:364	arg1	VNG1062G					421:428	VNG1062G	421:428	VNG1062G	421:428	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	2	54	theme	Halobacterium	352:364	arg1	VNG1067G					397:404	VNG1067G	397:404	VNG1067G	397:404	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	1	55	from	process	126:132	arg1	Archaea					137:143	Archaea	137:143	Archaea	137:143	Whereas N-glycosylation is a seemingly universal process in Archaea, pathways of N-glycosylation have only been experimentally verified in a mere handful of species.
34925283	1	56	theme	species	234:240	arg1	handful					223:229	a mere handful	216:229	a mere handful of species	216:240	Whereas N-glycosylation is a seemingly universal process in Archaea, pathways of N-glycosylation have only been experimentally verified in a mere handful of species.
34925283	7	57	gly	glycoproteins	1277:1289	arg1	glycoproteins					1277:1289	salinarum glycoproteins	1267:1289	salinarum glycoproteins	1267:1289	salinarum glycoproteins.
34925283	2	58	link	N-linked	452:459	arg1	tetrasaccharide					461:475	an N-linked tetrasaccharide	449:475	an N-linked tetrasaccharide decorating glycoproteins in this species	449:516	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	6	59	theme	fourth	1205:1210	arg1	sugars					1212:1217	the second, third and fourth sugars	1183:1217	the second, third and fourth sugars of the N-linked tetrasaccharide decorating Hbt	1183:1264	The results revealed that VNG1067G, VNG1066C, and VNG1062G, renamed Agl25, Agl26, and Agl27 according to the nomenclature used for archaeal N-glycosylation pathway components, are responsible for adding the second, third and fourth sugars of the N-linked tetrasaccharide decorating Hbt.
34925283	2	60	theme	putative	343:350	arg1	glycosyltransferases					376:395	the putative Halobacterium salinarum glycosyltransferases	339:395	the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G	339:428	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	2	60	theme	putative	343:350	arg1	VNG1066C					407:414	VNG1066C	407:414	VNG1066C	407:414	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	2	60	theme	putative	343:350	arg1	VNG1062G					421:428	VNG1062G	421:428	VNG1062G	421:428	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	2	60	theme	putative	343:350	arg1	VNG1067G					397:404	VNG1067G	397:404	VNG1067G	397:404	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
34925283	6	61	theme	N-glycosylation	1120:1134	arg1	components					1144:1153	archaeal N-glycosylation pathway components	1111:1153	archaeal N-glycosylation pathway components	1111:1153	The results revealed that VNG1067G, VNG1066C, and VNG1062G, renamed Agl25, Agl26, and Agl27 according to the nomenclature used for archaeal N-glycosylation pathway components, are responsible for adding the second, third and fourth sugars of the N-linked tetrasaccharide decorating Hbt.
34925283	3	62	theme	glycoproteins	656:668	arg1	impact					579:584	the impact	575:584	the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism,	575:686	Following deletion of each encoding gene, the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism, was assessed by mass spectrometry.
34925283	3	63	theme	gene	569:572	arg1	deletion					543:550	deletion	543:550	deletion of each encoding gene	543:572	Following deletion of each encoding gene, the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism, was assessed by mass spectrometry.
34925283	3	64	from	glycoprotein	620:631	arg1	organism					678:685	this organism	673:685	this organism	673:685	Following deletion of each encoding gene, the impact on N-glycosylation of the S-layer glycoprotein and archaellins, major glycoproteins in this organism, was assessed by mass spectrometry.
34925283	8	65	gly	N-glycosylation	1342:1356	arg1	Hbt					1384:1386	Hbt	1384:1386	Hbt	1384:1386	Moreover, this study demonstrated how compromised N-glycosylation affects various facets of Hbt.
34925283	6	66	theme	archaeal	1111:1118	arg1	components					1144:1153	archaeal N-glycosylation pathway components	1111:1153	archaeal N-glycosylation pathway components	1111:1153	The results revealed that VNG1067G, VNG1066C, and VNG1062G, renamed Agl25, Agl26, and Agl27 according to the nomenclature used for archaeal N-glycosylation pathway components, are responsible for adding the second, third and fourth sugars of the N-linked tetrasaccharide decorating Hbt.
34925283	1	67	theme	N-glycosylation	158:172	arg1	pathways					146:153	pathways	146:153	pathways of N-glycosylation	146:172	Whereas N-glycosylation is a seemingly universal process in Archaea, pathways of N-glycosylation have only been experimentally verified in a mere handful of species.
34925283	2	68	from	involvement	324:334	arg1	assembly					437:444	the assembly	433:444	the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species	433:516	Toward expanding the number of delineated archaeal N-glycosylation pathways, the involvement of the putative Halobacterium salinarum glycosyltransferases VNG1067G, VNG1066C, and VNG1062G in the assembly of an N-linked tetrasaccharide decorating glycoproteins in this species was addressed.
32507236	6	0	from	glycans	999:1005	arg1	Asn112					1054:1059	Asn112	1054:1059	Asn112	1054:1059	Our results revealed 10 glycan compositions for salivary AZGP1, including core fucosylated glycans on Asn128 and sialylated glycans on Asn109 and Asn112.
32507236	6	0	from	glycans	999:1005	arg1	Asn109					1043:1048	Asn109	1043:1048	Asn109	1043:1048	Our results revealed 10 glycan compositions for salivary AZGP1, including core fucosylated glycans on Asn128 and sialylated glycans on Asn109 and Asn112.
32507236	6	0	from	glycans	999:1005	arg1	Asn128					1010:1015	Asn128	1010:1015	Asn128	1010:1015	Our results revealed 10 glycan compositions for salivary AZGP1, including core fucosylated glycans on Asn128 and sialylated glycans on Asn109 and Asn112.
32507236	6	1	theme	fucosylated	987:997	arg1	glycans					999:1005	core fucosylated glycans	982:1005	core fucosylated glycans on Asn128	982:1015	Our results revealed 10 glycan compositions for salivary AZGP1, including core fucosylated glycans on Asn128 and sialylated glycans on Asn109 and Asn112.
32507236	1	2	theme	glycan	289:294	arg1	structures					296:305	their uncharacterized glycan structures	267:305	their uncharacterized glycan structures	267:305	Glycoproteins are important biomarkers for cancers, while most glycoproteomics biomarkers suffering from low sensitivity and specificity due to their uncharacterized glycan structures.
32507236	6	3	theme	core	982:985	arg1	glycans					999:1005	core fucosylated glycans	982:1005	core fucosylated glycans on Asn128	982:1015	Our results revealed 10 glycan compositions for salivary AZGP1, including core fucosylated glycans on Asn128 and sialylated glycans on Asn109 and Asn112.
32507236	1	4	theme	most	181:184	arg1	biomarkers					202:211	most glycoproteomics biomarkers	181:211	most glycoproteomics biomarkers suffering from low sensitivity and specificity due to their uncharacterized glycan structures	181:305	Glycoproteins are important biomarkers for cancers, while most glycoproteomics biomarkers suffering from low sensitivity and specificity due to their uncharacterized glycan structures.
32507236	11	5	theme	glycan	1596:1601	arg1	structures					1603:1612	glycan structures	1596:1612	glycan structures	1596:1612	Our developed strategy holds promise for thorough identification of glycan structures on a target glycoprotein biomarker.
32507236	9	6	theme	potential	1296:1304	arg1	structures					1313:1322	22 potential glycan structures	1293:1322	22 potential glycan structures	1293:1322	In total, 15 glycan compositions and 22 potential glycan structures were identified and characterized for AZGP1, including some different structures with the same compositions.
32507236	9	7	theme	glycan	1269:1274	arg1	compositions					1276:1287	15 glycan compositions	1266:1287	15 glycan compositions	1266:1287	In total, 15 glycan compositions and 22 potential glycan structures were identified and characterized for AZGP1, including some different structures with the same compositions.
32507236	2	8	used	used	389:392	arg2	AZGP1					308:312	AZGP1	308:312	AZGP1	308:312	AZGP1 is a potential biomarker for salivary diagnostics of lung cancer, which is used as a model glycoprotein in this study for method development.
32507236	2	8	used	used	389:392	arg2	biomarker					329:337	a potential biomarker	317:337	a potential biomarker	317:337	AZGP1 is a potential biomarker for salivary diagnostics of lung cancer, which is used as a model glycoprotein in this study for method development.
32507236	2	8	used	used	389:392	arg2	glycoprotein					405:416	a model glycoprotein	397:416	a model glycoprotein in this study	397:430	AZGP1 is a potential biomarker for salivary diagnostics of lung cancer, which is used as a model glycoprotein in this study for method development.
32507236	4	9	theme	western	726:732	arg1	blot					734:737	two-dimensional western blot	710:737	two-dimensional western blot as well as mass spectrometry	710:766	7 gel spots of AZGP1 were resolved by two-dimensional gel electrophoresis and further confirmed by two-dimensional western blot as well as mass spectrometry.
32507236	7	10	from	group	1139:1143	arg1	structures					1093:1102	the glycan structures	1082:1102	the glycan structures of salivary AZGP1 from lung cancer group and control group	1082:1161	We further compared the glycan structures of salivary AZGP1 from lung cancer group and control group.
32507236	0	11	theme	mass	104:107	arg1	spectrometry					109:120	mass spectrometry	104:120	mass spectrometry	104:120	N-glycan structures of target cancer biomarker characterized by two-dimensional gel electrophoresis and mass spectrometry.
32507236	3	12	gly	300 N-glycoproteins	557:575	arg1	300 N-glycoproteins					557:575	300 N-glycoproteins	557:575	300 N-glycoproteins	557:575	We initially analyzed salivary N-glycoproteome by using lectin affinity chromatography and more than 300 N-glycoproteins were identified, including AZGP1.
32507236	10	13	theme	glycan	1460:1465	arg1	structures					1467:1476	5 potential glycan structures	1448:1476	5 potential glycan structures	1448:1476	In particular, 5 potential glycan structures were identified as lung cancer unique signatures.
32507236	10	13	theme	glycan	1460:1465	arg1	signatures					1516:1525	lung cancer unique signatures	1497:1525	lung cancer unique signatures	1497:1525	In particular, 5 potential glycan structures were identified as lung cancer unique signatures.
32507236	2	14	theme	potential	319:327	arg1	biomarker					329:337	a potential biomarker	317:337	a potential biomarker	317:337	AZGP1 is a potential biomarker for salivary diagnostics of lung cancer, which is used as a model glycoprotein in this study for method development.
32507236	2	14	theme	potential	319:327	arg1	glycoprotein					405:416	a model glycoprotein	397:416	a model glycoprotein in this study	397:430	AZGP1 is a potential biomarker for salivary diagnostics of lung cancer, which is used as a model glycoprotein in this study for method development.
32507236	2	14	theme	potential	319:327	arg1	AZGP1					308:312	AZGP1	308:312	AZGP1	308:312	AZGP1 is a potential biomarker for salivary diagnostics of lung cancer, which is used as a model glycoprotein in this study for method development.
32507236	3	15	theme	affinity	519:526	arg1	chromatography					528:541	lectin affinity chromatography	512:541	lectin affinity chromatography	512:541	We initially analyzed salivary N-glycoproteome by using lectin affinity chromatography and more than 300 N-glycoproteins were identified, including AZGP1.
32507236	5	16	theme	AZGP1	803:807	arg1	structures					789:798	The isomeric glycan structures	769:798	The isomeric glycan structures of AZGP1 in these spots	769:822	The isomeric glycan structures of AZGP1 in these spots were systematically characterized both at composition level and at structure level.
32507236	9	17	theme	different	1384:1392	arg1	structures					1394:1403	some different structures	1379:1403	some different structures with the same compositions	1379:1430	In total, 15 glycan compositions and 22 potential glycan structures were identified and characterized for AZGP1, including some different structures with the same compositions.
32507236	10	18	theme	potential	1450:1458	arg1	structures					1467:1476	5 potential glycan structures	1448:1476	5 potential glycan structures	1448:1476	In particular, 5 potential glycan structures were identified as lung cancer unique signatures.
32507236	10	18	theme	potential	1450:1458	arg1	signatures					1516:1525	lung cancer unique signatures	1497:1525	lung cancer unique signatures	1497:1525	In particular, 5 potential glycan structures were identified as lung cancer unique signatures.
32507236	1	19	theme	glycoproteomics	186:200	arg1	biomarkers					202:211	most glycoproteomics biomarkers	181:211	most glycoproteomics biomarkers suffering from low sensitivity and specificity due to their uncharacterized glycan structures	181:305	Glycoproteins are important biomarkers for cancers, while most glycoproteomics biomarkers suffering from low sensitivity and specificity due to their uncharacterized glycan structures.
32507236	6	20	theme	salivary	956:963	arg1	AZGP1					965:969	salivary AZGP1	956:969	salivary AZGP1	956:969	Our results revealed 10 glycan compositions for salivary AZGP1, including core fucosylated glycans on Asn128 and sialylated glycans on Asn109 and Asn112.
32507236	4	21	theme	AZGP1	626:630	arg1	spots					617:621	7 gel spots	611:621	7 gel spots of AZGP1	611:630	7 gel spots of AZGP1 were resolved by two-dimensional gel electrophoresis and further confirmed by two-dimensional western blot as well as mass spectrometry.
32507236	10	22	theme	cancer	1502:1507	arg1	structures					1467:1476	5 potential glycan structures	1448:1476	5 potential glycan structures	1448:1476	In particular, 5 potential glycan structures were identified as lung cancer unique signatures.
32507236	10	22	theme	cancer	1502:1507	arg1	signatures					1516:1525	lung cancer unique signatures	1497:1525	lung cancer unique signatures	1497:1525	In particular, 5 potential glycan structures were identified as lung cancer unique signatures.
32507236	12	23	theme	In-depth	1650:1657	arg1	characterization					1659:1674	In-depth characterization	1650:1674	In-depth characterization of its glycan structures	1650:1699	In-depth characterization of its glycan structures will ultimately enhance its sensitivity and specificity for cancer detection.
32507236	9	24	theme	glycan	1306:1311	arg1	structures					1313:1322	22 potential glycan structures	1293:1322	22 potential glycan structures	1293:1322	In total, 15 glycan compositions and 22 potential glycan structures were identified and characterized for AZGP1, including some different structures with the same compositions.
32507236	4	25	theme	two-dimensional	710:724	arg1	blot					734:737	two-dimensional western blot	710:737	two-dimensional western blot as well as mass spectrometry	710:766	7 gel spots of AZGP1 were resolved by two-dimensional gel electrophoresis and further confirmed by two-dimensional western blot as well as mass spectrometry.
32507236	7	26	theme	control	1149:1155	arg1	group					1157:1161	control group	1149:1161	control group	1149:1161	We further compared the glycan structures of salivary AZGP1 from lung cancer group and control group.
32507236	8	27	theme	glycan	1196:1201	arg1	structures					1203:1212	14 and 7 potential glycan structures	1177:1212	14 and 7 potential glycan structures	1177:1212	Accordingly, 14 and 7 potential glycan structures were successfully revealed, respectively.
32507236	0	28	theme	N-glycan	0:7	arg1	structures					9:18	N-glycan structures	0:18	N-glycan structures of target cancer biomarker	0:45	N-glycan structures of target cancer biomarker characterized by two-dimensional gel electrophoresis and mass spectrometry.
32507236	10	29	theme	unique	1509:1514	arg1	structures					1467:1476	5 potential glycan structures	1448:1476	5 potential glycan structures	1448:1476	In particular, 5 potential glycan structures were identified as lung cancer unique signatures.
32507236	10	29	theme	unique	1509:1514	arg1	signatures					1516:1525	lung cancer unique signatures	1497:1525	lung cancer unique signatures	1497:1525	In particular, 5 potential glycan structures were identified as lung cancer unique signatures.
32507236	2	30	gly	glycoprotein	405:416	arg1	biomarker					329:337	a potential biomarker	317:337	a potential biomarker	317:337	AZGP1 is a potential biomarker for salivary diagnostics of lung cancer, which is used as a model glycoprotein in this study for method development.
32507236	2	30	gly	glycoprotein	405:416	arg1	glycoprotein					405:416	a model glycoprotein	397:416	a model glycoprotein in this study	397:430	AZGP1 is a potential biomarker for salivary diagnostics of lung cancer, which is used as a model glycoprotein in this study for method development.
32507236	2	31	theme	model	399:403	arg1	biomarker					329:337	a potential biomarker	317:337	a potential biomarker	317:337	AZGP1 is a potential biomarker for salivary diagnostics of lung cancer, which is used as a model glycoprotein in this study for method development.
32507236	2	31	theme	model	399:403	arg1	glycoprotein					405:416	a model glycoprotein	397:416	a model glycoprotein in this study	397:430	AZGP1 is a potential biomarker for salivary diagnostics of lung cancer, which is used as a model glycoprotein in this study for method development.
32507236	0	32	theme	target	23:28	arg1	biomarker					37:45	target cancer biomarker	23:45	target cancer biomarker	23:45	N-glycan structures of target cancer biomarker characterized by two-dimensional gel electrophoresis and mass spectrometry.
32507236	7	33	from	group	1157:1161	arg1	structures					1093:1102	the glycan structures	1082:1102	the glycan structures of salivary AZGP1 from lung cancer group and control group	1082:1161	We further compared the glycan structures of salivary AZGP1 from lung cancer group and control group.
32507236	11	34	theme	glycoprotein	1626:1637	arg1	biomarker					1639:1647	a target glycoprotein biomarker	1617:1647	a target glycoprotein biomarker	1617:1647	Our developed strategy holds promise for thorough identification of glycan structures on a target glycoprotein biomarker.
32507236	2	35	theme	salivary	343:350	arg1	diagnostics					352:362	salivary diagnostics	343:362	salivary diagnostics of lung cancer	343:377	AZGP1 is a potential biomarker for salivary diagnostics of lung cancer, which is used as a model glycoprotein in this study for method development.
32507236	1	36	theme	low	228:230	arg1	sensitivity					232:242	low sensitivity	228:242	low sensitivity	228:242	Glycoproteins are important biomarkers for cancers, while most glycoproteomics biomarkers suffering from low sensitivity and specificity due to their uncharacterized glycan structures.
32507236	5	37	theme	structure	891:899	arg1	level					901:905	structure level	891:905	structure level	891:905	The isomeric glycan structures of AZGP1 in these spots were systematically characterized both at composition level and at structure level.
32507236	7	38	theme	lung	1127:1130	arg1	group					1139:1143	lung cancer group	1127:1143	lung cancer group	1127:1143	We further compared the glycan structures of salivary AZGP1 from lung cancer group and control group.
32507236	6	39	from	glycans	1032:1038	arg1	Asn112					1054:1059	Asn112	1054:1059	Asn112	1054:1059	Our results revealed 10 glycan compositions for salivary AZGP1, including core fucosylated glycans on Asn128 and sialylated glycans on Asn109 and Asn112.
32507236	6	39	from	glycans	1032:1038	arg1	Asn109					1043:1048	Asn109	1043:1048	Asn109	1043:1048	Our results revealed 10 glycan compositions for salivary AZGP1, including core fucosylated glycans on Asn128 and sialylated glycans on Asn109 and Asn112.
32507236	6	39	from	glycans	1032:1038	arg1	Asn128					1010:1015	Asn128	1010:1015	Asn128	1010:1015	Our results revealed 10 glycan compositions for salivary AZGP1, including core fucosylated glycans on Asn128 and sialylated glycans on Asn109 and Asn112.
32507236	4	40	theme	mass	750:753	arg1	spectrometry					755:766	mass spectrometry	750:766	two-dimensional western blot as well as mass spectrometry	710:766	7 gel spots of AZGP1 were resolved by two-dimensional gel electrophoresis and further confirmed by two-dimensional western blot as well as mass spectrometry.
32507236	11	41	theme	developed	1532:1540	arg1	strategy					1542:1549	Our developed strategy	1528:1549	Our developed strategy	1528:1549	Our developed strategy holds promise for thorough identification of glycan structures on a target glycoprotein biomarker.
32507236	2	42	theme	method	436:441	arg1	development					443:453	method development	436:453	method development	436:453	AZGP1 is a potential biomarker for salivary diagnostics of lung cancer, which is used as a model glycoprotein in this study for method development.
32507236	0	43	theme	biomarker	37:45	arg1	structures					9:18	N-glycan structures	0:18	N-glycan structures of target cancer biomarker	0:45	N-glycan structures of target cancer biomarker characterized by two-dimensional gel electrophoresis and mass spectrometry.
32507236	4	44	theme	gel	613:615	arg1	spots					617:621	7 gel spots	611:621	7 gel spots of AZGP1	611:630	7 gel spots of AZGP1 were resolved by two-dimensional gel electrophoresis and further confirmed by two-dimensional western blot as well as mass spectrometry.
32507236	5	45	theme	isomeric	773:780	arg1	structures					789:798	The isomeric glycan structures	769:798	The isomeric glycan structures of AZGP1 in these spots	769:822	The isomeric glycan structures of AZGP1 in these spots were systematically characterized both at composition level and at structure level.
32507236	6	46	theme	glycan	932:937	arg1	glycans					999:1005	core fucosylated glycans	982:1005	core fucosylated glycans on Asn128	982:1015	Our results revealed 10 glycan compositions for salivary AZGP1, including core fucosylated glycans on Asn128 and sialylated glycans on Asn109 and Asn112.
32507236	6	46	theme	glycan	932:937	arg1	glycans					1032:1038	sialylated glycans	1021:1038	sialylated glycans on Asn109 and Asn112	1021:1059	Our results revealed 10 glycan compositions for salivary AZGP1, including core fucosylated glycans on Asn128 and sialylated glycans on Asn109 and Asn112.
32507236	6	46	theme	glycan	932:937	arg1	compositions					939:950	10 glycan compositions	929:950	10 glycan compositions	929:950	Our results revealed 10 glycan compositions for salivary AZGP1, including core fucosylated glycans on Asn128 and sialylated glycans on Asn109 and Asn112.
32507236	11	47	gly	glycoprotein	1626:1637	arg1	glycoprotein					1626:1637	a target glycoprotein biomarker	1617:1647	a target glycoprotein biomarker	1617:1647	Our developed strategy holds promise for thorough identification of glycan structures on a target glycoprotein biomarker.
32507236	12	48	theme	cancer	1761:1766	arg1	detection					1768:1776	cancer detection	1761:1776	cancer detection	1761:1776	In-depth characterization of its glycan structures will ultimately enhance its sensitivity and specificity for cancer detection.
32507236	3	49	theme	salivary	478:485	arg1	N-glycoproteome					487:501	salivary N-glycoproteome	478:501	salivary N-glycoproteome	478:501	We initially analyzed salivary N-glycoproteome by using lectin affinity chromatography and more than 300 N-glycoproteins were identified, including AZGP1.
32507236	0	50	theme	cancer	30:35	arg1	biomarker					37:45	target cancer biomarker	23:45	target cancer biomarker	23:45	N-glycan structures of target cancer biomarker characterized by two-dimensional gel electrophoresis and mass spectrometry.
32507236	6	51	theme	sialylated	1021:1030	arg1	glycans					1032:1038	sialylated glycans	1021:1038	sialylated glycans on Asn109 and Asn112	1021:1059	Our results revealed 10 glycan compositions for salivary AZGP1, including core fucosylated glycans on Asn128 and sialylated glycans on Asn109 and Asn112.
32507236	5	52	theme	glycan	782:787	arg1	structures					789:798	The isomeric glycan structures	769:798	The isomeric glycan structures of AZGP1 in these spots	769:822	The isomeric glycan structures of AZGP1 in these spots were systematically characterized both at composition level and at structure level.
32507236	5	53	from	structures	789:798	arg1	spots					818:822	these spots	812:822	these spots	812:822	The isomeric glycan structures of AZGP1 in these spots were systematically characterized both at composition level and at structure level.
32507236	9	54	theme	same	1414:1417	arg1	compositions					1419:1430	the same compositions	1410:1430	the same compositions	1410:1430	In total, 15 glycan compositions and 22 potential glycan structures were identified and characterized for AZGP1, including some different structures with the same compositions.
32507236	2	55	from	glycoprotein	405:416	arg1	study					426:430	this study	421:430	this study	421:430	AZGP1 is a potential biomarker for salivary diagnostics of lung cancer, which is used as a model glycoprotein in this study for method development.
32507236	10	56	theme	lung	1497:1500	arg1	structures					1467:1476	5 potential glycan structures	1448:1476	5 potential glycan structures	1448:1476	In particular, 5 potential glycan structures were identified as lung cancer unique signatures.
32507236	10	56	theme	lung	1497:1500	arg1	signatures					1516:1525	lung cancer unique signatures	1497:1525	lung cancer unique signatures	1497:1525	In particular, 5 potential glycan structures were identified as lung cancer unique signatures.
32507236	12	57	theme	structures	1690:1699	arg1	characterization					1659:1674	In-depth characterization	1650:1674	In-depth characterization of its glycan structures	1650:1699	In-depth characterization of its glycan structures will ultimately enhance its sensitivity and specificity for cancer detection.
32507236	7	58	theme	AZGP1	1116:1120	arg1	structures					1093:1102	the glycan structures	1082:1102	the glycan structures of salivary AZGP1 from lung cancer group and control group	1082:1161	We further compared the glycan structures of salivary AZGP1 from lung cancer group and control group.
32507236	8	59	theme	potential	1186:1194	arg1	structures					1203:1212	14 and 7 potential glycan structures	1177:1212	14 and 7 potential glycan structures	1177:1212	Accordingly, 14 and 7 potential glycan structures were successfully revealed, respectively.
32507236	7	60	theme	cancer	1132:1137	arg1	group					1139:1143	lung cancer group	1127:1143	lung cancer group	1127:1143	We further compared the glycan structures of salivary AZGP1 from lung cancer group and control group.
32507236	5	61	theme	composition	866:876	arg1	level					878:882	composition level	866:882	composition level	866:882	The isomeric glycan structures of AZGP1 in these spots were systematically characterized both at composition level and at structure level.
32507236	1	62	theme	important	141:149	arg1	biomarkers					151:160	important biomarkers	141:160	important biomarkers	141:160	Glycoproteins are important biomarkers for cancers, while most glycoproteomics biomarkers suffering from low sensitivity and specificity due to their uncharacterized glycan structures.
32507236	1	62	theme	important	141:149	arg1	Glycoproteins					123:135	Glycoproteins	123:135	Glycoproteins	123:135	Glycoproteins are important biomarkers for cancers, while most glycoproteomics biomarkers suffering from low sensitivity and specificity due to their uncharacterized glycan structures.
32507236	9	63	with	structures	1394:1403	arg1	compositions					1419:1430	the same compositions	1410:1430	the same compositions	1410:1430	In total, 15 glycan compositions and 22 potential glycan structures were identified and characterized for AZGP1, including some different structures with the same compositions.
32507236	11	64	theme	target	1619:1624	arg1	biomarker					1639:1647	a target glycoprotein biomarker	1617:1647	a target glycoprotein biomarker	1617:1647	Our developed strategy holds promise for thorough identification of glycan structures on a target glycoprotein biomarker.
32507236	6	65	gly	sialylated	1021:1030	arg1	glycans					1032:1038	sialylated glycans	1021:1038	sialylated glycans on Asn109 and Asn112	1021:1059	Our results revealed 10 glycan compositions for salivary AZGP1, including core fucosylated glycans on Asn128 and sialylated glycans on Asn109 and Asn112.
32507236	2	66	theme	cancer	372:377	arg1	diagnostics					352:362	salivary diagnostics	343:362	salivary diagnostics of lung cancer	343:377	AZGP1 is a potential biomarker for salivary diagnostics of lung cancer, which is used as a model glycoprotein in this study for method development.
32507236	4	67	theme	two-dimensional	649:663	arg1	electrophoresis					669:683	two-dimensional gel electrophoresis	649:683	two-dimensional gel electrophoresis	649:683	7 gel spots of AZGP1 were resolved by two-dimensional gel electrophoresis and further confirmed by two-dimensional western blot as well as mass spectrometry.
32507236	7	68	theme	salivary	1107:1114	arg1	AZGP1					1116:1120	salivary AZGP1	1107:1120	salivary AZGP1	1107:1120	We further compared the glycan structures of salivary AZGP1 from lung cancer group and control group.
32507236	7	69	theme	glycan	1086:1091	arg1	structures					1093:1102	the glycan structures	1082:1102	the glycan structures of salivary AZGP1 from lung cancer group and control group	1082:1161	We further compared the glycan structures of salivary AZGP1 from lung cancer group and control group.
32507236	12	70	theme	glycan	1683:1688	arg1	structures					1690:1699	its glycan structures	1679:1699	its glycan structures	1679:1699	In-depth characterization of its glycan structures will ultimately enhance its sensitivity and specificity for cancer detection.
32507236	2	71	theme	lung	367:370	arg1	cancer					372:377	lung cancer	367:377	lung cancer	367:377	AZGP1 is a potential biomarker for salivary diagnostics of lung cancer, which is used as a model glycoprotein in this study for method development.
32507236	3	72	theme	lectin	512:517	arg1	chromatography					528:541	lectin affinity chromatography	512:541	lectin affinity chromatography	512:541	We initially analyzed salivary N-glycoproteome by using lectin affinity chromatography and more than 300 N-glycoproteins were identified, including AZGP1.
32507236	0	73	theme	gel	80:82	arg1	electrophoresis					84:98	two-dimensional gel electrophoresis	64:98	two-dimensional gel electrophoresis	64:98	N-glycan structures of target cancer biomarker characterized by two-dimensional gel electrophoresis and mass spectrometry.
32507236	11	74	theme	structures	1603:1612	arg1	identification					1578:1591	thorough identification	1569:1591	thorough identification of glycan structures	1569:1612	Our developed strategy holds promise for thorough identification of glycan structures on a target glycoprotein biomarker.
32507236	11	75	theme	thorough	1569:1576	arg1	identification					1578:1591	thorough identification	1569:1591	thorough identification of glycan structures	1569:1612	Our developed strategy holds promise for thorough identification of glycan structures on a target glycoprotein biomarker.
32507236	0	76	theme	two-dimensional	64:78	arg1	electrophoresis					84:98	two-dimensional gel electrophoresis	64:98	two-dimensional gel electrophoresis	64:98	N-glycan structures of target cancer biomarker characterized by two-dimensional gel electrophoresis and mass spectrometry.
32507236	4	77	theme	gel	665:667	arg1	electrophoresis					669:683	two-dimensional gel electrophoresis	649:683	two-dimensional gel electrophoresis	649:683	7 gel spots of AZGP1 were resolved by two-dimensional gel electrophoresis and further confirmed by two-dimensional western blot as well as mass spectrometry.
32507236	6	78	gly	fucosylated	987:997	arg1	glycans					999:1005	core fucosylated glycans	982:1005	core fucosylated glycans on Asn128	982:1015	Our results revealed 10 glycan compositions for salivary AZGP1, including core fucosylated glycans on Asn128 and sialylated glycans on Asn109 and Asn112.
32507236	1	79	theme	uncharacterized	273:287	arg1	structures					296:305	their uncharacterized glycan structures	267:305	their uncharacterized glycan structures	267:305	Glycoproteins are important biomarkers for cancers, while most glycoproteomics biomarkers suffering from low sensitivity and specificity due to their uncharacterized glycan structures.
32084489	5	0	theme	sugar	744:748	arg1	residues					750:757	other sugar residues	738:757	other sugar residues	738:757	Structural analyses demonstrated that the dominate components in JUYP were 6-β-D-Glcp, and other sugar residues included 2,4-β-D-Manp and T-β-D-Galf.
32084489	6	1	theme	entangled	866:874	arg1	chains					876:881	entangled chains	866:881	entangled chains	866:881	TEM images further revealed JUYP was a linear branched molecule with entangled chains.
32084489	5	2	from	components	698:707	arg1	JUYP					712:715	JUYP	712:715	JUYP	712:715	Structural analyses demonstrated that the dominate components in JUYP were 6-β-D-Glcp, and other sugar residues included 2,4-β-D-Manp and T-β-D-Galf.
32084489	7	3	theme	inhibitory	954:963	arg1	effects					965:971	good inhibitory effects	949:971	good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells	949:1054	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	6	4	theme	linear	836:841	arg1	molecule					852:859	a linear branched molecule	834:859	a linear branched molecule with entangled chains	834:881	TEM images further revealed JUYP was a linear branched molecule with entangled chains.
32084489	7	5	theme	IL-1β	1134:1138	arg1	expressions					1140:1150	IL-1β expressions	1134:1150	IL-1β expressions	1134:1150	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	7	6	theme	JUYP	934:937	arg1	1 μg/mL					923:929	1 μg/mL	923:929	1 μg/mL of JUYP	923:937	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	7	7	from	IL-6	983:986	arg1	cells					1050:1054	LPS-stimulated RAW 264.7 cells	1025:1054	LPS-stimulated RAW 264.7 cells	1025:1054	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	6	8	theme	TEM	797:799	arg1	images					801:806	TEM images	797:806	TEM images	797:806	TEM images further revealed JUYP was a linear branched molecule with entangled chains.
32084489	7	9	theme	JUYP	1126:1129	arg1	effects					1078:1084	the inhibitory effects	1063:1084	the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions	1063:1150	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	7	9	theme	JUYP	1126:1129	arg1	significant					1162:1172	significant	1162:1172	significant	1162:1172	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	7	10	theme	inhibitory	1067:1076	arg1	effects					1078:1084	the inhibitory effects	1063:1084	the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions	1063:1150	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	7	10	theme	inhibitory	1067:1076	arg1	significant					1162:1172	significant	1162:1172	significant	1162:1172	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	7	11	theme	good	949:952	arg1	effects					965:971	good inhibitory effects	949:971	good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells	949:1054	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	2	12	dep	Fourier	250:256	arg1	transform					258:266	transform	258:266	transform infrared spectroscopy (FTIR), methylation-GC-MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM)	258:391	The detailed structure of JUYP was studied using gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR), methylation-GC-MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM).
32084489	7	13	dep	effects	1078:1084	arg1	%					1108:1108	55.89%	1103:1108	55.89% for protein	1103:1120	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	7	13	dep	effects	1078:1084	arg1	%					1091:1091	87.8%	1087:1091	87.8% for mRNA	1087:1100	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	3	14	theme	577 kDa	502:508	arg1	yield					450:454	the yield	446:454	the yield of 21.2%	446:463	A homogeneous polysaccharide JUYP was obtained with the yield of 21.2% and average molecular weight (Mw) of 577 kDa.
32084489	3	14	theme	577 kDa	502:508	arg1	Mw					495:496	Mw	495:496	Mw	495:496	A homogeneous polysaccharide JUYP was obtained with the yield of 21.2% and average molecular weight (Mw) of 577 kDa.
32084489	3	14	theme	577 kDa	502:508	arg1	weight					487:492	average molecular weight	469:492	average molecular weight (Mw) of 577 kDa	469:508	A homogeneous polysaccharide JUYP was obtained with the yield of 21.2% and average molecular weight (Mw) of 577 kDa.
32084489	7	15	from	TNF-α	976:980	arg1	cells					1050:1054	LPS-stimulated RAW 264.7 cells	1025:1054	LPS-stimulated RAW 264.7 cells	1025:1054	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	0	16	from	yunnana	34:40	arg1	polysaccharide					2:15	A polysaccharide	0:15	A polysaccharide from Umbilicaria yunnana: Structural characterization and anti-inflammation effects.	0:100	A polysaccharide from Umbilicaria yunnana: Structural characterization and anti-inflammation effects.
32084489	5	17	dep	components	698:707	arg1	dominate					689:696	dominate	689:696	dominate	689:696	Structural analyses demonstrated that the dominate components in JUYP were 6-β-D-Glcp, and other sugar residues included 2,4-β-D-Manp and T-β-D-Galf.
32084489	2	18	theme	nuclear	317:323	arg1	NMR					345:347	NMR	345:347	NMR	345:347	The detailed structure of JUYP was studied using gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR), methylation-GC-MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM).
32084489	2	18	theme	nuclear	317:323	arg1	resonance					334:342	nuclear magnetic resonance	317:342	nuclear magnetic resonance (NMR)	317:348	The detailed structure of JUYP was studied using gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR), methylation-GC-MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM).
32084489	1	19	theme	polysaccharide	104:117	arg1	JUYP					119:122	A polysaccharide JUYP	102:122	A polysaccharide JUYP	102:122	A polysaccharide JUYP was isolated and purified from Umbilicaria yunnana.
32084489	2	20	dep	transform	258:266	arg1	infrared					268:275	infrared	268:275	transform infrared spectroscopy (FTIR), methylation-GC-MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM)	258:391	The detailed structure of JUYP was studied using gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR), methylation-GC-MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM).
32084489	0	21	theme	Umbilicaria	22:32	arg1	yunnana					34:40	Umbilicaria yunnana	22:40	Umbilicaria yunnana	22:40	A polysaccharide from Umbilicaria yunnana: Structural characterization and anti-inflammation effects.
32084489	4	22	theme	composition	526:536	arg1	analysis					538:545	Monosaccharide composition analysis	511:545	Monosaccharide composition analysis	511:545	Monosaccharide composition analysis indicated that JUYP was composed of glucose, galactose and mannose with a molar ratio of 2.3:1:0.7.
32084489	7	23	dep	dexamethasone	1187:1199	arg1	%					1229:1229	35.15%	1224:1229	35.15% for protein	1224:1241	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	7	23	dep	dexamethasone	1187:1199	arg1	DXMS					1202:1205	DXMS	1202:1205	DXMS	1202:1205	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	7	23	dep	dexamethasone	1187:1199	arg1	%					1212:1212	61.6%	1208:1212	61.6% for mRNA	1208:1221	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	2	24	theme	JUYP	202:205	arg1	structure					189:197	The detailed structure	176:197	The detailed structure of JUYP	176:205	The detailed structure of JUYP was studied using gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR), methylation-GC-MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM).
32084489	7	25	from	effects	965:971	arg1	IL-1β					989:993	IL-1β	989:993	IL-1β	989:993	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	7	25	from	effects	965:971	arg1	IL-6					983:986	IL-6	983:986	IL-6	983:986	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	7	25	from	effects	965:971	arg1	expressions					1010:1020	COX-2 mRNA expressions	999:1020	COX-2 mRNA expressions	999:1020	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	7	25	from	effects	965:971	arg1	TNF-α					976:980	TNF-α	976:980	TNF-α	976:980	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	1	26	theme	Umbilicaria	155:165	arg1	yunnana					167:173	Umbilicaria yunnana	155:173	Umbilicaria yunnana	155:173	A polysaccharide JUYP was isolated and purified from Umbilicaria yunnana.
32084489	3	27	theme	homogeneous	396:406	arg1	JUYP					423:426	A homogeneous polysaccharide JUYP	394:426	A homogeneous polysaccharide JUYP	394:426	A homogeneous polysaccharide JUYP was obtained with the yield of 21.2% and average molecular weight (Mw) of 577 kDa.
32084489	7	28	from	IL-1β	989:993	arg1	cells					1050:1054	LPS-stimulated RAW 264.7 cells	1025:1054	LPS-stimulated RAW 264.7 cells	1025:1054	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	7	29	theme	RAW	1040:1042	arg1	cells					1050:1054	LPS-stimulated RAW 264.7 cells	1025:1054	LPS-stimulated RAW 264.7 cells	1025:1054	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	4	30	theme	2.3:1:0.7	636:644	arg1	ratio					627:631	a molar ratio	619:631	a molar ratio of 2.3:1:0.7	619:644	Monosaccharide composition analysis indicated that JUYP was composed of glucose, galactose and mannose with a molar ratio of 2.3:1:0.7.
32084489	4	31	theme	molar	621:625	arg1	ratio					627:631	a molar ratio	619:631	a molar ratio of 2.3:1:0.7	619:644	Monosaccharide composition analysis indicated that JUYP was composed of glucose, galactose and mannose with a molar ratio of 2.3:1:0.7.
32084489	3	32	theme	polysaccharide	408:421	arg1	JUYP					423:426	A homogeneous polysaccharide JUYP	394:426	A homogeneous polysaccharide JUYP	394:426	A homogeneous polysaccharide JUYP was obtained with the yield of 21.2% and average molecular weight (Mw) of 577 kDa.
32084489	6	33	theme	branched	843:850	arg1	molecule					852:859	a linear branched molecule	834:859	a linear branched molecule with entangled chains	834:881	TEM images further revealed JUYP was a linear branched molecule with entangled chains.
32084489	5	34	theme	Structural	647:656	arg1	analyses					658:665	Structural analyses	647:665	Structural analyses	647:665	Structural analyses demonstrated that the dominate components in JUYP were 6-β-D-Glcp, and other sugar residues included 2,4-β-D-Manp and T-β-D-Galf.
32084489	2	35	theme	detailed	180:187	arg1	structure					189:197	The detailed structure	176:197	The detailed structure of JUYP	176:205	The detailed structure of JUYP was studied using gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR), methylation-GC-MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM).
32084489	7	36	theme	COX-2	999:1003	arg1	expressions					1010:1020	COX-2 mRNA expressions	999:1020	COX-2 mRNA expressions	999:1020	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	3	37	theme	average	469:475	arg1	Mw					495:496	Mw	495:496	Mw	495:496	A homogeneous polysaccharide JUYP was obtained with the yield of 21.2% and average molecular weight (Mw) of 577 kDa.
32084489	3	37	theme	average	469:475	arg1	weight					487:492	average molecular weight	469:492	average molecular weight (Mw) of 577 kDa	469:508	A homogeneous polysaccharide JUYP was obtained with the yield of 21.2% and average molecular weight (Mw) of 577 kDa.
32084489	0	38	theme	Structural	43:52	arg1	characterization					54:69	Structural characterization	43:69	Structural characterization	43:69	A polysaccharide from Umbilicaria yunnana: Structural characterization and anti-inflammation effects.
32084489	4	39	theme	Monosaccharide	511:524	arg1	analysis					538:545	Monosaccharide composition analysis	511:545	Monosaccharide composition analysis	511:545	Monosaccharide composition analysis indicated that JUYP was composed of glucose, galactose and mannose with a molar ratio of 2.3:1:0.7.
32084489	7	40	theme	LPS-stimulated	1025:1038	arg1	cells					1050:1054	LPS-stimulated RAW 264.7 cells	1025:1054	LPS-stimulated RAW 264.7 cells	1025:1054	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	2	41	theme	gas	225:227	arg1	GC					245:246	GC	245:246	GC	245:246	The detailed structure of JUYP was studied using gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR), methylation-GC-MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM).
32084489	2	41	theme	gas	225:227	arg1	Fourier					250:256	Fourier	250:256	Fourier transform infrared spectroscopy (FTIR), methylation-GC-MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM)	250:391	The detailed structure of JUYP was studied using gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR), methylation-GC-MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM).
32084489	2	41	theme	gas	225:227	arg1	chromatography					229:242	gas chromatography	225:242	gas chromatography (GC)	225:247	The detailed structure of JUYP was studied using gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR), methylation-GC-MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM).
32084489	3	42	theme	molecular	477:485	arg1	Mw					495:496	Mw	495:496	Mw	495:496	A homogeneous polysaccharide JUYP was obtained with the yield of 21.2% and average molecular weight (Mw) of 577 kDa.
32084489	3	42	theme	molecular	477:485	arg1	weight					487:492	average molecular weight	469:492	average molecular weight (Mw) of 577 kDa	469:508	A homogeneous polysaccharide JUYP was obtained with the yield of 21.2% and average molecular weight (Mw) of 577 kDa.
32084489	7	43	theme	anti-inflammatory	897:913	arg1	assays					915:920	the anti-inflammatory assays	893:920	the anti-inflammatory assays	893:920	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	7	44	theme	mRNA	1005:1008	arg1	expressions					1010:1020	COX-2 mRNA expressions	999:1020	COX-2 mRNA expressions	999:1020	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	3	45	theme	%	463:463	arg1	yield					450:454	the yield	446:454	the yield of 21.2%	446:463	A homogeneous polysaccharide JUYP was obtained with the yield of 21.2% and average molecular weight (Mw) of 577 kDa.
32084489	3	45	theme	%	463:463	arg1	Mw					495:496	Mw	495:496	Mw	495:496	A homogeneous polysaccharide JUYP was obtained with the yield of 21.2% and average molecular weight (Mw) of 577 kDa.
32084489	3	45	theme	%	463:463	arg1	weight					487:492	average molecular weight	469:492	average molecular weight (Mw) of 577 kDa	469:508	A homogeneous polysaccharide JUYP was obtained with the yield of 21.2% and average molecular weight (Mw) of 577 kDa.
32084489	7	46	from	effects	1078:1084	arg1	expressions					1140:1150	IL-1β expressions	1134:1150	IL-1β expressions	1134:1150	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	2	47	theme	magnetic	325:332	arg1	NMR					345:347	NMR	345:347	NMR	345:347	The detailed structure of JUYP was studied using gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR), methylation-GC-MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM).
32084489	2	47	theme	magnetic	325:332	arg1	resonance					334:342	nuclear magnetic resonance	317:342	nuclear magnetic resonance (NMR)	317:348	The detailed structure of JUYP was studied using gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR), methylation-GC-MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM).
32084489	7	48	from	expressions	1010:1020	arg1	cells					1050:1054	LPS-stimulated RAW 264.7 cells	1025:1054	LPS-stimulated RAW 264.7 cells	1025:1054	Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
32084489	0	49	theme	anti-inflammation	75:91	arg1	effects					93:99	anti-inflammation effects	75:99	anti-inflammation effects	75:99	A polysaccharide from Umbilicaria yunnana: Structural characterization and anti-inflammation effects.
32084489	0	50	dep	polysaccharide	2:15	arg1	effects					93:99	anti-inflammation effects	75:99	anti-inflammation effects	75:99	A polysaccharide from Umbilicaria yunnana: Structural characterization and anti-inflammation effects.
32084489	0	50	dep	polysaccharide	2:15	arg1	characterization					54:69	Structural characterization	43:69	Structural characterization	43:69	A polysaccharide from Umbilicaria yunnana: Structural characterization and anti-inflammation effects.
32084489	2	51	theme	electron	367:374	arg1	TEM					388:390	TEM	388:390	TEM	388:390	The detailed structure of JUYP was studied using gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR), methylation-GC-MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM).
32084489	2	51	theme	electron	367:374	arg1	microscopy					376:385	transmission electron microscopy	354:385	transmission electron microscopy (TEM)	354:391	The detailed structure of JUYP was studied using gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR), methylation-GC-MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM).
32084489	6	52	with	molecule	852:859	arg1	chains					876:881	entangled chains	866:881	entangled chains	866:881	TEM images further revealed JUYP was a linear branched molecule with entangled chains.
32084489	5	53	theme	other	738:742	arg1	residues					750:757	other sugar residues	738:757	other sugar residues	738:757	Structural analyses demonstrated that the dominate components in JUYP were 6-β-D-Glcp, and other sugar residues included 2,4-β-D-Manp and T-β-D-Galf.
32084489	2	54	theme	transmission	354:365	arg1	TEM					388:390	TEM	388:390	TEM	388:390	The detailed structure of JUYP was studied using gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR), methylation-GC-MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM).
32084489	2	54	theme	transmission	354:365	arg1	microscopy					376:385	transmission electron microscopy	354:385	transmission electron microscopy (TEM)	354:391	The detailed structure of JUYP was studied using gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR), methylation-GC-MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM).
34119184	0	0	theme	echo	99:102	arg1	-NMR					113:116	pulsed field gradient-stimulated echo (PFG-STE)-NMR	66:116	pulsed field gradient-stimulated echo (PFG-STE)-NMR	66:116	Characterization of covalent, feruloylated polysaccharide gels by pulsed field gradient-stimulated echo (PFG-STE)-NMR.
34119184	8	1	theme	long	1123:1126	arg1	pectins					1148:1154	long time acid extracted pectins	1123:1154	long time acid extracted pectins	1123:1154	A simple correlation between rheological data and mobility of guest molecules has not generally been found: although long time acid extracted pectins form highly elastic gels, water diffusion in these gels is less hindered.
34119184	0	2	theme	gradient-stimulated	79:97	arg1	-NMR					113:116	pulsed field gradient-stimulated echo (PFG-STE)-NMR	66:116	pulsed field gradient-stimulated echo (PFG-STE)-NMR	66:116	Characterization of covalent, feruloylated polysaccharide gels by pulsed field gradient-stimulated echo (PFG-STE)-NMR.
34119184	4	3	theme	induced	540:546	arg1	coupling					548:555	Laccase induced coupling	532:555	Laccase induced coupling of these chemically characterized polymers	532:598	Laccase induced coupling of these chemically characterized polymers resulted for most of the feruloylated polysaccharides in homogeneous gels.
34119184	5	4	theme	polysaccharide	710:723	arg1	composition					725:735	polysaccharide composition	710:735	polysaccharide composition	710:735	Rheological studies confirmed that polysaccharide composition affects viscoelastic properties of the resulting gels.
34119184	8	5	theme	molecules	1074:1082	arg1	mobility					1056:1063	mobility	1056:1063	mobility of guest molecules	1056:1082	A simple correlation between rheological data and mobility of guest molecules has not generally been found: although long time acid extracted pectins form highly elastic gels, water diffusion in these gels is less hindered.
34119184	8	5	theme	molecules	1074:1082	arg1	data					1047:1050	rheological data	1035:1050	rheological data	1035:1050	A simple correlation between rheological data and mobility of guest molecules has not generally been found: although long time acid extracted pectins form highly elastic gels, water diffusion in these gels is less hindered.
34119184	2	6	theme	PFG-STE-NMR	351:361	arg1	applicability					334:346	the applicability	330:346	the applicability of PFG-STE-NMR on feruloylated gels	330:382	Here, the applicability of PFG-STE-NMR on feruloylated gels was proven, and relationships to rheological data were studied.
34119184	0	7	theme	PFG-STE	105:111	arg1	-NMR					113:116	pulsed field gradient-stimulated echo (PFG-STE)-NMR	66:116	pulsed field gradient-stimulated echo (PFG-STE)-NMR	66:116	Characterization of covalent, feruloylated polysaccharide gels by pulsed field gradient-stimulated echo (PFG-STE)-NMR.
34119184	4	8	theme	Laccase	532:538	arg1	coupling					548:555	Laccase induced coupling	532:555	Laccase induced coupling of these chemically characterized polymers	532:598	Laccase induced coupling of these chemically characterized polymers resulted for most of the feruloylated polysaccharides in homogeneous gels.
34119184	8	9	theme	acid	1133:1136	arg1	pectins					1148:1154	long time acid extracted pectins	1123:1154	long time acid extracted pectins	1123:1154	A simple correlation between rheological data and mobility of guest molecules has not generally been found: although long time acid extracted pectins form highly elastic gels, water diffusion in these gels is less hindered.
34119184	8	10	theme	guest	1068:1072	arg1	molecules					1074:1082	guest molecules	1068:1082	guest molecules	1068:1082	A simple correlation between rheological data and mobility of guest molecules has not generally been found: although long time acid extracted pectins form highly elastic gels, water diffusion in these gels is less hindered.
34119184	7	11	theme	diffusion	967:975	arg1	behavior					977:984	the diffusion behavior	963:984	the diffusion behavior of these molecules	963:1003	In the feruloylated polysaccharide gels, both polysaccharide concentration and extraction conditions affect the diffusion behavior of these molecules.
34119184	4	12	theme	feruloylated	625:636	arg1	polysaccharides					638:652	the feruloylated polysaccharides	621:652	the feruloylated polysaccharides in homogeneous gels	621:672	Laccase induced coupling of these chemically characterized polymers resulted for most of the feruloylated polysaccharides in homogeneous gels.
34119184	6	13	theme	water	816:820	arg1	diffusion					803:811	diffusion	803:811	diffusion of water and glucose	803:832	Generally, diffusion of water and glucose is hindered in gels.
34119184	8	14	theme	time	1128:1131	arg1	pectins					1148:1154	long time acid extracted pectins	1123:1154	long time acid extracted pectins	1123:1154	A simple correlation between rheological data and mobility of guest molecules has not generally been found: although long time acid extracted pectins form highly elastic gels, water diffusion in these gels is less hindered.
34119184	7	15	theme	feruloylated	862:873	arg1	gels					890:893	the feruloylated polysaccharide gels	858:893	the feruloylated polysaccharide gels	858:893	In the feruloylated polysaccharide gels, both polysaccharide concentration and extraction conditions affect the diffusion behavior of these molecules.
34119184	8	16	theme	rheological	1035:1045	arg1	data					1047:1050	rheological data	1035:1050	rheological data	1035:1050	A simple correlation between rheological data and mobility of guest molecules has not generally been found: although long time acid extracted pectins form highly elastic gels, water diffusion in these gels is less hindered.
34119184	2	17	from	applicability	334:346	arg1	gels					379:382	feruloylated gels	366:382	feruloylated gels	366:382	Here, the applicability of PFG-STE-NMR on feruloylated gels was proven, and relationships to rheological data were studied.
34119184	8	18	from	diffusion	1188:1196	arg1	gels					1207:1210	these gels	1201:1210	these gels	1201:1210	A simple correlation between rheological data and mobility of guest molecules has not generally been found: although long time acid extracted pectins form highly elastic gels, water diffusion in these gels is less hindered.
34119184	5	19	theme	Rheological	675:685	arg1	studies					687:693	Rheological studies	675:693	Rheological studies	675:693	Rheological studies confirmed that polysaccharide composition affects viscoelastic properties of the resulting gels.
34119184	4	20	from	polysaccharides	638:652	arg1	gels					669:672	homogeneous gels	657:672	homogeneous gels	657:672	Laccase induced coupling of these chemically characterized polymers resulted for most of the feruloylated polysaccharides in homogeneous gels.
34119184	7	21	theme	polysaccharide	875:888	arg1	gels					890:893	the feruloylated polysaccharide gels	858:893	the feruloylated polysaccharide gels	858:893	In the feruloylated polysaccharide gels, both polysaccharide concentration and extraction conditions affect the diffusion behavior of these molecules.
34119184	7	22	theme	extraction	934:943	arg1	conditions					945:954	both polysaccharide concentration and extraction conditions	896:954	conditions	945:954	In the feruloylated polysaccharide gels, both polysaccharide concentration and extraction conditions affect the diffusion behavior of these molecules.
34119184	4	23	theme	polymers	591:598	arg1	coupling					548:555	Laccase induced coupling	532:555	Laccase induced coupling of these chemically characterized polymers	532:598	Laccase induced coupling of these chemically characterized polymers resulted for most of the feruloylated polysaccharides in homogeneous gels.
34119184	7	24	theme	polysaccharide	901:914	arg1	concentration					916:928	both polysaccharide concentration and extraction conditions	896:954	concentration	916:928	In the feruloylated polysaccharide gels, both polysaccharide concentration and extraction conditions affect the diffusion behavior of these molecules.
34119184	4	25	theme	characterized	577:589	arg1	polymers					591:598	these chemically characterized polymers	560:598	these chemically characterized polymers	560:598	Laccase induced coupling of these chemically characterized polymers resulted for most of the feruloylated polysaccharides in homogeneous gels.
34119184	5	26	theme	resulting	776:784	arg1	gels					786:789	the resulting gels	772:789	the resulting gels	772:789	Rheological studies confirmed that polysaccharide composition affects viscoelastic properties of the resulting gels.
34119184	0	27	theme	covalent	20:27	arg1	gels					58:61	covalent, feruloylated polysaccharide gels	20:61	covalent, feruloylated polysaccharide gels	20:61	Characterization of covalent, feruloylated polysaccharide gels by pulsed field gradient-stimulated echo (PFG-STE)-NMR.
34119184	1	28	theme	nutritional	285:295	arg1	properties					312:321	their nutritional and functional properties	279:321	their nutritional and functional properties	279:321	Translational mobility of guest molecules such as water and glucose in gels that are based on feruloylated polysaccharides appears to be critical to understand their nutritional and functional properties.
34119184	5	29	theme	gels	786:789	arg1	properties					758:767	viscoelastic properties	745:767	viscoelastic properties of the resulting gels	745:789	Rheological studies confirmed that polysaccharide composition affects viscoelastic properties of the resulting gels.
34119184	2	30	theme	rheological	417:427	arg1	data					429:432	rheological data	417:432	rheological data	417:432	Here, the applicability of PFG-STE-NMR on feruloylated gels was proven, and relationships to rheological data were studied.
34119184	0	31	theme	polysaccharide	43:56	arg1	gels					58:61	covalent, feruloylated polysaccharide gels	20:61	covalent, feruloylated polysaccharide gels	20:61	Characterization of covalent, feruloylated polysaccharide gels by pulsed field gradient-stimulated echo (PFG-STE)-NMR.
34119184	3	32	theme	varying	512:518	arg1	conditions					520:529	varying conditions	512:529	varying conditions	512:529	Arabinoxylans and pectins were extracted from by-products using varying conditions.
34119184	8	33	theme	water	1182:1186	arg1	diffusion					1188:1196	water diffusion	1182:1196	water diffusion in these gels	1182:1210	A simple correlation between rheological data and mobility of guest molecules has not generally been found: although long time acid extracted pectins form highly elastic gels, water diffusion in these gels is less hindered.
34119184	8	34	theme	elastic	1168:1174	arg1	gels					1176:1179	highly elastic gels	1161:1179	highly elastic gels	1161:1179	A simple correlation between rheological data and mobility of guest molecules has not generally been found: although long time acid extracted pectins form highly elastic gels, water diffusion in these gels is less hindered.
34119184	1	35	theme	functional	301:310	arg1	properties					312:321	their nutritional and functional properties	279:321	their nutritional and functional properties	279:321	Translational mobility of guest molecules such as water and glucose in gels that are based on feruloylated polysaccharides appears to be critical to understand their nutritional and functional properties.
34119184	5	36	theme	viscoelastic	745:756	arg1	properties					758:767	viscoelastic properties	745:767	viscoelastic properties of the resulting gels	745:789	Rheological studies confirmed that polysaccharide composition affects viscoelastic properties of the resulting gels.
34119184	8	37	theme	simple	1008:1013	arg1	correlation					1015:1025	A simple correlation	1006:1025	A simple correlation between rheological data and mobility of guest molecules	1006:1082	A simple correlation between rheological data and mobility of guest molecules has not generally been found: although long time acid extracted pectins form highly elastic gels, water diffusion in these gels is less hindered.
34119184	0	38	theme	gels	58:61	arg1	Characterization					0:15	Characterization	0:15	Characterization of covalent, feruloylated polysaccharide gels by pulsed field gradient-stimulated echo (PFG-STE)-NMR	0:116	Characterization of covalent, feruloylated polysaccharide gels by pulsed field gradient-stimulated echo (PFG-STE)-NMR.
34119184	8	39	theme	extracted	1138:1146	arg1	pectins					1148:1154	long time acid extracted pectins	1123:1154	long time acid extracted pectins	1123:1154	A simple correlation between rheological data and mobility of guest molecules has not generally been found: although long time acid extracted pectins form highly elastic gels, water diffusion in these gels is less hindered.
34119184	6	40	theme	glucose	826:832	arg1	diffusion					803:811	diffusion	803:811	diffusion of water and glucose	803:832	Generally, diffusion of water and glucose is hindered in gels.
34119184	1	41	theme	Translational	119:131	arg1	mobility					133:140	Translational mobility	119:140	Translational mobility of guest molecules such as water and glucose in gels that are based on feruloylated polysaccharides	119:240	Translational mobility of guest molecules such as water and glucose in gels that are based on feruloylated polysaccharides appears to be critical to understand their nutritional and functional properties.
34119184	0	42	dep	covalent	20:27	arg1	feruloylated					30:41	feruloylated	30:41	feruloylated	30:41	Characterization of covalent, feruloylated polysaccharide gels by pulsed field gradient-stimulated echo (PFG-STE)-NMR.
34119184	1	43	from	glucose	179:185	arg1	gels					190:193	gels	190:193	gels that are based on feruloylated polysaccharides	190:240	Translational mobility of guest molecules such as water and glucose in gels that are based on feruloylated polysaccharides appears to be critical to understand their nutritional and functional properties.
34119184	1	44	theme	molecules	151:159	arg1	mobility					133:140	Translational mobility	119:140	Translational mobility of guest molecules such as water and glucose in gels that are based on feruloylated polysaccharides	119:240	Translational mobility of guest molecules such as water and glucose in gels that are based on feruloylated polysaccharides appears to be critical to understand their nutritional and functional properties.
34119184	1	45	theme	feruloylated	213:224	arg1	polysaccharides					226:240	feruloylated polysaccharides	213:240	feruloylated polysaccharides	213:240	Translational mobility of guest molecules such as water and glucose in gels that are based on feruloylated polysaccharides appears to be critical to understand their nutritional and functional properties.
34119184	0	46	theme	field	73:77	arg1	-NMR					113:116	pulsed field gradient-stimulated echo (PFG-STE)-NMR	66:116	pulsed field gradient-stimulated echo (PFG-STE)-NMR	66:116	Characterization of covalent, feruloylated polysaccharide gels by pulsed field gradient-stimulated echo (PFG-STE)-NMR.
34119184	1	47	theme	guest	145:149	arg1	glucose					179:185	glucose	179:185	glucose	179:185	Translational mobility of guest molecules such as water and glucose in gels that are based on feruloylated polysaccharides appears to be critical to understand their nutritional and functional properties.
34119184	1	47	theme	guest	145:149	arg1	molecules					151:159	guest molecules	145:159	guest molecules such as water and glucose in gels that are based on feruloylated polysaccharides	145:240	Translational mobility of guest molecules such as water and glucose in gels that are based on feruloylated polysaccharides appears to be critical to understand their nutritional and functional properties.
34119184	1	47	theme	guest	145:149	arg1	water					169:173	water	169:173	water	169:173	Translational mobility of guest molecules such as water and glucose in gels that are based on feruloylated polysaccharides appears to be critical to understand their nutritional and functional properties.
34119184	7	48	theme	molecules	995:1003	arg1	behavior					977:984	the diffusion behavior	963:984	the diffusion behavior of these molecules	963:1003	In the feruloylated polysaccharide gels, both polysaccharide concentration and extraction conditions affect the diffusion behavior of these molecules.
34119184	0	49	theme	pulsed	66:71	arg1	-NMR					113:116	pulsed field gradient-stimulated echo (PFG-STE)-NMR	66:116	pulsed field gradient-stimulated echo (PFG-STE)-NMR	66:116	Characterization of covalent, feruloylated polysaccharide gels by pulsed field gradient-stimulated echo (PFG-STE)-NMR.
34119184	1	50	from	water	169:173	arg1	gels					190:193	gels	190:193	gels that are based on feruloylated polysaccharides	190:240	Translational mobility of guest molecules such as water and glucose in gels that are based on feruloylated polysaccharides appears to be critical to understand their nutritional and functional properties.
34119184	2	51	theme	feruloylated	366:377	arg1	gels					379:382	feruloylated gels	366:382	feruloylated gels	366:382	Here, the applicability of PFG-STE-NMR on feruloylated gels was proven, and relationships to rheological data were studied.
34119184	4	52	theme	homogeneous	657:667	arg1	gels					669:672	homogeneous gels	657:672	homogeneous gels	657:672	Laccase induced coupling of these chemically characterized polymers resulted for most of the feruloylated polysaccharides in homogeneous gels.
34702475	2	0	dep	nanoparticles	339:351	arg1	films					370:374	composite films	360:374	BC/lignin nanoparticles (LNPs) composite films with retarded biodegradability, which could broaden their application area	329:449	This work prepared BC/lignin nanoparticles (LNPs) composite films with retarded biodegradability, which could broaden their application area.
34702475	1	1	theme	humid	292:296	arg1	conditions					298:307	humid conditions	292:307	humid conditions	292:307	In practical applications, the full biodegradability of all-biomass-based bacterial cellulose (BC) composites enhances their environmentally friendliness but results in the poor durability especially at humid conditions.
34702475	6	2	theme	%	995:995	arg1	degradation					997:1007	~85 wt% and ~ 97 wt% degradation	976:1007	~85 wt% and ~ 97 wt% degradation respectively	976:1020	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	6	3	theme	%	913:913	arg1	degradation					915:925	~58 wt% degradation	907:925	~58 wt% degradation	907:925	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	6	3	theme	%	913:913	arg1	property					897:904	much higher retarded degradation property	864:904	much higher retarded degradation property (~58 wt% degradation)	864:926	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	1	4	theme	composites	188:197	arg1	biodegradability					125:140	the full biodegradability	116:140	the full biodegradability of all-biomass-based bacterial cellulose (BC) composites	116:197	In practical applications, the full biodegradability of all-biomass-based bacterial cellulose (BC) composites enhances their environmentally friendliness but results in the poor durability especially at humid conditions.
34702475	6	5	dep	showed	857:862	arg1	degradation					997:1007	~85 wt% and ~ 97 wt% degradation	976:1007	~85 wt% and ~ 97 wt% degradation respectively	976:1020	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	6	6	theme	and ~ 97 wt	984:994	arg1	%					995:995	~85 wt% and ~ 97 wt%	976:995	~85 wt% and ~ 97 wt% degradation respectively	976:1020	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	7	7	theme	%	1199:1199	arg1	property					1181:1188	comparable retarded degradation property	1149:1188	comparable retarded degradation property of ~60 wt%	1149:1199	While at low enzyme loadings of 1 mg g-1 BCE, all these three composite films showed comparable retarded degradation property of ~60 wt%.
34702475	6	8	theme	~58 wt	907:912	arg1	degradation					915:925	~58 wt% degradation	907:925	~58 wt% degradation	907:925	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	6	8	theme	~58 wt	907:912	arg1	property					897:904	much higher retarded degradation property	864:904	much higher retarded degradation property (~58 wt% degradation)	864:926	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	6	9	theme	%	982:982	arg1	%					995:995	~85 wt% and ~ 97 wt%	976:995	~85 wt% and ~ 97 wt% degradation respectively	976:1020	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	2	10	theme	BC/lignin	329:337	arg1	LNPs					354:357	LNPs	354:357	LNPs	354:357	This work prepared BC/lignin nanoparticles (LNPs) composite films with retarded biodegradability, which could broaden their application area.
34702475	2	10	theme	BC/lignin	329:337	arg1	nanoparticles					339:351	BC/lignin nanoparticles	329:351	BC/lignin nanoparticles (LNPs) composite films with retarded biodegradability, which could broaden their application area	329:449	This work prepared BC/lignin nanoparticles (LNPs) composite films with retarded biodegradability, which could broaden their application area.
34702475	3	11	theme	ethanol	545:551	arg1	organosolv					553:562	ethanol organosolv	545:562	ethanol organosolv	545:562	Three LNPs were fabricated using technical lignins extracted by deep eutectic solvent (DES), ethanol organosolv, soda/anthraquinone from poplar.
34702475	3	11	theme	ethanol	545:551	arg1	solvent					530:536	deep eutectic solvent	516:536	deep eutectic solvent (DES)	516:542	Three LNPs were fabricated using technical lignins extracted by deep eutectic solvent (DES), ethanol organosolv, soda/anthraquinone from poplar.
34702475	5	12	theme	LNPs	834:837	arg1	mechanism					773:781	The potential inhibition mechanism	748:781	The potential inhibition mechanism	748:781	The potential inhibition mechanism was physical barrier and non-productive binding of LNPs.
34702475	5	12	theme	LNPs	834:837	arg1	barrier					796:802	physical barrier	787:802	physical barrier	787:802	The potential inhibition mechanism was physical barrier and non-productive binding of LNPs.
34702475	5	12	theme	LNPs	834:837	arg1	binding					823:829	non-productive binding	808:829	non-productive binding	808:829	The potential inhibition mechanism was physical barrier and non-productive binding of LNPs.
34702475	6	13	theme	~85 wt	976:981	arg1	%					995:995	~85 wt% and ~ 97 wt%	976:995	~85 wt% and ~ 97 wt% degradation respectively	976:1020	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	1	14	theme	practical	92:100	arg1	applications					102:113	practical applications	92:113	practical applications	92:113	In practical applications, the full biodegradability of all-biomass-based bacterial cellulose (BC) composites enhances their environmentally friendliness but results in the poor durability especially at humid conditions.
34702475	1	15	theme	environmentally	214:228	arg1	friendliness					230:241	their environmentally friendliness	208:241	their environmentally friendliness	208:241	In practical applications, the full biodegradability of all-biomass-based bacterial cellulose (BC) composites enhances their environmentally friendliness but results in the poor durability especially at humid conditions.
34702475	4	16	theme	films	741:745	arg1	biodegradation					713:726	the biodegradation	709:726	the biodegradation of composite films	709:745	LNPs involvement during BC fermentation showed limited influence on its productivity but significantly retarded the biodegradation of composite films.
34702475	4	17	theme	limited	644:650	arg1	influence					652:660	limited influence	644:660	limited influence	644:660	LNPs involvement during BC fermentation showed limited influence on its productivity but significantly retarded the biodegradation of composite films.
34702475	6	18	theme	degradation	885:895	arg1	degradation					915:925	~58 wt% degradation	907:925	~58 wt% degradation	907:925	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	6	18	theme	degradation	885:895	arg1	property					897:904	much higher retarded degradation property	864:904	much higher retarded degradation property (~58 wt% degradation)	864:926	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	4	19	theme	composite	731:739	arg1	films					741:745	composite films	731:745	composite films	731:745	LNPs involvement during BC fermentation showed limited influence on its productivity but significantly retarded the biodegradation of composite films.
34702475	4	20	theme	BC	621:622	arg1	fermentation					624:635	BC fermentation	621:635	BC fermentation	621:635	LNPs involvement during BC fermentation showed limited influence on its productivity but significantly retarded the biodegradation of composite films.
34702475	7	21	theme	degradation	1169:1179	arg1	property					1181:1188	comparable retarded degradation property	1149:1188	comparable retarded degradation property of ~60 wt%	1149:1199	While at low enzyme loadings of 1 mg g-1 BCE, all these three composite films showed comparable retarded degradation property of ~60 wt%.
34702475	6	22	theme	retarded	876:883	arg1	degradation					915:925	~58 wt% degradation	907:925	~58 wt% degradation	907:925	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	6	22	theme	retarded	876:883	arg1	property					897:904	much higher retarded degradation property	864:904	much higher retarded degradation property (~58 wt% degradation)	864:926	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	2	23	theme	composite	360:368	arg1	films					370:374	composite films	360:374	BC/lignin nanoparticles (LNPs) composite films with retarded biodegradability, which could broaden their application area	329:449	This work prepared BC/lignin nanoparticles (LNPs) composite films with retarded biodegradability, which could broaden their application area.
34702475	7	24	theme	composite	1126:1134	arg1	films					1136:1140	all these three composite films	1110:1140	all these three composite films	1110:1140	While at low enzyme loadings of 1 mg g-1 BCE, all these three composite films showed comparable retarded degradation property of ~60 wt%.
34702475	2	25	theme	application	434:444	arg1	area					446:449	their application area	428:449	their application area	428:449	This work prepared BC/lignin nanoparticles (LNPs) composite films with retarded biodegradability, which could broaden their application area.
34702475	0	26	theme	Bacterial	0:8	arg1	cellulose/lignin					10:25	Bacterial cellulose/lignin	0:25	Bacterial cellulose/lignin	0:25	Bacterial cellulose/lignin nanoparticles composite films with retarded biodegradability.
34702475	6	27	theme	higher	869:874	arg1	degradation					915:925	~58 wt% degradation	907:925	~58 wt% degradation	907:925	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	6	27	theme	higher	869:874	arg1	property					897:904	much higher retarded degradation property	864:904	much higher retarded degradation property (~58 wt% degradation)	864:926	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	7	28	theme	comparable	1149:1158	arg1	property					1181:1188	comparable retarded degradation property	1149:1188	comparable retarded degradation property of ~60 wt%	1149:1199	While at low enzyme loadings of 1 mg g-1 BCE, all these three composite films showed comparable retarded degradation property of ~60 wt%.
34702475	3	29	theme	technical	485:493	arg1	lignins					495:501	technical lignins	485:501	technical lignins extracted by deep eutectic solvent (DES), ethanol organosolv, soda/anthraquinone from poplar	485:594	Three LNPs were fabricated using technical lignins extracted by deep eutectic solvent (DES), ethanol organosolv, soda/anthraquinone from poplar.
34702475	0	30	theme	composite	41:49	arg1	films					51:55	composite films	41:55	composite films	41:55	Bacterial cellulose/lignin nanoparticles composite films with retarded biodegradability.
34702475	6	31	theme	5 mg g-1 BCE	1050:1061	arg1	loadings					1038:1045	high enzyme loadings	1026:1045	high enzyme loadings of 5 mg g-1 BCE	1026:1061	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	5	32	theme	physical	787:794	arg1	mechanism					773:781	The potential inhibition mechanism	748:781	The potential inhibition mechanism	748:781	The potential inhibition mechanism was physical barrier and non-productive binding of LNPs.
34702475	5	32	theme	physical	787:794	arg1	barrier					796:802	physical barrier	787:802	physical barrier	787:802	The potential inhibition mechanism was physical barrier and non-productive binding of LNPs.
34702475	1	33	theme	full	120:123	arg1	biodegradability					125:140	the full biodegradability	116:140	the full biodegradability of all-biomass-based bacterial cellulose (BC) composites	116:197	In practical applications, the full biodegradability of all-biomass-based bacterial cellulose (BC) composites enhances their environmentally friendliness but results in the poor durability especially at humid conditions.
34702475	3	34	theme	eutectic	521:528	arg1	organosolv					553:562	ethanol organosolv	545:562	ethanol organosolv	545:562	Three LNPs were fabricated using technical lignins extracted by deep eutectic solvent (DES), ethanol organosolv, soda/anthraquinone from poplar.
34702475	3	34	theme	eutectic	521:528	arg1	soda/anthraquinone					565:582	soda/anthraquinone	565:582	soda/anthraquinone	565:582	Three LNPs were fabricated using technical lignins extracted by deep eutectic solvent (DES), ethanol organosolv, soda/anthraquinone from poplar.
34702475	3	34	theme	eutectic	521:528	arg1	DES					539:541	DES	539:541	DES	539:541	Three LNPs were fabricated using technical lignins extracted by deep eutectic solvent (DES), ethanol organosolv, soda/anthraquinone from poplar.
34702475	3	34	theme	eutectic	521:528	arg1	solvent					530:536	deep eutectic solvent	516:536	deep eutectic solvent (DES)	516:542	Three LNPs were fabricated using technical lignins extracted by deep eutectic solvent (DES), ethanol organosolv, soda/anthraquinone from poplar.
34702475	7	35	theme	retarded	1160:1167	arg1	property					1181:1188	comparable retarded degradation property	1149:1188	comparable retarded degradation property of ~60 wt%	1149:1199	While at low enzyme loadings of 1 mg g-1 BCE, all these three composite films showed comparable retarded degradation property of ~60 wt%.
34702475	6	36	theme	BC/DES	963:968	arg1	LNPs					970:973	BC/DES LNPs	963:973	BC/DES LNPs	963:973	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	2	37	theme	retarded	381:388	arg1	biodegradability					390:405	retarded biodegradability	381:405	retarded biodegradability	381:405	This work prepared BC/lignin nanoparticles (LNPs) composite films with retarded biodegradability, which could broaden their application area.
34702475	2	38	with	films	370:374	arg1	biodegradability					390:405	retarded biodegradability	381:405	retarded biodegradability	381:405	This work prepared BC/lignin nanoparticles (LNPs) composite films with retarded biodegradability, which could broaden their application area.
34702475	6	39	theme	enzyme	1031:1036	arg1	loadings					1038:1045	high enzyme loadings	1026:1045	high enzyme loadings of 5 mg g-1 BCE	1026:1061	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	5	40	theme	non-productive	808:821	arg1	binding					823:829	non-productive binding	808:829	non-productive binding	808:829	The potential inhibition mechanism was physical barrier and non-productive binding of LNPs.
34702475	7	41	theme	1 mg g-1 BCE	1096:1107	arg1	loadings					1084:1091	low enzyme loadings	1073:1091	low enzyme loadings of 1 mg g-1 BCE	1073:1107	While at low enzyme loadings of 1 mg g-1 BCE, all these three composite films showed comparable retarded degradation property of ~60 wt%.
34702475	3	42	theme	deep	516:519	arg1	organosolv					553:562	ethanol organosolv	545:562	ethanol organosolv	545:562	Three LNPs were fabricated using technical lignins extracted by deep eutectic solvent (DES), ethanol organosolv, soda/anthraquinone from poplar.
34702475	3	42	theme	deep	516:519	arg1	soda/anthraquinone					565:582	soda/anthraquinone	565:582	soda/anthraquinone	565:582	Three LNPs were fabricated using technical lignins extracted by deep eutectic solvent (DES), ethanol organosolv, soda/anthraquinone from poplar.
34702475	3	42	theme	deep	516:519	arg1	DES					539:541	DES	539:541	DES	539:541	Three LNPs were fabricated using technical lignins extracted by deep eutectic solvent (DES), ethanol organosolv, soda/anthraquinone from poplar.
34702475	3	42	theme	deep	516:519	arg1	solvent					530:536	deep eutectic solvent	516:536	deep eutectic solvent (DES)	516:542	Three LNPs were fabricated using technical lignins extracted by deep eutectic solvent (DES), ethanol organosolv, soda/anthraquinone from poplar.
34702475	6	43	theme	high	1026:1029	arg1	loadings					1038:1045	high enzyme loadings	1026:1045	high enzyme loadings of 5 mg g-1 BCE	1026:1061	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	5	44	theme	potential	752:760	arg1	mechanism					773:781	The potential inhibition mechanism	748:781	The potential inhibition mechanism	748:781	The potential inhibition mechanism was physical barrier and non-productive binding of LNPs.
34702475	5	44	theme	potential	752:760	arg1	barrier					796:802	physical barrier	787:802	physical barrier	787:802	The potential inhibition mechanism was physical barrier and non-productive binding of LNPs.
34702475	6	45	theme	BC/Organosolv	940:952	arg1	LNPs					954:957	BC/Organosolv LNPs	940:957	BC/Organosolv LNPs	940:957	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	0	46	theme	retarded	62:69	arg1	biodegradability					71:86	retarded biodegradability	62:86	retarded biodegradability	62:86	Bacterial cellulose/lignin nanoparticles composite films with retarded biodegradability.
34702475	4	47	theme	LNPs	597:600	arg1	involvement					602:612	LNPs involvement	597:612	LNPs involvement during BC fermentation	597:635	LNPs involvement during BC fermentation showed limited influence on its productivity but significantly retarded the biodegradation of composite films.
34702475	5	48	theme	inhibition	762:771	arg1	mechanism					773:781	The potential inhibition mechanism	748:781	The potential inhibition mechanism	748:781	The potential inhibition mechanism was physical barrier and non-productive binding of LNPs.
34702475	5	48	theme	inhibition	762:771	arg1	barrier					796:802	physical barrier	787:802	physical barrier	787:802	The potential inhibition mechanism was physical barrier and non-productive binding of LNPs.
34702475	7	49	theme	~60 wt	1193:1198	arg1	%					1199:1199	~60 wt%	1193:1199	~60 wt%	1193:1199	While at low enzyme loadings of 1 mg g-1 BCE, all these three composite films showed comparable retarded degradation property of ~60 wt%.
34702475	6	50	theme	BC/Soda	844:850	arg1	LNPs					852:855	The BC/Soda LNPs	840:855	The BC/Soda LNPs	840:855	The BC/Soda LNPs showed much higher retarded degradation property (~58 wt% degradation) compared to BC/Organosolv LNPs and BC/DES LNPs (~85 wt% and ~ 97 wt% degradation respectively) at high enzyme loadings of 5 mg g-1 BCE.
34702475	1	51	theme	all-biomass-based	145:161	arg1	composites					188:197	all-biomass-based bacterial cellulose (BC) composites	145:197	all-biomass-based bacterial cellulose (BC) composites	145:197	In practical applications, the full biodegradability of all-biomass-based bacterial cellulose (BC) composites enhances their environmentally friendliness but results in the poor durability especially at humid conditions.
34702475	1	52	theme	bacterial	163:171	arg1	composites					188:197	all-biomass-based bacterial cellulose (BC) composites	145:197	all-biomass-based bacterial cellulose (BC) composites	145:197	In practical applications, the full biodegradability of all-biomass-based bacterial cellulose (BC) composites enhances their environmentally friendliness but results in the poor durability especially at humid conditions.
34702475	1	53	theme	poor	262:265	arg1	durability					267:276	the poor durability	258:276	the poor durability	258:276	In practical applications, the full biodegradability of all-biomass-based bacterial cellulose (BC) composites enhances their environmentally friendliness but results in the poor durability especially at humid conditions.
34702475	7	54	theme	low	1073:1075	arg1	loadings					1084:1091	low enzyme loadings	1073:1091	low enzyme loadings of 1 mg g-1 BCE	1073:1107	While at low enzyme loadings of 1 mg g-1 BCE, all these three composite films showed comparable retarded degradation property of ~60 wt%.
34702475	1	55	theme	cellulose	173:181	arg1	composites					188:197	all-biomass-based bacterial cellulose (BC) composites	145:197	all-biomass-based bacterial cellulose (BC) composites	145:197	In practical applications, the full biodegradability of all-biomass-based bacterial cellulose (BC) composites enhances their environmentally friendliness but results in the poor durability especially at humid conditions.
34702475	7	56	theme	enzyme	1077:1082	arg1	loadings					1084:1091	low enzyme loadings	1073:1091	low enzyme loadings of 1 mg g-1 BCE	1073:1107	While at low enzyme loadings of 1 mg g-1 BCE, all these three composite films showed comparable retarded degradation property of ~60 wt%.
34702475	1	57	theme	BC	184:185	arg1	composites					188:197	all-biomass-based bacterial cellulose (BC) composites	145:197	all-biomass-based bacterial cellulose (BC) composites	145:197	In practical applications, the full biodegradability of all-biomass-based bacterial cellulose (BC) composites enhances their environmentally friendliness but results in the poor durability especially at humid conditions.
33889889	10	0	theme	wall	1760:1763	arg1	damage					1765:1770	the cell wall damage	1751:1770	the cell wall damage already inflicted during Zif-8 biomineralization	1751:1819	The strongly interacting ZIF-8 biomineralized shell made encapsulated bacteria more susceptible to antibiotics, presumably due to the cell wall damage already inflicted during Zif-8 biomineralization.
33889889	11	1	theme	alginate	1893:1900	arg1	gels					1902:1905	intermediately interacting alginate gels	1866:1905	intermediately interacting alginate gels	1866:1905	Overall, weakly interacting yolk-shells and intermediately interacting alginate gels protected best and maintained probiotic activity of encapsulated bacteria.
33889889	5	2	theme	coordinate-covalent	787:805	arg1	bonding					807:813	coordinate-covalent bonding	787:813	coordinate-covalent bonding	787:813	Here, we encapsulated two probiotic strains using ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding), alginate gelation (intermediate-interaction by hydrogen bonding) or protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces).
33889889	11	3	theme	probiotic	1937:1945	arg1	activity					1947:1954	probiotic activity	1937:1954	probiotic activity of encapsulated bacteria	1937:1979	Overall, weakly interacting yolk-shells and intermediately interacting alginate gels protected best and maintained probiotic activity of encapsulated bacteria.
33889889	8	4	theme	coordinate-covalent	1358:1376	arg1	bonding					1378:1384	coordinate-covalent bonding	1358:1384	coordinate-covalent bonding of zinc and hydrogen bonding of alginate	1358:1425	Although amino groups are required for coordinate-covalent bonding of zinc and hydrogen bonding of alginate, both L. acidophilus and B. infantis could be encapsulated using ZIF-8 biomineralization and alginate gelation.
33889889	12	5	theme	different	2065:2073	arg1	aspect					2075:2080	different aspect	2065:2080	different aspect of protection described here	2065:2109	The impact of interfacial-interactions between shells and encapsulated bacteria on different aspect of protection described here, contributes to the further development of effective surface-engineered shells and its application for protecting bacteria.
33889889	10	6	theme	interacting	1634:1644	arg1	shell					1667:1671	The strongly interacting ZIF-8 biomineralized shell	1621:1671	The strongly interacting ZIF-8 biomineralized shell	1621:1671	The strongly interacting ZIF-8 biomineralized shell made encapsulated bacteria more susceptible to antibiotics, presumably due to the cell wall damage already inflicted during Zif-8 biomineralization.
33889889	2	7	theme	different	328:336	arg1	interfacial-interactions					338:361	different interfacial-interactions	328:361	different interfacial-interactions with encapsulated, bacterial surfaces	328:399	Different cell encapsulation methods exist, yielding shells with different interfacial-interactions with encapsulated, bacterial surfaces.
33889889	1	8	theme	harsh	231:235	arg1	conditions					251:260	harsh environmental conditions	231:260	harsh environmental conditions	231:260	Surface-engineered encapsulation is a non-genetic method to protect living organisms against harsh environmental conditions.
33889889	8	9	theme	bonding	1407:1413	arg1	bonding					1378:1384	coordinate-covalent bonding	1358:1384	coordinate-covalent bonding of zinc and hydrogen bonding of alginate	1358:1425	Although amino groups are required for coordinate-covalent bonding of zinc and hydrogen bonding of alginate, both L. acidophilus and B. infantis could be encapsulated using ZIF-8 biomineralization and alginate gelation.
33889889	7	10	theme	hydrophilic	1241:1251	arg1	surface					1264:1270	a hydrophilic, uncharged surface	1239:1270	a hydrophilic, uncharged surface	1239:1270	Probiotic Bifidobacterium infantis had a hydrophilic, uncharged surface, rich in polysaccharides with little proteins.
33889889	8	11	theme	alginate	1418:1425	arg1	zinc					1389:1392	zinc	1389:1392	zinc	1389:1392	Although amino groups are required for coordinate-covalent bonding of zinc and hydrogen bonding of alginate, both L. acidophilus and B. infantis could be encapsulated using ZIF-8 biomineralization and alginate gelation.
33889889	8	11	theme	alginate	1418:1425	arg1	bonding					1407:1413	hydrogen bonding	1398:1413	hydrogen bonding of alginate	1398:1425	Although amino groups are required for coordinate-covalent bonding of zinc and hydrogen bonding of alginate, both L. acidophilus and B. infantis could be encapsulated using ZIF-8 biomineralization and alginate gelation.
33889889	12	12	from	impact	1986:1991	arg1	aspect					2075:2080	different aspect	2065:2080	different aspect of protection described here	2065:2109	The impact of interfacial-interactions between shells and encapsulated bacteria on different aspect of protection described here, contributes to the further development of effective surface-engineered shells and its application for protecting bacteria.
33889889	5	13	theme	alginate	817:824	arg1	gelation					826:833	alginate gelation	817:833	alginate gelation (intermediate-interaction by hydrogen bonding)	817:880	Here, we encapsulated two probiotic strains using ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding), alginate gelation (intermediate-interaction by hydrogen bonding) or protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces).
33889889	2	14	theme	cell	273:276	arg1	methods					292:298	Different cell encapsulation methods	263:298	Different cell encapsulation methods	263:298	Different cell encapsulation methods exist, yielding shells with different interfacial-interactions with encapsulated, bacterial surfaces.
33889889	8	15	theme	ZIF-8	1492:1496	arg1	biomineralization					1498:1514	ZIF-8 biomineralization	1492:1514	ZIF-8 biomineralization	1492:1514	Although amino groups are required for coordinate-covalent bonding of zinc and hydrogen bonding of alginate, both L. acidophilus and B. infantis could be encapsulated using ZIF-8 biomineralization and alginate gelation.
33889889	7	16	theme	Bifidobacterium	1210:1224	arg1	infantis					1226:1233	Probiotic Bifidobacterium infantis	1200:1233	Probiotic Bifidobacterium infantis	1200:1233	Probiotic Bifidobacterium infantis had a hydrophilic, uncharged surface, rich in polysaccharides with little proteins.
33889889	12	17	theme	interfacial-interactions	1996:2019	arg1	impact					1986:1991	The impact	1982:1991	The impact	1982:1991	The impact of interfacial-interactions between shells and encapsulated bacteria on different aspect of protection described here, contributes to the further development of effective surface-engineered shells and its application for protecting bacteria.
33889889	5	18	theme	SiO2	915:918	arg1	nanoparticles					920:932	SiO2 nanoparticles	915:932	SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces)	915:1025	Here, we encapsulated two probiotic strains using ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding), alginate gelation (intermediate-interaction by hydrogen bonding) or protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces).
33889889	3	19	with	bacteria	524:531	arg1	composition					556:566	different surface composition	538:566	different surface composition	538:566	However, the impact of interfacial-interactions on the protection offered by different shells is unclear and can vary for bacteria with different surface composition.
33889889	2	20	dep	encapsulated	368:379	arg1	bacterial					382:390	bacterial	382:390	bacterial	382:390	Different cell encapsulation methods exist, yielding shells with different interfacial-interactions with encapsulated, bacterial surfaces.
33889889	6	21	theme	negatively-charged	1163:1180	arg1	one					1182:1184	and a negatively-charged one	1157:1184	one	1182:1184	The surface of probiotic Lactobacillus acidophilus was rich in protein, yielding a hydrophilic, positively-charged surface below and a negatively-charged one above pH 4.0.
33889889	5	22	dep	gelation	826:833	arg1	intermediate-interaction					836:859	intermediate-interaction	836:859	intermediate-interaction	836:859	Here, we encapsulated two probiotic strains using ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding), alginate gelation (intermediate-interaction by hydrogen bonding) or protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces).
33889889	4	23	theme	gastro-intestinal	615:631	arg1	fluids					633:638	gastro-intestinal fluids	615:638	gastro-intestinal fluids	615:638	Probiotic bacteria require protection against gastro-intestinal fluids and antibiotics.
33889889	10	24	theme	biomineralized	1652:1665	arg1	shell					1667:1671	The strongly interacting ZIF-8 biomineralized shell	1621:1671	The strongly interacting ZIF-8 biomineralized shell	1621:1671	The strongly interacting ZIF-8 biomineralized shell made encapsulated bacteria more susceptible to antibiotics, presumably due to the cell wall damage already inflicted during Zif-8 biomineralization.
33889889	0	25	theme	cell	112:115	arg1	composition					125:135	different cell surface composition	102:135	different cell surface composition	102:135	Interfacial interactions between protective, surface-engineered shells and encapsulated bacteria with different cell surface composition.
33889889	5	26	theme	imidazolate	723:733	arg1	ZIF-8					707:711	ZIF-8	707:711	ZIF-8	707:711	Here, we encapsulated two probiotic strains using ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding), alginate gelation (intermediate-interaction by hydrogen bonding) or protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces).
33889889	5	26	theme	imidazolate	723:733	arg1	framework					735:743	zeolitic imidazolate framework	714:743	zeolitic imidazolate framework	714:743	Here, we encapsulated two probiotic strains using ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding), alginate gelation (intermediate-interaction by hydrogen bonding) or protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces).
33889889	9	27	dep	shells	1587:1592	arg1	all					1594:1596	all	1594:1596	all	1594:1596	Weakly, intermediately and strongly interacting shells all yielded porous shells.
33889889	5	28	theme	probiotic	683:691	arg1	strains					693:699	two probiotic strains	679:699	two probiotic strains using ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding), alginate gelation (intermediate-interaction by hydrogen bonding) or protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces)	679:1025	Here, we encapsulated two probiotic strains using ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding), alginate gelation (intermediate-interaction by hydrogen bonding) or protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces).
33889889	0	29	theme	Interfacial	0:10	arg1	interactions					12:23	Interfacial interactions	0:23	Interfacial interactions between protective, surface-engineered shells and encapsulated bacteria with different cell surface composition	0:135	Interfacial interactions between protective, surface-engineered shells and encapsulated bacteria with different cell surface composition.
33889889	12	30	theme	protecting	2214:2223	arg1	bacteria					2225:2232	protecting bacteria	2214:2232	protecting bacteria	2214:2232	The impact of interfacial-interactions between shells and encapsulated bacteria on different aspect of protection described here, contributes to the further development of effective surface-engineered shells and its application for protecting bacteria.
33889889	3	31	theme	surface	548:554	arg1	composition					556:566	different surface composition	538:566	different surface composition	538:566	However, the impact of interfacial-interactions on the protection offered by different shells is unclear and can vary for bacteria with different surface composition.
33889889	12	32	theme	surface-engineered	2164:2181	arg1	shells					2183:2188	effective surface-engineered shells	2154:2188	effective surface-engineered shells	2154:2188	The impact of interfacial-interactions between shells and encapsulated bacteria on different aspect of protection described here, contributes to the further development of effective surface-engineered shells and its application for protecting bacteria.
33889889	0	33	theme	encapsulated	75:86	arg1	bacteria					88:95	encapsulated bacteria	75:95	encapsulated bacteria	75:95	Interfacial interactions between protective, surface-engineered shells and encapsulated bacteria with different cell surface composition.
33889889	10	34	theme	cell	1755:1758	arg1	damage					1765:1770	the cell wall damage	1751:1770	the cell wall damage already inflicted during Zif-8 biomineralization	1751:1819	The strongly interacting ZIF-8 biomineralized shell made encapsulated bacteria more susceptible to antibiotics, presumably due to the cell wall damage already inflicted during Zif-8 biomineralization.
33889889	11	35	theme	encapsulated	1959:1970	arg1	bacteria					1972:1979	encapsulated bacteria	1959:1979	encapsulated bacteria	1959:1979	Overall, weakly interacting yolk-shells and intermediately interacting alginate gels protected best and maintained probiotic activity of encapsulated bacteria.
33889889	11	36	theme	interacting	1838:1848	arg1	yolk-shells					1850:1860	weakly interacting yolk-shells	1831:1860	weakly interacting yolk-shells	1831:1860	Overall, weakly interacting yolk-shells and intermediately interacting alginate gels protected best and maintained probiotic activity of encapsulated bacteria.
33889889	9	37	theme	interacting	1575:1585	arg1	shells					1587:1592	intermediately and strongly interacting shells	1547:1592	intermediately and strongly interacting shells all	1547:1596	Weakly, intermediately and strongly interacting shells all yielded porous shells.
33889889	12	38	theme	further	2131:2137	arg1	development					2139:2149	the further development	2127:2149	the further development of effective surface-engineered shells	2127:2188	The impact of interfacial-interactions between shells and encapsulated bacteria on different aspect of protection described here, contributes to the further development of effective surface-engineered shells and its application for protecting bacteria.
33889889	12	39	theme	protection	2085:2094	arg1	aspect					2075:2080	different aspect	2065:2080	different aspect of protection described here	2065:2109	The impact of interfacial-interactions between shells and encapsulated bacteria on different aspect of protection described here, contributes to the further development of effective surface-engineered shells and its application for protecting bacteria.
33889889	6	40	from	rich	1083:1086	arg1	protein					1091:1097	protein	1091:1097	protein	1091:1097	The surface of probiotic Lactobacillus acidophilus was rich in protein, yielding a hydrophilic, positively-charged surface below and a negatively-charged one above pH 4.0.
33889889	11	41	theme	interacting	1881:1891	arg1	gels					1902:1905	intermediately interacting alginate gels	1866:1905	intermediately interacting alginate gels	1866:1905	Overall, weakly interacting yolk-shells and intermediately interacting alginate gels protected best and maintained probiotic activity of encapsulated bacteria.
33889889	9	42	theme	porous	1606:1611	arg1	shells					1613:1618	porous shells	1606:1618	porous shells	1606:1618	Weakly, intermediately and strongly interacting shells all yielded porous shells.
33889889	6	43	from	protein	1091:1097	arg1	rich					1083:1086	rich	1083:1086	rich	1083:1086	The surface of probiotic Lactobacillus acidophilus was rich in protein, yielding a hydrophilic, positively-charged surface below and a negatively-charged one above pH 4.0.
33889889	6	43	from	protein	1091:1097	arg1	surface					1032:1038	The surface	1028:1038	The surface of probiotic Lactobacillus acidophilus	1028:1077	The surface of probiotic Lactobacillus acidophilus was rich in protein, yielding a hydrophilic, positively-charged surface below and a negatively-charged one above pH 4.0.
33889889	5	44	theme	nanoparticles	920:932	arg1	gelation					826:833	alginate gelation	817:833	alginate gelation (intermediate-interaction by hydrogen bonding)	817:880	Here, we encapsulated two probiotic strains using ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding), alginate gelation (intermediate-interaction by hydrogen bonding) or protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces).
33889889	5	44	theme	nanoparticles	920:932	arg1	packing					904:910	protamine-assisted packing	885:910	protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces)	885:1025	Here, we encapsulated two probiotic strains using ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding), alginate gelation (intermediate-interaction by hydrogen bonding) or protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces).
33889889	5	44	theme	nanoparticles	920:932	arg1	biomineralization					746:762	ZIF-8 (zeolitic imidazolate framework) biomineralization	707:762	ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding)	707:814	Here, we encapsulated two probiotic strains using ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding), alginate gelation (intermediate-interaction by hydrogen bonding) or protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces).
33889889	7	45	theme	little	1302:1307	arg1	proteins					1309:1316	little proteins	1302:1316	little proteins	1302:1316	Probiotic Bifidobacterium infantis had a hydrophilic, uncharged surface, rich in polysaccharides with little proteins.
33889889	8	46	theme	amino	1328:1332	arg1	groups					1334:1339	amino groups	1328:1339	amino groups	1328:1339	Although amino groups are required for coordinate-covalent bonding of zinc and hydrogen bonding of alginate, both L. acidophilus and B. infantis could be encapsulated using ZIF-8 biomineralization and alginate gelation.
33889889	2	47	theme	encapsulated	368:379	arg1	surfaces					392:399	encapsulated, bacterial surfaces	368:399	encapsulated, bacterial surfaces	368:399	Different cell encapsulation methods exist, yielding shells with different interfacial-interactions with encapsulated, bacterial surfaces.
33889889	6	48	theme	acidophilus	1067:1077	arg1	rich					1083:1086	rich	1083:1086	rich	1083:1086	The surface of probiotic Lactobacillus acidophilus was rich in protein, yielding a hydrophilic, positively-charged surface below and a negatively-charged one above pH 4.0.
33889889	6	48	theme	acidophilus	1067:1077	arg1	surface					1032:1038	The surface	1028:1038	The surface of probiotic Lactobacillus acidophilus	1028:1077	The surface of probiotic Lactobacillus acidophilus was rich in protein, yielding a hydrophilic, positively-charged surface below and a negatively-charged one above pH 4.0.
33889889	10	49	theme	Zif-8	1797:1801	arg1	biomineralization					1803:1819	Zif-8 biomineralization	1797:1819	Zif-8 biomineralization	1797:1819	The strongly interacting ZIF-8 biomineralized shell made encapsulated bacteria more susceptible to antibiotics, presumably due to the cell wall damage already inflicted during Zif-8 biomineralization.
33889889	7	50	theme	rich	1273:1276	arg1	surface					1264:1270	a hydrophilic, uncharged surface	1239:1270	a hydrophilic, uncharged surface	1239:1270	Probiotic Bifidobacterium infantis had a hydrophilic, uncharged surface, rich in polysaccharides with little proteins.
33889889	1	51	theme	living	206:211	arg1	organisms					213:221	living organisms	206:221	living organisms	206:221	Surface-engineered encapsulation is a non-genetic method to protect living organisms against harsh environmental conditions.
33889889	5	52	dep	yolk-shell	945:954	arg1	weak-interaction					957:972	weak-interaction	957:972	weak-interaction across a void between shells and bacterial surfaces	957:1024	Here, we encapsulated two probiotic strains using ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding), alginate gelation (intermediate-interaction by hydrogen bonding) or protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces).
33889889	12	53	theme	encapsulated	2040:2051	arg1	bacteria					2053:2060	encapsulated bacteria	2040:2060	encapsulated bacteria	2040:2060	The impact of interfacial-interactions between shells and encapsulated bacteria on different aspect of protection described here, contributes to the further development of effective surface-engineered shells and its application for protecting bacteria.
33889889	1	54	theme	environmental	237:249	arg1	conditions					251:260	harsh environmental conditions	231:260	harsh environmental conditions	231:260	Surface-engineered encapsulation is a non-genetic method to protect living organisms against harsh environmental conditions.
33889889	8	55	theme	zinc	1389:1392	arg1	bonding					1378:1384	coordinate-covalent bonding	1358:1384	coordinate-covalent bonding of zinc and hydrogen bonding of alginate	1358:1425	Although amino groups are required for coordinate-covalent bonding of zinc and hydrogen bonding of alginate, both L. acidophilus and B. infantis could be encapsulated using ZIF-8 biomineralization and alginate gelation.
33889889	6	56	theme	positively-charged	1124:1141	arg1	surface					1143:1149	a hydrophilic, positively-charged surface	1109:1149	surface	1143:1149	The surface of probiotic Lactobacillus acidophilus was rich in protein, yielding a hydrophilic, positively-charged surface below and a negatively-charged one above pH 4.0.
33889889	5	57	theme	hydrogen	864:871	arg1	bonding					873:879	hydrogen bonding	864:879	hydrogen bonding	864:879	Here, we encapsulated two probiotic strains using ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding), alginate gelation (intermediate-interaction by hydrogen bonding) or protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces).
33889889	5	58	theme	bacterial	1007:1015	arg1	surfaces					1017:1024	bacterial surfaces	1007:1024	bacterial surfaces	1007:1024	Here, we encapsulated two probiotic strains using ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding), alginate gelation (intermediate-interaction by hydrogen bonding) or protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces).
33889889	8	59	theme	hydrogen	1398:1405	arg1	bonding					1407:1413	hydrogen bonding	1398:1413	hydrogen bonding of alginate	1398:1425	Although amino groups are required for coordinate-covalent bonding of zinc and hydrogen bonding of alginate, both L. acidophilus and B. infantis could be encapsulated using ZIF-8 biomineralization and alginate gelation.
33889889	6	60	theme	hydrophilic	1111:1121	arg1	surface					1143:1149	a hydrophilic, positively-charged surface	1109:1149	surface	1143:1149	The surface of probiotic Lactobacillus acidophilus was rich in protein, yielding a hydrophilic, positively-charged surface below and a negatively-charged one above pH 4.0.
33889889	2	61	theme	encapsulation	278:290	arg1	methods					292:298	Different cell encapsulation methods	263:298	Different cell encapsulation methods	263:298	Different cell encapsulation methods exist, yielding shells with different interfacial-interactions with encapsulated, bacterial surfaces.
33889889	1	62	theme	Surface-engineered	138:155	arg1	method					188:193	a non-genetic method	174:193	a non-genetic method to protect living organisms against harsh environmental conditions	174:260	Surface-engineered encapsulation is a non-genetic method to protect living organisms against harsh environmental conditions.
33889889	1	62	theme	Surface-engineered	138:155	arg1	encapsulation					157:169	Surface-engineered encapsulation	138:169	Surface-engineered encapsulation	138:169	Surface-engineered encapsulation is a non-genetic method to protect living organisms against harsh environmental conditions.
33889889	7	63	theme	Probiotic	1200:1208	arg1	infantis					1226:1233	Probiotic Bifidobacterium infantis	1200:1233	Probiotic Bifidobacterium infantis	1200:1233	Probiotic Bifidobacterium infantis had a hydrophilic, uncharged surface, rich in polysaccharides with little proteins.
33889889	2	64	with	interfacial-interactions	338:361	arg1	surfaces					392:399	encapsulated, bacterial surfaces	368:399	encapsulated, bacterial surfaces	368:399	Different cell encapsulation methods exist, yielding shells with different interfacial-interactions with encapsulated, bacterial surfaces.
33889889	3	65	from	impact	415:420	arg1	protection					457:466	the protection	453:466	the protection offered by different shells	453:494	However, the impact of interfacial-interactions on the protection offered by different shells is unclear and can vary for bacteria with different surface composition.
33889889	1	66	theme	non-genetic	176:186	arg1	method					188:193	a non-genetic method	174:193	a non-genetic method to protect living organisms against harsh environmental conditions	174:260	Surface-engineered encapsulation is a non-genetic method to protect living organisms against harsh environmental conditions.
33889889	1	66	theme	non-genetic	176:186	arg1	encapsulation					157:169	Surface-engineered encapsulation	138:169	Surface-engineered encapsulation	138:169	Surface-engineered encapsulation is a non-genetic method to protect living organisms against harsh environmental conditions.
33889889	0	67	theme	different	102:110	arg1	composition					125:135	different cell surface composition	102:135	different cell surface composition	102:135	Interfacial interactions between protective, surface-engineered shells and encapsulated bacteria with different cell surface composition.
33889889	8	68	theme	alginate	1520:1527	arg1	gelation					1529:1536	alginate gelation	1520:1536	alginate gelation	1520:1536	Although amino groups are required for coordinate-covalent bonding of zinc and hydrogen bonding of alginate, both L. acidophilus and B. infantis could be encapsulated using ZIF-8 biomineralization and alginate gelation.
33889889	0	69	theme	surface	117:123	arg1	composition					125:135	different cell surface composition	102:135	different cell surface composition	102:135	Interfacial interactions between protective, surface-engineered shells and encapsulated bacteria with different cell surface composition.
33889889	5	70	theme	zeolitic	714:721	arg1	ZIF-8					707:711	ZIF-8	707:711	ZIF-8	707:711	Here, we encapsulated two probiotic strains using ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding), alginate gelation (intermediate-interaction by hydrogen bonding) or protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces).
33889889	5	70	theme	zeolitic	714:721	arg1	framework					735:743	zeolitic imidazolate framework	714:743	zeolitic imidazolate framework	714:743	Here, we encapsulated two probiotic strains using ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding), alginate gelation (intermediate-interaction by hydrogen bonding) or protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces).
33889889	4	71	theme	Probiotic	569:577	arg1	bacteria					579:586	Probiotic bacteria	569:586	Probiotic bacteria	569:586	Probiotic bacteria require protection against gastro-intestinal fluids and antibiotics.
33889889	10	72	theme	ZIF-8	1646:1650	arg1	shell					1667:1671	The strongly interacting ZIF-8 biomineralized shell	1621:1671	The strongly interacting ZIF-8 biomineralized shell	1621:1671	The strongly interacting ZIF-8 biomineralized shell made encapsulated bacteria more susceptible to antibiotics, presumably due to the cell wall damage already inflicted during Zif-8 biomineralization.
33889889	5	73	theme	protamine-assisted	885:902	arg1	packing					904:910	protamine-assisted packing	885:910	protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces)	885:1025	Here, we encapsulated two probiotic strains using ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding), alginate gelation (intermediate-interaction by hydrogen bonding) or protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces).
33889889	3	74	theme	interfacial-interactions	425:448	arg1	impact					415:420	the impact	411:420	the impact of interfacial-interactions on the protection offered by different shells	411:494	However, the impact of interfacial-interactions on the protection offered by different shells is unclear and can vary for bacteria with different surface composition.
33889889	3	74	theme	interfacial-interactions	425:448	arg1	unclear					499:505	unclear	499:505	unclear	499:505	However, the impact of interfacial-interactions on the protection offered by different shells is unclear and can vary for bacteria with different surface composition.
33889889	7	75	contain	had	1235:1237	arg1	infantis					1226:1233	Probiotic Bifidobacterium infantis	1200:1233	Probiotic Bifidobacterium infantis	1200:1233	Probiotic Bifidobacterium infantis had a hydrophilic, uncharged surface, rich in polysaccharides with little proteins.
33889889	7	75	contain	had	1235:1237	arg2	surface					1264:1270	a hydrophilic, uncharged surface	1239:1270	a hydrophilic, uncharged surface	1239:1270	Probiotic Bifidobacterium infantis had a hydrophilic, uncharged surface, rich in polysaccharides with little proteins.
33889889	3	76	theme	different	479:487	arg1	shells					489:494	different shells	479:494	different shells	479:494	However, the impact of interfacial-interactions on the protection offered by different shells is unclear and can vary for bacteria with different surface composition.
33889889	10	77	theme	encapsulated	1678:1689	arg1	bacteria					1691:1698	encapsulated bacteria	1678:1698	encapsulated bacteria	1678:1698	The strongly interacting ZIF-8 biomineralized shell made encapsulated bacteria more susceptible to antibiotics, presumably due to the cell wall damage already inflicted during Zif-8 biomineralization.
33889889	0	78	theme	protective	33:42	arg1	shells					64:69	protective, surface-engineered shells	33:69	protective, surface-engineered shells	33:69	Interfacial interactions between protective, surface-engineered shells and encapsulated bacteria with different cell surface composition.
33889889	12	79	theme	shells	2183:2188	arg1	application					2198:2208	its application	2194:2208	its application for protecting bacteria	2194:2232	The impact of interfacial-interactions between shells and encapsulated bacteria on different aspect of protection described here, contributes to the further development of effective surface-engineered shells and its application for protecting bacteria.
33889889	12	79	theme	shells	2183:2188	arg1	development					2139:2149	the further development	2127:2149	the further development of effective surface-engineered shells	2127:2188	The impact of interfacial-interactions between shells and encapsulated bacteria on different aspect of protection described here, contributes to the further development of effective surface-engineered shells and its application for protecting bacteria.
33889889	5	80	theme	ZIF-8	707:711	arg1	biomineralization					746:762	ZIF-8 (zeolitic imidazolate framework) biomineralization	707:762	ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding)	707:814	Here, we encapsulated two probiotic strains using ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding), alginate gelation (intermediate-interaction by hydrogen bonding) or protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces).
33889889	7	81	from	rich	1273:1276	arg1	polysaccharides					1281:1295	polysaccharides	1281:1295	polysaccharides with little proteins	1281:1316	Probiotic Bifidobacterium infantis had a hydrophilic, uncharged surface, rich in polysaccharides with little proteins.
33889889	11	82	theme	bacteria	1972:1979	arg1	activity					1947:1954	probiotic activity	1937:1954	probiotic activity of encapsulated bacteria	1937:1979	Overall, weakly interacting yolk-shells and intermediately interacting alginate gels protected best and maintained probiotic activity of encapsulated bacteria.
33889889	7	83	from	polysaccharides	1281:1295	arg1	rich					1273:1276	rich	1273:1276	rich	1273:1276	Probiotic Bifidobacterium infantis had a hydrophilic, uncharged surface, rich in polysaccharides with little proteins.
33889889	3	84	theme	different	538:546	arg1	composition					556:566	different surface composition	538:566	different surface composition	538:566	However, the impact of interfacial-interactions on the protection offered by different shells is unclear and can vary for bacteria with different surface composition.
33889889	5	85	dep	biomineralization	746:762	arg1	strong-interaction					765:782	strong-interaction	765:782	strong-interaction	765:782	Here, we encapsulated two probiotic strains using ZIF-8 (zeolitic imidazolate framework) biomineralization (strong-interaction by coordinate-covalent bonding), alginate gelation (intermediate-interaction by hydrogen bonding) or protamine-assisted packing of SiO2 nanoparticles yielding a yolk-shell (weak-interaction across a void between shells and bacterial surfaces).
33889889	2	86	theme	Different	263:271	arg1	methods					292:298	Different cell encapsulation methods	263:298	Different cell encapsulation methods	263:298	Different cell encapsulation methods exist, yielding shells with different interfacial-interactions with encapsulated, bacterial surfaces.
33889889	0	87	with	interactions	12:23	arg1	composition					125:135	different cell surface composition	102:135	different cell surface composition	102:135	Interfacial interactions between protective, surface-engineered shells and encapsulated bacteria with different cell surface composition.
33889889	12	88	theme	effective	2154:2162	arg1	shells					2183:2188	effective surface-engineered shells	2154:2188	effective surface-engineered shells	2154:2188	The impact of interfacial-interactions between shells and encapsulated bacteria on different aspect of protection described here, contributes to the further development of effective surface-engineered shells and its application for protecting bacteria.
33889889	0	89	dep	protective	33:42	arg1	surface-engineered					45:62	surface-engineered	45:62	surface-engineered	45:62	Interfacial interactions between protective, surface-engineered shells and encapsulated bacteria with different cell surface composition.
33889889	7	90	with	polysaccharides	1281:1295	arg1	proteins					1309:1316	little proteins	1302:1316	little proteins	1302:1316	Probiotic Bifidobacterium infantis had a hydrophilic, uncharged surface, rich in polysaccharides with little proteins.
33889889	7	91	dep	hydrophilic	1241:1251	arg1	uncharged					1254:1262	uncharged	1254:1262	uncharged	1254:1262	Probiotic Bifidobacterium infantis had a hydrophilic, uncharged surface, rich in polysaccharides with little proteins.
34294317	5	0	theme	peak	927:930	arg1	rate					945:948	peak heat release rate	927:948	peak heat release rate	927:948	Results indicated that peak heat release rate and total heat release of SCC-B decreased from 30 W/g to 20 W/g and 15 kJ/g to 10 kJ/g, respectively.
34294317	4	1	dep	Flynn-Wall-Ozawa	865:880	arg1	models					896:901	models	896:901	models	896:901	The thermal kinetic behavior of SCC-B was investigated by Flynn-Wall-Ozawa and Kissinger models.
34294317	7	2	theme	clothing	1267:1274	arg1	fields					1241:1246	the fields	1237:1246	the fields of fire-protection clothing	1237:1274	These characteristics combine to suggest hopeful prospects for use of SCC-B in the fields of fire-protection clothing as a renewable flame-retardant material.
34294317	2	3	theme	sodium	377:382	arg1	aerogels					457:464	lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels	354:464	lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B)	354:472	Herein, we report an eco-friendly strategy to construct lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B) by freeze-drying and post-cross-linking technology.
34294317	2	3	theme	sodium	377:382	arg1	SCC-B					467:471	SCC-B	467:471	SCC-B	467:471	Herein, we report an eco-friendly strategy to construct lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B) by freeze-drying and post-cross-linking technology.
34294317	1	4	theme	excellent	257:265	arg1	properties					286:295	excellent thermal insulation properties	257:295	excellent thermal insulation properties	257:295	Bio-based aerogel (polysaccharide cryogel) have led to a growing interest because of eco-friendliness, sustainability and excellent thermal insulation properties.
34294317	5	5	theme	heat	932:935	arg1	rate					945:948	peak heat release rate	927:948	peak heat release rate	927:948	Results indicated that peak heat release rate and total heat release of SCC-B decreased from 30 W/g to 20 W/g and 15 kJ/g to 10 kJ/g, respectively.
34294317	2	6	theme	porous	370:375	arg1	aerogels					457:464	lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels	354:464	lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B)	354:472	Herein, we report an eco-friendly strategy to construct lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B) by freeze-drying and post-cross-linking technology.
34294317	2	6	theme	porous	370:375	arg1	SCC-B					467:471	SCC-B	467:471	SCC-B	467:471	Herein, we report an eco-friendly strategy to construct lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B) by freeze-drying and post-cross-linking technology.
34294317	1	7	theme	thermal	267:273	arg1	properties					286:295	excellent thermal insulation properties	257:295	excellent thermal insulation properties	257:295	Bio-based aerogel (polysaccharide cryogel) have led to a growing interest because of eco-friendliness, sustainability and excellent thermal insulation properties.
34294317	1	8	theme	insulation	275:284	arg1	properties					286:295	excellent thermal insulation properties	257:295	excellent thermal insulation properties	257:295	Bio-based aerogel (polysaccharide cryogel) have led to a growing interest because of eco-friendliness, sustainability and excellent thermal insulation properties.
34294317	0	9	theme	thermal	102:108	arg1	kinetics					110:117	thermal kinetics	102:117	thermal kinetics	102:117	Ecofriendly flame-retardant composite aerogel derived from polysaccharide: Preparation, flammability, thermal kinetics, and mechanism.
34294317	7	10	theme	fire-protection	1251:1265	arg1	clothing					1267:1274	fire-protection clothing	1251:1274	fire-protection clothing	1251:1274	These characteristics combine to suggest hopeful prospects for use of SCC-B in the fields of fire-protection clothing as a renewable flame-retardant material.
34294317	3	11	theme	web-like	594:601	arg1	structure					613:621	strong web-like entangled structure	587:621	strong web-like entangled structure	587:621	The ester cross-linking of polysaccharide component achieved strong web-like entangled structure when using 1,2,3,4-butanetetracarboxylic acid and sodium hypophosphite as eco-friendly co-additives, meanwhile significantly improved flame retardancy of SCC-B due to phosphorylation.
34294317	1	12	theme	properties	286:295	arg1	interest					200:207	a growing interest	190:207	a growing interest because of eco-friendliness, sustainability and excellent thermal insulation properties	190:295	Bio-based aerogel (polysaccharide cryogel) have led to a growing interest because of eco-friendliness, sustainability and excellent thermal insulation properties.
34294317	4	13	theme	SCC-B	839:843	arg1	behavior					827:834	The thermal kinetic behavior	807:834	The thermal kinetic behavior of SCC-B	807:843	The thermal kinetic behavior of SCC-B was investigated by Flynn-Wall-Ozawa and Kissinger models.
34294317	5	14	theme	heat	960:963	arg1	release					965:971	total heat release	954:971	total heat release of SCC-B	954:980	Results indicated that peak heat release rate and total heat release of SCC-B decreased from 30 W/g to 20 W/g and 15 kJ/g to 10 kJ/g, respectively.
34294317	4	15	theme	kinetic	819:825	arg1	behavior					827:834	The thermal kinetic behavior	807:834	The thermal kinetic behavior of SCC-B	807:843	The thermal kinetic behavior of SCC-B was investigated by Flynn-Wall-Ozawa and Kissinger models.
34294317	7	16	theme	renewable	1281:1289	arg1	material					1307:1314	a renewable flame-retardant material	1279:1314	a renewable flame-retardant material	1279:1314	These characteristics combine to suggest hopeful prospects for use of SCC-B in the fields of fire-protection clothing as a renewable flame-retardant material.
34294317	7	16	theme	renewable	1281:1289	arg1	prospects					1207:1215	hopeful prospects	1199:1215	hopeful prospects for use of SCC-B in the fields of fire-protection clothing	1199:1274	These characteristics combine to suggest hopeful prospects for use of SCC-B in the fields of fire-protection clothing as a renewable flame-retardant material.
34294317	7	17	theme	flame-retardant	1291:1305	arg1	material					1307:1314	a renewable flame-retardant material	1279:1314	a renewable flame-retardant material	1279:1314	These characteristics combine to suggest hopeful prospects for use of SCC-B in the fields of fire-protection clothing as a renewable flame-retardant material.
34294317	7	17	theme	flame-retardant	1291:1305	arg1	prospects					1207:1215	hopeful prospects	1199:1215	hopeful prospects for use of SCC-B in the fields of fire-protection clothing	1199:1274	These characteristics combine to suggest hopeful prospects for use of SCC-B in the fields of fire-protection clothing as a renewable flame-retardant material.
34294317	3	18	theme	1,2,3,4-butanetetracarboxylic	634:662	arg1	acid					664:667	1,2,3,4-butanetetracarboxylic acid	634:667	1,2,3,4-butanetetracarboxylic acid	634:667	The ester cross-linking of polysaccharide component achieved strong web-like entangled structure when using 1,2,3,4-butanetetracarboxylic acid and sodium hypophosphite as eco-friendly co-additives, meanwhile significantly improved flame retardancy of SCC-B due to phosphorylation.
34294317	3	18	theme	1,2,3,4-butanetetracarboxylic	634:662	arg1	hypophosphite					680:692	sodium hypophosphite	673:692	sodium hypophosphite	673:692	The ester cross-linking of polysaccharide component achieved strong web-like entangled structure when using 1,2,3,4-butanetetracarboxylic acid and sodium hypophosphite as eco-friendly co-additives, meanwhile significantly improved flame retardancy of SCC-B due to phosphorylation.
34294317	3	18	theme	1,2,3,4-butanetetracarboxylic	634:662	arg1	co-additives					710:721	eco-friendly co-additives	697:721	eco-friendly co-additives	697:721	The ester cross-linking of polysaccharide component achieved strong web-like entangled structure when using 1,2,3,4-butanetetracarboxylic acid and sodium hypophosphite as eco-friendly co-additives, meanwhile significantly improved flame retardancy of SCC-B due to phosphorylation.
34294317	0	19	theme	flame-retardant	12:26	arg1	aerogel					38:44	Ecofriendly flame-retardant composite aerogel	0:44	Ecofriendly flame-retardant composite aerogel	0:44	Ecofriendly flame-retardant composite aerogel derived from polysaccharide: Preparation, flammability, thermal kinetics, and mechanism.
34294317	7	20	theme	SCC-B	1228:1232	arg1	use					1221:1223	use	1221:1223	use of SCC-B	1221:1232	These characteristics combine to suggest hopeful prospects for use of SCC-B in the fields of fire-protection clothing as a renewable flame-retardant material.
34294317	2	21	theme	composite	447:455	arg1	aerogels					457:464	lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels	354:464	lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B)	354:472	Herein, we report an eco-friendly strategy to construct lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B) by freeze-drying and post-cross-linking technology.
34294317	2	21	theme	composite	447:455	arg1	SCC-B					467:471	SCC-B	467:471	SCC-B	467:471	Herein, we report an eco-friendly strategy to construct lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B) by freeze-drying and post-cross-linking technology.
34294317	0	22	theme	Ecofriendly	0:10	arg1	aerogel					38:44	Ecofriendly flame-retardant composite aerogel	0:44	Ecofriendly flame-retardant composite aerogel	0:44	Ecofriendly flame-retardant composite aerogel derived from polysaccharide: Preparation, flammability, thermal kinetics, and mechanism.
34294317	2	23	theme	eco-friendly	319:330	arg1	strategy					332:339	an eco-friendly strategy	316:339	an eco-friendly strategy to construct lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B) by freeze-drying and post-cross-linking technology	316:523	Herein, we report an eco-friendly strategy to construct lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B) by freeze-drying and post-cross-linking technology.
34294317	2	24	theme	polysaccharide-based	426:445	arg1	aerogels					457:464	lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels	354:464	lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B)	354:472	Herein, we report an eco-friendly strategy to construct lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B) by freeze-drying and post-cross-linking technology.
34294317	2	24	theme	polysaccharide-based	426:445	arg1	SCC-B					467:471	SCC-B	467:471	SCC-B	467:471	Herein, we report an eco-friendly strategy to construct lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B) by freeze-drying and post-cross-linking technology.
34294317	2	25	theme	post-cross-linking	495:512	arg1	technology					514:523	freeze-drying and post-cross-linking technology	477:523	freeze-drying and post-cross-linking technology	477:523	Herein, we report an eco-friendly strategy to construct lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B) by freeze-drying and post-cross-linking technology.
34294317	3	26	theme	sodium	673:678	arg1	acid					664:667	1,2,3,4-butanetetracarboxylic acid	634:667	1,2,3,4-butanetetracarboxylic acid	634:667	The ester cross-linking of polysaccharide component achieved strong web-like entangled structure when using 1,2,3,4-butanetetracarboxylic acid and sodium hypophosphite as eco-friendly co-additives, meanwhile significantly improved flame retardancy of SCC-B due to phosphorylation.
34294317	3	26	theme	sodium	673:678	arg1	hypophosphite					680:692	sodium hypophosphite	673:692	sodium hypophosphite	673:692	The ester cross-linking of polysaccharide component achieved strong web-like entangled structure when using 1,2,3,4-butanetetracarboxylic acid and sodium hypophosphite as eco-friendly co-additives, meanwhile significantly improved flame retardancy of SCC-B due to phosphorylation.
34294317	3	26	theme	sodium	673:678	arg1	co-additives					710:721	eco-friendly co-additives	697:721	eco-friendly co-additives	697:721	The ester cross-linking of polysaccharide component achieved strong web-like entangled structure when using 1,2,3,4-butanetetracarboxylic acid and sodium hypophosphite as eco-friendly co-additives, meanwhile significantly improved flame retardancy of SCC-B due to phosphorylation.
34294317	2	27	theme	freeze-drying	477:489	arg1	technology					514:523	freeze-drying and post-cross-linking technology	477:523	freeze-drying and post-cross-linking technology	477:523	Herein, we report an eco-friendly strategy to construct lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B) by freeze-drying and post-cross-linking technology.
34294317	0	28	theme	composite	28:36	arg1	aerogel					38:44	Ecofriendly flame-retardant composite aerogel	0:44	Ecofriendly flame-retardant composite aerogel	0:44	Ecofriendly flame-retardant composite aerogel derived from polysaccharide: Preparation, flammability, thermal kinetics, and mechanism.
34294317	3	29	theme	entangled	603:611	arg1	structure					613:621	strong web-like entangled structure	587:621	strong web-like entangled structure	587:621	The ester cross-linking of polysaccharide component achieved strong web-like entangled structure when using 1,2,3,4-butanetetracarboxylic acid and sodium hypophosphite as eco-friendly co-additives, meanwhile significantly improved flame retardancy of SCC-B due to phosphorylation.
34294317	5	30	theme	release	937:943	arg1	rate					945:948	peak heat release rate	927:948	peak heat release rate	927:948	Results indicated that peak heat release rate and total heat release of SCC-B decreased from 30 W/g to 20 W/g and 15 kJ/g to 10 kJ/g, respectively.
34294317	3	31	theme	strong	587:592	arg1	structure					613:621	strong web-like entangled structure	587:621	strong web-like entangled structure	587:621	The ester cross-linking of polysaccharide component achieved strong web-like entangled structure when using 1,2,3,4-butanetetracarboxylic acid and sodium hypophosphite as eco-friendly co-additives, meanwhile significantly improved flame retardancy of SCC-B due to phosphorylation.
34294317	4	32	theme	thermal	811:817	arg1	behavior					827:834	The thermal kinetic behavior	807:834	The thermal kinetic behavior of SCC-B	807:843	The thermal kinetic behavior of SCC-B was investigated by Flynn-Wall-Ozawa and Kissinger models.
34294317	3	33	theme	eco-friendly	697:708	arg1	acid					664:667	1,2,3,4-butanetetracarboxylic acid	634:667	1,2,3,4-butanetetracarboxylic acid	634:667	The ester cross-linking of polysaccharide component achieved strong web-like entangled structure when using 1,2,3,4-butanetetracarboxylic acid and sodium hypophosphite as eco-friendly co-additives, meanwhile significantly improved flame retardancy of SCC-B due to phosphorylation.
34294317	3	33	theme	eco-friendly	697:708	arg1	hypophosphite					680:692	sodium hypophosphite	673:692	sodium hypophosphite	673:692	The ester cross-linking of polysaccharide component achieved strong web-like entangled structure when using 1,2,3,4-butanetetracarboxylic acid and sodium hypophosphite as eco-friendly co-additives, meanwhile significantly improved flame retardancy of SCC-B due to phosphorylation.
34294317	3	33	theme	eco-friendly	697:708	arg1	co-additives					710:721	eco-friendly co-additives	697:721	eco-friendly co-additives	697:721	The ester cross-linking of polysaccharide component achieved strong web-like entangled structure when using 1,2,3,4-butanetetracarboxylic acid and sodium hypophosphite as eco-friendly co-additives, meanwhile significantly improved flame retardancy of SCC-B due to phosphorylation.
34294317	6	34	theme	SCC-B	1096:1100	arg1	time					1088:1091	the second-degree burn time	1065:1091	the second-degree burn time of SCC-B	1065:1100	Furthermore, the second-degree burn time of SCC-B reached up to 87.1 s under heat exposure of 11.3 kW/m2.
34294317	3	35	theme	SCC-B	777:781	arg1	retardancy					763:772	flame retardancy	757:772	flame retardancy of SCC-B due to phosphorylation	757:804	The ester cross-linking of polysaccharide component achieved strong web-like entangled structure when using 1,2,3,4-butanetetracarboxylic acid and sodium hypophosphite as eco-friendly co-additives, meanwhile significantly improved flame retardancy of SCC-B due to phosphorylation.
34294317	3	36	theme	ester	530:534	arg1	cross-linking					536:548	The ester cross-linking	526:548	The ester cross-linking of polysaccharide component	526:576	The ester cross-linking of polysaccharide component achieved strong web-like entangled structure when using 1,2,3,4-butanetetracarboxylic acid and sodium hypophosphite as eco-friendly co-additives, meanwhile significantly improved flame retardancy of SCC-B due to phosphorylation.
34294317	5	37	dep	20 W/g	1007:1012	arg1	30 W/g					997:1002	30 W/g	997:1002	30 W/g	997:1002	Results indicated that peak heat release rate and total heat release of SCC-B decreased from 30 W/g to 20 W/g and 15 kJ/g to 10 kJ/g, respectively.
34294317	7	38	from	prospects	1207:1215	arg1	fields					1241:1246	the fields	1237:1246	the fields of fire-protection clothing	1237:1274	These characteristics combine to suggest hopeful prospects for use of SCC-B in the fields of fire-protection clothing as a renewable flame-retardant material.
34294317	5	39	theme	total	954:958	arg1	release					965:971	total heat release	954:971	total heat release of SCC-B	954:980	Results indicated that peak heat release rate and total heat release of SCC-B decreased from 30 W/g to 20 W/g and 15 kJ/g to 10 kJ/g, respectively.
34294317	7	40	theme	hopeful	1199:1205	arg1	material					1307:1314	a renewable flame-retardant material	1279:1314	a renewable flame-retardant material	1279:1314	These characteristics combine to suggest hopeful prospects for use of SCC-B in the fields of fire-protection clothing as a renewable flame-retardant material.
34294317	7	40	theme	hopeful	1199:1205	arg1	prospects					1207:1215	hopeful prospects	1199:1215	hopeful prospects for use of SCC-B in the fields of fire-protection clothing	1199:1274	These characteristics combine to suggest hopeful prospects for use of SCC-B in the fields of fire-protection clothing as a renewable flame-retardant material.
34294317	0	41	dep	derived	46:52	arg1	flammability					88:99	flammability	88:99	flammability	88:99	Ecofriendly flame-retardant composite aerogel derived from polysaccharide: Preparation, flammability, thermal kinetics, and mechanism.
34294317	0	41	dep	derived	46:52	arg1	kinetics					110:117	thermal kinetics	102:117	thermal kinetics	102:117	Ecofriendly flame-retardant composite aerogel derived from polysaccharide: Preparation, flammability, thermal kinetics, and mechanism.
34294317	0	41	dep	derived	46:52	arg1	mechanism					124:132	mechanism	124:132	mechanism	124:132	Ecofriendly flame-retardant composite aerogel derived from polysaccharide: Preparation, flammability, thermal kinetics, and mechanism.
34294317	0	41	dep	derived	46:52	arg1	Preparation					75:85	Preparation	75:85	Preparation	75:85	Ecofriendly flame-retardant composite aerogel derived from polysaccharide: Preparation, flammability, thermal kinetics, and mechanism.
34294317	2	42	theme	lightweight	354:364	arg1	aerogels					457:464	lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels	354:464	lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B)	354:472	Herein, we report an eco-friendly strategy to construct lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B) by freeze-drying and post-cross-linking technology.
34294317	2	42	theme	lightweight	354:364	arg1	SCC-B					467:471	SCC-B	467:471	SCC-B	467:471	Herein, we report an eco-friendly strategy to construct lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B) by freeze-drying and post-cross-linking technology.
34294317	6	43	theme	burn	1083:1086	arg1	time					1088:1091	the second-degree burn time	1065:1091	the second-degree burn time of SCC-B	1065:1100	Furthermore, the second-degree burn time of SCC-B reached up to 87.1 s under heat exposure of 11.3 kW/m2.
34294317	1	44	theme	Bio-based	135:143	arg1	aerogel					145:151	Bio-based aerogel	135:151	Bio-based aerogel (polysaccharide cryogel)	135:176	Bio-based aerogel (polysaccharide cryogel) have led to a growing interest because of eco-friendliness, sustainability and excellent thermal insulation properties.
34294317	1	44	theme	Bio-based	135:143	arg1	cryogel					169:175	polysaccharide cryogel	154:175	polysaccharide cryogel	154:175	Bio-based aerogel (polysaccharide cryogel) have led to a growing interest because of eco-friendliness, sustainability and excellent thermal insulation properties.
34294317	1	45	theme	eco-friendliness	220:235	arg1	interest					200:207	a growing interest	190:207	a growing interest because of eco-friendliness, sustainability and excellent thermal insulation properties	190:295	Bio-based aerogel (polysaccharide cryogel) have led to a growing interest because of eco-friendliness, sustainability and excellent thermal insulation properties.
34294317	5	46	theme	SCC-B	976:980	arg1	release					965:971	total heat release	954:971	total heat release of SCC-B	954:980	Results indicated that peak heat release rate and total heat release of SCC-B decreased from 30 W/g to 20 W/g and 15 kJ/g to 10 kJ/g, respectively.
34294317	5	46	theme	SCC-B	976:980	arg1	rate					945:948	peak heat release rate	927:948	peak heat release rate	927:948	Results indicated that peak heat release rate and total heat release of SCC-B decreased from 30 W/g to 20 W/g and 15 kJ/g to 10 kJ/g, respectively.
34294317	1	47	theme	polysaccharide	154:167	arg1	aerogel					145:151	Bio-based aerogel	135:151	Bio-based aerogel (polysaccharide cryogel)	135:176	Bio-based aerogel (polysaccharide cryogel) have led to a growing interest because of eco-friendliness, sustainability and excellent thermal insulation properties.
34294317	1	47	theme	polysaccharide	154:167	arg1	cryogel					169:175	polysaccharide cryogel	154:175	polysaccharide cryogel	154:175	Bio-based aerogel (polysaccharide cryogel) have led to a growing interest because of eco-friendliness, sustainability and excellent thermal insulation properties.
34294317	3	48	theme	polysaccharide	553:566	arg1	component					568:576	polysaccharide component	553:576	polysaccharide component	553:576	The ester cross-linking of polysaccharide component achieved strong web-like entangled structure when using 1,2,3,4-butanetetracarboxylic acid and sodium hypophosphite as eco-friendly co-additives, meanwhile significantly improved flame retardancy of SCC-B due to phosphorylation.
34294317	1	49	theme	growing	192:198	arg1	interest					200:207	a growing interest	190:207	a growing interest because of eco-friendliness, sustainability and excellent thermal insulation properties	190:295	Bio-based aerogel (polysaccharide cryogel) have led to a growing interest because of eco-friendliness, sustainability and excellent thermal insulation properties.
34294317	2	50	theme	cellulose/chitosan	407:424	arg1	aerogels					457:464	lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels	354:464	lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B)	354:472	Herein, we report an eco-friendly strategy to construct lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B) by freeze-drying and post-cross-linking technology.
34294317	2	50	theme	cellulose/chitosan	407:424	arg1	SCC-B					467:471	SCC-B	467:471	SCC-B	467:471	Herein, we report an eco-friendly strategy to construct lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B) by freeze-drying and post-cross-linking technology.
34294317	3	51	theme	component	568:576	arg1	cross-linking					536:548	The ester cross-linking	526:548	The ester cross-linking of polysaccharide component	526:576	The ester cross-linking of polysaccharide component achieved strong web-like entangled structure when using 1,2,3,4-butanetetracarboxylic acid and sodium hypophosphite as eco-friendly co-additives, meanwhile significantly improved flame retardancy of SCC-B due to phosphorylation.
34294317	1	52	theme	sustainability	238:251	arg1	interest					200:207	a growing interest	190:207	a growing interest because of eco-friendliness, sustainability and excellent thermal insulation properties	190:295	Bio-based aerogel (polysaccharide cryogel) have led to a growing interest because of eco-friendliness, sustainability and excellent thermal insulation properties.
34294317	6	53	theme	heat	1129:1132	arg1	exposure					1134:1141	heat exposure	1129:1141	heat exposure of 11.3 kW/m2	1129:1155	Furthermore, the second-degree burn time of SCC-B reached up to 87.1 s under heat exposure of 11.3 kW/m2.
34294317	6	54	theme	second-degree	1069:1081	arg1	burn					1083:1086	second-degree burn	1069:1086	the second-degree burn time of SCC-B	1065:1100	Furthermore, the second-degree burn time of SCC-B reached up to 87.1 s under heat exposure of 11.3 kW/m2.
34294317	3	55	theme	flame	757:761	arg1	retardancy					763:772	flame retardancy	757:772	flame retardancy of SCC-B due to phosphorylation	757:804	The ester cross-linking of polysaccharide component achieved strong web-like entangled structure when using 1,2,3,4-butanetetracarboxylic acid and sodium hypophosphite as eco-friendly co-additives, meanwhile significantly improved flame retardancy of SCC-B due to phosphorylation.
34294317	2	56	theme	alginate/carboxymethyl	384:405	arg1	aerogels					457:464	lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels	354:464	lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B)	354:472	Herein, we report an eco-friendly strategy to construct lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B) by freeze-drying and post-cross-linking technology.
34294317	2	56	theme	alginate/carboxymethyl	384:405	arg1	SCC-B					467:471	SCC-B	467:471	SCC-B	467:471	Herein, we report an eco-friendly strategy to construct lightweight and porous sodium alginate/carboxymethyl cellulose/chitosan polysaccharide-based composite aerogels (SCC-B) by freeze-drying and post-cross-linking technology.
34294317	6	57	theme	11.3 kW/m2	1146:1155	arg1	exposure					1134:1141	heat exposure	1129:1141	heat exposure of 11.3 kW/m2	1129:1155	Furthermore, the second-degree burn time of SCC-B reached up to 87.1 s under heat exposure of 11.3 kW/m2.
32230810	0	0	theme	Plant-Derived	93:105	arg1	Gallotannins					107:118	Plant-Derived Gallotannins	93:118	Plant-Derived Gallotannins	93:118	Enhancement of Biomimetic Enzymatic Mineralization of Gellan Gum Polysaccharide Hydrogels by Plant-Derived Gallotannins.
32230810	7	1	theme	osteoblast-like	967:981	arg1	cells					990:994	Human osteoblast-like Saos-2 cells	961:994	Human osteoblast-like Saos-2 cells	961:994	Human osteoblast-like Saos-2 cells grew in eluate from mineralized hydrogels.
32230810	4	2	theme	Mangifera	682:690	arg1	L					699:699	Mangifera indica L.	682:700	Mangifera indica L.	682:700	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	4	2	theme	Mangifera	682:690	arg1	kernel					674:679	mango kernel	668:679	mango kernel (Mangifera indica L.)	668:701	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	6	3	theme	gallotannin	806:816	arg1	preparations					818:829	The various gallotannin preparations	794:829	The various gallotannin preparations	794:829	The various gallotannin preparations bound differently to ALP and influenced the size of aggregates of ALP, which may be related to ability to promote mineralization.
32230810	2	4	theme	plant-derived	370:382	arg1	polyphenols					384:394	plant-derived polyphenols	370:394	plant-derived polyphenols	370:394	Mineralization can be both induced by the enzyme alkaline phosphatase (ALP) and promoted by calcium-binding biomolecules, such as plant-derived polyphenols.
32230810	4	5	from	kernel	674:679	arg1	MW					599:600	MW	599:600	MW	599:600	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	4	5	from	kernel	674:679	arg1	PGG					626:628	PGG	626:628	PGG	626:628	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	4	5	from	kernel	674:679	arg1	acids					562:566	namely three tannic acids	542:566	namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.)	542:701	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	4	5	from	kernel	674:679	arg1	glucose					617:623	pentagalloyl glucose	604:623	pentagalloyl glucose (PGG)	604:629	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	4	5	from	kernel	674:679	arg1	weight					591:596	differing molecular weight	571:596	differing molecular weight (MW)	571:601	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	4	5	from	kernel	674:679	arg1	extract					655:661	a gallotannin-rich extract	636:661	a gallotannin-rich extract from mango kernel (Mangifera indica L.)	636:701	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	4	6	theme	weight	591:596	arg1	acids					562:566	namely three tannic acids	542:566	namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.)	542:701	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	6	7	theme	ALP	897:899	arg1	aggregates					883:892	aggregates	883:892	aggregates of ALP	883:899	The various gallotannin preparations bound differently to ALP and influenced the size of aggregates of ALP, which may be related to ability to promote mineralization.
32230810	6	8	theme	various	798:804	arg1	preparations					818:829	The various gallotannin preparations	794:829	The various gallotannin preparations	794:829	The various gallotannin preparations bound differently to ALP and influenced the size of aggregates of ALP, which may be related to ability to promote mineralization.
32230810	5	9	theme	Certain	704:710	arg1	preparations					724:735	Certain gallotannin preparations	704:735	Certain gallotannin preparations	704:735	Certain gallotannin preparations promoted mineralization to a greater degree than others.
32230810	5	10	theme	gallotannin	712:722	arg1	preparations					724:735	Certain gallotannin preparations	704:735	Certain gallotannin preparations	704:735	Certain gallotannin preparations promoted mineralization to a greater degree than others.
32230810	1	11	theme	hydrogel	139:146	arg1	biomaterials					148:159	hydrogel biomaterials	139:159	hydrogel biomaterials	139:159	Mineralization of hydrogel biomaterials with calcium phosphate (CaP) is considered advantageous for bone regeneration.
32230810	8	12	theme	impeded	1065:1071	arg1	growth					1078:1083	Gallotannin incorporation impeded cell growth	1039:1083	Gallotannin incorporation impeded cell growth on hydrogels	1039:1096	Gallotannin incorporation impeded cell growth on hydrogels and did not impart antibacterial activity.
32230810	4	13	theme	gallotannin-rich	638:653	arg1	extract					655:661	a gallotannin-rich extract	636:661	a gallotannin-rich extract from mango kernel (Mangifera indica L.)	636:701	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	0	14	theme	Gellan	54:59	arg1	Hydrogels					80:88	Gellan Gum Polysaccharide Hydrogels	54:88	Gellan Gum Polysaccharide Hydrogels	54:88	Enhancement of Biomimetic Enzymatic Mineralization of Gellan Gum Polysaccharide Hydrogels by Plant-Derived Gallotannins.
32230810	1	15	with	Mineralization	121:134	arg1	CaP					185:187	CaP	185:187	CaP	185:187	Mineralization of hydrogel biomaterials with calcium phosphate (CaP) is considered advantageous for bone regeneration.
32230810	1	15	with	Mineralization	121:134	arg1	phosphate					174:182	calcium phosphate	166:182	calcium phosphate (CaP)	166:188	Mineralization of hydrogel biomaterials with calcium phosphate (CaP) is considered advantageous for bone regeneration.
32230810	4	16	theme	differing	571:579	arg1	MW					599:600	MW	599:600	MW	599:600	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	4	16	theme	differing	571:579	arg1	weight					591:596	differing molecular weight	571:596	differing molecular weight (MW)	571:601	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	6	17	theme	aggregates	883:892	arg1	size					875:878	the size	871:878	the size	871:878	The various gallotannin preparations bound differently to ALP and influenced the size of aggregates of ALP, which may be related to ability to promote mineralization.
32230810	6	17	theme	aggregates	883:892	arg1	related					915:921	related	915:921	related	915:921	The various gallotannin preparations bound differently to ALP and influenced the size of aggregates of ALP, which may be related to ability to promote mineralization.
32230810	2	18	theme	calcium-binding	332:346	arg1	polyphenols					384:394	plant-derived polyphenols	370:394	plant-derived polyphenols	370:394	Mineralization can be both induced by the enzyme alkaline phosphatase (ALP) and promoted by calcium-binding biomolecules, such as plant-derived polyphenols.
32230810	2	18	theme	calcium-binding	332:346	arg1	biomolecules					348:359	calcium-binding biomolecules	332:359	calcium-binding biomolecules	332:359	Mineralization can be both induced by the enzyme alkaline phosphatase (ALP) and promoted by calcium-binding biomolecules, such as plant-derived polyphenols.
32230810	2	19	link	plant-derived	370:382	arg1	polyphenols					384:394	plant-derived polyphenols	370:394	plant-derived polyphenols	370:394	Mineralization can be both induced by the enzyme alkaline phosphatase (ALP) and promoted by calcium-binding biomolecules, such as plant-derived polyphenols.
32230810	3	20	theme	ALP-loaded	412:421	arg1	gum					430:432	ALP-loaded gellan gum	412:432	ALP-loaded gellan gum (GG) hydrogels	412:447	In this study, ALP-loaded gellan gum (GG) hydrogels were enriched with gallotannins, a subclass of polyphenols.
32230810	3	20	theme	ALP-loaded	412:421	arg1	GG					435:436	GG	435:436	GG	435:436	In this study, ALP-loaded gellan gum (GG) hydrogels were enriched with gallotannins, a subclass of polyphenols.
32230810	8	21	theme	cell	1073:1076	arg1	growth					1078:1083	Gallotannin incorporation impeded cell growth	1039:1083	Gallotannin incorporation impeded cell growth on hydrogels	1039:1096	Gallotannin incorporation impeded cell growth on hydrogels and did not impart antibacterial activity.
32230810	4	22	theme	extract	655:661	arg1	acids					562:566	namely three tannic acids	542:566	namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.)	542:701	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	0	23	theme	Enzymatic	26:34	arg1	Mineralization					36:49	Biomimetic Enzymatic Mineralization	15:49	Biomimetic Enzymatic Mineralization of Gellan Gum Polysaccharide Hydrogels	15:88	Enhancement of Biomimetic Enzymatic Mineralization of Gellan Gum Polysaccharide Hydrogels by Plant-Derived Gallotannins.
32230810	9	24	theme	gallotannin	1156:1166	arg1	incorporation					1168:1180	gallotannin incorporation	1156:1180	gallotannin incorporation	1156:1180	In conclusion, gallotannin incorporation aided mineralization but reduced cytocompatibility.
32230810	1	25	theme	bone	221:224	arg1	regeneration					226:237	bone regeneration	221:237	bone regeneration	221:237	Mineralization of hydrogel biomaterials with calcium phosphate (CaP) is considered advantageous for bone regeneration.
32230810	0	26	theme	Biomimetic	15:24	arg1	Mineralization					36:49	Biomimetic Enzymatic Mineralization	15:49	Biomimetic Enzymatic Mineralization of Gellan Gum Polysaccharide Hydrogels	15:88	Enhancement of Biomimetic Enzymatic Mineralization of Gellan Gum Polysaccharide Hydrogels by Plant-Derived Gallotannins.
32230810	4	27	theme	molecular	581:589	arg1	MW					599:600	MW	599:600	MW	599:600	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	4	27	theme	molecular	581:589	arg1	weight					591:596	differing molecular weight	571:596	differing molecular weight (MW)	571:601	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	5	28	theme	greater	766:772	arg1	degree					774:779	a greater degree	764:779	a greater degree than others	764:791	Certain gallotannin preparations promoted mineralization to a greater degree than others.
32230810	4	29	theme	indica	692:697	arg1	L					699:699	Mangifera indica L.	682:700	Mangifera indica L.	682:700	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	4	29	theme	indica	692:697	arg1	kernel					674:679	mango kernel	668:679	mango kernel (Mangifera indica L.)	668:701	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	0	30	theme	Mineralization	36:49	arg1	Enhancement					0:10	Enhancement	0:10	Enhancement of Biomimetic Enzymatic Mineralization of Gellan Gum Polysaccharide Hydrogels by Plant-Derived Gallotannins.	0:119	Enhancement of Biomimetic Enzymatic Mineralization of Gellan Gum Polysaccharide Hydrogels by Plant-Derived Gallotannins.
32230810	8	31	from	growth	1078:1083	arg1	hydrogels					1088:1096	hydrogels	1088:1096	hydrogels	1088:1096	Gallotannin incorporation impeded cell growth on hydrogels and did not impart antibacterial activity.
32230810	3	32	theme	gellan	423:428	arg1	gum					430:432	ALP-loaded gellan gum	412:432	ALP-loaded gellan gum (GG) hydrogels	412:447	In this study, ALP-loaded gellan gum (GG) hydrogels were enriched with gallotannins, a subclass of polyphenols.
32230810	3	32	theme	gellan	423:428	arg1	GG					435:436	GG	435:436	GG	435:436	In this study, ALP-loaded gellan gum (GG) hydrogels were enriched with gallotannins, a subclass of polyphenols.
32230810	0	33	theme	Polysaccharide	65:78	arg1	Hydrogels					80:88	Gellan Gum Polysaccharide Hydrogels	54:88	Gellan Gum Polysaccharide Hydrogels	54:88	Enhancement of Biomimetic Enzymatic Mineralization of Gellan Gum Polysaccharide Hydrogels by Plant-Derived Gallotannins.
32230810	3	34	theme	polyphenols	496:506	arg1	gallotannins					468:479	gallotannins	468:479	gallotannins	468:479	In this study, ALP-loaded gellan gum (GG) hydrogels were enriched with gallotannins, a subclass of polyphenols.
32230810	3	34	theme	polyphenols	496:506	arg1	subclass					484:491	a subclass	482:491	a subclass of polyphenols	482:506	In this study, ALP-loaded gellan gum (GG) hydrogels were enriched with gallotannins, a subclass of polyphenols.
32230810	3	35	theme	gum	430:432	arg1	hydrogels					439:447	ALP-loaded gellan gum (GG) hydrogels	412:447	ALP-loaded gellan gum (GG) hydrogels	412:447	In this study, ALP-loaded gellan gum (GG) hydrogels were enriched with gallotannins, a subclass of polyphenols.
32230810	4	36	theme	tannic	555:560	arg1	acids					562:566	namely three tannic acids	542:566	namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.)	542:701	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	1	37	theme	biomaterials	148:159	arg1	Mineralization					121:134	Mineralization	121:134	Mineralization of hydrogel biomaterials with calcium phosphate (CaP)	121:188	Mineralization of hydrogel biomaterials with calcium phosphate (CaP) is considered advantageous for bone regeneration.
32230810	7	38	theme	mineralized	1016:1026	arg1	hydrogels					1028:1036	mineralized hydrogels	1016:1036	mineralized hydrogels	1016:1036	Human osteoblast-like Saos-2 cells grew in eluate from mineralized hydrogels.
32230810	2	39	theme	alkaline	289:296	arg1	ALP					311:313	ALP	311:313	ALP	311:313	Mineralization can be both induced by the enzyme alkaline phosphatase (ALP) and promoted by calcium-binding biomolecules, such as plant-derived polyphenols.
32230810	2	39	theme	alkaline	289:296	arg1	phosphatase					298:308	enzyme alkaline phosphatase	282:308	the enzyme alkaline phosphatase (ALP)	278:314	Mineralization can be both induced by the enzyme alkaline phosphatase (ALP) and promoted by calcium-binding biomolecules, such as plant-derived polyphenols.
32230810	4	40	theme	pentagalloyl	604:615	arg1	PGG					626:628	PGG	626:628	PGG	626:628	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	4	40	theme	pentagalloyl	604:615	arg1	glucose					617:623	pentagalloyl glucose	604:623	pentagalloyl glucose (PGG)	604:629	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	8	41	theme	antibacterial	1117:1129	arg1	activity					1131:1138	antibacterial activity	1117:1138	antibacterial activity	1117:1138	Gallotannin incorporation impeded cell growth on hydrogels and did not impart antibacterial activity.
32230810	2	42	theme	enzyme	282:287	arg1	ALP					311:313	ALP	311:313	ALP	311:313	Mineralization can be both induced by the enzyme alkaline phosphatase (ALP) and promoted by calcium-binding biomolecules, such as plant-derived polyphenols.
32230810	2	42	theme	enzyme	282:287	arg1	phosphatase					298:308	enzyme alkaline phosphatase	282:308	the enzyme alkaline phosphatase (ALP)	278:314	Mineralization can be both induced by the enzyme alkaline phosphatase (ALP) and promoted by calcium-binding biomolecules, such as plant-derived polyphenols.
32230810	7	43	theme	Saos-2	983:988	arg1	cells					990:994	Human osteoblast-like Saos-2 cells	961:994	Human osteoblast-like Saos-2 cells	961:994	Human osteoblast-like Saos-2 cells grew in eluate from mineralized hydrogels.
32230810	1	44	theme	calcium	166:172	arg1	CaP					185:187	CaP	185:187	CaP	185:187	Mineralization of hydrogel biomaterials with calcium phosphate (CaP) is considered advantageous for bone regeneration.
32230810	1	44	theme	calcium	166:172	arg1	phosphate					174:182	calcium phosphate	166:182	calcium phosphate (CaP)	166:188	Mineralization of hydrogel biomaterials with calcium phosphate (CaP) is considered advantageous for bone regeneration.
32230810	7	45	from	hydrogels	1028:1036	arg1	eluate					1004:1009	eluate	1004:1009	eluate from mineralized hydrogels	1004:1036	Human osteoblast-like Saos-2 cells grew in eluate from mineralized hydrogels.
32230810	0	46	theme	Hydrogels	80:88	arg1	Mineralization					36:49	Biomimetic Enzymatic Mineralization	15:49	Biomimetic Enzymatic Mineralization of Gellan Gum Polysaccharide Hydrogels	15:88	Enhancement of Biomimetic Enzymatic Mineralization of Gellan Gum Polysaccharide Hydrogels by Plant-Derived Gallotannins.
32230810	0	47	theme	Gum	61:63	arg1	Hydrogels					80:88	Gellan Gum Polysaccharide Hydrogels	54:88	Gellan Gum Polysaccharide Hydrogels	54:88	Enhancement of Biomimetic Enzymatic Mineralization of Gellan Gum Polysaccharide Hydrogels by Plant-Derived Gallotannins.
32230810	4	48	from	acids	562:566	arg1	L					699:699	Mangifera indica L.	682:700	Mangifera indica L.	682:700	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	4	48	from	acids	562:566	arg1	kernel					674:679	mango kernel	668:679	mango kernel (Mangifera indica L.)	668:701	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	7	49	theme	Human	961:965	arg1	cells					990:994	Human osteoblast-like Saos-2 cells	961:994	Human osteoblast-like Saos-2 cells	961:994	Human osteoblast-like Saos-2 cells grew in eluate from mineralized hydrogels.
32230810	4	50	theme	mango	668:672	arg1	L					699:699	Mangifera indica L.	682:700	Mangifera indica L.	682:700	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	4	50	theme	mango	668:672	arg1	kernel					674:679	mango kernel	668:679	mango kernel (Mangifera indica L.)	668:701	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
32230810	4	51	theme	glucose	617:623	arg1	acids					562:566	namely three tannic acids	542:566	namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.)	542:701	Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.).
33183601	0	0	theme	dextran	73:79	arg1	sulfate					81:87	dextran sulfate	73:87	dextran sulfate sodium-induced acute bowel inflammation	73:127	Composites of yeast glucan particles and curcumin lead to improvement of dextran sulfate sodium-induced acute bowel inflammation in rats.
33183601	1	1	theme	composite	302:310	arg1	effect					274:279	the beneficial effect	259:279	the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats	259:379	The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats.
33183601	3	2	theme	lower	874:878	arg1	symptoms					880:887	lower symptoms	874:887	lower symptoms of colitis	874:898	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	2	3	from	assessment	529:538	arg1	tissue					578:583	the gut tissue	570:583	the gut tissue	570:583	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	2	4	theme	index	516:520	arg1	basis					475:479	the basis	471:479	the basis of the calculated disease activity index	471:520	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	3	5	theme	MPO	1012:1014	arg1	IL-6					986:989	IL-6	986:989	IL-6	986:989	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	3	5	theme	MPO	1012:1014	arg1	cytokines					962:970	pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO	945:1014	pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO	945:1014	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	3	5	theme	MPO	1012:1014	arg1	IL-1β					979:983	IL-1β	979:983	IL-1β	979:983	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	3	5	theme	MPO	1012:1014	arg1	TNF-α					972:976	TNF-α	972:976	TNF-α	972:976	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	3	5	theme	MPO	1012:1014	arg1	activity					1000:1007	the activity	996:1007	the activity of MPO	996:1014	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	0	6	theme	sodium-induced	89:102	arg1	inflammation					116:127	dextran sulfate sodium-induced acute bowel inflammation	73:127	dextran sulfate sodium-induced acute bowel inflammation	73:127	Composites of yeast glucan particles and curcumin lead to improvement of dextran sulfate sodium-induced acute bowel inflammation in rats.
33183601	1	7	theme	curcumin	315:322	arg1	curcumin					315:322	curcumin	315:322	curcumin	315:322	The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats.
33183601	1	7	theme	curcumin	315:322	arg1	composite					302:310	a pharmacological composite	284:310	a pharmacological composite of curcumin in GPs	284:329	The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats.
33183601	2	8	theme	enzymes	557:563	arg1	assessment					529:538	assessment	529:538	assessment of cytokines and enzymes from the gut tissue	529:583	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	3	9	theme	colitis	892:898	arg1	symptoms					880:887	lower symptoms	874:887	lower symptoms of colitis	874:898	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	0	10	theme	sulfate	81:87	arg1	inflammation					116:127	dextran sulfate sodium-induced acute bowel inflammation	73:127	dextran sulfate sodium-induced acute bowel inflammation	73:127	Composites of yeast glucan particles and curcumin lead to improvement of dextran sulfate sodium-induced acute bowel inflammation in rats.
33183601	1	11	from	effect	274:279	arg1	GPs					327:329	GPs	327:329	GPs	327:329	The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats.
33183601	1	11	from	effect	274:279	arg1	sodium					350:355	dextran sulfate sodium	334:355	dextran sulfate sodium induced colitis in rats	334:379	The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats.
33183601	2	12	theme	factor	602:607	arg1	α					609:609	tumor necrosis factor α	587:609	tumor necrosis factor α (TNF-α)	587:617	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	2	12	theme	factor	602:607	arg1	TNF-α					612:616	TNF-α	612:616	TNF-α	612:616	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	3	13	theme	GPs	795:797	arg1	GPs					795:797	GPs	795:797	GPs	795:797	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	3	13	theme	GPs	795:797	arg1	Composites					781:790	Composites	781:790	Composites of GPs with incorporated curcumin	781:824	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	2	14	theme	matrix	753:758	arg1	metalloproteinase					760:776	matrix metalloproteinase 9	753:778	matrix metalloproteinase 9	753:778	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	0	15	theme	bowel	110:114	arg1	inflammation					116:127	dextran sulfate sodium-induced acute bowel inflammation	73:127	dextran sulfate sodium-induced acute bowel inflammation	73:127	Composites of yeast glucan particles and curcumin lead to improvement of dextran sulfate sodium-induced acute bowel inflammation in rats.
33183601	2	16	theme	tumor	587:591	arg1	α					609:609	tumor necrosis factor α	587:609	tumor necrosis factor α (TNF-α)	587:617	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	2	16	theme	tumor	587:591	arg1	TNF-α					612:616	TNF-α	612:616	TNF-α	612:616	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	2	17	theme	necrosis	593:600	arg1	α					609:609	tumor necrosis factor α	587:609	tumor necrosis factor α (TNF-α)	587:617	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	2	17	theme	necrosis	593:600	arg1	TNF-α					612:616	TNF-α	612:616	TNF-α	612:616	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	1	18	theme	work	155:158	arg1	goal					142:145	The goal	138:145	The goal of this work	138:158	The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats.
33183601	0	19	theme	acute	104:108	arg1	inflammation					116:127	dextran sulfate sodium-induced acute bowel inflammation	73:127	dextran sulfate sodium-induced acute bowel inflammation	73:127	Composites of yeast glucan particles and curcumin lead to improvement of dextran sulfate sodium-induced acute bowel inflammation in rats.
33183601	2	20	theme	anti-inflammatory	404:420	arg1	effect					422:427	the anti-inflammatory effect	400:427	the anti-inflammatory effect of particular substances	400:452	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	1	21	theme	dextran	334:340	arg1	sodium					350:355	dextran sulfate sodium	334:355	dextran sulfate sodium induced colitis in rats	334:379	The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats.
33183601	3	22	theme	pro-inflammatory	945:960	arg1	IL-6					986:989	IL-6	986:989	IL-6	986:989	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	3	22	theme	pro-inflammatory	945:960	arg1	cytokines					962:970	pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO	945:1014	pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO	945:1014	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	3	22	theme	pro-inflammatory	945:960	arg1	IL-1β					979:983	IL-1β	979:983	IL-1β	979:983	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	3	22	theme	pro-inflammatory	945:960	arg1	activity					1000:1007	the activity	996:1007	the activity of MPO	996:1014	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	3	22	theme	pro-inflammatory	945:960	arg1	TNF-α					972:976	TNF-α	972:976	TNF-α	972:976	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	2	23	theme	gut	574:576	arg1	tissue					578:583	the gut tissue	570:583	the gut tissue	570:583	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	4	24	theme	pure	1099:1102	arg1	GPs					1104:1106	GPs	1104:1106	GPs	1104:1106	The anti-inflammatory effect of the composites was greater than those of pure GPs or curcumin.
33183601	3	25	with	Composites	781:790	arg1	curcumin					817:824	incorporated curcumin	804:824	incorporated curcumin	804:824	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	1	26	from	composite	302:310	arg1	GPs					327:329	GPs	327:329	GPs	327:329	The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats.
33183601	0	27	theme	glucan	20:25	arg1	particles					27:35	yeast glucan particles	14:35	yeast glucan particles	14:35	Composites of yeast glucan particles and curcumin lead to improvement of dextran sulfate sodium-induced acute bowel inflammation in rats.
33183601	2	28	dep	-1β	667:669	arg1	interleukin					651:661	growth factor β1, interleukin (IL)-1β	633:669	interleukin	651:661	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	3	29	theme	promising	833:841	arg1	results					843:849	promising results	833:849	promising results	833:849	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	1	30	from	GPs	327:329	arg1	effect					274:279	the beneficial effect	259:279	the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats	259:379	The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats.
33183601	0	31	theme	inflammation	116:127	arg1	improvement					58:68	improvement	58:68	improvement of dextran sulfate sodium-induced acute bowel inflammation in rats	58:135	Composites of yeast glucan particles and curcumin lead to improvement of dextran sulfate sodium-induced acute bowel inflammation in rats.
33183601	1	32	theme	sulfate	342:348	arg1	sodium					350:355	dextran sulfate sodium	334:355	dextran sulfate sodium induced colitis in rats	334:379	The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats.
33183601	2	33	theme	particular	432:441	arg1	substances					443:452	particular substances	432:452	particular substances	432:452	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	3	34	theme	incorporated	804:815	arg1	curcumin					817:824	incorporated curcumin	804:824	incorporated curcumin	804:824	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	2	35	theme	substances	443:452	arg1	effect					422:427	the anti-inflammatory effect	400:427	the anti-inflammatory effect of particular substances	400:452	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	2	36	theme	activity	507:514	arg1	index					516:520	the calculated disease activity index	484:520	the calculated disease activity index	484:520	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	0	37	theme	particles	27:35	arg1	Composites					0:9	Composites	0:9	Composites of yeast glucan particles and curcumin	0:48	Composites of yeast glucan particles and curcumin lead to improvement of dextran sulfate sodium-induced acute bowel inflammation in rats.
33183601	0	37	theme	particles	27:35	arg1	curcumin					41:48	curcumin	41:48	curcumin	41:48	Composites of yeast glucan particles and curcumin lead to improvement of dextran sulfate sodium-induced acute bowel inflammation in rats.
33183601	0	37	theme	particles	27:35	arg1	particles					27:35	yeast glucan particles	14:35	yeast glucan particles	14:35	Composites of yeast glucan particles and curcumin lead to improvement of dextran sulfate sodium-induced acute bowel inflammation in rats.
33183601	1	38	theme	beneficial	263:272	arg1	effect					274:279	the beneficial effect	259:279	the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats	259:379	The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats.
33183601	2	39	theme	disease	499:505	arg1	index					516:520	the calculated disease activity index	484:520	the calculated disease activity index	484:520	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	0	40	from	improvement	58:68	arg1	rats					132:135	rats	132:135	rats	132:135	Composites of yeast glucan particles and curcumin lead to improvement of dextran sulfate sodium-induced acute bowel inflammation in rats.
33183601	4	41	theme	composites	1062:1071	arg1	effect					1048:1053	The anti-inflammatory effect	1026:1053	The anti-inflammatory effect of the composites	1026:1071	The anti-inflammatory effect of the composites was greater than those of pure GPs or curcumin.
33183601	4	41	theme	composites	1062:1071	arg1	greater					1077:1083	greater	1077:1083	greater	1077:1083	The anti-inflammatory effect of the composites was greater than those of pure GPs or curcumin.
33183601	1	42	theme	yeast	191:195	arg1	GPs					215:217	GPs	215:217	GPs	215:217	The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats.
33183601	1	42	theme	yeast	191:195	arg1	particles					204:212	yeast glucan particles	191:212	yeast glucan particles (GPs)	191:218	The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats.
33183601	2	43	theme	calculated	488:497	arg1	index					516:520	the calculated disease activity index	484:520	the calculated disease activity index	484:520	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	2	44	theme	cytokines	543:551	arg1	assessment					529:538	assessment	529:538	assessment of cytokines and enzymes from the gut tissue	529:583	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	2	45	theme	β1	647:648	arg1	-1β					667:669	growth factor β1, interleukin (IL)-1β	633:669	-1β	667:669	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	0	46	theme	curcumin	41:48	arg1	Composites					0:9	Composites	0:9	Composites of yeast glucan particles and curcumin	0:48	Composites of yeast glucan particles and curcumin lead to improvement of dextran sulfate sodium-induced acute bowel inflammation in rats.
33183601	0	46	theme	curcumin	41:48	arg1	curcumin					41:48	curcumin	41:48	curcumin	41:48	Composites of yeast glucan particles and curcumin lead to improvement of dextran sulfate sodium-induced acute bowel inflammation in rats.
33183601	0	46	theme	curcumin	41:48	arg1	particles					27:35	yeast glucan particles	14:35	yeast glucan particles	14:35	Composites of yeast glucan particles and curcumin lead to improvement of dextran sulfate sodium-induced acute bowel inflammation in rats.
33183601	2	47	theme	effect	422:427	arg1	assessment					386:395	The assessment	382:395	The assessment of the anti-inflammatory effect of particular substances	382:452	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	2	48	theme	factor	640:645	arg1	-1β					667:669	growth factor β1, interleukin (IL)-1β	633:669	-1β	667:669	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	3	49	theme	cytokines	962:970	arg1	production					931:940	the production	927:940	the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO	927:1014	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	2	50	theme	growth	633:638	arg1	-1β					667:669	growth factor β1, interleukin (IL)-1β	633:669	-1β	667:669	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	2	51	theme	superoxide	702:711	arg1	dismutase					713:721	superoxide dismutase 2	702:723	superoxide dismutase 2	702:723	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	1	52	theme	glucan	197:202	arg1	GPs					215:217	GPs	215:217	GPs	215:217	The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats.
33183601	1	52	theme	glucan	197:202	arg1	particles					204:212	yeast glucan particles	191:212	yeast glucan particles (GPs)	191:218	The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats.
33183601	4	53	theme	anti-inflammatory	1030:1046	arg1	effect					1048:1053	The anti-inflammatory effect	1026:1053	The anti-inflammatory effect of the composites	1026:1071	The anti-inflammatory effect of the composites was greater than those of pure GPs or curcumin.
33183601	4	53	theme	anti-inflammatory	1030:1046	arg1	greater					1077:1083	greater	1077:1083	greater	1077:1083	The anti-inflammatory effect of the composites was greater than those of pure GPs or curcumin.
33183601	1	54	theme	particles	204:212	arg1	usability					178:186	the usability	174:186	the usability of yeast glucan particles (GPs)	174:218	The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats.
33183601	1	54	theme	particles	204:212	arg1	carriers					223:230	carriers	223:230	carriers for curcumin	223:243	The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats.
33183601	2	55	theme	IL	664:665	arg1	-1β					667:669	growth factor β1, interleukin (IL)-1β	633:669	-1β	667:669	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	2	56	from	tissue	578:583	arg1	assessment					529:538	assessment	529:538	assessment of cytokines and enzymes from the gut tissue	529:583	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	2	56	from	tissue	578:583	arg1	enzymes					557:563	enzymes	557:563	enzymes	557:563	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	2	56	from	tissue	578:583	arg1	cytokines					543:551	cytokines	543:551	cytokines	543:551	The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9.
33183601	1	57	theme	pharmacological	286:300	arg1	curcumin					315:322	curcumin	315:322	curcumin	315:322	The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats.
33183601	1	57	theme	pharmacological	286:300	arg1	composite					302:310	a pharmacological composite	284:310	a pharmacological composite of curcumin in GPs	284:329	The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats.
33183601	0	58	theme	yeast	14:18	arg1	particles					27:35	yeast glucan particles	14:35	yeast glucan particles	14:35	Composites of yeast glucan particles and curcumin lead to improvement of dextran sulfate sodium-induced acute bowel inflammation in rats.
33183601	3	59	dep	cytokines	962:970	arg1	IL-6					986:989	IL-6	986:989	IL-6	986:989	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	3	59	dep	cytokines	962:970	arg1	cytokines					962:970	pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO	945:1014	pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO	945:1014	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	3	59	dep	cytokines	962:970	arg1	IL-1β					979:983	IL-1β	979:983	IL-1β	979:983	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	3	59	dep	cytokines	962:970	arg1	activity					1000:1007	the activity	996:1007	the activity of MPO	996:1014	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	3	59	dep	cytokines	962:970	arg1	TNF-α					972:976	TNF-α	972:976	TNF-α	972:976	Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well.
33183601	1	60	from	colitis	365:371	arg1	rats					376:379	rats	376:379	rats	376:379	The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats.
34774866	5	0	theme	stable	658:663	arg1	hydrogel					672:679	stable CS/Pec hydrogel	658:679	stable CS/Pec hydrogel	658:679	The used strategy created stable CS/Pec hydrogel against disintegration and water dissolution.
34774866	1	1	theme	natural	169:175	arg1	carbohydrates					177:189	natural carbohydrates	169:189	natural carbohydrates	169:189	Biodegradable and eco-friendly adsorbents composed of natural carbohydrates have been used to replace carbon-based materials.
34774866	10	2	theme	0.83 g/L	1229:1236	arg1	pH 4.0					1256:1261	pH 4.0	1256:1261	pH 4.0	1256:1261	The maximum removal capacities (qm) predicted by the Langmuir and Sips isotherms achieved 108.2 and 97.55 mg/g at 0.83 g/L adsorbent dosage (pH 4.0).
34774866	10	2	theme	0.83 g/L	1229:1236	arg1	dosage					1248:1253	0.83 g/L adsorbent dosage	1229:1253	0.83 g/L adsorbent dosage (pH 4.0)	1229:1262	The maximum removal capacities (qm) predicted by the Langmuir and Sips isotherms achieved 108.2 and 97.55 mg/g at 0.83 g/L adsorbent dosage (pH 4.0).
34774866	5	3	theme	CS/Pec	665:670	arg1	hydrogel					672:679	stable CS/Pec hydrogel	658:679	stable CS/Pec hydrogel	658:679	The used strategy created stable CS/Pec hydrogel against disintegration and water dissolution.
34774866	3	4	theme	crosslinking	509:520	arg1	chemistries					496:506	toxic chemistries	490:506	toxic chemistries (crosslinking agents)	490:528	The physical CS/Pec hydrogel was prepared by blending aqueous CS and Pec solutions at 65 °C, preventing the use of toxic chemistries (crosslinking agents).
34774866	3	4	theme	crosslinking	509:520	arg1	agents					522:527	crosslinking agents	509:527	crosslinking agents	509:527	The physical CS/Pec hydrogel was prepared by blending aqueous CS and Pec solutions at 65 °C, preventing the use of toxic chemistries (crosslinking agents).
34774866	0	5	theme	aqueous	96:102	arg1	solutions					104:112	aqueous solutions	96:112	aqueous solutions	96:112	Natural carbohydrate-based thermosensitive chitosan/pectin adsorbent for removal of Pb(II) from aqueous solutions.
34774866	2	6	theme	chitosan/pectin	290:304	arg1	hydrogel					315:322	a natural carbohydrate-based chitosan/pectin (CS/Pec) hydrogel	261:322	a natural carbohydrate-based chitosan/pectin (CS/Pec) hydrogel adsorbent to remove Pb(II) from aqueous solutions	261:372	This study presents a natural carbohydrate-based chitosan/pectin (CS/Pec) hydrogel adsorbent to remove Pb(II) from aqueous solutions.
34774866	11	7	theme	differential	1382:1393	arg1	DSC					1417:1419	DSC	1417:1419	DSC	1417:1419	The hydrogel/Pb(II) pair was characterized by scanning electron microscopy (SEM), X-ray dispersive energy (EDS), and differential scanning calorimetry (DSC).
34774866	11	7	theme	differential	1382:1393	arg1	calorimetry					1404:1414	differential scanning calorimetry	1382:1414	differential scanning calorimetry (DSC)	1382:1420	The hydrogel/Pb(II) pair was characterized by scanning electron microscopy (SEM), X-ray dispersive energy (EDS), and differential scanning calorimetry (DSC).
34774866	2	8	theme	adsorbent	324:332	arg1	hydrogel					315:322	a natural carbohydrate-based chitosan/pectin (CS/Pec) hydrogel	261:322	a natural carbohydrate-based chitosan/pectin (CS/Pec) hydrogel adsorbent to remove Pb(II) from aqueous solutions	261:372	This study presents a natural carbohydrate-based chitosan/pectin (CS/Pec) hydrogel adsorbent to remove Pb(II) from aqueous solutions.
34774866	9	9	theme	experimental	1082:1093	arg1	data					1095:1098	the experimental data	1078:1098	the experimental data	1078:1098	The adsorption kinetic and equilibrium studies indicated that the Elovich and pseudo-second-order models adjusted well to the experimental data, respectively.
34774866	3	10	theme	physical	379:386	arg1	hydrogel					395:402	The physical CS/Pec hydrogel	375:402	The physical CS/Pec hydrogel	375:402	The physical CS/Pec hydrogel was prepared by blending aqueous CS and Pec solutions at 65 °C, preventing the use of toxic chemistries (crosslinking agents).
34774866	11	11	theme	scanning	1311:1318	arg1	SEM					1341:1343	SEM	1341:1343	SEM	1341:1343	The hydrogel/Pb(II) pair was characterized by scanning electron microscopy (SEM), X-ray dispersive energy (EDS), and differential scanning calorimetry (DSC).
34774866	11	11	theme	scanning	1311:1318	arg1	microscopy					1329:1338	scanning electron microscopy	1311:1338	scanning electron microscopy (SEM)	1311:1344	The hydrogel/Pb(II) pair was characterized by scanning electron microscopy (SEM), X-ray dispersive energy (EDS), and differential scanning calorimetry (DSC).
34774866	8	12	theme	ionized	896:902	arg1	sites					904:908	These ionized sites	890:908	These ionized sites	890:908	These ionized sites bind Pb(II) ions, promoting their adsorption.
34774866	4	13	theme	CS/Pec	598:603	arg1	blend					605:609	CS/Pec blend	598:609	CS/Pec blend	598:609	The thermosensitive CS/Pec hydrogel was quickly created by cooling CS/Pec blend at room temperature.
34774866	5	14	dep	disintegration	689:702	arg1	dissolution					714:724	dissolution	714:724	dissolution	714:724	The used strategy created stable CS/Pec hydrogel against disintegration and water dissolution.
34774866	2	15	theme	aqueous	356:362	arg1	solutions					364:372	aqueous solutions	356:372	aqueous solutions	356:372	This study presents a natural carbohydrate-based chitosan/pectin (CS/Pec) hydrogel adsorbent to remove Pb(II) from aqueous solutions.
34774866	11	16	theme	electron	1320:1327	arg1	SEM					1341:1343	SEM	1341:1343	SEM	1341:1343	The hydrogel/Pb(II) pair was characterized by scanning electron microscopy (SEM), X-ray dispersive energy (EDS), and differential scanning calorimetry (DSC).
34774866	11	16	theme	electron	1320:1327	arg1	microscopy					1329:1338	scanning electron microscopy	1311:1338	scanning electron microscopy (SEM)	1311:1344	The hydrogel/Pb(II) pair was characterized by scanning electron microscopy (SEM), X-ray dispersive energy (EDS), and differential scanning calorimetry (DSC).
34774866	13	17	theme	low	1551:1553	arg1	potential					1555:1563	low potential	1551:1563	low potential for reusability	1551:1579	Therefore, the adsorbent was not recovered, showing low potential for reusability.
34774866	6	18	theme	as-prepared	731:741	arg1	hydrogel					743:750	The as-prepared hydrogel	727:750	The as-prepared hydrogel	727:750	The as-prepared hydrogel was characterized by infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
34774866	10	19	theme	maximum	1119:1125	arg1	qm					1147:1148	qm	1147:1148	qm	1147:1148	The maximum removal capacities (qm) predicted by the Langmuir and Sips isotherms achieved 108.2 and 97.55 mg/g at 0.83 g/L adsorbent dosage (pH 4.0).
34774866	10	19	theme	maximum	1119:1125	arg1	capacities					1135:1144	The maximum removal capacities	1115:1144	The maximum removal capacities (qm) predicted by the Langmuir and Sips isotherms	1115:1194	The maximum removal capacities (qm) predicted by the Langmuir and Sips isotherms achieved 108.2 and 97.55 mg/g at 0.83 g/L adsorbent dosage (pH 4.0).
34774866	5	20	theme	used	636:639	arg1	strategy					641:648	The used strategy	632:648	The used strategy	632:648	The used strategy created stable CS/Pec hydrogel against disintegration and water dissolution.
34774866	2	21	theme	CS/Pec	307:312	arg1	hydrogel					315:322	a natural carbohydrate-based chitosan/pectin (CS/Pec) hydrogel	261:322	a natural carbohydrate-based chitosan/pectin (CS/Pec) hydrogel adsorbent to remove Pb(II) from aqueous solutions	261:372	This study presents a natural carbohydrate-based chitosan/pectin (CS/Pec) hydrogel adsorbent to remove Pb(II) from aqueous solutions.
34774866	3	22	theme	toxic	490:494	arg1	chemistries					496:506	toxic chemistries	490:506	toxic chemistries (crosslinking agents)	490:528	The physical CS/Pec hydrogel was prepared by blending aqueous CS and Pec solutions at 65 °C, preventing the use of toxic chemistries (crosslinking agents).
34774866	3	22	theme	toxic	490:494	arg1	agents					522:527	crosslinking agents	509:527	crosslinking agents	509:527	The physical CS/Pec hydrogel was prepared by blending aqueous CS and Pec solutions at 65 °C, preventing the use of toxic chemistries (crosslinking agents).
34774866	0	23	theme	carbohydrate-based	8:25	arg1	chitosan/pectin					43:57	Natural carbohydrate-based thermosensitive chitosan/pectin	0:57	Natural carbohydrate-based thermosensitive chitosan/pectin	0:57	Natural carbohydrate-based thermosensitive chitosan/pectin adsorbent for removal of Pb(II) from aqueous solutions.
34774866	9	24	dep	indicated	1003:1011	arg1	models					1054:1059	the Elovich and pseudo-second-order models	1018:1059	the Elovich and pseudo-second-order models adjusted well to the experimental data, respectively	1018:1112	The adsorption kinetic and equilibrium studies indicated that the Elovich and pseudo-second-order models adjusted well to the experimental data, respectively.
34774866	10	25	theme	removal	1127:1133	arg1	qm					1147:1148	qm	1147:1148	qm	1147:1148	The maximum removal capacities (qm) predicted by the Langmuir and Sips isotherms achieved 108.2 and 97.55 mg/g at 0.83 g/L adsorbent dosage (pH 4.0).
34774866	10	25	theme	removal	1127:1133	arg1	capacities					1135:1144	The maximum removal capacities	1115:1144	The maximum removal capacities (qm) predicted by the Langmuir and Sips isotherms	1115:1194	The maximum removal capacities (qm) predicted by the Langmuir and Sips isotherms achieved 108.2 and 97.55 mg/g at 0.83 g/L adsorbent dosage (pH 4.0).
34774866	0	26	theme	Natural	0:6	arg1	chitosan/pectin					43:57	Natural carbohydrate-based thermosensitive chitosan/pectin	0:57	Natural carbohydrate-based thermosensitive chitosan/pectin	0:57	Natural carbohydrate-based thermosensitive chitosan/pectin adsorbent for removal of Pb(II) from aqueous solutions.
34774866	6	27	theme	thermogravimetric	806:822	arg1	analysis					824:831	thermogravimetric analysis	806:831	thermogravimetric analysis (TGA)	806:837	The as-prepared hydrogel was characterized by infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
34774866	6	27	theme	thermogravimetric	806:822	arg1	TGA					834:836	TGA	834:836	TGA	834:836	The as-prepared hydrogel was characterized by infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
34774866	7	28	theme	1.688 mmol	858:867	arg1	-COO-					869:873	1.688 mmol -COO-	858:873	1.688 mmol -COO-	858:873	The adsorbent had 1.688 mmol -COO- for each gram.
34774866	3	29	theme	Pec	444:446	arg1	solutions					448:456	aqueous CS and Pec solutions	429:456	aqueous CS and Pec solutions	429:456	The physical CS/Pec hydrogel was prepared by blending aqueous CS and Pec solutions at 65 °C, preventing the use of toxic chemistries (crosslinking agents).
34774866	3	30	theme	CS/Pec	388:393	arg1	hydrogel					395:402	The physical CS/Pec hydrogel	375:402	The physical CS/Pec hydrogel	375:402	The physical CS/Pec hydrogel was prepared by blending aqueous CS and Pec solutions at 65 °C, preventing the use of toxic chemistries (crosslinking agents).
34774866	7	31	contain	had	854:856	arg1	adsorbent					844:852	adsorbent	844:852	adsorbent	844:852	The adsorbent had 1.688 mmol -COO- for each gram.
34774866	7	31	contain	had	854:856	arg2	-COO-					869:873	1.688 mmol -COO-	858:873	1.688 mmol -COO-	858:873	The adsorbent had 1.688 mmol -COO- for each gram.
34774866	8	32	theme	Pb	915:916	arg1	ions					922:925	Pb(II) ions	915:925	Pb(II) ions	915:925	These ionized sites bind Pb(II) ions, promoting their adsorption.
34774866	0	33	theme	thermosensitive	27:41	arg1	chitosan/pectin					43:57	Natural carbohydrate-based thermosensitive chitosan/pectin	0:57	Natural carbohydrate-based thermosensitive chitosan/pectin	0:57	Natural carbohydrate-based thermosensitive chitosan/pectin adsorbent for removal of Pb(II) from aqueous solutions.
34774866	11	34	theme	scanning	1395:1402	arg1	DSC					1417:1419	DSC	1417:1419	DSC	1417:1419	The hydrogel/Pb(II) pair was characterized by scanning electron microscopy (SEM), X-ray dispersive energy (EDS), and differential scanning calorimetry (DSC).
34774866	11	34	theme	scanning	1395:1402	arg1	calorimetry					1404:1414	differential scanning calorimetry	1382:1414	differential scanning calorimetry (DSC)	1382:1420	The hydrogel/Pb(II) pair was characterized by scanning electron microscopy (SEM), X-ray dispersive energy (EDS), and differential scanning calorimetry (DSC).
34774866	1	35	theme	carbon-based	217:228	arg1	materials					230:238	carbon-based materials	217:238	carbon-based materials	217:238	Biodegradable and eco-friendly adsorbents composed of natural carbohydrates have been used to replace carbon-based materials.
34774866	3	36	theme	aqueous	429:435	arg1	solutions					448:456	aqueous CS and Pec solutions	429:456	aqueous CS and Pec solutions	429:456	The physical CS/Pec hydrogel was prepared by blending aqueous CS and Pec solutions at 65 °C, preventing the use of toxic chemistries (crosslinking agents).
34774866	3	37	theme	CS	437:438	arg1	solutions					448:456	aqueous CS and Pec solutions	429:456	aqueous CS and Pec solutions	429:456	The physical CS/Pec hydrogel was prepared by blending aqueous CS and Pec solutions at 65 °C, preventing the use of toxic chemistries (crosslinking agents).
34774866	11	38	theme	dispersive	1353:1362	arg1	EDS					1372:1374	EDS	1372:1374	EDS	1372:1374	The hydrogel/Pb(II) pair was characterized by scanning electron microscopy (SEM), X-ray dispersive energy (EDS), and differential scanning calorimetry (DSC).
34774866	11	38	theme	dispersive	1353:1362	arg1	energy					1364:1369	X-ray dispersive energy	1347:1369	X-ray dispersive energy (EDS)	1347:1375	The hydrogel/Pb(II) pair was characterized by scanning electron microscopy (SEM), X-ray dispersive energy (EDS), and differential scanning calorimetry (DSC).
34774866	12	39	theme	Pb	1480:1481	arg1	adsorption					1487:1496	the Pb(II) adsorption	1476:1496	the Pb(II) adsorption	1476:1496	The chemisorption seems to play an essential role in the Pb(II) adsorption.
34774866	9	40	theme	equilibrium	983:993	arg1	studies					995:1001	The adsorption kinetic and equilibrium studies	956:1001	studies	995:1001	The adsorption kinetic and equilibrium studies indicated that the Elovich and pseudo-second-order models adjusted well to the experimental data, respectively.
34774866	11	41	theme	X-ray	1347:1351	arg1	EDS					1372:1374	EDS	1372:1374	EDS	1372:1374	The hydrogel/Pb(II) pair was characterized by scanning electron microscopy (SEM), X-ray dispersive energy (EDS), and differential scanning calorimetry (DSC).
34774866	11	41	theme	X-ray	1347:1351	arg1	energy					1364:1369	X-ray dispersive energy	1347:1369	X-ray dispersive energy (EDS)	1347:1375	The hydrogel/Pb(II) pair was characterized by scanning electron microscopy (SEM), X-ray dispersive energy (EDS), and differential scanning calorimetry (DSC).
34774866	10	42	theme	Langmuir	1168:1175	arg1	isotherms					1186:1194	the Langmuir and Sips isotherms	1164:1194	isotherms	1186:1194	The maximum removal capacities (qm) predicted by the Langmuir and Sips isotherms achieved 108.2 and 97.55 mg/g at 0.83 g/L adsorbent dosage (pH 4.0).
34774866	1	43	theme	Biodegradable	115:127	arg1	adsorbents					146:155	Biodegradable and eco-friendly adsorbents	115:155	Biodegradable and eco-friendly adsorbents composed of natural carbohydrates	115:189	Biodegradable and eco-friendly adsorbents composed of natural carbohydrates have been used to replace carbon-based materials.
34774866	9	44	theme	Elovich	1022:1028	arg1	models					1054:1059	the Elovich and pseudo-second-order models	1018:1059	the Elovich and pseudo-second-order models adjusted well to the experimental data, respectively	1018:1112	The adsorption kinetic and equilibrium studies indicated that the Elovich and pseudo-second-order models adjusted well to the experimental data, respectively.
34774866	4	45	theme	CS/Pec	551:556	arg1	hydrogel					558:565	The thermosensitive CS/Pec hydrogel	531:565	The thermosensitive CS/Pec hydrogel	531:565	The thermosensitive CS/Pec hydrogel was quickly created by cooling CS/Pec blend at room temperature.
34774866	11	46	theme	hydrogel/Pb	1269:1279	arg1	pair					1285:1288	The hydrogel/Pb(II) pair	1265:1288	The hydrogel/Pb(II) pair	1265:1288	The hydrogel/Pb(II) pair was characterized by scanning electron microscopy (SEM), X-ray dispersive energy (EDS), and differential scanning calorimetry (DSC).
34774866	0	47	dep	adsorbent	59:67	arg1	chitosan/pectin					43:57	Natural carbohydrate-based thermosensitive chitosan/pectin	0:57	Natural carbohydrate-based thermosensitive chitosan/pectin	0:57	Natural carbohydrate-based thermosensitive chitosan/pectin adsorbent for removal of Pb(II) from aqueous solutions.
34774866	3	48	theme	chemistries	496:506	arg1	use					483:485	the use	479:485	the use of toxic chemistries (crosslinking agents)	479:528	The physical CS/Pec hydrogel was prepared by blending aqueous CS and Pec solutions at 65 °C, preventing the use of toxic chemistries (crosslinking agents).
34774866	4	49	theme	thermosensitive	535:549	arg1	hydrogel					558:565	The thermosensitive CS/Pec hydrogel	531:565	The thermosensitive CS/Pec hydrogel	531:565	The thermosensitive CS/Pec hydrogel was quickly created by cooling CS/Pec blend at room temperature.
34774866	0	50	from	solutions	104:112	arg1	removal					73:79	removal	73:79	removal of Pb(II) from aqueous solutions	73:112	Natural carbohydrate-based thermosensitive chitosan/pectin adsorbent for removal of Pb(II) from aqueous solutions.
34774866	1	51	theme	eco-friendly	133:144	arg1	adsorbents					146:155	Biodegradable and eco-friendly adsorbents	115:155	Biodegradable and eco-friendly adsorbents composed of natural carbohydrates	115:189	Biodegradable and eco-friendly adsorbents composed of natural carbohydrates have been used to replace carbon-based materials.
34774866	9	52	theme	pseudo-second-order	1034:1052	arg1	models					1054:1059	the Elovich and pseudo-second-order models	1018:1059	the Elovich and pseudo-second-order models adjusted well to the experimental data, respectively	1018:1112	The adsorption kinetic and equilibrium studies indicated that the Elovich and pseudo-second-order models adjusted well to the experimental data, respectively.
34774866	4	53	theme	room	614:617	arg1	temperature					619:629	room temperature	614:629	room temperature	614:629	The thermosensitive CS/Pec hydrogel was quickly created by cooling CS/Pec blend at room temperature.
34774866	12	54	theme	essential	1458:1466	arg1	role					1468:1471	an essential role	1455:1471	an essential role	1455:1471	The chemisorption seems to play an essential role in the Pb(II) adsorption.
34774866	1	55	used	used	201:204	arg2	adsorbents					146:155	Biodegradable and eco-friendly adsorbents	115:155	Biodegradable and eco-friendly adsorbents composed of natural carbohydrates	115:189	Biodegradable and eco-friendly adsorbents composed of natural carbohydrates have been used to replace carbon-based materials.
34774866	6	56	theme	infrared	773:780	arg1	FTIR					796:799	FTIR	796:799	FTIR	796:799	The as-prepared hydrogel was characterized by infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
34774866	6	56	theme	infrared	773:780	arg1	spectroscopy					782:793	infrared spectroscopy	773:793	infrared spectroscopy (FTIR)	773:800	The as-prepared hydrogel was characterized by infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
34774866	2	57	theme	carbohydrate-based	271:288	arg1	hydrogel					315:322	a natural carbohydrate-based chitosan/pectin (CS/Pec) hydrogel	261:322	a natural carbohydrate-based chitosan/pectin (CS/Pec) hydrogel adsorbent to remove Pb(II) from aqueous solutions	261:372	This study presents a natural carbohydrate-based chitosan/pectin (CS/Pec) hydrogel adsorbent to remove Pb(II) from aqueous solutions.
34774866	10	58	theme	adsorbent	1238:1246	arg1	pH 4.0					1256:1261	pH 4.0	1256:1261	pH 4.0	1256:1261	The maximum removal capacities (qm) predicted by the Langmuir and Sips isotherms achieved 108.2 and 97.55 mg/g at 0.83 g/L adsorbent dosage (pH 4.0).
34774866	10	58	theme	adsorbent	1238:1246	arg1	dosage					1248:1253	0.83 g/L adsorbent dosage	1229:1253	0.83 g/L adsorbent dosage (pH 4.0)	1229:1262	The maximum removal capacities (qm) predicted by the Langmuir and Sips isotherms achieved 108.2 and 97.55 mg/g at 0.83 g/L adsorbent dosage (pH 4.0).
34774866	0	59	theme	Pb	84:85	arg1	removal					73:79	removal	73:79	removal of Pb(II) from aqueous solutions	73:112	Natural carbohydrate-based thermosensitive chitosan/pectin adsorbent for removal of Pb(II) from aqueous solutions.
34774866	2	60	theme	natural	263:269	arg1	hydrogel					315:322	a natural carbohydrate-based chitosan/pectin (CS/Pec) hydrogel	261:322	a natural carbohydrate-based chitosan/pectin (CS/Pec) hydrogel adsorbent to remove Pb(II) from aqueous solutions	261:372	This study presents a natural carbohydrate-based chitosan/pectin (CS/Pec) hydrogel adsorbent to remove Pb(II) from aqueous solutions.
34774866	10	61	theme	Sips	1181:1184	arg1	isotherms					1186:1194	the Langmuir and Sips isotherms	1164:1194	isotherms	1186:1194	The maximum removal capacities (qm) predicted by the Langmuir and Sips isotherms achieved 108.2 and 97.55 mg/g at 0.83 g/L adsorbent dosage (pH 4.0).
32460846	3	0	theme	embedded	516:523	arg1	NPs					511:513	NPs	511:513	NPs	511:513	METHODS We developed a nanocomposite-hydrogel (NP-H) comprised of poly-L-lactide nanoparticles (NPs) embedded in gelatin/hyaluronic acid (Gel/HA) hydrogel as a delivery vehicle for MSC-CM.
32460846	3	0	theme	embedded	516:523	arg1	nanoparticles					496:508	poly-L-lactide nanoparticles	481:508	poly-L-lactide nanoparticles (NPs) embedded in gelatin/hyaluronic acid (Gel/HA) hydrogel as a delivery vehicle for MSC-CM	481:601	METHODS We developed a nanocomposite-hydrogel (NP-H) comprised of poly-L-lactide nanoparticles (NPs) embedded in gelatin/hyaluronic acid (Gel/HA) hydrogel as a delivery vehicle for MSC-CM.
32460846	5	1	theme	composite	932:940	arg1	matrix					942:947	a composite matrix	930:947	a composite matrix followed by physiochemical characterization	930:991	Then we prepared a composite matrix followed by physiochemical characterization and functional assays were performed.
32460846	11	2	dep	CONCLUSION	1754:1763	arg1	observed					1768:1775	observed	1768:1775	observed that sustained release of MSC-CM is required to prevent dose-dependent cytotoxicity	1768:1859	CONCLUSION We observed that sustained release of MSC-CM is required to prevent dose-dependent cytotoxicity.
32460846	3	3	from	embedded	516:523	arg1	hydrogel					561:568	gelatin/hyaluronic acid (Gel/HA) hydrogel	528:568	gelatin/hyaluronic acid (Gel/HA) hydrogel as a delivery vehicle for MSC-CM	528:601	METHODS We developed a nanocomposite-hydrogel (NP-H) comprised of poly-L-lactide nanoparticles (NPs) embedded in gelatin/hyaluronic acid (Gel/HA) hydrogel as a delivery vehicle for MSC-CM.
32460846	2	4	theme	systems	357:363	arg1	establishment					320:332	the establishment	316:332	the establishment of controlled delivery systems	316:363	This has been clearly demonstrated by in vivo applications of MSC-CM, but the establishment of controlled delivery systems is an unmet requirement for clinical translation.
32460846	2	4	theme	systems	357:363	arg1	requirement					377:387	an unmet requirement	368:387	an unmet requirement for clinical translation	368:412	This has been clearly demonstrated by in vivo applications of MSC-CM, but the establishment of controlled delivery systems is an unmet requirement for clinical translation.
32460846	5	5	theme	physiochemical	961:974	arg1	characterization					976:991	physiochemical characterization	961:991	physiochemical characterization	961:991	Then we prepared a composite matrix followed by physiochemical characterization and functional assays were performed.
32460846	7	6	theme	Functional	1257:1266	arg1	evaluation					1268:1277	Functional evaluation	1257:1277	Functional evaluation of the composites	1257:1295	Functional evaluation of the composites was determined using MSCs and human fibroblasts (HFFs).
32460846	3	7	theme	gelatin/hyaluronic	528:545	arg1	Gel/HA					553:558	Gel/HA	553:558	Gel/HA	553:558	METHODS We developed a nanocomposite-hydrogel (NP-H) comprised of poly-L-lactide nanoparticles (NPs) embedded in gelatin/hyaluronic acid (Gel/HA) hydrogel as a delivery vehicle for MSC-CM.
32460846	3	7	theme	gelatin/hyaluronic	528:545	arg1	acid					547:550	gelatin/hyaluronic acid	528:550	gelatin/hyaluronic acid (Gel/HA) hydrogel as a delivery vehicle for MSC-CM	528:601	METHODS We developed a nanocomposite-hydrogel (NP-H) comprised of poly-L-lactide nanoparticles (NPs) embedded in gelatin/hyaluronic acid (Gel/HA) hydrogel as a delivery vehicle for MSC-CM.
32460846	4	8	theme	corresponding	762:774	arg1	CM					776:777	the corresponding CM	758:777	the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers	758:910	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	2	9	theme	MSC-CM	304:309	arg1	applications					288:299	in vivo applications	280:299	in vivo applications of MSC-CM	280:309	This has been clearly demonstrated by in vivo applications of MSC-CM, but the establishment of controlled delivery systems is an unmet requirement for clinical translation.
32460846	10	10	theme	10	1689:1690	arg1	%					1691:1691	%	1691:1691	%	1691:1691	Cell activities reduced considerably for all groups, except 10% ScCM-NP-H, which displayed a significant increase over time.
32460846	3	11	theme	delivery	575:582	arg1	vehicle					584:590	a delivery vehicle	573:590	a delivery vehicle for MSC-CM	573:601	METHODS We developed a nanocomposite-hydrogel (NP-H) comprised of poly-L-lactide nanoparticles (NPs) embedded in gelatin/hyaluronic acid (Gel/HA) hydrogel as a delivery vehicle for MSC-CM.
32460846	9	12	theme	ScCM-NP-H	1554:1562	arg1	extracts					1542:1549	5% and 10% extracts	1531:1549	5% and 10% extracts of ScCM-NP-H and SfCM-NP-H	1531:1576	The cells treated in vitro with 5% and 10% extracts of ScCM-NP-H and SfCM-NP-H exhibited a dose- and duration-dependent response.
32460846	9	13	theme	%	1532:1532	arg1	extracts					1542:1549	5% and 10% extracts	1531:1549	5% and 10% extracts of ScCM-NP-H and SfCM-NP-H	1531:1576	The cells treated in vitro with 5% and 10% extracts of ScCM-NP-H and SfCM-NP-H exhibited a dose- and duration-dependent response.
32460846	12	14	theme	proposed	1866:1873	arg1	hydrogel					1889:1896	The proposed nanocomposite hydrogel	1862:1896	The proposed nanocomposite hydrogel for MSC-CM delivery	1862:1916	The proposed nanocomposite hydrogel for MSC-CM delivery can open up a new array for its clinical application.
32460846	6	15	dep	porosity	1116:1123	arg1	%					1159:1159	> 1500%	1153:1159	> 1500%	1153:1159	RESULTS Nanocomposite hydrogel displayed an even distribution of NPs along with high porosity (> 60%) and swelling ratios > 1500%, while its protein release pattern corresponded to a mix of degradation and diffusion kinetics.
32460846	4	16	theme	serum-free	716:725	arg1	SFM					735:737	SFM	735:737	SFM	735:737	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	4	16	theme	serum-free	716:725	arg1	medium					727:732	serum-free medium	716:732	serum-free medium (SFM)	716:738	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	6	17	theme	kinetics	1247:1254	arg1	mix					1214:1216	a mix	1212:1216	a mix of degradation and diffusion kinetics	1212:1254	RESULTS Nanocomposite hydrogel displayed an even distribution of NPs along with high porosity (> 60%) and swelling ratios > 1500%, while its protein release pattern corresponded to a mix of degradation and diffusion kinetics.
32460846	4	18	theme	culture	628:634	arg1	conditions					636:645	the culture conditions	624:645	the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM)	624:738	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	10	19	theme	significant	1722:1732	arg1	increase					1734:1741	a significant increase	1720:1741	a significant increase	1720:1741	Cell activities reduced considerably for all groups, except 10% ScCM-NP-H, which displayed a significant increase over time.
32460846	10	20	theme	Cell	1629:1632	arg1	activities					1634:1643	Cell activities	1629:1643	Cell activities	1629:1643	Cell activities reduced considerably for all groups, except 10% ScCM-NP-H, which displayed a significant increase over time.
32460846	1	21	theme	bioactive	168:176	arg1	factors					178:184	bioactive factors	168:184	bioactive factors that act synergistically to induce therapeutic effects	168:239	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM) contains a cocktail of bioactive factors that act synergistically to induce therapeutic effects.
32460846	6	22	dep	RESULTS	1031:1037	arg1	displayed					1062:1070	displayed	1062:1070	displayed	1062:1070	RESULTS Nanocomposite hydrogel displayed an even distribution of NPs along with high porosity (> 60%) and swelling ratios > 1500%, while its protein release pattern corresponded to a mix of degradation and diffusion kinetics.
32460846	3	23	dep	METHODS	415:421	arg1	We					423:424	We	423:424	We	423:424	METHODS We developed a nanocomposite-hydrogel (NP-H) comprised of poly-L-lactide nanoparticles (NPs) embedded in gelatin/hyaluronic acid (Gel/HA) hydrogel as a delivery vehicle for MSC-CM.
32460846	12	24	theme	clinical	1950:1957	arg1	application					1959:1969	its clinical application	1946:1969	its clinical application	1946:1969	The proposed nanocomposite hydrogel for MSC-CM delivery can open up a new array for its clinical application.
32460846	2	25	theme	in	280:281	arg1	applications					288:299	in vivo applications	280:299	in vivo applications of MSC-CM	280:309	This has been clearly demonstrated by in vivo applications of MSC-CM, but the establishment of controlled delivery systems is an unmet requirement for clinical translation.
32460846	8	26	theme	ScCM-NP-H	1457:1465	arg1	groups					1467:1472	ScCM-NP-H groups	1457:1472	ScCM-NP-H groups having maximum activity	1457:1496	The cells seeded directly onto the composites displayed increasing metabolic activities over time, with ScCM-NP-H groups having maximum activity.
32460846	1	27	theme	factors	178:184	arg1	cocktail					156:163	a cocktail	154:163	a cocktail of bioactive factors that act synergistically to induce therapeutic effects	154:239	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM) contains a cocktail of bioactive factors that act synergistically to induce therapeutic effects.
32460846	1	28	contain	contains	145:152	arg1	MSC-CM					137:142	MSC-CM	137:142	MSC-CM	137:142	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM) contains a cocktail of bioactive factors that act synergistically to induce therapeutic effects.
32460846	1	28	contain	contains	145:152	arg1	medium					129:134	BACKGROUND Mesenchymal stromal cell conditioned medium	81:134	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM)	81:143	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM) contains a cocktail of bioactive factors that act synergistically to induce therapeutic effects.
32460846	1	28	contain	contains	145:152	arg2	cocktail					156:163	a cocktail	154:163	a cocktail of bioactive factors that act synergistically to induce therapeutic effects	154:239	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM) contains a cocktail of bioactive factors that act synergistically to induce therapeutic effects.
32460846	6	29	theme	NPs	1096:1098	arg1	distribution					1080:1091	an even distribution	1072:1091	an even distribution of NPs	1072:1098	RESULTS Nanocomposite hydrogel displayed an even distribution of NPs along with high porosity (> 60%) and swelling ratios > 1500%, while its protein release pattern corresponded to a mix of degradation and diffusion kinetics.
32460846	4	30	theme	marrow-derived	656:669	arg1	MSCs					671:674	bone marrow-derived MSCs	651:674	bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM)	651:738	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	1	31	theme	Mesenchymal	92:102	arg1	MSC-CM					137:142	MSC-CM	137:142	MSC-CM	137:142	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM) contains a cocktail of bioactive factors that act synergistically to induce therapeutic effects.
32460846	1	31	theme	Mesenchymal	92:102	arg1	medium					129:134	BACKGROUND Mesenchymal stromal cell conditioned medium	81:134	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM)	81:143	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM) contains a cocktail of bioactive factors that act synergistically to induce therapeutic effects.
32460846	4	32	dep	CM	776:777	arg1	medium					809:814	serum-containing conditioned medium	780:814	serum-containing conditioned medium (ScCM)	780:821	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	4	32	dep	CM	776:777	arg1	SfCM					858:861	SfCM	858:861	SfCM	858:861	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	4	32	dep	CM	776:777	arg1	medium					850:855	serum-free conditioned medium	827:855	serum-free conditioned medium (SfCM)	827:862	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	4	32	dep	CM	776:777	arg1	ScCM					817:820	ScCM	817:820	ScCM	817:820	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	4	33	theme	serum-containing	682:697	arg1	SCM					707:709	SCM	707:709	SCM	707:709	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	4	33	theme	serum-containing	682:697	arg1	medium					699:704	serum-containing medium	682:704	serum-containing medium (SCM)	682:710	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	12	34	theme	nanocomposite	1875:1887	arg1	hydrogel					1889:1896	The proposed nanocomposite hydrogel	1862:1896	The proposed nanocomposite hydrogel for MSC-CM delivery	1862:1916	The proposed nanocomposite hydrogel for MSC-CM delivery can open up a new array for its clinical application.
32460846	11	35	theme	sustained	1782:1790	arg1	release					1792:1798	sustained release	1782:1798	sustained release of MSC-CM	1782:1808	CONCLUSION We observed that sustained release of MSC-CM is required to prevent dose-dependent cytotoxicity.
32460846	6	36	theme	degradation	1221:1231	arg1	mix					1214:1216	a mix	1212:1216	a mix of degradation and diffusion kinetics	1212:1254	RESULTS Nanocomposite hydrogel displayed an even distribution of NPs along with high porosity (> 60%) and swelling ratios > 1500%, while its protein release pattern corresponded to a mix of degradation and diffusion kinetics.
32460846	1	37	theme	stromal	104:110	arg1	MSC-CM					137:142	MSC-CM	137:142	MSC-CM	137:142	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM) contains a cocktail of bioactive factors that act synergistically to induce therapeutic effects.
32460846	1	37	theme	stromal	104:110	arg1	medium					129:134	BACKGROUND Mesenchymal stromal cell conditioned medium	81:134	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM)	81:143	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM) contains a cocktail of bioactive factors that act synergistically to induce therapeutic effects.
32460846	6	38	theme	even	1075:1078	arg1	distribution					1080:1091	an even distribution	1072:1091	an even distribution of NPs	1072:1098	RESULTS Nanocomposite hydrogel displayed an even distribution of NPs along with high porosity (> 60%) and swelling ratios > 1500%, while its protein release pattern corresponded to a mix of degradation and diffusion kinetics.
32460846	0	39	theme	delivery	25:32	arg1	system					34:39	hydrogel delivery system	16:39	hydrogel delivery system for mesenchymal stromal cell secretome	16:78	A nanocomposite hydrogel delivery system for mesenchymal stromal cell secretome.
32460846	6	40	theme	release	1180:1186	arg1	pattern					1188:1194	its protein release pattern	1168:1194	its protein release pattern	1168:1194	RESULTS Nanocomposite hydrogel displayed an even distribution of NPs along with high porosity (> 60%) and swelling ratios > 1500%, while its protein release pattern corresponded to a mix of degradation and diffusion kinetics.
32460846	1	41	theme	cell	112:115	arg1	MSC-CM					137:142	MSC-CM	137:142	MSC-CM	137:142	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM) contains a cocktail of bioactive factors that act synergistically to induce therapeutic effects.
32460846	1	41	theme	cell	112:115	arg1	medium					129:134	BACKGROUND Mesenchymal stromal cell conditioned medium	81:134	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM)	81:143	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM) contains a cocktail of bioactive factors that act synergistically to induce therapeutic effects.
32460846	9	42	theme	dose-	1590:1594	arg1	response					1619:1626	a dose- and duration-dependent response	1588:1626	a dose- and duration-dependent response	1588:1626	The cells treated in vitro with 5% and 10% extracts of ScCM-NP-H and SfCM-NP-H exhibited a dose- and duration-dependent response.
32460846	0	43	theme	hydrogel	16:23	arg1	system					34:39	hydrogel delivery system	16:39	hydrogel delivery system for mesenchymal stromal cell secretome	16:78	A nanocomposite hydrogel delivery system for mesenchymal stromal cell secretome.
32460846	4	44	theme	xeno	899:902	arg1	markers					904:910	xeno markers	899:910	xeno markers	899:910	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	1	45	theme	conditioned	117:127	arg1	MSC-CM					137:142	MSC-CM	137:142	MSC-CM	137:142	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM) contains a cocktail of bioactive factors that act synergistically to induce therapeutic effects.
32460846	1	45	theme	conditioned	117:127	arg1	medium					129:134	BACKGROUND Mesenchymal stromal cell conditioned medium	81:134	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM)	81:143	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM) contains a cocktail of bioactive factors that act synergistically to induce therapeutic effects.
32460846	3	46	theme	poly-L-lactide	481:494	arg1	NPs					511:513	NPs	511:513	NPs	511:513	METHODS We developed a nanocomposite-hydrogel (NP-H) comprised of poly-L-lactide nanoparticles (NPs) embedded in gelatin/hyaluronic acid (Gel/HA) hydrogel as a delivery vehicle for MSC-CM.
32460846	3	46	theme	poly-L-lactide	481:494	arg1	nanoparticles					496:508	poly-L-lactide nanoparticles	481:508	poly-L-lactide nanoparticles (NPs) embedded in gelatin/hyaluronic acid (Gel/HA) hydrogel as a delivery vehicle for MSC-CM	481:601	METHODS We developed a nanocomposite-hydrogel (NP-H) comprised of poly-L-lactide nanoparticles (NPs) embedded in gelatin/hyaluronic acid (Gel/HA) hydrogel as a delivery vehicle for MSC-CM.
32460846	7	47	theme	composites	1286:1295	arg1	evaluation					1268:1277	Functional evaluation	1257:1277	Functional evaluation of the composites	1257:1295	Functional evaluation of the composites was determined using MSCs and human fibroblasts (HFFs).
32460846	9	48	theme	%	1540:1540	arg1	extracts					1542:1549	5% and 10% extracts	1531:1549	5% and 10% extracts of ScCM-NP-H and SfCM-NP-H	1531:1576	The cells treated in vitro with 5% and 10% extracts of ScCM-NP-H and SfCM-NP-H exhibited a dose- and duration-dependent response.
32460846	11	49	theme	dose-dependent	1833:1846	arg1	cytotoxicity					1848:1859	dose-dependent cytotoxicity	1833:1859	dose-dependent cytotoxicity	1833:1859	CONCLUSION We observed that sustained release of MSC-CM is required to prevent dose-dependent cytotoxicity.
32460846	6	50	theme	diffusion	1237:1245	arg1	kinetics					1247:1254	diffusion kinetics	1237:1254	diffusion kinetics	1237:1254	RESULTS Nanocomposite hydrogel displayed an even distribution of NPs along with high porosity (> 60%) and swelling ratios > 1500%, while its protein release pattern corresponded to a mix of degradation and diffusion kinetics.
32460846	12	51	theme	new	1932:1934	arg1	array					1936:1940	a new array	1930:1940	a new array for its clinical application	1930:1969	The proposed nanocomposite hydrogel for MSC-CM delivery can open up a new array for its clinical application.
32460846	9	52	theme	SfCM-NP-H	1568:1576	arg1	extracts					1542:1549	5% and 10% extracts	1531:1549	5% and 10% extracts of ScCM-NP-H and SfCM-NP-H	1531:1576	The cells treated in vitro with 5% and 10% extracts of ScCM-NP-H and SfCM-NP-H exhibited a dose- and duration-dependent response.
32460846	9	53	theme	duration-dependent	1600:1617	arg1	response					1619:1626	a dose- and duration-dependent response	1588:1626	a dose- and duration-dependent response	1588:1626	The cells treated in vitro with 5% and 10% extracts of ScCM-NP-H and SfCM-NP-H exhibited a dose- and duration-dependent response.
32460846	1	54	theme	therapeutic	221:231	arg1	effects					233:239	therapeutic effects	221:239	therapeutic effects	221:239	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM) contains a cocktail of bioactive factors that act synergistically to induce therapeutic effects.
32460846	0	55	theme	stromal	57:63	arg1	secretome					70:78	mesenchymal stromal cell secretome	45:78	mesenchymal stromal cell secretome	45:78	A nanocomposite hydrogel delivery system for mesenchymal stromal cell secretome.
32460846	12	56	theme	MSC-CM	1902:1907	arg1	delivery					1909:1916	MSC-CM delivery	1902:1916	MSC-CM delivery	1902:1916	The proposed nanocomposite hydrogel for MSC-CM delivery can open up a new array for its clinical application.
32460846	6	57	theme	Nanocomposite	1039:1051	arg1	hydrogel					1053:1060	Nanocomposite hydrogel	1039:1060	Nanocomposite hydrogel	1039:1060	RESULTS Nanocomposite hydrogel displayed an even distribution of NPs along with high porosity (> 60%) and swelling ratios > 1500%, while its protein release pattern corresponded to a mix of degradation and diffusion kinetics.
32460846	4	58	theme	serum-free	827:836	arg1	SfCM					858:861	SfCM	858:861	SfCM	858:861	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	4	58	theme	serum-free	827:836	arg1	medium					850:855	serum-free conditioned medium	827:855	serum-free conditioned medium (SfCM)	827:862	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	6	59	theme	high	1111:1114	arg1	porosity					1116:1123	high porosity	1111:1123	high porosity (> 60%)	1111:1131	RESULTS Nanocomposite hydrogel displayed an even distribution of NPs along with high porosity (> 60%) and swelling ratios > 1500%, while its protein release pattern corresponded to a mix of degradation and diffusion kinetics.
32460846	6	59	theme	high	1111:1114	arg1	%					1130:1130	> 60%	1126:1130	> 60%	1126:1130	RESULTS Nanocomposite hydrogel displayed an even distribution of NPs along with high porosity (> 60%) and swelling ratios > 1500%, while its protein release pattern corresponded to a mix of degradation and diffusion kinetics.
32460846	2	60	theme	delivery	348:355	arg1	systems					357:363	controlled delivery systems	337:363	controlled delivery systems	337:363	This has been clearly demonstrated by in vivo applications of MSC-CM, but the establishment of controlled delivery systems is an unmet requirement for clinical translation.
32460846	0	61	theme	mesenchymal	45:55	arg1	secretome					70:78	mesenchymal stromal cell secretome	45:78	mesenchymal stromal cell secretome	45:78	A nanocomposite hydrogel delivery system for mesenchymal stromal cell secretome.
32460846	4	62	theme	conditioned	838:848	arg1	SfCM					858:861	SfCM	858:861	SfCM	858:861	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	4	62	theme	conditioned	838:848	arg1	medium					850:855	serum-free conditioned medium	827:855	serum-free conditioned medium (SfCM)	827:862	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	4	63	link	marrow-derived	656:669	arg1	MSCs					671:674	bone marrow-derived MSCs	651:674	bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM)	651:738	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	7	64	theme	human	1327:1331	arg1	HFFs					1346:1349	HFFs	1346:1349	HFFs	1346:1349	Functional evaluation of the composites was determined using MSCs and human fibroblasts (HFFs).
32460846	7	64	theme	human	1327:1331	arg1	fibroblasts					1333:1343	human fibroblasts	1327:1343	human fibroblasts (HFFs)	1327:1350	Functional evaluation of the composites was determined using MSCs and human fibroblasts (HFFs).
32460846	2	65	theme	controlled	337:346	arg1	systems					357:363	controlled delivery systems	337:363	controlled delivery systems	337:363	This has been clearly demonstrated by in vivo applications of MSC-CM, but the establishment of controlled delivery systems is an unmet requirement for clinical translation.
32460846	8	66	contain	having	1474:1479	arg1	groups					1467:1472	ScCM-NP-H groups	1457:1472	ScCM-NP-H groups having maximum activity	1457:1496	The cells seeded directly onto the composites displayed increasing metabolic activities over time, with ScCM-NP-H groups having maximum activity.
32460846	8	66	contain	having	1474:1479	arg2	activity					1489:1496	maximum activity	1481:1496	maximum activity	1481:1496	The cells seeded directly onto the composites displayed increasing metabolic activities over time, with ScCM-NP-H groups having maximum activity.
32460846	8	67	theme	metabolic	1420:1428	arg1	activities					1430:1439	increasing metabolic activities	1409:1439	increasing metabolic activities	1409:1439	The cells seeded directly onto the composites displayed increasing metabolic activities over time, with ScCM-NP-H groups having maximum activity.
32460846	8	68	theme	increasing	1409:1418	arg1	activities					1430:1439	increasing metabolic activities	1409:1439	increasing metabolic activities	1409:1439	The cells seeded directly onto the composites displayed increasing metabolic activities over time, with ScCM-NP-H groups having maximum activity.
32460846	4	69	theme	conditioned	797:807	arg1	medium					809:814	serum-containing conditioned medium	780:814	serum-containing conditioned medium (ScCM)	780:821	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	4	69	theme	conditioned	797:807	arg1	ScCM					817:820	ScCM	817:820	ScCM	817:820	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	6	70	theme	swelling	1137:1144	arg1	ratios					1146:1151	swelling ratios	1137:1151	swelling ratios	1137:1151	RESULTS Nanocomposite hydrogel displayed an even distribution of NPs along with high porosity (> 60%) and swelling ratios > 1500%, while its protein release pattern corresponded to a mix of degradation and diffusion kinetics.
32460846	1	71	theme	BACKGROUND	81:90	arg1	MSC-CM					137:142	MSC-CM	137:142	MSC-CM	137:142	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM) contains a cocktail of bioactive factors that act synergistically to induce therapeutic effects.
32460846	1	71	theme	BACKGROUND	81:90	arg1	medium					129:134	BACKGROUND Mesenchymal stromal cell conditioned medium	81:134	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM)	81:143	BACKGROUND Mesenchymal stromal cell conditioned medium (MSC-CM) contains a cocktail of bioactive factors that act synergistically to induce therapeutic effects.
32460846	8	72	theme	maximum	1481:1487	arg1	activity					1489:1496	maximum activity	1481:1496	maximum activity	1481:1496	The cells seeded directly onto the composites displayed increasing metabolic activities over time, with ScCM-NP-H groups having maximum activity.
32460846	4	73	theme	serum-containing	780:795	arg1	medium					809:814	serum-containing conditioned medium	780:814	serum-containing conditioned medium (ScCM)	780:821	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	4	73	theme	serum-containing	780:795	arg1	ScCM					817:820	ScCM	817:820	ScCM	817:820	First, we optimized the culture conditions for bone marrow-derived MSCs using serum-containing medium (SCM) and serum-free medium (SFM) and characterized the corresponding CM (serum-containing conditioned medium (ScCM) and serum-free conditioned medium (SfCM), respectively) for its potency and xeno markers.
32460846	3	74	theme	acid	547:550	arg1	hydrogel					561:568	gelatin/hyaluronic acid (Gel/HA) hydrogel	528:568	gelatin/hyaluronic acid (Gel/HA) hydrogel as a delivery vehicle for MSC-CM	528:601	METHODS We developed a nanocomposite-hydrogel (NP-H) comprised of poly-L-lactide nanoparticles (NPs) embedded in gelatin/hyaluronic acid (Gel/HA) hydrogel as a delivery vehicle for MSC-CM.
32460846	3	75	from	hydrogel	561:568	arg1	embedded					516:523	embedded	516:523	embedded	516:523	METHODS We developed a nanocomposite-hydrogel (NP-H) comprised of poly-L-lactide nanoparticles (NPs) embedded in gelatin/hyaluronic acid (Gel/HA) hydrogel as a delivery vehicle for MSC-CM.
32460846	5	76	theme	functional	997:1006	arg1	assays					1008:1013	functional assays	997:1013	functional assays	997:1013	Then we prepared a composite matrix followed by physiochemical characterization and functional assays were performed.
32460846	2	77	theme	unmet	371:375	arg1	requirement					377:387	an unmet requirement	368:387	an unmet requirement for clinical translation	368:412	This has been clearly demonstrated by in vivo applications of MSC-CM, but the establishment of controlled delivery systems is an unmet requirement for clinical translation.
32460846	2	77	theme	unmet	371:375	arg1	establishment					320:332	the establishment	316:332	the establishment of controlled delivery systems	316:363	This has been clearly demonstrated by in vivo applications of MSC-CM, but the establishment of controlled delivery systems is an unmet requirement for clinical translation.
32460846	2	78	theme	clinical	393:400	arg1	translation					402:412	clinical translation	393:412	clinical translation	393:412	This has been clearly demonstrated by in vivo applications of MSC-CM, but the establishment of controlled delivery systems is an unmet requirement for clinical translation.
32460846	11	79	theme	MSC-CM	1803:1808	arg1	release					1792:1798	sustained release	1782:1798	sustained release of MSC-CM	1782:1808	CONCLUSION We observed that sustained release of MSC-CM is required to prevent dose-dependent cytotoxicity.
32460846	0	80	theme	cell	65:68	arg1	secretome					70:78	mesenchymal stromal cell secretome	45:78	mesenchymal stromal cell secretome	45:78	A nanocomposite hydrogel delivery system for mesenchymal stromal cell secretome.
32460846	6	81	theme	protein	1172:1178	arg1	pattern					1188:1194	its protein release pattern	1168:1194	its protein release pattern	1168:1194	RESULTS Nanocomposite hydrogel displayed an even distribution of NPs along with high porosity (> 60%) and swelling ratios > 1500%, while its protein release pattern corresponded to a mix of degradation and diffusion kinetics.
32460846	2	82	dep	in	280:281	arg1	vivo					283:286	vivo	283:286	vivo	283:286	This has been clearly demonstrated by in vivo applications of MSC-CM, but the establishment of controlled delivery systems is an unmet requirement for clinical translation.
32460846	10	83	theme	%	1691:1691	arg1	ScCM-NP-H					1693:1701	10% ScCM-NP-H	1689:1701	10% ScCM-NP-H	1689:1701	Cell activities reduced considerably for all groups, except 10% ScCM-NP-H, which displayed a significant increase over time.
33171184	8	0	theme	adsorption	1297:1306	arg1	sites					1315:1319	the adsorption energy sites	1293:1319	the adsorption energy sites obtained with isopropanol	1293:1345	The distribution function of the adsorption energy sites obtained with isopropanol revealed the decrease in the number of the high energy sites with increase of CS/Bt mass ratio.
33171184	4	1	theme	CS	717:718	arg1	effect					707:712	the effect	703:712	the effect of CS on dispersive component of the surface energy of the bentonite	703:781	IGC at infinite dilution (IGC-ID) was used to understand the effect of CS on dispersive component of the surface energy of the bentonite.
33171184	8	2	theme	energy	1308:1313	arg1	sites					1315:1319	the adsorption energy sites	1293:1319	the adsorption energy sites obtained with isopropanol	1293:1345	The distribution function of the adsorption energy sites obtained with isopropanol revealed the decrease in the number of the high energy sites with increase of CS/Bt mass ratio.
33171184	3	3	theme	inverse	525:531	arg1	IGC					553:555	IGC	553:555	IGC	553:555	In this study, inverse gas chromatography (IGC) was implemented to characterize physico-chemical properties of CSBt composites surface.
33171184	3	3	theme	inverse	525:531	arg1	chromatography					537:550	inverse gas chromatography	525:550	inverse gas chromatography (IGC)	525:556	In this study, inverse gas chromatography (IGC) was implemented to characterize physico-chemical properties of CSBt composites surface.
33171184	8	4	from	decrease	1360:1367	arg1	number					1376:1381	the number	1372:1381	the number of the high energy sites with increase of CS/Bt mass ratio	1372:1440	The distribution function of the adsorption energy sites obtained with isopropanol revealed the decrease in the number of the high energy sites with increase of CS/Bt mass ratio.
33171184	0	5	theme	gas	89:91	arg1	chromatography					93:106	inverse gas chromatography	81:106	inverse gas chromatography	81:106	Characterization of surface properties of chitosan/bentonite composites beads by inverse gas chromatography.
33171184	6	6	theme	finite	901:906	arg1	IGC-FC					923:928	IGC-FC	923:928	IGC-FC	923:928	The IGC at finite concentration (IGC-FC) was also implemented allowing us to reach several parameters such as: specific surface area with organic probes and the distribution functions of the adsorption energy sites on the solid surface.
33171184	6	6	theme	finite	901:906	arg1	concentration					908:920	finite concentration	901:920	finite concentration (IGC-FC)	901:929	The IGC at finite concentration (IGC-FC) was also implemented allowing us to reach several parameters such as: specific surface area with organic probes and the distribution functions of the adsorption energy sites on the solid surface.
33171184	2	7	dep	composites	484:493	arg1	beads					495:499	beads	495:499	beads	495:499	Fourier Transform Infrared Spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, XPS and Brunauer-Emmett-Teller BET analysis have been used to provide new insights on the composition and morphology of CSBt composites beads surface.
33171184	2	8	theme	BET	390:392	arg1	analysis					394:401	Brunauer-Emmett-Teller BET analysis	367:401	Brunauer-Emmett-Teller BET analysis	367:401	Fourier Transform Infrared Spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, XPS and Brunauer-Emmett-Teller BET analysis have been used to provide new insights on the composition and morphology of CSBt composites beads surface.
33171184	4	9	from	effect	707:712	arg1	component					734:742	dispersive component	723:742	dispersive component of the surface energy of the bentonite	723:781	IGC at infinite dilution (IGC-ID) was used to understand the effect of CS on dispersive component of the surface energy of the bentonite.
33171184	8	10	theme	high	1390:1393	arg1	sites					1402:1406	the high energy sites	1386:1406	the high energy sites with increase of CS/Bt mass ratio	1386:1440	The distribution function of the adsorption energy sites obtained with isopropanol revealed the decrease in the number of the high energy sites with increase of CS/Bt mass ratio.
33171184	4	11	theme	bentonite	773:781	arg1	energy					759:764	the surface energy	747:764	the surface energy of the bentonite	747:781	IGC at infinite dilution (IGC-ID) was used to understand the effect of CS on dispersive component of the surface energy of the bentonite.
33171184	4	12	theme	energy	759:764	arg1	component					734:742	dispersive component	723:742	dispersive component of the surface energy of the bentonite	723:781	IGC at infinite dilution (IGC-ID) was used to understand the effect of CS on dispersive component of the surface energy of the bentonite.
33171184	6	13	theme	several	973:979	arg1	parameters					981:990	several parameters	973:990	several parameters such as: specific surface area with organic probes	973:1041	The IGC at finite concentration (IGC-FC) was also implemented allowing us to reach several parameters such as: specific surface area with organic probes and the distribution functions of the adsorption energy sites on the solid surface.
33171184	4	14	theme	surface	751:757	arg1	energy					759:764	the surface energy	747:764	the surface energy of the bentonite	747:781	IGC at infinite dilution (IGC-ID) was used to understand the effect of CS on dispersive component of the surface energy of the bentonite.
33171184	8	15	theme	sites	1315:1319	arg1	function					1281:1288	The distribution function	1264:1288	The distribution function of the adsorption energy sites obtained with isopropanol	1264:1345	The distribution function of the adsorption energy sites obtained with isopropanol revealed the decrease in the number of the high energy sites with increase of CS/Bt mass ratio.
33171184	6	16	from	functions	1064:1072	arg1	surface					1118:1124	the solid surface	1108:1124	the solid surface	1108:1124	The IGC at finite concentration (IGC-FC) was also implemented allowing us to reach several parameters such as: specific surface area with organic probes and the distribution functions of the adsorption energy sites on the solid surface.
33171184	2	17	theme	diffraction	303:313	arg1	analysis					315:322	X-ray diffraction analysis	297:322	X-ray diffraction analysis	297:322	Fourier Transform Infrared Spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, XPS and Brunauer-Emmett-Teller BET analysis have been used to provide new insights on the composition and morphology of CSBt composites beads surface.
33171184	8	18	theme	distribution	1268:1279	arg1	function					1281:1288	The distribution function	1264:1288	The distribution function of the adsorption energy sites obtained with isopropanol	1264:1345	The distribution function of the adsorption energy sites obtained with isopropanol revealed the decrease in the number of the high energy sites with increase of CS/Bt mass ratio.
33171184	7	19	theme	significant	1150:1160	arg1	decreases					1162:1170	the most significant decreases	1141:1170	the most significant decreases	1141:1170	In this case, the most significant decreases were observed in the specific surface area obtained with the octane and isopropanol probes.
33171184	4	20	theme	dispersive	723:732	arg1	component					734:742	dispersive component	723:742	dispersive component of the surface energy of the bentonite	723:781	IGC at infinite dilution (IGC-ID) was used to understand the effect of CS on dispersive component of the surface energy of the bentonite.
33171184	6	21	theme	organic	1028:1034	arg1	probes					1036:1041	organic probes	1028:1041	organic probes	1028:1041	The IGC at finite concentration (IGC-FC) was also implemented allowing us to reach several parameters such as: specific surface area with organic probes and the distribution functions of the adsorption energy sites on the solid surface.
33171184	2	22	theme	X-ray	297:301	arg1	analysis					315:322	X-ray diffraction analysis	297:322	X-ray diffraction analysis	297:322	Fourier Transform Infrared Spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, XPS and Brunauer-Emmett-Teller BET analysis have been used to provide new insights on the composition and morphology of CSBt composites beads surface.
33171184	2	23	theme	new	429:431	arg1	insights					433:440	new insights	429:440	new insights on the composition and morphology of CSBt composites beads surface	429:507	Fourier Transform Infrared Spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, XPS and Brunauer-Emmett-Teller BET analysis have been used to provide new insights on the composition and morphology of CSBt composites beads surface.
33171184	2	24	theme	composites	484:493	arg1	morphology					465:474	morphology	465:474	morphology	465:474	Fourier Transform Infrared Spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, XPS and Brunauer-Emmett-Teller BET analysis have been used to provide new insights on the composition and morphology of CSBt composites beads surface.
33171184	2	24	theme	composites	484:493	arg1	composition					449:459	composition	449:459	composition	449:459	Fourier Transform Infrared Spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, XPS and Brunauer-Emmett-Teller BET analysis have been used to provide new insights on the composition and morphology of CSBt composites beads surface.
33171184	5	25	theme	increasing	788:797	arg1	CS					809:810	CS	809:810	CS	809:810	The increasing amount of CS leads to significantly decrease of Bt γsd emphasizing the Bt coating with CS.
33171184	5	25	theme	increasing	788:797	arg1	amount					799:804	The increasing amount	784:804	The increasing amount of CS	784:810	The increasing amount of CS leads to significantly decrease of Bt γsd emphasizing the Bt coating with CS.
33171184	6	26	theme	specific	1001:1008	arg1	area					1018:1021	specific surface area	1001:1021	specific surface area with organic probes	1001:1041	The IGC at finite concentration (IGC-FC) was also implemented allowing us to reach several parameters such as: specific surface area with organic probes and the distribution functions of the adsorption energy sites on the solid surface.
33171184	1	27	theme	solution	183:190	arg1	dropwise					169:176	dropwise	169:176	dropwise of a solution containing chitosan and bentonite to an alkaline NaOH solution	169:253	Chitosan/bentonite (CSBt) composites beads were prepared by dropwise of a solution containing chitosan and bentonite to an alkaline NaOH solution.
33171184	2	28	theme	CSBt	479:482	arg1	composites					484:493	CSBt composites	479:493	CSBt composites beads surface	479:507	Fourier Transform Infrared Spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, XPS and Brunauer-Emmett-Teller BET analysis have been used to provide new insights on the composition and morphology of CSBt composites beads surface.
33171184	6	29	from	concentration	908:920	arg1	IGC					894:896	The IGC	890:896	The IGC at finite concentration (IGC-FC)	890:929	The IGC at finite concentration (IGC-FC) was also implemented allowing us to reach several parameters such as: specific surface area with organic probes and the distribution functions of the adsorption energy sites on the solid surface.
33171184	7	30	theme	specific	1193:1200	arg1	area					1210:1213	the specific surface area	1189:1213	the specific surface area obtained with the octane and isopropanol probes	1189:1261	In this case, the most significant decreases were observed in the specific surface area obtained with the octane and isopropanol probes.
33171184	3	31	theme	physico-chemical	590:605	arg1	properties					607:616	physico-chemical properties	590:616	physico-chemical properties of CSBt composites surface	590:643	In this study, inverse gas chromatography (IGC) was implemented to characterize physico-chemical properties of CSBt composites surface.
33171184	7	32	theme	most	1145:1148	arg1	decreases					1162:1170	the most significant decreases	1141:1170	the most significant decreases	1141:1170	In this case, the most significant decreases were observed in the specific surface area obtained with the octane and isopropanol probes.
33171184	6	33	from	parameters	981:990	arg1	surface					1118:1124	the solid surface	1108:1124	the solid surface	1108:1124	The IGC at finite concentration (IGC-FC) was also implemented allowing us to reach several parameters such as: specific surface area with organic probes and the distribution functions of the adsorption energy sites on the solid surface.
33171184	2	34	theme	photoelectron	331:343	arg1	spectroscopy					345:356	X-ray photoelectron spectroscopy	325:356	X-ray photoelectron spectroscopy	325:356	Fourier Transform Infrared Spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, XPS and Brunauer-Emmett-Teller BET analysis have been used to provide new insights on the composition and morphology of CSBt composites beads surface.
33171184	0	35	theme	properties	28:37	arg1	Characterization					0:15	Characterization	0:15	Characterization of surface properties of chitosan/bentonite composites	0:70	Characterization of surface properties of chitosan/bentonite composites beads by inverse gas chromatography.
33171184	6	36	theme	distribution	1051:1062	arg1	functions					1064:1072	the distribution functions	1047:1072	the distribution functions of the adsorption energy sites on the solid surface	1047:1124	The IGC at finite concentration (IGC-FC) was also implemented allowing us to reach several parameters such as: specific surface area with organic probes and the distribution functions of the adsorption energy sites on the solid surface.
33171184	8	37	theme	CS/Bt	1425:1429	arg1	ratio					1436:1440	CS/Bt mass ratio	1425:1440	CS/Bt mass ratio	1425:1440	The distribution function of the adsorption energy sites obtained with isopropanol revealed the decrease in the number of the high energy sites with increase of CS/Bt mass ratio.
33171184	2	38	theme	X-ray	325:329	arg1	spectroscopy					345:356	X-ray photoelectron spectroscopy	325:356	X-ray photoelectron spectroscopy	325:356	Fourier Transform Infrared Spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, XPS and Brunauer-Emmett-Teller BET analysis have been used to provide new insights on the composition and morphology of CSBt composites beads surface.
33171184	6	39	from	surface	1118:1124	arg1	parameters					981:990	several parameters	973:990	several parameters such as: specific surface area with organic probes	973:1041	The IGC at finite concentration (IGC-FC) was also implemented allowing us to reach several parameters such as: specific surface area with organic probes and the distribution functions of the adsorption energy sites on the solid surface.
33171184	6	39	from	surface	1118:1124	arg1	functions					1064:1072	the distribution functions	1047:1072	the distribution functions of the adsorption energy sites on the solid surface	1047:1124	The IGC at finite concentration (IGC-FC) was also implemented allowing us to reach several parameters such as: specific surface area with organic probes and the distribution functions of the adsorption energy sites on the solid surface.
33171184	1	40	theme	Chitosan/bentonite	109:126	arg1	beads					146:150	Chitosan/bentonite (CSBt) composites beads	109:150	Chitosan/bentonite (CSBt) composites beads	109:150	Chitosan/bentonite (CSBt) composites beads were prepared by dropwise of a solution containing chitosan and bentonite to an alkaline NaOH solution.
33171184	1	41	theme	CSBt	129:132	arg1	beads					146:150	Chitosan/bentonite (CSBt) composites beads	109:150	Chitosan/bentonite (CSBt) composites beads	109:150	Chitosan/bentonite (CSBt) composites beads were prepared by dropwise of a solution containing chitosan and bentonite to an alkaline NaOH solution.
33171184	5	42	theme	Bt	847:848	arg1	γsd					850:852	Bt γsd	847:852	Bt γsd emphasizing the Bt coating with CS	847:887	The increasing amount of CS leads to significantly decrease of Bt γsd emphasizing the Bt coating with CS.
33171184	0	43	theme	chitosan/bentonite	42:59	arg1	composites					61:70	chitosan/bentonite composites	42:70	chitosan/bentonite composites	42:70	Characterization of surface properties of chitosan/bentonite composites beads by inverse gas chromatography.
33171184	1	44	contain	containing	192:201	arg2	bentonite					216:224	bentonite	216:224	bentonite	216:224	Chitosan/bentonite (CSBt) composites beads were prepared by dropwise of a solution containing chitosan and bentonite to an alkaline NaOH solution.
33171184	1	44	contain	containing	192:201	arg2	chitosan					203:210	chitosan	203:210	chitosan	203:210	Chitosan/bentonite (CSBt) composites beads were prepared by dropwise of a solution containing chitosan and bentonite to an alkaline NaOH solution.
33171184	1	44	contain	containing	192:201	arg1	solution					183:190	a solution	181:190	a solution containing chitosan and bentonite to an alkaline NaOH solution	181:253	Chitosan/bentonite (CSBt) composites beads were prepared by dropwise of a solution containing chitosan and bentonite to an alkaline NaOH solution.
33171184	3	45	theme	composites	626:635	arg1	surface					637:643	CSBt composites surface	621:643	CSBt composites surface	621:643	In this study, inverse gas chromatography (IGC) was implemented to characterize physico-chemical properties of CSBt composites surface.
33171184	4	46	from	dilution	662:669	arg1	IGC					646:648	IGC	646:648	IGC at infinite dilution (IGC-ID)	646:678	IGC at infinite dilution (IGC-ID) was used to understand the effect of CS on dispersive component of the surface energy of the bentonite.
33171184	5	47	theme	γsd	850:852	arg1	decrease					835:842	significantly decrease	821:842	significantly decrease of Bt γsd emphasizing the Bt coating with CS	821:887	The increasing amount of CS leads to significantly decrease of Bt γsd emphasizing the Bt coating with CS.
33171184	6	48	theme	surface	1010:1016	arg1	area					1018:1021	specific surface area	1001:1021	specific surface area with organic probes	1001:1041	The IGC at finite concentration (IGC-FC) was also implemented allowing us to reach several parameters such as: specific surface area with organic probes and the distribution functions of the adsorption energy sites on the solid surface.
33171184	8	49	theme	mass	1431:1434	arg1	ratio					1436:1440	CS/Bt mass ratio	1425:1440	CS/Bt mass ratio	1425:1440	The distribution function of the adsorption energy sites obtained with isopropanol revealed the decrease in the number of the high energy sites with increase of CS/Bt mass ratio.
33171184	3	50	theme	surface	637:643	arg1	properties					607:616	physico-chemical properties	590:616	physico-chemical properties of CSBt composites surface	590:643	In this study, inverse gas chromatography (IGC) was implemented to characterize physico-chemical properties of CSBt composites surface.
33171184	7	51	located	observed	1177:1184	arg1	area					1210:1213	the specific surface area	1189:1213	the specific surface area obtained with the octane and isopropanol probes	1189:1261	In this case, the most significant decreases were observed in the specific surface area obtained with the octane and isopropanol probes.
33171184	7	51	located	observed	1177:1184	arg2	decreases					1162:1170	the most significant decreases	1141:1170	the most significant decreases	1141:1170	In this case, the most significant decreases were observed in the specific surface area obtained with the octane and isopropanol probes.
33171184	7	51	located	observed	1177:1184	arg1	case					1135:1138	this case	1130:1138	this case	1130:1138	In this case, the most significant decreases were observed in the specific surface area obtained with the octane and isopropanol probes.
33171184	8	52	with	sites	1402:1406	arg1	increase					1413:1420	increase	1413:1420	increase of CS/Bt mass ratio	1413:1440	The distribution function of the adsorption energy sites obtained with isopropanol revealed the decrease in the number of the high energy sites with increase of CS/Bt mass ratio.
33171184	5	53	theme	CS	809:810	arg1	CS					809:810	CS	809:810	CS	809:810	The increasing amount of CS leads to significantly decrease of Bt γsd emphasizing the Bt coating with CS.
33171184	5	53	theme	CS	809:810	arg1	amount					799:804	The increasing amount	784:804	The increasing amount of CS	784:810	The increasing amount of CS leads to significantly decrease of Bt γsd emphasizing the Bt coating with CS.
33171184	7	54	theme	isopropanol	1244:1254	arg1	probes					1256:1261	the octane and isopropanol probes	1229:1261	probes	1256:1261	In this case, the most significant decreases were observed in the specific surface area obtained with the octane and isopropanol probes.
33171184	2	55	dep	composition	449:459	arg1	the					445:447	the	445:447	the	445:447	Fourier Transform Infrared Spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, XPS and Brunauer-Emmett-Teller BET analysis have been used to provide new insights on the composition and morphology of CSBt composites beads surface.
33171184	6	56	from	sites	1099:1103	arg1	surface					1118:1124	the solid surface	1108:1124	the solid surface	1108:1124	The IGC at finite concentration (IGC-FC) was also implemented allowing us to reach several parameters such as: specific surface area with organic probes and the distribution functions of the adsorption energy sites on the solid surface.
33171184	2	57	theme	Brunauer-Emmett-Teller	367:388	arg1	analysis					394:401	Brunauer-Emmett-Teller BET analysis	367:401	Brunauer-Emmett-Teller BET analysis	367:401	Fourier Transform Infrared Spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, XPS and Brunauer-Emmett-Teller BET analysis have been used to provide new insights on the composition and morphology of CSBt composites beads surface.
33171184	0	58	theme	composites	61:70	arg1	properties					28:37	surface properties	20:37	surface properties of chitosan/bentonite composites	20:70	Characterization of surface properties of chitosan/bentonite composites beads by inverse gas chromatography.
33171184	8	59	theme	ratio	1436:1440	arg1	increase					1413:1420	increase	1413:1420	increase of CS/Bt mass ratio	1413:1440	The distribution function of the adsorption energy sites obtained with isopropanol revealed the decrease in the number of the high energy sites with increase of CS/Bt mass ratio.
33171184	3	60	theme	gas	533:535	arg1	IGC					553:555	IGC	553:555	IGC	553:555	In this study, inverse gas chromatography (IGC) was implemented to characterize physico-chemical properties of CSBt composites surface.
33171184	3	60	theme	gas	533:535	arg1	chromatography					537:550	inverse gas chromatography	525:550	inverse gas chromatography (IGC)	525:556	In this study, inverse gas chromatography (IGC) was implemented to characterize physico-chemical properties of CSBt composites surface.
33171184	1	61	theme	composites	135:144	arg1	beads					146:150	Chitosan/bentonite (CSBt) composites beads	109:150	Chitosan/bentonite (CSBt) composites beads	109:150	Chitosan/bentonite (CSBt) composites beads were prepared by dropwise of a solution containing chitosan and bentonite to an alkaline NaOH solution.
33171184	8	62	theme	sites	1402:1406	arg1	number					1376:1381	the number	1372:1381	the number of the high energy sites with increase of CS/Bt mass ratio	1372:1440	The distribution function of the adsorption energy sites obtained with isopropanol revealed the decrease in the number of the high energy sites with increase of CS/Bt mass ratio.
33171184	6	63	with	area	1018:1021	arg1	probes					1036:1041	organic probes	1028:1041	organic probes	1028:1041	The IGC at finite concentration (IGC-FC) was also implemented allowing us to reach several parameters such as: specific surface area with organic probes and the distribution functions of the adsorption energy sites on the solid surface.
33171184	8	64	theme	energy	1395:1400	arg1	sites					1402:1406	the high energy sites	1386:1406	the high energy sites with increase of CS/Bt mass ratio	1386:1440	The distribution function of the adsorption energy sites obtained with isopropanol revealed the decrease in the number of the high energy sites with increase of CS/Bt mass ratio.
33171184	4	65	used	used	684:687	arg2	dilution					662:669	infinite dilution	653:669	infinite dilution (IGC-ID)	653:678	IGC at infinite dilution (IGC-ID) was used to understand the effect of CS on dispersive component of the surface energy of the bentonite.
33171184	4	65	used	used	684:687	arg2	IGC					646:648	IGC	646:648	IGC at infinite dilution (IGC-ID)	646:678	IGC at infinite dilution (IGC-ID) was used to understand the effect of CS on dispersive component of the surface energy of the bentonite.
33171184	4	65	used	used	684:687	arg2	IGC-ID					672:677	IGC-ID	672:677	IGC-ID	672:677	IGC at infinite dilution (IGC-ID) was used to understand the effect of CS on dispersive component of the surface energy of the bentonite.
33171184	7	66	theme	octane	1233:1238	arg1	probes					1256:1261	the octane and isopropanol probes	1229:1261	probes	1256:1261	In this case, the most significant decreases were observed in the specific surface area obtained with the octane and isopropanol probes.
33171184	1	67	theme	alkaline	232:239	arg1	solution					246:253	an alkaline NaOH solution	229:253	an alkaline NaOH solution	229:253	Chitosan/bentonite (CSBt) composites beads were prepared by dropwise of a solution containing chitosan and bentonite to an alkaline NaOH solution.
33171184	2	68	theme	Infrared	274:281	arg1	Spectroscopy					283:294	Infrared Spectroscopy	274:294	Infrared Spectroscopy	274:294	Fourier Transform Infrared Spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, XPS and Brunauer-Emmett-Teller BET analysis have been used to provide new insights on the composition and morphology of CSBt composites beads surface.
33171184	2	69	used	used	413:416	arg2	Fourier					256:262	Fourier	256:262	Fourier Transform Infrared Spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, XPS and Brunauer-Emmett-Teller BET analysis	256:401	Fourier Transform Infrared Spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, XPS and Brunauer-Emmett-Teller BET analysis have been used to provide new insights on the composition and morphology of CSBt composites beads surface.
33171184	6	70	theme	sites	1099:1103	arg1	parameters					981:990	several parameters	973:990	several parameters such as: specific surface area with organic probes	973:1041	The IGC at finite concentration (IGC-FC) was also implemented allowing us to reach several parameters such as: specific surface area with organic probes and the distribution functions of the adsorption energy sites on the solid surface.
33171184	6	70	theme	sites	1099:1103	arg1	functions					1064:1072	the distribution functions	1047:1072	the distribution functions of the adsorption energy sites on the solid surface	1047:1124	The IGC at finite concentration (IGC-FC) was also implemented allowing us to reach several parameters such as: specific surface area with organic probes and the distribution functions of the adsorption energy sites on the solid surface.
33171184	6	71	theme	solid	1112:1116	arg1	surface					1118:1124	the solid surface	1108:1124	the solid surface	1108:1124	The IGC at finite concentration (IGC-FC) was also implemented allowing us to reach several parameters such as: specific surface area with organic probes and the distribution functions of the adsorption energy sites on the solid surface.
33171184	1	72	theme	NaOH	241:244	arg1	solution					246:253	an alkaline NaOH solution	229:253	an alkaline NaOH solution	229:253	Chitosan/bentonite (CSBt) composites beads were prepared by dropwise of a solution containing chitosan and bentonite to an alkaline NaOH solution.
33171184	0	73	theme	inverse	81:87	arg1	chromatography					93:106	inverse gas chromatography	81:106	inverse gas chromatography	81:106	Characterization of surface properties of chitosan/bentonite composites beads by inverse gas chromatography.
33171184	2	74	from	insights	433:440	arg1	morphology					465:474	morphology	465:474	morphology	465:474	Fourier Transform Infrared Spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, XPS and Brunauer-Emmett-Teller BET analysis have been used to provide new insights on the composition and morphology of CSBt composites beads surface.
33171184	2	74	from	insights	433:440	arg1	composition					449:459	composition	449:459	composition	449:459	Fourier Transform Infrared Spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, XPS and Brunauer-Emmett-Teller BET analysis have been used to provide new insights on the composition and morphology of CSBt composites beads surface.
33171184	6	75	theme	energy	1092:1097	arg1	sites					1099:1103	the adsorption energy sites	1077:1103	the adsorption energy sites on the solid surface	1077:1124	The IGC at finite concentration (IGC-FC) was also implemented allowing us to reach several parameters such as: specific surface area with organic probes and the distribution functions of the adsorption energy sites on the solid surface.
33171184	2	76	dep	Fourier	256:262	arg1	Transform					264:272	Transform	264:272	Transform Infrared Spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, XPS and Brunauer-Emmett-Teller BET analysis	264:401	Fourier Transform Infrared Spectroscopy, X-ray diffraction analysis, X-ray photoelectron spectroscopy, XPS and Brunauer-Emmett-Teller BET analysis have been used to provide new insights on the composition and morphology of CSBt composites beads surface.
33171184	4	77	theme	infinite	653:660	arg1	IGC-ID					672:677	IGC-ID	672:677	IGC-ID	672:677	IGC at infinite dilution (IGC-ID) was used to understand the effect of CS on dispersive component of the surface energy of the bentonite.
33171184	4	77	theme	infinite	653:660	arg1	dilution					662:669	infinite dilution	653:669	infinite dilution (IGC-ID)	653:678	IGC at infinite dilution (IGC-ID) was used to understand the effect of CS on dispersive component of the surface energy of the bentonite.
33171184	7	78	theme	surface	1202:1208	arg1	area					1210:1213	the specific surface area	1189:1213	the specific surface area obtained with the octane and isopropanol probes	1189:1261	In this case, the most significant decreases were observed in the specific surface area obtained with the octane and isopropanol probes.
33171184	0	79	theme	surface	20:26	arg1	properties					28:37	surface properties	20:37	surface properties of chitosan/bentonite composites	20:70	Characterization of surface properties of chitosan/bentonite composites beads by inverse gas chromatography.
33171184	6	80	theme	adsorption	1081:1090	arg1	sites					1099:1103	the adsorption energy sites	1077:1103	the adsorption energy sites on the solid surface	1077:1124	The IGC at finite concentration (IGC-FC) was also implemented allowing us to reach several parameters such as: specific surface area with organic probes and the distribution functions of the adsorption energy sites on the solid surface.
32526303	6	0	theme	polarization	947:958	arg1	measurements					960:971	Potentiodynamic polarization measurements	931:971	Potentiodynamic polarization measurements	931:971	Potentiodynamic polarization measurements confirmed that cellulose based inhibitors act as a mixed type inhibitor.
32526303	7	1	theme	cellulose-niacin	1081:1096	arg1	composite					1098:1106	ethyl cellulose-niacin composite	1075:1106	ethyl cellulose-niacin composite (NEC)	1075:1112	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	7	1	theme	cellulose-niacin	1081:1096	arg1	NEC					1109:1111	NEC	1109:1111	NEC	1109:1111	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	6	2	theme	mixed	1024:1028	arg1	inhibitor					1035:1043	a mixed type inhibitor	1022:1043	a mixed type inhibitor	1022:1043	Potentiodynamic polarization measurements confirmed that cellulose based inhibitors act as a mixed type inhibitor.
32526303	6	2	theme	mixed	1024:1028	arg1	inhibitors					1004:1013	cellulose based inhibitors	988:1013	cellulose based inhibitors	988:1013	Potentiodynamic polarization measurements confirmed that cellulose based inhibitors act as a mixed type inhibitor.
32526303	4	3	theme	spectroscopy	810:821	arg1	techniques					829:838	polarization and electrochemical impedance spectroscopy (EIS) techniques	767:838	polarization and electrochemical impedance spectroscopy (EIS) techniques	767:838	The anticorrosive performance of cellulose composites for copper in 3.5% NaCl solutions were evaluated using polarization and electrochemical impedance spectroscopy (EIS) techniques.
32526303	7	4	theme	83.4	1268:1271	arg1	%					1272:1272	33.2 and 83.4%	1259:1272	%	1272:1272	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	6	5	theme	Potentiodynamic	931:945	arg1	measurements					960:971	Potentiodynamic polarization measurements	931:971	Potentiodynamic polarization measurements	931:971	Potentiodynamic polarization measurements confirmed that cellulose based inhibitors act as a mixed type inhibitor.
32526303	2	6	theme	cellulosic	384:393	arg1	materials					395:403	different cellulosic materials	374:403	different cellulosic materials	374:403	Therefore, novel benign high performance corrosion inhibitors based on biopolymer were synthesized in-situ using different cellulosic materials and niacin.
32526303	4	7	theme	electrochemical	784:798	arg1	EIS					824:826	EIS	824:826	EIS	824:826	The anticorrosive performance of cellulose composites for copper in 3.5% NaCl solutions were evaluated using polarization and electrochemical impedance spectroscopy (EIS) techniques.
32526303	4	7	theme	electrochemical	784:798	arg1	spectroscopy					810:821	electrochemical impedance spectroscopy	784:821	electrochemical impedance spectroscopy (EIS)	784:827	The anticorrosive performance of cellulose composites for copper in 3.5% NaCl solutions were evaluated using polarization and electrochemical impedance spectroscopy (EIS) techniques.
32526303	3	8	theme	dispersive	634:643	arg1	EDX					652:654	EDX	652:654	EDX	652:654	Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX).
32526303	3	8	theme	dispersive	634:643	arg1	X-ray					645:649	energy dispersive X-ray	627:649	energy dispersive X-ray (EDX)	627:655	Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX).
32526303	7	9	theme	microcrystalline	1145:1160	arg1	NMCC					1190:1193	NMCC	1190:1193	NMCC	1190:1193	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	7	9	theme	microcrystalline	1145:1160	arg1	composite					1179:1187	microcrystalline cellulose-niacin composite	1145:1187	microcrystalline cellulose-niacin composite (NMCC)	1145:1194	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	3	10	dep	Fourier	485:491	arg1	transform					493:501	transform	493:501	transform infrared (FTIR)	493:517	Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX).
32526303	4	11	theme	polarization	767:778	arg1	techniques					829:838	polarization and electrochemical impedance spectroscopy (EIS) techniques	767:838	polarization and electrochemical impedance spectroscopy (EIS) techniques	767:838	The anticorrosive performance of cellulose composites for copper in 3.5% NaCl solutions were evaluated using polarization and electrochemical impedance spectroscopy (EIS) techniques.
32526303	6	12	theme	type	1030:1033	arg1	inhibitor					1035:1043	a mixed type inhibitor	1022:1043	a mixed type inhibitor	1022:1043	Potentiodynamic polarization measurements confirmed that cellulose based inhibitors act as a mixed type inhibitor.
32526303	6	12	theme	type	1030:1033	arg1	inhibitors					1004:1013	cellulose based inhibitors	988:1013	cellulose based inhibitors	988:1013	Potentiodynamic polarization measurements confirmed that cellulose based inhibitors act as a mixed type inhibitor.
32526303	0	13	theme	copper	102:107	arg1	corrosion					89:97	corrosion	89:97	corrosion of copper in sodium chloride solutions	89:136	Environmentally benign corrosion inhibitors based on cellulose niacin nano-composite for corrosion of copper in sodium chloride solutions.
32526303	7	14	theme	cellulose-niacin	1162:1177	arg1	NMCC					1190:1193	NMCC	1190:1193	NMCC	1190:1193	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	7	14	theme	cellulose-niacin	1162:1177	arg1	composite					1179:1187	microcrystalline cellulose-niacin composite	1145:1187	microcrystalline cellulose-niacin composite (NMCC)	1145:1194	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	2	15	theme	corrosion	302:310	arg1	inhibitors					312:321	novel benign high performance corrosion inhibitors	272:321	novel benign high performance corrosion inhibitors based on biopolymer	272:341	Therefore, novel benign high performance corrosion inhibitors based on biopolymer were synthesized in-situ using different cellulosic materials and niacin.
32526303	4	16	theme	3.5	726:728	arg1	%					729:729	%	729:729	%	729:729	The anticorrosive performance of cellulose composites for copper in 3.5% NaCl solutions were evaluated using polarization and electrochemical impedance spectroscopy (EIS) techniques.
32526303	0	17	theme	chloride	119:126	arg1	solutions					128:136	sodium chloride solutions	112:136	sodium chloride solutions	112:136	Environmentally benign corrosion inhibitors based on cellulose niacin nano-composite for corrosion of copper in sodium chloride solutions.
32526303	3	18	theme	gravimetric	528:538	arg1	TGA					550:552	TGA	550:552	TGA	550:552	Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX).
32526303	3	18	theme	gravimetric	528:538	arg1	analysis					540:547	thermal gravimetric analysis	520:547	thermal gravimetric analysis (TGA)	520:553	Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX).
32526303	5	19	theme	composites	889:898	arg1	morphology					849:858	Surface morphology	841:858	Surface morphology of uninhibited and inhibited composites	841:898	Surface morphology of uninhibited and inhibited composites was studied using SEM and EDX.
32526303	2	20	theme	performance	290:300	arg1	inhibitors					312:321	novel benign high performance corrosion inhibitors	272:321	novel benign high performance corrosion inhibitors based on biopolymer	272:341	Therefore, novel benign high performance corrosion inhibitors based on biopolymer were synthesized in-situ using different cellulosic materials and niacin.
32526303	1	21	theme	high	166:169	arg1	performance					171:181	high performance	166:181	high performance	166:181	There is a high demand for high performance, effective and eco-friendly corrosion inhibitors for industrial applications.
32526303	0	22	theme	sodium	112:117	arg1	solutions					128:136	sodium chloride solutions	112:136	sodium chloride solutions	112:136	Environmentally benign corrosion inhibitors based on cellulose niacin nano-composite for corrosion of copper in sodium chloride solutions.
32526303	3	23	dep	infrared	503:510	arg1	FTIR					513:516	FTIR	513:516	FTIR	513:516	Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX).
32526303	7	24	theme	NaCl	1338:1341	arg1	solutions					1343:1351	3.5% NaCl solutions	1333:1351	3.5% NaCl solutions	1333:1351	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	2	25	theme	high	285:288	arg1	inhibitors					312:321	novel benign high performance corrosion inhibitors	272:321	novel benign high performance corrosion inhibitors based on biopolymer	272:341	Therefore, novel benign high performance corrosion inhibitors based on biopolymer were synthesized in-situ using different cellulosic materials and niacin.
32526303	4	26	theme	impedance	800:808	arg1	EIS					824:826	EIS	824:826	EIS	824:826	The anticorrosive performance of cellulose composites for copper in 3.5% NaCl solutions were evaluated using polarization and electrochemical impedance spectroscopy (EIS) techniques.
32526303	4	26	theme	impedance	800:808	arg1	spectroscopy					810:821	electrochemical impedance spectroscopy	784:821	electrochemical impedance spectroscopy (EIS)	784:827	The anticorrosive performance of cellulose composites for copper in 3.5% NaCl solutions were evaluated using polarization and electrochemical impedance spectroscopy (EIS) techniques.
32526303	0	27	theme	benign	16:21	arg1	inhibitors					33:42	Environmentally benign corrosion inhibitors	0:42	Environmentally benign corrosion inhibitors based on cellulose niacin	0:68	Environmentally benign corrosion inhibitors based on cellulose niacin nano-composite for corrosion of copper in sodium chloride solutions.
32526303	8	28	theme	corrosion	1437:1445	arg1	inhibition					1447:1456	the corrosion inhibition	1433:1456	the corrosion inhibition	1433:1456	The data extracted from EIS were fitted through an equivalent circuit to model the corrosion inhibition.
32526303	3	29	theme	nano-composite	451:464	arg1	Characterization					417:432	Characterization	417:432	Characterization of the cellulose nano-composite	417:464	Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX).
32526303	0	30	theme	Environmentally	0:14	arg1	inhibitors					33:42	Environmentally benign corrosion inhibitors	0:42	Environmentally benign corrosion inhibitors based on cellulose niacin	0:68	Environmentally benign corrosion inhibitors based on cellulose niacin nano-composite for corrosion of copper in sodium chloride solutions.
32526303	3	31	theme	scanning	588:595	arg1	SEM					618:620	SEM	618:620	SEM	618:620	Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX).
32526303	3	31	theme	scanning	588:595	arg1	microscopy					606:615	scanning electron microscopy	588:615	scanning electron microscopy (SEM)	588:621	Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX).
32526303	1	32	theme	effective	184:192	arg1	demand					155:160	a high demand	148:160	a high demand for high performance	148:181	There is a high demand for high performance, effective and eco-friendly corrosion inhibitors for industrial applications.
32526303	1	32	theme	effective	184:192	arg1	inhibitors					221:230	effective and eco-friendly corrosion inhibitors	184:230	effective and eco-friendly corrosion inhibitors for industrial applications	184:258	There is a high demand for high performance, effective and eco-friendly corrosion inhibitors for industrial applications.
32526303	5	33	theme	uninhibited	863:873	arg1	composites					889:898	uninhibited and inhibited composites	863:898	uninhibited and inhibited composites	863:898	Surface morphology of uninhibited and inhibited composites was studied using SEM and EDX.
32526303	4	34	theme	NaCl	731:734	arg1	solutions					736:744	3.5% NaCl solutions	726:744	3.5% NaCl solutions	726:744	The anticorrosive performance of cellulose composites for copper in 3.5% NaCl solutions were evaluated using polarization and electrochemical impedance spectroscopy (EIS) techniques.
32526303	7	35	from	Copper	1323:1328	arg1	solutions					1343:1351	3.5% NaCl solutions	1333:1351	3.5% NaCl solutions	1333:1351	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	8	36	theme	equivalent	1405:1414	arg1	circuit					1416:1422	an equivalent circuit	1402:1422	an equivalent circuit	1402:1422	The data extracted from EIS were fitted through an equivalent circuit to model the corrosion inhibition.
32526303	7	37	theme	%	1336:1336	arg1	solutions					1343:1351	3.5% NaCl solutions	1333:1351	3.5% NaCl solutions	1333:1351	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	0	38	theme	corrosion	23:31	arg1	inhibitors					33:42	Environmentally benign corrosion inhibitors	0:42	Environmentally benign corrosion inhibitors based on cellulose niacin	0:68	Environmentally benign corrosion inhibitors based on cellulose niacin nano-composite for corrosion of copper in sodium chloride solutions.
32526303	3	39	theme	dynamic	556:562	arg1	scattering					570:579	dynamic light scattering	556:579	dynamic light scattering (DLS)	556:585	Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX).
32526303	3	39	theme	dynamic	556:562	arg1	DLS					582:584	DLS	582:584	DLS	582:584	Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX).
32526303	4	40	theme	composites	701:710	arg1	performance					676:686	The anticorrosive performance	658:686	The anticorrosive performance of cellulose composites for copper in 3.5% NaCl solutions	658:744	The anticorrosive performance of cellulose composites for copper in 3.5% NaCl solutions were evaluated using polarization and electrochemical impedance spectroscopy (EIS) techniques.
32526303	1	41	theme	eco-friendly	198:209	arg1	demand					155:160	a high demand	148:160	a high demand for high performance	148:181	There is a high demand for high performance, effective and eco-friendly corrosion inhibitors for industrial applications.
32526303	1	41	theme	eco-friendly	198:209	arg1	inhibitors					221:230	effective and eco-friendly corrosion inhibitors	184:230	effective and eco-friendly corrosion inhibitors for industrial applications	184:258	There is a high demand for high performance, effective and eco-friendly corrosion inhibitors for industrial applications.
32526303	7	42	dep	%	1122:1122	arg1	outperforms					1124:1134	outperforms	1124:1134	outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions	1124:1351	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	0	43	from	corrosion	89:97	arg1	solutions					128:136	sodium chloride solutions	112:136	sodium chloride solutions	112:136	Environmentally benign corrosion inhibitors based on cellulose niacin nano-composite for corrosion of copper in sodium chloride solutions.
32526303	4	44	theme	anticorrosive	662:674	arg1	performance					676:686	The anticorrosive performance	658:686	The anticorrosive performance of cellulose composites for copper in 3.5% NaCl solutions	658:744	The anticorrosive performance of cellulose composites for copper in 3.5% NaCl solutions were evaluated using polarization and electrochemical impedance spectroscopy (EIS) techniques.
32526303	1	45	theme	corrosion	211:219	arg1	demand					155:160	a high demand	148:160	a high demand for high performance	148:181	There is a high demand for high performance, effective and eco-friendly corrosion inhibitors for industrial applications.
32526303	1	45	theme	corrosion	211:219	arg1	inhibitors					221:230	effective and eco-friendly corrosion inhibitors	184:230	effective and eco-friendly corrosion inhibitors for industrial applications	184:258	There is a high demand for high performance, effective and eco-friendly corrosion inhibitors for industrial applications.
32526303	5	46	theme	inhibited	879:887	arg1	composites					889:898	uninhibited and inhibited composites	863:898	uninhibited and inhibited composites	863:898	Surface morphology of uninhibited and inhibited composites was studied using SEM and EDX.
32526303	6	47	theme	based	998:1002	arg1	inhibitor					1035:1043	a mixed type inhibitor	1022:1043	a mixed type inhibitor	1022:1043	Potentiodynamic polarization measurements confirmed that cellulose based inhibitors act as a mixed type inhibitor.
32526303	6	47	theme	based	998:1002	arg1	inhibitors					1004:1013	cellulose based inhibitors	988:1013	cellulose based inhibitors	988:1013	Potentiodynamic polarization measurements confirmed that cellulose based inhibitors act as a mixed type inhibitor.
32526303	7	48	theme	carboxymethyl	1200:1212	arg1	composite					1231:1239	carboxymethyl cellulose-niacin composite	1200:1239	carboxymethyl cellulose-niacin composite (NCMC)	1200:1246	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	7	48	theme	carboxymethyl	1200:1212	arg1	NCMC					1242:1245	NCMC	1242:1245	NCMC	1242:1245	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	7	49	theme	cellulose-niacin	1214:1229	arg1	composite					1231:1239	carboxymethyl cellulose-niacin composite	1200:1239	carboxymethyl cellulose-niacin composite (NCMC)	1200:1246	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	7	49	theme	cellulose-niacin	1214:1229	arg1	NCMC					1242:1245	NCMC	1242:1245	NCMC	1242:1245	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	7	50	theme	3.5	1333:1335	arg1	%					1336:1336	%	1336:1336	%	1336:1336	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	3	51	theme	cellulose	441:449	arg1	nano-composite					451:464	the cellulose nano-composite	437:464	the cellulose nano-composite	437:464	Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX).
32526303	6	52	theme	cellulose	988:996	arg1	inhibitor					1035:1043	a mixed type inhibitor	1022:1043	a mixed type inhibitor	1022:1043	Potentiodynamic polarization measurements confirmed that cellulose based inhibitors act as a mixed type inhibitor.
32526303	6	52	theme	cellulose	988:996	arg1	inhibitors					1004:1013	cellulose based inhibitors	988:1013	cellulose based inhibitors	988:1013	Potentiodynamic polarization measurements confirmed that cellulose based inhibitors act as a mixed type inhibitor.
32526303	7	53	theme	composite	1098:1106	arg1	%					1122:1122	94.7%	1118:1122	94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions	1118:1351	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	7	53	theme	composite	1098:1106	arg1	efficiency					1061:1070	The inhibition efficiency	1046:1070	The inhibition efficiency of ethyl cellulose-niacin composite (NEC)	1046:1112	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	3	54	theme	electron	597:604	arg1	SEM					618:620	SEM	618:620	SEM	618:620	Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX).
32526303	3	54	theme	electron	597:604	arg1	microscopy					606:615	scanning electron microscopy	588:615	scanning electron microscopy (SEM)	588:621	Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX).
32526303	2	55	theme	different	374:382	arg1	materials					395:403	different cellulosic materials	374:403	different cellulosic materials	374:403	Therefore, novel benign high performance corrosion inhibitors based on biopolymer were synthesized in-situ using different cellulosic materials and niacin.
32526303	0	56	theme	cellulose	53:61	arg1	niacin					63:68	cellulose niacin	53:68	cellulose niacin	53:68	Environmentally benign corrosion inhibitors based on cellulose niacin nano-composite for corrosion of copper in sodium chloride solutions.
32526303	7	57	theme	green	1292:1296	arg1	inhibitors					1308:1317	green corrosion inhibitors	1292:1317	green corrosion inhibitors for Copper in 3.5% NaCl solutions	1292:1351	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	7	58	theme	ethyl	1075:1079	arg1	composite					1098:1106	ethyl cellulose-niacin composite	1075:1106	ethyl cellulose-niacin composite (NEC)	1075:1112	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	7	58	theme	ethyl	1075:1079	arg1	NEC					1109:1111	NEC	1109:1111	NEC	1109:1111	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	3	59	theme	energy	627:632	arg1	EDX					652:654	EDX	652:654	EDX	652:654	Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX).
32526303	3	59	theme	energy	627:632	arg1	X-ray					645:649	energy dispersive X-ray	627:649	energy dispersive X-ray (EDX)	627:655	Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX).
32526303	2	60	theme	benign	278:283	arg1	inhibitors					312:321	novel benign high performance corrosion inhibitors	272:321	novel benign high performance corrosion inhibitors based on biopolymer	272:341	Therefore, novel benign high performance corrosion inhibitors based on biopolymer were synthesized in-situ using different cellulosic materials and niacin.
32526303	7	61	theme	inhibition	1050:1059	arg1	%					1122:1122	94.7%	1118:1122	94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions	1118:1351	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	7	61	theme	inhibition	1050:1059	arg1	efficiency					1061:1070	The inhibition efficiency	1046:1070	The inhibition efficiency of ethyl cellulose-niacin composite (NEC)	1046:1112	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	4	62	theme	%	729:729	arg1	solutions					736:744	3.5% NaCl solutions	726:744	3.5% NaCl solutions	726:744	The anticorrosive performance of cellulose composites for copper in 3.5% NaCl solutions were evaluated using polarization and electrochemical impedance spectroscopy (EIS) techniques.
32526303	3	63	theme	light	564:568	arg1	scattering					570:579	dynamic light scattering	556:579	dynamic light scattering (DLS)	556:585	Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX).
32526303	3	63	theme	light	564:568	arg1	DLS					582:584	DLS	582:584	DLS	582:584	Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX).
32526303	2	64	theme	novel	272:276	arg1	inhibitors					312:321	novel benign high performance corrosion inhibitors	272:321	novel benign high performance corrosion inhibitors based on biopolymer	272:341	Therefore, novel benign high performance corrosion inhibitors based on biopolymer were synthesized in-situ using different cellulosic materials and niacin.
32526303	3	65	theme	thermal	520:526	arg1	TGA					550:552	TGA	550:552	TGA	550:552	Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX).
32526303	3	65	theme	thermal	520:526	arg1	analysis					540:547	thermal gravimetric analysis	520:547	thermal gravimetric analysis (TGA)	520:553	Characterization of the cellulose nano-composite was carried out by Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX).
32526303	1	66	theme	industrial	236:245	arg1	applications					247:258	industrial applications	236:258	industrial applications	236:258	There is a high demand for high performance, effective and eco-friendly corrosion inhibitors for industrial applications.
32526303	4	67	theme	cellulose	691:699	arg1	composites					701:710	cellulose composites	691:710	cellulose composites	691:710	The anticorrosive performance of cellulose composites for copper in 3.5% NaCl solutions were evaluated using polarization and electrochemical impedance spectroscopy (EIS) techniques.
32526303	4	68	from	copper	716:721	arg1	solutions					736:744	3.5% NaCl solutions	726:744	3.5% NaCl solutions	726:744	The anticorrosive performance of cellulose composites for copper in 3.5% NaCl solutions were evaluated using polarization and electrochemical impedance spectroscopy (EIS) techniques.
32526303	5	69	theme	Surface	841:847	arg1	morphology					849:858	Surface morphology	841:858	Surface morphology of uninhibited and inhibited composites	841:898	Surface morphology of uninhibited and inhibited composites was studied using SEM and EDX.
32526303	7	70	theme	corrosion	1298:1306	arg1	inhibitors					1308:1317	green corrosion inhibitors	1292:1317	green corrosion inhibitors for Copper in 3.5% NaCl solutions	1292:1351	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32526303	1	71	theme	high	150:153	arg1	demand					155:160	a high demand	148:160	a high demand for high performance	148:181	There is a high demand for high performance, effective and eco-friendly corrosion inhibitors for industrial applications.
32526303	1	71	theme	high	150:153	arg1	inhibitors					221:230	effective and eco-friendly corrosion inhibitors	184:230	effective and eco-friendly corrosion inhibitors for industrial applications	184:258	There is a high demand for high performance, effective and eco-friendly corrosion inhibitors for industrial applications.
32526303	7	72	theme	33.2	1259:1262	arg1	%					1272:1272	33.2 and 83.4%	1259:1272	%	1272:1272	The inhibition efficiency of ethyl cellulose-niacin composite (NEC) was 94.7% outperforms those of microcrystalline cellulose-niacin composite (NMCC) and carboxymethyl cellulose-niacin composite (NCMC) which were 33.2 and 83.4%, respectively, as green corrosion inhibitors for Copper in 3.5% NaCl solutions.
32777259	2	0	theme	various	515:521	arg1	blends					523:528	various blends	515:528	various blends	515:528	Whereas crystallo-co-spray drying of various blends has shown to improve the tabletting properties of poorly processable materials, the role of the solvent feed composition in altering the soluble fraction ratio of the excipient to the drug in a crystallo-co-spray dried agglomerate is not well understood.
32777259	2	1	theme	solvent	626:632	arg1	composition					639:649	the solvent feed composition	622:649	the solvent feed composition	622:649	Whereas crystallo-co-spray drying of various blends has shown to improve the tabletting properties of poorly processable materials, the role of the solvent feed composition in altering the soluble fraction ratio of the excipient to the drug in a crystallo-co-spray dried agglomerate is not well understood.
32777259	2	2	theme	crystallo-co-spray	724:741	arg1	agglomerate					749:759	a crystallo-co-spray dried agglomerate	722:759	a crystallo-co-spray dried agglomerate	722:759	Whereas crystallo-co-spray drying of various blends has shown to improve the tabletting properties of poorly processable materials, the role of the solvent feed composition in altering the soluble fraction ratio of the excipient to the drug in a crystallo-co-spray dried agglomerate is not well understood.
32777259	4	3	theme	final	1275:1279	arg1	blend					1300:1304	the final spray dried powder blend	1271:1304	the final spray dried powder blend	1271:1304	It was found that an increase in excipient soluble fraction in the inlet feed resulted in a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug.
32777259	3	4	from	fraction	1103:1110	arg1	feed					1136:1139	feed	1136:1139	feed	1136:1139	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	0	5	theme	compaction	51:60	arg1	properties					62:71	the compaction properties	47:71	the compaction properties of materials	47:84	The impact of the degree of intimate mixing on the compaction properties of materials produced by crystallo-co-spray drying.
32777259	0	6	from	impact	4:9	arg1	properties					62:71	the compaction properties	47:71	the compaction properties of materials	47:84	The impact of the degree of intimate mixing on the compaction properties of materials produced by crystallo-co-spray drying.
32777259	3	7	theme	co-spray	1012:1019	arg1	drying					1021:1026	co-spray drying	1012:1026	co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed	1012:1139	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	3	8	theme	drug	863:866	arg1	fraction					849:856	the soluble fraction	837:856	the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate)	837:921	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	4	9	theme	mixing	1261:1266	arg1	degree					1242:1247	a greater degree	1232:1247	a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug	1232:1397	It was found that an increase in excipient soluble fraction in the inlet feed resulted in a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug.
32777259	0	10	theme	materials	76:84	arg1	properties					62:71	the compaction properties	47:71	the compaction properties of materials	47:84	The impact of the degree of intimate mixing on the compaction properties of materials produced by crystallo-co-spray drying.
32777259	3	11	theme	feed	1048:1051	arg1	compositions					1061:1072	various inlet feed solvent compositions	1034:1072	various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed	1034:1139	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	3	12	theme	solvent	1053:1059	arg1	compositions					1061:1072	various inlet feed solvent compositions	1034:1072	various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed	1034:1139	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	2	13	theme	fraction	675:682	arg1	ratio					684:688	the soluble fraction ratio	663:688	the soluble fraction ratio of the excipient to the drug in a crystallo-co-spray dried agglomerate	663:759	Whereas crystallo-co-spray drying of various blends has shown to improve the tabletting properties of poorly processable materials, the role of the solvent feed composition in altering the soluble fraction ratio of the excipient to the drug in a crystallo-co-spray dried agglomerate is not well understood.
32777259	3	14	theme	crystallo-co-spray	961:978	arg1	agglomerates					986:997	their crystallo-co-spray dried agglomerates	955:997	their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed	955:1139	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	4	15	theme	processable	1382:1392	arg1	drug					1394:1397	the poorly processable drug	1371:1397	the poorly processable drug	1371:1397	It was found that an increase in excipient soluble fraction in the inlet feed resulted in a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug.
32777259	4	16	theme	spray	1281:1285	arg1	blend					1300:1304	the final spray dried powder blend	1271:1304	the final spray dried powder blend	1271:1304	It was found that an increase in excipient soluble fraction in the inlet feed resulted in a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug.
32777259	4	17	from	blend	1300:1304	arg1	degree					1242:1247	a greater degree	1232:1247	a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug	1232:1397	It was found that an increase in excipient soluble fraction in the inlet feed resulted in a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug.
32777259	1	18	theme	deformation	454:464	arg1	mechanisms					466:475	deformation mechanisms	454:475	deformation mechanisms	454:475	Direct compression remains one of the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms.
32777259	3	19	theme	excipient	889:897	arg1	fraction					849:856	the soluble fraction	837:856	the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate)	837:921	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	3	20	from	excipient	1119:1127	arg1	feed					1136:1139	feed	1136:1139	feed	1136:1139	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	1	21	theme	formulations	349:360	arg1	majority					337:344	the majority	333:344	the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms	333:475	Direct compression remains one of the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms.
32777259	2	22	theme	soluble	667:673	arg1	fraction					675:682	the soluble fraction	663:682	the soluble fraction ratio of the excipient to the drug in a crystallo-co-spray dried agglomerate	663:759	Whereas crystallo-co-spray drying of various blends has shown to improve the tabletting properties of poorly processable materials, the role of the solvent feed composition in altering the soluble fraction ratio of the excipient to the drug in a crystallo-co-spray dried agglomerate is not well understood.
32777259	1	23	theme	due	362:364	arg1	formulations					349:360	formulations	349:360	formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms	349:475	Direct compression remains one of the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms.
32777259	3	24	theme	soluble	1095:1101	arg1	fraction					1103:1110	the soluble fraction	1091:1110	the soluble fraction of the excipient in the feed	1091:1139	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	3	25	theme	inlet	1042:1046	arg1	compositions					1061:1072	various inlet feed solvent compositions	1034:1072	various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed	1034:1139	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	4	26	theme	drug	1394:1397	arg1	properties					1357:1366	tabletting properties	1346:1366	tabletting properties of the poorly processable drug	1346:1397	It was found that an increase in excipient soluble fraction in the inlet feed resulted in a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug.
32777259	3	27	theme	excipient	1119:1127	arg1	fraction					1103:1110	the soluble fraction	1091:1110	the soluble fraction of the excipient in the feed	1091:1139	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	4	28	theme	dried	1287:1291	arg1	blend					1300:1304	the final spray dried powder blend	1271:1304	the final spray dried powder blend	1271:1304	It was found that an increase in excipient soluble fraction in the inlet feed resulted in a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug.
32777259	2	29	theme	composition	639:649	arg1	role					614:617	the role	610:617	the role of the solvent feed composition in altering the soluble fraction ratio of the excipient to the drug in a crystallo-co-spray dried agglomerate	610:759	Whereas crystallo-co-spray drying of various blends has shown to improve the tabletting properties of poorly processable materials, the role of the solvent feed composition in altering the soluble fraction ratio of the excipient to the drug in a crystallo-co-spray dried agglomerate is not well understood.
32777259	2	30	theme	excipient	697:705	arg1	ratio					684:688	the soluble fraction ratio	663:688	the soluble fraction ratio of the excipient to the drug in a crystallo-co-spray dried agglomerate	663:759	Whereas crystallo-co-spray drying of various blends has shown to improve the tabletting properties of poorly processable materials, the role of the solvent feed composition in altering the soluble fraction ratio of the excipient to the drug in a crystallo-co-spray dried agglomerate is not well understood.
32777259	4	31	theme	soluble	1185:1191	arg1	fraction					1193:1200	excipient soluble fraction	1175:1200	excipient soluble fraction in the inlet feed	1175:1218	It was found that an increase in excipient soluble fraction in the inlet feed resulted in a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug.
32777259	1	32	theme	favourable	168:177	arg1	methods					179:185	the most favourable methods	159:185	the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms	159:475	Direct compression remains one of the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms.
32777259	1	33	theme	cost	262:265	arg1	effectiveness					267:279	cost effectiveness	262:279	cost effectiveness	262:279	Direct compression remains one of the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms.
32777259	2	34	theme	feed	634:637	arg1	composition					639:649	the solvent feed composition	622:649	the solvent feed composition	622:649	Whereas crystallo-co-spray drying of various blends has shown to improve the tabletting properties of poorly processable materials, the role of the solvent feed composition in altering the soluble fraction ratio of the excipient to the drug in a crystallo-co-spray dried agglomerate is not well understood.
32777259	1	35	theme	methods	179:185	arg1	methods					179:185	the most favourable methods	159:185	the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms	159:475	Direct compression remains one of the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms.
32777259	1	35	theme	methods	179:185	arg1	one					152:154	one	152:154	one	152:154	Direct compression remains one of the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms.
32777259	3	36	theme	various	1034:1040	arg1	compositions					1061:1072	various inlet feed solvent compositions	1034:1072	various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed	1034:1139	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	4	37	from	fraction	1193:1200	arg1	feed					1215:1218	the inlet feed	1205:1218	the inlet feed	1205:1218	It was found that an increase in excipient soluble fraction in the inlet feed resulted in a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug.
32777259	1	38	theme	available	187:195	arg1	methods					179:185	the most favourable methods	159:185	the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms	159:475	Direct compression remains one of the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms.
32777259	4	39	from	improvement	1331:1341	arg1	properties					1357:1366	tabletting properties	1346:1366	tabletting properties of the poorly processable drug	1346:1397	It was found that an increase in excipient soluble fraction in the inlet feed resulted in a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug.
32777259	0	40	theme	crystallo-co-spray	98:115	arg1	drying					117:122	crystallo-co-spray drying	98:122	crystallo-co-spray drying	98:122	The impact of the degree of intimate mixing on the compaction properties of materials produced by crystallo-co-spray drying.
32777259	3	41	from	role	829:832	arg1	properties					941:950	the tabletting properties	926:950	the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed	926:1139	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	3	42	theme	α-lactose	900:908	arg1	excipient					889:897	an excipient	886:897	an excipient (α-lactose monohydrate)	886:921	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	3	42	theme	α-lactose	900:908	arg1	monohydrate					910:920	α-lactose monohydrate	900:920	α-lactose monohydrate	900:920	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	4	43	theme	tabletting	1346:1355	arg1	properties					1357:1366	tabletting properties	1346:1366	tabletting properties of the poorly processable drug	1346:1397	It was found that an increase in excipient soluble fraction in the inlet feed resulted in a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug.
32777259	4	44	from	mixing	1261:1266	arg1	blend					1300:1304	the final spray dried powder blend	1271:1304	the final spray dried powder blend	1271:1304	It was found that an increase in excipient soluble fraction in the inlet feed resulted in a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug.
32777259	4	45	theme	intimate	1252:1259	arg1	mixing					1261:1266	intimate mixing	1252:1266	intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug	1252:1397	It was found that an increase in excipient soluble fraction in the inlet feed resulted in a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug.
32777259	0	46	theme	degree	18:23	arg1	impact					4:9	The impact	0:9	The impact of the degree of intimate mixing on the compaction properties of materials	0:84	The impact of the degree of intimate mixing on the compaction properties of materials produced by crystallo-co-spray drying.
32777259	2	47	theme	crystallo-co-spray	486:503	arg1	drying					505:510	crystallo-co-spray drying	486:510	crystallo-co-spray drying of various blends	486:528	Whereas crystallo-co-spray drying of various blends has shown to improve the tabletting properties of poorly processable materials, the role of the solvent feed composition in altering the soluble fraction ratio of the excipient to the drug in a crystallo-co-spray dried agglomerate is not well understood.
32777259	1	48	theme	poor	390:393	arg1	compressibility					434:448	inadequate compressibility	423:448	inadequate compressibility	423:448	Direct compression remains one of the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms.
32777259	1	48	theme	poor	390:393	arg1	mechanisms					466:475	deformation mechanisms	454:475	deformation mechanisms	454:475	Direct compression remains one of the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms.
32777259	1	48	theme	poor	390:393	arg1	properties					404:413	poor physical properties	390:413	poor physical properties such as inadequate compressibility and deformation mechanisms	390:475	Direct compression remains one of the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms.
32777259	3	49	from	feed	1136:1139	arg1	fraction					1103:1110	the soluble fraction	1091:1110	the soluble fraction of the excipient in the feed	1091:1139	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	2	50	theme	tabletting	555:564	arg1	properties					566:575	the tabletting properties	551:575	the tabletting properties of poorly processable materials	551:607	Whereas crystallo-co-spray drying of various blends has shown to improve the tabletting properties of poorly processable materials, the role of the solvent feed composition in altering the soluble fraction ratio of the excipient to the drug in a crystallo-co-spray dried agglomerate is not well understood.
32777259	1	51	theme	tablet	208:213	arg1	compacts					215:222	tablet compacts	208:222	tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms	208:475	Direct compression remains one of the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms.
32777259	0	52	theme	mixing	37:42	arg1	degree					18:23	the degree	14:23	the degree of intimate mixing	14:42	The impact of the degree of intimate mixing on the compaction properties of materials produced by crystallo-co-spray drying.
32777259	1	53	theme	physical	395:402	arg1	compressibility					434:448	inadequate compressibility	423:448	inadequate compressibility	423:448	Direct compression remains one of the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms.
32777259	1	53	theme	physical	395:402	arg1	mechanisms					466:475	deformation mechanisms	454:475	deformation mechanisms	454:475	Direct compression remains one of the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms.
32777259	1	53	theme	physical	395:402	arg1	properties					404:413	poor physical properties	390:413	poor physical properties such as inadequate compressibility and deformation mechanisms	390:475	Direct compression remains one of the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms.
32777259	4	54	from	increase	1163:1170	arg1	fraction					1193:1200	excipient soluble fraction	1175:1200	excipient soluble fraction in the inlet feed	1175:1218	It was found that an increase in excipient soluble fraction in the inlet feed resulted in a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug.
32777259	4	55	theme	powder	1293:1298	arg1	blend					1300:1304	the final spray dried powder blend	1271:1304	the final spray dried powder blend	1271:1304	It was found that an increase in excipient soluble fraction in the inlet feed resulted in a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug.
32777259	0	56	theme	intimate	28:35	arg1	mixing					37:42	intimate mixing	28:42	intimate mixing	28:42	The impact of the degree of intimate mixing on the compaction properties of materials produced by crystallo-co-spray drying.
32777259	4	57	from	degree	1242:1247	arg1	blend					1300:1304	the final spray dried powder blend	1271:1304	the final spray dried powder blend	1271:1304	It was found that an increase in excipient soluble fraction in the inlet feed resulted in a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug.
32777259	4	58	theme	greater	1234:1240	arg1	degree					1242:1247	a greater degree	1232:1247	a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug	1232:1397	It was found that an increase in excipient soluble fraction in the inlet feed resulted in a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug.
32777259	4	59	theme	excipient	1175:1183	arg1	fraction					1193:1200	excipient soluble fraction	1175:1200	excipient soluble fraction in the inlet feed	1175:1218	It was found that an increase in excipient soluble fraction in the inlet feed resulted in a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug.
32777259	2	60	from	drug	714:717	arg1	agglomerate					749:759	a crystallo-co-spray dried agglomerate	722:759	a crystallo-co-spray dried agglomerate	722:759	Whereas crystallo-co-spray drying of various blends has shown to improve the tabletting properties of poorly processable materials, the role of the solvent feed composition in altering the soluble fraction ratio of the excipient to the drug in a crystallo-co-spray dried agglomerate is not well understood.
32777259	2	61	theme	materials	599:607	arg1	properties					566:575	the tabletting properties	551:575	the tabletting properties of poorly processable materials	551:607	Whereas crystallo-co-spray drying of various blends has shown to improve the tabletting properties of poorly processable materials, the role of the solvent feed composition in altering the soluble fraction ratio of the excipient to the drug in a crystallo-co-spray dried agglomerate is not well understood.
32777259	3	62	theme	tabletting	930:939	arg1	properties					941:950	the tabletting properties	926:950	the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed	926:1139	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	2	63	theme	processable	587:597	arg1	materials					599:607	poorly processable materials	580:607	poorly processable materials	580:607	Whereas crystallo-co-spray drying of various blends has shown to improve the tabletting properties of poorly processable materials, the role of the solvent feed composition in altering the soluble fraction ratio of the excipient to the drug in a crystallo-co-spray dried agglomerate is not well understood.
32777259	1	64	dep	simplicity	235:244	arg1	remains					310:316	remains	310:316	remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms	310:475	Direct compression remains one of the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms.
32777259	3	65	theme	dried	980:984	arg1	agglomerates					986:997	their crystallo-co-spray dried agglomerates	955:997	their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed	955:1139	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	1	66	theme	Direct	125:130	arg1	compression					132:142	Direct compression	125:142	Direct compression	125:142	Direct compression remains one of the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms.
32777259	3	67	theme	soluble	841:847	arg1	fraction					849:856	the soluble fraction	837:856	the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate)	837:921	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	1	68	theme	due	224:226	arg1	compacts					215:222	tablet compacts	208:222	tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms	208:475	Direct compression remains one of the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms.
32777259	1	69	theme	inadequate	423:432	arg1	compressibility					434:448	inadequate compressibility	423:448	inadequate compressibility	423:448	Direct compression remains one of the most favourable methods available to produce tablet compacts due to its simplicity, efficiency and cost effectiveness however, the technique still remains unsuitable for the majority of formulations due to materials exhibiting poor physical properties such as inadequate compressibility and deformation mechanisms.
32777259	3	70	theme	agglomerates	986:997	arg1	properties					941:950	the tabletting properties	926:950	the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed	926:1139	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	3	71	theme	fraction	849:856	arg1	role					829:832	the role	825:832	the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed	825:1139	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	3	72	theme	work	801:804	arg1	aim					789:791	The aim	785:791	The aim of this work	785:804	The aim of this work was to investigate the role of the soluble fraction of a drug (paracetamol) and an excipient (α-lactose monohydrate) on the tabletting properties of their crystallo-co-spray dried agglomerates produced via co-spray drying using various inlet feed solvent compositions in order to vary the soluble fraction of the excipient in the feed.
32777259	2	73	theme	blends	523:528	arg1	drying					505:510	crystallo-co-spray drying	486:510	crystallo-co-spray drying of various blends	486:528	Whereas crystallo-co-spray drying of various blends has shown to improve the tabletting properties of poorly processable materials, the role of the solvent feed composition in altering the soluble fraction ratio of the excipient to the drug in a crystallo-co-spray dried agglomerate is not well understood.
32777259	2	74	theme	dried	743:747	arg1	agglomerate					749:759	a crystallo-co-spray dried agglomerate	722:759	a crystallo-co-spray dried agglomerate	722:759	Whereas crystallo-co-spray drying of various blends has shown to improve the tabletting properties of poorly processable materials, the role of the solvent feed composition in altering the soluble fraction ratio of the excipient to the drug in a crystallo-co-spray dried agglomerate is not well understood.
32777259	4	75	theme	inlet	1209:1213	arg1	feed					1215:1218	the inlet feed	1205:1218	the inlet feed	1205:1218	It was found that an increase in excipient soluble fraction in the inlet feed resulted in a greater degree of intimate mixing in the final spray dried powder blend, which in turn led to an improvement in tabletting properties of the poorly processable drug.
32709970	0	0	theme	dikaryon	101:108	arg1	cultures					120:127	monokaryon and dikaryon submerged cultures	86:127	monokaryon and dikaryon submerged cultures of the white-rot basidiomycete	86:158	Glucose counteracts wood-dependent induction of lignocellulolytic enzyme secretion in monokaryon and dikaryon submerged cultures of the white-rot basidiomycete Pleurotus ostreatus.
32709970	2	1	theme	wall	529:532	arg1	components					500:509	all the components	492:509	all the components of the plant cell wall	492:532	The study revealed that this white-rot basidiomycete attacks all the components of the plant cell wall.
32709970	1	2	theme	secretome	185:193	arg1	complexity					195:204	The secretome complexity and lignocellulose degrading capacity	181:242	complexity	195:204	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	1	3	theme	mated	300:304	arg1	dkN001					315:320	mated dikaryon dkN001	300:320	mated dikaryon dkN001	300:320	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	2	4	theme	cell	524:527	arg1	wall					529:532	the plant cell wall	514:532	the plant cell wall	514:532	The study revealed that this white-rot basidiomycete attacks all the components of the plant cell wall.
32709970	4	5	theme	secretome	726:734	arg1	complexity					736:745	the secretome complexity	722:745	the secretome complexity	722:745	The presence of wood increased the secretome complexity, whereas glucose diminished the secretion of enzymes involved in cellulose, hemicellulose and pectin degradation.
32709970	1	6	theme	dikaryon	306:313	arg1	dkN001					315:320	mated dikaryon dkN001	300:320	mated dikaryon dkN001	300:320	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	5	7	theme	cellulolytic	1020:1031	arg1	enzymes					1033:1039	cellulolytic enzymes	1020:1039	cellulolytic enzymes	1020:1039	In contrast, the presence of glucose did not influence the secretion of redox enzymes or proteases, which shows the specificity of glucose on the secretion of cellulolytic enzymes.
32709970	3	8	theme	hydrolases	580:589	arg1	hydrolases					580:589	glycoside hydrolases	570:589	glycoside hydrolases	570:589	P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source.
32709970	3	8	theme	hydrolases	580:589	arg1	esterases					605:613	carbohydrate esterases	592:613	carbohydrate esterases	592:613	P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source.
32709970	3	8	theme	hydrolases	580:589	arg1	lyases					635:640	polysaccharide lyases	620:640	polysaccharide lyases	620:640	P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source.
32709970	3	8	theme	hydrolases	580:589	arg1	variety					559:565	a variety	557:565	a variety	557:565	P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source.
32709970	0	9	theme	submerged	110:118	arg1	cultures					120:127	monokaryon and dikaryon submerged cultures	86:127	monokaryon and dikaryon submerged cultures of the white-rot basidiomycete	86:158	Glucose counteracts wood-dependent induction of lignocellulolytic enzyme secretion in monokaryon and dikaryon submerged cultures of the white-rot basidiomycete Pleurotus ostreatus.
32709970	5	10	theme	redox	933:937	arg1	enzymes					939:945	redox enzymes	933:945	redox enzymes	933:945	In contrast, the presence of glucose did not influence the secretion of redox enzymes or proteases, which shows the specificity of glucose on the secretion of cellulolytic enzymes.
32709970	6	11	theme	monokaryons	1078:1088	arg1	secretomes					1064:1073	the secretomes	1060:1073	the secretomes of monokaryons and dikaryons	1060:1102	The comparison of the secretomes of monokaryons and dikaryons reveals that secretome complexity is unrelated to the nuclear composition of the strain.
32709970	4	12	theme	wood	707:710	arg1	presence					695:702	The presence	691:702	The presence of wood	691:710	The presence of wood increased the secretome complexity, whereas glucose diminished the secretion of enzymes involved in cellulose, hemicellulose and pectin degradation.
32709970	6	13	theme	secretome	1117:1125	arg1	complexity					1127:1136	secretome complexity	1117:1136	secretome complexity	1117:1136	The comparison of the secretomes of monokaryons and dikaryons reveals that secretome complexity is unrelated to the nuclear composition of the strain.
32709970	5	14	theme	enzymes	939:945	arg1	secretion					920:928	the secretion	916:928	the secretion	916:928	In contrast, the presence of glucose did not influence the secretion of redox enzymes or proteases, which shows the specificity of glucose on the secretion of cellulolytic enzymes.
32709970	2	15	theme	plant	518:522	arg1	wall					529:532	the plant cell wall	514:532	the plant cell wall	514:532	The study revealed that this white-rot basidiomycete attacks all the components of the plant cell wall.
32709970	6	16	theme	strain	1185:1190	arg1	composition					1166:1176	the nuclear composition	1154:1176	the nuclear composition of the strain	1154:1190	The comparison of the secretomes of monokaryons and dikaryons reveals that secretome complexity is unrelated to the nuclear composition of the strain.
32709970	3	17	theme	glycoside	570:578	arg1	hydrolases					580:589	glycoside hydrolases	570:589	glycoside hydrolases	570:589	P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source.
32709970	1	18	theme	lignocellulose	210:223	arg1	capacity					235:242	The secretome complexity and lignocellulose degrading capacity	181:242	capacity	235:242	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	1	19	theme	carbon	415:420	arg1	sources					422:428	carbon sources	415:428	carbon sources	415:428	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	1	19	theme	carbon	415:420	arg1	wood					394:397	wood	394:397	wood	394:397	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	1	19	theme	carbon	415:420	arg1	wood					375:378	wood	375:378	wood	375:378	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	1	19	theme	carbon	415:420	arg1	glucose					381:387	glucose	381:387	glucose	381:387	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	4	20	theme	enzymes	792:798	arg1	secretion					779:787	the secretion	775:787	the secretion of enzymes involved in cellulose, hemicellulose and pectin degradation	775:858	The presence of wood increased the secretome complexity, whereas glucose diminished the secretion of enzymes involved in cellulose, hemicellulose and pectin degradation.
32709970	1	21	theme	degrading	225:233	arg1	capacity					235:242	The secretome complexity and lignocellulose degrading capacity	181:242	capacity	235:242	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	6	22	theme	dikaryons	1094:1102	arg1	secretomes					1064:1073	the secretomes	1060:1073	the secretomes of monokaryons and dikaryons	1060:1102	The comparison of the secretomes of monokaryons and dikaryons reveals that secretome complexity is unrelated to the nuclear composition of the strain.
32709970	0	23	theme	basidiomycete	146:158	arg1	cultures					120:127	monokaryon and dikaryon submerged cultures	86:127	monokaryon and dikaryon submerged cultures of the white-rot basidiomycete	86:158	Glucose counteracts wood-dependent induction of lignocellulolytic enzyme secretion in monokaryon and dikaryon submerged cultures of the white-rot basidiomycete Pleurotus ostreatus.
32709970	1	24	contain	containing	364:373	arg2	glucose					381:387	glucose	381:387	glucose	381:387	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	1	24	contain	containing	364:373	arg2	wood					375:378	wood	375:378	wood	375:378	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	1	24	contain	containing	364:373	arg2	wood					394:397	wood	394:397	wood	394:397	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	1	24	contain	containing	364:373	arg1	cultures					355:362	submerged liquid cultures	338:362	submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources	338:428	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	1	24	contain	containing	364:373	arg2	sources					422:428	carbon sources	415:428	carbon sources	415:428	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	2	25	theme	white-rot	460:468	arg1	basidiomycete					470:482	this white-rot basidiomycete	455:482	this white-rot basidiomycete	455:482	The study revealed that this white-rot basidiomycete attacks all the components of the plant cell wall.
32709970	0	26	theme	wood-dependent	20:33	arg1	induction					35:43	wood-dependent induction	20:43	wood-dependent induction of lignocellulolytic enzyme secretion in monokaryon and dikaryon submerged cultures of the white-rot basidiomycete	20:158	Glucose counteracts wood-dependent induction of lignocellulolytic enzyme secretion in monokaryon and dikaryon submerged cultures of the white-rot basidiomycete Pleurotus ostreatus.
32709970	1	27	theme	submerged	338:346	arg1	cultures					355:362	submerged liquid cultures	338:362	submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources	338:428	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	0	28	theme	white-rot	136:144	arg1	basidiomycete					146:158	the white-rot basidiomycete	132:158	the white-rot basidiomycete	132:158	Glucose counteracts wood-dependent induction of lignocellulolytic enzyme secretion in monokaryon and dikaryon submerged cultures of the white-rot basidiomycete Pleurotus ostreatus.
32709970	3	29	theme	esterases	605:613	arg1	hydrolases					580:589	glycoside hydrolases	570:589	glycoside hydrolases	570:589	P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source.
32709970	3	29	theme	esterases	605:613	arg1	esterases					605:613	carbohydrate esterases	592:613	carbohydrate esterases	592:613	P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source.
32709970	3	29	theme	esterases	605:613	arg1	lyases					635:640	polysaccharide lyases	620:640	polysaccharide lyases	620:640	P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source.
32709970	3	29	theme	esterases	605:613	arg1	variety					559:565	a variety	557:565	a variety	557:565	P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source.
32709970	1	30	theme	Pleurotus	247:255	arg1	mkPC9					279:283	Pleurotus ostreatus monokaryons mkPC9	247:283	Pleurotus ostreatus monokaryons mkPC9	247:283	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	0	31	theme	lignocellulolytic	48:64	arg1	secretion					73:81	lignocellulolytic enzyme secretion	48:81	lignocellulolytic enzyme secretion	48:81	Glucose counteracts wood-dependent induction of lignocellulolytic enzyme secretion in monokaryon and dikaryon submerged cultures of the white-rot basidiomycete Pleurotus ostreatus.
32709970	1	32	theme	liquid	348:353	arg1	cultures					355:362	submerged liquid cultures	338:362	submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources	338:428	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	3	33	theme	only	671:674	arg1	source					683:688	the only carbon source	667:688	the only carbon source	667:688	P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source.
32709970	3	33	theme	only	671:674	arg1	wood					659:662	wood	659:662	wood	659:662	P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source.
32709970	1	34	theme	monokaryons	267:277	arg1	mkPC9					279:283	Pleurotus ostreatus monokaryons mkPC9	247:283	Pleurotus ostreatus monokaryons mkPC9	247:283	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	0	35	theme	secretion	73:81	arg1	induction					35:43	wood-dependent induction	20:43	wood-dependent induction of lignocellulolytic enzyme secretion in monokaryon and dikaryon submerged cultures of the white-rot basidiomycete	20:158	Glucose counteracts wood-dependent induction of lignocellulolytic enzyme secretion in monokaryon and dikaryon submerged cultures of the white-rot basidiomycete Pleurotus ostreatus.
32709970	6	36	theme	secretomes	1064:1073	arg1	comparison					1046:1055	The comparison	1042:1055	The comparison of the secretomes of monokaryons and dikaryons	1042:1102	The comparison of the secretomes of monokaryons and dikaryons reveals that secretome complexity is unrelated to the nuclear composition of the strain.
32709970	3	37	theme	carbon	676:681	arg1	source					683:688	the only carbon source	667:688	the only carbon source	667:688	P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source.
32709970	3	37	theme	carbon	676:681	arg1	wood					659:662	wood	659:662	wood	659:662	P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source.
32709970	3	38	theme	polysaccharide	620:633	arg1	lyases					635:640	polysaccharide lyases	620:640	polysaccharide lyases	620:640	P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source.
32709970	0	39	theme	enzyme	66:71	arg1	secretion					73:81	lignocellulolytic enzyme secretion	48:81	lignocellulolytic enzyme secretion	48:81	Glucose counteracts wood-dependent induction of lignocellulolytic enzyme secretion in monokaryon and dikaryon submerged cultures of the white-rot basidiomycete Pleurotus ostreatus.
32709970	3	40	theme	lyases	635:640	arg1	hydrolases					580:589	glycoside hydrolases	570:589	glycoside hydrolases	570:589	P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source.
32709970	3	40	theme	lyases	635:640	arg1	esterases					605:613	carbohydrate esterases	592:613	carbohydrate esterases	592:613	P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source.
32709970	3	40	theme	lyases	635:640	arg1	lyases					635:640	polysaccharide lyases	620:640	polysaccharide lyases	620:640	P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source.
32709970	3	40	theme	lyases	635:640	arg1	variety					559:565	a variety	557:565	a variety	557:565	P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source.
32709970	5	41	theme	proteases	950:958	arg1	secretion					920:928	the secretion	916:928	the secretion	916:928	In contrast, the presence of glucose did not influence the secretion of redox enzymes or proteases, which shows the specificity of glucose on the secretion of cellulolytic enzymes.
32709970	4	42	theme	pectin	841:846	arg1	degradation					848:858	pectin degradation	841:858	pectin degradation	841:858	The presence of wood increased the secretome complexity, whereas glucose diminished the secretion of enzymes involved in cellulose, hemicellulose and pectin degradation.
32709970	6	43	theme	nuclear	1158:1164	arg1	composition					1166:1176	the nuclear composition	1154:1176	the nuclear composition of the strain	1154:1190	The comparison of the secretomes of monokaryons and dikaryons reveals that secretome complexity is unrelated to the nuclear composition of the strain.
32709970	3	44	theme	carbohydrate	592:603	arg1	esterases					605:613	carbohydrate esterases	592:613	carbohydrate esterases	592:613	P. ostreatus secretes a variety of glycoside hydrolases, carbohydrate esterases, and polysaccharide lyases, especially when wood is the only carbon source.
32709970	1	45	theme	mkPC9	279:283	arg1	capacity					235:242	The secretome complexity and lignocellulose degrading capacity	181:242	capacity	235:242	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	1	45	theme	mkPC9	279:283	arg1	dkN001					315:320	mated dikaryon dkN001	300:320	mated dikaryon dkN001	300:320	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	1	45	theme	mkPC9	279:283	arg1	complexity					195:204	The secretome complexity and lignocellulose degrading capacity	181:242	complexity	195:204	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	4	46	dep	increased	712:720	arg1	whereas					748:754	whereas	748:754	whereas	748:754	The presence of wood increased the secretome complexity, whereas glucose diminished the secretion of enzymes involved in cellulose, hemicellulose and pectin degradation.
32709970	5	47	theme	glucose	992:998	arg1	specificity					977:987	the specificity	973:987	the specificity of glucose	973:998	In contrast, the presence of glucose did not influence the secretion of redox enzymes or proteases, which shows the specificity of glucose on the secretion of cellulolytic enzymes.
32709970	0	48	theme	monokaryon	86:95	arg1	cultures					120:127	monokaryon and dikaryon submerged cultures	86:127	monokaryon and dikaryon submerged cultures of the white-rot basidiomycete	86:158	Glucose counteracts wood-dependent induction of lignocellulolytic enzyme secretion in monokaryon and dikaryon submerged cultures of the white-rot basidiomycete Pleurotus ostreatus.
32709970	5	49	theme	glucose	890:896	arg1	presence					878:885	the presence	874:885	the presence of glucose	874:896	In contrast, the presence of glucose did not influence the secretion of redox enzymes or proteases, which shows the specificity of glucose on the secretion of cellulolytic enzymes.
32709970	5	50	theme	enzymes	1033:1039	arg1	secretion					1007:1015	the secretion	1003:1015	the secretion of cellulolytic enzymes	1003:1039	In contrast, the presence of glucose did not influence the secretion of redox enzymes or proteases, which shows the specificity of glucose on the secretion of cellulolytic enzymes.
32709970	1	51	theme	mkPC15	289:294	arg1	capacity					235:242	The secretome complexity and lignocellulose degrading capacity	181:242	capacity	235:242	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	1	51	theme	mkPC15	289:294	arg1	dkN001					315:320	mated dikaryon dkN001	300:320	mated dikaryon dkN001	300:320	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	1	51	theme	mkPC15	289:294	arg1	complexity					195:204	The secretome complexity and lignocellulose degrading capacity	181:242	complexity	195:204	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
32709970	0	52	from	induction	35:43	arg1	cultures					120:127	monokaryon and dikaryon submerged cultures	86:127	monokaryon and dikaryon submerged cultures of the white-rot basidiomycete	86:158	Glucose counteracts wood-dependent induction of lignocellulolytic enzyme secretion in monokaryon and dikaryon submerged cultures of the white-rot basidiomycete Pleurotus ostreatus.
32709970	1	53	dep	Pleurotus	247:255	arg1	ostreatus					257:265	ostreatus	257:265	ostreatus	257:265	The secretome complexity and lignocellulose degrading capacity of Pleurotus ostreatus monokaryons mkPC9 and mkPC15 and mated dikaryon dkN001 were studied in submerged liquid cultures containing wood, glucose, and wood plus glucose as carbon sources.
34060150	5	0	theme	mechanical	746:755	arg1	coverage					813:820	high coverage	808:820	high coverage	808:820	The ability to engineer key mechanical characteristics such as dynamic yield stresses and high coverage is shown, through systematic understanding of the composite mixture containing both gellan and λ-carrageenan.
34060150	5	0	theme	mechanical	746:755	arg1	stresses					795:802	dynamic yield stresses	781:802	dynamic yield stresses	781:802	The ability to engineer key mechanical characteristics such as dynamic yield stresses and high coverage is shown, through systematic understanding of the composite mixture containing both gellan and λ-carrageenan.
34060150	5	0	theme	mechanical	746:755	arg1	characteristics					757:771	key mechanical characteristics	742:771	key mechanical characteristics such as dynamic yield stresses and high coverage	742:820	The ability to engineer key mechanical characteristics such as dynamic yield stresses and high coverage is shown, through systematic understanding of the composite mixture containing both gellan and λ-carrageenan.
34060150	3	1	theme	acute	475:479	arg1	syndrome					493:500	severe acute respiratory syndrome	468:500	severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)	468:527	This study reports on a nasal formulation with the capacity to combat such challenges, focusing on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
34060150	2	2	from	way	282:284	arg1	little					268:273	little	268:273	little	268:273	However, there is little in the way of adequate intervention that can protect an individual or prevent further spread.
34060150	6	3	theme	SARS-CoV-2	1011:1020	arg1	infection					1022:1030	SARS-CoV-2 infection	1011:1030	SARS-CoV-2 infection in Vero cells	1011:1044	Furthermore, the spray systems demonstrate highly potent capacities to prevent SARS-CoV-2 infection in Vero cells, resulting in complete inhibition when either treating, the cells, or the virus, prior to challenging for infection.
34060150	6	4	theme	complete	1060:1067	arg1	inhibition					1069:1078	complete inhibition	1060:1078	complete inhibition when either treating, the cells, or the virus, prior to challenging for infection	1060:1160	Furthermore, the spray systems demonstrate highly potent capacities to prevent SARS-CoV-2 infection in Vero cells, resulting in complete inhibition when either treating, the cells, or the virus, prior to challenging for infection.
34060150	6	5	from	infection	1022:1030	arg1	cells					1040:1044	Vero cells	1035:1044	Vero cells	1035:1044	Furthermore, the spray systems demonstrate highly potent capacities to prevent SARS-CoV-2 infection in Vero cells, resulting in complete inhibition when either treating, the cells, or the virus, prior to challenging for infection.
34060150	3	6	theme	respiratory	481:491	arg1	syndrome					493:500	severe acute respiratory syndrome	468:500	severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)	468:527	This study reports on a nasal formulation with the capacity to combat such challenges, focusing on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
34060150	4	7	theme	spray	568:572	arg1	Formulation					530:540	Formulation	530:540	Formulation of a polysaccharide-based spray, known for its mucoadhesive properties,	530:612	Formulation of a polysaccharide-based spray, known for its mucoadhesive properties, is undertaken and it is characterized for its mechanical, spray distribution, and antiviral properties.
34060150	0	8	theme	SARS-COV-2	93:102	arg1	Prophylaxis					78:88	Prophylaxis	78:88	Prophylaxis of SARS-COV-2	78:102	Formulation of a Composite Nasal Spray Enabling Enhanced Surface Coverage and Prophylaxis of SARS-COV-2.
34060150	0	8	theme	SARS-COV-2	93:102	arg1	Coverage					65:72	Enhanced Surface Coverage	48:72	Enhanced Surface Coverage	48:72	Formulation of a Composite Nasal Spray Enabling Enhanced Surface Coverage and Prophylaxis of SARS-COV-2.
34060150	8	9	theme	pathways	1515:1522	arg1	lining					1483:1488	the lining	1479:1488	the lining of the upper respiratory pathways	1479:1522	As such, a fully preventative spray is formulated, targeted at protecting the lining of the upper respiratory pathways against SARS-CoV-2.
34060150	5	10	theme	systematic	840:849	arg1	understanding					851:863	systematic understanding	840:863	systematic understanding of the composite mixture containing both gellan and λ-carrageenan	840:929	The ability to engineer key mechanical characteristics such as dynamic yield stresses and high coverage is shown, through systematic understanding of the composite mixture containing both gellan and λ-carrageenan.
34060150	4	11	theme	spray	672:676	arg1	distribution					678:689	spray distribution	672:689	spray distribution	672:689	Formulation of a polysaccharide-based spray, known for its mucoadhesive properties, is undertaken and it is characterized for its mechanical, spray distribution, and antiviral properties.
34060150	6	12	theme	treating	1092:1099	arg1	cells					1106:1110	the cells	1102:1110	the cells	1102:1110	Furthermore, the spray systems demonstrate highly potent capacities to prevent SARS-CoV-2 infection in Vero cells, resulting in complete inhibition when either treating, the cells, or the virus, prior to challenging for infection.
34060150	7	13	theme	virus	1302:1306	arg1	uptake					1308:1313	virus uptake	1302:1313	virus uptake into the cells	1302:1328	From this data, a mechanism for both prophylaxis and prevention is proposed; where entrapment within a polymeric coating sterically blocks virus uptake into the cells, inactivating the virus, and allowing clearance within the viscous medium.
34060150	1	14	theme	contraction	157:167	arg1	terms					143:147	terms	143:147	terms of both contraction and transmission	143:184	Airborne pathogens pose high risks in terms of both contraction and transmission within the respiratory pathways, particularly the nasal region.
34060150	8	15	theme	upper	1497:1501	arg1	pathways					1515:1522	the upper respiratory pathways	1493:1522	the upper respiratory pathways	1493:1522	As such, a fully preventative spray is formulated, targeted at protecting the lining of the upper respiratory pathways against SARS-CoV-2.
34060150	1	16	from	risks	134:138	arg1	terms					143:147	terms	143:147	terms of both contraction and transmission	143:184	Airborne pathogens pose high risks in terms of both contraction and transmission within the respiratory pathways, particularly the nasal region.
34060150	2	17	theme	intervention	298:309	arg1	way					282:284	the way	278:284	the way of adequate intervention that can protect an individual or prevent further spread	278:366	However, there is little in the way of adequate intervention that can protect an individual or prevent further spread.
34060150	1	18	theme	transmission	173:184	arg1	terms					143:147	terms	143:147	terms of both contraction and transmission	143:184	Airborne pathogens pose high risks in terms of both contraction and transmission within the respiratory pathways, particularly the nasal region.
34060150	6	19	theme	potent	982:987	arg1	capacities					989:998	highly potent capacities	975:998	highly potent capacities	975:998	Furthermore, the spray systems demonstrate highly potent capacities to prevent SARS-CoV-2 infection in Vero cells, resulting in complete inhibition when either treating, the cells, or the virus, prior to challenging for infection.
34060150	2	20	theme	adequate	289:296	arg1	intervention					298:309	adequate intervention	289:309	adequate intervention that can protect an individual or prevent further spread	289:366	However, there is little in the way of adequate intervention that can protect an individual or prevent further spread.
34060150	0	21	theme	Composite	17:25	arg1	Spray					33:37	a Composite Nasal Spray	15:37	a Composite Nasal Spray	15:37	Formulation of a Composite Nasal Spray Enabling Enhanced Surface Coverage and Prophylaxis of SARS-COV-2.
34060150	8	22	theme	respiratory	1503:1513	arg1	pathways					1515:1522	the upper respiratory pathways	1493:1522	the upper respiratory pathways	1493:1522	As such, a fully preventative spray is formulated, targeted at protecting the lining of the upper respiratory pathways against SARS-CoV-2.
34060150	5	23	theme	dynamic	781:787	arg1	stresses					795:802	dynamic yield stresses	781:802	dynamic yield stresses	781:802	The ability to engineer key mechanical characteristics such as dynamic yield stresses and high coverage is shown, through systematic understanding of the composite mixture containing both gellan and λ-carrageenan.
34060150	3	24	theme	such	439:442	arg1	challenges					444:453	such challenges	439:453	such challenges	439:453	This study reports on a nasal formulation with the capacity to combat such challenges, focusing on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
34060150	7	25	theme	viscous	1389:1395	arg1	medium					1397:1402	the viscous medium	1385:1402	the viscous medium	1385:1402	From this data, a mechanism for both prophylaxis and prevention is proposed; where entrapment within a polymeric coating sterically blocks virus uptake into the cells, inactivating the virus, and allowing clearance within the viscous medium.
34060150	4	26	theme	polysaccharide-based	547:566	arg1	spray					568:572	a polysaccharide-based spray	545:572	a polysaccharide-based spray	545:572	Formulation of a polysaccharide-based spray, known for its mucoadhesive properties, is undertaken and it is characterized for its mechanical, spray distribution, and antiviral properties.
34060150	2	27	theme	further	353:359	arg1	spread					361:366	further spread	353:366	further spread	353:366	However, there is little in the way of adequate intervention that can protect an individual or prevent further spread.
34060150	1	28	theme	Airborne	105:112	arg1	pathogens					114:122	Airborne pathogens	105:122	Airborne pathogens	105:122	Airborne pathogens pose high risks in terms of both contraction and transmission within the respiratory pathways, particularly the nasal region.
34060150	1	29	theme	respiratory	197:207	arg1	pathways					209:216	the respiratory pathways	193:216	the respiratory pathways	193:216	Airborne pathogens pose high risks in terms of both contraction and transmission within the respiratory pathways, particularly the nasal region.
34060150	0	30	theme	Spray	33:37	arg1	Formulation					0:10	Formulation	0:10	Formulation of a Composite Nasal Spray	0:37	Formulation of a Composite Nasal Spray Enabling Enhanced Surface Coverage and Prophylaxis of SARS-COV-2.
34060150	7	31	theme	polymeric	1266:1274	arg1	coating					1276:1282	a polymeric coating	1264:1282	a polymeric coating	1264:1282	From this data, a mechanism for both prophylaxis and prevention is proposed; where entrapment within a polymeric coating sterically blocks virus uptake into the cells, inactivating the virus, and allowing clearance within the viscous medium.
34060150	0	32	theme	Nasal	27:31	arg1	Spray					33:37	a Composite Nasal Spray	15:37	a Composite Nasal Spray	15:37	Formulation of a Composite Nasal Spray Enabling Enhanced Surface Coverage and Prophylaxis of SARS-COV-2.
34060150	6	33	theme	spray	949:953	arg1	systems					955:961	the spray systems	945:961	the spray systems	945:961	Furthermore, the spray systems demonstrate highly potent capacities to prevent SARS-CoV-2 infection in Vero cells, resulting in complete inhibition when either treating, the cells, or the virus, prior to challenging for infection.
34060150	3	34	theme	nasal	393:397	arg1	formulation					399:409	a nasal formulation	391:409	a nasal formulation with the capacity to combat such challenges	391:453	This study reports on a nasal formulation with the capacity to combat such challenges, focusing on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
34060150	8	35	theme	preventative	1422:1433	arg1	spray					1435:1439	a fully preventative spray	1414:1439	a fully preventative spray	1414:1439	As such, a fully preventative spray is formulated, targeted at protecting the lining of the upper respiratory pathways against SARS-CoV-2.
34060150	5	36	theme	high	808:811	arg1	coverage					813:820	high coverage	808:820	high coverage	808:820	The ability to engineer key mechanical characteristics such as dynamic yield stresses and high coverage is shown, through systematic understanding of the composite mixture containing both gellan and λ-carrageenan.
34060150	0	37	theme	Enhanced	48:55	arg1	Coverage					65:72	Enhanced Surface Coverage	48:72	Enhanced Surface Coverage	48:72	Formulation of a Composite Nasal Spray Enabling Enhanced Surface Coverage and Prophylaxis of SARS-COV-2.
34060150	5	38	theme	composite	872:880	arg1	mixture					882:888	the composite mixture	868:888	the composite mixture containing both gellan and λ-carrageenan	868:929	The ability to engineer key mechanical characteristics such as dynamic yield stresses and high coverage is shown, through systematic understanding of the composite mixture containing both gellan and λ-carrageenan.
34060150	3	39	with	formulation	399:409	arg1	capacity					420:427	the capacity to combat such challenges	416:453	the capacity to combat such challenges	416:453	This study reports on a nasal formulation with the capacity to combat such challenges, focusing on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
34060150	5	40	theme	key	742:744	arg1	coverage					813:820	high coverage	808:820	high coverage	808:820	The ability to engineer key mechanical characteristics such as dynamic yield stresses and high coverage is shown, through systematic understanding of the composite mixture containing both gellan and λ-carrageenan.
34060150	5	40	theme	key	742:744	arg1	stresses					795:802	dynamic yield stresses	781:802	dynamic yield stresses	781:802	The ability to engineer key mechanical characteristics such as dynamic yield stresses and high coverage is shown, through systematic understanding of the composite mixture containing both gellan and λ-carrageenan.
34060150	5	40	theme	key	742:744	arg1	characteristics					757:771	key mechanical characteristics	742:771	key mechanical characteristics such as dynamic yield stresses and high coverage	742:820	The ability to engineer key mechanical characteristics such as dynamic yield stresses and high coverage is shown, through systematic understanding of the composite mixture containing both gellan and λ-carrageenan.
34060150	5	41	theme	yield	789:793	arg1	stresses					795:802	dynamic yield stresses	781:802	dynamic yield stresses	781:802	The ability to engineer key mechanical characteristics such as dynamic yield stresses and high coverage is shown, through systematic understanding of the composite mixture containing both gellan and λ-carrageenan.
34060150	3	42	theme	severe	468:473	arg1	syndrome					493:500	severe acute respiratory syndrome	468:500	severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)	468:527	This study reports on a nasal formulation with the capacity to combat such challenges, focusing on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
34060150	2	43	from	little	268:273	arg1	way					282:284	the way	278:284	the way of adequate intervention that can protect an individual or prevent further spread	278:366	However, there is little in the way of adequate intervention that can protect an individual or prevent further spread.
34060150	7	44	dep	blocks	1295:1300	arg1	inactivating					1331:1342	inactivating	1331:1342	inactivating the virus	1331:1352	From this data, a mechanism for both prophylaxis and prevention is proposed; where entrapment within a polymeric coating sterically blocks virus uptake into the cells, inactivating the virus, and allowing clearance within the viscous medium.
34060150	7	44	dep	blocks	1295:1300	arg1	allowing					1359:1366	allowing	1359:1366	allowing clearance within the viscous medium	1359:1402	From this data, a mechanism for both prophylaxis and prevention is proposed; where entrapment within a polymeric coating sterically blocks virus uptake into the cells, inactivating the virus, and allowing clearance within the viscous medium.
34060150	1	45	dep	pathways	209:216	arg1	region					242:247	the nasal region	232:247	the nasal region	232:247	Airborne pathogens pose high risks in terms of both contraction and transmission within the respiratory pathways, particularly the nasal region.
34060150	1	46	theme	high	129:132	arg1	risks					134:138	high risks	129:138	high risks in terms of both contraction and transmission	129:184	Airborne pathogens pose high risks in terms of both contraction and transmission within the respiratory pathways, particularly the nasal region.
34060150	4	47	theme	mucoadhesive	589:600	arg1	properties					602:611	its mucoadhesive properties	585:611	its mucoadhesive properties	585:611	Formulation of a polysaccharide-based spray, known for its mucoadhesive properties, is undertaken and it is characterized for its mechanical, spray distribution, and antiviral properties.
34060150	4	48	theme	antiviral	696:704	arg1	properties					706:715	antiviral properties	696:715	antiviral properties	696:715	Formulation of a polysaccharide-based spray, known for its mucoadhesive properties, is undertaken and it is characterized for its mechanical, spray distribution, and antiviral properties.
34060150	6	49	theme	Vero	1035:1038	arg1	cells					1040:1044	Vero cells	1035:1044	Vero cells	1035:1044	Furthermore, the spray systems demonstrate highly potent capacities to prevent SARS-CoV-2 infection in Vero cells, resulting in complete inhibition when either treating, the cells, or the virus, prior to challenging for infection.
34060150	8	50	dep	formulated	1444:1453	arg1	targeted					1456:1463	targeted	1456:1463	targeted at protecting the lining of the upper respiratory pathways against SARS-CoV-2	1456:1541	As such, a fully preventative spray is formulated, targeted at protecting the lining of the upper respiratory pathways against SARS-CoV-2.
34060150	5	51	contain	containing	890:899	arg2	λ-carrageenan					917:929	λ-carrageenan	917:929	λ-carrageenan	917:929	The ability to engineer key mechanical characteristics such as dynamic yield stresses and high coverage is shown, through systematic understanding of the composite mixture containing both gellan and λ-carrageenan.
34060150	5	51	contain	containing	890:899	arg1	mixture					882:888	the composite mixture	868:888	the composite mixture containing both gellan and λ-carrageenan	868:929	The ability to engineer key mechanical characteristics such as dynamic yield stresses and high coverage is shown, through systematic understanding of the composite mixture containing both gellan and λ-carrageenan.
34060150	5	51	contain	containing	890:899	arg2	gellan					906:911	gellan	906:911	gellan	906:911	The ability to engineer key mechanical characteristics such as dynamic yield stresses and high coverage is shown, through systematic understanding of the composite mixture containing both gellan and λ-carrageenan.
34060150	3	52	theme	syndrome	493:500	arg1	SARS-CoV-2					517:526	SARS-CoV-2	517:526	SARS-CoV-2	517:526	This study reports on a nasal formulation with the capacity to combat such challenges, focusing on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
34060150	3	52	theme	syndrome	493:500	arg1	coronavirus-2					502:514	severe acute respiratory syndrome coronavirus-2	468:514	severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)	468:527	This study reports on a nasal formulation with the capacity to combat such challenges, focusing on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
34060150	1	53	theme	nasal	236:240	arg1	region					242:247	the nasal region	232:247	the nasal region	232:247	Airborne pathogens pose high risks in terms of both contraction and transmission within the respiratory pathways, particularly the nasal region.
34060150	0	54	theme	Surface	57:63	arg1	Coverage					65:72	Enhanced Surface Coverage	48:72	Enhanced Surface Coverage	48:72	Formulation of a Composite Nasal Spray Enabling Enhanced Surface Coverage and Prophylaxis of SARS-COV-2.
34060150	6	55	dep	virus	1120:1124	arg1	challenging					1136:1146	challenging	1136:1146	to challenging for infection	1133:1160	Furthermore, the spray systems demonstrate highly potent capacities to prevent SARS-CoV-2 infection in Vero cells, resulting in complete inhibition when either treating, the cells, or the virus, prior to challenging for infection.
34060150	5	56	theme	mixture	882:888	arg1	understanding					851:863	systematic understanding	840:863	systematic understanding of the composite mixture containing both gellan and λ-carrageenan	840:929	The ability to engineer key mechanical characteristics such as dynamic yield stresses and high coverage is shown, through systematic understanding of the composite mixture containing both gellan and λ-carrageenan.
32682974	0	0	theme	enhanced	64:71	arg1	reduction					83:91	enhanced catalytic reduction	64:91	enhanced catalytic reduction of organic dyes	64:107	Composites beads based on Fe3O4@MCM-41 and calcium alginate for enhanced catalytic reduction of organic dyes.
32682974	8	1	from	leaching	1314:1321	arg1	medium					1347:1352	the reaction medium	1334:1352	the reaction medium	1334:1352	Finally, the MC@CA(1) aerogel composite was reused in five successive cycles, with a slight loss of its activity during the fifth cycle due to the slight leaching of iron in the reaction medium.
32682974	5	2	from	immobilization	751:764	arg1	matrix					798:803	the alginate matrix	785:803	the alginate matrix leading to a high porous structure	785:838	The obtained results showed a higher immobilization of Fe3O4@MCM-41 in the alginate matrix leading to a high porous structure.
32682974	4	3	theme	shape	601:605	arg1	content					657:663	Fe3O4 content	651:663	Fe3O4 content	651:663	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	4	3	theme	shape	601:605	arg1	effect					587:592	effect	587:592	effect of the shape of composite beads	587:624	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	4	3	theme	shape	601:605	arg1	concentration					692:704	initial concentration	684:704	initial concentration of dye	684:711	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	4	3	theme	shape	601:605	arg1	concentration					627:639	concentration	627:639	concentration of NaBH4	627:648	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	4	3	theme	shape	601:605	arg1	mass					675:678	catalyst mass	666:678	catalyst mass	666:678	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	6	4	theme	MB	944:945	arg1	dye					947:949	MB dye	944:949	MB dye	944:949	The results showed that these catalysts have interesting catalytic activities towards the reduction of MB dye in which the reaction was more efficient with the catalyst containing a higher content of Fe3O4.
32682974	6	5	from	activities	908:917	arg1	reaction					964:971	the reaction	960:971	the reaction	960:971	The results showed that these catalysts have interesting catalytic activities towards the reduction of MB dye in which the reaction was more efficient with the catalyst containing a higher content of Fe3O4.
32682974	6	5	from	activities	908:917	arg1	efficient					982:990	efficient	982:990	efficient	982:990	The results showed that these catalysts have interesting catalytic activities towards the reduction of MB dye in which the reaction was more efficient with the catalyst containing a higher content of Fe3O4.
32682974	8	6	theme	@	1175:1175	arg1	composite					1190:1198	the MC@CA(1) aerogel composite	1169:1198	the MC@CA(1) aerogel composite	1169:1198	Finally, the MC@CA(1) aerogel composite was reused in five successive cycles, with a slight loss of its activity during the fifth cycle due to the slight leaching of iron in the reaction medium.
32682974	8	7	theme	successive	1219:1228	arg1	cycles					1230:1235	five successive cycles	1214:1235	five successive cycles	1214:1235	Finally, the MC@CA(1) aerogel composite was reused in five successive cycles, with a slight loss of its activity during the fifth cycle due to the slight leaching of iron in the reaction medium.
32682974	6	8	contain	have	881:884	arg1	catalysts					871:879	these catalysts	865:879	these catalysts	865:879	The results showed that these catalysts have interesting catalytic activities towards the reduction of MB dye in which the reaction was more efficient with the catalyst containing a higher content of Fe3O4.
32682974	6	8	contain	have	881:884	arg2	activities					908:917	interesting catalytic activities	886:917	interesting catalytic activities towards the reduction of MB dye in which the reaction was more efficient with the catalyst containing a higher content of Fe3O4	886:1045	The results showed that these catalysts have interesting catalytic activities towards the reduction of MB dye in which the reaction was more efficient with the catalyst containing a higher content of Fe3O4.
32682974	8	9	theme	activity	1264:1271	arg1	loss					1252:1255	a slight loss	1243:1255	a slight loss of its activity during the fifth cycle due to the slight leaching of iron in the reaction medium	1243:1352	Finally, the MC@CA(1) aerogel composite was reused in five successive cycles, with a slight loss of its activity during the fifth cycle due to the slight leaching of iron in the reaction medium.
32682974	8	10	theme	aerogel	1182:1188	arg1	composite					1190:1198	the MC@CA(1) aerogel composite	1169:1198	the MC@CA(1) aerogel composite	1169:1198	Finally, the MC@CA(1) aerogel composite was reused in five successive cycles, with a slight loss of its activity during the fifth cycle due to the slight leaching of iron in the reaction medium.
32682974	2	11	theme	@	245:245	arg1	matrix					370:375	a cross-linking matrix	354:375	a cross-linking matrix to give the composites MC@CA(x)	354:407	In the first step, the material Fe3O4@MCM-41 was prepared mechanically using several Fe3O4 contents, then was encapsulated by calcium alginate as a cross-linking matrix to give the composites MC@CA(x).
32682974	2	11	theme	@	245:245	arg1	MCM-41					246:251	the material Fe3O4@MCM-41	227:251	the material Fe3O4@MCM-41	227:251	In the first step, the material Fe3O4@MCM-41 was prepared mechanically using several Fe3O4 contents, then was encapsulated by calcium alginate as a cross-linking matrix to give the composites MC@CA(x).
32682974	3	12	from	reduction	461:469	arg1	system					501:506	a simple system	492:506	a simple system	492:506	The composite beads were used as catalysts for the reduction of MB and OG dyes in a simple system.
32682974	0	13	theme	catalytic	73:81	arg1	reduction					83:91	enhanced catalytic reduction	64:91	enhanced catalytic reduction of organic dyes	64:107	Composites beads based on Fe3O4@MCM-41 and calcium alginate for enhanced catalytic reduction of organic dyes.
32682974	8	14	theme	fifth	1284:1288	arg1	cycle					1290:1294	the fifth cycle	1280:1294	the fifth cycle	1280:1294	Finally, the MC@CA(1) aerogel composite was reused in five successive cycles, with a slight loss of its activity during the fifth cycle due to the slight leaching of iron in the reaction medium.
32682974	4	15	theme	Fe3O4	651:655	arg1	content					657:663	Fe3O4 content	651:663	Fe3O4 content	651:663	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	4	15	theme	Fe3O4	651:655	arg1	effect					587:592	effect	587:592	effect of the shape of composite beads	587:624	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	2	16	theme	Fe3O4	240:244	arg1	matrix					370:375	a cross-linking matrix	354:375	a cross-linking matrix to give the composites MC@CA(x)	354:407	In the first step, the material Fe3O4@MCM-41 was prepared mechanically using several Fe3O4 contents, then was encapsulated by calcium alginate as a cross-linking matrix to give the composites MC@CA(x).
32682974	2	16	theme	Fe3O4	240:244	arg1	MCM-41					246:251	the material Fe3O4@MCM-41	227:251	the material Fe3O4@MCM-41	227:251	In the first step, the material Fe3O4@MCM-41 was prepared mechanically using several Fe3O4 contents, then was encapsulated by calcium alginate as a cross-linking matrix to give the composites MC@CA(x).
32682974	4	17	theme	beads	620:624	arg1	shape					601:605	the shape	597:605	the shape of composite beads	597:624	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	0	18	theme	organic	96:102	arg1	dyes					104:107	organic dyes	96:107	organic dyes	96:107	Composites beads based on Fe3O4@MCM-41 and calcium alginate for enhanced catalytic reduction of organic dyes.
32682974	5	19	theme	alginate	789:796	arg1	matrix					798:803	the alginate matrix	785:803	the alginate matrix leading to a high porous structure	785:838	The obtained results showed a higher immobilization of Fe3O4@MCM-41 in the alginate matrix leading to a high porous structure.
32682974	2	20	theme	material	231:238	arg1	matrix					370:375	a cross-linking matrix	354:375	a cross-linking matrix to give the composites MC@CA(x)	354:407	In the first step, the material Fe3O4@MCM-41 was prepared mechanically using several Fe3O4 contents, then was encapsulated by calcium alginate as a cross-linking matrix to give the composites MC@CA(x).
32682974	2	20	theme	material	231:238	arg1	MCM-41					246:251	the material Fe3O4@MCM-41	227:251	the material Fe3O4@MCM-41	227:251	In the first step, the material Fe3O4@MCM-41 was prepared mechanically using several Fe3O4 contents, then was encapsulated by calcium alginate as a cross-linking matrix to give the composites MC@CA(x).
32682974	8	21	theme	CA	1176:1177	arg1	composite					1190:1198	the MC@CA(1) aerogel composite	1169:1198	the MC@CA(1) aerogel composite	1169:1198	Finally, the MC@CA(1) aerogel composite was reused in five successive cycles, with a slight loss of its activity during the fifth cycle due to the slight leaching of iron in the reaction medium.
32682974	1	22	theme	composite	148:156	arg1	beads					158:162	composite beads	148:162	composite beads based on calcium alginate and Fe3O4@MCM-41	148:205	This work concerns the preparation of composite beads based on calcium alginate and Fe3O4@MCM-41.
32682974	7	23	theme	CA	1055:1056	arg1	catalyst					1069:1076	The MC@CA(1) aerogel catalyst	1048:1076	The MC@CA(1) aerogel catalyst	1048:1076	The MC@CA(1) aerogel catalyst was the best in terms of stability compared to its MC@CA(1) hydrogel counterpart.
32682974	7	23	theme	CA	1055:1056	arg1	best					1086:1089	best	1086:1089	best	1086:1089	The MC@CA(1) aerogel catalyst was the best in terms of stability compared to its MC@CA(1) hydrogel counterpart.
32682974	6	24	with	efficient	982:990	arg1	catalyst					1001:1008	the catalyst	997:1008	the catalyst containing a higher content of Fe3O4	997:1045	The results showed that these catalysts have interesting catalytic activities towards the reduction of MB dye in which the reaction was more efficient with the catalyst containing a higher content of Fe3O4.
32682974	6	25	theme	Fe3O4	1041:1045	arg1	content					1030:1036	a higher content	1021:1036	a higher content of Fe3O4	1021:1045	The results showed that these catalysts have interesting catalytic activities towards the reduction of MB dye in which the reaction was more efficient with the catalyst containing a higher content of Fe3O4.
32682974	1	26	theme	beads	158:162	arg1	preparation					133:143	the preparation	129:143	the preparation of composite beads based on calcium alginate and Fe3O4@MCM-41	129:205	This work concerns the preparation of composite beads based on calcium alginate and Fe3O4@MCM-41.
32682974	2	27	theme	MC	400:401	arg1	CA					403:404	MC@CA	400:404	the composites MC@CA(x)	385:407	In the first step, the material Fe3O4@MCM-41 was prepared mechanically using several Fe3O4 contents, then was encapsulated by calcium alginate as a cross-linking matrix to give the composites MC@CA(x).
32682974	2	27	theme	MC	400:401	arg1	x					406:406	x	406:406	x	406:406	In the first step, the material Fe3O4@MCM-41 was prepared mechanically using several Fe3O4 contents, then was encapsulated by calcium alginate as a cross-linking matrix to give the composites MC@CA(x).
32682974	7	28	theme	aerogel	1061:1067	arg1	catalyst					1069:1076	The MC@CA(1) aerogel catalyst	1048:1076	The MC@CA(1) aerogel catalyst	1048:1076	The MC@CA(1) aerogel catalyst was the best in terms of stability compared to its MC@CA(1) hydrogel counterpart.
32682974	7	28	theme	aerogel	1061:1067	arg1	best					1086:1089	best	1086:1089	best	1086:1089	The MC@CA(1) aerogel catalyst was the best in terms of stability compared to its MC@CA(1) hydrogel counterpart.
32682974	8	29	from	iron	1326:1329	arg1	medium					1347:1352	the reaction medium	1334:1352	the reaction medium	1334:1352	Finally, the MC@CA(1) aerogel composite was reused in five successive cycles, with a slight loss of its activity during the fifth cycle due to the slight leaching of iron in the reaction medium.
32682974	8	30	theme	MC	1173:1174	arg1	composite					1190:1198	the MC@CA(1) aerogel composite	1169:1198	the MC@CA(1) aerogel composite	1169:1198	Finally, the MC@CA(1) aerogel composite was reused in five successive cycles, with a slight loss of its activity during the fifth cycle due to the slight leaching of iron in the reaction medium.
32682974	7	31	theme	stability	1103:1111	arg1	terms					1094:1098	terms	1094:1098	terms of stability	1094:1111	The MC@CA(1) aerogel catalyst was the best in terms of stability compared to its MC@CA(1) hydrogel counterpart.
32682974	2	32	theme	calcium	334:340	arg1	alginate					342:349	calcium alginate	334:349	calcium alginate	334:349	In the first step, the material Fe3O4@MCM-41 was prepared mechanically using several Fe3O4 contents, then was encapsulated by calcium alginate as a cross-linking matrix to give the composites MC@CA(x).
32682974	0	33	theme	Composites	0:9	arg1	beads					11:15	Composites beads	0:15	Composites beads	0:15	Composites beads based on Fe3O4@MCM-41 and calcium alginate for enhanced catalytic reduction of organic dyes.
32682974	2	34	dep	composites	389:398	arg1	CA					403:404	MC@CA	400:404	the composites MC@CA(x)	385:407	In the first step, the material Fe3O4@MCM-41 was prepared mechanically using several Fe3O4 contents, then was encapsulated by calcium alginate as a cross-linking matrix to give the composites MC@CA(x).
32682974	2	34	dep	composites	389:398	arg1	x					406:406	x	406:406	x	406:406	In the first step, the material Fe3O4@MCM-41 was prepared mechanically using several Fe3O4 contents, then was encapsulated by calcium alginate as a cross-linking matrix to give the composites MC@CA(x).
32682974	5	35	theme	higher	744:749	arg1	immobilization					751:764	a higher immobilization	742:764	a higher immobilization of Fe3O4@MCM-41 in the alginate matrix leading to a high porous structure	742:838	The obtained results showed a higher immobilization of Fe3O4@MCM-41 in the alginate matrix leading to a high porous structure.
32682974	3	36	theme	OG	481:482	arg1	dyes					484:487	OG dyes	481:487	OG dyes	481:487	The composite beads were used as catalysts for the reduction of MB and OG dyes in a simple system.
32682974	7	37	theme	MC	1129:1130	arg1	counterpart					1147:1157	its MC@CA(1) hydrogel counterpart	1125:1157	its MC@CA(1) hydrogel counterpart	1125:1157	The MC@CA(1) aerogel catalyst was the best in terms of stability compared to its MC@CA(1) hydrogel counterpart.
32682974	5	38	theme	Fe3O4	769:773	arg1	MCM-41					775:780	Fe3O4@MCM-41	769:780	Fe3O4@MCM-41	769:780	The obtained results showed a higher immobilization of Fe3O4@MCM-41 in the alginate matrix leading to a high porous structure.
32682974	5	39	theme	porous	823:828	arg1	structure					830:838	a high porous structure	816:838	a high porous structure	816:838	The obtained results showed a higher immobilization of Fe3O4@MCM-41 in the alginate matrix leading to a high porous structure.
32682974	1	40	theme	calcium	173:179	arg1	alginate					181:188	calcium alginate	173:188	calcium alginate	173:188	This work concerns the preparation of composite beads based on calcium alginate and Fe3O4@MCM-41.
32682974	5	41	theme	high	818:821	arg1	structure					830:838	a high porous structure	816:838	a high porous structure	816:838	The obtained results showed a higher immobilization of Fe3O4@MCM-41 in the alginate matrix leading to a high porous structure.
32682974	0	42	theme	dyes	104:107	arg1	reduction					83:91	enhanced catalytic reduction	64:91	enhanced catalytic reduction of organic dyes	64:107	Composites beads based on Fe3O4@MCM-41 and calcium alginate for enhanced catalytic reduction of organic dyes.
32682974	6	43	theme	interesting	886:896	arg1	activities					908:917	interesting catalytic activities	886:917	interesting catalytic activities towards the reduction of MB dye in which the reaction was more efficient with the catalyst containing a higher content of Fe3O4	886:1045	The results showed that these catalysts have interesting catalytic activities towards the reduction of MB dye in which the reaction was more efficient with the catalyst containing a higher content of Fe3O4.
32682974	3	44	theme	MB	474:475	arg1	reduction					461:469	the reduction	457:469	the reduction of MB and OG dyes in a simple system	457:506	The composite beads were used as catalysts for the reduction of MB and OG dyes in a simple system.
32682974	8	45	theme	slight	1245:1250	arg1	loss					1252:1255	a slight loss	1243:1255	a slight loss of its activity during the fifth cycle due to the slight leaching of iron in the reaction medium	1243:1352	Finally, the MC@CA(1) aerogel composite was reused in five successive cycles, with a slight loss of its activity during the fifth cycle due to the slight leaching of iron in the reaction medium.
32682974	7	46	theme	CA	1132:1133	arg1	counterpart					1147:1157	its MC@CA(1) hydrogel counterpart	1125:1157	its MC@CA(1) hydrogel counterpart	1125:1157	The MC@CA(1) aerogel catalyst was the best in terms of stability compared to its MC@CA(1) hydrogel counterpart.
32682974	0	47	theme	@	31:31	arg1	MCM-41					32:37	Fe3O4@MCM-41	26:37	Fe3O4@MCM-41	26:37	Composites beads based on Fe3O4@MCM-41 and calcium alginate for enhanced catalytic reduction of organic dyes.
32682974	3	48	theme	composite	414:422	arg1	catalysts					443:451	catalysts	443:451	catalysts	443:451	The composite beads were used as catalysts for the reduction of MB and OG dyes in a simple system.
32682974	3	48	theme	composite	414:422	arg1	beads					424:428	The composite beads	410:428	The composite beads	410:428	The composite beads were used as catalysts for the reduction of MB and OG dyes in a simple system.
32682974	8	49	from	medium	1347:1352	arg1	leaching					1314:1321	the slight leaching	1303:1321	the slight leaching of iron in the reaction medium	1303:1352	Finally, the MC@CA(1) aerogel composite was reused in five successive cycles, with a slight loss of its activity during the fifth cycle due to the slight leaching of iron in the reaction medium.
32682974	7	50	theme	MC	1052:1053	arg1	catalyst					1069:1076	The MC@CA(1) aerogel catalyst	1048:1076	The MC@CA(1) aerogel catalyst	1048:1076	The MC@CA(1) aerogel catalyst was the best in terms of stability compared to its MC@CA(1) hydrogel counterpart.
32682974	7	50	theme	MC	1052:1053	arg1	best					1086:1089	best	1086:1089	best	1086:1089	The MC@CA(1) aerogel catalyst was the best in terms of stability compared to its MC@CA(1) hydrogel counterpart.
32682974	4	51	theme	dye	709:711	arg1	content					657:663	Fe3O4 content	651:663	Fe3O4 content	651:663	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	4	51	theme	dye	709:711	arg1	concentration					627:639	concentration	627:639	concentration of NaBH4	627:648	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	4	51	theme	dye	709:711	arg1	concentration					692:704	initial concentration	684:704	initial concentration of dye	684:711	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	4	51	theme	dye	709:711	arg1	mass					675:678	catalyst mass	666:678	catalyst mass	666:678	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	4	51	theme	dye	709:711	arg1	effect					587:592	effect	587:592	effect of the shape of composite beads	587:624	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	6	52	theme	dye	947:949	arg1	reduction					931:939	the reduction	927:939	the reduction of MB dye	927:949	The results showed that these catalysts have interesting catalytic activities towards the reduction of MB dye in which the reaction was more efficient with the catalyst containing a higher content of Fe3O4.
32682974	2	53	theme	cross-linking	356:368	arg1	matrix					370:375	a cross-linking matrix	354:375	a cross-linking matrix to give the composites MC@CA(x)	354:407	In the first step, the material Fe3O4@MCM-41 was prepared mechanically using several Fe3O4 contents, then was encapsulated by calcium alginate as a cross-linking matrix to give the composites MC@CA(x).
32682974	2	53	theme	cross-linking	356:368	arg1	MCM-41					246:251	the material Fe3O4@MCM-41	227:251	the material Fe3O4@MCM-41	227:251	In the first step, the material Fe3O4@MCM-41 was prepared mechanically using several Fe3O4 contents, then was encapsulated by calcium alginate as a cross-linking matrix to give the composites MC@CA(x).
32682974	0	54	theme	Fe3O4	26:30	arg1	MCM-41					32:37	Fe3O4@MCM-41	26:37	Fe3O4@MCM-41	26:37	Composites beads based on Fe3O4@MCM-41 and calcium alginate for enhanced catalytic reduction of organic dyes.
32682974	8	55	theme	iron	1326:1329	arg1	leaching					1314:1321	the slight leaching	1303:1321	the slight leaching of iron in the reaction medium	1303:1352	Finally, the MC@CA(1) aerogel composite was reused in five successive cycles, with a slight loss of its activity during the fifth cycle due to the slight leaching of iron in the reaction medium.
32682974	3	56	theme	simple	494:499	arg1	system					501:506	a simple system	492:506	a simple system	492:506	The composite beads were used as catalysts for the reduction of MB and OG dyes in a simple system.
32682974	1	57	theme	Fe3O4	194:198	arg1	MCM-41					200:205	Fe3O4@MCM-41	194:205	Fe3O4@MCM-41	194:205	This work concerns the preparation of composite beads based on calcium alginate and Fe3O4@MCM-41.
32682974	6	58	contain	containing	1010:1019	arg1	catalyst					1001:1008	the catalyst	997:1008	the catalyst containing a higher content of Fe3O4	997:1045	The results showed that these catalysts have interesting catalytic activities towards the reduction of MB dye in which the reaction was more efficient with the catalyst containing a higher content of Fe3O4.
32682974	6	58	contain	containing	1010:1019	arg2	content					1030:1036	a higher content	1021:1036	a higher content of Fe3O4	1021:1045	The results showed that these catalysts have interesting catalytic activities towards the reduction of MB dye in which the reaction was more efficient with the catalyst containing a higher content of Fe3O4.
32682974	3	59	theme	dyes	484:487	arg1	reduction					461:469	the reduction	457:469	the reduction of MB and OG dyes in a simple system	457:506	The composite beads were used as catalysts for the reduction of MB and OG dyes in a simple system.
32682974	4	60	theme	composite	610:618	arg1	beads					620:624	composite beads	610:624	composite beads	610:624	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	2	61	theme	@	402:402	arg1	CA					403:404	MC@CA	400:404	the composites MC@CA(x)	385:407	In the first step, the material Fe3O4@MCM-41 was prepared mechanically using several Fe3O4 contents, then was encapsulated by calcium alginate as a cross-linking matrix to give the composites MC@CA(x).
32682974	2	61	theme	@	402:402	arg1	x					406:406	x	406:406	x	406:406	In the first step, the material Fe3O4@MCM-41 was prepared mechanically using several Fe3O4 contents, then was encapsulated by calcium alginate as a cross-linking matrix to give the composites MC@CA(x).
32682974	8	62	theme	slight	1307:1312	arg1	leaching					1314:1321	the slight leaching	1303:1321	the slight leaching of iron in the reaction medium	1303:1352	Finally, the MC@CA(1) aerogel composite was reused in five successive cycles, with a slight loss of its activity during the fifth cycle due to the slight leaching of iron in the reaction medium.
32682974	7	63	theme	hydrogel	1138:1145	arg1	counterpart					1147:1157	its MC@CA(1) hydrogel counterpart	1125:1157	its MC@CA(1) hydrogel counterpart	1125:1157	The MC@CA(1) aerogel catalyst was the best in terms of stability compared to its MC@CA(1) hydrogel counterpart.
32682974	2	64	theme	Fe3O4	293:297	arg1	contents					299:306	several Fe3O4 contents	285:306	several Fe3O4 contents	285:306	In the first step, the material Fe3O4@MCM-41 was prepared mechanically using several Fe3O4 contents, then was encapsulated by calcium alginate as a cross-linking matrix to give the composites MC@CA(x).
32682974	4	65	theme	initial	684:690	arg1	effect					587:592	effect	587:592	effect of the shape of composite beads	587:624	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	4	65	theme	initial	684:690	arg1	concentration					692:704	initial concentration	684:704	initial concentration of dye	684:711	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	2	66	theme	several	285:291	arg1	contents					299:306	several Fe3O4 contents	285:306	several Fe3O4 contents	285:306	In the first step, the material Fe3O4@MCM-41 was prepared mechanically using several Fe3O4 contents, then was encapsulated by calcium alginate as a cross-linking matrix to give the composites MC@CA(x).
32682974	6	67	theme	higher	1023:1028	arg1	content					1030:1036	a higher content	1021:1036	a higher content of Fe3O4	1021:1045	The results showed that these catalysts have interesting catalytic activities towards the reduction of MB dye in which the reaction was more efficient with the catalyst containing a higher content of Fe3O4.
32682974	1	68	theme	@	199:199	arg1	MCM-41					200:205	Fe3O4@MCM-41	194:205	Fe3O4@MCM-41	194:205	This work concerns the preparation of composite beads based on calcium alginate and Fe3O4@MCM-41.
32682974	4	69	theme	catalyst	666:673	arg1	mass					675:678	catalyst mass	666:678	catalyst mass	666:678	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	4	69	theme	catalyst	666:673	arg1	effect					587:592	effect	587:592	effect of the shape of composite beads	587:624	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	4	70	theme	NaBH4	644:648	arg1	content					657:663	Fe3O4 content	651:663	Fe3O4 content	651:663	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	4	70	theme	NaBH4	644:648	arg1	concentration					627:639	concentration	627:639	concentration of NaBH4	627:648	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	4	70	theme	NaBH4	644:648	arg1	concentration					692:704	initial concentration	684:704	initial concentration of dye	684:711	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	4	70	theme	NaBH4	644:648	arg1	mass					675:678	catalyst mass	666:678	catalyst mass	666:678	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	4	70	theme	NaBH4	644:648	arg1	effect					587:592	effect	587:592	effect of the shape of composite beads	587:624	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	2	71	dep	prepared	257:264	arg1	encapsulated					318:329	encapsulated	318:329	was encapsulated by calcium alginate as a cross-linking matrix to give the composites MC@CA(x)	314:407	In the first step, the material Fe3O4@MCM-41 was prepared mechanically using several Fe3O4 contents, then was encapsulated by calcium alginate as a cross-linking matrix to give the composites MC@CA(x).
32682974	8	72	theme	reaction	1338:1345	arg1	medium					1347:1352	the reaction medium	1334:1352	the reaction medium	1334:1352	Finally, the MC@CA(1) aerogel composite was reused in five successive cycles, with a slight loss of its activity during the fifth cycle due to the slight leaching of iron in the reaction medium.
32682974	4	73	theme	reduction	542:550	arg1	reaction					552:559	the reduction reaction	538:559	the reduction reaction	538:559	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32682974	2	74	theme	first	215:219	arg1	step					221:224	the first step	211:224	the first step	211:224	In the first step, the material Fe3O4@MCM-41 was prepared mechanically using several Fe3O4 contents, then was encapsulated by calcium alginate as a cross-linking matrix to give the composites MC@CA(x).
32682974	5	75	theme	obtained	718:725	arg1	results					727:733	The obtained results	714:733	The obtained results	714:733	The obtained results showed a higher immobilization of Fe3O4@MCM-41 in the alginate matrix leading to a high porous structure.
32682974	5	76	theme	@	774:774	arg1	MCM-41					775:780	Fe3O4@MCM-41	769:780	Fe3O4@MCM-41	769:780	The obtained results showed a higher immobilization of Fe3O4@MCM-41 in the alginate matrix leading to a high porous structure.
32682974	7	77	theme	@	1131:1131	arg1	counterpart					1147:1157	its MC@CA(1) hydrogel counterpart	1125:1157	its MC@CA(1) hydrogel counterpart	1125:1157	The MC@CA(1) aerogel catalyst was the best in terms of stability compared to its MC@CA(1) hydrogel counterpart.
32682974	6	78	theme	catalytic	898:906	arg1	activities					908:917	interesting catalytic activities	886:917	interesting catalytic activities towards the reduction of MB dye in which the reaction was more efficient with the catalyst containing a higher content of Fe3O4	886:1045	The results showed that these catalysts have interesting catalytic activities towards the reduction of MB dye in which the reaction was more efficient with the catalyst containing a higher content of Fe3O4.
32682974	0	79	theme	calcium	43:49	arg1	alginate					51:58	calcium alginate	43:58	calcium alginate	43:58	Composites beads based on Fe3O4@MCM-41 and calcium alginate for enhanced catalytic reduction of organic dyes.
32682974	7	80	theme	@	1054:1054	arg1	catalyst					1069:1076	The MC@CA(1) aerogel catalyst	1048:1076	The MC@CA(1) aerogel catalyst	1048:1076	The MC@CA(1) aerogel catalyst was the best in terms of stability compared to its MC@CA(1) hydrogel counterpart.
32682974	7	80	theme	@	1054:1054	arg1	best					1086:1089	best	1086:1089	best	1086:1089	The MC@CA(1) aerogel catalyst was the best in terms of stability compared to its MC@CA(1) hydrogel counterpart.
32682974	3	81	used	used	435:438	arg2	catalysts					443:451	catalysts	443:451	catalysts	443:451	The composite beads were used as catalysts for the reduction of MB and OG dyes in a simple system.
32682974	3	81	used	used	435:438	arg2	beads					424:428	The composite beads	410:428	The composite beads	410:428	The composite beads were used as catalysts for the reduction of MB and OG dyes in a simple system.
32682974	6	82	from	efficient	982:990	arg1	activities					908:917	interesting catalytic activities	886:917	interesting catalytic activities towards the reduction of MB dye in which the reaction was more efficient with the catalyst containing a higher content of Fe3O4	886:1045	The results showed that these catalysts have interesting catalytic activities towards the reduction of MB dye in which the reaction was more efficient with the catalyst containing a higher content of Fe3O4.
32682974	5	83	theme	MCM-41	775:780	arg1	immobilization					751:764	a higher immobilization	742:764	a higher immobilization of Fe3O4@MCM-41 in the alginate matrix leading to a high porous structure	742:838	The obtained results showed a higher immobilization of Fe3O4@MCM-41 in the alginate matrix leading to a high porous structure.
32682974	4	84	theme	Several	509:515	arg1	parameters					517:526	Several parameters	509:526	Several parameters affecting the reduction reaction	509:559	Several parameters affecting the reduction reaction were investigated such as effect of the shape of composite beads, concentration of NaBH4, Fe3O4 content, catalyst mass and initial concentration of dye.
32829794	5	0	theme	wound	1243:1247	arg1	healing					1249:1255	wound healing	1243:1255	wound healing applications	1243:1268	Finite element analysis simulation was employed to further advance the calculation accuracy of the nanoindentation tests, concluding that combination of an experimental and a numerical technique may constitute a useful method to characterize the mechanical behavior of composite hydrogel films for use in wound healing applications.
32829794	3	1	theme	hydrogels	695:703	arg1	viscosity					639:647	viscosity	639:647	viscosity	639:647	The effect of the bioactive compounds on the viscosity and mechanical properties of the 3D printable hydrogels was assessed with rheological, nanoindentation and shear test measurements.
32829794	3	1	theme	hydrogels	695:703	arg1	properties					664:673	mechanical properties	653:673	mechanical properties	653:673	The effect of the bioactive compounds on the viscosity and mechanical properties of the 3D printable hydrogels was assessed with rheological, nanoindentation and shear test measurements.
32829794	1	2	theme	comprehensive	169:181	arg1	evaluation					202:211	the comprehensive physico-mechanical evaluation	165:211	the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach	165:393	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	0	3	theme	inks	99:102	arg1	Physico-mechanical					0:17	Physico-mechanical	0:17	Physico-mechanical	0:17	Physico-mechanical and finite element analysis evaluation of 3D printable alginate-methylcellulose inks for wound healing applications.
32829794	0	3	theme	inks	99:102	arg1	analysis					38:45	finite element analysis	23:45	finite element analysis	23:45	Physico-mechanical and finite element analysis evaluation of 3D printable alginate-methylcellulose inks for wound healing applications.
32829794	5	4	theme	healing	1249:1255	arg1	applications					1257:1268	wound healing applications	1243:1268	wound healing applications	1243:1268	Finite element analysis simulation was employed to further advance the calculation accuracy of the nanoindentation tests, concluding that combination of an experimental and a numerical technique may constitute a useful method to characterize the mechanical behavior of composite hydrogel films for use in wound healing applications.
32829794	1	5	theme	physico-mechanical	183:200	arg1	evaluation					202:211	the comprehensive physico-mechanical evaluation	165:211	the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach	165:393	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	1	6	theme	aloe	305:308	arg1	honey					298:302	Manuka honey	291:302	Manuka honey	291:302	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	1	6	theme	aloe	305:308	arg1	gel					315:317	aloe vera gel	305:317	aloe vera gel	305:317	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	0	7	theme	healing	114:120	arg1	applications					122:133	wound healing applications	108:133	wound healing applications	108:133	Physico-mechanical and finite element analysis evaluation of 3D printable alginate-methylcellulose inks for wound healing applications.
32829794	3	8	theme	compounds	622:630	arg1	effect					598:603	The effect	594:603	The effect of the bioactive compounds on the viscosity and mechanical properties of the 3D printable hydrogels	594:703	The effect of the bioactive compounds on the viscosity and mechanical properties of the 3D printable hydrogels was assessed with rheological, nanoindentation and shear test measurements.
32829794	1	9	theme	vera	310:313	arg1	honey					298:302	Manuka honey	291:302	Manuka honey	291:302	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	1	9	theme	vera	310:313	arg1	gel					315:317	aloe vera gel	305:317	aloe vera gel	305:317	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	0	10	theme	wound	108:112	arg1	healing					114:120	wound healing	108:120	wound healing applications	108:133	Physico-mechanical and finite element analysis evaluation of 3D printable alginate-methylcellulose inks for wound healing applications.
32829794	5	11	theme	hydrogel	1217:1224	arg1	films					1226:1230	composite hydrogel films	1207:1230	composite hydrogel films	1207:1230	Finite element analysis simulation was employed to further advance the calculation accuracy of the nanoindentation tests, concluding that combination of an experimental and a numerical technique may constitute a useful method to characterize the mechanical behavior of composite hydrogel films for use in wound healing applications.
32829794	3	12	from	effect	598:603	arg1	viscosity					639:647	viscosity	639:647	viscosity	639:647	The effect of the bioactive compounds on the viscosity and mechanical properties of the 3D printable hydrogels was assessed with rheological, nanoindentation and shear test measurements.
32829794	3	12	from	effect	598:603	arg1	properties					664:673	mechanical properties	653:673	mechanical properties	653:673	The effect of the bioactive compounds on the viscosity and mechanical properties of the 3D printable hydrogels was assessed with rheological, nanoindentation and shear test measurements.
32829794	2	13	theme	printable	403:411	arg1	inks					426:429	The 3D printable carbohydrate inks	396:429	The 3D printable carbohydrate inks	396:429	The 3D printable carbohydrate inks demonstrated good swelling properties under moist conditions and adequate antimicrobial and antibiofilm efficacy against both Gram positive and negative bacteria.
32829794	3	14	dep	measurements	767:778	arg1	nanoindentation					736:750	nanoindentation	736:750	nanoindentation	736:750	The effect of the bioactive compounds on the viscosity and mechanical properties of the 3D printable hydrogels was assessed with rheological, nanoindentation and shear test measurements.
32829794	3	14	dep	measurements	767:778	arg1	test					762:765	shear test	756:765	shear test	756:765	The effect of the bioactive compounds on the viscosity and mechanical properties of the 3D printable hydrogels was assessed with rheological, nanoindentation and shear test measurements.
32829794	3	14	dep	measurements	767:778	arg1	measurements					767:778	rheological, nanoindentation and shear test measurements	723:778	measurements	767:778	The effect of the bioactive compounds on the viscosity and mechanical properties of the 3D printable hydrogels was assessed with rheological, nanoindentation and shear test measurements.
32829794	4	15	theme	hydrogel	785:792	arg1	compositions					794:805	All hydrogel compositions	781:805	All hydrogel compositions	781:805	All hydrogel compositions showed good biocompatibility on human dermal fibroblasts, stimulating cell growth as confirmed by an in vitro wound healing assay.
32829794	4	16	theme	human	839:843	arg1	fibroblasts					852:862	human dermal fibroblasts	839:862	human dermal fibroblasts	839:862	All hydrogel compositions showed good biocompatibility on human dermal fibroblasts, stimulating cell growth as confirmed by an in vitro wound healing assay.
32829794	5	17	theme	Finite	938:943	arg1	simulation					962:971	Finite element analysis simulation	938:971	Finite element analysis simulation	938:971	Finite element analysis simulation was employed to further advance the calculation accuracy of the nanoindentation tests, concluding that combination of an experimental and a numerical technique may constitute a useful method to characterize the mechanical behavior of composite hydrogel films for use in wound healing applications.
32829794	2	18	theme	good	444:447	arg1	properties					458:467	good swelling properties	444:467	good swelling properties under moist conditions	444:490	The 3D printable carbohydrate inks demonstrated good swelling properties under moist conditions and adequate antimicrobial and antibiofilm efficacy against both Gram positive and negative bacteria.
32829794	4	19	dep	in	908:909	arg1	vitro					911:915	vitro	911:915	vitro	911:915	All hydrogel compositions showed good biocompatibility on human dermal fibroblasts, stimulating cell growth as confirmed by an in vitro wound healing assay.
32829794	5	20	theme	calculation	1009:1019	arg1	accuracy					1021:1028	the calculation accuracy	1005:1028	the calculation accuracy of the nanoindentation tests	1005:1057	Finite element analysis simulation was employed to further advance the calculation accuracy of the nanoindentation tests, concluding that combination of an experimental and a numerical technique may constitute a useful method to characterize the mechanical behavior of composite hydrogel films for use in wound healing applications.
32829794	4	21	theme	in	908:909	arg1	healing					923:929	an in vitro wound healing	905:929	an in vitro wound healing assay	905:935	All hydrogel compositions showed good biocompatibility on human dermal fibroblasts, stimulating cell growth as confirmed by an in vitro wound healing assay.
32829794	3	22	theme	bioactive	612:620	arg1	compounds					622:630	the bioactive compounds	608:630	the bioactive compounds	608:630	The effect of the bioactive compounds on the viscosity and mechanical properties of the 3D printable hydrogels was assessed with rheological, nanoindentation and shear test measurements.
32829794	2	23	theme	swelling	449:456	arg1	properties					458:467	good swelling properties	444:467	good swelling properties under moist conditions	444:490	The 3D printable carbohydrate inks demonstrated good swelling properties under moist conditions and adequate antimicrobial and antibiofilm efficacy against both Gram positive and negative bacteria.
32829794	2	24	theme	antibiofilm	523:533	arg1	efficacy					535:542	antibiofilm efficacy	523:542	antibiofilm efficacy	523:542	The 3D printable carbohydrate inks demonstrated good swelling properties under moist conditions and adequate antimicrobial and antibiofilm efficacy against both Gram positive and negative bacteria.
32829794	2	25	theme	positive	562:569	arg1	bacteria					584:591	both Gram positive and negative bacteria	552:591	both Gram positive and negative bacteria	552:591	The 3D printable carbohydrate inks demonstrated good swelling properties under moist conditions and adequate antimicrobial and antibiofilm efficacy against both Gram positive and negative bacteria.
32829794	5	26	theme	numerical	1113:1121	arg1	technique					1123:1131	a numerical technique	1111:1131	a numerical technique	1111:1131	Finite element analysis simulation was employed to further advance the calculation accuracy of the nanoindentation tests, concluding that combination of an experimental and a numerical technique may constitute a useful method to characterize the mechanical behavior of composite hydrogel films for use in wound healing applications.
32829794	4	27	theme	dermal	845:850	arg1	fibroblasts					852:862	human dermal fibroblasts	839:862	human dermal fibroblasts	839:862	All hydrogel compositions showed good biocompatibility on human dermal fibroblasts, stimulating cell growth as confirmed by an in vitro wound healing assay.
32829794	5	28	theme	films	1226:1230	arg1	behavior					1195:1202	the mechanical behavior	1180:1202	the mechanical behavior of composite hydrogel films for use in wound healing applications	1180:1268	Finite element analysis simulation was employed to further advance the calculation accuracy of the nanoindentation tests, concluding that combination of an experimental and a numerical technique may constitute a useful method to characterize the mechanical behavior of composite hydrogel films for use in wound healing applications.
32829794	1	29	theme	3D	216:217	arg1	hydrogels					254:262	3D printable alginate-methylcellulose hydrogels	216:262	3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil)	216:344	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	5	30	from	use	1236:1238	arg1	applications					1257:1268	wound healing applications	1243:1268	wound healing applications	1243:1268	Finite element analysis simulation was employed to further advance the calculation accuracy of the nanoindentation tests, concluding that combination of an experimental and a numerical technique may constitute a useful method to characterize the mechanical behavior of composite hydrogel films for use in wound healing applications.
32829794	3	31	theme	mechanical	653:662	arg1	properties					664:673	mechanical properties	653:673	mechanical properties	653:673	The effect of the bioactive compounds on the viscosity and mechanical properties of the 3D printable hydrogels was assessed with rheological, nanoindentation and shear test measurements.
32829794	1	32	theme	printable	219:227	arg1	hydrogels					254:262	3D printable alginate-methylcellulose hydrogels	216:262	3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil)	216:344	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	0	33	theme	element	30:36	arg1	analysis					38:45	finite element analysis	23:45	finite element analysis	23:45	Physico-mechanical and finite element analysis evaluation of 3D printable alginate-methylcellulose inks for wound healing applications.
32829794	1	34	theme	eucalyptus	320:329	arg1	oil					341:343	eucalyptus essential oil	320:343	eucalyptus essential oil	320:343	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	1	34	theme	eucalyptus	320:329	arg1	honey					298:302	Manuka honey	291:302	Manuka honey	291:302	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	5	35	theme	composite	1207:1215	arg1	films					1226:1230	composite hydrogel films	1207:1230	composite hydrogel films	1207:1230	Finite element analysis simulation was employed to further advance the calculation accuracy of the nanoindentation tests, concluding that combination of an experimental and a numerical technique may constitute a useful method to characterize the mechanical behavior of composite hydrogel films for use in wound healing applications.
32829794	5	36	theme	nanoindentation	1037:1051	arg1	tests					1053:1057	the nanoindentation tests	1033:1057	the nanoindentation tests	1033:1057	Finite element analysis simulation was employed to further advance the calculation accuracy of the nanoindentation tests, concluding that combination of an experimental and a numerical technique may constitute a useful method to characterize the mechanical behavior of composite hydrogel films for use in wound healing applications.
32829794	1	37	theme	alginate-methylcellulose	229:252	arg1	hydrogels					254:262	3D printable alginate-methylcellulose hydrogels	216:262	3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil)	216:344	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	0	38	theme	finite	23:28	arg1	analysis					38:45	finite element analysis	23:45	finite element analysis	23:45	Physico-mechanical and finite element analysis evaluation of 3D printable alginate-methylcellulose inks for wound healing applications.
32829794	1	39	theme	essential	331:339	arg1	oil					341:343	eucalyptus essential oil	320:343	eucalyptus essential oil	320:343	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	1	39	theme	essential	331:339	arg1	honey					298:302	Manuka honey	291:302	Manuka honey	291:302	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	5	40	theme	mechanical	1184:1193	arg1	behavior					1195:1202	the mechanical behavior	1180:1202	the mechanical behavior of composite hydrogel films for use in wound healing applications	1180:1268	Finite element analysis simulation was employed to further advance the calculation accuracy of the nanoindentation tests, concluding that combination of an experimental and a numerical technique may constitute a useful method to characterize the mechanical behavior of composite hydrogel films for use in wound healing applications.
32829794	2	41	theme	3D	400:401	arg1	inks					426:429	The 3D printable carbohydrate inks	396:429	The 3D printable carbohydrate inks	396:429	The 3D printable carbohydrate inks demonstrated good swelling properties under moist conditions and adequate antimicrobial and antibiofilm efficacy against both Gram positive and negative bacteria.
32829794	1	42	theme	hydrogels	254:262	arg1	evaluation					202:211	the comprehensive physico-mechanical evaluation	165:211	the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach	165:393	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	5	43	theme	technique	1123:1131	arg1	combination					1076:1086	combination	1076:1086	combination of an experimental and a numerical technique	1076:1131	Finite element analysis simulation was employed to further advance the calculation accuracy of the nanoindentation tests, concluding that combination of an experimental and a numerical technique may constitute a useful method to characterize the mechanical behavior of composite hydrogel films for use in wound healing applications.
32829794	5	44	theme	element	945:951	arg1	simulation					962:971	Finite element analysis simulation	938:971	Finite element analysis simulation	938:971	Finite element analysis simulation was employed to further advance the calculation accuracy of the nanoindentation tests, concluding that combination of an experimental and a numerical technique may constitute a useful method to characterize the mechanical behavior of composite hydrogel films for use in wound healing applications.
32829794	4	45	theme	wound	917:921	arg1	healing					923:929	an in vitro wound healing	905:929	an in vitro wound healing assay	905:935	All hydrogel compositions showed good biocompatibility on human dermal fibroblasts, stimulating cell growth as confirmed by an in vitro wound healing assay.
32829794	2	46	theme	moist	475:479	arg1	conditions					481:490	moist conditions	475:490	moist conditions	475:490	The 3D printable carbohydrate inks demonstrated good swelling properties under moist conditions and adequate antimicrobial and antibiofilm efficacy against both Gram positive and negative bacteria.
32829794	1	47	with	hydrogels	254:262	arg1	components					279:288	bioactive components	269:288	bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil)	269:344	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	1	48	dep	components	279:288	arg1	oil					341:343	eucalyptus essential oil	320:343	eucalyptus essential oil	320:343	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	1	48	dep	components	279:288	arg1	honey					298:302	Manuka honey	291:302	Manuka honey	291:302	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	1	48	dep	components	279:288	arg1	gel					315:317	aloe vera gel	305:317	aloe vera gel	305:317	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	3	49	theme	3D	682:683	arg1	hydrogels					695:703	the 3D printable hydrogels	678:703	the 3D printable hydrogels	678:703	The effect of the bioactive compounds on the viscosity and mechanical properties of the 3D printable hydrogels was assessed with rheological, nanoindentation and shear test measurements.
32829794	5	50	theme	analysis	953:960	arg1	simulation					962:971	Finite element analysis simulation	938:971	Finite element analysis simulation	938:971	Finite element analysis simulation was employed to further advance the calculation accuracy of the nanoindentation tests, concluding that combination of an experimental and a numerical technique may constitute a useful method to characterize the mechanical behavior of composite hydrogel films for use in wound healing applications.
32829794	1	51	theme	bioactive	269:277	arg1	components					279:288	bioactive components	269:288	bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil)	269:344	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	0	52	theme	3D	61:62	arg1	inks					99:102	3D printable alginate-methylcellulose inks	61:102	3D printable alginate-methylcellulose inks	61:102	Physico-mechanical and finite element analysis evaluation of 3D printable alginate-methylcellulose inks for wound healing applications.
32829794	2	53	theme	negative	575:582	arg1	bacteria					584:591	both Gram positive and negative bacteria	552:591	both Gram positive and negative bacteria	552:591	The 3D printable carbohydrate inks demonstrated good swelling properties under moist conditions and adequate antimicrobial and antibiofilm efficacy against both Gram positive and negative bacteria.
32829794	4	54	theme	good	814:817	arg1	biocompatibility					819:834	good biocompatibility	814:834	good biocompatibility on human dermal fibroblasts, stimulating cell growth	814:887	All hydrogel compositions showed good biocompatibility on human dermal fibroblasts, stimulating cell growth as confirmed by an in vitro wound healing assay.
32829794	3	55	theme	rheological	723:733	arg1	nanoindentation					736:750	nanoindentation	736:750	nanoindentation	736:750	The effect of the bioactive compounds on the viscosity and mechanical properties of the 3D printable hydrogels was assessed with rheological, nanoindentation and shear test measurements.
32829794	3	55	theme	rheological	723:733	arg1	test					762:765	shear test	756:765	shear test	756:765	The effect of the bioactive compounds on the viscosity and mechanical properties of the 3D printable hydrogels was assessed with rheological, nanoindentation and shear test measurements.
32829794	3	55	theme	rheological	723:733	arg1	measurements					767:778	rheological, nanoindentation and shear test measurements	723:778	measurements	767:778	The effect of the bioactive compounds on the viscosity and mechanical properties of the 3D printable hydrogels was assessed with rheological, nanoindentation and shear test measurements.
32829794	4	56	theme	healing	923:929	arg1	assay					931:935	an in vitro wound healing assay	905:935	an in vitro wound healing assay	905:935	All hydrogel compositions showed good biocompatibility on human dermal fibroblasts, stimulating cell growth as confirmed by an in vitro wound healing assay.
32829794	1	57	theme	present	140:146	arg1	study					148:152	The present study	136:152	The present study	136:152	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	1	58	theme	combined	354:361	arg1	approach					386:393	a combined experimental-numerical approach	352:393	a combined experimental-numerical approach	352:393	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	5	59	theme	tests	1053:1057	arg1	accuracy					1021:1028	the calculation accuracy	1005:1028	the calculation accuracy of the nanoindentation tests	1005:1057	Finite element analysis simulation was employed to further advance the calculation accuracy of the nanoindentation tests, concluding that combination of an experimental and a numerical technique may constitute a useful method to characterize the mechanical behavior of composite hydrogel films for use in wound healing applications.
32829794	4	60	from	biocompatibility	819:834	arg1	fibroblasts					852:862	human dermal fibroblasts	839:862	human dermal fibroblasts	839:862	All hydrogel compositions showed good biocompatibility on human dermal fibroblasts, stimulating cell growth as confirmed by an in vitro wound healing assay.
32829794	5	61	theme	experimental	1094:1105	arg1	combination					1076:1086	combination	1076:1086	combination of an experimental and a numerical technique	1076:1131	Finite element analysis simulation was employed to further advance the calculation accuracy of the nanoindentation tests, concluding that combination of an experimental and a numerical technique may constitute a useful method to characterize the mechanical behavior of composite hydrogel films for use in wound healing applications.
32829794	4	62	theme	cell	877:880	arg1	growth					882:887	cell growth	877:887	cell growth	877:887	All hydrogel compositions showed good biocompatibility on human dermal fibroblasts, stimulating cell growth as confirmed by an in vitro wound healing assay.
32829794	3	63	dep	viscosity	639:647	arg1	the					635:637	the	635:637	the	635:637	The effect of the bioactive compounds on the viscosity and mechanical properties of the 3D printable hydrogels was assessed with rheological, nanoindentation and shear test measurements.
32829794	5	64	theme	useful	1150:1155	arg1	method					1157:1162	a useful method	1148:1162	a useful method to characterize the mechanical behavior of composite hydrogel films for use in wound healing applications	1148:1268	Finite element analysis simulation was employed to further advance the calculation accuracy of the nanoindentation tests, concluding that combination of an experimental and a numerical technique may constitute a useful method to characterize the mechanical behavior of composite hydrogel films for use in wound healing applications.
32829794	0	65	theme	alginate-methylcellulose	74:97	arg1	inks					99:102	3D printable alginate-methylcellulose inks	61:102	3D printable alginate-methylcellulose inks	61:102	Physico-mechanical and finite element analysis evaluation of 3D printable alginate-methylcellulose inks for wound healing applications.
32829794	3	66	theme	shear	756:760	arg1	test					762:765	shear test	756:765	shear test	756:765	The effect of the bioactive compounds on the viscosity and mechanical properties of the 3D printable hydrogels was assessed with rheological, nanoindentation and shear test measurements.
32829794	3	66	theme	shear	756:760	arg1	measurements					767:778	rheological, nanoindentation and shear test measurements	723:778	measurements	767:778	The effect of the bioactive compounds on the viscosity and mechanical properties of the 3D printable hydrogels was assessed with rheological, nanoindentation and shear test measurements.
32829794	3	67	theme	printable	685:693	arg1	hydrogels					695:703	the 3D printable hydrogels	678:703	the 3D printable hydrogels	678:703	The effect of the bioactive compounds on the viscosity and mechanical properties of the 3D printable hydrogels was assessed with rheological, nanoindentation and shear test measurements.
32829794	2	68	theme	adequate	496:503	arg1	antimicrobial					505:517	adequate antimicrobial	496:517	adequate antimicrobial	496:517	The 3D printable carbohydrate inks demonstrated good swelling properties under moist conditions and adequate antimicrobial and antibiofilm efficacy against both Gram positive and negative bacteria.
32829794	1	69	theme	Manuka	291:296	arg1	oil					341:343	eucalyptus essential oil	320:343	eucalyptus essential oil	320:343	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	1	69	theme	Manuka	291:296	arg1	honey					298:302	Manuka honey	291:302	Manuka honey	291:302	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	1	69	theme	Manuka	291:296	arg1	gel					315:317	aloe vera gel	305:317	aloe vera gel	305:317	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	0	70	theme	printable	64:72	arg1	inks					99:102	3D printable alginate-methylcellulose inks	61:102	3D printable alginate-methylcellulose inks	61:102	Physico-mechanical and finite element analysis evaluation of 3D printable alginate-methylcellulose inks for wound healing applications.
32829794	1	71	theme	experimental-numerical	363:384	arg1	approach					386:393	a combined experimental-numerical approach	352:393	a combined experimental-numerical approach	352:393	The present study reports on the comprehensive physico-mechanical evaluation of 3D printable alginate-methylcellulose hydrogels with bioactive components (Manuka honey, aloe vera gel, eucalyptus essential oil) using a combined experimental-numerical approach.
32829794	0	72	dep	Physico-mechanical	0:17	arg1	evaluation					47:56	evaluation	47:56	evaluation	47:56	Physico-mechanical and finite element analysis evaluation of 3D printable alginate-methylcellulose inks for wound healing applications.
32829794	2	73	theme	carbohydrate	413:424	arg1	inks					426:429	The 3D printable carbohydrate inks	396:429	The 3D printable carbohydrate inks	396:429	The 3D printable carbohydrate inks demonstrated good swelling properties under moist conditions and adequate antimicrobial and antibiofilm efficacy against both Gram positive and negative bacteria.
34168434	4	0	theme	herb	284:287	arg1	polysaccharides					293:307	polysaccharides	293:307	polysaccharides that have significant anti-diabetic effects	293:351	The main bioactive components of this herb are polysaccharides that have significant anti-diabetic effects.
34168434	4	0	theme	herb	284:287	arg1	components					265:274	The main bioactive components	246:274	The main bioactive components of this herb	246:287	The main bioactive components of this herb are polysaccharides that have significant anti-diabetic effects.
34168434	19	1	theme	cells	1829:1833	arg1	infiltration					1800:1811	infiltration	1800:1811	infiltration of inflammatory cells into the pancreas	1800:1851	The high dose (140mg/kg) of ATMP reduced infiltration of inflammatory cells into the pancreas and restored the structure of islet β-cells in the diabetic mice.
34168434	6	2	theme	mouse	476:480	arg1	model					482:486	a mouse model	474:486	a mouse model of type 1 diabetes mellitus (T1DM)	474:521	polysaccharide (ATMP) on a mouse model of type 1 diabetes mellitus (T1DM).
34168434	13	3	theme	PPARγ	1149:1153	arg1	mRNA					1156:1159	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA	1103:1159	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA	1103:1159	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA and protein levels in the spleen were measured by quantitative real time PCR and Western blotting.
34168434	18	4	theme	tissue	1744:1749	arg1	damage					1751:1756	pancreatic and splenic tissue damage	1721:1756	pancreatic and splenic tissue damage	1721:1756	T1DM was associated with pancreatic and splenic tissue damage.
34168434	17	5	theme	serum	1563:1567	arg1	levels					1577:1582	serum insulin levels	1563:1582	serum insulin levels	1563:1582	In contrast, serum insulin levels, glucose tolerance and body weight improved considerably in the high and medium-dose ATMP and metformin groups.
34168434	16	6	dep	RESULTS	1418:1424	arg1	RESULTS					1418:1424	RESULTS ATMP and metformin	1418:1443	RESULTS ATMP and metformin	1418:1443	RESULTS ATMP and metformin significantly decreased fasting blood glucose, and the food and water intake after 6 weeks of treatment.
34168434	16	6	dep	RESULTS	1418:1424	arg1	metformin					1435:1443	metformin	1435:1443	metformin	1435:1443	RESULTS ATMP and metformin significantly decreased fasting blood glucose, and the food and water intake after 6 weeks of treatment.
34168434	16	6	dep	RESULTS	1418:1424	arg1	ATMP					1426:1429	ATMP	1426:1429	ATMP	1426:1429	RESULTS ATMP and metformin significantly decreased fasting blood glucose, and the food and water intake after 6 weeks of treatment.
34168434	5	7	theme	Merr	443:446	arg1	effect					413:418	the immunoregulatory effect	392:418	the immunoregulatory effect of A. trifoliatus (L.) Merr	392:446	The aim of this study was to evaluate the immunoregulatory effect of A. trifoliatus (L.) Merr.
34168434	13	8	theme	proliferator-activated	1114:1135	arg1	mRNA					1156:1159	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA	1103:1159	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA	1103:1159	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA and protein levels in the spleen were measured by quantitative real time PCR and Western blotting.
34168434	20	9	theme	140mg/kg	1952:1959	arg1	ATMP					1961:1964	140mg/kg ATMP	1952:1964	140mg/kg ATMP	1952:1964	Consistent with this, 35, 70 and 140mg/kg ATMP increased IL-10 levels and decreased that of IFN-γ, thereby restoring the CD4+/CD8+ and Th1/Th2 cytokine ratio.
34168434	4	10	theme	anti-diabetic	331:343	arg1	effects					345:351	significant anti-diabetic effects	319:351	significant anti-diabetic effects	319:351	The main bioactive components of this herb are polysaccharides that have significant anti-diabetic effects.
34168434	22	11	theme	immune	2237:2242	arg1	balance					2244:2250	the immune balance	2233:2250	the immune balance in the spleen	2233:2264	CONCLUSION ATMP exerts a hypoglycemic effect in diabetic mice by restoring the immune balance in the spleen.
34168434	5	12	theme	trifoliatus	426:436	arg1	Merr					443:446	A. trifoliatus (L.) Merr	423:446	A. trifoliatus (L.) Merr	423:446	The aim of this study was to evaluate the immunoregulatory effect of A. trifoliatus (L.) Merr.
34168434	6	13	theme	mellitus	507:514	arg1	model					482:486	a mouse model	474:486	a mouse model of type 1 diabetes mellitus (T1DM)	474:521	polysaccharide (ATMP) on a mouse model of type 1 diabetes mellitus (T1DM).
34168434	10	14	theme	glucose	899:905	arg1	levels					907:912	fasting glucose levels	891:912	fasting glucose levels	891:912	The body weight of the mice, food and water intake and fasting glucose levels were monitored throughout the 6-week regimen.
34168434	12	15	theme	Oral	1039:1042	arg1	tolerance					1052:1060	Oral glucose tolerance	1039:1060	Oral glucose tolerance	1039:1060	Oral glucose tolerance was evaluated with the appropriate test.
34168434	10	16	theme	6-week	944:949	arg1	regimen					951:957	the 6-week regimen	940:957	the 6-week regimen	940:957	The body weight of the mice, food and water intake and fasting glucose levels were monitored throughout the 6-week regimen.
34168434	13	17	theme	time	1229:1232	arg1	PCR					1234:1236	quantitative real time PCR	1211:1236	quantitative real time PCR	1211:1236	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA and protein levels in the spleen were measured by quantitative real time PCR and Western blotting.
34168434	7	18	theme	ATMP	590:593	arg1	composition					551:561	monosaccharide composition	536:561	monosaccharide composition	536:561	METHODS The monosaccharide composition and mean molecular mass of ATMP were determined by HPLC and HPGPC.
34168434	7	18	theme	ATMP	590:593	arg1	mass					582:585	mean molecular mass	567:585	mean molecular mass	567:585	METHODS The monosaccharide composition and mean molecular mass of ATMP were determined by HPLC and HPGPC.
34168434	7	18	theme	ATMP	590:593	arg1	METHODS					524:530	METHODS	524:530	METHODS The monosaccharide composition and mean molecular mass of ATMP	524:593	METHODS The monosaccharide composition and mean molecular mass of ATMP were determined by HPLC and HPGPC.
34168434	15	19	theme	spleen	1358:1363	arg1	tissues					1365:1371	spleen tissues	1358:1371	spleen tissues	1358:1371	The CD4+ and CD8+T cells in spleen tissues were detected by immunohistochemistry (IHC).
34168434	5	20	theme	study	370:374	arg1	aim					358:360	The aim	354:360	The aim of this study	354:374	The aim of this study was to evaluate the immunoregulatory effect of A. trifoliatus (L.) Merr.
34168434	8	21	theme	140mg/kg	681:688	arg1	ATMP					690:693	140mg/kg ATMP	681:693	140mg/kg ATMP	681:693	T1DM was induced in mice using STZ, and 35, 70 and 140mg/kg ATMP was administered daily via the intragastric route for six weeks.
34168434	12	22	theme	appropriate	1085:1095	arg1	test					1097:1100	the appropriate test	1081:1100	the appropriate test	1081:1100	Oral glucose tolerance was evaluated with the appropriate test.
34168434	7	23	theme	mean	567:570	arg1	mass					582:585	mean molecular mass	567:585	mean molecular mass	567:585	METHODS The monosaccharide composition and mean molecular mass of ATMP were determined by HPLC and HPGPC.
34168434	7	23	theme	mean	567:570	arg1	METHODS					524:530	METHODS	524:530	METHODS The monosaccharide composition and mean molecular mass of ATMP	524:593	METHODS The monosaccharide composition and mean molecular mass of ATMP were determined by HPLC and HPGPC.
34168434	16	24	theme	water	1509:1513	arg1	intake					1515:1520	the food and water intake	1496:1520	intake	1515:1520	RESULTS ATMP and metformin significantly decreased fasting blood glucose, and the food and water intake after 6 weeks of treatment.
34168434	4	25	contain	have	314:317	arg2	effects					345:351	significant anti-diabetic effects	319:351	significant anti-diabetic effects	319:351	The main bioactive components of this herb are polysaccharides that have significant anti-diabetic effects.
34168434	4	25	contain	have	314:317	arg1	polysaccharides					293:307	polysaccharides	293:307	polysaccharides that have significant anti-diabetic effects	293:351	The main bioactive components of this herb are polysaccharides that have significant anti-diabetic effects.
34168434	4	25	contain	have	314:317	arg1	components					265:274	The main bioactive components	246:274	The main bioactive components of this herb	246:287	The main bioactive components of this herb are polysaccharides that have significant anti-diabetic effects.
34168434	13	26	theme	quantitative	1211:1222	arg1	PCR					1234:1236	quantitative real time PCR	1211:1236	quantitative real time PCR	1211:1236	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA and protein levels in the spleen were measured by quantitative real time PCR and Western blotting.
34168434	12	27	theme	glucose	1044:1050	arg1	tolerance					1052:1060	Oral glucose tolerance	1039:1060	Oral glucose tolerance	1039:1060	Oral glucose tolerance was evaluated with the appropriate test.
34168434	4	28	theme	main	250:253	arg1	polysaccharides					293:307	polysaccharides	293:307	polysaccharides that have significant anti-diabetic effects	293:351	The main bioactive components of this herb are polysaccharides that have significant anti-diabetic effects.
34168434	4	28	theme	main	250:253	arg1	components					265:274	The main bioactive components	246:274	The main bioactive components of this herb	246:287	The main bioactive components of this herb are polysaccharides that have significant anti-diabetic effects.
34168434	3	29	theme	medicinal	144:152	arg1	plant					154:158	a medicinal plant	142:158	a medicinal plant found in Southeast Asia	142:182	is a medicinal plant found in Southeast Asia, and its young leaves and shoots are consumed as a vegetable.
34168434	19	30	theme	islet	1883:1887	arg1	β-cells					1889:1895	islet β-cells	1883:1895	islet β-cells	1883:1895	The high dose (140mg/kg) of ATMP reduced infiltration of inflammatory cells into the pancreas and restored the structure of islet β-cells in the diabetic mice.
34168434	0	31	theme	Immunoregulatory	0:15	arg1	Effect					17:22	Immunoregulatory Effect	0:22	Immunoregulatory Effect of Acanthopanax trifoliatus (L.)	0:55	Immunoregulatory Effect of Acanthopanax trifoliatus (L.) Merr.
34168434	20	32	theme	cytokine	2062:2069	arg1	ratio					2071:2075	the CD4+/CD8+ and Th1/Th2 cytokine ratio	2036:2075	ratio	2071:2075	Consistent with this, 35, 70 and 140mg/kg ATMP increased IL-10 levels and decreased that of IFN-γ, thereby restoring the CD4+/CD8+ and Th1/Th2 cytokine ratio.
34168434	19	33	theme	high	1763:1766	arg1	dose					1768:1771	The high dose	1759:1771	The high dose (140mg/kg) of ATMP	1759:1790	The high dose (140mg/kg) of ATMP reduced infiltration of inflammatory cells into the pancreas and restored the structure of islet β-cells in the diabetic mice.
34168434	19	33	theme	high	1763:1766	arg1	140mg/kg					1774:1781	140mg/kg	1774:1781	140mg/kg	1774:1781	The high dose (140mg/kg) of ATMP reduced infiltration of inflammatory cells into the pancreas and restored the structure of islet β-cells in the diabetic mice.
34168434	19	34	theme	diabetic	1904:1911	arg1	mice					1913:1916	the diabetic mice	1900:1916	the diabetic mice	1900:1916	The high dose (140mg/kg) of ATMP reduced infiltration of inflammatory cells into the pancreas and restored the structure of islet β-cells in the diabetic mice.
34168434	17	35	theme	ATMP	1669:1672	arg1	groups					1688:1693	the high and medium-dose ATMP and metformin groups	1644:1693	the high and medium-dose ATMP and metformin groups	1644:1693	In contrast, serum insulin levels, glucose tolerance and body weight improved considerably in the high and medium-dose ATMP and metformin groups.
34168434	0	36	dep	Merr	57:60	arg1	Effect					17:22	Immunoregulatory Effect	0:22	Immunoregulatory Effect of Acanthopanax trifoliatus (L.)	0:55	Immunoregulatory Effect of Acanthopanax trifoliatus (L.) Merr.
34168434	22	37	from	balance	2244:2250	arg1	spleen					2259:2264	the spleen	2255:2264	the spleen	2255:2264	CONCLUSION ATMP exerts a hypoglycemic effect in diabetic mice by restoring the immune balance in the spleen.
34168434	19	38	theme	ATMP	1787:1790	arg1	dose					1768:1771	The high dose	1759:1771	The high dose (140mg/kg) of ATMP	1759:1790	The high dose (140mg/kg) of ATMP reduced infiltration of inflammatory cells into the pancreas and restored the structure of islet β-cells in the diabetic mice.
34168434	19	38	theme	ATMP	1787:1790	arg1	140mg/kg					1774:1781	140mg/kg	1774:1781	140mg/kg	1774:1781	The high dose (140mg/kg) of ATMP reduced infiltration of inflammatory cells into the pancreas and restored the structure of islet β-cells in the diabetic mice.
34168434	9	39	theme	Untreated	760:768	arg1	groups					809:814	Untreated and metformin-treated positive control groups	760:814	Untreated and metformin-treated positive control groups	760:814	Untreated and metformin-treated positive control groups were also included.
34168434	5	40	dep	trifoliatus	426:436	arg1	L.					439:440	L.	439:440	L.	439:440	The aim of this study was to evaluate the immunoregulatory effect of A. trifoliatus (L.) Merr.
34168434	0	41	theme	trifoliatus	40:50	arg1	Effect					17:22	Immunoregulatory Effect	0:22	Immunoregulatory Effect of Acanthopanax trifoliatus (L.)	0:55	Immunoregulatory Effect of Acanthopanax trifoliatus (L.) Merr.
34168434	17	42	theme	metformin	1678:1686	arg1	groups					1688:1693	the high and medium-dose ATMP and metformin groups	1644:1693	the high and medium-dose ATMP and metformin groups	1644:1693	In contrast, serum insulin levels, glucose tolerance and body weight improved considerably in the high and medium-dose ATMP and metformin groups.
34168434	17	43	theme	body	1607:1610	arg1	weight					1612:1617	body weight	1607:1617	body weight	1607:1617	In contrast, serum insulin levels, glucose tolerance and body weight improved considerably in the high and medium-dose ATMP and metformin groups.
34168434	2	44	dep	Acanthopanax	103:114	arg1	trifoliatus					116:126	Acanthopanax trifoliatus	103:126	Acanthopanax trifoliatus (L.) Merr	103:136	BACKGROUND Acanthopanax trifoliatus (L.) Merr.
34168434	15	45	from	cells	1349:1353	arg1	tissues					1365:1371	spleen tissues	1358:1371	spleen tissues	1358:1371	The CD4+ and CD8+T cells in spleen tissues were detected by immunohistochemistry (IHC).
34168434	13	46	from	levels	1173:1178	arg1	spleen					1187:1192	the spleen	1183:1192	the spleen	1183:1192	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA and protein levels in the spleen were measured by quantitative real time PCR and Western blotting.
34168434	22	47	theme	hypoglycemic	2183:2194	arg1	effect					2196:2201	a hypoglycemic effect	2181:2201	a hypoglycemic effect	2181:2201	CONCLUSION ATMP exerts a hypoglycemic effect in diabetic mice by restoring the immune balance in the spleen.
34168434	4	48	theme	significant	319:329	arg1	effects					345:351	significant anti-diabetic effects	319:351	significant anti-diabetic effects	319:351	The main bioactive components of this herb are polysaccharides that have significant anti-diabetic effects.
34168434	19	49	theme	inflammatory	1816:1827	arg1	cells					1829:1833	inflammatory cells	1816:1833	inflammatory cells	1816:1833	The high dose (140mg/kg) of ATMP reduced infiltration of inflammatory cells into the pancreas and restored the structure of islet β-cells in the diabetic mice.
34168434	9	50	theme	control	801:807	arg1	groups					809:814	Untreated and metformin-treated positive control groups	760:814	Untreated and metformin-treated positive control groups	760:814	Untreated and metformin-treated positive control groups were also included.
34168434	18	51	theme	pancreatic	1721:1730	arg1	damage					1751:1756	pancreatic and splenic tissue damage	1721:1756	pancreatic and splenic tissue damage	1721:1756	T1DM was associated with pancreatic and splenic tissue damage.
34168434	7	52	dep	METHODS	524:530	arg1	composition					551:561	monosaccharide composition	536:561	monosaccharide composition	536:561	METHODS The monosaccharide composition and mean molecular mass of ATMP were determined by HPLC and HPGPC.
34168434	7	52	dep	METHODS	524:530	arg1	mass					582:585	mean molecular mass	567:585	mean molecular mass	567:585	METHODS The monosaccharide composition and mean molecular mass of ATMP were determined by HPLC and HPGPC.
34168434	7	52	dep	METHODS	524:530	arg1	METHODS					524:530	METHODS	524:530	METHODS The monosaccharide composition and mean molecular mass of ATMP	524:593	METHODS The monosaccharide composition and mean molecular mass of ATMP were determined by HPLC and HPGPC.
34168434	6	53	theme	type	491:494	arg1	mellitus					507:514	type 1 diabetes mellitus	491:514	type 1 diabetes mellitus (T1DM)	491:521	polysaccharide (ATMP) on a mouse model of type 1 diabetes mellitus (T1DM).
34168434	6	53	theme	type	491:494	arg1	T1DM					517:520	T1DM	517:520	T1DM	517:520	polysaccharide (ATMP) on a mouse model of type 1 diabetes mellitus (T1DM).
34168434	21	54	theme	molecular	2085:2093	arg1	level					2095:2099	the molecular level	2081:2099	the molecular level	2081:2099	At the molecular level, high-dose ATMP up-regulated PPARγ in the splenic cells.
34168434	20	55	with	Consistent	1919:1928	arg1	this					1935:1938	this	1935:1938	this	1935:1938	Consistent with this, 35, 70 and 140mg/kg ATMP increased IL-10 levels and decreased that of IFN-γ, thereby restoring the CD4+/CD8+ and Th1/Th2 cytokine ratio.
34168434	17	56	theme	high	1648:1651	arg1	groups					1688:1693	the high and medium-dose ATMP and metformin groups	1644:1693	the high and medium-dose ATMP and metformin groups	1644:1693	In contrast, serum insulin levels, glucose tolerance and body weight improved considerably in the high and medium-dose ATMP and metformin groups.
34168434	11	57	from	changes	973:979	arg1	spleen					1001:1006	spleen	1001:1006	spleen	1001:1006	Histological changes in the pancreas and spleen were analyzed by H&E staining.
34168434	11	57	from	changes	973:979	arg1	pancreas					988:995	pancreas	988:995	pancreas	988:995	Histological changes in the pancreas and spleen were analyzed by H&E staining.
34168434	9	58	theme	metformin-treated	774:790	arg1	groups					809:814	Untreated and metformin-treated positive control groups	760:814	Untreated and metformin-treated positive control groups	760:814	Untreated and metformin-treated positive control groups were also included.
34168434	17	59	theme	glucose	1585:1591	arg1	tolerance					1593:1601	glucose tolerance	1585:1601	glucose tolerance	1585:1601	In contrast, serum insulin levels, glucose tolerance and body weight improved considerably in the high and medium-dose ATMP and metformin groups.
34168434	18	60	theme	splenic	1736:1742	arg1	damage					1751:1756	pancreatic and splenic tissue damage	1721:1756	pancreatic and splenic tissue damage	1721:1756	T1DM was associated with pancreatic and splenic tissue damage.
34168434	17	61	theme	insulin	1569:1575	arg1	levels					1577:1582	serum insulin levels	1563:1582	serum insulin levels	1563:1582	In contrast, serum insulin levels, glucose tolerance and body weight improved considerably in the high and medium-dose ATMP and metformin groups.
34168434	13	62	theme	Peroxisome	1103:1112	arg1	mRNA					1156:1159	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA	1103:1159	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA	1103:1159	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA and protein levels in the spleen were measured by quantitative real time PCR and Western blotting.
34168434	11	63	theme	Histological	960:971	arg1	changes					973:979	Histological changes	960:979	Histological changes in the pancreas and spleen	960:1006	Histological changes in the pancreas and spleen were analyzed by H&E staining.
34168434	13	64	theme	receptor	1137:1144	arg1	mRNA					1156:1159	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA	1103:1159	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA	1103:1159	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA and protein levels in the spleen were measured by quantitative real time PCR and Western blotting.
34168434	20	65	theme	IL-10	1976:1980	arg1	levels					1982:1987	IL-10 levels	1976:1987	IL-10 levels	1976:1987	Consistent with this, 35, 70 and 140mg/kg ATMP increased IL-10 levels and decreased that of IFN-γ, thereby restoring the CD4+/CD8+ and Th1/Th2 cytokine ratio.
34168434	3	66	dep	young	193:197	arg1	leaves					199:204	leaves	199:204	leaves	199:204	is a medicinal plant found in Southeast Asia, and its young leaves and shoots are consumed as a vegetable.
34168434	21	67	theme	splenic	2143:2149	arg1	cells					2151:2155	the splenic cells	2139:2155	the splenic cells	2139:2155	At the molecular level, high-dose ATMP up-regulated PPARγ in the splenic cells.
34168434	6	68	theme	diabetes	498:505	arg1	mellitus					507:514	type 1 diabetes mellitus	491:514	type 1 diabetes mellitus (T1DM)	491:521	polysaccharide (ATMP) on a mouse model of type 1 diabetes mellitus (T1DM).
34168434	6	68	theme	diabetes	498:505	arg1	T1DM					517:520	T1DM	517:520	T1DM	517:520	polysaccharide (ATMP) on a mouse model of type 1 diabetes mellitus (T1DM).
34168434	10	69	theme	fasting	891:897	arg1	levels					907:912	fasting glucose levels	891:912	fasting glucose levels	891:912	The body weight of the mice, food and water intake and fasting glucose levels were monitored throughout the 6-week regimen.
34168434	7	70	dep	composition	551:561	arg1	The					532:534	The	532:534	The	532:534	METHODS The monosaccharide composition and mean molecular mass of ATMP were determined by HPLC and HPGPC.
34168434	16	71	theme	treatment	1539:1547	arg1	weeks					1530:1534	6 weeks	1528:1534	6 weeks of treatment	1528:1547	RESULTS ATMP and metformin significantly decreased fasting blood glucose, and the food and water intake after 6 weeks of treatment.
34168434	14	72	theme	insulin	1277:1283	arg1	levels					1285:1290	IL-10, IFN-γ and insulin levels	1260:1290	IL-10, IFN-γ and insulin levels in the sera	1260:1302	IL-10, IFN-γ and insulin levels in the sera were determined by ELISA.
34168434	16	73	theme	blood	1477:1481	arg1	glucose					1483:1489	blood glucose	1477:1489	blood glucose	1477:1489	RESULTS ATMP and metformin significantly decreased fasting blood glucose, and the food and water intake after 6 weeks of treatment.
34168434	13	74	theme	Western	1242:1248	arg1	blotting					1250:1257	Western blotting	1242:1257	Western blotting	1242:1257	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA and protein levels in the spleen were measured by quantitative real time PCR and Western blotting.
34168434	8	75	theme	intragastric	726:737	arg1	route					739:743	the intragastric route	722:743	the intragastric route	722:743	T1DM was induced in mice using STZ, and 35, 70 and 140mg/kg ATMP was administered daily via the intragastric route for six weeks.
34168434	14	76	theme	IFN-γ	1267:1271	arg1	levels					1285:1290	IL-10, IFN-γ and insulin levels	1260:1290	IL-10, IFN-γ and insulin levels in the sera	1260:1302	IL-10, IFN-γ and insulin levels in the sera were determined by ELISA.
34168434	15	77	theme	CD8+T	1343:1347	arg1	cells					1349:1353	The CD4+ and CD8+T cells	1330:1353	The CD4+ and CD8+T cells in spleen tissues	1330:1371	The CD4+ and CD8+T cells in spleen tissues were detected by immunohistochemistry (IHC).
34168434	19	78	theme	β-cells	1889:1895	arg1	structure					1870:1878	the structure	1866:1878	the structure of islet β-cells in the diabetic mice	1866:1916	The high dose (140mg/kg) of ATMP reduced infiltration of inflammatory cells into the pancreas and restored the structure of islet β-cells in the diabetic mice.
34168434	7	79	theme	molecular	572:580	arg1	mass					582:585	mean molecular mass	567:585	mean molecular mass	567:585	METHODS The monosaccharide composition and mean molecular mass of ATMP were determined by HPLC and HPGPC.
34168434	7	79	theme	molecular	572:580	arg1	METHODS					524:530	METHODS	524:530	METHODS The monosaccharide composition and mean molecular mass of ATMP	524:593	METHODS The monosaccharide composition and mean molecular mass of ATMP were determined by HPLC and HPGPC.
34168434	14	80	theme	IL-10	1260:1264	arg1	levels					1285:1290	IL-10, IFN-γ and insulin levels	1260:1290	IL-10, IFN-γ and insulin levels in the sera	1260:1302	IL-10, IFN-γ and insulin levels in the sera were determined by ELISA.
34168434	13	81	theme	real	1224:1227	arg1	PCR					1234:1236	quantitative real time PCR	1211:1236	quantitative real time PCR	1211:1236	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA and protein levels in the spleen were measured by quantitative real time PCR and Western blotting.
34168434	16	82	theme	food	1500:1503	arg1	intake					1515:1520	the food and water intake	1496:1520	intake	1515:1520	RESULTS ATMP and metformin significantly decreased fasting blood glucose, and the food and water intake after 6 weeks of treatment.
34168434	15	83	theme	CD4+	1334:1337	arg1	cells					1349:1353	The CD4+ and CD8+T cells	1330:1353	The CD4+ and CD8+T cells in spleen tissues	1330:1371	The CD4+ and CD8+T cells in spleen tissues were detected by immunohistochemistry (IHC).
34168434	7	84	theme	monosaccharide	536:549	arg1	composition					551:561	monosaccharide composition	536:561	monosaccharide composition	536:561	METHODS The monosaccharide composition and mean molecular mass of ATMP were determined by HPLC and HPGPC.
34168434	7	84	theme	monosaccharide	536:549	arg1	METHODS					524:530	METHODS	524:530	METHODS The monosaccharide composition and mean molecular mass of ATMP	524:593	METHODS The monosaccharide composition and mean molecular mass of ATMP were determined by HPLC and HPGPC.
34168434	1	85	theme	T1DM	81:84	arg1	Mice					86:89	T1DM Mice	81:89	T1DM Mice	81:89	Polysaccharide on T1DM Mice.
34168434	4	86	theme	bioactive	255:263	arg1	polysaccharides					293:307	polysaccharides	293:307	polysaccharides that have significant anti-diabetic effects	293:351	The main bioactive components of this herb are polysaccharides that have significant anti-diabetic effects.
34168434	4	86	theme	bioactive	255:263	arg1	components					265:274	The main bioactive components	246:274	The main bioactive components of this herb	246:287	The main bioactive components of this herb are polysaccharides that have significant anti-diabetic effects.
34168434	2	87	dep	Merr	133:136	arg1	BACKGROUND					92:101	BACKGROUND	92:101	BACKGROUND	92:101	BACKGROUND Acanthopanax trifoliatus (L.) Merr.
34168434	0	88	theme	Acanthopanax	27:38	arg1	L.					53:54	L.	53:54	L.	53:54	Immunoregulatory Effect of Acanthopanax trifoliatus (L.) Merr.
34168434	0	88	theme	Acanthopanax	27:38	arg1	trifoliatus					40:50	Acanthopanax trifoliatus	27:50	Acanthopanax trifoliatus (L.)	27:55	Immunoregulatory Effect of Acanthopanax trifoliatus (L.) Merr.
34168434	20	89	theme	Th1/Th2	2054:2060	arg1	ratio					2071:2075	the CD4+/CD8+ and Th1/Th2 cytokine ratio	2036:2075	ratio	2071:2075	Consistent with this, 35, 70 and 140mg/kg ATMP increased IL-10 levels and decreased that of IFN-γ, thereby restoring the CD4+/CD8+ and Th1/Th2 cytokine ratio.
34168434	17	90	theme	medium-dose	1657:1667	arg1	groups					1688:1693	the high and medium-dose ATMP and metformin groups	1644:1693	the high and medium-dose ATMP and metformin groups	1644:1693	In contrast, serum insulin levels, glucose tolerance and body weight improved considerably in the high and medium-dose ATMP and metformin groups.
34168434	10	91	theme	body	840:843	arg1	weight					845:850	The body weight	836:850	The body weight of the mice	836:862	The body weight of the mice, food and water intake and fasting glucose levels were monitored throughout the 6-week regimen.
34168434	3	92	located	found	160:164	arg1	Asia					179:182	Asia	179:182	Asia	179:182	is a medicinal plant found in Southeast Asia, and its young leaves and shoots are consumed as a vegetable.
34168434	3	92	located	found	160:164	arg2	plant					154:158	a medicinal plant	142:158	a medicinal plant found in Southeast Asia	142:182	is a medicinal plant found in Southeast Asia, and its young leaves and shoots are consumed as a vegetable.
34168434	22	93	theme	diabetic	2206:2213	arg1	mice					2215:2218	diabetic mice	2206:2218	diabetic mice	2206:2218	CONCLUSION ATMP exerts a hypoglycemic effect in diabetic mice by restoring the immune balance in the spleen.
34168434	10	94	dep	food	865:868	arg1	intake					880:885	intake	880:885	intake	880:885	The body weight of the mice, food and water intake and fasting glucose levels were monitored throughout the 6-week regimen.
34168434	21	95	theme	high-dose	2102:2110	arg1	ATMP					2112:2115	high-dose ATMP	2102:2115	high-dose ATMP	2102:2115	At the molecular level, high-dose ATMP up-regulated PPARγ in the splenic cells.
34168434	10	96	theme	mice	859:862	arg1	levels					907:912	fasting glucose levels	891:912	fasting glucose levels	891:912	The body weight of the mice, food and water intake and fasting glucose levels were monitored throughout the 6-week regimen.
34168434	10	96	theme	mice	859:862	arg1	water					874:878	water	874:878	water	874:878	The body weight of the mice, food and water intake and fasting glucose levels were monitored throughout the 6-week regimen.
34168434	10	96	theme	mice	859:862	arg1	food					865:868	food	865:868	food	865:868	The body weight of the mice, food and water intake and fasting glucose levels were monitored throughout the 6-week regimen.
34168434	10	96	theme	mice	859:862	arg1	weight					845:850	The body weight	836:850	The body weight of the mice	836:862	The body weight of the mice, food and water intake and fasting glucose levels were monitored throughout the 6-week regimen.
34168434	6	97	dep	polysaccharide	449:462	arg1	model					482:486	a mouse model	474:486	a mouse model of type 1 diabetes mellitus (T1DM)	474:521	polysaccharide (ATMP) on a mouse model of type 1 diabetes mellitus (T1DM).
34168434	9	98	theme	positive	792:799	arg1	groups					809:814	Untreated and metformin-treated positive control groups	760:814	Untreated and metformin-treated positive control groups	760:814	Untreated and metformin-treated positive control groups were also included.
34168434	13	99	theme	mRNA	1156:1159	arg1	levels					1173:1178	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA and protein levels	1103:1178	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA and protein levels in the spleen	1103:1192	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA and protein levels in the spleen were measured by quantitative real time PCR and Western blotting.
34168434	5	100	theme	immunoregulatory	396:411	arg1	effect					413:418	the immunoregulatory effect	392:418	the immunoregulatory effect of A. trifoliatus (L.) Merr	392:446	The aim of this study was to evaluate the immunoregulatory effect of A. trifoliatus (L.) Merr.
34168434	13	101	theme	protein	1165:1171	arg1	levels					1173:1178	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA and protein levels	1103:1178	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA and protein levels in the spleen	1103:1192	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA and protein levels in the spleen were measured by quantitative real time PCR and Western blotting.
34168434	19	102	from	structure	1870:1878	arg1	mice					1913:1916	the diabetic mice	1900:1916	the diabetic mice	1900:1916	The high dose (140mg/kg) of ATMP reduced infiltration of inflammatory cells into the pancreas and restored the structure of islet β-cells in the diabetic mice.
34168434	11	103	theme	H&E	1025:1027	arg1	staining					1029:1036	H&E staining	1025:1036	H&E staining	1025:1036	Histological changes in the pancreas and spleen were analyzed by H&E staining.
34168434	14	104	from	levels	1285:1290	arg1	sera					1299:1302	the sera	1295:1302	the sera	1295:1302	IL-10, IFN-γ and insulin levels in the sera were determined by ELISA.
34168434	13	105	theme	γ	1146:1146	arg1	mRNA					1156:1159	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA	1103:1159	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA	1103:1159	Peroxisome proliferator-activated receptor γ (PPARγ) mRNA and protein levels in the spleen were measured by quantitative real time PCR and Western blotting.
34168434	1	106	from	Polysaccharide	63:76	arg1	Mice					86:89	T1DM Mice	81:89	T1DM Mice	81:89	Polysaccharide on T1DM Mice.
32501668	8	0	theme	fast	1404:1407	arg1	mixing					1433:1438	The fast and efficient nanoscale mixing	1400:1438	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups	1400:1495	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups is unique, novel, and promising as a functional material platform.
32501668	8	0	theme	fast	1404:1407	arg1	unique					1500:1505	unique	1500:1505	unique	1500:1505	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups is unique, novel, and promising as a functional material platform.
32501668	4	1	theme	ice-templated	896:908	arg1	mixtures					920:927	ice-templated CNF/Helux mixtures	896:927	ice-templated CNF/Helux mixtures	896:927	Non-cross-linked mixtures of CNF/Helux were assembled into composites, such as films by solvent casting and aerogels with densities as low as 4 kg/m3 by lyophilizing ice-templated CNF/Helux mixtures.
32501668	2	2	theme	resulting	372:380	arg1	mixtures					392:399	the resulting composite mixtures	368:399	the resulting composite mixtures of CNFs and Helux	368:417	The polyampholytic character of Helux enabled facile and efficient nanoscale mixing with the CNFs, and the resulting composite mixtures of CNFs and Helux displayed thixotropic behavior and formed physical and reversibly cross-linked gels when left unperturbed for short spans of time.
32501668	3	3	contain	containing	628:637	arg1	hydrogels					618:626	self-supporting solid hydrogels	596:626	self-supporting solid hydrogels containing impressive water contents of 99.6% and a storage modulus of 1.8 kPa	596:705	The gel could be chemically cross-linked into self-supporting solid hydrogels containing impressive water contents of 99.6% and a storage modulus of 1.8 kPa by thermal activation.
32501668	3	3	contain	containing	628:637	arg2	modulus					688:694	a storage modulus	678:694	a storage modulus of 1.8 kPa	678:705	The gel could be chemically cross-linked into self-supporting solid hydrogels containing impressive water contents of 99.6% and a storage modulus of 1.8 kPa by thermal activation.
32501668	3	3	contain	containing	628:637	arg2	contents					656:663	impressive water contents	639:663	impressive water contents of 99.6%	639:672	The gel could be chemically cross-linked into self-supporting solid hydrogels containing impressive water contents of 99.6% and a storage modulus of 1.8 kPa by thermal activation.
32501668	5	4	theme	heat-activated	999:1012	arg1	cross-linking					1014:1026	the heat-activated cross-linking	995:1026	the heat-activated cross-linking	995:1026	The resulting materials exhibited excellent wet stability due to the heat-activated cross-linking and were readily available for postfunctionalization via amidation chemistry using Helux-accessible amines in aqueous conditions.
32501668	3	5	theme	thermal	710:716	arg1	activation					718:727	thermal activation	710:727	thermal activation	710:727	The gel could be chemically cross-linked into self-supporting solid hydrogels containing impressive water contents of 99.6% and a storage modulus of 1.8 kPa by thermal activation.
32501668	5	6	theme	resulting	934:942	arg1	available					1045:1053	available	1045:1053	available	1045:1053	The resulting materials exhibited excellent wet stability due to the heat-activated cross-linking and were readily available for postfunctionalization via amidation chemistry using Helux-accessible amines in aqueous conditions.
32501668	5	6	theme	resulting	934:942	arg1	materials					944:952	The resulting materials	930:952	The resulting materials	930:952	The resulting materials exhibited excellent wet stability due to the heat-activated cross-linking and were readily available for postfunctionalization via amidation chemistry using Helux-accessible amines in aqueous conditions.
32501668	8	7	theme	material	1545:1552	arg1	platform					1554:1561	a functional material platform	1532:1561	a functional material platform	1532:1561	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups is unique, novel, and promising as a functional material platform.
32501668	7	8	link	non-cross-linked	1358:1373	arg1	states					1392:1397	both the non-cross-linked and cross-linked states	1349:1397	states	1392:1397	Additionally, by varying the amount of Helux, the compressive elastic modulus of aerogels was tunable in both the non-cross-linked and cross-linked states.
32501668	4	9	theme	solvent	818:824	arg1	casting					826:832	solvent casting	818:832	solvent casting	818:832	Non-cross-linked mixtures of CNF/Helux were assembled into composites, such as films by solvent casting and aerogels with densities as low as 4 kg/m3 by lyophilizing ice-templated CNF/Helux mixtures.
32501668	7	10	link	cross-linked	1379:1390	arg1	states					1392:1397	both the non-cross-linked and cross-linked states	1349:1397	states	1392:1397	Additionally, by varying the amount of Helux, the compressive elastic modulus of aerogels was tunable in both the non-cross-linked and cross-linked states.
32501668	8	11	theme	nanoscale	1423:1431	arg1	mixing					1433:1438	The fast and efficient nanoscale mixing	1400:1438	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups	1400:1495	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups is unique, novel, and promising as a functional material platform.
32501668	8	11	theme	nanoscale	1423:1431	arg1	unique					1500:1505	unique	1500:1505	unique	1500:1505	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups is unique, novel, and promising as a functional material platform.
32501668	2	12	theme	efficient	322:330	arg1	mixing					342:347	facile and efficient nanoscale mixing	311:347	facile and efficient nanoscale mixing with the CNFs	311:361	The polyampholytic character of Helux enabled facile and efficient nanoscale mixing with the CNFs, and the resulting composite mixtures of CNFs and Helux displayed thixotropic behavior and formed physical and reversibly cross-linked gels when left unperturbed for short spans of time.
32501668	4	13	with	casting	826:832	arg1	densities					852:860	densities	852:860	densities as low as 4 kg/m3 by lyophilizing ice-templated CNF/Helux mixtures	852:927	Non-cross-linked mixtures of CNF/Helux were assembled into composites, such as films by solvent casting and aerogels with densities as low as 4 kg/m3 by lyophilizing ice-templated CNF/Helux mixtures.
32501668	4	14	link	Non-cross-linked	730:745	arg1	mixtures					747:754	Non-cross-linked mixtures	730:754	Non-cross-linked mixtures of CNF/Helux	730:767	Non-cross-linked mixtures of CNF/Helux were assembled into composites, such as films by solvent casting and aerogels with densities as low as 4 kg/m3 by lyophilizing ice-templated CNF/Helux mixtures.
32501668	4	15	theme	CNF/Helux	910:918	arg1	mixtures					920:927	ice-templated CNF/Helux mixtures	896:927	ice-templated CNF/Helux mixtures	896:927	Non-cross-linked mixtures of CNF/Helux were assembled into composites, such as films by solvent casting and aerogels with densities as low as 4 kg/m3 by lyophilizing ice-templated CNF/Helux mixtures.
32501668	2	16	theme	facile	311:316	arg1	mixing					342:347	facile and efficient nanoscale mixing	311:347	facile and efficient nanoscale mixing with the CNFs	311:361	The polyampholytic character of Helux enabled facile and efficient nanoscale mixing with the CNFs, and the resulting composite mixtures of CNFs and Helux displayed thixotropic behavior and formed physical and reversibly cross-linked gels when left unperturbed for short spans of time.
32501668	2	17	with	mixing	342:347	arg1	CNFs					358:361	the CNFs	354:361	the CNFs	354:361	The polyampholytic character of Helux enabled facile and efficient nanoscale mixing with the CNFs, and the resulting composite mixtures of CNFs and Helux displayed thixotropic behavior and formed physical and reversibly cross-linked gels when left unperturbed for short spans of time.
32501668	2	18	theme	time	544:547	arg1	short					529:533	short	529:533	short	529:533	The polyampholytic character of Helux enabled facile and efficient nanoscale mixing with the CNFs, and the resulting composite mixtures of CNFs and Helux displayed thixotropic behavior and formed physical and reversibly cross-linked gels when left unperturbed for short spans of time.
32501668	8	19	contain	containing	1470:1479	arg1	polymer					1462:1468	a polymer	1460:1468	a polymer containing cationic groups	1460:1495	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups is unique, novel, and promising as a functional material platform.
32501668	8	19	contain	containing	1470:1479	arg2	groups					1490:1495	cationic groups	1481:1495	cationic groups	1481:1495	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups is unique, novel, and promising as a functional material platform.
32501668	1	20	from	nanofibrils	188:198	arg1	platform					95:102	A new platform	89:102	A new platform of functional hybrid materials from anionically charged high-aspect-ratio cellulose nanofibrils (CNFs) and a dendritic polyampholyte, Helux,	89:243	A new platform of functional hybrid materials from anionically charged high-aspect-ratio cellulose nanofibrils (CNFs) and a dendritic polyampholyte, Helux, is herein proposed.
32501668	1	21	theme	new	91:93	arg1	platform					95:102	A new platform	89:102	A new platform of functional hybrid materials from anionically charged high-aspect-ratio cellulose nanofibrils (CNFs) and a dendritic polyampholyte, Helux,	89:243	A new platform of functional hybrid materials from anionically charged high-aspect-ratio cellulose nanofibrils (CNFs) and a dendritic polyampholyte, Helux, is herein proposed.
32501668	2	22	theme	Helux	413:417	arg1	mixtures					392:399	the resulting composite mixtures	368:399	the resulting composite mixtures of CNFs and Helux	368:417	The polyampholytic character of Helux enabled facile and efficient nanoscale mixing with the CNFs, and the resulting composite mixtures of CNFs and Helux displayed thixotropic behavior and formed physical and reversibly cross-linked gels when left unperturbed for short spans of time.
32501668	2	23	theme	cross-linked	485:496	arg1	gels					498:501	physical and reversibly cross-linked gels	461:501	physical and reversibly cross-linked gels	461:501	The polyampholytic character of Helux enabled facile and efficient nanoscale mixing with the CNFs, and the resulting composite mixtures of CNFs and Helux displayed thixotropic behavior and formed physical and reversibly cross-linked gels when left unperturbed for short spans of time.
32501668	4	24	with	aerogels	838:845	arg1	densities					852:860	densities	852:860	densities as low as 4 kg/m3 by lyophilizing ice-templated CNF/Helux mixtures	852:927	Non-cross-linked mixtures of CNF/Helux were assembled into composites, such as films by solvent casting and aerogels with densities as low as 4 kg/m3 by lyophilizing ice-templated CNF/Helux mixtures.
32501668	4	25	theme	Non-cross-linked	730:745	arg1	mixtures					747:754	Non-cross-linked mixtures	730:754	Non-cross-linked mixtures of CNF/Helux	730:767	Non-cross-linked mixtures of CNF/Helux were assembled into composites, such as films by solvent casting and aerogels with densities as low as 4 kg/m3 by lyophilizing ice-templated CNF/Helux mixtures.
32501668	2	26	theme	Helux	297:301	arg1	character					284:292	The polyampholytic character	265:292	The polyampholytic character of Helux	265:301	The polyampholytic character of Helux enabled facile and efficient nanoscale mixing with the CNFs, and the resulting composite mixtures of CNFs and Helux displayed thixotropic behavior and formed physical and reversibly cross-linked gels when left unperturbed for short spans of time.
32501668	6	27	theme	mechanical	1162:1171	arg1	jeopardized					1206:1216	jeopardized	1206:1216	jeopardized	1206:1216	The mechanical performance of the films was not jeopardized by the addition of Helux.
32501668	6	27	theme	mechanical	1162:1171	arg1	performance					1173:1183	The mechanical performance	1158:1183	The mechanical performance of the films	1158:1196	The mechanical performance of the films was not jeopardized by the addition of Helux.
32501668	0	28	theme	Polyampholyte-Assisted	10:31	arg1	Formation					33:41	Dendritic Polyampholyte-Assisted Formation	0:41	Dendritic Polyampholyte-Assisted Formation of Functional Cellulose Nanofibril Materials	0:86	Dendritic Polyampholyte-Assisted Formation of Functional Cellulose Nanofibril Materials.
32501668	7	29	theme	non-cross-linked	1358:1373	arg1	states					1392:1397	both the non-cross-linked and cross-linked states	1349:1397	states	1392:1397	Additionally, by varying the amount of Helux, the compressive elastic modulus of aerogels was tunable in both the non-cross-linked and cross-linked states.
32501668	2	30	link	cross-linked	485:496	arg1	gels					498:501	physical and reversibly cross-linked gels	461:501	physical and reversibly cross-linked gels	461:501	The polyampholytic character of Helux enabled facile and efficient nanoscale mixing with the CNFs, and the resulting composite mixtures of CNFs and Helux displayed thixotropic behavior and formed physical and reversibly cross-linked gels when left unperturbed for short spans of time.
32501668	3	31	theme	self-supporting	596:610	arg1	hydrogels					618:626	self-supporting solid hydrogels	596:626	self-supporting solid hydrogels containing impressive water contents of 99.6% and a storage modulus of 1.8 kPa	596:705	The gel could be chemically cross-linked into self-supporting solid hydrogels containing impressive water contents of 99.6% and a storage modulus of 1.8 kPa by thermal activation.
32501668	2	32	theme	CNFs	404:407	arg1	mixtures					392:399	the resulting composite mixtures	368:399	the resulting composite mixtures of CNFs and Helux	368:417	The polyampholytic character of Helux enabled facile and efficient nanoscale mixing with the CNFs, and the resulting composite mixtures of CNFs and Helux displayed thixotropic behavior and formed physical and reversibly cross-linked gels when left unperturbed for short spans of time.
32501668	0	33	theme	Dendritic	0:8	arg1	Formation					33:41	Dendritic Polyampholyte-Assisted Formation	0:41	Dendritic Polyampholyte-Assisted Formation of Functional Cellulose Nanofibril Materials	0:86	Dendritic Polyampholyte-Assisted Formation of Functional Cellulose Nanofibril Materials.
32501668	4	34	theme	CNF/Helux	759:767	arg1	mixtures					747:754	Non-cross-linked mixtures	730:754	Non-cross-linked mixtures of CNF/Helux	730:767	Non-cross-linked mixtures of CNF/Helux were assembled into composites, such as films by solvent casting and aerogels with densities as low as 4 kg/m3 by lyophilizing ice-templated CNF/Helux mixtures.
32501668	1	35	theme	functional	107:116	arg1	materials					125:133	functional hybrid materials	107:133	functional hybrid materials	107:133	A new platform of functional hybrid materials from anionically charged high-aspect-ratio cellulose nanofibrils (CNFs) and a dendritic polyampholyte, Helux, is herein proposed.
32501668	1	36	theme	dendritic	213:221	arg1	Helux					238:242	Helux	238:242	Helux	238:242	A new platform of functional hybrid materials from anionically charged high-aspect-ratio cellulose nanofibrils (CNFs) and a dendritic polyampholyte, Helux, is herein proposed.
32501668	1	36	theme	dendritic	213:221	arg1	polyampholyte					223:235	a dendritic polyampholyte	211:235	a dendritic polyampholyte	211:235	A new platform of functional hybrid materials from anionically charged high-aspect-ratio cellulose nanofibrils (CNFs) and a dendritic polyampholyte, Helux, is herein proposed.
32501668	2	37	theme	physical	461:468	arg1	gels					498:501	physical and reversibly cross-linked gels	461:501	physical and reversibly cross-linked gels	461:501	The polyampholytic character of Helux enabled facile and efficient nanoscale mixing with the CNFs, and the resulting composite mixtures of CNFs and Helux displayed thixotropic behavior and formed physical and reversibly cross-linked gels when left unperturbed for short spans of time.
32501668	8	38	theme	cationic	1481:1488	arg1	groups					1490:1495	cationic groups	1481:1495	cationic groups	1481:1495	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups is unique, novel, and promising as a functional material platform.
32501668	1	39	theme	hybrid	118:123	arg1	materials					125:133	functional hybrid materials	107:133	functional hybrid materials	107:133	A new platform of functional hybrid materials from anionically charged high-aspect-ratio cellulose nanofibrils (CNFs) and a dendritic polyampholyte, Helux, is herein proposed.
32501668	5	40	theme	amidation	1085:1093	arg1	chemistry					1095:1103	amidation chemistry	1085:1103	amidation chemistry using Helux-accessible amines in aqueous conditions	1085:1155	The resulting materials exhibited excellent wet stability due to the heat-activated cross-linking and were readily available for postfunctionalization via amidation chemistry using Helux-accessible amines in aqueous conditions.
32501668	7	41	theme	cross-linked	1379:1390	arg1	states					1392:1397	both the non-cross-linked and cross-linked states	1349:1397	states	1392:1397	Additionally, by varying the amount of Helux, the compressive elastic modulus of aerogels was tunable in both the non-cross-linked and cross-linked states.
32501668	3	42	theme	solid	612:616	arg1	hydrogels					618:626	self-supporting solid hydrogels	596:626	self-supporting solid hydrogels containing impressive water contents of 99.6% and a storage modulus of 1.8 kPa	596:705	The gel could be chemically cross-linked into self-supporting solid hydrogels containing impressive water contents of 99.6% and a storage modulus of 1.8 kPa by thermal activation.
32501668	8	43	theme	polymer	1462:1468	arg1	mixing					1433:1438	The fast and efficient nanoscale mixing	1400:1438	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups	1400:1495	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups is unique, novel, and promising as a functional material platform.
32501668	8	43	theme	polymer	1462:1468	arg1	unique					1500:1505	unique	1500:1505	unique	1500:1505	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups is unique, novel, and promising as a functional material platform.
32501668	3	44	theme	storage	680:686	arg1	modulus					688:694	a storage modulus	678:694	a storage modulus of 1.8 kPa	678:705	The gel could be chemically cross-linked into self-supporting solid hydrogels containing impressive water contents of 99.6% and a storage modulus of 1.8 kPa by thermal activation.
32501668	1	45	theme	materials	125:133	arg1	platform					95:102	A new platform	89:102	A new platform of functional hybrid materials from anionically charged high-aspect-ratio cellulose nanofibrils (CNFs) and a dendritic polyampholyte, Helux,	89:243	A new platform of functional hybrid materials from anionically charged high-aspect-ratio cellulose nanofibrils (CNFs) and a dendritic polyampholyte, Helux, is herein proposed.
32501668	6	46	theme	Helux	1237:1241	arg1	addition					1225:1232	the addition	1221:1232	the addition of Helux	1221:1241	The mechanical performance of the films was not jeopardized by the addition of Helux.
32501668	0	47	theme	Cellulose	57:65	arg1	Materials					78:86	Functional Cellulose Nanofibril Materials	46:86	Functional Cellulose Nanofibril Materials	46:86	Dendritic Polyampholyte-Assisted Formation of Functional Cellulose Nanofibril Materials.
32501668	9	48	theme	Sustainable	1564:1574	arg1	CNFs					1576:1579	Sustainable CNFs	1564:1579	Sustainable CNFs guided by heterofunctional dendritic polyampholytes	1564:1631	Sustainable CNFs guided by heterofunctional dendritic polyampholytes are envisaged to act as a pillar toward high-performance applications, including biomedicine and biomaterials.
32501668	9	48	theme	Sustainable	1564:1574	arg1	pillar					1659:1664	a pillar	1657:1664	a pillar toward high-performance applications, including biomedicine and biomaterials	1657:1741	Sustainable CNFs guided by heterofunctional dendritic polyampholytes are envisaged to act as a pillar toward high-performance applications, including biomedicine and biomaterials.
32501668	7	49	theme	aerogels	1325:1332	arg1	modulus					1314:1320	the compressive elastic modulus	1290:1320	the compressive elastic modulus of aerogels	1290:1332	Additionally, by varying the amount of Helux, the compressive elastic modulus of aerogels was tunable in both the non-cross-linked and cross-linked states.
32501668	7	49	theme	aerogels	1325:1332	arg1	tunable					1338:1344	tunable	1338:1344	tunable	1338:1344	Additionally, by varying the amount of Helux, the compressive elastic modulus of aerogels was tunable in both the non-cross-linked and cross-linked states.
32501668	3	50	theme	impressive	639:648	arg1	contents					656:663	impressive water contents	639:663	impressive water contents of 99.6%	639:672	The gel could be chemically cross-linked into self-supporting solid hydrogels containing impressive water contents of 99.6% and a storage modulus of 1.8 kPa by thermal activation.
32501668	2	51	theme	thixotropic	429:439	arg1	behavior					441:448	thixotropic behavior	429:448	thixotropic behavior	429:448	The polyampholytic character of Helux enabled facile and efficient nanoscale mixing with the CNFs, and the resulting composite mixtures of CNFs and Helux displayed thixotropic behavior and formed physical and reversibly cross-linked gels when left unperturbed for short spans of time.
32501668	0	52	theme	Functional	46:55	arg1	Materials					78:86	Functional Cellulose Nanofibril Materials	46:86	Functional Cellulose Nanofibril Materials	46:86	Dendritic Polyampholyte-Assisted Formation of Functional Cellulose Nanofibril Materials.
32501668	3	53	theme	water	650:654	arg1	contents					656:663	impressive water contents	639:663	impressive water contents of 99.6%	639:672	The gel could be chemically cross-linked into self-supporting solid hydrogels containing impressive water contents of 99.6% and a storage modulus of 1.8 kPa by thermal activation.
32501668	0	54	theme	Materials	78:86	arg1	Formation					33:41	Dendritic Polyampholyte-Assisted Formation	0:41	Dendritic Polyampholyte-Assisted Formation of Functional Cellulose Nanofibril Materials	0:86	Dendritic Polyampholyte-Assisted Formation of Functional Cellulose Nanofibril Materials.
32501668	9	55	theme	dendritic	1608:1616	arg1	polyampholytes					1618:1631	heterofunctional dendritic polyampholytes	1591:1631	heterofunctional dendritic polyampholytes	1591:1631	Sustainable CNFs guided by heterofunctional dendritic polyampholytes are envisaged to act as a pillar toward high-performance applications, including biomedicine and biomaterials.
32501668	8	56	theme	CNFs	1451:1454	arg1	mixing					1433:1438	The fast and efficient nanoscale mixing	1400:1438	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups	1400:1495	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups is unique, novel, and promising as a functional material platform.
32501668	8	56	theme	CNFs	1451:1454	arg1	unique					1500:1505	unique	1500:1505	unique	1500:1505	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups is unique, novel, and promising as a functional material platform.
32501668	8	57	theme	functional	1534:1543	arg1	platform					1554:1561	a functional material platform	1532:1561	a functional material platform	1532:1561	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups is unique, novel, and promising as a functional material platform.
32501668	4	58	theme	low	865:867	arg1	densities					852:860	densities	852:860	densities as low as 4 kg/m3 by lyophilizing ice-templated CNF/Helux mixtures	852:927	Non-cross-linked mixtures of CNF/Helux were assembled into composites, such as films by solvent casting and aerogels with densities as low as 4 kg/m3 by lyophilizing ice-templated CNF/Helux mixtures.
32501668	8	59	theme	anionic	1443:1449	arg1	CNFs					1451:1454	anionic CNFs	1443:1454	anionic CNFs	1443:1454	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups is unique, novel, and promising as a functional material platform.
32501668	0	60	theme	Nanofibril	67:76	arg1	Materials					78:86	Functional Cellulose Nanofibril Materials	46:86	Functional Cellulose Nanofibril Materials	46:86	Dendritic Polyampholyte-Assisted Formation of Functional Cellulose Nanofibril Materials.
32501668	3	61	theme	kPa	703:705	arg1	modulus					688:694	a storage modulus	678:694	a storage modulus of 1.8 kPa	678:705	The gel could be chemically cross-linked into self-supporting solid hydrogels containing impressive water contents of 99.6% and a storage modulus of 1.8 kPa by thermal activation.
32501668	3	61	theme	kPa	703:705	arg1	contents					656:663	impressive water contents	639:663	impressive water contents of 99.6%	639:672	The gel could be chemically cross-linked into self-supporting solid hydrogels containing impressive water contents of 99.6% and a storage modulus of 1.8 kPa by thermal activation.
32501668	5	62	theme	Helux-accessible	1111:1126	arg1	amines					1128:1133	Helux-accessible amines	1111:1133	Helux-accessible amines	1111:1133	The resulting materials exhibited excellent wet stability due to the heat-activated cross-linking and were readily available for postfunctionalization via amidation chemistry using Helux-accessible amines in aqueous conditions.
32501668	1	63	from	polyampholyte	223:235	arg1	platform					95:102	A new platform	89:102	A new platform of functional hybrid materials from anionically charged high-aspect-ratio cellulose nanofibrils (CNFs) and a dendritic polyampholyte, Helux,	89:243	A new platform of functional hybrid materials from anionically charged high-aspect-ratio cellulose nanofibrils (CNFs) and a dendritic polyampholyte, Helux, is herein proposed.
32501668	5	64	theme	excellent	964:972	arg1	stability					978:986	excellent wet stability	964:986	excellent wet stability	964:986	The resulting materials exhibited excellent wet stability due to the heat-activated cross-linking and were readily available for postfunctionalization via amidation chemistry using Helux-accessible amines in aqueous conditions.
32501668	7	65	from	tunable	1338:1344	arg1	states					1392:1397	both the non-cross-linked and cross-linked states	1349:1397	states	1392:1397	Additionally, by varying the amount of Helux, the compressive elastic modulus of aerogels was tunable in both the non-cross-linked and cross-linked states.
32501668	7	66	from	states	1392:1397	arg1	modulus					1314:1320	the compressive elastic modulus	1290:1320	the compressive elastic modulus of aerogels	1290:1332	Additionally, by varying the amount of Helux, the compressive elastic modulus of aerogels was tunable in both the non-cross-linked and cross-linked states.
32501668	7	66	from	states	1392:1397	arg1	tunable					1338:1344	tunable	1338:1344	tunable	1338:1344	Additionally, by varying the amount of Helux, the compressive elastic modulus of aerogels was tunable in both the non-cross-linked and cross-linked states.
32501668	1	67	theme	charged	152:158	arg1	nanofibrils					188:198	anionically charged high-aspect-ratio cellulose nanofibrils	140:198	anionically charged high-aspect-ratio cellulose nanofibrils (CNFs)	140:205	A new platform of functional hybrid materials from anionically charged high-aspect-ratio cellulose nanofibrils (CNFs) and a dendritic polyampholyte, Helux, is herein proposed.
32501668	1	67	theme	charged	152:158	arg1	CNFs					201:204	CNFs	201:204	CNFs	201:204	A new platform of functional hybrid materials from anionically charged high-aspect-ratio cellulose nanofibrils (CNFs) and a dendritic polyampholyte, Helux, is herein proposed.
32501668	2	68	theme	nanoscale	332:340	arg1	mixing					342:347	facile and efficient nanoscale mixing	311:347	facile and efficient nanoscale mixing with the CNFs	311:361	The polyampholytic character of Helux enabled facile and efficient nanoscale mixing with the CNFs, and the resulting composite mixtures of CNFs and Helux displayed thixotropic behavior and formed physical and reversibly cross-linked gels when left unperturbed for short spans of time.
32501668	2	69	dep	short	529:533	arg1	spans					535:539	spans	535:539	spans	535:539	The polyampholytic character of Helux enabled facile and efficient nanoscale mixing with the CNFs, and the resulting composite mixtures of CNFs and Helux displayed thixotropic behavior and formed physical and reversibly cross-linked gels when left unperturbed for short spans of time.
32501668	5	70	theme	aqueous	1138:1144	arg1	conditions					1146:1155	aqueous conditions	1138:1155	aqueous conditions	1138:1155	The resulting materials exhibited excellent wet stability due to the heat-activated cross-linking and were readily available for postfunctionalization via amidation chemistry using Helux-accessible amines in aqueous conditions.
32501668	3	71	theme	%	672:672	arg1	modulus					688:694	a storage modulus	678:694	a storage modulus of 1.8 kPa	678:705	The gel could be chemically cross-linked into self-supporting solid hydrogels containing impressive water contents of 99.6% and a storage modulus of 1.8 kPa by thermal activation.
32501668	3	71	theme	%	672:672	arg1	contents					656:663	impressive water contents	639:663	impressive water contents of 99.6%	639:672	The gel could be chemically cross-linked into self-supporting solid hydrogels containing impressive water contents of 99.6% and a storage modulus of 1.8 kPa by thermal activation.
32501668	1	72	theme	high-aspect-ratio	160:176	arg1	nanofibrils					188:198	anionically charged high-aspect-ratio cellulose nanofibrils	140:198	anionically charged high-aspect-ratio cellulose nanofibrils (CNFs)	140:205	A new platform of functional hybrid materials from anionically charged high-aspect-ratio cellulose nanofibrils (CNFs) and a dendritic polyampholyte, Helux, is herein proposed.
32501668	1	72	theme	high-aspect-ratio	160:176	arg1	CNFs					201:204	CNFs	201:204	CNFs	201:204	A new platform of functional hybrid materials from anionically charged high-aspect-ratio cellulose nanofibrils (CNFs) and a dendritic polyampholyte, Helux, is herein proposed.
32501668	8	73	theme	efficient	1413:1421	arg1	mixing					1433:1438	The fast and efficient nanoscale mixing	1400:1438	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups	1400:1495	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups is unique, novel, and promising as a functional material platform.
32501668	8	73	theme	efficient	1413:1421	arg1	unique					1500:1505	unique	1500:1505	unique	1500:1505	The fast and efficient nanoscale mixing of anionic CNFs and a polymer containing cationic groups is unique, novel, and promising as a functional material platform.
32501668	5	74	theme	wet	974:976	arg1	stability					978:986	excellent wet stability	964:986	excellent wet stability	964:986	The resulting materials exhibited excellent wet stability due to the heat-activated cross-linking and were readily available for postfunctionalization via amidation chemistry using Helux-accessible amines in aqueous conditions.
32501668	7	75	theme	compressive	1294:1304	arg1	modulus					1314:1320	the compressive elastic modulus	1290:1320	the compressive elastic modulus of aerogels	1290:1332	Additionally, by varying the amount of Helux, the compressive elastic modulus of aerogels was tunable in both the non-cross-linked and cross-linked states.
32501668	7	75	theme	compressive	1294:1304	arg1	tunable					1338:1344	tunable	1338:1344	tunable	1338:1344	Additionally, by varying the amount of Helux, the compressive elastic modulus of aerogels was tunable in both the non-cross-linked and cross-linked states.
32501668	6	76	theme	films	1192:1196	arg1	jeopardized					1206:1216	jeopardized	1206:1216	jeopardized	1206:1216	The mechanical performance of the films was not jeopardized by the addition of Helux.
32501668	6	76	theme	films	1192:1196	arg1	performance					1173:1183	The mechanical performance	1158:1183	The mechanical performance of the films	1158:1196	The mechanical performance of the films was not jeopardized by the addition of Helux.
32501668	2	77	theme	polyampholytic	269:282	arg1	character					284:292	The polyampholytic character	265:292	The polyampholytic character of Helux	265:301	The polyampholytic character of Helux enabled facile and efficient nanoscale mixing with the CNFs, and the resulting composite mixtures of CNFs and Helux displayed thixotropic behavior and formed physical and reversibly cross-linked gels when left unperturbed for short spans of time.
32501668	1	78	theme	cellulose	178:186	arg1	nanofibrils					188:198	anionically charged high-aspect-ratio cellulose nanofibrils	140:198	anionically charged high-aspect-ratio cellulose nanofibrils (CNFs)	140:205	A new platform of functional hybrid materials from anionically charged high-aspect-ratio cellulose nanofibrils (CNFs) and a dendritic polyampholyte, Helux, is herein proposed.
32501668	1	78	theme	cellulose	178:186	arg1	CNFs					201:204	CNFs	201:204	CNFs	201:204	A new platform of functional hybrid materials from anionically charged high-aspect-ratio cellulose nanofibrils (CNFs) and a dendritic polyampholyte, Helux, is herein proposed.
32501668	9	79	theme	high-performance	1673:1688	arg1	biomaterials					1730:1741	biomaterials	1730:1741	biomaterials	1730:1741	Sustainable CNFs guided by heterofunctional dendritic polyampholytes are envisaged to act as a pillar toward high-performance applications, including biomedicine and biomaterials.
32501668	9	79	theme	high-performance	1673:1688	arg1	applications					1690:1701	high-performance applications	1673:1701	high-performance applications	1673:1701	Sustainable CNFs guided by heterofunctional dendritic polyampholytes are envisaged to act as a pillar toward high-performance applications, including biomedicine and biomaterials.
32501668	9	79	theme	high-performance	1673:1688	arg1	biomedicine					1714:1724	biomedicine	1714:1724	biomedicine	1714:1724	Sustainable CNFs guided by heterofunctional dendritic polyampholytes are envisaged to act as a pillar toward high-performance applications, including biomedicine and biomaterials.
32501668	7	80	theme	elastic	1306:1312	arg1	modulus					1314:1320	the compressive elastic modulus	1290:1320	the compressive elastic modulus of aerogels	1290:1332	Additionally, by varying the amount of Helux, the compressive elastic modulus of aerogels was tunable in both the non-cross-linked and cross-linked states.
32501668	7	80	theme	elastic	1306:1312	arg1	tunable					1338:1344	tunable	1338:1344	tunable	1338:1344	Additionally, by varying the amount of Helux, the compressive elastic modulus of aerogels was tunable in both the non-cross-linked and cross-linked states.
32501668	7	81	dep	states	1392:1397	arg1	both					1349:1352	both	1349:1352	both	1349:1352	Additionally, by varying the amount of Helux, the compressive elastic modulus of aerogels was tunable in both the non-cross-linked and cross-linked states.
32501668	2	82	theme	composite	382:390	arg1	mixtures					392:399	the resulting composite mixtures	368:399	the resulting composite mixtures of CNFs and Helux	368:417	The polyampholytic character of Helux enabled facile and efficient nanoscale mixing with the CNFs, and the resulting composite mixtures of CNFs and Helux displayed thixotropic behavior and formed physical and reversibly cross-linked gels when left unperturbed for short spans of time.
32501668	9	83	theme	heterofunctional	1591:1606	arg1	polyampholytes					1618:1631	heterofunctional dendritic polyampholytes	1591:1631	heterofunctional dendritic polyampholytes	1591:1631	Sustainable CNFs guided by heterofunctional dendritic polyampholytes are envisaged to act as a pillar toward high-performance applications, including biomedicine and biomaterials.
32501668	7	84	theme	Helux	1283:1287	arg1	Helux					1283:1287	Helux	1283:1287	Helux	1283:1287	Additionally, by varying the amount of Helux, the compressive elastic modulus of aerogels was tunable in both the non-cross-linked and cross-linked states.
32501668	7	84	theme	Helux	1283:1287	arg1	amount					1273:1278	the amount	1269:1278	the amount of Helux	1269:1287	Additionally, by varying the amount of Helux, the compressive elastic modulus of aerogels was tunable in both the non-cross-linked and cross-linked states.
34182368	4	0	theme	statistical	504:514	arg1	methods					516:522	multivariate statistical methods	491:522	multivariate statistical methods	491:522	The microbial communities of V3 and V4 region 16S rRNA gene was amplified by PCR, then sequenced on an Illumina MiSeq PE250 platform and analyzed by multivariate statistical methods.
34182368	3	1	theme	pulp	250:253	arg1	JFP-Ps					256:261	pulp (JFP-Ps)	250:262	pulp (JFP-Ps) on gut microbiota composition and short-chain fatty acids	250:320	pulp (JFP-Ps) on gut microbiota composition and short-chain fatty acids production in mice.
34182368	4	2	theme	gene	397:400	arg1	communities					356:366	The microbial communities	342:366	The microbial communities of V3 and V4 region 16S rRNA gene	342:400	The microbial communities of V3 and V4 region 16S rRNA gene was amplified by PCR, then sequenced on an Illumina MiSeq PE250 platform and analyzed by multivariate statistical methods.
34182368	4	3	theme	multivariate	491:502	arg1	methods					516:522	multivariate statistical methods	491:522	multivariate statistical methods	491:522	The microbial communities of V3 and V4 region 16S rRNA gene was amplified by PCR, then sequenced on an Illumina MiSeq PE250 platform and analyzed by multivariate statistical methods.
34182368	5	4	theme	flame	639:643	arg1	FID					666:668	FID	666:668	FID	666:668	The concentrations of short-chain fatty acids (SCFAs) were measured using gas chromatography (GC) equipped with a flame ionization detector (FID).
34182368	5	4	theme	flame	639:643	arg1	detector					656:663	a flame ionization detector	637:663	a flame ionization detector (FID)	637:669	The concentrations of short-chain fatty acids (SCFAs) were measured using gas chromatography (GC) equipped with a flame ionization detector (FID).
34182368	4	5	theme	rRNA	392:395	arg1	gene					397:400	V3 and V4 region 16S rRNA gene	371:400	V3 and V4 region 16S rRNA gene	371:400	The microbial communities of V3 and V4 region 16S rRNA gene was amplified by PCR, then sequenced on an Illumina MiSeq PE250 platform and analyzed by multivariate statistical methods.
34182368	7	6	from	concentrations	887:900	arg1	feces					974:978	mouse feces	968:978	mouse feces	968:978	The concentrations of acetic acid, propionic acid, n-butyric acid and total SCFAs in mouse feces were significantly increased by treatment with JFP-Ps for 2 weeks.
34182368	5	7	theme	ionization	645:654	arg1	FID					666:668	FID	666:668	FID	666:668	The concentrations of short-chain fatty acids (SCFAs) were measured using gas chromatography (GC) equipped with a flame ionization detector (FID).
34182368	5	7	theme	ionization	645:654	arg1	detector					656:663	a flame ionization detector	637:663	a flame ionization detector (FID)	637:669	The concentrations of short-chain fatty acids (SCFAs) were measured using gas chromatography (GC) equipped with a flame ionization detector (FID).
34182368	5	8	theme	gas	599:601	arg1	GC					619:620	GC	619:620	GC	619:620	The concentrations of short-chain fatty acids (SCFAs) were measured using gas chromatography (GC) equipped with a flame ionization detector (FID).
34182368	5	8	theme	gas	599:601	arg1	chromatography					603:616	gas chromatography	599:616	gas chromatography (GC) equipped with a flame ionization detector (FID)	599:669	The concentrations of short-chain fatty acids (SCFAs) were measured using gas chromatography (GC) equipped with a flame ionization detector (FID).
34182368	6	9	theme	bacteria	751:758	arg1	levels					730:735	the levels	726:735	the levels of intestinal bacteria, including Bacteroidetes, Firmicutes, Proteobacteria, Cyanobacteria, Actinobacteria, Tenericutes, Deferribacteres and TM7	726:880	The results showed that JFP-Ps significantly affected the levels of intestinal bacteria, including Bacteroidetes, Firmicutes, Proteobacteria, Cyanobacteria, Actinobacteria, Tenericutes, Deferribacteres and TM7.
34182368	2	10	from	Lam	245:247	arg1	effects					189:195	the effects	185:195	the effects of polysaccharide from Artocarpus heterophyllus Lam	185:247	This study aimed to investigate the effects of polysaccharide from Artocarpus heterophyllus Lam.
34182368	8	11	theme	functional	1140:1149	arg1	JFP-Ps					1075:1080	JFP-Ps	1075:1080	JFP-Ps	1075:1080	These results indicate that JFP-Ps is beneficial to the gut health and can be developed as a functional ingredient in relation to gut health.
34182368	8	11	theme	functional	1140:1149	arg1	ingredient					1151:1160	a functional ingredient	1138:1160	a functional ingredient in relation to gut health	1138:1186	These results indicate that JFP-Ps is beneficial to the gut health and can be developed as a functional ingredient in relation to gut health.
34182368	4	12	theme	MiSeq	454:458	arg1	platform					466:473	an Illumina MiSeq PE250 platform	442:473	an Illumina MiSeq PE250 platform	442:473	The microbial communities of V3 and V4 region 16S rRNA gene was amplified by PCR, then sequenced on an Illumina MiSeq PE250 platform and analyzed by multivariate statistical methods.
34182368	7	13	theme	acid	944:947	arg1	concentrations					887:900	The concentrations	883:900	The concentrations of acetic acid, propionic acid, n-butyric acid and total SCFAs in mouse feces	883:978	The concentrations of acetic acid, propionic acid, n-butyric acid and total SCFAs in mouse feces were significantly increased by treatment with JFP-Ps for 2 weeks.
34182368	7	14	with	treatment	1012:1020	arg1	JFP-Ps					1027:1032	JFP-Ps	1027:1032	JFP-Ps for 2 weeks	1027:1044	The concentrations of acetic acid, propionic acid, n-butyric acid and total SCFAs in mouse feces were significantly increased by treatment with JFP-Ps for 2 weeks.
34182368	4	15	theme	V4	378:379	arg1	gene					397:400	V3 and V4 region 16S rRNA gene	371:400	V3 and V4 region 16S rRNA gene	371:400	The microbial communities of V3 and V4 region 16S rRNA gene was amplified by PCR, then sequenced on an Illumina MiSeq PE250 platform and analyzed by multivariate statistical methods.
34182368	1	16	theme	gut	77:79	arg1	composition					92:102	gut microbiota composition	77:102	gut microbiota composition	77:102	(jackfruit) pulp modulates gut microbiota composition and improves short-chain fatty acids production.
34182368	7	17	theme	total	953:957	arg1	SCFAs					959:963	total SCFAs	953:963	total SCFAs	953:963	The concentrations of acetic acid, propionic acid, n-butyric acid and total SCFAs in mouse feces were significantly increased by treatment with JFP-Ps for 2 weeks.
34182368	7	18	theme	n-butyric	934:942	arg1	acid					944:947	n-butyric acid	934:947	n-butyric acid	934:947	The concentrations of acetic acid, propionic acid, n-butyric acid and total SCFAs in mouse feces were significantly increased by treatment with JFP-Ps for 2 weeks.
34182368	7	19	theme	propionic	918:926	arg1	acid					928:931	propionic acid	918:931	propionic acid	918:931	The concentrations of acetic acid, propionic acid, n-butyric acid and total SCFAs in mouse feces were significantly increased by treatment with JFP-Ps for 2 weeks.
34182368	1	20	theme	microbiota	81:90	arg1	composition					92:102	gut microbiota composition	77:102	gut microbiota composition	77:102	(jackfruit) pulp modulates gut microbiota composition and improves short-chain fatty acids production.
34182368	7	21	theme	acid	928:931	arg1	concentrations					887:900	The concentrations	883:900	The concentrations of acetic acid, propionic acid, n-butyric acid and total SCFAs in mouse feces	883:978	The concentrations of acetic acid, propionic acid, n-butyric acid and total SCFAs in mouse feces were significantly increased by treatment with JFP-Ps for 2 weeks.
34182368	4	22	theme	16S	388:390	arg1	gene					397:400	V3 and V4 region 16S rRNA gene	371:400	V3 and V4 region 16S rRNA gene	371:400	The microbial communities of V3 and V4 region 16S rRNA gene was amplified by PCR, then sequenced on an Illumina MiSeq PE250 platform and analyzed by multivariate statistical methods.
34182368	0	23	theme	heterophyllus	31:43	arg1	Lam					45:47	Artocarpus heterophyllus Lam	20:47	Artocarpus heterophyllus Lam	20:47	Polysaccharide from Artocarpus heterophyllus Lam.
34182368	3	24	from	JFP-Ps	256:261	arg1	composition					282:292	gut microbiota composition	267:292	gut microbiota composition	267:292	pulp (JFP-Ps) on gut microbiota composition and short-chain fatty acids production in mice.
34182368	3	24	from	JFP-Ps	256:261	arg1	acids					316:320	short-chain fatty acids	298:320	short-chain fatty acids	298:320	pulp (JFP-Ps) on gut microbiota composition and short-chain fatty acids production in mice.
34182368	4	25	theme	region	381:386	arg1	gene					397:400	V3 and V4 region 16S rRNA gene	371:400	V3 and V4 region 16S rRNA gene	371:400	The microbial communities of V3 and V4 region 16S rRNA gene was amplified by PCR, then sequenced on an Illumina MiSeq PE250 platform and analyzed by multivariate statistical methods.
34182368	0	26	theme	Artocarpus	20:29	arg1	Lam					45:47	Artocarpus heterophyllus Lam	20:47	Artocarpus heterophyllus Lam	20:47	Polysaccharide from Artocarpus heterophyllus Lam.
34182368	5	27	theme	fatty	559:563	arg1	SCFAs					572:576	SCFAs	572:576	SCFAs	572:576	The concentrations of short-chain fatty acids (SCFAs) were measured using gas chromatography (GC) equipped with a flame ionization detector (FID).
34182368	5	27	theme	fatty	559:563	arg1	acids					565:569	short-chain fatty acids	547:569	short-chain fatty acids (SCFAs)	547:577	The concentrations of short-chain fatty acids (SCFAs) were measured using gas chromatography (GC) equipped with a flame ionization detector (FID).
34182368	6	28	dep	Proteobacteria	798:811	arg1	Firmicutes					786:795	Firmicutes	786:795	Firmicutes	786:795	The results showed that JFP-Ps significantly affected the levels of intestinal bacteria, including Bacteroidetes, Firmicutes, Proteobacteria, Cyanobacteria, Actinobacteria, Tenericutes, Deferribacteres and TM7.
34182368	3	29	theme	gut	267:269	arg1	composition					282:292	gut microbiota composition	267:292	gut microbiota composition	267:292	pulp (JFP-Ps) on gut microbiota composition and short-chain fatty acids production in mice.
34182368	8	30	theme	gut	1177:1179	arg1	health					1181:1186	gut health	1177:1186	gut health	1177:1186	These results indicate that JFP-Ps is beneficial to the gut health and can be developed as a functional ingredient in relation to gut health.
34182368	3	31	theme	microbiota	271:280	arg1	composition					282:292	gut microbiota composition	267:292	gut microbiota composition	267:292	pulp (JFP-Ps) on gut microbiota composition and short-chain fatty acids production in mice.
34182368	6	32	theme	intestinal	740:749	arg1	bacteria					751:758	intestinal bacteria	740:758	intestinal bacteria	740:758	The results showed that JFP-Ps significantly affected the levels of intestinal bacteria, including Bacteroidetes, Firmicutes, Proteobacteria, Cyanobacteria, Actinobacteria, Tenericutes, Deferribacteres and TM7.
34182368	6	32	theme	intestinal	740:749	arg1	Proteobacteria					798:811	Bacteroidetes, Firmicutes, Proteobacteria	771:811	Proteobacteria	798:811	The results showed that JFP-Ps significantly affected the levels of intestinal bacteria, including Bacteroidetes, Firmicutes, Proteobacteria, Cyanobacteria, Actinobacteria, Tenericutes, Deferribacteres and TM7.
34182368	5	33	theme	short-chain	547:557	arg1	SCFAs					572:576	SCFAs	572:576	SCFAs	572:576	The concentrations of short-chain fatty acids (SCFAs) were measured using gas chromatography (GC) equipped with a flame ionization detector (FID).
34182368	5	33	theme	short-chain	547:557	arg1	acids					565:569	short-chain fatty acids	547:569	short-chain fatty acids (SCFAs)	547:577	The concentrations of short-chain fatty acids (SCFAs) were measured using gas chromatography (GC) equipped with a flame ionization detector (FID).
34182368	4	34	theme	microbial	346:354	arg1	communities					356:366	The microbial communities	342:366	The microbial communities of V3 and V4 region 16S rRNA gene	342:400	The microbial communities of V3 and V4 region 16S rRNA gene was amplified by PCR, then sequenced on an Illumina MiSeq PE250 platform and analyzed by multivariate statistical methods.
34182368	1	35	theme	short-chain	117:127	arg1	acids					135:139	short-chain fatty acids	117:139	short-chain fatty acids production	117:150	(jackfruit) pulp modulates gut microbiota composition and improves short-chain fatty acids production.
34182368	8	36	from	ingredient	1151:1160	arg1	relation					1165:1172	relation	1165:1172	relation to gut health	1165:1186	These results indicate that JFP-Ps is beneficial to the gut health and can be developed as a functional ingredient in relation to gut health.
34182368	2	37	theme	Artocarpus	220:229	arg1	Lam					245:247	Artocarpus heterophyllus Lam	220:247	Artocarpus heterophyllus Lam	220:247	This study aimed to investigate the effects of polysaccharide from Artocarpus heterophyllus Lam.
34182368	2	38	theme	heterophyllus	231:243	arg1	Lam					245:247	Artocarpus heterophyllus Lam	220:247	Artocarpus heterophyllus Lam	220:247	This study aimed to investigate the effects of polysaccharide from Artocarpus heterophyllus Lam.
34182368	4	39	theme	V3	371:372	arg1	gene					397:400	V3 and V4 region 16S rRNA gene	371:400	V3 and V4 region 16S rRNA gene	371:400	The microbial communities of V3 and V4 region 16S rRNA gene was amplified by PCR, then sequenced on an Illumina MiSeq PE250 platform and analyzed by multivariate statistical methods.
34182368	1	40	theme	fatty	129:133	arg1	acids					135:139	short-chain fatty acids	117:139	short-chain fatty acids production	117:150	(jackfruit) pulp modulates gut microbiota composition and improves short-chain fatty acids production.
34182368	7	41	theme	acetic	905:910	arg1	acid					912:915	acetic acid	905:915	acetic acid	905:915	The concentrations of acetic acid, propionic acid, n-butyric acid and total SCFAs in mouse feces were significantly increased by treatment with JFP-Ps for 2 weeks.
34182368	0	42	dep	Polysaccharide	0:13	arg1	Lam					45:47	Artocarpus heterophyllus Lam	20:47	Artocarpus heterophyllus Lam	20:47	Polysaccharide from Artocarpus heterophyllus Lam.
34182368	3	43	theme	short-chain	298:308	arg1	acids					316:320	short-chain fatty acids	298:320	short-chain fatty acids	298:320	pulp (JFP-Ps) on gut microbiota composition and short-chain fatty acids production in mice.
34182368	6	44	theme	Bacteroidetes	771:783	arg1	Actinobacteria					829:842	Actinobacteria	829:842	Actinobacteria	829:842	The results showed that JFP-Ps significantly affected the levels of intestinal bacteria, including Bacteroidetes, Firmicutes, Proteobacteria, Cyanobacteria, Actinobacteria, Tenericutes, Deferribacteres and TM7.
34182368	6	44	theme	Bacteroidetes	771:783	arg1	Deferribacteres					858:872	Deferribacteres	858:872	Deferribacteres	858:872	The results showed that JFP-Ps significantly affected the levels of intestinal bacteria, including Bacteroidetes, Firmicutes, Proteobacteria, Cyanobacteria, Actinobacteria, Tenericutes, Deferribacteres and TM7.
34182368	6	44	theme	Bacteroidetes	771:783	arg1	Cyanobacteria					814:826	Cyanobacteria	814:826	Cyanobacteria	814:826	The results showed that JFP-Ps significantly affected the levels of intestinal bacteria, including Bacteroidetes, Firmicutes, Proteobacteria, Cyanobacteria, Actinobacteria, Tenericutes, Deferribacteres and TM7.
34182368	6	44	theme	Bacteroidetes	771:783	arg1	TM7					878:880	TM7	878:880	TM7	878:880	The results showed that JFP-Ps significantly affected the levels of intestinal bacteria, including Bacteroidetes, Firmicutes, Proteobacteria, Cyanobacteria, Actinobacteria, Tenericutes, Deferribacteres and TM7.
34182368	6	44	theme	Bacteroidetes	771:783	arg1	Proteobacteria					798:811	Bacteroidetes, Firmicutes, Proteobacteria	771:811	Proteobacteria	798:811	The results showed that JFP-Ps significantly affected the levels of intestinal bacteria, including Bacteroidetes, Firmicutes, Proteobacteria, Cyanobacteria, Actinobacteria, Tenericutes, Deferribacteres and TM7.
34182368	8	45	theme	gut	1103:1105	arg1	health					1107:1112	the gut health	1099:1112	the gut health	1099:1112	These results indicate that JFP-Ps is beneficial to the gut health and can be developed as a functional ingredient in relation to gut health.
34182368	5	46	theme	acids	565:569	arg1	concentrations					529:542	The concentrations	525:542	The concentrations of short-chain fatty acids (SCFAs)	525:577	The concentrations of short-chain fatty acids (SCFAs) were measured using gas chromatography (GC) equipped with a flame ionization detector (FID).
34182368	1	47	theme	acids	135:139	arg1	production					141:150	short-chain fatty acids production	117:150	short-chain fatty acids production	117:150	(jackfruit) pulp modulates gut microbiota composition and improves short-chain fatty acids production.
34182368	7	48	theme	acid	912:915	arg1	concentrations					887:900	The concentrations	883:900	The concentrations of acetic acid, propionic acid, n-butyric acid and total SCFAs in mouse feces	883:978	The concentrations of acetic acid, propionic acid, n-butyric acid and total SCFAs in mouse feces were significantly increased by treatment with JFP-Ps for 2 weeks.
34182368	3	49	theme	fatty	310:314	arg1	acids					316:320	short-chain fatty acids	298:320	short-chain fatty acids	298:320	pulp (JFP-Ps) on gut microbiota composition and short-chain fatty acids production in mice.
34182368	2	50	theme	polysaccharide	200:213	arg1	effects					189:195	the effects	185:195	the effects of polysaccharide from Artocarpus heterophyllus Lam	185:247	This study aimed to investigate the effects of polysaccharide from Artocarpus heterophyllus Lam.
34182368	7	51	theme	mouse	968:972	arg1	feces					974:978	mouse feces	968:978	mouse feces	968:978	The concentrations of acetic acid, propionic acid, n-butyric acid and total SCFAs in mouse feces were significantly increased by treatment with JFP-Ps for 2 weeks.
34182368	1	52	theme	jackfruit	51:59	arg1	pulp					62:65	(jackfruit) pulp	50:65	(jackfruit) pulp	50:65	(jackfruit) pulp modulates gut microbiota composition and improves short-chain fatty acids production.
34182368	7	53	theme	SCFAs	959:963	arg1	concentrations					887:900	The concentrations	883:900	The concentrations of acetic acid, propionic acid, n-butyric acid and total SCFAs in mouse feces	883:978	The concentrations of acetic acid, propionic acid, n-butyric acid and total SCFAs in mouse feces were significantly increased by treatment with JFP-Ps for 2 weeks.
34182368	3	54	from	production	322:331	arg1	mice					336:339	mice	336:339	mice	336:339	pulp (JFP-Ps) on gut microbiota composition and short-chain fatty acids production in mice.
33838188	0	0	theme	aerogel	80:86	arg1	material					22:29	A new cancellous bone material	0:29	A new cancellous bone material of silk fibroin/cellulose dual network composite aerogel	0:86	A new cancellous bone material of silk fibroin/cellulose dual network composite aerogel reinforced by nano-hydroxyapatite filler.
33838188	4	1	theme	alkali	544:549	arg1	system					556:561	an alkali urea system	541:561	an alkali urea system	541:561	In this work, an alkali urea system was used to dissolve, regenerate and gelate cellulose and silk fibroin (SF) to prepare composite aerosol.
33838188	5	2	theme	dual	671:674	arg1	structure					684:692	A dual network structure	669:692	A dual network structure	669:692	A dual network structure was shaped in the composite aerosol materials interlaced by sheet-like SF and reticular cellulose wrapping n-HA on the surface.
33838188	7	3	theme	mechanical	1044:1053	arg1	range					1064:1068	the same mechanical strength range	1035:1068	the same mechanical strength range	1035:1068	Our work indicates that the composite aerogel has the same mechanical strength range as cancellous bone when the ratio of cellulose, n-HA and SF being 8:1:1.
33838188	1	4	theme	aerogel	177:183	arg1	matrices					185:192	biopolymeric aerogel matrices	164:192	biopolymeric aerogel matrices	164:192	Composites materials comprised of biopolymeric aerogel matrices and inorganic nano-hydroxyapatite (n-HA) fillers have received considerable attention in bone engineering.
33838188	1	5	theme	bone	283:286	arg1	engineering					288:298	bone engineering	283:298	bone engineering	283:298	Composites materials comprised of biopolymeric aerogel matrices and inorganic nano-hydroxyapatite (n-HA) fillers have received considerable attention in bone engineering.
33838188	0	6	theme	composite	70:78	arg1	aerogel					80:86	silk fibroin/cellulose dual network composite aerogel	34:86	silk fibroin/cellulose dual network composite aerogel	34:86	A new cancellous bone material of silk fibroin/cellulose dual network composite aerogel reinforced by nano-hydroxyapatite filler.
33838188	6	7	theme	bone	930:933	arg1	one					915:917	the one	911:917	the one of natural bone	911:933	Through uniaxial compression, the density of the composite aerogel material was close to the one of natural bone, and mechanical strength and toughness were high.
33838188	6	7	theme	bone	930:933	arg1	density					856:862	the density	852:862	the density of the composite aerogel material	852:896	Through uniaxial compression, the density of the composite aerogel material was close to the one of natural bone, and mechanical strength and toughness were high.
33838188	5	8	theme	network	676:682	arg1	structure					684:692	A dual network structure	669:692	A dual network structure	669:692	A dual network structure was shaped in the composite aerosol materials interlaced by sheet-like SF and reticular cellulose wrapping n-HA on the surface.
33838188	5	9	from	SF	765:766	arg1	surface					813:819	the surface	809:819	the surface	809:819	A dual network structure was shaped in the composite aerosol materials interlaced by sheet-like SF and reticular cellulose wrapping n-HA on the surface.
33838188	8	10	dep	showed	1165:1170	arg1	had					1218:1220	had	1218:1220	showed HEK-293T cells cultured on composite aerogels had high ability of adhesion, proliferation and differentiation	1165:1280	In vitro cell culture showed HEK-293T cells cultured on composite aerogels had high ability of adhesion, proliferation and differentiation.
33838188	5	11	theme	sheet-like	754:763	arg1	SF					765:766	sheet-like SF	754:766	sheet-like SF	754:766	A dual network structure was shaped in the composite aerosol materials interlaced by sheet-like SF and reticular cellulose wrapping n-HA on the surface.
33838188	6	12	theme	natural	922:928	arg1	bone					930:933	natural bone	922:933	natural bone	922:933	Through uniaxial compression, the density of the composite aerogel material was close to the one of natural bone, and mechanical strength and toughness were high.
33838188	9	13	theme	presented	1296:1304	arg1	aerogel					1330:1336	the presented biodegradable composite aerogel	1292:1336	the presented biodegradable composite aerogel	1292:1336	Totally, the presented biodegradable composite aerogel has application potential in bone tissue engineering.
33838188	9	14	theme	biodegradable	1306:1318	arg1	aerogel					1330:1336	the presented biodegradable composite aerogel	1292:1336	the presented biodegradable composite aerogel	1292:1336	Totally, the presented biodegradable composite aerogel has application potential in bone tissue engineering.
33838188	7	15	theme	n-HA	1118:1121	arg1	ratio					1098:1102	the ratio	1094:1102	the ratio of cellulose, n-HA and SF	1094:1128	Our work indicates that the composite aerogel has the same mechanical strength range as cancellous bone when the ratio of cellulose, n-HA and SF being 8:1:1.
33838188	3	16	from	improvements	428:439	arg1	toughness					444:452	toughness	444:452	toughness	444:452	The improvements in toughness and mechanical strength of aerogel-based biomaterials are in great need.
33838188	3	16	from	improvements	428:439	arg1	strength					469:476	mechanical strength	458:476	mechanical strength	458:476	The improvements in toughness and mechanical strength of aerogel-based biomaterials are in great need.
33838188	8	17	dep	In	1143:1144	arg1	vitro					1146:1150	vitro	1146:1150	vitro	1146:1150	In vitro cell culture showed HEK-293T cells cultured on composite aerogels had high ability of adhesion, proliferation and differentiation.
33838188	6	18	theme	uniaxial	830:837	arg1	compression					839:849	uniaxial compression	830:849	uniaxial compression	830:849	Through uniaxial compression, the density of the composite aerogel material was close to the one of natural bone, and mechanical strength and toughness were high.
33838188	2	19	theme	significant	315:325	arg1	progress					327:334	significant progress	315:334	significant progress in aerogel-based biomaterials	315:364	Although with significant progress in aerogel-based biomaterials, the brittleness and low strengths limit the application.
33838188	3	20	theme	aerogel-based	481:493	arg1	biomaterials					495:506	aerogel-based biomaterials	481:506	aerogel-based biomaterials	481:506	The improvements in toughness and mechanical strength of aerogel-based biomaterials are in great need.
33838188	0	21	theme	nano-hydroxyapatite	102:120	arg1	filler					122:127	nano-hydroxyapatite filler	102:127	nano-hydroxyapatite filler	102:127	A new cancellous bone material of silk fibroin/cellulose dual network composite aerogel reinforced by nano-hydroxyapatite filler.
33838188	4	22	theme	silk	621:624	arg1	fibroin					626:632	silk fibroin	621:632	silk fibroin	621:632	In this work, an alkali urea system was used to dissolve, regenerate and gelate cellulose and silk fibroin (SF) to prepare composite aerosol.
33838188	6	23	theme	aerogel	881:887	arg1	material					889:896	the composite aerogel material	867:896	the composite aerogel material	867:896	Through uniaxial compression, the density of the composite aerogel material was close to the one of natural bone, and mechanical strength and toughness were high.
33838188	1	24	theme	inorganic	198:206	arg1	fillers					235:241	inorganic nano-hydroxyapatite (n-HA) fillers	198:241	inorganic nano-hydroxyapatite (n-HA) fillers	198:241	Composites materials comprised of biopolymeric aerogel matrices and inorganic nano-hydroxyapatite (n-HA) fillers have received considerable attention in bone engineering.
33838188	0	25	theme	new	2:4	arg1	material					22:29	A new cancellous bone material	0:29	A new cancellous bone material of silk fibroin/cellulose dual network composite aerogel	0:86	A new cancellous bone material of silk fibroin/cellulose dual network composite aerogel reinforced by nano-hydroxyapatite filler.
33838188	9	26	theme	bone	1367:1370	arg1	engineering					1379:1389	bone tissue engineering	1367:1389	bone tissue engineering	1367:1389	Totally, the presented biodegradable composite aerogel has application potential in bone tissue engineering.
33838188	4	27	used	used	567:570	arg2	system					556:561	an alkali urea system	541:561	an alkali urea system	541:561	In this work, an alkali urea system was used to dissolve, regenerate and gelate cellulose and silk fibroin (SF) to prepare composite aerosol.
33838188	5	28	theme	cellulose	782:790	arg1	n-HA					801:804	reticular cellulose wrapping n-HA	772:804	reticular cellulose wrapping n-HA	772:804	A dual network structure was shaped in the composite aerosol materials interlaced by sheet-like SF and reticular cellulose wrapping n-HA on the surface.
33838188	3	29	theme	mechanical	458:467	arg1	strength					469:476	mechanical strength	458:476	mechanical strength	458:476	The improvements in toughness and mechanical strength of aerogel-based biomaterials are in great need.
33838188	9	30	theme	composite	1320:1328	arg1	aerogel					1330:1336	the presented biodegradable composite aerogel	1292:1336	the presented biodegradable composite aerogel	1292:1336	Totally, the presented biodegradable composite aerogel has application potential in bone tissue engineering.
33838188	1	31	theme	nano-hydroxyapatite	208:226	arg1	fillers					235:241	inorganic nano-hydroxyapatite (n-HA) fillers	198:241	inorganic nano-hydroxyapatite (n-HA) fillers	198:241	Composites materials comprised of biopolymeric aerogel matrices and inorganic nano-hydroxyapatite (n-HA) fillers have received considerable attention in bone engineering.
33838188	8	32	theme	HEK-293T	1172:1179	arg1	cells					1181:1185	HEK-293T cells	1172:1185	HEK-293T cells cultured on composite aerogels	1172:1216	In vitro cell culture showed HEK-293T cells cultured on composite aerogels had high ability of adhesion, proliferation and differentiation.
33838188	9	33	theme	application	1342:1352	arg1	potential					1354:1362	application potential	1342:1362	application potential	1342:1362	Totally, the presented biodegradable composite aerogel has application potential in bone tissue engineering.
33838188	8	34	theme	In	1143:1144	arg1	culture					1157:1163	In vitro cell culture	1143:1163	In vitro cell culture	1143:1163	In vitro cell culture showed HEK-293T cells cultured on composite aerogels had high ability of adhesion, proliferation and differentiation.
33838188	6	35	theme	composite	871:879	arg1	material					889:896	the composite aerogel material	867:896	the composite aerogel material	867:896	Through uniaxial compression, the density of the composite aerogel material was close to the one of natural bone, and mechanical strength and toughness were high.
33838188	8	36	theme	proliferation	1248:1260	arg1	ability					1227:1233	high ability	1222:1233	high ability of adhesion, proliferation and differentiation	1222:1280	In vitro cell culture showed HEK-293T cells cultured on composite aerogels had high ability of adhesion, proliferation and differentiation.
33838188	3	37	theme	biomaterials	495:506	arg1	toughness					444:452	toughness	444:452	toughness	444:452	The improvements in toughness and mechanical strength of aerogel-based biomaterials are in great need.
33838188	3	37	theme	biomaterials	495:506	arg1	strength					469:476	mechanical strength	458:476	mechanical strength	458:476	The improvements in toughness and mechanical strength of aerogel-based biomaterials are in great need.
33838188	0	38	theme	bone	17:20	arg1	material					22:29	A new cancellous bone material	0:29	A new cancellous bone material of silk fibroin/cellulose dual network composite aerogel	0:86	A new cancellous bone material of silk fibroin/cellulose dual network composite aerogel reinforced by nano-hydroxyapatite filler.
33838188	5	39	theme	aerosol	722:728	arg1	materials					730:738	the composite aerosol materials	708:738	the composite aerosol materials interlaced by sheet-like SF and reticular cellulose wrapping n-HA on the surface	708:819	A dual network structure was shaped in the composite aerosol materials interlaced by sheet-like SF and reticular cellulose wrapping n-HA on the surface.
33838188	1	40	theme	n-HA	229:232	arg1	fillers					235:241	inorganic nano-hydroxyapatite (n-HA) fillers	198:241	inorganic nano-hydroxyapatite (n-HA) fillers	198:241	Composites materials comprised of biopolymeric aerogel matrices and inorganic nano-hydroxyapatite (n-HA) fillers have received considerable attention in bone engineering.
33838188	0	41	theme	cancellous	6:15	arg1	material					22:29	A new cancellous bone material	0:29	A new cancellous bone material of silk fibroin/cellulose dual network composite aerogel	0:86	A new cancellous bone material of silk fibroin/cellulose dual network composite aerogel reinforced by nano-hydroxyapatite filler.
33838188	7	42	contain	has	1031:1033	arg1	aerogel					1023:1029	the composite aerogel	1009:1029	the composite aerogel	1009:1029	Our work indicates that the composite aerogel has the same mechanical strength range as cancellous bone when the ratio of cellulose, n-HA and SF being 8:1:1.
33838188	7	42	contain	has	1031:1033	arg2	range					1064:1068	the same mechanical strength range	1035:1068	the same mechanical strength range	1035:1068	Our work indicates that the composite aerogel has the same mechanical strength range as cancellous bone when the ratio of cellulose, n-HA and SF being 8:1:1.
33838188	2	43	theme	aerogel-based	339:351	arg1	biomaterials					353:364	aerogel-based biomaterials	339:364	aerogel-based biomaterials	339:364	Although with significant progress in aerogel-based biomaterials, the brittleness and low strengths limit the application.
33838188	4	44	theme	composite	650:658	arg1	aerosol					660:666	composite aerosol	650:666	composite aerosol	650:666	In this work, an alkali urea system was used to dissolve, regenerate and gelate cellulose and silk fibroin (SF) to prepare composite aerosol.
33838188	2	45	theme	low	387:389	arg1	strengths					391:399	the brittleness and low strengths	367:399	the brittleness and low strengths	367:399	Although with significant progress in aerogel-based biomaterials, the brittleness and low strengths limit the application.
33838188	5	46	theme	interlaced	740:749	arg1	materials					730:738	the composite aerosol materials	708:738	the composite aerosol materials interlaced by sheet-like SF and reticular cellulose wrapping n-HA on the surface	708:819	A dual network structure was shaped in the composite aerosol materials interlaced by sheet-like SF and reticular cellulose wrapping n-HA on the surface.
33838188	8	47	theme	adhesion	1238:1245	arg1	ability					1227:1233	high ability	1222:1233	high ability of adhesion, proliferation and differentiation	1222:1280	In vitro cell culture showed HEK-293T cells cultured on composite aerogels had high ability of adhesion, proliferation and differentiation.
33838188	3	48	theme	great	515:519	arg1	need					521:524	great need	515:524	great need	515:524	The improvements in toughness and mechanical strength of aerogel-based biomaterials are in great need.
33838188	8	49	theme	composite	1199:1207	arg1	aerogels					1209:1216	composite aerogels	1199:1216	composite aerogels	1199:1216	In vitro cell culture showed HEK-293T cells cultured on composite aerogels had high ability of adhesion, proliferation and differentiation.
33838188	9	50	theme	tissue	1372:1377	arg1	engineering					1379:1389	bone tissue engineering	1367:1389	bone tissue engineering	1367:1389	Totally, the presented biodegradable composite aerogel has application potential in bone tissue engineering.
33838188	7	51	theme	cancellous	1073:1082	arg1	bone					1084:1087	cancellous bone	1073:1087	cancellous bone when the ratio of cellulose, n-HA and SF	1073:1128	Our work indicates that the composite aerogel has the same mechanical strength range as cancellous bone when the ratio of cellulose, n-HA and SF being 8:1:1.
33838188	6	52	theme	material	889:896	arg1	one					915:917	the one	911:917	the one of natural bone	911:933	Through uniaxial compression, the density of the composite aerogel material was close to the one of natural bone, and mechanical strength and toughness were high.
33838188	6	52	theme	material	889:896	arg1	density					856:862	the density	852:862	the density of the composite aerogel material	852:896	Through uniaxial compression, the density of the composite aerogel material was close to the one of natural bone, and mechanical strength and toughness were high.
33838188	5	53	theme	reticular	772:780	arg1	n-HA					801:804	reticular cellulose wrapping n-HA	772:804	reticular cellulose wrapping n-HA	772:804	A dual network structure was shaped in the composite aerosol materials interlaced by sheet-like SF and reticular cellulose wrapping n-HA on the surface.
33838188	0	54	theme	fibroin/cellulose	39:55	arg1	aerogel					80:86	silk fibroin/cellulose dual network composite aerogel	34:86	silk fibroin/cellulose dual network composite aerogel	34:86	A new cancellous bone material of silk fibroin/cellulose dual network composite aerogel reinforced by nano-hydroxyapatite filler.
33838188	8	55	theme	cell	1152:1155	arg1	culture					1157:1163	In vitro cell culture	1143:1163	In vitro cell culture	1143:1163	In vitro cell culture showed HEK-293T cells cultured on composite aerogels had high ability of adhesion, proliferation and differentiation.
33838188	7	56	theme	strength	1055:1062	arg1	range					1064:1068	the same mechanical strength range	1035:1068	the same mechanical strength range	1035:1068	Our work indicates that the composite aerogel has the same mechanical strength range as cancellous bone when the ratio of cellulose, n-HA and SF being 8:1:1.
33838188	5	57	theme	wrapping	792:799	arg1	n-HA					801:804	reticular cellulose wrapping n-HA	772:804	reticular cellulose wrapping n-HA	772:804	A dual network structure was shaped in the composite aerosol materials interlaced by sheet-like SF and reticular cellulose wrapping n-HA on the surface.
33838188	0	58	theme	silk	34:37	arg1	aerogel					80:86	silk fibroin/cellulose dual network composite aerogel	34:86	silk fibroin/cellulose dual network composite aerogel	34:86	A new cancellous bone material of silk fibroin/cellulose dual network composite aerogel reinforced by nano-hydroxyapatite filler.
33838188	5	59	from	n-HA	801:804	arg1	surface					813:819	the surface	809:819	the surface	809:819	A dual network structure was shaped in the composite aerosol materials interlaced by sheet-like SF and reticular cellulose wrapping n-HA on the surface.
33838188	7	60	theme	cellulose	1107:1115	arg1	ratio					1098:1102	the ratio	1094:1102	the ratio of cellulose, n-HA and SF	1094:1128	Our work indicates that the composite aerogel has the same mechanical strength range as cancellous bone when the ratio of cellulose, n-HA and SF being 8:1:1.
33838188	7	61	theme	composite	1013:1021	arg1	aerogel					1023:1029	the composite aerogel	1009:1029	the composite aerogel	1009:1029	Our work indicates that the composite aerogel has the same mechanical strength range as cancellous bone when the ratio of cellulose, n-HA and SF being 8:1:1.
33838188	1	62	theme	Composites	130:139	arg1	materials					141:149	Composites materials	130:149	Composites materials comprised of biopolymeric aerogel matrices and inorganic nano-hydroxyapatite (n-HA) fillers	130:241	Composites materials comprised of biopolymeric aerogel matrices and inorganic nano-hydroxyapatite (n-HA) fillers have received considerable attention in bone engineering.
33838188	7	63	theme	SF	1127:1128	arg1	ratio					1098:1102	the ratio	1094:1102	the ratio of cellulose, n-HA and SF	1094:1128	Our work indicates that the composite aerogel has the same mechanical strength range as cancellous bone when the ratio of cellulose, n-HA and SF being 8:1:1.
33838188	6	64	theme	mechanical	940:949	arg1	strength					951:958	mechanical strength	940:958	mechanical strength	940:958	Through uniaxial compression, the density of the composite aerogel material was close to the one of natural bone, and mechanical strength and toughness were high.
33838188	7	65	theme	same	1039:1042	arg1	range					1064:1068	the same mechanical strength range	1035:1068	the same mechanical strength range	1035:1068	Our work indicates that the composite aerogel has the same mechanical strength range as cancellous bone when the ratio of cellulose, n-HA and SF being 8:1:1.
33838188	9	66	contain	has	1338:1340	arg1	aerogel					1330:1336	the presented biodegradable composite aerogel	1292:1336	the presented biodegradable composite aerogel	1292:1336	Totally, the presented biodegradable composite aerogel has application potential in bone tissue engineering.
33838188	9	66	contain	has	1338:1340	arg2	potential					1354:1362	application potential	1342:1362	application potential	1342:1362	Totally, the presented biodegradable composite aerogel has application potential in bone tissue engineering.
33838188	5	67	theme	composite	712:720	arg1	materials					730:738	the composite aerosol materials	708:738	the composite aerosol materials interlaced by sheet-like SF and reticular cellulose wrapping n-HA on the surface	708:819	A dual network structure was shaped in the composite aerosol materials interlaced by sheet-like SF and reticular cellulose wrapping n-HA on the surface.
33838188	2	68	theme	brittleness	371:381	arg1	strengths					391:399	the brittleness and low strengths	367:399	the brittleness and low strengths	367:399	Although with significant progress in aerogel-based biomaterials, the brittleness and low strengths limit the application.
33838188	8	69	theme	high	1222:1225	arg1	ability					1227:1233	high ability	1222:1233	high ability of adhesion, proliferation and differentiation	1222:1280	In vitro cell culture showed HEK-293T cells cultured on composite aerogels had high ability of adhesion, proliferation and differentiation.
33838188	1	70	theme	considerable	257:268	arg1	attention					270:278	considerable attention	257:278	considerable attention	257:278	Composites materials comprised of biopolymeric aerogel matrices and inorganic nano-hydroxyapatite (n-HA) fillers have received considerable attention in bone engineering.
33838188	0	71	theme	network	62:68	arg1	aerogel					80:86	silk fibroin/cellulose dual network composite aerogel	34:86	silk fibroin/cellulose dual network composite aerogel	34:86	A new cancellous bone material of silk fibroin/cellulose dual network composite aerogel reinforced by nano-hydroxyapatite filler.
33838188	8	72	theme	differentiation	1266:1280	arg1	ability					1227:1233	high ability	1222:1233	high ability of adhesion, proliferation and differentiation	1222:1280	In vitro cell culture showed HEK-293T cells cultured on composite aerogels had high ability of adhesion, proliferation and differentiation.
33838188	2	73	from	progress	327:334	arg1	biomaterials					353:364	aerogel-based biomaterials	339:364	aerogel-based biomaterials	339:364	Although with significant progress in aerogel-based biomaterials, the brittleness and low strengths limit the application.
33838188	0	74	theme	dual	57:60	arg1	aerogel					80:86	silk fibroin/cellulose dual network composite aerogel	34:86	silk fibroin/cellulose dual network composite aerogel	34:86	A new cancellous bone material of silk fibroin/cellulose dual network composite aerogel reinforced by nano-hydroxyapatite filler.
33838188	4	75	theme	urea	551:554	arg1	system					556:561	an alkali urea system	541:561	an alkali urea system	541:561	In this work, an alkali urea system was used to dissolve, regenerate and gelate cellulose and silk fibroin (SF) to prepare composite aerosol.
33838188	1	76	theme	biopolymeric	164:175	arg1	matrices					185:192	biopolymeric aerogel matrices	164:192	biopolymeric aerogel matrices	164:192	Composites materials comprised of biopolymeric aerogel matrices and inorganic nano-hydroxyapatite (n-HA) fillers have received considerable attention in bone engineering.
32725044	2	0	from	effect	385:390	arg1	secretion					399:407	EPS secretion	395:407	EPS secretion	395:407	It was observed that Tween 40 and ethanol displayed a stimulatory effect on EPS secretion.
32725044	3	1	theme	EPS-C	595:599	arg1	weights					567:573	molecular weights	557:573	molecular weights of EPS-T, EPS-E and EPS-C	557:599	The EPSs obtained by the addition of Tween 40 (EPS-T), ethanol (EPS-E) and control (EPS-C) were purified by Sepharose CL-6B gel chromatography and molecular weights of EPS-T, EPS-E and EPS-C were estimated to be 22.1, 30.0, and 40.5 kDa, respectively.
32725044	1	2	from	rickii	293:298	arg1	secretion					204:212	secretion	204:212	secretion	204:212	In this study, the effects of Tween 40 and ethanol supplementation on the secretion, structure and antioxidant activities of exopolysaccharide (EPS) from Inonotus rickii were investigated.
32725044	1	2	from	rickii	293:298	arg1	structure					215:223	structure	215:223	structure	215:223	In this study, the effects of Tween 40 and ethanol supplementation on the secretion, structure and antioxidant activities of exopolysaccharide (EPS) from Inonotus rickii were investigated.
32725044	1	2	from	rickii	293:298	arg1	activities					241:250	antioxidant activities	229:250	antioxidant activities	229:250	In this study, the effects of Tween 40 and ethanol supplementation on the secretion, structure and antioxidant activities of exopolysaccharide (EPS) from Inonotus rickii were investigated.
32725044	3	3	theme	molecular	557:565	arg1	weights					567:573	molecular weights	557:573	molecular weights of EPS-T, EPS-E and EPS-C	557:599	The EPSs obtained by the addition of Tween 40 (EPS-T), ethanol (EPS-E) and control (EPS-C) were purified by Sepharose CL-6B gel chromatography and molecular weights of EPS-T, EPS-E and EPS-C were estimated to be 22.1, 30.0, and 40.5 kDa, respectively.
32725044	0	4	from	Effect	0:5	arg1	structure					49:57	structure	49:57	structure	49:57	Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.
32725044	0	4	from	Effect	0:5	arg1	activities					75:84	antioxidant activities	63:84	antioxidant activities	63:84	Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.
32725044	0	4	from	Effect	0:5	arg1	secretion					38:46	secretion	38:46	secretion	38:46	Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.
32725044	1	5	from	effects	149:155	arg1	secretion					204:212	secretion	204:212	secretion	204:212	In this study, the effects of Tween 40 and ethanol supplementation on the secretion, structure and antioxidant activities of exopolysaccharide (EPS) from Inonotus rickii were investigated.
32725044	1	5	from	effects	149:155	arg1	structure					215:223	structure	215:223	structure	215:223	In this study, the effects of Tween 40 and ethanol supplementation on the secretion, structure and antioxidant activities of exopolysaccharide (EPS) from Inonotus rickii were investigated.
32725044	1	5	from	effects	149:155	arg1	activities					241:250	antioxidant activities	229:250	antioxidant activities	229:250	In this study, the effects of Tween 40 and ethanol supplementation on the secretion, structure and antioxidant activities of exopolysaccharide (EPS) from Inonotus rickii were investigated.
32725044	1	6	theme	antioxidant	229:239	arg1	activities					241:250	antioxidant activities	229:250	antioxidant activities	229:250	In this study, the effects of Tween 40 and ethanol supplementation on the secretion, structure and antioxidant activities of exopolysaccharide (EPS) from Inonotus rickii were investigated.
32725044	0	7	theme	antioxidant	63:73	arg1	activities					75:84	antioxidant activities	63:84	antioxidant activities	63:84	Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.
32725044	0	8	theme	exopolysaccharides	89:106	arg1	structure					49:57	structure	49:57	structure	49:57	Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.
32725044	0	8	theme	exopolysaccharides	89:106	arg1	activities					75:84	antioxidant activities	63:84	antioxidant activities	63:84	Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.
32725044	0	8	theme	exopolysaccharides	89:106	arg1	secretion					38:46	secretion	38:46	secretion	38:46	Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.
32725044	4	9	theme	Monosaccharide	662:675	arg1	analysis					689:696	Monosaccharide composition analysis	662:696	Monosaccharide composition analysis	662:696	Monosaccharide composition analysis indicated that EPS-T, EPS-E and EPS-C were mainly composed of mannose and glucose.
32725044	3	10	theme	EPS-T	578:582	arg1	weights					567:573	molecular weights	557:573	molecular weights of EPS-T, EPS-E and EPS-C	557:599	The EPSs obtained by the addition of Tween 40 (EPS-T), ethanol (EPS-E) and control (EPS-C) were purified by Sepharose CL-6B gel chromatography and molecular weights of EPS-T, EPS-E and EPS-C were estimated to be 22.1, 30.0, and 40.5 kDa, respectively.
32725044	5	11	theme	DPPH	825:828	arg1	activities					841:850	DPPH scavenging activities	825:850	DPPH scavenging activities	825:850	Furthermore, EPS-E exhibited better OH• and DPPH scavenging activities than those of EPS-C and EPS-T, which might be associated with its molecular characterization.
32725044	5	12	theme	scavenging	830:839	arg1	activities					841:850	DPPH scavenging activities	825:850	DPPH scavenging activities	825:850	Furthermore, EPS-E exhibited better OH• and DPPH scavenging activities than those of EPS-C and EPS-T, which might be associated with its molecular characterization.
32725044	0	13	from	structure	49:57	arg1	rickii					122:127	Inonotus rickii	113:127	Inonotus rickii	113:127	Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.
32725044	1	14	theme	exopolysaccharide	255:271	arg1	secretion					204:212	secretion	204:212	secretion	204:212	In this study, the effects of Tween 40 and ethanol supplementation on the secretion, structure and antioxidant activities of exopolysaccharide (EPS) from Inonotus rickii were investigated.
32725044	1	14	theme	exopolysaccharide	255:271	arg1	structure					215:223	structure	215:223	structure	215:223	In this study, the effects of Tween 40 and ethanol supplementation on the secretion, structure and antioxidant activities of exopolysaccharide (EPS) from Inonotus rickii were investigated.
32725044	1	14	theme	exopolysaccharide	255:271	arg1	activities					241:250	antioxidant activities	229:250	antioxidant activities	229:250	In this study, the effects of Tween 40 and ethanol supplementation on the secretion, structure and antioxidant activities of exopolysaccharide (EPS) from Inonotus rickii were investigated.
32725044	5	15	theme	molecular	918:926	arg1	characterization					928:943	its molecular characterization	914:943	its molecular characterization	914:943	Furthermore, EPS-E exhibited better OH• and DPPH scavenging activities than those of EPS-C and EPS-T, which might be associated with its molecular characterization.
32725044	2	16	theme	EPS	395:397	arg1	secretion					399:407	EPS secretion	395:407	EPS secretion	395:407	It was observed that Tween 40 and ethanol displayed a stimulatory effect on EPS secretion.
32725044	1	17	dep	secretion	204:212	arg1	the					200:202	the	200:202	the	200:202	In this study, the effects of Tween 40 and ethanol supplementation on the secretion, structure and antioxidant activities of exopolysaccharide (EPS) from Inonotus rickii were investigated.
32725044	0	18	dep	secretion	38:46	arg1	the					34:36	the	34:36	the	34:36	Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.
32725044	0	19	theme	ethanol	23:29	arg1	Effect					0:5	Effect	0:5	Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.	0:128	Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.
32725044	1	20	theme	ethanol	173:179	arg1	supplementation					181:195	ethanol supplementation	173:195	ethanol supplementation	173:195	In this study, the effects of Tween 40 and ethanol supplementation on the secretion, structure and antioxidant activities of exopolysaccharide (EPS) from Inonotus rickii were investigated.
32725044	1	21	theme	Tween	160:164	arg1	effects					149:155	the effects	145:155	the effects of Tween 40 and ethanol supplementation on the secretion, structure and antioxidant activities of exopolysaccharide (EPS) from Inonotus rickii	145:298	In this study, the effects of Tween 40 and ethanol supplementation on the secretion, structure and antioxidant activities of exopolysaccharide (EPS) from Inonotus rickii were investigated.
32725044	2	22	theme	stimulatory	373:383	arg1	effect					385:390	a stimulatory effect	371:390	a stimulatory effect on EPS secretion	371:407	It was observed that Tween 40 and ethanol displayed a stimulatory effect on EPS secretion.
32725044	1	23	theme	supplementation	181:195	arg1	effects					149:155	the effects	145:155	the effects of Tween 40 and ethanol supplementation on the secretion, structure and antioxidant activities of exopolysaccharide (EPS) from Inonotus rickii	145:298	In this study, the effects of Tween 40 and ethanol supplementation on the secretion, structure and antioxidant activities of exopolysaccharide (EPS) from Inonotus rickii were investigated.
32725044	0	24	from	secretion	38:46	arg1	rickii					122:127	Inonotus rickii	113:127	Inonotus rickii	113:127	Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.
32725044	3	25	theme	Tween	447:451	arg1	addition					435:442	the addition	431:442	the addition of Tween 40 (EPS-T), ethanol (EPS-E) and control (EPS-C)	431:499	The EPSs obtained by the addition of Tween 40 (EPS-T), ethanol (EPS-E) and control (EPS-C) were purified by Sepharose CL-6B gel chromatography and molecular weights of EPS-T, EPS-E and EPS-C were estimated to be 22.1, 30.0, and 40.5 kDa, respectively.
32725044	3	26	theme	EPS-E	585:589	arg1	weights					567:573	molecular weights	557:573	molecular weights of EPS-T, EPS-E and EPS-C	557:599	The EPSs obtained by the addition of Tween 40 (EPS-T), ethanol (EPS-E) and control (EPS-C) were purified by Sepharose CL-6B gel chromatography and molecular weights of EPS-T, EPS-E and EPS-C were estimated to be 22.1, 30.0, and 40.5 kDa, respectively.
32725044	5	27	theme	better	810:815	arg1	OH•					817:819	better OH•	810:819	better OH•	810:819	Furthermore, EPS-E exhibited better OH• and DPPH scavenging activities than those of EPS-C and EPS-T, which might be associated with its molecular characterization.
32725044	3	28	theme	CL-6B	528:532	arg1	chromatography					538:551	Sepharose CL-6B gel chromatography	518:551	Sepharose CL-6B gel chromatography	518:551	The EPSs obtained by the addition of Tween 40 (EPS-T), ethanol (EPS-E) and control (EPS-C) were purified by Sepharose CL-6B gel chromatography and molecular weights of EPS-T, EPS-E and EPS-C were estimated to be 22.1, 30.0, and 40.5 kDa, respectively.
32725044	3	29	theme	gel	534:536	arg1	chromatography					538:551	Sepharose CL-6B gel chromatography	518:551	Sepharose CL-6B gel chromatography	518:551	The EPSs obtained by the addition of Tween 40 (EPS-T), ethanol (EPS-E) and control (EPS-C) were purified by Sepharose CL-6B gel chromatography and molecular weights of EPS-T, EPS-E and EPS-C were estimated to be 22.1, 30.0, and 40.5 kDa, respectively.
32725044	0	30	from	activities	75:84	arg1	rickii					122:127	Inonotus rickii	113:127	Inonotus rickii	113:127	Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.
32725044	4	31	theme	composition	677:687	arg1	analysis					689:696	Monosaccharide composition analysis	662:696	Monosaccharide composition analysis	662:696	Monosaccharide composition analysis indicated that EPS-T, EPS-E and EPS-C were mainly composed of mannose and glucose.
32725044	0	32	from	rickii	122:127	arg1	structure					49:57	structure	49:57	structure	49:57	Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.
32725044	0	32	from	rickii	122:127	arg1	activities					75:84	antioxidant activities	63:84	antioxidant activities	63:84	Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.
32725044	0	32	from	rickii	122:127	arg1	secretion					38:46	secretion	38:46	secretion	38:46	Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.
32725044	0	32	from	rickii	122:127	arg1	exopolysaccharides					89:106	exopolysaccharides	89:106	exopolysaccharides from Inonotus rickii	89:127	Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.
32725044	3	33	theme	Sepharose	518:526	arg1	chromatography					538:551	Sepharose CL-6B gel chromatography	518:551	Sepharose CL-6B gel chromatography	518:551	The EPSs obtained by the addition of Tween 40 (EPS-T), ethanol (EPS-E) and control (EPS-C) were purified by Sepharose CL-6B gel chromatography and molecular weights of EPS-T, EPS-E and EPS-C were estimated to be 22.1, 30.0, and 40.5 kDa, respectively.
32725044	0	34	theme	tween	10:14	arg1	Effect					0:5	Effect	0:5	Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.	0:128	Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.
34240758	2	0	theme	novel	440:444	arg1	system					467:472	a novel dual electrophoretic system	438:472	a novel dual electrophoretic system based on the combined use of β-CD and ionic liquid (IL) as buffer additives	438:548	The present work deals with the development of a novel dual electrophoretic system based on the combined use of β-CD and ionic liquid (IL) as buffer additives.
34240758	2	1	theme	buffer	533:538	arg1	additives					540:548	buffer additives	533:548	buffer additives	533:548	The present work deals with the development of a novel dual electrophoretic system based on the combined use of β-CD and ionic liquid (IL) as buffer additives.
34240758	5	2	theme	ripening	1219:1226	arg1	stage					1228:1232	the ripening stage	1215:1232	the ripening stage	1215:1232	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	1	3	theme	fast	207:210	arg1	method					238:243	a simple, reliable, and fast capillary electrophoretic method	183:243	a simple, reliable, and fast capillary electrophoretic method	183:243	In this work, a simple, reliable, and fast capillary electrophoretic method was developed and validated for the simultaneous determination of 12 polyphenolic compounds, the most frequently found in carob's pulp and seeds.
34240758	5	4	theme	polyphenolic	1244:1255	arg1	composition					1257:1267	carobs polyphenolic composition	1237:1267	carobs polyphenolic composition	1237:1267	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	1	5	theme	polyphenolic	314:325	arg1	compounds					327:335	12 polyphenolic compounds	311:335	12 polyphenolic compounds	311:335	In this work, a simple, reliable, and fast capillary electrophoretic method was developed and validated for the simultaneous determination of 12 polyphenolic compounds, the most frequently found in carob's pulp and seeds.
34240758	0	6	theme	effect	161:166	arg1	study					136:140	carob's phenolics-A study	116:140	carob's phenolics-A study of the synergistic effect	116:166	Combined use of β-cyclodextrin and ionic liquid as electrolyte additives in EKC for separation and determination of carob's phenolics-A study of the synergistic effect.
34240758	5	7	dep	polyphenols	1310:1320	arg1	phenolics					1329:1337	total phenolics	1323:1337	total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp	1323:1397	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	5	8	dep	ripe	1099:1102	arg1	pulp					1123:1126	183.92 μg/g carob pulp	1105:1126	183.92 μg/g carob pulp	1105:1126	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	3	9	theme	buffer	757:762	arg1	additives					764:772	buffer additives	757:772	buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV	757:838	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	3	9	theme	buffer	757:762	arg1	BGE					640:642	a BGE	638:642	a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL	638:752	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	1	10	theme	capillary	212:220	arg1	method					238:243	a simple, reliable, and fast capillary electrophoretic method	183:243	a simple, reliable, and fast capillary electrophoretic method	183:243	In this work, a simple, reliable, and fast capillary electrophoretic method was developed and validated for the simultaneous determination of 12 polyphenolic compounds, the most frequently found in carob's pulp and seeds.
34240758	1	11	theme	compounds	327:335	arg1	determination					294:306	the simultaneous determination	277:306	the simultaneous determination of 12 polyphenolic compounds, the most frequently found in carob's pulp and seeds	277:388	In this work, a simple, reliable, and fast capillary electrophoretic method was developed and validated for the simultaneous determination of 12 polyphenolic compounds, the most frequently found in carob's pulp and seeds.
34240758	4	12	dep	identification	925:938	arg1	the					921:923	the	921:923	the	921:923	The application of the developed electrophoretic method to real samples enabled the identification and quantification of the main phenolic constituents of both ripe and unripe carob pulp extracts.
34240758	1	13	theme	electrophoretic	222:236	arg1	method					238:243	a simple, reliable, and fast capillary electrophoretic method	183:243	a simple, reliable, and fast capillary electrophoretic method	183:243	In this work, a simple, reliable, and fast capillary electrophoretic method was developed and validated for the simultaneous determination of 12 polyphenolic compounds, the most frequently found in carob's pulp and seeds.
34240758	5	14	theme	183.92 μg/g	1105:1115	arg1	pulp					1123:1126	183.92 μg/g carob pulp	1105:1126	183.92 μg/g carob pulp	1105:1126	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	5	15	dep	unripe	1133:1138	arg1	pulp					1159:1162	205.10 μg/g carob pulp	1141:1162	205.10 μg/g carob pulp	1141:1162	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	3	16	theme	lactate	729:735	arg1	IL					751:752	3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL	697:752	3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL	697:752	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	4	17	theme	unripe	1010:1015	arg1	extracts					1028:1035	both ripe and unripe carob pulp extracts	996:1035	both ripe and unripe carob pulp extracts	996:1035	The application of the developed electrophoretic method to real samples enabled the identification and quantification of the main phenolic constituents of both ripe and unripe carob pulp extracts.
34240758	5	18	attach	predominance	1063:1074	arg1	pulp					1171:1174	both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp	1094:1174	both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp	1094:1174	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	5	18	attach	predominance	1063:1074	arg2	acid					1086:1089	gallic acid	1079:1089	gallic acid	1079:1089	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	4	19	theme	ripe	1001:1004	arg1	extracts					1028:1035	both ripe and unripe carob pulp extracts	996:1035	both ripe and unripe carob pulp extracts	996:1035	The application of the developed electrophoretic method to real samples enabled the identification and quantification of the main phenolic constituents of both ripe and unripe carob pulp extracts.
34240758	3	20	theme	ester	723:727	arg1	lactate					729:735	tert butyl ester lactate	712:735	3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL	697:752	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	3	20	theme	ester	723:727	arg1	Lac					746:748	l-AlaC4 Lac	738:748	l-AlaC4 Lac	738:748	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	2	21	theme	system	467:472	arg1	development					423:433	the development	419:433	the development of a novel dual electrophoretic system based on the combined use of β-CD and ionic liquid (IL) as buffer additives	419:548	The present work deals with the development of a novel dual electrophoretic system based on the combined use of β-CD and ionic liquid (IL) as buffer additives.
34240758	5	22	theme	ripe	1099:1102	arg1	pulp					1171:1174	both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp	1094:1174	both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp	1094:1174	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	3	23	theme	butyl	717:721	arg1	lactate					729:735	tert butyl ester lactate	712:735	3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL	697:752	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	3	23	theme	butyl	717:721	arg1	Lac					746:748	l-AlaC4 Lac	738:748	l-AlaC4 Lac	738:748	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	4	24	theme	carob	1017:1021	arg1	extracts					1028:1035	both ripe and unripe carob pulp extracts	996:1035	both ripe and unripe carob pulp extracts	996:1035	The application of the developed electrophoretic method to real samples enabled the identification and quantification of the main phenolic constituents of both ripe and unripe carob pulp extracts.
34240758	5	25	from	polyphenols	1310:1320	arg1	enriched					1298:1305	enriched	1298:1305	enriched	1298:1305	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	5	26	theme	great	1196:1200	arg1	influence					1202:1210	the great influence	1192:1210	the great influence of the ripening stage on carobs polyphenolic composition	1192:1267	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	0	27	theme	study	136:140	arg1	separation					84:93	separation	84:93	separation	84:93	Combined use of β-cyclodextrin and ionic liquid as electrolyte additives in EKC for separation and determination of carob's phenolics-A study of the synergistic effect.
34240758	0	27	theme	study	136:140	arg1	determination					99:111	determination	99:111	determination of carob's phenolics-A study of the synergistic effect	99:166	Combined use of β-cyclodextrin and ionic liquid as electrolyte additives in EKC for separation and determination of carob's phenolics-A study of the synergistic effect.
34240758	5	28	from	influence	1202:1210	arg1	composition					1257:1267	carobs polyphenolic composition	1237:1267	carobs polyphenolic composition	1237:1267	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	0	29	theme	Combined	0:7	arg1	use					9:11	Combined use	0:11	Combined use of β-cyclodextrin and ionic liquid as electrolyte	0:61	Combined use of β-cyclodextrin and ionic liquid as electrolyte additives in EKC for separation and determination of carob's phenolics-A study of the synergistic effect.
34240758	5	30	theme	total	1323:1327	arg1	phenolics					1329:1337	total phenolics	1323:1337	total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp	1323:1397	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	4	31	theme	electrophoretic	874:888	arg1	method					890:895	the developed electrophoretic method	860:895	the developed electrophoretic method	860:895	The application of the developed electrophoretic method to real samples enabled the identification and quantification of the main phenolic constituents of both ripe and unripe carob pulp extracts.
34240758	4	32	theme	method	890:895	arg1	application					845:855	The application	841:855	The application of the developed electrophoretic method to real samples	841:911	The application of the developed electrophoretic method to real samples enabled the identification and quantification of the main phenolic constituents of both ripe and unripe carob pulp extracts.
34240758	4	33	theme	constituents	980:991	arg1	identification					925:938	identification	925:938	identification	925:938	The application of the developed electrophoretic method to real samples enabled the identification and quantification of the main phenolic constituents of both ripe and unripe carob pulp extracts.
34240758	4	33	theme	constituents	980:991	arg1	quantification					944:957	quantification	944:957	quantification	944:957	The application of the developed electrophoretic method to real samples enabled the identification and quantification of the main phenolic constituents of both ripe and unripe carob pulp extracts.
34240758	0	34	theme	β-cyclodextrin	16:29	arg1	use					9:11	Combined use	0:11	Combined use of β-cyclodextrin and ionic liquid as electrolyte	0:61	Combined use of β-cyclodextrin and ionic liquid as electrolyte additives in EKC for separation and determination of carob's phenolics-A study of the synergistic effect.
34240758	2	35	theme	β-CD	503:506	arg1	use					496:498	the combined use	483:498	the combined use of β-CD and ionic liquid (IL) as buffer additives	483:548	The present work deals with the development of a novel dual electrophoretic system based on the combined use of β-CD and ionic liquid (IL) as buffer additives.
34240758	5	36	theme	unripe	1275:1280	arg1	pods					1282:1285	unripe pods	1275:1285	unripe pods	1275:1285	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	4	37	theme	phenolic	971:978	arg1	constituents					980:991	the main phenolic constituents	962:991	the main phenolic constituents of both ripe and unripe carob pulp extracts	962:1035	The application of the developed electrophoretic method to real samples enabled the identification and quantification of the main phenolic constituents of both ripe and unripe carob pulp extracts.
34240758	4	38	theme	real	900:903	arg1	samples					905:911	real samples	900:911	real samples	900:911	The application of the developed electrophoretic method to real samples enabled the identification and quantification of the main phenolic constituents of both ripe and unripe carob pulp extracts.
34240758	5	39	theme	carob	1388:1392	arg1	pulp					1394:1397	283.13 μg/g unripe and ripe carob pulp	1360:1397	pulp	1394:1397	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	5	40	theme	carob	1165:1169	arg1	pulp					1171:1174	both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp	1094:1174	both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp	1094:1174	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	5	41	theme	ripe	1383:1386	arg1	pulp					1394:1397	283.13 μg/g unripe and ripe carob pulp	1360:1397	pulp	1394:1397	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	0	42	theme	ionic	35:39	arg1	electrolyte					51:61	ionic liquid as electrolyte	35:61	ionic liquid as electrolyte	35:61	Combined use of β-cyclodextrin and ionic liquid as electrolyte additives in EKC for separation and determination of carob's phenolics-A study of the synergistic effect.
34240758	3	43	theme	35 mM	658:662	arg1	borate					664:669	35 mM borate	658:669	35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL	658:752	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	0	44	theme	phenolics-A	124:134	arg1	study					136:140	carob's phenolics-A study	116:140	carob's phenolics-A study of the synergistic effect	116:166	Combined use of β-cyclodextrin and ionic liquid as electrolyte additives in EKC for separation and determination of carob's phenolics-A study of the synergistic effect.
34240758	3	45	theme	target	580:585	arg1	analytes					587:594	the target analytes	576:594	the target analytes	576:594	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	5	46	dep	phenolics	1329:1337	arg1	unripe					1372:1377	unripe	1372:1377	unripe	1372:1377	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	5	46	dep	phenolics	1329:1337	arg1	912.58					1349:1354	912.58	1349:1354	912.58	1349:1354	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	3	47	theme	25°C	802:805	arg1	voltage					823:829	an applied voltage	812:829	an applied voltage of 30 kV	812:838	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	3	47	theme	25°C	802:805	arg1	temperature					787:797	a temperature	785:797	a temperature of 25°C	785:805	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	3	47	theme	25°C	802:805	arg1	pH					777:778	pH 9.5	777:782	pH 9.5	777:782	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	3	48	from	voltage	823:829	arg1	additives					764:772	buffer additives	757:772	buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV	757:838	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	3	48	from	voltage	823:829	arg1	BGE					640:642	a BGE	638:642	a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL	638:752	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	4	49	theme	developed	864:872	arg1	method					890:895	the developed electrophoretic method	860:895	the developed electrophoretic method	860:895	The application of the developed electrophoretic method to real samples enabled the identification and quantification of the main phenolic constituents of both ripe and unripe carob pulp extracts.
34240758	2	50	theme	combined	487:494	arg1	use					496:498	the combined use	483:498	the combined use of β-CD and ionic liquid (IL) as buffer additives	483:548	The present work deals with the development of a novel dual electrophoretic system based on the combined use of β-CD and ionic liquid (IL) as buffer additives.
34240758	5	51	theme	gallic	1079:1084	arg1	acid					1086:1089	gallic acid	1079:1089	gallic acid	1079:1089	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	2	52	theme	present	395:401	arg1	work					403:406	The present work	391:406	The present work	391:406	The present work deals with the development of a novel dual electrophoretic system based on the combined use of β-CD and ionic liquid (IL) as buffer additives.
34240758	5	53	theme	stage	1228:1232	arg1	influence					1202:1210	the great influence	1192:1210	the great influence of the ripening stage on carobs polyphenolic composition	1192:1267	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	1	54	theme	simple	185:190	arg1	method					238:243	a simple, reliable, and fast capillary electrophoretic method	183:243	a simple, reliable, and fast capillary electrophoretic method	183:243	In this work, a simple, reliable, and fast capillary electrophoretic method was developed and validated for the simultaneous determination of 12 polyphenolic compounds, the most frequently found in carob's pulp and seeds.
34240758	0	55	theme	as	48:49	arg1	electrolyte					51:61	ionic liquid as electrolyte	35:61	ionic liquid as electrolyte	35:61	Combined use of β-cyclodextrin and ionic liquid as electrolyte additives in EKC for separation and determination of carob's phenolics-A study of the synergistic effect.
34240758	2	56	theme	dual	446:449	arg1	system					467:472	a novel dual electrophoretic system	438:472	a novel dual electrophoretic system based on the combined use of β-CD and ionic liquid (IL) as buffer additives	438:548	The present work deals with the development of a novel dual electrophoretic system based on the combined use of β-CD and ionic liquid (IL) as buffer additives.
34240758	0	57	theme	liquid	41:46	arg1	electrolyte					51:61	ionic liquid as electrolyte	35:61	ionic liquid as electrolyte	35:61	Combined use of β-cyclodextrin and ionic liquid as electrolyte additives in EKC for separation and determination of carob's phenolics-A study of the synergistic effect.
34240758	1	58	theme	simultaneous	281:292	arg1	determination					294:306	the simultaneous determination	277:306	the simultaneous determination of 12 polyphenolic compounds, the most frequently found in carob's pulp and seeds	277:388	In this work, a simple, reliable, and fast capillary electrophoretic method was developed and validated for the simultaneous determination of 12 polyphenolic compounds, the most frequently found in carob's pulp and seeds.
34240758	2	59	theme	liquid	518:523	arg1	use					496:498	the combined use	483:498	the combined use of β-CD and ionic liquid (IL) as buffer additives	483:548	The present work deals with the development of a novel dual electrophoretic system based on the combined use of β-CD and ionic liquid (IL) as buffer additives.
34240758	5	60	from	predominance	1063:1074	arg1	pulp					1171:1174	both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp	1094:1174	both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp	1094:1174	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	5	61	theme	carobs	1237:1242	arg1	composition					1257:1267	carobs polyphenolic composition	1237:1267	carobs polyphenolic composition	1237:1267	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	5	62	theme	unripe	1133:1138	arg1	pulp					1171:1174	both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp	1094:1174	both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp	1094:1174	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	3	63	theme	15 mM	682:686	arg1	β-CD					688:691	15 mM β-CD	682:691	15 mM β-CD	682:691	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	0	64	theme	synergistic	149:159	arg1	effect					161:166	the synergistic effect	145:166	the synergistic effect	145:166	Combined use of β-cyclodextrin and ionic liquid as electrolyte additives in EKC for separation and determination of carob's phenolics-A study of the synergistic effect.
34240758	5	65	theme	205.10 μg/g	1141:1151	arg1	pulp					1159:1162	205.10 μg/g carob pulp	1141:1162	205.10 μg/g carob pulp	1141:1162	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	5	66	theme	acid	1086:1089	arg1	predominance					1063:1074	the predominance	1059:1074	the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp	1059:1174	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	3	67	theme	applied	815:821	arg1	voltage					823:829	an applied voltage	812:829	an applied voltage of 30 kV	812:838	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	3	68	theme	tert	712:715	arg1	lactate					729:735	tert butyl ester lactate	712:735	3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL	697:752	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	3	68	theme	tert	712:715	arg1	Lac					746:748	l-AlaC4 Lac	738:748	l-AlaC4 Lac	738:748	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	3	69	from	pH	777:778	arg1	additives					764:772	buffer additives	757:772	buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV	757:838	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	3	69	from	pH	777:778	arg1	BGE					640:642	a BGE	638:642	a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL	638:752	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	3	70	theme	analytes	587:594	arg1	separation					562:571	A baseline separation	551:571	A baseline separation of the target analytes	551:594	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	3	71	theme	baseline	553:560	arg1	separation					562:571	A baseline separation	551:571	A baseline separation of the target analytes	551:594	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	4	72	theme	extracts	1028:1035	arg1	constituents					980:991	the main phenolic constituents	962:991	the main phenolic constituents of both ripe and unripe carob pulp extracts	962:1035	The application of the developed electrophoretic method to real samples enabled the identification and quantification of the main phenolic constituents of both ripe and unripe carob pulp extracts.
34240758	4	73	theme	main	966:969	arg1	constituents					980:991	the main phenolic constituents	962:991	the main phenolic constituents of both ripe and unripe carob pulp extracts	962:1035	The application of the developed electrophoretic method to real samples enabled the identification and quantification of the main phenolic constituents of both ripe and unripe carob pulp extracts.
34240758	1	74	theme	reliable	193:200	arg1	method					238:243	a simple, reliable, and fast capillary electrophoretic method	183:243	a simple, reliable, and fast capillary electrophoretic method	183:243	In this work, a simple, reliable, and fast capillary electrophoretic method was developed and validated for the simultaneous determination of 12 polyphenolic compounds, the most frequently found in carob's pulp and seeds.
34240758	2	75	theme	electrophoretic	451:465	arg1	system					467:472	a novel dual electrophoretic system	438:472	a novel dual electrophoretic system based on the combined use of β-CD and ionic liquid (IL) as buffer additives	438:548	The present work deals with the development of a novel dual electrophoretic system based on the combined use of β-CD and ionic liquid (IL) as buffer additives.
34240758	4	76	theme	pulp	1023:1026	arg1	extracts					1028:1035	both ripe and unripe carob pulp extracts	996:1035	both ripe and unripe carob pulp extracts	996:1035	The application of the developed electrophoretic method to real samples enabled the identification and quantification of the main phenolic constituents of both ripe and unripe carob pulp extracts.
34240758	5	77	theme	carob	1153:1157	arg1	pulp					1159:1162	205.10 μg/g carob pulp	1141:1162	205.10 μg/g carob pulp	1141:1162	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	3	78	theme	3 mM	697:700	arg1	l-alanine					702:710	l-alanine	702:710	l-alanine	702:710	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	0	79	theme	electrolyte	51:61	arg1	use					9:11	Combined use	0:11	Combined use of β-cyclodextrin and ionic liquid as electrolyte	0:61	Combined use of β-cyclodextrin and ionic liquid as electrolyte additives in EKC for separation and determination of carob's phenolics-A study of the synergistic effect.
34240758	3	80	from	temperature	787:797	arg1	additives					764:772	buffer additives	757:772	buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV	757:838	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	3	80	from	temperature	787:797	arg1	BGE					640:642	a BGE	638:642	a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL	638:752	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	3	81	theme	30 kV	834:838	arg1	voltage					823:829	an applied voltage	812:829	an applied voltage of 30 kV	812:838	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	3	81	theme	30 kV	834:838	arg1	temperature					787:797	a temperature	785:797	a temperature of 25°C	785:805	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	3	81	theme	30 kV	834:838	arg1	pH					777:778	pH 9.5	777:782	pH 9.5	777:782	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
34240758	5	82	from	enriched	1298:1305	arg1	polyphenols					1310:1320	polyphenols	1310:1320	polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp)	1310:1398	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	5	83	theme	carob	1117:1121	arg1	pulp					1123:1126	183.92 μg/g carob pulp	1105:1126	183.92 μg/g carob pulp	1105:1126	The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).
34240758	3	84	theme	l-alanine	702:710	arg1	IL					751:752	3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL	697:752	3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL	697:752	A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC4 Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV.
33049885	2	0	theme	combustion	477:486	arg1	PCFC					506:509	PCFC	506:509	PCFC	506:509	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	2	0	theme	combustion	477:486	arg1	calorimetry					493:503	pyrolysis combustion flow calorimetry	467:503	pyrolysis combustion flow calorimetry (PCFC)	467:510	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	6	1	dep	formation	1325:1333	arg1	c					1313:1313	c	1313:1313	c	1313:1313	A multiplicity of factors can explain this behavior, such as: (a) negligible heat conduction, (b) low heat of combustion, (c) charring formation, and (d) water release.
33049885	2	2	theme	enhanced	350:357	arg1	properties					359:368	promising enhanced properties	340:368	promising enhanced properties for flame retardancy	340:389	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	3	3	theme	heat	797:800	arg1	flux					802:805	heat flux	797:805	heat flux not exceeding 50 kW m-2	797:829	All these methods confirm the promising properties of this alternative material in terms of fire protection (pHRR, THR, EHC, time-to-ignition, flame duration or production of residue), especially for heat flux not exceeding 50 kW m-2.
33049885	2	4	theme	pyrolysis	467:475	arg1	PCFC					506:509	PCFC	506:509	PCFC	506:509	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	2	4	theme	pyrolysis	467:475	arg1	calorimetry					493:503	pyrolysis combustion flow calorimetry	467:503	pyrolysis combustion flow calorimetry (PCFC)	467:510	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	5	5	theme	thickness	997:1005	arg1	effect					984:989	the effect	980:989	the effect of AF thickness	980:1005	The investigation of the effect of AF thickness shows that the critical thickness (CT) is close to 1.5-1.7 cm: heat diffusion and material combustion are limited to the CT layer that protects the underlying layers from combustion.
33049885	2	6	theme	large-scale	576:586	arg1	samples					588:594	large-scale samples	576:594	large-scale samples	576:594	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	5	7	theme	underlying	1155:1164	arg1	layers					1166:1171	the underlying layers	1151:1171	the underlying layers	1151:1171	The investigation of the effect of AF thickness shows that the critical thickness (CT) is close to 1.5-1.7 cm: heat diffusion and material combustion are limited to the CT layer that protects the underlying layers from combustion.
33049885	7	8	theme	underlying	1385:1394	arg1	layers					1396:1401	underlying layers	1385:1401	underlying layers	1385:1401	Water being released from underlying layers, dilutes the gases emitted during the combustion of superficial layers and promotes the flame extinction.
33049885	4	9	theme	retardant	877:885	arg1	properties					887:896	flame retardant properties	871:896	flame retardant properties	871:896	At higher heat flux (i.e., 75 kW m-2), flame retardant properties tend to decrease but maintain at a higher level than FR-PUF.
33049885	3	10	theme	fire	689:692	arg1	protection					694:703	fire protection	689:703	fire protection (pHRR, THR, EHC, time-to-ignition, flame duration or production of residue)	689:779	All these methods confirm the promising properties of this alternative material in terms of fire protection (pHRR, THR, EHC, time-to-ignition, flame duration or production of residue), especially for heat flux not exceeding 50 kW m-2.
33049885	2	11	theme	promising	340:348	arg1	properties					359:368	promising enhanced properties	340:368	promising enhanced properties for flame retardancy	340:389	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	1	12	theme	new	46:48	arg1	foam					71:74	A new biosourced composite foam	44:74	A new biosourced composite foam (AF, associating foamed alginate matrix and orange peel filler)	44:138	A new biosourced composite foam (AF, associating foamed alginate matrix and orange peel filler) is successfully tested for fire-retardant properties.
33049885	6	13	theme	combustion	1300:1309	arg1	release					1350:1356	(d) water release	1340:1356	(d) water release	1340:1356	A multiplicity of factors can explain this behavior, such as: (a) negligible heat conduction, (b) low heat of combustion, (c) charring formation, and (d) water release.
33049885	6	13	theme	combustion	1300:1309	arg1	conduction					1272:1281	negligible heat conduction	1256:1281	: (a) negligible heat conduction	1250:1281	A multiplicity of factors can explain this behavior, such as: (a) negligible heat conduction, (b) low heat of combustion, (c) charring formation, and (d) water release.
33049885	6	13	theme	combustion	1300:1309	arg1	heat					1292:1295	(b) low heat of combustion	1284:1309	(b) low heat of combustion	1284:1309	A multiplicity of factors can explain this behavior, such as: (a) negligible heat conduction, (b) low heat of combustion, (c) charring formation, and (d) water release.
33049885	6	13	theme	combustion	1300:1309	arg1	formation					1325:1333	(c) charring formation	1312:1333	(c) charring formation	1312:1333	A multiplicity of factors can explain this behavior, such as: (a) negligible heat conduction, (b) low heat of combustion, (c) charring formation, and (d) water release.
33049885	4	14	theme	flame	871:875	arg1	properties					887:896	flame retardant properties	871:896	flame retardant properties	871:896	At higher heat flux (i.e., 75 kW m-2), flame retardant properties tend to decrease but maintain at a higher level than FR-PUF.
33049885	2	15	theme	polyurethane	285:296	arg1	FR-PUF					304:309	FR-PUF	304:309	FR-PUF	304:309	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	2	15	theme	polyurethane	285:296	arg1	foam					298:301	fire-retardant polyurethane foam	270:301	fire-retardant polyurethane foam (FR-PUF, a commercial product)	270:332	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	1	16	theme	biosourced	50:59	arg1	foam					71:74	A new biosourced composite foam	44:74	A new biosourced composite foam (AF, associating foamed alginate matrix and orange peel filler)	44:138	A new biosourced composite foam (AF, associating foamed alginate matrix and orange peel filler) is successfully tested for fire-retardant properties.
33049885	6	17	dep	conduction	1272:1281	arg1	a					1253:1253	a	1253:1253	a	1253:1253	A multiplicity of factors can explain this behavior, such as: (a) negligible heat conduction, (b) low heat of combustion, (c) charring formation, and (d) water release.
33049885	4	18	dep	m-2	865:867	arg1	i.e.					853:856	i.e.	853:856	i.e.	853:856	At higher heat flux (i.e., 75 kW m-2), flame retardant properties tend to decrease but maintain at a higher level than FR-PUF.
33049885	2	19	dep	FR-PUF	304:309	arg1	product					325:331	a commercial product	312:331	a commercial product	312:331	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	1	20	theme	composite	61:69	arg1	foam					71:74	A new biosourced composite foam	44:74	A new biosourced composite foam (AF, associating foamed alginate matrix and orange peel filler)	44:138	A new biosourced composite foam (AF, associating foamed alginate matrix and orange peel filler) is successfully tested for fire-retardant properties.
33049885	6	21	theme	charring	1316:1323	arg1	formation					1325:1333	(c) charring formation	1312:1333	(c) charring formation	1312:1333	A multiplicity of factors can explain this behavior, such as: (a) negligible heat conduction, (b) low heat of combustion, (c) charring formation, and (d) water release.
33049885	4	22	theme	75 kW	859:863	arg1	m-2					865:867	75 kW m-2	859:867	75 kW m-2	859:867	At higher heat flux (i.e., 75 kW m-2), flame retardant properties tend to decrease but maintain at a higher level than FR-PUF.
33049885	4	22	theme	75 kW	859:863	arg1	flux					847:850	higher heat flux	835:850	higher heat flux (i.e., 75 kW m-2)	835:868	At higher heat flux (i.e., 75 kW m-2), flame retardant properties tend to decrease but maintain at a higher level than FR-PUF.
33049885	5	23	theme	critical	1022:1029	arg1	1.5-1.7 cm					1058:1067	1.5-1.7 cm	1058:1067	1.5-1.7 cm	1058:1067	The investigation of the effect of AF thickness shows that the critical thickness (CT) is close to 1.5-1.7 cm: heat diffusion and material combustion are limited to the CT layer that protects the underlying layers from combustion.
33049885	5	23	theme	critical	1022:1029	arg1	CT					1042:1043	CT	1042:1043	CT	1042:1043	The investigation of the effect of AF thickness shows that the critical thickness (CT) is close to 1.5-1.7 cm: heat diffusion and material combustion are limited to the CT layer that protects the underlying layers from combustion.
33049885	5	23	theme	critical	1022:1029	arg1	thickness					1031:1039	the critical thickness	1018:1039	the critical thickness (CT)	1018:1044	The investigation of the effect of AF thickness shows that the critical thickness (CT) is close to 1.5-1.7 cm: heat diffusion and material combustion are limited to the CT layer that protects the underlying layers from combustion.
33049885	6	24	dep	release	1350:1356	arg1	d					1341:1341	d	1341:1341	d	1341:1341	A multiplicity of factors can explain this behavior, such as: (a) negligible heat conduction, (b) low heat of combustion, (c) charring formation, and (d) water release.
33049885	0	25	theme	Fire	0:3	arg1	behavior					5:12	Fire behavior	0:12	Fire behavior of innovative alginate	0:35	Fire behavior of innovative alginate foams.
33049885	5	26	theme	material	1089:1096	arg1	combustion					1098:1107	material combustion	1089:1107	material combustion	1089:1107	The investigation of the effect of AF thickness shows that the critical thickness (CT) is close to 1.5-1.7 cm: heat diffusion and material combustion are limited to the CT layer that protects the underlying layers from combustion.
33049885	7	27	theme	superficial	1455:1465	arg1	layers					1467:1472	superficial layers	1455:1472	superficial layers	1455:1472	Water being released from underlying layers, dilutes the gases emitted during the combustion of superficial layers and promotes the flame extinction.
33049885	6	28	theme	water	1344:1348	arg1	release					1350:1356	(d) water release	1340:1356	(d) water release	1340:1356	A multiplicity of factors can explain this behavior, such as: (a) negligible heat conduction, (b) low heat of combustion, (c) charring formation, and (d) water release.
33049885	2	29	theme	thermogravimetric	433:449	arg1	TGA					461:463	TGA	461:463	TGA	461:463	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	2	29	theme	thermogravimetric	433:449	arg1	analysis					451:458	thermogravimetric analysis	433:458	thermogravimetric analysis (TGA)	433:464	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	5	30	theme	heat	1070:1073	arg1	diffusion					1075:1083	heat diffusion	1070:1083	heat diffusion	1070:1083	The investigation of the effect of AF thickness shows that the critical thickness (CT) is close to 1.5-1.7 cm: heat diffusion and material combustion are limited to the CT layer that protects the underlying layers from combustion.
33049885	5	31	theme	effect	984:989	arg1	investigation					963:975	The investigation	959:975	The investigation of the effect of AF thickness	959:1005	The investigation of the effect of AF thickness shows that the critical thickness (CT) is close to 1.5-1.7 cm: heat diffusion and material combustion are limited to the CT layer that protects the underlying layers from combustion.
33049885	2	32	theme	flame	374:378	arg1	retardancy					380:389	flame retardancy	374:389	flame retardancy	374:389	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	0	33	theme	innovative	17:26	arg1	alginate					28:35	innovative alginate	17:35	innovative alginate	17:35	Fire behavior of innovative alginate foams.
33049885	7	34	theme	layers	1467:1472	arg1	combustion					1441:1450	the combustion	1437:1450	the combustion of superficial layers	1437:1472	Water being released from underlying layers, dilutes the gases emitted during the combustion of superficial layers and promotes the flame extinction.
33049885	7	35	theme	flame	1491:1495	arg1	extinction					1497:1506	the flame extinction	1487:1506	the flame extinction	1487:1506	Water being released from underlying layers, dilutes the gases emitted during the combustion of superficial layers and promotes the flame extinction.
33049885	1	36	theme	fire-retardant	167:180	arg1	properties					182:191	fire-retardant properties	167:191	fire-retardant properties	167:191	A new biosourced composite foam (AF, associating foamed alginate matrix and orange peel filler) is successfully tested for fire-retardant properties.
33049885	3	37	dep	protection	694:703	arg1	THR					712:714	THR	712:714	THR	712:714	All these methods confirm the promising properties of this alternative material in terms of fire protection (pHRR, THR, EHC, time-to-ignition, flame duration or production of residue), especially for heat flux not exceeding 50 kW m-2.
33049885	3	37	dep	protection	694:703	arg1	duration					746:753	flame duration	740:753	flame duration	740:753	All these methods confirm the promising properties of this alternative material in terms of fire protection (pHRR, THR, EHC, time-to-ignition, flame duration or production of residue), especially for heat flux not exceeding 50 kW m-2.
33049885	3	37	dep	protection	694:703	arg1	production					758:767	production	758:767	production of residue	758:778	All these methods confirm the promising properties of this alternative material in terms of fire protection (pHRR, THR, EHC, time-to-ignition, flame duration or production of residue), especially for heat flux not exceeding 50 kW m-2.
33049885	3	37	dep	protection	694:703	arg1	EHC					717:719	EHC	717:719	EHC	717:719	All these methods confirm the promising properties of this alternative material in terms of fire protection (pHRR, THR, EHC, time-to-ignition, flame duration or production of residue), especially for heat flux not exceeding 50 kW m-2.
33049885	3	37	dep	protection	694:703	arg1	time-to-ignition					722:737	time-to-ignition	722:737	time-to-ignition	722:737	All these methods confirm the promising properties of this alternative material in terms of fire protection (pHRR, THR, EHC, time-to-ignition, flame duration or production of residue), especially for heat flux not exceeding 50 kW m-2.
33049885	3	37	dep	protection	694:703	arg1	pHRR					706:709	pHRR	706:709	pHRR	706:709	All these methods confirm the promising properties of this alternative material in terms of fire protection (pHRR, THR, EHC, time-to-ignition, flame duration or production of residue), especially for heat flux not exceeding 50 kW m-2.
33049885	2	38	theme	designed	524:531	arg1	apparatus					533:541	a newly designed apparatus	516:541	a newly designed apparatus called RAPACES for investigating large-scale samples	516:594	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	1	39	theme	associating	81:91	arg1	matrix					109:114	associating foamed alginate matrix	81:114	associating foamed alginate matrix	81:114	A new biosourced composite foam (AF, associating foamed alginate matrix and orange peel filler) is successfully tested for fire-retardant properties.
33049885	2	40	theme	different	407:415	arg1	calorimetry					493:503	pyrolysis combustion flow calorimetry	467:503	pyrolysis combustion flow calorimetry (PCFC)	467:510	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	2	40	theme	different	407:415	arg1	apparatus					533:541	a newly designed apparatus	516:541	a newly designed apparatus called RAPACES for investigating large-scale samples	516:594	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	2	40	theme	different	407:415	arg1	analysis					451:458	thermogravimetric analysis	433:458	thermogravimetric analysis (TGA)	433:464	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	2	40	theme	different	407:415	arg1	methods					417:423	different methods	407:423	different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples	407:594	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	1	41	dep	foam	71:74	arg1	matrix					109:114	associating foamed alginate matrix	81:114	associating foamed alginate matrix	81:114	A new biosourced composite foam (AF, associating foamed alginate matrix and orange peel filler) is successfully tested for fire-retardant properties.
33049885	1	41	dep	foam	71:74	arg1	AF					77:78	AF	77:78	AF	77:78	A new biosourced composite foam (AF, associating foamed alginate matrix and orange peel filler) is successfully tested for fire-retardant properties.
33049885	1	41	dep	foam	71:74	arg1	filler					132:137	orange peel filler	120:137	orange peel filler	120:137	A new biosourced composite foam (AF, associating foamed alginate matrix and orange peel filler) is successfully tested for fire-retardant properties.
33049885	5	42	theme	AF	994:995	arg1	thickness					997:1005	AF thickness	994:1005	AF thickness	994:1005	The investigation of the effect of AF thickness shows that the critical thickness (CT) is close to 1.5-1.7 cm: heat diffusion and material combustion are limited to the CT layer that protects the underlying layers from combustion.
33049885	2	43	theme	thermal	223:229	arg1	properties					242:251	similar thermal insulating properties	215:251	similar thermal insulating properties	215:251	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	0	44	theme	alginate	28:35	arg1	behavior					5:12	Fire behavior	0:12	Fire behavior of innovative alginate	0:35	Fire behavior of innovative alginate foams.
33049885	3	45	theme	promising	627:635	arg1	properties					637:646	the promising properties	623:646	the promising properties of this alternative material	623:675	All these methods confirm the promising properties of this alternative material in terms of fire protection (pHRR, THR, EHC, time-to-ignition, flame duration or production of residue), especially for heat flux not exceeding 50 kW m-2.
33049885	2	46	theme	similar	215:221	arg1	properties					242:251	similar thermal insulating properties	215:251	similar thermal insulating properties	215:251	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	4	47	theme	heat	842:845	arg1	m-2					865:867	75 kW m-2	859:867	75 kW m-2	859:867	At higher heat flux (i.e., 75 kW m-2), flame retardant properties tend to decrease but maintain at a higher level than FR-PUF.
33049885	4	47	theme	heat	842:845	arg1	flux					847:850	higher heat flux	835:850	higher heat flux (i.e., 75 kW m-2)	835:868	At higher heat flux (i.e., 75 kW m-2), flame retardant properties tend to decrease but maintain at a higher level than FR-PUF.
33049885	6	48	theme	negligible	1256:1265	arg1	conduction					1272:1281	negligible heat conduction	1256:1281	: (a) negligible heat conduction	1250:1281	A multiplicity of factors can explain this behavior, such as: (a) negligible heat conduction, (b) low heat of combustion, (c) charring formation, and (d) water release.
33049885	7	49	attach	released	1371:1378	arg2	Water					1359:1363	Water	1359:1363	Water	1359:1363	Water being released from underlying layers, dilutes the gases emitted during the combustion of superficial layers and promotes the flame extinction.
33049885	7	49	attach	released	1371:1378	arg1	layers					1396:1401	underlying layers	1385:1401	underlying layers	1385:1401	Water being released from underlying layers, dilutes the gases emitted during the combustion of superficial layers and promotes the flame extinction.
33049885	1	50	theme	foamed	93:98	arg1	matrix					109:114	associating foamed alginate matrix	81:114	associating foamed alginate matrix	81:114	A new biosourced composite foam (AF, associating foamed alginate matrix and orange peel filler) is successfully tested for fire-retardant properties.
33049885	4	51	theme	higher	933:938	arg1	level					940:944	a higher level	931:944	a higher level than FR-PUF	931:956	At higher heat flux (i.e., 75 kW m-2), flame retardant properties tend to decrease but maintain at a higher level than FR-PUF.
33049885	3	52	theme	flame	740:744	arg1	duration					746:753	flame duration	740:753	flame duration	740:753	All these methods confirm the promising properties of this alternative material in terms of fire protection (pHRR, THR, EHC, time-to-ignition, flame duration or production of residue), especially for heat flux not exceeding 50 kW m-2.
33049885	6	53	theme	factors	1208:1214	arg1	multiplicity					1192:1203	A multiplicity	1190:1203	A multiplicity of factors	1190:1214	A multiplicity of factors can explain this behavior, such as: (a) negligible heat conduction, (b) low heat of combustion, (c) charring formation, and (d) water release.
33049885	2	54	theme	fire-retardant	270:283	arg1	FR-PUF					304:309	FR-PUF	304:309	FR-PUF	304:309	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	2	54	theme	fire-retardant	270:283	arg1	foam					298:301	fire-retardant polyurethane foam	270:301	fire-retardant polyurethane foam (FR-PUF, a commercial product)	270:332	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	1	55	theme	alginate	100:107	arg1	matrix					109:114	associating foamed alginate matrix	81:114	associating foamed alginate matrix	81:114	A new biosourced composite foam (AF, associating foamed alginate matrix and orange peel filler) is successfully tested for fire-retardant properties.
33049885	3	56	theme	50 kW	821:825	arg1	m-2					827:829	50 kW m-2	821:829	50 kW m-2	821:829	All these methods confirm the promising properties of this alternative material in terms of fire protection (pHRR, THR, EHC, time-to-ignition, flame duration or production of residue), especially for heat flux not exceeding 50 kW m-2.
33049885	5	57	theme	CT	1128:1129	arg1	layer					1131:1135	the CT layer	1124:1135	the CT layer that protects the underlying layers from combustion	1124:1187	The investigation of the effect of AF thickness shows that the critical thickness (CT) is close to 1.5-1.7 cm: heat diffusion and material combustion are limited to the CT layer that protects the underlying layers from combustion.
33049885	6	58	theme	low	1288:1290	arg1	heat					1292:1295	(b) low heat of combustion	1284:1309	(b) low heat of combustion	1284:1309	A multiplicity of factors can explain this behavior, such as: (a) negligible heat conduction, (b) low heat of combustion, (c) charring formation, and (d) water release.
33049885	6	59	dep	heat	1292:1295	arg1	b					1285:1285	b	1285:1285	b	1285:1285	A multiplicity of factors can explain this behavior, such as: (a) negligible heat conduction, (b) low heat of combustion, (c) charring formation, and (d) water release.
33049885	3	60	theme	alternative	656:666	arg1	material					668:675	this alternative material	651:675	this alternative material	651:675	All these methods confirm the promising properties of this alternative material in terms of fire protection (pHRR, THR, EHC, time-to-ignition, flame duration or production of residue), especially for heat flux not exceeding 50 kW m-2.
33049885	2	61	theme	commercial	314:323	arg1	product					325:331	a commercial product	312:331	a commercial product	312:331	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	4	62	theme	higher	835:840	arg1	m-2					865:867	75 kW m-2	859:867	75 kW m-2	859:867	At higher heat flux (i.e., 75 kW m-2), flame retardant properties tend to decrease but maintain at a higher level than FR-PUF.
33049885	4	62	theme	higher	835:840	arg1	flux					847:850	higher heat flux	835:850	higher heat flux (i.e., 75 kW m-2)	835:868	At higher heat flux (i.e., 75 kW m-2), flame retardant properties tend to decrease but maintain at a higher level than FR-PUF.
33049885	2	63	contain	having	208:213	arg2	density					257:263	density	257:263	density than fire-retardant polyurethane foam (FR-PUF, a commercial product)	257:332	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	2	63	contain	having	208:213	arg1	material					199:206	This material	194:206	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product)	194:332	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	2	63	contain	having	208:213	arg2	properties					242:251	similar thermal insulating properties	215:251	similar thermal insulating properties	215:251	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	6	64	theme	heat	1267:1270	arg1	conduction					1272:1281	negligible heat conduction	1256:1281	: (a) negligible heat conduction	1250:1281	A multiplicity of factors can explain this behavior, such as: (a) negligible heat conduction, (b) low heat of combustion, (c) charring formation, and (d) water release.
33049885	3	65	theme	material	668:675	arg1	properties					637:646	the promising properties	623:646	the promising properties of this alternative material	623:675	All these methods confirm the promising properties of this alternative material in terms of fire protection (pHRR, THR, EHC, time-to-ignition, flame duration or production of residue), especially for heat flux not exceeding 50 kW m-2.
33049885	1	66	theme	orange	120:125	arg1	filler					132:137	orange peel filler	120:137	orange peel filler	120:137	A new biosourced composite foam (AF, associating foamed alginate matrix and orange peel filler) is successfully tested for fire-retardant properties.
33049885	2	67	theme	flow	488:491	arg1	PCFC					506:509	PCFC	506:509	PCFC	506:509	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	2	67	theme	flow	488:491	arg1	calorimetry					493:503	pyrolysis combustion flow calorimetry	467:503	pyrolysis combustion flow calorimetry (PCFC)	467:510	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	3	68	theme	protection	694:703	arg1	terms					680:684	terms	680:684	terms	680:684	All these methods confirm the promising properties of this alternative material in terms of fire protection (pHRR, THR, EHC, time-to-ignition, flame duration or production of residue), especially for heat flux not exceeding 50 kW m-2.
33049885	2	69	theme	insulating	231:240	arg1	properties					242:251	similar thermal insulating properties	215:251	similar thermal insulating properties	215:251	This material having similar thermal insulating properties and density than fire-retardant polyurethane foam (FR-PUF, a commercial product) shows promising enhanced properties for flame retardancy, as assessed by different methods such as thermogravimetric analysis (TGA), pyrolysis combustion flow calorimetry (PCFC) and a newly designed apparatus called RAPACES for investigating large-scale samples.
33049885	1	70	theme	peel	127:130	arg1	filler					132:137	orange peel filler	120:137	orange peel filler	120:137	A new biosourced composite foam (AF, associating foamed alginate matrix and orange peel filler) is successfully tested for fire-retardant properties.
33049885	3	71	theme	residue	772:778	arg1	time-to-ignition					722:737	time-to-ignition	722:737	time-to-ignition	722:737	All these methods confirm the promising properties of this alternative material in terms of fire protection (pHRR, THR, EHC, time-to-ignition, flame duration or production of residue), especially for heat flux not exceeding 50 kW m-2.
33049885	3	71	theme	residue	772:778	arg1	duration					746:753	flame duration	740:753	flame duration	740:753	All these methods confirm the promising properties of this alternative material in terms of fire protection (pHRR, THR, EHC, time-to-ignition, flame duration or production of residue), especially for heat flux not exceeding 50 kW m-2.
33049885	3	71	theme	residue	772:778	arg1	THR					712:714	THR	712:714	THR	712:714	All these methods confirm the promising properties of this alternative material in terms of fire protection (pHRR, THR, EHC, time-to-ignition, flame duration or production of residue), especially for heat flux not exceeding 50 kW m-2.
33049885	3	71	theme	residue	772:778	arg1	pHRR					706:709	pHRR	706:709	pHRR	706:709	All these methods confirm the promising properties of this alternative material in terms of fire protection (pHRR, THR, EHC, time-to-ignition, flame duration or production of residue), especially for heat flux not exceeding 50 kW m-2.
33049885	3	71	theme	residue	772:778	arg1	production					758:767	production	758:767	production of residue	758:778	All these methods confirm the promising properties of this alternative material in terms of fire protection (pHRR, THR, EHC, time-to-ignition, flame duration or production of residue), especially for heat flux not exceeding 50 kW m-2.
35014492	0	0	theme	Immersed	85:92	arg1	Media					102:106	Immersed Aqueous Media	85:106	Immersed Aqueous Media	85:106	Release of Cellulose Nanocrystal Particles from Natural Rubber Latex Composites into Immersed Aqueous Media.
35014492	3	1	theme	nanocomposite	735:747	arg1	films					749:753	the NR/CNCs nanocomposite films	723:753	the NR/CNCs nanocomposite films	723:753	The release behavior of CNCs from the NR/CNCs nanocomposite films was studied by a fluorescent labeling technique, and the release process in various media was modeled by first-order kinetics.
35014492	4	2	theme	CNCs	901:904	arg1	contents					889:896	Higher contents	882:896	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium	882:974	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	6	3	from	materials	1367:1375	arg1	desirable					1388:1396	desirable	1388:1396	desirable	1388:1396	Nevertheless, in other surroundings, the release of CNCs or any other nanoparticles from composite materials may not be desirable.
35014492	6	3	from	materials	1367:1375	arg1	release					1309:1315	the release	1305:1315	the release of CNCs or any other nanoparticles from composite materials	1305:1375	Nevertheless, in other surroundings, the release of CNCs or any other nanoparticles from composite materials may not be desirable.
35014492	4	4	from	films	1106:1110	arg1	CNCs					1078:1081	CNCs	1078:1081	CNCs from the nanocomposite films	1078:1110	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	4	4	from	films	1106:1110	arg1	release					1067:1073	the release	1063:1073	the release of CNCs from the nanocomposite films	1063:1110	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	4	5	from	CNCs	901:904	arg1	medium					969:974	a relatively acidic or alkaline medium	937:974	a relatively acidic or alkaline medium	937:974	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	4	5	from	CNCs	901:904	arg1	films					927:931	the nanocomposite films	909:931	the nanocomposite films	909:931	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	4	6	from	films	927:931	arg1	contents					889:896	Higher contents	882:896	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium	882:974	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	4	7	from	release	1067:1073	arg1	films					1106:1110	the nanocomposite films	1088:1110	the nanocomposite films	1088:1110	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	3	8	theme	first-order	860:870	arg1	kinetics					872:879	first-order kinetics	860:879	first-order kinetics	860:879	The release behavior of CNCs from the NR/CNCs nanocomposite films was studied by a fluorescent labeling technique, and the release process in various media was modeled by first-order kinetics.
35014492	1	9	from	materials	289:297	arg1	release					262:268	the release	258:268	the release of CNCs from those materials into aqueous solutions	258:320	In the current study, we focused on the preparation of nanocomposite films of natural rubber latex-cellulose nanocrystals (NR/CNCs) and investigated the release of CNCs from those materials into aqueous solutions.
35014492	7	10	from	matrix	1498:1503	arg1	release					1461:1467	the release	1457:1467	the release of nanoparticles from a host matrix into the surrounding media	1457:1530	Hence, this study also provides a protocol to investigate the release of nanoparticles from a host matrix into the surrounding media and also promotes a rethinking of the nanoparticle release issue from composites to the environment.
35014492	4	11	theme	CNCs	1078:1081	arg1	release					1067:1073	the release	1063:1073	the release of CNCs from the nanocomposite films	1063:1110	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	0	12	theme	Aqueous	94:100	arg1	Media					102:106	Immersed Aqueous Media	85:106	Immersed Aqueous Media	85:106	Release of Cellulose Nanocrystal Particles from Natural Rubber Latex Composites into Immersed Aqueous Media.
35014492	4	13	from	contents	889:896	arg1	medium					969:974	a relatively acidic or alkaline medium	937:974	a relatively acidic or alkaline medium	937:974	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	4	13	from	contents	889:896	arg1	films					927:931	the nanocomposite films	909:931	the nanocomposite films	909:931	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	6	14	theme	other	1285:1289	arg1	surroundings					1291:1302	other surroundings	1285:1302	other surroundings	1285:1302	Nevertheless, in other surroundings, the release of CNCs or any other nanoparticles from composite materials may not be desirable.
35014492	4	15	theme	ionic	1022:1026	arg1	strength					1028:1035	higher ionic strength	1015:1035	higher ionic strength of the media	1015:1048	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	4	16	from	medium	969:974	arg1	contents					889:896	Higher contents	882:896	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium	882:974	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	4	17	theme	release	992:998	arg1	process					1000:1006	the release process	988:1006	the release process	988:1006	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	2	18	theme	dynamic	661:667	arg1	testing					680:686	tensile and dynamic mechanical testing	649:686	tensile and dynamic mechanical testing	649:686	The obtained nanocomposite films were well characterized for further understanding of the release mechanism; as the intermolecular interactions between the two components were studied by Fourier-transform infrared spectroscopy, the morphology was studied with scanning electron microscopy, and nanostructures were analyzed by tensile and dynamic mechanical testing.
35014492	2	19	theme	mechanism	421:429	arg1	understanding					392:404	further understanding	384:404	further understanding of the release mechanism	384:429	The obtained nanocomposite films were well characterized for further understanding of the release mechanism; as the intermolecular interactions between the two components were studied by Fourier-transform infrared spectroscopy, the morphology was studied with scanning electron microscopy, and nanostructures were analyzed by tensile and dynamic mechanical testing.
35014492	2	20	theme	further	384:390	arg1	understanding					392:404	further understanding	384:404	further understanding of the release mechanism	384:429	The obtained nanocomposite films were well characterized for further understanding of the release mechanism; as the intermolecular interactions between the two components were studied by Fourier-transform infrared spectroscopy, the morphology was studied with scanning electron microscopy, and nanostructures were analyzed by tensile and dynamic mechanical testing.
35014492	2	21	theme	infrared	528:535	arg1	spectroscopy					537:548	Fourier-transform infrared spectroscopy	510:548	Fourier-transform infrared spectroscopy	510:548	The obtained nanocomposite films were well characterized for further understanding of the release mechanism; as the intermolecular interactions between the two components were studied by Fourier-transform infrared spectroscopy, the morphology was studied with scanning electron microscopy, and nanostructures were analyzed by tensile and dynamic mechanical testing.
35014492	5	22	from	composites	1185:1194	arg1	particles					1163:1171	CNC particles	1159:1171	CNC particles from NR/CNC composites	1159:1194	In this study, our objective was to transport CNC particles from NR/CNC composites into immersed media to be used beneficially in biomedical applications.
35014492	6	23	theme	composite	1357:1365	arg1	materials					1367:1375	composite materials	1357:1375	composite materials	1357:1375	Nevertheless, in other surroundings, the release of CNCs or any other nanoparticles from composite materials may not be desirable.
35014492	4	24	theme	media	1044:1048	arg1	strength					1028:1035	higher ionic strength	1015:1035	higher ionic strength of the media	1015:1048	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	6	25	from	surroundings	1291:1302	arg1	desirable					1388:1396	desirable	1388:1396	desirable	1388:1396	Nevertheless, in other surroundings, the release of CNCs or any other nanoparticles from composite materials may not be desirable.
35014492	6	25	from	surroundings	1291:1302	arg1	release					1309:1315	the release	1305:1315	the release of CNCs or any other nanoparticles from composite materials	1305:1375	Nevertheless, in other surroundings, the release of CNCs or any other nanoparticles from composite materials may not be desirable.
35014492	3	26	from	films	749:753	arg1	behavior					701:708	The release behavior	689:708	The release behavior of CNCs from the NR/CNCs nanocomposite films	689:753	The release behavior of CNCs from the NR/CNCs nanocomposite films was studied by a fluorescent labeling technique, and the release process in various media was modeled by first-order kinetics.
35014492	3	26	from	films	749:753	arg1	CNCs					713:716	CNCs	713:716	CNCs from the NR/CNCs nanocomposite films	713:753	The release behavior of CNCs from the NR/CNCs nanocomposite films was studied by a fluorescent labeling technique, and the release process in various media was modeled by first-order kinetics.
35014492	3	27	theme	fluorescent	772:782	arg1	technique					793:801	a fluorescent labeling technique	770:801	a fluorescent labeling technique	770:801	The release behavior of CNCs from the NR/CNCs nanocomposite films was studied by a fluorescent labeling technique, and the release process in various media was modeled by first-order kinetics.
35014492	2	28	theme	tensile	649:655	arg1	testing					680:686	tensile and dynamic mechanical testing	649:686	tensile and dynamic mechanical testing	649:686	The obtained nanocomposite films were well characterized for further understanding of the release mechanism; as the intermolecular interactions between the two components were studied by Fourier-transform infrared spectroscopy, the morphology was studied with scanning electron microscopy, and nanostructures were analyzed by tensile and dynamic mechanical testing.
35014492	5	29	theme	NR/CNC	1178:1183	arg1	composites					1185:1194	NR/CNC composites	1178:1194	NR/CNC composites	1178:1194	In this study, our objective was to transport CNC particles from NR/CNC composites into immersed media to be used beneficially in biomedical applications.
35014492	6	30	theme	CNCs	1320:1323	arg1	desirable					1388:1396	desirable	1388:1396	desirable	1388:1396	Nevertheless, in other surroundings, the release of CNCs or any other nanoparticles from composite materials may not be desirable.
35014492	6	30	theme	CNCs	1320:1323	arg1	release					1309:1315	the release	1305:1315	the release of CNCs or any other nanoparticles from composite materials	1305:1375	Nevertheless, in other surroundings, the release of CNCs or any other nanoparticles from composite materials may not be desirable.
35014492	1	31	theme	nanocomposite	164:176	arg1	films					178:182	nanocomposite films	164:182	nanocomposite films of natural rubber latex-cellulose nanocrystals (NR/CNCs)	164:239	In the current study, we focused on the preparation of nanocomposite films of natural rubber latex-cellulose nanocrystals (NR/CNCs) and investigated the release of CNCs from those materials into aqueous solutions.
35014492	2	32	theme	intermolecular	439:452	arg1	interactions					454:465	the intermolecular interactions	435:465	the intermolecular interactions between the two components	435:492	The obtained nanocomposite films were well characterized for further understanding of the release mechanism; as the intermolecular interactions between the two components were studied by Fourier-transform infrared spectroscopy, the morphology was studied with scanning electron microscopy, and nanostructures were analyzed by tensile and dynamic mechanical testing.
35014492	3	33	theme	labeling	784:791	arg1	technique					793:801	a fluorescent labeling technique	770:801	a fluorescent labeling technique	770:801	The release behavior of CNCs from the NR/CNCs nanocomposite films was studied by a fluorescent labeling technique, and the release process in various media was modeled by first-order kinetics.
35014492	4	34	theme	Higher	882:887	arg1	contents					889:896	Higher contents	882:896	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium	882:974	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	7	35	theme	host	1493:1496	arg1	matrix					1498:1503	a host matrix	1491:1503	a host matrix	1491:1503	Hence, this study also provides a protocol to investigate the release of nanoparticles from a host matrix into the surrounding media and also promotes a rethinking of the nanoparticle release issue from composites to the environment.
35014492	1	36	theme	films	178:182	arg1	preparation					149:159	the preparation	145:159	the preparation of nanocomposite films of natural rubber latex-cellulose nanocrystals (NR/CNCs)	145:239	In the current study, we focused on the preparation of nanocomposite films of natural rubber latex-cellulose nanocrystals (NR/CNCs) and investigated the release of CNCs from those materials into aqueous solutions.
35014492	0	37	theme	Nanocrystal	21:31	arg1	Particles					33:41	Cellulose Nanocrystal Particles	11:41	Cellulose Nanocrystal Particles	11:41	Release of Cellulose Nanocrystal Particles from Natural Rubber Latex Composites into Immersed Aqueous Media.
35014492	1	38	theme	CNCs	273:276	arg1	release					262:268	the release	258:268	the release of CNCs from those materials into aqueous solutions	258:320	In the current study, we focused on the preparation of nanocomposite films of natural rubber latex-cellulose nanocrystals (NR/CNCs) and investigated the release of CNCs from those materials into aqueous solutions.
35014492	4	39	theme	acidic	950:955	arg1	medium					969:974	a relatively acidic or alkaline medium	937:974	a relatively acidic or alkaline medium	937:974	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	2	40	theme	mechanical	669:678	arg1	testing					680:686	tensile and dynamic mechanical testing	649:686	tensile and dynamic mechanical testing	649:686	The obtained nanocomposite films were well characterized for further understanding of the release mechanism; as the intermolecular interactions between the two components were studied by Fourier-transform infrared spectroscopy, the morphology was studied with scanning electron microscopy, and nanostructures were analyzed by tensile and dynamic mechanical testing.
35014492	4	41	theme	higher	1015:1020	arg1	strength					1028:1035	higher ionic strength	1015:1035	higher ionic strength of the media	1015:1048	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	0	42	theme	Cellulose	11:19	arg1	Particles					33:41	Cellulose Nanocrystal Particles	11:41	Cellulose Nanocrystal Particles	11:41	Release of Cellulose Nanocrystal Particles from Natural Rubber Latex Composites into Immersed Aqueous Media.
35014492	7	43	dep	release	1583:1589	arg1	issue					1591:1595	issue	1591:1595	the nanoparticle release issue from composites to the environment	1566:1630	Hence, this study also provides a protocol to investigate the release of nanoparticles from a host matrix into the surrounding media and also promotes a rethinking of the nanoparticle release issue from composites to the environment.
35014492	6	44	theme	nanoparticles	1338:1350	arg1	desirable					1388:1396	desirable	1388:1396	desirable	1388:1396	Nevertheless, in other surroundings, the release of CNCs or any other nanoparticles from composite materials may not be desirable.
35014492	6	44	theme	nanoparticles	1338:1350	arg1	release					1309:1315	the release	1305:1315	the release of CNCs or any other nanoparticles from composite materials	1305:1375	Nevertheless, in other surroundings, the release of CNCs or any other nanoparticles from composite materials may not be desirable.
35014492	1	45	theme	natural	187:193	arg1	NR/CNCs					232:238	NR/CNCs	232:238	NR/CNCs	232:238	In the current study, we focused on the preparation of nanocomposite films of natural rubber latex-cellulose nanocrystals (NR/CNCs) and investigated the release of CNCs from those materials into aqueous solutions.
35014492	1	45	theme	natural	187:193	arg1	nanocrystals					218:229	natural rubber latex-cellulose nanocrystals	187:229	natural rubber latex-cellulose nanocrystals (NR/CNCs)	187:239	In the current study, we focused on the preparation of nanocomposite films of natural rubber latex-cellulose nanocrystals (NR/CNCs) and investigated the release of CNCs from those materials into aqueous solutions.
35014492	5	46	theme	immersed	1201:1208	arg1	media					1210:1214	immersed media	1201:1214	immersed media	1201:1214	In this study, our objective was to transport CNC particles from NR/CNC composites into immersed media to be used beneficially in biomedical applications.
35014492	2	47	theme	obtained	327:334	arg1	films					350:354	The obtained nanocomposite films	323:354	The obtained nanocomposite films	323:354	The obtained nanocomposite films were well characterized for further understanding of the release mechanism; as the intermolecular interactions between the two components were studied by Fourier-transform infrared spectroscopy, the morphology was studied with scanning electron microscopy, and nanostructures were analyzed by tensile and dynamic mechanical testing.
35014492	4	48	theme	alkaline	960:967	arg1	medium					969:974	a relatively acidic or alkaline medium	937:974	a relatively acidic or alkaline medium	937:974	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	1	49	theme	rubber	195:200	arg1	NR/CNCs					232:238	NR/CNCs	232:238	NR/CNCs	232:238	In the current study, we focused on the preparation of nanocomposite films of natural rubber latex-cellulose nanocrystals (NR/CNCs) and investigated the release of CNCs from those materials into aqueous solutions.
35014492	1	49	theme	rubber	195:200	arg1	nanocrystals					218:229	natural rubber latex-cellulose nanocrystals	187:229	natural rubber latex-cellulose nanocrystals (NR/CNCs)	187:239	In the current study, we focused on the preparation of nanocomposite films of natural rubber latex-cellulose nanocrystals (NR/CNCs) and investigated the release of CNCs from those materials into aqueous solutions.
35014492	2	50	theme	release	413:419	arg1	mechanism					421:429	the release mechanism	409:429	the release mechanism	409:429	The obtained nanocomposite films were well characterized for further understanding of the release mechanism; as the intermolecular interactions between the two components were studied by Fourier-transform infrared spectroscopy, the morphology was studied with scanning electron microscopy, and nanostructures were analyzed by tensile and dynamic mechanical testing.
35014492	0	51	theme	Particles	33:41	arg1	Release					0:6	Release	0:6	Release of Cellulose Nanocrystal Particles from Natural Rubber Latex	0:67	Release of Cellulose Nanocrystal Particles from Natural Rubber Latex Composites into Immersed Aqueous Media.
35014492	7	52	theme	nanoparticles	1472:1484	arg1	release					1461:1467	the release	1457:1467	the release of nanoparticles from a host matrix into the surrounding media	1457:1530	Hence, this study also provides a protocol to investigate the release of nanoparticles from a host matrix into the surrounding media and also promotes a rethinking of the nanoparticle release issue from composites to the environment.
35014492	4	53	theme	or	957:958	arg1	medium					969:974	a relatively acidic or alkaline medium	937:974	a relatively acidic or alkaline medium	937:974	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	6	54	theme	other	1332:1336	arg1	nanoparticles					1338:1350	any other nanoparticles	1328:1350	any other nanoparticles	1328:1350	Nevertheless, in other surroundings, the release of CNCs or any other nanoparticles from composite materials may not be desirable.
35014492	7	55	theme	nanoparticle	1570:1581	arg1	release					1583:1589	the nanoparticle release	1566:1589	the nanoparticle release issue from composites to the environment	1566:1630	Hence, this study also provides a protocol to investigate the release of nanoparticles from a host matrix into the surrounding media and also promotes a rethinking of the nanoparticle release issue from composites to the environment.
35014492	1	56	theme	latex-cellulose	202:216	arg1	NR/CNCs					232:238	NR/CNCs	232:238	NR/CNCs	232:238	In the current study, we focused on the preparation of nanocomposite films of natural rubber latex-cellulose nanocrystals (NR/CNCs) and investigated the release of CNCs from those materials into aqueous solutions.
35014492	1	56	theme	latex-cellulose	202:216	arg1	nanocrystals					218:229	natural rubber latex-cellulose nanocrystals	187:229	natural rubber latex-cellulose nanocrystals (NR/CNCs)	187:239	In the current study, we focused on the preparation of nanocomposite films of natural rubber latex-cellulose nanocrystals (NR/CNCs) and investigated the release of CNCs from those materials into aqueous solutions.
35014492	0	57	theme	Rubber	56:61	arg1	Latex					63:67	Natural Rubber Latex	48:67	Natural Rubber Latex	48:67	Release of Cellulose Nanocrystal Particles from Natural Rubber Latex Composites into Immersed Aqueous Media.
35014492	3	58	theme	release	812:818	arg1	process					820:826	the release process	808:826	the release process in various media	808:843	The release behavior of CNCs from the NR/CNCs nanocomposite films was studied by a fluorescent labeling technique, and the release process in various media was modeled by first-order kinetics.
35014492	3	59	from	process	820:826	arg1	media					839:843	various media	831:843	various media	831:843	The release behavior of CNCs from the NR/CNCs nanocomposite films was studied by a fluorescent labeling technique, and the release process in various media was modeled by first-order kinetics.
35014492	0	60	from	Latex	63:67	arg1	Release					0:6	Release	0:6	Release of Cellulose Nanocrystal Particles from Natural Rubber Latex	0:67	Release of Cellulose Nanocrystal Particles from Natural Rubber Latex Composites into Immersed Aqueous Media.
35014492	2	61	theme	nanocomposite	336:348	arg1	films					350:354	The obtained nanocomposite films	323:354	The obtained nanocomposite films	323:354	The obtained nanocomposite films were well characterized for further understanding of the release mechanism; as the intermolecular interactions between the two components were studied by Fourier-transform infrared spectroscopy, the morphology was studied with scanning electron microscopy, and nanostructures were analyzed by tensile and dynamic mechanical testing.
35014492	0	62	theme	Natural	48:54	arg1	Latex					63:67	Natural Rubber Latex	48:67	Natural Rubber Latex	48:67	Release of Cellulose Nanocrystal Particles from Natural Rubber Latex Composites into Immersed Aqueous Media.
35014492	1	63	theme	aqueous	304:310	arg1	solutions					312:320	aqueous solutions	304:320	aqueous solutions	304:320	In the current study, we focused on the preparation of nanocomposite films of natural rubber latex-cellulose nanocrystals (NR/CNCs) and investigated the release of CNCs from those materials into aqueous solutions.
35014492	4	64	theme	nanocomposite	913:925	arg1	films					927:931	the nanocomposite films	909:931	the nanocomposite films	909:931	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	7	65	theme	surrounding	1514:1524	arg1	media					1526:1530	the surrounding media	1510:1530	the surrounding media	1510:1530	Hence, this study also provides a protocol to investigate the release of nanoparticles from a host matrix into the surrounding media and also promotes a rethinking of the nanoparticle release issue from composites to the environment.
35014492	1	66	theme	current	116:122	arg1	study					124:128	the current study	112:128	the current study	112:128	In the current study, we focused on the preparation of nanocomposite films of natural rubber latex-cellulose nanocrystals (NR/CNCs) and investigated the release of CNCs from those materials into aqueous solutions.
35014492	4	67	theme	nanocomposite	1092:1104	arg1	films					1106:1110	the nanocomposite films	1088:1110	the nanocomposite films	1088:1110	Higher contents of CNCs in the nanocomposite films and a relatively acidic or alkaline medium facilitated the release process, while higher ionic strength of the media could hamper the release of CNCs from the nanocomposite films.
35014492	7	68	from	rethinking	1552:1561	arg1	composites					1602:1611	composites	1602:1611	composites	1602:1611	Hence, this study also provides a protocol to investigate the release of nanoparticles from a host matrix into the surrounding media and also promotes a rethinking of the nanoparticle release issue from composites to the environment.
35014492	2	69	theme	electron	592:599	arg1	microscopy					601:610	scanning electron microscopy	583:610	scanning electron microscopy	583:610	The obtained nanocomposite films were well characterized for further understanding of the release mechanism; as the intermolecular interactions between the two components were studied by Fourier-transform infrared spectroscopy, the morphology was studied with scanning electron microscopy, and nanostructures were analyzed by tensile and dynamic mechanical testing.
35014492	3	70	theme	CNCs	713:716	arg1	behavior					701:708	The release behavior	689:708	The release behavior of CNCs from the NR/CNCs nanocomposite films	689:753	The release behavior of CNCs from the NR/CNCs nanocomposite films was studied by a fluorescent labeling technique, and the release process in various media was modeled by first-order kinetics.
35014492	1	71	theme	nanocrystals	218:229	arg1	films					178:182	nanocomposite films	164:182	nanocomposite films of natural rubber latex-cellulose nanocrystals (NR/CNCs)	164:239	In the current study, we focused on the preparation of nanocomposite films of natural rubber latex-cellulose nanocrystals (NR/CNCs) and investigated the release of CNCs from those materials into aqueous solutions.
35014492	2	72	theme	scanning	583:590	arg1	microscopy					601:610	scanning electron microscopy	583:610	scanning electron microscopy	583:610	The obtained nanocomposite films were well characterized for further understanding of the release mechanism; as the intermolecular interactions between the two components were studied by Fourier-transform infrared spectroscopy, the morphology was studied with scanning electron microscopy, and nanostructures were analyzed by tensile and dynamic mechanical testing.
35014492	3	73	theme	release	693:699	arg1	behavior					701:708	The release behavior	689:708	The release behavior of CNCs from the NR/CNCs nanocomposite films	689:753	The release behavior of CNCs from the NR/CNCs nanocomposite films was studied by a fluorescent labeling technique, and the release process in various media was modeled by first-order kinetics.
35014492	7	74	from	composites	1602:1611	arg1	release					1583:1589	the nanoparticle release	1566:1589	the nanoparticle release issue from composites to the environment	1566:1630	Hence, this study also provides a protocol to investigate the release of nanoparticles from a host matrix into the surrounding media and also promotes a rethinking of the nanoparticle release issue from composites to the environment.
35014492	7	74	from	composites	1602:1611	arg1	rethinking					1552:1561	a rethinking	1550:1561	a rethinking of the nanoparticle release issue from composites to the environment	1550:1630	Hence, this study also provides a protocol to investigate the release of nanoparticles from a host matrix into the surrounding media and also promotes a rethinking of the nanoparticle release issue from composites to the environment.
35014492	2	75	theme	Fourier-transform	510:526	arg1	spectroscopy					537:548	Fourier-transform infrared spectroscopy	510:548	Fourier-transform infrared spectroscopy	510:548	The obtained nanocomposite films were well characterized for further understanding of the release mechanism; as the intermolecular interactions between the two components were studied by Fourier-transform infrared spectroscopy, the morphology was studied with scanning electron microscopy, and nanostructures were analyzed by tensile and dynamic mechanical testing.
35014492	6	76	from	desirable	1388:1396	arg1	surroundings					1291:1302	other surroundings	1285:1302	other surroundings	1285:1302	Nevertheless, in other surroundings, the release of CNCs or any other nanoparticles from composite materials may not be desirable.
35014492	5	77	theme	biomedical	1243:1252	arg1	applications					1254:1265	biomedical applications	1243:1265	biomedical applications	1243:1265	In this study, our objective was to transport CNC particles from NR/CNC composites into immersed media to be used beneficially in biomedical applications.
35014492	3	78	from	behavior	701:708	arg1	films					749:753	the NR/CNCs nanocomposite films	723:753	the NR/CNCs nanocomposite films	723:753	The release behavior of CNCs from the NR/CNCs nanocomposite films was studied by a fluorescent labeling technique, and the release process in various media was modeled by first-order kinetics.
35014492	3	79	theme	NR/CNCs	727:733	arg1	films					749:753	the NR/CNCs nanocomposite films	723:753	the NR/CNCs nanocomposite films	723:753	The release behavior of CNCs from the NR/CNCs nanocomposite films was studied by a fluorescent labeling technique, and the release process in various media was modeled by first-order kinetics.
35014492	7	80	theme	release	1583:1589	arg1	rethinking					1552:1561	a rethinking	1550:1561	a rethinking of the nanoparticle release issue from composites to the environment	1550:1630	Hence, this study also provides a protocol to investigate the release of nanoparticles from a host matrix into the surrounding media and also promotes a rethinking of the nanoparticle release issue from composites to the environment.
35014492	3	81	theme	various	831:837	arg1	media					839:843	various media	831:843	various media	831:843	The release behavior of CNCs from the NR/CNCs nanocomposite films was studied by a fluorescent labeling technique, and the release process in various media was modeled by first-order kinetics.
35014492	5	82	theme	CNC	1159:1161	arg1	particles					1163:1171	CNC particles	1159:1171	CNC particles from NR/CNC composites	1159:1194	In this study, our objective was to transport CNC particles from NR/CNC composites into immersed media to be used beneficially in biomedical applications.
34074422	8	0	theme	side-to-side	1743:1754	arg1	comparison					1756:1765	side-to-side comparison	1743:1765	side-to-side comparison of differential expression results with other method	1743:1818	For these 153 intact N-glycoproteins, the molecular functions and biological processes of were comprehensively discussed, and side-to-side comparison of differential expression results with other method were also made.
34074422	4	1	theme	expressed	899:907	arg1	DEGPs					933:937	DEGPs	933:937	DEGPs	933:937	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	4	1	theme	expressed	899:907	arg1	N-glycopeptides					916:930	differentially expressed intact N-glycopeptides	884:930	differentially expressed intact N-glycopeptides (DEGPs)	884:938	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	8	2	theme	biological	1683:1692	arg1	processes					1694:1702	biological processes	1683:1702	biological processes	1683:1702	For these 153 intact N-glycoproteins, the molecular functions and biological processes of were comprehensively discussed, and side-to-side comparison of differential expression results with other method were also made.
34074422	3	3	contain	have	393:396	arg1	cells					380:384	Cancer stem cells	368:384	Cancer stem cells (CSCs)	368:391	Cancer stem cells (CSCs) have the remarkable potential of self-renewing and differentiation which leads to drug resistance and metastasis.
34074422	3	3	contain	have	393:396	arg1	CSCs					387:390	CSCs	387:390	CSCs	387:390	Cancer stem cells (CSCs) have the remarkable potential of self-renewing and differentiation which leads to drug resistance and metastasis.
34074422	3	3	contain	have	393:396	arg2	potential					413:421	the remarkable potential	398:421	the remarkable potential of self-renewing and differentiation which leads to drug resistance and metastasis	398:504	Cancer stem cells (CSCs) have the remarkable potential of self-renewing and differentiation which leads to drug resistance and metastasis.
34074422	7	4	theme	fold	1465:1468	arg1	change≥1.5					1470:1479	fold change≥1.5	1465:1479	fold change≥1.5	1465:1479	With the three technical replicates and the criteria of fold change≥1.5 and p value<0.05, 380 DEGPs (corresponding to 153 intact N-glycoproteins) were found along with 293 down-regulated and 87 up-regulated.
34074422	4	5	theme	putative	669:676	arg1	biomarkers					678:687	the putative biomarkers	665:687	the putative biomarkers	665:687	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	8	6	theme	expression	1783:1792	arg1	results					1794:1800	differential expression results	1770:1800	differential expression results	1770:1800	For these 153 intact N-glycoproteins, the molecular functions and biological processes of were comprehensively discussed, and side-to-side comparison of differential expression results with other method were also made.
34074422	1	7	theme	diseases	172:179	arg1	diseases					172:179	the most malignant diseases	153:179	the most malignant diseases among females	153:193	Breast cancer is one of the most malignant diseases among females.
34074422	1	7	theme	diseases	172:179	arg1	one					146:148	one	146:148	one	146:148	Breast cancer is one of the most malignant diseases among females.
34074422	4	8	dep	CSCs	626:629	arg1	relative					632:639	relative	632:639	relative	632:639	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	2	9	theme	cancer	301:306	arg1	progression					308:318	cancer progression	301:318	cancer progression	301:318	N-glycoproteomics studies have shown that N-glycosylation alteration of tumor cells is the key player of cancer progression, multidrug resistance (MDR) and high mortality.
34074422	0	10	from	characterization	86:101	arg1	markers					24:30	Putative N-glycoprotein markers	0:30	Putative N-glycoprotein markers of MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate.	0:127	Putative N-glycoprotein markers of MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate.
34074422	0	10	from	characterization	86:101	arg1	cells					57:61	MCF-7/ADR cancer stem cells	35:61	MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate	35:126	Putative N-glycoprotein markers of MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate.
34074422	5	11	dep	1737 N-glycosites	1083:1099	arg1	linkages					1180:1187	181 putative N-glycan linkages	1158:1187	181 putative N-glycan linkages	1158:1187	With control of spectrum-level FDR≤1%, 5515 intact N-glycopeptides were identified (1737 N-glycosites, 1705 peptide backbones and 1516 intact N-glycoproteins; 181 putative N-glycan linkages and 68 monosaccharide compositions).
34074422	5	11	dep	1737 N-glycosites	1083:1099	arg1	compositions					1211:1222	68 monosaccharide compositions	1193:1222	68 monosaccharide compositions	1193:1222	With control of spectrum-level FDR≤1%, 5515 intact N-glycopeptides were identified (1737 N-glycosites, 1705 peptide backbones and 1516 intact N-glycoproteins; 181 putative N-glycan linkages and 68 monosaccharide compositions).
34074422	5	12	theme	peptide	1107:1113	arg1	backbones					1115:1123	1705 peptide backbones	1102:1123	1705 peptide backbones	1102:1123	With control of spectrum-level FDR≤1%, 5515 intact N-glycopeptides were identified (1737 N-glycosites, 1705 peptide backbones and 1516 intact N-glycoproteins; 181 putative N-glycan linkages and 68 monosaccharide compositions).
34074422	2	13	theme	N-glycosylation	238:252	arg1	player					291:296	the key player	283:296	the key player of cancer progression	283:318	N-glycoproteomics studies have shown that N-glycosylation alteration of tumor cells is the key player of cancer progression, multidrug resistance (MDR) and high mortality.
34074422	2	13	theme	N-glycosylation	238:252	arg1	alteration					254:263	N-glycosylation alteration	238:263	N-glycosylation alteration of tumor cells	238:278	N-glycoproteomics studies have shown that N-glycosylation alteration of tumor cells is the key player of cancer progression, multidrug resistance (MDR) and high mortality.
34074422	4	14	theme	isotopic	731:738	arg1	N-glycopeptides					771:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides	690:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs	690:820	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	6	15	theme	fragment	1348:1355	arg1	ions					1357:1360	one or more structure-diagnostic fragment ions	1315:1360	one or more structure-diagnostic fragment ions	1315:1360	Among 5515 intact N-glycopeptide IDs, 3864 were identified with glycoform score≥1, i.e., one or more structure-diagnostic fragment ions were observed to distinguish sequence isomers.
34074422	1	16	theme	Breast	129:134	arg1	cancer					136:141	Breast cancer	129:141	Breast cancer	129:141	Breast cancer is one of the most malignant diseases among females.
34074422	4	17	theme	labelled	748:755	arg1	N-glycopeptides					771:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides	690:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs	690:820	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	2	18	theme	cells	274:278	arg1	player					291:296	the key player	283:296	the key player of cancer progression	283:318	N-glycoproteomics studies have shown that N-glycosylation alteration of tumor cells is the key player of cancer progression, multidrug resistance (MDR) and high mortality.
34074422	2	18	theme	cells	274:278	arg1	alteration					254:263	N-glycosylation alteration	238:263	N-glycosylation alteration of tumor cells	238:278	N-glycoproteomics studies have shown that N-glycosylation alteration of tumor cells is the key player of cancer progression, multidrug resistance (MDR) and high mortality.
34074422	7	19	gly	N-glycoproteins	1538:1552	arg1	N-glycoproteins					1538:1552	153 intact N-glycoproteins	1527:1552	153 intact N-glycoproteins	1527:1552	With the three technical replicates and the criteria of fold change≥1.5 and p value<0.05, 380 DEGPs (corresponding to 153 intact N-glycoproteins) were found along with 293 down-regulated and 87 up-regulated.
34074422	6	20	theme	intact	1237:1242	arg1	IDs					1259:1261	5515 intact N-glycopeptide IDs	1232:1261	5515 intact N-glycopeptide IDs	1232:1261	Among 5515 intact N-glycopeptide IDs, 3864 were identified with glycoform score≥1, i.e., one or more structure-diagnostic fragment ions were observed to distinguish sequence isomers.
34074422	4	21	theme	paired	694:699	arg1	N-glycopeptides					771:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides	690:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs	690:820	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	4	22	gly	N-glycopeptides	916:930	arg2	DEGPs					933:937	DEGPs	933:937	DEGPs	933:937	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	4	22	gly	N-glycopeptides	916:930	arg2	N-glycopeptides					916:930	differentially expressed intact N-glycopeptides	884:930	differentially expressed intact N-glycopeptides (DEGPs)	884:938	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	0	23	from	markers	24:30	arg1	characterization					86:101	N-glycoproteomics characterization	68:101	N-glycoproteomics characterization of the whole cell lysate	68:126	Putative N-glycoprotein markers of MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate.
34074422	5	24	theme	putative	1162:1169	arg1	linkages					1180:1187	181 putative N-glycan linkages	1158:1187	181 putative N-glycan linkages	1158:1187	With control of spectrum-level FDR≤1%, 5515 intact N-glycopeptides were identified (1737 N-glycosites, 1705 peptide backbones and 1516 intact N-glycoproteins; 181 putative N-glycan linkages and 68 monosaccharide compositions).
34074422	4	25	from	CSCs	802:805	arg1	N-glycopeptides					771:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides	690:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs	690:820	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	4	26	theme	engine	982:987	arg1	GPSeeker					989:996	search engine GPSeeker	975:996	search engine GPSeeker	975:996	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	0	27	theme	cell	116:119	arg1	lysate					121:126	the whole cell lysate	106:126	the whole cell lysate	106:126	Putative N-glycoprotein markers of MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate.
34074422	5	28	theme	intact	1134:1139	arg1	N-glycoproteins					1141:1155	1516 intact N-glycoproteins	1129:1155	1516 intact N-glycoproteins	1129:1155	With control of spectrum-level FDR≤1%, 5515 intact N-glycopeptides were identified (1737 N-glycosites, 1705 peptide backbones and 1516 intact N-glycoproteins; 181 putative N-glycan linkages and 68 monosaccharide compositions).
34074422	0	29	theme	cancer	45:50	arg1	cells					57:61	MCF-7/ADR cancer stem cells	35:61	MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate	35:126	Putative N-glycoprotein markers of MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate.
34074422	0	30	theme	N-glycoproteomics	68:84	arg1	characterization					86:101	N-glycoproteomics characterization	68:101	N-glycoproteomics characterization of the whole cell lysate	68:126	Putative N-glycoprotein markers of MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate.
34074422	4	31	theme	SIDE	758:761	arg1	N-glycopeptides					771:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides	690:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs	690:820	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	3	32	theme	remarkable	402:411	arg1	potential					413:421	the remarkable potential	398:421	the remarkable potential of self-renewing and differentiation which leads to drug resistance and metastasis	398:504	Cancer stem cells (CSCs) have the remarkable potential of self-renewing and differentiation which leads to drug resistance and metastasis.
34074422	0	33	theme	Putative	0:7	arg1	markers					24:30	Putative N-glycoprotein markers	0:30	Putative N-glycoprotein markers of MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate.	0:127	Putative N-glycoprotein markers of MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate.
34074422	3	34	theme	Cancer	368:373	arg1	cells					380:384	Cancer stem cells	368:384	Cancer stem cells (CSCs)	368:391	Cancer stem cells (CSCs) have the remarkable potential of self-renewing and differentiation which leads to drug resistance and metastasis.
34074422	3	34	theme	Cancer	368:373	arg1	CSCs					387:390	CSCs	387:390	CSCs	387:390	Cancer stem cells (CSCs) have the remarkable potential of self-renewing and differentiation which leads to drug resistance and metastasis.
34074422	4	35	theme	intact	764:769	arg1	N-glycopeptides					771:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides	690:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs	690:820	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	4	36	theme	breast	591:596	arg1	cells					610:614	adriamycin-resistant breast cancer stem cells	570:614	adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs)	570:654	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	0	37	theme	MCF-7/ADR	35:43	arg1	cells					57:61	MCF-7/ADR cancer stem cells	35:61	MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate	35:126	Putative N-glycoprotein markers of MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate.
34074422	0	38	gly	N-glycoprotein	9:22	arg1	N-glycoprotein					9:22	N-glycoprotein	9:22	N-glycoprotein	9:22	Putative N-glycoprotein markers of MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate.
34074422	0	39	theme	stem	52:55	arg1	cells					57:61	MCF-7/ADR cancer stem cells	35:61	MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate	35:126	Putative N-glycoprotein markers of MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate.
34074422	4	40	theme	expressed	541:549	arg1	N-glycosylation					551:565	the differentially expressed N-glycosylation	522:565	the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs)	522:654	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	4	41	theme	MCF-7	811:815	arg1	CSCs					817:820	MCF-7 CSCs	811:820	MCF-7 CSCs	811:820	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	8	42	gly	N-glycoproteins	1638:1652	arg1	N-glycoproteins					1638:1652	these 153 intact N-glycoproteins	1621:1652	these 153 intact N-glycoproteins	1621:1652	For these 153 intact N-glycoproteins, the molecular functions and biological processes of were comprehensively discussed, and side-to-side comparison of differential expression results with other method were also made.
34074422	7	43	theme	intact	1531:1536	arg1	N-glycoproteins					1538:1552	153 intact N-glycoproteins	1527:1552	153 intact N-glycoproteins	1527:1552	With the three technical replicates and the criteria of fold change≥1.5 and p value<0.05, 380 DEGPs (corresponding to 153 intact N-glycoproteins) were found along with 293 down-regulated and 87 up-regulated.
34074422	5	44	theme	spectrum-level	1015:1028	arg1	%					1035:1035	spectrum-level FDR≤1%	1015:1035	spectrum-level FDR≤1%	1015:1035	With control of spectrum-level FDR≤1%, 5515 intact N-glycopeptides were identified (1737 N-glycosites, 1705 peptide backbones and 1516 intact N-glycoproteins; 181 putative N-glycan linkages and 68 monosaccharide compositions).
34074422	8	45	theme	intact	1631:1636	arg1	N-glycoproteins					1638:1652	these 153 intact N-glycoproteins	1621:1652	these 153 intact N-glycoproteins	1621:1652	For these 153 intact N-glycoproteins, the molecular functions and biological processes of were comprehensively discussed, and side-to-side comparison of differential expression results with other method were also made.
34074422	6	46	gly	N-glycopeptide	1244:1257	arg2	N-glycopeptide					1244:1257	5515 intact N-glycopeptide IDs	1232:1261	5515 intact N-glycopeptide IDs	1232:1261	Among 5515 intact N-glycopeptide IDs, 3864 were identified with glycoform score≥1, i.e., one or more structure-diagnostic fragment ions were observed to distinguish sequence isomers.
34074422	3	47	theme	differentiation	444:458	arg1	potential					413:421	the remarkable potential	398:421	the remarkable potential of self-renewing and differentiation which leads to drug resistance and metastasis	398:504	Cancer stem cells (CSCs) have the remarkable potential of self-renewing and differentiation which leads to drug resistance and metastasis.
34074422	5	48	theme	%	1035:1035	arg1	control					1004:1010	control	1004:1010	control of spectrum-level FDR≤1%	1004:1035	With control of spectrum-level FDR≤1%, 5515 intact N-glycopeptides were identified (1737 N-glycosites, 1705 peptide backbones and 1516 intact N-glycoproteins; 181 putative N-glycan linkages and 68 monosaccharide compositions).
34074422	2	49	theme	high	352:355	arg1	mortality					357:365	high mortality	352:365	high mortality	352:365	N-glycoproteomics studies have shown that N-glycosylation alteration of tumor cells is the key player of cancer progression, multidrug resistance (MDR) and high mortality.
34074422	5	50	gly	1737 N-glycosites	1083:1099	arg2	1737 N-glycosites					1083:1099	1737 N-glycosites	1083:1099	1737 N-glycosites	1083:1099	With control of spectrum-level FDR≤1%, 5515 intact N-glycopeptides were identified (1737 N-glycosites, 1705 peptide backbones and 1516 intact N-glycoproteins; 181 putative N-glycan linkages and 68 monosaccharide compositions).
34074422	4	51	theme	stem	605:608	arg1	cells					610:614	adriamycin-resistant breast cancer stem cells	570:614	adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs)	570:654	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	7	52	theme	p	1485:1485	arg1	value<0.05					1487:1496	p value<0.05	1485:1496	p value<0.05	1485:1496	With the three technical replicates and the criteria of fold change≥1.5 and p value<0.05, 380 DEGPs (corresponding to 153 intact N-glycoproteins) were found along with 293 down-regulated and 87 up-regulated.
34074422	5	53	dep	identified	1071:1080	arg1	N-glycoproteins					1141:1155	1516 intact N-glycoproteins	1129:1155	1516 intact N-glycoproteins	1129:1155	With control of spectrum-level FDR≤1%, 5515 intact N-glycopeptides were identified (1737 N-glycosites, 1705 peptide backbones and 1516 intact N-glycoproteins; 181 putative N-glycan linkages and 68 monosaccharide compositions).
34074422	5	53	dep	identified	1071:1080	arg1	1737 N-glycosites					1083:1099	1737 N-glycosites	1083:1099	1737 N-glycosites	1083:1099	With control of spectrum-level FDR≤1%, 5515 intact N-glycopeptides were identified (1737 N-glycosites, 1705 peptide backbones and 1516 intact N-glycoproteins; 181 putative N-glycan linkages and 68 monosaccharide compositions).
34074422	5	53	dep	identified	1071:1080	arg1	backbones					1115:1123	1705 peptide backbones	1102:1123	1705 peptide backbones	1102:1123	With control of spectrum-level FDR≤1%, 5515 intact N-glycopeptides were identified (1737 N-glycosites, 1705 peptide backbones and 1516 intact N-glycoproteins; 181 putative N-glycan linkages and 68 monosaccharide compositions).
34074422	2	54	theme	key	287:289	arg1	player					291:296	the key player	283:296	the key player of cancer progression	283:318	N-glycoproteomics studies have shown that N-glycosylation alteration of tumor cells is the key player of cancer progression, multidrug resistance (MDR) and high mortality.
34074422	2	54	theme	key	287:289	arg1	alteration					254:263	N-glycosylation alteration	238:263	N-glycosylation alteration of tumor cells	238:278	N-glycoproteomics studies have shown that N-glycosylation alteration of tumor cells is the key player of cancer progression, multidrug resistance (MDR) and high mortality.
34074422	1	55	theme	malignant	162:170	arg1	diseases					172:179	the most malignant diseases	153:179	the most malignant diseases among females	153:193	Breast cancer is one of the most malignant diseases among females.
34074422	7	56	theme	value<0.05	1487:1496	arg1	criteria					1453:1460	the criteria	1449:1460	the criteria of fold change≥1.5 and p value<0.05	1449:1496	With the three technical replicates and the criteria of fold change≥1.5 and p value<0.05, 380 DEGPs (corresponding to 153 intact N-glycoproteins) were found along with 293 down-regulated and 87 up-regulated.
34074422	7	57	theme	change≥1.5	1470:1479	arg1	criteria					1453:1460	the criteria	1449:1460	the criteria of fold change≥1.5 and p value<0.05	1449:1496	With the three technical replicates and the criteria of fold change≥1.5 and p value<0.05, 380 DEGPs (corresponding to 153 intact N-glycoproteins) were found along with 293 down-regulated and 87 up-regulated.
34074422	6	58	theme	glycoform	1290:1298	arg1	score≥1					1300:1306	glycoform score≥1	1290:1306	glycoform score≥1	1290:1306	Among 5515 intact N-glycopeptide IDs, 3864 were identified with glycoform score≥1, i.e., one or more structure-diagnostic fragment ions were observed to distinguish sequence isomers.
34074422	2	59	theme	multidrug	321:329	arg1	MDR					343:345	MDR	343:345	MDR	343:345	N-glycoproteomics studies have shown that N-glycosylation alteration of tumor cells is the key player of cancer progression, multidrug resistance (MDR) and high mortality.
34074422	2	59	theme	multidrug	321:329	arg1	resistance					331:340	multidrug resistance	321:340	multidrug resistance (MDR)	321:346	N-glycoproteomics studies have shown that N-glycosylation alteration of tumor cells is the key player of cancer progression, multidrug resistance (MDR) and high mortality.
34074422	8	60	theme	differential	1770:1781	arg1	results					1794:1800	differential expression results	1770:1800	differential expression results	1770:1800	For these 153 intact N-glycoproteins, the molecular functions and biological processes of were comprehensively discussed, and side-to-side comparison of differential expression results with other method were also made.
34074422	8	61	dep	functions	1669:1677	arg1	the					1655:1657	the	1655:1657	the	1655:1657	For these 153 intact N-glycoproteins, the molecular functions and biological processes of were comprehensively discussed, and side-to-side comparison of differential expression results with other method were also made.
34074422	4	62	theme	stepped	870:876	arg1	NCE					878:880	stepped NCE	870:880	stepped NCE	870:880	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	2	63	theme	progression	308:318	arg1	player					291:296	the key player	283:296	the key player of cancer progression	283:318	N-glycoproteomics studies have shown that N-glycosylation alteration of tumor cells is the key player of cancer progression, multidrug resistance (MDR) and high mortality.
34074422	2	63	theme	progression	308:318	arg1	MDR					343:345	MDR	343:345	MDR	343:345	N-glycoproteomics studies have shown that N-glycosylation alteration of tumor cells is the key player of cancer progression, multidrug resistance (MDR) and high mortality.
34074422	2	63	theme	progression	308:318	arg1	resistance					331:340	multidrug resistance	321:340	multidrug resistance (MDR)	321:346	N-glycoproteomics studies have shown that N-glycosylation alteration of tumor cells is the key player of cancer progression, multidrug resistance (MDR) and high mortality.
34074422	2	63	theme	progression	308:318	arg1	alteration					254:263	N-glycosylation alteration	238:263	N-glycosylation alteration of tumor cells	238:278	N-glycoproteomics studies have shown that N-glycosylation alteration of tumor cells is the key player of cancer progression, multidrug resistance (MDR) and high mortality.
34074422	2	63	theme	progression	308:318	arg1	mortality					357:365	high mortality	352:365	high mortality	352:365	N-glycoproteomics studies have shown that N-glycosylation alteration of tumor cells is the key player of cancer progression, multidrug resistance (MDR) and high mortality.
34074422	8	64	theme	results	1794:1800	arg1	comparison					1756:1765	side-to-side comparison	1743:1765	side-to-side comparison of differential expression results with other method	1743:1818	For these 153 intact N-glycoproteins, the molecular functions and biological processes of were comprehensively discussed, and side-to-side comparison of differential expression results with other method were also made.
34074422	5	65	gly	N-glycoproteins	1141:1155	arg1	N-glycoproteins					1141:1155	1516 intact N-glycoproteins	1129:1155	1516 intact N-glycoproteins	1129:1155	With control of spectrum-level FDR≤1%, 5515 intact N-glycopeptides were identified (1737 N-glycosites, 1705 peptide backbones and 1516 intact N-glycoproteins; 181 putative N-glycan linkages and 68 monosaccharide compositions).
34074422	5	66	theme	1705	1102:1105	arg1	backbones					1115:1123	1705 peptide backbones	1102:1123	1705 peptide backbones	1102:1123	With control of spectrum-level FDR≤1%, 5515 intact N-glycopeptides were identified (1737 N-glycosites, 1705 peptide backbones and 1516 intact N-glycoproteins; 181 putative N-glycan linkages and 68 monosaccharide compositions).
34074422	8	67	theme	other	1807:1811	arg1	method					1813:1818	other method	1807:1818	other method	1807:1818	For these 153 intact N-glycoproteins, the molecular functions and biological processes of were comprehensively discussed, and side-to-side comparison of differential expression results with other method were also made.
34074422	4	68	theme	intact	909:914	arg1	DEGPs					933:937	DEGPs	933:937	DEGPs	933:937	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	4	68	theme	intact	909:914	arg1	N-glycopeptides					916:930	differentially expressed intact N-glycopeptides	884:930	differentially expressed intact N-glycopeptides (DEGPs)	884:938	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	4	69	theme	stable	724:729	arg1	N-glycopeptides					771:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides	690:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs	690:820	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	6	70	theme	structure-diagnostic	1327:1346	arg1	ions					1357:1360	one or more structure-diagnostic fragment ions	1315:1360	one or more structure-diagnostic fragment ions	1315:1360	Among 5515 intact N-glycopeptide IDs, 3864 were identified with glycoform score≥1, i.e., one or more structure-diagnostic fragment ions were observed to distinguish sequence isomers.
34074422	6	71	theme	N-glycopeptide	1244:1257	arg1	IDs					1259:1261	5515 intact N-glycopeptide IDs	1232:1261	5515 intact N-glycopeptide IDs	1232:1261	Among 5515 intact N-glycopeptide IDs, 3864 were identified with glycoform score≥1, i.e., one or more structure-diagnostic fragment ions were observed to distinguish sequence isomers.
34074422	4	72	theme	diethyl	740:746	arg1	N-glycopeptides					771:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides	690:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs	690:820	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	4	73	gly	N-glycopeptides	771:785	arg1	CSCs					817:820	MCF-7 CSCs	811:820	MCF-7 CSCs	811:820	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	4	73	gly	N-glycopeptides	771:785	arg2	N-glycopeptides					771:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides	690:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs	690:820	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	4	73	gly	N-glycopeptides	771:785	arg1	CSCs					802:805	MCF-7/ADR CSCs	792:805	MCF-7/ADR CSCs	792:805	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	2	74	theme	tumor	268:272	arg1	cells					274:278	tumor cells	268:278	tumor cells	268:278	N-glycoproteomics studies have shown that N-glycosylation alteration of tumor cells is the key player of cancer progression, multidrug resistance (MDR) and high mortality.
34074422	4	75	from	CSCs	817:820	arg1	N-glycopeptides					771:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides	690:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs	690:820	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	4	76	gly	N-glycosylation	551:565	arg1	cells					610:614	adriamycin-resistant breast cancer stem cells	570:614	adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs)	570:654	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	4	77	theme	ZIC-HILIC-enriched	701:718	arg1	N-glycopeptides					771:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides	690:785	1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs	690:820	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	0	78	theme	whole	110:114	arg1	lysate					121:126	the whole cell lysate	106:126	the whole cell lysate	106:126	Putative N-glycoprotein markers of MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate.
34074422	5	79	theme	N-glycan	1171:1178	arg1	linkages					1180:1187	181 putative N-glycan linkages	1158:1187	181 putative N-glycan linkages	1158:1187	With control of spectrum-level FDR≤1%, 5515 intact N-glycopeptides were identified (1737 N-glycosites, 1705 peptide backbones and 1516 intact N-glycoproteins; 181 putative N-glycan linkages and 68 monosaccharide compositions).
34074422	4	80	with	HCD	861:863	arg1	NCE					878:880	stepped NCE	870:880	stepped NCE	870:880	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	8	81	with	comparison	1756:1765	arg1	method					1813:1818	other method	1807:1818	other method	1807:1818	For these 153 intact N-glycoproteins, the molecular functions and biological processes of were comprehensively discussed, and side-to-side comparison of differential expression results with other method were also made.
34074422	0	82	theme	lysate	121:126	arg1	characterization					86:101	N-glycoproteomics characterization	68:101	N-glycoproteomics characterization of the whole cell lysate	68:126	Putative N-glycoprotein markers of MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate.
34074422	4	83	theme	search	975:980	arg1	engine					982:987	search engine	975:987	search engine GPSeeker	975:996	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	4	84	theme	MCF-7/ADR	792:800	arg1	CSCs					802:805	MCF-7/ADR CSCs	792:805	MCF-7/ADR CSCs	792:805	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	3	85	theme	self-renewing	426:438	arg1	potential					413:421	the remarkable potential	398:421	the remarkable potential of self-renewing and differentiation which leads to drug resistance and metastasis	398:504	Cancer stem cells (CSCs) have the remarkable potential of self-renewing and differentiation which leads to drug resistance and metastasis.
34074422	0	86	theme	N-glycoprotein	9:22	arg1	markers					24:30	Putative N-glycoprotein markers	0:30	Putative N-glycoprotein markers of MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate.	0:127	Putative N-glycoprotein markers of MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate.
34074422	4	87	theme	adriamycin-resistant	570:589	arg1	cells					610:614	adriamycin-resistant breast cancer stem cells	570:614	adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs)	570:654	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	3	88	theme	stem	375:378	arg1	cells					380:384	Cancer stem cells	368:384	Cancer stem cells (CSCs)	368:391	Cancer stem cells (CSCs) have the remarkable potential of self-renewing and differentiation which leads to drug resistance and metastasis.
34074422	3	88	theme	stem	375:378	arg1	CSCs					387:390	CSCs	387:390	CSCs	387:390	Cancer stem cells (CSCs) have the remarkable potential of self-renewing and differentiation which leads to drug resistance and metastasis.
34074422	4	89	theme	cancer	598:603	arg1	cells					610:614	adriamycin-resistant breast cancer stem cells	570:614	adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs)	570:654	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	4	90	from	N-glycosylation	551:565	arg1	cells					610:614	adriamycin-resistant breast cancer stem cells	570:614	adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs)	570:654	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	4	91	theme	MCF-7	644:648	arg1	CSCs					650:653	MCF-7 CSCs	644:653	MCF-7 CSCs	644:653	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	6	92	theme	sequence	1391:1398	arg1	isomers					1400:1406	sequence isomers	1391:1406	sequence isomers	1391:1406	Among 5515 intact N-glycopeptide IDs, 3864 were identified with glycoform score≥1, i.e., one or more structure-diagnostic fragment ions were observed to distinguish sequence isomers.
34074422	0	93	theme	cells	57:61	arg1	markers					24:30	Putative N-glycoprotein markers	0:30	Putative N-glycoprotein markers of MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate.	0:127	Putative N-glycoprotein markers of MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate.
34074422	5	94	theme	intact	1043:1048	arg1	N-glycopeptides					1050:1064	5515 intact N-glycopeptides	1038:1064	5515 intact N-glycopeptides	1038:1064	With control of spectrum-level FDR≤1%, 5515 intact N-glycopeptides were identified (1737 N-glycosites, 1705 peptide backbones and 1516 intact N-glycoproteins; 181 putative N-glycan linkages and 68 monosaccharide compositions).
34074422	5	95	gly	N-glycopeptides	1050:1064	arg2	N-glycopeptides					1050:1064	5515 intact N-glycopeptides	1038:1064	5515 intact N-glycopeptides	1038:1064	With control of spectrum-level FDR≤1%, 5515 intact N-glycopeptides were identified (1737 N-glycosites, 1705 peptide backbones and 1516 intact N-glycoproteins; 181 putative N-glycan linkages and 68 monosaccharide compositions).
34074422	4	96	dep	cells	610:614	arg1	CSCs					626:629	MCF-7/ADR CSCs	616:629	adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs)	570:654	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34074422	5	97	theme	monosaccharide	1196:1209	arg1	compositions					1211:1222	68 monosaccharide compositions	1193:1222	68 monosaccharide compositions	1193:1222	With control of spectrum-level FDR≤1%, 5515 intact N-glycopeptides were identified (1737 N-glycosites, 1705 peptide backbones and 1516 intact N-glycoproteins; 181 putative N-glycan linkages and 68 monosaccharide compositions).
34074422	8	98	theme	molecular	1659:1667	arg1	functions					1669:1677	molecular functions	1659:1677	molecular functions	1659:1677	For these 153 intact N-glycoproteins, the molecular functions and biological processes of were comprehensively discussed, and side-to-side comparison of differential expression results with other method were also made.
34074422	2	99	theme	N-glycoproteomics	196:212	arg1	studies					214:220	N-glycoproteomics studies	196:220	N-glycoproteomics studies	196:220	N-glycoproteomics studies have shown that N-glycosylation alteration of tumor cells is the key player of cancer progression, multidrug resistance (MDR) and high mortality.
34074422	3	100	theme	drug	475:478	arg1	resistance					480:489	drug resistance	475:489	drug resistance	475:489	Cancer stem cells (CSCs) have the remarkable potential of self-renewing and differentiation which leads to drug resistance and metastasis.
34074422	4	101	theme	MCF-7/ADR	616:624	arg1	CSCs					626:629	MCF-7/ADR CSCs	616:629	adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs)	570:654	To investigate the differentially expressed N-glycosylation in adriamycin-resistant breast cancer stem cells MCF-7/ADR CSCs (relative to MCF-7 CSCs) and find the putative biomarkers, 1:1 paired ZIC-HILIC-enriched and stable isotopic diethyl labelled (SIDE) intact N-glycopeptides from MCF-7/ADR CSCs and MCF-7 CSCs were analyzed with C18-RPLC-ESI-MS/MS (HCD with stepped NCE); differentially expressed intact N-glycopeptides (DEGPs) were identified and quantified via search engine GPSeeker.
34420745	1	0	theme	nutritional	129:139	arg1	Chickpea					89:96	Chickpea	89:96	Chickpea (Cicer arietinum L.) seed	89:122	Chickpea (Cicer arietinum L.) seed is a nutritional food high in starch and protein.
34420745	1	0	theme	nutritional	129:139	arg1	food					141:144	a nutritional food	127:144	a nutritional food high in starch and protein	127:171	Chickpea (Cicer arietinum L.) seed is a nutritional food high in starch and protein.
34420745	4	1	theme	positive	550:557	arg1	correlation					559:569	the positive correlation	546:569	the positive correlation (p < 0.01) between the average molecular size of amylopectin and total protein content	546:656	The initial formation of amylopectin molecules is affected by storage proteins, as suggested by the positive correlation (p < 0.01) between the average molecular size of amylopectin and total protein content.
34420745	4	1	theme	positive	550:557	arg1	p < 0.01					572:579	p < 0.01	572:579	p < 0.01	572:579	The initial formation of amylopectin molecules is affected by storage proteins, as suggested by the positive correlation (p < 0.01) between the average molecular size of amylopectin and total protein content.
34420745	0	2	theme	chickpea	74:81	arg1	seed					83:86	chickpea seed	74:86	chickpea seed	74:86	Starch molecular fine structure is associated with protein composition in chickpea seed.
34420745	2	3	theme	enzymes	317:323	arg1	composition					271:281	the composition	267:281	the composition of storage proteins and metabolic enzymes	267:323	This study aims to find the relationships between the molecular fine structure of starch and the composition of storage proteins and metabolic enzymes, using different chickpea varieties.
34420745	2	3	theme	enzymes	317:323	arg1	structure					243:251	the molecular fine structure	224:251	the molecular fine structure of starch	224:261	This study aims to find the relationships between the molecular fine structure of starch and the composition of storage proteins and metabolic enzymes, using different chickpea varieties.
34420745	6	4	theme	low	925:927	arg1	digestibility					936:948	low starch digestibility	925:948	low starch digestibility	925:948	This study might assist selection of chickpea varieties with desirable qualities, such as low starch digestibility.
34420745	4	5	theme	molecules	487:495	arg1	formation					462:470	The initial formation	450:470	The initial formation of amylopectin molecules	450:495	The initial formation of amylopectin molecules is affected by storage proteins, as suggested by the positive correlation (p < 0.01) between the average molecular size of amylopectin and total protein content.
34420745	2	6	theme	starch	256:261	arg1	composition					271:281	the composition	267:281	the composition of storage proteins and metabolic enzymes	267:323	This study aims to find the relationships between the molecular fine structure of starch and the composition of storage proteins and metabolic enzymes, using different chickpea varieties.
34420745	2	6	theme	starch	256:261	arg1	structure					243:251	the molecular fine structure	224:251	the molecular fine structure of starch	224:261	This study aims to find the relationships between the molecular fine structure of starch and the composition of storage proteins and metabolic enzymes, using different chickpea varieties.
34420745	5	7	theme	seed	691:694	arg1	globulin					696:703	seed globulin	691:703	seed globulin	691:703	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	5	8	theme	globulin	696:703	arg1	globulin					696:703	seed globulin	691:703	seed globulin	691:703	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	5	8	theme	globulin	696:703	arg1	indication					717:726	an indication	714:726	an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000)	714:832	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	5	8	theme	globulin	696:703	arg1	amount					681:686	a higher amount	672:686	a higher amount of seed globulin	672:703	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	5	9	theme	chains	786:791	arg1	globulin					696:703	seed globulin	691:703	seed globulin	691:703	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	5	9	theme	chains	786:791	arg1	indication					717:726	an indication	714:726	an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000)	714:832	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	5	9	theme	chains	786:791	arg1	amount					681:686	a higher amount	672:686	a higher amount of seed globulin	672:703	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	1	10	theme	high	146:149	arg1	Chickpea					89:96	Chickpea	89:96	Chickpea (Cicer arietinum L.) seed	89:122	Chickpea (Cicer arietinum L.) seed is a nutritional food high in starch and protein.
34420745	1	10	theme	high	146:149	arg1	food					141:144	a nutritional food	127:144	a nutritional food high in starch and protein	127:171	Chickpea (Cicer arietinum L.) seed is a nutritional food high in starch and protein.
34420745	6	11	theme	starch	929:934	arg1	digestibility					936:948	low starch digestibility	925:948	low starch digestibility	925:948	This study might assist selection of chickpea varieties with desirable qualities, such as low starch digestibility.
34420745	2	12	theme	fine	238:241	arg1	structure					243:251	the molecular fine structure	224:251	the molecular fine structure of starch	224:261	This study aims to find the relationships between the molecular fine structure of starch and the composition of storage proteins and metabolic enzymes, using different chickpea varieties.
34420745	3	13	theme	biosynthetic	407:418	arg1	enzymes					420:426	starch biosynthetic enzymes	400:426	starch biosynthetic enzymes	400:426	It is found that storage proteins and starch biosynthetic enzymes influence each other.
34420745	0	14	theme	molecular	7:15	arg1	structure					22:30	Starch molecular fine structure	0:30	Starch molecular fine structure	0:30	Starch molecular fine structure is associated with protein composition in chickpea seed.
34420745	4	15	theme	initial	454:460	arg1	formation					462:470	The initial formation	450:470	The initial formation of amylopectin molecules	450:495	The initial formation of amylopectin molecules is affected by storage proteins, as suggested by the positive correlation (p < 0.01) between the average molecular size of amylopectin and total protein content.
34420745	4	16	theme	protein	642:648	arg1	content					650:656	total protein content	636:656	total protein content	636:656	The initial formation of amylopectin molecules is affected by storage proteins, as suggested by the positive correlation (p < 0.01) between the average molecular size of amylopectin and total protein content.
34420745	0	17	theme	Starch	0:5	arg1	structure					22:30	Starch molecular fine structure	0:30	Starch molecular fine structure	0:30	Starch molecular fine structure is associated with protein composition in chickpea seed.
34420745	5	18	theme	polymerization	804:817	arg1	degree					794:799	degree	794:799	degree of polymerization	794:817	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	5	18	theme	polymerization	804:817	arg1	118-2000					824:831	118-2000	824:831	118-2000	824:831	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	5	18	theme	polymerization	804:817	arg1	DP					820:821	DP	820:821	DP	820:821	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	2	19	theme	chickpea	342:349	arg1	varieties					351:359	different chickpea varieties	332:359	different chickpea varieties	332:359	This study aims to find the relationships between the molecular fine structure of starch and the composition of storage proteins and metabolic enzymes, using different chickpea varieties.
34420745	5	20	theme	short	763:767	arg1	chains					786:791	more short - medium amylose chains	758:791	more short - medium amylose chains (degree of polymerization, DP, 118-2000)	758:832	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	4	21	theme	molecular	602:610	arg1	size					612:615	the average molecular size	590:615	the average molecular size of amylopectin	590:630	The initial formation of amylopectin molecules is affected by storage proteins, as suggested by the positive correlation (p < 0.01) between the average molecular size of amylopectin and total protein content.
34420745	0	22	theme	fine	17:20	arg1	structure					22:30	Starch molecular fine structure	0:30	Starch molecular fine structure	0:30	Starch molecular fine structure is associated with protein composition in chickpea seed.
34420745	6	23	theme	desirable	896:904	arg1	qualities					906:914	desirable qualities	896:914	desirable qualities	896:914	This study might assist selection of chickpea varieties with desirable qualities, such as low starch digestibility.
34420745	6	23	theme	desirable	896:904	arg1	digestibility					936:948	low starch digestibility	925:948	low starch digestibility	925:948	This study might assist selection of chickpea varieties with desirable qualities, such as low starch digestibility.
34420745	4	24	theme	average	594:600	arg1	size					612:615	the average molecular size	590:615	the average molecular size of amylopectin	590:630	The initial formation of amylopectin molecules is affected by storage proteins, as suggested by the positive correlation (p < 0.01) between the average molecular size of amylopectin and total protein content.
34420745	5	25	theme	higher	731:736	arg1	content					746:752	higher amylose content	731:752	higher amylose content	731:752	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	5	26	theme	higher	674:679	arg1	globulin					696:703	seed globulin	691:703	seed globulin	691:703	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	5	26	theme	higher	674:679	arg1	indication					717:726	an indication	714:726	an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000)	714:832	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	5	26	theme	higher	674:679	arg1	amount					681:686	a higher amount	672:686	a higher amount of seed globulin	672:703	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	4	27	theme	total	636:640	arg1	content					650:656	total protein content	636:656	total protein content	636:656	The initial formation of amylopectin molecules is affected by storage proteins, as suggested by the positive correlation (p < 0.01) between the average molecular size of amylopectin and total protein content.
34420745	3	28	theme	storage	379:385	arg1	proteins					387:394	storage proteins	379:394	storage proteins	379:394	It is found that storage proteins and starch biosynthetic enzymes influence each other.
34420745	6	29	theme	varieties	881:889	arg1	selection					859:867	selection	859:867	selection of chickpea varieties with desirable qualities, such as low starch digestibility	859:948	This study might assist selection of chickpea varieties with desirable qualities, such as low starch digestibility.
34420745	5	30	theme	amylose	738:744	arg1	content					746:752	higher amylose content	731:752	higher amylose content	731:752	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	0	31	theme	protein	51:57	arg1	composition					59:69	protein composition	51:69	protein composition in chickpea seed	51:86	Starch molecular fine structure is associated with protein composition in chickpea seed.
34420745	6	32	theme	chickpea	872:879	arg1	varieties					881:889	chickpea varieties	872:889	chickpea varieties	872:889	This study might assist selection of chickpea varieties with desirable qualities, such as low starch digestibility.
34420745	0	33	from	composition	59:69	arg1	seed					83:86	chickpea seed	74:86	chickpea seed	74:86	Starch molecular fine structure is associated with protein composition in chickpea seed.
34420745	2	34	theme	metabolic	307:315	arg1	enzymes					317:323	metabolic enzymes	307:323	metabolic enzymes	307:323	This study aims to find the relationships between the molecular fine structure of starch and the composition of storage proteins and metabolic enzymes, using different chickpea varieties.
34420745	6	35	with	selection	859:867	arg1	qualities					906:914	desirable qualities	896:914	desirable qualities	896:914	This study might assist selection of chickpea varieties with desirable qualities, such as low starch digestibility.
34420745	6	35	with	selection	859:867	arg1	digestibility					936:948	low starch digestibility	925:948	low starch digestibility	925:948	This study might assist selection of chickpea varieties with desirable qualities, such as low starch digestibility.
34420745	5	36	from	indication	717:726	arg1	addition					662:669	addition	662:669	addition	662:669	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	5	37	dep	chains	786:791	arg1	degree					794:799	degree	794:799	degree of polymerization	794:817	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	5	37	dep	chains	786:791	arg1	118-2000					824:831	118-2000	824:831	118-2000	824:831	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	5	37	dep	chains	786:791	arg1	DP					820:821	DP	820:821	DP	820:821	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	1	38	from	protein	165:171	arg1	high					146:149	high	146:149	high	146:149	Chickpea (Cicer arietinum L.) seed is a nutritional food high in starch and protein.
34420745	3	39	theme	starch	400:405	arg1	enzymes					420:426	starch biosynthetic enzymes	400:426	starch biosynthetic enzymes	400:426	It is found that storage proteins and starch biosynthetic enzymes influence each other.
34420745	4	40	theme	storage	512:518	arg1	proteins					520:527	storage proteins	512:527	storage proteins	512:527	The initial formation of amylopectin molecules is affected by storage proteins, as suggested by the positive correlation (p < 0.01) between the average molecular size of amylopectin and total protein content.
34420745	2	41	theme	molecular	228:236	arg1	structure					243:251	the molecular fine structure	224:251	the molecular fine structure of starch	224:261	This study aims to find the relationships between the molecular fine structure of starch and the composition of storage proteins and metabolic enzymes, using different chickpea varieties.
34420745	2	42	theme	proteins	294:301	arg1	composition					271:281	the composition	267:281	the composition of storage proteins and metabolic enzymes	267:323	This study aims to find the relationships between the molecular fine structure of starch and the composition of storage proteins and metabolic enzymes, using different chickpea varieties.
34420745	2	42	theme	proteins	294:301	arg1	structure					243:251	the molecular fine structure	224:251	the molecular fine structure of starch	224:261	This study aims to find the relationships between the molecular fine structure of starch and the composition of storage proteins and metabolic enzymes, using different chickpea varieties.
34420745	1	43	from	starch	154:159	arg1	high					146:149	high	146:149	high	146:149	Chickpea (Cicer arietinum L.) seed is a nutritional food high in starch and protein.
34420745	2	44	theme	storage	286:292	arg1	proteins					294:301	storage proteins	286:301	storage proteins	286:301	This study aims to find the relationships between the molecular fine structure of starch and the composition of storage proteins and metabolic enzymes, using different chickpea varieties.
34420745	5	45	theme	amylose	778:784	arg1	chains					786:791	more short - medium amylose chains	758:791	more short - medium amylose chains (degree of polymerization, DP, 118-2000)	758:832	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	2	46	theme	different	332:340	arg1	varieties					351:359	different chickpea varieties	332:359	different chickpea varieties	332:359	This study aims to find the relationships between the molecular fine structure of starch and the composition of storage proteins and metabolic enzymes, using different chickpea varieties.
34420745	4	47	theme	amylopectin	620:630	arg1	size					612:615	the average molecular size	590:615	the average molecular size of amylopectin	590:630	The initial formation of amylopectin molecules is affected by storage proteins, as suggested by the positive correlation (p < 0.01) between the average molecular size of amylopectin and total protein content.
34420745	4	47	theme	amylopectin	620:630	arg1	content					650:656	total protein content	636:656	total protein content	636:656	The initial formation of amylopectin molecules is affected by storage proteins, as suggested by the positive correlation (p < 0.01) between the average molecular size of amylopectin and total protein content.
34420745	5	48	theme	content	746:752	arg1	globulin					696:703	seed globulin	691:703	seed globulin	691:703	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	5	48	theme	content	746:752	arg1	indication					717:726	an indication	714:726	an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000)	714:832	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	5	48	theme	content	746:752	arg1	amount					681:686	a higher amount	672:686	a higher amount of seed globulin	672:703	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	4	49	theme	amylopectin	475:485	arg1	molecules					487:495	amylopectin molecules	475:495	amylopectin molecules	475:495	The initial formation of amylopectin molecules is affected by storage proteins, as suggested by the positive correlation (p < 0.01) between the average molecular size of amylopectin and total protein content.
34420745	5	50	theme	medium	771:776	arg1	chains					786:791	more short - medium amylose chains	758:791	more short - medium amylose chains (degree of polymerization, DP, 118-2000)	758:832	In addition, a higher amount of seed globulin could be an indication of higher amylose content and more short - medium amylose chains (degree of polymerization, DP, 118-2000).
34420745	1	51	from	high	146:149	arg1	starch					154:159	starch	154:159	starch	154:159	Chickpea (Cicer arietinum L.) seed is a nutritional food high in starch and protein.
34420745	1	51	from	high	146:149	arg1	protein					165:171	protein	165:171	protein	165:171	Chickpea (Cicer arietinum L.) seed is a nutritional food high in starch and protein.
34420745	1	52	dep	Cicer	99:103	arg1	L.					115:116	Cicer arietinum L.	99:116	Cicer arietinum L.	99:116	Chickpea (Cicer arietinum L.) seed is a nutritional food high in starch and protein.
34890634	0	0	theme	canning	86:92	arg1	water					105:109	citrus canning processing water	79:109	citrus canning processing water	79:109	Physicochemical, structural and rheological properties of pectin isolated from citrus canning processing water.
34890634	1	1	theme	cellulose	282:290	arg1	column					300:305	cellulose DEAE-52 column	282:305	cellulose DEAE-52 column	282:305	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
34890634	1	2	theme	pectin	150:155	arg1	resource					162:169	the citrus pectin (CP) resource	139:169	the citrus pectin (CP) resource	139:169	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
34890634	0	3	theme	citrus	79:84	arg1	water					105:109	citrus canning processing water	79:109	citrus canning processing water	79:109	Physicochemical, structural and rheological properties of pectin isolated from citrus canning processing water.
34890634	1	4	theme	DEAE-52	292:298	arg1	column					300:305	cellulose DEAE-52 column	282:305	cellulose DEAE-52 column	282:305	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
34890634	4	5	from	composition	633:643	arg1	samples					661:667	these pectin samples	648:667	these pectin samples	648:667	Monosaccharide composition analysis revealed that arabinose was the most abundant composition in these pectin samples.
34890634	9	6	theme	porous	1207:1212	arg1	structures					1214:1223	partly porous structures	1200:1223	partly porous structures	1200:1223	Scanning electron microscopy revealed that CCP, CP1 and CP3 all had irregular sheet-like structures and partly porous structures.
34890634	7	7	theme	α-type	882:887	arg1	linkage					919:925	the main linkage	910:925	the main linkage in the four pectin components	910:955	FT-IR and NMR analysis indicated that α-type glycosidic bonds are the main linkage in the four pectin components.
34890634	7	7	theme	α-type	882:887	arg1	bonds					900:904	α-type glycosidic bonds	882:904	α-type glycosidic bonds	882:904	FT-IR and NMR analysis indicated that α-type glycosidic bonds are the main linkage in the four pectin components.
34890634	3	8	theme	chemical	462:469	arg1	composition					471:481	The chemical composition	458:481	The chemical composition	458:481	The chemical composition, structure and morphology of these pectin components were analyzed.
34890634	11	9	theme	thickening	1434:1443	arg1	agent					1445:1449	food thickening agent	1429:1449	food thickening agent	1429:1449	Due to these properties, the pectin might have potential application in food industry as food thickening agent.
34890634	1	10	dep	polysaccharides	360:374	arg1	CP3					385:387	CP3	385:387	CP3	385:387	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
34890634	1	10	dep	polysaccharides	360:374	arg1	CP1					377:379	CP1	377:379	CP1	377:379	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
34890634	1	10	dep	polysaccharides	360:374	arg1	polysaccharides					360:374	two acidic polysaccharides	349:374	two acidic polysaccharides (CP1 and CP3)	349:388	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
34890634	0	11	theme	processing	94:103	arg1	water					105:109	citrus canning processing water	79:109	citrus canning processing water	79:109	Physicochemical, structural and rheological properties of pectin isolated from citrus canning processing water.
34890634	9	12	theme	Scanning	1096:1103	arg1	microscopy					1114:1123	Scanning electron microscopy	1096:1123	Scanning electron microscopy	1096:1123	Scanning electron microscopy revealed that CCP, CP1 and CP3 all had irregular sheet-like structures and partly porous structures.
34890634	6	13	contain	had	779:781	arg1	CCP					767:769	CCP	767:769	CCP	767:769	Compared with CP3, CCP and CP1 had longer side chains, which are mainly consisted of arabinose.
34890634	6	13	contain	had	779:781	arg2	chains					795:800	longer side chains	783:800	longer side chains	783:800	Compared with CP3, CCP and CP1 had longer side chains, which are mainly consisted of arabinose.
34890634	6	13	contain	had	779:781	arg1	CP1					775:777	CP1	775:777	CP1	775:777	Compared with CP3, CCP and CP1 had longer side chains, which are mainly consisted of arabinose.
34890634	1	14	theme	citrus	221:226	arg1	water					259:263	the citrus fruit canning processing waste water	217:263	the citrus fruit canning processing waste water	217:263	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
34890634	8	15	theme	CP	1082:1083	arg1	fractions					1085:1093	all these CP fractions	1072:1093	all these CP fractions	1072:1093	These CP samples were found to possess different conformation, but no triple-helical conformation was observed in all these CP fractions.
34890634	8	16	theme	CP	964:965	arg1	samples					967:973	These CP samples	958:973	These CP samples	958:973	These CP samples were found to possess different conformation, but no triple-helical conformation was observed in all these CP fractions.
34890634	4	17	theme	composition	566:576	arg1	analysis					578:585	Monosaccharide composition analysis	551:585	Monosaccharide composition analysis	551:585	Monosaccharide composition analysis revealed that arabinose was the most abundant composition in these pectin samples.
34890634	1	18	theme	CP	158:159	arg1	resource					162:169	the citrus pectin (CP) resource	139:169	the citrus pectin (CP) resource	139:169	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
34890634	1	19	theme	neutral	318:324	arg1	CP0					341:343	neutral polysaccharide CP0	318:343	neutral polysaccharide CP0	318:343	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
34890634	2	20	contain	had	395:397	arg2	yield					411:415	the highest yield	399:415	the highest yield	399:415	CP1 had the highest yield among the three fractions, being 44.29%.
34890634	2	20	contain	had	395:397	arg1	CP1					391:393	CP1	391:393	CP1	391:393	CP1 had the highest yield among the three fractions, being 44.29%.
34890634	8	21	located	observed	1060:1067	arg1	fractions					1085:1093	all these CP fractions	1072:1093	all these CP fractions	1072:1093	These CP samples were found to possess different conformation, but no triple-helical conformation was observed in all these CP fractions.
34890634	8	21	located	observed	1060:1067	arg2	conformation					1043:1054	no triple-helical conformation	1025:1054	no triple-helical conformation	1025:1054	These CP samples were found to possess different conformation, but no triple-helical conformation was observed in all these CP fractions.
34890634	8	22	theme	different	997:1005	arg1	conformation					1007:1018	different conformation	997:1018	different conformation	997:1018	These CP samples were found to possess different conformation, but no triple-helical conformation was observed in all these CP fractions.
34890634	1	23	theme	polysaccharide	326:339	arg1	CP0					341:343	neutral polysaccharide CP0	318:343	neutral polysaccharide CP0	318:343	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
34890634	7	24	theme	NMR	854:856	arg1	analysis					858:865	NMR analysis	854:865	NMR analysis	854:865	FT-IR and NMR analysis indicated that α-type glycosidic bonds are the main linkage in the four pectin components.
34890634	0	25	theme	Physicochemical	0:14	arg1	properties					44:53	Physicochemical, structural and rheological properties	0:53	Physicochemical, structural and rheological properties of pectin	0:63	Physicochemical, structural and rheological properties of pectin isolated from citrus canning processing water.
34890634	1	26	theme	fruit	228:232	arg1	water					259:263	the citrus fruit canning processing waste water	217:263	the citrus fruit canning processing waste water	217:263	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
34890634	11	27	theme	potential	1387:1395	arg1	application					1397:1407	potential application	1387:1407	potential application in food industry	1387:1424	Due to these properties, the pectin might have potential application in food industry as food thickening agent.
34890634	1	28	theme	canning	234:240	arg1	water					259:263	the citrus fruit canning processing waste water	217:263	the citrus fruit canning processing waste water	217:263	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
34890634	9	29	contain	had	1160:1162	arg2	structures					1185:1194	irregular sheet-like structures	1164:1194	irregular sheet-like structures	1164:1194	Scanning electron microscopy revealed that CCP, CP1 and CP3 all had irregular sheet-like structures and partly porous structures.
34890634	9	29	contain	had	1160:1162	arg1	CP1					1144:1146	CP1	1144:1146	CP1	1144:1146	Scanning electron microscopy revealed that CCP, CP1 and CP3 all had irregular sheet-like structures and partly porous structures.
34890634	9	29	contain	had	1160:1162	arg1	CP3					1152:1154	CP3	1152:1154	CP3	1152:1154	Scanning electron microscopy revealed that CCP, CP1 and CP3 all had irregular sheet-like structures and partly porous structures.
34890634	9	29	contain	had	1160:1162	arg2	structures					1214:1223	partly porous structures	1200:1223	partly porous structures	1200:1223	Scanning electron microscopy revealed that CCP, CP1 and CP3 all had irregular sheet-like structures and partly porous structures.
34890634	9	29	contain	had	1160:1162	arg1	CCP					1139:1141	CCP	1139:1141	CCP	1139:1141	Scanning electron microscopy revealed that CCP, CP1 and CP3 all had irregular sheet-like structures and partly porous structures.
34890634	9	30	theme	electron	1105:1112	arg1	microscopy					1114:1123	Scanning electron microscopy	1096:1123	Scanning electron microscopy	1096:1123	Scanning electron microscopy revealed that CCP, CP1 and CP3 all had irregular sheet-like structures and partly porous structures.
34890634	0	31	theme	structural	17:26	arg1	properties					44:53	Physicochemical, structural and rheological properties	0:53	Physicochemical, structural and rheological properties of pectin	0:63	Physicochemical, structural and rheological properties of pectin isolated from citrus canning processing water.
34890634	1	32	theme	processing	242:251	arg1	water					259:263	the citrus fruit canning processing waste water	217:263	the citrus fruit canning processing waste water	217:263	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
34890634	6	33	theme	side	790:793	arg1	chains					795:800	longer side chains	783:800	longer side chains	783:800	Compared with CP3, CCP and CP1 had longer side chains, which are mainly consisted of arabinose.
34890634	1	34	theme	crude	176:180	arg1	CCP					197:199	the crude citrus pectin (CCP)	172:200	the crude citrus pectin (CCP)	172:200	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
34890634	5	35	theme	rhamnogalacturonan-I	711:730	arg1	regions					739:745	rhamnogalacturonan-I (RG-I) regions	711:745	rhamnogalacturonan-I (RG-I) regions	711:745	CCP, CP1 and CP3 were mainly composed of rhamnogalacturonan-I (RG-I) regions.
34890634	1	36	theme	waste	253:257	arg1	water					259:263	the citrus fruit canning processing waste water	217:263	the citrus fruit canning processing waste water	217:263	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
34890634	7	37	from	linkage	919:925	arg1	components					946:955	the four pectin components	930:955	the four pectin components	930:955	FT-IR and NMR analysis indicated that α-type glycosidic bonds are the main linkage in the four pectin components.
34890634	6	38	theme	longer	783:788	arg1	chains					795:800	longer side chains	783:800	longer side chains	783:800	Compared with CP3, CCP and CP1 had longer side chains, which are mainly consisted of arabinose.
34890634	3	39	theme	pectin	518:523	arg1	components					525:534	these pectin components	512:534	these pectin components	512:534	The chemical composition, structure and morphology of these pectin components were analyzed.
34890634	1	40	theme	citrus	182:187	arg1	CCP					197:199	the crude citrus pectin (CCP)	172:200	the crude citrus pectin (CCP)	172:200	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
34890634	0	41	theme	rheological	32:42	arg1	properties					44:53	Physicochemical, structural and rheological properties	0:53	Physicochemical, structural and rheological properties of pectin	0:63	Physicochemical, structural and rheological properties of pectin isolated from citrus canning processing water.
34890634	6	42	dep	had	779:781	arg1	consisted					820:828	consisted	820:828	are mainly consisted of arabinose	809:841	Compared with CP3, CCP and CP1 had longer side chains, which are mainly consisted of arabinose.
34890634	2	43	theme	highest	403:409	arg1	yield					411:415	the highest yield	399:415	the highest yield	399:415	CP1 had the highest yield among the three fractions, being 44.29%.
34890634	3	44	theme	components	525:534	arg1	structure					484:492	structure	484:492	structure	484:492	The chemical composition, structure and morphology of these pectin components were analyzed.
34890634	3	44	theme	components	525:534	arg1	composition					471:481	The chemical composition	458:481	The chemical composition	458:481	The chemical composition, structure and morphology of these pectin components were analyzed.
34890634	3	44	theme	components	525:534	arg1	morphology					498:507	morphology	498:507	morphology	498:507	The chemical composition, structure and morphology of these pectin components were analyzed.
34890634	1	45	theme	pectin	189:194	arg1	CCP					197:199	the crude citrus pectin (CCP)	172:200	the crude citrus pectin (CCP)	172:200	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
34890634	0	46	theme	pectin	58:63	arg1	properties					44:53	Physicochemical, structural and rheological properties	0:53	Physicochemical, structural and rheological properties of pectin	0:63	Physicochemical, structural and rheological properties of pectin isolated from citrus canning processing water.
34890634	1	47	theme	acidic	353:358	arg1	CP3					385:387	CP3	385:387	CP3	385:387	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
34890634	1	47	theme	acidic	353:358	arg1	CP1					377:379	CP1	377:379	CP1	377:379	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
34890634	1	47	theme	acidic	353:358	arg1	polysaccharides					360:374	two acidic polysaccharides	349:374	two acidic polysaccharides (CP1 and CP3)	349:388	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
34890634	11	48	theme	food	1429:1432	arg1	agent					1445:1449	food thickening agent	1429:1449	food thickening agent	1429:1449	Due to these properties, the pectin might have potential application in food industry as food thickening agent.
34890634	10	49	theme	good	1318:1321	arg1	viscoelasticity					1323:1337	good viscoelasticity	1318:1337	good viscoelasticity	1318:1337	The four pectin components showed the characteristics of non-Newtonian fluids and possessed good viscoelasticity.
34890634	11	50	from	application	1397:1407	arg1	industry					1417:1424	food industry	1412:1424	food industry	1412:1424	Due to these properties, the pectin might have potential application in food industry as food thickening agent.
34890634	7	51	theme	main	914:917	arg1	linkage					919:925	the main linkage	910:925	the main linkage in the four pectin components	910:955	FT-IR and NMR analysis indicated that α-type glycosidic bonds are the main linkage in the four pectin components.
34890634	7	51	theme	main	914:917	arg1	bonds					900:904	α-type glycosidic bonds	882:904	α-type glycosidic bonds	882:904	FT-IR and NMR analysis indicated that α-type glycosidic bonds are the main linkage in the four pectin components.
34890634	4	52	theme	pectin	654:659	arg1	samples					661:667	these pectin samples	648:667	these pectin samples	648:667	Monosaccharide composition analysis revealed that arabinose was the most abundant composition in these pectin samples.
34890634	2	53	dep	fractions	433:441	arg1	%					455:455	44.29%	450:455	44.29%	450:455	CP1 had the highest yield among the three fractions, being 44.29%.
34890634	10	54	theme	pectin	1235:1240	arg1	components					1242:1251	The four pectin components	1226:1251	The four pectin components	1226:1251	The four pectin components showed the characteristics of non-Newtonian fluids and possessed good viscoelasticity.
34890634	7	55	theme	pectin	939:944	arg1	components					946:955	the four pectin components	930:955	the four pectin components	930:955	FT-IR and NMR analysis indicated that α-type glycosidic bonds are the main linkage in the four pectin components.
34890634	9	56	theme	irregular	1164:1172	arg1	structures					1185:1194	irregular sheet-like structures	1164:1194	irregular sheet-like structures	1164:1194	Scanning electron microscopy revealed that CCP, CP1 and CP3 all had irregular sheet-like structures and partly porous structures.
34890634	10	57	theme	fluids	1297:1302	arg1	characteristics					1264:1278	the characteristics	1260:1278	the characteristics of non-Newtonian fluids	1260:1302	The four pectin components showed the characteristics of non-Newtonian fluids and possessed good viscoelasticity.
34890634	4	58	theme	abundant	624:631	arg1	composition					633:643	the most abundant composition	615:643	the most abundant composition in these pectin samples	615:667	Monosaccharide composition analysis revealed that arabinose was the most abundant composition in these pectin samples.
34890634	4	58	theme	abundant	624:631	arg1	arabinose					601:609	arabinose	601:609	arabinose	601:609	Monosaccharide composition analysis revealed that arabinose was the most abundant composition in these pectin samples.
34890634	8	59	theme	triple-helical	1028:1041	arg1	conformation					1043:1054	no triple-helical conformation	1025:1054	no triple-helical conformation	1025:1054	These CP samples were found to possess different conformation, but no triple-helical conformation was observed in all these CP fractions.
34890634	10	60	contain	possessed	1308:1316	arg2	viscoelasticity					1323:1337	good viscoelasticity	1318:1337	good viscoelasticity	1318:1337	The four pectin components showed the characteristics of non-Newtonian fluids and possessed good viscoelasticity.
34890634	10	60	contain	possessed	1308:1316	arg1	components					1242:1251	The four pectin components	1226:1251	The four pectin components	1226:1251	The four pectin components showed the characteristics of non-Newtonian fluids and possessed good viscoelasticity.
34890634	9	61	theme	sheet-like	1174:1183	arg1	structures					1185:1194	irregular sheet-like structures	1164:1194	irregular sheet-like structures	1164:1194	Scanning electron microscopy revealed that CCP, CP1 and CP3 all had irregular sheet-like structures and partly porous structures.
34890634	10	62	theme	non-Newtonian	1283:1295	arg1	fluids					1297:1302	non-Newtonian fluids	1283:1302	non-Newtonian fluids	1283:1302	The four pectin components showed the characteristics of non-Newtonian fluids and possessed good viscoelasticity.
34890634	8	63	contain	possess	989:995	arg1	samples					967:973	These CP samples	958:973	These CP samples	958:973	These CP samples were found to possess different conformation, but no triple-helical conformation was observed in all these CP fractions.
34890634	8	63	contain	possess	989:995	arg2	conformation					1007:1018	different conformation	997:1018	different conformation	997:1018	These CP samples were found to possess different conformation, but no triple-helical conformation was observed in all these CP fractions.
34890634	11	64	theme	food	1412:1415	arg1	industry					1417:1424	food industry	1412:1424	food industry	1412:1424	Due to these properties, the pectin might have potential application in food industry as food thickening agent.
34890634	4	65	theme	Monosaccharide	551:564	arg1	analysis					578:585	Monosaccharide composition analysis	551:585	Monosaccharide composition analysis	551:585	Monosaccharide composition analysis revealed that arabinose was the most abundant composition in these pectin samples.
34890634	5	66	theme	RG-I	733:736	arg1	regions					739:745	rhamnogalacturonan-I (RG-I) regions	711:745	rhamnogalacturonan-I (RG-I) regions	711:745	CCP, CP1 and CP3 were mainly composed of rhamnogalacturonan-I (RG-I) regions.
34890634	11	67	contain	have	1382:1385	arg2	application					1397:1407	potential application	1387:1407	potential application in food industry	1387:1424	Due to these properties, the pectin might have potential application in food industry as food thickening agent.
34890634	11	67	contain	have	1382:1385	arg1	pectin					1369:1374	the pectin	1365:1374	the pectin	1365:1374	Due to these properties, the pectin might have potential application in food industry as food thickening agent.
34890634	7	68	theme	glycosidic	889:898	arg1	linkage					919:925	the main linkage	910:925	the main linkage in the four pectin components	910:955	FT-IR and NMR analysis indicated that α-type glycosidic bonds are the main linkage in the four pectin components.
34890634	7	68	theme	glycosidic	889:898	arg1	bonds					900:904	α-type glycosidic bonds	882:904	α-type glycosidic bonds	882:904	FT-IR and NMR analysis indicated that α-type glycosidic bonds are the main linkage in the four pectin components.
34890634	1	69	theme	citrus	143:148	arg1	resource					162:169	the citrus pectin (CP) resource	139:169	the citrus pectin (CP) resource	139:169	In order to better utilize the citrus pectin (CP) resource, the crude citrus pectin (CCP), obtained from the citrus fruit canning processing waste water, was purified by cellulose DEAE-52 column, providing neutral polysaccharide CP0 and two acidic polysaccharides (CP1 and CP3).
33255084	7	0	theme	genes	1155:1159	arg1	profile					1144:1150	the expression profile	1129:1150	the expression profile of genes that actively participate in cellular processes such as proliferation, vasculogenesis and apoptosis, which may be modulated by the increase of intra- and extracellular Ca2+ concentration	1129:1346	The results suggest that osteoblast developed a modification in the expression profile of genes that actively participate in cellular processes such as proliferation, vasculogenesis and apoptosis, which may be modulated by the increase of intra- and extracellular Ca2+ concentration.
33255084	1	1	theme	Ca+2	243:246	arg1	concentration					248:260	intracellular and extracellular Ca+2 concentration	211:260	intracellular and extracellular Ca+2 concentration	211:260	The aim of this work was to evaluate the expression profile of genes involved in signaling, intracellular and extracellular Ca+2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs.
33255084	7	2	theme	expression	1133:1142	arg1	profile					1144:1150	the expression profile	1129:1150	the expression profile of genes that actively participate in cellular processes such as proliferation, vasculogenesis and apoptosis, which may be modulated by the increase of intra- and extracellular Ca2+ concentration	1129:1346	The results suggest that osteoblast developed a modification in the expression profile of genes that actively participate in cellular processes such as proliferation, vasculogenesis and apoptosis, which may be modulated by the increase of intra- and extracellular Ca2+ concentration.
33255084	0	3	with	contact	78:84	arg1	scaffolds					108:116	cellulose/MWCNTs scaffolds	91:116	cellulose/MWCNTs scaffolds	91:116	Gene expression profile involved in signaling and apoptosis of osteoblasts in contact with cellulose/MWCNTs scaffolds.
33255084	3	4	theme	interest	493:500	arg1	genes					484:488	genes	484:488	genes of interest	484:500	Osteoblast RNA was extracted for sintering cDNA to amplify genes of interest by PCR; intra- and extracellular calcium (Ca2+) was also quantified.
33255084	0	5	theme	cellulose/MWCNTs	91:106	arg1	scaffolds					108:116	cellulose/MWCNTs scaffolds	91:116	cellulose/MWCNTs scaffolds	91:116	Gene expression profile involved in signaling and apoptosis of osteoblasts in contact with cellulose/MWCNTs scaffolds.
33255084	7	6	theme	extracellular	1315:1327	arg1	concentration					1334:1346	extracellular Ca2+ concentration	1315:1346	extracellular Ca2+ concentration	1315:1346	The results suggest that osteoblast developed a modification in the expression profile of genes that actively participate in cellular processes such as proliferation, vasculogenesis and apoptosis, which may be modulated by the increase of intra- and extracellular Ca2+ concentration.
33255084	0	7	from	apoptosis	50:58	arg1	contact					78:84	contact	78:84	contact with cellulose/MWCNTs scaffolds	78:116	Gene expression profile involved in signaling and apoptosis of osteoblasts in contact with cellulose/MWCNTs scaffolds.
33255084	1	8	with	contact	303:309	arg1	scaffold					318:325	a scaffold	316:325	a scaffold made of a composite of BCN/MWCNTs	316:359	The aim of this work was to evaluate the expression profile of genes involved in signaling, intracellular and extracellular Ca+2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs.
33255084	3	9	theme	extracellular	521:533	arg1	Ca2+					544:547	Ca2+	544:547	Ca2+	544:547	Osteoblast RNA was extracted for sintering cDNA to amplify genes of interest by PCR; intra- and extracellular calcium (Ca2+) was also quantified.
33255084	3	9	theme	extracellular	521:533	arg1	calcium					535:541	extracellular calcium	521:541	extracellular calcium (Ca2+)	521:548	Osteoblast RNA was extracted for sintering cDNA to amplify genes of interest by PCR; intra- and extracellular calcium (Ca2+) was also quantified.
33255084	1	10	theme	apoptosis	266:274	arg1	pathways					276:283	apoptosis pathways	266:283	apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs	266:359	The aim of this work was to evaluate the expression profile of genes involved in signaling, intracellular and extracellular Ca+2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs.
33255084	1	11	theme	BCN/MWCNTs	350:359	arg1	BCN/MWCNTs					350:359	BCN/MWCNTs	350:359	BCN/MWCNTs	350:359	The aim of this work was to evaluate the expression profile of genes involved in signaling, intracellular and extracellular Ca+2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs.
33255084	1	11	theme	BCN/MWCNTs	350:359	arg1	composite					337:345	a composite	335:345	a composite of BCN/MWCNTs	335:359	The aim of this work was to evaluate the expression profile of genes involved in signaling, intracellular and extracellular Ca+2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs.
33255084	3	12	theme	Osteoblast	425:434	arg1	RNA					436:438	Osteoblast RNA	425:438	Osteoblast RNA	425:438	Osteoblast RNA was extracted for sintering cDNA to amplify genes of interest by PCR; intra- and extracellular calcium (Ca2+) was also quantified.
33255084	0	13	from	signaling	36:44	arg1	contact					78:84	contact	78:84	contact with cellulose/MWCNTs scaffolds	78:116	Gene expression profile involved in signaling and apoptosis of osteoblasts in contact with cellulose/MWCNTs scaffolds.
33255084	5	14	theme	VEGFA	747:751	arg1	expression					733:742	The expression	729:742	The expression of VEGFA	729:751	The expression of VEGFA increased, except in the treatment of high concentration of MWCNTs, where remained unchanged.
33255084	1	15	theme	expression	160:169	arg1	profile					171:177	the expression profile	156:177	the expression profile of genes involved in signaling, intracellular and extracellular Ca+2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs	156:359	The aim of this work was to evaluate the expression profile of genes involved in signaling, intracellular and extracellular Ca+2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs.
33255084	0	16	theme	expression	5:14	arg1	profile					16:22	Gene expression profile	0:22	Gene expression profile	0:22	Gene expression profile involved in signaling and apoptosis of osteoblasts in contact with cellulose/MWCNTs scaffolds.
33255084	1	17	from	pathways	276:283	arg1	contact					303:309	contact	303:309	contact with a scaffold made of a composite of BCN/MWCNTs	303:359	The aim of this work was to evaluate the expression profile of genes involved in signaling, intracellular and extracellular Ca+2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs.
33255084	6	18	from	TP53	950:953	arg1	BC					958:959	BC	958:959	BC	958:959	The expression of genes Apaf-1 and Bcl-2/Bax and TP53 increased as compared to the control (except for TP53 in BC and C1/MWCNTs) indicating that cells are responding to the presence of BCN-MWCNTs composites scaffolds.
33255084	6	18	from	TP53	950:953	arg1	C1/MWCNTs					965:973	C1/MWCNTs	965:973	C1/MWCNTs	965:973	The expression of genes Apaf-1 and Bcl-2/Bax and TP53 increased as compared to the control (except for TP53 in BC and C1/MWCNTs) indicating that cells are responding to the presence of BCN-MWCNTs composites scaffolds.
33255084	7	19	theme	Ca2+	1329:1332	arg1	concentration					1334:1346	extracellular Ca2+ concentration	1315:1346	extracellular Ca2+ concentration	1315:1346	The results suggest that osteoblast developed a modification in the expression profile of genes that actively participate in cellular processes such as proliferation, vasculogenesis and apoptosis, which may be modulated by the increase of intra- and extracellular Ca2+ concentration.
33255084	5	20	theme	concentration	796:808	arg1	treatment					778:786	the treatment	774:786	the treatment of high concentration of MWCNTs, where remained unchanged	774:844	The expression of VEGFA increased, except in the treatment of high concentration of MWCNTs, where remained unchanged.
33255084	0	21	theme	Gene	0:3	arg1	profile					16:22	Gene expression profile	0:22	Gene expression profile	0:22	Gene expression profile involved in signaling and apoptosis of osteoblasts in contact with cellulose/MWCNTs scaffolds.
33255084	7	22	theme	concentration	1334:1346	arg1	increase					1292:1299	the increase	1288:1299	the increase of intra- and extracellular Ca2+ concentration	1288:1346	The results suggest that osteoblast developed a modification in the expression profile of genes that actively participate in cellular processes such as proliferation, vasculogenesis and apoptosis, which may be modulated by the increase of intra- and extracellular Ca2+ concentration.
33255084	4	23	theme	NF-KB	691:695	arg1	expression					677:686	only the expression	668:686	only the expression of NF-KB	668:695	Regarding the genes that participate in the regulation paths (MAPK and NF-KB), it was found that only the expression of NF-KB was affected in all treatments.
33255084	1	24	theme	genes	182:186	arg1	profile					171:177	the expression profile	156:177	the expression profile of genes involved in signaling, intracellular and extracellular Ca+2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs	156:359	The aim of this work was to evaluate the expression profile of genes involved in signaling, intracellular and extracellular Ca+2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs.
33255084	1	25	theme	osteoblasts	288:298	arg1	concentration					248:260	intracellular and extracellular Ca+2 concentration	211:260	intracellular and extracellular Ca+2 concentration	211:260	The aim of this work was to evaluate the expression profile of genes involved in signaling, intracellular and extracellular Ca+2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs.
33255084	1	25	theme	osteoblasts	288:298	arg1	pathways					276:283	apoptosis pathways	266:283	apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs	266:359	The aim of this work was to evaluate the expression profile of genes involved in signaling, intracellular and extracellular Ca+2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs.
33255084	1	25	theme	osteoblasts	288:298	arg1	signaling					200:208	signaling	200:208	signaling	200:208	The aim of this work was to evaluate the expression profile of genes involved in signaling, intracellular and extracellular Ca+2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs.
33255084	6	26	dep	genes	865:869	arg1	Apaf-1					871:876	Apaf-1	871:876	Apaf-1	871:876	The expression of genes Apaf-1 and Bcl-2/Bax and TP53 increased as compared to the control (except for TP53 in BC and C1/MWCNTs) indicating that cells are responding to the presence of BCN-MWCNTs composites scaffolds.
33255084	6	26	dep	genes	865:869	arg1	genes					865:869	genes Apaf-1 and Bcl-2/Bax and TP53	865:899	genes Apaf-1 and Bcl-2/Bax and TP53	865:899	The expression of genes Apaf-1 and Bcl-2/Bax and TP53 increased as compared to the control (except for TP53 in BC and C1/MWCNTs) indicating that cells are responding to the presence of BCN-MWCNTs composites scaffolds.
33255084	6	26	dep	genes	865:869	arg1	Bcl-2/Bax					882:890	Bcl-2/Bax	882:890	Bcl-2/Bax	882:890	The expression of genes Apaf-1 and Bcl-2/Bax and TP53 increased as compared to the control (except for TP53 in BC and C1/MWCNTs) indicating that cells are responding to the presence of BCN-MWCNTs composites scaffolds.
33255084	6	27	theme	composites	1043:1052	arg1	scaffolds					1054:1062	BCN-MWCNTs composites scaffolds	1032:1062	BCN-MWCNTs composites scaffolds	1032:1062	The expression of genes Apaf-1 and Bcl-2/Bax and TP53 increased as compared to the control (except for TP53 in BC and C1/MWCNTs) indicating that cells are responding to the presence of BCN-MWCNTs composites scaffolds.
33255084	5	28	theme	MWCNTs	813:818	arg1	concentration					796:808	high concentration	791:808	high concentration	791:808	The expression of VEGFA increased, except in the treatment of high concentration of MWCNTs, where remained unchanged.
33255084	4	29	theme	regulation	615:624	arg1	NF-KB					642:646	NF-KB	642:646	NF-KB	642:646	Regarding the genes that participate in the regulation paths (MAPK and NF-KB), it was found that only the expression of NF-KB was affected in all treatments.
33255084	4	29	theme	regulation	615:624	arg1	MAPK					633:636	MAPK	633:636	MAPK	633:636	Regarding the genes that participate in the regulation paths (MAPK and NF-KB), it was found that only the expression of NF-KB was affected in all treatments.
33255084	4	29	theme	regulation	615:624	arg1	paths					626:630	the regulation paths	611:630	the regulation paths (MAPK and NF-KB)	611:647	Regarding the genes that participate in the regulation paths (MAPK and NF-KB), it was found that only the expression of NF-KB was affected in all treatments.
33255084	1	30	from	concentration	248:260	arg1	contact					303:309	contact	303:309	contact with a scaffold made of a composite of BCN/MWCNTs	303:359	The aim of this work was to evaluate the expression profile of genes involved in signaling, intracellular and extracellular Ca+2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs.
33255084	7	31	theme	intra-	1304:1309	arg1	increase					1292:1299	the increase	1288:1299	the increase of intra- and extracellular Ca2+ concentration	1288:1346	The results suggest that osteoblast developed a modification in the expression profile of genes that actively participate in cellular processes such as proliferation, vasculogenesis and apoptosis, which may be modulated by the increase of intra- and extracellular Ca2+ concentration.
33255084	1	32	from	signaling	200:208	arg1	contact					303:309	contact	303:309	contact with a scaffold made of a composite of BCN/MWCNTs	303:359	The aim of this work was to evaluate the expression profile of genes involved in signaling, intracellular and extracellular Ca+2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs.
33255084	6	33	theme	BCN-MWCNTs	1032:1041	arg1	scaffolds					1054:1062	BCN-MWCNTs composites scaffolds	1032:1062	BCN-MWCNTs composites scaffolds	1032:1062	The expression of genes Apaf-1 and Bcl-2/Bax and TP53 increased as compared to the control (except for TP53 in BC and C1/MWCNTs) indicating that cells are responding to the presence of BCN-MWCNTs composites scaffolds.
33255084	6	34	theme	genes	865:869	arg1	expression					851:860	The expression	847:860	The expression of genes Apaf-1 and Bcl-2/Bax and TP53	847:899	The expression of genes Apaf-1 and Bcl-2/Bax and TP53 increased as compared to the control (except for TP53 in BC and C1/MWCNTs) indicating that cells are responding to the presence of BCN-MWCNTs composites scaffolds.
33255084	6	35	theme	scaffolds	1054:1062	arg1	presence					1020:1027	the presence	1016:1027	the presence of BCN-MWCNTs composites scaffolds	1016:1062	The expression of genes Apaf-1 and Bcl-2/Bax and TP53 increased as compared to the control (except for TP53 in BC and C1/MWCNTs) indicating that cells are responding to the presence of BCN-MWCNTs composites scaffolds.
33255084	7	36	theme	cellular	1190:1197	arg1	apoptosis					1251:1259	apoptosis	1251:1259	apoptosis	1251:1259	The results suggest that osteoblast developed a modification in the expression profile of genes that actively participate in cellular processes such as proliferation, vasculogenesis and apoptosis, which may be modulated by the increase of intra- and extracellular Ca2+ concentration.
33255084	7	36	theme	cellular	1190:1197	arg1	vasculogenesis					1232:1245	vasculogenesis	1232:1245	vasculogenesis	1232:1245	The results suggest that osteoblast developed a modification in the expression profile of genes that actively participate in cellular processes such as proliferation, vasculogenesis and apoptosis, which may be modulated by the increase of intra- and extracellular Ca2+ concentration.
33255084	7	36	theme	cellular	1190:1197	arg1	processes					1199:1207	cellular processes	1190:1207	cellular processes such as proliferation, vasculogenesis and apoptosis, which may be modulated by the increase of intra- and extracellular Ca2+ concentration	1190:1346	The results suggest that osteoblast developed a modification in the expression profile of genes that actively participate in cellular processes such as proliferation, vasculogenesis and apoptosis, which may be modulated by the increase of intra- and extracellular Ca2+ concentration.
33255084	7	36	theme	cellular	1190:1197	arg1	proliferation					1217:1229	proliferation	1217:1229	proliferation	1217:1229	The results suggest that osteoblast developed a modification in the expression profile of genes that actively participate in cellular processes such as proliferation, vasculogenesis and apoptosis, which may be modulated by the increase of intra- and extracellular Ca2+ concentration.
33255084	1	37	theme	intracellular	211:223	arg1	concentration					248:260	intracellular and extracellular Ca+2 concentration	211:260	intracellular and extracellular Ca+2 concentration	211:260	The aim of this work was to evaluate the expression profile of genes involved in signaling, intracellular and extracellular Ca+2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs.
33255084	0	38	theme	osteoblasts	63:73	arg1	signaling					36:44	signaling	36:44	signaling	36:44	Gene expression profile involved in signaling and apoptosis of osteoblasts in contact with cellulose/MWCNTs scaffolds.
33255084	0	38	theme	osteoblasts	63:73	arg1	apoptosis					50:58	apoptosis	50:58	apoptosis	50:58	Gene expression profile involved in signaling and apoptosis of osteoblasts in contact with cellulose/MWCNTs scaffolds.
33255084	1	39	theme	work	135:138	arg1	aim					123:125	The aim	119:125	The aim of this work	119:138	The aim of this work was to evaluate the expression profile of genes involved in signaling, intracellular and extracellular Ca+2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs.
33255084	5	40	theme	high	791:794	arg1	concentration					796:808	high concentration	791:808	high concentration	791:808	The expression of VEGFA increased, except in the treatment of high concentration of MWCNTs, where remained unchanged.
33255084	4	41	dep	paths	626:630	arg1	NF-KB					642:646	NF-KB	642:646	NF-KB	642:646	Regarding the genes that participate in the regulation paths (MAPK and NF-KB), it was found that only the expression of NF-KB was affected in all treatments.
33255084	4	41	dep	paths	626:630	arg1	MAPK					633:636	MAPK	633:636	MAPK	633:636	Regarding the genes that participate in the regulation paths (MAPK and NF-KB), it was found that only the expression of NF-KB was affected in all treatments.
33255084	4	41	dep	paths	626:630	arg1	paths					626:630	the regulation paths	611:630	the regulation paths (MAPK and NF-KB)	611:647	Regarding the genes that participate in the regulation paths (MAPK and NF-KB), it was found that only the expression of NF-KB was affected in all treatments.
33255084	1	42	theme	extracellular	229:241	arg1	concentration					248:260	intracellular and extracellular Ca+2 concentration	211:260	intracellular and extracellular Ca+2 concentration	211:260	The aim of this work was to evaluate the expression profile of genes involved in signaling, intracellular and extracellular Ca+2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs.
33278993	5	0	dep	mono-	810:814	arg1	cations					829:835	cations	829:835	cations	829:835	Both Porphyridium EPSs show a remarkable stability at high temperature and under the effect of mono- and divalent cations, and high salinity cultivation medium, which was evidenced by the rheological properties of the EPS.
33278993	3	1	from	characterization	440:455	arg1	respect					460:466	respect	460:466	respect to their differences in monomer composition, sulfate concentrations and methyl patterns	460:554	purpureum EPS, followed by a thorough rheological characterization in respect to their differences in monomer composition, sulfate concentrations and methyl patterns.
33278993	2	2	theme	EPS	369:371	arg1	L-1					350:352	0.21 g L-1	343:352	0.21 g L-1 of P. sordidum EPS	343:371	For this reason, we produced 0.21 g L-1 of P. sordidum EPS and 0.17 g L-1P.
33278993	2	2	theme	EPS	369:371	arg1	L-1P					384:387	0.17 g L-1P	377:387	0.17 g L-1P	377:387	For this reason, we produced 0.21 g L-1 of P. sordidum EPS and 0.17 g L-1P.
33278993	6	3	theme	carbohydrate	976:987	arg1	polymers					989:996	many carbohydrate polymers	971:996	many carbohydrate polymers	971:996	This feature is not displayed by many carbohydrate polymers, making it possible to enrich current applications in which EPS are used.
33278993	5	4	theme	high	769:772	arg1	temperature					774:784	high temperature	769:784	high temperature	769:784	Both Porphyridium EPSs show a remarkable stability at high temperature and under the effect of mono- and divalent cations, and high salinity cultivation medium, which was evidenced by the rheological properties of the EPS.
33278993	1	5	contain	contain	147:153	arg1	EPSs					134:137	EPSs	134:137	EPSs	134:137	Porphyridium exopolysaccharides (EPSs), which contain sulfate and methyl groups, have a similar potential for use in multiple industrial applications as macroalgae counterparts but lack detailed characterization.
33278993	1	5	contain	contain	147:153	arg2	groups					174:179	methyl groups	167:179	methyl groups	167:179	Porphyridium exopolysaccharides (EPSs), which contain sulfate and methyl groups, have a similar potential for use in multiple industrial applications as macroalgae counterparts but lack detailed characterization.
33278993	1	5	contain	contain	147:153	arg1	exopolysaccharides					114:131	Porphyridium exopolysaccharides	101:131	Porphyridium exopolysaccharides (EPSs)	101:138	Porphyridium exopolysaccharides (EPSs), which contain sulfate and methyl groups, have a similar potential for use in multiple industrial applications as macroalgae counterparts but lack detailed characterization.
33278993	1	5	contain	contain	147:153	arg2	sulfate					155:161	sulfate	155:161	sulfate	155:161	Porphyridium exopolysaccharides (EPSs), which contain sulfate and methyl groups, have a similar potential for use in multiple industrial applications as macroalgae counterparts but lack detailed characterization.
33278993	2	6	theme	sordidum	360:367	arg1	EPS					369:371	P. sordidum EPS	357:371	P. sordidum EPS	357:371	For this reason, we produced 0.21 g L-1 of P. sordidum EPS and 0.17 g L-1P.
33278993	4	7	theme	NaCl	584:587	arg1	effect					574:579	the effect	570:579	the effect of NaCl and CaCl2	570:597	Furthermore, the effect of NaCl and CaCl2 was evaluated, and the effect of high salinity media on the rheological properties of the biopolymers was analyzed.
33278993	5	8	theme	Porphyridium	720:731	arg1	EPSs					733:736	Both Porphyridium EPSs	715:736	Both Porphyridium EPSs	715:736	Both Porphyridium EPSs show a remarkable stability at high temperature and under the effect of mono- and divalent cations, and high salinity cultivation medium, which was evidenced by the rheological properties of the EPS.
33278993	1	9	theme	detailed	287:294	arg1	characterization					296:311	detailed characterization	287:311	detailed characterization	287:311	Porphyridium exopolysaccharides (EPSs), which contain sulfate and methyl groups, have a similar potential for use in multiple industrial applications as macroalgae counterparts but lack detailed characterization.
33278993	1	10	contain	have	182:185	arg1	EPSs					134:137	EPSs	134:137	EPSs	134:137	Porphyridium exopolysaccharides (EPSs), which contain sulfate and methyl groups, have a similar potential for use in multiple industrial applications as macroalgae counterparts but lack detailed characterization.
33278993	1	10	contain	have	182:185	arg1	exopolysaccharides					114:131	Porphyridium exopolysaccharides	101:131	Porphyridium exopolysaccharides (EPSs)	101:138	Porphyridium exopolysaccharides (EPSs), which contain sulfate and methyl groups, have a similar potential for use in multiple industrial applications as macroalgae counterparts but lack detailed characterization.
33278993	1	10	contain	have	182:185	arg2	potential					197:205	a similar potential	187:205	a similar potential for use in multiple industrial applications as macroalgae counterparts	187:276	Porphyridium exopolysaccharides (EPSs), which contain sulfate and methyl groups, have a similar potential for use in multiple industrial applications as macroalgae counterparts but lack detailed characterization.
33278993	4	11	theme	biopolymers	689:699	arg1	properties					671:680	the rheological properties	655:680	the rheological properties of the biopolymers	655:699	Furthermore, the effect of NaCl and CaCl2 was evaluated, and the effect of high salinity media on the rheological properties of the biopolymers was analyzed.
33278993	4	12	theme	CaCl2	593:597	arg1	effect					574:579	the effect	570:579	the effect of NaCl and CaCl2	570:597	Furthermore, the effect of NaCl and CaCl2 was evaluated, and the effect of high salinity media on the rheological properties of the biopolymers was analyzed.
33278993	3	13	theme	monomer	492:498	arg1	composition					500:510	monomer composition	492:510	monomer composition	492:510	purpureum EPS, followed by a thorough rheological characterization in respect to their differences in monomer composition, sulfate concentrations and methyl patterns.
33278993	1	14	theme	Porphyridium	101:112	arg1	EPSs					134:137	EPSs	134:137	EPSs	134:137	Porphyridium exopolysaccharides (EPSs), which contain sulfate and methyl groups, have a similar potential for use in multiple industrial applications as macroalgae counterparts but lack detailed characterization.
33278993	1	14	theme	Porphyridium	101:112	arg1	exopolysaccharides					114:131	Porphyridium exopolysaccharides	101:131	Porphyridium exopolysaccharides (EPSs)	101:138	Porphyridium exopolysaccharides (EPSs), which contain sulfate and methyl groups, have a similar potential for use in multiple industrial applications as macroalgae counterparts but lack detailed characterization.
33278993	5	15	theme	cultivation	856:866	arg1	medium					868:873	high salinity cultivation medium	842:873	high salinity cultivation medium	842:873	Both Porphyridium EPSs show a remarkable stability at high temperature and under the effect of mono- and divalent cations, and high salinity cultivation medium, which was evidenced by the rheological properties of the EPS.
33278993	3	16	theme	rheological	428:438	arg1	characterization					440:455	a thorough rheological characterization	417:455	a thorough rheological characterization in respect to their differences in monomer composition, sulfate concentrations and methyl patterns	417:554	purpureum EPS, followed by a thorough rheological characterization in respect to their differences in monomer composition, sulfate concentrations and methyl patterns.
33278993	3	17	from	differences	477:487	arg1	composition					500:510	monomer composition	492:510	monomer composition	492:510	purpureum EPS, followed by a thorough rheological characterization in respect to their differences in monomer composition, sulfate concentrations and methyl patterns.
33278993	3	17	from	differences	477:487	arg1	concentrations					521:534	sulfate concentrations	513:534	sulfate concentrations	513:534	purpureum EPS, followed by a thorough rheological characterization in respect to their differences in monomer composition, sulfate concentrations and methyl patterns.
33278993	3	17	from	differences	477:487	arg1	patterns					547:554	methyl patterns	540:554	methyl patterns	540:554	purpureum EPS, followed by a thorough rheological characterization in respect to their differences in monomer composition, sulfate concentrations and methyl patterns.
33278993	3	18	theme	purpureum	390:398	arg1	EPS					400:402	purpureum EPS	390:402	purpureum EPS	390:402	purpureum EPS, followed by a thorough rheological characterization in respect to their differences in monomer composition, sulfate concentrations and methyl patterns.
33278993	1	19	theme	similar	189:195	arg1	potential					197:205	a similar potential	187:205	a similar potential for use in multiple industrial applications as macroalgae counterparts	187:276	Porphyridium exopolysaccharides (EPSs), which contain sulfate and methyl groups, have a similar potential for use in multiple industrial applications as macroalgae counterparts but lack detailed characterization.
33278993	4	20	theme	rheological	659:669	arg1	properties					671:680	the rheological properties	655:680	the rheological properties of the biopolymers	655:699	Furthermore, the effect of NaCl and CaCl2 was evaluated, and the effect of high salinity media on the rheological properties of the biopolymers was analyzed.
33278993	0	21	theme	Rheological	0:10	arg1	characterization					12:27	Rheological characterization	0:27	Rheological characterization of Porphyridium sordidum and Porphyridium purpureum exopolysaccharides.	0:99	Rheological characterization of Porphyridium sordidum and Porphyridium purpureum exopolysaccharides.
33278993	5	22	theme	salinity	847:854	arg1	medium					868:873	high salinity cultivation medium	842:873	high salinity cultivation medium	842:873	Both Porphyridium EPSs show a remarkable stability at high temperature and under the effect of mono- and divalent cations, and high salinity cultivation medium, which was evidenced by the rheological properties of the EPS.
33278993	2	23	theme	g	382:382	arg1	L-1P					384:387	0.17 g L-1P	377:387	0.17 g L-1P	377:387	For this reason, we produced 0.21 g L-1 of P. sordidum EPS and 0.17 g L-1P.
33278993	0	24	theme	Porphyridium	32:43	arg1	exopolysaccharides					81:98	Porphyridium sordidum and Porphyridium purpureum exopolysaccharides	32:98	Porphyridium sordidum and Porphyridium purpureum exopolysaccharides	32:98	Rheological characterization of Porphyridium sordidum and Porphyridium purpureum exopolysaccharides.
33278993	5	25	theme	EPS	933:935	arg1	properties					915:924	the rheological properties	899:924	the rheological properties of the EPS	899:935	Both Porphyridium EPSs show a remarkable stability at high temperature and under the effect of mono- and divalent cations, and high salinity cultivation medium, which was evidenced by the rheological properties of the EPS.
33278993	5	26	theme	divalent	820:827	arg1	effect					800:805	the effect	796:805	the effect of mono- and divalent cations	796:835	Both Porphyridium EPSs show a remarkable stability at high temperature and under the effect of mono- and divalent cations, and high salinity cultivation medium, which was evidenced by the rheological properties of the EPS.
33278993	5	26	theme	divalent	820:827	arg1	medium					868:873	high salinity cultivation medium	842:873	high salinity cultivation medium	842:873	Both Porphyridium EPSs show a remarkable stability at high temperature and under the effect of mono- and divalent cations, and high salinity cultivation medium, which was evidenced by the rheological properties of the EPS.
33278993	3	27	theme	methyl	540:545	arg1	patterns					547:554	methyl patterns	540:554	methyl patterns	540:554	purpureum EPS, followed by a thorough rheological characterization in respect to their differences in monomer composition, sulfate concentrations and methyl patterns.
33278993	3	28	theme	sulfate	513:519	arg1	concentrations					521:534	sulfate concentrations	513:534	sulfate concentrations	513:534	purpureum EPS, followed by a thorough rheological characterization in respect to their differences in monomer composition, sulfate concentrations and methyl patterns.
33278993	5	29	theme	mono-	810:814	arg1	effect					800:805	the effect	796:805	the effect of mono- and divalent cations	796:835	Both Porphyridium EPSs show a remarkable stability at high temperature and under the effect of mono- and divalent cations, and high salinity cultivation medium, which was evidenced by the rheological properties of the EPS.
33278993	5	29	theme	mono-	810:814	arg1	medium					868:873	high salinity cultivation medium	842:873	high salinity cultivation medium	842:873	Both Porphyridium EPSs show a remarkable stability at high temperature and under the effect of mono- and divalent cations, and high salinity cultivation medium, which was evidenced by the rheological properties of the EPS.
33278993	4	30	theme	salinity	637:644	arg1	media					646:650	high salinity media	632:650	high salinity media	632:650	Furthermore, the effect of NaCl and CaCl2 was evaluated, and the effect of high salinity media on the rheological properties of the biopolymers was analyzed.
33278993	3	31	theme	thorough	419:426	arg1	characterization					440:455	a thorough rheological characterization	417:455	a thorough rheological characterization in respect to their differences in monomer composition, sulfate concentrations and methyl patterns	417:554	purpureum EPS, followed by a thorough rheological characterization in respect to their differences in monomer composition, sulfate concentrations and methyl patterns.
33278993	1	32	theme	multiple	218:225	arg1	applications					238:249	multiple industrial applications	218:249	multiple industrial applications	218:249	Porphyridium exopolysaccharides (EPSs), which contain sulfate and methyl groups, have a similar potential for use in multiple industrial applications as macroalgae counterparts but lack detailed characterization.
33278993	5	33	theme	high	842:845	arg1	medium					868:873	high salinity cultivation medium	842:873	high salinity cultivation medium	842:873	Both Porphyridium EPSs show a remarkable stability at high temperature and under the effect of mono- and divalent cations, and high salinity cultivation medium, which was evidenced by the rheological properties of the EPS.
33278993	6	34	theme	many	971:974	arg1	polymers					989:996	many carbohydrate polymers	971:996	many carbohydrate polymers	971:996	This feature is not displayed by many carbohydrate polymers, making it possible to enrich current applications in which EPS are used.
33278993	0	35	dep	Porphyridium	58:69	arg1	purpureum					71:79	purpureum	71:79	purpureum	71:79	Rheological characterization of Porphyridium sordidum and Porphyridium purpureum exopolysaccharides.
33278993	1	36	theme	industrial	227:236	arg1	applications					238:249	multiple industrial applications	218:249	multiple industrial applications	218:249	Porphyridium exopolysaccharides (EPSs), which contain sulfate and methyl groups, have a similar potential for use in multiple industrial applications as macroalgae counterparts but lack detailed characterization.
33278993	6	37	used	used	1066:1069	arg2	EPS					1058:1060	EPS	1058:1060	EPS	1058:1060	This feature is not displayed by many carbohydrate polymers, making it possible to enrich current applications in which EPS are used.
33278993	2	38	theme	P.	357:358	arg1	EPS					369:371	P. sordidum EPS	357:371	P. sordidum EPS	357:371	For this reason, we produced 0.21 g L-1 of P. sordidum EPS and 0.17 g L-1P.
33278993	0	39	theme	Porphyridium	58:69	arg1	exopolysaccharides					81:98	Porphyridium sordidum and Porphyridium purpureum exopolysaccharides	32:98	Porphyridium sordidum and Porphyridium purpureum exopolysaccharides	32:98	Rheological characterization of Porphyridium sordidum and Porphyridium purpureum exopolysaccharides.
33278993	1	40	from	use	211:213	arg1	applications					238:249	multiple industrial applications	218:249	multiple industrial applications	218:249	Porphyridium exopolysaccharides (EPSs), which contain sulfate and methyl groups, have a similar potential for use in multiple industrial applications as macroalgae counterparts but lack detailed characterization.
33278993	5	41	from	temperature	774:784	arg1	stability					756:764	a remarkable stability	743:764	a remarkable stability	743:764	Both Porphyridium EPSs show a remarkable stability at high temperature and under the effect of mono- and divalent cations, and high salinity cultivation medium, which was evidenced by the rheological properties of the EPS.
33278993	0	42	dep	Porphyridium	32:43	arg1	sordidum					45:52	sordidum	45:52	sordidum	45:52	Rheological characterization of Porphyridium sordidum and Porphyridium purpureum exopolysaccharides.
33278993	5	43	theme	rheological	903:913	arg1	properties					915:924	the rheological properties	899:924	the rheological properties of the EPS	899:935	Both Porphyridium EPSs show a remarkable stability at high temperature and under the effect of mono- and divalent cations, and high salinity cultivation medium, which was evidenced by the rheological properties of the EPS.
33278993	2	44	theme	g	348:348	arg1	L-1					350:352	0.21 g L-1	343:352	0.21 g L-1 of P. sordidum EPS	343:371	For this reason, we produced 0.21 g L-1 of P. sordidum EPS and 0.17 g L-1P.
33278993	5	45	theme	remarkable	745:754	arg1	stability					756:764	a remarkable stability	743:764	a remarkable stability	743:764	Both Porphyridium EPSs show a remarkable stability at high temperature and under the effect of mono- and divalent cations, and high salinity cultivation medium, which was evidenced by the rheological properties of the EPS.
33278993	4	46	theme	high	632:635	arg1	media					646:650	high salinity media	632:650	high salinity media	632:650	Furthermore, the effect of NaCl and CaCl2 was evaluated, and the effect of high salinity media on the rheological properties of the biopolymers was analyzed.
33278993	6	47	theme	current	1028:1034	arg1	applications					1036:1047	current applications	1028:1047	current applications in which EPS are used	1028:1069	This feature is not displayed by many carbohydrate polymers, making it possible to enrich current applications in which EPS are used.
33278993	1	48	theme	methyl	167:172	arg1	groups					174:179	methyl groups	167:179	methyl groups	167:179	Porphyridium exopolysaccharides (EPSs), which contain sulfate and methyl groups, have a similar potential for use in multiple industrial applications as macroalgae counterparts but lack detailed characterization.
33278993	4	49	theme	media	646:650	arg1	effect					622:627	the effect	618:627	the effect of high salinity media on the rheological properties of the biopolymers	618:699	Furthermore, the effect of NaCl and CaCl2 was evaluated, and the effect of high salinity media on the rheological properties of the biopolymers was analyzed.
33278993	1	50	theme	macroalgae	254:263	arg1	counterparts					265:276	macroalgae counterparts	254:276	macroalgae counterparts	254:276	Porphyridium exopolysaccharides (EPSs), which contain sulfate and methyl groups, have a similar potential for use in multiple industrial applications as macroalgae counterparts but lack detailed characterization.
33278993	0	51	theme	exopolysaccharides	81:98	arg1	characterization					12:27	Rheological characterization	0:27	Rheological characterization of Porphyridium sordidum and Porphyridium purpureum exopolysaccharides.	0:99	Rheological characterization of Porphyridium sordidum and Porphyridium purpureum exopolysaccharides.
33278993	4	52	from	effect	622:627	arg1	properties					671:680	the rheological properties	655:680	the rheological properties of the biopolymers	655:699	Furthermore, the effect of NaCl and CaCl2 was evaluated, and the effect of high salinity media on the rheological properties of the biopolymers was analyzed.
34390990	8	0	theme	composite	1641:1649	arg1	development					1612:1622	the further development	1600:1622	the further development of MXene-polymer composite	1600:1649	In brief, the compatibility issues of MXene were effectively addressed, and its flame-retardancy enhanced greatly via the PCS modification, the bio-based characteristic of which, in turn greatly benefits the further development of MXene-polymer composite.
34390990	6	1	theme	composite	1116:1124	arg1	release					1073:1079	total heat release	1062:1079	total heat release	1062:1079	As only 3 wt% PCS-MXene was introduced, peak heat release rate, total heat release, and total smoke production of the composite decreased by 66.7%, 21.0%, and 27.7%, respectively, compared to the pristine TPU.
34390990	6	1	theme	composite	1116:1124	arg1	rate					1056:1059	peak heat release rate	1038:1059	peak heat release rate	1038:1059	As only 3 wt% PCS-MXene was introduced, peak heat release rate, total heat release, and total smoke production of the composite decreased by 66.7%, 21.0%, and 27.7%, respectively, compared to the pristine TPU.
34390990	6	1	theme	composite	1116:1124	arg1	production					1098:1107	total smoke production	1086:1107	total smoke production of the composite	1086:1124	As only 3 wt% PCS-MXene was introduced, peak heat release rate, total heat release, and total smoke production of the composite decreased by 66.7%, 21.0%, and 27.7%, respectively, compared to the pristine TPU.
34390990	3	2	theme	TPU	538:540	arg1	matrix					543:548	the thermoplastic polyurethane (TPU) matrix	506:548	the thermoplastic polyurethane (TPU) matrix	506:548	The resulting nanohybrid was introduced into the thermoplastic polyurethane (TPU) matrix via solution mixing followed by the hot-pressing method, affording TPU/PCS-MXene nanocomposite.
34390990	8	3	theme	characteristic	1550:1563	arg1	benefits					1591:1598	benefits	1591:1598	benefits	1591:1598	In brief, the compatibility issues of MXene were effectively addressed, and its flame-retardancy enhanced greatly via the PCS modification, the bio-based characteristic of which, in turn greatly benefits the further development of MXene-polymer composite.
34390990	8	4	from	benefits	1591:1598	arg1	turn					1578:1581	turn	1578:1581	turn	1578:1581	In brief, the compatibility issues of MXene were effectively addressed, and its flame-retardancy enhanced greatly via the PCS modification, the bio-based characteristic of which, in turn greatly benefits the further development of MXene-polymer composite.
34390990	8	5	theme	bio-based	1540:1548	arg1	characteristic					1550:1563	the bio-based characteristic	1536:1563	the bio-based characteristic of which, in turn greatly benefits the further development of MXene-polymer composite	1536:1649	In brief, the compatibility issues of MXene were effectively addressed, and its flame-retardancy enhanced greatly via the PCS modification, the bio-based characteristic of which, in turn greatly benefits the further development of MXene-polymer composite.
34390990	8	5	theme	bio-based	1540:1548	arg1	modification					1522:1533	the PCS modification	1514:1533	the PCS modification	1514:1533	In brief, the compatibility issues of MXene were effectively addressed, and its flame-retardancy enhanced greatly via the PCS modification, the bio-based characteristic of which, in turn greatly benefits the further development of MXene-polymer composite.
34390990	4	6	theme	mechanical	735:744	arg1	performance					746:756	mechanical performance	735:756	mechanical performance of the TPU/PCS-MXene nanocomposite	735:791	The resulting nanohybrid exhibited superior compatibility with the TPU matrix, enhancing mechanical performance of the TPU/PCS-MXene nanocomposite compared to the pristine TPU and TPU/MXene nanocomposite.
34390990	6	7	theme	heat	1068:1071	arg1	release					1073:1079	total heat release	1062:1079	total heat release	1062:1079	As only 3 wt% PCS-MXene was introduced, peak heat release rate, total heat release, and total smoke production of the composite decreased by 66.7%, 21.0%, and 27.7%, respectively, compared to the pristine TPU.
34390990	4	8	theme	TPU/MXene	826:834	arg1	nanocomposite					836:848	TPU/MXene nanocomposite	826:848	TPU/MXene nanocomposite	826:848	The resulting nanohybrid exhibited superior compatibility with the TPU matrix, enhancing mechanical performance of the TPU/PCS-MXene nanocomposite compared to the pristine TPU and TPU/MXene nanocomposite.
34390990	1	9	theme	polymer	309:315	arg1	matrix					317:322	polymer matrix	309:322	polymer matrix	309:322	Recently, two-dimensional MXene demonstrated promising advantages to improve the flame-retardant performance of composites; however, its compatibility with polymer matrix is a great concern.
34390990	0	10	theme	smoke	123:127	arg1	properties					141:150	smoke suppression properties	123:150	smoke suppression properties	123:150	Fabrication of thermoplastic polyurethane with functionalized MXene towards high mechanical strength, flame-retardant, and smoke suppression properties.
34390990	4	11	theme	nanocomposite	779:791	arg1	performance					746:756	mechanical performance	735:756	mechanical performance of the TPU/PCS-MXene nanocomposite	735:791	The resulting nanohybrid exhibited superior compatibility with the TPU matrix, enhancing mechanical performance of the TPU/PCS-MXene nanocomposite compared to the pristine TPU and TPU/MXene nanocomposite.
34390990	4	12	theme	superior	681:688	arg1	compatibility					690:702	superior compatibility	681:702	superior compatibility	681:702	The resulting nanohybrid exhibited superior compatibility with the TPU matrix, enhancing mechanical performance of the TPU/PCS-MXene nanocomposite compared to the pristine TPU and TPU/MXene nanocomposite.
34390990	3	13	theme	solution	554:561	arg1	mixing					563:568	solution mixing	554:568	solution mixing followed by the hot-pressing method	554:604	The resulting nanohybrid was introduced into the thermoplastic polyurethane (TPU) matrix via solution mixing followed by the hot-pressing method, affording TPU/PCS-MXene nanocomposite.
34390990	1	14	theme	promising	198:206	arg1	advantages					208:217	promising advantages	198:217	promising advantages	198:217	Recently, two-dimensional MXene demonstrated promising advantages to improve the flame-retardant performance of composites; however, its compatibility with polymer matrix is a great concern.
34390990	6	15	theme	total	1062:1066	arg1	release					1073:1079	total heat release	1062:1079	total heat release	1062:1079	As only 3 wt% PCS-MXene was introduced, peak heat release rate, total heat release, and total smoke production of the composite decreased by 66.7%, 21.0%, and 27.7%, respectively, compared to the pristine TPU.
34390990	6	16	theme	pristine	1194:1201	arg1	TPU					1203:1205	the pristine TPU	1190:1205	the pristine TPU	1190:1205	As only 3 wt% PCS-MXene was introduced, peak heat release rate, total heat release, and total smoke production of the composite decreased by 66.7%, 21.0%, and 27.7%, respectively, compared to the pristine TPU.
34390990	4	17	theme	resulting	650:658	arg1	nanohybrid					660:669	The resulting nanohybrid	646:669	The resulting nanohybrid	646:669	The resulting nanohybrid exhibited superior compatibility with the TPU matrix, enhancing mechanical performance of the TPU/PCS-MXene nanocomposite compared to the pristine TPU and TPU/MXene nanocomposite.
34390990	3	18	theme	resulting	465:473	arg1	nanohybrid					475:484	The resulting nanohybrid	461:484	The resulting nanohybrid	461:484	The resulting nanohybrid was introduced into the thermoplastic polyurethane (TPU) matrix via solution mixing followed by the hot-pressing method, affording TPU/PCS-MXene nanocomposite.
34390990	6	19	theme	heat	1043:1046	arg1	rate					1056:1059	peak heat release rate	1038:1059	peak heat release rate	1038:1059	As only 3 wt% PCS-MXene was introduced, peak heat release rate, total heat release, and total smoke production of the composite decreased by 66.7%, 21.0%, and 27.7%, respectively, compared to the pristine TPU.
34390990	4	20	theme	pristine	809:816	arg1	TPU					818:820	the pristine TPU	805:820	the pristine TPU	805:820	The resulting nanohybrid exhibited superior compatibility with the TPU matrix, enhancing mechanical performance of the TPU/PCS-MXene nanocomposite compared to the pristine TPU and TPU/MXene nanocomposite.
34390990	5	21	theme	TPU/PCS-MXene	895:907	arg1	nanocomposite					909:921	TPU/PCS-MXene nanocomposite	895:921	TPU/PCS-MXene nanocomposite	895:921	Besides, the flame-retardant performance of TPU/PCS-MXene nanocomposite was greatly enhanced, while the smoke emission was effectively suppressed.
34390990	5	22	theme	smoke	955:959	arg1	emission					961:968	the smoke emission	951:968	the smoke emission	951:968	Besides, the flame-retardant performance of TPU/PCS-MXene nanocomposite was greatly enhanced, while the smoke emission was effectively suppressed.
34390990	6	23	dep	PCS-MXene	1012:1020	arg1	%					1010:1010	%	1010:1010	%	1010:1010	As only 3 wt% PCS-MXene was introduced, peak heat release rate, total heat release, and total smoke production of the composite decreased by 66.7%, 21.0%, and 27.7%, respectively, compared to the pristine TPU.
34390990	0	24	theme	polyurethane	29:40	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of thermoplastic polyurethane with functionalized MXene towards high mechanical strength, flame-retardant, and smoke suppression properties.	0:151	Fabrication of thermoplastic polyurethane with functionalized MXene towards high mechanical strength, flame-retardant, and smoke suppression properties.
34390990	8	25	theme	MXene	1434:1438	arg1	issues					1424:1429	the compatibility issues	1406:1429	the compatibility issues of MXene	1406:1438	In brief, the compatibility issues of MXene were effectively addressed, and its flame-retardancy enhanced greatly via the PCS modification, the bio-based characteristic of which, in turn greatly benefits the further development of MXene-polymer composite.
34390990	8	26	theme	compatibility	1410:1422	arg1	issues					1424:1429	the compatibility issues	1406:1429	the compatibility issues of MXene	1406:1438	In brief, the compatibility issues of MXene were effectively addressed, and its flame-retardancy enhanced greatly via the PCS modification, the bio-based characteristic of which, in turn greatly benefits the further development of MXene-polymer composite.
34390990	2	27	theme	PCS-MXene	439:447	arg1	nanohybrid					449:458	the PCS-MXene nanohybrid	435:458	the PCS-MXene nanohybrid	435:458	In this study, MXene was first functionalized with phosphorylated chitosan (PCS) to obtain the PCS-MXene nanohybrid.
34390990	0	28	theme	thermoplastic	15:27	arg1	polyurethane					29:40	thermoplastic polyurethane	15:40	thermoplastic polyurethane	15:40	Fabrication of thermoplastic polyurethane with functionalized MXene towards high mechanical strength, flame-retardant, and smoke suppression properties.
34390990	6	29	theme	release	1048:1054	arg1	rate					1056:1059	peak heat release rate	1038:1059	peak heat release rate	1038:1059	As only 3 wt% PCS-MXene was introduced, peak heat release rate, total heat release, and total smoke production of the composite decreased by 66.7%, 21.0%, and 27.7%, respectively, compared to the pristine TPU.
34390990	0	30	theme	suppression	129:139	arg1	properties					141:150	smoke suppression properties	123:150	smoke suppression properties	123:150	Fabrication of thermoplastic polyurethane with functionalized MXene towards high mechanical strength, flame-retardant, and smoke suppression properties.
34390990	3	31	theme	hot-pressing	586:597	arg1	method					599:604	the hot-pressing method	582:604	the hot-pressing method	582:604	The resulting nanohybrid was introduced into the thermoplastic polyurethane (TPU) matrix via solution mixing followed by the hot-pressing method, affording TPU/PCS-MXene nanocomposite.
34390990	1	32	theme	flame-retardant	234:248	arg1	performance					250:260	the flame-retardant performance	230:260	the flame-retardant performance of composites	230:274	Recently, two-dimensional MXene demonstrated promising advantages to improve the flame-retardant performance of composites; however, its compatibility with polymer matrix is a great concern.
34390990	0	33	theme	functionalized	47:60	arg1	MXene					62:66	functionalized MXene	47:66	functionalized MXene towards high mechanical strength, flame-retardant, and smoke suppression properties	47:150	Fabrication of thermoplastic polyurethane with functionalized MXene towards high mechanical strength, flame-retardant, and smoke suppression properties.
34390990	1	34	theme	great	329:333	arg1	compatibility					290:302	its compatibility	286:302	its compatibility with polymer matrix	286:322	Recently, two-dimensional MXene demonstrated promising advantages to improve the flame-retardant performance of composites; however, its compatibility with polymer matrix is a great concern.
34390990	1	34	theme	great	329:333	arg1	concern					335:341	a great concern	327:341	a great concern	327:341	Recently, two-dimensional MXene demonstrated promising advantages to improve the flame-retardant performance of composites; however, its compatibility with polymer matrix is a great concern.
34390990	7	35	theme	Systematical	1208:1219	arg1	characterization					1221:1236	Systematical characterization	1208:1236	Systematical characterization	1208:1236	Systematical characterization was then carried out to investigate the enhancement mechanism of PCS-MXene, highlighting the crucial role of PCS combined with the catalytic effect of MXene.
34390990	7	36	theme	catalytic	1369:1377	arg1	effect					1379:1384	the catalytic effect	1365:1384	the catalytic effect of MXene	1365:1393	Systematical characterization was then carried out to investigate the enhancement mechanism of PCS-MXene, highlighting the crucial role of PCS combined with the catalytic effect of MXene.
34390990	6	37	theme	peak	1038:1041	arg1	rate					1056:1059	peak heat release rate	1038:1059	peak heat release rate	1038:1059	As only 3 wt% PCS-MXene was introduced, peak heat release rate, total heat release, and total smoke production of the composite decreased by 66.7%, 21.0%, and 27.7%, respectively, compared to the pristine TPU.
34390990	5	38	theme	nanocomposite	909:921	arg1	performance					880:890	the flame-retardant performance	860:890	the flame-retardant performance of TPU/PCS-MXene nanocomposite	860:921	Besides, the flame-retardant performance of TPU/PCS-MXene nanocomposite was greatly enhanced, while the smoke emission was effectively suppressed.
34390990	5	39	theme	flame-retardant	864:878	arg1	performance					880:890	the flame-retardant performance	860:890	the flame-retardant performance of TPU/PCS-MXene nanocomposite	860:921	Besides, the flame-retardant performance of TPU/PCS-MXene nanocomposite was greatly enhanced, while the smoke emission was effectively suppressed.
34390990	8	40	theme	further	1604:1610	arg1	development					1612:1622	the further development	1600:1622	the further development of MXene-polymer composite	1600:1649	In brief, the compatibility issues of MXene were effectively addressed, and its flame-retardancy enhanced greatly via the PCS modification, the bio-based characteristic of which, in turn greatly benefits the further development of MXene-polymer composite.
34390990	3	41	theme	polyurethane	524:535	arg1	matrix					543:548	the thermoplastic polyurethane (TPU) matrix	506:548	the thermoplastic polyurethane (TPU) matrix	506:548	The resulting nanohybrid was introduced into the thermoplastic polyurethane (TPU) matrix via solution mixing followed by the hot-pressing method, affording TPU/PCS-MXene nanocomposite.
34390990	8	42	theme	MXene-polymer	1627:1639	arg1	composite					1641:1649	MXene-polymer composite	1627:1649	MXene-polymer composite	1627:1649	In brief, the compatibility issues of MXene were effectively addressed, and its flame-retardancy enhanced greatly via the PCS modification, the bio-based characteristic of which, in turn greatly benefits the further development of MXene-polymer composite.
34390990	3	43	theme	TPU/PCS-MXene	617:629	arg1	nanocomposite					631:643	TPU/PCS-MXene nanocomposite	617:643	TPU/PCS-MXene nanocomposite	617:643	The resulting nanohybrid was introduced into the thermoplastic polyurethane (TPU) matrix via solution mixing followed by the hot-pressing method, affording TPU/PCS-MXene nanocomposite.
34390990	4	44	theme	TPU/PCS-MXene	765:777	arg1	nanocomposite					779:791	the TPU/PCS-MXene nanocomposite	761:791	the TPU/PCS-MXene nanocomposite	761:791	The resulting nanohybrid exhibited superior compatibility with the TPU matrix, enhancing mechanical performance of the TPU/PCS-MXene nanocomposite compared to the pristine TPU and TPU/MXene nanocomposite.
34390990	7	45	theme	PCS-MXene	1303:1311	arg1	mechanism					1290:1298	the enhancement mechanism	1274:1298	the enhancement mechanism of PCS-MXene	1274:1311	Systematical characterization was then carried out to investigate the enhancement mechanism of PCS-MXene, highlighting the crucial role of PCS combined with the catalytic effect of MXene.
34390990	0	46	with	Fabrication	0:10	arg1	MXene					62:66	functionalized MXene	47:66	functionalized MXene towards high mechanical strength, flame-retardant, and smoke suppression properties	47:150	Fabrication of thermoplastic polyurethane with functionalized MXene towards high mechanical strength, flame-retardant, and smoke suppression properties.
34390990	6	47	dep	%	1010:1010	arg1	3 wt					1006:1009	3 wt	1006:1009	3 wt	1006:1009	As only 3 wt% PCS-MXene was introduced, peak heat release rate, total heat release, and total smoke production of the composite decreased by 66.7%, 21.0%, and 27.7%, respectively, compared to the pristine TPU.
34390990	1	48	theme	composites	265:274	arg1	performance					250:260	the flame-retardant performance	230:260	the flame-retardant performance of composites	230:274	Recently, two-dimensional MXene demonstrated promising advantages to improve the flame-retardant performance of composites; however, its compatibility with polymer matrix is a great concern.
34390990	1	49	with	compatibility	290:302	arg1	matrix					317:322	polymer matrix	309:322	polymer matrix	309:322	Recently, two-dimensional MXene demonstrated promising advantages to improve the flame-retardant performance of composites; however, its compatibility with polymer matrix is a great concern.
34390990	7	50	theme	crucial	1331:1337	arg1	role					1339:1342	the crucial role	1327:1342	the crucial role of PCS combined with the catalytic effect of MXene	1327:1393	Systematical characterization was then carried out to investigate the enhancement mechanism of PCS-MXene, highlighting the crucial role of PCS combined with the catalytic effect of MXene.
34390990	3	51	theme	thermoplastic	510:522	arg1	matrix					543:548	the thermoplastic polyurethane (TPU) matrix	506:548	the thermoplastic polyurethane (TPU) matrix	506:548	The resulting nanohybrid was introduced into the thermoplastic polyurethane (TPU) matrix via solution mixing followed by the hot-pressing method, affording TPU/PCS-MXene nanocomposite.
34390990	6	52	theme	smoke	1092:1096	arg1	production					1098:1107	total smoke production	1086:1107	total smoke production of the composite	1086:1124	As only 3 wt% PCS-MXene was introduced, peak heat release rate, total heat release, and total smoke production of the composite decreased by 66.7%, 21.0%, and 27.7%, respectively, compared to the pristine TPU.
34390990	0	53	theme	mechanical	81:90	arg1	strength					92:99	high mechanical strength	76:99	high mechanical strength	76:99	Fabrication of thermoplastic polyurethane with functionalized MXene towards high mechanical strength, flame-retardant, and smoke suppression properties.
34390990	8	54	theme	PCS	1518:1520	arg1	characteristic					1550:1563	the bio-based characteristic	1536:1563	the bio-based characteristic of which, in turn greatly benefits the further development of MXene-polymer composite	1536:1649	In brief, the compatibility issues of MXene were effectively addressed, and its flame-retardancy enhanced greatly via the PCS modification, the bio-based characteristic of which, in turn greatly benefits the further development of MXene-polymer composite.
34390990	8	54	theme	PCS	1518:1520	arg1	modification					1522:1533	the PCS modification	1514:1533	the PCS modification	1514:1533	In brief, the compatibility issues of MXene were effectively addressed, and its flame-retardancy enhanced greatly via the PCS modification, the bio-based characteristic of which, in turn greatly benefits the further development of MXene-polymer composite.
34390990	7	55	theme	PCS	1347:1349	arg1	role					1339:1342	the crucial role	1327:1342	the crucial role of PCS combined with the catalytic effect of MXene	1327:1393	Systematical characterization was then carried out to investigate the enhancement mechanism of PCS-MXene, highlighting the crucial role of PCS combined with the catalytic effect of MXene.
34390990	2	56	theme	phosphorylated	395:408	arg1	PCS					420:422	PCS	420:422	PCS	420:422	In this study, MXene was first functionalized with phosphorylated chitosan (PCS) to obtain the PCS-MXene nanohybrid.
34390990	2	56	theme	phosphorylated	395:408	arg1	chitosan					410:417	phosphorylated chitosan	395:417	phosphorylated chitosan (PCS)	395:423	In this study, MXene was first functionalized with phosphorylated chitosan (PCS) to obtain the PCS-MXene nanohybrid.
34390990	4	57	theme	TPU	713:715	arg1	matrix					717:722	the TPU matrix	709:722	the TPU matrix	709:722	The resulting nanohybrid exhibited superior compatibility with the TPU matrix, enhancing mechanical performance of the TPU/PCS-MXene nanocomposite compared to the pristine TPU and TPU/MXene nanocomposite.
34390990	6	58	theme	total	1086:1090	arg1	production					1098:1107	total smoke production	1086:1107	total smoke production of the composite	1086:1124	As only 3 wt% PCS-MXene was introduced, peak heat release rate, total heat release, and total smoke production of the composite decreased by 66.7%, 21.0%, and 27.7%, respectively, compared to the pristine TPU.
34390990	0	59	theme	high	76:79	arg1	strength					92:99	high mechanical strength	76:99	high mechanical strength	76:99	Fabrication of thermoplastic polyurethane with functionalized MXene towards high mechanical strength, flame-retardant, and smoke suppression properties.
34390990	7	60	theme	MXene	1389:1393	arg1	effect					1379:1384	the catalytic effect	1365:1384	the catalytic effect of MXene	1365:1393	Systematical characterization was then carried out to investigate the enhancement mechanism of PCS-MXene, highlighting the crucial role of PCS combined with the catalytic effect of MXene.
34390990	1	61	theme	two-dimensional	163:177	arg1	MXene					179:183	two-dimensional MXene	163:183	two-dimensional MXene	163:183	Recently, two-dimensional MXene demonstrated promising advantages to improve the flame-retardant performance of composites; however, its compatibility with polymer matrix is a great concern.
34390990	7	62	theme	enhancement	1278:1288	arg1	mechanism					1290:1298	the enhancement mechanism	1274:1298	the enhancement mechanism of PCS-MXene	1274:1311	Systematical characterization was then carried out to investigate the enhancement mechanism of PCS-MXene, highlighting the crucial role of PCS combined with the catalytic effect of MXene.
35181080	2	0	theme	objects	498:504	arg1	fidelity					478:485	the printing fidelity	465:485	the printing fidelity of printed objects	465:504	The results showed that the rheological, viscoelastic, thermal and mechanical properties of the studied hydrocolloids were composition dependent, which was directly linked to the printing fidelity of printed objects.
35181080	2	1	theme	mechanical	357:366	arg1	dependent					425:433	dependent	425:433	dependent	425:433	The results showed that the rheological, viscoelastic, thermal and mechanical properties of the studied hydrocolloids were composition dependent, which was directly linked to the printing fidelity of printed objects.
35181080	2	1	theme	mechanical	357:366	arg1	properties					368:377	thermal and mechanical properties	345:377	thermal and mechanical properties	345:377	The results showed that the rheological, viscoelastic, thermal and mechanical properties of the studied hydrocolloids were composition dependent, which was directly linked to the printing fidelity of printed objects.
35181080	2	2	theme	printed	490:496	arg1	objects					498:504	printed objects	490:504	printed objects	490:504	The results showed that the rheological, viscoelastic, thermal and mechanical properties of the studied hydrocolloids were composition dependent, which was directly linked to the printing fidelity of printed objects.
35181080	1	3	theme	lactose/Whey	165:176	arg1	hydrocolloids					210:222	the lactose/Whey Protein Isolate (WPI) composite hydrocolloids	161:222	the lactose/Whey Protein Isolate (WPI) composite hydrocolloids	161:222	In this study, the lactose/Whey Protein Isolate (WPI) composite hydrocolloids were prepared as an printing material for subsequent 3D printing.
35181080	1	3	theme	lactose/Whey	165:176	arg1	material					253:260	an printing material	241:260	an printing material for subsequent 3D printing	241:287	In this study, the lactose/Whey Protein Isolate (WPI) composite hydrocolloids were prepared as an printing material for subsequent 3D printing.
35181080	7	4	theme	F	1206:1206	arg1	concept					1208:1214	the F concept	1202:1214	the F concept	1202:1214	Therefore, the F concept is a "printable indicator" for dairy by-products that may possess proper printability and provides an alternative approach to make attractive designs for 3D printing foods.
35181080	7	4	theme	F	1206:1206	arg1	"					1241:1241	a "printable indicator"	1219:1241	a "printable indicator" for dairy by-products that may possess proper printability	1219:1300	Therefore, the F concept is a "printable indicator" for dairy by-products that may possess proper printability and provides an alternative approach to make attractive designs for 3D printing foods.
35181080	4	5	theme	printing	777:784	arg1	performance					786:796	printing performance	777:796	printing performance	777:796	This outcome resulted from lactose-derived co-solvation discouraging WPI aggregation during the printing process, which was necessary for improving printing performance.
35181080	1	6	theme	subsequent	266:275	arg1	printing					280:287	subsequent 3D printing	266:287	subsequent 3D printing	266:287	In this study, the lactose/Whey Protein Isolate (WPI) composite hydrocolloids were prepared as an printing material for subsequent 3D printing.
35181080	0	7	theme	printing	110:117	arg1	hydrocolloids					93:105	composite hydrocolloids	83:105	composite hydrocolloids	83:105	Structural characterization and fluidness analysis of lactose/whey protein isolate composite hydrocolloids as printing materials for 3D printing.
35181080	0	7	theme	printing	110:117	arg1	materials					119:127	printing materials	110:127	printing materials for 3D printing	110:143	Structural characterization and fluidness analysis of lactose/whey protein isolate composite hydrocolloids as printing materials for 3D printing.
35181080	3	8	theme	porous	554:559	arg1	microstructure					561:574	a porous microstructure	552:574	a porous microstructure	552:574	The morphology of all printed objects showed a porous microstructure, and their porosity was changed by lactose addition.
35181080	1	9	theme	3D	277:278	arg1	printing					280:287	subsequent 3D printing	266:287	subsequent 3D printing	266:287	In this study, the lactose/Whey Protein Isolate (WPI) composite hydrocolloids were prepared as an printing material for subsequent 3D printing.
35181080	0	10	theme	3D	133:134	arg1	printing					136:143	3D printing	133:143	3D printing	133:143	Structural characterization and fluidness analysis of lactose/whey protein isolate composite hydrocolloids as printing materials for 3D printing.
35181080	5	11	theme	WLF	870:872	arg1	analysis					874:881	material-specific WLF analysis	852:881	material-specific WLF analysis of relaxation times	852:901	Moreover, an innovative Fluidness concept (F), using material-specific WLF analysis of relaxation times, was introduced to quantify the fluidness of lactose/WPI composite hydrocolloids at a certain decay of timescales (from 102 to 10-3 s).
35181080	3	12	theme	objects	537:543	arg1	morphology					511:520	The morphology	507:520	The morphology of all printed objects	507:543	The morphology of all printed objects showed a porous microstructure, and their porosity was changed by lactose addition.
35181080	1	13	theme	Protein	178:184	arg1	hydrocolloids					210:222	the lactose/Whey Protein Isolate (WPI) composite hydrocolloids	161:222	the lactose/Whey Protein Isolate (WPI) composite hydrocolloids	161:222	In this study, the lactose/Whey Protein Isolate (WPI) composite hydrocolloids were prepared as an printing material for subsequent 3D printing.
35181080	1	13	theme	Protein	178:184	arg1	material					253:260	an printing material	241:260	an printing material for subsequent 3D printing	241:287	In this study, the lactose/Whey Protein Isolate (WPI) composite hydrocolloids were prepared as an printing material for subsequent 3D printing.
35181080	7	14	contain	possess	1274:1280	arg2	printability					1289:1300	proper printability	1282:1300	proper printability	1282:1300	Therefore, the F concept is a "printable indicator" for dairy by-products that may possess proper printability and provides an alternative approach to make attractive designs for 3D printing foods.
35181080	7	14	contain	possess	1274:1280	arg1	by-products					1253:1263	dairy by-products	1247:1263	dairy by-products that may possess proper printability	1247:1300	Therefore, the F concept is a "printable indicator" for dairy by-products that may possess proper printability and provides an alternative approach to make attractive designs for 3D printing foods.
35181080	6	15	theme	dimensional	1121:1131	arg1	deviation					1133:1141	dimensional deviation	1121:1141	dimensional deviation	1121:1141	This F concept was superior for the description and control of printing fidelity, dimensional deviation, and textural properties of 3D-printed objects.
35181080	2	16	attach	linked	455:460	arg2	which					436:440	which	436:440	which	436:440	The results showed that the rheological, viscoelastic, thermal and mechanical properties of the studied hydrocolloids were composition dependent, which was directly linked to the printing fidelity of printed objects.
35181080	2	16	attach	linked	455:460	arg1	fidelity					478:485	the printing fidelity	465:485	the printing fidelity of printed objects	465:504	The results showed that the rheological, viscoelastic, thermal and mechanical properties of the studied hydrocolloids were composition dependent, which was directly linked to the printing fidelity of printed objects.
35181080	5	17	theme	hydrocolloids	970:982	arg1	fluidness					935:943	the fluidness	931:943	the fluidness of lactose/WPI composite hydrocolloids	931:982	Moreover, an innovative Fluidness concept (F), using material-specific WLF analysis of relaxation times, was introduced to quantify the fluidness of lactose/WPI composite hydrocolloids at a certain decay of timescales (from 102 to 10-3 s).
35181080	2	18	theme	hydrocolloids	394:406	arg1	dependent					425:433	dependent	425:433	dependent	425:433	The results showed that the rheological, viscoelastic, thermal and mechanical properties of the studied hydrocolloids were composition dependent, which was directly linked to the printing fidelity of printed objects.
35181080	2	18	theme	hydrocolloids	394:406	arg1	properties					368:377	thermal and mechanical properties	345:377	thermal and mechanical properties	345:377	The results showed that the rheological, viscoelastic, thermal and mechanical properties of the studied hydrocolloids were composition dependent, which was directly linked to the printing fidelity of printed objects.
35181080	2	19	theme	rheological	318:328	arg1	dependent					425:433	dependent	425:433	dependent	425:433	The results showed that the rheological, viscoelastic, thermal and mechanical properties of the studied hydrocolloids were composition dependent, which was directly linked to the printing fidelity of printed objects.
35181080	2	19	theme	rheological	318:328	arg1	properties					368:377	thermal and mechanical properties	345:377	thermal and mechanical properties	345:377	The results showed that the rheological, viscoelastic, thermal and mechanical properties of the studied hydrocolloids were composition dependent, which was directly linked to the printing fidelity of printed objects.
35181080	2	20	theme	studied	386:392	arg1	hydrocolloids					394:406	the studied hydrocolloids	382:406	the studied hydrocolloids	382:406	The results showed that the rheological, viscoelastic, thermal and mechanical properties of the studied hydrocolloids were composition dependent, which was directly linked to the printing fidelity of printed objects.
35181080	0	21	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and fluidness analysis of lactose/whey protein isolate composite hydrocolloids as printing materials for 3D printing.
35181080	5	22	theme	relaxation	886:895	arg1	times					897:901	relaxation times	886:901	relaxation times	886:901	Moreover, an innovative Fluidness concept (F), using material-specific WLF analysis of relaxation times, was introduced to quantify the fluidness of lactose/WPI composite hydrocolloids at a certain decay of timescales (from 102 to 10-3 s).
35181080	5	23	theme	times	897:901	arg1	analysis					874:881	material-specific WLF analysis	852:881	material-specific WLF analysis of relaxation times	852:901	Moreover, an innovative Fluidness concept (F), using material-specific WLF analysis of relaxation times, was introduced to quantify the fluidness of lactose/WPI composite hydrocolloids at a certain decay of timescales (from 102 to 10-3 s).
35181080	5	24	dep	10-3	1030:1033	arg1	to					1027:1028	to	1027:1028	to	1027:1028	Moreover, an innovative Fluidness concept (F), using material-specific WLF analysis of relaxation times, was introduced to quantify the fluidness of lactose/WPI composite hydrocolloids at a certain decay of timescales (from 102 to 10-3 s).
35181080	1	25	theme	WPI	195:197	arg1	hydrocolloids					210:222	the lactose/Whey Protein Isolate (WPI) composite hydrocolloids	161:222	the lactose/Whey Protein Isolate (WPI) composite hydrocolloids	161:222	In this study, the lactose/Whey Protein Isolate (WPI) composite hydrocolloids were prepared as an printing material for subsequent 3D printing.
35181080	1	25	theme	WPI	195:197	arg1	material					253:260	an printing material	241:260	an printing material for subsequent 3D printing	241:287	In this study, the lactose/Whey Protein Isolate (WPI) composite hydrocolloids were prepared as an printing material for subsequent 3D printing.
35181080	0	26	theme	fluidness	32:40	arg1	analysis					42:49	fluidness analysis	32:49	fluidness analysis	32:49	Structural characterization and fluidness analysis of lactose/whey protein isolate composite hydrocolloids as printing materials for 3D printing.
35181080	6	27	theme	textural	1148:1155	arg1	properties					1157:1166	textural properties	1148:1166	textural properties of 3D-printed objects	1148:1188	This F concept was superior for the description and control of printing fidelity, dimensional deviation, and textural properties of 3D-printed objects.
35181080	5	28	theme	material-specific	852:868	arg1	analysis					874:881	material-specific WLF analysis	852:881	material-specific WLF analysis of relaxation times	852:901	Moreover, an innovative Fluidness concept (F), using material-specific WLF analysis of relaxation times, was introduced to quantify the fluidness of lactose/WPI composite hydrocolloids at a certain decay of timescales (from 102 to 10-3 s).
35181080	6	29	theme	properties	1157:1166	arg1	control					1091:1097	control	1091:1097	control	1091:1097	This F concept was superior for the description and control of printing fidelity, dimensional deviation, and textural properties of 3D-printed objects.
35181080	6	29	theme	properties	1157:1166	arg1	description					1075:1085	description	1075:1085	description	1075:1085	This F concept was superior for the description and control of printing fidelity, dimensional deviation, and textural properties of 3D-printed objects.
35181080	5	30	theme	timescales	1006:1015	arg1	decay					997:1001	a certain decay	987:1001	a certain decay of timescales (from 102 to 10-3 s)	987:1036	Moreover, an innovative Fluidness concept (F), using material-specific WLF analysis of relaxation times, was introduced to quantify the fluidness of lactose/WPI composite hydrocolloids at a certain decay of timescales (from 102 to 10-3 s).
35181080	4	31	theme	printing	725:732	arg1	process					734:740	the printing process	721:740	the printing process	721:740	This outcome resulted from lactose-derived co-solvation discouraging WPI aggregation during the printing process, which was necessary for improving printing performance.
35181080	4	31	theme	printing	725:732	arg1	necessary					753:761	necessary	753:761	necessary	753:761	This outcome resulted from lactose-derived co-solvation discouraging WPI aggregation during the printing process, which was necessary for improving printing performance.
35181080	1	32	theme	composite	200:208	arg1	hydrocolloids					210:222	the lactose/Whey Protein Isolate (WPI) composite hydrocolloids	161:222	the lactose/Whey Protein Isolate (WPI) composite hydrocolloids	161:222	In this study, the lactose/Whey Protein Isolate (WPI) composite hydrocolloids were prepared as an printing material for subsequent 3D printing.
35181080	1	32	theme	composite	200:208	arg1	material					253:260	an printing material	241:260	an printing material for subsequent 3D printing	241:287	In this study, the lactose/Whey Protein Isolate (WPI) composite hydrocolloids were prepared as an printing material for subsequent 3D printing.
35181080	5	33	theme	innovative	812:821	arg1	concept					833:839	an innovative Fluidness concept	809:839	an innovative Fluidness concept (F)	809:843	Moreover, an innovative Fluidness concept (F), using material-specific WLF analysis of relaxation times, was introduced to quantify the fluidness of lactose/WPI composite hydrocolloids at a certain decay of timescales (from 102 to 10-3 s).
35181080	5	33	theme	innovative	812:821	arg1	F					842:842	F	842:842	F	842:842	Moreover, an innovative Fluidness concept (F), using material-specific WLF analysis of relaxation times, was introduced to quantify the fluidness of lactose/WPI composite hydrocolloids at a certain decay of timescales (from 102 to 10-3 s).
35181080	7	34	theme	proper	1282:1287	arg1	printability					1289:1300	proper printability	1282:1300	proper printability	1282:1300	Therefore, the F concept is a "printable indicator" for dairy by-products that may possess proper printability and provides an alternative approach to make attractive designs for 3D printing foods.
35181080	4	35	theme	WPI	698:700	arg1	aggregation					702:712	WPI aggregation	698:712	WPI aggregation	698:712	This outcome resulted from lactose-derived co-solvation discouraging WPI aggregation during the printing process, which was necessary for improving printing performance.
35181080	4	36	theme	lactose-derived	656:670	arg1	co-solvation					672:683	lactose-derived co-solvation	656:683	lactose-derived co-solvation discouraging WPI aggregation during the printing process, which was necessary for improving printing performance	656:796	This outcome resulted from lactose-derived co-solvation discouraging WPI aggregation during the printing process, which was necessary for improving printing performance.
35181080	5	37	theme	Fluidness	823:831	arg1	concept					833:839	an innovative Fluidness concept	809:839	an innovative Fluidness concept (F)	809:843	Moreover, an innovative Fluidness concept (F), using material-specific WLF analysis of relaxation times, was introduced to quantify the fluidness of lactose/WPI composite hydrocolloids at a certain decay of timescales (from 102 to 10-3 s).
35181080	5	37	theme	Fluidness	823:831	arg1	F					842:842	F	842:842	F	842:842	Moreover, an innovative Fluidness concept (F), using material-specific WLF analysis of relaxation times, was introduced to quantify the fluidness of lactose/WPI composite hydrocolloids at a certain decay of timescales (from 102 to 10-3 s).
35181080	6	38	theme	F	1044:1044	arg1	concept					1046:1052	This F concept	1039:1052	This F concept	1039:1052	This F concept was superior for the description and control of printing fidelity, dimensional deviation, and textural properties of 3D-printed objects.
35181080	2	39	theme	printing	469:476	arg1	fidelity					478:485	the printing fidelity	465:485	the printing fidelity of printed objects	465:504	The results showed that the rheological, viscoelastic, thermal and mechanical properties of the studied hydrocolloids were composition dependent, which was directly linked to the printing fidelity of printed objects.
35181080	7	40	theme	printable	1222:1230	arg1	concept					1208:1214	the F concept	1202:1214	the F concept	1202:1214	Therefore, the F concept is a "printable indicator" for dairy by-products that may possess proper printability and provides an alternative approach to make attractive designs for 3D printing foods.
35181080	7	40	theme	printable	1222:1230	arg1	"					1241:1241	a "printable indicator"	1219:1241	a "printable indicator" for dairy by-products that may possess proper printability	1219:1300	Therefore, the F concept is a "printable indicator" for dairy by-products that may possess proper printability and provides an alternative approach to make attractive designs for 3D printing foods.
35181080	7	41	theme	3D	1370:1371	arg1	foods					1382:1386	3D printing foods	1370:1386	3D printing foods	1370:1386	Therefore, the F concept is a "printable indicator" for dairy by-products that may possess proper printability and provides an alternative approach to make attractive designs for 3D printing foods.
35181080	6	42	theme	fidelity	1111:1118	arg1	control					1091:1097	control	1091:1097	control	1091:1097	This F concept was superior for the description and control of printing fidelity, dimensional deviation, and textural properties of 3D-printed objects.
35181080	6	42	theme	fidelity	1111:1118	arg1	description					1075:1085	description	1075:1085	description	1075:1085	This F concept was superior for the description and control of printing fidelity, dimensional deviation, and textural properties of 3D-printed objects.
35181080	2	43	theme	thermal	345:351	arg1	dependent					425:433	dependent	425:433	dependent	425:433	The results showed that the rheological, viscoelastic, thermal and mechanical properties of the studied hydrocolloids were composition dependent, which was directly linked to the printing fidelity of printed objects.
35181080	2	43	theme	thermal	345:351	arg1	properties					368:377	thermal and mechanical properties	345:377	thermal and mechanical properties	345:377	The results showed that the rheological, viscoelastic, thermal and mechanical properties of the studied hydrocolloids were composition dependent, which was directly linked to the printing fidelity of printed objects.
35181080	0	44	theme	lactose/whey	54:65	arg1	protein					67:73	lactose/whey protein	54:73	lactose/whey protein	54:73	Structural characterization and fluidness analysis of lactose/whey protein isolate composite hydrocolloids as printing materials for 3D printing.
35181080	7	45	theme	alternative	1318:1328	arg1	approach					1330:1337	an alternative approach	1315:1337	an alternative approach to make attractive designs for 3D printing foods	1315:1386	Therefore, the F concept is a "printable indicator" for dairy by-products that may possess proper printability and provides an alternative approach to make attractive designs for 3D printing foods.
35181080	6	46	dep	description	1075:1085	arg1	the					1071:1073	the	1071:1073	the	1071:1073	This F concept was superior for the description and control of printing fidelity, dimensional deviation, and textural properties of 3D-printed objects.
35181080	7	47	theme	printing	1373:1380	arg1	foods					1382:1386	3D printing foods	1370:1386	3D printing foods	1370:1386	Therefore, the F concept is a "printable indicator" for dairy by-products that may possess proper printability and provides an alternative approach to make attractive designs for 3D printing foods.
35181080	6	48	theme	deviation	1133:1141	arg1	control					1091:1097	control	1091:1097	control	1091:1097	This F concept was superior for the description and control of printing fidelity, dimensional deviation, and textural properties of 3D-printed objects.
35181080	6	48	theme	deviation	1133:1141	arg1	description					1075:1085	description	1075:1085	description	1075:1085	This F concept was superior for the description and control of printing fidelity, dimensional deviation, and textural properties of 3D-printed objects.
35181080	7	49	theme	dairy	1247:1251	arg1	by-products					1253:1263	dairy by-products	1247:1263	dairy by-products that may possess proper printability	1247:1300	Therefore, the F concept is a "printable indicator" for dairy by-products that may possess proper printability and provides an alternative approach to make attractive designs for 3D printing foods.
35181080	6	50	theme	objects	1182:1188	arg1	properties					1157:1166	textural properties	1148:1166	textural properties of 3D-printed objects	1148:1188	This F concept was superior for the description and control of printing fidelity, dimensional deviation, and textural properties of 3D-printed objects.
35181080	6	50	theme	objects	1182:1188	arg1	fidelity					1111:1118	printing fidelity	1102:1118	printing fidelity	1102:1118	This F concept was superior for the description and control of printing fidelity, dimensional deviation, and textural properties of 3D-printed objects.
35181080	6	50	theme	objects	1182:1188	arg1	deviation					1133:1141	dimensional deviation	1121:1141	dimensional deviation	1121:1141	This F concept was superior for the description and control of printing fidelity, dimensional deviation, and textural properties of 3D-printed objects.
35181080	4	51	link	lactose-derived	656:670	arg1	co-solvation					672:683	lactose-derived co-solvation	656:683	lactose-derived co-solvation discouraging WPI aggregation during the printing process, which was necessary for improving printing performance	656:796	This outcome resulted from lactose-derived co-solvation discouraging WPI aggregation during the printing process, which was necessary for improving printing performance.
35181080	2	52	theme	viscoelastic	331:342	arg1	dependent					425:433	dependent	425:433	dependent	425:433	The results showed that the rheological, viscoelastic, thermal and mechanical properties of the studied hydrocolloids were composition dependent, which was directly linked to the printing fidelity of printed objects.
35181080	2	52	theme	viscoelastic	331:342	arg1	properties					368:377	thermal and mechanical properties	345:377	thermal and mechanical properties	345:377	The results showed that the rheological, viscoelastic, thermal and mechanical properties of the studied hydrocolloids were composition dependent, which was directly linked to the printing fidelity of printed objects.
35181080	6	53	theme	3D-printed	1171:1180	arg1	objects					1182:1188	3D-printed objects	1171:1188	3D-printed objects	1171:1188	This F concept was superior for the description and control of printing fidelity, dimensional deviation, and textural properties of 3D-printed objects.
35181080	5	54	theme	composite	960:968	arg1	hydrocolloids					970:982	lactose/WPI composite hydrocolloids	948:982	lactose/WPI composite hydrocolloids	948:982	Moreover, an innovative Fluidness concept (F), using material-specific WLF analysis of relaxation times, was introduced to quantify the fluidness of lactose/WPI composite hydrocolloids at a certain decay of timescales (from 102 to 10-3 s).
35181080	5	55	theme	lactose/WPI	948:958	arg1	hydrocolloids					970:982	lactose/WPI composite hydrocolloids	948:982	lactose/WPI composite hydrocolloids	948:982	Moreover, an innovative Fluidness concept (F), using material-specific WLF analysis of relaxation times, was introduced to quantify the fluidness of lactose/WPI composite hydrocolloids at a certain decay of timescales (from 102 to 10-3 s).
35181080	0	56	theme	composite	83:91	arg1	hydrocolloids					93:105	composite hydrocolloids	83:105	composite hydrocolloids	83:105	Structural characterization and fluidness analysis of lactose/whey protein isolate composite hydrocolloids as printing materials for 3D printing.
35181080	0	56	theme	composite	83:91	arg1	materials					119:127	printing materials	110:127	printing materials for 3D printing	110:143	Structural characterization and fluidness analysis of lactose/whey protein isolate composite hydrocolloids as printing materials for 3D printing.
35181080	6	57	theme	printing	1102:1109	arg1	fidelity					1111:1118	printing fidelity	1102:1118	printing fidelity	1102:1118	This F concept was superior for the description and control of printing fidelity, dimensional deviation, and textural properties of 3D-printed objects.
35181080	3	58	theme	lactose	611:617	arg1	addition					619:626	lactose addition	611:626	lactose addition	611:626	The morphology of all printed objects showed a porous microstructure, and their porosity was changed by lactose addition.
35181080	5	59	theme	certain	989:995	arg1	decay					997:1001	a certain decay	987:1001	a certain decay of timescales (from 102 to 10-3 s)	987:1036	Moreover, an innovative Fluidness concept (F), using material-specific WLF analysis of relaxation times, was introduced to quantify the fluidness of lactose/WPI composite hydrocolloids at a certain decay of timescales (from 102 to 10-3 s).
35181080	1	60	theme	Isolate	186:192	arg1	hydrocolloids					210:222	the lactose/Whey Protein Isolate (WPI) composite hydrocolloids	161:222	the lactose/Whey Protein Isolate (WPI) composite hydrocolloids	161:222	In this study, the lactose/Whey Protein Isolate (WPI) composite hydrocolloids were prepared as an printing material for subsequent 3D printing.
35181080	1	60	theme	Isolate	186:192	arg1	material					253:260	an printing material	241:260	an printing material for subsequent 3D printing	241:287	In this study, the lactose/Whey Protein Isolate (WPI) composite hydrocolloids were prepared as an printing material for subsequent 3D printing.
35181080	0	61	theme	protein	67:73	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and fluidness analysis of lactose/whey protein isolate composite hydrocolloids as printing materials for 3D printing.
35181080	0	61	theme	protein	67:73	arg1	analysis					42:49	fluidness analysis	32:49	fluidness analysis	32:49	Structural characterization and fluidness analysis of lactose/whey protein isolate composite hydrocolloids as printing materials for 3D printing.
35181080	7	62	theme	attractive	1347:1356	arg1	designs					1358:1364	attractive designs	1347:1364	attractive designs for 3D printing foods	1347:1386	Therefore, the F concept is a "printable indicator" for dairy by-products that may possess proper printability and provides an alternative approach to make attractive designs for 3D printing foods.
35181080	3	63	theme	printed	529:535	arg1	objects					537:543	all printed objects	525:543	all printed objects	525:543	The morphology of all printed objects showed a porous microstructure, and their porosity was changed by lactose addition.
35181080	7	64	theme	indicator	1232:1240	arg1	concept					1208:1214	the F concept	1202:1214	the F concept	1202:1214	Therefore, the F concept is a "printable indicator" for dairy by-products that may possess proper printability and provides an alternative approach to make attractive designs for 3D printing foods.
35181080	7	64	theme	indicator	1232:1240	arg1	"					1241:1241	a "printable indicator"	1219:1241	a "printable indicator" for dairy by-products that may possess proper printability	1219:1300	Therefore, the F concept is a "printable indicator" for dairy by-products that may possess proper printability and provides an alternative approach to make attractive designs for 3D printing foods.
35181080	1	65	theme	printing	244:251	arg1	hydrocolloids					210:222	the lactose/Whey Protein Isolate (WPI) composite hydrocolloids	161:222	the lactose/Whey Protein Isolate (WPI) composite hydrocolloids	161:222	In this study, the lactose/Whey Protein Isolate (WPI) composite hydrocolloids were prepared as an printing material for subsequent 3D printing.
35181080	1	65	theme	printing	244:251	arg1	material					253:260	an printing material	241:260	an printing material for subsequent 3D printing	241:287	In this study, the lactose/Whey Protein Isolate (WPI) composite hydrocolloids were prepared as an printing material for subsequent 3D printing.
32532397	4	0	theme	hemoperfusion	979:991	arg1	procedure					993:1001	a 3-h simulative hemoperfusion procedure	962:1001	a 3-h simulative hemoperfusion procedure	962:1001	Most importantly, the optimized C2-K1 hydrogels were competent to eliminate 63.3 % of endotoxin in septic blood with a maximum adsorption capacity of 95.0 EU/g during a 3-h simulative hemoperfusion procedure.
32532397	2	1	theme	developed	402:410	arg1	hydrogels					416:424	the developed C-K hydrogels	398:424	the developed C-K hydrogels	398:424	The chemical compositions and morphologies of the developed C-K hydrogels were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and scanning electron microscopy.
32532397	1	2	theme	endotoxin	285:293	arg1	burden					314:319	endotoxin level and bacteria burden	285:319	endotoxin level and bacteria burden in septic blood	285:335	Here, we describe a green approach to fabricate genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood synchronously.
32532397	3	3	theme	chitosan	775:782	arg1	hydrogels					784:792	raw chitosan hydrogels	771:792	raw chitosan hydrogels	771:792	The C-K hydrogels significantly inhibited adverse blood-material interactions such as hemolysis, complement activation, platelet activation and contact activation, and exhibited better anticoagulant properties than raw chitosan hydrogels.
32532397	3	4	theme	adverse	598:604	arg1	hemolysis					642:650	hemolysis	642:650	hemolysis	642:650	The C-K hydrogels significantly inhibited adverse blood-material interactions such as hemolysis, complement activation, platelet activation and contact activation, and exhibited better anticoagulant properties than raw chitosan hydrogels.
32532397	3	4	theme	adverse	598:604	arg1	activation					664:673	complement activation	653:673	complement activation	653:673	The C-K hydrogels significantly inhibited adverse blood-material interactions such as hemolysis, complement activation, platelet activation and contact activation, and exhibited better anticoagulant properties than raw chitosan hydrogels.
32532397	3	4	theme	adverse	598:604	arg1	activation					685:694	platelet activation	676:694	platelet activation	676:694	The C-K hydrogels significantly inhibited adverse blood-material interactions such as hemolysis, complement activation, platelet activation and contact activation, and exhibited better anticoagulant properties than raw chitosan hydrogels.
32532397	3	4	theme	adverse	598:604	arg1	activation					708:717	contact activation	700:717	contact activation	700:717	The C-K hydrogels significantly inhibited adverse blood-material interactions such as hemolysis, complement activation, platelet activation and contact activation, and exhibited better anticoagulant properties than raw chitosan hydrogels.
32532397	3	4	theme	adverse	598:604	arg1	interactions					621:632	adverse blood-material interactions	598:632	adverse blood-material interactions such as hemolysis, complement activation, platelet activation and contact activation	598:717	The C-K hydrogels significantly inhibited adverse blood-material interactions such as hemolysis, complement activation, platelet activation and contact activation, and exhibited better anticoagulant properties than raw chitosan hydrogels.
32532397	3	5	theme	blood-material	606:619	arg1	hemolysis					642:650	hemolysis	642:650	hemolysis	642:650	The C-K hydrogels significantly inhibited adverse blood-material interactions such as hemolysis, complement activation, platelet activation and contact activation, and exhibited better anticoagulant properties than raw chitosan hydrogels.
32532397	3	5	theme	blood-material	606:619	arg1	activation					664:673	complement activation	653:673	complement activation	653:673	The C-K hydrogels significantly inhibited adverse blood-material interactions such as hemolysis, complement activation, platelet activation and contact activation, and exhibited better anticoagulant properties than raw chitosan hydrogels.
32532397	3	5	theme	blood-material	606:619	arg1	activation					685:694	platelet activation	676:694	platelet activation	676:694	The C-K hydrogels significantly inhibited adverse blood-material interactions such as hemolysis, complement activation, platelet activation and contact activation, and exhibited better anticoagulant properties than raw chitosan hydrogels.
32532397	3	5	theme	blood-material	606:619	arg1	activation					708:717	contact activation	700:717	contact activation	700:717	The C-K hydrogels significantly inhibited adverse blood-material interactions such as hemolysis, complement activation, platelet activation and contact activation, and exhibited better anticoagulant properties than raw chitosan hydrogels.
32532397	3	5	theme	blood-material	606:619	arg1	interactions					621:632	adverse blood-material interactions	598:632	adverse blood-material interactions such as hemolysis, complement activation, platelet activation and contact activation	598:717	The C-K hydrogels significantly inhibited adverse blood-material interactions such as hemolysis, complement activation, platelet activation and contact activation, and exhibited better anticoagulant properties than raw chitosan hydrogels.
32532397	4	6	theme	endotoxin	881:889	arg1	endotoxin					881:889	endotoxin	881:889	endotoxin	881:889	Most importantly, the optimized C2-K1 hydrogels were competent to eliminate 63.3 % of endotoxin in septic blood with a maximum adsorption capacity of 95.0 EU/g during a 3-h simulative hemoperfusion procedure.
32532397	4	6	theme	endotoxin	881:889	arg1	%					876:876	63.3 %	871:876	63.3 % of endotoxin	871:889	Most importantly, the optimized C2-K1 hydrogels were competent to eliminate 63.3 % of endotoxin in septic blood with a maximum adsorption capacity of 95.0 EU/g during a 3-h simulative hemoperfusion procedure.
32532397	6	7	theme	severe	1259:1264	arg1	patients					1273:1280	severe septic patients	1259:1280	severe septic patients	1259:1280	It is believed that the C-K hydrogels are promising hemoperfusion sorbents to treat severe septic patients.
32532397	0	8	theme	septic	121:126	arg1	blood					128:132	septic blood	121:132	septic blood	121:132	Anticoagulant chitosan-kappa-carrageenan composite hydrogel sorbent for simultaneous endotoxin and bacteria cleansing in septic blood.
32532397	4	9	theme	simulative	968:977	arg1	procedure					993:1001	a 3-h simulative hemoperfusion procedure	962:1001	a 3-h simulative hemoperfusion procedure	962:1001	Most importantly, the optimized C2-K1 hydrogels were competent to eliminate 63.3 % of endotoxin in septic blood with a maximum adsorption capacity of 95.0 EU/g during a 3-h simulative hemoperfusion procedure.
32532397	4	10	theme	optimized	817:825	arg1	hydrogels					833:841	the optimized C2-K1 hydrogels	813:841	the optimized C2-K1 hydrogels	813:841	Most importantly, the optimized C2-K1 hydrogels were competent to eliminate 63.3 % of endotoxin in septic blood with a maximum adsorption capacity of 95.0 EU/g during a 3-h simulative hemoperfusion procedure.
32532397	4	10	theme	optimized	817:825	arg1	competent					848:856	competent	848:856	competent	848:856	Most importantly, the optimized C2-K1 hydrogels were competent to eliminate 63.3 % of endotoxin in septic blood with a maximum adsorption capacity of 95.0 EU/g during a 3-h simulative hemoperfusion procedure.
32532397	1	11	theme	bacteria	305:312	arg1	burden					314:319	endotoxin level and bacteria burden	285:319	endotoxin level and bacteria burden in septic blood	285:335	Here, we describe a green approach to fabricate genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood synchronously.
32532397	4	12	theme	septic	894:899	arg1	blood					901:905	septic blood	894:905	septic blood	894:905	Most importantly, the optimized C2-K1 hydrogels were competent to eliminate 63.3 % of endotoxin in septic blood with a maximum adsorption capacity of 95.0 EU/g during a 3-h simulative hemoperfusion procedure.
32532397	1	13	theme	genipin	183:189	arg1	hydrogels					240:248	genipin crosslinked chitosan-kappa-carrageenan composite hydrogels	183:248	genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood	183:335	Here, we describe a green approach to fabricate genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood synchronously.
32532397	1	13	theme	genipin	183:189	arg1	hydrogels					255:263	C-K hydrogels	251:263	C-K hydrogels	251:263	Here, we describe a green approach to fabricate genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood synchronously.
32532397	2	14	theme	scanning	526:533	arg1	microscopy					544:553	scanning electron microscopy	526:553	scanning electron microscopy	526:553	The chemical compositions and morphologies of the developed C-K hydrogels were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and scanning electron microscopy.
32532397	4	15	theme	EU/g	950:953	arg1	capacity					933:940	a maximum adsorption capacity	912:940	a maximum adsorption capacity of 95.0 EU/g during a 3-h simulative hemoperfusion procedure	912:1001	Most importantly, the optimized C2-K1 hydrogels were competent to eliminate 63.3 % of endotoxin in septic blood with a maximum adsorption capacity of 95.0 EU/g during a 3-h simulative hemoperfusion procedure.
32532397	6	16	theme	septic	1266:1271	arg1	patients					1273:1280	severe septic patients	1259:1280	severe septic patients	1259:1280	It is believed that the C-K hydrogels are promising hemoperfusion sorbents to treat severe septic patients.
32532397	5	17	theme	C2-K1	1075:1079	arg1	hydrogels					1081:1089	the optimized C2-K1 hydrogels	1061:1089	the optimized C2-K1 hydrogels	1061:1089	Bacteria cleansing experiments further demonstrated that the optimized C2-K1 hydrogels effectively decreased 46.0 % of E. coli and 68.7 % of S. aureus load, respectively.
32532397	1	18	theme	crosslinked	191:201	arg1	hydrogels					240:248	genipin crosslinked chitosan-kappa-carrageenan composite hydrogels	183:248	genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood	183:335	Here, we describe a green approach to fabricate genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood synchronously.
32532397	1	18	theme	crosslinked	191:201	arg1	hydrogels					255:263	C-K hydrogels	251:263	C-K hydrogels	251:263	Here, we describe a green approach to fabricate genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood synchronously.
32532397	5	19	theme	S.	1145:1146	arg1	load					1155:1158	S. aureus load	1145:1158	S. aureus load	1145:1158	Bacteria cleansing experiments further demonstrated that the optimized C2-K1 hydrogels effectively decreased 46.0 % of E. coli and 68.7 % of S. aureus load, respectively.
32532397	1	20	from	burden	314:319	arg1	blood					331:335	septic blood	324:335	septic blood	324:335	Here, we describe a green approach to fabricate genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood synchronously.
32532397	2	21	theme	chemical	356:363	arg1	compositions					365:376	The chemical compositions	352:376	The chemical compositions	352:376	The chemical compositions and morphologies of the developed C-K hydrogels were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and scanning electron microscopy.
32532397	1	22	theme	chitosan-kappa-carrageenan	203:228	arg1	hydrogels					240:248	genipin crosslinked chitosan-kappa-carrageenan composite hydrogels	183:248	genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood	183:335	Here, we describe a green approach to fabricate genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood synchronously.
32532397	1	22	theme	chitosan-kappa-carrageenan	203:228	arg1	hydrogels					255:263	C-K hydrogels	251:263	C-K hydrogels	251:263	Here, we describe a green approach to fabricate genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood synchronously.
32532397	1	23	link	crosslinked	191:201	arg1	hydrogels					240:248	genipin crosslinked chitosan-kappa-carrageenan composite hydrogels	183:248	genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood	183:335	Here, we describe a green approach to fabricate genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood synchronously.
32532397	1	23	link	crosslinked	191:201	arg1	hydrogels					255:263	C-K hydrogels	251:263	C-K hydrogels	251:263	Here, we describe a green approach to fabricate genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood synchronously.
32532397	3	24	theme	platelet	676:683	arg1	activation					685:694	platelet activation	676:694	platelet activation	676:694	The C-K hydrogels significantly inhibited adverse blood-material interactions such as hemolysis, complement activation, platelet activation and contact activation, and exhibited better anticoagulant properties than raw chitosan hydrogels.
32532397	1	25	theme	composite	230:238	arg1	hydrogels					240:248	genipin crosslinked chitosan-kappa-carrageenan composite hydrogels	183:248	genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood	183:335	Here, we describe a green approach to fabricate genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood synchronously.
32532397	1	25	theme	composite	230:238	arg1	hydrogels					255:263	C-K hydrogels	251:263	C-K hydrogels	251:263	Here, we describe a green approach to fabricate genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood synchronously.
32532397	2	26	theme	photoelectron	495:507	arg1	spectroscopy					509:520	X-ray photoelectron spectroscopy	489:520	X-ray photoelectron spectroscopy	489:520	The chemical compositions and morphologies of the developed C-K hydrogels were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and scanning electron microscopy.
32532397	4	27	theme	maximum	914:920	arg1	capacity					933:940	a maximum adsorption capacity	912:940	a maximum adsorption capacity of 95.0 EU/g during a 3-h simulative hemoperfusion procedure	912:1001	Most importantly, the optimized C2-K1 hydrogels were competent to eliminate 63.3 % of endotoxin in septic blood with a maximum adsorption capacity of 95.0 EU/g during a 3-h simulative hemoperfusion procedure.
32532397	5	28	theme	optimized	1065:1073	arg1	hydrogels					1081:1089	the optimized C2-K1 hydrogels	1061:1089	the optimized C2-K1 hydrogels	1061:1089	Bacteria cleansing experiments further demonstrated that the optimized C2-K1 hydrogels effectively decreased 46.0 % of E. coli and 68.7 % of S. aureus load, respectively.
32532397	3	29	theme	complement	653:662	arg1	activation					664:673	complement activation	653:673	complement activation	653:673	The C-K hydrogels significantly inhibited adverse blood-material interactions such as hemolysis, complement activation, platelet activation and contact activation, and exhibited better anticoagulant properties than raw chitosan hydrogels.
32532397	3	30	theme	contact	700:706	arg1	activation					708:717	contact activation	700:717	contact activation	700:717	The C-K hydrogels significantly inhibited adverse blood-material interactions such as hemolysis, complement activation, platelet activation and contact activation, and exhibited better anticoagulant properties than raw chitosan hydrogels.
32532397	2	31	theme	X-ray	489:493	arg1	spectroscopy					509:520	X-ray photoelectron spectroscopy	489:520	X-ray photoelectron spectroscopy	489:520	The chemical compositions and morphologies of the developed C-K hydrogels were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and scanning electron microscopy.
32532397	1	32	theme	septic	324:329	arg1	blood					331:335	septic blood	324:335	septic blood	324:335	Here, we describe a green approach to fabricate genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood synchronously.
32532397	6	33	theme	C-K	1199:1201	arg1	sorbents					1241:1248	promising hemoperfusion sorbents	1217:1248	promising hemoperfusion sorbents	1217:1248	It is believed that the C-K hydrogels are promising hemoperfusion sorbents to treat severe septic patients.
32532397	6	33	theme	C-K	1199:1201	arg1	hydrogels					1203:1211	the C-K hydrogels	1195:1211	the C-K hydrogels	1195:1211	It is believed that the C-K hydrogels are promising hemoperfusion sorbents to treat severe septic patients.
32532397	2	34	theme	hydrogels	416:424	arg1	morphologies					382:393	morphologies	382:393	morphologies	382:393	The chemical compositions and morphologies of the developed C-K hydrogels were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and scanning electron microscopy.
32532397	2	34	theme	hydrogels	416:424	arg1	compositions					365:376	The chemical compositions	352:376	The chemical compositions	352:376	The chemical compositions and morphologies of the developed C-K hydrogels were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and scanning electron microscopy.
32532397	3	35	theme	raw	771:773	arg1	hydrogels					784:792	raw chitosan hydrogels	771:792	raw chitosan hydrogels	771:792	The C-K hydrogels significantly inhibited adverse blood-material interactions such as hemolysis, complement activation, platelet activation and contact activation, and exhibited better anticoagulant properties than raw chitosan hydrogels.
32532397	0	36	dep	endotoxin	85:93	arg1	cleansing					108:116	cleansing	108:116	cleansing	108:116	Anticoagulant chitosan-kappa-carrageenan composite hydrogel sorbent for simultaneous endotoxin and bacteria cleansing in septic blood.
32532397	1	37	theme	C-K	251:253	arg1	hydrogels					240:248	genipin crosslinked chitosan-kappa-carrageenan composite hydrogels	183:248	genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood	183:335	Here, we describe a green approach to fabricate genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood synchronously.
32532397	1	37	theme	C-K	251:253	arg1	hydrogels					255:263	C-K hydrogels	251:263	C-K hydrogels	251:263	Here, we describe a green approach to fabricate genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood synchronously.
32532397	4	38	theme	3-h	964:966	arg1	procedure					993:1001	a 3-h simulative hemoperfusion procedure	962:1001	a 3-h simulative hemoperfusion procedure	962:1001	Most importantly, the optimized C2-K1 hydrogels were competent to eliminate 63.3 % of endotoxin in septic blood with a maximum adsorption capacity of 95.0 EU/g during a 3-h simulative hemoperfusion procedure.
32532397	3	39	theme	anticoagulant	741:753	arg1	properties					755:764	better anticoagulant properties	734:764	better anticoagulant properties	734:764	The C-K hydrogels significantly inhibited adverse blood-material interactions such as hemolysis, complement activation, platelet activation and contact activation, and exhibited better anticoagulant properties than raw chitosan hydrogels.
32532397	0	40	theme	hydrogel	51:58	arg1	sorbent					60:66	composite hydrogel sorbent	41:66	composite hydrogel sorbent for simultaneous endotoxin and bacteria cleansing	41:116	Anticoagulant chitosan-kappa-carrageenan composite hydrogel sorbent for simultaneous endotoxin and bacteria cleansing in septic blood.
32532397	2	41	theme	electron	535:542	arg1	microscopy					544:553	scanning electron microscopy	526:553	scanning electron microscopy	526:553	The chemical compositions and morphologies of the developed C-K hydrogels were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and scanning electron microscopy.
32532397	5	42	theme	Bacteria	1004:1011	arg1	experiments					1023:1033	Bacteria cleansing experiments	1004:1033	Bacteria cleansing experiments	1004:1033	Bacteria cleansing experiments further demonstrated that the optimized C2-K1 hydrogels effectively decreased 46.0 % of E. coli and 68.7 % of S. aureus load, respectively.
32532397	5	43	theme	aureus	1148:1153	arg1	load					1155:1158	S. aureus load	1145:1158	S. aureus load	1145:1158	Bacteria cleansing experiments further demonstrated that the optimized C2-K1 hydrogels effectively decreased 46.0 % of E. coli and 68.7 % of S. aureus load, respectively.
32532397	6	44	theme	promising	1217:1225	arg1	sorbents					1241:1248	promising hemoperfusion sorbents	1217:1248	promising hemoperfusion sorbents	1217:1248	It is believed that the C-K hydrogels are promising hemoperfusion sorbents to treat severe septic patients.
32532397	6	44	theme	promising	1217:1225	arg1	hydrogels					1203:1211	the C-K hydrogels	1195:1211	the C-K hydrogels	1195:1211	It is believed that the C-K hydrogels are promising hemoperfusion sorbents to treat severe septic patients.
32532397	0	45	theme	simultaneous	72:83	arg1	endotoxin					85:93	simultaneous endotoxin	72:93	simultaneous endotoxin	72:93	Anticoagulant chitosan-kappa-carrageenan composite hydrogel sorbent for simultaneous endotoxin and bacteria cleansing in septic blood.
32532397	5	46	theme	load	1155:1158	arg1	%					1118:1118	46.0 %	1113:1118	46.0 % of E. coli	1113:1129	Bacteria cleansing experiments further demonstrated that the optimized C2-K1 hydrogels effectively decreased 46.0 % of E. coli and 68.7 % of S. aureus load, respectively.
32532397	5	46	theme	load	1155:1158	arg1	coli					1126:1129	E. coli	1123:1129	E. coli	1123:1129	Bacteria cleansing experiments further demonstrated that the optimized C2-K1 hydrogels effectively decreased 46.0 % of E. coli and 68.7 % of S. aureus load, respectively.
32532397	5	46	theme	load	1155:1158	arg1	%					1140:1140	68.7 %	1135:1140	68.7 % of S. aureus load	1135:1158	Bacteria cleansing experiments further demonstrated that the optimized C2-K1 hydrogels effectively decreased 46.0 % of E. coli and 68.7 % of S. aureus load, respectively.
32532397	5	46	theme	load	1155:1158	arg1	load					1155:1158	S. aureus load	1145:1158	S. aureus load	1145:1158	Bacteria cleansing experiments further demonstrated that the optimized C2-K1 hydrogels effectively decreased 46.0 % of E. coli and 68.7 % of S. aureus load, respectively.
32532397	4	47	theme	C2-K1	827:831	arg1	hydrogels					833:841	the optimized C2-K1 hydrogels	813:841	the optimized C2-K1 hydrogels	813:841	Most importantly, the optimized C2-K1 hydrogels were competent to eliminate 63.3 % of endotoxin in septic blood with a maximum adsorption capacity of 95.0 EU/g during a 3-h simulative hemoperfusion procedure.
32532397	4	47	theme	C2-K1	827:831	arg1	competent					848:856	competent	848:856	competent	848:856	Most importantly, the optimized C2-K1 hydrogels were competent to eliminate 63.3 % of endotoxin in septic blood with a maximum adsorption capacity of 95.0 EU/g during a 3-h simulative hemoperfusion procedure.
32532397	4	48	theme	adsorption	922:931	arg1	capacity					933:940	a maximum adsorption capacity	912:940	a maximum adsorption capacity of 95.0 EU/g during a 3-h simulative hemoperfusion procedure	912:1001	Most importantly, the optimized C2-K1 hydrogels were competent to eliminate 63.3 % of endotoxin in septic blood with a maximum adsorption capacity of 95.0 EU/g during a 3-h simulative hemoperfusion procedure.
32532397	5	49	theme	coli	1126:1129	arg1	%					1118:1118	46.0 %	1113:1118	46.0 % of E. coli	1113:1129	Bacteria cleansing experiments further demonstrated that the optimized C2-K1 hydrogels effectively decreased 46.0 % of E. coli and 68.7 % of S. aureus load, respectively.
32532397	5	49	theme	coli	1126:1129	arg1	coli					1126:1129	E. coli	1123:1129	E. coli	1123:1129	Bacteria cleansing experiments further demonstrated that the optimized C2-K1 hydrogels effectively decreased 46.0 % of E. coli and 68.7 % of S. aureus load, respectively.
32532397	5	49	theme	coli	1126:1129	arg1	%					1140:1140	68.7 %	1135:1140	68.7 % of S. aureus load	1135:1158	Bacteria cleansing experiments further demonstrated that the optimized C2-K1 hydrogels effectively decreased 46.0 % of E. coli and 68.7 % of S. aureus load, respectively.
32532397	5	49	theme	coli	1126:1129	arg1	load					1155:1158	S. aureus load	1145:1158	S. aureus load	1145:1158	Bacteria cleansing experiments further demonstrated that the optimized C2-K1 hydrogels effectively decreased 46.0 % of E. coli and 68.7 % of S. aureus load, respectively.
32532397	2	50	dep	transform	456:464	arg1	infrared					466:473	infrared	466:473	transform infrared spectroscopy, X-ray photoelectron spectroscopy and scanning electron microscopy	456:553	The chemical compositions and morphologies of the developed C-K hydrogels were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and scanning electron microscopy.
32532397	6	51	theme	hemoperfusion	1227:1239	arg1	sorbents					1241:1248	promising hemoperfusion sorbents	1217:1248	promising hemoperfusion sorbents	1217:1248	It is believed that the C-K hydrogels are promising hemoperfusion sorbents to treat severe septic patients.
32532397	6	51	theme	hemoperfusion	1227:1239	arg1	hydrogels					1203:1211	the C-K hydrogels	1195:1211	the C-K hydrogels	1195:1211	It is believed that the C-K hydrogels are promising hemoperfusion sorbents to treat severe septic patients.
32532397	1	52	dep	endotoxin	285:293	arg1	level					295:299	level	295:299	level	295:299	Here, we describe a green approach to fabricate genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood synchronously.
32532397	3	53	theme	better	734:739	arg1	properties					755:764	better anticoagulant properties	734:764	better anticoagulant properties	734:764	The C-K hydrogels significantly inhibited adverse blood-material interactions such as hemolysis, complement activation, platelet activation and contact activation, and exhibited better anticoagulant properties than raw chitosan hydrogels.
32532397	3	54	theme	C-K	560:562	arg1	hydrogels					564:572	The C-K hydrogels	556:572	The C-K hydrogels	556:572	The C-K hydrogels significantly inhibited adverse blood-material interactions such as hemolysis, complement activation, platelet activation and contact activation, and exhibited better anticoagulant properties than raw chitosan hydrogels.
32532397	5	55	theme	cleansing	1013:1021	arg1	experiments					1023:1033	Bacteria cleansing experiments	1004:1033	Bacteria cleansing experiments	1004:1033	Bacteria cleansing experiments further demonstrated that the optimized C2-K1 hydrogels effectively decreased 46.0 % of E. coli and 68.7 % of S. aureus load, respectively.
32532397	2	56	dep	Fourier	448:454	arg1	transform					456:464	transform	456:464	transform infrared spectroscopy, X-ray photoelectron spectroscopy and scanning electron microscopy	456:553	The chemical compositions and morphologies of the developed C-K hydrogels were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and scanning electron microscopy.
32532397	1	57	theme	green	155:159	arg1	approach					161:168	a green approach	153:168	a green approach to fabricate genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood synchronously	153:349	Here, we describe a green approach to fabricate genipin crosslinked chitosan-kappa-carrageenan composite hydrogels (C-K hydrogels) aiming at reducing endotoxin level and bacteria burden in septic blood synchronously.
32532397	0	58	theme	composite	41:49	arg1	sorbent					60:66	composite hydrogel sorbent	41:66	composite hydrogel sorbent for simultaneous endotoxin and bacteria cleansing	41:116	Anticoagulant chitosan-kappa-carrageenan composite hydrogel sorbent for simultaneous endotoxin and bacteria cleansing in septic blood.
32532397	2	59	theme	C-K	412:414	arg1	hydrogels					416:424	the developed C-K hydrogels	398:424	the developed C-K hydrogels	398:424	The chemical compositions and morphologies of the developed C-K hydrogels were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and scanning electron microscopy.
31903666	0	0	theme	mass	97:100	arg1	spectrometry					102:113	Orbitrap-technology-based mass spectrometry	71:113	Orbitrap-technology-based mass spectrometry	71:113	Development of two-dimensional gas chromatography (GC×GC) coupled with Orbitrap-technology-based mass spectrometry: Interest in the identification of biofuel composition.
31903666	3	1	theme	peaks	628:632	arg1	separation					601:610	the separation	597:610	the separation of chromatogram peaks	597:632	The results obtained in this study showed a significant improvement by using GC×GC-EI-MS in comparison with GC-EI-MS; the separation of chromatogram peaks was highly improved, which facilitated detection and identification.
31903666	1	2	theme	Comprehensive	171:183	arg1	GC					205:206	GC	205:206	GC	205:206	Comprehensive gas chromatography (GC) has emerged in recent years as the technique of choice for the analysis of volatile and semivolatile compounds in complex matrices.
31903666	1	2	theme	Comprehensive	171:183	arg1	chromatography					189:202	Comprehensive gas chromatography	171:202	Comprehensive gas chromatography (GC)	171:207	Comprehensive gas chromatography (GC) has emerged in recent years as the technique of choice for the analysis of volatile and semivolatile compounds in complex matrices.
31903666	0	3	theme	Orbitrap-technology-based	71:95	arg1	spectrometry					102:113	Orbitrap-technology-based mass spectrometry	71:113	Orbitrap-technology-based mass spectrometry	71:113	Development of two-dimensional gas chromatography (GC×GC) coupled with Orbitrap-technology-based mass spectrometry: Interest in the identification of biofuel composition.
31903666	4	4	from	power	879:883	arg1	Hz					856:857	about 25 Hz	847:857	about 25 Hz	847:857	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	4	4	from	power	879:883	arg1	frequency					825:833	the frequency average	821:841	the frequency average	821:841	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	4	5	theme	mass	864:867	arg1	power					879:883	a mass resolving power	862:883	a mass resolving power	862:883	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	4	5	theme	mass	864:867	arg1	sufficient					912:921	sufficient	912:921	sufficient	912:921	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	1	6	theme	gas	185:187	arg1	GC					205:206	GC	205:206	GC	205:206	Comprehensive gas chromatography (GC) has emerged in recent years as the technique of choice for the analysis of volatile and semivolatile compounds in complex matrices.
31903666	1	6	theme	gas	185:187	arg1	chromatography					189:202	Comprehensive gas chromatography	171:202	Comprehensive gas chromatography (GC)	171:207	Comprehensive gas chromatography (GC) has emerged in recent years as the technique of choice for the analysis of volatile and semivolatile compounds in complex matrices.
31903666	1	7	theme	choice	257:262	arg1	technique					244:252	the technique	240:252	the technique of choice for the analysis of volatile and semivolatile compounds in complex matrices	240:338	Comprehensive gas chromatography (GC) has emerged in recent years as the technique of choice for the analysis of volatile and semivolatile compounds in complex matrices.
31903666	4	8	theme	15.000	888:893	arg1	power					879:883	a mass resolving power	862:883	a mass resolving power	862:883	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	4	8	theme	15.000	888:893	arg1	sufficient					912:921	sufficient	912:921	sufficient	912:921	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	4	9	theme	time-of-flight	767:780	arg1	analyzer					782:789	time-of-flight analyzer	767:789	time-of-flight analyzer	767:789	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	7	10	theme	GC×GC-MS	1367:1374	arg1	peaks					1376:1380	the GC×GC-MS peaks	1363:1380	the GC×GC-MS peaks	1363:1380	At a mass resolving power of 7.500, the scan rate reaches 43 Hz and the GC×GC-MS peaks can be represented by more than 10 data points, which should be sufficient for quantification.
31903666	1	11	from	compounds	310:318	arg1	matrices					331:338	complex matrices	323:338	complex matrices	323:338	Comprehensive gas chromatography (GC) has emerged in recent years as the technique of choice for the analysis of volatile and semivolatile compounds in complex matrices.
31903666	9	12	theme	fragment	1657:1664	arg1	ions					1666:1669	molecular and fragment ions	1643:1669	ions	1666:1669	Following this, a highly resolved chromatogram was obtained, allowing EI mass spectra containing molecular and fragment ions of many distinct molecules present in the sample to be identified.
31903666	2	13	theme	high-resolution	358:372	arg1	MS					393:394	MS	393:394	MS	393:394	Coupling it with high-resolution mass spectrometry (MS) makes a powerful tool for identification and quantification of organic compounds.
31903666	2	13	theme	high-resolution	358:372	arg1	spectrometry					379:390	high-resolution mass spectrometry	358:390	high-resolution mass spectrometry (MS)	358:395	Coupling it with high-resolution mass spectrometry (MS) makes a powerful tool for identification and quantification of organic compounds.
31903666	4	14	theme	mass	739:742	arg1	analyzer					744:751	Orbitrap mass analyzer	730:751	Orbitrap mass analyzer compared with time-of-flight analyzer	730:789	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	4	15	theme	narrowest	960:968	arg1	peaks					986:990	the narrowest chromatographic peaks	956:990	the narrowest chromatographic peaks	956:990	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	6	16	theme	average	1117:1123	arg1	accuracy					1130:1137	this average mass accuracy	1112:1137	this average mass accuracy	1112:1137	Within this average mass accuracy, some reasonable elemental compositions can be proposed and combined with characteristic fragment ions, and the molecules can be identified with precision.
31903666	9	17	theme	EI	1616:1617	arg1	spectra					1624:1630	EI mass spectra	1616:1630	EI mass spectra containing molecular and fragment ions of many distinct molecules present in the sample to be identified	1616:1735	Following this, a highly resolved chromatogram was obtained, allowing EI mass spectra containing molecular and fragment ions of many distinct molecules present in the sample to be identified.
31903666	4	18	theme	acquisition	803:813	arg1	rate					815:818	the data acquisition rate	794:818	the data acquisition rate	794:818	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	4	18	theme	acquisition	803:813	arg1	limitation					716:725	the limitation	712:725	the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer	712:789	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	7	19	dep	rate	1340:1343	arg1	scan					1335:1338	scan	1335:1338	scan	1335:1338	At a mass resolving power of 7.500, the scan rate reaches 43 Hz and the GC×GC-MS peaks can be represented by more than 10 data points, which should be sufficient for quantification.
31903666	9	20	attach	present	1698:1704	arg2	molecules					1688:1696	many distinct molecules	1674:1696	many distinct molecules present in the sample to be identified	1674:1735	Following this, a highly resolved chromatogram was obtained, allowing EI mass spectra containing molecular and fragment ions of many distinct molecules present in the sample to be identified.
31903666	9	20	attach	present	1698:1704	arg1	sample					1713:1718	the sample to be identified	1709:1735	the sample to be identified	1709:1735	Following this, a highly resolved chromatogram was obtained, allowing EI mass spectra containing molecular and fragment ions of many distinct molecules present in the sample to be identified.
31903666	9	21	theme	mass	1619:1622	arg1	spectra					1624:1630	EI mass spectra	1616:1630	EI mass spectra containing molecular and fragment ions of many distinct molecules present in the sample to be identified	1616:1735	Following this, a highly resolved chromatogram was obtained, allowing EI mass spectra containing molecular and fragment ions of many distinct molecules present in the sample to be identified.
31903666	4	22	theme	data	798:801	arg1	rate					815:818	the data acquisition rate	794:818	the data acquisition rate	794:818	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	4	22	theme	data	798:801	arg1	limitation					716:725	the limitation	712:725	the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer	712:789	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	4	23	theme	proper	931:936	arg1	reconstruction					938:951	the proper reconstruction	927:951	the proper reconstruction of the narrowest chromatographic peaks	927:990	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	5	24	theme	ppm	1100:1102	arg1	accuracy					1080:1087	an average mass accuracy	1064:1087	an average mass accuracy of about 1 ppm	1064:1102	On the other hand, the different spectra obtained in this study showed an average mass accuracy of about 1 ppm.
31903666	1	25	from	analysis	272:279	arg1	matrices					331:338	complex matrices	323:338	complex matrices	323:338	Comprehensive gas chromatography (GC) has emerged in recent years as the technique of choice for the analysis of volatile and semivolatile compounds in complex matrices.
31903666	9	26	theme	molecules	1688:1696	arg1	ions					1666:1669	molecular and fragment ions	1643:1669	ions	1666:1669	Following this, a highly resolved chromatogram was obtained, allowing EI mass spectra containing molecular and fragment ions of many distinct molecules present in the sample to be identified.
31903666	6	27	theme	fragment	1228:1235	arg1	ions					1237:1240	characteristic fragment ions	1213:1240	characteristic fragment ions	1213:1240	Within this average mass accuracy, some reasonable elemental compositions can be proposed and combined with characteristic fragment ions, and the molecules can be identified with precision.
31903666	7	28	theme	data	1417:1420	arg1	points					1422:1427	more than 10 data points	1404:1427	more than 10 data points	1404:1427	At a mass resolving power of 7.500, the scan rate reaches 43 Hz and the GC×GC-MS peaks can be represented by more than 10 data points, which should be sufficient for quantification.
31903666	7	29	theme	mass	1300:1303	arg1	power					1315:1319	a mass resolving power	1298:1319	a mass resolving power of 7.500	1298:1328	At a mass resolving power of 7.500, the scan rate reaches 43 Hz and the GC×GC-MS peaks can be represented by more than 10 data points, which should be sufficient for quantification.
31903666	3	30	with	comparison	571:580	arg1	GC-EI-MS					587:594	GC-EI-MS	587:594	GC-EI-MS	587:594	The results obtained in this study showed a significant improvement by using GC×GC-EI-MS in comparison with GC-EI-MS; the separation of chromatogram peaks was highly improved, which facilitated detection and identification.
31903666	9	31	theme	present	1698:1704	arg1	molecules					1688:1696	many distinct molecules	1674:1696	many distinct molecules present in the sample to be identified	1674:1735	Following this, a highly resolved chromatogram was obtained, allowing EI mass spectra containing molecular and fragment ions of many distinct molecules present in the sample to be identified.
31903666	4	32	theme	resolving	869:877	arg1	power					879:883	a mass resolving power	862:883	a mass resolving power	862:883	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	4	32	theme	resolving	869:877	arg1	sufficient					912:921	sufficient	912:921	sufficient	912:921	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	0	33	theme	gas	31:33	arg1	GC×GC					51:55	GC×GC	51:55	GC×GC	51:55	Development of two-dimensional gas chromatography (GC×GC) coupled with Orbitrap-technology-based mass spectrometry: Interest in the identification of biofuel composition.
31903666	0	33	theme	gas	31:33	arg1	chromatography					35:48	two-dimensional gas chromatography	15:48	two-dimensional gas chromatography (GC×GC) coupled with Orbitrap-technology-based mass spectrometry	15:113	Development of two-dimensional gas chromatography (GC×GC) coupled with Orbitrap-technology-based mass spectrometry: Interest in the identification of biofuel composition.
31903666	7	34	theme	7.500	1324:1328	arg1	power					1315:1319	a mass resolving power	1298:1319	a mass resolving power of 7.500	1298:1328	At a mass resolving power of 7.500, the scan rate reaches 43 Hz and the GC×GC-MS peaks can be represented by more than 10 data points, which should be sufficient for quantification.
31903666	9	35	theme	molecular	1643:1651	arg1	ions					1666:1669	molecular and fragment ions	1643:1669	ions	1666:1669	Following this, a highly resolved chromatogram was obtained, allowing EI mass spectra containing molecular and fragment ions of many distinct molecules present in the sample to be identified.
31903666	9	36	contain	containing	1632:1641	arg2	ions					1666:1669	molecular and fragment ions	1643:1669	ions	1666:1669	Following this, a highly resolved chromatogram was obtained, allowing EI mass spectra containing molecular and fragment ions of many distinct molecules present in the sample to be identified.
31903666	9	36	contain	containing	1632:1641	arg1	spectra					1624:1630	EI mass spectra	1616:1630	EI mass spectra containing molecular and fragment ions of many distinct molecules present in the sample to be identified	1616:1735	Following this, a highly resolved chromatogram was obtained, allowing EI mass spectra containing molecular and fragment ions of many distinct molecules present in the sample to be identified.
31903666	0	37	theme	two-dimensional	15:29	arg1	GC×GC					51:55	GC×GC	51:55	GC×GC	51:55	Development of two-dimensional gas chromatography (GC×GC) coupled with Orbitrap-technology-based mass spectrometry: Interest in the identification of biofuel composition.
31903666	0	37	theme	two-dimensional	15:29	arg1	chromatography					35:48	two-dimensional gas chromatography	15:48	two-dimensional gas chromatography (GC×GC) coupled with Orbitrap-technology-based mass spectrometry	15:113	Development of two-dimensional gas chromatography (GC×GC) coupled with Orbitrap-technology-based mass spectrometry: Interest in the identification of biofuel composition.
31903666	1	38	theme	volatile	284:291	arg1	compounds					310:318	volatile and semivolatile compounds	284:318	volatile and semivolatile compounds in complex matrices	284:338	Comprehensive gas chromatography (GC) has emerged in recent years as the technique of choice for the analysis of volatile and semivolatile compounds in complex matrices.
31903666	6	39	theme	elemental	1156:1164	arg1	compositions					1166:1177	some reasonable elemental compositions	1140:1177	some reasonable elemental compositions	1140:1177	Within this average mass accuracy, some reasonable elemental compositions can be proposed and combined with characteristic fragment ions, and the molecules can be identified with precision.
31903666	2	40	dep	Coupling	341:348	arg1	it					350:351	it	350:351	it	350:351	Coupling it with high-resolution mass spectrometry (MS) makes a powerful tool for identification and quantification of organic compounds.
31903666	8	41	theme	bio-oil	1530:1536	arg1	sample					1538:1543	a cellulose bio-oil sample	1518:1543	a cellulose bio-oil sample	1518:1543	The GC×GC-MS was also applied to analyze a cellulose bio-oil sample.
31903666	0	42	theme	biofuel	150:156	arg1	composition					158:168	biofuel composition	150:168	biofuel composition	150:168	Development of two-dimensional gas chromatography (GC×GC) coupled with Orbitrap-technology-based mass spectrometry: Interest in the identification of biofuel composition.
31903666	5	43	theme	average	1067:1073	arg1	accuracy					1080:1087	an average mass accuracy	1064:1087	an average mass accuracy of about 1 ppm	1064:1102	On the other hand, the different spectra obtained in this study showed an average mass accuracy of about 1 ppm.
31903666	7	44	theme	resolving	1305:1313	arg1	power					1315:1319	a mass resolving power	1298:1319	a mass resolving power of 7.500	1298:1328	At a mass resolving power of 7.500, the scan rate reaches 43 Hz and the GC×GC-MS peaks can be represented by more than 10 data points, which should be sufficient for quantification.
31903666	2	45	theme	powerful	405:412	arg1	tool					414:417	a powerful tool	403:417	a powerful tool for identification and quantification of organic compounds	403:476	Coupling it with high-resolution mass spectrometry (MS) makes a powerful tool for identification and quantification of organic compounds.
31903666	0	46	theme	chromatography	35:48	arg1	Development					0:10	Development	0:10	Development of two-dimensional gas chromatography (GC×GC) coupled with Orbitrap-technology-based mass spectrometry: Interest in the identification of biofuel composition.	0:169	Development of two-dimensional gas chromatography (GC×GC) coupled with Orbitrap-technology-based mass spectrometry: Interest in the identification of biofuel composition.
31903666	1	47	theme	semivolatile	297:308	arg1	compounds					310:318	volatile and semivolatile compounds	284:318	volatile and semivolatile compounds in complex matrices	284:338	Comprehensive gas chromatography (GC) has emerged in recent years as the technique of choice for the analysis of volatile and semivolatile compounds in complex matrices.
31903666	5	48	theme	mass	1075:1078	arg1	accuracy					1080:1087	an average mass accuracy	1064:1087	an average mass accuracy of about 1 ppm	1064:1102	On the other hand, the different spectra obtained in this study showed an average mass accuracy of about 1 ppm.
31903666	4	49	theme	average	835:841	arg1	Hz					856:857	about 25 Hz	847:857	about 25 Hz	847:857	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	4	49	theme	average	835:841	arg1	frequency					825:833	the frequency average	821:841	the frequency average	821:841	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	9	50	theme	resolved	1571:1578	arg1	chromatogram					1580:1591	a highly resolved chromatogram	1562:1591	a highly resolved chromatogram	1562:1591	Following this, a highly resolved chromatogram was obtained, allowing EI mass spectra containing molecular and fragment ions of many distinct molecules present in the sample to be identified.
31903666	1	51	theme	recent	224:229	arg1	years					231:235	recent years	224:235	recent years	224:235	Comprehensive gas chromatography (GC) has emerged in recent years as the technique of choice for the analysis of volatile and semivolatile compounds in complex matrices.
31903666	2	52	theme	compounds	468:476	arg1	quantification					442:455	quantification	442:455	quantification	442:455	Coupling it with high-resolution mass spectrometry (MS) makes a powerful tool for identification and quantification of organic compounds.
31903666	2	52	theme	compounds	468:476	arg1	identification					423:436	identification	423:436	identification	423:436	Coupling it with high-resolution mass spectrometry (MS) makes a powerful tool for identification and quantification of organic compounds.
31903666	1	53	theme	compounds	310:318	arg1	analysis					272:279	the analysis	268:279	the analysis of volatile and semivolatile compounds in complex matrices	268:338	Comprehensive gas chromatography (GC) has emerged in recent years as the technique of choice for the analysis of volatile and semivolatile compounds in complex matrices.
31903666	2	54	with	Coupling	341:348	arg1	MS					393:394	MS	393:394	MS	393:394	Coupling it with high-resolution mass spectrometry (MS) makes a powerful tool for identification and quantification of organic compounds.
31903666	2	54	with	Coupling	341:348	arg1	spectrometry					379:390	high-resolution mass spectrometry	358:390	high-resolution mass spectrometry (MS)	358:395	Coupling it with high-resolution mass spectrometry (MS) makes a powerful tool for identification and quantification of organic compounds.
31903666	6	55	theme	characteristic	1213:1226	arg1	ions					1237:1240	characteristic fragment ions	1213:1240	characteristic fragment ions	1213:1240	Within this average mass accuracy, some reasonable elemental compositions can be proposed and combined with characteristic fragment ions, and the molecules can be identified with precision.
31903666	4	56	theme	Orbitrap	730:737	arg1	analyzer					744:751	Orbitrap mass analyzer	730:751	Orbitrap mass analyzer compared with time-of-flight analyzer	730:789	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	2	57	theme	organic	460:466	arg1	compounds					468:476	organic compounds	460:476	organic compounds	460:476	Coupling it with high-resolution mass spectrometry (MS) makes a powerful tool for identification and quantification of organic compounds.
31903666	0	58	theme	composition	158:168	arg1	identification					132:145	the identification	128:145	the identification of biofuel composition	128:168	Development of two-dimensional gas chromatography (GC×GC) coupled with Orbitrap-technology-based mass spectrometry: Interest in the identification of biofuel composition.
31903666	9	59	from	sample	1713:1718	arg1	present					1698:1704	present	1698:1704	present	1698:1704	Following this, a highly resolved chromatogram was obtained, allowing EI mass spectra containing molecular and fragment ions of many distinct molecules present in the sample to be identified.
31903666	4	60	theme	chromatographic	970:984	arg1	peaks					986:990	the narrowest chromatographic peaks	956:990	the narrowest chromatographic peaks	956:990	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	1	61	theme	complex	323:329	arg1	matrices					331:338	complex matrices	323:338	complex matrices	323:338	Comprehensive gas chromatography (GC) has emerged in recent years as the technique of choice for the analysis of volatile and semivolatile compounds in complex matrices.
31903666	9	62	theme	distinct	1679:1686	arg1	molecules					1688:1696	many distinct molecules	1674:1696	many distinct molecules present in the sample to be identified	1674:1735	Following this, a highly resolved chromatogram was obtained, allowing EI mass spectra containing molecular and fragment ions of many distinct molecules present in the sample to be identified.
31903666	0	63	from	Interest	116:123	arg1	identification					132:145	the identification	128:145	the identification of biofuel composition	128:168	Development of two-dimensional gas chromatography (GC×GC) coupled with Orbitrap-technology-based mass spectrometry: Interest in the identification of biofuel composition.
31903666	9	64	theme	many	1674:1677	arg1	molecules					1688:1696	many distinct molecules	1674:1696	many distinct molecules present in the sample to be identified	1674:1735	Following this, a highly resolved chromatogram was obtained, allowing EI mass spectra containing molecular and fragment ions of many distinct molecules present in the sample to be identified.
31903666	5	65	theme	different	1016:1024	arg1	spectra					1026:1032	the different spectra	1012:1032	the different spectra obtained in this study	1012:1055	On the other hand, the different spectra obtained in this study showed an average mass accuracy of about 1 ppm.
31903666	4	66	theme	analyzer	744:751	arg1	rate					815:818	the data acquisition rate	794:818	the data acquisition rate	794:818	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	4	66	theme	analyzer	744:751	arg1	limitation					716:725	the limitation	712:725	the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer	712:789	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	6	67	theme	reasonable	1145:1154	arg1	compositions					1166:1177	some reasonable elemental compositions	1140:1177	some reasonable elemental compositions	1140:1177	Within this average mass accuracy, some reasonable elemental compositions can be proposed and combined with characteristic fragment ions, and the molecules can be identified with precision.
31903666	9	68	from	present	1698:1704	arg1	sample					1713:1718	the sample to be identified	1709:1735	the sample to be identified	1709:1735	Following this, a highly resolved chromatogram was obtained, allowing EI mass spectra containing molecular and fragment ions of many distinct molecules present in the sample to be identified.
31903666	5	69	theme	other	1000:1004	arg1	hand					1006:1009	the other hand	996:1009	the other hand	996:1009	On the other hand, the different spectra obtained in this study showed an average mass accuracy of about 1 ppm.
31903666	3	70	theme	significant	523:533	arg1	improvement					535:545	a significant improvement	521:545	a significant improvement	521:545	The results obtained in this study showed a significant improvement by using GC×GC-EI-MS in comparison with GC-EI-MS; the separation of chromatogram peaks was highly improved, which facilitated detection and identification.
31903666	4	71	theme	peaks	986:990	arg1	reconstruction					938:951	the proper reconstruction	927:951	the proper reconstruction of the narrowest chromatographic peaks	927:990	However, the limitation of Orbitrap mass analyzer compared with time-of-flight analyzer is the data acquisition rate; the frequency average was about 25 Hz at a mass resolving power of 15.000, which is barely sufficient for the proper reconstruction of the narrowest chromatographic peaks.
31903666	3	72	theme	chromatogram	615:626	arg1	peaks					628:632	chromatogram peaks	615:632	chromatogram peaks	615:632	The results obtained in this study showed a significant improvement by using GC×GC-EI-MS in comparison with GC-EI-MS; the separation of chromatogram peaks was highly improved, which facilitated detection and identification.
31903666	8	73	theme	cellulose	1520:1528	arg1	sample					1538:1543	a cellulose bio-oil sample	1518:1543	a cellulose bio-oil sample	1518:1543	The GC×GC-MS was also applied to analyze a cellulose bio-oil sample.
31903666	1	74	from	matrices	331:338	arg1	analysis					272:279	the analysis	268:279	the analysis of volatile and semivolatile compounds in complex matrices	268:338	Comprehensive gas chromatography (GC) has emerged in recent years as the technique of choice for the analysis of volatile and semivolatile compounds in complex matrices.
31903666	6	75	theme	mass	1125:1128	arg1	accuracy					1130:1137	this average mass accuracy	1112:1137	this average mass accuracy	1112:1137	Within this average mass accuracy, some reasonable elemental compositions can be proposed and combined with characteristic fragment ions, and the molecules can be identified with precision.
31903666	0	76	dep	Development	0:10	arg1	Interest					116:123	Interest	116:123	Development of two-dimensional gas chromatography (GC×GC) coupled with Orbitrap-technology-based mass spectrometry: Interest in the identification of biofuel composition.	0:169	Development of two-dimensional gas chromatography (GC×GC) coupled with Orbitrap-technology-based mass spectrometry: Interest in the identification of biofuel composition.
31903666	2	77	theme	mass	374:377	arg1	MS					393:394	MS	393:394	MS	393:394	Coupling it with high-resolution mass spectrometry (MS) makes a powerful tool for identification and quantification of organic compounds.
31903666	2	77	theme	mass	374:377	arg1	spectrometry					379:390	high-resolution mass spectrometry	358:390	high-resolution mass spectrometry (MS)	358:395	Coupling it with high-resolution mass spectrometry (MS) makes a powerful tool for identification and quantification of organic compounds.
32846598	13	0	theme	components	2057:2066	arg1	inflation					2035:2043	the artificial inflation	2020:2043	the artificial inflation of valuable components, the resulting potential profit, and the gaps in detection	2020:2125	Combining the artificial inflation of valuable components, the resulting potential profit, and the gaps in detection, it appears that the whey protein isolates deserve particular attention when thinking like a criminal.
32846598	2	1	theme	common	343:348	arg1	adulterants					355:365	common milk adulterants	343:365	common milk adulterants	343:365	The current study explores the impact of common milk adulterants on the apparent compositional parameters of milk from the Dutch market as measured by standardized Fourier transform infrared (FTIR) spectroscopy.
32846598	7	2	theme	nitrogen-based	1155:1168	arg1	adulterants					1170:1180	five nitrogen-based adulterants	1150:1180	five nitrogen-based adulterants	1150:1180	These substances comprised five protein-rich adulterants, five nitrogen-based adulterants, seven carbohydrate-based adulterants, six preservatives and water, resulting in a set of 360 samples to be analysed.
32846598	10	3	theme	preservatives	1796:1808	arg1	Addition					1784:1791	Addition	1784:1791	Addition of preservatives	1784:1808	Addition of preservatives also went unnoticed.
32846598	1	4	theme	high-risk	265:273	arg1	components					223:232	its valuable components	210:232	its valuable components	210:232	Milk is regarded as one of the top food products susceptible to adulteration where its valuable components are specifically identified as high-risk indicators for milk fraud.
32846598	1	4	theme	high-risk	265:273	arg1	indicators					275:284	high-risk indicators	265:284	high-risk indicators for milk fraud	265:299	Milk is regarded as one of the top food products susceptible to adulteration where its valuable components are specifically identified as high-risk indicators for milk fraud.
32846598	9	5	theme	apparent	1729:1736	arg1	concentrations					1738:1751	unusual apparent concentrations	1721:1751	unusual apparent concentrations at lower concentration levels	1721:1781	When considering the compositional parameters univariately, especially protein- and nitrogen-based adulterants did not raise a flag of unusual apparent concentrations at lower concentration levels.
32846598	2	6	from	impact	333:338	arg1	parameters					397:406	the apparent compositional parameters	370:406	the apparent compositional parameters of milk from the Dutch market	370:436	The current study explores the impact of common milk adulterants on the apparent compositional parameters of milk from the Dutch market as measured by standardized Fourier transform infrared (FTIR) spectroscopy.
32846598	13	7	theme	artificial	2024:2033	arg1	inflation					2035:2043	the artificial inflation	2020:2043	the artificial inflation of valuable components, the resulting potential profit, and the gaps in detection	2020:2125	Combining the artificial inflation of valuable components, the resulting potential profit, and the gaps in detection, it appears that the whey protein isolates deserve particular attention when thinking like a criminal.
32846598	5	8	theme	economic	885:892	arg1	impact					894:899	The potential economic impact	871:899	The potential economic impact of these adulterations under a milk payment scheme	871:950	The potential economic impact of these adulterations under a milk payment scheme is also evaluated.
32846598	7	9	theme	protein-rich	1124:1135	arg1	adulterants					1137:1147	five protein-rich adulterants	1119:1147	five protein-rich adulterants	1119:1147	These substances comprised five protein-rich adulterants, five nitrogen-based adulterants, seven carbohydrate-based adulterants, six preservatives and water, resulting in a set of 360 samples to be analysed.
32846598	3	10	theme	adulterants	569:579	arg1	detectability					546:558	the detectability	542:558	the detectability of these adulterants at various concentration levels using the compositional parameters individually, in a univariate manner, and together in a multivariate approach	542:724	More precisely, it examines the detectability of these adulterants at various concentration levels using the compositional parameters individually, in a univariate manner, and together in a multivariate approach.
32846598	1	11	theme	products	167:174	arg1	Milk					127:130	Milk	127:130	Milk	127:130	Milk is regarded as one of the top food products susceptible to adulteration where its valuable components are specifically identified as high-risk indicators for milk fraud.
32846598	1	11	theme	products	167:174	arg1	products					167:174	the top food products	154:174	the top food products susceptible to adulteration where its valuable components are specifically identified as high-risk indicators for milk fraud	154:299	Milk is regarded as one of the top food products susceptible to adulteration where its valuable components are specifically identified as high-risk indicators for milk fraud.
32846598	1	11	theme	products	167:174	arg1	one					147:149	one	147:149	one	147:149	Milk is regarded as one of the top food products susceptible to adulteration where its valuable components are specifically identified as high-risk indicators for milk fraud.
32846598	9	12	theme	concentration	1762:1774	arg1	levels					1776:1781	lower concentration levels	1756:1781	lower concentration levels	1756:1781	When considering the compositional parameters univariately, especially protein- and nitrogen-based adulterants did not raise a flag of unusual apparent concentrations at lower concentration levels.
32846598	13	13	from	detection	2117:2125	arg1	inflation					2035:2043	the artificial inflation	2020:2043	the artificial inflation of valuable components, the resulting potential profit, and the gaps in detection	2020:2125	Combining the artificial inflation of valuable components, the resulting potential profit, and the gaps in detection, it appears that the whey protein isolates deserve particular attention when thinking like a criminal.
32846598	8	14	theme	adulterants	1486:1496	arg1	addition					1455:1462	the addition	1451:1462	the addition of carbohydrate-based adulterants, whey protein isolate, and skimmed milk powder,	1451:1544	The results showed that the addition of protein-rich adulterants, as well as dicyandiamide and melamine, increased the apparent protein content, while the addition of carbohydrate-based adulterants, whey protein isolate, and skimmed milk powder, increased the apparent lactose content.
32846598	13	15	from	gaps	2109:2112	arg1	detection					2117:2125	detection	2117:2125	detection	2117:2125	Combining the artificial inflation of valuable components, the resulting potential profit, and the gaps in detection, it appears that the whey protein isolates deserve particular attention when thinking like a criminal.
32846598	8	16	theme	whey	1499:1502	arg1	isolate					1512:1518	whey protein isolate	1499:1518	whey protein isolate	1499:1518	The results showed that the addition of protein-rich adulterants, as well as dicyandiamide and melamine, increased the apparent protein content, while the addition of carbohydrate-based adulterants, whey protein isolate, and skimmed milk powder, increased the apparent lactose content.
32846598	3	17	theme	various	584:590	arg1	levels					606:611	various concentration levels	584:611	various concentration levels using the compositional parameters individually, in a univariate manner, and together in a multivariate approach	584:724	More precisely, it examines the detectability of these adulterants at various concentration levels using the compositional parameters individually, in a univariate manner, and together in a multivariate approach.
32846598	5	18	theme	payment	937:943	arg1	scheme					945:950	a milk payment scheme	930:950	a milk payment scheme	930:950	The potential economic impact of these adulterations under a milk payment scheme is also evaluated.
32846598	9	19	from	flag	1713:1716	arg1	levels					1776:1781	lower concentration levels	1756:1781	lower concentration levels	1756:1781	When considering the compositional parameters univariately, especially protein- and nitrogen-based adulterants did not raise a flag of unusual apparent concentrations at lower concentration levels.
32846598	8	20	theme	isolate	1512:1518	arg1	addition					1455:1462	the addition	1451:1462	the addition of carbohydrate-based adulterants, whey protein isolate, and skimmed milk powder,	1451:1544	The results showed that the addition of protein-rich adulterants, as well as dicyandiamide and melamine, increased the apparent protein content, while the addition of carbohydrate-based adulterants, whey protein isolate, and skimmed milk powder, increased the apparent lactose content.
32846598	8	21	theme	protein-rich	1340:1351	arg1	adulterants					1353:1363	protein-rich adulterants	1340:1363	protein-rich adulterants	1340:1363	The results showed that the addition of protein-rich adulterants, as well as dicyandiamide and melamine, increased the apparent protein content, while the addition of carbohydrate-based adulterants, whey protein isolate, and skimmed milk powder, increased the apparent lactose content.
32846598	8	22	theme	apparent	1419:1426	arg1	content					1436:1442	the apparent protein content	1415:1442	the apparent protein content	1415:1442	The results showed that the addition of protein-rich adulterants, as well as dicyandiamide and melamine, increased the apparent protein content, while the addition of carbohydrate-based adulterants, whey protein isolate, and skimmed milk powder, increased the apparent lactose content.
32846598	13	23	from	inflation	2035:2043	arg1	detection					2117:2125	detection	2117:2125	detection	2117:2125	Combining the artificial inflation of valuable components, the resulting potential profit, and the gaps in detection, it appears that the whey protein isolates deserve particular attention when thinking like a criminal.
32846598	7	24	theme	samples	1276:1282	arg1	set					1265:1267	a set	1263:1267	a set of 360 samples to be analysed	1263:1297	These substances comprised five protein-rich adulterants, five nitrogen-based adulterants, seven carbohydrate-based adulterants, six preservatives and water, resulting in a set of 360 samples to be analysed.
32846598	7	24	theme	samples	1276:1282	arg1	samples					1276:1282	360 samples	1272:1282	360 samples	1272:1282	These substances comprised five protein-rich adulterants, five nitrogen-based adulterants, seven carbohydrate-based adulterants, six preservatives and water, resulting in a set of 360 samples to be analysed.
32846598	5	25	theme	adulterations	910:922	arg1	impact					894:899	The potential economic impact	871:899	The potential economic impact of these adulterations under a milk payment scheme	871:950	The potential economic impact of these adulterations under a milk payment scheme is also evaluated.
32846598	6	26	theme	milk	1037:1040	arg1	adulterations					1042:1054	milk adulterations	1037:1054	milk adulterations	1037:1054	Twenty-four substances were used to produce various categories of milk adulterations, each at four concentration levels.
32846598	8	27	theme	milk	1533:1536	arg1	powder					1538:1543	skimmed milk powder	1525:1543	skimmed milk powder	1525:1543	The results showed that the addition of protein-rich adulterants, as well as dicyandiamide and melamine, increased the apparent protein content, while the addition of carbohydrate-based adulterants, whey protein isolate, and skimmed milk powder, increased the apparent lactose content.
32846598	13	28	theme	protein	2153:2159	arg1	isolates					2161:2168	the whey protein isolates	2144:2168	the whey protein isolates	2144:2168	Combining the artificial inflation of valuable components, the resulting potential profit, and the gaps in detection, it appears that the whey protein isolates deserve particular attention when thinking like a criminal.
32846598	8	29	theme	dicyandiamide	1377:1389	arg1	addition					1328:1335	the addition	1324:1335	the addition of protein-rich adulterants, as well as dicyandiamide and melamine,	1324:1403	The results showed that the addition of protein-rich adulterants, as well as dicyandiamide and melamine, increased the apparent protein content, while the addition of carbohydrate-based adulterants, whey protein isolate, and skimmed milk powder, increased the apparent lactose content.
32846598	0	30	theme	common	87:92	arg1	measurements					113:124	common, standardized FTIR measurements	87:124	common, standardized FTIR measurements	87:124	Opportunities for fraudsters: When would profitable milk adulterations go unnoticed by common, standardized FTIR measurements?
32846598	2	31	dep	Fourier	466:472	arg1	transform					474:482	transform	474:482	transform infrared (FTIR) spectroscopy	474:511	The current study explores the impact of common milk adulterants on the apparent compositional parameters of milk from the Dutch market as measured by standardized Fourier transform infrared (FTIR) spectroscopy.
32846598	9	32	from	levels	1776:1781	arg1	concentrations					1738:1751	unusual apparent concentrations	1721:1751	unusual apparent concentrations at lower concentration levels	1721:1781	When considering the compositional parameters univariately, especially protein- and nitrogen-based adulterants did not raise a flag of unusual apparent concentrations at lower concentration levels.
32846598	9	32	from	levels	1776:1781	arg1	flag					1713:1716	a flag	1711:1716	a flag of unusual apparent concentrations at lower concentration levels	1711:1781	When considering the compositional parameters univariately, especially protein- and nitrogen-based adulterants did not raise a flag of unusual apparent concentrations at lower concentration levels.
32846598	12	33	theme	corn	1967:1970	arg1	starch					1972:1977	corn starch	1967:1977	corn starch	1967:1977	Regarding the potential profit of milk adulteration, whey protein and corn starch seem particularly interesting.
32846598	0	34	dep	common	87:92	arg1	standardized					95:106	standardized	95:106	standardized	95:106	Opportunities for fraudsters: When would profitable milk adulterations go unnoticed by common, standardized FTIR measurements?
32846598	8	35	theme	lactose	1569:1575	arg1	content					1577:1583	the apparent lactose content	1556:1583	the apparent lactose content	1556:1583	The results showed that the addition of protein-rich adulterants, as well as dicyandiamide and melamine, increased the apparent protein content, while the addition of carbohydrate-based adulterants, whey protein isolate, and skimmed milk powder, increased the apparent lactose content.
32846598	2	36	theme	apparent	374:381	arg1	parameters					397:406	the apparent compositional parameters	370:406	the apparent compositional parameters of milk from the Dutch market	370:436	The current study explores the impact of common milk adulterants on the apparent compositional parameters of milk from the Dutch market as measured by standardized Fourier transform infrared (FTIR) spectroscopy.
32846598	4	37	theme	adulteration	857:868	arg1	detection					844:852	detection	844:852	detection of adulteration	844:868	In this study we used measured boundaries but also more practical variance-adjusted boundaries to set thresholds for detection of adulteration.
32846598	4	38	theme	measured	749:756	arg1	boundaries					758:767	boundaries	758:767	measured boundaries but also more practical variance-adjusted boundaries	749:820	In this study we used measured boundaries but also more practical variance-adjusted boundaries to set thresholds for detection of adulteration.
32846598	1	39	theme	milk	290:293	arg1	fraud					295:299	milk fraud	290:299	milk fraud	290:299	Milk is regarded as one of the top food products susceptible to adulteration where its valuable components are specifically identified as high-risk indicators for milk fraud.
32846598	6	40	theme	concentration	1070:1082	arg1	levels					1084:1089	four concentration levels	1065:1089	four concentration levels	1065:1089	Twenty-four substances were used to produce various categories of milk adulterations, each at four concentration levels.
32846598	3	41	from	levels	606:611	arg1	detectability					546:558	the detectability	542:558	the detectability of these adulterants at various concentration levels using the compositional parameters individually, in a univariate manner, and together in a multivariate approach	542:724	More precisely, it examines the detectability of these adulterants at various concentration levels using the compositional parameters individually, in a univariate manner, and together in a multivariate approach.
32846598	2	42	theme	milk	411:414	arg1	parameters					397:406	the apparent compositional parameters	370:406	the apparent compositional parameters of milk from the Dutch market	370:436	The current study explores the impact of common milk adulterants on the apparent compositional parameters of milk from the Dutch market as measured by standardized Fourier transform infrared (FTIR) spectroscopy.
32846598	12	43	theme	protein	1955:1961	arg1	profit					1921:1926	the potential profit	1907:1926	the potential profit of milk adulteration, whey protein and corn starch	1907:1977	Regarding the potential profit of milk adulteration, whey protein and corn starch seem particularly interesting.
32846598	9	44	theme	protein-	1657:1664	arg1	adulterants					1685:1695	especially protein- and nitrogen-based adulterants	1646:1695	especially protein- and nitrogen-based adulterants	1646:1695	When considering the compositional parameters univariately, especially protein- and nitrogen-based adulterants did not raise a flag of unusual apparent concentrations at lower concentration levels.
32846598	2	45	theme	standardized	453:464	arg1	Fourier					466:472	standardized Fourier	453:472	standardized Fourier transform infrared (FTIR) spectroscopy	453:511	The current study explores the impact of common milk adulterants on the apparent compositional parameters of milk from the Dutch market as measured by standardized Fourier transform infrared (FTIR) spectroscopy.
32846598	13	46	theme	gaps	2109:2112	arg1	inflation					2035:2043	the artificial inflation	2020:2043	the artificial inflation of valuable components, the resulting potential profit, and the gaps in detection	2020:2125	Combining the artificial inflation of valuable components, the resulting potential profit, and the gaps in detection, it appears that the whey protein isolates deserve particular attention when thinking like a criminal.
32846598	7	47	theme	carbohydrate-based	1189:1206	arg1	adulterants					1208:1218	seven carbohydrate-based adulterants	1183:1218	seven carbohydrate-based adulterants	1183:1218	These substances comprised five protein-rich adulterants, five nitrogen-based adulterants, seven carbohydrate-based adulterants, six preservatives and water, resulting in a set of 360 samples to be analysed.
32846598	2	48	dep	infrared	484:491	arg1	FTIR					494:497	FTIR	494:497	FTIR	494:497	The current study explores the impact of common milk adulterants on the apparent compositional parameters of milk from the Dutch market as measured by standardized Fourier transform infrared (FTIR) spectroscopy.
32846598	9	49	theme	nitrogen-based	1670:1683	arg1	adulterants					1685:1695	especially protein- and nitrogen-based adulterants	1646:1695	especially protein- and nitrogen-based adulterants	1646:1695	When considering the compositional parameters univariately, especially protein- and nitrogen-based adulterants did not raise a flag of unusual apparent concentrations at lower concentration levels.
32846598	2	50	from	parameters	397:406	arg1	market					431:436	the Dutch market	421:436	the Dutch market	421:436	The current study explores the impact of common milk adulterants on the apparent compositional parameters of milk from the Dutch market as measured by standardized Fourier transform infrared (FTIR) spectroscopy.
32846598	4	51	theme	variance-adjusted	793:809	arg1	boundaries					811:820	more practical variance-adjusted boundaries	778:820	measured boundaries but also more practical variance-adjusted boundaries	749:820	In this study we used measured boundaries but also more practical variance-adjusted boundaries to set thresholds for detection of adulteration.
32846598	12	52	theme	milk	1931:1934	arg1	adulteration					1936:1947	milk adulteration	1931:1947	milk adulteration	1931:1947	Regarding the potential profit of milk adulteration, whey protein and corn starch seem particularly interesting.
32846598	8	53	theme	carbohydrate-based	1467:1484	arg1	adulterants					1486:1496	carbohydrate-based adulterants	1467:1496	carbohydrate-based adulterants	1467:1496	The results showed that the addition of protein-rich adulterants, as well as dicyandiamide and melamine, increased the apparent protein content, while the addition of carbohydrate-based adulterants, whey protein isolate, and skimmed milk powder, increased the apparent lactose content.
32846598	13	54	theme	profit	2093:2098	arg1	inflation					2035:2043	the artificial inflation	2020:2043	the artificial inflation of valuable components, the resulting potential profit, and the gaps in detection	2020:2125	Combining the artificial inflation of valuable components, the resulting potential profit, and the gaps in detection, it appears that the whey protein isolates deserve particular attention when thinking like a criminal.
32846598	1	55	theme	top	158:160	arg1	products					167:174	the top food products	154:174	the top food products susceptible to adulteration where its valuable components are specifically identified as high-risk indicators for milk fraud	154:299	Milk is regarded as one of the top food products susceptible to adulteration where its valuable components are specifically identified as high-risk indicators for milk fraud.
32846598	3	56	theme	multivariate	704:715	arg1	approach					717:724	a multivariate approach	702:724	a multivariate approach	702:724	More precisely, it examines the detectability of these adulterants at various concentration levels using the compositional parameters individually, in a univariate manner, and together in a multivariate approach.
32846598	2	57	theme	milk	350:353	arg1	adulterants					355:365	common milk adulterants	343:365	common milk adulterants	343:365	The current study explores the impact of common milk adulterants on the apparent compositional parameters of milk from the Dutch market as measured by standardized Fourier transform infrared (FTIR) spectroscopy.
32846598	9	58	theme	unusual	1721:1727	arg1	concentrations					1738:1751	unusual apparent concentrations	1721:1751	unusual apparent concentrations at lower concentration levels	1721:1781	When considering the compositional parameters univariately, especially protein- and nitrogen-based adulterants did not raise a flag of unusual apparent concentrations at lower concentration levels.
32846598	13	59	theme	resulting	2073:2081	arg1	profit					2093:2098	the resulting potential profit	2069:2098	the resulting potential profit	2069:2098	Combining the artificial inflation of valuable components, the resulting potential profit, and the gaps in detection, it appears that the whey protein isolates deserve particular attention when thinking like a criminal.
32846598	5	60	theme	potential	875:883	arg1	impact					894:899	The potential economic impact	871:899	The potential economic impact of these adulterations under a milk payment scheme	871:950	The potential economic impact of these adulterations under a milk payment scheme is also evaluated.
32846598	0	61	theme	profitable	41:50	arg1	adulterations					57:69	profitable milk adulterations	41:69	profitable milk adulterations	41:69	Opportunities for fraudsters: When would profitable milk adulterations go unnoticed by common, standardized FTIR measurements?
32846598	9	62	theme	concentrations	1738:1751	arg1	flag					1713:1716	a flag	1711:1716	a flag of unusual apparent concentrations at lower concentration levels	1711:1781	When considering the compositional parameters univariately, especially protein- and nitrogen-based adulterants did not raise a flag of unusual apparent concentrations at lower concentration levels.
32846598	1	63	theme	food	162:165	arg1	products					167:174	the top food products	154:174	the top food products susceptible to adulteration where its valuable components are specifically identified as high-risk indicators for milk fraud	154:299	Milk is regarded as one of the top food products susceptible to adulteration where its valuable components are specifically identified as high-risk indicators for milk fraud.
32846598	9	64	theme	lower	1756:1760	arg1	levels					1776:1781	lower concentration levels	1756:1781	lower concentration levels	1756:1781	When considering the compositional parameters univariately, especially protein- and nitrogen-based adulterants did not raise a flag of unusual apparent concentrations at lower concentration levels.
32846598	11	65	theme	detection	1886:1894	arg1	level					1877:1881	the level	1873:1881	the level of detection	1873:1894	The multivariate approach did not improve the level of detection.
32846598	13	66	theme	valuable	2048:2055	arg1	components					2057:2066	valuable components	2048:2066	valuable components	2048:2066	Combining the artificial inflation of valuable components, the resulting potential profit, and the gaps in detection, it appears that the whey protein isolates deserve particular attention when thinking like a criminal.
32846598	1	67	theme	susceptible	176:186	arg1	products					167:174	the top food products	154:174	the top food products susceptible to adulteration where its valuable components are specifically identified as high-risk indicators for milk fraud	154:299	Milk is regarded as one of the top food products susceptible to adulteration where its valuable components are specifically identified as high-risk indicators for milk fraud.
32846598	2	68	theme	current	306:312	arg1	study					314:318	The current study	302:318	The current study	302:318	The current study explores the impact of common milk adulterants on the apparent compositional parameters of milk from the Dutch market as measured by standardized Fourier transform infrared (FTIR) spectroscopy.
32846598	12	69	theme	potential	1911:1919	arg1	profit					1921:1926	the potential profit	1907:1926	the potential profit of milk adulteration, whey protein and corn starch	1907:1977	Regarding the potential profit of milk adulteration, whey protein and corn starch seem particularly interesting.
32846598	3	70	theme	concentration	592:604	arg1	levels					606:611	various concentration levels	584:611	various concentration levels using the compositional parameters individually, in a univariate manner, and together in a multivariate approach	584:724	More precisely, it examines the detectability of these adulterants at various concentration levels using the compositional parameters individually, in a univariate manner, and together in a multivariate approach.
32846598	13	71	from	profit	2093:2098	arg1	detection					2117:2125	detection	2117:2125	detection	2117:2125	Combining the artificial inflation of valuable components, the resulting potential profit, and the gaps in detection, it appears that the whey protein isolates deserve particular attention when thinking like a criminal.
32846598	8	72	theme	protein	1504:1510	arg1	isolate					1512:1518	whey protein isolate	1499:1518	whey protein isolate	1499:1518	The results showed that the addition of protein-rich adulterants, as well as dicyandiamide and melamine, increased the apparent protein content, while the addition of carbohydrate-based adulterants, whey protein isolate, and skimmed milk powder, increased the apparent lactose content.
32846598	5	73	theme	milk	932:935	arg1	scheme					945:950	a milk payment scheme	930:950	a milk payment scheme	930:950	The potential economic impact of these adulterations under a milk payment scheme is also evaluated.
32846598	6	74	used	used	999:1002	arg2	substances					983:992	Twenty-four substances	971:992	Twenty-four substances	971:992	Twenty-four substances were used to produce various categories of milk adulterations, each at four concentration levels.
32846598	13	75	theme	particular	2178:2187	arg1	attention					2189:2197	particular attention	2178:2197	particular attention	2178:2197	Combining the artificial inflation of valuable components, the resulting potential profit, and the gaps in detection, it appears that the whey protein isolates deserve particular attention when thinking like a criminal.
32846598	3	76	theme	compositional	623:635	arg1	parameters					637:646	the compositional parameters	619:646	the compositional parameters individually, in a univariate manner, and together in a multivariate approach	619:724	More precisely, it examines the detectability of these adulterants at various concentration levels using the compositional parameters individually, in a univariate manner, and together in a multivariate approach.
32846598	8	77	theme	skimmed	1525:1531	arg1	powder					1538:1543	skimmed milk powder	1525:1543	skimmed milk powder	1525:1543	The results showed that the addition of protein-rich adulterants, as well as dicyandiamide and melamine, increased the apparent protein content, while the addition of carbohydrate-based adulterants, whey protein isolate, and skimmed milk powder, increased the apparent lactose content.
32846598	11	78	theme	multivariate	1835:1846	arg1	approach					1848:1855	The multivariate approach	1831:1855	The multivariate approach	1831:1855	The multivariate approach did not improve the level of detection.
32846598	6	79	theme	adulterations	1042:1054	arg1	categories					1023:1032	various categories	1015:1032	various categories of milk adulterations	1015:1054	Twenty-four substances were used to produce various categories of milk adulterations, each at four concentration levels.
32846598	6	79	theme	adulterations	1042:1054	arg1	each					1057:1060	each	1057:1060	each	1057:1060	Twenty-four substances were used to produce various categories of milk adulterations, each at four concentration levels.
32846598	8	80	theme	powder	1538:1543	arg1	addition					1455:1462	the addition	1451:1462	the addition of carbohydrate-based adulterants, whey protein isolate, and skimmed milk powder,	1451:1544	The results showed that the addition of protein-rich adulterants, as well as dicyandiamide and melamine, increased the apparent protein content, while the addition of carbohydrate-based adulterants, whey protein isolate, and skimmed milk powder, increased the apparent lactose content.
32846598	8	81	theme	adulterants	1353:1363	arg1	addition					1328:1335	the addition	1324:1335	the addition of protein-rich adulterants, as well as dicyandiamide and melamine,	1324:1403	The results showed that the addition of protein-rich adulterants, as well as dicyandiamide and melamine, increased the apparent protein content, while the addition of carbohydrate-based adulterants, whey protein isolate, and skimmed milk powder, increased the apparent lactose content.
32846598	13	82	theme	whey	2148:2151	arg1	isolates					2161:2168	the whey protein isolates	2144:2168	the whey protein isolates	2144:2168	Combining the artificial inflation of valuable components, the resulting potential profit, and the gaps in detection, it appears that the whey protein isolates deserve particular attention when thinking like a criminal.
32846598	13	83	from	components	2057:2066	arg1	detection					2117:2125	detection	2117:2125	detection	2117:2125	Combining the artificial inflation of valuable components, the resulting potential profit, and the gaps in detection, it appears that the whey protein isolates deserve particular attention when thinking like a criminal.
32846598	2	84	theme	infrared	484:491	arg1	spectroscopy					500:511	infrared (FTIR) spectroscopy	484:511	infrared (FTIR) spectroscopy	484:511	The current study explores the impact of common milk adulterants on the apparent compositional parameters of milk from the Dutch market as measured by standardized Fourier transform infrared (FTIR) spectroscopy.
32846598	6	85	theme	various	1015:1021	arg1	categories					1023:1032	various categories	1015:1032	various categories of milk adulterations	1015:1054	Twenty-four substances were used to produce various categories of milk adulterations, each at four concentration levels.
32846598	6	85	theme	various	1015:1021	arg1	each					1057:1060	each	1057:1060	each	1057:1060	Twenty-four substances were used to produce various categories of milk adulterations, each at four concentration levels.
32846598	8	86	theme	apparent	1560:1567	arg1	content					1577:1583	the apparent lactose content	1556:1583	the apparent lactose content	1556:1583	The results showed that the addition of protein-rich adulterants, as well as dicyandiamide and melamine, increased the apparent protein content, while the addition of carbohydrate-based adulterants, whey protein isolate, and skimmed milk powder, increased the apparent lactose content.
32846598	0	87	theme	FTIR	108:111	arg1	measurements					113:124	common, standardized FTIR measurements	87:124	common, standardized FTIR measurements	87:124	Opportunities for fraudsters: When would profitable milk adulterations go unnoticed by common, standardized FTIR measurements?
32846598	12	88	theme	starch	1972:1977	arg1	profit					1921:1926	the potential profit	1907:1926	the potential profit of milk adulteration, whey protein and corn starch	1907:1977	Regarding the potential profit of milk adulteration, whey protein and corn starch seem particularly interesting.
32846598	2	89	theme	compositional	383:395	arg1	parameters					397:406	the apparent compositional parameters	370:406	the apparent compositional parameters of milk from the Dutch market	370:436	The current study explores the impact of common milk adulterants on the apparent compositional parameters of milk from the Dutch market as measured by standardized Fourier transform infrared (FTIR) spectroscopy.
32846598	9	90	theme	compositional	1607:1619	arg1	parameters					1621:1630	the compositional parameters	1603:1630	the compositional parameters	1603:1630	When considering the compositional parameters univariately, especially protein- and nitrogen-based adulterants did not raise a flag of unusual apparent concentrations at lower concentration levels.
32846598	12	91	theme	whey	1950:1953	arg1	protein					1955:1961	whey protein	1950:1961	whey protein	1950:1961	Regarding the potential profit of milk adulteration, whey protein and corn starch seem particularly interesting.
32846598	2	92	from	market	431:436	arg1	milk					411:414	milk	411:414	milk from the Dutch market	411:436	The current study explores the impact of common milk adulterants on the apparent compositional parameters of milk from the Dutch market as measured by standardized Fourier transform infrared (FTIR) spectroscopy.
32846598	2	92	from	market	431:436	arg1	parameters					397:406	the apparent compositional parameters	370:406	the apparent compositional parameters of milk from the Dutch market	370:436	The current study explores the impact of common milk adulterants on the apparent compositional parameters of milk from the Dutch market as measured by standardized Fourier transform infrared (FTIR) spectroscopy.
32846598	2	93	theme	Dutch	425:429	arg1	market					431:436	the Dutch market	421:436	the Dutch market	421:436	The current study explores the impact of common milk adulterants on the apparent compositional parameters of milk from the Dutch market as measured by standardized Fourier transform infrared (FTIR) spectroscopy.
32846598	8	94	theme	protein	1428:1434	arg1	content					1436:1442	the apparent protein content	1415:1442	the apparent protein content	1415:1442	The results showed that the addition of protein-rich adulterants, as well as dicyandiamide and melamine, increased the apparent protein content, while the addition of carbohydrate-based adulterants, whey protein isolate, and skimmed milk powder, increased the apparent lactose content.
32846598	0	95	theme	milk	52:55	arg1	adulterations					57:69	profitable milk adulterations	41:69	profitable milk adulterations	41:69	Opportunities for fraudsters: When would profitable milk adulterations go unnoticed by common, standardized FTIR measurements?
32846598	12	96	theme	adulteration	1936:1947	arg1	profit					1921:1926	the potential profit	1907:1926	the potential profit of milk adulteration, whey protein and corn starch	1907:1977	Regarding the potential profit of milk adulteration, whey protein and corn starch seem particularly interesting.
32846598	3	97	theme	univariate	667:676	arg1	manner					678:683	a univariate manner	665:683	a univariate manner	665:683	More precisely, it examines the detectability of these adulterants at various concentration levels using the compositional parameters individually, in a univariate manner, and together in a multivariate approach.
32846598	4	98	theme	practical	783:791	arg1	boundaries					811:820	more practical variance-adjusted boundaries	778:820	measured boundaries but also more practical variance-adjusted boundaries	749:820	In this study we used measured boundaries but also more practical variance-adjusted boundaries to set thresholds for detection of adulteration.
32846598	13	99	theme	potential	2083:2091	arg1	profit					2093:2098	the resulting potential profit	2069:2098	the resulting potential profit	2069:2098	Combining the artificial inflation of valuable components, the resulting potential profit, and the gaps in detection, it appears that the whey protein isolates deserve particular attention when thinking like a criminal.
32846598	1	100	theme	valuable	214:221	arg1	components					223:232	its valuable components	210:232	its valuable components	210:232	Milk is regarded as one of the top food products susceptible to adulteration where its valuable components are specifically identified as high-risk indicators for milk fraud.
32846598	1	100	theme	valuable	214:221	arg1	indicators					275:284	high-risk indicators	265:284	high-risk indicators for milk fraud	265:299	Milk is regarded as one of the top food products susceptible to adulteration where its valuable components are specifically identified as high-risk indicators for milk fraud.
32846598	2	101	theme	adulterants	355:365	arg1	impact					333:338	the impact	329:338	the impact of common milk adulterants on the apparent compositional parameters of milk from the Dutch market	329:436	The current study explores the impact of common milk adulterants on the apparent compositional parameters of milk from the Dutch market as measured by standardized Fourier transform infrared (FTIR) spectroscopy.
32846598	4	102	used	used	744:747	arg2	we					741:742	we	741:742	we	741:742	In this study we used measured boundaries but also more practical variance-adjusted boundaries to set thresholds for detection of adulteration.
33115651	4	0	theme	moisture	740:747	arg1	capacity					760:767	moisture absorption capacity	740:767	moisture absorption capacity	740:767	The values of water contact angle for films (53°) and nonwoven mats (50.6°) suggested that chitosan enhanced hydrophilic properties and moisture absorption capacity of the composite materials.
33115651	3	1	theme	surface	466:472	arg1	morphology					474:483	the surface morphology	462:483	the surface morphology of the composite films	462:506	Scanning electron microscopy showed that addition of chitosan induced changes in the surface morphology of the composite films and a reduction in the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm.
33115651	5	2	theme	reinforcing	834:844	arg1	ability					846:852	its reinforcing ability	830:852	its reinforcing ability	830:852	On the other hand, P(3HB) showed its reinforcing ability and improved the physical/mechanical properties of chitosan.
33115651	6	3	dep	cell	1025:1028	arg1	growth					1030:1035	growth	1030:1035	growth	1030:1035	The work included studies of in vitro biodegradation of the composite specimens and their ability to maintain cell growth and attachment in NIH 3T3 fibroblast culture.
33115651	3	4	theme	films	502:506	arg1	morphology					474:483	the surface morphology	462:483	the surface morphology of the composite films	462:506	Scanning electron microscopy showed that addition of chitosan induced changes in the surface morphology of the composite films and a reduction in the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm.
33115651	6	5	theme	NIH	1055:1057	arg1	3T3					1059:1061	NIH 3T3	1055:1061	NIH 3T3 fibroblast culture	1055:1080	The work included studies of in vitro biodegradation of the composite specimens and their ability to maintain cell growth and attachment in NIH 3T3 fibroblast culture.
33115651	4	6	theme	nonwoven	658:665	arg1	mats					667:670	nonwoven mats	658:670	nonwoven mats (50.6°)	658:678	The values of water contact angle for films (53°) and nonwoven mats (50.6°) suggested that chitosan enhanced hydrophilic properties and moisture absorption capacity of the composite materials.
33115651	4	6	theme	nonwoven	658:665	arg1	50.6°					673:677	50.6°	673:677	50.6°	673:677	The values of water contact angle for films (53°) and nonwoven mats (50.6°) suggested that chitosan enhanced hydrophilic properties and moisture absorption capacity of the composite materials.
33115651	5	7	theme	physical/mechanical	871:889	arg1	properties					891:900	the physical/mechanical properties	867:900	the physical/mechanical properties of chitosan	867:912	On the other hand, P(3HB) showed its reinforcing ability and improved the physical/mechanical properties of chitosan.
33115651	1	8	theme	nonwoven	87:94	arg1	mats					96:99	nonwoven mats	87:99	nonwoven mats	87:99	Composite films and nonwoven mats of the poly-3-hydroxybutyrate and chitosan natural polymers were prepared and investigated.
33115651	3	9	theme	electron	390:397	arg1	microscopy					399:408	Scanning electron microscopy	381:408	Scanning electron microscopy	381:408	Scanning electron microscopy showed that addition of chitosan induced changes in the surface morphology of the composite films and a reduction in the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm.
33115651	3	10	from	460 nm	596:601	arg1	diameter					531:538	the diameter	527:538	the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm	527:601	Scanning electron microscopy showed that addition of chitosan induced changes in the surface morphology of the composite films and a reduction in the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm.
33115651	3	10	from	460 nm	596:601	arg1	mats					576:579	the nonwoven mats	563:579	the nonwoven mats from 800 nm to 460 nm	563:601	Scanning electron microscopy showed that addition of chitosan induced changes in the surface morphology of the composite films and a reduction in the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm.
33115651	6	11	dep	in	944:945	arg1	vitro					947:951	vitro	947:951	vitro	947:951	The work included studies of in vitro biodegradation of the composite specimens and their ability to maintain cell growth and attachment in NIH 3T3 fibroblast culture.
33115651	6	12	theme	3T3	1059:1061	arg1	culture					1074:1080	NIH 3T3 fibroblast culture	1055:1080	NIH 3T3 fibroblast culture	1055:1080	The work included studies of in vitro biodegradation of the composite specimens and their ability to maintain cell growth and attachment in NIH 3T3 fibroblast culture.
33115651	3	13	theme	composite	492:500	arg1	films					502:506	the composite films	488:506	the composite films	488:506	Scanning electron microscopy showed that addition of chitosan induced changes in the surface morphology of the composite films and a reduction in the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm.
33115651	6	14	theme	ability	1005:1011	arg1	studies					933:939	studies	933:939	studies of in vitro biodegradation of the composite specimens and their ability to maintain cell growth and attachment in NIH 3T3 fibroblast culture	933:1080	The work included studies of in vitro biodegradation of the composite specimens and their ability to maintain cell growth and attachment in NIH 3T3 fibroblast culture.
33115651	0	15	theme	composite	32:40	arg1	films					42:46	Poly-3-hydroxybutyrate/chitosan composite films	0:46	Poly-3-hydroxybutyrate/chitosan composite films	0:46	Poly-3-hydroxybutyrate/chitosan composite films and nonwoven mats.
33115651	4	16	theme	materials	786:794	arg1	properties					725:734	hydrophilic properties	713:734	hydrophilic properties	713:734	The values of water contact angle for films (53°) and nonwoven mats (50.6°) suggested that chitosan enhanced hydrophilic properties and moisture absorption capacity of the composite materials.
33115651	4	16	theme	materials	786:794	arg1	capacity					760:767	moisture absorption capacity	740:767	moisture absorption capacity	740:767	The values of water contact angle for films (53°) and nonwoven mats (50.6°) suggested that chitosan enhanced hydrophilic properties and moisture absorption capacity of the composite materials.
33115651	2	17	from	%	325:325	arg1	films					342:346	the films	338:346	the films	338:346	DSC and FTIR techniques were used to confirm that P(3HB) blending with chitosan resulted in a decrease in P(3HB) crystallinity to 47% and 62% in the films and nonwoven mats, respectively.
33115651	2	18	theme	3HB	301:303	arg1	crystallinity					306:318	P(3HB) crystallinity	299:318	P(3HB) crystallinity to 47% and 62% in the films and nonwoven mats, respectively	299:378	DSC and FTIR techniques were used to confirm that P(3HB) blending with chitosan resulted in a decrease in P(3HB) crystallinity to 47% and 62% in the films and nonwoven mats, respectively.
33115651	6	19	theme	specimens	985:993	arg1	ability					1005:1011	their ability to maintain cell growth and attachment in NIH 3T3 fibroblast culture	999:1080	their ability to maintain cell growth and attachment in NIH 3T3 fibroblast culture	999:1080	The work included studies of in vitro biodegradation of the composite specimens and their ability to maintain cell growth and attachment in NIH 3T3 fibroblast culture.
33115651	6	19	theme	specimens	985:993	arg1	biodegradation					953:966	in vitro biodegradation	944:966	in vitro biodegradation of the composite specimens	944:993	The work included studies of in vitro biodegradation of the composite specimens and their ability to maintain cell growth and attachment in NIH 3T3 fibroblast culture.
33115651	0	20	theme	Poly-3-hydroxybutyrate/chitosan	0:30	arg1	films					42:46	Poly-3-hydroxybutyrate/chitosan composite films	0:46	Poly-3-hydroxybutyrate/chitosan composite films	0:46	Poly-3-hydroxybutyrate/chitosan composite films and nonwoven mats.
33115651	4	21	theme	composite	776:784	arg1	materials					786:794	the composite materials	772:794	the composite materials	772:794	The values of water contact angle for films (53°) and nonwoven mats (50.6°) suggested that chitosan enhanced hydrophilic properties and moisture absorption capacity of the composite materials.
33115651	2	22	from	%	333:333	arg1	films					342:346	the films	338:346	the films	338:346	DSC and FTIR techniques were used to confirm that P(3HB) blending with chitosan resulted in a decrease in P(3HB) crystallinity to 47% and 62% in the films and nonwoven mats, respectively.
33115651	6	23	theme	fibroblast	1063:1072	arg1	culture					1074:1080	NIH 3T3 fibroblast culture	1055:1080	NIH 3T3 fibroblast culture	1055:1080	The work included studies of in vitro biodegradation of the composite specimens and their ability to maintain cell growth and attachment in NIH 3T3 fibroblast culture.
33115651	3	24	from	diameter	531:538	arg1	460 nm					596:601	460 nm	596:601	460 nm	596:601	Scanning electron microscopy showed that addition of chitosan induced changes in the surface morphology of the composite films and a reduction in the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm.
33115651	3	24	from	diameter	531:538	arg1	mats					576:579	the nonwoven mats	563:579	the nonwoven mats from 800 nm to 460 nm	563:601	Scanning electron microscopy showed that addition of chitosan induced changes in the surface morphology of the composite films and a reduction in the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm.
33115651	3	25	theme	Scanning	381:388	arg1	microscopy					399:408	Scanning electron microscopy	381:408	Scanning electron microscopy	381:408	Scanning electron microscopy showed that addition of chitosan induced changes in the surface morphology of the composite films and a reduction in the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm.
33115651	3	26	from	changes	451:457	arg1	diameter					531:538	the diameter	527:538	the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm	527:601	Scanning electron microscopy showed that addition of chitosan induced changes in the surface morphology of the composite films and a reduction in the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm.
33115651	3	26	from	changes	451:457	arg1	morphology					474:483	the surface morphology	462:483	the surface morphology of the composite films	462:506	Scanning electron microscopy showed that addition of chitosan induced changes in the surface morphology of the composite films and a reduction in the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm.
33115651	2	27	theme	P	299:299	arg1	crystallinity					306:318	P(3HB) crystallinity	299:318	P(3HB) crystallinity to 47% and 62% in the films and nonwoven mats, respectively	299:378	DSC and FTIR techniques were used to confirm that P(3HB) blending with chitosan resulted in a decrease in P(3HB) crystallinity to 47% and 62% in the films and nonwoven mats, respectively.
33115651	3	28	theme	ultrafine	543:551	arg1	fibers					553:558	ultrafine fibers	543:558	ultrafine fibers	543:558	Scanning electron microscopy showed that addition of chitosan induced changes in the surface morphology of the composite films and a reduction in the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm.
33115651	4	29	theme	angle	632:636	arg1	values					608:613	The values	604:613	The values of water contact angle for films (53°) and nonwoven mats (50.6°)	604:678	The values of water contact angle for films (53°) and nonwoven mats (50.6°) suggested that chitosan enhanced hydrophilic properties and moisture absorption capacity of the composite materials.
33115651	4	30	theme	contact	624:630	arg1	angle					632:636	water contact angle	618:636	water contact angle	618:636	The values of water contact angle for films (53°) and nonwoven mats (50.6°) suggested that chitosan enhanced hydrophilic properties and moisture absorption capacity of the composite materials.
33115651	2	31	theme	FTIR	201:204	arg1	techniques					206:215	DSC and FTIR techniques	193:215	techniques	206:215	DSC and FTIR techniques were used to confirm that P(3HB) blending with chitosan resulted in a decrease in P(3HB) crystallinity to 47% and 62% in the films and nonwoven mats, respectively.
33115651	2	32	theme	3HB	245:247	arg1	blending					250:257	P(3HB) blending	243:257	P(3HB) blending with chitosan	243:271	DSC and FTIR techniques were used to confirm that P(3HB) blending with chitosan resulted in a decrease in P(3HB) crystallinity to 47% and 62% in the films and nonwoven mats, respectively.
33115651	5	33	theme	other	804:808	arg1	hand					810:813	the other hand	800:813	the other hand	800:813	On the other hand, P(3HB) showed its reinforcing ability and improved the physical/mechanical properties of chitosan.
33115651	6	34	theme	biodegradation	953:966	arg1	studies					933:939	studies	933:939	studies of in vitro biodegradation of the composite specimens and their ability to maintain cell growth and attachment in NIH 3T3 fibroblast culture	933:1080	The work included studies of in vitro biodegradation of the composite specimens and their ability to maintain cell growth and attachment in NIH 3T3 fibroblast culture.
33115651	0	35	theme	nonwoven	52:59	arg1	mats					61:64	nonwoven mats	52:64	nonwoven mats	52:64	Poly-3-hydroxybutyrate/chitosan composite films and nonwoven mats.
33115651	2	36	with	blending	250:257	arg1	chitosan					264:271	chitosan	264:271	chitosan	264:271	DSC and FTIR techniques were used to confirm that P(3HB) blending with chitosan resulted in a decrease in P(3HB) crystallinity to 47% and 62% in the films and nonwoven mats, respectively.
33115651	3	37	theme	fibers	553:558	arg1	diameter					531:538	the diameter	527:538	the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm	527:601	Scanning electron microscopy showed that addition of chitosan induced changes in the surface morphology of the composite films and a reduction in the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm.
33115651	2	38	theme	nonwoven	352:359	arg1	mats					361:364	nonwoven mats	352:364	nonwoven mats	352:364	DSC and FTIR techniques were used to confirm that P(3HB) blending with chitosan resulted in a decrease in P(3HB) crystallinity to 47% and 62% in the films and nonwoven mats, respectively.
33115651	2	39	theme	DSC	193:195	arg1	techniques					206:215	DSC and FTIR techniques	193:215	techniques	206:215	DSC and FTIR techniques were used to confirm that P(3HB) blending with chitosan resulted in a decrease in P(3HB) crystallinity to 47% and 62% in the films and nonwoven mats, respectively.
33115651	2	40	theme	P	243:243	arg1	blending					250:257	P(3HB) blending	243:257	P(3HB) blending with chitosan	243:271	DSC and FTIR techniques were used to confirm that P(3HB) blending with chitosan resulted in a decrease in P(3HB) crystallinity to 47% and 62% in the films and nonwoven mats, respectively.
33115651	1	41	theme	chitosan	135:142	arg1	films					77:81	Composite films	67:81	Composite films	67:81	Composite films and nonwoven mats of the poly-3-hydroxybutyrate and chitosan natural polymers were prepared and investigated.
33115651	1	41	theme	chitosan	135:142	arg1	mats					96:99	nonwoven mats	87:99	nonwoven mats	87:99	Composite films and nonwoven mats of the poly-3-hydroxybutyrate and chitosan natural polymers were prepared and investigated.
33115651	4	42	theme	absorption	749:758	arg1	capacity					760:767	moisture absorption capacity	740:767	moisture absorption capacity	740:767	The values of water contact angle for films (53°) and nonwoven mats (50.6°) suggested that chitosan enhanced hydrophilic properties and moisture absorption capacity of the composite materials.
33115651	1	43	theme	natural	144:150	arg1	polymers					152:159	natural polymers	144:159	natural polymers	144:159	Composite films and nonwoven mats of the poly-3-hydroxybutyrate and chitosan natural polymers were prepared and investigated.
33115651	4	44	theme	hydrophilic	713:723	arg1	properties					725:734	hydrophilic properties	713:734	hydrophilic properties	713:734	The values of water contact angle for films (53°) and nonwoven mats (50.6°) suggested that chitosan enhanced hydrophilic properties and moisture absorption capacity of the composite materials.
33115651	5	45	theme	chitosan	905:912	arg1	properties					891:900	the physical/mechanical properties	867:900	the physical/mechanical properties of chitosan	867:912	On the other hand, P(3HB) showed its reinforcing ability and improved the physical/mechanical properties of chitosan.
33115651	3	46	theme	nonwoven	567:574	arg1	mats					576:579	the nonwoven mats	563:579	the nonwoven mats from 800 nm to 460 nm	563:601	Scanning electron microscopy showed that addition of chitosan induced changes in the surface morphology of the composite films and a reduction in the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm.
33115651	3	47	theme	chitosan	434:441	arg1	addition					422:429	addition	422:429	addition of chitosan	422:441	Scanning electron microscopy showed that addition of chitosan induced changes in the surface morphology of the composite films and a reduction in the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm.
33115651	1	48	theme	poly-3-hydroxybutyrate	108:129	arg1	films					77:81	Composite films	67:81	Composite films	67:81	Composite films and nonwoven mats of the poly-3-hydroxybutyrate and chitosan natural polymers were prepared and investigated.
33115651	1	48	theme	poly-3-hydroxybutyrate	108:129	arg1	mats					96:99	nonwoven mats	87:99	nonwoven mats	87:99	Composite films and nonwoven mats of the poly-3-hydroxybutyrate and chitosan natural polymers were prepared and investigated.
33115651	6	49	theme	in	944:945	arg1	biodegradation					953:966	in vitro biodegradation	944:966	in vitro biodegradation of the composite specimens	944:993	The work included studies of in vitro biodegradation of the composite specimens and their ability to maintain cell growth and attachment in NIH 3T3 fibroblast culture.
33115651	6	50	theme	composite	975:983	arg1	specimens					985:993	the composite specimens	971:993	the composite specimens	971:993	The work included studies of in vitro biodegradation of the composite specimens and their ability to maintain cell growth and attachment in NIH 3T3 fibroblast culture.
33115651	2	51	from	mats	361:364	arg1	films					342:346	the films	338:346	the films	338:346	DSC and FTIR techniques were used to confirm that P(3HB) blending with chitosan resulted in a decrease in P(3HB) crystallinity to 47% and 62% in the films and nonwoven mats, respectively.
33115651	2	52	from	decrease	287:294	arg1	crystallinity					306:318	P(3HB) crystallinity	299:318	P(3HB) crystallinity to 47% and 62% in the films and nonwoven mats, respectively	299:378	DSC and FTIR techniques were used to confirm that P(3HB) blending with chitosan resulted in a decrease in P(3HB) crystallinity to 47% and 62% in the films and nonwoven mats, respectively.
33115651	1	53	dep	poly-3-hydroxybutyrate	108:129	arg1	polymers					152:159	natural polymers	144:159	natural polymers	144:159	Composite films and nonwoven mats of the poly-3-hydroxybutyrate and chitosan natural polymers were prepared and investigated.
33115651	3	54	from	reduction	514:522	arg1	diameter					531:538	the diameter	527:538	the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm	527:601	Scanning electron microscopy showed that addition of chitosan induced changes in the surface morphology of the composite films and a reduction in the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm.
33115651	3	54	from	reduction	514:522	arg1	morphology					474:483	the surface morphology	462:483	the surface morphology of the composite films	462:506	Scanning electron microscopy showed that addition of chitosan induced changes in the surface morphology of the composite films and a reduction in the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm.
33115651	2	55	used	used	222:225	arg2	techniques					206:215	DSC and FTIR techniques	193:215	techniques	206:215	DSC and FTIR techniques were used to confirm that P(3HB) blending with chitosan resulted in a decrease in P(3HB) crystallinity to 47% and 62% in the films and nonwoven mats, respectively.
33115651	1	56	theme	Composite	67:75	arg1	films					77:81	Composite films	67:81	Composite films	67:81	Composite films and nonwoven mats of the poly-3-hydroxybutyrate and chitosan natural polymers were prepared and investigated.
33115651	4	57	theme	water	618:622	arg1	angle					632:636	water contact angle	618:636	water contact angle	618:636	The values of water contact angle for films (53°) and nonwoven mats (50.6°) suggested that chitosan enhanced hydrophilic properties and moisture absorption capacity of the composite materials.
33115651	3	58	dep	460 nm	596:601	arg1	to					593:594	to	593:594	to	593:594	Scanning electron microscopy showed that addition of chitosan induced changes in the surface morphology of the composite films and a reduction in the diameter of ultrafine fibers in the nonwoven mats from 800 nm to 460 nm.
33957063	7	0	theme	HPCH	1313:1316	arg1	hydrogel					1318:1325	HPCH hydrogel	1313:1325	HPCH hydrogel	1313:1325	Importantly, less burst drug release and long-term sustained-release from the CMCH-Ms/HPCH composite hydrogel was observed than those from only CMCH-Ms or HPCH hydrogel.
33957063	8	1	theme	composite	1338:1346	arg1	hydrogel					1361:1368	the composite CMCH-Ms/HPCH hydrogel	1334:1368	the composite CMCH-Ms/HPCH hydrogel	1334:1368	Thus, the composite CMCH-Ms/HPCH hydrogel exhibited great potential application for loading different drugs and sustained drug release in controlled local drug delivery systems.
33957063	4	2	theme	rheological	766:776	arg1	properties					793:802	rheological and mechanical properties	766:802	properties	793:802	The morphology, gelation rate, swelling, rheological and mechanical properties, in vitro degradation and cytotoxicity, drug loading and drug release of the CMCH-Ms/HPCH gel scaffolds were examined.
33957063	0	3	with	embedded	42:49	arg1	microspheres					77:88	carboxymethyl chitin microspheres	56:88	carboxymethyl chitin microspheres prepared via a solvent-free process for drug delivery	56:142	Injectable hydroxypropyl chitin hydrogels embedded with carboxymethyl chitin microspheres prepared via a solvent-free process for drug delivery.
33957063	2	4	theme	10-100 μm	384:392	arg1	diameter					372:379	a diameter	370:379	a diameter of 10-100 μm	370:392	In this study, we prepared carboxymethyl chitin microspheres (CMCH-Ms) with a diameter of 10-100 μm through physical crosslinking by increasing temperature in an aqueous two-phase system without using organic solvents, surfactants and crosslinking agents.
33957063	2	5	with	microspheres	342:353	arg1	diameter					372:379	a diameter	370:379	a diameter of 10-100 μm	370:392	In this study, we prepared carboxymethyl chitin microspheres (CMCH-Ms) with a diameter of 10-100 μm through physical crosslinking by increasing temperature in an aqueous two-phase system without using organic solvents, surfactants and crosslinking agents.
33957063	2	6	theme	crosslinking	529:540	arg1	agents					542:547	crosslinking agents	529:547	crosslinking agents	529:547	In this study, we prepared carboxymethyl chitin microspheres (CMCH-Ms) with a diameter of 10-100 μm through physical crosslinking by increasing temperature in an aqueous two-phase system without using organic solvents, surfactants and crosslinking agents.
33957063	6	7	used	used	1079:1082	arg2	solvent					1067:1073	no organic solvent	1056:1073	no organic solvent	1056:1073	Moreover, no organic solvent was used in the preparation and drug loading process of CMCH-Ms/HPCH gel scaffold.
33957063	5	8	theme	CMCH-Ms/HPCH	981:992	arg1	scaffolds					998:1006	CMCH-Ms/HPCH gel scaffolds	981:1006	CMCH-Ms/HPCH gel scaffolds	981:1006	In vitro degradation and cytotoxicity test indicated that CMCH-Ms/HPCH gel scaffolds were biodegradable and non-cytotoxic.
33957063	5	9	dep	In	923:924	arg1	vitro					926:930	vitro	926:930	vitro	926:930	In vitro degradation and cytotoxicity test indicated that CMCH-Ms/HPCH gel scaffolds were biodegradable and non-cytotoxic.
33957063	8	10	from	loading	1412:1418	arg1	systems					1497:1503	controlled local drug delivery systems	1466:1503	controlled local drug delivery systems	1466:1503	Thus, the composite CMCH-Ms/HPCH hydrogel exhibited great potential application for loading different drugs and sustained drug release in controlled local drug delivery systems.
33957063	2	11	theme	carboxymethyl	321:333	arg1	microspheres					342:353	carboxymethyl chitin microspheres	321:353	carboxymethyl chitin microspheres (CMCH-Ms) with a diameter of 10-100 μm	321:392	In this study, we prepared carboxymethyl chitin microspheres (CMCH-Ms) with a diameter of 10-100 μm through physical crosslinking by increasing temperature in an aqueous two-phase system without using organic solvents, surfactants and crosslinking agents.
33957063	2	11	theme	carboxymethyl	321:333	arg1	CMCH-Ms					356:362	CMCH-Ms	356:362	CMCH-Ms	356:362	In this study, we prepared carboxymethyl chitin microspheres (CMCH-Ms) with a diameter of 10-100 μm through physical crosslinking by increasing temperature in an aqueous two-phase system without using organic solvents, surfactants and crosslinking agents.
33957063	3	12	theme	different	628:636	arg1	environments					641:652	different pH environments	628:652	different pH environments	628:652	The stable microspheres keeping spherical shape with porous microstructure in different pH environments were embeded in thermosensitive hydroxypropyl chitin (HPCH) hydrogels.
33957063	0	13	theme	solvent-free	105:116	arg1	process					118:124	a solvent-free process	103:124	a solvent-free process for drug delivery	103:142	Injectable hydroxypropyl chitin hydrogels embedded with carboxymethyl chitin microspheres prepared via a solvent-free process for drug delivery.
33957063	7	14	theme	long-term	1199:1207	arg1	sustained-release					1209:1225	long-term sustained-release	1199:1225	long-term sustained-release	1199:1225	Importantly, less burst drug release and long-term sustained-release from the CMCH-Ms/HPCH composite hydrogel was observed than those from only CMCH-Ms or HPCH hydrogel.
33957063	3	15	theme	stable	554:559	arg1	microspheres					561:572	The stable microspheres	550:572	The stable microspheres keeping spherical shape with porous microstructure in different pH environments	550:652	The stable microspheres keeping spherical shape with porous microstructure in different pH environments were embeded in thermosensitive hydroxypropyl chitin (HPCH) hydrogels.
33957063	0	16	theme	drug	130:133	arg1	delivery					135:142	drug delivery	130:142	drug delivery	130:142	Injectable hydroxypropyl chitin hydrogels embedded with carboxymethyl chitin microspheres prepared via a solvent-free process for drug delivery.
33957063	5	17	theme	gel	994:996	arg1	scaffolds					998:1006	CMCH-Ms/HPCH gel scaffolds	981:1006	CMCH-Ms/HPCH gel scaffolds	981:1006	In vitro degradation and cytotoxicity test indicated that CMCH-Ms/HPCH gel scaffolds were biodegradable and non-cytotoxic.
33957063	7	18	theme	drug	1182:1185	arg1	release					1187:1193	burst drug release	1176:1193	burst drug release	1176:1193	Importantly, less burst drug release and long-term sustained-release from the CMCH-Ms/HPCH composite hydrogel was observed than those from only CMCH-Ms or HPCH hydrogel.
33957063	6	19	theme	CMCH-Ms/HPCH	1131:1142	arg1	scaffold					1148:1155	CMCH-Ms/HPCH gel scaffold	1131:1155	CMCH-Ms/HPCH gel scaffold	1131:1155	Moreover, no organic solvent was used in the preparation and drug loading process of CMCH-Ms/HPCH gel scaffold.
33957063	7	20	from	hydrogel	1259:1266	arg1	release					1187:1193	burst drug release	1176:1193	burst drug release	1176:1193	Importantly, less burst drug release and long-term sustained-release from the CMCH-Ms/HPCH composite hydrogel was observed than those from only CMCH-Ms or HPCH hydrogel.
33957063	7	20	from	hydrogel	1259:1266	arg1	sustained-release					1209:1225	long-term sustained-release	1199:1225	long-term sustained-release	1199:1225	Importantly, less burst drug release and long-term sustained-release from the CMCH-Ms/HPCH composite hydrogel was observed than those from only CMCH-Ms or HPCH hydrogel.
33957063	1	21	theme	natural	196:202	arg1	biopolymers					204:214	natural biopolymers	196:214	natural biopolymers	196:214	Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as controlled local drug delivery systems.
33957063	7	22	theme	only	1297:1300	arg1	CMCH-Ms					1302:1308	only CMCH-Ms	1297:1308	only CMCH-Ms	1297:1308	Importantly, less burst drug release and long-term sustained-release from the CMCH-Ms/HPCH composite hydrogel was observed than those from only CMCH-Ms or HPCH hydrogel.
33957063	4	23	theme	drug	844:847	arg1	loading					849:855	drug loading	844:855	drug loading	844:855	The morphology, gelation rate, swelling, rheological and mechanical properties, in vitro degradation and cytotoxicity, drug loading and drug release of the CMCH-Ms/HPCH gel scaffolds were examined.
33957063	8	24	theme	great	1380:1384	arg1	application					1396:1406	great potential application	1380:1406	great potential application for loading different drugs and sustained drug release in controlled local drug delivery systems	1380:1503	Thus, the composite CMCH-Ms/HPCH hydrogel exhibited great potential application for loading different drugs and sustained drug release in controlled local drug delivery systems.
33957063	2	25	theme	organic	495:501	arg1	solvents					503:510	organic solvents	495:510	organic solvents	495:510	In this study, we prepared carboxymethyl chitin microspheres (CMCH-Ms) with a diameter of 10-100 μm through physical crosslinking by increasing temperature in an aqueous two-phase system without using organic solvents, surfactants and crosslinking agents.
33957063	1	26	attach	derived	183:189	arg2	hydrogels					173:181	injectable hydrogels	162:181	injectable hydrogels	162:181	Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as controlled local drug delivery systems.
33957063	1	26	attach	derived	183:189	arg2	systems					285:291	controlled local drug delivery systems	254:291	controlled local drug delivery systems	254:291	Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as controlled local drug delivery systems.
33957063	1	26	attach	derived	183:189	arg1	biopolymers					204:214	natural biopolymers	196:214	natural biopolymers	196:214	Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as controlled local drug delivery systems.
33957063	1	26	attach	derived	183:189	arg2	Microspheres					145:156	Microspheres	145:156	Microspheres	145:156	Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as controlled local drug delivery systems.
33957063	3	27	theme	pH	638:639	arg1	environments					641:652	different pH environments	628:652	different pH environments	628:652	The stable microspheres keeping spherical shape with porous microstructure in different pH environments were embeded in thermosensitive hydroxypropyl chitin (HPCH) hydrogels.
33957063	8	28	theme	CMCH-Ms/HPCH	1348:1359	arg1	hydrogel					1361:1368	the composite CMCH-Ms/HPCH hydrogel	1334:1368	the composite CMCH-Ms/HPCH hydrogel	1334:1368	Thus, the composite CMCH-Ms/HPCH hydrogel exhibited great potential application for loading different drugs and sustained drug release in controlled local drug delivery systems.
33957063	0	29	theme	Injectable	0:9	arg1	chitin					25:30	Injectable hydroxypropyl chitin	0:30	Injectable hydroxypropyl chitin	0:30	Injectable hydroxypropyl chitin hydrogels embedded with carboxymethyl chitin microspheres prepared via a solvent-free process for drug delivery.
33957063	7	30	theme	burst	1176:1180	arg1	release					1187:1193	burst drug release	1176:1193	burst drug release	1176:1193	Importantly, less burst drug release and long-term sustained-release from the CMCH-Ms/HPCH composite hydrogel was observed than those from only CMCH-Ms or HPCH hydrogel.
33957063	8	31	theme	drug	1483:1486	arg1	systems					1497:1503	controlled local drug delivery systems	1466:1503	controlled local drug delivery systems	1466:1503	Thus, the composite CMCH-Ms/HPCH hydrogel exhibited great potential application for loading different drugs and sustained drug release in controlled local drug delivery systems.
33957063	4	32	theme	gelation	741:748	arg1	rate					750:753	gelation rate	741:753	gelation rate	741:753	The morphology, gelation rate, swelling, rheological and mechanical properties, in vitro degradation and cytotoxicity, drug loading and drug release of the CMCH-Ms/HPCH gel scaffolds were examined.
33957063	6	33	theme	organic	1059:1065	arg1	solvent					1067:1073	no organic solvent	1056:1073	no organic solvent	1056:1073	Moreover, no organic solvent was used in the preparation and drug loading process of CMCH-Ms/HPCH gel scaffold.
33957063	8	34	theme	sustained	1440:1448	arg1	release					1455:1461	sustained drug release	1440:1461	sustained drug release	1440:1461	Thus, the composite CMCH-Ms/HPCH hydrogel exhibited great potential application for loading different drugs and sustained drug release in controlled local drug delivery systems.
33957063	2	35	theme	two-phase	464:472	arg1	system					474:479	an aqueous two-phase system	453:479	an aqueous two-phase system	453:479	In this study, we prepared carboxymethyl chitin microspheres (CMCH-Ms) with a diameter of 10-100 μm through physical crosslinking by increasing temperature in an aqueous two-phase system without using organic solvents, surfactants and crosslinking agents.
33957063	8	36	theme	delivery	1488:1495	arg1	systems					1497:1503	controlled local drug delivery systems	1466:1503	controlled local drug delivery systems	1466:1503	Thus, the composite CMCH-Ms/HPCH hydrogel exhibited great potential application for loading different drugs and sustained drug release in controlled local drug delivery systems.
33957063	8	37	theme	local	1477:1481	arg1	systems					1497:1503	controlled local drug delivery systems	1466:1503	controlled local drug delivery systems	1466:1503	Thus, the composite CMCH-Ms/HPCH hydrogel exhibited great potential application for loading different drugs and sustained drug release in controlled local drug delivery systems.
33957063	2	38	theme	chitin	335:340	arg1	microspheres					342:353	carboxymethyl chitin microspheres	321:353	carboxymethyl chitin microspheres (CMCH-Ms) with a diameter of 10-100 μm	321:392	In this study, we prepared carboxymethyl chitin microspheres (CMCH-Ms) with a diameter of 10-100 μm through physical crosslinking by increasing temperature in an aqueous two-phase system without using organic solvents, surfactants and crosslinking agents.
33957063	2	38	theme	chitin	335:340	arg1	CMCH-Ms					356:362	CMCH-Ms	356:362	CMCH-Ms	356:362	In this study, we prepared carboxymethyl chitin microspheres (CMCH-Ms) with a diameter of 10-100 μm through physical crosslinking by increasing temperature in an aqueous two-phase system without using organic solvents, surfactants and crosslinking agents.
33957063	2	39	theme	aqueous	456:462	arg1	system					474:479	an aqueous two-phase system	453:479	an aqueous two-phase system	453:479	In this study, we prepared carboxymethyl chitin microspheres (CMCH-Ms) with a diameter of 10-100 μm through physical crosslinking by increasing temperature in an aqueous two-phase system without using organic solvents, surfactants and crosslinking agents.
33957063	7	40	theme	composite	1249:1257	arg1	hydrogel					1259:1266	the CMCH-Ms/HPCH composite hydrogel	1232:1266	the CMCH-Ms/HPCH composite hydrogel	1232:1266	Importantly, less burst drug release and long-term sustained-release from the CMCH-Ms/HPCH composite hydrogel was observed than those from only CMCH-Ms or HPCH hydrogel.
33957063	4	41	theme	CMCH-Ms/HPCH	881:892	arg1	scaffolds					898:906	the CMCH-Ms/HPCH gel scaffolds	877:906	the CMCH-Ms/HPCH gel scaffolds	877:906	The morphology, gelation rate, swelling, rheological and mechanical properties, in vitro degradation and cytotoxicity, drug loading and drug release of the CMCH-Ms/HPCH gel scaffolds were examined.
33957063	5	42	theme	cytotoxicity	948:959	arg1	test					961:964	cytotoxicity test	948:964	cytotoxicity test	948:964	In vitro degradation and cytotoxicity test indicated that CMCH-Ms/HPCH gel scaffolds were biodegradable and non-cytotoxic.
33957063	8	43	theme	potential	1386:1394	arg1	application					1396:1406	great potential application	1380:1406	great potential application for loading different drugs and sustained drug release in controlled local drug delivery systems	1380:1503	Thus, the composite CMCH-Ms/HPCH hydrogel exhibited great potential application for loading different drugs and sustained drug release in controlled local drug delivery systems.
33957063	6	44	theme	loading	1112:1118	arg1	process					1120:1126	the preparation and drug loading process	1087:1126	the preparation and drug loading process of CMCH-Ms/HPCH gel scaffold	1087:1155	Moreover, no organic solvent was used in the preparation and drug loading process of CMCH-Ms/HPCH gel scaffold.
33957063	3	45	theme	thermosensitive	670:684	arg1	hydrogels					714:722	thermosensitive hydroxypropyl chitin (HPCH) hydrogels	670:722	thermosensitive hydroxypropyl chitin (HPCH) hydrogels	670:722	The stable microspheres keeping spherical shape with porous microstructure in different pH environments were embeded in thermosensitive hydroxypropyl chitin (HPCH) hydrogels.
33957063	5	46	theme	In	923:924	arg1	degradation					932:942	In vitro degradation	923:942	In vitro degradation	923:942	In vitro degradation and cytotoxicity test indicated that CMCH-Ms/HPCH gel scaffolds were biodegradable and non-cytotoxic.
33957063	1	47	theme	controlled	254:263	arg1	Microspheres					145:156	Microspheres	145:156	Microspheres	145:156	Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as controlled local drug delivery systems.
33957063	1	47	theme	controlled	254:263	arg1	hydrogels					173:181	injectable hydrogels	162:181	injectable hydrogels	162:181	Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as controlled local drug delivery systems.
33957063	1	47	theme	controlled	254:263	arg1	systems					285:291	controlled local drug delivery systems	254:291	controlled local drug delivery systems	254:291	Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as controlled local drug delivery systems.
33957063	0	48	theme	chitin	70:75	arg1	microspheres					77:88	carboxymethyl chitin microspheres	56:88	carboxymethyl chitin microspheres prepared via a solvent-free process for drug delivery	56:142	Injectable hydroxypropyl chitin hydrogels embedded with carboxymethyl chitin microspheres prepared via a solvent-free process for drug delivery.
33957063	4	49	theme	scaffolds	898:906	arg1	release					866:872	drug release	861:872	drug release	861:872	The morphology, gelation rate, swelling, rheological and mechanical properties, in vitro degradation and cytotoxicity, drug loading and drug release of the CMCH-Ms/HPCH gel scaffolds were examined.
33957063	4	49	theme	scaffolds	898:906	arg1	properties					793:802	rheological and mechanical properties	766:802	properties	793:802	The morphology, gelation rate, swelling, rheological and mechanical properties, in vitro degradation and cytotoxicity, drug loading and drug release of the CMCH-Ms/HPCH gel scaffolds were examined.
33957063	4	49	theme	scaffolds	898:906	arg1	cytotoxicity					830:841	cytotoxicity	830:841	cytotoxicity	830:841	The morphology, gelation rate, swelling, rheological and mechanical properties, in vitro degradation and cytotoxicity, drug loading and drug release of the CMCH-Ms/HPCH gel scaffolds were examined.
33957063	4	49	theme	scaffolds	898:906	arg1	swelling					756:763	swelling	756:763	swelling	756:763	The morphology, gelation rate, swelling, rheological and mechanical properties, in vitro degradation and cytotoxicity, drug loading and drug release of the CMCH-Ms/HPCH gel scaffolds were examined.
33957063	4	49	theme	scaffolds	898:906	arg1	loading					849:855	drug loading	844:855	drug loading	844:855	The morphology, gelation rate, swelling, rheological and mechanical properties, in vitro degradation and cytotoxicity, drug loading and drug release of the CMCH-Ms/HPCH gel scaffolds were examined.
33957063	4	49	theme	scaffolds	898:906	arg1	morphology					729:738	morphology	729:738	morphology	729:738	The morphology, gelation rate, swelling, rheological and mechanical properties, in vitro degradation and cytotoxicity, drug loading and drug release of the CMCH-Ms/HPCH gel scaffolds were examined.
33957063	4	49	theme	scaffolds	898:906	arg1	degradation					814:824	degradation	814:824	degradation	814:824	The morphology, gelation rate, swelling, rheological and mechanical properties, in vitro degradation and cytotoxicity, drug loading and drug release of the CMCH-Ms/HPCH gel scaffolds were examined.
33957063	4	49	theme	scaffolds	898:906	arg1	rate					750:753	gelation rate	741:753	gelation rate	741:753	The morphology, gelation rate, swelling, rheological and mechanical properties, in vitro degradation and cytotoxicity, drug loading and drug release of the CMCH-Ms/HPCH gel scaffolds were examined.
33957063	4	50	theme	mechanical	782:791	arg1	properties					793:802	rheological and mechanical properties	766:802	properties	793:802	The morphology, gelation rate, swelling, rheological and mechanical properties, in vitro degradation and cytotoxicity, drug loading and drug release of the CMCH-Ms/HPCH gel scaffolds were examined.
33957063	0	51	theme	carboxymethyl	56:68	arg1	microspheres					77:88	carboxymethyl chitin microspheres	56:88	carboxymethyl chitin microspheres prepared via a solvent-free process for drug delivery	56:142	Injectable hydroxypropyl chitin hydrogels embedded with carboxymethyl chitin microspheres prepared via a solvent-free process for drug delivery.
33957063	6	52	theme	scaffold	1148:1155	arg1	process					1120:1126	the preparation and drug loading process	1087:1126	the preparation and drug loading process of CMCH-Ms/HPCH gel scaffold	1087:1155	Moreover, no organic solvent was used in the preparation and drug loading process of CMCH-Ms/HPCH gel scaffold.
33957063	4	53	theme	gel	894:896	arg1	scaffolds					898:906	the CMCH-Ms/HPCH gel scaffolds	877:906	the CMCH-Ms/HPCH gel scaffolds	877:906	The morphology, gelation rate, swelling, rheological and mechanical properties, in vitro degradation and cytotoxicity, drug loading and drug release of the CMCH-Ms/HPCH gel scaffolds were examined.
33957063	4	54	dep	morphology	729:738	arg1	The					725:727	The	725:727	The	725:727	The morphology, gelation rate, swelling, rheological and mechanical properties, in vitro degradation and cytotoxicity, drug loading and drug release of the CMCH-Ms/HPCH gel scaffolds were examined.
33957063	7	55	theme	CMCH-Ms/HPCH	1236:1247	arg1	hydrogel					1259:1266	the CMCH-Ms/HPCH composite hydrogel	1232:1266	the CMCH-Ms/HPCH composite hydrogel	1232:1266	Importantly, less burst drug release and long-term sustained-release from the CMCH-Ms/HPCH composite hydrogel was observed than those from only CMCH-Ms or HPCH hydrogel.
33957063	1	56	theme	injectable	162:171	arg1	Microspheres					145:156	Microspheres	145:156	Microspheres	145:156	Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as controlled local drug delivery systems.
33957063	1	56	theme	injectable	162:171	arg1	hydrogels					173:181	injectable hydrogels	162:181	injectable hydrogels	162:181	Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as controlled local drug delivery systems.
33957063	1	56	theme	injectable	162:171	arg1	systems					285:291	controlled local drug delivery systems	254:291	controlled local drug delivery systems	254:291	Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as controlled local drug delivery systems.
33957063	4	57	theme	drug	861:864	arg1	release					866:872	drug release	861:872	drug release	861:872	The morphology, gelation rate, swelling, rheological and mechanical properties, in vitro degradation and cytotoxicity, drug loading and drug release of the CMCH-Ms/HPCH gel scaffolds were examined.
33957063	6	58	theme	drug	1107:1110	arg1	process					1120:1126	the preparation and drug loading process	1087:1126	the preparation and drug loading process of CMCH-Ms/HPCH gel scaffold	1087:1155	Moreover, no organic solvent was used in the preparation and drug loading process of CMCH-Ms/HPCH gel scaffold.
33957063	8	59	theme	different	1420:1428	arg1	drugs					1430:1434	different drugs	1420:1434	different drugs	1420:1434	Thus, the composite CMCH-Ms/HPCH hydrogel exhibited great potential application for loading different drugs and sustained drug release in controlled local drug delivery systems.
33957063	1	60	theme	local	265:269	arg1	Microspheres					145:156	Microspheres	145:156	Microspheres	145:156	Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as controlled local drug delivery systems.
33957063	1	60	theme	local	265:269	arg1	hydrogels					173:181	injectable hydrogels	162:181	injectable hydrogels	162:181	Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as controlled local drug delivery systems.
33957063	1	60	theme	local	265:269	arg1	systems					285:291	controlled local drug delivery systems	254:291	controlled local drug delivery systems	254:291	Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as controlled local drug delivery systems.
33957063	8	61	theme	controlled	1466:1475	arg1	systems					1497:1503	controlled local drug delivery systems	1466:1503	controlled local drug delivery systems	1466:1503	Thus, the composite CMCH-Ms/HPCH hydrogel exhibited great potential application for loading different drugs and sustained drug release in controlled local drug delivery systems.
33957063	8	62	dep	loading	1412:1418	arg1	drugs					1430:1434	different drugs	1420:1434	different drugs	1420:1434	Thus, the composite CMCH-Ms/HPCH hydrogel exhibited great potential application for loading different drugs and sustained drug release in controlled local drug delivery systems.
33957063	3	63	theme	HPCH	708:711	arg1	hydrogels					714:722	thermosensitive hydroxypropyl chitin (HPCH) hydrogels	670:722	thermosensitive hydroxypropyl chitin (HPCH) hydrogels	670:722	The stable microspheres keeping spherical shape with porous microstructure in different pH environments were embeded in thermosensitive hydroxypropyl chitin (HPCH) hydrogels.
33957063	3	64	theme	spherical	582:590	arg1	shape					592:596	spherical shape	582:596	spherical shape	582:596	The stable microspheres keeping spherical shape with porous microstructure in different pH environments were embeded in thermosensitive hydroxypropyl chitin (HPCH) hydrogels.
33957063	8	65	theme	drug	1450:1453	arg1	release					1455:1461	sustained drug release	1440:1461	sustained drug release	1440:1461	Thus, the composite CMCH-Ms/HPCH hydrogel exhibited great potential application for loading different drugs and sustained drug release in controlled local drug delivery systems.
33957063	1	66	theme	drug	271:274	arg1	Microspheres					145:156	Microspheres	145:156	Microspheres	145:156	Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as controlled local drug delivery systems.
33957063	1	66	theme	drug	271:274	arg1	hydrogels					173:181	injectable hydrogels	162:181	injectable hydrogels	162:181	Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as controlled local drug delivery systems.
33957063	1	66	theme	drug	271:274	arg1	systems					285:291	controlled local drug delivery systems	254:291	controlled local drug delivery systems	254:291	Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as controlled local drug delivery systems.
33957063	3	67	from	microstructure	610:623	arg1	environments					641:652	different pH environments	628:652	different pH environments	628:652	The stable microspheres keeping spherical shape with porous microstructure in different pH environments were embeded in thermosensitive hydroxypropyl chitin (HPCH) hydrogels.
33957063	3	68	theme	hydroxypropyl	686:698	arg1	hydrogels					714:722	thermosensitive hydroxypropyl chitin (HPCH) hydrogels	670:722	thermosensitive hydroxypropyl chitin (HPCH) hydrogels	670:722	The stable microspheres keeping spherical shape with porous microstructure in different pH environments were embeded in thermosensitive hydroxypropyl chitin (HPCH) hydrogels.
33957063	6	69	theme	gel	1144:1146	arg1	scaffold					1148:1155	CMCH-Ms/HPCH gel scaffold	1131:1155	CMCH-Ms/HPCH gel scaffold	1131:1155	Moreover, no organic solvent was used in the preparation and drug loading process of CMCH-Ms/HPCH gel scaffold.
33957063	2	70	theme	physical	402:409	arg1	crosslinking					411:422	physical crosslinking	402:422	physical crosslinking	402:422	In this study, we prepared carboxymethyl chitin microspheres (CMCH-Ms) with a diameter of 10-100 μm through physical crosslinking by increasing temperature in an aqueous two-phase system without using organic solvents, surfactants and crosslinking agents.
33957063	8	71	from	release	1455:1461	arg1	systems					1497:1503	controlled local drug delivery systems	1466:1503	controlled local drug delivery systems	1466:1503	Thus, the composite CMCH-Ms/HPCH hydrogel exhibited great potential application for loading different drugs and sustained drug release in controlled local drug delivery systems.
33957063	1	72	theme	delivery	276:283	arg1	Microspheres					145:156	Microspheres	145:156	Microspheres	145:156	Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as controlled local drug delivery systems.
33957063	1	72	theme	delivery	276:283	arg1	hydrogels					173:181	injectable hydrogels	162:181	injectable hydrogels	162:181	Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as controlled local drug delivery systems.
33957063	1	72	theme	delivery	276:283	arg1	systems					285:291	controlled local drug delivery systems	254:291	controlled local drug delivery systems	254:291	Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as controlled local drug delivery systems.
33957063	3	73	theme	porous	603:608	arg1	microstructure					610:623	porous microstructure	603:623	porous microstructure in different pH environments	603:652	The stable microspheres keeping spherical shape with porous microstructure in different pH environments were embeded in thermosensitive hydroxypropyl chitin (HPCH) hydrogels.
33957063	3	74	theme	chitin	700:705	arg1	hydrogels					714:722	thermosensitive hydroxypropyl chitin (HPCH) hydrogels	670:722	thermosensitive hydroxypropyl chitin (HPCH) hydrogels	670:722	The stable microspheres keeping spherical shape with porous microstructure in different pH environments were embeded in thermosensitive hydroxypropyl chitin (HPCH) hydrogels.
33957063	0	75	theme	hydroxypropyl	11:23	arg1	chitin					25:30	Injectable hydroxypropyl chitin	0:30	Injectable hydroxypropyl chitin	0:30	Injectable hydroxypropyl chitin hydrogels embedded with carboxymethyl chitin microspheres prepared via a solvent-free process for drug delivery.
33957063	6	76	theme	preparation	1091:1101	arg1	process					1120:1126	the preparation and drug loading process	1087:1126	the preparation and drug loading process of CMCH-Ms/HPCH gel scaffold	1087:1155	Moreover, no organic solvent was used in the preparation and drug loading process of CMCH-Ms/HPCH gel scaffold.
33328579	3	0	theme	processable	744:754	arg1	scaffold					767:774	a conductive, hydrophilic, processable and stable scaffold	717:774	a conductive, hydrophilic, processable and stable scaffold	717:774	In addition, chitosan-based nanoparticles were synthesized by ionic gelation method and after characterization merged into PPy-Alg composite in order to fabricate a conductive, hydrophilic, processable and stable scaffold.
33328579	2	1	theme	different	369:377	arg1	percentages					379:389	different percentages	369:389	different percentages of alginate	369:401	In this study, polypyrrole (PPy)-alginate (Alg) composites were prepared using different percentages of alginate and pyrrole by oxidative polymerization method using FeCl3 as an oxidant and electrical conductivity of composites were measured by four probe method.
33328579	2	2	theme	composites	507:516	arg1	FeCl3					456:460	FeCl3	456:460	FeCl3	456:460	In this study, polypyrrole (PPy)-alginate (Alg) composites were prepared using different percentages of alginate and pyrrole by oxidative polymerization method using FeCl3 as an oxidant and electrical conductivity of composites were measured by four probe method.
33328579	2	2	theme	composites	507:516	arg1	conductivity					491:502	an oxidant and electrical conductivity	465:502	an oxidant and electrical conductivity of composites	465:516	In this study, polypyrrole (PPy)-alginate (Alg) composites were prepared using different percentages of alginate and pyrrole by oxidative polymerization method using FeCl3 as an oxidant and electrical conductivity of composites were measured by four probe method.
33328579	7	3	from	FT-IR	1229:1233	arg1	Results					1216:1222	Results	1216:1222	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells	1216:1369	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	4	theme	Nanochitosan/PPy-Alg	1405:1424	arg1	scaffold					1426:1433	the Nanochitosan/PPy-Alg scaffold	1401:1433	the Nanochitosan/PPy-Alg scaffold	1401:1433	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	6	5	theme	high	1094:1097	arg1	conductivity					1099:1110	the high conductivity	1090:1110	the high conductivity	1090:1110	Due to the high conductivity, the film with ratio 2:10 (PPy-Alg) was recognized more suitable for fabrication of the final scaffold.
33328579	3	6	theme	hydrophilic	731:741	arg1	scaffold					767:774	a conductive, hydrophilic, processable and stable scaffold	717:774	a conductive, hydrophilic, processable and stable scaffold	717:774	In addition, chitosan-based nanoparticles were synthesized by ionic gelation method and after characterization merged into PPy-Alg composite in order to fabricate a conductive, hydrophilic, processable and stable scaffold.
33328579	7	7	from	viability	1305:1313	arg1	Results					1216:1222	Results	1216:1222	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells	1216:1369	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	2	8	theme	PPy	318:320	arg1	-alginate					322:330	polypyrrole (PPy)-alginate	305:330	polypyrrole (PPy)-alginate (Alg) composites	305:347	In this study, polypyrrole (PPy)-alginate (Alg) composites were prepared using different percentages of alginate and pyrrole by oxidative polymerization method using FeCl3 as an oxidant and electrical conductivity of composites were measured by four probe method.
33328579	2	8	theme	PPy	318:320	arg1	Alg					333:335	Alg	333:335	Alg	333:335	In this study, polypyrrole (PPy)-alginate (Alg) composites were prepared using different percentages of alginate and pyrrole by oxidative polymerization method using FeCl3 as an oxidant and electrical conductivity of composites were measured by four probe method.
33328579	7	9	from	SEM	1239:1241	arg1	Results					1216:1222	Results	1216:1222	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells	1216:1369	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	4	10	theme	scaffold	833:840	arg1	characterization					792:807	Physiochemical characterization	777:807	Physiochemical characterization of nanochitosan/PPy-Alg scaffold such as electrical conductivity, porosity, swelling and degradation	777:908	Physiochemical characterization of nanochitosan/PPy-Alg scaffold such as electrical conductivity, porosity, swelling and degradation was investigated.
33328579	3	11	theme	chitosan-based	567:580	arg1	nanoparticles					582:594	chitosan-based nanoparticles	567:594	chitosan-based nanoparticles	567:594	In addition, chitosan-based nanoparticles were synthesized by ionic gelation method and after characterization merged into PPy-Alg composite in order to fabricate a conductive, hydrophilic, processable and stable scaffold.
33328579	2	12	theme	probe	540:544	arg1	method					546:551	four probe method	535:551	four probe method	535:551	In this study, polypyrrole (PPy)-alginate (Alg) composites were prepared using different percentages of alginate and pyrrole by oxidative polymerization method using FeCl3 as an oxidant and electrical conductivity of composites were measured by four probe method.
33328579	5	13	theme	neural	1003:1008	arg1	cells					1039:1043	OLN-93 neural and human dermal fibroblasts cells	996:1043	OLN-93 neural and human dermal fibroblasts cells	996:1043	Moreover, cytotoxicity and proliferation were examined by culturing OLN-93 neural and human dermal fibroblasts cells on the Nanochitosan/PPy-Alg scaffold.
33328579	2	14	theme	polypyrrole	305:315	arg1	-alginate					322:330	polypyrrole (PPy)-alginate	305:330	polypyrrole (PPy)-alginate (Alg) composites	305:347	In this study, polypyrrole (PPy)-alginate (Alg) composites were prepared using different percentages of alginate and pyrrole by oxidative polymerization method using FeCl3 as an oxidant and electrical conductivity of composites were measured by four probe method.
33328579	2	14	theme	polypyrrole	305:315	arg1	Alg					333:335	Alg	333:335	Alg	333:335	In this study, polypyrrole (PPy)-alginate (Alg) composites were prepared using different percentages of alginate and pyrrole by oxidative polymerization method using FeCl3 as an oxidant and electrical conductivity of composites were measured by four probe method.
33328579	1	15	theme	much	203:206	arg1	interest					208:215	much interest	203:215	much interest	203:215	The utilization of conductive polymers for fabrication of neural scaffolds have attracted much interest because of providing a microenvironment which can imitate nerve tissues.
33328579	7	16	from	evaluation	1244:1253	arg1	Results					1216:1222	Results	1216:1222	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells	1216:1369	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	17	theme	scaffold	1426:1433	arg1	cytocompatiblity					1381:1396	cytocompatiblity	1381:1396	cytocompatiblity of the Nanochitosan/PPy-Alg scaffold	1381:1433	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	2	18	theme	oxidant	468:474	arg1	FeCl3					456:460	FeCl3	456:460	FeCl3	456:460	In this study, polypyrrole (PPy)-alginate (Alg) composites were prepared using different percentages of alginate and pyrrole by oxidative polymerization method using FeCl3 as an oxidant and electrical conductivity of composites were measured by four probe method.
33328579	2	18	theme	oxidant	468:474	arg1	conductivity					491:502	an oxidant and electrical conductivity	465:502	an oxidant and electrical conductivity of composites	465:516	In this study, polypyrrole (PPy)-alginate (Alg) composites were prepared using different percentages of alginate and pyrrole by oxidative polymerization method using FeCl3 as an oxidant and electrical conductivity of composites were measured by four probe method.
33328579	3	19	theme	stable	760:765	arg1	scaffold					767:774	a conductive, hydrophilic, processable and stable scaffold	717:774	a conductive, hydrophilic, processable and stable scaffold	717:774	In addition, chitosan-based nanoparticles were synthesized by ionic gelation method and after characterization merged into PPy-Alg composite in order to fabricate a conductive, hydrophilic, processable and stable scaffold.
33328579	5	20	theme	fibroblasts	1027:1037	arg1	cells					1039:1043	OLN-93 neural and human dermal fibroblasts cells	996:1043	OLN-93 neural and human dermal fibroblasts cells	996:1043	Moreover, cytotoxicity and proliferation were examined by culturing OLN-93 neural and human dermal fibroblasts cells on the Nanochitosan/PPy-Alg scaffold.
33328579	7	21	from	swelling	1268:1275	arg1	Results					1216:1222	Results	1216:1222	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells	1216:1369	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	4	22	theme	Physiochemical	777:790	arg1	characterization					792:807	Physiochemical characterization	777:807	Physiochemical characterization of nanochitosan/PPy-Alg scaffold such as electrical conductivity, porosity, swelling and degradation	777:908	Physiochemical characterization of nanochitosan/PPy-Alg scaffold such as electrical conductivity, porosity, swelling and degradation was investigated.
33328579	2	23	theme	alginate	394:401	arg1	percentages					379:389	different percentages	369:389	different percentages of alginate	369:401	In this study, polypyrrole (PPy)-alginate (Alg) composites were prepared using different percentages of alginate and pyrrole by oxidative polymerization method using FeCl3 as an oxidant and electrical conductivity of composites were measured by four probe method.
33328579	8	24	theme	tissue	1554:1559	arg1	engineering					1561:1571	neural tissue engineering	1547:1571	neural tissue engineering	1547:1571	Based on the features of the constructed scaffold, Nanochitosan/PPy-Alg scaffold can be a proper candidate for neural tissue engineering.
33328579	6	25	with	film	1117:1120	arg1	PPy-Alg					1139:1145	PPy-Alg	1139:1145	PPy-Alg	1139:1145	Due to the high conductivity, the film with ratio 2:10 (PPy-Alg) was recognized more suitable for fabrication of the final scaffold.
33328579	6	25	with	film	1117:1120	arg1	2:10					1133:1136	ratio 2:10	1127:1136	ratio 2:10 (PPy-Alg)	1127:1146	Due to the high conductivity, the film with ratio 2:10 (PPy-Alg) was recognized more suitable for fabrication of the final scaffold.
33328579	2	26	theme	-alginate	322:330	arg1	composites					338:347	polypyrrole (PPy)-alginate (Alg) composites	305:347	polypyrrole (PPy)-alginate (Alg) composites	305:347	In this study, polypyrrole (PPy)-alginate (Alg) composites were prepared using different percentages of alginate and pyrrole by oxidative polymerization method using FeCl3 as an oxidant and electrical conductivity of composites were measured by four probe method.
33328579	0	27	theme	nanochitosan	15:26	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of nanochitosan	0:26	Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering.
33328579	4	28	theme	electrical	850:859	arg1	conductivity					861:872	electrical conductivity	850:872	electrical conductivity	850:872	Physiochemical characterization of nanochitosan/PPy-Alg scaffold such as electrical conductivity, porosity, swelling and degradation was investigated.
33328579	1	29	theme	conductive	132:141	arg1	polymers					143:150	conductive polymers	132:150	conductive polymers	132:150	The utilization of conductive polymers for fabrication of neural scaffolds have attracted much interest because of providing a microenvironment which can imitate nerve tissues.
33328579	0	30	theme	conducting	62:71	arg1	scaffold					73:80	polypyrrole/alginate conducting scaffold	41:80	polypyrrole/alginate conducting scaffold for neural tissue engineering	41:110	Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering.
33328579	7	31	theme	cells	1365:1369	arg1	swelling					1268:1275	swelling	1268:1275	swelling	1268:1275	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	31	theme	cells	1365:1369	arg1	viability					1305:1313	viability	1305:1313	viability	1305:1313	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	31	theme	cells	1365:1369	arg1	SEM					1239:1241	SEM	1239:1241	SEM	1239:1241	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	31	theme	cells	1365:1369	arg1	proliferation					1319:1331	proliferation	1319:1331	proliferation	1319:1331	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	31	theme	cells	1365:1369	arg1	evaluation					1244:1253	evaluation	1244:1253	evaluation of porosity	1244:1265	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	31	theme	cells	1365:1369	arg1	degradation					1281:1291	degradation	1281:1291	degradation	1281:1291	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	31	theme	cells	1365:1369	arg1	FT-IR					1229:1233	FT-IR	1229:1233	FT-IR	1229:1233	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	32	theme	fibroblast	1354:1363	arg1	cells					1365:1369	fibroblast cells	1354:1369	fibroblast cells	1354:1369	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	2	33	theme	polymerization	428:441	arg1	method					443:448	oxidative polymerization method	418:448	oxidative polymerization method using FeCl3 as an oxidant and electrical conductivity of composites	418:516	In this study, polypyrrole (PPy)-alginate (Alg) composites were prepared using different percentages of alginate and pyrrole by oxidative polymerization method using FeCl3 as an oxidant and electrical conductivity of composites were measured by four probe method.
33328579	0	34	theme	polypyrrole/alginate	41:60	arg1	scaffold					73:80	polypyrrole/alginate conducting scaffold	41:80	polypyrrole/alginate conducting scaffold for neural tissue engineering	41:110	Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering.
33328579	3	35	theme	PPy-Alg	677:683	arg1	composite					685:693	PPy-Alg composite	677:693	PPy-Alg composite	677:693	In addition, chitosan-based nanoparticles were synthesized by ionic gelation method and after characterization merged into PPy-Alg composite in order to fabricate a conductive, hydrophilic, processable and stable scaffold.
33328579	8	36	theme	scaffold	1477:1484	arg1	features					1449:1456	the features	1445:1456	the features of the constructed scaffold	1445:1484	Based on the features of the constructed scaffold, Nanochitosan/PPy-Alg scaffold can be a proper candidate for neural tissue engineering.
33328579	2	37	theme	oxidative	418:426	arg1	method					443:448	oxidative polymerization method	418:448	oxidative polymerization method using FeCl3 as an oxidant and electrical conductivity of composites	418:516	In this study, polypyrrole (PPy)-alginate (Alg) composites were prepared using different percentages of alginate and pyrrole by oxidative polymerization method using FeCl3 as an oxidant and electrical conductivity of composites were measured by four probe method.
33328579	2	38	theme	electrical	480:489	arg1	FeCl3					456:460	FeCl3	456:460	FeCl3	456:460	In this study, polypyrrole (PPy)-alginate (Alg) composites were prepared using different percentages of alginate and pyrrole by oxidative polymerization method using FeCl3 as an oxidant and electrical conductivity of composites were measured by four probe method.
33328579	2	38	theme	electrical	480:489	arg1	conductivity					491:502	an oxidant and electrical conductivity	465:502	an oxidant and electrical conductivity of composites	465:516	In this study, polypyrrole (PPy)-alginate (Alg) composites were prepared using different percentages of alginate and pyrrole by oxidative polymerization method using FeCl3 as an oxidant and electrical conductivity of composites were measured by four probe method.
33328579	8	39	theme	neural	1547:1552	arg1	engineering					1561:1571	neural tissue engineering	1547:1571	neural tissue engineering	1547:1571	Based on the features of the constructed scaffold, Nanochitosan/PPy-Alg scaffold can be a proper candidate for neural tissue engineering.
33328579	3	40	theme	ionic	616:620	arg1	method					631:636	ionic gelation method	616:636	ionic gelation method	616:636	In addition, chitosan-based nanoparticles were synthesized by ionic gelation method and after characterization merged into PPy-Alg composite in order to fabricate a conductive, hydrophilic, processable and stable scaffold.
33328579	1	41	theme	polymers	143:150	arg1	utilization					117:127	The utilization	113:127	The utilization of conductive polymers for fabrication of neural scaffolds	113:186	The utilization of conductive polymers for fabrication of neural scaffolds have attracted much interest because of providing a microenvironment which can imitate nerve tissues.
33328579	7	42	from	degradation	1281:1291	arg1	Results					1216:1222	Results	1216:1222	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells	1216:1369	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	3	43	theme	gelation	622:629	arg1	method					631:636	ionic gelation method	616:636	ionic gelation method	616:636	In addition, chitosan-based nanoparticles were synthesized by ionic gelation method and after characterization merged into PPy-Alg composite in order to fabricate a conductive, hydrophilic, processable and stable scaffold.
33328579	7	44	theme	neural	1343:1348	arg1	swelling					1268:1275	swelling	1268:1275	swelling	1268:1275	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	44	theme	neural	1343:1348	arg1	viability					1305:1313	viability	1305:1313	viability	1305:1313	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	44	theme	neural	1343:1348	arg1	SEM					1239:1241	SEM	1239:1241	SEM	1239:1241	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	44	theme	neural	1343:1348	arg1	proliferation					1319:1331	proliferation	1319:1331	proliferation	1319:1331	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	44	theme	neural	1343:1348	arg1	evaluation					1244:1253	evaluation	1244:1253	evaluation of porosity	1244:1265	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	44	theme	neural	1343:1348	arg1	degradation					1281:1291	degradation	1281:1291	degradation	1281:1291	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	44	theme	neural	1343:1348	arg1	FT-IR					1229:1233	FT-IR	1229:1233	FT-IR	1229:1233	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	6	45	theme	scaffold	1206:1213	arg1	fabrication					1181:1191	fabrication	1181:1191	fabrication of the final scaffold	1181:1213	Due to the high conductivity, the film with ratio 2:10 (PPy-Alg) was recognized more suitable for fabrication of the final scaffold.
33328579	3	46	theme	conductive	719:728	arg1	scaffold					767:774	a conductive, hydrophilic, processable and stable scaffold	717:774	a conductive, hydrophilic, processable and stable scaffold	717:774	In addition, chitosan-based nanoparticles were synthesized by ionic gelation method and after characterization merged into PPy-Alg composite in order to fabricate a conductive, hydrophilic, processable and stable scaffold.
33328579	5	47	theme	human	1014:1018	arg1	fibroblasts					1027:1037	human dermal fibroblasts	1014:1037	human dermal fibroblasts	1014:1037	Moreover, cytotoxicity and proliferation were examined by culturing OLN-93 neural and human dermal fibroblasts cells on the Nanochitosan/PPy-Alg scaffold.
33328579	4	48	theme	nanochitosan/PPy-Alg	812:831	arg1	scaffold					833:840	nanochitosan/PPy-Alg scaffold	812:840	nanochitosan/PPy-Alg scaffold such as electrical conductivity, porosity, swelling and degradation	812:908	Physiochemical characterization of nanochitosan/PPy-Alg scaffold such as electrical conductivity, porosity, swelling and degradation was investigated.
33328579	5	49	theme	Nanochitosan/PPy-Alg	1052:1071	arg1	scaffold					1073:1080	the Nanochitosan/PPy-Alg scaffold	1048:1080	the Nanochitosan/PPy-Alg scaffold	1048:1080	Moreover, cytotoxicity and proliferation were examined by culturing OLN-93 neural and human dermal fibroblasts cells on the Nanochitosan/PPy-Alg scaffold.
33328579	5	50	theme	dermal	1020:1025	arg1	fibroblasts					1027:1037	human dermal fibroblasts	1014:1037	human dermal fibroblasts	1014:1037	Moreover, cytotoxicity and proliferation were examined by culturing OLN-93 neural and human dermal fibroblasts cells on the Nanochitosan/PPy-Alg scaffold.
33328579	6	51	theme	final	1200:1204	arg1	scaffold					1206:1213	the final scaffold	1196:1213	the final scaffold	1196:1213	Due to the high conductivity, the film with ratio 2:10 (PPy-Alg) was recognized more suitable for fabrication of the final scaffold.
33328579	0	52	theme	tissue	93:98	arg1	engineering					100:110	neural tissue engineering	86:110	neural tissue engineering	86:110	Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering.
33328579	6	53	theme	ratio	1127:1131	arg1	PPy-Alg					1139:1145	PPy-Alg	1139:1145	PPy-Alg	1139:1145	Due to the high conductivity, the film with ratio 2:10 (PPy-Alg) was recognized more suitable for fabrication of the final scaffold.
33328579	6	53	theme	ratio	1127:1131	arg1	2:10					1133:1136	ratio 2:10	1127:1136	ratio 2:10 (PPy-Alg)	1127:1146	Due to the high conductivity, the film with ratio 2:10 (PPy-Alg) was recognized more suitable for fabrication of the final scaffold.
33328579	8	54	theme	proper	1526:1531	arg1	candidate					1533:1541	a proper candidate	1524:1541	a proper candidate for neural tissue engineering	1524:1571	Based on the features of the constructed scaffold, Nanochitosan/PPy-Alg scaffold can be a proper candidate for neural tissue engineering.
33328579	8	54	theme	proper	1526:1531	arg1	scaffold					1508:1515	Nanochitosan/PPy-Alg scaffold	1487:1515	Nanochitosan/PPy-Alg scaffold	1487:1515	Based on the features of the constructed scaffold, Nanochitosan/PPy-Alg scaffold can be a proper candidate for neural tissue engineering.
33328579	1	55	theme	neural	171:176	arg1	scaffolds					178:186	neural scaffolds	171:186	neural scaffolds	171:186	The utilization of conductive polymers for fabrication of neural scaffolds have attracted much interest because of providing a microenvironment which can imitate nerve tissues.
33328579	8	56	theme	constructed	1465:1475	arg1	scaffold					1477:1484	the constructed scaffold	1461:1484	the constructed scaffold	1461:1484	Based on the features of the constructed scaffold, Nanochitosan/PPy-Alg scaffold can be a proper candidate for neural tissue engineering.
33328579	1	57	theme	nerve	275:279	arg1	tissues					281:287	nerve tissues	275:287	nerve tissues	275:287	The utilization of conductive polymers for fabrication of neural scaffolds have attracted much interest because of providing a microenvironment which can imitate nerve tissues.
33328579	0	58	theme	neural	86:91	arg1	engineering					100:110	neural tissue engineering	86:110	neural tissue engineering	86:110	Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering.
33328579	7	59	theme	porosity	1258:1265	arg1	swelling					1268:1275	swelling	1268:1275	swelling	1268:1275	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	59	theme	porosity	1258:1265	arg1	viability					1305:1313	viability	1305:1313	viability	1305:1313	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	59	theme	porosity	1258:1265	arg1	SEM					1239:1241	SEM	1239:1241	SEM	1239:1241	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	59	theme	porosity	1258:1265	arg1	proliferation					1319:1331	proliferation	1319:1331	proliferation	1319:1331	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	59	theme	porosity	1258:1265	arg1	evaluation					1244:1253	evaluation	1244:1253	evaluation of porosity	1244:1265	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	59	theme	porosity	1258:1265	arg1	degradation					1281:1291	degradation	1281:1291	degradation	1281:1291	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	7	59	theme	porosity	1258:1265	arg1	FT-IR					1229:1233	FT-IR	1229:1233	FT-IR	1229:1233	Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy-Alg scaffold.
33328579	1	60	theme	scaffolds	178:186	arg1	fabrication					156:166	fabrication	156:166	fabrication of neural scaffolds	156:186	The utilization of conductive polymers for fabrication of neural scaffolds have attracted much interest because of providing a microenvironment which can imitate nerve tissues.
33328579	8	61	theme	Nanochitosan/PPy-Alg	1487:1506	arg1	candidate					1533:1541	a proper candidate	1524:1541	a proper candidate for neural tissue engineering	1524:1571	Based on the features of the constructed scaffold, Nanochitosan/PPy-Alg scaffold can be a proper candidate for neural tissue engineering.
33328579	8	61	theme	Nanochitosan/PPy-Alg	1487:1506	arg1	scaffold					1508:1515	Nanochitosan/PPy-Alg scaffold	1487:1515	Nanochitosan/PPy-Alg scaffold	1487:1515	Based on the features of the constructed scaffold, Nanochitosan/PPy-Alg scaffold can be a proper candidate for neural tissue engineering.
33418222	5	0	theme	6.983	729:733	arg1	m2/g					735:738	6.983 m2/g	729:738	6.983 m2/g	729:738	The amount of surface area was computed to be 44.689 m2/g, 0.0392 m2/g, and 6.983 m2/g for GO, AM-SA, and AM-GO-SA nanocomposite hydrogel, respectively.
33418222	6	1	theme	isotherm	880:887	arg1	model					889:893	the Redlich-Peterson isotherm model	859:893	the Redlich-Peterson isotherm model	859:893	The results showed that the experimental data follow the Redlich-Peterson isotherm model.
33418222	11	2	theme	kinetic	1472:1478	arg1	model					1480:1484	a pseudo- second- order (PSO) kinetic model	1442:1484	a pseudo- second- order (PSO) kinetic model	1442:1484	The experimental data followed a pseudo- second- order (PSO) kinetic model.
33418222	0	3	theme	nanocomposite	82:94	arg1	hydrogel					96:103	acrylamide/graphene oxide bonded sodium alginate nanocomposite hydrogel	33:103	acrylamide/graphene oxide bonded sodium alginate nanocomposite hydrogel	33:103	Crystal violet dye sorption over acrylamide/graphene oxide bonded sodium alginate nanocomposite hydrogel.
33418222	6	4	theme	Redlich-Peterson	863:878	arg1	model					889:893	the Redlich-Peterson isotherm model	859:893	the Redlich-Peterson isotherm model	859:893	The results showed that the experimental data follow the Redlich-Peterson isotherm model.
33418222	10	5	theme	adsorption	1350:1359	arg1	process					1361:1367	the adsorption process	1346:1367	the adsorption process	1346:1367	Besides, thermodynamic results showed that the adsorption process is better proceeding at low temperatures.
33418222	1	6	theme	hydrogel	165:172	arg1	synthesis					110:118	The synthesis	106:118	The synthesis of acrylamide bonded sodium alginate (AM-SA) hydrogel and acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel	106:259	The synthesis of acrylamide bonded sodium alginate (AM-SA) hydrogel and acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel was successfully performed using the free radical method.
33418222	4	7	theme	C-O	589:591	arg1	groups					605:610	The C-O, CO, and CC groups	585:610	The C-O, CO, and CC groups	585:610	The C-O, CO, and CC groups were detected in the produced hydrogels.
33418222	7	8	theme	AM-GO-SA	1009:1016	arg1	62.07 mg/g					1041:1050	AM-SA hydrogel and AM-GO-SA nanocomposite hydrogel 62.07 mg/g	990:1050	62.07 mg/g	1041:1050	Also, the maximum adsorption capacity of monolayer for CV dye adsorption was determined using AM-SA hydrogel and AM-GO-SA nanocomposite hydrogel 62.07 mg/g and 100.30 mg/g, respectively.
33418222	10	9	theme	low	1393:1395	arg1	temperatures					1397:1408	low temperatures	1393:1408	low temperatures	1393:1408	Besides, thermodynamic results showed that the adsorption process is better proceeding at low temperatures.
33418222	10	10	theme	thermodynamic	1312:1324	arg1	results					1326:1332	thermodynamic results	1312:1332	thermodynamic results	1312:1332	Besides, thermodynamic results showed that the adsorption process is better proceeding at low temperatures.
33418222	7	11	theme	maximum	906:912	arg1	capacity					925:932	the maximum adsorption capacity	902:932	the maximum adsorption capacity of monolayer for CV dye adsorption	902:967	Also, the maximum adsorption capacity of monolayer for CV dye adsorption was determined using AM-SA hydrogel and AM-GO-SA nanocomposite hydrogel 62.07 mg/g and 100.30 mg/g, respectively.
33418222	4	12	theme	CO	594:595	arg1	groups					605:610	The C-O, CO, and CC groups	585:610	The C-O, CO, and CC groups	585:610	The C-O, CO, and CC groups were detected in the produced hydrogels.
33418222	5	13	theme	0.0392	712:717	arg1	m2/g					719:722	0.0392 m2/g	712:722	0.0392 m2/g	712:722	The amount of surface area was computed to be 44.689 m2/g, 0.0392 m2/g, and 6.983 m2/g for GO, AM-SA, and AM-GO-SA nanocomposite hydrogel, respectively.
33418222	9	14	theme	CV	1256:1257	arg1	exothermic					1275:1284	exothermic	1275:1284	exothermic	1275:1284	Thermodynamically, the CV elimination was exothermic and spontaneous.
33418222	9	14	theme	CV	1256:1257	arg1	elimination					1259:1269	the CV elimination	1252:1269	the CV elimination	1252:1269	Thermodynamically, the CV elimination was exothermic and spontaneous.
33418222	7	15	theme	adsorption	914:923	arg1	capacity					925:932	the maximum adsorption capacity	902:932	the maximum adsorption capacity of monolayer for CV dye adsorption	902:967	Also, the maximum adsorption capacity of monolayer for CV dye adsorption was determined using AM-SA hydrogel and AM-GO-SA nanocomposite hydrogel 62.07 mg/g and 100.30 mg/g, respectively.
33418222	3	16	theme	adsorption	430:439	arg1	experiments					449:459	The adsorption process experiments	426:459	The adsorption process experiments	426:459	The adsorption process experiments were performed discontinuously and the acquired data showed that the efficiency is more dependent on pH than other factors.
33418222	8	17	theme	CV	1167:1168	arg1	dye					1170:1172	CV dye	1167:1172	CV dye using both types of adsorbents	1167:1203	In addition, the parameters RL, n, and E showed that the processes of adsorption of CV dye using both types of adsorbents are physical and desirable.
33418222	6	18	theme	experimental	834:845	arg1	data					847:850	the experimental data	830:850	the experimental data	830:850	The results showed that the experimental data follow the Redlich-Peterson isotherm model.
33418222	3	19	theme	process	441:447	arg1	experiments					449:459	The adsorption process experiments	426:459	The adsorption process experiments	426:459	The adsorption process experiments were performed discontinuously and the acquired data showed that the efficiency is more dependent on pH than other factors.
33418222	2	20	from	adsorbents	379:388	arg1	removal					417:423	crystal violet (CV) dye removal	393:423	crystal violet (CV) dye removal	393:423	The AM-SA and AM-GO-SA hydrogels were applied as composited adsorbents in crystal violet (CV) dye removal.
33418222	1	21	theme	acrylamide/graphene	178:196	arg1	hydrogel					252:259	acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel	178:259	acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel	178:259	The synthesis of acrylamide bonded sodium alginate (AM-SA) hydrogel and acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel was successfully performed using the free radical method.
33418222	0	22	theme	violet	8:13	arg1	dye					15:17	Crystal violet dye	0:17	Crystal violet dye	0:17	Crystal violet dye sorption over acrylamide/graphene oxide bonded sodium alginate nanocomposite hydrogel.
33418222	13	23	theme	aqueous	1751:1757	arg1	solution					1759:1766	aqueous solution	1751:1766	aqueous solution	1751:1766	Therefore, according to the obtained results, it can be stated that the produced hydrogels are efficient and viable composited adsorbent in removing CV dye from aqueous solution.
33418222	13	24	theme	CV	1739:1740	arg1	dye					1742:1744	CV dye	1739:1744	CV dye	1739:1744	Therefore, according to the obtained results, it can be stated that the produced hydrogels are efficient and viable composited adsorbent in removing CV dye from aqueous solution.
33418222	2	25	theme	AM-GO-SA	333:340	arg1	adsorbents					379:388	composited adsorbents	368:388	composited adsorbents in crystal violet (CV) dye removal	368:423	The AM-SA and AM-GO-SA hydrogels were applied as composited adsorbents in crystal violet (CV) dye removal.
33418222	2	25	theme	AM-GO-SA	333:340	arg1	hydrogels					342:350	The AM-SA and AM-GO-SA hydrogels	319:350	The AM-SA and AM-GO-SA hydrogels	319:350	The AM-SA and AM-GO-SA hydrogels were applied as composited adsorbents in crystal violet (CV) dye removal.
33418222	5	26	theme	surface	667:673	arg1	area					675:678	surface area	667:678	surface area	667:678	The amount of surface area was computed to be 44.689 m2/g, 0.0392 m2/g, and 6.983 m2/g for GO, AM-SA, and AM-GO-SA nanocomposite hydrogel, respectively.
33418222	1	27	theme	oxide	198:202	arg1	hydrogel					252:259	acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel	178:259	acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel	178:259	The synthesis of acrylamide bonded sodium alginate (AM-SA) hydrogel and acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel was successfully performed using the free radical method.
33418222	0	28	theme	Crystal	0:6	arg1	dye					15:17	Crystal violet dye	0:17	Crystal violet dye	0:17	Crystal violet dye sorption over acrylamide/graphene oxide bonded sodium alginate nanocomposite hydrogel.
33418222	1	29	theme	free	298:301	arg1	radical					303:309	the free radical	294:309	the free radical method	294:316	The synthesis of acrylamide bonded sodium alginate (AM-SA) hydrogel and acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel was successfully performed using the free radical method.
33418222	8	30	dep	parameters	1100:1109	arg1	parameters					1100:1109	the parameters	1096:1109	the parameters RL, n, and E	1096:1122	In addition, the parameters RL, n, and E showed that the processes of adsorption of CV dye using both types of adsorbents are physical and desirable.
33418222	8	30	dep	parameters	1100:1109	arg1	n					1115:1115	n	1115:1115	n	1115:1115	In addition, the parameters RL, n, and E showed that the processes of adsorption of CV dye using both types of adsorbents are physical and desirable.
33418222	8	30	dep	parameters	1100:1109	arg1	E					1122:1122	E	1122:1122	E	1122:1122	In addition, the parameters RL, n, and E showed that the processes of adsorption of CV dye using both types of adsorbents are physical and desirable.
33418222	8	30	dep	parameters	1100:1109	arg1	RL					1111:1112	RL	1111:1112	RL	1111:1112	In addition, the parameters RL, n, and E showed that the processes of adsorption of CV dye using both types of adsorbents are physical and desirable.
33418222	7	31	theme	hydrogel	1032:1039	arg1	62.07 mg/g					1041:1050	AM-SA hydrogel and AM-GO-SA nanocomposite hydrogel 62.07 mg/g	990:1050	62.07 mg/g	1041:1050	Also, the maximum adsorption capacity of monolayer for CV dye adsorption was determined using AM-SA hydrogel and AM-GO-SA nanocomposite hydrogel 62.07 mg/g and 100.30 mg/g, respectively.
33418222	8	32	theme	adsorbents	1194:1203	arg1	types					1185:1189	both types	1180:1189	both types of adsorbents	1180:1203	In addition, the parameters RL, n, and E showed that the processes of adsorption of CV dye using both types of adsorbents are physical and desirable.
33418222	1	33	theme	bonded	204:209	arg1	hydrogel					252:259	acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel	178:259	acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel	178:259	The synthesis of acrylamide bonded sodium alginate (AM-SA) hydrogel and acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel was successfully performed using the free radical method.
33418222	5	34	theme	AM-GO-SA	759:766	arg1	hydrogel					782:789	AM-GO-SA nanocomposite hydrogel	759:789	AM-GO-SA nanocomposite hydrogel	759:789	The amount of surface area was computed to be 44.689 m2/g, 0.0392 m2/g, and 6.983 m2/g for GO, AM-SA, and AM-GO-SA nanocomposite hydrogel, respectively.
33418222	1	35	theme	radical	303:309	arg1	method					311:316	the free radical method	294:316	the free radical method	294:316	The synthesis of acrylamide bonded sodium alginate (AM-SA) hydrogel and acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel was successfully performed using the free radical method.
33418222	2	36	theme	AM-SA	323:327	arg1	adsorbents					379:388	composited adsorbents	368:388	composited adsorbents in crystal violet (CV) dye removal	368:423	The AM-SA and AM-GO-SA hydrogels were applied as composited adsorbents in crystal violet (CV) dye removal.
33418222	2	36	theme	AM-SA	323:327	arg1	hydrogels					342:350	The AM-SA and AM-GO-SA hydrogels	319:350	The AM-SA and AM-GO-SA hydrogels	319:350	The AM-SA and AM-GO-SA hydrogels were applied as composited adsorbents in crystal violet (CV) dye removal.
33418222	12	37	theme	absorb	1575:1580	arg1	dye					1585:1587	absorb CV dye	1575:1587	absorb CV dye	1575:1587	Also, the Elovich model showed that AM-GO-SA nanocomposite hydrogel has more ability to absorb CV dye.
33418222	1	38	theme	sodium	211:216	arg1	hydrogel					252:259	acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel	178:259	acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel	178:259	The synthesis of acrylamide bonded sodium alginate (AM-SA) hydrogel and acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel was successfully performed using the free radical method.
33418222	5	39	theme	nanocomposite	768:780	arg1	hydrogel					782:789	AM-GO-SA nanocomposite hydrogel	759:789	AM-GO-SA nanocomposite hydrogel	759:789	The amount of surface area was computed to be 44.689 m2/g, 0.0392 m2/g, and 6.983 m2/g for GO, AM-SA, and AM-GO-SA nanocomposite hydrogel, respectively.
33418222	11	40	theme	experimental	1415:1426	arg1	data					1428:1431	The experimental data	1411:1431	The experimental data	1411:1431	The experimental data followed a pseudo- second- order (PSO) kinetic model.
33418222	2	41	theme	composited	368:377	arg1	adsorbents					379:388	composited adsorbents	368:388	composited adsorbents in crystal violet (CV) dye removal	368:423	The AM-SA and AM-GO-SA hydrogels were applied as composited adsorbents in crystal violet (CV) dye removal.
33418222	2	41	theme	composited	368:377	arg1	hydrogels					342:350	The AM-SA and AM-GO-SA hydrogels	319:350	The AM-SA and AM-GO-SA hydrogels	319:350	The AM-SA and AM-GO-SA hydrogels were applied as composited adsorbents in crystal violet (CV) dye removal.
33418222	1	42	theme	alginate	218:225	arg1	hydrogel					252:259	acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel	178:259	acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel	178:259	The synthesis of acrylamide bonded sodium alginate (AM-SA) hydrogel and acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel was successfully performed using the free radical method.
33418222	0	43	theme	acrylamide/graphene	33:51	arg1	hydrogel					96:103	acrylamide/graphene oxide bonded sodium alginate nanocomposite hydrogel	33:103	acrylamide/graphene oxide bonded sodium alginate nanocomposite hydrogel	33:103	Crystal violet dye sorption over acrylamide/graphene oxide bonded sodium alginate nanocomposite hydrogel.
33418222	12	44	theme	Elovich	1497:1503	arg1	model					1505:1509	the Elovich model	1493:1509	the Elovich model	1493:1509	Also, the Elovich model showed that AM-GO-SA nanocomposite hydrogel has more ability to absorb CV dye.
33418222	7	45	theme	nanocomposite	1018:1030	arg1	62.07 mg/g					1041:1050	AM-SA hydrogel and AM-GO-SA nanocomposite hydrogel 62.07 mg/g	990:1050	62.07 mg/g	1041:1050	Also, the maximum adsorption capacity of monolayer for CV dye adsorption was determined using AM-SA hydrogel and AM-GO-SA nanocomposite hydrogel 62.07 mg/g and 100.30 mg/g, respectively.
33418222	2	46	theme	violet	401:406	arg1	removal					417:423	crystal violet (CV) dye removal	393:423	crystal violet (CV) dye removal	393:423	The AM-SA and AM-GO-SA hydrogels were applied as composited adsorbents in crystal violet (CV) dye removal.
33418222	4	47	theme	produced	633:640	arg1	hydrogels					642:650	the produced hydrogels	629:650	the produced hydrogels	629:650	The C-O, CO, and CC groups were detected in the produced hydrogels.
33418222	8	48	theme	adsorption	1153:1162	arg1	processes					1140:1148	the processes	1136:1148	the processes of adsorption of CV dye using both types of adsorbents	1136:1203	In addition, the parameters RL, n, and E showed that the processes of adsorption of CV dye using both types of adsorbents are physical and desirable.
33418222	8	48	theme	adsorption	1153:1162	arg1	physical					1209:1216	physical	1209:1216	physical	1209:1216	In addition, the parameters RL, n, and E showed that the processes of adsorption of CV dye using both types of adsorbents are physical and desirable.
33418222	12	49	theme	AM-GO-SA	1523:1530	arg1	hydrogel					1546:1553	AM-GO-SA nanocomposite hydrogel	1523:1553	AM-GO-SA nanocomposite hydrogel	1523:1553	Also, the Elovich model showed that AM-GO-SA nanocomposite hydrogel has more ability to absorb CV dye.
33418222	5	50	theme	44.689	699:704	arg1	area					675:678	surface area	667:678	surface area	667:678	The amount of surface area was computed to be 44.689 m2/g, 0.0392 m2/g, and 6.983 m2/g for GO, AM-SA, and AM-GO-SA nanocomposite hydrogel, respectively.
33418222	5	50	theme	44.689	699:704	arg1	m2/g					706:709	44.689 m2/g	699:709	44.689 m2/g	699:709	The amount of surface area was computed to be 44.689 m2/g, 0.0392 m2/g, and 6.983 m2/g for GO, AM-SA, and AM-GO-SA nanocomposite hydrogel, respectively.
33418222	5	50	theme	44.689	699:704	arg1	amount					657:662	The amount	653:662	The amount of surface area	653:678	The amount of surface area was computed to be 44.689 m2/g, 0.0392 m2/g, and 6.983 m2/g for GO, AM-SA, and AM-GO-SA nanocomposite hydrogel, respectively.
33418222	1	51	theme	AM-GO-SA	228:235	arg1	hydrogel					252:259	acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel	178:259	acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel	178:259	The synthesis of acrylamide bonded sodium alginate (AM-SA) hydrogel and acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel was successfully performed using the free radical method.
33418222	0	52	theme	bonded	59:64	arg1	hydrogel					96:103	acrylamide/graphene oxide bonded sodium alginate nanocomposite hydrogel	33:103	acrylamide/graphene oxide bonded sodium alginate nanocomposite hydrogel	33:103	Crystal violet dye sorption over acrylamide/graphene oxide bonded sodium alginate nanocomposite hydrogel.
33418222	12	53	theme	CV	1582:1583	arg1	dye					1585:1587	absorb CV dye	1575:1587	absorb CV dye	1575:1587	Also, the Elovich model showed that AM-GO-SA nanocomposite hydrogel has more ability to absorb CV dye.
33418222	7	54	theme	AM-SA	990:994	arg1	hydrogel					996:1003	AM-SA hydrogel and AM-GO-SA nanocomposite hydrogel 62.07 mg/g	990:1050	hydrogel	996:1003	Also, the maximum adsorption capacity of monolayer for CV dye adsorption was determined using AM-SA hydrogel and AM-GO-SA nanocomposite hydrogel 62.07 mg/g and 100.30 mg/g, respectively.
33418222	1	55	theme	acrylamide	123:132	arg1	hydrogel					165:172	acrylamide bonded sodium alginate (AM-SA) hydrogel	123:172	acrylamide bonded sodium alginate (AM-SA) hydrogel	123:172	The synthesis of acrylamide bonded sodium alginate (AM-SA) hydrogel and acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel was successfully performed using the free radical method.
33418222	0	56	theme	oxide	53:57	arg1	hydrogel					96:103	acrylamide/graphene oxide bonded sodium alginate nanocomposite hydrogel	33:103	acrylamide/graphene oxide bonded sodium alginate nanocomposite hydrogel	33:103	Crystal violet dye sorption over acrylamide/graphene oxide bonded sodium alginate nanocomposite hydrogel.
33418222	3	57	theme	acquired	500:507	arg1	data					509:512	the acquired data	496:512	the acquired data	496:512	The adsorption process experiments were performed discontinuously and the acquired data showed that the efficiency is more dependent on pH than other factors.
33418222	4	58	located	detected	617:624	arg2	groups					605:610	The C-O, CO, and CC groups	585:610	The C-O, CO, and CC groups	585:610	The C-O, CO, and CC groups were detected in the produced hydrogels.
33418222	4	58	located	detected	617:624	arg1	hydrogels					642:650	the produced hydrogels	629:650	the produced hydrogels	629:650	The C-O, CO, and CC groups were detected in the produced hydrogels.
33418222	13	59	theme	obtained	1618:1625	arg1	results					1627:1633	the obtained results	1614:1633	the obtained results	1614:1633	Therefore, according to the obtained results, it can be stated that the produced hydrogels are efficient and viable composited adsorbent in removing CV dye from aqueous solution.
33418222	1	60	theme	bonded	134:139	arg1	hydrogel					165:172	acrylamide bonded sodium alginate (AM-SA) hydrogel	123:172	acrylamide bonded sodium alginate (AM-SA) hydrogel	123:172	The synthesis of acrylamide bonded sodium alginate (AM-SA) hydrogel and acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel was successfully performed using the free radical method.
33418222	12	61	theme	nanocomposite	1532:1544	arg1	hydrogel					1546:1553	AM-GO-SA nanocomposite hydrogel	1523:1553	AM-GO-SA nanocomposite hydrogel	1523:1553	Also, the Elovich model showed that AM-GO-SA nanocomposite hydrogel has more ability to absorb CV dye.
33418222	5	62	theme	area	675:678	arg1	area					675:678	surface area	667:678	surface area	667:678	The amount of surface area was computed to be 44.689 m2/g, 0.0392 m2/g, and 6.983 m2/g for GO, AM-SA, and AM-GO-SA nanocomposite hydrogel, respectively.
33418222	5	62	theme	area	675:678	arg1	m2/g					706:709	44.689 m2/g	699:709	44.689 m2/g	699:709	The amount of surface area was computed to be 44.689 m2/g, 0.0392 m2/g, and 6.983 m2/g for GO, AM-SA, and AM-GO-SA nanocomposite hydrogel, respectively.
33418222	5	62	theme	area	675:678	arg1	amount					657:662	The amount	653:662	The amount of surface area	653:678	The amount of surface area was computed to be 44.689 m2/g, 0.0392 m2/g, and 6.983 m2/g for GO, AM-SA, and AM-GO-SA nanocomposite hydrogel, respectively.
33418222	1	63	theme	sodium	141:146	arg1	hydrogel					165:172	acrylamide bonded sodium alginate (AM-SA) hydrogel	123:172	acrylamide bonded sodium alginate (AM-SA) hydrogel	123:172	The synthesis of acrylamide bonded sodium alginate (AM-SA) hydrogel and acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel was successfully performed using the free radical method.
33418222	3	64	theme	other	570:574	arg1	factors					576:582	other factors	570:582	other factors	570:582	The adsorption process experiments were performed discontinuously and the acquired data showed that the efficiency is more dependent on pH than other factors.
33418222	4	65	theme	CC	602:603	arg1	groups					605:610	The C-O, CO, and CC groups	585:610	The C-O, CO, and CC groups	585:610	The C-O, CO, and CC groups were detected in the produced hydrogels.
33418222	12	66	contain	has	1555:1557	arg2	ability					1564:1570	more ability	1559:1570	more ability	1559:1570	Also, the Elovich model showed that AM-GO-SA nanocomposite hydrogel has more ability to absorb CV dye.
33418222	12	66	contain	has	1555:1557	arg1	hydrogel					1546:1553	AM-GO-SA nanocomposite hydrogel	1523:1553	AM-GO-SA nanocomposite hydrogel	1523:1553	Also, the Elovich model showed that AM-GO-SA nanocomposite hydrogel has more ability to absorb CV dye.
33418222	7	67	theme	CV	951:952	arg1	adsorption					958:967	CV dye adsorption	951:967	CV dye adsorption	951:967	Also, the maximum adsorption capacity of monolayer for CV dye adsorption was determined using AM-SA hydrogel and AM-GO-SA nanocomposite hydrogel 62.07 mg/g and 100.30 mg/g, respectively.
33418222	1	68	theme	alginate	148:155	arg1	hydrogel					165:172	acrylamide bonded sodium alginate (AM-SA) hydrogel	123:172	acrylamide bonded sodium alginate (AM-SA) hydrogel	123:172	The synthesis of acrylamide bonded sodium alginate (AM-SA) hydrogel and acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel was successfully performed using the free radical method.
33418222	8	69	theme	dye	1170:1172	arg1	adsorption					1153:1162	adsorption	1153:1162	adsorption of CV dye using both types of adsorbents	1153:1203	In addition, the parameters RL, n, and E showed that the processes of adsorption of CV dye using both types of adsorbents are physical and desirable.
33418222	1	70	theme	nanocomposite	238:250	arg1	hydrogel					252:259	acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel	178:259	acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel	178:259	The synthesis of acrylamide bonded sodium alginate (AM-SA) hydrogel and acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel was successfully performed using the free radical method.
33418222	0	71	theme	alginate	73:80	arg1	hydrogel					96:103	acrylamide/graphene oxide bonded sodium alginate nanocomposite hydrogel	33:103	acrylamide/graphene oxide bonded sodium alginate nanocomposite hydrogel	33:103	Crystal violet dye sorption over acrylamide/graphene oxide bonded sodium alginate nanocomposite hydrogel.
33418222	2	72	theme	dye	413:415	arg1	removal					417:423	crystal violet (CV) dye removal	393:423	crystal violet (CV) dye removal	393:423	The AM-SA and AM-GO-SA hydrogels were applied as composited adsorbents in crystal violet (CV) dye removal.
33418222	7	73	theme	dye	954:956	arg1	adsorption					958:967	CV dye adsorption	951:967	CV dye adsorption	951:967	Also, the maximum adsorption capacity of monolayer for CV dye adsorption was determined using AM-SA hydrogel and AM-GO-SA nanocomposite hydrogel 62.07 mg/g and 100.30 mg/g, respectively.
33418222	2	74	theme	crystal	393:399	arg1	CV					409:410	CV	409:410	CV	409:410	The AM-SA and AM-GO-SA hydrogels were applied as composited adsorbents in crystal violet (CV) dye removal.
33418222	2	74	theme	crystal	393:399	arg1	violet					401:406	crystal violet	393:406	crystal violet (CV) dye removal	393:423	The AM-SA and AM-GO-SA hydrogels were applied as composited adsorbents in crystal violet (CV) dye removal.
33418222	1	75	theme	hydrogel	252:259	arg1	synthesis					110:118	The synthesis	106:118	The synthesis of acrylamide bonded sodium alginate (AM-SA) hydrogel and acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel	106:259	The synthesis of acrylamide bonded sodium alginate (AM-SA) hydrogel and acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel was successfully performed using the free radical method.
33418222	0	76	theme	sodium	66:71	arg1	hydrogel					96:103	acrylamide/graphene oxide bonded sodium alginate nanocomposite hydrogel	33:103	acrylamide/graphene oxide bonded sodium alginate nanocomposite hydrogel	33:103	Crystal violet dye sorption over acrylamide/graphene oxide bonded sodium alginate nanocomposite hydrogel.
33418222	13	77	theme	produced	1662:1669	arg1	efficient					1685:1693	efficient	1685:1693	efficient	1685:1693	Therefore, according to the obtained results, it can be stated that the produced hydrogels are efficient and viable composited adsorbent in removing CV dye from aqueous solution.
33418222	13	77	theme	produced	1662:1669	arg1	hydrogels					1671:1679	the produced hydrogels	1658:1679	the produced hydrogels	1658:1679	Therefore, according to the obtained results, it can be stated that the produced hydrogels are efficient and viable composited adsorbent in removing CV dye from aqueous solution.
33418222	7	78	theme	monolayer	937:945	arg1	capacity					925:932	the maximum adsorption capacity	902:932	the maximum adsorption capacity of monolayer for CV dye adsorption	902:967	Also, the maximum adsorption capacity of monolayer for CV dye adsorption was determined using AM-SA hydrogel and AM-GO-SA nanocomposite hydrogel 62.07 mg/g and 100.30 mg/g, respectively.
33418222	1	79	theme	AM-SA	158:162	arg1	hydrogel					165:172	acrylamide bonded sodium alginate (AM-SA) hydrogel	123:172	acrylamide bonded sodium alginate (AM-SA) hydrogel	123:172	The synthesis of acrylamide bonded sodium alginate (AM-SA) hydrogel and acrylamide/graphene oxide bonded sodium alginate (AM-GO-SA) nanocomposite hydrogel was successfully performed using the free radical method.
34237616	5	0	theme	-α-l-Araf-	806:815	arg1	-β-d-Manp-					918:927	contain → 5)-α-l-Araf-(1→, →2,3,5)-α-l-Araf-(1→, →4)-β-l-Arap-(1→, →4)-β-d-Galp-(1→, →6)-β-d-Galp-(1→, →4)-β-d-Manp-(1→, →6)-β-d-Manp-(1→, and terminal α-l-Araf, β-d-Galp, and β-d-Glcp residues	794:986	-β-d-Manp-	918:927	SRP70-1 was found to contain → 5)-α-l-Araf-(1→, →2,3,5)-α-l-Araf-(1→, →4)-β-l-Arap-(1→, →4)-β-d-Galp-(1→, →6)-β-d-Galp-(1→, →4)-β-d-Manp-(1→, →6)-β-d-Manp-(1→, and terminal α-l-Araf, β-d-Galp, and β-d-Glcp residues.
34237616	3	1	attach	derived	355:361	arg2	SRP70-1					343:349	SRP70-1	343:349	SRP70-1	343:349	Herein, SRP70-1 was derived from S. rebaudiana.
34237616	3	1	attach	derived	355:361	arg1	rebaudiana					371:380	rebaudiana	371:380	rebaudiana	371:380	Herein, SRP70-1 was derived from S. rebaudiana.
34237616	4	2	theme	chromatography-mass	524:542	arg1	spectrometry					544:555	gas chromatography-mass spectrometry	520:555	gas chromatography-mass spectrometry	520:555	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	4	3	theme	molecular	744:752	arg1	weight					754:759	an absolute molecular weight	732:759	an absolute molecular weight of 7698 Da	732:770	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	0	4	from	elucidation	11:21	arg1	rebaudiana					87:96	Stevia rebaudiana	80:96	Stevia rebaudiana	80:96	Structural elucidation and immunomodulatory evaluation of a polysaccharide from Stevia rebaudiana leaves.
34237616	4	5	theme	absolute	735:742	arg1	weight					754:759	an absolute molecular weight	732:759	an absolute molecular weight of 7698 Da	732:770	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	4	6	theme	nuclear	562:568	arg1	resonance					579:587	nuclear magnetic resonance	562:587	nuclear magnetic resonance spectroscopy	562:600	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	5	7	theme	-β-d-Manp-	900:909	arg1	-β-d-Manp-					918:927	contain → 5)-α-l-Araf-(1→, →2,3,5)-α-l-Araf-(1→, →4)-β-l-Arap-(1→, →4)-β-d-Galp-(1→, →6)-β-d-Galp-(1→, →4)-β-d-Manp-(1→, →6)-β-d-Manp-(1→, and terminal α-l-Araf, β-d-Galp, and β-d-Glcp residues	794:986	-β-d-Manp-	918:927	SRP70-1 was found to contain → 5)-α-l-Araf-(1→, →2,3,5)-α-l-Araf-(1→, →4)-β-l-Arap-(1→, →4)-β-d-Galp-(1→, →6)-β-d-Galp-(1→, →4)-β-d-Manp-(1→, →6)-β-d-Manp-(1→, and terminal α-l-Araf, β-d-Galp, and β-d-Glcp residues.
34237616	4	8	dep	analysis	394:401	arg1	analysis					431:438	monosaccharide composition analysis	404:438	monosaccharide composition analysis	404:438	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	4	8	dep	analysis	394:401	arg1	spectrometry					544:555	gas chromatography-mass spectrometry	520:555	gas chromatography-mass spectrometry	520:555	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	4	8	dep	analysis	394:401	arg1	spectroscopy					589:600	nuclear magnetic resonance spectroscopy	562:600	nuclear magnetic resonance spectroscopy	562:600	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	4	8	dep	analysis	394:401	arg1	detection					509:517	high-performance liquid chromatography-multi-angle light scattering detection	441:517	high-performance liquid chromatography-multi-angle light scattering detection	441:517	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	4	9	theme	gas	520:522	arg1	spectrometry					544:555	gas chromatography-mass spectrometry	520:555	gas chromatography-mass spectrometry	520:555	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	6	10	theme	nitric	1095:1100	arg1	oxide					1102:1106	nitric oxide	1095:1106	nitric oxide	1095:1106	Cell experiments showed that SRP70-1 could significantly promote phagocytosis and increase the release of nitric oxide and cytokines including IL-1β, IL-6, and TNF-α.
34237616	4	11	theme	composition	419:429	arg1	analysis					431:438	monosaccharide composition analysis	404:438	monosaccharide composition analysis	404:438	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	4	12	theme	chromatography-multi-angle	465:490	arg1	detection					509:517	high-performance liquid chromatography-multi-angle light scattering detection	441:517	high-performance liquid chromatography-multi-angle light scattering detection	441:517	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	4	13	theme	7698 Da	764:770	arg1	weight					754:759	an absolute molecular weight	732:759	an absolute molecular weight of 7698 Da	732:770	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	5	14	theme	-β-l-Arap-	846:855	arg1	-β-d-Manp-					918:927	contain → 5)-α-l-Araf-(1→, →2,3,5)-α-l-Araf-(1→, →4)-β-l-Arap-(1→, →4)-β-d-Galp-(1→, →6)-β-d-Galp-(1→, →4)-β-d-Manp-(1→, →6)-β-d-Manp-(1→, and terminal α-l-Araf, β-d-Galp, and β-d-Glcp residues	794:986	-β-d-Manp-	918:927	SRP70-1 was found to contain → 5)-α-l-Araf-(1→, →2,3,5)-α-l-Araf-(1→, →4)-β-l-Arap-(1→, →4)-β-d-Galp-(1→, →6)-β-d-Galp-(1→, →4)-β-d-Manp-(1→, →6)-β-d-Manp-(1→, and terminal α-l-Araf, β-d-Galp, and β-d-Glcp residues.
34237616	7	15	theme	Further	1156:1162	arg1	experiments					1174:1184	Further zebrafish experiments	1156:1184	Further zebrafish experiments	1156:1184	Further zebrafish experiments confirmed the immunological enhancement effects of SRP70-1.
34237616	4	16	theme	monosaccharide	404:417	arg1	analysis					431:438	monosaccharide composition analysis	404:438	monosaccharide composition analysis	404:438	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	1	17	theme	extensive	177:185	arg1	attention					187:195	extensive attention	177:195	extensive attention	177:195	Stevia rebaudiana, a sweetener with medicinal functions, has attracted extensive attention due to its application in food and pharmaceutical fields.
34237616	5	18	theme	-α-l-Araf-	828:837	arg1	-β-d-Manp-					918:927	contain → 5)-α-l-Araf-(1→, →2,3,5)-α-l-Araf-(1→, →4)-β-l-Arap-(1→, →4)-β-d-Galp-(1→, →6)-β-d-Galp-(1→, →4)-β-d-Manp-(1→, →6)-β-d-Manp-(1→, and terminal α-l-Araf, β-d-Galp, and β-d-Glcp residues	794:986	-β-d-Manp-	918:927	SRP70-1 was found to contain → 5)-α-l-Araf-(1→, →2,3,5)-α-l-Araf-(1→, →4)-β-l-Arap-(1→, →4)-β-d-Galp-(1→, →6)-β-d-Galp-(1→, →4)-β-d-Manp-(1→, →6)-β-d-Manp-(1→, and terminal α-l-Araf, β-d-Galp, and β-d-Glcp residues.
34237616	0	19	from	evaluation	44:53	arg1	rebaudiana					87:96	Stevia rebaudiana	80:96	Stevia rebaudiana	80:96	Structural elucidation and immunomodulatory evaluation of a polysaccharide from Stevia rebaudiana leaves.
34237616	7	20	theme	enhancement	1214:1224	arg1	effects					1226:1232	the immunological enhancement effects	1196:1232	the immunological enhancement effects of SRP70-1	1196:1243	Further zebrafish experiments confirmed the immunological enhancement effects of SRP70-1.
34237616	4	21	from	ratio	697:701	arg1	galactose					659:667	galactose	659:667	galactose	659:667	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	4	21	from	ratio	697:701	arg1	arabinose					674:682	arabinose	674:682	arabinose	674:682	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	4	21	from	ratio	697:701	arg1	glucose					650:656	glucose	650:656	glucose	650:656	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	4	21	from	ratio	697:701	arg1	mannose					641:647	mannose	641:647	mannose	641:647	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	0	22	theme	Structural	0:9	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation	0:21	Structural elucidation and immunomodulatory evaluation of a polysaccharide from Stevia rebaudiana leaves.
34237616	1	23	with	sweetener	127:135	arg1	functions					152:160	medicinal functions	142:160	medicinal functions	142:160	Stevia rebaudiana, a sweetener with medicinal functions, has attracted extensive attention due to its application in food and pharmaceutical fields.
34237616	4	24	theme	Structural	383:392	arg1	analysis					394:401	Structural analysis	383:401	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy)	383:601	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	1	25	theme	Stevia	106:111	arg1	rebaudiana					113:122	Stevia rebaudiana	106:122	Stevia rebaudiana	106:122	Stevia rebaudiana, a sweetener with medicinal functions, has attracted extensive attention due to its application in food and pharmaceutical fields.
34237616	1	25	theme	Stevia	106:111	arg1	sweetener					127:135	a sweetener	125:135	a sweetener with medicinal functions	125:160	Stevia rebaudiana, a sweetener with medicinal functions, has attracted extensive attention due to its application in food and pharmaceutical fields.
34237616	4	26	theme	1.35:1.00:3.23:3.47	706:724	arg1	ratio					697:701	the molar ratio	687:701	the molar ratio of 1.35:1.00:3.23:3.47	687:724	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	0	27	theme	immunomodulatory	27:42	arg1	evaluation					44:53	immunomodulatory evaluation	27:53	immunomodulatory evaluation	27:53	Structural elucidation and immunomodulatory evaluation of a polysaccharide from Stevia rebaudiana leaves.
34237616	8	28	theme	foods	1327:1331	arg1	development					1301:1311	the development	1297:1311	the development of functional foods	1297:1331	This study revealed that SRP70-1 may be useful for the development of functional foods.
34237616	4	29	theme	light	492:496	arg1	detection					509:517	high-performance liquid chromatography-multi-angle light scattering detection	441:517	high-performance liquid chromatography-multi-angle light scattering detection	441:517	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	6	30	theme	Cell	989:992	arg1	experiments					994:1004	Cell experiments	989:1004	Cell experiments	989:1004	Cell experiments showed that SRP70-1 could significantly promote phagocytosis and increase the release of nitric oxide and cytokines including IL-1β, IL-6, and TNF-α.
34237616	7	31	theme	SRP70-1	1237:1243	arg1	effects					1226:1232	the immunological enhancement effects	1196:1232	the immunological enhancement effects of SRP70-1	1196:1243	Further zebrafish experiments confirmed the immunological enhancement effects of SRP70-1.
34237616	0	32	from	rebaudiana	87:96	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation	0:21	Structural elucidation and immunomodulatory evaluation of a polysaccharide from Stevia rebaudiana leaves.
34237616	0	32	from	rebaudiana	87:96	arg1	evaluation					44:53	immunomodulatory evaluation	27:53	immunomodulatory evaluation	27:53	Structural elucidation and immunomodulatory evaluation of a polysaccharide from Stevia rebaudiana leaves.
34237616	0	32	from	rebaudiana	87:96	arg1	polysaccharide					60:73	a polysaccharide	58:73	a polysaccharide from Stevia rebaudiana	58:96	Structural elucidation and immunomodulatory evaluation of a polysaccharide from Stevia rebaudiana leaves.
34237616	8	33	theme	functional	1316:1325	arg1	foods					1327:1331	functional foods	1316:1331	functional foods	1316:1331	This study revealed that SRP70-1 may be useful for the development of functional foods.
34237616	4	34	theme	molar	691:695	arg1	ratio					697:701	the molar ratio	687:701	the molar ratio of 1.35:1.00:3.23:3.47	687:724	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	4	35	theme	magnetic	570:577	arg1	resonance					579:587	nuclear magnetic resonance	562:587	nuclear magnetic resonance spectroscopy	562:600	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	1	36	theme	food	223:226	arg1	fields					247:252	food and pharmaceutical fields	223:252	food and pharmaceutical fields	223:252	Stevia rebaudiana, a sweetener with medicinal functions, has attracted extensive attention due to its application in food and pharmaceutical fields.
34237616	0	37	theme	polysaccharide	60:73	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation	0:21	Structural elucidation and immunomodulatory evaluation of a polysaccharide from Stevia rebaudiana leaves.
34237616	0	37	theme	polysaccharide	60:73	arg1	evaluation					44:53	immunomodulatory evaluation	27:53	immunomodulatory evaluation	27:53	Structural elucidation and immunomodulatory evaluation of a polysaccharide from Stevia rebaudiana leaves.
34237616	4	38	theme	resonance	579:587	arg1	spectroscopy					589:600	nuclear magnetic resonance spectroscopy	562:600	nuclear magnetic resonance spectroscopy	562:600	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	4	39	theme	scattering	498:507	arg1	detection					509:517	high-performance liquid chromatography-multi-angle light scattering detection	441:517	high-performance liquid chromatography-multi-angle light scattering detection	441:517	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	5	40	theme	terminal	937:944	arg1	α-l-Araf					946:953	terminal α-l-Araf	937:953	terminal α-l-Araf	937:953	SRP70-1 was found to contain → 5)-α-l-Araf-(1→, →2,3,5)-α-l-Araf-(1→, →4)-β-l-Arap-(1→, →4)-β-d-Galp-(1→, →6)-β-d-Galp-(1→, →4)-β-d-Manp-(1→, →6)-β-d-Manp-(1→, and terminal α-l-Araf, β-d-Galp, and β-d-Glcp residues.
34237616	7	41	theme	immunological	1200:1212	arg1	effects					1226:1232	the immunological enhancement effects	1196:1232	the immunological enhancement effects of SRP70-1	1196:1243	Further zebrafish experiments confirmed the immunological enhancement effects of SRP70-1.
34237616	2	42	theme	few	266:268	arg1	studies					270:276	a few studies	264:276	a few studies	264:276	However, a few studies were performed to explore polysaccharides in this plant.
34237616	1	43	from	application	208:218	arg1	fields					247:252	food and pharmaceutical fields	223:252	food and pharmaceutical fields	223:252	Stevia rebaudiana, a sweetener with medicinal functions, has attracted extensive attention due to its application in food and pharmaceutical fields.
34237616	5	44	theme	-β-d-Galp-	882:891	arg1	-β-d-Manp-					918:927	contain → 5)-α-l-Araf-(1→, →2,3,5)-α-l-Araf-(1→, →4)-β-l-Arap-(1→, →4)-β-d-Galp-(1→, →6)-β-d-Galp-(1→, →4)-β-d-Manp-(1→, →6)-β-d-Manp-(1→, and terminal α-l-Araf, β-d-Galp, and β-d-Glcp residues	794:986	-β-d-Manp-	918:927	SRP70-1 was found to contain → 5)-α-l-Araf-(1→, →2,3,5)-α-l-Araf-(1→, →4)-β-l-Arap-(1→, →4)-β-d-Galp-(1→, →6)-β-d-Galp-(1→, →4)-β-d-Manp-(1→, →6)-β-d-Manp-(1→, and terminal α-l-Araf, β-d-Galp, and β-d-Glcp residues.
34237616	4	45	theme	liquid	458:463	arg1	detection					509:517	high-performance liquid chromatography-multi-angle light scattering detection	441:517	high-performance liquid chromatography-multi-angle light scattering detection	441:517	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	6	46	theme	oxide	1102:1106	arg1	release					1084:1090	the release	1080:1090	the release of nitric oxide and cytokines including IL-1β, IL-6, and TNF-α	1080:1153	Cell experiments showed that SRP70-1 could significantly promote phagocytosis and increase the release of nitric oxide and cytokines including IL-1β, IL-6, and TNF-α.
34237616	5	47	theme	β-d-Glcp	970:977	arg1	residues					979:986	β-d-Glcp residues	970:986	β-d-Glcp residues	970:986	SRP70-1 was found to contain → 5)-α-l-Araf-(1→, →2,3,5)-α-l-Araf-(1→, →4)-β-l-Arap-(1→, →4)-β-d-Galp-(1→, →6)-β-d-Galp-(1→, →4)-β-d-Manp-(1→, →6)-β-d-Manp-(1→, and terminal α-l-Araf, β-d-Galp, and β-d-Glcp residues.
34237616	1	48	theme	medicinal	142:150	arg1	functions					152:160	medicinal functions	142:160	medicinal functions	142:160	Stevia rebaudiana, a sweetener with medicinal functions, has attracted extensive attention due to its application in food and pharmaceutical fields.
34237616	1	49	theme	pharmaceutical	232:245	arg1	fields					247:252	food and pharmaceutical fields	223:252	food and pharmaceutical fields	223:252	Stevia rebaudiana, a sweetener with medicinal functions, has attracted extensive attention due to its application in food and pharmaceutical fields.
34237616	4	50	theme	high-performance	441:456	arg1	detection					509:517	high-performance liquid chromatography-multi-angle light scattering detection	441:517	high-performance liquid chromatography-multi-angle light scattering detection	441:517	Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da.
34237616	6	51	theme	cytokines	1112:1120	arg1	release					1084:1090	the release	1080:1090	the release of nitric oxide and cytokines including IL-1β, IL-6, and TNF-α	1080:1153	Cell experiments showed that SRP70-1 could significantly promote phagocytosis and increase the release of nitric oxide and cytokines including IL-1β, IL-6, and TNF-α.
34237616	5	52	theme	-β-d-Galp-	864:873	arg1	-β-d-Manp-					918:927	contain → 5)-α-l-Araf-(1→, →2,3,5)-α-l-Araf-(1→, →4)-β-l-Arap-(1→, →4)-β-d-Galp-(1→, →6)-β-d-Galp-(1→, →4)-β-d-Manp-(1→, →6)-β-d-Manp-(1→, and terminal α-l-Araf, β-d-Galp, and β-d-Glcp residues	794:986	-β-d-Manp-	918:927	SRP70-1 was found to contain → 5)-α-l-Araf-(1→, →2,3,5)-α-l-Araf-(1→, →4)-β-l-Arap-(1→, →4)-β-d-Galp-(1→, →6)-β-d-Galp-(1→, →4)-β-d-Manp-(1→, →6)-β-d-Manp-(1→, and terminal α-l-Araf, β-d-Galp, and β-d-Glcp residues.
34237616	7	53	theme	zebrafish	1164:1172	arg1	experiments					1174:1184	Further zebrafish experiments	1156:1184	Further zebrafish experiments	1156:1184	Further zebrafish experiments confirmed the immunological enhancement effects of SRP70-1.
34399523	2	0	theme	1 → 6	460:464	arg1	G4Sα					486:489	as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα	440:489	as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→	440:492	PHP1 was mainly composed of galactose and the main linkage types were identified as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→.
34399523	4	1	from	abundance	728:736	arg1	rats					774:777	rats	774:777	rats	774:777	PHP1 promoted the growth of both propionic acid-producing bacteria and propionic acid production, as well as influencing the composition and abundance of beneficial microbiota species in rats, which may be related to its high level of sulfation.
34399523	2	2	theme	LA	477:478	arg1	G4Sα					486:489	as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα	440:489	as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→	440:492	PHP1 was mainly composed of galactose and the main linkage types were identified as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→.
34399523	6	3	theme	in	1209:1210	arg1	regulation					1184:1193	its regulation	1180:1193	its regulation of microbiota in vivo	1180:1215	These results provided new insights into the structure-activity relationships between P. haitanensis polysaccharide and its regulation of microbiota in vivo.
34399523	4	4	theme	microbiota	752:761	arg1	species					763:769	beneficial microbiota species	741:769	beneficial microbiota species in rats, which may be related to its high level of sulfation	741:830	PHP1 promoted the growth of both propionic acid-producing bacteria and propionic acid production, as well as influencing the composition and abundance of beneficial microbiota species in rats, which may be related to its high level of sulfation.
34399523	5	5	theme	PHP1	857:860	arg1	weight					847:852	The molecular weight	833:852	The molecular weight of PHP1	833:860	The molecular weight of PHP1 decreased significantly after fermentation, which may result from hydrolysis of the galactan (with α- and β-linkages between galactose residues) by α- or β-galactosidase secreted by the microbiota.
34399523	0	6	theme	polysaccharide	88:101	arg1	microbiota					72:81	rat intestinal microbiota	57:81	rat intestinal microbiota of a polysaccharide from Porphyra haitanensis	57:127	Structural characterization and in vitro fermentation by rat intestinal microbiota of a polysaccharide from Porphyra haitanensis.
34399523	6	7	theme	microbiota	1198:1207	arg1	regulation					1184:1193	its regulation	1180:1193	its regulation of microbiota in vivo	1180:1215	These results provided new insights into the structure-activity relationships between P. haitanensis polysaccharide and its regulation of microbiota in vivo.
34399523	6	7	theme	microbiota	1198:1207	arg1	polysaccharide					1161:1174	P. haitanensis polysaccharide	1146:1174	P. haitanensis polysaccharide	1146:1174	These results provided new insights into the structure-activity relationships between P. haitanensis polysaccharide and its regulation of microbiota in vivo.
34399523	6	8	theme	new	1083:1085	arg1	insights					1087:1094	new insights	1083:1094	new insights into the structure-activity relationships between P. haitanensis polysaccharide and its regulation of microbiota in vivo	1083:1215	These results provided new insights into the structure-activity relationships between P. haitanensis polysaccharide and its regulation of microbiota in vivo.
34399523	1	9	theme	rat	266:268	arg1	fermentations					287:299	rat fecal microbiota fermentations	266:299	rat fecal microbiota fermentations	266:299	A sulfated polysaccharide (PHP1) produced by the marine red alga Porphyra haitanensis was structurally characterized, and its effect on rat fecal microbiota fermentations and short chain fatty acids production were investigated.
34399523	2	10	theme	1 → 4	471:475	arg1	G4Sα					486:489	as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα	440:489	as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→	440:492	PHP1 was mainly composed of galactose and the main linkage types were identified as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→.
34399523	4	11	from	rats	774:777	arg1	composition					712:722	composition	712:722	composition	712:722	PHP1 promoted the growth of both propionic acid-producing bacteria and propionic acid production, as well as influencing the composition and abundance of beneficial microbiota species in rats, which may be related to its high level of sulfation.
34399523	4	11	from	rats	774:777	arg1	abundance					728:736	abundance	728:736	abundance	728:736	PHP1 promoted the growth of both propionic acid-producing bacteria and propionic acid production, as well as influencing the composition and abundance of beneficial microbiota species in rats, which may be related to its high level of sulfation.
34399523	4	12	theme	species	763:769	arg1	composition					712:722	composition	712:722	composition	712:722	PHP1 promoted the growth of both propionic acid-producing bacteria and propionic acid production, as well as influencing the composition and abundance of beneficial microbiota species in rats, which may be related to its high level of sulfation.
34399523	4	12	theme	species	763:769	arg1	abundance					728:736	abundance	728:736	abundance	728:736	PHP1 promoted the growth of both propionic acid-producing bacteria and propionic acid production, as well as influencing the composition and abundance of beneficial microbiota species in rats, which may be related to its high level of sulfation.
34399523	5	13	theme	molecular	837:845	arg1	weight					847:852	The molecular weight	833:852	The molecular weight of PHP1	833:860	The molecular weight of PHP1 decreased significantly after fermentation, which may result from hydrolysis of the galactan (with α- and β-linkages between galactose residues) by α- or β-galactosidase secreted by the microbiota.
34399523	5	14	with	galactan	946:953	arg1	β-linkages					968:977	β-linkages	968:977	β-linkages	968:977	The molecular weight of PHP1 decreased significantly after fermentation, which may result from hydrolysis of the galactan (with α- and β-linkages between galactose residues) by α- or β-galactosidase secreted by the microbiota.
34399523	5	14	with	galactan	946:953	arg1	α-					961:962	α-	961:962	α-	961:962	The molecular weight of PHP1 decreased significantly after fermentation, which may result from hydrolysis of the galactan (with α- and β-linkages between galactose residues) by α- or β-galactosidase secreted by the microbiota.
34399523	2	15	dep	G4Sα	486:489	arg1	1→					491:492	1→	491:492	as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→	440:492	PHP1 was mainly composed of galactose and the main linkage types were identified as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→.
34399523	2	16	theme	G4Sβ	447:450	arg1	G4Sα					486:489	as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα	440:489	as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→	440:492	PHP1 was mainly composed of galactose and the main linkage types were identified as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→.
34399523	4	17	from	species	763:769	arg1	rats					774:777	rats	774:777	rats	774:777	PHP1 promoted the growth of both propionic acid-producing bacteria and propionic acid production, as well as influencing the composition and abundance of beneficial microbiota species in rats, which may be related to its high level of sulfation.
34399523	4	18	theme	propionic	658:666	arg1	acid					668:671	propionic acid	658:671	propionic acid production	658:682	PHP1 promoted the growth of both propionic acid-producing bacteria and propionic acid production, as well as influencing the composition and abundance of beneficial microbiota species in rats, which may be related to its high level of sulfation.
34399523	1	19	theme	marine	179:184	arg1	alga					190:193	the marine red alga	175:193	the marine red alga	175:193	A sulfated polysaccharide (PHP1) produced by the marine red alga Porphyra haitanensis was structurally characterized, and its effect on rat fecal microbiota fermentations and short chain fatty acids production were investigated.
34399523	0	20	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and in vitro fermentation by rat intestinal microbiota of a polysaccharide from Porphyra haitanensis.
34399523	1	21	theme	fecal	270:274	arg1	fermentations					287:299	rat fecal microbiota fermentations	266:299	rat fecal microbiota fermentations	266:299	A sulfated polysaccharide (PHP1) produced by the marine red alga Porphyra haitanensis was structurally characterized, and its effect on rat fecal microbiota fermentations and short chain fatty acids production were investigated.
34399523	6	22	dep	in	1209:1210	arg1	vivo					1212:1215	vivo	1212:1215	vivo	1212:1215	These results provided new insights into the structure-activity relationships between P. haitanensis polysaccharide and its regulation of microbiota in vivo.
34399523	6	23	theme	haitanensis	1149:1159	arg1	polysaccharide					1161:1174	P. haitanensis polysaccharide	1146:1174	P. haitanensis polysaccharide	1146:1174	These results provided new insights into the structure-activity relationships between P. haitanensis polysaccharide and its regulation of microbiota in vivo.
34399523	5	24	theme	galactan	946:953	arg1	hydrolysis					928:937	hydrolysis	928:937	hydrolysis of the galactan (with α- and β-linkages between galactose residues) by α- or β-galactosidase secreted by the microbiota	928:1057	The molecular weight of PHP1 decreased significantly after fermentation, which may result from hydrolysis of the galactan (with α- and β-linkages between galactose residues) by α- or β-galactosidase secreted by the microbiota.
34399523	1	25	theme	red	186:188	arg1	alga					190:193	the marine red alga	175:193	the marine red alga	175:193	A sulfated polysaccharide (PHP1) produced by the marine red alga Porphyra haitanensis was structurally characterized, and its effect on rat fecal microbiota fermentations and short chain fatty acids production were investigated.
34399523	0	26	theme	in	32:33	arg1	fermentation					41:52	in vitro fermentation	32:52	in vitro fermentation	32:52	Structural characterization and in vitro fermentation by rat intestinal microbiota of a polysaccharide from Porphyra haitanensis.
34399523	1	27	theme	microbiota	276:285	arg1	fermentations					287:299	rat fecal microbiota fermentations	266:299	rat fecal microbiota fermentations	266:299	A sulfated polysaccharide (PHP1) produced by the marine red alga Porphyra haitanensis was structurally characterized, and its effect on rat fecal microbiota fermentations and short chain fatty acids production were investigated.
34399523	4	28	theme	sulfation	822:830	arg1	level					813:817	its high level	804:817	its high level of sulfation	804:830	PHP1 promoted the growth of both propionic acid-producing bacteria and propionic acid production, as well as influencing the composition and abundance of beneficial microbiota species in rats, which may be related to its high level of sulfation.
34399523	6	29	theme	P.	1146:1147	arg1	polysaccharide					1161:1174	P. haitanensis polysaccharide	1146:1174	P. haitanensis polysaccharide	1146:1174	These results provided new insights into the structure-activity relationships between P. haitanensis polysaccharide and its regulation of microbiota in vivo.
34399523	1	30	theme	fermentations	287:299	arg1	production					329:338	rat fecal microbiota fermentations and short chain fatty acids production	266:338	rat fecal microbiota fermentations and short chain fatty acids production	266:338	A sulfated polysaccharide (PHP1) produced by the marine red alga Porphyra haitanensis was structurally characterized, and its effect on rat fecal microbiota fermentations and short chain fatty acids production were investigated.
34399523	4	31	theme	acid	668:671	arg1	production					673:682	propionic acid production	658:682	propionic acid production	658:682	PHP1 promoted the growth of both propionic acid-producing bacteria and propionic acid production, as well as influencing the composition and abundance of beneficial microbiota species in rats, which may be related to its high level of sulfation.
34399523	2	32	theme	1 → 3	452:456	arg1	G4Sα					486:489	as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα	440:489	as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→	440:492	PHP1 was mainly composed of galactose and the main linkage types were identified as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→.
34399523	2	33	theme	G	458:458	arg1	G4Sα					486:489	as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα	440:489	as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→	440:492	PHP1 was mainly composed of galactose and the main linkage types were identified as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→.
34399523	2	34	theme	1 → 6	480:484	arg1	G4Sα					486:489	as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα	440:489	as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→	440:492	PHP1 was mainly composed of galactose and the main linkage types were identified as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→.
34399523	2	35	theme	linkage	410:416	arg1	types					418:422	the main linkage types	401:422	the main linkage types	401:422	PHP1 was mainly composed of galactose and the main linkage types were identified as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→.
34399523	1	36	theme	short	305:309	arg1	acids					323:327	short chain fatty acids	305:327	short chain fatty acids	305:327	A sulfated polysaccharide (PHP1) produced by the marine red alga Porphyra haitanensis was structurally characterized, and its effect on rat fecal microbiota fermentations and short chain fatty acids production were investigated.
34399523	1	37	from	effect	256:261	arg1	production					329:338	rat fecal microbiota fermentations and short chain fatty acids production	266:338	rat fecal microbiota fermentations and short chain fatty acids production	266:338	A sulfated polysaccharide (PHP1) produced by the marine red alga Porphyra haitanensis was structurally characterized, and its effect on rat fecal microbiota fermentations and short chain fatty acids production were investigated.
34399523	0	38	from	microbiota	72:81	arg1	haitanensis					117:127	haitanensis	117:127	haitanensis	117:127	Structural characterization and in vitro fermentation by rat intestinal microbiota of a polysaccharide from Porphyra haitanensis.
34399523	2	39	theme	as → 3	440:445	arg1	G4Sα					486:489	as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα	440:489	as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→	440:492	PHP1 was mainly composed of galactose and the main linkage types were identified as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→.
34399523	2	40	theme	main	405:408	arg1	types					418:422	the main linkage types	401:422	the main linkage types	401:422	PHP1 was mainly composed of galactose and the main linkage types were identified as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→.
34399523	0	41	theme	rat	57:59	arg1	microbiota					72:81	rat intestinal microbiota	57:81	rat intestinal microbiota of a polysaccharide from Porphyra haitanensis	57:127	Structural characterization and in vitro fermentation by rat intestinal microbiota of a polysaccharide from Porphyra haitanensis.
34399523	1	42	theme	chain	311:315	arg1	acids					323:327	short chain fatty acids	305:327	short chain fatty acids	305:327	A sulfated polysaccharide (PHP1) produced by the marine red alga Porphyra haitanensis was structurally characterized, and its effect on rat fecal microbiota fermentations and short chain fatty acids production were investigated.
34399523	5	43	theme	galactose	987:995	arg1	residues					997:1004	galactose residues	987:1004	galactose residues	987:1004	The molecular weight of PHP1 decreased significantly after fermentation, which may result from hydrolysis of the galactan (with α- and β-linkages between galactose residues) by α- or β-galactosidase secreted by the microbiota.
34399523	3	44	theme	surface	499:505	arg1	morphology					507:516	The surface morphology	495:516	The surface morphology of dried PHP1 films	495:536	The surface morphology of dried PHP1 films appears to be related to its chemical structure.
34399523	3	44	theme	surface	499:505	arg1	related					552:558	related	552:558	related	552:558	The surface morphology of dried PHP1 films appears to be related to its chemical structure.
34399523	4	45	theme	propionic	620:628	arg1	bacteria					645:652	both propionic acid-producing bacteria	615:652	both propionic acid-producing bacteria	615:652	PHP1 promoted the growth of both propionic acid-producing bacteria and propionic acid production, as well as influencing the composition and abundance of beneficial microbiota species in rats, which may be related to its high level of sulfation.
34399523	1	46	theme	fatty	317:321	arg1	acids					323:327	short chain fatty acids	305:327	short chain fatty acids	305:327	A sulfated polysaccharide (PHP1) produced by the marine red alga Porphyra haitanensis was structurally characterized, and its effect on rat fecal microbiota fermentations and short chain fatty acids production were investigated.
34399523	0	47	from	haitanensis	117:127	arg1	microbiota					72:81	rat intestinal microbiota	57:81	rat intestinal microbiota of a polysaccharide from Porphyra haitanensis	57:127	Structural characterization and in vitro fermentation by rat intestinal microbiota of a polysaccharide from Porphyra haitanensis.
34399523	0	47	from	haitanensis	117:127	arg1	polysaccharide					88:101	a polysaccharide	86:101	a polysaccharide from Porphyra haitanensis	86:127	Structural characterization and in vitro fermentation by rat intestinal microbiota of a polysaccharide from Porphyra haitanensis.
34399523	4	48	theme	beneficial	741:750	arg1	species					763:769	beneficial microbiota species	741:769	beneficial microbiota species in rats, which may be related to its high level of sulfation	741:830	PHP1 promoted the growth of both propionic acid-producing bacteria and propionic acid production, as well as influencing the composition and abundance of beneficial microbiota species in rats, which may be related to its high level of sulfation.
34399523	1	49	theme	acids	323:327	arg1	production					329:338	rat fecal microbiota fermentations and short chain fatty acids production	266:338	rat fecal microbiota fermentations and short chain fatty acids production	266:338	A sulfated polysaccharide (PHP1) produced by the marine red alga Porphyra haitanensis was structurally characterized, and its effect on rat fecal microbiota fermentations and short chain fatty acids production were investigated.
34399523	0	50	dep	in	32:33	arg1	vitro					35:39	vitro	35:39	vitro	35:39	Structural characterization and in vitro fermentation by rat intestinal microbiota of a polysaccharide from Porphyra haitanensis.
34399523	3	51	theme	dried	521:525	arg1	films					532:536	dried PHP1 films	521:536	dried PHP1 films	521:536	The surface morphology of dried PHP1 films appears to be related to its chemical structure.
34399523	1	52	theme	sulfated	132:139	arg1	PHP1					157:160	PHP1	157:160	PHP1	157:160	A sulfated polysaccharide (PHP1) produced by the marine red alga Porphyra haitanensis was structurally characterized, and its effect on rat fecal microbiota fermentations and short chain fatty acids production were investigated.
34399523	1	52	theme	sulfated	132:139	arg1	polysaccharide					141:154	A sulfated polysaccharide	130:154	A sulfated polysaccharide (PHP1) produced by the marine red alga Porphyra haitanensis	130:214	A sulfated polysaccharide (PHP1) produced by the marine red alga Porphyra haitanensis was structurally characterized, and its effect on rat fecal microbiota fermentations and short chain fatty acids production were investigated.
34399523	4	53	theme	high	808:811	arg1	level					813:817	its high level	804:817	its high level of sulfation	804:830	PHP1 promoted the growth of both propionic acid-producing bacteria and propionic acid production, as well as influencing the composition and abundance of beneficial microbiota species in rats, which may be related to its high level of sulfation.
34399523	3	54	theme	chemical	567:574	arg1	structure					576:584	its chemical structure	563:584	its chemical structure	563:584	The surface morphology of dried PHP1 films appears to be related to its chemical structure.
34399523	6	55	theme	structure-activity	1105:1122	arg1	relationships					1124:1136	the structure-activity relationships	1101:1136	the structure-activity relationships between P. haitanensis polysaccharide and its regulation of microbiota in vivo	1101:1215	These results provided new insights into the structure-activity relationships between P. haitanensis polysaccharide and its regulation of microbiota in vivo.
34399523	4	56	theme	acid-producing	630:643	arg1	bacteria					645:652	both propionic acid-producing bacteria	615:652	both propionic acid-producing bacteria	615:652	PHP1 promoted the growth of both propionic acid-producing bacteria and propionic acid production, as well as influencing the composition and abundance of beneficial microbiota species in rats, which may be related to its high level of sulfation.
34399523	4	57	theme	bacteria	645:652	arg1	growth					605:610	the growth	601:610	the growth of both propionic acid-producing bacteria	601:652	PHP1 promoted the growth of both propionic acid-producing bacteria and propionic acid production, as well as influencing the composition and abundance of beneficial microbiota species in rats, which may be related to its high level of sulfation.
34399523	4	57	theme	bacteria	645:652	arg1	production					673:682	propionic acid production	658:682	propionic acid production	658:682	PHP1 promoted the growth of both propionic acid-producing bacteria and propionic acid production, as well as influencing the composition and abundance of beneficial microbiota species in rats, which may be related to its high level of sulfation.
34399523	2	58	theme	G4Sα	466:469	arg1	G4Sα					486:489	as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα	440:489	as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→	440:492	PHP1 was mainly composed of galactose and the main linkage types were identified as → 3)G4Sβ(1 → 3)G(1 → 6)G4Sα(1 → 4)LA(1 → 6)G4Sα(1→.
34399523	3	59	theme	PHP1	527:530	arg1	films					532:536	dried PHP1 films	521:536	dried PHP1 films	521:536	The surface morphology of dried PHP1 films appears to be related to its chemical structure.
34399523	4	60	from	composition	712:722	arg1	rats					774:777	rats	774:777	rats	774:777	PHP1 promoted the growth of both propionic acid-producing bacteria and propionic acid production, as well as influencing the composition and abundance of beneficial microbiota species in rats, which may be related to its high level of sulfation.
34399523	4	61	dep	composition	712:722	arg1	the					708:710	the	708:710	the	708:710	PHP1 promoted the growth of both propionic acid-producing bacteria and propionic acid production, as well as influencing the composition and abundance of beneficial microbiota species in rats, which may be related to its high level of sulfation.
34399523	0	62	theme	intestinal	61:70	arg1	microbiota					72:81	rat intestinal microbiota	57:81	rat intestinal microbiota of a polysaccharide from Porphyra haitanensis	57:127	Structural characterization and in vitro fermentation by rat intestinal microbiota of a polysaccharide from Porphyra haitanensis.
34399523	3	63	theme	films	532:536	arg1	morphology					507:516	The surface morphology	495:516	The surface morphology of dried PHP1 films	495:536	The surface morphology of dried PHP1 films appears to be related to its chemical structure.
34399523	3	63	theme	films	532:536	arg1	related					552:558	related	552:558	related	552:558	The surface morphology of dried PHP1 films appears to be related to its chemical structure.
34146785	0	0	theme	supported	72:80	arg1	membranes					90:98	supported bilayer membranes	72:98	supported bilayer membranes	72:98	Role of the interactions of soft hyaluronan nanomaterials with CD44 and supported bilayer membranes in the cellular uptake.
34146785	4	1	theme	stronger	821:828	arg1	interaction					830:840	a stronger interaction	819:840	a stronger interaction with recombinant CD44 receptor	819:871	The ability of HA-NPs to efficiently compete with native HA assessed using Bio-layer interferometry showed that NPs had a stronger interaction with recombinant CD44 receptor compared to native HA.
34146785	2	2	theme	nanoplatelets	330:342	arg1	internalization					285:299	cell internalization	280:299	cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions	280:505	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	4	3	theme	CD44	859:862	arg1	receptor					864:871	recombinant CD44 receptor	847:871	recombinant CD44 receptor	847:871	The ability of HA-NPs to efficiently compete with native HA assessed using Bio-layer interferometry showed that NPs had a stronger interaction with recombinant CD44 receptor compared to native HA.
34146785	7	4	theme	drug	1248:1251	arg1	delivery					1253:1260	intracellular drug delivery	1234:1260	intracellular drug delivery using soft HA-NPs through receptor-mediated multivalent interactions	1234:1329	Our findings provide insights into intracellular drug delivery using soft HA-NPs through receptor-mediated multivalent interactions.
34146785	1	5	theme	targeted	240:247	arg1	cells					249:253	targeted cells	240:253	targeted cells	240:253	Increasing valence by acting on nanomaterial morphology can enhance the ability of a ligand to specifically bind to targeted cells.
34146785	2	6	theme	atomic	460:465	arg1	microscopy					473:482	atomic force microscopy	460:482	atomic force microscopy in hydrated conditions	460:505	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	0	7	from	Role	0:3	arg1	uptake					116:121	the cellular uptake	103:121	the cellular uptake	103:121	Role of the interactions of soft hyaluronan nanomaterials with CD44 and supported bilayer membranes in the cellular uptake.
34146785	4	8	theme	HA-NPs	714:719	arg1	ability					703:709	The ability	699:709	The ability of HA-NPs to efficiently compete with native HA assessed using Bio-layer interferometry	699:797	The ability of HA-NPs to efficiently compete with native HA assessed using Bio-layer interferometry showed that NPs had a stronger interaction with recombinant CD44 receptor compared to native HA.
34146785	5	9	theme	NPs	960:962	arg1	properties					942:951	physical properties	933:951	physical properties of the NPs and the implication of multivalent interactions in HA binding to CD44	933:1032	The results were discussed regarding physical properties of the NPs and the implication of multivalent interactions in HA binding to CD44.
34146785	0	10	theme	bilayer	82:88	arg1	membranes					90:98	supported bilayer membranes	72:98	supported bilayer membranes	72:98	Role of the interactions of soft hyaluronan nanomaterials with CD44 and supported bilayer membranes in the cellular uptake.
34146785	6	11	theme	lipid	1166:1170	arg1	membranes					1172:1180	lipid membranes	1166:1180	lipid membranes	1166:1180	Experiments conducted on supported bilayer membranes with different compositions showed that non-specific interactions of NPs with lipid membranes were negligible.
34146785	5	12	theme	physical	933:940	arg1	properties					942:951	physical properties	933:951	physical properties of the NPs and the implication of multivalent interactions in HA binding to CD44	933:1032	The results were discussed regarding physical properties of the NPs and the implication of multivalent interactions in HA binding to CD44.
34146785	5	13	from	NPs	960:962	arg1	binding					1018:1024	HA binding	1015:1024	HA binding to CD44	1015:1032	The results were discussed regarding physical properties of the NPs and the implication of multivalent interactions in HA binding to CD44.
34146785	3	14	theme	time-dependent	675:688	arg1	manners					690:696	a dose-dependent and time-dependent manners	654:696	a dose-dependent and time-dependent manners	654:696	Fluorescence imaging revealed that internalization of HA-NPs by a T24 tumor cell line and by macrophages was higher than native polysaccharide in a dose-dependent and time-dependent manners.
34146785	6	15	theme	supported	1060:1068	arg1	membranes					1078:1086	supported bilayer membranes	1060:1086	supported bilayer membranes with different compositions	1060:1114	Experiments conducted on supported bilayer membranes with different compositions showed that non-specific interactions of NPs with lipid membranes were negligible.
34146785	1	16	theme	nanomaterial	156:167	arg1	morphology					169:178	nanomaterial morphology	156:178	nanomaterial morphology	156:178	Increasing valence by acting on nanomaterial morphology can enhance the ability of a ligand to specifically bind to targeted cells.
34146785	2	17	theme	aspect	413:418	arg1	ratio					420:424	high aspect ratio	408:424	high aspect ratio (Γ≈12 to 20)	408:437	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	2	17	theme	aspect	413:418	arg1	shape					383:387	a typical hexagonal shape	363:387	a typical hexagonal shape	363:387	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	2	17	theme	aspect	413:418	arg1	20					435:436	20	435:436	20	435:436	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	6	18	with	membranes	1078:1086	arg1	compositions					1103:1114	different compositions	1093:1114	different compositions	1093:1114	Experiments conducted on supported bilayer membranes with different compositions showed that non-specific interactions of NPs with lipid membranes were negligible.
34146785	6	19	theme	NPs	1157:1159	arg1	interactions					1141:1152	non-specific interactions	1128:1152	non-specific interactions of NPs with lipid membranes	1128:1180	Experiments conducted on supported bilayer membranes with different compositions showed that non-specific interactions of NPs with lipid membranes were negligible.
34146785	2	20	theme	high	408:411	arg1	ratio					420:424	high aspect ratio	408:424	high aspect ratio (Γ≈12 to 20)	408:437	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	2	20	theme	high	408:411	arg1	shape					383:387	a typical hexagonal shape	363:387	a typical hexagonal shape	363:387	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	2	20	theme	high	408:411	arg1	20					435:436	20	435:436	20	435:436	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	3	21	theme	dose-dependent	656:669	arg1	manners					690:696	a dose-dependent and time-dependent manners	654:696	a dose-dependent and time-dependent manners	654:696	Fluorescence imaging revealed that internalization of HA-NPs by a T24 tumor cell line and by macrophages was higher than native polysaccharide in a dose-dependent and time-dependent manners.
34146785	2	22	theme	soft	304:307	arg1	nanoplatelets					330:342	soft hyaluronic acid (HA) nanoplatelets	304:342	soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions	304:505	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	2	22	theme	soft	304:307	arg1	NPs					345:347	NPs	345:347	NPs	345:347	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	4	23	theme	recombinant	847:857	arg1	receptor					864:871	recombinant CD44 receptor	847:871	recombinant CD44 receptor	847:871	The ability of HA-NPs to efficiently compete with native HA assessed using Bio-layer interferometry showed that NPs had a stronger interaction with recombinant CD44 receptor compared to native HA.
34146785	3	24	theme	native	629:634	arg1	polysaccharide					636:649	native polysaccharide	629:649	native polysaccharide	629:649	Fluorescence imaging revealed that internalization of HA-NPs by a T24 tumor cell line and by macrophages was higher than native polysaccharide in a dose-dependent and time-dependent manners.
34146785	7	25	theme	intracellular	1234:1246	arg1	delivery					1253:1260	intracellular drug delivery	1234:1260	intracellular drug delivery using soft HA-NPs through receptor-mediated multivalent interactions	1234:1329	Our findings provide insights into intracellular drug delivery using soft HA-NPs through receptor-mediated multivalent interactions.
34146785	0	26	theme	interactions	12:23	arg1	Role					0:3	Role	0:3	Role of the interactions of soft hyaluronan nanomaterials with CD44 and supported bilayer membranes in the cellular uptake.	0:122	Role of the interactions of soft hyaluronan nanomaterials with CD44 and supported bilayer membranes in the cellular uptake.
34146785	2	27	theme	hydrated	487:494	arg1	conditions					496:505	hydrated conditions	487:505	hydrated conditions	487:505	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	5	28	theme	interactions	999:1010	arg1	NPs					960:962	the NPs	956:962	the NPs	956:962	The results were discussed regarding physical properties of the NPs and the implication of multivalent interactions in HA binding to CD44.
34146785	5	28	theme	interactions	999:1010	arg1	implication					972:982	the implication	968:982	the implication of multivalent interactions in HA binding to CD44	968:1032	The results were discussed regarding physical properties of the NPs and the implication of multivalent interactions in HA binding to CD44.
34146785	6	29	theme	non-specific	1128:1139	arg1	interactions					1141:1152	non-specific interactions	1128:1152	non-specific interactions of NPs with lipid membranes	1128:1180	Experiments conducted on supported bilayer membranes with different compositions showed that non-specific interactions of NPs with lipid membranes were negligible.
34146785	2	30	theme	flat	390:393	arg1	shape					383:387	a typical hexagonal shape	363:387	a typical hexagonal shape	363:387	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	2	30	theme	flat	390:393	arg1	surfaces					395:402	flat surfaces	390:402	flat surfaces	390:402	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	0	31	theme	cellular	107:114	arg1	uptake					116:121	the cellular uptake	103:121	the cellular uptake	103:121	Role of the interactions of soft hyaluronan nanomaterials with CD44 and supported bilayer membranes in the cellular uptake.
34146785	6	32	with	interactions	1141:1152	arg1	membranes					1172:1180	lipid membranes	1166:1180	lipid membranes	1166:1180	Experiments conducted on supported bilayer membranes with different compositions showed that non-specific interactions of NPs with lipid membranes were negligible.
34146785	2	33	theme	cell	280:283	arg1	internalization					285:299	cell internalization	280:299	cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions	280:505	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	5	34	from	implication	972:982	arg1	binding					1018:1024	HA binding	1015:1024	HA binding to CD44	1015:1032	The results were discussed regarding physical properties of the NPs and the implication of multivalent interactions in HA binding to CD44.
34146785	0	35	theme	soft	28:31	arg1	nanomaterials					44:56	soft hyaluronan nanomaterials	28:56	soft hyaluronan nanomaterials	28:56	Role of the interactions of soft hyaluronan nanomaterials with CD44 and supported bilayer membranes in the cellular uptake.
34146785	7	36	theme	multivalent	1306:1316	arg1	interactions					1318:1329	receptor-mediated multivalent interactions	1288:1329	receptor-mediated multivalent interactions	1288:1329	Our findings provide insights into intracellular drug delivery using soft HA-NPs through receptor-mediated multivalent interactions.
34146785	5	37	theme	implication	972:982	arg1	properties					942:951	physical properties	933:951	physical properties of the NPs and the implication of multivalent interactions in HA binding to CD44	933:1032	The results were discussed regarding physical properties of the NPs and the implication of multivalent interactions in HA binding to CD44.
34146785	2	38	theme	hexagonal	373:381	arg1	ratio					420:424	high aspect ratio	408:424	high aspect ratio (Γ≈12 to 20)	408:437	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	2	38	theme	hexagonal	373:381	arg1	shape					383:387	a typical hexagonal shape	363:387	a typical hexagonal shape	363:387	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	2	38	theme	hexagonal	373:381	arg1	surfaces					395:402	flat surfaces	390:402	flat surfaces	390:402	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	2	39	theme	force	467:471	arg1	microscopy					473:482	atomic force microscopy	460:482	atomic force microscopy in hydrated conditions	460:505	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	3	40	theme	tumor	578:582	arg1	line					589:592	a T24 tumor cell line	572:592	a T24 tumor cell line	572:592	Fluorescence imaging revealed that internalization of HA-NPs by a T24 tumor cell line and by macrophages was higher than native polysaccharide in a dose-dependent and time-dependent manners.
34146785	0	41	with	interactions	12:23	arg1	membranes					90:98	supported bilayer membranes	72:98	supported bilayer membranes	72:98	Role of the interactions of soft hyaluronan nanomaterials with CD44 and supported bilayer membranes in the cellular uptake.
34146785	0	41	with	interactions	12:23	arg1	CD44					63:66	CD44	63:66	CD44	63:66	Role of the interactions of soft hyaluronan nanomaterials with CD44 and supported bilayer membranes in the cellular uptake.
34146785	2	42	theme	typical	365:371	arg1	ratio					420:424	high aspect ratio	408:424	high aspect ratio (Γ≈12 to 20)	408:437	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	2	42	theme	typical	365:371	arg1	shape					383:387	a typical hexagonal shape	363:387	a typical hexagonal shape	363:387	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	2	42	theme	typical	365:371	arg1	surfaces					395:402	flat surfaces	390:402	flat surfaces	390:402	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	0	43	theme	nanomaterials	44:56	arg1	interactions					12:23	the interactions	8:23	the interactions of soft hyaluronan nanomaterials with CD44 and supported bilayer membranes	8:98	Role of the interactions of soft hyaluronan nanomaterials with CD44 and supported bilayer membranes in the cellular uptake.
34146785	2	44	dep	20	435:436	arg1	to					432:433	to	432:433	to	432:433	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	3	45	theme	cell	584:587	arg1	line					589:592	a T24 tumor cell line	572:592	a T24 tumor cell line	572:592	Fluorescence imaging revealed that internalization of HA-NPs by a T24 tumor cell line and by macrophages was higher than native polysaccharide in a dose-dependent and time-dependent manners.
34146785	5	46	theme	multivalent	987:997	arg1	interactions					999:1010	multivalent interactions	987:1010	multivalent interactions	987:1010	The results were discussed regarding physical properties of the NPs and the implication of multivalent interactions in HA binding to CD44.
34146785	0	47	theme	hyaluronan	33:42	arg1	nanomaterials					44:56	soft hyaluronan nanomaterials	28:56	soft hyaluronan nanomaterials	28:56	Role of the interactions of soft hyaluronan nanomaterials with CD44 and supported bilayer membranes in the cellular uptake.
34146785	4	48	theme	native	885:890	arg1	HA					892:893	native HA	885:893	native HA	885:893	The ability of HA-NPs to efficiently compete with native HA assessed using Bio-layer interferometry showed that NPs had a stronger interaction with recombinant CD44 receptor compared to native HA.
34146785	4	49	theme	Bio-layer	774:782	arg1	interferometry					784:797	Bio-layer interferometry	774:797	Bio-layer interferometry	774:797	The ability of HA-NPs to efficiently compete with native HA assessed using Bio-layer interferometry showed that NPs had a stronger interaction with recombinant CD44 receptor compared to native HA.
34146785	7	50	theme	receptor-mediated	1288:1304	arg1	interactions					1318:1329	receptor-mediated multivalent interactions	1288:1329	receptor-mediated multivalent interactions	1288:1329	Our findings provide insights into intracellular drug delivery using soft HA-NPs through receptor-mediated multivalent interactions.
34146785	3	51	theme	Fluorescence	508:519	arg1	imaging					521:527	Fluorescence imaging	508:527	Fluorescence imaging	508:527	Fluorescence imaging revealed that internalization of HA-NPs by a T24 tumor cell line and by macrophages was higher than native polysaccharide in a dose-dependent and time-dependent manners.
34146785	5	52	theme	HA	1015:1016	arg1	binding					1018:1024	HA binding	1015:1024	HA binding to CD44	1015:1032	The results were discussed regarding physical properties of the NPs and the implication of multivalent interactions in HA binding to CD44.
34146785	2	53	theme	acid	320:323	arg1	nanoplatelets					330:342	soft hyaluronic acid (HA) nanoplatelets	304:342	soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions	304:505	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	2	53	theme	acid	320:323	arg1	NPs					345:347	NPs	345:347	NPs	345:347	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	4	54	contain	had	815:817	arg1	NPs					811:813	NPs	811:813	NPs	811:813	The ability of HA-NPs to efficiently compete with native HA assessed using Bio-layer interferometry showed that NPs had a stronger interaction with recombinant CD44 receptor compared to native HA.
34146785	4	54	contain	had	815:817	arg2	interaction					830:840	a stronger interaction	819:840	a stronger interaction with recombinant CD44 receptor	819:871	The ability of HA-NPs to efficiently compete with native HA assessed using Bio-layer interferometry showed that NPs had a stronger interaction with recombinant CD44 receptor compared to native HA.
34146785	1	55	theme	ligand	209:214	arg1	ability					196:202	the ability	192:202	the ability of a ligand to specifically bind to targeted cells	192:253	Increasing valence by acting on nanomaterial morphology can enhance the ability of a ligand to specifically bind to targeted cells.
34146785	3	56	theme	T24	574:576	arg1	line					589:592	a T24 tumor cell line	572:592	a T24 tumor cell line	572:592	Fluorescence imaging revealed that internalization of HA-NPs by a T24 tumor cell line and by macrophages was higher than native polysaccharide in a dose-dependent and time-dependent manners.
34146785	2	57	theme	hyaluronic	309:318	arg1	HA					326:327	HA	326:327	HA	326:327	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	2	57	theme	hyaluronic	309:318	arg1	acid					320:323	hyaluronic acid	309:323	soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions	304:505	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	6	58	theme	different	1093:1101	arg1	compositions					1103:1114	different compositions	1093:1114	different compositions	1093:1114	Experiments conducted on supported bilayer membranes with different compositions showed that non-specific interactions of NPs with lipid membranes were negligible.
34146785	5	59	from	binding	1018:1024	arg1	properties					942:951	physical properties	933:951	physical properties of the NPs and the implication of multivalent interactions in HA binding to CD44	933:1032	The results were discussed regarding physical properties of the NPs and the implication of multivalent interactions in HA binding to CD44.
34146785	6	60	theme	bilayer	1070:1076	arg1	membranes					1078:1086	supported bilayer membranes	1060:1086	supported bilayer membranes with different compositions	1060:1114	Experiments conducted on supported bilayer membranes with different compositions showed that non-specific interactions of NPs with lipid membranes were negligible.
34146785	3	61	theme	HA-NPs	562:567	arg1	internalization					543:557	internalization	543:557	internalization of HA-NPs by a T24 tumor cell line and by macrophages	543:611	Fluorescence imaging revealed that internalization of HA-NPs by a T24 tumor cell line and by macrophages was higher than native polysaccharide in a dose-dependent and time-dependent manners.
34146785	4	62	with	interaction	830:840	arg1	receptor					864:871	recombinant CD44 receptor	847:871	recombinant CD44 receptor	847:871	The ability of HA-NPs to efficiently compete with native HA assessed using Bio-layer interferometry showed that NPs had a stronger interaction with recombinant CD44 receptor compared to native HA.
34146785	4	63	theme	native	749:754	arg1	HA					756:757	native HA	749:757	native HA assessed using Bio-layer interferometry	749:797	The ability of HA-NPs to efficiently compete with native HA assessed using Bio-layer interferometry showed that NPs had a stronger interaction with recombinant CD44 receptor compared to native HA.
34146785	7	64	theme	soft	1268:1271	arg1	HA-NPs					1273:1278	soft HA-NPs	1268:1278	soft HA-NPs	1268:1278	Our findings provide insights into intracellular drug delivery using soft HA-NPs through receptor-mediated multivalent interactions.
34146785	2	65	from	microscopy	473:482	arg1	conditions					496:505	hydrated conditions	487:505	hydrated conditions	487:505	Herein, we investigated cell internalization of soft hyaluronic acid (HA) nanoplatelets (NPs) that exhibit a typical hexagonal shape, flat surfaces and high aspect ratio (Γ≈12 to 20), as characterized by atomic force microscopy in hydrated conditions.
34146785	5	66	from	properties	942:951	arg1	binding					1018:1024	HA binding	1015:1024	HA binding to CD44	1015:1032	The results were discussed regarding physical properties of the NPs and the implication of multivalent interactions in HA binding to CD44.
32326580	2	0	theme	pomace	720:725	arg1	powders					727:733	pomace powders	720:733	pomace powders of all polyphenols	720:752	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	7	1	from	increase	1941:1948	arg1	contents					1966:1973	the flavanol contents	1953:1973	the flavanol contents	1953:1973	In this case, the release of (-)-epicatechin was observed, causing an increase in the flavanol contents.
32326580	4	2	from	matter	1312:1317	arg1	tests					1393:1397	the ABTS and FRAP tests	1375:1397	tests	1393:1397	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	0	3	theme	Food	154:157	arg1	Ingredients					159:169	Functional Food Ingredients	143:169	Functional Food Ingredients	143:169	The Impact of Maltodextrin and Inulin on the Protection of Natural Antioxidants in Powders Made of Saskatoon Berry Fruit, Juice, and Pomace as Functional Food Ingredients.
32326580	1	4	theme	radical	392:398	arg1	ABTS					421:424	ABTS	421:424	ABTS	421:424	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	1	4	theme	radical	392:398	arg1	potential					456:464	ferric reducing antioxidant potential	428:464	ferric reducing antioxidant potential (FRAP)	428:471	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	1	4	theme	radical	392:398	arg1	capacity					411:418	radical scavenging capacity	392:418	radical scavenging capacity (ABTS)	392:425	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	5	5	from	decrease	1468:1475	arg1	content					1496:1502	the content	1492:1502	the content of polyphenols	1492:1517	The increase in carrier concentration by 20% caused a decrease of 1.5-fold in the content of polyphenols and a 1.6-fold and 1.5-fold in the antioxidant potential, regardless of the matrix tested.
32326580	5	5	from	decrease	1468:1475	arg1	potential					1566:1574	the antioxidant potential	1550:1574	the antioxidant potential	1550:1574	The increase in carrier concentration by 20% caused a decrease of 1.5-fold in the content of polyphenols and a 1.6-fold and 1.5-fold in the antioxidant potential, regardless of the matrix tested.
32326580	6	6	theme	pomace	1823:1828	arg1	powders					1830:1836	pomace powders	1823:1836	pomace powders	1823:1836	The principal component analysis (PCA) analysis indicated that the freeze-drying process led to the lowest degradation of the identified compounds, regardless of the matrix tested, with the exception of juice and pomace powders dried by vacuum drying at 60 °C.
32326580	4	7	theme	ABTS	1222:1225	arg1	assay					1236:1240	ABTS and FRAP assay	1222:1240	assay	1236:1240	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	6	8	theme	juice	1813:1817	arg1	exception					1800:1808	the exception	1796:1808	the exception of juice and pomace powders dried by vacuum drying at 60 °C	1796:1868	The principal component analysis (PCA) analysis indicated that the freeze-drying process led to the lowest degradation of the identified compounds, regardless of the matrix tested, with the exception of juice and pomace powders dried by vacuum drying at 60 °C.
32326580	0	9	theme	Saskatoon	99:107	arg1	Fruit					115:119	Saskatoon Berry Fruit	99:119	Saskatoon Berry Fruit	99:119	The Impact of Maltodextrin and Inulin on the Protection of Natural Antioxidants in Powders Made of Saskatoon Berry Fruit, Juice, and Pomace as Functional Food Ingredients.
32326580	4	10	theme	FRAP	1231:1234	arg1	assay					1236:1240	ABTS and FRAP assay	1222:1240	assay	1236:1240	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	5	11	from	1.6-fold	1525:1532	arg1	content					1496:1502	the content	1492:1502	the content of polyphenols	1492:1517	The increase in carrier concentration by 20% caused a decrease of 1.5-fold in the content of polyphenols and a 1.6-fold and 1.5-fold in the antioxidant potential, regardless of the matrix tested.
32326580	5	11	from	1.6-fold	1525:1532	arg1	potential					1566:1574	the antioxidant potential	1550:1574	the antioxidant potential	1550:1574	The increase in carrier concentration by 20% caused a decrease of 1.5-fold in the content of polyphenols and a 1.6-fold and 1.5-fold in the antioxidant potential, regardless of the matrix tested.
32326580	2	12	theme	phenolic	834:841	arg1	acids					843:847	phenolic acids	834:847	phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder)	834:919	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	13	theme	juice	965:969	arg1	powder					971:976	juice powder	965:976	juice powder	965:976	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	5	14	theme	carrier	1430:1436	arg1	concentration					1438:1450	carrier concentration	1430:1450	carrier concentration	1430:1450	The increase in carrier concentration by 20% caused a decrease of 1.5-fold in the content of polyphenols and a 1.6-fold and 1.5-fold in the antioxidant potential, regardless of the matrix tested.
32326580	9	15	theme	mentioned	2191:2199	arg1	carriers					2201:2208	the mentioned carriers	2187:2208	the mentioned carriers	2187:2208	Properly designed high-quality Saskatoon berry powders with the mentioned carriers may be used as nutraceutical additives to fortify food products and to improve their functional properties.
32326580	2	16	theme	liquid	518:523	arg1	UPLC-PDA-ESI-MS/MS					541:558	UPLC-PDA-ESI-MS/MS	541:558	UPLC-PDA-ESI-MS/MS	541:558	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	16	theme	liquid	518:523	arg1	chromatography					525:538	Ultra-high performance liquid chromatography	495:538	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS)	495:559	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	0	17	from	Protection	45:54	arg1	Powders					83:89	Powders	83:89	Powders Made of Saskatoon Berry Fruit, Juice, and Pomace as Functional Food Ingredients	83:169	The Impact of Maltodextrin and Inulin on the Protection of Natural Antioxidants in Powders Made of Saskatoon Berry Fruit, Juice, and Pomace as Functional Food Ingredients.
32326580	1	18	theme	bioactive	346:354	arg1	compounds					356:364	bioactive compounds	346:364	bioactive compounds	346:364	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	2	19	theme	Ultra-high	495:504	arg1	UPLC-PDA-ESI-MS/MS					541:558	UPLC-PDA-ESI-MS/MS	541:558	UPLC-PDA-ESI-MS/MS	541:558	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	19	theme	Ultra-high	495:504	arg1	chromatography					525:538	Ultra-high performance liquid chromatography	495:538	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS)	495:559	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	1	20	dep	capacity	382:389	arg1	ABTS					421:424	ABTS	421:424	ABTS	421:424	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	1	20	dep	capacity	382:389	arg1	potential					456:464	ferric reducing antioxidant potential	428:464	ferric reducing antioxidant potential (FRAP)	428:471	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	1	20	dep	capacity	382:389	arg1	capacity					411:418	radical scavenging capacity	392:418	radical scavenging capacity (ABTS)	392:425	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	3	21	theme	polyphenols	1033:1043	arg1	content					1022:1028	the content	1018:1028	the content of polyphenols	1018:1043	In general, the content of polyphenols was more dependent on the content than on the type of carrier used for drying, regardless of the matrix tested.
32326580	1	22	theme	Saskatoon	285:293	arg1	fruit					301:305	Saskatoon berry fruit	285:305	Saskatoon berry fruit	285:305	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	9	23	used	used	2217:2220	arg2	additives					2239:2247	nutraceutical additives	2225:2247	nutraceutical additives	2225:2247	Properly designed high-quality Saskatoon berry powders with the mentioned carriers may be used as nutraceutical additives to fortify food products and to improve their functional properties.
32326580	9	23	used	used	2217:2220	arg2	powders					2174:2180	Properly designed high-quality Saskatoon berry powders	2127:2180	Properly designed high-quality Saskatoon berry powders with the mentioned carriers	2127:2208	Properly designed high-quality Saskatoon berry powders with the mentioned carriers may be used as nutraceutical additives to fortify food products and to improve their functional properties.
32326580	6	24	theme	vacuum	1847:1852	arg1	drying					1854:1859	vacuum drying	1847:1859	vacuum drying at 60 °C	1847:1868	The principal component analysis (PCA) analysis indicated that the freeze-drying process led to the lowest degradation of the identified compounds, regardless of the matrix tested, with the exception of juice and pomace powders dried by vacuum drying at 60 °C.
32326580	0	25	theme	Natural	59:65	arg1	Antioxidants					67:78	Natural Antioxidants	59:78	Natural Antioxidants	59:78	The Impact of Maltodextrin and Inulin on the Protection of Natural Antioxidants in Powders Made of Saskatoon Berry Fruit, Juice, and Pomace as Functional Food Ingredients.
32326580	1	26	theme	fruit	301:305	arg1	drying					275:280	vacuum drying	268:280	vacuum drying of Saskatoon berry fruit, juice, and pomace	268:324	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	2	27	dep	anthocyanins	756:767	arg1	%					819:819	34%	817:819	34%	817:819	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	27	dep	anthocyanins	756:767	arg1	%					772:772	26%	770:772	26% for fruit powder	770:789	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	27	dep	anthocyanins	756:767	arg1	%					793:793	5%	792:793	5% for juice powder	792:810	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	28	theme	juice	799:803	arg1	powder					805:810	juice powder	799:810	juice powder	799:810	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	29	dep	acids	843:847	arg1	%					900:900	20%	898:900	20%	898:900	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	29	dep	acids	843:847	arg1	%					852:852	33%	850:852	33% for fruit powder	850:869	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	29	dep	acids	843:847	arg1	%					874:874	55%	872:874	55% for juice powder	872:891	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	1	30	theme	juice	308:312	arg1	drying					275:280	vacuum drying	268:280	vacuum drying of Saskatoon berry fruit, juice, and pomace	268:324	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	9	31	theme	food	2260:2263	arg1	products					2265:2272	food products	2260:2272	food products	2260:2272	Properly designed high-quality Saskatoon berry powders with the mentioned carriers may be used as nutraceutical additives to fortify food products and to improve their functional properties.
32326580	4	32	theme	g	1306:1306	arg1	d.m.					1320:1323	d.m.	1320:1323	d.m.	1320:1323	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	4	32	theme	g	1306:1306	arg1	matter					1312:1317	5054.2 mg/100 g dry matter	1292:1317	5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m.	1292:1370	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	4	32	theme	g	1306:1306	arg1	sum					1169:1171	The average sum	1157:1171	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition	1157:1285	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	2	33	theme	major	582:586	arg1	acids					843:847	phenolic acids	834:847	phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder)	834:919	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	33	theme	major	582:586	arg1	groups					588:593	major groups	582:593	major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder)	582:1003	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	33	theme	major	582:586	arg1	flavan-3-ols					631:642	flavan-3-ols	631:642	flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols)	631:753	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	33	theme	major	582:586	arg1	compounds					611:619	polyphenolic compounds	598:619	polyphenolic compounds	598:619	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	33	theme	major	582:586	arg1	flavanols					926:934	flavanols	926:934	flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder)	926:1003	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	33	theme	major	582:586	arg1	anthocyanins					756:767	anthocyanins	756:767	anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace)	756:831	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	6	34	theme	compounds	1747:1755	arg1	degradation					1717:1727	the lowest degradation	1706:1727	the lowest degradation of the identified compounds	1706:1755	The principal component analysis (PCA) analysis indicated that the freeze-drying process led to the lowest degradation of the identified compounds, regardless of the matrix tested, with the exception of juice and pomace powders dried by vacuum drying at 60 °C.
32326580	8	35	theme	composition	2041:2051	arg1	effect					2009:2014	the effect	2005:2014	the effect of processing and matrix composition on the preservation of antioxidant bioactives in Saskatoon berry powders	2005:2124	Thus, this work demonstrated the effect of processing and matrix composition on the preservation of antioxidant bioactives in Saskatoon berry powders.
32326580	1	36	theme	inulin	229:234	arg1	effect					219:224	the effect	215:224	the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained	215:492	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	2	37	dep	flavan-3-ols	631:642	arg1	%					647:647	35%	645:647	35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols	645:752	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	37	dep	flavan-3-ols	631:642	arg1	polyphenols					656:666	all polyphenols	652:666	all polyphenols	652:666	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	38	theme	fruit	778:782	arg1	powder					784:789	fruit powder	778:789	fruit powder	778:789	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	4	39	theme	mmol	1349:1352	arg1	d.m.					1367:1370	d.m.	1367:1370	d.m.	1367:1370	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	4	39	theme	mmol	1349:1352	arg1	g					1365:1365	5.3 and 3.6 mmol Trolox/100 g	1337:1365	5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m.	1292:1370	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	2	40	theme	compounds	611:619	arg1	acids					843:847	phenolic acids	834:847	phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder)	834:919	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	40	theme	compounds	611:619	arg1	groups					588:593	major groups	582:593	major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder)	582:1003	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	40	theme	compounds	611:619	arg1	flavan-3-ols					631:642	flavan-3-ols	631:642	flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols)	631:753	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	40	theme	compounds	611:619	arg1	compounds					611:619	polyphenolic compounds	598:619	polyphenolic compounds	598:619	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	40	theme	compounds	611:619	arg1	flavanols					926:934	flavanols	926:934	flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder)	926:1003	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	40	theme	compounds	611:619	arg1	anthocyanins					756:767	anthocyanins	756:767	anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace)	756:831	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	41	theme	pomace	906:911	arg1	powder					913:918	pomace powder	906:918	pomace powder	906:918	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	6	42	theme	analysis	1634:1641	arg1	analysis					1649:1656	The principal component analysis (PCA) analysis	1610:1656	The principal component analysis (PCA) analysis	1610:1656	The principal component analysis (PCA) analysis indicated that the freeze-drying process led to the lowest degradation of the identified compounds, regardless of the matrix tested, with the exception of juice and pomace powders dried by vacuum drying at 60 °C.
32326580	6	42	theme	analysis	1634:1641	arg1	PCA					1644:1646	PCA	1644:1646	PCA	1644:1646	The principal component analysis (PCA) analysis indicated that the freeze-drying process led to the lowest degradation of the identified compounds, regardless of the matrix tested, with the exception of juice and pomace powders dried by vacuum drying at 60 °C.
32326580	1	43	theme	maltodextrin	240:251	arg1	effect					219:224	the effect	215:224	the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained	215:492	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	9	44	theme	functional	2295:2304	arg1	properties					2306:2315	their functional properties	2289:2315	their functional properties	2289:2315	Properly designed high-quality Saskatoon berry powders with the mentioned carriers may be used as nutraceutical additives to fortify food products and to improve their functional properties.
32326580	2	45	theme	fruit	944:948	arg1	powder					950:955	fruit powder	944:955	fruit powder	944:955	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	3	46	theme	carrier	1099:1105	arg1	type					1091:1094	the type	1087:1094	the type of carrier used for drying	1087:1121	In general, the content of polyphenols was more dependent on the content than on the type of carrier used for drying, regardless of the matrix tested.
32326580	6	47	theme	lowest	1710:1715	arg1	degradation					1717:1727	the lowest degradation	1706:1727	the lowest degradation of the identified compounds	1706:1755	The principal component analysis (PCA) analysis indicated that the freeze-drying process led to the lowest degradation of the identified compounds, regardless of the matrix tested, with the exception of juice and pomace powders dried by vacuum drying at 60 °C.
32326580	9	48	theme	designed	2136:2143	arg1	additives					2239:2247	nutraceutical additives	2225:2247	nutraceutical additives	2225:2247	Properly designed high-quality Saskatoon berry powders with the mentioned carriers may be used as nutraceutical additives to fortify food products and to improve their functional properties.
32326580	9	48	theme	designed	2136:2143	arg1	powders					2174:2180	Properly designed high-quality Saskatoon berry powders	2127:2180	Properly designed high-quality Saskatoon berry powders with the mentioned carriers	2127:2208	Properly designed high-quality Saskatoon berry powders with the mentioned carriers may be used as nutraceutical additives to fortify food products and to improve their functional properties.
32326580	6	49	theme	principal	1614:1622	arg1	analysis					1634:1641	The principal component analysis	1610:1641	The principal component analysis (PCA) analysis	1610:1656	The principal component analysis (PCA) analysis indicated that the freeze-drying process led to the lowest degradation of the identified compounds, regardless of the matrix tested, with the exception of juice and pomace powders dried by vacuum drying at 60 °C.
32326580	1	50	theme	ferric	428:433	arg1	FRAP					467:470	FRAP	467:470	FRAP	467:470	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	1	50	theme	ferric	428:433	arg1	capacity					411:418	radical scavenging capacity	392:418	radical scavenging capacity (ABTS)	392:425	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	1	50	theme	ferric	428:433	arg1	potential					456:464	ferric reducing antioxidant potential	428:464	ferric reducing antioxidant potential (FRAP)	428:471	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	4	51	theme	activity	1208:1215	arg1	matter					1312:1317	5054.2 mg/100 g dry matter	1292:1317	5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m.	1292:1370	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	4	51	theme	activity	1208:1215	arg1	sum					1169:1171	The average sum	1157:1171	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition	1157:1285	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	1	52	theme	antioxidant	444:454	arg1	FRAP					467:470	FRAP	467:470	FRAP	467:470	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	1	52	theme	antioxidant	444:454	arg1	capacity					411:418	radical scavenging capacity	392:418	radical scavenging capacity (ABTS)	392:425	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	1	52	theme	antioxidant	444:454	arg1	potential					456:464	ferric reducing antioxidant potential	428:464	ferric reducing antioxidant potential (FRAP)	428:471	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	2	53	theme	polyphenols	742:752	arg1	powders					727:733	pomace powders	720:733	pomace powders of all polyphenols	720:752	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	4	54	theme	FRAP	1388:1391	arg1	tests					1393:1397	the ABTS and FRAP tests	1375:1397	tests	1393:1397	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	7	55	dep	-epicatechin	1903:1914	arg1	-					1901:1901	-	1901:1901	-	1901:1901	In this case, the release of (-)-epicatechin was observed, causing an increase in the flavanol contents.
32326580	9	56	theme	nutraceutical	2225:2237	arg1	additives					2239:2247	nutraceutical additives	2225:2247	nutraceutical additives	2225:2247	Properly designed high-quality Saskatoon berry powders with the mentioned carriers may be used as nutraceutical additives to fortify food products and to improve their functional properties.
32326580	9	56	theme	nutraceutical	2225:2237	arg1	powders					2174:2180	Properly designed high-quality Saskatoon berry powders	2127:2180	Properly designed high-quality Saskatoon berry powders with the mentioned carriers	2127:2208	Properly designed high-quality Saskatoon berry powders with the mentioned carriers may be used as nutraceutical additives to fortify food products and to improve their functional properties.
32326580	4	57	theme	polyphenols	1176:1186	arg1	matter					1312:1317	5054.2 mg/100 g dry matter	1292:1317	5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m.	1292:1370	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	4	57	theme	polyphenols	1176:1186	arg1	sum					1169:1171	The average sum	1157:1171	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition	1157:1285	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	9	58	theme	high-quality	2145:2156	arg1	additives					2239:2247	nutraceutical additives	2225:2247	nutraceutical additives	2225:2247	Properly designed high-quality Saskatoon berry powders with the mentioned carriers may be used as nutraceutical additives to fortify food products and to improve their functional properties.
32326580	9	58	theme	high-quality	2145:2156	arg1	powders					2174:2180	Properly designed high-quality Saskatoon berry powders	2127:2180	Properly designed high-quality Saskatoon berry powders with the mentioned carriers	2127:2208	Properly designed high-quality Saskatoon berry powders with the mentioned carriers may be used as nutraceutical additives to fortify food products and to improve their functional properties.
32326580	0	59	theme	Functional	143:152	arg1	Ingredients					159:169	Functional Food Ingredients	143:169	Functional Food Ingredients	143:169	The Impact of Maltodextrin and Inulin on the Protection of Natural Antioxidants in Powders Made of Saskatoon Berry Fruit, Juice, and Pomace as Functional Food Ingredients.
32326580	7	60	theme	-epicatechin	1903:1914	arg1	release					1889:1895	the release	1885:1895	the release of (-)-epicatechin	1885:1914	In this case, the release of (-)-epicatechin was observed, causing an increase in the flavanol contents.
32326580	8	61	theme	bioactives	2088:2097	arg1	preservation					2060:2071	the preservation	2056:2071	the preservation of antioxidant bioactives in Saskatoon berry powders	2056:2124	Thus, this work demonstrated the effect of processing and matrix composition on the preservation of antioxidant bioactives in Saskatoon berry powders.
32326580	4	62	theme	ABTS	1379:1382	arg1	tests					1393:1397	the ABTS and FRAP tests	1375:1397	tests	1393:1397	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	1	63	theme	scavenging	400:409	arg1	ABTS					421:424	ABTS	421:424	ABTS	421:424	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	1	63	theme	scavenging	400:409	arg1	potential					456:464	ferric reducing antioxidant potential	428:464	ferric reducing antioxidant potential (FRAP)	428:471	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	1	63	theme	scavenging	400:409	arg1	capacity					411:418	radical scavenging capacity	392:418	radical scavenging capacity (ABTS)	392:425	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	4	64	theme	powders	1250:1256	arg1	matter					1312:1317	5054.2 mg/100 g dry matter	1292:1317	5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m.	1292:1370	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	4	64	theme	powders	1250:1256	arg1	sum					1169:1171	The average sum	1157:1171	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition	1157:1285	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	6	65	theme	identified	1736:1745	arg1	compounds					1747:1755	the identified compounds	1732:1755	the identified compounds	1732:1755	The principal component analysis (PCA) analysis indicated that the freeze-drying process led to the lowest degradation of the identified compounds, regardless of the matrix tested, with the exception of juice and pomace powders dried by vacuum drying at 60 °C.
32326580	2	66	theme	fruit	672:676	arg1	powder					678:683	fruit powder	672:683	fruit powder	672:683	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	5	67	theme	polyphenols	1507:1517	arg1	content					1496:1502	the content	1492:1502	the content of polyphenols	1492:1517	The increase in carrier concentration by 20% caused a decrease of 1.5-fold in the content of polyphenols and a 1.6-fold and 1.5-fold in the antioxidant potential, regardless of the matrix tested.
32326580	9	68	theme	Saskatoon	2158:2166	arg1	additives					2239:2247	nutraceutical additives	2225:2247	nutraceutical additives	2225:2247	Properly designed high-quality Saskatoon berry powders with the mentioned carriers may be used as nutraceutical additives to fortify food products and to improve their functional properties.
32326580	9	68	theme	Saskatoon	2158:2166	arg1	powders					2174:2180	Properly designed high-quality Saskatoon berry powders	2127:2180	Properly designed high-quality Saskatoon berry powders with the mentioned carriers	2127:2208	Properly designed high-quality Saskatoon berry powders with the mentioned carriers may be used as nutraceutical additives to fortify food products and to improve their functional properties.
32326580	5	69	dep	1.6-fold	1525:1532	arg1	a					1523:1523	a	1523:1523	a	1523:1523	The increase in carrier concentration by 20% caused a decrease of 1.5-fold in the content of polyphenols and a 1.6-fold and 1.5-fold in the antioxidant potential, regardless of the matrix tested.
32326580	2	70	theme	juice	694:698	arg1	powder					700:705	juice powder	694:705	juice powder	694:705	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	71	used	used	565:568	arg2	chromatography					525:538	Ultra-high performance liquid chromatography	495:538	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS)	495:559	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	71	used	used	565:568	arg2	UPLC-PDA-ESI-MS/MS					541:558	UPLC-PDA-ESI-MS/MS	541:558	UPLC-PDA-ESI-MS/MS	541:558	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	0	72	theme	Berry	109:113	arg1	Fruit					115:119	Saskatoon Berry Fruit	99:119	Saskatoon Berry Fruit	99:119	The Impact of Maltodextrin and Inulin on the Protection of Natural Antioxidants in Powders Made of Saskatoon Berry Fruit, Juice, and Pomace as Functional Food Ingredients.
32326580	8	73	from	effect	2009:2014	arg1	preservation					2060:2071	the preservation	2056:2071	the preservation of antioxidant bioactives in Saskatoon berry powders	2056:2124	Thus, this work demonstrated the effect of processing and matrix composition on the preservation of antioxidant bioactives in Saskatoon berry powders.
32326580	8	74	theme	berry	2112:2116	arg1	powders					2118:2124	Saskatoon berry powders	2102:2124	Saskatoon berry powders	2102:2124	Thus, this work demonstrated the effect of processing and matrix composition on the preservation of antioxidant bioactives in Saskatoon berry powders.
32326580	4	75	theme	addition	1278:1285	arg1	%					1265:1265	30%	1263:1265	30% of carrier addition	1263:1285	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	4	75	theme	addition	1278:1285	arg1	addition					1278:1285	carrier addition	1270:1285	carrier addition	1270:1285	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	9	76	theme	berry	2168:2172	arg1	additives					2239:2247	nutraceutical additives	2225:2247	nutraceutical additives	2225:2247	Properly designed high-quality Saskatoon berry powders with the mentioned carriers may be used as nutraceutical additives to fortify food products and to improve their functional properties.
32326580	9	76	theme	berry	2168:2172	arg1	powders					2174:2180	Properly designed high-quality Saskatoon berry powders	2127:2180	Properly designed high-quality Saskatoon berry powders with the mentioned carriers	2127:2208	Properly designed high-quality Saskatoon berry powders with the mentioned carriers may be used as nutraceutical additives to fortify food products and to improve their functional properties.
32326580	6	77	from	°C	1867:1868	arg1	drying					1854:1859	vacuum drying	1847:1859	vacuum drying at 60 °C	1847:1868	The principal component analysis (PCA) analysis indicated that the freeze-drying process led to the lowest degradation of the identified compounds, regardless of the matrix tested, with the exception of juice and pomace powders dried by vacuum drying at 60 °C.
32326580	2	78	theme	pomace	990:995	arg1	powder					997:1002	pomace powder	990:1002	pomace powder	990:1002	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	79	theme	performance	506:516	arg1	UPLC-PDA-ESI-MS/MS					541:558	UPLC-PDA-ESI-MS/MS	541:558	UPLC-PDA-ESI-MS/MS	541:558	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	79	theme	performance	506:516	arg1	chromatography					525:538	Ultra-high performance liquid chromatography	495:538	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS)	495:559	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	5	80	theme	antioxidant	1554:1564	arg1	potential					1566:1574	the antioxidant potential	1550:1574	the antioxidant potential	1550:1574	The increase in carrier concentration by 20% caused a decrease of 1.5-fold in the content of polyphenols and a 1.6-fold and 1.5-fold in the antioxidant potential, regardless of the matrix tested.
32326580	1	81	theme	compounds	356:364	arg1	retention					333:341	the retention	329:341	the retention of bioactive compounds	329:364	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	1	81	theme	compounds	356:364	arg1	capacity					382:389	antioxidant capacity	370:389	antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained	370:492	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	8	82	theme	Saskatoon	2102:2110	arg1	powders					2118:2124	Saskatoon berry powders	2102:2124	Saskatoon berry powders	2102:2124	Thus, this work demonstrated the effect of processing and matrix composition on the preservation of antioxidant bioactives in Saskatoon berry powders.
32326580	2	83	theme	polyphenols	656:666	arg1	%					647:647	35%	645:647	35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols	645:752	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	83	theme	polyphenols	656:666	arg1	polyphenols					656:666	all polyphenols	652:666	all polyphenols	652:666	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	1	84	theme	antioxidant	370:380	arg1	capacity					382:389	antioxidant capacity	370:389	antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained	370:492	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	7	85	located	observed	1920:1927	arg2	release					1889:1895	the release	1885:1895	the release of (-)-epicatechin	1885:1914	In this case, the release of (-)-epicatechin was observed, causing an increase in the flavanol contents.
32326580	7	85	located	observed	1920:1927	arg1	case					1879:1882	this case	1874:1882	this case	1874:1882	In this case, the release of (-)-epicatechin was observed, causing an increase in the flavanol contents.
32326580	8	86	from	preservation	2060:2071	arg1	powders					2118:2124	Saskatoon berry powders	2102:2124	Saskatoon berry powders	2102:2124	Thus, this work demonstrated the effect of processing and matrix composition on the preservation of antioxidant bioactives in Saskatoon berry powders.
32326580	0	87	theme	Antioxidants	67:78	arg1	Protection					45:54	the Protection	41:54	the Protection of Natural Antioxidants in Powders Made of Saskatoon Berry Fruit, Juice, and Pomace as Functional Food Ingredients	41:169	The Impact of Maltodextrin and Inulin on the Protection of Natural Antioxidants in Powders Made of Saskatoon Berry Fruit, Juice, and Pomace as Functional Food Ingredients.
32326580	1	88	theme	berry	295:299	arg1	fruit					301:305	Saskatoon berry fruit	285:305	Saskatoon berry fruit	285:305	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	6	89	theme	freeze-drying	1677:1689	arg1	process					1691:1697	the freeze-drying process	1673:1697	the freeze-drying process	1673:1697	The principal component analysis (PCA) analysis indicated that the freeze-drying process led to the lowest degradation of the identified compounds, regardless of the matrix tested, with the exception of juice and pomace powders dried by vacuum drying at 60 °C.
32326580	0	90	from	Impact	4:9	arg1	Protection					45:54	the Protection	41:54	the Protection of Natural Antioxidants in Powders Made of Saskatoon Berry Fruit, Juice, and Pomace as Functional Food Ingredients	41:169	The Impact of Maltodextrin and Inulin on the Protection of Natural Antioxidants in Powders Made of Saskatoon Berry Fruit, Juice, and Pomace as Functional Food Ingredients.
32326580	2	91	theme	polyphenolic	598:609	arg1	flavanols					926:934	flavanols	926:934	flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder)	926:1003	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	91	theme	polyphenolic	598:609	arg1	anthocyanins					756:767	anthocyanins	756:767	anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace)	756:831	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	91	theme	polyphenolic	598:609	arg1	acids					843:847	phenolic acids	834:847	phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder)	834:919	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	91	theme	polyphenolic	598:609	arg1	flavan-3-ols					631:642	flavan-3-ols	631:642	flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols)	631:753	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	91	theme	polyphenolic	598:609	arg1	compounds					611:619	polyphenolic compounds	598:619	polyphenolic compounds	598:619	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	8	92	theme	processing	2019:2028	arg1	effect					2009:2014	the effect	2005:2014	the effect of processing and matrix composition on the preservation of antioxidant bioactives in Saskatoon berry powders	2005:2124	Thus, this work demonstrated the effect of processing and matrix composition on the preservation of antioxidant bioactives in Saskatoon berry powders.
32326580	5	93	from	1.5-fold	1538:1545	arg1	content					1496:1502	the content	1492:1502	the content of polyphenols	1492:1517	The increase in carrier concentration by 20% caused a decrease of 1.5-fold in the content of polyphenols and a 1.6-fold and 1.5-fold in the antioxidant potential, regardless of the matrix tested.
32326580	5	93	from	1.5-fold	1538:1545	arg1	potential					1566:1574	the antioxidant potential	1550:1574	the antioxidant potential	1550:1574	The increase in carrier concentration by 20% caused a decrease of 1.5-fold in the content of polyphenols and a 1.6-fold and 1.5-fold in the antioxidant potential, regardless of the matrix tested.
32326580	2	94	dep	flavanols	926:934	arg1	%					984:984	7%	983:984	7%	983:984	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	94	dep	flavanols	926:934	arg1	%					938:938	6%	937:938	6% for fruit powder	937:955	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	2	94	dep	flavanols	926:934	arg1	%					959:959	6%	958:959	6% for juice powder	958:976	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	8	95	theme	matrix	2034:2039	arg1	composition					2041:2051	matrix composition	2034:2051	matrix composition	2034:2051	Thus, this work demonstrated the effect of processing and matrix composition on the preservation of antioxidant bioactives in Saskatoon berry powders.
32326580	5	96	from	increase	1418:1425	arg1	concentration					1438:1450	carrier concentration	1430:1450	carrier concentration	1430:1450	The increase in carrier concentration by 20% caused a decrease of 1.5-fold in the content of polyphenols and a 1.6-fold and 1.5-fold in the antioxidant potential, regardless of the matrix tested.
32326580	4	97	theme	mg/100	1299:1304	arg1	d.m.					1320:1323	d.m.	1320:1323	d.m.	1320:1323	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	4	97	theme	mg/100	1299:1304	arg1	matter					1312:1317	5054.2 mg/100 g dry matter	1292:1317	5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m.	1292:1370	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	4	97	theme	mg/100	1299:1304	arg1	sum					1169:1171	The average sum	1157:1171	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition	1157:1285	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	1	98	theme	powders	477:483	arg1	retention					333:341	the retention	329:341	the retention of bioactive compounds	329:364	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	1	98	theme	powders	477:483	arg1	capacity					382:389	antioxidant capacity	370:389	antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained	370:492	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	1	99	theme	pomace	319:324	arg1	drying					275:280	vacuum drying	268:280	vacuum drying of Saskatoon berry fruit, juice, and pomace	268:324	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	6	100	theme	powders	1830:1836	arg1	exception					1800:1808	the exception	1796:1808	the exception of juice and pomace powders dried by vacuum drying at 60 °C	1796:1868	The principal component analysis (PCA) analysis indicated that the freeze-drying process led to the lowest degradation of the identified compounds, regardless of the matrix tested, with the exception of juice and pomace powders dried by vacuum drying at 60 °C.
32326580	4	101	with	powders	1250:1256	arg1	%					1265:1265	30%	1263:1265	30% of carrier addition	1263:1285	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	4	101	with	powders	1250:1256	arg1	addition					1278:1285	carrier addition	1270:1285	carrier addition	1270:1285	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	4	102	theme	dry	1308:1310	arg1	d.m.					1320:1323	d.m.	1320:1323	d.m.	1320:1323	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	4	102	theme	dry	1308:1310	arg1	matter					1312:1317	5054.2 mg/100 g dry matter	1292:1317	5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m.	1292:1370	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	4	102	theme	dry	1308:1310	arg1	sum					1169:1171	The average sum	1157:1171	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition	1157:1285	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	9	103	with	powders	2174:2180	arg1	carriers					2201:2208	the mentioned carriers	2187:2208	the mentioned carriers	2187:2208	Properly designed high-quality Saskatoon berry powders with the mentioned carriers may be used as nutraceutical additives to fortify food products and to improve their functional properties.
32326580	4	104	theme	average	1161:1167	arg1	matter					1312:1317	5054.2 mg/100 g dry matter	1292:1317	5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m.	1292:1370	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	4	104	theme	average	1161:1167	arg1	sum					1169:1171	The average sum	1157:1171	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition	1157:1285	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	0	105	theme	Maltodextrin	14:25	arg1	Impact					4:9	The Impact	0:9	The Impact of Maltodextrin and Inulin on the Protection of Natural Antioxidants in Powders Made of Saskatoon Berry Fruit, Juice, and Pomace as Functional Food Ingredients.	0:170	The Impact of Maltodextrin and Inulin on the Protection of Natural Antioxidants in Powders Made of Saskatoon Berry Fruit, Juice, and Pomace as Functional Food Ingredients.
32326580	7	106	theme	flavanol	1957:1964	arg1	contents					1966:1973	the flavanol contents	1953:1973	the flavanol contents	1953:1973	In this case, the release of (-)-epicatechin was observed, causing an increase in the flavanol contents.
32326580	4	107	from	g	1365:1365	arg1	tests					1393:1397	the ABTS and FRAP tests	1375:1397	tests	1393:1397	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	8	108	theme	antioxidant	2076:2086	arg1	bioactives					2088:2097	antioxidant bioactives	2076:2097	antioxidant bioactives	2076:2097	Thus, this work demonstrated the effect of processing and matrix composition on the preservation of antioxidant bioactives in Saskatoon berry powders.
32326580	0	109	theme	Inulin	31:36	arg1	Impact					4:9	The Impact	0:9	The Impact of Maltodextrin and Inulin on the Protection of Natural Antioxidants in Powders Made of Saskatoon Berry Fruit, Juice, and Pomace as Functional Food Ingredients.	0:170	The Impact of Maltodextrin and Inulin on the Protection of Natural Antioxidants in Powders Made of Saskatoon Berry Fruit, Juice, and Pomace as Functional Food Ingredients.
32326580	4	110	theme	Trolox/100	1354:1363	arg1	d.m.					1367:1370	d.m.	1367:1370	d.m.	1367:1370	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	4	110	theme	Trolox/100	1354:1363	arg1	g					1365:1365	5.3 and 3.6 mmol Trolox/100 g	1337:1365	5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m.	1292:1370	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	5	111	theme	1.5-fold	1480:1487	arg1	1.5-fold					1538:1545	1.5-fold	1538:1545	1.5-fold	1538:1545	The increase in carrier concentration by 20% caused a decrease of 1.5-fold in the content of polyphenols and a 1.6-fold and 1.5-fold in the antioxidant potential, regardless of the matrix tested.
32326580	5	111	theme	1.5-fold	1480:1487	arg1	1.6-fold					1525:1532	1.6-fold	1525:1532	1.6-fold	1525:1532	The increase in carrier concentration by 20% caused a decrease of 1.5-fold in the content of polyphenols and a 1.6-fold and 1.5-fold in the antioxidant potential, regardless of the matrix tested.
32326580	5	111	theme	1.5-fold	1480:1487	arg1	decrease					1468:1475	a decrease	1466:1475	a decrease of 1.5-fold in the content of polyphenols	1466:1517	The increase in carrier concentration by 20% caused a decrease of 1.5-fold in the content of polyphenols and a 1.6-fold and 1.5-fold in the antioxidant potential, regardless of the matrix tested.
32326580	6	112	theme	component	1624:1632	arg1	analysis					1634:1641	The principal component analysis	1610:1641	The principal component analysis (PCA) analysis	1610:1656	The principal component analysis (PCA) analysis indicated that the freeze-drying process led to the lowest degradation of the identified compounds, regardless of the matrix tested, with the exception of juice and pomace powders dried by vacuum drying at 60 °C.
32326580	1	113	theme	vacuum	268:273	arg1	drying					275:280	vacuum drying	268:280	vacuum drying of Saskatoon berry fruit, juice, and pomace	268:324	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	2	114	theme	fruit	858:862	arg1	powder					864:869	fruit powder	858:869	fruit powder	858:869	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	1	115	theme	reducing	435:442	arg1	FRAP					467:470	FRAP	467:470	FRAP	467:470	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	1	115	theme	reducing	435:442	arg1	capacity					411:418	radical scavenging capacity	392:418	radical scavenging capacity (ABTS)	392:425	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	1	115	theme	reducing	435:442	arg1	potential					456:464	ferric reducing antioxidant potential	428:464	ferric reducing antioxidant potential (FRAP)	428:471	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	4	116	theme	antioxidant	1196:1206	arg1	activity					1208:1215	the antioxidant activity	1192:1215	the antioxidant activity (for ABTS and FRAP assay)	1192:1241	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	4	117	theme	carrier	1270:1276	arg1	addition					1278:1285	carrier addition	1270:1285	carrier addition	1270:1285	The average sum of polyphenols and the antioxidant activity (for ABTS and FRAP assay) of the powders with 30% of carrier addition were 5054.2 mg/100 g dry matter (d.m.) as well as 5.3 and 3.6 mmol Trolox/100 g d.m. in the ABTS and FRAP tests, respectively.
32326580	2	118	theme	juice	880:884	arg1	powder					886:891	juice powder	880:891	juice powder	880:891	Ultra-high performance liquid chromatography (UPLC-PDA-ESI-MS/MS) was used to identify major groups of polyphenolic compounds, such as: flavan-3-ols (35% of all polyphenols for fruit powder, 33% for juice powder, and 39% for pomace powders of all polyphenols), anthocyanins (26% for fruit powder, 5% for juice powder, and 34% for pomace), phenolic acids (33% for fruit powder, 55% for juice powder, and 20% for pomace powder), and flavanols (6% for fruit powder, 6% for juice powder, and 7% for pomace powder).
32326580	1	119	theme	study	194:198	arg1	objective					176:184	The objective	172:184	The objective of this study	172:198	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	1	120	from	effect	219:224	arg1	retention					333:341	the retention	329:341	the retention of bioactive compounds	329:364	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
32326580	1	120	from	effect	219:224	arg1	capacity					382:389	antioxidant capacity	370:389	antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained	370:492	The objective of this study was to examine the effect of inulin and maltodextrin applied during vacuum drying of Saskatoon berry fruit, juice, and pomace on the retention of bioactive compounds and antioxidant capacity (radical scavenging capacity (ABTS), ferric reducing antioxidant potential (FRAP)) of powders obtained.
31896257	2	0	theme	wall	413:416	arg1	integrities					418:428	cell wall integrities	408:428	cell wall integrities	408:428	In the present study, we isolated intact cotyledon cells from pinto bean seeds as whole pulse food and RS 1 model and obtained a series of cell wall integrities through controlled enzymolysis.
31896257	3	1	theme	heat-treated	640:651	arg1	cells					664:668	heat-treated pinto bean cells	640:668	heat-treated pinto bean cells	640:668	In vitro human fecal fermentation performance and microbiota responses were tested, and we reported that the cell wall integrity controls the in vitro fecal fermentation rate of heat-treated pinto bean cells.
31896257	3	2	theme	bean	659:662	arg1	cells					664:668	heat-treated pinto bean cells	640:668	heat-treated pinto bean cells	640:668	In vitro human fecal fermentation performance and microbiota responses were tested, and we reported that the cell wall integrity controls the in vitro fecal fermentation rate of heat-treated pinto bean cells.
31896257	1	3	theme	resistant	137:145	arg1	RS					155:156	RS 1	155:158	RS 1	155:158	The physical structure of type 1 resistant starch (RS 1) could influence the metabolite production and stimulate the growth of specific bacteria in the human colon.
31896257	1	3	theme	resistant	137:145	arg1	starch					147:152	resistant starch	137:152	type 1 resistant starch (RS 1)	130:159	The physical structure of type 1 resistant starch (RS 1) could influence the metabolite production and stimulate the growth of specific bacteria in the human colon.
31896257	0	4	theme	Fermentation	58:69	arg1	Rate					71:74	the in Vitro Fecal Fermentation Rate	39:74	the in Vitro Fecal Fermentation Rate	39:74	Cell Wall Integrity of Pulse Modulates the in Vitro Fecal Fermentation Rate and Microbiota Composition.
31896257	3	5	theme	fermentation	619:630	arg1	rate					632:635	the in vitro fecal fermentation rate	600:635	the in vitro fecal fermentation rate of heat-treated pinto bean cells	600:668	In vitro human fecal fermentation performance and microbiota responses were tested, and we reported that the cell wall integrity controls the in vitro fecal fermentation rate of heat-treated pinto bean cells.
31896257	4	6	theme	bacterial	809:817	arg1	Roseburia					846:854	Roseburia	846:854	Roseburia	846:854	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	4	6	theme	bacterial	809:817	arg1	groups					819:824	certain beneficial bacterial groups	790:824	certain beneficial bacterial groups such as Blautia and Roseburia genera	790:861	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	4	6	theme	bacterial	809:817	arg1	Blautia					834:840	Blautia	834:840	Blautia	834:840	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	2	7	theme	intact	303:308	arg1	cells					320:324	intact cotyledon cells	303:324	intact cotyledon cells from pinto bean seeds	303:346	In the present study, we isolated intact cotyledon cells from pinto bean seeds as whole pulse food and RS 1 model and obtained a series of cell wall integrities through controlled enzymolysis.
31896257	3	8	dep	in	604:605	arg1	vitro					607:611	vitro	607:611	vitro	607:611	In vitro human fecal fermentation performance and microbiota responses were tested, and we reported that the cell wall integrity controls the in vitro fecal fermentation rate of heat-treated pinto bean cells.
31896257	0	9	theme	Microbiota	80:89	arg1	Composition					91:101	Microbiota Composition	80:101	Microbiota Composition	80:101	Cell Wall Integrity of Pulse Modulates the in Vitro Fecal Fermentation Rate and Microbiota Composition.
31896257	5	10	theme	intact	955:960	arg1	sample					967:972	the intact cell sample	951:972	the intact cell sample	951:972	However, the intact cell sample had a shape more similar to microbiota composition with the purified cell wall polysaccharides, rather than the damaged cells.
31896257	3	11	theme	fecal	477:481	arg1	performance					496:506	In vitro human fecal fermentation performance and microbiota responses	462:531	performance	496:506	In vitro human fecal fermentation performance and microbiota responses were tested, and we reported that the cell wall integrity controls the in vitro fecal fermentation rate of heat-treated pinto bean cells.
31896257	1	12	theme	specific	231:238	arg1	bacteria					240:247	specific bacteria	231:247	specific bacteria	231:247	The physical structure of type 1 resistant starch (RS 1) could influence the metabolite production and stimulate the growth of specific bacteria in the human colon.
31896257	3	13	theme	microbiota	512:521	arg1	responses					523:531	In vitro human fecal fermentation performance and microbiota responses	462:531	responses	523:531	In vitro human fecal fermentation performance and microbiota responses were tested, and we reported that the cell wall integrity controls the in vitro fecal fermentation rate of heat-treated pinto bean cells.
31896257	3	14	theme	cells	664:668	arg1	rate					632:635	the in vitro fecal fermentation rate	600:635	the in vitro fecal fermentation rate of heat-treated pinto bean cells	600:668	In vitro human fecal fermentation performance and microbiota responses were tested, and we reported that the cell wall integrity controls the in vitro fecal fermentation rate of heat-treated pinto bean cells.
31896257	3	15	theme	fermentation	483:494	arg1	performance					496:506	In vitro human fecal fermentation performance and microbiota responses	462:531	performance	496:506	In vitro human fecal fermentation performance and microbiota responses were tested, and we reported that the cell wall integrity controls the in vitro fecal fermentation rate of heat-treated pinto bean cells.
31896257	2	16	theme	cotyledon	310:318	arg1	cells					320:324	intact cotyledon cells	303:324	intact cotyledon cells from pinto bean seeds	303:346	In the present study, we isolated intact cotyledon cells from pinto bean seeds as whole pulse food and RS 1 model and obtained a series of cell wall integrities through controlled enzymolysis.
31896257	1	17	theme	bacteria	240:247	arg1	growth					221:226	the growth	217:226	the growth of specific bacteria in the human colon	217:266	The physical structure of type 1 resistant starch (RS 1) could influence the metabolite production and stimulate the growth of specific bacteria in the human colon.
31896257	5	18	theme	purified	1034:1041	arg1	polysaccharides					1053:1067	the purified cell wall polysaccharides	1030:1067	the purified cell wall polysaccharides	1030:1067	However, the intact cell sample had a shape more similar to microbiota composition with the purified cell wall polysaccharides, rather than the damaged cells.
31896257	4	19	theme	bean	719:722	arg1	fermentation					729:740	pinto bean cell fermentation	713:740	pinto bean cell fermentation	713:740	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	2	20	theme	controlled	438:447	arg1	enzymolysis					449:459	controlled enzymolysis	438:459	controlled enzymolysis	438:459	In the present study, we isolated intact cotyledon cells from pinto bean seeds as whole pulse food and RS 1 model and obtained a series of cell wall integrities through controlled enzymolysis.
31896257	3	21	theme	pinto	653:657	arg1	cells					664:668	heat-treated pinto bean cells	640:668	heat-treated pinto bean cells	640:668	In vitro human fecal fermentation performance and microbiota responses were tested, and we reported that the cell wall integrity controls the in vitro fecal fermentation rate of heat-treated pinto bean cells.
31896257	3	22	theme	human	471:475	arg1	performance					496:506	In vitro human fecal fermentation performance and microbiota responses	462:531	performance	496:506	In vitro human fecal fermentation performance and microbiota responses were tested, and we reported that the cell wall integrity controls the in vitro fecal fermentation rate of heat-treated pinto bean cells.
31896257	4	23	theme	damaged	913:919	arg1	integrity					931:939	damaged cell wall integrity	913:939	damaged cell wall integrity	913:939	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	0	24	theme	Wall	5:8	arg1	Integrity					10:18	Cell Wall Integrity	0:18	Cell Wall Integrity of Pulse	0:27	Cell Wall Integrity of Pulse Modulates the in Vitro Fecal Fermentation Rate and Microbiota Composition.
31896257	2	25	theme	cell	408:411	arg1	integrities					418:428	cell wall integrities	408:428	cell wall integrities	408:428	In the present study, we isolated intact cotyledon cells from pinto bean seeds as whole pulse food and RS 1 model and obtained a series of cell wall integrities through controlled enzymolysis.
31896257	4	26	theme	beneficial	798:807	arg1	Roseburia					846:854	Roseburia	846:854	Roseburia	846:854	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	4	26	theme	beneficial	798:807	arg1	groups					819:824	certain beneficial bacterial groups	790:824	certain beneficial bacterial groups such as Blautia and Roseburia genera	790:861	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	4	26	theme	beneficial	798:807	arg1	Blautia					834:840	Blautia	834:840	Blautia	834:840	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	4	27	theme	cell	921:924	arg1	integrity					931:939	damaged cell wall integrity	913:939	damaged cell wall integrity	913:939	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	2	28	theme	present	276:282	arg1	study					284:288	the present study	272:288	the present study	272:288	In the present study, we isolated intact cotyledon cells from pinto bean seeds as whole pulse food and RS 1 model and obtained a series of cell wall integrities through controlled enzymolysis.
31896257	2	29	theme	bean	337:340	arg1	seeds					342:346	pinto bean seeds	331:346	pinto bean seeds	331:346	In the present study, we isolated intact cotyledon cells from pinto bean seeds as whole pulse food and RS 1 model and obtained a series of cell wall integrities through controlled enzymolysis.
31896257	0	30	theme	Cell	0:3	arg1	Integrity					10:18	Cell Wall Integrity	0:18	Cell Wall Integrity of Pulse	0:27	Cell Wall Integrity of Pulse Modulates the in Vitro Fecal Fermentation Rate and Microbiota Composition.
31896257	3	31	theme	in	604:605	arg1	rate					632:635	the in vitro fecal fermentation rate	600:635	the in vitro fecal fermentation rate of heat-treated pinto bean cells	600:668	In vitro human fecal fermentation performance and microbiota responses were tested, and we reported that the cell wall integrity controls the in vitro fecal fermentation rate of heat-treated pinto bean cells.
31896257	5	32	with	composition	1013:1023	arg1	polysaccharides					1053:1067	the purified cell wall polysaccharides	1030:1067	the purified cell wall polysaccharides	1030:1067	However, the intact cell sample had a shape more similar to microbiota composition with the purified cell wall polysaccharides, rather than the damaged cells.
31896257	2	33	theme	pinto	331:335	arg1	seeds					342:346	pinto bean seeds	331:346	pinto bean seeds	331:346	In the present study, we isolated intact cotyledon cells from pinto bean seeds as whole pulse food and RS 1 model and obtained a series of cell wall integrities through controlled enzymolysis.
31896257	1	34	dep	type	130:133	arg1	RS					155:156	RS 1	155:158	RS 1	155:158	The physical structure of type 1 resistant starch (RS 1) could influence the metabolite production and stimulate the growth of specific bacteria in the human colon.
31896257	1	34	dep	type	130:133	arg1	starch					147:152	resistant starch	137:152	type 1 resistant starch (RS 1)	130:159	The physical structure of type 1 resistant starch (RS 1) could influence the metabolite production and stimulate the growth of specific bacteria in the human colon.
31896257	4	35	theme	cell	765:768	arg1	integrity					775:783	weakened cell wall integrity	756:783	weakened cell wall integrity	756:783	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	2	36	theme	RS	372:373	arg1	model					377:381	RS 1 model	372:381	RS 1 model	372:381	In the present study, we isolated intact cotyledon cells from pinto bean seeds as whole pulse food and RS 1 model and obtained a series of cell wall integrities through controlled enzymolysis.
31896257	1	37	theme	human	256:260	arg1	colon					262:266	the human colon	252:266	the human colon	252:266	The physical structure of type 1 resistant starch (RS 1) could influence the metabolite production and stimulate the growth of specific bacteria in the human colon.
31896257	3	38	dep	In	462:463	arg1	vitro					465:469	vitro	465:469	vitro	465:469	In vitro human fecal fermentation performance and microbiota responses were tested, and we reported that the cell wall integrity controls the in vitro fecal fermentation rate of heat-treated pinto bean cells.
31896257	1	39	from	growth	221:226	arg1	colon					262:266	the human colon	252:266	the human colon	252:266	The physical structure of type 1 resistant starch (RS 1) could influence the metabolite production and stimulate the growth of specific bacteria in the human colon.
31896257	5	40	theme	microbiota	1002:1011	arg1	composition					1013:1023	microbiota composition	1002:1023	microbiota composition with the purified cell wall polysaccharides	1002:1067	However, the intact cell sample had a shape more similar to microbiota composition with the purified cell wall polysaccharides, rather than the damaged cells.
31896257	0	41	dep	in	43:44	arg1	Vitro					46:50	Vitro	46:50	Vitro	46:50	Cell Wall Integrity of Pulse Modulates the in Vitro Fecal Fermentation Rate and Microbiota Composition.
31896257	2	42	theme	pulse	357:361	arg1	food					363:366	whole pulse food	351:366	whole pulse food	351:366	In the present study, we isolated intact cotyledon cells from pinto bean seeds as whole pulse food and RS 1 model and obtained a series of cell wall integrities through controlled enzymolysis.
31896257	0	43	theme	Pulse	23:27	arg1	Integrity					10:18	Cell Wall Integrity	0:18	Cell Wall Integrity of Pulse	0:27	Cell Wall Integrity of Pulse Modulates the in Vitro Fecal Fermentation Rate and Microbiota Composition.
31896257	4	44	theme	wall	926:929	arg1	integrity					931:939	damaged cell wall integrity	913:939	damaged cell wall integrity	913:939	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	3	45	theme	cell	571:574	arg1	integrity					581:589	the cell wall integrity	567:589	the cell wall integrity	567:589	In vitro human fecal fermentation performance and microbiota responses were tested, and we reported that the cell wall integrity controls the in vitro fecal fermentation rate of heat-treated pinto bean cells.
31896257	4	46	theme	weakened	756:763	arg1	integrity					775:783	weakened cell wall integrity	756:783	weakened cell wall integrity	756:783	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	1	47	theme	metabolite	181:190	arg1	production					192:201	the metabolite production	177:201	the metabolite production	177:201	The physical structure of type 1 resistant starch (RS 1) could influence the metabolite production and stimulate the growth of specific bacteria in the human colon.
31896257	0	48	theme	in	43:44	arg1	Rate					71:74	the in Vitro Fecal Fermentation Rate	39:74	the in Vitro Fecal Fermentation Rate	39:74	Cell Wall Integrity of Pulse Modulates the in Vitro Fecal Fermentation Rate and Microbiota Composition.
31896257	4	49	theme	bean	897:900	arg1	cells					902:906	pinto bean cells	891:906	pinto bean cells with damaged cell wall integrity	891:939	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	3	50	theme	wall	576:579	arg1	integrity					581:589	the cell wall integrity	567:589	the cell wall integrity	567:589	In vitro human fecal fermentation performance and microbiota responses were tested, and we reported that the cell wall integrity controls the in vitro fecal fermentation rate of heat-treated pinto bean cells.
31896257	4	51	theme	certain	790:796	arg1	Roseburia					846:854	Roseburia	846:854	Roseburia	846:854	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	4	51	theme	certain	790:796	arg1	groups					819:824	certain beneficial bacterial groups	790:824	certain beneficial bacterial groups such as Blautia and Roseburia genera	790:861	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	4	51	theme	certain	790:796	arg1	Blautia					834:840	Blautia	834:840	Blautia	834:840	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	4	52	dep	Blautia	834:840	arg1	genera					856:861	genera	856:861	genera	856:861	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	5	53	theme	wall	1048:1051	arg1	polysaccharides					1053:1067	the purified cell wall polysaccharides	1030:1067	the purified cell wall polysaccharides	1030:1067	However, the intact cell sample had a shape more similar to microbiota composition with the purified cell wall polysaccharides, rather than the damaged cells.
31896257	4	54	theme	wall	770:773	arg1	integrity					775:783	weakened cell wall integrity	756:783	weakened cell wall integrity	756:783	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	1	55	theme	physical	108:115	arg1	structure					117:125	The physical structure	104:125	The physical structure of type 1 resistant starch (RS 1)	104:159	The physical structure of type 1 resistant starch (RS 1) could influence the metabolite production and stimulate the growth of specific bacteria in the human colon.
31896257	5	56	contain	had	974:976	arg1	sample					967:972	the intact cell sample	951:972	the intact cell sample	951:972	However, the intact cell sample had a shape more similar to microbiota composition with the purified cell wall polysaccharides, rather than the damaged cells.
31896257	5	56	contain	had	974:976	arg2	shape					980:984	a shape	978:984	a shape more similar to microbiota composition with the purified cell wall polysaccharides	978:1067	However, the intact cell sample had a shape more similar to microbiota composition with the purified cell wall polysaccharides, rather than the damaged cells.
31896257	5	57	theme	similar	991:997	arg1	shape					980:984	a shape	978:984	a shape more similar to microbiota composition with the purified cell wall polysaccharides	978:1067	However, the intact cell sample had a shape more similar to microbiota composition with the purified cell wall polysaccharides, rather than the damaged cells.
31896257	5	58	theme	cell	962:965	arg1	sample					967:972	the intact cell sample	951:972	the intact cell sample	951:972	However, the intact cell sample had a shape more similar to microbiota composition with the purified cell wall polysaccharides, rather than the damaged cells.
31896257	3	59	theme	fecal	613:617	arg1	rate					632:635	the in vitro fecal fermentation rate	600:635	the in vitro fecal fermentation rate of heat-treated pinto bean cells	600:668	In vitro human fecal fermentation performance and microbiota responses were tested, and we reported that the cell wall integrity controls the in vitro fecal fermentation rate of heat-treated pinto bean cells.
31896257	2	60	from	seeds	342:346	arg1	cells					320:324	intact cotyledon cells	303:324	intact cotyledon cells from pinto bean seeds	303:346	In the present study, we isolated intact cotyledon cells from pinto bean seeds as whole pulse food and RS 1 model and obtained a series of cell wall integrities through controlled enzymolysis.
31896257	4	61	theme	butyrate	692:699	arg1	concentration					675:687	The concentration	671:687	The concentration of butyrate produced by pinto bean cell fermentation	671:740	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	4	62	theme	pinto	713:717	arg1	fermentation					729:740	pinto bean cell fermentation	713:740	pinto bean cell fermentation	713:740	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	4	63	theme	cell	724:727	arg1	fermentation					729:740	pinto bean cell fermentation	713:740	pinto bean cell fermentation	713:740	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	0	64	theme	Fecal	52:56	arg1	Rate					71:74	the in Vitro Fecal Fermentation Rate	39:74	the in Vitro Fecal Fermentation Rate	39:74	Cell Wall Integrity of Pulse Modulates the in Vitro Fecal Fermentation Rate and Microbiota Composition.
31896257	2	65	theme	whole	351:355	arg1	food					363:366	whole pulse food	351:366	whole pulse food	351:366	In the present study, we isolated intact cotyledon cells from pinto bean seeds as whole pulse food and RS 1 model and obtained a series of cell wall integrities through controlled enzymolysis.
31896257	4	66	with	cells	902:906	arg1	integrity					931:939	damaged cell wall integrity	913:939	damaged cell wall integrity	913:939	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
31896257	1	67	theme	type	130:133	arg1	structure					117:125	The physical structure	104:125	The physical structure of type 1 resistant starch (RS 1)	104:159	The physical structure of type 1 resistant starch (RS 1) could influence the metabolite production and stimulate the growth of specific bacteria in the human colon.
31896257	2	68	theme	integrities	418:428	arg1	series					398:403	a series	396:403	a series of cell wall integrities	396:428	In the present study, we isolated intact cotyledon cells from pinto bean seeds as whole pulse food and RS 1 model and obtained a series of cell wall integrities through controlled enzymolysis.
31896257	5	69	theme	damaged	1086:1092	arg1	cells					1094:1098	the damaged cells	1082:1098	the damaged cells	1082:1098	However, the intact cell sample had a shape more similar to microbiota composition with the purified cell wall polysaccharides, rather than the damaged cells.
31896257	5	70	theme	cell	1043:1046	arg1	polysaccharides					1053:1067	the purified cell wall polysaccharides	1030:1067	the purified cell wall polysaccharides	1030:1067	However, the intact cell sample had a shape more similar to microbiota composition with the purified cell wall polysaccharides, rather than the damaged cells.
31896257	3	71	theme	In	462:463	arg1	performance					496:506	In vitro human fecal fermentation performance and microbiota responses	462:531	performance	496:506	In vitro human fecal fermentation performance and microbiota responses were tested, and we reported that the cell wall integrity controls the in vitro fecal fermentation rate of heat-treated pinto bean cells.
31896257	4	72	theme	pinto	891:895	arg1	cells					902:906	pinto bean cells	891:906	pinto bean cells with damaged cell wall integrity	891:939	The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity.
34420728	7	0	theme	freeform	1140:1147	arg1	printing					1152:1159	freeform 3D printing	1140:1159	freeform 3D printing of diverse pseudoplastic biomaterial inks for biomedical applications	1140:1229	This method can facilitate freeform 3D printing of diverse pseudoplastic biomaterial inks for biomedical applications.
34420728	4	1	theme	CaCl2	630:634	arg1	cross-linking					652:664	CaCl2 diffusion-based cross-linking	630:664	CaCl2 diffusion-based cross-linking	630:664	The mixtures supported the shape of alginate and allowed CaCl2 diffusion-based cross-linking during 3D printing.
34420728	6	2	theme	alginate	1079:1086	arg1	concentration					1088:1100	alginate concentration	1079:1100	alginate concentration	1079:1100	BSA-loaded multi-layered spheres, freeform 3D-printed for oral protein drug delivery, protected BSA in the gastric environment and provided controlled and sustained release of BSA into the intestinal environment as layer width and alginate concentration increased.
34420728	6	3	theme	sustained	1003:1011	arg1	release					1013:1019	controlled and sustained release	988:1019	controlled and sustained release of BSA into the intestinal environment	988:1058	BSA-loaded multi-layered spheres, freeform 3D-printed for oral protein drug delivery, protected BSA in the gastric environment and provided controlled and sustained release of BSA into the intestinal environment as layer width and alginate concentration increased.
34420728	5	4	theme	physicochemical	723:737	arg1	properties					739:748	rheological and physicochemical properties	707:748	properties	739:748	The hydrogels showed rheological and physicochemical properties similar to those of pure alginate hydrogel, as CNCs were removed during post-printing processing.
34420728	1	5	from	fabrication	160:170	arg1	selection					97:105	The selection	93:105	The selection of sacrificial support materials	93:138	The selection of sacrificial support materials is important in the fabrication of complex freeform structures.
34420728	1	5	from	fabrication	160:170	arg1	important					143:151	important	143:151	important	143:151	The selection of sacrificial support materials is important in the fabrication of complex freeform structures.
34420728	3	6	theme	structural	551:560	arg1	stability					562:570	structural stability	551:570	structural stability	551:570	CNCs-CaCl2 mixture compositions and alginate concentrations were varied to enhance printability with rheological properties of shape fidelity and structural stability.
34420728	6	7	theme	oral	906:909	arg1	delivery					924:931	oral protein drug delivery	906:931	oral protein drug delivery	906:931	BSA-loaded multi-layered spheres, freeform 3D-printed for oral protein drug delivery, protected BSA in the gastric environment and provided controlled and sustained release of BSA into the intestinal environment as layer width and alginate concentration increased.
34420728	2	8	theme	biomaterial	296:306	arg1	inks					308:311	pseudoplastic alginate biomaterial inks	273:311	pseudoplastic alginate biomaterial inks	273:311	In this study, a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks was developed using Bingham plastic cellulose nanocrystals (CNCs) as support nanomaterials.
34420728	3	9	theme	mixture	416:422	arg1	compositions					424:435	CNCs-CaCl2 mixture compositions	405:435	CNCs-CaCl2 mixture compositions	405:435	CNCs-CaCl2 mixture compositions and alginate concentrations were varied to enhance printability with rheological properties of shape fidelity and structural stability.
34420728	6	10	theme	controlled	988:997	arg1	release					1013:1019	controlled and sustained release	988:1019	controlled and sustained release of BSA into the intestinal environment	988:1058	BSA-loaded multi-layered spheres, freeform 3D-printed for oral protein drug delivery, protected BSA in the gastric environment and provided controlled and sustained release of BSA into the intestinal environment as layer width and alginate concentration increased.
34420728	5	11	theme	similar	750:756	arg1	properties					739:748	rheological and physicochemical properties	707:748	properties	739:748	The hydrogels showed rheological and physicochemical properties similar to those of pure alginate hydrogel, as CNCs were removed during post-printing processing.
34420728	2	12	theme	alginate	287:294	arg1	inks					308:311	pseudoplastic alginate biomaterial inks	273:311	pseudoplastic alginate biomaterial inks	273:311	In this study, a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks was developed using Bingham plastic cellulose nanocrystals (CNCs) as support nanomaterials.
34420728	4	13	theme	diffusion-based	636:650	arg1	cross-linking					652:664	CaCl2 diffusion-based cross-linking	630:664	CaCl2 diffusion-based cross-linking	630:664	The mixtures supported the shape of alginate and allowed CaCl2 diffusion-based cross-linking during 3D printing.
34420728	3	14	theme	shape	532:536	arg1	fidelity					538:545	shape fidelity	532:545	shape fidelity	532:545	CNCs-CaCl2 mixture compositions and alginate concentrations were varied to enhance printability with rheological properties of shape fidelity and structural stability.
34420728	7	15	theme	biomedical	1207:1216	arg1	applications					1218:1229	biomedical applications	1207:1229	biomedical applications	1207:1229	This method can facilitate freeform 3D printing of diverse pseudoplastic biomaterial inks for biomedical applications.
34420728	6	16	theme	3D-printed	891:900	arg1	freeform					882:889	freeform	882:889	freeform 3D-printed for oral protein drug delivery	882:931	BSA-loaded multi-layered spheres, freeform 3D-printed for oral protein drug delivery, protected BSA in the gastric environment and provided controlled and sustained release of BSA into the intestinal environment as layer width and alginate concentration increased.
34420728	6	16	theme	3D-printed	891:900	arg1	spheres					873:879	BSA-loaded multi-layered spheres	848:879	BSA-loaded multi-layered spheres	848:879	BSA-loaded multi-layered spheres, freeform 3D-printed for oral protein drug delivery, protected BSA in the gastric environment and provided controlled and sustained release of BSA into the intestinal environment as layer width and alginate concentration increased.
34420728	2	17	theme	pseudoplastic	273:285	arg1	inks					308:311	pseudoplastic alginate biomaterial inks	273:311	pseudoplastic alginate biomaterial inks	273:311	In this study, a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks was developed using Bingham plastic cellulose nanocrystals (CNCs) as support nanomaterials.
34420728	1	18	theme	complex	175:181	arg1	structures					192:201	complex freeform structures	175:201	complex freeform structures	175:201	The selection of sacrificial support materials is important in the fabrication of complex freeform structures.
34420728	7	19	theme	biomaterial	1186:1196	arg1	inks					1198:1201	diverse pseudoplastic biomaterial inks	1164:1201	diverse pseudoplastic biomaterial inks	1164:1201	This method can facilitate freeform 3D printing of diverse pseudoplastic biomaterial inks for biomedical applications.
34420728	3	20	theme	CNCs-CaCl2	405:414	arg1	compositions					424:435	CNCs-CaCl2 mixture compositions	405:435	CNCs-CaCl2 mixture compositions	405:435	CNCs-CaCl2 mixture compositions and alginate concentrations were varied to enhance printability with rheological properties of shape fidelity and structural stability.
34420728	1	21	theme	freeform	183:190	arg1	structures					192:201	complex freeform structures	175:201	complex freeform structures	175:201	The selection of sacrificial support materials is important in the fabrication of complex freeform structures.
34420728	4	22	theme	alginate	609:616	arg1	shape					600:604	the shape	596:604	the shape of alginate	596:616	The mixtures supported the shape of alginate and allowed CaCl2 diffusion-based cross-linking during 3D printing.
34420728	7	23	theme	inks	1198:1201	arg1	printing					1152:1159	freeform 3D printing	1140:1159	freeform 3D printing of diverse pseudoplastic biomaterial inks for biomedical applications	1140:1229	This method can facilitate freeform 3D printing of diverse pseudoplastic biomaterial inks for biomedical applications.
34420728	1	24	from	important	143:151	arg1	fabrication					160:170	the fabrication	156:170	the fabrication of complex freeform structures	156:201	The selection of sacrificial support materials is important in the fabrication of complex freeform structures.
34420728	1	25	theme	structures	192:201	arg1	fabrication					160:170	the fabrication	156:170	the fabrication of complex freeform structures	156:201	The selection of sacrificial support materials is important in the fabrication of complex freeform structures.
34420728	0	26	theme	Cellulose	0:8	arg1	nanocrystals					10:21	Cellulose nanocrystals	0:21	Cellulose nanocrystals as	0:24	Cellulose nanocrystals as support nanomaterials for dual droplet-based freeform 3D printing.
34420728	5	27	theme	rheological	707:717	arg1	properties					739:748	rheological and physicochemical properties	707:748	properties	739:748	The hydrogels showed rheological and physicochemical properties similar to those of pure alginate hydrogel, as CNCs were removed during post-printing processing.
34420728	2	28	theme	support	382:388	arg1	nanocrystals					359:370	Bingham plastic cellulose nanocrystals	333:370	Bingham plastic cellulose nanocrystals (CNCs)	333:377	In this study, a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks was developed using Bingham plastic cellulose nanocrystals (CNCs) as support nanomaterials.
34420728	2	28	theme	support	382:388	arg1	nanomaterials					390:402	support nanomaterials	382:402	support nanomaterials	382:402	In this study, a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks was developed using Bingham plastic cellulose nanocrystals (CNCs) as support nanomaterials.
34420728	6	29	theme	layer	1063:1067	arg1	width					1069:1073	layer width	1063:1073	layer width	1063:1073	BSA-loaded multi-layered spheres, freeform 3D-printed for oral protein drug delivery, protected BSA in the gastric environment and provided controlled and sustained release of BSA into the intestinal environment as layer width and alginate concentration increased.
34420728	5	30	theme	alginate	775:782	arg1	hydrogel					784:791	pure alginate hydrogel	770:791	pure alginate hydrogel	770:791	The hydrogels showed rheological and physicochemical properties similar to those of pure alginate hydrogel, as CNCs were removed during post-printing processing.
34420728	6	31	theme	multi-layered	859:871	arg1	freeform					882:889	freeform	882:889	freeform 3D-printed for oral protein drug delivery	882:931	BSA-loaded multi-layered spheres, freeform 3D-printed for oral protein drug delivery, protected BSA in the gastric environment and provided controlled and sustained release of BSA into the intestinal environment as layer width and alginate concentration increased.
34420728	6	31	theme	multi-layered	859:871	arg1	spheres					873:879	BSA-loaded multi-layered spheres	848:879	BSA-loaded multi-layered spheres	848:879	BSA-loaded multi-layered spheres, freeform 3D-printed for oral protein drug delivery, protected BSA in the gastric environment and provided controlled and sustained release of BSA into the intestinal environment as layer width and alginate concentration increased.
34420728	6	32	theme	protein	911:917	arg1	delivery					924:931	oral protein drug delivery	906:931	oral protein drug delivery	906:931	BSA-loaded multi-layered spheres, freeform 3D-printed for oral protein drug delivery, protected BSA in the gastric environment and provided controlled and sustained release of BSA into the intestinal environment as layer width and alginate concentration increased.
34420728	3	33	with	printability	488:499	arg1	properties					518:527	rheological properties	506:527	rheological properties of shape fidelity and structural stability	506:570	CNCs-CaCl2 mixture compositions and alginate concentrations were varied to enhance printability with rheological properties of shape fidelity and structural stability.
34420728	6	34	theme	gastric	955:961	arg1	environment					963:973	the gastric environment	951:973	the gastric environment	951:973	BSA-loaded multi-layered spheres, freeform 3D-printed for oral protein drug delivery, protected BSA in the gastric environment and provided controlled and sustained release of BSA into the intestinal environment as layer width and alginate concentration increased.
34420728	1	35	theme	sacrificial	110:120	arg1	materials					130:138	sacrificial support materials	110:138	sacrificial support materials	110:138	The selection of sacrificial support materials is important in the fabrication of complex freeform structures.
34420728	3	36	theme	rheological	506:516	arg1	properties					518:527	rheological properties	506:527	rheological properties of shape fidelity and structural stability	506:570	CNCs-CaCl2 mixture compositions and alginate concentrations were varied to enhance printability with rheological properties of shape fidelity and structural stability.
34420728	2	37	theme	cellulose	349:357	arg1	CNCs					373:376	CNCs	373:376	CNCs	373:376	In this study, a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks was developed using Bingham plastic cellulose nanocrystals (CNCs) as support nanomaterials.
34420728	2	37	theme	cellulose	349:357	arg1	nanocrystals					359:370	Bingham plastic cellulose nanocrystals	333:370	Bingham plastic cellulose nanocrystals (CNCs)	333:377	In this study, a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks was developed using Bingham plastic cellulose nanocrystals (CNCs) as support nanomaterials.
34420728	2	37	theme	cellulose	349:357	arg1	nanomaterials					390:402	support nanomaterials	382:402	support nanomaterials	382:402	In this study, a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks was developed using Bingham plastic cellulose nanocrystals (CNCs) as support nanomaterials.
34420728	5	38	theme	post-printing	822:834	arg1	processing					836:845	post-printing processing	822:845	post-printing processing	822:845	The hydrogels showed rheological and physicochemical properties similar to those of pure alginate hydrogel, as CNCs were removed during post-printing processing.
34420728	3	39	theme	alginate	441:448	arg1	concentrations					450:463	alginate concentrations	441:463	alginate concentrations	441:463	CNCs-CaCl2 mixture compositions and alginate concentrations were varied to enhance printability with rheological properties of shape fidelity and structural stability.
34420728	6	40	theme	intestinal	1037:1046	arg1	environment					1048:1058	the intestinal environment	1033:1058	the intestinal environment	1033:1058	BSA-loaded multi-layered spheres, freeform 3D-printed for oral protein drug delivery, protected BSA in the gastric environment and provided controlled and sustained release of BSA into the intestinal environment as layer width and alginate concentration increased.
34420728	7	41	theme	diverse	1164:1170	arg1	inks					1198:1201	diverse pseudoplastic biomaterial inks	1164:1201	diverse pseudoplastic biomaterial inks	1164:1201	This method can facilitate freeform 3D printing of diverse pseudoplastic biomaterial inks for biomedical applications.
34420728	2	42	theme	droplet-based	226:238	arg1	method					262:267	a dual droplet-based, freeform 3D printing method	219:267	a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks	219:311	In this study, a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks was developed using Bingham plastic cellulose nanocrystals (CNCs) as support nanomaterials.
34420728	6	43	theme	BSA-loaded	848:857	arg1	freeform					882:889	freeform	882:889	freeform 3D-printed for oral protein drug delivery	882:931	BSA-loaded multi-layered spheres, freeform 3D-printed for oral protein drug delivery, protected BSA in the gastric environment and provided controlled and sustained release of BSA into the intestinal environment as layer width and alginate concentration increased.
34420728	6	43	theme	BSA-loaded	848:857	arg1	spheres					873:879	BSA-loaded multi-layered spheres	848:879	BSA-loaded multi-layered spheres	848:879	BSA-loaded multi-layered spheres, freeform 3D-printed for oral protein drug delivery, protected BSA in the gastric environment and provided controlled and sustained release of BSA into the intestinal environment as layer width and alginate concentration increased.
34420728	2	44	dep	droplet-based	226:238	arg1	freeform					241:248	freeform	241:248	freeform	241:248	In this study, a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks was developed using Bingham plastic cellulose nanocrystals (CNCs) as support nanomaterials.
34420728	0	45	theme	droplet-based	57:69	arg1	printing					83:90	dual droplet-based freeform 3D printing	52:90	dual droplet-based freeform 3D printing	52:90	Cellulose nanocrystals as support nanomaterials for dual droplet-based freeform 3D printing.
34420728	7	46	theme	3D	1149:1150	arg1	printing					1152:1159	freeform 3D printing	1140:1159	freeform 3D printing of diverse pseudoplastic biomaterial inks for biomedical applications	1140:1229	This method can facilitate freeform 3D printing of diverse pseudoplastic biomaterial inks for biomedical applications.
34420728	2	47	theme	dual	221:224	arg1	method					262:267	a dual droplet-based, freeform 3D printing method	219:267	a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks	219:311	In this study, a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks was developed using Bingham plastic cellulose nanocrystals (CNCs) as support nanomaterials.
34420728	1	48	theme	support	122:128	arg1	materials					130:138	sacrificial support materials	110:138	sacrificial support materials	110:138	The selection of sacrificial support materials is important in the fabrication of complex freeform structures.
34420728	0	49	theme	dual	52:55	arg1	printing					83:90	dual droplet-based freeform 3D printing	52:90	dual droplet-based freeform 3D printing	52:90	Cellulose nanocrystals as support nanomaterials for dual droplet-based freeform 3D printing.
34420728	7	50	theme	pseudoplastic	1172:1184	arg1	inks					1198:1201	diverse pseudoplastic biomaterial inks	1164:1201	diverse pseudoplastic biomaterial inks	1164:1201	This method can facilitate freeform 3D printing of diverse pseudoplastic biomaterial inks for biomedical applications.
34420728	1	51	theme	materials	130:138	arg1	selection					97:105	The selection	93:105	The selection of sacrificial support materials	93:138	The selection of sacrificial support materials is important in the fabrication of complex freeform structures.
34420728	1	51	theme	materials	130:138	arg1	important					143:151	important	143:151	important	143:151	The selection of sacrificial support materials is important in the fabrication of complex freeform structures.
34420728	6	52	theme	BSA	1024:1026	arg1	release					1013:1019	controlled and sustained release	988:1019	controlled and sustained release of BSA into the intestinal environment	988:1058	BSA-loaded multi-layered spheres, freeform 3D-printed for oral protein drug delivery, protected BSA in the gastric environment and provided controlled and sustained release of BSA into the intestinal environment as layer width and alginate concentration increased.
34420728	2	53	theme	plastic	341:347	arg1	CNCs					373:376	CNCs	373:376	CNCs	373:376	In this study, a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks was developed using Bingham plastic cellulose nanocrystals (CNCs) as support nanomaterials.
34420728	2	53	theme	plastic	341:347	arg1	nanocrystals					359:370	Bingham plastic cellulose nanocrystals	333:370	Bingham plastic cellulose nanocrystals (CNCs)	333:377	In this study, a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks was developed using Bingham plastic cellulose nanocrystals (CNCs) as support nanomaterials.
34420728	2	53	theme	plastic	341:347	arg1	nanomaterials					390:402	support nanomaterials	382:402	support nanomaterials	382:402	In this study, a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks was developed using Bingham plastic cellulose nanocrystals (CNCs) as support nanomaterials.
34420728	4	54	theme	3D	673:674	arg1	printing					676:683	3D printing	673:683	3D printing	673:683	The mixtures supported the shape of alginate and allowed CaCl2 diffusion-based cross-linking during 3D printing.
34420728	5	55	theme	pure	770:773	arg1	hydrogel					784:791	pure alginate hydrogel	770:791	pure alginate hydrogel	770:791	The hydrogels showed rheological and physicochemical properties similar to those of pure alginate hydrogel, as CNCs were removed during post-printing processing.
34420728	2	56	theme	printing	253:260	arg1	method					262:267	a dual droplet-based, freeform 3D printing method	219:267	a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks	219:311	In this study, a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks was developed using Bingham plastic cellulose nanocrystals (CNCs) as support nanomaterials.
34420728	2	57	theme	Bingham	333:339	arg1	CNCs					373:376	CNCs	373:376	CNCs	373:376	In this study, a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks was developed using Bingham plastic cellulose nanocrystals (CNCs) as support nanomaterials.
34420728	2	57	theme	Bingham	333:339	arg1	nanocrystals					359:370	Bingham plastic cellulose nanocrystals	333:370	Bingham plastic cellulose nanocrystals (CNCs)	333:377	In this study, a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks was developed using Bingham plastic cellulose nanocrystals (CNCs) as support nanomaterials.
34420728	2	57	theme	Bingham	333:339	arg1	nanomaterials					390:402	support nanomaterials	382:402	support nanomaterials	382:402	In this study, a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks was developed using Bingham plastic cellulose nanocrystals (CNCs) as support nanomaterials.
34420728	0	58	theme	3D	80:81	arg1	printing					83:90	dual droplet-based freeform 3D printing	52:90	dual droplet-based freeform 3D printing	52:90	Cellulose nanocrystals as support nanomaterials for dual droplet-based freeform 3D printing.
34420728	6	59	theme	drug	919:922	arg1	delivery					924:931	oral protein drug delivery	906:931	oral protein drug delivery	906:931	BSA-loaded multi-layered spheres, freeform 3D-printed for oral protein drug delivery, protected BSA in the gastric environment and provided controlled and sustained release of BSA into the intestinal environment as layer width and alginate concentration increased.
34420728	2	60	theme	3D	250:251	arg1	method					262:267	a dual droplet-based, freeform 3D printing method	219:267	a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks	219:311	In this study, a dual droplet-based, freeform 3D printing method for pseudoplastic alginate biomaterial inks was developed using Bingham plastic cellulose nanocrystals (CNCs) as support nanomaterials.
34420728	3	61	theme	stability	562:570	arg1	properties					518:527	rheological properties	506:527	rheological properties of shape fidelity and structural stability	506:570	CNCs-CaCl2 mixture compositions and alginate concentrations were varied to enhance printability with rheological properties of shape fidelity and structural stability.
34420728	0	62	theme	freeform	71:78	arg1	printing					83:90	dual droplet-based freeform 3D printing	52:90	dual droplet-based freeform 3D printing	52:90	Cellulose nanocrystals as support nanomaterials for dual droplet-based freeform 3D printing.
34420728	3	63	theme	fidelity	538:545	arg1	properties					518:527	rheological properties	506:527	rheological properties of shape fidelity and structural stability	506:570	CNCs-CaCl2 mixture compositions and alginate concentrations were varied to enhance printability with rheological properties of shape fidelity and structural stability.
31925335	0	0	theme	electron	89:96	arg1	tomography					98:107	freeze-etching electron tomography	74:107	freeze-etching electron tomography	74:107	Nanoarchitecture and dynamics of the mouse enteric glycocalyx examined by freeze-etching electron tomography and intravital microscopy.
31925335	6	1	with	network	890:896	arg1	mesh					911:914	a 30 nm mesh	903:914	a 30 nm mesh	903:914	Micrometer-long mucin filaments emerge from microvillar-tips and, through zigzagged lateral interactions form a three-dimensional columnar network with a 30 nm mesh.
31925335	5	2	theme	electron	730:737	arg1	tomography					739:748	freeze-etching and electron tomography	711:748	tomography	739:748	Here, we characterize the nanoarchitecture of the murine enteric glycocalyx using freeze-etching and electron tomography.
31925335	6	3	theme	zigzagged	825:833	arg1	interactions					843:854	zigzagged lateral interactions	825:854	zigzagged lateral interactions	825:854	Micrometer-long mucin filaments emerge from microvillar-tips and, through zigzagged lateral interactions form a three-dimensional columnar network with a 30 nm mesh.
31925335	1	4	theme	hydrated	163:170	arg1	glycocalyx					140:149	The glycocalyx	136:149	The glycocalyx	136:149	The glycocalyx is a highly hydrated, glycoprotein-rich coat shrouding many eukaryotic and prokaryotic cells.
31925335	1	4	theme	hydrated	163:170	arg1	coat					191:194	a highly hydrated, glycoprotein-rich coat	154:194	a highly hydrated, glycoprotein-rich coat shrouding many eukaryotic and prokaryotic cells	154:242	The glycocalyx is a highly hydrated, glycoprotein-rich coat shrouding many eukaryotic and prokaryotic cells.
31925335	0	5	theme	freeze-etching	74:87	arg1	tomography					98:107	freeze-etching electron tomography	74:107	freeze-etching electron tomography	74:107	Nanoarchitecture and dynamics of the mouse enteric glycocalyx examined by freeze-etching electron tomography and intravital microscopy.
31925335	8	6	dep	glycocalyx	1058:1067	arg1	deformability					1069:1081	deformability	1069:1081	deformability	1069:1081	Finally, we assess glycocalyx deformability and porosity using intravital microscopy.
31925335	8	6	dep	glycocalyx	1058:1067	arg1	glycocalyx					1058:1067	glycocalyx deformability and porosity	1058:1094	glycocalyx deformability and porosity	1058:1094	Finally, we assess glycocalyx deformability and porosity using intravital microscopy.
31925335	8	6	dep	glycocalyx	1058:1067	arg1	porosity					1087:1094	porosity	1087:1094	porosity	1087:1094	Finally, we assess glycocalyx deformability and porosity using intravital microscopy.
31925335	8	7	theme	intravital	1102:1111	arg1	microscopy					1113:1122	intravital microscopy	1102:1122	intravital microscopy	1102:1122	Finally, we assess glycocalyx deformability and porosity using intravital microscopy.
31925335	2	8	theme	primary	344:350	arg1	interface					365:373	the primary host-microbe interface	340:373	the primary host-microbe interface	340:373	The intestinal epithelial glycocalyx, comprising glycosylated transmembrane mucins, is part of the primary host-microbe interface and is essential for nutrient absorption.
31925335	5	9	theme	murine	679:684	arg1	glycocalyx					694:703	the murine enteric glycocalyx	675:703	the murine enteric glycocalyx	675:703	Here, we characterize the nanoarchitecture of the murine enteric glycocalyx using freeze-etching and electron tomography.
31925335	5	10	theme	glycocalyx	694:703	arg1	nanoarchitecture					655:670	the nanoarchitecture	651:670	the nanoarchitecture of the murine enteric glycocalyx	651:703	Here, we characterize the nanoarchitecture of the murine enteric glycocalyx using freeze-etching and electron tomography.
31925335	5	11	theme	enteric	686:692	arg1	glycocalyx					694:703	the murine enteric glycocalyx	675:703	the murine enteric glycocalyx	675:703	Here, we characterize the nanoarchitecture of the murine enteric glycocalyx using freeze-etching and electron tomography.
31925335	0	12	theme	intravital	113:122	arg1	microscopy					124:133	intravital microscopy	113:133	intravital microscopy	113:133	Nanoarchitecture and dynamics of the mouse enteric glycocalyx examined by freeze-etching electron tomography and intravital microscopy.
31925335	2	13	theme	transmembrane	307:319	arg1	mucins					321:326	glycosylated transmembrane mucins	294:326	glycosylated transmembrane mucins	294:326	The intestinal epithelial glycocalyx, comprising glycosylated transmembrane mucins, is part of the primary host-microbe interface and is essential for nutrient absorption.
31925335	5	14	theme	freeze-etching	711:724	arg1	tomography					739:748	freeze-etching and electron tomography	711:748	tomography	739:748	Here, we characterize the nanoarchitecture of the murine enteric glycocalyx using freeze-etching and electron tomography.
31925335	6	15	theme	30 nm	905:909	arg1	mesh					911:914	a 30 nm mesh	903:914	a 30 nm mesh	903:914	Micrometer-long mucin filaments emerge from microvillar-tips and, through zigzagged lateral interactions form a three-dimensional columnar network with a 30 nm mesh.
31925335	1	16	dep	hydrated	163:170	arg1	glycoprotein-rich					173:189	glycoprotein-rich	173:189	glycoprotein-rich	173:189	The glycocalyx is a highly hydrated, glycoprotein-rich coat shrouding many eukaryotic and prokaryotic cells.
31925335	3	17	theme	gastrointestinal	464:479	arg1	diseases					481:488	numerous gastrointestinal diseases	455:488	numerous gastrointestinal diseases	455:488	Its disruption has been implicated in numerous gastrointestinal diseases.
31925335	2	18	gly	glycosylated	294:305	arg1	mucins					321:326	glycosylated transmembrane mucins	294:326	glycosylated transmembrane mucins	294:326	The intestinal epithelial glycocalyx, comprising glycosylated transmembrane mucins, is part of the primary host-microbe interface and is essential for nutrient absorption.
31925335	7	19	theme	~30 nm	968:973	arg1	structures					957:966	globular structures	948:966	globular structures ~30 nm apart that are liquid-crystalline packed within a single plane	948:1036	Filament-termini converge into globular structures ~30 nm apart that are liquid-crystalline packed within a single plane.
31925335	1	20	theme	many	206:209	arg1	cells					238:242	many eukaryotic and prokaryotic cells	206:242	many eukaryotic and prokaryotic cells	206:242	The glycocalyx is a highly hydrated, glycoprotein-rich coat shrouding many eukaryotic and prokaryotic cells.
31925335	4	21	from	challenges	503:512	arg1	visualizing					532:542	visualizing	532:542	visualizing	532:542	Yet, due to challenges in preserving and visualizing its native organization, glycocalyx structure-function relationships remain unclear.
31925335	4	21	from	challenges	503:512	arg1	preserving					517:526	preserving	517:526	preserving	517:526	Yet, due to challenges in preserving and visualizing its native organization, glycocalyx structure-function relationships remain unclear.
31925335	1	22	theme	eukaryotic	211:220	arg1	cells					238:242	many eukaryotic and prokaryotic cells	206:242	many eukaryotic and prokaryotic cells	206:242	The glycocalyx is a highly hydrated, glycoprotein-rich coat shrouding many eukaryotic and prokaryotic cells.
31925335	4	23	dep	preserving	517:526	arg1	organization					555:566	its native organization	544:566	its native organization	544:566	Yet, due to challenges in preserving and visualizing its native organization, glycocalyx structure-function relationships remain unclear.
31925335	9	24	theme	deformable	1270:1279	arg1	glycocalyx					1242:1251	the glycocalyx	1238:1251	the glycocalyx	1238:1251	We argue that the columnar network architecture and the liquid-crystalline packing of the filament termini allow the glycocalyx to function as a deformable size-exclusion filter of luminal contents.
31925335	9	24	theme	deformable	1270:1279	arg1	filter					1296:1301	a deformable size-exclusion filter	1268:1301	a deformable size-exclusion filter of luminal contents	1268:1321	We argue that the columnar network architecture and the liquid-crystalline packing of the filament termini allow the glycocalyx to function as a deformable size-exclusion filter of luminal contents.
31925335	7	25	theme	globular	948:955	arg1	structures					957:966	globular structures	948:966	globular structures ~30 nm apart that are liquid-crystalline packed within a single plane	948:1036	Filament-termini converge into globular structures ~30 nm apart that are liquid-crystalline packed within a single plane.
31925335	0	26	theme	mouse	37:41	arg1	glycocalyx					51:60	the mouse enteric glycocalyx	33:60	the mouse enteric glycocalyx	33:60	Nanoarchitecture and dynamics of the mouse enteric glycocalyx examined by freeze-etching electron tomography and intravital microscopy.
31925335	9	27	theme	filament	1215:1222	arg1	termini					1224:1230	the filament termini	1211:1230	the filament termini	1211:1230	We argue that the columnar network architecture and the liquid-crystalline packing of the filament termini allow the glycocalyx to function as a deformable size-exclusion filter of luminal contents.
31925335	2	28	theme	epithelial	260:269	arg1	essential					382:390	essential	382:390	essential	382:390	The intestinal epithelial glycocalyx, comprising glycosylated transmembrane mucins, is part of the primary host-microbe interface and is essential for nutrient absorption.
31925335	2	28	theme	epithelial	260:269	arg1	glycocalyx					271:280	The intestinal epithelial glycocalyx	245:280	The intestinal epithelial glycocalyx	245:280	The intestinal epithelial glycocalyx, comprising glycosylated transmembrane mucins, is part of the primary host-microbe interface and is essential for nutrient absorption.
31925335	2	28	theme	epithelial	260:269	arg1	part					332:335	part	332:335	part of the primary host-microbe interface	332:373	The intestinal epithelial glycocalyx, comprising glycosylated transmembrane mucins, is part of the primary host-microbe interface and is essential for nutrient absorption.
31925335	6	29	theme	mucin	767:771	arg1	filaments					773:781	Micrometer-long mucin filaments	751:781	Micrometer-long mucin filaments	751:781	Micrometer-long mucin filaments emerge from microvillar-tips and, through zigzagged lateral interactions form a three-dimensional columnar network with a 30 nm mesh.
31925335	1	30	theme	prokaryotic	226:236	arg1	cells					238:242	many eukaryotic and prokaryotic cells	206:242	many eukaryotic and prokaryotic cells	206:242	The glycocalyx is a highly hydrated, glycoprotein-rich coat shrouding many eukaryotic and prokaryotic cells.
31925335	3	31	theme	numerous	455:462	arg1	diseases					481:488	numerous gastrointestinal diseases	455:488	numerous gastrointestinal diseases	455:488	Its disruption has been implicated in numerous gastrointestinal diseases.
31925335	4	32	theme	structure-function	580:597	arg1	relationships					599:611	glycocalyx structure-function relationships	569:611	glycocalyx structure-function relationships	569:611	Yet, due to challenges in preserving and visualizing its native organization, glycocalyx structure-function relationships remain unclear.
31925335	9	33	theme	columnar	1143:1150	arg1	architecture					1160:1171	the columnar network architecture	1139:1171	the columnar network architecture	1139:1171	We argue that the columnar network architecture and the liquid-crystalline packing of the filament termini allow the glycocalyx to function as a deformable size-exclusion filter of luminal contents.
31925335	2	34	theme	intestinal	249:258	arg1	essential					382:390	essential	382:390	essential	382:390	The intestinal epithelial glycocalyx, comprising glycosylated transmembrane mucins, is part of the primary host-microbe interface and is essential for nutrient absorption.
31925335	2	34	theme	intestinal	249:258	arg1	glycocalyx					271:280	The intestinal epithelial glycocalyx	245:280	The intestinal epithelial glycocalyx	245:280	The intestinal epithelial glycocalyx, comprising glycosylated transmembrane mucins, is part of the primary host-microbe interface and is essential for nutrient absorption.
31925335	2	34	theme	intestinal	249:258	arg1	part					332:335	part	332:335	part of the primary host-microbe interface	332:373	The intestinal epithelial glycocalyx, comprising glycosylated transmembrane mucins, is part of the primary host-microbe interface and is essential for nutrient absorption.
31925335	9	35	theme	size-exclusion	1281:1294	arg1	glycocalyx					1242:1251	the glycocalyx	1238:1251	the glycocalyx	1238:1251	We argue that the columnar network architecture and the liquid-crystalline packing of the filament termini allow the glycocalyx to function as a deformable size-exclusion filter of luminal contents.
31925335	9	35	theme	size-exclusion	1281:1294	arg1	filter					1296:1301	a deformable size-exclusion filter	1268:1301	a deformable size-exclusion filter of luminal contents	1268:1321	We argue that the columnar network architecture and the liquid-crystalline packing of the filament termini allow the glycocalyx to function as a deformable size-exclusion filter of luminal contents.
31925335	0	36	theme	glycocalyx	51:60	arg1	dynamics					21:28	dynamics	21:28	dynamics	21:28	Nanoarchitecture and dynamics of the mouse enteric glycocalyx examined by freeze-etching electron tomography and intravital microscopy.
31925335	0	36	theme	glycocalyx	51:60	arg1	Nanoarchitecture					0:15	Nanoarchitecture	0:15	Nanoarchitecture	0:15	Nanoarchitecture and dynamics of the mouse enteric glycocalyx examined by freeze-etching electron tomography and intravital microscopy.
31925335	6	37	theme	columnar	881:888	arg1	network					890:896	a three-dimensional columnar network	861:896	a three-dimensional columnar network with a 30 nm mesh	861:914	Micrometer-long mucin filaments emerge from microvillar-tips and, through zigzagged lateral interactions form a three-dimensional columnar network with a 30 nm mesh.
31925335	0	38	theme	enteric	43:49	arg1	glycocalyx					51:60	the mouse enteric glycocalyx	33:60	the mouse enteric glycocalyx	33:60	Nanoarchitecture and dynamics of the mouse enteric glycocalyx examined by freeze-etching electron tomography and intravital microscopy.
31925335	6	39	theme	three-dimensional	863:879	arg1	network					890:896	a three-dimensional columnar network	861:896	a three-dimensional columnar network with a 30 nm mesh	861:914	Micrometer-long mucin filaments emerge from microvillar-tips and, through zigzagged lateral interactions form a three-dimensional columnar network with a 30 nm mesh.
31925335	6	40	theme	Micrometer-long	751:765	arg1	filaments					773:781	Micrometer-long mucin filaments	751:781	Micrometer-long mucin filaments	751:781	Micrometer-long mucin filaments emerge from microvillar-tips and, through zigzagged lateral interactions form a three-dimensional columnar network with a 30 nm mesh.
31925335	2	41	theme	glycosylated	294:305	arg1	mucins					321:326	glycosylated transmembrane mucins	294:326	glycosylated transmembrane mucins	294:326	The intestinal epithelial glycocalyx, comprising glycosylated transmembrane mucins, is part of the primary host-microbe interface and is essential for nutrient absorption.
31925335	7	42	dep	packed	1009:1014	arg1	liquid-crystalline					990:1007	liquid-crystalline	990:1007	liquid-crystalline	990:1007	Filament-termini converge into globular structures ~30 nm apart that are liquid-crystalline packed within a single plane.
31925335	2	43	theme	interface	365:373	arg1	essential					382:390	essential	382:390	essential	382:390	The intestinal epithelial glycocalyx, comprising glycosylated transmembrane mucins, is part of the primary host-microbe interface and is essential for nutrient absorption.
31925335	2	43	theme	interface	365:373	arg1	glycocalyx					271:280	The intestinal epithelial glycocalyx	245:280	The intestinal epithelial glycocalyx	245:280	The intestinal epithelial glycocalyx, comprising glycosylated transmembrane mucins, is part of the primary host-microbe interface and is essential for nutrient absorption.
31925335	2	43	theme	interface	365:373	arg1	part					332:335	part	332:335	part of the primary host-microbe interface	332:373	The intestinal epithelial glycocalyx, comprising glycosylated transmembrane mucins, is part of the primary host-microbe interface and is essential for nutrient absorption.
31925335	9	44	theme	luminal	1306:1312	arg1	contents					1314:1321	luminal contents	1306:1321	luminal contents	1306:1321	We argue that the columnar network architecture and the liquid-crystalline packing of the filament termini allow the glycocalyx to function as a deformable size-exclusion filter of luminal contents.
31925335	9	45	theme	contents	1314:1321	arg1	glycocalyx					1242:1251	the glycocalyx	1238:1251	the glycocalyx	1238:1251	We argue that the columnar network architecture and the liquid-crystalline packing of the filament termini allow the glycocalyx to function as a deformable size-exclusion filter of luminal contents.
31925335	9	45	theme	contents	1314:1321	arg1	filter					1296:1301	a deformable size-exclusion filter	1268:1301	a deformable size-exclusion filter of luminal contents	1268:1321	We argue that the columnar network architecture and the liquid-crystalline packing of the filament termini allow the glycocalyx to function as a deformable size-exclusion filter of luminal contents.
31925335	2	46	theme	host-microbe	352:363	arg1	interface					365:373	the primary host-microbe interface	340:373	the primary host-microbe interface	340:373	The intestinal epithelial glycocalyx, comprising glycosylated transmembrane mucins, is part of the primary host-microbe interface and is essential for nutrient absorption.
31925335	9	47	theme	liquid-crystalline	1181:1198	arg1	packing					1200:1206	the liquid-crystalline packing	1177:1206	the liquid-crystalline packing of the filament termini	1177:1230	We argue that the columnar network architecture and the liquid-crystalline packing of the filament termini allow the glycocalyx to function as a deformable size-exclusion filter of luminal contents.
31925335	9	48	theme	termini	1224:1230	arg1	packing					1200:1206	the liquid-crystalline packing	1177:1206	the liquid-crystalline packing of the filament termini	1177:1230	We argue that the columnar network architecture and the liquid-crystalline packing of the filament termini allow the glycocalyx to function as a deformable size-exclusion filter of luminal contents.
31925335	9	48	theme	termini	1224:1230	arg1	architecture					1160:1171	the columnar network architecture	1139:1171	the columnar network architecture	1139:1171	We argue that the columnar network architecture and the liquid-crystalline packing of the filament termini allow the glycocalyx to function as a deformable size-exclusion filter of luminal contents.
31925335	9	49	theme	network	1152:1158	arg1	architecture					1160:1171	the columnar network architecture	1139:1171	the columnar network architecture	1139:1171	We argue that the columnar network architecture and the liquid-crystalline packing of the filament termini allow the glycocalyx to function as a deformable size-exclusion filter of luminal contents.
31925335	4	50	theme	native	548:553	arg1	organization					555:566	its native organization	544:566	its native organization	544:566	Yet, due to challenges in preserving and visualizing its native organization, glycocalyx structure-function relationships remain unclear.
31925335	6	51	theme	lateral	835:841	arg1	interactions					843:854	zigzagged lateral interactions	825:854	zigzagged lateral interactions	825:854	Micrometer-long mucin filaments emerge from microvillar-tips and, through zigzagged lateral interactions form a three-dimensional columnar network with a 30 nm mesh.
31925335	2	52	theme	nutrient	396:403	arg1	absorption					405:414	nutrient absorption	396:414	nutrient absorption	396:414	The intestinal epithelial glycocalyx, comprising glycosylated transmembrane mucins, is part of the primary host-microbe interface and is essential for nutrient absorption.
31925335	7	53	theme	single	1025:1030	arg1	plane					1032:1036	a single plane	1023:1036	a single plane	1023:1036	Filament-termini converge into globular structures ~30 nm apart that are liquid-crystalline packed within a single plane.
32345135	5	0	theme	composite	729:737	arg1	non-cytotoxic					755:767	non-cytotoxic	755:767	non-cytotoxic	755:767	All the prepared composite nanofibers were non-cytotoxic, able to support cell attachment, spread and proliferation.
32345135	5	0	theme	composite	729:737	arg1	nanofibers					739:748	All the prepared composite nanofibers	712:748	All the prepared composite nanofibers	712:748	All the prepared composite nanofibers were non-cytotoxic, able to support cell attachment, spread and proliferation.
32345135	6	1	theme	PLLA/gelatin/GAG	882:897	arg1	nanofibers					899:908	the PLLA/gelatin/GAG nanofibers	878:908	the PLLA/gelatin/GAG nanofibers	878:908	In the differentiation studies, the PLLA/GAG and the PLLA/gelatin/GAG nanofibers demonstrated stronger capacities in promoting the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers, even in the proliferation medium without extra inductive factors.
32345135	9	2	from	CS	1466:1467	arg1	ratio					1483:1487	1:1 weight ratio	1472:1487	1:1 weight ratio	1472:1487	It was found that the PLLA/gelatin/HA/CS (HA and CS in 1:1 weight ratio) nanofibers demonstrated a stronger synergetic effect on up-regulating chondrogenesis than both the PLLA/gelatin/HA and the PLLA/gelatin/CS nanofibers, when the GAG amounts in all the preparations were controlled as 3 wt.
32345135	3	3	theme	chondroitin	511:521	arg1	sulfate					523:529	chondroitin sulfate	511:529	chondroitin sulfate (CS)	511:534	However, it is not easy an task to electrospin glycosaminoglycan (GAG) like hyaluronic acid (HA) and chondroitin sulfate (CS) by themselves.
32345135	3	3	theme	chondroitin	511:521	arg1	CS					532:533	CS	532:533	CS	532:533	However, it is not easy an task to electrospin glycosaminoglycan (GAG) like hyaluronic acid (HA) and chondroitin sulfate (CS) by themselves.
32345135	6	4	theme	bone	1001:1004	arg1	marrow					1006:1011	the bone marrow	997:1011	the bone marrow mesenchymal stromal cells (BMSCs)	997:1045	In the differentiation studies, the PLLA/GAG and the PLLA/gelatin/GAG nanofibers demonstrated stronger capacities in promoting the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers, even in the proliferation medium without extra inductive factors.
32345135	4	5	from	co-electrospun	584:597	arg1	study					559:563	this study	554:563	this study	554:563	In this study, HA and/or CS were co-electrospun with poly(L-lactide) (PLLA) or PLLA/gelatin (1:1 in weight ratio) to obtain GAG-containing composite nanofibers.
32345135	4	6	theme	weight	651:656	arg1	ratio					658:662	weight ratio	651:662	weight ratio	651:662	In this study, HA and/or CS were co-electrospun with poly(L-lactide) (PLLA) or PLLA/gelatin (1:1 in weight ratio) to obtain GAG-containing composite nanofibers.
32345135	11	7	theme	GAG-containing	1721:1734	arg1	nanofibers					1746:1755	GAG-containing composite nanofibers	1721:1755	GAG-containing composite nanofibers	1721:1755	Herein, GAG-containing composite nanofibers were successfully electrospun and their potentials for cartilage repair were proved.
32345135	0	8	theme	cartilage	108:116	arg1	repair					118:123	cartilage repair	108:123	cartilage repair	108:123	Synthetic/natural blended polymer fibrous meshes composed of polylactide, gelatin and glycosaminoglycan for cartilage repair.
32345135	9	9	dep	nanofibers	1490:1499	arg1	demonstrated					1501:1512	demonstrated	1501:1512	demonstrated a stronger synergetic effect on up-regulating chondrogenesis than both the PLLA/gelatin/HA and the PLLA/gelatin/CS nanofibers	1501:1638	It was found that the PLLA/gelatin/HA/CS (HA and CS in 1:1 weight ratio) nanofibers demonstrated a stronger synergetic effect on up-regulating chondrogenesis than both the PLLA/gelatin/HA and the PLLA/gelatin/CS nanofibers, when the GAG amounts in all the preparations were controlled as 3 wt.
32345135	4	10	from	1:1	644:646	arg1	ratio					658:662	weight ratio	651:662	weight ratio	651:662	In this study, HA and/or CS were co-electrospun with poly(L-lactide) (PLLA) or PLLA/gelatin (1:1 in weight ratio) to obtain GAG-containing composite nanofibers.
32345135	11	11	theme	cartilage	1812:1820	arg1	repair					1822:1827	cartilage repair	1812:1827	cartilage repair	1812:1827	Herein, GAG-containing composite nanofibers were successfully electrospun and their potentials for cartilage repair were proved.
32345135	6	12	theme	extra	1159:1163	arg1	factors					1175:1181	extra inductive factors	1159:1181	extra inductive factors	1159:1181	In the differentiation studies, the PLLA/GAG and the PLLA/gelatin/GAG nanofibers demonstrated stronger capacities in promoting the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers, even in the proliferation medium without extra inductive factors.
32345135	8	13	theme	PLLA/GAG	1350:1357	arg1	nanofibers					1359:1368	the PLLA/GAG nanofibers	1346:1368	the PLLA/GAG nanofibers	1346:1368	At the meantime, the PLLA/gelatin/GAG nanofibers were more efficient than the PLLA/GAG nanofibers in enhancing the chondrogenic differentiation.
32345135	7	14	theme	fibrous	1256:1262	arg1	meshes					1264:1269	the fibrous meshes	1252:1269	the fibrous meshes	1252:1269	The incorporation of gelatin greatly improved the hydrophilicity of the fibrous meshes.
32345135	2	15	theme	natural	326:332	arg1	components					334:343	natural components	326:343	natural components derived from cartilage extracellular matrix (ECM)	326:393	As for cartilage regeneration, nanofibers containing natural components derived from cartilage extracellular matrix (ECM) are preferred.
32345135	9	16	theme	synergetic	1525:1534	arg1	effect					1536:1541	a stronger synergetic effect	1514:1541	a stronger synergetic effect on up-regulating chondrogenesis than both the PLLA/gelatin/HA and the PLLA/gelatin/CS nanofibers	1514:1638	It was found that the PLLA/gelatin/HA/CS (HA and CS in 1:1 weight ratio) nanofibers demonstrated a stronger synergetic effect on up-regulating chondrogenesis than both the PLLA/gelatin/HA and the PLLA/gelatin/CS nanofibers, when the GAG amounts in all the preparations were controlled as 3 wt.
32345135	8	17	from	meantime	1279:1286	arg1	nanofibers					1310:1319	the PLLA/gelatin/GAG nanofibers	1289:1319	the PLLA/gelatin/GAG nanofibers	1289:1319	At the meantime, the PLLA/gelatin/GAG nanofibers were more efficient than the PLLA/GAG nanofibers in enhancing the chondrogenic differentiation.
32345135	8	17	from	meantime	1279:1286	arg1	efficient					1331:1339	efficient	1331:1339	efficient	1331:1339	At the meantime, the PLLA/gelatin/GAG nanofibers were more efficient than the PLLA/GAG nanofibers in enhancing the chondrogenic differentiation.
32345135	6	18	theme	respective	1073:1082	arg1	PLLA					1084:1087	the respective PLLA and PLLA/gelatin nanofibers	1069:1115	PLLA	1084:1087	In the differentiation studies, the PLLA/GAG and the PLLA/gelatin/GAG nanofibers demonstrated stronger capacities in promoting the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers, even in the proliferation medium without extra inductive factors.
32345135	9	19	theme	weight	1476:1481	arg1	ratio					1483:1487	1:1 weight ratio	1472:1487	1:1 weight ratio	1472:1487	It was found that the PLLA/gelatin/HA/CS (HA and CS in 1:1 weight ratio) nanofibers demonstrated a stronger synergetic effect on up-regulating chondrogenesis than both the PLLA/gelatin/HA and the PLLA/gelatin/CS nanofibers, when the GAG amounts in all the preparations were controlled as 3 wt.
32345135	6	20	theme	inductive	1165:1173	arg1	factors					1175:1181	extra inductive factors	1159:1181	extra inductive factors	1159:1181	In the differentiation studies, the PLLA/GAG and the PLLA/gelatin/GAG nanofibers demonstrated stronger capacities in promoting the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers, even in the proliferation medium without extra inductive factors.
32345135	9	21	from	amounts	1654:1660	arg1	preparations					1673:1684	all the preparations	1665:1684	all the preparations	1665:1684	It was found that the PLLA/gelatin/HA/CS (HA and CS in 1:1 weight ratio) nanofibers demonstrated a stronger synergetic effect on up-regulating chondrogenesis than both the PLLA/gelatin/HA and the PLLA/gelatin/CS nanofibers, when the GAG amounts in all the preparations were controlled as 3 wt.
32345135	9	22	from	HA	1459:1460	arg1	ratio					1483:1487	1:1 weight ratio	1472:1487	1:1 weight ratio	1472:1487	It was found that the PLLA/gelatin/HA/CS (HA and CS in 1:1 weight ratio) nanofibers demonstrated a stronger synergetic effect on up-regulating chondrogenesis than both the PLLA/gelatin/HA and the PLLA/gelatin/CS nanofibers, when the GAG amounts in all the preparations were controlled as 3 wt.
32345135	2	23	theme	extracellular	368:380	arg1	ECM					390:392	ECM	390:392	ECM	390:392	As for cartilage regeneration, nanofibers containing natural components derived from cartilage extracellular matrix (ECM) are preferred.
32345135	2	23	theme	extracellular	368:380	arg1	matrix					382:387	cartilage extracellular matrix	358:387	cartilage extracellular matrix (ECM)	358:393	As for cartilage regeneration, nanofibers containing natural components derived from cartilage extracellular matrix (ECM) are preferred.
32345135	4	24	from	study	559:563	arg1	co-electrospun					584:597	co-electrospun	584:597	co-electrospun	584:597	In this study, HA and/or CS were co-electrospun with poly(L-lactide) (PLLA) or PLLA/gelatin (1:1 in weight ratio) to obtain GAG-containing composite nanofibers.
32345135	9	25	dep	PLLA/gelatin/HA/CS	1439:1456	arg1	CS					1466:1467	CS	1466:1467	CS	1466:1467	It was found that the PLLA/gelatin/HA/CS (HA and CS in 1:1 weight ratio) nanofibers demonstrated a stronger synergetic effect on up-regulating chondrogenesis than both the PLLA/gelatin/HA and the PLLA/gelatin/CS nanofibers, when the GAG amounts in all the preparations were controlled as 3 wt.
32345135	9	25	dep	PLLA/gelatin/HA/CS	1439:1456	arg1	HA					1459:1460	HA	1459:1460	HA	1459:1460	It was found that the PLLA/gelatin/HA/CS (HA and CS in 1:1 weight ratio) nanofibers demonstrated a stronger synergetic effect on up-regulating chondrogenesis than both the PLLA/gelatin/HA and the PLLA/gelatin/CS nanofibers, when the GAG amounts in all the preparations were controlled as 3 wt.
32345135	4	26	theme	composite	690:698	arg1	nanofibers					700:709	GAG-containing composite nanofibers	675:709	GAG-containing composite nanofibers	675:709	In this study, HA and/or CS were co-electrospun with poly(L-lactide) (PLLA) or PLLA/gelatin (1:1 in weight ratio) to obtain GAG-containing composite nanofibers.
32345135	6	27	theme	chondrocytes	1051:1062	arg1	differentiation					973:987	the chondrogenic differentiation	956:987	the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers	956:1115	In the differentiation studies, the PLLA/GAG and the PLLA/gelatin/GAG nanofibers demonstrated stronger capacities in promoting the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers, even in the proliferation medium without extra inductive factors.
32345135	0	28	theme	fibrous	34:40	arg1	meshes					42:47	polymer fibrous meshes	26:47	polymer fibrous meshes composed of polylactide, gelatin and glycosaminoglycan for cartilage repair	26:123	Synthetic/natural blended polymer fibrous meshes composed of polylactide, gelatin and glycosaminoglycan for cartilage repair.
32345135	2	29	contain	containing	315:324	arg1	nanofibers					304:313	nanofibers	304:313	nanofibers containing natural components derived from cartilage extracellular matrix (ECM)	304:393	As for cartilage regeneration, nanofibers containing natural components derived from cartilage extracellular matrix (ECM) are preferred.
32345135	2	29	contain	containing	315:324	arg2	components					334:343	natural components	326:343	natural components derived from cartilage extracellular matrix (ECM)	326:393	As for cartilage regeneration, nanofibers containing natural components derived from cartilage extracellular matrix (ECM) are preferred.
32345135	9	30	theme	GAG	1650:1652	arg1	3 wt					1705:1708	3 wt	1705:1708	3 wt	1705:1708	It was found that the PLLA/gelatin/HA/CS (HA and CS in 1:1 weight ratio) nanofibers demonstrated a stronger synergetic effect on up-regulating chondrogenesis than both the PLLA/gelatin/HA and the PLLA/gelatin/CS nanofibers, when the GAG amounts in all the preparations were controlled as 3 wt.
32345135	9	30	theme	GAG	1650:1652	arg1	amounts					1654:1660	the GAG amounts	1646:1660	the GAG amounts in all the preparations	1646:1684	It was found that the PLLA/gelatin/HA/CS (HA and CS in 1:1 weight ratio) nanofibers demonstrated a stronger synergetic effect on up-regulating chondrogenesis than both the PLLA/gelatin/HA and the PLLA/gelatin/CS nanofibers, when the GAG amounts in all the preparations were controlled as 3 wt.
32345135	9	31	theme	PLLA/gelatin/CS	1613:1627	arg1	nanofibers					1629:1638	both the PLLA/gelatin/HA and the PLLA/gelatin/CS nanofibers	1580:1638	nanofibers	1629:1638	It was found that the PLLA/gelatin/HA/CS (HA and CS in 1:1 weight ratio) nanofibers demonstrated a stronger synergetic effect on up-regulating chondrogenesis than both the PLLA/gelatin/HA and the PLLA/gelatin/CS nanofibers, when the GAG amounts in all the preparations were controlled as 3 wt.
32345135	3	32	theme	hyaluronic	486:495	arg1	HA					503:504	HA	503:504	HA	503:504	However, it is not easy an task to electrospin glycosaminoglycan (GAG) like hyaluronic acid (HA) and chondroitin sulfate (CS) by themselves.
32345135	3	32	theme	hyaluronic	486:495	arg1	acid					497:500	hyaluronic acid	486:500	hyaluronic acid (HA)	486:505	However, it is not easy an task to electrospin glycosaminoglycan (GAG) like hyaluronic acid (HA) and chondroitin sulfate (CS) by themselves.
32345135	2	33	theme	cartilage	358:366	arg1	ECM					390:392	ECM	390:392	ECM	390:392	As for cartilage regeneration, nanofibers containing natural components derived from cartilage extracellular matrix (ECM) are preferred.
32345135	2	33	theme	cartilage	358:366	arg1	matrix					382:387	cartilage extracellular matrix	358:387	cartilage extracellular matrix (ECM)	358:393	As for cartilage regeneration, nanofibers containing natural components derived from cartilage extracellular matrix (ECM) are preferred.
32345135	2	34	attach	derived	345:351	arg1	ECM					390:392	ECM	390:392	ECM	390:392	As for cartilage regeneration, nanofibers containing natural components derived from cartilage extracellular matrix (ECM) are preferred.
32345135	2	34	attach	derived	345:351	arg1	matrix					382:387	cartilage extracellular matrix	358:387	cartilage extracellular matrix (ECM)	358:393	As for cartilage regeneration, nanofibers containing natural components derived from cartilage extracellular matrix (ECM) are preferred.
32345135	2	34	attach	derived	345:351	arg2	components					334:343	natural components	326:343	natural components derived from cartilage extracellular matrix (ECM)	326:393	As for cartilage regeneration, nanofibers containing natural components derived from cartilage extracellular matrix (ECM) are preferred.
32345135	9	35	theme	stronger	1516:1523	arg1	effect					1536:1541	a stronger synergetic effect	1514:1541	a stronger synergetic effect on up-regulating chondrogenesis than both the PLLA/gelatin/HA and the PLLA/gelatin/CS nanofibers	1514:1638	It was found that the PLLA/gelatin/HA/CS (HA and CS in 1:1 weight ratio) nanofibers demonstrated a stronger synergetic effect on up-regulating chondrogenesis than both the PLLA/gelatin/HA and the PLLA/gelatin/CS nanofibers, when the GAG amounts in all the preparations were controlled as 3 wt.
32345135	6	36	theme	differentiation	836:850	arg1	studies					852:858	the differentiation studies	832:858	the differentiation studies	832:858	In the differentiation studies, the PLLA/GAG and the PLLA/gelatin/GAG nanofibers demonstrated stronger capacities in promoting the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers, even in the proliferation medium without extra inductive factors.
32345135	6	37	theme	proliferation	1130:1142	arg1	medium					1144:1149	the proliferation medium	1126:1149	the proliferation medium without extra inductive factors	1126:1181	In the differentiation studies, the PLLA/GAG and the PLLA/gelatin/GAG nanofibers demonstrated stronger capacities in promoting the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers, even in the proliferation medium without extra inductive factors.
32345135	1	38	theme	nanofibers	218:227	arg1	applications					259:270	biomimetic nanofibers targeting tissue regeneration applications	207:270	biomimetic nanofibers targeting tissue regeneration applications	207:270	Electrospinning is a common and effective technology used for the fabrication of biomimetic nanofibers targeting tissue regeneration applications.
32345135	11	39	theme	composite	1736:1744	arg1	nanofibers					1746:1755	GAG-containing composite nanofibers	1721:1755	GAG-containing composite nanofibers	1721:1755	Herein, GAG-containing composite nanofibers were successfully electrospun and their potentials for cartilage repair were proved.
32345135	9	40	theme	1:1	1472:1474	arg1	ratio					1483:1487	1:1 weight ratio	1472:1487	1:1 weight ratio	1472:1487	It was found that the PLLA/gelatin/HA/CS (HA and CS in 1:1 weight ratio) nanofibers demonstrated a stronger synergetic effect on up-regulating chondrogenesis than both the PLLA/gelatin/HA and the PLLA/gelatin/CS nanofibers, when the GAG amounts in all the preparations were controlled as 3 wt.
32345135	1	41	theme	targeting	229:237	arg1	applications					259:270	biomimetic nanofibers targeting tissue regeneration applications	207:270	biomimetic nanofibers targeting tissue regeneration applications	207:270	Electrospinning is a common and effective technology used for the fabrication of biomimetic nanofibers targeting tissue regeneration applications.
32345135	6	42	theme	cells	1033:1037	arg1	differentiation					973:987	the chondrogenic differentiation	956:987	the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers	956:1115	In the differentiation studies, the PLLA/GAG and the PLLA/gelatin/GAG nanofibers demonstrated stronger capacities in promoting the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers, even in the proliferation medium without extra inductive factors.
32345135	5	43	theme	cell	786:789	arg1	attachment					791:800	cell attachment	786:800	cell attachment	786:800	All the prepared composite nanofibers were non-cytotoxic, able to support cell attachment, spread and proliferation.
32345135	1	44	theme	tissue	239:244	arg1	applications					259:270	biomimetic nanofibers targeting tissue regeneration applications	207:270	biomimetic nanofibers targeting tissue regeneration applications	207:270	Electrospinning is a common and effective technology used for the fabrication of biomimetic nanofibers targeting tissue regeneration applications.
32345135	7	45	theme	gelatin	1205:1211	arg1	incorporation					1188:1200	The incorporation	1184:1200	The incorporation of gelatin	1184:1211	The incorporation of gelatin greatly improved the hydrophilicity of the fibrous meshes.
32345135	8	46	theme	chondrogenic	1387:1398	arg1	differentiation					1400:1414	the chondrogenic differentiation	1383:1414	the chondrogenic differentiation	1383:1414	At the meantime, the PLLA/gelatin/GAG nanofibers were more efficient than the PLLA/GAG nanofibers in enhancing the chondrogenic differentiation.
32345135	6	47	theme	chondrogenic	960:971	arg1	differentiation					973:987	the chondrogenic differentiation	956:987	the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers	956:1115	In the differentiation studies, the PLLA/GAG and the PLLA/gelatin/GAG nanofibers demonstrated stronger capacities in promoting the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers, even in the proliferation medium without extra inductive factors.
32345135	8	48	theme	PLLA/gelatin/GAG	1293:1308	arg1	nanofibers					1310:1319	the PLLA/gelatin/GAG nanofibers	1289:1319	the PLLA/gelatin/GAG nanofibers	1289:1319	At the meantime, the PLLA/gelatin/GAG nanofibers were more efficient than the PLLA/GAG nanofibers in enhancing the chondrogenic differentiation.
32345135	8	48	theme	PLLA/gelatin/GAG	1293:1308	arg1	efficient					1331:1339	efficient	1331:1339	efficient	1331:1339	At the meantime, the PLLA/gelatin/GAG nanofibers were more efficient than the PLLA/GAG nanofibers in enhancing the chondrogenic differentiation.
32345135	6	49	theme	stronger	923:930	arg1	capacities					932:941	stronger capacities	923:941	stronger capacities	923:941	In the differentiation studies, the PLLA/GAG and the PLLA/gelatin/GAG nanofibers demonstrated stronger capacities in promoting the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers, even in the proliferation medium without extra inductive factors.
32345135	4	50	with	co-electrospun	584:597	arg1	poly					604:607	poly	604:607	poly(L-lactide) (PLLA)	604:625	In this study, HA and/or CS were co-electrospun with poly(L-lactide) (PLLA) or PLLA/gelatin (1:1 in weight ratio) to obtain GAG-containing composite nanofibers.
32345135	4	50	with	co-electrospun	584:597	arg1	L-lactide					609:617	L-lactide	609:617	L-lactide	609:617	In this study, HA and/or CS were co-electrospun with poly(L-lactide) (PLLA) or PLLA/gelatin (1:1 in weight ratio) to obtain GAG-containing composite nanofibers.
32345135	4	50	with	co-electrospun	584:597	arg1	PLLA					621:624	PLLA	621:624	PLLA	621:624	In this study, HA and/or CS were co-electrospun with poly(L-lactide) (PLLA) or PLLA/gelatin (1:1 in weight ratio) to obtain GAG-containing composite nanofibers.
32345135	4	50	with	co-electrospun	584:597	arg1	PLLA/gelatin					630:641	PLLA/gelatin	630:641	PLLA/gelatin (1:1 in weight ratio)	630:663	In this study, HA and/or CS were co-electrospun with poly(L-lactide) (PLLA) or PLLA/gelatin (1:1 in weight ratio) to obtain GAG-containing composite nanofibers.
32345135	6	51	theme	stromal	1025:1031	arg1	BMSCs					1040:1044	BMSCs	1040:1044	BMSCs	1040:1044	In the differentiation studies, the PLLA/GAG and the PLLA/gelatin/GAG nanofibers demonstrated stronger capacities in promoting the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers, even in the proliferation medium without extra inductive factors.
32345135	6	51	theme	stromal	1025:1031	arg1	cells					1033:1037	the bone marrow mesenchymal stromal cells	997:1037	the bone marrow mesenchymal stromal cells (BMSCs)	997:1045	In the differentiation studies, the PLLA/GAG and the PLLA/gelatin/GAG nanofibers demonstrated stronger capacities in promoting the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers, even in the proliferation medium without extra inductive factors.
32345135	4	52	theme	GAG-containing	675:688	arg1	nanofibers					700:709	GAG-containing composite nanofibers	675:709	GAG-containing composite nanofibers	675:709	In this study, HA and/or CS were co-electrospun with poly(L-lactide) (PLLA) or PLLA/gelatin (1:1 in weight ratio) to obtain GAG-containing composite nanofibers.
32345135	1	53	theme	regeneration	246:257	arg1	applications					259:270	biomimetic nanofibers targeting tissue regeneration applications	207:270	biomimetic nanofibers targeting tissue regeneration applications	207:270	Electrospinning is a common and effective technology used for the fabrication of biomimetic nanofibers targeting tissue regeneration applications.
32345135	9	54	theme	PLLA/gelatin/HA	1589:1603	arg1	nanofibers					1629:1638	both the PLLA/gelatin/HA and the PLLA/gelatin/CS nanofibers	1580:1638	nanofibers	1629:1638	It was found that the PLLA/gelatin/HA/CS (HA and CS in 1:1 weight ratio) nanofibers demonstrated a stronger synergetic effect on up-regulating chondrogenesis than both the PLLA/gelatin/HA and the PLLA/gelatin/CS nanofibers, when the GAG amounts in all the preparations were controlled as 3 wt.
32345135	4	55	dep	PLLA/gelatin	630:641	arg1	1:1					644:646	1:1	644:646	1:1 in weight ratio	644:662	In this study, HA and/or CS were co-electrospun with poly(L-lactide) (PLLA) or PLLA/gelatin (1:1 in weight ratio) to obtain GAG-containing composite nanofibers.
32345135	1	56	theme	common	147:152	arg1	Electrospinning					126:140	Electrospinning	126:140	Electrospinning	126:140	Electrospinning is a common and effective technology used for the fabrication of biomimetic nanofibers targeting tissue regeneration applications.
32345135	1	56	theme	common	147:152	arg1	technology					168:177	a common and effective technology	145:177	a common and effective technology used for the fabrication of biomimetic nanofibers targeting tissue regeneration applications	145:270	Electrospinning is a common and effective technology used for the fabrication of biomimetic nanofibers targeting tissue regeneration applications.
32345135	1	57	theme	applications	259:270	arg1	fabrication					192:202	the fabrication	188:202	the fabrication of biomimetic nanofibers targeting tissue regeneration applications	188:270	Electrospinning is a common and effective technology used for the fabrication of biomimetic nanofibers targeting tissue regeneration applications.
32345135	6	58	theme	PLLA/gelatin	1093:1104	arg1	nanofibers					1106:1115	the respective PLLA and PLLA/gelatin nanofibers	1069:1115	nanofibers	1106:1115	In the differentiation studies, the PLLA/GAG and the PLLA/gelatin/GAG nanofibers demonstrated stronger capacities in promoting the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers, even in the proliferation medium without extra inductive factors.
32345135	2	59	theme	cartilage	280:288	arg1	regeneration					290:301	cartilage regeneration	280:301	cartilage regeneration	280:301	As for cartilage regeneration, nanofibers containing natural components derived from cartilage extracellular matrix (ECM) are preferred.
32345135	1	60	theme	biomimetic	207:216	arg1	applications					259:270	biomimetic nanofibers targeting tissue regeneration applications	207:270	biomimetic nanofibers targeting tissue regeneration applications	207:270	Electrospinning is a common and effective technology used for the fabrication of biomimetic nanofibers targeting tissue regeneration applications.
32345135	7	61	theme	meshes	1264:1269	arg1	hydrophilicity					1234:1247	the hydrophilicity	1230:1247	the hydrophilicity of the fibrous meshes	1230:1269	The incorporation of gelatin greatly improved the hydrophilicity of the fibrous meshes.
32345135	6	62	theme	mesenchymal	1013:1023	arg1	BMSCs					1040:1044	BMSCs	1040:1044	BMSCs	1040:1044	In the differentiation studies, the PLLA/GAG and the PLLA/gelatin/GAG nanofibers demonstrated stronger capacities in promoting the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers, even in the proliferation medium without extra inductive factors.
32345135	6	62	theme	mesenchymal	1013:1023	arg1	cells					1033:1037	the bone marrow mesenchymal stromal cells	997:1037	the bone marrow mesenchymal stromal cells (BMSCs)	997:1045	In the differentiation studies, the PLLA/GAG and the PLLA/gelatin/GAG nanofibers demonstrated stronger capacities in promoting the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers, even in the proliferation medium without extra inductive factors.
32345135	5	63	theme	prepared	720:727	arg1	non-cytotoxic					755:767	non-cytotoxic	755:767	non-cytotoxic	755:767	All the prepared composite nanofibers were non-cytotoxic, able to support cell attachment, spread and proliferation.
32345135	5	63	theme	prepared	720:727	arg1	nanofibers					739:748	All the prepared composite nanofibers	712:748	All the prepared composite nanofibers	712:748	All the prepared composite nanofibers were non-cytotoxic, able to support cell attachment, spread and proliferation.
32345135	0	64	theme	polymer	26:32	arg1	meshes					42:47	polymer fibrous meshes	26:47	polymer fibrous meshes composed of polylactide, gelatin and glycosaminoglycan for cartilage repair	26:123	Synthetic/natural blended polymer fibrous meshes composed of polylactide, gelatin and glycosaminoglycan for cartilage repair.
32345135	1	65	theme	effective	158:166	arg1	Electrospinning					126:140	Electrospinning	126:140	Electrospinning	126:140	Electrospinning is a common and effective technology used for the fabrication of biomimetic nanofibers targeting tissue regeneration applications.
32345135	1	65	theme	effective	158:166	arg1	technology					168:177	a common and effective technology	145:177	a common and effective technology used for the fabrication of biomimetic nanofibers targeting tissue regeneration applications	145:270	Electrospinning is a common and effective technology used for the fabrication of biomimetic nanofibers targeting tissue regeneration applications.
32345135	6	66	theme	marrow	1006:1011	arg1	BMSCs					1040:1044	BMSCs	1040:1044	BMSCs	1040:1044	In the differentiation studies, the PLLA/GAG and the PLLA/gelatin/GAG nanofibers demonstrated stronger capacities in promoting the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers, even in the proliferation medium without extra inductive factors.
32345135	6	66	theme	marrow	1006:1011	arg1	cells					1033:1037	the bone marrow mesenchymal stromal cells	997:1037	the bone marrow mesenchymal stromal cells (BMSCs)	997:1045	In the differentiation studies, the PLLA/GAG and the PLLA/gelatin/GAG nanofibers demonstrated stronger capacities in promoting the chondrogenic differentiation of both the bone marrow mesenchymal stromal cells (BMSCs) and chondrocytes than the respective PLLA and PLLA/gelatin nanofibers, even in the proliferation medium without extra inductive factors.
34649200	0	0	theme	green	123:127	arg1	applications					139:150	green packaging applications	123:150	green packaging applications	123:150	Rational formulations of sustainable polyurethane/chitin/rosin composites reinforced with ZnO-doped-SiO2 nanoparticles for green packaging applications.
34649200	3	1	theme	X-ray	738:742	arg1	XRD					757:759	XRD	757:759	XRD	757:759	The topology and surface roughness of chitin and nanoparticles within the TPU matrix, besides their effect on the crystallinity degree of TPU were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray diffraction (XRD).
34649200	3	1	theme	X-ray	738:742	arg1	diffraction					744:754	X-ray diffraction	738:754	X-ray diffraction (XRD)	738:760	The topology and surface roughness of chitin and nanoparticles within the TPU matrix, besides their effect on the crystallinity degree of TPU were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray diffraction (XRD).
34649200	1	2	theme	TPU	292:294	arg1	elastomer					297:305	the thermoplastic polyurethane (TPU) elastomer	260:305	the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite	260:334	Polysaccharide chitin (CH) was modified by antimicrobial natural gum rosin as a biocompatible agent within the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite.
34649200	3	3	theme	atomic	703:708	arg1	microscopy					716:725	atomic force microscopy	703:725	atomic force microscopy (AFM)	703:731	The topology and surface roughness of chitin and nanoparticles within the TPU matrix, besides their effect on the crystallinity degree of TPU were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray diffraction (XRD).
34649200	3	3	theme	atomic	703:708	arg1	AFM					728:730	AFM	728:730	AFM	728:730	The topology and surface roughness of chitin and nanoparticles within the TPU matrix, besides their effect on the crystallinity degree of TPU were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray diffraction (XRD).
34649200	6	4	theme	pathogenic	1258:1267	arg1	fungi					1269:1273	, pathogenic fungi	1256:1273	fungi	1269:1273	Furthermore, the TPU/CH blend reinforced with high contents of NPs (i.e., 5-7 wt%) exhibited efficient antimicrobial activities against Gram-negative, Gram-positive bacteria and, pathogenic fungi.
34649200	1	5	theme	antimicrobial	196:208	arg1	rosin					222:226	antimicrobial natural gum rosin	196:226	antimicrobial natural gum rosin	196:226	Polysaccharide chitin (CH) was modified by antimicrobial natural gum rosin as a biocompatible agent within the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite.
34649200	3	6	theme	force	710:714	arg1	microscopy					716:725	atomic force microscopy	703:725	atomic force microscopy (AFM)	703:731	The topology and surface roughness of chitin and nanoparticles within the TPU matrix, besides their effect on the crystallinity degree of TPU were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray diffraction (XRD).
34649200	3	6	theme	force	710:714	arg1	AFM					728:730	AFM	728:730	AFM	728:730	The topology and surface roughness of chitin and nanoparticles within the TPU matrix, besides their effect on the crystallinity degree of TPU were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray diffraction (XRD).
34649200	3	7	theme	TPU	577:579	arg1	matrix					581:586	the TPU matrix	573:586	the TPU matrix	573:586	The topology and surface roughness of chitin and nanoparticles within the TPU matrix, besides their effect on the crystallinity degree of TPU were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray diffraction (XRD).
34649200	5	8	theme	5 wt	1072:1075	arg1	%					1076:1076	5 wt%	1072:1076	5 wt%	1072:1076	The results showed that the major properties were improved when the nanoparticles were added, especially at 5 wt%.
34649200	0	9	theme	packaging	129:137	arg1	applications					139:150	green packaging applications	123:150	green packaging applications	123:150	Rational formulations of sustainable polyurethane/chitin/rosin composites reinforced with ZnO-doped-SiO2 nanoparticles for green packaging applications.
34649200	4	10	theme	antimicrobial	940:952	arg1	activity					954:961	antimicrobial activity	940:961	antimicrobial activity	940:961	The TPU nanocomposites are subjected to different measurements such as mechanical, thermal, hydrophobicity, flammability, water vapor, and oxygen barrier properties, as well as antimicrobial activity.
34649200	3	11	theme	surface	520:526	arg1	roughness					528:536	surface roughness	520:536	surface roughness	520:536	The topology and surface roughness of chitin and nanoparticles within the TPU matrix, besides their effect on the crystallinity degree of TPU were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray diffraction (XRD).
34649200	4	12	theme	TPU	767:769	arg1	nanocomposites					771:784	The TPU nanocomposites	763:784	The TPU nanocomposites	763:784	The TPU nanocomposites are subjected to different measurements such as mechanical, thermal, hydrophobicity, flammability, water vapor, and oxygen barrier properties, as well as antimicrobial activity.
34649200	1	13	theme	natural	210:216	arg1	rosin					222:226	antimicrobial natural gum rosin	196:226	antimicrobial natural gum rosin	196:226	Polysaccharide chitin (CH) was modified by antimicrobial natural gum rosin as a biocompatible agent within the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite.
34649200	4	14	theme	mechanical	834:843	arg1	hydrophobicity					855:868	hydrophobicity	855:868	hydrophobicity	855:868	The TPU nanocomposites are subjected to different measurements such as mechanical, thermal, hydrophobicity, flammability, water vapor, and oxygen barrier properties, as well as antimicrobial activity.
34649200	6	15	theme	NPs	1142:1144	arg1	contents					1130:1137	high contents	1125:1137	high contents of NPs (i.e., 5-7 wt%)	1125:1160	Furthermore, the TPU/CH blend reinforced with high contents of NPs (i.e., 5-7 wt%) exhibited efficient antimicrobial activities against Gram-negative, Gram-positive bacteria and, pathogenic fungi.
34649200	1	16	theme	gum	218:220	arg1	rosin					222:226	antimicrobial natural gum rosin	196:226	antimicrobial natural gum rosin	196:226	Polysaccharide chitin (CH) was modified by antimicrobial natural gum rosin as a biocompatible agent within the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite.
34649200	0	17	theme	Rational	0:7	arg1	formulations					9:20	Rational formulations	0:20	Rational formulations of sustainable polyurethane/chitin/rosin composites	0:72	Rational formulations of sustainable polyurethane/chitin/rosin composites reinforced with ZnO-doped-SiO2 nanoparticles for green packaging applications.
34649200	6	18	theme	Gram-negative	1215:1227	arg1	bacteria					1244:1251	Gram-negative, Gram-positive bacteria	1215:1251	Gram-negative, Gram-positive bacteria	1215:1251	Furthermore, the TPU/CH blend reinforced with high contents of NPs (i.e., 5-7 wt%) exhibited efficient antimicrobial activities against Gram-negative, Gram-positive bacteria and, pathogenic fungi.
34649200	0	19	theme	sustainable	25:35	arg1	composites					63:72	sustainable polyurethane/chitin/rosin composites	25:72	sustainable polyurethane/chitin/rosin composites	25:72	Rational formulations of sustainable polyurethane/chitin/rosin composites reinforced with ZnO-doped-SiO2 nanoparticles for green packaging applications.
34649200	4	20	theme	barrier	909:915	arg1	properties					917:926	oxygen barrier properties	902:926	oxygen barrier properties	902:926	The TPU nanocomposites are subjected to different measurements such as mechanical, thermal, hydrophobicity, flammability, water vapor, and oxygen barrier properties, as well as antimicrobial activity.
34649200	6	21	theme	high	1125:1128	arg1	contents					1130:1137	high contents	1125:1137	high contents of NPs (i.e., 5-7 wt%)	1125:1160	Furthermore, the TPU/CH blend reinforced with high contents of NPs (i.e., 5-7 wt%) exhibited efficient antimicrobial activities against Gram-negative, Gram-positive bacteria and, pathogenic fungi.
34649200	4	22	dep	mechanical	834:843	arg1	thermal					846:852	thermal	846:852	thermal	846:852	The TPU nanocomposites are subjected to different measurements such as mechanical, thermal, hydrophobicity, flammability, water vapor, and oxygen barrier properties, as well as antimicrobial activity.
34649200	1	23	theme	TPU/CH	319:324	arg1	composite					326:334	the TPU/CH composite	315:334	the TPU/CH composite	315:334	Polysaccharide chitin (CH) was modified by antimicrobial natural gum rosin as a biocompatible agent within the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite.
34649200	4	24	theme	oxygen	902:907	arg1	properties					917:926	oxygen barrier properties	902:926	oxygen barrier properties	902:926	The TPU nanocomposites are subjected to different measurements such as mechanical, thermal, hydrophobicity, flammability, water vapor, and oxygen barrier properties, as well as antimicrobial activity.
34649200	3	25	theme	electron	676:683	arg1	SEM					697:699	SEM	697:699	SEM	697:699	The topology and surface roughness of chitin and nanoparticles within the TPU matrix, besides their effect on the crystallinity degree of TPU were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray diffraction (XRD).
34649200	3	25	theme	electron	676:683	arg1	microscopy					685:694	scanning electron microscopy	667:694	scanning electron microscopy (SEM)	667:700	The topology and surface roughness of chitin and nanoparticles within the TPU matrix, besides their effect on the crystallinity degree of TPU were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray diffraction (XRD).
34649200	1	26	theme	Polysaccharide	153:166	arg1	CH					176:177	CH	176:177	CH	176:177	Polysaccharide chitin (CH) was modified by antimicrobial natural gum rosin as a biocompatible agent within the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite.
34649200	1	26	theme	Polysaccharide	153:166	arg1	chitin					168:173	Polysaccharide chitin	153:173	Polysaccharide chitin (CH)	153:178	Polysaccharide chitin (CH) was modified by antimicrobial natural gum rosin as a biocompatible agent within the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite.
34649200	1	26	theme	Polysaccharide	153:166	arg1	agent					247:251	a biocompatible agent	231:251	a biocompatible agent within the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite	231:334	Polysaccharide chitin (CH) was modified by antimicrobial natural gum rosin as a biocompatible agent within the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite.
34649200	1	27	theme	biocompatible	233:245	arg1	chitin					168:173	Polysaccharide chitin	153:173	Polysaccharide chitin (CH)	153:178	Polysaccharide chitin (CH) was modified by antimicrobial natural gum rosin as a biocompatible agent within the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite.
34649200	1	27	theme	biocompatible	233:245	arg1	agent					247:251	a biocompatible agent	231:251	a biocompatible agent within the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite	231:334	Polysaccharide chitin (CH) was modified by antimicrobial natural gum rosin as a biocompatible agent within the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite.
34649200	0	28	theme	composites	63:72	arg1	formulations					9:20	Rational formulations	0:20	Rational formulations of sustainable polyurethane/chitin/rosin composites	0:72	Rational formulations of sustainable polyurethane/chitin/rosin composites reinforced with ZnO-doped-SiO2 nanoparticles for green packaging applications.
34649200	3	29	theme	crystallinity	617:629	arg1	degree					631:636	the crystallinity degree	613:636	the crystallinity degree of TPU	613:643	The topology and surface roughness of chitin and nanoparticles within the TPU matrix, besides their effect on the crystallinity degree of TPU were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray diffraction (XRD).
34649200	1	30	mod	modified	184:191	arg1	CH					176:177	CH	176:177	CH	176:177	Polysaccharide chitin (CH) was modified by antimicrobial natural gum rosin as a biocompatible agent within the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite.
34649200	1	30	mod	modified	184:191	arg1	chitin					168:173	Polysaccharide chitin	153:173	Polysaccharide chitin (CH)	153:178	Polysaccharide chitin (CH) was modified by antimicrobial natural gum rosin as a biocompatible agent within the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite.
34649200	1	30	mod	modified	184:191	arg1	agent					247:251	a biocompatible agent	231:251	a biocompatible agent within the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite	231:334	Polysaccharide chitin (CH) was modified by antimicrobial natural gum rosin as a biocompatible agent within the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite.
34649200	1	30	mod	modified	184:191	arg3	rosin					222:226	antimicrobial natural gum rosin	196:226	antimicrobial natural gum rosin	196:226	Polysaccharide chitin (CH) was modified by antimicrobial natural gum rosin as a biocompatible agent within the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite.
34649200	0	31	theme	polyurethane/chitin/rosin	37:61	arg1	composites					63:72	sustainable polyurethane/chitin/rosin composites	25:72	sustainable polyurethane/chitin/rosin composites	25:72	Rational formulations of sustainable polyurethane/chitin/rosin composites reinforced with ZnO-doped-SiO2 nanoparticles for green packaging applications.
34649200	4	32	theme	different	803:811	arg1	properties					917:926	oxygen barrier properties	902:926	oxygen barrier properties	902:926	The TPU nanocomposites are subjected to different measurements such as mechanical, thermal, hydrophobicity, flammability, water vapor, and oxygen barrier properties, as well as antimicrobial activity.
34649200	4	32	theme	different	803:811	arg1	activity					954:961	antimicrobial activity	940:961	antimicrobial activity	940:961	The TPU nanocomposites are subjected to different measurements such as mechanical, thermal, hydrophobicity, flammability, water vapor, and oxygen barrier properties, as well as antimicrobial activity.
34649200	4	32	theme	different	803:811	arg1	vapor					891:895	water vapor	885:895	water vapor	885:895	The TPU nanocomposites are subjected to different measurements such as mechanical, thermal, hydrophobicity, flammability, water vapor, and oxygen barrier properties, as well as antimicrobial activity.
34649200	4	32	theme	different	803:811	arg1	measurements					813:824	different measurements	803:824	different measurements such as mechanical, thermal, hydrophobicity, flammability, water vapor, and oxygen barrier properties, as well as antimicrobial activity	803:961	The TPU nanocomposites are subjected to different measurements such as mechanical, thermal, hydrophobicity, flammability, water vapor, and oxygen barrier properties, as well as antimicrobial activity.
34649200	4	32	theme	different	803:811	arg1	hydrophobicity					855:868	hydrophobicity	855:868	hydrophobicity	855:868	The TPU nanocomposites are subjected to different measurements such as mechanical, thermal, hydrophobicity, flammability, water vapor, and oxygen barrier properties, as well as antimicrobial activity.
34649200	4	32	theme	different	803:811	arg1	flammability					871:882	flammability	871:882	flammability	871:882	The TPU nanocomposites are subjected to different measurements such as mechanical, thermal, hydrophobicity, flammability, water vapor, and oxygen barrier properties, as well as antimicrobial activity.
34649200	2	33	theme	nanocomposites	487:500	arg1	properties					469:478	the properties	465:478	the properties of TPU nanocomposites	465:500	This blend was then mixed with different ratios of ZnO-doped-SiO2 nanoparticles (ZnO-SiO2-NPs) to chelate chitin and to improve the properties of TPU nanocomposites.
34649200	3	34	theme	scanning	667:674	arg1	SEM					697:699	SEM	697:699	SEM	697:699	The topology and surface roughness of chitin and nanoparticles within the TPU matrix, besides their effect on the crystallinity degree of TPU were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray diffraction (XRD).
34649200	3	34	theme	scanning	667:674	arg1	microscopy					685:694	scanning electron microscopy	667:694	scanning electron microscopy (SEM)	667:700	The topology and surface roughness of chitin and nanoparticles within the TPU matrix, besides their effect on the crystallinity degree of TPU were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray diffraction (XRD).
34649200	6	35	theme	antimicrobial	1182:1194	arg1	activities					1196:1205	efficient antimicrobial activities	1172:1205	efficient antimicrobial activities against Gram-negative, Gram-positive bacteria and, pathogenic fungi	1172:1273	Furthermore, the TPU/CH blend reinforced with high contents of NPs (i.e., 5-7 wt%) exhibited efficient antimicrobial activities against Gram-negative, Gram-positive bacteria and, pathogenic fungi.
34649200	2	36	theme	different	368:376	arg1	ratios					378:383	different ratios	368:383	different ratios of ZnO-doped-SiO2 nanoparticles (ZnO-SiO2-NPs)	368:430	This blend was then mixed with different ratios of ZnO-doped-SiO2 nanoparticles (ZnO-SiO2-NPs) to chelate chitin and to improve the properties of TPU nanocomposites.
34649200	2	37	theme	TPU	483:485	arg1	nanocomposites					487:500	TPU nanocomposites	483:500	TPU nanocomposites	483:500	This blend was then mixed with different ratios of ZnO-doped-SiO2 nanoparticles (ZnO-SiO2-NPs) to chelate chitin and to improve the properties of TPU nanocomposites.
34649200	4	38	theme	water	885:889	arg1	vapor					891:895	water vapor	885:895	water vapor	885:895	The TPU nanocomposites are subjected to different measurements such as mechanical, thermal, hydrophobicity, flammability, water vapor, and oxygen barrier properties, as well as antimicrobial activity.
34649200	6	39	theme	efficient	1172:1180	arg1	activities					1196:1205	efficient antimicrobial activities	1172:1205	efficient antimicrobial activities against Gram-negative, Gram-positive bacteria and, pathogenic fungi	1172:1273	Furthermore, the TPU/CH blend reinforced with high contents of NPs (i.e., 5-7 wt%) exhibited efficient antimicrobial activities against Gram-negative, Gram-positive bacteria and, pathogenic fungi.
34649200	3	40	theme	chitin	541:546	arg1	topology					507:514	topology	507:514	topology	507:514	The topology and surface roughness of chitin and nanoparticles within the TPU matrix, besides their effect on the crystallinity degree of TPU were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray diffraction (XRD).
34649200	3	40	theme	chitin	541:546	arg1	roughness					528:536	surface roughness	520:536	surface roughness	520:536	The topology and surface roughness of chitin and nanoparticles within the TPU matrix, besides their effect on the crystallinity degree of TPU were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray diffraction (XRD).
34649200	5	41	theme	major	992:996	arg1	properties					998:1007	the major properties	988:1007	the major properties	988:1007	The results showed that the major properties were improved when the nanoparticles were added, especially at 5 wt%.
34649200	6	42	theme	TPU/CH	1096:1101	arg1	blend					1103:1107	the TPU/CH blend	1092:1107	the TPU/CH blend reinforced with high contents of NPs (i.e., 5-7 wt%)	1092:1160	Furthermore, the TPU/CH blend reinforced with high contents of NPs (i.e., 5-7 wt%) exhibited efficient antimicrobial activities against Gram-negative, Gram-positive bacteria and, pathogenic fungi.
34649200	6	43	dep	Gram-negative	1215:1227	arg1	Gram-positive					1230:1242	Gram-positive	1230:1242	Gram-positive	1230:1242	Furthermore, the TPU/CH blend reinforced with high contents of NPs (i.e., 5-7 wt%) exhibited efficient antimicrobial activities against Gram-negative, Gram-positive bacteria and, pathogenic fungi.
34649200	3	44	dep	topology	507:514	arg1	The					503:505	The	503:505	The	503:505	The topology and surface roughness of chitin and nanoparticles within the TPU matrix, besides their effect on the crystallinity degree of TPU were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray diffraction (XRD).
34649200	3	45	theme	nanoparticles	552:564	arg1	topology					507:514	topology	507:514	topology	507:514	The topology and surface roughness of chitin and nanoparticles within the TPU matrix, besides their effect on the crystallinity degree of TPU were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray diffraction (XRD).
34649200	3	45	theme	nanoparticles	552:564	arg1	roughness					528:536	surface roughness	520:536	surface roughness	520:536	The topology and surface roughness of chitin and nanoparticles within the TPU matrix, besides their effect on the crystallinity degree of TPU were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray diffraction (XRD).
34649200	1	46	theme	thermoplastic	264:276	arg1	elastomer					297:305	the thermoplastic polyurethane (TPU) elastomer	260:305	the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite	260:334	Polysaccharide chitin (CH) was modified by antimicrobial natural gum rosin as a biocompatible agent within the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite.
34649200	6	47	dep	%	1159:1159	arg1	i.e.					1147:1150	i.e.	1147:1150	i.e.	1147:1150	Furthermore, the TPU/CH blend reinforced with high contents of NPs (i.e., 5-7 wt%) exhibited efficient antimicrobial activities against Gram-negative, Gram-positive bacteria and, pathogenic fungi.
34649200	2	48	theme	nanoparticles	403:415	arg1	ratios					378:383	different ratios	368:383	different ratios of ZnO-doped-SiO2 nanoparticles (ZnO-SiO2-NPs)	368:430	This blend was then mixed with different ratios of ZnO-doped-SiO2 nanoparticles (ZnO-SiO2-NPs) to chelate chitin and to improve the properties of TPU nanocomposites.
34649200	3	49	from	effect	603:608	arg1	degree					631:636	the crystallinity degree	613:636	the crystallinity degree of TPU	613:643	The topology and surface roughness of chitin and nanoparticles within the TPU matrix, besides their effect on the crystallinity degree of TPU were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray diffraction (XRD).
34649200	1	50	theme	polyurethane	278:289	arg1	elastomer					297:305	the thermoplastic polyurethane (TPU) elastomer	260:305	the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite	260:334	Polysaccharide chitin (CH) was modified by antimicrobial natural gum rosin as a biocompatible agent within the thermoplastic polyurethane (TPU) elastomer to form the TPU/CH composite.
34649200	0	51	theme	ZnO-doped-SiO2	90:103	arg1	nanoparticles					105:117	ZnO-doped-SiO2 nanoparticles	90:117	ZnO-doped-SiO2 nanoparticles for green packaging applications	90:150	Rational formulations of sustainable polyurethane/chitin/rosin composites reinforced with ZnO-doped-SiO2 nanoparticles for green packaging applications.
34649200	3	52	theme	TPU	641:643	arg1	degree					631:636	the crystallinity degree	613:636	the crystallinity degree of TPU	613:643	The topology and surface roughness of chitin and nanoparticles within the TPU matrix, besides their effect on the crystallinity degree of TPU were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray diffraction (XRD).
34649200	2	53	theme	ZnO-doped-SiO2	388:401	arg1	ZnO-SiO2-NPs					418:429	ZnO-SiO2-NPs	418:429	ZnO-SiO2-NPs	418:429	This blend was then mixed with different ratios of ZnO-doped-SiO2 nanoparticles (ZnO-SiO2-NPs) to chelate chitin and to improve the properties of TPU nanocomposites.
34649200	2	53	theme	ZnO-doped-SiO2	388:401	arg1	nanoparticles					403:415	ZnO-doped-SiO2 nanoparticles	388:415	ZnO-doped-SiO2 nanoparticles (ZnO-SiO2-NPs)	388:430	This blend was then mixed with different ratios of ZnO-doped-SiO2 nanoparticles (ZnO-SiO2-NPs) to chelate chitin and to improve the properties of TPU nanocomposites.
31923485	3	0	theme	conversion	663:672	arg1	ratio					674:678	feed conversion ratio	658:678	feed conversion ratio	658:678	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	3	1	theme	glutamic	1040:1047	arg1	activity					1074:1081	glutamic oxaloacetic transaminase activity	1040:1081	glutamic oxaloacetic transaminase activity	1040:1081	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	2	2	contain	containing	518:527	arg2	EPP					552:554	20, 40, 60 and 80 g/kg EPP	529:554	20, 40, 60 and 80 g/kg EPP	529:554	Crucian carps with body weight of 51.24 ± 4.08 g were randomly divided into five groups: one group was fed with basic diet, while the other four groups were fed with diets containing 20, 40, 60 and 80 g/kg EPP.
31923485	2	2	contain	containing	518:527	arg1	diets					512:516	diets	512:516	diets containing 20, 40, 60 and 80 g/kg EPP	512:554	Crucian carps with body weight of 51.24 ± 4.08 g were randomly divided into five groups: one group was fed with basic diet, while the other four groups were fed with diets containing 20, 40, 60 and 80 g/kg EPP.
31923485	1	3	theme	present	162:168	arg1	study					170:174	The present study	158:174	The present study	158:174	The present study investigated dietary Enteromorpha prolifera polysaccharide (EPP) on the growth performance, body composition and non-specific immunity of crucian carp Carassius auratus.
31923485	0	4	theme	immunity	71:78	arg1	Modulation					0:9	Modulation	0:9	Modulation of the growth performance, body composition and nonspecific immunity of crucian carp Carassius auratus upon Enteromorpha prolifera polysaccharide.	0:156	Modulation of the growth performance, body composition and nonspecific immunity of crucian carp Carassius auratus upon Enteromorpha prolifera polysaccharide.
31923485	3	5	theme	oxaloacetic	1049:1059	arg1	activity					1074:1081	glutamic oxaloacetic transaminase activity	1040:1081	glutamic oxaloacetic transaminase activity	1040:1081	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	3	6	theme	alkaline	822:829	arg1	activity					843:850	alkaline phosphatase activity	822:850	alkaline phosphatase activity	822:850	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	1	7	theme	body	268:271	arg1	composition					273:283	body composition	268:283	body composition	268:283	The present study investigated dietary Enteromorpha prolifera polysaccharide (EPP) on the growth performance, body composition and non-specific immunity of crucian carp Carassius auratus.
31923485	0	8	theme	carp	91:94	arg1	immunity					71:78	nonspecific immunity	59:78	nonspecific immunity	59:78	Modulation of the growth performance, body composition and nonspecific immunity of crucian carp Carassius auratus upon Enteromorpha prolifera polysaccharide.
31923485	0	8	theme	carp	91:94	arg1	performance					25:35	growth performance	18:35	growth performance	18:35	Modulation of the growth performance, body composition and nonspecific immunity of crucian carp Carassius auratus upon Enteromorpha prolifera polysaccharide.
31923485	0	8	theme	carp	91:94	arg1	composition					43:53	body composition	38:53	body composition	38:53	Modulation of the growth performance, body composition and nonspecific immunity of crucian carp Carassius auratus upon Enteromorpha prolifera polysaccharide.
31923485	3	9	theme	EPP	617:619	arg1	administration					591:604	dietary administration	583:604	dietary administration of 40 g/kg EPP	583:619	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	3	10	theme	fat	944:946	arg1	content					948:954	body fat content	939:954	body fat content	939:954	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	3	11	theme	serum	770:774	arg1	activity					785:792	serum lysozyme activity	770:792	serum lysozyme activity	770:792	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	0	12	theme	crucian	83:89	arg1	carp					91:94	crucian carp	83:94	crucian carp	83:94	Modulation of the growth performance, body composition and nonspecific immunity of crucian carp Carassius auratus upon Enteromorpha prolifera polysaccharide.
31923485	3	13	theme	specific	681:688	arg1	rate					697:700	specific growth rate	681:700	specific growth rate	681:700	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	3	14	theme	serum	960:964	arg1	cholesterol					972:982	serum total cholesterol	960:982	serum total cholesterol	960:982	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	3	15	theme	lysozyme	776:783	arg1	activity					785:792	serum lysozyme activity	770:792	serum lysozyme activity	770:792	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	3	16	theme	phosphatase	831:841	arg1	activity					843:850	alkaline phosphatase activity	822:850	alkaline phosphatase activity	822:850	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	3	17	theme	growth	690:695	arg1	rate					697:700	specific growth rate	681:700	specific growth rate	681:700	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	3	18	theme	total	966:970	arg1	cholesterol					972:982	serum total cholesterol	960:982	serum total cholesterol	960:982	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	3	19	theme	digestive	741:749	arg1	activities					758:767	intestine digestive enzyme activities	731:767	intestine digestive enzyme activities	731:767	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	3	20	theme	alanine	1002:1008	arg1	activity					1027:1034	alanine aminotransferase activity	1002:1034	alanine aminotransferase activity	1002:1034	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	3	21	theme	complement	853:862	arg1	level					866:870	complement 3 level	853:870	complement 3 level	853:870	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	1	22	theme	dietary	189:195	arg1	EPP					236:238	EPP	236:238	EPP	236:238	The present study investigated dietary Enteromorpha prolifera polysaccharide (EPP) on the growth performance, body composition and non-specific immunity of crucian carp Carassius auratus.
31923485	1	22	theme	dietary	189:195	arg1	polysaccharide					220:233	dietary Enteromorpha prolifera polysaccharide	189:233	dietary Enteromorpha prolifera polysaccharide (EPP)	189:239	The present study investigated dietary Enteromorpha prolifera polysaccharide (EPP) on the growth performance, body composition and non-specific immunity of crucian carp Carassius auratus.
31923485	2	23	theme	basic	458:462	arg1	diet					464:467	basic diet	458:467	basic diet	458:467	Crucian carps with body weight of 51.24 ± 4.08 g were randomly divided into five groups: one group was fed with basic diet, while the other four groups were fed with diets containing 20, 40, 60 and 80 g/kg EPP.
31923485	2	24	theme	other	480:484	arg1	groups					491:496	the other four groups	476:496	the other four groups	476:496	Crucian carps with body weight of 51.24 ± 4.08 g were randomly divided into five groups: one group was fed with basic diet, while the other four groups were fed with diets containing 20, 40, 60 and 80 g/kg EPP.
31923485	3	25	theme	transaminase	1061:1072	arg1	activity					1074:1081	glutamic oxaloacetic transaminase activity	1040:1081	glutamic oxaloacetic transaminase activity	1040:1081	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	3	26	theme	aminotransferase	1010:1025	arg1	activity					1027:1034	alanine aminotransferase activity	1002:1034	alanine aminotransferase activity	1002:1034	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	1	27	theme	Enteromorpha	197:208	arg1	EPP					236:238	EPP	236:238	EPP	236:238	The present study investigated dietary Enteromorpha prolifera polysaccharide (EPP) on the growth performance, body composition and non-specific immunity of crucian carp Carassius auratus.
31923485	1	27	theme	Enteromorpha	197:208	arg1	polysaccharide					220:233	dietary Enteromorpha prolifera polysaccharide	189:233	dietary Enteromorpha prolifera polysaccharide (EPP)	189:239	The present study investigated dietary Enteromorpha prolifera polysaccharide (EPP) on the growth performance, body composition and non-specific immunity of crucian carp Carassius auratus.
31923485	1	28	theme	non-specific	289:300	arg1	immunity					302:309	non-specific immunity	289:309	non-specific immunity	289:309	The present study investigated dietary Enteromorpha prolifera polysaccharide (EPP) on the growth performance, body composition and non-specific immunity of crucian carp Carassius auratus.
31923485	3	29	theme	body	703:706	arg1	content					722:728	body crude protein content	703:728	body crude protein content	703:728	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	4	30	theme	control	1210:1216	arg1	group					1218:1222	the control group	1206:1222	the control group	1206:1222	All levels of EPP improved the resistance to Aeromonas hydrophilia compared with the control group.
31923485	5	31	theme	crucian	1284:1290	arg1	carp					1292:1295	crucian carp	1284:1295	crucian carp	1284:1295	The results indicated that EPP could promote the growth of crucian carp and improve their disease resistance and may be used as a dietary supplement for crucian carp.
31923485	1	32	theme	prolifera	210:218	arg1	EPP					236:238	EPP	236:238	EPP	236:238	The present study investigated dietary Enteromorpha prolifera polysaccharide (EPP) on the growth performance, body composition and non-specific immunity of crucian carp Carassius auratus.
31923485	1	32	theme	prolifera	210:218	arg1	polysaccharide					220:233	dietary Enteromorpha prolifera polysaccharide	189:233	dietary Enteromorpha prolifera polysaccharide (EPP)	189:239	The present study investigated dietary Enteromorpha prolifera polysaccharide (EPP) on the growth performance, body composition and non-specific immunity of crucian carp Carassius auratus.
31923485	0	33	theme	growth	18:23	arg1	performance					25:35	growth performance	18:35	growth performance	18:35	Modulation of the growth performance, body composition and nonspecific immunity of crucian carp Carassius auratus upon Enteromorpha prolifera polysaccharide.
31923485	3	34	theme	crude	708:712	arg1	content					722:728	body crude protein content	703:728	body crude protein content	703:728	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	0	35	theme	Enteromorpha	119:130	arg1	polysaccharide					142:155	Enteromorpha prolifera polysaccharide	119:155	Enteromorpha prolifera polysaccharide	119:155	Modulation of the growth performance, body composition and nonspecific immunity of crucian carp Carassius auratus upon Enteromorpha prolifera polysaccharide.
31923485	5	36	theme	carp	1292:1295	arg1	growth					1274:1279	the growth	1270:1279	the growth of crucian carp	1270:1295	The results indicated that EPP could promote the growth of crucian carp and improve their disease resistance and may be used as a dietary supplement for crucian carp.
31923485	3	37	theme	control	1110:1116	arg1	group					1118:1122	the control group	1106:1122	the control group	1106:1122	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	2	38	with	carps	354:358	arg1	weight					370:375	body weight	365:375	body weight of 51.24 ± 4.08 g	365:393	Crucian carps with body weight of 51.24 ± 4.08 g were randomly divided into five groups: one group was fed with basic diet, while the other four groups were fed with diets containing 20, 40, 60 and 80 g/kg EPP.
31923485	3	39	theme	superoxide	873:882	arg1	activity					894:901	superoxide dismutase activity	873:901	superoxide dismutase activity	873:901	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	1	40	theme	crucian	314:320	arg1	carp					322:325	crucian carp	314:325	crucian carp	314:325	The present study investigated dietary Enteromorpha prolifera polysaccharide (EPP) on the growth performance, body composition and non-specific immunity of crucian carp Carassius auratus.
31923485	1	41	dep	performance	255:265	arg1	the					244:246	the	244:246	the	244:246	The present study investigated dietary Enteromorpha prolifera polysaccharide (EPP) on the growth performance, body composition and non-specific immunity of crucian carp Carassius auratus.
31923485	3	42	theme	dismutase	884:892	arg1	activity					894:901	superoxide dismutase activity	873:901	superoxide dismutase activity	873:901	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	0	43	theme	performance	25:35	arg1	Modulation					0:9	Modulation	0:9	Modulation of the growth performance, body composition and nonspecific immunity of crucian carp Carassius auratus upon Enteromorpha prolifera polysaccharide.	0:156	Modulation of the growth performance, body composition and nonspecific immunity of crucian carp Carassius auratus upon Enteromorpha prolifera polysaccharide.
31923485	1	44	theme	carp	322:325	arg1	performance					255:265	growth performance	248:265	growth performance	248:265	The present study investigated dietary Enteromorpha prolifera polysaccharide (EPP) on the growth performance, body composition and non-specific immunity of crucian carp Carassius auratus.
31923485	1	44	theme	carp	322:325	arg1	composition					273:283	body composition	268:283	body composition	268:283	The present study investigated dietary Enteromorpha prolifera polysaccharide (EPP) on the growth performance, body composition and non-specific immunity of crucian carp Carassius auratus.
31923485	1	44	theme	carp	322:325	arg1	immunity					302:309	non-specific immunity	289:309	non-specific immunity	289:309	The present study investigated dietary Enteromorpha prolifera polysaccharide (EPP) on the growth performance, body composition and non-specific immunity of crucian carp Carassius auratus.
31923485	0	45	theme	prolifera	132:140	arg1	polysaccharide					142:155	Enteromorpha prolifera polysaccharide	119:155	Enteromorpha prolifera polysaccharide	119:155	Modulation of the growth performance, body composition and nonspecific immunity of crucian carp Carassius auratus upon Enteromorpha prolifera polysaccharide.
31923485	2	46	dep	groups	427:432	arg1	group					439:443	one group	435:443	five groups: one group was fed with basic diet, while the other four groups were fed with diets containing 20, 40, 60 and 80 g/kg EPP	422:554	Crucian carps with body weight of 51.24 ± 4.08 g were randomly divided into five groups: one group was fed with basic diet, while the other four groups were fed with diets containing 20, 40, 60 and 80 g/kg EPP.
31923485	0	47	theme	composition	43:53	arg1	Modulation					0:9	Modulation	0:9	Modulation of the growth performance, body composition and nonspecific immunity of crucian carp Carassius auratus upon Enteromorpha prolifera polysaccharide.	0:156	Modulation of the growth performance, body composition and nonspecific immunity of crucian carp Carassius auratus upon Enteromorpha prolifera polysaccharide.
31923485	3	48	theme	intestine	731:739	arg1	activities					758:767	intestine digestive enzyme activities	731:767	intestine digestive enzyme activities	731:767	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	3	49	theme	40 g/kg	609:615	arg1	EPP					617:619	40 g/kg EPP	609:619	40 g/kg EPP	609:619	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	5	50	theme	dietary	1355:1361	arg1	EPP					1252:1254	EPP	1252:1254	EPP	1252:1254	The results indicated that EPP could promote the growth of crucian carp and improve their disease resistance and may be used as a dietary supplement for crucian carp.
31923485	5	50	theme	dietary	1355:1361	arg1	supplement					1363:1372	a dietary supplement	1353:1372	a dietary supplement for crucian carp	1353:1389	The results indicated that EPP could promote the growth of crucian carp and improve their disease resistance and may be used as a dietary supplement for crucian carp.
31923485	2	51	theme	body	365:368	arg1	weight					370:375	body weight	365:375	body weight of 51.24 ± 4.08 g	365:393	Crucian carps with body weight of 51.24 ± 4.08 g were randomly divided into five groups: one group was fed with basic diet, while the other four groups were fed with diets containing 20, 40, 60 and 80 g/kg EPP.
31923485	0	52	theme	body	38:41	arg1	composition					43:53	body composition	38:53	body composition	38:53	Modulation of the growth performance, body composition and nonspecific immunity of crucian carp Carassius auratus upon Enteromorpha prolifera polysaccharide.
31923485	3	53	theme	protein	714:720	arg1	content					722:728	body crude protein content	703:728	body crude protein content	703:728	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	3	54	theme	weight	640:645	arg1	rate					652:655	the body weight gain rate	631:655	the body weight gain rate	631:655	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	0	55	dep	performance	25:35	arg1	the					14:16	the	14:16	the	14:16	Modulation of the growth performance, body composition and nonspecific immunity of crucian carp Carassius auratus upon Enteromorpha prolifera polysaccharide.
31923485	3	56	theme	catalase	907:914	arg1	activity					916:923	catalase activity	907:923	catalase activity	907:923	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	3	57	theme	gain	647:650	arg1	rate					652:655	the body weight gain rate	631:655	the body weight gain rate	631:655	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	3	58	theme	dietary	583:589	arg1	administration					591:604	dietary administration	583:604	dietary administration of 40 g/kg EPP	583:619	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	4	59	theme	Aeromonas	1170:1178	arg1	hydrophilia					1180:1190	Aeromonas hydrophilia	1170:1190	Aeromonas hydrophilia	1170:1190	All levels of EPP improved the resistance to Aeromonas hydrophilia compared with the control group.
31923485	5	60	used	used	1345:1348	arg2	supplement					1363:1372	a dietary supplement	1353:1372	a dietary supplement for crucian carp	1353:1389	The results indicated that EPP could promote the growth of crucian carp and improve their disease resistance and may be used as a dietary supplement for crucian carp.
31923485	5	60	used	used	1345:1348	arg2	EPP					1252:1254	EPP	1252:1254	EPP	1252:1254	The results indicated that EPP could promote the growth of crucian carp and improve their disease resistance and may be used as a dietary supplement for crucian carp.
31923485	5	61	theme	crucian	1378:1384	arg1	carp					1386:1389	crucian carp	1378:1389	crucian carp	1378:1389	The results indicated that EPP could promote the growth of crucian carp and improve their disease resistance and may be used as a dietary supplement for crucian carp.
31923485	4	62	theme	EPP	1139:1141	arg1	levels					1129:1134	All levels	1125:1134	All levels of EPP	1125:1141	All levels of EPP improved the resistance to Aeromonas hydrophilia compared with the control group.
31923485	2	63	theme	Crucian	346:352	arg1	carps					354:358	Crucian carps	346:358	Crucian carps with body weight of 51.24 ± 4.08 g	346:393	Crucian carps with body weight of 51.24 ± 4.08 g were randomly divided into five groups: one group was fed with basic diet, while the other four groups were fed with diets containing 20, 40, 60 and 80 g/kg EPP.
31923485	3	64	theme	acid	795:798	arg1	activity					812:819	acid phosphatase activity	795:819	acid phosphatase activity	795:819	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	0	65	theme	nonspecific	59:69	arg1	immunity					71:78	nonspecific immunity	59:78	nonspecific immunity	59:78	Modulation of the growth performance, body composition and nonspecific immunity of crucian carp Carassius auratus upon Enteromorpha prolifera polysaccharide.
31923485	3	66	theme	body	635:638	arg1	rate					652:655	the body weight gain rate	631:655	the body weight gain rate	631:655	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	3	67	theme	feeding	574:580	arg1	60 days					563:569	60 days	563:569	60 days of feeding	563:580	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	3	68	theme	phosphatase	800:810	arg1	activity					812:819	acid phosphatase activity	795:819	acid phosphatase activity	795:819	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	1	69	theme	growth	248:253	arg1	performance					255:265	growth performance	248:265	growth performance	248:265	The present study investigated dietary Enteromorpha prolifera polysaccharide (EPP) on the growth performance, body composition and non-specific immunity of crucian carp Carassius auratus.
31923485	5	70	theme	disease	1315:1321	arg1	resistance					1323:1332	their disease resistance	1309:1332	their disease resistance	1309:1332	The results indicated that EPP could promote the growth of crucian carp and improve their disease resistance and may be used as a dietary supplement for crucian carp.
31923485	3	71	theme	feed	658:661	arg1	ratio					674:678	feed conversion ratio	658:678	feed conversion ratio	658:678	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	3	72	theme	body	939:942	arg1	content					948:954	body fat content	939:954	body fat content	939:954	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
31923485	2	73	theme	51.24 ± 4.08 g	380:393	arg1	weight					370:375	body weight	365:375	body weight of 51.24 ± 4.08 g	365:393	Crucian carps with body weight of 51.24 ± 4.08 g were randomly divided into five groups: one group was fed with basic diet, while the other four groups were fed with diets containing 20, 40, 60 and 80 g/kg EPP.
31923485	3	74	theme	enzyme	751:756	arg1	activities					758:767	intestine digestive enzyme activities	731:767	intestine digestive enzyme activities	731:767	After 60 days of feeding, dietary administration of 40 g/kg EPP increased the body weight gain rate, feed conversion ratio, specific growth rate, body crude protein content, intestine digestive enzyme activities, serum lysozyme activity, acid phosphatase activity, alkaline phosphatase activity, complement 3 level, superoxide dismutase activity and catalase activity and decreased body fat content and serum total cholesterol, triacylglycerol, alanine aminotransferase activity and glutamic oxaloacetic transaminase activity compared with those of the control group.
34808775	5	0	theme	biopolymers	738:748	arg1	addition					726:733	the addition	722:733	the addition of biopolymers into alginate gels	722:767	The results showed that the addition of biopolymers into alginate gels increased the encapsulation efficiency (%EE) but reduced the release percentage of polyphenol in water and simulated gastric fluid (SGF).
34808775	1	1	theme	alginate	296:303	arg1	gels					305:308	alginate gels	296:308	alginate gels produced using spray aerosol technique	296:347	Effects of low methoxyl pectin, milk protein concentrate (MPC), and waxy starch on the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique were evaluated.
34808775	7	2	dep	%	1126:1126	arg1	to					1128:1129	to	1128:1129	to	1128:1129	It increased %EE from 63% to 68% in fresh gels, reduced the release percentage in water from 72% to 62% and reduced the release percentage in SGF from 76% to 67%.
34808775	3	3	theme	DSC	481:483	arg1	analysis					493:500	DSC thermal analysis	481:500	DSC thermal analysis	481:500	DSC thermal analysis and FTIR spectroscopy were used to prove the presence of polyphenols in gel matrixes.
34808775	0	4	theme	composite	67:75	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of alginate-biopolymers (protein, hydrocolloid, starch) composite	0:75	Evaluation of alginate-biopolymers (protein, hydrocolloid, starch) composite microgels prepared by the spray aerosol technique as a carrier for green tea polyphenols.
34808775	3	5	theme	thermal	485:491	arg1	analysis					493:500	DSC thermal analysis	481:500	DSC thermal analysis	481:500	DSC thermal analysis and FTIR spectroscopy were used to prove the presence of polyphenols in gel matrixes.
34808775	7	6	theme	72	1124:1125	arg1	%					1126:1126	%	1126:1126	%	1126:1126	It increased %EE from 63% to 68% in fresh gels, reduced the release percentage in water from 72% to 62% and reduced the release percentage in SGF from 76% to 67%.
34808775	1	7	from	encapsulation	254:266	arg1	gels					305:308	alginate gels	296:308	alginate gels produced using spray aerosol technique	296:347	Effects of low methoxyl pectin, milk protein concentrate (MPC), and waxy starch on the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique were evaluated.
34808775	5	8	from	percentage	838:847	arg1	water					866:870	water	866:870	water	866:870	The results showed that the addition of biopolymers into alginate gels increased the encapsulation efficiency (%EE) but reduced the release percentage of polyphenol in water and simulated gastric fluid (SGF).
34808775	5	8	from	percentage	838:847	arg1	SGF					901:903	SGF	901:903	SGF	901:903	The results showed that the addition of biopolymers into alginate gels increased the encapsulation efficiency (%EE) but reduced the release percentage of polyphenol in water and simulated gastric fluid (SGF).
34808775	5	8	from	percentage	838:847	arg1	fluid					894:898	simulated gastric fluid	876:898	simulated gastric fluid (SGF)	876:904	The results showed that the addition of biopolymers into alginate gels increased the encapsulation efficiency (%EE) but reduced the release percentage of polyphenol in water and simulated gastric fluid (SGF).
34808775	7	9	dep	%	1191:1191	arg1	to					1186:1187	to	1186:1187	to	1186:1187	It increased %EE from 63% to 68% in fresh gels, reduced the release percentage in water from 72% to 62% and reduced the release percentage in SGF from 76% to 67%.
34808775	3	10	attach	presence	547:554	arg1	matrixes					578:585	gel matrixes	574:585	gel matrixes	574:585	DSC thermal analysis and FTIR spectroscopy were used to prove the presence of polyphenols in gel matrixes.
34808775	3	10	attach	presence	547:554	arg2	polyphenols					559:569	polyphenols	559:569	polyphenols	559:569	DSC thermal analysis and FTIR spectroscopy were used to prove the presence of polyphenols in gel matrixes.
34808775	6	11	theme	cold-renneted	936:948	arg1	MPC					950:952	cold-renneted MPC	936:952	cold-renneted MPC	936:952	Among the three biopolymers, cold-renneted MPC gave the best protection for polyphenols encapsulated in alginate microgels.
34808775	0	12	theme	spray	103:107	arg1	technique					117:125	the spray aerosol technique	99:125	the spray aerosol technique	99:125	Evaluation of alginate-biopolymers (protein, hydrocolloid, starch) composite microgels prepared by the spray aerosol technique as a carrier for green tea polyphenols.
34808775	1	13	theme	spray	325:329	arg1	technique					339:347	spray aerosol technique	325:347	spray aerosol technique	325:347	Effects of low methoxyl pectin, milk protein concentrate (MPC), and waxy starch on the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique were evaluated.
34808775	7	14	theme	release	1091:1097	arg1	percentage					1099:1108	the release percentage	1087:1108	the release percentage	1087:1108	It increased %EE from 63% to 68% in fresh gels, reduced the release percentage in water from 72% to 62% and reduced the release percentage in SGF from 76% to 67%.
34808775	6	15	theme	alginate	1011:1018	arg1	microgels					1020:1028	alginate microgels	1011:1028	alginate microgels	1011:1028	Among the three biopolymers, cold-renneted MPC gave the best protection for polyphenols encapsulated in alginate microgels.
34808775	3	16	theme	FTIR	506:509	arg1	spectroscopy					511:522	FTIR spectroscopy	506:522	FTIR spectroscopy	506:522	DSC thermal analysis and FTIR spectroscopy were used to prove the presence of polyphenols in gel matrixes.
34808775	4	17	theme	polyphenols	631:641	arg1	release					643:649	the polyphenols release	627:649	the polyphenols release	627:649	The encapsulation efficiency (%EE) and the polyphenols release were investigated using Folin-Ciocalteu assay.
34808775	1	18	theme	aerosol	331:337	arg1	technique					339:347	spray aerosol technique	325:347	spray aerosol technique	325:347	Effects of low methoxyl pectin, milk protein concentrate (MPC), and waxy starch on the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique were evaluated.
34808775	5	19	theme	alginate	755:762	arg1	gels					764:767	alginate gels	755:767	alginate gels	755:767	The results showed that the addition of biopolymers into alginate gels increased the encapsulation efficiency (%EE) but reduced the release percentage of polyphenol in water and simulated gastric fluid (SGF).
34808775	7	20	theme	%	1044:1044	arg1	EE					1045:1046	%EE	1044:1046	%EE	1044:1046	It increased %EE from 63% to 68% in fresh gels, reduced the release percentage in water from 72% to 62% and reduced the release percentage in SGF from 76% to 67%.
34808775	5	21	theme	release	830:836	arg1	percentage					838:847	the release percentage	826:847	the release percentage of polyphenol in water and simulated gastric fluid (SGF)	826:904	The results showed that the addition of biopolymers into alginate gels increased the encapsulation efficiency (%EE) but reduced the release percentage of polyphenol in water and simulated gastric fluid (SGF).
34808775	2	22	theme	induced	416:422	arg1	method					433:438	cold-renneted induced gelation method	402:438	cold-renneted induced gelation method	402:438	MPC and waxy starch treated first by cold-renneted induced gelation method and gelatinization method, respectively.
34808775	7	23	theme	62	1131:1132	arg1	%					1126:1126	%	1126:1126	%	1126:1126	It increased %EE from 63% to 68% in fresh gels, reduced the release percentage in water from 72% to 62% and reduced the release percentage in SGF from 76% to 67%.
34808775	7	24	theme	%	1184:1184	arg1	%					1191:1191	76% to 67%	1182:1191	76% to 67%	1182:1191	It increased %EE from 63% to 68% in fresh gels, reduced the release percentage in water from 72% to 62% and reduced the release percentage in SGF from 76% to 67%.
34808775	0	25	dep	microgels	77:85	arg1	prepared					87:94	prepared	87:94	microgels prepared by the spray aerosol technique as a carrier for green tea polyphenols	77:164	Evaluation of alginate-biopolymers (protein, hydrocolloid, starch) composite microgels prepared by the spray aerosol technique as a carrier for green tea polyphenols.
34808775	3	26	used	used	529:532	arg2	analysis					493:500	DSC thermal analysis	481:500	DSC thermal analysis	481:500	DSC thermal analysis and FTIR spectroscopy were used to prove the presence of polyphenols in gel matrixes.
34808775	3	26	used	used	529:532	arg2	spectroscopy					511:522	FTIR spectroscopy	506:522	FTIR spectroscopy	506:522	DSC thermal analysis and FTIR spectroscopy were used to prove the presence of polyphenols in gel matrixes.
34808775	2	27	theme	cold-renneted	402:414	arg1	method					433:438	cold-renneted induced gelation method	402:438	cold-renneted induced gelation method	402:438	MPC and waxy starch treated first by cold-renneted induced gelation method and gelatinization method, respectively.
34808775	1	28	theme	waxy	235:238	arg1	starch					240:245	waxy starch	235:245	waxy starch	235:245	Effects of low methoxyl pectin, milk protein concentrate (MPC), and waxy starch on the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique were evaluated.
34808775	0	29	dep	composite	67:75	arg1	starch					59:64	alginate-biopolymers (protein, hydrocolloid, starch) composite	14:75	starch	59:64	Evaluation of alginate-biopolymers (protein, hydrocolloid, starch) composite microgels prepared by the spray aerosol technique as a carrier for green tea polyphenols.
34808775	0	29	dep	composite	67:75	arg1	hydrocolloid					45:56	alginate-biopolymers (protein, hydrocolloid, starch) composite	14:75	hydrocolloid	45:56	Evaluation of alginate-biopolymers (protein, hydrocolloid, starch) composite microgels prepared by the spray aerosol technique as a carrier for green tea polyphenols.
34808775	7	30	dep	%	1062:1062	arg1	to					1057:1058	to	1057:1058	to	1057:1058	It increased %EE from 63% to 68% in fresh gels, reduced the release percentage in water from 72% to 62% and reduced the release percentage in SGF from 76% to 67%.
34808775	1	31	theme	starch	240:245	arg1	Effects					167:173	Effects	167:173	Effects of low methoxyl pectin, milk protein concentrate (MPC), and waxy starch on the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique	167:347	Effects of low methoxyl pectin, milk protein concentrate (MPC), and waxy starch on the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique were evaluated.
34808775	7	32	from	%	1062:1062	arg1	gels					1073:1076	fresh gels	1067:1076	fresh gels	1067:1076	It increased %EE from 63% to 68% in fresh gels, reduced the release percentage in water from 72% to 62% and reduced the release percentage in SGF from 76% to 67%.
34808775	7	33	theme	%	1055:1055	arg1	%					1062:1062	63% to 68%	1053:1062	63% to 68% in fresh gels	1053:1076	It increased %EE from 63% to 68% in fresh gels, reduced the release percentage in water from 72% to 62% and reduced the release percentage in SGF from 76% to 67%.
34808775	1	34	theme	low	178:180	arg1	pectin					191:196	low methoxyl pectin	178:196	low methoxyl pectin	178:196	Effects of low methoxyl pectin, milk protein concentrate (MPC), and waxy starch on the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique were evaluated.
34808775	0	35	theme	alginate-biopolymers	14:33	arg1	composite					67:75	alginate-biopolymers (protein, hydrocolloid, starch) composite	14:75	composite	67:75	Evaluation of alginate-biopolymers (protein, hydrocolloid, starch) composite microgels prepared by the spray aerosol technique as a carrier for green tea polyphenols.
34808775	0	36	theme	aerosol	109:115	arg1	technique					117:125	the spray aerosol technique	99:125	the spray aerosol technique	99:125	Evaluation of alginate-biopolymers (protein, hydrocolloid, starch) composite microgels prepared by the spray aerosol technique as a carrier for green tea polyphenols.
34808775	1	37	theme	methoxyl	182:189	arg1	pectin					191:196	low methoxyl pectin	178:196	low methoxyl pectin	178:196	Effects of low methoxyl pectin, milk protein concentrate (MPC), and waxy starch on the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique were evaluated.
34808775	2	38	theme	gelatinization	444:457	arg1	method					459:464	gelatinization method	444:464	gelatinization method	444:464	MPC and waxy starch treated first by cold-renneted induced gelation method and gelatinization method, respectively.
34808775	5	39	theme	gastric	886:892	arg1	SGF					901:903	SGF	901:903	SGF	901:903	The results showed that the addition of biopolymers into alginate gels increased the encapsulation efficiency (%EE) but reduced the release percentage of polyphenol in water and simulated gastric fluid (SGF).
34808775	5	39	theme	gastric	886:892	arg1	fluid					894:898	simulated gastric fluid	876:898	simulated gastric fluid (SGF)	876:904	The results showed that the addition of biopolymers into alginate gels increased the encapsulation efficiency (%EE) but reduced the release percentage of polyphenol in water and simulated gastric fluid (SGF).
34808775	7	40	theme	release	1151:1157	arg1	percentage					1159:1168	the release percentage	1147:1168	the release percentage	1147:1168	It increased %EE from 63% to 68% in fresh gels, reduced the release percentage in water from 72% to 62% and reduced the release percentage in SGF from 76% to 67%.
34808775	1	41	theme	pectin	191:196	arg1	Effects					167:173	Effects	167:173	Effects of low methoxyl pectin, milk protein concentrate (MPC), and waxy starch on the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique	167:347	Effects of low methoxyl pectin, milk protein concentrate (MPC), and waxy starch on the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique were evaluated.
34808775	0	42	theme	protein	36:42	arg1	composite					67:75	alginate-biopolymers (protein, hydrocolloid, starch) composite	14:75	composite	67:75	Evaluation of alginate-biopolymers (protein, hydrocolloid, starch) composite microgels prepared by the spray aerosol technique as a carrier for green tea polyphenols.
34808775	3	43	theme	gel	574:576	arg1	matrixes					578:585	gel matrixes	574:585	gel matrixes	574:585	DSC thermal analysis and FTIR spectroscopy were used to prove the presence of polyphenols in gel matrixes.
34808775	4	44	theme	%	618:618	arg1	efficiency					606:615	The encapsulation efficiency	588:615	The encapsulation efficiency (%EE)	588:621	The encapsulation efficiency (%EE) and the polyphenols release were investigated using Folin-Ciocalteu assay.
34808775	4	44	theme	%	618:618	arg1	EE					619:620	%EE	618:620	%EE	618:620	The encapsulation efficiency (%EE) and the polyphenols release were investigated using Folin-Ciocalteu assay.
34808775	7	45	theme	fresh	1067:1071	arg1	gels					1073:1076	fresh gels	1067:1076	fresh gels	1067:1076	It increased %EE from 63% to 68% in fresh gels, reduced the release percentage in water from 72% to 62% and reduced the release percentage in SGF from 76% to 67%.
34808775	5	46	theme	simulated	876:884	arg1	SGF					901:903	SGF	901:903	SGF	901:903	The results showed that the addition of biopolymers into alginate gels increased the encapsulation efficiency (%EE) but reduced the release percentage of polyphenol in water and simulated gastric fluid (SGF).
34808775	5	46	theme	simulated	876:884	arg1	fluid					894:898	simulated gastric fluid	876:898	simulated gastric fluid (SGF)	876:904	The results showed that the addition of biopolymers into alginate gels increased the encapsulation efficiency (%EE) but reduced the release percentage of polyphenol in water and simulated gastric fluid (SGF).
34808775	2	47	theme	gelation	424:431	arg1	method					433:438	cold-renneted induced gelation method	402:438	cold-renneted induced gelation method	402:438	MPC and waxy starch treated first by cold-renneted induced gelation method and gelatinization method, respectively.
34808775	1	48	theme	green	271:275	arg1	tea-polyphenols					277:291	green tea-polyphenols	271:291	green tea-polyphenols	271:291	Effects of low methoxyl pectin, milk protein concentrate (MPC), and waxy starch on the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique were evaluated.
34808775	3	49	theme	polyphenols	559:569	arg1	presence					547:554	the presence	543:554	the presence of polyphenols in gel matrixes	543:585	DSC thermal analysis and FTIR spectroscopy were used to prove the presence of polyphenols in gel matrixes.
34808775	4	50	theme	Folin-Ciocalteu	675:689	arg1	assay					691:695	Folin-Ciocalteu assay	675:695	Folin-Ciocalteu assay	675:695	The encapsulation efficiency (%EE) and the polyphenols release were investigated using Folin-Ciocalteu assay.
34808775	1	51	theme	tea-polyphenols	277:291	arg1	encapsulation					254:266	the encapsulation	250:266	the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique	250:347	Effects of low methoxyl pectin, milk protein concentrate (MPC), and waxy starch on the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique were evaluated.
34808775	0	52	theme	tea	150:152	arg1	polyphenols					154:164	green tea polyphenols	144:164	green tea polyphenols	144:164	Evaluation of alginate-biopolymers (protein, hydrocolloid, starch) composite microgels prepared by the spray aerosol technique as a carrier for green tea polyphenols.
34808775	7	53	from	%	1191:1191	arg1	SGF					1173:1175	SGF	1173:1175	SGF from 76% to 67%	1173:1191	It increased %EE from 63% to 68% in fresh gels, reduced the release percentage in water from 72% to 62% and reduced the release percentage in SGF from 76% to 67%.
34808775	1	54	theme	milk	199:202	arg1	concentrate					212:222	milk protein concentrate	199:222	milk protein concentrate (MPC)	199:228	Effects of low methoxyl pectin, milk protein concentrate (MPC), and waxy starch on the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique were evaluated.
34808775	1	54	theme	milk	199:202	arg1	MPC					225:227	MPC	225:227	MPC	225:227	Effects of low methoxyl pectin, milk protein concentrate (MPC), and waxy starch on the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique were evaluated.
34808775	0	55	theme	green	144:148	arg1	polyphenols					154:164	green tea polyphenols	144:164	green tea polyphenols	144:164	Evaluation of alginate-biopolymers (protein, hydrocolloid, starch) composite microgels prepared by the spray aerosol technique as a carrier for green tea polyphenols.
34808775	7	56	from	%	1133:1133	arg1	water					1113:1117	water	1113:1117	water from 72% to 62%	1113:1133	It increased %EE from 63% to 68% in fresh gels, reduced the release percentage in water from 72% to 62% and reduced the release percentage in SGF from 76% to 67%.
34808775	1	57	from	Effects	167:173	arg1	encapsulation					254:266	the encapsulation	250:266	the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique	250:347	Effects of low methoxyl pectin, milk protein concentrate (MPC), and waxy starch on the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique were evaluated.
34808775	1	58	theme	protein	204:210	arg1	concentrate					212:222	milk protein concentrate	199:222	milk protein concentrate (MPC)	199:228	Effects of low methoxyl pectin, milk protein concentrate (MPC), and waxy starch on the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique were evaluated.
34808775	1	58	theme	protein	204:210	arg1	MPC					225:227	MPC	225:227	MPC	225:227	Effects of low methoxyl pectin, milk protein concentrate (MPC), and waxy starch on the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique were evaluated.
34808775	2	59	dep	MPC	365:367	arg1	starch					378:383	starch	378:383	starch	378:383	MPC and waxy starch treated first by cold-renneted induced gelation method and gelatinization method, respectively.
34808775	4	60	theme	encapsulation	592:604	arg1	efficiency					606:615	The encapsulation efficiency	588:615	The encapsulation efficiency (%EE)	588:621	The encapsulation efficiency (%EE) and the polyphenols release were investigated using Folin-Ciocalteu assay.
34808775	4	60	theme	encapsulation	592:604	arg1	EE					619:620	%EE	618:620	%EE	618:620	The encapsulation efficiency (%EE) and the polyphenols release were investigated using Folin-Ciocalteu assay.
34808775	5	61	theme	polyphenol	852:861	arg1	percentage					838:847	the release percentage	826:847	the release percentage of polyphenol in water and simulated gastric fluid (SGF)	826:904	The results showed that the addition of biopolymers into alginate gels increased the encapsulation efficiency (%EE) but reduced the release percentage of polyphenol in water and simulated gastric fluid (SGF).
34808775	3	62	from	presence	547:554	arg1	matrixes					578:585	gel matrixes	574:585	gel matrixes	574:585	DSC thermal analysis and FTIR spectroscopy were used to prove the presence of polyphenols in gel matrixes.
34808775	1	63	theme	concentrate	212:222	arg1	Effects					167:173	Effects	167:173	Effects of low methoxyl pectin, milk protein concentrate (MPC), and waxy starch on the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique	167:347	Effects of low methoxyl pectin, milk protein concentrate (MPC), and waxy starch on the encapsulation of green tea-polyphenols in alginate gels produced using spray aerosol technique were evaluated.
34808775	5	64	theme	%	809:809	arg1	efficiency					797:806	the encapsulation efficiency	779:806	the encapsulation efficiency (%EE)	779:812	The results showed that the addition of biopolymers into alginate gels increased the encapsulation efficiency (%EE) but reduced the release percentage of polyphenol in water and simulated gastric fluid (SGF).
34808775	5	64	theme	%	809:809	arg1	EE					810:811	%EE	809:811	%EE	809:811	The results showed that the addition of biopolymers into alginate gels increased the encapsulation efficiency (%EE) but reduced the release percentage of polyphenol in water and simulated gastric fluid (SGF).
34808775	5	65	theme	encapsulation	783:795	arg1	efficiency					797:806	the encapsulation efficiency	779:806	the encapsulation efficiency (%EE)	779:812	The results showed that the addition of biopolymers into alginate gels increased the encapsulation efficiency (%EE) but reduced the release percentage of polyphenol in water and simulated gastric fluid (SGF).
34808775	5	65	theme	encapsulation	783:795	arg1	EE					810:811	%EE	809:811	%EE	809:811	The results showed that the addition of biopolymers into alginate gels increased the encapsulation efficiency (%EE) but reduced the release percentage of polyphenol in water and simulated gastric fluid (SGF).
34808775	6	66	theme	best	963:966	arg1	protection					968:977	the best protection	959:977	the best protection for polyphenols encapsulated in alginate microgels	959:1028	Among the three biopolymers, cold-renneted MPC gave the best protection for polyphenols encapsulated in alginate microgels.
32531737	0	0	theme	flocs	86:90	arg1	exopolymers					50:60	alginate-like exopolymers	36:60	alginate-like exopolymers of aerobic granules and flocs	36:90	Chemical and physical properties of alginate-like exopolymers of aerobic granules and flocs produced from different wastewaters.
32531737	1	1	theme	Alginate-Like	322:334	arg1	Exopolymers					336:346	Alginate-Like Exopolymers	322:346	Alginate-Like Exopolymers (ALE)	322:352	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE) was studied.
32531737	4	2	theme	volatile	798:805	arg1	acids					813:817	no/very little volatile fatty acids	783:817	no/very little volatile fatty acids	783:817	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	1	3	theme	WW	158:159	arg1	composition					162:172	wastewater (WW) composition	146:172	wastewater (WW) composition	146:172	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE) was studied.
32531737	7	4	from	AGS	1004:1006	arg1	elasticity					988:997	ALE hydrogels elasticity	974:997	ALE hydrogels elasticity from AGS	974:1006	ALE hydrogels elasticity from AGS was always higher than from flocs when fed with real WW.
32531737	1	5	theme	Exopolymers	336:346	arg1	content					255:261	the content	251:261	the content	251:261	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE) was studied.
32531737	1	5	theme	Exopolymers	336:346	arg1	hydrogel					283:290	hydrogel	283:290	hydrogel	283:290	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE) was studied.
32531737	1	5	theme	Exopolymers	336:346	arg1	properties					308:317	rheological properties	296:317	rheological properties	296:317	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE) was studied.
32531737	4	6	theme	little	791:796	arg1	acids					813:817	no/very little volatile fatty acids	783:817	no/very little volatile fatty acids	783:817	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	3	7	theme	ALE	483:485	arg1	content					487:493	rather similar ALE content	468:493	rather similar ALE content	468:493	Overall, rather similar ALE content and composition was observed for the different types of sludge.
32531737	7	8	theme	real	1056:1059	arg1	WW					1061:1062	real WW	1056:1062	real WW	1056:1062	ALE hydrogels elasticity from AGS was always higher than from flocs when fed with real WW.
32531737	4	9	theme	uronic	691:696	arg1	sugars					698:703	uronic sugars	691:703	uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%)	691:753	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	10	theme	fatty	807:811	arg1	acids					813:817	no/very little volatile fatty acids	783:817	no/very little volatile fatty acids	783:817	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	11	from	ALE	708:710	arg1	amount					633:638	significantly larger amount	612:638	significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%)	612:753	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	11	from	ALE	708:710	arg1	sugars					698:703	uronic sugars	691:703	uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%)	691:753	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	11	from	ALE	708:710	arg1	ALE					643:645	ALE	643:645	ALE	643:645	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	3	12	theme	similar	475:481	arg1	content					487:493	rather similar ALE content	468:493	rather similar ALE content	468:493	Overall, rather similar ALE content and composition was observed for the different types of sludge.
32531737	4	13	theme	VSALE/g	660:666	arg1	VSsludge					668:675	261 ± 33 mg VSALE/g VSsludge	648:675	261 ± 33 mg VSALE/g VSsludge	648:675	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	13	theme	VSALE/g	660:666	arg1	%					681:681	+49%	678:681	+49%	678:681	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	1	14	theme	composition	162:172	arg1	influence					133:141	The influence	129:141	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE)	129:352	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE) was studied.
32531737	0	15	theme	different	106:114	arg1	wastewaters					116:126	different wastewaters	106:126	different wastewaters	106:126	Chemical and physical properties of alginate-like exopolymers of aerobic granules and flocs produced from different wastewaters.
32531737	2	16	theme	polysaccharides	442:456	arg1	mixture					405:411	a complex mixture	395:411	a complex mixture of proteins, humic acids and polysaccharides	395:456	Results showed that ALE are a complex mixture of proteins, humic acids and polysaccharides.
32531737	2	16	theme	polysaccharides	442:456	arg1	ALE					387:389	ALE	387:389	ALE	387:389	Results showed that ALE are a complex mixture of proteins, humic acids and polysaccharides.
32531737	1	17	from	influence	133:141	arg1	physical-chemical					264:280	physical-chemical	264:280	physical-chemical	264:280	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE) was studied.
32531737	1	17	from	influence	133:141	arg1	content					255:261	the content	251:261	the content	251:261	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE) was studied.
32531737	1	17	from	influence	133:141	arg1	hydrogel					283:290	hydrogel	283:290	hydrogel	283:290	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE) was studied.
32531737	1	17	from	influence	133:141	arg1	properties					308:317	rheological properties	296:317	rheological properties	296:317	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE) was studied.
32531737	2	18	theme	proteins	416:423	arg1	mixture					405:411	a complex mixture	395:411	a complex mixture of proteins, humic acids and polysaccharides	395:456	Results showed that ALE are a complex mixture of proteins, humic acids and polysaccharides.
32531737	2	18	theme	proteins	416:423	arg1	ALE					387:389	ALE	387:389	ALE	387:389	Results showed that ALE are a complex mixture of proteins, humic acids and polysaccharides.
32531737	4	19	from	amount	633:638	arg1	ALE					708:710	ALE	708:710	ALE	708:710	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	20	theme	larger	626:631	arg1	amount					633:638	significantly larger amount	612:638	significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%)	612:753	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	20	theme	larger	626:631	arg1	sugars					698:703	uronic sugars	691:703	uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%)	691:753	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	20	theme	larger	626:631	arg1	ALE					643:645	ALE	643:645	ALE	643:645	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	21	theme	no/very	783:789	arg1	acids					813:817	no/very little volatile fatty acids	783:817	no/very little volatile fatty acids	783:817	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	22	theme	ALE	643:645	arg1	amount					633:638	significantly larger amount	612:638	significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%)	612:753	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	22	theme	ALE	643:645	arg1	sugars					698:703	uronic sugars	691:703	uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%)	691:753	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	22	theme	ALE	643:645	arg1	ALE					643:645	ALE	643:645	ALE	643:645	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	0	23	theme	Chemical	0:7	arg1	properties					22:31	Chemical and physical properties	0:31	Chemical and physical properties of alginate-like exopolymers of aerobic granules and flocs	0:90	Chemical and physical properties of alginate-like exopolymers of aerobic granules and flocs produced from different wastewaters.
32531737	1	24	dep	Exopolymers	336:346	arg1	ALE					349:351	ALE	349:351	ALE	349:351	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE) was studied.
32531737	8	25	theme	different	1072:1080	arg1	types					1082:1086	different types	1072:1086	different types of sludge	1072:1096	Hence, different types of sludge impact the properties of the recovered ALE.
32531737	1	26	theme	bioaggregates	182:194	arg1	types					196:200	the bioaggregates types	178:200	the bioaggregates types (floccular vs. aerobic granular sludge - AGS)	178:246	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE) was studied.
32531737	4	27	dep	ALE	643:645	arg1	VSsludge					668:675	261 ± 33 mg VSALE/g VSsludge	648:675	261 ± 33 mg VSALE/g VSsludge	648:675	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	27	dep	ALE	643:645	arg1	%					681:681	+49%	678:681	+49%	678:681	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	28	theme	sugars	698:703	arg1	amount					633:638	significantly larger amount	612:638	significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%)	612:753	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	28	theme	sugars	698:703	arg1	sugars					698:703	uronic sugars	691:703	uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%)	691:753	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	28	theme	sugars	698:703	arg1	ALE					643:645	ALE	643:645	ALE	643:645	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	1	29	theme	types	196:200	arg1	influence					133:141	The influence	129:141	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE)	129:352	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE) was studied.
32531737	0	30	theme	physical	13:20	arg1	properties					22:31	Chemical and physical properties	0:31	Chemical and physical properties of alginate-like exopolymers of aerobic granules and flocs	0:90	Chemical and physical properties of alginate-like exopolymers of aerobic granules and flocs produced from different wastewaters.
32531737	6	31	theme	hydrogels	888:896	arg1	elasticity					898:907	ALE hydrogels elasticity	884:907	ALE hydrogels elasticity	884:907	ALE hydrogels elasticity changed significantly with the type/origin of the bioaggregates.
32531737	1	32	dep	sludge	234:239	arg1	AGS					243:245	AGS	243:245	floccular vs. aerobic granular sludge - AGS	203:245	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE) was studied.
32531737	3	33	theme	different	532:540	arg1	types					542:546	the different types	528:546	the different types of sludge	528:556	Overall, rather similar ALE content and composition was observed for the different types of sludge.
32531737	4	34	theme	261 ± 33 mg	648:658	arg1	VSsludge					668:675	261 ± 33 mg VSALE/g VSsludge	648:675	261 ± 33 mg VSALE/g VSsludge	648:675	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	34	theme	261 ± 33 mg	648:658	arg1	%					681:681	+49%	678:681	+49%	678:681	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	0	35	theme	alginate-like	36:48	arg1	exopolymers					50:60	alginate-like exopolymers	36:60	alginate-like exopolymers of aerobic granules and flocs	36:90	Chemical and physical properties of alginate-like exopolymers of aerobic granules and flocs produced from different wastewaters.
32531737	6	36	theme	ALE	884:886	arg1	elasticity					898:907	ALE hydrogels elasticity	884:907	ALE hydrogels elasticity	884:907	ALE hydrogels elasticity changed significantly with the type/origin of the bioaggregates.
32531737	4	37	dep	ALE	708:710	arg1	VSALE					742:746	254 ± 32 mgglucuronic acid/g VSALE	713:746	254 ± 32 mgglucuronic acid/g VSALE	713:746	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	37	dep	ALE	708:710	arg1	%					752:752	+62%	749:752	+62%	749:752	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	1	38	theme	floccular	203:211	arg1	sludge					234:239	floccular vs. aerobic granular sludge	203:239	floccular vs. aerobic granular sludge - AGS	203:245	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE) was studied.
32531737	8	39	theme	sludge	1091:1096	arg1	types					1082:1086	different types	1072:1086	different types of sludge	1072:1096	Hence, different types of sludge impact the properties of the recovered ALE.
32531737	2	40	theme	acids	432:436	arg1	mixture					405:411	a complex mixture	395:411	a complex mixture of proteins, humic acids and polysaccharides	395:456	Results showed that ALE are a complex mixture of proteins, humic acids and polysaccharides.
32531737	2	40	theme	acids	432:436	arg1	ALE					387:389	ALE	387:389	ALE	387:389	Results showed that ALE are a complex mixture of proteins, humic acids and polysaccharides.
32531737	3	41	theme	sludge	551:556	arg1	types					542:546	the different types	528:546	the different types of sludge	528:556	Overall, rather similar ALE content and composition was observed for the different types of sludge.
32531737	0	42	theme	exopolymers	50:60	arg1	properties					22:31	Chemical and physical properties	0:31	Chemical and physical properties of alginate-like exopolymers of aerobic granules and flocs	0:90	Chemical and physical properties of alginate-like exopolymers of aerobic granules and flocs produced from different wastewaters.
32531737	1	43	theme	rheological	296:306	arg1	properties					308:317	rheological properties	296:317	rheological properties	296:317	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE) was studied.
32531737	2	44	theme	humic	426:430	arg1	acids					432:436	humic acids	426:436	humic acids	426:436	Results showed that ALE are a complex mixture of proteins, humic acids and polysaccharides.
32531737	8	45	theme	ALE	1137:1139	arg1	properties					1109:1118	the properties	1105:1118	the properties of the recovered ALE	1105:1139	Hence, different types of sludge impact the properties of the recovered ALE.
32531737	7	46	theme	ALE	974:976	arg1	elasticity					988:997	ALE hydrogels elasticity	974:997	ALE hydrogels elasticity from AGS	974:1006	ALE hydrogels elasticity from AGS was always higher than from flocs when fed with real WW.
32531737	4	47	from	sugars	698:703	arg1	ALE					708:710	ALE	708:710	ALE	708:710	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	48	theme	acid/g	735:740	arg1	VSALE					742:746	254 ± 32 mgglucuronic acid/g VSALE	713:746	254 ± 32 mgglucuronic acid/g VSALE	713:746	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	48	theme	acid/g	735:740	arg1	%					752:752	+62%	749:752	+62%	749:752	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	6	49	theme	bioaggregates	959:971	arg1	type/origin					940:950	the type/origin	936:950	the type/origin of the bioaggregates	936:971	ALE hydrogels elasticity changed significantly with the type/origin of the bioaggregates.
32531737	5	50	theme	hydrogels	873:881	arg1	cohesion					857:864	the cohesion	853:864	the cohesion of the hydrogels	853:881	Mannuronic acids are involved in the cohesion of the hydrogels.
32531737	1	51	theme	aerobic	217:223	arg1	sludge					234:239	floccular vs. aerobic granular sludge	203:239	floccular vs. aerobic granular sludge - AGS	203:245	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE) was studied.
32531737	7	52	theme	hydrogels	978:986	arg1	elasticity					988:997	ALE hydrogels elasticity	974:997	ALE hydrogels elasticity from AGS	974:1006	ALE hydrogels elasticity from AGS was always higher than from flocs when fed with real WW.
32531737	4	53	theme	mgglucuronic	722:733	arg1	VSALE					742:746	254 ± 32 mgglucuronic acid/g VSALE	713:746	254 ± 32 mgglucuronic acid/g VSALE	713:746	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	4	53	theme	mgglucuronic	722:733	arg1	%					752:752	+62%	749:752	+62%	749:752	Only the AGS fed with acetate and propionate yielded significantly larger amount of ALE (261 ± 33 mg VSALE/g VSsludge, +49%) and of uronic sugars in ALE (254 ± 32 mgglucuronic acid/g VSALE, +62%) than bioaggregates fed with no/very little volatile fatty acids.
32531737	1	54	theme	granular	225:232	arg1	sludge					234:239	floccular vs. aerobic granular sludge	203:239	floccular vs. aerobic granular sludge - AGS	203:245	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE) was studied.
32531737	0	55	theme	granules	73:80	arg1	exopolymers					50:60	alginate-like exopolymers	36:60	alginate-like exopolymers of aerobic granules and flocs	36:90	Chemical and physical properties of alginate-like exopolymers of aerobic granules and flocs produced from different wastewaters.
32531737	1	56	dep	types	196:200	arg1	sludge					234:239	floccular vs. aerobic granular sludge	203:239	floccular vs. aerobic granular sludge - AGS	203:245	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE) was studied.
32531737	2	57	theme	complex	397:403	arg1	mixture					405:411	a complex mixture	395:411	a complex mixture of proteins, humic acids and polysaccharides	395:456	Results showed that ALE are a complex mixture of proteins, humic acids and polysaccharides.
32531737	2	57	theme	complex	397:403	arg1	ALE					387:389	ALE	387:389	ALE	387:389	Results showed that ALE are a complex mixture of proteins, humic acids and polysaccharides.
32531737	1	58	theme	wastewater	146:155	arg1	composition					162:172	wastewater (WW) composition	146:172	wastewater (WW) composition	146:172	The influence of wastewater (WW) composition and the bioaggregates types (floccular vs. aerobic granular sludge - AGS) on the content, physical-chemical, hydrogel and rheological properties of Alginate-Like Exopolymers (ALE) was studied.
32531737	0	59	theme	aerobic	65:71	arg1	granules					73:80	aerobic granules	65:80	aerobic granules	65:80	Chemical and physical properties of alginate-like exopolymers of aerobic granules and flocs produced from different wastewaters.
32531737	5	60	theme	Mannuronic	820:829	arg1	acids					831:835	Mannuronic acids	820:835	Mannuronic acids	820:835	Mannuronic acids are involved in the cohesion of the hydrogels.
34197039	2	0	theme	underlying	406:415	arg1	control					425:431	the underlying genetic control	402:431	the underlying genetic control of these traits	402:447	Some knowledge of the genetic factors that affect quality traits has been discovered through the study of classical maize mutants; however, much of the underlying genetic control of these traits and the interaction between these traits remains unknown.
34197039	3	1	theme	inbred	632:637	arg1	lines					639:643	501 diverse temperate maize inbred lines	604:643	501 diverse temperate maize inbred lines in five unique environments	604:671	To better understand variation that exists for grain compositional traits in maize, we evaluated 501 diverse temperate maize inbred lines in five unique environments and predicted 16 compositional traits (e.g., carbohydrates, protein, and starch) based on the output of near-infrared (NIR) spectroscopy.
34197039	6	2	theme	variation	1194:1202	arg1	%					1174:1174	22.9-71.0%	1165:1174	22.9-71.0% of the phenotypic variation across these traits	1165:1222	In total, 22.9-71.0% of the phenotypic variation across these traits could be explained using 2,386,666 single nucleotide polymorphism (SNP) markers generated from whole-genome resequencing data.
34197039	6	2	theme	variation	1194:1202	arg1	variation					1194:1202	the phenotypic variation	1179:1202	the phenotypic variation across these traits	1179:1222	In total, 22.9-71.0% of the phenotypic variation across these traits could be explained using 2,386,666 single nucleotide polymorphism (SNP) markers generated from whole-genome resequencing data.
34197039	1	3	theme	crop	121:124	arg1	worldwide					132:140	a multi-purpose row crop grown worldwide	101:140	a multi-purpose row crop grown worldwide	101:140	Maize (Zea mays L.) is a multi-purpose row crop grown worldwide, which, over time, has often been bred for increased yield at the detriment of lower composition grain quality.
34197039	1	3	theme	crop	121:124	arg1	Maize					78:82	Maize	78:82	Maize (Zea mays L.)	78:96	Maize (Zea mays L.) is a multi-purpose row crop grown worldwide, which, over time, has often been bred for increased yield at the detriment of lower composition grain quality.
34197039	1	4	theme	increased	185:193	arg1	yield					195:199	increased yield	185:199	increased yield at the detriment of lower composition grain quality	185:251	Maize (Zea mays L.) is a multi-purpose row crop grown worldwide, which, over time, has often been bred for increased yield at the detriment of lower composition grain quality.
34197039	0	5	from	control	8:14	arg1	panel					71:75	a maize diversity panel	53:75	a maize diversity panel	53:75	Genetic control of kernel compositional variation in a maize diversity panel.
34197039	6	6	theme	phenotypic	1183:1192	arg1	variation					1194:1202	the phenotypic variation	1179:1202	the phenotypic variation across these traits	1179:1222	In total, 22.9-71.0% of the phenotypic variation across these traits could be explained using 2,386,666 single nucleotide polymorphism (SNP) markers generated from whole-genome resequencing data.
34197039	8	7	dep	variation	1558:1566	arg1	the					1543:1545	the	1543:1545	the	1543:1545	This study provides valuable insights in the phenotypic variation and genetic control underlying compositional traits that can be used in breeding programs for improving maize grain quality.
34197039	1	8	theme	grown	126:130	arg1	worldwide					132:140	a multi-purpose row crop grown worldwide	101:140	a multi-purpose row crop grown worldwide	101:140	Maize (Zea mays L.) is a multi-purpose row crop grown worldwide, which, over time, has often been bred for increased yield at the detriment of lower composition grain quality.
34197039	1	8	theme	grown	126:130	arg1	Maize					78:82	Maize	78:82	Maize (Zea mays L.)	78:96	Maize (Zea mays L.) is a multi-purpose row crop grown worldwide, which, over time, has often been bred for increased yield at the detriment of lower composition grain quality.
34197039	7	9	theme	significant	1456:1466	arg1	SNPs					1468:1471	72 statistically significant SNPs	1439:1471	72 statistically significant SNPs	1439:1471	A genome-wide association study (GWAS) was conducted using these same markers and found 72 statistically significant SNPs for 11 compositional traits.
34197039	4	10	theme	compositional	863:875	arg1	traits					877:882	compositional traits	863:882	compositional traits	863:882	Phenotypic analysis found substantial variation for compositional traits and the majority of variation was explained by genetic and environmental factors.
34197039	3	11	theme	temperate	616:624	arg1	lines					639:643	501 diverse temperate maize inbred lines	604:643	501 diverse temperate maize inbred lines in five unique environments	604:671	To better understand variation that exists for grain compositional traits in maize, we evaluated 501 diverse temperate maize inbred lines in five unique environments and predicted 16 compositional traits (e.g., carbohydrates, protein, and starch) based on the output of near-infrared (NIR) spectroscopy.
34197039	2	12	theme	factors	284:290	arg1	knowledge					259:267	Some knowledge	254:267	Some knowledge of the genetic factors that affect quality traits	254:317	Some knowledge of the genetic factors that affect quality traits has been discovered through the study of classical maize mutants; however, much of the underlying genetic control of these traits and the interaction between these traits remains unknown.
34197039	7	13	theme	genome-wide	1353:1363	arg1	GWAS					1384:1387	GWAS	1384:1387	GWAS	1384:1387	A genome-wide association study (GWAS) was conducted using these same markers and found 72 statistically significant SNPs for 11 compositional traits.
34197039	7	13	theme	genome-wide	1353:1363	arg1	study					1377:1381	A genome-wide association study	1351:1381	A genome-wide association study (GWAS)	1351:1388	A genome-wide association study (GWAS) was conducted using these same markers and found 72 statistically significant SNPs for 11 compositional traits.
34197039	3	14	theme	near-infrared	777:789	arg1	spectroscopy					797:808	near-infrared (NIR) spectroscopy	777:808	near-infrared (NIR) spectroscopy	777:808	To better understand variation that exists for grain compositional traits in maize, we evaluated 501 diverse temperate maize inbred lines in five unique environments and predicted 16 compositional traits (e.g., carbohydrates, protein, and starch) based on the output of near-infrared (NIR) spectroscopy.
34197039	3	14	theme	near-infrared	777:789	arg1	NIR					792:794	NIR	792:794	NIR	792:794	To better understand variation that exists for grain compositional traits in maize, we evaluated 501 diverse temperate maize inbred lines in five unique environments and predicted 16 compositional traits (e.g., carbohydrates, protein, and starch) based on the output of near-infrared (NIR) spectroscopy.
34197039	5	15	theme	meaningful	1116:1125	arg1	correlations					1127:1138	meaningful correlations	1116:1138	meaningful correlations	1116:1138	Correlations and trade-offs among traits in different maize types (e.g., dent, sweetcorn, and popcorn) were explored, and significant differences and meaningful correlations were detected.
34197039	2	16	theme	traits	442:447	arg1	control					425:431	the underlying genetic control	402:431	the underlying genetic control of these traits	402:447	Some knowledge of the genetic factors that affect quality traits has been discovered through the study of classical maize mutants; however, much of the underlying genetic control of these traits and the interaction between these traits remains unknown.
34197039	2	16	theme	traits	442:447	arg1	interaction					457:467	the interaction	453:467	the interaction between these traits	453:488	Some knowledge of the genetic factors that affect quality traits has been discovered through the study of classical maize mutants; however, much of the underlying genetic control of these traits and the interaction between these traits remains unknown.
34197039	3	17	theme	diverse	608:614	arg1	lines					639:643	501 diverse temperate maize inbred lines	604:643	501 diverse temperate maize inbred lines in five unique environments	604:671	To better understand variation that exists for grain compositional traits in maize, we evaluated 501 diverse temperate maize inbred lines in five unique environments and predicted 16 compositional traits (e.g., carbohydrates, protein, and starch) based on the output of near-infrared (NIR) spectroscopy.
34197039	4	18	theme	environmental	943:955	arg1	factors					957:963	genetic and environmental factors	931:963	genetic and environmental factors	931:963	Phenotypic analysis found substantial variation for compositional traits and the majority of variation was explained by genetic and environmental factors.
34197039	7	19	theme	association	1365:1375	arg1	GWAS					1384:1387	GWAS	1384:1387	GWAS	1384:1387	A genome-wide association study (GWAS) was conducted using these same markers and found 72 statistically significant SNPs for 11 compositional traits.
34197039	7	19	theme	association	1365:1375	arg1	study					1377:1381	A genome-wide association study	1351:1381	A genome-wide association study (GWAS)	1351:1388	A genome-wide association study (GWAS) was conducted using these same markers and found 72 statistically significant SNPs for 11 compositional traits.
34197039	8	20	theme	breeding	1640:1647	arg1	programs					1649:1656	breeding programs	1640:1656	breeding programs	1640:1656	This study provides valuable insights in the phenotypic variation and genetic control underlying compositional traits that can be used in breeding programs for improving maize grain quality.
34197039	5	21	from	traits	1000:1005	arg1	types					1026:1030	different maize types	1010:1030	different maize types (e.g., dent, sweetcorn, and popcorn)	1010:1067	Correlations and trade-offs among traits in different maize types (e.g., dent, sweetcorn, and popcorn) were explored, and significant differences and meaningful correlations were detected.
34197039	3	22	theme	unique	653:658	arg1	environments					660:671	five unique environments	648:671	five unique environments	648:671	To better understand variation that exists for grain compositional traits in maize, we evaluated 501 diverse temperate maize inbred lines in five unique environments and predicted 16 compositional traits (e.g., carbohydrates, protein, and starch) based on the output of near-infrared (NIR) spectroscopy.
34197039	5	23	theme	maize	1020:1024	arg1	types					1026:1030	different maize types	1010:1030	different maize types (e.g., dent, sweetcorn, and popcorn)	1010:1067	Correlations and trade-offs among traits in different maize types (e.g., dent, sweetcorn, and popcorn) were explored, and significant differences and meaningful correlations were detected.
34197039	6	24	theme	single	1259:1264	arg1	polymorphism					1277:1288	2,386,666 single nucleotide polymorphism	1249:1288	2,386,666 single nucleotide polymorphism (SNP) markers generated from whole-genome resequencing data	1249:1348	In total, 22.9-71.0% of the phenotypic variation across these traits could be explained using 2,386,666 single nucleotide polymorphism (SNP) markers generated from whole-genome resequencing data.
34197039	6	24	theme	single	1259:1264	arg1	SNP					1291:1293	SNP	1291:1293	SNP	1291:1293	In total, 22.9-71.0% of the phenotypic variation across these traits could be explained using 2,386,666 single nucleotide polymorphism (SNP) markers generated from whole-genome resequencing data.
34197039	7	25	theme	compositional	1480:1492	arg1	traits					1494:1499	11 compositional traits	1477:1499	11 compositional traits	1477:1499	A genome-wide association study (GWAS) was conducted using these same markers and found 72 statistically significant SNPs for 11 compositional traits.
34197039	2	26	theme	mutants	376:382	arg1	study					351:355	the study	347:355	the study of classical maize mutants	347:382	Some knowledge of the genetic factors that affect quality traits has been discovered through the study of classical maize mutants; however, much of the underlying genetic control of these traits and the interaction between these traits remains unknown.
34197039	6	27	theme	nucleotide	1266:1275	arg1	polymorphism					1277:1288	2,386,666 single nucleotide polymorphism	1249:1288	2,386,666 single nucleotide polymorphism (SNP) markers generated from whole-genome resequencing data	1249:1348	In total, 22.9-71.0% of the phenotypic variation across these traits could be explained using 2,386,666 single nucleotide polymorphism (SNP) markers generated from whole-genome resequencing data.
34197039	6	27	theme	nucleotide	1266:1275	arg1	SNP					1291:1293	SNP	1291:1293	SNP	1291:1293	In total, 22.9-71.0% of the phenotypic variation across these traits could be explained using 2,386,666 single nucleotide polymorphism (SNP) markers generated from whole-genome resequencing data.
34197039	3	28	from	lines	639:643	arg1	environments					660:671	five unique environments	648:671	five unique environments	648:671	To better understand variation that exists for grain compositional traits in maize, we evaluated 501 diverse temperate maize inbred lines in five unique environments and predicted 16 compositional traits (e.g., carbohydrates, protein, and starch) based on the output of near-infrared (NIR) spectroscopy.
34197039	5	29	theme	significant	1088:1098	arg1	differences					1100:1110	significant differences	1088:1110	significant differences	1088:1110	Correlations and trade-offs among traits in different maize types (e.g., dent, sweetcorn, and popcorn) were explored, and significant differences and meaningful correlations were detected.
34197039	1	30	from	detriment	208:216	arg1	yield					195:199	increased yield	185:199	increased yield at the detriment of lower composition grain quality	185:251	Maize (Zea mays L.) is a multi-purpose row crop grown worldwide, which, over time, has often been bred for increased yield at the detriment of lower composition grain quality.
34197039	2	31	theme	control	425:431	arg1	much					394:397	much	394:397	much	394:397	Some knowledge of the genetic factors that affect quality traits has been discovered through the study of classical maize mutants; however, much of the underlying genetic control of these traits and the interaction between these traits remains unknown.
34197039	3	32	theme	spectroscopy	797:808	arg1	output					767:772	the output	763:772	the output of near-infrared (NIR) spectroscopy	763:808	To better understand variation that exists for grain compositional traits in maize, we evaluated 501 diverse temperate maize inbred lines in five unique environments and predicted 16 compositional traits (e.g., carbohydrates, protein, and starch) based on the output of near-infrared (NIR) spectroscopy.
34197039	6	33	theme	whole-genome	1319:1330	arg1	data					1345:1348	whole-genome resequencing data	1319:1348	whole-genome resequencing data	1319:1348	In total, 22.9-71.0% of the phenotypic variation across these traits could be explained using 2,386,666 single nucleotide polymorphism (SNP) markers generated from whole-genome resequencing data.
34197039	0	34	theme	Genetic	0:6	arg1	control					8:14	Genetic control	0:14	Genetic control of kernel compositional variation in a maize diversity panel.	0:76	Genetic control of kernel compositional variation in a maize diversity panel.
34197039	8	35	theme	maize	1672:1676	arg1	quality					1684:1690	maize grain quality	1672:1690	maize grain quality	1672:1690	This study provides valuable insights in the phenotypic variation and genetic control underlying compositional traits that can be used in breeding programs for improving maize grain quality.
34197039	0	36	theme	kernel	19:24	arg1	variation					40:48	kernel compositional variation	19:48	kernel compositional variation	19:48	Genetic control of kernel compositional variation in a maize diversity panel.
34197039	1	37	theme	Zea	85:87	arg1	L.					94:95	Zea mays L.	85:95	Zea mays L.	85:95	Maize (Zea mays L.) is a multi-purpose row crop grown worldwide, which, over time, has often been bred for increased yield at the detriment of lower composition grain quality.
34197039	1	37	theme	Zea	85:87	arg1	Maize					78:82	Maize	78:82	Maize (Zea mays L.)	78:96	Maize (Zea mays L.) is a multi-purpose row crop grown worldwide, which, over time, has often been bred for increased yield at the detriment of lower composition grain quality.
34197039	4	38	theme	variation	904:912	arg1	majority					892:899	the majority	888:899	the majority of variation	888:912	Phenotypic analysis found substantial variation for compositional traits and the majority of variation was explained by genetic and environmental factors.
34197039	2	39	theme	classical	360:368	arg1	mutants					376:382	classical maize mutants	360:382	classical maize mutants	360:382	Some knowledge of the genetic factors that affect quality traits has been discovered through the study of classical maize mutants; however, much of the underlying genetic control of these traits and the interaction between these traits remains unknown.
34197039	1	40	theme	lower	221:225	arg1	quality					245:251	lower composition grain quality	221:251	lower composition grain quality	221:251	Maize (Zea mays L.) is a multi-purpose row crop grown worldwide, which, over time, has often been bred for increased yield at the detriment of lower composition grain quality.
34197039	3	41	dep	traits	704:709	arg1	protein					733:739	protein	733:739	protein	733:739	To better understand variation that exists for grain compositional traits in maize, we evaluated 501 diverse temperate maize inbred lines in five unique environments and predicted 16 compositional traits (e.g., carbohydrates, protein, and starch) based on the output of near-infrared (NIR) spectroscopy.
34197039	3	41	dep	traits	704:709	arg1	starch					746:751	starch	746:751	starch	746:751	To better understand variation that exists for grain compositional traits in maize, we evaluated 501 diverse temperate maize inbred lines in five unique environments and predicted 16 compositional traits (e.g., carbohydrates, protein, and starch) based on the output of near-infrared (NIR) spectroscopy.
34197039	3	41	dep	traits	704:709	arg1	carbohydrates					718:730	carbohydrates	718:730	carbohydrates	718:730	To better understand variation that exists for grain compositional traits in maize, we evaluated 501 diverse temperate maize inbred lines in five unique environments and predicted 16 compositional traits (e.g., carbohydrates, protein, and starch) based on the output of near-infrared (NIR) spectroscopy.
34197039	6	42	theme	resequencing	1332:1343	arg1	data					1345:1348	whole-genome resequencing data	1319:1348	whole-genome resequencing data	1319:1348	In total, 22.9-71.0% of the phenotypic variation across these traits could be explained using 2,386,666 single nucleotide polymorphism (SNP) markers generated from whole-genome resequencing data.
34197039	1	43	theme	mays	89:92	arg1	L.					94:95	Zea mays L.	85:95	Zea mays L.	85:95	Maize (Zea mays L.) is a multi-purpose row crop grown worldwide, which, over time, has often been bred for increased yield at the detriment of lower composition grain quality.
34197039	1	43	theme	mays	89:92	arg1	Maize					78:82	Maize	78:82	Maize (Zea mays L.)	78:96	Maize (Zea mays L.) is a multi-purpose row crop grown worldwide, which, over time, has often been bred for increased yield at the detriment of lower composition grain quality.
34197039	5	44	dep	dent	1039:1042	arg1	e.g.					1033:1036	e.g.	1033:1036	e.g.	1033:1036	Correlations and trade-offs among traits in different maize types (e.g., dent, sweetcorn, and popcorn) were explored, and significant differences and meaningful correlations were detected.
34197039	1	45	theme	multi-purpose	103:115	arg1	worldwide					132:140	a multi-purpose row crop grown worldwide	101:140	a multi-purpose row crop grown worldwide	101:140	Maize (Zea mays L.) is a multi-purpose row crop grown worldwide, which, over time, has often been bred for increased yield at the detriment of lower composition grain quality.
34197039	1	45	theme	multi-purpose	103:115	arg1	Maize					78:82	Maize	78:82	Maize (Zea mays L.)	78:96	Maize (Zea mays L.) is a multi-purpose row crop grown worldwide, which, over time, has often been bred for increased yield at the detriment of lower composition grain quality.
34197039	0	46	theme	variation	40:48	arg1	control					8:14	Genetic control	0:14	Genetic control of kernel compositional variation in a maize diversity panel.	0:76	Genetic control of kernel compositional variation in a maize diversity panel.
34197039	1	47	theme	composition	227:237	arg1	quality					245:251	lower composition grain quality	221:251	lower composition grain quality	221:251	Maize (Zea mays L.) is a multi-purpose row crop grown worldwide, which, over time, has often been bred for increased yield at the detriment of lower composition grain quality.
34197039	2	48	theme	interaction	457:467	arg1	much					394:397	much	394:397	much	394:397	Some knowledge of the genetic factors that affect quality traits has been discovered through the study of classical maize mutants; however, much of the underlying genetic control of these traits and the interaction between these traits remains unknown.
34197039	3	49	dep	carbohydrates	718:730	arg1	e.g.					712:715	e.g.	712:715	e.g.	712:715	To better understand variation that exists for grain compositional traits in maize, we evaluated 501 diverse temperate maize inbred lines in five unique environments and predicted 16 compositional traits (e.g., carbohydrates, protein, and starch) based on the output of near-infrared (NIR) spectroscopy.
34197039	8	50	theme	grain	1678:1682	arg1	quality					1684:1690	maize grain quality	1672:1690	maize grain quality	1672:1690	This study provides valuable insights in the phenotypic variation and genetic control underlying compositional traits that can be used in breeding programs for improving maize grain quality.
34197039	0	51	theme	compositional	26:38	arg1	variation					40:48	kernel compositional variation	19:48	kernel compositional variation	19:48	Genetic control of kernel compositional variation in a maize diversity panel.
34197039	1	52	theme	grain	239:243	arg1	quality					245:251	lower composition grain quality	221:251	lower composition grain quality	221:251	Maize (Zea mays L.) is a multi-purpose row crop grown worldwide, which, over time, has often been bred for increased yield at the detriment of lower composition grain quality.
34197039	4	53	theme	substantial	837:847	arg1	variation					849:857	substantial variation	837:857	substantial variation for compositional traits	837:882	Phenotypic analysis found substantial variation for compositional traits and the majority of variation was explained by genetic and environmental factors.
34197039	3	54	theme	compositional	560:572	arg1	traits					574:579	grain compositional traits	554:579	grain compositional traits in maize	554:588	To better understand variation that exists for grain compositional traits in maize, we evaluated 501 diverse temperate maize inbred lines in five unique environments and predicted 16 compositional traits (e.g., carbohydrates, protein, and starch) based on the output of near-infrared (NIR) spectroscopy.
34197039	2	55	theme	maize	370:374	arg1	mutants					376:382	classical maize mutants	360:382	classical maize mutants	360:382	Some knowledge of the genetic factors that affect quality traits has been discovered through the study of classical maize mutants; however, much of the underlying genetic control of these traits and the interaction between these traits remains unknown.
34197039	4	56	theme	genetic	931:937	arg1	factors					957:963	genetic and environmental factors	931:963	genetic and environmental factors	931:963	Phenotypic analysis found substantial variation for compositional traits and the majority of variation was explained by genetic and environmental factors.
34197039	1	57	theme	quality	245:251	arg1	detriment					208:216	the detriment	204:216	the detriment of lower composition grain quality	204:251	Maize (Zea mays L.) is a multi-purpose row crop grown worldwide, which, over time, has often been bred for increased yield at the detriment of lower composition grain quality.
34197039	2	58	theme	genetic	417:423	arg1	control					425:431	the underlying genetic control	402:431	the underlying genetic control of these traits	402:447	Some knowledge of the genetic factors that affect quality traits has been discovered through the study of classical maize mutants; however, much of the underlying genetic control of these traits and the interaction between these traits remains unknown.
34197039	2	59	theme	genetic	276:282	arg1	factors					284:290	the genetic factors	272:290	the genetic factors that affect quality traits	272:317	Some knowledge of the genetic factors that affect quality traits has been discovered through the study of classical maize mutants; however, much of the underlying genetic control of these traits and the interaction between these traits remains unknown.
34197039	8	60	from	insights	1531:1538	arg1	variation					1558:1566	phenotypic variation	1547:1566	phenotypic variation	1547:1566	This study provides valuable insights in the phenotypic variation and genetic control underlying compositional traits that can be used in breeding programs for improving maize grain quality.
34197039	8	60	from	insights	1531:1538	arg1	control					1580:1586	genetic control	1572:1586	genetic control	1572:1586	This study provides valuable insights in the phenotypic variation and genetic control underlying compositional traits that can be used in breeding programs for improving maize grain quality.
34197039	3	61	theme	compositional	690:702	arg1	traits					704:709	16 compositional traits	687:709	16 compositional traits (e.g., carbohydrates, protein, and starch)	687:752	To better understand variation that exists for grain compositional traits in maize, we evaluated 501 diverse temperate maize inbred lines in five unique environments and predicted 16 compositional traits (e.g., carbohydrates, protein, and starch) based on the output of near-infrared (NIR) spectroscopy.
34197039	5	62	theme	different	1010:1018	arg1	types					1026:1030	different maize types	1010:1030	different maize types (e.g., dent, sweetcorn, and popcorn)	1010:1067	Correlations and trade-offs among traits in different maize types (e.g., dent, sweetcorn, and popcorn) were explored, and significant differences and meaningful correlations were detected.
34197039	8	63	theme	phenotypic	1547:1556	arg1	variation					1558:1566	phenotypic variation	1547:1566	phenotypic variation	1547:1566	This study provides valuable insights in the phenotypic variation and genetic control underlying compositional traits that can be used in breeding programs for improving maize grain quality.
34197039	4	64	theme	Phenotypic	811:820	arg1	analysis					822:829	Phenotypic analysis	811:829	Phenotypic analysis	811:829	Phenotypic analysis found substantial variation for compositional traits and the majority of variation was explained by genetic and environmental factors.
34197039	7	65	theme	same	1416:1419	arg1	markers					1421:1427	these same markers	1410:1427	these same markers	1410:1427	A genome-wide association study (GWAS) was conducted using these same markers and found 72 statistically significant SNPs for 11 compositional traits.
34197039	3	66	theme	grain	554:558	arg1	traits					574:579	grain compositional traits	554:579	grain compositional traits in maize	554:588	To better understand variation that exists for grain compositional traits in maize, we evaluated 501 diverse temperate maize inbred lines in five unique environments and predicted 16 compositional traits (e.g., carbohydrates, protein, and starch) based on the output of near-infrared (NIR) spectroscopy.
34197039	6	67	theme	polymorphism	1277:1288	arg1	markers					1296:1302	2,386,666 single nucleotide polymorphism (SNP) markers	1249:1302	2,386,666 single nucleotide polymorphism (SNP) markers generated from whole-genome resequencing data	1249:1348	In total, 22.9-71.0% of the phenotypic variation across these traits could be explained using 2,386,666 single nucleotide polymorphism (SNP) markers generated from whole-genome resequencing data.
34197039	8	68	theme	valuable	1522:1529	arg1	insights					1531:1538	valuable insights	1522:1538	valuable insights in the phenotypic variation and genetic control underlying compositional traits that can be used in breeding programs for improving maize grain quality	1522:1690	This study provides valuable insights in the phenotypic variation and genetic control underlying compositional traits that can be used in breeding programs for improving maize grain quality.
34197039	0	69	theme	diversity	61:69	arg1	panel					71:75	a maize diversity panel	53:75	a maize diversity panel	53:75	Genetic control of kernel compositional variation in a maize diversity panel.
34197039	8	70	used	used	1632:1635	arg2	traits					1613:1618	compositional traits	1599:1618	compositional traits that can be used in breeding programs for improving maize grain quality	1599:1690	This study provides valuable insights in the phenotypic variation and genetic control underlying compositional traits that can be used in breeding programs for improving maize grain quality.
34197039	2	71	theme	quality	304:310	arg1	traits					312:317	quality traits	304:317	quality traits	304:317	Some knowledge of the genetic factors that affect quality traits has been discovered through the study of classical maize mutants; however, much of the underlying genetic control of these traits and the interaction between these traits remains unknown.
34197039	8	72	theme	compositional	1599:1611	arg1	traits					1613:1618	compositional traits	1599:1618	compositional traits that can be used in breeding programs for improving maize grain quality	1599:1690	This study provides valuable insights in the phenotypic variation and genetic control underlying compositional traits that can be used in breeding programs for improving maize grain quality.
34197039	0	73	theme	maize	55:59	arg1	panel					71:75	a maize diversity panel	53:75	a maize diversity panel	53:75	Genetic control of kernel compositional variation in a maize diversity panel.
34197039	3	74	theme	maize	626:630	arg1	lines					639:643	501 diverse temperate maize inbred lines	604:643	501 diverse temperate maize inbred lines in five unique environments	604:671	To better understand variation that exists for grain compositional traits in maize, we evaluated 501 diverse temperate maize inbred lines in five unique environments and predicted 16 compositional traits (e.g., carbohydrates, protein, and starch) based on the output of near-infrared (NIR) spectroscopy.
34197039	1	75	theme	row	117:119	arg1	worldwide					132:140	a multi-purpose row crop grown worldwide	101:140	a multi-purpose row crop grown worldwide	101:140	Maize (Zea mays L.) is a multi-purpose row crop grown worldwide, which, over time, has often been bred for increased yield at the detriment of lower composition grain quality.
34197039	1	75	theme	row	117:119	arg1	Maize					78:82	Maize	78:82	Maize (Zea mays L.)	78:96	Maize (Zea mays L.) is a multi-purpose row crop grown worldwide, which, over time, has often been bred for increased yield at the detriment of lower composition grain quality.
34197039	8	76	theme	genetic	1572:1578	arg1	control					1580:1586	genetic control	1572:1586	genetic control	1572:1586	This study provides valuable insights in the phenotypic variation and genetic control underlying compositional traits that can be used in breeding programs for improving maize grain quality.
34197039	3	77	from	traits	574:579	arg1	maize					584:588	maize	584:588	maize	584:588	To better understand variation that exists for grain compositional traits in maize, we evaluated 501 diverse temperate maize inbred lines in five unique environments and predicted 16 compositional traits (e.g., carbohydrates, protein, and starch) based on the output of near-infrared (NIR) spectroscopy.
32921414	4	0	theme	epitope	645:651	arg1	region					653:658	the binding epitope region	633:658	the binding epitope region of LpMab-3 that includes the peptide (-67LVATSVNSV-T-GIRIEDLP84-) possessing a disialyl-core-1 O-glycan at Thr76	633:771	In this study, we synthesized the binding epitope region of LpMab-3 that includes the peptide (-67LVATSVNSV-T-GIRIEDLP84-) possessing a disialyl-core-1 O-glycan at Thr76, and we determined the crystal structure of the LpMab-3 Fab fragment that was bound to the synthesized glycopeptide at a 2.8 Å resolution.
32921414	4	1	theme	fragment	833:840	arg1	structure					804:812	the crystal structure	792:812	the crystal structure of the LpMab-3 Fab fragment that was bound to the synthesized glycopeptide at a 2.8 Å resolution	792:909	In this study, we synthesized the binding epitope region of LpMab-3 that includes the peptide (-67LVATSVNSV-T-GIRIEDLP84-) possessing a disialyl-core-1 O-glycan at Thr76, and we determined the crystal structure of the LpMab-3 Fab fragment that was bound to the synthesized glycopeptide at a 2.8 Å resolution.
32921414	4	2	theme	disialyl-core-1	739:753	arg1	O-glycan					755:762	a disialyl-core-1 O-glycan	737:762	a disialyl-core-1 O-glycan	737:762	In this study, we synthesized the binding epitope region of LpMab-3 that includes the peptide (-67LVATSVNSV-T-GIRIEDLP84-) possessing a disialyl-core-1 O-glycan at Thr76, and we determined the crystal structure of the LpMab-3 Fab fragment that was bound to the synthesized glycopeptide at a 2.8 Å resolution.
32921414	5	3	dep	regions	1031:1037	arg1	H3					1054:1055	H3	1054:1055	H3	1054:1055	The six amino acid residues and two sialic acid residues are directly associated with four complementarity-determining regions (CDRs; H1, H2, H3, and L3) and four CDRs (H2, H3, L1, and L3), respectively.
32921414	5	3	dep	regions	1031:1037	arg1	L3					1062:1063	L3	1062:1063	L3	1062:1063	The six amino acid residues and two sialic acid residues are directly associated with four complementarity-determining regions (CDRs; H1, H2, H3, and L3) and four CDRs (H2, H3, L1, and L3), respectively.
32921414	5	3	dep	regions	1031:1037	arg1	H1					1046:1047	H1	1046:1047	H1	1046:1047	The six amino acid residues and two sialic acid residues are directly associated with four complementarity-determining regions (CDRs; H1, H2, H3, and L3) and four CDRs (H2, H3, L1, and L3), respectively.
32921414	5	3	dep	regions	1031:1037	arg1	CDRs					1040:1043	CDRs	1040:1043	CDRs; H1, H2, H3, and L3	1040:1063	The six amino acid residues and two sialic acid residues are directly associated with four complementarity-determining regions (CDRs; H1, H2, H3, and L3) and four CDRs (H2, H3, L1, and L3), respectively.
32921414	0	4	theme	O-linked	73:80	arg1	glycopeptide					82:93	a disialylated O-linked glycopeptide	58:93	a disialylated O-linked glycopeptide	58:93	Crystal structure of an anti-podoplanin antibody bound to a disialylated O-linked glycopeptide.
32921414	4	5	theme	Fab	829:831	arg1	fragment					833:840	the LpMab-3 Fab fragment	817:840	the LpMab-3 Fab fragment that was bound to the synthesized glycopeptide at a 2.8 Å resolution	817:909	In this study, we synthesized the binding epitope region of LpMab-3 that includes the peptide (-67LVATSVNSV-T-GIRIEDLP84-) possessing a disialyl-core-1 O-glycan at Thr76, and we determined the crystal structure of the LpMab-3 Fab fragment that was bound to the synthesized glycopeptide at a 2.8 Å resolution.
32921414	5	6	theme	amino	920:924	arg1	residues					931:938	The six amino acid residues	912:938	The six amino acid residues	912:938	The six amino acid residues and two sialic acid residues are directly associated with four complementarity-determining regions (CDRs; H1, H2, H3, and L3) and four CDRs (H2, H3, L1, and L3), respectively.
32921414	0	7	gly	disialylated	60:71	arg1	glycopeptide					82:93	a disialylated O-linked glycopeptide	58:93	a disialylated O-linked glycopeptide	58:93	Crystal structure of an anti-podoplanin antibody bound to a disialylated O-linked glycopeptide.
32921414	2	8	theme	hPDPN	295:299	arg1	antibody					313:320	an anti-human PDPN (hPDPN) monoclonal antibody	275:320	an anti-human PDPN (hPDPN) monoclonal antibody (mAb)	275:326	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	2	8	theme	hPDPN	295:299	arg1	LpMab-3					335:341	clone LpMab-3	329:341	clone LpMab-3	329:341	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	2	8	theme	hPDPN	295:299	arg1	mAb					323:325	mAb	323:325	mAb	323:325	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	3	9	dep	peptides	581:588	arg1	the					577:579	the	577:579	the	577:579	However, it is unclear if the mAb binds directly to both the peptides and glycans.
32921414	2	10	theme	PDPN	289:292	arg1	antibody					313:320	an anti-human PDPN (hPDPN) monoclonal antibody	275:320	an anti-human PDPN (hPDPN) monoclonal antibody (mAb)	275:326	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	2	10	theme	PDPN	289:292	arg1	LpMab-3					335:341	clone LpMab-3	329:341	clone LpMab-3	329:341	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	2	10	theme	PDPN	289:292	arg1	mAb					323:325	mAb	323:325	mAb	323:325	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	2	11	theme	NeuAcα2-3Galβl-3	435:450	arg1	structure					479:487	the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure	406:487	the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure	406:487	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	5	12	dep	CDRs	1075:1078	arg1	L3					1097:1098	L3	1097:1098	L3	1097:1098	The six amino acid residues and two sialic acid residues are directly associated with four complementarity-determining regions (CDRs; H1, H2, H3, and L3) and four CDRs (H2, H3, L1, and L3), respectively.
32921414	5	12	dep	CDRs	1075:1078	arg1	H3					1085:1086	H3	1085:1086	H3	1085:1086	The six amino acid residues and two sialic acid residues are directly associated with four complementarity-determining regions (CDRs; H1, H2, H3, and L3) and four CDRs (H2, H3, L1, and L3), respectively.
32921414	5	12	dep	CDRs	1075:1078	arg1	CDRs					1075:1078	four CDRs	1070:1078	four CDRs (H2, H3, L1, and L3)	1070:1099	The six amino acid residues and two sialic acid residues are directly associated with four complementarity-determining regions (CDRs; H1, H2, H3, and L3) and four CDRs (H2, H3, L1, and L3), respectively.
32921414	5	12	dep	CDRs	1075:1078	arg1	L1					1089:1090	L1	1089:1090	L1	1089:1090	The six amino acid residues and two sialic acid residues are directly associated with four complementarity-determining regions (CDRs; H1, H2, H3, and L3) and four CDRs (H2, H3, L1, and L3), respectively.
32921414	4	13	theme	LpMab-3	821:827	arg1	fragment					833:840	the LpMab-3 Fab fragment	817:840	the LpMab-3 Fab fragment that was bound to the synthesized glycopeptide at a 2.8 Å resolution	817:909	In this study, we synthesized the binding epitope region of LpMab-3 that includes the peptide (-67LVATSVNSV-T-GIRIEDLP84-) possessing a disialyl-core-1 O-glycan at Thr76, and we determined the crystal structure of the LpMab-3 Fab fragment that was bound to the synthesized glycopeptide at a 2.8 Å resolution.
32921414	2	14	theme	Thr76	496:500	arg1	residue					502:508	the Thr76 residue	492:508	the Thr76 residue in hPDPN	492:517	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	6	15	theme	spacious	1194:1201	arg1	epitopes					1203:1210	spacious epitopes	1194:1210	spacious epitopes such as glycoconjugates	1194:1234	These results suggest that IgG is advantageous for generating binders against spacious epitopes such as glycoconjugates.
32921414	6	15	theme	spacious	1194:1201	arg1	glycoconjugates					1220:1234	glycoconjugates	1220:1234	glycoconjugates	1220:1234	These results suggest that IgG is advantageous for generating binders against spacious epitopes such as glycoconjugates.
32921414	0	16	theme	Crystal	0:6	arg1	structure					8:16	Crystal structure	0:16	Crystal structure of an anti-podoplanin antibody	0:47	Crystal structure of an anti-podoplanin antibody bound to a disialylated O-linked glycopeptide.
32921414	1	17	theme	specific	176:183	arg1	glycoprotein					141:152	a highly O-glycosylated glycoprotein	117:152	a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions	117:248	Podoplanin (PDPN) is a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions.
32921414	1	17	theme	specific	176:183	arg1	marker					207:212	a specific lymphatic endothelial marker	174:212	a specific lymphatic endothelial marker under pathophysiological conditions	174:248	Podoplanin (PDPN) is a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions.
32921414	2	18	theme	disialy-core-l	419:432	arg1	structure					479:487	the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure	406:487	the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure	406:487	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	1	19	theme	lymphatic	185:193	arg1	glycoprotein					141:152	a highly O-glycosylated glycoprotein	117:152	a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions	117:248	Podoplanin (PDPN) is a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions.
32921414	1	19	theme	lymphatic	185:193	arg1	marker					207:212	a specific lymphatic endothelial marker	174:212	a specific lymphatic endothelial marker under pathophysiological conditions	174:248	Podoplanin (PDPN) is a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions.
32921414	2	20	theme	attached	410:417	arg1	structure					479:487	the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure	406:487	the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure	406:487	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	2	21	theme	monoclonal	302:311	arg1	antibody					313:320	an anti-human PDPN (hPDPN) monoclonal antibody	275:320	an anti-human PDPN (hPDPN) monoclonal antibody (mAb)	275:326	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	2	21	theme	monoclonal	302:311	arg1	LpMab-3					335:341	clone LpMab-3	329:341	clone LpMab-3	329:341	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	2	21	theme	monoclonal	302:311	arg1	mAb					323:325	mAb	323:325	mAb	323:325	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	1	22	theme	endothelial	195:205	arg1	glycoprotein					141:152	a highly O-glycosylated glycoprotein	117:152	a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions	117:248	Podoplanin (PDPN) is a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions.
32921414	1	22	theme	endothelial	195:205	arg1	marker					207:212	a specific lymphatic endothelial marker	174:212	a specific lymphatic endothelial marker under pathophysiological conditions	174:248	Podoplanin (PDPN) is a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions.
32921414	0	23	theme	antibody	40:47	arg1	structure					8:16	Crystal structure	0:16	Crystal structure of an anti-podoplanin antibody	0:47	Crystal structure of an anti-podoplanin antibody bound to a disialylated O-linked glycopeptide.
32921414	4	24	theme	LpMab-3	663:669	arg1	region					653:658	the binding epitope region	633:658	the binding epitope region of LpMab-3 that includes the peptide (-67LVATSVNSV-T-GIRIEDLP84-) possessing a disialyl-core-1 O-glycan at Thr76	633:771	In this study, we synthesized the binding epitope region of LpMab-3 that includes the peptide (-67LVATSVNSV-T-GIRIEDLP84-) possessing a disialyl-core-1 O-glycan at Thr76, and we determined the crystal structure of the LpMab-3 Fab fragment that was bound to the synthesized glycopeptide at a 2.8 Å resolution.
32921414	4	25	theme	2.8 Å	894:898	arg1	resolution					900:909	a 2.8 Å resolution	892:909	a 2.8 Å resolution	892:909	In this study, we synthesized the binding epitope region of LpMab-3 that includes the peptide (-67LVATSVNSV-T-GIRIEDLP84-) possessing a disialyl-core-1 O-glycan at Thr76, and we determined the crystal structure of the LpMab-3 Fab fragment that was bound to the synthesized glycopeptide at a 2.8 Å resolution.
32921414	0	26	theme	anti-podoplanin	24:38	arg1	antibody					40:47	an anti-podoplanin antibody	21:47	an anti-podoplanin antibody	21:47	Crystal structure of an anti-podoplanin antibody bound to a disialylated O-linked glycopeptide.
32921414	2	27	theme	GalNAcαl-O-Thr	463:476	arg1	structure					479:487	the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure	406:487	the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure	406:487	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	1	28	gly	glycoprotein	141:152	arg1	glycoprotein					141:152	a highly O-glycosylated glycoprotein	117:152	a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions	117:248	Podoplanin (PDPN) is a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions.
32921414	1	28	gly	glycoprotein	141:152	arg1	marker					207:212	a specific lymphatic endothelial marker	174:212	a specific lymphatic endothelial marker under pathophysiological conditions	174:248	Podoplanin (PDPN) is a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions.
32921414	1	28	gly	glycoprotein	141:152	arg1	Podoplanin					96:105	Podoplanin	96:105	Podoplanin (PDPN)	96:112	Podoplanin (PDPN) is a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions.
32921414	4	29	theme	crystal	796:802	arg1	structure					804:812	the crystal structure	792:812	the crystal structure of the LpMab-3 Fab fragment that was bound to the synthesized glycopeptide at a 2.8 Å resolution	792:909	In this study, we synthesized the binding epitope region of LpMab-3 that includes the peptide (-67LVATSVNSV-T-GIRIEDLP84-) possessing a disialyl-core-1 O-glycan at Thr76, and we determined the crystal structure of the LpMab-3 Fab fragment that was bound to the synthesized glycopeptide at a 2.8 Å resolution.
32921414	0	30	link	O-linked	73:80	arg1	glycopeptide					82:93	a disialylated O-linked glycopeptide	58:93	a disialylated O-linked glycopeptide	58:93	Crystal structure of an anti-podoplanin antibody bound to a disialylated O-linked glycopeptide.
32921414	4	31	gly	glycopeptide	876:887	arg2	glycopeptide					876:887	the synthesized glycopeptide	860:887	the synthesized glycopeptide	860:887	In this study, we synthesized the binding epitope region of LpMab-3 that includes the peptide (-67LVATSVNSV-T-GIRIEDLP84-) possessing a disialyl-core-1 O-glycan at Thr76, and we determined the crystal structure of the LpMab-3 Fab fragment that was bound to the synthesized glycopeptide at a 2.8 Å resolution.
32921414	2	32	theme	[NeuAcα2-6	452:461	arg1	structure					479:487	the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure	406:487	the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure	406:487	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	4	33	contain	possessing	726:735	arg2	O-glycan					755:762	a disialyl-core-1 O-glycan	737:762	a disialyl-core-1 O-glycan	737:762	In this study, we synthesized the binding epitope region of LpMab-3 that includes the peptide (-67LVATSVNSV-T-GIRIEDLP84-) possessing a disialyl-core-1 O-glycan at Thr76, and we determined the crystal structure of the LpMab-3 Fab fragment that was bound to the synthesized glycopeptide at a 2.8 Å resolution.
32921414	4	33	contain	possessing	726:735	arg1	-67LVATSVNSV-T-GIRIEDLP84-					698:723	-67LVATSVNSV-T-GIRIEDLP84-	698:723	-67LVATSVNSV-T-GIRIEDLP84-	698:723	In this study, we synthesized the binding epitope region of LpMab-3 that includes the peptide (-67LVATSVNSV-T-GIRIEDLP84-) possessing a disialyl-core-1 O-glycan at Thr76, and we determined the crystal structure of the LpMab-3 Fab fragment that was bound to the synthesized glycopeptide at a 2.8 Å resolution.
32921414	4	33	contain	possessing	726:735	arg1	peptide					689:695	the peptide	685:695	the peptide (-67LVATSVNSV-T-GIRIEDLP84-) possessing a disialyl-core-1 O-glycan at Thr76	685:771	In this study, we synthesized the binding epitope region of LpMab-3 that includes the peptide (-67LVATSVNSV-T-GIRIEDLP84-) possessing a disialyl-core-1 O-glycan at Thr76, and we determined the crystal structure of the LpMab-3 Fab fragment that was bound to the synthesized glycopeptide at a 2.8 Å resolution.
32921414	1	34	gly	O-glycosylated	126:139	arg1	glycoprotein					141:152	a highly O-glycosylated glycoprotein	117:152	a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions	117:248	Podoplanin (PDPN) is a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions.
32921414	1	34	gly	O-glycosylated	126:139	arg1	marker					207:212	a specific lymphatic endothelial marker	174:212	a specific lymphatic endothelial marker under pathophysiological conditions	174:248	Podoplanin (PDPN) is a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions.
32921414	1	34	gly	O-glycosylated	126:139	arg1	Podoplanin					96:105	Podoplanin	96:105	Podoplanin (PDPN)	96:112	Podoplanin (PDPN) is a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions.
32921414	5	35	theme	complementarity-determining	1003:1029	arg1	regions					1031:1037	four complementarity-determining regions	998:1037	four complementarity-determining regions (CDRs; H1, H2, H3, and L3)	998:1064	The six amino acid residues and two sialic acid residues are directly associated with four complementarity-determining regions (CDRs; H1, H2, H3, and L3) and four CDRs (H2, H3, L1, and L3), respectively.
32921414	2	36	theme	clone	329:333	arg1	antibody					313:320	an anti-human PDPN (hPDPN) monoclonal antibody	275:320	an anti-human PDPN (hPDPN) monoclonal antibody (mAb)	275:326	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	2	36	theme	clone	329:333	arg1	LpMab-3					335:341	clone LpMab-3	329:341	clone LpMab-3	329:341	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	4	37	theme	synthesized	864:874	arg1	glycopeptide					876:887	the synthesized glycopeptide	860:887	the synthesized glycopeptide	860:887	In this study, we synthesized the binding epitope region of LpMab-3 that includes the peptide (-67LVATSVNSV-T-GIRIEDLP84-) possessing a disialyl-core-1 O-glycan at Thr76, and we determined the crystal structure of the LpMab-3 Fab fragment that was bound to the synthesized glycopeptide at a 2.8 Å resolution.
32921414	2	38	from	residue	502:508	arg1	hPDPN					513:517	hPDPN	513:517	hPDPN	513:517	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	2	39	theme	anti-human	278:287	arg1	antibody					313:320	an anti-human PDPN (hPDPN) monoclonal antibody	275:320	an anti-human PDPN (hPDPN) monoclonal antibody (mAb)	275:326	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	2	39	theme	anti-human	278:287	arg1	LpMab-3					335:341	clone LpMab-3	329:341	clone LpMab-3	329:341	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	2	39	theme	anti-human	278:287	arg1	mAb					323:325	mAb	323:325	mAb	323:325	We previously developed an anti-human PDPN (hPDPN) monoclonal antibody (mAb), clone LpMab-3, which recognizes the epitope, including both the peptides and the attached disialy-core-l (NeuAcα2-3Galβl-3 [NeuAcα2-6]GalNAcαl-O-Thr) structure at the Thr76 residue in hPDPN.
32921414	1	40	theme	pathophysiological	220:237	arg1	conditions					239:248	pathophysiological conditions	220:248	pathophysiological conditions	220:248	Podoplanin (PDPN) is a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions.
32921414	0	41	gly	glycopeptide	82:93	arg2	glycopeptide					82:93	a disialylated O-linked glycopeptide	58:93	a disialylated O-linked glycopeptide	58:93	Crystal structure of an anti-podoplanin antibody bound to a disialylated O-linked glycopeptide.
32921414	5	42	theme	sialic	948:953	arg1	residues					960:967	two sialic acid residues	944:967	two sialic acid residues	944:967	The six amino acid residues and two sialic acid residues are directly associated with four complementarity-determining regions (CDRs; H1, H2, H3, and L3) and four CDRs (H2, H3, L1, and L3), respectively.
32921414	0	43	theme	disialylated	60:71	arg1	glycopeptide					82:93	a disialylated O-linked glycopeptide	58:93	a disialylated O-linked glycopeptide	58:93	Crystal structure of an anti-podoplanin antibody bound to a disialylated O-linked glycopeptide.
32921414	5	44	theme	acid	955:958	arg1	residues					960:967	two sialic acid residues	944:967	two sialic acid residues	944:967	The six amino acid residues and two sialic acid residues are directly associated with four complementarity-determining regions (CDRs; H1, H2, H3, and L3) and four CDRs (H2, H3, L1, and L3), respectively.
32921414	1	45	theme	O-glycosylated	126:139	arg1	glycoprotein					141:152	a highly O-glycosylated glycoprotein	117:152	a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions	117:248	Podoplanin (PDPN) is a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions.
32921414	1	45	theme	O-glycosylated	126:139	arg1	marker					207:212	a specific lymphatic endothelial marker	174:212	a specific lymphatic endothelial marker under pathophysiological conditions	174:248	Podoplanin (PDPN) is a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions.
32921414	1	45	theme	O-glycosylated	126:139	arg1	Podoplanin					96:105	Podoplanin	96:105	Podoplanin (PDPN)	96:112	Podoplanin (PDPN) is a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions.
32921414	5	46	dep	CDRs	1040:1043	arg1	H3					1054:1055	H3	1054:1055	H3	1054:1055	The six amino acid residues and two sialic acid residues are directly associated with four complementarity-determining regions (CDRs; H1, H2, H3, and L3) and four CDRs (H2, H3, L1, and L3), respectively.
32921414	5	46	dep	CDRs	1040:1043	arg1	L3					1062:1063	L3	1062:1063	L3	1062:1063	The six amino acid residues and two sialic acid residues are directly associated with four complementarity-determining regions (CDRs; H1, H2, H3, and L3) and four CDRs (H2, H3, L1, and L3), respectively.
32921414	5	46	dep	CDRs	1040:1043	arg1	H1					1046:1047	H1	1046:1047	H1	1046:1047	The six amino acid residues and two sialic acid residues are directly associated with four complementarity-determining regions (CDRs; H1, H2, H3, and L3) and four CDRs (H2, H3, L1, and L3), respectively.
32921414	5	46	dep	CDRs	1040:1043	arg1	CDRs					1040:1043	CDRs	1040:1043	CDRs; H1, H2, H3, and L3	1040:1063	The six amino acid residues and two sialic acid residues are directly associated with four complementarity-determining regions (CDRs; H1, H2, H3, and L3) and four CDRs (H2, H3, L1, and L3), respectively.
32921414	5	47	theme	acid	926:929	arg1	residues					931:938	The six amino acid residues	912:938	The six amino acid residues	912:938	The six amino acid residues and two sialic acid residues are directly associated with four complementarity-determining regions (CDRs; H1, H2, H3, and L3) and four CDRs (H2, H3, L1, and L3), respectively.
32921414	1	48	used	utilized	162:169	arg2	glycoprotein					141:152	a highly O-glycosylated glycoprotein	117:152	a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions	117:248	Podoplanin (PDPN) is a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions.
32921414	1	48	used	utilized	162:169	arg2	marker					207:212	a specific lymphatic endothelial marker	174:212	a specific lymphatic endothelial marker under pathophysiological conditions	174:248	Podoplanin (PDPN) is a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions.
32921414	1	48	used	utilized	162:169	arg2	Podoplanin					96:105	Podoplanin	96:105	Podoplanin (PDPN)	96:112	Podoplanin (PDPN) is a highly O-glycosylated glycoprotein that is utilized as a specific lymphatic endothelial marker under pathophysiological conditions.
32921414	4	49	theme	binding	637:643	arg1	region					653:658	the binding epitope region	633:658	the binding epitope region of LpMab-3 that includes the peptide (-67LVATSVNSV-T-GIRIEDLP84-) possessing a disialyl-core-1 O-glycan at Thr76	633:771	In this study, we synthesized the binding epitope region of LpMab-3 that includes the peptide (-67LVATSVNSV-T-GIRIEDLP84-) possessing a disialyl-core-1 O-glycan at Thr76, and we determined the crystal structure of the LpMab-3 Fab fragment that was bound to the synthesized glycopeptide at a 2.8 Å resolution.
32992132	7	0	theme	significant	1308:1318	arg1	correlation					1320:1330	a significant correlation	1306:1330	a significant correlation with hemicellulose activity	1306:1358	In terms of cell wall-related enzymes, 'Yushu No 10' hardness gumminess and chewiness had a significant correlation with hemicellulose activity, and 'Mianfen No 1' had insignificant correlation with four cell wall-related enzymes.
32992132	9	1	theme	structure	1706:1714	arg1	changes					1716:1722	the qualitative structure changes	1690:1722	the qualitative structure changes	1690:1722	The expressions of IbEXP1, IbEXP2 and IbEXPL1 were significantly correlated with the changes in cell wall component content, and were related to the qualitative structure changes.
32992132	10	2	theme	research	1729:1736	arg1	conclusion					1738:1747	The research conclusion	1725:1747	The research conclusion	1725:1747	The research conclusion shows that the texture changes during the growth of sweetpotato are related to cell wall composition, cell wall-related enzyme activity changes, and the expression of expansin genes.
32992132	6	3	theme	No	1153:1154	arg1	content					1135:1141	the cellulose content	1121:1141	the cellulose content of 'Yushu No 10'	1121:1158	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	6	3	theme	No	1153:1154	arg1	higher					1180:1185	higher	1180:1185	higher	1180:1185	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	8	4	theme	root	1523:1526	arg1	expansin					1535:1542	root tubers expansin	1523:1542	root tubers expansin	1523:1542	Expansin genes were also expressed differently during the various stages of root tubers expansin.
32992132	1	5	theme	sweetpotato	279:289	arg1	quality					268:274	post-production processing quality	241:274	post-production processing quality	241:274	Sweetpotato has special texture characteristics, which directly affect the eating quality and post-production processing quality of sweetpotato.
32992132	1	5	theme	sweetpotato	279:289	arg1	quality					229:235	eating quality	222:235	eating quality	222:235	Sweetpotato has special texture characteristics, which directly affect the eating quality and post-production processing quality of sweetpotato.
32992132	7	6	theme	No	1262:1263	arg1	gumminess					1278:1286	'Yushu No 10' hardness gumminess	1255:1286	'Yushu No 10' hardness gumminess	1255:1286	In terms of cell wall-related enzymes, 'Yushu No 10' hardness gumminess and chewiness had a significant correlation with hemicellulose activity, and 'Mianfen No 1' had insignificant correlation with four cell wall-related enzymes.
32992132	4	7	theme	wall	588:591	arg1	content					605:611	the cell wall composition content	579:611	the cell wall composition content	579:611	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	4	7	theme	wall	588:591	arg1	parameters					567:576	the texture parameters	555:576	the texture parameters	555:576	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	2	8	theme	texture	311:317	arg1	mechanism					326:334	the texture change mechanism	307:334	the texture change mechanism of sweetpotato	307:349	To investigate the texture change mechanism of sweetpotato during the growth process, this study selected two varieties with significant differences in texture from 35 varieties.
32992132	7	9	with	correlation	1320:1330	arg1	activity					1351:1358	hemicellulose activity	1337:1358	hemicellulose activity	1337:1358	In terms of cell wall-related enzymes, 'Yushu No 10' hardness gumminess and chewiness had a significant correlation with hemicellulose activity, and 'Mianfen No 1' had insignificant correlation with four cell wall-related enzymes.
32992132	10	10	theme	expansin	1916:1923	arg1	genes					1925:1929	expansin genes	1916:1929	expansin genes	1916:1929	The research conclusion shows that the texture changes during the growth of sweetpotato are related to cell wall composition, cell wall-related enzyme activity changes, and the expression of expansin genes.
32992132	1	11	contain	has	159:161	arg2	characteristics					179:193	special texture characteristics	163:193	special texture characteristics	163:193	Sweetpotato has special texture characteristics, which directly affect the eating quality and post-production processing quality of sweetpotato.
32992132	1	11	contain	has	159:161	arg1	Sweetpotato					147:157	Sweetpotato	147:157	Sweetpotato	147:157	Sweetpotato has special texture characteristics, which directly affect the eating quality and post-production processing quality of sweetpotato.
32992132	7	12	theme	hardness	1269:1276	arg1	gumminess					1278:1286	'Yushu No 10' hardness gumminess	1255:1286	'Yushu No 10' hardness gumminess	1255:1286	In terms of cell wall-related enzymes, 'Yushu No 10' hardness gumminess and chewiness had a significant correlation with hemicellulose activity, and 'Mianfen No 1' had insignificant correlation with four cell wall-related enzymes.
32992132	11	13	theme	theoretical	1952:1962	arg1	guidance					1964:1971	theoretical guidance	1952:1971	theoretical guidance for in-depth study of texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots	1952:2106	This study provides theoretical guidance for in-depth study of texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots.
32992132	7	14	theme	enzymes	1246:1252	arg1	terms					1219:1223	terms	1219:1223	terms of cell wall-related enzymes	1219:1252	In terms of cell wall-related enzymes, 'Yushu No 10' hardness gumminess and chewiness had a significant correlation with hemicellulose activity, and 'Mianfen No 1' had insignificant correlation with four cell wall-related enzymes.
32992132	4	15	theme	expansin	672:679	arg1	genes					681:685	expansin genes	672:685	expansin genes	672:685	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	2	16	theme	sweetpotato	339:349	arg1	mechanism					326:334	the texture change mechanism	307:334	the texture change mechanism of sweetpotato	307:349	To investigate the texture change mechanism of sweetpotato during the growth process, this study selected two varieties with significant differences in texture from 35 varieties.
32992132	6	17	theme	wall	934:937	arg1	composition					939:949	cell wall composition	929:949	cell wall composition	929:949	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	6	18	theme	insoluble	1052:1060	arg1	lower					1081:1085	lower	1081:1085	lower	1081:1085	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	6	18	theme	insoluble	1052:1060	arg1	content					1069:1075	the insoluble pectin content	1048:1075	the insoluble pectin content	1048:1075	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	4	19	theme	enzyme	632:637	arg1	activities					639:648	cell wall-related enzyme activities	614:648	cell wall-related enzyme activities	614:648	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	4	19	theme	enzyme	632:637	arg1	parameters					567:576	the texture parameters	555:576	the texture parameters	555:576	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	0	20	theme	storage	126:132	arg1	growth					139:144	sweetpotato storage root growth	114:144	sweetpotato storage root growth	114:144	Changes in cell wall components and polysaccharide-degrading enzymes in relation to differences in texture during sweetpotato storage root growth.
32992132	7	21	theme	wall-related	1425:1436	arg1	enzymes					1438:1444	four cell wall-related enzymes	1415:1444	four cell wall-related enzymes	1415:1444	In terms of cell wall-related enzymes, 'Yushu No 10' hardness gumminess and chewiness had a significant correlation with hemicellulose activity, and 'Mianfen No 1' had insignificant correlation with four cell wall-related enzymes.
32992132	9	22	theme	IbEXP1	1564:1569	arg1	related					1679:1685	related	1679:1685	related	1679:1685	The expressions of IbEXP1, IbEXP2 and IbEXPL1 were significantly correlated with the changes in cell wall component content, and were related to the qualitative structure changes.
32992132	9	22	theme	IbEXP1	1564:1569	arg1	expressions					1549:1559	The expressions	1545:1559	The expressions of IbEXP1, IbEXP2 and IbEXPL1	1545:1589	The expressions of IbEXP1, IbEXP2 and IbEXPL1 were significantly correlated with the changes in cell wall component content, and were related to the qualitative structure changes.
32992132	5	23	contain	have	868:871	arg2	properties					905:914	significantly different texture properties	873:914	significantly different texture properties	873:914	The results show that the hardness, adhesiveness and chewiness parameters of 'Yushu No 10' were significantly lower than those of 'Mianfen No 1', they have significantly different texture properties.
32992132	5	23	contain	have	868:871	arg1	they					863:866	they	863:866	they	863:866	The results show that the hardness, adhesiveness and chewiness parameters of 'Yushu No 10' were significantly lower than those of 'Mianfen No 1', they have significantly different texture properties.
32992132	5	24	theme	texture	897:903	arg1	properties					905:914	significantly different texture properties	873:914	significantly different texture properties	873:914	The results show that the hardness, adhesiveness and chewiness parameters of 'Yushu No 10' were significantly lower than those of 'Mianfen No 1', they have significantly different texture properties.
32992132	9	25	theme	IbEXP2	1572:1577	arg1	related					1679:1685	related	1679:1685	related	1679:1685	The expressions of IbEXP1, IbEXP2 and IbEXPL1 were significantly correlated with the changes in cell wall component content, and were related to the qualitative structure changes.
32992132	9	25	theme	IbEXP2	1572:1577	arg1	expressions					1549:1559	The expressions	1545:1559	The expressions of IbEXP1, IbEXP2 and IbEXPL1	1545:1589	The expressions of IbEXP1, IbEXP2 and IbEXPL1 were significantly correlated with the changes in cell wall component content, and were related to the qualitative structure changes.
32992132	10	26	theme	wall	1833:1836	arg1	composition					1838:1848	cell wall composition	1828:1848	cell wall composition	1828:1848	The research conclusion shows that the texture changes during the growth of sweetpotato are related to cell wall composition, cell wall-related enzyme activity changes, and the expression of expansin genes.
32992132	5	27	theme	adhesiveness	753:764	arg1	parameters					780:789	the hardness, adhesiveness and chewiness parameters	739:789	the hardness, adhesiveness and chewiness parameters of 'Yushu No 10'	739:806	The results show that the hardness, adhesiveness and chewiness parameters of 'Yushu No 10' were significantly lower than those of 'Mianfen No 1', they have significantly different texture properties.
32992132	5	27	theme	adhesiveness	753:764	arg1	lower					827:831	lower	827:831	lower	827:831	The results show that the hardness, adhesiveness and chewiness parameters of 'Yushu No 10' were significantly lower than those of 'Mianfen No 1', they have significantly different texture properties.
32992132	9	28	theme	IbEXPL1	1583:1589	arg1	related					1679:1685	related	1679:1685	related	1679:1685	The expressions of IbEXP1, IbEXP2 and IbEXPL1 were significantly correlated with the changes in cell wall component content, and were related to the qualitative structure changes.
32992132	9	28	theme	IbEXPL1	1583:1589	arg1	expressions					1549:1559	The expressions	1545:1559	The expressions of IbEXP1, IbEXP2 and IbEXPL1	1545:1589	The expressions of IbEXP1, IbEXP2 and IbEXPL1 were significantly correlated with the changes in cell wall component content, and were related to the qualitative structure changes.
32992132	2	29	from	varieties	460:468	arg1	texture					444:450	texture	444:450	texture from 35 varieties	444:468	To investigate the texture change mechanism of sweetpotato during the growth process, this study selected two varieties with significant differences in texture from 35 varieties.
32992132	2	29	from	varieties	460:468	arg1	differences					429:439	significant differences	417:439	significant differences in texture from 35 varieties	417:468	To investigate the texture change mechanism of sweetpotato during the growth process, this study selected two varieties with significant differences in texture from 35 varieties.
32992132	4	30	theme	storage	702:708	arg1	roots					710:714	sweetpotato storage roots	690:714	sweetpotato storage roots	690:714	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	10	31	theme	activity	1876:1883	arg1	changes					1885:1891	cell wall-related enzyme activity changes	1851:1891	cell wall-related enzyme activity changes	1851:1891	The research conclusion shows that the texture changes during the growth of sweetpotato are related to cell wall composition, cell wall-related enzyme activity changes, and the expression of expansin genes.
32992132	5	32	theme	chewiness	770:778	arg1	parameters					780:789	the hardness, adhesiveness and chewiness parameters	739:789	the hardness, adhesiveness and chewiness parameters of 'Yushu No 10'	739:806	The results show that the hardness, adhesiveness and chewiness parameters of 'Yushu No 10' were significantly lower than those of 'Mianfen No 1', they have significantly different texture properties.
32992132	5	32	theme	chewiness	770:778	arg1	lower					827:831	lower	827:831	lower	827:831	The results show that the hardness, adhesiveness and chewiness parameters of 'Yushu No 10' were significantly lower than those of 'Mianfen No 1', they have significantly different texture properties.
32992132	1	33	theme	texture	171:177	arg1	characteristics					179:193	special texture characteristics	163:193	special texture characteristics	163:193	Sweetpotato has special texture characteristics, which directly affect the eating quality and post-production processing quality of sweetpotato.
32992132	6	34	theme	soluble	956:962	arg1	more					1000:1003	more	1000:1003	more	1000:1003	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	6	34	theme	soluble	956:962	arg1	content					971:977	the soluble pectin content	952:977	the soluble pectin content of 'Yushu No 10'	952:994	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	0	35	from	Changes	0:6	arg1	relation					72:79	relation	72:79	relation to differences in texture during sweetpotato storage root growth	72:144	Changes in cell wall components and polysaccharide-degrading enzymes in relation to differences in texture during sweetpotato storage root growth.
32992132	0	35	from	Changes	0:6	arg1	components					21:30	cell wall components	11:30	cell wall components	11:30	Changes in cell wall components and polysaccharide-degrading enzymes in relation to differences in texture during sweetpotato storage root growth.
32992132	0	35	from	Changes	0:6	arg1	enzymes					61:67	polysaccharide-degrading enzymes	36:67	polysaccharide-degrading enzymes	36:67	Changes in cell wall components and polysaccharide-degrading enzymes in relation to differences in texture during sweetpotato storage root growth.
32992132	10	36	theme	wall-related	1856:1867	arg1	changes					1885:1891	cell wall-related enzyme activity changes	1851:1891	cell wall-related enzyme activity changes	1851:1891	The research conclusion shows that the texture changes during the growth of sweetpotato are related to cell wall composition, cell wall-related enzyme activity changes, and the expression of expansin genes.
32992132	8	37	theme	expansin	1535:1542	arg1	stages					1513:1518	the various stages	1501:1518	the various stages of root tubers expansin	1501:1542	Expansin genes were also expressed differently during the various stages of root tubers expansin.
32992132	5	38	theme	hardness	743:750	arg1	parameters					780:789	the hardness, adhesiveness and chewiness parameters	739:789	the hardness, adhesiveness and chewiness parameters of 'Yushu No 10'	739:806	The results show that the hardness, adhesiveness and chewiness parameters of 'Yushu No 10' were significantly lower than those of 'Mianfen No 1', they have significantly different texture properties.
32992132	5	38	theme	hardness	743:750	arg1	lower					827:831	lower	827:831	lower	827:831	The results show that the hardness, adhesiveness and chewiness parameters of 'Yushu No 10' were significantly lower than those of 'Mianfen No 1', they have significantly different texture properties.
32992132	11	39	theme	texture	1995:2001	arg1	changes					2003:2009	texture changes	1995:2009	texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots	1995:2106	This study provides theoretical guidance for in-depth study of texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots.
32992132	11	40	theme	utilization	2055:2065	arg1	changes					2003:2009	texture changes	1995:2009	texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots	1995:2106	This study provides theoretical guidance for in-depth study of texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots.
32992132	9	41	theme	cell	1641:1644	arg1	content					1661:1667	cell wall component content	1641:1667	cell wall component content	1641:1667	The expressions of IbEXP1, IbEXP2 and IbEXPL1 were significantly correlated with the changes in cell wall component content, and were related to the qualitative structure changes.
32992132	0	42	theme	cell	11:14	arg1	components					21:30	cell wall components	11:30	cell wall components	11:30	Changes in cell wall components and polysaccharide-degrading enzymes in relation to differences in texture during sweetpotato storage root growth.
32992132	9	43	theme	component	1651:1659	arg1	content					1661:1667	cell wall component content	1641:1667	cell wall component content	1641:1667	The expressions of IbEXP1, IbEXP2 and IbEXPL1 were significantly correlated with the changes in cell wall component content, and were related to the qualitative structure changes.
32992132	4	44	theme	texture	559:565	arg1	activities					639:648	cell wall-related enzyme activities	614:648	cell wall-related enzyme activities	614:648	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	4	44	theme	texture	559:565	arg1	expression					658:667	the expression	654:667	the expression of expansin genes of sweetpotato storage roots	654:714	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	4	44	theme	texture	559:565	arg1	content					605:611	the cell wall composition content	579:611	the cell wall composition content	579:611	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	4	44	theme	texture	559:565	arg1	parameters					567:576	the texture parameters	555:576	the texture parameters	555:576	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	11	45	dep	utilization	2055:2065	arg1	improvement					2079:2089	improvement	2079:2089	improvement	2079:2089	This study provides theoretical guidance for in-depth study of texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots.
32992132	6	46	theme	No	989:990	arg1	more					1000:1003	more	1000:1003	more	1000:1003	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	6	46	theme	No	989:990	arg1	content					971:977	the soluble pectin content	952:977	the soluble pectin content of 'Yushu No 10'	952:994	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	5	47	theme	Yushu	795:799	arg1	No					801:802	Yushu No 10	795:805	'Yushu No 10'	794:806	The results show that the hardness, adhesiveness and chewiness parameters of 'Yushu No 10' were significantly lower than those of 'Mianfen No 1', they have significantly different texture properties.
32992132	7	48	theme	hemicellulose	1337:1349	arg1	activity					1351:1358	hemicellulose activity	1337:1358	hemicellulose activity	1337:1358	In terms of cell wall-related enzymes, 'Yushu No 10' hardness gumminess and chewiness had a significant correlation with hemicellulose activity, and 'Mianfen No 1' had insignificant correlation with four cell wall-related enzymes.
32992132	2	49	from	differences	429:439	arg1	texture					444:450	texture	444:450	texture from 35 varieties	444:468	To investigate the texture change mechanism of sweetpotato during the growth process, this study selected two varieties with significant differences in texture from 35 varieties.
32992132	2	49	from	differences	429:439	arg1	varieties					460:468	35 varieties	457:468	35 varieties	457:468	To investigate the texture change mechanism of sweetpotato during the growth process, this study selected two varieties with significant differences in texture from 35 varieties.
32992132	2	50	theme	growth	362:367	arg1	process					369:375	the growth process	358:375	the growth process	358:375	To investigate the texture change mechanism of sweetpotato during the growth process, this study selected two varieties with significant differences in texture from 35 varieties.
32992132	4	51	theme	composition	593:603	arg1	content					605:611	the cell wall composition content	579:611	the cell wall composition content	579:611	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	4	51	theme	composition	593:603	arg1	parameters					567:576	the texture parameters	555:576	the texture parameters	555:576	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	1	52	theme	processing	257:266	arg1	quality					268:274	post-production processing quality	241:274	post-production processing quality	241:274	Sweetpotato has special texture characteristics, which directly affect the eating quality and post-production processing quality of sweetpotato.
32992132	6	53	theme	Mianfen	1025:1031	arg1	No					1033:1034	Mianfen No 1	1025:1036	Mianfen No 1	1025:1036	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	9	54	theme	qualitative	1694:1704	arg1	changes					1716:1722	the qualitative structure changes	1690:1722	the qualitative structure changes	1690:1722	The expressions of IbEXP1, IbEXP2 and IbEXPL1 were significantly correlated with the changes in cell wall component content, and were related to the qualitative structure changes.
32992132	11	55	theme	processing	2040:2049	arg1	changes					2003:2009	texture changes	1995:2009	texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots	1995:2106	This study provides theoretical guidance for in-depth study of texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots.
32992132	8	56	theme	various	1505:1511	arg1	stages					1513:1518	the various stages	1501:1518	the various stages of root tubers expansin	1501:1542	Expansin genes were also expressed differently during the various stages of root tubers expansin.
32992132	8	57	theme	Expansin	1447:1454	arg1	genes					1456:1460	Expansin genes	1447:1460	Expansin genes	1447:1460	Expansin genes were also expressed differently during the various stages of root tubers expansin.
32992132	6	58	theme	Yushu	1147:1151	arg1	No					1153:1154	Yushu No 10	1147:1157	Yushu No 10	1147:1157	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	2	59	theme	change	319:324	arg1	mechanism					326:334	the texture change mechanism	307:334	the texture change mechanism of sweetpotato	307:349	To investigate the texture change mechanism of sweetpotato during the growth process, this study selected two varieties with significant differences in texture from 35 varieties.
32992132	4	60	theme	cell	583:586	arg1	content					605:611	the cell wall composition content	579:611	the cell wall composition content	579:611	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	4	60	theme	cell	583:586	arg1	parameters					567:576	the texture parameters	555:576	the texture parameters	555:576	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	10	61	theme	texture	1764:1770	arg1	related					1817:1823	related	1817:1823	related	1817:1823	The research conclusion shows that the texture changes during the growth of sweetpotato are related to cell wall composition, cell wall-related enzyme activity changes, and the expression of expansin genes.
32992132	10	61	theme	texture	1764:1770	arg1	changes					1772:1778	the texture changes	1760:1778	the texture changes during the growth of sweetpotato	1760:1811	The research conclusion shows that the texture changes during the growth of sweetpotato are related to cell wall composition, cell wall-related enzyme activity changes, and the expression of expansin genes.
32992132	8	62	theme	tubers	1528:1533	arg1	expansin					1535:1542	root tubers expansin	1523:1542	root tubers expansin	1523:1542	Expansin genes were also expressed differently during the various stages of root tubers expansin.
32992132	0	63	theme	polysaccharide-degrading	36:59	arg1	enzymes					61:67	polysaccharide-degrading enzymes	36:67	polysaccharide-degrading enzymes	36:67	Changes in cell wall components and polysaccharide-degrading enzymes in relation to differences in texture during sweetpotato storage root growth.
32992132	9	64	from	changes	1630:1636	arg1	content					1661:1667	cell wall component content	1641:1667	cell wall component content	1641:1667	The expressions of IbEXP1, IbEXP2 and IbEXPL1 were significantly correlated with the changes in cell wall component content, and were related to the qualitative structure changes.
32992132	11	65	theme	in-depth	1977:1984	arg1	study					1986:1990	in-depth study	1977:1990	in-depth study of texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots	1977:2106	This study provides theoretical guidance for in-depth study of texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots.
32992132	7	66	theme	Yushu	1256:1260	arg1	gumminess					1278:1286	'Yushu No 10' hardness gumminess	1255:1286	'Yushu No 10' hardness gumminess	1255:1286	In terms of cell wall-related enzymes, 'Yushu No 10' hardness gumminess and chewiness had a significant correlation with hemicellulose activity, and 'Mianfen No 1' had insignificant correlation with four cell wall-related enzymes.
32992132	11	67	theme	roots	2102:2106	arg1	utilization					2055:2065	utilization	2055:2065	utilization	2055:2065	This study provides theoretical guidance for in-depth study of texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots.
32992132	11	67	theme	roots	2102:2106	arg1	sweetpotato					2014:2024	sweetpotato	2014:2024	sweetpotato	2014:2024	This study provides theoretical guidance for in-depth study of texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots.
32992132	11	67	theme	roots	2102:2106	arg1	processing					2040:2049	post-harvest processing	2027:2049	post-harvest processing	2027:2049	This study provides theoretical guidance for in-depth study of texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots.
32992132	11	67	theme	roots	2102:2106	arg1	quality					2071:2077	quality	2071:2077	quality	2071:2077	This study provides theoretical guidance for in-depth study of texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots.
32992132	6	68	theme	cellulose	1125:1133	arg1	content					1135:1141	the cellulose content	1121:1141	the cellulose content of 'Yushu No 10'	1121:1158	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	6	68	theme	cellulose	1125:1133	arg1	higher					1180:1185	higher	1180:1185	higher	1180:1185	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	5	69	theme	Mianfen	848:854	arg1	No					856:857	Mianfen No 1	848:859	'Mianfen No 1'	847:860	The results show that the hardness, adhesiveness and chewiness parameters of 'Yushu No 10' were significantly lower than those of 'Mianfen No 1', they have significantly different texture properties.
32992132	6	70	theme	composition	939:949	arg1	terms					920:924	terms	920:924	terms of cell wall composition	920:949	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	6	71	theme	pectin	1062:1067	arg1	lower					1081:1085	lower	1081:1085	lower	1081:1085	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	6	71	theme	pectin	1062:1067	arg1	content					1069:1075	the insoluble pectin content	1048:1075	the insoluble pectin content	1048:1075	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	0	72	theme	root	134:137	arg1	growth					139:144	sweetpotato storage root growth	114:144	sweetpotato storage root growth	114:144	Changes in cell wall components and polysaccharide-degrading enzymes in relation to differences in texture during sweetpotato storage root growth.
32992132	6	73	theme	cell	929:932	arg1	composition					939:949	cell wall composition	929:949	cell wall composition	929:949	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	0	74	from	differences	84:94	arg1	texture					99:105	texture	99:105	texture during sweetpotato storage root growth	99:144	Changes in cell wall components and polysaccharide-degrading enzymes in relation to differences in texture during sweetpotato storage root growth.
32992132	7	75	theme	wall-related	1233:1244	arg1	enzymes					1246:1252	cell wall-related enzymes	1228:1252	cell wall-related enzymes	1228:1252	In terms of cell wall-related enzymes, 'Yushu No 10' hardness gumminess and chewiness had a significant correlation with hemicellulose activity, and 'Mianfen No 1' had insignificant correlation with four cell wall-related enzymes.
32992132	4	76	theme	wall-related	619:630	arg1	activities					639:648	cell wall-related enzyme activities	614:648	cell wall-related enzyme activities	614:648	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	4	76	theme	wall-related	619:630	arg1	parameters					567:576	the texture parameters	555:576	the texture parameters	555:576	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	1	77	theme	eating	222:227	arg1	quality					229:235	eating quality	222:235	eating quality	222:235	Sweetpotato has special texture characteristics, which directly affect the eating quality and post-production processing quality of sweetpotato.
32992132	7	78	theme	cell	1420:1423	arg1	enzymes					1438:1444	four cell wall-related enzymes	1415:1444	four cell wall-related enzymes	1415:1444	In terms of cell wall-related enzymes, 'Yushu No 10' hardness gumminess and chewiness had a significant correlation with hemicellulose activity, and 'Mianfen No 1' had insignificant correlation with four cell wall-related enzymes.
32992132	4	79	theme	roots	710:714	arg1	activities					639:648	cell wall-related enzyme activities	614:648	cell wall-related enzyme activities	614:648	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	4	79	theme	roots	710:714	arg1	content					605:611	the cell wall composition content	579:611	the cell wall composition content	579:611	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	4	79	theme	roots	710:714	arg1	parameters					567:576	the texture parameters	555:576	the texture parameters	555:576	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	4	79	theme	roots	710:714	arg1	expression					658:667	the expression	654:667	the expression of expansin genes of sweetpotato storage roots	654:714	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	10	80	theme	sweetpotato	1801:1811	arg1	growth					1791:1796	the growth	1787:1796	the growth of sweetpotato	1787:1811	The research conclusion shows that the texture changes during the growth of sweetpotato are related to cell wall composition, cell wall-related enzyme activity changes, and the expression of expansin genes.
32992132	5	81	theme	different	887:895	arg1	properties					905:914	significantly different texture properties	873:914	significantly different texture properties	873:914	The results show that the hardness, adhesiveness and chewiness parameters of 'Yushu No 10' were significantly lower than those of 'Mianfen No 1', they have significantly different texture properties.
32992132	7	82	theme	cell	1228:1231	arg1	enzymes					1246:1252	cell wall-related enzymes	1228:1252	cell wall-related enzymes	1228:1252	In terms of cell wall-related enzymes, 'Yushu No 10' hardness gumminess and chewiness had a significant correlation with hemicellulose activity, and 'Mianfen No 1' had insignificant correlation with four cell wall-related enzymes.
32992132	6	83	theme	Yushu	983:987	arg1	No					989:990	Yushu No 10	983:993	Yushu No 10	983:993	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	6	84	theme	Mianfen	1101:1107	arg1	No					1109:1110	Mianfen No 1	1101:1112	'Mianfen No 1'	1100:1113	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	7	85	with	correlation	1398:1408	arg1	enzymes					1438:1444	four cell wall-related enzymes	1415:1444	four cell wall-related enzymes	1415:1444	In terms of cell wall-related enzymes, 'Yushu No 10' hardness gumminess and chewiness had a significant correlation with hemicellulose activity, and 'Mianfen No 1' had insignificant correlation with four cell wall-related enzymes.
32992132	7	86	contain	had	1302:1304	arg1	gumminess					1278:1286	'Yushu No 10' hardness gumminess	1255:1286	'Yushu No 10' hardness gumminess	1255:1286	In terms of cell wall-related enzymes, 'Yushu No 10' hardness gumminess and chewiness had a significant correlation with hemicellulose activity, and 'Mianfen No 1' had insignificant correlation with four cell wall-related enzymes.
32992132	7	86	contain	had	1302:1304	arg2	correlation					1320:1330	a significant correlation	1306:1330	a significant correlation with hemicellulose activity	1306:1358	In terms of cell wall-related enzymes, 'Yushu No 10' hardness gumminess and chewiness had a significant correlation with hemicellulose activity, and 'Mianfen No 1' had insignificant correlation with four cell wall-related enzymes.
32992132	7	86	contain	had	1302:1304	arg1	chewiness					1292:1300	chewiness	1292:1300	chewiness	1292:1300	In terms of cell wall-related enzymes, 'Yushu No 10' hardness gumminess and chewiness had a significant correlation with hemicellulose activity, and 'Mianfen No 1' had insignificant correlation with four cell wall-related enzymes.
32992132	7	87	theme	insignificant	1384:1396	arg1	correlation					1398:1408	insignificant correlation	1384:1408	insignificant correlation with four cell wall-related enzymes	1384:1444	In terms of cell wall-related enzymes, 'Yushu No 10' hardness gumminess and chewiness had a significant correlation with hemicellulose activity, and 'Mianfen No 1' had insignificant correlation with four cell wall-related enzymes.
32992132	4	88	theme	genes	681:685	arg1	activities					639:648	cell wall-related enzyme activities	614:648	cell wall-related enzyme activities	614:648	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	4	88	theme	genes	681:685	arg1	content					605:611	the cell wall composition content	579:611	the cell wall composition content	579:611	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	4	88	theme	genes	681:685	arg1	parameters					567:576	the texture parameters	555:576	the texture parameters	555:576	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	4	88	theme	genes	681:685	arg1	expression					658:667	the expression	654:667	the expression of expansin genes of sweetpotato storage roots	654:714	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	10	89	theme	cell	1828:1831	arg1	composition					1838:1848	cell wall composition	1828:1848	cell wall composition	1828:1848	The research conclusion shows that the texture changes during the growth of sweetpotato are related to cell wall composition, cell wall-related enzyme activity changes, and the expression of expansin genes.
32992132	1	90	theme	special	163:169	arg1	characteristics					179:193	special texture characteristics	163:193	special texture characteristics	163:193	Sweetpotato has special texture characteristics, which directly affect the eating quality and post-production processing quality of sweetpotato.
32992132	6	91	theme	pectin	964:969	arg1	more					1000:1003	more	1000:1003	more	1000:1003	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	6	91	theme	pectin	964:969	arg1	content					971:977	the soluble pectin content	952:977	the soluble pectin content of 'Yushu No 10'	952:994	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	4	92	theme	sweetpotato	690:700	arg1	roots					710:714	sweetpotato storage roots	690:714	sweetpotato storage roots	690:714	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	10	93	theme	enzyme	1869:1874	arg1	changes					1885:1891	cell wall-related enzyme activity changes	1851:1891	cell wall-related enzyme activity changes	1851:1891	The research conclusion shows that the texture changes during the growth of sweetpotato are related to cell wall composition, cell wall-related enzyme activity changes, and the expression of expansin genes.
32992132	11	94	theme	storage	2094:2100	arg1	roots					2102:2106	storage roots	2094:2106	storage roots	2094:2106	This study provides theoretical guidance for in-depth study of texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots.
32992132	10	95	theme	cell	1851:1854	arg1	changes					1885:1891	cell wall-related enzyme activity changes	1851:1891	cell wall-related enzyme activity changes	1851:1891	The research conclusion shows that the texture changes during the growth of sweetpotato are related to cell wall composition, cell wall-related enzyme activity changes, and the expression of expansin genes.
32992132	0	96	theme	sweetpotato	114:124	arg1	growth					139:144	sweetpotato storage root growth	114:144	sweetpotato storage root growth	114:144	Changes in cell wall components and polysaccharide-degrading enzymes in relation to differences in texture during sweetpotato storage root growth.
32992132	2	97	theme	significant	417:427	arg1	differences					429:439	significant differences	417:439	significant differences in texture from 35 varieties	417:468	To investigate the texture change mechanism of sweetpotato during the growth process, this study selected two varieties with significant differences in texture from 35 varieties.
32992132	5	98	theme	No	801:802	arg1	parameters					780:789	the hardness, adhesiveness and chewiness parameters	739:789	the hardness, adhesiveness and chewiness parameters of 'Yushu No 10'	739:806	The results show that the hardness, adhesiveness and chewiness parameters of 'Yushu No 10' were significantly lower than those of 'Mianfen No 1', they have significantly different texture properties.
32992132	5	98	theme	No	801:802	arg1	lower					827:831	lower	827:831	lower	827:831	The results show that the hardness, adhesiveness and chewiness parameters of 'Yushu No 10' were significantly lower than those of 'Mianfen No 1', they have significantly different texture properties.
32992132	11	99	theme	changes	2003:2009	arg1	study					1986:1990	in-depth study	1977:1990	in-depth study of texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots	1977:2106	This study provides theoretical guidance for in-depth study of texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots.
32992132	0	100	theme	wall	16:19	arg1	components					21:30	cell wall components	11:30	cell wall components	11:30	Changes in cell wall components and polysaccharide-degrading enzymes in relation to differences in texture during sweetpotato storage root growth.
32992132	9	101	theme	wall	1646:1649	arg1	content					1661:1667	cell wall component content	1641:1667	cell wall component content	1641:1667	The expressions of IbEXP1, IbEXP2 and IbEXPL1 were significantly correlated with the changes in cell wall component content, and were related to the qualitative structure changes.
32992132	7	102	theme	Mianfen	1366:1372	arg1	No					1374:1375	Mianfen No 1	1366:1377	'Mianfen No 1'	1365:1378	In terms of cell wall-related enzymes, 'Yushu No 10' hardness gumminess and chewiness had a significant correlation with hemicellulose activity, and 'Mianfen No 1' had insignificant correlation with four cell wall-related enzymes.
32992132	11	103	theme	post-harvest	2027:2038	arg1	processing					2040:2049	post-harvest processing	2027:2049	post-harvest processing	2027:2049	This study provides theoretical guidance for in-depth study of texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots.
32992132	6	104	theme	Mianfen	1201:1207	arg1	No					1209:1210	Mianfen No 1	1201:1212	'Mianfen No 1'	1200:1213	In terms of cell wall composition, the soluble pectin content of 'Yushu No 10' was more than twice that of 'Mianfen No 1', whereas the insoluble pectin content was lower than that of 'Mianfen No 1', with the cellulose content of 'Yushu No 10' being significantly higher than that of 'Mianfen No 1'.
32992132	7	105	contain	had	1380:1382	arg1	No					1374:1375	Mianfen No 1	1366:1377	'Mianfen No 1'	1365:1378	In terms of cell wall-related enzymes, 'Yushu No 10' hardness gumminess and chewiness had a significant correlation with hemicellulose activity, and 'Mianfen No 1' had insignificant correlation with four cell wall-related enzymes.
32992132	7	105	contain	had	1380:1382	arg2	correlation					1398:1408	insignificant correlation	1384:1408	insignificant correlation with four cell wall-related enzymes	1384:1444	In terms of cell wall-related enzymes, 'Yushu No 10' hardness gumminess and chewiness had a significant correlation with hemicellulose activity, and 'Mianfen No 1' had insignificant correlation with four cell wall-related enzymes.
32992132	11	106	theme	sweetpotato	2014:2024	arg1	changes					2003:2009	texture changes	1995:2009	texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots	1995:2106	This study provides theoretical guidance for in-depth study of texture changes of sweetpotato, post-harvest processing and utilization and quality improvement of storage roots.
32992132	1	107	dep	quality	229:235	arg1	the					218:220	the	218:220	the	218:220	Sweetpotato has special texture characteristics, which directly affect the eating quality and post-production processing quality of sweetpotato.
32992132	1	108	theme	post-production	241:255	arg1	quality					268:274	post-production processing quality	241:274	post-production processing quality	241:274	Sweetpotato has special texture characteristics, which directly affect the eating quality and post-production processing quality of sweetpotato.
32992132	10	109	theme	genes	1925:1929	arg1	composition					1838:1848	cell wall composition	1828:1848	cell wall composition	1828:1848	The research conclusion shows that the texture changes during the growth of sweetpotato are related to cell wall composition, cell wall-related enzyme activity changes, and the expression of expansin genes.
32992132	10	109	theme	genes	1925:1929	arg1	changes					1885:1891	cell wall-related enzyme activity changes	1851:1891	cell wall-related enzyme activity changes	1851:1891	The research conclusion shows that the texture changes during the growth of sweetpotato are related to cell wall composition, cell wall-related enzyme activity changes, and the expression of expansin genes.
32992132	10	109	theme	genes	1925:1929	arg1	expression					1902:1911	the expression	1898:1911	the expression of expansin genes	1898:1929	The research conclusion shows that the texture changes during the growth of sweetpotato are related to cell wall composition, cell wall-related enzyme activity changes, and the expression of expansin genes.
32992132	3	110	theme	storage	475:481	arg1	roots					483:487	The storage roots	471:487	The storage roots	471:487	The storage roots were sampled at 50, 80, 110, and 140 days after planting.
32992132	4	111	theme	cell	614:617	arg1	activities					639:648	cell wall-related enzyme activities	614:648	cell wall-related enzyme activities	614:648	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
32992132	4	111	theme	cell	614:617	arg1	parameters					567:576	the texture parameters	555:576	the texture parameters	555:576	Measure the texture parameters, the cell wall composition content, cell wall-related enzyme activities and the expression of expansin genes of sweetpotato storage roots.
33415749	5	0	dep	predict	927:933	arg1	inside					969:974	inside	969:974	inside the HP-β-CD cavity and the optimal stoichiometry of the complex formation	969:1048	Molecular simulations are applied to predict the possible orientations of SAEE inside the HP-β-CD cavity and the optimal stoichiometry of the complex formation.
33415749	7	1	theme	loss	1335:1338	arg1	rate					1340:1343	weight loss rate	1328:1343	weight loss rate of fresh-cut Chinese yam	1328:1368	In addition, the composite films can maintain high-content vitamin C and reduce weight loss rate of fresh-cut Chinese yam.
33415749	3	2	theme	characterizations	567:583	arg1	terms					518:522	terms	518:522	terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations	518:746	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	3	3	theme	microstructure	552:565	arg1	microscopy					618:627	transmission electron microscopy	596:627	transmission electron microscopy	596:627	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	3	3	theme	microstructure	552:565	arg1	characterizations					567:583	microstructure characterizations	552:583	microstructure characterizations	552:583	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	3	3	theme	microstructure	552:565	arg1	Fourier					662:668	Fourier	662:668	Fourier transform infrared	662:687	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	3	3	theme	microstructure	552:565	arg1	microscopy					650:659	scanning electronic microscopy	630:659	scanning electronic microscopy	630:659	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	3	3	theme	microstructure	552:565	arg1	analysis					702:709	1 H NMR analysis	694:709	1 H NMR analysis	694:709	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	12	4	theme	alginate	2138:2145	arg1	molecules					2147:2155	sodium alginate molecules	2131:2155	sodium alginate molecules	2131:2155	It can serve as a powerful tool to calculate the energy of association between inclusion complex and sodium alginate molecules.
33415749	10	5	theme	anise	1769:1773	arg1	complex					1847:1853	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	1764:1853	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation	1764:1903	PRACTICAL APPLICATION: In this paper, we present composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation.
33415749	3	6	theme	electron	609:616	arg1	microscopy					618:627	transmission electron microscopy	596:627	transmission electron microscopy	596:627	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	1	7	theme	composite	200:208	arg1	films					210:214	composite films	200:214	composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	200:323	The aim of this study is to prepare composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex.
33415749	12	8	theme	association	2089:2099	arg1	energy					2079:2084	the energy	2075:2084	the energy of association between inclusion complex and sodium alginate molecules	2075:2155	It can serve as a powerful tool to calculate the energy of association between inclusion complex and sodium alginate molecules.
33415749	2	9	theme	sodium	341:346	arg1	concentration					357:369	sodium alginate concentration	341:369	sodium alginate concentration	341:369	The effects of sodium alginate concentration on mechanical properties of films are tested.
33415749	9	10	theme	composite	1588:1596	arg1	films					1598:1602	The composite films	1584:1602	The composite films in this study	1584:1616	The composite films in this study have the potential to be used for food packaging applications.
33415749	12	11	theme	inclusion	2109:2117	arg1	complex					2119:2125	inclusion complex	2109:2125	inclusion complex	2109:2125	It can serve as a powerful tool to calculate the energy of association between inclusion complex and sodium alginate molecules.
33415749	10	12	dep	APPLICATION	1691:1701	arg1	present					1722:1728	present	1722:1728	present composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation	1722:1903	PRACTICAL APPLICATION: In this paper, we present composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation.
33415749	3	13	theme	electronic	639:648	arg1	microscopy					650:659	scanning electronic microscopy	630:659	scanning electronic microscopy	630:659	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	1	14	theme	star	234:237	arg1	complex					317:323	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	234:323	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	234:323	The aim of this study is to prepare composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex.
33415749	8	15	theme	complex	1575:1581	arg1	structure					1500:1508	reasonable molecular structure	1479:1508	reasonable molecular structure	1479:1508	In conclusion, coinciding with the experimental results, the molecular modeling successfully calculates the reasonable molecular structure and molecular behavior of sodium alginate/SAEE/HP-β-CD inclusion complex.
33415749	8	15	theme	complex	1575:1581	arg1	behavior					1524:1531	molecular behavior	1514:1531	molecular behavior	1514:1531	In conclusion, coinciding with the experimental results, the molecular modeling successfully calculates the reasonable molecular structure and molecular behavior of sodium alginate/SAEE/HP-β-CD inclusion complex.
33415749	10	16	theme	star	1764:1767	arg1	complex					1847:1853	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	1764:1853	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation	1764:1903	PRACTICAL APPLICATION: In this paper, we present composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation.
33415749	11	17	theme	molecular	1965:1973	arg1	modeling					1975:1982	molecular modeling	1965:1982	molecular modeling	1965:1982	The present study demonstrates a successful application of molecular modeling to predict the geometry of the final complex.
33415749	5	18	theme	SAEE	964:967	arg1	orientations					948:959	the possible orientations	935:959	the possible orientations of SAEE	935:967	Molecular simulations are applied to predict the possible orientations of SAEE inside the HP-β-CD cavity and the optimal stoichiometry of the complex formation.
33415749	10	19	theme	composite	1730:1738	arg1	films					1740:1744	composite films	1730:1744	composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation	1730:1903	PRACTICAL APPLICATION: In this paper, we present composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation.
33415749	4	20	theme	alginate/SAEE/HP-β-CD	803:823	arg1	complexes					783:791	the complexes	779:791	the complexes of sodium alginate/SAEE/HP-β-CD	779:823	With various stoichiometries, the complexes of sodium alginate/SAEE/HP-β-CD are compared through both theoretical and experimental analyses.
33415749	3	21	theme	NMR	698:700	arg1	analysis					702:709	1 H NMR analysis	694:709	1 H NMR analysis	694:709	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	5	22	theme	formation	1040:1048	arg1	stoichiometry					1011:1023	the optimal stoichiometry	999:1023	the optimal stoichiometry of the complex formation	999:1048	Molecular simulations are applied to predict the possible orientations of SAEE inside the HP-β-CD cavity and the optimal stoichiometry of the complex formation.
33415749	5	22	theme	formation	1040:1048	arg1	cavity					988:993	the HP-β-CD cavity	976:993	the HP-β-CD cavity	976:993	Molecular simulations are applied to predict the possible orientations of SAEE inside the HP-β-CD cavity and the optimal stoichiometry of the complex formation.
33415749	6	23	theme	inclusion	1132:1140	arg1	alginate/SAEE					1101:1113	sodium alginate/SAEE	1094:1113	sodium alginate/SAEE (or SAEE/HP-β-CD inclusion complex)	1094:1149	According to the simulation, the system of sodium alginate/SAEE (or SAEE/HP-β-CD inclusion complex) in a 3:1 stoichiometry reaches the lowest total energy and achieves a balance in complex system.
33415749	6	23	theme	inclusion	1132:1140	arg1	complex					1142:1148	SAEE/HP-β-CD inclusion complex	1119:1148	SAEE/HP-β-CD inclusion complex	1119:1148	According to the simulation, the system of sodium alginate/SAEE (or SAEE/HP-β-CD inclusion complex) in a 3:1 stoichiometry reaches the lowest total energy and achieves a balance in complex system.
33415749	8	24	theme	experimental	1406:1417	arg1	results					1419:1425	the experimental results	1402:1425	the experimental results	1402:1425	In conclusion, coinciding with the experimental results, the molecular modeling successfully calculates the reasonable molecular structure and molecular behavior of sodium alginate/SAEE/HP-β-CD inclusion complex.
33415749	7	25	theme	weight	1328:1333	arg1	loss					1335:1338	weight loss	1328:1338	weight loss rate of fresh-cut Chinese yam	1328:1368	In addition, the composite films can maintain high-content vitamin C and reduce weight loss rate of fresh-cut Chinese yam.
33415749	3	26	theme	1	694:694	arg1	H					696:696	H	696:696	H	696:696	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	1	27	theme	study	180:184	arg1	aim					168:170	The aim	164:170	The aim of this study	164:184	The aim of this study is to prepare composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex.
33415749	0	28	theme	star	89:92	arg1	complex					155:161	star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex	89:161	star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex	89:161	Theoretical and experimental investigation of sodium alginate composite films containing star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex.
33415749	8	29	dep	structure	1500:1508	arg1	the					1475:1477	the	1475:1477	the	1475:1477	In conclusion, coinciding with the experimental results, the molecular modeling successfully calculates the reasonable molecular structure and molecular behavior of sodium alginate/SAEE/HP-β-CD inclusion complex.
33415749	6	30	theme	alginate/SAEE	1101:1113	arg1	system					1084:1089	the system	1080:1089	the system of sodium alginate/SAEE (or SAEE/HP-β-CD inclusion complex) in a 3:1 stoichiometry	1080:1172	According to the simulation, the system of sodium alginate/SAEE (or SAEE/HP-β-CD inclusion complex) in a 3:1 stoichiometry reaches the lowest total energy and achieves a balance in complex system.
33415749	10	31	attach	present	1722:1728	arg1	paper					1712:1716	this paper	1707:1716	this paper	1707:1716	PRACTICAL APPLICATION: In this paper, we present composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation.
33415749	10	31	attach	present	1722:1728	arg2	we					1719:1720	we	1719:1720	we	1719:1720	PRACTICAL APPLICATION: In this paper, we present composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation.
33415749	3	32	dep	alginate	424:431	arg1	films					491:495	inclusion complex-based composite films	457:495	inclusion complex-based composite films	457:495	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	8	33	theme	molecular	1432:1440	arg1	modeling					1442:1449	the molecular modeling	1428:1449	the molecular modeling	1428:1449	In conclusion, coinciding with the experimental results, the molecular modeling successfully calculates the reasonable molecular structure and molecular behavior of sodium alginate/SAEE/HP-β-CD inclusion complex.
33415749	0	34	theme	ethanol	100:106	arg1	complex					155:161	star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex	89:161	star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex	89:161	Theoretical and experimental investigation of sodium alginate composite films containing star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex.
33415749	10	35	theme	Chinese	1880:1886	arg1	preservation					1892:1903	fresh-cut Chinese yam preservation	1870:1903	fresh-cut Chinese yam preservation	1870:1903	PRACTICAL APPLICATION: In this paper, we present composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation.
33415749	3	36	theme	composite	481:489	arg1	films					491:495	inclusion complex-based composite films	457:495	inclusion complex-based composite films	457:495	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	0	37	theme	inclusion	145:153	arg1	complex					155:161	star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex	89:161	star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex	89:161	Theoretical and experimental investigation of sodium alginate composite films containing star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex.
33415749	3	38	theme	inclusion	457:465	arg1	films					491:495	inclusion complex-based composite films	457:495	inclusion complex-based composite films	457:495	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	0	39	theme	Theoretical	0:10	arg1	investigation					29:41	Theoretical and experimental investigation	0:41	Theoretical and experimental investigation of sodium alginate composite films	0:76	Theoretical and experimental investigation of sodium alginate composite films containing star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex.
33415749	1	40	theme	inclusion	307:315	arg1	complex					317:323	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	234:323	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	234:323	The aim of this study is to prepare composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex.
33415749	8	41	theme	molecular	1490:1498	arg1	structure					1500:1508	reasonable molecular structure	1479:1508	reasonable molecular structure	1479:1508	In conclusion, coinciding with the experimental results, the molecular modeling successfully calculates the reasonable molecular structure and molecular behavior of sodium alginate/SAEE/HP-β-CD inclusion complex.
33415749	10	42	theme	/hydroxypropyl-β-cyclodextrin	1797:1825	arg1	complex					1847:1853	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	1764:1853	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation	1764:1903	PRACTICAL APPLICATION: In this paper, we present composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation.
33415749	0	43	theme	experimental	16:27	arg1	investigation					29:41	Theoretical and experimental investigation	0:41	Theoretical and experimental investigation of sodium alginate composite films	0:76	Theoretical and experimental investigation of sodium alginate composite films containing star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex.
33415749	10	44	theme	fresh-cut	1870:1878	arg1	preservation					1892:1903	fresh-cut Chinese yam preservation	1870:1903	fresh-cut Chinese yam preservation	1870:1903	PRACTICAL APPLICATION: In this paper, we present composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation.
33415749	6	45	from	system	1084:1089	arg1	stoichiometry					1160:1172	a 3:1 stoichiometry	1154:1172	a 3:1 stoichiometry	1154:1172	According to the simulation, the system of sodium alginate/SAEE (or SAEE/HP-β-CD inclusion complex) in a 3:1 stoichiometry reaches the lowest total energy and achieves a balance in complex system.
33415749	3	46	theme	molecular	716:724	arg1	modeling					726:733	molecular modeling	716:733	molecular modeling calculations	716:746	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	10	47	theme	SAEE	1792:1795	arg1	complex					1847:1853	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	1764:1853	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation	1764:1903	PRACTICAL APPLICATION: In this paper, we present composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation.
33415749	5	48	theme	Molecular	890:898	arg1	simulations					900:910	Molecular simulations	890:910	Molecular simulations	890:910	Molecular simulations are applied to predict the possible orientations of SAEE inside the HP-β-CD cavity and the optimal stoichiometry of the complex formation.
33415749	0	49	theme	films	72:76	arg1	investigation					29:41	Theoretical and experimental investigation	0:41	Theoretical and experimental investigation of sodium alginate composite films	0:76	Theoretical and experimental investigation of sodium alginate composite films containing star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex.
33415749	3	50	theme	spectroscopy	538:549	arg1	terms					518:522	terms	518:522	terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations	518:746	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	0	51	theme	alginate	53:60	arg1	films					72:76	sodium alginate composite films	46:76	sodium alginate composite films	46:76	Theoretical and experimental investigation of sodium alginate composite films containing star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex.
33415749	9	52	from	films	1598:1602	arg1	study					1612:1616	this study	1607:1616	this study	1607:1616	The composite films in this study have the potential to be used for food packaging applications.
33415749	8	53	theme	sodium	1536:1541	arg1	complex					1575:1581	sodium alginate/SAEE/HP-β-CD inclusion complex	1536:1581	sodium alginate/SAEE/HP-β-CD inclusion complex	1536:1581	In conclusion, coinciding with the experimental results, the molecular modeling successfully calculates the reasonable molecular structure and molecular behavior of sodium alginate/SAEE/HP-β-CD inclusion complex.
33415749	10	54	theme	HP-β-CD	1828:1834	arg1	complex					1847:1853	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	1764:1853	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation	1764:1903	PRACTICAL APPLICATION: In this paper, we present composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation.
33415749	1	55	theme	extract	253:259	arg1	complex					317:323	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	234:323	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	234:323	The aim of this study is to prepare composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex.
33415749	7	56	theme	yam	1366:1368	arg1	rate					1340:1343	weight loss rate	1328:1343	weight loss rate of fresh-cut Chinese yam	1328:1368	In addition, the composite films can maintain high-content vitamin C and reduce weight loss rate of fresh-cut Chinese yam.
33415749	10	57	theme	PRACTICAL	1681:1689	arg1	APPLICATION					1691:1701	PRACTICAL APPLICATION	1681:1701	PRACTICAL APPLICATION: In this paper, we present composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation.	1681:1904	PRACTICAL APPLICATION: In this paper, we present composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation.
33415749	3	58	theme	calculations	735:746	arg1	terms					518:522	terms	518:522	terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations	518:746	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	8	59	theme	inclusion	1565:1573	arg1	complex					1575:1581	sodium alginate/SAEE/HP-β-CD inclusion complex	1536:1581	sodium alginate/SAEE/HP-β-CD inclusion complex	1536:1581	In conclusion, coinciding with the experimental results, the molecular modeling successfully calculates the reasonable molecular structure and molecular behavior of sodium alginate/SAEE/HP-β-CD inclusion complex.
33415749	11	60	theme	final	2015:2019	arg1	complex					2021:2027	the final complex	2011:2027	the final complex	2011:2027	The present study demonstrates a successful application of molecular modeling to predict the geometry of the final complex.
33415749	3	61	dep	transform	670:678	arg1	infrared					680:687	infrared	680:687	transform infrared	670:687	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	1	62	theme	SAEE	262:265	arg1	complex					317:323	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	234:323	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	234:323	The aim of this study is to prepare composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex.
33415749	7	63	theme	fresh-cut	1348:1356	arg1	yam					1366:1368	fresh-cut Chinese yam	1348:1368	fresh-cut Chinese yam	1348:1368	In addition, the composite films can maintain high-content vitamin C and reduce weight loss rate of fresh-cut Chinese yam.
33415749	2	64	theme	films	399:403	arg1	properties					385:394	mechanical properties	374:394	mechanical properties of films	374:403	The effects of sodium alginate concentration on mechanical properties of films are tested.
33415749	1	65	theme	/hydroxypropyl-β-cyclodextrin	267:295	arg1	complex					317:323	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	234:323	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	234:323	The aim of this study is to prepare composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex.
33415749	6	66	theme	total	1193:1197	arg1	energy					1199:1204	the lowest total energy	1182:1204	the lowest total energy	1182:1204	According to the simulation, the system of sodium alginate/SAEE (or SAEE/HP-β-CD inclusion complex) in a 3:1 stoichiometry reaches the lowest total energy and achieves a balance in complex system.
33415749	10	67	theme	yam	1888:1890	arg1	preservation					1892:1903	fresh-cut Chinese yam preservation	1870:1903	fresh-cut Chinese yam preservation	1870:1903	PRACTICAL APPLICATION: In this paper, we present composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation.
33415749	1	68	theme	HP-β-CD	298:304	arg1	complex					317:323	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	234:323	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	234:323	The aim of this study is to prepare composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex.
33415749	10	69	theme	inclusion	1837:1845	arg1	complex					1847:1853	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	1764:1853	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation	1764:1903	PRACTICAL APPLICATION: In this paper, we present composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation.
33415749	3	70	theme	transmission	596:607	arg1	microscopy					618:627	transmission electron microscopy	596:627	transmission electron microscopy	596:627	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	3	71	dep	Fourier	662:668	arg1	transform					670:678	transform	670:678	transform infrared	670:687	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	12	72	theme	sodium	2131:2136	arg1	molecules					2147:2155	sodium alginate molecules	2131:2155	sodium alginate molecules	2131:2155	It can serve as a powerful tool to calculate the energy of association between inclusion complex and sodium alginate molecules.
33415749	2	73	theme	alginate	348:355	arg1	concentration					357:369	sodium alginate concentration	341:369	sodium alginate concentration	341:369	The effects of sodium alginate concentration on mechanical properties of films are tested.
33415749	5	74	theme	HP-β-CD	980:986	arg1	cavity					988:993	the HP-β-CD cavity	976:993	the HP-β-CD cavity	976:993	Molecular simulations are applied to predict the possible orientations of SAEE inside the HP-β-CD cavity and the optimal stoichiometry of the complex formation.
33415749	11	75	theme	successful	1939:1948	arg1	application					1950:1960	a successful application	1937:1960	a successful application of molecular modeling to predict the geometry of the final complex	1937:2027	The present study demonstrates a successful application of molecular modeling to predict the geometry of the final complex.
33415749	3	76	theme	scanning	630:637	arg1	microscopy					650:659	scanning electronic microscopy	630:659	scanning electronic microscopy	630:659	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	5	77	theme	possible	939:946	arg1	orientations					948:959	the possible orientations	935:959	the possible orientations of SAEE	935:967	Molecular simulations are applied to predict the possible orientations of SAEE inside the HP-β-CD cavity and the optimal stoichiometry of the complex formation.
33415749	9	78	theme	food	1652:1655	arg1	applications					1667:1678	food packaging applications	1652:1678	food packaging applications	1652:1678	The composite films in this study have the potential to be used for food packaging applications.
33415749	1	79	theme	anise	239:243	arg1	complex					317:323	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	234:323	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	234:323	The aim of this study is to prepare composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex.
33415749	7	80	theme	high-content	1294:1305	arg1	C					1315:1315	high-content vitamin C	1294:1315	high-content vitamin C	1294:1315	In addition, the composite films can maintain high-content vitamin C and reduce weight loss rate of fresh-cut Chinese yam.
33415749	3	81	theme	H	696:696	arg1	analysis					702:709	1 H NMR analysis	694:709	1 H NMR analysis	694:709	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	5	82	theme	complex	1032:1038	arg1	formation					1040:1048	the complex formation	1028:1048	the complex formation	1028:1048	Molecular simulations are applied to predict the possible orientations of SAEE inside the HP-β-CD cavity and the optimal stoichiometry of the complex formation.
33415749	4	83	theme	sodium	796:801	arg1	alginate/SAEE/HP-β-CD					803:823	sodium alginate/SAEE/HP-β-CD	796:823	sodium alginate/SAEE/HP-β-CD	796:823	With various stoichiometries, the complexes of sodium alginate/SAEE/HP-β-CD are compared through both theoretical and experimental analyses.
33415749	6	84	theme	SAEE/HP-β-CD	1119:1130	arg1	alginate/SAEE					1101:1113	sodium alginate/SAEE	1094:1113	sodium alginate/SAEE (or SAEE/HP-β-CD inclusion complex)	1094:1149	According to the simulation, the system of sodium alginate/SAEE (or SAEE/HP-β-CD inclusion complex) in a 3:1 stoichiometry reaches the lowest total energy and achieves a balance in complex system.
33415749	6	84	theme	SAEE/HP-β-CD	1119:1130	arg1	complex					1142:1148	SAEE/HP-β-CD inclusion complex	1119:1148	SAEE/HP-β-CD inclusion complex	1119:1148	According to the simulation, the system of sodium alginate/SAEE (or SAEE/HP-β-CD inclusion complex) in a 3:1 stoichiometry reaches the lowest total energy and achieves a balance in complex system.
33415749	4	85	theme	various	754:760	arg1	stoichiometries					762:776	various stoichiometries	754:776	various stoichiometries	754:776	With various stoichiometries, the complexes of sodium alginate/SAEE/HP-β-CD are compared through both theoretical and experimental analyses.
33415749	7	86	theme	vitamin	1307:1313	arg1	C					1315:1315	high-content vitamin C	1294:1315	high-content vitamin C	1294:1315	In addition, the composite films can maintain high-content vitamin C and reduce weight loss rate of fresh-cut Chinese yam.
33415749	0	87	theme	anise	94:98	arg1	complex					155:161	star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex	89:161	star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex	89:161	Theoretical and experimental investigation of sodium alginate composite films containing star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex.
33415749	5	88	theme	optimal	1003:1009	arg1	stoichiometry					1011:1023	the optimal stoichiometry	999:1023	the optimal stoichiometry of the complex formation	999:1048	Molecular simulations are applied to predict the possible orientations of SAEE inside the HP-β-CD cavity and the optimal stoichiometry of the complex formation.
33415749	2	89	from	effects	330:336	arg1	properties					385:394	mechanical properties	374:394	mechanical properties of films	374:403	The effects of sodium alginate concentration on mechanical properties of films are tested.
33415749	12	90	theme	powerful	2048:2055	arg1	It					2030:2031	It	2030:2031	It	2030:2031	It can serve as a powerful tool to calculate the energy of association between inclusion complex and sodium alginate molecules.
33415749	12	90	theme	powerful	2048:2055	arg1	tool					2057:2060	a powerful tool	2046:2060	a powerful tool to calculate the energy of association between inclusion complex and sodium alginate molecules	2046:2155	It can serve as a powerful tool to calculate the energy of association between inclusion complex and sodium alginate molecules.
33415749	11	91	theme	present	1910:1916	arg1	study					1918:1922	The present study	1906:1922	The present study	1906:1922	The present study demonstrates a successful application of molecular modeling to predict the geometry of the final complex.
33415749	0	92	theme	extract/hydroxypropyl-β-cyclodextrin	108:143	arg1	complex					155:161	star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex	89:161	star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex	89:161	Theoretical and experimental investigation of sodium alginate composite films containing star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex.
33415749	9	93	theme	packaging	1657:1665	arg1	applications					1667:1678	food packaging applications	1652:1678	food packaging applications	1652:1678	The composite films in this study have the potential to be used for food packaging applications.
33415749	10	94	theme	preservation	1892:1903	arg1	use					1863:1865	the use	1859:1865	the use of fresh-cut Chinese yam preservation	1859:1903	PRACTICAL APPLICATION: In this paper, we present composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation.
33415749	6	95	theme	sodium	1094:1099	arg1	alginate/SAEE					1101:1113	sodium alginate/SAEE	1094:1113	sodium alginate/SAEE (or SAEE/HP-β-CD inclusion complex)	1094:1149	According to the simulation, the system of sodium alginate/SAEE (or SAEE/HP-β-CD inclusion complex) in a 3:1 stoichiometry reaches the lowest total energy and achieves a balance in complex system.
33415749	6	95	theme	sodium	1094:1099	arg1	complex					1142:1148	SAEE/HP-β-CD inclusion complex	1119:1148	SAEE/HP-β-CD inclusion complex	1119:1148	According to the simulation, the system of sodium alginate/SAEE (or SAEE/HP-β-CD inclusion complex) in a 3:1 stoichiometry reaches the lowest total energy and achieves a balance in complex system.
33415749	4	96	theme	theoretical	851:861	arg1	analyses					880:887	both theoretical and experimental analyses	846:887	both theoretical and experimental analyses	846:887	With various stoichiometries, the complexes of sodium alginate/SAEE/HP-β-CD are compared through both theoretical and experimental analyses.
33415749	2	97	theme	concentration	357:369	arg1	effects					330:336	The effects	326:336	The effects of sodium alginate concentration on mechanical properties of films	326:403	The effects of sodium alginate concentration on mechanical properties of films are tested.
33415749	6	98	theme	lowest	1186:1191	arg1	energy					1199:1204	the lowest total energy	1182:1204	the lowest total energy	1182:1204	According to the simulation, the system of sodium alginate/SAEE (or SAEE/HP-β-CD inclusion complex) in a 3:1 stoichiometry reaches the lowest total energy and achieves a balance in complex system.
33415749	4	99	theme	experimental	867:878	arg1	analyses					880:887	both theoretical and experimental analyses	846:887	both theoretical and experimental analyses	846:887	With various stoichiometries, the complexes of sodium alginate/SAEE/HP-β-CD are compared through both theoretical and experimental analyses.
33415749	10	100	theme	ethanol	1775:1781	arg1	complex					1847:1853	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	1764:1853	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation	1764:1903	PRACTICAL APPLICATION: In this paper, we present composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation.
33415749	7	101	theme	composite	1265:1273	arg1	films					1275:1279	the composite films	1261:1279	the composite films	1261:1279	In addition, the composite films can maintain high-content vitamin C and reduce weight loss rate of fresh-cut Chinese yam.
33415749	3	102	theme	complex-based	467:479	arg1	films					491:495	inclusion complex-based composite films	457:495	inclusion complex-based composite films	457:495	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	6	103	theme	3:1	1156:1158	arg1	stoichiometry					1160:1172	a 3:1 stoichiometry	1154:1172	a 3:1 stoichiometry	1154:1172	According to the simulation, the system of sodium alginate/SAEE (or SAEE/HP-β-CD inclusion complex) in a 3:1 stoichiometry reaches the lowest total energy and achieves a balance in complex system.
33415749	3	104	theme	Sodium	417:422	arg1	alginate					424:431	Sodium alginate	417:431	Sodium alginate	417:431	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	8	105	theme	reasonable	1479:1488	arg1	structure					1500:1508	reasonable molecular structure	1479:1508	reasonable molecular structure	1479:1508	In conclusion, coinciding with the experimental results, the molecular modeling successfully calculates the reasonable molecular structure and molecular behavior of sodium alginate/SAEE/HP-β-CD inclusion complex.
33415749	0	106	theme	sodium	46:51	arg1	films					72:76	sodium alginate composite films	46:76	sodium alginate composite films	46:76	Theoretical and experimental investigation of sodium alginate composite films containing star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex.
33415749	10	107	theme	extract	1783:1789	arg1	complex					1847:1853	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	1764:1853	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation	1764:1903	PRACTICAL APPLICATION: In this paper, we present composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex for the use of fresh-cut Chinese yam preservation.
33415749	9	108	contain	have	1618:1621	arg1	films					1598:1602	The composite films	1584:1602	The composite films in this study	1584:1616	The composite films in this study have the potential to be used for food packaging applications.
33415749	9	108	contain	have	1618:1621	arg2	potential					1627:1635	the potential to be used for food packaging applications	1623:1678	the potential to be used for food packaging applications	1623:1678	The composite films in this study have the potential to be used for food packaging applications.
33415749	6	109	from	balance	1221:1227	arg1	system					1240:1245	complex system	1232:1245	complex system	1232:1245	According to the simulation, the system of sodium alginate/SAEE (or SAEE/HP-β-CD inclusion complex) in a 3:1 stoichiometry reaches the lowest total energy and achieves a balance in complex system.
33415749	0	110	theme	composite	62:70	arg1	films					72:76	sodium alginate composite films	46:76	sodium alginate composite films	46:76	Theoretical and experimental investigation of sodium alginate composite films containing star anise ethanol extract/hydroxypropyl-β-cyclodextrin inclusion complex.
33415749	6	111	theme	complex	1232:1238	arg1	system					1240:1245	complex system	1232:1245	complex system	1232:1245	According to the simulation, the system of sodium alginate/SAEE (or SAEE/HP-β-CD inclusion complex) in a 3:1 stoichiometry reaches the lowest total energy and achieves a balance in complex system.
33415749	8	112	theme	molecular	1514:1522	arg1	behavior					1524:1531	molecular behavior	1514:1531	molecular behavior	1514:1531	In conclusion, coinciding with the experimental results, the molecular modeling successfully calculates the reasonable molecular structure and molecular behavior of sodium alginate/SAEE/HP-β-CD inclusion complex.
33415749	2	113	theme	mechanical	374:383	arg1	properties					385:394	mechanical properties	374:394	mechanical properties of films	374:403	The effects of sodium alginate concentration on mechanical properties of films are tested.
33415749	7	114	theme	Chinese	1358:1364	arg1	yam					1366:1368	fresh-cut Chinese yam	1348:1368	fresh-cut Chinese yam	1348:1368	In addition, the composite films can maintain high-content vitamin C and reduce weight loss rate of fresh-cut Chinese yam.
33415749	3	115	theme	modeling	726:733	arg1	microscopy					618:627	transmission electron microscopy	596:627	transmission electron microscopy	596:627	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	3	115	theme	modeling	726:733	arg1	calculations					735:746	molecular modeling calculations	716:746	molecular modeling calculations	716:746	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	3	115	theme	modeling	726:733	arg1	Fourier					662:668	Fourier	662:668	Fourier transform infrared	662:687	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	3	115	theme	modeling	726:733	arg1	microscopy					650:659	scanning electronic microscopy	630:659	scanning electronic microscopy	630:659	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	3	115	theme	modeling	726:733	arg1	analysis					702:709	1 H NMR analysis	694:709	1 H NMR analysis	694:709	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	3	116	theme	UV-visible	527:536	arg1	spectroscopy					538:549	UV-visible spectroscopy	527:549	UV-visible spectroscopy	527:549	Sodium alginate, SAEE, and SAEE/HP-β-CD inclusion complex-based composite films are characterized in terms of UV-visible spectroscopy, microstructure characterizations, including transmission electron microscopy, scanning electronic microscopy, Fourier transform infrared, and 1 H NMR analysis, and molecular modeling calculations.
33415749	1	117	theme	ethanol	245:251	arg1	complex					317:323	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	234:323	star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex	234:323	The aim of this study is to prepare composite films incorporated with star anise ethanol extract (SAEE)/hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion complex.
33415749	8	118	theme	alginate/SAEE/HP-β-CD	1543:1563	arg1	complex					1575:1581	sodium alginate/SAEE/HP-β-CD inclusion complex	1536:1581	sodium alginate/SAEE/HP-β-CD inclusion complex	1536:1581	In conclusion, coinciding with the experimental results, the molecular modeling successfully calculates the reasonable molecular structure and molecular behavior of sodium alginate/SAEE/HP-β-CD inclusion complex.
33415749	11	119	theme	complex	2021:2027	arg1	geometry					1999:2006	the geometry	1995:2006	the geometry of the final complex	1995:2027	The present study demonstrates a successful application of molecular modeling to predict the geometry of the final complex.
33415749	11	120	theme	modeling	1975:1982	arg1	application					1950:1960	a successful application	1937:1960	a successful application of molecular modeling to predict the geometry of the final complex	1937:2027	The present study demonstrates a successful application of molecular modeling to predict the geometry of the final complex.
34960836	4	0	theme	main	443:446	arg1	components					463:472	the main polysaccharide components	439:472	the main polysaccharide components	439:472	In this study, the main polysaccharide components were analyzed, in order to propose possible usage options.
34960836	5	1	gly	glycosylated	811:822	arg1	glycoproteins					824:836	highly glycosylated glycoproteins	804:836	highly glycosylated glycoproteins	804:836	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	1	2	theme	washed	129:134	arg1	amounts					91:97	high amounts	86:97	high amounts of dead seagrass material	86:123	Each year, high amounts of dead seagrass material are washed ashore at beaches world-wide.
34960836	1	2	theme	washed	129:134	arg1	material					116:123	dead seagrass material	102:123	dead seagrass material	102:123	Each year, high amounts of dead seagrass material are washed ashore at beaches world-wide.
34960836	1	2	theme	washed	129:134	arg1	ashore					136:141	washed ashore	129:141	washed ashore at beaches world-wide	129:163	Each year, high amounts of dead seagrass material are washed ashore at beaches world-wide.
34960836	7	3	theme	glycoproteins	1007:1019	arg1	glycoproteins					1007:1019	glycoproteins	1007:1019	glycoproteins	1007:1019	Only low amounts of glycoproteins, as well as medium amounts of the characteristic apiogalacturonan were likely to be present, while xylan seemed to be the most abundant polysaccharide in most fractions.
34960836	7	3	theme	glycoproteins	1007:1019	arg1	amounts					996:1002	Only low amounts	987:1002	Only low amounts of glycoproteins	987:1019	Only low amounts of glycoproteins, as well as medium amounts of the characteristic apiogalacturonan were likely to be present, while xylan seemed to be the most abundant polysaccharide in most fractions.
34960836	7	3	theme	glycoproteins	1007:1019	arg1	amounts					1040:1046	medium amounts	1033:1046	medium amounts of the characteristic apiogalacturonan	1033:1085	Only low amounts of glycoproteins, as well as medium amounts of the characteristic apiogalacturonan were likely to be present, while xylan seemed to be the most abundant polysaccharide in most fractions.
34960836	7	3	theme	glycoproteins	1007:1019	arg1	apiogalacturonan					1070:1085	the characteristic apiogalacturonan	1051:1085	the characteristic apiogalacturonan	1051:1085	Only low amounts of glycoproteins, as well as medium amounts of the characteristic apiogalacturonan were likely to be present, while xylan seemed to be the most abundant polysaccharide in most fractions.
34960836	5	4	theme	ion-exchange	713:724	arg1	chromatography					726:739	ion-exchange chromatography	713:739	ion-exchange chromatography	713:739	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	5	5	theme	carbohydrate	611:622	arg1	GC-FID/MS					642:650	GC-FID/MS	642:650	GC-FID/MS	642:650	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	5	5	theme	carbohydrate	611:622	arg1	assay					666:670	colorimetric assay	653:670	colorimetric assay	653:670	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	5	5	theme	carbohydrate	611:622	arg1	methods					633:639	classical carbohydrate analysis methods	601:639	classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis)	601:694	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	5	5	theme	carbohydrate	611:622	arg1	analysis					686:693	elemental analysis	676:693	elemental analysis	676:693	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	8	6	theme	generation	1335:1344	arg1	field					1303:1307	the field	1299:1307	the field of biofuel and bioplastic generation	1299:1344	A partial structural proposal showed general accordance with land plant xylans, presenting reuse options in the field of biofuel and bioplastic generation.
34960836	3	7	theme	touristic	328:336	arg1	reasons					338:344	touristic reasons	328:344	touristic reasons	328:344	As these are often removed due to touristic reasons, a reuse method would be a step towards a more ecologically oriented society.
34960836	4	8	dep	analyzed	479:486	arg1	propose					501:507	propose	501:507	to propose possible usage options	498:530	In this study, the main polysaccharide components were analyzed, in order to propose possible usage options.
34960836	5	9	theme	classical	601:609	arg1	GC-FID/MS					642:650	GC-FID/MS	642:650	GC-FID/MS	642:650	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	5	9	theme	classical	601:609	arg1	assay					666:670	colorimetric assay	653:670	colorimetric assay	653:670	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	5	9	theme	classical	601:609	arg1	methods					633:639	classical carbohydrate analysis methods	601:639	classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis)	601:694	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	5	9	theme	classical	601:609	arg1	analysis					686:693	elemental analysis	676:693	elemental analysis	676:693	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	2	10	theme	ball-like	267:275	arg1	litter					286:291	ball-like seagrass litter	267:291	ball-like seagrass litter	267:291	In the Mediterranean region, the seagrass Posidonia oceanica is responsible for huge agglomerates of ball-like seagrass litter.
34960836	2	11	theme	seagrass	277:284	arg1	litter					286:291	ball-like seagrass litter	267:291	ball-like seagrass litter	267:291	In the Mediterranean region, the seagrass Posidonia oceanica is responsible for huge agglomerates of ball-like seagrass litter.
34960836	8	12	theme	bioplastic	1324:1333	arg1	generation					1335:1344	bioplastic generation	1324:1344	bioplastic generation	1324:1344	A partial structural proposal showed general accordance with land plant xylans, presenting reuse options in the field of biofuel and bioplastic generation.
34960836	5	13	gly	glycoproteins	824:836	arg1	glycoproteins					824:836	highly glycosylated glycoproteins	804:836	highly glycosylated glycoproteins	804:836	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	5	14	theme	different	545:553	arg1	fractions					563:571	different aqueous fractions	545:571	different aqueous fractions	545:571	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	8	15	theme	land	1252:1255	arg1	xylans					1263:1268	land plant xylans	1252:1268	land plant xylans	1252:1268	A partial structural proposal showed general accordance with land plant xylans, presenting reuse options in the field of biofuel and bioplastic generation.
34960836	6	16	theme	extract	945:951	arg1	analysis					914:921	a linkage-type analysis	899:921	a linkage-type analysis of the most promising extract	899:951	The obtained purified fractions were analyzed in detail and a linkage-type analysis of the most promising extract was conducted via permethylation.
34960836	2	17	from	region	187:192	arg1	responsible					230:240	responsible	230:240	responsible	230:240	In the Mediterranean region, the seagrass Posidonia oceanica is responsible for huge agglomerates of ball-like seagrass litter.
34960836	2	17	from	region	187:192	arg1	oceanica					218:225	the seagrass Posidonia oceanica	195:225	the seagrass Posidonia oceanica	195:225	In the Mediterranean region, the seagrass Posidonia oceanica is responsible for huge agglomerates of ball-like seagrass litter.
34960836	6	18	theme	purified	852:859	arg1	fractions					861:869	The obtained purified fractions	839:869	The obtained purified fractions	839:869	The obtained purified fractions were analyzed in detail and a linkage-type analysis of the most promising extract was conducted via permethylation.
34960836	5	19	theme	aqueous	555:561	arg1	fractions					563:571	different aqueous fractions	545:571	different aqueous fractions	545:571	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	5	20	with	chromatography	726:739	arg1	agent					794:798	a detecting agent	782:798	a detecting agent for highly glycosylated glycoproteins	782:836	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	8	21	theme	partial	1193:1199	arg1	proposal					1212:1219	A partial structural proposal	1191:1219	A partial structural proposal	1191:1219	A partial structural proposal showed general accordance with land plant xylans, presenting reuse options in the field of biofuel and bioplastic generation.
34960836	5	22	dep	methods	633:639	arg1	GC-FID/MS					642:650	GC-FID/MS	642:650	GC-FID/MS	642:650	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	5	22	dep	methods	633:639	arg1	assay					666:670	colorimetric assay	653:670	colorimetric assay	653:670	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	5	22	dep	methods	633:639	arg1	analysis					686:693	elemental analysis	676:693	elemental analysis	676:693	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	5	22	dep	methods	633:639	arg1	methods					633:639	classical carbohydrate analysis methods	601:639	classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis)	601:694	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	6	23	theme	obtained	843:850	arg1	fractions					861:869	The obtained purified fractions	839:869	The obtained purified fractions	839:869	The obtained purified fractions were analyzed in detail and a linkage-type analysis of the most promising extract was conducted via permethylation.
34960836	7	24	theme	characteristic	1055:1068	arg1	apiogalacturonan					1070:1085	the characteristic apiogalacturonan	1051:1085	the characteristic apiogalacturonan	1051:1085	Only low amounts of glycoproteins, as well as medium amounts of the characteristic apiogalacturonan were likely to be present, while xylan seemed to be the most abundant polysaccharide in most fractions.
34960836	7	25	theme	medium	1033:1038	arg1	glycoproteins					1007:1019	glycoproteins	1007:1019	glycoproteins	1007:1019	Only low amounts of glycoproteins, as well as medium amounts of the characteristic apiogalacturonan were likely to be present, while xylan seemed to be the most abundant polysaccharide in most fractions.
34960836	7	25	theme	medium	1033:1038	arg1	amounts					1040:1046	medium amounts	1033:1046	medium amounts of the characteristic apiogalacturonan	1033:1085	Only low amounts of glycoproteins, as well as medium amounts of the characteristic apiogalacturonan were likely to be present, while xylan seemed to be the most abundant polysaccharide in most fractions.
34960836	7	25	theme	medium	1033:1038	arg1	apiogalacturonan					1070:1085	the characteristic apiogalacturonan	1051:1085	the characteristic apiogalacturonan	1051:1085	Only low amounts of glycoproteins, as well as medium amounts of the characteristic apiogalacturonan were likely to be present, while xylan seemed to be the most abundant polysaccharide in most fractions.
34960836	2	26	theme	huge	246:249	arg1	agglomerates					251:262	huge agglomerates	246:262	huge agglomerates of ball-like seagrass litter	246:291	In the Mediterranean region, the seagrass Posidonia oceanica is responsible for huge agglomerates of ball-like seagrass litter.
34960836	1	27	theme	beaches	146:152	arg1	world-wide					154:163	beaches world-wide	146:163	beaches world-wide	146:163	Each year, high amounts of dead seagrass material are washed ashore at beaches world-wide.
34960836	0	28	theme	Neptune	1:7	arg1	Polysaccharides					16:30	"Neptune Balls" Polysaccharides	0:30	"Neptune Balls" Polysaccharides: Disentangling the Wiry Seagrass Detritus.	0:73	"Neptune Balls" Polysaccharides: Disentangling the Wiry Seagrass Detritus.
34960836	7	29	theme	most	1175:1178	arg1	fractions					1180:1188	most fractions	1175:1188	most fractions	1175:1188	Only low amounts of glycoproteins, as well as medium amounts of the characteristic apiogalacturonan were likely to be present, while xylan seemed to be the most abundant polysaccharide in most fractions.
34960836	5	30	with	precipitation	763:775	arg1	agent					794:798	a detecting agent	782:798	a detecting agent for highly glycosylated glycoproteins	782:836	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	8	31	with	accordance	1236:1245	arg1	xylans					1263:1268	land plant xylans	1252:1268	land plant xylans	1252:1268	A partial structural proposal showed general accordance with land plant xylans, presenting reuse options in the field of biofuel and bioplastic generation.
34960836	5	32	theme	selective	753:761	arg1	precipitation					763:775	selective precipitation	753:775	selective precipitation with a detecting agent for highly glycosylated glycoproteins	753:836	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	6	33	theme	promising	935:943	arg1	extract					945:951	the most promising extract	926:951	the most promising extract	926:951	The obtained purified fractions were analyzed in detail and a linkage-type analysis of the most promising extract was conducted via permethylation.
34960836	8	34	theme	biofuel	1312:1318	arg1	field					1303:1307	the field	1299:1307	the field of biofuel and bioplastic generation	1299:1344	A partial structural proposal showed general accordance with land plant xylans, presenting reuse options in the field of biofuel and bioplastic generation.
34960836	8	35	theme	structural	1201:1210	arg1	proposal					1212:1219	A partial structural proposal	1191:1219	A partial structural proposal	1191:1219	A partial structural proposal showed general accordance with land plant xylans, presenting reuse options in the field of biofuel and bioplastic generation.
34960836	0	36	theme	"	14:14	arg1	Polysaccharides					16:30	"Neptune Balls" Polysaccharides	0:30	"Neptune Balls" Polysaccharides: Disentangling the Wiry Seagrass Detritus.	0:73	"Neptune Balls" Polysaccharides: Disentangling the Wiry Seagrass Detritus.
34960836	2	37	theme	litter	286:291	arg1	agglomerates					251:262	huge agglomerates	246:262	huge agglomerates of ball-like seagrass litter	246:291	In the Mediterranean region, the seagrass Posidonia oceanica is responsible for huge agglomerates of ball-like seagrass litter.
34960836	8	38	theme	general	1228:1234	arg1	accordance					1236:1245	general accordance	1228:1245	general accordance with land plant xylans	1228:1268	A partial structural proposal showed general accordance with land plant xylans, presenting reuse options in the field of biofuel and bioplastic generation.
34960836	0	39	theme	Balls	9:13	arg1	Polysaccharides					16:30	"Neptune Balls" Polysaccharides	0:30	"Neptune Balls" Polysaccharides: Disentangling the Wiry Seagrass Detritus.	0:73	"Neptune Balls" Polysaccharides: Disentangling the Wiry Seagrass Detritus.
34960836	4	40	theme	usage	518:522	arg1	options					524:530	possible usage options	509:530	possible usage options	509:530	In this study, the main polysaccharide components were analyzed, in order to propose possible usage options.
34960836	5	41	theme	colorimetric	653:664	arg1	assay					666:670	colorimetric assay	653:670	colorimetric assay	653:670	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	5	41	theme	colorimetric	653:664	arg1	methods					633:639	classical carbohydrate analysis methods	601:639	classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis)	601:694	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	7	42	theme	apiogalacturonan	1070:1085	arg1	glycoproteins					1007:1019	glycoproteins	1007:1019	glycoproteins	1007:1019	Only low amounts of glycoproteins, as well as medium amounts of the characteristic apiogalacturonan were likely to be present, while xylan seemed to be the most abundant polysaccharide in most fractions.
34960836	7	42	theme	apiogalacturonan	1070:1085	arg1	amounts					996:1002	Only low amounts	987:1002	Only low amounts of glycoproteins	987:1019	Only low amounts of glycoproteins, as well as medium amounts of the characteristic apiogalacturonan were likely to be present, while xylan seemed to be the most abundant polysaccharide in most fractions.
34960836	7	42	theme	apiogalacturonan	1070:1085	arg1	amounts					1040:1046	medium amounts	1033:1046	medium amounts of the characteristic apiogalacturonan	1033:1085	Only low amounts of glycoproteins, as well as medium amounts of the characteristic apiogalacturonan were likely to be present, while xylan seemed to be the most abundant polysaccharide in most fractions.
34960836	7	42	theme	apiogalacturonan	1070:1085	arg1	apiogalacturonan					1070:1085	the characteristic apiogalacturonan	1051:1085	the characteristic apiogalacturonan	1051:1085	Only low amounts of glycoproteins, as well as medium amounts of the characteristic apiogalacturonan were likely to be present, while xylan seemed to be the most abundant polysaccharide in most fractions.
34960836	1	43	theme	high	86:89	arg1	amounts					91:97	high amounts	86:97	high amounts of dead seagrass material	86:123	Each year, high amounts of dead seagrass material are washed ashore at beaches world-wide.
34960836	1	43	theme	high	86:89	arg1	material					116:123	dead seagrass material	102:123	dead seagrass material	102:123	Each year, high amounts of dead seagrass material are washed ashore at beaches world-wide.
34960836	1	43	theme	high	86:89	arg1	ashore					136:141	washed ashore	129:141	washed ashore at beaches world-wide	129:163	Each year, high amounts of dead seagrass material are washed ashore at beaches world-wide.
34960836	5	44	theme	detecting	784:792	arg1	agent					794:798	a detecting agent	782:798	a detecting agent for highly glycosylated glycoproteins	782:836	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	7	45	theme	abundant	1148:1155	arg1	polysaccharide					1157:1170	the most abundant polysaccharide	1139:1170	the most abundant polysaccharide in most fractions	1139:1188	Only low amounts of glycoproteins, as well as medium amounts of the characteristic apiogalacturonan were likely to be present, while xylan seemed to be the most abundant polysaccharide in most fractions.
34960836	7	45	theme	abundant	1148:1155	arg1	xylan					1120:1124	xylan	1120:1124	xylan	1120:1124	Only low amounts of glycoproteins, as well as medium amounts of the characteristic apiogalacturonan were likely to be present, while xylan seemed to be the most abundant polysaccharide in most fractions.
34960836	4	46	theme	possible	509:516	arg1	options					524:530	possible usage options	509:530	possible usage options	509:530	In this study, the main polysaccharide components were analyzed, in order to propose possible usage options.
34960836	2	47	theme	Posidonia	208:216	arg1	responsible					230:240	responsible	230:240	responsible	230:240	In the Mediterranean region, the seagrass Posidonia oceanica is responsible for huge agglomerates of ball-like seagrass litter.
34960836	2	47	theme	Posidonia	208:216	arg1	oceanica					218:225	the seagrass Posidonia oceanica	195:225	the seagrass Posidonia oceanica	195:225	In the Mediterranean region, the seagrass Posidonia oceanica is responsible for huge agglomerates of ball-like seagrass litter.
34960836	2	48	from	responsible	230:240	arg1	region					187:192	the Mediterranean region	169:192	the Mediterranean region	169:192	In the Mediterranean region, the seagrass Posidonia oceanica is responsible for huge agglomerates of ball-like seagrass litter.
34960836	2	49	theme	seagrass	199:206	arg1	responsible					230:240	responsible	230:240	responsible	230:240	In the Mediterranean region, the seagrass Posidonia oceanica is responsible for huge agglomerates of ball-like seagrass litter.
34960836	2	49	theme	seagrass	199:206	arg1	oceanica					218:225	the seagrass Posidonia oceanica	195:225	the seagrass Posidonia oceanica	195:225	In the Mediterranean region, the seagrass Posidonia oceanica is responsible for huge agglomerates of ball-like seagrass litter.
34960836	5	50	theme	elemental	676:684	arg1	analysis					686:693	elemental analysis	676:693	elemental analysis	676:693	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	5	50	theme	elemental	676:684	arg1	methods					633:639	classical carbohydrate analysis methods	601:639	classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis)	601:694	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	5	51	theme	analysis	624:631	arg1	GC-FID/MS					642:650	GC-FID/MS	642:650	GC-FID/MS	642:650	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	5	51	theme	analysis	624:631	arg1	assay					666:670	colorimetric assay	653:670	colorimetric assay	653:670	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	5	51	theme	analysis	624:631	arg1	methods					633:639	classical carbohydrate analysis methods	601:639	classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis)	601:694	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	5	51	theme	analysis	624:631	arg1	analysis					686:693	elemental analysis	676:693	elemental analysis	676:693	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	7	52	from	polysaccharide	1157:1170	arg1	fractions					1180:1188	most fractions	1175:1188	most fractions	1175:1188	Only low amounts of glycoproteins, as well as medium amounts of the characteristic apiogalacturonan were likely to be present, while xylan seemed to be the most abundant polysaccharide in most fractions.
34960836	7	53	theme	low	992:994	arg1	glycoproteins					1007:1019	glycoproteins	1007:1019	glycoproteins	1007:1019	Only low amounts of glycoproteins, as well as medium amounts of the characteristic apiogalacturonan were likely to be present, while xylan seemed to be the most abundant polysaccharide in most fractions.
34960836	7	53	theme	low	992:994	arg1	amounts					996:1002	Only low amounts	987:1002	Only low amounts of glycoproteins	987:1019	Only low amounts of glycoproteins, as well as medium amounts of the characteristic apiogalacturonan were likely to be present, while xylan seemed to be the most abundant polysaccharide in most fractions.
34960836	7	53	theme	low	992:994	arg1	apiogalacturonan					1070:1085	the characteristic apiogalacturonan	1051:1085	the characteristic apiogalacturonan	1051:1085	Only low amounts of glycoproteins, as well as medium amounts of the characteristic apiogalacturonan were likely to be present, while xylan seemed to be the most abundant polysaccharide in most fractions.
34960836	8	54	theme	plant	1257:1261	arg1	xylans					1263:1268	land plant xylans	1252:1268	land plant xylans	1252:1268	A partial structural proposal showed general accordance with land plant xylans, presenting reuse options in the field of biofuel and bioplastic generation.
34960836	0	55	dep	Polysaccharides	16:30	arg1	Disentangling					33:45	Disentangling	33:45	Disentangling the Wiry Seagrass Detritus	33:72	"Neptune Balls" Polysaccharides: Disentangling the Wiry Seagrass Detritus.
34960836	7	56	gly	glycoproteins	1007:1019	arg1	glycoproteins					1007:1019	glycoproteins	1007:1019	glycoproteins	1007:1019	Only low amounts of glycoproteins, as well as medium amounts of the characteristic apiogalacturonan were likely to be present, while xylan seemed to be the most abundant polysaccharide in most fractions.
34960836	2	57	theme	Mediterranean	173:185	arg1	region					187:192	the Mediterranean region	169:192	the Mediterranean region	169:192	In the Mediterranean region, the seagrass Posidonia oceanica is responsible for huge agglomerates of ball-like seagrass litter.
34960836	1	58	theme	dead	102:105	arg1	material					116:123	dead seagrass material	102:123	dead seagrass material	102:123	Each year, high amounts of dead seagrass material are washed ashore at beaches world-wide.
34960836	8	59	theme	reuse	1282:1286	arg1	options					1288:1294	reuse options	1282:1294	reuse options	1282:1294	A partial structural proposal showed general accordance with land plant xylans, presenting reuse options in the field of biofuel and bioplastic generation.
34960836	1	60	from	world-wide	154:163	arg1	amounts					91:97	high amounts	86:97	high amounts of dead seagrass material	86:123	Each year, high amounts of dead seagrass material are washed ashore at beaches world-wide.
34960836	1	60	from	world-wide	154:163	arg1	material					116:123	dead seagrass material	102:123	dead seagrass material	102:123	Each year, high amounts of dead seagrass material are washed ashore at beaches world-wide.
34960836	1	60	from	world-wide	154:163	arg1	ashore					136:141	washed ashore	129:141	washed ashore at beaches world-wide	129:163	Each year, high amounts of dead seagrass material are washed ashore at beaches world-wide.
34960836	1	61	theme	seagrass	107:114	arg1	material					116:123	dead seagrass material	102:123	dead seagrass material	102:123	Each year, high amounts of dead seagrass material are washed ashore at beaches world-wide.
34960836	0	62	theme	Seagrass	56:63	arg1	Detritus					65:72	the Wiry Seagrass Detritus	47:72	the Wiry Seagrass Detritus	47:72	"Neptune Balls" Polysaccharides: Disentangling the Wiry Seagrass Detritus.
34960836	1	63	theme	material	116:123	arg1	amounts					91:97	high amounts	86:97	high amounts of dead seagrass material	86:123	Each year, high amounts of dead seagrass material are washed ashore at beaches world-wide.
34960836	1	63	theme	material	116:123	arg1	material					116:123	dead seagrass material	102:123	dead seagrass material	102:123	Each year, high amounts of dead seagrass material are washed ashore at beaches world-wide.
34960836	1	63	theme	material	116:123	arg1	ashore					136:141	washed ashore	129:141	washed ashore at beaches world-wide	129:163	Each year, high amounts of dead seagrass material are washed ashore at beaches world-wide.
34960836	5	64	theme	glycosylated	811:822	arg1	glycoproteins					824:836	highly glycosylated glycoproteins	804:836	highly glycosylated glycoproteins	804:836	To do this, different aqueous fractions were extracted, analyzed by classical carbohydrate analysis methods (GC-FID/MS, colorimetric assay and elemental analysis), and purified by ion-exchange chromatography, as well as selective precipitation with a detecting agent for highly glycosylated glycoproteins.
34960836	0	65	theme	Wiry	51:54	arg1	Detritus					65:72	the Wiry Seagrass Detritus	47:72	the Wiry Seagrass Detritus	47:72	"Neptune Balls" Polysaccharides: Disentangling the Wiry Seagrass Detritus.
34960836	4	66	theme	polysaccharide	448:461	arg1	components					463:472	the main polysaccharide components	439:472	the main polysaccharide components	439:472	In this study, the main polysaccharide components were analyzed, in order to propose possible usage options.
34960836	3	67	theme	reuse	349:353	arg1	method					355:360	a reuse method	347:360	a reuse method	347:360	As these are often removed due to touristic reasons, a reuse method would be a step towards a more ecologically oriented society.
34960836	3	67	theme	reuse	349:353	arg1	step					373:376	a step	371:376	a step towards a more ecologically oriented society	371:421	As these are often removed due to touristic reasons, a reuse method would be a step towards a more ecologically oriented society.
34960836	6	68	theme	linkage-type	901:912	arg1	analysis					914:921	a linkage-type analysis	899:921	a linkage-type analysis of the most promising extract	899:951	The obtained purified fractions were analyzed in detail and a linkage-type analysis of the most promising extract was conducted via permethylation.
34347454	0	0	theme	Polyelectrolyte	98:112	arg1	Surfaces					125:132	Polyelectrolyte Multilayer Surfaces	98:132	Polyelectrolyte Multilayer Surfaces	98:132	Enzymatic Degradation of Glycosaminoglycans and Proteoglycan-Mimetic Materials in Solution and on Polyelectrolyte Multilayer Surfaces.
34347454	7	1	theme	multilayer	1172:1181	arg1	surfaces					1183:1190	polyelectrolyte multilayer surfaces	1156:1190	polyelectrolyte multilayer surfaces	1156:1190	Adsorption to polyelectrolyte multilayer surfaces protects PG mimics from degradation, compared to when PG mimics are combined with enzymes in solution; all surfaces are still intact after 21 days of enzyme exposure.
34347454	2	2	dep	types	468:472	arg1	GCs					566:568	GCs	566:568	GCs	566:568	Our lab has designed two different types of PG mimics: polyelectrolyte complex nanoparticles (PCNs) and PG-mimetic graft copolymers (GCs), both of which are prepared using naturally occurring glycosaminoglycans.
34347454	2	2	dep	types	468:472	arg1	copolymers					554:563	PG-mimetic graft copolymers	537:563	PG-mimetic graft copolymers	537:563	Our lab has designed two different types of PG mimics: polyelectrolyte complex nanoparticles (PCNs) and PG-mimetic graft copolymers (GCs), both of which are prepared using naturally occurring glycosaminoglycans.
34347454	2	2	dep	types	468:472	arg1	PCNs					527:530	PCNs	527:530	PCNs	527:530	Our lab has designed two different types of PG mimics: polyelectrolyte complex nanoparticles (PCNs) and PG-mimetic graft copolymers (GCs), both of which are prepared using naturally occurring glycosaminoglycans.
34347454	2	2	dep	types	468:472	arg1	nanoparticles					512:524	polyelectrolyte complex nanoparticles	488:524	polyelectrolyte complex nanoparticles (PCNs)	488:531	Our lab has designed two different types of PG mimics: polyelectrolyte complex nanoparticles (PCNs) and PG-mimetic graft copolymers (GCs), both of which are prepared using naturally occurring glycosaminoglycans.
34347454	2	3	theme	complex	504:510	arg1	GCs					566:568	GCs	566:568	GCs	566:568	Our lab has designed two different types of PG mimics: polyelectrolyte complex nanoparticles (PCNs) and PG-mimetic graft copolymers (GCs), both of which are prepared using naturally occurring glycosaminoglycans.
34347454	2	3	theme	complex	504:510	arg1	PCNs					527:530	PCNs	527:530	PCNs	527:530	Our lab has designed two different types of PG mimics: polyelectrolyte complex nanoparticles (PCNs) and PG-mimetic graft copolymers (GCs), both of which are prepared using naturally occurring glycosaminoglycans.
34347454	2	3	theme	complex	504:510	arg1	nanoparticles					512:524	polyelectrolyte complex nanoparticles	488:524	polyelectrolyte complex nanoparticles (PCNs)	488:531	Our lab has designed two different types of PG mimics: polyelectrolyte complex nanoparticles (PCNs) and PG-mimetic graft copolymers (GCs), both of which are prepared using naturally occurring glycosaminoglycans.
34347454	0	4	from	Degradation	10:20	arg1	Surfaces					125:132	Polyelectrolyte Multilayer Surfaces	98:132	Polyelectrolyte Multilayer Surfaces	98:132	Enzymatic Degradation of Glycosaminoglycans and Proteoglycan-Mimetic Materials in Solution and on Polyelectrolyte Multilayer Surfaces.
34347454	0	4	from	Degradation	10:20	arg1	Solution					82:89	Solution	82:89	Solution	82:89	Enzymatic Degradation of Glycosaminoglycans and Proteoglycan-Mimetic Materials in Solution and on Polyelectrolyte Multilayer Surfaces.
34347454	2	5	dep	PG	477:478	arg1	mimics					480:485	mimics	480:485	mimics	480:485	Our lab has designed two different types of PG mimics: polyelectrolyte complex nanoparticles (PCNs) and PG-mimetic graft copolymers (GCs), both of which are prepared using naturally occurring glycosaminoglycans.
34347454	9	6	theme	factor	1711:1716	arg1	applications					1727:1738	growth factor delivery applications	1704:1738	growth factor delivery applications	1704:1738	Understanding and tuning these degradation susceptibilities are essential for advancing their applications in cardiovascular materials, orthopedic materials, and growth factor delivery applications.
34347454	1	7	theme	extracellular	267:279	arg1	components					288:297	extracellular matrix components	267:297	extracellular matrix components	267:297	Proteoglycans (PGs) play many important roles in biology, contributing to the mechanical properties of tissues, helping to organize extracellular matrix components, and participating in signaling mechanisms related to mechanotransduction, cell differentiation, immune responses, and wound healing.
34347454	2	8	theme	graft	548:552	arg1	copolymers					554:563	PG-mimetic graft copolymers	537:563	PG-mimetic graft copolymers	537:563	Our lab has designed two different types of PG mimics: polyelectrolyte complex nanoparticles (PCNs) and PG-mimetic graft copolymers (GCs), both of which are prepared using naturally occurring glycosaminoglycans.
34347454	8	9	dep	PG	1398:1399	arg1	mimics					1401:1406	mimics	1401:1406	mimics	1401:1406	This work reveals how the stability of PG mimics is controlled by both the composition and macromolecular assembly of the PG mimic and also by the size and specificity of the enzyme.
34347454	1	10	theme	matrix	281:286	arg1	components					288:297	extracellular matrix components	267:297	extracellular matrix components	267:297	Proteoglycans (PGs) play many important roles in biology, contributing to the mechanical properties of tissues, helping to organize extracellular matrix components, and participating in signaling mechanisms related to mechanotransduction, cell differentiation, immune responses, and wound healing.
34347454	0	11	theme	Multilayer	114:123	arg1	Surfaces					125:132	Polyelectrolyte Multilayer Surfaces	98:132	Polyelectrolyte Multilayer Surfaces	98:132	Enzymatic Degradation of Glycosaminoglycans and Proteoglycan-Mimetic Materials in Solution and on Polyelectrolyte Multilayer Surfaces.
34347454	3	12	theme	mimics	701:706	arg1	stability					679:687	the enzymatic stability	665:687	the enzymatic stability of these PG mimics using hyaluronidases (I-S, IV-S, and II), chondroitinase ABC, and lysozyme	665:781	This work evaluates the enzymatic stability of these PG mimics using hyaluronidases (I-S, IV-S, and II), chondroitinase ABC, and lysozyme, for PG mimics suspended in solution and adsorbed onto surfaces.
34347454	9	13	theme	cardiovascular	1652:1665	arg1	materials					1667:1675	cardiovascular materials	1652:1675	cardiovascular materials	1652:1675	Understanding and tuning these degradation susceptibilities are essential for advancing their applications in cardiovascular materials, orthopedic materials, and growth factor delivery applications.
34347454	9	14	from	applications	1727:1738	arg1	materials					1667:1675	cardiovascular materials	1652:1675	cardiovascular materials	1652:1675	Understanding and tuning these degradation susceptibilities are essential for advancing their applications in cardiovascular materials, orthopedic materials, and growth factor delivery applications.
34347454	1	15	theme	immune	396:401	arg1	responses					403:411	immune responses	396:411	immune responses	396:411	Proteoglycans (PGs) play many important roles in biology, contributing to the mechanical properties of tissues, helping to organize extracellular matrix components, and participating in signaling mechanisms related to mechanotransduction, cell differentiation, immune responses, and wound healing.
34347454	8	16	dep	size	1506:1509	arg1	the					1502:1504	the	1502:1504	the	1502:1504	This work reveals how the stability of PG mimics is controlled by both the composition and macromolecular assembly of the PG mimic and also by the size and specificity of the enzyme.
34347454	0	17	theme	Materials	69:77	arg1	Degradation					10:20	Enzymatic Degradation	0:20	Enzymatic Degradation of Glycosaminoglycans and Proteoglycan-Mimetic Materials in Solution and on Polyelectrolyte Multilayer Surfaces.	0:133	Enzymatic Degradation of Glycosaminoglycans and Proteoglycan-Mimetic Materials in Solution and on Polyelectrolyte Multilayer Surfaces.
34347454	2	18	theme	polyelectrolyte	488:502	arg1	GCs					566:568	GCs	566:568	GCs	566:568	Our lab has designed two different types of PG mimics: polyelectrolyte complex nanoparticles (PCNs) and PG-mimetic graft copolymers (GCs), both of which are prepared using naturally occurring glycosaminoglycans.
34347454	2	18	theme	polyelectrolyte	488:502	arg1	PCNs					527:530	PCNs	527:530	PCNs	527:530	Our lab has designed two different types of PG mimics: polyelectrolyte complex nanoparticles (PCNs) and PG-mimetic graft copolymers (GCs), both of which are prepared using naturally occurring glycosaminoglycans.
34347454	2	18	theme	polyelectrolyte	488:502	arg1	nanoparticles					512:524	polyelectrolyte complex nanoparticles	488:524	polyelectrolyte complex nanoparticles (PCNs)	488:531	Our lab has designed two different types of PG mimics: polyelectrolyte complex nanoparticles (PCNs) and PG-mimetic graft copolymers (GCs), both of which are prepared using naturally occurring glycosaminoglycans.
34347454	0	19	theme	Enzymatic	0:8	arg1	Degradation					10:20	Enzymatic Degradation	0:20	Enzymatic Degradation of Glycosaminoglycans and Proteoglycan-Mimetic Materials in Solution and on Polyelectrolyte Multilayer Surfaces.	0:133	Enzymatic Degradation of Glycosaminoglycans and Proteoglycan-Mimetic Materials in Solution and on Polyelectrolyte Multilayer Surfaces.
34347454	3	20	theme	chondroitinase	750:763	arg1	ABC					765:767	chondroitinase ABC	750:767	chondroitinase ABC	750:767	This work evaluates the enzymatic stability of these PG mimics using hyaluronidases (I-S, IV-S, and II), chondroitinase ABC, and lysozyme, for PG mimics suspended in solution and adsorbed onto surfaces.
34347454	9	21	theme	delivery	1718:1725	arg1	applications					1727:1738	growth factor delivery applications	1704:1738	growth factor delivery applications	1704:1738	Understanding and tuning these degradation susceptibilities are essential for advancing their applications in cardiovascular materials, orthopedic materials, and growth factor delivery applications.
34347454	9	22	theme	degradation	1573:1583	arg1	susceptibilities					1585:1600	these degradation susceptibilities	1567:1600	these degradation susceptibilities	1567:1600	Understanding and tuning these degradation susceptibilities are essential for advancing their applications in cardiovascular materials, orthopedic materials, and growth factor delivery applications.
34347454	7	23	theme	exposure	1349:1356	arg1	days					1334:1337	21 days	1331:1337	21 days of enzyme exposure	1331:1356	Adsorption to polyelectrolyte multilayer surfaces protects PG mimics from degradation, compared to when PG mimics are combined with enzymes in solution; all surfaces are still intact after 21 days of enzyme exposure.
34347454	8	24	theme	macromolecular	1450:1463	arg1	assembly					1465:1472	macromolecular assembly	1450:1472	macromolecular assembly	1450:1472	This work reveals how the stability of PG mimics is controlled by both the composition and macromolecular assembly of the PG mimic and also by the size and specificity of the enzyme.
34347454	1	25	theme	cell	374:377	arg1	differentiation					379:393	cell differentiation	374:393	cell differentiation	374:393	Proteoglycans (PGs) play many important roles in biology, contributing to the mechanical properties of tissues, helping to organize extracellular matrix components, and participating in signaling mechanisms related to mechanotransduction, cell differentiation, immune responses, and wound healing.
34347454	2	26	theme	occurring	615:623	arg1	glycosaminoglycans					625:642	naturally occurring glycosaminoglycans	605:642	naturally occurring glycosaminoglycans	605:642	Our lab has designed two different types of PG mimics: polyelectrolyte complex nanoparticles (PCNs) and PG-mimetic graft copolymers (GCs), both of which are prepared using naturally occurring glycosaminoglycans.
34347454	6	27	theme	materials	1095:1103	arg1	None					1083:1086	None	1083:1086	None of the materials	1083:1103	None of the materials are measurably degraded by lysozyme.
34347454	3	28	theme	enzymatic	669:677	arg1	stability					679:687	the enzymatic stability	665:687	the enzymatic stability of these PG mimics using hyaluronidases (I-S, IV-S, and II), chondroitinase ABC, and lysozyme	665:781	This work evaluates the enzymatic stability of these PG mimics using hyaluronidases (I-S, IV-S, and II), chondroitinase ABC, and lysozyme, for PG mimics suspended in solution and adsorbed onto surfaces.
34347454	0	29	theme	Glycosaminoglycans	25:42	arg1	Materials					69:77	Glycosaminoglycans and Proteoglycan-Mimetic Materials	25:77	Glycosaminoglycans and Proteoglycan-Mimetic Materials	25:77	Enzymatic Degradation of Glycosaminoglycans and Proteoglycan-Mimetic Materials in Solution and on Polyelectrolyte Multilayer Surfaces.
34347454	8	30	theme	PG	1481:1482	arg1	composition					1434:1444	composition	1434:1444	composition	1434:1444	This work reveals how the stability of PG mimics is controlled by both the composition and macromolecular assembly of the PG mimic and also by the size and specificity of the enzyme.
34347454	3	31	dep	hyaluronidases	714:727	arg1	IV-S					735:738	IV-S	735:738	IV-S	735:738	This work evaluates the enzymatic stability of these PG mimics using hyaluronidases (I-S, IV-S, and II), chondroitinase ABC, and lysozyme, for PG mimics suspended in solution and adsorbed onto surfaces.
34347454	3	31	dep	hyaluronidases	714:727	arg1	II					745:746	II	745:746	II	745:746	This work evaluates the enzymatic stability of these PG mimics using hyaluronidases (I-S, IV-S, and II), chondroitinase ABC, and lysozyme, for PG mimics suspended in solution and adsorbed onto surfaces.
34347454	3	31	dep	hyaluronidases	714:727	arg1	I-S					730:732	I-S	730:732	I-S	730:732	This work evaluates the enzymatic stability of these PG mimics using hyaluronidases (I-S, IV-S, and II), chondroitinase ABC, and lysozyme, for PG mimics suspended in solution and adsorbed onto surfaces.
34347454	7	32	from	enzymes	1274:1280	arg1	solution					1285:1292	solution	1285:1292	solution	1285:1292	Adsorption to polyelectrolyte multilayer surfaces protects PG mimics from degradation, compared to when PG mimics are combined with enzymes in solution; all surfaces are still intact after 21 days of enzyme exposure.
34347454	7	33	theme	enzyme	1342:1347	arg1	exposure					1349:1356	enzyme exposure	1342:1356	enzyme exposure	1342:1356	Adsorption to polyelectrolyte multilayer surfaces protects PG mimics from degradation, compared to when PG mimics are combined with enzymes in solution; all surfaces are still intact after 21 days of enzyme exposure.
34347454	1	34	theme	mechanical	213:222	arg1	properties					224:233	the mechanical properties	209:233	the mechanical properties of tissues	209:244	Proteoglycans (PGs) play many important roles in biology, contributing to the mechanical properties of tissues, helping to organize extracellular matrix components, and participating in signaling mechanisms related to mechanotransduction, cell differentiation, immune responses, and wound healing.
34347454	0	35	theme	Proteoglycan-Mimetic	48:67	arg1	Materials					69:77	Glycosaminoglycans and Proteoglycan-Mimetic Materials	25:77	Glycosaminoglycans and Proteoglycan-Mimetic Materials	25:77	Enzymatic Degradation of Glycosaminoglycans and Proteoglycan-Mimetic Materials in Solution and on Polyelectrolyte Multilayer Surfaces.
34347454	4	36	theme	-containing	893:903	arg1	mimics					908:913	Hyaluronan (HA)- and chondroitin sulfate (CS)-containing PG mimics	848:913	Hyaluronan (HA)- and chondroitin sulfate (CS)-containing PG mimics	848:913	Hyaluronan (HA)- and chondroitin sulfate (CS)-containing PG mimics are degraded by the hyaluronidases.
34347454	1	37	theme	wound	418:422	arg1	healing					424:430	wound healing	418:430	wound healing	418:430	Proteoglycans (PGs) play many important roles in biology, contributing to the mechanical properties of tissues, helping to organize extracellular matrix components, and participating in signaling mechanisms related to mechanotransduction, cell differentiation, immune responses, and wound healing.
34347454	1	38	theme	signaling	321:329	arg1	mechanisms					331:340	signaling mechanisms	321:340	signaling mechanisms related to mechanotransduction, cell differentiation, immune responses, and wound healing	321:430	Proteoglycans (PGs) play many important roles in biology, contributing to the mechanical properties of tissues, helping to organize extracellular matrix components, and participating in signaling mechanisms related to mechanotransduction, cell differentiation, immune responses, and wound healing.
34347454	8	39	dep	composition	1434:1444	arg1	the					1430:1432	the	1430:1432	the	1430:1432	This work reveals how the stability of PG mimics is controlled by both the composition and macromolecular assembly of the PG mimic and also by the size and specificity of the enzyme.
34347454	8	40	theme	PG	1398:1399	arg1	stability					1385:1393	the stability	1381:1393	the stability of PG mimics	1381:1406	This work reveals how the stability of PG mimics is controlled by both the composition and macromolecular assembly of the PG mimic and also by the size and specificity of the enzyme.
34347454	2	41	theme	different	458:466	arg1	types					468:472	two different types	454:472	two different types of PG mimics: polyelectrolyte complex nanoparticles (PCNs) and PG-mimetic graft copolymers (GCs), both of which are prepared using naturally occurring glycosaminoglycans	454:642	Our lab has designed two different types of PG mimics: polyelectrolyte complex nanoparticles (PCNs) and PG-mimetic graft copolymers (GCs), both of which are prepared using naturally occurring glycosaminoglycans.
34347454	9	42	dep	Understanding	1542:1554	arg1	susceptibilities					1585:1600	these degradation susceptibilities	1567:1600	these degradation susceptibilities	1567:1600	Understanding and tuning these degradation susceptibilities are essential for advancing their applications in cardiovascular materials, orthopedic materials, and growth factor delivery applications.
34347454	4	43	theme	PG	905:906	arg1	mimics					908:913	Hyaluronan (HA)- and chondroitin sulfate (CS)-containing PG mimics	848:913	Hyaluronan (HA)- and chondroitin sulfate (CS)-containing PG mimics	848:913	Hyaluronan (HA)- and chondroitin sulfate (CS)-containing PG mimics are degraded by the hyaluronidases.
34347454	5	44	theme	PG	1038:1039	arg1	GCs					977:979	GCs	977:979	GCs	977:979	PCNs prepared with CS and GCs prepared with heparin are the only CS- and HA-containing PG mimics protected from chondroitinase ABC.
34347454	5	44	theme	PG	1038:1039	arg1	mimics					1041:1046	the only CS- and HA-containing PG mimics	1007:1046	the only CS- and HA-containing PG mimics protected from chondroitinase ABC	1007:1080	PCNs prepared with CS and GCs prepared with heparin are the only CS- and HA-containing PG mimics protected from chondroitinase ABC.
34347454	5	44	theme	PG	1038:1039	arg1	PCNs					951:954	PCNs	951:954	PCNs prepared with CS	951:971	PCNs prepared with CS and GCs prepared with heparin are the only CS- and HA-containing PG mimics protected from chondroitinase ABC.
34347454	5	45	theme	HA-containing	1024:1036	arg1	GCs					977:979	GCs	977:979	GCs	977:979	PCNs prepared with CS and GCs prepared with heparin are the only CS- and HA-containing PG mimics protected from chondroitinase ABC.
34347454	5	45	theme	HA-containing	1024:1036	arg1	mimics					1041:1046	the only CS- and HA-containing PG mimics	1007:1046	the only CS- and HA-containing PG mimics protected from chondroitinase ABC	1007:1080	PCNs prepared with CS and GCs prepared with heparin are the only CS- and HA-containing PG mimics protected from chondroitinase ABC.
34347454	5	45	theme	HA-containing	1024:1036	arg1	PCNs					951:954	PCNs	951:954	PCNs prepared with CS	951:971	PCNs prepared with CS and GCs prepared with heparin are the only CS- and HA-containing PG mimics protected from chondroitinase ABC.
34347454	2	46	theme	PG	477:478	arg1	types					468:472	two different types	454:472	two different types of PG mimics: polyelectrolyte complex nanoparticles (PCNs) and PG-mimetic graft copolymers (GCs), both of which are prepared using naturally occurring glycosaminoglycans	454:642	Our lab has designed two different types of PG mimics: polyelectrolyte complex nanoparticles (PCNs) and PG-mimetic graft copolymers (GCs), both of which are prepared using naturally occurring glycosaminoglycans.
34347454	1	47	theme	related	342:348	arg1	mechanisms					331:340	signaling mechanisms	321:340	signaling mechanisms related to mechanotransduction, cell differentiation, immune responses, and wound healing	321:430	Proteoglycans (PGs) play many important roles in biology, contributing to the mechanical properties of tissues, helping to organize extracellular matrix components, and participating in signaling mechanisms related to mechanotransduction, cell differentiation, immune responses, and wound healing.
34347454	9	48	theme	orthopedic	1678:1687	arg1	materials					1689:1697	orthopedic materials	1678:1697	orthopedic materials	1678:1697	Understanding and tuning these degradation susceptibilities are essential for advancing their applications in cardiovascular materials, orthopedic materials, and growth factor delivery applications.
34347454	3	49	dep	mimics	791:796	arg1	suspended					798:806	suspended	798:806	suspended in solution	798:818	This work evaluates the enzymatic stability of these PG mimics using hyaluronidases (I-S, IV-S, and II), chondroitinase ABC, and lysozyme, for PG mimics suspended in solution and adsorbed onto surfaces.
34347454	3	49	dep	mimics	791:796	arg1	adsorbed					824:831	adsorbed	824:831	adsorbed onto surfaces	824:845	This work evaluates the enzymatic stability of these PG mimics using hyaluronidases (I-S, IV-S, and II), chondroitinase ABC, and lysozyme, for PG mimics suspended in solution and adsorbed onto surfaces.
34347454	7	50	theme	polyelectrolyte	1156:1170	arg1	surfaces					1183:1190	polyelectrolyte multilayer surfaces	1156:1190	polyelectrolyte multilayer surfaces	1156:1190	Adsorption to polyelectrolyte multilayer surfaces protects PG mimics from degradation, compared to when PG mimics are combined with enzymes in solution; all surfaces are still intact after 21 days of enzyme exposure.
34347454	7	51	theme	PG	1246:1247	arg1	mimics					1249:1254	PG mimics	1246:1254	PG mimics	1246:1254	Adsorption to polyelectrolyte multilayer surfaces protects PG mimics from degradation, compared to when PG mimics are combined with enzymes in solution; all surfaces are still intact after 21 days of enzyme exposure.
34347454	4	52	theme	chondroitin	869:879	arg1	CS					890:891	CS	890:891	CS	890:891	Hyaluronan (HA)- and chondroitin sulfate (CS)-containing PG mimics are degraded by the hyaluronidases.
34347454	4	52	theme	chondroitin	869:879	arg1	sulfate					881:887	chondroitin sulfate	869:887	chondroitin sulfate (CS)	869:892	Hyaluronan (HA)- and chondroitin sulfate (CS)-containing PG mimics are degraded by the hyaluronidases.
34347454	3	53	theme	PG	698:699	arg1	mimics					701:706	these PG mimics	692:706	these PG mimics using hyaluronidases (I-S, IV-S, and II), chondroitinase ABC, and lysozyme	692:781	This work evaluates the enzymatic stability of these PG mimics using hyaluronidases (I-S, IV-S, and II), chondroitinase ABC, and lysozyme, for PG mimics suspended in solution and adsorbed onto surfaces.
34347454	9	54	from	applications	1636:1647	arg1	materials					1667:1675	cardiovascular materials	1652:1675	cardiovascular materials	1652:1675	Understanding and tuning these degradation susceptibilities are essential for advancing their applications in cardiovascular materials, orthopedic materials, and growth factor delivery applications.
34347454	1	55	theme	tissues	238:244	arg1	properties					224:233	the mechanical properties	209:233	the mechanical properties of tissues	209:244	Proteoglycans (PGs) play many important roles in biology, contributing to the mechanical properties of tissues, helping to organize extracellular matrix components, and participating in signaling mechanisms related to mechanotransduction, cell differentiation, immune responses, and wound healing.
34347454	5	56	theme	CS-	1016:1018	arg1	GCs					977:979	GCs	977:979	GCs	977:979	PCNs prepared with CS and GCs prepared with heparin are the only CS- and HA-containing PG mimics protected from chondroitinase ABC.
34347454	5	56	theme	CS-	1016:1018	arg1	mimics					1041:1046	the only CS- and HA-containing PG mimics	1007:1046	the only CS- and HA-containing PG mimics protected from chondroitinase ABC	1007:1080	PCNs prepared with CS and GCs prepared with heparin are the only CS- and HA-containing PG mimics protected from chondroitinase ABC.
34347454	5	56	theme	CS-	1016:1018	arg1	PCNs					951:954	PCNs	951:954	PCNs prepared with CS	951:971	PCNs prepared with CS and GCs prepared with heparin are the only CS- and HA-containing PG mimics protected from chondroitinase ABC.
34347454	8	57	dep	PG	1481:1482	arg1	mimic					1484:1488	mimic	1484:1488	mimic	1484:1488	This work reveals how the stability of PG mimics is controlled by both the composition and macromolecular assembly of the PG mimic and also by the size and specificity of the enzyme.
34347454	9	58	theme	growth	1704:1709	arg1	applications					1727:1738	growth factor delivery applications	1704:1738	growth factor delivery applications	1704:1738	Understanding and tuning these degradation susceptibilities are essential for advancing their applications in cardiovascular materials, orthopedic materials, and growth factor delivery applications.
34347454	9	59	from	materials	1689:1697	arg1	materials					1667:1675	cardiovascular materials	1652:1675	cardiovascular materials	1652:1675	Understanding and tuning these degradation susceptibilities are essential for advancing their applications in cardiovascular materials, orthopedic materials, and growth factor delivery applications.
34347454	1	60	theme	many	160:163	arg1	roles					175:179	many important roles	160:179	many important roles	160:179	Proteoglycans (PGs) play many important roles in biology, contributing to the mechanical properties of tissues, helping to organize extracellular matrix components, and participating in signaling mechanisms related to mechanotransduction, cell differentiation, immune responses, and wound healing.
34347454	8	61	theme	enzyme	1534:1539	arg1	specificity					1515:1525	specificity	1515:1525	specificity	1515:1525	This work reveals how the stability of PG mimics is controlled by both the composition and macromolecular assembly of the PG mimic and also by the size and specificity of the enzyme.
34347454	8	61	theme	enzyme	1534:1539	arg1	size					1506:1509	size	1506:1509	size	1506:1509	This work reveals how the stability of PG mimics is controlled by both the composition and macromolecular assembly of the PG mimic and also by the size and specificity of the enzyme.
34347454	7	62	theme	PG	1201:1202	arg1	mimics					1204:1209	PG mimics	1201:1209	PG mimics	1201:1209	Adsorption to polyelectrolyte multilayer surfaces protects PG mimics from degradation, compared to when PG mimics are combined with enzymes in solution; all surfaces are still intact after 21 days of enzyme exposure.
34347454	5	63	theme	chondroitinase	1063:1076	arg1	ABC					1078:1080	chondroitinase ABC	1063:1080	chondroitinase ABC	1063:1080	PCNs prepared with CS and GCs prepared with heparin are the only CS- and HA-containing PG mimics protected from chondroitinase ABC.
34347454	1	64	theme	important	165:173	arg1	roles					175:179	many important roles	160:179	many important roles	160:179	Proteoglycans (PGs) play many important roles in biology, contributing to the mechanical properties of tissues, helping to organize extracellular matrix components, and participating in signaling mechanisms related to mechanotransduction, cell differentiation, immune responses, and wound healing.
34347454	2	65	theme	PG-mimetic	537:546	arg1	copolymers					554:563	PG-mimetic graft copolymers	537:563	PG-mimetic graft copolymers	537:563	Our lab has designed two different types of PG mimics: polyelectrolyte complex nanoparticles (PCNs) and PG-mimetic graft copolymers (GCs), both of which are prepared using naturally occurring glycosaminoglycans.
32791274	8	0	theme	artificial	1007:1016	arg1	saliva					1018:1023	artificial saliva	1007:1023	artificial saliva	1007:1023	The in vitro morin release profiles in artificial saliva demonstrated that the microparticles controlled the release better (39.6%), followed by the film (41.1%) and the tablet (91.4%) after 20 h of testing.
32791274	4	1	theme	single-species	666:679	arg1	biofilms					681:688	single-species biofilms	666:688	single-species biofilms	666:688	Antibiofilm activity was analyzed for acidogenicity, microbial viability and the composition of the extracellular matrix of single-species biofilms.
32791274	6	2	from	porous	891:896	arg1	inner					867:871	inner	867:871	inner	867:871	The film and the tablet presented smooth and continuous surface and in the inner of the tablet was porous.
32791274	0	3	from	role	4:7	arg1	design					50:55	the design	46:55	the design of new drug delivery systems intended for antibiofilm activity of morin	46:127	The role of sodium alginate and gellan gum in the design of new drug delivery systems intended for antibiofilm activity of morin.
32791274	0	4	theme	drug	64:67	arg1	systems					78:84	new drug delivery systems	60:84	new drug delivery systems	60:84	The role of sodium alginate and gellan gum in the design of new drug delivery systems intended for antibiofilm activity of morin.
32791274	6	5	theme	tablet	880:885	arg1	inner					867:871	inner	867:871	inner	867:871	The film and the tablet presented smooth and continuous surface and in the inner of the tablet was porous.
32791274	9	6	attach	released	1186:1193	arg2	morin					1180:1184	The morin	1176:1184	The morin released from the systems	1176:1210	The morin released from the systems reduced the acidogenicity, microbial viability, concentration of insoluble extracellular polysaccharides and dry weight of biofilms, when compared to the control group.
32791274	9	6	attach	released	1186:1193	arg1	systems					1204:1210	the systems	1200:1210	the systems	1200:1210	The morin released from the systems reduced the acidogenicity, microbial viability, concentration of insoluble extracellular polysaccharides and dry weight of biofilms, when compared to the control group.
32791274	0	7	theme	new	60:62	arg1	systems					78:84	new drug delivery systems	60:84	new drug delivery systems	60:84	The role of sodium alginate and gellan gum in the design of new drug delivery systems intended for antibiofilm activity of morin.
32791274	9	8	theme	acidogenicity	1224:1236	arg1	viability					1249:1257	the acidogenicity, microbial viability	1220:1257	viability	1249:1257	The morin released from the systems reduced the acidogenicity, microbial viability, concentration of insoluble extracellular polysaccharides and dry weight of biofilms, when compared to the control group.
32791274	8	9	theme	in	972:973	arg1	profiles					995:1002	The in vitro morin release profiles	968:1002	The in vitro morin release profiles in artificial saliva	968:1023	The in vitro morin release profiles in artificial saliva demonstrated that the microparticles controlled the release better (39.6%), followed by the film (41.1%) and the tablet (91.4%) after 20 h of testing.
32791274	3	10	theme	mucoadhesion	502:513	arg1	terms					462:466	terms	462:466	terms of morphological characteristics, mucoadhesion and in vitro drug release	462:539	The systems were characterized in terms of morphological characteristics, mucoadhesion and in vitro drug release.
32791274	4	11	theme	Antibiofilm	542:552	arg1	activity					554:561	Antibiofilm activity	542:561	Antibiofilm activity	542:561	Antibiofilm activity was analyzed for acidogenicity, microbial viability and the composition of the extracellular matrix of single-species biofilms.
32791274	0	12	theme	systems	78:84	arg1	design					50:55	the design	46:55	the design of new drug delivery systems intended for antibiofilm activity of morin	46:127	The role of sodium alginate and gellan gum in the design of new drug delivery systems intended for antibiofilm activity of morin.
32791274	10	13	theme	study	1402:1406	arg1	findings					1385:1392	The findings	1381:1392	The findings of this study	1381:1406	The findings of this study showed that the morin has antibiofilm activity against cariogenic microorganisms.
32791274	3	14	theme	morphological	471:483	arg1	characteristics					485:499	morphological characteristics	471:499	morphological characteristics	471:499	The systems were characterized in terms of morphological characteristics, mucoadhesion and in vitro drug release.
32791274	9	15	theme	control	1366:1372	arg1	group					1374:1378	the control group	1362:1378	the control group	1362:1378	The morin released from the systems reduced the acidogenicity, microbial viability, concentration of insoluble extracellular polysaccharides and dry weight of biofilms, when compared to the control group.
32791274	0	16	theme	delivery	69:76	arg1	systems					78:84	new drug delivery systems	60:84	new drug delivery systems	60:84	The role of sodium alginate and gellan gum in the design of new drug delivery systems intended for antibiofilm activity of morin.
32791274	9	17	theme	microbial	1239:1247	arg1	viability					1249:1257	the acidogenicity, microbial viability	1220:1257	viability	1249:1257	The morin released from the systems reduced the acidogenicity, microbial viability, concentration of insoluble extracellular polysaccharides and dry weight of biofilms, when compared to the control group.
32791274	10	18	contain	has	1430:1432	arg1	morin					1424:1428	the morin	1420:1428	the morin	1420:1428	The findings of this study showed that the morin has antibiofilm activity against cariogenic microorganisms.
32791274	10	18	contain	has	1430:1432	arg2	activity					1446:1453	antibiofilm activity	1434:1453	antibiofilm activity against cariogenic microorganisms	1434:1487	The findings of this study showed that the morin has antibiofilm activity against cariogenic microorganisms.
32791274	8	19	theme	morin	981:985	arg1	profiles					995:1002	The in vitro morin release profiles	968:1002	The in vitro morin release profiles in artificial saliva	968:1023	The in vitro morin release profiles in artificial saliva demonstrated that the microparticles controlled the release better (39.6%), followed by the film (41.1%) and the tablet (91.4%) after 20 h of testing.
32791274	2	20	dep	alginate	321:328	arg1	gum					341:343	gum	341:343	gum	341:343	Microparticles, films and oral tablets based on alginate and gellan gum were developed also as a strategy to overcome the low aqueous solubility of morin.
32791274	2	21	theme	low	395:397	arg1	solubility					407:416	the low aqueous solubility	391:416	the low aqueous solubility of morin	391:425	Microparticles, films and oral tablets based on alginate and gellan gum were developed also as a strategy to overcome the low aqueous solubility of morin.
32791274	9	22	theme	insoluble	1277:1285	arg1	polysaccharides					1301:1315	insoluble extracellular polysaccharides	1277:1315	insoluble extracellular polysaccharides	1277:1315	The morin released from the systems reduced the acidogenicity, microbial viability, concentration of insoluble extracellular polysaccharides and dry weight of biofilms, when compared to the control group.
32791274	1	23	theme	controlled	141:150	arg1	systems					166:172	controlled drug delivery systems	141:172	controlled drug delivery systems	141:172	The use of controlled drug delivery systems represents an alternative and promising strategy for the use of antimicrobials in the oral cavity.
32791274	1	24	theme	antimicrobials	238:251	arg1	use					231:233	the use	227:233	the use of antimicrobials in the oral cavity	227:270	The use of controlled drug delivery systems represents an alternative and promising strategy for the use of antimicrobials in the oral cavity.
32791274	3	25	theme	drug	528:531	arg1	release					533:539	in vitro drug release	519:539	in vitro drug release	519:539	The systems were characterized in terms of morphological characteristics, mucoadhesion and in vitro drug release.
32791274	8	26	dep	in	972:973	arg1	vitro					975:979	vitro	975:979	vitro	975:979	The in vitro morin release profiles in artificial saliva demonstrated that the microparticles controlled the release better (39.6%), followed by the film (41.1%) and the tablet (91.4%) after 20 h of testing.
32791274	1	27	theme	drug	152:155	arg1	systems					166:172	controlled drug delivery systems	141:172	controlled drug delivery systems	141:172	The use of controlled drug delivery systems represents an alternative and promising strategy for the use of antimicrobials in the oral cavity.
32791274	0	28	theme	sodium	12:17	arg1	alginate					19:26	sodium alginate	12:26	sodium alginate	12:26	The role of sodium alginate and gellan gum in the design of new drug delivery systems intended for antibiofilm activity of morin.
32791274	1	29	from	use	231:233	arg1	cavity					265:270	the oral cavity	256:270	the oral cavity	256:270	The use of controlled drug delivery systems represents an alternative and promising strategy for the use of antimicrobials in the oral cavity.
32791274	8	30	theme	release	987:993	arg1	profiles					995:1002	The in vitro morin release profiles	968:1002	The in vitro morin release profiles in artificial saliva	968:1023	The in vitro morin release profiles in artificial saliva demonstrated that the microparticles controlled the release better (39.6%), followed by the film (41.1%) and the tablet (91.4%) after 20 h of testing.
32791274	1	31	theme	delivery	157:164	arg1	systems					166:172	controlled drug delivery systems	141:172	controlled drug delivery systems	141:172	The use of controlled drug delivery systems represents an alternative and promising strategy for the use of antimicrobials in the oral cavity.
32791274	3	32	theme	in	519:520	arg1	release					533:539	in vitro drug release	519:539	in vitro drug release	519:539	The systems were characterized in terms of morphological characteristics, mucoadhesion and in vitro drug release.
32791274	1	33	theme	systems	166:172	arg1	use					134:136	The use	130:136	The use of controlled drug delivery systems	130:172	The use of controlled drug delivery systems represents an alternative and promising strategy for the use of antimicrobials in the oral cavity.
32791274	2	34	theme	morin	421:425	arg1	solubility					407:416	the low aqueous solubility	391:416	the low aqueous solubility of morin	391:425	Microparticles, films and oral tablets based on alginate and gellan gum were developed also as a strategy to overcome the low aqueous solubility of morin.
32791274	1	35	theme	oral	260:263	arg1	cavity					265:270	the oral cavity	256:270	the oral cavity	256:270	The use of controlled drug delivery systems represents an alternative and promising strategy for the use of antimicrobials in the oral cavity.
32791274	5	36	theme	Scanning	691:698	arg1	Microscopy					709:718	Scanning Electron Microscopy	691:718	Scanning Electron Microscopy	691:718	Scanning Electron Microscopy demonstrated that the microparticles were spherical, rough and compact.
32791274	5	37	theme	Electron	700:707	arg1	Microscopy					709:718	Scanning Electron Microscopy	691:718	Scanning Electron Microscopy	691:718	Scanning Electron Microscopy demonstrated that the microparticles were spherical, rough and compact.
32791274	8	38	theme	testing	1167:1173	arg1	20 h					1159:1162	20 h	1159:1162	20 h of testing	1159:1173	The in vitro morin release profiles in artificial saliva demonstrated that the microparticles controlled the release better (39.6%), followed by the film (41.1%) and the tablet (91.4%) after 20 h of testing.
32791274	0	39	theme	alginate	19:26	arg1	role					4:7	The role	0:7	The role of sodium alginate and gellan gum in the design of new drug delivery systems intended for antibiofilm activity of morin.	0:128	The role of sodium alginate and gellan gum in the design of new drug delivery systems intended for antibiofilm activity of morin.
32791274	9	40	theme	extracellular	1287:1299	arg1	polysaccharides					1301:1315	insoluble extracellular polysaccharides	1277:1315	insoluble extracellular polysaccharides	1277:1315	The morin released from the systems reduced the acidogenicity, microbial viability, concentration of insoluble extracellular polysaccharides and dry weight of biofilms, when compared to the control group.
32791274	0	41	theme	antibiofilm	99:109	arg1	activity					111:118	antibiofilm activity	99:118	antibiofilm activity of morin	99:127	The role of sodium alginate and gellan gum in the design of new drug delivery systems intended for antibiofilm activity of morin.
32791274	9	42	theme	biofilms	1335:1342	arg1	weight					1325:1330	dry weight	1321:1330	dry weight of biofilms	1321:1342	The morin released from the systems reduced the acidogenicity, microbial viability, concentration of insoluble extracellular polysaccharides and dry weight of biofilms, when compared to the control group.
32791274	9	42	theme	biofilms	1335:1342	arg1	concentration					1260:1272	concentration	1260:1272	concentration of insoluble extracellular polysaccharides	1260:1315	The morin released from the systems reduced the acidogenicity, microbial viability, concentration of insoluble extracellular polysaccharides and dry weight of biofilms, when compared to the control group.
32791274	9	42	theme	biofilms	1335:1342	arg1	viability					1249:1257	the acidogenicity, microbial viability	1220:1257	viability	1249:1257	The morin released from the systems reduced the acidogenicity, microbial viability, concentration of insoluble extracellular polysaccharides and dry weight of biofilms, when compared to the control group.
32791274	10	43	theme	cariogenic	1463:1472	arg1	microorganisms					1474:1487	cariogenic microorganisms	1463:1487	cariogenic microorganisms	1463:1487	The findings of this study showed that the morin has antibiofilm activity against cariogenic microorganisms.
32791274	0	44	theme	gum	39:41	arg1	role					4:7	The role	0:7	The role of sodium alginate and gellan gum in the design of new drug delivery systems intended for antibiofilm activity of morin.	0:128	The role of sodium alginate and gellan gum in the design of new drug delivery systems intended for antibiofilm activity of morin.
32791274	9	45	theme	polysaccharides	1301:1315	arg1	weight					1325:1330	dry weight	1321:1330	dry weight of biofilms	1321:1342	The morin released from the systems reduced the acidogenicity, microbial viability, concentration of insoluble extracellular polysaccharides and dry weight of biofilms, when compared to the control group.
32791274	9	45	theme	polysaccharides	1301:1315	arg1	concentration					1260:1272	concentration	1260:1272	concentration of insoluble extracellular polysaccharides	1260:1315	The morin released from the systems reduced the acidogenicity, microbial viability, concentration of insoluble extracellular polysaccharides and dry weight of biofilms, when compared to the control group.
32791274	9	45	theme	polysaccharides	1301:1315	arg1	viability					1249:1257	the acidogenicity, microbial viability	1220:1257	viability	1249:1257	The morin released from the systems reduced the acidogenicity, microbial viability, concentration of insoluble extracellular polysaccharides and dry weight of biofilms, when compared to the control group.
32791274	0	46	theme	morin	123:127	arg1	activity					111:118	antibiofilm activity	99:118	antibiofilm activity of morin	99:127	The role of sodium alginate and gellan gum in the design of new drug delivery systems intended for antibiofilm activity of morin.
32791274	6	47	theme	continuous	837:846	arg1	surface					848:854	smooth and continuous surface	826:854	smooth and continuous surface	826:854	The film and the tablet presented smooth and continuous surface and in the inner of the tablet was porous.
32791274	3	48	theme	release	533:539	arg1	terms					462:466	terms	462:466	terms of morphological characteristics, mucoadhesion and in vitro drug release	462:539	The systems were characterized in terms of morphological characteristics, mucoadhesion and in vitro drug release.
32791274	8	49	from	profiles	995:1002	arg1	saliva					1018:1023	artificial saliva	1007:1023	artificial saliva	1007:1023	The in vitro morin release profiles in artificial saliva demonstrated that the microparticles controlled the release better (39.6%), followed by the film (41.1%) and the tablet (91.4%) after 20 h of testing.
32791274	0	50	theme	gellan	32:37	arg1	gum					39:41	gellan gum	32:41	gellan gum	32:41	The role of sodium alginate and gellan gum in the design of new drug delivery systems intended for antibiofilm activity of morin.
32791274	2	51	theme	aqueous	399:405	arg1	solubility					407:416	the low aqueous solubility	391:416	the low aqueous solubility of morin	391:425	Microparticles, films and oral tablets based on alginate and gellan gum were developed also as a strategy to overcome the low aqueous solubility of morin.
32791274	2	52	theme	oral	299:302	arg1	films					289:293	films	289:293	films	289:293	Microparticles, films and oral tablets based on alginate and gellan gum were developed also as a strategy to overcome the low aqueous solubility of morin.
32791274	2	52	theme	oral	299:302	arg1	tablets					304:310	oral tablets	299:310	oral tablets	299:310	Microparticles, films and oral tablets based on alginate and gellan gum were developed also as a strategy to overcome the low aqueous solubility of morin.
32791274	2	52	theme	oral	299:302	arg1	Microparticles					273:286	Microparticles	273:286	Microparticles	273:286	Microparticles, films and oral tablets based on alginate and gellan gum were developed also as a strategy to overcome the low aqueous solubility of morin.
32791274	2	52	theme	oral	299:302	arg1	strategy					370:377	a strategy	368:377	a strategy to overcome the low aqueous solubility of morin	368:425	Microparticles, films and oral tablets based on alginate and gellan gum were developed also as a strategy to overcome the low aqueous solubility of morin.
32791274	9	53	theme	dry	1321:1323	arg1	weight					1325:1330	dry weight	1321:1330	dry weight of biofilms	1321:1342	The morin released from the systems reduced the acidogenicity, microbial viability, concentration of insoluble extracellular polysaccharides and dry weight of biofilms, when compared to the control group.
32791274	10	54	theme	antibiofilm	1434:1444	arg1	activity					1446:1453	antibiofilm activity	1434:1453	antibiofilm activity against cariogenic microorganisms	1434:1487	The findings of this study showed that the morin has antibiofilm activity against cariogenic microorganisms.
32791274	6	55	theme	smooth	826:831	arg1	surface					848:854	smooth and continuous surface	826:854	smooth and continuous surface	826:854	The film and the tablet presented smooth and continuous surface and in the inner of the tablet was porous.
32791274	1	56	theme	alternative	188:198	arg1	strategy					214:221	an alternative and promising strategy	185:221	an alternative and promising strategy for the use of antimicrobials in the oral cavity	185:270	The use of controlled drug delivery systems represents an alternative and promising strategy for the use of antimicrobials in the oral cavity.
32791274	3	57	theme	characteristics	485:499	arg1	terms					462:466	terms	462:466	terms of morphological characteristics, mucoadhesion and in vitro drug release	462:539	The systems were characterized in terms of morphological characteristics, mucoadhesion and in vitro drug release.
32791274	8	58	dep	better	1085:1090	arg1	%					1097:1097	39.6%	1093:1097	39.6%	1093:1097	The in vitro morin release profiles in artificial saliva demonstrated that the microparticles controlled the release better (39.6%), followed by the film (41.1%) and the tablet (91.4%) after 20 h of testing.
32791274	4	59	theme	extracellular	642:654	arg1	matrix					656:661	the extracellular matrix	638:661	the extracellular matrix of single-species biofilms	638:688	Antibiofilm activity was analyzed for acidogenicity, microbial viability and the composition of the extracellular matrix of single-species biofilms.
32791274	4	60	theme	microbial	595:603	arg1	viability					605:613	microbial viability	595:613	microbial viability	595:613	Antibiofilm activity was analyzed for acidogenicity, microbial viability and the composition of the extracellular matrix of single-species biofilms.
32791274	4	61	theme	matrix	656:661	arg1	viability					605:613	microbial viability	595:613	microbial viability	595:613	Antibiofilm activity was analyzed for acidogenicity, microbial viability and the composition of the extracellular matrix of single-species biofilms.
32791274	4	61	theme	matrix	656:661	arg1	composition					623:633	the composition	619:633	the composition of the extracellular matrix of single-species biofilms	619:688	Antibiofilm activity was analyzed for acidogenicity, microbial viability and the composition of the extracellular matrix of single-species biofilms.
32791274	4	61	theme	matrix	656:661	arg1	acidogenicity					580:592	acidogenicity	580:592	acidogenicity	580:592	Antibiofilm activity was analyzed for acidogenicity, microbial viability and the composition of the extracellular matrix of single-species biofilms.
32791274	1	62	theme	promising	204:212	arg1	strategy					214:221	an alternative and promising strategy	185:221	an alternative and promising strategy for the use of antimicrobials in the oral cavity	185:270	The use of controlled drug delivery systems represents an alternative and promising strategy for the use of antimicrobials in the oral cavity.
32791274	3	63	dep	in	519:520	arg1	vitro					522:526	vitro	522:526	vitro	522:526	The systems were characterized in terms of morphological characteristics, mucoadhesion and in vitro drug release.
32791274	8	64	theme	better	1085:1090	arg1	release					1077:1083	the release	1073:1083	the release better (39.6%)	1073:1098	The in vitro morin release profiles in artificial saliva demonstrated that the microparticles controlled the release better (39.6%), followed by the film (41.1%) and the tablet (91.4%) after 20 h of testing.
32791274	4	65	theme	biofilms	681:688	arg1	matrix					656:661	the extracellular matrix	638:661	the extracellular matrix of single-species biofilms	638:688	Antibiofilm activity was analyzed for acidogenicity, microbial viability and the composition of the extracellular matrix of single-species biofilms.
32919547	1	0	from	issue	169:173	arg1	textile					185:191	textile	185:191	textile	185:191	Green treatment of natural fibres is a major issue in paper, textile and biocomposites industries to design innovative and eco-friendly products.
32919547	1	0	from	issue	169:173	arg1	paper					178:182	paper	178:182	paper	178:182	Green treatment of natural fibres is a major issue in paper, textile and biocomposites industries to design innovative and eco-friendly products.
32919547	1	0	from	issue	169:173	arg1	biocomposites					197:209	biocomposites	197:209	biocomposites	197:209	Green treatment of natural fibres is a major issue in paper, textile and biocomposites industries to design innovative and eco-friendly products.
32919547	3	1	theme	specific	463:470	arg1	area					480:483	high specific surface area	458:483	high specific surface area	458:483	Indeed, CNC have high mechanical properties, high specific surface area and great potential for functionalization.
32919547	3	1	theme	specific	463:470	arg1	properties					446:455	high mechanical properties	430:455	high mechanical properties	430:455	Indeed, CNC have high mechanical properties, high specific surface area and great potential for functionalization.
32919547	6	2	theme	plateau	875:881	arg1	values					883:888	plateau values	875:888	plateau values around 20 mg /gfibres for both	875:919	XG and CNC are homogeneously adsorbed on flax fabric and adsorption isotherms reach plateau values around 20 mg /gfibres for both.
32919547	3	3	theme	surface	472:478	arg1	area					480:483	high specific surface area	458:483	high specific surface area	458:483	Indeed, CNC have high mechanical properties, high specific surface area and great potential for functionalization.
32919547	3	3	theme	surface	472:478	arg1	properties					446:455	high mechanical properties	430:455	high mechanical properties	430:455	Indeed, CNC have high mechanical properties, high specific surface area and great potential for functionalization.
32919547	1	4	theme	eco-friendly	247:258	arg1	products					260:267	innovative and eco-friendly products	232:267	innovative and eco-friendly products	232:267	Green treatment of natural fibres is a major issue in paper, textile and biocomposites industries to design innovative and eco-friendly products.
32919547	4	5	theme	scanning	620:627	arg1	SEM					650:652	SEM	650:652	SEM	650:652	The adsorption of XG and CNC has been investigated in terms of localization by confocal and scanning electron microscopy (SEM) and quantification through adsorption isotherms.
32919547	4	5	theme	scanning	620:627	arg1	microscopy					638:647	confocal and scanning electron microscopy	607:647	microscopy	638:647	The adsorption of XG and CNC has been investigated in terms of localization by confocal and scanning electron microscopy (SEM) and quantification through adsorption isotherms.
32919547	5	6	theme	Adhesion	704:711	arg1	measurements					719:730	Adhesion force measurements	704:730	Adhesion force measurements	704:730	Adhesion force measurements have also been performed by Atomic Force Microscopy (AFM).
32919547	7	7	from	pre-adsorption	926:939	arg1	fabric					955:960	flax fabric	950:960	flax fabric	950:960	The pre-adsorption of XG on flax fabric influences the amount of adsorbed CNC in the high concentrations and also creates entanglements and strong interactions between XG and CNC with the formation of an extensible network.
32919547	4	8	theme	adsorption	682:691	arg1	isotherms					693:701	adsorption isotherms	682:701	adsorption isotherms	682:701	The adsorption of XG and CNC has been investigated in terms of localization by confocal and scanning electron microscopy (SEM) and quantification through adsorption isotherms.
32919547	0	9	theme	fibres	116:121	arg1	creation					78:85	the creation	74:85	the creation of hierarchically structured fibres	74:121	Adsorption of xyloglucan and cellulose nanocrystals on natural fibres for the creation of hierarchically structured fibres.
32919547	6	10	theme	adsorption	848:857	arg1	isotherms					859:867	adsorption isotherms	848:867	adsorption isotherms	848:867	XG and CNC are homogeneously adsorbed on flax fabric and adsorption isotherms reach plateau values around 20 mg /gfibres for both.
32919547	3	11	theme	great	489:493	arg1	potential					495:503	great potential	489:503	great potential for functionalization	489:525	Indeed, CNC have high mechanical properties, high specific surface area and great potential for functionalization.
32919547	3	11	theme	great	489:493	arg1	properties					446:455	high mechanical properties	430:455	high mechanical properties	430:455	Indeed, CNC have high mechanical properties, high specific surface area and great potential for functionalization.
32919547	1	12	theme	major	163:167	arg1	treatment					130:138	Green treatment	124:138	Green treatment of natural fibres	124:156	Green treatment of natural fibres is a major issue in paper, textile and biocomposites industries to design innovative and eco-friendly products.
32919547	1	12	theme	major	163:167	arg1	issue					169:173	a major issue	161:173	a major issue in paper, textile and biocomposites industries to design innovative and eco-friendly products	161:267	Green treatment of natural fibres is a major issue in paper, textile and biocomposites industries to design innovative and eco-friendly products.
32919547	0	13	theme	structured	105:114	arg1	fibres					116:121	hierarchically structured fibres	90:121	hierarchically structured fibres	90:121	Adsorption of xyloglucan and cellulose nanocrystals on natural fibres for the creation of hierarchically structured fibres.
32919547	0	14	from	nanocrystals	39:50	arg1	fibres					63:68	natural fibres	55:68	natural fibres for the creation of hierarchically structured fibres	55:121	Adsorption of xyloglucan and cellulose nanocrystals on natural fibres for the creation of hierarchically structured fibres.
32919547	7	15	theme	strong	1062:1067	arg1	interactions					1069:1080	strong interactions	1062:1080	strong interactions between XG and CNC with the formation of an extensible network	1062:1143	The pre-adsorption of XG on flax fabric influences the amount of adsorbed CNC in the high concentrations and also creates entanglements and strong interactions between XG and CNC with the formation of an extensible network.
32919547	2	16	dep	xyloglucan	352:361	arg1	CNC					396:398	CNC	396:398	CNC	396:398	In this work, hierarchical structuring of flax woven fabrics by the adsorption of xyloglucan (XG) and cellulose nanocrystals (CNC) is studied.
32919547	2	16	dep	xyloglucan	352:361	arg1	nanocrystals					382:393	nanocrystals	382:393	nanocrystals (CNC)	382:399	In this work, hierarchical structuring of flax woven fabrics by the adsorption of xyloglucan (XG) and cellulose nanocrystals (CNC) is studied.
32919547	5	17	theme	force	713:717	arg1	measurements					719:730	Adhesion force measurements	704:730	Adhesion force measurements	704:730	Adhesion force measurements have also been performed by Atomic Force Microscopy (AFM).
32919547	7	18	theme	extensible	1126:1135	arg1	network					1137:1143	an extensible network	1123:1143	an extensible network	1123:1143	The pre-adsorption of XG on flax fabric influences the amount of adsorbed CNC in the high concentrations and also creates entanglements and strong interactions between XG and CNC with the formation of an extensible network.
32919547	2	19	theme	flax	312:315	arg1	fabrics					323:329	flax woven fabrics	312:329	flax woven fabrics	312:329	In this work, hierarchical structuring of flax woven fabrics by the adsorption of xyloglucan (XG) and cellulose nanocrystals (CNC) is studied.
32919547	7	20	theme	network	1137:1143	arg1	formation					1110:1118	the formation	1106:1118	the formation of an extensible network	1106:1143	The pre-adsorption of XG on flax fabric influences the amount of adsorbed CNC in the high concentrations and also creates entanglements and strong interactions between XG and CNC with the formation of an extensible network.
32919547	4	21	theme	electron	629:636	arg1	SEM					650:652	SEM	650:652	SEM	650:652	The adsorption of XG and CNC has been investigated in terms of localization by confocal and scanning electron microscopy (SEM) and quantification through adsorption isotherms.
32919547	4	21	theme	electron	629:636	arg1	microscopy					638:647	confocal and scanning electron microscopy	607:647	microscopy	638:647	The adsorption of XG and CNC has been investigated in terms of localization by confocal and scanning electron microscopy (SEM) and quantification through adsorption isotherms.
32919547	7	22	with	entanglements	1044:1056	arg1	formation					1110:1118	the formation	1106:1118	the formation of an extensible network	1106:1143	The pre-adsorption of XG on flax fabric influences the amount of adsorbed CNC in the high concentrations and also creates entanglements and strong interactions between XG and CNC with the formation of an extensible network.
32919547	4	23	theme	localization	591:602	arg1	terms					582:586	terms	582:586	terms of localization by confocal and scanning electron microscopy (SEM) and quantification through adsorption isotherms	582:701	The adsorption of XG and CNC has been investigated in terms of localization by confocal and scanning electron microscopy (SEM) and quantification through adsorption isotherms.
32919547	0	24	theme	xyloglucan	14:23	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption of xyloglucan and cellulose	0:37	Adsorption of xyloglucan and cellulose nanocrystals on natural fibres for the creation of hierarchically structured fibres.
32919547	6	25	theme	flax	832:835	arg1	fabric					837:842	flax fabric	832:842	flax fabric	832:842	XG and CNC are homogeneously adsorbed on flax fabric and adsorption isotherms reach plateau values around 20 mg /gfibres for both.
32919547	2	26	theme	hierarchical	284:295	arg1	structuring					297:307	hierarchical structuring	284:307	hierarchical structuring of flax woven fabrics by the adsorption of xyloglucan (XG) and cellulose nanocrystals (CNC)	284:399	In this work, hierarchical structuring of flax woven fabrics by the adsorption of xyloglucan (XG) and cellulose nanocrystals (CNC) is studied.
32919547	4	27	theme	XG	546:547	arg1	adsorption					532:541	The adsorption	528:541	The adsorption of XG and CNC	528:555	The adsorption of XG and CNC has been investigated in terms of localization by confocal and scanning electron microscopy (SEM) and quantification through adsorption isotherms.
32919547	3	28	theme	high	430:433	arg1	area					480:483	high specific surface area	458:483	high specific surface area	458:483	Indeed, CNC have high mechanical properties, high specific surface area and great potential for functionalization.
32919547	3	28	theme	high	430:433	arg1	properties					446:455	high mechanical properties	430:455	high mechanical properties	430:455	Indeed, CNC have high mechanical properties, high specific surface area and great potential for functionalization.
32919547	3	28	theme	high	430:433	arg1	potential					495:503	great potential	489:503	great potential for functionalization	489:525	Indeed, CNC have high mechanical properties, high specific surface area and great potential for functionalization.
32919547	3	29	theme	high	458:461	arg1	area					480:483	high specific surface area	458:483	high specific surface area	458:483	Indeed, CNC have high mechanical properties, high specific surface area and great potential for functionalization.
32919547	3	29	theme	high	458:461	arg1	properties					446:455	high mechanical properties	430:455	high mechanical properties	430:455	Indeed, CNC have high mechanical properties, high specific surface area and great potential for functionalization.
32919547	7	30	theme	high	1007:1010	arg1	concentrations					1012:1025	the high concentrations	1003:1025	the high concentrations	1003:1025	The pre-adsorption of XG on flax fabric influences the amount of adsorbed CNC in the high concentrations and also creates entanglements and strong interactions between XG and CNC with the formation of an extensible network.
32919547	4	31	theme	confocal	607:614	arg1	SEM					650:652	SEM	650:652	SEM	650:652	The adsorption of XG and CNC has been investigated in terms of localization by confocal and scanning electron microscopy (SEM) and quantification through adsorption isotherms.
32919547	4	31	theme	confocal	607:614	arg1	microscopy					638:647	confocal and scanning electron microscopy	607:647	microscopy	638:647	The adsorption of XG and CNC has been investigated in terms of localization by confocal and scanning electron microscopy (SEM) and quantification through adsorption isotherms.
32919547	2	32	theme	cellulose	372:380	arg1	adsorption					338:347	the adsorption	334:347	the adsorption of xyloglucan (XG) and cellulose nanocrystals (CNC)	334:399	In this work, hierarchical structuring of flax woven fabrics by the adsorption of xyloglucan (XG) and cellulose nanocrystals (CNC) is studied.
32919547	0	33	theme	cellulose	29:37	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption of xyloglucan and cellulose	0:37	Adsorption of xyloglucan and cellulose nanocrystals on natural fibres for the creation of hierarchically structured fibres.
32919547	4	34	theme	quantification	659:672	arg1	terms					582:586	terms	582:586	terms of localization by confocal and scanning electron microscopy (SEM) and quantification through adsorption isotherms	582:701	The adsorption of XG and CNC has been investigated in terms of localization by confocal and scanning electron microscopy (SEM) and quantification through adsorption isotherms.
32919547	0	35	theme	natural	55:61	arg1	fibres					63:68	natural fibres	55:68	natural fibres for the creation of hierarchically structured fibres	55:121	Adsorption of xyloglucan and cellulose nanocrystals on natural fibres for the creation of hierarchically structured fibres.
32919547	3	36	contain	have	425:428	arg1	CNC					421:423	CNC	421:423	CNC	421:423	Indeed, CNC have high mechanical properties, high specific surface area and great potential for functionalization.
32919547	3	36	contain	have	425:428	arg2	area					480:483	high specific surface area	458:483	high specific surface area	458:483	Indeed, CNC have high mechanical properties, high specific surface area and great potential for functionalization.
32919547	3	36	contain	have	425:428	arg2	potential					495:503	great potential	489:503	great potential for functionalization	489:525	Indeed, CNC have high mechanical properties, high specific surface area and great potential for functionalization.
32919547	3	36	contain	have	425:428	arg2	properties					446:455	high mechanical properties	430:455	high mechanical properties	430:455	Indeed, CNC have high mechanical properties, high specific surface area and great potential for functionalization.
32919547	7	37	with	interactions	1069:1080	arg1	formation					1110:1118	the formation	1106:1118	the formation of an extensible network	1106:1143	The pre-adsorption of XG on flax fabric influences the amount of adsorbed CNC in the high concentrations and also creates entanglements and strong interactions between XG and CNC with the formation of an extensible network.
32919547	1	38	theme	fibres	151:156	arg1	treatment					130:138	Green treatment	124:138	Green treatment of natural fibres	124:156	Green treatment of natural fibres is a major issue in paper, textile and biocomposites industries to design innovative and eco-friendly products.
32919547	1	38	theme	fibres	151:156	arg1	issue					169:173	a major issue	161:173	a major issue in paper, textile and biocomposites industries to design innovative and eco-friendly products	161:267	Green treatment of natural fibres is a major issue in paper, textile and biocomposites industries to design innovative and eco-friendly products.
32919547	1	39	theme	Green	124:128	arg1	treatment					130:138	Green treatment	124:138	Green treatment of natural fibres	124:156	Green treatment of natural fibres is a major issue in paper, textile and biocomposites industries to design innovative and eco-friendly products.
32919547	1	39	theme	Green	124:128	arg1	issue					169:173	a major issue	161:173	a major issue in paper, textile and biocomposites industries to design innovative and eco-friendly products	161:267	Green treatment of natural fibres is a major issue in paper, textile and biocomposites industries to design innovative and eco-friendly products.
32919547	6	40	theme	20 mg	897:901	arg1	/gfibres					903:910	20 mg /gfibres	897:910	20 mg /gfibres	897:910	XG and CNC are homogeneously adsorbed on flax fabric and adsorption isotherms reach plateau values around 20 mg /gfibres for both.
32919547	7	41	theme	adsorbed	987:994	arg1	CNC					996:998	adsorbed CNC	987:998	adsorbed CNC	987:998	The pre-adsorption of XG on flax fabric influences the amount of adsorbed CNC in the high concentrations and also creates entanglements and strong interactions between XG and CNC with the formation of an extensible network.
32919547	5	42	theme	Atomic	760:765	arg1	AFM					785:787	AFM	785:787	AFM	785:787	Adhesion force measurements have also been performed by Atomic Force Microscopy (AFM).
32919547	5	42	theme	Atomic	760:765	arg1	Microscopy					773:782	Atomic Force Microscopy	760:782	Atomic Force Microscopy (AFM)	760:788	Adhesion force measurements have also been performed by Atomic Force Microscopy (AFM).
32919547	2	43	theme	fabrics	323:329	arg1	structuring					297:307	hierarchical structuring	284:307	hierarchical structuring of flax woven fabrics by the adsorption of xyloglucan (XG) and cellulose nanocrystals (CNC)	284:399	In this work, hierarchical structuring of flax woven fabrics by the adsorption of xyloglucan (XG) and cellulose nanocrystals (CNC) is studied.
32919547	7	44	theme	CNC	996:998	arg1	amount					977:982	the amount	973:982	the amount of adsorbed CNC	973:998	The pre-adsorption of XG on flax fabric influences the amount of adsorbed CNC in the high concentrations and also creates entanglements and strong interactions between XG and CNC with the formation of an extensible network.
32919547	7	44	theme	CNC	996:998	arg1	CNC					996:998	adsorbed CNC	987:998	adsorbed CNC	987:998	The pre-adsorption of XG on flax fabric influences the amount of adsorbed CNC in the high concentrations and also creates entanglements and strong interactions between XG and CNC with the formation of an extensible network.
32919547	7	45	theme	flax	950:953	arg1	fabric					955:960	flax fabric	950:960	flax fabric	950:960	The pre-adsorption of XG on flax fabric influences the amount of adsorbed CNC in the high concentrations and also creates entanglements and strong interactions between XG and CNC with the formation of an extensible network.
32919547	2	46	theme	woven	317:321	arg1	fabrics					323:329	flax woven fabrics	312:329	flax woven fabrics	312:329	In this work, hierarchical structuring of flax woven fabrics by the adsorption of xyloglucan (XG) and cellulose nanocrystals (CNC) is studied.
32919547	3	47	theme	mechanical	435:444	arg1	area					480:483	high specific surface area	458:483	high specific surface area	458:483	Indeed, CNC have high mechanical properties, high specific surface area and great potential for functionalization.
32919547	3	47	theme	mechanical	435:444	arg1	properties					446:455	high mechanical properties	430:455	high mechanical properties	430:455	Indeed, CNC have high mechanical properties, high specific surface area and great potential for functionalization.
32919547	3	47	theme	mechanical	435:444	arg1	potential					495:503	great potential	489:503	great potential for functionalization	489:525	Indeed, CNC have high mechanical properties, high specific surface area and great potential for functionalization.
32919547	1	48	theme	natural	143:149	arg1	fibres					151:156	natural fibres	143:156	natural fibres	143:156	Green treatment of natural fibres is a major issue in paper, textile and biocomposites industries to design innovative and eco-friendly products.
32919547	4	49	theme	CNC	553:555	arg1	adsorption					532:541	The adsorption	528:541	The adsorption of XG and CNC	528:555	The adsorption of XG and CNC has been investigated in terms of localization by confocal and scanning electron microscopy (SEM) and quantification through adsorption isotherms.
32919547	5	50	theme	Force	767:771	arg1	AFM					785:787	AFM	785:787	AFM	785:787	Adhesion force measurements have also been performed by Atomic Force Microscopy (AFM).
32919547	5	50	theme	Force	767:771	arg1	Microscopy					773:782	Atomic Force Microscopy	760:782	Atomic Force Microscopy (AFM)	760:788	Adhesion force measurements have also been performed by Atomic Force Microscopy (AFM).
32919547	1	51	dep	textile	185:191	arg1	design					225:230	design	225:230	to design innovative and eco-friendly products	222:267	Green treatment of natural fibres is a major issue in paper, textile and biocomposites industries to design innovative and eco-friendly products.
32919547	1	51	dep	textile	185:191	arg1	industries					211:220	industries	211:220	industries	211:220	Green treatment of natural fibres is a major issue in paper, textile and biocomposites industries to design innovative and eco-friendly products.
32919547	7	52	theme	XG	944:945	arg1	pre-adsorption					926:939	The pre-adsorption	922:939	The pre-adsorption of XG on flax fabric	922:960	The pre-adsorption of XG on flax fabric influences the amount of adsorbed CNC in the high concentrations and also creates entanglements and strong interactions between XG and CNC with the formation of an extensible network.
32919547	2	53	theme	xyloglucan	352:361	arg1	adsorption					338:347	the adsorption	334:347	the adsorption of xyloglucan (XG) and cellulose nanocrystals (CNC)	334:399	In this work, hierarchical structuring of flax woven fabrics by the adsorption of xyloglucan (XG) and cellulose nanocrystals (CNC) is studied.
32919547	1	54	theme	innovative	232:241	arg1	products					260:267	innovative and eco-friendly products	232:267	innovative and eco-friendly products	232:267	Green treatment of natural fibres is a major issue in paper, textile and biocomposites industries to design innovative and eco-friendly products.
32763407	2	0	theme	appropriate	534:544	arg1	conditions					564:573	the appropriate zeolite-treatment conditions	530:573	the appropriate zeolite-treatment conditions	530:573	In this respect, we present here Co2+-imprinted composite cryo-beads with switching on/off selectivity towards the template ions, engineered by selecting the appropriate zeolite-treatment conditions and/or controlling the initial sorption pH values.
32763407	2	1	attach	present	396:402	arg2	we					393:394	we	393:394	we	393:394	In this respect, we present here Co2+-imprinted composite cryo-beads with switching on/off selectivity towards the template ions, engineered by selecting the appropriate zeolite-treatment conditions and/or controlling the initial sorption pH values.
32763407	2	1	attach	present	396:402	arg1	respect					384:390	this respect	379:390	this respect	379:390	In this respect, we present here Co2+-imprinted composite cryo-beads with switching on/off selectivity towards the template ions, engineered by selecting the appropriate zeolite-treatment conditions and/or controlling the initial sorption pH values.
32763407	4	2	dep	7	840:840	arg1	up					834:835	up	834:835	up	834:835	The maximum sorption capacity values of ion-imprinted cryo-beads were from about 5 up to 7 times higher compared with those of non-imprinted ones.
32763407	4	2	dep	7	840:840	arg1	to					837:838	to	837:838	to	837:838	The maximum sorption capacity values of ion-imprinted cryo-beads were from about 5 up to 7 times higher compared with those of non-imprinted ones.
32763407	2	3	with	cryo-beads	434:443	arg1	selectivity					467:477	switching on/off selectivity	450:477	switching on/off selectivity towards the template ions	450:503	In this respect, we present here Co2+-imprinted composite cryo-beads with switching on/off selectivity towards the template ions, engineered by selecting the appropriate zeolite-treatment conditions and/or controlling the initial sorption pH values.
32763407	4	4	theme	capacity	772:779	arg1	values					781:786	The maximum sorption capacity values	751:786	The maximum sorption capacity values of ion-imprinted cryo-beads	751:814	The maximum sorption capacity values of ion-imprinted cryo-beads were from about 5 up to 7 times higher compared with those of non-imprinted ones.
32763407	4	4	theme	capacity	772:779	arg1	higher					848:853	higher	848:853	higher	848:853	The maximum sorption capacity values of ion-imprinted cryo-beads were from about 5 up to 7 times higher compared with those of non-imprinted ones.
32763407	0	5	theme	on/off	107:112	arg1	selectivity					114:124	on/off selectivity	107:124	on/off selectivity for heavy metal ions	107:145	Composite cryo-beads of chitosan reinforced with natural zeolites with remarkable elasticity and switching on/off selectivity for heavy metal ions.
32763407	5	6	dep	Cu2+	928:931	arg1	ions					954:957	ions	954:957	ions	954:957	Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form.
32763407	5	7	from	6	994:994	arg1	change					965:970	the change	961:970	the change of pH value from 4 to 6	961:994	Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form.
32763407	1	8	theme	composite	295:303	arg1	materials					313:321	composite polymer materials	295:321	composite polymer materials with 'desired' sorption properties and performances	295:373	Combining ion-imprinting technology with pH-dependent adsorptive features of acid- or salt-activated zeolites brings up the opportunity to develop composite polymer materials with 'desired' sorption properties and performances.
32763407	5	9	dep	6	994:994	arg1	to					991:992	to	991:992	to	991:992	Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form.
32763407	1	10	theme	polymer	305:311	arg1	materials					313:321	composite polymer materials	295:321	composite polymer materials with 'desired' sorption properties and performances	295:373	Combining ion-imprinting technology with pH-dependent adsorptive features of acid- or salt-activated zeolites brings up the opportunity to develop composite polymer materials with 'desired' sorption properties and performances.
32763407	6	11	theme	cryogel	1148:1154	arg1	matrix					1156:1161	cryogel matrix	1148:1161	cryogel matrix	1148:1161	The presence of zeolites within cryogel matrix generated composites with outstanding elasticity that allows the instant recovery of gels after full compression.
32763407	1	12	theme	pH-dependent	189:200	arg1	features					213:220	pH-dependent adsorptive features	189:220	pH-dependent adsorptive features of acid- or salt-activated zeolites	189:256	Combining ion-imprinting technology with pH-dependent adsorptive features of acid- or salt-activated zeolites brings up the opportunity to develop composite polymer materials with 'desired' sorption properties and performances.
32763407	5	13	from	H+-form	1091:1097	arg1	shift					1076:1080	the zeolite shift	1064:1080	the zeolite shift from the H+-form to the Na+-form	1064:1113	Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form.
32763407	2	14	theme	template	491:498	arg1	ions					500:503	the template ions	487:503	the template ions	487:503	In this respect, we present here Co2+-imprinted composite cryo-beads with switching on/off selectivity towards the template ions, engineered by selecting the appropriate zeolite-treatment conditions and/or controlling the initial sorption pH values.
32763407	1	15	theme	adsorptive	202:211	arg1	features					213:220	pH-dependent adsorptive features	189:220	pH-dependent adsorptive features of acid- or salt-activated zeolites	189:256	Combining ion-imprinting technology with pH-dependent adsorptive features of acid- or salt-activated zeolites brings up the opportunity to develop composite polymer materials with 'desired' sorption properties and performances.
32763407	5	16	theme	selectivity	1039:1049	arg1	quenching					1021:1029	a remarkable quenching	1008:1029	a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form	1008:1113	Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form.
32763407	5	17	theme	pH	975:976	arg1	value					978:982	pH value	975:982	pH value	975:982	Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form.
32763407	2	18	theme	Co2+-imprinted	409:422	arg1	cryo-beads					434:443	Co2+-imprinted composite cryo-beads	409:443	Co2+-imprinted composite cryo-beads	409:443	In this respect, we present here Co2+-imprinted composite cryo-beads with switching on/off selectivity towards the template ions, engineered by selecting the appropriate zeolite-treatment conditions and/or controlling the initial sorption pH values.
32763407	0	19	theme	Composite	0:8	arg1	cryo-beads					10:19	Composite cryo-beads	0:19	Composite cryo-beads of chitosan	0:31	Composite cryo-beads of chitosan reinforced with natural zeolites with remarkable elasticity and switching on/off selectivity for heavy metal ions.
32763407	2	20	theme	initial	598:604	arg1	values					618:623	the initial sorption pH values	594:623	the initial sorption pH values	594:623	In this respect, we present here Co2+-imprinted composite cryo-beads with switching on/off selectivity towards the template ions, engineered by selecting the appropriate zeolite-treatment conditions and/or controlling the initial sorption pH values.
32763407	1	21	with	materials	313:321	arg1	performances					362:373	performances	362:373	performances	362:373	Combining ion-imprinting technology with pH-dependent adsorptive features of acid- or salt-activated zeolites brings up the opportunity to develop composite polymer materials with 'desired' sorption properties and performances.
32763407	1	21	with	materials	313:321	arg1	properties					347:356	'desired' sorption properties	328:356	'desired' sorption properties	328:356	Combining ion-imprinting technology with pH-dependent adsorptive features of acid- or salt-activated zeolites brings up the opportunity to develop composite polymer materials with 'desired' sorption properties and performances.
32763407	5	22	theme	value	978:982	arg1	change					965:970	the change	961:970	the change of pH value from 4 to 6	961:994	Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form.
32763407	3	23	theme	zeolite	720:726	arg1	strategy					741:748	zeolite conditioning strategy	720:748	zeolite conditioning strategy	720:748	Co2+ chelating efficiency of all cryo-beads was investigated either at pH 4 or 6 depending on zeolite conditioning strategy.
32763407	0	24	theme	chitosan	24:31	arg1	cryo-beads					10:19	Composite cryo-beads	0:19	Composite cryo-beads of chitosan	0:31	Composite cryo-beads of chitosan reinforced with natural zeolites with remarkable elasticity and switching on/off selectivity for heavy metal ions.
32763407	7	25	theme	several	1389:1395	arg1	times					1397:1401	several times	1389:1401	several times without losing their features	1389:1431	These results indicate that the cryogel-type composites can be successfully re-used in separation processes for several times without losing their features.
32763407	0	26	theme	metal	136:140	arg1	ions					142:145	heavy metal ions	130:145	heavy metal ions	130:145	Composite cryo-beads of chitosan reinforced with natural zeolites with remarkable elasticity and switching on/off selectivity for heavy metal ions.
32763407	6	27	theme	gels	1248:1251	arg1	recovery					1236:1243	the instant recovery	1224:1243	the instant recovery of gels after full compression	1224:1274	The presence of zeolites within cryogel matrix generated composites with outstanding elasticity that allows the instant recovery of gels after full compression.
32763407	3	28	theme	Co2+	626:629	arg1	efficiency					641:650	Co2+ chelating efficiency	626:650	Co2+ chelating efficiency of all cryo-beads	626:668	Co2+ chelating efficiency of all cryo-beads was investigated either at pH 4 or 6 depending on zeolite conditioning strategy.
32763407	1	29	theme	acid-	225:229	arg1	zeolites					249:256	acid- or salt-activated zeolites	225:256	acid- or salt-activated zeolites	225:256	Combining ion-imprinting technology with pH-dependent adsorptive features of acid- or salt-activated zeolites brings up the opportunity to develop composite polymer materials with 'desired' sorption properties and performances.
32763407	0	30	theme	heavy	130:134	arg1	ions					142:145	heavy metal ions	130:145	heavy metal ions	130:145	Composite cryo-beads of chitosan reinforced with natural zeolites with remarkable elasticity and switching on/off selectivity for heavy metal ions.
32763407	3	31	theme	chelating	631:639	arg1	efficiency					641:650	Co2+ chelating efficiency	626:650	Co2+ chelating efficiency of all cryo-beads	626:668	Co2+ chelating efficiency of all cryo-beads was investigated either at pH 4 or 6 depending on zeolite conditioning strategy.
32763407	2	32	theme	on/off	460:465	arg1	selectivity					467:477	switching on/off selectivity	450:477	switching on/off selectivity towards the template ions	450:503	In this respect, we present here Co2+-imprinted composite cryo-beads with switching on/off selectivity towards the template ions, engineered by selecting the appropriate zeolite-treatment conditions and/or controlling the initial sorption pH values.
32763407	1	33	theme	desired	329:335	arg1	properties					347:356	'desired' sorption properties	328:356	'desired' sorption properties	328:356	Combining ion-imprinting technology with pH-dependent adsorptive features of acid- or salt-activated zeolites brings up the opportunity to develop composite polymer materials with 'desired' sorption properties and performances.
32763407	2	34	theme	pH	615:616	arg1	values					618:623	the initial sorption pH values	594:623	the initial sorption pH values	594:623	In this respect, we present here Co2+-imprinted composite cryo-beads with switching on/off selectivity towards the template ions, engineered by selecting the appropriate zeolite-treatment conditions and/or controlling the initial sorption pH values.
32763407	1	35	theme	salt-activated	234:247	arg1	zeolites					249:256	acid- or salt-activated zeolites	225:256	acid- or salt-activated zeolites	225:256	Combining ion-imprinting technology with pH-dependent adsorptive features of acid- or salt-activated zeolites brings up the opportunity to develop composite polymer materials with 'desired' sorption properties and performances.
32763407	5	36	theme	Co2+	1034:1037	arg1	selectivity					1039:1049	Co2+ selectivity	1034:1049	Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form	1034:1113	Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form.
32763407	7	37	theme	separation	1364:1373	arg1	processes					1375:1383	separation processes	1364:1383	separation processes	1364:1383	These results indicate that the cryogel-type composites can be successfully re-used in separation processes for several times without losing their features.
32763407	2	38	theme	switching	450:458	arg1	selectivity					467:477	switching on/off selectivity	450:477	switching on/off selectivity towards the template ions	450:503	In this respect, we present here Co2+-imprinted composite cryo-beads with switching on/off selectivity towards the template ions, engineered by selecting the appropriate zeolite-treatment conditions and/or controlling the initial sorption pH values.
32763407	2	39	theme	sorption	606:613	arg1	values					618:623	the initial sorption pH values	594:623	the initial sorption pH values	594:623	In this respect, we present here Co2+-imprinted composite cryo-beads with switching on/off selectivity towards the template ions, engineered by selecting the appropriate zeolite-treatment conditions and/or controlling the initial sorption pH values.
32763407	4	40	theme	non-imprinted	878:890	arg1	ones					892:895	non-imprinted ones	878:895	non-imprinted ones	878:895	The maximum sorption capacity values of ion-imprinted cryo-beads were from about 5 up to 7 times higher compared with those of non-imprinted ones.
32763407	1	41	theme	zeolites	249:256	arg1	features					213:220	pH-dependent adsorptive features	189:220	pH-dependent adsorptive features of acid- or salt-activated zeolites	189:256	Combining ion-imprinting technology with pH-dependent adsorptive features of acid- or salt-activated zeolites brings up the opportunity to develop composite polymer materials with 'desired' sorption properties and performances.
32763407	1	42	theme	sorption	338:345	arg1	properties					347:356	'desired' sorption properties	328:356	'desired' sorption properties	328:356	Combining ion-imprinting technology with pH-dependent adsorptive features of acid- or salt-activated zeolites brings up the opportunity to develop composite polymer materials with 'desired' sorption properties and performances.
32763407	3	43	dep	pH 4	697:700	arg1	either					687:692	either	687:692	either	687:692	Co2+ chelating efficiency of all cryo-beads was investigated either at pH 4 or 6 depending on zeolite conditioning strategy.
32763407	4	44	theme	ion-imprinted	791:803	arg1	cryo-beads					805:814	ion-imprinted cryo-beads	791:814	ion-imprinted cryo-beads	791:814	The maximum sorption capacity values of ion-imprinted cryo-beads were from about 5 up to 7 times higher compared with those of non-imprinted ones.
32763407	0	45	theme	natural	49:55	arg1	zeolites					57:64	natural zeolites	49:64	natural zeolites with remarkable elasticity	49:91	Composite cryo-beads of chitosan reinforced with natural zeolites with remarkable elasticity and switching on/off selectivity for heavy metal ions.
32763407	6	46	theme	full	1259:1262	arg1	compression					1264:1274	full compression	1259:1274	full compression	1259:1274	The presence of zeolites within cryogel matrix generated composites with outstanding elasticity that allows the instant recovery of gels after full compression.
32763407	3	47	theme	cryo-beads	659:668	arg1	efficiency					641:650	Co2+ chelating efficiency	626:650	Co2+ chelating efficiency of all cryo-beads	626:668	Co2+ chelating efficiency of all cryo-beads was investigated either at pH 4 or 6 depending on zeolite conditioning strategy.
32763407	7	48	theme	cryogel-type	1309:1320	arg1	composites					1322:1331	the cryogel-type composites	1305:1331	the cryogel-type composites	1305:1331	These results indicate that the cryogel-type composites can be successfully re-used in separation processes for several times without losing their features.
32763407	6	49	with	composites	1173:1182	arg1	elasticity					1201:1210	outstanding elasticity	1189:1210	outstanding elasticity	1189:1210	The presence of zeolites within cryogel matrix generated composites with outstanding elasticity that allows the instant recovery of gels after full compression.
32763407	5	50	theme	zeolite	1068:1074	arg1	shift					1076:1080	the zeolite shift	1064:1080	the zeolite shift from the H+-form to the Na+-form	1064:1113	Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form.
32763407	4	51	theme	maximum	755:761	arg1	values					781:786	The maximum sorption capacity values	751:786	The maximum sorption capacity values of ion-imprinted cryo-beads	751:814	The maximum sorption capacity values of ion-imprinted cryo-beads were from about 5 up to 7 times higher compared with those of non-imprinted ones.
32763407	4	51	theme	maximum	755:761	arg1	higher					848:853	higher	848:853	higher	848:853	The maximum sorption capacity values of ion-imprinted cryo-beads were from about 5 up to 7 times higher compared with those of non-imprinted ones.
32763407	6	52	theme	zeolites	1132:1139	arg1	presence					1120:1127	The presence	1116:1127	The presence of zeolites within cryogel matrix	1116:1161	The presence of zeolites within cryogel matrix generated composites with outstanding elasticity that allows the instant recovery of gels after full compression.
32763407	6	53	theme	outstanding	1189:1199	arg1	elasticity					1201:1210	outstanding elasticity	1189:1210	outstanding elasticity	1189:1210	The presence of zeolites within cryogel matrix generated composites with outstanding elasticity that allows the instant recovery of gels after full compression.
32763407	3	54	theme	conditioning	728:739	arg1	strategy					741:748	zeolite conditioning strategy	720:748	zeolite conditioning strategy	720:748	Co2+ chelating efficiency of all cryo-beads was investigated either at pH 4 or 6 depending on zeolite conditioning strategy.
32763407	5	55	theme	remarkable	1010:1019	arg1	quenching					1021:1029	a remarkable quenching	1008:1029	a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form	1008:1113	Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form.
32763407	0	56	with	zeolites	57:64	arg1	elasticity					82:91	remarkable elasticity	71:91	remarkable elasticity	71:91	Composite cryo-beads of chitosan reinforced with natural zeolites with remarkable elasticity and switching on/off selectivity for heavy metal ions.
32763407	4	57	theme	cryo-beads	805:814	arg1	values					781:786	The maximum sorption capacity values	751:786	The maximum sorption capacity values of ion-imprinted cryo-beads	751:814	The maximum sorption capacity values of ion-imprinted cryo-beads were from about 5 up to 7 times higher compared with those of non-imprinted ones.
32763407	4	57	theme	cryo-beads	805:814	arg1	higher					848:853	higher	848:853	higher	848:853	The maximum sorption capacity values of ion-imprinted cryo-beads were from about 5 up to 7 times higher compared with those of non-imprinted ones.
32763407	0	58	theme	remarkable	71:80	arg1	elasticity					82:91	remarkable elasticity	71:91	remarkable elasticity	71:91	Composite cryo-beads of chitosan reinforced with natural zeolites with remarkable elasticity and switching on/off selectivity for heavy metal ions.
32763407	6	59	theme	instant	1228:1234	arg1	recovery					1236:1243	the instant recovery	1224:1243	the instant recovery of gels after full compression	1224:1274	The presence of zeolites within cryogel matrix generated composites with outstanding elasticity that allows the instant recovery of gels after full compression.
32763407	5	60	theme	competitive	904:914	arg1	Ni2+					934:937	Ni2+	934:937	Ni2+	934:937	Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form.
32763407	5	60	theme	competitive	904:914	arg1	Cu2+					928:931	Cu2+	928:931	Cu2+	928:931	Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form.
32763407	5	60	theme	competitive	904:914	arg1	Cd2+					949:952	Cd2+	949:952	Cd2+	949:952	Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form.
32763407	5	60	theme	competitive	904:914	arg1	conditions					916:925	competitive conditions	904:925	competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions)	904:958	Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form.
32763407	5	60	theme	competitive	904:914	arg1	Fe2+					940:943	Fe2+	940:943	Fe2+	940:943	Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form.
32763407	4	61	theme	sorption	763:770	arg1	values					781:786	The maximum sorption capacity values	751:786	The maximum sorption capacity values of ion-imprinted cryo-beads	751:814	The maximum sorption capacity values of ion-imprinted cryo-beads were from about 5 up to 7 times higher compared with those of non-imprinted ones.
32763407	4	61	theme	sorption	763:770	arg1	higher					848:853	higher	848:853	higher	848:853	The maximum sorption capacity values of ion-imprinted cryo-beads were from about 5 up to 7 times higher compared with those of non-imprinted ones.
32763407	2	62	theme	composite	424:432	arg1	cryo-beads					434:443	Co2+-imprinted composite cryo-beads	409:443	Co2+-imprinted composite cryo-beads	409:443	In this respect, we present here Co2+-imprinted composite cryo-beads with switching on/off selectivity towards the template ions, engineered by selecting the appropriate zeolite-treatment conditions and/or controlling the initial sorption pH values.
32763407	2	63	theme	zeolite-treatment	546:562	arg1	conditions					564:573	the appropriate zeolite-treatment conditions	530:573	the appropriate zeolite-treatment conditions	530:573	In this respect, we present here Co2+-imprinted composite cryo-beads with switching on/off selectivity towards the template ions, engineered by selecting the appropriate zeolite-treatment conditions and/or controlling the initial sorption pH values.
32763407	5	64	dep	conditions	916:925	arg1	conditions					916:925	competitive conditions	904:925	competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions)	904:958	Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form.
32763407	5	64	dep	conditions	916:925	arg1	Ni2+					934:937	Ni2+	934:937	Ni2+	934:937	Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form.
32763407	5	64	dep	conditions	916:925	arg1	Cu2+					928:931	Cu2+	928:931	Cu2+	928:931	Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form.
32763407	5	64	dep	conditions	916:925	arg1	Cd2+					949:952	Cd2+	949:952	Cd2+	949:952	Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form.
32763407	5	64	dep	conditions	916:925	arg1	Fe2+					940:943	Fe2+	940:943	Fe2+	940:943	Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form.
32763407	1	65	theme	ion-imprinting	158:171	arg1	technology					173:182	ion-imprinting technology	158:182	ion-imprinting technology	158:182	Combining ion-imprinting technology with pH-dependent adsorptive features of acid- or salt-activated zeolites brings up the opportunity to develop composite polymer materials with 'desired' sorption properties and performances.
33901554	7	0	theme	addition	1348:1355	arg1	level					1357:1361	addition level	1348:1361	addition level of host material	1348:1378	For M.SNPs, k value was decreased from 13.097 to 2.938 as addition level of host material increased from 0.5 to 2.5%.
33901554	1	1	theme	absolute	146:153	arg1	solution					174:181	absolute ethanolic curcumin solution	146:181	absolute ethanolic curcumin solution with various content (300-1200 μg)	146:216	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	1	2	theme	aqueous	231:237	arg1	dispersion					239:248	aqueous dispersion	231:248	aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%)	231:335	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	0	3	theme	curcumin	75:82	arg1	encapsulation					58:70	encapsulation	58:70	encapsulation of curcumin	58:82	Application of starch nanoparticles as host materials for encapsulation of curcumin: Effect of citric acid modification.
33901554	1	4	theme	gas	397:399	arg1	purge					401:405	nitrogen gas purge	388:405	nitrogen gas purge	388:405	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	1	5	theme	ethanolic	155:163	arg1	solution					174:181	absolute ethanolic curcumin solution	146:181	absolute ethanolic curcumin solution with various content (300-1200 μg)	146:216	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	3	6	theme	major	755:759	arg1	forces					769:774	the major driving forces	751:774	the major driving forces for encapsulation	751:792	The XRD results revealed that curcumin was amorphously encapsulated in the composite, and hydrogen bond formation between M.SNPs and curcumin was one of the major driving forces for encapsulation as suggested by FT-IR.
33901554	7	7	theme	k	1302:1302	arg1	value					1304:1308	k value	1302:1308	k value	1302:1308	For M.SNPs, k value was decreased from 13.097 to 2.938 as addition level of host material increased from 0.5 to 2.5%.
33901554	3	8	theme	driving	761:767	arg1	forces					769:774	the major driving forces	751:774	the major driving forces for encapsulation	751:792	The XRD results revealed that curcumin was amorphously encapsulated in the composite, and hydrogen bond formation between M.SNPs and curcumin was one of the major driving forces for encapsulation as suggested by FT-IR.
33901554	2	9	theme	curcumin	496:503	arg1	μg					490:491	75.7 μg	485:491	75.7 μg of curcumin	485:503	SNPs (100 mg) could encapsulate 75.7 μg of curcumin in matrices of the composite, while 100 mg of M.SNPs could encapsulate 144.9 μg of curcumin.
33901554	1	10	theme	citric	253:258	arg1	nanoparticles					281:293	citric acid-modified starch nanoparticles	253:293	citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%)	253:335	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	1	10	theme	citric	253:258	arg1	M.SNPs					296:301	M.SNPs	296:301	M.SNPs	296:301	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	0	11	theme	acid	102:105	arg1	modification					107:118	citric acid modification	95:118	citric acid modification	95:118	Application of starch nanoparticles as host materials for encapsulation of curcumin: Effect of citric acid modification.
33901554	4	12	dep	255.3	923:927	arg1	to					920:921	to	920:921	to	920:921	The composites had a spherical shape and mean particle size of the composites was increased from 136.3 to 255.3 nm with higher curcumin content in the matrices of composites.
33901554	1	13	theme	curcumin	165:172	arg1	solution					174:181	absolute ethanolic curcumin solution	146:181	absolute ethanolic curcumin solution with various content (300-1200 μg)	146:216	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	0	14	theme	citric	95:100	arg1	modification					107:118	citric acid modification	95:118	citric acid modification	95:118	Application of starch nanoparticles as host materials for encapsulation of curcumin: Effect of citric acid modification.
33901554	3	15	theme	XRD	602:604	arg1	results					606:612	The XRD results	598:612	The XRD results	598:612	The XRD results revealed that curcumin was amorphously encapsulated in the composite, and hydrogen bond formation between M.SNPs and curcumin was one of the major driving forces for encapsulation as suggested by FT-IR.
33901554	6	16	theme	intestinal	1207:1216	arg1	digestion					1224:1232	simulated intestinal fluid digestion	1197:1232	simulated intestinal fluid digestion	1197:1232	For the release of curcumin in simulated intestinal fluid digestion, release mechanism explained by Korsmeyer-Peppas model.
33901554	1	17	with	nanoparticles	281:293	arg1	contents					317:324	various contents	309:324	various contents (0.5-2.5%)	309:335	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	1	17	with	nanoparticles	281:293	arg1	%					334:334	0.5-2.5%	327:334	0.5-2.5%	327:334	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	6	18	theme	fluid	1218:1222	arg1	digestion					1224:1232	simulated intestinal fluid digestion	1197:1232	simulated intestinal fluid digestion	1197:1232	For the release of curcumin in simulated intestinal fluid digestion, release mechanism explained by Korsmeyer-Peppas model.
33901554	4	19	theme	particle	863:870	arg1	size					872:875	mean particle size	858:875	mean particle size of the composites	858:893	The composites had a spherical shape and mean particle size of the composites was increased from 136.3 to 255.3 nm with higher curcumin content in the matrices of composites.
33901554	2	20	theme	composite	524:532	arg1	matrices					508:515	matrices	508:515	matrices of the composite	508:532	SNPs (100 mg) could encapsulate 75.7 μg of curcumin in matrices of the composite, while 100 mg of M.SNPs could encapsulate 144.9 μg of curcumin.
33901554	5	21	theme	curcumin	1025:1032	arg1	stability					1012:1020	thermal stability	1004:1020	thermal stability	1004:1020	UV, pH, and thermal stability of curcumin significantly enhanced by the encapsulation, which was further increased when using M.SNPs and/or higher content of host materials.
33901554	5	21	theme	curcumin	1025:1032	arg1	pH					996:997	pH	996:997	pH	996:997	UV, pH, and thermal stability of curcumin significantly enhanced by the encapsulation, which was further increased when using M.SNPs and/or higher content of host materials.
33901554	5	21	theme	curcumin	1025:1032	arg1	UV					992:993	UV	992:993	UV	992:993	UV, pH, and thermal stability of curcumin significantly enhanced by the encapsulation, which was further increased when using M.SNPs and/or higher content of host materials.
33901554	1	22	theme	acid-modified	260:272	arg1	nanoparticles					281:293	citric acid-modified starch nanoparticles	253:293	citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%)	253:335	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	1	22	theme	acid-modified	260:272	arg1	M.SNPs					296:301	M.SNPs	296:301	M.SNPs	296:301	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	1	23	theme	ethanol	419:425	arg1	content					427:433	ethanol content	419:433	ethanol content decreased to 1%	419:449	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	7	24	dep	2.5	1402:1404	arg1	to					1399:1400	to	1399:1400	to	1399:1400	For M.SNPs, k value was decreased from 13.097 to 2.938 as addition level of host material increased from 0.5 to 2.5%.
33901554	1	25	theme	various	188:194	arg1	content					196:202	various content	188:202	various content (300-1200 μg)	188:216	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	1	25	theme	various	188:194	arg1	μg					214:215	300-1200 μg	205:215	300-1200 μg	205:215	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	0	26	theme	nanoparticles	22:34	arg1	Application					0:10	Application	0:10	Application of starch nanoparticles as host materials for encapsulation of curcumin: Effect of citric acid modification.	0:119	Application of starch nanoparticles as host materials for encapsulation of curcumin: Effect of citric acid modification.
33901554	1	27	theme	starch	274:279	arg1	nanoparticles					281:293	citric acid-modified starch nanoparticles	253:293	citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%)	253:335	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	1	27	theme	starch	274:279	arg1	M.SNPs					296:301	M.SNPs	296:301	M.SNPs	296:301	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	6	28	theme	simulated	1197:1205	arg1	digestion					1224:1232	simulated intestinal fluid digestion	1197:1232	simulated intestinal fluid digestion	1197:1232	For the release of curcumin in simulated intestinal fluid digestion, release mechanism explained by Korsmeyer-Peppas model.
33901554	6	29	theme	release	1235:1241	arg1	mechanism					1243:1251	release mechanism	1235:1251	release mechanism	1235:1251	For the release of curcumin in simulated intestinal fluid digestion, release mechanism explained by Korsmeyer-Peppas model.
33901554	2	30	theme	M.SNPs	551:556	arg1	mg					545:546	100 mg	541:546	100 mg of M.SNPs	541:556	SNPs (100 mg) could encapsulate 75.7 μg of curcumin in matrices of the composite, while 100 mg of M.SNPs could encapsulate 144.9 μg of curcumin.
33901554	0	31	theme	starch	15:20	arg1	nanoparticles					22:34	starch nanoparticles	15:34	starch nanoparticles as host materials for encapsulation of curcumin	15:82	Application of starch nanoparticles as host materials for encapsulation of curcumin: Effect of citric acid modification.
33901554	1	32	theme	nanoparticles	281:293	arg1	dispersion					239:248	aqueous dispersion	231:248	aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%)	231:335	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	0	33	theme	modification	107:118	arg1	Effect					85:90	Effect	85:90	Application of starch nanoparticles as host materials for encapsulation of curcumin: Effect of citric acid modification.	0:119	Application of starch nanoparticles as host materials for encapsulation of curcumin: Effect of citric acid modification.
33901554	5	34	theme	higher	1132:1137	arg1	content					1139:1145	higher content	1132:1145	higher content of host materials	1132:1163	UV, pH, and thermal stability of curcumin significantly enhanced by the encapsulation, which was further increased when using M.SNPs and/or higher content of host materials.
33901554	0	35	theme	host	39:42	arg1	materials					44:52	host materials	39:52	host materials for encapsulation of curcumin	39:82	Application of starch nanoparticles as host materials for encapsulation of curcumin: Effect of citric acid modification.
33901554	6	36	theme	curcumin	1185:1192	arg1	release					1174:1180	the release	1170:1180	the release of curcumin in simulated intestinal fluid digestion	1170:1232	For the release of curcumin in simulated intestinal fluid digestion, release mechanism explained by Korsmeyer-Peppas model.
33901554	4	37	theme	higher	937:942	arg1	content					953:959	higher curcumin content	937:959	higher curcumin content in the matrices of composites	937:989	The composites had a spherical shape and mean particle size of the composites was increased from 136.3 to 255.3 nm with higher curcumin content in the matrices of composites.
33901554	4	38	theme	composites	980:989	arg1	matrices					968:975	the matrices	964:975	the matrices of composites	964:989	The composites had a spherical shape and mean particle size of the composites was increased from 136.3 to 255.3 nm with higher curcumin content in the matrices of composites.
33901554	4	39	theme	spherical	838:846	arg1	shape					848:852	a spherical shape	836:852	a spherical shape	836:852	The composites had a spherical shape and mean particle size of the composites was increased from 136.3 to 255.3 nm with higher curcumin content in the matrices of composites.
33901554	5	40	theme	thermal	1004:1010	arg1	stability					1012:1020	thermal stability	1004:1020	thermal stability	1004:1020	UV, pH, and thermal stability of curcumin significantly enhanced by the encapsulation, which was further increased when using M.SNPs and/or higher content of host materials.
33901554	1	41	theme	mixture	362:368	arg1	ethanol					347:353	ethanol	347:353	ethanol of the mixture	347:368	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	4	42	theme	composites	884:893	arg1	size					872:875	mean particle size	858:875	mean particle size of the composites	858:893	The composites had a spherical shape and mean particle size of the composites was increased from 136.3 to 255.3 nm with higher curcumin content in the matrices of composites.
33901554	6	43	from	release	1174:1180	arg1	digestion					1224:1232	simulated intestinal fluid digestion	1197:1232	simulated intestinal fluid digestion	1197:1232	For the release of curcumin in simulated intestinal fluid digestion, release mechanism explained by Korsmeyer-Peppas model.
33901554	7	44	theme	host	1366:1369	arg1	material					1371:1378	host material	1366:1378	host material	1366:1378	For M.SNPs, k value was decreased from 13.097 to 2.938 as addition level of host material increased from 0.5 to 2.5%.
33901554	2	45	theme	curcumin	588:595	arg1	μg					582:583	144.9 μg	576:583	144.9 μg of curcumin	576:595	SNPs (100 mg) could encapsulate 75.7 μg of curcumin in matrices of the composite, while 100 mg of M.SNPs could encapsulate 144.9 μg of curcumin.
33901554	7	46	dep	2.938	1339:1343	arg1	to					1336:1337	to	1336:1337	to	1336:1337	For M.SNPs, k value was decreased from 13.097 to 2.938 as addition level of host material increased from 0.5 to 2.5%.
33901554	1	47	theme	various	309:315	arg1	contents					317:324	various contents	309:324	various contents (0.5-2.5%)	309:335	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	1	47	theme	various	309:315	arg1	%					334:334	0.5-2.5%	327:334	0.5-2.5%	327:334	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	7	48	theme	material	1371:1378	arg1	level					1357:1361	addition level	1348:1361	addition level of host material	1348:1378	For M.SNPs, k value was decreased from 13.097 to 2.938 as addition level of host material increased from 0.5 to 2.5%.
33901554	1	49	dep	evaporated	374:383	arg1	content					427:433	ethanol content	419:433	ethanol content decreased to 1%	419:449	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	4	50	theme	curcumin	944:951	arg1	content					953:959	higher curcumin content	937:959	higher curcumin content in the matrices of composites	937:989	The composites had a spherical shape and mean particle size of the composites was increased from 136.3 to 255.3 nm with higher curcumin content in the matrices of composites.
33901554	3	51	theme	bond	697:700	arg1	formation					702:710	hydrogen bond formation	688:710	hydrogen bond formation between M.SNPs and curcumin	688:738	The XRD results revealed that curcumin was amorphously encapsulated in the composite, and hydrogen bond formation between M.SNPs and curcumin was one of the major driving forces for encapsulation as suggested by FT-IR.
33901554	1	52	with	solution	174:181	arg1	content					196:202	various content	188:202	various content (300-1200 μg)	188:216	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	1	52	with	solution	174:181	arg1	μg					214:215	300-1200 μg	205:215	300-1200 μg	205:215	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	4	53	contain	had	832:834	arg2	shape					848:852	a spherical shape	836:852	a spherical shape	836:852	The composites had a spherical shape and mean particle size of the composites was increased from 136.3 to 255.3 nm with higher curcumin content in the matrices of composites.
33901554	4	53	contain	had	832:834	arg1	composites					821:830	The composites	817:830	The composites	817:830	The composites had a spherical shape and mean particle size of the composites was increased from 136.3 to 255.3 nm with higher curcumin content in the matrices of composites.
33901554	5	54	theme	host	1150:1153	arg1	materials					1155:1163	host materials	1150:1163	host materials	1150:1163	UV, pH, and thermal stability of curcumin significantly enhanced by the encapsulation, which was further increased when using M.SNPs and/or higher content of host materials.
33901554	4	55	from	content	953:959	arg1	matrices					968:975	the matrices	964:975	the matrices of composites	964:989	The composites had a spherical shape and mean particle size of the composites was increased from 136.3 to 255.3 nm with higher curcumin content in the matrices of composites.
33901554	4	56	theme	mean	858:861	arg1	size					872:875	mean particle size	858:875	mean particle size of the composites	858:893	The composites had a spherical shape and mean particle size of the composites was increased from 136.3 to 255.3 nm with higher curcumin content in the matrices of composites.
33901554	1	57	theme	nitrogen	388:395	arg1	purge					401:405	nitrogen gas purge	388:405	nitrogen gas purge	388:405	To encapsulate curcumin, absolute ethanolic curcumin solution with various content (300-1200 μg) was added to aqueous dispersion of citric acid-modified starch nanoparticles (M.SNPs) with various contents (0.5-2.5%), and then ethanol of the mixture was evaporated by nitrogen gas purge for 40 min (ethanol content decreased to 1%).
33901554	6	58	theme	Korsmeyer-Peppas	1266:1281	arg1	model					1283:1287	Korsmeyer-Peppas model	1266:1287	Korsmeyer-Peppas model	1266:1287	For the release of curcumin in simulated intestinal fluid digestion, release mechanism explained by Korsmeyer-Peppas model.
33901554	5	59	theme	materials	1155:1163	arg1	content					1139:1145	higher content	1132:1145	higher content of host materials	1132:1163	UV, pH, and thermal stability of curcumin significantly enhanced by the encapsulation, which was further increased when using M.SNPs and/or higher content of host materials.
33901554	0	60	dep	Application	0:10	arg1	Effect					85:90	Effect	85:90	Application of starch nanoparticles as host materials for encapsulation of curcumin: Effect of citric acid modification.	0:119	Application of starch nanoparticles as host materials for encapsulation of curcumin: Effect of citric acid modification.
33901554	3	61	theme	forces	769:774	arg1	forces					769:774	the major driving forces	751:774	the major driving forces for encapsulation	751:792	The XRD results revealed that curcumin was amorphously encapsulated in the composite, and hydrogen bond formation between M.SNPs and curcumin was one of the major driving forces for encapsulation as suggested by FT-IR.
33901554	3	61	theme	forces	769:774	arg1	one					744:746	one	744:746	one	744:746	The XRD results revealed that curcumin was amorphously encapsulated in the composite, and hydrogen bond formation between M.SNPs and curcumin was one of the major driving forces for encapsulation as suggested by FT-IR.
33901554	3	62	theme	hydrogen	688:695	arg1	formation					702:710	hydrogen bond formation	688:710	hydrogen bond formation between M.SNPs and curcumin	688:738	The XRD results revealed that curcumin was amorphously encapsulated in the composite, and hydrogen bond formation between M.SNPs and curcumin was one of the major driving forces for encapsulation as suggested by FT-IR.
33256221	0	0	theme	Disease-Affected	69:84	arg1	Salmon					95:100	Healthy and Amebic Gill Disease-Affected Atlantic Salmon	45:100	Healthy and Amebic Gill Disease-Affected Atlantic Salmon	45:100	Gill Mucus and Gill Mucin O-glycosylation in Healthy and Amebic Gill Disease-Affected Atlantic Salmon.
33256221	9	1	gly	fucosylated	1177:1187	arg1	types					1212:1216	three types	1206:1216	of three types: Fuc-HexNAc-R, Gal-[Fuc-]HexNAc-R and HexNAc-[Fuc-]HexNAc-R	1203:1276	The fucosylated epitopes were of three types: Fuc-HexNAc-R, Gal-[Fuc-]HexNAc-R and HexNAc-[Fuc-]HexNAc-R.
33256221	9	1	gly	fucosylated	1177:1187	arg1	epitopes					1189:1196	The fucosylated epitopes	1173:1196	The fucosylated epitopes	1173:1196	The fucosylated epitopes were of three types: Fuc-HexNAc-R, Gal-[Fuc-]HexNAc-R and HexNAc-[Fuc-]HexNAc-R.
33256221	1	2	from	death	158:162	arg1	salmonids					167:175	salmonids	167:175	salmonids	167:175	Amoebic gill disease (AGD) causes poor performance and death in salmonids.
33256221	1	3	theme	poor	137:140	arg1	performance					142:152	poor performance	137:152	poor performance	137:152	Amoebic gill disease (AGD) causes poor performance and death in salmonids.
33256221	6	4	theme	Confocal	639:646	arg1	microscopy					648:657	Confocal microscopy	639:657	Confocal microscopy	639:657	Confocal microscopy revealed that fucosylated mucus coated sialylated mucus strands in ex vivo gill mucus.
33256221	7	5	theme	Terminal	746:753	arg1	HexNAcs					755:761	Terminal HexNAcs	746:761	Terminal HexNAcs	746:761	Terminal HexNAcs were more abundant among O-glycans from AGD-affected Atlantic salmon, whereas core 1 structures and structures with acidic moieties such as N-acetylneuraminic acid (NeuAc) and sulfate groups were less abundant compared to non-infected fish.
33256221	0	6	theme	Gill	64:67	arg1	Salmon					95:100	Healthy and Amebic Gill Disease-Affected Atlantic Salmon	45:100	Healthy and Amebic Gill Disease-Affected Atlantic Salmon	45:100	Gill Mucus and Gill Mucin O-glycosylation in Healthy and Amebic Gill Disease-Affected Atlantic Salmon.
33256221	4	7	theme	chromatography-mass	514:532	arg1	spectrometry					534:545	liquid chromatography-mass spectrometry	507:545	liquid chromatography-mass spectrometry	507:545	We investigated gill mucin O-glycosylation from Atlantic salmon with and without AGD using liquid chromatography-mass spectrometry.
33256221	7	8	theme	non-infected	985:996	arg1	fish					998:1001	non-infected fish	985:1001	non-infected fish	985:1001	Terminal HexNAcs were more abundant among O-glycans from AGD-affected Atlantic salmon, whereas core 1 structures and structures with acidic moieties such as N-acetylneuraminic acid (NeuAc) and sulfate groups were less abundant compared to non-infected fish.
33256221	7	9	with	structures	863:872	arg1	moieties					886:893	acidic moieties	879:893	acidic moieties	879:893	Terminal HexNAcs were more abundant among O-glycans from AGD-affected Atlantic salmon, whereas core 1 structures and structures with acidic moieties such as N-acetylneuraminic acid (NeuAc) and sulfate groups were less abundant compared to non-infected fish.
33256221	10	10	theme	blood	1285:1289	arg1	avenue					1325:1330	an avenue	1322:1330	an avenue to diversify the glycan repertoire to limit infection in the exposed gills	1322:1405	These blood group-like structures could be an avenue to diversify the glycan repertoire to limit infection in the exposed gills.
33256221	10	10	theme	blood	1285:1289	arg1	structures					1302:1311	These blood group-like structures	1279:1311	These blood group-like structures	1279:1311	These blood group-like structures could be an avenue to diversify the glycan repertoire to limit infection in the exposed gills.
33256221	3	11	theme	glycosylation	331:343	arg1	changes					345:351	glycosylation changes	331:351	glycosylation changes	331:351	Since glycans regulate pathogen binding and growth, glycosylation changes may affect susceptibility to primary and secondary infections.
33256221	4	12	theme	liquid	507:512	arg1	spectrometry					534:545	liquid chromatography-mass spectrometry	507:545	liquid chromatography-mass spectrometry	507:545	We investigated gill mucin O-glycosylation from Atlantic salmon with and without AGD using liquid chromatography-mass spectrometry.
33256221	8	13	theme	infected	1085:1092	arg1	fish					1094:1097	infected fish	1085:1097	infected fish on the lower scale of NeuAc abundance	1085:1135	The fucosylated and NeuAc-containing O-glycans were inversely proportional, with infected fish on the lower scale of NeuAc abundance and high on fucosylated structures.
33256221	1	14	from	performance	142:152	arg1	salmonids					167:175	salmonids	167:175	salmonids	167:175	Amoebic gill disease (AGD) causes poor performance and death in salmonids.
33256221	5	15	theme	mucin	553:557	arg1	glycans					559:565	Gill mucin glycans	548:565	Gill mucin glycans	548:565	Gill mucin glycans were larger and more complex, diverse and fucosylated than skin mucins.
33256221	0	16	theme	Atlantic	86:93	arg1	Salmon					95:100	Healthy and Amebic Gill Disease-Affected Atlantic Salmon	45:100	Healthy and Amebic Gill Disease-Affected Atlantic Salmon	45:100	Gill Mucus and Gill Mucin O-glycosylation in Healthy and Amebic Gill Disease-Affected Atlantic Salmon.
33256221	6	17	gly	fucosylated	673:683	arg1	mucus					685:689	fucosylated mucus	673:689	fucosylated mucus	673:689	Confocal microscopy revealed that fucosylated mucus coated sialylated mucus strands in ex vivo gill mucus.
33256221	11	18	theme	skin	1532:1535	arg1	mucins					1537:1542	skin mucins	1532:1542	skin mucins	1532:1542	Furthermore, care must be taken when using skin mucus as proxy for gill mucus, as gill mucins are distinctly different from skin mucins.
33256221	6	19	theme	gill	734:737	arg1	mucus					739:743	ex vivo gill mucus	726:743	ex vivo gill mucus	726:743	Confocal microscopy revealed that fucosylated mucus coated sialylated mucus strands in ex vivo gill mucus.
33256221	8	20	with	proportional	1066:1077	arg1	fish					1094:1097	infected fish	1085:1097	infected fish on the lower scale of NeuAc abundance	1085:1135	The fucosylated and NeuAc-containing O-glycans were inversely proportional, with infected fish on the lower scale of NeuAc abundance and high on fucosylated structures.
33256221	7	21	from	salmon	825:830	arg1	O-glycans					788:796	O-glycans	788:796	O-glycans from AGD-affected Atlantic salmon	788:830	Terminal HexNAcs were more abundant among O-glycans from AGD-affected Atlantic salmon, whereas core 1 structures and structures with acidic moieties such as N-acetylneuraminic acid (NeuAc) and sulfate groups were less abundant compared to non-infected fish.
33256221	5	22	theme	skin	626:629	arg1	mucins					631:636	skin mucins	626:636	skin mucins	626:636	Gill mucin glycans were larger and more complex, diverse and fucosylated than skin mucins.
33256221	2	23	theme	mucus	254:258	arg1	components					236:245	main components	231:245	main components of the mucus covering the gill	231:276	Mucins are mainly comprised by carbohydrates and are main components of the mucus covering the gill.
33256221	0	24	from	O-glycosylation	26:40	arg1	Salmon					95:100	Healthy and Amebic Gill Disease-Affected Atlantic Salmon	45:100	Healthy and Amebic Gill Disease-Affected Atlantic Salmon	45:100	Gill Mucus and Gill Mucin O-glycosylation in Healthy and Amebic Gill Disease-Affected Atlantic Salmon.
33256221	6	25	dep	ex	726:727	arg1	vivo					729:732	vivo	729:732	vivo	729:732	Confocal microscopy revealed that fucosylated mucus coated sialylated mucus strands in ex vivo gill mucus.
33256221	6	26	theme	ex	726:727	arg1	mucus					739:743	ex vivo gill mucus	726:743	ex vivo gill mucus	726:743	Confocal microscopy revealed that fucosylated mucus coated sialylated mucus strands in ex vivo gill mucus.
33256221	3	27	theme	pathogen	302:309	arg1	binding					311:317	pathogen binding	302:317	pathogen binding	302:317	Since glycans regulate pathogen binding and growth, glycosylation changes may affect susceptibility to primary and secondary infections.
33256221	8	28	theme	lower	1106:1110	arg1	scale					1112:1116	the lower scale	1102:1116	the lower scale of NeuAc abundance	1102:1135	The fucosylated and NeuAc-containing O-glycans were inversely proportional, with infected fish on the lower scale of NeuAc abundance and high on fucosylated structures.
33256221	8	29	theme	NeuAc-containing	1024:1039	arg1	O-glycans					1041:1049	The fucosylated and NeuAc-containing O-glycans	1004:1049	The fucosylated and NeuAc-containing O-glycans	1004:1049	The fucosylated and NeuAc-containing O-glycans were inversely proportional, with infected fish on the lower scale of NeuAc abundance and high on fucosylated structures.
33256221	8	29	theme	NeuAc-containing	1024:1039	arg1	proportional					1066:1077	proportional	1066:1077	proportional	1066:1077	The fucosylated and NeuAc-containing O-glycans were inversely proportional, with infected fish on the lower scale of NeuAc abundance and high on fucosylated structures.
33256221	7	30	theme	acidic	879:884	arg1	moieties					886:893	acidic moieties	879:893	acidic moieties	879:893	Terminal HexNAcs were more abundant among O-glycans from AGD-affected Atlantic salmon, whereas core 1 structures and structures with acidic moieties such as N-acetylneuraminic acid (NeuAc) and sulfate groups were less abundant compared to non-infected fish.
33256221	10	31	from	infection	1376:1384	arg1	gills					1401:1405	the exposed gills	1389:1405	the exposed gills	1389:1405	These blood group-like structures could be an avenue to diversify the glycan repertoire to limit infection in the exposed gills.
33256221	0	32	theme	Gill	0:3	arg1	Mucus					5:9	Gill Mucus	0:9	Gill Mucus	0:9	Gill Mucus and Gill Mucin O-glycosylation in Healthy and Amebic Gill Disease-Affected Atlantic Salmon.
33256221	4	33	from	salmon	473:478	arg1	O-glycosylation					443:457	gill mucin O-glycosylation	432:457	gill mucin O-glycosylation from Atlantic salmon with and without AGD	432:499	We investigated gill mucin O-glycosylation from Atlantic salmon with and without AGD using liquid chromatography-mass spectrometry.
33256221	10	34	theme	glycan	1349:1354	arg1	repertoire					1356:1365	the glycan repertoire	1345:1365	the glycan repertoire to limit infection in the exposed gills	1345:1405	These blood group-like structures could be an avenue to diversify the glycan repertoire to limit infection in the exposed gills.
33256221	7	35	with	structures	848:857	arg1	moieties					886:893	acidic moieties	879:893	acidic moieties	879:893	Terminal HexNAcs were more abundant among O-glycans from AGD-affected Atlantic salmon, whereas core 1 structures and structures with acidic moieties such as N-acetylneuraminic acid (NeuAc) and sulfate groups were less abundant compared to non-infected fish.
33256221	8	36	gly	fucosylated	1008:1018	arg1	O-glycans					1041:1049	The fucosylated and NeuAc-containing O-glycans	1004:1049	The fucosylated and NeuAc-containing O-glycans	1004:1049	The fucosylated and NeuAc-containing O-glycans were inversely proportional, with infected fish on the lower scale of NeuAc abundance and high on fucosylated structures.
33256221	8	36	gly	fucosylated	1008:1018	arg1	proportional					1066:1077	proportional	1066:1077	proportional	1066:1077	The fucosylated and NeuAc-containing O-glycans were inversely proportional, with infected fish on the lower scale of NeuAc abundance and high on fucosylated structures.
33256221	7	37	theme	core	841:844	arg1	acid					922:925	N-acetylneuraminic acid	903:925	N-acetylneuraminic acid (NeuAc)	903:933	Terminal HexNAcs were more abundant among O-glycans from AGD-affected Atlantic salmon, whereas core 1 structures and structures with acidic moieties such as N-acetylneuraminic acid (NeuAc) and sulfate groups were less abundant compared to non-infected fish.
33256221	7	37	theme	core	841:844	arg1	groups					947:952	sulfate groups	939:952	sulfate groups	939:952	Terminal HexNAcs were more abundant among O-glycans from AGD-affected Atlantic salmon, whereas core 1 structures and structures with acidic moieties such as N-acetylneuraminic acid (NeuAc) and sulfate groups were less abundant compared to non-infected fish.
33256221	7	37	theme	core	841:844	arg1	structures					848:857	core 1 structures	841:857	core 1 structures	841:857	Terminal HexNAcs were more abundant among O-glycans from AGD-affected Atlantic salmon, whereas core 1 structures and structures with acidic moieties such as N-acetylneuraminic acid (NeuAc) and sulfate groups were less abundant compared to non-infected fish.
33256221	8	38	theme	fucosylated	1008:1018	arg1	O-glycans					1041:1049	The fucosylated and NeuAc-containing O-glycans	1004:1049	The fucosylated and NeuAc-containing O-glycans	1004:1049	The fucosylated and NeuAc-containing O-glycans were inversely proportional, with infected fish on the lower scale of NeuAc abundance and high on fucosylated structures.
33256221	8	38	theme	fucosylated	1008:1018	arg1	proportional					1066:1077	proportional	1066:1077	proportional	1066:1077	The fucosylated and NeuAc-containing O-glycans were inversely proportional, with infected fish on the lower scale of NeuAc abundance and high on fucosylated structures.
33256221	6	39	from	strands	715:721	arg1	mucus					739:743	ex vivo gill mucus	726:743	ex vivo gill mucus	726:743	Confocal microscopy revealed that fucosylated mucus coated sialylated mucus strands in ex vivo gill mucus.
33256221	6	40	theme	mucus	709:713	arg1	strands					715:721	sialylated mucus strands	698:721	sialylated mucus strands in ex vivo gill mucus	698:743	Confocal microscopy revealed that fucosylated mucus coated sialylated mucus strands in ex vivo gill mucus.
33256221	1	41	theme	Amoebic	103:109	arg1	AGD					125:127	AGD	125:127	AGD	125:127	Amoebic gill disease (AGD) causes poor performance and death in salmonids.
33256221	1	41	theme	Amoebic	103:109	arg1	disease					116:122	Amoebic gill disease	103:122	Amoebic gill disease (AGD)	103:128	Amoebic gill disease (AGD) causes poor performance and death in salmonids.
33256221	6	42	theme	sialylated	698:707	arg1	strands					715:721	sialylated mucus strands	698:721	sialylated mucus strands in ex vivo gill mucus	698:743	Confocal microscopy revealed that fucosylated mucus coated sialylated mucus strands in ex vivo gill mucus.
33256221	11	43	theme	gill	1475:1478	arg1	mucus					1480:1484	gill mucus	1475:1484	gill mucus	1475:1484	Furthermore, care must be taken when using skin mucus as proxy for gill mucus, as gill mucins are distinctly different from skin mucins.
33256221	3	44	theme	secondary	394:402	arg1	infections					404:413	primary and secondary infections	382:413	primary and secondary infections	382:413	Since glycans regulate pathogen binding and growth, glycosylation changes may affect susceptibility to primary and secondary infections.
33256221	8	45	theme	abundance	1127:1135	arg1	scale					1112:1116	the lower scale	1102:1116	the lower scale of NeuAc abundance	1102:1135	The fucosylated and NeuAc-containing O-glycans were inversely proportional, with infected fish on the lower scale of NeuAc abundance and high on fucosylated structures.
33256221	4	46	theme	mucin	437:441	arg1	O-glycosylation					443:457	gill mucin O-glycosylation	432:457	gill mucin O-glycosylation from Atlantic salmon with and without AGD	432:499	We investigated gill mucin O-glycosylation from Atlantic salmon with and without AGD using liquid chromatography-mass spectrometry.
33256221	8	47	gly	fucosylated	1149:1159	arg1	structures					1161:1170	fucosylated structures	1149:1170	fucosylated structures	1149:1170	The fucosylated and NeuAc-containing O-glycans were inversely proportional, with infected fish on the lower scale of NeuAc abundance and high on fucosylated structures.
33256221	7	48	theme	AGD-affected	803:814	arg1	salmon					825:830	AGD-affected Atlantic salmon	803:830	AGD-affected Atlantic salmon	803:830	Terminal HexNAcs were more abundant among O-glycans from AGD-affected Atlantic salmon, whereas core 1 structures and structures with acidic moieties such as N-acetylneuraminic acid (NeuAc) and sulfate groups were less abundant compared to non-infected fish.
33256221	8	49	theme	NeuAc	1121:1125	arg1	abundance					1127:1135	NeuAc abundance	1121:1135	NeuAc abundance	1121:1135	The fucosylated and NeuAc-containing O-glycans were inversely proportional, with infected fish on the lower scale of NeuAc abundance and high on fucosylated structures.
33256221	4	50	theme	Atlantic	464:471	arg1	salmon					473:478	Atlantic salmon	464:478	Atlantic salmon	464:478	We investigated gill mucin O-glycosylation from Atlantic salmon with and without AGD using liquid chromatography-mass spectrometry.
33256221	3	51	theme	primary	382:388	arg1	infections					404:413	primary and secondary infections	382:413	primary and secondary infections	382:413	Since glycans regulate pathogen binding and growth, glycosylation changes may affect susceptibility to primary and secondary infections.
33256221	7	52	theme	Atlantic	816:823	arg1	salmon					825:830	AGD-affected Atlantic salmon	803:830	AGD-affected Atlantic salmon	803:830	Terminal HexNAcs were more abundant among O-glycans from AGD-affected Atlantic salmon, whereas core 1 structures and structures with acidic moieties such as N-acetylneuraminic acid (NeuAc) and sulfate groups were less abundant compared to non-infected fish.
33256221	6	53	gly	sialylated	698:707	arg1	strands					715:721	sialylated mucus strands	698:721	sialylated mucus strands in ex vivo gill mucus	698:743	Confocal microscopy revealed that fucosylated mucus coated sialylated mucus strands in ex vivo gill mucus.
33256221	1	54	theme	gill	111:114	arg1	AGD					125:127	AGD	125:127	AGD	125:127	Amoebic gill disease (AGD) causes poor performance and death in salmonids.
33256221	1	54	theme	gill	111:114	arg1	disease					116:122	Amoebic gill disease	103:122	Amoebic gill disease (AGD)	103:128	Amoebic gill disease (AGD) causes poor performance and death in salmonids.
33256221	11	55	theme	skin	1451:1454	arg1	mucus					1456:1460	skin mucus	1451:1460	skin mucus	1451:1460	Furthermore, care must be taken when using skin mucus as proxy for gill mucus, as gill mucins are distinctly different from skin mucins.
33256221	7	56	dep	abundant	773:780	arg1	whereas					833:839	whereas	833:839	whereas	833:839	Terminal HexNAcs were more abundant among O-glycans from AGD-affected Atlantic salmon, whereas core 1 structures and structures with acidic moieties such as N-acetylneuraminic acid (NeuAc) and sulfate groups were less abundant compared to non-infected fish.
33256221	11	57	theme	gill	1490:1493	arg1	mucins					1495:1500	gill mucins	1490:1500	gill mucins	1490:1500	Furthermore, care must be taken when using skin mucus as proxy for gill mucus, as gill mucins are distinctly different from skin mucins.
33256221	8	58	from	fish	1094:1097	arg1	scale					1112:1116	the lower scale	1102:1116	the lower scale of NeuAc abundance	1102:1135	The fucosylated and NeuAc-containing O-glycans were inversely proportional, with infected fish on the lower scale of NeuAc abundance and high on fucosylated structures.
33256221	8	59	theme	fucosylated	1149:1159	arg1	structures					1161:1170	fucosylated structures	1149:1170	fucosylated structures	1149:1170	The fucosylated and NeuAc-containing O-glycans were inversely proportional, with infected fish on the lower scale of NeuAc abundance and high on fucosylated structures.
33256221	9	60	theme	fucosylated	1177:1187	arg1	types					1212:1216	three types	1206:1216	of three types: Fuc-HexNAc-R, Gal-[Fuc-]HexNAc-R and HexNAc-[Fuc-]HexNAc-R	1203:1276	The fucosylated epitopes were of three types: Fuc-HexNAc-R, Gal-[Fuc-]HexNAc-R and HexNAc-[Fuc-]HexNAc-R.
33256221	9	60	theme	fucosylated	1177:1187	arg1	epitopes					1189:1196	The fucosylated epitopes	1173:1196	The fucosylated epitopes	1173:1196	The fucosylated epitopes were of three types: Fuc-HexNAc-R, Gal-[Fuc-]HexNAc-R and HexNAc-[Fuc-]HexNAc-R.
33256221	6	61	theme	fucosylated	673:683	arg1	mucus					685:689	fucosylated mucus	673:689	fucosylated mucus	673:689	Confocal microscopy revealed that fucosylated mucus coated sialylated mucus strands in ex vivo gill mucus.
33256221	9	62	dep	HexNAc-R	1269:1276	arg1	Gal-[Fuc-					1233:1241	Gal-[Fuc-	1233:1241	Gal-[Fuc-	1233:1241	The fucosylated epitopes were of three types: Fuc-HexNAc-R, Gal-[Fuc-]HexNAc-R and HexNAc-[Fuc-]HexNAc-R.
33256221	4	63	theme	gill	432:435	arg1	O-glycosylation					443:457	gill mucin O-glycosylation	432:457	gill mucin O-glycosylation from Atlantic salmon with and without AGD	432:499	We investigated gill mucin O-glycosylation from Atlantic salmon with and without AGD using liquid chromatography-mass spectrometry.
33256221	0	64	gly	O-glycosylation	26:40	arg1	Salmon					95:100	Healthy and Amebic Gill Disease-Affected Atlantic Salmon	45:100	Healthy and Amebic Gill Disease-Affected Atlantic Salmon	45:100	Gill Mucus and Gill Mucin O-glycosylation in Healthy and Amebic Gill Disease-Affected Atlantic Salmon.
33256221	9	65	dep	types	1212:1216	arg1	Fuc-HexNAc-R					1219:1230	Fuc-HexNAc-R	1219:1230	Fuc-HexNAc-R	1219:1230	The fucosylated epitopes were of three types: Fuc-HexNAc-R, Gal-[Fuc-]HexNAc-R and HexNAc-[Fuc-]HexNAc-R.
33256221	9	65	dep	types	1212:1216	arg1	HexNAc-R					1269:1276	HexNAc-R	1269:1276	HexNAc-R	1269:1276	The fucosylated epitopes were of three types: Fuc-HexNAc-R, Gal-[Fuc-]HexNAc-R and HexNAc-[Fuc-]HexNAc-R.
33256221	9	65	dep	types	1212:1216	arg1	HexNAc-[Fuc-					1256:1267	HexNAc-[Fuc-	1256:1267	HexNAc-[Fuc-	1256:1267	The fucosylated epitopes were of three types: Fuc-HexNAc-R, Gal-[Fuc-]HexNAc-R and HexNAc-[Fuc-]HexNAc-R.
33256221	9	65	dep	types	1212:1216	arg1	HexNAc-R					1243:1250	HexNAc-R	1243:1250	HexNAc-R	1243:1250	The fucosylated epitopes were of three types: Fuc-HexNAc-R, Gal-[Fuc-]HexNAc-R and HexNAc-[Fuc-]HexNAc-R.
33256221	0	66	theme	Amebic	57:62	arg1	Salmon					95:100	Healthy and Amebic Gill Disease-Affected Atlantic Salmon	45:100	Healthy and Amebic Gill Disease-Affected Atlantic Salmon	45:100	Gill Mucus and Gill Mucin O-glycosylation in Healthy and Amebic Gill Disease-Affected Atlantic Salmon.
33256221	7	67	theme	N-acetylneuraminic	903:920	arg1	acid					922:925	N-acetylneuraminic acid	903:925	N-acetylneuraminic acid (NeuAc)	903:933	Terminal HexNAcs were more abundant among O-glycans from AGD-affected Atlantic salmon, whereas core 1 structures and structures with acidic moieties such as N-acetylneuraminic acid (NeuAc) and sulfate groups were less abundant compared to non-infected fish.
33256221	7	67	theme	N-acetylneuraminic	903:920	arg1	NeuAc					928:932	NeuAc	928:932	NeuAc	928:932	Terminal HexNAcs were more abundant among O-glycans from AGD-affected Atlantic salmon, whereas core 1 structures and structures with acidic moieties such as N-acetylneuraminic acid (NeuAc) and sulfate groups were less abundant compared to non-infected fish.
33256221	2	68	theme	main	231:234	arg1	components					236:245	main components	231:245	main components of the mucus covering the gill	231:276	Mucins are mainly comprised by carbohydrates and are main components of the mucus covering the gill.
33256221	10	69	theme	exposed	1393:1399	arg1	gills					1401:1405	the exposed gills	1389:1405	the exposed gills	1389:1405	These blood group-like structures could be an avenue to diversify the glycan repertoire to limit infection in the exposed gills.
33256221	7	70	theme	sulfate	939:945	arg1	groups					947:952	sulfate groups	939:952	sulfate groups	939:952	Terminal HexNAcs were more abundant among O-glycans from AGD-affected Atlantic salmon, whereas core 1 structures and structures with acidic moieties such as N-acetylneuraminic acid (NeuAc) and sulfate groups were less abundant compared to non-infected fish.
33256221	0	71	theme	Healthy	45:51	arg1	Salmon					95:100	Healthy and Amebic Gill Disease-Affected Atlantic Salmon	45:100	Healthy and Amebic Gill Disease-Affected Atlantic Salmon	45:100	Gill Mucus and Gill Mucin O-glycosylation in Healthy and Amebic Gill Disease-Affected Atlantic Salmon.
33256221	5	72	theme	Gill	548:551	arg1	glycans					559:565	Gill mucin glycans	548:565	Gill mucin glycans	548:565	Gill mucin glycans were larger and more complex, diverse and fucosylated than skin mucins.
33256221	11	73	from	mucins	1537:1542	arg1	different					1517:1525	different	1517:1525	different	1517:1525	Furthermore, care must be taken when using skin mucus as proxy for gill mucus, as gill mucins are distinctly different from skin mucins.
33256221	10	74	theme	group-like	1291:1300	arg1	avenue					1325:1330	an avenue	1322:1330	an avenue to diversify the glycan repertoire to limit infection in the exposed gills	1322:1405	These blood group-like structures could be an avenue to diversify the glycan repertoire to limit infection in the exposed gills.
33256221	10	74	theme	group-like	1291:1300	arg1	structures					1302:1311	These blood group-like structures	1279:1311	These blood group-like structures	1279:1311	These blood group-like structures could be an avenue to diversify the glycan repertoire to limit infection in the exposed gills.
33256221	9	75	theme	of	1203:1204	arg1	types					1212:1216	three types	1206:1216	of three types: Fuc-HexNAc-R, Gal-[Fuc-]HexNAc-R and HexNAc-[Fuc-]HexNAc-R	1203:1276	The fucosylated epitopes were of three types: Fuc-HexNAc-R, Gal-[Fuc-]HexNAc-R and HexNAc-[Fuc-]HexNAc-R.
33256221	9	75	theme	of	1203:1204	arg1	epitopes					1189:1196	The fucosylated epitopes	1173:1196	The fucosylated epitopes	1173:1196	The fucosylated epitopes were of three types: Fuc-HexNAc-R, Gal-[Fuc-]HexNAc-R and HexNAc-[Fuc-]HexNAc-R.
32122481	1	0	theme	Zeta	664:667	arg1	Potential					669:677	Zeta Potential	664:677	Zeta Potential	664:677	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	0	1	from	characterization	14:29	arg1	Sagittaria					103:112	Sagittaria	103:112	Sagittaria	103:112	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	5	2	from	use	1211:1213	arg1	medicine					1232:1239	complementary medicine	1218:1239	complementary medicine	1218:1239	SSP has greater potential to be explored as biologicalagents for use in complementary medicine or functional foods.
32122481	5	2	from	use	1211:1213	arg1	foods					1255:1259	functional foods	1244:1259	functional foods	1244:1259	SSP has greater potential to be explored as biologicalagents for use in complementary medicine or functional foods.
32122481	1	3	theme	Infrared	605:612	arg1	IR					628:629	IR	628:629	IR	628:629	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	1	3	theme	Infrared	605:612	arg1	Spectroscopy					614:625	Infrared Spectroscopy	605:625	Infrared Spectroscopy (IR)	605:630	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	3	4	theme	Molecular	798:806	arg1	weight					808:813	Molecular weight	798:813	Molecular weight	798:813	Molecular weight and monosaccharide composition analysis exhibited that SSW (2275.0 kDa), SSU (148.7 kDa), and SSP (1984.0 kDa) were heteropolysaccharide with dramatically different monosaccharide species and mole ratios.
32122481	0	5	theme	polysaccharides	82:96	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	5	theme	polysaccharides	82:96	arg1	antioxidant					32:42	antioxidant	32:42	antioxidant	32:42	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	5	theme	polysaccharides	82:96	arg1	activities					62:71	immunological activities	48:71	immunological activities	48:71	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	6	theme	biological	204:213	arg1	activity					215:222	biological activity	204:222	biological activity	204:222	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	3	7	theme	monosaccharide	980:993	arg1	species					995:1001	dramatically different monosaccharide species	957:1001	dramatically different monosaccharide species	957:1001	Molecular weight and monosaccharide composition analysis exhibited that SSW (2275.0 kDa), SSU (148.7 kDa), and SSP (1984.0 kDa) were heteropolysaccharide with dramatically different monosaccharide species and mole ratios.
32122481	0	8	theme	water	431:435	arg1	extraction					437:446	subcritical water extraction	419:446	subcritical water extraction	419:446	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	9	from	characteristics	184:198	arg1	vitro					227:231	vitro	227:231	vitro	227:231	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	9	from	characteristics	184:198	arg1	L					281:281	Sagittaria sagittifolia L	257:281	Sagittaria sagittifolia L. (SSs)	257:288	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	9	from	characteristics	184:198	arg1	SSs					285:287	SSs	285:287	SSs	285:287	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	10	theme	subcritical	419:429	arg1	extraction					437:446	subcritical water extraction	419:446	subcritical water extraction	419:446	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	1	11	theme	Congo	683:687	arg1	methods					693:699	Congo red methods	683:699	Congo red methods	683:699	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	0	12	from	activities	62:71	arg1	Sagittaria					103:112	Sagittaria	103:112	Sagittaria	103:112	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	1	13	theme	Performance	502:512	arg1	HPLC					537:540	HPLC	537:540	HPLC	537:540	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	1	13	theme	Performance	502:512	arg1	Chromatography					521:534	High Performance Liquid Chromatography	497:534	High Performance Liquid Chromatography (HPLC)	497:541	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	1	14	theme	Gas	544:546	arg1	GC					564:565	GC	564:565	GC	564:565	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	1	14	theme	Gas	544:546	arg1	Chromatography					548:561	Gas Chromatography	544:561	Gas Chromatography (GC)	544:566	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	1	15	theme	red	689:691	arg1	methods					693:699	Congo red methods	683:699	Congo red methods	683:699	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	4	16	theme	immunomodulatory	1102:1117	arg1	activity					1119:1126	more potent immunomodulatory activity	1090:1126	more potent immunomodulatory activity	1090:1126	In addition, SSP exhibited stronger antioxidant activity in vitro and more potent immunomodulatory activity than SSW and SSU.
32122481	0	17	theme	polysaccharides	236:250	arg1	characteristics					184:198	preliminary structural characteristics	161:198	preliminary structural characteristics	161:198	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	17	theme	polysaccharides	236:250	arg1	activity					215:222	biological activity	204:222	biological activity	204:222	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	1	18	theme	structural	455:464	arg1	features					466:473	Their structural features	449:473	Their structural features	449:473	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	4	19	theme	potent	1095:1100	arg1	activity					1119:1126	more potent immunomodulatory activity	1090:1126	more potent immunomodulatory activity	1090:1126	In addition, SSP exhibited stronger antioxidant activity in vitro and more potent immunomodulatory activity than SSW and SSU.
32122481	0	20	dep	Sagittaria	257:266	arg1	sagittifolia					268:279	sagittifolia	268:279	sagittifolia	268:279	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	21	dep	Sagittaria	103:112	arg1	L.					127:128	Sagittaria sagittifolia L.	103:128	Sagittaria sagittifolia L.	103:128	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	22	from	antioxidant	32:42	arg1	Sagittaria					103:112	Sagittaria	103:112	Sagittaria	103:112	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	23	theme	Sagittaria	257:266	arg1	L					281:281	Sagittaria sagittifolia L	257:281	Sagittaria sagittifolia L. (SSs)	257:288	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	23	theme	Sagittaria	257:266	arg1	SSs					285:287	SSs	285:287	SSs	285:287	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	4	24	theme	stronger	1047:1054	arg1	activity					1068:1075	stronger antioxidant activity	1047:1075	stronger antioxidant activity in vitro	1047:1084	In addition, SSP exhibited stronger antioxidant activity in vitro and more potent immunomodulatory activity than SSW and SSU.
32122481	0	25	theme	different	293:301	arg1	methods					313:319	different extration methods	293:319	different extration methods	293:319	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	2	26	theme	immunostimulatory	744:760	arg1	effects					762:768	immunostimulatory effects	744:768	immunostimulatory effects	744:768	Furthermore, the antioxidant activity and immunostimulatory effects were investigated in vitro.
32122481	4	27	theme	antioxidant	1056:1066	arg1	activity					1068:1075	stronger antioxidant activity	1047:1075	stronger antioxidant activity in vitro	1047:1084	In addition, SSP exhibited stronger antioxidant activity in vitro and more potent immunomodulatory activity than SSW and SSU.
32122481	3	28	theme	different	970:978	arg1	species					995:1001	dramatically different monosaccharide species	957:1001	dramatically different monosaccharide species	957:1001	Molecular weight and monosaccharide composition analysis exhibited that SSW (2275.0 kDa), SSU (148.7 kDa), and SSP (1984.0 kDa) were heteropolysaccharide with dramatically different monosaccharide species and mole ratios.
32122481	0	29	theme	characterization	14:29	arg1	Comparison					0:9	Comparison	0:9	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L.	0:128	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	30	from	activity	215:222	arg1	vitro					227:231	vitro	227:231	vitro	227:231	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	30	from	activity	215:222	arg1	L					281:281	Sagittaria sagittifolia L	257:281	Sagittaria sagittifolia L. (SSs)	257:288	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	30	from	activity	215:222	arg1	SSs					285:287	SSs	285:287	SSs	285:287	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	5	31	theme	greater	1154:1160	arg1	potential					1162:1170	greater potential	1154:1170	greater potential	1154:1170	SSP has greater potential to be explored as biologicalagents for use in complementary medicine or functional foods.
32122481	2	32	theme	antioxidant	719:729	arg1	activity					731:738	the antioxidant activity	715:738	the antioxidant activity	715:738	Furthermore, the antioxidant activity and immunostimulatory effects were investigated in vitro.
32122481	1	33	theme	Scanning	569:576	arg1	SEM					599:601	SEM	599:601	SEM	599:601	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	1	33	theme	Scanning	569:576	arg1	Microscopy					587:596	Scanning Electron Microscopy	569:596	Scanning Electron Microscopy (SEM)	569:602	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	1	34	theme	Force	640:644	arg1	Microscopy					646:655	Atomic Force Microscopy	633:655	Atomic Force Microscopy (AFM)	633:661	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	1	34	theme	Force	640:644	arg1	AFM					658:660	AFM	658:660	AFM	658:660	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	0	35	dep	polysaccharides	328:342	arg1	SSU					350:352	SSU	350:352	SSU	350:352	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	35	dep	polysaccharides	328:342	arg1	SSP					359:361	SSP	359:361	SSP	359:361	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	35	dep	polysaccharides	328:342	arg1	SSW					345:347	SSW	345:347	SSW	345:347	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	35	dep	polysaccharides	328:342	arg1	polysaccharides					328:342	three polysaccharides	322:342	three polysaccharides (SSW, SSU, and SSP)	322:362	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	5	36	contain	has	1150:1152	arg2	potential					1162:1170	greater potential	1154:1170	greater potential	1154:1170	SSP has greater potential to be explored as biologicalagents for use in complementary medicine or functional foods.
32122481	5	36	contain	has	1150:1152	arg1	SSP					1146:1148	SSP	1146:1148	SSP	1146:1148	SSP has greater potential to be explored as biologicalagents for use in complementary medicine or functional foods.
32122481	1	37	theme	Electron	578:585	arg1	SEM					599:601	SEM	599:601	SEM	599:601	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	1	37	theme	Electron	578:585	arg1	Microscopy					587:596	Scanning Electron Microscopy	569:596	Scanning Electron Microscopy (SEM)	569:602	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	0	38	theme	antioxidant	32:42	arg1	Comparison					0:9	Comparison	0:9	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L.	0:128	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	3	39	theme	weight	808:813	arg1	analysis					846:853	Molecular weight and monosaccharide composition analysis	798:853	Molecular weight and monosaccharide composition analysis	798:853	Molecular weight and monosaccharide composition analysis exhibited that SSW (2275.0 kDa), SSU (148.7 kDa), and SSP (1984.0 kDa) were heteropolysaccharide with dramatically different monosaccharide species and mole ratios.
32122481	1	40	theme	High	497:500	arg1	HPLC					537:540	HPLC	537:540	HPLC	537:540	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	1	40	theme	High	497:500	arg1	Chromatography					521:534	High Performance Liquid Chromatography	497:534	High Performance Liquid Chromatography (HPLC)	497:541	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	0	41	theme	activities	62:71	arg1	Comparison					0:9	Comparison	0:9	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L.	0:128	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	5	42	theme	functional	1244:1253	arg1	foods					1255:1259	functional foods	1244:1259	functional foods	1244:1259	SSP has greater potential to be explored as biologicalagents for use in complementary medicine or functional foods.
32122481	0	43	theme	structural	173:182	arg1	characteristics					184:198	preliminary structural characteristics	161:198	preliminary structural characteristics	161:198	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	44	theme	extration	303:311	arg1	methods					313:319	different extration methods	293:319	different extration methods	293:319	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	45	theme	immunological	48:60	arg1	activities					62:71	immunological activities	48:71	immunological activities	48:71	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	46	theme	preliminary	161:171	arg1	characteristics					184:198	preliminary structural characteristics	161:198	preliminary structural characteristics	161:198	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	1	47	theme	Liquid	514:519	arg1	HPLC					537:540	HPLC	537:540	HPLC	537:540	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	1	47	theme	Liquid	514:519	arg1	Chromatography					521:534	High Performance Liquid Chromatography	497:534	High Performance Liquid Chromatography (HPLC)	497:541	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	0	48	dep	characteristics	184:198	arg1	the					157:159	the	157:159	the	157:159	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	3	49	theme	composition	834:844	arg1	analysis					846:853	Molecular weight and monosaccharide composition analysis	798:853	Molecular weight and monosaccharide composition analysis	798:853	Molecular weight and monosaccharide composition analysis exhibited that SSW (2275.0 kDa), SSU (148.7 kDa), and SSP (1984.0 kDa) were heteropolysaccharide with dramatically different monosaccharide species and mole ratios.
32122481	3	50	theme	mole	1007:1010	arg1	ratios					1012:1017	mole ratios	1007:1017	mole ratios	1007:1017	Molecular weight and monosaccharide composition analysis exhibited that SSW (2275.0 kDa), SSU (148.7 kDa), and SSP (1984.0 kDa) were heteropolysaccharide with dramatically different monosaccharide species and mole ratios.
32122481	0	51	from	L	281:281	arg1	polysaccharides					236:250	polysaccharides	236:250	polysaccharides from Sagittaria sagittifolia L. (SSs)	236:288	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	51	from	L	281:281	arg1	characteristics					184:198	preliminary structural characteristics	161:198	preliminary structural characteristics	161:198	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	51	from	L	281:281	arg1	activity					215:222	biological activity	204:222	biological activity	204:222	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	52	theme	hot	383:385	arg1	water					387:391	hot water	383:391	hot water	383:391	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	53	from	Sagittaria	103:112	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	53	from	Sagittaria	103:112	arg1	polysaccharides					82:96	three polysaccharides	76:96	three polysaccharides from Sagittaria sagittifolia L.	76:128	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	53	from	Sagittaria	103:112	arg1	antioxidant					32:42	antioxidant	32:42	antioxidant	32:42	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	0	53	from	Sagittaria	103:112	arg1	activities					62:71	immunological activities	48:71	immunological activities	48:71	Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L. To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction.
32122481	3	54	theme	monosaccharide	819:832	arg1	composition					834:844	monosaccharide composition	819:844	monosaccharide composition	819:844	Molecular weight and monosaccharide composition analysis exhibited that SSW (2275.0 kDa), SSU (148.7 kDa), and SSP (1984.0 kDa) were heteropolysaccharide with dramatically different monosaccharide species and mole ratios.
32122481	3	55	with	heteropolysaccharide	931:950	arg1	species					995:1001	dramatically different monosaccharide species	957:1001	dramatically different monosaccharide species	957:1001	Molecular weight and monosaccharide composition analysis exhibited that SSW (2275.0 kDa), SSU (148.7 kDa), and SSP (1984.0 kDa) were heteropolysaccharide with dramatically different monosaccharide species and mole ratios.
32122481	3	55	with	heteropolysaccharide	931:950	arg1	ratios					1012:1017	mole ratios	1007:1017	mole ratios	1007:1017	Molecular weight and monosaccharide composition analysis exhibited that SSW (2275.0 kDa), SSU (148.7 kDa), and SSP (1984.0 kDa) were heteropolysaccharide with dramatically different monosaccharide species and mole ratios.
32122481	1	56	theme	Atomic	633:638	arg1	Microscopy					646:655	Atomic Force Microscopy	633:655	Atomic Force Microscopy (AFM)	633:661	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	1	56	theme	Atomic	633:638	arg1	AFM					658:660	AFM	658:660	AFM	658:660	Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods.
32122481	5	57	theme	complementary	1218:1230	arg1	medicine					1232:1239	complementary medicine	1218:1239	complementary medicine	1218:1239	SSP has greater potential to be explored as biologicalagents for use in complementary medicine or functional foods.
32781121	0	0	theme	AAP-3-1	85:91	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Purification, characterization and in vitro antioxidant activity of a polysaccharide AAP-3-1 from Auricularia auricula.
32781121	0	0	theme	AAP-3-1	85:91	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, characterization and in vitro antioxidant activity of a polysaccharide AAP-3-1 from Auricularia auricula.
32781121	0	0	theme	AAP-3-1	85:91	arg1	activity					56:63	in vitro antioxidant activity	35:63	in vitro antioxidant activity	35:63	Purification, characterization and in vitro antioxidant activity of a polysaccharide AAP-3-1 from Auricularia auricula.
32781121	6	1	theme	concentration-dependent	1001:1023	arg1	manner					1025:1030	a concentration-dependent manner	999:1030	a concentration-dependent manner	999:1030	AAP-3-1 exhibited antioxidant activity in a concentration-dependent manner and the scavenging rates at 1.6 mg/mL on superoxide anions and hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals were 88.13%, 93.03% and 68.31%, respectively.
32781121	7	2	theme	oxidative	1270:1278	arg1	stress					1280:1285	2,2'-azobis-2-methyl-propanimidamide-induced oxidative stress	1225:1285	2,2'-azobis-2-methyl-propanimidamide-induced oxidative stress in HepG2 cells	1225:1300	AAP-3-1 effectively ameliorated 2,2'-azobis-2-methyl-propanimidamide-induced oxidative stress in HepG2 cells by inhibiting reactive oxygen species generation, decreasing the content of malondialdehyde, and increasing the activities of superoxide dismutase, glutathione peroxidase and catalase.
32781121	1	3	from	activity	178:185	arg1	auricula					238:245	Auricularia auricula	226:245	Auricularia auricula	226:245	The objective was to investigate the in vitro antioxidant activity of a novel polysaccharide AAP-3-1 from Auricularia auricula.
32781121	1	4	theme	in	157:158	arg1	activity					178:185	the in vitro antioxidant activity	153:185	the in vitro antioxidant activity of a novel polysaccharide AAP-3-1 from Auricularia auricula	153:245	The objective was to investigate the in vitro antioxidant activity of a novel polysaccharide AAP-3-1 from Auricularia auricula.
32781121	4	5	theme	Ultraviolet	639:649	arg1	spectroscopy					659:670	Ultraviolet visible spectroscopy	639:670	Ultraviolet visible spectroscopy	639:670	Ultraviolet visible spectroscopy, Fourier transform infrared spectroscopy and scanning electron microscopy were used for structural characterization.
32781121	0	6	theme	polysaccharide	70:83	arg1	AAP-3-1					85:91	a polysaccharide AAP-3-1	68:91	a polysaccharide AAP-3-1	68:91	Purification, characterization and in vitro antioxidant activity of a polysaccharide AAP-3-1 from Auricularia auricula.
32781121	5	7	from	mannose	873:879	arg1	ratio					904:908	a molar ratio	896:908	a molar ratio of 1.4:1 with a molecular weight of 320.9 kDa	896:954	The results showed that AAP-3-1 is a heteropolysaccharide and is mainly composed of mannose and glucose in a molar ratio of 1.4:1 with a molecular weight of 320.9 kDa.
32781121	3	8	theme	high	534:537	arg1	chromatography					566:579	high performance gel permeation chromatography	534:579	high performance gel permeation chromatography	534:579	The molecular weight and monosaccharide composition were determined by high performance gel permeation chromatography and high-performance liquid chromatography, respectively.
32781121	6	9	theme	hydroxyl	1095:1102	arg1	radicals					1138:1145	hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals	1095:1145	radicals	1138:1145	AAP-3-1 exhibited antioxidant activity in a concentration-dependent manner and the scavenging rates at 1.6 mg/mL on superoxide anions and hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals were 88.13%, 93.03% and 68.31%, respectively.
32781121	7	10	theme	catalase	1477:1484	arg1	activities					1414:1423	the activities	1410:1423	the activities of superoxide dismutase, glutathione peroxidase and catalase	1410:1484	AAP-3-1 effectively ameliorated 2,2'-azobis-2-methyl-propanimidamide-induced oxidative stress in HepG2 cells by inhibiting reactive oxygen species generation, decreasing the content of malondialdehyde, and increasing the activities of superoxide dismutase, glutathione peroxidase and catalase.
32781121	1	11	dep	in	157:158	arg1	vitro					160:164	vitro	160:164	vitro	160:164	The objective was to investigate the in vitro antioxidant activity of a novel polysaccharide AAP-3-1 from Auricularia auricula.
32781121	4	12	dep	transform	681:689	arg1	infrared					691:698	infrared	691:698	transform infrared spectroscopy	681:711	Ultraviolet visible spectroscopy, Fourier transform infrared spectroscopy and scanning electron microscopy were used for structural characterization.
32781121	5	13	with	ratio	904:908	arg1	weight					936:941	a molecular weight	924:941	a molecular weight of 320.9 kDa	924:954	The results showed that AAP-3-1 is a heteropolysaccharide and is mainly composed of mannose and glucose in a molar ratio of 1.4:1 with a molecular weight of 320.9 kDa.
32781121	3	14	theme	high-performance	585:600	arg1	chromatography					609:622	high-performance liquid chromatography	585:622	high-performance liquid chromatography	585:622	The molecular weight and monosaccharide composition were determined by high performance gel permeation chromatography and high-performance liquid chromatography, respectively.
32781121	4	15	theme	visible	651:657	arg1	spectroscopy					659:670	Ultraviolet visible spectroscopy	639:670	Ultraviolet visible spectroscopy	639:670	Ultraviolet visible spectroscopy, Fourier transform infrared spectroscopy and scanning electron microscopy were used for structural characterization.
32781121	7	16	theme	2,2'-azobis-2-methyl-propanimidamide-induced	1225:1268	arg1	stress					1280:1285	2,2'-azobis-2-methyl-propanimidamide-induced oxidative stress	1225:1285	2,2'-azobis-2-methyl-propanimidamide-induced oxidative stress in HepG2 cells	1225:1300	AAP-3-1 effectively ameliorated 2,2'-azobis-2-methyl-propanimidamide-induced oxidative stress in HepG2 cells by inhibiting reactive oxygen species generation, decreasing the content of malondialdehyde, and increasing the activities of superoxide dismutase, glutathione peroxidase and catalase.
32781121	6	17	theme	antioxidant	975:985	arg1	activity					987:994	antioxidant activity	975:994	antioxidant activity	975:994	AAP-3-1 exhibited antioxidant activity in a concentration-dependent manner and the scavenging rates at 1.6 mg/mL on superoxide anions and hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals were 88.13%, 93.03% and 68.31%, respectively.
32781121	7	18	theme	reactive	1316:1323	arg1	species					1332:1338	reactive oxygen species	1316:1338	reactive oxygen species generation	1316:1349	AAP-3-1 effectively ameliorated 2,2'-azobis-2-methyl-propanimidamide-induced oxidative stress in HepG2 cells by inhibiting reactive oxygen species generation, decreasing the content of malondialdehyde, and increasing the activities of superoxide dismutase, glutathione peroxidase and catalase.
32781121	1	19	theme	polysaccharide	198:211	arg1	AAP-3-1					213:219	a novel polysaccharide AAP-3-1	190:219	a novel polysaccharide AAP-3-1 from Auricularia auricula	190:245	The objective was to investigate the in vitro antioxidant activity of a novel polysaccharide AAP-3-1 from Auricularia auricula.
32781121	3	20	theme	permeation	555:564	arg1	chromatography					566:579	high performance gel permeation chromatography	534:579	high performance gel permeation chromatography	534:579	The molecular weight and monosaccharide composition were determined by high performance gel permeation chromatography and high-performance liquid chromatography, respectively.
32781121	5	21	theme	320.9 kDa	946:954	arg1	weight					936:941	a molecular weight	924:941	a molecular weight of 320.9 kDa	924:954	The results showed that AAP-3-1 is a heteropolysaccharide and is mainly composed of mannose and glucose in a molar ratio of 1.4:1 with a molecular weight of 320.9 kDa.
32781121	1	22	theme	antioxidant	166:176	arg1	activity					178:185	the in vitro antioxidant activity	153:185	the in vitro antioxidant activity of a novel polysaccharide AAP-3-1 from Auricularia auricula	153:245	The objective was to investigate the in vitro antioxidant activity of a novel polysaccharide AAP-3-1 from Auricularia auricula.
32781121	7	23	theme	HepG2	1290:1294	arg1	cells					1296:1300	HepG2 cells	1290:1300	HepG2 cells	1290:1300	AAP-3-1 effectively ameliorated 2,2'-azobis-2-methyl-propanimidamide-induced oxidative stress in HepG2 cells by inhibiting reactive oxygen species generation, decreasing the content of malondialdehyde, and increasing the activities of superoxide dismutase, glutathione peroxidase and catalase.
32781121	8	24	theme	antioxidant	1518:1528	arg1	mechanism					1530:1538	the antioxidant mechanism	1514:1538	the antioxidant mechanism of AAP-3-1	1514:1549	The results indicated that the antioxidant mechanism of AAP-3-1 was associated with both non-enzymatic and enzymatic defense systems.
32781121	2	25	theme	fruiting	278:285	arg1	bodies					287:292	the fruiting bodies	274:292	the fruiting bodies of A. auricula	274:307	AAP-3-1 was isolated from the fruiting bodies of A. auricula by hot water extraction and ethanol precipitation and was purified by DEAE FF ion exchange chromatography and Superdex 200 gel filtration chromatography.
32781121	2	26	theme	ion	387:389	arg1	chromatography					400:413	DEAE FF ion exchange chromatography	379:413	DEAE FF ion exchange chromatography	379:413	AAP-3-1 was isolated from the fruiting bodies of A. auricula by hot water extraction and ethanol precipitation and was purified by DEAE FF ion exchange chromatography and Superdex 200 gel filtration chromatography.
32781121	7	27	theme	oxygen	1325:1330	arg1	species					1332:1338	reactive oxygen species	1316:1338	reactive oxygen species generation	1316:1349	AAP-3-1 effectively ameliorated 2,2'-azobis-2-methyl-propanimidamide-induced oxidative stress in HepG2 cells by inhibiting reactive oxygen species generation, decreasing the content of malondialdehyde, and increasing the activities of superoxide dismutase, glutathione peroxidase and catalase.
32781121	5	28	theme	molecular	926:934	arg1	weight					936:941	a molecular weight	924:941	a molecular weight of 320.9 kDa	924:954	The results showed that AAP-3-1 is a heteropolysaccharide and is mainly composed of mannose and glucose in a molar ratio of 1.4:1 with a molecular weight of 320.9 kDa.
32781121	7	29	theme	glutathione	1450:1460	arg1	peroxidase					1462:1471	glutathione peroxidase	1450:1471	glutathione peroxidase	1450:1471	AAP-3-1 effectively ameliorated 2,2'-azobis-2-methyl-propanimidamide-induced oxidative stress in HepG2 cells by inhibiting reactive oxygen species generation, decreasing the content of malondialdehyde, and increasing the activities of superoxide dismutase, glutathione peroxidase and catalase.
32781121	2	30	theme	water	316:320	arg1	extraction					322:331	hot water extraction	312:331	hot water extraction	312:331	AAP-3-1 was isolated from the fruiting bodies of A. auricula by hot water extraction and ethanol precipitation and was purified by DEAE FF ion exchange chromatography and Superdex 200 gel filtration chromatography.
32781121	7	31	theme	superoxide	1428:1437	arg1	dismutase					1439:1447	superoxide dismutase	1428:1447	superoxide dismutase	1428:1447	AAP-3-1 effectively ameliorated 2,2'-azobis-2-methyl-propanimidamide-induced oxidative stress in HepG2 cells by inhibiting reactive oxygen species generation, decreasing the content of malondialdehyde, and increasing the activities of superoxide dismutase, glutathione peroxidase and catalase.
32781121	2	32	theme	FF	384:385	arg1	chromatography					400:413	DEAE FF ion exchange chromatography	379:413	DEAE FF ion exchange chromatography	379:413	AAP-3-1 was isolated from the fruiting bodies of A. auricula by hot water extraction and ethanol precipitation and was purified by DEAE FF ion exchange chromatography and Superdex 200 gel filtration chromatography.
32781121	5	33	theme	molar	898:902	arg1	ratio					904:908	a molar ratio	896:908	a molar ratio of 1.4:1 with a molecular weight of 320.9 kDa	896:954	The results showed that AAP-3-1 is a heteropolysaccharide and is mainly composed of mannose and glucose in a molar ratio of 1.4:1 with a molecular weight of 320.9 kDa.
32781121	6	34	theme	superoxide	1073:1082	arg1	anions					1084:1089	superoxide anions	1073:1089	superoxide anions	1073:1089	AAP-3-1 exhibited antioxidant activity in a concentration-dependent manner and the scavenging rates at 1.6 mg/mL on superoxide anions and hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals were 88.13%, 93.03% and 68.31%, respectively.
32781121	2	35	theme	hot	312:314	arg1	extraction					322:331	hot water extraction	312:331	hot water extraction	312:331	AAP-3-1 was isolated from the fruiting bodies of A. auricula by hot water extraction and ethanol precipitation and was purified by DEAE FF ion exchange chromatography and Superdex 200 gel filtration chromatography.
32781121	2	36	theme	DEAE	379:382	arg1	chromatography					400:413	DEAE FF ion exchange chromatography	379:413	DEAE FF ion exchange chromatography	379:413	AAP-3-1 was isolated from the fruiting bodies of A. auricula by hot water extraction and ethanol precipitation and was purified by DEAE FF ion exchange chromatography and Superdex 200 gel filtration chromatography.
32781121	6	37	from	1.6 mg/mL	1060:1068	arg1	%					1157:1157	88.13%	1152:1157	88.13%	1152:1157	AAP-3-1 exhibited antioxidant activity in a concentration-dependent manner and the scavenging rates at 1.6 mg/mL on superoxide anions and hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals were 88.13%, 93.03% and 68.31%, respectively.
32781121	6	37	from	1.6 mg/mL	1060:1068	arg1	rates					1051:1055	the scavenging rates	1036:1055	the scavenging rates at 1.6 mg/mL on superoxide anions and hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals	1036:1145	AAP-3-1 exhibited antioxidant activity in a concentration-dependent manner and the scavenging rates at 1.6 mg/mL on superoxide anions and hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals were 88.13%, 93.03% and 68.31%, respectively.
32781121	8	38	theme	non-enzymatic	1576:1588	arg1	systems					1612:1618	both non-enzymatic and enzymatic defense systems	1571:1618	both non-enzymatic and enzymatic defense systems	1571:1618	The results indicated that the antioxidant mechanism of AAP-3-1 was associated with both non-enzymatic and enzymatic defense systems.
32781121	3	39	theme	liquid	602:607	arg1	chromatography					609:622	high-performance liquid chromatography	585:622	high-performance liquid chromatography	585:622	The molecular weight and monosaccharide composition were determined by high performance gel permeation chromatography and high-performance liquid chromatography, respectively.
32781121	1	40	theme	novel	192:196	arg1	AAP-3-1					213:219	a novel polysaccharide AAP-3-1	190:219	a novel polysaccharide AAP-3-1 from Auricularia auricula	190:245	The objective was to investigate the in vitro antioxidant activity of a novel polysaccharide AAP-3-1 from Auricularia auricula.
32781121	2	41	theme	gel	432:434	arg1	filtration					436:445	Superdex 200 gel filtration	419:445	Superdex 200 gel filtration	419:445	AAP-3-1 was isolated from the fruiting bodies of A. auricula by hot water extraction and ethanol precipitation and was purified by DEAE FF ion exchange chromatography and Superdex 200 gel filtration chromatography.
32781121	2	42	theme	auricula	300:307	arg1	bodies					287:292	the fruiting bodies	274:292	the fruiting bodies of A. auricula	274:307	AAP-3-1 was isolated from the fruiting bodies of A. auricula by hot water extraction and ethanol precipitation and was purified by DEAE FF ion exchange chromatography and Superdex 200 gel filtration chromatography.
32781121	2	43	theme	Superdex	419:426	arg1	filtration					436:445	Superdex 200 gel filtration	419:445	Superdex 200 gel filtration	419:445	AAP-3-1 was isolated from the fruiting bodies of A. auricula by hot water extraction and ethanol precipitation and was purified by DEAE FF ion exchange chromatography and Superdex 200 gel filtration chromatography.
32781121	7	44	theme	peroxidase	1462:1471	arg1	activities					1414:1423	the activities	1410:1423	the activities of superoxide dismutase, glutathione peroxidase and catalase	1410:1484	AAP-3-1 effectively ameliorated 2,2'-azobis-2-methyl-propanimidamide-induced oxidative stress in HepG2 cells by inhibiting reactive oxygen species generation, decreasing the content of malondialdehyde, and increasing the activities of superoxide dismutase, glutathione peroxidase and catalase.
32781121	4	45	theme	electron	726:733	arg1	microscopy					735:744	scanning electron microscopy	717:744	scanning electron microscopy	717:744	Ultraviolet visible spectroscopy, Fourier transform infrared spectroscopy and scanning electron microscopy were used for structural characterization.
32781121	1	46	theme	AAP-3-1	213:219	arg1	activity					178:185	the in vitro antioxidant activity	153:185	the in vitro antioxidant activity of a novel polysaccharide AAP-3-1 from Auricularia auricula	153:245	The objective was to investigate the in vitro antioxidant activity of a novel polysaccharide AAP-3-1 from Auricularia auricula.
32781121	0	47	theme	in	35:36	arg1	activity					56:63	in vitro antioxidant activity	35:63	in vitro antioxidant activity	35:63	Purification, characterization and in vitro antioxidant activity of a polysaccharide AAP-3-1 from Auricularia auricula.
32781121	2	48	dep	chromatography	400:413	arg1	chromatography					447:460	chromatography	447:460	chromatography	447:460	AAP-3-1 was isolated from the fruiting bodies of A. auricula by hot water extraction and ethanol precipitation and was purified by DEAE FF ion exchange chromatography and Superdex 200 gel filtration chromatography.
32781121	4	49	used	used	751:754	arg2	Fourier					673:679	Fourier	673:679	Fourier transform infrared spectroscopy	673:711	Ultraviolet visible spectroscopy, Fourier transform infrared spectroscopy and scanning electron microscopy were used for structural characterization.
32781121	4	49	used	used	751:754	arg2	spectroscopy					659:670	Ultraviolet visible spectroscopy	639:670	Ultraviolet visible spectroscopy	639:670	Ultraviolet visible spectroscopy, Fourier transform infrared spectroscopy and scanning electron microscopy were used for structural characterization.
32781121	4	49	used	used	751:754	arg2	microscopy					735:744	scanning electron microscopy	717:744	scanning electron microscopy	717:744	Ultraviolet visible spectroscopy, Fourier transform infrared spectroscopy and scanning electron microscopy were used for structural characterization.
32781121	3	50	theme	molecular	467:475	arg1	weight					477:482	The molecular weight	463:482	The molecular weight	463:482	The molecular weight and monosaccharide composition were determined by high performance gel permeation chromatography and high-performance liquid chromatography, respectively.
32781121	6	51	theme	scavenging	1040:1049	arg1	%					1157:1157	88.13%	1152:1157	88.13%	1152:1157	AAP-3-1 exhibited antioxidant activity in a concentration-dependent manner and the scavenging rates at 1.6 mg/mL on superoxide anions and hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals were 88.13%, 93.03% and 68.31%, respectively.
32781121	6	51	theme	scavenging	1040:1049	arg1	rates					1051:1055	the scavenging rates	1036:1055	the scavenging rates at 1.6 mg/mL on superoxide anions and hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals	1036:1145	AAP-3-1 exhibited antioxidant activity in a concentration-dependent manner and the scavenging rates at 1.6 mg/mL on superoxide anions and hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals were 88.13%, 93.03% and 68.31%, respectively.
32781121	8	52	theme	AAP-3-1	1543:1549	arg1	mechanism					1530:1538	the antioxidant mechanism	1514:1538	the antioxidant mechanism of AAP-3-1	1514:1549	The results indicated that the antioxidant mechanism of AAP-3-1 was associated with both non-enzymatic and enzymatic defense systems.
32781121	7	53	theme	dismutase	1439:1447	arg1	activities					1414:1423	the activities	1410:1423	the activities of superoxide dismutase, glutathione peroxidase and catalase	1410:1484	AAP-3-1 effectively ameliorated 2,2'-azobis-2-methyl-propanimidamide-induced oxidative stress in HepG2 cells by inhibiting reactive oxygen species generation, decreasing the content of malondialdehyde, and increasing the activities of superoxide dismutase, glutathione peroxidase and catalase.
32781121	0	54	theme	antioxidant	44:54	arg1	activity					56:63	in vitro antioxidant activity	35:63	in vitro antioxidant activity	35:63	Purification, characterization and in vitro antioxidant activity of a polysaccharide AAP-3-1 from Auricularia auricula.
32781121	2	55	theme	exchange	391:398	arg1	chromatography					400:413	DEAE FF ion exchange chromatography	379:413	DEAE FF ion exchange chromatography	379:413	AAP-3-1 was isolated from the fruiting bodies of A. auricula by hot water extraction and ethanol precipitation and was purified by DEAE FF ion exchange chromatography and Superdex 200 gel filtration chromatography.
32781121	0	56	dep	in	35:36	arg1	vitro					38:42	vitro	38:42	vitro	38:42	Purification, characterization and in vitro antioxidant activity of a polysaccharide AAP-3-1 from Auricularia auricula.
32781121	2	57	theme	ethanol	337:343	arg1	precipitation					345:357	ethanol precipitation	337:357	ethanol precipitation	337:357	AAP-3-1 was isolated from the fruiting bodies of A. auricula by hot water extraction and ethanol precipitation and was purified by DEAE FF ion exchange chromatography and Superdex 200 gel filtration chromatography.
32781121	7	58	from	stress	1280:1285	arg1	cells					1296:1300	HepG2 cells	1290:1300	HepG2 cells	1290:1300	AAP-3-1 effectively ameliorated 2,2'-azobis-2-methyl-propanimidamide-induced oxidative stress in HepG2 cells by inhibiting reactive oxygen species generation, decreasing the content of malondialdehyde, and increasing the activities of superoxide dismutase, glutathione peroxidase and catalase.
32781121	6	59	from	rates	1051:1055	arg1	radicals					1138:1145	hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals	1095:1145	radicals	1138:1145	AAP-3-1 exhibited antioxidant activity in a concentration-dependent manner and the scavenging rates at 1.6 mg/mL on superoxide anions and hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals were 88.13%, 93.03% and 68.31%, respectively.
32781121	6	59	from	rates	1051:1055	arg1	anions					1084:1089	superoxide anions	1073:1089	superoxide anions	1073:1089	AAP-3-1 exhibited antioxidant activity in a concentration-dependent manner and the scavenging rates at 1.6 mg/mL on superoxide anions and hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals were 88.13%, 93.03% and 68.31%, respectively.
32781121	0	60	from	auricula	110:117	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Purification, characterization and in vitro antioxidant activity of a polysaccharide AAP-3-1 from Auricularia auricula.
32781121	0	60	from	auricula	110:117	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, characterization and in vitro antioxidant activity of a polysaccharide AAP-3-1 from Auricularia auricula.
32781121	0	60	from	auricula	110:117	arg1	activity					56:63	in vitro antioxidant activity	35:63	in vitro antioxidant activity	35:63	Purification, characterization and in vitro antioxidant activity of a polysaccharide AAP-3-1 from Auricularia auricula.
32781121	8	61	theme	defense	1604:1610	arg1	systems					1612:1618	both non-enzymatic and enzymatic defense systems	1571:1618	both non-enzymatic and enzymatic defense systems	1571:1618	The results indicated that the antioxidant mechanism of AAP-3-1 was associated with both non-enzymatic and enzymatic defense systems.
32781121	3	62	theme	monosaccharide	488:501	arg1	composition					503:513	monosaccharide composition	488:513	monosaccharide composition	488:513	The molecular weight and monosaccharide composition were determined by high performance gel permeation chromatography and high-performance liquid chromatography, respectively.
32781121	1	63	from	auricula	238:245	arg1	AAP-3-1					213:219	a novel polysaccharide AAP-3-1	190:219	a novel polysaccharide AAP-3-1 from Auricularia auricula	190:245	The objective was to investigate the in vitro antioxidant activity of a novel polysaccharide AAP-3-1 from Auricularia auricula.
32781121	1	63	from	auricula	238:245	arg1	activity					178:185	the in vitro antioxidant activity	153:185	the in vitro antioxidant activity of a novel polysaccharide AAP-3-1 from Auricularia auricula	153:245	The objective was to investigate the in vitro antioxidant activity of a novel polysaccharide AAP-3-1 from Auricularia auricula.
32781121	4	64	dep	Fourier	673:679	arg1	transform					681:689	transform	681:689	transform infrared spectroscopy	681:711	Ultraviolet visible spectroscopy, Fourier transform infrared spectroscopy and scanning electron microscopy were used for structural characterization.
32781121	8	65	theme	enzymatic	1594:1602	arg1	systems					1612:1618	both non-enzymatic and enzymatic defense systems	1571:1618	both non-enzymatic and enzymatic defense systems	1571:1618	The results indicated that the antioxidant mechanism of AAP-3-1 was associated with both non-enzymatic and enzymatic defense systems.
32781121	4	66	theme	structural	760:769	arg1	characterization					771:786	structural characterization	760:786	structural characterization	760:786	Ultraviolet visible spectroscopy, Fourier transform infrared spectroscopy and scanning electron microscopy were used for structural characterization.
32781121	7	67	theme	malondialdehyde	1378:1392	arg1	content					1367:1373	the content	1363:1373	the content of malondialdehyde	1363:1392	AAP-3-1 effectively ameliorated 2,2'-azobis-2-methyl-propanimidamide-induced oxidative stress in HepG2 cells by inhibiting reactive oxygen species generation, decreasing the content of malondialdehyde, and increasing the activities of superoxide dismutase, glutathione peroxidase and catalase.
32781121	4	68	theme	scanning	717:724	arg1	microscopy					735:744	scanning electron microscopy	717:744	scanning electron microscopy	717:744	Ultraviolet visible spectroscopy, Fourier transform infrared spectroscopy and scanning electron microscopy were used for structural characterization.
32781121	5	69	theme	1.4:1	913:917	arg1	ratio					904:908	a molar ratio	896:908	a molar ratio of 1.4:1 with a molecular weight of 320.9 kDa	896:954	The results showed that AAP-3-1 is a heteropolysaccharide and is mainly composed of mannose and glucose in a molar ratio of 1.4:1 with a molecular weight of 320.9 kDa.
32781121	3	70	theme	performance	539:549	arg1	chromatography					566:579	high performance gel permeation chromatography	534:579	high performance gel permeation chromatography	534:579	The molecular weight and monosaccharide composition were determined by high performance gel permeation chromatography and high-performance liquid chromatography, respectively.
32781121	5	71	from	glucose	885:891	arg1	ratio					904:908	a molar ratio	896:908	a molar ratio of 1.4:1 with a molecular weight of 320.9 kDa	896:954	The results showed that AAP-3-1 is a heteropolysaccharide and is mainly composed of mannose and glucose in a molar ratio of 1.4:1 with a molecular weight of 320.9 kDa.
32781121	6	72	theme	1,1-diphenyl-2-picrylhydrazyl	1108:1136	arg1	radicals					1138:1145	hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals	1095:1145	radicals	1138:1145	AAP-3-1 exhibited antioxidant activity in a concentration-dependent manner and the scavenging rates at 1.6 mg/mL on superoxide anions and hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals were 88.13%, 93.03% and 68.31%, respectively.
32781121	3	73	theme	gel	551:553	arg1	chromatography					566:579	high performance gel permeation chromatography	534:579	high performance gel permeation chromatography	534:579	The molecular weight and monosaccharide composition were determined by high performance gel permeation chromatography and high-performance liquid chromatography, respectively.
32781121	7	74	theme	species	1332:1338	arg1	generation					1340:1349	reactive oxygen species generation	1316:1349	reactive oxygen species generation	1316:1349	AAP-3-1 effectively ameliorated 2,2'-azobis-2-methyl-propanimidamide-induced oxidative stress in HepG2 cells by inhibiting reactive oxygen species generation, decreasing the content of malondialdehyde, and increasing the activities of superoxide dismutase, glutathione peroxidase and catalase.
32781121	2	75	attach	isolated	260:267	arg2	AAP-3-1					248:254	AAP-3-1	248:254	AAP-3-1	248:254	AAP-3-1 was isolated from the fruiting bodies of A. auricula by hot water extraction and ethanol precipitation and was purified by DEAE FF ion exchange chromatography and Superdex 200 gel filtration chromatography.
32781121	2	75	attach	isolated	260:267	arg1	bodies					287:292	the fruiting bodies	274:292	the fruiting bodies of A. auricula	274:307	AAP-3-1 was isolated from the fruiting bodies of A. auricula by hot water extraction and ethanol precipitation and was purified by DEAE FF ion exchange chromatography and Superdex 200 gel filtration chromatography.
33357891	3	0	theme	MAPP	670:673	arg1	agent					684:688	MAPP coupling agent	670:688	MAPP coupling agent	670:688	Moreover, combining XG/CNC modified interphases with MAPP coupling agent enhances the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture, and modifies failure mechanisms as revealed by in situ micro-mechanical tensile SEM experiments.
33357891	3	1	theme	micro-mechanical	856:871	arg1	experiments					885:895	in situ micro-mechanical tensile SEM experiments	848:895	in situ micro-mechanical tensile SEM experiments	848:895	Moreover, combining XG/CNC modified interphases with MAPP coupling agent enhances the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture, and modifies failure mechanisms as revealed by in situ micro-mechanical tensile SEM experiments.
33357891	2	2	theme	wettability	449:459	arg1	study					486:490	The wettability and interfacial adhesion study	445:490	The wettability and interfacial adhesion study	445:490	The wettability and interfacial adhesion study reveals a strong decrease in the polar character of CNC modified flax fibres, hence increasing the work of adhesion with PP.
33357891	1	3	theme	biomass	333:339	arg1	by-products					341:351	biomass by-products	333:351	biomass by-products	333:351	This work is focused on the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres.
33357891	3	4	with	biocomposites	737:749	arg1	work					784:787	work	784:787	work	784:787	Moreover, combining XG/CNC modified interphases with MAPP coupling agent enhances the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture, and modifies failure mechanisms as revealed by in situ micro-mechanical tensile SEM experiments.
33357891	3	4	with	biocomposites	737:749	arg1	strength					771:778	higher tensile strength	756:778	higher tensile strength	756:778	Moreover, combining XG/CNC modified interphases with MAPP coupling agent enhances the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture, and modifies failure mechanisms as revealed by in situ micro-mechanical tensile SEM experiments.
33357891	1	5	theme	interphase	168:177	arg1	zone					179:182	the interphase zone	164:182	the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres	164:442	This work is focused on the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres.
33357891	1	6	theme	bio-inspired	242:253	arg1	modification					255:266	a bio-inspired modification	240:266	a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres	240:442	This work is focused on the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres.
33357891	2	7	theme	strong	502:507	arg1	decrease					509:516	a strong decrease	500:516	a strong decrease in the polar character of CNC modified flax fibres	500:567	The wettability and interfacial adhesion study reveals a strong decrease in the polar character of CNC modified flax fibres, hence increasing the work of adhesion with PP.
33357891	1	8	theme	by-products	341:351	arg1	adsorption					319:328	the adsorption	315:328	the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG),	315:407	This work is focused on the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres.
33357891	2	9	theme	fibres	562:567	arg1	character					531:539	the polar character	521:539	the polar character of CNC modified flax fibres	521:567	The wettability and interfacial adhesion study reveals a strong decrease in the polar character of CNC modified flax fibres, hence increasing the work of adhesion with PP.
33357891	4	10	with	composites	1030:1039	arg1	properties					1066:1075	enhanced structural properties	1046:1075	enhanced structural properties	1046:1075	Bio-based hierarchical composites inspired by naturally occurring nanostructures open a new path for the development of sustainable composites with enhanced structural properties.
33357891	3	11	theme	in	848:849	arg1	experiments					885:895	in situ micro-mechanical tensile SEM experiments	848:895	in situ micro-mechanical tensile SEM experiments	848:895	Moreover, combining XG/CNC modified interphases with MAPP coupling agent enhances the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture, and modifies failure mechanisms as revealed by in situ micro-mechanical tensile SEM experiments.
33357891	3	12	theme	rupture	792:798	arg1	work					784:787	work	784:787	work	784:787	Moreover, combining XG/CNC modified interphases with MAPP coupling agent enhances the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture, and modifies failure mechanisms as revealed by in situ micro-mechanical tensile SEM experiments.
33357891	3	12	theme	rupture	792:798	arg1	strength					771:778	higher tensile strength	756:778	higher tensile strength	756:778	Moreover, combining XG/CNC modified interphases with MAPP coupling agent enhances the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture, and modifies failure mechanisms as revealed by in situ micro-mechanical tensile SEM experiments.
33357891	3	13	theme	modified	644:651	arg1	interphases					653:663	XG/CNC modified interphases	637:663	XG/CNC modified interphases	637:663	Moreover, combining XG/CNC modified interphases with MAPP coupling agent enhances the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture, and modifies failure mechanisms as revealed by in situ micro-mechanical tensile SEM experiments.
33357891	2	14	theme	flax	557:560	arg1	fibres					562:567	CNC modified flax fibres	544:567	CNC modified flax fibres	544:567	The wettability and interfacial adhesion study reveals a strong decrease in the polar character of CNC modified flax fibres, hence increasing the work of adhesion with PP.
33357891	1	15	from	modification	148:159	arg1	fibres					198:203	short flax fibres	187:203	short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres	187:442	This work is focused on the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres.
33357891	1	16	theme	hierarchical	419:430	arg1	fibres					437:442	hierarchical flax fibres	419:442	hierarchical flax fibres	419:442	This work is focused on the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres.
33357891	1	17	theme	zone	179:182	arg1	modification					148:159	the modification	144:159	the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres	144:442	This work is focused on the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres.
33357891	4	18	theme	occurring	954:962	arg1	nanostructures					964:977	naturally occurring nanostructures	944:977	naturally occurring nanostructures	944:977	Bio-based hierarchical composites inspired by naturally occurring nanostructures open a new path for the development of sustainable composites with enhanced structural properties.
33357891	1	19	theme	cellulose	359:367	arg1	CNC					383:385	CNC	383:385	CNC	383:385	This work is focused on the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres.
33357891	1	19	theme	cellulose	359:367	arg1	nanocrystals					369:380	cellulose nanocrystals	359:380	cellulose nanocrystals (CNC)	359:386	This work is focused on the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres.
33357891	1	20	theme	flax	432:435	arg1	fibres					437:442	hierarchical flax fibres	419:442	hierarchical flax fibres	419:442	This work is focused on the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres.
33357891	4	21	theme	sustainable	1018:1028	arg1	composites					1030:1039	sustainable composites	1018:1039	sustainable composites with enhanced structural properties	1018:1075	Bio-based hierarchical composites inspired by naturally occurring nanostructures open a new path for the development of sustainable composites with enhanced structural properties.
33357891	3	22	theme	tensile	873:879	arg1	experiments					885:895	in situ micro-mechanical tensile SEM experiments	848:895	in situ micro-mechanical tensile SEM experiments	848:895	Moreover, combining XG/CNC modified interphases with MAPP coupling agent enhances the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture, and modifies failure mechanisms as revealed by in situ micro-mechanical tensile SEM experiments.
33357891	4	23	theme	composites	1030:1039	arg1	development					1003:1013	the development	999:1013	the development of sustainable composites with enhanced structural properties	999:1075	Bio-based hierarchical composites inspired by naturally occurring nanostructures open a new path for the development of sustainable composites with enhanced structural properties.
33357891	3	24	theme	mechanical	712:721	arg1	properties					723:732	the ultimate mechanical properties	699:732	the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture	699:798	Moreover, combining XG/CNC modified interphases with MAPP coupling agent enhances the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture, and modifies failure mechanisms as revealed by in situ micro-mechanical tensile SEM experiments.
33357891	3	25	dep	in	848:849	arg1	situ					851:854	situ	851:854	situ	851:854	Moreover, combining XG/CNC modified interphases with MAPP coupling agent enhances the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture, and modifies failure mechanisms as revealed by in situ micro-mechanical tensile SEM experiments.
33357891	3	26	theme	SEM	881:883	arg1	experiments					885:895	in situ micro-mechanical tensile SEM experiments	848:895	in situ micro-mechanical tensile SEM experiments	848:895	Moreover, combining XG/CNC modified interphases with MAPP coupling agent enhances the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture, and modifies failure mechanisms as revealed by in situ micro-mechanical tensile SEM experiments.
33357891	1	27	theme	short	187:191	arg1	fibres					198:203	short flax fibres	187:203	short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres	187:442	This work is focused on the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres.
33357891	0	28	theme	Hierarchical	0:11	arg1	thermoplastic					13:25	Hierarchical thermoplastic	0:25	Hierarchical thermoplastic	0:25	Hierarchical thermoplastic biocomposites reinforced with flax fibres modified by xyloglucan and cellulose nanocrystals.
33357891	1	29	theme	fibres	271:276	arg1	modification					255:266	a bio-inspired modification	240:266	a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres	240:442	This work is focused on the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres.
33357891	1	30	theme	flax	193:196	arg1	fibres					198:203	short flax fibres	187:203	short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres	187:442	This work is focused on the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres.
33357891	4	31	theme	enhanced	1046:1053	arg1	properties					1066:1075	enhanced structural properties	1046:1075	enhanced structural properties	1046:1075	Bio-based hierarchical composites inspired by naturally occurring nanostructures open a new path for the development of sustainable composites with enhanced structural properties.
33357891	2	32	theme	polar	525:529	arg1	character					531:539	the polar character	521:539	the polar character of CNC modified flax fibres	521:567	The wettability and interfacial adhesion study reveals a strong decrease in the polar character of CNC modified flax fibres, hence increasing the work of adhesion with PP.
33357891	2	33	theme	adhesion	599:606	arg1	work					591:594	the work	587:594	the work of adhesion with PP	587:614	The wettability and interfacial adhesion study reveals a strong decrease in the polar character of CNC modified flax fibres, hence increasing the work of adhesion with PP.
33357891	0	34	dep	xyloglucan	81:90	arg1	nanocrystals					106:117	nanocrystals	106:117	nanocrystals	106:117	Hierarchical thermoplastic biocomposites reinforced with flax fibres modified by xyloglucan and cellulose nanocrystals.
33357891	3	35	theme	ultimate	703:710	arg1	properties					723:732	the ultimate mechanical properties	699:732	the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture	699:798	Moreover, combining XG/CNC modified interphases with MAPP coupling agent enhances the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture, and modifies failure mechanisms as revealed by in situ micro-mechanical tensile SEM experiments.
33357891	4	36	theme	Bio-based	898:906	arg1	composites					921:930	Bio-based hierarchical composites	898:930	Bio-based hierarchical composites inspired by naturally occurring nanostructures	898:977	Bio-based hierarchical composites inspired by naturally occurring nanostructures open a new path for the development of sustainable composites with enhanced structural properties.
33357891	0	37	theme	flax	57:60	arg1	fibres					62:67	flax fibres	57:67	flax fibres modified by xyloglucan and cellulose nanocrystals	57:117	Hierarchical thermoplastic biocomposites reinforced with flax fibres modified by xyloglucan and cellulose nanocrystals.
33357891	1	38	theme	nanostructuration	286:302	arg1	"					303:303	nanostructuration"	286:303	nanostructuration"	286:303	This work is focused on the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres.
33357891	2	39	with	work	591:594	arg1	PP					613:614	PP	613:614	PP	613:614	The wettability and interfacial adhesion study reveals a strong decrease in the polar character of CNC modified flax fibres, hence increasing the work of adhesion with PP.
33357891	1	40	theme	polypropylene	207:219	arg1	composites					226:235	polypropylene (PP) composites	207:235	short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres	187:442	This work is focused on the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres.
33357891	3	41	theme	higher	756:761	arg1	strength					771:778	higher tensile strength	756:778	higher tensile strength	756:778	Moreover, combining XG/CNC modified interphases with MAPP coupling agent enhances the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture, and modifies failure mechanisms as revealed by in situ micro-mechanical tensile SEM experiments.
33357891	1	42	dep	nanocrystals	369:380	arg1	i.e.					354:357	i.e.	354:357	i.e.	354:357	This work is focused on the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres.
33357891	2	43	theme	modified	548:555	arg1	fibres					562:567	CNC modified flax fibres	544:567	CNC modified flax fibres	544:567	The wettability and interfacial adhesion study reveals a strong decrease in the polar character of CNC modified flax fibres, hence increasing the work of adhesion with PP.
33357891	3	44	theme	coupling	675:682	arg1	agent					684:688	MAPP coupling agent	670:688	MAPP coupling agent	670:688	Moreover, combining XG/CNC modified interphases with MAPP coupling agent enhances the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture, and modifies failure mechanisms as revealed by in situ micro-mechanical tensile SEM experiments.
33357891	3	45	theme	biocomposites	737:749	arg1	properties					723:732	the ultimate mechanical properties	699:732	the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture	699:798	Moreover, combining XG/CNC modified interphases with MAPP coupling agent enhances the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture, and modifies failure mechanisms as revealed by in situ micro-mechanical tensile SEM experiments.
33357891	2	46	theme	CNC	544:546	arg1	fibres					562:567	CNC modified flax fibres	544:567	CNC modified flax fibres	544:567	The wettability and interfacial adhesion study reveals a strong decrease in the polar character of CNC modified flax fibres, hence increasing the work of adhesion with PP.
33357891	1	47	from	fibres	198:203	arg1	modification					148:159	the modification	144:159	the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres	144:442	This work is focused on the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres.
33357891	3	48	theme	XG/CNC	637:642	arg1	interphases					653:663	XG/CNC modified interphases	637:663	XG/CNC modified interphases	637:663	Moreover, combining XG/CNC modified interphases with MAPP coupling agent enhances the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture, and modifies failure mechanisms as revealed by in situ micro-mechanical tensile SEM experiments.
33357891	4	49	theme	new	986:988	arg1	path					990:993	a new path	984:993	a new path for the development of sustainable composites with enhanced structural properties	984:1075	Bio-based hierarchical composites inspired by naturally occurring nanostructures open a new path for the development of sustainable composites with enhanced structural properties.
33357891	4	50	theme	structural	1055:1064	arg1	properties					1066:1075	enhanced structural properties	1046:1075	enhanced structural properties	1046:1075	Bio-based hierarchical composites inspired by naturally occurring nanostructures open a new path for the development of sustainable composites with enhanced structural properties.
33357891	0	51	dep	biocomposites	27:39	arg1	reinforced					41:50	reinforced	41:50	biocomposites reinforced with flax fibres modified by xyloglucan and cellulose nanocrystals	27:117	Hierarchical thermoplastic biocomposites reinforced with flax fibres modified by xyloglucan and cellulose nanocrystals.
33357891	3	52	theme	failure	814:820	arg1	mechanisms					822:831	failure mechanisms	814:831	failure mechanisms	814:831	Moreover, combining XG/CNC modified interphases with MAPP coupling agent enhances the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture, and modifies failure mechanisms as revealed by in situ micro-mechanical tensile SEM experiments.
33357891	2	53	from	decrease	509:516	arg1	character					531:539	the polar character	521:539	the polar character of CNC modified flax fibres	521:567	The wettability and interfacial adhesion study reveals a strong decrease in the polar character of CNC modified flax fibres, hence increasing the work of adhesion with PP.
33357891	2	54	theme	adhesion	477:484	arg1	study					486:490	The wettability and interfacial adhesion study	445:490	The wettability and interfacial adhesion study	445:490	The wettability and interfacial adhesion study reveals a strong decrease in the polar character of CNC modified flax fibres, hence increasing the work of adhesion with PP.
33357891	4	55	theme	hierarchical	908:919	arg1	composites					921:930	Bio-based hierarchical composites	898:930	Bio-based hierarchical composites inspired by naturally occurring nanostructures	898:977	Bio-based hierarchical composites inspired by naturally occurring nanostructures open a new path for the development of sustainable composites with enhanced structural properties.
33357891	1	56	dep	fibres	198:203	arg1	composites					226:235	polypropylene (PP) composites	207:235	short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres	187:442	This work is focused on the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres.
33357891	2	57	theme	interfacial	465:475	arg1	study					486:490	The wettability and interfacial adhesion study	445:490	The wettability and interfacial adhesion study	445:490	The wettability and interfacial adhesion study reveals a strong decrease in the polar character of CNC modified flax fibres, hence increasing the work of adhesion with PP.
33357891	1	58	from	zone	179:182	arg1	fibres					198:203	short flax fibres	187:203	short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres	187:442	This work is focused on the modification of the interphase zone in short flax fibres / polypropylene (PP) composites by a bio-inspired modification of fibres called "nanostructuration" that uses the adsorption of biomass by-products, i.e. cellulose nanocrystals (CNC) and xyloglucan (XG), to create hierarchical flax fibres.
33357891	3	59	theme	tensile	763:769	arg1	strength					771:778	higher tensile strength	756:778	higher tensile strength	756:778	Moreover, combining XG/CNC modified interphases with MAPP coupling agent enhances the ultimate mechanical properties of biocomposites with higher tensile strength and work of rupture, and modifies failure mechanisms as revealed by in situ micro-mechanical tensile SEM experiments.
34838853	0	0	theme	moisture	51:58	arg1	properties					71:80	the mechanical, thermal, and moisture absorption properties	22:80	properties	71:80	Effect of palm wax on the mechanical, thermal, and moisture absorption properties of thermoplastic cassava starch composites.
34838853	3	1	theme	current	317:323	arg1	work					325:328	this current work	312:328	this current work	312:328	In this current work, the characteristics of thermoplastic cassava starch (TPCS) containing palm wax at various loading were evaluated.
34838853	5	2	theme	Fourier-transform	678:694	arg1	FT-IR					719:723	FT-IR	719:723	FT-IR	719:723	Next, characterization via scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), mechanical, water solubility, thickness swelling and moisture absorption tests, were conducted on the samples.
34838853	5	2	theme	Fourier-transform	678:694	arg1	spectroscopy					705:716	Fourier-transform infrared spectroscopy	678:716	Fourier-transform infrared spectroscopy (FT-IR)	678:724	Next, characterization via scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), mechanical, water solubility, thickness swelling and moisture absorption tests, were conducted on the samples.
34838853	9	3	theme	thermal	1272:1278	arg1	stability					1280:1288	The thermal stability	1268:1288	The thermal stability of TPCS	1268:1296	The thermal stability of TPCS has improved with rising palm wax content.
34838853	10	4	theme	moisture	1401:1408	arg1	value					1421:1425	the lowest moisture absorption value	1390:1425	the lowest moisture absorption value among the samples	1390:1443	The incorporation of 15 wt% palm wax resulted in the lowest moisture absorption value among the samples.
34838853	5	5	theme	infrared	696:703	arg1	FT-IR					719:723	FT-IR	719:723	FT-IR	719:723	Next, characterization via scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), mechanical, water solubility, thickness swelling and moisture absorption tests, were conducted on the samples.
34838853	5	5	theme	infrared	696:703	arg1	spectroscopy					705:716	Fourier-transform infrared spectroscopy	678:716	Fourier-transform infrared spectroscopy (FT-IR)	678:724	Next, characterization via scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), mechanical, water solubility, thickness swelling and moisture absorption tests, were conducted on the samples.
34838853	10	6	theme	lowest	1394:1399	arg1	value					1421:1425	the lowest moisture absorption value	1390:1425	the lowest moisture absorption value among the samples	1390:1443	The incorporation of 15 wt% palm wax resulted in the lowest moisture absorption value among the samples.
34838853	10	7	theme	15 wt	1362:1366	arg1	wax					1374:1376	15 wt% palm wax	1362:1376	15 wt% palm wax	1362:1376	The incorporation of 15 wt% palm wax resulted in the lowest moisture absorption value among the samples.
34838853	7	8	theme	cleavage	1073:1080	arg1	fracture					1082:1089	irregular and rougher cleavage fracture	1051:1089	irregular and rougher cleavage fracture	1051:1089	Besides, the morphology of the samples demonstrated irregular and rougher cleavage fracture after palm wax addition.
34838853	3	9	theme	thermoplastic	354:366	arg1	TPCS					384:387	TPCS	384:387	TPCS	384:387	In this current work, the characteristics of thermoplastic cassava starch (TPCS) containing palm wax at various loading were evaluated.
34838853	3	9	theme	thermoplastic	354:366	arg1	starch					376:381	thermoplastic cassava starch	354:381	thermoplastic cassava starch (TPCS) containing palm wax at various loading	354:427	In this current work, the characteristics of thermoplastic cassava starch (TPCS) containing palm wax at various loading were evaluated.
34838853	0	10	from	Effect	0:5	arg1	properties					71:80	the mechanical, thermal, and moisture absorption properties	22:80	properties	71:80	Effect of palm wax on the mechanical, thermal, and moisture absorption properties of thermoplastic cassava starch composites.
34838853	0	10	from	Effect	0:5	arg1	thermal					38:44	thermal	38:44	thermal	38:44	Effect of palm wax on the mechanical, thermal, and moisture absorption properties of thermoplastic cassava starch composites.
34838853	3	11	contain	containing	390:399	arg1	TPCS					384:387	TPCS	384:387	TPCS	384:387	In this current work, the characteristics of thermoplastic cassava starch (TPCS) containing palm wax at various loading were evaluated.
34838853	3	11	contain	containing	390:399	arg1	starch					376:381	thermoplastic cassava starch	354:381	thermoplastic cassava starch (TPCS) containing palm wax at various loading	354:427	In this current work, the characteristics of thermoplastic cassava starch (TPCS) containing palm wax at various loading were evaluated.
34838853	3	11	contain	containing	390:399	arg2	wax					406:408	palm wax	401:408	palm wax	401:408	In this current work, the characteristics of thermoplastic cassava starch (TPCS) containing palm wax at various loading were evaluated.
34838853	2	12	theme	moisture	287:294	arg1	sensitivity					296:306	high moisture sensitivity	282:306	high moisture sensitivity	282:306	However, it possesses some limitations in terms of mechanical performance and high moisture sensitivity.
34838853	11	13	theme	developed	1459:1467	arg1	wax					1479:1481	the developed TPCS/palm wax	1455:1481	the developed TPCS/palm wax with improved mechanical and moisture resistance characteristics	1455:1546	Overall, the developed TPCS/palm wax with improved mechanical and moisture resistance characteristics has the potential to be used as biodegradable materials.
34838853	3	14	theme	cassava	368:374	arg1	TPCS					384:387	TPCS	384:387	TPCS	384:387	In this current work, the characteristics of thermoplastic cassava starch (TPCS) containing palm wax at various loading were evaluated.
34838853	3	14	theme	cassava	368:374	arg1	starch					376:381	thermoplastic cassava starch	354:381	thermoplastic cassava starch (TPCS) containing palm wax at various loading	354:427	In this current work, the characteristics of thermoplastic cassava starch (TPCS) containing palm wax at various loading were evaluated.
34838853	4	15	theme	hot	467:469	arg1	pressing					471:478	hot pressing	467:478	hot pressing	467:478	TPCS was prepared via hot pressing by varying the ratios of palm wax (2.5, 5, 10, and 15 wt%).
34838853	7	16	theme	rougher	1065:1071	arg1	fracture					1082:1089	irregular and rougher cleavage fracture	1051:1089	irregular and rougher cleavage fracture	1051:1089	Besides, the morphology of the samples demonstrated irregular and rougher cleavage fracture after palm wax addition.
34838853	2	17	theme	high	282:285	arg1	sensitivity					296:306	high moisture sensitivity	282:306	high moisture sensitivity	282:306	However, it possesses some limitations in terms of mechanical performance and high moisture sensitivity.
34838853	9	18	theme	wax	1328:1330	arg1	content					1332:1338	palm wax content	1323:1338	palm wax content	1323:1338	The thermal stability of TPCS has improved with rising palm wax content.
34838853	6	19	theme	mechanical	943:952	arg1	characteristics					954:968	mechanical characteristics	943:968	mechanical characteristics of the thermoplastic blends	943:996	The findings showed that incorporating starch-based thermoplastics with palm wax has remarkably improved mechanical characteristics of the thermoplastic blends.
34838853	0	20	theme	thermoplastic	85:97	arg1	composites					114:123	thermoplastic cassava starch composites	85:123	thermoplastic cassava starch composites	85:123	Effect of palm wax on the mechanical, thermal, and moisture absorption properties of thermoplastic cassava starch composites.
34838853	8	21	theme	hydrogen	1226:1233	arg1	bonds					1235:1239	the intermolecular hydrogen bonds	1207:1239	the intermolecular hydrogen bonds that existed between them	1207:1265	FT-IR indicated the existence of intermolecular interaction between TPCS and palm wax with the intermolecular hydrogen bonds that existed between them.
34838853	5	22	theme	absorption	789:798	arg1	tests					800:804	moisture absorption tests	780:804	moisture absorption tests	780:804	Next, characterization via scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), mechanical, water solubility, thickness swelling and moisture absorption tests, were conducted on the samples.
34838853	7	23	theme	palm	1097:1100	arg1	addition					1106:1113	palm wax addition	1097:1113	palm wax addition	1097:1113	Besides, the morphology of the samples demonstrated irregular and rougher cleavage fracture after palm wax addition.
34838853	7	24	theme	irregular	1051:1059	arg1	fracture					1082:1089	irregular and rougher cleavage fracture	1051:1089	irregular and rougher cleavage fracture	1051:1089	Besides, the morphology of the samples demonstrated irregular and rougher cleavage fracture after palm wax addition.
34838853	2	25	theme	performance	266:276	arg1	terms					246:250	terms	246:250	terms of mechanical performance and high moisture sensitivity	246:306	However, it possesses some limitations in terms of mechanical performance and high moisture sensitivity.
34838853	2	26	from	limitations	231:241	arg1	terms					246:250	terms	246:250	terms of mechanical performance and high moisture sensitivity	246:306	However, it possesses some limitations in terms of mechanical performance and high moisture sensitivity.
34838853	1	27	theme	sustainable	164:174	arg1	starch					140:145	Thermoplastic starch	126:145	Thermoplastic starch	126:145	Thermoplastic starch is a potentially sustainable and biodegradable material.
34838853	1	27	theme	sustainable	164:174	arg1	material					194:201	a potentially sustainable and biodegradable material	150:201	a potentially sustainable and biodegradable material	150:201	Thermoplastic starch is a potentially sustainable and biodegradable material.
34838853	9	28	theme	TPCS	1293:1296	arg1	stability					1280:1288	The thermal stability	1268:1288	The thermal stability of TPCS	1268:1296	The thermal stability of TPCS has improved with rising palm wax content.
34838853	5	29	theme	scanning	567:574	arg1	SEM					597:599	SEM	597:599	SEM	597:599	Next, characterization via scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), mechanical, water solubility, thickness swelling and moisture absorption tests, were conducted on the samples.
34838853	5	29	theme	scanning	567:574	arg1	microscopy					585:594	scanning electron microscopy	567:594	scanning electron microscopy (SEM)	567:600	Next, characterization via scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), mechanical, water solubility, thickness swelling and moisture absorption tests, were conducted on the samples.
34838853	10	30	theme	wax	1374:1376	arg1	incorporation					1345:1357	The incorporation	1341:1357	The incorporation of 15 wt% palm wax	1341:1376	The incorporation of 15 wt% palm wax resulted in the lowest moisture absorption value among the samples.
34838853	3	31	theme	various	413:419	arg1	loading					421:427	various loading	413:427	various loading	413:427	In this current work, the characteristics of thermoplastic cassava starch (TPCS) containing palm wax at various loading were evaluated.
34838853	6	32	theme	palm	910:913	arg1	wax					915:917	palm wax	910:917	palm wax	910:917	The findings showed that incorporating starch-based thermoplastics with palm wax has remarkably improved mechanical characteristics of the thermoplastic blends.
34838853	5	33	theme	electron	576:583	arg1	SEM					597:599	SEM	597:599	SEM	597:599	Next, characterization via scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), mechanical, water solubility, thickness swelling and moisture absorption tests, were conducted on the samples.
34838853	5	33	theme	electron	576:583	arg1	microscopy					585:594	scanning electron microscopy	567:594	scanning electron microscopy (SEM)	567:600	Next, characterization via scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), mechanical, water solubility, thickness swelling and moisture absorption tests, were conducted on the samples.
34838853	10	34	theme	palm	1369:1372	arg1	wax					1374:1376	15 wt% palm wax	1362:1376	15 wt% palm wax	1362:1376	The incorporation of 15 wt% palm wax resulted in the lowest moisture absorption value among the samples.
34838853	5	35	theme	scanning	650:657	arg1	DSC					672:674	DSC	672:674	DSC	672:674	Next, characterization via scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), mechanical, water solubility, thickness swelling and moisture absorption tests, were conducted on the samples.
34838853	5	35	theme	scanning	650:657	arg1	calorimetry					659:669	differential scanning calorimetry	637:669	differential scanning calorimetry (DSC)	637:675	Next, characterization via scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), mechanical, water solubility, thickness swelling and moisture absorption tests, were conducted on the samples.
34838853	1	36	theme	biodegradable	180:192	arg1	starch					140:145	Thermoplastic starch	126:145	Thermoplastic starch	126:145	Thermoplastic starch is a potentially sustainable and biodegradable material.
34838853	1	36	theme	biodegradable	180:192	arg1	material					194:201	a potentially sustainable and biodegradable material	150:201	a potentially sustainable and biodegradable material	150:201	Thermoplastic starch is a potentially sustainable and biodegradable material.
34838853	0	37	theme	wax	15:17	arg1	Effect					0:5	Effect	0:5	Effect of palm wax on the mechanical, thermal, and moisture absorption properties of thermoplastic cassava starch composites.	0:124	Effect of palm wax on the mechanical, thermal, and moisture absorption properties of thermoplastic cassava starch composites.
34838853	11	38	theme	moisture	1512:1519	arg1	characteristics					1532:1546	improved mechanical and moisture resistance characteristics	1488:1546	improved mechanical and moisture resistance characteristics	1488:1546	Overall, the developed TPCS/palm wax with improved mechanical and moisture resistance characteristics has the potential to be used as biodegradable materials.
34838853	0	39	theme	starch	107:112	arg1	composites					114:123	thermoplastic cassava starch composites	85:123	thermoplastic cassava starch composites	85:123	Effect of palm wax on the mechanical, thermal, and moisture absorption properties of thermoplastic cassava starch composites.
34838853	8	40	theme	interaction	1164:1174	arg1	existence					1136:1144	the existence	1132:1144	the existence of intermolecular interaction between TPCS and palm wax with the intermolecular hydrogen bonds that existed between them	1132:1265	FT-IR indicated the existence of intermolecular interaction between TPCS and palm wax with the intermolecular hydrogen bonds that existed between them.
34838853	10	41	theme	absorption	1410:1419	arg1	value					1421:1425	the lowest moisture absorption value	1390:1425	the lowest moisture absorption value among the samples	1390:1443	The incorporation of 15 wt% palm wax resulted in the lowest moisture absorption value among the samples.
34838853	0	42	theme	palm	10:13	arg1	wax					15:17	palm wax	10:17	palm wax	10:17	Effect of palm wax on the mechanical, thermal, and moisture absorption properties of thermoplastic cassava starch composites.
34838853	0	43	theme	cassava	99:105	arg1	composites					114:123	thermoplastic cassava starch composites	85:123	thermoplastic cassava starch composites	85:123	Effect of palm wax on the mechanical, thermal, and moisture absorption properties of thermoplastic cassava starch composites.
34838853	2	44	theme	sensitivity	296:306	arg1	terms					246:250	terms	246:250	terms of mechanical performance and high moisture sensitivity	246:306	However, it possesses some limitations in terms of mechanical performance and high moisture sensitivity.
34838853	7	45	theme	samples	1030:1036	arg1	morphology					1012:1021	the morphology	1008:1021	the morphology of the samples	1008:1036	Besides, the morphology of the samples demonstrated irregular and rougher cleavage fracture after palm wax addition.
34838853	7	46	theme	wax	1102:1104	arg1	addition					1106:1113	palm wax addition	1097:1113	palm wax addition	1097:1113	Besides, the morphology of the samples demonstrated irregular and rougher cleavage fracture after palm wax addition.
34838853	6	47	theme	starch-based	877:888	arg1	thermoplastics					890:903	starch-based thermoplastics	877:903	starch-based thermoplastics	877:903	The findings showed that incorporating starch-based thermoplastics with palm wax has remarkably improved mechanical characteristics of the thermoplastic blends.
34838853	8	48	dep	TPCS	1184:1187	arg1	wax					1198:1200	wax	1198:1200	wax	1198:1200	FT-IR indicated the existence of intermolecular interaction between TPCS and palm wax with the intermolecular hydrogen bonds that existed between them.
34838853	6	49	theme	blends	991:996	arg1	characteristics					954:968	mechanical characteristics	943:968	mechanical characteristics of the thermoplastic blends	943:996	The findings showed that incorporating starch-based thermoplastics with palm wax has remarkably improved mechanical characteristics of the thermoplastic blends.
34838853	5	50	theme	differential	637:648	arg1	DSC					672:674	DSC	672:674	DSC	672:674	Next, characterization via scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), mechanical, water solubility, thickness swelling and moisture absorption tests, were conducted on the samples.
34838853	5	50	theme	differential	637:648	arg1	calorimetry					659:669	differential scanning calorimetry	637:669	differential scanning calorimetry (DSC)	637:675	Next, characterization via scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), mechanical, water solubility, thickness swelling and moisture absorption tests, were conducted on the samples.
34838853	8	51	theme	intermolecular	1149:1162	arg1	interaction					1164:1174	intermolecular interaction	1149:1174	intermolecular interaction between TPCS and palm wax with the intermolecular hydrogen bonds that existed between them	1149:1265	FT-IR indicated the existence of intermolecular interaction between TPCS and palm wax with the intermolecular hydrogen bonds that existed between them.
34838853	5	52	theme	thickness	757:765	arg1	swelling					767:774	thickness swelling	757:774	thickness swelling	757:774	Next, characterization via scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), mechanical, water solubility, thickness swelling and moisture absorption tests, were conducted on the samples.
34838853	4	53	theme	wax	510:512	arg1	ratios					495:500	the ratios	491:500	the ratios of palm wax (2.5, 5, 10, and 15 wt%)	491:537	TPCS was prepared via hot pressing by varying the ratios of palm wax (2.5, 5, 10, and 15 wt%).
34838853	0	54	theme	composites	114:123	arg1	thermal					38:44	thermal	38:44	thermal	38:44	Effect of palm wax on the mechanical, thermal, and moisture absorption properties of thermoplastic cassava starch composites.
34838853	3	55	theme	starch	376:381	arg1	characteristics					335:349	the characteristics	331:349	the characteristics of thermoplastic cassava starch (TPCS) containing palm wax at various loading	331:427	In this current work, the characteristics of thermoplastic cassava starch (TPCS) containing palm wax at various loading were evaluated.
34838853	8	56	with	interaction	1164:1174	arg1	bonds					1235:1239	the intermolecular hydrogen bonds	1207:1239	the intermolecular hydrogen bonds that existed between them	1207:1265	FT-IR indicated the existence of intermolecular interaction between TPCS and palm wax with the intermolecular hydrogen bonds that existed between them.
34838853	6	57	theme	thermoplastic	977:989	arg1	blends					991:996	the thermoplastic blends	973:996	the thermoplastic blends	973:996	The findings showed that incorporating starch-based thermoplastics with palm wax has remarkably improved mechanical characteristics of the thermoplastic blends.
34838853	4	58	theme	palm	505:508	arg1	%					536:536	2.5, 5, 10, and 15 wt%	515:536	2.5, 5, 10, and 15 wt%	515:536	TPCS was prepared via hot pressing by varying the ratios of palm wax (2.5, 5, 10, and 15 wt%).
34838853	4	58	theme	palm	505:508	arg1	wax					510:512	palm wax	505:512	palm wax (2.5, 5, 10, and 15 wt%)	505:537	TPCS was prepared via hot pressing by varying the ratios of palm wax (2.5, 5, 10, and 15 wt%).
34838853	11	59	theme	mechanical	1497:1506	arg1	characteristics					1532:1546	improved mechanical and moisture resistance characteristics	1488:1546	improved mechanical and moisture resistance characteristics	1488:1546	Overall, the developed TPCS/palm wax with improved mechanical and moisture resistance characteristics has the potential to be used as biodegradable materials.
34838853	3	60	theme	palm	401:404	arg1	wax					406:408	palm wax	401:408	palm wax	401:408	In this current work, the characteristics of thermoplastic cassava starch (TPCS) containing palm wax at various loading were evaluated.
34838853	5	61	theme	thermogravimetric	603:619	arg1	analysis					621:628	thermogravimetric analysis	603:628	thermogravimetric analysis (TGA)	603:634	Next, characterization via scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), mechanical, water solubility, thickness swelling and moisture absorption tests, were conducted on the samples.
34838853	5	61	theme	thermogravimetric	603:619	arg1	TGA					631:633	TGA	631:633	TGA	631:633	Next, characterization via scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), mechanical, water solubility, thickness swelling and moisture absorption tests, were conducted on the samples.
34838853	10	62	theme	%	1367:1367	arg1	wax					1374:1376	15 wt% palm wax	1362:1376	15 wt% palm wax	1362:1376	The incorporation of 15 wt% palm wax resulted in the lowest moisture absorption value among the samples.
34838853	2	63	theme	mechanical	255:264	arg1	performance					266:276	mechanical performance	255:276	mechanical performance	255:276	However, it possesses some limitations in terms of mechanical performance and high moisture sensitivity.
34838853	8	64	theme	intermolecular	1211:1224	arg1	bonds					1235:1239	the intermolecular hydrogen bonds	1207:1239	the intermolecular hydrogen bonds that existed between them	1207:1265	FT-IR indicated the existence of intermolecular interaction between TPCS and palm wax with the intermolecular hydrogen bonds that existed between them.
34838853	11	65	theme	resistance	1521:1530	arg1	characteristics					1532:1546	improved mechanical and moisture resistance characteristics	1488:1546	improved mechanical and moisture resistance characteristics	1488:1546	Overall, the developed TPCS/palm wax with improved mechanical and moisture resistance characteristics has the potential to be used as biodegradable materials.
34838853	11	66	with	wax	1479:1481	arg1	characteristics					1532:1546	improved mechanical and moisture resistance characteristics	1488:1546	improved mechanical and moisture resistance characteristics	1488:1546	Overall, the developed TPCS/palm wax with improved mechanical and moisture resistance characteristics has the potential to be used as biodegradable materials.
34838853	11	67	theme	biodegradable	1580:1592	arg1	materials					1594:1602	biodegradable materials	1580:1602	biodegradable materials	1580:1602	Overall, the developed TPCS/palm wax with improved mechanical and moisture resistance characteristics has the potential to be used as biodegradable materials.
34838853	9	68	theme	palm	1323:1326	arg1	content					1332:1338	palm wax content	1323:1338	palm wax content	1323:1338	The thermal stability of TPCS has improved with rising palm wax content.
34838853	2	69	contain	possesses	216:224	arg1	it					213:214	it	213:214	it	213:214	However, it possesses some limitations in terms of mechanical performance and high moisture sensitivity.
34838853	2	69	contain	possesses	216:224	arg2	limitations					231:241	some limitations	226:241	some limitations in terms of mechanical performance and high moisture sensitivity	226:306	However, it possesses some limitations in terms of mechanical performance and high moisture sensitivity.
34838853	11	70	theme	improved	1488:1495	arg1	characteristics					1532:1546	improved mechanical and moisture resistance characteristics	1488:1546	improved mechanical and moisture resistance characteristics	1488:1546	Overall, the developed TPCS/palm wax with improved mechanical and moisture resistance characteristics has the potential to be used as biodegradable materials.
34838853	1	71	theme	Thermoplastic	126:138	arg1	starch					140:145	Thermoplastic starch	126:145	Thermoplastic starch	126:145	Thermoplastic starch is a potentially sustainable and biodegradable material.
34838853	1	71	theme	Thermoplastic	126:138	arg1	material					194:201	a potentially sustainable and biodegradable material	150:201	a potentially sustainable and biodegradable material	150:201	Thermoplastic starch is a potentially sustainable and biodegradable material.
34838853	5	72	theme	moisture	780:787	arg1	tests					800:804	moisture absorption tests	780:804	moisture absorption tests	780:804	Next, characterization via scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), mechanical, water solubility, thickness swelling and moisture absorption tests, were conducted on the samples.
34838853	11	73	contain	has	1548:1550	arg2	potential					1556:1564	the potential to be used as biodegradable materials	1552:1602	the potential to be used as biodegradable materials	1552:1602	Overall, the developed TPCS/palm wax with improved mechanical and moisture resistance characteristics has the potential to be used as biodegradable materials.
34838853	11	73	contain	has	1548:1550	arg1	wax					1479:1481	the developed TPCS/palm wax	1455:1481	the developed TPCS/palm wax with improved mechanical and moisture resistance characteristics	1455:1546	Overall, the developed TPCS/palm wax with improved mechanical and moisture resistance characteristics has the potential to be used as biodegradable materials.
34838853	5	74	theme	water	739:743	arg1	solubility					745:754	water solubility	739:754	water solubility	739:754	Next, characterization via scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), mechanical, water solubility, thickness swelling and moisture absorption tests, were conducted on the samples.
34838853	11	75	theme	TPCS/palm	1469:1477	arg1	wax					1479:1481	the developed TPCS/palm wax	1455:1481	the developed TPCS/palm wax with improved mechanical and moisture resistance characteristics	1455:1546	Overall, the developed TPCS/palm wax with improved mechanical and moisture resistance characteristics has the potential to be used as biodegradable materials.
34838853	0	76	theme	absorption	60:69	arg1	properties					71:80	the mechanical, thermal, and moisture absorption properties	22:80	properties	71:80	Effect of palm wax on the mechanical, thermal, and moisture absorption properties of thermoplastic cassava starch composites.
32480094	8	0	theme	C-CS	1006:1009	arg1	compositions					1020:1031	All C-CS scaffold compositions	1002:1031	All C-CS scaffold compositions	1002:1031	All C-CS scaffold compositions supported PCa growth and the 4-1 scaffolds had the greatest cell numbers for both PC-3 and 22Rv1.
32480094	4	1	theme	PCa	559:561	arg1	culture					563:569	PCa culture	559:569	PCa culture	559:569	Three-dimensional porous chitosan-CS (C-CS) scaffolds were developed and evaluated for PCa culture.
32480094	5	2	theme	%	629:629	arg1	chitosan					631:638	4 w/v% chitosan	624:638	4 w/v% chitosan	624:638	Three C-CS scaffold compositions were prepared with 4 w/v% chitosan and 0.1, 0.5, and 1.0 w/v% CS and named 4-0.1, 4-0.5, and 4-1, respectively.
32480094	2	3	theme	tissue	314:319	arg1	matrix					288:293	the extracellular matrix	270:293	the extracellular matrix of normal prostate tissue and PCa	270:327	Chondroitin sulfate (CS) is found in the extracellular matrix of normal prostate tissue and PCa, with greater content in metastatic PCa.
32480094	6	4	theme	C-CS	721:724	arg1	scaffolds					726:734	The C-CS scaffolds	717:734	The C-CS scaffolds	717:734	The C-CS scaffolds had 90-95% porosity, average pore sizes between 143 and 166 μm, and no significant difference in scaffold stiffness.
32480094	8	5	theme	PCa	1043:1045	arg1	growth					1047:1052	PCa growth	1043:1052	PCa growth	1043:1052	All C-CS scaffold compositions supported PCa growth and the 4-1 scaffolds had the greatest cell numbers for both PC-3 and 22Rv1.
32480094	11	6	theme	drug	1540:1543	arg1	resistance					1545:1554	the greatest drug resistance	1527:1554	the greatest drug resistance	1527:1554	C-CS scaffolds promoted docetaxel drug resistance in PC-3 and 22Rv1 cultures and the 4-1 scaffold cultures had the greatest drug resistance.
32480094	10	7	theme	CS	1286:1287	arg1	concentration					1289:1301	Increasing CS concentration	1275:1301	Increasing CS concentration	1275:1301	Increasing CS concentration promoted upregulated vimentin expression in PC-3 cultures and N-cadherin and MMP-2 expression in 22Rv1 cultures.
32480094	12	8	theme	in	1616:1617	arg1	platform					1625:1632	a promising in vitro platform	1604:1632	a promising in vitro platform for PCa	1604:1640	These results indicate that C-CS scaffolds are a promising in vitro platform for PCa.
32480094	12	8	theme	in	1616:1617	arg1	scaffolds					1590:1598	C-CS scaffolds	1585:1598	C-CS scaffolds	1585:1598	These results indicate that C-CS scaffolds are a promising in vitro platform for PCa.
32480094	5	9	theme	4	624:624	arg1	w/v					626:628	w/v	626:628	w/v	626:628	Three C-CS scaffold compositions were prepared with 4 w/v% chitosan and 0.1, 0.5, and 1.0 w/v% CS and named 4-0.1, 4-0.5, and 4-1, respectively.
32480094	11	10	theme	docetaxel	1440:1448	arg1	resistance					1455:1464	docetaxel drug resistance	1440:1464	docetaxel drug resistance in PC-3 and 22Rv1 cultures	1440:1491	C-CS scaffolds promoted docetaxel drug resistance in PC-3 and 22Rv1 cultures and the 4-1 scaffold cultures had the greatest drug resistance.
32480094	8	11	theme	4-1	1062:1064	arg1	scaffolds					1066:1074	the 4-1 scaffolds	1058:1074	the 4-1 scaffolds	1058:1074	All C-CS scaffold compositions supported PCa growth and the 4-1 scaffolds had the greatest cell numbers for both PC-3 and 22Rv1.
32480094	6	12	from	sizes	770:774	arg1	stiffness					842:850	scaffold stiffness	833:850	scaffold stiffness	833:850	The C-CS scaffolds had 90-95% porosity, average pore sizes between 143 and 166 μm, and no significant difference in scaffold stiffness.
32480094	7	13	theme	PCa	943:945	arg1	growth					947:952	PCa growth	943:952	PCa growth	943:952	PC-3 and 22Rv1 PCa cells were cultured on the C-CS scaffolds to study the effect of CS on PCa growth and epithelial to mesenchymal transition (EMT).
32480094	7	14	theme	CS	937:938	arg1	effect					927:932	the effect	923:932	the effect of CS on PCa growth and epithelial to mesenchymal transition (EMT)	923:999	PC-3 and 22Rv1 PCa cells were cultured on the C-CS scaffolds to study the effect of CS on PCa growth and epithelial to mesenchymal transition (EMT).
32480094	7	15	theme	C-CS	899:902	arg1	scaffolds					904:912	the C-CS scaffolds	895:912	the C-CS scaffolds	895:912	PC-3 and 22Rv1 PCa cells were cultured on the C-CS scaffolds to study the effect of CS on PCa growth and epithelial to mesenchymal transition (EMT).
32480094	8	16	theme	greatest	1084:1091	arg1	numbers					1098:1104	the greatest cell numbers	1080:1104	the greatest cell numbers	1080:1104	All C-CS scaffold compositions supported PCa growth and the 4-1 scaffolds had the greatest cell numbers for both PC-3 and 22Rv1.
32480094	10	17	from	expression	1333:1342	arg1	cultures					1352:1359	PC-3 cultures	1347:1359	PC-3 cultures	1347:1359	Increasing CS concentration promoted upregulated vimentin expression in PC-3 cultures and N-cadherin and MMP-2 expression in 22Rv1 cultures.
32480094	10	17	from	expression	1333:1342	arg1	cultures					1406:1413	22Rv1 cultures	1400:1413	22Rv1 cultures	1400:1413	Increasing CS concentration promoted upregulated vimentin expression in PC-3 cultures and N-cadherin and MMP-2 expression in 22Rv1 cultures.
32480094	6	18	from	porosity	747:754	arg1	stiffness					842:850	scaffold stiffness	833:850	scaffold stiffness	833:850	The C-CS scaffolds had 90-95% porosity, average pore sizes between 143 and 166 μm, and no significant difference in scaffold stiffness.
32480094	8	19	contain	had	1076:1078	arg2	numbers					1098:1104	the greatest cell numbers	1080:1104	the greatest cell numbers	1080:1104	All C-CS scaffold compositions supported PCa growth and the 4-1 scaffolds had the greatest cell numbers for both PC-3 and 22Rv1.
32480094	8	19	contain	had	1076:1078	arg1	scaffolds					1066:1074	the 4-1 scaffolds	1058:1074	the 4-1 scaffolds	1058:1074	All C-CS scaffold compositions supported PCa growth and the 4-1 scaffolds had the greatest cell numbers for both PC-3 and 22Rv1.
32480094	5	20	dep	CS	667:668	arg1	w/v					662:664	w/v	662:664	w/v	662:664	Three C-CS scaffold compositions were prepared with 4 w/v% chitosan and 0.1, 0.5, and 1.0 w/v% CS and named 4-0.1, 4-0.5, and 4-1, respectively.
32480094	2	21	theme	Chondroitin	233:243	arg1	CS					254:255	CS	254:255	CS	254:255	Chondroitin sulfate (CS) is found in the extracellular matrix of normal prostate tissue and PCa, with greater content in metastatic PCa.
32480094	2	21	theme	Chondroitin	233:243	arg1	sulfate					245:251	Chondroitin sulfate	233:251	Chondroitin sulfate (CS)	233:256	Chondroitin sulfate (CS) is found in the extracellular matrix of normal prostate tissue and PCa, with greater content in metastatic PCa.
32480094	12	22	theme	promising	1606:1614	arg1	platform					1625:1632	a promising in vitro platform	1604:1632	a promising in vitro platform for PCa	1604:1640	These results indicate that C-CS scaffolds are a promising in vitro platform for PCa.
32480094	12	22	theme	promising	1606:1614	arg1	scaffolds					1590:1598	C-CS scaffolds	1585:1598	C-CS scaffolds	1585:1598	These results indicate that C-CS scaffolds are a promising in vitro platform for PCa.
32480094	5	23	dep	w/v	662:664	arg1	0.5					649:651	0.5	649:651	0.5	649:651	Three C-CS scaffold compositions were prepared with 4 w/v% chitosan and 0.1, 0.5, and 1.0 w/v% CS and named 4-0.1, 4-0.5, and 4-1, respectively.
32480094	5	23	dep	w/v	662:664	arg1	1.0					658:660	1.0	658:660	1.0	658:660	Three C-CS scaffold compositions were prepared with 4 w/v% chitosan and 0.1, 0.5, and 1.0 w/v% CS and named 4-0.1, 4-0.5, and 4-1, respectively.
32480094	12	24	dep	in	1616:1617	arg1	vitro					1619:1623	vitro	1619:1623	vitro	1619:1623	These results indicate that C-CS scaffolds are a promising in vitro platform for PCa.
32480094	0	25	theme	mesenchymal	71:81	arg1	transition					83:92	epithelial to mesenchymal transition	57:92	epithelial to mesenchymal transition	57:92	3D porous chitosan-chondroitin sulfate scaffolds promote epithelial to mesenchymal transition in prostate cancer cells.
32480094	7	26	theme	PCa	868:870	arg1	cells					872:876	PC-3 and 22Rv1 PCa cells	853:876	cells	872:876	PC-3 and 22Rv1 PCa cells were cultured on the C-CS scaffolds to study the effect of CS on PCa growth and epithelial to mesenchymal transition (EMT).
32480094	6	27	theme	scaffold	833:840	arg1	stiffness					842:850	scaffold stiffness	833:850	scaffold stiffness	833:850	The C-CS scaffolds had 90-95% porosity, average pore sizes between 143 and 166 μm, and no significant difference in scaffold stiffness.
32480094	10	28	theme	vimentin	1324:1331	arg1	expression					1333:1342	upregulated vimentin expression	1312:1342	upregulated vimentin expression in PC-3 cultures	1312:1359	Increasing CS concentration promoted upregulated vimentin expression in PC-3 cultures and N-cadherin and MMP-2 expression in 22Rv1 cultures.
32480094	12	29	theme	C-CS	1585:1588	arg1	platform					1625:1632	a promising in vitro platform	1604:1632	a promising in vitro platform for PCa	1604:1640	These results indicate that C-CS scaffolds are a promising in vitro platform for PCa.
32480094	12	29	theme	C-CS	1585:1588	arg1	scaffolds					1590:1598	C-CS scaffolds	1585:1598	C-CS scaffolds	1585:1598	These results indicate that C-CS scaffolds are a promising in vitro platform for PCa.
32480094	10	30	from	expression	1386:1395	arg1	cultures					1352:1359	PC-3 cultures	1347:1359	PC-3 cultures	1347:1359	Increasing CS concentration promoted upregulated vimentin expression in PC-3 cultures and N-cadherin and MMP-2 expression in 22Rv1 cultures.
32480094	10	30	from	expression	1386:1395	arg1	cultures					1406:1413	22Rv1 cultures	1400:1413	22Rv1 cultures	1400:1413	Increasing CS concentration promoted upregulated vimentin expression in PC-3 cultures and N-cadherin and MMP-2 expression in 22Rv1 cultures.
32480094	4	31	theme	porous	490:495	arg1	C-CS					510:513	C-CS	510:513	C-CS	510:513	Three-dimensional porous chitosan-CS (C-CS) scaffolds were developed and evaluated for PCa culture.
32480094	4	31	theme	porous	490:495	arg1	chitosan-CS					497:507	Three-dimensional porous chitosan-CS	472:507	Three-dimensional porous chitosan-CS (C-CS) scaffolds	472:524	Three-dimensional porous chitosan-CS (C-CS) scaffolds were developed and evaluated for PCa culture.
32480094	0	32	dep	mesenchymal	71:81	arg1	to					68:69	to	68:69	to	68:69	3D porous chitosan-chondroitin sulfate scaffolds promote epithelial to mesenchymal transition in prostate cancer cells.
32480094	0	33	theme	cancer	106:111	arg1	cells					113:117	prostate cancer cells	97:117	prostate cancer cells	97:117	3D porous chitosan-chondroitin sulfate scaffolds promote epithelial to mesenchymal transition in prostate cancer cells.
32480094	9	34	theme	upregulated	1159:1169	arg1	expression					1182:1191	upregulated EMT marker expression	1159:1191	upregulated EMT marker expression	1159:1191	The C-CS scaffolds promoted upregulated EMT marker expression compared to 2D cultures with the greatest EMT marker expression in 4-1 scaffolds.
32480094	5	35	theme	scaffold	583:590	arg1	compositions					592:603	Three C-CS scaffold compositions	572:603	Three C-CS scaffold compositions	572:603	Three C-CS scaffold compositions were prepared with 4 w/v% chitosan and 0.1, 0.5, and 1.0 w/v% CS and named 4-0.1, 4-0.5, and 4-1, respectively.
32480094	9	36	theme	marker	1175:1180	arg1	expression					1182:1191	upregulated EMT marker expression	1159:1191	upregulated EMT marker expression	1159:1191	The C-CS scaffolds promoted upregulated EMT marker expression compared to 2D cultures with the greatest EMT marker expression in 4-1 scaffolds.
32480094	2	37	theme	greater	335:341	arg1	content					343:349	greater content	335:349	greater content in metastatic PCa	335:367	Chondroitin sulfate (CS) is found in the extracellular matrix of normal prostate tissue and PCa, with greater content in metastatic PCa.
32480094	0	38	theme	3D	0:1	arg1	scaffolds					39:47	3D porous chitosan-chondroitin sulfate scaffolds	0:47	3D porous chitosan-chondroitin sulfate scaffolds	0:47	3D porous chitosan-chondroitin sulfate scaffolds promote epithelial to mesenchymal transition in prostate cancer cells.
32480094	11	39	contain	had	1523:1525	arg1	cultures					1514:1521	the 4-1 scaffold cultures	1497:1521	the 4-1 scaffold cultures	1497:1521	C-CS scaffolds promoted docetaxel drug resistance in PC-3 and 22Rv1 cultures and the 4-1 scaffold cultures had the greatest drug resistance.
32480094	11	39	contain	had	1523:1525	arg2	resistance					1545:1554	the greatest drug resistance	1527:1554	the greatest drug resistance	1527:1554	C-CS scaffolds promoted docetaxel drug resistance in PC-3 and 22Rv1 cultures and the 4-1 scaffold cultures had the greatest drug resistance.
32480094	10	40	theme	MMP-2	1380:1384	arg1	expression					1386:1395	N-cadherin and MMP-2 expression	1365:1395	expression	1386:1395	Increasing CS concentration promoted upregulated vimentin expression in PC-3 cultures and N-cadherin and MMP-2 expression in 22Rv1 cultures.
32480094	0	41	theme	chitosan-chondroitin	10:29	arg1	scaffolds					39:47	3D porous chitosan-chondroitin sulfate scaffolds	0:47	3D porous chitosan-chondroitin sulfate scaffolds	0:47	3D porous chitosan-chondroitin sulfate scaffolds promote epithelial to mesenchymal transition in prostate cancer cells.
32480094	9	42	theme	2D	1205:1206	arg1	cultures					1208:1215	2D cultures	1205:1215	2D cultures with the greatest EMT marker expression in 4-1 scaffolds	1205:1272	The C-CS scaffolds promoted upregulated EMT marker expression compared to 2D cultures with the greatest EMT marker expression in 4-1 scaffolds.
32480094	1	43	theme	Prostate	120:127	arg1	PCa					137:139	PCa	137:139	PCa	137:139	Prostate cancer (PCa) is a common cancer in men that is curable prior to metastasis, when its prognosis worsens.
32480094	1	43	theme	Prostate	120:127	arg1	cancer					129:134	Prostate cancer	120:134	Prostate cancer (PCa)	120:140	Prostate cancer (PCa) is a common cancer in men that is curable prior to metastasis, when its prognosis worsens.
32480094	1	43	theme	Prostate	120:127	arg1	curable					176:182	curable	176:182	curable	176:182	Prostate cancer (PCa) is a common cancer in men that is curable prior to metastasis, when its prognosis worsens.
32480094	1	43	theme	Prostate	120:127	arg1	cancer					154:159	a common cancer	145:159	a common cancer in men that is curable prior to metastasis, when its prognosis worsens	145:230	Prostate cancer (PCa) is a common cancer in men that is curable prior to metastasis, when its prognosis worsens.
32480094	10	44	theme	N-cadherin	1365:1374	arg1	expression					1386:1395	N-cadherin and MMP-2 expression	1365:1395	expression	1386:1395	Increasing CS concentration promoted upregulated vimentin expression in PC-3 cultures and N-cadherin and MMP-2 expression in 22Rv1 cultures.
32480094	6	45	theme	pore	765:768	arg1	sizes					770:774	average pore sizes	757:774	average pore sizes between 143 and 166 μm	757:797	The C-CS scaffolds had 90-95% porosity, average pore sizes between 143 and 166 μm, and no significant difference in scaffold stiffness.
32480094	3	46	theme	microenvironment	441:456	arg1	applications					458:469	tumor microenvironment applications	435:469	tumor microenvironment applications	435:469	Biomaterial scaffolds containing CS have yet to be evaluated for tumor microenvironment applications.
32480094	9	47	theme	greatest	1226:1233	arg1	EMT					1235:1237	the greatest EMT	1222:1237	the greatest EMT marker expression in 4-1 scaffolds	1222:1272	The C-CS scaffolds promoted upregulated EMT marker expression compared to 2D cultures with the greatest EMT marker expression in 4-1 scaffolds.
32480094	9	48	theme	marker	1239:1244	arg1	expression					1246:1255	the greatest EMT marker expression	1222:1255	the greatest EMT marker expression in 4-1 scaffolds	1222:1272	The C-CS scaffolds promoted upregulated EMT marker expression compared to 2D cultures with the greatest EMT marker expression in 4-1 scaffolds.
32480094	9	49	from	expression	1246:1255	arg1	scaffolds					1264:1272	4-1 scaffolds	1260:1272	4-1 scaffolds	1260:1272	The C-CS scaffolds promoted upregulated EMT marker expression compared to 2D cultures with the greatest EMT marker expression in 4-1 scaffolds.
32480094	6	50	theme	%	745:745	arg1	porosity					747:754	90-95% porosity	740:754	90-95% porosity	740:754	The C-CS scaffolds had 90-95% porosity, average pore sizes between 143 and 166 μm, and no significant difference in scaffold stiffness.
32480094	11	51	theme	scaffold	1505:1512	arg1	cultures					1514:1521	the 4-1 scaffold cultures	1497:1521	the 4-1 scaffold cultures	1497:1521	C-CS scaffolds promoted docetaxel drug resistance in PC-3 and 22Rv1 cultures and the 4-1 scaffold cultures had the greatest drug resistance.
32480094	6	52	theme	significant	807:817	arg1	difference					819:828	no significant difference	804:828	no significant difference in scaffold stiffness	804:850	The C-CS scaffolds had 90-95% porosity, average pore sizes between 143 and 166 μm, and no significant difference in scaffold stiffness.
32480094	7	53	from	effect	927:932	arg1	transition					984:993	epithelial to mesenchymal transition	958:993	epithelial to mesenchymal transition (EMT)	958:999	PC-3 and 22Rv1 PCa cells were cultured on the C-CS scaffolds to study the effect of CS on PCa growth and epithelial to mesenchymal transition (EMT).
32480094	7	53	from	effect	927:932	arg1	growth					947:952	PCa growth	943:952	PCa growth	943:952	PC-3 and 22Rv1 PCa cells were cultured on the C-CS scaffolds to study the effect of CS on PCa growth and epithelial to mesenchymal transition (EMT).
32480094	7	53	from	effect	927:932	arg1	EMT					996:998	EMT	996:998	EMT	996:998	PC-3 and 22Rv1 PCa cells were cultured on the C-CS scaffolds to study the effect of CS on PCa growth and epithelial to mesenchymal transition (EMT).
32480094	5	54	theme	C-CS	578:581	arg1	compositions					592:603	Three C-CS scaffold compositions	572:603	Three C-CS scaffold compositions	572:603	Three C-CS scaffold compositions were prepared with 4 w/v% chitosan and 0.1, 0.5, and 1.0 w/v% CS and named 4-0.1, 4-0.5, and 4-1, respectively.
32480094	11	55	theme	greatest	1531:1538	arg1	resistance					1545:1554	the greatest drug resistance	1527:1554	the greatest drug resistance	1527:1554	C-CS scaffolds promoted docetaxel drug resistance in PC-3 and 22Rv1 cultures and the 4-1 scaffold cultures had the greatest drug resistance.
32480094	5	56	theme	w/v	626:628	arg1	chitosan					631:638	4 w/v% chitosan	624:638	4 w/v% chitosan	624:638	Three C-CS scaffold compositions were prepared with 4 w/v% chitosan and 0.1, 0.5, and 1.0 w/v% CS and named 4-0.1, 4-0.5, and 4-1, respectively.
32480094	10	57	theme	Increasing	1275:1284	arg1	concentration					1289:1301	Increasing CS concentration	1275:1301	Increasing CS concentration	1275:1301	Increasing CS concentration promoted upregulated vimentin expression in PC-3 cultures and N-cadherin and MMP-2 expression in 22Rv1 cultures.
32480094	7	58	theme	mesenchymal	972:982	arg1	transition					984:993	epithelial to mesenchymal transition	958:993	epithelial to mesenchymal transition (EMT)	958:999	PC-3 and 22Rv1 PCa cells were cultured on the C-CS scaffolds to study the effect of CS on PCa growth and epithelial to mesenchymal transition (EMT).
32480094	7	58	theme	mesenchymal	972:982	arg1	EMT					996:998	EMT	996:998	EMT	996:998	PC-3 and 22Rv1 PCa cells were cultured on the C-CS scaffolds to study the effect of CS on PCa growth and epithelial to mesenchymal transition (EMT).
32480094	8	59	theme	scaffold	1011:1018	arg1	compositions					1020:1031	All C-CS scaffold compositions	1002:1031	All C-CS scaffold compositions	1002:1031	All C-CS scaffold compositions supported PCa growth and the 4-1 scaffolds had the greatest cell numbers for both PC-3 and 22Rv1.
32480094	2	60	theme	prostate	305:312	arg1	tissue					314:319	normal prostate tissue	298:319	normal prostate tissue	298:319	Chondroitin sulfate (CS) is found in the extracellular matrix of normal prostate tissue and PCa, with greater content in metastatic PCa.
32480094	11	61	theme	C-CS	1416:1419	arg1	scaffolds					1421:1429	C-CS scaffolds	1416:1429	C-CS scaffolds	1416:1429	C-CS scaffolds promoted docetaxel drug resistance in PC-3 and 22Rv1 cultures and the 4-1 scaffold cultures had the greatest drug resistance.
32480094	8	62	theme	cell	1093:1096	arg1	numbers					1098:1104	the greatest cell numbers	1080:1104	the greatest cell numbers	1080:1104	All C-CS scaffold compositions supported PCa growth and the 4-1 scaffolds had the greatest cell numbers for both PC-3 and 22Rv1.
32480094	7	63	dep	mesenchymal	972:982	arg1	to					969:970	to	969:970	to	969:970	PC-3 and 22Rv1 PCa cells were cultured on the C-CS scaffolds to study the effect of CS on PCa growth and epithelial to mesenchymal transition (EMT).
32480094	9	64	with	cultures	1208:1215	arg1	expression					1246:1255	the greatest EMT marker expression	1222:1255	the greatest EMT marker expression in 4-1 scaffolds	1222:1272	The C-CS scaffolds promoted upregulated EMT marker expression compared to 2D cultures with the greatest EMT marker expression in 4-1 scaffolds.
32480094	2	65	theme	extracellular	274:286	arg1	matrix					288:293	the extracellular matrix	270:293	the extracellular matrix of normal prostate tissue and PCa	270:327	Chondroitin sulfate (CS) is found in the extracellular matrix of normal prostate tissue and PCa, with greater content in metastatic PCa.
32480094	10	66	theme	22Rv1	1400:1404	arg1	cultures					1406:1413	22Rv1 cultures	1400:1413	22Rv1 cultures	1400:1413	Increasing CS concentration promoted upregulated vimentin expression in PC-3 cultures and N-cadherin and MMP-2 expression in 22Rv1 cultures.
32480094	11	67	theme	drug	1450:1453	arg1	resistance					1455:1464	docetaxel drug resistance	1440:1464	docetaxel drug resistance in PC-3 and 22Rv1 cultures	1440:1491	C-CS scaffolds promoted docetaxel drug resistance in PC-3 and 22Rv1 cultures and the 4-1 scaffold cultures had the greatest drug resistance.
32480094	3	68	contain	containing	392:401	arg1	scaffolds					382:390	Biomaterial scaffolds	370:390	Biomaterial scaffolds containing CS	370:404	Biomaterial scaffolds containing CS have yet to be evaluated for tumor microenvironment applications.
32480094	3	68	contain	containing	392:401	arg2	CS					403:404	CS	403:404	CS	403:404	Biomaterial scaffolds containing CS have yet to be evaluated for tumor microenvironment applications.
32480094	1	69	from	cancer	154:159	arg1	men					164:166	men	164:166	men	164:166	Prostate cancer (PCa) is a common cancer in men that is curable prior to metastasis, when its prognosis worsens.
32480094	11	70	from	resistance	1455:1464	arg1	cultures					1484:1491	PC-3 and 22Rv1 cultures	1469:1491	cultures	1484:1491	C-CS scaffolds promoted docetaxel drug resistance in PC-3 and 22Rv1 cultures and the 4-1 scaffold cultures had the greatest drug resistance.
32480094	4	71	theme	Three-dimensional	472:488	arg1	C-CS					510:513	C-CS	510:513	C-CS	510:513	Three-dimensional porous chitosan-CS (C-CS) scaffolds were developed and evaluated for PCa culture.
32480094	4	71	theme	Three-dimensional	472:488	arg1	chitosan-CS					497:507	Three-dimensional porous chitosan-CS	472:507	Three-dimensional porous chitosan-CS (C-CS) scaffolds	472:524	Three-dimensional porous chitosan-CS (C-CS) scaffolds were developed and evaluated for PCa culture.
32480094	0	72	theme	prostate	97:104	arg1	cells					113:117	prostate cancer cells	97:117	prostate cancer cells	97:117	3D porous chitosan-chondroitin sulfate scaffolds promote epithelial to mesenchymal transition in prostate cancer cells.
32480094	9	73	theme	C-CS	1135:1138	arg1	scaffolds					1140:1148	The C-CS scaffolds	1131:1148	The C-CS scaffolds	1131:1148	The C-CS scaffolds promoted upregulated EMT marker expression compared to 2D cultures with the greatest EMT marker expression in 4-1 scaffolds.
32480094	1	74	theme	common	147:152	arg1	cancer					129:134	Prostate cancer	120:134	Prostate cancer (PCa)	120:140	Prostate cancer (PCa) is a common cancer in men that is curable prior to metastasis, when its prognosis worsens.
32480094	1	74	theme	common	147:152	arg1	curable					176:182	curable	176:182	curable	176:182	Prostate cancer (PCa) is a common cancer in men that is curable prior to metastasis, when its prognosis worsens.
32480094	1	74	theme	common	147:152	arg1	cancer					154:159	a common cancer	145:159	a common cancer in men that is curable prior to metastasis, when its prognosis worsens	145:230	Prostate cancer (PCa) is a common cancer in men that is curable prior to metastasis, when its prognosis worsens.
32480094	6	75	contain	had	736:738	arg2	difference					819:828	no significant difference	804:828	no significant difference in scaffold stiffness	804:850	The C-CS scaffolds had 90-95% porosity, average pore sizes between 143 and 166 μm, and no significant difference in scaffold stiffness.
32480094	6	75	contain	had	736:738	arg2	sizes					770:774	average pore sizes	757:774	average pore sizes between 143 and 166 μm	757:797	The C-CS scaffolds had 90-95% porosity, average pore sizes between 143 and 166 μm, and no significant difference in scaffold stiffness.
32480094	6	75	contain	had	736:738	arg1	scaffolds					726:734	The C-CS scaffolds	717:734	The C-CS scaffolds	717:734	The C-CS scaffolds had 90-95% porosity, average pore sizes between 143 and 166 μm, and no significant difference in scaffold stiffness.
32480094	6	75	contain	had	736:738	arg2	porosity					747:754	90-95% porosity	740:754	90-95% porosity	740:754	The C-CS scaffolds had 90-95% porosity, average pore sizes between 143 and 166 μm, and no significant difference in scaffold stiffness.
32480094	4	76	theme	chitosan-CS	497:507	arg1	scaffolds					516:524	Three-dimensional porous chitosan-CS (C-CS) scaffolds	472:524	Three-dimensional porous chitosan-CS (C-CS) scaffolds	472:524	Three-dimensional porous chitosan-CS (C-CS) scaffolds were developed and evaluated for PCa culture.
32480094	10	77	theme	upregulated	1312:1322	arg1	expression					1333:1342	upregulated vimentin expression	1312:1342	upregulated vimentin expression in PC-3 cultures	1312:1359	Increasing CS concentration promoted upregulated vimentin expression in PC-3 cultures and N-cadherin and MMP-2 expression in 22Rv1 cultures.
32480094	7	78	theme	22Rv1	862:866	arg1	cells					872:876	PC-3 and 22Rv1 PCa cells	853:876	cells	872:876	PC-3 and 22Rv1 PCa cells were cultured on the C-CS scaffolds to study the effect of CS on PCa growth and epithelial to mesenchymal transition (EMT).
32480094	9	79	theme	EMT	1171:1173	arg1	expression					1182:1191	upregulated EMT marker expression	1159:1191	upregulated EMT marker expression	1159:1191	The C-CS scaffolds promoted upregulated EMT marker expression compared to 2D cultures with the greatest EMT marker expression in 4-1 scaffolds.
32480094	7	80	theme	PC-3	853:856	arg1	cells					872:876	PC-3 and 22Rv1 PCa cells	853:876	cells	872:876	PC-3 and 22Rv1 PCa cells were cultured on the C-CS scaffolds to study the effect of CS on PCa growth and epithelial to mesenchymal transition (EMT).
32480094	10	81	theme	PC-3	1347:1350	arg1	cultures					1352:1359	PC-3 cultures	1347:1359	PC-3 cultures	1347:1359	Increasing CS concentration promoted upregulated vimentin expression in PC-3 cultures and N-cadherin and MMP-2 expression in 22Rv1 cultures.
32480094	0	82	theme	porous	3:8	arg1	scaffolds					39:47	3D porous chitosan-chondroitin sulfate scaffolds	0:47	3D porous chitosan-chondroitin sulfate scaffolds	0:47	3D porous chitosan-chondroitin sulfate scaffolds promote epithelial to mesenchymal transition in prostate cancer cells.
32480094	0	83	theme	sulfate	31:37	arg1	scaffolds					39:47	3D porous chitosan-chondroitin sulfate scaffolds	0:47	3D porous chitosan-chondroitin sulfate scaffolds	0:47	3D porous chitosan-chondroitin sulfate scaffolds promote epithelial to mesenchymal transition in prostate cancer cells.
32480094	2	84	located	found	261:265	arg2	sulfate					245:251	Chondroitin sulfate	233:251	Chondroitin sulfate (CS)	233:256	Chondroitin sulfate (CS) is found in the extracellular matrix of normal prostate tissue and PCa, with greater content in metastatic PCa.
32480094	2	84	located	found	261:265	arg1	matrix					288:293	the extracellular matrix	270:293	the extracellular matrix of normal prostate tissue and PCa	270:327	Chondroitin sulfate (CS) is found in the extracellular matrix of normal prostate tissue and PCa, with greater content in metastatic PCa.
32480094	2	84	located	found	261:265	arg2	CS					254:255	CS	254:255	CS	254:255	Chondroitin sulfate (CS) is found in the extracellular matrix of normal prostate tissue and PCa, with greater content in metastatic PCa.
32480094	3	85	theme	Biomaterial	370:380	arg1	scaffolds					382:390	Biomaterial scaffolds	370:390	Biomaterial scaffolds containing CS	370:404	Biomaterial scaffolds containing CS have yet to be evaluated for tumor microenvironment applications.
32480094	11	86	theme	22Rv1	1478:1482	arg1	cultures					1484:1491	PC-3 and 22Rv1 cultures	1469:1491	cultures	1484:1491	C-CS scaffolds promoted docetaxel drug resistance in PC-3 and 22Rv1 cultures and the 4-1 scaffold cultures had the greatest drug resistance.
32480094	2	87	theme	metastatic	354:363	arg1	PCa					365:367	metastatic PCa	354:367	metastatic PCa	354:367	Chondroitin sulfate (CS) is found in the extracellular matrix of normal prostate tissue and PCa, with greater content in metastatic PCa.
32480094	6	88	theme	average	757:763	arg1	sizes					770:774	average pore sizes	757:774	average pore sizes between 143 and 166 μm	757:797	The C-CS scaffolds had 90-95% porosity, average pore sizes between 143 and 166 μm, and no significant difference in scaffold stiffness.
32480094	5	89	theme	%	665:665	arg1	CS					667:668	0.1, 0.5, and 1.0 w/v% CS	644:668	0.1, 0.5, and 1.0 w/v% CS	644:668	Three C-CS scaffold compositions were prepared with 4 w/v% chitosan and 0.1, 0.5, and 1.0 w/v% CS and named 4-0.1, 4-0.5, and 4-1, respectively.
32480094	6	90	from	difference	819:828	arg1	stiffness					842:850	scaffold stiffness	833:850	scaffold stiffness	833:850	The C-CS scaffolds had 90-95% porosity, average pore sizes between 143 and 166 μm, and no significant difference in scaffold stiffness.
32480094	11	91	theme	PC-3	1469:1472	arg1	cultures					1484:1491	PC-3 and 22Rv1 cultures	1469:1491	cultures	1484:1491	C-CS scaffolds promoted docetaxel drug resistance in PC-3 and 22Rv1 cultures and the 4-1 scaffold cultures had the greatest drug resistance.
32480094	2	92	theme	normal	298:303	arg1	tissue					314:319	normal prostate tissue	298:319	normal prostate tissue	298:319	Chondroitin sulfate (CS) is found in the extracellular matrix of normal prostate tissue and PCa, with greater content in metastatic PCa.
32480094	9	93	theme	EMT	1235:1237	arg1	expression					1246:1255	the greatest EMT marker expression	1222:1255	the greatest EMT marker expression in 4-1 scaffolds	1222:1272	The C-CS scaffolds promoted upregulated EMT marker expression compared to 2D cultures with the greatest EMT marker expression in 4-1 scaffolds.
32480094	2	94	from	content	343:349	arg1	PCa					365:367	metastatic PCa	354:367	metastatic PCa	354:367	Chondroitin sulfate (CS) is found in the extracellular matrix of normal prostate tissue and PCa, with greater content in metastatic PCa.
32480094	11	95	theme	4-1	1501:1503	arg1	cultures					1514:1521	the 4-1 scaffold cultures	1497:1521	the 4-1 scaffold cultures	1497:1521	C-CS scaffolds promoted docetaxel drug resistance in PC-3 and 22Rv1 cultures and the 4-1 scaffold cultures had the greatest drug resistance.
32480094	3	96	theme	tumor	435:439	arg1	applications					458:469	tumor microenvironment applications	435:469	tumor microenvironment applications	435:469	Biomaterial scaffolds containing CS have yet to be evaluated for tumor microenvironment applications.
32480094	6	97	theme	90-95	740:744	arg1	%					745:745	%	745:745	%	745:745	The C-CS scaffolds had 90-95% porosity, average pore sizes between 143 and 166 μm, and no significant difference in scaffold stiffness.
32480094	2	98	theme	PCa	325:327	arg1	matrix					288:293	the extracellular matrix	270:293	the extracellular matrix of normal prostate tissue and PCa	270:327	Chondroitin sulfate (CS) is found in the extracellular matrix of normal prostate tissue and PCa, with greater content in metastatic PCa.
32480094	9	99	theme	4-1	1260:1262	arg1	scaffolds					1264:1272	4-1 scaffolds	1260:1272	4-1 scaffolds	1260:1272	The C-CS scaffolds promoted upregulated EMT marker expression compared to 2D cultures with the greatest EMT marker expression in 4-1 scaffolds.
33990962	1	0	used	used	335:338	arg2	method					324:329	then the hot-press method	305:329	then the hot-press method	305:329	BACKGROUND Different thermoplastic starch (TPS) films were prepared with or without the addition of microcrystalline cellulose (MCC) obtained via the melt-extrusion method, and then the hot-press method was used to produce environmentally friendly TPS-based film/paper composites to replace petroleum-based materials.
33990962	6	1	theme	composites	1095:1104	arg1	higher					1136:1141	higher	1136:1141	higher	1136:1141	In particular, it was found that the water contact angle of MCC@TPS/paper composites and TPS/paper composites were higher than single-layer paper.
33990962	6	1	theme	composites	1095:1104	arg1	angle					1072:1076	the water contact angle	1054:1076	the water contact angle of MCC@TPS/paper composites and TPS/paper composites	1054:1129	In particular, it was found that the water contact angle of MCC@TPS/paper composites and TPS/paper composites were higher than single-layer paper.
33990962	4	2	theme	water	809:813	arg1	rate					834:837	the water vapor transmission rate	805:837	the water vapor transmission rate of MCC@TPS/paper composites	805:865	% MCC was added to reinforce the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites.
33990962	1	3	theme	melt-extrusion	278:291	arg1	method					293:298	the melt-extrusion method	274:298	the melt-extrusion method	274:298	BACKGROUND Different thermoplastic starch (TPS) films were prepared with or without the addition of microcrystalline cellulose (MCC) obtained via the melt-extrusion method, and then the hot-press method was used to produce environmentally friendly TPS-based film/paper composites to replace petroleum-based materials.
33990962	7	4	from	requirement	1277:1287	arg1	industry					1359:1366	the food packaging industry	1340:1366	the food packaging industry	1340:1366	Furthermore, MCC reinforced paper-plastic composites demonstrated good barrier properties which can meet the requirement of the need for lower water sensitive materials in the food packaging industry.
33990962	4	5	theme	films	648:652	arg1	properties					630:639	the mechanical properties	615:639	the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites	615:865	% MCC was added to reinforce the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites.
33990962	5	6	theme	MCC	893:895	arg1	composites					907:916	MCC@TPS/paper composites	893:916	MCC@TPS/paper composites	893:916	TPS/paper composites and MCC@TPS/paper composites have better physical properties (i.e. smoothness, flexibility and folding resistance) than only paper.
33990962	4	7	theme	@	711:711	arg1	composites					722:731	MCC@TPS/paper composites	708:731	MCC@TPS/paper composites	708:731	% MCC was added to reinforce the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites.
33990962	5	8	theme	TPS/paper	897:905	arg1	composites					907:916	MCC@TPS/paper composites	893:916	MCC@TPS/paper composites	893:916	TPS/paper composites and MCC@TPS/paper composites have better physical properties (i.e. smoothness, flexibility and folding resistance) than only paper.
33990962	8	9	theme	film/paper	1412:1421	arg1	composites					1423:1432	CONCLUSION Thermoplastic corn starch-based film/paper composites	1369:1432	CONCLUSION Thermoplastic corn starch-based film/paper composites	1369:1432	CONCLUSION Thermoplastic corn starch-based film/paper composites have good application properties as a potential source of bioplastic materials.
33990962	1	10	theme	petroleum-based	419:433	arg1	materials					435:443	petroleum-based materials	419:443	petroleum-based materials	419:443	BACKGROUND Different thermoplastic starch (TPS) films were prepared with or without the addition of microcrystalline cellulose (MCC) obtained via the melt-extrusion method, and then the hot-press method was used to produce environmentally friendly TPS-based film/paper composites to replace petroleum-based materials.
33990962	1	11	theme	microcrystalline	228:243	arg1	MCC					256:258	MCC	256:258	MCC	256:258	BACKGROUND Different thermoplastic starch (TPS) films were prepared with or without the addition of microcrystalline cellulose (MCC) obtained via the melt-extrusion method, and then the hot-press method was used to produce environmentally friendly TPS-based film/paper composites to replace petroleum-based materials.
33990962	1	11	theme	microcrystalline	228:243	arg1	cellulose					245:253	microcrystalline cellulose	228:253	microcrystalline cellulose (MCC) obtained via the melt-extrusion method	228:298	BACKGROUND Different thermoplastic starch (TPS) films were prepared with or without the addition of microcrystalline cellulose (MCC) obtained via the melt-extrusion method, and then the hot-press method was used to produce environmentally friendly TPS-based film/paper composites to replace petroleum-based materials.
33990962	8	12	theme	materials	1503:1511	arg1	source					1482:1487	a potential source	1470:1487	a potential source of bioplastic materials	1470:1511	CONCLUSION Thermoplastic corn starch-based film/paper composites have good application properties as a potential source of bioplastic materials.
33990962	8	12	theme	materials	1503:1511	arg1	properties					1456:1465	good application properties	1439:1465	good application properties	1439:1465	CONCLUSION Thermoplastic corn starch-based film/paper composites have good application properties as a potential source of bioplastic materials.
33990962	4	13	theme	barrier	686:692	arg1	properties					694:703	the barrier properties	682:703	the barrier properties of MCC@TPS/paper composites	682:731	% MCC was added to reinforce the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites.
33990962	4	14	theme	water	758:762	arg1	vapor					764:768	water vapor	758:768	water vapor	758:768	% MCC was added to reinforce the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites.
33990962	8	15	contain	have	1434:1437	arg1	composites					1423:1432	CONCLUSION Thermoplastic corn starch-based film/paper composites	1369:1432	CONCLUSION Thermoplastic corn starch-based film/paper composites	1369:1432	CONCLUSION Thermoplastic corn starch-based film/paper composites have good application properties as a potential source of bioplastic materials.
33990962	8	15	contain	have	1434:1437	arg2	source					1482:1487	a potential source	1470:1487	a potential source of bioplastic materials	1470:1511	CONCLUSION Thermoplastic corn starch-based film/paper composites have good application properties as a potential source of bioplastic materials.
33990962	8	15	contain	have	1434:1437	arg2	properties					1456:1465	good application properties	1439:1465	good application properties	1439:1465	CONCLUSION Thermoplastic corn starch-based film/paper composites have good application properties as a potential source of bioplastic materials.
33990962	4	16	theme	composites	722:731	arg1	properties					694:703	the barrier properties	682:703	the barrier properties of MCC@TPS/paper composites	682:731	% MCC was added to reinforce the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites.
33990962	0	17	theme	composites	78:87	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Processing and characterization of thermoplastic corn starch-based film/paper composites containing microcrystalline cellulose.
33990962	0	17	theme	composites	78:87	arg1	Processing					0:9	Processing	0:9	Processing	0:9	Processing and characterization of thermoplastic corn starch-based film/paper composites containing microcrystalline cellulose.
33990962	0	18	theme	microcrystalline	100:115	arg1	cellulose					117:125	microcrystalline cellulose	100:125	microcrystalline cellulose	100:125	Processing and characterization of thermoplastic corn starch-based film/paper composites containing microcrystalline cellulose.
33990962	1	19	theme	friendly	367:374	arg1	composites					397:406	environmentally friendly TPS-based film/paper composites	351:406	environmentally friendly TPS-based film/paper composites	351:406	BACKGROUND Different thermoplastic starch (TPS) films were prepared with or without the addition of microcrystalline cellulose (MCC) obtained via the melt-extrusion method, and then the hot-press method was used to produce environmentally friendly TPS-based film/paper composites to replace petroleum-based materials.
33990962	5	20	theme	physical	930:937	arg1	properties					939:948	better physical properties	923:948	better physical properties (i.e. smoothness, flexibility and folding resistance)	923:1002	TPS/paper composites and MCC@TPS/paper composites have better physical properties (i.e. smoothness, flexibility and folding resistance) than only paper.
33990962	8	21	theme	Thermoplastic	1380:1392	arg1	composites					1423:1432	CONCLUSION Thermoplastic corn starch-based film/paper composites	1369:1432	CONCLUSION Thermoplastic corn starch-based film/paper composites	1369:1432	CONCLUSION Thermoplastic corn starch-based film/paper composites have good application properties as a potential source of bioplastic materials.
33990962	1	22	theme	thermoplastic	149:161	arg1	TPS					171:173	TPS	171:173	TPS	171:173	BACKGROUND Different thermoplastic starch (TPS) films were prepared with or without the addition of microcrystalline cellulose (MCC) obtained via the melt-extrusion method, and then the hot-press method was used to produce environmentally friendly TPS-based film/paper composites to replace petroleum-based materials.
33990962	1	22	theme	thermoplastic	149:161	arg1	starch					163:168	BACKGROUND Different thermoplastic starch	128:168	BACKGROUND Different thermoplastic starch (TPS) films	128:180	BACKGROUND Different thermoplastic starch (TPS) films were prepared with or without the addition of microcrystalline cellulose (MCC) obtained via the melt-extrusion method, and then the hot-press method was used to produce environmentally friendly TPS-based film/paper composites to replace petroleum-based materials.
33990962	7	23	theme	barrier	1239:1245	arg1	properties					1247:1256	good barrier properties	1234:1256	good barrier properties which can meet the requirement of the need for lower water sensitive materials in the food packaging industry	1234:1366	Furthermore, MCC reinforced paper-plastic composites demonstrated good barrier properties which can meet the requirement of the need for lower water sensitive materials in the food packaging industry.
33990962	1	24	theme	film/paper	386:395	arg1	composites					397:406	environmentally friendly TPS-based film/paper composites	351:406	environmentally friendly TPS-based film/paper composites	351:406	BACKGROUND Different thermoplastic starch (TPS) films were prepared with or without the addition of microcrystalline cellulose (MCC) obtained via the melt-extrusion method, and then the hot-press method was used to produce environmentally friendly TPS-based film/paper composites to replace petroleum-based materials.
33990962	8	25	theme	starch-based	1399:1410	arg1	composites					1423:1432	CONCLUSION Thermoplastic corn starch-based film/paper composites	1369:1432	CONCLUSION Thermoplastic corn starch-based film/paper composites	1369:1432	CONCLUSION Thermoplastic corn starch-based film/paper composites have good application properties as a potential source of bioplastic materials.
33990962	5	26	theme	only	1009:1012	arg1	paper					1014:1018	only paper	1009:1018	only paper	1009:1018	TPS/paper composites and MCC@TPS/paper composites have better physical properties (i.e. smoothness, flexibility and folding resistance) than only paper.
33990962	6	27	theme	MCC	1081:1083	arg1	composites					1095:1104	MCC@TPS/paper composites	1081:1104	MCC@TPS/paper composites	1081:1104	In particular, it was found that the water contact angle of MCC@TPS/paper composites and TPS/paper composites were higher than single-layer paper.
33990962	7	28	theme	paper-plastic	1196:1208	arg1	composites					1210:1219	MCC reinforced paper-plastic composites	1181:1219	MCC reinforced paper-plastic composites	1181:1219	Furthermore, MCC reinforced paper-plastic composites demonstrated good barrier properties which can meet the requirement of the need for lower water sensitive materials in the food packaging industry.
33990962	1	29	theme	BACKGROUND	128:137	arg1	TPS					171:173	TPS	171:173	TPS	171:173	BACKGROUND Different thermoplastic starch (TPS) films were prepared with or without the addition of microcrystalline cellulose (MCC) obtained via the melt-extrusion method, and then the hot-press method was used to produce environmentally friendly TPS-based film/paper composites to replace petroleum-based materials.
33990962	1	29	theme	BACKGROUND	128:137	arg1	starch					163:168	BACKGROUND Different thermoplastic starch	128:168	BACKGROUND Different thermoplastic starch (TPS) films	128:180	BACKGROUND Different thermoplastic starch (TPS) films were prepared with or without the addition of microcrystalline cellulose (MCC) obtained via the melt-extrusion method, and then the hot-press method was used to produce environmentally friendly TPS-based film/paper composites to replace petroleum-based materials.
33990962	7	30	theme	packaging	1349:1357	arg1	industry					1359:1366	the food packaging industry	1340:1366	the food packaging industry	1340:1366	Furthermore, MCC reinforced paper-plastic composites demonstrated good barrier properties which can meet the requirement of the need for lower water sensitive materials in the food packaging industry.
33990962	2	31	theme	interfacial	498:508	arg1	adhesion					510:517	good interfacial adhesion	493:517	good interfacial adhesion	493:517	RESULTS The paper-plastic composites exhibited good interfacial adhesion from the scannign elctron microscopy images.
33990962	8	32	theme	good	1439:1442	arg1	source					1482:1487	a potential source	1470:1487	a potential source of bioplastic materials	1470:1511	CONCLUSION Thermoplastic corn starch-based film/paper composites have good application properties as a potential source of bioplastic materials.
33990962	8	32	theme	good	1439:1442	arg1	properties					1456:1465	good application properties	1439:1465	good application properties	1439:1465	CONCLUSION Thermoplastic corn starch-based film/paper composites have good application properties as a potential source of bioplastic materials.
33990962	7	33	theme	MCC	1181:1183	arg1	composites					1210:1219	MCC reinforced paper-plastic composites	1181:1219	MCC reinforced paper-plastic composites	1181:1219	Furthermore, MCC reinforced paper-plastic composites demonstrated good barrier properties which can meet the requirement of the need for lower water sensitive materials in the food packaging industry.
33990962	5	34	theme	folding	984:990	arg1	resistance					992:1001	folding resistance	984:1001	folding resistance	984:1001	TPS/paper composites and MCC@TPS/paper composites have better physical properties (i.e. smoothness, flexibility and folding resistance) than only paper.
33990962	6	35	theme	water	1058:1062	arg1	higher					1136:1141	higher	1136:1141	higher	1136:1141	In particular, it was found that the water contact angle of MCC@TPS/paper composites and TPS/paper composites were higher than single-layer paper.
33990962	6	35	theme	water	1058:1062	arg1	angle					1072:1076	the water contact angle	1054:1076	the water contact angle of MCC@TPS/paper composites and TPS/paper composites	1054:1129	In particular, it was found that the water contact angle of MCC@TPS/paper composites and TPS/paper composites were higher than single-layer paper.
33990962	2	36	theme	microscopy	545:554	arg1	images					556:561	the scannign elctron microscopy images	524:561	the scannign elctron microscopy images	524:561	RESULTS The paper-plastic composites exhibited good interfacial adhesion from the scannign elctron microscopy images.
33990962	4	37	theme	MCC	842:844	arg1	composites					856:865	MCC@TPS/paper composites	842:865	MCC@TPS/paper composites	842:865	% MCC was added to reinforce the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites.
33990962	2	38	theme	paper-plastic	458:470	arg1	composites					472:481	The paper-plastic composites	454:481	The paper-plastic composites	454:481	RESULTS The paper-plastic composites exhibited good interfacial adhesion from the scannign elctron microscopy images.
33990962	0	39	theme	thermoplastic	35:47	arg1	composites					78:87	thermoplastic corn starch-based film/paper composites	35:87	thermoplastic corn starch-based film/paper composites	35:87	Processing and characterization of thermoplastic corn starch-based film/paper composites containing microcrystalline cellulose.
33990962	2	40	theme	scannign	528:535	arg1	images					556:561	the scannign elctron microscopy images	524:561	the scannign elctron microscopy images	524:561	RESULTS The paper-plastic composites exhibited good interfacial adhesion from the scannign elctron microscopy images.
33990962	4	41	theme	%	586:586	arg1	MCC					588:590	% MCC	586:590	% MCC	586:590	% MCC was added to reinforce the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites.
33990962	4	42	theme	TPS/paper	846:854	arg1	composites					856:865	MCC@TPS/paper composites	842:865	MCC@TPS/paper composites	842:865	% MCC was added to reinforce the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites.
33990962	0	43	theme	starch-based	54:65	arg1	composites					78:87	thermoplastic corn starch-based film/paper composites	35:87	thermoplastic corn starch-based film/paper composites	35:87	Processing and characterization of thermoplastic corn starch-based film/paper composites containing microcrystalline cellulose.
33990962	7	44	theme	water	1311:1315	arg1	materials					1327:1335	lower water sensitive materials	1305:1335	lower water sensitive materials	1305:1335	Furthermore, MCC reinforced paper-plastic composites demonstrated good barrier properties which can meet the requirement of the need for lower water sensitive materials in the food packaging industry.
33990962	4	45	theme	vapor	815:819	arg1	rate					834:837	the water vapor transmission rate	805:837	the water vapor transmission rate of MCC@TPS/paper composites	805:865	% MCC was added to reinforce the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites.
33990962	6	46	theme	composites	1120:1129	arg1	higher					1136:1141	higher	1136:1141	higher	1136:1141	In particular, it was found that the water contact angle of MCC@TPS/paper composites and TPS/paper composites were higher than single-layer paper.
33990962	6	46	theme	composites	1120:1129	arg1	angle					1072:1076	the water contact angle	1054:1076	the water contact angle of MCC@TPS/paper composites and TPS/paper composites	1054:1129	In particular, it was found that the water contact angle of MCC@TPS/paper composites and TPS/paper composites were higher than single-layer paper.
33990962	8	47	theme	bioplastic	1492:1501	arg1	materials					1503:1511	bioplastic materials	1492:1511	bioplastic materials	1492:1511	CONCLUSION Thermoplastic corn starch-based film/paper composites have good application properties as a potential source of bioplastic materials.
33990962	6	48	theme	TPS/paper	1085:1093	arg1	composites					1095:1104	MCC@TPS/paper composites	1081:1104	MCC@TPS/paper composites	1081:1104	In particular, it was found that the water contact angle of MCC@TPS/paper composites and TPS/paper composites were higher than single-layer paper.
33990962	4	49	theme	TPS	644:646	arg1	films					648:652	TPS films	644:652	TPS films	644:652	% MCC was added to reinforce the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites.
33990962	4	50	theme	composites	856:865	arg1	rate					834:837	the water vapor transmission rate	805:837	the water vapor transmission rate of MCC@TPS/paper composites	805:865	% MCC was added to reinforce the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites.
33990962	5	51	theme	@	896:896	arg1	composites					907:916	MCC@TPS/paper composites	893:916	MCC@TPS/paper composites	893:916	TPS/paper composites and MCC@TPS/paper composites have better physical properties (i.e. smoothness, flexibility and folding resistance) than only paper.
33990962	4	52	theme	MCC	708:710	arg1	composites					722:731	MCC@TPS/paper composites	708:731	MCC@TPS/paper composites	708:731	% MCC was added to reinforce the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites.
33990962	4	53	theme	vapor	764:768	arg1	path					750:753	the path	746:753	the path of water vapor	746:768	% MCC was added to reinforce the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites.
33990962	6	54	theme	single-layer	1148:1159	arg1	paper					1161:1165	single-layer paper	1148:1165	single-layer paper	1148:1165	In particular, it was found that the water contact angle of MCC@TPS/paper composites and TPS/paper composites were higher than single-layer paper.
33990962	5	55	theme	TPS/paper	868:876	arg1	composites					878:887	TPS/paper composites	868:887	TPS/paper composites	868:887	TPS/paper composites and MCC@TPS/paper composites have better physical properties (i.e. smoothness, flexibility and folding resistance) than only paper.
33990962	1	56	theme	starch	163:168	arg1	films					176:180	BACKGROUND Different thermoplastic starch (TPS) films	128:180	BACKGROUND Different thermoplastic starch (TPS) films	128:180	BACKGROUND Different thermoplastic starch (TPS) films were prepared with or without the addition of microcrystalline cellulose (MCC) obtained via the melt-extrusion method, and then the hot-press method was used to produce environmentally friendly TPS-based film/paper composites to replace petroleum-based materials.
33990962	5	57	contain	have	918:921	arg1	composites					907:916	MCC@TPS/paper composites	893:916	MCC@TPS/paper composites	893:916	TPS/paper composites and MCC@TPS/paper composites have better physical properties (i.e. smoothness, flexibility and folding resistance) than only paper.
33990962	5	57	contain	have	918:921	arg1	composites					878:887	TPS/paper composites	868:887	TPS/paper composites	868:887	TPS/paper composites and MCC@TPS/paper composites have better physical properties (i.e. smoothness, flexibility and folding resistance) than only paper.
33990962	5	57	contain	have	918:921	arg2	properties					939:948	better physical properties	923:948	better physical properties (i.e. smoothness, flexibility and folding resistance)	923:1002	TPS/paper composites and MCC@TPS/paper composites have better physical properties (i.e. smoothness, flexibility and folding resistance) than only paper.
33990962	4	58	theme	TPS/paper	712:720	arg1	composites					722:731	MCC@TPS/paper composites	708:731	MCC@TPS/paper composites	708:731	% MCC was added to reinforce the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites.
33990962	1	59	theme	environmentally	351:365	arg1	composites					397:406	environmentally friendly TPS-based film/paper composites	351:406	environmentally friendly TPS-based film/paper composites	351:406	BACKGROUND Different thermoplastic starch (TPS) films were prepared with or without the addition of microcrystalline cellulose (MCC) obtained via the melt-extrusion method, and then the hot-press method was used to produce environmentally friendly TPS-based film/paper composites to replace petroleum-based materials.
33990962	5	60	theme	better	923:928	arg1	properties					939:948	better physical properties	923:948	better physical properties (i.e. smoothness, flexibility and folding resistance)	923:1002	TPS/paper composites and MCC@TPS/paper composites have better physical properties (i.e. smoothness, flexibility and folding resistance) than only paper.
33990962	7	61	theme	good	1234:1237	arg1	properties					1247:1256	good barrier properties	1234:1256	good barrier properties which can meet the requirement of the need for lower water sensitive materials in the food packaging industry	1234:1366	Furthermore, MCC reinforced paper-plastic composites demonstrated good barrier properties which can meet the requirement of the need for lower water sensitive materials in the food packaging industry.
33990962	1	62	theme	TPS-based	376:384	arg1	composites					397:406	environmentally friendly TPS-based film/paper composites	351:406	environmentally friendly TPS-based film/paper composites	351:406	BACKGROUND Different thermoplastic starch (TPS) films were prepared with or without the addition of microcrystalline cellulose (MCC) obtained via the melt-extrusion method, and then the hot-press method was used to produce environmentally friendly TPS-based film/paper composites to replace petroleum-based materials.
33990962	5	63	dep	smoothness	956:965	arg1	i.e.					951:954	i.e.	951:954	i.e.	951:954	TPS/paper composites and MCC@TPS/paper composites have better physical properties (i.e. smoothness, flexibility and folding resistance) than only paper.
33990962	8	64	theme	CONCLUSION	1369:1378	arg1	composites					1423:1432	CONCLUSION Thermoplastic corn starch-based film/paper composites	1369:1432	CONCLUSION Thermoplastic corn starch-based film/paper composites	1369:1432	CONCLUSION Thermoplastic corn starch-based film/paper composites have good application properties as a potential source of bioplastic materials.
33990962	6	65	theme	@	1084:1084	arg1	composites					1095:1104	MCC@TPS/paper composites	1081:1104	MCC@TPS/paper composites	1081:1104	In particular, it was found that the water contact angle of MCC@TPS/paper composites and TPS/paper composites were higher than single-layer paper.
33990962	8	66	theme	corn	1394:1397	arg1	composites					1423:1432	CONCLUSION Thermoplastic corn starch-based film/paper composites	1369:1432	CONCLUSION Thermoplastic corn starch-based film/paper composites	1369:1432	CONCLUSION Thermoplastic corn starch-based film/paper composites have good application properties as a potential source of bioplastic materials.
33990962	7	67	theme	reinforced	1185:1194	arg1	composites					1210:1219	MCC reinforced paper-plastic composites	1181:1219	MCC reinforced paper-plastic composites	1181:1219	Furthermore, MCC reinforced paper-plastic composites demonstrated good barrier properties which can meet the requirement of the need for lower water sensitive materials in the food packaging industry.
33990962	7	68	theme	food	1344:1347	arg1	industry					1359:1366	the food packaging industry	1340:1366	the food packaging industry	1340:1366	Furthermore, MCC reinforced paper-plastic composites demonstrated good barrier properties which can meet the requirement of the need for lower water sensitive materials in the food packaging industry.
33990962	6	69	located	found	1043:1047	arg2	it					1036:1037	it	1036:1037	it	1036:1037	In particular, it was found that the water contact angle of MCC@TPS/paper composites and TPS/paper composites were higher than single-layer paper.
33990962	6	69	located	found	1043:1047	arg1	particular					1024:1033	particular	1024:1033	particular	1024:1033	In particular, it was found that the water contact angle of MCC@TPS/paper composites and TPS/paper composites were higher than single-layer paper.
33990962	2	70	dep	RESULTS	446:452	arg1	exhibited					483:491	exhibited	483:491	exhibited good interfacial adhesion from the scannign elctron microscopy images	483:561	RESULTS The paper-plastic composites exhibited good interfacial adhesion from the scannign elctron microscopy images.
33990962	1	71	theme	Different	139:147	arg1	TPS					171:173	TPS	171:173	TPS	171:173	BACKGROUND Different thermoplastic starch (TPS) films were prepared with or without the addition of microcrystalline cellulose (MCC) obtained via the melt-extrusion method, and then the hot-press method was used to produce environmentally friendly TPS-based film/paper composites to replace petroleum-based materials.
33990962	1	71	theme	Different	139:147	arg1	starch					163:168	BACKGROUND Different thermoplastic starch	128:168	BACKGROUND Different thermoplastic starch (TPS) films	128:180	BACKGROUND Different thermoplastic starch (TPS) films were prepared with or without the addition of microcrystalline cellulose (MCC) obtained via the melt-extrusion method, and then the hot-press method was used to produce environmentally friendly TPS-based film/paper composites to replace petroleum-based materials.
33990962	6	72	theme	contact	1064:1070	arg1	higher					1136:1141	higher	1136:1141	higher	1136:1141	In particular, it was found that the water contact angle of MCC@TPS/paper composites and TPS/paper composites were higher than single-layer paper.
33990962	6	72	theme	contact	1064:1070	arg1	angle					1072:1076	the water contact angle	1054:1076	the water contact angle of MCC@TPS/paper composites and TPS/paper composites	1054:1129	In particular, it was found that the water contact angle of MCC@TPS/paper composites and TPS/paper composites were higher than single-layer paper.
33990962	2	73	theme	good	493:496	arg1	adhesion					510:517	good interfacial adhesion	493:517	good interfacial adhesion	493:517	RESULTS The paper-plastic composites exhibited good interfacial adhesion from the scannign elctron microscopy images.
33990962	4	74	theme	mechanical	619:628	arg1	properties					630:639	the mechanical properties	615:639	the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites	615:865	% MCC was added to reinforce the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites.
33990962	4	75	theme	TPS	778:780	arg1	films					782:786	TPS films	778:786	TPS films	778:786	% MCC was added to reinforce the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites.
33990962	0	76	theme	corn	49:52	arg1	composites					78:87	thermoplastic corn starch-based film/paper composites	35:87	thermoplastic corn starch-based film/paper composites	35:87	Processing and characterization of thermoplastic corn starch-based film/paper composites containing microcrystalline cellulose.
33990962	1	77	theme	hot-press	314:322	arg1	method					324:329	then the hot-press method	305:329	then the hot-press method	305:329	BACKGROUND Different thermoplastic starch (TPS) films were prepared with or without the addition of microcrystalline cellulose (MCC) obtained via the melt-extrusion method, and then the hot-press method was used to produce environmentally friendly TPS-based film/paper composites to replace petroleum-based materials.
33990962	1	78	theme	cellulose	245:253	arg1	addition					216:223	the addition	212:223	the addition of microcrystalline cellulose (MCC) obtained via the melt-extrusion method	212:298	BACKGROUND Different thermoplastic starch (TPS) films were prepared with or without the addition of microcrystalline cellulose (MCC) obtained via the melt-extrusion method, and then the hot-press method was used to produce environmentally friendly TPS-based film/paper composites to replace petroleum-based materials.
33990962	7	79	theme	sensitive	1317:1325	arg1	materials					1327:1335	lower water sensitive materials	1305:1335	lower water sensitive materials	1305:1335	Furthermore, MCC reinforced paper-plastic composites demonstrated good barrier properties which can meet the requirement of the need for lower water sensitive materials in the food packaging industry.
33990962	2	80	theme	elctron	537:543	arg1	images					556:561	the scannign elctron microscopy images	524:561	the scannign elctron microscopy images	524:561	RESULTS The paper-plastic composites exhibited good interfacial adhesion from the scannign elctron microscopy images.
33990962	8	81	theme	application	1444:1454	arg1	source					1482:1487	a potential source	1470:1487	a potential source of bioplastic materials	1470:1511	CONCLUSION Thermoplastic corn starch-based film/paper composites have good application properties as a potential source of bioplastic materials.
33990962	8	81	theme	application	1444:1454	arg1	properties					1456:1465	good application properties	1439:1465	good application properties	1439:1465	CONCLUSION Thermoplastic corn starch-based film/paper composites have good application properties as a potential source of bioplastic materials.
33990962	0	82	theme	film/paper	67:76	arg1	composites					78:87	thermoplastic corn starch-based film/paper composites	35:87	thermoplastic corn starch-based film/paper composites	35:87	Processing and characterization of thermoplastic corn starch-based film/paper composites containing microcrystalline cellulose.
33990962	9	83	theme	Chemical	1532:1539	arg1	Industry					1541:1548	Chemical Industry	1532:1548	Chemical Industry	1532:1548	© 2021 Society of Chemical Industry.
33990962	7	84	theme	lower	1305:1309	arg1	materials					1327:1335	lower water sensitive materials	1305:1335	lower water sensitive materials	1305:1335	Furthermore, MCC reinforced paper-plastic composites demonstrated good barrier properties which can meet the requirement of the need for lower water sensitive materials in the food packaging industry.
33990962	4	85	theme	@	845:845	arg1	composites					856:865	MCC@TPS/paper composites	842:865	MCC@TPS/paper composites	842:865	% MCC was added to reinforce the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites.
33990962	7	86	theme	need	1296:1299	arg1	requirement					1277:1287	the requirement	1273:1287	the requirement of the need for lower water sensitive materials in the food packaging industry	1273:1366	Furthermore, MCC reinforced paper-plastic composites demonstrated good barrier properties which can meet the requirement of the need for lower water sensitive materials in the food packaging industry.
33990962	8	87	theme	potential	1472:1480	arg1	source					1482:1487	a potential source	1470:1487	a potential source of bioplastic materials	1470:1511	CONCLUSION Thermoplastic corn starch-based film/paper composites have good application properties as a potential source of bioplastic materials.
33990962	8	87	theme	potential	1472:1480	arg1	properties					1456:1465	good application properties	1439:1465	good application properties	1439:1465	CONCLUSION Thermoplastic corn starch-based film/paper composites have good application properties as a potential source of bioplastic materials.
33990962	6	88	theme	TPS/paper	1110:1118	arg1	composites					1120:1129	TPS/paper composites	1110:1129	TPS/paper composites	1110:1129	In particular, it was found that the water contact angle of MCC@TPS/paper composites and TPS/paper composites were higher than single-layer paper.
33990962	4	89	theme	transmission	821:832	arg1	rate					834:837	the water vapor transmission rate	805:837	the water vapor transmission rate of MCC@TPS/paper composites	805:865	% MCC was added to reinforce the mechanical properties of TPS films, such that it also improved the barrier properties of MCC@TPS/paper composites and extended the path of water vapor through TPS films, which decreased the water vapor transmission rate of MCC@TPS/paper composites.
32580493	0	0	theme	Phenolic	97:104	arg1	Compounds					106:114	Phenolic Compounds	97:114	Phenolic Compounds	97:114	Laccase-Based Biosensor Encapsulated in a Galactomannan-Chitosan Composite for the Evaluation of Phenolic Compounds.
32580493	2	1	used	used	215:218	arg2	It					208:209	It	208:209	It	208:209	It was used for the first time for the fabrication of a laccase-based biosensor by the encapsulation of laccase in a chitosan+galactomannan composite.
32580493	5	2	theme	detection	691:699	arg1	sensitivity					676:686	the sensitivity	672:686	the sensitivity of detection of pyrocatechol in the 10-16 M-10-4 M range	672:743	The laccase activity was well preserved in the chitosan+galactomannan composite and the sensitivity of detection of pyrocatechol in the 10-16 M-10-4 M range was very high.
32580493	5	2	theme	detection	691:699	arg1	high					754:757	high	754:757	high	754:757	The laccase activity was well preserved in the chitosan+galactomannan composite and the sensitivity of detection of pyrocatechol in the 10-16 M-10-4 M range was very high.
32580493	2	3	from	encapsulation	295:307	arg1	composite					348:356	a chitosan+galactomannan composite	323:356	a chitosan+galactomannan composite	323:356	It was used for the first time for the fabrication of a laccase-based biosensor by the encapsulation of laccase in a chitosan+galactomannan composite.
32580493	1	4	theme	carob	177:181	arg1	seeds					183:187	carob seeds	177:187	carob seeds	177:187	Galactomannan, a neutral polysaccharide, was extracted from carob seeds and characterized.
32580493	5	5	theme	pyrocatechol	704:715	arg1	detection					691:699	detection	691:699	detection of pyrocatechol in the 10-16 M-10-4 M range	691:743	The laccase activity was well preserved in the chitosan+galactomannan composite and the sensitivity of detection of pyrocatechol in the 10-16 M-10-4 M range was very high.
32580493	7	6	theme	colorimetric	986:997	arg1	method					1015:1020	the colorimetric Folin-Ciocalteu method	982:1020	the colorimetric Folin-Ciocalteu method	982:1020	To estimate the antioxidant capacity of olive oil samples, it was shown that the obtained laccase-based biosensor is a valuable alternative to the colorimetric Folin-Ciocalteu method.
32580493	5	7	from	range	739:743	arg1	sensitivity					676:686	the sensitivity	672:686	the sensitivity of detection of pyrocatechol in the 10-16 M-10-4 M range	672:743	The laccase activity was well preserved in the chitosan+galactomannan composite and the sensitivity of detection of pyrocatechol in the 10-16 M-10-4 M range was very high.
32580493	5	7	from	range	739:743	arg1	high					754:757	high	754:757	high	754:757	The laccase activity was well preserved in the chitosan+galactomannan composite and the sensitivity of detection of pyrocatechol in the 10-16 M-10-4 M range was very high.
32580493	0	8	theme	Compounds	106:114	arg1	Evaluation					83:92	the Evaluation	79:92	the Evaluation of Phenolic Compounds	79:114	Laccase-Based Biosensor Encapsulated in a Galactomannan-Chitosan Composite for the Evaluation of Phenolic Compounds.
32580493	4	9	theme	cyclic	507:512	arg1	measurements					526:537	cyclic voltammetry measurements	507:537	cyclic voltammetry measurements	507:537	The pyrocatechol detection was obtained by cyclic voltammetry measurements, through the detection of o-quinone at -0.447 V.
32580493	5	10	theme	chitosan+galactomannan	635:656	arg1	composite					658:666	the chitosan+galactomannan composite	631:666	the chitosan+galactomannan composite	631:666	The laccase activity was well preserved in the chitosan+galactomannan composite and the sensitivity of detection of pyrocatechol in the 10-16 M-10-4 M range was very high.
32580493	7	11	theme	oil	885:887	arg1	samples					889:895	olive oil samples	879:895	olive oil samples	879:895	To estimate the antioxidant capacity of olive oil samples, it was shown that the obtained laccase-based biosensor is a valuable alternative to the colorimetric Folin-Ciocalteu method.
32580493	2	12	theme	biosensor	278:286	arg1	fabrication					247:257	the fabrication	243:257	the fabrication of a laccase-based biosensor by the encapsulation of laccase in a chitosan+galactomannan composite	243:356	It was used for the first time for the fabrication of a laccase-based biosensor by the encapsulation of laccase in a chitosan+galactomannan composite.
32580493	7	13	theme	samples	889:895	arg1	capacity					867:874	the antioxidant capacity	851:874	the antioxidant capacity of olive oil samples	851:895	To estimate the antioxidant capacity of olive oil samples, it was shown that the obtained laccase-based biosensor is a valuable alternative to the colorimetric Folin-Ciocalteu method.
32580493	3	14	theme	fabricated	363:372	arg1	biosensor					374:382	The fabricated biosensor	359:382	The fabricated biosensor	359:382	The fabricated biosensor was characterized by FTIR, scanning electron microscopy and cyclic voltammetry.
32580493	2	15	theme	laccase-based	264:276	arg1	biosensor					278:286	a laccase-based biosensor	262:286	a laccase-based biosensor by the encapsulation of laccase in a chitosan+galactomannan composite	262:356	It was used for the first time for the fabrication of a laccase-based biosensor by the encapsulation of laccase in a chitosan+galactomannan composite.
32580493	4	16	theme	voltammetry	514:524	arg1	measurements					526:537	cyclic voltammetry measurements	507:537	cyclic voltammetry measurements	507:537	The pyrocatechol detection was obtained by cyclic voltammetry measurements, through the detection of o-quinone at -0.447 V.
32580493	7	17	theme	valuable	958:965	arg1	biosensor					943:951	the obtained laccase-based biosensor	916:951	the obtained laccase-based biosensor	916:951	To estimate the antioxidant capacity of olive oil samples, it was shown that the obtained laccase-based biosensor is a valuable alternative to the colorimetric Folin-Ciocalteu method.
32580493	7	17	theme	valuable	958:965	arg1	alternative					967:977	a valuable alternative	956:977	a valuable alternative to the colorimetric Folin-Ciocalteu method	956:1020	To estimate the antioxidant capacity of olive oil samples, it was shown that the obtained laccase-based biosensor is a valuable alternative to the colorimetric Folin-Ciocalteu method.
32580493	7	18	theme	olive	879:883	arg1	samples					889:895	olive oil samples	879:895	olive oil samples	879:895	To estimate the antioxidant capacity of olive oil samples, it was shown that the obtained laccase-based biosensor is a valuable alternative to the colorimetric Folin-Ciocalteu method.
32580493	3	19	theme	cyclic	444:449	arg1	voltammetry					451:461	cyclic voltammetry	444:461	cyclic voltammetry	444:461	The fabricated biosensor was characterized by FTIR, scanning electron microscopy and cyclic voltammetry.
32580493	0	20	theme	Laccase-Based	0:12	arg1	Biosensor					14:22	Laccase-Based Biosensor	0:22	Laccase-Based Biosensor	0:22	Laccase-Based Biosensor Encapsulated in a Galactomannan-Chitosan Composite for the Evaluation of Phenolic Compounds.
32580493	5	21	from	sensitivity	676:686	arg1	range					739:743	the 10-16 M-10-4 M range	720:743	the 10-16 M-10-4 M range	720:743	The laccase activity was well preserved in the chitosan+galactomannan composite and the sensitivity of detection of pyrocatechol in the 10-16 M-10-4 M range was very high.
32580493	2	22	theme	laccase	312:318	arg1	encapsulation					295:307	the encapsulation	291:307	the encapsulation of laccase in a chitosan+galactomannan composite	291:356	It was used for the first time for the fabrication of a laccase-based biosensor by the encapsulation of laccase in a chitosan+galactomannan composite.
32580493	7	23	theme	antioxidant	855:865	arg1	capacity					867:874	the antioxidant capacity	851:874	the antioxidant capacity of olive oil samples	851:895	To estimate the antioxidant capacity of olive oil samples, it was shown that the obtained laccase-based biosensor is a valuable alternative to the colorimetric Folin-Ciocalteu method.
32580493	4	24	from	V	585:585	arg1	detection					552:560	the detection	548:560	the detection of o-quinone at -0.447 V	548:585	The pyrocatechol detection was obtained by cyclic voltammetry measurements, through the detection of o-quinone at -0.447 V.
32580493	0	25	theme	Galactomannan-Chitosan	42:63	arg1	Composite					65:73	a Galactomannan-Chitosan Composite	40:73	a Galactomannan-Chitosan Composite for the Evaluation of Phenolic Compounds	40:114	Laccase-Based Biosensor Encapsulated in a Galactomannan-Chitosan Composite for the Evaluation of Phenolic Compounds.
32580493	4	26	theme	pyrocatechol	468:479	arg1	detection					481:489	The pyrocatechol detection	464:489	The pyrocatechol detection	464:489	The pyrocatechol detection was obtained by cyclic voltammetry measurements, through the detection of o-quinone at -0.447 V.
32580493	4	27	theme	o-quinone	565:573	arg1	detection					552:560	the detection	548:560	the detection of o-quinone at -0.447 V	548:585	The pyrocatechol detection was obtained by cyclic voltammetry measurements, through the detection of o-quinone at -0.447 V.
32580493	3	28	theme	scanning	411:418	arg1	microscopy					429:438	scanning electron microscopy	411:438	scanning electron microscopy	411:438	The fabricated biosensor was characterized by FTIR, scanning electron microscopy and cyclic voltammetry.
32580493	2	29	theme	first	228:232	arg1	time					234:237	the first time	224:237	the first time for the fabrication of a laccase-based biosensor by the encapsulation of laccase in a chitosan+galactomannan composite	224:356	It was used for the first time for the fabrication of a laccase-based biosensor by the encapsulation of laccase in a chitosan+galactomannan composite.
32580493	6	30	theme	biosensor	793:801	arg1	stable					807:812	stable	807:812	stable	807:812	The voltammetric response of the biosensor was stable for more than two weeks.
32580493	6	30	theme	biosensor	793:801	arg1	response					777:784	The voltammetric response	760:784	The voltammetric response of the biosensor	760:801	The voltammetric response of the biosensor was stable for more than two weeks.
32580493	3	31	theme	electron	420:427	arg1	microscopy					429:438	scanning electron microscopy	411:438	scanning electron microscopy	411:438	The fabricated biosensor was characterized by FTIR, scanning electron microscopy and cyclic voltammetry.
32580493	5	32	from	detection	691:699	arg1	range					739:743	the 10-16 M-10-4 M range	720:743	the 10-16 M-10-4 M range	720:743	The laccase activity was well preserved in the chitosan+galactomannan composite and the sensitivity of detection of pyrocatechol in the 10-16 M-10-4 M range was very high.
32580493	5	33	theme	10-16	724:728	arg1	M-10-4					730:735	M-10-4	730:735	M-10-4	730:735	The laccase activity was well preserved in the chitosan+galactomannan composite and the sensitivity of detection of pyrocatechol in the 10-16 M-10-4 M range was very high.
32580493	1	34	theme	neutral	134:140	arg1	polysaccharide					142:155	a neutral polysaccharide	132:155	a neutral polysaccharide	132:155	Galactomannan, a neutral polysaccharide, was extracted from carob seeds and characterized.
32580493	1	34	theme	neutral	134:140	arg1	Galactomannan					117:129	Galactomannan	117:129	Galactomannan	117:129	Galactomannan, a neutral polysaccharide, was extracted from carob seeds and characterized.
32580493	7	35	theme	obtained	920:927	arg1	biosensor					943:951	the obtained laccase-based biosensor	916:951	the obtained laccase-based biosensor	916:951	To estimate the antioxidant capacity of olive oil samples, it was shown that the obtained laccase-based biosensor is a valuable alternative to the colorimetric Folin-Ciocalteu method.
32580493	7	35	theme	obtained	920:927	arg1	alternative					967:977	a valuable alternative	956:977	a valuable alternative to the colorimetric Folin-Ciocalteu method	956:1020	To estimate the antioxidant capacity of olive oil samples, it was shown that the obtained laccase-based biosensor is a valuable alternative to the colorimetric Folin-Ciocalteu method.
32580493	5	36	theme	M-10-4	730:735	arg1	range					739:743	the 10-16 M-10-4 M range	720:743	the 10-16 M-10-4 M range	720:743	The laccase activity was well preserved in the chitosan+galactomannan composite and the sensitivity of detection of pyrocatechol in the 10-16 M-10-4 M range was very high.
32580493	5	37	theme	laccase	592:598	arg1	activity					600:607	The laccase activity	588:607	The laccase activity	588:607	The laccase activity was well preserved in the chitosan+galactomannan composite and the sensitivity of detection of pyrocatechol in the 10-16 M-10-4 M range was very high.
32580493	2	38	theme	chitosan+galactomannan	325:346	arg1	composite					348:356	a chitosan+galactomannan composite	323:356	a chitosan+galactomannan composite	323:356	It was used for the first time for the fabrication of a laccase-based biosensor by the encapsulation of laccase in a chitosan+galactomannan composite.
32580493	7	39	theme	laccase-based	929:941	arg1	biosensor					943:951	the obtained laccase-based biosensor	916:951	the obtained laccase-based biosensor	916:951	To estimate the antioxidant capacity of olive oil samples, it was shown that the obtained laccase-based biosensor is a valuable alternative to the colorimetric Folin-Ciocalteu method.
32580493	7	39	theme	laccase-based	929:941	arg1	alternative					967:977	a valuable alternative	956:977	a valuable alternative to the colorimetric Folin-Ciocalteu method	956:1020	To estimate the antioxidant capacity of olive oil samples, it was shown that the obtained laccase-based biosensor is a valuable alternative to the colorimetric Folin-Ciocalteu method.
32580493	5	40	theme	M	737:737	arg1	range					739:743	the 10-16 M-10-4 M range	720:743	the 10-16 M-10-4 M range	720:743	The laccase activity was well preserved in the chitosan+galactomannan composite and the sensitivity of detection of pyrocatechol in the 10-16 M-10-4 M range was very high.
32580493	6	41	theme	voltammetric	764:775	arg1	stable					807:812	stable	807:812	stable	807:812	The voltammetric response of the biosensor was stable for more than two weeks.
32580493	6	41	theme	voltammetric	764:775	arg1	response					777:784	The voltammetric response	760:784	The voltammetric response of the biosensor	760:801	The voltammetric response of the biosensor was stable for more than two weeks.
32580493	7	42	theme	Folin-Ciocalteu	999:1013	arg1	method					1015:1020	the colorimetric Folin-Ciocalteu method	982:1020	the colorimetric Folin-Ciocalteu method	982:1020	To estimate the antioxidant capacity of olive oil samples, it was shown that the obtained laccase-based biosensor is a valuable alternative to the colorimetric Folin-Ciocalteu method.
34478790	5	0	theme	significant	772:782	arg1	changes					784:790	No significant changes	769:790	No significant changes	769:790	No significant changes were observed in the types and ratios of glycosidic linkages, as determined by NMR spectroscopy.
34478790	2	1	attach	isolated	372:379	arg2	MPN					367:369	MPN	367:369	MPN	367:369	The purpose of this research is to determine the influence of selenium on the structure and bioactivity of a polysaccharide fraction (MPN) isolated from Ganoderma lucidum mycelia.
34478790	2	1	attach	isolated	372:379	arg1	mycelia					404:410	Ganoderma lucidum mycelia	386:410	Ganoderma lucidum mycelia	386:410	The purpose of this research is to determine the influence of selenium on the structure and bioactivity of a polysaccharide fraction (MPN) isolated from Ganoderma lucidum mycelia.
34478790	2	1	attach	isolated	372:379	arg2	fraction					357:364	a polysaccharide fraction	340:364	a polysaccharide fraction (MPN) isolated from Ganoderma lucidum mycelia	340:410	The purpose of this research is to determine the influence of selenium on the structure and bioactivity of a polysaccharide fraction (MPN) isolated from Ganoderma lucidum mycelia.
34478790	0	2	theme	polysaccharide	75:88	arg1	bioactivities					38:50	bioactivities	38:50	bioactivities of a natural selenized polysaccharide from Ganoderma Lucidum mycelia	38:119	Purification and comparative study of bioactivities of a natural selenized polysaccharide from Ganoderma Lucidum mycelia.
34478790	1	3	theme	promising	172:180	arg1	strategy					182:189	a promising strategy	170:189	a promising strategy for the dietary selenium supplementation	170:230	The development of selenized polysaccharides is a promising strategy for the dietary selenium supplementation.
34478790	1	3	theme	promising	172:180	arg1	development					126:136	The development	122:136	The development of selenized polysaccharides	122:165	The development of selenized polysaccharides is a promising strategy for the dietary selenium supplementation.
34478790	8	4	theme	functional	1356:1365	arg1	supplementation					1376:1390	potential functional selenium supplementation	1346:1390	potential functional selenium supplementation	1346:1390	SeMPN could be as potential functional selenium supplementation.
34478790	0	5	theme	Lucidum	105:111	arg1	mycelia					113:119	Ganoderma Lucidum mycelia	95:119	Ganoderma Lucidum mycelia	95:119	Purification and comparative study of bioactivities of a natural selenized polysaccharide from Ganoderma Lucidum mycelia.
34478790	6	6	theme	antioxidant	921:931	arg1	assays					933:938	Extracellular and intracellular antioxidant assays	889:938	Extracellular and intracellular antioxidant assays	889:938	Extracellular and intracellular antioxidant assays demonstrated that SeMPN was more effective than MPN in scavenging free radicals, inhibiting AAPH-induced erythrocyte hemolysis, and protecting catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells.
34478790	5	7	dep	types	813:817	arg1	the					809:811	the	809:811	the	809:811	No significant changes were observed in the types and ratios of glycosidic linkages, as determined by NMR spectroscopy.
34478790	7	8	theme	NO	1307:1308	arg1	capacity					1266:1273	pinocytic and phagocytic capacity	1241:1273	pinocytic and phagocytic capacity	1241:1273	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	7	8	theme	NO	1307:1308	arg1	cells					1233:1237	RAW 264.7 cells	1223:1237	RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6	1223:1325	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	7	8	theme	NO	1307:1308	arg1	production					1293:1302	their production	1287:1302	their production of NO	1287:1308	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	7	8	theme	NO	1307:1308	arg1	TNF-α					1311:1315	TNF-α	1311:1315	TNF-α	1311:1315	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	7	8	theme	NO	1307:1308	arg1	IL-6					1322:1325	IL-6	1322:1325	IL-6	1322:1325	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	3	9	theme	lower	555:559	arg1	weight					571:576	a slightly lower molecular weight	544:576	a slightly lower molecular weight than MPN	544:585	After biological selenium enrichment, the selenium content in the selenized polysaccharide (SeMPN) was 18.91 ± 1.8 μg/g. SeMPN had a slightly lower molecular weight than MPN, but the carbohydrate content and monosaccharide composition remained identical.
34478790	3	10	dep	had	540:542	arg1	SeMPN					534:538	18.91 ± 1.8 μg/g. SeMPN	516:538	18.91 ± 1.8 μg/g. SeMPN	516:538	After biological selenium enrichment, the selenium content in the selenized polysaccharide (SeMPN) was 18.91 ± 1.8 μg/g. SeMPN had a slightly lower molecular weight than MPN, but the carbohydrate content and monosaccharide composition remained identical.
34478790	0	11	theme	Ganoderma	95:103	arg1	Lucidum					105:111	Ganoderma Lucidum	95:111	Ganoderma Lucidum mycelia	95:119	Purification and comparative study of bioactivities of a natural selenized polysaccharide from Ganoderma Lucidum mycelia.
34478790	6	12	theme	intracellular	907:919	arg1	assays					933:938	Extracellular and intracellular antioxidant assays	889:938	Extracellular and intracellular antioxidant assays	889:938	Extracellular and intracellular antioxidant assays demonstrated that SeMPN was more effective than MPN in scavenging free radicals, inhibiting AAPH-induced erythrocyte hemolysis, and protecting catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells.
34478790	3	13	theme	molecular	561:569	arg1	weight					571:576	a slightly lower molecular weight	544:576	a slightly lower molecular weight than MPN	544:585	After biological selenium enrichment, the selenium content in the selenized polysaccharide (SeMPN) was 18.91 ± 1.8 μg/g. SeMPN had a slightly lower molecular weight than MPN, but the carbohydrate content and monosaccharide composition remained identical.
34478790	7	14	theme	phagocytic	1255:1264	arg1	capacity					1266:1273	pinocytic and phagocytic capacity	1241:1273	pinocytic and phagocytic capacity	1241:1273	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	7	14	theme	phagocytic	1255:1264	arg1	cells					1233:1237	RAW 264.7 cells	1223:1237	RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6	1223:1325	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	2	15	theme	selenium	295:302	arg1	influence					282:290	the influence	278:290	the influence of selenium on the structure and bioactivity of a polysaccharide fraction (MPN) isolated from Ganoderma lucidum mycelia	278:410	The purpose of this research is to determine the influence of selenium on the structure and bioactivity of a polysaccharide fraction (MPN) isolated from Ganoderma lucidum mycelia.
34478790	4	16	from	606 cm-1	694:701	arg1	band					686:689	the band	682:689	the band at 606 cm-1 in MPN	682:708	Additionally, the band at 606 cm-1 in MPN changed to 615 cm-1 in SeMPN as revealed by FT-IR spectra.
34478790	6	17	theme	free	1006:1009	arg1	radicals					1011:1018	scavenging free radicals	995:1018	scavenging free radicals	995:1018	Extracellular and intracellular antioxidant assays demonstrated that SeMPN was more effective than MPN in scavenging free radicals, inhibiting AAPH-induced erythrocyte hemolysis, and protecting catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells.
34478790	7	18	theme	increase	1204:1211	arg1	effect					1213:1218	a higher increase effect	1195:1218	a higher increase effect	1195:1218	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	2	19	dep	structure	311:319	arg1	the					307:309	the	307:309	the	307:309	The purpose of this research is to determine the influence of selenium on the structure and bioactivity of a polysaccharide fraction (MPN) isolated from Ganoderma lucidum mycelia.
34478790	6	20	from	radicals	1011:1018	arg1	effective					973:981	effective	973:981	effective	973:981	Extracellular and intracellular antioxidant assays demonstrated that SeMPN was more effective than MPN in scavenging free radicals, inhibiting AAPH-induced erythrocyte hemolysis, and protecting catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells.
34478790	5	21	theme	linkages	844:851	arg1	ratios					823:828	ratios	823:828	ratios	823:828	No significant changes were observed in the types and ratios of glycosidic linkages, as determined by NMR spectroscopy.
34478790	5	21	theme	linkages	844:851	arg1	types					813:817	types	813:817	types	813:817	No significant changes were observed in the types and ratios of glycosidic linkages, as determined by NMR spectroscopy.
34478790	5	22	theme	NMR	871:873	arg1	spectroscopy					875:886	NMR spectroscopy	871:886	NMR spectroscopy	871:886	No significant changes were observed in the types and ratios of glycosidic linkages, as determined by NMR spectroscopy.
34478790	6	23	theme	catalase	1083:1090	arg1	activity					1134:1141	catalase (CAT) and glutathione peroxidase (GSH-Px) activity	1083:1141	catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells	1083:1168	Extracellular and intracellular antioxidant assays demonstrated that SeMPN was more effective than MPN in scavenging free radicals, inhibiting AAPH-induced erythrocyte hemolysis, and protecting catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells.
34478790	0	24	from	bioactivities	38:50	arg1	mycelia					113:119	Ganoderma Lucidum mycelia	95:119	Ganoderma Lucidum mycelia	95:119	Purification and comparative study of bioactivities of a natural selenized polysaccharide from Ganoderma Lucidum mycelia.
34478790	3	25	theme	biological	419:428	arg1	enrichment					439:448	biological selenium enrichment	419:448	biological selenium enrichment	419:448	After biological selenium enrichment, the selenium content in the selenized polysaccharide (SeMPN) was 18.91 ± 1.8 μg/g. SeMPN had a slightly lower molecular weight than MPN, but the carbohydrate content and monosaccharide composition remained identical.
34478790	5	26	located	observed	797:804	arg2	changes					784:790	No significant changes	769:790	No significant changes	769:790	No significant changes were observed in the types and ratios of glycosidic linkages, as determined by NMR spectroscopy.
34478790	5	26	located	observed	797:804	arg1	ratios					823:828	ratios	823:828	ratios	823:828	No significant changes were observed in the types and ratios of glycosidic linkages, as determined by NMR spectroscopy.
34478790	5	26	located	observed	797:804	arg1	types					813:817	types	813:817	types	813:817	No significant changes were observed in the types and ratios of glycosidic linkages, as determined by NMR spectroscopy.
34478790	4	27	from	band	686:689	arg1	MPN					706:708	MPN	706:708	MPN	706:708	Additionally, the band at 606 cm-1 in MPN changed to 615 cm-1 in SeMPN as revealed by FT-IR spectra.
34478790	2	28	theme	lucidum	396:402	arg1	mycelia					404:410	Ganoderma lucidum mycelia	386:410	Ganoderma lucidum mycelia	386:410	The purpose of this research is to determine the influence of selenium on the structure and bioactivity of a polysaccharide fraction (MPN) isolated from Ganoderma lucidum mycelia.
34478790	6	29	from	activity	1134:1141	arg1	cells					1164:1168	H2O2-injured PC12 cells	1146:1168	H2O2-injured PC12 cells	1146:1168	Extracellular and intracellular antioxidant assays demonstrated that SeMPN was more effective than MPN in scavenging free radicals, inhibiting AAPH-induced erythrocyte hemolysis, and protecting catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells.
34478790	6	30	theme	glutathione	1102:1112	arg1	GSH-Px					1126:1131	GSH-Px	1126:1131	GSH-Px	1126:1131	Extracellular and intracellular antioxidant assays demonstrated that SeMPN was more effective than MPN in scavenging free radicals, inhibiting AAPH-induced erythrocyte hemolysis, and protecting catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells.
34478790	6	30	theme	glutathione	1102:1112	arg1	peroxidase					1114:1123	glutathione peroxidase	1102:1123	glutathione peroxidase (GSH-Px)	1102:1132	Extracellular and intracellular antioxidant assays demonstrated that SeMPN was more effective than MPN in scavenging free radicals, inhibiting AAPH-induced erythrocyte hemolysis, and protecting catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells.
34478790	8	31	theme	potential	1346:1354	arg1	supplementation					1376:1390	potential functional selenium supplementation	1346:1390	potential functional selenium supplementation	1346:1390	SeMPN could be as potential functional selenium supplementation.
34478790	6	32	theme	peroxidase	1114:1123	arg1	activity					1134:1141	catalase (CAT) and glutathione peroxidase (GSH-Px) activity	1083:1141	catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells	1083:1168	Extracellular and intracellular antioxidant assays demonstrated that SeMPN was more effective than MPN in scavenging free radicals, inhibiting AAPH-induced erythrocyte hemolysis, and protecting catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells.
34478790	2	33	theme	Ganoderma	386:394	arg1	lucidum					396:402	Ganoderma lucidum	386:402	Ganoderma lucidum mycelia	386:410	The purpose of this research is to determine the influence of selenium on the structure and bioactivity of a polysaccharide fraction (MPN) isolated from Ganoderma lucidum mycelia.
34478790	7	34	theme	higher	1197:1202	arg1	effect					1213:1218	a higher increase effect	1195:1218	a higher increase effect	1195:1218	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	6	35	theme	scavenging	995:1004	arg1	radicals					1011:1018	scavenging free radicals	995:1018	scavenging free radicals	995:1018	Extracellular and intracellular antioxidant assays demonstrated that SeMPN was more effective than MPN in scavenging free radicals, inhibiting AAPH-induced erythrocyte hemolysis, and protecting catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells.
34478790	1	36	theme	dietary	199:205	arg1	supplementation					216:230	the dietary selenium supplementation	195:230	the dietary selenium supplementation	195:230	The development of selenized polysaccharides is a promising strategy for the dietary selenium supplementation.
34478790	7	37	contain	had	1191:1193	arg2	effect					1213:1218	a higher increase effect	1195:1218	a higher increase effect	1195:1218	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	7	37	contain	had	1191:1193	arg1	SeMPN					1185:1189	SeMPN	1185:1189	SeMPN	1185:1189	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	8	38	theme	selenium	1367:1374	arg1	supplementation					1376:1390	potential functional selenium supplementation	1346:1390	potential functional selenium supplementation	1346:1390	SeMPN could be as potential functional selenium supplementation.
34478790	3	39	theme	carbohydrate	596:607	arg1	content					609:615	the carbohydrate content	592:615	the carbohydrate content	592:615	After biological selenium enrichment, the selenium content in the selenized polysaccharide (SeMPN) was 18.91 ± 1.8 μg/g. SeMPN had a slightly lower molecular weight than MPN, but the carbohydrate content and monosaccharide composition remained identical.
34478790	1	40	theme	selenium	207:214	arg1	supplementation					216:230	the dietary selenium supplementation	195:230	the dietary selenium supplementation	195:230	The development of selenized polysaccharides is a promising strategy for the dietary selenium supplementation.
34478790	0	41	theme	comparative	17:27	arg1	study					29:33	comparative study	17:33	comparative study	17:33	Purification and comparative study of bioactivities of a natural selenized polysaccharide from Ganoderma Lucidum mycelia.
34478790	7	42	dep	cells	1233:1237	arg1	capacity					1266:1273	pinocytic and phagocytic capacity	1241:1273	pinocytic and phagocytic capacity	1241:1273	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	7	42	dep	cells	1233:1237	arg1	cells					1233:1237	RAW 264.7 cells	1223:1237	RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6	1223:1325	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	7	42	dep	cells	1233:1237	arg1	production					1293:1302	their production	1287:1302	their production of NO	1287:1308	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	7	42	dep	cells	1233:1237	arg1	TNF-α					1311:1315	TNF-α	1311:1315	TNF-α	1311:1315	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	7	42	dep	cells	1233:1237	arg1	IL-6					1322:1325	IL-6	1322:1325	IL-6	1322:1325	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	3	43	theme	18.91 ± 1.8 μg/g.	516:532	arg1	SeMPN					534:538	18.91 ± 1.8 μg/g. SeMPN	516:538	18.91 ± 1.8 μg/g. SeMPN	516:538	After biological selenium enrichment, the selenium content in the selenized polysaccharide (SeMPN) was 18.91 ± 1.8 μg/g. SeMPN had a slightly lower molecular weight than MPN, but the carbohydrate content and monosaccharide composition remained identical.
34478790	3	44	theme	selenized	479:487	arg1	SeMPN					505:509	SeMPN	505:509	SeMPN	505:509	After biological selenium enrichment, the selenium content in the selenized polysaccharide (SeMPN) was 18.91 ± 1.8 μg/g. SeMPN had a slightly lower molecular weight than MPN, but the carbohydrate content and monosaccharide composition remained identical.
34478790	3	44	theme	selenized	479:487	arg1	polysaccharide					489:502	the selenized polysaccharide	475:502	the selenized polysaccharide (SeMPN)	475:510	After biological selenium enrichment, the selenium content in the selenized polysaccharide (SeMPN) was 18.91 ± 1.8 μg/g. SeMPN had a slightly lower molecular weight than MPN, but the carbohydrate content and monosaccharide composition remained identical.
34478790	0	45	theme	bioactivities	38:50	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and comparative study of bioactivities of a natural selenized polysaccharide from Ganoderma Lucidum mycelia.
34478790	0	45	theme	bioactivities	38:50	arg1	study					29:33	comparative study	17:33	comparative study	17:33	Purification and comparative study of bioactivities of a natural selenized polysaccharide from Ganoderma Lucidum mycelia.
34478790	6	46	theme	H2O2-injured	1146:1157	arg1	cells					1164:1168	H2O2-injured PC12 cells	1146:1168	H2O2-injured PC12 cells	1146:1168	Extracellular and intracellular antioxidant assays demonstrated that SeMPN was more effective than MPN in scavenging free radicals, inhibiting AAPH-induced erythrocyte hemolysis, and protecting catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells.
34478790	2	47	theme	research	253:260	arg1	purpose					237:243	The purpose	233:243	The purpose of this research	233:260	The purpose of this research is to determine the influence of selenium on the structure and bioactivity of a polysaccharide fraction (MPN) isolated from Ganoderma lucidum mycelia.
34478790	3	48	theme	selenium	430:437	arg1	enrichment					439:448	biological selenium enrichment	419:448	biological selenium enrichment	419:448	After biological selenium enrichment, the selenium content in the selenized polysaccharide (SeMPN) was 18.91 ± 1.8 μg/g. SeMPN had a slightly lower molecular weight than MPN, but the carbohydrate content and monosaccharide composition remained identical.
34478790	3	49	from	content	464:470	arg1	SeMPN					505:509	SeMPN	505:509	SeMPN	505:509	After biological selenium enrichment, the selenium content in the selenized polysaccharide (SeMPN) was 18.91 ± 1.8 μg/g. SeMPN had a slightly lower molecular weight than MPN, but the carbohydrate content and monosaccharide composition remained identical.
34478790	3	49	from	content	464:470	arg1	polysaccharide					489:502	the selenized polysaccharide	475:502	the selenized polysaccharide (SeMPN)	475:510	After biological selenium enrichment, the selenium content in the selenized polysaccharide (SeMPN) was 18.91 ± 1.8 μg/g. SeMPN had a slightly lower molecular weight than MPN, but the carbohydrate content and monosaccharide composition remained identical.
34478790	7	50	theme	pinocytic	1241:1249	arg1	capacity					1266:1273	pinocytic and phagocytic capacity	1241:1273	pinocytic and phagocytic capacity	1241:1273	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	7	50	theme	pinocytic	1241:1249	arg1	cells					1233:1237	RAW 264.7 cells	1223:1237	RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6	1223:1325	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	6	51	theme	Extracellular	889:901	arg1	assays					933:938	Extracellular and intracellular antioxidant assays	889:938	Extracellular and intracellular antioxidant assays	889:938	Extracellular and intracellular antioxidant assays demonstrated that SeMPN was more effective than MPN in scavenging free radicals, inhibiting AAPH-induced erythrocyte hemolysis, and protecting catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells.
34478790	2	52	theme	fraction	357:364	arg1	structure					311:319	structure	311:319	structure	311:319	The purpose of this research is to determine the influence of selenium on the structure and bioactivity of a polysaccharide fraction (MPN) isolated from Ganoderma lucidum mycelia.
34478790	2	52	theme	fraction	357:364	arg1	bioactivity					325:335	bioactivity	325:335	bioactivity	325:335	The purpose of this research is to determine the influence of selenium on the structure and bioactivity of a polysaccharide fraction (MPN) isolated from Ganoderma lucidum mycelia.
34478790	7	53	theme	RAW	1223:1225	arg1	capacity					1266:1273	pinocytic and phagocytic capacity	1241:1273	pinocytic and phagocytic capacity	1241:1273	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	7	53	theme	RAW	1223:1225	arg1	cells					1233:1237	RAW 264.7 cells	1223:1237	RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6	1223:1325	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	7	53	theme	RAW	1223:1225	arg1	production					1293:1302	their production	1287:1302	their production of NO	1287:1308	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	7	53	theme	RAW	1223:1225	arg1	TNF-α					1311:1315	TNF-α	1311:1315	TNF-α	1311:1315	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	7	53	theme	RAW	1223:1225	arg1	IL-6					1322:1325	IL-6	1322:1325	IL-6	1322:1325	Additionally, SeMPN had a higher increase effect on RAW 264.7 cells's pinocytic and phagocytic capacity, as well as their production of NO, TNF-α, and IL-6.
34478790	2	54	theme	polysaccharide	342:355	arg1	MPN					367:369	MPN	367:369	MPN	367:369	The purpose of this research is to determine the influence of selenium on the structure and bioactivity of a polysaccharide fraction (MPN) isolated from Ganoderma lucidum mycelia.
34478790	2	54	theme	polysaccharide	342:355	arg1	fraction					357:364	a polysaccharide fraction	340:364	a polysaccharide fraction (MPN) isolated from Ganoderma lucidum mycelia	340:410	The purpose of this research is to determine the influence of selenium on the structure and bioactivity of a polysaccharide fraction (MPN) isolated from Ganoderma lucidum mycelia.
34478790	6	55	from	effective	973:981	arg1	radicals					1011:1018	scavenging free radicals	995:1018	scavenging free radicals	995:1018	Extracellular and intracellular antioxidant assays demonstrated that SeMPN was more effective than MPN in scavenging free radicals, inhibiting AAPH-induced erythrocyte hemolysis, and protecting catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells.
34478790	0	56	from	mycelia	113:119	arg1	polysaccharide					75:88	a natural selenized polysaccharide	55:88	a natural selenized polysaccharide from Ganoderma Lucidum mycelia	55:119	Purification and comparative study of bioactivities of a natural selenized polysaccharide from Ganoderma Lucidum mycelia.
34478790	0	56	from	mycelia	113:119	arg1	bioactivities					38:50	bioactivities	38:50	bioactivities of a natural selenized polysaccharide from Ganoderma Lucidum mycelia	38:119	Purification and comparative study of bioactivities of a natural selenized polysaccharide from Ganoderma Lucidum mycelia.
34478790	2	57	from	influence	282:290	arg1	structure					311:319	structure	311:319	structure	311:319	The purpose of this research is to determine the influence of selenium on the structure and bioactivity of a polysaccharide fraction (MPN) isolated from Ganoderma lucidum mycelia.
34478790	2	57	from	influence	282:290	arg1	bioactivity					325:335	bioactivity	325:335	bioactivity	325:335	The purpose of this research is to determine the influence of selenium on the structure and bioactivity of a polysaccharide fraction (MPN) isolated from Ganoderma lucidum mycelia.
34478790	4	58	theme	FT-IR	754:758	arg1	spectra					760:766	FT-IR spectra	754:766	FT-IR spectra	754:766	Additionally, the band at 606 cm-1 in MPN changed to 615 cm-1 in SeMPN as revealed by FT-IR spectra.
34478790	1	59	theme	selenized	141:149	arg1	polysaccharides					151:165	selenized polysaccharides	141:165	selenized polysaccharides	141:165	The development of selenized polysaccharides is a promising strategy for the dietary selenium supplementation.
34478790	6	60	theme	PC12	1159:1162	arg1	cells					1164:1168	H2O2-injured PC12 cells	1146:1168	H2O2-injured PC12 cells	1146:1168	Extracellular and intracellular antioxidant assays demonstrated that SeMPN was more effective than MPN in scavenging free radicals, inhibiting AAPH-induced erythrocyte hemolysis, and protecting catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells.
34478790	0	61	theme	selenized	65:73	arg1	polysaccharide					75:88	a natural selenized polysaccharide	55:88	a natural selenized polysaccharide from Ganoderma Lucidum mycelia	55:119	Purification and comparative study of bioactivities of a natural selenized polysaccharide from Ganoderma Lucidum mycelia.
34478790	5	62	theme	glycosidic	833:842	arg1	linkages					844:851	glycosidic linkages	833:851	glycosidic linkages	833:851	No significant changes were observed in the types and ratios of glycosidic linkages, as determined by NMR spectroscopy.
34478790	1	63	theme	polysaccharides	151:165	arg1	strategy					182:189	a promising strategy	170:189	a promising strategy for the dietary selenium supplementation	170:230	The development of selenized polysaccharides is a promising strategy for the dietary selenium supplementation.
34478790	1	63	theme	polysaccharides	151:165	arg1	development					126:136	The development	122:136	The development of selenized polysaccharides	122:165	The development of selenized polysaccharides is a promising strategy for the dietary selenium supplementation.
34478790	3	64	theme	selenium	455:462	arg1	content					464:470	the selenium content	451:470	the selenium content in the selenized polysaccharide (SeMPN)	451:510	After biological selenium enrichment, the selenium content in the selenized polysaccharide (SeMPN) was 18.91 ± 1.8 μg/g. SeMPN had a slightly lower molecular weight than MPN, but the carbohydrate content and monosaccharide composition remained identical.
34478790	6	65	theme	erythrocyte	1045:1055	arg1	hemolysis					1057:1065	AAPH-induced erythrocyte hemolysis	1032:1065	AAPH-induced erythrocyte hemolysis	1032:1065	Extracellular and intracellular antioxidant assays demonstrated that SeMPN was more effective than MPN in scavenging free radicals, inhibiting AAPH-induced erythrocyte hemolysis, and protecting catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells.
34478790	0	66	theme	natural	57:63	arg1	polysaccharide					75:88	a natural selenized polysaccharide	55:88	a natural selenized polysaccharide from Ganoderma Lucidum mycelia	55:119	Purification and comparative study of bioactivities of a natural selenized polysaccharide from Ganoderma Lucidum mycelia.
34478790	6	67	dep	radicals	1011:1018	arg1	inhibiting					1021:1030	inhibiting	1021:1030	inhibiting AAPH-induced erythrocyte hemolysis	1021:1065	Extracellular and intracellular antioxidant assays demonstrated that SeMPN was more effective than MPN in scavenging free radicals, inhibiting AAPH-induced erythrocyte hemolysis, and protecting catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells.
34478790	6	67	dep	radicals	1011:1018	arg1	protecting					1072:1081	protecting	1072:1081	protecting catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells	1072:1168	Extracellular and intracellular antioxidant assays demonstrated that SeMPN was more effective than MPN in scavenging free radicals, inhibiting AAPH-induced erythrocyte hemolysis, and protecting catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells.
34478790	3	68	theme	monosaccharide	621:634	arg1	composition					636:646	monosaccharide composition	621:646	monosaccharide composition	621:646	After biological selenium enrichment, the selenium content in the selenized polysaccharide (SeMPN) was 18.91 ± 1.8 μg/g. SeMPN had a slightly lower molecular weight than MPN, but the carbohydrate content and monosaccharide composition remained identical.
34478790	6	69	theme	AAPH-induced	1032:1043	arg1	hemolysis					1057:1065	AAPH-induced erythrocyte hemolysis	1032:1065	AAPH-induced erythrocyte hemolysis	1032:1065	Extracellular and intracellular antioxidant assays demonstrated that SeMPN was more effective than MPN in scavenging free radicals, inhibiting AAPH-induced erythrocyte hemolysis, and protecting catalase (CAT) and glutathione peroxidase (GSH-Px) activity in H2O2-injured PC12 cells.
32561278	4	0	theme	phosphoric	877:886	arg1	dissolution					902:912	cold phosphoric acid-mediated dissolution	872:912	cold phosphoric acid-mediated dissolution of microcrystalline cellulose (MCC)	872:948	RC with sphere-like shape was successfully prepared using a green method based on cold phosphoric acid-mediated dissolution of microcrystalline cellulose (MCC) followed by regeneration process using water as anti-solvent.
32561278	8	1	theme	low	1814:1816	arg1	content					1821:1827	low RC content	1814:1827	low RC content (i.e. RC weight fraction of 2.5 or 5 wt%)	1814:1869	Collected results reveal that CHF with low RC content (i.e. RC weight fraction of 2.5 or 5 wt%) have the best tensile and swelling properties, with a tensile strength and a swelling capacity (at pH = 6.4) up to 95 MPa and 4000%, respectively.
32561278	3	2	theme	hydroxyethyl	705:716	arg1	HEC					729:731	HEC	729:731	HEC	729:731	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	3	2	theme	hydroxyethyl	705:716	arg1	cellulose					718:726	hydroxyethyl cellulose	705:726	hydroxyethyl cellulose (HEC)	705:732	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	6	3	theme	solvent	1248:1254	arg1	method					1264:1269	solvent casting method	1248:1269	solvent casting method	1248:1269	CHF crosslinked with citric acid were, in fact, prepared by solvent casting method with different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%), then the crosslinking reaction was triggered by thermal treatment at 80 °C during 8 h. Prepared CHF were then characterized in terms of their structural, thermal, tensile and transparency properties.
32561278	3	4	theme	superabsorbent	539:552	arg1	CHF					580:582	CHF	580:582	CHF	580:582	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	3	4	theme	superabsorbent	539:552	arg1	films					573:577	cross-linked superabsorbent composite hydrogel films	526:577	cross-linked superabsorbent composite hydrogel films (CHF)	526:583	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	7	5	theme	hydrogel	1676:1683	arg1	films					1685:1689	hydrogel films	1676:1689	hydrogel films	1676:1689	Swelling tests were carried at three different aqueous media (i.e. with a pH = 3, 6.4 or 11) to evaluate the water retention capacity of hydrogel films, as well as, the pH effect on their swelling and hydrolytic degradation properties.
32561278	0	6	theme	Phosphoric	0:9	arg1	preparation					31:41	Phosphoric acid-mediated green preparation	0:41	Phosphoric acid-mediated green preparation of regenerated cellulose spheres and their use for all-cellulose cross-linked superabsorbent hydrogels.	0:145	Phosphoric acid-mediated green preparation of regenerated cellulose spheres and their use for all-cellulose cross-linked superabsorbent hydrogels.
32561278	4	7	with	RC	790:791	arg1	shape					810:814	sphere-like shape	798:814	sphere-like shape	798:814	RC with sphere-like shape was successfully prepared using a green method based on cold phosphoric acid-mediated dissolution of microcrystalline cellulose (MCC) followed by regeneration process using water as anti-solvent.
32561278	3	8	link	cross-linked	526:537	arg1	CHF					580:582	CHF	580:582	CHF	580:582	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	3	8	link	cross-linked	526:537	arg1	films					573:577	cross-linked superabsorbent composite hydrogel films	526:577	cross-linked superabsorbent composite hydrogel films (CHF)	526:583	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	7	9	theme	water	1648:1652	arg1	capacity					1664:1671	the water retention capacity	1644:1671	the water retention capacity of hydrogel films	1644:1689	Swelling tests were carried at three different aqueous media (i.e. with a pH = 3, 6.4 or 11) to evaluate the water retention capacity of hydrogel films, as well as, the pH effect on their swelling and hydrolytic degradation properties.
32561278	3	10	theme	regenerated	754:764	arg1	spheres					781:787	newly developed regenerated cellulose (RC) spheres	738:787	newly developed regenerated cellulose (RC) spheres	738:787	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	2	11	theme	cellulose	336:344	arg1	derivatives					346:356	cellulose derivatives	336:356	cellulose derivatives	336:356	Hydrogels based on cellulose derivatives can absorb and retain a huge amount of water in the interstitial sites of their structures, stimulating their uses in various useful industrial purposes.
32561278	6	12	theme	weight	1289:1294	arg1	fractions					1296:1304	different RC weight fractions	1276:1304	different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%)	1276:1336	CHF crosslinked with citric acid were, in fact, prepared by solvent casting method with different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%), then the crosslinking reaction was triggered by thermal treatment at 80 °C during 8 h. Prepared CHF were then characterized in terms of their structural, thermal, tensile and transparency properties.
32561278	6	12	theme	weight	1289:1294	arg1	%					1335:1335	0, 2.5, 5, 10 and 15 wt%	1312:1335	0, 2.5, 5, 10 and 15 wt%	1312:1335	CHF crosslinked with citric acid were, in fact, prepared by solvent casting method with different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%), then the crosslinking reaction was triggered by thermal treatment at 80 °C during 8 h. Prepared CHF were then characterized in terms of their structural, thermal, tensile and transparency properties.
32561278	7	13	theme	Swelling	1539:1546	arg1	tests					1548:1552	Swelling tests	1539:1552	Swelling tests	1539:1552	Swelling tests were carried at three different aqueous media (i.e. with a pH = 3, 6.4 or 11) to evaluate the water retention capacity of hydrogel films, as well as, the pH effect on their swelling and hydrolytic degradation properties.
32561278	4	14	theme	microcrystalline	917:932	arg1	cellulose					934:942	microcrystalline cellulose	917:942	microcrystalline cellulose (MCC)	917:948	RC with sphere-like shape was successfully prepared using a green method based on cold phosphoric acid-mediated dissolution of microcrystalline cellulose (MCC) followed by regeneration process using water as anti-solvent.
32561278	4	14	theme	microcrystalline	917:932	arg1	MCC					945:947	MCC	945:947	MCC	945:947	RC with sphere-like shape was successfully prepared using a green method based on cold phosphoric acid-mediated dissolution of microcrystalline cellulose (MCC) followed by regeneration process using water as anti-solvent.
32561278	6	15	theme	citric	1209:1214	arg1	acid					1216:1219	citric acid	1209:1219	citric acid	1209:1219	CHF crosslinked with citric acid were, in fact, prepared by solvent casting method with different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%), then the crosslinking reaction was triggered by thermal treatment at 80 °C during 8 h. Prepared CHF were then characterized in terms of their structural, thermal, tensile and transparency properties.
32561278	6	16	theme	crosslinking	1348:1359	arg1	reaction					1361:1368	then the crosslinking reaction	1339:1368	then the crosslinking reaction	1339:1368	CHF crosslinked with citric acid were, in fact, prepared by solvent casting method with different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%), then the crosslinking reaction was triggered by thermal treatment at 80 °C during 8 h. Prepared CHF were then characterized in terms of their structural, thermal, tensile and transparency properties.
32561278	6	17	theme	different	1276:1284	arg1	fractions					1296:1304	different RC weight fractions	1276:1304	different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%)	1276:1336	CHF crosslinked with citric acid were, in fact, prepared by solvent casting method with different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%), then the crosslinking reaction was triggered by thermal treatment at 80 °C during 8 h. Prepared CHF were then characterized in terms of their structural, thermal, tensile and transparency properties.
32561278	6	17	theme	different	1276:1284	arg1	%					1335:1335	0, 2.5, 5, 10 and 15 wt%	1312:1335	0, 2.5, 5, 10 and 15 wt%	1312:1335	CHF crosslinked with citric acid were, in fact, prepared by solvent casting method with different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%), then the crosslinking reaction was triggered by thermal treatment at 80 °C during 8 h. Prepared CHF were then characterized in terms of their structural, thermal, tensile and transparency properties.
32561278	8	18	theme	RC	1818:1819	arg1	content					1821:1827	low RC content	1814:1827	low RC content (i.e. RC weight fraction of 2.5 or 5 wt%)	1814:1869	Collected results reveal that CHF with low RC content (i.e. RC weight fraction of 2.5 or 5 wt%) have the best tensile and swelling properties, with a tensile strength and a swelling capacity (at pH = 6.4) up to 95 MPa and 4000%, respectively.
32561278	5	19	theme	RC	1077:1078	arg1	spheres					1080:1086	RC spheres	1077:1086	RC spheres	1077:1086	Prior to be used, the morphological and structural properties of RC spheres, with an average diameter of 477 ± 270 nm, were examined by SEM, AFM, XRD, FTIR and TGA techniques.
32561278	0	20	theme	use	86:88	arg1	preparation					31:41	Phosphoric acid-mediated green preparation	0:41	Phosphoric acid-mediated green preparation of regenerated cellulose spheres and their use for all-cellulose cross-linked superabsorbent hydrogels.	0:145	Phosphoric acid-mediated green preparation of regenerated cellulose spheres and their use for all-cellulose cross-linked superabsorbent hydrogels.
32561278	7	21	dep	carried	1559:1565	arg1	i.e.					1601:1604	i.e.	1601:1604	i.e.	1601:1604	Swelling tests were carried at three different aqueous media (i.e. with a pH = 3, 6.4 or 11) to evaluate the water retention capacity of hydrogel films, as well as, the pH effect on their swelling and hydrolytic degradation properties.
32561278	2	22	dep	useful	484:489	arg1	industrial					491:500	industrial	491:500	industrial	491:500	Hydrogels based on cellulose derivatives can absorb and retain a huge amount of water in the interstitial sites of their structures, stimulating their uses in various useful industrial purposes.
32561278	8	23	dep	fraction	1845:1852	arg1	i.e.					1830:1833	i.e.	1830:1833	i.e.	1830:1833	Collected results reveal that CHF with low RC content (i.e. RC weight fraction of 2.5 or 5 wt%) have the best tensile and swelling properties, with a tensile strength and a swelling capacity (at pH = 6.4) up to 95 MPa and 4000%, respectively.
32561278	8	24	dep	95 MPa	1986:1991	arg1	up					1980:1981	up	1980:1981	up	1980:1981	Collected results reveal that CHF with low RC content (i.e. RC weight fraction of 2.5 or 5 wt%) have the best tensile and swelling properties, with a tensile strength and a swelling capacity (at pH = 6.4) up to 95 MPa and 4000%, respectively.
32561278	0	25	theme	all-cellulose	94:106	arg1	hydrogels					136:144	all-cellulose cross-linked superabsorbent hydrogels	94:144	all-cellulose cross-linked superabsorbent hydrogels	94:144	Phosphoric acid-mediated green preparation of regenerated cellulose spheres and their use for all-cellulose cross-linked superabsorbent hydrogels.
32561278	5	26	with	spheres	1080:1086	arg1	diameter					1105:1112	an average diameter	1094:1112	an average diameter of 477 ± 270 nm	1094:1128	Prior to be used, the morphological and structural properties of RC spheres, with an average diameter of 477 ± 270 nm, were examined by SEM, AFM, XRD, FTIR and TGA techniques.
32561278	0	27	theme	superabsorbent	121:134	arg1	hydrogels					136:144	all-cellulose cross-linked superabsorbent hydrogels	94:144	all-cellulose cross-linked superabsorbent hydrogels	94:144	Phosphoric acid-mediated green preparation of regenerated cellulose spheres and their use for all-cellulose cross-linked superabsorbent hydrogels.
32561278	5	28	theme	477 ± 270 nm	1117:1128	arg1	diameter					1105:1112	an average diameter	1094:1112	an average diameter of 477 ± 270 nm	1094:1128	Prior to be used, the morphological and structural properties of RC spheres, with an average diameter of 477 ± 270 nm, were examined by SEM, AFM, XRD, FTIR and TGA techniques.
32561278	2	29	dep	their	462:466	arg1	uses					468:471	uses	468:471	uses	468:471	Hydrogels based on cellulose derivatives can absorb and retain a huge amount of water in the interstitial sites of their structures, stimulating their uses in various useful industrial purposes.
32561278	8	30	theme	swelling	1897:1904	arg1	properties					1906:1915	the best tensile and swelling properties	1876:1915	the best tensile and swelling properties	1876:1915	Collected results reveal that CHF with low RC content (i.e. RC weight fraction of 2.5 or 5 wt%) have the best tensile and swelling properties, with a tensile strength and a swelling capacity (at pH = 6.4) up to 95 MPa and 4000%, respectively.
32561278	2	31	theme	various	476:482	arg1	purposes					502:509	various useful industrial purposes	476:509	various useful industrial purposes	476:509	Hydrogels based on cellulose derivatives can absorb and retain a huge amount of water in the interstitial sites of their structures, stimulating their uses in various useful industrial purposes.
32561278	2	32	theme	interstitial	410:421	arg1	sites					423:427	the interstitial sites	406:427	the interstitial sites of their structures	406:447	Hydrogels based on cellulose derivatives can absorb and retain a huge amount of water in the interstitial sites of their structures, stimulating their uses in various useful industrial purposes.
32561278	2	32	theme	interstitial	410:421	arg1	structures					438:447	their structures	432:447	their structures	432:447	Hydrogels based on cellulose derivatives can absorb and retain a huge amount of water in the interstitial sites of their structures, stimulating their uses in various useful industrial purposes.
32561278	4	33	dep	based	863:867	arg1	followed					950:957	followed	950:957	followed by regeneration process using water as anti-solvent	950:1009	RC with sphere-like shape was successfully prepared using a green method based on cold phosphoric acid-mediated dissolution of microcrystalline cellulose (MCC) followed by regeneration process using water as anti-solvent.
32561278	4	33	dep	based	863:867	arg1	method					856:861	a green method	848:861	a green method based on cold phosphoric acid-mediated dissolution of microcrystalline cellulose (MCC) followed by regeneration process using water as anti-solvent	848:1009	RC with sphere-like shape was successfully prepared using a green method based on cold phosphoric acid-mediated dissolution of microcrystalline cellulose (MCC) followed by regeneration process using water as anti-solvent.
32561278	6	34	theme	properties	1527:1536	arg1	terms					1466:1470	terms	1466:1470	terms of their structural, thermal, tensile and transparency properties	1466:1536	CHF crosslinked with citric acid were, in fact, prepared by solvent casting method with different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%), then the crosslinking reaction was triggered by thermal treatment at 80 °C during 8 h. Prepared CHF were then characterized in terms of their structural, thermal, tensile and transparency properties.
32561278	7	35	theme	aqueous	1586:1592	arg1	media					1594:1598	three different aqueous media	1570:1598	three different aqueous media	1570:1598	Swelling tests were carried at three different aqueous media (i.e. with a pH = 3, 6.4 or 11) to evaluate the water retention capacity of hydrogel films, as well as, the pH effect on their swelling and hydrolytic degradation properties.
32561278	0	36	theme	green	25:29	arg1	preparation					31:41	Phosphoric acid-mediated green preparation	0:41	Phosphoric acid-mediated green preparation of regenerated cellulose spheres and their use for all-cellulose cross-linked superabsorbent hydrogels.	0:145	Phosphoric acid-mediated green preparation of regenerated cellulose spheres and their use for all-cellulose cross-linked superabsorbent hydrogels.
32561278	3	37	theme	cellulose	688:696	arg1	combination					659:669	the combination	655:669	the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres	655:787	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	2	38	theme	structures	438:447	arg1	sites					423:427	the interstitial sites	406:427	the interstitial sites of their structures	406:447	Hydrogels based on cellulose derivatives can absorb and retain a huge amount of water in the interstitial sites of their structures, stimulating their uses in various useful industrial purposes.
32561278	2	38	theme	structures	438:447	arg1	structures					438:447	their structures	432:447	their structures	432:447	Hydrogels based on cellulose derivatives can absorb and retain a huge amount of water in the interstitial sites of their structures, stimulating their uses in various useful industrial purposes.
32561278	5	39	theme	morphological	1034:1046	arg1	properties					1063:1072	the morphological and structural properties	1030:1072	the morphological and structural properties of RC spheres, with an average diameter of 477 ± 270 nm,	1030:1129	Prior to be used, the morphological and structural properties of RC spheres, with an average diameter of 477 ± 270 nm, were examined by SEM, AFM, XRD, FTIR and TGA techniques.
32561278	2	40	theme	huge	382:385	arg1	water					397:401	water	397:401	water	397:401	Hydrogels based on cellulose derivatives can absorb and retain a huge amount of water in the interstitial sites of their structures, stimulating their uses in various useful industrial purposes.
32561278	2	40	theme	huge	382:385	arg1	amount					387:392	a huge amount	380:392	a huge amount of water	380:401	Hydrogels based on cellulose derivatives can absorb and retain a huge amount of water in the interstitial sites of their structures, stimulating their uses in various useful industrial purposes.
32561278	0	41	theme	cellulose	58:66	arg1	spheres					68:74	regenerated cellulose spheres	46:74	regenerated cellulose spheres	46:74	Phosphoric acid-mediated green preparation of regenerated cellulose spheres and their use for all-cellulose cross-linked superabsorbent hydrogels.
32561278	7	42	theme	hydrolytic	1740:1749	arg1	properties					1763:1772	their swelling and hydrolytic degradation properties	1721:1772	their swelling and hydrolytic degradation properties	1721:1772	Swelling tests were carried at three different aqueous media (i.e. with a pH = 3, 6.4 or 11) to evaluate the water retention capacity of hydrogel films, as well as, the pH effect on their swelling and hydrolytic degradation properties.
32561278	6	43	from	80 °C	1408:1412	arg1	treatment					1395:1403	thermal treatment	1387:1403	thermal treatment at 80 °C	1387:1412	CHF crosslinked with citric acid were, in fact, prepared by solvent casting method with different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%), then the crosslinking reaction was triggered by thermal treatment at 80 °C during 8 h. Prepared CHF were then characterized in terms of their structural, thermal, tensile and transparency properties.
32561278	4	44	theme	sphere-like	798:808	arg1	shape					810:814	sphere-like shape	798:814	sphere-like shape	798:814	RC with sphere-like shape was successfully prepared using a green method based on cold phosphoric acid-mediated dissolution of microcrystalline cellulose (MCC) followed by regeneration process using water as anti-solvent.
32561278	7	45	theme	swelling	1727:1734	arg1	properties					1763:1772	their swelling and hydrolytic degradation properties	1721:1772	their swelling and hydrolytic degradation properties	1721:1772	Swelling tests were carried at three different aqueous media (i.e. with a pH = 3, 6.4 or 11) to evaluate the water retention capacity of hydrogel films, as well as, the pH effect on their swelling and hydrolytic degradation properties.
32561278	8	46	contain	have	1871:1874	arg1	CHF					1805:1807	CHF	1805:1807	CHF with low RC content (i.e. RC weight fraction of 2.5 or 5 wt%)	1805:1869	Collected results reveal that CHF with low RC content (i.e. RC weight fraction of 2.5 or 5 wt%) have the best tensile and swelling properties, with a tensile strength and a swelling capacity (at pH = 6.4) up to 95 MPa and 4000%, respectively.
32561278	8	46	contain	have	1871:1874	arg2	properties					1906:1915	the best tensile and swelling properties	1876:1915	the best tensile and swelling properties	1876:1915	Collected results reveal that CHF with low RC content (i.e. RC weight fraction of 2.5 or 5 wt%) have the best tensile and swelling properties, with a tensile strength and a swelling capacity (at pH = 6.4) up to 95 MPa and 4000%, respectively.
32561278	3	47	theme	composite	554:562	arg1	CHF					580:582	CHF	580:582	CHF	580:582	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	3	47	theme	composite	554:562	arg1	films					573:577	cross-linked superabsorbent composite hydrogel films	526:577	cross-linked superabsorbent composite hydrogel films (CHF)	526:583	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	1	48	theme	superabsorbent	257:270	arg1	hydrogels					272:280	eco-friendly superabsorbent hydrogels	244:280	eco-friendly superabsorbent hydrogels instead of synthetic counterparts	244:314	With the growing environmental concerns and an emergent demand, a growing attention is turned to eco-friendly superabsorbent hydrogels instead of synthetic counterparts.
32561278	0	49	link	cross-linked	108:119	arg1	hydrogels					136:144	all-cellulose cross-linked superabsorbent hydrogels	94:144	all-cellulose cross-linked superabsorbent hydrogels	94:144	Phosphoric acid-mediated green preparation of regenerated cellulose spheres and their use for all-cellulose cross-linked superabsorbent hydrogels.
32561278	6	50	theme	casting	1256:1262	arg1	method					1264:1269	solvent casting method	1248:1269	solvent casting method	1248:1269	CHF crosslinked with citric acid were, in fact, prepared by solvent casting method with different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%), then the crosslinking reaction was triggered by thermal treatment at 80 °C during 8 h. Prepared CHF were then characterized in terms of their structural, thermal, tensile and transparency properties.
32561278	3	51	theme	cross-linked	526:537	arg1	CHF					580:582	CHF	580:582	CHF	580:582	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	3	51	theme	cross-linked	526:537	arg1	films					573:577	cross-linked superabsorbent composite hydrogel films	526:577	cross-linked superabsorbent composite hydrogel films (CHF)	526:583	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	4	52	theme	cold	872:875	arg1	dissolution					902:912	cold phosphoric acid-mediated dissolution	872:912	cold phosphoric acid-mediated dissolution of microcrystalline cellulose (MCC)	872:948	RC with sphere-like shape was successfully prepared using a green method based on cold phosphoric acid-mediated dissolution of microcrystalline cellulose (MCC) followed by regeneration process using water as anti-solvent.
32561278	7	53	theme	films	1685:1689	arg1	capacity					1664:1671	the water retention capacity	1644:1671	the water retention capacity of hydrogel films	1644:1689	Swelling tests were carried at three different aqueous media (i.e. with a pH = 3, 6.4 or 11) to evaluate the water retention capacity of hydrogel films, as well as, the pH effect on their swelling and hydrolytic degradation properties.
32561278	3	54	theme	cellulose	718:726	arg1	combination					659:669	the combination	655:669	the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres	655:787	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	1	55	theme	synthetic	293:301	arg1	counterparts					303:314	synthetic counterparts	293:314	eco-friendly superabsorbent hydrogels instead of synthetic counterparts	244:314	With the growing environmental concerns and an emergent demand, a growing attention is turned to eco-friendly superabsorbent hydrogels instead of synthetic counterparts.
32561278	4	56	theme	acid-mediated	888:900	arg1	dissolution					902:912	cold phosphoric acid-mediated dissolution	872:912	cold phosphoric acid-mediated dissolution of microcrystalline cellulose (MCC)	872:948	RC with sphere-like shape was successfully prepared using a green method based on cold phosphoric acid-mediated dissolution of microcrystalline cellulose (MCC) followed by regeneration process using water as anti-solvent.
32561278	3	57	theme	developed	744:752	arg1	spheres					781:787	newly developed regenerated cellulose (RC) spheres	738:787	newly developed regenerated cellulose (RC) spheres	738:787	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	1	58	theme	emergent	194:201	arg1	demand					203:208	an emergent demand	191:208	an emergent demand	191:208	With the growing environmental concerns and an emergent demand, a growing attention is turned to eco-friendly superabsorbent hydrogels instead of synthetic counterparts.
32561278	8	59	theme	tensile	1885:1891	arg1	properties					1906:1915	the best tensile and swelling properties	1876:1915	the best tensile and swelling properties	1876:1915	Collected results reveal that CHF with low RC content (i.e. RC weight fraction of 2.5 or 5 wt%) have the best tensile and swelling properties, with a tensile strength and a swelling capacity (at pH = 6.4) up to 95 MPa and 4000%, respectively.
32561278	4	60	theme	green	850:854	arg1	followed					950:957	followed	950:957	followed by regeneration process using water as anti-solvent	950:1009	RC with sphere-like shape was successfully prepared using a green method based on cold phosphoric acid-mediated dissolution of microcrystalline cellulose (MCC) followed by regeneration process using water as anti-solvent.
32561278	4	60	theme	green	850:854	arg1	method					856:861	a green method	848:861	a green method based on cold phosphoric acid-mediated dissolution of microcrystalline cellulose (MCC) followed by regeneration process using water as anti-solvent	848:1009	RC with sphere-like shape was successfully prepared using a green method based on cold phosphoric acid-mediated dissolution of microcrystalline cellulose (MCC) followed by regeneration process using water as anti-solvent.
32561278	7	61	theme	retention	1654:1662	arg1	capacity					1664:1671	the water retention capacity	1644:1671	the water retention capacity of hydrogel films	1644:1689	Swelling tests were carried at three different aqueous media (i.e. with a pH = 3, 6.4 or 11) to evaluate the water retention capacity of hydrogel films, as well as, the pH effect on their swelling and hydrolytic degradation properties.
32561278	8	62	theme	RC	1835:1836	arg1	fraction					1845:1852	RC weight fraction	1835:1852	RC weight fraction of 2.5 or 5 wt%	1835:1868	Collected results reveal that CHF with low RC content (i.e. RC weight fraction of 2.5 or 5 wt%) have the best tensile and swelling properties, with a tensile strength and a swelling capacity (at pH = 6.4) up to 95 MPa and 4000%, respectively.
32561278	8	63	theme	Collected	1775:1783	arg1	results					1785:1791	Collected results	1775:1791	Collected results	1775:1791	Collected results reveal that CHF with low RC content (i.e. RC weight fraction of 2.5 or 5 wt%) have the best tensile and swelling properties, with a tensile strength and a swelling capacity (at pH = 6.4) up to 95 MPa and 4000%, respectively.
32561278	3	64	theme	RC	777:778	arg1	spheres					781:787	newly developed regenerated cellulose (RC) spheres	738:787	newly developed regenerated cellulose (RC) spheres	738:787	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	5	65	theme	structural	1052:1061	arg1	properties					1063:1072	the morphological and structural properties	1030:1072	the morphological and structural properties of RC spheres, with an average diameter of 477 ± 270 nm,	1030:1129	Prior to be used, the morphological and structural properties of RC spheres, with an average diameter of 477 ± 270 nm, were examined by SEM, AFM, XRD, FTIR and TGA techniques.
32561278	1	66	theme	growing	213:219	arg1	attention					221:229	a growing attention	211:229	a growing attention	211:229	With the growing environmental concerns and an emergent demand, a growing attention is turned to eco-friendly superabsorbent hydrogels instead of synthetic counterparts.
32561278	3	67	theme	cellulose	766:774	arg1	spheres					781:787	newly developed regenerated cellulose (RC) spheres	738:787	newly developed regenerated cellulose (RC) spheres	738:787	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	6	68	theme	RC	1286:1287	arg1	fractions					1296:1304	different RC weight fractions	1276:1304	different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%)	1276:1336	CHF crosslinked with citric acid were, in fact, prepared by solvent casting method with different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%), then the crosslinking reaction was triggered by thermal treatment at 80 °C during 8 h. Prepared CHF were then characterized in terms of their structural, thermal, tensile and transparency properties.
32561278	6	68	theme	RC	1286:1287	arg1	%					1335:1335	0, 2.5, 5, 10 and 15 wt%	1312:1335	0, 2.5, 5, 10 and 15 wt%	1312:1335	CHF crosslinked with citric acid were, in fact, prepared by solvent casting method with different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%), then the crosslinking reaction was triggered by thermal treatment at 80 °C during 8 h. Prepared CHF were then characterized in terms of their structural, thermal, tensile and transparency properties.
32561278	5	69	theme	average	1097:1103	arg1	diameter					1105:1112	an average diameter	1094:1112	an average diameter of 477 ± 270 nm	1094:1128	Prior to be used, the morphological and structural properties of RC spheres, with an average diameter of 477 ± 270 nm, were examined by SEM, AFM, XRD, FTIR and TGA techniques.
32561278	6	70	theme	tensile	1502:1508	arg1	properties					1527:1536	their structural, thermal, tensile and transparency properties	1475:1536	their structural, thermal, tensile and transparency properties	1475:1536	CHF crosslinked with citric acid were, in fact, prepared by solvent casting method with different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%), then the crosslinking reaction was triggered by thermal treatment at 80 °C during 8 h. Prepared CHF were then characterized in terms of their structural, thermal, tensile and transparency properties.
32561278	8	71	with	CHF	1805:1807	arg1	content					1821:1827	low RC content	1814:1827	low RC content (i.e. RC weight fraction of 2.5 or 5 wt%)	1814:1869	Collected results reveal that CHF with low RC content (i.e. RC weight fraction of 2.5 or 5 wt%) have the best tensile and swelling properties, with a tensile strength and a swelling capacity (at pH = 6.4) up to 95 MPa and 4000%, respectively.
32561278	3	72	theme	spheres	781:787	arg1	combination					659:669	the combination	655:669	the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres	655:787	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	6	73	theme	thermal	1493:1499	arg1	properties					1527:1536	their structural, thermal, tensile and transparency properties	1475:1536	their structural, thermal, tensile and transparency properties	1475:1536	CHF crosslinked with citric acid were, in fact, prepared by solvent casting method with different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%), then the crosslinking reaction was triggered by thermal treatment at 80 °C during 8 h. Prepared CHF were then characterized in terms of their structural, thermal, tensile and transparency properties.
32561278	4	74	theme	cellulose	934:942	arg1	dissolution					902:912	cold phosphoric acid-mediated dissolution	872:912	cold phosphoric acid-mediated dissolution of microcrystalline cellulose (MCC)	872:948	RC with sphere-like shape was successfully prepared using a green method based on cold phosphoric acid-mediated dissolution of microcrystalline cellulose (MCC) followed by regeneration process using water as anti-solvent.
32561278	8	75	theme	%	1868:1868	arg1	fraction					1845:1852	RC weight fraction	1835:1852	RC weight fraction of 2.5 or 5 wt%	1835:1868	Collected results reveal that CHF with low RC content (i.e. RC weight fraction of 2.5 or 5 wt%) have the best tensile and swelling properties, with a tensile strength and a swelling capacity (at pH = 6.4) up to 95 MPa and 4000%, respectively.
32561278	5	76	theme	spheres	1080:1086	arg1	properties					1063:1072	the morphological and structural properties	1030:1072	the morphological and structural properties of RC spheres, with an average diameter of 477 ± 270 nm,	1030:1129	Prior to be used, the morphological and structural properties of RC spheres, with an average diameter of 477 ± 270 nm, were examined by SEM, AFM, XRD, FTIR and TGA techniques.
32561278	6	77	theme	structural	1481:1490	arg1	properties					1527:1536	their structural, thermal, tensile and transparency properties	1475:1536	their structural, thermal, tensile and transparency properties	1475:1536	CHF crosslinked with citric acid were, in fact, prepared by solvent casting method with different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%), then the crosslinking reaction was triggered by thermal treatment at 80 °C during 8 h. Prepared CHF were then characterized in terms of their structural, thermal, tensile and transparency properties.
32561278	8	78	theme	weight	1838:1843	arg1	fraction					1845:1852	RC weight fraction	1835:1852	RC weight fraction of 2.5 or 5 wt%	1835:1868	Collected results reveal that CHF with low RC content (i.e. RC weight fraction of 2.5 or 5 wt%) have the best tensile and swelling properties, with a tensile strength and a swelling capacity (at pH = 6.4) up to 95 MPa and 4000%, respectively.
32561278	0	79	theme	cross-linked	108:119	arg1	hydrogels					136:144	all-cellulose cross-linked superabsorbent hydrogels	94:144	all-cellulose cross-linked superabsorbent hydrogels	94:144	Phosphoric acid-mediated green preparation of regenerated cellulose spheres and their use for all-cellulose cross-linked superabsorbent hydrogels.
32561278	5	80	theme	SEM	1148:1150	arg1	techniques					1176:1185	SEM, AFM, XRD, FTIR and TGA techniques	1148:1185	SEM, AFM, XRD, FTIR and TGA techniques	1148:1185	Prior to be used, the morphological and structural properties of RC spheres, with an average diameter of 477 ± 270 nm, were examined by SEM, AFM, XRD, FTIR and TGA techniques.
32561278	4	81	theme	regeneration	962:973	arg1	process					975:981	regeneration process	962:981	regeneration process	962:981	RC with sphere-like shape was successfully prepared using a green method based on cold phosphoric acid-mediated dissolution of microcrystalline cellulose (MCC) followed by regeneration process using water as anti-solvent.
32561278	8	82	dep	content	1821:1827	arg1	fraction					1845:1852	RC weight fraction	1835:1852	RC weight fraction of 2.5 or 5 wt%	1835:1868	Collected results reveal that CHF with low RC content (i.e. RC weight fraction of 2.5 or 5 wt%) have the best tensile and swelling properties, with a tensile strength and a swelling capacity (at pH = 6.4) up to 95 MPa and 4000%, respectively.
32561278	0	83	theme	acid-mediated	11:23	arg1	preparation					31:41	Phosphoric acid-mediated green preparation	0:41	Phosphoric acid-mediated green preparation of regenerated cellulose spheres and their use for all-cellulose cross-linked superabsorbent hydrogels.	0:145	Phosphoric acid-mediated green preparation of regenerated cellulose spheres and their use for all-cellulose cross-linked superabsorbent hydrogels.
32561278	5	84	theme	FTIR	1163:1166	arg1	techniques					1176:1185	SEM, AFM, XRD, FTIR and TGA techniques	1148:1185	SEM, AFM, XRD, FTIR and TGA techniques	1148:1185	Prior to be used, the morphological and structural properties of RC spheres, with an average diameter of 477 ± 270 nm, were examined by SEM, AFM, XRD, FTIR and TGA techniques.
32561278	6	85	dep	%	1335:1335	arg1	i.e.					1307:1310	i.e.	1307:1310	i.e.	1307:1310	CHF crosslinked with citric acid were, in fact, prepared by solvent casting method with different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%), then the crosslinking reaction was triggered by thermal treatment at 80 °C during 8 h. Prepared CHF were then characterized in terms of their structural, thermal, tensile and transparency properties.
32561278	6	86	theme	transparency	1514:1525	arg1	properties					1527:1536	their structural, thermal, tensile and transparency properties	1475:1536	their structural, thermal, tensile and transparency properties	1475:1536	CHF crosslinked with citric acid were, in fact, prepared by solvent casting method with different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%), then the crosslinking reaction was triggered by thermal treatment at 80 °C during 8 h. Prepared CHF were then characterized in terms of their structural, thermal, tensile and transparency properties.
32561278	8	87	theme	tensile	1925:1931	arg1	strength					1933:1940	a tensile strength	1923:1940	a tensile strength	1923:1940	Collected results reveal that CHF with low RC content (i.e. RC weight fraction of 2.5 or 5 wt%) have the best tensile and swelling properties, with a tensile strength and a swelling capacity (at pH = 6.4) up to 95 MPa and 4000%, respectively.
32561278	3	88	theme	carboxymethyl	674:686	arg1	CMC					699:701	CMC	699:701	CMC	699:701	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	3	88	theme	carboxymethyl	674:686	arg1	cellulose					688:696	carboxymethyl cellulose	674:696	carboxymethyl cellulose (CMC)	674:702	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	0	89	theme	regenerated	46:56	arg1	spheres					68:74	regenerated cellulose spheres	46:74	regenerated cellulose spheres	46:74	Phosphoric acid-mediated green preparation of regenerated cellulose spheres and their use for all-cellulose cross-linked superabsorbent hydrogels.
32561278	5	90	theme	TGA	1172:1174	arg1	techniques					1176:1185	SEM, AFM, XRD, FTIR and TGA techniques	1148:1185	SEM, AFM, XRD, FTIR and TGA techniques	1148:1185	Prior to be used, the morphological and structural properties of RC spheres, with an average diameter of 477 ± 270 nm, were examined by SEM, AFM, XRD, FTIR and TGA techniques.
32561278	7	91	theme	degradation	1751:1761	arg1	properties					1763:1772	their swelling and hydrolytic degradation properties	1721:1772	their swelling and hydrolytic degradation properties	1721:1772	Swelling tests were carried at three different aqueous media (i.e. with a pH = 3, 6.4 or 11) to evaluate the water retention capacity of hydrogel films, as well as, the pH effect on their swelling and hydrolytic degradation properties.
32561278	7	92	theme	different	1576:1584	arg1	media					1594:1598	three different aqueous media	1570:1598	three different aqueous media	1570:1598	Swelling tests were carried at three different aqueous media (i.e. with a pH = 3, 6.4 or 11) to evaluate the water retention capacity of hydrogel films, as well as, the pH effect on their swelling and hydrolytic degradation properties.
32561278	7	93	from	effect	1711:1716	arg1	properties					1763:1772	their swelling and hydrolytic degradation properties	1721:1772	their swelling and hydrolytic degradation properties	1721:1772	Swelling tests were carried at three different aqueous media (i.e. with a pH = 3, 6.4 or 11) to evaluate the water retention capacity of hydrogel films, as well as, the pH effect on their swelling and hydrolytic degradation properties.
32561278	3	94	theme	combination	659:669	arg1	advantage					642:650	advantage	642:650	advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres	642:787	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	0	95	theme	spheres	68:74	arg1	preparation					31:41	Phosphoric acid-mediated green preparation	0:41	Phosphoric acid-mediated green preparation of regenerated cellulose spheres and their use for all-cellulose cross-linked superabsorbent hydrogels.	0:145	Phosphoric acid-mediated green preparation of regenerated cellulose spheres and their use for all-cellulose cross-linked superabsorbent hydrogels.
32561278	5	96	theme	AFM	1153:1155	arg1	techniques					1176:1185	SEM, AFM, XRD, FTIR and TGA techniques	1148:1185	SEM, AFM, XRD, FTIR and TGA techniques	1148:1185	Prior to be used, the morphological and structural properties of RC spheres, with an average diameter of 477 ± 270 nm, were examined by SEM, AFM, XRD, FTIR and TGA techniques.
32561278	8	97	theme	swelling	1948:1955	arg1	capacity					1957:1964	a swelling capacity	1946:1964	a swelling capacity (at pH = 6.4)	1946:1978	Collected results reveal that CHF with low RC content (i.e. RC weight fraction of 2.5 or 5 wt%) have the best tensile and swelling properties, with a tensile strength and a swelling capacity (at pH = 6.4) up to 95 MPa and 4000%, respectively.
32561278	8	97	theme	swelling	1948:1955	arg1	at					1967:1968	at pH = 6.4	1967:1977	at pH = 6.4	1967:1977	Collected results reveal that CHF with low RC content (i.e. RC weight fraction of 2.5 or 5 wt%) have the best tensile and swelling properties, with a tensile strength and a swelling capacity (at pH = 6.4) up to 95 MPa and 4000%, respectively.
32561278	2	98	theme	useful	484:489	arg1	purposes					502:509	various useful industrial purposes	476:509	various useful industrial purposes	476:509	Hydrogels based on cellulose derivatives can absorb and retain a huge amount of water in the interstitial sites of their structures, stimulating their uses in various useful industrial purposes.
32561278	5	99	theme	XRD	1158:1160	arg1	techniques					1176:1185	SEM, AFM, XRD, FTIR and TGA techniques	1148:1185	SEM, AFM, XRD, FTIR and TGA techniques	1148:1185	Prior to be used, the morphological and structural properties of RC spheres, with an average diameter of 477 ± 270 nm, were examined by SEM, AFM, XRD, FTIR and TGA techniques.
32561278	7	100	theme	pH	1708:1709	arg1	effect					1711:1716	the pH effect	1704:1716	the pH effect on their swelling and hydrolytic degradation properties	1704:1772	Swelling tests were carried at three different aqueous media (i.e. with a pH = 3, 6.4 or 11) to evaluate the water retention capacity of hydrogel films, as well as, the pH effect on their swelling and hydrolytic degradation properties.
32561278	2	101	theme	water	397:401	arg1	water					397:401	water	397:401	water	397:401	Hydrogels based on cellulose derivatives can absorb and retain a huge amount of water in the interstitial sites of their structures, stimulating their uses in various useful industrial purposes.
32561278	2	101	theme	water	397:401	arg1	amount					387:392	a huge amount	380:392	a huge amount of water	380:401	Hydrogels based on cellulose derivatives can absorb and retain a huge amount of water in the interstitial sites of their structures, stimulating their uses in various useful industrial purposes.
32561278	1	102	theme	eco-friendly	244:255	arg1	hydrogels					272:280	eco-friendly superabsorbent hydrogels	244:280	eco-friendly superabsorbent hydrogels instead of synthetic counterparts	244:314	With the growing environmental concerns and an emergent demand, a growing attention is turned to eco-friendly superabsorbent hydrogels instead of synthetic counterparts.
32561278	3	103	theme	hydrogel	564:571	arg1	CHF					580:582	CHF	580:582	CHF	580:582	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	3	103	theme	hydrogel	564:571	arg1	films					573:577	cross-linked superabsorbent composite hydrogel films	526:577	cross-linked superabsorbent composite hydrogel films (CHF)	526:583	In this work, cross-linked superabsorbent composite hydrogel films (CHF) were designed, manufactured and characterized, by taking advantage of the combination of carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC) and newly developed regenerated cellulose (RC) spheres.
32561278	6	104	theme	thermal	1387:1393	arg1	treatment					1395:1403	thermal treatment	1387:1403	thermal treatment at 80 °C	1387:1412	CHF crosslinked with citric acid were, in fact, prepared by solvent casting method with different RC weight fractions (i.e. 0, 2.5, 5, 10 and 15 wt%), then the crosslinking reaction was triggered by thermal treatment at 80 °C during 8 h. Prepared CHF were then characterized in terms of their structural, thermal, tensile and transparency properties.
33357923	0	0	theme	composite	121:129	arg1	hydrogels					131:139	free-standing carboxylated cellulose nanofiber/metal composite hydrogels	68:139	free-standing carboxylated cellulose nanofiber/metal composite hydrogels	68:139	One-step electrochemically induced counterion exchange to construct free-standing carboxylated cellulose nanofiber/metal composite hydrogels.
33357923	6	1	theme	composite	1102:1110	arg1	hydrogels					1112:1120	nanofiber/metal composite hydrogels	1086:1120	nanofiber/metal composite hydrogels	1086:1120	The results suggest great potential of anodic electrodeposition to fabricate nanofiber/metal composite hydrogels.
33357923	3	2	theme	cellulose	617:625	arg1	nanofibers					627:636	carboxylated cellulose nanofibers	604:636	carboxylated cellulose nanofibers	604:636	Metal ions were electrochemically and locally released from the electrode and chelated with carboxylated cellulose nanofibers, leading to the in-situ formation of composite hydrogels.
33357923	1	3	theme	manufacturing	300:312	arg1	nanomaterials					314:326	manufacturing nanomaterials	300:326	manufacturing nanomaterials	300:326	The fabrication of polymeric composite hydrogel with hierarchical structure in a simple, controllable, and straightforward process poses great importance for manufacturing nanomaterials and subsequent applications.
33357923	0	4	theme	nanofiber/metal	105:119	arg1	hydrogels					131:139	free-standing carboxylated cellulose nanofiber/metal composite hydrogels	68:139	free-standing carboxylated cellulose nanofiber/metal composite hydrogels	68:139	One-step electrochemically induced counterion exchange to construct free-standing carboxylated cellulose nanofiber/metal composite hydrogels.
33357923	5	5	theme	enhanced	922:929	arg1	degradation					939:949	enhanced thermal degradation	922:949	enhanced thermal degradation	922:949	Significantly, the composited hydrogels exhibited interconnected nanoporous structure, enhanced thermal degradation, improved mechanical strength and antibacterial activity.
33357923	2	6	theme	template-free	390:402	arg1	method					424:429	a one-step and template-free counterion exchange method	375:429	a one-step and template-free counterion exchange method to construct free-standing carboxylated cellulose nanofiber composite hydrogels	375:509	Herein, we report a one-step and template-free counterion exchange method to construct free-standing carboxylated cellulose nanofiber composite hydrogels.
33357923	3	7	theme	in-situ	654:660	arg1	formation					662:670	the in-situ formation	650:670	the in-situ formation of composite hydrogels	650:693	Metal ions were electrochemically and locally released from the electrode and chelated with carboxylated cellulose nanofibers, leading to the in-situ formation of composite hydrogels.
33357923	3	8	theme	composite	675:683	arg1	hydrogels					685:693	composite hydrogels	675:693	composite hydrogels	675:693	Metal ions were electrochemically and locally released from the electrode and chelated with carboxylated cellulose nanofibers, leading to the in-situ formation of composite hydrogels.
33357923	4	9	theme	composite	714:722	arg1	hydrogels					724:732	composite hydrogels	714:732	composite hydrogels	714:732	The properties of composite hydrogels can be easily programmed by the type of electrode, current density, and electrodeposited suspension.
33357923	3	10	theme	hydrogels	685:693	arg1	formation					662:670	the in-situ formation	650:670	the in-situ formation of composite hydrogels	650:693	Metal ions were electrochemically and locally released from the electrode and chelated with carboxylated cellulose nanofibers, leading to the in-situ formation of composite hydrogels.
33357923	4	11	theme	electrodeposited	806:821	arg1	suspension					823:832	electrodeposited suspension	806:832	electrodeposited suspension	806:832	The properties of composite hydrogels can be easily programmed by the type of electrode, current density, and electrodeposited suspension.
33357923	1	12	theme	subsequent	332:341	arg1	applications					343:354	subsequent applications	332:354	subsequent applications	332:354	The fabrication of polymeric composite hydrogel with hierarchical structure in a simple, controllable, and straightforward process poses great importance for manufacturing nanomaterials and subsequent applications.
33357923	6	13	theme	electrodeposition	1055:1071	arg1	potential					1035:1043	great potential	1029:1043	great potential of anodic electrodeposition to fabricate nanofiber/metal composite hydrogels	1029:1120	The results suggest great potential of anodic electrodeposition to fabricate nanofiber/metal composite hydrogels.
33357923	3	14	attach	released	558:565	arg2	ions					518:521	Metal ions	512:521	Metal ions	512:521	Metal ions were electrochemically and locally released from the electrode and chelated with carboxylated cellulose nanofibers, leading to the in-situ formation of composite hydrogels.
33357923	3	14	attach	released	558:565	arg1	electrode					576:584	the electrode	572:584	the electrode	572:584	Metal ions were electrochemically and locally released from the electrode and chelated with carboxylated cellulose nanofibers, leading to the in-situ formation of composite hydrogels.
33357923	4	15	theme	current	785:791	arg1	density					793:799	current density	785:799	current density	785:799	The properties of composite hydrogels can be easily programmed by the type of electrode, current density, and electrodeposited suspension.
33357923	2	16	theme	composite	491:499	arg1	hydrogels					501:509	free-standing carboxylated cellulose nanofiber composite hydrogels	444:509	free-standing carboxylated cellulose nanofiber composite hydrogels	444:509	Herein, we report a one-step and template-free counterion exchange method to construct free-standing carboxylated cellulose nanofiber composite hydrogels.
33357923	6	17	theme	anodic	1048:1053	arg1	electrodeposition					1055:1071	anodic electrodeposition	1048:1071	anodic electrodeposition	1048:1071	The results suggest great potential of anodic electrodeposition to fabricate nanofiber/metal composite hydrogels.
33357923	1	18	with	hydrogel	181:188	arg1	structure					208:216	hierarchical structure	195:216	hierarchical structure	195:216	The fabrication of polymeric composite hydrogel with hierarchical structure in a simple, controllable, and straightforward process poses great importance for manufacturing nanomaterials and subsequent applications.
33357923	4	19	theme	hydrogels	724:732	arg1	properties					700:709	The properties	696:709	The properties of composite hydrogels	696:732	The properties of composite hydrogels can be easily programmed by the type of electrode, current density, and electrodeposited suspension.
33357923	1	20	theme	simple	223:228	arg1	process					265:271	a simple, controllable, and straightforward process	221:271	a simple, controllable, and straightforward process	221:271	The fabrication of polymeric composite hydrogel with hierarchical structure in a simple, controllable, and straightforward process poses great importance for manufacturing nanomaterials and subsequent applications.
33357923	5	21	theme	improved	952:959	arg1	strength					972:979	improved mechanical strength	952:979	improved mechanical strength	952:979	Significantly, the composited hydrogels exhibited interconnected nanoporous structure, enhanced thermal degradation, improved mechanical strength and antibacterial activity.
33357923	5	22	theme	interconnected	885:898	arg1	structure					911:919	interconnected nanoporous structure	885:919	interconnected nanoporous structure	885:919	Significantly, the composited hydrogels exhibited interconnected nanoporous structure, enhanced thermal degradation, improved mechanical strength and antibacterial activity.
33357923	2	23	theme	one-step	377:384	arg1	method					424:429	a one-step and template-free counterion exchange method	375:429	a one-step and template-free counterion exchange method to construct free-standing carboxylated cellulose nanofiber composite hydrogels	375:509	Herein, we report a one-step and template-free counterion exchange method to construct free-standing carboxylated cellulose nanofiber composite hydrogels.
33357923	2	24	theme	cellulose	471:479	arg1	hydrogels					501:509	free-standing carboxylated cellulose nanofiber composite hydrogels	444:509	free-standing carboxylated cellulose nanofiber composite hydrogels	444:509	Herein, we report a one-step and template-free counterion exchange method to construct free-standing carboxylated cellulose nanofiber composite hydrogels.
33357923	0	25	theme	One-step	0:7	arg1	exchange					46:53	One-step electrochemically induced counterion exchange	0:53	One-step electrochemically induced counterion exchange	0:53	One-step electrochemically induced counterion exchange to construct free-standing carboxylated cellulose nanofiber/metal composite hydrogels.
33357923	1	26	theme	controllable	231:242	arg1	process					265:271	a simple, controllable, and straightforward process	221:271	a simple, controllable, and straightforward process	221:271	The fabrication of polymeric composite hydrogel with hierarchical structure in a simple, controllable, and straightforward process poses great importance for manufacturing nanomaterials and subsequent applications.
33357923	0	27	theme	counterion	35:44	arg1	exchange					46:53	One-step electrochemically induced counterion exchange	0:53	One-step electrochemically induced counterion exchange	0:53	One-step electrochemically induced counterion exchange to construct free-standing carboxylated cellulose nanofiber/metal composite hydrogels.
33357923	5	28	theme	mechanical	961:970	arg1	strength					972:979	improved mechanical strength	952:979	improved mechanical strength	952:979	Significantly, the composited hydrogels exhibited interconnected nanoporous structure, enhanced thermal degradation, improved mechanical strength and antibacterial activity.
33357923	5	29	theme	antibacterial	985:997	arg1	activity					999:1006	antibacterial activity	985:1006	antibacterial activity	985:1006	Significantly, the composited hydrogels exhibited interconnected nanoporous structure, enhanced thermal degradation, improved mechanical strength and antibacterial activity.
33357923	0	30	theme	induced	27:33	arg1	exchange					46:53	One-step electrochemically induced counterion exchange	0:53	One-step electrochemically induced counterion exchange	0:53	One-step electrochemically induced counterion exchange to construct free-standing carboxylated cellulose nanofiber/metal composite hydrogels.
33357923	3	31	theme	carboxylated	604:615	arg1	nanofibers					627:636	carboxylated cellulose nanofibers	604:636	carboxylated cellulose nanofibers	604:636	Metal ions were electrochemically and locally released from the electrode and chelated with carboxylated cellulose nanofibers, leading to the in-situ formation of composite hydrogels.
33357923	3	32	theme	Metal	512:516	arg1	ions					518:521	Metal ions	512:521	Metal ions	512:521	Metal ions were electrochemically and locally released from the electrode and chelated with carboxylated cellulose nanofibers, leading to the in-situ formation of composite hydrogels.
33357923	2	33	theme	counterion	404:413	arg1	method					424:429	a one-step and template-free counterion exchange method	375:429	a one-step and template-free counterion exchange method to construct free-standing carboxylated cellulose nanofiber composite hydrogels	375:509	Herein, we report a one-step and template-free counterion exchange method to construct free-standing carboxylated cellulose nanofiber composite hydrogels.
33357923	6	34	theme	great	1029:1033	arg1	potential					1035:1043	great potential	1029:1043	great potential of anodic electrodeposition to fabricate nanofiber/metal composite hydrogels	1029:1120	The results suggest great potential of anodic electrodeposition to fabricate nanofiber/metal composite hydrogels.
33357923	2	35	theme	carboxylated	458:469	arg1	hydrogels					501:509	free-standing carboxylated cellulose nanofiber composite hydrogels	444:509	free-standing carboxylated cellulose nanofiber composite hydrogels	444:509	Herein, we report a one-step and template-free counterion exchange method to construct free-standing carboxylated cellulose nanofiber composite hydrogels.
33357923	1	36	theme	straightforward	249:263	arg1	process					265:271	a simple, controllable, and straightforward process	221:271	a simple, controllable, and straightforward process	221:271	The fabrication of polymeric composite hydrogel with hierarchical structure in a simple, controllable, and straightforward process poses great importance for manufacturing nanomaterials and subsequent applications.
33357923	4	37	theme	electrode	774:782	arg1	type					766:769	the type	762:769	the type of electrode, current density, and electrodeposited suspension	762:832	The properties of composite hydrogels can be easily programmed by the type of electrode, current density, and electrodeposited suspension.
33357923	5	38	theme	thermal	931:937	arg1	degradation					939:949	enhanced thermal degradation	922:949	enhanced thermal degradation	922:949	Significantly, the composited hydrogels exhibited interconnected nanoporous structure, enhanced thermal degradation, improved mechanical strength and antibacterial activity.
33357923	5	39	theme	composited	854:863	arg1	hydrogels					865:873	the composited hydrogels	850:873	the composited hydrogels	850:873	Significantly, the composited hydrogels exhibited interconnected nanoporous structure, enhanced thermal degradation, improved mechanical strength and antibacterial activity.
33357923	0	40	theme	free-standing	68:80	arg1	hydrogels					131:139	free-standing carboxylated cellulose nanofiber/metal composite hydrogels	68:139	free-standing carboxylated cellulose nanofiber/metal composite hydrogels	68:139	One-step electrochemically induced counterion exchange to construct free-standing carboxylated cellulose nanofiber/metal composite hydrogels.
33357923	5	41	theme	nanoporous	900:909	arg1	structure					911:919	interconnected nanoporous structure	885:919	interconnected nanoporous structure	885:919	Significantly, the composited hydrogels exhibited interconnected nanoporous structure, enhanced thermal degradation, improved mechanical strength and antibacterial activity.
33357923	2	42	theme	exchange	415:422	arg1	method					424:429	a one-step and template-free counterion exchange method	375:429	a one-step and template-free counterion exchange method to construct free-standing carboxylated cellulose nanofiber composite hydrogels	375:509	Herein, we report a one-step and template-free counterion exchange method to construct free-standing carboxylated cellulose nanofiber composite hydrogels.
33357923	6	43	theme	nanofiber/metal	1086:1100	arg1	hydrogels					1112:1120	nanofiber/metal composite hydrogels	1086:1120	nanofiber/metal composite hydrogels	1086:1120	The results suggest great potential of anodic electrodeposition to fabricate nanofiber/metal composite hydrogels.
33357923	1	44	theme	polymeric	161:169	arg1	hydrogel					181:188	polymeric composite hydrogel	161:188	polymeric composite hydrogel with hierarchical structure	161:216	The fabrication of polymeric composite hydrogel with hierarchical structure in a simple, controllable, and straightforward process poses great importance for manufacturing nanomaterials and subsequent applications.
33357923	4	45	theme	density	793:799	arg1	type					766:769	the type	762:769	the type of electrode, current density, and electrodeposited suspension	762:832	The properties of composite hydrogels can be easily programmed by the type of electrode, current density, and electrodeposited suspension.
33357923	1	46	theme	composite	171:179	arg1	hydrogel					181:188	polymeric composite hydrogel	161:188	polymeric composite hydrogel with hierarchical structure	161:216	The fabrication of polymeric composite hydrogel with hierarchical structure in a simple, controllable, and straightforward process poses great importance for manufacturing nanomaterials and subsequent applications.
33357923	1	47	from	fabrication	146:156	arg1	process					265:271	a simple, controllable, and straightforward process	221:271	a simple, controllable, and straightforward process	221:271	The fabrication of polymeric composite hydrogel with hierarchical structure in a simple, controllable, and straightforward process poses great importance for manufacturing nanomaterials and subsequent applications.
33357923	1	48	theme	hydrogel	181:188	arg1	fabrication					146:156	The fabrication	142:156	The fabrication of polymeric composite hydrogel with hierarchical structure in a simple, controllable, and straightforward process	142:271	The fabrication of polymeric composite hydrogel with hierarchical structure in a simple, controllable, and straightforward process poses great importance for manufacturing nanomaterials and subsequent applications.
33357923	1	49	theme	great	279:283	arg1	importance					285:294	great importance	279:294	great importance for manufacturing nanomaterials and subsequent applications	279:354	The fabrication of polymeric composite hydrogel with hierarchical structure in a simple, controllable, and straightforward process poses great importance for manufacturing nanomaterials and subsequent applications.
33357923	0	50	theme	cellulose	95:103	arg1	hydrogels					131:139	free-standing carboxylated cellulose nanofiber/metal composite hydrogels	68:139	free-standing carboxylated cellulose nanofiber/metal composite hydrogels	68:139	One-step electrochemically induced counterion exchange to construct free-standing carboxylated cellulose nanofiber/metal composite hydrogels.
33357923	2	51	theme	free-standing	444:456	arg1	hydrogels					501:509	free-standing carboxylated cellulose nanofiber composite hydrogels	444:509	free-standing carboxylated cellulose nanofiber composite hydrogels	444:509	Herein, we report a one-step and template-free counterion exchange method to construct free-standing carboxylated cellulose nanofiber composite hydrogels.
33357923	2	52	theme	nanofiber	481:489	arg1	hydrogels					501:509	free-standing carboxylated cellulose nanofiber composite hydrogels	444:509	free-standing carboxylated cellulose nanofiber composite hydrogels	444:509	Herein, we report a one-step and template-free counterion exchange method to construct free-standing carboxylated cellulose nanofiber composite hydrogels.
33357923	0	53	theme	carboxylated	82:93	arg1	hydrogels					131:139	free-standing carboxylated cellulose nanofiber/metal composite hydrogels	68:139	free-standing carboxylated cellulose nanofiber/metal composite hydrogels	68:139	One-step electrochemically induced counterion exchange to construct free-standing carboxylated cellulose nanofiber/metal composite hydrogels.
33357923	1	54	theme	hierarchical	195:206	arg1	structure					208:216	hierarchical structure	195:216	hierarchical structure	195:216	The fabrication of polymeric composite hydrogel with hierarchical structure in a simple, controllable, and straightforward process poses great importance for manufacturing nanomaterials and subsequent applications.
33357923	4	55	theme	suspension	823:832	arg1	type					766:769	the type	762:769	the type of electrode, current density, and electrodeposited suspension	762:832	The properties of composite hydrogels can be easily programmed by the type of electrode, current density, and electrodeposited suspension.
33401230	4	0	theme	trap/time-of-flight	881:899	arg1	LC-IT-TOF/MS					920:931	LC-IT-TOF/MS	920:931	LC-IT-TOF/MS	920:931	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	4	0	theme	trap/time-of-flight	881:899	arg1	spectrometry					906:917	high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry	838:917	high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis	838:941	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	3	1	theme	heparin	495:501	arg1	library					519:525	a heparin oligosaccharide library	493:525	a heparin oligosaccharide library	493:525	In this study, a heparin oligosaccharide library, including dp2, dp4 and dp6, were prepared from the chemical modification of the fully sulfated dp2, dp4 and dp6.
33401230	2	2	from	role	466:469	arg1	HS					474:475	HS	474:475	HS	474:475	Therefore, the ability to chemically generate a series of oligosaccharides, which have a similar structure to the naturally-occurring, 2-O- and 6-O-sulfating oligosaccharides from HS, would greatly contribute to investigating their natural role in HS.
33401230	4	3	theme	chromatography-ion	862:879	arg1	LC-IT-TOF/MS					920:931	LC-IT-TOF/MS	920:931	LC-IT-TOF/MS	920:931	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	4	3	theme	chromatography-ion	862:879	arg1	spectrometry					906:917	high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry	838:917	high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis	838:941	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	3	4	theme	oligosaccharide	503:517	arg1	library					519:525	a heparin oligosaccharide library	493:525	a heparin oligosaccharide library	493:525	In this study, a heparin oligosaccharide library, including dp2, dp4 and dp6, were prepared from the chemical modification of the fully sulfated dp2, dp4 and dp6.
33401230	4	5	dep	composition	781:791	arg1	the					764:766	the	764:766	the	764:766	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	4	6	theme	spectrometry	906:917	arg1	analysis					934:941	high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis	838:941	high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis	838:941	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	5	7	theme	groups	1031:1036	arg1	studies					1009:1015	further structural and functional studies	975:1015	further structural and functional studies of O-sulfated groups in HS	975:1042	This provides a foundation for further structural and functional studies of O-sulfated groups in HS.
33401230	4	8	theme	2-O-	719:722	arg1	oligosaccharides					741:756	2-O- and 6-O-sulfated oligosaccharides	719:756	2-O- and 6-O-sulfated oligosaccharides	719:756	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	4	9	theme	mass	901:904	arg1	LC-IT-TOF/MS					920:931	LC-IT-TOF/MS	920:931	LC-IT-TOF/MS	920:931	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	4	9	theme	mass	901:904	arg1	spectrometry					906:917	high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry	838:917	high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis	838:941	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	2	10	theme	oligosaccharides	284:299	arg1	series					274:279	a series	272:279	a series of oligosaccharides, which have a similar structure to the naturally-occurring, 2-O- and 6-O-sulfating oligosaccharides from HS,	272:408	Therefore, the ability to chemically generate a series of oligosaccharides, which have a similar structure to the naturally-occurring, 2-O- and 6-O-sulfating oligosaccharides from HS, would greatly contribute to investigating their natural role in HS.
33401230	4	11	theme	Chemical	641:648	arg1	conditions					659:668	Chemical reaction conditions	641:668	Chemical reaction conditions	641:668	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	4	12	theme	liquid	855:860	arg1	LC-IT-TOF/MS					920:931	LC-IT-TOF/MS	920:931	LC-IT-TOF/MS	920:931	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	4	12	theme	liquid	855:860	arg1	spectrometry					906:917	high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry	838:917	high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis	838:941	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	2	13	theme	similar	315:321	arg1	structure					323:331	a similar structure	313:331	a similar structure	313:331	Therefore, the ability to chemically generate a series of oligosaccharides, which have a similar structure to the naturally-occurring, 2-O- and 6-O-sulfating oligosaccharides from HS, would greatly contribute to investigating their natural role in HS.
33401230	5	14	from	groups	1031:1036	arg1	HS					1041:1042	HS	1041:1042	HS	1041:1042	This provides a foundation for further structural and functional studies of O-sulfated groups in HS.
33401230	1	15	theme	important	175:183	arg1	roles					219:223	important biological and pathophysiological roles	175:223	important biological and pathophysiological roles	175:223	The O-sulfation, including 2-O- and 6-O-sulfation, in heparan sulfate (HS) have important biological and pathophysiological roles.
33401230	2	16	theme	natural	458:464	arg1	role					466:469	their natural role	452:469	their natural role in HS	452:475	Therefore, the ability to chemically generate a series of oligosaccharides, which have a similar structure to the naturally-occurring, 2-O- and 6-O-sulfating oligosaccharides from HS, would greatly contribute to investigating their natural role in HS.
33401230	4	17	theme	high-performance	838:853	arg1	LC-IT-TOF/MS					920:931	LC-IT-TOF/MS	920:931	LC-IT-TOF/MS	920:931	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	4	17	theme	high-performance	838:853	arg1	spectrometry					906:917	high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry	838:917	high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis	838:941	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	1	18	theme	biological	185:194	arg1	roles					219:223	important biological and pathophysiological roles	175:223	important biological and pathophysiological roles	175:223	The O-sulfation, including 2-O- and 6-O-sulfation, in heparan sulfate (HS) have important biological and pathophysiological roles.
33401230	4	19	theme	6-O-sulfated	728:739	arg1	oligosaccharides					741:756	2-O- and 6-O-sulfated oligosaccharides	719:756	2-O- and 6-O-sulfated oligosaccharides	719:756	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	4	20	theme	oligosaccharides	741:756	arg1	patterns					707:714	different patterns	697:714	different patterns of 2-O- and 6-O-sulfated oligosaccharides	697:756	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	5	21	theme	structural	983:992	arg1	studies					1009:1015	further structural and functional studies	975:1015	further structural and functional studies of O-sulfated groups in HS	975:1042	This provides a foundation for further structural and functional studies of O-sulfated groups in HS.
33401230	1	22	theme	pathophysiological	200:217	arg1	roles					219:223	important biological and pathophysiological roles	175:223	important biological and pathophysiological roles	175:223	The O-sulfation, including 2-O- and 6-O-sulfation, in heparan sulfate (HS) have important biological and pathophysiological roles.
33401230	0	23	theme	de-O-sulfation	44:57	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of partial de-O-sulfation of heparin oligosaccharide library.
33401230	0	23	theme	de-O-sulfation	44:57	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of partial de-O-sulfation of heparin oligosaccharide library.
33401230	5	24	from	HS	1041:1042	arg1	studies					1009:1015	further structural and functional studies	975:1015	further structural and functional studies of O-sulfated groups in HS	975:1042	This provides a foundation for further structural and functional studies of O-sulfated groups in HS.
33401230	3	25	theme	dp4	628:630	arg1	modification					588:599	the chemical modification	575:599	the chemical modification of the fully sulfated dp2, dp4 and dp6	575:638	In this study, a heparin oligosaccharide library, including dp2, dp4 and dp6, were prepared from the chemical modification of the fully sulfated dp2, dp4 and dp6.
33401230	0	26	theme	partial	36:42	arg1	de-O-sulfation					44:57	partial de-O-sulfation	36:57	partial de-O-sulfation of heparin oligosaccharide library	36:92	Preparation and characterization of partial de-O-sulfation of heparin oligosaccharide library.
33401230	2	27	theme	2-O-	361:364	arg1	oligosaccharides					384:399	the naturally-occurring, 2-O- and 6-O-sulfating oligosaccharides	336:399	the naturally-occurring, 2-O- and 6-O-sulfating oligosaccharides from HS	336:407	Therefore, the ability to chemically generate a series of oligosaccharides, which have a similar structure to the naturally-occurring, 2-O- and 6-O-sulfating oligosaccharides from HS, would greatly contribute to investigating their natural role in HS.
33401230	1	28	contain	have	170:173	arg2	roles					219:223	important biological and pathophysiological roles	175:223	important biological and pathophysiological roles	175:223	The O-sulfation, including 2-O- and 6-O-sulfation, in heparan sulfate (HS) have important biological and pathophysiological roles.
33401230	1	28	contain	have	170:173	arg1	O-sulfation					99:109	The O-sulfation	95:109	The O-sulfation	95:109	The O-sulfation, including 2-O- and 6-O-sulfation, in heparan sulfate (HS) have important biological and pathophysiological roles.
33401230	3	29	theme	chemical	579:586	arg1	modification					588:599	the chemical modification	575:599	the chemical modification of the fully sulfated dp2, dp4 and dp6	575:638	In this study, a heparin oligosaccharide library, including dp2, dp4 and dp6, were prepared from the chemical modification of the fully sulfated dp2, dp4 and dp6.
33401230	0	30	theme	heparin	62:68	arg1	library					86:92	heparin oligosaccharide library	62:92	heparin oligosaccharide library	62:92	Preparation and characterization of partial de-O-sulfation of heparin oligosaccharide library.
33401230	2	31	contain	have	308:311	arg1	oligosaccharides					284:299	oligosaccharides	284:299	oligosaccharides	284:299	Therefore, the ability to chemically generate a series of oligosaccharides, which have a similar structure to the naturally-occurring, 2-O- and 6-O-sulfating oligosaccharides from HS, would greatly contribute to investigating their natural role in HS.
33401230	2	31	contain	have	308:311	arg2	structure					323:331	a similar structure	313:331	a similar structure	313:331	Therefore, the ability to chemically generate a series of oligosaccharides, which have a similar structure to the naturally-occurring, 2-O- and 6-O-sulfating oligosaccharides from HS, would greatly contribute to investigating their natural role in HS.
33401230	3	32	theme	dp2	623:625	arg1	modification					588:599	the chemical modification	575:599	the chemical modification of the fully sulfated dp2, dp4 and dp6	575:638	In this study, a heparin oligosaccharide library, including dp2, dp4 and dp6, were prepared from the chemical modification of the fully sulfated dp2, dp4 and dp6.
33401230	5	33	theme	functional	998:1007	arg1	studies					1009:1015	further structural and functional studies	975:1015	further structural and functional studies of O-sulfated groups in HS	975:1042	This provides a foundation for further structural and functional studies of O-sulfated groups in HS.
33401230	2	34	theme	naturally-occurring	340:358	arg1	oligosaccharides					384:399	the naturally-occurring, 2-O- and 6-O-sulfating oligosaccharides	336:399	the naturally-occurring, 2-O- and 6-O-sulfating oligosaccharides from HS	336:407	Therefore, the ability to chemically generate a series of oligosaccharides, which have a similar structure to the naturally-occurring, 2-O- and 6-O-sulfating oligosaccharides from HS, would greatly contribute to investigating their natural role in HS.
33401230	4	35	theme	library	814:820	arg1	structure					797:805	structure	797:805	structure	797:805	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	4	35	theme	library	814:820	arg1	composition					781:791	disaccharide composition	768:791	disaccharide composition	768:791	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	3	36	theme	sulfated	614:621	arg1	dp2					623:625	dp2	623:625	dp2	623:625	In this study, a heparin oligosaccharide library, including dp2, dp4 and dp6, were prepared from the chemical modification of the fully sulfated dp2, dp4 and dp6.
33401230	1	37	theme	heparan	149:155	arg1	sulfate					157:163	heparan sulfate	149:163	heparan sulfate (HS)	149:168	The O-sulfation, including 2-O- and 6-O-sulfation, in heparan sulfate (HS) have important biological and pathophysiological roles.
33401230	1	37	theme	heparan	149:155	arg1	HS					166:167	HS	166:167	HS	166:167	The O-sulfation, including 2-O- and 6-O-sulfation, in heparan sulfate (HS) have important biological and pathophysiological roles.
33401230	5	38	theme	O-sulfated	1020:1029	arg1	groups					1031:1036	O-sulfated groups	1020:1036	O-sulfated groups in HS	1020:1042	This provides a foundation for further structural and functional studies of O-sulfated groups in HS.
33401230	2	39	from	HS	406:407	arg1	oligosaccharides					384:399	the naturally-occurring, 2-O- and 6-O-sulfating oligosaccharides	336:399	the naturally-occurring, 2-O- and 6-O-sulfating oligosaccharides from HS	336:407	Therefore, the ability to chemically generate a series of oligosaccharides, which have a similar structure to the naturally-occurring, 2-O- and 6-O-sulfating oligosaccharides from HS, would greatly contribute to investigating their natural role in HS.
33401230	5	40	theme	further	975:981	arg1	studies					1009:1015	further structural and functional studies	975:1015	further structural and functional studies of O-sulfated groups in HS	975:1042	This provides a foundation for further structural and functional studies of O-sulfated groups in HS.
33401230	4	41	theme	disaccharide	768:779	arg1	composition					781:791	disaccharide composition	768:791	disaccharide composition	768:791	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	4	42	theme	different	697:705	arg1	patterns					707:714	different patterns	697:714	different patterns of 2-O- and 6-O-sulfated oligosaccharides	697:756	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	1	43	from	O-sulfation	99:109	arg1	sulfate					157:163	heparan sulfate	149:163	heparan sulfate (HS)	149:168	The O-sulfation, including 2-O- and 6-O-sulfation, in heparan sulfate (HS) have important biological and pathophysiological roles.
33401230	1	43	from	O-sulfation	99:109	arg1	HS					166:167	HS	166:167	HS	166:167	The O-sulfation, including 2-O- and 6-O-sulfation, in heparan sulfate (HS) have important biological and pathophysiological roles.
33401230	0	44	theme	library	86:92	arg1	de-O-sulfation					44:57	partial de-O-sulfation	36:57	partial de-O-sulfation of heparin oligosaccharide library	36:92	Preparation and characterization of partial de-O-sulfation of heparin oligosaccharide library.
33401230	3	45	theme	dp6	636:638	arg1	modification					588:599	the chemical modification	575:599	the chemical modification of the fully sulfated dp2, dp4 and dp6	575:638	In this study, a heparin oligosaccharide library, including dp2, dp4 and dp6, were prepared from the chemical modification of the fully sulfated dp2, dp4 and dp6.
33401230	2	46	theme	6-O-sulfating	370:382	arg1	oligosaccharides					384:399	the naturally-occurring, 2-O- and 6-O-sulfating oligosaccharides	336:399	the naturally-occurring, 2-O- and 6-O-sulfating oligosaccharides from HS	336:407	Therefore, the ability to chemically generate a series of oligosaccharides, which have a similar structure to the naturally-occurring, 2-O- and 6-O-sulfating oligosaccharides from HS, would greatly contribute to investigating their natural role in HS.
33401230	0	47	theme	oligosaccharide	70:84	arg1	library					86:92	heparin oligosaccharide library	62:92	heparin oligosaccharide library	62:92	Preparation and characterization of partial de-O-sulfation of heparin oligosaccharide library.
33401230	4	48	theme	reaction	650:657	arg1	conditions					659:668	Chemical reaction conditions	641:668	Chemical reaction conditions	641:668	Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis.
33401230	5	49	from	studies	1009:1015	arg1	HS					1041:1042	HS	1041:1042	HS	1041:1042	This provides a foundation for further structural and functional studies of O-sulfated groups in HS.
34688676	4	0	from	process	613:619	arg1	solution					635:642	a 3% CaCl2 solution	624:642	a 3% CaCl2 solution	624:642	Then, polypyrrole nanotubes were added to the sodium alginate gel and the composite beads were prepared by a crosslinking process in a 3% CaCl2 solution.
34688676	12	1	theme	SA/PPyNT	1821:1828	arg1	composites					1830:1839	the SA/PPyNT composites	1817:1839	the SA/PPyNT composites	1817:1839	From the data obtained, it was concluded that the SA/PPyNT composites are promising material as an adsorbent.
34688676	12	1	theme	SA/PPyNT	1821:1828	arg1	material					1855:1862	promising material	1845:1862	promising material	1845:1862	From the data obtained, it was concluded that the SA/PPyNT composites are promising material as an adsorbent.
34688676	2	2	theme	blue	353:356	arg1	dye					358:360	methylene blue dye	343:360	methylene blue dye	343:360	This study the performance of polypyrrole nanotube incorporated sodium alginate (SA/PPyNT) on the adsorptive removal of methylene blue dye from an aqueous solution was investigated.
34688676	10	3	theme	pseudo-second-order	1555:1573	arg1	model					1583:1587	the pseudo-second-order kinetic model	1551:1587	the pseudo-second-order kinetic model (r2 = 0.9976)	1551:1601	As a result of the kinetic studies, it was seen that adsorption followed the pseudo-second-order kinetic model (r2 = 0.9976).
34688676	10	3	theme	pseudo-second-order	1555:1573	arg1	r2 = 0.9976					1590:1600	r2 = 0.9976	1590:1600	r2 = 0.9976	1590:1600	As a result of the kinetic studies, it was seen that adsorption followed the pseudo-second-order kinetic model (r2 = 0.9976).
34688676	9	4	from	666.7 mg/g	1447:1456	arg1	maximum					1436:1442	the maximum	1432:1442	the maximum at 666.7 mg/g	1432:1456	The adsorption capacity showed the maximum at 666.7 mg/g in pH = 7 at 25 °C.
34688676	2	5	theme	methylene	343:351	arg1	dye					358:360	methylene blue dye	343:360	methylene blue dye	343:360	This study the performance of polypyrrole nanotube incorporated sodium alginate (SA/PPyNT) on the adsorptive removal of methylene blue dye from an aqueous solution was investigated.
34688676	12	6	theme	promising	1845:1853	arg1	composites					1830:1839	the SA/PPyNT composites	1817:1839	the SA/PPyNT composites	1817:1839	From the data obtained, it was concluded that the SA/PPyNT composites are promising material as an adsorbent.
34688676	12	6	theme	promising	1845:1853	arg1	material					1855:1862	promising material	1845:1862	promising material	1845:1862	From the data obtained, it was concluded that the SA/PPyNT composites are promising material as an adsorbent.
34688676	5	7	dep	using	684:688	arg1	analyzes					822:829	analyzes	822:829	using Fourier transform infrared-attenuated total reflectance (FTIR-ATR), scanning electron microscope (SEM), and atomic force microscopy analyzes	684:829	The composite beads were characterized using Fourier transform infrared-attenuated total reflectance (FTIR-ATR), scanning electron microscope (SEM), and atomic force microscopy analyzes.
34688676	0	8	from	Adsorption	0:9	arg1	sodium					36:41	sodium	36:41	sodium	36:41	Adsorption of methylene blue dye on sodium alginate/polypyrrole nanotube composites.
34688676	5	9	theme	composite	649:657	arg1	beads					659:663	The composite beads	645:663	The composite beads	645:663	The composite beads were characterized using Fourier transform infrared-attenuated total reflectance (FTIR-ATR), scanning electron microscope (SEM), and atomic force microscopy analyzes.
34688676	6	10	theme	contact	980:986	arg1	time					988:991	contact time	980:991	contact time	980:991	In the adsorption studies, to determine the optimum conditions, experiments were carried out at different conditions namely temperature (25-45 °C), contact time, initial pH (2-12), adsorbent dosage (1-5 g/L), dye concentrations (10-50 mg/L).
34688676	6	10	theme	contact	980:986	arg1	temperature					956:966	temperature	956:966	temperature (25-45 °C)	956:977	In the adsorption studies, to determine the optimum conditions, experiments were carried out at different conditions namely temperature (25-45 °C), contact time, initial pH (2-12), adsorbent dosage (1-5 g/L), dye concentrations (10-50 mg/L).
34688676	5	11	dep	Fourier	690:696	arg1	transform					698:706	transform	698:706	transform infrared-attenuated total reflectance (FTIR-ATR), scanning electron microscope (SEM), and atomic force microscopy	698:820	The composite beads were characterized using Fourier transform infrared-attenuated total reflectance (FTIR-ATR), scanning electron microscope (SEM), and atomic force microscopy analyzes.
34688676	11	12	dep	and	1727:1729	arg1	kJ/mol					1719:1724	∆HA = - 68.1 kJ/mol	1706:1724	∆HA = - 68.1 kJ/mol	1706:1724	When thermodynamic parameters were examined, it was seen that the adsorption occurred exothermically (∆HA = - 68.1 kJ/mol) and spontaneously (∆GA298 = - 27.4 kJ/mol).
34688676	11	12	dep	and	1727:1729	arg1	kJ/mol					1762:1767	∆GA298 = - 27.4 kJ/mol	1746:1767	∆GA298 = - 27.4 kJ/mol	1746:1767	When thermodynamic parameters were examined, it was seen that the adsorption occurred exothermically (∆HA = - 68.1 kJ/mol) and spontaneously (∆GA298 = - 27.4 kJ/mol).
34688676	2	13	theme	sodium	287:292	arg1	SA/PPyNT					304:311	SA/PPyNT	304:311	SA/PPyNT	304:311	This study the performance of polypyrrole nanotube incorporated sodium alginate (SA/PPyNT) on the adsorptive removal of methylene blue dye from an aqueous solution was investigated.
34688676	2	13	theme	sodium	287:292	arg1	alginate					294:301	sodium alginate	287:301	sodium alginate (SA/PPyNT)	287:312	This study the performance of polypyrrole nanotube incorporated sodium alginate (SA/PPyNT) on the adsorptive removal of methylene blue dye from an aqueous solution was investigated.
34688676	6	14	theme	adsorbent	1013:1021	arg1	1-5 g/L					1031:1037	1-5 g/L	1031:1037	1-5 g/L	1031:1037	In the adsorption studies, to determine the optimum conditions, experiments were carried out at different conditions namely temperature (25-45 °C), contact time, initial pH (2-12), adsorbent dosage (1-5 g/L), dye concentrations (10-50 mg/L).
34688676	6	14	theme	adsorbent	1013:1021	arg1	dosage					1023:1028	adsorbent dosage	1013:1028	adsorbent dosage (1-5 g/L)	1013:1038	In the adsorption studies, to determine the optimum conditions, experiments were carried out at different conditions namely temperature (25-45 °C), contact time, initial pH (2-12), adsorbent dosage (1-5 g/L), dye concentrations (10-50 mg/L).
34688676	6	14	theme	adsorbent	1013:1021	arg1	temperature					956:966	temperature	956:966	temperature (25-45 °C)	956:977	In the adsorption studies, to determine the optimum conditions, experiments were carried out at different conditions namely temperature (25-45 °C), contact time, initial pH (2-12), adsorbent dosage (1-5 g/L), dye concentrations (10-50 mg/L).
34688676	2	15	theme	aqueous	370:376	arg1	solution					378:385	an aqueous solution	367:385	an aqueous solution	367:385	This study the performance of polypyrrole nanotube incorporated sodium alginate (SA/PPyNT) on the adsorptive removal of methylene blue dye from an aqueous solution was investigated.
34688676	1	16	theme	high	167:170	arg1	surface					172:178	their high surface	161:178	their high surface	161:178	Nanomaterials have recently come to the fore as potential adsorbents due to their high surface, high efficiency, and adsorption capacity.
34688676	4	17	theme	polypyrrole	497:507	arg1	nanotubes					509:517	polypyrrole nanotubes	497:517	polypyrrole nanotubes	497:517	Then, polypyrrole nanotubes were added to the sodium alginate gel and the composite beads were prepared by a crosslinking process in a 3% CaCl2 solution.
34688676	2	18	theme	nanotube	265:272	arg1	performance					238:248	the performance	234:248	the performance of polypyrrole nanotube	234:272	This study the performance of polypyrrole nanotube incorporated sodium alginate (SA/PPyNT) on the adsorptive removal of methylene blue dye from an aqueous solution was investigated.
34688676	5	19	theme	atomic	798:803	arg1	microscopy					811:820	atomic force microscopy	798:820	atomic force microscopy	798:820	The composite beads were characterized using Fourier transform infrared-attenuated total reflectance (FTIR-ATR), scanning electron microscope (SEM), and atomic force microscopy analyzes.
34688676	10	20	theme	studies	1505:1511	arg1	result					1483:1488	a result	1481:1488	a result of the kinetic studies	1481:1511	As a result of the kinetic studies, it was seen that adsorption followed the pseudo-second-order kinetic model (r2 = 0.9976).
34688676	10	20	theme	studies	1505:1511	arg1	it					1514:1515	it	1514:1515	it	1514:1515	As a result of the kinetic studies, it was seen that adsorption followed the pseudo-second-order kinetic model (r2 = 0.9976).
34688676	7	21	theme	isotherm	1194:1201	arg1	D-R					1240:1242	D-R	1240:1242	D-R	1240:1242	The studies indicated that the removal percentage of MB reached up to 90.5% at pH = 7 and 25 °C. Furthermore, different isotherm models such as Freundlich, Langmuir, D-R, and Harkins-Jura were applied.
34688676	7	21	theme	isotherm	1194:1201	arg1	Harkins-Jura					1249:1260	Harkins-Jura	1249:1260	Harkins-Jura	1249:1260	The studies indicated that the removal percentage of MB reached up to 90.5% at pH = 7 and 25 °C. Furthermore, different isotherm models such as Freundlich, Langmuir, D-R, and Harkins-Jura were applied.
34688676	7	21	theme	isotherm	1194:1201	arg1	Langmuir					1230:1237	Langmuir	1230:1237	Langmuir	1230:1237	The studies indicated that the removal percentage of MB reached up to 90.5% at pH = 7 and 25 °C. Furthermore, different isotherm models such as Freundlich, Langmuir, D-R, and Harkins-Jura were applied.
34688676	7	21	theme	isotherm	1194:1201	arg1	models					1203:1208	different isotherm models	1184:1208	different isotherm models such as Freundlich, Langmuir, D-R, and Harkins-Jura	1184:1260	The studies indicated that the removal percentage of MB reached up to 90.5% at pH = 7 and 25 °C. Furthermore, different isotherm models such as Freundlich, Langmuir, D-R, and Harkins-Jura were applied.
34688676	7	21	theme	isotherm	1194:1201	arg1	Freundlich					1218:1227	Freundlich	1218:1227	Freundlich	1218:1227	The studies indicated that the removal percentage of MB reached up to 90.5% at pH = 7 and 25 °C. Furthermore, different isotherm models such as Freundlich, Langmuir, D-R, and Harkins-Jura were applied.
34688676	7	22	theme	MB	1127:1128	arg1	percentage					1113:1122	the removal percentage	1101:1122	the removal percentage of MB	1101:1128	The studies indicated that the removal percentage of MB reached up to 90.5% at pH = 7 and 25 °C. Furthermore, different isotherm models such as Freundlich, Langmuir, D-R, and Harkins-Jura were applied.
34688676	3	23	theme	polypyrrole	414:424	arg1	nanotubes					426:434	polypyrrole nanotubes	414:434	polypyrrole nanotubes	414:434	Firstly, polypyrrole nanotubes were synthesized by oxidative chemical polymerization.
34688676	5	24	theme	force	805:809	arg1	microscopy					811:820	atomic force microscopy	798:820	atomic force microscopy	798:820	The composite beads were characterized using Fourier transform infrared-attenuated total reflectance (FTIR-ATR), scanning electron microscope (SEM), and atomic force microscopy analyzes.
34688676	8	25	theme	correlation	1292:1302	arg1	coefficients					1304:1315	the correlation coefficients	1288:1315	the correlation coefficients	1288:1315	Considering the correlation coefficients, the Langmuir isotherm model was found to be the most suitable model (r2 = 0.9974).
34688676	1	26	theme	high	181:184	arg1	efficiency					186:195	high efficiency	181:195	high efficiency	181:195	Nanomaterials have recently come to the fore as potential adsorbents due to their high surface, high efficiency, and adsorption capacity.
34688676	0	27	theme	blue	24:27	arg1	dye					29:31	methylene blue dye	14:31	methylene blue dye	14:31	Adsorption of methylene blue dye on sodium alginate/polypyrrole nanotube composites.
34688676	10	28	theme	kinetic	1497:1503	arg1	studies					1505:1511	the kinetic studies	1493:1511	the kinetic studies	1493:1511	As a result of the kinetic studies, it was seen that adsorption followed the pseudo-second-order kinetic model (r2 = 0.9976).
34688676	8	29	theme	isotherm	1331:1338	arg1	model					1340:1344	the Langmuir isotherm model	1318:1344	the Langmuir isotherm model	1318:1344	Considering the correlation coefficients, the Langmuir isotherm model was found to be the most suitable model (r2 = 0.9974).
34688676	8	29	theme	isotherm	1331:1338	arg1	model					1380:1384	the most suitable model	1362:1384	the most suitable model (r2 = 0.9974)	1362:1398	Considering the correlation coefficients, the Langmuir isotherm model was found to be the most suitable model (r2 = 0.9974).
34688676	3	30	theme	chemical	466:473	arg1	polymerization					475:488	oxidative chemical polymerization	456:488	oxidative chemical polymerization	456:488	Firstly, polypyrrole nanotubes were synthesized by oxidative chemical polymerization.
34688676	0	31	theme	methylene	14:22	arg1	dye					29:31	methylene blue dye	14:31	methylene blue dye	14:31	Adsorption of methylene blue dye on sodium alginate/polypyrrole nanotube composites.
34688676	5	32	theme	infrared-attenuated	708:726	arg1	FTIR-ATR					747:754	FTIR-ATR	747:754	FTIR-ATR	747:754	The composite beads were characterized using Fourier transform infrared-attenuated total reflectance (FTIR-ATR), scanning electron microscope (SEM), and atomic force microscopy analyzes.
34688676	5	32	theme	infrared-attenuated	708:726	arg1	reflectance					734:744	infrared-attenuated total reflectance	708:744	infrared-attenuated total reflectance (FTIR-ATR)	708:755	The composite beads were characterized using Fourier transform infrared-attenuated total reflectance (FTIR-ATR), scanning electron microscope (SEM), and atomic force microscopy analyzes.
34688676	8	33	theme	suitable	1371:1378	arg1	model					1380:1384	the most suitable model	1362:1384	the most suitable model (r2 = 0.9974)	1362:1398	Considering the correlation coefficients, the Langmuir isotherm model was found to be the most suitable model (r2 = 0.9974).
34688676	8	33	theme	suitable	1371:1378	arg1	model					1340:1344	the Langmuir isotherm model	1318:1344	the Langmuir isotherm model	1318:1344	Considering the correlation coefficients, the Langmuir isotherm model was found to be the most suitable model (r2 = 0.9974).
34688676	8	33	theme	suitable	1371:1378	arg1	r2 = 0.9974					1387:1397	r2 = 0.9974	1387:1397	r2 = 0.9974	1387:1397	Considering the correlation coefficients, the Langmuir isotherm model was found to be the most suitable model (r2 = 0.9974).
34688676	6	34	theme	dye	1041:1043	arg1	10-50 mg/L					1061:1070	10-50 mg/L	1061:1070	10-50 mg/L	1061:1070	In the adsorption studies, to determine the optimum conditions, experiments were carried out at different conditions namely temperature (25-45 °C), contact time, initial pH (2-12), adsorbent dosage (1-5 g/L), dye concentrations (10-50 mg/L).
34688676	6	34	theme	dye	1041:1043	arg1	concentrations					1045:1058	dye concentrations	1041:1058	dye concentrations (10-50 mg/L)	1041:1071	In the adsorption studies, to determine the optimum conditions, experiments were carried out at different conditions namely temperature (25-45 °C), contact time, initial pH (2-12), adsorbent dosage (1-5 g/L), dye concentrations (10-50 mg/L).
34688676	6	34	theme	dye	1041:1043	arg1	temperature					956:966	temperature	956:966	temperature (25-45 °C)	956:977	In the adsorption studies, to determine the optimum conditions, experiments were carried out at different conditions namely temperature (25-45 °C), contact time, initial pH (2-12), adsorbent dosage (1-5 g/L), dye concentrations (10-50 mg/L).
34688676	4	35	theme	3	626:626	arg1	%					627:627	%	627:627	%	627:627	Then, polypyrrole nanotubes were added to the sodium alginate gel and the composite beads were prepared by a crosslinking process in a 3% CaCl2 solution.
34688676	4	36	theme	composite	565:573	arg1	beads					575:579	the composite beads	561:579	the composite beads	561:579	Then, polypyrrole nanotubes were added to the sodium alginate gel and the composite beads were prepared by a crosslinking process in a 3% CaCl2 solution.
34688676	5	37	theme	total	728:732	arg1	FTIR-ATR					747:754	FTIR-ATR	747:754	FTIR-ATR	747:754	The composite beads were characterized using Fourier transform infrared-attenuated total reflectance (FTIR-ATR), scanning electron microscope (SEM), and atomic force microscopy analyzes.
34688676	5	37	theme	total	728:732	arg1	reflectance					734:744	infrared-attenuated total reflectance	708:744	infrared-attenuated total reflectance (FTIR-ATR)	708:755	The composite beads were characterized using Fourier transform infrared-attenuated total reflectance (FTIR-ATR), scanning electron microscope (SEM), and atomic force microscopy analyzes.
34688676	11	38	theme	thermodynamic	1609:1621	arg1	parameters					1623:1632	thermodynamic parameters	1609:1632	thermodynamic parameters	1609:1632	When thermodynamic parameters were examined, it was seen that the adsorption occurred exothermically (∆HA = - 68.1 kJ/mol) and spontaneously (∆GA298 = - 27.4 kJ/mol).
34688676	4	39	theme	CaCl2	629:633	arg1	solution					635:642	a 3% CaCl2 solution	624:642	a 3% CaCl2 solution	624:642	Then, polypyrrole nanotubes were added to the sodium alginate gel and the composite beads were prepared by a crosslinking process in a 3% CaCl2 solution.
34688676	2	40	dep	study	228:232	arg1	incorporated					274:285	incorporated	274:285	incorporated sodium alginate (SA/PPyNT) on the adsorptive removal of methylene blue dye from an aqueous solution	274:385	This study the performance of polypyrrole nanotube incorporated sodium alginate (SA/PPyNT) on the adsorptive removal of methylene blue dye from an aqueous solution was investigated.
34688676	0	41	theme	dye	29:31	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption of methylene blue dye on sodium	0:41	Adsorption of methylene blue dye on sodium alginate/polypyrrole nanotube composites.
34688676	4	42	theme	sodium	537:542	arg1	gel					553:555	the sodium alginate gel	533:555	the sodium alginate gel	533:555	Then, polypyrrole nanotubes were added to the sodium alginate gel and the composite beads were prepared by a crosslinking process in a 3% CaCl2 solution.
34688676	7	43	theme	removal	1105:1111	arg1	percentage					1113:1122	the removal percentage	1101:1122	the removal percentage of MB	1101:1128	The studies indicated that the removal percentage of MB reached up to 90.5% at pH = 7 and 25 °C. Furthermore, different isotherm models such as Freundlich, Langmuir, D-R, and Harkins-Jura were applied.
34688676	8	44	theme	Langmuir	1322:1329	arg1	model					1340:1344	the Langmuir isotherm model	1318:1344	the Langmuir isotherm model	1318:1344	Considering the correlation coefficients, the Langmuir isotherm model was found to be the most suitable model (r2 = 0.9974).
34688676	8	44	theme	Langmuir	1322:1329	arg1	model					1380:1384	the most suitable model	1362:1384	the most suitable model (r2 = 0.9974)	1362:1398	Considering the correlation coefficients, the Langmuir isotherm model was found to be the most suitable model (r2 = 0.9974).
34688676	3	45	theme	oxidative	456:464	arg1	polymerization					475:488	oxidative chemical polymerization	456:488	oxidative chemical polymerization	456:488	Firstly, polypyrrole nanotubes were synthesized by oxidative chemical polymerization.
34688676	5	46	theme	electron	767:774	arg1	SEM					788:790	SEM	788:790	SEM	788:790	The composite beads were characterized using Fourier transform infrared-attenuated total reflectance (FTIR-ATR), scanning electron microscope (SEM), and atomic force microscopy analyzes.
34688676	5	46	theme	electron	767:774	arg1	microscope					776:785	scanning electron microscope	758:785	scanning electron microscope (SEM)	758:791	The composite beads were characterized using Fourier transform infrared-attenuated total reflectance (FTIR-ATR), scanning electron microscope (SEM), and atomic force microscopy analyzes.
34688676	6	47	theme	optimum	876:882	arg1	conditions					884:893	the optimum conditions	872:893	the optimum conditions	872:893	In the adsorption studies, to determine the optimum conditions, experiments were carried out at different conditions namely temperature (25-45 °C), contact time, initial pH (2-12), adsorbent dosage (1-5 g/L), dye concentrations (10-50 mg/L).
34688676	9	48	theme	adsorption	1405:1414	arg1	capacity					1416:1423	The adsorption capacity	1401:1423	The adsorption capacity	1401:1423	The adsorption capacity showed the maximum at 666.7 mg/g in pH = 7 at 25 °C.
34688676	7	49	dep	25 °C.	1164:1169	arg1	applied					1267:1273	applied	1267:1273	were applied	1262:1273	The studies indicated that the removal percentage of MB reached up to 90.5% at pH = 7 and 25 °C. Furthermore, different isotherm models such as Freundlich, Langmuir, D-R, and Harkins-Jura were applied.
34688676	6	50	theme	different	928:936	arg1	conditions					938:947	different conditions	928:947	different conditions	928:947	In the adsorption studies, to determine the optimum conditions, experiments were carried out at different conditions namely temperature (25-45 °C), contact time, initial pH (2-12), adsorbent dosage (1-5 g/L), dye concentrations (10-50 mg/L).
34688676	6	51	theme	adsorption	839:848	arg1	studies					850:856	the adsorption studies	835:856	the adsorption studies	835:856	In the adsorption studies, to determine the optimum conditions, experiments were carried out at different conditions namely temperature (25-45 °C), contact time, initial pH (2-12), adsorbent dosage (1-5 g/L), dye concentrations (10-50 mg/L).
34688676	1	52	theme	due	154:156	arg1	adsorbents					143:152	potential adsorbents	133:152	potential adsorbents due to their high surface, high efficiency, and adsorption capacity	133:220	Nanomaterials have recently come to the fore as potential adsorbents due to their high surface, high efficiency, and adsorption capacity.
34688676	4	53	theme	alginate	544:551	arg1	gel					553:555	the sodium alginate gel	533:555	the sodium alginate gel	533:555	Then, polypyrrole nanotubes were added to the sodium alginate gel and the composite beads were prepared by a crosslinking process in a 3% CaCl2 solution.
34688676	7	54	dep	90.5	1144:1147	arg1	to					1141:1142	to	1141:1142	to	1141:1142	The studies indicated that the removal percentage of MB reached up to 90.5% at pH = 7 and 25 °C. Furthermore, different isotherm models such as Freundlich, Langmuir, D-R, and Harkins-Jura were applied.
34688676	2	55	theme	polypyrrole	253:263	arg1	nanotube					265:272	polypyrrole nanotube	253:272	polypyrrole nanotube	253:272	This study the performance of polypyrrole nanotube incorporated sodium alginate (SA/PPyNT) on the adsorptive removal of methylene blue dye from an aqueous solution was investigated.
34688676	2	56	from	solution	378:385	arg1	removal					332:338	the adsorptive removal	317:338	the adsorptive removal of methylene blue dye from an aqueous solution	317:385	This study the performance of polypyrrole nanotube incorporated sodium alginate (SA/PPyNT) on the adsorptive removal of methylene blue dye from an aqueous solution was investigated.
34688676	2	57	theme	adsorptive	321:330	arg1	removal					332:338	the adsorptive removal	317:338	the adsorptive removal of methylene blue dye from an aqueous solution	317:385	This study the performance of polypyrrole nanotube incorporated sodium alginate (SA/PPyNT) on the adsorptive removal of methylene blue dye from an aqueous solution was investigated.
34688676	10	58	theme	kinetic	1575:1581	arg1	model					1583:1587	the pseudo-second-order kinetic model	1551:1587	the pseudo-second-order kinetic model (r2 = 0.9976)	1551:1601	As a result of the kinetic studies, it was seen that adsorption followed the pseudo-second-order kinetic model (r2 = 0.9976).
34688676	10	58	theme	kinetic	1575:1581	arg1	r2 = 0.9976					1590:1600	r2 = 0.9976	1590:1600	r2 = 0.9976	1590:1600	As a result of the kinetic studies, it was seen that adsorption followed the pseudo-second-order kinetic model (r2 = 0.9976).
34688676	1	59	theme	potential	133:141	arg1	adsorbents					143:152	potential adsorbents	133:152	potential adsorbents due to their high surface, high efficiency, and adsorption capacity	133:220	Nanomaterials have recently come to the fore as potential adsorbents due to their high surface, high efficiency, and adsorption capacity.
34688676	4	60	theme	crosslinking	600:611	arg1	process					613:619	a crosslinking process	598:619	a crosslinking process in a 3% CaCl2 solution	598:642	Then, polypyrrole nanotubes were added to the sodium alginate gel and the composite beads were prepared by a crosslinking process in a 3% CaCl2 solution.
34688676	5	61	theme	scanning	758:765	arg1	SEM					788:790	SEM	788:790	SEM	788:790	The composite beads were characterized using Fourier transform infrared-attenuated total reflectance (FTIR-ATR), scanning electron microscope (SEM), and atomic force microscopy analyzes.
34688676	5	61	theme	scanning	758:765	arg1	microscope					776:785	scanning electron microscope	758:785	scanning electron microscope (SEM)	758:791	The composite beads were characterized using Fourier transform infrared-attenuated total reflectance (FTIR-ATR), scanning electron microscope (SEM), and atomic force microscopy analyzes.
34688676	1	62	theme	adsorption	202:211	arg1	capacity					213:220	adsorption capacity	202:220	adsorption capacity	202:220	Nanomaterials have recently come to the fore as potential adsorbents due to their high surface, high efficiency, and adsorption capacity.
34688676	4	63	theme	%	627:627	arg1	solution					635:642	a 3% CaCl2 solution	624:642	a 3% CaCl2 solution	624:642	Then, polypyrrole nanotubes were added to the sodium alginate gel and the composite beads were prepared by a crosslinking process in a 3% CaCl2 solution.
34688676	7	64	theme	different	1184:1192	arg1	D-R					1240:1242	D-R	1240:1242	D-R	1240:1242	The studies indicated that the removal percentage of MB reached up to 90.5% at pH = 7 and 25 °C. Furthermore, different isotherm models such as Freundlich, Langmuir, D-R, and Harkins-Jura were applied.
34688676	7	64	theme	different	1184:1192	arg1	Harkins-Jura					1249:1260	Harkins-Jura	1249:1260	Harkins-Jura	1249:1260	The studies indicated that the removal percentage of MB reached up to 90.5% at pH = 7 and 25 °C. Furthermore, different isotherm models such as Freundlich, Langmuir, D-R, and Harkins-Jura were applied.
34688676	7	64	theme	different	1184:1192	arg1	Langmuir					1230:1237	Langmuir	1230:1237	Langmuir	1230:1237	The studies indicated that the removal percentage of MB reached up to 90.5% at pH = 7 and 25 °C. Furthermore, different isotherm models such as Freundlich, Langmuir, D-R, and Harkins-Jura were applied.
34688676	7	64	theme	different	1184:1192	arg1	models					1203:1208	different isotherm models	1184:1208	different isotherm models such as Freundlich, Langmuir, D-R, and Harkins-Jura	1184:1260	The studies indicated that the removal percentage of MB reached up to 90.5% at pH = 7 and 25 °C. Furthermore, different isotherm models such as Freundlich, Langmuir, D-R, and Harkins-Jura were applied.
34688676	7	64	theme	different	1184:1192	arg1	Freundlich					1218:1227	Freundlich	1218:1227	Freundlich	1218:1227	The studies indicated that the removal percentage of MB reached up to 90.5% at pH = 7 and 25 °C. Furthermore, different isotherm models such as Freundlich, Langmuir, D-R, and Harkins-Jura were applied.
34688676	0	65	theme	nanotube	64:71	arg1	composites					73:82	nanotube composites	64:82	nanotube composites	64:82	Adsorption of methylene blue dye on sodium alginate/polypyrrole nanotube composites.
34688676	2	66	theme	dye	358:360	arg1	removal					332:338	the adsorptive removal	317:338	the adsorptive removal of methylene blue dye from an aqueous solution	317:385	This study the performance of polypyrrole nanotube incorporated sodium alginate (SA/PPyNT) on the adsorptive removal of methylene blue dye from an aqueous solution was investigated.
34688676	6	67	theme	initial	994:1000	arg1	pH					1002:1003	initial pH	994:1003	initial pH (2-12)	994:1010	In the adsorption studies, to determine the optimum conditions, experiments were carried out at different conditions namely temperature (25-45 °C), contact time, initial pH (2-12), adsorbent dosage (1-5 g/L), dye concentrations (10-50 mg/L).
34688676	6	67	theme	initial	994:1000	arg1	temperature					956:966	temperature	956:966	temperature (25-45 °C)	956:977	In the adsorption studies, to determine the optimum conditions, experiments were carried out at different conditions namely temperature (25-45 °C), contact time, initial pH (2-12), adsorbent dosage (1-5 g/L), dye concentrations (10-50 mg/L).
34688676	6	67	theme	initial	994:1000	arg1	2-12					1006:1009	2-12	1006:1009	2-12	1006:1009	In the adsorption studies, to determine the optimum conditions, experiments were carried out at different conditions namely temperature (25-45 °C), contact time, initial pH (2-12), adsorbent dosage (1-5 g/L), dye concentrations (10-50 mg/L).
32905737	4	0	theme	analysis	745:752	arg1	results					754:760	thermogravimetric (TG) analysis results	722:760	thermogravimetric (TG) analysis results	722:760	X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, and thermogravimetric (TG) analysis results showed some interactions among the molecules of BC, gelatin, and chitosan within the film.
32905737	7	1	theme	"	1177:1177	arg1	films					1205:1209	The "all-natural" ternary polymer composite films	1161:1209	The "all-natural" ternary polymer composite films of BC-C-G	1161:1219	The "all-natural" ternary polymer composite films of BC-C-G have not been evaluated before for biomedical applications.
32905737	1	2	theme	bacterial	183:191	arg1	BC-C-G					221:226	BC-C-G	221:226	BC-C-G	221:226	Ternary nano-biocomposite films of bacterial cellulose-chitosan-gelatin (BC-C-G) were fabricated by immersing the BC pellicles into chitosan and gelatin mixture and subsequently freeze-drying.
32905737	1	2	theme	bacterial	183:191	arg1	cellulose-chitosan-gelatin					193:218	bacterial cellulose-chitosan-gelatin	183:218	bacterial cellulose-chitosan-gelatin (BC-C-G)	183:227	Ternary nano-biocomposite films of bacterial cellulose-chitosan-gelatin (BC-C-G) were fabricated by immersing the BC pellicles into chitosan and gelatin mixture and subsequently freeze-drying.
32905737	4	3	theme	X-ray	629:633	arg1	diffraction					635:645	X-ray diffraction	629:645	X-ray diffraction	629:645	X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, and thermogravimetric (TG) analysis results showed some interactions among the molecules of BC, gelatin, and chitosan within the film.
32905737	7	4	theme	all-natural	1166:1176	arg1	films					1205:1209	The "all-natural" ternary polymer composite films	1161:1209	The "all-natural" ternary polymer composite films of BC-C-G	1161:1219	The "all-natural" ternary polymer composite films of BC-C-G have not been evaluated before for biomedical applications.
32905737	7	5	theme	ternary	1179:1185	arg1	films					1205:1209	The "all-natural" ternary polymer composite films	1161:1209	The "all-natural" ternary polymer composite films of BC-C-G	1161:1219	The "all-natural" ternary polymer composite films of BC-C-G have not been evaluated before for biomedical applications.
32905737	1	6	theme	cellulose-chitosan-gelatin	193:218	arg1	films					174:178	Ternary nano-biocomposite films	148:178	Ternary nano-biocomposite films of bacterial cellulose-chitosan-gelatin (BC-C-G)	148:227	Ternary nano-biocomposite films of bacterial cellulose-chitosan-gelatin (BC-C-G) were fabricated by immersing the BC pellicles into chitosan and gelatin mixture and subsequently freeze-drying.
32905737	1	7	theme	gelatin	293:299	arg1	mixture					301:307	gelatin mixture	293:307	gelatin mixture	293:307	Ternary nano-biocomposite films of bacterial cellulose-chitosan-gelatin (BC-C-G) were fabricated by immersing the BC pellicles into chitosan and gelatin mixture and subsequently freeze-drying.
32905737	2	8	theme	Scanning	341:348	arg1	microscopy					359:368	Scanning electron microscopy	341:368	Scanning electron microscopy (SEM) images of the nano-biocomposite films	341:412	Scanning electron microscopy (SEM) images of the nano-biocomposite films revealed the presence of interconnected pores, with fibre diameter 20-150 nm.
32905737	2	8	theme	Scanning	341:348	arg1	SEM					371:373	SEM	371:373	SEM	371:373	Scanning electron microscopy (SEM) images of the nano-biocomposite films revealed the presence of interconnected pores, with fibre diameter 20-150 nm.
32905737	2	9	theme	electron	350:357	arg1	microscopy					359:368	Scanning electron microscopy	341:368	Scanning electron microscopy (SEM) images of the nano-biocomposite films	341:412	Scanning electron microscopy (SEM) images of the nano-biocomposite films revealed the presence of interconnected pores, with fibre diameter 20-150 nm.
32905737	2	9	theme	electron	350:357	arg1	SEM					371:373	SEM	371:373	SEM	371:373	Scanning electron microscopy (SEM) images of the nano-biocomposite films revealed the presence of interconnected pores, with fibre diameter 20-150 nm.
32905737	7	10	theme	biomedical	1256:1265	arg1	applications					1267:1278	biomedical applications	1256:1278	biomedical applications	1256:1278	The "all-natural" ternary polymer composite films of BC-C-G have not been evaluated before for biomedical applications.
32905737	4	11	theme	gelatin	814:820	arg1	molecules					797:805	the molecules	793:805	the molecules of BC, gelatin, and chitosan	793:834	X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, and thermogravimetric (TG) analysis results showed some interactions among the molecules of BC, gelatin, and chitosan within the film.
32905737	2	12	theme	films	408:412	arg1	images					376:381	Scanning electron microscopy (SEM) images	341:381	Scanning electron microscopy (SEM) images of the nano-biocomposite films	341:412	Scanning electron microscopy (SEM) images of the nano-biocomposite films revealed the presence of interconnected pores, with fibre diameter 20-150 nm.
32905737	5	13	theme	attachment	974:983	arg1	study					985:989	the cell attachment study	965:989	the cell attachment study	965:989	The composite film offered good matrix for adhesion and proliferation of L929 fibroblasts cells as indicated by the cell attachment study, FE-SEM of cell-film constructs and cytocompatibility assay.
32905737	0	14	theme	engineering	122:132	arg1	applications					134:145	tissue engineering applications	115:145	tissue engineering applications	115:145	Ternary nano-biocomposite films using synergistic combination of bacterial cellulose with chitosan and gelatin for tissue engineering applications.
32905737	6	15	theme	tissue	1132:1137	arg1	engineering					1139:1149	tissue engineering	1132:1149	tissue engineering scaffold	1132:1158	Thus, the nano-biocomposite films of BC-C-G could be of paramount importance as tissue engineering scaffold.
32905737	4	16	theme	chitosan	827:834	arg1	molecules					797:805	the molecules	793:805	the molecules of BC, gelatin, and chitosan	793:834	X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, and thermogravimetric (TG) analysis results showed some interactions among the molecules of BC, gelatin, and chitosan within the film.
32905737	5	17	theme	cell	969:972	arg1	study					985:989	the cell attachment study	965:989	the cell attachment study	965:989	The composite film offered good matrix for adhesion and proliferation of L929 fibroblasts cells as indicated by the cell attachment study, FE-SEM of cell-film constructs and cytocompatibility assay.
32905737	2	18	theme	pores	454:458	arg1	presence					427:434	the presence	423:434	the presence of interconnected pores	423:458	Scanning electron microscopy (SEM) images of the nano-biocomposite films revealed the presence of interconnected pores, with fibre diameter 20-150 nm.
32905737	0	19	theme	tissue	115:120	arg1	applications					134:145	tissue engineering applications	115:145	tissue engineering applications	115:145	Ternary nano-biocomposite films using synergistic combination of bacterial cellulose with chitosan and gelatin for tissue engineering applications.
32905737	5	20	theme	cytocompatibility	1027:1043	arg1	assay					1045:1049	cytocompatibility assay	1027:1049	cytocompatibility assay	1027:1049	The composite film offered good matrix for adhesion and proliferation of L929 fibroblasts cells as indicated by the cell attachment study, FE-SEM of cell-film constructs and cytocompatibility assay.
32905737	6	21	theme	engineering	1139:1149	arg1	scaffold					1151:1158	tissue engineering scaffold	1132:1158	tissue engineering scaffold	1132:1158	Thus, the nano-biocomposite films of BC-C-G could be of paramount importance as tissue engineering scaffold.
32905737	2	22	theme	interconnected	439:452	arg1	pores					454:458	interconnected pores	439:458	interconnected pores	439:458	Scanning electron microscopy (SEM) images of the nano-biocomposite films revealed the presence of interconnected pores, with fibre diameter 20-150 nm.
32905737	0	23	theme	nano-biocomposite	8:24	arg1	films					26:30	Ternary nano-biocomposite films	0:30	Ternary nano-biocomposite films	0:30	Ternary nano-biocomposite films using synergistic combination of bacterial cellulose with chitosan and gelatin for tissue engineering applications.
32905737	4	24	theme	TG	741:742	arg1	results					754:760	thermogravimetric (TG) analysis results	722:760	thermogravimetric (TG) analysis results	722:760	X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, and thermogravimetric (TG) analysis results showed some interactions among the molecules of BC, gelatin, and chitosan within the film.
32905737	0	25	theme	Ternary	0:6	arg1	films					26:30	Ternary nano-biocomposite films	0:30	Ternary nano-biocomposite films	0:30	Ternary nano-biocomposite films using synergistic combination of bacterial cellulose with chitosan and gelatin for tissue engineering applications.
32905737	4	26	theme	thermogravimetric	722:738	arg1	results					754:760	thermogravimetric (TG) analysis results	722:760	thermogravimetric (TG) analysis results	722:760	X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, and thermogravimetric (TG) analysis results showed some interactions among the molecules of BC, gelatin, and chitosan within the film.
32905737	3	27	theme	degradability	614:626	arg1	ratio					583:587	ratio	583:587	ratio of 19 ± 1.8 and in vitro degradability	583:626	The composite films have a porosity of 95.3%, and showed good hydrophilicity with swelling ratio of 19 ± 1.8 and in vitro degradability.
32905737	5	28	theme	L929	926:929	arg1	cells					943:947	L929 fibroblasts cells	926:947	L929 fibroblasts cells	926:947	The composite film offered good matrix for adhesion and proliferation of L929 fibroblasts cells as indicated by the cell attachment study, FE-SEM of cell-film constructs and cytocompatibility assay.
32905737	7	29	theme	BC-C-G	1214:1219	arg1	films					1205:1209	The "all-natural" ternary polymer composite films	1161:1209	The "all-natural" ternary polymer composite films of BC-C-G	1161:1219	The "all-natural" ternary polymer composite films of BC-C-G have not been evaluated before for biomedical applications.
32905737	5	30	theme	composite	857:865	arg1	film					867:870	The composite film	853:870	The composite film	853:870	The composite film offered good matrix for adhesion and proliferation of L929 fibroblasts cells as indicated by the cell attachment study, FE-SEM of cell-film constructs and cytocompatibility assay.
32905737	2	31	theme	microscopy	359:368	arg1	images					376:381	Scanning electron microscopy (SEM) images	341:381	Scanning electron microscopy (SEM) images of the nano-biocomposite films	341:412	Scanning electron microscopy (SEM) images of the nano-biocomposite films revealed the presence of interconnected pores, with fibre diameter 20-150 nm.
32905737	5	32	theme	good	880:883	arg1	matrix					885:890	good matrix	880:890	good matrix for adhesion and proliferation of L929 fibroblasts cells	880:947	The composite film offered good matrix for adhesion and proliferation of L929 fibroblasts cells as indicated by the cell attachment study, FE-SEM of cell-film constructs and cytocompatibility assay.
32905737	3	33	contain	have	512:515	arg2	porosity					519:526	a porosity	517:526	a porosity of 95.3%	517:535	The composite films have a porosity of 95.3%, and showed good hydrophilicity with swelling ratio of 19 ± 1.8 and in vitro degradability.
32905737	3	33	contain	have	512:515	arg1	films					506:510	The composite films	492:510	The composite films	492:510	The composite films have a porosity of 95.3%, and showed good hydrophilicity with swelling ratio of 19 ± 1.8 and in vitro degradability.
32905737	2	34	theme	nano-biocomposite	390:406	arg1	films					408:412	the nano-biocomposite films	386:412	the nano-biocomposite films	386:412	Scanning electron microscopy (SEM) images of the nano-biocomposite films revealed the presence of interconnected pores, with fibre diameter 20-150 nm.
32905737	2	35	theme	fibre	466:470	arg1	diameter					472:479	fibre diameter 20-150 nm	466:489	fibre diameter 20-150 nm	466:489	Scanning electron microscopy (SEM) images of the nano-biocomposite films revealed the presence of interconnected pores, with fibre diameter 20-150 nm.
32905737	6	36	theme	paramount	1108:1116	arg1	importance					1118:1127	paramount importance	1108:1127	paramount importance	1108:1127	Thus, the nano-biocomposite films of BC-C-G could be of paramount importance as tissue engineering scaffold.
32905737	7	37	theme	polymer	1187:1193	arg1	films					1205:1209	The "all-natural" ternary polymer composite films	1161:1209	The "all-natural" ternary polymer composite films of BC-C-G	1161:1219	The "all-natural" ternary polymer composite films of BC-C-G have not been evaluated before for biomedical applications.
32905737	0	38	theme	synergistic	38:48	arg1	combination					50:60	synergistic combination	38:60	synergistic combination of bacterial cellulose with chitosan and gelatin for tissue engineering applications	38:145	Ternary nano-biocomposite films using synergistic combination of bacterial cellulose with chitosan and gelatin for tissue engineering applications.
32905737	6	39	theme	BC-C-G	1089:1094	arg1	films					1080:1084	the nano-biocomposite films	1058:1084	the nano-biocomposite films of BC-C-G	1058:1094	Thus, the nano-biocomposite films of BC-C-G could be of paramount importance as tissue engineering scaffold.
32905737	0	40	theme	bacterial	65:73	arg1	cellulose					75:83	bacterial cellulose	65:83	bacterial cellulose	65:83	Ternary nano-biocomposite films using synergistic combination of bacterial cellulose with chitosan and gelatin for tissue engineering applications.
32905737	5	41	theme	fibroblasts	931:941	arg1	cells					943:947	L929 fibroblasts cells	926:947	L929 fibroblasts cells	926:947	The composite film offered good matrix for adhesion and proliferation of L929 fibroblasts cells as indicated by the cell attachment study, FE-SEM of cell-film constructs and cytocompatibility assay.
32905737	5	42	theme	constructs	1012:1021	arg1	FE-SEM					992:997	FE-SEM	992:997	FE-SEM of cell-film constructs	992:1021	The composite film offered good matrix for adhesion and proliferation of L929 fibroblasts cells as indicated by the cell attachment study, FE-SEM of cell-film constructs and cytocompatibility assay.
32905737	5	42	theme	constructs	1012:1021	arg1	study					985:989	the cell attachment study	965:989	the cell attachment study	965:989	The composite film offered good matrix for adhesion and proliferation of L929 fibroblasts cells as indicated by the cell attachment study, FE-SEM of cell-film constructs and cytocompatibility assay.
32905737	5	42	theme	constructs	1012:1021	arg1	assay					1045:1049	cytocompatibility assay	1027:1049	cytocompatibility assay	1027:1049	The composite film offered good matrix for adhesion and proliferation of L929 fibroblasts cells as indicated by the cell attachment study, FE-SEM of cell-film constructs and cytocompatibility assay.
32905737	3	43	theme	%	535:535	arg1	porosity					519:526	a porosity	517:526	a porosity of 95.3%	517:535	The composite films have a porosity of 95.3%, and showed good hydrophilicity with swelling ratio of 19 ± 1.8 and in vitro degradability.
32905737	7	44	theme	composite	1195:1203	arg1	films					1205:1209	The "all-natural" ternary polymer composite films	1161:1209	The "all-natural" ternary polymer composite films of BC-C-G	1161:1219	The "all-natural" ternary polymer composite films of BC-C-G have not been evaluated before for biomedical applications.
32905737	4	45	dep	Fourier	677:683	arg1	transform					685:693	transform	685:693	transform infrared spectroscopy	685:715	X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, and thermogravimetric (TG) analysis results showed some interactions among the molecules of BC, gelatin, and chitosan within the film.
32905737	4	46	dep	transform	685:693	arg1	infrared					695:702	infrared	695:702	transform infrared spectroscopy	685:715	X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, and thermogravimetric (TG) analysis results showed some interactions among the molecules of BC, gelatin, and chitosan within the film.
32905737	1	47	theme	Ternary	148:154	arg1	films					174:178	Ternary nano-biocomposite films	148:178	Ternary nano-biocomposite films of bacterial cellulose-chitosan-gelatin (BC-C-G)	148:227	Ternary nano-biocomposite films of bacterial cellulose-chitosan-gelatin (BC-C-G) were fabricated by immersing the BC pellicles into chitosan and gelatin mixture and subsequently freeze-drying.
32905737	1	48	theme	BC	262:263	arg1	pellicles					265:273	the BC pellicles	258:273	the BC pellicles	258:273	Ternary nano-biocomposite films of bacterial cellulose-chitosan-gelatin (BC-C-G) were fabricated by immersing the BC pellicles into chitosan and gelatin mixture and subsequently freeze-drying.
32905737	5	49	theme	cell-film	1002:1010	arg1	constructs					1012:1021	cell-film constructs	1002:1021	cell-film constructs	1002:1021	The composite film offered good matrix for adhesion and proliferation of L929 fibroblasts cells as indicated by the cell attachment study, FE-SEM of cell-film constructs and cytocompatibility assay.
32905737	4	50	theme	BC	810:811	arg1	molecules					797:805	the molecules	793:805	the molecules of BC, gelatin, and chitosan	793:834	X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, and thermogravimetric (TG) analysis results showed some interactions among the molecules of BC, gelatin, and chitosan within the film.
32905737	1	51	theme	nano-biocomposite	156:172	arg1	films					174:178	Ternary nano-biocomposite films	148:178	Ternary nano-biocomposite films of bacterial cellulose-chitosan-gelatin (BC-C-G)	148:227	Ternary nano-biocomposite films of bacterial cellulose-chitosan-gelatin (BC-C-G) were fabricated by immersing the BC pellicles into chitosan and gelatin mixture and subsequently freeze-drying.
32905737	4	52	theme	attenuated	648:657	arg1	Fourier					677:683	attenuated total reflectance Fourier	648:683	attenuated total reflectance Fourier transform infrared spectroscopy	648:715	X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, and thermogravimetric (TG) analysis results showed some interactions among the molecules of BC, gelatin, and chitosan within the film.
32905737	5	53	theme	cells	943:947	arg1	proliferation					909:921	proliferation	909:921	proliferation	909:921	The composite film offered good matrix for adhesion and proliferation of L929 fibroblasts cells as indicated by the cell attachment study, FE-SEM of cell-film constructs and cytocompatibility assay.
32905737	5	53	theme	cells	943:947	arg1	adhesion					896:903	adhesion	896:903	adhesion	896:903	The composite film offered good matrix for adhesion and proliferation of L929 fibroblasts cells as indicated by the cell attachment study, FE-SEM of cell-film constructs and cytocompatibility assay.
32905737	3	54	theme	good	549:552	arg1	hydrophilicity					554:567	good hydrophilicity	549:567	good hydrophilicity	549:567	The composite films have a porosity of 95.3%, and showed good hydrophilicity with swelling ratio of 19 ± 1.8 and in vitro degradability.
32905737	3	55	theme	composite	496:504	arg1	films					506:510	The composite films	492:510	The composite films	492:510	The composite films have a porosity of 95.3%, and showed good hydrophilicity with swelling ratio of 19 ± 1.8 and in vitro degradability.
32905737	6	56	theme	nano-biocomposite	1062:1078	arg1	films					1080:1084	the nano-biocomposite films	1058:1084	the nano-biocomposite films of BC-C-G	1058:1094	Thus, the nano-biocomposite films of BC-C-G could be of paramount importance as tissue engineering scaffold.
32905737	0	57	theme	cellulose	75:83	arg1	combination					50:60	synergistic combination	38:60	synergistic combination of bacterial cellulose with chitosan and gelatin for tissue engineering applications	38:145	Ternary nano-biocomposite films using synergistic combination of bacterial cellulose with chitosan and gelatin for tissue engineering applications.
32905737	0	58	with	combination	50:60	arg1	chitosan					90:97	chitosan	90:97	chitosan	90:97	Ternary nano-biocomposite films using synergistic combination of bacterial cellulose with chitosan and gelatin for tissue engineering applications.
32905737	0	58	with	combination	50:60	arg1	gelatin					103:109	gelatin	103:109	gelatin	103:109	Ternary nano-biocomposite films using synergistic combination of bacterial cellulose with chitosan and gelatin for tissue engineering applications.
32905737	4	59	theme	total	659:663	arg1	Fourier					677:683	attenuated total reflectance Fourier	648:683	attenuated total reflectance Fourier transform infrared spectroscopy	648:715	X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, and thermogravimetric (TG) analysis results showed some interactions among the molecules of BC, gelatin, and chitosan within the film.
32905737	4	60	theme	reflectance	665:675	arg1	Fourier					677:683	attenuated total reflectance Fourier	648:683	attenuated total reflectance Fourier transform infrared spectroscopy	648:715	X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, and thermogravimetric (TG) analysis results showed some interactions among the molecules of BC, gelatin, and chitosan within the film.
34052274	3	0	theme	samples	668:674	arg1	surfaces					676:683	samples surfaces	668:683	samples surfaces	668:683	The antibacterial potentials of the samples were tested against Escherichia coli, Klebsiella sp., Pseudomonas aeruginosa A, and Pseudomonas aeruginosa B. SEM results revealed different changes in samples surfaces as a result of chemical modification.
34052274	5	1	theme	CHT	838:840	arg1	region					828:833	the crystalline region	812:833	the crystalline region of CHT	812:840	XRD spectra of CMC showed that the crystalline region of CHT was reduced by the modification.
34052274	6	2	theme	bactericidal	947:958	arg1	effects					960:966	inhibitory and bactericidal effects	932:966	inhibitory and bactericidal effects	932:966	The antibacterial results indicated that the samples had inhibitory and bactericidal effects against Escherichia coli and Klebsiella sp.
34052274	2	3	theme	X-ray	447:451	arg1	XRD					466:468	XRD	466:468	XRD	466:468	All the samples were characterized using Fourier Transform Infrared (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD).
34052274	2	3	theme	X-ray	447:451	arg1	diffraction					453:463	X-ray diffraction	447:463	X-ray diffraction (XRD)	447:469	All the samples were characterized using Fourier Transform Infrared (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD).
34052274	3	4	theme	different	647:655	arg1	changes					657:663	different changes	647:663	different changes in samples surfaces	647:683	The antibacterial potentials of the samples were tested against Escherichia coli, Klebsiella sp., Pseudomonas aeruginosa A, and Pseudomonas aeruginosa B. SEM results revealed different changes in samples surfaces as a result of chemical modification.
34052274	3	4	theme	different	647:655	arg1	result					690:695	a result	688:695	a result of chemical modification	688:720	The antibacterial potentials of the samples were tested against Escherichia coli, Klebsiella sp., Pseudomonas aeruginosa A, and Pseudomonas aeruginosa B. SEM results revealed different changes in samples surfaces as a result of chemical modification.
34052274	4	5	from	presence	740:747	arg1	composites					769:778	the composites	765:778	the composites	765:778	EDS revealed the presence of Ni and Cu in the composites.
34052274	6	6	theme	inhibitory	932:941	arg1	effects					960:966	inhibitory and bactericidal effects	932:966	inhibitory and bactericidal effects	932:966	The antibacterial results indicated that the samples had inhibitory and bactericidal effects against Escherichia coli and Klebsiella sp.
34052274	3	7	theme	samples	508:514	arg1	potentials					490:499	The antibacterial potentials	472:499	The antibacterial potentials of the samples	472:514	The antibacterial potentials of the samples were tested against Escherichia coli, Klebsiella sp., Pseudomonas aeruginosa A, and Pseudomonas aeruginosa B. SEM results revealed different changes in samples surfaces as a result of chemical modification.
34052274	2	8	dep	Infrared	347:354	arg1	FTIR					357:360	FTIR	357:360	FTIR	357:360	All the samples were characterized using Fourier Transform Infrared (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD).
34052274	0	9	theme	antibacterial	110:122	arg1	potential					124:132	antibacterial potential	110:132	antibacterial potential	110:132	Comparative studies of chitosan and carboxymethyl chitosan doped with nickel and copper: Characterization and antibacterial potential.
34052274	0	10	dep	Characterization	89:104	arg1	studies					12:18	Comparative studies	0:18	Comparative studies of chitosan and carboxymethyl chitosan doped with nickel and copper	0:86	Comparative studies of chitosan and carboxymethyl chitosan doped with nickel and copper: Characterization and antibacterial potential.
34052274	3	11	dep	coli	548:551	arg1	revealed					638:645	revealed	638:645	revealed different changes in samples surfaces as a result of chemical modification	638:720	The antibacterial potentials of the samples were tested against Escherichia coli, Klebsiella sp., Pseudomonas aeruginosa A, and Pseudomonas aeruginosa B. SEM results revealed different changes in samples surfaces as a result of chemical modification.
34052274	8	12	theme	CHT	1159:1161	arg1	composites					1177:1186	CHT and CHT-metal composites	1159:1186	CHT and CHT-metal composites	1159:1186	The study showed that CMC and CMC-metal composites performed better at inhibiting the growth of microorganisms than CHT and CHT-metal composites.
34052274	2	13	theme	X-ray	418:422	arg1	EDS					438:440	EDS	438:440	EDS	438:440	All the samples were characterized using Fourier Transform Infrared (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD).
34052274	2	13	theme	X-ray	418:422	arg1	spectroscopy					424:435	energy dispersive X-ray spectroscopy	400:435	energy dispersive X-ray spectroscopy (EDS)	400:441	All the samples were characterized using Fourier Transform Infrared (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD).
34052274	1	14	theme	antibacterial	176:188	arg1	evaluation					190:199	antibacterial evaluation	176:199	antibacterial evaluation	176:199	The study focused on the preparation and antibacterial evaluation of chitosan (CHT), carboxymethyl chitosan (CMC) and their respective metal composites.
34052274	4	15	attach	presence	740:747	arg2	Cu					759:760	Cu	759:760	Cu	759:760	EDS revealed the presence of Ni and Cu in the composites.
34052274	4	15	attach	presence	740:747	arg1	composites					769:778	the composites	765:778	the composites	765:778	EDS revealed the presence of Ni and Cu in the composites.
34052274	4	15	attach	presence	740:747	arg2	Ni					752:753	Ni	752:753	Ni	752:753	EDS revealed the presence of Ni and Cu in the composites.
34052274	3	16	theme	modification	709:720	arg1	changes					657:663	different changes	647:663	different changes in samples surfaces	647:683	The antibacterial potentials of the samples were tested against Escherichia coli, Klebsiella sp., Pseudomonas aeruginosa A, and Pseudomonas aeruginosa B. SEM results revealed different changes in samples surfaces as a result of chemical modification.
34052274	3	16	theme	modification	709:720	arg1	result					690:695	a result	688:695	a result of chemical modification	688:720	The antibacterial potentials of the samples were tested against Escherichia coli, Klebsiella sp., Pseudomonas aeruginosa A, and Pseudomonas aeruginosa B. SEM results revealed different changes in samples surfaces as a result of chemical modification.
34052274	6	17	contain	had	928:930	arg2	effects					960:966	inhibitory and bactericidal effects	932:966	inhibitory and bactericidal effects	932:966	The antibacterial results indicated that the samples had inhibitory and bactericidal effects against Escherichia coli and Klebsiella sp.
34052274	6	17	contain	had	928:930	arg1	samples					920:926	the samples	916:926	the samples	916:926	The antibacterial results indicated that the samples had inhibitory and bactericidal effects against Escherichia coli and Klebsiella sp.
34052274	2	18	theme	scanning	364:371	arg1	SEM					394:396	SEM	394:396	SEM	394:396	All the samples were characterized using Fourier Transform Infrared (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD).
34052274	2	18	theme	scanning	364:371	arg1	microscopy					382:391	scanning electron microscopy	364:391	scanning electron microscopy (SEM)	364:397	All the samples were characterized using Fourier Transform Infrared (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD).
34052274	0	19	theme	Comparative	0:10	arg1	studies					12:18	Comparative studies	0:18	Comparative studies of chitosan and carboxymethyl chitosan doped with nickel and copper	0:86	Comparative studies of chitosan and carboxymethyl chitosan doped with nickel and copper: Characterization and antibacterial potential.
34052274	1	20	theme	respective	259:268	arg1	composites					276:285	their respective metal composites	253:285	their respective metal composites	253:285	The study focused on the preparation and antibacterial evaluation of chitosan (CHT), carboxymethyl chitosan (CMC) and their respective metal composites.
34052274	1	21	dep	preparation	160:170	arg1	the					156:158	the	156:158	the	156:158	The study focused on the preparation and antibacterial evaluation of chitosan (CHT), carboxymethyl chitosan (CMC) and their respective metal composites.
34052274	5	22	theme	crystalline	816:826	arg1	region					828:833	the crystalline region	812:833	the crystalline region of CHT	812:840	XRD spectra of CMC showed that the crystalline region of CHT was reduced by the modification.
34052274	0	23	theme	chitosan	23:30	arg1	studies					12:18	Comparative studies	0:18	Comparative studies of chitosan and carboxymethyl chitosan doped with nickel and copper	0:86	Comparative studies of chitosan and carboxymethyl chitosan doped with nickel and copper: Characterization and antibacterial potential.
34052274	1	24	theme	metal	270:274	arg1	composites					276:285	their respective metal composites	253:285	their respective metal composites	253:285	The study focused on the preparation and antibacterial evaluation of chitosan (CHT), carboxymethyl chitosan (CMC) and their respective metal composites.
34052274	8	25	theme	microorganisms	1139:1152	arg1	growth					1129:1134	the growth	1125:1134	the growth of microorganisms	1125:1152	The study showed that CMC and CMC-metal composites performed better at inhibiting the growth of microorganisms than CHT and CHT-metal composites.
34052274	6	26	theme	Klebsiella	997:1006	arg1	sp					1008:1009	Klebsiella sp	997:1009	Klebsiella sp	997:1009	The antibacterial results indicated that the samples had inhibitory and bactericidal effects against Escherichia coli and Klebsiella sp.
34052274	1	27	theme	chitosan	204:211	arg1	evaluation					190:199	antibacterial evaluation	176:199	antibacterial evaluation	176:199	The study focused on the preparation and antibacterial evaluation of chitosan (CHT), carboxymethyl chitosan (CMC) and their respective metal composites.
34052274	1	27	theme	chitosan	204:211	arg1	preparation					160:170	preparation	160:170	preparation	160:170	The study focused on the preparation and antibacterial evaluation of chitosan (CHT), carboxymethyl chitosan (CMC) and their respective metal composites.
34052274	2	28	theme	energy	400:405	arg1	EDS					438:440	EDS	438:440	EDS	438:440	All the samples were characterized using Fourier Transform Infrared (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD).
34052274	2	28	theme	energy	400:405	arg1	spectroscopy					424:435	energy dispersive X-ray spectroscopy	400:435	energy dispersive X-ray spectroscopy (EDS)	400:441	All the samples were characterized using Fourier Transform Infrared (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD).
34052274	1	29	theme	composites	276:285	arg1	evaluation					190:199	antibacterial evaluation	176:199	antibacterial evaluation	176:199	The study focused on the preparation and antibacterial evaluation of chitosan (CHT), carboxymethyl chitosan (CMC) and their respective metal composites.
34052274	1	29	theme	composites	276:285	arg1	preparation					160:170	preparation	160:170	preparation	160:170	The study focused on the preparation and antibacterial evaluation of chitosan (CHT), carboxymethyl chitosan (CMC) and their respective metal composites.
34052274	3	30	theme	Pseudomonas	600:610	arg1	SEM					626:628	Pseudomonas aeruginosa B. SEM	600:628	Pseudomonas aeruginosa B. SEM	600:628	The antibacterial potentials of the samples were tested against Escherichia coli, Klebsiella sp., Pseudomonas aeruginosa A, and Pseudomonas aeruginosa B. SEM results revealed different changes in samples surfaces as a result of chemical modification.
34052274	2	31	dep	Fourier	329:335	arg1	Transform					337:345	Transform	337:345	Transform	337:345	All the samples were characterized using Fourier Transform Infrared (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD).
34052274	0	32	theme	carboxymethyl	36:48	arg1	chitosan					50:57	carboxymethyl chitosan	36:57	carboxymethyl chitosan	36:57	Comparative studies of chitosan and carboxymethyl chitosan doped with nickel and copper: Characterization and antibacterial potential.
34052274	3	33	theme	chemical	700:707	arg1	modification					709:720	chemical modification	700:720	chemical modification	700:720	The antibacterial potentials of the samples were tested against Escherichia coli, Klebsiella sp., Pseudomonas aeruginosa A, and Pseudomonas aeruginosa B. SEM results revealed different changes in samples surfaces as a result of chemical modification.
34052274	3	34	theme	aeruginosa	612:621	arg1	SEM					626:628	Pseudomonas aeruginosa B. SEM	600:628	Pseudomonas aeruginosa B. SEM	600:628	The antibacterial potentials of the samples were tested against Escherichia coli, Klebsiella sp., Pseudomonas aeruginosa A, and Pseudomonas aeruginosa B. SEM results revealed different changes in samples surfaces as a result of chemical modification.
34052274	3	35	dep	aeruginosa	582:591	arg1	A					593:593	A	593:593	Pseudomonas aeruginosa A	570:593	The antibacterial potentials of the samples were tested against Escherichia coli, Klebsiella sp., Pseudomonas aeruginosa A, and Pseudomonas aeruginosa B. SEM results revealed different changes in samples surfaces as a result of chemical modification.
34052274	3	36	from	changes	657:663	arg1	surfaces					676:683	samples surfaces	668:683	samples surfaces	668:683	The antibacterial potentials of the samples were tested against Escherichia coli, Klebsiella sp., Pseudomonas aeruginosa A, and Pseudomonas aeruginosa B. SEM results revealed different changes in samples surfaces as a result of chemical modification.
34052274	8	37	theme	CMC-metal	1073:1081	arg1	composites					1083:1092	CMC-metal composites	1073:1092	CMC-metal composites	1073:1092	The study showed that CMC and CMC-metal composites performed better at inhibiting the growth of microorganisms than CHT and CHT-metal composites.
34052274	2	38	theme	dispersive	407:416	arg1	EDS					438:440	EDS	438:440	EDS	438:440	All the samples were characterized using Fourier Transform Infrared (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD).
34052274	2	38	theme	dispersive	407:416	arg1	spectroscopy					424:435	energy dispersive X-ray spectroscopy	400:435	energy dispersive X-ray spectroscopy (EDS)	400:441	All the samples were characterized using Fourier Transform Infrared (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD).
34052274	5	39	theme	CMC	796:798	arg1	spectra					785:791	XRD spectra	781:791	XRD spectra of CMC	781:798	XRD spectra of CMC showed that the crystalline region of CHT was reduced by the modification.
34052274	8	40	theme	CHT-metal	1167:1175	arg1	composites					1177:1186	CHT and CHT-metal composites	1159:1186	CHT and CHT-metal composites	1159:1186	The study showed that CMC and CMC-metal composites performed better at inhibiting the growth of microorganisms than CHT and CHT-metal composites.
34052274	6	41	theme	antibacterial	879:891	arg1	results					893:899	The antibacterial results	875:899	The antibacterial results	875:899	The antibacterial results indicated that the samples had inhibitory and bactericidal effects against Escherichia coli and Klebsiella sp.
34052274	0	42	theme	chitosan	50:57	arg1	studies					12:18	Comparative studies	0:18	Comparative studies of chitosan and carboxymethyl chitosan doped with nickel and copper	0:86	Comparative studies of chitosan and carboxymethyl chitosan doped with nickel and copper: Characterization and antibacterial potential.
34052274	4	43	theme	Ni	752:753	arg1	presence					740:747	the presence	736:747	the presence of Ni and Cu in the composites	736:778	EDS revealed the presence of Ni and Cu in the composites.
34052274	3	44	theme	B.	623:624	arg1	SEM					626:628	Pseudomonas aeruginosa B. SEM	600:628	Pseudomonas aeruginosa B. SEM	600:628	The antibacterial potentials of the samples were tested against Escherichia coli, Klebsiella sp., Pseudomonas aeruginosa A, and Pseudomonas aeruginosa B. SEM results revealed different changes in samples surfaces as a result of chemical modification.
34052274	1	45	theme	carboxymethyl	220:232	arg1	chitosan					234:241	carboxymethyl chitosan	220:241	carboxymethyl chitosan (CMC)	220:247	The study focused on the preparation and antibacterial evaluation of chitosan (CHT), carboxymethyl chitosan (CMC) and their respective metal composites.
34052274	1	45	theme	carboxymethyl	220:232	arg1	CMC					244:246	CMC	244:246	CMC	244:246	The study focused on the preparation and antibacterial evaluation of chitosan (CHT), carboxymethyl chitosan (CMC) and their respective metal composites.
34052274	4	46	theme	Cu	759:760	arg1	presence					740:747	the presence	736:747	the presence of Ni and Cu in the composites	736:778	EDS revealed the presence of Ni and Cu in the composites.
34052274	5	47	theme	XRD	781:783	arg1	spectra					785:791	XRD spectra	781:791	XRD spectra of CMC	781:798	XRD spectra of CMC showed that the crystalline region of CHT was reduced by the modification.
34052274	2	48	theme	electron	373:380	arg1	SEM					394:396	SEM	394:396	SEM	394:396	All the samples were characterized using Fourier Transform Infrared (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD).
34052274	2	48	theme	electron	373:380	arg1	microscopy					382:391	scanning electron microscopy	364:391	scanning electron microscopy (SEM)	364:397	All the samples were characterized using Fourier Transform Infrared (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD).
34052274	1	49	theme	chitosan	234:241	arg1	evaluation					190:199	antibacterial evaluation	176:199	antibacterial evaluation	176:199	The study focused on the preparation and antibacterial evaluation of chitosan (CHT), carboxymethyl chitosan (CMC) and their respective metal composites.
34052274	1	49	theme	chitosan	234:241	arg1	preparation					160:170	preparation	160:170	preparation	160:170	The study focused on the preparation and antibacterial evaluation of chitosan (CHT), carboxymethyl chitosan (CMC) and their respective metal composites.
34052274	3	50	theme	antibacterial	476:488	arg1	potentials					490:499	The antibacterial potentials	472:499	The antibacterial potentials of the samples	472:514	The antibacterial potentials of the samples were tested against Escherichia coli, Klebsiella sp., Pseudomonas aeruginosa A, and Pseudomonas aeruginosa B. SEM results revealed different changes in samples surfaces as a result of chemical modification.
32718619	5	0	theme	GO	1110:1111	arg1	introduction					1080:1091	the introduction	1076:1091	the introduction	1076:1091	Moreover, the nanocomposites showed excellent fire retardant property with a limiting oxygen index of 46.8 % due to the introduction of bentonite and GO, which shows potential application in fire-protective insulation, packaging and coating.
32718619	5	1	from	application	1136:1146	arg1	insulation					1167:1176	fire-protective insulation	1151:1176	fire-protective insulation	1151:1176	Moreover, the nanocomposites showed excellent fire retardant property with a limiting oxygen index of 46.8 % due to the introduction of bentonite and GO, which shows potential application in fire-protective insulation, packaging and coating.
32718619	5	1	from	application	1136:1146	arg1	coating					1193:1199	coating	1193:1199	coating	1193:1199	Moreover, the nanocomposites showed excellent fire retardant property with a limiting oxygen index of 46.8 % due to the introduction of bentonite and GO, which shows potential application in fire-protective insulation, packaging and coating.
32718619	5	1	from	application	1136:1146	arg1	packaging					1179:1187	packaging	1179:1187	packaging	1179:1187	Moreover, the nanocomposites showed excellent fire retardant property with a limiting oxygen index of 46.8 % due to the introduction of bentonite and GO, which shows potential application in fire-protective insulation, packaging and coating.
32718619	4	2	theme	MPa	856:858	arg1	composite					786:794	a composite	784:794	a composite possessing a maximum tensile stress	784:830	This synergistic strengthen effect resulted in a composite possessing a maximum tensile stress and toughness of 231.16 MPa and 4.53 MJ/m3, respectively, harder than most of the previously reported hemicellulose composites.
32718619	4	2	theme	MPa	856:858	arg1	toughness					836:844	toughness	836:844	toughness	836:844	This synergistic strengthen effect resulted in a composite possessing a maximum tensile stress and toughness of 231.16 MPa and 4.53 MJ/m3, respectively, harder than most of the previously reported hemicellulose composites.
32718619	4	2	theme	MPa	856:858	arg1	MPa					856:858	231.16 MPa	849:858	231.16 MPa	849:858	This synergistic strengthen effect resulted in a composite possessing a maximum tensile stress and toughness of 231.16 MPa and 4.53 MJ/m3, respectively, harder than most of the previously reported hemicellulose composites.
32718619	4	2	theme	MPa	856:858	arg1	MJ/m3					869:873	4.53 MJ/m3	864:873	4.53 MJ/m3	864:873	This synergistic strengthen effect resulted in a composite possessing a maximum tensile stress and toughness of 231.16 MPa and 4.53 MJ/m3, respectively, harder than most of the previously reported hemicellulose composites.
32718619	1	3	from	materials	186:194	arg1	industries					207:216	various industries	199:216	various industries	199:216	With the great demand for high-strength integrated materials in various industries, products from renewable resources were expected to replace petroleum-based materials.
32718619	2	4	theme	graphene	382:389	arg1	oxide					391:395	graphene oxide	382:395	graphene oxide (GO)	382:400	Inspired by the hierarchical structure of nacre, in this work, bentonite and graphene oxide (GO) were incorporated into the galactomannan (GM) matrix to prepare a ternary nanocomposite, which was further cross-linked and strengthened with borate.
32718619	4	5	theme	reported	925:932	arg1	composites					948:957	the previously reported hemicellulose composites	910:957	the previously reported hemicellulose composites	910:957	This synergistic strengthen effect resulted in a composite possessing a maximum tensile stress and toughness of 231.16 MPa and 4.53 MJ/m3, respectively, harder than most of the previously reported hemicellulose composites.
32718619	2	6	dep	oxide	391:395	arg1	GO					398:399	GO	398:399	GO	398:399	Inspired by the hierarchical structure of nacre, in this work, bentonite and graphene oxide (GO) were incorporated into the galactomannan (GM) matrix to prepare a ternary nanocomposite, which was further cross-linked and strengthened with borate.
32718619	0	7	theme	borate	113:118	arg1	cross-linking					120:132	borate cross-linking	113:132	borate cross-linking	113:132	Biomimetic galactomannan/bentonite/graphene oxide film with superior mechanical and fire retardant properties by borate cross-linking.
32718619	4	8	theme	synergistic	742:752	arg1	effect					765:770	This synergistic strengthen effect	737:770	This synergistic strengthen effect	737:770	This synergistic strengthen effect resulted in a composite possessing a maximum tensile stress and toughness of 231.16 MPa and 4.53 MJ/m3, respectively, harder than most of the previously reported hemicellulose composites.
32718619	2	9	theme	hierarchical	321:332	arg1	structure					334:342	the hierarchical structure	317:342	the hierarchical structure of nacre	317:351	Inspired by the hierarchical structure of nacre, in this work, bentonite and graphene oxide (GO) were incorporated into the galactomannan (GM) matrix to prepare a ternary nanocomposite, which was further cross-linked and strengthened with borate.
32718619	1	10	theme	various	199:205	arg1	industries					207:216	various industries	199:216	various industries	199:216	With the great demand for high-strength integrated materials in various industries, products from renewable resources were expected to replace petroleum-based materials.
32718619	4	11	theme	hemicellulose	934:946	arg1	composites					948:957	the previously reported hemicellulose composites	910:957	the previously reported hemicellulose composites	910:957	This synergistic strengthen effect resulted in a composite possessing a maximum tensile stress and toughness of 231.16 MPa and 4.53 MJ/m3, respectively, harder than most of the previously reported hemicellulose composites.
32718619	0	12	theme	galactomannan/bentonite/graphene	11:42	arg1	film					50:53	Biomimetic galactomannan/bentonite/graphene oxide film	0:53	Biomimetic galactomannan/bentonite/graphene oxide film with superior mechanical and fire retardant properties by borate cross-linking.	0:133	Biomimetic galactomannan/bentonite/graphene oxide film with superior mechanical and fire retardant properties by borate cross-linking.
32718619	5	13	theme	bentonite	1096:1104	arg1	introduction					1080:1091	the introduction	1076:1091	the introduction	1076:1091	Moreover, the nanocomposites showed excellent fire retardant property with a limiting oxygen index of 46.8 % due to the introduction of bentonite and GO, which shows potential application in fire-protective insulation, packaging and coating.
32718619	3	14	theme	composite	582:590	arg1	structure					565:573	The chemical structure	552:573	The chemical structure of the composite	552:590	The chemical structure of the composite was analyzed with SEM, FTIR, XPS and XRD, revealing a co-assembly reaction between GO, bentonite and GM, accompanied by the borate crosslinking.
32718619	0	15	theme	Biomimetic	0:9	arg1	film					50:53	Biomimetic galactomannan/bentonite/graphene oxide film	0:53	Biomimetic galactomannan/bentonite/graphene oxide film with superior mechanical and fire retardant properties by borate cross-linking.	0:133	Biomimetic galactomannan/bentonite/graphene oxide film with superior mechanical and fire retardant properties by borate cross-linking.
32718619	5	16	theme	fire-protective	1151:1165	arg1	insulation					1167:1176	fire-protective insulation	1151:1176	fire-protective insulation	1151:1176	Moreover, the nanocomposites showed excellent fire retardant property with a limiting oxygen index of 46.8 % due to the introduction of bentonite and GO, which shows potential application in fire-protective insulation, packaging and coating.
32718619	5	17	theme	retardant	1011:1019	arg1	property					1021:1028	excellent fire retardant property	996:1028	excellent fire retardant property	996:1028	Moreover, the nanocomposites showed excellent fire retardant property with a limiting oxygen index of 46.8 % due to the introduction of bentonite and GO, which shows potential application in fire-protective insulation, packaging and coating.
32718619	2	18	theme	GM	444:445	arg1	matrix					448:453	the galactomannan (GM) matrix	425:453	the galactomannan (GM) matrix	425:453	Inspired by the hierarchical structure of nacre, in this work, bentonite and graphene oxide (GO) were incorporated into the galactomannan (GM) matrix to prepare a ternary nanocomposite, which was further cross-linked and strengthened with borate.
32718619	0	19	theme	oxide	44:48	arg1	film					50:53	Biomimetic galactomannan/bentonite/graphene oxide film	0:53	Biomimetic galactomannan/bentonite/graphene oxide film with superior mechanical and fire retardant properties by borate cross-linking.	0:133	Biomimetic galactomannan/bentonite/graphene oxide film with superior mechanical and fire retardant properties by borate cross-linking.
32718619	3	20	theme	borate	716:721	arg1	crosslinking					723:734	the borate crosslinking	712:734	the borate crosslinking	712:734	The chemical structure of the composite was analyzed with SEM, FTIR, XPS and XRD, revealing a co-assembly reaction between GO, bentonite and GM, accompanied by the borate crosslinking.
32718619	2	21	theme	nacre	347:351	arg1	structure					334:342	the hierarchical structure	317:342	the hierarchical structure of nacre	317:351	Inspired by the hierarchical structure of nacre, in this work, bentonite and graphene oxide (GO) were incorporated into the galactomannan (GM) matrix to prepare a ternary nanocomposite, which was further cross-linked and strengthened with borate.
32718619	1	22	theme	renewable	233:241	arg1	resources					243:251	renewable resources	233:251	renewable resources	233:251	With the great demand for high-strength integrated materials in various industries, products from renewable resources were expected to replace petroleum-based materials.
32718619	0	23	theme	superior	60:67	arg1	properties					99:108	superior mechanical and fire retardant properties	60:108	superior mechanical and fire retardant properties	60:108	Biomimetic galactomannan/bentonite/graphene oxide film with superior mechanical and fire retardant properties by borate cross-linking.
32718619	5	24	theme	%	1067:1067	arg1	index					1053:1057	a limiting oxygen index	1035:1057	a limiting oxygen index of 46.8 %	1035:1067	Moreover, the nanocomposites showed excellent fire retardant property with a limiting oxygen index of 46.8 % due to the introduction of bentonite and GO, which shows potential application in fire-protective insulation, packaging and coating.
32718619	4	25	theme	maximum	809:815	arg1	stress					825:830	a maximum tensile stress	807:830	a maximum tensile stress	807:830	This synergistic strengthen effect resulted in a composite possessing a maximum tensile stress and toughness of 231.16 MPa and 4.53 MJ/m3, respectively, harder than most of the previously reported hemicellulose composites.
32718619	2	26	theme	galactomannan	429:441	arg1	matrix					448:453	the galactomannan (GM) matrix	425:453	the galactomannan (GM) matrix	425:453	Inspired by the hierarchical structure of nacre, in this work, bentonite and graphene oxide (GO) were incorporated into the galactomannan (GM) matrix to prepare a ternary nanocomposite, which was further cross-linked and strengthened with borate.
32718619	0	27	with	film	50:53	arg1	properties					99:108	superior mechanical and fire retardant properties	60:108	superior mechanical and fire retardant properties	60:108	Biomimetic galactomannan/bentonite/graphene oxide film with superior mechanical and fire retardant properties by borate cross-linking.
32718619	2	28	theme	ternary	468:474	arg1	nanocomposite					476:488	a ternary nanocomposite	466:488	a ternary nanocomposite	466:488	Inspired by the hierarchical structure of nacre, in this work, bentonite and graphene oxide (GO) were incorporated into the galactomannan (GM) matrix to prepare a ternary nanocomposite, which was further cross-linked and strengthened with borate.
32718619	5	29	theme	potential	1126:1134	arg1	application					1136:1146	potential application	1126:1146	potential application in fire-protective insulation, packaging and coating	1126:1199	Moreover, the nanocomposites showed excellent fire retardant property with a limiting oxygen index of 46.8 % due to the introduction of bentonite and GO, which shows potential application in fire-protective insulation, packaging and coating.
32718619	0	30	theme	mechanical	69:78	arg1	properties					99:108	superior mechanical and fire retardant properties	60:108	superior mechanical and fire retardant properties	60:108	Biomimetic galactomannan/bentonite/graphene oxide film with superior mechanical and fire retardant properties by borate cross-linking.
32718619	5	31	theme	limiting	1037:1044	arg1	index					1053:1057	a limiting oxygen index	1035:1057	a limiting oxygen index of 46.8 %	1035:1067	Moreover, the nanocomposites showed excellent fire retardant property with a limiting oxygen index of 46.8 % due to the introduction of bentonite and GO, which shows potential application in fire-protective insulation, packaging and coating.
32718619	4	32	theme	tensile	817:823	arg1	stress					825:830	a maximum tensile stress	807:830	a maximum tensile stress	807:830	This synergistic strengthen effect resulted in a composite possessing a maximum tensile stress and toughness of 231.16 MPa and 4.53 MJ/m3, respectively, harder than most of the previously reported hemicellulose composites.
32718619	1	33	theme	great	144:148	arg1	demand					150:155	the great demand	140:155	the great demand for high-strength integrated materials in various industries	140:216	With the great demand for high-strength integrated materials in various industries, products from renewable resources were expected to replace petroleum-based materials.
32718619	5	34	theme	oxygen	1046:1051	arg1	index					1053:1057	a limiting oxygen index	1035:1057	a limiting oxygen index of 46.8 %	1035:1067	Moreover, the nanocomposites showed excellent fire retardant property with a limiting oxygen index of 46.8 % due to the introduction of bentonite and GO, which shows potential application in fire-protective insulation, packaging and coating.
32718619	3	35	theme	co-assembly	646:656	arg1	reaction					658:665	a co-assembly reaction	644:665	a co-assembly reaction between GO, bentonite and GM	644:694	The chemical structure of the composite was analyzed with SEM, FTIR, XPS and XRD, revealing a co-assembly reaction between GO, bentonite and GM, accompanied by the borate crosslinking.
32718619	1	36	from	resources	243:251	arg1	products					219:226	products	219:226	products from renewable resources	219:251	With the great demand for high-strength integrated materials in various industries, products from renewable resources were expected to replace petroleum-based materials.
32718619	4	37	dep	effect	765:770	arg1	strengthen					754:763	strengthen	754:763	strengthen	754:763	This synergistic strengthen effect resulted in a composite possessing a maximum tensile stress and toughness of 231.16 MPa and 4.53 MJ/m3, respectively, harder than most of the previously reported hemicellulose composites.
32718619	4	38	contain	possessing	796:805	arg1	composite					786:794	a composite	784:794	a composite possessing a maximum tensile stress	784:830	This synergistic strengthen effect resulted in a composite possessing a maximum tensile stress and toughness of 231.16 MPa and 4.53 MJ/m3, respectively, harder than most of the previously reported hemicellulose composites.
32718619	4	38	contain	possessing	796:805	arg2	stress					825:830	a maximum tensile stress	807:830	a maximum tensile stress	807:830	This synergistic strengthen effect resulted in a composite possessing a maximum tensile stress and toughness of 231.16 MPa and 4.53 MJ/m3, respectively, harder than most of the previously reported hemicellulose composites.
32718619	4	38	contain	possessing	796:805	arg1	MPa					856:858	231.16 MPa	849:858	231.16 MPa	849:858	This synergistic strengthen effect resulted in a composite possessing a maximum tensile stress and toughness of 231.16 MPa and 4.53 MJ/m3, respectively, harder than most of the previously reported hemicellulose composites.
32718619	0	39	theme	retardant	89:97	arg1	properties					99:108	superior mechanical and fire retardant properties	60:108	superior mechanical and fire retardant properties	60:108	Biomimetic galactomannan/bentonite/graphene oxide film with superior mechanical and fire retardant properties by borate cross-linking.
32718619	5	40	theme	excellent	996:1004	arg1	property					1021:1028	excellent fire retardant property	996:1028	excellent fire retardant property	996:1028	Moreover, the nanocomposites showed excellent fire retardant property with a limiting oxygen index of 46.8 % due to the introduction of bentonite and GO, which shows potential application in fire-protective insulation, packaging and coating.
32718619	1	41	theme	high-strength	161:173	arg1	materials					186:194	high-strength integrated materials	161:194	high-strength integrated materials in various industries	161:216	With the great demand for high-strength integrated materials in various industries, products from renewable resources were expected to replace petroleum-based materials.
32718619	0	42	theme	fire	84:87	arg1	properties					99:108	superior mechanical and fire retardant properties	60:108	superior mechanical and fire retardant properties	60:108	Biomimetic galactomannan/bentonite/graphene oxide film with superior mechanical and fire retardant properties by borate cross-linking.
32718619	3	43	theme	chemical	556:563	arg1	structure					565:573	The chemical structure	552:573	The chemical structure of the composite	552:590	The chemical structure of the composite was analyzed with SEM, FTIR, XPS and XRD, revealing a co-assembly reaction between GO, bentonite and GM, accompanied by the borate crosslinking.
32718619	5	44	theme	fire	1006:1009	arg1	property					1021:1028	excellent fire retardant property	996:1028	excellent fire retardant property	996:1028	Moreover, the nanocomposites showed excellent fire retardant property with a limiting oxygen index of 46.8 % due to the introduction of bentonite and GO, which shows potential application in fire-protective insulation, packaging and coating.
32718619	1	45	theme	integrated	175:184	arg1	materials					186:194	high-strength integrated materials	161:194	high-strength integrated materials in various industries	161:216	With the great demand for high-strength integrated materials in various industries, products from renewable resources were expected to replace petroleum-based materials.
32718619	1	46	theme	petroleum-based	278:292	arg1	materials					294:302	petroleum-based materials	278:302	petroleum-based materials	278:302	With the great demand for high-strength integrated materials in various industries, products from renewable resources were expected to replace petroleum-based materials.
34856163	8	0	theme	improved	1067:1074	arg1	stability					1076:1084	The improved stability	1063:1084	The improved stability	1063:1084	The improved stability and recyclability render the as-prepared immobilized phenol-degrading bacteria with great potential for industrial applications.
34856163	4	1	theme	cell	590:593	arg1	loading					595:601	high cell loading	585:601	high cell loading	585:601	pneumoniae composite bead showed good pelletizing properties, superior material strength and high cell loading.
34856163	4	1	theme	cell	590:593	arg1	properties					542:551	good pelletizing properties	525:551	good pelletizing properties	525:551	pneumoniae composite bead showed good pelletizing properties, superior material strength and high cell loading.
34856163	5	2	theme	Fe3O4-induced	743:755	arg1	·OH					776:778	·OH	776:778	·OH	776:778	Introduction of nano-Fe3O4 to the composite gel further enhanced phenol degradation rate by >10% owing to strengthened phenol oxidation by Fe3O4-induced hydroxyl radicals (·OH) and improved mass and electron transfers.
34856163	5	2	theme	Fe3O4-induced	743:755	arg1	radicals					766:773	Fe3O4-induced hydroxyl radicals	743:773	Fe3O4-induced hydroxyl radicals (·OH)	743:779	Introduction of nano-Fe3O4 to the composite gel further enhanced phenol degradation rate by >10% owing to strengthened phenol oxidation by Fe3O4-induced hydroxyl radicals (·OH) and improved mass and electron transfers.
34856163	8	3	theme	immobilized	1127:1137	arg1	bacteria					1156:1163	the as-prepared immobilized phenol-degrading bacteria	1111:1163	the as-prepared immobilized phenol-degrading bacteria	1111:1163	The improved stability and recyclability render the as-prepared immobilized phenol-degrading bacteria with great potential for industrial applications.
34856163	7	4	theme	highest	1001:1007	arg1	rate					1009:1012	the highest rate	997:1012	the highest rate of 55.12 mg L-1·h-1 obtained at the 15th cycles	997:1060	pneumoniae composite bead showed that 1500 mg/L phenol was fully degraded for all cycles with the highest rate of 55.12 mg L-1·h-1 obtained at the 15th cycles.
34856163	5	5	theme	hydroxyl	757:764	arg1	·OH					776:778	·OH	776:778	·OH	776:778	Introduction of nano-Fe3O4 to the composite gel further enhanced phenol degradation rate by >10% owing to strengthened phenol oxidation by Fe3O4-induced hydroxyl radicals (·OH) and improved mass and electron transfers.
34856163	5	5	theme	hydroxyl	757:764	arg1	radicals					766:773	Fe3O4-induced hydroxyl radicals	743:773	Fe3O4-induced hydroxyl radicals (·OH)	743:779	Introduction of nano-Fe3O4 to the composite gel further enhanced phenol degradation rate by >10% owing to strengthened phenol oxidation by Fe3O4-induced hydroxyl radicals (·OH) and improved mass and electron transfers.
34856163	8	6	theme	great	1170:1174	arg1	potential					1176:1184	great potential	1170:1184	great potential for industrial applications	1170:1212	The improved stability and recyclability render the as-prepared immobilized phenol-degrading bacteria with great potential for industrial applications.
34856163	8	7	theme	as-prepared	1115:1125	arg1	bacteria					1156:1163	the as-prepared immobilized phenol-degrading bacteria	1111:1163	the as-prepared immobilized phenol-degrading bacteria	1111:1163	The improved stability and recyclability render the as-prepared immobilized phenol-degrading bacteria with great potential for industrial applications.
34856163	2	8	theme	agar/κ-carrageenan	333:350	arg1	hydrogel					362:369	an agar/κ-carrageenan composite hydrogel	330:369	an agar/κ-carrageenan composite hydrogel to entrap Klebsiella pneumoniae with the combination of nano-Fe3O4 for processing phenol wastes	330:465	Herein, we developed an agar/κ-carrageenan composite hydrogel to entrap Klebsiella pneumoniae with the combination of nano-Fe3O4 for processing phenol wastes.
34856163	2	9	theme	composite	352:360	arg1	hydrogel					362:369	an agar/κ-carrageenan composite hydrogel	330:369	an agar/κ-carrageenan composite hydrogel to entrap Klebsiella pneumoniae with the combination of nano-Fe3O4 for processing phenol wastes	330:465	Herein, we developed an agar/κ-carrageenan composite hydrogel to entrap Klebsiella pneumoniae with the combination of nano-Fe3O4 for processing phenol wastes.
34856163	4	10	theme	pneumoniae	492:501	arg1	bead					513:516	pneumoniae composite bead	492:516	pneumoniae composite bead	492:516	pneumoniae composite bead showed good pelletizing properties, superior material strength and high cell loading.
34856163	4	11	theme	material	563:570	arg1	properties					542:551	good pelletizing properties	525:551	good pelletizing properties	525:551	pneumoniae composite bead showed good pelletizing properties, superior material strength and high cell loading.
34856163	4	11	theme	material	563:570	arg1	strength					572:579	superior material strength	554:579	superior material strength	554:579	pneumoniae composite bead showed good pelletizing properties, superior material strength and high cell loading.
34856163	5	12	theme	strengthened	710:721	arg1	oxidation					730:738	strengthened phenol oxidation	710:738	strengthened phenol oxidation by Fe3O4-induced hydroxyl radicals (·OH)	710:779	Introduction of nano-Fe3O4 to the composite gel further enhanced phenol degradation rate by >10% owing to strengthened phenol oxidation by Fe3O4-induced hydroxyl radicals (·OH) and improved mass and electron transfers.
34856163	1	13	theme	mechanical	214:223	arg1	strength					225:232	mechanical strength	214:232	mechanical strength	214:232	It's of practical importance but highly challenging for cell immobilization supports to maintain mechanical strength and reduce microbial leakage in environmental and industrial applications.
34856163	4	14	theme	high	585:588	arg1	loading					595:601	high cell loading	585:601	high cell loading	585:601	pneumoniae composite bead showed good pelletizing properties, superior material strength and high cell loading.
34856163	4	14	theme	high	585:588	arg1	properties					542:551	good pelletizing properties	525:551	good pelletizing properties	525:551	pneumoniae composite bead showed good pelletizing properties, superior material strength and high cell loading.
34856163	5	15	theme	phenol	723:728	arg1	oxidation					730:738	strengthened phenol oxidation	710:738	strengthened phenol oxidation by Fe3O4-induced hydroxyl radicals (·OH)	710:779	Introduction of nano-Fe3O4 to the composite gel further enhanced phenol degradation rate by >10% owing to strengthened phenol oxidation by Fe3O4-induced hydroxyl radicals (·OH) and improved mass and electron transfers.
34856163	5	16	theme	phenol	669:674	arg1	rate					688:691	phenol degradation rate	669:691	phenol degradation rate	669:691	Introduction of nano-Fe3O4 to the composite gel further enhanced phenol degradation rate by >10% owing to strengthened phenol oxidation by Fe3O4-induced hydroxyl radicals (·OH) and improved mass and electron transfers.
34856163	7	17	theme	15th	1050:1053	arg1	cycles					1055:1060	the 15th cycles	1046:1060	the 15th cycles	1046:1060	pneumoniae composite bead showed that 1500 mg/L phenol was fully degraded for all cycles with the highest rate of 55.12 mg L-1·h-1 obtained at the 15th cycles.
34856163	5	18	theme	degradation	676:686	arg1	rate					688:691	phenol degradation rate	669:691	phenol degradation rate	669:691	Introduction of nano-Fe3O4 to the composite gel further enhanced phenol degradation rate by >10% owing to strengthened phenol oxidation by Fe3O4-induced hydroxyl radicals (·OH) and improved mass and electron transfers.
34856163	2	19	theme	phenol	453:458	arg1	wastes					460:465	processing phenol wastes	442:465	processing phenol wastes	442:465	Herein, we developed an agar/κ-carrageenan composite hydrogel to entrap Klebsiella pneumoniae with the combination of nano-Fe3O4 for processing phenol wastes.
34856163	2	20	theme	processing	442:451	arg1	wastes					460:465	processing phenol wastes	442:465	processing phenol wastes	442:465	Herein, we developed an agar/κ-carrageenan composite hydrogel to entrap Klebsiella pneumoniae with the combination of nano-Fe3O4 for processing phenol wastes.
34856163	0	21	theme	agar/carrageenan-Fe3O4-Klebsiella	22:54	arg1	beads					77:81	stable agar/carrageenan-Fe3O4-Klebsiella pneumoniae composite beads	15:81	stable agar/carrageenan-Fe3O4-Klebsiella pneumoniae composite beads	15:81	Development of stable agar/carrageenan-Fe3O4-Klebsiella pneumoniae composite beads for efficient phenol degradation.
34856163	8	22	theme	phenol-degrading	1139:1154	arg1	bacteria					1156:1163	the as-prepared immobilized phenol-degrading bacteria	1111:1163	the as-prepared immobilized phenol-degrading bacteria	1111:1163	The improved stability and recyclability render the as-prepared immobilized phenol-degrading bacteria with great potential for industrial applications.
34856163	0	23	theme	stable	15:20	arg1	beads					77:81	stable agar/carrageenan-Fe3O4-Klebsiella pneumoniae composite beads	15:81	stable agar/carrageenan-Fe3O4-Klebsiella pneumoniae composite beads	15:81	Development of stable agar/carrageenan-Fe3O4-Klebsiella pneumoniae composite beads for efficient phenol degradation.
34856163	7	24	theme	pneumoniae	903:912	arg1	bead					924:927	pneumoniae composite bead	903:927	pneumoniae composite bead	903:927	pneumoniae composite bead showed that 1500 mg/L phenol was fully degraded for all cycles with the highest rate of 55.12 mg L-1·h-1 obtained at the 15th cycles.
34856163	1	25	theme	practical	125:133	arg1	importance					135:144	practical importance	125:144	practical importance	125:144	It's of practical importance but highly challenging for cell immobilization supports to maintain mechanical strength and reduce microbial leakage in environmental and industrial applications.
34856163	0	26	theme	composite	67:75	arg1	beads					77:81	stable agar/carrageenan-Fe3O4-Klebsiella pneumoniae composite beads	15:81	stable agar/carrageenan-Fe3O4-Klebsiella pneumoniae composite beads	15:81	Development of stable agar/carrageenan-Fe3O4-Klebsiella pneumoniae composite beads for efficient phenol degradation.
34856163	7	27	theme	composite	914:922	arg1	bead					924:927	pneumoniae composite bead	903:927	pneumoniae composite bead	903:927	pneumoniae composite bead showed that 1500 mg/L phenol was fully degraded for all cycles with the highest rate of 55.12 mg L-1·h-1 obtained at the 15th cycles.
34856163	5	28	theme	electron	803:810	arg1	transfers					812:820	electron transfers	803:820	electron transfers	803:820	Introduction of nano-Fe3O4 to the composite gel further enhanced phenol degradation rate by >10% owing to strengthened phenol oxidation by Fe3O4-induced hydroxyl radicals (·OH) and improved mass and electron transfers.
34856163	4	29	theme	pelletizing	530:540	arg1	properties					542:551	good pelletizing properties	525:551	good pelletizing properties	525:551	pneumoniae composite bead showed good pelletizing properties, superior material strength and high cell loading.
34856163	4	29	theme	pelletizing	530:540	arg1	strength					572:579	superior material strength	554:579	superior material strength	554:579	pneumoniae composite bead showed good pelletizing properties, superior material strength and high cell loading.
34856163	4	29	theme	pelletizing	530:540	arg1	loading					595:601	high cell loading	585:601	high cell loading	585:601	pneumoniae composite bead showed good pelletizing properties, superior material strength and high cell loading.
34856163	7	30	theme	1500 mg/L	941:949	arg1	phenol					951:956	1500 mg/L phenol	941:956	1500 mg/L phenol	941:956	pneumoniae composite bead showed that 1500 mg/L phenol was fully degraded for all cycles with the highest rate of 55.12 mg L-1·h-1 obtained at the 15th cycles.
34856163	1	31	theme	microbial	245:253	arg1	leakage					255:261	microbial leakage	245:261	microbial leakage in environmental and industrial applications	245:306	It's of practical importance but highly challenging for cell immobilization supports to maintain mechanical strength and reduce microbial leakage in environmental and industrial applications.
34856163	0	32	theme	pneumoniae	56:65	arg1	beads					77:81	stable agar/carrageenan-Fe3O4-Klebsiella pneumoniae composite beads	15:81	stable agar/carrageenan-Fe3O4-Klebsiella pneumoniae composite beads	15:81	Development of stable agar/carrageenan-Fe3O4-Klebsiella pneumoniae composite beads for efficient phenol degradation.
34856163	4	33	theme	superior	554:561	arg1	properties					542:551	good pelletizing properties	525:551	good pelletizing properties	525:551	pneumoniae composite bead showed good pelletizing properties, superior material strength and high cell loading.
34856163	4	33	theme	superior	554:561	arg1	strength					572:579	superior material strength	554:579	superior material strength	554:579	pneumoniae composite bead showed good pelletizing properties, superior material strength and high cell loading.
34856163	5	34	theme	composite	638:646	arg1	gel					648:650	the composite gel	634:650	the composite gel	634:650	Introduction of nano-Fe3O4 to the composite gel further enhanced phenol degradation rate by >10% owing to strengthened phenol oxidation by Fe3O4-induced hydroxyl radicals (·OH) and improved mass and electron transfers.
34856163	6	35	theme	successive	826:835	arg1	cycles					837:842	50 successive cycles	823:842	50 successive cycles of degradation and recycling	823:871	50 successive cycles of degradation and recycling using the agar/carrageenan-K.
34856163	1	36	from	leakage	255:261	arg1	applications					295:306	environmental and industrial applications	266:306	environmental and industrial applications	266:306	It's of practical importance but highly challenging for cell immobilization supports to maintain mechanical strength and reduce microbial leakage in environmental and industrial applications.
34856163	0	37	theme	beads	77:81	arg1	Development					0:10	Development	0:10	Development of stable agar/carrageenan-Fe3O4-Klebsiella pneumoniae composite beads for efficient phenol degradation.	0:115	Development of stable agar/carrageenan-Fe3O4-Klebsiella pneumoniae composite beads for efficient phenol degradation.
34856163	1	38	theme	cell	173:176	arg1	immobilization					178:191	cell immobilization	173:191	cell immobilization	173:191	It's of practical importance but highly challenging for cell immobilization supports to maintain mechanical strength and reduce microbial leakage in environmental and industrial applications.
34856163	6	39	theme	degradation	847:857	arg1	cycles					837:842	50 successive cycles	823:842	50 successive cycles of degradation and recycling	823:871	50 successive cycles of degradation and recycling using the agar/carrageenan-K.
34856163	8	40	theme	industrial	1190:1199	arg1	applications					1201:1212	industrial applications	1190:1212	industrial applications	1190:1212	The improved stability and recyclability render the as-prepared immobilized phenol-degrading bacteria with great potential for industrial applications.
34856163	7	41	theme	55.12 mg L-1·h-1	1017:1032	arg1	rate					1009:1012	the highest rate	997:1012	the highest rate of 55.12 mg L-1·h-1 obtained at the 15th cycles	997:1060	pneumoniae composite bead showed that 1500 mg/L phenol was fully degraded for all cycles with the highest rate of 55.12 mg L-1·h-1 obtained at the 15th cycles.
34856163	4	42	theme	composite	503:511	arg1	bead					513:516	pneumoniae composite bead	492:516	pneumoniae composite bead	492:516	pneumoniae composite bead showed good pelletizing properties, superior material strength and high cell loading.
34856163	4	43	theme	good	525:528	arg1	properties					542:551	good pelletizing properties	525:551	good pelletizing properties	525:551	pneumoniae composite bead showed good pelletizing properties, superior material strength and high cell loading.
34856163	4	43	theme	good	525:528	arg1	strength					572:579	superior material strength	554:579	superior material strength	554:579	pneumoniae composite bead showed good pelletizing properties, superior material strength and high cell loading.
34856163	4	43	theme	good	525:528	arg1	loading					595:601	high cell loading	585:601	high cell loading	585:601	pneumoniae composite bead showed good pelletizing properties, superior material strength and high cell loading.
34856163	6	44	theme	recycling	863:871	arg1	cycles					837:842	50 successive cycles	823:842	50 successive cycles of degradation and recycling	823:871	50 successive cycles of degradation and recycling using the agar/carrageenan-K.
34856163	2	45	theme	Klebsiella	381:390	arg1	pneumoniae					392:401	Klebsiella pneumoniae	381:401	Klebsiella pneumoniae	381:401	Herein, we developed an agar/κ-carrageenan composite hydrogel to entrap Klebsiella pneumoniae with the combination of nano-Fe3O4 for processing phenol wastes.
34856163	1	46	theme	environmental	266:278	arg1	applications					295:306	environmental and industrial applications	266:306	environmental and industrial applications	266:306	It's of practical importance but highly challenging for cell immobilization supports to maintain mechanical strength and reduce microbial leakage in environmental and industrial applications.
34856163	0	47	theme	phenol	97:102	arg1	degradation					104:114	efficient phenol degradation	87:114	efficient phenol degradation	87:114	Development of stable agar/carrageenan-Fe3O4-Klebsiella pneumoniae composite beads for efficient phenol degradation.
34856163	5	48	theme	nano-Fe3O4	620:629	arg1	Introduction					604:615	Introduction	604:615	Introduction of nano-Fe3O4 to the composite gel	604:650	Introduction of nano-Fe3O4 to the composite gel further enhanced phenol degradation rate by >10% owing to strengthened phenol oxidation by Fe3O4-induced hydroxyl radicals (·OH) and improved mass and electron transfers.
34856163	0	49	theme	efficient	87:95	arg1	degradation					104:114	efficient phenol degradation	87:114	efficient phenol degradation	87:114	Development of stable agar/carrageenan-Fe3O4-Klebsiella pneumoniae composite beads for efficient phenol degradation.
34856163	7	50	with	cycles	985:990	arg1	rate					1009:1012	the highest rate	997:1012	the highest rate of 55.12 mg L-1·h-1 obtained at the 15th cycles	997:1060	pneumoniae composite bead showed that 1500 mg/L phenol was fully degraded for all cycles with the highest rate of 55.12 mg L-1·h-1 obtained at the 15th cycles.
34856163	2	51	theme	nano-Fe3O4	427:436	arg1	combination					412:422	the combination	408:422	the combination of nano-Fe3O4 for processing phenol wastes	408:465	Herein, we developed an agar/κ-carrageenan composite hydrogel to entrap Klebsiella pneumoniae with the combination of nano-Fe3O4 for processing phenol wastes.
34856163	1	52	theme	industrial	284:293	arg1	applications					295:306	environmental and industrial applications	266:306	environmental and industrial applications	266:306	It's of practical importance but highly challenging for cell immobilization supports to maintain mechanical strength and reduce microbial leakage in environmental and industrial applications.
31679006	5	0	theme	dry	763:765	arg1	periods					767:773	seasonal dry periods	754:773	seasonal dry periods	754:773	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	11	1	link	β-3-linked	1736:1745	arg1	rhamnogalactofuranan					1747:1766	a β-3-linked rhamnogalactofuranan	1734:1766	a β-3-linked rhamnogalactofuranan	1734:1766	Exposure to D/R strongly altered the size distribution of TR9 hot-water-soluble polysaccharides, composed mainly of a β-3-linked rhamnogalactofuranan and Csol KOH-soluble β-glucans.
31679006	1	2	theme	most	175:178	arg1	features					192:199	the most distinctive features	171:199	the most distinctive features of desiccation-tolerant plants	171:230	BACKGROUND AND AIMS One of the most distinctive features of desiccation-tolerant plants is their high cell wall (CW) flexibility.
31679006	6	3	theme	Low-temperature	901:915	arg1	SEM					917:919	Low-temperature SEM	901:919	Low-temperature SEM	901:919	Low-temperature SEM, environmental SEM and freeze-substitution TEM were employed to visualize structural alterations induced by D/R.
31679006	10	4	theme	close	1582:1586	arg1	contact					1588:1594	close contact	1582:1594	close contact with the deformed CW	1582:1615	Notably, the plasma membrane of TR9 and Csol remained in close contact with the deformed CW.
31679006	1	5	theme	distinctive	180:190	arg1	features					192:199	the most distinctive features	171:199	the most distinctive features of desiccation-tolerant plants	171:230	BACKGROUND AND AIMS One of the most distinctive features of desiccation-tolerant plants is their high cell wall (CW) flexibility.
31679006	12	6	theme	Cyclic	1812:1817	arg1	D/R					1819:1821	Cyclic D/R	1812:1821	Cyclic D/R	1812:1821	CONCLUSIONS Cyclic D/R induces biochemical remodelling of the CW that could increase CW flexibility, allowing regulated shrinkage and expansion of D/R-tolerant microalgae.
31679006	0	7	theme	cyclic	112:117	arg1	desiccation-rehydration					119:141	cyclic desiccation-rehydration	112:141	cyclic desiccation-rehydration	112:141	Ultrastructural and biochemical analyses reveal cell wall remodelling in lichen-forming microalgae submitted to cyclic desiccation-rehydration.
31679006	11	8	theme	hot-water-soluble	1680:1696	arg1	polysaccharides					1698:1712	TR9 hot-water-soluble polysaccharides	1676:1712	TR9 hot-water-soluble polysaccharides	1676:1712	Exposure to D/R strongly altered the size distribution of TR9 hot-water-soluble polysaccharides, composed mainly of a β-3-linked rhamnogalactofuranan and Csol KOH-soluble β-glucans.
31679006	2	9	theme	drastic	310:316	arg1	D/R					343:345	D/R	343:345	D/R	343:345	Most lichen microalgae can tolerate drastic dehydration-rehydration (D/R) conditions; however, their mechanisms of D/R tolerance are scarcely understood.
31679006	2	9	theme	drastic	310:316	arg1	dehydration-rehydration					318:340	drastic dehydration-rehydration	310:340	drastic dehydration-rehydration (D/R) conditions	310:357	Most lichen microalgae can tolerate drastic dehydration-rehydration (D/R) conditions; however, their mechanisms of D/R tolerance are scarcely understood.
31679006	9	10	theme	ultrastructural	1326:1340	arg1	analyses					1342:1349	All ultrastructural analyses	1322:1349	All ultrastructural analyses	1322:1349	KEY RESULTS All ultrastructural analyses consistently showed that desiccation caused progressive cell shrinkage and deformation in both microalgae, which could be rapidly reversed when water availability increased.
31679006	9	11	theme	water	1495:1499	arg1	availability					1501:1512	water availability	1495:1512	water availability	1495:1512	KEY RESULTS All ultrastructural analyses consistently showed that desiccation caused progressive cell shrinkage and deformation in both microalgae, which could be rapidly reversed when water availability increased.
31679006	8	12	theme	main	1245:1248	arg1	polysaccharides					1250:1264	the main polysaccharides	1241:1264	the main polysaccharides of each CW fraction	1241:1284	The glycosyl composition and linkage of the main polysaccharides of each CW fraction were analysed by GC-MS.
31679006	5	13	theme	%	852:852	arg1	no					792:793	no	792:793	no	792:793	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	5	13	theme	%	852:852	arg1	cycles					803:808	four cycles	798:808	four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH)	798:898	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	12	14	theme	biochemical	1831:1841	arg1	remodelling					1843:1853	biochemical remodelling	1831:1853	biochemical remodelling of the CW that could increase CW flexibility	1831:1898	CONCLUSIONS Cyclic D/R induces biochemical remodelling of the CW that could increase CW flexibility, allowing regulated shrinkage and expansion of D/R-tolerant microalgae.
31679006	11	15	theme	polysaccharides	1698:1712	arg1	distribution					1660:1671	the size distribution	1651:1671	the size distribution of TR9 hot-water-soluble polysaccharides, composed mainly of a β-3-linked rhamnogalactofuranan and Csol KOH-soluble β-glucans	1651:1797	Exposure to D/R strongly altered the size distribution of TR9 hot-water-soluble polysaccharides, composed mainly of a β-3-linked rhamnogalactofuranan and Csol KOH-soluble β-glucans.
31679006	7	16	theme	polysaccharides	1154:1168	arg1	profile					1143:1149	the gel permeation profile	1124:1149	the gel permeation profile of polysaccharides	1124:1168	In addition, CWs were extracted and sequentially fractionated with hot water and KOH, and the gel permeation profile of polysaccharides was analysed in each fraction.
31679006	5	17	theme	desiccation	813:823	arg1	%					832:832	desiccation [25-30 % RH	813:835	desiccation [25-30 % RH (TR9)	813:841	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	5	17	theme	desiccation	813:823	arg1	TR9					838:840	TR9	838:840	TR9	838:840	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	5	17	theme	desiccation	813:823	arg1	rehydration					877:887	rehydration	877:887	rehydration	877:887	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	4	18	theme	Trebouxia	661:669	arg1	microalgae					649:658	microalgae	649:658	microalgae	649:658	METHODS Two lichen microalgae, Trebouxia sp.
31679006	4	18	theme	Trebouxia	661:669	arg1	sp					671:672	Trebouxia sp	661:672	Trebouxia sp	661:672	METHODS Two lichen microalgae, Trebouxia sp.
31679006	1	19	dep	BACKGROUND	144:153	arg1	One					164:166	One	164:166	One	164:166	BACKGROUND AND AIMS One of the most distinctive features of desiccation-tolerant plants is their high cell wall (CW) flexibility.
31679006	1	20	theme	features	192:199	arg1	AIMS					159:162	AIMS	159:162	AIMS	159:162	BACKGROUND AND AIMS One of the most distinctive features of desiccation-tolerant plants is their high cell wall (CW) flexibility.
31679006	1	20	theme	features	192:199	arg1	BACKGROUND					144:153	BACKGROUND	144:153	BACKGROUND	144:153	BACKGROUND AND AIMS One of the most distinctive features of desiccation-tolerant plants is their high cell wall (CW) flexibility.
31679006	12	21	theme	regulated	1910:1918	arg1	shrinkage					1920:1928	regulated shrinkage	1910:1928	regulated shrinkage	1910:1928	CONCLUSIONS Cyclic D/R induces biochemical remodelling of the CW that could increase CW flexibility, allowing regulated shrinkage and expansion of D/R-tolerant microalgae.
31679006	7	22	theme	hot	1101:1103	arg1	water					1105:1109	hot water	1101:1109	hot water	1101:1109	In addition, CWs were extracted and sequentially fractionated with hot water and KOH, and the gel permeation profile of polysaccharides was analysed in each fraction.
31679006	12	23	theme	CW	1885:1886	arg1	flexibility					1888:1898	CW flexibility	1885:1898	CW flexibility	1885:1898	CONCLUSIONS Cyclic D/R induces biochemical remodelling of the CW that could increase CW flexibility, allowing regulated shrinkage and expansion of D/R-tolerant microalgae.
31679006	10	24	theme	plasma	1538:1543	arg1	membrane					1545:1552	the plasma membrane	1534:1552	the plasma membrane of TR9 and Csol	1534:1568	Notably, the plasma membrane of TR9 and Csol remained in close contact with the deformed CW.
31679006	6	25	theme	structural	995:1004	arg1	alterations					1006:1016	structural alterations	995:1016	structural alterations induced by D/R	995:1031	Low-temperature SEM, environmental SEM and freeze-substitution TEM were employed to visualize structural alterations induced by D/R.
31679006	7	26	theme	gel	1128:1130	arg1	profile					1143:1149	the gel permeation profile	1124:1149	the gel permeation profile of polysaccharides	1124:1168	In addition, CWs were extracted and sequentially fractionated with hot water and KOH, and the gel permeation profile of polysaccharides was analysed in each fraction.
31679006	8	27	theme	fraction	1277:1284	arg1	polysaccharides					1250:1264	the main polysaccharides	1241:1264	the main polysaccharides of each CW fraction	1241:1284	The glycosyl composition and linkage of the main polysaccharides of each CW fraction were analysed by GC-MS.
31679006	10	28	with	contact	1588:1594	arg1	CW					1614:1615	the deformed CW	1601:1615	the deformed CW	1601:1615	Notably, the plasma membrane of TR9 and Csol remained in close contact with the deformed CW.
31679006	1	29	theme	desiccation-tolerant	204:223	arg1	plants					225:230	desiccation-tolerant plants	204:230	desiccation-tolerant plants	204:230	BACKGROUND AND AIMS One of the most distinctive features of desiccation-tolerant plants is their high cell wall (CW) flexibility.
31679006	0	30	theme	Ultrastructural	0:14	arg1	analyses					32:39	Ultrastructural and biochemical analyses	0:39	Ultrastructural and biochemical analyses	0:39	Ultrastructural and biochemical analyses reveal cell wall remodelling in lichen-forming microalgae submitted to cyclic desiccation-rehydration.
31679006	11	31	theme	TR9	1676:1678	arg1	polysaccharides					1698:1712	TR9 hot-water-soluble polysaccharides	1676:1712	TR9 hot-water-soluble polysaccharides	1676:1712	Exposure to D/R strongly altered the size distribution of TR9 hot-water-soluble polysaccharides, composed mainly of a β-3-linked rhamnogalactofuranan and Csol KOH-soluble β-glucans.
31679006	12	32	theme	CW	1862:1863	arg1	remodelling					1843:1853	biochemical remodelling	1831:1853	biochemical remodelling of the CW that could increase CW flexibility	1831:1898	CONCLUSIONS Cyclic D/R induces biochemical remodelling of the CW that could increase CW flexibility, allowing regulated shrinkage and expansion of D/R-tolerant microalgae.
31679006	8	33	theme	CW	1274:1275	arg1	fraction					1277:1284	each CW fraction	1269:1284	each CW fraction	1269:1284	The glycosyl composition and linkage of the main polysaccharides of each CW fraction were analysed by GC-MS.
31679006	2	34	theme	tolerance	393:401	arg1	mechanisms					375:384	their mechanisms	369:384	their mechanisms of D/R tolerance	369:401	Most lichen microalgae can tolerate drastic dehydration-rehydration (D/R) conditions; however, their mechanisms of D/R tolerance are scarcely understood.
31679006	12	35	theme	D/R-tolerant	1947:1958	arg1	microalgae					1960:1969	D/R-tolerant microalgae	1947:1969	D/R-tolerant microalgae	1947:1969	CONCLUSIONS Cyclic D/R induces biochemical remodelling of the CW that could increase CW flexibility, allowing regulated shrinkage and expansion of D/R-tolerant microalgae.
31679006	11	36	theme	β-3-linked	1736:1745	arg1	rhamnogalactofuranan					1747:1766	a β-3-linked rhamnogalactofuranan	1734:1766	a β-3-linked rhamnogalactofuranan	1734:1766	Exposure to D/R strongly altered the size distribution of TR9 hot-water-soluble polysaccharides, composed mainly of a β-3-linked rhamnogalactofuranan and Csol KOH-soluble β-glucans.
31679006	9	37	from	deformation	1426:1436	arg1	microalgae					1446:1455	both microalgae	1441:1455	both microalgae	1441:1455	KEY RESULTS All ultrastructural analyses consistently showed that desiccation caused progressive cell shrinkage and deformation in both microalgae, which could be rapidly reversed when water availability increased.
31679006	11	38	theme	KOH-soluble	1777:1787	arg1	β-glucans					1789:1797	Csol KOH-soluble β-glucans	1772:1797	Csol KOH-soluble β-glucans	1772:1797	Exposure to D/R strongly altered the size distribution of TR9 hot-water-soluble polysaccharides, composed mainly of a β-3-linked rhamnogalactofuranan and Csol KOH-soluble β-glucans.
31679006	3	39	theme	flexible	493:500	arg1	CWs					502:504	flexible CWs	493:504	flexible CWs	493:504	We tested the hypothesis that D/R-tolerant microalgae would have flexible CWs due to species-specific CW ultrastructure and biochemical composition, which could be remodelled by exposure to cyclic D/R.
31679006	0	40	theme	biochemical	20:30	arg1	analyses					32:39	Ultrastructural and biochemical analyses	0:39	Ultrastructural and biochemical analyses	0:39	Ultrastructural and biochemical analyses reveal cell wall remodelling in lichen-forming microalgae submitted to cyclic desiccation-rehydration.
31679006	9	41	theme	cell	1407:1410	arg1	shrinkage					1412:1420	progressive cell shrinkage	1395:1420	progressive cell shrinkage	1395:1420	KEY RESULTS All ultrastructural analyses consistently showed that desiccation caused progressive cell shrinkage and deformation in both microalgae, which could be rapidly reversed when water availability increased.
31679006	3	42	contain	have	488:491	arg2	CWs					502:504	flexible CWs	493:504	flexible CWs	493:504	We tested the hypothesis that D/R-tolerant microalgae would have flexible CWs due to species-specific CW ultrastructure and biochemical composition, which could be remodelled by exposure to cyclic D/R.
31679006	3	42	contain	have	488:491	arg1	microalgae					471:480	D/R-tolerant microalgae	458:480	D/R-tolerant microalgae	458:480	We tested the hypothesis that D/R-tolerant microalgae would have flexible CWs due to species-specific CW ultrastructure and biochemical composition, which could be remodelled by exposure to cyclic D/R.
31679006	2	43	theme	dehydration-rehydration	318:340	arg1	conditions					348:357	drastic dehydration-rehydration (D/R) conditions	310:357	drastic dehydration-rehydration (D/R) conditions	310:357	Most lichen microalgae can tolerate drastic dehydration-rehydration (D/R) conditions; however, their mechanisms of D/R tolerance are scarcely understood.
31679006	12	44	theme	microalgae	1960:1969	arg1	expansion					1934:1942	expansion	1934:1942	expansion	1934:1942	CONCLUSIONS Cyclic D/R induces biochemical remodelling of the CW that could increase CW flexibility, allowing regulated shrinkage and expansion of D/R-tolerant microalgae.
31679006	12	44	theme	microalgae	1960:1969	arg1	shrinkage					1920:1928	regulated shrinkage	1910:1928	regulated shrinkage	1910:1928	CONCLUSIONS Cyclic D/R induces biochemical remodelling of the CW that could increase CW flexibility, allowing regulated shrinkage and expansion of D/R-tolerant microalgae.
31679006	3	45	theme	biochemical	552:562	arg1	composition					564:574	biochemical composition	552:574	biochemical composition	552:574	We tested the hypothesis that D/R-tolerant microalgae would have flexible CWs due to species-specific CW ultrastructure and biochemical composition, which could be remodelled by exposure to cyclic D/R.
31679006	0	46	theme	cell	48:51	arg1	remodelling					58:68	cell wall remodelling	48:68	cell wall remodelling	48:68	Ultrastructural and biochemical analyses reveal cell wall remodelling in lichen-forming microalgae submitted to cyclic desiccation-rehydration.
31679006	5	47	theme	rehydration	877:887	arg1	Csol					858:861	Csol	858:861	Csol	858:861	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	5	47	theme	rehydration	877:887	arg1	rehydration					877:887	rehydration	877:887	rehydration	877:887	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	5	47	theme	rehydration	877:887	arg1	%					832:832	desiccation [25-30 % RH	813:835	desiccation [25-30 % RH (TR9)	813:841	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	5	47	theme	rehydration	877:887	arg1	%					894:894	100 % RH	890:897	100 % RH	890:897	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	5	47	theme	rehydration	877:887	arg1	%					852:852	55-60 % RH	846:855	55-60 % RH (Csol)]	846:863	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	5	47	theme	rehydration	877:887	arg1	h					872:872	16 h	869:872	16 h of rehydration	869:887	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	5	47	theme	rehydration	877:887	arg1	TR9					838:840	TR9	838:840	TR9	838:840	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	5	48	theme	Coccomyxa	718:726	arg1	simplex					728:734	Coccomyxa simplex	718:734	Coccomyxa simplex (Csol, adapted to seasonal dry periods)	718:774	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	6	49	theme	freeze-substitution	944:962	arg1	TEM					964:966	freeze-substitution TEM	944:966	freeze-substitution TEM	944:966	Low-temperature SEM, environmental SEM and freeze-substitution TEM were employed to visualize structural alterations induced by D/R.
31679006	1	50	theme	plants	225:230	arg1	features					192:199	the most distinctive features	171:199	the most distinctive features of desiccation-tolerant plants	171:230	BACKGROUND AND AIMS One of the most distinctive features of desiccation-tolerant plants is their high cell wall (CW) flexibility.
31679006	1	51	theme	high	241:244	arg1	flexibility					261:271	their high cell wall (CW) flexibility	235:271	their high cell wall (CW) flexibility	235:271	BACKGROUND AND AIMS One of the most distinctive features of desiccation-tolerant plants is their high cell wall (CW) flexibility.
31679006	3	52	theme	D/R-tolerant	458:469	arg1	microalgae					471:480	D/R-tolerant microalgae	458:480	D/R-tolerant microalgae	458:480	We tested the hypothesis that D/R-tolerant microalgae would have flexible CWs due to species-specific CW ultrastructure and biochemical composition, which could be remodelled by exposure to cyclic D/R.
31679006	11	53	theme	Csol	1772:1775	arg1	β-glucans					1789:1797	Csol KOH-soluble β-glucans	1772:1797	Csol KOH-soluble β-glucans	1772:1797	Exposure to D/R strongly altered the size distribution of TR9 hot-water-soluble polysaccharides, composed mainly of a β-3-linked rhamnogalactofuranan and Csol KOH-soluble β-glucans.
31679006	5	54	dep	TR9	675:677	arg1	TR9					680:682	TR9	680:682	TR9	680:682	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	11	55	theme	size	1655:1658	arg1	distribution					1660:1671	the size distribution	1651:1671	the size distribution of TR9 hot-water-soluble polysaccharides, composed mainly of a β-3-linked rhamnogalactofuranan and Csol KOH-soluble β-glucans	1651:1797	Exposure to D/R strongly altered the size distribution of TR9 hot-water-soluble polysaccharides, composed mainly of a β-3-linked rhamnogalactofuranan and Csol KOH-soluble β-glucans.
31679006	1	56	theme	cell	246:249	arg1	flexibility					261:271	their high cell wall (CW) flexibility	235:271	their high cell wall (CW) flexibility	235:271	BACKGROUND AND AIMS One of the most distinctive features of desiccation-tolerant plants is their high cell wall (CW) flexibility.
31679006	10	57	theme	Csol	1565:1568	arg1	membrane					1545:1552	the plasma membrane	1534:1552	the plasma membrane of TR9 and Csol	1534:1568	Notably, the plasma membrane of TR9 and Csol remained in close contact with the deformed CW.
31679006	8	58	theme	polysaccharides	1250:1264	arg1	linkage					1230:1236	linkage	1230:1236	linkage	1230:1236	The glycosyl composition and linkage of the main polysaccharides of each CW fraction were analysed by GC-MS.
31679006	8	58	theme	polysaccharides	1250:1264	arg1	composition					1214:1224	composition	1214:1224	composition	1214:1224	The glycosyl composition and linkage of the main polysaccharides of each CW fraction were analysed by GC-MS.
31679006	5	59	dep	simplex	728:734	arg1	Csol					737:740	Csol	737:740	Csol	737:740	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	3	60	theme	cyclic	618:623	arg1	D/R					625:627	cyclic D/R	618:627	cyclic D/R	618:627	We tested the hypothesis that D/R-tolerant microalgae would have flexible CWs due to species-specific CW ultrastructure and biochemical composition, which could be remodelled by exposure to cyclic D/R.
31679006	9	61	from	shrinkage	1412:1420	arg1	microalgae					1446:1455	both microalgae	1441:1455	both microalgae	1441:1455	KEY RESULTS All ultrastructural analyses consistently showed that desiccation caused progressive cell shrinkage and deformation in both microalgae, which could be rapidly reversed when water availability increased.
31679006	3	62	theme	species-specific	513:528	arg1	ultrastructure					533:546	species-specific CW ultrastructure	513:546	species-specific CW ultrastructure	513:546	We tested the hypothesis that D/R-tolerant microalgae would have flexible CWs due to species-specific CW ultrastructure and biochemical composition, which could be remodelled by exposure to cyclic D/R.
31679006	0	63	theme	wall	53:56	arg1	remodelling					58:68	cell wall remodelling	48:68	cell wall remodelling	48:68	Ultrastructural and biochemical analyses reveal cell wall remodelling in lichen-forming microalgae submitted to cyclic desiccation-rehydration.
31679006	5	64	theme	rapid	696:700	arg1	cycles					706:711	rapid D/R cycles	696:711	rapid D/R cycles	696:711	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	9	65	theme	progressive	1395:1405	arg1	shrinkage					1412:1420	progressive cell shrinkage	1395:1420	progressive cell shrinkage	1395:1420	KEY RESULTS All ultrastructural analyses consistently showed that desiccation caused progressive cell shrinkage and deformation in both microalgae, which could be rapidly reversed when water availability increased.
31679006	3	66	theme	CW	530:531	arg1	ultrastructure					533:546	species-specific CW ultrastructure	513:546	species-specific CW ultrastructure	513:546	We tested the hypothesis that D/R-tolerant microalgae would have flexible CWs due to species-specific CW ultrastructure and biochemical composition, which could be remodelled by exposure to cyclic D/R.
31679006	5	67	theme	D/R	702:704	arg1	cycles					706:711	rapid D/R cycles	696:711	rapid D/R cycles	696:711	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	2	68	theme	lichen	279:284	arg1	microalgae					286:295	Most lichen microalgae	274:295	Most lichen microalgae	274:295	Most lichen microalgae can tolerate drastic dehydration-rehydration (D/R) conditions; however, their mechanisms of D/R tolerance are scarcely understood.
31679006	11	69	theme	β-glucans	1789:1797	arg1	distribution					1660:1671	the size distribution	1651:1671	the size distribution of TR9 hot-water-soluble polysaccharides, composed mainly of a β-3-linked rhamnogalactofuranan and Csol KOH-soluble β-glucans	1651:1797	Exposure to D/R strongly altered the size distribution of TR9 hot-water-soluble polysaccharides, composed mainly of a β-3-linked rhamnogalactofuranan and Csol KOH-soluble β-glucans.
31679006	1	70	theme	wall	251:254	arg1	flexibility					261:271	their high cell wall (CW) flexibility	235:271	their high cell wall (CW) flexibility	235:271	BACKGROUND AND AIMS One of the most distinctive features of desiccation-tolerant plants is their high cell wall (CW) flexibility.
31679006	9	71	dep	RESULTS	1314:1320	arg1	showed					1364:1369	showed	1364:1369	showed that desiccation caused progressive cell shrinkage and deformation in both microalgae, which could be rapidly reversed when water availability increased	1364:1522	KEY RESULTS All ultrastructural analyses consistently showed that desiccation caused progressive cell shrinkage and deformation in both microalgae, which could be rapidly reversed when water availability increased.
31679006	2	72	theme	Most	274:277	arg1	microalgae					286:295	Most lichen microalgae	274:295	Most lichen microalgae	274:295	Most lichen microalgae can tolerate drastic dehydration-rehydration (D/R) conditions; however, their mechanisms of D/R tolerance are scarcely understood.
31679006	8	73	theme	glycosyl	1205:1212	arg1	composition					1214:1224	composition	1214:1224	composition	1214:1224	The glycosyl composition and linkage of the main polysaccharides of each CW fraction were analysed by GC-MS.
31679006	6	74	theme	environmental	922:934	arg1	SEM					936:938	environmental SEM	922:938	environmental SEM	922:938	Low-temperature SEM, environmental SEM and freeze-substitution TEM were employed to visualize structural alterations induced by D/R.
31679006	0	75	theme	lichen-forming	73:86	arg1	microalgae					88:97	lichen-forming microalgae	73:97	lichen-forming microalgae submitted to cyclic desiccation-rehydration	73:141	Ultrastructural and biochemical analyses reveal cell wall remodelling in lichen-forming microalgae submitted to cyclic desiccation-rehydration.
31679006	5	76	theme	%	832:832	arg1	no					792:793	no	792:793	no	792:793	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	5	76	theme	%	832:832	arg1	cycles					803:808	four cycles	798:808	four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH)	798:898	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	12	77	dep	CONCLUSIONS	1800:1810	arg1	induces					1823:1829	induces	1823:1829	induces	1823:1829	CONCLUSIONS Cyclic D/R induces biochemical remodelling of the CW that could increase CW flexibility, allowing regulated shrinkage and expansion of D/R-tolerant microalgae.
31679006	10	78	theme	deformed	1605:1612	arg1	CW					1614:1615	the deformed CW	1601:1615	the deformed CW	1601:1615	Notably, the plasma membrane of TR9 and Csol remained in close contact with the deformed CW.
31679006	2	79	theme	D/R	389:391	arg1	tolerance					393:401	D/R tolerance	389:401	D/R tolerance	389:401	Most lichen microalgae can tolerate drastic dehydration-rehydration (D/R) conditions; however, their mechanisms of D/R tolerance are scarcely understood.
31679006	7	80	theme	permeation	1132:1141	arg1	profile					1143:1149	the gel permeation profile	1124:1149	the gel permeation profile of polysaccharides	1124:1168	In addition, CWs were extracted and sequentially fractionated with hot water and KOH, and the gel permeation profile of polysaccharides was analysed in each fraction.
31679006	1	81	theme	CW	257:258	arg1	flexibility					261:271	their high cell wall (CW) flexibility	235:271	their high cell wall (CW) flexibility	235:271	BACKGROUND AND AIMS One of the most distinctive features of desiccation-tolerant plants is their high cell wall (CW) flexibility.
31679006	5	82	theme	seasonal	754:761	arg1	periods					767:773	seasonal dry periods	754:773	seasonal dry periods	754:773	TR9 (TR9, adapted to rapid D/R cycles) and Coccomyxa simplex (Csol, adapted to seasonal dry periods) were exposed to no or four cycles of desiccation [25-30 % RH (TR9) or 55-60 % RH (Csol)] and 16 h of rehydration (100 % RH).
31679006	10	83	theme	TR9	1557:1559	arg1	membrane					1545:1552	the plasma membrane	1534:1552	the plasma membrane of TR9 and Csol	1534:1568	Notably, the plasma membrane of TR9 and Csol remained in close contact with the deformed CW.
31679006	9	84	theme	KEY	1310:1312	arg1	RESULTS					1314:1320	KEY RESULTS	1310:1320	KEY RESULTS All ultrastructural analyses consistently showed that desiccation caused progressive cell shrinkage and deformation in both microalgae, which could be rapidly reversed when water availability increased.	1310:1523	KEY RESULTS All ultrastructural analyses consistently showed that desiccation caused progressive cell shrinkage and deformation in both microalgae, which could be rapidly reversed when water availability increased.
34666134	2	0	theme	gums	527:530	arg1	properties					513:522	dynamic rheological and thermal properties	481:522	dynamic rheological and thermal properties of gums isolated from brown	481:550	The osidic and proximate composition, structure conformation, flow behaviour, dynamic rheological and thermal properties of gums isolated from brown and golden flaxseeds were assessed.
34666134	2	0	theme	gums	527:530	arg1	composition					428:438	composition	428:438	composition	428:438	The osidic and proximate composition, structure conformation, flow behaviour, dynamic rheological and thermal properties of gums isolated from brown and golden flaxseeds were assessed.
34666134	2	0	theme	gums	527:530	arg1	conformation					451:462	structure conformation	441:462	structure conformation	441:462	The osidic and proximate composition, structure conformation, flow behaviour, dynamic rheological and thermal properties of gums isolated from brown and golden flaxseeds were assessed.
34666134	2	0	theme	gums	527:530	arg1	behaviour					470:478	flow behaviour	465:478	flow behaviour	465:478	The osidic and proximate composition, structure conformation, flow behaviour, dynamic rheological and thermal properties of gums isolated from brown and golden flaxseeds were assessed.
34666134	2	0	theme	gums	527:530	arg1	flaxseeds					563:571	golden flaxseeds	556:571	golden flaxseeds	556:571	The osidic and proximate composition, structure conformation, flow behaviour, dynamic rheological and thermal properties of gums isolated from brown and golden flaxseeds were assessed.
34666134	6	1	theme	better	1328:1333	arg1	thickening					1335:1344	better thickening	1328:1344	better thickening	1328:1344	Golden flaxseed gums exerted higher molecular weight (Mw = 1.34-1.15 × 106 Da) and intrinsic viscosities (6.63-5.13 dL g-1) as well as better thickening and viscoelastic performance than the brown flaxseed gum exemplars.
34666134	3	2	from	changes	745:751	arg1	composition					766:776	the sugar composition	756:776	the sugar composition of the flaxseed gum extracts	756:805	Gum extraction under near-to-isoelectric point conditions did not impair the extraction yield, residual protein and ash content, whilst it resulted in minor changes in the sugar composition of the flaxseed gum extracts.
34666134	3	3	theme	extraction	665:674	arg1	yield					676:680	the extraction yield	661:680	the extraction yield	661:680	Gum extraction under near-to-isoelectric point conditions did not impair the extraction yield, residual protein and ash content, whilst it resulted in minor changes in the sugar composition of the flaxseed gum extracts.
34666134	3	4	theme	sugar	760:764	arg1	composition					766:776	the sugar composition	756:776	the sugar composition of the flaxseed gum extracts	756:805	Gum extraction under near-to-isoelectric point conditions did not impair the extraction yield, residual protein and ash content, whilst it resulted in minor changes in the sugar composition of the flaxseed gum extracts.
34666134	1	5	theme	mediated	255:262	arg1	swelling					268:275	gum swelling	264:275	gum swelling	264:275	The present work aimed at investigating an extraction protocol based on consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation as an alternative method to produce flaxseed gum extracts of enhanced techno-functional characteristics.
34666134	7	6	theme	severe	1584:1589	arg1	treatments					1596:1605	severe heat treatments	1584:1605	severe heat treatments	1584:1605	Golden flaxseed gums exhibited a better thermal stability compared to the brown flaxseed counterparts and therefore, they are suitable for product applications involving severe heat treatments.
34666134	5	7	theme	better	1134:1139	arg1	affinity					1151:1158	a better solvation affinity	1132:1158	a better solvation affinity in water and lyotropic solvents	1132:1190	The latter appeared to minimise the intra- and interchain polymer non-covalent interactions (hydrogen bonding) leading to a better solvation affinity in water and lyotropic solvents.
34666134	1	8	theme	gum	264:266	arg1	swelling					268:275	gum swelling	264:275	gum swelling	264:275	The present work aimed at investigating an extraction protocol based on consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation as an alternative method to produce flaxseed gum extracts of enhanced techno-functional characteristics.
34666134	5	9	theme	lyotropic	1173:1181	arg1	solvents					1183:1190	lyotropic solvents	1173:1190	lyotropic solvents	1173:1190	The latter appeared to minimise the intra- and interchain polymer non-covalent interactions (hydrogen bonding) leading to a better solvation affinity in water and lyotropic solvents.
34666134	1	10	theme	enhanced	359:366	arg1	characteristics					386:400	enhanced techno-functional characteristics	359:400	enhanced techno-functional characteristics	359:400	The present work aimed at investigating an extraction protocol based on consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation as an alternative method to produce flaxseed gum extracts of enhanced techno-functional characteristics.
34666134	1	11	theme	swelling	268:275	arg1	steps					216:220	consecutive steps	204:220	consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation	204:296	The present work aimed at investigating an extraction protocol based on consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation as an alternative method to produce flaxseed gum extracts of enhanced techno-functional characteristics.
34666134	0	12	theme	alkaline	100:107	arg1	conditions					120:129	alkaline and acidic conditions	100:129	conditions	120:129	Structure conformation, physicochemical and rheological properties of flaxseed gums extracted under alkaline and acidic conditions.
34666134	1	13	theme	techno-functional	368:384	arg1	characteristics					386:400	enhanced techno-functional characteristics	359:400	enhanced techno-functional characteristics	359:400	The present work aimed at investigating an extraction protocol based on consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation as an alternative method to produce flaxseed gum extracts of enhanced techno-functional characteristics.
34666134	5	14	theme	intra-	1046:1051	arg1	interactions					1089:1100	the intra- and interchain polymer non-covalent interactions	1042:1100	the intra- and interchain polymer non-covalent interactions (hydrogen bonding) leading to a better solvation affinity in water and lyotropic solvents	1042:1190	The latter appeared to minimise the intra- and interchain polymer non-covalent interactions (hydrogen bonding) leading to a better solvation affinity in water and lyotropic solvents.
34666134	5	14	theme	intra-	1046:1051	arg1	bonding					1112:1118	hydrogen bonding	1103:1118	hydrogen bonding	1103:1118	The latter appeared to minimise the intra- and interchain polymer non-covalent interactions (hydrogen bonding) leading to a better solvation affinity in water and lyotropic solvents.
34666134	3	15	theme	Gum	588:590	arg1	extraction					592:601	Gum extraction	588:601	Gum extraction under near-to-isoelectric point conditions	588:644	Gum extraction under near-to-isoelectric point conditions did not impair the extraction yield, residual protein and ash content, whilst it resulted in minor changes in the sugar composition of the flaxseed gum extracts.
34666134	5	16	from	affinity	1151:1158	arg1	water					1163:1167	water	1163:1167	water	1163:1167	The latter appeared to minimise the intra- and interchain polymer non-covalent interactions (hydrogen bonding) leading to a better solvation affinity in water and lyotropic solvents.
34666134	5	16	from	affinity	1151:1158	arg1	solvents					1183:1190	lyotropic solvents	1173:1190	lyotropic solvents	1173:1190	The latter appeared to minimise the intra- and interchain polymer non-covalent interactions (hydrogen bonding) leading to a better solvation affinity in water and lyotropic solvents.
34666134	1	17	theme	characteristics	386:400	arg1	extracts					347:354	flaxseed gum extracts	334:354	flaxseed gum extracts of enhanced techno-functional characteristics	334:400	The present work aimed at investigating an extraction protocol based on consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation as an alternative method to produce flaxseed gum extracts of enhanced techno-functional characteristics.
34666134	4	18	theme	major	886:890	arg1	populations					908:918	four major polysaccharidic populations	881:918	four major polysaccharidic populations corresponding to arabinoxylans, rhamnogalacturonan-I and two AX-RG-I composite fractions	881:1007	The deconvolution of the GPC/SEC chromatographs revealed the presence of four major polysaccharidic populations corresponding to arabinoxylans, rhamnogalacturonan-I and two AX-RG-I composite fractions.
34666134	1	19	theme	extraction	175:184	arg1	protocol					186:193	an extraction protocol	172:193	an extraction protocol based on consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation	172:296	The present work aimed at investigating an extraction protocol based on consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation as an alternative method to produce flaxseed gum extracts of enhanced techno-functional characteristics.
34666134	1	19	theme	extraction	175:184	arg1	method					316:321	an alternative method	301:321	an alternative method to produce flaxseed gum extracts of enhanced techno-functional characteristics	301:400	The present work aimed at investigating an extraction protocol based on consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation as an alternative method to produce flaxseed gum extracts of enhanced techno-functional characteristics.
34666134	0	20	theme	acidic	113:118	arg1	conditions					120:129	alkaline and acidic conditions	100:129	conditions	120:129	Structure conformation, physicochemical and rheological properties of flaxseed gums extracted under alkaline and acidic conditions.
34666134	5	21	theme	interchain	1057:1066	arg1	polymer					1068:1074	interchain polymer	1057:1074	interchain polymer	1057:1074	The latter appeared to minimise the intra- and interchain polymer non-covalent interactions (hydrogen bonding) leading to a better solvation affinity in water and lyotropic solvents.
34666134	7	22	theme	product	1553:1559	arg1	applications					1561:1572	product applications	1553:1572	product applications involving severe heat treatments	1553:1605	Golden flaxseed gums exhibited a better thermal stability compared to the brown flaxseed counterparts and therefore, they are suitable for product applications involving severe heat treatments.
34666134	2	23	theme	proximate	418:426	arg1	composition					428:438	composition	428:438	composition	428:438	The osidic and proximate composition, structure conformation, flow behaviour, dynamic rheological and thermal properties of gums isolated from brown and golden flaxseeds were assessed.
34666134	2	24	theme	dynamic	481:487	arg1	properties					513:522	dynamic rheological and thermal properties	481:522	dynamic rheological and thermal properties of gums isolated from brown	481:550	The osidic and proximate composition, structure conformation, flow behaviour, dynamic rheological and thermal properties of gums isolated from brown and golden flaxseeds were assessed.
34666134	6	25	theme	brown	1384:1388	arg1	exemplars					1403:1411	the brown flaxseed gum exemplars	1380:1411	the brown flaxseed gum exemplars	1380:1411	Golden flaxseed gums exerted higher molecular weight (Mw = 1.34-1.15 × 106 Da) and intrinsic viscosities (6.63-5.13 dL g-1) as well as better thickening and viscoelastic performance than the brown flaxseed gum exemplars.
34666134	4	26	theme	populations	908:918	arg1	presence					869:876	the presence	865:876	the presence of four major polysaccharidic populations corresponding to arabinoxylans, rhamnogalacturonan-I and two AX-RG-I composite fractions	865:1007	The deconvolution of the GPC/SEC chromatographs revealed the presence of four major polysaccharidic populations corresponding to arabinoxylans, rhamnogalacturonan-I and two AX-RG-I composite fractions.
34666134	3	27	theme	flaxseed	785:792	arg1	extracts					798:805	the flaxseed gum extracts	781:805	the flaxseed gum extracts	781:805	Gum extraction under near-to-isoelectric point conditions did not impair the extraction yield, residual protein and ash content, whilst it resulted in minor changes in the sugar composition of the flaxseed gum extracts.
34666134	3	28	theme	near-to-isoelectric	609:627	arg1	conditions					635:644	near-to-isoelectric point conditions	609:644	near-to-isoelectric point conditions	609:644	Gum extraction under near-to-isoelectric point conditions did not impair the extraction yield, residual protein and ash content, whilst it resulted in minor changes in the sugar composition of the flaxseed gum extracts.
34666134	7	29	theme	Golden	1414:1419	arg1	gums					1430:1433	Golden flaxseed gums	1414:1433	Golden flaxseed gums	1414:1433	Golden flaxseed gums exhibited a better thermal stability compared to the brown flaxseed counterparts and therefore, they are suitable for product applications involving severe heat treatments.
34666134	6	30	theme	6.63-5.13	1299:1307	arg1	g-1					1312:1314	6.63-5.13 dL g-1	1299:1314	6.63-5.13 dL g-1	1299:1314	Golden flaxseed gums exerted higher molecular weight (Mw = 1.34-1.15 × 106 Da) and intrinsic viscosities (6.63-5.13 dL g-1) as well as better thickening and viscoelastic performance than the brown flaxseed gum exemplars.
34666134	6	30	theme	6.63-5.13	1299:1307	arg1	viscosities					1286:1296	intrinsic viscosities	1276:1296	intrinsic viscosities (6.63-5.13 dL g-1)	1276:1315	Golden flaxseed gums exerted higher molecular weight (Mw = 1.34-1.15 × 106 Da) and intrinsic viscosities (6.63-5.13 dL g-1) as well as better thickening and viscoelastic performance than the brown flaxseed gum exemplars.
34666134	1	31	theme	deproteinisation	281:296	arg1	steps					216:220	consecutive steps	204:220	consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation	204:296	The present work aimed at investigating an extraction protocol based on consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation as an alternative method to produce flaxseed gum extracts of enhanced techno-functional characteristics.
34666134	4	32	theme	polysaccharidic	892:906	arg1	populations					908:918	four major polysaccharidic populations	881:918	four major polysaccharidic populations corresponding to arabinoxylans, rhamnogalacturonan-I and two AX-RG-I composite fractions	881:1007	The deconvolution of the GPC/SEC chromatographs revealed the presence of four major polysaccharidic populations corresponding to arabinoxylans, rhamnogalacturonan-I and two AX-RG-I composite fractions.
34666134	3	33	theme	point	629:633	arg1	conditions					635:644	near-to-isoelectric point conditions	609:644	near-to-isoelectric point conditions	609:644	Gum extraction under near-to-isoelectric point conditions did not impair the extraction yield, residual protein and ash content, whilst it resulted in minor changes in the sugar composition of the flaxseed gum extracts.
34666134	2	34	theme	osidic	407:412	arg1	composition					428:438	composition	428:438	composition	428:438	The osidic and proximate composition, structure conformation, flow behaviour, dynamic rheological and thermal properties of gums isolated from brown and golden flaxseeds were assessed.
34666134	3	35	theme	gum	794:796	arg1	extracts					798:805	the flaxseed gum extracts	781:805	the flaxseed gum extracts	781:805	Gum extraction under near-to-isoelectric point conditions did not impair the extraction yield, residual protein and ash content, whilst it resulted in minor changes in the sugar composition of the flaxseed gum extracts.
34666134	7	36	theme	better	1447:1452	arg1	stability					1462:1470	a better thermal stability	1445:1470	a better thermal stability	1445:1470	Golden flaxseed gums exhibited a better thermal stability compared to the brown flaxseed counterparts and therefore, they are suitable for product applications involving severe heat treatments.
34666134	0	37	dep	gums	79:82	arg1	extracted					84:92	extracted	84:92	gums extracted under alkaline and acidic conditions	79:129	Structure conformation, physicochemical and rheological properties of flaxseed gums extracted under alkaline and acidic conditions.
34666134	5	38	theme	hydrogen	1103:1110	arg1	interactions					1089:1100	the intra- and interchain polymer non-covalent interactions	1042:1100	the intra- and interchain polymer non-covalent interactions (hydrogen bonding) leading to a better solvation affinity in water and lyotropic solvents	1042:1190	The latter appeared to minimise the intra- and interchain polymer non-covalent interactions (hydrogen bonding) leading to a better solvation affinity in water and lyotropic solvents.
34666134	5	38	theme	hydrogen	1103:1110	arg1	bonding					1112:1118	hydrogen bonding	1103:1118	hydrogen bonding	1103:1118	The latter appeared to minimise the intra- and interchain polymer non-covalent interactions (hydrogen bonding) leading to a better solvation affinity in water and lyotropic solvents.
34666134	1	39	theme	consecutive	204:214	arg1	steps					216:220	consecutive steps	204:220	consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation	204:296	The present work aimed at investigating an extraction protocol based on consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation as an alternative method to produce flaxseed gum extracts of enhanced techno-functional characteristics.
34666134	2	40	theme	flow	465:468	arg1	behaviour					470:478	flow behaviour	465:478	flow behaviour	465:478	The osidic and proximate composition, structure conformation, flow behaviour, dynamic rheological and thermal properties of gums isolated from brown and golden flaxseeds were assessed.
34666134	4	41	theme	composite	989:997	arg1	fractions					999:1007	two AX-RG-I composite fractions	977:1007	two AX-RG-I composite fractions	977:1007	The deconvolution of the GPC/SEC chromatographs revealed the presence of four major polysaccharidic populations corresponding to arabinoxylans, rhamnogalacturonan-I and two AX-RG-I composite fractions.
34666134	6	42	theme	molecular	1229:1237	arg1	Mw = 1.34-1.15 × 106 Da					1247:1269	Mw = 1.34-1.15 × 106 Da	1247:1269	Mw = 1.34-1.15 × 106 Da	1247:1269	Golden flaxseed gums exerted higher molecular weight (Mw = 1.34-1.15 × 106 Da) and intrinsic viscosities (6.63-5.13 dL g-1) as well as better thickening and viscoelastic performance than the brown flaxseed gum exemplars.
34666134	6	42	theme	molecular	1229:1237	arg1	weight					1239:1244	higher molecular weight	1222:1244	higher molecular weight (Mw = 1.34-1.15 × 106 Da)	1222:1270	Golden flaxseed gums exerted higher molecular weight (Mw = 1.34-1.15 × 106 Da) and intrinsic viscosities (6.63-5.13 dL g-1) as well as better thickening and viscoelastic performance than the brown flaxseed gum exemplars.
34666134	0	43	theme	rheological	44:54	arg1	properties					56:65	rheological properties	44:65	rheological properties	44:65	Structure conformation, physicochemical and rheological properties of flaxseed gums extracted under alkaline and acidic conditions.
34666134	1	44	theme	alternative	304:314	arg1	protocol					186:193	an extraction protocol	172:193	an extraction protocol based on consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation	172:296	The present work aimed at investigating an extraction protocol based on consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation as an alternative method to produce flaxseed gum extracts of enhanced techno-functional characteristics.
34666134	1	44	theme	alternative	304:314	arg1	method					316:321	an alternative method	301:321	an alternative method to produce flaxseed gum extracts of enhanced techno-functional characteristics	301:400	The present work aimed at investigating an extraction protocol based on consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation as an alternative method to produce flaxseed gum extracts of enhanced techno-functional characteristics.
34666134	3	45	theme	ash	704:706	arg1	content					708:714	ash content	704:714	ash content	704:714	Gum extraction under near-to-isoelectric point conditions did not impair the extraction yield, residual protein and ash content, whilst it resulted in minor changes in the sugar composition of the flaxseed gum extracts.
34666134	4	46	theme	GPC/SEC	833:839	arg1	chromatographs					841:854	the GPC/SEC chromatographs	829:854	the GPC/SEC chromatographs	829:854	The deconvolution of the GPC/SEC chromatographs revealed the presence of four major polysaccharidic populations corresponding to arabinoxylans, rhamnogalacturonan-I and two AX-RG-I composite fractions.
34666134	7	47	theme	flaxseed	1421:1428	arg1	gums					1430:1433	Golden flaxseed gums	1414:1433	Golden flaxseed gums	1414:1433	Golden flaxseed gums exhibited a better thermal stability compared to the brown flaxseed counterparts and therefore, they are suitable for product applications involving severe heat treatments.
34666134	2	48	theme	structure	441:449	arg1	conformation					451:462	structure conformation	441:462	structure conformation	441:462	The osidic and proximate composition, structure conformation, flow behaviour, dynamic rheological and thermal properties of gums isolated from brown and golden flaxseeds were assessed.
34666134	2	49	theme	thermal	505:511	arg1	properties					513:522	dynamic rheological and thermal properties	481:522	dynamic rheological and thermal properties of gums isolated from brown	481:550	The osidic and proximate composition, structure conformation, flow behaviour, dynamic rheological and thermal properties of gums isolated from brown and golden flaxseeds were assessed.
34666134	6	50	theme	intrinsic	1276:1284	arg1	g-1					1312:1314	6.63-5.13 dL g-1	1299:1314	6.63-5.13 dL g-1	1299:1314	Golden flaxseed gums exerted higher molecular weight (Mw = 1.34-1.15 × 106 Da) and intrinsic viscosities (6.63-5.13 dL g-1) as well as better thickening and viscoelastic performance than the brown flaxseed gum exemplars.
34666134	6	50	theme	intrinsic	1276:1284	arg1	viscosities					1286:1296	intrinsic viscosities	1276:1296	intrinsic viscosities (6.63-5.13 dL g-1)	1276:1315	Golden flaxseed gums exerted higher molecular weight (Mw = 1.34-1.15 × 106 Da) and intrinsic viscosities (6.63-5.13 dL g-1) as well as better thickening and viscoelastic performance than the brown flaxseed gum exemplars.
34666134	3	51	theme	residual	683:690	arg1	protein					692:698	residual protein	683:698	residual protein	683:698	Gum extraction under near-to-isoelectric point conditions did not impair the extraction yield, residual protein and ash content, whilst it resulted in minor changes in the sugar composition of the flaxseed gum extracts.
34666134	7	52	theme	brown	1488:1492	arg1	counterparts					1503:1514	the brown flaxseed counterparts	1484:1514	the brown flaxseed counterparts	1484:1514	Golden flaxseed gums exhibited a better thermal stability compared to the brown flaxseed counterparts and therefore, they are suitable for product applications involving severe heat treatments.
34666134	1	53	theme	isoelectric	225:235	arg1	point					237:241	isoelectric point	225:241	isoelectric point	225:241	The present work aimed at investigating an extraction protocol based on consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation as an alternative method to produce flaxseed gum extracts of enhanced techno-functional characteristics.
34666134	6	54	theme	viscoelastic	1350:1361	arg1	performance					1363:1373	viscoelastic performance	1350:1373	viscoelastic performance	1350:1373	Golden flaxseed gums exerted higher molecular weight (Mw = 1.34-1.15 × 106 Da) and intrinsic viscosities (6.63-5.13 dL g-1) as well as better thickening and viscoelastic performance than the brown flaxseed gum exemplars.
34666134	6	55	theme	gum	1399:1401	arg1	exemplars					1403:1411	the brown flaxseed gum exemplars	1380:1411	the brown flaxseed gum exemplars	1380:1411	Golden flaxseed gums exerted higher molecular weight (Mw = 1.34-1.15 × 106 Da) and intrinsic viscosities (6.63-5.13 dL g-1) as well as better thickening and viscoelastic performance than the brown flaxseed gum exemplars.
34666134	6	56	theme	higher	1222:1227	arg1	Mw = 1.34-1.15 × 106 Da					1247:1269	Mw = 1.34-1.15 × 106 Da	1247:1269	Mw = 1.34-1.15 × 106 Da	1247:1269	Golden flaxseed gums exerted higher molecular weight (Mw = 1.34-1.15 × 106 Da) and intrinsic viscosities (6.63-5.13 dL g-1) as well as better thickening and viscoelastic performance than the brown flaxseed gum exemplars.
34666134	6	56	theme	higher	1222:1227	arg1	weight					1239:1244	higher molecular weight	1222:1244	higher molecular weight (Mw = 1.34-1.15 × 106 Da)	1222:1270	Golden flaxseed gums exerted higher molecular weight (Mw = 1.34-1.15 × 106 Da) and intrinsic viscosities (6.63-5.13 dL g-1) as well as better thickening and viscoelastic performance than the brown flaxseed gum exemplars.
34666134	2	57	theme	rheological	489:499	arg1	properties					513:522	dynamic rheological and thermal properties	481:522	dynamic rheological and thermal properties of gums isolated from brown	481:550	The osidic and proximate composition, structure conformation, flow behaviour, dynamic rheological and thermal properties of gums isolated from brown and golden flaxseeds were assessed.
34666134	3	58	theme	extracts	798:805	arg1	composition					766:776	the sugar composition	756:776	the sugar composition of the flaxseed gum extracts	756:805	Gum extraction under near-to-isoelectric point conditions did not impair the extraction yield, residual protein and ash content, whilst it resulted in minor changes in the sugar composition of the flaxseed gum extracts.
34666134	3	59	theme	minor	739:743	arg1	changes					745:751	minor changes	739:751	minor changes in the sugar composition of the flaxseed gum extracts	739:805	Gum extraction under near-to-isoelectric point conditions did not impair the extraction yield, residual protein and ash content, whilst it resulted in minor changes in the sugar composition of the flaxseed gum extracts.
34666134	7	60	theme	heat	1591:1594	arg1	treatments					1596:1605	severe heat treatments	1584:1605	severe heat treatments	1584:1605	Golden flaxseed gums exhibited a better thermal stability compared to the brown flaxseed counterparts and therefore, they are suitable for product applications involving severe heat treatments.
34666134	1	61	theme	flaxseed	334:341	arg1	extracts					347:354	flaxseed gum extracts	334:354	flaxseed gum extracts of enhanced techno-functional characteristics	334:400	The present work aimed at investigating an extraction protocol based on consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation as an alternative method to produce flaxseed gum extracts of enhanced techno-functional characteristics.
34666134	4	62	theme	AX-RG-I	981:987	arg1	fractions					999:1007	two AX-RG-I composite fractions	977:1007	two AX-RG-I composite fractions	977:1007	The deconvolution of the GPC/SEC chromatographs revealed the presence of four major polysaccharidic populations corresponding to arabinoxylans, rhamnogalacturonan-I and two AX-RG-I composite fractions.
34666134	7	63	theme	thermal	1454:1460	arg1	stability					1462:1470	a better thermal stability	1445:1470	a better thermal stability	1445:1470	Golden flaxseed gums exhibited a better thermal stability compared to the brown flaxseed counterparts and therefore, they are suitable for product applications involving severe heat treatments.
34666134	5	64	theme	polymer	1068:1074	arg1	interactions					1089:1100	the intra- and interchain polymer non-covalent interactions	1042:1100	the intra- and interchain polymer non-covalent interactions (hydrogen bonding) leading to a better solvation affinity in water and lyotropic solvents	1042:1190	The latter appeared to minimise the intra- and interchain polymer non-covalent interactions (hydrogen bonding) leading to a better solvation affinity in water and lyotropic solvents.
34666134	5	64	theme	polymer	1068:1074	arg1	bonding					1112:1118	hydrogen bonding	1103:1118	hydrogen bonding	1103:1118	The latter appeared to minimise the intra- and interchain polymer non-covalent interactions (hydrogen bonding) leading to a better solvation affinity in water and lyotropic solvents.
34666134	1	65	theme	present	136:142	arg1	work					144:147	The present work	132:147	The present work	132:147	The present work aimed at investigating an extraction protocol based on consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation as an alternative method to produce flaxseed gum extracts of enhanced techno-functional characteristics.
34666134	7	66	theme	flaxseed	1494:1501	arg1	counterparts					1503:1514	the brown flaxseed counterparts	1484:1514	the brown flaxseed counterparts	1484:1514	Golden flaxseed gums exhibited a better thermal stability compared to the brown flaxseed counterparts and therefore, they are suitable for product applications involving severe heat treatments.
34666134	6	67	theme	flaxseed	1390:1397	arg1	exemplars					1403:1411	the brown flaxseed gum exemplars	1380:1411	the brown flaxseed gum exemplars	1380:1411	Golden flaxseed gums exerted higher molecular weight (Mw = 1.34-1.15 × 106 Da) and intrinsic viscosities (6.63-5.13 dL g-1) as well as better thickening and viscoelastic performance than the brown flaxseed gum exemplars.
34666134	1	68	dep	mediated	255:262	arg1	point					237:241	isoelectric point	225:241	isoelectric point	225:241	The present work aimed at investigating an extraction protocol based on consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation as an alternative method to produce flaxseed gum extracts of enhanced techno-functional characteristics.
34666134	5	69	theme	non-covalent	1076:1087	arg1	interactions					1089:1100	the intra- and interchain polymer non-covalent interactions	1042:1100	the intra- and interchain polymer non-covalent interactions (hydrogen bonding) leading to a better solvation affinity in water and lyotropic solvents	1042:1190	The latter appeared to minimise the intra- and interchain polymer non-covalent interactions (hydrogen bonding) leading to a better solvation affinity in water and lyotropic solvents.
34666134	5	69	theme	non-covalent	1076:1087	arg1	bonding					1112:1118	hydrogen bonding	1103:1118	hydrogen bonding	1103:1118	The latter appeared to minimise the intra- and interchain polymer non-covalent interactions (hydrogen bonding) leading to a better solvation affinity in water and lyotropic solvents.
34666134	2	70	attach	isolated	532:539	arg1	brown					546:550	brown	546:550	brown	546:550	The osidic and proximate composition, structure conformation, flow behaviour, dynamic rheological and thermal properties of gums isolated from brown and golden flaxseeds were assessed.
34666134	2	70	attach	isolated	532:539	arg2	gums					527:530	gums	527:530	gums isolated from brown	527:550	The osidic and proximate composition, structure conformation, flow behaviour, dynamic rheological and thermal properties of gums isolated from brown and golden flaxseeds were assessed.
34666134	6	71	theme	flaxseed	1200:1207	arg1	gums					1209:1212	Golden flaxseed gums	1193:1212	Golden flaxseed gums	1193:1212	Golden flaxseed gums exerted higher molecular weight (Mw = 1.34-1.15 × 106 Da) and intrinsic viscosities (6.63-5.13 dL g-1) as well as better thickening and viscoelastic performance than the brown flaxseed gum exemplars.
34666134	5	72	theme	solvation	1141:1149	arg1	affinity					1151:1158	a better solvation affinity	1132:1158	a better solvation affinity in water and lyotropic solvents	1132:1190	The latter appeared to minimise the intra- and interchain polymer non-covalent interactions (hydrogen bonding) leading to a better solvation affinity in water and lyotropic solvents.
34666134	4	73	theme	chromatographs	841:854	arg1	deconvolution					812:824	The deconvolution	808:824	The deconvolution of the GPC/SEC chromatographs	808:854	The deconvolution of the GPC/SEC chromatographs revealed the presence of four major polysaccharidic populations corresponding to arabinoxylans, rhamnogalacturonan-I and two AX-RG-I composite fractions.
34666134	6	74	theme	Golden	1193:1198	arg1	gums					1209:1212	Golden flaxseed gums	1193:1212	Golden flaxseed gums	1193:1212	Golden flaxseed gums exerted higher molecular weight (Mw = 1.34-1.15 × 106 Da) and intrinsic viscosities (6.63-5.13 dL g-1) as well as better thickening and viscoelastic performance than the brown flaxseed gum exemplars.
34666134	0	75	theme	flaxseed	70:77	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure conformation, physicochemical and rheological properties of flaxseed gums extracted under alkaline and acidic conditions.
34666134	0	75	theme	flaxseed	70:77	arg1	physicochemical					24:38	physicochemical	24:38	physicochemical	24:38	Structure conformation, physicochemical and rheological properties of flaxseed gums extracted under alkaline and acidic conditions.
34666134	0	75	theme	flaxseed	70:77	arg1	properties					56:65	rheological properties	44:65	rheological properties	44:65	Structure conformation, physicochemical and rheological properties of flaxseed gums extracted under alkaline and acidic conditions.
34666134	1	76	theme	gum	343:345	arg1	extracts					347:354	flaxseed gum extracts	334:354	flaxseed gum extracts of enhanced techno-functional characteristics	334:400	The present work aimed at investigating an extraction protocol based on consecutive steps of isoelectric point (pH ~ 4.25) mediated gum swelling and deproteinisation as an alternative method to produce flaxseed gum extracts of enhanced techno-functional characteristics.
34666134	0	77	dep	Structure	0:8	arg1	conformation					10:21	conformation	10:21	conformation	10:21	Structure conformation, physicochemical and rheological properties of flaxseed gums extracted under alkaline and acidic conditions.
34666134	2	78	theme	golden	556:561	arg1	flaxseeds					563:571	golden flaxseeds	556:571	golden flaxseeds	556:571	The osidic and proximate composition, structure conformation, flow behaviour, dynamic rheological and thermal properties of gums isolated from brown and golden flaxseeds were assessed.
32729039	2	0	theme	angle	720:724	arg1	measurement					726:736	optical contact angle measurement	704:736	optical contact angle measurement	704:736	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	8	1	theme	endothermic	1531:1541	arg1	nature					1579:1584	the endothermic, spontaneous, and thermodynamically nature	1527:1584	the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process	1527:1617	Meanwhile, the maximum monolayer adsorption capacity (at pH = 5.0) was estimated to be 10.03 mg g-1 at 25 °C. Kinetic, isotherm, and thermodynamic studies were designated to proof the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process.
32729039	8	2	theme	adsorption	1600:1609	arg1	process					1611:1617	the phenol adsorption process	1589:1617	the phenol adsorption process	1589:1617	Meanwhile, the maximum monolayer adsorption capacity (at pH = 5.0) was estimated to be 10.03 mg g-1 at 25 °C. Kinetic, isotherm, and thermodynamic studies were designated to proof the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process.
32729039	1	3	theme	nanomagnetic	274:285	arg1	M-ZnO					299:303	M-ZnO	299:303	M-ZnO	299:303	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	1	3	theme	nanomagnetic	274:285	arg1	oxide					292:296	nanomagnetic zinc oxide	274:296	nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%)	274:359	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	2	4	dep	infrared	489:496	arg1	FTIR					499:502	FTIR	499:502	FTIR	499:502	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	2	5	theme	optical	704:710	arg1	measurement					726:736	optical contact angle measurement	704:736	optical contact angle measurement	704:736	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	8	6	theme	spontaneous	1544:1554	arg1	nature					1579:1584	the endothermic, spontaneous, and thermodynamically nature	1527:1584	the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process	1527:1617	Meanwhile, the maximum monolayer adsorption capacity (at pH = 5.0) was estimated to be 10.03 mg g-1 at 25 °C. Kinetic, isotherm, and thermodynamic studies were designated to proof the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process.
32729039	1	7	theme	oxide	292:296	arg1	ratios					264:269	six different ratios	250:269	six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%)	250:359	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	9	8	from	solutions	1755:1763	arg1	phenol					1735:1740	phenol	1735:1740	phenol from aqueous solutions	1735:1763	These outcomes indicate the effectiveness of the fabricated M-ZnO/PVA/Alg/CS nanofibers as adsorbent materials for phenol from aqueous solutions.
32729039	4	9	with	adsorbent	924:932	arg1	percentage					947:956	removal percentage	939:956	removal percentage of 84.22%	939:966	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers were selected as the best phenol adsorbent with removal percentage of 84.22%.
32729039	2	10	theme	sample	544:549	arg1	VSM					565:567	VSM	565:567	VSM	565:567	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	2	10	theme	sample	544:549	arg1	magnetometer					551:562	vibrating sample magnetometer	534:562	vibrating sample magnetometer (VSM)	534:568	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	6	11	theme	prepared	1244:1251	arg1	nanocomposites					1253:1266	the prepared nanocomposites	1240:1266	the prepared nanocomposites	1240:1266	Increasing nanofiber dosage and the solution temperature was found to enhance the phenol adsorption onto the prepared nanocomposites.
32729039	1	12	theme	0.8 wt	341:346	arg1	%					347:347	0.8 wt%	341:347	0.8 wt%	341:347	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	7	13	theme	removal	1302:1308	arg1	percentage					1281:1290	The maximum percentage	1269:1290	The maximum percentage of phenol removal	1269:1308	The maximum percentage of phenol removal was achieved at 84.22% after 90 min.
32729039	8	14	theme	maximum	1362:1368	arg1	capacity					1391:1398	the maximum monolayer adsorption capacity	1358:1398	the maximum monolayer adsorption capacity (at pH = 5.0)	1358:1412	Meanwhile, the maximum monolayer adsorption capacity (at pH = 5.0) was estimated to be 10.03 mg g-1 at 25 °C. Kinetic, isotherm, and thermodynamic studies were designated to proof the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process.
32729039	8	14	theme	maximum	1362:1368	arg1	Meanwhile					1347:1355	Meanwhile	1347:1355	Meanwhile	1347:1355	Meanwhile, the maximum monolayer adsorption capacity (at pH = 5.0) was estimated to be 10.03 mg g-1 at 25 °C. Kinetic, isotherm, and thermodynamic studies were designated to proof the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process.
32729039	8	14	theme	maximum	1362:1368	arg1	at					1401:1402	at pH = 5.0	1401:1411	at pH = 5.0	1401:1411	Meanwhile, the maximum monolayer adsorption capacity (at pH = 5.0) was estimated to be 10.03 mg g-1 at 25 °C. Kinetic, isotherm, and thermodynamic studies were designated to proof the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process.
32729039	3	15	from	solutions	830:838	arg1	adsorption					796:805	the adsorption	792:805	the adsorption of phenol from aqueous solutions	792:838	The fabricated composite nanofibers were applied for the adsorption of phenol from aqueous solutions.
32729039	2	16	theme	atomic	607:612	arg1	AFM					632:634	AFM	632:634	AFM	632:634	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	2	16	theme	atomic	607:612	arg1	microscope					620:629	atomic force microscope	607:629	atomic force microscope (AFM)	607:635	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	6	17	theme	phenol	1217:1222	arg1	adsorption					1224:1233	the phenol adsorption	1213:1233	the phenol adsorption onto the prepared nanocomposites	1213:1266	Increasing nanofiber dosage and the solution temperature was found to enhance the phenol adsorption onto the prepared nanocomposites.
32729039	1	18	theme	1 wt	354:357	arg1	%					358:358	1 wt%	354:358	1 wt%	354:358	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	1	19	dep	oxide	292:296	arg1	%					311:311	0 wt%	307:311	0 wt%	307:311	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	1	19	dep	oxide	292:296	arg1	%					338:338	0.6 wt%	332:338	0.6 wt%	332:338	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	1	19	dep	oxide	292:296	arg1	%					358:358	1 wt%	354:358	1 wt%	354:358	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	1	19	dep	oxide	292:296	arg1	%					320:320	0.2 wt%	314:320	0.2 wt%	314:320	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	1	19	dep	oxide	292:296	arg1	%					329:329	0.4 wt%	323:329	0.4 wt%	323:329	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	1	19	dep	oxide	292:296	arg1	%					347:347	0.8 wt%	341:347	0.8 wt%	341:347	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	3	20	theme	fabricated	743:752	arg1	nanofibers					764:773	The fabricated composite nanofibers	739:773	The fabricated composite nanofibers	739:773	The fabricated composite nanofibers were applied for the adsorption of phenol from aqueous solutions.
32729039	8	21	theme	adsorption	1380:1389	arg1	capacity					1391:1398	the maximum monolayer adsorption capacity	1358:1398	the maximum monolayer adsorption capacity (at pH = 5.0)	1358:1412	Meanwhile, the maximum monolayer adsorption capacity (at pH = 5.0) was estimated to be 10.03 mg g-1 at 25 °C. Kinetic, isotherm, and thermodynamic studies were designated to proof the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process.
32729039	8	21	theme	adsorption	1380:1389	arg1	Meanwhile					1347:1355	Meanwhile	1347:1355	Meanwhile	1347:1355	Meanwhile, the maximum monolayer adsorption capacity (at pH = 5.0) was estimated to be 10.03 mg g-1 at 25 °C. Kinetic, isotherm, and thermodynamic studies were designated to proof the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process.
32729039	8	21	theme	adsorption	1380:1389	arg1	at					1401:1402	at pH = 5.0	1401:1411	at pH = 5.0	1401:1411	Meanwhile, the maximum monolayer adsorption capacity (at pH = 5.0) was estimated to be 10.03 mg g-1 at 25 °C. Kinetic, isotherm, and thermodynamic studies were designated to proof the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process.
32729039	7	22	theme	maximum	1273:1279	arg1	percentage					1281:1290	The maximum percentage	1269:1290	The maximum percentage of phenol removal	1269:1308	The maximum percentage of phenol removal was achieved at 84.22% after 90 min.
32729039	1	23	theme	poly	158:161	arg1	nanofiber					219:227	poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber	158:227	poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber	158:227	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	4	24	theme	%	966:966	arg1	percentage					947:956	removal percentage	939:956	removal percentage of 84.22%	939:966	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers were selected as the best phenol adsorbent with removal percentage of 84.22%.
32729039	5	25	theme	contact	1025:1031	arg1	time					1033:1036	contact time	1025:1036	contact time	1025:1036	The influence of different processing parameter such as contact time, composite nanofiber dosage, pH, initial pollutant concentration, and temperature were examined.
32729039	1	26	theme	vinyl	163:167	arg1	nanofiber					219:227	poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber	158:227	poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber	158:227	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	9	27	theme	M-ZnO/PVA/Alg/CS	1680:1695	arg1	nanofibers					1697:1706	the fabricated M-ZnO/PVA/Alg/CS nanofibers	1665:1706	the fabricated M-ZnO/PVA/Alg/CS nanofibers	1665:1706	These outcomes indicate the effectiveness of the fabricated M-ZnO/PVA/Alg/CS nanofibers as adsorbent materials for phenol from aqueous solutions.
32729039	0	28	theme	enhanced	103:110	arg1	decontamination					119:133	enhanced phenol decontamination	103:133	enhanced phenol decontamination	103:133	Novel eco-friendly electrospun nanomagnetic zinc oxide hybridized PVA/alginate/chitosan nanofibers for enhanced phenol decontamination.
32729039	1	29	theme	0.4 wt	323:328	arg1	%					329:329	0.4 wt%	323:329	0.4 wt%	323:329	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	4	30	theme	1.0 wt	845:850	arg1	%					851:851	%	851:851	%	851:851	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers were selected as the best phenol adsorbent with removal percentage of 84.22%.
32729039	5	31	theme	processing	996:1005	arg1	parameter					1007:1015	different processing parameter	986:1015	different processing parameter	986:1015	The influence of different processing parameter such as contact time, composite nanofiber dosage, pH, initial pollutant concentration, and temperature were examined.
32729039	4	32	theme	M-ZnO/PVA/Alg/CS	853:868	arg1	nanofibers					880:889	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers	841:889	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers	841:889	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers were selected as the best phenol adsorbent with removal percentage of 84.22%.
32729039	4	32	theme	M-ZnO/PVA/Alg/CS	853:868	arg1	phenol					917:922	the best phenol	908:922	the best phenol adsorbent with removal percentage of 84.22%	908:966	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers were selected as the best phenol adsorbent with removal percentage of 84.22%.
32729039	0	33	theme	Novel	0:4	arg1	oxide					49:53	Novel eco-friendly electrospun nanomagnetic zinc oxide	0:53	Novel eco-friendly electrospun nanomagnetic zinc oxide	0:53	Novel eco-friendly electrospun nanomagnetic zinc oxide hybridized PVA/alginate/chitosan nanofibers for enhanced phenol decontamination.
32729039	9	34	theme	aqueous	1747:1753	arg1	solutions					1755:1763	aqueous solutions	1747:1763	aqueous solutions	1747:1763	These outcomes indicate the effectiveness of the fabricated M-ZnO/PVA/Alg/CS nanofibers as adsorbent materials for phenol from aqueous solutions.
32729039	2	35	theme	electron	580:587	arg1	microscope					589:598	scanning electron microscope	571:598	scanning electron microscope (SEM)	571:604	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	2	35	theme	electron	580:587	arg1	SEM					601:603	SEM	601:603	SEM	601:603	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	0	36	theme	electrospun	19:29	arg1	oxide					49:53	Novel eco-friendly electrospun nanomagnetic zinc oxide	0:53	Novel eco-friendly electrospun nanomagnetic zinc oxide	0:53	Novel eco-friendly electrospun nanomagnetic zinc oxide hybridized PVA/alginate/chitosan nanofibers for enhanced phenol decontamination.
32729039	5	37	theme	pollutant	1079:1087	arg1	concentration					1089:1101	initial pollutant concentration	1071:1101	initial pollutant concentration	1071:1101	The influence of different processing parameter such as contact time, composite nanofiber dosage, pH, initial pollutant concentration, and temperature were examined.
32729039	2	38	theme	composite	419:427	arg1	nanofibers					435:444	The various fabricated composite (M-6) nanofibers	396:444	The various fabricated composite (M-6) nanofibers	396:444	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	0	39	theme	zinc	44:47	arg1	oxide					49:53	Novel eco-friendly electrospun nanomagnetic zinc oxide	0:53	Novel eco-friendly electrospun nanomagnetic zinc oxide	0:53	Novel eco-friendly electrospun nanomagnetic zinc oxide hybridized PVA/alginate/chitosan nanofibers for enhanced phenol decontamination.
32729039	1	40	theme	0 wt	307:310	arg1	%					311:311	0 wt%	307:311	0 wt%	307:311	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	2	41	theme	various	400:406	arg1	nanofibers					435:444	The various fabricated composite (M-6) nanofibers	396:444	The various fabricated composite (M-6) nanofibers	396:444	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	5	42	theme	nanofiber	1049:1057	arg1	dosage					1059:1064	composite nanofiber dosage	1039:1064	composite nanofiber dosage	1039:1064	The influence of different processing parameter such as contact time, composite nanofiber dosage, pH, initial pollutant concentration, and temperature were examined.
32729039	9	43	theme	fabricated	1669:1678	arg1	nanofibers					1697:1706	the fabricated M-ZnO/PVA/Alg/CS nanofibers	1665:1706	the fabricated M-ZnO/PVA/Alg/CS nanofibers	1665:1706	These outcomes indicate the effectiveness of the fabricated M-ZnO/PVA/Alg/CS nanofibers as adsorbent materials for phenol from aqueous solutions.
32729039	8	44	theme	thermodynamic	1480:1492	arg1	studies					1494:1500	25 °C. Kinetic, isotherm, and thermodynamic studies	1450:1500	25 °C. Kinetic, isotherm, and thermodynamic studies	1450:1500	Meanwhile, the maximum monolayer adsorption capacity (at pH = 5.0) was estimated to be 10.03 mg g-1 at 25 °C. Kinetic, isotherm, and thermodynamic studies were designated to proof the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process.
32729039	8	45	theme	thermodynamically	1561:1577	arg1	nature					1579:1584	the endothermic, spontaneous, and thermodynamically nature	1527:1584	the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process	1527:1617	Meanwhile, the maximum monolayer adsorption capacity (at pH = 5.0) was estimated to be 10.03 mg g-1 at 25 °C. Kinetic, isotherm, and thermodynamic studies were designated to proof the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process.
32729039	1	46	theme	different	254:262	arg1	ratios					264:269	six different ratios	250:269	six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%)	250:359	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	2	47	theme	contact	712:718	arg1	measurement					726:736	optical contact angle measurement	704:736	optical contact angle measurement	704:736	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	1	48	theme	electrospinning	369:383	arg1	technique					385:393	the electrospinning technique	365:393	the electrospinning technique	365:393	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	8	49	theme	phenol	1593:1598	arg1	process					1611:1617	the phenol adsorption process	1589:1617	the phenol adsorption process	1589:1617	Meanwhile, the maximum monolayer adsorption capacity (at pH = 5.0) was estimated to be 10.03 mg g-1 at 25 °C. Kinetic, isotherm, and thermodynamic studies were designated to proof the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process.
32729039	1	50	theme	zinc	287:290	arg1	M-ZnO					299:303	M-ZnO	299:303	M-ZnO	299:303	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	1	50	theme	zinc	287:290	arg1	oxide					292:296	nanomagnetic zinc oxide	274:296	nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%)	274:359	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	2	51	dep	transform	479:487	arg1	infrared					489:496	infrared	489:496	infrared	489:496	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	6	52	theme	solution	1171:1178	arg1	temperature					1180:1190	the solution temperature	1167:1190	the solution temperature	1167:1190	Increasing nanofiber dosage and the solution temperature was found to enhance the phenol adsorption onto the prepared nanocomposites.
32729039	8	53	theme	process	1611:1617	arg1	nature					1579:1584	the endothermic, spontaneous, and thermodynamically nature	1527:1584	the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process	1527:1617	Meanwhile, the maximum monolayer adsorption capacity (at pH = 5.0) was estimated to be 10.03 mg g-1 at 25 °C. Kinetic, isotherm, and thermodynamic studies were designated to proof the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process.
32729039	1	54	theme	/alginate/chitosan	177:194	arg1	nanofiber					219:227	poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber	158:227	poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber	158:227	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	2	55	theme	thermogravimetric	638:654	arg1	analysis					656:663	thermogravimetric analysis	638:663	thermogravimetric analysis (TGA)	638:669	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	2	55	theme	thermogravimetric	638:654	arg1	TGA					666:668	TGA	666:668	TGA	666:668	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	1	56	theme	PVA/Alg/CS	197:206	arg1	nanofiber					219:227	poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber	158:227	poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber	158:227	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	2	57	theme	vibrating	534:542	arg1	VSM					565:567	VSM	565:567	VSM	565:567	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	2	57	theme	vibrating	534:542	arg1	magnetometer					551:562	vibrating sample magnetometer	534:562	vibrating sample magnetometer (VSM)	534:568	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	2	58	theme	mechanical	672:681	arg1	machine					691:697	mechanical testing machine	672:697	mechanical testing machine	672:697	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	1	59	theme	composite	209:217	arg1	nanofiber					219:227	poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber	158:227	poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber	158:227	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	8	60	theme	monolayer	1370:1378	arg1	capacity					1391:1398	the maximum monolayer adsorption capacity	1358:1398	the maximum monolayer adsorption capacity (at pH = 5.0)	1358:1412	Meanwhile, the maximum monolayer adsorption capacity (at pH = 5.0) was estimated to be 10.03 mg g-1 at 25 °C. Kinetic, isotherm, and thermodynamic studies were designated to proof the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process.
32729039	8	60	theme	monolayer	1370:1378	arg1	Meanwhile					1347:1355	Meanwhile	1347:1355	Meanwhile	1347:1355	Meanwhile, the maximum monolayer adsorption capacity (at pH = 5.0) was estimated to be 10.03 mg g-1 at 25 °C. Kinetic, isotherm, and thermodynamic studies were designated to proof the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process.
32729039	8	60	theme	monolayer	1370:1378	arg1	at					1401:1402	at pH = 5.0	1401:1411	at pH = 5.0	1401:1411	Meanwhile, the maximum monolayer adsorption capacity (at pH = 5.0) was estimated to be 10.03 mg g-1 at 25 °C. Kinetic, isotherm, and thermodynamic studies were designated to proof the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process.
32729039	6	61	theme	Increasing	1135:1144	arg1	dosage					1156:1161	Increasing nanofiber dosage	1135:1161	Increasing nanofiber dosage	1135:1161	Increasing nanofiber dosage and the solution temperature was found to enhance the phenol adsorption onto the prepared nanocomposites.
32729039	2	62	theme	force	614:618	arg1	AFM					632:634	AFM	632:634	AFM	632:634	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	2	62	theme	force	614:618	arg1	microscope					620:629	atomic force microscope	607:629	atomic force microscope (AFM)	607:635	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	5	63	theme	different	986:994	arg1	parameter					1007:1015	different processing parameter	986:1015	different processing parameter	986:1015	The influence of different processing parameter such as contact time, composite nanofiber dosage, pH, initial pollutant concentration, and temperature were examined.
32729039	1	64	theme	current	143:149	arg1	study					151:155	the current study	139:155	the current study	139:155	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	2	65	theme	X-ray	506:510	arg1	XRD					528:530	XRD	528:530	XRD	528:530	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	2	65	theme	X-ray	506:510	arg1	diffractometer					512:525	X-ray diffractometer	506:525	X-ray diffractometer (XRD)	506:531	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	7	66	theme	phenol	1295:1300	arg1	removal					1302:1308	phenol removal	1295:1308	phenol removal	1295:1308	The maximum percentage of phenol removal was achieved at 84.22% after 90 min.
32729039	3	67	theme	composite	754:762	arg1	nanofibers					764:773	The fabricated composite nanofibers	739:773	The fabricated composite nanofibers	739:773	The fabricated composite nanofibers were applied for the adsorption of phenol from aqueous solutions.
32729039	4	68	theme	composite	870:878	arg1	nanofibers					880:889	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers	841:889	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers	841:889	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers were selected as the best phenol adsorbent with removal percentage of 84.22%.
32729039	4	68	theme	composite	870:878	arg1	phenol					917:922	the best phenol	908:922	the best phenol adsorbent with removal percentage of 84.22%	908:966	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers were selected as the best phenol adsorbent with removal percentage of 84.22%.
32729039	0	69	theme	phenol	112:117	arg1	decontamination					119:133	enhanced phenol decontamination	103:133	enhanced phenol decontamination	103:133	Novel eco-friendly electrospun nanomagnetic zinc oxide hybridized PVA/alginate/chitosan nanofibers for enhanced phenol decontamination.
32729039	1	70	theme	alcohol	169:175	arg1	nanofiber					219:227	poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber	158:227	poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber	158:227	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	9	71	theme	nanofibers	1697:1706	arg1	effectiveness					1648:1660	the effectiveness	1644:1660	the effectiveness of the fabricated M-ZnO/PVA/Alg/CS nanofibers as adsorbent materials for phenol from aqueous solutions	1644:1763	These outcomes indicate the effectiveness of the fabricated M-ZnO/PVA/Alg/CS nanofibers as adsorbent materials for phenol from aqueous solutions.
32729039	2	72	theme	scanning	571:578	arg1	microscope					589:598	scanning electron microscope	571:598	scanning electron microscope (SEM)	571:604	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	2	72	theme	scanning	571:578	arg1	SEM					601:603	SEM	601:603	SEM	601:603	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	5	73	theme	composite	1039:1047	arg1	dosage					1059:1064	composite nanofiber dosage	1039:1064	composite nanofiber dosage	1039:1064	The influence of different processing parameter such as contact time, composite nanofiber dosage, pH, initial pollutant concentration, and temperature were examined.
32729039	9	74	theme	adsorbent	1711:1719	arg1	materials					1721:1729	adsorbent materials	1711:1729	adsorbent materials for phenol from aqueous solutions	1711:1763	These outcomes indicate the effectiveness of the fabricated M-ZnO/PVA/Alg/CS nanofibers as adsorbent materials for phenol from aqueous solutions.
32729039	1	75	theme	0.6 wt	332:337	arg1	%					338:338	0.6 wt%	332:338	0.6 wt%	332:338	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	3	76	theme	phenol	810:815	arg1	adsorption					796:805	the adsorption	792:805	the adsorption of phenol from aqueous solutions	792:838	The fabricated composite nanofibers were applied for the adsorption of phenol from aqueous solutions.
32729039	4	77	theme	%	851:851	arg1	nanofibers					880:889	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers	841:889	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers	841:889	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers were selected as the best phenol adsorbent with removal percentage of 84.22%.
32729039	4	77	theme	%	851:851	arg1	phenol					917:922	the best phenol	908:922	the best phenol adsorbent with removal percentage of 84.22%	908:966	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers were selected as the best phenol adsorbent with removal percentage of 84.22%.
32729039	0	78	theme	eco-friendly	6:17	arg1	oxide					49:53	Novel eco-friendly electrospun nanomagnetic zinc oxide	0:53	Novel eco-friendly electrospun nanomagnetic zinc oxide	0:53	Novel eco-friendly electrospun nanomagnetic zinc oxide hybridized PVA/alginate/chitosan nanofibers for enhanced phenol decontamination.
32729039	5	79	theme	parameter	1007:1015	arg1	influence					973:981	The influence	969:981	The influence of different processing parameter such as contact time, composite nanofiber dosage, pH, initial pollutant concentration, and temperature	969:1118	The influence of different processing parameter such as contact time, composite nanofiber dosage, pH, initial pollutant concentration, and temperature were examined.
32729039	4	80	theme	adsorbent	924:932	arg1	nanofibers					880:889	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers	841:889	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers	841:889	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers were selected as the best phenol adsorbent with removal percentage of 84.22%.
32729039	4	80	theme	adsorbent	924:932	arg1	phenol					917:922	the best phenol	908:922	the best phenol adsorbent with removal percentage of 84.22%	908:966	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers were selected as the best phenol adsorbent with removal percentage of 84.22%.
32729039	8	81	theme	Kinetic	1457:1463	arg1	studies					1494:1500	25 °C. Kinetic, isotherm, and thermodynamic studies	1450:1500	25 °C. Kinetic, isotherm, and thermodynamic studies	1450:1500	Meanwhile, the maximum monolayer adsorption capacity (at pH = 5.0) was estimated to be 10.03 mg g-1 at 25 °C. Kinetic, isotherm, and thermodynamic studies were designated to proof the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process.
32729039	0	82	theme	nanomagnetic	31:42	arg1	oxide					49:53	Novel eco-friendly electrospun nanomagnetic zinc oxide	0:53	Novel eco-friendly electrospun nanomagnetic zinc oxide	0:53	Novel eco-friendly electrospun nanomagnetic zinc oxide hybridized PVA/alginate/chitosan nanofibers for enhanced phenol decontamination.
32729039	5	83	theme	initial	1071:1077	arg1	concentration					1089:1101	initial pollutant concentration	1071:1101	initial pollutant concentration	1071:1101	The influence of different processing parameter such as contact time, composite nanofiber dosage, pH, initial pollutant concentration, and temperature were examined.
32729039	6	84	theme	nanofiber	1146:1154	arg1	dosage					1156:1161	Increasing nanofiber dosage	1135:1161	Increasing nanofiber dosage	1135:1161	Increasing nanofiber dosage and the solution temperature was found to enhance the phenol adsorption onto the prepared nanocomposites.
32729039	4	85	theme	removal	939:945	arg1	percentage					947:956	removal percentage	939:956	removal percentage of 84.22%	939:966	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers were selected as the best phenol adsorbent with removal percentage of 84.22%.
32729039	8	86	theme	isotherm	1466:1473	arg1	studies					1494:1500	25 °C. Kinetic, isotherm, and thermodynamic studies	1450:1500	25 °C. Kinetic, isotherm, and thermodynamic studies	1450:1500	Meanwhile, the maximum monolayer adsorption capacity (at pH = 5.0) was estimated to be 10.03 mg g-1 at 25 °C. Kinetic, isotherm, and thermodynamic studies were designated to proof the endothermic, spontaneous, and thermodynamically nature of the phenol adsorption process.
32729039	2	87	dep	Fourier	471:477	arg1	transform					479:487	transform	479:487	transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement	479:736	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	3	88	theme	aqueous	822:828	arg1	solutions					830:838	aqueous solutions	822:838	aqueous solutions	822:838	The fabricated composite nanofibers were applied for the adsorption of phenol from aqueous solutions.
32729039	2	89	theme	fabricated	408:417	arg1	nanofibers					435:444	The various fabricated composite (M-6) nanofibers	396:444	The various fabricated composite (M-6) nanofibers	396:444	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
32729039	0	90	theme	PVA/alginate/chitosan	66:86	arg1	nanofibers					88:97	PVA/alginate/chitosan nanofibers	66:97	PVA/alginate/chitosan nanofibers for enhanced phenol decontamination	66:133	Novel eco-friendly electrospun nanomagnetic zinc oxide hybridized PVA/alginate/chitosan nanofibers for enhanced phenol decontamination.
32729039	4	91	theme	best	912:915	arg1	nanofibers					880:889	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers	841:889	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers	841:889	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers were selected as the best phenol adsorbent with removal percentage of 84.22%.
32729039	4	91	theme	best	912:915	arg1	phenol					917:922	the best phenol	908:922	the best phenol adsorbent with removal percentage of 84.22%	908:966	The 1.0 wt% M-ZnO/PVA/Alg/CS composite nanofibers were selected as the best phenol adsorbent with removal percentage of 84.22%.
32729039	1	92	theme	0.2 wt	314:319	arg1	%					320:320	0.2 wt%	314:320	0.2 wt%	314:320	In the current study, poly(vinyl alcohol)/alginate/chitosan (PVA/Alg/CS) composite nanofiber was immobilized with six different ratios of nanomagnetic zinc oxide (M-ZnO) (0 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, and 1 wt%) via the electrospinning technique.
32729039	2	93	theme	testing	683:689	arg1	machine					691:697	mechanical testing machine	672:697	mechanical testing machine	672:697	The various fabricated composite (M-6) nanofibers were characterized using Fourier transform infrared (FTIR), X-ray diffractometer (XRD), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), atomic force microscope (AFM), thermogravimetric analysis (TGA), mechanical testing machine, and optical contact angle measurement.
33807434	0	0	theme	Bone	99:102	arg1	Engineering					111:121	Bone Tissue Engineering	99:121	Bone Tissue Engineering	99:121	Mechanical, Structural, and Biological Properties of Chitosan/Hydroxyapatite/Silica Composites for Bone Tissue Engineering.
33807434	1	1	theme	hydroxyapatite	335:348	arg1	powder					350:355	hydroxyapatite powder	335:355	hydroxyapatite powder (HAp)	335:361	The aim of this work was to fabricate novel bioactive composites based on chitosan and non-organic silica, reinforced with calcium β-glycerophosphate (Ca-GP), sodium β-glycerophosphate pentahydrate (Na-GP), and hydroxyapatite powder (HAp) in a range of concentrations using the sol-gel method.
33807434	1	1	theme	hydroxyapatite	335:348	arg1	HAp					358:360	HAp	358:360	HAp	358:360	The aim of this work was to fabricate novel bioactive composites based on chitosan and non-organic silica, reinforced with calcium β-glycerophosphate (Ca-GP), sodium β-glycerophosphate pentahydrate (Na-GP), and hydroxyapatite powder (HAp) in a range of concentrations using the sol-gel method.
33807434	5	2	theme	volumetric	1146:1155	arg1	shrinkage					1157:1165	volumetric shrinkage	1146:1165	volumetric shrinkage	1146:1165	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	2	3	theme	Ca-GP	448:452	arg1	contents					454:461	Ca-GP contents	448:461	Ca-GP contents	448:461	The effect of HAp, Na-GP, and Ca-GP contents on the mechanical properties, i.e., Young's modulus, compressive strength, and yield strain, of hybrid composites was analyzed.
33807434	5	4	dep	10	1097:1098	arg1	to					1094:1095	to	1094:1095	to	1094:1095	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	2	5	theme	contents	454:461	arg1	effect					422:427	The effect	418:427	The effect of HAp, Na-GP, and Ca-GP contents on the mechanical properties, i.e., Young's modulus, compressive strength, and yield strain, of hybrid composites	418:575	The effect of HAp, Na-GP, and Ca-GP contents on the mechanical properties, i.e., Young's modulus, compressive strength, and yield strain, of hybrid composites was analyzed.
33807434	1	6	theme	novel	162:166	arg1	composites					178:187	novel bioactive composites	162:187	novel bioactive composites	162:187	The aim of this work was to fabricate novel bioactive composites based on chitosan and non-organic silica, reinforced with calcium β-glycerophosphate (Ca-GP), sodium β-glycerophosphate pentahydrate (Na-GP), and hydroxyapatite powder (HAp) in a range of concentrations using the sol-gel method.
33807434	6	7	from	applications	1222:1233	arg1	engineering					1245:1255	tissue engineering	1238:1255	tissue engineering	1238:1255	This proved to be a promising composite for applications in tissue engineering, e.g., filling small bone defects.
33807434	2	8	theme	yield	542:546	arg1	strain					548:553	yield strain	542:553	yield strain	542:553	The effect of HAp, Na-GP, and Ca-GP contents on the mechanical properties, i.e., Young's modulus, compressive strength, and yield strain, of hybrid composites was analyzed.
33807434	5	9	from	shrinkage	1157:1165	arg1	range					1081:1085	a range	1079:1085	a range of 0.3 to 10 MPa	1079:1102	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	0	10	theme	Tissue	104:109	arg1	Engineering					111:121	Bone Tissue Engineering	99:121	Bone Tissue Engineering	99:121	Mechanical, Structural, and Biological Properties of Chitosan/Hydroxyapatite/Silica Composites for Bone Tissue Engineering.
33807434	5	11	theme	μm	930:931	arg1	ones					918:921	large ones	912:921	large ones of 100 μm	912:931	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	5	11	theme	μm	930:931	arg1	pores					950:954	many smaller pores	937:954	many smaller pores below 10 µm	937:966	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	4	12	theme	FTIR	709:712	arg1	analysis					714:721	FTIR analysis	709:721	FTIR analysis	709:721	Moreover, the molecular interactions according to FTIR analysis and biocompatibility of composites obtained were examined.
33807434	5	13	theme	sizes	905:909	arg1	pores					892:896	pores	892:896	pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%	892:1175	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	2	14	dep	modulus	507:513	arg1	i.e.					493:496	i.e.	493:496	i.e.	493:496	The effect of HAp, Na-GP, and Ca-GP contents on the mechanical properties, i.e., Young's modulus, compressive strength, and yield strain, of hybrid composites was analyzed.
33807434	1	15	theme	bioactive	168:176	arg1	composites					178:187	novel bioactive composites	162:187	novel bioactive composites	162:187	The aim of this work was to fabricate novel bioactive composites based on chitosan and non-organic silica, reinforced with calcium β-glycerophosphate (Ca-GP), sodium β-glycerophosphate pentahydrate (Na-GP), and hydroxyapatite powder (HAp) in a range of concentrations using the sol-gel method.
33807434	2	16	theme	mechanical	470:479	arg1	properties					481:490	the mechanical properties	466:490	the mechanical properties	466:490	The effect of HAp, Na-GP, and Ca-GP contents on the mechanical properties, i.e., Young's modulus, compressive strength, and yield strain, of hybrid composites was analyzed.
33807434	6	17	dep	defects	1283:1289	arg1	e.g.					1258:1261	e.g.	1258:1261	e.g.	1258:1261	This proved to be a promising composite for applications in tissue engineering, e.g., filling small bone defects.
33807434	2	18	theme	composites	566:575	arg1	properties					481:490	the mechanical properties	466:490	the mechanical properties	466:490	The effect of HAp, Na-GP, and Ca-GP contents on the mechanical properties, i.e., Young's modulus, compressive strength, and yield strain, of hybrid composites was analyzed.
33807434	5	19	theme	many	937:940	arg1	pores					950:954	many smaller pores	937:954	many smaller pores below 10 µm	937:966	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	6	20	theme	tissue	1238:1243	arg1	engineering					1245:1255	tissue engineering	1238:1255	tissue engineering	1238:1255	This proved to be a promising composite for applications in tissue engineering, e.g., filling small bone defects.
33807434	2	21	theme	HAp	432:434	arg1	effect					422:427	The effect	418:427	The effect of HAp, Na-GP, and Ca-GP contents on the mechanical properties, i.e., Young's modulus, compressive strength, and yield strain, of hybrid composites	418:575	The effect of HAp, Na-GP, and Ca-GP contents on the mechanical properties, i.e., Young's modulus, compressive strength, and yield strain, of hybrid composites was analyzed.
33807434	2	22	theme	hybrid	559:564	arg1	composites					566:575	hybrid composites	559:575	hybrid composites	559:575	The effect of HAp, Na-GP, and Ca-GP contents on the mechanical properties, i.e., Young's modulus, compressive strength, and yield strain, of hybrid composites was analyzed.
33807434	6	23	theme	small	1272:1276	arg1	defects					1283:1289	filling small bone defects	1264:1289	filling small bone defects	1264:1289	This proved to be a promising composite for applications in tissue engineering, e.g., filling small bone defects.
33807434	0	24	theme	Mechanical	0:9	arg1	Properties					39:48	Mechanical, Structural, and Biological Properties	0:48	Mechanical, Structural, and Biological Properties of Chitosan/Hydroxyapatite/Silica Composites for Bone Tissue Engineering	0:121	Mechanical, Structural, and Biological Properties of Chitosan/Hydroxyapatite/Silica Composites for Bone Tissue Engineering.
33807434	4	25	theme	molecular	673:681	arg1	interactions					683:694	the molecular interactions	669:694	the molecular interactions according to FTIR analysis and biocompatibility of composites obtained	669:765	Moreover, the molecular interactions according to FTIR analysis and biocompatibility of composites obtained were examined.
33807434	1	26	theme	sodium	283:288	arg1	Na-GP					323:327	Na-GP	323:327	Na-GP	323:327	The aim of this work was to fabricate novel bioactive composites based on chitosan and non-organic silica, reinforced with calcium β-glycerophosphate (Ca-GP), sodium β-glycerophosphate pentahydrate (Na-GP), and hydroxyapatite powder (HAp) in a range of concentrations using the sol-gel method.
33807434	1	26	theme	sodium	283:288	arg1	pentahydrate					309:320	sodium β-glycerophosphate pentahydrate	283:320	sodium β-glycerophosphate pentahydrate (Na-GP)	283:328	The aim of this work was to fabricate novel bioactive composites based on chitosan and non-organic silica, reinforced with calcium β-glycerophosphate (Ca-GP), sodium β-glycerophosphate pentahydrate (Na-GP), and hydroxyapatite powder (HAp) in a range of concentrations using the sol-gel method.
33807434	5	27	dep	100	1133:1135	arg1	to					1130:1131	to	1130:1131	to	1130:1131	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	5	28	theme	ones	918:921	arg1	behavior					973:980	the behavior	969:980	the behavior	969:980	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	0	29	theme	Structural	12:21	arg1	Properties					39:48	Mechanical, Structural, and Biological Properties	0:48	Mechanical, Structural, and Biological Properties of Chitosan/Hydroxyapatite/Silica Composites for Bone Tissue Engineering	0:121	Mechanical, Structural, and Biological Properties of Chitosan/Hydroxyapatite/Silica Composites for Bone Tissue Engineering.
33807434	1	30	theme	β-glycerophosphate	290:307	arg1	Na-GP					323:327	Na-GP	323:327	Na-GP	323:327	The aim of this work was to fabricate novel bioactive composites based on chitosan and non-organic silica, reinforced with calcium β-glycerophosphate (Ca-GP), sodium β-glycerophosphate pentahydrate (Na-GP), and hydroxyapatite powder (HAp) in a range of concentrations using the sol-gel method.
33807434	1	30	theme	β-glycerophosphate	290:307	arg1	pentahydrate					309:320	sodium β-glycerophosphate pentahydrate	283:320	sodium β-glycerophosphate pentahydrate (Na-GP)	283:328	The aim of this work was to fabricate novel bioactive composites based on chitosan and non-organic silica, reinforced with calcium β-glycerophosphate (Ca-GP), sodium β-glycerophosphate pentahydrate (Na-GP), and hydroxyapatite powder (HAp) in a range of concentrations using the sol-gel method.
33807434	5	31	theme	large	912:916	arg1	ones					918:921	large ones	912:921	large ones of 100 μm	912:931	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	5	32	theme	cell	1013:1016	arg1	proliferation					1018:1030	cell proliferation	1013:1030	cell proliferation	1013:1030	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	0	33	theme	Biological	28:37	arg1	Properties					39:48	Mechanical, Structural, and Biological Properties	0:48	Mechanical, Structural, and Biological Properties of Chitosan/Hydroxyapatite/Silica Composites for Bone Tissue Engineering	0:121	Mechanical, Structural, and Biological Properties of Chitosan/Hydroxyapatite/Silica Composites for Bone Tissue Engineering.
33807434	5	34	theme	Young	1105:1109	arg1	modulus					1113:1119	Young's modulus	1105:1119	Young's modulus from 5.2 to 100 MPa	1105:1139	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	2	35	theme	Young	499:503	arg1	modulus					507:513	Young's modulus	499:513	Young's modulus	499:513	The effect of HAp, Na-GP, and Ca-GP contents on the mechanical properties, i.e., Young's modulus, compressive strength, and yield strain, of hybrid composites was analyzed.
33807434	1	36	theme	non-organic	211:221	arg1	silica					223:228	non-organic silica	211:228	non-organic silica	211:228	The aim of this work was to fabricate novel bioactive composites based on chitosan and non-organic silica, reinforced with calcium β-glycerophosphate (Ca-GP), sodium β-glycerophosphate pentahydrate (Na-GP), and hydroxyapatite powder (HAp) in a range of concentrations using the sol-gel method.
33807434	6	37	theme	filling	1264:1270	arg1	defects					1283:1289	filling small bone defects	1264:1289	filling small bone defects	1264:1289	This proved to be a promising composite for applications in tissue engineering, e.g., filling small bone defects.
33807434	5	38	from	modulus	1113:1119	arg1	range					1081:1085	a range	1079:1085	a range of 0.3 to 10 MPa	1079:1102	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	1	39	theme	concentrations	377:390	arg1	range					368:372	a range	366:372	a range of concentrations using the sol-gel method	366:415	The aim of this work was to fabricate novel bioactive composites based on chitosan and non-organic silica, reinforced with calcium β-glycerophosphate (Ca-GP), sodium β-glycerophosphate pentahydrate (Na-GP), and hydroxyapatite powder (HAp) in a range of concentrations using the sol-gel method.
33807434	5	40	from	proliferation	1018:1030	arg1	range					1081:1085	a range	1079:1085	a range of 0.3 to 10 MPa	1079:1102	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	2	41	theme	Na-GP	437:441	arg1	effect					422:427	The effect	418:427	The effect of HAp, Na-GP, and Ca-GP contents on the mechanical properties, i.e., Young's modulus, compressive strength, and yield strain, of hybrid composites	418:575	The effect of HAp, Na-GP, and Ca-GP contents on the mechanical properties, i.e., Young's modulus, compressive strength, and yield strain, of hybrid composites was analyzed.
33807434	5	42	from	MPa	1137:1139	arg1	strength					1067:1074	compressive strength	1055:1074	compressive strength	1055:1074	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	5	42	from	MPa	1137:1139	arg1	proliferation					1018:1030	cell proliferation	1013:1030	cell proliferation	1013:1030	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	5	42	from	MPa	1137:1139	arg1	shrinkage					1157:1165	volumetric shrinkage	1146:1165	volumetric shrinkage	1146:1165	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	5	42	from	MPa	1137:1139	arg1	modulus					1113:1119	Young's modulus	1105:1119	Young's modulus from 5.2 to 100 MPa	1105:1139	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	5	42	from	MPa	1137:1139	arg1	growth					1036:1041	growth	1036:1041	growth	1036:1041	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	5	43	theme	MPa	1100:1102	arg1	range					1081:1085	a range	1079:1085	a range of 0.3 to 10 MPa	1079:1102	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	5	44	dep	analyzed	832:839	arg1	characterized					845:857	characterized	845:857	analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%	832:1175	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	5	45	theme	pores	892:896	arg1	surface					881:887	the well-developed surface	862:887	the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%	862:1175	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	5	46	from	strength	1067:1074	arg1	range					1081:1085	a range	1079:1085	a range of 0.3 to 10 MPa	1079:1102	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	5	47	theme	compressive	1055:1065	arg1	strength					1067:1074	compressive strength	1055:1074	compressive strength	1055:1074	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	3	48	theme	materials	617:625	arg1	microstructure					595:608	The microstructure	591:608	The microstructure of the materials obtained	591:634	The microstructure of the materials obtained was visualized by SEM.
33807434	5	49	dep	many	937:940	arg1	smaller					942:948	smaller	942:948	smaller	942:948	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	2	50	theme	compressive	516:526	arg1	strength					528:535	compressive strength	516:535	compressive strength	516:535	The effect of HAp, Na-GP, and Ca-GP contents on the mechanical properties, i.e., Young's modulus, compressive strength, and yield strain, of hybrid composites was analyzed.
33807434	1	51	theme	sol-gel	402:408	arg1	method					410:415	the sol-gel method	398:415	the sol-gel method	398:415	The aim of this work was to fabricate novel bioactive composites based on chitosan and non-organic silica, reinforced with calcium β-glycerophosphate (Ca-GP), sodium β-glycerophosphate pentahydrate (Na-GP), and hydroxyapatite powder (HAp) in a range of concentrations using the sol-gel method.
33807434	4	52	theme	composites	747:756	arg1	analysis					714:721	FTIR analysis	709:721	FTIR analysis	709:721	Moreover, the molecular interactions according to FTIR analysis and biocompatibility of composites obtained were examined.
33807434	4	52	theme	composites	747:756	arg1	biocompatibility					727:742	biocompatibility	727:742	biocompatibility	727:742	Moreover, the molecular interactions according to FTIR analysis and biocompatibility of composites obtained were examined.
33807434	6	53	theme	promising	1198:1206	arg1	composite					1208:1216	a promising composite	1196:1216	a promising composite for applications in tissue engineering, e.g., filling small bone defects	1196:1289	This proved to be a promising composite for applications in tissue engineering, e.g., filling small bone defects.
33807434	6	53	theme	promising	1198:1206	arg1	This					1178:1181	This	1178:1181	This	1178:1181	This proved to be a promising composite for applications in tissue engineering, e.g., filling small bone defects.
33807434	5	54	theme	pores	950:954	arg1	behavior					973:980	the behavior	969:980	the behavior	969:980	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	1	55	theme	work	140:143	arg1	aim					128:130	The aim	124:130	The aim of this work	124:143	The aim of this work was to fabricate novel bioactive composites based on chitosan and non-organic silica, reinforced with calcium β-glycerophosphate (Ca-GP), sodium β-glycerophosphate pentahydrate (Na-GP), and hydroxyapatite powder (HAp) in a range of concentrations using the sol-gel method.
33807434	5	56	from	growth	1036:1041	arg1	range					1081:1085	a range	1079:1085	a range of 0.3 to 10 MPa	1079:1102	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	0	57	theme	Composites	84:93	arg1	Properties					39:48	Mechanical, Structural, and Biological Properties	0:48	Mechanical, Structural, and Biological Properties of Chitosan/Hydroxyapatite/Silica Composites for Bone Tissue Engineering	0:121	Mechanical, Structural, and Biological Properties of Chitosan/Hydroxyapatite/Silica Composites for Bone Tissue Engineering.
33807434	6	58	theme	bone	1278:1281	arg1	defects					1283:1289	filling small bone defects	1264:1289	filling small bone defects	1264:1289	This proved to be a promising composite for applications in tissue engineering, e.g., filling small bone defects.
33807434	0	59	theme	Chitosan/Hydroxyapatite/Silica	53:82	arg1	Composites					84:93	Chitosan/Hydroxyapatite/Silica Composites	53:93	Chitosan/Hydroxyapatite/Silica Composites	53:93	Mechanical, Structural, and Biological Properties of Chitosan/Hydroxyapatite/Silica Composites for Bone Tissue Engineering.
33807434	2	60	from	effect	422:427	arg1	properties					481:490	the mechanical properties	466:490	the mechanical properties	466:490	The effect of HAp, Na-GP, and Ca-GP contents on the mechanical properties, i.e., Young's modulus, compressive strength, and yield strain, of hybrid composites was analyzed.
33807434	5	61	dep	sizes	905:909	arg1	ones					918:921	large ones	912:921	large ones of 100 μm	912:931	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	5	61	dep	sizes	905:909	arg1	pores					950:954	many smaller pores	937:954	many smaller pores below 10 µm	937:966	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	5	62	theme	well-developed	866:879	arg1	surface					881:887	the well-developed surface	862:887	the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%	862:1175	The CS/Si/HAp/Ca-GP developed from all composites analyzed was characterized by the well-developed surface of pores of two sizes: large ones of 100 μm and many smaller pores below 10 µm, the behavior of which positively influenced cell proliferation and growth, as well as compressive strength in a range of 0.3 to 10 MPa, Young's modulus from 5.2 to 100 MPa, and volumetric shrinkage below 60%.
33807434	1	63	theme	calcium	247:253	arg1	Ca-GP					275:279	Ca-GP	275:279	Ca-GP	275:279	The aim of this work was to fabricate novel bioactive composites based on chitosan and non-organic silica, reinforced with calcium β-glycerophosphate (Ca-GP), sodium β-glycerophosphate pentahydrate (Na-GP), and hydroxyapatite powder (HAp) in a range of concentrations using the sol-gel method.
33807434	1	63	theme	calcium	247:253	arg1	β-glycerophosphate					255:272	calcium β-glycerophosphate	247:272	calcium β-glycerophosphate (Ca-GP)	247:280	The aim of this work was to fabricate novel bioactive composites based on chitosan and non-organic silica, reinforced with calcium β-glycerophosphate (Ca-GP), sodium β-glycerophosphate pentahydrate (Na-GP), and hydroxyapatite powder (HAp) in a range of concentrations using the sol-gel method.
34097963	5	0	theme	faster	796:801	arg1	transfer					803:810	faster transfer	796:810	faster transfer	796:810	These wrapping of Fe2O3 on chitosan provides synergistically improved properties that could be attributed to the elevated partition efficiency and faster transfer of the photo-generated charge carriers, which was substantiated by the experimental outcomes from photoluminescence and ESR spectroscopy.
34097963	10	1	theme	contaminated	1560:1571	arg1	water					1573:1577	contaminated water	1560:1577	contaminated water	1560:1577	This work proposed a practical arrangement and subsequent influence of an advanced photocatalyst for the useful remediation dyes from contaminated water without causing any secondary pollution.
34097963	9	2	theme	Fe2O3	1406:1410	arg1	composite					1415:1423	the fabricated Fe2O3@CS composite	1391:1423	the fabricated Fe2O3@CS composite	1391:1423	The five cycles of regeneration experiment recommended the superior photostability of the fabricated Fe2O3@CS composite.
34097963	6	3	theme	Fe2O3	1025:1029	arg1	CS					1031:1032	Fe2O3@CS	1025:1032	Fe2O3@CS (2.52 eV)	1025:1042	The results obtained from DRS analysis entail the reduction in band gap of Fe2O3@CS (2.52 eV) as compared with 3.52 eV of Fe2O3.
34097963	6	3	theme	Fe2O3	1025:1029	arg1	2.52 eV					1035:1041	2.52 eV	1035:1041	2.52 eV	1035:1041	The results obtained from DRS analysis entail the reduction in band gap of Fe2O3@CS (2.52 eV) as compared with 3.52 eV of Fe2O3.
34097963	0	4	theme	mechanistic	140:150	arg1	pathways					152:159	mechanistic pathways	140:159	mechanistic pathways	140:159	Photocatalytic performance of chitosan tethered magnetic Fe2O3-like (3D/2D) hybrid for the dynamic removal of anionic dyes: Degradation and mechanistic pathways.
34097963	9	5	theme	composite	1415:1423	arg1	photostability					1373:1386	the superior photostability	1360:1386	the superior photostability of the fabricated Fe2O3@CS composite	1360:1423	The five cycles of regeneration experiment recommended the superior photostability of the fabricated Fe2O3@CS composite.
34097963	10	6	theme	photocatalyst	1509:1521	arg1	arrangement					1457:1467	practical arrangement	1447:1467	practical arrangement	1447:1467	This work proposed a practical arrangement and subsequent influence of an advanced photocatalyst for the useful remediation dyes from contaminated water without causing any secondary pollution.
34097963	10	6	theme	photocatalyst	1509:1521	arg1	influence					1484:1492	subsequent influence	1473:1492	subsequent influence	1473:1492	This work proposed a practical arrangement and subsequent influence of an advanced photocatalyst for the useful remediation dyes from contaminated water without causing any secondary pollution.
34097963	5	7	theme	Fe2O3	667:671	arg1	wrapping					655:662	These wrapping	649:662	These wrapping of Fe2O3 on chitosan	649:683	These wrapping of Fe2O3 on chitosan provides synergistically improved properties that could be attributed to the elevated partition efficiency and faster transfer of the photo-generated charge carriers, which was substantiated by the experimental outcomes from photoluminescence and ESR spectroscopy.
34097963	10	8	from	water	1573:1577	arg1	dyes					1550:1553	the useful remediation dyes	1527:1553	the useful remediation dyes from contaminated water	1527:1577	This work proposed a practical arrangement and subsequent influence of an advanced photocatalyst for the useful remediation dyes from contaminated water without causing any secondary pollution.
34097963	10	9	theme	advanced	1500:1507	arg1	photocatalyst					1509:1521	an advanced photocatalyst	1497:1521	an advanced photocatalyst	1497:1521	This work proposed a practical arrangement and subsequent influence of an advanced photocatalyst for the useful remediation dyes from contaminated water without causing any secondary pollution.
34097963	6	10	theme	CS	1031:1032	arg1	reduction					1000:1008	the reduction	996:1008	the reduction in band gap of Fe2O3@CS (2.52 eV)	996:1042	The results obtained from DRS analysis entail the reduction in band gap of Fe2O3@CS (2.52 eV) as compared with 3.52 eV of Fe2O3.
34097963	0	11	theme	dynamic	91:97	arg1	removal					99:105	the dynamic removal	87:105	the dynamic removal of anionic dyes	87:121	Photocatalytic performance of chitosan tethered magnetic Fe2O3-like (3D/2D) hybrid for the dynamic removal of anionic dyes: Degradation and mechanistic pathways.
34097963	5	12	theme	elevated	762:769	arg1	efficiency					781:790	elevated partition efficiency	762:790	elevated partition efficiency	762:790	These wrapping of Fe2O3 on chitosan provides synergistically improved properties that could be attributed to the elevated partition efficiency and faster transfer of the photo-generated charge carriers, which was substantiated by the experimental outcomes from photoluminescence and ESR spectroscopy.
34097963	6	13	theme	@	1030:1030	arg1	CS					1031:1032	Fe2O3@CS	1025:1032	Fe2O3@CS (2.52 eV)	1025:1042	The results obtained from DRS analysis entail the reduction in band gap of Fe2O3@CS (2.52 eV) as compared with 3.52 eV of Fe2O3.
34097963	6	13	theme	@	1030:1030	arg1	2.52 eV					1035:1041	2.52 eV	1035:1041	2.52 eV	1035:1041	The results obtained from DRS analysis entail the reduction in band gap of Fe2O3@CS (2.52 eV) as compared with 3.52 eV of Fe2O3.
34097963	10	14	theme	secondary	1599:1607	arg1	pollution					1609:1617	any secondary pollution	1595:1617	any secondary pollution	1595:1617	This work proposed a practical arrangement and subsequent influence of an advanced photocatalyst for the useful remediation dyes from contaminated water without causing any secondary pollution.
34097963	4	15	theme	nanocomposite	634:646	arg1	generation					616:625	the useful generation	605:625	the useful generation of the nanocomposite	605:646	The chitosan makes available its surface for the useful generation of the nanocomposite.
34097963	10	16	theme	useful	1531:1536	arg1	dyes					1550:1553	the useful remediation dyes	1527:1553	the useful remediation dyes from contaminated water	1527:1577	This work proposed a practical arrangement and subsequent influence of an advanced photocatalyst for the useful remediation dyes from contaminated water without causing any secondary pollution.
34097963	3	17	theme	Fe2O3	490:494	arg1	CS					496:497	Fe2O3@CS	490:497	Fe2O3@CS	490:497	In this work, Fe2O3 reinforced chitosan (Fe2O3@CS) nanocomposite was fabricated using co-precipitation method.
34097963	3	17	theme	Fe2O3	490:494	arg1	chitosan					480:487	Fe2O3 reinforced chitosan	463:487	Fe2O3 reinforced chitosan (Fe2O3@CS) nanocomposite	463:512	In this work, Fe2O3 reinforced chitosan (Fe2O3@CS) nanocomposite was fabricated using co-precipitation method.
34097963	5	18	theme	charge	835:840	arg1	carriers					842:849	the photo-generated charge carriers	815:849	the photo-generated charge carriers	815:849	These wrapping of Fe2O3 on chitosan provides synergistically improved properties that could be attributed to the elevated partition efficiency and faster transfer of the photo-generated charge carriers, which was substantiated by the experimental outcomes from photoluminescence and ESR spectroscopy.
34097963	8	19	theme	CS	1290:1291	arg1	composites					1293:1302	Fe2O3@CS composites	1284:1302	Fe2O3@CS composites	1284:1302	The trapping investigation emphasized the involvement of OH radicals in the degradation of dyes over Fe2O3@CS composites.
34097963	3	20	theme	chitosan	480:487	arg1	nanocomposite					500:512	Fe2O3 reinforced chitosan (Fe2O3@CS) nanocomposite	463:512	Fe2O3 reinforced chitosan (Fe2O3@CS) nanocomposite	463:512	In this work, Fe2O3 reinforced chitosan (Fe2O3@CS) nanocomposite was fabricated using co-precipitation method.
34097963	8	21	theme	dyes	1274:1277	arg1	degradation					1259:1269	the degradation	1255:1269	the degradation of dyes	1255:1277	The trapping investigation emphasized the involvement of OH radicals in the degradation of dyes over Fe2O3@CS composites.
34097963	1	22	theme	Efficient	162:170	arg1	methods					187:193	Efficient photocatalysis methods	162:193	Efficient photocatalysis methods with a production of less number of toxic intermediates	162:249	Efficient photocatalysis methods with a production of less number of toxic intermediates are extremely advantageous for water decontamination.
34097963	5	23	theme	partition	771:779	arg1	efficiency					781:790	elevated partition efficiency	762:790	elevated partition efficiency	762:790	These wrapping of Fe2O3 on chitosan provides synergistically improved properties that could be attributed to the elevated partition efficiency and faster transfer of the photo-generated charge carriers, which was substantiated by the experimental outcomes from photoluminescence and ESR spectroscopy.
34097963	8	24	from	involvement	1225:1235	arg1	degradation					1259:1269	the degradation	1255:1269	the degradation of dyes	1255:1277	The trapping investigation emphasized the involvement of OH radicals in the degradation of dyes over Fe2O3@CS composites.
34097963	3	25	theme	co-precipitation	535:550	arg1	method					552:557	co-precipitation method	535:557	co-precipitation method	535:557	In this work, Fe2O3 reinforced chitosan (Fe2O3@CS) nanocomposite was fabricated using co-precipitation method.
34097963	1	26	theme	photocatalysis	172:185	arg1	methods					187:193	Efficient photocatalysis methods	162:193	Efficient photocatalysis methods with a production of less number of toxic intermediates	162:249	Efficient photocatalysis methods with a production of less number of toxic intermediates are extremely advantageous for water decontamination.
34097963	5	27	theme	carriers	842:849	arg1	efficiency					781:790	elevated partition efficiency	762:790	elevated partition efficiency	762:790	These wrapping of Fe2O3 on chitosan provides synergistically improved properties that could be attributed to the elevated partition efficiency and faster transfer of the photo-generated charge carriers, which was substantiated by the experimental outcomes from photoluminescence and ESR spectroscopy.
34097963	5	27	theme	carriers	842:849	arg1	transfer					803:810	faster transfer	796:810	faster transfer	796:810	These wrapping of Fe2O3 on chitosan provides synergistically improved properties that could be attributed to the elevated partition efficiency and faster transfer of the photo-generated charge carriers, which was substantiated by the experimental outcomes from photoluminescence and ESR spectroscopy.
34097963	10	28	theme	subsequent	1473:1482	arg1	influence					1484:1492	subsequent influence	1473:1492	subsequent influence	1473:1492	This work proposed a practical arrangement and subsequent influence of an advanced photocatalyst for the useful remediation dyes from contaminated water without causing any secondary pollution.
34097963	9	29	theme	regeneration	1324:1335	arg1	experiment					1337:1346	regeneration experiment	1324:1346	regeneration experiment	1324:1346	The five cycles of regeneration experiment recommended the superior photostability of the fabricated Fe2O3@CS composite.
34097963	2	30	theme	specific	333:340	arg1	area					350:353	specific surface area	333:353	specific surface area	333:353	The degradation efficiency, specific surface area, stability and porosity will be improving by wrapping of Fe2O3 using appropriate biopolymers.
34097963	0	31	theme	Photocatalytic	0:13	arg1	performance					15:25	Photocatalytic performance	0:25	Photocatalytic performance of chitosan	0:37	Photocatalytic performance of chitosan tethered magnetic Fe2O3-like (3D/2D) hybrid for the dynamic removal of anionic dyes: Degradation and mechanistic pathways.
34097963	1	32	theme	water	282:286	arg1	decontamination					288:302	water decontamination	282:302	water decontamination	282:302	Efficient photocatalysis methods with a production of less number of toxic intermediates are extremely advantageous for water decontamination.
34097963	10	33	dep	arrangement	1457:1467	arg1	a					1445:1445	a	1445:1445	a	1445:1445	This work proposed a practical arrangement and subsequent influence of an advanced photocatalyst for the useful remediation dyes from contaminated water without causing any secondary pollution.
34097963	10	34	theme	practical	1447:1455	arg1	arrangement					1457:1467	practical arrangement	1447:1467	practical arrangement	1447:1467	This work proposed a practical arrangement and subsequent influence of an advanced photocatalyst for the useful remediation dyes from contaminated water without causing any secondary pollution.
34097963	0	35	theme	chitosan	30:37	arg1	performance					15:25	Photocatalytic performance	0:25	Photocatalytic performance of chitosan	0:37	Photocatalytic performance of chitosan tethered magnetic Fe2O3-like (3D/2D) hybrid for the dynamic removal of anionic dyes: Degradation and mechanistic pathways.
34097963	5	36	from	wrapping	655:662	arg1	chitosan					676:683	chitosan	676:683	chitosan	676:683	These wrapping of Fe2O3 on chitosan provides synergistically improved properties that could be attributed to the elevated partition efficiency and faster transfer of the photo-generated charge carriers, which was substantiated by the experimental outcomes from photoluminescence and ESR spectroscopy.
34097963	0	37	theme	dyes	118:121	arg1	removal					99:105	the dynamic removal	87:105	the dynamic removal of anionic dyes	87:121	Photocatalytic performance of chitosan tethered magnetic Fe2O3-like (3D/2D) hybrid for the dynamic removal of anionic dyes: Degradation and mechanistic pathways.
34097963	5	38	from	spectroscopy	936:947	arg1	outcomes					896:903	the experimental outcomes	879:903	the experimental outcomes from photoluminescence and ESR spectroscopy	879:947	These wrapping of Fe2O3 on chitosan provides synergistically improved properties that could be attributed to the elevated partition efficiency and faster transfer of the photo-generated charge carriers, which was substantiated by the experimental outcomes from photoluminescence and ESR spectroscopy.
34097963	3	39	theme	reinforced	469:478	arg1	CS					496:497	Fe2O3@CS	490:497	Fe2O3@CS	490:497	In this work, Fe2O3 reinforced chitosan (Fe2O3@CS) nanocomposite was fabricated using co-precipitation method.
34097963	3	39	theme	reinforced	469:478	arg1	chitosan					480:487	Fe2O3 reinforced chitosan	463:487	Fe2O3 reinforced chitosan (Fe2O3@CS) nanocomposite	463:512	In this work, Fe2O3 reinforced chitosan (Fe2O3@CS) nanocomposite was fabricated using co-precipitation method.
34097963	6	40	theme	band	1013:1016	arg1	gap					1018:1020	band gap	1013:1020	band gap	1013:1020	The results obtained from DRS analysis entail the reduction in band gap of Fe2O3@CS (2.52 eV) as compared with 3.52 eV of Fe2O3.
34097963	0	41	theme	anionic	110:116	arg1	dyes					118:121	anionic dyes	110:121	anionic dyes	110:121	Photocatalytic performance of chitosan tethered magnetic Fe2O3-like (3D/2D) hybrid for the dynamic removal of anionic dyes: Degradation and mechanistic pathways.
34097963	2	42	theme	degradation	309:319	arg1	efficiency					321:330	degradation efficiency	309:330	degradation efficiency	309:330	The degradation efficiency, specific surface area, stability and porosity will be improving by wrapping of Fe2O3 using appropriate biopolymers.
34097963	2	43	theme	appropriate	424:434	arg1	biopolymers					436:446	appropriate biopolymers	424:446	appropriate biopolymers	424:446	The degradation efficiency, specific surface area, stability and porosity will be improving by wrapping of Fe2O3 using appropriate biopolymers.
34097963	0	44	theme	magnetic	48:55	arg1	hybrid					76:81	magnetic Fe2O3-like (3D/2D) hybrid	48:81	magnetic Fe2O3-like (3D/2D) hybrid for the dynamic removal of anionic dyes	48:121	Photocatalytic performance of chitosan tethered magnetic Fe2O3-like (3D/2D) hybrid for the dynamic removal of anionic dyes: Degradation and mechanistic pathways.
34097963	5	45	dep	efficiency	781:790	arg1	the					758:760	the	758:760	the	758:760	These wrapping of Fe2O3 on chitosan provides synergistically improved properties that could be attributed to the elevated partition efficiency and faster transfer of the photo-generated charge carriers, which was substantiated by the experimental outcomes from photoluminescence and ESR spectroscopy.
34097963	8	46	theme	Fe2O3	1284:1288	arg1	composites					1293:1302	Fe2O3@CS composites	1284:1302	Fe2O3@CS composites	1284:1302	The trapping investigation emphasized the involvement of OH radicals in the degradation of dyes over Fe2O3@CS composites.
34097963	7	47	theme	maximum	1131:1137	arg1	%					1110:1110	89.2%	1106:1110	89.2%	1106:1110	The results indicated that 89.2% and 94.6% were the maximum degradations correspondingly for MO and OG.
34097963	7	47	theme	maximum	1131:1137	arg1	%					1120:1120	94.6%	1116:1120	94.6%	1116:1120	The results indicated that 89.2% and 94.6% were the maximum degradations correspondingly for MO and OG.
34097963	7	47	theme	maximum	1131:1137	arg1	degradations					1139:1150	the maximum degradations	1127:1150	the maximum degradations	1127:1150	The results indicated that 89.2% and 94.6% were the maximum degradations correspondingly for MO and OG.
34097963	9	48	theme	superior	1364:1371	arg1	photostability					1373:1386	the superior photostability	1360:1386	the superior photostability of the fabricated Fe2O3@CS composite	1360:1423	The five cycles of regeneration experiment recommended the superior photostability of the fabricated Fe2O3@CS composite.
34097963	9	49	theme	@	1411:1411	arg1	composite					1415:1423	the fabricated Fe2O3@CS composite	1391:1423	the fabricated Fe2O3@CS composite	1391:1423	The five cycles of regeneration experiment recommended the superior photostability of the fabricated Fe2O3@CS composite.
34097963	5	50	theme	experimental	883:894	arg1	outcomes					896:903	the experimental outcomes	879:903	the experimental outcomes from photoluminescence and ESR spectroscopy	879:947	These wrapping of Fe2O3 on chitosan provides synergistically improved properties that could be attributed to the elevated partition efficiency and faster transfer of the photo-generated charge carriers, which was substantiated by the experimental outcomes from photoluminescence and ESR spectroscopy.
34097963	2	51	theme	Fe2O3	412:416	arg1	wrapping					400:407	wrapping	400:407	wrapping of Fe2O3 using appropriate biopolymers	400:446	The degradation efficiency, specific surface area, stability and porosity will be improving by wrapping of Fe2O3 using appropriate biopolymers.
34097963	5	52	theme	photo-generated	819:833	arg1	carriers					842:849	the photo-generated charge carriers	815:849	the photo-generated charge carriers	815:849	These wrapping of Fe2O3 on chitosan provides synergistically improved properties that could be attributed to the elevated partition efficiency and faster transfer of the photo-generated charge carriers, which was substantiated by the experimental outcomes from photoluminescence and ESR spectroscopy.
34097963	1	53	with	methods	187:193	arg1	production					202:211	a production	200:211	a production of less number of toxic intermediates	200:249	Efficient photocatalysis methods with a production of less number of toxic intermediates are extremely advantageous for water decontamination.
34097963	9	54	theme	experiment	1337:1346	arg1	cycles					1314:1319	The five cycles	1305:1319	The five cycles of regeneration experiment	1305:1346	The five cycles of regeneration experiment recommended the superior photostability of the fabricated Fe2O3@CS composite.
34097963	6	55	from	reduction	1000:1008	arg1	gap					1018:1020	band gap	1013:1020	band gap	1013:1020	The results obtained from DRS analysis entail the reduction in band gap of Fe2O3@CS (2.52 eV) as compared with 3.52 eV of Fe2O3.
34097963	3	56	theme	@	495:495	arg1	CS					496:497	Fe2O3@CS	490:497	Fe2O3@CS	490:497	In this work, Fe2O3 reinforced chitosan (Fe2O3@CS) nanocomposite was fabricated using co-precipitation method.
34097963	3	56	theme	@	495:495	arg1	chitosan					480:487	Fe2O3 reinforced chitosan	463:487	Fe2O3 reinforced chitosan (Fe2O3@CS) nanocomposite	463:512	In this work, Fe2O3 reinforced chitosan (Fe2O3@CS) nanocomposite was fabricated using co-precipitation method.
34097963	0	57	theme	Fe2O3-like	57:66	arg1	hybrid					76:81	magnetic Fe2O3-like (3D/2D) hybrid	48:81	magnetic Fe2O3-like (3D/2D) hybrid for the dynamic removal of anionic dyes	48:121	Photocatalytic performance of chitosan tethered magnetic Fe2O3-like (3D/2D) hybrid for the dynamic removal of anionic dyes: Degradation and mechanistic pathways.
34097963	10	58	theme	remediation	1538:1548	arg1	dyes					1550:1553	the useful remediation dyes	1527:1553	the useful remediation dyes from contaminated water	1527:1577	This work proposed a practical arrangement and subsequent influence of an advanced photocatalyst for the useful remediation dyes from contaminated water without causing any secondary pollution.
34097963	8	59	theme	@	1289:1289	arg1	composites					1293:1302	Fe2O3@CS composites	1284:1302	Fe2O3@CS composites	1284:1302	The trapping investigation emphasized the involvement of OH radicals in the degradation of dyes over Fe2O3@CS composites.
34097963	5	60	theme	improved	710:717	arg1	properties					719:728	synergistically improved properties	694:728	synergistically improved properties that could be attributed to the elevated partition efficiency and faster transfer of the photo-generated charge carriers, which was substantiated by the experimental outcomes from photoluminescence and ESR spectroscopy	694:947	These wrapping of Fe2O3 on chitosan provides synergistically improved properties that could be attributed to the elevated partition efficiency and faster transfer of the photo-generated charge carriers, which was substantiated by the experimental outcomes from photoluminescence and ESR spectroscopy.
34097963	9	61	theme	fabricated	1395:1404	arg1	composite					1415:1423	the fabricated Fe2O3@CS composite	1391:1423	the fabricated Fe2O3@CS composite	1391:1423	The five cycles of regeneration experiment recommended the superior photostability of the fabricated Fe2O3@CS composite.
34097963	1	62	theme	number	221:226	arg1	production					202:211	a production	200:211	a production of less number of toxic intermediates	200:249	Efficient photocatalysis methods with a production of less number of toxic intermediates are extremely advantageous for water decontamination.
34097963	5	63	from	photoluminescence	910:926	arg1	outcomes					896:903	the experimental outcomes	879:903	the experimental outcomes from photoluminescence and ESR spectroscopy	879:947	These wrapping of Fe2O3 on chitosan provides synergistically improved properties that could be attributed to the elevated partition efficiency and faster transfer of the photo-generated charge carriers, which was substantiated by the experimental outcomes from photoluminescence and ESR spectroscopy.
34097963	2	64	theme	surface	342:348	arg1	area					350:353	specific surface area	333:353	specific surface area	333:353	The degradation efficiency, specific surface area, stability and porosity will be improving by wrapping of Fe2O3 using appropriate biopolymers.
34097963	8	65	theme	OH	1240:1241	arg1	radicals					1243:1250	OH radicals	1240:1250	OH radicals	1240:1250	The trapping investigation emphasized the involvement of OH radicals in the degradation of dyes over Fe2O3@CS composites.
34097963	4	66	theme	useful	609:614	arg1	generation					616:625	the useful generation	605:625	the useful generation of the nanocomposite	605:646	The chitosan makes available its surface for the useful generation of the nanocomposite.
34097963	3	67	theme	Fe2O3	463:467	arg1	CS					496:497	Fe2O3@CS	490:497	Fe2O3@CS	490:497	In this work, Fe2O3 reinforced chitosan (Fe2O3@CS) nanocomposite was fabricated using co-precipitation method.
34097963	3	67	theme	Fe2O3	463:467	arg1	chitosan					480:487	Fe2O3 reinforced chitosan	463:487	Fe2O3 reinforced chitosan (Fe2O3@CS) nanocomposite	463:512	In this work, Fe2O3 reinforced chitosan (Fe2O3@CS) nanocomposite was fabricated using co-precipitation method.
34097963	6	68	theme	Fe2O3	1072:1076	arg1	3.52 eV					1061:1067	3.52 eV	1061:1067	3.52 eV of Fe2O3	1061:1076	The results obtained from DRS analysis entail the reduction in band gap of Fe2O3@CS (2.52 eV) as compared with 3.52 eV of Fe2O3.
34097963	5	69	theme	ESR	932:934	arg1	spectroscopy					936:947	ESR spectroscopy	932:947	ESR spectroscopy	932:947	These wrapping of Fe2O3 on chitosan provides synergistically improved properties that could be attributed to the elevated partition efficiency and faster transfer of the photo-generated charge carriers, which was substantiated by the experimental outcomes from photoluminescence and ESR spectroscopy.
34097963	8	70	theme	radicals	1243:1250	arg1	involvement					1225:1235	the involvement	1221:1235	the involvement of OH radicals in the degradation of dyes	1221:1277	The trapping investigation emphasized the involvement of OH radicals in the degradation of dyes over Fe2O3@CS composites.
34097963	1	71	theme	toxic	231:235	arg1	intermediates					237:249	toxic intermediates	231:249	toxic intermediates	231:249	Efficient photocatalysis methods with a production of less number of toxic intermediates are extremely advantageous for water decontamination.
34097963	0	72	theme	3D/2D	69:73	arg1	hybrid					76:81	magnetic Fe2O3-like (3D/2D) hybrid	48:81	magnetic Fe2O3-like (3D/2D) hybrid for the dynamic removal of anionic dyes	48:121	Photocatalytic performance of chitosan tethered magnetic Fe2O3-like (3D/2D) hybrid for the dynamic removal of anionic dyes: Degradation and mechanistic pathways.
34097963	6	73	theme	DRS	976:978	arg1	analysis					980:987	DRS analysis	976:987	DRS analysis	976:987	The results obtained from DRS analysis entail the reduction in band gap of Fe2O3@CS (2.52 eV) as compared with 3.52 eV of Fe2O3.
34097963	9	74	theme	CS	1412:1413	arg1	composite					1415:1423	the fabricated Fe2O3@CS composite	1391:1423	the fabricated Fe2O3@CS composite	1391:1423	The five cycles of regeneration experiment recommended the superior photostability of the fabricated Fe2O3@CS composite.
34097963	8	75	theme	trapping	1187:1194	arg1	investigation					1196:1208	The trapping investigation	1183:1208	The trapping investigation	1183:1208	The trapping investigation emphasized the involvement of OH radicals in the degradation of dyes over Fe2O3@CS composites.
34097963	1	76	theme	intermediates	237:249	arg1	number					221:226	less number	216:226	less number of toxic intermediates	216:249	Efficient photocatalysis methods with a production of less number of toxic intermediates are extremely advantageous for water decontamination.
33799246	0	0	theme	starch	107:112	arg1	particles					124:132	dihydromyricetin/high-amylose corn starch composite particles	72:132	dihydromyricetin/high-amylose corn starch composite particles	72:132	Multi-scale stabilization mechanism of pickering emulsion gels based on dihydromyricetin/high-amylose corn starch composite particles.
33799246	4	1	theme	ionic	708:712	arg1	strength					714:721	ionic strength	708:721	ionic strength	708:721	The obtained emulsion gels were not sensitive to ionic strength, which could be attributed to emulsifying capacity and viscosity effect of composite particles.
33799246	6	2	theme	interfacial	1100:1110	arg1	tension					1112:1118	the interfacial tension	1096:1118	the interfacial tension	1096:1118	The molecular simulation of the supramolecular complexes in the oil-water system indicated that these complexes could spontaneously aggregate and anchor to the oil-water interface, reducing the interfacial tension.
33799246	3	3	theme	composite	508:516	arg1	particles					518:526	composite particles	508:526	composite particles	508:526	The DMY content in composite particles and the amount of addition of composite particles had obvious synergistic effect on the formation and properties of emulsion gels.
33799246	0	4	theme	corn	102:105	arg1	particles					124:132	dihydromyricetin/high-amylose corn starch composite particles	72:132	dihydromyricetin/high-amylose corn starch composite particles	72:132	Multi-scale stabilization mechanism of pickering emulsion gels based on dihydromyricetin/high-amylose corn starch composite particles.
33799246	6	5	theme	complexes	953:961	arg1	simulation					920:929	The molecular simulation	906:929	The molecular simulation of the supramolecular complexes in the oil-water system	906:985	The molecular simulation of the supramolecular complexes in the oil-water system indicated that these complexes could spontaneously aggregate and anchor to the oil-water interface, reducing the interfacial tension.
33799246	1	6	theme	eco-unfriendly	277:290	arg1	pretreatments					201:213	the pretreatments	197:213	the pretreatments of acid hydrolysis, esterification, which are complicated and eco-unfriendly	197:290	For Pickering emulsifying effect, starch must be subjected to the pretreatments of acid hydrolysis, esterification, which are complicated and eco-unfriendly.
33799246	6	7	from	simulation	920:929	arg1	system					980:985	the oil-water system	966:985	the oil-water system	966:985	The molecular simulation of the supramolecular complexes in the oil-water system indicated that these complexes could spontaneously aggregate and anchor to the oil-water interface, reducing the interfacial tension.
33799246	6	8	theme	supramolecular	938:951	arg1	complexes					953:961	the supramolecular complexes	934:961	the supramolecular complexes	934:961	The molecular simulation of the supramolecular complexes in the oil-water system indicated that these complexes could spontaneously aggregate and anchor to the oil-water interface, reducing the interfacial tension.
33799246	3	9	theme	addition	546:553	arg1	content					497:503	The DMY content	489:503	The DMY content in composite particles	489:526	The DMY content in composite particles and the amount of addition of composite particles had obvious synergistic effect on the formation and properties of emulsion gels.
33799246	3	9	theme	addition	546:553	arg1	addition					546:553	addition	546:553	addition of composite particles	546:576	The DMY content in composite particles and the amount of addition of composite particles had obvious synergistic effect on the formation and properties of emulsion gels.
33799246	3	9	theme	addition	546:553	arg1	amount					536:541	the amount	532:541	the amount of addition of composite particles	532:576	The DMY content in composite particles and the amount of addition of composite particles had obvious synergistic effect on the formation and properties of emulsion gels.
33799246	3	10	theme	particles	568:576	arg1	addition					546:553	addition	546:553	addition of composite particles	546:576	The DMY content in composite particles and the amount of addition of composite particles had obvious synergistic effect on the formation and properties of emulsion gels.
33799246	0	11	theme	composite	114:122	arg1	particles					124:132	dihydromyricetin/high-amylose corn starch composite particles	72:132	dihydromyricetin/high-amylose corn starch composite particles	72:132	Multi-scale stabilization mechanism of pickering emulsion gels based on dihydromyricetin/high-amylose corn starch composite particles.
33799246	7	12	theme	multi-scale	1172:1182	arg1	relationship					1184:1195	the multi-scale relationship	1168:1195	the multi-scale relationship of "molecular interaction-particle characteristics-gel properties"	1168:1262	Based on experimental and theoretical results, the multi-scale relationship of "molecular interaction-particle characteristics-gel properties" was established.
33799246	4	13	theme	capacity	765:772	arg1	effect					788:793	emulsifying capacity and viscosity effect	753:793	emulsifying capacity and viscosity effect of composite particles	753:816	The obtained emulsion gels were not sensitive to ionic strength, which could be attributed to emulsifying capacity and viscosity effect of composite particles.
33799246	5	14	theme	host-guest	879:888	arg1	supramolecules					890:903	DMY/amylose host-guest supramolecules	867:903	DMY/amylose host-guest supramolecules	867:903	The spectral analysis confirmed the presence of DMY/amylose host-guest supramolecules.
33799246	5	15	theme	supramolecules	890:903	arg1	presence					855:862	the presence	851:862	the presence of DMY/amylose host-guest supramolecules	851:903	The spectral analysis confirmed the presence of DMY/amylose host-guest supramolecules.
33799246	2	16	theme	starch	421:426	arg1	particles					444:452	dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles	380:452	dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles	380:452	In this study, a practical and green strategyto fabricate Pickering emulsion gels with dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles was introduced for the first time.
33799246	3	17	dep	formation	616:624	arg1	the					612:614	the	612:614	the	612:614	The DMY content in composite particles and the amount of addition of composite particles had obvious synergistic effect on the formation and properties of emulsion gels.
33799246	7	18	theme	interaction-particle	1211:1230	arg1	properties					1252:1261	molecular interaction-particle characteristics-gel properties	1201:1261	"molecular interaction-particle characteristics-gel properties"	1200:1262	Based on experimental and theoretical results, the multi-scale relationship of "molecular interaction-particle characteristics-gel properties" was established.
33799246	4	19	theme	viscosity	778:786	arg1	effect					788:793	emulsifying capacity and viscosity effect	753:793	emulsifying capacity and viscosity effect of composite particles	753:816	The obtained emulsion gels were not sensitive to ionic strength, which could be attributed to emulsifying capacity and viscosity effect of composite particles.
33799246	2	20	theme	corn	416:419	arg1	particles					444:452	dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles	380:452	dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles	380:452	In this study, a practical and green strategyto fabricate Pickering emulsion gels with dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles was introduced for the first time.
33799246	0	21	theme	stabilization	12:24	arg1	mechanism					26:34	Multi-scale stabilization mechanism	0:34	Multi-scale stabilization mechanism of pickering emulsion gels	0:61	Multi-scale stabilization mechanism of pickering emulsion gels based on dihydromyricetin/high-amylose corn starch composite particles.
33799246	3	22	contain	had	578:580	arg1	content					497:503	The DMY content	489:503	The DMY content in composite particles	489:526	The DMY content in composite particles and the amount of addition of composite particles had obvious synergistic effect on the formation and properties of emulsion gels.
33799246	3	22	contain	had	578:580	arg1	addition					546:553	addition	546:553	addition of composite particles	546:576	The DMY content in composite particles and the amount of addition of composite particles had obvious synergistic effect on the formation and properties of emulsion gels.
33799246	3	22	contain	had	578:580	arg1	amount					536:541	the amount	532:541	the amount of addition of composite particles	532:576	The DMY content in composite particles and the amount of addition of composite particles had obvious synergistic effect on the formation and properties of emulsion gels.
33799246	3	22	contain	had	578:580	arg2	effect					602:607	obvious synergistic effect	582:607	obvious synergistic effect	582:607	The DMY content in composite particles and the amount of addition of composite particles had obvious synergistic effect on the formation and properties of emulsion gels.
33799246	4	23	theme	obtained	663:670	arg1	gels					681:684	The obtained emulsion gels	659:684	The obtained emulsion gels	659:684	The obtained emulsion gels were not sensitive to ionic strength, which could be attributed to emulsifying capacity and viscosity effect of composite particles.
33799246	4	23	theme	obtained	663:670	arg1	sensitive					695:703	sensitive	695:703	sensitive	695:703	The obtained emulsion gels were not sensitive to ionic strength, which could be attributed to emulsifying capacity and viscosity effect of composite particles.
33799246	2	24	theme	/high-amylose	402:414	arg1	particles					444:452	dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles	380:452	dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles	380:452	In this study, a practical and green strategyto fabricate Pickering emulsion gels with dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles was introduced for the first time.
33799246	0	25	theme	Multi-scale	0:10	arg1	mechanism					26:34	Multi-scale stabilization mechanism	0:34	Multi-scale stabilization mechanism of pickering emulsion gels	0:61	Multi-scale stabilization mechanism of pickering emulsion gels based on dihydromyricetin/high-amylose corn starch composite particles.
33799246	7	26	theme	theoretical	1147:1157	arg1	results					1159:1165	experimental and theoretical results	1130:1165	experimental and theoretical results	1130:1165	Based on experimental and theoretical results, the multi-scale relationship of "molecular interaction-particle characteristics-gel properties" was established.
33799246	3	27	from	content	497:503	arg1	particles					518:526	composite particles	508:526	composite particles	508:526	The DMY content in composite particles and the amount of addition of composite particles had obvious synergistic effect on the formation and properties of emulsion gels.
33799246	2	28	theme	green	324:328	arg1	strategyto					330:339	a practical and green strategyto	308:339	a practical and green strategyto fabricate Pickering emulsion gels with dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles	308:452	In this study, a practical and green strategyto fabricate Pickering emulsion gels with dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles was introduced for the first time.
33799246	7	29	theme	properties	1252:1261	arg1	"					1262:1262	"molecular interaction-particle characteristics-gel properties"	1200:1262	"molecular interaction-particle characteristics-gel properties"	1200:1262	Based on experimental and theoretical results, the multi-scale relationship of "molecular interaction-particle characteristics-gel properties" was established.
33799246	5	30	theme	spectral	823:830	arg1	analysis					832:839	The spectral analysis	819:839	The spectral analysis	819:839	The spectral analysis confirmed the presence of DMY/amylose host-guest supramolecules.
33799246	3	31	theme	composite	558:566	arg1	particles					568:576	composite particles	558:576	composite particles	558:576	The DMY content in composite particles and the amount of addition of composite particles had obvious synergistic effect on the formation and properties of emulsion gels.
33799246	1	32	theme	acid	218:221	arg1	hydrolysis					223:232	acid hydrolysis	218:232	acid hydrolysis	218:232	For Pickering emulsifying effect, starch must be subjected to the pretreatments of acid hydrolysis, esterification, which are complicated and eco-unfriendly.
33799246	3	33	theme	obvious	582:588	arg1	effect					602:607	obvious synergistic effect	582:607	obvious synergistic effect	582:607	The DMY content in composite particles and the amount of addition of composite particles had obvious synergistic effect on the formation and properties of emulsion gels.
33799246	7	34	theme	experimental	1130:1141	arg1	results					1159:1165	experimental and theoretical results	1130:1165	experimental and theoretical results	1130:1165	Based on experimental and theoretical results, the multi-scale relationship of "molecular interaction-particle characteristics-gel properties" was established.
33799246	2	35	theme	practical	310:318	arg1	strategyto					330:339	a practical and green strategyto	308:339	a practical and green strategyto fabricate Pickering emulsion gels with dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles	308:452	In this study, a practical and green strategyto fabricate Pickering emulsion gels with dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles was introduced for the first time.
33799246	3	36	theme	emulsion	644:651	arg1	gels					653:656	emulsion gels	644:656	emulsion gels	644:656	The DMY content in composite particles and the amount of addition of composite particles had obvious synergistic effect on the formation and properties of emulsion gels.
33799246	1	37	theme	hydrolysis	223:232	arg1	pretreatments					201:213	the pretreatments	197:213	the pretreatments of acid hydrolysis, esterification, which are complicated and eco-unfriendly	197:290	For Pickering emulsifying effect, starch must be subjected to the pretreatments of acid hydrolysis, esterification, which are complicated and eco-unfriendly.
33799246	0	38	theme	emulsion	49:56	arg1	gels					58:61	pickering emulsion gels	39:61	pickering emulsion gels	39:61	Multi-scale stabilization mechanism of pickering emulsion gels based on dihydromyricetin/high-amylose corn starch composite particles.
33799246	2	39	theme	emulsion	361:368	arg1	gels					370:373	Pickering emulsion gels	351:373	Pickering emulsion gels with dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles	351:452	In this study, a practical and green strategyto fabricate Pickering emulsion gels with dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles was introduced for the first time.
33799246	2	40	theme	HCS	429:431	arg1	particles					444:452	dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles	380:452	dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles	380:452	In this study, a practical and green strategyto fabricate Pickering emulsion gels with dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles was introduced for the first time.
33799246	0	41	theme	pickering	39:47	arg1	gels					58:61	pickering emulsion gels	39:61	pickering emulsion gels	39:61	Multi-scale stabilization mechanism of pickering emulsion gels based on dihydromyricetin/high-amylose corn starch composite particles.
33799246	7	42	theme	characteristics-gel	1232:1250	arg1	properties					1252:1261	molecular interaction-particle characteristics-gel properties	1201:1261	"molecular interaction-particle characteristics-gel properties"	1200:1262	Based on experimental and theoretical results, the multi-scale relationship of "molecular interaction-particle characteristics-gel properties" was established.
33799246	2	43	with	gels	370:373	arg1	particles					444:452	dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles	380:452	dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles	380:452	In this study, a practical and green strategyto fabricate Pickering emulsion gels with dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles was introduced for the first time.
33799246	1	44	theme	esterification	235:248	arg1	pretreatments					201:213	the pretreatments	197:213	the pretreatments of acid hydrolysis, esterification, which are complicated and eco-unfriendly	197:290	For Pickering emulsifying effect, starch must be subjected to the pretreatments of acid hydrolysis, esterification, which are complicated and eco-unfriendly.
33799246	2	45	theme	first	477:481	arg1	time					483:486	the first time	473:486	the first time	473:486	In this study, a practical and green strategyto fabricate Pickering emulsion gels with dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles was introduced for the first time.
33799246	3	46	theme	gels	653:656	arg1	properties					630:639	properties	630:639	properties	630:639	The DMY content in composite particles and the amount of addition of composite particles had obvious synergistic effect on the formation and properties of emulsion gels.
33799246	3	46	theme	gels	653:656	arg1	formation					616:624	formation	616:624	formation	616:624	The DMY content in composite particles and the amount of addition of composite particles had obvious synergistic effect on the formation and properties of emulsion gels.
33799246	3	47	theme	synergistic	590:600	arg1	effect					602:607	obvious synergistic effect	582:607	obvious synergistic effect	582:607	The DMY content in composite particles and the amount of addition of composite particles had obvious synergistic effect on the formation and properties of emulsion gels.
33799246	0	48	theme	gels	58:61	arg1	mechanism					26:34	Multi-scale stabilization mechanism	0:34	Multi-scale stabilization mechanism of pickering emulsion gels	0:61	Multi-scale stabilization mechanism of pickering emulsion gels based on dihydromyricetin/high-amylose corn starch composite particles.
33799246	1	49	theme	emulsifying	149:159	arg1	effect					161:166	emulsifying effect	149:166	emulsifying effect	149:166	For Pickering emulsifying effect, starch must be subjected to the pretreatments of acid hydrolysis, esterification, which are complicated and eco-unfriendly.
33799246	6	50	theme	oil-water	970:978	arg1	system					980:985	the oil-water system	966:985	the oil-water system	966:985	The molecular simulation of the supramolecular complexes in the oil-water system indicated that these complexes could spontaneously aggregate and anchor to the oil-water interface, reducing the interfacial tension.
33799246	7	51	theme	molecular	1201:1209	arg1	properties					1252:1261	molecular interaction-particle characteristics-gel properties	1201:1261	"molecular interaction-particle characteristics-gel properties"	1200:1262	Based on experimental and theoretical results, the multi-scale relationship of "molecular interaction-particle characteristics-gel properties" was established.
33799246	6	52	theme	molecular	910:918	arg1	simulation					920:929	The molecular simulation	906:929	The molecular simulation of the supramolecular complexes in the oil-water system	906:985	The molecular simulation of the supramolecular complexes in the oil-water system indicated that these complexes could spontaneously aggregate and anchor to the oil-water interface, reducing the interfacial tension.
33799246	4	53	theme	composite	798:806	arg1	particles					808:816	composite particles	798:816	composite particles	798:816	The obtained emulsion gels were not sensitive to ionic strength, which could be attributed to emulsifying capacity and viscosity effect of composite particles.
33799246	2	54	theme	Pickering	351:359	arg1	gels					370:373	Pickering emulsion gels	351:373	Pickering emulsion gels with dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles	351:452	In this study, a practical and green strategyto fabricate Pickering emulsion gels with dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles was introduced for the first time.
33799246	4	55	theme	emulsifying	753:763	arg1	effect					788:793	emulsifying capacity and viscosity effect	753:793	emulsifying capacity and viscosity effect of composite particles	753:816	The obtained emulsion gels were not sensitive to ionic strength, which could be attributed to emulsifying capacity and viscosity effect of composite particles.
33799246	3	56	theme	DMY	493:495	arg1	content					497:503	The DMY content	489:503	The DMY content in composite particles	489:526	The DMY content in composite particles and the amount of addition of composite particles had obvious synergistic effect on the formation and properties of emulsion gels.
33799246	2	57	dep	strategyto	330:339	arg1	fabricate					341:349	fabricate	341:349	fabricate Pickering emulsion gels with dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles	341:452	In this study, a practical and green strategyto fabricate Pickering emulsion gels with dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles was introduced for the first time.
33799246	0	58	theme	dihydromyricetin/high-amylose	72:100	arg1	particles					124:132	dihydromyricetin/high-amylose corn starch composite particles	72:132	dihydromyricetin/high-amylose corn starch composite particles	72:132	Multi-scale stabilization mechanism of pickering emulsion gels based on dihydromyricetin/high-amylose corn starch composite particles.
33799246	5	59	theme	DMY/amylose	867:877	arg1	supramolecules					890:903	DMY/amylose host-guest supramolecules	867:903	DMY/amylose host-guest supramolecules	867:903	The spectral analysis confirmed the presence of DMY/amylose host-guest supramolecules.
33799246	2	60	theme	composite	434:442	arg1	particles					444:452	dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles	380:452	dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles	380:452	In this study, a practical and green strategyto fabricate Pickering emulsion gels with dihydromyricetin (DMY)/high-amylose corn starch (HCS) composite particles was introduced for the first time.
33799246	4	61	theme	emulsion	672:679	arg1	gels					681:684	The obtained emulsion gels	659:684	The obtained emulsion gels	659:684	The obtained emulsion gels were not sensitive to ionic strength, which could be attributed to emulsifying capacity and viscosity effect of composite particles.
33799246	4	61	theme	emulsion	672:679	arg1	sensitive					695:703	sensitive	695:703	sensitive	695:703	The obtained emulsion gels were not sensitive to ionic strength, which could be attributed to emulsifying capacity and viscosity effect of composite particles.
33799246	6	62	theme	oil-water	1066:1074	arg1	interface					1076:1084	the oil-water interface	1062:1084	the oil-water interface	1062:1084	The molecular simulation of the supramolecular complexes in the oil-water system indicated that these complexes could spontaneously aggregate and anchor to the oil-water interface, reducing the interfacial tension.
33799246	4	63	theme	particles	808:816	arg1	effect					788:793	emulsifying capacity and viscosity effect	753:793	emulsifying capacity and viscosity effect of composite particles	753:816	The obtained emulsion gels were not sensitive to ionic strength, which could be attributed to emulsifying capacity and viscosity effect of composite particles.
33799246	7	64	theme	"	1262:1262	arg1	relationship					1184:1195	the multi-scale relationship	1168:1195	the multi-scale relationship of "molecular interaction-particle characteristics-gel properties"	1168:1262	Based on experimental and theoretical results, the multi-scale relationship of "molecular interaction-particle characteristics-gel properties" was established.
33799246	3	65	from	amount	536:541	arg1	particles					518:526	composite particles	508:526	composite particles	508:526	The DMY content in composite particles and the amount of addition of composite particles had obvious synergistic effect on the formation and properties of emulsion gels.
33387758	0	0	theme	carboxymethyl	82:94	arg1	film					116:119	acid/ sodium carboxymethyl cellulose composite film	69:119	acid/ sodium carboxymethyl cellulose composite film	69:119	Effects of ultrasonication on the properties of maize starch/stearic acid/ sodium carboxymethyl cellulose composite film.
33387758	0	1	theme	sodium	75:80	arg1	film					116:119	acid/ sodium carboxymethyl cellulose composite film	69:119	acid/ sodium carboxymethyl cellulose composite film	69:119	Effects of ultrasonication on the properties of maize starch/stearic acid/ sodium carboxymethyl cellulose composite film.
33387758	7	2	theme	composite	985:993	arg1	film					995:998	The composite film	981:998	The composite film with ultrasonication	981:1019	The composite film with ultrasonication exhibited better tensile and moisture barrier properties than the nonsonicated film.
33387758	2	3	theme	film	374:377	arg1	transmittance					241:253	The light transmittance	231:253	The light transmittance	231:253	The light transmittance, crystalline structure, microstructure, surface morphology, moisture barrier, and mechanical properties of a composite film with or without ultrasonication were investigated.
33387758	2	3	theme	film	374:377	arg1	morphology					303:312	surface morphology	295:312	surface morphology	295:312	The light transmittance, crystalline structure, microstructure, surface morphology, moisture barrier, and mechanical properties of a composite film with or without ultrasonication were investigated.
33387758	2	3	theme	film	374:377	arg1	properties					348:357	mechanical properties	337:357	mechanical properties	337:357	The light transmittance, crystalline structure, microstructure, surface morphology, moisture barrier, and mechanical properties of a composite film with or without ultrasonication were investigated.
33387758	2	3	theme	film	374:377	arg1	structure					268:276	crystalline structure	256:276	crystalline structure	256:276	The light transmittance, crystalline structure, microstructure, surface morphology, moisture barrier, and mechanical properties of a composite film with or without ultrasonication were investigated.
33387758	2	3	theme	film	374:377	arg1	microstructure					279:292	microstructure	279:292	microstructure	279:292	The light transmittance, crystalline structure, microstructure, surface morphology, moisture barrier, and mechanical properties of a composite film with or without ultrasonication were investigated.
33387758	2	3	theme	film	374:377	arg1	barrier					324:330	moisture barrier	315:330	moisture barrier	315:330	The light transmittance, crystalline structure, microstructure, surface morphology, moisture barrier, and mechanical properties of a composite film with or without ultrasonication were investigated.
33387758	0	4	theme	composite	106:114	arg1	film					116:119	acid/ sodium carboxymethyl cellulose composite film	69:119	acid/ sodium carboxymethyl cellulose composite film	69:119	Effects of ultrasonication on the properties of maize starch/stearic acid/ sodium carboxymethyl cellulose composite film.
33387758	0	5	from	Effects	0:6	arg1	properties					34:43	the properties	30:43	the properties of maize	30:52	Effects of ultrasonication on the properties of maize starch/stearic acid/ sodium carboxymethyl cellulose composite film.
33387758	2	6	theme	moisture	315:322	arg1	barrier					324:330	moisture barrier	315:330	moisture barrier	315:330	The light transmittance, crystalline structure, microstructure, surface morphology, moisture barrier, and mechanical properties of a composite film with or without ultrasonication were investigated.
33387758	4	7	theme	V-type	622:627	arg1	formation					637:645	V-type complex formation	622:645	V-type complex formation	622:645	Ultrasonication did not change the crystalline structure of the polymer film, but promoted V-type complex formation.
33387758	0	8	theme	cellulose	96:104	arg1	film					116:119	acid/ sodium carboxymethyl cellulose composite film	69:119	acid/ sodium carboxymethyl cellulose composite film	69:119	Effects of ultrasonication on the properties of maize starch/stearic acid/ sodium carboxymethyl cellulose composite film.
33387758	3	9	contain	has	508:510	arg2	transparency					517:528	good transparency	512:528	good transparency	512:528	Ultrasound increases the film's light transmittance, resulting in a film that has good transparency.
33387758	3	9	contain	has	508:510	arg1	film					498:501	a film	496:501	a film that has good transparency	496:528	Ultrasound increases the film's light transmittance, resulting in a film that has good transparency.
33387758	8	10	theme	long-term	1115:1123	arg1	ultrasonication					1136:1150	long-term and strong ultrasonication	1115:1150	long-term and strong ultrasonication	1115:1150	However, long-term and strong ultrasonication will destroy the polymer structure to some extent.
33387758	7	11	dep	tensile	1038:1044	arg1	better					1031:1036	better	1031:1036	better	1031:1036	The composite film with ultrasonication exhibited better tensile and moisture barrier properties than the nonsonicated film.
33387758	1	12	theme	Ultrasonic	122:131	arg1	treatment					133:141	Ultrasonic treatment	122:141	Ultrasonic treatment	122:141	Ultrasonic treatment can improve the compatibility between a hydrophobic material and a hydrophilic polymer.
33387758	1	13	theme	hydrophobic	183:193	arg1	material					195:202	a hydrophobic material	181:202	a hydrophobic material	181:202	Ultrasonic treatment can improve the compatibility between a hydrophobic material and a hydrophilic polymer.
33387758	2	14	theme	surface	295:301	arg1	morphology					303:312	surface morphology	295:312	surface morphology	295:312	The light transmittance, crystalline structure, microstructure, surface morphology, moisture barrier, and mechanical properties of a composite film with or without ultrasonication were investigated.
33387758	6	15	theme	%	822:822	arg1	amplitude					824:832	70% amplitude	820:832	70% amplitude with a duration of 30 min	820:858	Compared to the control film, after ultrasonication at 70% amplitude with a duration of 30 min, the average roughness and maximum roughness declined from 212 nm to 17.6 nm and from 768.7 nm to 86.5 nm, respectively.
33387758	2	16	theme	mechanical	337:346	arg1	properties					348:357	mechanical properties	337:357	mechanical properties	337:357	The light transmittance, crystalline structure, microstructure, surface morphology, moisture barrier, and mechanical properties of a composite film with or without ultrasonication were investigated.
33387758	5	17	theme	film	667:670	arg1	surface					652:658	The surface	648:658	The surface of the film	648:670	The surface of the film became smooth and homogeneous after the film-form suspension underwent ultrasonic treatment.
33387758	6	18	theme	70	820:821	arg1	%					822:822	%	822:822	%	822:822	Compared to the control film, after ultrasonication at 70% amplitude with a duration of 30 min, the average roughness and maximum roughness declined from 212 nm to 17.6 nm and from 768.7 nm to 86.5 nm, respectively.
33387758	3	19	theme	light	462:466	arg1	transmittance					468:480	the film's light transmittance	451:480	the film's light transmittance	451:480	Ultrasound increases the film's light transmittance, resulting in a film that has good transparency.
33387758	1	20	theme	hydrophilic	210:220	arg1	polymer					222:228	a hydrophilic polymer	208:228	a hydrophilic polymer	208:228	Ultrasonic treatment can improve the compatibility between a hydrophobic material and a hydrophilic polymer.
33387758	0	21	theme	ultrasonication	11:25	arg1	Effects					0:6	Effects	0:6	Effects of ultrasonication on the properties of maize	0:52	Effects of ultrasonication on the properties of maize starch/stearic acid/ sodium carboxymethyl cellulose composite film.
33387758	4	22	theme	film	603:606	arg1	structure					578:586	the crystalline structure	562:586	the crystalline structure of the polymer film	562:606	Ultrasonication did not change the crystalline structure of the polymer film, but promoted V-type complex formation.
33387758	5	23	theme	ultrasonic	743:752	arg1	treatment					754:762	ultrasonic treatment	743:762	ultrasonic treatment	743:762	The surface of the film became smooth and homogeneous after the film-form suspension underwent ultrasonic treatment.
33387758	4	24	theme	polymer	595:601	arg1	film					603:606	the polymer film	591:606	the polymer film	591:606	Ultrasonication did not change the crystalline structure of the polymer film, but promoted V-type complex formation.
33387758	6	25	theme	maximum	887:893	arg1	roughness					895:903	maximum roughness	887:903	maximum roughness	887:903	Compared to the control film, after ultrasonication at 70% amplitude with a duration of 30 min, the average roughness and maximum roughness declined from 212 nm to 17.6 nm and from 768.7 nm to 86.5 nm, respectively.
33387758	2	26	theme	crystalline	256:266	arg1	structure					268:276	crystalline structure	256:276	crystalline structure	256:276	The light transmittance, crystalline structure, microstructure, surface morphology, moisture barrier, and mechanical properties of a composite film with or without ultrasonication were investigated.
33387758	6	27	from	amplitude	824:832	arg1	ultrasonication					801:815	ultrasonication	801:815	ultrasonication at 70% amplitude with a duration of 30 min	801:858	Compared to the control film, after ultrasonication at 70% amplitude with a duration of 30 min, the average roughness and maximum roughness declined from 212 nm to 17.6 nm and from 768.7 nm to 86.5 nm, respectively.
33387758	8	28	theme	polymer	1169:1175	arg1	structure					1177:1185	the polymer structure	1165:1185	the polymer structure	1165:1185	However, long-term and strong ultrasonication will destroy the polymer structure to some extent.
33387758	8	29	theme	strong	1129:1134	arg1	ultrasonication					1136:1150	long-term and strong ultrasonication	1115:1150	long-term and strong ultrasonication	1115:1150	However, long-term and strong ultrasonication will destroy the polymer structure to some extent.
33387758	7	30	theme	moisture	1050:1057	arg1	properties					1067:1076	better tensile and moisture barrier properties	1031:1076	properties	1067:1076	The composite film with ultrasonication exhibited better tensile and moisture barrier properties than the nonsonicated film.
33387758	7	31	with	film	995:998	arg1	ultrasonication					1005:1019	ultrasonication	1005:1019	ultrasonication	1005:1019	The composite film with ultrasonication exhibited better tensile and moisture barrier properties than the nonsonicated film.
33387758	0	32	theme	maize	48:52	arg1	properties					34:43	the properties	30:43	the properties of maize	30:52	Effects of ultrasonication on the properties of maize starch/stearic acid/ sodium carboxymethyl cellulose composite film.
33387758	2	33	theme	composite	364:372	arg1	film					374:377	a composite film	362:377	a composite film	362:377	The light transmittance, crystalline structure, microstructure, surface morphology, moisture barrier, and mechanical properties of a composite film with or without ultrasonication were investigated.
33387758	7	34	theme	barrier	1059:1065	arg1	properties					1067:1076	better tensile and moisture barrier properties	1031:1076	properties	1067:1076	The composite film with ultrasonication exhibited better tensile and moisture barrier properties than the nonsonicated film.
33387758	3	35	theme	good	512:515	arg1	transparency					517:528	good transparency	512:528	good transparency	512:528	Ultrasound increases the film's light transmittance, resulting in a film that has good transparency.
33387758	7	36	theme	nonsonicated	1087:1098	arg1	film					1100:1103	the nonsonicated film	1083:1103	the nonsonicated film	1083:1103	The composite film with ultrasonication exhibited better tensile and moisture barrier properties than the nonsonicated film.
33387758	6	37	theme	average	865:871	arg1	roughness					873:881	the average roughness	861:881	the average roughness	861:881	Compared to the control film, after ultrasonication at 70% amplitude with a duration of 30 min, the average roughness and maximum roughness declined from 212 nm to 17.6 nm and from 768.7 nm to 86.5 nm, respectively.
33387758	2	38	theme	light	235:239	arg1	transmittance					241:253	The light transmittance	231:253	The light transmittance	231:253	The light transmittance, crystalline structure, microstructure, surface morphology, moisture barrier, and mechanical properties of a composite film with or without ultrasonication were investigated.
33387758	4	39	theme	crystalline	566:576	arg1	structure					578:586	the crystalline structure	562:586	the crystalline structure of the polymer film	562:606	Ultrasonication did not change the crystalline structure of the polymer film, but promoted V-type complex formation.
33387758	5	40	theme	film-form	712:720	arg1	suspension					722:731	the film-form suspension	708:731	the film-form suspension underwent ultrasonic treatment	708:762	The surface of the film became smooth and homogeneous after the film-form suspension underwent ultrasonic treatment.
33387758	6	41	theme	control	781:787	arg1	film					789:792	the control film	777:792	the control film	777:792	Compared to the control film, after ultrasonication at 70% amplitude with a duration of 30 min, the average roughness and maximum roughness declined from 212 nm to 17.6 nm and from 768.7 nm to 86.5 nm, respectively.
33387758	4	42	theme	complex	629:635	arg1	formation					637:645	V-type complex formation	622:645	V-type complex formation	622:645	Ultrasonication did not change the crystalline structure of the polymer film, but promoted V-type complex formation.
33387758	0	43	theme	acid/	69:73	arg1	film					116:119	acid/ sodium carboxymethyl cellulose composite film	69:119	acid/ sodium carboxymethyl cellulose composite film	69:119	Effects of ultrasonication on the properties of maize starch/stearic acid/ sodium carboxymethyl cellulose composite film.
33387758	6	44	theme	30 min	853:858	arg1	duration					841:848	a duration	839:848	a duration of 30 min	839:858	Compared to the control film, after ultrasonication at 70% amplitude with a duration of 30 min, the average roughness and maximum roughness declined from 212 nm to 17.6 nm and from 768.7 nm to 86.5 nm, respectively.
33387758	6	45	with	amplitude	824:832	arg1	duration					841:848	a duration	839:848	a duration of 30 min	839:858	Compared to the control film, after ultrasonication at 70% amplitude with a duration of 30 min, the average roughness and maximum roughness declined from 212 nm to 17.6 nm and from 768.7 nm to 86.5 nm, respectively.
34655829	2	0	theme	lutein	499:504	arg1	encapsulation					506:518	lutein encapsulation	499:518	lutein encapsulation	499:518	To overcome these shortcomings, self-assembly composite nanoparticles from Stauntonia brachyanthera seed albumin (SBSA), gum Arabic (GA), and carboxymethylcellulose (CMC) were developed for lutein encapsulation.
34655829	1	1	theme	several	178:184	arg1	functions					197:205	several beneficial functions	178:205	several beneficial functions	178:205	Lutein is a carotenoid with several beneficial functions, but its poor water solubility, chemical instability, and low bioavailability limits its application.
34655829	1	2	theme	low	265:267	arg1	bioavailability					269:283	low bioavailability	265:283	low bioavailability	265:283	Lutein is a carotenoid with several beneficial functions, but its poor water solubility, chemical instability, and low bioavailability limits its application.
34655829	6	3	theme	hydrophobic	827:837	arg1	interactions					839:850	hydrophobic interactions	827:850	hydrophobic interactions	827:850	Hydrogen bonds, hydrophobic interactions, and electrostatic interactions were proved to derive the formation of nanoparticles.
34655829	1	4	theme	beneficial	186:195	arg1	functions					197:205	several beneficial functions	178:205	several beneficial functions	178:205	Lutein is a carotenoid with several beneficial functions, but its poor water solubility, chemical instability, and low bioavailability limits its application.
34655829	7	5	with	%	1028:1028	arg1	EE					1064:1065	EE	1064:1065	EE	1064:1065	The maximum effective loading capacity (LC) of the lutein in nanoparticles was 0.92 ± 0.01% with an encapsulation efficiency (EE) at 83.95 ± 0.98%.
34655829	7	5	with	%	1028:1028	arg1	efficiency					1052:1061	an encapsulation efficiency	1035:1061	an encapsulation efficiency (EE)	1035:1066	The maximum effective loading capacity (LC) of the lutein in nanoparticles was 0.92 ± 0.01% with an encapsulation efficiency (EE) at 83.95 ± 0.98%.
34655829	9	6	theme	17.50 ± 2.60	1260:1271	arg1	%					1272:1272	%	1272:1272	%	1272:1272	In addition, the bioaccessibility of lutein increased from 17.50 ± 2.60% to 46.80 ± 4.70% after encapsulation into nanoparticles.
34655829	0	7	from	Development	0:10	arg1	brachyanthera					102:114	carboxymethylcellulose-modified Stauntonia brachyanthera	59:114	carboxymethylcellulose-modified Stauntonia brachyanthera	59:114	Development of composite nanoparticles from gum Arabic and carboxymethylcellulose-modified Stauntonia brachyanthera seed albumin for lutein delivery.
34655829	0	7	from	Development	0:10	arg1	Arabic					48:53	gum Arabic	44:53	gum Arabic	44:53	Development of composite nanoparticles from gum Arabic and carboxymethylcellulose-modified Stauntonia brachyanthera seed albumin for lutein delivery.
34655829	1	8	with	carotenoid	162:171	arg1	functions					197:205	several beneficial functions	178:205	several beneficial functions	178:205	Lutein is a carotenoid with several beneficial functions, but its poor water solubility, chemical instability, and low bioavailability limits its application.
34655829	2	9	theme	seed	409:412	arg1	SBSA					423:426	SBSA	423:426	SBSA	423:426	To overcome these shortcomings, self-assembly composite nanoparticles from Stauntonia brachyanthera seed albumin (SBSA), gum Arabic (GA), and carboxymethylcellulose (CMC) were developed for lutein encapsulation.
34655829	2	9	theme	seed	409:412	arg1	albumin					414:420	seed albumin	409:420	Stauntonia brachyanthera seed albumin (SBSA)	384:427	To overcome these shortcomings, self-assembly composite nanoparticles from Stauntonia brachyanthera seed albumin (SBSA), gum Arabic (GA), and carboxymethylcellulose (CMC) were developed for lutein encapsulation.
34655829	7	10	from	%	1083:1083	arg1	capacity					968:975	The maximum effective loading capacity	938:975	The maximum effective loading capacity (LC) of the lutein in nanoparticles	938:1011	The maximum effective loading capacity (LC) of the lutein in nanoparticles was 0.92 ± 0.01% with an encapsulation efficiency (EE) at 83.95 ± 0.98%.
34655829	7	10	from	%	1083:1083	arg1	%					1028:1028	0.92 ± 0.01%	1017:1028	0.92 ± 0.01%	1017:1028	The maximum effective loading capacity (LC) of the lutein in nanoparticles was 0.92 ± 0.01% with an encapsulation efficiency (EE) at 83.95 ± 0.98%.
34655829	0	11	from	Arabic	48:53	arg1	Development					0:10	Development	0:10	Development of composite nanoparticles from gum Arabic and carboxymethylcellulose-modified Stauntonia brachyanthera	0:114	Development of composite nanoparticles from gum Arabic and carboxymethylcellulose-modified Stauntonia brachyanthera seed albumin for lutein delivery.
34655829	0	11	from	Arabic	48:53	arg1	nanoparticles					25:37	composite nanoparticles	15:37	composite nanoparticles from gum Arabic and carboxymethylcellulose-modified Stauntonia brachyanthera	15:114	Development of composite nanoparticles from gum Arabic and carboxymethylcellulose-modified Stauntonia brachyanthera seed albumin for lutein delivery.
34655829	6	12	theme	Hydrogen	811:818	arg1	bonds					820:824	Hydrogen bonds	811:824	Hydrogen bonds	811:824	Hydrogen bonds, hydrophobic interactions, and electrostatic interactions were proved to derive the formation of nanoparticles.
34655829	2	13	theme	gum	430:432	arg1	GA					442:443	GA	442:443	GA	442:443	To overcome these shortcomings, self-assembly composite nanoparticles from Stauntonia brachyanthera seed albumin (SBSA), gum Arabic (GA), and carboxymethylcellulose (CMC) were developed for lutein encapsulation.
34655829	2	13	theme	gum	430:432	arg1	Arabic					434:439	gum Arabic	430:439	gum Arabic (GA)	430:444	To overcome these shortcomings, self-assembly composite nanoparticles from Stauntonia brachyanthera seed albumin (SBSA), gum Arabic (GA), and carboxymethylcellulose (CMC) were developed for lutein encapsulation.
34655829	0	14	theme	lutein	133:138	arg1	delivery					140:147	lutein delivery	133:147	lutein delivery	133:147	Development of composite nanoparticles from gum Arabic and carboxymethylcellulose-modified Stauntonia brachyanthera seed albumin for lutein delivery.
34655829	2	15	theme	self-assembly	341:353	arg1	nanoparticles					365:377	self-assembly composite nanoparticles	341:377	self-assembly composite nanoparticles from Stauntonia brachyanthera seed albumin (SBSA), gum Arabic (GA), and carboxymethylcellulose (CMC)	341:478	To overcome these shortcomings, self-assembly composite nanoparticles from Stauntonia brachyanthera seed albumin (SBSA), gum Arabic (GA), and carboxymethylcellulose (CMC) were developed for lutein encapsulation.
34655829	7	16	theme	effective	950:958	arg1	capacity					968:975	The maximum effective loading capacity	938:975	The maximum effective loading capacity (LC) of the lutein in nanoparticles	938:1011	The maximum effective loading capacity (LC) of the lutein in nanoparticles was 0.92 ± 0.01% with an encapsulation efficiency (EE) at 83.95 ± 0.98%.
34655829	7	16	theme	effective	950:958	arg1	%					1028:1028	0.92 ± 0.01%	1017:1028	0.92 ± 0.01%	1017:1028	The maximum effective loading capacity (LC) of the lutein in nanoparticles was 0.92 ± 0.01% with an encapsulation efficiency (EE) at 83.95 ± 0.98%.
34655829	7	16	theme	effective	950:958	arg1	LC					978:979	LC	978:979	LC	978:979	The maximum effective loading capacity (LC) of the lutein in nanoparticles was 0.92 ± 0.01% with an encapsulation efficiency (EE) at 83.95 ± 0.98%.
34655829	9	17	theme	46.80 ± 4.70	1277:1288	arg1	%					1272:1272	%	1272:1272	%	1272:1272	In addition, the bioaccessibility of lutein increased from 17.50 ± 2.60% to 46.80 ± 4.70% after encapsulation into nanoparticles.
34655829	4	18	theme	self-assembly	689:701	arg1	method					703:708	a heat induced self-assembly method	674:708	a heat induced self-assembly method which were modified by GA and CMC	674:742	Followingly, the nanoparticles were prepared with SBSA through a heat induced self-assembly method which were modified by GA and CMC.
34655829	0	19	theme	nanoparticles	25:37	arg1	Development					0:10	Development	0:10	Development of composite nanoparticles from gum Arabic and carboxymethylcellulose-modified Stauntonia brachyanthera	0:114	Development of composite nanoparticles from gum Arabic and carboxymethylcellulose-modified Stauntonia brachyanthera seed albumin for lutein delivery.
34655829	9	20	theme	lutein	1238:1243	arg1	bioaccessibility					1218:1233	the bioaccessibility	1214:1233	the bioaccessibility of lutein	1214:1243	In addition, the bioaccessibility of lutein increased from 17.50 ± 2.60% to 46.80 ± 4.70% after encapsulation into nanoparticles.
34655829	8	21	theme	Heat	1086:1089	arg1	stability					1091:1099	Heat stability	1086:1099	Heat stability	1086:1099	Heat stability and storage stability of lutein were significantly enhanced after encapsulation into nanoparticles.
34655829	2	22	from	albumin	414:420	arg1	nanoparticles					365:377	self-assembly composite nanoparticles	341:377	self-assembly composite nanoparticles from Stauntonia brachyanthera seed albumin (SBSA), gum Arabic (GA), and carboxymethylcellulose (CMC)	341:478	To overcome these shortcomings, self-assembly composite nanoparticles from Stauntonia brachyanthera seed albumin (SBSA), gum Arabic (GA), and carboxymethylcellulose (CMC) were developed for lutein encapsulation.
34655829	2	23	dep	Stauntonia	384:393	arg1	brachyanthera					395:407	brachyanthera	395:407	brachyanthera	395:407	To overcome these shortcomings, self-assembly composite nanoparticles from Stauntonia brachyanthera seed albumin (SBSA), gum Arabic (GA), and carboxymethylcellulose (CMC) were developed for lutein encapsulation.
34655829	1	24	theme	poor	216:219	arg1	solubility					227:236	its poor water solubility	212:236	its poor water solubility	212:236	Lutein is a carotenoid with several beneficial functions, but its poor water solubility, chemical instability, and low bioavailability limits its application.
34655829	0	25	theme	composite	15:23	arg1	nanoparticles					25:37	composite nanoparticles	15:37	composite nanoparticles from gum Arabic and carboxymethylcellulose-modified Stauntonia brachyanthera	15:114	Development of composite nanoparticles from gum Arabic and carboxymethylcellulose-modified Stauntonia brachyanthera seed albumin for lutein delivery.
34655829	3	26	theme	physicochemical	568:582	arg1	properties					584:593	its physicochemical properties	564:593	its physicochemical properties	564:593	Firstly, SBSA was extracted from seeds and its physicochemical properties were evaluated.
34655829	1	27	theme	water	221:225	arg1	solubility					227:236	its poor water solubility	212:236	its poor water solubility	212:236	Lutein is a carotenoid with several beneficial functions, but its poor water solubility, chemical instability, and low bioavailability limits its application.
34655829	0	28	theme	gum	44:46	arg1	Arabic					48:53	gum Arabic	44:53	gum Arabic	44:53	Development of composite nanoparticles from gum Arabic and carboxymethylcellulose-modified Stauntonia brachyanthera seed albumin for lutein delivery.
34655829	0	29	from	brachyanthera	102:114	arg1	Development					0:10	Development	0:10	Development of composite nanoparticles from gum Arabic and carboxymethylcellulose-modified Stauntonia brachyanthera	0:114	Development of composite nanoparticles from gum Arabic and carboxymethylcellulose-modified Stauntonia brachyanthera seed albumin for lutein delivery.
34655829	0	29	from	brachyanthera	102:114	arg1	nanoparticles					25:37	composite nanoparticles	15:37	composite nanoparticles from gum Arabic and carboxymethylcellulose-modified Stauntonia brachyanthera	15:114	Development of composite nanoparticles from gum Arabic and carboxymethylcellulose-modified Stauntonia brachyanthera seed albumin for lutein delivery.
34655829	4	30	theme	induced	681:687	arg1	method					703:708	a heat induced self-assembly method	674:708	a heat induced self-assembly method which were modified by GA and CMC	674:742	Followingly, the nanoparticles were prepared with SBSA through a heat induced self-assembly method which were modified by GA and CMC.
34655829	5	31	theme	salt	795:798	arg1	stability					800:808	salt stability	795:808	salt stability	795:808	The nanoparticles exhibited good storage, pH, and salt stability.
34655829	5	32	theme	good	773:776	arg1	storage					778:784	good storage	773:784	good storage	773:784	The nanoparticles exhibited good storage, pH, and salt stability.
34655829	8	33	theme	lutein	1126:1131	arg1	stability					1091:1099	Heat stability	1086:1099	Heat stability	1086:1099	Heat stability and storage stability of lutein were significantly enhanced after encapsulation into nanoparticles.
34655829	8	33	theme	lutein	1126:1131	arg1	stability					1113:1121	storage stability	1105:1121	storage stability	1105:1121	Heat stability and storage stability of lutein were significantly enhanced after encapsulation into nanoparticles.
34655829	2	34	theme	Stauntonia	384:393	arg1	SBSA					423:426	SBSA	423:426	SBSA	423:426	To overcome these shortcomings, self-assembly composite nanoparticles from Stauntonia brachyanthera seed albumin (SBSA), gum Arabic (GA), and carboxymethylcellulose (CMC) were developed for lutein encapsulation.
34655829	2	34	theme	Stauntonia	384:393	arg1	albumin					414:420	seed albumin	409:420	Stauntonia brachyanthera seed albumin (SBSA)	384:427	To overcome these shortcomings, self-assembly composite nanoparticles from Stauntonia brachyanthera seed albumin (SBSA), gum Arabic (GA), and carboxymethylcellulose (CMC) were developed for lutein encapsulation.
34655829	2	35	from	carboxymethylcellulose	451:472	arg1	nanoparticles					365:377	self-assembly composite nanoparticles	341:377	self-assembly composite nanoparticles from Stauntonia brachyanthera seed albumin (SBSA), gum Arabic (GA), and carboxymethylcellulose (CMC)	341:478	To overcome these shortcomings, self-assembly composite nanoparticles from Stauntonia brachyanthera seed albumin (SBSA), gum Arabic (GA), and carboxymethylcellulose (CMC) were developed for lutein encapsulation.
34655829	8	36	theme	storage	1105:1111	arg1	stability					1113:1121	storage stability	1105:1121	storage stability	1105:1121	Heat stability and storage stability of lutein were significantly enhanced after encapsulation into nanoparticles.
34655829	6	37	theme	electrostatic	857:869	arg1	interactions					871:882	electrostatic interactions	857:882	electrostatic interactions	857:882	Hydrogen bonds, hydrophobic interactions, and electrostatic interactions were proved to derive the formation of nanoparticles.
34655829	4	38	mod	modified	721:728	arg3	GA					733:734	GA	733:734	GA	733:734	Followingly, the nanoparticles were prepared with SBSA through a heat induced self-assembly method which were modified by GA and CMC.
34655829	4	38	mod	modified	721:728	arg3	CMC					740:742	CMC	740:742	CMC	740:742	Followingly, the nanoparticles were prepared with SBSA through a heat induced self-assembly method which were modified by GA and CMC.
34655829	4	38	mod	modified	721:728	arg1	method					703:708	a heat induced self-assembly method	674:708	a heat induced self-assembly method which were modified by GA and CMC	674:742	Followingly, the nanoparticles were prepared with SBSA through a heat induced self-assembly method which were modified by GA and CMC.
34655829	7	39	theme	loading	960:966	arg1	capacity					968:975	The maximum effective loading capacity	938:975	The maximum effective loading capacity (LC) of the lutein in nanoparticles	938:1011	The maximum effective loading capacity (LC) of the lutein in nanoparticles was 0.92 ± 0.01% with an encapsulation efficiency (EE) at 83.95 ± 0.98%.
34655829	7	39	theme	loading	960:966	arg1	%					1028:1028	0.92 ± 0.01%	1017:1028	0.92 ± 0.01%	1017:1028	The maximum effective loading capacity (LC) of the lutein in nanoparticles was 0.92 ± 0.01% with an encapsulation efficiency (EE) at 83.95 ± 0.98%.
34655829	7	39	theme	loading	960:966	arg1	LC					978:979	LC	978:979	LC	978:979	The maximum effective loading capacity (LC) of the lutein in nanoparticles was 0.92 ± 0.01% with an encapsulation efficiency (EE) at 83.95 ± 0.98%.
34655829	7	40	theme	encapsulation	1038:1050	arg1	EE					1064:1065	EE	1064:1065	EE	1064:1065	The maximum effective loading capacity (LC) of the lutein in nanoparticles was 0.92 ± 0.01% with an encapsulation efficiency (EE) at 83.95 ± 0.98%.
34655829	7	40	theme	encapsulation	1038:1050	arg1	efficiency					1052:1061	an encapsulation efficiency	1035:1061	an encapsulation efficiency (EE)	1035:1066	The maximum effective loading capacity (LC) of the lutein in nanoparticles was 0.92 ± 0.01% with an encapsulation efficiency (EE) at 83.95 ± 0.98%.
34655829	7	41	from	capacity	968:975	arg1	nanoparticles					999:1011	nanoparticles	999:1011	nanoparticles	999:1011	The maximum effective loading capacity (LC) of the lutein in nanoparticles was 0.92 ± 0.01% with an encapsulation efficiency (EE) at 83.95 ± 0.98%.
34655829	7	42	theme	maximum	942:948	arg1	capacity					968:975	The maximum effective loading capacity	938:975	The maximum effective loading capacity (LC) of the lutein in nanoparticles	938:1011	The maximum effective loading capacity (LC) of the lutein in nanoparticles was 0.92 ± 0.01% with an encapsulation efficiency (EE) at 83.95 ± 0.98%.
34655829	7	42	theme	maximum	942:948	arg1	%					1028:1028	0.92 ± 0.01%	1017:1028	0.92 ± 0.01%	1017:1028	The maximum effective loading capacity (LC) of the lutein in nanoparticles was 0.92 ± 0.01% with an encapsulation efficiency (EE) at 83.95 ± 0.98%.
34655829	7	42	theme	maximum	942:948	arg1	LC					978:979	LC	978:979	LC	978:979	The maximum effective loading capacity (LC) of the lutein in nanoparticles was 0.92 ± 0.01% with an encapsulation efficiency (EE) at 83.95 ± 0.98%.
34655829	1	43	theme	chemical	239:246	arg1	instability					248:258	chemical instability	239:258	chemical instability	239:258	Lutein is a carotenoid with several beneficial functions, but its poor water solubility, chemical instability, and low bioavailability limits its application.
34655829	2	44	theme	composite	355:363	arg1	nanoparticles					365:377	self-assembly composite nanoparticles	341:377	self-assembly composite nanoparticles from Stauntonia brachyanthera seed albumin (SBSA), gum Arabic (GA), and carboxymethylcellulose (CMC)	341:478	To overcome these shortcomings, self-assembly composite nanoparticles from Stauntonia brachyanthera seed albumin (SBSA), gum Arabic (GA), and carboxymethylcellulose (CMC) were developed for lutein encapsulation.
34655829	0	45	theme	Stauntonia	91:100	arg1	brachyanthera					102:114	carboxymethylcellulose-modified Stauntonia brachyanthera	59:114	carboxymethylcellulose-modified Stauntonia brachyanthera	59:114	Development of composite nanoparticles from gum Arabic and carboxymethylcellulose-modified Stauntonia brachyanthera seed albumin for lutein delivery.
34655829	2	46	from	Arabic	434:439	arg1	nanoparticles					365:377	self-assembly composite nanoparticles	341:377	self-assembly composite nanoparticles from Stauntonia brachyanthera seed albumin (SBSA), gum Arabic (GA), and carboxymethylcellulose (CMC)	341:478	To overcome these shortcomings, self-assembly composite nanoparticles from Stauntonia brachyanthera seed albumin (SBSA), gum Arabic (GA), and carboxymethylcellulose (CMC) were developed for lutein encapsulation.
34655829	6	47	theme	nanoparticles	923:935	arg1	formation					910:918	the formation	906:918	the formation of nanoparticles	906:935	Hydrogen bonds, hydrophobic interactions, and electrostatic interactions were proved to derive the formation of nanoparticles.
34655829	0	48	theme	carboxymethylcellulose-modified	59:89	arg1	brachyanthera					102:114	carboxymethylcellulose-modified Stauntonia brachyanthera	59:114	carboxymethylcellulose-modified Stauntonia brachyanthera	59:114	Development of composite nanoparticles from gum Arabic and carboxymethylcellulose-modified Stauntonia brachyanthera seed albumin for lutein delivery.
34655829	7	49	theme	lutein	989:994	arg1	capacity					968:975	The maximum effective loading capacity	938:975	The maximum effective loading capacity (LC) of the lutein in nanoparticles	938:1011	The maximum effective loading capacity (LC) of the lutein in nanoparticles was 0.92 ± 0.01% with an encapsulation efficiency (EE) at 83.95 ± 0.98%.
34655829	7	49	theme	lutein	989:994	arg1	%					1028:1028	0.92 ± 0.01%	1017:1028	0.92 ± 0.01%	1017:1028	The maximum effective loading capacity (LC) of the lutein in nanoparticles was 0.92 ± 0.01% with an encapsulation efficiency (EE) at 83.95 ± 0.98%.
34655829	7	49	theme	lutein	989:994	arg1	LC					978:979	LC	978:979	LC	978:979	The maximum effective loading capacity (LC) of the lutein in nanoparticles was 0.92 ± 0.01% with an encapsulation efficiency (EE) at 83.95 ± 0.98%.
34655829	9	50	dep	%	1272:1272	arg1	to					1274:1275	to	1274:1275	to	1274:1275	In addition, the bioaccessibility of lutein increased from 17.50 ± 2.60% to 46.80 ± 4.70% after encapsulation into nanoparticles.
33990299	6	0	from	supernatant	692:702	arg1	proteins					665:672	The secreted proteins	652:672	The secreted proteins from each culture supernatant	652:702	The secreted proteins from each culture supernatant were tested for their polysaccharide-degrading ability, and the composition of secreted CAZymes in each sample was determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
33990299	17	1	theme	proteomic	2305:2313	arg1	approaches					2328:2337	biochemical, proteomic, and genomic approaches	2292:2337	biochemical, proteomic, and genomic approaches	2292:2337	By combining biochemical, proteomic, and genomic approaches, we discovered a novel mannose and mannobiose responsive transcriptional regulator, SsManR, which selectively regulates three α-1,2-mannosidase-coding genes.
33990299	2	2	theme	insect	143:148	arg1	sp					182:183	The cellulolytic insect symbiont bacterium Streptomyces sp	126:183	The cellulolytic insect symbiont bacterium Streptomyces sp.	126:184	The cellulolytic insect symbiont bacterium Streptomyces sp.
33990299	5	3	theme	sole	609:612	arg1	sources					621:627	sole carbon sources	609:627	sole carbon sources in the culture medium	609:649	SirexAA-E grew well on mannose, carboxymethyl cellulose (CMC), and locust bean gum (LBG) as sole carbon sources in the culture medium.
33990299	6	4	theme	secreted	783:790	arg1	CAZymes					792:798	secreted CAZymes	783:798	secreted CAZymes	783:798	The secreted proteins from each culture supernatant were tested for their polysaccharide-degrading ability, and the composition of secreted CAZymes in each sample was determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
33990299	19	5	dep	suggest	2655:2661	arg1	sp					2685:2686	the Streptomyces sp	2668:2686	the Streptomyces sp	2668:2686	Overall, our findings suggest that the Streptomyces sp.
33990299	20	6	theme	transcriptional	2767:2781	arg1	repressors					2783:2792	two different transcriptional repressors	2753:2792	two different transcriptional repressors that regulate the secretion of the plant cell wall-degrading enzymes to extract carbon sources in the host environment	2753:2911	SirexAA-E responds to mannose and mannooligosaccharides through two different transcriptional repressors that regulate the secretion of the plant cell wall-degrading enzymes to extract carbon sources in the host environment.
33990299	9	7	theme	sequence	1163:1170	arg1	motif					1172:1176	a unique 12-bp palindromic sequence motif	1136:1176	a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes	1136:1296	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	3	8	from	polysaccharides	311:325	arg1	wall					345:348	the plant cell wall	330:348	the plant cell wall	330:348	strain SirexAA-E secretes a suite of carbohydrate-active enzymes (CAZymes), which are involved in the degradation of various polysaccharides in the plant cell wall, in response to the available carbon sources.
33990299	17	9	theme	responsive	2385:2394	arg1	SsManR					2423:2428	SsManR	2423:2428	SsManR	2423:2428	By combining biochemical, proteomic, and genomic approaches, we discovered a novel mannose and mannobiose responsive transcriptional regulator, SsManR, which selectively regulates three α-1,2-mannosidase-coding genes.
33990299	17	9	theme	responsive	2385:2394	arg1	regulator					2412:2420	a novel mannose and mannobiose responsive transcriptional regulator	2354:2420	a novel mannose and mannobiose responsive transcriptional regulator	2354:2420	By combining biochemical, proteomic, and genomic approaches, we discovered a novel mannose and mannobiose responsive transcriptional regulator, SsManR, which selectively regulates three α-1,2-mannosidase-coding genes.
33990299	18	10	theme	described	2538:2546	arg1	SsCebR					2581:2586	SsCebR	2581:2586	SsCebR	2581:2586	We also demonstrated that the previously described cellobiose responsive regulator, SsCebR, could use mannobiose as an effector ligand.
33990299	18	10	theme	described	2538:2546	arg1	regulator					2570:2578	the previously described cellobiose responsive regulator	2523:2578	the previously described cellobiose responsive regulator	2523:2578	We also demonstrated that the previously described cellobiose responsive regulator, SsCebR, could use mannobiose as an effector ligand.
33990299	6	11	theme	chromatography-tandem	840:860	arg1	spectrometry					867:878	liquid chromatography-tandem mass spectrometry	833:878	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	833:889	The secreted proteins from each culture supernatant were tested for their polysaccharide-degrading ability, and the composition of secreted CAZymes in each sample was determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
33990299	6	11	theme	chromatography-tandem	840:860	arg1	LC-MS/MS					881:888	LC-MS/MS	881:888	LC-MS/MS	881:888	The secreted proteins from each culture supernatant were tested for their polysaccharide-degrading ability, and the composition of secreted CAZymes in each sample was determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
33990299	20	12	theme	enzymes	2855:2861	arg1	secretion					2812:2820	the secretion	2808:2820	the secretion of the plant cell wall-degrading enzymes to extract carbon sources in the host environment	2808:2911	SirexAA-E responds to mannose and mannooligosaccharides through two different transcriptional repressors that regulate the secretion of the plant cell wall-degrading enzymes to extract carbon sources in the host environment.
33990299	9	13	theme	locations	1183:1191	arg1	locations					1183:1191	4 locations	1181:1191	4 locations	1181:1191	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	9	13	theme	locations	1183:1191	arg1	two					1218:1220	two	1218:1220	two	1218:1220	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	20	14	from	sources	2881:2887	arg1	environment					2901:2911	the host environment	2892:2911	the host environment	2892:2911	SirexAA-E responds to mannose and mannooligosaccharides through two different transcriptional repressors that regulate the secretion of the plant cell wall-degrading enzymes to extract carbon sources in the host environment.
33990299	3	15	theme	various	303:309	arg1	polysaccharides					311:325	various polysaccharides	303:325	various polysaccharides in the plant cell wall	303:348	strain SirexAA-E secretes a suite of carbohydrate-active enzymes (CAZymes), which are involved in the degradation of various polysaccharides in the plant cell wall, in response to the available carbon sources.
33990299	20	16	theme	extract	2866:2872	arg1	sources					2881:2887	extract carbon sources	2866:2887	extract carbon sources in the host environment	2866:2911	SirexAA-E responds to mannose and mannooligosaccharides through two different transcriptional repressors that regulate the secretion of the plant cell wall-degrading enzymes to extract carbon sources in the host environment.
33990299	9	17	theme	12-bp	1145:1149	arg1	motif					1172:1176	a unique 12-bp palindromic sequence motif	1136:1176	a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes	1136:1296	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	12	18	theme	SsCebR	1899:1904	arg1	regulation					1869:1878	the regulation	1865:1878	the regulation of both SsManR and SsCebR	1865:1904	As hypothesized, the composition of the mannobiose secretome combined sets of CAZymes found in both LBG and CMC secretomes, and thus they are likely under the regulation of both SsManR and SsCebR.
33990299	4	19	theme	bacterium	451:459	arg1	response					434:441	a poorly understood response	414:441	a poorly understood response of this bacterium to mannan, one of the major plant cell wall components	414:514	Here, we examined a poorly understood response of this bacterium to mannan, one of the major plant cell wall components.
33990299	18	20	theme	responsive	2559:2568	arg1	SsCebR					2581:2586	SsCebR	2581:2586	SsCebR	2581:2586	We also demonstrated that the previously described cellobiose responsive regulator, SsCebR, could use mannobiose as an effector ligand.
33990299	18	20	theme	responsive	2559:2568	arg1	regulator					2570:2578	the previously described cellobiose responsive regulator	2523:2578	the previously described cellobiose responsive regulator	2523:2578	We also demonstrated that the previously described cellobiose responsive regulator, SsCebR, could use mannobiose as an effector ligand.
33990299	20	21	theme	plant	2829:2833	arg1	enzymes					2855:2861	the plant cell wall-degrading enzymes	2825:2861	the plant cell wall-degrading enzymes	2825:2861	SirexAA-E responds to mannose and mannooligosaccharides through two different transcriptional repressors that regulate the secretion of the plant cell wall-degrading enzymes to extract carbon sources in the host environment.
33990299	3	22	theme	plant	334:338	arg1	wall					345:348	the plant cell wall	330:348	the plant cell wall	330:348	strain SirexAA-E secretes a suite of carbohydrate-active enzymes (CAZymes), which are involved in the degradation of various polysaccharides in the plant cell wall, in response to the available carbon sources.
33990299	5	23	theme	culture	636:642	arg1	medium					644:649	the culture medium	632:649	the culture medium	632:649	SirexAA-E grew well on mannose, carboxymethyl cellulose (CMC), and locust bean gum (LBG) as sole carbon sources in the culture medium.
33990299	9	24	from	locations	1183:1191	arg1	genome					1210:1215	the SirexAA-E genome	1196:1215	the SirexAA-E genome	1196:1215	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	9	24	from	locations	1183:1191	arg1	motif					1172:1176	a unique 12-bp palindromic sequence motif	1136:1176	a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes	1136:1296	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	11	25	theme	regulators	1619:1628	arg1	control					1600:1606	the control	1596:1606	the control of the two regulators	1596:1628	To test whether mannobiose induces the sets of genes under the control of the two regulators, SirexAA-E was grown on mannobiose, and the secretome composition was analyzed.
33990299	3	26	from	wall	345:348	arg1	degradation					288:298	the degradation	284:298	the degradation of various polysaccharides in the plant cell wall	284:348	strain SirexAA-E secretes a suite of carbohydrate-active enzymes (CAZymes), which are involved in the degradation of various polysaccharides in the plant cell wall, in response to the available carbon sources.
33990299	4	27	theme	understood	423:432	arg1	response					434:441	a poorly understood response	414:441	a poorly understood response of this bacterium to mannan, one of the major plant cell wall components	414:514	Here, we examined a poorly understood response of this bacterium to mannan, one of the major plant cell wall components.
33990299	7	28	theme	mannan	966:971	arg1	enzymes					997:1003	mannan and cellulose-degrading enzymes	966:1003	mannan and cellulose-degrading enzymes	966:1003	The results indicated that mannose, LBG, and CMC induced the secretion of mannan and cellulose-degrading enzymes.
33990299	13	29	dep	sp	1930:1931	arg1	IMPORTANCEStreptomyces					1907:1928	IMPORTANCEStreptomyces	1907:1928	IMPORTANCEStreptomyces	1907:1928	IMPORTANCEStreptomyces sp.
33990299	8	30	from	growth	1076:1081	arg1	LBG					1098:1100	LBG	1098:1100	LBG	1098:1100	Interestingly, two α-1,2-mannosidases were abundantly secreted during growth on mannose and LBG.
33990299	8	30	from	growth	1076:1081	arg1	mannose					1086:1092	mannose	1086:1092	mannose	1086:1092	Interestingly, two α-1,2-mannosidases were abundantly secreted during growth on mannose and LBG.
33990299	3	31	theme	strain	186:191	arg1	SirexAA-E					193:201	strain SirexAA-E	186:201	strain SirexAA-E	186:201	strain SirexAA-E secretes a suite of carbohydrate-active enzymes (CAZymes), which are involved in the degradation of various polysaccharides in the plant cell wall, in response to the available carbon sources.
33990299	0	32	theme	Bacterium	81:89	arg1	sp					104:105	the Insect-Associated Cellulolytic Bacterium Streptomyces sp	46:105	the Insect-Associated Cellulolytic Bacterium Streptomyces sp	46:105	Mannose- and Mannobiose-Specific Responses of the Insect-Associated Cellulolytic Bacterium Streptomyces sp.
33990299	4	33	theme	plant	489:493	arg1	components					505:514	the major plant cell wall components	479:514	the major plant cell wall components	479:514	Here, we examined a poorly understood response of this bacterium to mannan, one of the major plant cell wall components.
33990299	3	34	dep	sources	387:393	arg1	response					354:361	response	354:361	response	354:361	strain SirexAA-E secretes a suite of carbohydrate-active enzymes (CAZymes), which are involved in the degradation of various polysaccharides in the plant cell wall, in response to the available carbon sources.
33990299	0	35	theme	sp	104:105	arg1	Responses					33:41	Mannose- and Mannobiose-Specific Responses	0:41	Mannose- and Mannobiose-Specific Responses of the Insect-Associated Cellulolytic Bacterium Streptomyces sp	0:105	Mannose- and Mannobiose-Specific Responses of the Insect-Associated Cellulolytic Bacterium Streptomyces sp.
33990299	3	36	theme	available	370:378	arg1	sources					387:393	the available carbon sources	366:393	the available carbon sources	366:393	strain SirexAA-E secretes a suite of carbohydrate-active enzymes (CAZymes), which are involved in the degradation of various polysaccharides in the plant cell wall, in response to the available carbon sources.
33990299	4	37	theme	wall	500:503	arg1	components					505:514	the major plant cell wall components	479:514	the major plant cell wall components	479:514	Here, we examined a poorly understood response of this bacterium to mannan, one of the major plant cell wall components.
33990299	10	38	theme	reported	1426:1433	arg1	SsCebR					1468:1473	SsCebR	1468:1473	SsCebR	1468:1473	Furthermore, the previously reported cellobiose-responsive repressor, SsCebR, was determined to also use mannobiose as an effector ligand.
33990299	10	38	theme	reported	1426:1433	arg1	repressor					1457:1465	the previously reported cellobiose-responsive repressor	1411:1465	the previously reported cellobiose-responsive repressor	1411:1465	Furthermore, the previously reported cellobiose-responsive repressor, SsCebR, was determined to also use mannobiose as an effector ligand.
33990299	9	39	theme	above-mentioned	1258:1272	arg1	genes					1292:1296	the above-mentioned α-1,2-mannosidase genes	1254:1296	the above-mentioned α-1,2-mannosidase genes	1254:1296	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	5	40	theme	locust	584:589	arg1	LBG					601:603	LBG	601:603	LBG	601:603	SirexAA-E grew well on mannose, carboxymethyl cellulose (CMC), and locust bean gum (LBG) as sole carbon sources in the culture medium.
33990299	5	40	theme	locust	584:589	arg1	gum					596:598	locust bean gum	584:598	locust bean gum (LBG)	584:604	SirexAA-E grew well on mannose, carboxymethyl cellulose (CMC), and locust bean gum (LBG) as sole carbon sources in the culture medium.
33990299	6	41	theme	secreted	656:663	arg1	proteins					665:672	The secreted proteins	652:672	The secreted proteins from each culture supernatant	652:702	The secreted proteins from each culture supernatant were tested for their polysaccharide-degrading ability, and the composition of secreted CAZymes in each sample was determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
33990299	14	42	theme	biomass-harvesting	1969:1986	arg1	insects					1988:1994	biomass-harvesting insects	1969:1994	biomass-harvesting insects	1969:1994	SirexAA-E, a microbial symbiont of biomass-harvesting insects, secretes a suite of polysaccharide-degrading enzymes dependent on the available carbon sources.
33990299	14	43	theme	carbon	2077:2082	arg1	sources					2084:2090	the available carbon sources	2063:2090	the available carbon sources	2063:2090	SirexAA-E, a microbial symbiont of biomass-harvesting insects, secretes a suite of polysaccharide-degrading enzymes dependent on the available carbon sources.
33990299	18	44	theme	effector	2616:2623	arg1	mannobiose					2599:2608	mannobiose	2599:2608	mannobiose	2599:2608	We also demonstrated that the previously described cellobiose responsive regulator, SsCebR, could use mannobiose as an effector ligand.
33990299	18	44	theme	effector	2616:2623	arg1	ligand					2625:2630	an effector ligand	2613:2630	an effector ligand	2613:2630	We also demonstrated that the previously described cellobiose responsive regulator, SsCebR, could use mannobiose as an effector ligand.
33990299	0	45	theme	Mannose-	0:7	arg1	Responses					33:41	Mannose- and Mannobiose-Specific Responses	0:41	Mannose- and Mannobiose-Specific Responses of the Insect-Associated Cellulolytic Bacterium Streptomyces sp	0:105	Mannose- and Mannobiose-Specific Responses of the Insect-Associated Cellulolytic Bacterium Streptomyces sp.
33990299	12	46	located	found	1796:1800	arg1	secretomes					1822:1831	both LBG and CMC secretomes	1805:1831	secretomes	1822:1831	As hypothesized, the composition of the mannobiose secretome combined sets of CAZymes found in both LBG and CMC secretomes, and thus they are likely under the regulation of both SsManR and SsCebR.
33990299	12	46	located	found	1796:1800	arg2	CAZymes					1788:1794	CAZymes	1788:1794	CAZymes found in both LBG and CMC secretomes	1788:1831	As hypothesized, the composition of the mannobiose secretome combined sets of CAZymes found in both LBG and CMC secretomes, and thus they are likely under the regulation of both SsManR and SsCebR.
33990299	3	47	theme	carbohydrate-active	223:241	arg1	CAZymes					252:258	CAZymes	252:258	CAZymes	252:258	strain SirexAA-E secretes a suite of carbohydrate-active enzymes (CAZymes), which are involved in the degradation of various polysaccharides in the plant cell wall, in response to the available carbon sources.
33990299	3	47	theme	carbohydrate-active	223:241	arg1	enzymes					243:249	carbohydrate-active enzymes	223:249	carbohydrate-active enzymes (CAZymes)	223:259	strain SirexAA-E secretes a suite of carbohydrate-active enzymes (CAZymes), which are involved in the degradation of various polysaccharides in the plant cell wall, in response to the available carbon sources.
33990299	16	48	theme	bacterium	2220:2228	arg1	response					2203:2210	the response	2199:2210	the response of this bacterium to mannose, mannobiose, and galactomannan (LBG)	2199:2276	In this study, we investigated the response of this bacterium to mannose, mannobiose, and galactomannan (LBG).
33990299	0	49	theme	Mannobiose-Specific	13:31	arg1	Responses					33:41	Mannose- and Mannobiose-Specific Responses	0:41	Mannose- and Mannobiose-Specific Responses of the Insect-Associated Cellulolytic Bacterium Streptomyces sp	0:105	Mannose- and Mannobiose-Specific Responses of the Insect-Associated Cellulolytic Bacterium Streptomyces sp.
33990299	6	50	theme	liquid	833:838	arg1	spectrometry					867:878	liquid chromatography-tandem mass spectrometry	833:878	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	833:889	The secreted proteins from each culture supernatant were tested for their polysaccharide-degrading ability, and the composition of secreted CAZymes in each sample was determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
33990299	6	50	theme	liquid	833:838	arg1	LC-MS/MS					881:888	LC-MS/MS	881:888	LC-MS/MS	881:888	The secreted proteins from each culture supernatant were tested for their polysaccharide-degrading ability, and the composition of secreted CAZymes in each sample was determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
33990299	14	51	theme	dependent	2050:2058	arg1	enzymes					2042:2048	polysaccharide-degrading enzymes	2017:2048	polysaccharide-degrading enzymes dependent on the available carbon sources	2017:2090	SirexAA-E, a microbial symbiont of biomass-harvesting insects, secretes a suite of polysaccharide-degrading enzymes dependent on the available carbon sources.
33990299	11	52	theme	secretome	1674:1682	arg1	composition					1684:1694	the secretome composition	1670:1694	the secretome composition	1670:1694	To test whether mannobiose induces the sets of genes under the control of the two regulators, SirexAA-E was grown on mannobiose, and the secretome composition was analyzed.
33990299	9	53	theme	identified	1318:1327	arg1	SsManR					1390:1395	SsManR	1390:1395	SsManR	1390:1395	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	9	53	theme	identified	1318:1327	arg1	regulator					1379:1387	a newly identified mannose and mannobiose-responsive transcriptional regulator	1310:1387	a newly identified mannose and mannobiose-responsive transcriptional regulator	1310:1387	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	17	54	theme	novel	2356:2360	arg1	SsManR					2423:2428	SsManR	2423:2428	SsManR	2423:2428	By combining biochemical, proteomic, and genomic approaches, we discovered a novel mannose and mannobiose responsive transcriptional regulator, SsManR, which selectively regulates three α-1,2-mannosidase-coding genes.
33990299	17	54	theme	novel	2356:2360	arg1	regulator					2412:2420	a novel mannose and mannobiose responsive transcriptional regulator	2354:2420	a novel mannose and mannobiose responsive transcriptional regulator	2354:2420	By combining biochemical, proteomic, and genomic approaches, we discovered a novel mannose and mannobiose responsive transcriptional regulator, SsManR, which selectively regulates three α-1,2-mannosidase-coding genes.
33990299	10	55	theme	effector	1520:1527	arg1	ligand					1529:1534	an effector ligand	1517:1534	an effector ligand	1517:1534	Furthermore, the previously reported cellobiose-responsive repressor, SsCebR, was determined to also use mannobiose as an effector ligand.
33990299	10	55	theme	effector	1520:1527	arg1	mannobiose					1503:1512	mannobiose	1503:1512	mannobiose	1503:1512	Furthermore, the previously reported cellobiose-responsive repressor, SsCebR, was determined to also use mannobiose as an effector ligand.
33990299	9	56	theme	genomic	1109:1115	arg1	analysis					1117:1124	genomic analysis	1109:1124	genomic analysis	1109:1124	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	0	57	theme	Insect-Associated	50:66	arg1	sp					104:105	the Insect-Associated Cellulolytic Bacterium Streptomyces sp	46:105	the Insect-Associated Cellulolytic Bacterium Streptomyces sp	46:105	Mannose- and Mannobiose-Specific Responses of the Insect-Associated Cellulolytic Bacterium Streptomyces sp.
33990299	12	58	theme	LBG	1810:1812	arg1	secretomes					1822:1831	both LBG and CMC secretomes	1805:1831	secretomes	1822:1831	As hypothesized, the composition of the mannobiose secretome combined sets of CAZymes found in both LBG and CMC secretomes, and thus they are likely under the regulation of both SsManR and SsCebR.
33990299	9	59	theme	transcriptional	1363:1377	arg1	SsManR					1390:1395	SsManR	1390:1395	SsManR	1390:1395	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	9	59	theme	transcriptional	1363:1377	arg1	regulator					1379:1387	a newly identified mannose and mannobiose-responsive transcriptional regulator	1310:1387	a newly identified mannose and mannobiose-responsive transcriptional regulator	1310:1387	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	12	60	theme	CMC	1818:1820	arg1	secretomes					1822:1831	both LBG and CMC secretomes	1805:1831	secretomes	1822:1831	As hypothesized, the composition of the mannobiose secretome combined sets of CAZymes found in both LBG and CMC secretomes, and thus they are likely under the regulation of both SsManR and SsCebR.
33990299	12	61	theme	combined	1771:1778	arg1	sets					1780:1783	combined sets	1771:1783	combined sets of CAZymes found in both LBG and CMC secretomes	1771:1831	As hypothesized, the composition of the mannobiose secretome combined sets of CAZymes found in both LBG and CMC secretomes, and thus they are likely under the regulation of both SsManR and SsCebR.
33990299	12	61	theme	combined	1771:1778	arg1	CAZymes					1788:1794	CAZymes	1788:1794	CAZymes found in both LBG and CMC secretomes	1788:1831	As hypothesized, the composition of the mannobiose secretome combined sets of CAZymes found in both LBG and CMC secretomes, and thus they are likely under the regulation of both SsManR and SsCebR.
33990299	6	62	theme	culture	684:690	arg1	supernatant					692:702	each culture supernatant	679:702	each culture supernatant	679:702	The secreted proteins from each culture supernatant were tested for their polysaccharide-degrading ability, and the composition of secreted CAZymes in each sample was determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
33990299	9	63	theme	mannose	1329:1335	arg1	SsManR					1390:1395	SsManR	1390:1395	SsManR	1390:1395	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	9	63	theme	mannose	1329:1335	arg1	regulator					1379:1387	a newly identified mannose and mannobiose-responsive transcriptional regulator	1310:1387	a newly identified mannose and mannobiose-responsive transcriptional regulator	1310:1387	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	2	64	theme	symbiont	150:157	arg1	sp					182:183	The cellulolytic insect symbiont bacterium Streptomyces sp	126:183	The cellulolytic insect symbiont bacterium Streptomyces sp.	126:184	The cellulolytic insect symbiont bacterium Streptomyces sp.
33990299	20	65	theme	different	2757:2765	arg1	repressors					2783:2792	two different transcriptional repressors	2753:2792	two different transcriptional repressors that regulate the secretion of the plant cell wall-degrading enzymes to extract carbon sources in the host environment	2753:2911	SirexAA-E responds to mannose and mannooligosaccharides through two different transcriptional repressors that regulate the secretion of the plant cell wall-degrading enzymes to extract carbon sources in the host environment.
33990299	9	66	theme	unique	1138:1143	arg1	motif					1172:1176	a unique 12-bp palindromic sequence motif	1136:1176	a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes	1136:1296	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	14	67	theme	polysaccharide-degrading	2017:2040	arg1	enzymes					2042:2048	polysaccharide-degrading enzymes	2017:2048	polysaccharide-degrading enzymes dependent on the available carbon sources	2017:2090	SirexAA-E, a microbial symbiont of biomass-harvesting insects, secretes a suite of polysaccharide-degrading enzymes dependent on the available carbon sources.
33990299	3	68	from	degradation	288:298	arg1	wall					345:348	the plant cell wall	330:348	the plant cell wall	330:348	strain SirexAA-E secretes a suite of carbohydrate-active enzymes (CAZymes), which are involved in the degradation of various polysaccharides in the plant cell wall, in response to the available carbon sources.
33990299	2	69	theme	cellulolytic	130:141	arg1	sp					182:183	The cellulolytic insect symbiont bacterium Streptomyces sp	126:183	The cellulolytic insect symbiont bacterium Streptomyces sp.	126:184	The cellulolytic insect symbiont bacterium Streptomyces sp.
33990299	5	70	theme	carbon	614:619	arg1	sources					621:627	sole carbon sources	609:627	sole carbon sources in the culture medium	609:649	SirexAA-E grew well on mannose, carboxymethyl cellulose (CMC), and locust bean gum (LBG) as sole carbon sources in the culture medium.
33990299	9	71	theme	palindromic	1151:1161	arg1	motif					1172:1176	a unique 12-bp palindromic sequence motif	1136:1176	a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes	1136:1296	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	12	72	theme	mannobiose	1750:1759	arg1	composition					1731:1741	the composition	1727:1741	the composition of the mannobiose	1727:1759	As hypothesized, the composition of the mannobiose secretome combined sets of CAZymes found in both LBG and CMC secretomes, and thus they are likely under the regulation of both SsManR and SsCebR.
33990299	20	73	theme	carbon	2874:2879	arg1	sources					2881:2887	extract carbon sources	2866:2887	extract carbon sources in the host environment	2866:2911	SirexAA-E responds to mannose and mannooligosaccharides through two different transcriptional repressors that regulate the secretion of the plant cell wall-degrading enzymes to extract carbon sources in the host environment.
33990299	17	74	theme	mannobiose	2374:2383	arg1	SsManR					2423:2428	SsManR	2423:2428	SsManR	2423:2428	By combining biochemical, proteomic, and genomic approaches, we discovered a novel mannose and mannobiose responsive transcriptional regulator, SsManR, which selectively regulates three α-1,2-mannosidase-coding genes.
33990299	17	74	theme	mannobiose	2374:2383	arg1	regulator					2412:2420	a novel mannose and mannobiose responsive transcriptional regulator	2354:2420	a novel mannose and mannobiose responsive transcriptional regulator	2354:2420	By combining biochemical, proteomic, and genomic approaches, we discovered a novel mannose and mannobiose responsive transcriptional regulator, SsManR, which selectively regulates three α-1,2-mannosidase-coding genes.
33990299	17	75	theme	genomic	2320:2326	arg1	approaches					2328:2337	biochemical, proteomic, and genomic approaches	2292:2337	biochemical, proteomic, and genomic approaches	2292:2337	By combining biochemical, proteomic, and genomic approaches, we discovered a novel mannose and mannobiose responsive transcriptional regulator, SsManR, which selectively regulates three α-1,2-mannosidase-coding genes.
33990299	6	76	theme	mass	862:865	arg1	spectrometry					867:878	liquid chromatography-tandem mass spectrometry	833:878	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	833:889	The secreted proteins from each culture supernatant were tested for their polysaccharide-degrading ability, and the composition of secreted CAZymes in each sample was determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
33990299	6	76	theme	mass	862:865	arg1	LC-MS/MS					881:888	LC-MS/MS	881:888	LC-MS/MS	881:888	The secreted proteins from each culture supernatant were tested for their polysaccharide-degrading ability, and the composition of secreted CAZymes in each sample was determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
33990299	17	77	theme	transcriptional	2396:2410	arg1	SsManR					2423:2428	SsManR	2423:2428	SsManR	2423:2428	By combining biochemical, proteomic, and genomic approaches, we discovered a novel mannose and mannobiose responsive transcriptional regulator, SsManR, which selectively regulates three α-1,2-mannosidase-coding genes.
33990299	17	77	theme	transcriptional	2396:2410	arg1	regulator					2412:2420	a novel mannose and mannobiose responsive transcriptional regulator	2354:2420	a novel mannose and mannobiose responsive transcriptional regulator	2354:2420	By combining biochemical, proteomic, and genomic approaches, we discovered a novel mannose and mannobiose responsive transcriptional regulator, SsManR, which selectively regulates three α-1,2-mannosidase-coding genes.
33990299	2	78	theme	Streptomyces	169:180	arg1	sp					182:183	The cellulolytic insect symbiont bacterium Streptomyces sp	126:183	The cellulolytic insect symbiont bacterium Streptomyces sp.	126:184	The cellulolytic insect symbiont bacterium Streptomyces sp.
33990299	9	79	dep	along	1299:1303	arg1	with					1305:1308	with	1305:1308	with	1305:1308	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	20	80	theme	wall-degrading	2840:2853	arg1	enzymes					2855:2861	the plant cell wall-degrading enzymes	2825:2861	the plant cell wall-degrading enzymes	2825:2861	SirexAA-E responds to mannose and mannooligosaccharides through two different transcriptional repressors that regulate the secretion of the plant cell wall-degrading enzymes to extract carbon sources in the host environment.
33990299	6	81	theme	polysaccharide-degrading	726:749	arg1	ability					751:757	their polysaccharide-degrading ability	720:757	their polysaccharide-degrading ability	720:757	The secreted proteins from each culture supernatant were tested for their polysaccharide-degrading ability, and the composition of secreted CAZymes in each sample was determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
33990299	15	82	theme	bacterium	2123:2131	arg1	response					2106:2113	the response	2102:2113	the response of this bacterium to mannan	2102:2141	However, the response of this bacterium to mannan has not been documented.
33990299	3	83	theme	polysaccharides	311:325	arg1	degradation					288:298	the degradation	284:298	the degradation of various polysaccharides in the plant cell wall	284:348	strain SirexAA-E secretes a suite of carbohydrate-active enzymes (CAZymes), which are involved in the degradation of various polysaccharides in the plant cell wall, in response to the available carbon sources.
33990299	20	84	theme	cell	2835:2838	arg1	enzymes					2855:2861	the plant cell wall-degrading enzymes	2825:2861	the plant cell wall-degrading enzymes	2825:2861	SirexAA-E responds to mannose and mannooligosaccharides through two different transcriptional repressors that regulate the secretion of the plant cell wall-degrading enzymes to extract carbon sources in the host environment.
33990299	9	85	theme	SirexAA-E	1200:1208	arg1	genome					1210:1215	the SirexAA-E genome	1196:1215	the SirexAA-E genome	1196:1215	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	18	86	theme	cellobiose	2548:2557	arg1	SsCebR					2581:2586	SsCebR	2581:2586	SsCebR	2581:2586	We also demonstrated that the previously described cellobiose responsive regulator, SsCebR, could use mannobiose as an effector ligand.
33990299	18	86	theme	cellobiose	2548:2557	arg1	regulator					2570:2578	the previously described cellobiose responsive regulator	2523:2578	the previously described cellobiose responsive regulator	2523:2578	We also demonstrated that the previously described cellobiose responsive regulator, SsCebR, could use mannobiose as an effector ligand.
33990299	7	87	theme	cellulose-degrading	977:995	arg1	enzymes					997:1003	mannan and cellulose-degrading enzymes	966:1003	mannan and cellulose-degrading enzymes	966:1003	The results indicated that mannose, LBG, and CMC induced the secretion of mannan and cellulose-degrading enzymes.
33990299	3	88	theme	cell	340:343	arg1	wall					345:348	the plant cell wall	330:348	the plant cell wall	330:348	strain SirexAA-E secretes a suite of carbohydrate-active enzymes (CAZymes), which are involved in the degradation of various polysaccharides in the plant cell wall, in response to the available carbon sources.
33990299	7	89	theme	enzymes	997:1003	arg1	secretion					953:961	the secretion	949:961	the secretion of mannan and cellulose-degrading enzymes	949:1003	The results indicated that mannose, LBG, and CMC induced the secretion of mannan and cellulose-degrading enzymes.
33990299	5	90	theme	carboxymethyl	549:561	arg1	CMC					574:576	CMC	574:576	CMC	574:576	SirexAA-E grew well on mannose, carboxymethyl cellulose (CMC), and locust bean gum (LBG) as sole carbon sources in the culture medium.
33990299	5	90	theme	carboxymethyl	549:561	arg1	cellulose					563:571	carboxymethyl cellulose	549:571	carboxymethyl cellulose (CMC)	549:577	SirexAA-E grew well on mannose, carboxymethyl cellulose (CMC), and locust bean gum (LBG) as sole carbon sources in the culture medium.
33990299	20	91	theme	host	2896:2899	arg1	environment					2901:2911	the host environment	2892:2911	the host environment	2892:2911	SirexAA-E responds to mannose and mannooligosaccharides through two different transcriptional repressors that regulate the secretion of the plant cell wall-degrading enzymes to extract carbon sources in the host environment.
33990299	0	92	theme	Streptomyces	91:102	arg1	sp					104:105	the Insect-Associated Cellulolytic Bacterium Streptomyces sp	46:105	the Insect-Associated Cellulolytic Bacterium Streptomyces sp	46:105	Mannose- and Mannobiose-Specific Responses of the Insect-Associated Cellulolytic Bacterium Streptomyces sp.
33990299	4	93	theme	cell	495:498	arg1	components					505:514	the major plant cell wall components	479:514	the major plant cell wall components	479:514	Here, we examined a poorly understood response of this bacterium to mannan, one of the major plant cell wall components.
33990299	4	94	theme	components	505:514	arg1	one					472:474	one	472:474	one	472:474	Here, we examined a poorly understood response of this bacterium to mannan, one of the major plant cell wall components.
33990299	4	94	theme	components	505:514	arg1	mannan					464:469	mannan	464:469	mannan	464:469	Here, we examined a poorly understood response of this bacterium to mannan, one of the major plant cell wall components.
33990299	4	94	theme	components	505:514	arg1	components					505:514	the major plant cell wall components	479:514	the major plant cell wall components	479:514	Here, we examined a poorly understood response of this bacterium to mannan, one of the major plant cell wall components.
33990299	10	95	theme	cellobiose-responsive	1435:1455	arg1	SsCebR					1468:1473	SsCebR	1468:1473	SsCebR	1468:1473	Furthermore, the previously reported cellobiose-responsive repressor, SsCebR, was determined to also use mannobiose as an effector ligand.
33990299	10	95	theme	cellobiose-responsive	1435:1455	arg1	repressor					1457:1465	the previously reported cellobiose-responsive repressor	1411:1465	the previously reported cellobiose-responsive repressor	1411:1465	Furthermore, the previously reported cellobiose-responsive repressor, SsCebR, was determined to also use mannobiose as an effector ligand.
33990299	12	96	theme	SsManR	1888:1893	arg1	regulation					1869:1878	the regulation	1865:1878	the regulation of both SsManR and SsCebR	1865:1904	As hypothesized, the composition of the mannobiose secretome combined sets of CAZymes found in both LBG and CMC secretomes, and thus they are likely under the regulation of both SsManR and SsCebR.
33990299	3	97	theme	carbon	380:385	arg1	sources					387:393	the available carbon sources	366:393	the available carbon sources	366:393	strain SirexAA-E secretes a suite of carbohydrate-active enzymes (CAZymes), which are involved in the degradation of various polysaccharides in the plant cell wall, in response to the available carbon sources.
33990299	9	98	theme	α-1,2-mannosidase	1274:1290	arg1	genes					1292:1296	the above-mentioned α-1,2-mannosidase genes	1254:1296	the above-mentioned α-1,2-mannosidase genes	1254:1296	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	5	99	theme	bean	591:594	arg1	LBG					601:603	LBG	601:603	LBG	601:603	SirexAA-E grew well on mannose, carboxymethyl cellulose (CMC), and locust bean gum (LBG) as sole carbon sources in the culture medium.
33990299	5	99	theme	bean	591:594	arg1	gum					596:598	locust bean gum	584:598	locust bean gum (LBG)	584:604	SirexAA-E grew well on mannose, carboxymethyl cellulose (CMC), and locust bean gum (LBG) as sole carbon sources in the culture medium.
33990299	14	100	theme	insects	1988:1994	arg1	symbiont					1957:1964	a microbial symbiont	1945:1964	a microbial symbiont of biomass-harvesting insects	1945:1994	SirexAA-E, a microbial symbiont of biomass-harvesting insects, secretes a suite of polysaccharide-degrading enzymes dependent on the available carbon sources.
33990299	14	100	theme	insects	1988:1994	arg1	SirexAA-E					1934:1942	SirexAA-E	1934:1942	SirexAA-E	1934:1942	SirexAA-E, a microbial symbiont of biomass-harvesting insects, secretes a suite of polysaccharide-degrading enzymes dependent on the available carbon sources.
33990299	6	101	from	composition	768:778	arg1	sample					808:813	each sample	803:813	each sample	803:813	The secreted proteins from each culture supernatant were tested for their polysaccharide-degrading ability, and the composition of secreted CAZymes in each sample was determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
33990299	4	102	theme	major	483:487	arg1	components					505:514	the major plant cell wall components	479:514	the major plant cell wall components	479:514	Here, we examined a poorly understood response of this bacterium to mannan, one of the major plant cell wall components.
33990299	3	103	theme	enzymes	243:249	arg1	suite					214:218	a suite	212:218	a suite	212:218	strain SirexAA-E secretes a suite of carbohydrate-active enzymes (CAZymes), which are involved in the degradation of various polysaccharides in the plant cell wall, in response to the available carbon sources.
33990299	1	104	theme	Strain	108:113	arg1	SirexAA-E					115:123	Strain SirexAA-E	108:123	Strain SirexAA-E.	108:124	Strain SirexAA-E.
33990299	14	105	theme	available	2067:2075	arg1	sources					2084:2090	the available carbon sources	2063:2090	the available carbon sources	2063:2090	SirexAA-E, a microbial symbiont of biomass-harvesting insects, secretes a suite of polysaccharide-degrading enzymes dependent on the available carbon sources.
33990299	14	106	theme	microbial	1947:1955	arg1	symbiont					1957:1964	a microbial symbiont	1945:1964	a microbial symbiont of biomass-harvesting insects	1945:1994	SirexAA-E, a microbial symbiont of biomass-harvesting insects, secretes a suite of polysaccharide-degrading enzymes dependent on the available carbon sources.
33990299	14	106	theme	microbial	1947:1955	arg1	SirexAA-E					1934:1942	SirexAA-E	1934:1942	SirexAA-E	1934:1942	SirexAA-E, a microbial symbiont of biomass-harvesting insects, secretes a suite of polysaccharide-degrading enzymes dependent on the available carbon sources.
33990299	19	107	theme	Streptomyces	2672:2683	arg1	sp					2685:2686	the Streptomyces sp	2668:2686	the Streptomyces sp	2668:2686	Overall, our findings suggest that the Streptomyces sp.
33990299	14	108	theme	enzymes	2042:2048	arg1	suite					2008:2012	a suite	2006:2012	a suite of polysaccharide-degrading enzymes dependent on the available carbon sources	2006:2090	SirexAA-E, a microbial symbiont of biomass-harvesting insects, secretes a suite of polysaccharide-degrading enzymes dependent on the available carbon sources.
33990299	11	109	theme	genes	1584:1588	arg1	genes					1584:1588	genes	1584:1588	genes	1584:1588	To test whether mannobiose induces the sets of genes under the control of the two regulators, SirexAA-E was grown on mannobiose, and the secretome composition was analyzed.
33990299	11	109	theme	genes	1584:1588	arg1	sets					1576:1579	the sets	1572:1579	the sets of genes	1572:1588	To test whether mannobiose induces the sets of genes under the control of the two regulators, SirexAA-E was grown on mannobiose, and the secretome composition was analyzed.
33990299	0	110	theme	Cellulolytic	68:79	arg1	sp					104:105	the Insect-Associated Cellulolytic Bacterium Streptomyces sp	46:105	the Insect-Associated Cellulolytic Bacterium Streptomyces sp	46:105	Mannose- and Mannobiose-Specific Responses of the Insect-Associated Cellulolytic Bacterium Streptomyces sp.
33990299	5	111	from	sources	621:627	arg1	medium					644:649	the culture medium	632:649	the culture medium	632:649	SirexAA-E grew well on mannose, carboxymethyl cellulose (CMC), and locust bean gum (LBG) as sole carbon sources in the culture medium.
33990299	12	112	theme	CAZymes	1788:1794	arg1	sets					1780:1783	combined sets	1771:1783	combined sets of CAZymes found in both LBG and CMC secretomes	1771:1831	As hypothesized, the composition of the mannobiose secretome combined sets of CAZymes found in both LBG and CMC secretomes, and thus they are likely under the regulation of both SsManR and SsCebR.
33990299	12	112	theme	CAZymes	1788:1794	arg1	CAZymes					1788:1794	CAZymes	1788:1794	CAZymes found in both LBG and CMC secretomes	1788:1831	As hypothesized, the composition of the mannobiose secretome combined sets of CAZymes found in both LBG and CMC secretomes, and thus they are likely under the regulation of both SsManR and SsCebR.
33990299	17	113	theme	mannose	2362:2368	arg1	SsManR					2423:2428	SsManR	2423:2428	SsManR	2423:2428	By combining biochemical, proteomic, and genomic approaches, we discovered a novel mannose and mannobiose responsive transcriptional regulator, SsManR, which selectively regulates three α-1,2-mannosidase-coding genes.
33990299	17	113	theme	mannose	2362:2368	arg1	regulator					2412:2420	a novel mannose and mannobiose responsive transcriptional regulator	2354:2420	a novel mannose and mannobiose responsive transcriptional regulator	2354:2420	By combining biochemical, proteomic, and genomic approaches, we discovered a novel mannose and mannobiose responsive transcriptional regulator, SsManR, which selectively regulates three α-1,2-mannosidase-coding genes.
33990299	9	114	theme	mannobiose-responsive	1341:1361	arg1	SsManR					1390:1395	SsManR	1390:1395	SsManR	1390:1395	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	9	114	theme	mannobiose-responsive	1341:1361	arg1	regulator					1379:1387	a newly identified mannose and mannobiose-responsive transcriptional regulator	1310:1387	a newly identified mannose and mannobiose-responsive transcriptional regulator	1310:1387	Using genomic analysis, we found a unique 12-bp palindromic sequence motif at 4 locations in the SirexAA-E genome, two of which were found upstream of the above-mentioned α-1,2-mannosidase genes, along with a newly identified mannose and mannobiose-responsive transcriptional regulator, SsManR.
33990299	6	115	theme	CAZymes	792:798	arg1	composition					768:778	the composition	764:778	the composition of secreted CAZymes in each sample	764:813	The secreted proteins from each culture supernatant were tested for their polysaccharide-degrading ability, and the composition of secreted CAZymes in each sample was determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
33990299	17	116	theme	α-1,2-mannosidase-coding	2465:2488	arg1	genes					2490:2494	three α-1,2-mannosidase-coding genes	2459:2494	three α-1,2-mannosidase-coding genes	2459:2494	By combining biochemical, proteomic, and genomic approaches, we discovered a novel mannose and mannobiose responsive transcriptional regulator, SsManR, which selectively regulates three α-1,2-mannosidase-coding genes.
33990299	17	117	theme	biochemical	2292:2302	arg1	approaches					2328:2337	biochemical, proteomic, and genomic approaches	2292:2337	biochemical, proteomic, and genomic approaches	2292:2337	By combining biochemical, proteomic, and genomic approaches, we discovered a novel mannose and mannobiose responsive transcriptional regulator, SsManR, which selectively regulates three α-1,2-mannosidase-coding genes.
33990299	2	118	theme	bacterium	159:167	arg1	sp					182:183	The cellulolytic insect symbiont bacterium Streptomyces sp	126:183	The cellulolytic insect symbiont bacterium Streptomyces sp.	126:184	The cellulolytic insect symbiont bacterium Streptomyces sp.
34914909	5	0	dep	cytokines	1260:1268	arg1	VEGF					1278:1281	VEGF	1278:1281	VEGF	1278:1281	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	5	0	dep	cytokines	1260:1268	arg1	CD31					1288:1291	CD31	1288:1291	CD31	1288:1291	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	5	0	dep	cytokines	1260:1268	arg1	α-SMA					1271:1275	α-SMA	1271:1275	α-SMA	1271:1275	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	5	0	dep	cytokines	1260:1268	arg1	cytokines					1260:1268	angiogenesis-related cytokines	1239:1268	angiogenesis-related cytokines (α-SMA, VEGF, and CD31)	1239:1292	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	1	1	theme	wound	318:322	arg1	healing					324:330	bioactive wound healing	308:330	the bioactive wound healing substances in PA	304:347	In order to further investigate the bioactive wound healing substances in PA, crude PA protein-polysaccharide complexes were further purified by cellulose DE-52 and Sephadex G100 chromatography in succession.
34914909	3	2	theme	amino	698:702	arg1	acid					704:707	amino acid	698:707	amino acid	698:707	Mediated by HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis, the characterization of PaPPc2 and PaPPc3 was implemented.
34914909	0	3	from	healing	242:248	arg1	settings					262:269	clinical settings	253:269	clinical settings	253:269	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	3	4	theme	PaPPc3	862:867	arg1	characterization					831:846	the characterization	827:846	the characterization of PaPPc2 and PaPPc3	827:867	Mediated by HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis, the characterization of PaPPc2 and PaPPc3 was implemented.
34914909	3	5	theme	1H	810:811	arg1	NMR					813:815	1H NMR	810:815	1H NMR	810:815	Mediated by HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis, the characterization of PaPPc2 and PaPPc3 was implemented.
34914909	3	6	theme	HPGPC	691:695	arg1	analysis					817:824	HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis	691:824	HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis	691:824	Mediated by HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis, the characterization of PaPPc2 and PaPPc3 was implemented.
34914909	1	7	theme	healing	324:330	arg1	substances					332:341	the bioactive wound healing substances	304:341	the bioactive wound healing substances in PA	304:347	In order to further investigate the bioactive wound healing substances in PA, crude PA protein-polysaccharide complexes were further purified by cellulose DE-52 and Sephadex G100 chromatography in succession.
34914909	5	8	theme	blood	1206:1210	arg1	vessels					1212:1218	blood vessels	1206:1218	blood vessels	1206:1218	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	3	9	theme	NMR	813:815	arg1	analysis					817:824	HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis	691:824	HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis	691:824	Mediated by HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis, the characterization of PaPPc2 and PaPPc3 was implemented.
34914909	0	10	theme	ethno-medicine	113:126	arg1	americana					165:173	an animal ethno-medicine Periplaneta americana L. Periplaneta americana	103:173	an animal ethno-medicine Periplaneta americana L. Periplaneta americana L	103:175	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	2	11	theme	healing	658:664	arg1	experiments					666:676	the wound healing experiments	648:676	the wound healing experiments	648:676	Among these isolated fractions, two fractions eluted by 0.3 M and 0.5 M NaCl with the higher yield, respectively named PaPPc2 and PaPPc3 respectively, were chosen for the wound healing experiments.
34914909	3	12	theme	PaPPc2	851:856	arg1	characterization					831:846	the characterization	827:846	the characterization of PaPPc2 and PaPPc3	827:867	Mediated by HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis, the characterization of PaPPc2 and PaPPc3 was implemented.
34914909	6	13	theme	Periplaneta	1402:1412	arg1	americana					1414:1422	Periplaneta americana L.	1402:1425	Periplaneta americana L.	1402:1425	In short, this study provides an experimental basis to demonstrate the protein-polysaccharide complexes of Periplaneta americana L. as its wound healing bioactive substances.
34914909	0	14	theme	animal	106:111	arg1	americana					165:173	an animal ethno-medicine Periplaneta americana L. Periplaneta americana	103:173	an animal ethno-medicine Periplaneta americana L. Periplaneta americana L	103:175	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	3	15	theme	acid	704:707	arg1	analysis					740:747	amino acid and monosaccharide composition analysis	698:747	amino acid and monosaccharide composition analysis	698:747	Mediated by HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis, the characterization of PaPPc2 and PaPPc3 was implemented.
34914909	1	16	theme	Sephadex	437:444	arg1	chromatography					451:464	cellulose DE-52 and Sephadex G100 chromatography	417:464	chromatography	451:464	In order to further investigate the bioactive wound healing substances in PA, crude PA protein-polysaccharide complexes were further purified by cellulose DE-52 and Sephadex G100 chromatography in succession.
34914909	1	17	from	DE-52	427:431	arg1	succession					469:478	succession	469:478	succession	469:478	In order to further investigate the bioactive wound healing substances in PA, crude PA protein-polysaccharide complexes were further purified by cellulose DE-52 and Sephadex G100 chromatography in succession.
34914909	0	18	theme	americana	140:148	arg1	americana					165:173	an animal ethno-medicine Periplaneta americana L. Periplaneta americana	103:173	an animal ethno-medicine Periplaneta americana L. Periplaneta americana L	103:175	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	0	19	theme	medicine	201:208	arg1	type					186:189	a type	184:189	a type of animal medicine	184:208	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	0	19	theme	medicine	201:208	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	1	20	theme	G100	446:449	arg1	chromatography					451:464	cellulose DE-52 and Sephadex G100 chromatography	417:464	chromatography	451:464	In order to further investigate the bioactive wound healing substances in PA, crude PA protein-polysaccharide complexes were further purified by cellulose DE-52 and Sephadex G100 chromatography in succession.
34914909	6	21	theme	experimental	1328:1339	arg1	basis					1341:1345	an experimental basis	1325:1345	an experimental basis to demonstrate the protein-polysaccharide complexes of Periplaneta americana L. as its wound healing bioactive substances	1325:1467	In short, this study provides an experimental basis to demonstrate the protein-polysaccharide complexes of Periplaneta americana L. as its wound healing bioactive substances.
34914909	0	22	theme	Periplaneta	128:138	arg1	americana					165:173	an animal ethno-medicine Periplaneta americana L. Periplaneta americana	103:173	an animal ethno-medicine Periplaneta americana L. Periplaneta americana L	103:175	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	0	23	theme	animal	194:199	arg1	medicine					201:208	animal medicine	194:208	animal medicine	194:208	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	5	24	theme	mice	1178:1181	arg1	wounds					1159:1164	the acute wounds	1149:1164	the acute wounds of diabetic mice	1149:1181	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	4	25	theme	HUVECs	983:988	arg1	proliferation					935:947	cell proliferation	930:947	cell proliferation	930:947	And then, the benefits of PaPPcs to promote cell proliferation, migration, and tube formation of HUVECs were determined in vitro, indicated these fractions would facilitate angiogenesis.
34914909	4	25	theme	HUVECs	983:988	arg1	formation					970:978	tube formation	965:978	tube formation of HUVECs	965:988	And then, the benefits of PaPPcs to promote cell proliferation, migration, and tube formation of HUVECs were determined in vitro, indicated these fractions would facilitate angiogenesis.
34914909	4	25	theme	HUVECs	983:988	arg1	migration					950:958	migration	950:958	migration	950:958	And then, the benefits of PaPPcs to promote cell proliferation, migration, and tube formation of HUVECs were determined in vitro, indicated these fractions would facilitate angiogenesis.
34914909	1	26	theme	crude	350:354	arg1	complexes					382:390	crude PA protein-polysaccharide complexes	350:390	crude PA protein-polysaccharide complexes	350:390	In order to further investigate the bioactive wound healing substances in PA, crude PA protein-polysaccharide complexes were further purified by cellulose DE-52 and Sephadex G100 chromatography in succession.
34914909	0	27	theme	wound	236:240	arg1	healing					242:248	wound healing	236:248	wound healing in clinical settings	236:269	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	4	28	theme	PaPPcs	912:917	arg1	benefits					900:907	the benefits	896:907	the benefits of PaPPcs to promote cell proliferation, migration, and tube formation of HUVECs	896:988	And then, the benefits of PaPPcs to promote cell proliferation, migration, and tube formation of HUVECs were determined in vitro, indicated these fractions would facilitate angiogenesis.
34914909	3	29	theme	analysis	740:747	arg1	analysis					817:824	HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis	691:824	HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis	691:824	Mediated by HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis, the characterization of PaPPc2 and PaPPc3 was implemented.
34914909	0	30	theme	clinical	253:260	arg1	settings					262:269	clinical settings	253:269	clinical settings	253:269	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	4	31	dep	determined	995:1004	arg1	indicated					1016:1024	indicated	1016:1024	indicated these fractions would facilitate angiogenesis	1016:1070	And then, the benefits of PaPPcs to promote cell proliferation, migration, and tube formation of HUVECs were determined in vitro, indicated these fractions would facilitate angiogenesis.
34914909	5	32	theme	angiogenesis-related	1239:1258	arg1	VEGF					1278:1281	VEGF	1278:1281	VEGF	1278:1281	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	5	32	theme	angiogenesis-related	1239:1258	arg1	CD31					1288:1291	CD31	1288:1291	CD31	1288:1291	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	5	32	theme	angiogenesis-related	1239:1258	arg1	α-SMA					1271:1275	α-SMA	1271:1275	α-SMA	1271:1275	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	5	32	theme	angiogenesis-related	1239:1258	arg1	cytokines					1260:1268	angiogenesis-related cytokines	1239:1268	angiogenesis-related cytokines (α-SMA, VEGF, and CD31)	1239:1292	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	0	33	theme	wound	30:34	arg1	healing					36:42	diabetic wound healing	21:42	diabetic wound healing	21:42	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	2	34	theme	wound	652:656	arg1	healing					658:664	wound healing	652:664	the wound healing experiments	648:676	Among these isolated fractions, two fractions eluted by 0.3 M and 0.5 M NaCl with the higher yield, respectively named PaPPc2 and PaPPc3 respectively, were chosen for the wound healing experiments.
34914909	0	35	theme	Periplaneta	153:163	arg1	americana					165:173	an animal ethno-medicine Periplaneta americana L. Periplaneta americana	103:173	an animal ethno-medicine Periplaneta americana L. Periplaneta americana L	103:175	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	5	36	theme	cytokines	1260:1268	arg1	VEGF					1278:1281	VEGF	1278:1281	VEGF	1278:1281	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	5	36	theme	cytokines	1260:1268	arg1	vessels					1212:1218	blood vessels	1206:1218	blood vessels	1206:1218	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	5	36	theme	cytokines	1260:1268	arg1	cytokines					1260:1268	angiogenesis-related cytokines	1239:1268	angiogenesis-related cytokines (α-SMA, VEGF, and CD31)	1239:1292	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	5	36	theme	cytokines	1260:1268	arg1	CD31					1288:1291	CD31	1288:1291	CD31	1288:1291	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	5	36	theme	cytokines	1260:1268	arg1	α-SMA					1271:1275	α-SMA	1271:1275	α-SMA	1271:1275	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	5	36	theme	cytokines	1260:1268	arg1	amounts					1228:1234	the amounts	1224:1234	the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31)	1224:1292	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	0	37	theme	diabetic	21:28	arg1	healing					36:42	diabetic wound healing	21:42	diabetic wound healing	21:42	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	3	38	theme	monosaccharide	713:726	arg1	composition					728:738	monosaccharide composition	713:738	monosaccharide composition	713:738	Mediated by HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis, the characterization of PaPPc2 and PaPPc3 was implemented.
34914909	0	39	theme	L.	150:151	arg1	americana					165:173	an animal ethno-medicine Periplaneta americana L. Periplaneta americana	103:173	an animal ethno-medicine Periplaneta americana L. Periplaneta americana L	103:175	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	1	40	theme	PA	356:357	arg1	complexes					382:390	crude PA protein-polysaccharide complexes	350:390	crude PA protein-polysaccharide complexes	350:390	In order to further investigate the bioactive wound healing substances in PA, crude PA protein-polysaccharide complexes were further purified by cellulose DE-52 and Sephadex G100 chromatography in succession.
34914909	6	41	theme	bioactive	1448:1456	arg1	substances					1458:1467	its wound healing bioactive substances	1430:1467	its wound healing bioactive substances	1430:1467	In short, this study provides an experimental basis to demonstrate the protein-polysaccharide complexes of Periplaneta americana L. as its wound healing bioactive substances.
34914909	3	42	theme	composition	728:738	arg1	analysis					740:747	amino acid and monosaccharide composition analysis	698:747	amino acid and monosaccharide composition analysis	698:747	Mediated by HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis, the characterization of PaPPc2 and PaPPc3 was implemented.
34914909	1	43	theme	protein-polysaccharide	359:380	arg1	complexes					382:390	crude PA protein-polysaccharide complexes	350:390	crude PA protein-polysaccharide complexes	350:390	In order to further investigate the bioactive wound healing substances in PA, crude PA protein-polysaccharide complexes were further purified by cellulose DE-52 and Sephadex G100 chromatography in succession.
34914909	6	44	theme	healing	1440:1446	arg1	substances					1458:1467	its wound healing bioactive substances	1430:1467	its wound healing bioactive substances	1430:1467	In short, this study provides an experimental basis to demonstrate the protein-polysaccharide complexes of Periplaneta americana L. as its wound healing bioactive substances.
34914909	5	45	theme	acute	1153:1157	arg1	wounds					1159:1164	the acute wounds	1149:1164	the acute wounds of diabetic mice	1149:1181	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	1	46	from	substances	332:341	arg1	PA					346:347	PA	346:347	PA	346:347	In order to further investigate the bioactive wound healing substances in PA, crude PA protein-polysaccharide complexes were further purified by cellulose DE-52 and Sephadex G100 chromatography in succession.
34914909	3	47	theme	spectrum	769:776	arg1	analysis					817:824	HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis	691:824	HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis	691:824	Mediated by HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis, the characterization of PaPPc2 and PaPPc3 was implemented.
34914909	0	48	theme	americana	165:173	arg1	L					175:175	an animal ethno-medicine Periplaneta americana L. Periplaneta americana L	103:175	an animal ethno-medicine Periplaneta americana L. Periplaneta americana L	103:175	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	0	49	theme	protein-polysaccharide	56:77	arg1	complexes					79:87	protein-polysaccharide complexes	56:87	protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L	56:175	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	2	50	dep	0.3 M	537:541	arg1	NaCl					553:556	NaCl	553:556	NaCl	553:556	Among these isolated fractions, two fractions eluted by 0.3 M and 0.5 M NaCl with the higher yield, respectively named PaPPc2 and PaPPc3 respectively, were chosen for the wound healing experiments.
34914909	2	51	theme	higher	567:572	arg1	yield					574:578	the higher yield	563:578	the higher yield	563:578	Among these isolated fractions, two fractions eluted by 0.3 M and 0.5 M NaCl with the higher yield, respectively named PaPPc2 and PaPPc3 respectively, were chosen for the wound healing experiments.
34914909	2	52	theme	isolated	493:500	arg1	fractions					502:510	these isolated fractions	487:510	these isolated fractions	487:510	Among these isolated fractions, two fractions eluted by 0.3 M and 0.5 M NaCl with the higher yield, respectively named PaPPc2 and PaPPc3 respectively, were chosen for the wound healing experiments.
34914909	0	53	attach	isolated	89:96	arg2	complexes					79:87	protein-polysaccharide complexes	56:87	protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L	56:175	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	0	53	attach	isolated	89:96	arg1	L					175:175	an animal ethno-medicine Periplaneta americana L. Periplaneta americana L	103:175	an animal ethno-medicine Periplaneta americana L. Periplaneta americana L	103:175	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	0	54	used	used	227:230	arg2	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	0	54	used	used	227:230	arg2	PA					179:180	PA	179:180	PA	179:180	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	0	54	used	used	227:230	arg2	healing					36:42	diabetic wound healing	21:42	diabetic wound healing	21:42	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	0	54	used	used	227:230	arg2	type					186:189	a type	184:189	a type of animal medicine	184:208	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	4	55	theme	tube	965:968	arg1	formation					970:978	tube formation	965:978	tube formation of HUVECs	965:988	And then, the benefits of PaPPcs to promote cell proliferation, migration, and tube formation of HUVECs were determined in vitro, indicated these fractions would facilitate angiogenesis.
34914909	3	56	theme	circular	750:757	arg1	spectrum					769:776	circular dichroism spectrum	750:776	circular dichroism spectrum	750:776	Mediated by HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis, the characterization of PaPPc2 and PaPPc3 was implemented.
34914909	4	57	theme	cell	930:933	arg1	proliferation					935:947	cell proliferation	930:947	cell proliferation	930:947	And then, the benefits of PaPPcs to promote cell proliferation, migration, and tube formation of HUVECs were determined in vitro, indicated these fractions would facilitate angiogenesis.
34914909	6	58	dep	americana	1414:1422	arg1	L.					1424:1425	Periplaneta americana L.	1402:1425	Periplaneta americana L.	1402:1425	In short, this study provides an experimental basis to demonstrate the protein-polysaccharide complexes of Periplaneta americana L. as its wound healing bioactive substances.
34914909	6	59	theme	wound	1434:1438	arg1	healing					1440:1446	its wound healing	1430:1446	its wound healing bioactive substances	1430:1467	In short, this study provides an experimental basis to demonstrate the protein-polysaccharide complexes of Periplaneta americana L. as its wound healing bioactive substances.
34914909	3	60	theme	dichroism	759:767	arg1	spectrum					769:776	circular dichroism spectrum	750:776	circular dichroism spectrum	750:776	Mediated by HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis, the characterization of PaPPc2 and PaPPc3 was implemented.
34914909	1	61	from	chromatography	451:464	arg1	succession					469:478	succession	469:478	succession	469:478	In order to further investigate the bioactive wound healing substances in PA, crude PA protein-polysaccharide complexes were further purified by cellulose DE-52 and Sephadex G100 chromatography in succession.
34914909	5	62	theme	diabetic	1169:1176	arg1	mice					1178:1181	diabetic mice	1169:1181	diabetic mice	1169:1181	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	3	63	theme	FT-IR	799:803	arg1	analysis					817:824	HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis	691:824	HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis	691:824	Mediated by HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis, the characterization of PaPPc2 and PaPPc3 was implemented.
34914909	1	64	theme	cellulose	417:425	arg1	DE-52					427:431	cellulose DE-52 and Sephadex G100 chromatography	417:464	DE-52	427:431	In order to further investigate the bioactive wound healing substances in PA, crude PA protein-polysaccharide complexes were further purified by cellulose DE-52 and Sephadex G100 chromatography in succession.
34914909	5	65	theme	concept	1094:1100	arg1	proof					1085:1089	proof	1085:1089	proof of concept	1085:1100	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	5	65	theme	concept	1094:1100	arg1	PaPPc3					1114:1119	PaPPc3	1114:1119	PaPPc3	1114:1119	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	5	65	theme	concept	1094:1100	arg1	PaPPc2					1103:1108	PaPPc2	1103:1108	PaPPc2	1103:1108	Finally, as proof of concept, PaPPc2 and PaPPc3 were employed to accelerate the acute wounds of diabetic mice, involving in increase blood vessels and the amounts of angiogenesis-related cytokines (α-SMA, VEGF, and CD31).
34914909	3	66	theme	glycosylation	779:791	arg1	type					793:796	glycosylation type	779:796	glycosylation type	779:796	Mediated by HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis, the characterization of PaPPc2 and PaPPc3 was implemented.
34914909	0	67	theme	complexes	79:87	arg1	type					186:189	a type	184:189	a type of animal medicine	184:208	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	0	67	theme	complexes	79:87	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	0	67	theme	complexes	79:87	arg1	PA					179:180	PA	179:180	PA	179:180	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	0	67	theme	complexes	79:87	arg1	healing					36:42	diabetic wound healing	21:42	diabetic wound healing	21:42	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	1	68	theme	bioactive	308:316	arg1	healing					324:330	bioactive wound healing	308:330	the bioactive wound healing substances in PA	304:347	In order to further investigate the bioactive wound healing substances in PA, crude PA protein-polysaccharide complexes were further purified by cellulose DE-52 and Sephadex G100 chromatography in succession.
34914909	0	69	dep	Characterization	0:15	arg1	benefits					44:51	benefits	44:51	benefits	44:51	Characterization and diabetic wound healing benefits of protein-polysaccharide complexes isolated from an animal ethno-medicine Periplaneta americana L. Periplaneta americana L. (PA), a type of animal medicine, has been widely used for wound healing in clinical settings.
34914909	6	70	theme	protein-polysaccharide	1366:1387	arg1	complexes					1389:1397	the protein-polysaccharide complexes	1362:1397	the protein-polysaccharide complexes of Periplaneta americana L. as its wound healing bioactive substances	1362:1467	In short, this study provides an experimental basis to demonstrate the protein-polysaccharide complexes of Periplaneta americana L. as its wound healing bioactive substances.
34914909	6	71	theme	americana	1414:1422	arg1	complexes					1389:1397	the protein-polysaccharide complexes	1362:1397	the protein-polysaccharide complexes of Periplaneta americana L. as its wound healing bioactive substances	1362:1467	In short, this study provides an experimental basis to demonstrate the protein-polysaccharide complexes of Periplaneta americana L. as its wound healing bioactive substances.
34914909	3	72	theme	type	793:796	arg1	analysis					817:824	HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis	691:824	HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis	691:824	Mediated by HPGPC, amino acid and monosaccharide composition analysis, circular dichroism spectrum, glycosylation type, FT-IR, and 1H NMR analysis, the characterization of PaPPc2 and PaPPc3 was implemented.
33739203	4	0	theme	CS	925:926	arg1	ones					976:979	the ones	972:979	especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA	961:1028	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	4	0	theme	CS	925:926	arg1	scaffolds					950:958	the CS and HA-based hydrogel scaffolds	921:958	the CS and HA-based hydrogel scaffolds	921:958	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	3	1	theme	hydrogels	727:735	arg1	studies					692:698	Mechanical studies	681:698	Mechanical studies of CS/HA and CS/HA/Si-HPMC hydrogels	681:735	Mechanical studies of CS/HA and CS/HA/Si-HPMC hydrogels showed that as Si-HPMC content increased, swelling rate and rheological properties were higher, compressive strength decreased and degradation was faster.
33739203	8	2	theme	mechanical	1965:1974	arg1	hydrogel					2005:2012	mechanical properties.The CS/HA/Si-HPMC hydrogel	1965:2012	mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation	1965:2064	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	2	3	theme	composite	371:379	arg1	hydrogels					381:389	composite hydrogels	371:389	composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC)	371:521	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	5	4	theme	hydrogels	1218:1226	arg1	studies					1183:1189	In vitro studies	1174:1189	In vitro studies of CS/HA and CS/HA/Si-HPMC hydrogels encapsulated in chondrocytes	1174:1255	In vitro studies of CS/HA and CS/HA/Si-HPMC hydrogels encapsulated in chondrocytes have shown that the proper amount of Si-HPMC increases the proliferation and deposition of the cartilage extracellular matrix.
33739203	4	5	theme	HA-based	932:939	arg1	ones					976:979	the ones	972:979	especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA	961:1028	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	4	5	theme	HA-based	932:939	arg1	scaffolds					950:958	the CS and HA-based hydrogel scaffolds	921:958	the CS and HA-based hydrogel scaffolds	921:958	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	5	6	dep	proliferation	1316:1328	arg1	the					1312:1314	the	1312:1314	the	1312:1314	In vitro studies of CS/HA and CS/HA/Si-HPMC hydrogels encapsulated in chondrocytes have shown that the proper amount of Si-HPMC increases the proliferation and deposition of the cartilage extracellular matrix.
33739203	4	7	theme	bioactive	1070:1078	arg1	properties					1080:1089	bioactive properties	1070:1089	bioactive properties	1070:1089	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	8	8	theme	principle	1686:1694	arg1	proof					1673:1677	proof	1673:1677	proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering	1673:2252	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	8	9	with	rheological	1948:1958	arg1	hydrogel					2005:2012	mechanical properties.The CS/HA/Si-HPMC hydrogel	1965:2012	mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation	1965:2064	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	8	10	theme	CS/HA/Si-HPMC	1991:2003	arg1	hydrogel					2005:2012	mechanical properties.The CS/HA/Si-HPMC hydrogel	1965:2012	mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation	1965:2064	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	8	11	theme	repair	1752:1757	arg1	cartilage					1765:1773	repair joint cartilage	1752:1773	repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering	1752:2252	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	2	12	theme	crosslinking	661:672	arg1	agent					674:678	a chemical crosslinking agent	650:678	a chemical crosslinking agent	650:678	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	3	13	theme	Si-HPMC	752:758	arg1	content					760:766	Si-HPMC content	752:766	Si-HPMC content	752:766	Mechanical studies of CS/HA and CS/HA/Si-HPMC hydrogels showed that as Si-HPMC content increased, swelling rate and rheological properties were higher, compressive strength decreased and degradation was faster.
33739203	4	14	theme	cartilage	1144:1152	arg1	engineering					1161:1171	cartilage tissue engineering	1144:1171	cartilage tissue engineering	1144:1171	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	8	15	theme	CS/HA/Si-HPMC	2175:2187	arg1	hydrogels					2189:2197	CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels	2140:2197	CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels	2140:2197	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	4	16	contain	have	1031:1034	arg2	performance					1054:1064	suitable physical performance	1036:1064	suitable physical performance	1036:1064	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	4	16	contain	have	1031:1034	arg2	properties					1080:1089	bioactive properties	1070:1089	bioactive properties	1070:1089	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	4	16	contain	have	1031:1034	arg1	scaffolds					950:958	the CS and HA-based hydrogel scaffolds	921:958	the CS and HA-based hydrogel scaffolds	921:958	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	4	16	contain	have	1031:1034	arg1	ones					976:979	the ones	972:979	especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA	961:1028	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	4	17	theme	potential	1107:1115	arg1	opportunity					1117:1127	a potential opportunity	1105:1127	a potential opportunity to be used for cartilage tissue engineering	1105:1171	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	3	18	theme	swelling	779:786	arg1	rate					788:791	swelling rate	779:791	swelling rate	779:791	Mechanical studies of CS/HA and CS/HA/Si-HPMC hydrogels showed that as Si-HPMC content increased, swelling rate and rheological properties were higher, compressive strength decreased and degradation was faster.
33739203	8	19	theme	high	2036:2039	arg1	swelling					2041:2048	high swelling	2036:2048	high swelling	2036:2048	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	6	20	theme	in vivo	1527:1533	arg1	regeneration					1540:1551	relatively good in vivo bone regeneration	1511:1551	relatively good in vivo bone regeneration	1511:1551	The regeneration rate of the CS/HA/Si-HPMC (3%) hydrogel reached about 79.5% at 21 days for long retention periods, indicating relatively good in vivo bone regeneration.
33739203	4	21	theme	3.0	986:988	arg1	%					989:989	%	989:989	%	989:989	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	4	22	dep	%	989:989	arg1	w/v					992:994	w/v	992:994	w/v	992:994	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	8	23	contain	has	1724:1726	arg2	ability					1741:1747	an excellent ability	1728:1747	an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering	1728:2252	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	8	23	contain	has	1724:1726	arg1	hydrogel					1715:1722	the resulting hydrogel	1701:1722	the resulting hydrogel	1701:1722	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	8	24	theme	injectable	1832:1841	arg1	hydrogel					1843:1850	a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel	1781:1850	a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering	1781:2252	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	8	25	theme	ratio.Increasing	2066:2081	arg1	concentration					2083:2095	the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration	1936:2095	the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC	1936:2106	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	2	26	theme	tissue	593:598	arg1	engineering					600:610	cartilage tissue engineering	583:610	cartilage tissue engineering	583:610	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	8	27	theme	CS/HA/Si-HPMC	1861:1873	arg1	composites					1875:1884	CS/HA/Si-HPMC composites	1861:1884	CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering	1861:2252	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	3	28	theme	compressive	833:843	arg1	strength					845:852	compressive strength	833:852	compressive strength	833:852	Mechanical studies of CS/HA and CS/HA/Si-HPMC hydrogels showed that as Si-HPMC content increased, swelling rate and rheological properties were higher, compressive strength decreased and degradation was faster.
33739203	0	29	theme	silanized-hydroxypropyl	73:95	arg1	methylcellulose					97:111	silanized-hydroxypropyl methylcellulose	73:111	silanized-hydroxypropyl methylcellulose as an injectable	73:128	Structural and biological investigation of chitosan/hyaluronic acid with silanized-hydroxypropyl methylcellulose as an injectable reinforced interpenetrating network hydrogel for cartilage tissue engineering.
33739203	7	30	theme	compatibility	1620:1632	arg1	scaffolds					1645:1653	tissue compatibility injectable scaffolds	1613:1653	tissue compatibility injectable scaffolds	1613:1653	These CS/HA/Si-HPMC hydrogels are promising candidates for tissue compatibility injectable scaffolds.
33739203	4	31	theme	%	1016:1016	arg1	CS/HA					1024:1028	2.5/4.0% (w/v) CS/HA	1009:1028	2.5/4.0% (w/v) CS/HA	1009:1028	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	5	32	theme	proper	1277:1282	arg1	amount					1284:1289	the proper amount	1273:1289	the proper amount of Si-HPMC	1273:1300	In vitro studies of CS/HA and CS/HA/Si-HPMC hydrogels encapsulated in chondrocytes have shown that the proper amount of Si-HPMC increases the proliferation and deposition of the cartilage extracellular matrix.
33739203	5	32	theme	proper	1277:1282	arg1	Si-HPMC					1294:1300	Si-HPMC	1294:1300	Si-HPMC	1294:1300	In vitro studies of CS/HA and CS/HA/Si-HPMC hydrogels encapsulated in chondrocytes have shown that the proper amount of Si-HPMC increases the proliferation and deposition of the cartilage extracellular matrix.
33739203	8	33	theme	organized	2119:2127	arg1	network					2129:2135	an organized network	2116:2135	an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering	2116:2252	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	8	34	theme	CS/HA/Si-HPMC	1904:1916	arg1	hydrogel					1918:1925	developed.The CS/HA/Si-HPMC hydrogel	1890:1925	developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering	1890:2252	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	8	35	dep	hydrogel	2005:2012	arg1	porous					2024:2029	porous	2024:2029	porous	2024:2029	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	7	36	theme	promising	1588:1596	arg1	candidates					1598:1607	promising candidates	1588:1607	promising candidates for tissue compatibility injectable scaffolds	1588:1653	These CS/HA/Si-HPMC hydrogels are promising candidates for tissue compatibility injectable scaffolds.
33739203	7	36	theme	promising	1588:1596	arg1	hydrogels					1574:1582	These CS/HA/Si-HPMC hydrogels	1554:1582	These CS/HA/Si-HPMC hydrogels	1554:1582	These CS/HA/Si-HPMC hydrogels are promising candidates for tissue compatibility injectable scaffolds.
33739203	2	37	theme	agent	674:678	arg1	addition					638:645	the addition	634:645	the addition of a chemical crosslinking agent	634:678	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	5	38	theme	extracellular	1362:1374	arg1	matrix					1376:1381	the cartilage extracellular matrix	1348:1381	the cartilage extracellular matrix	1348:1381	In vitro studies of CS/HA and CS/HA/Si-HPMC hydrogels encapsulated in chondrocytes have shown that the proper amount of Si-HPMC increases the proliferation and deposition of the cartilage extracellular matrix.
33739203	0	39	theme	interpenetrating	141:156	arg1	hydrogel					166:173	interpenetrating network hydrogel	141:173	interpenetrating network hydrogel for cartilage tissue engineering	141:206	Structural and biological investigation of chitosan/hyaluronic acid with silanized-hydroxypropyl methylcellulose as an injectable reinforced interpenetrating network hydrogel for cartilage tissue engineering.
33739203	8	40	theme	tunable	1940:1946	arg1	concentration					2083:2095	the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration	1936:2095	the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC	1936:2106	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	4	41	with	ones	976:979	arg1	CS/HA					1024:1028	2.5/4.0% (w/v) CS/HA	1009:1028	2.5/4.0% (w/v) CS/HA	1009:1028	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	4	41	with	ones	976:979	arg1	Si-HPMC					997:1003	3.0% (w/v) Si-HPMC	986:1003	3.0% (w/v) Si-HPMC	986:1003	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	2	42	theme	silanized-hydroxypropyl	457:479	arg1	Si-HPMC					498:504	Si-HPMC	498:504	Si-HPMC	498:504	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	2	42	theme	silanized-hydroxypropyl	457:479	arg1	CS/HA/Si-HPMC					508:520	CS/HA/Si-HPMC	508:520	CS/HA/Si-HPMC	508:520	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	2	42	theme	silanized-hydroxypropyl	457:479	arg1	methylcellulose					481:495	silanized-hydroxypropyl methylcellulose	457:495	silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC)	457:521	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	0	43	theme	Structural	0:9	arg1	investigation					26:38	Structural and biological investigation	0:38	Structural and biological investigation of chitosan/hyaluronic acid with silanized-hydroxypropyl methylcellulose as an injectable	0:128	Structural and biological investigation of chitosan/hyaluronic acid with silanized-hydroxypropyl methylcellulose as an injectable reinforced interpenetrating network hydrogel for cartilage tissue engineering.
33739203	8	44	dep	network	2129:2135	arg1	have					2199:2202	have	2199:2202	have a high potential for cartilage tissue engineering	2199:2252	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	4	45	theme	physical	1045:1052	arg1	performance					1054:1064	suitable physical performance	1036:1064	suitable physical performance	1036:1064	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	8	46	with	porous	2024:2029	arg1	degradation					2054:2064	degradation	2054:2064	degradation	2054:2064	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	8	46	with	porous	2024:2029	arg1	swelling					2041:2048	high swelling	2036:2048	high swelling	2036:2048	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	0	47	theme	biological	15:24	arg1	investigation					26:38	Structural and biological investigation	0:38	Structural and biological investigation of chitosan/hyaluronic acid with silanized-hydroxypropyl methylcellulose as an injectable	0:128	Structural and biological investigation of chitosan/hyaluronic acid with silanized-hydroxypropyl methylcellulose as an injectable reinforced interpenetrating network hydrogel for cartilage tissue engineering.
33739203	5	48	theme	CS/HA	1194:1198	arg1	hydrogels					1218:1226	CS/HA and CS/HA/Si-HPMC hydrogels	1194:1226	CS/HA and CS/HA/Si-HPMC hydrogels encapsulated in chondrocytes	1194:1255	In vitro studies of CS/HA and CS/HA/Si-HPMC hydrogels encapsulated in chondrocytes have shown that the proper amount of Si-HPMC increases the proliferation and deposition of the cartilage extracellular matrix.
33739203	7	49	theme	CS/HA/Si-HPMC	1560:1572	arg1	candidates					1598:1607	promising candidates	1588:1607	promising candidates for tissue compatibility injectable scaffolds	1588:1653	These CS/HA/Si-HPMC hydrogels are promising candidates for tissue compatibility injectable scaffolds.
33739203	7	49	theme	CS/HA/Si-HPMC	1560:1572	arg1	hydrogels					1574:1582	These CS/HA/Si-HPMC hydrogels	1554:1582	These CS/HA/Si-HPMC hydrogels	1554:1582	These CS/HA/Si-HPMC hydrogels are promising candidates for tissue compatibility injectable scaffolds.
33739203	2	50	theme	/hyaluronic	431:441	arg1	HA					449:450	HA	449:450	HA	449:450	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	2	50	theme	/hyaluronic	431:441	arg1	acid					443:446	water-soluble chitosan (CS)/hyaluronic acid	404:446	water-soluble chitosan (CS)/hyaluronic acid (HA)	404:451	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	6	51	theme	hydrogel	1432:1439	arg1	rate					1401:1404	The regeneration rate	1384:1404	The regeneration rate of the CS/HA/Si-HPMC (3%) hydrogel	1384:1439	The regeneration rate of the CS/HA/Si-HPMC (3%) hydrogel reached about 79.5% at 21 days for long retention periods, indicating relatively good in vivo bone regeneration.
33739203	0	52	theme	acid	63:66	arg1	investigation					26:38	Structural and biological investigation	0:38	Structural and biological investigation of chitosan/hyaluronic acid with silanized-hydroxypropyl methylcellulose as an injectable	0:128	Structural and biological investigation of chitosan/hyaluronic acid with silanized-hydroxypropyl methylcellulose as an injectable reinforced interpenetrating network hydrogel for cartilage tissue engineering.
33739203	5	53	theme	In	1174:1175	arg1	studies					1183:1189	In vitro studies	1174:1189	In vitro studies of CS/HA and CS/HA/Si-HPMC hydrogels encapsulated in chondrocytes	1174:1255	In vitro studies of CS/HA and CS/HA/Si-HPMC hydrogels encapsulated in chondrocytes have shown that the proper amount of Si-HPMC increases the proliferation and deposition of the cartilage extracellular matrix.
33739203	3	54	theme	CS/HA/Si-HPMC	713:725	arg1	hydrogels					727:735	CS/HA/Si-HPMC hydrogels	713:735	CS/HA/Si-HPMC hydrogels	713:735	Mechanical studies of CS/HA and CS/HA/Si-HPMC hydrogels showed that as Si-HPMC content increased, swelling rate and rheological properties were higher, compressive strength decreased and degradation was faster.
33739203	2	55	theme	new	358:360	arg1	class					362:366	a new class	356:366	a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC)	356:521	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	2	55	theme	new	358:360	arg1	hydrogels					569:577	injectable hydrogels	558:577	injectable hydrogels for cartilage tissue engineering	558:610	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	3	56	theme	CS/HA	703:707	arg1	studies					692:698	Mechanical studies	681:698	Mechanical studies of CS/HA and CS/HA/Si-HPMC hydrogels	681:735	Mechanical studies of CS/HA and CS/HA/Si-HPMC hydrogels showed that as Si-HPMC content increased, swelling rate and rheological properties were higher, compressive strength decreased and degradation was faster.
33739203	8	57	theme	resulting	1705:1713	arg1	hydrogel					1715:1722	the resulting hydrogel	1701:1722	the resulting hydrogel	1701:1722	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	2	58	attach	derived	391:397	arg1	HA					449:450	HA	449:450	HA	449:450	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	2	58	attach	derived	391:397	arg1	CS/HA/Si-HPMC					508:520	CS/HA/Si-HPMC	508:520	CS/HA/Si-HPMC	508:520	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	2	58	attach	derived	391:397	arg2	hydrogels					381:389	composite hydrogels	371:389	composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC)	371:521	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	2	58	attach	derived	391:397	arg1	acid					443:446	water-soluble chitosan (CS)/hyaluronic acid	404:446	water-soluble chitosan (CS)/hyaluronic acid (HA)	404:451	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	2	58	attach	derived	391:397	arg1	methylcellulose					481:495	silanized-hydroxypropyl methylcellulose	457:495	silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC)	457:521	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	2	58	attach	derived	391:397	arg1	Si-HPMC					498:504	Si-HPMC	498:504	Si-HPMC	498:504	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	8	59	contain	have	2199:2202	arg1	hydrogels					2189:2197	CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels	2140:2197	CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels	2140:2197	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	8	59	contain	have	2199:2202	arg2	potential					2211:2219	a high potential	2204:2219	a high potential for cartilage tissue engineering	2204:2252	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	8	60	theme	properties.The	1976:1989	arg1	hydrogel					2005:2012	mechanical properties.The CS/HA/Si-HPMC hydrogel	1965:2012	mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation	1965:2064	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	2	61	theme	hydrogels	381:389	arg1	class					362:366	a new class	356:366	a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC)	356:521	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	2	61	theme	hydrogels	381:389	arg1	hydrogels					569:577	injectable hydrogels	558:577	injectable hydrogels for cartilage tissue engineering	558:610	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	6	62	theme	long	1476:1479	arg1	periods					1491:1497	long retention periods	1476:1497	long retention periods	1476:1497	The regeneration rate of the CS/HA/Si-HPMC (3%) hydrogel reached about 79.5% at 21 days for long retention periods, indicating relatively good in vivo bone regeneration.
33739203	5	63	theme	CS/HA/Si-HPMC	1204:1216	arg1	hydrogels					1218:1226	CS/HA and CS/HA/Si-HPMC hydrogels	1194:1226	CS/HA and CS/HA/Si-HPMC hydrogels encapsulated in chondrocytes	1194:1255	In vitro studies of CS/HA and CS/HA/Si-HPMC hydrogels encapsulated in chondrocytes have shown that the proper amount of Si-HPMC increases the proliferation and deposition of the cartilage extracellular matrix.
33739203	8	64	theme	excellent	1731:1739	arg1	ability					1741:1747	an excellent ability	1728:1747	an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering	1728:2252	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	8	65	theme	cartilage	2225:2233	arg1	engineering					2242:2252	cartilage tissue engineering	2225:2252	cartilage tissue engineering	2225:2252	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	2	66	theme	chemical	652:659	arg1	agent					674:678	a chemical crosslinking agent	650:678	a chemical crosslinking agent	650:678	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	4	67	theme	tissue	1154:1159	arg1	engineering					1161:1171	cartilage tissue engineering	1144:1171	cartilage tissue engineering	1144:1171	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	4	68	dep	have	1031:1034	arg1	provide					1097:1103	provide	1097:1103	provide a potential opportunity to be used for cartilage tissue engineering	1097:1171	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	8	69	theme	joint	1759:1763	arg1	cartilage					1765:1773	repair joint cartilage	1752:1773	repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering	1752:2252	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	3	70	theme	rheological	797:807	arg1	properties					809:818	rheological properties	797:818	rheological properties	797:818	Mechanical studies of CS/HA and CS/HA/Si-HPMC hydrogels showed that as Si-HPMC content increased, swelling rate and rheological properties were higher, compressive strength decreased and degradation was faster.
33739203	6	71	theme	bone	1535:1538	arg1	regeneration					1540:1551	relatively good in vivo bone regeneration	1511:1551	relatively good in vivo bone regeneration	1511:1551	The regeneration rate of the CS/HA/Si-HPMC (3%) hydrogel reached about 79.5% at 21 days for long retention periods, indicating relatively good in vivo bone regeneration.
33739203	0	72	theme	tissue	189:194	arg1	engineering					196:206	cartilage tissue engineering	179:206	cartilage tissue engineering	179:206	Structural and biological investigation of chitosan/hyaluronic acid with silanized-hydroxypropyl methylcellulose as an injectable reinforced interpenetrating network hydrogel for cartilage tissue engineering.
33739203	6	73	theme	good	1522:1525	arg1	regeneration					1540:1551	relatively good in vivo bone regeneration	1511:1551	relatively good in vivo bone regeneration	1511:1551	The regeneration rate of the CS/HA/Si-HPMC (3%) hydrogel reached about 79.5% at 21 days for long retention periods, indicating relatively good in vivo bone regeneration.
33739203	4	74	dep	%	1016:1016	arg1	w/v					1019:1021	w/v	1019:1021	w/v	1019:1021	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	8	75	theme	HIGHLIGHTSAn	1819:1830	arg1	hydrogel					1843:1850	a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel	1781:1850	a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering	1781:2252	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	4	76	theme	hydrogel	941:948	arg1	ones					976:979	the ones	972:979	especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA	961:1028	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	4	76	theme	hydrogel	941:948	arg1	scaffolds					950:958	the CS and HA-based hydrogel scaffolds	921:958	the CS and HA-based hydrogel scaffolds	921:958	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	5	77	theme	Si-HPMC	1294:1300	arg1	amount					1284:1289	the proper amount	1273:1289	the proper amount of Si-HPMC	1273:1300	In vitro studies of CS/HA and CS/HA/Si-HPMC hydrogels encapsulated in chondrocytes have shown that the proper amount of Si-HPMC increases the proliferation and deposition of the cartilage extracellular matrix.
33739203	5	77	theme	Si-HPMC	1294:1300	arg1	Si-HPMC					1294:1300	Si-HPMC	1294:1300	Si-HPMC	1294:1300	In vitro studies of CS/HA and CS/HA/Si-HPMC hydrogels encapsulated in chondrocytes have shown that the proper amount of Si-HPMC increases the proliferation and deposition of the cartilage extracellular matrix.
33739203	8	78	theme	Si-HPMC	2100:2106	arg1	concentration					2083:2095	the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration	1936:2095	the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC	1936:2106	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	7	79	theme	injectable	1634:1643	arg1	scaffolds					1645:1653	tissue compatibility injectable scaffolds	1613:1653	tissue compatibility injectable scaffolds	1613:1653	These CS/HA/Si-HPMC hydrogels are promising candidates for tissue compatibility injectable scaffolds.
33739203	2	80	theme	cartilage	583:591	arg1	engineering					600:610	cartilage tissue engineering	583:610	cartilage tissue engineering	583:610	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	8	81	dep	hydrogel	1918:1925	arg1	displays					1927:1934	displays	1927:1934	displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering	1927:2252	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	7	82	theme	tissue	1613:1618	arg1	scaffolds					1645:1653	tissue compatibility injectable scaffolds	1613:1653	tissue compatibility injectable scaffolds	1613:1653	These CS/HA/Si-HPMC hydrogels are promising candidates for tissue compatibility injectable scaffolds.
33739203	6	83	theme	CS/HA/Si-HPMC	1413:1425	arg1	hydrogel					1432:1439	the CS/HA/Si-HPMC (3%) hydrogel	1409:1439	the CS/HA/Si-HPMC (3%) hydrogel	1409:1439	The regeneration rate of the CS/HA/Si-HPMC (3%) hydrogel reached about 79.5% at 21 days for long retention periods, indicating relatively good in vivo bone regeneration.
33739203	1	84	theme	Cartilage	209:217	arg1	damage					219:224	Cartilage damage	209:224	Cartilage damage	209:224	Cartilage damage continues to pose a threat to humans, but no treatment is currently available to fully restore cartilage function.
33739203	4	85	theme	2.5/4.0	1009:1015	arg1	%					1016:1016	%	1016:1016	%	1016:1016	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	8	86	theme	CS/HA/Si-HPMC	2140:2152	arg1	hydrogels					2189:2197	CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels	2140:2197	CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels	2140:2197	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	8	87	theme	developed.The	1890:1902	arg1	hydrogel					1918:1925	developed.The CS/HA/Si-HPMC hydrogel	1890:1925	developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering	1890:2252	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	4	88	theme	%	989:989	arg1	Si-HPMC					997:1003	3.0% (w/v) Si-HPMC	986:1003	3.0% (w/v) Si-HPMC	986:1003	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	5	89	dep	In	1174:1175	arg1	vitro					1177:1181	vitro	1177:1181	vitro	1177:1181	In vitro studies of CS/HA and CS/HA/Si-HPMC hydrogels encapsulated in chondrocytes have shown that the proper amount of Si-HPMC increases the proliferation and deposition of the cartilage extracellular matrix.
33739203	8	90	theme	approach.RESEARCH	1801:1817	arg1	hydrogel					1843:1850	a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel	1781:1850	a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering	1781:2252	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	5	91	theme	cartilage	1352:1360	arg1	matrix					1376:1381	the cartilage extracellular matrix	1348:1381	the cartilage extracellular matrix	1348:1381	In vitro studies of CS/HA and CS/HA/Si-HPMC hydrogels encapsulated in chondrocytes have shown that the proper amount of Si-HPMC increases the proliferation and deposition of the cartilage extracellular matrix.
33739203	0	92	theme	network	158:164	arg1	hydrogel					166:173	interpenetrating network hydrogel	141:173	interpenetrating network hydrogel for cartilage tissue engineering	141:206	Structural and biological investigation of chitosan/hyaluronic acid with silanized-hydroxypropyl methylcellulose as an injectable reinforced interpenetrating network hydrogel for cartilage tissue engineering.
33739203	6	93	dep	CS/HA/Si-HPMC	1413:1425	arg1	%					1429:1429	3%	1428:1429	3%	1428:1429	The regeneration rate of the CS/HA/Si-HPMC (3%) hydrogel reached about 79.5% at 21 days for long retention periods, indicating relatively good in vivo bone regeneration.
33739203	5	94	theme	matrix	1376:1381	arg1	deposition					1334:1343	deposition	1334:1343	deposition	1334:1343	In vitro studies of CS/HA and CS/HA/Si-HPMC hydrogels encapsulated in chondrocytes have shown that the proper amount of Si-HPMC increases the proliferation and deposition of the cartilage extracellular matrix.
33739203	5	94	theme	matrix	1376:1381	arg1	proliferation					1316:1328	proliferation	1316:1328	proliferation	1316:1328	In vitro studies of CS/HA and CS/HA/Si-HPMC hydrogels encapsulated in chondrocytes have shown that the proper amount of Si-HPMC increases the proliferation and deposition of the cartilage extracellular matrix.
33739203	6	95	theme	regeneration	1388:1399	arg1	rate					1401:1404	The regeneration rate	1384:1404	The regeneration rate of the CS/HA/Si-HPMC (3%) hydrogel	1384:1439	The regeneration rate of the CS/HA/Si-HPMC (3%) hydrogel reached about 79.5% at 21 days for long retention periods, indicating relatively good in vivo bone regeneration.
33739203	2	96	theme	injectable	558:567	arg1	hydrogels					569:577	injectable hydrogels	558:577	injectable hydrogels for cartilage tissue engineering	558:610	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	2	96	theme	injectable	558:567	arg1	class					362:366	a new class	356:366	a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC)	356:521	In this study, a new class of composite hydrogels derived from water-soluble chitosan (CS)/hyaluronic acid (HA) and silanized-hydroxypropyl methylcellulose (Si-HPMC) (CS/HA/Si-HPMC) has been synthesized and tested as injectable hydrogels for cartilage tissue engineering when combined without the addition of a chemical crosslinking agent.
33739203	8	97	theme	high	2206:2209	arg1	potential					2211:2219	a high potential	2204:2219	a high potential for cartilage tissue engineering	2204:2252	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	1	98	theme	cartilage	321:329	arg1	function					331:338	cartilage function	321:338	cartilage function	321:338	Cartilage damage continues to pose a threat to humans, but no treatment is currently available to fully restore cartilage function.
33739203	8	99	theme	rheological	1948:1958	arg1	concentration					2083:2095	the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration	1936:2095	the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC	1936:2106	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	4	100	theme	suitable	1036:1043	arg1	performance					1054:1064	suitable physical performance	1036:1064	suitable physical performance	1036:1064	Our results demonstrate that the CS and HA-based hydrogel scaffolds, especially the ones with 3.0% (w/v) Si-HPMC and 2.5/4.0% (w/v) CS/HA, have suitable physical performance and bioactive properties, thus provide a potential opportunity to be used for cartilage tissue engineering.
33739203	3	101	theme	Mechanical	681:690	arg1	studies					692:698	Mechanical studies	681:698	Mechanical studies of CS/HA and CS/HA/Si-HPMC hydrogels	681:735	Mechanical studies of CS/HA and CS/HA/Si-HPMC hydrogels showed that as Si-HPMC content increased, swelling rate and rheological properties were higher, compressive strength decreased and degradation was faster.
33739203	8	102	theme	tissue-engineered	1783:1799	arg1	hydrogel					1843:1850	a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel	1781:1850	a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering	1781:2252	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	0	103	theme	chitosan/hyaluronic	43:61	arg1	acid					63:66	chitosan/hyaluronic acid	43:66	chitosan/hyaluronic acid	43:66	Structural and biological investigation of chitosan/hyaluronic acid with silanized-hydroxypropyl methylcellulose as an injectable reinforced interpenetrating network hydrogel for cartilage tissue engineering.
33739203	0	104	with	investigation	26:38	arg1	methylcellulose					97:111	silanized-hydroxypropyl methylcellulose	73:111	silanized-hydroxypropyl methylcellulose as an injectable	73:128	Structural and biological investigation of chitosan/hyaluronic acid with silanized-hydroxypropyl methylcellulose as an injectable reinforced interpenetrating network hydrogel for cartilage tissue engineering.
33739203	8	105	theme	hydrogels.Injectable	2154:2173	arg1	hydrogels					2189:2197	CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels	2140:2197	CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels	2140:2197	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	8	106	theme	tissue	2235:2240	arg1	engineering					2242:2252	cartilage tissue engineering	2225:2252	cartilage tissue engineering	2225:2252	The data provide proof of the principle that the resulting hydrogel has an excellent ability to repair joint cartilage using a tissue-engineered approach.RESEARCH HIGHLIGHTSAn injectable hydrogel based on CS/HA/Si-HPMC composites was developed.The CS/HA/Si-HPMC hydrogel displays the tunable rheological with mechanical properties.The CS/HA/Si-HPMC hydrogel is highly porous with high swelling and degradation ratio.Increasing concentration of Si-HPMC promote an organized network in CS/HA/Si-HPMC hydrogels.Injectable CS/HA/Si-HPMC hydrogels have a high potential for cartilage tissue engineering.
33739203	0	107	theme	cartilage	179:187	arg1	engineering					196:206	cartilage tissue engineering	179:206	cartilage tissue engineering	179:206	Structural and biological investigation of chitosan/hyaluronic acid with silanized-hydroxypropyl methylcellulose as an injectable reinforced interpenetrating network hydrogel for cartilage tissue engineering.
33739203	6	108	theme	retention	1481:1489	arg1	periods					1491:1497	long retention periods	1476:1497	long retention periods	1476:1497	The regeneration rate of the CS/HA/Si-HPMC (3%) hydrogel reached about 79.5% at 21 days for long retention periods, indicating relatively good in vivo bone regeneration.
34414667	8	0	theme	OKGM/PEI	1080:1087	arg1	hydrogels					1103:1111	OKGM/PEI electroactive hydrogels	1080:1111	OKGM/PEI electroactive hydrogels	1080:1111	The rheological behavior and 3D bio-printability of OKGM/PEI electroactive hydrogels were also tested.
34414667	7	1	theme	electroactive	947:959	arg1	hydrogels					971:979	OKGM/PEI electroactive composite hydrogels	938:979	OKGM/PEI electroactive composite hydrogels	938:979	The conductivities of OKGM/PEI electroactive composite hydrogels increased with increasing the content of CNTs.
34414667	10	2	theme	muscle	1307:1312	arg1	regeneration					1339:1350	muscle and cardiac nerve tissue regeneration	1307:1350	muscle and cardiac nerve tissue regeneration	1307:1350	Thus, the OKGM/PEI electroactive hydrogels could be employed as scaffolds for muscle and cardiac nerve tissue regeneration.
34414667	4	3	theme	pH	594:595	arg1	sensitivity					597:607	pH sensitivity	594:607	pH sensitivity	594:607	The OKGM/PEI composite hydrogel displayed self-healing ability and pH sensitivity and showed shear thinning capability, which is suitable for 3D bio-printing technology.
34414667	2	4	theme	solubility	287:296	arg1	properties					267:276	excellent properties	257:276	excellent properties of water solubility, biocompatibility, and biodegradability	257:336	Konjac glucomannan (KGM) exhibits excellent properties of water solubility, biocompatibility, and biodegradability.
34414667	7	5	theme	CNTs	1022:1025	arg1	content					1011:1017	the content	1007:1017	the content of CNTs	1007:1025	The conductivities of OKGM/PEI electroactive composite hydrogels increased with increasing the content of CNTs.
34414667	2	6	theme	water	281:285	arg1	solubility					287:296	water solubility	281:296	water solubility	281:296	Konjac glucomannan (KGM) exhibits excellent properties of water solubility, biocompatibility, and biodegradability.
34414667	0	7	theme	polysaccharide	52:65	arg1	hydrogels					67:75	an electroactive and self-healing polysaccharide hydrogels	18:75	an electroactive and self-healing polysaccharide hydrogels	18:75	3D bioprinting of an electroactive and self-healing polysaccharide hydrogels.
34414667	3	8	theme	amino	478:482	arg1	OKGM					464:467	OKGM	464:467	OKGM	464:467	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	8	theme	amino	478:482	arg1	PEI					521:523	PEI	521:523	PEI	521:523	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	8	theme	amino	478:482	arg1	group					484:488	the amino group	474:488	the amino group of branched polyethyleneimine (PEI)	474:524	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	8	theme	amino	478:482	arg1	polyethyleneimine					502:518	branched polyethyleneimine	493:518	branched polyethyleneimine (PEI)	493:524	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	8	theme	amino	478:482	arg1	glucomannan					451:461	oxidized konjac glucomannan	435:461	oxidized konjac glucomannan (OKGM)	435:468	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	1	9	theme	Polysaccharide	78:91	arg1	alginate					113:120	alginate	113:120	alginate	113:120	Polysaccharide hydrogels including alginate, agarose, hyaluronic acid, and chitosan have been widely used as scaffolds in 3D bio-printing field.
34414667	1	9	theme	Polysaccharide	78:91	arg1	scaffolds					187:195	scaffolds	187:195	scaffolds in 3D bio-printing field	187:220	Polysaccharide hydrogels including alginate, agarose, hyaluronic acid, and chitosan have been widely used as scaffolds in 3D bio-printing field.
34414667	1	9	theme	Polysaccharide	78:91	arg1	agarose					123:129	agarose	123:129	agarose	123:129	Polysaccharide hydrogels including alginate, agarose, hyaluronic acid, and chitosan have been widely used as scaffolds in 3D bio-printing field.
34414667	1	9	theme	Polysaccharide	78:91	arg1	chitosan					153:160	chitosan	153:160	chitosan	153:160	Polysaccharide hydrogels including alginate, agarose, hyaluronic acid, and chitosan have been widely used as scaffolds in 3D bio-printing field.
34414667	1	9	theme	Polysaccharide	78:91	arg1	hydrogels					93:101	Polysaccharide hydrogels	78:101	Polysaccharide hydrogels including alginate, agarose, hyaluronic acid, and chitosan	78:160	Polysaccharide hydrogels including alginate, agarose, hyaluronic acid, and chitosan have been widely used as scaffolds in 3D bio-printing field.
34414667	1	9	theme	Polysaccharide	78:91	arg1	acid					143:146	hyaluronic acid	132:146	hyaluronic acid	132:146	Polysaccharide hydrogels including alginate, agarose, hyaluronic acid, and chitosan have been widely used as scaffolds in 3D bio-printing field.
34414667	10	10	theme	cardiac	1318:1324	arg1	tissue					1332:1337	cardiac nerve tissue	1318:1337	cardiac nerve tissue	1318:1337	Thus, the OKGM/PEI electroactive hydrogels could be employed as scaffolds for muscle and cardiac nerve tissue regeneration.
34414667	4	11	theme	bio-printing	672:683	arg1	technology					685:694	3D bio-printing technology	669:694	3D bio-printing technology	669:694	The OKGM/PEI composite hydrogel displayed self-healing ability and pH sensitivity and showed shear thinning capability, which is suitable for 3D bio-printing technology.
34414667	5	12	theme	carbon	779:784	arg1	CNTs					797:800	CNTs	797:800	CNTs	797:800	Furthermore, the OKGM/PEI electroactive composite hydrogel was obtained by adding carbon nanotubes (CNTs).
34414667	5	12	theme	carbon	779:784	arg1	nanotubes					786:794	carbon nanotubes	779:794	carbon nanotubes (CNTs)	779:801	Furthermore, the OKGM/PEI electroactive composite hydrogel was obtained by adding carbon nanotubes (CNTs).
34414667	6	13	theme	rheological	813:823	arg1	behavior					825:832	Then the rheological behavior	804:832	Then the rheological behavior	804:832	Then the rheological behavior and morphology of OKGM/PEI electroactive hydrogels were thoroughly characterized.
34414667	4	14	theme	3D	669:670	arg1	technology					685:694	3D bio-printing technology	669:694	3D bio-printing technology	669:694	The OKGM/PEI composite hydrogel displayed self-healing ability and pH sensitivity and showed shear thinning capability, which is suitable for 3D bio-printing technology.
34414667	10	15	theme	electroactive	1248:1260	arg1	scaffolds					1293:1301	scaffolds	1293:1301	scaffolds for muscle and cardiac nerve tissue regeneration	1293:1350	Thus, the OKGM/PEI electroactive hydrogels could be employed as scaffolds for muscle and cardiac nerve tissue regeneration.
34414667	10	15	theme	electroactive	1248:1260	arg1	hydrogels					1262:1270	the OKGM/PEI electroactive hydrogels	1235:1270	the OKGM/PEI electroactive hydrogels	1235:1270	Thus, the OKGM/PEI electroactive hydrogels could be employed as scaffolds for muscle and cardiac nerve tissue regeneration.
34414667	3	16	theme	konjac	444:449	arg1	OKGM					464:467	OKGM	464:467	OKGM	464:467	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	16	theme	konjac	444:449	arg1	glucomannan					451:461	oxidized konjac glucomannan	435:461	oxidized konjac glucomannan (OKGM)	435:468	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	0	17	theme	3D	0:1	arg1	bioprinting					3:13	3D bioprinting	0:13	3D bioprinting of an electroactive and self-healing polysaccharide hydrogels	0:75	3D bioprinting of an electroactive and self-healing polysaccharide hydrogels.
34414667	4	18	theme	self-healing	569:580	arg1	ability					582:588	self-healing ability	569:588	self-healing ability	569:588	The OKGM/PEI composite hydrogel displayed self-healing ability and pH sensitivity and showed shear thinning capability, which is suitable for 3D bio-printing technology.
34414667	8	19	theme	hydrogels	1103:1111	arg1	behavior					1044:1051	rheological behavior	1032:1051	rheological behavior	1032:1051	The rheological behavior and 3D bio-printability of OKGM/PEI electroactive hydrogels were also tested.
34414667	8	19	theme	hydrogels	1103:1111	arg1	bio-printability					1060:1075	3D bio-printability	1057:1075	3D bio-printability	1057:1075	The rheological behavior and 3D bio-printability of OKGM/PEI electroactive hydrogels were also tested.
34414667	3	20	theme	glucomannan	451:461	arg1	PEI					521:523	PEI	521:523	PEI	521:523	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	20	theme	glucomannan	451:461	arg1	glucomannan					451:461	oxidized konjac glucomannan	435:461	oxidized konjac glucomannan (OKGM)	435:468	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	20	theme	glucomannan	451:461	arg1	group					484:488	the amino group	474:488	the amino group of branched polyethyleneimine (PEI)	474:524	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	20	theme	glucomannan	451:461	arg1	OKGM					464:467	OKGM	464:467	OKGM	464:467	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	20	theme	glucomannan	451:461	arg1	group					426:430	the aldehyde group	413:430	the aldehyde group of oxidized konjac glucomannan (OKGM)	413:468	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	20	theme	glucomannan	451:461	arg1	polyethyleneimine					502:518	branched polyethyleneimine	493:518	branched polyethyleneimine (PEI)	493:524	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	21	theme	Schiff-base	384:394	arg1	reaction					396:403	Schiff-base reaction	384:403	Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI)	384:524	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	5	22	theme	electroactive	723:735	arg1	hydrogel					747:754	the OKGM/PEI electroactive composite hydrogel	710:754	the OKGM/PEI electroactive composite hydrogel	710:754	Furthermore, the OKGM/PEI electroactive composite hydrogel was obtained by adding carbon nanotubes (CNTs).
34414667	8	23	theme	electroactive	1089:1101	arg1	hydrogels					1103:1111	OKGM/PEI electroactive hydrogels	1080:1111	OKGM/PEI electroactive hydrogels	1080:1111	The rheological behavior and 3D bio-printability of OKGM/PEI electroactive hydrogels were also tested.
34414667	2	24	theme	biocompatibility	299:314	arg1	properties					267:276	excellent properties	257:276	excellent properties of water solubility, biocompatibility, and biodegradability	257:336	Konjac glucomannan (KGM) exhibits excellent properties of water solubility, biocompatibility, and biodegradability.
34414667	5	25	theme	composite	737:745	arg1	hydrogel					747:754	the OKGM/PEI electroactive composite hydrogel	710:754	the OKGM/PEI electroactive composite hydrogel	710:754	Furthermore, the OKGM/PEI electroactive composite hydrogel was obtained by adding carbon nanotubes (CNTs).
34414667	9	26	theme	OKGM/PEI	1195:1202	arg1	hydrogels					1218:1226	OKGM/PEI electroactive hydrogels	1195:1226	OKGM/PEI electroactive hydrogels	1195:1226	It was found that CNTs can also improve the bio-printability of OKGM/PEI electroactive hydrogels.
34414667	1	27	theme	3D	200:201	arg1	field					216:220	3D bio-printing field	200:220	3D bio-printing field	200:220	Polysaccharide hydrogels including alginate, agarose, hyaluronic acid, and chitosan have been widely used as scaffolds in 3D bio-printing field.
34414667	3	28	theme	polyethyleneimine	502:518	arg1	PEI					521:523	PEI	521:523	PEI	521:523	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	28	theme	polyethyleneimine	502:518	arg1	glucomannan					451:461	oxidized konjac glucomannan	435:461	oxidized konjac glucomannan (OKGM)	435:468	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	28	theme	polyethyleneimine	502:518	arg1	group					484:488	the amino group	474:488	the amino group of branched polyethyleneimine (PEI)	474:524	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	28	theme	polyethyleneimine	502:518	arg1	OKGM					464:467	OKGM	464:467	OKGM	464:467	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	28	theme	polyethyleneimine	502:518	arg1	group					426:430	the aldehyde group	413:430	the aldehyde group of oxidized konjac glucomannan (OKGM)	413:468	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	28	theme	polyethyleneimine	502:518	arg1	polyethyleneimine					502:518	branched polyethyleneimine	493:518	branched polyethyleneimine (PEI)	493:524	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	8	29	dep	behavior	1044:1051	arg1	The					1028:1030	The	1028:1030	The	1028:1030	The rheological behavior and 3D bio-printability of OKGM/PEI electroactive hydrogels were also tested.
34414667	1	30	theme	bio-printing	203:214	arg1	field					216:220	3D bio-printing field	200:220	3D bio-printing field	200:220	Polysaccharide hydrogels including alginate, agarose, hyaluronic acid, and chitosan have been widely used as scaffolds in 3D bio-printing field.
34414667	0	31	theme	electroactive	21:33	arg1	hydrogels					67:75	an electroactive and self-healing polysaccharide hydrogels	18:75	an electroactive and self-healing polysaccharide hydrogels	18:75	3D bioprinting of an electroactive and self-healing polysaccharide hydrogels.
34414667	4	32	theme	OKGM/PEI	531:538	arg1	hydrogel					550:557	The OKGM/PEI composite hydrogel	527:557	The OKGM/PEI composite hydrogel	527:557	The OKGM/PEI composite hydrogel displayed self-healing ability and pH sensitivity and showed shear thinning capability, which is suitable for 3D bio-printing technology.
34414667	3	33	theme	oxidized	435:442	arg1	OKGM					464:467	OKGM	464:467	OKGM	464:467	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	33	theme	oxidized	435:442	arg1	glucomannan					451:461	oxidized konjac glucomannan	435:461	oxidized konjac glucomannan (OKGM)	435:468	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	9	34	theme	electroactive	1204:1216	arg1	hydrogels					1218:1226	OKGM/PEI electroactive hydrogels	1195:1226	OKGM/PEI electroactive hydrogels	1195:1226	It was found that CNTs can also improve the bio-printability of OKGM/PEI electroactive hydrogels.
34414667	3	35	theme	aldehyde	417:424	arg1	OKGM					464:467	OKGM	464:467	OKGM	464:467	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	35	theme	aldehyde	417:424	arg1	group					426:430	the aldehyde group	413:430	the aldehyde group of oxidized konjac glucomannan (OKGM)	413:468	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	35	theme	aldehyde	417:424	arg1	polyethyleneimine					502:518	branched polyethyleneimine	493:518	branched polyethyleneimine (PEI)	493:524	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	35	theme	aldehyde	417:424	arg1	glucomannan					451:461	oxidized konjac glucomannan	435:461	oxidized konjac glucomannan (OKGM)	435:468	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	35	theme	aldehyde	417:424	arg1	PEI					521:523	PEI	521:523	PEI	521:523	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	10	36	theme	OKGM/PEI	1239:1246	arg1	scaffolds					1293:1301	scaffolds	1293:1301	scaffolds for muscle and cardiac nerve tissue regeneration	1293:1350	Thus, the OKGM/PEI electroactive hydrogels could be employed as scaffolds for muscle and cardiac nerve tissue regeneration.
34414667	10	36	theme	OKGM/PEI	1239:1246	arg1	hydrogels					1262:1270	the OKGM/PEI electroactive hydrogels	1235:1270	the OKGM/PEI electroactive hydrogels	1235:1270	Thus, the OKGM/PEI electroactive hydrogels could be employed as scaffolds for muscle and cardiac nerve tissue regeneration.
34414667	3	37	theme	composite	346:354	arg1	hydrogels					356:364	Herein composite hydrogels	339:364	Herein composite hydrogels	339:364	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	2	38	theme	Konjac	223:228	arg1	glucomannan					230:240	Konjac glucomannan	223:240	Konjac glucomannan (KGM)	223:246	Konjac glucomannan (KGM) exhibits excellent properties of water solubility, biocompatibility, and biodegradability.
34414667	2	38	theme	Konjac	223:228	arg1	KGM					243:245	KGM	243:245	KGM	243:245	Konjac glucomannan (KGM) exhibits excellent properties of water solubility, biocompatibility, and biodegradability.
34414667	9	39	theme	hydrogels	1218:1226	arg1	bio-printability					1175:1190	the bio-printability	1171:1190	the bio-printability of OKGM/PEI electroactive hydrogels	1171:1226	It was found that CNTs can also improve the bio-printability of OKGM/PEI electroactive hydrogels.
34414667	3	40	theme	branched	493:500	arg1	PEI					521:523	PEI	521:523	PEI	521:523	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	3	40	theme	branched	493:500	arg1	polyethyleneimine					502:518	branched polyethyleneimine	493:518	branched polyethyleneimine (PEI)	493:524	Herein composite hydrogels were prepared via Schiff-base reaction between the aldehyde group of oxidized konjac glucomannan (OKGM) and the amino group of branched polyethyleneimine (PEI).
34414667	0	41	theme	self-healing	39:50	arg1	hydrogels					67:75	an electroactive and self-healing polysaccharide hydrogels	18:75	an electroactive and self-healing polysaccharide hydrogels	18:75	3D bioprinting of an electroactive and self-healing polysaccharide hydrogels.
34414667	1	42	theme	hyaluronic	132:141	arg1	acid					143:146	hyaluronic acid	132:146	hyaluronic acid	132:146	Polysaccharide hydrogels including alginate, agarose, hyaluronic acid, and chitosan have been widely used as scaffolds in 3D bio-printing field.
34414667	10	43	theme	tissue	1332:1337	arg1	regeneration					1339:1350	muscle and cardiac nerve tissue regeneration	1307:1350	muscle and cardiac nerve tissue regeneration	1307:1350	Thus, the OKGM/PEI electroactive hydrogels could be employed as scaffolds for muscle and cardiac nerve tissue regeneration.
34414667	4	44	theme	composite	540:548	arg1	hydrogel					550:557	The OKGM/PEI composite hydrogel	527:557	The OKGM/PEI composite hydrogel	527:557	The OKGM/PEI composite hydrogel displayed self-healing ability and pH sensitivity and showed shear thinning capability, which is suitable for 3D bio-printing technology.
34414667	6	45	theme	hydrogels	875:883	arg1	morphology					838:847	morphology	838:847	morphology	838:847	Then the rheological behavior and morphology of OKGM/PEI electroactive hydrogels were thoroughly characterized.
34414667	6	45	theme	hydrogels	875:883	arg1	behavior					825:832	Then the rheological behavior	804:832	Then the rheological behavior	804:832	Then the rheological behavior and morphology of OKGM/PEI electroactive hydrogels were thoroughly characterized.
34414667	2	46	theme	excellent	257:265	arg1	properties					267:276	excellent properties	257:276	excellent properties of water solubility, biocompatibility, and biodegradability	257:336	Konjac glucomannan (KGM) exhibits excellent properties of water solubility, biocompatibility, and biodegradability.
34414667	8	47	theme	3D	1057:1058	arg1	bio-printability					1060:1075	3D bio-printability	1057:1075	3D bio-printability	1057:1075	The rheological behavior and 3D bio-printability of OKGM/PEI electroactive hydrogels were also tested.
34414667	10	48	theme	nerve	1326:1330	arg1	tissue					1332:1337	cardiac nerve tissue	1318:1337	cardiac nerve tissue	1318:1337	Thus, the OKGM/PEI electroactive hydrogels could be employed as scaffolds for muscle and cardiac nerve tissue regeneration.
34414667	4	49	theme	thinning	626:633	arg1	capability					635:644	shear thinning capability	620:644	shear thinning capability	620:644	The OKGM/PEI composite hydrogel displayed self-healing ability and pH sensitivity and showed shear thinning capability, which is suitable for 3D bio-printing technology.
34414667	5	50	theme	OKGM/PEI	714:721	arg1	hydrogel					747:754	the OKGM/PEI electroactive composite hydrogel	710:754	the OKGM/PEI electroactive composite hydrogel	710:754	Furthermore, the OKGM/PEI electroactive composite hydrogel was obtained by adding carbon nanotubes (CNTs).
34414667	6	51	theme	electroactive	861:873	arg1	hydrogels					875:883	OKGM/PEI electroactive hydrogels	852:883	OKGM/PEI electroactive hydrogels	852:883	Then the rheological behavior and morphology of OKGM/PEI electroactive hydrogels were thoroughly characterized.
34414667	2	52	theme	biodegradability	321:336	arg1	properties					267:276	excellent properties	257:276	excellent properties of water solubility, biocompatibility, and biodegradability	257:336	Konjac glucomannan (KGM) exhibits excellent properties of water solubility, biocompatibility, and biodegradability.
34414667	7	53	theme	composite	961:969	arg1	hydrogels					971:979	OKGM/PEI electroactive composite hydrogels	938:979	OKGM/PEI electroactive composite hydrogels	938:979	The conductivities of OKGM/PEI electroactive composite hydrogels increased with increasing the content of CNTs.
34414667	4	54	theme	shear	620:624	arg1	capability					635:644	shear thinning capability	620:644	shear thinning capability	620:644	The OKGM/PEI composite hydrogel displayed self-healing ability and pH sensitivity and showed shear thinning capability, which is suitable for 3D bio-printing technology.
34414667	6	55	theme	OKGM/PEI	852:859	arg1	hydrogels					875:883	OKGM/PEI electroactive hydrogels	852:883	OKGM/PEI electroactive hydrogels	852:883	Then the rheological behavior and morphology of OKGM/PEI electroactive hydrogels were thoroughly characterized.
34414667	1	56	used	used	179:182	arg2	hydrogels					93:101	Polysaccharide hydrogels	78:101	Polysaccharide hydrogels including alginate, agarose, hyaluronic acid, and chitosan	78:160	Polysaccharide hydrogels including alginate, agarose, hyaluronic acid, and chitosan have been widely used as scaffolds in 3D bio-printing field.
34414667	1	56	used	used	179:182	arg2	alginate					113:120	alginate	113:120	alginate	113:120	Polysaccharide hydrogels including alginate, agarose, hyaluronic acid, and chitosan have been widely used as scaffolds in 3D bio-printing field.
34414667	1	56	used	used	179:182	arg2	acid					143:146	hyaluronic acid	132:146	hyaluronic acid	132:146	Polysaccharide hydrogels including alginate, agarose, hyaluronic acid, and chitosan have been widely used as scaffolds in 3D bio-printing field.
34414667	1	56	used	used	179:182	arg2	agarose					123:129	agarose	123:129	agarose	123:129	Polysaccharide hydrogels including alginate, agarose, hyaluronic acid, and chitosan have been widely used as scaffolds in 3D bio-printing field.
34414667	1	56	used	used	179:182	arg2	chitosan					153:160	chitosan	153:160	chitosan	153:160	Polysaccharide hydrogels including alginate, agarose, hyaluronic acid, and chitosan have been widely used as scaffolds in 3D bio-printing field.
34414667	1	56	used	used	179:182	arg2	scaffolds					187:195	scaffolds	187:195	scaffolds in 3D bio-printing field	187:220	Polysaccharide hydrogels including alginate, agarose, hyaluronic acid, and chitosan have been widely used as scaffolds in 3D bio-printing field.
34414667	1	57	from	scaffolds	187:195	arg1	field					216:220	3D bio-printing field	200:220	3D bio-printing field	200:220	Polysaccharide hydrogels including alginate, agarose, hyaluronic acid, and chitosan have been widely used as scaffolds in 3D bio-printing field.
34414667	7	58	theme	hydrogels	971:979	arg1	conductivities					920:933	The conductivities	916:933	The conductivities of OKGM/PEI electroactive composite hydrogels	916:979	The conductivities of OKGM/PEI electroactive composite hydrogels increased with increasing the content of CNTs.
34414667	0	59	theme	hydrogels	67:75	arg1	bioprinting					3:13	3D bioprinting	0:13	3D bioprinting of an electroactive and self-healing polysaccharide hydrogels	0:75	3D bioprinting of an electroactive and self-healing polysaccharide hydrogels.
34414667	8	60	theme	rheological	1032:1042	arg1	behavior					1044:1051	rheological behavior	1032:1051	rheological behavior	1032:1051	The rheological behavior and 3D bio-printability of OKGM/PEI electroactive hydrogels were also tested.
34414667	7	61	theme	OKGM/PEI	938:945	arg1	hydrogels					971:979	OKGM/PEI electroactive composite hydrogels	938:979	OKGM/PEI electroactive composite hydrogels	938:979	The conductivities of OKGM/PEI electroactive composite hydrogels increased with increasing the content of CNTs.
34236199	6	0	with	comparison	1464:1473	arg1	explosives					1484:1493	raw explosives	1480:1493	raw explosives	1480:1493	In comparison with raw explosives, the thermal stability and sensitivity performances of the core-shell structure composites were outstanding.
34236199	3	1	theme	energetic	770:778	arg1	CNF					788:790	energetic crystal@CNF	770:790	energetic crystal@CNF	770:790	The core-shell structure and the composition of energetic crystal@CNF were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses.
34236199	2	2	theme	facile	660:665	arg1	method					684:689	a facile water suspension method	658:689	a facile water suspension method	658:689	Biomimetically inspired by the superior properties of CNFs, this paper examined them as the coating material to cover cyclotrimethylenetrinitramine (RDX), cyclotetramethylenetetranitramine (HMX), and hexanitrohexaazaisowurtzitane (CL-20) via a facile water suspension method and the ultrasonic technology.
34236199	3	3	theme	@	787:787	arg1	CNF					788:790	energetic crystal@CNF	770:790	energetic crystal@CNF	770:790	The core-shell structure and the composition of energetic crystal@CNF were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses.
34236199	3	4	theme	X-ray	844:848	arg1	diffraction					850:860	X-ray diffraction	844:860	X-ray diffraction	844:860	The core-shell structure and the composition of energetic crystal@CNF were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses.
34236199	3	5	theme	Raman	904:908	arg1	spectroscopy					910:921	Raman spectroscopy	904:921	Raman spectroscopy	904:921	The core-shell structure and the composition of energetic crystal@CNF were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses.
34236199	6	6	theme	raw	1480:1482	arg1	explosives					1484:1493	raw explosives	1480:1493	raw explosives	1480:1493	In comparison with raw explosives, the thermal stability and sensitivity performances of the core-shell structure composites were outstanding.
34236199	3	7	theme	spectroscopy	910:921	arg1	analyses					961:968	scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses	814:968	scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses	814:968	The core-shell structure and the composition of energetic crystal@CNF were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses.
34236199	1	8	theme	burgeoning	174:183	arg1	category					185:192	a burgeoning category	172:192	a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs)	172:270	Recently, a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs) has captured tremendous attention on account of its entangled nanostructured network, natural abundance, and outstanding mechanical properties.
34236199	3	9	theme	spectroscopy	948:959	arg1	analyses					961:968	scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses	814:968	scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses	814:968	The core-shell structure and the composition of energetic crystal@CNF were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses.
34236199	1	10	theme	tremendous	285:294	arg1	attention					296:304	tremendous attention	285:304	tremendous attention	285:304	Recently, a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs) has captured tremendous attention on account of its entangled nanostructured network, natural abundance, and outstanding mechanical properties.
34236199	7	11	theme	potential	1752:1760	arg1	strategy					1774:1781	a potential fabrication strategy	1750:1781	a potential fabrication strategy for the embellishment of high-explosive crystals	1750:1830	Accordingly, this work demonstrated the rewarding application of coating CNFs uniformly on the surface of energetic crystals, ulteriorly offering a potential fabrication strategy for the embellishment of high-explosive crystals.
34236199	1	12	theme	outstanding	381:391	arg1	properties					404:413	outstanding mechanical properties	381:413	outstanding mechanical properties	381:413	Recently, a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs) has captured tremendous attention on account of its entangled nanostructured network, natural abundance, and outstanding mechanical properties.
34236199	1	13	link	derived	223:229	arg1	CNFs					266:269	CNFs	266:269	CNFs	266:269	Recently, a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs) has captured tremendous attention on account of its entangled nanostructured network, natural abundance, and outstanding mechanical properties.
34236199	1	13	link	derived	223:229	arg1	nanofibers					254:263	biocompatible botanically derived nanomaterial cellulose nanofibers	197:263	biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs)	197:270	Recently, a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs) has captured tremendous attention on account of its entangled nanostructured network, natural abundance, and outstanding mechanical properties.
34236199	4	14	theme	energetic	1158:1166	arg1	crystals					1168:1175	energetic crystals	1158:1175	energetic crystals	1158:1175	The obtained outcomes demonstrated that the dispersibility of the CNF enhanced favorably upon covering the surface of explosive crystals; the interfacial contact ability between CNFs and energetic crystals was also manifested to be increased, which could be ascribed to the interfacial interaction of hydrogen bonds and the electrostatic force of self-assembly.
34236199	4	15	theme	CNF	1037:1039	arg1	dispersibility					1015:1028	the dispersibility	1011:1028	the dispersibility of the CNF	1011:1039	The obtained outcomes demonstrated that the dispersibility of the CNF enhanced favorably upon covering the surface of explosive crystals; the interfacial contact ability between CNFs and energetic crystals was also manifested to be increased, which could be ascribed to the interfacial interaction of hydrogen bonds and the electrostatic force of self-assembly.
34236199	1	16	theme	mechanical	393:402	arg1	properties					404:413	outstanding mechanical properties	381:413	outstanding mechanical properties	381:413	Recently, a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs) has captured tremendous attention on account of its entangled nanostructured network, natural abundance, and outstanding mechanical properties.
34236199	2	17	theme	ultrasonic	699:708	arg1	technology					710:719	the ultrasonic technology	695:719	the ultrasonic technology	695:719	Biomimetically inspired by the superior properties of CNFs, this paper examined them as the coating material to cover cyclotrimethylenetrinitramine (RDX), cyclotetramethylenetetranitramine (HMX), and hexanitrohexaazaisowurtzitane (CL-20) via a facile water suspension method and the ultrasonic technology.
34236199	4	18	theme	explosive	1089:1097	arg1	crystals					1099:1106	explosive crystals	1089:1106	explosive crystals	1089:1106	The obtained outcomes demonstrated that the dispersibility of the CNF enhanced favorably upon covering the surface of explosive crystals; the interfacial contact ability between CNFs and energetic crystals was also manifested to be increased, which could be ascribed to the interfacial interaction of hydrogen bonds and the electrostatic force of self-assembly.
34236199	0	19	theme	Surface	129:135	arg1	Modification					109:120	Modification	109:120	Modification of the Surface of Nitramine Explosives	109:159	Biomimetic-Inspired One-Step Strategy for Improvement of Interfacial Interactions in Cellulose Nanofibers by Modification of the Surface of Nitramine Explosives.
34236199	2	20	theme	CNFs	470:473	arg1	properties					456:465	the superior properties	443:465	the superior properties of CNFs, this paper examined them as the coating material to cover cyclotrimethylenetrinitramine (RDX), cyclotetramethylenetetranitramine (HMX), and hexanitrohexaazaisowurtzitane (CL-20) via a facile water suspension method and the ultrasonic technology	443:719	Biomimetically inspired by the superior properties of CNFs, this paper examined them as the coating material to cover cyclotrimethylenetrinitramine (RDX), cyclotetramethylenetetranitramine (HMX), and hexanitrohexaazaisowurtzitane (CL-20) via a facile water suspension method and the ultrasonic technology.
34236199	5	21	theme	stable	1350:1355	arg1	construction					1369:1380	the stable crystalloid construction	1346:1380	the stable crystalloid construction of β-HMX and ε-CL-20	1346:1401	In addition, the stable crystalloid construction of β-HMX and ε-CL-20 has been preserved positively in the preparation process.
34236199	2	22	theme	superior	447:454	arg1	properties					456:465	the superior properties	443:465	the superior properties of CNFs, this paper examined them as the coating material to cover cyclotrimethylenetrinitramine (RDX), cyclotetramethylenetetranitramine (HMX), and hexanitrohexaazaisowurtzitane (CL-20) via a facile water suspension method and the ultrasonic technology	443:719	Biomimetically inspired by the superior properties of CNFs, this paper examined them as the coating material to cover cyclotrimethylenetrinitramine (RDX), cyclotetramethylenetetranitramine (HMX), and hexanitrohexaazaisowurtzitane (CL-20) via a facile water suspension method and the ultrasonic technology.
34236199	6	23	theme	thermal	1500:1506	arg1	stability					1508:1516	thermal stability	1500:1516	thermal stability	1500:1516	In comparison with raw explosives, the thermal stability and sensitivity performances of the core-shell structure composites were outstanding.
34236199	3	24	theme	crystal	780:786	arg1	CNF					788:790	energetic crystal@CNF	770:790	energetic crystal@CNF	770:790	The core-shell structure and the composition of energetic crystal@CNF were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses.
34236199	6	25	theme	sensitivity	1522:1532	arg1	performances					1534:1545	the thermal stability and sensitivity performances	1496:1545	the thermal stability and sensitivity performances of the core-shell structure composites	1496:1584	In comparison with raw explosives, the thermal stability and sensitivity performances of the core-shell structure composites were outstanding.
34236199	5	26	theme	preparation	1440:1450	arg1	process					1452:1458	the preparation process	1436:1458	the preparation process	1436:1458	In addition, the stable crystalloid construction of β-HMX and ε-CL-20 has been preserved positively in the preparation process.
34236199	0	27	theme	One-Step	20:27	arg1	Strategy					29:36	One-Step Strategy	20:36	One-Step Strategy for Improvement of Interfacial Interactions in Cellulose Nanofibers	20:104	Biomimetic-Inspired One-Step Strategy for Improvement of Interfacial Interactions in Cellulose Nanofibers by Modification of the Surface of Nitramine Explosives.
34236199	0	28	from	Improvement	42:52	arg1	Nanofibers					95:104	Cellulose Nanofibers	85:104	Cellulose Nanofibers	85:104	Biomimetic-Inspired One-Step Strategy for Improvement of Interfacial Interactions in Cellulose Nanofibers by Modification of the Surface of Nitramine Explosives.
34236199	2	29	theme	suspension	673:682	arg1	method					684:689	a facile water suspension method	658:689	a facile water suspension method	658:689	Biomimetically inspired by the superior properties of CNFs, this paper examined them as the coating material to cover cyclotrimethylenetrinitramine (RDX), cyclotetramethylenetetranitramine (HMX), and hexanitrohexaazaisowurtzitane (CL-20) via a facile water suspension method and the ultrasonic technology.
34236199	4	30	theme	electrostatic	1295:1307	arg1	force					1309:1313	the electrostatic force	1291:1313	the electrostatic force of self-assembly	1291:1330	The obtained outcomes demonstrated that the dispersibility of the CNF enhanced favorably upon covering the surface of explosive crystals; the interfacial contact ability between CNFs and energetic crystals was also manifested to be increased, which could be ascribed to the interfacial interaction of hydrogen bonds and the electrostatic force of self-assembly.
34236199	6	31	theme	composites	1575:1584	arg1	performances					1534:1545	the thermal stability and sensitivity performances	1496:1545	the thermal stability and sensitivity performances of the core-shell structure composites	1496:1584	In comparison with raw explosives, the thermal stability and sensitivity performances of the core-shell structure composites were outstanding.
34236199	1	32	theme	biocompatible	197:209	arg1	CNFs					266:269	CNFs	266:269	CNFs	266:269	Recently, a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs) has captured tremendous attention on account of its entangled nanostructured network, natural abundance, and outstanding mechanical properties.
34236199	1	32	theme	biocompatible	197:209	arg1	nanofibers					254:263	biocompatible botanically derived nanomaterial cellulose nanofibers	197:263	biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs)	197:270	Recently, a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs) has captured tremendous attention on account of its entangled nanostructured network, natural abundance, and outstanding mechanical properties.
34236199	4	33	theme	interfacial	1113:1123	arg1	ability					1133:1139	the interfacial contact ability	1109:1139	the interfacial contact ability between CNFs and energetic crystals	1109:1175	The obtained outcomes demonstrated that the dispersibility of the CNF enhanced favorably upon covering the surface of explosive crystals; the interfacial contact ability between CNFs and energetic crystals was also manifested to be increased, which could be ascribed to the interfacial interaction of hydrogen bonds and the electrostatic force of self-assembly.
34236199	3	34	theme	scanning	814:821	arg1	microscopy					832:841	scanning electron microscopy	814:841	scanning electron microscopy	814:841	The core-shell structure and the composition of energetic crystal@CNF were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses.
34236199	2	35	theme	water	667:671	arg1	method					684:689	a facile water suspension method	658:689	a facile water suspension method	658:689	Biomimetically inspired by the superior properties of CNFs, this paper examined them as the coating material to cover cyclotrimethylenetrinitramine (RDX), cyclotetramethylenetetranitramine (HMX), and hexanitrohexaazaisowurtzitane (CL-20) via a facile water suspension method and the ultrasonic technology.
34236199	4	36	theme	self-assembly	1318:1330	arg1	interaction					1257:1267	the interfacial interaction	1241:1267	the interfacial interaction of hydrogen bonds	1241:1285	The obtained outcomes demonstrated that the dispersibility of the CNF enhanced favorably upon covering the surface of explosive crystals; the interfacial contact ability between CNFs and energetic crystals was also manifested to be increased, which could be ascribed to the interfacial interaction of hydrogen bonds and the electrostatic force of self-assembly.
34236199	4	36	theme	self-assembly	1318:1330	arg1	force					1309:1313	the electrostatic force	1291:1313	the electrostatic force of self-assembly	1291:1330	The obtained outcomes demonstrated that the dispersibility of the CNF enhanced favorably upon covering the surface of explosive crystals; the interfacial contact ability between CNFs and energetic crystals was also manifested to be increased, which could be ascribed to the interfacial interaction of hydrogen bonds and the electrostatic force of self-assembly.
34236199	7	37	theme	high-explosive	1808:1821	arg1	crystals					1823:1830	high-explosive crystals	1808:1830	high-explosive crystals	1808:1830	Accordingly, this work demonstrated the rewarding application of coating CNFs uniformly on the surface of energetic crystals, ulteriorly offering a potential fabrication strategy for the embellishment of high-explosive crystals.
34236199	7	38	theme	CNFs	1677:1680	arg1	application					1654:1664	the rewarding application	1640:1664	the rewarding application of coating CNFs	1640:1680	Accordingly, this work demonstrated the rewarding application of coating CNFs uniformly on the surface of energetic crystals, ulteriorly offering a potential fabrication strategy for the embellishment of high-explosive crystals.
34236199	3	39	theme	electron	823:830	arg1	microscopy					832:841	scanning electron microscopy	814:841	scanning electron microscopy	814:841	The core-shell structure and the composition of energetic crystal@CNF were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses.
34236199	0	40	theme	Nitramine	140:148	arg1	Explosives					150:159	Nitramine Explosives	140:159	Nitramine Explosives	140:159	Biomimetic-Inspired One-Step Strategy for Improvement of Interfacial Interactions in Cellulose Nanofibers by Modification of the Surface of Nitramine Explosives.
34236199	7	41	theme	crystals	1823:1830	arg1	embellishment					1791:1803	the embellishment	1787:1803	the embellishment of high-explosive crystals	1787:1830	Accordingly, this work demonstrated the rewarding application of coating CNFs uniformly on the surface of energetic crystals, ulteriorly offering a potential fabrication strategy for the embellishment of high-explosive crystals.
34236199	1	42	theme	derived	223:229	arg1	CNFs					266:269	CNFs	266:269	CNFs	266:269	Recently, a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs) has captured tremendous attention on account of its entangled nanostructured network, natural abundance, and outstanding mechanical properties.
34236199	1	42	theme	derived	223:229	arg1	nanofibers					254:263	biocompatible botanically derived nanomaterial cellulose nanofibers	197:263	biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs)	197:270	Recently, a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs) has captured tremendous attention on account of its entangled nanostructured network, natural abundance, and outstanding mechanical properties.
34236199	6	43	theme	core-shell	1554:1563	arg1	composites					1575:1584	the core-shell structure composites	1550:1584	the core-shell structure composites	1550:1584	In comparison with raw explosives, the thermal stability and sensitivity performances of the core-shell structure composites were outstanding.
34236199	7	44	theme	fabrication	1762:1772	arg1	strategy					1774:1781	a potential fabrication strategy	1750:1781	a potential fabrication strategy for the embellishment of high-explosive crystals	1750:1830	Accordingly, this work demonstrated the rewarding application of coating CNFs uniformly on the surface of energetic crystals, ulteriorly offering a potential fabrication strategy for the embellishment of high-explosive crystals.
34236199	7	45	theme	coating	1669:1675	arg1	CNFs					1677:1680	coating CNFs	1669:1680	coating CNFs	1669:1680	Accordingly, this work demonstrated the rewarding application of coating CNFs uniformly on the surface of energetic crystals, ulteriorly offering a potential fabrication strategy for the embellishment of high-explosive crystals.
34236199	5	46	theme	crystalloid	1357:1367	arg1	construction					1369:1380	the stable crystalloid construction	1346:1380	the stable crystalloid construction of β-HMX and ε-CL-20	1346:1401	In addition, the stable crystalloid construction of β-HMX and ε-CL-20 has been preserved positively in the preparation process.
34236199	1	47	theme	nanomaterial	231:242	arg1	CNFs					266:269	CNFs	266:269	CNFs	266:269	Recently, a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs) has captured tremendous attention on account of its entangled nanostructured network, natural abundance, and outstanding mechanical properties.
34236199	1	47	theme	nanomaterial	231:242	arg1	nanofibers					254:263	biocompatible botanically derived nanomaterial cellulose nanofibers	197:263	biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs)	197:270	Recently, a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs) has captured tremendous attention on account of its entangled nanostructured network, natural abundance, and outstanding mechanical properties.
34236199	4	48	theme	crystals	1099:1106	arg1	surface					1078:1084	the surface	1074:1084	the surface of explosive crystals	1074:1106	The obtained outcomes demonstrated that the dispersibility of the CNF enhanced favorably upon covering the surface of explosive crystals; the interfacial contact ability between CNFs and energetic crystals was also manifested to be increased, which could be ascribed to the interfacial interaction of hydrogen bonds and the electrostatic force of self-assembly.
34236199	1	49	theme	entangled	324:332	arg1	network					349:355	its entangled nanostructured network	320:355	its entangled nanostructured network	320:355	Recently, a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs) has captured tremendous attention on account of its entangled nanostructured network, natural abundance, and outstanding mechanical properties.
34236199	6	50	theme	structure	1565:1573	arg1	composites					1575:1584	the core-shell structure composites	1550:1584	the core-shell structure composites	1550:1584	In comparison with raw explosives, the thermal stability and sensitivity performances of the core-shell structure composites were outstanding.
34236199	3	51	dep	Fourier	863:869	arg1	transform					871:879	transform	871:879	transform infrared spectroscopy	871:901	The core-shell structure and the composition of energetic crystal@CNF were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses.
34236199	3	52	theme	CNF	788:790	arg1	structure					737:745	The core-shell structure	722:745	The core-shell structure	722:745	The core-shell structure and the composition of energetic crystal@CNF were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses.
34236199	3	52	theme	CNF	788:790	arg1	composition					755:765	the composition	751:765	the composition of energetic crystal@CNF	751:790	The core-shell structure and the composition of energetic crystal@CNF were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses.
34236199	5	53	theme	ε-CL-20	1395:1401	arg1	construction					1369:1380	the stable crystalloid construction	1346:1380	the stable crystalloid construction of β-HMX and ε-CL-20	1346:1401	In addition, the stable crystalloid construction of β-HMX and ε-CL-20 has been preserved positively in the preparation process.
34236199	1	54	theme	cellulose	244:252	arg1	CNFs					266:269	CNFs	266:269	CNFs	266:269	Recently, a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs) has captured tremendous attention on account of its entangled nanostructured network, natural abundance, and outstanding mechanical properties.
34236199	1	54	theme	cellulose	244:252	arg1	nanofibers					254:263	biocompatible botanically derived nanomaterial cellulose nanofibers	197:263	biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs)	197:270	Recently, a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs) has captured tremendous attention on account of its entangled nanostructured network, natural abundance, and outstanding mechanical properties.
34236199	2	55	theme	coating	508:514	arg1	them					496:499	them	496:499	them	496:499	Biomimetically inspired by the superior properties of CNFs, this paper examined them as the coating material to cover cyclotrimethylenetrinitramine (RDX), cyclotetramethylenetetranitramine (HMX), and hexanitrohexaazaisowurtzitane (CL-20) via a facile water suspension method and the ultrasonic technology.
34236199	2	55	theme	coating	508:514	arg1	material					516:523	the coating material	504:523	the coating material	504:523	Biomimetically inspired by the superior properties of CNFs, this paper examined them as the coating material to cover cyclotrimethylenetrinitramine (RDX), cyclotetramethylenetetranitramine (HMX), and hexanitrohexaazaisowurtzitane (CL-20) via a facile water suspension method and the ultrasonic technology.
34236199	1	56	theme	nanostructured	334:347	arg1	network					349:355	its entangled nanostructured network	320:355	its entangled nanostructured network	320:355	Recently, a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs) has captured tremendous attention on account of its entangled nanostructured network, natural abundance, and outstanding mechanical properties.
34236199	0	57	theme	Explosives	150:159	arg1	Surface					129:135	the Surface	125:135	the Surface of Nitramine Explosives	125:159	Biomimetic-Inspired One-Step Strategy for Improvement of Interfacial Interactions in Cellulose Nanofibers by Modification of the Surface of Nitramine Explosives.
34236199	3	58	dep	transform	871:879	arg1	infrared					881:888	infrared	881:888	transform infrared spectroscopy	871:901	The core-shell structure and the composition of energetic crystal@CNF were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses.
34236199	4	59	theme	bonds	1281:1285	arg1	interaction					1257:1267	the interfacial interaction	1241:1267	the interfacial interaction of hydrogen bonds	1241:1285	The obtained outcomes demonstrated that the dispersibility of the CNF enhanced favorably upon covering the surface of explosive crystals; the interfacial contact ability between CNFs and energetic crystals was also manifested to be increased, which could be ascribed to the interfacial interaction of hydrogen bonds and the electrostatic force of self-assembly.
34236199	4	59	theme	bonds	1281:1285	arg1	force					1309:1313	the electrostatic force	1291:1313	the electrostatic force of self-assembly	1291:1330	The obtained outcomes demonstrated that the dispersibility of the CNF enhanced favorably upon covering the surface of explosive crystals; the interfacial contact ability between CNFs and energetic crystals was also manifested to be increased, which could be ascribed to the interfacial interaction of hydrogen bonds and the electrostatic force of self-assembly.
34236199	1	60	theme	nanofibers	254:263	arg1	category					185:192	a burgeoning category	172:192	a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs)	172:270	Recently, a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs) has captured tremendous attention on account of its entangled nanostructured network, natural abundance, and outstanding mechanical properties.
34236199	0	61	theme	Interactions	69:80	arg1	Improvement					42:52	Improvement	42:52	Improvement of Interfacial Interactions in Cellulose Nanofibers	42:104	Biomimetic-Inspired One-Step Strategy for Improvement of Interfacial Interactions in Cellulose Nanofibers by Modification of the Surface of Nitramine Explosives.
34236199	3	62	theme	diffraction	850:860	arg1	analyses					961:968	scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses	814:968	scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses	814:968	The core-shell structure and the composition of energetic crystal@CNF were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses.
34236199	4	63	theme	hydrogen	1272:1279	arg1	bonds					1281:1285	hydrogen bonds	1272:1285	hydrogen bonds	1272:1285	The obtained outcomes demonstrated that the dispersibility of the CNF enhanced favorably upon covering the surface of explosive crystals; the interfacial contact ability between CNFs and energetic crystals was also manifested to be increased, which could be ascribed to the interfacial interaction of hydrogen bonds and the electrostatic force of self-assembly.
34236199	0	64	theme	Interfacial	57:67	arg1	Interactions					69:80	Interfacial Interactions	57:80	Interfacial Interactions	57:80	Biomimetic-Inspired One-Step Strategy for Improvement of Interfacial Interactions in Cellulose Nanofibers by Modification of the Surface of Nitramine Explosives.
34236199	3	65	theme	microscopy	832:841	arg1	analyses					961:968	scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses	814:968	scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses	814:968	The core-shell structure and the composition of energetic crystal@CNF were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses.
34236199	4	66	theme	obtained	975:982	arg1	outcomes					984:991	The obtained outcomes	971:991	The obtained outcomes	971:991	The obtained outcomes demonstrated that the dispersibility of the CNF enhanced favorably upon covering the surface of explosive crystals; the interfacial contact ability between CNFs and energetic crystals was also manifested to be increased, which could be ascribed to the interfacial interaction of hydrogen bonds and the electrostatic force of self-assembly.
34236199	2	67	dep	CNFs	470:473	arg1	examined					487:494	examined	487:494	examined them as the coating material to cover cyclotrimethylenetrinitramine (RDX), cyclotetramethylenetetranitramine (HMX), and hexanitrohexaazaisowurtzitane (CL-20) via a facile water suspension method and the ultrasonic technology	487:719	Biomimetically inspired by the superior properties of CNFs, this paper examined them as the coating material to cover cyclotrimethylenetrinitramine (RDX), cyclotetramethylenetetranitramine (HMX), and hexanitrohexaazaisowurtzitane (CL-20) via a facile water suspension method and the ultrasonic technology.
34236199	3	68	theme	X-ray	928:932	arg1	spectroscopy					948:959	X-ray photoelectron spectroscopy	928:959	X-ray photoelectron spectroscopy	928:959	The core-shell structure and the composition of energetic crystal@CNF were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses.
34236199	1	69	theme	natural	358:364	arg1	abundance					366:374	natural abundance	358:374	natural abundance	358:374	Recently, a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs) has captured tremendous attention on account of its entangled nanostructured network, natural abundance, and outstanding mechanical properties.
34236199	7	70	theme	rewarding	1644:1652	arg1	application					1654:1664	the rewarding application	1640:1664	the rewarding application of coating CNFs	1640:1680	Accordingly, this work demonstrated the rewarding application of coating CNFs uniformly on the surface of energetic crystals, ulteriorly offering a potential fabrication strategy for the embellishment of high-explosive crystals.
34236199	3	71	theme	photoelectron	934:946	arg1	spectroscopy					948:959	X-ray photoelectron spectroscopy	928:959	X-ray photoelectron spectroscopy	928:959	The core-shell structure and the composition of energetic crystal@CNF were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses.
34236199	7	72	theme	crystals	1720:1727	arg1	surface					1699:1705	the surface	1695:1705	the surface of energetic crystals	1695:1727	Accordingly, this work demonstrated the rewarding application of coating CNFs uniformly on the surface of energetic crystals, ulteriorly offering a potential fabrication strategy for the embellishment of high-explosive crystals.
34236199	4	73	theme	interfacial	1245:1255	arg1	interaction					1257:1267	the interfacial interaction	1241:1267	the interfacial interaction of hydrogen bonds	1241:1285	The obtained outcomes demonstrated that the dispersibility of the CNF enhanced favorably upon covering the surface of explosive crystals; the interfacial contact ability between CNFs and energetic crystals was also manifested to be increased, which could be ascribed to the interfacial interaction of hydrogen bonds and the electrostatic force of self-assembly.
34236199	0	74	theme	Cellulose	85:93	arg1	Nanofibers					95:104	Cellulose Nanofibers	85:104	Cellulose Nanofibers	85:104	Biomimetic-Inspired One-Step Strategy for Improvement of Interfacial Interactions in Cellulose Nanofibers by Modification of the Surface of Nitramine Explosives.
34236199	3	75	theme	core-shell	726:735	arg1	structure					737:745	The core-shell structure	722:745	The core-shell structure	722:745	The core-shell structure and the composition of energetic crystal@CNF were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses.
34236199	5	76	theme	β-HMX	1385:1389	arg1	construction					1369:1380	the stable crystalloid construction	1346:1380	the stable crystalloid construction of β-HMX and ε-CL-20	1346:1401	In addition, the stable crystalloid construction of β-HMX and ε-CL-20 has been preserved positively in the preparation process.
34236199	6	77	theme	stability	1508:1516	arg1	performances					1534:1545	the thermal stability and sensitivity performances	1496:1545	the thermal stability and sensitivity performances of the core-shell structure composites	1496:1584	In comparison with raw explosives, the thermal stability and sensitivity performances of the core-shell structure composites were outstanding.
34236199	7	78	theme	energetic	1710:1718	arg1	crystals					1720:1727	energetic crystals	1710:1727	energetic crystals	1710:1727	Accordingly, this work demonstrated the rewarding application of coating CNFs uniformly on the surface of energetic crystals, ulteriorly offering a potential fabrication strategy for the embellishment of high-explosive crystals.
34236199	4	79	theme	contact	1125:1131	arg1	ability					1133:1139	the interfacial contact ability	1109:1139	the interfacial contact ability between CNFs and energetic crystals	1109:1175	The obtained outcomes demonstrated that the dispersibility of the CNF enhanced favorably upon covering the surface of explosive crystals; the interfacial contact ability between CNFs and energetic crystals was also manifested to be increased, which could be ascribed to the interfacial interaction of hydrogen bonds and the electrostatic force of self-assembly.
34236199	3	80	theme	Fourier	863:869	arg1	analyses					961:968	scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses	814:968	scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses	814:968	The core-shell structure and the composition of energetic crystal@CNF were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses.
32522543	7	0	theme	acid	1072:1075	arg1	concentration					1077:1089	the lowest acid concentration	1061:1089	the lowest acid concentration without mechanical treatment (FN0.1)	1061:1126	Whereas, CNFs prepared at the lowest acid concentration without mechanical treatment (FN0.1) led to films with high elongation at break (30.6%) and good tensile strength (12.3 MPa).
32522543	7	1	with	films	1135:1139	arg1	12.3 MPa					1206:1213	12.3 MPa	1206:1213	12.3 MPa	1206:1213	Whereas, CNFs prepared at the lowest acid concentration without mechanical treatment (FN0.1) led to films with high elongation at break (30.6%) and good tensile strength (12.3 MPa).
32522543	7	1	with	films	1135:1139	arg1	break					1165:1169	high elongation at break	1146:1169	high elongation at break (30.6%)	1146:1177	Whereas, CNFs prepared at the lowest acid concentration without mechanical treatment (FN0.1) led to films with high elongation at break (30.6%) and good tensile strength (12.3 MPa).
32522543	7	1	with	films	1135:1139	arg1	%					1176:1176	30.6%	1172:1176	30.6%	1172:1176	Whereas, CNFs prepared at the lowest acid concentration without mechanical treatment (FN0.1) led to films with high elongation at break (30.6%) and good tensile strength (12.3 MPa).
32522543	7	1	with	films	1135:1139	arg1	strength					1196:1203	good tensile strength	1183:1203	good tensile strength (12.3 MPa)	1183:1214	Whereas, CNFs prepared at the lowest acid concentration without mechanical treatment (FN0.1) led to films with high elongation at break (30.6%) and good tensile strength (12.3 MPa).
32522543	6	2	theme	mechanical	923:932	arg1	treatment					934:942	mechanical treatment	923:942	mechanical treatment (FNM1 and FNM10)	923:959	CNFs prepared at higher concentration, followed by mechanical treatment (FNM1 and FNM10), formed films with low moisture (13.66%) and solubility in water (24.1%).
32522543	4	3	theme	Young	795:799	arg1	modulus					803:809	Young's modulus	795:809	Young's modulus	795:809	All aqueous dispersions of CNFs showed gel-like behavior and, when incorporated to the films, CNFs improved their water barrier properties and mechanical resistance as demonstrated by the increase in tensile strength and Young's modulus.
32522543	1	4	with	treatment	274:282	arg1	homogenizer					303:313	high-pressure homogenizer	289:313	high-pressure homogenizer	289:313	Cellulose nanofibers (CNFs) were isolated from unripe banana peel by acid hydrolysis, with different acid concentrations (0.1%, 1.0% and 10% v/v), followed by mechanical treatment with high-pressure homogenizer.
32522543	2	5	theme	starch-based	323:334	arg1	films					336:340	Banana starch-based films	316:340	Banana starch-based films added with CNFs (0.2% w/w) as a reinforcing agent	316:390	Banana starch-based films added with CNFs (0.2% w/w) as a reinforcing agent were produced by the casting method.
32522543	9	6	from	improvements	1415:1426	arg1	optical					1440:1446	optical	1440:1446	optical	1440:1446	The nanocomposite has potential use in food packaging, since the use of CNFs can promote improvements on barrier, optical and mechanical properties.
32522543	9	6	from	improvements	1415:1426	arg1	properties					1463:1472	mechanical properties	1452:1472	mechanical properties	1452:1472	The nanocomposite has potential use in food packaging, since the use of CNFs can promote improvements on barrier, optical and mechanical properties.
32522543	9	6	from	improvements	1415:1426	arg1	barrier					1431:1437	barrier	1431:1437	barrier	1431:1437	The nanocomposite has potential use in food packaging, since the use of CNFs can promote improvements on barrier, optical and mechanical properties.
32522543	1	7	attach	isolated	137:144	arg2	CNFs					126:129	CNFs	126:129	CNFs	126:129	Cellulose nanofibers (CNFs) were isolated from unripe banana peel by acid hydrolysis, with different acid concentrations (0.1%, 1.0% and 10% v/v), followed by mechanical treatment with high-pressure homogenizer.
32522543	1	7	attach	isolated	137:144	arg2	nanofibers					114:123	Cellulose nanofibers	104:123	Cellulose nanofibers (CNFs)	104:130	Cellulose nanofibers (CNFs) were isolated from unripe banana peel by acid hydrolysis, with different acid concentrations (0.1%, 1.0% and 10% v/v), followed by mechanical treatment with high-pressure homogenizer.
32522543	1	7	attach	isolated	137:144	arg1	peel					165:168	unripe banana peel	151:168	unripe banana peel by acid hydrolysis	151:187	Cellulose nanofibers (CNFs) were isolated from unripe banana peel by acid hydrolysis, with different acid concentrations (0.1%, 1.0% and 10% v/v), followed by mechanical treatment with high-pressure homogenizer.
32522543	9	8	theme	mechanical	1452:1461	arg1	properties					1463:1472	mechanical properties	1452:1472	mechanical properties	1452:1472	The nanocomposite has potential use in food packaging, since the use of CNFs can promote improvements on barrier, optical and mechanical properties.
32522543	3	9	theme	dispersions	465:475	arg1	behavior					445:452	The rheological behavior	429:452	The rheological behavior of aqueous dispersions of CNFs (1.0% w/w) and their effects on the properties of nanocomposite films	429:553	The rheological behavior of aqueous dispersions of CNFs (1.0% w/w) and their effects on the properties of nanocomposite films were investigated.
32522543	1	10	dep	isolated	137:144	arg1	followed					251:258	followed	251:258	followed by mechanical treatment with high-pressure homogenizer	251:313	Cellulose nanofibers (CNFs) were isolated from unripe banana peel by acid hydrolysis, with different acid concentrations (0.1%, 1.0% and 10% v/v), followed by mechanical treatment with high-pressure homogenizer.
32522543	10	11	theme	value-added	1618:1628	arg1	material					1630:1637	a value-added material	1616:1637	a value-added material	1616:1637	Cellulose nanofibers isolated from agro-industrial residues offer the potential to reinforce composites of biodegradable polymers, producing a value-added material.
32522543	5	12	theme	composite	854:862	arg1	matrix					864:869	the composite matrix	850:869	the composite matrix	850:869	Moreover, CNFs were well dispersed in the composite matrix.
32522543	3	13	from	dispersions	465:475	arg1	properties					521:530	the properties	517:530	the properties of nanocomposite films	517:553	The rheological behavior of aqueous dispersions of CNFs (1.0% w/w) and their effects on the properties of nanocomposite films were investigated.
32522543	4	14	theme	mechanical	717:726	arg1	resistance					728:737	mechanical resistance	717:737	mechanical resistance	717:737	All aqueous dispersions of CNFs showed gel-like behavior and, when incorporated to the films, CNFs improved their water barrier properties and mechanical resistance as demonstrated by the increase in tensile strength and Young's modulus.
32522543	1	15	theme	%	243:243	arg1	v/v					245:247	0.1%, 1.0% and 10% v/v	226:247	v/v	245:247	Cellulose nanofibers (CNFs) were isolated from unripe banana peel by acid hydrolysis, with different acid concentrations (0.1%, 1.0% and 10% v/v), followed by mechanical treatment with high-pressure homogenizer.
32522543	3	16	theme	rheological	433:443	arg1	behavior					445:452	The rheological behavior	429:452	The rheological behavior of aqueous dispersions of CNFs (1.0% w/w) and their effects on the properties of nanocomposite films	429:553	The rheological behavior of aqueous dispersions of CNFs (1.0% w/w) and their effects on the properties of nanocomposite films were investigated.
32522543	4	17	theme	tensile	774:780	arg1	strength					782:789	tensile strength	774:789	tensile strength	774:789	All aqueous dispersions of CNFs showed gel-like behavior and, when incorporated to the films, CNFs improved their water barrier properties and mechanical resistance as demonstrated by the increase in tensile strength and Young's modulus.
32522543	9	18	from	use	1358:1360	arg1	packaging					1370:1378	food packaging	1365:1378	food packaging	1365:1378	The nanocomposite has potential use in food packaging, since the use of CNFs can promote improvements on barrier, optical and mechanical properties.
32522543	3	19	theme	CNFs	480:483	arg1	dispersions					465:475	aqueous dispersions	457:475	aqueous dispersions of CNFs (1.0% w/w) and their effects on the properties of nanocomposite films	457:553	The rheological behavior of aqueous dispersions of CNFs (1.0% w/w) and their effects on the properties of nanocomposite films were investigated.
32522543	1	20	theme	acid	173:176	arg1	hydrolysis					178:187	acid hydrolysis	173:187	acid hydrolysis	173:187	Cellulose nanofibers (CNFs) were isolated from unripe banana peel by acid hydrolysis, with different acid concentrations (0.1%, 1.0% and 10% v/v), followed by mechanical treatment with high-pressure homogenizer.
32522543	9	21	theme	potential	1348:1356	arg1	use					1358:1360	potential use	1348:1360	potential use in food packaging	1348:1378	The nanocomposite has potential use in food packaging, since the use of CNFs can promote improvements on barrier, optical and mechanical properties.
32522543	4	22	theme	barrier	694:700	arg1	properties					702:711	their water barrier properties	682:711	their water barrier properties	682:711	All aqueous dispersions of CNFs showed gel-like behavior and, when incorporated to the films, CNFs improved their water barrier properties and mechanical resistance as demonstrated by the increase in tensile strength and Young's modulus.
32522543	6	23	theme	low	980:982	arg1	%					999:999	13.66%	994:999	13.66%	994:999	CNFs prepared at higher concentration, followed by mechanical treatment (FNM1 and FNM10), formed films with low moisture (13.66%) and solubility in water (24.1%).
32522543	6	23	theme	low	980:982	arg1	moisture					984:991	low moisture	980:991	low moisture (13.66%)	980:1000	CNFs prepared at higher concentration, followed by mechanical treatment (FNM1 and FNM10), formed films with low moisture (13.66%) and solubility in water (24.1%).
32522543	7	24	theme	high	1146:1149	arg1	break					1165:1169	high elongation at break	1146:1169	high elongation at break (30.6%)	1146:1177	Whereas, CNFs prepared at the lowest acid concentration without mechanical treatment (FN0.1) led to films with high elongation at break (30.6%) and good tensile strength (12.3 MPa).
32522543	7	24	theme	high	1146:1149	arg1	%					1176:1176	30.6%	1172:1176	30.6%	1172:1176	Whereas, CNFs prepared at the lowest acid concentration without mechanical treatment (FN0.1) led to films with high elongation at break (30.6%) and good tensile strength (12.3 MPa).
32522543	1	25	theme	Cellulose	104:112	arg1	nanofibers					114:123	Cellulose nanofibers	104:123	Cellulose nanofibers (CNFs)	104:130	Cellulose nanofibers (CNFs) were isolated from unripe banana peel by acid hydrolysis, with different acid concentrations (0.1%, 1.0% and 10% v/v), followed by mechanical treatment with high-pressure homogenizer.
32522543	1	25	theme	Cellulose	104:112	arg1	CNFs					126:129	CNFs	126:129	CNFs	126:129	Cellulose nanofibers (CNFs) were isolated from unripe banana peel by acid hydrolysis, with different acid concentrations (0.1%, 1.0% and 10% v/v), followed by mechanical treatment with high-pressure homogenizer.
32522543	6	26	dep	treatment	934:942	arg1	FNM10					954:958	FNM10	954:958	FNM10	954:958	CNFs prepared at higher concentration, followed by mechanical treatment (FNM1 and FNM10), formed films with low moisture (13.66%) and solubility in water (24.1%).
32522543	6	26	dep	treatment	934:942	arg1	FNM1					945:948	FNM1	945:948	FNM1	945:948	CNFs prepared at higher concentration, followed by mechanical treatment (FNM1 and FNM10), formed films with low moisture (13.66%) and solubility in water (24.1%).
32522543	0	27	theme	Starch-based	0:11	arg1	nanocomposites					13:26	Starch-based nanocomposites	0:26	Starch-based nanocomposites with cellulose nanofibers	0:52	Starch-based nanocomposites with cellulose nanofibers obtained from chemical and mechanical treatments.
32522543	7	28	theme	tensile	1188:1194	arg1	12.3 MPa					1206:1213	12.3 MPa	1206:1213	12.3 MPa	1206:1213	Whereas, CNFs prepared at the lowest acid concentration without mechanical treatment (FN0.1) led to films with high elongation at break (30.6%) and good tensile strength (12.3 MPa).
32522543	7	28	theme	tensile	1188:1194	arg1	strength					1196:1203	good tensile strength	1183:1203	good tensile strength (12.3 MPa)	1183:1214	Whereas, CNFs prepared at the lowest acid concentration without mechanical treatment (FN0.1) led to films with high elongation at break (30.6%) and good tensile strength (12.3 MPa).
32522543	3	29	from	effects	506:512	arg1	properties					521:530	the properties	517:530	the properties of nanocomposite films	517:553	The rheological behavior of aqueous dispersions of CNFs (1.0% w/w) and their effects on the properties of nanocomposite films were investigated.
32522543	4	30	theme	CNFs	601:604	arg1	dispersions					586:596	All aqueous dispersions	574:596	All aqueous dispersions of CNFs	574:604	All aqueous dispersions of CNFs showed gel-like behavior and, when incorporated to the films, CNFs improved their water barrier properties and mechanical resistance as demonstrated by the increase in tensile strength and Young's modulus.
32522543	0	31	theme	cellulose	33:41	arg1	nanofibers					43:52	cellulose nanofibers	33:52	cellulose nanofibers	33:52	Starch-based nanocomposites with cellulose nanofibers obtained from chemical and mechanical treatments.
32522543	6	32	theme	higher	889:894	arg1	concentration					896:908	higher concentration	889:908	higher concentration	889:908	CNFs prepared at higher concentration, followed by mechanical treatment (FNM1 and FNM10), formed films with low moisture (13.66%) and solubility in water (24.1%).
32522543	9	33	contain	has	1344:1346	arg1	nanocomposite					1330:1342	The nanocomposite	1326:1342	The nanocomposite	1326:1342	The nanocomposite has potential use in food packaging, since the use of CNFs can promote improvements on barrier, optical and mechanical properties.
32522543	9	33	contain	has	1344:1346	arg2	use					1358:1360	potential use	1348:1360	potential use in food packaging	1348:1378	The nanocomposite has potential use in food packaging, since the use of CNFs can promote improvements on barrier, optical and mechanical properties.
32522543	3	34	theme	w/w	491:493	arg1	CNFs					480:483	CNFs	480:483	CNFs (1.0% w/w)	480:494	The rheological behavior of aqueous dispersions of CNFs (1.0% w/w) and their effects on the properties of nanocomposite films were investigated.
32522543	3	34	theme	w/w	491:493	arg1	%					489:489	1.0% w/w	486:493	1.0% w/w	486:493	The rheological behavior of aqueous dispersions of CNFs (1.0% w/w) and their effects on the properties of nanocomposite films were investigated.
32522543	1	35	theme	different	195:203	arg1	concentrations					210:223	different acid concentrations	195:223	different acid concentrations (0.1%, 1.0% and 10% v/v)	195:248	Cellulose nanofibers (CNFs) were isolated from unripe banana peel by acid hydrolysis, with different acid concentrations (0.1%, 1.0% and 10% v/v), followed by mechanical treatment with high-pressure homogenizer.
32522543	2	36	theme	reinforcing	374:384	arg1	agent					386:390	a reinforcing agent	372:390	a reinforcing agent	372:390	Banana starch-based films added with CNFs (0.2% w/w) as a reinforcing agent were produced by the casting method.
32522543	10	37	theme	biodegradable	1582:1594	arg1	polymers					1596:1603	biodegradable polymers	1582:1603	biodegradable polymers	1582:1603	Cellulose nanofibers isolated from agro-industrial residues offer the potential to reinforce composites of biodegradable polymers, producing a value-added material.
32522543	3	38	theme	aqueous	457:463	arg1	dispersions					465:475	aqueous dispersions	457:475	aqueous dispersions of CNFs (1.0% w/w) and their effects on the properties of nanocomposite films	457:553	The rheological behavior of aqueous dispersions of CNFs (1.0% w/w) and their effects on the properties of nanocomposite films were investigated.
32522543	9	39	theme	food	1365:1368	arg1	packaging					1370:1378	food packaging	1365:1378	food packaging	1365:1378	The nanocomposite has potential use in food packaging, since the use of CNFs can promote improvements on barrier, optical and mechanical properties.
32522543	1	40	theme	acid	205:208	arg1	concentrations					210:223	different acid concentrations	195:223	different acid concentrations (0.1%, 1.0% and 10% v/v)	195:248	Cellulose nanofibers (CNFs) were isolated from unripe banana peel by acid hydrolysis, with different acid concentrations (0.1%, 1.0% and 10% v/v), followed by mechanical treatment with high-pressure homogenizer.
32522543	6	41	with	films	969:973	arg1	%					999:999	13.66%	994:999	13.66%	994:999	CNFs prepared at higher concentration, followed by mechanical treatment (FNM1 and FNM10), formed films with low moisture (13.66%) and solubility in water (24.1%).
32522543	6	41	with	films	969:973	arg1	solubility					1006:1015	solubility	1006:1015	solubility	1006:1015	CNFs prepared at higher concentration, followed by mechanical treatment (FNM1 and FNM10), formed films with low moisture (13.66%) and solubility in water (24.1%).
32522543	6	41	with	films	969:973	arg1	moisture					984:991	low moisture	980:991	low moisture (13.66%)	980:1000	CNFs prepared at higher concentration, followed by mechanical treatment (FNM1 and FNM10), formed films with low moisture (13.66%) and solubility in water (24.1%).
32522543	3	42	from	properties	521:530	arg1	dispersions					465:475	aqueous dispersions	457:475	aqueous dispersions of CNFs (1.0% w/w) and their effects on the properties of nanocomposite films	457:553	The rheological behavior of aqueous dispersions of CNFs (1.0% w/w) and their effects on the properties of nanocomposite films were investigated.
32522543	4	43	theme	aqueous	578:584	arg1	dispersions					586:596	All aqueous dispersions	574:596	All aqueous dispersions of CNFs	574:604	All aqueous dispersions of CNFs showed gel-like behavior and, when incorporated to the films, CNFs improved their water barrier properties and mechanical resistance as demonstrated by the increase in tensile strength and Young's modulus.
32522543	2	44	theme	casting	413:419	arg1	method					421:426	the casting method	409:426	the casting method	409:426	Banana starch-based films added with CNFs (0.2% w/w) as a reinforcing agent were produced by the casting method.
32522543	10	45	theme	agro-industrial	1510:1524	arg1	residues					1526:1533	agro-industrial residues	1510:1533	agro-industrial residues	1510:1533	Cellulose nanofibers isolated from agro-industrial residues offer the potential to reinforce composites of biodegradable polymers, producing a value-added material.
32522543	8	46	theme	UV/light	1285:1292	arg1	transmission					1294:1305	lower UV/light transmission	1279:1305	lower UV/light transmission	1279:1305	Regardless of the used CNFs, all the nanocomposites displayed lower UV/light transmission than control film.
32522543	4	47	from	increase	762:769	arg1	strength					782:789	tensile strength	774:789	tensile strength	774:789	All aqueous dispersions of CNFs showed gel-like behavior and, when incorporated to the films, CNFs improved their water barrier properties and mechanical resistance as demonstrated by the increase in tensile strength and Young's modulus.
32522543	2	48	theme	w/w	364:366	arg1	CNFs					353:356	CNFs	353:356	CNFs (0.2% w/w)	353:367	Banana starch-based films added with CNFs (0.2% w/w) as a reinforcing agent were produced by the casting method.
32522543	2	48	theme	w/w	364:366	arg1	%					362:362	0.2% w/w	359:366	0.2% w/w	359:366	Banana starch-based films added with CNFs (0.2% w/w) as a reinforcing agent were produced by the casting method.
32522543	0	49	theme	chemical	68:75	arg1	treatments					92:101	chemical and mechanical treatments	68:101	chemical and mechanical treatments	68:101	Starch-based nanocomposites with cellulose nanofibers obtained from chemical and mechanical treatments.
32522543	1	50	theme	mechanical	263:272	arg1	treatment					274:282	mechanical treatment	263:282	mechanical treatment with high-pressure homogenizer	263:313	Cellulose nanofibers (CNFs) were isolated from unripe banana peel by acid hydrolysis, with different acid concentrations (0.1%, 1.0% and 10% v/v), followed by mechanical treatment with high-pressure homogenizer.
32522543	8	51	theme	lower	1279:1283	arg1	transmission					1294:1305	lower UV/light transmission	1279:1305	lower UV/light transmission	1279:1305	Regardless of the used CNFs, all the nanocomposites displayed lower UV/light transmission than control film.
32522543	3	52	theme	effects	506:512	arg1	dispersions					465:475	aqueous dispersions	457:475	aqueous dispersions of CNFs (1.0% w/w) and their effects on the properties of nanocomposite films	457:553	The rheological behavior of aqueous dispersions of CNFs (1.0% w/w) and their effects on the properties of nanocomposite films were investigated.
32522543	2	53	theme	Banana	316:321	arg1	films					336:340	Banana starch-based films	316:340	Banana starch-based films added with CNFs (0.2% w/w) as a reinforcing agent	316:390	Banana starch-based films added with CNFs (0.2% w/w) as a reinforcing agent were produced by the casting method.
32522543	8	54	theme	used	1235:1238	arg1	CNFs					1240:1243	the used CNFs	1231:1243	the used CNFs	1231:1243	Regardless of the used CNFs, all the nanocomposites displayed lower UV/light transmission than control film.
32522543	0	55	with	nanocomposites	13:26	arg1	nanofibers					43:52	cellulose nanofibers	33:52	cellulose nanofibers	33:52	Starch-based nanocomposites with cellulose nanofibers obtained from chemical and mechanical treatments.
32522543	10	56	attach	isolated	1496:1503	arg2	nanofibers					1485:1494	Cellulose nanofibers	1475:1494	Cellulose nanofibers isolated from agro-industrial residues	1475:1533	Cellulose nanofibers isolated from agro-industrial residues offer the potential to reinforce composites of biodegradable polymers, producing a value-added material.
32522543	10	56	attach	isolated	1496:1503	arg1	residues					1526:1533	agro-industrial residues	1510:1533	agro-industrial residues	1510:1533	Cellulose nanofibers isolated from agro-industrial residues offer the potential to reinforce composites of biodegradable polymers, producing a value-added material.
32522543	7	57	theme	good	1183:1186	arg1	12.3 MPa					1206:1213	12.3 MPa	1206:1213	12.3 MPa	1206:1213	Whereas, CNFs prepared at the lowest acid concentration without mechanical treatment (FN0.1) led to films with high elongation at break (30.6%) and good tensile strength (12.3 MPa).
32522543	7	57	theme	good	1183:1186	arg1	strength					1196:1203	good tensile strength	1183:1203	good tensile strength (12.3 MPa)	1183:1214	Whereas, CNFs prepared at the lowest acid concentration without mechanical treatment (FN0.1) led to films with high elongation at break (30.6%) and good tensile strength (12.3 MPa).
32522543	3	58	theme	films	549:553	arg1	properties					521:530	the properties	517:530	the properties of nanocomposite films	517:553	The rheological behavior of aqueous dispersions of CNFs (1.0% w/w) and their effects on the properties of nanocomposite films were investigated.
32522543	1	59	dep	concentrations	210:223	arg1	v/v					245:247	0.1%, 1.0% and 10% v/v	226:247	v/v	245:247	Cellulose nanofibers (CNFs) were isolated from unripe banana peel by acid hydrolysis, with different acid concentrations (0.1%, 1.0% and 10% v/v), followed by mechanical treatment with high-pressure homogenizer.
32522543	1	59	dep	concentrations	210:223	arg1	%					235:235	0.1%, 1.0% and 10% v/v	226:247	%	235:235	Cellulose nanofibers (CNFs) were isolated from unripe banana peel by acid hydrolysis, with different acid concentrations (0.1%, 1.0% and 10% v/v), followed by mechanical treatment with high-pressure homogenizer.
32522543	7	60	from	break	1165:1169	arg1	at					1162:1163	high elongation at break	1146:1169	high elongation at break (30.6%)	1146:1177	Whereas, CNFs prepared at the lowest acid concentration without mechanical treatment (FN0.1) led to films with high elongation at break (30.6%) and good tensile strength (12.3 MPa).
32522543	7	60	from	break	1165:1169	arg1	elongation					1151:1160	high elongation at break	1146:1169	high elongation at break (30.6%)	1146:1177	Whereas, CNFs prepared at the lowest acid concentration without mechanical treatment (FN0.1) led to films with high elongation at break (30.6%) and good tensile strength (12.3 MPa).
32522543	9	61	theme	CNFs	1398:1401	arg1	use					1391:1393	the use	1387:1393	the use of CNFs	1387:1401	The nanocomposite has potential use in food packaging, since the use of CNFs can promote improvements on barrier, optical and mechanical properties.
32522543	10	62	theme	polymers	1596:1603	arg1	composites					1568:1577	composites	1568:1577	composites of biodegradable polymers	1568:1603	Cellulose nanofibers isolated from agro-industrial residues offer the potential to reinforce composites of biodegradable polymers, producing a value-added material.
32522543	10	62	theme	polymers	1596:1603	arg1	polymers					1596:1603	biodegradable polymers	1582:1603	biodegradable polymers	1582:1603	Cellulose nanofibers isolated from agro-industrial residues offer the potential to reinforce composites of biodegradable polymers, producing a value-added material.
32522543	4	63	theme	water	688:692	arg1	properties					702:711	their water barrier properties	682:711	their water barrier properties	682:711	All aqueous dispersions of CNFs showed gel-like behavior and, when incorporated to the films, CNFs improved their water barrier properties and mechanical resistance as demonstrated by the increase in tensile strength and Young's modulus.
32522543	4	64	theme	gel-like	613:620	arg1	behavior					622:629	gel-like behavior	613:629	gel-like behavior	613:629	All aqueous dispersions of CNFs showed gel-like behavior and, when incorporated to the films, CNFs improved their water barrier properties and mechanical resistance as demonstrated by the increase in tensile strength and Young's modulus.
32522543	1	65	theme	%	229:229	arg1	%					235:235	0.1%, 1.0% and 10% v/v	226:247	%	235:235	Cellulose nanofibers (CNFs) were isolated from unripe banana peel by acid hydrolysis, with different acid concentrations (0.1%, 1.0% and 10% v/v), followed by mechanical treatment with high-pressure homogenizer.
32522543	0	66	theme	mechanical	81:90	arg1	treatments					92:101	chemical and mechanical treatments	68:101	chemical and mechanical treatments	68:101	Starch-based nanocomposites with cellulose nanofibers obtained from chemical and mechanical treatments.
32522543	7	67	theme	elongation	1151:1160	arg1	break					1165:1169	high elongation at break	1146:1169	high elongation at break (30.6%)	1146:1177	Whereas, CNFs prepared at the lowest acid concentration without mechanical treatment (FN0.1) led to films with high elongation at break (30.6%) and good tensile strength (12.3 MPa).
32522543	7	67	theme	elongation	1151:1160	arg1	%					1176:1176	30.6%	1172:1176	30.6%	1172:1176	Whereas, CNFs prepared at the lowest acid concentration without mechanical treatment (FN0.1) led to films with high elongation at break (30.6%) and good tensile strength (12.3 MPa).
32522543	7	68	theme	at	1162:1163	arg1	break					1165:1169	high elongation at break	1146:1169	high elongation at break (30.6%)	1146:1177	Whereas, CNFs prepared at the lowest acid concentration without mechanical treatment (FN0.1) led to films with high elongation at break (30.6%) and good tensile strength (12.3 MPa).
32522543	7	68	theme	at	1162:1163	arg1	%					1176:1176	30.6%	1172:1176	30.6%	1172:1176	Whereas, CNFs prepared at the lowest acid concentration without mechanical treatment (FN0.1) led to films with high elongation at break (30.6%) and good tensile strength (12.3 MPa).
32522543	1	69	theme	unripe	151:156	arg1	peel					165:168	unripe banana peel	151:168	unripe banana peel by acid hydrolysis	151:187	Cellulose nanofibers (CNFs) were isolated from unripe banana peel by acid hydrolysis, with different acid concentrations (0.1%, 1.0% and 10% v/v), followed by mechanical treatment with high-pressure homogenizer.
32522543	10	70	theme	Cellulose	1475:1483	arg1	nanofibers					1485:1494	Cellulose nanofibers	1475:1494	Cellulose nanofibers isolated from agro-industrial residues	1475:1533	Cellulose nanofibers isolated from agro-industrial residues offer the potential to reinforce composites of biodegradable polymers, producing a value-added material.
32522543	7	71	theme	mechanical	1099:1108	arg1	FN0.1					1121:1125	FN0.1	1121:1125	FN0.1	1121:1125	Whereas, CNFs prepared at the lowest acid concentration without mechanical treatment (FN0.1) led to films with high elongation at break (30.6%) and good tensile strength (12.3 MPa).
32522543	7	71	theme	mechanical	1099:1108	arg1	treatment					1110:1118	mechanical treatment	1099:1118	mechanical treatment (FN0.1)	1099:1126	Whereas, CNFs prepared at the lowest acid concentration without mechanical treatment (FN0.1) led to films with high elongation at break (30.6%) and good tensile strength (12.3 MPa).
32522543	1	72	theme	high-pressure	289:301	arg1	homogenizer					303:313	high-pressure homogenizer	289:313	high-pressure homogenizer	289:313	Cellulose nanofibers (CNFs) were isolated from unripe banana peel by acid hydrolysis, with different acid concentrations (0.1%, 1.0% and 10% v/v), followed by mechanical treatment with high-pressure homogenizer.
32522543	3	73	theme	nanocomposite	535:547	arg1	films					549:553	nanocomposite films	535:553	nanocomposite films	535:553	The rheological behavior of aqueous dispersions of CNFs (1.0% w/w) and their effects on the properties of nanocomposite films were investigated.
32522543	1	74	theme	banana	158:163	arg1	peel					165:168	unripe banana peel	151:168	unripe banana peel by acid hydrolysis	151:187	Cellulose nanofibers (CNFs) were isolated from unripe banana peel by acid hydrolysis, with different acid concentrations (0.1%, 1.0% and 10% v/v), followed by mechanical treatment with high-pressure homogenizer.
32522543	3	75	from	CNFs	480:483	arg1	properties					521:530	the properties	517:530	the properties of nanocomposite films	517:553	The rheological behavior of aqueous dispersions of CNFs (1.0% w/w) and their effects on the properties of nanocomposite films were investigated.
32522543	7	76	theme	lowest	1065:1070	arg1	concentration					1077:1089	the lowest acid concentration	1061:1089	the lowest acid concentration without mechanical treatment (FN0.1)	1061:1126	Whereas, CNFs prepared at the lowest acid concentration without mechanical treatment (FN0.1) led to films with high elongation at break (30.6%) and good tensile strength (12.3 MPa).
33989690	4	0	theme	barrier	769:775	arg1	properties					777:786	The contact angle and water barrier properties	741:786	properties	777:786	The contact angle and water barrier properties of the composites were considerably improved by the addition of QAS.
33989690	7	1	theme	A/P/QAS	1168:1174	arg1	composites					1176:1185	A/P/QAS composites	1168:1185	A/P/QAS composites	1168:1185	The Staphylococcus aureus exhibited highest 25 mm for gel and 18.1 mm for film of A/P/QAS composites.
33989690	1	2	theme	biomedical	210:219	arg1	applications					231:242	food and biomedical packaging applications	201:242	food and biomedical packaging applications	201:242	Agar/polyacrylamide/quaternary ammonium silane-based (A/P/QAS-based) composite films were developed for food and biomedical packaging applications.
33989690	7	3	theme	composites	1176:1185	arg1	film					1160:1163	film	1160:1163	film of A/P/QAS composites	1160:1185	The Staphylococcus aureus exhibited highest 25 mm for gel and 18.1 mm for film of A/P/QAS composites.
33989690	8	4	theme	polyacrylamide	1288:1301	arg1	content					1303:1309	polyacrylamide content	1288:1309	polyacrylamide content	1288:1309	All the composites exhibited shear-thinning behavior, and their viscosity increased with increasing polyacrylamide content.
33989690	1	5	theme	packaging	221:229	arg1	applications					231:242	food and biomedical packaging applications	201:242	food and biomedical packaging applications	201:242	Agar/polyacrylamide/quaternary ammonium silane-based (A/P/QAS-based) composite films were developed for food and biomedical packaging applications.
33989690	10	6	theme	A/P/QAS	1520:1526	arg1	properties					1498:1507	The dynamic rheological properties	1474:1507	The dynamic rheological properties of A/P and A/P/QAS composites	1474:1537	The dynamic rheological properties of A/P and A/P/QAS composites indicated that the prepared composites had good mechanical strength.
33989690	8	7	theme	shear-thinning	1217:1230	arg1	behavior					1232:1239	shear-thinning behavior	1217:1239	shear-thinning behavior	1217:1239	All the composites exhibited shear-thinning behavior, and their viscosity increased with increasing polyacrylamide content.
33989690	10	8	theme	A/P	1512:1514	arg1	properties					1498:1507	The dynamic rheological properties	1474:1507	The dynamic rheological properties of A/P and A/P/QAS composites	1474:1537	The dynamic rheological properties of A/P and A/P/QAS composites indicated that the prepared composites had good mechanical strength.
33989690	9	9	theme	prepared	1338:1345	arg1	composites					1356:1365	the prepared hydrogel composites	1334:1365	the prepared hydrogel composites	1334:1365	The storage moduli of the prepared hydrogel composites were in the range of 5000-10,600 Pa at 1 rad/s and increased continuously over the entire frequency range.
33989690	4	10	theme	QAS	852:854	arg1	addition					840:847	the addition	836:847	the addition of QAS	836:854	The contact angle and water barrier properties of the composites were considerably improved by the addition of QAS.
33989690	2	11	theme	various	363:369	arg1	techniques					388:397	various characterization techniques	363:397	various characterization techniques	363:397	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites were characterized by various characterization techniques in terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties.
33989690	5	12	theme	A/P/QAS	886:892	arg1	composite					894:902	the A/P/QAS composite	882:902	the A/P/QAS composite	882:902	To compare WVP values of the A/P/QAS composite with neat AP composite films it reduced nearly 46% (2.45 to 1.32 × 10-9 g/m2 Pas).
33989690	1	13	theme	Agar/polyacrylamide/quaternary	97:126	arg1	films					176:180	Agar/polyacrylamide/quaternary ammonium silane-based (A/P/QAS-based) composite films	97:180	Agar/polyacrylamide/quaternary ammonium silane-based (A/P/QAS-based) composite films	97:180	Agar/polyacrylamide/quaternary ammonium silane-based (A/P/QAS-based) composite films were developed for food and biomedical packaging applications.
33989690	2	14	theme	differential	439:450	arg1	calorimetry					461:471	differential scanning calorimetry	439:471	differential scanning calorimetry analyses	439:480	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites were characterized by various characterization techniques in terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties.
33989690	5	15	theme	g/m2	976:979	arg1	Pas					981:983	2.45 to 1.32 × 10-9 g/m2 Pas	956:983	2.45 to 1.32 × 10-9 g/m2 Pas	956:983	To compare WVP values of the A/P/QAS composite with neat AP composite films it reduced nearly 46% (2.45 to 1.32 × 10-9 g/m2 Pas).
33989690	5	15	theme	g/m2	976:979	arg1	%					953:953	nearly 46%	944:953	nearly 46% (2.45 to 1.32 × 10-9 g/m2 Pas)	944:984	To compare WVP values of the A/P/QAS composite with neat AP composite films it reduced nearly 46% (2.45 to 1.32 × 10-9 g/m2 Pas).
33989690	5	16	theme	composite	917:925	arg1	films					927:931	neat AP composite films	909:931	neat AP composite films	909:931	To compare WVP values of the A/P/QAS composite with neat AP composite films it reduced nearly 46% (2.45 to 1.32 × 10-9 g/m2 Pas).
33989690	4	17	theme	composites	795:804	arg1	properties					777:786	The contact angle and water barrier properties	741:786	properties	777:786	The contact angle and water barrier properties of the composites were considerably improved by the addition of QAS.
33989690	4	17	theme	composites	795:804	arg1	angle					753:757	The contact angle and water barrier properties	741:786	angle	753:757	The contact angle and water barrier properties of the composites were considerably improved by the addition of QAS.
33989690	9	18	from	rad/s	1408:1412	arg1	range					1379:1383	the range	1375:1383	the range of 5000-10,600 Pa	1375:1401	The storage moduli of the prepared hydrogel composites were in the range of 5000-10,600 Pa at 1 rad/s and increased continuously over the entire frequency range.
33989690	5	19	theme	AP	914:915	arg1	films					927:931	neat AP composite films	909:931	neat AP composite films	909:931	To compare WVP values of the A/P/QAS composite with neat AP composite films it reduced nearly 46% (2.45 to 1.32 × 10-9 g/m2 Pas).
33989690	1	20	theme	ammonium	128:135	arg1	films					176:180	Agar/polyacrylamide/quaternary ammonium silane-based (A/P/QAS-based) composite films	97:180	Agar/polyacrylamide/quaternary ammonium silane-based (A/P/QAS-based) composite films	97:180	Agar/polyacrylamide/quaternary ammonium silane-based (A/P/QAS-based) composite films were developed for food and biomedical packaging applications.
33989690	2	21	dep	A/P	314:316	arg1	composites					330:339	composites	330:339	composites	330:339	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites were characterized by various characterization techniques in terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties.
33989690	2	21	dep	A/P	314:316	arg1	the					310:312	the	310:312	the	310:312	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites were characterized by various characterization techniques in terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties.
33989690	6	22	theme	antimicrobial	1027:1039	arg1	properties					1041:1050	excellent antimicrobial properties	1017:1050	excellent antimicrobial properties	1017:1050	The A/P/QAS composites showed excellent antimicrobial properties against five different organisms.
33989690	10	23	dep	A/P	1512:1514	arg1	composites					1528:1537	composites	1528:1537	composites	1528:1537	The dynamic rheological properties of A/P and A/P/QAS composites indicated that the prepared composites had good mechanical strength.
33989690	10	24	theme	prepared	1558:1565	arg1	composites					1567:1576	the prepared composites	1554:1576	the prepared composites	1554:1576	The dynamic rheological properties of A/P and A/P/QAS composites indicated that the prepared composites had good mechanical strength.
33989690	1	25	theme	silane-based	137:148	arg1	films					176:180	Agar/polyacrylamide/quaternary ammonium silane-based (A/P/QAS-based) composite films	97:180	Agar/polyacrylamide/quaternary ammonium silane-based (A/P/QAS-based) composite films	97:180	Agar/polyacrylamide/quaternary ammonium silane-based (A/P/QAS-based) composite films were developed for food and biomedical packaging applications.
33989690	2	26	theme	mechanical	483:492	arg1	terms					402:406	terms	402:406	terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties	402:519	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites were characterized by various characterization techniques in terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties.
33989690	9	27	theme	Pa	1400:1401	arg1	range					1379:1383	the range	1375:1383	the range of 5000-10,600 Pa	1375:1401	The storage moduli of the prepared hydrogel composites were in the range of 5000-10,600 Pa at 1 rad/s and increased continuously over the entire frequency range.
33989690	10	28	theme	rheological	1486:1496	arg1	properties					1498:1507	The dynamic rheological properties	1474:1507	The dynamic rheological properties of A/P and A/P/QAS composites	1474:1537	The dynamic rheological properties of A/P and A/P/QAS composites indicated that the prepared composites had good mechanical strength.
33989690	11	29	dep	A/P	1625:1627	arg1	films					1651:1655	composite films	1641:1655	composite films	1641:1655	Biopolymer based A/P and A/P/QAS composite films are suitable for green composite packaging applications.
33989690	0	30	theme	Quaternary	0:9	arg1	ammonium					11:18	Quaternary ammonium	0:18	Quaternary ammonium	0:18	Quaternary ammonium silane-reinforced agar/polyacrylamide composites for packaging applications.
33989690	6	31	theme	excellent	1017:1025	arg1	properties					1041:1050	excellent antimicrobial properties	1017:1050	excellent antimicrobial properties	1017:1050	The A/P/QAS composites showed excellent antimicrobial properties against five different organisms.
33989690	9	32	theme	hydrogel	1347:1354	arg1	composites					1356:1365	the prepared hydrogel composites	1334:1365	the prepared hydrogel composites	1334:1365	The storage moduli of the prepared hydrogel composites were in the range of 5000-10,600 Pa at 1 rad/s and increased continuously over the entire frequency range.
33989690	10	33	theme	dynamic	1478:1484	arg1	properties					1498:1507	The dynamic rheological properties	1474:1507	The dynamic rheological properties of A/P and A/P/QAS composites	1474:1537	The dynamic rheological properties of A/P and A/P/QAS composites indicated that the prepared composites had good mechanical strength.
33989690	1	34	theme	A/P/QAS-based	151:163	arg1	films					176:180	Agar/polyacrylamide/quaternary ammonium silane-based (A/P/QAS-based) composite films	97:180	Agar/polyacrylamide/quaternary ammonium silane-based (A/P/QAS-based) composite films	97:180	Agar/polyacrylamide/quaternary ammonium silane-based (A/P/QAS-based) composite films were developed for food and biomedical packaging applications.
33989690	2	35	theme	analyses	473:480	arg1	terms					402:406	terms	402:406	terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties	402:519	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites were characterized by various characterization techniques in terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties.
33989690	0	36	theme	agar/polyacrylamide	38:56	arg1	composites					58:67	agar/polyacrylamide composites	38:67	agar/polyacrylamide composites for packaging applications	38:94	Quaternary ammonium silane-reinforced agar/polyacrylamide composites for packaging applications.
33989690	3	37	theme	prepared	671:678	arg1	composites					680:689	the prepared composites	667:689	the prepared composites	667:689	Results showed that the 5% gravimetric loss (57.8-139.1 °C), glass transition temperature (179-189.9 °C) and tensile strength (35.2-47.8 MPa) of the prepared composites increased with increasing polyacrylamide content.
33989690	10	38	theme	good	1582:1585	arg1	strength					1598:1605	good mechanical strength	1582:1605	good mechanical strength	1582:1605	The dynamic rheological properties of A/P and A/P/QAS composites indicated that the prepared composites had good mechanical strength.
33989690	2	39	theme	characterization	371:386	arg1	techniques					388:397	various characterization techniques	363:397	various characterization techniques	363:397	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites were characterized by various characterization techniques in terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties.
33989690	11	40	theme	composite	1641:1649	arg1	films					1651:1655	composite films	1641:1655	composite films	1641:1655	Biopolymer based A/P and A/P/QAS composite films are suitable for green composite packaging applications.
33989690	5	41	theme	neat	909:912	arg1	films					927:931	neat AP composite films	909:931	neat AP composite films	909:931	To compare WVP values of the A/P/QAS composite with neat AP composite films it reduced nearly 46% (2.45 to 1.32 × 10-9 g/m2 Pas).
33989690	2	42	theme	structural	249:258	arg1	properties					296:305	The structural, optical, and surface morphological properties	245:305	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites	245:339	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites were characterized by various characterization techniques in terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties.
33989690	1	43	theme	composite	166:174	arg1	films					176:180	Agar/polyacrylamide/quaternary ammonium silane-based (A/P/QAS-based) composite films	97:180	Agar/polyacrylamide/quaternary ammonium silane-based (A/P/QAS-based) composite films	97:180	Agar/polyacrylamide/quaternary ammonium silane-based (A/P/QAS-based) composite films were developed for food and biomedical packaging applications.
33989690	1	44	theme	food	201:204	arg1	applications					231:242	food and biomedical packaging applications	201:242	food and biomedical packaging applications	201:242	Agar/polyacrylamide/quaternary ammonium silane-based (A/P/QAS-based) composite films were developed for food and biomedical packaging applications.
33989690	3	45	theme	glass	583:587	arg1	transition					589:598	glass transition	583:598	glass transition temperature (179-189.9 °C)	583:625	Results showed that the 5% gravimetric loss (57.8-139.1 °C), glass transition temperature (179-189.9 °C) and tensile strength (35.2-47.8 MPa) of the prepared composites increased with increasing polyacrylamide content.
33989690	6	46	theme	A/P/QAS	991:997	arg1	composites					999:1008	The A/P/QAS composites	987:1008	The A/P/QAS composites	987:1008	The A/P/QAS composites showed excellent antimicrobial properties against five different organisms.
33989690	3	47	theme	%	547:547	arg1	loss					561:564	the 5% gravimetric loss	542:564	the 5% gravimetric loss (57.8-139.1 °C)	542:580	Results showed that the 5% gravimetric loss (57.8-139.1 °C), glass transition temperature (179-189.9 °C) and tensile strength (35.2-47.8 MPa) of the prepared composites increased with increasing polyacrylamide content.
33989690	3	47	theme	%	547:547	arg1	°C					578:579	57.8-139.1 °C	567:579	57.8-139.1 °C	567:579	Results showed that the 5% gravimetric loss (57.8-139.1 °C), glass transition temperature (179-189.9 °C) and tensile strength (35.2-47.8 MPa) of the prepared composites increased with increasing polyacrylamide content.
33989690	2	48	theme	properties	510:519	arg1	terms					402:406	terms	402:406	terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties	402:519	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites were characterized by various characterization techniques in terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties.
33989690	4	49	theme	contact	745:751	arg1	angle					753:757	The contact angle and water barrier properties	741:786	angle	753:757	The contact angle and water barrier properties of the composites were considerably improved by the addition of QAS.
33989690	5	50	theme	WVP	868:870	arg1	values					872:877	WVP values	868:877	WVP values of the A/P/QAS composite	868:902	To compare WVP values of the A/P/QAS composite with neat AP composite films it reduced nearly 46% (2.45 to 1.32 × 10-9 g/m2 Pas).
33989690	9	51	theme	storage	1316:1322	arg1	moduli					1324:1329	The storage moduli	1312:1329	The storage moduli of the prepared hydrogel composites	1312:1365	The storage moduli of the prepared hydrogel composites were in the range of 5000-10,600 Pa at 1 rad/s and increased continuously over the entire frequency range.
33989690	5	52	theme	composite	894:902	arg1	values					872:877	WVP values	868:877	WVP values of the A/P/QAS composite	868:902	To compare WVP values of the A/P/QAS composite with neat AP composite films it reduced nearly 46% (2.45 to 1.32 × 10-9 g/m2 Pas).
33989690	11	53	theme	composite	1680:1688	arg1	applications					1700:1711	green composite packaging applications	1674:1711	green composite packaging applications	1674:1711	Biopolymer based A/P and A/P/QAS composite films are suitable for green composite packaging applications.
33989690	2	54	theme	rheological	498:508	arg1	properties					510:519	rheological properties	498:519	rheological properties	498:519	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites were characterized by various characterization techniques in terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties.
33989690	3	55	theme	polyacrylamide	717:730	arg1	content					732:738	polyacrylamide content	717:738	polyacrylamide content	717:738	Results showed that the 5% gravimetric loss (57.8-139.1 °C), glass transition temperature (179-189.9 °C) and tensile strength (35.2-47.8 MPa) of the prepared composites increased with increasing polyacrylamide content.
33989690	2	56	theme	morphological	282:294	arg1	properties					296:305	The structural, optical, and surface morphological properties	245:305	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites	245:339	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites were characterized by various characterization techniques in terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties.
33989690	11	57	theme	packaging	1690:1698	arg1	applications					1700:1711	green composite packaging applications	1674:1711	green composite packaging applications	1674:1711	Biopolymer based A/P and A/P/QAS composite films are suitable for green composite packaging applications.
33989690	2	58	theme	analysis	429:436	arg1	terms					402:406	terms	402:406	terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties	402:519	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites were characterized by various characterization techniques in terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties.
33989690	0	59	theme	packaging	73:81	arg1	applications					83:94	packaging applications	73:94	packaging applications	73:94	Quaternary ammonium silane-reinforced agar/polyacrylamide composites for packaging applications.
33989690	7	60	theme	highest	1122:1128	arg1	mm					1133:1134	25 mm	1130:1134	25 mm for gel	1130:1142	The Staphylococcus aureus exhibited highest 25 mm for gel and 18.1 mm for film of A/P/QAS composites.
33989690	5	61	dep	reduced	936:942	arg1	compare					860:866	compare	860:866	To compare WVP values of the A/P/QAS composite with neat AP composite films	857:931	To compare WVP values of the A/P/QAS composite with neat AP composite films it reduced nearly 46% (2.45 to 1.32 × 10-9 g/m2 Pas).
33989690	3	62	theme	transition	589:598	arg1	temperature					600:610	glass transition temperature	583:610	glass transition temperature (179-189.9 °C)	583:625	Results showed that the 5% gravimetric loss (57.8-139.1 °C), glass transition temperature (179-189.9 °C) and tensile strength (35.2-47.8 MPa) of the prepared composites increased with increasing polyacrylamide content.
33989690	3	62	theme	transition	589:598	arg1	°C					623:624	179-189.9 °C	613:624	179-189.9 °C	613:624	Results showed that the 5% gravimetric loss (57.8-139.1 °C), glass transition temperature (179-189.9 °C) and tensile strength (35.2-47.8 MPa) of the prepared composites increased with increasing polyacrylamide content.
33989690	2	63	theme	thermogravimetric	411:427	arg1	analysis					429:436	thermogravimetric analysis	411:436	thermogravimetric analysis	411:436	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites were characterized by various characterization techniques in terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties.
33989690	2	64	theme	A/P/QAS	322:328	arg1	properties					296:305	The structural, optical, and surface morphological properties	245:305	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites	245:339	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites were characterized by various characterization techniques in terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties.
33989690	11	65	theme	green	1674:1678	arg1	applications					1700:1711	green composite packaging applications	1674:1711	green composite packaging applications	1674:1711	Biopolymer based A/P and A/P/QAS composite films are suitable for green composite packaging applications.
33989690	10	66	contain	had	1578:1580	arg2	strength					1598:1605	good mechanical strength	1582:1605	good mechanical strength	1582:1605	The dynamic rheological properties of A/P and A/P/QAS composites indicated that the prepared composites had good mechanical strength.
33989690	10	66	contain	had	1578:1580	arg1	composites					1567:1576	the prepared composites	1554:1576	the prepared composites	1554:1576	The dynamic rheological properties of A/P and A/P/QAS composites indicated that the prepared composites had good mechanical strength.
33989690	3	67	theme	tensile	631:637	arg1	strength					639:646	tensile strength	631:646	tensile strength (35.2-47.8 MPa)	631:662	Results showed that the 5% gravimetric loss (57.8-139.1 °C), glass transition temperature (179-189.9 °C) and tensile strength (35.2-47.8 MPa) of the prepared composites increased with increasing polyacrylamide content.
33989690	3	67	theme	tensile	631:637	arg1	MPa					659:661	35.2-47.8 MPa	649:661	35.2-47.8 MPa	649:661	Results showed that the 5% gravimetric loss (57.8-139.1 °C), glass transition temperature (179-189.9 °C) and tensile strength (35.2-47.8 MPa) of the prepared composites increased with increasing polyacrylamide content.
33989690	9	68	theme	entire	1450:1455	arg1	range					1467:1471	the entire frequency range	1446:1471	the entire frequency range	1446:1471	The storage moduli of the prepared hydrogel composites were in the range of 5000-10,600 Pa at 1 rad/s and increased continuously over the entire frequency range.
33989690	10	69	theme	mechanical	1587:1596	arg1	strength					1598:1605	good mechanical strength	1582:1605	good mechanical strength	1582:1605	The dynamic rheological properties of A/P and A/P/QAS composites indicated that the prepared composites had good mechanical strength.
33989690	2	70	theme	optical	261:267	arg1	properties					296:305	The structural, optical, and surface morphological properties	245:305	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites	245:339	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites were characterized by various characterization techniques in terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties.
33989690	5	71	dep	10-9	971:974	arg1	to					961:962	to	961:962	to	961:962	To compare WVP values of the A/P/QAS composite with neat AP composite films it reduced nearly 46% (2.45 to 1.32 × 10-9 g/m2 Pas).
33989690	4	72	theme	water	763:767	arg1	properties					777:786	The contact angle and water barrier properties	741:786	properties	777:786	The contact angle and water barrier properties of the composites were considerably improved by the addition of QAS.
33989690	9	73	theme	frequency	1457:1465	arg1	range					1467:1471	the entire frequency range	1446:1471	the entire frequency range	1446:1471	The storage moduli of the prepared hydrogel composites were in the range of 5000-10,600 Pa at 1 rad/s and increased continuously over the entire frequency range.
33989690	3	74	theme	gravimetric	549:559	arg1	loss					561:564	the 5% gravimetric loss	542:564	the 5% gravimetric loss (57.8-139.1 °C)	542:580	Results showed that the 5% gravimetric loss (57.8-139.1 °C), glass transition temperature (179-189.9 °C) and tensile strength (35.2-47.8 MPa) of the prepared composites increased with increasing polyacrylamide content.
33989690	3	74	theme	gravimetric	549:559	arg1	°C					578:579	57.8-139.1 °C	567:579	57.8-139.1 °C	567:579	Results showed that the 5% gravimetric loss (57.8-139.1 °C), glass transition temperature (179-189.9 °C) and tensile strength (35.2-47.8 MPa) of the prepared composites increased with increasing polyacrylamide content.
33989690	2	75	theme	A/P	314:316	arg1	properties					296:305	The structural, optical, and surface morphological properties	245:305	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites	245:339	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites were characterized by various characterization techniques in terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties.
33989690	2	76	theme	calorimetry	461:471	arg1	analyses					473:480	differential scanning calorimetry analyses	439:480	differential scanning calorimetry analyses	439:480	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites were characterized by various characterization techniques in terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties.
33989690	3	77	theme	5	546:546	arg1	%					547:547	%	547:547	%	547:547	Results showed that the 5% gravimetric loss (57.8-139.1 °C), glass transition temperature (179-189.9 °C) and tensile strength (35.2-47.8 MPa) of the prepared composites increased with increasing polyacrylamide content.
33989690	9	78	theme	composites	1356:1365	arg1	moduli					1324:1329	The storage moduli	1312:1329	The storage moduli of the prepared hydrogel composites	1312:1365	The storage moduli of the prepared hydrogel composites were in the range of 5000-10,600 Pa at 1 rad/s and increased continuously over the entire frequency range.
33989690	3	79	theme	composites	680:689	arg1	temperature					600:610	glass transition temperature	583:610	glass transition temperature (179-189.9 °C)	583:625	Results showed that the 5% gravimetric loss (57.8-139.1 °C), glass transition temperature (179-189.9 °C) and tensile strength (35.2-47.8 MPa) of the prepared composites increased with increasing polyacrylamide content.
33989690	3	79	theme	composites	680:689	arg1	strength					639:646	tensile strength	631:646	tensile strength (35.2-47.8 MPa)	631:662	Results showed that the 5% gravimetric loss (57.8-139.1 °C), glass transition temperature (179-189.9 °C) and tensile strength (35.2-47.8 MPa) of the prepared composites increased with increasing polyacrylamide content.
33989690	3	79	theme	composites	680:689	arg1	°C					578:579	57.8-139.1 °C	567:579	57.8-139.1 °C	567:579	Results showed that the 5% gravimetric loss (57.8-139.1 °C), glass transition temperature (179-189.9 °C) and tensile strength (35.2-47.8 MPa) of the prepared composites increased with increasing polyacrylamide content.
33989690	3	79	theme	composites	680:689	arg1	°C					623:624	179-189.9 °C	613:624	179-189.9 °C	613:624	Results showed that the 5% gravimetric loss (57.8-139.1 °C), glass transition temperature (179-189.9 °C) and tensile strength (35.2-47.8 MPa) of the prepared composites increased with increasing polyacrylamide content.
33989690	3	79	theme	composites	680:689	arg1	MPa					659:661	35.2-47.8 MPa	649:661	35.2-47.8 MPa	649:661	Results showed that the 5% gravimetric loss (57.8-139.1 °C), glass transition temperature (179-189.9 °C) and tensile strength (35.2-47.8 MPa) of the prepared composites increased with increasing polyacrylamide content.
33989690	3	79	theme	composites	680:689	arg1	loss					561:564	the 5% gravimetric loss	542:564	the 5% gravimetric loss (57.8-139.1 °C)	542:580	Results showed that the 5% gravimetric loss (57.8-139.1 °C), glass transition temperature (179-189.9 °C) and tensile strength (35.2-47.8 MPa) of the prepared composites increased with increasing polyacrylamide content.
33989690	2	80	theme	scanning	452:459	arg1	calorimetry					461:471	differential scanning calorimetry	439:471	differential scanning calorimetry analyses	439:480	The structural, optical, and surface morphological properties of the A/P and A/P/QAS composites were characterized by various characterization techniques in terms of thermogravimetric analysis, differential scanning calorimetry analyses, mechanical and rheological properties.
33989690	6	81	theme	different	1065:1073	arg1	organisms					1075:1083	five different organisms	1060:1083	five different organisms	1060:1083	The A/P/QAS composites showed excellent antimicrobial properties against five different organisms.
32207981	4	0	theme	cell	969:972	arg1	walls					974:978	shoot cell walls	963:978	shoot cell walls	963:978	However, the analysis of sugar constituents of matrix polysaccharides showed that microgravity specifically reduced the amount of glucose (Glc)-containing polysaccharides such as 1,3:1,4-β-glucans, in shoot cell walls.
32207981	3	1	theme	wall	669:672	arg1	wall					669:672	the cell wall	660:672	the cell wall obtained from microgravity-grown shoots	660:712	The bulk amount of the cell wall obtained from microgravity-grown shoots was comparable with that from 1 g-grown shoots.
32207981	3	1	theme	wall	669:672	arg1	comparable					718:727	comparable	718:727	comparable	718:727	The bulk amount of the cell wall obtained from microgravity-grown shoots was comparable with that from 1 g-grown shoots.
32207981	3	1	theme	wall	669:672	arg1	amount					650:655	The bulk amount	641:655	The bulk amount of the cell wall obtained from microgravity-grown shoots	641:712	The bulk amount of the cell wall obtained from microgravity-grown shoots was comparable with that from 1 g-grown shoots.
32207981	2	2	theme	g	575:575	arg1	conditions					594:603	artificial 1 g and microgravity conditions	562:603	artificial 1 g and microgravity conditions	562:603	We investigated the effects of microgravity on the composition of cell wall polysaccharides and on the expression levels of genes involved in cell wall metabolism using rice shoots cultivated under artificial 1 g and microgravity conditions on the International Space Station.
32207981	1	3	with	cell	211:214	arg1	support					232:238	structural support	221:238	structural support	221:238	The plant cell wall provides each cell with structural support and mechanical strength, and thus, it plays an important role in supporting the plant body against the gravitational force.
32207981	1	3	with	cell	211:214	arg1	strength					255:262	mechanical strength	244:262	mechanical strength	244:262	The plant cell wall provides each cell with structural support and mechanical strength, and thus, it plays an important role in supporting the plant body against the gravitational force.
32207981	1	4	theme	plant	320:324	arg1	body					326:329	the plant body	316:329	the plant body against the gravitational force	316:361	The plant cell wall provides each cell with structural support and mechanical strength, and thus, it plays an important role in supporting the plant body against the gravitational force.
32207981	4	5	theme	shoot	963:967	arg1	walls					974:978	shoot cell walls	963:978	shoot cell walls	963:978	However, the analysis of sugar constituents of matrix polysaccharides showed that microgravity specifically reduced the amount of glucose (Glc)-containing polysaccharides such as 1,3:1,4-β-glucans, in shoot cell walls.
32207981	9	6	theme	microgravity	1668:1679	arg1	conditions					1681:1690	microgravity conditions	1668:1690	microgravity conditions in space	1668:1699	Changes in 1,3:1,4-β-glucan level may be involved in the modification of mechanical properties of cell walls under microgravity conditions in space.
32207981	8	7	theme	polysaccharides	1513:1527	arg1	level					1498:1502	the reduced level	1486:1502	the reduced level of these polysaccharides	1486:1527	These results suggest that a microgravity environment promotes the breakdown of 1,3:1,4-β-glucans, which, in turn, causes the reduced level of these polysaccharides in growing rice shoots.
32207981	0	8	theme	Shoots	96:101	arg1	Walls					82:86	Cell Walls	77:86	Cell Walls of Rice Shoots	77:101	Microgravity Affects the Level of Matrix Polysaccharide 1,3:1,4-β-Glucans in Cell Walls of Rice Shoots by Increasing the Expression Level of a Gene Involved in Their Breakdown.
32207981	3	9	with	comparable	718:727	arg1	that					734:737	that	734:737	that	734:737	The bulk amount of the cell wall obtained from microgravity-grown shoots was comparable with that from 1 g-grown shoots.
32207981	2	10	theme	artificial	562:571	arg1	conditions					594:603	artificial 1 g and microgravity conditions	562:603	artificial 1 g and microgravity conditions	562:603	We investigated the effects of microgravity on the composition of cell wall polysaccharides and on the expression levels of genes involved in cell wall metabolism using rice shoots cultivated under artificial 1 g and microgravity conditions on the International Space Station.
32207981	2	11	theme	Space	626:630	arg1	Station					632:638	the International Space Station	608:638	the International Space Station	608:638	We investigated the effects of microgravity on the composition of cell wall polysaccharides and on the expression levels of genes involved in cell wall metabolism using rice shoots cultivated under artificial 1 g and microgravity conditions on the International Space Station.
32207981	2	12	from	effects	384:390	arg1	composition					415:425	the composition	411:425	the composition of cell wall polysaccharides	411:454	We investigated the effects of microgravity on the composition of cell wall polysaccharides and on the expression levels of genes involved in cell wall metabolism using rice shoots cultivated under artificial 1 g and microgravity conditions on the International Space Station.
32207981	2	12	from	effects	384:390	arg1	levels					478:483	the expression levels	463:483	the expression levels of genes involved in cell wall metabolism	463:525	We investigated the effects of microgravity on the composition of cell wall polysaccharides and on the expression levels of genes involved in cell wall metabolism using rice shoots cultivated under artificial 1 g and microgravity conditions on the International Space Station.
32207981	1	13	theme	mechanical	244:253	arg1	strength					255:262	mechanical strength	244:262	mechanical strength	244:262	The plant cell wall provides each cell with structural support and mechanical strength, and thus, it plays an important role in supporting the plant body against the gravitational force.
32207981	0	14	theme	Rice	91:94	arg1	Shoots					96:101	Rice Shoots	91:101	Rice Shoots	91:101	Microgravity Affects the Level of Matrix Polysaccharide 1,3:1,4-β-Glucans in Cell Walls of Rice Shoots by Increasing the Expression Level of a Gene Involved in Their Breakdown.
32207981	0	15	from	1,3:1,4-β-Glucans	56:72	arg1	Walls					82:86	Cell Walls	77:86	Cell Walls of Rice Shoots	77:101	Microgravity Affects the Level of Matrix Polysaccharide 1,3:1,4-β-Glucans in Cell Walls of Rice Shoots by Increasing the Expression Level of a Gene Involved in Their Breakdown.
32207981	2	16	theme	International	612:624	arg1	Space					626:630	International Space	612:630	the International Space Station	608:638	We investigated the effects of microgravity on the composition of cell wall polysaccharides and on the expression levels of genes involved in cell wall metabolism using rice shoots cultivated under artificial 1 g and microgravity conditions on the International Space Station.
32207981	8	17	theme	growing	1532:1538	arg1	shoots					1545:1550	growing rice shoots	1532:1550	growing rice shoots	1532:1550	These results suggest that a microgravity environment promotes the breakdown of 1,3:1,4-β-glucans, which, in turn, causes the reduced level of these polysaccharides in growing rice shoots.
32207981	4	18	theme	matrix	809:814	arg1	polysaccharides					816:830	matrix polysaccharides	809:830	matrix polysaccharides	809:830	However, the analysis of sugar constituents of matrix polysaccharides showed that microgravity specifically reduced the amount of glucose (Glc)-containing polysaccharides such as 1,3:1,4-β-glucans, in shoot cell walls.
32207981	9	19	theme	1,3:1,4-β-glucan	1564:1579	arg1	level					1581:1585	1,3:1,4-β-glucan level	1564:1585	1,3:1,4-β-glucan level	1564:1585	Changes in 1,3:1,4-β-glucan level may be involved in the modification of mechanical properties of cell walls under microgravity conditions in space.
32207981	6	20	theme	gravity	1249:1255	arg1	conditions					1257:1266	both gravity conditions	1244:1266	both gravity conditions	1244:1266	However, the expression levels of genes involved in the biosynthesis of 1,3:1,4-β-glucans were almost the same under both gravity conditions.
32207981	5	21	theme	microgravity	1102:1113	arg1	conditions					1115:1124	microgravity conditions	1102:1124	microgravity conditions	1102:1124	The expression level of a gene for endo-1,3:1,4-β-glucanase, which hydrolyzes 1,3:1,4-β-glucans, largely increased under microgravity conditions.
32207981	2	22	theme	polysaccharides	440:454	arg1	composition					415:425	the composition	411:425	the composition of cell wall polysaccharides	411:454	We investigated the effects of microgravity on the composition of cell wall polysaccharides and on the expression levels of genes involved in cell wall metabolism using rice shoots cultivated under artificial 1 g and microgravity conditions on the International Space Station.
32207981	8	23	theme	rice	1540:1543	arg1	shoots					1545:1550	growing rice shoots	1532:1550	growing rice shoots	1532:1550	These results suggest that a microgravity environment promotes the breakdown of 1,3:1,4-β-glucans, which, in turn, causes the reduced level of these polysaccharides in growing rice shoots.
32207981	2	24	theme	microgravity	581:592	arg1	conditions					594:603	artificial 1 g and microgravity conditions	562:603	artificial 1 g and microgravity conditions	562:603	We investigated the effects of microgravity on the composition of cell wall polysaccharides and on the expression levels of genes involved in cell wall metabolism using rice shoots cultivated under artificial 1 g and microgravity conditions on the International Space Station.
32207981	2	25	theme	expression	467:476	arg1	levels					478:483	the expression levels	463:483	the expression levels of genes involved in cell wall metabolism	463:525	We investigated the effects of microgravity on the composition of cell wall polysaccharides and on the expression levels of genes involved in cell wall metabolism using rice shoots cultivated under artificial 1 g and microgravity conditions on the International Space Station.
32207981	6	26	theme	genes	1161:1165	arg1	same					1233:1236	same	1233:1236	same	1233:1236	However, the expression levels of genes involved in the biosynthesis of 1,3:1,4-β-glucans were almost the same under both gravity conditions.
32207981	6	26	theme	genes	1161:1165	arg1	levels					1151:1156	the expression levels	1136:1156	the expression levels of genes involved in the biosynthesis of 1,3:1,4-β-glucans	1136:1215	However, the expression levels of genes involved in the biosynthesis of 1,3:1,4-β-glucans were almost the same under both gravity conditions.
32207981	4	27	theme	-containing	905:915	arg1	polysaccharides					917:931	glucose (Glc)-containing polysaccharides	892:931	glucose (Glc)-containing polysaccharides such as 1,3:1,4-β-glucans	892:957	However, the analysis of sugar constituents of matrix polysaccharides showed that microgravity specifically reduced the amount of glucose (Glc)-containing polysaccharides such as 1,3:1,4-β-glucans, in shoot cell walls.
32207981	4	27	theme	-containing	905:915	arg1	1,3:1,4-β-glucans					941:957	1,3:1,4-β-glucans	941:957	1,3:1,4-β-glucans	941:957	However, the analysis of sugar constituents of matrix polysaccharides showed that microgravity specifically reduced the amount of glucose (Glc)-containing polysaccharides such as 1,3:1,4-β-glucans, in shoot cell walls.
32207981	4	28	theme	polysaccharides	816:830	arg1	constituents					793:804	sugar constituents	787:804	sugar constituents of matrix polysaccharides	787:830	However, the analysis of sugar constituents of matrix polysaccharides showed that microgravity specifically reduced the amount of glucose (Glc)-containing polysaccharides such as 1,3:1,4-β-glucans, in shoot cell walls.
32207981	7	29	theme	arabinoxylans	1349:1361	arg1	level					1321:1325	the level	1317:1325	the level	1317:1325	On the contrary, microgravity scarcely affected the level and the metabolism of arabinoxylans.
32207981	7	29	theme	arabinoxylans	1349:1361	arg1	metabolism					1335:1344	the metabolism	1331:1344	the metabolism	1331:1344	On the contrary, microgravity scarcely affected the level and the metabolism of arabinoxylans.
32207981	0	30	theme	Expression	121:130	arg1	Level					132:136	the Expression Level	117:136	the Expression Level of a Gene Involved in Their Breakdown	117:174	Microgravity Affects the Level of Matrix Polysaccharide 1,3:1,4-β-Glucans in Cell Walls of Rice Shoots by Increasing the Expression Level of a Gene Involved in Their Breakdown.
32207981	5	31	theme	gene	1007:1010	arg1	level					996:1000	The expression level	981:1000	The expression level of a gene for endo-1,3:1,4-β-glucanase, which hydrolyzes 1,3:1,4-β-glucans,	981:1076	The expression level of a gene for endo-1,3:1,4-β-glucanase, which hydrolyzes 1,3:1,4-β-glucans, largely increased under microgravity conditions.
32207981	4	32	theme	constituents	793:804	arg1	analysis					775:782	the analysis	771:782	the analysis of sugar constituents of matrix polysaccharides	771:830	However, the analysis of sugar constituents of matrix polysaccharides showed that microgravity specifically reduced the amount of glucose (Glc)-containing polysaccharides such as 1,3:1,4-β-glucans, in shoot cell walls.
32207981	1	33	theme	plant	181:185	arg1	wall					192:195	The plant cell wall	177:195	The plant cell wall	177:195	The plant cell wall provides each cell with structural support and mechanical strength, and thus, it plays an important role in supporting the plant body against the gravitational force.
32207981	2	34	theme	microgravity	395:406	arg1	effects					384:390	the effects	380:390	the effects of microgravity on the composition of cell wall polysaccharides and on the expression levels of genes involved in cell wall metabolism	380:525	We investigated the effects of microgravity on the composition of cell wall polysaccharides and on the expression levels of genes involved in cell wall metabolism using rice shoots cultivated under artificial 1 g and microgravity conditions on the International Space Station.
32207981	2	35	theme	wall	511:514	arg1	metabolism					516:525	cell wall metabolism	506:525	cell wall metabolism	506:525	We investigated the effects of microgravity on the composition of cell wall polysaccharides and on the expression levels of genes involved in cell wall metabolism using rice shoots cultivated under artificial 1 g and microgravity conditions on the International Space Station.
32207981	1	36	theme	gravitational	343:355	arg1	force					357:361	the gravitational force	339:361	the gravitational force	339:361	The plant cell wall provides each cell with structural support and mechanical strength, and thus, it plays an important role in supporting the plant body against the gravitational force.
32207981	6	37	theme	expression	1140:1149	arg1	same					1233:1236	same	1233:1236	same	1233:1236	However, the expression levels of genes involved in the biosynthesis of 1,3:1,4-β-glucans were almost the same under both gravity conditions.
32207981	6	37	theme	expression	1140:1149	arg1	levels					1151:1156	the expression levels	1136:1156	the expression levels of genes involved in the biosynthesis of 1,3:1,4-β-glucans	1136:1215	However, the expression levels of genes involved in the biosynthesis of 1,3:1,4-β-glucans were almost the same under both gravity conditions.
32207981	1	38	theme	cell	187:190	arg1	wall					192:195	The plant cell wall	177:195	The plant cell wall	177:195	The plant cell wall provides each cell with structural support and mechanical strength, and thus, it plays an important role in supporting the plant body against the gravitational force.
32207981	0	39	theme	Matrix	34:39	arg1	1,3:1,4-β-Glucans					56:72	Matrix Polysaccharide 1,3:1,4-β-Glucans	34:72	Matrix Polysaccharide 1,3:1,4-β-Glucans in Cell Walls of Rice Shoots	34:101	Microgravity Affects the Level of Matrix Polysaccharide 1,3:1,4-β-Glucans in Cell Walls of Rice Shoots by Increasing the Expression Level of a Gene Involved in Their Breakdown.
32207981	2	40	theme	cell	506:509	arg1	metabolism					516:525	cell wall metabolism	506:525	cell wall metabolism	506:525	We investigated the effects of microgravity on the composition of cell wall polysaccharides and on the expression levels of genes involved in cell wall metabolism using rice shoots cultivated under artificial 1 g and microgravity conditions on the International Space Station.
32207981	0	41	from	Walls	82:86	arg1	Level					25:29	the Level	21:29	the Level of Matrix Polysaccharide 1,3:1,4-β-Glucans in Cell Walls of Rice Shoots	21:101	Microgravity Affects the Level of Matrix Polysaccharide 1,3:1,4-β-Glucans in Cell Walls of Rice Shoots by Increasing the Expression Level of a Gene Involved in Their Breakdown.
32207981	3	42	theme	microgravity-grown	688:705	arg1	shoots					707:712	microgravity-grown shoots	688:712	microgravity-grown shoots	688:712	The bulk amount of the cell wall obtained from microgravity-grown shoots was comparable with that from 1 g-grown shoots.
32207981	2	43	theme	wall	435:438	arg1	polysaccharides					440:454	cell wall polysaccharides	430:454	cell wall polysaccharides	430:454	We investigated the effects of microgravity on the composition of cell wall polysaccharides and on the expression levels of genes involved in cell wall metabolism using rice shoots cultivated under artificial 1 g and microgravity conditions on the International Space Station.
32207981	3	44	theme	g-grown	746:752	arg1	shoots					754:759	1 g-grown shoots	744:759	1 g-grown shoots	744:759	The bulk amount of the cell wall obtained from microgravity-grown shoots was comparable with that from 1 g-grown shoots.
32207981	9	45	theme	cell	1651:1654	arg1	walls					1656:1660	cell walls	1651:1660	cell walls	1651:1660	Changes in 1,3:1,4-β-glucan level may be involved in the modification of mechanical properties of cell walls under microgravity conditions in space.
32207981	0	46	theme	1,3:1,4-β-Glucans	56:72	arg1	Level					25:29	the Level	21:29	the Level of Matrix Polysaccharide 1,3:1,4-β-Glucans in Cell Walls of Rice Shoots	21:101	Microgravity Affects the Level of Matrix Polysaccharide 1,3:1,4-β-Glucans in Cell Walls of Rice Shoots by Increasing the Expression Level of a Gene Involved in Their Breakdown.
32207981	9	47	from	conditions	1681:1690	arg1	space					1695:1699	space	1695:1699	space	1695:1699	Changes in 1,3:1,4-β-glucan level may be involved in the modification of mechanical properties of cell walls under microgravity conditions in space.
32207981	0	48	theme	Gene	143:146	arg1	Level					132:136	the Expression Level	117:136	the Expression Level of a Gene Involved in Their Breakdown	117:174	Microgravity Affects the Level of Matrix Polysaccharide 1,3:1,4-β-Glucans in Cell Walls of Rice Shoots by Increasing the Expression Level of a Gene Involved in Their Breakdown.
32207981	2	49	theme	genes	488:492	arg1	levels					478:483	the expression levels	463:483	the expression levels of genes involved in cell wall metabolism	463:525	We investigated the effects of microgravity on the composition of cell wall polysaccharides and on the expression levels of genes involved in cell wall metabolism using rice shoots cultivated under artificial 1 g and microgravity conditions on the International Space Station.
32207981	4	50	theme	glucose	892:898	arg1	polysaccharides					917:931	glucose (Glc)-containing polysaccharides	892:931	glucose (Glc)-containing polysaccharides such as 1,3:1,4-β-glucans	892:957	However, the analysis of sugar constituents of matrix polysaccharides showed that microgravity specifically reduced the amount of glucose (Glc)-containing polysaccharides such as 1,3:1,4-β-glucans, in shoot cell walls.
32207981	4	50	theme	glucose	892:898	arg1	1,3:1,4-β-glucans					941:957	1,3:1,4-β-glucans	941:957	1,3:1,4-β-glucans	941:957	However, the analysis of sugar constituents of matrix polysaccharides showed that microgravity specifically reduced the amount of glucose (Glc)-containing polysaccharides such as 1,3:1,4-β-glucans, in shoot cell walls.
32207981	0	51	theme	Polysaccharide	41:54	arg1	1,3:1,4-β-Glucans					56:72	Matrix Polysaccharide 1,3:1,4-β-Glucans	34:72	Matrix Polysaccharide 1,3:1,4-β-Glucans in Cell Walls of Rice Shoots	34:101	Microgravity Affects the Level of Matrix Polysaccharide 1,3:1,4-β-Glucans in Cell Walls of Rice Shoots by Increasing the Expression Level of a Gene Involved in Their Breakdown.
32207981	3	52	theme	cell	664:667	arg1	wall					669:672	the cell wall	660:672	the cell wall obtained from microgravity-grown shoots	660:712	The bulk amount of the cell wall obtained from microgravity-grown shoots was comparable with that from 1 g-grown shoots.
32207981	3	53	theme	bulk	645:648	arg1	wall					669:672	the cell wall	660:672	the cell wall obtained from microgravity-grown shoots	660:712	The bulk amount of the cell wall obtained from microgravity-grown shoots was comparable with that from 1 g-grown shoots.
32207981	3	53	theme	bulk	645:648	arg1	comparable					718:727	comparable	718:727	comparable	718:727	The bulk amount of the cell wall obtained from microgravity-grown shoots was comparable with that from 1 g-grown shoots.
32207981	3	53	theme	bulk	645:648	arg1	amount					650:655	The bulk amount	641:655	The bulk amount of the cell wall obtained from microgravity-grown shoots	641:712	The bulk amount of the cell wall obtained from microgravity-grown shoots was comparable with that from 1 g-grown shoots.
32207981	4	54	theme	sugar	787:791	arg1	constituents					793:804	sugar constituents	787:804	sugar constituents of matrix polysaccharides	787:830	However, the analysis of sugar constituents of matrix polysaccharides showed that microgravity specifically reduced the amount of glucose (Glc)-containing polysaccharides such as 1,3:1,4-β-glucans, in shoot cell walls.
32207981	1	55	theme	important	287:295	arg1	role					297:300	an important role	284:300	an important role	284:300	The plant cell wall provides each cell with structural support and mechanical strength, and thus, it plays an important role in supporting the plant body against the gravitational force.
32207981	2	56	theme	rice	533:536	arg1	shoots					538:543	rice shoots	533:543	rice shoots cultivated under artificial 1 g and microgravity conditions on the International Space Station	533:638	We investigated the effects of microgravity on the composition of cell wall polysaccharides and on the expression levels of genes involved in cell wall metabolism using rice shoots cultivated under artificial 1 g and microgravity conditions on the International Space Station.
32207981	9	57	theme	properties	1637:1646	arg1	modification					1610:1621	the modification	1606:1621	the modification of mechanical properties of cell walls	1606:1660	Changes in 1,3:1,4-β-glucan level may be involved in the modification of mechanical properties of cell walls under microgravity conditions in space.
32207981	8	58	theme	1,3:1,4-β-glucans	1444:1460	arg1	breakdown					1431:1439	the breakdown	1427:1439	the breakdown of 1,3:1,4-β-glucans, which, in turn, causes the reduced level of these polysaccharides in growing rice shoots	1427:1550	These results suggest that a microgravity environment promotes the breakdown of 1,3:1,4-β-glucans, which, in turn, causes the reduced level of these polysaccharides in growing rice shoots.
32207981	9	59	theme	walls	1656:1660	arg1	properties					1637:1646	mechanical properties	1626:1646	mechanical properties of cell walls	1626:1660	Changes in 1,3:1,4-β-glucan level may be involved in the modification of mechanical properties of cell walls under microgravity conditions in space.
32207981	8	60	theme	microgravity	1393:1404	arg1	environment					1406:1416	a microgravity environment	1391:1416	a microgravity environment	1391:1416	These results suggest that a microgravity environment promotes the breakdown of 1,3:1,4-β-glucans, which, in turn, causes the reduced level of these polysaccharides in growing rice shoots.
32207981	4	61	theme	polysaccharides	917:931	arg1	polysaccharides					917:931	glucose (Glc)-containing polysaccharides	892:931	glucose (Glc)-containing polysaccharides such as 1,3:1,4-β-glucans	892:957	However, the analysis of sugar constituents of matrix polysaccharides showed that microgravity specifically reduced the amount of glucose (Glc)-containing polysaccharides such as 1,3:1,4-β-glucans, in shoot cell walls.
32207981	4	61	theme	polysaccharides	917:931	arg1	1,3:1,4-β-glucans					941:957	1,3:1,4-β-glucans	941:957	1,3:1,4-β-glucans	941:957	However, the analysis of sugar constituents of matrix polysaccharides showed that microgravity specifically reduced the amount of glucose (Glc)-containing polysaccharides such as 1,3:1,4-β-glucans, in shoot cell walls.
32207981	4	61	theme	polysaccharides	917:931	arg1	amount					882:887	the amount	878:887	the amount of glucose (Glc)-containing polysaccharides such as 1,3:1,4-β-glucans	878:957	However, the analysis of sugar constituents of matrix polysaccharides showed that microgravity specifically reduced the amount of glucose (Glc)-containing polysaccharides such as 1,3:1,4-β-glucans, in shoot cell walls.
32207981	9	62	from	Changes	1553:1559	arg1	level					1581:1585	1,3:1,4-β-glucan level	1564:1585	1,3:1,4-β-glucan level	1564:1585	Changes in 1,3:1,4-β-glucan level may be involved in the modification of mechanical properties of cell walls under microgravity conditions in space.
32207981	0	63	theme	Cell	77:80	arg1	Walls					82:86	Cell Walls	77:86	Cell Walls of Rice Shoots	77:101	Microgravity Affects the Level of Matrix Polysaccharide 1,3:1,4-β-Glucans in Cell Walls of Rice Shoots by Increasing the Expression Level of a Gene Involved in Their Breakdown.
32207981	8	64	theme	reduced	1490:1496	arg1	level					1498:1502	the reduced level	1486:1502	the reduced level of these polysaccharides	1486:1527	These results suggest that a microgravity environment promotes the breakdown of 1,3:1,4-β-glucans, which, in turn, causes the reduced level of these polysaccharides in growing rice shoots.
32207981	6	65	theme	1,3:1,4-β-glucans	1199:1215	arg1	biosynthesis					1183:1194	the biosynthesis	1179:1194	the biosynthesis of 1,3:1,4-β-glucans	1179:1215	However, the expression levels of genes involved in the biosynthesis of 1,3:1,4-β-glucans were almost the same under both gravity conditions.
32207981	2	66	theme	cell	430:433	arg1	polysaccharides					440:454	cell wall polysaccharides	430:454	cell wall polysaccharides	430:454	We investigated the effects of microgravity on the composition of cell wall polysaccharides and on the expression levels of genes involved in cell wall metabolism using rice shoots cultivated under artificial 1 g and microgravity conditions on the International Space Station.
32207981	0	67	from	Level	25:29	arg1	Walls					82:86	Cell Walls	77:86	Cell Walls of Rice Shoots	77:101	Microgravity Affects the Level of Matrix Polysaccharide 1,3:1,4-β-Glucans in Cell Walls of Rice Shoots by Increasing the Expression Level of a Gene Involved in Their Breakdown.
32207981	5	68	theme	expression	985:994	arg1	level					996:1000	The expression level	981:1000	The expression level of a gene for endo-1,3:1,4-β-glucanase, which hydrolyzes 1,3:1,4-β-glucans,	981:1076	The expression level of a gene for endo-1,3:1,4-β-glucanase, which hydrolyzes 1,3:1,4-β-glucans, largely increased under microgravity conditions.
32207981	9	69	theme	mechanical	1626:1635	arg1	properties					1637:1646	mechanical properties	1626:1646	mechanical properties of cell walls	1626:1660	Changes in 1,3:1,4-β-glucan level may be involved in the modification of mechanical properties of cell walls under microgravity conditions in space.
32207981	1	70	theme	structural	221:230	arg1	support					232:238	structural support	221:238	structural support	221:238	The plant cell wall provides each cell with structural support and mechanical strength, and thus, it plays an important role in supporting the plant body against the gravitational force.
34358990	3	0	theme	10	506:507	arg1	%					508:508	%	508:508	%	508:508	The xylitol production in the synthetic, non-detoxified and detoxified hydrolysate media was studied (50 ± 0.5 g/L) using 10% v/v non - induced cells of M. caribbica for 120 h.
34358990	0	1	theme	hemicellulose	64:76	arg1	hydrolysate					78:88	corncob hemicellulose hydrolysate	56:88	corncob hemicellulose hydrolysate for xylose fermentation	56:112	Particle size influence on the composition of sugars in corncob hemicellulose hydrolysate for xylose fermentation by Meyerozyma caribbica.
34358990	0	2	dep	Meyerozyma	117:126	arg1	caribbica					128:136	Meyerozyma caribbica	117:136	Meyerozyma caribbica	117:136	Particle size influence on the composition of sugars in corncob hemicellulose hydrolysate for xylose fermentation by Meyerozyma caribbica.
34358990	0	3	theme	xylose	94:99	arg1	fermentation					101:112	xylose fermentation	94:112	xylose fermentation	94:112	Particle size influence on the composition of sugars in corncob hemicellulose hydrolysate for xylose fermentation by Meyerozyma caribbica.
34358990	5	4	used	used	847:850	arg2	detoxification					723:736	The detoxification	719:736	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm	719:836	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm could be used as the prospective pretreatment process for ecofriendly and industrial scale production of xylitol with M. caribbica.
34358990	5	4	used	used	847:850	arg2	process					884:890	the prospective pretreatment process	855:890	the prospective pretreatment process	855:890	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm could be used as the prospective pretreatment process for ecofriendly and industrial scale production of xylitol with M. caribbica.
34358990	5	5	theme	scale	923:927	arg1	production					929:938	industrial scale production	912:938	industrial scale production of xylitol with M. caribbica	912:967	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm could be used as the prospective pretreatment process for ecofriendly and industrial scale production of xylitol with M. caribbica.
34358990	0	6	from	sugars	46:51	arg1	hydrolysate					78:88	corncob hemicellulose hydrolysate	56:88	corncob hemicellulose hydrolysate for xylose fermentation	56:112	Particle size influence on the composition of sugars in corncob hemicellulose hydrolysate for xylose fermentation by Meyerozyma caribbica.
34358990	2	7	theme	xylose	360:365	arg1	content					349:355	a high content	342:355	a high content of xylose (8.0 ± 0.5 g/L)	342:381	The particle size of 0.02 ± 0.01 to 0.1 ± 0.05 mm was rich in glucose (12.0 ± 0.5 g/L), whereas 0.5 ± 0.25 to 2.0 ± 0.5 mm had a high content of xylose (8.0 ± 0.5 g/L).
34358990	5	8	theme	prospective	859:869	arg1	detoxification					723:736	The detoxification	719:736	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm	719:836	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm could be used as the prospective pretreatment process for ecofriendly and industrial scale production of xylitol with M. caribbica.
34358990	5	8	theme	prospective	859:869	arg1	process					884:890	the prospective pretreatment process	855:890	the prospective pretreatment process	855:890	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm could be used as the prospective pretreatment process for ecofriendly and industrial scale production of xylitol with M. caribbica.
34358990	5	9	theme	particle	794:801	arg1	size					803:806	an identified corncob particle size	772:806	an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm	772:836	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm could be used as the prospective pretreatment process for ecofriendly and industrial scale production of xylitol with M. caribbica.
34358990	4	10	theme	specific	597:604	arg1	rate					613:616	the specific growth rate	593:616	the specific growth rate of 0.056 ± 0.01 (μ)	593:636	At the end of fermentation with the specific growth rate of 0.056 ± 0.01 (μ), xylitol yields of 45.00 ± 1.00%, 10.00 ± 1.00% and 54.00 ± 1.00% were obtained.
34358990	5	11	theme	xylitol	943:949	arg1	ecofriendly					896:906	ecofriendly	896:906	ecofriendly	896:906	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm could be used as the prospective pretreatment process for ecofriendly and industrial scale production of xylitol with M. caribbica.
34358990	5	11	theme	xylitol	943:949	arg1	production					929:938	industrial scale production	912:938	industrial scale production of xylitol with M. caribbica	912:967	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm could be used as the prospective pretreatment process for ecofriendly and industrial scale production of xylitol with M. caribbica.
34358990	5	12	theme	2.0 ± 0.5 mm	825:836	arg1	size					803:806	an identified corncob particle size	772:806	an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm	772:836	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm could be used as the prospective pretreatment process for ecofriendly and industrial scale production of xylitol with M. caribbica.
34358990	0	13	from	composition	31:41	arg1	hydrolysate					78:88	corncob hemicellulose hydrolysate	56:88	corncob hemicellulose hydrolysate for xylose fermentation	56:112	Particle size influence on the composition of sugars in corncob hemicellulose hydrolysate for xylose fermentation by Meyerozyma caribbica.
34358990	3	14	theme	xylitol	388:394	arg1	production					396:405	The xylitol production	384:405	The xylitol production in the synthetic, non-detoxified and detoxified hydrolysate media	384:471	The xylitol production in the synthetic, non-detoxified and detoxified hydrolysate media was studied (50 ± 0.5 g/L) using 10% v/v non - induced cells of M. caribbica for 120 h.
34358990	1	15	theme	acidophilic	181:191	arg1	caribbica					204:212	acidophilic Meyerozyma caribbica	181:212	acidophilic Meyerozyma caribbica	181:212	The xylitol production was performed with acidophilic Meyerozyma caribbica.
34358990	4	16	theme	0.056 ± 0.01	621:632	arg1	rate					613:616	the specific growth rate	593:616	the specific growth rate of 0.056 ± 0.01 (μ)	593:636	At the end of fermentation with the specific growth rate of 0.056 ± 0.01 (μ), xylitol yields of 45.00 ± 1.00%, 10.00 ± 1.00% and 54.00 ± 1.00% were obtained.
34358990	1	17	theme	Meyerozyma	193:202	arg1	caribbica					204:212	acidophilic Meyerozyma caribbica	181:212	acidophilic Meyerozyma caribbica	181:212	The xylitol production was performed with acidophilic Meyerozyma caribbica.
34358990	0	18	theme	Particle	0:7	arg1	size					9:12	Particle size	0:12	Particle size	0:12	Particle size influence on the composition of sugars in corncob hemicellulose hydrolysate for xylose fermentation by Meyerozyma caribbica.
34358990	4	19	theme	%	684:684	arg1	yields					647:652	xylitol yields	639:652	xylitol yields of 45.00 ± 1.00%, 10.00 ± 1.00% and 54.00 ± 1.00%	639:702	At the end of fermentation with the specific growth rate of 0.056 ± 0.01 (μ), xylitol yields of 45.00 ± 1.00%, 10.00 ± 1.00% and 54.00 ± 1.00% were obtained.
34358990	2	20	from	rich	269:272	arg1	12.0 ± 0.5 g/L					286:299	12.0 ± 0.5 g/L	286:299	12.0 ± 0.5 g/L	286:299	The particle size of 0.02 ± 0.01 to 0.1 ± 0.05 mm was rich in glucose (12.0 ± 0.5 g/L), whereas 0.5 ± 0.25 to 2.0 ± 0.5 mm had a high content of xylose (8.0 ± 0.5 g/L).
34358990	2	20	from	rich	269:272	arg1	glucose					277:283	glucose	277:283	glucose (12.0 ± 0.5 g/L)	277:300	The particle size of 0.02 ± 0.01 to 0.1 ± 0.05 mm was rich in glucose (12.0 ± 0.5 g/L), whereas 0.5 ± 0.25 to 2.0 ± 0.5 mm had a high content of xylose (8.0 ± 0.5 g/L).
34358990	3	21	theme	non-detoxified	425:438	arg1	media					467:471	the synthetic, non-detoxified and detoxified hydrolysate media	410:471	the synthetic, non-detoxified and detoxified hydrolysate media	410:471	The xylitol production in the synthetic, non-detoxified and detoxified hydrolysate media was studied (50 ± 0.5 g/L) using 10% v/v non - induced cells of M. caribbica for 120 h.
34358990	4	22	theme	%	702:702	arg1	yields					647:652	xylitol yields	639:652	xylitol yields of 45.00 ± 1.00%, 10.00 ± 1.00% and 54.00 ± 1.00%	639:702	At the end of fermentation with the specific growth rate of 0.056 ± 0.01 (μ), xylitol yields of 45.00 ± 1.00%, 10.00 ± 1.00% and 54.00 ± 1.00% were obtained.
34358990	5	23	dep	2.0 ± 0.5 mm	825:836	arg1	to					822:823	to	822:823	to	822:823	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm could be used as the prospective pretreatment process for ecofriendly and industrial scale production of xylitol with M. caribbica.
34358990	4	24	theme	growth	606:611	arg1	rate					613:616	the specific growth rate	593:616	the specific growth rate of 0.056 ± 0.01 (μ)	593:636	At the end of fermentation with the specific growth rate of 0.056 ± 0.01 (μ), xylitol yields of 45.00 ± 1.00%, 10.00 ± 1.00% and 54.00 ± 1.00% were obtained.
34358990	5	25	theme	identified	775:784	arg1	size					803:806	an identified corncob particle size	772:806	an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm	772:836	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm could be used as the prospective pretreatment process for ecofriendly and industrial scale production of xylitol with M. caribbica.
34358990	0	26	from	hydrolysate	78:88	arg1	composition					31:41	the composition	27:41	the composition of sugars in corncob hemicellulose hydrolysate for xylose fermentation	27:112	Particle size influence on the composition of sugars in corncob hemicellulose hydrolysate for xylose fermentation by Meyerozyma caribbica.
34358990	3	27	theme	caribbica	540:548	arg1	v/v					510:512	10% v/v	506:512	10% v/v non	506:516	The xylitol production in the synthetic, non-detoxified and detoxified hydrolysate media was studied (50 ± 0.5 g/L) using 10% v/v non - induced cells of M. caribbica for 120 h.
34358990	2	28	from	glucose	277:283	arg1	rich					269:272	rich	269:272	rich	269:272	The particle size of 0.02 ± 0.01 to 0.1 ± 0.05 mm was rich in glucose (12.0 ± 0.5 g/L), whereas 0.5 ± 0.25 to 2.0 ± 0.5 mm had a high content of xylose (8.0 ± 0.5 g/L).
34358990	2	28	from	glucose	277:283	arg1	size					228:231	The particle size	215:231	The particle size of 0.02 ± 0.01 to 0.1 ± 0.05 mm	215:263	The particle size of 0.02 ± 0.01 to 0.1 ± 0.05 mm was rich in glucose (12.0 ± 0.5 g/L), whereas 0.5 ± 0.25 to 2.0 ± 0.5 mm had a high content of xylose (8.0 ± 0.5 g/L).
34358990	4	29	theme	xylitol	639:645	arg1	yields					647:652	xylitol yields	639:652	xylitol yields of 45.00 ± 1.00%, 10.00 ± 1.00% and 54.00 ± 1.00%	639:702	At the end of fermentation with the specific growth rate of 0.056 ± 0.01 (μ), xylitol yields of 45.00 ± 1.00%, 10.00 ± 1.00% and 54.00 ± 1.00% were obtained.
34358990	5	30	with	xylitol	943:949	arg1	caribbica					959:967	M. caribbica	956:967	M. caribbica	956:967	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm could be used as the prospective pretreatment process for ecofriendly and industrial scale production of xylitol with M. caribbica.
34358990	5	31	theme	pretreatment	871:882	arg1	detoxification					723:736	The detoxification	719:736	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm	719:836	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm could be used as the prospective pretreatment process for ecofriendly and industrial scale production of xylitol with M. caribbica.
34358990	5	31	theme	pretreatment	871:882	arg1	process					884:890	the prospective pretreatment process	855:890	the prospective pretreatment process	855:890	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm could be used as the prospective pretreatment process for ecofriendly and industrial scale production of xylitol with M. caribbica.
34358990	3	32	theme	hydrolysate	455:465	arg1	media					467:471	the synthetic, non-detoxified and detoxified hydrolysate media	410:471	the synthetic, non-detoxified and detoxified hydrolysate media	410:471	The xylitol production in the synthetic, non-detoxified and detoxified hydrolysate media was studied (50 ± 0.5 g/L) using 10% v/v non - induced cells of M. caribbica for 120 h.
34358990	2	33	theme	0.1 ± 0.05 mm	251:263	arg1	rich					269:272	rich	269:272	rich	269:272	The particle size of 0.02 ± 0.01 to 0.1 ± 0.05 mm was rich in glucose (12.0 ± 0.5 g/L), whereas 0.5 ± 0.25 to 2.0 ± 0.5 mm had a high content of xylose (8.0 ± 0.5 g/L).
34358990	2	33	theme	0.1 ± 0.05 mm	251:263	arg1	size					228:231	The particle size	215:231	The particle size of 0.02 ± 0.01 to 0.1 ± 0.05 mm	215:263	The particle size of 0.02 ± 0.01 to 0.1 ± 0.05 mm was rich in glucose (12.0 ± 0.5 g/L), whereas 0.5 ± 0.25 to 2.0 ± 0.5 mm had a high content of xylose (8.0 ± 0.5 g/L).
34358990	2	34	dep	0.1 ± 0.05 mm	251:263	arg1	to					248:249	to	248:249	to	248:249	The particle size of 0.02 ± 0.01 to 0.1 ± 0.05 mm was rich in glucose (12.0 ± 0.5 g/L), whereas 0.5 ± 0.25 to 2.0 ± 0.5 mm had a high content of xylose (8.0 ± 0.5 g/L).
34358990	3	35	theme	synthetic	414:422	arg1	media					467:471	the synthetic, non-detoxified and detoxified hydrolysate media	410:471	the synthetic, non-detoxified and detoxified hydrolysate media	410:471	The xylitol production in the synthetic, non-detoxified and detoxified hydrolysate media was studied (50 ± 0.5 g/L) using 10% v/v non - induced cells of M. caribbica for 120 h.
34358990	0	36	theme	sugars	46:51	arg1	composition					31:41	the composition	27:41	the composition of sugars in corncob hemicellulose hydrolysate for xylose fermentation	27:112	Particle size influence on the composition of sugars in corncob hemicellulose hydrolysate for xylose fermentation by Meyerozyma caribbica.
34358990	4	37	with	fermentation	575:586	arg1	rate					613:616	the specific growth rate	593:616	the specific growth rate of 0.056 ± 0.01 (μ)	593:636	At the end of fermentation with the specific growth rate of 0.056 ± 0.01 (μ), xylitol yields of 45.00 ± 1.00%, 10.00 ± 1.00% and 54.00 ± 1.00% were obtained.
34358990	4	38	theme	%	669:669	arg1	yields					647:652	xylitol yields	639:652	xylitol yields of 45.00 ± 1.00%, 10.00 ± 1.00% and 54.00 ± 1.00%	639:702	At the end of fermentation with the specific growth rate of 0.056 ± 0.01 (μ), xylitol yields of 45.00 ± 1.00%, 10.00 ± 1.00% and 54.00 ± 1.00% were obtained.
34358990	5	39	theme	hydrolysate	745:755	arg1	detoxification					723:736	The detoxification	719:736	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm	719:836	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm could be used as the prospective pretreatment process for ecofriendly and industrial scale production of xylitol with M. caribbica.
34358990	5	39	theme	hydrolysate	745:755	arg1	process					884:890	the prospective pretreatment process	855:890	the prospective pretreatment process	855:890	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm could be used as the prospective pretreatment process for ecofriendly and industrial scale production of xylitol with M. caribbica.
34358990	3	40	from	production	396:405	arg1	media					467:471	the synthetic, non-detoxified and detoxified hydrolysate media	410:471	the synthetic, non-detoxified and detoxified hydrolysate media	410:471	The xylitol production in the synthetic, non-detoxified and detoxified hydrolysate media was studied (50 ± 0.5 g/L) using 10% v/v non - induced cells of M. caribbica for 120 h.
34358990	3	41	theme	%	508:508	arg1	v/v					510:512	10% v/v	506:512	10% v/v non	506:516	The xylitol production in the synthetic, non-detoxified and detoxified hydrolysate media was studied (50 ± 0.5 g/L) using 10% v/v non - induced cells of M. caribbica for 120 h.
34358990	2	42	theme	high	344:347	arg1	content					349:355	a high content	342:355	a high content of xylose (8.0 ± 0.5 g/L)	342:381	The particle size of 0.02 ± 0.01 to 0.1 ± 0.05 mm was rich in glucose (12.0 ± 0.5 g/L), whereas 0.5 ± 0.25 to 2.0 ± 0.5 mm had a high content of xylose (8.0 ± 0.5 g/L).
34358990	2	43	theme	particle	219:226	arg1	rich					269:272	rich	269:272	rich	269:272	The particle size of 0.02 ± 0.01 to 0.1 ± 0.05 mm was rich in glucose (12.0 ± 0.5 g/L), whereas 0.5 ± 0.25 to 2.0 ± 0.5 mm had a high content of xylose (8.0 ± 0.5 g/L).
34358990	2	43	theme	particle	219:226	arg1	size					228:231	The particle size	215:231	The particle size of 0.02 ± 0.01 to 0.1 ± 0.05 mm	215:263	The particle size of 0.02 ± 0.01 to 0.1 ± 0.05 mm was rich in glucose (12.0 ± 0.5 g/L), whereas 0.5 ± 0.25 to 2.0 ± 0.5 mm had a high content of xylose (8.0 ± 0.5 g/L).
34358990	2	44	dep	2.0 ± 0.5 mm	325:336	arg1	to					322:323	to	322:323	to	322:323	The particle size of 0.02 ± 0.01 to 0.1 ± 0.05 mm was rich in glucose (12.0 ± 0.5 g/L), whereas 0.5 ± 0.25 to 2.0 ± 0.5 mm had a high content of xylose (8.0 ± 0.5 g/L).
34358990	5	45	theme	industrial	912:921	arg1	production					929:938	industrial scale production	912:938	industrial scale production of xylitol with M. caribbica	912:967	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm could be used as the prospective pretreatment process for ecofriendly and industrial scale production of xylitol with M. caribbica.
34358990	4	46	theme	fermentation	575:586	arg1	end					568:570	the end	564:570	the end of fermentation with the specific growth rate of 0.056 ± 0.01 (μ)	564:636	At the end of fermentation with the specific growth rate of 0.056 ± 0.01 (μ), xylitol yields of 45.00 ± 1.00%, 10.00 ± 1.00% and 54.00 ± 1.00% were obtained.
34358990	3	47	theme	induced	520:526	arg1	cells					528:532	induced cells	520:532	induced cells	520:532	The xylitol production in the synthetic, non-detoxified and detoxified hydrolysate media was studied (50 ± 0.5 g/L) using 10% v/v non - induced cells of M. caribbica for 120 h.
34358990	0	48	theme	corncob	56:62	arg1	hydrolysate					78:88	corncob hemicellulose hydrolysate	56:88	corncob hemicellulose hydrolysate for xylose fermentation	56:112	Particle size influence on the composition of sugars in corncob hemicellulose hydrolysate for xylose fermentation by Meyerozyma caribbica.
34358990	5	49	theme	corncob	786:792	arg1	size					803:806	an identified corncob particle size	772:806	an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm	772:836	The detoxification of the hydrolysate prepared using an identified corncob particle size of 0.5 ± 0.25 to 2.0 ± 0.5 mm could be used as the prospective pretreatment process for ecofriendly and industrial scale production of xylitol with M. caribbica.
34358990	1	50	theme	xylitol	143:149	arg1	production					151:160	The xylitol production	139:160	The xylitol production	139:160	The xylitol production was performed with acidophilic Meyerozyma caribbica.
34358990	3	51	theme	detoxified	444:453	arg1	media					467:471	the synthetic, non-detoxified and detoxified hydrolysate media	410:471	the synthetic, non-detoxified and detoxified hydrolysate media	410:471	The xylitol production in the synthetic, non-detoxified and detoxified hydrolysate media was studied (50 ± 0.5 g/L) using 10% v/v non - induced cells of M. caribbica for 120 h.
34358990	2	52	contain	had	338:340	arg2	content					349:355	a high content	342:355	a high content of xylose (8.0 ± 0.5 g/L)	342:381	The particle size of 0.02 ± 0.01 to 0.1 ± 0.05 mm was rich in glucose (12.0 ± 0.5 g/L), whereas 0.5 ± 0.25 to 2.0 ± 0.5 mm had a high content of xylose (8.0 ± 0.5 g/L).
34358990	2	52	contain	had	338:340	arg1	2.0 ± 0.5 mm					325:336	2.0 ± 0.5 mm	325:336	2.0 ± 0.5 mm	325:336	The particle size of 0.02 ± 0.01 to 0.1 ± 0.05 mm was rich in glucose (12.0 ± 0.5 g/L), whereas 0.5 ± 0.25 to 2.0 ± 0.5 mm had a high content of xylose (8.0 ± 0.5 g/L).
34358990	3	53	dep	studied	477:483	arg1	50 ± 0.5 g/L					486:497	50 ± 0.5 g/L	486:497	50 ± 0.5 g/L	486:497	The xylitol production in the synthetic, non-detoxified and detoxified hydrolysate media was studied (50 ± 0.5 g/L) using 10% v/v non - induced cells of M. caribbica for 120 h.
34358990	3	54	theme	non	514:516	arg1	v/v					510:512	10% v/v	506:512	10% v/v non	506:516	The xylitol production in the synthetic, non-detoxified and detoxified hydrolysate media was studied (50 ± 0.5 g/L) using 10% v/v non - induced cells of M. caribbica for 120 h.
34022307	4	0	theme	residues	840:847	arg1	O-5					816:818	O-5	816:818	O-5 of arabinofuranosyl residues	816:847	The highly active polymer (HSV-1: IC50 and SI, respectively, of 127 μg/mL and 15.7) was a 69 kDa arabinogalactan holding sulfates at O-5 of arabinofuranosyl residues and showed no cytotoxicity as far as 2 mg/mL concentration.
34022307	5	1	theme	sulfated	925:932	arg1	macromolecule					934:946	This chemically sulfated macromolecule	909:946	This chemically sulfated macromolecule	909:946	This chemically sulfated macromolecule acted by obstructing viral attachment and entry.
34022307	2	2	from	gum	464:466	arg1	sulfates					429:436	arabinogalactan sulfates	413:436	arabinogalactan sulfates from Anogeissus latifolia gum	413:466	In this work, we utilized a sulfur trioxide-pyridine in N,N-dimethylformamide (SO3·Pyr/DMF) based amalgamated extraction-sulfation procedure for producing arabinogalactan sulfates from Anogeissus latifolia gum.
34022307	4	3	theme	arabinofuranosyl	823:838	arg1	residues					840:847	arabinofuranosyl residues	823:847	arabinofuranosyl residues	823:847	The highly active polymer (HSV-1: IC50 and SI, respectively, of 127 μg/mL and 15.7) was a 69 kDa arabinogalactan holding sulfates at O-5 of arabinofuranosyl residues and showed no cytotoxicity as far as 2 mg/mL concentration.
34022307	6	4	theme	altered	1082:1088	arg1	activities					1106:1115	altered pharmacological activities	1082:1115	altered pharmacological activities	1082:1115	Thus, SO3·Pyr/DMF is suitable for producing new molecules with varied structures and altered pharmacological activities from plant sources.
34022307	1	5	theme	susceptible	239:249	arg1	cells					251:255	susceptible cells	239:255	susceptible cells	239:255	Herpes simplex virus type 1(HSV-1) attaches to cell surface heparan sulfate aiming to enter into susceptible cells.
34022307	3	6	link	derived	546:552	arg1	polymers					554:561	the derived polymers	542:561	the derived polymers	542:561	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	2	7	from	trioxide-pyridine	293:309	arg1	N					314:314	N	314:314	N	314:314	In this work, we utilized a sulfur trioxide-pyridine in N,N-dimethylformamide (SO3·Pyr/DMF) based amalgamated extraction-sulfation procedure for producing arabinogalactan sulfates from Anogeissus latifolia gum.
34022307	2	7	from	trioxide-pyridine	293:309	arg1	N-dimethylformamide					316:334	N-dimethylformamide	316:334	N-dimethylformamide (SO3·Pyr/DMF)	316:348	In this work, we utilized a sulfur trioxide-pyridine in N,N-dimethylformamide (SO3·Pyr/DMF) based amalgamated extraction-sulfation procedure for producing arabinogalactan sulfates from Anogeissus latifolia gum.
34022307	4	8	dep	HSV-1	710:714	arg1	SI					726:727	SI	726:727	SI	726:727	The highly active polymer (HSV-1: IC50 and SI, respectively, of 127 μg/mL and 15.7) was a 69 kDa arabinogalactan holding sulfates at O-5 of arabinofuranosyl residues and showed no cytotoxicity as far as 2 mg/mL concentration.
34022307	4	8	dep	HSV-1	710:714	arg1	IC50					717:720	IC50	717:720	IC50	717:720	The highly active polymer (HSV-1: IC50 and SI, respectively, of 127 μg/mL and 15.7) was a 69 kDa arabinogalactan holding sulfates at O-5 of arabinofuranosyl residues and showed no cytotoxicity as far as 2 mg/mL concentration.
34022307	0	9	from	gum	73:75	arg1	sulfates					38:45	The heparin-mimicking arabinogalactan sulfates	0:45	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.	0:140	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	0	9	from	gum	73:75	arg1	activity					132:139	anti-herpes simplex virus activity	106:139	anti-herpes simplex virus activity	106:139	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	0	9	from	gum	73:75	arg1	Production					78:87	Production	78:87	Production	78:87	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	4	10	dep	polymer	701:707	arg1	HSV-1					710:714	HSV-1	710:714	HSV-1	710:714	The highly active polymer (HSV-1: IC50 and SI, respectively, of 127 μg/mL and 15.7) was a 69 kDa arabinogalactan holding sulfates at O-5 of arabinofuranosyl residues and showed no cytotoxicity as far as 2 mg/mL concentration.
34022307	3	11	theme	varying	573:579	arg1	masses					591:596	varying molecular masses	573:596	varying molecular masses (31-69 kDa)	573:608	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	3	11	theme	varying	573:579	arg1	kDa					605:607	31-69 kDa	599:607	31-69 kDa	599:607	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	3	11	theme	varying	573:579	arg1	0.1-0.5					636:642	0.1-0.5	636:642	0.1-0.5	636:642	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	3	12	theme	spectroscopic	496:508	arg1	data					523:526	Chemical, chromatographic, spectroscopic and chemical data	469:526	Chemical, chromatographic, spectroscopic and chemical data	469:526	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	0	13	theme	simplex	118:124	arg1	sulfates					38:45	The heparin-mimicking arabinogalactan sulfates	0:45	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.	0:140	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	0	13	theme	simplex	118:124	arg1	activity					132:139	anti-herpes simplex virus activity	106:139	anti-herpes simplex virus activity	106:139	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	2	14	dep	Anogeissus	443:452	arg1	latifolia					454:462	latifolia	454:462	latifolia	454:462	In this work, we utilized a sulfur trioxide-pyridine in N,N-dimethylformamide (SO3·Pyr/DMF) based amalgamated extraction-sulfation procedure for producing arabinogalactan sulfates from Anogeissus latifolia gum.
34022307	2	15	theme	arabinogalactan	413:427	arg1	sulfates					429:436	arabinogalactan sulfates	413:436	arabinogalactan sulfates from Anogeissus latifolia gum	413:466	In this work, we utilized a sulfur trioxide-pyridine in N,N-dimethylformamide (SO3·Pyr/DMF) based amalgamated extraction-sulfation procedure for producing arabinogalactan sulfates from Anogeissus latifolia gum.
34022307	3	16	theme	derived	546:552	arg1	polymers					554:561	the derived polymers	542:561	the derived polymers	542:561	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	6	17	theme	varied	1060:1065	arg1	structures					1067:1076	varied structures	1060:1076	varied structures	1060:1076	Thus, SO3·Pyr/DMF is suitable for producing new molecules with varied structures and altered pharmacological activities from plant sources.
34022307	0	18	theme	anti-herpes	106:116	arg1	sulfates					38:45	The heparin-mimicking arabinogalactan sulfates	0:45	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.	0:140	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	0	18	theme	anti-herpes	106:116	arg1	activity					132:139	anti-herpes simplex virus activity	106:139	anti-herpes simplex virus activity	106:139	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	4	19	theme	μg/mL	751:755	arg1	HSV-1					710:714	HSV-1	710:714	HSV-1	710:714	The highly active polymer (HSV-1: IC50 and SI, respectively, of 127 μg/mL and 15.7) was a 69 kDa arabinogalactan holding sulfates at O-5 of arabinofuranosyl residues and showed no cytotoxicity as far as 2 mg/mL concentration.
34022307	2	20	theme	extraction-sulfation	368:387	arg1	procedure					389:397	based amalgamated extraction-sulfation procedure	350:397	based amalgamated extraction-sulfation procedure	350:397	In this work, we utilized a sulfur trioxide-pyridine in N,N-dimethylformamide (SO3·Pyr/DMF) based amalgamated extraction-sulfation procedure for producing arabinogalactan sulfates from Anogeissus latifolia gum.
34022307	0	21	theme	heparin-mimicking	4:20	arg1	sulfates					38:45	The heparin-mimicking arabinogalactan sulfates	0:45	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.	0:140	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	0	21	theme	heparin-mimicking	4:20	arg1	activity					132:139	anti-herpes simplex virus activity	106:139	anti-herpes simplex virus activity	106:139	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	0	21	theme	heparin-mimicking	4:20	arg1	Production					78:87	Production	78:87	Production	78:87	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	2	22	theme	sulfur	286:291	arg1	trioxide-pyridine					293:309	a sulfur trioxide-pyridine	284:309	a sulfur trioxide-pyridine in N,N-dimethylformamide (SO3·Pyr/DMF) based amalgamated extraction-sulfation procedure for producing arabinogalactan sulfates from Anogeissus latifolia gum	284:466	In this work, we utilized a sulfur trioxide-pyridine in N,N-dimethylformamide (SO3·Pyr/DMF) based amalgamated extraction-sulfation procedure for producing arabinogalactan sulfates from Anogeissus latifolia gum.
34022307	2	23	theme	amalgamated	356:366	arg1	procedure					389:397	based amalgamated extraction-sulfation procedure	350:397	based amalgamated extraction-sulfation procedure	350:397	In this work, we utilized a sulfur trioxide-pyridine in N,N-dimethylformamide (SO3·Pyr/DMF) based amalgamated extraction-sulfation procedure for producing arabinogalactan sulfates from Anogeissus latifolia gum.
34022307	2	24	theme	based	350:354	arg1	procedure					389:397	based amalgamated extraction-sulfation procedure	350:397	based amalgamated extraction-sulfation procedure	350:397	In this work, we utilized a sulfur trioxide-pyridine in N,N-dimethylformamide (SO3·Pyr/DMF) based amalgamated extraction-sulfation procedure for producing arabinogalactan sulfates from Anogeissus latifolia gum.
34022307	2	25	theme	Anogeissus	443:452	arg1	gum					464:466	Anogeissus latifolia gum	443:466	Anogeissus latifolia gum	443:466	In this work, we utilized a sulfur trioxide-pyridine in N,N-dimethylformamide (SO3·Pyr/DMF) based amalgamated extraction-sulfation procedure for producing arabinogalactan sulfates from Anogeissus latifolia gum.
34022307	3	26	theme	molecular	581:589	arg1	masses					591:596	varying molecular masses	573:596	varying molecular masses (31-69 kDa)	573:608	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	3	26	theme	molecular	581:589	arg1	kDa					605:607	31-69 kDa	599:607	31-69 kDa	599:607	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	3	26	theme	molecular	581:589	arg1	0.1-0.5					636:642	0.1-0.5	636:642	0.1-0.5	636:642	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	0	27	theme	arabinogalactan	22:36	arg1	sulfates					38:45	The heparin-mimicking arabinogalactan sulfates	0:45	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.	0:140	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	0	27	theme	arabinogalactan	22:36	arg1	activity					132:139	anti-herpes simplex virus activity	106:139	anti-herpes simplex virus activity	106:139	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	0	27	theme	arabinogalactan	22:36	arg1	Production					78:87	Production	78:87	Production	78:87	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	0	28	theme	virus	126:130	arg1	sulfates					38:45	The heparin-mimicking arabinogalactan sulfates	0:45	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.	0:140	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	0	28	theme	virus	126:130	arg1	activity					132:139	anti-herpes simplex virus activity	106:139	anti-herpes simplex virus activity	106:139	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	2	29	dep	N	314:314	arg1	procedure					389:397	based amalgamated extraction-sulfation procedure	350:397	based amalgamated extraction-sulfation procedure	350:397	In this work, we utilized a sulfur trioxide-pyridine in N,N-dimethylformamide (SO3·Pyr/DMF) based amalgamated extraction-sulfation procedure for producing arabinogalactan sulfates from Anogeissus latifolia gum.
34022307	1	30	theme	cell	189:192	arg1	sulfate					210:216	cell surface heparan sulfate	189:216	cell surface heparan sulfate aiming to enter into susceptible cells	189:255	Herpes simplex virus type 1(HSV-1) attaches to cell surface heparan sulfate aiming to enter into susceptible cells.
34022307	0	31	theme	Anogeissus	52:61	arg1	gum					73:75	Anogeissus latifolia gum	52:75	Anogeissus latifolia gum	52:75	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	6	32	theme	plant	1122:1126	arg1	sources					1128:1134	plant sources	1122:1134	plant sources	1122:1134	Thus, SO3·Pyr/DMF is suitable for producing new molecules with varied structures and altered pharmacological activities from plant sources.
34022307	3	33	theme	similar	650:656	arg1	compositions					669:680	similar saccharide compositions	650:680	similar saccharide compositions	650:680	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	4	34	theme	kDa	776:778	arg1	polymer					701:707	The highly active polymer	683:707	The highly active polymer (HSV-1: IC50 and SI, respectively, of 127 μg/mL and 15.7)	683:765	The highly active polymer (HSV-1: IC50 and SI, respectively, of 127 μg/mL and 15.7) was a 69 kDa arabinogalactan holding sulfates at O-5 of arabinofuranosyl residues and showed no cytotoxicity as far as 2 mg/mL concentration.
34022307	4	34	theme	kDa	776:778	arg1	arabinogalactan					780:794	a 69 kDa arabinogalactan	771:794	a 69 kDa arabinogalactan holding sulfates at O-5 of arabinofuranosyl residues	771:847	The highly active polymer (HSV-1: IC50 and SI, respectively, of 127 μg/mL and 15.7) was a 69 kDa arabinogalactan holding sulfates at O-5 of arabinofuranosyl residues and showed no cytotoxicity as far as 2 mg/mL concentration.
34022307	6	35	theme	new	1041:1043	arg1	molecules					1045:1053	new molecules	1041:1053	new molecules	1041:1053	Thus, SO3·Pyr/DMF is suitable for producing new molecules with varied structures and altered pharmacological activities from plant sources.
34022307	1	36	theme	surface	194:200	arg1	sulfate					210:216	cell surface heparan sulfate	189:216	cell surface heparan sulfate aiming to enter into susceptible cells	189:255	Herpes simplex virus type 1(HSV-1) attaches to cell surface heparan sulfate aiming to enter into susceptible cells.
34022307	4	37	theme	active	694:699	arg1	polymer					701:707	The highly active polymer	683:707	The highly active polymer (HSV-1: IC50 and SI, respectively, of 127 μg/mL and 15.7)	683:765	The highly active polymer (HSV-1: IC50 and SI, respectively, of 127 μg/mL and 15.7) was a 69 kDa arabinogalactan holding sulfates at O-5 of arabinofuranosyl residues and showed no cytotoxicity as far as 2 mg/mL concentration.
34022307	4	37	theme	active	694:699	arg1	arabinogalactan					780:794	a 69 kDa arabinogalactan	771:794	a 69 kDa arabinogalactan holding sulfates at O-5 of arabinofuranosyl residues	771:847	The highly active polymer (HSV-1: IC50 and SI, respectively, of 127 μg/mL and 15.7) was a 69 kDa arabinogalactan holding sulfates at O-5 of arabinofuranosyl residues and showed no cytotoxicity as far as 2 mg/mL concentration.
34022307	4	38	theme	mg/mL	888:892	arg1	concentration					894:906	2 mg/mL concentration	886:906	no cytotoxicity as far as 2 mg/mL concentration	860:906	The highly active polymer (HSV-1: IC50 and SI, respectively, of 127 μg/mL and 15.7) was a 69 kDa arabinogalactan holding sulfates at O-5 of arabinofuranosyl residues and showed no cytotoxicity as far as 2 mg/mL concentration.
34022307	1	39	attach	attaches	177:184	arg1	sulfate					210:216	cell surface heparan sulfate	189:216	cell surface heparan sulfate aiming to enter into susceptible cells	189:255	Herpes simplex virus type 1(HSV-1) attaches to cell surface heparan sulfate aiming to enter into susceptible cells.
34022307	1	39	attach	attaches	177:184	arg2	type					163:166	Herpes simplex virus type 1	142:168	Herpes simplex virus type 1(HSV-1)	142:175	Herpes simplex virus type 1(HSV-1) attaches to cell surface heparan sulfate aiming to enter into susceptible cells.
34022307	1	39	attach	attaches	177:184	arg2	HSV-1					170:174	HSV-1	170:174	HSV-1	170:174	Herpes simplex virus type 1(HSV-1) attaches to cell surface heparan sulfate aiming to enter into susceptible cells.
34022307	4	40	theme	69	773:774	arg1	kDa					776:778	kDa	776:778	kDa	776:778	The highly active polymer (HSV-1: IC50 and SI, respectively, of 127 μg/mL and 15.7) was a 69 kDa arabinogalactan holding sulfates at O-5 of arabinofuranosyl residues and showed no cytotoxicity as far as 2 mg/mL concentration.
34022307	1	41	theme	heparan	202:208	arg1	sulfate					210:216	cell surface heparan sulfate	189:216	cell surface heparan sulfate aiming to enter into susceptible cells	189:255	Herpes simplex virus type 1(HSV-1) attaches to cell surface heparan sulfate aiming to enter into susceptible cells.
34022307	0	42	dep	Anogeissus	52:61	arg1	latifolia					63:71	latifolia	63:71	latifolia	63:71	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	6	43	theme	pharmacological	1090:1104	arg1	activities					1106:1115	altered pharmacological activities	1082:1115	altered pharmacological activities	1082:1115	Thus, SO3·Pyr/DMF is suitable for producing new molecules with varied structures and altered pharmacological activities from plant sources.
34022307	3	44	theme	chromatographic	479:493	arg1	data					523:526	Chemical, chromatographic, spectroscopic and chemical data	469:526	Chemical, chromatographic, spectroscopic and chemical data	469:526	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	6	45	from	sources	1128:1134	arg1	structures					1067:1076	varied structures	1060:1076	varied structures	1060:1076	Thus, SO3·Pyr/DMF is suitable for producing new molecules with varied structures and altered pharmacological activities from plant sources.
34022307	6	45	from	sources	1128:1134	arg1	activities					1106:1115	altered pharmacological activities	1082:1115	altered pharmacological activities	1082:1115	Thus, SO3·Pyr/DMF is suitable for producing new molecules with varied structures and altered pharmacological activities from plant sources.
34022307	3	46	theme	chemical	514:521	arg1	data					523:526	Chemical, chromatographic, spectroscopic and chemical data	469:526	Chemical, chromatographic, spectroscopic and chemical data	469:526	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	3	47	contain	contained	563:571	arg2	0.1-0.5					636:642	0.1-0.5	636:642	0.1-0.5	636:642	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	3	47	contain	contained	563:571	arg2	compositions					669:680	similar saccharide compositions	650:680	similar saccharide compositions	650:680	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	3	47	contain	contained	563:571	arg2	kDa					605:607	31-69 kDa	599:607	31-69 kDa	599:607	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	3	47	contain	contained	563:571	arg2	masses					591:596	varying molecular masses	573:596	varying molecular masses (31-69 kDa)	573:608	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	3	47	contain	contained	563:571	arg2	degrees					614:620	degrees	614:620	degrees of sulfation	614:633	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	3	47	contain	contained	563:571	arg1	polymers					554:561	the derived polymers	542:561	the derived polymers	542:561	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	5	48	theme	viral	969:973	arg1	attachment					975:984	viral attachment	969:984	viral attachment	969:984	This chemically sulfated macromolecule acted by obstructing viral attachment and entry.
34022307	0	49	dep	sulfates	38:45	arg1	structures					90:99	structures	90:99	structures	90:99	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	0	49	dep	sulfates	38:45	arg1	activity					132:139	anti-herpes simplex virus activity	106:139	anti-herpes simplex virus activity	106:139	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	0	49	dep	sulfates	38:45	arg1	sulfates					38:45	The heparin-mimicking arabinogalactan sulfates	0:45	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.	0:140	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	0	49	dep	sulfates	38:45	arg1	Production					78:87	Production	78:87	Production	78:87	The heparin-mimicking arabinogalactan sulfates from Anogeissus latifolia gum: Production, structures, and anti-herpes simplex virus activity.
34022307	2	50	used	utilized	275:282	arg2	we					272:273	we	272:273	we	272:273	In this work, we utilized a sulfur trioxide-pyridine in N,N-dimethylformamide (SO3·Pyr/DMF) based amalgamated extraction-sulfation procedure for producing arabinogalactan sulfates from Anogeissus latifolia gum.
34022307	3	51	theme	saccharide	658:667	arg1	compositions					669:680	similar saccharide compositions	650:680	similar saccharide compositions	650:680	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	4	52	theme	15.7	761:764	arg1	HSV-1					710:714	HSV-1	710:714	HSV-1	710:714	The highly active polymer (HSV-1: IC50 and SI, respectively, of 127 μg/mL and 15.7) was a 69 kDa arabinogalactan holding sulfates at O-5 of arabinofuranosyl residues and showed no cytotoxicity as far as 2 mg/mL concentration.
34022307	1	53	theme	Herpes	142:147	arg1	type					163:166	Herpes simplex virus type 1	142:168	Herpes simplex virus type 1(HSV-1)	142:175	Herpes simplex virus type 1(HSV-1) attaches to cell surface heparan sulfate aiming to enter into susceptible cells.
34022307	1	53	theme	Herpes	142:147	arg1	HSV-1					170:174	HSV-1	170:174	HSV-1	170:174	Herpes simplex virus type 1(HSV-1) attaches to cell surface heparan sulfate aiming to enter into susceptible cells.
34022307	4	54	dep	far	879:881	arg1	as					883:884	as	883:884	as	883:884	The highly active polymer (HSV-1: IC50 and SI, respectively, of 127 μg/mL and 15.7) was a 69 kDa arabinogalactan holding sulfates at O-5 of arabinofuranosyl residues and showed no cytotoxicity as far as 2 mg/mL concentration.
34022307	3	55	theme	Chemical	469:476	arg1	data					523:526	Chemical, chromatographic, spectroscopic and chemical data	469:526	Chemical, chromatographic, spectroscopic and chemical data	469:526	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	1	56	theme	simplex	149:155	arg1	type					163:166	Herpes simplex virus type 1	142:168	Herpes simplex virus type 1(HSV-1)	142:175	Herpes simplex virus type 1(HSV-1) attaches to cell surface heparan sulfate aiming to enter into susceptible cells.
34022307	1	56	theme	simplex	149:155	arg1	HSV-1					170:174	HSV-1	170:174	HSV-1	170:174	Herpes simplex virus type 1(HSV-1) attaches to cell surface heparan sulfate aiming to enter into susceptible cells.
34022307	3	57	theme	sulfation	625:633	arg1	masses					591:596	varying molecular masses	573:596	varying molecular masses (31-69 kDa)	573:608	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	3	57	theme	sulfation	625:633	arg1	kDa					605:607	31-69 kDa	599:607	31-69 kDa	599:607	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	3	57	theme	sulfation	625:633	arg1	degrees					614:620	degrees	614:620	degrees of sulfation	614:633	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	3	57	theme	sulfation	625:633	arg1	0.1-0.5					636:642	0.1-0.5	636:642	0.1-0.5	636:642	Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions.
34022307	1	58	theme	virus	157:161	arg1	type					163:166	Herpes simplex virus type 1	142:168	Herpes simplex virus type 1(HSV-1)	142:175	Herpes simplex virus type 1(HSV-1) attaches to cell surface heparan sulfate aiming to enter into susceptible cells.
34022307	1	58	theme	virus	157:161	arg1	HSV-1					170:174	HSV-1	170:174	HSV-1	170:174	Herpes simplex virus type 1(HSV-1) attaches to cell surface heparan sulfate aiming to enter into susceptible cells.
32204004	5	0	theme	composition	844:854	arg1	scaffolds					932:940	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds	808:940	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds	808:940	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds were thoroughly investigated.
32204004	6	1	theme	high	1018:1021	arg1	porosity					1023:1030	a high porosity	1016:1030	a high porosity	1016:1030	The as-prepared nHAp/CS scaffolds exhibited a high porosity and excellent swelling performance.
32204004	3	2	theme	colloidal	513:521	arg1	solution					523:530	the homogenously dispersed nano-hydroxyapatite (nHAp) colloidal solution	459:530	the homogenously dispersed nano-hydroxyapatite (nHAp) colloidal solution	459:530	In this study, the homogenously dispersed nano-hydroxyapatite (nHAp) colloidal solution was first synthesized using a co-precipitation method.
32204004	7	3	theme	higher	1149:1154	arg1	modulus					1168:1174	higher compressive modulus	1149:1174	higher compressive modulus	1149:1174	Compared with pure CS scaffolds, the nHAp/CS composite scaffolds not only showed higher compressive modulus but also exhibited better biocompatibility.
32204004	4	4	theme	nano-hydroxyapatite/chitosan	616:643	arg1	scaffolds					665:673	The three-dimensional porous nano-hydroxyapatite/chitosan (nHAp/CS) composite scaffolds	587:673	The three-dimensional porous nano-hydroxyapatite/chitosan (nHAp/CS) composite scaffolds with different nHAp contents	587:702	The three-dimensional porous nano-hydroxyapatite/chitosan (nHAp/CS) composite scaffolds with different nHAp contents were then obtained through an environmentally friendly freeze-drying process without any cross-linking.
32204004	8	5	theme	friendly	1269:1276	arg1	technique					1278:1286	a simple and environmentally friendly technique	1240:1286	a simple and environmentally friendly technique to construct three-dimensional porous nHAp/CS composite scaffolds, which demonstrate promising potential by being a scaffold material for bone tissue engineering	1240:1448	This study provides a simple and environmentally friendly technique to construct three-dimensional porous nHAp/CS composite scaffolds, which demonstrate promising potential by being a scaffold material for bone tissue engineering.
32204004	8	6	theme	promising	1373:1381	arg1	potential					1383:1391	promising potential	1373:1391	promising potential	1373:1391	This study provides a simple and environmentally friendly technique to construct three-dimensional porous nHAp/CS composite scaffolds, which demonstrate promising potential by being a scaffold material for bone tissue engineering.
32204004	4	7	theme	freeze-drying	759:771	arg1	process					773:779	an environmentally friendly freeze-drying process	731:779	an environmentally friendly freeze-drying process without any cross-linking	731:805	The three-dimensional porous nano-hydroxyapatite/chitosan (nHAp/CS) composite scaffolds with different nHAp contents were then obtained through an environmentally friendly freeze-drying process without any cross-linking.
32204004	4	8	theme	porous	609:614	arg1	scaffolds					665:673	The three-dimensional porous nano-hydroxyapatite/chitosan (nHAp/CS) composite scaffolds	587:673	The three-dimensional porous nano-hydroxyapatite/chitosan (nHAp/CS) composite scaffolds with different nHAp contents	587:702	The three-dimensional porous nano-hydroxyapatite/chitosan (nHAp/CS) composite scaffolds with different nHAp contents were then obtained through an environmentally friendly freeze-drying process without any cross-linking.
32204004	7	9	theme	pure	1082:1085	arg1	scaffolds					1090:1098	pure CS scaffolds	1082:1098	pure CS scaffolds	1082:1098	Compared with pure CS scaffolds, the nHAp/CS composite scaffolds not only showed higher compressive modulus but also exhibited better biocompatibility.
32204004	4	10	theme	nHAp/CS	646:652	arg1	scaffolds					665:673	The three-dimensional porous nano-hydroxyapatite/chitosan (nHAp/CS) composite scaffolds	587:673	The three-dimensional porous nano-hydroxyapatite/chitosan (nHAp/CS) composite scaffolds with different nHAp contents	587:702	The three-dimensional porous nano-hydroxyapatite/chitosan (nHAp/CS) composite scaffolds with different nHAp contents were then obtained through an environmentally friendly freeze-drying process without any cross-linking.
32204004	0	11	theme	reinforcing	87:97	arg1	filler					99:104	a reinforcing filler	85:104	a reinforcing filler for chitosan	85:117	Preparation and properties of a highly dispersed nano-hydroxyapatite colloid used as a reinforcing filler for chitosan.
32204004	4	12	theme	different	680:688	arg1	contents					695:702	different nHAp contents	680:702	different nHAp contents	680:702	The three-dimensional porous nano-hydroxyapatite/chitosan (nHAp/CS) composite scaffolds with different nHAp contents were then obtained through an environmentally friendly freeze-drying process without any cross-linking.
32204004	3	13	theme	co-precipitation	562:577	arg1	method					579:584	a co-precipitation method	560:584	a co-precipitation method	560:584	In this study, the homogenously dispersed nano-hydroxyapatite (nHAp) colloidal solution was first synthesized using a co-precipitation method.
32204004	8	14	theme	porous	1319:1324	arg1	scaffolds					1344:1352	three-dimensional porous nHAp/CS composite scaffolds	1301:1352	three-dimensional porous nHAp/CS composite scaffolds	1301:1352	This study provides a simple and environmentally friendly technique to construct three-dimensional porous nHAp/CS composite scaffolds, which demonstrate promising potential by being a scaffold material for bone tissue engineering.
32204004	6	15	theme	nHAp/CS	988:994	arg1	scaffolds					996:1004	The as-prepared nHAp/CS scaffolds	972:1004	The as-prepared nHAp/CS scaffolds	972:1004	The as-prepared nHAp/CS scaffolds exhibited a high porosity and excellent swelling performance.
32204004	3	16	theme	dispersed	476:484	arg1	solution					523:530	the homogenously dispersed nano-hydroxyapatite (nHAp) colloidal solution	459:530	the homogenously dispersed nano-hydroxyapatite (nHAp) colloidal solution	459:530	In this study, the homogenously dispersed nano-hydroxyapatite (nHAp) colloidal solution was first synthesized using a co-precipitation method.
32204004	5	17	theme	porosity	828:835	arg1	scaffolds					932:940	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds	808:940	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds	808:940	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds were thoroughly investigated.
32204004	6	18	theme	as-prepared	976:986	arg1	scaffolds					996:1004	The as-prepared nHAp/CS scaffolds	972:1004	The as-prepared nHAp/CS scaffolds	972:1004	The as-prepared nHAp/CS scaffolds exhibited a high porosity and excellent swelling performance.
32204004	5	19	theme	properties	884:893	arg1	scaffolds					932:940	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds	808:940	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds	808:940	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds were thoroughly investigated.
32204004	7	20	theme	better	1195:1200	arg1	biocompatibility					1202:1217	better biocompatibility	1195:1217	better biocompatibility	1195:1217	Compared with pure CS scaffolds, the nHAp/CS composite scaffolds not only showed higher compressive modulus but also exhibited better biocompatibility.
32204004	8	21	theme	scaffold	1404:1411	arg1	material					1413:1420	a scaffold material	1402:1420	a scaffold material for bone tissue engineering	1402:1448	This study provides a simple and environmentally friendly technique to construct three-dimensional porous nHAp/CS composite scaffolds, which demonstrate promising potential by being a scaffold material for bone tissue engineering.
32204004	8	22	theme	nHAp/CS	1326:1332	arg1	scaffolds					1344:1352	three-dimensional porous nHAp/CS composite scaffolds	1301:1352	three-dimensional porous nHAp/CS composite scaffolds	1301:1352	This study provides a simple and environmentally friendly technique to construct three-dimensional porous nHAp/CS composite scaffolds, which demonstrate promising potential by being a scaffold material for bone tissue engineering.
32204004	8	23	theme	three-dimensional	1301:1317	arg1	scaffolds					1344:1352	three-dimensional porous nHAp/CS composite scaffolds	1301:1352	three-dimensional porous nHAp/CS composite scaffolds	1301:1352	This study provides a simple and environmentally friendly technique to construct three-dimensional porous nHAp/CS composite scaffolds, which demonstrate promising potential by being a scaffold material for bone tissue engineering.
32204004	2	24	theme	mechanical	313:322	arg1	properties					324:333	the mechanical properties	309:333	the mechanical properties of CS	309:339	However, to improve the mechanical properties of CS, cross-linking agents are commonly added, which will seriously affect its biocompatibility and safety.
32204004	4	25	theme	nHAp	690:693	arg1	contents					695:702	different nHAp contents	680:702	different nHAp contents	680:702	The three-dimensional porous nano-hydroxyapatite/chitosan (nHAp/CS) composite scaffolds with different nHAp contents were then obtained through an environmentally friendly freeze-drying process without any cross-linking.
32204004	5	26	theme	biocompatibility	900:915	arg1	scaffolds					932:940	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds	808:940	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds	808:940	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds were thoroughly investigated.
32204004	4	27	theme	three-dimensional	591:607	arg1	scaffolds					665:673	The three-dimensional porous nano-hydroxyapatite/chitosan (nHAp/CS) composite scaffolds	587:673	The three-dimensional porous nano-hydroxyapatite/chitosan (nHAp/CS) composite scaffolds with different nHAp contents	587:702	The three-dimensional porous nano-hydroxyapatite/chitosan (nHAp/CS) composite scaffolds with different nHAp contents were then obtained through an environmentally friendly freeze-drying process without any cross-linking.
32204004	1	28	theme	tissue	204:209	arg1	engineering					211:221	tissue engineering	204:221	tissue engineering	204:221	Hydroxyapatite/chitosan (HAp/CS) composites have been widely studied and applied in tissue engineering fields due to their excellent biocompatibility and degradability.
32204004	5	29	theme	swelling	857:864	arg1	ratio					866:870	swelling ratio	857:870	swelling ratio	857:870	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds were thoroughly investigated.
32204004	2	30	theme	cross-linking	342:354	arg1	agents					356:361	cross-linking agents	342:361	cross-linking agents	342:361	However, to improve the mechanical properties of CS, cross-linking agents are commonly added, which will seriously affect its biocompatibility and safety.
32204004	8	31	theme	tissue	1431:1436	arg1	engineering					1438:1448	bone tissue engineering	1426:1448	bone tissue engineering	1426:1448	This study provides a simple and environmentally friendly technique to construct three-dimensional porous nHAp/CS composite scaffolds, which demonstrate promising potential by being a scaffold material for bone tissue engineering.
32204004	5	32	theme	nHAp/CS	924:930	arg1	composition					844:854	phase composition	838:854	phase composition	838:854	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds were thoroughly investigated.
32204004	5	32	theme	nHAp/CS	924:930	arg1	properties					884:893	mechanical properties	873:893	mechanical properties	873:893	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds were thoroughly investigated.
32204004	5	32	theme	nHAp/CS	924:930	arg1	microstructure					812:825	microstructure	812:825	microstructure	812:825	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds were thoroughly investigated.
32204004	5	32	theme	nHAp/CS	924:930	arg1	biocompatibility					900:915	biocompatibility	900:915	biocompatibility of the nHAp/CS	900:930	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds were thoroughly investigated.
32204004	5	32	theme	nHAp/CS	924:930	arg1	porosity					828:835	porosity	828:835	porosity	828:835	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds were thoroughly investigated.
32204004	5	32	theme	nHAp/CS	924:930	arg1	ratio					866:870	swelling ratio	857:870	swelling ratio	857:870	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds were thoroughly investigated.
32204004	5	33	theme	ratio	866:870	arg1	scaffolds					932:940	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds	808:940	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds	808:940	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds were thoroughly investigated.
32204004	5	34	theme	microstructure	812:825	arg1	scaffolds					932:940	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds	808:940	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds	808:940	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds were thoroughly investigated.
32204004	4	35	with	scaffolds	665:673	arg1	contents					695:702	different nHAp contents	680:702	different nHAp contents	680:702	The three-dimensional porous nano-hydroxyapatite/chitosan (nHAp/CS) composite scaffolds with different nHAp contents were then obtained through an environmentally friendly freeze-drying process without any cross-linking.
32204004	8	36	theme	bone	1426:1429	arg1	engineering					1438:1448	bone tissue engineering	1426:1448	bone tissue engineering	1426:1448	This study provides a simple and environmentally friendly technique to construct three-dimensional porous nHAp/CS composite scaffolds, which demonstrate promising potential by being a scaffold material for bone tissue engineering.
32204004	0	37	theme	nano-hydroxyapatite	49:67	arg1	colloid					69:75	a highly dispersed nano-hydroxyapatite colloid	30:75	a highly dispersed nano-hydroxyapatite colloid	30:75	Preparation and properties of a highly dispersed nano-hydroxyapatite colloid used as a reinforcing filler for chitosan.
32204004	6	38	theme	swelling	1046:1053	arg1	performance					1055:1065	excellent swelling performance	1036:1065	excellent swelling performance	1036:1065	The as-prepared nHAp/CS scaffolds exhibited a high porosity and excellent swelling performance.
32204004	5	39	theme	mechanical	873:882	arg1	properties					884:893	mechanical properties	873:893	mechanical properties	873:893	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds were thoroughly investigated.
32204004	0	40	used	used	77:80	arg2	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and properties of a highly dispersed nano-hydroxyapatite colloid used as a reinforcing filler for chitosan.
32204004	0	40	used	used	77:80	arg2	properties					16:25	properties	16:25	properties	16:25	Preparation and properties of a highly dispersed nano-hydroxyapatite colloid used as a reinforcing filler for chitosan.
32204004	3	41	theme	nHAp	507:510	arg1	solution					523:530	the homogenously dispersed nano-hydroxyapatite (nHAp) colloidal solution	459:530	the homogenously dispersed nano-hydroxyapatite (nHAp) colloidal solution	459:530	In this study, the homogenously dispersed nano-hydroxyapatite (nHAp) colloidal solution was first synthesized using a co-precipitation method.
32204004	0	42	theme	dispersed	39:47	arg1	colloid					69:75	a highly dispersed nano-hydroxyapatite colloid	30:75	a highly dispersed nano-hydroxyapatite colloid	30:75	Preparation and properties of a highly dispersed nano-hydroxyapatite colloid used as a reinforcing filler for chitosan.
32204004	6	43	theme	excellent	1036:1044	arg1	performance					1055:1065	excellent swelling performance	1036:1065	excellent swelling performance	1036:1065	The as-prepared nHAp/CS scaffolds exhibited a high porosity and excellent swelling performance.
32204004	7	44	theme	compressive	1156:1166	arg1	modulus					1168:1174	higher compressive modulus	1149:1174	higher compressive modulus	1149:1174	Compared with pure CS scaffolds, the nHAp/CS composite scaffolds not only showed higher compressive modulus but also exhibited better biocompatibility.
32204004	7	45	theme	nHAp/CS	1105:1111	arg1	scaffolds					1123:1131	the nHAp/CS composite scaffolds	1101:1131	the nHAp/CS composite scaffolds	1101:1131	Compared with pure CS scaffolds, the nHAp/CS composite scaffolds not only showed higher compressive modulus but also exhibited better biocompatibility.
32204004	1	46	theme	Hydroxyapatite/chitosan	120:142	arg1	composites					153:162	Hydroxyapatite/chitosan (HAp/CS) composites	120:162	Hydroxyapatite/chitosan (HAp/CS) composites	120:162	Hydroxyapatite/chitosan (HAp/CS) composites have been widely studied and applied in tissue engineering fields due to their excellent biocompatibility and degradability.
32204004	8	47	theme	simple	1242:1247	arg1	technique					1278:1286	a simple and environmentally friendly technique	1240:1286	a simple and environmentally friendly technique to construct three-dimensional porous nHAp/CS composite scaffolds, which demonstrate promising potential by being a scaffold material for bone tissue engineering	1240:1448	This study provides a simple and environmentally friendly technique to construct three-dimensional porous nHAp/CS composite scaffolds, which demonstrate promising potential by being a scaffold material for bone tissue engineering.
32204004	5	48	theme	phase	838:842	arg1	composition					844:854	phase composition	838:854	phase composition	838:854	The microstructure, porosity, phase composition, swelling ratio, mechanical properties, and biocompatibility of the nHAp/CS scaffolds were thoroughly investigated.
32204004	3	49	theme	nano-hydroxyapatite	486:504	arg1	solution					523:530	the homogenously dispersed nano-hydroxyapatite (nHAp) colloidal solution	459:530	the homogenously dispersed nano-hydroxyapatite (nHAp) colloidal solution	459:530	In this study, the homogenously dispersed nano-hydroxyapatite (nHAp) colloidal solution was first synthesized using a co-precipitation method.
32204004	4	50	theme	composite	655:663	arg1	scaffolds					665:673	The three-dimensional porous nano-hydroxyapatite/chitosan (nHAp/CS) composite scaffolds	587:673	The three-dimensional porous nano-hydroxyapatite/chitosan (nHAp/CS) composite scaffolds with different nHAp contents	587:702	The three-dimensional porous nano-hydroxyapatite/chitosan (nHAp/CS) composite scaffolds with different nHAp contents were then obtained through an environmentally friendly freeze-drying process without any cross-linking.
32204004	7	51	theme	composite	1113:1121	arg1	scaffolds					1123:1131	the nHAp/CS composite scaffolds	1101:1131	the nHAp/CS composite scaffolds	1101:1131	Compared with pure CS scaffolds, the nHAp/CS composite scaffolds not only showed higher compressive modulus but also exhibited better biocompatibility.
32204004	2	52	theme	CS	338:339	arg1	properties					324:333	the mechanical properties	309:333	the mechanical properties of CS	309:339	However, to improve the mechanical properties of CS, cross-linking agents are commonly added, which will seriously affect its biocompatibility and safety.
32204004	8	53	theme	environmentally	1253:1267	arg1	technique					1278:1286	a simple and environmentally friendly technique	1240:1286	a simple and environmentally friendly technique to construct three-dimensional porous nHAp/CS composite scaffolds, which demonstrate promising potential by being a scaffold material for bone tissue engineering	1240:1448	This study provides a simple and environmentally friendly technique to construct three-dimensional porous nHAp/CS composite scaffolds, which demonstrate promising potential by being a scaffold material for bone tissue engineering.
32204004	0	54	theme	colloid	69:75	arg1	properties					16:25	properties	16:25	properties	16:25	Preparation and properties of a highly dispersed nano-hydroxyapatite colloid used as a reinforcing filler for chitosan.
32204004	0	54	theme	colloid	69:75	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and properties of a highly dispersed nano-hydroxyapatite colloid used as a reinforcing filler for chitosan.
32204004	7	55	theme	CS	1087:1088	arg1	scaffolds					1090:1098	pure CS scaffolds	1082:1098	pure CS scaffolds	1082:1098	Compared with pure CS scaffolds, the nHAp/CS composite scaffolds not only showed higher compressive modulus but also exhibited better biocompatibility.
32204004	8	56	theme	composite	1334:1342	arg1	scaffolds					1344:1352	three-dimensional porous nHAp/CS composite scaffolds	1301:1352	three-dimensional porous nHAp/CS composite scaffolds	1301:1352	This study provides a simple and environmentally friendly technique to construct three-dimensional porous nHAp/CS composite scaffolds, which demonstrate promising potential by being a scaffold material for bone tissue engineering.
32204004	1	57	theme	excellent	243:251	arg1	biocompatibility					253:268	their excellent biocompatibility	237:268	their excellent biocompatibility	237:268	Hydroxyapatite/chitosan (HAp/CS) composites have been widely studied and applied in tissue engineering fields due to their excellent biocompatibility and degradability.
33862285	1	0	theme	lipids	152:157	arg1	form					144:147	the form	140:147	the form	140:147	Many microorganisms can accumulate biomass in the form of lipids and polysaccharides, which can be used for biofuels, bioplastics, food and feed.
33862285	7	1	theme	culturable	1232:1241	arg1	strain					1253:1258	every culturable microbial strain	1226:1258	every culturable microbial strain	1226:1258	The fattening factor described here should be easily estimatable in N-starvation for every culturable microbial strain, by measuring the amount of accumulated biomass.
33862285	6	2	from	variations	1067:1076	arg1	cycle					1086:1090	cell cycle	1081:1090	cell cycle	1081:1090	It is derived from considerations about the main metabolic aspects of cells' response to N-starvation, which induces variations in cell cycle, biomass production and biochemical composition.
33862285	6	2	from	variations	1067:1076	arg1	production					1101:1110	biomass production	1093:1110	biomass production	1093:1110	It is derived from considerations about the main metabolic aspects of cells' response to N-starvation, which induces variations in cell cycle, biomass production and biochemical composition.
33862285	6	2	from	variations	1067:1076	arg1	composition					1128:1138	biochemical composition	1116:1138	biochemical composition	1116:1138	It is derived from considerations about the main metabolic aspects of cells' response to N-starvation, which induces variations in cell cycle, biomass production and biochemical composition.
33862285	6	3	theme	metabolic	999:1007	arg1	aspects					1009:1015	the main metabolic aspects	990:1015	the main metabolic aspects of cells' response to N-starvation, which induces variations in cell cycle, biomass production and biochemical composition	990:1138	It is derived from considerations about the main metabolic aspects of cells' response to N-starvation, which induces variations in cell cycle, biomass production and biochemical composition.
33862285	1	4	used	used	193:196	arg2	form					144:147	the form	140:147	the form	140:147	Many microorganisms can accumulate biomass in the form of lipids and polysaccharides, which can be used for biofuels, bioplastics, food and feed.
33862285	4	5	theme	accumulation	771:782	arg1	ability					784:790	such strain-specific biomass accumulation ability	742:790	such strain-specific biomass accumulation ability	742:790	Here, a parameter termed "fattening factor" (ηx) is described to quantify such strain-specific biomass accumulation ability in bacteria, yeasts and microalgae.
33862285	4	6	theme	such	742:745	arg1	ability					784:790	such strain-specific biomass accumulation ability	742:790	such strain-specific biomass accumulation ability	742:790	Here, a parameter termed "fattening factor" (ηx) is described to quantify such strain-specific biomass accumulation ability in bacteria, yeasts and microalgae.
33862285	6	7	theme	main	994:997	arg1	aspects					1009:1015	the main metabolic aspects	990:1015	the main metabolic aspects of cells' response to N-starvation, which induces variations in cell cycle, biomass production and biochemical composition	990:1138	It is derived from considerations about the main metabolic aspects of cells' response to N-starvation, which induces variations in cell cycle, biomass production and biochemical composition.
33862285	4	8	theme	biomass	763:769	arg1	ability					784:790	such strain-specific biomass accumulation ability	742:790	such strain-specific biomass accumulation ability	742:790	Here, a parameter termed "fattening factor" (ηx) is described to quantify such strain-specific biomass accumulation ability in bacteria, yeasts and microalgae.
33862285	3	9	theme	biomass	557:563	arg1	ability					578:584	biomass accumulation ability	557:584	biomass accumulation ability	557:584	However, there is no general and easily comparable parameter available to compare biomass accumulation ability among different microbial strains, which could measure the competitive advantage.
33862285	6	10	theme	cell	1081:1084	arg1	cycle					1086:1090	cell cycle	1081:1090	cell cycle	1081:1090	It is derived from considerations about the main metabolic aspects of cells' response to N-starvation, which induces variations in cell cycle, biomass production and biochemical composition.
33862285	3	11	theme	available	536:544	arg1	parameter					526:534	no general and easily comparable parameter	493:534	no general and easily comparable parameter	493:534	However, there is no general and easily comparable parameter available to compare biomass accumulation ability among different microbial strains, which could measure the competitive advantage.
33862285	1	12	theme	polysaccharides	163:177	arg1	form					144:147	the form	140:147	the form	140:147	Many microorganisms can accumulate biomass in the form of lipids and polysaccharides, which can be used for biofuels, bioplastics, food and feed.
33862285	7	13	theme	fattening	1145:1153	arg1	factor					1155:1160	The fattening factor	1141:1160	The fattening factor described here	1141:1175	The fattening factor described here should be easily estimatable in N-starvation for every culturable microbial strain, by measuring the amount of accumulated biomass.
33862285	7	13	theme	fattening	1145:1153	arg1	estimatable					1194:1204	estimatable	1194:1204	estimatable	1194:1204	The fattening factor described here should be easily estimatable in N-starvation for every culturable microbial strain, by measuring the amount of accumulated biomass.
33862285	2	14	theme	innovative	245:254	arg1	bioprocesses					256:267	Some innovative bioprocesses	240:267	Some innovative bioprocesses	240:267	Some innovative bioprocesses exploit the competitive advantage provided by such accumulation ability, mainly under N-starvation, to select high-accumulating strains against biological contaminants, by using uncoupled nutrient feeding.
33862285	2	15	theme	high-accumulating	379:395	arg1	strains					397:403	high-accumulating strains	379:403	high-accumulating strains	379:403	Some innovative bioprocesses exploit the competitive advantage provided by such accumulation ability, mainly under N-starvation, to select high-accumulating strains against biological contaminants, by using uncoupled nutrient feeding.
33862285	0	16	theme	fattening	2:10	arg1	factor					12:17	A fattening factor	0:17	A fattening factor	0:17	A fattening factor to quantify the accumulation ability of microorganisms under N-starvation.
33862285	7	17	theme	microbial	1243:1251	arg1	strain					1253:1258	every culturable microbial strain	1226:1258	every culturable microbial strain	1226:1258	The fattening factor described here should be easily estimatable in N-starvation for every culturable microbial strain, by measuring the amount of accumulated biomass.
33862285	5	18	theme	accumulation	936:947	arg1	biomass					906:912	its biomass	902:912	its biomass	902:912	This parameter measures how many fold a microbial population can increase its biomass just as the result of accumulation.
33862285	5	18	theme	accumulation	936:947	arg1	result					926:931	the result	922:931	the result of accumulation	922:947	This parameter measures how many fold a microbial population can increase its biomass just as the result of accumulation.
33862285	2	19	theme	uncoupled	447:455	arg1	feeding					466:472	uncoupled nutrient feeding	447:472	uncoupled nutrient feeding	447:472	Some innovative bioprocesses exploit the competitive advantage provided by such accumulation ability, mainly under N-starvation, to select high-accumulating strains against biological contaminants, by using uncoupled nutrient feeding.
33862285	1	20	theme	Many	94:97	arg1	microorganisms					99:112	Many microorganisms	94:112	Many microorganisms	94:112	Many microorganisms can accumulate biomass in the form of lipids and polysaccharides, which can be used for biofuels, bioplastics, food and feed.
33862285	3	21	theme	accumulation	565:576	arg1	ability					578:584	biomass accumulation ability	557:584	biomass accumulation ability	557:584	However, there is no general and easily comparable parameter available to compare biomass accumulation ability among different microbial strains, which could measure the competitive advantage.
33862285	3	22	theme	different	592:600	arg1	strains					612:618	different microbial strains	592:618	different microbial strains	592:618	However, there is no general and easily comparable parameter available to compare biomass accumulation ability among different microbial strains, which could measure the competitive advantage.
33862285	7	23	from	estimatable	1194:1204	arg1	N-starvation					1209:1220	N-starvation	1209:1220	N-starvation for every culturable microbial strain	1209:1258	The fattening factor described here should be easily estimatable in N-starvation for every culturable microbial strain, by measuring the amount of accumulated biomass.
33862285	2	24	theme	competitive	281:291	arg1	advantage					293:301	the competitive advantage	277:301	the competitive advantage provided by such accumulation ability	277:339	Some innovative bioprocesses exploit the competitive advantage provided by such accumulation ability, mainly under N-starvation, to select high-accumulating strains against biological contaminants, by using uncoupled nutrient feeding.
33862285	3	25	dep	measure	633:639	arg1	could					627:631	could	627:631	could	627:631	However, there is no general and easily comparable parameter available to compare biomass accumulation ability among different microbial strains, which could measure the competitive advantage.
33862285	7	26	theme	biomass	1300:1306	arg1	amount					1278:1283	the amount	1274:1283	the amount of accumulated biomass	1274:1306	The fattening factor described here should be easily estimatable in N-starvation for every culturable microbial strain, by measuring the amount of accumulated biomass.
33862285	7	26	theme	biomass	1300:1306	arg1	biomass					1300:1306	accumulated biomass	1288:1306	accumulated biomass	1288:1306	The fattening factor described here should be easily estimatable in N-starvation for every culturable microbial strain, by measuring the amount of accumulated biomass.
33862285	5	27	theme	many	856:859	arg1	population					878:887	a microbial population	866:887	many fold a microbial population	856:887	This parameter measures how many fold a microbial population can increase its biomass just as the result of accumulation.
33862285	2	28	theme	biological	413:422	arg1	contaminants					424:435	biological contaminants	413:435	biological contaminants	413:435	Some innovative bioprocesses exploit the competitive advantage provided by such accumulation ability, mainly under N-starvation, to select high-accumulating strains against biological contaminants, by using uncoupled nutrient feeding.
33862285	4	29	theme	factor	704:709	arg1	"					710:710	fattening factor"	694:710	fattening factor" (ηx)	694:715	Here, a parameter termed "fattening factor" (ηx) is described to quantify such strain-specific biomass accumulation ability in bacteria, yeasts and microalgae.
33862285	4	29	theme	factor	704:709	arg1	ηx					713:714	ηx	713:714	ηx	713:714	Here, a parameter termed "fattening factor" (ηx) is described to quantify such strain-specific biomass accumulation ability in bacteria, yeasts and microalgae.
33862285	7	30	from	N-starvation	1209:1220	arg1	factor					1155:1160	The fattening factor	1141:1160	The fattening factor described here	1141:1175	The fattening factor described here should be easily estimatable in N-starvation for every culturable microbial strain, by measuring the amount of accumulated biomass.
33862285	7	30	from	N-starvation	1209:1220	arg1	estimatable					1194:1204	estimatable	1194:1204	estimatable	1194:1204	The fattening factor described here should be easily estimatable in N-starvation for every culturable microbial strain, by measuring the amount of accumulated biomass.
33862285	3	31	theme	microbial	602:610	arg1	strains					612:618	different microbial strains	592:618	different microbial strains	592:618	However, there is no general and easily comparable parameter available to compare biomass accumulation ability among different microbial strains, which could measure the competitive advantage.
33862285	7	32	theme	accumulated	1288:1298	arg1	biomass					1300:1306	accumulated biomass	1288:1306	accumulated biomass	1288:1306	The fattening factor described here should be easily estimatable in N-starvation for every culturable microbial strain, by measuring the amount of accumulated biomass.
33862285	0	33	theme	accumulation	35:46	arg1	ability					48:54	the accumulation ability	31:54	the accumulation ability of microorganisms	31:72	A fattening factor to quantify the accumulation ability of microorganisms under N-starvation.
33862285	3	34	theme	general	496:502	arg1	parameter					526:534	no general and easily comparable parameter	493:534	no general and easily comparable parameter	493:534	However, there is no general and easily comparable parameter available to compare biomass accumulation ability among different microbial strains, which could measure the competitive advantage.
33862285	4	35	theme	fattening	694:702	arg1	"					710:710	fattening factor"	694:710	fattening factor" (ηx)	694:715	Here, a parameter termed "fattening factor" (ηx) is described to quantify such strain-specific biomass accumulation ability in bacteria, yeasts and microalgae.
33862285	4	35	theme	fattening	694:702	arg1	ηx					713:714	ηx	713:714	ηx	713:714	Here, a parameter termed "fattening factor" (ηx) is described to quantify such strain-specific biomass accumulation ability in bacteria, yeasts and microalgae.
33862285	2	36	theme	accumulation	320:331	arg1	ability					333:339	such accumulation ability	315:339	such accumulation ability	315:339	Some innovative bioprocesses exploit the competitive advantage provided by such accumulation ability, mainly under N-starvation, to select high-accumulating strains against biological contaminants, by using uncoupled nutrient feeding.
33862285	4	37	theme	strain-specific	747:761	arg1	ability					784:790	such strain-specific biomass accumulation ability	742:790	such strain-specific biomass accumulation ability	742:790	Here, a parameter termed "fattening factor" (ηx) is described to quantify such strain-specific biomass accumulation ability in bacteria, yeasts and microalgae.
33862285	6	38	theme	response	1027:1034	arg1	aspects					1009:1015	the main metabolic aspects	990:1015	the main metabolic aspects of cells' response to N-starvation, which induces variations in cell cycle, biomass production and biochemical composition	990:1138	It is derived from considerations about the main metabolic aspects of cells' response to N-starvation, which induces variations in cell cycle, biomass production and biochemical composition.
33862285	3	39	theme	competitive	645:655	arg1	advantage					657:665	the competitive advantage	641:665	the competitive advantage	641:665	However, there is no general and easily comparable parameter available to compare biomass accumulation ability among different microbial strains, which could measure the competitive advantage.
33862285	2	40	theme	such	315:318	arg1	ability					333:339	such accumulation ability	315:339	such accumulation ability	315:339	Some innovative bioprocesses exploit the competitive advantage provided by such accumulation ability, mainly under N-starvation, to select high-accumulating strains against biological contaminants, by using uncoupled nutrient feeding.
33862285	6	41	theme	biochemical	1116:1126	arg1	composition					1128:1138	biochemical composition	1116:1138	biochemical composition	1116:1138	It is derived from considerations about the main metabolic aspects of cells' response to N-starvation, which induces variations in cell cycle, biomass production and biochemical composition.
33862285	0	42	theme	microorganisms	59:72	arg1	ability					48:54	the accumulation ability	31:54	the accumulation ability of microorganisms	31:72	A fattening factor to quantify the accumulation ability of microorganisms under N-starvation.
33862285	6	43	theme	biomass	1093:1099	arg1	production					1101:1110	biomass production	1093:1110	biomass production	1093:1110	It is derived from considerations about the main metabolic aspects of cells' response to N-starvation, which induces variations in cell cycle, biomass production and biochemical composition.
33862285	6	44	attach	derived	956:962	arg1	considerations					969:982	considerations	969:982	considerations	969:982	It is derived from considerations about the main metabolic aspects of cells' response to N-starvation, which induces variations in cell cycle, biomass production and biochemical composition.
33862285	6	44	attach	derived	956:962	arg2	It					950:951	It	950:951	It	950:951	It is derived from considerations about the main metabolic aspects of cells' response to N-starvation, which induces variations in cell cycle, biomass production and biochemical composition.
33862285	3	45	theme	comparable	515:524	arg1	parameter					526:534	no general and easily comparable parameter	493:534	no general and easily comparable parameter	493:534	However, there is no general and easily comparable parameter available to compare biomass accumulation ability among different microbial strains, which could measure the competitive advantage.
33862285	5	46	theme	microbial	868:876	arg1	population					878:887	a microbial population	866:887	many fold a microbial population	856:887	This parameter measures how many fold a microbial population can increase its biomass just as the result of accumulation.
33862285	2	47	theme	nutrient	457:464	arg1	feeding					466:472	uncoupled nutrient feeding	447:472	uncoupled nutrient feeding	447:472	Some innovative bioprocesses exploit the competitive advantage provided by such accumulation ability, mainly under N-starvation, to select high-accumulating strains against biological contaminants, by using uncoupled nutrient feeding.
34197993	3	0	with	bromine	531:537	arg1	topography					570:579	lotus leaf-like topography	554:579	lotus leaf-like topography	554:579	By combining multicomponent thermo-curing and replica molding, a polydimethylsiloxane surface containing bromine (PDMS-Br) with lotus leaf-like topography is obtained.
34197993	2	1	theme	polymers	336:343	arg1	effects					312:318	the effects	308:318	the effects of heparin-like polymers and lotus leaf-like topography on surface vascular cell behavior	308:408	In this study, the effects of heparin-like polymers and lotus leaf-like topography on surface vascular cell behavior are considered.
34197993	1	2	theme	chemical	237:244	arg1	composition					246:256	surface chemical composition	229:256	surface chemical composition	229:256	Vascular cell behavior on material surfaces, such as heparin-like polymers, can be affected by the surface chemical composition and surface topological structure.
34197993	6	3	theme	topography	978:987	arg1	introduction					946:957	The introduction	942:957	The introduction of lotus leaf-like topography	942:987	The introduction of lotus leaf-like topography results in different degrees of decrease in cell density on different heparin-like polymer-modified surfaces.
34197993	4	4	theme	different	621:629	arg1	compositions					640:651	different chemical compositions	621:651	different chemical compositions	621:651	Heparin-like polymers with different chemical compositions are grafted onto PDMS-Br surfaces using visible-light-induced graft polymerization.
34197993	7	5	theme	protein	1209:1215	arg1	adsorption					1217:1226	protein adsorption	1209:1226	protein adsorption	1209:1226	In addition, the combination of heparin-like polymers and lotus leaf-like topography results in the change in protein adsorption, indicating that the two factors may affect the surface vascular cell behavior by affecting the adsorption of relative proteins.
34197993	3	6	theme	leaf-like	560:568	arg1	topography					570:579	lotus leaf-like topography	554:579	lotus leaf-like topography	554:579	By combining multicomponent thermo-curing and replica molding, a polydimethylsiloxane surface containing bromine (PDMS-Br) with lotus leaf-like topography is obtained.
34197993	6	7	theme	lotus	962:966	arg1	topography					978:987	lotus leaf-like topography	962:987	lotus leaf-like topography	962:987	The introduction of lotus leaf-like topography results in different degrees of decrease in cell density on different heparin-like polymer-modified surfaces.
34197993	4	8	theme	graft	715:719	arg1	polymerization					721:734	visible-light-induced graft polymerization	693:734	visible-light-induced graft polymerization	693:734	Heparin-like polymers with different chemical compositions are grafted onto PDMS-Br surfaces using visible-light-induced graft polymerization.
34197993	4	9	theme	PDMS-Br	670:676	arg1	surfaces					678:685	PDMS-Br surfaces	670:685	PDMS-Br surfaces using visible-light-induced graft polymerization	670:734	Heparin-like polymers with different chemical compositions are grafted onto PDMS-Br surfaces using visible-light-induced graft polymerization.
34197993	7	10	theme	topography	1173:1182	arg1	combination					1116:1126	the combination	1112:1126	the combination of heparin-like polymers and lotus leaf-like topography	1112:1182	In addition, the combination of heparin-like polymers and lotus leaf-like topography results in the change in protein adsorption, indicating that the two factors may affect the surface vascular cell behavior by affecting the adsorption of relative proteins.
34197993	6	11	theme	cell	1033:1036	arg1	density					1038:1044	cell density	1033:1044	cell density on different heparin-like polymer-modified surfaces	1033:1096	The introduction of lotus leaf-like topography results in different degrees of decrease in cell density on different heparin-like polymer-modified surfaces.
34197993	8	12	theme	bionic	1376:1381	arg1	topography					1391:1400	bionic surface topography	1376:1400	bionic surface topography	1376:1400	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces suggests a new way of engineering cell-material interactions.
34197993	7	13	theme	lotus	1157:1161	arg1	topography					1173:1182	lotus leaf-like topography	1157:1182	lotus leaf-like topography	1157:1182	In addition, the combination of heparin-like polymers and lotus leaf-like topography results in the change in protein adsorption, indicating that the two factors may affect the surface vascular cell behavior by affecting the adsorption of relative proteins.
34197993	6	14	theme	decrease	1021:1028	arg1	degrees					1010:1016	different degrees	1000:1016	different degrees of decrease in cell density on different heparin-like polymer-modified surfaces	1000:1096	The introduction of lotus leaf-like topography results in different degrees of decrease in cell density on different heparin-like polymer-modified surfaces.
34197993	8	15	theme	topography	1391:1400	arg1	combination					1361:1371	The combination	1357:1371	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces	1357:1480	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces suggests a new way of engineering cell-material interactions.
34197993	7	16	theme	polymers	1144:1151	arg1	combination					1116:1126	the combination	1112:1126	the combination of heparin-like polymers and lotus leaf-like topography	1112:1182	In addition, the combination of heparin-like polymers and lotus leaf-like topography results in the change in protein adsorption, indicating that the two factors may affect the surface vascular cell behavior by affecting the adsorption of relative proteins.
34197993	8	17	theme	different	1406:1414	arg1	components					1425:1434	different chemical components	1406:1434	different chemical components of heparin-like polymers on material surfaces	1406:1480	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces suggests a new way of engineering cell-material interactions.
34197993	1	18	theme	Vascular	130:137	arg1	behavior					144:151	Vascular cell behavior	130:151	Vascular cell behavior on material surfaces, such as heparin-like polymers,	130:204	Vascular cell behavior on material surfaces, such as heparin-like polymers, can be affected by the surface chemical composition and surface topological structure.
34197993	8	19	theme	components	1425:1434	arg1	combination					1361:1371	The combination	1357:1371	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces	1357:1480	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces suggests a new way of engineering cell-material interactions.
34197993	0	20	from	behavior	14:21	arg1	polymers					39:46	heparin-like polymers	26:46	heparin-like polymers	26:46	Vascular cell behavior on heparin-like polymers modified silicone surfaces: The prominent role of the lotus leaf-like topography.
34197993	8	21	theme	heparin-like	1439:1450	arg1	polymers					1452:1459	heparin-like polymers	1439:1459	heparin-like polymers on material surfaces	1439:1480	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces suggests a new way of engineering cell-material interactions.
34197993	1	22	theme	material	156:163	arg1	polymers					196:203	heparin-like polymers	183:203	heparin-like polymers	183:203	Vascular cell behavior on material surfaces, such as heparin-like polymers, can be affected by the surface chemical composition and surface topological structure.
34197993	1	22	theme	material	156:163	arg1	surfaces					165:172	material surfaces	156:172	material surfaces	156:172	Vascular cell behavior on material surfaces, such as heparin-like polymers, can be affected by the surface chemical composition and surface topological structure.
34197993	8	23	from	topography	1391:1400	arg1	surfaces					1473:1480	material surfaces	1464:1480	material surfaces	1464:1480	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces suggests a new way of engineering cell-material interactions.
34197993	6	24	theme	heparin-like	1059:1070	arg1	surfaces					1089:1096	different heparin-like polymer-modified surfaces	1049:1096	different heparin-like polymer-modified surfaces	1049:1096	The introduction of lotus leaf-like topography results in different degrees of decrease in cell density on different heparin-like polymer-modified surfaces.
34197993	2	25	theme	vascular	387:394	arg1	behavior					401:408	surface vascular cell behavior	379:408	surface vascular cell behavior	379:408	In this study, the effects of heparin-like polymers and lotus leaf-like topography on surface vascular cell behavior are considered.
34197993	7	26	theme	vascular	1284:1291	arg1	behavior					1298:1305	the surface vascular cell behavior	1272:1305	the surface vascular cell behavior	1272:1305	In addition, the combination of heparin-like polymers and lotus leaf-like topography results in the change in protein adsorption, indicating that the two factors may affect the surface vascular cell behavior by affecting the adsorption of relative proteins.
34197993	6	27	theme	different	1000:1008	arg1	degrees					1010:1016	different degrees	1000:1016	different degrees of decrease in cell density on different heparin-like polymer-modified surfaces	1000:1096	The introduction of lotus leaf-like topography results in different degrees of decrease in cell density on different heparin-like polymer-modified surfaces.
34197993	0	28	theme	Vascular	0:7	arg1	behavior					14:21	Vascular cell behavior	0:21	Vascular cell behavior on heparin-like polymers	0:46	Vascular cell behavior on heparin-like polymers modified silicone surfaces: The prominent role of the lotus leaf-like topography.
34197993	1	29	theme	surface	262:268	arg1	structure					282:290	surface topological structure	262:290	surface topological structure	262:290	Vascular cell behavior on material surfaces, such as heparin-like polymers, can be affected by the surface chemical composition and surface topological structure.
34197993	8	30	from	components	1425:1434	arg1	surfaces					1473:1480	material surfaces	1464:1480	material surfaces	1464:1480	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces suggests a new way of engineering cell-material interactions.
34197993	6	31	from	density	1038:1044	arg1	surfaces					1089:1096	different heparin-like polymer-modified surfaces	1049:1096	different heparin-like polymer-modified surfaces	1049:1096	The introduction of lotus leaf-like topography results in different degrees of decrease in cell density on different heparin-like polymer-modified surfaces.
34197993	6	31	from	density	1038:1044	arg1	degrees					1010:1016	different degrees	1000:1016	different degrees of decrease in cell density on different heparin-like polymer-modified surfaces	1000:1096	The introduction of lotus leaf-like topography results in different degrees of decrease in cell density on different heparin-like polymer-modified surfaces.
34197993	3	32	theme	replica	472:478	arg1	molding					480:486	replica molding	472:486	replica molding	472:486	By combining multicomponent thermo-curing and replica molding, a polydimethylsiloxane surface containing bromine (PDMS-Br) with lotus leaf-like topography is obtained.
34197993	0	33	dep	surfaces	66:73	arg1	role					90:93	The prominent role	76:93	silicone surfaces: The prominent role of the lotus leaf-like topography	57:127	Vascular cell behavior on heparin-like polymers modified silicone surfaces: The prominent role of the lotus leaf-like topography.
34197993	7	34	from	change	1199:1204	arg1	adsorption					1217:1226	protein adsorption	1209:1226	protein adsorption	1209:1226	In addition, the combination of heparin-like polymers and lotus leaf-like topography results in the change in protein adsorption, indicating that the two factors may affect the surface vascular cell behavior by affecting the adsorption of relative proteins.
34197993	8	35	theme	engineering	1504:1514	arg1	interactions					1530:1541	engineering cell-material interactions	1504:1541	engineering cell-material interactions	1504:1541	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces suggests a new way of engineering cell-material interactions.
34197993	0	36	theme	heparin-like	26:37	arg1	polymers					39:46	heparin-like polymers	26:46	heparin-like polymers	26:46	Vascular cell behavior on heparin-like polymers modified silicone surfaces: The prominent role of the lotus leaf-like topography.
34197993	8	37	theme	interactions	1530:1541	arg1	way					1497:1499	a new way	1491:1499	a new way of engineering cell-material interactions	1491:1541	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces suggests a new way of engineering cell-material interactions.
34197993	3	38	theme	polydimethylsiloxane	491:510	arg1	bromine					531:537	a polydimethylsiloxane surface containing bromine	489:537	a polydimethylsiloxane surface containing bromine (PDMS-Br) with lotus leaf-like topography	489:579	By combining multicomponent thermo-curing and replica molding, a polydimethylsiloxane surface containing bromine (PDMS-Br) with lotus leaf-like topography is obtained.
34197993	3	38	theme	polydimethylsiloxane	491:510	arg1	PDMS-Br					540:546	PDMS-Br	540:546	PDMS-Br	540:546	By combining multicomponent thermo-curing and replica molding, a polydimethylsiloxane surface containing bromine (PDMS-Br) with lotus leaf-like topography is obtained.
34197993	4	39	theme	chemical	631:638	arg1	compositions					640:651	different chemical compositions	621:651	different chemical compositions	621:651	Heparin-like polymers with different chemical compositions are grafted onto PDMS-Br surfaces using visible-light-induced graft polymerization.
34197993	2	40	theme	leaf-like	355:363	arg1	topography					365:374	lotus leaf-like topography	349:374	lotus leaf-like topography	349:374	In this study, the effects of heparin-like polymers and lotus leaf-like topography on surface vascular cell behavior are considered.
34197993	8	41	from	polymers	1452:1459	arg1	surfaces					1473:1480	material surfaces	1464:1480	material surfaces	1464:1480	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces suggests a new way of engineering cell-material interactions.
34197993	7	42	theme	surface	1276:1282	arg1	behavior					1298:1305	the surface vascular cell behavior	1272:1305	the surface vascular cell behavior	1272:1305	In addition, the combination of heparin-like polymers and lotus leaf-like topography results in the change in protein adsorption, indicating that the two factors may affect the surface vascular cell behavior by affecting the adsorption of relative proteins.
34197993	5	43	theme	vascular	843:850	arg1	cells					852:856	vascular cells	843:856	vascular cells	843:856	Compared with unmodified PDMS-Br, surfaces modified by sulfonate-containing polymers are more friendly to vascular cells, while those modified by a glyco-polymer are much more resistant to vascular cells.
34197993	3	44	theme	containing	520:529	arg1	bromine					531:537	a polydimethylsiloxane surface containing bromine	489:537	a polydimethylsiloxane surface containing bromine (PDMS-Br) with lotus leaf-like topography	489:579	By combining multicomponent thermo-curing and replica molding, a polydimethylsiloxane surface containing bromine (PDMS-Br) with lotus leaf-like topography is obtained.
34197993	3	44	theme	containing	520:529	arg1	PDMS-Br					540:546	PDMS-Br	540:546	PDMS-Br	540:546	By combining multicomponent thermo-curing and replica molding, a polydimethylsiloxane surface containing bromine (PDMS-Br) with lotus leaf-like topography is obtained.
34197993	1	45	theme	surface	229:235	arg1	composition					246:256	surface chemical composition	229:256	surface chemical composition	229:256	Vascular cell behavior on material surfaces, such as heparin-like polymers, can be affected by the surface chemical composition and surface topological structure.
34197993	2	46	from	effects	312:318	arg1	behavior					401:408	surface vascular cell behavior	379:408	surface vascular cell behavior	379:408	In this study, the effects of heparin-like polymers and lotus leaf-like topography on surface vascular cell behavior are considered.
34197993	7	47	theme	relative	1338:1345	arg1	proteins					1347:1354	relative proteins	1338:1354	relative proteins	1338:1354	In addition, the combination of heparin-like polymers and lotus leaf-like topography results in the change in protein adsorption, indicating that the two factors may affect the surface vascular cell behavior by affecting the adsorption of relative proteins.
34197993	4	48	theme	Heparin-like	594:605	arg1	polymers					607:614	Heparin-like polymers	594:614	Heparin-like polymers with different chemical compositions	594:651	Heparin-like polymers with different chemical compositions are grafted onto PDMS-Br surfaces using visible-light-induced graft polymerization.
34197993	3	49	theme	lotus	554:558	arg1	topography					570:579	lotus leaf-like topography	554:579	lotus leaf-like topography	554:579	By combining multicomponent thermo-curing and replica molding, a polydimethylsiloxane surface containing bromine (PDMS-Br) with lotus leaf-like topography is obtained.
34197993	6	50	theme	leaf-like	968:976	arg1	topography					978:987	lotus leaf-like topography	962:987	lotus leaf-like topography	962:987	The introduction of lotus leaf-like topography results in different degrees of decrease in cell density on different heparin-like polymer-modified surfaces.
34197993	2	51	theme	heparin-like	323:334	arg1	polymers					336:343	heparin-like polymers	323:343	heparin-like polymers	323:343	In this study, the effects of heparin-like polymers and lotus leaf-like topography on surface vascular cell behavior are considered.
34197993	5	52	theme	vascular	926:933	arg1	cells					935:939	vascular cells	926:939	vascular cells	926:939	Compared with unmodified PDMS-Br, surfaces modified by sulfonate-containing polymers are more friendly to vascular cells, while those modified by a glyco-polymer are much more resistant to vascular cells.
34197993	4	53	theme	visible-light-induced	693:713	arg1	polymerization					721:734	visible-light-induced graft polymerization	693:734	visible-light-induced graft polymerization	693:734	Heparin-like polymers with different chemical compositions are grafted onto PDMS-Br surfaces using visible-light-induced graft polymerization.
34197993	6	54	theme	different	1049:1057	arg1	surfaces					1089:1096	different heparin-like polymer-modified surfaces	1049:1096	different heparin-like polymer-modified surfaces	1049:1096	The introduction of lotus leaf-like topography results in different degrees of decrease in cell density on different heparin-like polymer-modified surfaces.
34197993	7	55	theme	leaf-like	1163:1171	arg1	topography					1173:1182	lotus leaf-like topography	1157:1182	lotus leaf-like topography	1157:1182	In addition, the combination of heparin-like polymers and lotus leaf-like topography results in the change in protein adsorption, indicating that the two factors may affect the surface vascular cell behavior by affecting the adsorption of relative proteins.
34197993	1	56	theme	heparin-like	183:194	arg1	polymers					196:203	heparin-like polymers	183:203	heparin-like polymers	183:203	Vascular cell behavior on material surfaces, such as heparin-like polymers, can be affected by the surface chemical composition and surface topological structure.
34197993	7	57	theme	cell	1293:1296	arg1	behavior					1298:1305	the surface vascular cell behavior	1272:1305	the surface vascular cell behavior	1272:1305	In addition, the combination of heparin-like polymers and lotus leaf-like topography results in the change in protein adsorption, indicating that the two factors may affect the surface vascular cell behavior by affecting the adsorption of relative proteins.
34197993	7	58	theme	heparin-like	1131:1142	arg1	polymers					1144:1151	heparin-like polymers	1131:1151	heparin-like polymers	1131:1151	In addition, the combination of heparin-like polymers and lotus leaf-like topography results in the change in protein adsorption, indicating that the two factors may affect the surface vascular cell behavior by affecting the adsorption of relative proteins.
34197993	0	59	theme	topography	118:127	arg1	role					90:93	The prominent role	76:93	silicone surfaces: The prominent role of the lotus leaf-like topography	57:127	Vascular cell behavior on heparin-like polymers modified silicone surfaces: The prominent role of the lotus leaf-like topography.
34197993	1	60	dep	composition	246:256	arg1	the					225:227	the	225:227	the	225:227	Vascular cell behavior on material surfaces, such as heparin-like polymers, can be affected by the surface chemical composition and surface topological structure.
34197993	8	61	theme	surface	1383:1389	arg1	topography					1391:1400	bionic surface topography	1376:1400	bionic surface topography	1376:1400	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces suggests a new way of engineering cell-material interactions.
34197993	5	62	theme	unmodified	751:760	arg1	PDMS-Br					762:768	unmodified PDMS-Br	751:768	unmodified PDMS-Br	751:768	Compared with unmodified PDMS-Br, surfaces modified by sulfonate-containing polymers are more friendly to vascular cells, while those modified by a glyco-polymer are much more resistant to vascular cells.
34197993	4	63	with	polymers	607:614	arg1	compositions					640:651	different chemical compositions	621:651	different chemical compositions	621:651	Heparin-like polymers with different chemical compositions are grafted onto PDMS-Br surfaces using visible-light-induced graft polymerization.
34197993	1	64	theme	cell	139:142	arg1	behavior					144:151	Vascular cell behavior	130:151	Vascular cell behavior on material surfaces, such as heparin-like polymers,	130:204	Vascular cell behavior on material surfaces, such as heparin-like polymers, can be affected by the surface chemical composition and surface topological structure.
34197993	8	65	theme	chemical	1416:1423	arg1	components					1425:1434	different chemical components	1406:1434	different chemical components of heparin-like polymers on material surfaces	1406:1480	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces suggests a new way of engineering cell-material interactions.
34197993	0	66	theme	prominent	80:88	arg1	role					90:93	The prominent role	76:93	silicone surfaces: The prominent role of the lotus leaf-like topography	57:127	Vascular cell behavior on heparin-like polymers modified silicone surfaces: The prominent role of the lotus leaf-like topography.
34197993	0	67	theme	leaf-like	108:116	arg1	topography					118:127	the lotus leaf-like topography	98:127	the lotus leaf-like topography	98:127	Vascular cell behavior on heparin-like polymers modified silicone surfaces: The prominent role of the lotus leaf-like topography.
34197993	5	68	theme	sulfonate-containing	792:811	arg1	polymers					813:820	sulfonate-containing polymers	792:820	sulfonate-containing polymers	792:820	Compared with unmodified PDMS-Br, surfaces modified by sulfonate-containing polymers are more friendly to vascular cells, while those modified by a glyco-polymer are much more resistant to vascular cells.
34197993	6	69	theme	polymer-modified	1072:1087	arg1	surfaces					1089:1096	different heparin-like polymer-modified surfaces	1049:1096	different heparin-like polymer-modified surfaces	1049:1096	The introduction of lotus leaf-like topography results in different degrees of decrease in cell density on different heparin-like polymer-modified surfaces.
34197993	8	70	theme	polymers	1452:1459	arg1	components					1425:1434	different chemical components	1406:1434	different chemical components of heparin-like polymers on material surfaces	1406:1480	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces suggests a new way of engineering cell-material interactions.
34197993	8	70	theme	polymers	1452:1459	arg1	topography					1391:1400	bionic surface topography	1376:1400	bionic surface topography	1376:1400	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces suggests a new way of engineering cell-material interactions.
34197993	0	71	theme	lotus	102:106	arg1	topography					118:127	the lotus leaf-like topography	98:127	the lotus leaf-like topography	98:127	Vascular cell behavior on heparin-like polymers modified silicone surfaces: The prominent role of the lotus leaf-like topography.
34197993	1	72	from	behavior	144:151	arg1	polymers					196:203	heparin-like polymers	183:203	heparin-like polymers	183:203	Vascular cell behavior on material surfaces, such as heparin-like polymers, can be affected by the surface chemical composition and surface topological structure.
34197993	1	72	from	behavior	144:151	arg1	surfaces					165:172	material surfaces	156:172	material surfaces	156:172	Vascular cell behavior on material surfaces, such as heparin-like polymers, can be affected by the surface chemical composition and surface topological structure.
34197993	8	73	theme	material	1464:1471	arg1	surfaces					1473:1480	material surfaces	1464:1480	material surfaces	1464:1480	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces suggests a new way of engineering cell-material interactions.
34197993	2	74	theme	surface	379:385	arg1	behavior					401:408	surface vascular cell behavior	379:408	surface vascular cell behavior	379:408	In this study, the effects of heparin-like polymers and lotus leaf-like topography on surface vascular cell behavior are considered.
34197993	0	75	theme	cell	9:12	arg1	behavior					14:21	Vascular cell behavior	0:21	Vascular cell behavior on heparin-like polymers	0:46	Vascular cell behavior on heparin-like polymers modified silicone surfaces: The prominent role of the lotus leaf-like topography.
34197993	6	76	from	surfaces	1089:1096	arg1	decrease					1021:1028	decrease	1021:1028	decrease in cell density on different heparin-like polymer-modified surfaces	1021:1096	The introduction of lotus leaf-like topography results in different degrees of decrease in cell density on different heparin-like polymer-modified surfaces.
34197993	2	77	theme	topography	365:374	arg1	effects					312:318	the effects	308:318	the effects of heparin-like polymers and lotus leaf-like topography on surface vascular cell behavior	308:408	In this study, the effects of heparin-like polymers and lotus leaf-like topography on surface vascular cell behavior are considered.
34197993	1	78	theme	topological	270:280	arg1	structure					282:290	surface topological structure	262:290	surface topological structure	262:290	Vascular cell behavior on material surfaces, such as heparin-like polymers, can be affected by the surface chemical composition and surface topological structure.
34197993	8	79	theme	new	1493:1495	arg1	way					1497:1499	a new way	1491:1499	a new way of engineering cell-material interactions	1491:1541	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces suggests a new way of engineering cell-material interactions.
34197993	2	80	theme	cell	396:399	arg1	behavior					401:408	surface vascular cell behavior	379:408	surface vascular cell behavior	379:408	In this study, the effects of heparin-like polymers and lotus leaf-like topography on surface vascular cell behavior are considered.
34197993	0	81	theme	silicone	57:64	arg1	surfaces					66:73	silicone surfaces	57:73	silicone surfaces: The prominent role of the lotus leaf-like topography	57:127	Vascular cell behavior on heparin-like polymers modified silicone surfaces: The prominent role of the lotus leaf-like topography.
34197993	6	82	from	degrees	1010:1016	arg1	density					1038:1044	cell density	1033:1044	cell density on different heparin-like polymer-modified surfaces	1033:1096	The introduction of lotus leaf-like topography results in different degrees of decrease in cell density on different heparin-like polymer-modified surfaces.
34197993	8	83	theme	cell-material	1516:1528	arg1	interactions					1530:1541	engineering cell-material interactions	1504:1541	engineering cell-material interactions	1504:1541	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces suggests a new way of engineering cell-material interactions.
34197993	3	84	theme	surface	512:518	arg1	bromine					531:537	a polydimethylsiloxane surface containing bromine	489:537	a polydimethylsiloxane surface containing bromine (PDMS-Br) with lotus leaf-like topography	489:579	By combining multicomponent thermo-curing and replica molding, a polydimethylsiloxane surface containing bromine (PDMS-Br) with lotus leaf-like topography is obtained.
34197993	3	84	theme	surface	512:518	arg1	PDMS-Br					540:546	PDMS-Br	540:546	PDMS-Br	540:546	By combining multicomponent thermo-curing and replica molding, a polydimethylsiloxane surface containing bromine (PDMS-Br) with lotus leaf-like topography is obtained.
34197993	8	85	from	surfaces	1473:1480	arg1	components					1425:1434	different chemical components	1406:1434	different chemical components of heparin-like polymers on material surfaces	1406:1480	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces suggests a new way of engineering cell-material interactions.
34197993	8	85	from	surfaces	1473:1480	arg1	topography					1391:1400	bionic surface topography	1376:1400	bionic surface topography	1376:1400	The combination of bionic surface topography and different chemical components of heparin-like polymers on material surfaces suggests a new way of engineering cell-material interactions.
34197993	6	86	from	decrease	1021:1028	arg1	surfaces					1089:1096	different heparin-like polymer-modified surfaces	1049:1096	different heparin-like polymer-modified surfaces	1049:1096	The introduction of lotus leaf-like topography results in different degrees of decrease in cell density on different heparin-like polymer-modified surfaces.
34197993	6	86	from	decrease	1021:1028	arg1	density					1038:1044	cell density	1033:1044	cell density on different heparin-like polymer-modified surfaces	1033:1096	The introduction of lotus leaf-like topography results in different degrees of decrease in cell density on different heparin-like polymer-modified surfaces.
34197993	7	87	theme	proteins	1347:1354	arg1	adsorption					1324:1333	the adsorption	1320:1333	the adsorption of relative proteins	1320:1354	In addition, the combination of heparin-like polymers and lotus leaf-like topography results in the change in protein adsorption, indicating that the two factors may affect the surface vascular cell behavior by affecting the adsorption of relative proteins.
34197993	3	88	theme	multicomponent	439:452	arg1	thermo-curing					454:466	multicomponent thermo-curing	439:466	multicomponent thermo-curing	439:466	By combining multicomponent thermo-curing and replica molding, a polydimethylsiloxane surface containing bromine (PDMS-Br) with lotus leaf-like topography is obtained.
34197993	2	89	theme	lotus	349:353	arg1	topography					365:374	lotus leaf-like topography	349:374	lotus leaf-like topography	349:374	In this study, the effects of heparin-like polymers and lotus leaf-like topography on surface vascular cell behavior are considered.
33990049	6	0	theme	metal	1349:1353	arg1	ions					1355:1358	metal ions	1349:1358	metal ions	1349:1358	This study successfully demonstrated an innovative method to fabricate biosorbents from abundant and renewable natural polymers (cellulose and chitosan) for removing metal ions from water.
33990049	4	1	with	hydrogel	731:738	arg1	chitosan					767:774	63% chitosan	763:774	63% chitosan	763:774	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
33990049	4	1	with	hydrogel	731:738	arg1	cellulose					749:757	37% cellulose	745:757	37% cellulose	745:757	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
33990049	2	2	theme	%	405:405	arg1	solution					415:422	a 60 wt% aqueous solution	398:422	a 60 wt% aqueous solution of LiBr	398:430	In this study, cellulose-chitosan composite hydrogels were fabricated via a co-dissolution and regeneration process using a molten salt hydrate (a 60 wt% aqueous solution of LiBr) as a solvent.
33990049	5	3	theme	Cu2+ > Zn2+ > Co2+	1163:1180	arg1	order					1154:1158	the order	1150:1158	the order of Cu2+ > Zn2+ > Co2+	1150:1180	Competitive adsorption from a mixed metals solution revealed that the cellulose-chitosan composite hydrogel exhibited selective adsorption of the metals in the order of Cu2+ > Zn2+ > Co2+.
33990049	4	4	theme	metal	876:880	arg1	concentration					882:894	initial metal concentration	868:894	initial metal concentration	868:894	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
33990049	2	5	theme	60 wt	400:404	arg1	%					405:405	%	405:405	%	405:405	In this study, cellulose-chitosan composite hydrogels were fabricated via a co-dissolution and regeneration process using a molten salt hydrate (a 60 wt% aqueous solution of LiBr) as a solvent.
33990049	6	6	theme	abundant	1271:1278	arg1	chitosan					1326:1333	chitosan	1326:1333	chitosan	1326:1333	This study successfully demonstrated an innovative method to fabricate biosorbents from abundant and renewable natural polymers (cellulose and chitosan) for removing metal ions from water.
33990049	6	6	theme	abundant	1271:1278	arg1	cellulose					1312:1320	cellulose	1312:1320	cellulose	1312:1320	This study successfully demonstrated an innovative method to fabricate biosorbents from abundant and renewable natural polymers (cellulose and chitosan) for removing metal ions from water.
33990049	6	6	theme	abundant	1271:1278	arg1	polymers					1302:1309	abundant and renewable natural polymers	1271:1309	abundant and renewable natural polymers (cellulose and chitosan)	1271:1334	This study successfully demonstrated an innovative method to fabricate biosorbents from abundant and renewable natural polymers (cellulose and chitosan) for removing metal ions from water.
33990049	0	7	theme	metals	127:132	arg1	biosorbent					107:116	biosorbent	107:116	biosorbent of heavy metals	107:132	Mesoporous cellulose-chitosan composite hydrogel fabricated via the co-dissolution-regeneration process as biosorbent of heavy metals.
33990049	4	8	theme	1500 mg/L	899:907	arg1	23 °C					851:855	23 °C	851:855	23 °C	851:855	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
33990049	4	8	theme	1500 mg/L	899:907	arg1	concentration					882:894	initial metal concentration	868:894	initial metal concentration	868:894	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
33990049	4	8	theme	1500 mg/L	899:907	arg1	pH					858:859	pH 5	858:861	pH 5	858:861	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
33990049	6	9	theme	renewable	1284:1292	arg1	chitosan					1326:1333	chitosan	1326:1333	chitosan	1326:1333	This study successfully demonstrated an innovative method to fabricate biosorbents from abundant and renewable natural polymers (cellulose and chitosan) for removing metal ions from water.
33990049	6	9	theme	renewable	1284:1292	arg1	cellulose					1312:1320	cellulose	1312:1320	cellulose	1312:1320	This study successfully demonstrated an innovative method to fabricate biosorbents from abundant and renewable natural polymers (cellulose and chitosan) for removing metal ions from water.
33990049	6	9	theme	renewable	1284:1292	arg1	polymers					1302:1309	abundant and renewable natural polymers	1271:1309	abundant and renewable natural polymers (cellulose and chitosan)	1271:1334	This study successfully demonstrated an innovative method to fabricate biosorbents from abundant and renewable natural polymers (cellulose and chitosan) for removing metal ions from water.
33990049	2	10	theme	regeneration	348:359	arg1	process					361:367	a co-dissolution and regeneration process	327:367	process	361:367	In this study, cellulose-chitosan composite hydrogels were fabricated via a co-dissolution and regeneration process using a molten salt hydrate (a 60 wt% aqueous solution of LiBr) as a solvent.
33990049	0	11	theme	heavy	121:125	arg1	metals					127:132	heavy metals	121:132	heavy metals	121:132	Mesoporous cellulose-chitosan composite hydrogel fabricated via the co-dissolution-regeneration process as biosorbent of heavy metals.
33990049	4	12	theme	%	747:747	arg1	cellulose					749:757	37% cellulose	745:757	37% cellulose	745:757	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
33990049	4	13	theme	63	763:764	arg1	%					765:765	%	765:765	%	765:765	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
33990049	4	14	theme	37	745:746	arg1	%					747:747	%	747:747	%	747:747	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
33990049	5	15	theme	metals	1140:1145	arg1	adsorption					1122:1131	selective adsorption	1112:1131	selective adsorption of the metals	1112:1145	Competitive adsorption from a mixed metals solution revealed that the cellulose-chitosan composite hydrogel exhibited selective adsorption of the metals in the order of Cu2+ > Zn2+ > Co2+.
33990049	2	16	theme	co-dissolution	329:342	arg1	process					361:367	a co-dissolution and regeneration process	327:367	process	361:367	In this study, cellulose-chitosan composite hydrogels were fabricated via a co-dissolution and regeneration process using a molten salt hydrate (a 60 wt% aqueous solution of LiBr) as a solvent.
33990049	4	17	theme	composite	721:729	arg1	hydrogel					731:738	the composite hydrogel	717:738	the composite hydrogel with 37% cellulose and 63% chitosan	717:774	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
33990049	3	18	theme	chitosan	463:470	arg1	addition					451:458	The addition	447:458	The addition of chitosan	447:470	The addition of chitosan not only introduced functionality for metal adsorption but also increased the specific surface area and improved the mechanical strength of the composite hydrogel, compared to pure cellulose hydrogel.
33990049	4	19	theme	94.3 mg/g	812:820	arg1	capacity					800:807	an adsorption capacity	786:807	an adsorption capacity of 94.3 mg/g (1.49 mmol/g)	786:834	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
33990049	1	20	theme	more	228:231	arg1	attention					242:250	more and more attention	228:250	more and more attention	228:250	Developing low-cost and high-performance biosorbent for water purification continues drawing more and more attention.
33990049	0	21	theme	cellulose-chitosan	11:28	arg1	hydrogel					40:47	Mesoporous cellulose-chitosan composite hydrogel	0:47	Mesoporous cellulose-chitosan composite hydrogel	0:47	Mesoporous cellulose-chitosan composite hydrogel fabricated via the co-dissolution-regeneration process as biosorbent of heavy metals.
33990049	3	22	theme	surface	559:565	arg1	area					567:570	the specific surface area	546:570	the specific surface area	546:570	The addition of chitosan not only introduced functionality for metal adsorption but also increased the specific surface area and improved the mechanical strength of the composite hydrogel, compared to pure cellulose hydrogel.
33990049	4	23	theme	initial	868:874	arg1	concentration					882:894	initial metal concentration	868:894	initial metal concentration	868:894	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
33990049	2	24	theme	molten	377:382	arg1	solvent					438:444	a solvent	436:444	a solvent	436:444	In this study, cellulose-chitosan composite hydrogels were fabricated via a co-dissolution and regeneration process using a molten salt hydrate (a 60 wt% aqueous solution of LiBr) as a solvent.
33990049	2	24	theme	molten	377:382	arg1	hydrate					389:395	a molten salt hydrate	375:395	a molten salt hydrate (a 60 wt% aqueous solution of LiBr)	375:431	In this study, cellulose-chitosan composite hydrogels were fabricated via a co-dissolution and regeneration process using a molten salt hydrate (a 60 wt% aqueous solution of LiBr) as a solvent.
33990049	2	25	dep	hydrate	389:395	arg1	solution					415:422	a 60 wt% aqueous solution	398:422	a 60 wt% aqueous solution of LiBr	398:430	In this study, cellulose-chitosan composite hydrogels were fabricated via a co-dissolution and regeneration process using a molten salt hydrate (a 60 wt% aqueous solution of LiBr) as a solvent.
33990049	2	26	theme	LiBr	427:430	arg1	solution					415:422	a 60 wt% aqueous solution	398:422	a 60 wt% aqueous solution of LiBr	398:430	In this study, cellulose-chitosan composite hydrogels were fabricated via a co-dissolution and regeneration process using a molten salt hydrate (a 60 wt% aqueous solution of LiBr) as a solvent.
33990049	0	27	theme	Mesoporous	0:9	arg1	hydrogel					40:47	Mesoporous cellulose-chitosan composite hydrogel	0:47	Mesoporous cellulose-chitosan composite hydrogel	0:47	Mesoporous cellulose-chitosan composite hydrogel fabricated via the co-dissolution-regeneration process as biosorbent of heavy metals.
33990049	1	28	theme	more	237:240	arg1	attention					242:250	more and more attention	228:250	more and more attention	228:250	Developing low-cost and high-performance biosorbent for water purification continues drawing more and more attention.
33990049	4	29	theme	greater	929:935	arg1	times					923:927	10 times	920:927	10 times greater than the adsorption capacity of pure cellulose hydrogel	920:991	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
33990049	3	30	theme	hydrogel	626:633	arg1	strength					600:607	the mechanical strength	585:607	the mechanical strength of the composite hydrogel	585:633	The addition of chitosan not only introduced functionality for metal adsorption but also increased the specific surface area and improved the mechanical strength of the composite hydrogel, compared to pure cellulose hydrogel.
33990049	3	31	theme	mechanical	589:598	arg1	strength					600:607	the mechanical strength	585:607	the mechanical strength of the composite hydrogel	585:633	The addition of chitosan not only introduced functionality for metal adsorption but also increased the specific surface area and improved the mechanical strength of the composite hydrogel, compared to pure cellulose hydrogel.
33990049	5	32	theme	metals	1030:1035	arg1	solution					1037:1044	a mixed metals solution	1022:1044	a mixed metals solution	1022:1044	Competitive adsorption from a mixed metals solution revealed that the cellulose-chitosan composite hydrogel exhibited selective adsorption of the metals in the order of Cu2+ > Zn2+ > Co2+.
33990049	4	33	theme	Batch	673:677	arg1	experiments					690:700	Batch adsorption experiments	673:700	Batch adsorption experiments	673:700	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
33990049	0	34	theme	composite	30:38	arg1	hydrogel					40:47	Mesoporous cellulose-chitosan composite hydrogel	0:47	Mesoporous cellulose-chitosan composite hydrogel	0:47	Mesoporous cellulose-chitosan composite hydrogel fabricated via the co-dissolution-regeneration process as biosorbent of heavy metals.
33990049	6	35	theme	innovative	1223:1232	arg1	method					1234:1239	an innovative method	1220:1239	an innovative method to fabricate biosorbents from abundant and renewable natural polymers (cellulose and chitosan) for removing metal ions from water	1220:1369	This study successfully demonstrated an innovative method to fabricate biosorbents from abundant and renewable natural polymers (cellulose and chitosan) for removing metal ions from water.
33990049	1	36	theme	Developing	135:144	arg1	biosorbent					176:185	Developing low-cost and high-performance biosorbent	135:185	Developing low-cost and high-performance biosorbent for water purification	135:208	Developing low-cost and high-performance biosorbent for water purification continues drawing more and more attention.
33990049	5	37	theme	selective	1112:1120	arg1	adsorption					1122:1131	selective adsorption	1112:1131	selective adsorption of the metals	1112:1145	Competitive adsorption from a mixed metals solution revealed that the cellulose-chitosan composite hydrogel exhibited selective adsorption of the metals in the order of Cu2+ > Zn2+ > Co2+.
33990049	2	38	theme	composite	287:295	arg1	hydrogels					297:305	cellulose-chitosan composite hydrogels	268:305	cellulose-chitosan composite hydrogels	268:305	In this study, cellulose-chitosan composite hydrogels were fabricated via a co-dissolution and regeneration process using a molten salt hydrate (a 60 wt% aqueous solution of LiBr) as a solvent.
33990049	5	39	from	solution	1037:1044	arg1	adsorption					1006:1015	Competitive adsorption	994:1015	Competitive adsorption from a mixed metals solution	994:1044	Competitive adsorption from a mixed metals solution revealed that the cellulose-chitosan composite hydrogel exhibited selective adsorption of the metals in the order of Cu2+ > Zn2+ > Co2+.
33990049	6	40	dep	polymers	1302:1309	arg1	chitosan					1326:1333	chitosan	1326:1333	chitosan	1326:1333	This study successfully demonstrated an innovative method to fabricate biosorbents from abundant and renewable natural polymers (cellulose and chitosan) for removing metal ions from water.
33990049	6	40	dep	polymers	1302:1309	arg1	cellulose					1312:1320	cellulose	1312:1320	cellulose	1312:1320	This study successfully demonstrated an innovative method to fabricate biosorbents from abundant and renewable natural polymers (cellulose and chitosan) for removing metal ions from water.
33990049	6	40	dep	polymers	1302:1309	arg1	polymers					1302:1309	abundant and renewable natural polymers	1271:1309	abundant and renewable natural polymers (cellulose and chitosan)	1271:1334	This study successfully demonstrated an innovative method to fabricate biosorbents from abundant and renewable natural polymers (cellulose and chitosan) for removing metal ions from water.
33990049	2	41	theme	cellulose-chitosan	268:285	arg1	hydrogels					297:305	cellulose-chitosan composite hydrogels	268:305	cellulose-chitosan composite hydrogels	268:305	In this study, cellulose-chitosan composite hydrogels were fabricated via a co-dissolution and regeneration process using a molten salt hydrate (a 60 wt% aqueous solution of LiBr) as a solvent.
33990049	4	42	theme	adsorption	679:688	arg1	experiments					690:700	Batch adsorption experiments	673:700	Batch adsorption experiments	673:700	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
33990049	0	43	theme	co-dissolution-regeneration	68:94	arg1	process					96:102	the co-dissolution-regeneration process	64:102	the co-dissolution-regeneration process as biosorbent of heavy metals	64:132	Mesoporous cellulose-chitosan composite hydrogel fabricated via the co-dissolution-regeneration process as biosorbent of heavy metals.
33990049	3	44	theme	composite	616:624	arg1	hydrogel					626:633	the composite hydrogel	612:633	the composite hydrogel	612:633	The addition of chitosan not only introduced functionality for metal adsorption but also increased the specific surface area and improved the mechanical strength of the composite hydrogel, compared to pure cellulose hydrogel.
33990049	1	45	theme	low-cost	146:153	arg1	biosorbent					176:185	Developing low-cost and high-performance biosorbent	135:185	Developing low-cost and high-performance biosorbent for water purification	135:208	Developing low-cost and high-performance biosorbent for water purification continues drawing more and more attention.
33990049	1	46	theme	water	191:195	arg1	purification					197:208	water purification	191:208	water purification	191:208	Developing low-cost and high-performance biosorbent for water purification continues drawing more and more attention.
33990049	4	47	theme	%	765:765	arg1	chitosan					767:774	63% chitosan	763:774	63% chitosan	763:774	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
33990049	4	48	theme	pure	969:972	arg1	hydrogel					984:991	pure cellulose hydrogel	969:991	pure cellulose hydrogel	969:991	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
33990049	5	49	theme	mixed	1024:1028	arg1	solution					1037:1044	a mixed metals solution	1022:1044	a mixed metals solution	1022:1044	Competitive adsorption from a mixed metals solution revealed that the cellulose-chitosan composite hydrogel exhibited selective adsorption of the metals in the order of Cu2+ > Zn2+ > Co2+.
33990049	5	50	theme	cellulose-chitosan	1064:1081	arg1	hydrogel					1093:1100	the cellulose-chitosan composite hydrogel	1060:1100	the cellulose-chitosan composite hydrogel	1060:1100	Competitive adsorption from a mixed metals solution revealed that the cellulose-chitosan composite hydrogel exhibited selective adsorption of the metals in the order of Cu2+ > Zn2+ > Co2+.
33990049	3	51	theme	cellulose	653:661	arg1	hydrogel					663:670	pure cellulose hydrogel	648:670	pure cellulose hydrogel	648:670	The addition of chitosan not only introduced functionality for metal adsorption but also increased the specific surface area and improved the mechanical strength of the composite hydrogel, compared to pure cellulose hydrogel.
33990049	6	52	theme	natural	1294:1300	arg1	chitosan					1326:1333	chitosan	1326:1333	chitosan	1326:1333	This study successfully demonstrated an innovative method to fabricate biosorbents from abundant and renewable natural polymers (cellulose and chitosan) for removing metal ions from water.
33990049	6	52	theme	natural	1294:1300	arg1	cellulose					1312:1320	cellulose	1312:1320	cellulose	1312:1320	This study successfully demonstrated an innovative method to fabricate biosorbents from abundant and renewable natural polymers (cellulose and chitosan) for removing metal ions from water.
33990049	6	52	theme	natural	1294:1300	arg1	polymers					1302:1309	abundant and renewable natural polymers	1271:1309	abundant and renewable natural polymers (cellulose and chitosan)	1271:1334	This study successfully demonstrated an innovative method to fabricate biosorbents from abundant and renewable natural polymers (cellulose and chitosan) for removing metal ions from water.
33990049	1	53	theme	high-performance	159:174	arg1	biosorbent					176:185	Developing low-cost and high-performance biosorbent	135:185	Developing low-cost and high-performance biosorbent for water purification	135:208	Developing low-cost and high-performance biosorbent for water purification continues drawing more and more attention.
33990049	4	54	theme	adsorption	789:798	arg1	capacity					800:807	an adsorption capacity	786:807	an adsorption capacity of 94.3 mg/g (1.49 mmol/g)	786:834	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
33990049	5	55	theme	Competitive	994:1004	arg1	adsorption					1006:1015	Competitive adsorption	994:1015	Competitive adsorption from a mixed metals solution	994:1044	Competitive adsorption from a mixed metals solution revealed that the cellulose-chitosan composite hydrogel exhibited selective adsorption of the metals in the order of Cu2+ > Zn2+ > Co2+.
33990049	5	56	theme	composite	1083:1091	arg1	hydrogel					1093:1100	the cellulose-chitosan composite hydrogel	1060:1100	the cellulose-chitosan composite hydrogel	1060:1100	Competitive adsorption from a mixed metals solution revealed that the cellulose-chitosan composite hydrogel exhibited selective adsorption of the metals in the order of Cu2+ > Zn2+ > Co2+.
33990049	2	57	theme	salt	384:387	arg1	solvent					438:444	a solvent	436:444	a solvent	436:444	In this study, cellulose-chitosan composite hydrogels were fabricated via a co-dissolution and regeneration process using a molten salt hydrate (a 60 wt% aqueous solution of LiBr) as a solvent.
33990049	2	57	theme	salt	384:387	arg1	hydrate					389:395	a molten salt hydrate	375:395	a molten salt hydrate (a 60 wt% aqueous solution of LiBr)	375:431	In this study, cellulose-chitosan composite hydrogels were fabricated via a co-dissolution and regeneration process using a molten salt hydrate (a 60 wt% aqueous solution of LiBr) as a solvent.
33990049	6	58	from	polymers	1302:1309	arg1	biosorbents					1254:1264	biosorbents	1254:1264	biosorbents from abundant and renewable natural polymers (cellulose and chitosan)	1254:1334	This study successfully demonstrated an innovative method to fabricate biosorbents from abundant and renewable natural polymers (cellulose and chitosan) for removing metal ions from water.
33990049	3	59	theme	metal	510:514	arg1	adsorption					516:525	metal adsorption	510:525	metal adsorption	510:525	The addition of chitosan not only introduced functionality for metal adsorption but also increased the specific surface area and improved the mechanical strength of the composite hydrogel, compared to pure cellulose hydrogel.
33990049	4	60	theme	hydrogel	984:991	arg1	capacity					957:964	the adsorption capacity	942:964	the adsorption capacity of pure cellulose hydrogel	942:991	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
33990049	3	61	theme	specific	550:557	arg1	area					567:570	the specific surface area	546:570	the specific surface area	546:570	The addition of chitosan not only introduced functionality for metal adsorption but also increased the specific surface area and improved the mechanical strength of the composite hydrogel, compared to pure cellulose hydrogel.
33990049	4	62	theme	cellulose	974:982	arg1	hydrogel					984:991	pure cellulose hydrogel	969:991	pure cellulose hydrogel	969:991	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
33990049	3	63	theme	pure	648:651	arg1	hydrogel					663:670	pure cellulose hydrogel	648:670	pure cellulose hydrogel	648:670	The addition of chitosan not only introduced functionality for metal adsorption but also increased the specific surface area and improved the mechanical strength of the composite hydrogel, compared to pure cellulose hydrogel.
33990049	2	64	theme	aqueous	407:413	arg1	solution					415:422	a 60 wt% aqueous solution	398:422	a 60 wt% aqueous solution of LiBr	398:430	In this study, cellulose-chitosan composite hydrogels were fabricated via a co-dissolution and regeneration process using a molten salt hydrate (a 60 wt% aqueous solution of LiBr) as a solvent.
33990049	4	65	theme	adsorption	946:955	arg1	capacity					957:964	the adsorption capacity	942:964	the adsorption capacity of pure cellulose hydrogel	942:991	Batch adsorption experiments indicated that the composite hydrogel with 37% cellulose and 63% chitosan exhibited an adsorption capacity of 94.3 mg/g (1.49 mmol/g) toward Cu2+ at 23 °C, pH 5, and initial metal concentration of 1500 mg/L, which was 10 times greater than the adsorption capacity of pure cellulose hydrogel.
32919637	4	0	theme	excellent	846:854	arg1	compatibility					861:873	the excellent cell compatibility	842:873	the excellent cell compatibility	842:873	The as-prepared composite hydrogels show the excellent cell compatibility and low toxicity.
32919637	2	1	theme	Insulin-loaded	356:369	arg1	ILM					381:383	ILM	381:383	ILM	381:383	Insulin-loaded micelles (ILM) and epidermal growth factor (EGF) are further embedded into the composite hydrogels that can be rapidly gelled by mixing of oxidized hyaluronic acid (OHA) and succinyl chitosan (SCS).
32919637	2	1	theme	Insulin-loaded	356:369	arg1	micelles					371:378	Insulin-loaded micelles	356:378	Insulin-loaded micelles (ILM)	356:384	Insulin-loaded micelles (ILM) and epidermal growth factor (EGF) are further embedded into the composite hydrogels that can be rapidly gelled by mixing of oxidized hyaluronic acid (OHA) and succinyl chitosan (SCS).
32919637	3	2	theme	hydrogels	681:689	arg1	properties					641:650	the morphology, rheology, degradation, swelling and cytotoxicity properties	576:650	the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels	576:689	Then, the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels are further investigated to evaluate their physical properties and biocompatibility of as the wound dressing.
32919637	2	3	theme	acid	530:533	arg1	mixing					500:505	mixing	500:505	mixing of oxidized hyaluronic acid (OHA) and succinyl chitosan (SCS)	500:567	Insulin-loaded micelles (ILM) and epidermal growth factor (EGF) are further embedded into the composite hydrogels that can be rapidly gelled by mixing of oxidized hyaluronic acid (OHA) and succinyl chitosan (SCS).
32919637	1	4	theme	insulin-loaded	331:344	arg1	micelles					346:353	insulin-loaded micelles	331:353	insulin-loaded micelles	331:353	In this work, poly(ethylene glycol)-b-poly[3-acrylamidophenylboronic acid-co-styrene] (PEG-b-P(PBA-co-St) has been firstly synthesized for loading of insulin to form insulin-loaded micelles.
32919637	5	5	theme	rat	1042:1044	arg1	models					1046:1051	streptozotocin-induced rat models	1019:1051	streptozotocin-induced rat models	1019:1051	To evaluate the wound healing ability of as-prepared composite hydrogels, the tests of wound healing in vivo are conducted on streptozotocin-induced rat models.
32919637	4	6	theme	cell	856:859	arg1	compatibility					861:873	the excellent cell compatibility	842:873	the excellent cell compatibility	842:873	The as-prepared composite hydrogels show the excellent cell compatibility and low toxicity.
32919637	7	7	theme	as-prepared	1323:1333	arg1	hydrogels					1345:1353	the as-prepared composite hydrogels	1319:1353	the as-prepared composite hydrogels with loading of ILM and EGF	1319:1381	These results suggest that the as-prepared composite hydrogels with loading of ILM and EGF could be a promising candidate for wound healing applications.
32919637	7	7	theme	as-prepared	1323:1333	arg1	candidate					1404:1412	a promising candidate	1392:1412	a promising candidate for wound healing applications	1392:1443	These results suggest that the as-prepared composite hydrogels with loading of ILM and EGF could be a promising candidate for wound healing applications.
32919637	6	8	theme	myofibrils	1280:1289	arg1	integrity					1227:1235	tissue internal structure integrity	1201:1235	tissue internal structure integrity	1201:1235	And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils.
32919637	6	8	theme	myofibrils	1280:1289	arg1	proliferation					1183:1195	fibroblast proliferation	1172:1195	fibroblast proliferation	1172:1195	And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils.
32919637	6	8	theme	myofibrils	1280:1289	arg1	deposition					1253:1262	the deposition	1249:1262	the deposition of collagen and myofibrils	1249:1289	And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils.
32919637	6	9	theme	tissue	1201:1206	arg1	integrity					1227:1235	tissue internal structure integrity	1201:1235	tissue internal structure integrity	1201:1235	And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils.
32919637	6	10	theme	collagen	1267:1274	arg1	integrity					1227:1235	tissue internal structure integrity	1201:1235	tissue internal structure integrity	1201:1235	And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils.
32919637	6	10	theme	collagen	1267:1274	arg1	proliferation					1183:1195	fibroblast proliferation	1172:1195	fibroblast proliferation	1172:1195	And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils.
32919637	6	10	theme	collagen	1267:1274	arg1	deposition					1253:1262	the deposition	1249:1262	the deposition of collagen and myofibrils	1249:1289	And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils.
32919637	0	11	with	integration	69:79	arg1	micelles					101:108	insulin-loaded micelles	86:108	insulin-loaded micelles	86:108	Rapid gelation of oxidized hyaluronic acid and succinyl chitosan for integration with insulin-loaded micelles and epidermal growth factor on diabetic wound healing.
32919637	0	11	with	integration	69:79	arg1	factor					131:136	epidermal growth factor	114:136	epidermal growth factor	114:136	Rapid gelation of oxidized hyaluronic acid and succinyl chitosan for integration with insulin-loaded micelles and epidermal growth factor on diabetic wound healing.
32919637	5	12	theme	composite	946:954	arg1	hydrogels					956:964	as-prepared composite hydrogels	934:964	as-prepared composite hydrogels	934:964	To evaluate the wound healing ability of as-prepared composite hydrogels, the tests of wound healing in vivo are conducted on streptozotocin-induced rat models.
32919637	6	13	theme	excellent	1119:1127	arg1	healing					1135:1141	an excellent wound healing	1116:1141	an excellent wound healing performance	1116:1153	And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils.
32919637	3	14	theme	wound	785:789	arg1	dressing					791:798	the wound dressing	781:798	the wound dressing	781:798	Then, the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels are further investigated to evaluate their physical properties and biocompatibility of as the wound dressing.
32919637	3	14	theme	wound	785:789	arg1	biocompatibility					758:773	biocompatibility	758:773	biocompatibility of	758:776	Then, the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels are further investigated to evaluate their physical properties and biocompatibility of as the wound dressing.
32919637	3	14	theme	wound	785:789	arg1	properties					743:752	their physical properties	728:752	their physical properties	728:752	Then, the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels are further investigated to evaluate their physical properties and biocompatibility of as the wound dressing.
32919637	0	15	theme	insulin-loaded	86:99	arg1	micelles					101:108	insulin-loaded micelles	86:108	insulin-loaded micelles	86:108	Rapid gelation of oxidized hyaluronic acid and succinyl chitosan for integration with insulin-loaded micelles and epidermal growth factor on diabetic wound healing.
32919637	3	16	theme	cytotoxicity	628:639	arg1	properties					641:650	the morphology, rheology, degradation, swelling and cytotoxicity properties	576:650	the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels	576:689	Then, the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels are further investigated to evaluate their physical properties and biocompatibility of as the wound dressing.
32919637	2	17	theme	chitosan	554:561	arg1	mixing					500:505	mixing	500:505	mixing of oxidized hyaluronic acid (OHA) and succinyl chitosan (SCS)	500:567	Insulin-loaded micelles (ILM) and epidermal growth factor (EGF) are further embedded into the composite hydrogels that can be rapidly gelled by mixing of oxidized hyaluronic acid (OHA) and succinyl chitosan (SCS).
32919637	5	18	theme	hydrogels	956:964	arg1	ability					923:929	the wound healing ability	905:929	the wound healing ability of as-prepared composite hydrogels	905:964	To evaluate the wound healing ability of as-prepared composite hydrogels, the tests of wound healing in vivo are conducted on streptozotocin-induced rat models.
32919637	7	19	theme	promising	1394:1402	arg1	hydrogels					1345:1353	the as-prepared composite hydrogels	1319:1353	the as-prepared composite hydrogels with loading of ILM and EGF	1319:1381	These results suggest that the as-prepared composite hydrogels with loading of ILM and EGF could be a promising candidate for wound healing applications.
32919637	7	19	theme	promising	1394:1402	arg1	candidate					1404:1412	a promising candidate	1392:1412	a promising candidate for wound healing applications	1392:1443	These results suggest that the as-prepared composite hydrogels with loading of ILM and EGF could be a promising candidate for wound healing applications.
32919637	0	20	theme	epidermal	114:122	arg1	factor					131:136	epidermal growth factor	114:136	epidermal growth factor	114:136	Rapid gelation of oxidized hyaluronic acid and succinyl chitosan for integration with insulin-loaded micelles and epidermal growth factor on diabetic wound healing.
32919637	4	21	theme	composite	817:825	arg1	hydrogels					827:835	The as-prepared composite hydrogels	801:835	The as-prepared composite hydrogels	801:835	The as-prepared composite hydrogels show the excellent cell compatibility and low toxicity.
32919637	6	22	theme	as-prepared	1062:1072	arg1	hydrogels					1084:1092	the as-prepared composite hydrogels	1058:1092	the as-prepared composite hydrogels with ILM	1058:1101	And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils.
32919637	2	23	theme	epidermal	390:398	arg1	factor					407:412	epidermal growth factor	390:412	epidermal growth factor (EGF)	390:418	Insulin-loaded micelles (ILM) and epidermal growth factor (EGF) are further embedded into the composite hydrogels that can be rapidly gelled by mixing of oxidized hyaluronic acid (OHA) and succinyl chitosan (SCS).
32919637	2	23	theme	epidermal	390:398	arg1	EGF					415:417	EGF	415:417	EGF	415:417	Insulin-loaded micelles (ILM) and epidermal growth factor (EGF) are further embedded into the composite hydrogels that can be rapidly gelled by mixing of oxidized hyaluronic acid (OHA) and succinyl chitosan (SCS).
32919637	3	24	theme	as-prepared	659:669	arg1	hydrogels					681:689	the as-prepared composite hydrogels	655:689	the as-prepared composite hydrogels	655:689	Then, the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels are further investigated to evaluate their physical properties and biocompatibility of as the wound dressing.
32919637	4	25	theme	as-prepared	805:815	arg1	hydrogels					827:835	The as-prepared composite hydrogels	801:835	The as-prepared composite hydrogels	801:835	The as-prepared composite hydrogels show the excellent cell compatibility and low toxicity.
32919637	7	26	theme	wound	1418:1422	arg1	healing					1424:1430	wound healing	1418:1430	wound healing applications	1418:1443	These results suggest that the as-prepared composite hydrogels with loading of ILM and EGF could be a promising candidate for wound healing applications.
32919637	6	27	theme	composite	1074:1082	arg1	hydrogels					1084:1092	the as-prepared composite hydrogels	1058:1092	the as-prepared composite hydrogels with ILM	1058:1101	And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils.
32919637	7	28	theme	composite	1335:1343	arg1	hydrogels					1345:1353	the as-prepared composite hydrogels	1319:1353	the as-prepared composite hydrogels with loading of ILM and EGF	1319:1381	These results suggest that the as-prepared composite hydrogels with loading of ILM and EGF could be a promising candidate for wound healing applications.
32919637	7	28	theme	composite	1335:1343	arg1	candidate					1404:1412	a promising candidate	1392:1412	a promising candidate for wound healing applications	1392:1443	These results suggest that the as-prepared composite hydrogels with loading of ILM and EGF could be a promising candidate for wound healing applications.
32919637	3	29	theme	composite	671:679	arg1	hydrogels					681:689	the as-prepared composite hydrogels	655:689	the as-prepared composite hydrogels	655:689	Then, the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels are further investigated to evaluate their physical properties and biocompatibility of as the wound dressing.
32919637	7	30	with	hydrogels	1345:1353	arg1	loading					1360:1366	loading	1360:1366	loading of ILM and EGF	1360:1381	These results suggest that the as-prepared composite hydrogels with loading of ILM and EGF could be a promising candidate for wound healing applications.
32919637	5	31	theme	healing	915:921	arg1	ability					923:929	the wound healing ability	905:929	the wound healing ability of as-prepared composite hydrogels	905:964	To evaluate the wound healing ability of as-prepared composite hydrogels, the tests of wound healing in vivo are conducted on streptozotocin-induced rat models.
32919637	0	32	theme	Rapid	0:4	arg1	gelation					6:13	Rapid gelation	0:13	Rapid gelation of oxidized hyaluronic acid and succinyl chitosan for integration with insulin-loaded micelles and epidermal growth factor on diabetic wound healing.	0:163	Rapid gelation of oxidized hyaluronic acid and succinyl chitosan for integration with insulin-loaded micelles and epidermal growth factor on diabetic wound healing.
32919637	6	33	theme	deposition	1253:1262	arg1	promotion					1159:1167	promotion	1159:1167	promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils	1159:1289	And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils.
32919637	6	34	theme	healing	1135:1141	arg1	performance					1143:1153	an excellent wound healing performance	1116:1153	an excellent wound healing performance	1116:1153	And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils.
32919637	0	35	theme	oxidized	18:25	arg1	acid					38:41	oxidized hyaluronic acid	18:41	oxidized hyaluronic acid	18:41	Rapid gelation of oxidized hyaluronic acid and succinyl chitosan for integration with insulin-loaded micelles and epidermal growth factor on diabetic wound healing.
32919637	6	36	theme	wound	1129:1133	arg1	healing					1135:1141	an excellent wound healing	1116:1141	an excellent wound healing performance	1116:1153	And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils.
32919637	0	37	theme	growth	124:129	arg1	factor					131:136	epidermal growth factor	114:136	epidermal growth factor	114:136	Rapid gelation of oxidized hyaluronic acid and succinyl chitosan for integration with insulin-loaded micelles and epidermal growth factor on diabetic wound healing.
32919637	3	38	theme	rheology	592:599	arg1	properties					641:650	the morphology, rheology, degradation, swelling and cytotoxicity properties	576:650	the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels	576:689	Then, the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels are further investigated to evaluate their physical properties and biocompatibility of as the wound dressing.
32919637	5	39	theme	as-prepared	934:944	arg1	hydrogels					956:964	as-prepared composite hydrogels	934:964	as-prepared composite hydrogels	934:964	To evaluate the wound healing ability of as-prepared composite hydrogels, the tests of wound healing in vivo are conducted on streptozotocin-induced rat models.
32919637	1	40	theme	ethylene	184:191	arg1	glycol					193:198	ethylene glycol	184:198	ethylene glycol	184:198	In this work, poly(ethylene glycol)-b-poly[3-acrylamidophenylboronic acid-co-styrene] (PEG-b-P(PBA-co-St) has been firstly synthesized for loading of insulin to form insulin-loaded micelles.
32919637	0	41	theme	acid	38:41	arg1	gelation					6:13	Rapid gelation	0:13	Rapid gelation of oxidized hyaluronic acid and succinyl chitosan for integration with insulin-loaded micelles and epidermal growth factor on diabetic wound healing.	0:163	Rapid gelation of oxidized hyaluronic acid and succinyl chitosan for integration with insulin-loaded micelles and epidermal growth factor on diabetic wound healing.
32919637	0	42	theme	diabetic	141:148	arg1	healing					156:162	diabetic wound healing	141:162	diabetic wound healing	141:162	Rapid gelation of oxidized hyaluronic acid and succinyl chitosan for integration with insulin-loaded micelles and epidermal growth factor on diabetic wound healing.
32919637	0	43	from	integration	69:79	arg1	healing					156:162	diabetic wound healing	141:162	diabetic wound healing	141:162	Rapid gelation of oxidized hyaluronic acid and succinyl chitosan for integration with insulin-loaded micelles and epidermal growth factor on diabetic wound healing.
32919637	7	44	theme	EGF	1379:1381	arg1	loading					1360:1366	loading	1360:1366	loading of ILM and EGF	1360:1381	These results suggest that the as-prepared composite hydrogels with loading of ILM and EGF could be a promising candidate for wound healing applications.
32919637	6	45	theme	proliferation	1183:1195	arg1	promotion					1159:1167	promotion	1159:1167	promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils	1159:1289	And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils.
32919637	0	46	theme	hyaluronic	27:36	arg1	acid					38:41	oxidized hyaluronic acid	18:41	oxidized hyaluronic acid	18:41	Rapid gelation of oxidized hyaluronic acid and succinyl chitosan for integration with insulin-loaded micelles and epidermal growth factor on diabetic wound healing.
32919637	2	47	theme	composite	450:458	arg1	hydrogels					460:468	the composite hydrogels	446:468	the composite hydrogels that can be rapidly gelled by mixing of oxidized hyaluronic acid (OHA) and succinyl chitosan (SCS)	446:567	Insulin-loaded micelles (ILM) and epidermal growth factor (EGF) are further embedded into the composite hydrogels that can be rapidly gelled by mixing of oxidized hyaluronic acid (OHA) and succinyl chitosan (SCS).
32919637	1	48	dep	-b-poly[3-acrylamidophenylboronic	200:232	arg1	glycol					193:198	ethylene glycol	184:198	ethylene glycol	184:198	In this work, poly(ethylene glycol)-b-poly[3-acrylamidophenylboronic acid-co-styrene] (PEG-b-P(PBA-co-St) has been firstly synthesized for loading of insulin to form insulin-loaded micelles.
32919637	7	49	theme	ILM	1371:1373	arg1	loading					1360:1366	loading	1360:1366	loading of ILM and EGF	1360:1381	These results suggest that the as-prepared composite hydrogels with loading of ILM and EGF could be a promising candidate for wound healing applications.
32919637	2	50	theme	growth	400:405	arg1	factor					407:412	epidermal growth factor	390:412	epidermal growth factor (EGF)	390:418	Insulin-loaded micelles (ILM) and epidermal growth factor (EGF) are further embedded into the composite hydrogels that can be rapidly gelled by mixing of oxidized hyaluronic acid (OHA) and succinyl chitosan (SCS).
32919637	2	50	theme	growth	400:405	arg1	EGF					415:417	EGF	415:417	EGF	415:417	Insulin-loaded micelles (ILM) and epidermal growth factor (EGF) are further embedded into the composite hydrogels that can be rapidly gelled by mixing of oxidized hyaluronic acid (OHA) and succinyl chitosan (SCS).
32919637	0	51	theme	succinyl	47:54	arg1	chitosan					56:63	succinyl chitosan	47:63	succinyl chitosan	47:63	Rapid gelation of oxidized hyaluronic acid and succinyl chitosan for integration with insulin-loaded micelles and epidermal growth factor on diabetic wound healing.
32919637	2	52	theme	succinyl	545:552	arg1	SCS					564:566	SCS	564:566	SCS	564:566	Insulin-loaded micelles (ILM) and epidermal growth factor (EGF) are further embedded into the composite hydrogels that can be rapidly gelled by mixing of oxidized hyaluronic acid (OHA) and succinyl chitosan (SCS).
32919637	2	52	theme	succinyl	545:552	arg1	chitosan					554:561	succinyl chitosan	545:561	succinyl chitosan (SCS)	545:567	Insulin-loaded micelles (ILM) and epidermal growth factor (EGF) are further embedded into the composite hydrogels that can be rapidly gelled by mixing of oxidized hyaluronic acid (OHA) and succinyl chitosan (SCS).
32919637	6	53	theme	integrity	1227:1235	arg1	promotion					1159:1167	promotion	1159:1167	promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils	1159:1289	And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils.
32919637	2	54	theme	oxidized	510:517	arg1	acid					530:533	oxidized hyaluronic acid	510:533	oxidized hyaluronic acid (OHA)	510:539	Insulin-loaded micelles (ILM) and epidermal growth factor (EGF) are further embedded into the composite hydrogels that can be rapidly gelled by mixing of oxidized hyaluronic acid (OHA) and succinyl chitosan (SCS).
32919637	2	54	theme	oxidized	510:517	arg1	OHA					536:538	OHA	536:538	OHA	536:538	Insulin-loaded micelles (ILM) and epidermal growth factor (EGF) are further embedded into the composite hydrogels that can be rapidly gelled by mixing of oxidized hyaluronic acid (OHA) and succinyl chitosan (SCS).
32919637	4	55	theme	low	879:881	arg1	toxicity					883:890	low toxicity	879:890	low toxicity	879:890	The as-prepared composite hydrogels show the excellent cell compatibility and low toxicity.
32919637	0	56	theme	wound	150:154	arg1	healing					156:162	diabetic wound healing	141:162	diabetic wound healing	141:162	Rapid gelation of oxidized hyaluronic acid and succinyl chitosan for integration with insulin-loaded micelles and epidermal growth factor on diabetic wound healing.
32919637	3	57	theme	swelling	615:622	arg1	properties					641:650	the morphology, rheology, degradation, swelling and cytotoxicity properties	576:650	the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels	576:689	Then, the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels are further investigated to evaluate their physical properties and biocompatibility of as the wound dressing.
32919637	6	58	theme	structure	1217:1225	arg1	integrity					1227:1235	tissue internal structure integrity	1201:1235	tissue internal structure integrity	1201:1235	And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils.
32919637	7	59	theme	healing	1424:1430	arg1	applications					1432:1443	wound healing applications	1418:1443	wound healing applications	1418:1443	These results suggest that the as-prepared composite hydrogels with loading of ILM and EGF could be a promising candidate for wound healing applications.
32919637	3	60	theme	morphology	580:589	arg1	properties					641:650	the morphology, rheology, degradation, swelling and cytotoxicity properties	576:650	the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels	576:689	Then, the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels are further investigated to evaluate their physical properties and biocompatibility of as the wound dressing.
32919637	1	61	theme	insulin	315:321	arg1	loading					304:310	loading	304:310	loading of insulin to form insulin-loaded micelles	304:353	In this work, poly(ethylene glycol)-b-poly[3-acrylamidophenylboronic acid-co-styrene] (PEG-b-P(PBA-co-St) has been firstly synthesized for loading of insulin to form insulin-loaded micelles.
32919637	5	62	theme	streptozotocin-induced	1019:1040	arg1	models					1046:1051	streptozotocin-induced rat models	1019:1051	streptozotocin-induced rat models	1019:1051	To evaluate the wound healing ability of as-prepared composite hydrogels, the tests of wound healing in vivo are conducted on streptozotocin-induced rat models.
32919637	6	63	theme	internal	1208:1215	arg1	integrity					1227:1235	tissue internal structure integrity	1201:1235	tissue internal structure integrity	1201:1235	And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils.
32919637	1	64	theme	-b-poly[3-acrylamidophenylboronic	200:232	arg1	PBA-co-St					260:268	PBA-co-St	260:268	PBA-co-St	260:268	In this work, poly(ethylene glycol)-b-poly[3-acrylamidophenylboronic acid-co-styrene] (PEG-b-P(PBA-co-St) has been firstly synthesized for loading of insulin to form insulin-loaded micelles.
32919637	1	64	theme	-b-poly[3-acrylamidophenylboronic	200:232	arg1	PEG-b-P					252:258	poly(ethylene glycol)-b-poly[3-acrylamidophenylboronic acid-co-styrene] (PEG-b-P	179:258	poly(ethylene glycol)-b-poly[3-acrylamidophenylboronic acid-co-styrene] (PEG-b-P(PBA-co-St)	179:269	In this work, poly(ethylene glycol)-b-poly[3-acrylamidophenylboronic acid-co-styrene] (PEG-b-P(PBA-co-St) has been firstly synthesized for loading of insulin to form insulin-loaded micelles.
32919637	3	65	theme	physical	734:741	arg1	dressing					791:798	the wound dressing	781:798	the wound dressing	781:798	Then, the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels are further investigated to evaluate their physical properties and biocompatibility of as the wound dressing.
32919637	3	65	theme	physical	734:741	arg1	biocompatibility					758:773	biocompatibility	758:773	biocompatibility of	758:776	Then, the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels are further investigated to evaluate their physical properties and biocompatibility of as the wound dressing.
32919637	3	65	theme	physical	734:741	arg1	properties					743:752	their physical properties	728:752	their physical properties	728:752	Then, the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels are further investigated to evaluate their physical properties and biocompatibility of as the wound dressing.
32919637	3	66	theme	degradation	602:612	arg1	properties					641:650	the morphology, rheology, degradation, swelling and cytotoxicity properties	576:650	the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels	576:689	Then, the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels are further investigated to evaluate their physical properties and biocompatibility of as the wound dressing.
32919637	6	67	theme	fibroblast	1172:1181	arg1	proliferation					1183:1195	fibroblast proliferation	1172:1195	fibroblast proliferation	1172:1195	And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils.
32919637	5	68	theme	wound	909:913	arg1	healing					915:921	the wound healing	905:921	the wound healing ability of as-prepared composite hydrogels	905:964	To evaluate the wound healing ability of as-prepared composite hydrogels, the tests of wound healing in vivo are conducted on streptozotocin-induced rat models.
32919637	1	69	theme	acid-co-styrene	234:248	arg1	PBA-co-St					260:268	PBA-co-St	260:268	PBA-co-St	260:268	In this work, poly(ethylene glycol)-b-poly[3-acrylamidophenylboronic acid-co-styrene] (PEG-b-P(PBA-co-St) has been firstly synthesized for loading of insulin to form insulin-loaded micelles.
32919637	1	69	theme	acid-co-styrene	234:248	arg1	PEG-b-P					252:258	poly(ethylene glycol)-b-poly[3-acrylamidophenylboronic acid-co-styrene] (PEG-b-P	179:258	poly(ethylene glycol)-b-poly[3-acrylamidophenylboronic acid-co-styrene] (PEG-b-P(PBA-co-St)	179:269	In this work, poly(ethylene glycol)-b-poly[3-acrylamidophenylboronic acid-co-styrene] (PEG-b-P(PBA-co-St) has been firstly synthesized for loading of insulin to form insulin-loaded micelles.
32919637	6	70	with	hydrogels	1084:1092	arg1	ILM					1099:1101	ILM	1099:1101	ILM	1099:1101	And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils.
32919637	5	71	theme	wound	980:984	arg1	healing					986:992	wound healing	980:992	wound healing	980:992	To evaluate the wound healing ability of as-prepared composite hydrogels, the tests of wound healing in vivo are conducted on streptozotocin-induced rat models.
32919637	0	72	theme	chitosan	56:63	arg1	gelation					6:13	Rapid gelation	0:13	Rapid gelation of oxidized hyaluronic acid and succinyl chitosan for integration with insulin-loaded micelles and epidermal growth factor on diabetic wound healing.	0:163	Rapid gelation of oxidized hyaluronic acid and succinyl chitosan for integration with insulin-loaded micelles and epidermal growth factor on diabetic wound healing.
32919637	6	73	with	EGF	1107:1109	arg1	ILM					1099:1101	ILM	1099:1101	ILM	1099:1101	And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils.
32919637	5	74	theme	healing	986:992	arg1	tests					971:975	the tests	967:975	the tests of wound healing	967:992	To evaluate the wound healing ability of as-prepared composite hydrogels, the tests of wound healing in vivo are conducted on streptozotocin-induced rat models.
32919637	2	75	theme	hyaluronic	519:528	arg1	acid					530:533	oxidized hyaluronic acid	510:533	oxidized hyaluronic acid (OHA)	510:539	Insulin-loaded micelles (ILM) and epidermal growth factor (EGF) are further embedded into the composite hydrogels that can be rapidly gelled by mixing of oxidized hyaluronic acid (OHA) and succinyl chitosan (SCS).
32919637	2	75	theme	hyaluronic	519:528	arg1	OHA					536:538	OHA	536:538	OHA	536:538	Insulin-loaded micelles (ILM) and epidermal growth factor (EGF) are further embedded into the composite hydrogels that can be rapidly gelled by mixing of oxidized hyaluronic acid (OHA) and succinyl chitosan (SCS).
31905423	7	0	theme	milk	1151:1154	arg1	proteins					1156:1163	milk proteins	1151:1163	milk proteins	1151:1163	PRACTICAL APPLICATIONS: PHM, NabM, and their complexes with milk proteins were proved to improve liver function, enhancing most of its measurable parameters and also diminishing the risk of cardiovascular disease.
31905423	2	1	theme	ispaghula	377:385	arg1	PHM					398:400	PHM	398:400	PHM	398:400	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	2	1	theme	ispaghula	377:385	arg1	mucilage					388:395	Psyllium husk (ispaghula) mucilage	362:395	Psyllium husk (ispaghula) mucilage (PHM)	362:401	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	9	2	theme	liver	1684:1688	arg1	function					1690:1697	the whole liver function	1674:1697	the whole liver function	1674:1697	Knowing how much these diseases proliferate in the western world and its correlation with high-fat consumption and modern lifestyle, its conjugation with PHM/NabM-MP complexes may reduce the negative impact of unhealthy food intake and, on some parameters, even improving the whole liver function.
31905423	8	3	with	Mice	1305:1308	arg1	HFD					1315:1317	HFD	1315:1317	HFD	1315:1317	Mice with HFD achieved better health circumstances by combining these ingredients in their diet.
31905423	11	4	theme	many	1895:1898	arg1	interactions					1900:1911	many interactions	1895:1911	many interactions concerning milk proteins	1895:1936	Although many interactions concerning milk proteins have already been analyzed, our study also proposes the interaction with bioactive polysaccharides as useful, opening a field of research aimed at the better application of milk proteins.
31905423	2	5	theme	protein	298:304	arg1	WPH					319:321	WPH	319:321	WPH	319:321	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	2	5	theme	protein	298:304	arg1	hydrolysate					306:316	whey protein hydrolysate	293:316	whey protein hydrolysate (WPH)	293:322	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	11	6	dep	proposes	1981:1988	arg1	opening					2048:2054	opening	2048:2054	opening	2048:2054	Although many interactions concerning milk proteins have already been analyzed, our study also proposes the interaction with bioactive polysaccharides as useful, opening a field of research aimed at the better application of milk proteins.
31905423	2	7	theme	noncovalent	332:342	arg1	interactions					344:355	noncovalent interactions	332:355	noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM)	332:426	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	5	8	theme	histopathological	891:907	arg1	examination					909:919	the liver histopathological examination	881:919	the liver histopathological examination	881:919	The results showed that the complexes exerted significant effect on normalizing tested parameters; WPH-NabM had the most significantly decreased level of malondialdehyde content and the liver histopathological examination proved an improvement in all groups fed with these complexes.
31905423	11	9	theme	better	2089:2094	arg1	application					2096:2106	the better application	2085:2106	the better application of milk proteins	2085:2123	Although many interactions concerning milk proteins have already been analyzed, our study also proposes the interaction with bioactive polysaccharides as useful, opening a field of research aimed at the better application of milk proteins.
31905423	9	10	theme	unhealthy	1612:1620	arg1	intake					1627:1632	unhealthy food intake	1612:1632	unhealthy food intake	1612:1632	Knowing how much these diseases proliferate in the western world and its correlation with high-fat consumption and modern lifestyle, its conjugation with PHM/NabM-MP complexes may reduce the negative impact of unhealthy food intake and, on some parameters, even improving the whole liver function.
31905423	3	11	theme	complexes	521:529	arg1	composition					438:448	Chemical composition	429:448	Chemical composition	429:448	Chemical composition, phenolic content, and antioxidant activities of milk protein-mucilage complexes were explored.
31905423	3	11	theme	complexes	521:529	arg1	content					460:466	phenolic content	451:466	phenolic content	451:466	Chemical composition, phenolic content, and antioxidant activities of milk protein-mucilage complexes were explored.
31905423	3	11	theme	complexes	521:529	arg1	activities					485:494	antioxidant activities	473:494	antioxidant activities	473:494	Chemical composition, phenolic content, and antioxidant activities of milk protein-mucilage complexes were explored.
31905423	2	12	theme	buffalo	261:267	arg1	proteins					280:287	buffalo total milk proteins	261:287	buffalo total milk proteins	261:287	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	9	13	theme	modern	1517:1522	arg1	lifestyle					1524:1532	modern lifestyle	1517:1532	modern lifestyle	1517:1532	Knowing how much these diseases proliferate in the western world and its correlation with high-fat consumption and modern lifestyle, its conjugation with PHM/NabM-MP complexes may reduce the negative impact of unhealthy food intake and, on some parameters, even improving the whole liver function.
31905423	11	14	theme	proteins	2116:2123	arg1	application					2096:2106	the better application	2085:2106	the better application of milk proteins	2085:2123	Although many interactions concerning milk proteins have already been analyzed, our study also proposes the interaction with bioactive polysaccharides as useful, opening a field of research aimed at the better application of milk proteins.
31905423	8	15	theme	better	1328:1333	arg1	circumstances					1342:1354	better health circumstances	1328:1354	better health circumstances	1328:1354	Mice with HFD achieved better health circumstances by combining these ingredients in their diet.
31905423	10	16	theme	supplements	1851:1861	arg1	consumption					1785:1795	consumption	1785:1795	consumption	1785:1795	For that reason, the present study supports and pushes forward the dissemination and consumption of ispaghula or Nabq (the polysaccharides sources) or supplements originating from them.
31905423	10	16	theme	supplements	1851:1861	arg1	dissemination					1767:1779	dissemination	1767:1779	dissemination	1767:1779	For that reason, the present study supports and pushes forward the dissemination and consumption of ispaghula or Nabq (the polysaccharides sources) or supplements originating from them.
31905423	9	17	dep	reduce	1582:1587	arg1	improving					1664:1672	improving	1664:1672	even improving the whole liver function	1659:1697	Knowing how much these diseases proliferate in the western world and its correlation with high-fat consumption and modern lifestyle, its conjugation with PHM/NabM-MP complexes may reduce the negative impact of unhealthy food intake and, on some parameters, even improving the whole liver function.
31905423	10	18	dep	dissemination	1767:1779	arg1	the					1763:1765	the	1763:1765	the	1763:1765	For that reason, the present study supports and pushes forward the dissemination and consumption of ispaghula or Nabq (the polysaccharides sources) or supplements originating from them.
31905423	5	19	theme	decreased	834:842	arg1	level					844:848	the most significantly decreased level	811:848	the most significantly decreased level of malondialdehyde content	811:875	The results showed that the complexes exerted significant effect on normalizing tested parameters; WPH-NabM had the most significantly decreased level of malondialdehyde content and the liver histopathological examination proved an improvement in all groups fed with these complexes.
31905423	9	20	theme	PHM/NabM-MP	1556:1566	arg1	complexes					1568:1576	PHM/NabM-MP complexes	1556:1576	PHM/NabM-MP complexes	1556:1576	Knowing how much these diseases proliferate in the western world and its correlation with high-fat consumption and modern lifestyle, its conjugation with PHM/NabM-MP complexes may reduce the negative impact of unhealthy food intake and, on some parameters, even improving the whole liver function.
31905423	0	21	theme	biochemical	83:93	arg1	enhancers					95:103	liver biochemical enhancers	77:103	liver biochemical enhancers	77:103	Dietary solutions to dyslipidemia: Milk protein-polysaccharide conjugates as liver biochemical enhancers.
31905423	6	22	theme	functional	1014:1023	arg1	complexes					989:997	These complexes	983:997	These complexes	983:997	These complexes can be used as functional protection elements against the nonalcoholic fatty liver disease.
31905423	6	22	theme	functional	1014:1023	arg1	elements					1036:1043	functional protection elements	1014:1043	functional protection elements against the nonalcoholic fatty liver disease	1014:1088	These complexes can be used as functional protection elements against the nonalcoholic fatty liver disease.
31905423	4	23	from	hyperlipidemia	600:613	arg1	rats					651:654	rats	651:654	rats fed high-fat diet (HFD)	651:678	The effects of resulting complexes on liver function, hyperlipidemia, and histopathology of the liver in rats fed high-fat diet (HFD) were investigated.
31905423	2	24	theme	Nabq	407:410	arg1	NabM					422:425	NabM	422:425	NabM	422:425	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	2	24	theme	Nabq	407:410	arg1	mucilage					412:419	Nabq mucilage	407:419	Nabq mucilage (NabM)	407:426	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	5	25	theme	significant	745:755	arg1	effect					757:762	significant effect	745:762	significant effect	745:762	The results showed that the complexes exerted significant effect on normalizing tested parameters; WPH-NabM had the most significantly decreased level of malondialdehyde content and the liver histopathological examination proved an improvement in all groups fed with these complexes.
31905423	0	26	theme	Dietary	0:6	arg1	solutions					8:16	Dietary solutions	0:16	Dietary solutions to dyslipidemia: Milk protein-polysaccharide conjugates as liver biochemical enhancers.	0:104	Dietary solutions to dyslipidemia: Milk protein-polysaccharide conjugates as liver biochemical enhancers.
31905423	1	27	theme	Protein-polysaccharide	106:127	arg1	focus					154:158	a focus	152:158	a focus of scientific attention	152:182	Protein-polysaccharide interactions have been a focus of scientific attention.
31905423	1	27	theme	Protein-polysaccharide	106:127	arg1	interactions					129:140	Protein-polysaccharide interactions	106:140	Protein-polysaccharide interactions	106:140	Protein-polysaccharide interactions have been a focus of scientific attention.
31905423	4	28	theme	resulting	561:569	arg1	complexes					571:579	resulting complexes	561:579	resulting complexes	561:579	The effects of resulting complexes on liver function, hyperlipidemia, and histopathology of the liver in rats fed high-fat diet (HFD) were investigated.
31905423	7	29	theme	liver	1188:1192	arg1	function					1194:1201	liver function	1188:1201	liver function	1188:1201	PRACTICAL APPLICATIONS: PHM, NabM, and their complexes with milk proteins were proved to improve liver function, enhancing most of its measurable parameters and also diminishing the risk of cardiovascular disease.
31905423	9	30	theme	intake	1627:1632	arg1	impact					1602:1607	the negative impact	1589:1607	the negative impact of unhealthy food intake	1589:1632	Knowing how much these diseases proliferate in the western world and its correlation with high-fat consumption and modern lifestyle, its conjugation with PHM/NabM-MP complexes may reduce the negative impact of unhealthy food intake and, on some parameters, even improving the whole liver function.
31905423	3	31	theme	phenolic	451:458	arg1	content					460:466	phenolic content	451:466	phenolic content	451:466	Chemical composition, phenolic content, and antioxidant activities of milk protein-mucilage complexes were explored.
31905423	4	32	from	effects	550:556	arg1	hyperlipidemia					600:613	hyperlipidemia	600:613	hyperlipidemia	600:613	The effects of resulting complexes on liver function, hyperlipidemia, and histopathology of the liver in rats fed high-fat diet (HFD) were investigated.
31905423	4	32	from	effects	550:556	arg1	histopathology					620:633	histopathology	620:633	histopathology	620:633	The effects of resulting complexes on liver function, hyperlipidemia, and histopathology of the liver in rats fed high-fat diet (HFD) were investigated.
31905423	4	32	from	effects	550:556	arg1	function					590:597	liver function	584:597	liver function	584:597	The effects of resulting complexes on liver function, hyperlipidemia, and histopathology of the liver in rats fed high-fat diet (HFD) were investigated.
31905423	7	33	with	PHM	1115:1117	arg1	proteins					1156:1163	milk proteins	1151:1163	milk proteins	1151:1163	PRACTICAL APPLICATIONS: PHM, NabM, and their complexes with milk proteins were proved to improve liver function, enhancing most of its measurable parameters and also diminishing the risk of cardiovascular disease.
31905423	9	34	with	correlation	1475:1485	arg1	consumption					1501:1511	high-fat consumption	1492:1511	high-fat consumption	1492:1511	Knowing how much these diseases proliferate in the western world and its correlation with high-fat consumption and modern lifestyle, its conjugation with PHM/NabM-MP complexes may reduce the negative impact of unhealthy food intake and, on some parameters, even improving the whole liver function.
31905423	9	34	with	correlation	1475:1485	arg1	lifestyle					1524:1532	modern lifestyle	1517:1532	modern lifestyle	1517:1532	Knowing how much these diseases proliferate in the western world and its correlation with high-fat consumption and modern lifestyle, its conjugation with PHM/NabM-MP complexes may reduce the negative impact of unhealthy food intake and, on some parameters, even improving the whole liver function.
31905423	9	35	with	world	1461:1465	arg1	consumption					1501:1511	high-fat consumption	1492:1511	high-fat consumption	1492:1511	Knowing how much these diseases proliferate in the western world and its correlation with high-fat consumption and modern lifestyle, its conjugation with PHM/NabM-MP complexes may reduce the negative impact of unhealthy food intake and, on some parameters, even improving the whole liver function.
31905423	9	35	with	world	1461:1465	arg1	lifestyle					1524:1532	modern lifestyle	1517:1532	modern lifestyle	1517:1532	Knowing how much these diseases proliferate in the western world and its correlation with high-fat consumption and modern lifestyle, its conjugation with PHM/NabM-MP complexes may reduce the negative impact of unhealthy food intake and, on some parameters, even improving the whole liver function.
31905423	0	36	theme	protein-polysaccharide	40:61	arg1	conjugates					63:72	Milk protein-polysaccharide conjugates	35:72	Dietary solutions to dyslipidemia: Milk protein-polysaccharide conjugates as liver biochemical enhancers.	0:104	Dietary solutions to dyslipidemia: Milk protein-polysaccharide conjugates as liver biochemical enhancers.
31905423	7	37	with	NabM	1120:1123	arg1	proteins					1156:1163	milk proteins	1151:1163	milk proteins	1151:1163	PRACTICAL APPLICATIONS: PHM, NabM, and their complexes with milk proteins were proved to improve liver function, enhancing most of its measurable parameters and also diminishing the risk of cardiovascular disease.
31905423	2	38	theme	milk	275:278	arg1	proteins					280:287	buffalo total milk proteins	261:287	buffalo total milk proteins	261:287	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	2	39	theme	Psyllium	362:369	arg1	PHM					398:400	PHM	398:400	PHM	398:400	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	2	39	theme	Psyllium	362:369	arg1	mucilage					388:395	Psyllium husk (ispaghula) mucilage	362:395	Psyllium husk (ispaghula) mucilage (PHM)	362:401	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	7	40	theme	disease	1296:1302	arg1	risk					1273:1276	the risk	1269:1276	the risk of cardiovascular disease	1269:1302	PRACTICAL APPLICATIONS: PHM, NabM, and their complexes with milk proteins were proved to improve liver function, enhancing most of its measurable parameters and also diminishing the risk of cardiovascular disease.
31905423	6	41	theme	fatty	1070:1074	arg1	disease					1082:1088	the nonalcoholic fatty liver disease	1053:1088	the nonalcoholic fatty liver disease	1053:1088	These complexes can be used as functional protection elements against the nonalcoholic fatty liver disease.
31905423	7	42	with	complexes	1136:1144	arg1	proteins					1156:1163	milk proteins	1151:1163	milk proteins	1151:1163	PRACTICAL APPLICATIONS: PHM, NabM, and their complexes with milk proteins were proved to improve liver function, enhancing most of its measurable parameters and also diminishing the risk of cardiovascular disease.
31905423	7	43	theme	measurable	1226:1235	arg1	parameters					1237:1246	its measurable parameters	1222:1246	its measurable parameters	1222:1246	PRACTICAL APPLICATIONS: PHM, NabM, and their complexes with milk proteins were proved to improve liver function, enhancing most of its measurable parameters and also diminishing the risk of cardiovascular disease.
31905423	6	44	used	used	1006:1009	arg2	complexes					989:997	These complexes	983:997	These complexes	983:997	These complexes can be used as functional protection elements against the nonalcoholic fatty liver disease.
31905423	6	44	used	used	1006:1009	arg2	elements					1036:1043	functional protection elements	1014:1043	functional protection elements against the nonalcoholic fatty liver disease	1014:1088	These complexes can be used as functional protection elements against the nonalcoholic fatty liver disease.
31905423	3	45	theme	protein-mucilage	504:519	arg1	complexes					521:529	milk protein-mucilage complexes	499:529	milk protein-mucilage complexes	499:529	Chemical composition, phenolic content, and antioxidant activities of milk protein-mucilage complexes were explored.
31905423	9	46	theme	whole	1678:1682	arg1	function					1690:1697	the whole liver function	1674:1697	the whole liver function	1674:1697	Knowing how much these diseases proliferate in the western world and its correlation with high-fat consumption and modern lifestyle, its conjugation with PHM/NabM-MP complexes may reduce the negative impact of unhealthy food intake and, on some parameters, even improving the whole liver function.
31905423	9	47	theme	western	1453:1459	arg1	world					1461:1465	the western world	1449:1465	the western world	1449:1465	Knowing how much these diseases proliferate in the western world and its correlation with high-fat consumption and modern lifestyle, its conjugation with PHM/NabM-MP complexes may reduce the negative impact of unhealthy food intake and, on some parameters, even improving the whole liver function.
31905423	11	48	theme	research	2067:2074	arg1	field					2058:2062	a field	2056:2062	a field of research aimed at the better application of milk proteins	2056:2123	Although many interactions concerning milk proteins have already been analyzed, our study also proposes the interaction with bioactive polysaccharides as useful, opening a field of research aimed at the better application of milk proteins.
31905423	2	49	theme	hydrolysate	306:316	arg1	effects					250:256	the antioxidant and hepatoprotective effects	213:256	the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH)	213:322	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	11	50	theme	bioactive	2011:2019	arg1	polysaccharides					2021:2035	bioactive polysaccharides	2011:2035	bioactive polysaccharides	2011:2035	Although many interactions concerning milk proteins have already been analyzed, our study also proposes the interaction with bioactive polysaccharides as useful, opening a field of research aimed at the better application of milk proteins.
31905423	4	51	theme	high-fat	660:667	arg1	diet					669:672	high-fat diet	660:672	high-fat diet (HFD)	660:678	The effects of resulting complexes on liver function, hyperlipidemia, and histopathology of the liver in rats fed high-fat diet (HFD) were investigated.
31905423	4	51	theme	high-fat	660:667	arg1	HFD					675:677	HFD	675:677	HFD	675:677	The effects of resulting complexes on liver function, hyperlipidemia, and histopathology of the liver in rats fed high-fat diet (HFD) were investigated.
31905423	5	52	theme	tested	779:784	arg1	parameters					786:795	tested parameters	779:795	tested parameters	779:795	The results showed that the complexes exerted significant effect on normalizing tested parameters; WPH-NabM had the most significantly decreased level of malondialdehyde content and the liver histopathological examination proved an improvement in all groups fed with these complexes.
31905423	10	53	theme	Nabq	1813:1816	arg1	consumption					1785:1795	consumption	1785:1795	consumption	1785:1795	For that reason, the present study supports and pushes forward the dissemination and consumption of ispaghula or Nabq (the polysaccharides sources) or supplements originating from them.
31905423	10	53	theme	Nabq	1813:1816	arg1	dissemination					1767:1779	dissemination	1767:1779	dissemination	1767:1779	For that reason, the present study supports and pushes forward the dissemination and consumption of ispaghula or Nabq (the polysaccharides sources) or supplements originating from them.
31905423	5	54	theme	liver	885:889	arg1	examination					909:919	the liver histopathological examination	881:919	the liver histopathological examination	881:919	The results showed that the complexes exerted significant effect on normalizing tested parameters; WPH-NabM had the most significantly decreased level of malondialdehyde content and the liver histopathological examination proved an improvement in all groups fed with these complexes.
31905423	11	55	with	interaction	1994:2004	arg1	polysaccharides					2021:2035	bioactive polysaccharides	2011:2035	bioactive polysaccharides	2011:2035	Although many interactions concerning milk proteins have already been analyzed, our study also proposes the interaction with bioactive polysaccharides as useful, opening a field of research aimed at the better application of milk proteins.
31905423	10	56	theme	ispaghula	1800:1808	arg1	consumption					1785:1795	consumption	1785:1795	consumption	1785:1795	For that reason, the present study supports and pushes forward the dissemination and consumption of ispaghula or Nabq (the polysaccharides sources) or supplements originating from them.
31905423	10	56	theme	ispaghula	1800:1808	arg1	dissemination					1767:1779	dissemination	1767:1779	dissemination	1767:1779	For that reason, the present study supports and pushes forward the dissemination and consumption of ispaghula or Nabq (the polysaccharides sources) or supplements originating from them.
31905423	9	57	theme	high-fat	1492:1499	arg1	consumption					1501:1511	high-fat consumption	1492:1511	high-fat consumption	1492:1511	Knowing how much these diseases proliferate in the western world and its correlation with high-fat consumption and modern lifestyle, its conjugation with PHM/NabM-MP complexes may reduce the negative impact of unhealthy food intake and, on some parameters, even improving the whole liver function.
31905423	4	58	theme	liver	642:646	arg1	hyperlipidemia					600:613	hyperlipidemia	600:613	hyperlipidemia	600:613	The effects of resulting complexes on liver function, hyperlipidemia, and histopathology of the liver in rats fed high-fat diet (HFD) were investigated.
31905423	4	58	theme	liver	642:646	arg1	histopathology					620:633	histopathology	620:633	histopathology	620:633	The effects of resulting complexes on liver function, hyperlipidemia, and histopathology of the liver in rats fed high-fat diet (HFD) were investigated.
31905423	4	58	theme	liver	642:646	arg1	function					590:597	liver function	584:597	liver function	584:597	The effects of resulting complexes on liver function, hyperlipidemia, and histopathology of the liver in rats fed high-fat diet (HFD) were investigated.
31905423	10	59	theme	present	1721:1727	arg1	study					1729:1733	the present study	1717:1733	the present study	1717:1733	For that reason, the present study supports and pushes forward the dissemination and consumption of ispaghula or Nabq (the polysaccharides sources) or supplements originating from them.
31905423	7	60	theme	PRACTICAL	1091:1099	arg1	APPLICATIONS					1101:1112	PRACTICAL APPLICATIONS	1091:1112	PRACTICAL APPLICATIONS: PHM, NabM, and their complexes with milk proteins were proved to improve liver function, enhancing most of its measurable parameters and also diminishing the risk of cardiovascular disease.	1091:1303	PRACTICAL APPLICATIONS: PHM, NabM, and their complexes with milk proteins were proved to improve liver function, enhancing most of its measurable parameters and also diminishing the risk of cardiovascular disease.
31905423	2	61	theme	total	269:273	arg1	proteins					280:287	buffalo total milk proteins	261:287	buffalo total milk proteins	261:287	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	5	62	theme	malondialdehyde	853:867	arg1	content					869:875	malondialdehyde content	853:875	malondialdehyde content	853:875	The results showed that the complexes exerted significant effect on normalizing tested parameters; WPH-NabM had the most significantly decreased level of malondialdehyde content and the liver histopathological examination proved an improvement in all groups fed with these complexes.
31905423	5	63	contain	had	807:809	arg1	WPH-NabM					798:805	WPH-NabM	798:805	WPH-NabM	798:805	The results showed that the complexes exerted significant effect on normalizing tested parameters; WPH-NabM had the most significantly decreased level of malondialdehyde content and the liver histopathological examination proved an improvement in all groups fed with these complexes.
31905423	5	63	contain	had	807:809	arg2	level					844:848	the most significantly decreased level	811:848	the most significantly decreased level of malondialdehyde content	811:875	The results showed that the complexes exerted significant effect on normalizing tested parameters; WPH-NabM had the most significantly decreased level of malondialdehyde content and the liver histopathological examination proved an improvement in all groups fed with these complexes.
31905423	2	64	theme	whey	293:296	arg1	WPH					319:321	WPH	319:321	WPH	319:321	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	2	64	theme	whey	293:296	arg1	hydrolysate					306:316	whey protein hydrolysate	293:316	whey protein hydrolysate (WPH)	293:322	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	6	65	theme	nonalcoholic	1057:1068	arg1	disease					1082:1088	the nonalcoholic fatty liver disease	1053:1088	the nonalcoholic fatty liver disease	1053:1088	These complexes can be used as functional protection elements against the nonalcoholic fatty liver disease.
31905423	11	66	theme	milk	2111:2114	arg1	proteins					2116:2123	milk proteins	2111:2123	milk proteins	2111:2123	Although many interactions concerning milk proteins have already been analyzed, our study also proposes the interaction with bioactive polysaccharides as useful, opening a field of research aimed at the better application of milk proteins.
31905423	3	67	theme	antioxidant	473:483	arg1	activities					485:494	antioxidant activities	473:494	antioxidant activities	473:494	Chemical composition, phenolic content, and antioxidant activities of milk protein-mucilage complexes were explored.
31905423	0	68	theme	liver	77:81	arg1	enhancers					95:103	liver biochemical enhancers	77:103	liver biochemical enhancers	77:103	Dietary solutions to dyslipidemia: Milk protein-polysaccharide conjugates as liver biochemical enhancers.
31905423	2	69	theme	husk	371:374	arg1	PHM					398:400	PHM	398:400	PHM	398:400	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	2	69	theme	husk	371:374	arg1	mucilage					388:395	Psyllium husk (ispaghula) mucilage	362:395	Psyllium husk (ispaghula) mucilage (PHM)	362:401	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	2	70	theme	proteins	280:287	arg1	effects					250:256	the antioxidant and hepatoprotective effects	213:256	the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH)	213:322	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	1	71	theme	scientific	163:172	arg1	attention					174:182	scientific attention	163:182	scientific attention	163:182	Protein-polysaccharide interactions have been a focus of scientific attention.
31905423	8	72	theme	health	1335:1340	arg1	circumstances					1342:1354	better health circumstances	1328:1354	better health circumstances	1328:1354	Mice with HFD achieved better health circumstances by combining these ingredients in their diet.
31905423	4	73	from	histopathology	620:633	arg1	rats					651:654	rats	651:654	rats fed high-fat diet (HFD)	651:678	The effects of resulting complexes on liver function, hyperlipidemia, and histopathology of the liver in rats fed high-fat diet (HFD) were investigated.
31905423	6	74	theme	protection	1025:1034	arg1	complexes					989:997	These complexes	983:997	These complexes	983:997	These complexes can be used as functional protection elements against the nonalcoholic fatty liver disease.
31905423	6	74	theme	protection	1025:1034	arg1	elements					1036:1043	functional protection elements	1014:1043	functional protection elements against the nonalcoholic fatty liver disease	1014:1088	These complexes can be used as functional protection elements against the nonalcoholic fatty liver disease.
31905423	5	75	from	improvement	931:941	arg1	groups					950:955	all groups	946:955	all groups fed with these complexes	946:980	The results showed that the complexes exerted significant effect on normalizing tested parameters; WPH-NabM had the most significantly decreased level of malondialdehyde content and the liver histopathological examination proved an improvement in all groups fed with these complexes.
31905423	2	76	with	interactions	344:355	arg1	NabM					422:425	NabM	422:425	NabM	422:425	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	2	76	with	interactions	344:355	arg1	PHM					398:400	PHM	398:400	PHM	398:400	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	2	76	with	interactions	344:355	arg1	mucilage					388:395	Psyllium husk (ispaghula) mucilage	362:395	Psyllium husk (ispaghula) mucilage (PHM)	362:401	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	2	76	with	interactions	344:355	arg1	mucilage					412:419	Nabq mucilage	407:419	Nabq mucilage (NabM)	407:426	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	9	77	theme	negative	1593:1600	arg1	impact					1602:1607	the negative impact	1589:1607	the negative impact of unhealthy food intake	1589:1632	Knowing how much these diseases proliferate in the western world and its correlation with high-fat consumption and modern lifestyle, its conjugation with PHM/NabM-MP complexes may reduce the negative impact of unhealthy food intake and, on some parameters, even improving the whole liver function.
31905423	9	78	with	conjugation	1539:1549	arg1	complexes					1568:1576	PHM/NabM-MP complexes	1556:1576	PHM/NabM-MP complexes	1556:1576	Knowing how much these diseases proliferate in the western world and its correlation with high-fat consumption and modern lifestyle, its conjugation with PHM/NabM-MP complexes may reduce the negative impact of unhealthy food intake and, on some parameters, even improving the whole liver function.
31905423	4	79	theme	complexes	571:579	arg1	effects					550:556	The effects	546:556	The effects of resulting complexes on liver function, hyperlipidemia, and histopathology of the liver in rats fed high-fat diet (HFD)	546:678	The effects of resulting complexes on liver function, hyperlipidemia, and histopathology of the liver in rats fed high-fat diet (HFD) were investigated.
31905423	3	80	theme	Chemical	429:436	arg1	composition					438:448	Chemical composition	429:448	Chemical composition	429:448	Chemical composition, phenolic content, and antioxidant activities of milk protein-mucilage complexes were explored.
31905423	9	81	theme	food	1622:1625	arg1	intake					1627:1632	unhealthy food intake	1612:1632	unhealthy food intake	1612:1632	Knowing how much these diseases proliferate in the western world and its correlation with high-fat consumption and modern lifestyle, its conjugation with PHM/NabM-MP complexes may reduce the negative impact of unhealthy food intake and, on some parameters, even improving the whole liver function.
31905423	4	82	theme	liver	584:588	arg1	function					590:597	liver function	584:597	liver function	584:597	The effects of resulting complexes on liver function, hyperlipidemia, and histopathology of the liver in rats fed high-fat diet (HFD) were investigated.
31905423	0	83	theme	Milk	35:38	arg1	conjugates					63:72	Milk protein-polysaccharide conjugates	35:72	Dietary solutions to dyslipidemia: Milk protein-polysaccharide conjugates as liver biochemical enhancers.	0:104	Dietary solutions to dyslipidemia: Milk protein-polysaccharide conjugates as liver biochemical enhancers.
31905423	6	84	theme	liver	1076:1080	arg1	disease					1082:1088	the nonalcoholic fatty liver disease	1053:1088	the nonalcoholic fatty liver disease	1053:1088	These complexes can be used as functional protection elements against the nonalcoholic fatty liver disease.
31905423	4	85	from	function	590:597	arg1	rats					651:654	rats	651:654	rats fed high-fat diet (HFD)	651:678	The effects of resulting complexes on liver function, hyperlipidemia, and histopathology of the liver in rats fed high-fat diet (HFD) were investigated.
31905423	2	86	theme	hepatoprotective	233:248	arg1	effects					250:256	the antioxidant and hepatoprotective effects	213:256	the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH)	213:322	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	1	87	theme	attention	174:182	arg1	focus					154:158	a focus	152:158	a focus of scientific attention	152:182	Protein-polysaccharide interactions have been a focus of scientific attention.
31905423	1	87	theme	attention	174:182	arg1	interactions					129:140	Protein-polysaccharide interactions	106:140	Protein-polysaccharide interactions	106:140	Protein-polysaccharide interactions have been a focus of scientific attention.
31905423	3	88	theme	milk	499:502	arg1	complexes					521:529	milk protein-mucilage complexes	499:529	milk protein-mucilage complexes	499:529	Chemical composition, phenolic content, and antioxidant activities of milk protein-mucilage complexes were explored.
31905423	10	89	theme	polysaccharides	1823:1837	arg1	ispaghula					1800:1808	ispaghula	1800:1808	ispaghula	1800:1808	For that reason, the present study supports and pushes forward the dissemination and consumption of ispaghula or Nabq (the polysaccharides sources) or supplements originating from them.
31905423	10	89	theme	polysaccharides	1823:1837	arg1	sources					1839:1845	the polysaccharides sources	1819:1845	the polysaccharides sources	1819:1845	For that reason, the present study supports and pushes forward the dissemination and consumption of ispaghula or Nabq (the polysaccharides sources) or supplements originating from them.
31905423	7	90	theme	cardiovascular	1281:1294	arg1	disease					1296:1302	cardiovascular disease	1281:1302	cardiovascular disease	1281:1302	PRACTICAL APPLICATIONS: PHM, NabM, and their complexes with milk proteins were proved to improve liver function, enhancing most of its measurable parameters and also diminishing the risk of cardiovascular disease.
31905423	2	91	theme	antioxidant	217:227	arg1	effects					250:256	the antioxidant and hepatoprotective effects	213:256	the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH)	213:322	This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM).
31905423	5	92	theme	content	869:875	arg1	level					844:848	the most significantly decreased level	811:848	the most significantly decreased level of malondialdehyde content	811:875	The results showed that the complexes exerted significant effect on normalizing tested parameters; WPH-NabM had the most significantly decreased level of malondialdehyde content and the liver histopathological examination proved an improvement in all groups fed with these complexes.
31905423	0	93	dep	solutions	8:16	arg1	conjugates					63:72	Milk protein-polysaccharide conjugates	35:72	Dietary solutions to dyslipidemia: Milk protein-polysaccharide conjugates as liver biochemical enhancers.	0:104	Dietary solutions to dyslipidemia: Milk protein-polysaccharide conjugates as liver biochemical enhancers.
31905423	7	94	dep	APPLICATIONS	1101:1112	arg1	proved					1170:1175	proved	1170:1175	proved	1170:1175	PRACTICAL APPLICATIONS: PHM, NabM, and their complexes with milk proteins were proved to improve liver function, enhancing most of its measurable parameters and also diminishing the risk of cardiovascular disease.
31905423	11	95	theme	milk	1924:1927	arg1	proteins					1929:1936	milk proteins	1924:1936	milk proteins	1924:1936	Although many interactions concerning milk proteins have already been analyzed, our study also proposes the interaction with bioactive polysaccharides as useful, opening a field of research aimed at the better application of milk proteins.
34063701	8	0	theme	groups	1411:1416	arg1	ionization					1385:1394	the ionization	1381:1394	the ionization of hydrophilic groups	1381:1416	The swelling was favored in an alkaline pH due to the ionization of hydrophilic groups.
34063701	5	1	theme	PHP	1029:1031	arg1	incorporation					1033:1045	PHP incorporation	1029:1045	PHP incorporation in the hydrogel	1029:1061	The results showed that PHP incorporation in the hydrogel favored the crosslinking of chains.
34063701	3	2	theme	samples	474:480	arg1	structure					405:413	The structure	401:413	The structure	401:413	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	3	2	theme	samples	474:480	arg1	composition					459:469	chemical composition	450:469	chemical composition	450:469	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	3	2	theme	samples	474:480	arg1	morphology					416:425	morphology	416:425	morphology	416:425	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	3	2	theme	samples	474:480	arg1	stability					436:444	thermal stability	428:444	thermal stability	428:444	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	4	3	theme	kinetics	901:908	arg1	release					876:882	release	876:882	release of nutrients and kinetics	876:908	Swelling/reswelling tests, textural analysis, effect of pH, release of nutrients and kinetics were determined; the ecotoxicity of the hydrogels was investigated by the Artemia salina test.
34063701	4	3	theme	kinetics	901:908	arg1	tests					836:840	Swelling/reswelling tests	816:840	Swelling/reswelling tests	816:840	Swelling/reswelling tests, textural analysis, effect of pH, release of nutrients and kinetics were determined; the ecotoxicity of the hydrogels was investigated by the Artemia salina test.
34063701	9	4	theme	Fickian	1512:1518	arg1	diffusion					1520:1528	Fickian diffusion	1512:1528	Fickian diffusion	1512:1528	The sustained release of phosphorus in HCGP was described by the Korsmeyer-Peppas model, and Fickian diffusion is the main fertilizer release mechanism.
34063701	9	4	theme	Fickian	1512:1518	arg1	mechanism					1561:1569	the main fertilizer release mechanism	1533:1569	the main fertilizer release mechanism	1533:1569	The sustained release of phosphorus in HCGP was described by the Korsmeyer-Peppas model, and Fickian diffusion is the main fertilizer release mechanism.
34063701	1	5	theme	water	167:171	arg1	content					173:179	the water content	163:179	the water content in the soil and availability of fertilizers	163:223	Agricultural production is influenced by the water content in the soil and availability of fertilizers.
34063701	4	6	theme	Artemia	984:990	arg1	test					999:1002	the Artemia salina test	980:1002	the Artemia salina test	980:1002	Swelling/reswelling tests, textural analysis, effect of pH, release of nutrients and kinetics were determined; the ecotoxicity of the hydrogels was investigated by the Artemia salina test.
34063701	9	7	theme	main	1537:1540	arg1	diffusion					1520:1528	Fickian diffusion	1512:1528	Fickian diffusion	1512:1528	The sustained release of phosphorus in HCGP was described by the Korsmeyer-Peppas model, and Fickian diffusion is the main fertilizer release mechanism.
34063701	9	7	theme	main	1537:1540	arg1	mechanism					1561:1569	the main fertilizer release mechanism	1533:1569	the main fertilizer release mechanism	1533:1569	The sustained release of phosphorus in HCGP was described by the Korsmeyer-Peppas model, and Fickian diffusion is the main fertilizer release mechanism.
34063701	2	8	theme	cashew	291:296	arg1	gum					303:305	natural cashew tree gum	283:305	natural cashew tree gum (CG)	283:310	Thus, superabsorbent hydrogels, based on polyacrylamide, natural cashew tree gum (CG) and potassium hydrogen phosphate (PHP), as fertilizer and water releaser were developed.
34063701	2	8	theme	cashew	291:296	arg1	CG					308:309	CG	308:309	CG	308:309	Thus, superabsorbent hydrogels, based on polyacrylamide, natural cashew tree gum (CG) and potassium hydrogen phosphate (PHP), as fertilizer and water releaser were developed.
34063701	6	9	from	stability	1126:1134	arg1	HCGP					1139:1142	HCGP	1139:1142	HCGP	1139:1142	This increased the thermal stability in HCGP but decreased the hardness and adhesion properties.
34063701	0	10	theme	Water	95:99	arg1	Release					84:90	the Controlled Release	69:90	the Controlled Release of Water and Plant Nutrients	69:119	Superabsorbent Hydrogels Based to Polyacrylamide/Cashew Tree Gum for the Controlled Release of Water and Plant Nutrients.
34063701	3	11	theme	Infrared	671:678	arg1	FTIR					694:697	FTIR	694:697	FTIR	694:697	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	3	11	theme	Infrared	671:678	arg1	Spectroscopy					680:691	Fourier Transformed Infrared Spectroscopy	651:691	Fourier Transformed Infrared Spectroscopy (FTIR)	651:698	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	9	12	from	release	1433:1439	arg1	HCGP					1458:1461	HCGP	1458:1461	HCGP	1458:1461	The sustained release of phosphorus in HCGP was described by the Korsmeyer-Peppas model, and Fickian diffusion is the main fertilizer release mechanism.
34063701	2	13	theme	natural	283:289	arg1	gum					303:305	natural cashew tree gum	283:305	natural cashew tree gum (CG)	283:310	Thus, superabsorbent hydrogels, based on polyacrylamide, natural cashew tree gum (CG) and potassium hydrogen phosphate (PHP), as fertilizer and water releaser were developed.
34063701	2	13	theme	natural	283:289	arg1	CG					308:309	CG	308:309	CG	308:309	Thus, superabsorbent hydrogels, based on polyacrylamide, natural cashew tree gum (CG) and potassium hydrogen phosphate (PHP), as fertilizer and water releaser were developed.
34063701	9	14	theme	sustained	1423:1431	arg1	release					1433:1439	The sustained release	1419:1439	The sustained release of phosphorus in HCGP	1419:1461	The sustained release of phosphorus in HCGP was described by the Korsmeyer-Peppas model, and Fickian diffusion is the main fertilizer release mechanism.
34063701	0	15	theme	Nutrients	111:119	arg1	Release					84:90	the Controlled Release	69:90	the Controlled Release of Water and Plant Nutrients	69:119	Superabsorbent Hydrogels Based to Polyacrylamide/Cashew Tree Gum for the Controlled Release of Water and Plant Nutrients.
34063701	10	16	from	application	1651:1661	arg1	agriculture					1666:1676	agriculture	1666:1676	agriculture	1666:1676	Finally, the hydrogels do not demonstrate toxicity, and HCGP has potential for application in agriculture.
34063701	3	17	theme	cashew	504:509	arg1	samples					474:480	samples	474:480	samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG)	474:596	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	0	18	theme	Plant	105:109	arg1	Nutrients					111:119	Plant Nutrients	105:119	Plant Nutrients	105:119	Superabsorbent Hydrogels Based to Polyacrylamide/Cashew Tree Gum for the Controlled Release of Water and Plant Nutrients.
34063701	3	19	theme	Fourier	651:657	arg1	FTIR					694:697	FTIR	694:697	FTIR	694:697	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	3	19	theme	Fourier	651:657	arg1	Spectroscopy					680:691	Fourier Transformed Infrared Spectroscopy	651:691	Fourier Transformed Infrared Spectroscopy (FTIR)	651:698	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	6	20	theme	adhesion	1175:1182	arg1	properties					1184:1193	the hardness and adhesion properties	1158:1193	properties	1184:1193	This increased the thermal stability in HCGP but decreased the hardness and adhesion properties.
34063701	3	21	theme	tree	511:514	arg1	hydrogels					520:528	tree gum hydrogels	511:528	tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG)	511:596	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	9	22	theme	phosphorus	1444:1453	arg1	release					1433:1439	The sustained release	1419:1439	The sustained release of phosphorus in HCGP	1419:1461	The sustained release of phosphorus in HCGP was described by the Korsmeyer-Peppas model, and Fickian diffusion is the main fertilizer release mechanism.
34063701	3	23	theme	Transformed	659:669	arg1	FTIR					694:697	FTIR	694:697	FTIR	694:697	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	3	23	theme	Transformed	659:669	arg1	Spectroscopy					680:691	Fourier Transformed Infrared Spectroscopy	651:691	Fourier Transformed Infrared Spectroscopy (FTIR)	651:698	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	10	24	contain	has	1633:1635	arg2	potential					1637:1645	potential	1637:1645	potential for application in agriculture	1637:1676	Finally, the hydrogels do not demonstrate toxicity, and HCGP has potential for application in agriculture.
34063701	10	24	contain	has	1633:1635	arg1	HCGP					1628:1631	HCGP	1628:1631	HCGP	1628:1631	Finally, the hydrogels do not demonstrate toxicity, and HCGP has potential for application in agriculture.
34063701	0	25	theme	Superabsorbent	0:13	arg1	Hydrogels					15:23	Superabsorbent Hydrogels	0:23	Superabsorbent Hydrogels	0:23	Superabsorbent Hydrogels Based to Polyacrylamide/Cashew Tree Gum for the Controlled Release of Water and Plant Nutrients.
34063701	5	26	theme	chains	1091:1096	arg1	crosslinking					1075:1086	the crosslinking	1071:1086	the crosslinking of chains	1071:1096	The results showed that PHP incorporation in the hydrogel favored the crosslinking of chains.
34063701	6	27	theme	hardness	1162:1169	arg1	properties					1184:1193	the hardness and adhesion properties	1158:1193	properties	1184:1193	This increased the thermal stability in HCGP but decreased the hardness and adhesion properties.
34063701	9	28	theme	Korsmeyer-Peppas	1484:1499	arg1	model					1501:1505	the Korsmeyer-Peppas model	1480:1505	the Korsmeyer-Peppas model	1480:1505	The sustained release of phosphorus in HCGP was described by the Korsmeyer-Peppas model, and Fickian diffusion is the main fertilizer release mechanism.
34063701	9	29	theme	fertilizer	1542:1551	arg1	diffusion					1520:1528	Fickian diffusion	1512:1528	Fickian diffusion	1512:1528	The sustained release of phosphorus in HCGP was described by the Korsmeyer-Peppas model, and Fickian diffusion is the main fertilizer release mechanism.
34063701	9	29	theme	fertilizer	1542:1551	arg1	mechanism					1561:1569	the main fertilizer release mechanism	1533:1569	the main fertilizer release mechanism	1533:1569	The sustained release of phosphorus in HCGP was described by the Korsmeyer-Peppas model, and Fickian diffusion is the main fertilizer release mechanism.
34063701	9	30	theme	release	1553:1559	arg1	diffusion					1520:1528	Fickian diffusion	1512:1528	Fickian diffusion	1512:1528	The sustained release of phosphorus in HCGP was described by the Korsmeyer-Peppas model, and Fickian diffusion is the main fertilizer release mechanism.
34063701	9	30	theme	release	1553:1559	arg1	mechanism					1561:1569	the main fertilizer release mechanism	1533:1569	the main fertilizer release mechanism	1533:1569	The sustained release of phosphorus in HCGP was described by the Korsmeyer-Peppas model, and Fickian diffusion is the main fertilizer release mechanism.
34063701	3	31	dep	polyacrylamide	485:498	arg1	hydrogels					520:528	tree gum hydrogels	511:528	tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG)	511:596	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	7	32	theme	fifty-five	1300:1309	arg1	cycles					1323:1328	fifty-five consecutive cycles	1300:1328	fifty-five consecutive cycles	1300:1328	The HCGP demonstrated good swelling capacity (~15,000 times) and an excellent potential for reuse after fifty-five consecutive cycles.
34063701	3	33	theme	thermal	428:434	arg1	stability					436:444	thermal stability	428:444	thermal stability	428:444	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	4	34	theme	hydrogels	950:958	arg1	ecotoxicity					931:941	the ecotoxicity	927:941	the ecotoxicity of the hydrogels	927:958	Swelling/reswelling tests, textural analysis, effect of pH, release of nutrients and kinetics were determined; the ecotoxicity of the hydrogels was investigated by the Artemia salina test.
34063701	2	35	theme	tree	298:301	arg1	gum					303:305	natural cashew tree gum	283:305	natural cashew tree gum (CG)	283:310	Thus, superabsorbent hydrogels, based on polyacrylamide, natural cashew tree gum (CG) and potassium hydrogen phosphate (PHP), as fertilizer and water releaser were developed.
34063701	2	35	theme	tree	298:301	arg1	CG					308:309	CG	308:309	CG	308:309	Thus, superabsorbent hydrogels, based on polyacrylamide, natural cashew tree gum (CG) and potassium hydrogen phosphate (PHP), as fertilizer and water releaser were developed.
34063701	0	36	theme	Tree	56:59	arg1	Gum					61:63	Polyacrylamide/Cashew Tree Gum	34:63	Polyacrylamide/Cashew Tree Gum for the Controlled Release of Water and Plant Nutrients	34:119	Superabsorbent Hydrogels Based to Polyacrylamide/Cashew Tree Gum for the Controlled Release of Water and Plant Nutrients.
34063701	2	37	theme	potassium	316:324	arg1	PHP					346:348	PHP	346:348	PHP	346:348	Thus, superabsorbent hydrogels, based on polyacrylamide, natural cashew tree gum (CG) and potassium hydrogen phosphate (PHP), as fertilizer and water releaser were developed.
34063701	2	37	theme	potassium	316:324	arg1	phosphate					335:343	potassium hydrogen phosphate	316:343	potassium hydrogen phosphate (PHP)	316:349	Thus, superabsorbent hydrogels, based on polyacrylamide, natural cashew tree gum (CG) and potassium hydrogen phosphate (PHP), as fertilizer and water releaser were developed.
34063701	8	38	theme	alkaline	1362:1369	arg1	pH					1371:1372	an alkaline pH	1359:1372	an alkaline pH due to the ionization of hydrophilic groups	1359:1416	The swelling was favored in an alkaline pH due to the ionization of hydrophilic groups.
34063701	2	39	theme	superabsorbent	232:245	arg1	hydrogels					247:255	superabsorbent hydrogels	232:255	superabsorbent hydrogels	232:255	Thus, superabsorbent hydrogels, based on polyacrylamide, natural cashew tree gum (CG) and potassium hydrogen phosphate (PHP), as fertilizer and water releaser were developed.
34063701	7	40	theme	swelling	1223:1230	arg1	capacity					1232:1239	good swelling capacity	1218:1239	good swelling capacity (~15,000 times)	1218:1255	The HCGP demonstrated good swelling capacity (~15,000 times) and an excellent potential for reuse after fifty-five consecutive cycles.
34063701	7	40	theme	swelling	1223:1230	arg1	times					1250:1254	~15,000 times	1242:1254	~15,000 times	1242:1254	The HCGP demonstrated good swelling capacity (~15,000 times) and an excellent potential for reuse after fifty-five consecutive cycles.
34063701	7	41	theme	good	1218:1221	arg1	capacity					1232:1239	good swelling capacity	1218:1239	good swelling capacity (~15,000 times)	1218:1255	The HCGP demonstrated good swelling capacity (~15,000 times) and an excellent potential for reuse after fifty-five consecutive cycles.
34063701	7	41	theme	good	1218:1221	arg1	times					1250:1254	~15,000 times	1242:1254	~15,000 times	1242:1254	The HCGP demonstrated good swelling capacity (~15,000 times) and an excellent potential for reuse after fifty-five consecutive cycles.
34063701	0	42	theme	Polyacrylamide/Cashew	34:54	arg1	Gum					61:63	Polyacrylamide/Cashew Tree Gum	34:63	Polyacrylamide/Cashew Tree Gum for the Controlled Release of Water and Plant Nutrients	34:119	Superabsorbent Hydrogels Based to Polyacrylamide/Cashew Tree Gum for the Controlled Release of Water and Plant Nutrients.
34063701	1	43	dep	soil	188:191	arg1	the					184:186	the	184:186	the	184:186	Agricultural production is influenced by the water content in the soil and availability of fertilizers.
34063701	1	44	theme	fertilizers	213:223	arg1	availability					197:208	availability	197:208	availability	197:208	Agricultural production is influenced by the water content in the soil and availability of fertilizers.
34063701	1	44	theme	fertilizers	213:223	arg1	soil					188:191	soil	188:191	soil	188:191	Agricultural production is influenced by the water content in the soil and availability of fertilizers.
34063701	5	45	from	incorporation	1033:1045	arg1	hydrogel					1054:1061	the hydrogel	1050:1061	the hydrogel	1050:1061	The results showed that PHP incorporation in the hydrogel favored the crosslinking of chains.
34063701	3	46	theme	fertilizer	551:560	arg1	presence					539:546	the presence	535:546	the presence of fertilizer (HCGP)	535:567	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	2	47	theme	hydrogen	326:333	arg1	PHP					346:348	PHP	346:348	PHP	346:348	Thus, superabsorbent hydrogels, based on polyacrylamide, natural cashew tree gum (CG) and potassium hydrogen phosphate (PHP), as fertilizer and water releaser were developed.
34063701	2	47	theme	hydrogen	326:333	arg1	phosphate					335:343	potassium hydrogen phosphate	316:343	potassium hydrogen phosphate (PHP)	316:349	Thus, superabsorbent hydrogels, based on polyacrylamide, natural cashew tree gum (CG) and potassium hydrogen phosphate (PHP), as fertilizer and water releaser were developed.
34063701	3	48	with	hydrogels	520:528	arg1	presence					539:546	the presence	535:546	the presence of fertilizer (HCGP)	535:567	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	3	49	theme	Scanning	701:708	arg1	SEM					731:733	SEM	731:733	SEM	731:733	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	3	49	theme	Scanning	701:708	arg1	Microscopy					719:728	Scanning Electron Microscopy	701:728	Scanning Electron Microscopy (SEM)	701:734	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	4	50	theme	Swelling/reswelling	816:834	arg1	release					876:882	release	876:882	release of nutrients and kinetics	876:908	Swelling/reswelling tests, textural analysis, effect of pH, release of nutrients and kinetics were determined; the ecotoxicity of the hydrogels was investigated by the Artemia salina test.
34063701	4	50	theme	Swelling/reswelling	816:834	arg1	effect					862:867	effect	862:867	effect of pH	862:873	Swelling/reswelling tests, textural analysis, effect of pH, release of nutrients and kinetics were determined; the ecotoxicity of the hydrogels was investigated by the Artemia salina test.
34063701	4	50	theme	Swelling/reswelling	816:834	arg1	tests					836:840	Swelling/reswelling tests	816:840	Swelling/reswelling tests	816:840	Swelling/reswelling tests, textural analysis, effect of pH, release of nutrients and kinetics were determined; the ecotoxicity of the hydrogels was investigated by the Artemia salina test.
34063701	4	50	theme	Swelling/reswelling	816:834	arg1	analysis					852:859	textural analysis	843:859	textural analysis	843:859	Swelling/reswelling tests, textural analysis, effect of pH, release of nutrients and kinetics were determined; the ecotoxicity of the hydrogels was investigated by the Artemia salina test.
34063701	3	51	theme	X-ray	623:627	arg1	XRD					645:647	XRD	645:647	XRD	645:647	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	3	51	theme	X-ray	623:627	arg1	diffractometry					629:642	X-ray diffractometry	623:642	X-ray diffractometry (XRD)	623:648	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	1	52	theme	Agricultural	122:133	arg1	production					135:144	Agricultural production	122:144	Agricultural production	122:144	Agricultural production is influenced by the water content in the soil and availability of fertilizers.
34063701	3	53	theme	Dispersive	785:794	arg1	EDS					810:812	EDS	810:812	EDS	810:812	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	3	53	theme	Dispersive	785:794	arg1	Spectroscopy					796:807	Energy Dispersive Spectroscopy	778:807	Energy Dispersive Spectroscopy (EDS)	778:813	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	3	54	theme	Electron	710:717	arg1	SEM					731:733	SEM	731:733	SEM	731:733	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	3	54	theme	Electron	710:717	arg1	Microscopy					719:728	Scanning Electron Microscopy	701:728	Scanning Electron Microscopy (SEM)	701:734	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	4	55	theme	textural	843:850	arg1	tests					836:840	Swelling/reswelling tests	816:840	Swelling/reswelling tests	816:840	Swelling/reswelling tests, textural analysis, effect of pH, release of nutrients and kinetics were determined; the ecotoxicity of the hydrogels was investigated by the Artemia salina test.
34063701	4	55	theme	textural	843:850	arg1	analysis					852:859	textural analysis	843:859	textural analysis	843:859	Swelling/reswelling tests, textural analysis, effect of pH, release of nutrients and kinetics were determined; the ecotoxicity of the hydrogels was investigated by the Artemia salina test.
34063701	7	56	theme	consecutive	1311:1321	arg1	cycles					1323:1328	fifty-five consecutive cycles	1300:1328	fifty-five consecutive cycles	1300:1328	The HCGP demonstrated good swelling capacity (~15,000 times) and an excellent potential for reuse after fifty-five consecutive cycles.
34063701	4	57	theme	salina	992:997	arg1	test					999:1002	the Artemia salina test	980:1002	the Artemia salina test	980:1002	Swelling/reswelling tests, textural analysis, effect of pH, release of nutrients and kinetics were determined; the ecotoxicity of the hydrogels was investigated by the Artemia salina test.
34063701	8	58	theme	hydrophilic	1399:1409	arg1	groups					1411:1416	hydrophilic groups	1399:1416	hydrophilic groups	1399:1416	The swelling was favored in an alkaline pH due to the ionization of hydrophilic groups.
34063701	3	59	theme	gum	516:518	arg1	hydrogels					520:528	tree gum hydrogels	511:528	tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG)	511:596	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	3	60	theme	chemical	450:457	arg1	composition					459:469	chemical composition	450:469	chemical composition	450:469	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	3	61	theme	Thermogravimetric	737:753	arg1	TGA/DTG					765:771	TGA/DTG	765:771	TGA/DTG	765:771	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	3	61	theme	Thermogravimetric	737:753	arg1	Analysis					755:762	Thermogravimetric Analysis	737:762	Thermogravimetric Analysis (TGA/DTG)	737:772	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	0	62	theme	Controlled	73:82	arg1	Release					84:90	the Controlled Release	69:90	the Controlled Release of Water and Plant Nutrients	69:119	Superabsorbent Hydrogels Based to Polyacrylamide/Cashew Tree Gum for the Controlled Release of Water and Plant Nutrients.
34063701	2	63	theme	water	370:374	arg1	releaser					376:383	water releaser	370:383	water releaser	370:383	Thus, superabsorbent hydrogels, based on polyacrylamide, natural cashew tree gum (CG) and potassium hydrogen phosphate (PHP), as fertilizer and water releaser were developed.
34063701	6	64	theme	thermal	1118:1124	arg1	stability					1126:1134	the thermal stability	1114:1134	the thermal stability in HCGP	1114:1142	This increased the thermal stability in HCGP but decreased the hardness and adhesion properties.
34063701	3	65	theme	polyacrylamide	485:498	arg1	samples					474:480	samples	474:480	samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG)	474:596	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	3	66	theme	Energy	778:783	arg1	EDS					810:812	EDS	810:812	EDS	810:812	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	3	66	theme	Energy	778:783	arg1	Spectroscopy					796:807	Energy Dispersive Spectroscopy	778:807	Energy Dispersive Spectroscopy (EDS)	778:813	The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS).
34063701	4	67	theme	nutrients	887:895	arg1	release					876:882	release	876:882	release of nutrients and kinetics	876:908	Swelling/reswelling tests, textural analysis, effect of pH, release of nutrients and kinetics were determined; the ecotoxicity of the hydrogels was investigated by the Artemia salina test.
34063701	4	67	theme	nutrients	887:895	arg1	tests					836:840	Swelling/reswelling tests	816:840	Swelling/reswelling tests	816:840	Swelling/reswelling tests, textural analysis, effect of pH, release of nutrients and kinetics were determined; the ecotoxicity of the hydrogels was investigated by the Artemia salina test.
34063701	7	68	theme	excellent	1264:1272	arg1	potential					1274:1282	an excellent potential	1261:1282	an excellent potential for reuse after fifty-five consecutive cycles	1261:1328	The HCGP demonstrated good swelling capacity (~15,000 times) and an excellent potential for reuse after fifty-five consecutive cycles.
34063701	1	69	from	content	173:179	arg1	availability					197:208	availability	197:208	availability	197:208	Agricultural production is influenced by the water content in the soil and availability of fertilizers.
34063701	1	69	from	content	173:179	arg1	soil					188:191	soil	188:191	soil	188:191	Agricultural production is influenced by the water content in the soil and availability of fertilizers.
34063701	4	70	theme	pH	872:873	arg1	effect					862:867	effect	862:867	effect of pH	862:873	Swelling/reswelling tests, textural analysis, effect of pH, release of nutrients and kinetics were determined; the ecotoxicity of the hydrogels was investigated by the Artemia salina test.
34063701	4	70	theme	pH	872:873	arg1	tests					836:840	Swelling/reswelling tests	816:840	Swelling/reswelling tests	816:840	Swelling/reswelling tests, textural analysis, effect of pH, release of nutrients and kinetics were determined; the ecotoxicity of the hydrogels was investigated by the Artemia salina test.
33401254	2	0	theme	constant	360:367	arg1	advantage					323:331	advantage	323:331	advantage of the improved dielectric constant	323:367	By taking advantage of the improved dielectric constant, the nanosheets/BC composites were employed as capacitive sensors.
33401254	6	1	theme	low-cost	1019:1026	arg1	electronics					1055:1065	biocompatible, low-cost, and eco-friendly wearable electronics	1004:1065	biocompatible, low-cost, and eco-friendly wearable electronics	1004:1065	The strategy for enhancing the dielectric properties as well as sensing performances of the BC demonstrated herein will be essential for the future development of biocompatible, low-cost, and eco-friendly wearable electronics.
33401254	6	2	theme	dielectric	872:881	arg1	properties					883:892	the dielectric properties	868:892	the dielectric properties as well as sensing performances of the BC demonstrated herein	868:954	The strategy for enhancing the dielectric properties as well as sensing performances of the BC demonstrated herein will be essential for the future development of biocompatible, low-cost, and eco-friendly wearable electronics.
33401254	2	3	theme	dielectric	349:358	arg1	constant					360:367	the improved dielectric constant	336:367	the improved dielectric constant	336:367	By taking advantage of the improved dielectric constant, the nanosheets/BC composites were employed as capacitive sensors.
33401254	6	4	theme	biocompatible	1004:1016	arg1	electronics					1055:1065	biocompatible, low-cost, and eco-friendly wearable electronics	1004:1065	biocompatible, low-cost, and eco-friendly wearable electronics	1004:1065	The strategy for enhancing the dielectric properties as well as sensing performances of the BC demonstrated herein will be essential for the future development of biocompatible, low-cost, and eco-friendly wearable electronics.
33401254	4	5	theme	sensor	650:655	arg1	operation					613:621	Stable operation	606:621	Stable operation	606:621	Stable operation and high robustness of the sensor were demonstrated, where simple human motions could be efficiently monitored.
33401254	4	5	theme	sensor	650:655	arg1	robustness					632:641	high robustness	627:641	high robustness	627:641	Stable operation and high robustness of the sensor were demonstrated, where simple human motions could be efficiently monitored.
33401254	2	6	theme	improved	340:347	arg1	constant					360:367	the improved dielectric constant	336:367	the improved dielectric constant	336:367	By taking advantage of the improved dielectric constant, the nanosheets/BC composites were employed as capacitive sensors.
33401254	5	7	theme	BC	799:800	arg1	paper					812:816	flexible and low-cost BC composite paper	777:816	flexible and low-cost BC composite paper	777:816	This study provided a route for preparing flexible and low-cost BC composite paper for capacitive sensor.
33401254	3	8	from	N	533:533	arg1	performance					486:496	the highest sensing performance	466:496	the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N	466:533	The fabricated devices showed the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N when incorporating as little as 3 vol% of Ti0.91O2 NSs (or ∼2 wt% Ti).
33401254	6	9	theme	wearable	1046:1053	arg1	electronics					1055:1065	biocompatible, low-cost, and eco-friendly wearable electronics	1004:1065	biocompatible, low-cost, and eco-friendly wearable electronics	1004:1065	The strategy for enhancing the dielectric properties as well as sensing performances of the BC demonstrated herein will be essential for the future development of biocompatible, low-cost, and eco-friendly wearable electronics.
33401254	3	10	theme	fabricated	440:449	arg1	devices					451:457	The fabricated devices	436:457	The fabricated devices	436:457	The fabricated devices showed the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N when incorporating as little as 3 vol% of Ti0.91O2 NSs (or ∼2 wt% Ti).
33401254	6	11	theme	future	982:987	arg1	development					989:999	the future development	978:999	the future development of biocompatible, low-cost, and eco-friendly wearable electronics	978:1065	The strategy for enhancing the dielectric properties as well as sensing performances of the BC demonstrated herein will be essential for the future development of biocompatible, low-cost, and eco-friendly wearable electronics.
33401254	3	12	theme	kPa-1	514:518	arg1	performance					486:496	the highest sensing performance	466:496	the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N	466:533	The fabricated devices showed the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N when incorporating as little as 3 vol% of Ti0.91O2 NSs (or ∼2 wt% Ti).
33401254	5	13	theme	capacitive	822:831	arg1	sensor					833:838	capacitive sensor	822:838	capacitive sensor	822:838	This study provided a route for preparing flexible and low-cost BC composite paper for capacitive sensor.
33401254	0	14	theme	capacitive	9:18	arg1	sensor					20:25	Flexible capacitive sensor	0:25	Flexible capacitive sensor	0:25	Flexible capacitive sensor based on 2D-titanium dioxide nanosheets/bacterial cellulose composite film.
33401254	6	15	theme	electronics	1055:1065	arg1	development					989:999	the future development	978:999	the future development of biocompatible, low-cost, and eco-friendly wearable electronics	978:1065	The strategy for enhancing the dielectric properties as well as sensing performances of the BC demonstrated herein will be essential for the future development of biocompatible, low-cost, and eco-friendly wearable electronics.
33401254	1	16	theme	bacterial	184:192	arg1	film					209:212	bacterial cellulose (BC) film	184:212	bacterial cellulose (BC) film for dielectric property tuning	184:243	In this paper, titanium dioxide nanosheets (Ti0.91O2 NSs) were incorporated into bacterial cellulose (BC) film for dielectric property tuning while maintaining the flexibility of the resulting composite paper.
33401254	0	17	theme	Flexible	0:7	arg1	sensor					20:25	Flexible capacitive sensor	0:25	Flexible capacitive sensor	0:25	Flexible capacitive sensor based on 2D-titanium dioxide nanosheets/bacterial cellulose composite film.
33401254	4	18	theme	human	689:693	arg1	motions					695:701	simple human motions	682:701	simple human motions	682:701	Stable operation and high robustness of the sensor were demonstrated, where simple human motions could be efficiently monitored.
33401254	2	19	theme	nanosheets/BC	374:386	arg1	sensors					427:433	capacitive sensors	416:433	capacitive sensors	416:433	By taking advantage of the improved dielectric constant, the nanosheets/BC composites were employed as capacitive sensors.
33401254	2	19	theme	nanosheets/BC	374:386	arg1	composites					388:397	the nanosheets/BC composites	370:397	the nanosheets/BC composites	370:397	By taking advantage of the improved dielectric constant, the nanosheets/BC composites were employed as capacitive sensors.
33401254	5	20	theme	composite	802:810	arg1	paper					812:816	flexible and low-cost BC composite paper	777:816	flexible and low-cost BC composite paper	777:816	This study provided a route for preparing flexible and low-cost BC composite paper for capacitive sensor.
33401254	6	21	theme	BC	933:934	arg1	performances					913:924	sensing performances	905:924	the dielectric properties as well as sensing performances of the BC demonstrated herein	868:954	The strategy for enhancing the dielectric properties as well as sensing performances of the BC demonstrated herein will be essential for the future development of biocompatible, low-cost, and eco-friendly wearable electronics.
33401254	6	21	theme	BC	933:934	arg1	properties					883:892	the dielectric properties	868:892	the dielectric properties as well as sensing performances of the BC demonstrated herein	868:954	The strategy for enhancing the dielectric properties as well as sensing performances of the BC demonstrated herein will be essential for the future development of biocompatible, low-cost, and eco-friendly wearable electronics.
33401254	3	22	dep	30	530:531	arg1	to					527:528	to	527:528	to	527:528	The fabricated devices showed the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N when incorporating as little as 3 vol% of Ti0.91O2 NSs (or ∼2 wt% Ti).
33401254	3	23	theme	sensing	478:484	arg1	performance					486:496	the highest sensing performance	466:496	the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N	466:533	The fabricated devices showed the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N when incorporating as little as 3 vol% of Ti0.91O2 NSs (or ∼2 wt% Ti).
33401254	1	24	theme	resulting	286:294	arg1	paper					306:310	the resulting composite paper	282:310	the resulting composite paper	282:310	In this paper, titanium dioxide nanosheets (Ti0.91O2 NSs) were incorporated into bacterial cellulose (BC) film for dielectric property tuning while maintaining the flexibility of the resulting composite paper.
33401254	3	25	theme	3	567:567	arg1	vol					569:571	vol	569:571	vol	569:571	The fabricated devices showed the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N when incorporating as little as 3 vol% of Ti0.91O2 NSs (or ∼2 wt% Ti).
33401254	1	26	theme	BC	205:206	arg1	film					209:212	bacterial cellulose (BC) film	184:212	bacterial cellulose (BC) film for dielectric property tuning	184:243	In this paper, titanium dioxide nanosheets (Ti0.91O2 NSs) were incorporated into bacterial cellulose (BC) film for dielectric property tuning while maintaining the flexibility of the resulting composite paper.
33401254	0	27	theme	2D-titanium	36:46	arg1	film					97:100	2D-titanium dioxide nanosheets/bacterial cellulose composite film	36:100	2D-titanium dioxide nanosheets/bacterial cellulose composite film	36:100	Flexible capacitive sensor based on 2D-titanium dioxide nanosheets/bacterial cellulose composite film.
33401254	1	28	theme	composite	296:304	arg1	paper					306:310	the resulting composite paper	282:310	the resulting composite paper	282:310	In this paper, titanium dioxide nanosheets (Ti0.91O2 NSs) were incorporated into bacterial cellulose (BC) film for dielectric property tuning while maintaining the flexibility of the resulting composite paper.
33401254	4	29	theme	Stable	606:611	arg1	operation					613:621	Stable operation	606:621	Stable operation	606:621	Stable operation and high robustness of the sensor were demonstrated, where simple human motions could be efficiently monitored.
33401254	3	30	theme	NSs	586:588	arg1	NSs					586:588	Ti0.91O2 NSs	577:588	Ti0.91O2 NSs (or ∼2 wt% Ti)	577:603	The fabricated devices showed the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N when incorporating as little as 3 vol% of Ti0.91O2 NSs (or ∼2 wt% Ti).
33401254	3	30	theme	NSs	586:588	arg1	Ti					601:602	∼2 wt% Ti	594:602	∼2 wt% Ti	594:602	The fabricated devices showed the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N when incorporating as little as 3 vol% of Ti0.91O2 NSs (or ∼2 wt% Ti).
33401254	3	30	theme	NSs	586:588	arg1	%					572:572	3 vol%	567:572	3 vol% of Ti0.91O2 NSs (or ∼2 wt% Ti)	567:603	The fabricated devices showed the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N when incorporating as little as 3 vol% of Ti0.91O2 NSs (or ∼2 wt% Ti).
33401254	1	31	theme	cellulose	194:202	arg1	film					209:212	bacterial cellulose (BC) film	184:212	bacterial cellulose (BC) film for dielectric property tuning	184:243	In this paper, titanium dioxide nanosheets (Ti0.91O2 NSs) were incorporated into bacterial cellulose (BC) film for dielectric property tuning while maintaining the flexibility of the resulting composite paper.
33401254	2	32	theme	capacitive	416:425	arg1	sensors					427:433	capacitive sensors	416:433	capacitive sensors	416:433	By taking advantage of the improved dielectric constant, the nanosheets/BC composites were employed as capacitive sensors.
33401254	2	32	theme	capacitive	416:425	arg1	composites					388:397	the nanosheets/BC composites	370:397	the nanosheets/BC composites	370:397	By taking advantage of the improved dielectric constant, the nanosheets/BC composites were employed as capacitive sensors.
33401254	1	33	theme	paper	306:310	arg1	flexibility					267:277	the flexibility	263:277	the flexibility of the resulting composite paper	263:310	In this paper, titanium dioxide nanosheets (Ti0.91O2 NSs) were incorporated into bacterial cellulose (BC) film for dielectric property tuning while maintaining the flexibility of the resulting composite paper.
33401254	5	34	theme	flexible	777:784	arg1	paper					812:816	flexible and low-cost BC composite paper	777:816	flexible and low-cost BC composite paper	777:816	This study provided a route for preparing flexible and low-cost BC composite paper for capacitive sensor.
33401254	3	35	theme	highest	470:476	arg1	performance					486:496	the highest sensing performance	466:496	the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N	466:533	The fabricated devices showed the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N when incorporating as little as 3 vol% of Ti0.91O2 NSs (or ∼2 wt% Ti).
33401254	0	36	theme	nanosheets/bacterial	56:75	arg1	film					97:100	2D-titanium dioxide nanosheets/bacterial cellulose composite film	36:100	2D-titanium dioxide nanosheets/bacterial cellulose composite film	36:100	Flexible capacitive sensor based on 2D-titanium dioxide nanosheets/bacterial cellulose composite film.
33401254	6	37	theme	sensing	905:911	arg1	performances					913:924	sensing performances	905:924	the dielectric properties as well as sensing performances of the BC demonstrated herein	868:954	The strategy for enhancing the dielectric properties as well as sensing performances of the BC demonstrated herein will be essential for the future development of biocompatible, low-cost, and eco-friendly wearable electronics.
33401254	1	38	theme	titanium	118:125	arg1	dioxide					127:133	titanium dioxide	118:133	titanium dioxide nanosheets (Ti0.91O2 NSs)	118:159	In this paper, titanium dioxide nanosheets (Ti0.91O2 NSs) were incorporated into bacterial cellulose (BC) film for dielectric property tuning while maintaining the flexibility of the resulting composite paper.
33401254	0	39	theme	dioxide	48:54	arg1	film					97:100	2D-titanium dioxide nanosheets/bacterial cellulose composite film	36:100	2D-titanium dioxide nanosheets/bacterial cellulose composite film	36:100	Flexible capacitive sensor based on 2D-titanium dioxide nanosheets/bacterial cellulose composite film.
33401254	3	40	theme	Ti0.91O2	577:584	arg1	NSs					586:588	Ti0.91O2 NSs	577:588	Ti0.91O2 NSs (or ∼2 wt% Ti)	577:603	The fabricated devices showed the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N when incorporating as little as 3 vol% of Ti0.91O2 NSs (or ∼2 wt% Ti).
33401254	3	40	theme	Ti0.91O2	577:584	arg1	Ti					601:602	∼2 wt% Ti	594:602	∼2 wt% Ti	594:602	The fabricated devices showed the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N when incorporating as little as 3 vol% of Ti0.91O2 NSs (or ∼2 wt% Ti).
33401254	1	41	theme	dioxide	127:133	arg1	nanosheets					135:144	titanium dioxide nanosheets	118:144	titanium dioxide nanosheets (Ti0.91O2 NSs)	118:159	In this paper, titanium dioxide nanosheets (Ti0.91O2 NSs) were incorporated into bacterial cellulose (BC) film for dielectric property tuning while maintaining the flexibility of the resulting composite paper.
33401254	1	41	theme	dioxide	127:133	arg1	NSs					156:158	NSs	156:158	NSs	156:158	In this paper, titanium dioxide nanosheets (Ti0.91O2 NSs) were incorporated into bacterial cellulose (BC) film for dielectric property tuning while maintaining the flexibility of the resulting composite paper.
33401254	4	42	theme	high	627:630	arg1	robustness					632:641	high robustness	627:641	high robustness	627:641	Stable operation and high robustness of the sensor were demonstrated, where simple human motions could be efficiently monitored.
33401254	3	43	theme	wt	597:598	arg1	NSs					586:588	Ti0.91O2 NSs	577:588	Ti0.91O2 NSs (or ∼2 wt% Ti)	577:603	The fabricated devices showed the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N when incorporating as little as 3 vol% of Ti0.91O2 NSs (or ∼2 wt% Ti).
33401254	3	43	theme	wt	597:598	arg1	Ti					601:602	∼2 wt% Ti	594:602	∼2 wt% Ti	594:602	The fabricated devices showed the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N when incorporating as little as 3 vol% of Ti0.91O2 NSs (or ∼2 wt% Ti).
33401254	4	44	theme	simple	682:687	arg1	motions					695:701	simple human motions	682:701	simple human motions	682:701	Stable operation and high robustness of the sensor were demonstrated, where simple human motions could be efficiently monitored.
33401254	3	45	theme	%	599:599	arg1	NSs					586:588	Ti0.91O2 NSs	577:588	Ti0.91O2 NSs (or ∼2 wt% Ti)	577:603	The fabricated devices showed the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N when incorporating as little as 3 vol% of Ti0.91O2 NSs (or ∼2 wt% Ti).
33401254	3	45	theme	%	599:599	arg1	Ti					601:602	∼2 wt% Ti	594:602	∼2 wt% Ti	594:602	The fabricated devices showed the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N when incorporating as little as 3 vol% of Ti0.91O2 NSs (or ∼2 wt% Ti).
33401254	3	46	theme	∼2.44 × 10-3	501:512	arg1	kPa-1					514:518	∼2.44 × 10-3 kPa-1	501:518	∼2.44 × 10-3 kPa-1	501:518	The fabricated devices showed the highest sensing performance of ∼2.44 × 10-3 kPa-1 from 0 to 30 N when incorporating as little as 3 vol% of Ti0.91O2 NSs (or ∼2 wt% Ti).
33401254	1	47	theme	dielectric	218:227	arg1	tuning					238:243	dielectric property tuning	218:243	dielectric property tuning	218:243	In this paper, titanium dioxide nanosheets (Ti0.91O2 NSs) were incorporated into bacterial cellulose (BC) film for dielectric property tuning while maintaining the flexibility of the resulting composite paper.
33401254	0	48	theme	composite	87:95	arg1	film					97:100	2D-titanium dioxide nanosheets/bacterial cellulose composite film	36:100	2D-titanium dioxide nanosheets/bacterial cellulose composite film	36:100	Flexible capacitive sensor based on 2D-titanium dioxide nanosheets/bacterial cellulose composite film.
33401254	5	49	theme	low-cost	790:797	arg1	paper					812:816	flexible and low-cost BC composite paper	777:816	flexible and low-cost BC composite paper	777:816	This study provided a route for preparing flexible and low-cost BC composite paper for capacitive sensor.
33401254	1	50	theme	property	229:236	arg1	tuning					238:243	dielectric property tuning	218:243	dielectric property tuning	218:243	In this paper, titanium dioxide nanosheets (Ti0.91O2 NSs) were incorporated into bacterial cellulose (BC) film for dielectric property tuning while maintaining the flexibility of the resulting composite paper.
33401254	0	51	theme	cellulose	77:85	arg1	film					97:100	2D-titanium dioxide nanosheets/bacterial cellulose composite film	36:100	2D-titanium dioxide nanosheets/bacterial cellulose composite film	36:100	Flexible capacitive sensor based on 2D-titanium dioxide nanosheets/bacterial cellulose composite film.
33332094	10	0	theme	Rheological	1554:1564	arg1	properties					1566:1575	Rheological properties	1554:1575	Rheological properties of the hydrogels	1554:1592	Rheological properties of the hydrogels were characterized, and a reversible thermal transition was found for DG-XG/CNC gels at 35 °C.
33332094	7	1	dep	DG-XG	1102:1106	arg1	a					1100:1100	a	1100:1100	a	1100:1100	Gelation occurred at a DG-XG or XGu to CNC ratio higher than that needed for the full surface coverage of CNCs and required relatively high overall concentrations of both components (tested concentrations up to 20 g/L XG and 30 g/L CNCs).
33332094	4	2	theme	molar	712:716	arg1	mass					718:721	comparable molar mass	701:721	comparable molar mass	701:721	XG with native composition and comparable molar mass to DG-XG was produced by an ultrasonication treatment (XGu) for a direct comparison of behavior.
33332094	7	3	dep	XG	1297:1298	arg1	up					1284:1285	up	1284:1285	up	1284:1285	Gelation occurred at a DG-XG or XGu to CNC ratio higher than that needed for the full surface coverage of CNCs and required relatively high overall concentrations of both components (tested concentrations up to 20 g/L XG and 30 g/L CNCs).
33332094	8	4	from	result	1335:1340	arg1	volume					1384:1389	effective hydrodynamic volume	1361:1389	effective hydrodynamic volume of CNCs due to the formation of XG-CNC complexes	1361:1438	This is likely a result of the increase in effective hydrodynamic volume of CNCs due to the formation of XG-CNC complexes.
33332094	4	5	theme	comparable	701:710	arg1	mass					718:721	comparable molar mass	701:721	comparable molar mass	701:721	XG with native composition and comparable molar mass to DG-XG was produced by an ultrasonication treatment (XGu) for a direct comparison of behavior.
33332094	10	6	theme	hydrogels	1584:1592	arg1	properties					1566:1575	Rheological properties	1554:1575	Rheological properties of the hydrogels	1554:1592	Rheological properties of the hydrogels were characterized, and a reversible thermal transition was found for DG-XG/CNC gels at 35 °C.
33332094	6	7	theme	XGu	968:970	arg1	ratios					949:954	the ratios	945:954	the ratios of DG-XG or XGu to CNCs that yielded a viscous liquid, a phase-separated mixture, a simple gel, or a thermoresponsive gel	945:1076	Phase diagrams were established to identify the ratios of DG-XG or XGu to CNCs that yielded a viscous liquid, a phase-separated mixture, a simple gel, or a thermoresponsive gel.
33332094	7	8	theme	higher	1128:1133	arg1	ratio					1122:1126	CNC ratio	1118:1126	CNC ratio higher than that needed for the full surface coverage of CNCs	1118:1188	Gelation occurred at a DG-XG or XGu to CNC ratio higher than that needed for the full surface coverage of CNCs and required relatively high overall concentrations of both components (tested concentrations up to 20 g/L XG and 30 g/L CNCs).
33332094	8	9	theme	due	1399:1401	arg1	CNCs					1394:1397	CNCs	1394:1397	CNCs due to the formation of XG-CNC complexes	1394:1438	This is likely a result of the increase in effective hydrodynamic volume of CNCs due to the formation of XG-CNC complexes.
33332094	5	10	from	CNCs	886:889	arg1	water					894:898	water	894:898	water	894:898	The hydrogels were prepared by simple mixing of DG-XG or XGu with CNCs in water.
33332094	5	11	theme	XGu	877:879	arg1	mixing					858:863	simple mixing	851:863	simple mixing of DG-XG or XGu with CNCs in water	851:898	The hydrogels were prepared by simple mixing of DG-XG or XGu with CNCs in water.
33332094	6	12	theme	liquid	1003:1008	arg1	mixture					1029:1035	a phase-separated mixture	1011:1035	a phase-separated mixture	1011:1035	Phase diagrams were established to identify the ratios of DG-XG or XGu to CNCs that yielded a viscous liquid, a phase-separated mixture, a simple gel, or a thermoresponsive gel.
33332094	7	13	theme	CNCs	1185:1188	arg1	coverage					1173:1180	the full surface coverage	1156:1180	the full surface coverage of CNCs	1156:1188	Gelation occurred at a DG-XG or XGu to CNC ratio higher than that needed for the full surface coverage of CNCs and required relatively high overall concentrations of both components (tested concentrations up to 20 g/L XG and 30 g/L CNCs).
33332094	7	14	theme	surface	1165:1171	arg1	coverage					1173:1180	the full surface coverage	1156:1180	the full surface coverage of CNCs	1156:1188	Gelation occurred at a DG-XG or XGu to CNC ratio higher than that needed for the full surface coverage of CNCs and required relatively high overall concentrations of both components (tested concentrations up to 20 g/L XG and 30 g/L CNCs).
33332094	1	15	theme	unique	108:113	arg1	properties					115:124	unique properties	108:124	unique properties	108:124	Thermoresponsive hydrogels present unique properties, such as tunable mechanical performance or changes in volume, which make them attractive for applications including wound healing dressings, drug delivery vehicles, and implants, among others.
33332094	9	16	from	layer	1523:1527	arg1	CNCs					1532:1535	CNCs	1532:1535	CNCs	1532:1535	Investigation of the adsorption behavior indicated that DG-XG formed a more rigid layer on CNCs compared to XGu.
33332094	6	17	theme	DG-XG	959:963	arg1	ratios					949:954	the ratios	945:954	the ratios of DG-XG or XGu to CNCs that yielded a viscous liquid, a phase-separated mixture, a simple gel, or a thermoresponsive gel	945:1076	Phase diagrams were established to identify the ratios of DG-XG or XGu to CNCs that yielded a viscous liquid, a phase-separated mixture, a simple gel, or a thermoresponsive gel.
33332094	7	18	theme	CNC	1118:1120	arg1	ratio					1122:1126	CNC ratio	1118:1126	CNC ratio higher than that needed for the full surface coverage of CNCs	1118:1188	Gelation occurred at a DG-XG or XGu to CNC ratio higher than that needed for the full surface coverage of CNCs and required relatively high overall concentrations of both components (tested concentrations up to 20 g/L XG and 30 g/L CNCs).
33332094	3	19	theme	thermoresponsive	499:514	arg1	XG					516:517	thermoresponsive XG	499:517	thermoresponsive XG	499:517	Starting from tamarind seed XG (XGt), thermoresponsive XG was obtained by enzymatic degalactosylation (DG-XG), which reduced the galactose residue content by ∼50% and imparted a reversible thermal transition.
33332094	7	20	dep	required	1194:1201	arg1	concentrations					1269:1282	tested concentrations	1262:1282	tested concentrations up to 20 g/L XG and 30 g/L CNCs	1262:1314	Gelation occurred at a DG-XG or XGu to CNC ratio higher than that needed for the full surface coverage of CNCs and required relatively high overall concentrations of both components (tested concentrations up to 20 g/L XG and 30 g/L CNCs).
33332094	2	21	theme	cellulose	430:438	arg1	CNCs					454:457	CNCs	454:457	CNCs	454:457	This work reports the implementation of bioinspired thermoresponsive hydrogels composed of xyloglucan (XG) and cellulose nanocrystals (CNCs).
33332094	2	21	theme	cellulose	430:438	arg1	nanocrystals					440:451	xyloglucan (XG) and cellulose nanocrystals	410:451	xyloglucan (XG) and cellulose nanocrystals (CNCs)	410:458	This work reports the implementation of bioinspired thermoresponsive hydrogels composed of xyloglucan (XG) and cellulose nanocrystals (CNCs).
33332094	8	22	theme	XG-CNC	1423:1428	arg1	complexes					1430:1438	XG-CNC complexes	1423:1438	XG-CNC complexes	1423:1438	This is likely a result of the increase in effective hydrodynamic volume of CNCs due to the formation of XG-CNC complexes.
33332094	6	23	theme	thermoresponsive	1057:1072	arg1	gel					1074:1076	a thermoresponsive gel	1055:1076	a thermoresponsive gel	1055:1076	Phase diagrams were established to identify the ratios of DG-XG or XGu to CNCs that yielded a viscous liquid, a phase-separated mixture, a simple gel, or a thermoresponsive gel.
33332094	1	24	from	changes	169:175	arg1	volume					180:185	volume	180:185	volume	180:185	Thermoresponsive hydrogels present unique properties, such as tunable mechanical performance or changes in volume, which make them attractive for applications including wound healing dressings, drug delivery vehicles, and implants, among others.
33332094	10	25	theme	thermal	1631:1637	arg1	transition					1639:1648	a reversible thermal transition	1618:1648	a reversible thermal transition	1618:1648	Rheological properties of the hydrogels were characterized, and a reversible thermal transition was found for DG-XG/CNC gels at 35 °C.
33332094	6	26	theme	simple	1040:1045	arg1	gel					1047:1049	a simple gel	1038:1049	a simple gel	1038:1049	Phase diagrams were established to identify the ratios of DG-XG or XGu to CNCs that yielded a viscous liquid, a phase-separated mixture, a simple gel, or a thermoresponsive gel.
33332094	3	27	theme	tamarind	475:482	arg1	XG					489:490	tamarind seed XG	475:490	tamarind seed XG (XGt)	475:496	Starting from tamarind seed XG (XGt), thermoresponsive XG was obtained by enzymatic degalactosylation (DG-XG), which reduced the galactose residue content by ∼50% and imparted a reversible thermal transition.
33332094	3	27	theme	tamarind	475:482	arg1	XGt					493:495	XGt	493:495	XGt	493:495	Starting from tamarind seed XG (XGt), thermoresponsive XG was obtained by enzymatic degalactosylation (DG-XG), which reduced the galactose residue content by ∼50% and imparted a reversible thermal transition.
33332094	0	28	theme	Thermoresponsive	12:27	arg1	Xyloglucan-Cellulose					29:48	Bioinspired Thermoresponsive Xyloglucan-Cellulose	0:48	Bioinspired Thermoresponsive Xyloglucan-Cellulose	0:48	Bioinspired Thermoresponsive Xyloglucan-Cellulose Nanocrystal Hydrogels.
33332094	7	29	theme	high	1214:1217	arg1	concentrations					1227:1240	relatively high overall concentrations	1203:1240	relatively high overall concentrations of both components	1203:1259	Gelation occurred at a DG-XG or XGu to CNC ratio higher than that needed for the full surface coverage of CNCs and required relatively high overall concentrations of both components (tested concentrations up to 20 g/L XG and 30 g/L CNCs).
33332094	8	30	from	increase	1349:1356	arg1	volume					1384:1389	effective hydrodynamic volume	1361:1389	effective hydrodynamic volume of CNCs due to the formation of XG-CNC complexes	1361:1438	This is likely a result of the increase in effective hydrodynamic volume of CNCs due to the formation of XG-CNC complexes.
33332094	6	31	dep	mixture	1029:1035	arg1	a					993:993	a	993:993	a	993:993	Phase diagrams were established to identify the ratios of DG-XG or XGu to CNCs that yielded a viscous liquid, a phase-separated mixture, a simple gel, or a thermoresponsive gel.
33332094	4	32	theme	native	678:683	arg1	composition					685:695	native composition	678:695	native composition	678:695	XG with native composition and comparable molar mass to DG-XG was produced by an ultrasonication treatment (XGu) for a direct comparison of behavior.
33332094	0	33	theme	Bioinspired	0:10	arg1	Xyloglucan-Cellulose					29:48	Bioinspired Thermoresponsive Xyloglucan-Cellulose	0:48	Bioinspired Thermoresponsive Xyloglucan-Cellulose	0:48	Bioinspired Thermoresponsive Xyloglucan-Cellulose Nanocrystal Hydrogels.
33332094	8	34	theme	hydrodynamic	1371:1382	arg1	volume					1384:1389	effective hydrodynamic volume	1361:1389	effective hydrodynamic volume of CNCs due to the formation of XG-CNC complexes	1361:1438	This is likely a result of the increase in effective hydrodynamic volume of CNCs due to the formation of XG-CNC complexes.
33332094	1	35	from	performance	154:164	arg1	volume					180:185	volume	180:185	volume	180:185	Thermoresponsive hydrogels present unique properties, such as tunable mechanical performance or changes in volume, which make them attractive for applications including wound healing dressings, drug delivery vehicles, and implants, among others.
33332094	9	36	theme	adsorption	1462:1471	arg1	behavior					1473:1480	the adsorption behavior	1458:1480	the adsorption behavior	1458:1480	Investigation of the adsorption behavior indicated that DG-XG formed a more rigid layer on CNCs compared to XGu.
33332094	5	37	theme	DG-XG	868:872	arg1	mixing					858:863	simple mixing	851:863	simple mixing of DG-XG or XGu with CNCs in water	851:898	The hydrogels were prepared by simple mixing of DG-XG or XGu with CNCs in water.
33332094	7	38	theme	g/L	1293:1295	arg1	XG					1297:1298	20 g/L XG	1290:1298	20 g/L XG	1290:1298	Gelation occurred at a DG-XG or XGu to CNC ratio higher than that needed for the full surface coverage of CNCs and required relatively high overall concentrations of both components (tested concentrations up to 20 g/L XG and 30 g/L CNCs).
33332094	8	39	theme	complexes	1430:1438	arg1	formation					1410:1418	the formation	1406:1418	the formation of XG-CNC complexes	1406:1438	This is likely a result of the increase in effective hydrodynamic volume of CNCs due to the formation of XG-CNC complexes.
33332094	0	40	dep	Hydrogels	62:70	arg1	Xyloglucan-Cellulose					29:48	Bioinspired Thermoresponsive Xyloglucan-Cellulose	0:48	Bioinspired Thermoresponsive Xyloglucan-Cellulose	0:48	Bioinspired Thermoresponsive Xyloglucan-Cellulose Nanocrystal Hydrogels.
33332094	1	41	theme	tunable	135:141	arg1	performance					154:164	tunable mechanical performance	135:164	tunable mechanical performance	135:164	Thermoresponsive hydrogels present unique properties, such as tunable mechanical performance or changes in volume, which make them attractive for applications including wound healing dressings, drug delivery vehicles, and implants, among others.
33332094	8	42	theme	CNCs	1394:1397	arg1	volume					1384:1389	effective hydrodynamic volume	1361:1389	effective hydrodynamic volume of CNCs due to the formation of XG-CNC complexes	1361:1438	This is likely a result of the increase in effective hydrodynamic volume of CNCs due to the formation of XG-CNC complexes.
33332094	11	43	theme	mechanical	1818:1827	arg1	properties					1829:1838	the mechanical properties	1814:1838	the mechanical properties	1814:1838	This thermoresponsive behavior provides opportunities to apply this system widely, especially in the biomedical field, where the mechanical properties could be further tuned by adjusting the CNC content.
33332094	3	44	theme	thermal	650:656	arg1	transition					658:667	a reversible thermal transition	637:667	a reversible thermal transition	637:667	Starting from tamarind seed XG (XGt), thermoresponsive XG was obtained by enzymatic degalactosylation (DG-XG), which reduced the galactose residue content by ∼50% and imparted a reversible thermal transition.
33332094	1	45	theme	mechanical	143:152	arg1	performance					154:164	tunable mechanical performance	135:164	tunable mechanical performance	135:164	Thermoresponsive hydrogels present unique properties, such as tunable mechanical performance or changes in volume, which make them attractive for applications including wound healing dressings, drug delivery vehicles, and implants, among others.
33332094	1	46	theme	wound	242:246	arg1	healing					248:254	wound healing	242:254	wound healing dressings	242:264	Thermoresponsive hydrogels present unique properties, such as tunable mechanical performance or changes in volume, which make them attractive for applications including wound healing dressings, drug delivery vehicles, and implants, among others.
33332094	11	47	theme	biomedical	1790:1799	arg1	field					1801:1805	the biomedical field	1786:1805	the biomedical field	1786:1805	This thermoresponsive behavior provides opportunities to apply this system widely, especially in the biomedical field, where the mechanical properties could be further tuned by adjusting the CNC content.
33332094	10	48	theme	DG-XG/CNC	1664:1672	arg1	gels					1674:1677	DG-XG/CNC gels	1664:1677	DG-XG/CNC gels	1664:1677	Rheological properties of the hydrogels were characterized, and a reversible thermal transition was found for DG-XG/CNC gels at 35 °C.
33332094	9	49	theme	behavior	1473:1480	arg1	Investigation					1441:1453	Investigation	1441:1453	Investigation of the adsorption behavior	1441:1480	Investigation of the adsorption behavior indicated that DG-XG formed a more rigid layer on CNCs compared to XGu.
33332094	1	50	theme	healing	248:254	arg1	dressings					256:264	wound healing dressings	242:264	wound healing dressings	242:264	Thermoresponsive hydrogels present unique properties, such as tunable mechanical performance or changes in volume, which make them attractive for applications including wound healing dressings, drug delivery vehicles, and implants, among others.
33332094	4	51	theme	behavior	810:817	arg1	comparison					796:805	a direct comparison	787:805	a direct comparison of behavior	787:817	XG with native composition and comparable molar mass to DG-XG was produced by an ultrasonication treatment (XGu) for a direct comparison of behavior.
33332094	6	52	theme	phase-separated	1013:1027	arg1	mixture					1029:1035	a phase-separated mixture	1011:1035	a phase-separated mixture	1011:1035	Phase diagrams were established to identify the ratios of DG-XG or XGu to CNCs that yielded a viscous liquid, a phase-separated mixture, a simple gel, or a thermoresponsive gel.
33332094	3	53	theme	seed	484:487	arg1	XG					489:490	tamarind seed XG	475:490	tamarind seed XG (XGt)	475:496	Starting from tamarind seed XG (XGt), thermoresponsive XG was obtained by enzymatic degalactosylation (DG-XG), which reduced the galactose residue content by ∼50% and imparted a reversible thermal transition.
33332094	3	53	theme	seed	484:487	arg1	XGt					493:495	XGt	493:495	XGt	493:495	Starting from tamarind seed XG (XGt), thermoresponsive XG was obtained by enzymatic degalactosylation (DG-XG), which reduced the galactose residue content by ∼50% and imparted a reversible thermal transition.
33332094	5	54	theme	simple	851:856	arg1	mixing					858:863	simple mixing	851:863	simple mixing of DG-XG or XGu with CNCs in water	851:898	The hydrogels were prepared by simple mixing of DG-XG or XGu with CNCs in water.
33332094	7	55	theme	g/L	1307:1309	arg1	CNCs					1311:1314	30 g/L CNCs	1304:1314	30 g/L CNCs	1304:1314	Gelation occurred at a DG-XG or XGu to CNC ratio higher than that needed for the full surface coverage of CNCs and required relatively high overall concentrations of both components (tested concentrations up to 20 g/L XG and 30 g/L CNCs).
33332094	4	56	with	XG	670:671	arg1	composition					685:695	native composition	678:695	native composition	678:695	XG with native composition and comparable molar mass to DG-XG was produced by an ultrasonication treatment (XGu) for a direct comparison of behavior.
33332094	4	56	with	XG	670:671	arg1	mass					718:721	comparable molar mass	701:721	comparable molar mass	701:721	XG with native composition and comparable molar mass to DG-XG was produced by an ultrasonication treatment (XGu) for a direct comparison of behavior.
33332094	6	57	theme	viscous	995:1001	arg1	mixture					1029:1035	a phase-separated mixture	1011:1035	a phase-separated mixture	1011:1035	Phase diagrams were established to identify the ratios of DG-XG or XGu to CNCs that yielded a viscous liquid, a phase-separated mixture, a simple gel, or a thermoresponsive gel.
33332094	8	58	from	volume	1384:1389	arg1	result					1335:1340	a result	1333:1340	a result of the increase in effective hydrodynamic volume of CNCs due to the formation of XG-CNC complexes	1333:1438	This is likely a result of the increase in effective hydrodynamic volume of CNCs due to the formation of XG-CNC complexes.
33332094	8	58	from	volume	1384:1389	arg1	This					1318:1321	This	1318:1321	This	1318:1321	This is likely a result of the increase in effective hydrodynamic volume of CNCs due to the formation of XG-CNC complexes.
33332094	3	59	theme	reversible	639:648	arg1	transition					658:667	a reversible thermal transition	637:667	a reversible thermal transition	637:667	Starting from tamarind seed XG (XGt), thermoresponsive XG was obtained by enzymatic degalactosylation (DG-XG), which reduced the galactose residue content by ∼50% and imparted a reversible thermal transition.
33332094	3	60	theme	galactose	590:598	arg1	residue					600:606	the galactose residue	586:606	the galactose residue content	586:614	Starting from tamarind seed XG (XGt), thermoresponsive XG was obtained by enzymatic degalactosylation (DG-XG), which reduced the galactose residue content by ∼50% and imparted a reversible thermal transition.
33332094	2	61	theme	hydrogels	388:396	arg1	implementation					341:354	the implementation	337:354	the implementation of bioinspired thermoresponsive hydrogels composed of xyloglucan (XG) and cellulose nanocrystals (CNCs)	337:458	This work reports the implementation of bioinspired thermoresponsive hydrogels composed of xyloglucan (XG) and cellulose nanocrystals (CNCs).
33332094	11	62	theme	thermoresponsive	1694:1709	arg1	behavior					1711:1718	This thermoresponsive behavior	1689:1718	This thermoresponsive behavior	1689:1718	This thermoresponsive behavior provides opportunities to apply this system widely, especially in the biomedical field, where the mechanical properties could be further tuned by adjusting the CNC content.
33332094	7	63	theme	components	1250:1259	arg1	concentrations					1227:1240	relatively high overall concentrations	1203:1240	relatively high overall concentrations of both components	1203:1259	Gelation occurred at a DG-XG or XGu to CNC ratio higher than that needed for the full surface coverage of CNCs and required relatively high overall concentrations of both components (tested concentrations up to 20 g/L XG and 30 g/L CNCs).
33332094	8	64	theme	effective	1361:1369	arg1	volume					1384:1389	effective hydrodynamic volume	1361:1389	effective hydrodynamic volume of CNCs due to the formation of XG-CNC complexes	1361:1438	This is likely a result of the increase in effective hydrodynamic volume of CNCs due to the formation of XG-CNC complexes.
33332094	3	65	theme	residue	600:606	arg1	content					608:614	the galactose residue content	586:614	the galactose residue content	586:614	Starting from tamarind seed XG (XGt), thermoresponsive XG was obtained by enzymatic degalactosylation (DG-XG), which reduced the galactose residue content by ∼50% and imparted a reversible thermal transition.
33332094	2	66	theme	thermoresponsive	371:386	arg1	hydrogels					388:396	bioinspired thermoresponsive hydrogels	359:396	bioinspired thermoresponsive hydrogels composed of xyloglucan (XG) and cellulose nanocrystals (CNCs)	359:458	This work reports the implementation of bioinspired thermoresponsive hydrogels composed of xyloglucan (XG) and cellulose nanocrystals (CNCs).
33332094	2	67	theme	bioinspired	359:369	arg1	hydrogels					388:396	bioinspired thermoresponsive hydrogels	359:396	bioinspired thermoresponsive hydrogels composed of xyloglucan (XG) and cellulose nanocrystals (CNCs)	359:458	This work reports the implementation of bioinspired thermoresponsive hydrogels composed of xyloglucan (XG) and cellulose nanocrystals (CNCs).
33332094	4	68	theme	ultrasonication	751:765	arg1	XGu					778:780	XGu	778:780	XGu	778:780	XG with native composition and comparable molar mass to DG-XG was produced by an ultrasonication treatment (XGu) for a direct comparison of behavior.
33332094	4	68	theme	ultrasonication	751:765	arg1	treatment					767:775	an ultrasonication treatment	748:775	an ultrasonication treatment (XGu) for a direct comparison of behavior	748:817	XG with native composition and comparable molar mass to DG-XG was produced by an ultrasonication treatment (XGu) for a direct comparison of behavior.
33332094	5	69	with	mixing	858:863	arg1	CNCs					886:889	CNCs	886:889	CNCs in water	886:898	The hydrogels were prepared by simple mixing of DG-XG or XGu with CNCs in water.
33332094	9	70	theme	rigid	1517:1521	arg1	layer					1523:1527	a more rigid layer	1510:1527	a more rigid layer on CNCs	1510:1535	Investigation of the adsorption behavior indicated that DG-XG formed a more rigid layer on CNCs compared to XGu.
33332094	3	71	theme	enzymatic	535:543	arg1	DG-XG					564:568	DG-XG	564:568	DG-XG	564:568	Starting from tamarind seed XG (XGt), thermoresponsive XG was obtained by enzymatic degalactosylation (DG-XG), which reduced the galactose residue content by ∼50% and imparted a reversible thermal transition.
33332094	3	71	theme	enzymatic	535:543	arg1	degalactosylation					545:561	enzymatic degalactosylation	535:561	enzymatic degalactosylation (DG-XG)	535:569	Starting from tamarind seed XG (XGt), thermoresponsive XG was obtained by enzymatic degalactosylation (DG-XG), which reduced the galactose residue content by ∼50% and imparted a reversible thermal transition.
33332094	1	72	theme	drug	267:270	arg1	vehicles					281:288	drug delivery vehicles	267:288	drug delivery vehicles	267:288	Thermoresponsive hydrogels present unique properties, such as tunable mechanical performance or changes in volume, which make them attractive for applications including wound healing dressings, drug delivery vehicles, and implants, among others.
33332094	8	73	theme	increase	1349:1356	arg1	result					1335:1340	a result	1333:1340	a result of the increase in effective hydrodynamic volume of CNCs due to the formation of XG-CNC complexes	1333:1438	This is likely a result of the increase in effective hydrodynamic volume of CNCs due to the formation of XG-CNC complexes.
33332094	8	73	theme	increase	1349:1356	arg1	This					1318:1321	This	1318:1321	This	1318:1321	This is likely a result of the increase in effective hydrodynamic volume of CNCs due to the formation of XG-CNC complexes.
33332094	7	74	theme	overall	1219:1225	arg1	concentrations					1227:1240	relatively high overall concentrations	1203:1240	relatively high overall concentrations of both components	1203:1259	Gelation occurred at a DG-XG or XGu to CNC ratio higher than that needed for the full surface coverage of CNCs and required relatively high overall concentrations of both components (tested concentrations up to 20 g/L XG and 30 g/L CNCs).
33332094	1	75	theme	Thermoresponsive	73:88	arg1	hydrogels					90:98	Thermoresponsive hydrogels	73:98	Thermoresponsive hydrogels	73:98	Thermoresponsive hydrogels present unique properties, such as tunable mechanical performance or changes in volume, which make them attractive for applications including wound healing dressings, drug delivery vehicles, and implants, among others.
33332094	11	76	theme	CNC	1880:1882	arg1	content					1884:1890	the CNC content	1876:1890	the CNC content	1876:1890	This thermoresponsive behavior provides opportunities to apply this system widely, especially in the biomedical field, where the mechanical properties could be further tuned by adjusting the CNC content.
33332094	10	77	theme	reversible	1620:1629	arg1	transition					1639:1648	a reversible thermal transition	1618:1648	a reversible thermal transition	1618:1648	Rheological properties of the hydrogels were characterized, and a reversible thermal transition was found for DG-XG/CNC gels at 35 °C.
33332094	7	78	theme	tested	1262:1267	arg1	concentrations					1269:1282	tested concentrations	1262:1282	tested concentrations up to 20 g/L XG and 30 g/L CNCs	1262:1314	Gelation occurred at a DG-XG or XGu to CNC ratio higher than that needed for the full surface coverage of CNCs and required relatively high overall concentrations of both components (tested concentrations up to 20 g/L XG and 30 g/L CNCs).
33332094	1	79	theme	delivery	272:279	arg1	vehicles					281:288	drug delivery vehicles	267:288	drug delivery vehicles	267:288	Thermoresponsive hydrogels present unique properties, such as tunable mechanical performance or changes in volume, which make them attractive for applications including wound healing dressings, drug delivery vehicles, and implants, among others.
33332094	6	80	theme	Phase	901:905	arg1	diagrams					907:914	Phase diagrams	901:914	Phase diagrams	901:914	Phase diagrams were established to identify the ratios of DG-XG or XGu to CNCs that yielded a viscous liquid, a phase-separated mixture, a simple gel, or a thermoresponsive gel.
33332094	4	81	theme	direct	789:794	arg1	comparison					796:805	a direct comparison	787:805	a direct comparison of behavior	787:817	XG with native composition and comparable molar mass to DG-XG was produced by an ultrasonication treatment (XGu) for a direct comparison of behavior.
33332094	2	82	theme	xyloglucan	410:419	arg1	CNCs					454:457	CNCs	454:457	CNCs	454:457	This work reports the implementation of bioinspired thermoresponsive hydrogels composed of xyloglucan (XG) and cellulose nanocrystals (CNCs).
33332094	2	82	theme	xyloglucan	410:419	arg1	nanocrystals					440:451	xyloglucan (XG) and cellulose nanocrystals	410:451	xyloglucan (XG) and cellulose nanocrystals (CNCs)	410:458	This work reports the implementation of bioinspired thermoresponsive hydrogels composed of xyloglucan (XG) and cellulose nanocrystals (CNCs).
33332094	7	83	theme	full	1160:1163	arg1	coverage					1173:1180	the full surface coverage	1156:1180	the full surface coverage of CNCs	1156:1188	Gelation occurred at a DG-XG or XGu to CNC ratio higher than that needed for the full surface coverage of CNCs and required relatively high overall concentrations of both components (tested concentrations up to 20 g/L XG and 30 g/L CNCs).
33332094	10	84	located	found	1654:1658	arg1	°C					1685:1686	35 °C	1682:1686	35 °C	1682:1686	Rheological properties of the hydrogels were characterized, and a reversible thermal transition was found for DG-XG/CNC gels at 35 °C.
33332094	10	84	located	found	1654:1658	arg2	transition					1639:1648	a reversible thermal transition	1618:1648	a reversible thermal transition	1618:1648	Rheological properties of the hydrogels were characterized, and a reversible thermal transition was found for DG-XG/CNC gels at 35 °C.
33812591	1	0	dep	Three-dimensional	87:103	arg1	3D					106:107	3D	106:107	3D	106:107	Three-dimensional (3D) printing is a promising method to prepare scaffolds for tissue regeneration.
33812591	7	1	theme	collagen/chitosan	1403:1419	arg1	bioinks					1421:1427	the hybrid collagen/chitosan bioinks	1392:1427	the hybrid collagen/chitosan bioinks	1392:1427	Therefore, chitosan can improve the printability of collagen and the hybrid collagen/chitosan bioinks can be printed into scaffolds with regulated properties, thus can fit different applications in tissue engineering.
33812591	6	2	theme	scaffolds	1281:1289	arg1	bottom					1267:1272	the bottom	1263:1272	the bottom of the scaffolds	1263:1289	Cell viability assay presented vigorous cell growth on the surface of scaffolds, meanwhile, live cells were also found inside and at the bottom of the scaffolds, indicating the migration of cells.
33812591	5	3	theme	%	983:983	arg1	collagen					991:998	2% (m/v) collagen	982:998	2% (m/v) collagen	982:998	Moreover, the scaffolds containing 2% (m/v) collagen and 2% (m/v) chitosan had a homogeneous and compact honeycomb-like structure, demonstrating the strengthening effect of chitosan.
33812591	4	4	theme	Rheological	546:556	arg1	tests					558:562	Rheological tests	546:562	Rheological tests	546:562	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	4	5	theme	chitosan	926:933	arg1	proportion					935:944	chitosan proportion	926:944	chitosan proportion	926:944	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	4	6	theme	Young	874:878	arg1	modulus					882:888	Young's modulus	874:888	Young's modulus	874:888	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	4	7	theme	degradation	804:814	arg1	rate					816:819	their degradation rate	798:819	their degradation rate	798:819	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	4	8	from	Chitosan	723:730	arg1	behavior					618:625	sound shear thinning behavior	597:625	sound shear thinning behavior	597:625	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	4	8	from	Chitosan	723:730	arg1	temperature					694:704	the gelation temperature	681:704	the gelation temperature from 7 to 10 °C. Chitosan	681:730	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	4	8	from	Chitosan	723:730	arg1	variation					649:657	dramatical viscosity variation	628:657	dramatical viscosity variation with temperature	628:674	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	4	9	theme	viscosity	639:647	arg1	variation					649:657	dramatical viscosity variation	628:657	dramatical viscosity variation with temperature	628:674	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	3	10	theme	tunable	356:362	arg1	bioinks					389:395	tunable hybrid collagen/chitosan bioinks	356:395	tunable hybrid collagen/chitosan bioinks	356:395	Here, we prepared a series of tunable hybrid collagen/chitosan bioinks with significantly improved printability through hydrogen bond interaction and printed them into scaffolds by carefully controlling the temperature.
33812591	7	11	theme	regulated	1464:1472	arg1	properties					1474:1483	regulated properties	1464:1483	regulated properties	1464:1483	Therefore, chitosan can improve the printability of collagen and the hybrid collagen/chitosan bioinks can be printed into scaffolds with regulated properties, thus can fit different applications in tissue engineering.
33812591	6	12	theme	scaffolds	1200:1208	arg1	surface					1189:1195	the surface	1185:1195	the surface of scaffolds, meanwhile, live cells were also found inside and at the bottom of the scaffolds, indicating the migration of cells	1185:1324	Cell viability assay presented vigorous cell growth on the surface of scaffolds, meanwhile, live cells were also found inside and at the bottom of the scaffolds, indicating the migration of cells.
33812591	6	13	attach	presented	1151:1159	arg2	assay					1145:1149	Cell viability assay	1130:1149	Cell viability assay	1130:1149	Cell viability assay presented vigorous cell growth on the surface of scaffolds, meanwhile, live cells were also found inside and at the bottom of the scaffolds, indicating the migration of cells.
33812591	6	13	attach	presented	1151:1159	arg1	surface					1189:1195	the surface	1185:1195	the surface of scaffolds, meanwhile, live cells were also found inside and at the bottom of the scaffolds, indicating the migration of cells	1185:1324	Cell viability assay presented vigorous cell growth on the surface of scaffolds, meanwhile, live cells were also found inside and at the bottom of the scaffolds, indicating the migration of cells.
33812591	5	14	dep	%	983:983	arg1	m/v					986:988	m/v	986:988	m/v	986:988	Moreover, the scaffolds containing 2% (m/v) collagen and 2% (m/v) chitosan had a homogeneous and compact honeycomb-like structure, demonstrating the strengthening effect of chitosan.
33812591	4	15	theme	dramatical	628:637	arg1	variation					649:657	dramatical viscosity variation	628:657	dramatical viscosity variation with temperature	628:674	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	5	16	theme	chitosan	1120:1127	arg1	effect					1110:1115	the strengthening effect	1092:1115	the strengthening effect of chitosan	1092:1127	Moreover, the scaffolds containing 2% (m/v) collagen and 2% (m/v) chitosan had a homogeneous and compact honeycomb-like structure, demonstrating the strengthening effect of chitosan.
33812591	4	17	theme	scaffolds	781:789	arg1	ratio					760:764	the swelling ratio	747:764	the swelling ratio of the printed scaffolds	747:789	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	5	18	theme	2	1004:1004	arg1	%					1005:1005	%	1005:1005	%	1005:1005	Moreover, the scaffolds containing 2% (m/v) collagen and 2% (m/v) chitosan had a homogeneous and compact honeycomb-like structure, demonstrating the strengthening effect of chitosan.
33812591	2	19	dep	engineering	254:264	arg1	scaffold					266:273	scaffold	266:273	scaffold	266:273	Collagen and chitosan composites are superior materials for tissue engineering scaffold but rarely printed due to their poor printability.
33812591	2	19	dep	engineering	254:264	arg1	printed					286:292	printed	286:292	printed	286:292	Collagen and chitosan composites are superior materials for tissue engineering scaffold but rarely printed due to their poor printability.
33812591	0	20	theme	chitosan	44:51	arg1	printing					19:26	Low-temperature 3D printing	0:26	Low-temperature 3D printing of collagen and chitosan	0:51	Low-temperature 3D printing of collagen and chitosan composite for tissue engineering.
33812591	4	21	with	behavior	618:625	arg1	temperature					664:674	temperature	664:674	temperature	664:674	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	1	22	theme	tissue	166:171	arg1	regeneration					173:184	tissue regeneration	166:184	tissue regeneration	166:184	Three-dimensional (3D) printing is a promising method to prepare scaffolds for tissue regeneration.
33812591	0	23	theme	3D	16:17	arg1	printing					19:26	Low-temperature 3D printing	0:26	Low-temperature 3D printing of collagen and chitosan	0:51	Low-temperature 3D printing of collagen and chitosan composite for tissue engineering.
33812591	4	24	theme	tensile	894:900	arg1	strength					902:909	tensile strength	894:909	tensile strength	894:909	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	1	25	theme	Three-dimensional	87:103	arg1	printing					110:117	Three-dimensional (3D) printing	87:117	Three-dimensional (3D) printing	87:117	Three-dimensional (3D) printing is a promising method to prepare scaffolds for tissue regeneration.
33812591	1	25	theme	Three-dimensional	87:103	arg1	method					134:139	a promising method	122:139	a promising method to prepare scaffolds for tissue regeneration	122:184	Three-dimensional (3D) printing is a promising method to prepare scaffolds for tissue regeneration.
33812591	3	26	theme	hydrogen	446:453	arg1	interaction					460:470	hydrogen bond interaction	446:470	hydrogen bond interaction	446:470	Here, we prepared a series of tunable hybrid collagen/chitosan bioinks with significantly improved printability through hydrogen bond interaction and printed them into scaffolds by carefully controlling the temperature.
33812591	0	27	theme	Low-temperature	0:14	arg1	printing					19:26	Low-temperature 3D printing	0:26	Low-temperature 3D printing of collagen and chitosan	0:51	Low-temperature 3D printing of collagen and chitosan composite for tissue engineering.
33812591	4	28	theme	sound	597:601	arg1	behavior					618:625	sound shear thinning behavior	597:625	sound shear thinning behavior	597:625	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	4	29	theme	printed	773:779	arg1	scaffolds					781:789	the printed scaffolds	769:789	the printed scaffolds	769:789	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	3	30	theme	bond	455:458	arg1	interaction					460:470	hydrogen bond interaction	446:470	hydrogen bond interaction	446:470	Here, we prepared a series of tunable hybrid collagen/chitosan bioinks with significantly improved printability through hydrogen bond interaction and printed them into scaffolds by carefully controlling the temperature.
33812591	3	31	theme	hybrid	364:369	arg1	bioinks					389:395	tunable hybrid collagen/chitosan bioinks	356:395	tunable hybrid collagen/chitosan bioinks	356:395	Here, we prepared a series of tunable hybrid collagen/chitosan bioinks with significantly improved printability through hydrogen bond interaction and printed them into scaffolds by carefully controlling the temperature.
33812591	4	32	dep	10 °C.	716:721	arg1	to					713:714	to	713:714	to	713:714	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	6	33	dep	scaffolds	1200:1208	arg1	found					1243:1247	found	1243:1247	found inside and at the bottom of the scaffolds	1243:1289	Cell viability assay presented vigorous cell growth on the surface of scaffolds, meanwhile, live cells were also found inside and at the bottom of the scaffolds, indicating the migration of cells.
33812591	5	34	theme	2	982:982	arg1	%					983:983	%	983:983	%	983:983	Moreover, the scaffolds containing 2% (m/v) collagen and 2% (m/v) chitosan had a homogeneous and compact honeycomb-like structure, demonstrating the strengthening effect of chitosan.
33812591	2	35	theme	poor	307:310	arg1	printability					312:323	their poor printability	301:323	their poor printability	301:323	Collagen and chitosan composites are superior materials for tissue engineering scaffold but rarely printed due to their poor printability.
33812591	7	36	theme	collagen	1379:1386	arg1	printability					1363:1374	the printability	1359:1374	the printability of collagen	1359:1386	Therefore, chitosan can improve the printability of collagen and the hybrid collagen/chitosan bioinks can be printed into scaffolds with regulated properties, thus can fit different applications in tissue engineering.
33812591	3	37	theme	collagen/chitosan	371:387	arg1	bioinks					389:395	tunable hybrid collagen/chitosan bioinks	356:395	tunable hybrid collagen/chitosan bioinks	356:395	Here, we prepared a series of tunable hybrid collagen/chitosan bioinks with significantly improved printability through hydrogen bond interaction and printed them into scaffolds by carefully controlling the temperature.
33812591	4	38	theme	thinning	609:616	arg1	behavior					618:625	sound shear thinning behavior	597:625	sound shear thinning behavior	597:625	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	6	39	theme	cell	1170:1173	arg1	growth					1175:1180	vigorous cell growth	1161:1180	vigorous cell growth	1161:1180	Cell viability assay presented vigorous cell growth on the surface of scaffolds, meanwhile, live cells were also found inside and at the bottom of the scaffolds, indicating the migration of cells.
33812591	5	40	theme	homogeneous	1028:1038	arg1	structure					1067:1075	a homogeneous and compact honeycomb-like structure	1026:1075	a homogeneous and compact honeycomb-like structure	1026:1075	Moreover, the scaffolds containing 2% (m/v) collagen and 2% (m/v) chitosan had a homogeneous and compact honeycomb-like structure, demonstrating the strengthening effect of chitosan.
33812591	4	41	theme	shear	603:607	arg1	behavior					618:625	sound shear thinning behavior	597:625	sound shear thinning behavior	597:625	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	5	42	theme	compact	1044:1050	arg1	structure					1067:1075	a homogeneous and compact honeycomb-like structure	1026:1075	a homogeneous and compact honeycomb-like structure	1026:1075	Moreover, the scaffolds containing 2% (m/v) collagen and 2% (m/v) chitosan had a homogeneous and compact honeycomb-like structure, demonstrating the strengthening effect of chitosan.
33812591	6	43	theme	meanwhile	1211:1219	arg1	cells					1227:1231	meanwhile, live cells	1211:1231	meanwhile, live cells	1211:1231	Cell viability assay presented vigorous cell growth on the surface of scaffolds, meanwhile, live cells were also found inside and at the bottom of the scaffolds, indicating the migration of cells.
33812591	0	44	theme	collagen	31:38	arg1	printing					19:26	Low-temperature 3D printing	0:26	Low-temperature 3D printing of collagen and chitosan	0:51	Low-temperature 3D printing of collagen and chitosan composite for tissue engineering.
33812591	6	45	theme	cells	1320:1324	arg1	migration					1307:1315	the migration	1303:1315	the migration of cells	1303:1324	Cell viability assay presented vigorous cell growth on the surface of scaffolds, meanwhile, live cells were also found inside and at the bottom of the scaffolds, indicating the migration of cells.
33812591	5	46	theme	strengthening	1096:1108	arg1	effect					1110:1115	the strengthening effect	1092:1115	the strengthening effect of chitosan	1092:1127	Moreover, the scaffolds containing 2% (m/v) collagen and 2% (m/v) chitosan had a homogeneous and compact honeycomb-like structure, demonstrating the strengthening effect of chitosan.
33812591	3	47	with	series	346:351	arg1	printability					425:436	significantly improved printability	402:436	significantly improved printability	402:436	Here, we prepared a series of tunable hybrid collagen/chitosan bioinks with significantly improved printability through hydrogen bond interaction and printed them into scaffolds by carefully controlling the temperature.
33812591	4	48	theme	gelation	685:692	arg1	temperature					694:704	the gelation temperature	681:704	the gelation temperature from 7 to 10 °C. Chitosan	681:730	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	5	49	theme	honeycomb-like	1052:1065	arg1	structure					1067:1075	a homogeneous and compact honeycomb-like structure	1026:1075	a homogeneous and compact honeycomb-like structure	1026:1075	Moreover, the scaffolds containing 2% (m/v) collagen and 2% (m/v) chitosan had a homogeneous and compact honeycomb-like structure, demonstrating the strengthening effect of chitosan.
33812591	2	50	theme	superior	224:231	arg1	materials					233:241	superior materials	224:241	superior materials for tissue engineering scaffold but rarely printed due to their poor printability	224:323	Collagen and chitosan composites are superior materials for tissue engineering scaffold but rarely printed due to their poor printability.
33812591	2	50	theme	superior	224:231	arg1	composites					209:218	Collagen and chitosan composites	187:218	Collagen and chitosan composites	187:218	Collagen and chitosan composites are superior materials for tissue engineering scaffold but rarely printed due to their poor printability.
33812591	5	51	contain	containing	971:980	arg1	scaffolds					961:969	the scaffolds	957:969	the scaffolds containing 2% (m/v) collagen and 2% (m/v) chitosan	957:1020	Moreover, the scaffolds containing 2% (m/v) collagen and 2% (m/v) chitosan had a homogeneous and compact honeycomb-like structure, demonstrating the strengthening effect of chitosan.
33812591	5	51	contain	containing	971:980	arg2	chitosan					1013:1020	2% (m/v) chitosan	1004:1020	2% (m/v) chitosan	1004:1020	Moreover, the scaffolds containing 2% (m/v) collagen and 2% (m/v) chitosan had a homogeneous and compact honeycomb-like structure, demonstrating the strengthening effect of chitosan.
33812591	5	51	contain	containing	971:980	arg2	collagen					991:998	2% (m/v) collagen	982:998	2% (m/v) collagen	982:998	Moreover, the scaffolds containing 2% (m/v) collagen and 2% (m/v) chitosan had a homogeneous and compact honeycomb-like structure, demonstrating the strengthening effect of chitosan.
33812591	6	52	theme	vigorous	1161:1168	arg1	growth					1175:1180	vigorous cell growth	1161:1180	vigorous cell growth	1161:1180	Cell viability assay presented vigorous cell growth on the surface of scaffolds, meanwhile, live cells were also found inside and at the bottom of the scaffolds, indicating the migration of cells.
33812591	3	53	theme	improved	416:423	arg1	printability					425:436	significantly improved printability	402:436	significantly improved printability	402:436	Here, we prepared a series of tunable hybrid collagen/chitosan bioinks with significantly improved printability through hydrogen bond interaction and printed them into scaffolds by carefully controlling the temperature.
33812591	4	54	with	temperature	694:704	arg1	temperature					664:674	temperature	664:674	temperature	664:674	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	4	55	with	variation	649:657	arg1	temperature					664:674	temperature	664:674	temperature	664:674	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	4	56	theme	swelling	751:758	arg1	ratio					760:764	the swelling ratio	747:764	the swelling ratio of the printed scaffolds	747:789	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	7	57	with	scaffolds	1449:1457	arg1	properties					1474:1483	regulated properties	1464:1483	regulated properties	1464:1483	Therefore, chitosan can improve the printability of collagen and the hybrid collagen/chitosan bioinks can be printed into scaffolds with regulated properties, thus can fit different applications in tissue engineering.
33812591	4	58	theme	printable	575:583	arg1	bioinks					585:591	the printable bioinks	571:591	the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan	571:730	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	2	59	theme	chitosan	200:207	arg1	materials					233:241	superior materials	224:241	superior materials for tissue engineering scaffold but rarely printed due to their poor printability	224:323	Collagen and chitosan composites are superior materials for tissue engineering scaffold but rarely printed due to their poor printability.
33812591	2	59	theme	chitosan	200:207	arg1	composites					209:218	Collagen and chitosan composites	187:218	Collagen and chitosan composites	187:218	Collagen and chitosan composites are superior materials for tissue engineering scaffold but rarely printed due to their poor printability.
33812591	4	60	theme	collagen	836:843	arg1	proportion					845:854	collagen proportion	836:854	collagen proportion	836:854	Rheological tests proved the printable bioinks had sound shear thinning behavior, dramatical viscosity variation with temperature, and the gelation temperature from 7 to 10 °C. Chitosan could decrease the swelling ratio of the printed scaffolds, while their degradation rate increased with collagen proportion and the values of Young's modulus and tensile strength increased with chitosan proportion.
33812591	6	61	theme	viability	1135:1143	arg1	assay					1145:1149	Cell viability assay	1130:1149	Cell viability assay	1130:1149	Cell viability assay presented vigorous cell growth on the surface of scaffolds, meanwhile, live cells were also found inside and at the bottom of the scaffolds, indicating the migration of cells.
33812591	7	62	theme	hybrid	1396:1401	arg1	bioinks					1421:1427	the hybrid collagen/chitosan bioinks	1392:1427	the hybrid collagen/chitosan bioinks	1392:1427	Therefore, chitosan can improve the printability of collagen and the hybrid collagen/chitosan bioinks can be printed into scaffolds with regulated properties, thus can fit different applications in tissue engineering.
33812591	5	63	theme	%	1005:1005	arg1	chitosan					1013:1020	2% (m/v) chitosan	1004:1020	2% (m/v) chitosan	1004:1020	Moreover, the scaffolds containing 2% (m/v) collagen and 2% (m/v) chitosan had a homogeneous and compact honeycomb-like structure, demonstrating the strengthening effect of chitosan.
33812591	7	64	theme	different	1499:1507	arg1	applications					1509:1520	different applications	1499:1520	different applications in tissue engineering	1499:1542	Therefore, chitosan can improve the printability of collagen and the hybrid collagen/chitosan bioinks can be printed into scaffolds with regulated properties, thus can fit different applications in tissue engineering.
33812591	1	65	theme	promising	124:132	arg1	printing					110:117	Three-dimensional (3D) printing	87:117	Three-dimensional (3D) printing	87:117	Three-dimensional (3D) printing is a promising method to prepare scaffolds for tissue regeneration.
33812591	1	65	theme	promising	124:132	arg1	method					134:139	a promising method	122:139	a promising method to prepare scaffolds for tissue regeneration	122:184	Three-dimensional (3D) printing is a promising method to prepare scaffolds for tissue regeneration.
33812591	0	66	theme	tissue	67:72	arg1	engineering					74:84	tissue engineering	67:84	tissue engineering	67:84	Low-temperature 3D printing of collagen and chitosan composite for tissue engineering.
33812591	2	67	theme	Collagen	187:194	arg1	materials					233:241	superior materials	224:241	superior materials for tissue engineering scaffold but rarely printed due to their poor printability	224:323	Collagen and chitosan composites are superior materials for tissue engineering scaffold but rarely printed due to their poor printability.
33812591	2	67	theme	Collagen	187:194	arg1	composites					209:218	Collagen and chitosan composites	187:218	Collagen and chitosan composites	187:218	Collagen and chitosan composites are superior materials for tissue engineering scaffold but rarely printed due to their poor printability.
33812591	5	68	dep	%	1005:1005	arg1	m/v					1008:1010	m/v	1008:1010	m/v	1008:1010	Moreover, the scaffolds containing 2% (m/v) collagen and 2% (m/v) chitosan had a homogeneous and compact honeycomb-like structure, demonstrating the strengthening effect of chitosan.
33812591	6	69	theme	Cell	1130:1133	arg1	assay					1145:1149	Cell viability assay	1130:1149	Cell viability assay	1130:1149	Cell viability assay presented vigorous cell growth on the surface of scaffolds, meanwhile, live cells were also found inside and at the bottom of the scaffolds, indicating the migration of cells.
33812591	5	70	contain	had	1022:1024	arg1	scaffolds					961:969	the scaffolds	957:969	the scaffolds containing 2% (m/v) collagen and 2% (m/v) chitosan	957:1020	Moreover, the scaffolds containing 2% (m/v) collagen and 2% (m/v) chitosan had a homogeneous and compact honeycomb-like structure, demonstrating the strengthening effect of chitosan.
33812591	5	70	contain	had	1022:1024	arg2	structure					1067:1075	a homogeneous and compact honeycomb-like structure	1026:1075	a homogeneous and compact honeycomb-like structure	1026:1075	Moreover, the scaffolds containing 2% (m/v) collagen and 2% (m/v) chitosan had a homogeneous and compact honeycomb-like structure, demonstrating the strengthening effect of chitosan.
33812591	6	71	dep	meanwhile	1211:1219	arg1	live					1222:1225	live	1222:1225	live	1222:1225	Cell viability assay presented vigorous cell growth on the surface of scaffolds, meanwhile, live cells were also found inside and at the bottom of the scaffolds, indicating the migration of cells.
33812591	7	72	from	applications	1509:1520	arg1	engineering					1532:1542	tissue engineering	1525:1542	tissue engineering	1525:1542	Therefore, chitosan can improve the printability of collagen and the hybrid collagen/chitosan bioinks can be printed into scaffolds with regulated properties, thus can fit different applications in tissue engineering.
33812591	2	73	theme	tissue	247:252	arg1	engineering					254:264	tissue engineering	247:264	tissue engineering scaffold but rarely printed due to their poor printability	247:323	Collagen and chitosan composites are superior materials for tissue engineering scaffold but rarely printed due to their poor printability.
33812591	7	74	theme	tissue	1525:1530	arg1	engineering					1532:1542	tissue engineering	1525:1542	tissue engineering	1525:1542	Therefore, chitosan can improve the printability of collagen and the hybrid collagen/chitosan bioinks can be printed into scaffolds with regulated properties, thus can fit different applications in tissue engineering.
33812591	3	75	theme	bioinks	389:395	arg1	series					346:351	a series	344:351	a series of tunable hybrid collagen/chitosan bioinks with significantly improved printability	344:436	Here, we prepared a series of tunable hybrid collagen/chitosan bioinks with significantly improved printability through hydrogen bond interaction and printed them into scaffolds by carefully controlling the temperature.
32718592	5	0	theme	diatoms	756:762	arg1	shells					746:751	the silica shells	735:751	the silica shells of diatoms, unicellular microalgae with cytocompatible features	735:815	Moreover, resveratrol, a phytoalexin with powerful antioxidant activity, was loaded into the silica shells of diatoms, unicellular microalgae with cytocompatible features.
32718592	7	1	theme	oxidative	1034:1042	arg1	damage					1051:1056	oxidative stress damage	1034:1056	oxidative stress damage	1034:1056	Furthermore, they allowed the controlled release of resveratrol, hindering bacterial proliferation and oxidative stress damage, while supporting stem cell colonization and chondrogenic differentiation.
32718592	1	2	theme	porous	163:168	arg1	biomaterials					194:205	promising biomaterials	184:205	promising biomaterials	184:205	Carbohydrate-based porous scaffolds are promising biomaterials to support cartilage regeneration.
32718592	1	2	theme	porous	163:168	arg1	scaffolds					170:178	Carbohydrate-based porous scaffolds	144:178	Carbohydrate-based porous scaffolds	144:178	Carbohydrate-based porous scaffolds are promising biomaterials to support cartilage regeneration.
32718592	4	3	theme	beehive	576:582	arg1	product					584:590	a beehive product	574:590	a beehive product (Manuka honey)	574:605	Indeed, gellan gum, a bacterial-derived carbohydrate, was blended with a beehive product (Manuka honey) with prominent antibacterial features.
32718592	4	3	theme	beehive	576:582	arg1	honey					600:604	Manuka honey	593:604	Manuka honey	593:604	Indeed, gellan gum, a bacterial-derived carbohydrate, was blended with a beehive product (Manuka honey) with prominent antibacterial features.
32718592	6	4	theme	developed	822:830	arg1	scaffolds					849:857	The developed composite porous scaffolds	818:857	The developed composite porous scaffolds	818:857	The developed composite porous scaffolds demonstrated mechanical properties suitable for cartilage regeneration.
32718592	6	5	theme	mechanical	872:881	arg1	properties					883:892	mechanical properties	872:892	mechanical properties suitable for cartilage regeneration	872:928	The developed composite porous scaffolds demonstrated mechanical properties suitable for cartilage regeneration.
32718592	7	6	dep	cell	1081:1084	arg1	colonization					1086:1097	colonization	1086:1097	colonization	1086:1097	Furthermore, they allowed the controlled release of resveratrol, hindering bacterial proliferation and oxidative stress damage, while supporting stem cell colonization and chondrogenic differentiation.
32718592	4	7	theme	gellan	511:516	arg1	carbohydrate					543:554	a bacterial-derived carbohydrate	523:554	a bacterial-derived carbohydrate	523:554	Indeed, gellan gum, a bacterial-derived carbohydrate, was blended with a beehive product (Manuka honey) with prominent antibacterial features.
32718592	4	7	theme	gellan	511:516	arg1	gum					518:520	gellan gum	511:520	gellan gum	511:520	Indeed, gellan gum, a bacterial-derived carbohydrate, was blended with a beehive product (Manuka honey) with prominent antibacterial features.
32718592	2	8	theme	clinical	303:310	arg1	challenges					312:321	clinical challenges	303:321	clinical challenges	303:321	In this respect, their composition could be designed to face clinical challenges, i.e., articular load bearing, infections and oxidative stress.
32718592	4	9	theme	prominent	612:620	arg1	features					636:643	prominent antibacterial features	612:643	prominent antibacterial features	612:643	Indeed, gellan gum, a bacterial-derived carbohydrate, was blended with a beehive product (Manuka honey) with prominent antibacterial features.
32718592	4	10	link	bacterial-derived	525:541	arg1	carbohydrate					543:554	a bacterial-derived carbohydrate	523:554	a bacterial-derived carbohydrate	523:554	Indeed, gellan gum, a bacterial-derived carbohydrate, was blended with a beehive product (Manuka honey) with prominent antibacterial features.
32718592	4	10	link	bacterial-derived	525:541	arg1	gum					518:520	gellan gum	511:520	gellan gum	511:520	Indeed, gellan gum, a bacterial-derived carbohydrate, was blended with a beehive product (Manuka honey) with prominent antibacterial features.
32718592	3	11	theme	different	475:483	arg1	kingdoms					485:492	different kingdoms	475:492	different kingdoms of life	475:500	Herein, an innovative scaffold has been developed, combining raw materials belonging to different kingdoms of life.
32718592	0	12	theme	cartilage	86:94	arg1	regeneration					96:107	cartilage regeneration	86:107	cartilage regeneration	86:107	From the sea to the bee: Gellan gum-honey-diatom composite to deliver resveratrol for cartilage regeneration under oxidative stress conditions.
32718592	5	13	theme	unicellular	765:775	arg1	diatoms					756:762	diatoms	756:762	diatoms	756:762	Moreover, resveratrol, a phytoalexin with powerful antioxidant activity, was loaded into the silica shells of diatoms, unicellular microalgae with cytocompatible features.
32718592	5	13	theme	unicellular	765:775	arg1	microalgae					777:786	unicellular microalgae	765:786	unicellular microalgae with cytocompatible features	765:815	Moreover, resveratrol, a phytoalexin with powerful antioxidant activity, was loaded into the silica shells of diatoms, unicellular microalgae with cytocompatible features.
32718592	5	14	with	microalgae	777:786	arg1	features					808:815	cytocompatible features	793:815	cytocompatible features	793:815	Moreover, resveratrol, a phytoalexin with powerful antioxidant activity, was loaded into the silica shells of diatoms, unicellular microalgae with cytocompatible features.
32718592	1	15	theme	promising	184:192	arg1	biomaterials					194:205	promising biomaterials	184:205	promising biomaterials	184:205	Carbohydrate-based porous scaffolds are promising biomaterials to support cartilage regeneration.
32718592	1	15	theme	promising	184:192	arg1	scaffolds					170:178	Carbohydrate-based porous scaffolds	144:178	Carbohydrate-based porous scaffolds	144:178	Carbohydrate-based porous scaffolds are promising biomaterials to support cartilage regeneration.
32718592	6	16	theme	cartilage	907:915	arg1	regeneration					917:928	cartilage regeneration	907:928	cartilage regeneration	907:928	The developed composite porous scaffolds demonstrated mechanical properties suitable for cartilage regeneration.
32718592	7	17	theme	resveratrol	983:993	arg1	release					972:978	the controlled release	957:978	the controlled release of resveratrol	957:993	Furthermore, they allowed the controlled release of resveratrol, hindering bacterial proliferation and oxidative stress damage, while supporting stem cell colonization and chondrogenic differentiation.
32718592	7	18	theme	chondrogenic	1103:1114	arg1	differentiation					1116:1130	chondrogenic differentiation	1103:1130	chondrogenic differentiation	1103:1130	Furthermore, they allowed the controlled release of resveratrol, hindering bacterial proliferation and oxidative stress damage, while supporting stem cell colonization and chondrogenic differentiation.
32718592	7	19	theme	controlled	961:970	arg1	release					972:978	the controlled release	957:978	the controlled release of resveratrol	957:993	Furthermore, they allowed the controlled release of resveratrol, hindering bacterial proliferation and oxidative stress damage, while supporting stem cell colonization and chondrogenic differentiation.
32718592	5	20	with	phytoalexin	671:681	arg1	activity					709:716	powerful antioxidant activity	688:716	powerful antioxidant activity	688:716	Moreover, resveratrol, a phytoalexin with powerful antioxidant activity, was loaded into the silica shells of diatoms, unicellular microalgae with cytocompatible features.
32718592	0	21	theme	oxidative	115:123	arg1	conditions					132:141	oxidative stress conditions	115:141	oxidative stress conditions	115:141	From the sea to the bee: Gellan gum-honey-diatom composite to deliver resveratrol for cartilage regeneration under oxidative stress conditions.
32718592	7	22	theme	bacterial	1006:1014	arg1	proliferation					1016:1028	bacterial proliferation	1006:1028	bacterial proliferation	1006:1028	Furthermore, they allowed the controlled release of resveratrol, hindering bacterial proliferation and oxidative stress damage, while supporting stem cell colonization and chondrogenic differentiation.
32718592	4	23	theme	antibacterial	622:634	arg1	features					636:643	prominent antibacterial features	612:643	prominent antibacterial features	612:643	Indeed, gellan gum, a bacterial-derived carbohydrate, was blended with a beehive product (Manuka honey) with prominent antibacterial features.
32718592	2	24	dep	bearing	345:351	arg1	i.e.					324:327	i.e.	324:327	i.e.	324:327	In this respect, their composition could be designed to face clinical challenges, i.e., articular load bearing, infections and oxidative stress.
32718592	1	25	theme	cartilage	218:226	arg1	regeneration					228:239	cartilage regeneration	218:239	cartilage regeneration	218:239	Carbohydrate-based porous scaffolds are promising biomaterials to support cartilage regeneration.
32718592	0	26	theme	bee	20:22	arg1	composite					49:57	the bee: Gellan gum-honey-diatom composite	16:57	the bee: Gellan gum-honey-diatom composite	16:57	From the sea to the bee: Gellan gum-honey-diatom composite to deliver resveratrol for cartilage regeneration under oxidative stress conditions.
32718592	6	27	theme	suitable	894:901	arg1	properties					883:892	mechanical properties	872:892	mechanical properties suitable for cartilage regeneration	872:928	The developed composite porous scaffolds demonstrated mechanical properties suitable for cartilage regeneration.
32718592	0	28	theme	stress	125:130	arg1	conditions					132:141	oxidative stress conditions	115:141	oxidative stress conditions	115:141	From the sea to the bee: Gellan gum-honey-diatom composite to deliver resveratrol for cartilage regeneration under oxidative stress conditions.
32718592	5	29	theme	silica	739:744	arg1	shells					746:751	the silica shells	735:751	the silica shells of diatoms, unicellular microalgae with cytocompatible features	735:815	Moreover, resveratrol, a phytoalexin with powerful antioxidant activity, was loaded into the silica shells of diatoms, unicellular microalgae with cytocompatible features.
32718592	3	30	theme	innovative	398:407	arg1	scaffold					409:416	an innovative scaffold	395:416	an innovative scaffold	395:416	Herein, an innovative scaffold has been developed, combining raw materials belonging to different kingdoms of life.
32718592	0	31	theme	gum-honey-diatom	32:47	arg1	composite					49:57	the bee: Gellan gum-honey-diatom composite	16:57	the bee: Gellan gum-honey-diatom composite	16:57	From the sea to the bee: Gellan gum-honey-diatom composite to deliver resveratrol for cartilage regeneration under oxidative stress conditions.
32718592	7	32	theme	stem	1076:1079	arg1	cell					1081:1084	stem cell colonization and chondrogenic differentiation	1076:1130	cell	1081:1084	Furthermore, they allowed the controlled release of resveratrol, hindering bacterial proliferation and oxidative stress damage, while supporting stem cell colonization and chondrogenic differentiation.
32718592	3	33	theme	life	497:500	arg1	kingdoms					485:492	different kingdoms	475:492	different kingdoms of life	475:500	Herein, an innovative scaffold has been developed, combining raw materials belonging to different kingdoms of life.
32718592	5	34	theme	cytocompatible	793:806	arg1	features					808:815	cytocompatible features	793:815	cytocompatible features	793:815	Moreover, resveratrol, a phytoalexin with powerful antioxidant activity, was loaded into the silica shells of diatoms, unicellular microalgae with cytocompatible features.
32718592	5	35	theme	antioxidant	697:707	arg1	activity					709:716	powerful antioxidant activity	688:716	powerful antioxidant activity	688:716	Moreover, resveratrol, a phytoalexin with powerful antioxidant activity, was loaded into the silica shells of diatoms, unicellular microalgae with cytocompatible features.
32718592	1	36	theme	Carbohydrate-based	144:161	arg1	biomaterials					194:205	promising biomaterials	184:205	promising biomaterials	184:205	Carbohydrate-based porous scaffolds are promising biomaterials to support cartilage regeneration.
32718592	1	36	theme	Carbohydrate-based	144:161	arg1	scaffolds					170:178	Carbohydrate-based porous scaffolds	144:178	Carbohydrate-based porous scaffolds	144:178	Carbohydrate-based porous scaffolds are promising biomaterials to support cartilage regeneration.
32718592	4	37	theme	bacterial-derived	525:541	arg1	carbohydrate					543:554	a bacterial-derived carbohydrate	523:554	a bacterial-derived carbohydrate	523:554	Indeed, gellan gum, a bacterial-derived carbohydrate, was blended with a beehive product (Manuka honey) with prominent antibacterial features.
32718592	4	37	theme	bacterial-derived	525:541	arg1	gum					518:520	gellan gum	511:520	gellan gum	511:520	Indeed, gellan gum, a bacterial-derived carbohydrate, was blended with a beehive product (Manuka honey) with prominent antibacterial features.
32718592	2	38	theme	load	340:343	arg1	bearing					345:351	articular load bearing	330:351	articular load bearing	330:351	In this respect, their composition could be designed to face clinical challenges, i.e., articular load bearing, infections and oxidative stress.
32718592	5	39	theme	powerful	688:695	arg1	activity					709:716	powerful antioxidant activity	688:716	powerful antioxidant activity	688:716	Moreover, resveratrol, a phytoalexin with powerful antioxidant activity, was loaded into the silica shells of diatoms, unicellular microalgae with cytocompatible features.
32718592	6	40	theme	porous	842:847	arg1	scaffolds					849:857	The developed composite porous scaffolds	818:857	The developed composite porous scaffolds	818:857	The developed composite porous scaffolds demonstrated mechanical properties suitable for cartilage regeneration.
32718592	7	41	theme	stress	1044:1049	arg1	damage					1051:1056	oxidative stress damage	1034:1056	oxidative stress damage	1034:1056	Furthermore, they allowed the controlled release of resveratrol, hindering bacterial proliferation and oxidative stress damage, while supporting stem cell colonization and chondrogenic differentiation.
32718592	2	42	theme	articular	330:338	arg1	bearing					345:351	articular load bearing	330:351	articular load bearing	330:351	In this respect, their composition could be designed to face clinical challenges, i.e., articular load bearing, infections and oxidative stress.
32718592	4	43	theme	Manuka	593:598	arg1	product					584:590	a beehive product	574:590	a beehive product (Manuka honey)	574:605	Indeed, gellan gum, a bacterial-derived carbohydrate, was blended with a beehive product (Manuka honey) with prominent antibacterial features.
32718592	4	43	theme	Manuka	593:598	arg1	honey					600:604	Manuka honey	593:604	Manuka honey	593:604	Indeed, gellan gum, a bacterial-derived carbohydrate, was blended with a beehive product (Manuka honey) with prominent antibacterial features.
32718592	6	44	theme	composite	832:840	arg1	scaffolds					849:857	The developed composite porous scaffolds	818:857	The developed composite porous scaffolds	818:857	The developed composite porous scaffolds demonstrated mechanical properties suitable for cartilage regeneration.
32718592	3	45	theme	raw	448:450	arg1	materials					452:460	raw materials	448:460	raw materials belonging to different kingdoms of life	448:500	Herein, an innovative scaffold has been developed, combining raw materials belonging to different kingdoms of life.
32718592	0	46	theme	Gellan	25:30	arg1	composite					49:57	the bee: Gellan gum-honey-diatom composite	16:57	the bee: Gellan gum-honey-diatom composite	16:57	From the sea to the bee: Gellan gum-honey-diatom composite to deliver resveratrol for cartilage regeneration under oxidative stress conditions.
32718592	2	47	theme	oxidative	369:377	arg1	stress					379:384	oxidative stress	369:384	oxidative stress	369:384	In this respect, their composition could be designed to face clinical challenges, i.e., articular load bearing, infections and oxidative stress.
33682214	7	0	theme	predicted	1385:1393	arg1	pGI					1412:1414	pGI	1412:1414	pGI	1412:1414	The principle component analysis (PCA) showed that predicted glycaemic index (pGI) was positively related to A_1022, glucose and rapidly (RDS) content and negatively related to A_995, A_1047, R (1047/1022), resistant starch (RS) and amylose content.
33682214	7	0	theme	predicted	1385:1393	arg1	index					1405:1409	predicted glycaemic index	1385:1409	predicted glycaemic index (pGI)	1385:1415	The principle component analysis (PCA) showed that predicted glycaemic index (pGI) was positively related to A_1022, glucose and rapidly (RDS) content and negatively related to A_995, A_1047, R (1047/1022), resistant starch (RS) and amylose content.
33682214	2	1	theme	starch	573:578	arg1	digestion					580:588	starch digestion	573:588	starch digestion	573:588	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	11	2	theme	glucose	2156:2162	arg1	digestion					2127:2135	starch digestion	2120:2135	starch digestion	2120:2135	Combination of different feedstuffs particular sorghum with corn has interactive effect on starch digestion and the release of glucose.
33682214	11	2	theme	glucose	2156:2162	arg1	release					2145:2151	the release	2141:2151	the release of glucose	2141:2162	Combination of different feedstuffs particular sorghum with corn has interactive effect on starch digestion and the release of glucose.
33682214	3	3	theme	inherent	644:651	arg1	structure					663:671	inherent molecular structure	644:671	inherent molecular structure	644:671	In this study, we determined the inherent molecular structure and granular structure of starch and chemical compositions of seven starchy feedstuffs, as well as starch digestion in single feedstuff and different feedstuffs combined with corn.
33682214	2	4	theme	basic	307:311	arg1	properties					329:338	basic physicochemical properties	307:338	basic physicochemical properties	307:338	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	2	5	dep	explore	507:513	arg1	b					501:501	b	501:501	b	501:501	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	2	6	theme	feedstuffs	559:568	arg1	sources					540:546	different sources	530:546	different sources of starchy feedstuffs	530:568	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	9	7	theme	corn	1713:1716	arg1	combination					1718:1728	corn combination	1713:1728	corn combination	1713:1728	In addition, different starchy feeds and corn combination changed the rate of starch digestion, when barley, however, sorghum combined with corn seemed to affect rate of starch digestion.
33682214	10	8	theme	physicochemical	1907:1921	arg1	properties					1938:1947	basic physicochemical and structural properties	1901:1947	basic physicochemical and structural properties	1901:1947	To sum up, different sources differed in basic physicochemical and structural properties, which would influence the digestion rate of starch and the release of glucose.
33682214	5	9	dep	infrared	1014:1021	arg1	FTIR					1024:1027	FTIR	1024:1027	FTIR	1024:1027	Fourier transforms infrared (FTIR) spectra for barley and wheat had lower (p < 0.05) absorbance band at areas A_860 and A_928 than other feedstuffs, yet rice starch had the lowest value for ratio (R) (1047/1022).
33682214	3	10	theme	granular	677:684	arg1	structure					686:694	granular structure	677:694	granular structure	677:694	In this study, we determined the inherent molecular structure and granular structure of starch and chemical compositions of seven starchy feedstuffs, as well as starch digestion in single feedstuff and different feedstuffs combined with corn.
33682214	2	11	theme	sources	540:546	arg1	effects					519:525	the effects	515:525	the effects of different sources of starchy feedstuffs on starch digestion and glucose release	515:608	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	5	12	dep	Fourier	995:1001	arg1	transforms					1003:1012	transforms	1003:1012	transforms infrared (FTIR) spectra for barley and wheat	1003:1057	Fourier transforms infrared (FTIR) spectra for barley and wheat had lower (p < 0.05) absorbance band at areas A_860 and A_928 than other feedstuffs, yet rice starch had the lowest value for ratio (R) (1047/1022).
33682214	3	13	from	structure	686:694	arg1	feedstuffs					823:832	different feedstuffs	813:832	different feedstuffs combined with corn	813:851	In this study, we determined the inherent molecular structure and granular structure of starch and chemical compositions of seven starchy feedstuffs, as well as starch digestion in single feedstuff and different feedstuffs combined with corn.
33682214	3	13	from	structure	686:694	arg1	feedstuff					799:807	single feedstuff	792:807	single feedstuff	792:807	In this study, we determined the inherent molecular structure and granular structure of starch and chemical compositions of seven starchy feedstuffs, as well as starch digestion in single feedstuff and different feedstuffs combined with corn.
33682214	3	14	theme	compositions	719:730	arg1	structure					663:671	inherent molecular structure	644:671	inherent molecular structure	644:671	In this study, we determined the inherent molecular structure and granular structure of starch and chemical compositions of seven starchy feedstuffs, as well as starch digestion in single feedstuff and different feedstuffs combined with corn.
33682214	3	14	theme	compositions	719:730	arg1	structure					686:694	granular structure	677:694	granular structure	677:694	In this study, we determined the inherent molecular structure and granular structure of starch and chemical compositions of seven starchy feedstuffs, as well as starch digestion in single feedstuff and different feedstuffs combined with corn.
33682214	3	14	theme	compositions	719:730	arg1	digestion					779:787	starch digestion	772:787	starch digestion in single feedstuff and different feedstuffs combined with corn	772:851	In this study, we determined the inherent molecular structure and granular structure of starch and chemical compositions of seven starchy feedstuffs, as well as starch digestion in single feedstuff and different feedstuffs combined with corn.
33682214	9	15	theme	starch	1750:1755	arg1	digestion					1757:1765	starch digestion	1750:1765	starch digestion	1750:1765	In addition, different starchy feeds and corn combination changed the rate of starch digestion, when barley, however, sorghum combined with corn seemed to affect rate of starch digestion.
33682214	1	16	theme	largest	197:203	arg1	Starch					183:188	Starch	183:188	Starch	183:188	Starch is the largest constituent in animal diets.
33682214	1	16	theme	largest	197:203	arg1	constituent					205:215	the largest constituent	193:215	the largest constituent in animal diets	193:231	Starch is the largest constituent in animal diets.
33682214	0	17	theme	structural	31:40	arg1	properties					42:51	physicochemical and structural properties	11:51	physicochemical and structural properties of single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics	11:180	Effects of physicochemical and structural properties of single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics.
33682214	4	18	theme	significant	906:916	arg1	difference					918:927	significant difference	906:927	significant difference between granule shape and size of starch of different feedstuffs	906:992	Scanning electron microscope (SEM) results revealed significant difference between granule shape and size of starch of different feedstuffs.
33682214	10	19	theme	structural	1927:1936	arg1	properties					1938:1947	basic physicochemical and structural properties	1901:1947	basic physicochemical and structural properties	1901:1947	To sum up, different sources differed in basic physicochemical and structural properties, which would influence the digestion rate of starch and the release of glucose.
33682214	3	20	theme	feedstuffs	749:758	arg1	compositions					719:730	starch and chemical compositions	699:730	starch and chemical compositions of seven starchy feedstuffs	699:758	In this study, we determined the inherent molecular structure and granular structure of starch and chemical compositions of seven starchy feedstuffs, as well as starch digestion in single feedstuff and different feedstuffs combined with corn.
33682214	1	21	from	constituent	205:215	arg1	diets					227:231	animal diets	220:231	animal diets	220:231	Starch is the largest constituent in animal diets.
33682214	4	22	theme	microscope	872:881	arg1	results					889:895	Scanning electron microscope (SEM) results	854:895	Scanning electron microscope (SEM) results	854:895	Scanning electron microscope (SEM) results revealed significant difference between granule shape and size of starch of different feedstuffs.
33682214	0	23	theme	release	165:171	arg1	kinetics					173:180	glucose release kinetics	157:180	glucose release kinetics	157:180	Effects of physicochemical and structural properties of single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics.
33682214	4	24	theme	Scanning	854:861	arg1	SEM					884:886	SEM	884:886	SEM	884:886	Scanning electron microscope (SEM) results revealed significant difference between granule shape and size of starch of different feedstuffs.
33682214	4	24	theme	Scanning	854:861	arg1	microscope					872:881	Scanning electron microscope	854:881	Scanning electron microscope (SEM) results	854:895	Scanning electron microscope (SEM) results revealed significant difference between granule shape and size of starch of different feedstuffs.
33682214	2	25	theme	different	530:538	arg1	sources					540:546	different sources	530:546	different sources of starchy feedstuffs	530:568	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	0	26	theme	glucose	157:163	arg1	kinetics					173:180	glucose release kinetics	157:180	glucose release kinetics	157:180	Effects of physicochemical and structural properties of single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics.
33682214	8	27	theme	corn	1612:1615	arg1	combination					1617:1627	corn combination	1612:1627	corn combination	1612:1627	Most of the feedstufss with corn combination had no effect on rate of starch digestion.
33682214	10	28	theme	starch	1994:1999	arg1	release					2009:2015	the release	2005:2015	the release of glucose	2005:2026	To sum up, different sources differed in basic physicochemical and structural properties, which would influence the digestion rate of starch and the release of glucose.
33682214	10	28	theme	starch	1994:1999	arg1	rate					1986:1989	the digestion rate	1972:1989	the digestion rate of starch	1972:1999	To sum up, different sources differed in basic physicochemical and structural properties, which would influence the digestion rate of starch and the release of glucose.
33682214	5	29	theme	absorbance	1080:1089	arg1	band					1091:1094	lower (p < 0.05) absorbance band	1063:1094	lower (p < 0.05) absorbance band at areas A_860 and A_928	1063:1119	Fourier transforms infrared (FTIR) spectra for barley and wheat had lower (p < 0.05) absorbance band at areas A_860 and A_928 than other feedstuffs, yet rice starch had the lowest value for ratio (R) (1047/1022).
33682214	4	30	theme	starch	963:968	arg1	size					955:958	size	955:958	size	955:958	Scanning electron microscope (SEM) results revealed significant difference between granule shape and size of starch of different feedstuffs.
33682214	4	30	theme	starch	963:968	arg1	shape					945:949	granule shape	937:949	granule shape	937:949	Scanning electron microscope (SEM) results revealed significant difference between granule shape and size of starch of different feedstuffs.
33682214	3	31	theme	single	792:797	arg1	feedstuff					799:807	single feedstuff	792:807	single feedstuff	792:807	In this study, we determined the inherent molecular structure and granular structure of starch and chemical compositions of seven starchy feedstuffs, as well as starch digestion in single feedstuff and different feedstuffs combined with corn.
33682214	0	32	theme	feedstuffs	74:83	arg1	properties					42:51	physicochemical and structural properties	11:51	physicochemical and structural properties of single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics	11:180	Effects of physicochemical and structural properties of single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics.
33682214	5	33	theme	lower	1063:1067	arg1	band					1091:1094	lower (p < 0.05) absorbance band	1063:1094	lower (p < 0.05) absorbance band at areas A_860 and A_928	1063:1119	Fourier transforms infrared (FTIR) spectra for barley and wheat had lower (p < 0.05) absorbance band at areas A_860 and A_928 than other feedstuffs, yet rice starch had the lowest value for ratio (R) (1047/1022).
33682214	2	34	theme	digestion	360:368	arg1	variability					292:302	the variability	288:302	the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs	288:390	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	0	35	theme	botanic	108:114	arg1	sources					116:122	different botanic sources	98:122	different botanic sources	98:122	Effects of physicochemical and structural properties of single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics.
33682214	8	36	theme	starch	1654:1659	arg1	digestion					1661:1669	starch digestion	1654:1669	starch digestion	1654:1669	Most of the feedstufss with corn combination had no effect on rate of starch digestion.
33682214	2	37	theme	physicochemical	429:443	arg1	properties					445:454	physicochemical properties	429:454	physicochemical properties	429:454	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	5	38	theme	other	1126:1130	arg1	feedstuffs					1132:1141	other feedstuffs	1126:1141	other feedstuffs	1126:1141	Fourier transforms infrared (FTIR) spectra for barley and wheat had lower (p < 0.05) absorbance band at areas A_860 and A_928 than other feedstuffs, yet rice starch had the lowest value for ratio (R) (1047/1022).
33682214	9	39	dep	starchy	1695:1701	arg1	feeds					1703:1707	feeds	1703:1707	feeds	1703:1707	In addition, different starchy feeds and corn combination changed the rate of starch digestion, when barley, however, sorghum combined with corn seemed to affect rate of starch digestion.
33682214	3	40	from	digestion	779:787	arg1	feedstuffs					823:832	different feedstuffs	813:832	different feedstuffs combined with corn	813:851	In this study, we determined the inherent molecular structure and granular structure of starch and chemical compositions of seven starchy feedstuffs, as well as starch digestion in single feedstuff and different feedstuffs combined with corn.
33682214	3	40	from	digestion	779:787	arg1	feedstuff					799:807	single feedstuff	792:807	single feedstuff	792:807	In this study, we determined the inherent molecular structure and granular structure of starch and chemical compositions of seven starchy feedstuffs, as well as starch digestion in single feedstuff and different feedstuffs combined with corn.
33682214	9	41	theme	digestion	1849:1857	arg1	rate					1834:1837	rate	1834:1837	rate of starch digestion	1834:1857	In addition, different starchy feeds and corn combination changed the rate of starch digestion, when barley, however, sorghum combined with corn seemed to affect rate of starch digestion.
33682214	0	42	theme	physicochemical	11:25	arg1	properties					42:51	physicochemical and structural properties	11:51	physicochemical and structural properties of single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics	11:180	Effects of physicochemical and structural properties of single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics.
33682214	3	43	dep	structure	663:671	arg1	the					640:642	the	640:642	the	640:642	In this study, we determined the inherent molecular structure and granular structure of starch and chemical compositions of seven starchy feedstuffs, as well as starch digestion in single feedstuff and different feedstuffs combined with corn.
33682214	4	44	theme	different	973:981	arg1	feedstuffs					983:992	different feedstuffs	973:992	different feedstuffs	973:992	Scanning electron microscope (SEM) results revealed significant difference between granule shape and size of starch of different feedstuffs.
33682214	3	45	theme	different	813:821	arg1	feedstuffs					823:832	different feedstuffs	813:832	different feedstuffs combined with corn	813:851	In this study, we determined the inherent molecular structure and granular structure of starch and chemical compositions of seven starchy feedstuffs, as well as starch digestion in single feedstuff and different feedstuffs combined with corn.
33682214	2	46	theme	starchy	373:379	arg1	feedstuffs					381:390	starchy feedstuffs	373:390	starchy feedstuffs	373:390	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	6	47	theme	broken	1320:1325	arg1	starch					1312:1317	rapidly starch	1304:1317	rapidly starch (broken rice)	1304:1331	Moreover, digestion rate ranged from 0.0157/min for resistant starch (sorghum) to 0.029/min for rapidly starch (broken rice).
33682214	6	47	theme	broken	1320:1325	arg1	rice					1327:1330	broken rice	1320:1330	broken rice	1320:1330	Moreover, digestion rate ranged from 0.0157/min for resistant starch (sorghum) to 0.029/min for rapidly starch (broken rice).
33682214	5	48	from	areas	1099:1103	arg1	band					1091:1094	lower (p < 0.05) absorbance band	1063:1094	lower (p < 0.05) absorbance band at areas A_860 and A_928	1063:1119	Fourier transforms infrared (FTIR) spectra for barley and wheat had lower (p < 0.05) absorbance band at areas A_860 and A_928 than other feedstuffs, yet rice starch had the lowest value for ratio (R) (1047/1022).
33682214	5	49	contain	had	1160:1162	arg2	value					1175:1179	the lowest value	1164:1179	the lowest value for ratio (R) (1047/1022)	1164:1205	Fourier transforms infrared (FTIR) spectra for barley and wheat had lower (p < 0.05) absorbance band at areas A_860 and A_928 than other feedstuffs, yet rice starch had the lowest value for ratio (R) (1047/1022).
33682214	5	49	contain	had	1160:1162	arg1	starch					1153:1158	rice starch	1148:1158	rice starch	1148:1158	Fourier transforms infrared (FTIR) spectra for barley and wheat had lower (p < 0.05) absorbance band at areas A_860 and A_928 than other feedstuffs, yet rice starch had the lowest value for ratio (R) (1047/1022).
33682214	5	50	theme	lowest	1168:1173	arg1	value					1175:1179	the lowest value	1164:1179	the lowest value for ratio (R) (1047/1022)	1164:1205	Fourier transforms infrared (FTIR) spectra for barley and wheat had lower (p < 0.05) absorbance band at areas A_860 and A_928 than other feedstuffs, yet rice starch had the lowest value for ratio (R) (1047/1022).
33682214	7	51	theme	glycaemic	1395:1403	arg1	pGI					1412:1414	pGI	1412:1414	pGI	1412:1414	The principle component analysis (PCA) showed that predicted glycaemic index (pGI) was positively related to A_1022, glucose and rapidly (RDS) content and negatively related to A_995, A_1047, R (1047/1022), resistant starch (RS) and amylose content.
33682214	7	51	theme	glycaemic	1395:1403	arg1	index					1405:1409	predicted glycaemic index	1385:1409	predicted glycaemic index (pGI)	1385:1415	The principle component analysis (PCA) showed that predicted glycaemic index (pGI) was positively related to A_1022, glucose and rapidly (RDS) content and negatively related to A_995, A_1047, R (1047/1022), resistant starch (RS) and amylose content.
33682214	2	52	dep	were	257:260	arg1	a					275:275	a	275:275	a	275:275	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	11	53	theme	feedstuffs	2054:2063	arg1	sorghum					2076:2082	different feedstuffs particular sorghum	2044:2082	different feedstuffs particular sorghum	2044:2082	Combination of different feedstuffs particular sorghum with corn has interactive effect on starch digestion and the release of glucose.
33682214	5	54	from	A_928	1115:1119	arg1	band					1091:1094	lower (p < 0.05) absorbance band	1063:1094	lower (p < 0.05) absorbance band at areas A_860 and A_928	1063:1119	Fourier transforms infrared (FTIR) spectra for barley and wheat had lower (p < 0.05) absorbance band at areas A_860 and A_928 than other feedstuffs, yet rice starch had the lowest value for ratio (R) (1047/1022).
33682214	2	55	theme	physicochemical	313:327	arg1	properties					329:338	basic physicochemical properties	307:338	basic physicochemical properties	307:338	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	5	56	theme	rice	1148:1151	arg1	starch					1153:1158	rice starch	1148:1158	rice starch	1148:1158	Fourier transforms infrared (FTIR) spectra for barley and wheat had lower (p < 0.05) absorbance band at areas A_860 and A_928 than other feedstuffs, yet rice starch had the lowest value for ratio (R) (1047/1022).
33682214	10	57	theme	different	1871:1879	arg1	sources					1881:1887	different sources	1871:1887	different sources	1871:1887	To sum up, different sources differed in basic physicochemical and structural properties, which would influence the digestion rate of starch and the release of glucose.
33682214	3	58	theme	molecular	653:661	arg1	structure					663:671	inherent molecular structure	644:671	inherent molecular structure	644:671	In this study, we determined the inherent molecular structure and granular structure of starch and chemical compositions of seven starchy feedstuffs, as well as starch digestion in single feedstuff and different feedstuffs combined with corn.
33682214	2	59	theme	starchy	551:557	arg1	feedstuffs					559:568	starchy feedstuffs	551:568	starchy feedstuffs	551:568	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	7	60	theme	principle	1338:1346	arg1	PCA					1368:1370	PCA	1368:1370	PCA	1368:1370	The principle component analysis (PCA) showed that predicted glycaemic index (pGI) was positively related to A_1022, glucose and rapidly (RDS) content and negatively related to A_995, A_1047, R (1047/1022), resistant starch (RS) and amylose content.
33682214	7	60	theme	principle	1338:1346	arg1	analysis					1358:1365	The principle component analysis	1334:1365	The principle component analysis (PCA)	1334:1371	The principle component analysis (PCA) showed that predicted glycaemic index (pGI) was positively related to A_1022, glucose and rapidly (RDS) content and negatively related to A_995, A_1047, R (1047/1022), resistant starch (RS) and amylose content.
33682214	11	61	theme	sorghum	2076:2082	arg1	Combination					2029:2039	Combination	2029:2039	Combination of different feedstuffs particular sorghum with corn	2029:2092	Combination of different feedstuffs particular sorghum with corn has interactive effect on starch digestion and the release of glucose.
33682214	11	62	theme	particular	2065:2074	arg1	sorghum					2076:2082	different feedstuffs particular sorghum	2044:2082	different feedstuffs particular sorghum	2044:2082	Combination of different feedstuffs particular sorghum with corn has interactive effect on starch digestion and the release of glucose.
33682214	3	63	theme	starch	699:704	arg1	compositions					719:730	starch and chemical compositions	699:730	starch and chemical compositions of seven starchy feedstuffs	699:758	In this study, we determined the inherent molecular structure and granular structure of starch and chemical compositions of seven starchy feedstuffs, as well as starch digestion in single feedstuff and different feedstuffs combined with corn.
33682214	7	64	theme	component	1348:1356	arg1	PCA					1368:1370	PCA	1368:1370	PCA	1368:1370	The principle component analysis (PCA) showed that predicted glycaemic index (pGI) was positively related to A_1022, glucose and rapidly (RDS) content and negatively related to A_995, A_1047, R (1047/1022), resistant starch (RS) and amylose content.
33682214	7	64	theme	component	1348:1356	arg1	analysis					1358:1365	The principle component analysis	1334:1365	The principle component analysis (PCA)	1334:1371	The principle component analysis (PCA) showed that predicted glycaemic index (pGI) was positively related to A_1022, glucose and rapidly (RDS) content and negatively related to A_995, A_1047, R (1047/1022), resistant starch (RS) and amylose content.
33682214	2	65	theme	glucose	594:600	arg1	release					602:608	glucose release	594:608	glucose release	594:608	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	10	66	theme	basic	1901:1905	arg1	properties					1938:1947	basic physicochemical and structural properties	1901:1947	basic physicochemical and structural properties	1901:1947	To sum up, different sources differed in basic physicochemical and structural properties, which would influence the digestion rate of starch and the release of glucose.
33682214	8	67	with	feedstufss	1596:1605	arg1	combination					1617:1627	corn combination	1612:1627	corn combination	1612:1627	Most of the feedstufss with corn combination had no effect on rate of starch digestion.
33682214	11	68	theme	interactive	2098:2108	arg1	effect					2110:2115	interactive effect	2098:2115	interactive effect	2098:2115	Combination of different feedstuffs particular sorghum with corn has interactive effect on starch digestion and the release of glucose.
33682214	6	69	theme	resistant	1260:1268	arg1	sorghum					1278:1284	sorghum	1278:1284	sorghum	1278:1284	Moreover, digestion rate ranged from 0.0157/min for resistant starch (sorghum) to 0.029/min for rapidly starch (broken rice).
33682214	6	69	theme	resistant	1260:1268	arg1	starch					1270:1275	resistant starch	1260:1275	resistant starch (sorghum) to 0.029/min for rapidly starch (broken rice)	1260:1331	Moreover, digestion rate ranged from 0.0157/min for resistant starch (sorghum) to 0.029/min for rapidly starch (broken rice).
33682214	9	70	theme	digestion	1757:1765	arg1	rate					1742:1745	the rate	1738:1745	the rate of starch digestion	1738:1765	In addition, different starchy feeds and corn combination changed the rate of starch digestion, when barley, however, sorghum combined with corn seemed to affect rate of starch digestion.
33682214	3	71	theme	chemical	710:717	arg1	compositions					719:730	starch and chemical compositions	699:730	starch and chemical compositions of seven starchy feedstuffs	699:758	In this study, we determined the inherent molecular structure and granular structure of starch and chemical compositions of seven starchy feedstuffs, as well as starch digestion in single feedstuff and different feedstuffs combined with corn.
33682214	0	72	theme	starch	136:141	arg1	digestion					143:151	in vitro starch digestion	127:151	in vitro starch digestion	127:151	Effects of physicochemical and structural properties of single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics.
33682214	11	73	with	Combination	2029:2039	arg1	corn					2089:2092	corn	2089:2092	corn	2089:2092	Combination of different feedstuffs particular sorghum with corn has interactive effect on starch digestion and the release of glucose.
33682214	10	74	theme	digestion	1976:1984	arg1	rate					1986:1989	the digestion rate	1972:1989	the digestion rate of starch	1972:1999	To sum up, different sources differed in basic physicochemical and structural properties, which would influence the digestion rate of starch and the release of glucose.
33682214	3	75	from	structure	663:671	arg1	feedstuffs					823:832	different feedstuffs	813:832	different feedstuffs combined with corn	813:851	In this study, we determined the inherent molecular structure and granular structure of starch and chemical compositions of seven starchy feedstuffs, as well as starch digestion in single feedstuff and different feedstuffs combined with corn.
33682214	3	75	from	structure	663:671	arg1	feedstuff					799:807	single feedstuff	792:807	single feedstuff	792:807	In this study, we determined the inherent molecular structure and granular structure of starch and chemical compositions of seven starchy feedstuffs, as well as starch digestion in single feedstuff and different feedstuffs combined with corn.
33682214	0	76	theme	in	127:128	arg1	digestion					143:151	in vitro starch digestion	127:151	in vitro starch digestion	127:151	Effects of physicochemical and structural properties of single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics.
33682214	4	77	theme	electron	863:870	arg1	SEM					884:886	SEM	884:886	SEM	884:886	Scanning electron microscope (SEM) results revealed significant difference between granule shape and size of starch of different feedstuffs.
33682214	4	77	theme	electron	863:870	arg1	microscope					872:881	Scanning electron microscope	854:881	Scanning electron microscope (SEM) results	854:895	Scanning electron microscope (SEM) results revealed significant difference between granule shape and size of starch of different feedstuffs.
33682214	3	78	theme	starchy	741:747	arg1	feedstuffs					749:758	seven starchy feedstuffs	735:758	seven starchy feedstuffs	735:758	In this study, we determined the inherent molecular structure and granular structure of starch and chemical compositions of seven starchy feedstuffs, as well as starch digestion in single feedstuff and different feedstuffs combined with corn.
33682214	6	79	theme	digestion	1218:1226	arg1	rate					1228:1231	digestion rate	1218:1231	digestion rate	1218:1231	Moreover, digestion rate ranged from 0.0157/min for resistant starch (sorghum) to 0.029/min for rapidly starch (broken rice).
33682214	2	80	theme	study	251:255	arg1	aims					238:241	The aims	234:241	The aims of this study	234:255	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	3	81	theme	starch	772:777	arg1	digestion					779:787	starch digestion	772:787	starch digestion in single feedstuff and different feedstuffs combined with corn	772:851	In this study, we determined the inherent molecular structure and granular structure of starch and chemical compositions of seven starchy feedstuffs, as well as starch digestion in single feedstuff and different feedstuffs combined with corn.
33682214	0	82	theme	double	67:72	arg1	feedstuffs					74:83	single and double feedstuffs	56:83	single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics	56:180	Effects of physicochemical and structural properties of single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics.
33682214	7	83	theme	amylose	1567:1573	arg1	content					1575:1581	amylose content	1567:1581	amylose content	1567:1581	The principle component analysis (PCA) showed that predicted glycaemic index (pGI) was positively related to A_1022, glucose and rapidly (RDS) content and negatively related to A_995, A_1047, R (1047/1022), resistant starch (RS) and amylose content.
33682214	2	84	theme	feedstuffs	484:493	arg1	properties					445:454	physicochemical properties	429:454	physicochemical properties	429:454	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	2	84	theme	feedstuffs	484:493	arg1	digestion					467:475	starch digestion	460:475	starch digestion	460:475	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	11	85	contain	has	2094:2096	arg2	effect					2110:2115	interactive effect	2098:2115	interactive effect	2098:2115	Combination of different feedstuffs particular sorghum with corn has interactive effect on starch digestion and the release of glucose.
33682214	11	85	contain	has	2094:2096	arg1	Combination					2029:2039	Combination	2029:2039	Combination of different feedstuffs particular sorghum with corn	2029:2092	Combination of different feedstuffs particular sorghum with corn has interactive effect on starch digestion and the release of glucose.
33682214	1	86	theme	animal	220:225	arg1	diets					227:231	animal diets	220:231	animal diets	220:231	Starch is the largest constituent in animal diets.
33682214	0	87	dep	in	127:128	arg1	vitro					130:134	vitro	130:134	vitro	130:134	Effects of physicochemical and structural properties of single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics.
33682214	10	88	theme	glucose	2020:2026	arg1	release					2009:2015	the release	2005:2015	the release of glucose	2005:2026	To sum up, different sources differed in basic physicochemical and structural properties, which would influence the digestion rate of starch and the release of glucose.
33682214	10	88	theme	glucose	2020:2026	arg1	rate					1986:1989	the digestion rate	1972:1989	the digestion rate of starch	1972:1999	To sum up, different sources differed in basic physicochemical and structural properties, which would influence the digestion rate of starch and the release of glucose.
33682214	0	89	theme	different	98:106	arg1	sources					116:122	different botanic sources	98:122	different botanic sources	98:122	Effects of physicochemical and structural properties of single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics.
33682214	11	90	theme	different	2044:2052	arg1	sorghum					2076:2082	different feedstuffs particular sorghum	2044:2082	different feedstuffs particular sorghum	2044:2082	Combination of different feedstuffs particular sorghum with corn has interactive effect on starch digestion and the release of glucose.
33682214	7	91	theme	resistant	1541:1549	arg1	RS					1559:1560	RS	1559:1560	RS	1559:1560	The principle component analysis (PCA) showed that predicted glycaemic index (pGI) was positively related to A_1022, glucose and rapidly (RDS) content and negatively related to A_995, A_1047, R (1047/1022), resistant starch (RS) and amylose content.
33682214	7	91	theme	resistant	1541:1549	arg1	starch					1551:1556	resistant starch	1541:1556	resistant starch (RS)	1541:1561	The principle component analysis (PCA) showed that predicted glycaemic index (pGI) was positively related to A_1022, glucose and rapidly (RDS) content and negatively related to A_995, A_1047, R (1047/1022), resistant starch (RS) and amylose content.
33682214	4	92	theme	granule	937:943	arg1	shape					945:949	granule shape	937:949	granule shape	937:949	Scanning electron microscope (SEM) results revealed significant difference between granule shape and size of starch of different feedstuffs.
33682214	2	93	theme	starch	353:358	arg1	digestion					360:368	in vitro starch digestion	344:368	in vitro starch digestion	344:368	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	9	94	theme	starch	1842:1847	arg1	digestion					1849:1857	starch digestion	1842:1857	starch digestion	1842:1857	In addition, different starchy feeds and corn combination changed the rate of starch digestion, when barley, however, sorghum combined with corn seemed to affect rate of starch digestion.
33682214	2	95	theme	in	344:345	arg1	digestion					360:368	in vitro starch digestion	344:368	in vitro starch digestion	344:368	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	8	96	contain	had	1629:1631	arg1	Most					1584:1587	Most	1584:1587	Most	1584:1587	Most of the feedstufss with corn combination had no effect on rate of starch digestion.
33682214	8	96	contain	had	1629:1631	arg1	feedstufss					1596:1605	the feedstufss	1592:1605	the feedstufss with corn combination	1592:1627	Most of the feedstufss with corn combination had no effect on rate of starch digestion.
33682214	8	96	contain	had	1629:1631	arg2	effect					1636:1641	no effect	1633:1641	no effect	1633:1641	Most of the feedstufss with corn combination had no effect on rate of starch digestion.
33682214	8	97	theme	digestion	1661:1669	arg1	rate					1646:1649	rate	1646:1649	rate of starch digestion	1646:1669	Most of the feedstufss with corn combination had no effect on rate of starch digestion.
33682214	2	98	theme	feedstuffs	381:390	arg1	digestion					360:368	in vitro starch digestion	344:368	in vitro starch digestion	344:368	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	2	98	theme	feedstuffs	381:390	arg1	properties					329:338	basic physicochemical properties	307:338	basic physicochemical properties	307:338	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	0	99	theme	properties	42:51	arg1	Effects					0:6	Effects	0:6	Effects of physicochemical and structural properties of single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics.	0:181	Effects of physicochemical and structural properties of single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics.
33682214	11	100	theme	starch	2120:2125	arg1	digestion					2127:2135	starch digestion	2120:2135	starch digestion	2120:2135	Combination of different feedstuffs particular sorghum with corn has interactive effect on starch digestion and the release of glucose.
33682214	2	101	theme	starch	460:465	arg1	digestion					467:475	starch digestion	460:475	starch digestion	460:475	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	0	102	theme	single	56:61	arg1	feedstuffs					74:83	single and double feedstuffs	56:83	single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics	56:180	Effects of physicochemical and structural properties of single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics.
33682214	5	103	theme	infrared	1014:1021	arg1	spectra					1030:1036	infrared (FTIR) spectra	1014:1036	infrared (FTIR) spectra	1014:1036	Fourier transforms infrared (FTIR) spectra for barley and wheat had lower (p < 0.05) absorbance band at areas A_860 and A_928 than other feedstuffs, yet rice starch had the lowest value for ratio (R) (1047/1022).
33682214	4	104	theme	feedstuffs	983:992	arg1	starch					963:968	starch	963:968	starch of different feedstuffs	963:992	Scanning electron microscope (SEM) results revealed significant difference between granule shape and size of starch of different feedstuffs.
33682214	5	105	dep	lower	1063:1067	arg1	p < 0.05					1070:1077	p < 0.05	1070:1077	p < 0.05	1070:1077	Fourier transforms infrared (FTIR) spectra for barley and wheat had lower (p < 0.05) absorbance band at areas A_860 and A_928 than other feedstuffs, yet rice starch had the lowest value for ratio (R) (1047/1022).
33682214	0	106	attach	derived	85:91	arg1	sources					116:122	different botanic sources	98:122	different botanic sources	98:122	Effects of physicochemical and structural properties of single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics.
33682214	0	106	attach	derived	85:91	arg2	feedstuffs					74:83	single and double feedstuffs	56:83	single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics	56:180	Effects of physicochemical and structural properties of single and double feedstuffs derived from different botanic sources on in vitro starch digestion and glucose release kinetics.
33682214	2	107	dep	in	344:345	arg1	vitro					347:351	vitro	347:351	vitro	347:351	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	2	108	theme	properties	329:338	arg1	variability					292:302	the variability	288:302	the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs	288:390	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	9	109	theme	different	1685:1693	arg1	starchy					1695:1701	different starchy feeds	1685:1707	different starchy feeds	1685:1707	In addition, different starchy feeds and corn combination changed the rate of starch digestion, when barley, however, sorghum combined with corn seemed to affect rate of starch digestion.
33682214	2	110	from	effects	519:525	arg1	digestion					580:588	starch digestion	573:588	starch digestion	573:588	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	2	110	from	effects	519:525	arg1	release					602:608	glucose release	594:608	glucose release	594:608	The aims of this study were as follows: (a) to assess the variability of basic physicochemical properties and in vitro starch digestion of starchy feedstuffs and investigate relationship between physicochemical properties and starch digestion of the feedstuffs, and (b) to explore the effects of different sources of starchy feedstuffs on starch digestion and glucose release.
33682214	5	111	contain	had	1059:1061	arg2	band					1091:1094	lower (p < 0.05) absorbance band	1063:1094	lower (p < 0.05) absorbance band at areas A_860 and A_928	1063:1119	Fourier transforms infrared (FTIR) spectra for barley and wheat had lower (p < 0.05) absorbance band at areas A_860 and A_928 than other feedstuffs, yet rice starch had the lowest value for ratio (R) (1047/1022).
33682214	5	111	contain	had	1059:1061	arg1	Fourier					995:1001	Fourier	995:1001	Fourier transforms infrared (FTIR) spectra for barley and wheat	995:1057	Fourier transforms infrared (FTIR) spectra for barley and wheat had lower (p < 0.05) absorbance band at areas A_860 and A_928 than other feedstuffs, yet rice starch had the lowest value for ratio (R) (1047/1022).
33910732	0	0	theme	alcohol	107:113	arg1	composites					129:138	catechol-functionalized chitosan/polyvinyl alcohol biodegradable composites	64:138	catechol-functionalized chitosan/polyvinyl alcohol biodegradable composites for active food packaging	64:164	Improved mechanical, antibacterial and UV barrier properties of catechol-functionalized chitosan/polyvinyl alcohol biodegradable composites for active food packaging.
33910732	3	1	from	range	640:644	arg1	transparent					613:623	transparent	613:623	transparent	613:623	Still, all the C-CS/PVA composite films are transparent in the visible range.
33910732	3	1	from	range	640:644	arg1	films					603:607	all the C-CS/PVA composite films	576:607	all the C-CS/PVA composite films	576:607	Still, all the C-CS/PVA composite films are transparent in the visible range.
33910732	7	2	theme	C-CS/PVA	1064:1071	arg1	films					1083:1087	C-CS/PVA composite films	1064:1087	C-CS/PVA composite films	1064:1087	Therefore, C-CS/PVA composite films show great potential in the field of active packaging due to its good mechanical, antibacterial and UV barrier properties.
33910732	4	3	theme	PVA	825:827	arg1	film					829:832	pure PVA film	820:832	pure PVA film	820:832	The maximal tensile strength and elongation at break of C-CS/PVA composite films can reach 45.2 MPa and 153 % respectively, which are 46.3 % and 25.4 % higher than those of pure PVA film.
33910732	0	4	theme	chitosan/polyvinyl	88:105	arg1	composites					129:138	catechol-functionalized chitosan/polyvinyl alcohol biodegradable composites	64:138	catechol-functionalized chitosan/polyvinyl alcohol biodegradable composites for active food packaging	64:164	Improved mechanical, antibacterial and UV barrier properties of catechol-functionalized chitosan/polyvinyl alcohol biodegradable composites for active food packaging.
33910732	7	5	theme	composite	1073:1081	arg1	films					1083:1087	C-CS/PVA composite films	1064:1087	C-CS/PVA composite films	1064:1087	Therefore, C-CS/PVA composite films show great potential in the field of active packaging due to its good mechanical, antibacterial and UV barrier properties.
33910732	6	6	theme	C-CS	1047:1050	arg1	C-CS					1047:1050	C-CS	1047:1050	C-CS	1047:1050	The water resistance of C-CS/PVA composite films can not be significantly deteriorated by the appropriate amount of C-CS.
33910732	6	6	theme	C-CS	1047:1050	arg1	amount					1037:1042	the appropriate amount	1021:1042	the appropriate amount of C-CS	1021:1050	The water resistance of C-CS/PVA composite films can not be significantly deteriorated by the appropriate amount of C-CS.
33910732	4	7	theme	tensile	659:665	arg1	strength					667:674	maximal tensile strength	651:674	maximal tensile strength	651:674	The maximal tensile strength and elongation at break of C-CS/PVA composite films can reach 45.2 MPa and 153 % respectively, which are 46.3 % and 25.4 % higher than those of pure PVA film.
33910732	1	8	theme	C-CS	293:296	arg1	films					333:337	catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films	259:337	catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films	259:337	Bioinspired from adhesion behaviors of mussels, we first reported a new strategy to prepare catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films via a solution blending method in neutral aqueous solution for active food packaging.
33910732	0	9	theme	composites	129:138	arg1	properties					50:59	mechanical, antibacterial and UV barrier properties	9:59	properties	50:59	Improved mechanical, antibacterial and UV barrier properties of catechol-functionalized chitosan/polyvinyl alcohol biodegradable composites for active food packaging.
33910732	4	10	dep	strength	667:674	arg1	The					647:649	The	647:649	The	647:649	The maximal tensile strength and elongation at break of C-CS/PVA composite films can reach 45.2 MPa and 153 % respectively, which are 46.3 % and 25.4 % higher than those of pure PVA film.
33910732	0	11	theme	biodegradable	115:127	arg1	composites					129:138	catechol-functionalized chitosan/polyvinyl alcohol biodegradable composites	64:138	catechol-functionalized chitosan/polyvinyl alcohol biodegradable composites for active food packaging	64:164	Improved mechanical, antibacterial and UV barrier properties of catechol-functionalized chitosan/polyvinyl alcohol biodegradable composites for active food packaging.
33910732	1	12	theme	neutral	373:379	arg1	solution					389:396	neutral aqueous solution	373:396	neutral aqueous solution	373:396	Bioinspired from adhesion behaviors of mussels, we first reported a new strategy to prepare catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films via a solution blending method in neutral aqueous solution for active food packaging.
33910732	7	13	theme	great	1094:1098	arg1	potential					1100:1108	great potential	1094:1108	great potential	1094:1108	Therefore, C-CS/PVA composite films show great potential in the field of active packaging due to its good mechanical, antibacterial and UV barrier properties.
33910732	5	14	theme	antibacterial	891:903	arg1	properties					905:914	the antibacterial properties	887:914	the antibacterial properties	887:914	The incorporation of C-CS into PVA matrix increases the antibacterial properties significantly.
33910732	1	15	theme	/polyvinyl	298:307	arg1	films					333:337	catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films	259:337	catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films	259:337	Bioinspired from adhesion behaviors of mussels, we first reported a new strategy to prepare catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films via a solution blending method in neutral aqueous solution for active food packaging.
33910732	0	16	theme	active	144:149	arg1	packaging					156:164	active food packaging	144:164	active food packaging	144:164	Improved mechanical, antibacterial and UV barrier properties of catechol-functionalized chitosan/polyvinyl alcohol biodegradable composites for active food packaging.
33910732	1	17	theme	aqueous	381:387	arg1	solution					389:396	neutral aqueous solution	373:396	neutral aqueous solution	373:396	Bioinspired from adhesion behaviors of mussels, we first reported a new strategy to prepare catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films via a solution blending method in neutral aqueous solution for active food packaging.
33910732	6	18	theme	appropriate	1025:1035	arg1	C-CS					1047:1050	C-CS	1047:1050	C-CS	1047:1050	The water resistance of C-CS/PVA composite films can not be significantly deteriorated by the appropriate amount of C-CS.
33910732	6	18	theme	appropriate	1025:1035	arg1	amount					1037:1042	the appropriate amount	1021:1042	the appropriate amount of C-CS	1021:1050	The water resistance of C-CS/PVA composite films can not be significantly deteriorated by the appropriate amount of C-CS.
33910732	1	19	theme	mussels	206:212	arg1	behaviors					193:201	adhesion behaviors	184:201	adhesion behaviors of mussels	184:212	Bioinspired from adhesion behaviors of mussels, we first reported a new strategy to prepare catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films via a solution blending method in neutral aqueous solution for active food packaging.
33910732	2	20	theme	composite	499:507	arg1	films					509:513	C-CS/PVA composite films	490:513	C-CS/PVA composite films	490:513	Compared with pure PVA film, the UV transmittance (at 280 nm) of C-CS/PVA composite films decreases by 67.6 % when C-CS content reaches 10 wt%.
33910732	1	21	from	method	363:368	arg1	solution					389:396	neutral aqueous solution	373:396	neutral aqueous solution	373:396	Bioinspired from adhesion behaviors of mussels, we first reported a new strategy to prepare catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films via a solution blending method in neutral aqueous solution for active food packaging.
33910732	2	22	theme	UV	458:459	arg1	transmittance					461:473	the UV transmittance	454:473	the UV transmittance (at 280 nm) of C-CS/PVA composite films	454:513	Compared with pure PVA film, the UV transmittance (at 280 nm) of C-CS/PVA composite films decreases by 67.6 % when C-CS content reaches 10 wt%.
33910732	2	23	theme	films	509:513	arg1	transmittance					461:473	the UV transmittance	454:473	the UV transmittance (at 280 nm) of C-CS/PVA composite films	454:513	Compared with pure PVA film, the UV transmittance (at 280 nm) of C-CS/PVA composite films decreases by 67.6 % when C-CS content reaches 10 wt%.
33910732	0	24	theme	mechanical	9:18	arg1	properties					50:59	mechanical, antibacterial and UV barrier properties	9:59	properties	50:59	Improved mechanical, antibacterial and UV barrier properties of catechol-functionalized chitosan/polyvinyl alcohol biodegradable composites for active food packaging.
33910732	2	25	theme	C-CS/PVA	490:497	arg1	films					509:513	C-CS/PVA composite films	490:513	C-CS/PVA composite films	490:513	Compared with pure PVA film, the UV transmittance (at 280 nm) of C-CS/PVA composite films decreases by 67.6 % when C-CS content reaches 10 wt%.
33910732	4	26	theme	C-CS/PVA	703:710	arg1	films					722:726	C-CS/PVA composite films	703:726	C-CS/PVA composite films	703:726	The maximal tensile strength and elongation at break of C-CS/PVA composite films can reach 45.2 MPa and 153 % respectively, which are 46.3 % and 25.4 % higher than those of pure PVA film.
33910732	1	27	theme	alcohol	309:315	arg1	films					333:337	catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films	259:337	catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films	259:337	Bioinspired from adhesion behaviors of mussels, we first reported a new strategy to prepare catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films via a solution blending method in neutral aqueous solution for active food packaging.
33910732	5	28	theme	PVA	866:868	arg1	matrix					870:875	PVA matrix	866:875	PVA matrix	866:875	The incorporation of C-CS into PVA matrix increases the antibacterial properties significantly.
33910732	2	29	from	nm	483:484	arg1	transmittance					461:473	the UV transmittance	454:473	the UV transmittance (at 280 nm) of C-CS/PVA composite films	454:513	Compared with pure PVA film, the UV transmittance (at 280 nm) of C-CS/PVA composite films decreases by 67.6 % when C-CS content reaches 10 wt%.
33910732	0	30	theme	antibacterial	21:33	arg1	properties					50:59	mechanical, antibacterial and UV barrier properties	9:59	properties	50:59	Improved mechanical, antibacterial and UV barrier properties of catechol-functionalized chitosan/polyvinyl alcohol biodegradable composites for active food packaging.
33910732	2	31	theme	C-CS	540:543	arg1	content					545:551	C-CS content	540:551	C-CS content	540:551	Compared with pure PVA film, the UV transmittance (at 280 nm) of C-CS/PVA composite films decreases by 67.6 % when C-CS content reaches 10 wt%.
33910732	7	32	theme	UV	1189:1190	arg1	mechanical					1159:1168	mechanical	1159:1168	mechanical	1159:1168	Therefore, C-CS/PVA composite films show great potential in the field of active packaging due to its good mechanical, antibacterial and UV barrier properties.
33910732	7	32	theme	UV	1189:1190	arg1	properties					1200:1209	UV barrier properties	1189:1209	UV barrier properties	1189:1209	Therefore, C-CS/PVA composite films show great potential in the field of active packaging due to its good mechanical, antibacterial and UV barrier properties.
33910732	4	33	theme	films	722:726	arg1	elongation					680:689	elongation	680:689	elongation	680:689	The maximal tensile strength and elongation at break of C-CS/PVA composite films can reach 45.2 MPa and 153 % respectively, which are 46.3 % and 25.4 % higher than those of pure PVA film.
33910732	4	33	theme	films	722:726	arg1	strength					667:674	maximal tensile strength	651:674	maximal tensile strength	651:674	The maximal tensile strength and elongation at break of C-CS/PVA composite films can reach 45.2 MPa and 153 % respectively, which are 46.3 % and 25.4 % higher than those of pure PVA film.
33910732	2	34	theme	PVA	444:446	arg1	film					448:451	pure PVA film	439:451	pure PVA film	439:451	Compared with pure PVA film, the UV transmittance (at 280 nm) of C-CS/PVA composite films decreases by 67.6 % when C-CS content reaches 10 wt%.
33910732	1	35	theme	PVA	318:320	arg1	films					333:337	catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films	259:337	catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films	259:337	Bioinspired from adhesion behaviors of mussels, we first reported a new strategy to prepare catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films via a solution blending method in neutral aqueous solution for active food packaging.
33910732	0	36	theme	food	151:154	arg1	packaging					156:164	active food packaging	144:164	active food packaging	144:164	Improved mechanical, antibacterial and UV barrier properties of catechol-functionalized chitosan/polyvinyl alcohol biodegradable composites for active food packaging.
33910732	1	37	theme	active	402:407	arg1	packaging					414:422	active food packaging	402:422	active food packaging	402:422	Bioinspired from adhesion behaviors of mussels, we first reported a new strategy to prepare catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films via a solution blending method in neutral aqueous solution for active food packaging.
33910732	4	38	theme	composite	712:720	arg1	films					722:726	C-CS/PVA composite films	703:726	C-CS/PVA composite films	703:726	The maximal tensile strength and elongation at break of C-CS/PVA composite films can reach 45.2 MPa and 153 % respectively, which are 46.3 % and 25.4 % higher than those of pure PVA film.
33910732	2	39	theme	pure	439:442	arg1	film					448:451	pure PVA film	439:451	pure PVA film	439:451	Compared with pure PVA film, the UV transmittance (at 280 nm) of C-CS/PVA composite films decreases by 67.6 % when C-CS content reaches 10 wt%.
33910732	0	40	theme	UV	39:40	arg1	properties					50:59	mechanical, antibacterial and UV barrier properties	9:59	properties	50:59	Improved mechanical, antibacterial and UV barrier properties of catechol-functionalized chitosan/polyvinyl alcohol biodegradable composites for active food packaging.
33910732	1	41	theme	food	409:412	arg1	packaging					414:422	active food packaging	402:422	active food packaging	402:422	Bioinspired from adhesion behaviors of mussels, we first reported a new strategy to prepare catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films via a solution blending method in neutral aqueous solution for active food packaging.
33910732	1	42	theme	new	235:237	arg1	strategy					239:246	a new strategy	233:246	a new strategy to prepare catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films via a solution blending method in neutral aqueous solution for active food packaging	233:422	Bioinspired from adhesion behaviors of mussels, we first reported a new strategy to prepare catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films via a solution blending method in neutral aqueous solution for active food packaging.
33910732	3	43	from	transparent	613:623	arg1	range					640:644	the visible range	628:644	the visible range	628:644	Still, all the C-CS/PVA composite films are transparent in the visible range.
33910732	1	44	theme	composite	323:331	arg1	films					333:337	catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films	259:337	catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films	259:337	Bioinspired from adhesion behaviors of mussels, we first reported a new strategy to prepare catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films via a solution blending method in neutral aqueous solution for active food packaging.
33910732	6	45	theme	water	935:939	arg1	resistance					941:950	The water resistance	931:950	The water resistance of C-CS/PVA composite films	931:978	The water resistance of C-CS/PVA composite films can not be significantly deteriorated by the appropriate amount of C-CS.
33910732	6	46	theme	C-CS/PVA	955:962	arg1	films					974:978	C-CS/PVA composite films	955:978	C-CS/PVA composite films	955:978	The water resistance of C-CS/PVA composite films can not be significantly deteriorated by the appropriate amount of C-CS.
33910732	7	47	theme	packaging	1133:1141	arg1	field					1117:1121	the field	1113:1121	the field of active packaging due to its good mechanical, antibacterial and UV barrier properties	1113:1209	Therefore, C-CS/PVA composite films show great potential in the field of active packaging due to its good mechanical, antibacterial and UV barrier properties.
33910732	7	48	theme	due	1143:1145	arg1	packaging					1133:1141	active packaging	1126:1141	active packaging due to its good mechanical, antibacterial and UV barrier properties	1126:1209	Therefore, C-CS/PVA composite films show great potential in the field of active packaging due to its good mechanical, antibacterial and UV barrier properties.
33910732	2	49	theme	wt	564:565	arg1	%					566:566	10 wt%	561:566	10 wt%	561:566	Compared with pure PVA film, the UV transmittance (at 280 nm) of C-CS/PVA composite films decreases by 67.6 % when C-CS content reaches 10 wt%.
33910732	0	50	theme	barrier	42:48	arg1	properties					50:59	mechanical, antibacterial and UV barrier properties	9:59	properties	50:59	Improved mechanical, antibacterial and UV barrier properties of catechol-functionalized chitosan/polyvinyl alcohol biodegradable composites for active food packaging.
33910732	4	51	theme	pure	820:823	arg1	film					829:832	pure PVA film	820:832	pure PVA film	820:832	The maximal tensile strength and elongation at break of C-CS/PVA composite films can reach 45.2 MPa and 153 % respectively, which are 46.3 % and 25.4 % higher than those of pure PVA film.
33910732	4	52	from	break	694:698	arg1	elongation					680:689	elongation	680:689	elongation	680:689	The maximal tensile strength and elongation at break of C-CS/PVA composite films can reach 45.2 MPa and 153 % respectively, which are 46.3 % and 25.4 % higher than those of pure PVA film.
33910732	4	52	from	break	694:698	arg1	strength					667:674	maximal tensile strength	651:674	maximal tensile strength	651:674	The maximal tensile strength and elongation at break of C-CS/PVA composite films can reach 45.2 MPa and 153 % respectively, which are 46.3 % and 25.4 % higher than those of pure PVA film.
33910732	7	53	theme	barrier	1192:1198	arg1	mechanical					1159:1168	mechanical	1159:1168	mechanical	1159:1168	Therefore, C-CS/PVA composite films show great potential in the field of active packaging due to its good mechanical, antibacterial and UV barrier properties.
33910732	7	53	theme	barrier	1192:1198	arg1	properties					1200:1209	UV barrier properties	1189:1209	UV barrier properties	1189:1209	Therefore, C-CS/PVA composite films show great potential in the field of active packaging due to its good mechanical, antibacterial and UV barrier properties.
33910732	3	54	theme	C-CS/PVA	584:591	arg1	transparent					613:623	transparent	613:623	transparent	613:623	Still, all the C-CS/PVA composite films are transparent in the visible range.
33910732	3	54	theme	C-CS/PVA	584:591	arg1	films					603:607	all the C-CS/PVA composite films	576:607	all the C-CS/PVA composite films	576:607	Still, all the C-CS/PVA composite films are transparent in the visible range.
33910732	6	55	theme	films	974:978	arg1	resistance					941:950	The water resistance	931:950	The water resistance of C-CS/PVA composite films	931:978	The water resistance of C-CS/PVA composite films can not be significantly deteriorated by the appropriate amount of C-CS.
33910732	3	56	theme	composite	593:601	arg1	transparent					613:623	transparent	613:623	transparent	613:623	Still, all the C-CS/PVA composite films are transparent in the visible range.
33910732	3	56	theme	composite	593:601	arg1	films					603:607	all the C-CS/PVA composite films	576:607	all the C-CS/PVA composite films	576:607	Still, all the C-CS/PVA composite films are transparent in the visible range.
33910732	5	57	theme	C-CS	856:859	arg1	incorporation					839:851	The incorporation	835:851	The incorporation of C-CS into PVA matrix	835:875	The incorporation of C-CS into PVA matrix increases the antibacterial properties significantly.
33910732	6	58	theme	composite	964:972	arg1	films					974:978	C-CS/PVA composite films	955:978	C-CS/PVA composite films	955:978	The water resistance of C-CS/PVA composite films can not be significantly deteriorated by the appropriate amount of C-CS.
33910732	0	59	theme	catechol-functionalized	64:86	arg1	composites					129:138	catechol-functionalized chitosan/polyvinyl alcohol biodegradable composites	64:138	catechol-functionalized chitosan/polyvinyl alcohol biodegradable composites for active food packaging	64:164	Improved mechanical, antibacterial and UV barrier properties of catechol-functionalized chitosan/polyvinyl alcohol biodegradable composites for active food packaging.
33910732	3	60	theme	visible	632:638	arg1	range					640:644	the visible range	628:644	the visible range	628:644	Still, all the C-CS/PVA composite films are transparent in the visible range.
33910732	4	61	theme	maximal	651:657	arg1	strength					667:674	maximal tensile strength	651:674	maximal tensile strength	651:674	The maximal tensile strength and elongation at break of C-CS/PVA composite films can reach 45.2 MPa and 153 % respectively, which are 46.3 % and 25.4 % higher than those of pure PVA film.
33910732	1	62	theme	catechol-functionalized	259:281	arg1	films					333:337	catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films	259:337	catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films	259:337	Bioinspired from adhesion behaviors of mussels, we first reported a new strategy to prepare catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films via a solution blending method in neutral aqueous solution for active food packaging.
33910732	7	63	theme	active	1126:1131	arg1	packaging					1133:1141	active packaging	1126:1141	active packaging due to its good mechanical, antibacterial and UV barrier properties	1126:1209	Therefore, C-CS/PVA composite films show great potential in the field of active packaging due to its good mechanical, antibacterial and UV barrier properties.
33910732	1	64	theme	adhesion	184:191	arg1	behaviors					193:201	adhesion behaviors	184:201	adhesion behaviors of mussels	184:212	Bioinspired from adhesion behaviors of mussels, we first reported a new strategy to prepare catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films via a solution blending method in neutral aqueous solution for active food packaging.
33910732	1	65	theme	chitosan	283:290	arg1	films					333:337	catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films	259:337	catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films	259:337	Bioinspired from adhesion behaviors of mussels, we first reported a new strategy to prepare catechol-functionalized chitosan (C-CS)/polyvinyl alcohol (PVA) composite films via a solution blending method in neutral aqueous solution for active food packaging.
34388876	0	0	theme	apple	84:88	arg1	clarification					96:108	apple juice clarification	84:108	apple juice clarification	84:108	Removal of difenoconazole and nitenpyram by composite calcium alginate beads during apple juice clarification.
34388876	9	1	theme	main	1316:1319	arg1	factors					1321:1327	the main factors	1312:1327	the main factors for nitenpyram	1312:1342	Hydrophobic and π-π conjugation interactions played a dominant role for the adsorption of difenoconazole, while hydrogen bonding and electrostatic interactions were the main factors for nitenpyram.
34388876	9	1	theme	main	1316:1319	arg1	bonding					1268:1274	hydrogen bonding	1259:1274	hydrogen bonding	1259:1274	Hydrophobic and π-π conjugation interactions played a dominant role for the adsorption of difenoconazole, while hydrogen bonding and electrostatic interactions were the main factors for nitenpyram.
34388876	9	1	theme	main	1316:1319	arg1	interactions					1294:1305	electrostatic interactions	1280:1305	electrostatic interactions	1280:1305	Hydrophobic and π-π conjugation interactions played a dominant role for the adsorption of difenoconazole, while hydrogen bonding and electrostatic interactions were the main factors for nitenpyram.
34388876	5	2	theme	kinetic	914:920	arg1	model					922:926	the pseudo-second-order kinetic model	890:926	the pseudo-second-order kinetic model (R2 = 0.9993, 0.9999)	890:948	The adsorption data were fitted to the Freundlich isotherm model (R2 = 0.9604, 0.9625) and the pseudo-second-order kinetic model (R2 = 0.9993, 0.9999).
34388876	2	3	with	beads	338:342	arg1	risks					370:374	low potential health risks	349:374	low potential health risks for all of the main materials	349:404	The composite beads with low potential health risks for all of the main materials were natural, green and biocompatible.
34388876	7	4	theme	several	1067:1073	arg1	factors					1075:1081	several factors	1067:1081	several factors	1067:1081	Moreover, the rate was controlled by several factors.
34388876	6	5	theme	beads	1005:1009	arg1	behavior					993:1000	the adsorption behavior	978:1000	the adsorption behavior of beads	978:1009	The results indicated that the adsorption behavior of beads was heterogeneous.
34388876	6	5	theme	beads	1005:1009	arg1	heterogeneous					1015:1027	heterogeneous	1015:1027	heterogeneous	1015:1027	The results indicated that the adsorption behavior of beads was heterogeneous.
34388876	9	6	theme	dominant	1201:1208	arg1	role					1210:1213	a dominant role	1199:1213	a dominant role for the adsorption of difenoconazole	1199:1250	Hydrophobic and π-π conjugation interactions played a dominant role for the adsorption of difenoconazole, while hydrogen bonding and electrostatic interactions were the main factors for nitenpyram.
34388876	5	7	theme	pseudo-second-order	894:912	arg1	model					922:926	the pseudo-second-order kinetic model	890:926	the pseudo-second-order kinetic model (R2 = 0.9993, 0.9999)	890:948	The adsorption data were fitted to the Freundlich isotherm model (R2 = 0.9604, 0.9625) and the pseudo-second-order kinetic model (R2 = 0.9993, 0.9999).
34388876	4	8	theme	adsorbent	767:775	arg1	levels					695:700	the pesticide residue levels	673:700	the pesticide residue levels	673:700	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	4	8	theme	adsorbent	767:775	arg1	dose					777:780	adsorbent dose	767:780	adsorbent dose	767:780	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	0	9	theme	juice	90:94	arg1	clarification					96:108	apple juice clarification	84:108	apple juice clarification	84:108	Removal of difenoconazole and nitenpyram by composite calcium alginate beads during apple juice clarification.
34388876	3	10	theme	additional	571:580	arg1	steps					582:586	additional steps	571:586	additional steps	571:586	Since g-Alg/CS/PDA can both clarify and adsorb, pesticide residues could be removed during the clarification of juice without additional steps.
34388876	2	11	theme	potential	353:361	arg1	risks					370:374	low potential health risks	349:374	low potential health risks for all of the main materials	349:404	The composite beads with low potential health risks for all of the main materials were natural, green and biocompatible.
34388876	3	12	theme	juice	557:561	arg1	clarification					540:552	the clarification	536:552	the clarification of juice without additional steps	536:586	Since g-Alg/CS/PDA can both clarify and adsorb, pesticide residues could be removed during the clarification of juice without additional steps.
34388876	4	13	theme	residue	687:693	arg1	strength					730:737	ionic strength	724:737	ionic strength	724:737	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	4	13	theme	residue	687:693	arg1	time					714:717	adsorption time	703:717	adsorption time	703:717	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	4	13	theme	residue	687:693	arg1	pH					720:721	pH	720:721	pH	720:721	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	4	13	theme	residue	687:693	arg1	concentration					749:761	fructose concentration	740:761	fructose concentration	740:761	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	4	13	theme	residue	687:693	arg1	levels					695:700	the pesticide residue levels	673:700	the pesticide residue levels	673:700	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	4	13	theme	residue	687:693	arg1	dose					777:780	adsorbent dose	767:780	adsorbent dose	767:780	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	6	14	theme	adsorption	982:991	arg1	behavior					993:1000	the adsorption behavior	978:1000	the adsorption behavior of beads	978:1009	The results indicated that the adsorption behavior of beads was heterogeneous.
34388876	6	14	theme	adsorption	982:991	arg1	heterogeneous					1015:1027	heterogeneous	1015:1027	heterogeneous	1015:1027	The results indicated that the adsorption behavior of beads was heterogeneous.
34388876	2	15	theme	low	349:351	arg1	risks					370:374	low potential health risks	349:374	low potential health risks for all of the main materials	349:404	The composite beads with low potential health risks for all of the main materials were natural, green and biocompatible.
34388876	4	16	theme	adsorption	703:712	arg1	levels					695:700	the pesticide residue levels	673:700	the pesticide residue levels	673:700	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	4	16	theme	adsorption	703:712	arg1	time					714:717	adsorption time	703:717	adsorption time	703:717	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	1	17	theme	apple	311:315	arg1	juice					317:321	apple juice	311:321	apple juice	311:321	A novel genipin crosslinked calcium alginate/chitosan/polydopamine composite beads (g-Alg/CS/PDA) was synthesized for the removal of residual difenoconazole and nitenpyram during the clarification of apple juice.
34388876	5	18	dep	model	922:926	arg1	R2 = 0.9993					929:939	R2 = 0.9993	929:939	R2 = 0.9993	929:939	The adsorption data were fitted to the Freundlich isotherm model (R2 = 0.9604, 0.9625) and the pseudo-second-order kinetic model (R2 = 0.9993, 0.9999).
34388876	1	19	theme	juice	317:321	arg1	clarification					294:306	the clarification	290:306	the clarification of apple juice	290:321	A novel genipin crosslinked calcium alginate/chitosan/polydopamine composite beads (g-Alg/CS/PDA) was synthesized for the removal of residual difenoconazole and nitenpyram during the clarification of apple juice.
34388876	8	20	theme	adsorption	1088:1097	arg1	mechanism					1099:1107	The adsorption mechanism	1084:1107	The adsorption mechanism of two pesticides	1084:1125	The adsorption mechanism of two pesticides was also discussed.
34388876	9	21	theme	difenoconazole	1237:1250	arg1	adsorption					1223:1232	the adsorption	1219:1232	the adsorption of difenoconazole	1219:1250	Hydrophobic and π-π conjugation interactions played a dominant role for the adsorption of difenoconazole, while hydrogen bonding and electrostatic interactions were the main factors for nitenpyram.
34388876	5	22	theme	isotherm	849:856	arg1	model					858:862	the Freundlich isotherm model	834:862	the Freundlich isotherm model (R2 = 0.9604, 0.9625)	834:884	The adsorption data were fitted to the Freundlich isotherm model (R2 = 0.9604, 0.9625) and the pseudo-second-order kinetic model (R2 = 0.9993, 0.9999).
34388876	0	23	theme	difenoconazole	11:24	arg1	Removal					0:6	Removal	0:6	Removal of difenoconazole and nitenpyram by composite calcium alginate beads during apple juice clarification.	0:109	Removal of difenoconazole and nitenpyram by composite calcium alginate beads during apple juice clarification.
34388876	4	24	theme	g-Alg/CS/PDA	593:604	arg1	beads					606:610	The g-Alg/CS/PDA beads	589:610	The g-Alg/CS/PDA beads	589:610	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	2	25	theme	composite	328:336	arg1	natural					411:417	natural	411:417	natural	411:417	The composite beads with low potential health risks for all of the main materials were natural, green and biocompatible.
34388876	2	25	theme	composite	328:336	arg1	beads					338:342	The composite beads	324:342	The composite beads with low potential health risks for all of the main materials	324:404	The composite beads with low potential health risks for all of the main materials were natural, green and biocompatible.
34388876	1	26	theme	alginate/chitosan/polydopamine	147:176	arg1	g-Alg/CS/PDA					195:206	g-Alg/CS/PDA	195:206	g-Alg/CS/PDA	195:206	A novel genipin crosslinked calcium alginate/chitosan/polydopamine composite beads (g-Alg/CS/PDA) was synthesized for the removal of residual difenoconazole and nitenpyram during the clarification of apple juice.
34388876	1	26	theme	alginate/chitosan/polydopamine	147:176	arg1	beads					188:192	calcium alginate/chitosan/polydopamine composite beads	139:192	calcium alginate/chitosan/polydopamine composite beads (g-Alg/CS/PDA)	139:207	A novel genipin crosslinked calcium alginate/chitosan/polydopamine composite beads (g-Alg/CS/PDA) was synthesized for the removal of residual difenoconazole and nitenpyram during the clarification of apple juice.
34388876	4	27	theme	ionic	724:728	arg1	strength					730:737	ionic strength	724:737	ionic strength	724:737	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	4	27	theme	ionic	724:728	arg1	levels					695:700	the pesticide residue levels	673:700	the pesticide residue levels	673:700	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	4	28	theme	pesticide	677:685	arg1	strength					730:737	ionic strength	724:737	ionic strength	724:737	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	4	28	theme	pesticide	677:685	arg1	time					714:717	adsorption time	703:717	adsorption time	703:717	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	4	28	theme	pesticide	677:685	arg1	pH					720:721	pH	720:721	pH	720:721	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	4	28	theme	pesticide	677:685	arg1	concentration					749:761	fructose concentration	740:761	fructose concentration	740:761	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	4	28	theme	pesticide	677:685	arg1	levels					695:700	the pesticide residue levels	673:700	the pesticide residue levels	673:700	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	4	28	theme	pesticide	677:685	arg1	dose					777:780	adsorbent dose	767:780	adsorbent dose	767:780	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	0	29	theme	nitenpyram	30:39	arg1	Removal					0:6	Removal	0:6	Removal of difenoconazole and nitenpyram by composite calcium alginate beads during apple juice clarification.	0:109	Removal of difenoconazole and nitenpyram by composite calcium alginate beads during apple juice clarification.
34388876	5	30	theme	adsorption	803:812	arg1	data					814:817	The adsorption data	799:817	The adsorption data	799:817	The adsorption data were fitted to the Freundlich isotherm model (R2 = 0.9604, 0.9625) and the pseudo-second-order kinetic model (R2 = 0.9993, 0.9999).
34388876	9	31	theme	hydrogen	1259:1266	arg1	factors					1321:1327	the main factors	1312:1327	the main factors for nitenpyram	1312:1342	Hydrophobic and π-π conjugation interactions played a dominant role for the adsorption of difenoconazole, while hydrogen bonding and electrostatic interactions were the main factors for nitenpyram.
34388876	9	31	theme	hydrogen	1259:1266	arg1	bonding					1268:1274	hydrogen bonding	1259:1274	hydrogen bonding	1259:1274	Hydrophobic and π-π conjugation interactions played a dominant role for the adsorption of difenoconazole, while hydrogen bonding and electrostatic interactions were the main factors for nitenpyram.
34388876	9	31	theme	hydrogen	1259:1266	arg1	interactions					1294:1305	electrostatic interactions	1280:1305	electrostatic interactions	1280:1305	Hydrophobic and π-π conjugation interactions played a dominant role for the adsorption of difenoconazole, while hydrogen bonding and electrostatic interactions were the main factors for nitenpyram.
34388876	0	32	theme	calcium	54:60	arg1	beads					71:75	composite calcium alginate beads	44:75	composite calcium alginate beads during apple juice clarification	44:108	Removal of difenoconazole and nitenpyram by composite calcium alginate beads during apple juice clarification.
34388876	8	33	theme	pesticides	1116:1125	arg1	mechanism					1099:1107	The adsorption mechanism	1084:1107	The adsorption mechanism of two pesticides	1084:1125	The adsorption mechanism of two pesticides was also discussed.
34388876	1	34	theme	novel	113:117	arg1	genipin					119:125	A novel genipin	111:125	A novel genipin crosslinked calcium alginate/chitosan/polydopamine composite beads (g-Alg/CS/PDA)	111:207	A novel genipin crosslinked calcium alginate/chitosan/polydopamine composite beads (g-Alg/CS/PDA) was synthesized for the removal of residual difenoconazole and nitenpyram during the clarification of apple juice.
34388876	0	35	theme	composite	44:52	arg1	beads					71:75	composite calcium alginate beads	44:75	composite calcium alginate beads during apple juice clarification	44:108	Removal of difenoconazole and nitenpyram by composite calcium alginate beads during apple juice clarification.
34388876	3	36	theme	pesticide	493:501	arg1	residues					503:510	pesticide residues	493:510	pesticide residues	493:510	Since g-Alg/CS/PDA can both clarify and adsorb, pesticide residues could be removed during the clarification of juice without additional steps.
34388876	9	37	theme	Hydrophobic	1147:1157	arg1	interactions					1179:1190	Hydrophobic and π-π conjugation interactions	1147:1190	Hydrophobic and π-π conjugation interactions	1147:1190	Hydrophobic and π-π conjugation interactions played a dominant role for the adsorption of difenoconazole, while hydrogen bonding and electrostatic interactions were the main factors for nitenpyram.
34388876	4	38	theme	fructose	740:747	arg1	levels					695:700	the pesticide residue levels	673:700	the pesticide residue levels	673:700	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	4	38	theme	fructose	740:747	arg1	concentration					749:761	fructose concentration	740:761	fructose concentration	740:761	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	5	39	dep	model	858:862	arg1	R2 = 0.9604					865:875	R2 = 0.9604	865:875	R2 = 0.9604	865:875	The adsorption data were fitted to the Freundlich isotherm model (R2 = 0.9604, 0.9625) and the pseudo-second-order kinetic model (R2 = 0.9993, 0.9999).
34388876	9	40	theme	electrostatic	1280:1292	arg1	factors					1321:1327	the main factors	1312:1327	the main factors for nitenpyram	1312:1342	Hydrophobic and π-π conjugation interactions played a dominant role for the adsorption of difenoconazole, while hydrogen bonding and electrostatic interactions were the main factors for nitenpyram.
34388876	9	40	theme	electrostatic	1280:1292	arg1	bonding					1268:1274	hydrogen bonding	1259:1274	hydrogen bonding	1259:1274	Hydrophobic and π-π conjugation interactions played a dominant role for the adsorption of difenoconazole, while hydrogen bonding and electrostatic interactions were the main factors for nitenpyram.
34388876	9	40	theme	electrostatic	1280:1292	arg1	interactions					1294:1305	electrostatic interactions	1280:1305	electrostatic interactions	1280:1305	Hydrophobic and π-π conjugation interactions played a dominant role for the adsorption of difenoconazole, while hydrogen bonding and electrostatic interactions were the main factors for nitenpyram.
34388876	2	41	theme	health	363:368	arg1	risks					370:374	low potential health risks	349:374	low potential health risks for all of the main materials	349:404	The composite beads with low potential health risks for all of the main materials were natural, green and biocompatible.
34388876	1	42	theme	residual	244:251	arg1	difenoconazole					253:266	residual difenoconazole	244:266	residual difenoconazole	244:266	A novel genipin crosslinked calcium alginate/chitosan/polydopamine composite beads (g-Alg/CS/PDA) was synthesized for the removal of residual difenoconazole and nitenpyram during the clarification of apple juice.
34388876	5	43	theme	Freundlich	838:847	arg1	model					858:862	the Freundlich isotherm model	834:862	the Freundlich isotherm model (R2 = 0.9604, 0.9625)	834:884	The adsorption data were fitted to the Freundlich isotherm model (R2 = 0.9604, 0.9625) and the pseudo-second-order kinetic model (R2 = 0.9993, 0.9999).
34388876	9	44	theme	π-π	1163:1165	arg1	interactions					1179:1190	Hydrophobic and π-π conjugation interactions	1147:1190	Hydrophobic and π-π conjugation interactions	1147:1190	Hydrophobic and π-π conjugation interactions played a dominant role for the adsorption of difenoconazole, while hydrogen bonding and electrostatic interactions were the main factors for nitenpyram.
34388876	1	45	theme	calcium	139:145	arg1	g-Alg/CS/PDA					195:206	g-Alg/CS/PDA	195:206	g-Alg/CS/PDA	195:206	A novel genipin crosslinked calcium alginate/chitosan/polydopamine composite beads (g-Alg/CS/PDA) was synthesized for the removal of residual difenoconazole and nitenpyram during the clarification of apple juice.
34388876	1	45	theme	calcium	139:145	arg1	beads					188:192	calcium alginate/chitosan/polydopamine composite beads	139:192	calcium alginate/chitosan/polydopamine composite beads (g-Alg/CS/PDA)	139:207	A novel genipin crosslinked calcium alginate/chitosan/polydopamine composite beads (g-Alg/CS/PDA) was synthesized for the removal of residual difenoconazole and nitenpyram during the clarification of apple juice.
34388876	1	46	theme	difenoconazole	253:266	arg1	removal					233:239	the removal	229:239	the removal of residual difenoconazole and nitenpyram	229:281	A novel genipin crosslinked calcium alginate/chitosan/polydopamine composite beads (g-Alg/CS/PDA) was synthesized for the removal of residual difenoconazole and nitenpyram during the clarification of apple juice.
34388876	9	47	theme	conjugation	1167:1177	arg1	interactions					1179:1190	Hydrophobic and π-π conjugation interactions	1147:1190	Hydrophobic and π-π conjugation interactions	1147:1190	Hydrophobic and π-π conjugation interactions played a dominant role for the adsorption of difenoconazole, while hydrogen bonding and electrostatic interactions were the main factors for nitenpyram.
34388876	2	48	theme	main	391:394	arg1	materials					396:404	the main materials	387:404	the main materials	387:404	The composite beads with low potential health risks for all of the main materials were natural, green and biocompatible.
34388876	0	49	theme	alginate	62:69	arg1	beads					71:75	composite calcium alginate beads	44:75	composite calcium alginate beads during apple juice clarification	44:108	Removal of difenoconazole and nitenpyram by composite calcium alginate beads during apple juice clarification.
34388876	4	50	theme	adsorption	640:649	arg1	levels					695:700	the pesticide residue levels	673:700	the pesticide residue levels	673:700	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	4	50	theme	adsorption	640:649	arg1	parameters					651:660	the adsorption parameters	636:660	the adsorption parameters	636:660	The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized.
34388876	1	51	theme	composite	178:186	arg1	g-Alg/CS/PDA					195:206	g-Alg/CS/PDA	195:206	g-Alg/CS/PDA	195:206	A novel genipin crosslinked calcium alginate/chitosan/polydopamine composite beads (g-Alg/CS/PDA) was synthesized for the removal of residual difenoconazole and nitenpyram during the clarification of apple juice.
34388876	1	51	theme	composite	178:186	arg1	beads					188:192	calcium alginate/chitosan/polydopamine composite beads	139:192	calcium alginate/chitosan/polydopamine composite beads (g-Alg/CS/PDA)	139:207	A novel genipin crosslinked calcium alginate/chitosan/polydopamine composite beads (g-Alg/CS/PDA) was synthesized for the removal of residual difenoconazole and nitenpyram during the clarification of apple juice.
34388876	1	52	theme	nitenpyram	272:281	arg1	removal					233:239	the removal	229:239	the removal of residual difenoconazole and nitenpyram	229:281	A novel genipin crosslinked calcium alginate/chitosan/polydopamine composite beads (g-Alg/CS/PDA) was synthesized for the removal of residual difenoconazole and nitenpyram during the clarification of apple juice.
34224554	3	0	theme	particle	498:505	arg1	size					507:510	reduced particle size	490:510	reduced particle size (WB RPS)	490:519	OBJECTIVES We investigated the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health in normal-weight (NW) and obese (OB) participants compared with placebo (PL).
34224554	3	0	theme	particle	498:505	arg1	RPS					516:518	WB RPS	513:518	WB RPS	513:518	OBJECTIVES We investigated the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health in normal-weight (NW) and obese (OB) participants compared with placebo (PL).
34224554	1	1	theme	reduced	243:249	arg1	risk					251:254	a reduced risk	241:254	a reduced risk of metabolic disorders	241:277	BACKGROUND Wheat bran (WB) has been associated with improved gastrointestinal health and a reduced risk of metabolic disorders.
34224554	3	2	from	impact	472:477	arg1	fermentation					532:543	colonic fermentation	524:543	colonic fermentation	524:543	OBJECTIVES We investigated the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health in normal-weight (NW) and obese (OB) participants compared with placebo (PL).
34224554	3	2	from	impact	472:477	arg1	health					554:559	host health	549:559	host health	549:559	OBJECTIVES We investigated the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health in normal-weight (NW) and obese (OB) participants compared with placebo (PL).
34224554	8	3	from	concentrations	1371:1384	arg1	participants					1392:1403	OB participants	1389:1403	OB participants	1389:1403	RESULTS WB RPS increased fasting serum acetate (P < 0.05) and total SCFA (P < 0.05) concentrations in OB participants.
34224554	3	4	theme	host	549:552	arg1	health					554:559	host health	549:559	host health	549:559	OBJECTIVES We investigated the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health in normal-weight (NW) and obese (OB) participants compared with placebo (PL).
34224554	11	5	dep	affected	1779:1786	arg1	either					1816:1821	either	1816:1821	either	1816:1821	Health parameters were not affected by the WB RPS intervention, either in NW or in OB participants.
34224554	11	5	dep	affected	1779:1786	arg1	participants					1838:1849	OB participants	1835:1849	OB participants	1835:1849	Health parameters were not affected by the WB RPS intervention, either in NW or in OB participants.
34224554	6	6	theme	serum	1008:1012	arg1	weight					924:929	fecal dry weight	914:929	fecal dry weight (%)	914:933	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	6	theme	serum	1008:1012	arg1	permeability					990:1001	intestinal permeability	979:1001	intestinal permeability	979:1001	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	6	theme	serum	1008:1012	arg1	transit					951:957	transit	951:957	transit	951:957	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	6	theme	serum	1008:1012	arg1	output					906:911	Fecal output	900:911	Fecal output	900:911	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	6	theme	serum	1008:1012	arg1	consistency					966:976	stool consistency	960:976	stool consistency	960:976	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	6	theme	serum	1008:1012	arg1	parameters					1109:1118	health parameters	1102:1118	health parameters	1102:1118	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	6	theme	serum	1008:1012	arg1	cholesterol					1020:1030	serum total cholesterol	1008:1030	serum total cholesterol	1008:1030	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	6	theme	serum	1008:1012	arg1	excretion					940:948	fat excretion	936:948	fat excretion	936:948	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	9	7	dep	NW	1500:1501	arg1	P < 0.01					1548:1555	P < 0.01	1548:1555	P < 0.01	1548:1555	Fasting serum propionate concentrations were lower in OB than in NW participants at baseline (NW: 1.57 ± 0.75 µmol/L; OB: 0.89 ± 0.52 µmol/L; P < 0.01), but not after WB RPS (NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant).
34224554	9	7	dep	NW	1500:1501	arg1	OB					1524:1525	OB	1524:1525	NW: 1.57 ± 0.75 µmol/L; OB: 0.89 ± 0.52 µmol/L; P < 0.01	1500:1555	Fasting serum propionate concentrations were lower in OB than in NW participants at baseline (NW: 1.57 ± 0.75 µmol/L; OB: 0.89 ± 0.52 µmol/L; P < 0.01), but not after WB RPS (NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant).
34224554	9	7	dep	NW	1500:1501	arg1	µmol/L					1540:1545	0.89 ± 0.52 µmol/L	1528:1545	NW: 1.57 ± 0.75 µmol/L; OB: 0.89 ± 0.52 µmol/L; P < 0.01	1500:1555	Fasting serum propionate concentrations were lower in OB than in NW participants at baseline (NW: 1.57 ± 0.75 µmol/L; OB: 0.89 ± 0.52 µmol/L; P < 0.01), but not after WB RPS (NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant).
34224554	9	7	dep	NW	1500:1501	arg1	µmol/L					1516:1521	1.57 ± 0.75 µmol/L	1504:1521	NW: 1.57 ± 0.75 µmol/L; OB: 0.89 ± 0.52 µmol/L; P < 0.01	1500:1555	Fasting serum propionate concentrations were lower in OB than in NW participants at baseline (NW: 1.57 ± 0.75 µmol/L; OB: 0.89 ± 0.52 µmol/L; P < 0.01), but not after WB RPS (NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant).
34224554	8	8	theme	acetate	1326:1332	arg1	P < 0.05					1335:1342	fasting serum acetate (P < 0.05) and total SCFA (P < 0.05) concentrations	1312:1384	P < 0.05	1335:1342	RESULTS WB RPS increased fasting serum acetate (P < 0.05) and total SCFA (P < 0.05) concentrations in OB participants.
34224554	9	9	dep	baseline	1490:1497	arg1	NW					1500:1501	NW	1500:1501	NW	1500:1501	Fasting serum propionate concentrations were lower in OB than in NW participants at baseline (NW: 1.57 ± 0.75 µmol/L; OB: 0.89 ± 0.52 µmol/L; P < 0.01), but not after WB RPS (NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant).
34224554	9	10	dep	NW	1581:1582	arg1	P = not					1629:1635	P = not significant	1629:1647	NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant	1581:1647	Fasting serum propionate concentrations were lower in OB than in NW participants at baseline (NW: 1.57 ± 0.75 µmol/L; OB: 0.89 ± 0.52 µmol/L; P < 0.01), but not after WB RPS (NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant).
34224554	9	10	dep	NW	1581:1582	arg1	OB					1605:1606	OB	1605:1606	NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant	1581:1647	Fasting serum propionate concentrations were lower in OB than in NW participants at baseline (NW: 1.57 ± 0.75 µmol/L; OB: 0.89 ± 0.52 µmol/L; P < 0.01), but not after WB RPS (NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant).
34224554	9	10	dep	NW	1581:1582	arg1	µmol/L					1621:1626	1.35 ± 0.63 µmol/L	1609:1626	NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant	1581:1647	Fasting serum propionate concentrations were lower in OB than in NW participants at baseline (NW: 1.57 ± 0.75 µmol/L; OB: 0.89 ± 0.52 µmol/L; P < 0.01), but not after WB RPS (NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant).
34224554	9	10	dep	NW	1581:1582	arg1	µmol/L					1597:1602	1.75 ± 0.77 µmol/L	1585:1602	NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant	1581:1647	Fasting serum propionate concentrations were lower in OB than in NW participants at baseline (NW: 1.57 ± 0.75 µmol/L; OB: 0.89 ± 0.52 µmol/L; P < 0.01), but not after WB RPS (NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant).
34224554	3	11	dep	obese	587:591	arg1	OB					594:595	OB	594:595	OB	594:595	OBJECTIVES We investigated the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health in normal-weight (NW) and obese (OB) participants compared with placebo (PL).
34224554	0	12	from	SCFAs	54:58	arg1	subjects					69:76	obese subjects	63:76	obese subjects	63:76	Wheat bran with reduced particle size increases serum SCFAs in obese subjects without improving health parameters compared with a maltodextrin placebo.
34224554	9	13	theme	WB	1573:1574	arg1	RPS					1576:1578	WB RPS	1573:1578	WB RPS (NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant)	1573:1648	Fasting serum propionate concentrations were lower in OB than in NW participants at baseline (NW: 1.57 ± 0.75 µmol/L; OB: 0.89 ± 0.52 µmol/L; P < 0.01), but not after WB RPS (NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant).
34224554	11	14	theme	WB	1795:1796	arg1	intervention					1802:1813	the WB RPS intervention	1791:1813	the WB RPS intervention	1791:1813	Health parameters were not affected by the WB RPS intervention, either in NW or in OB participants.
34224554	7	15	theme	serum	1237:1241	arg1	13C-SCFA					1243:1250	postprandial cumulative serum 13C-SCFA	1213:1250	postprandial cumulative serum 13C-SCFA	1213:1250	The impact of WB RPS on the fermentation of other carbohydrates was assessed by quantifying postprandial cumulative serum 13C-SCFA after a challenge with 13C-inulin.
34224554	12	16	theme	serum	1889:1893	arg1	concentrations					1900:1913	fasting serum SCFA concentrations	1881:1913	fasting serum SCFA concentrations in OB participants	1881:1932	CONCLUSIONS WB RPS increased fasting serum SCFA concentrations in OB participants.
34224554	3	17	theme	obese	587:591	arg1	participants					598:609	normal-weight (NW) and obese (OB) participants	564:609	normal-weight (NW) and obese (OB) participants compared with placebo (PL)	564:636	OBJECTIVES We investigated the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health in normal-weight (NW) and obese (OB) participants compared with placebo (PL).
34224554	5	18	theme	metabolite	810:819	arg1	SCFAs					797:801	fecal SCFAs	791:801	fecal SCFAs	791:801	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	18	theme	metabolite	810:819	arg1	profiles					821:828	fecal metabolite profiles	804:828	fecal metabolite profiles	804:828	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	18	theme	metabolite	810:819	arg1	parameters					888:897	fermentation parameters	875:897	fermentation parameters	875:897	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	18	theme	metabolite	810:819	arg1	composition					846:856	microbiota composition	835:856	microbiota composition	835:856	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	18	theme	metabolite	810:819	arg1	serum					781:785	fasting serum	773:785	fasting serum	773:785	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	6	19	theme	fecal	914:918	arg1	weight					924:929	fecal dry weight	914:929	fecal dry weight (%)	914:933	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	19	theme	fecal	914:918	arg1	permeability					990:1001	intestinal permeability	979:1001	intestinal permeability	979:1001	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	19	theme	fecal	914:918	arg1	%					932:932	%	932:932	%	932:932	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	19	theme	fecal	914:918	arg1	transit					951:957	transit	951:957	transit	951:957	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	19	theme	fecal	914:918	arg1	output					906:911	Fecal output	900:911	Fecal output	900:911	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	19	theme	fecal	914:918	arg1	consistency					966:976	stool consistency	960:976	stool consistency	960:976	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	19	theme	fecal	914:918	arg1	parameters					1109:1118	health parameters	1102:1118	health parameters	1102:1118	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	19	theme	fecal	914:918	arg1	cholesterol					1020:1030	serum total cholesterol	1008:1030	serum total cholesterol	1008:1030	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	19	theme	fecal	914:918	arg1	excretion					940:948	fat excretion	936:948	fat excretion	936:948	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	8	20	theme	SCFA	1355:1358	arg1	concentrations					1371:1384	fasting serum acetate (P < 0.05) and total SCFA (P < 0.05) concentrations	1312:1384	concentrations	1371:1384	RESULTS WB RPS increased fasting serum acetate (P < 0.05) and total SCFA (P < 0.05) concentrations in OB participants.
34224554	7	21	theme	postprandial	1213:1224	arg1	13C-SCFA					1243:1250	postprandial cumulative serum 13C-SCFA	1213:1250	postprandial cumulative serum 13C-SCFA	1213:1250	The impact of WB RPS on the fermentation of other carbohydrates was assessed by quantifying postprandial cumulative serum 13C-SCFA after a challenge with 13C-inulin.
34224554	8	22	from	P < 0.05	1335:1342	arg1	participants					1392:1403	OB participants	1389:1403	OB participants	1389:1403	RESULTS WB RPS increased fasting serum acetate (P < 0.05) and total SCFA (P < 0.05) concentrations in OB participants.
34224554	8	23	theme	P < 0.05	1361:1368	arg1	concentrations					1371:1384	fasting serum acetate (P < 0.05) and total SCFA (P < 0.05) concentrations	1312:1384	concentrations	1371:1384	RESULTS WB RPS increased fasting serum acetate (P < 0.05) and total SCFA (P < 0.05) concentrations in OB participants.
34224554	12	24	theme	WB	1864:1865	arg1	RPS					1867:1869	CONCLUSIONS WB RPS	1852:1869	CONCLUSIONS WB RPS	1852:1869	CONCLUSIONS WB RPS increased fasting serum SCFA concentrations in OB participants.
34224554	0	25	theme	obese	63:67	arg1	subjects					69:76	obese subjects	63:76	obese subjects	63:76	Wheat bran with reduced particle size increases serum SCFAs in obese subjects without improving health parameters compared with a maltodextrin placebo.
34224554	5	26	theme	fecal	791:795	arg1	composition					846:856	microbiota composition	835:856	microbiota composition	835:856	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	26	theme	fecal	791:795	arg1	profiles					821:828	fecal metabolite profiles	804:828	fecal metabolite profiles	804:828	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	26	theme	fecal	791:795	arg1	parameters					888:897	fermentation parameters	875:897	fermentation parameters	875:897	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	26	theme	fecal	791:795	arg1	SCFAs					797:801	fecal SCFAs	791:801	fecal SCFAs	791:801	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	26	theme	fecal	791:795	arg1	serum					781:785	fasting serum	773:785	fasting serum	773:785	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	11	27	theme	RPS	1798:1800	arg1	intervention					1802:1813	the WB RPS intervention	1791:1813	the WB RPS intervention	1791:1813	Health parameters were not affected by the WB RPS intervention, either in NW or in OB participants.
34224554	9	28	dep	RPS	1576:1578	arg1	NW					1581:1582	NW	1581:1582	NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant	1581:1647	Fasting serum propionate concentrations were lower in OB than in NW participants at baseline (NW: 1.57 ± 0.75 µmol/L; OB: 0.89 ± 0.52 µmol/L; P < 0.01), but not after WB RPS (NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant).
34224554	9	29	theme	propionate	1420:1429	arg1	concentrations					1431:1444	Fasting serum propionate concentrations	1406:1444	Fasting serum propionate concentrations	1406:1444	Fasting serum propionate concentrations were lower in OB than in NW participants at baseline (NW: 1.57 ± 0.75 µmol/L; OB: 0.89 ± 0.52 µmol/L; P < 0.01), but not after WB RPS (NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant).
34224554	8	30	theme	OB	1389:1390	arg1	participants					1392:1403	OB participants	1389:1403	OB participants	1389:1403	RESULTS WB RPS increased fasting serum acetate (P < 0.05) and total SCFA (P < 0.05) concentrations in OB participants.
34224554	0	31	theme	health	96:101	arg1	parameters					103:112	health parameters	96:112	health parameters	96:112	Wheat bran with reduced particle size increases serum SCFAs in obese subjects without improving health parameters compared with a maltodextrin placebo.
34224554	9	32	from	baseline	1490:1497	arg1	participants					1474:1485	NW participants	1471:1485	NW participants at baseline (NW: 1.57 ± 0.75 µmol/L; OB: 0.89 ± 0.52 µmol/L; P < 0.01), but not after WB RPS (NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant)	1471:1648	Fasting serum propionate concentrations were lower in OB than in NW participants at baseline (NW: 1.57 ± 0.75 µmol/L; OB: 0.89 ± 0.52 µmol/L; P < 0.01), but not after WB RPS (NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant).
34224554	0	33	theme	Wheat	0:4	arg1	bran					6:9	Wheat bran	0:9	Wheat bran with reduced particle size	0:36	Wheat bran with reduced particle size increases serum SCFAs in obese subjects without improving health parameters compared with a maltodextrin placebo.
34224554	1	34	theme	metabolic	259:267	arg1	disorders					269:277	metabolic disorders	259:277	metabolic disorders	259:277	BACKGROUND Wheat bran (WB) has been associated with improved gastrointestinal health and a reduced risk of metabolic disorders.
34224554	12	35	from	concentrations	1900:1913	arg1	participants					1921:1932	OB participants	1918:1932	OB participants	1918:1932	CONCLUSIONS WB RPS increased fasting serum SCFA concentrations in OB participants.
34224554	2	36	theme	health	425:430	arg1	effects					432:438	health effects	425:438	health effects	425:438	Reducing the particle size of WB might increase its fermentability and facilitate cross-feeding between the gut bacteria and in this way produce health effects.
34224554	6	37	theme	protein	1062:1068	arg1	concentrations					1070:1083	C-reactive protein concentrations	1051:1083	C-reactive protein concentrations	1051:1083	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	11	38	dep	either	1816:1821	arg1	NW					1826:1827	NW	1826:1827	NW	1826:1827	Health parameters were not affected by the WB RPS intervention, either in NW or in OB participants.
34224554	5	39	theme	microbiota	835:844	arg1	SCFAs					797:801	fecal SCFAs	791:801	fecal SCFAs	791:801	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	39	theme	microbiota	835:844	arg1	profiles					821:828	fecal metabolite profiles	804:828	fecal metabolite profiles	804:828	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	39	theme	microbiota	835:844	arg1	parameters					888:897	fermentation parameters	875:897	fermentation parameters	875:897	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	39	theme	microbiota	835:844	arg1	composition					846:856	microbiota composition	835:856	microbiota composition	835:856	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	39	theme	microbiota	835:844	arg1	serum					781:785	fasting serum	773:785	fasting serum	773:785	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	7	40	theme	carbohydrates	1171:1183	arg1	fermentation					1149:1160	the fermentation	1145:1160	the fermentation of other carbohydrates	1145:1183	The impact of WB RPS on the fermentation of other carbohydrates was assessed by quantifying postprandial cumulative serum 13C-SCFA after a challenge with 13C-inulin.
34224554	9	41	theme	significant	1637:1647	arg1	P = not					1629:1635	P = not significant	1629:1647	NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant	1581:1647	Fasting serum propionate concentrations were lower in OB than in NW participants at baseline (NW: 1.57 ± 0.75 µmol/L; OB: 0.89 ± 0.52 µmol/L; P < 0.01), but not after WB RPS (NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant).
34224554	4	42	theme	PL	719:720	arg1	maltodextrin					723:734	PL (maltodextrin)	719:735	PL (maltodextrin)	719:735	METHODS During 1 mo, 36 NW and 14 OB participants daily consumed 20 g WB RPS or PL (maltodextrin).
34224554	0	43	theme	particle	24:31	arg1	size					33:36	reduced particle size	16:36	reduced particle size	16:36	Wheat bran with reduced particle size increases serum SCFAs in obese subjects without improving health parameters compared with a maltodextrin placebo.
34224554	8	44	theme	WB	1295:1296	arg1	RPS					1298:1300	RESULTS WB RPS	1287:1300	RESULTS WB RPS	1287:1300	RESULTS WB RPS increased fasting serum acetate (P < 0.05) and total SCFA (P < 0.05) concentrations in OB participants.
34224554	3	45	theme	WB	482:483	arg1	impact					472:477	the impact	468:477	the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health	468:559	OBJECTIVES We investigated the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health in normal-weight (NW) and obese (OB) participants compared with placebo (PL).
34224554	6	46	theme	C-reactive	1051:1060	arg1	concentrations					1070:1083	C-reactive protein concentrations	1051:1083	C-reactive protein concentrations	1051:1083	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	1	47	theme	improved	204:211	arg1	health					230:235	improved gastrointestinal health	204:235	improved gastrointestinal health	204:235	BACKGROUND Wheat bran (WB) has been associated with improved gastrointestinal health and a reduced risk of metabolic disorders.
34224554	7	48	theme	RPS	1138:1140	arg1	impact					1125:1130	The impact	1121:1130	The impact of WB RPS on the fermentation of other carbohydrates	1121:1183	The impact of WB RPS on the fermentation of other carbohydrates was assessed by quantifying postprandial cumulative serum 13C-SCFA after a challenge with 13C-inulin.
34224554	10	49	theme	colonic	1674:1680	arg1	fermentation					1682:1693	colonic fermentation	1674:1693	colonic fermentation of 13C-inulin	1674:1707	WB RPS did not enhance colonic fermentation of 13C-inulin and did not affect microbiota composition.
34224554	8	50	theme	serum	1320:1324	arg1	P < 0.05					1335:1342	fasting serum acetate (P < 0.05) and total SCFA (P < 0.05) concentrations	1312:1384	P < 0.05	1335:1342	RESULTS WB RPS increased fasting serum acetate (P < 0.05) and total SCFA (P < 0.05) concentrations in OB participants.
34224554	6	51	theme	stool	960:964	arg1	weight					924:929	fecal dry weight	914:929	fecal dry weight (%)	914:933	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	51	theme	stool	960:964	arg1	permeability					990:1001	intestinal permeability	979:1001	intestinal permeability	979:1001	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	51	theme	stool	960:964	arg1	transit					951:957	transit	951:957	transit	951:957	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	51	theme	stool	960:964	arg1	output					906:911	Fecal output	900:911	Fecal output	900:911	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	51	theme	stool	960:964	arg1	consistency					966:976	stool consistency	960:976	stool consistency	960:976	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	51	theme	stool	960:964	arg1	parameters					1109:1118	health parameters	1102:1118	health parameters	1102:1118	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	51	theme	stool	960:964	arg1	cholesterol					1020:1030	serum total cholesterol	1008:1030	serum total cholesterol	1008:1030	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	51	theme	stool	960:964	arg1	excretion					940:948	fat excretion	936:948	fat excretion	936:948	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	3	52	theme	reduced	490:496	arg1	size					507:510	reduced particle size	490:510	reduced particle size (WB RPS)	490:519	OBJECTIVES We investigated the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health in normal-weight (NW) and obese (OB) participants compared with placebo (PL).
34224554	3	52	theme	reduced	490:496	arg1	RPS					516:518	WB RPS	513:518	WB RPS	513:518	OBJECTIVES We investigated the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health in normal-weight (NW) and obese (OB) participants compared with placebo (PL).
34224554	7	53	from	impact	1125:1130	arg1	fermentation					1149:1160	the fermentation	1145:1160	the fermentation of other carbohydrates	1145:1183	The impact of WB RPS on the fermentation of other carbohydrates was assessed by quantifying postprandial cumulative serum 13C-SCFA after a challenge with 13C-inulin.
34224554	3	54	theme	WB	513:514	arg1	size					507:510	reduced particle size	490:510	reduced particle size (WB RPS)	490:519	OBJECTIVES We investigated the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health in normal-weight (NW) and obese (OB) participants compared with placebo (PL).
34224554	3	54	theme	WB	513:514	arg1	RPS					516:518	WB RPS	513:518	WB RPS	513:518	OBJECTIVES We investigated the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health in normal-weight (NW) and obese (OB) participants compared with placebo (PL).
34224554	3	55	dep	normal-weight	564:576	arg1	NW					579:580	NW	579:580	NW	579:580	OBJECTIVES We investigated the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health in normal-weight (NW) and obese (OB) participants compared with placebo (PL).
34224554	10	56	theme	microbiota	1728:1737	arg1	composition					1739:1749	microbiota composition	1728:1749	microbiota composition	1728:1749	WB RPS did not enhance colonic fermentation of 13C-inulin and did not affect microbiota composition.
34224554	3	57	with	WB	482:483	arg1	size					507:510	reduced particle size	490:510	reduced particle size (WB RPS)	490:519	OBJECTIVES We investigated the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health in normal-weight (NW) and obese (OB) participants compared with placebo (PL).
34224554	3	57	with	WB	482:483	arg1	RPS					516:518	WB RPS	513:518	WB RPS	513:518	OBJECTIVES We investigated the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health in normal-weight (NW) and obese (OB) participants compared with placebo (PL).
34224554	4	58	dep	g	707:707	arg1	RPS					712:714	WB RPS	709:714	WB RPS	709:714	METHODS During 1 mo, 36 NW and 14 OB participants daily consumed 20 g WB RPS or PL (maltodextrin).
34224554	6	59	theme	total	1014:1018	arg1	weight					924:929	fecal dry weight	914:929	fecal dry weight (%)	914:933	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	59	theme	total	1014:1018	arg1	permeability					990:1001	intestinal permeability	979:1001	intestinal permeability	979:1001	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	59	theme	total	1014:1018	arg1	transit					951:957	transit	951:957	transit	951:957	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	59	theme	total	1014:1018	arg1	output					906:911	Fecal output	900:911	Fecal output	900:911	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	59	theme	total	1014:1018	arg1	consistency					966:976	stool consistency	960:976	stool consistency	960:976	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	59	theme	total	1014:1018	arg1	parameters					1109:1118	health parameters	1102:1118	health parameters	1102:1118	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	59	theme	total	1014:1018	arg1	cholesterol					1020:1030	serum total cholesterol	1008:1030	serum total cholesterol	1008:1030	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	59	theme	total	1014:1018	arg1	excretion					940:948	fat excretion	936:948	fat excretion	936:948	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	2	60	theme	particle	293:300	arg1	size					302:305	the particle size	289:305	the particle size of WB	289:311	Reducing the particle size of WB might increase its fermentability and facilitate cross-feeding between the gut bacteria and in this way produce health effects.
34224554	11	61	theme	OB	1835:1836	arg1	participants					1838:1849	OB participants	1835:1849	OB participants	1835:1849	Health parameters were not affected by the WB RPS intervention, either in NW or in OB participants.
34224554	1	62	theme	Wheat	163:167	arg1	WB					175:176	WB	175:176	WB	175:176	BACKGROUND Wheat bran (WB) has been associated with improved gastrointestinal health and a reduced risk of metabolic disorders.
34224554	1	62	theme	Wheat	163:167	arg1	bran					169:172	BACKGROUND Wheat bran	152:172	BACKGROUND Wheat bran (WB)	152:177	BACKGROUND Wheat bran (WB) has been associated with improved gastrointestinal health and a reduced risk of metabolic disorders.
34224554	9	63	theme	Fasting	1406:1412	arg1	concentrations					1431:1444	Fasting serum propionate concentrations	1406:1444	Fasting serum propionate concentrations	1406:1444	Fasting serum propionate concentrations were lower in OB than in NW participants at baseline (NW: 1.57 ± 0.75 µmol/L; OB: 0.89 ± 0.52 µmol/L; P < 0.01), but not after WB RPS (NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant).
34224554	12	64	theme	SCFA	1895:1898	arg1	concentrations					1900:1913	fasting serum SCFA concentrations	1881:1913	fasting serum SCFA concentrations in OB participants	1881:1932	CONCLUSIONS WB RPS increased fasting serum SCFA concentrations in OB participants.
34224554	0	65	theme	maltodextrin	130:141	arg1	placebo					143:149	a maltodextrin placebo	128:149	a maltodextrin placebo	128:149	Wheat bran with reduced particle size increases serum SCFAs in obese subjects without improving health parameters compared with a maltodextrin placebo.
34224554	3	66	theme	normal-weight	564:576	arg1	participants					598:609	normal-weight (NW) and obese (OB) participants	564:609	normal-weight (NW) and obese (OB) participants compared with placebo (PL)	564:636	OBJECTIVES We investigated the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health in normal-weight (NW) and obese (OB) participants compared with placebo (PL).
34224554	6	67	theme	health	1102:1107	arg1	weight					924:929	fecal dry weight	914:929	fecal dry weight (%)	914:933	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	67	theme	health	1102:1107	arg1	permeability					990:1001	intestinal permeability	979:1001	intestinal permeability	979:1001	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	67	theme	health	1102:1107	arg1	transit					951:957	transit	951:957	transit	951:957	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	67	theme	health	1102:1107	arg1	output					906:911	Fecal output	900:911	Fecal output	900:911	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	67	theme	health	1102:1107	arg1	consistency					966:976	stool consistency	960:976	stool consistency	960:976	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	67	theme	health	1102:1107	arg1	parameters					1109:1118	health parameters	1102:1118	health parameters	1102:1118	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	67	theme	health	1102:1107	arg1	cholesterol					1020:1030	serum total cholesterol	1008:1030	serum total cholesterol	1008:1030	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	67	theme	health	1102:1107	arg1	excretion					940:948	fat excretion	936:948	fat excretion	936:948	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	8	68	theme	total	1349:1353	arg1	concentrations					1371:1384	fasting serum acetate (P < 0.05) and total SCFA (P < 0.05) concentrations	1312:1384	concentrations	1371:1384	RESULTS WB RPS increased fasting serum acetate (P < 0.05) and total SCFA (P < 0.05) concentrations in OB participants.
34224554	3	69	dep	OBJECTIVES	441:450	arg1	investigated					455:466	investigated	455:466	investigated the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health in normal-weight (NW) and obese (OB) participants compared with placebo (PL)	455:636	OBJECTIVES We investigated the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health in normal-weight (NW) and obese (OB) participants compared with placebo (PL).
34224554	10	70	theme	WB	1651:1652	arg1	RPS					1654:1656	WB RPS	1651:1656	WB RPS	1651:1656	WB RPS did not enhance colonic fermentation of 13C-inulin and did not affect microbiota composition.
34224554	5	71	theme	fecal	804:808	arg1	SCFAs					797:801	fecal SCFAs	791:801	fecal SCFAs	791:801	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	71	theme	fecal	804:808	arg1	profiles					821:828	fecal metabolite profiles	804:828	fecal metabolite profiles	804:828	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	71	theme	fecal	804:808	arg1	parameters					888:897	fermentation parameters	875:897	fermentation parameters	875:897	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	71	theme	fecal	804:808	arg1	composition					846:856	microbiota composition	835:856	microbiota composition	835:856	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	71	theme	fecal	804:808	arg1	serum					781:785	fasting serum	773:785	fasting serum	773:785	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	3	72	theme	colonic	524:530	arg1	fermentation					532:543	colonic fermentation	524:543	colonic fermentation	524:543	OBJECTIVES We investigated the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health in normal-weight (NW) and obese (OB) participants compared with placebo (PL).
34224554	6	73	theme	dry	920:922	arg1	weight					924:929	fecal dry weight	914:929	fecal dry weight (%)	914:933	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	73	theme	dry	920:922	arg1	permeability					990:1001	intestinal permeability	979:1001	intestinal permeability	979:1001	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	73	theme	dry	920:922	arg1	%					932:932	%	932:932	%	932:932	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	73	theme	dry	920:922	arg1	transit					951:957	transit	951:957	transit	951:957	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	73	theme	dry	920:922	arg1	output					906:911	Fecal output	900:911	Fecal output	900:911	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	73	theme	dry	920:922	arg1	consistency					966:976	stool consistency	960:976	stool consistency	960:976	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	73	theme	dry	920:922	arg1	parameters					1109:1118	health parameters	1102:1118	health parameters	1102:1118	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	73	theme	dry	920:922	arg1	cholesterol					1020:1030	serum total cholesterol	1008:1030	serum total cholesterol	1008:1030	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	73	theme	dry	920:922	arg1	excretion					940:948	fat excretion	936:948	fat excretion	936:948	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	12	74	theme	fasting	1881:1887	arg1	concentrations					1900:1913	fasting serum SCFA concentrations	1881:1913	fasting serum SCFA concentrations in OB participants	1881:1932	CONCLUSIONS WB RPS increased fasting serum SCFA concentrations in OB participants.
34224554	6	75	theme	intestinal	979:988	arg1	weight					924:929	fecal dry weight	914:929	fecal dry weight (%)	914:933	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	75	theme	intestinal	979:988	arg1	permeability					990:1001	intestinal permeability	979:1001	intestinal permeability	979:1001	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	75	theme	intestinal	979:988	arg1	transit					951:957	transit	951:957	transit	951:957	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	75	theme	intestinal	979:988	arg1	output					906:911	Fecal output	900:911	Fecal output	900:911	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	75	theme	intestinal	979:988	arg1	consistency					966:976	stool consistency	960:976	stool consistency	960:976	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	75	theme	intestinal	979:988	arg1	parameters					1109:1118	health parameters	1102:1118	health parameters	1102:1118	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	75	theme	intestinal	979:988	arg1	cholesterol					1020:1030	serum total cholesterol	1008:1030	serum total cholesterol	1008:1030	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	75	theme	intestinal	979:988	arg1	excretion					940:948	fat excretion	936:948	fat excretion	936:948	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	5	76	theme	fasting	773:779	arg1	SCFAs					797:801	fecal SCFAs	791:801	fecal SCFAs	791:801	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	76	theme	fasting	773:779	arg1	profiles					821:828	fecal metabolite profiles	804:828	fecal metabolite profiles	804:828	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	76	theme	fasting	773:779	arg1	serum					781:785	fasting serum	773:785	fasting serum	773:785	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	76	theme	fasting	773:779	arg1	composition					846:856	microbiota composition	835:856	microbiota composition	835:856	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	76	theme	fasting	773:779	arg1	parameters					888:897	fermentation parameters	875:897	fermentation parameters	875:897	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	7	77	theme	cumulative	1226:1235	arg1	13C-SCFA					1243:1250	postprandial cumulative serum 13C-SCFA	1213:1250	postprandial cumulative serum 13C-SCFA	1213:1250	The impact of WB RPS on the fermentation of other carbohydrates was assessed by quantifying postprandial cumulative serum 13C-SCFA after a challenge with 13C-inulin.
34224554	13	78	theme	beneficial	1974:1983	arg1	effects					1985:1991	beneficial effects	1974:1991	beneficial effects on host health	1974:2006	These changes were not associated with beneficial effects on host health.
34224554	1	79	theme	BACKGROUND	152:161	arg1	WB					175:176	WB	175:176	WB	175:176	BACKGROUND Wheat bran (WB) has been associated with improved gastrointestinal health and a reduced risk of metabolic disorders.
34224554	1	79	theme	BACKGROUND	152:161	arg1	bran					169:172	BACKGROUND Wheat bran	152:172	BACKGROUND Wheat bran (WB)	152:177	BACKGROUND Wheat bran (WB) has been associated with improved gastrointestinal health and a reduced risk of metabolic disorders.
34224554	6	80	theme	Fecal	900:904	arg1	weight					924:929	fecal dry weight	914:929	fecal dry weight (%)	914:933	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	80	theme	Fecal	900:904	arg1	permeability					990:1001	intestinal permeability	979:1001	intestinal permeability	979:1001	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	80	theme	Fecal	900:904	arg1	transit					951:957	transit	951:957	transit	951:957	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	80	theme	Fecal	900:904	arg1	output					906:911	Fecal output	900:911	Fecal output	900:911	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	80	theme	Fecal	900:904	arg1	consistency					966:976	stool consistency	960:976	stool consistency	960:976	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	80	theme	Fecal	900:904	arg1	parameters					1109:1118	health parameters	1102:1118	health parameters	1102:1118	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	80	theme	Fecal	900:904	arg1	cholesterol					1020:1030	serum total cholesterol	1008:1030	serum total cholesterol	1008:1030	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	80	theme	Fecal	900:904	arg1	excretion					940:948	fat excretion	936:948	fat excretion	936:948	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	12	81	theme	CONCLUSIONS	1852:1862	arg1	RPS					1867:1869	CONCLUSIONS WB RPS	1852:1869	CONCLUSIONS WB RPS	1852:1869	CONCLUSIONS WB RPS increased fasting serum SCFA concentrations in OB participants.
34224554	9	82	theme	serum	1414:1418	arg1	concentrations					1431:1444	Fasting serum propionate concentrations	1406:1444	Fasting serum propionate concentrations	1406:1444	Fasting serum propionate concentrations were lower in OB than in NW participants at baseline (NW: 1.57 ± 0.75 µmol/L; OB: 0.89 ± 0.52 µmol/L; P < 0.01), but not after WB RPS (NW: 1.75 ± 0.77 µmol/L; OB: 1.35 ± 0.63 µmol/L; P = not significant).
34224554	4	83	theme	OB	673:674	arg1	participants					676:687	14 OB participants	670:687	14 OB participants	670:687	METHODS During 1 mo, 36 NW and 14 OB participants daily consumed 20 g WB RPS or PL (maltodextrin).
34224554	7	84	with	challenge	1260:1268	arg1	13C-inulin					1275:1284	13C-inulin	1275:1284	13C-inulin	1275:1284	The impact of WB RPS on the fermentation of other carbohydrates was assessed by quantifying postprandial cumulative serum 13C-SCFA after a challenge with 13C-inulin.
34224554	2	85	theme	gut	388:390	arg1	bacteria					392:399	the gut bacteria	384:399	the gut bacteria	384:399	Reducing the particle size of WB might increase its fermentability and facilitate cross-feeding between the gut bacteria and in this way produce health effects.
34224554	6	86	theme	fat	936:938	arg1	weight					924:929	fecal dry weight	914:929	fecal dry weight (%)	914:933	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	86	theme	fat	936:938	arg1	permeability					990:1001	intestinal permeability	979:1001	intestinal permeability	979:1001	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	86	theme	fat	936:938	arg1	transit					951:957	transit	951:957	transit	951:957	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	86	theme	fat	936:938	arg1	output					906:911	Fecal output	900:911	Fecal output	900:911	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	86	theme	fat	936:938	arg1	consistency					966:976	stool consistency	960:976	stool consistency	960:976	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	86	theme	fat	936:938	arg1	parameters					1109:1118	health parameters	1102:1118	health parameters	1102:1118	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	86	theme	fat	936:938	arg1	cholesterol					1020:1030	serum total cholesterol	1008:1030	serum total cholesterol	1008:1030	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	6	86	theme	fat	936:938	arg1	excretion					940:948	fat excretion	936:948	fat excretion	936:948	Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters.
34224554	0	87	theme	reduced	16:22	arg1	size					33:36	reduced particle size	16:36	reduced particle size	16:36	Wheat bran with reduced particle size increases serum SCFAs in obese subjects without improving health parameters compared with a maltodextrin placebo.
34224554	1	88	theme	disorders	269:277	arg1	health					230:235	improved gastrointestinal health	204:235	improved gastrointestinal health	204:235	BACKGROUND Wheat bran (WB) has been associated with improved gastrointestinal health and a reduced risk of metabolic disorders.
34224554	1	88	theme	disorders	269:277	arg1	risk					251:254	a reduced risk	241:254	a reduced risk of metabolic disorders	241:277	BACKGROUND Wheat bran (WB) has been associated with improved gastrointestinal health and a reduced risk of metabolic disorders.
34224554	7	89	theme	other	1165:1169	arg1	carbohydrates					1171:1183	other carbohydrates	1165:1183	other carbohydrates	1165:1183	The impact of WB RPS on the fermentation of other carbohydrates was assessed by quantifying postprandial cumulative serum 13C-SCFA after a challenge with 13C-inulin.
34224554	2	90	dep	increase	319:326	arg1	produce					417:423	produce	417:423	produce health effects	417:438	Reducing the particle size of WB might increase its fermentability and facilitate cross-feeding between the gut bacteria and in this way produce health effects.
34224554	11	91	theme	Health	1752:1757	arg1	parameters					1759:1768	Health parameters	1752:1768	Health parameters	1752:1768	Health parameters were not affected by the WB RPS intervention, either in NW or in OB participants.
34224554	12	92	theme	OB	1918:1919	arg1	participants					1921:1932	OB participants	1918:1932	OB participants	1918:1932	CONCLUSIONS WB RPS increased fasting serum SCFA concentrations in OB participants.
34224554	0	93	theme	serum	48:52	arg1	SCFAs					54:58	serum SCFAs	48:58	serum SCFAs in obese subjects	48:76	Wheat bran with reduced particle size increases serum SCFAs in obese subjects without improving health parameters compared with a maltodextrin placebo.
34224554	10	94	theme	13C-inulin	1698:1707	arg1	fermentation					1682:1693	colonic fermentation	1674:1693	colonic fermentation of 13C-inulin	1674:1707	WB RPS did not enhance colonic fermentation of 13C-inulin and did not affect microbiota composition.
34224554	8	95	theme	RESULTS	1287:1293	arg1	RPS					1298:1300	RESULTS WB RPS	1287:1300	RESULTS WB RPS	1287:1300	RESULTS WB RPS increased fasting serum acetate (P < 0.05) and total SCFA (P < 0.05) concentrations in OB participants.
34224554	0	96	with	bran	6:9	arg1	size					33:36	reduced particle size	16:36	reduced particle size	16:36	Wheat bran with reduced particle size increases serum SCFAs in obese subjects without improving health parameters compared with a maltodextrin placebo.
34224554	4	97	theme	WB	709:710	arg1	RPS					712:714	WB RPS	709:714	WB RPS	709:714	METHODS During 1 mo, 36 NW and 14 OB participants daily consumed 20 g WB RPS or PL (maltodextrin).
34224554	5	98	theme	fermentation	875:886	arg1	SCFAs					797:801	fecal SCFAs	791:801	fecal SCFAs	791:801	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	98	theme	fermentation	875:886	arg1	profiles					821:828	fecal metabolite profiles	804:828	fecal metabolite profiles	804:828	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	98	theme	fermentation	875:886	arg1	parameters					888:897	fermentation parameters	875:897	fermentation parameters	875:897	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	98	theme	fermentation	875:886	arg1	composition					846:856	microbiota composition	835:856	microbiota composition	835:856	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	5	98	theme	fermentation	875:886	arg1	serum					781:785	fasting serum	773:785	fasting serum	773:785	Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters.
34224554	7	99	theme	WB	1135:1136	arg1	RPS					1138:1140	WB RPS	1135:1140	WB RPS	1135:1140	The impact of WB RPS on the fermentation of other carbohydrates was assessed by quantifying postprandial cumulative serum 13C-SCFA after a challenge with 13C-inulin.
34224554	13	100	from	effects	1985:1991	arg1	health					2001:2006	host health	1996:2006	host health	1996:2006	These changes were not associated with beneficial effects on host health.
34224554	2	101	theme	WB	310:311	arg1	size					302:305	the particle size	289:305	the particle size of WB	289:311	Reducing the particle size of WB might increase its fermentability and facilitate cross-feeding between the gut bacteria and in this way produce health effects.
34224554	1	102	theme	gastrointestinal	213:228	arg1	health					230:235	improved gastrointestinal health	204:235	improved gastrointestinal health	204:235	BACKGROUND Wheat bran (WB) has been associated with improved gastrointestinal health and a reduced risk of metabolic disorders.
34224554	8	103	theme	fasting	1312:1318	arg1	P < 0.05					1335:1342	fasting serum acetate (P < 0.05) and total SCFA (P < 0.05) concentrations	1312:1384	P < 0.05	1335:1342	RESULTS WB RPS increased fasting serum acetate (P < 0.05) and total SCFA (P < 0.05) concentrations in OB participants.
34224554	13	104	theme	host	1996:1999	arg1	health					2001:2006	host health	1996:2006	host health	1996:2006	These changes were not associated with beneficial effects on host health.
33836190	10	0	theme	composite	1141:1149	arg1	beads					1151:1155	The composite beads	1137:1155	The composite beads	1137:1155	The composite beads also showed good reusability and biodegradability.
33836190	0	1	with	beads	44:48	arg1	structure					70:78	aligned porous structure	55:78	aligned porous structure for fast methylene blue adsorption	55:113	Hollow cellulose-carbon nanotubes composite beads with aligned porous structure for fast methylene blue adsorption.
33836190	4	2	theme	cellulose	660:668	arg1	beads					670:674	the cellulose beads	656:674	the cellulose beads	656:674	Different characterizations have proved that the carbon nanotubes and magnetic nanoparticles have been incorporated into the cellulose beads.
33836190	0	3	theme	fast	84:87	arg1	adsorption					104:113	fast methylene blue adsorption	84:113	fast methylene blue adsorption	84:113	Hollow cellulose-carbon nanotubes composite beads with aligned porous structure for fast methylene blue adsorption.
33836190	4	4	theme	carbon	584:589	arg1	nanotubes					591:599	the carbon nanotubes	580:599	the carbon nanotubes	580:599	Different characterizations have proved that the carbon nanotubes and magnetic nanoparticles have been incorporated into the cellulose beads.
33836190	5	5	theme	Higher	677:682	arg1	concentration					684:696	Higher concentration	677:696	Higher concentration of carbon nanotubes and cellulose	677:730	Higher concentration of carbon nanotubes and cellulose would result in a larger diameter of the composite beads.
33836190	11	6	theme	adsorption	1364:1373	arg1	fields					1375:1380	adsorption fields	1364:1380	adsorption fields	1364:1380	We anticipate that different polysaccharides based composite beads with aligned porous structure can be obtained through the similar methods and applied in adsorption fields.
33836190	2	7	theme	hollow	313:318	arg1	nanotubes					337:345	hollow cellulose/carbon nanotubes	313:345	hollow cellulose/carbon nanotubes composite beads with aligned porous structure which have potential applications in fast adsorption field	313:450	The purpose of this paper was to develop hollow cellulose/carbon nanotubes composite beads with aligned porous structure which have potential applications in fast adsorption field.
33836190	1	8	theme	Polysaccharide	116:129	arg1	beads					137:141	Polysaccharide based beads	116:141	Polysaccharide based beads with unique porous structure	116:170	Polysaccharide based beads with unique porous structure have gained considerable interests due to their specific adsorption behaviors and biodegradability.
33836190	2	9	with	beads	357:361	arg1	structure					383:391	aligned porous structure	368:391	aligned porous structure	368:391	The purpose of this paper was to develop hollow cellulose/carbon nanotubes composite beads with aligned porous structure which have potential applications in fast adsorption field.
33836190	4	10	theme	Different	535:543	arg1	characterizations					545:561	Different characterizations	535:561	Different characterizations	535:561	Different characterizations have proved that the carbon nanotubes and magnetic nanoparticles have been incorporated into the cellulose beads.
33836190	5	11	theme	larger	750:755	arg1	diameter					757:764	a larger diameter	748:764	a larger diameter of the composite beads	748:787	Higher concentration of carbon nanotubes and cellulose would result in a larger diameter of the composite beads.
33836190	6	12	theme	composite	794:802	arg1	beads					804:808	The composite beads	790:808	The composite beads	790:808	The composite beads can effectively adsorb the methylene blue (MB).
33836190	2	13	contain	have	399:402	arg2	applications					414:425	potential applications	404:425	potential applications	404:425	The purpose of this paper was to develop hollow cellulose/carbon nanotubes composite beads with aligned porous structure which have potential applications in fast adsorption field.
33836190	2	13	contain	have	399:402	arg1	beads					357:361	composite beads	347:361	composite beads with aligned porous structure	347:391	The purpose of this paper was to develop hollow cellulose/carbon nanotubes composite beads with aligned porous structure which have potential applications in fast adsorption field.
33836190	5	14	theme	nanotubes	708:716	arg1	concentration					684:696	Higher concentration	677:696	Higher concentration of carbon nanotubes and cellulose	677:730	Higher concentration of carbon nanotubes and cellulose would result in a larger diameter of the composite beads.
33836190	5	15	theme	carbon	701:706	arg1	nanotubes					708:716	carbon nanotubes	701:716	carbon nanotubes	701:716	Higher concentration of carbon nanotubes and cellulose would result in a larger diameter of the composite beads.
33836190	1	16	theme	considerable	184:195	arg1	interests					197:205	considerable interests	184:205	considerable interests	184:205	Polysaccharide based beads with unique porous structure have gained considerable interests due to their specific adsorption behaviors and biodegradability.
33836190	9	17	theme	adsorption	1088:1097	arg1	capacity					1099:1106	The maximum adsorption capacity	1076:1106	The maximum adsorption capacity	1076:1106	The maximum adsorption capacity was 285.71 mg g-1 at pH 7.0.
33836190	9	17	theme	adsorption	1088:1097	arg1	g-1					1122:1124	285.71 mg g-1	1112:1124	285.71 mg g-1	1112:1124	The maximum adsorption capacity was 285.71 mg g-1 at pH 7.0.
33836190	11	18	theme	porous	1288:1293	arg1	structure					1295:1303	aligned porous structure	1280:1303	aligned porous structure	1280:1303	We anticipate that different polysaccharides based composite beads with aligned porous structure can be obtained through the similar methods and applied in adsorption fields.
33836190	11	19	theme	composite	1259:1267	arg1	beads					1269:1273	composite beads	1259:1273	composite beads with aligned porous structure	1259:1303	We anticipate that different polysaccharides based composite beads with aligned porous structure can be obtained through the similar methods and applied in adsorption fields.
33836190	2	20	theme	adsorption	435:444	arg1	field					446:450	fast adsorption field	430:450	fast adsorption field	430:450	The purpose of this paper was to develop hollow cellulose/carbon nanotubes composite beads with aligned porous structure which have potential applications in fast adsorption field.
33836190	5	21	theme	cellulose	722:730	arg1	concentration					684:696	Higher concentration	677:696	Higher concentration of carbon nanotubes and cellulose	677:730	Higher concentration of carbon nanotubes and cellulose would result in a larger diameter of the composite beads.
33836190	8	22	theme	composite	950:958	arg1	beads					960:964	The composite beads	946:964	The composite beads	946:964	The composite beads showed a fast adsorption behavior towards MB with a t1/2 of 1.07 min obtained from pseudo-second-order model.
33836190	10	23	theme	good	1169:1172	arg1	reusability					1174:1184	good reusability	1169:1184	good reusability	1169:1184	The composite beads also showed good reusability and biodegradability.
33836190	2	24	theme	fast	430:433	arg1	field					446:450	fast adsorption field	430:450	fast adsorption field	430:450	The purpose of this paper was to develop hollow cellulose/carbon nanotubes composite beads with aligned porous structure which have potential applications in fast adsorption field.
33836190	0	25	theme	Hollow	0:5	arg1	cellulose-carbon					7:22	Hollow cellulose-carbon	0:22	Hollow cellulose-carbon	0:22	Hollow cellulose-carbon nanotubes composite beads with aligned porous structure for fast methylene blue adsorption.
33836190	1	26	with	beads	137:141	arg1	structure					162:170	unique porous structure	148:170	unique porous structure	148:170	Polysaccharide based beads with unique porous structure have gained considerable interests due to their specific adsorption behaviors and biodegradability.
33836190	0	27	dep	fast	84:87	arg1	blue					99:102	blue	99:102	blue	99:102	Hollow cellulose-carbon nanotubes composite beads with aligned porous structure for fast methylene blue adsorption.
33836190	8	28	theme	fast	975:978	arg1	behavior					991:998	a fast adsorption behavior	973:998	a fast adsorption behavior towards MB	973:1009	The composite beads showed a fast adsorption behavior towards MB with a t1/2 of 1.07 min obtained from pseudo-second-order model.
33836190	0	29	theme	composite	34:42	arg1	beads					44:48	composite beads	34:48	composite beads with aligned porous structure for fast methylene blue adsorption	34:113	Hollow cellulose-carbon nanotubes composite beads with aligned porous structure for fast methylene blue adsorption.
33836190	7	30	theme	Langmuir	892:899	arg1	isotherm					901:908	Langmuir isotherm	892:908	Langmuir isotherm	892:908	The pseudo-second-order model and Langmuir isotherm were best fitted to the adsorption.
33836190	5	31	theme	beads	783:787	arg1	diameter					757:764	a larger diameter	748:764	a larger diameter of the composite beads	748:787	Higher concentration of carbon nanotubes and cellulose would result in a larger diameter of the composite beads.
33836190	8	32	theme	pseudo-second-order	1049:1067	arg1	model					1069:1073	pseudo-second-order model	1049:1073	pseudo-second-order model	1049:1073	The composite beads showed a fast adsorption behavior towards MB with a t1/2 of 1.07 min obtained from pseudo-second-order model.
33836190	11	33	theme	aligned	1280:1286	arg1	structure					1295:1303	aligned porous structure	1280:1303	aligned porous structure	1280:1303	We anticipate that different polysaccharides based composite beads with aligned porous structure can be obtained through the similar methods and applied in adsorption fields.
33836190	3	34	theme	freeze-drying	509:521	arg1	technology					523:532	freeze-drying technology	509:532	freeze-drying technology	509:532	The composite beads were fabricated by ice template and freeze-drying technology.
33836190	2	35	theme	composite	347:355	arg1	beads					357:361	composite beads	347:361	composite beads with aligned porous structure	347:391	The purpose of this paper was to develop hollow cellulose/carbon nanotubes composite beads with aligned porous structure which have potential applications in fast adsorption field.
33836190	7	36	theme	pseudo-second-order	862:880	arg1	model					882:886	The pseudo-second-order model	858:886	The pseudo-second-order model	858:886	The pseudo-second-order model and Langmuir isotherm were best fitted to the adsorption.
33836190	2	37	theme	cellulose/carbon	320:335	arg1	nanotubes					337:345	hollow cellulose/carbon nanotubes	313:345	hollow cellulose/carbon nanotubes composite beads with aligned porous structure which have potential applications in fast adsorption field	313:450	The purpose of this paper was to develop hollow cellulose/carbon nanotubes composite beads with aligned porous structure which have potential applications in fast adsorption field.
33836190	1	38	theme	specific	220:227	arg1	behaviors					240:248	their specific adsorption behaviors	214:248	their specific adsorption behaviors	214:248	Polysaccharide based beads with unique porous structure have gained considerable interests due to their specific adsorption behaviors and biodegradability.
33836190	3	39	theme	composite	457:465	arg1	beads					467:471	The composite beads	453:471	The composite beads	453:471	The composite beads were fabricated by ice template and freeze-drying technology.
33836190	8	40	theme	adsorption	980:989	arg1	behavior					991:998	a fast adsorption behavior	973:998	a fast adsorption behavior towards MB	973:1009	The composite beads showed a fast adsorption behavior towards MB with a t1/2 of 1.07 min obtained from pseudo-second-order model.
33836190	1	41	theme	adsorption	229:238	arg1	behaviors					240:248	their specific adsorption behaviors	214:248	their specific adsorption behaviors	214:248	Polysaccharide based beads with unique porous structure have gained considerable interests due to their specific adsorption behaviors and biodegradability.
33836190	11	42	with	beads	1269:1273	arg1	structure					1295:1303	aligned porous structure	1280:1303	aligned porous structure	1280:1303	We anticipate that different polysaccharides based composite beads with aligned porous structure can be obtained through the similar methods and applied in adsorption fields.
33836190	0	43	theme	porous	63:68	arg1	structure					70:78	aligned porous structure	55:78	aligned porous structure for fast methylene blue adsorption	55:113	Hollow cellulose-carbon nanotubes composite beads with aligned porous structure for fast methylene blue adsorption.
33836190	6	44	theme	blue	847:850	arg1	methylene					837:845	methylene blue	837:850	the methylene blue (MB)	833:855	The composite beads can effectively adsorb the methylene blue (MB).
33836190	6	44	theme	blue	847:850	arg1	MB					853:854	MB	853:854	MB	853:854	The composite beads can effectively adsorb the methylene blue (MB).
33836190	1	45	theme	based	131:135	arg1	beads					137:141	Polysaccharide based beads	116:141	Polysaccharide based beads with unique porous structure	116:170	Polysaccharide based beads with unique porous structure have gained considerable interests due to their specific adsorption behaviors and biodegradability.
33836190	0	46	theme	aligned	55:61	arg1	structure					70:78	aligned porous structure	55:78	aligned porous structure for fast methylene blue adsorption	55:113	Hollow cellulose-carbon nanotubes composite beads with aligned porous structure for fast methylene blue adsorption.
33836190	2	47	theme	porous	376:381	arg1	structure					383:391	aligned porous structure	368:391	aligned porous structure	368:391	The purpose of this paper was to develop hollow cellulose/carbon nanotubes composite beads with aligned porous structure which have potential applications in fast adsorption field.
33836190	4	48	theme	magnetic	605:612	arg1	nanoparticles					614:626	magnetic nanoparticles	605:626	magnetic nanoparticles	605:626	Different characterizations have proved that the carbon nanotubes and magnetic nanoparticles have been incorporated into the cellulose beads.
33836190	11	49	theme	similar	1333:1339	arg1	methods					1341:1347	the similar methods	1329:1347	the similar methods	1329:1347	We anticipate that different polysaccharides based composite beads with aligned porous structure can be obtained through the similar methods and applied in adsorption fields.
33836190	11	50	theme	different	1227:1235	arg1	polysaccharides					1237:1251	different polysaccharides	1227:1251	different polysaccharides based composite beads with aligned porous structure	1227:1303	We anticipate that different polysaccharides based composite beads with aligned porous structure can be obtained through the similar methods and applied in adsorption fields.
33836190	2	51	theme	paper	292:296	arg1	purpose					276:282	The purpose	272:282	The purpose of this paper	272:296	The purpose of this paper was to develop hollow cellulose/carbon nanotubes composite beads with aligned porous structure which have potential applications in fast adsorption field.
33836190	2	52	theme	aligned	368:374	arg1	structure					383:391	aligned porous structure	368:391	aligned porous structure	368:391	The purpose of this paper was to develop hollow cellulose/carbon nanotubes composite beads with aligned porous structure which have potential applications in fast adsorption field.
33836190	5	53	theme	composite	773:781	arg1	beads					783:787	the composite beads	769:787	the composite beads	769:787	Higher concentration of carbon nanotubes and cellulose would result in a larger diameter of the composite beads.
33836190	9	54	from	pH	1129:1130	arg1	capacity					1099:1106	The maximum adsorption capacity	1076:1106	The maximum adsorption capacity	1076:1106	The maximum adsorption capacity was 285.71 mg g-1 at pH 7.0.
33836190	9	54	from	pH	1129:1130	arg1	g-1					1122:1124	285.71 mg g-1	1112:1124	285.71 mg g-1	1112:1124	The maximum adsorption capacity was 285.71 mg g-1 at pH 7.0.
33836190	1	55	theme	unique	148:153	arg1	structure					162:170	unique porous structure	148:170	unique porous structure	148:170	Polysaccharide based beads with unique porous structure have gained considerable interests due to their specific adsorption behaviors and biodegradability.
33836190	9	56	theme	285.71	1112:1117	arg1	mg					1119:1120	mg	1119:1120	mg	1119:1120	The maximum adsorption capacity was 285.71 mg g-1 at pH 7.0.
33836190	2	57	dep	beads	357:361	arg1	nanotubes					337:345	hollow cellulose/carbon nanotubes	313:345	hollow cellulose/carbon nanotubes composite beads with aligned porous structure which have potential applications in fast adsorption field	313:450	The purpose of this paper was to develop hollow cellulose/carbon nanotubes composite beads with aligned porous structure which have potential applications in fast adsorption field.
33836190	1	58	theme	porous	155:160	arg1	structure					162:170	unique porous structure	148:170	unique porous structure	148:170	Polysaccharide based beads with unique porous structure have gained considerable interests due to their specific adsorption behaviors and biodegradability.
33836190	3	59	theme	ice	492:494	arg1	template					496:503	ice template	492:503	ice template	492:503	The composite beads were fabricated by ice template and freeze-drying technology.
33836190	9	60	theme	maximum	1080:1086	arg1	capacity					1099:1106	The maximum adsorption capacity	1076:1106	The maximum adsorption capacity	1076:1106	The maximum adsorption capacity was 285.71 mg g-1 at pH 7.0.
33836190	9	60	theme	maximum	1080:1086	arg1	g-1					1122:1124	285.71 mg g-1	1112:1124	285.71 mg g-1	1112:1124	The maximum adsorption capacity was 285.71 mg g-1 at pH 7.0.
33836190	9	61	theme	mg	1119:1120	arg1	capacity					1099:1106	The maximum adsorption capacity	1076:1106	The maximum adsorption capacity	1076:1106	The maximum adsorption capacity was 285.71 mg g-1 at pH 7.0.
33836190	9	61	theme	mg	1119:1120	arg1	g-1					1122:1124	285.71 mg g-1	1112:1124	285.71 mg g-1	1112:1124	The maximum adsorption capacity was 285.71 mg g-1 at pH 7.0.
33836190	8	62	theme	min	1031:1033	arg1	t1/2					1018:1021	a t1/2	1016:1021	a t1/2 of 1.07 min obtained from pseudo-second-order model	1016:1073	The composite beads showed a fast adsorption behavior towards MB with a t1/2 of 1.07 min obtained from pseudo-second-order model.
33836190	2	63	theme	potential	404:412	arg1	applications					414:425	potential applications	404:425	potential applications	404:425	The purpose of this paper was to develop hollow cellulose/carbon nanotubes composite beads with aligned porous structure which have potential applications in fast adsorption field.
34044920	0	0	theme	electrostatic	96:108	arg1	interactions					110:121	electrostatic interactions	96:121	electrostatic interactions	96:121	Enhanced physical and antimicrobial properties of alginate/chitosan composite aerogels based on electrostatic interactions and noncovalent crosslinking.
34044920	1	1	theme	alginate/chitosan	172:188	arg1	aerogels					200:207	the alginate/chitosan composite aerogels	168:207	the alginate/chitosan composite aerogels based on electrostatic interactions and noncovalent crosslinking	168:272	In this study, the alginate/chitosan composite aerogels based on electrostatic interactions and noncovalent crosslinking were fabricated using sol-gel method followed by freeze-drying process.
34044920	1	2	theme	composite	190:198	arg1	aerogels					200:207	the alginate/chitosan composite aerogels	168:207	the alginate/chitosan composite aerogels based on electrostatic interactions and noncovalent crosslinking	168:272	In this study, the alginate/chitosan composite aerogels based on electrostatic interactions and noncovalent crosslinking were fabricated using sol-gel method followed by freeze-drying process.
34044920	5	3	theme	electrostatic	1067:1079	arg1	interactions					1081:1092	electrostatic interactions	1067:1092	electrostatic interactions	1067:1092	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	2	4	theme	chitosan	409:416	arg1	addition					397:404	the addition	393:404	the addition of chitosan in alginate solution	393:437	The solution property results showed that with the addition of chitosan in alginate solution, a tighter network was induced by the more entangled molecular chains.
34044920	2	5	theme	solution	350:357	arg1	results					368:374	The solution property results	346:374	The solution property results	346:374	The solution property results showed that with the addition of chitosan in alginate solution, a tighter network was induced by the more entangled molecular chains.
34044920	3	6	theme	chitosan	622:629	arg1	ratio					613:617	the increasing weight ratio	591:617	the increasing weight ratio of chitosan in the aerogels	591:645	The aerogel morphology observations showed that the pore diameter decreased with the increasing weight ratio of chitosan in the aerogels, but was even much lower after the crosslinking of excess alginate with calcium ions.
34044920	5	7	theme	aerogels	1025:1032	arg1	properties					989:998	the enhanced physical and antimicrobial properties	949:998	the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels	949:1032	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	5	8	theme	packaging	1205:1213	arg1	applications					1149:1160	the promising applications	1135:1160	the promising applications of these composite aerogels	1135:1188	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	5	8	theme	packaging	1205:1213	arg1	materials					1215:1223	active food packaging materials	1193:1223	active food packaging materials for antimicrobial purpose	1193:1249	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	4	9	theme	higher	811:816	arg1	properties					829:838	higher mechanical properties	811:838	higher mechanical properties	811:838	After crosslinking, the aerogels presented the improved thermal stability and higher mechanical properties, as well as stronger antibacterial activities against Staphylococcus aureus and Escherichia coli.
34044920	3	10	theme	pore	562:565	arg1	diameter					567:574	the pore diameter	558:574	the pore diameter	558:574	The aerogel morphology observations showed that the pore diameter decreased with the increasing weight ratio of chitosan in the aerogels, but was even much lower after the crosslinking of excess alginate with calcium ions.
34044920	3	10	theme	pore	562:565	arg1	lower					666:670	lower	666:670	lower	666:670	The aerogel morphology observations showed that the pore diameter decreased with the increasing weight ratio of chitosan in the aerogels, but was even much lower after the crosslinking of excess alginate with calcium ions.
34044920	1	11	theme	freeze-drying	323:335	arg1	process					337:343	freeze-drying process	323:343	freeze-drying process	323:343	In this study, the alginate/chitosan composite aerogels based on electrostatic interactions and noncovalent crosslinking were fabricated using sol-gel method followed by freeze-drying process.
34044920	2	12	from	addition	397:404	arg1	solution					430:437	alginate solution	421:437	alginate solution	421:437	The solution property results showed that with the addition of chitosan in alginate solution, a tighter network was induced by the more entangled molecular chains.
34044920	0	13	theme	noncovalent	127:137	arg1	crosslinking					139:150	noncovalent crosslinking	127:150	noncovalent crosslinking	127:150	Enhanced physical and antimicrobial properties of alginate/chitosan composite aerogels based on electrostatic interactions and noncovalent crosslinking.
34044920	5	14	theme	antimicrobial	975:987	arg1	properties					989:998	the enhanced physical and antimicrobial properties	949:998	the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels	949:1032	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	0	15	theme	Enhanced	0:7	arg1	properties					36:45	Enhanced physical and antimicrobial properties	0:45	Enhanced physical and antimicrobial properties of alginate/chitosan composite aerogels	0:85	Enhanced physical and antimicrobial properties of alginate/chitosan composite aerogels based on electrostatic interactions and noncovalent crosslinking.
34044920	2	16	theme	tighter	442:448	arg1	network					450:456	a tighter network	440:456	a tighter network	440:456	The solution property results showed that with the addition of chitosan in alginate solution, a tighter network was induced by the more entangled molecular chains.
34044920	0	17	theme	physical	9:16	arg1	properties					36:45	Enhanced physical and antimicrobial properties	0:45	Enhanced physical and antimicrobial properties of alginate/chitosan composite aerogels	0:85	Enhanced physical and antimicrobial properties of alginate/chitosan composite aerogels based on electrostatic interactions and noncovalent crosslinking.
34044920	5	18	theme	antimicrobial	1229:1241	arg1	purpose					1243:1249	antimicrobial purpose	1229:1249	antimicrobial purpose	1229:1249	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	4	19	theme	thermal	789:795	arg1	stability					797:805	the improved thermal stability	776:805	the improved thermal stability	776:805	After crosslinking, the aerogels presented the improved thermal stability and higher mechanical properties, as well as stronger antibacterial activities against Staphylococcus aureus and Escherichia coli.
34044920	2	20	theme	molecular	492:500	arg1	chains					502:507	the more entangled molecular chains	473:507	the more entangled molecular chains	473:507	The solution property results showed that with the addition of chitosan in alginate solution, a tighter network was induced by the more entangled molecular chains.
34044920	5	21	theme	composite	1171:1179	arg1	aerogels					1181:1188	these composite aerogels	1165:1188	these composite aerogels	1165:1188	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	4	22	theme	improved	780:787	arg1	stability					797:805	the improved thermal stability	776:805	the improved thermal stability	776:805	After crosslinking, the aerogels presented the improved thermal stability and higher mechanical properties, as well as stronger antibacterial activities against Staphylococcus aureus and Escherichia coli.
34044920	4	23	theme	mechanical	818:827	arg1	properties					829:838	higher mechanical properties	811:838	higher mechanical properties	811:838	After crosslinking, the aerogels presented the improved thermal stability and higher mechanical properties, as well as stronger antibacterial activities against Staphylococcus aureus and Escherichia coli.
34044920	1	24	theme	electrostatic	218:230	arg1	interactions					232:243	electrostatic interactions	218:243	electrostatic interactions	218:243	In this study, the alginate/chitosan composite aerogels based on electrostatic interactions and noncovalent crosslinking were fabricated using sol-gel method followed by freeze-drying process.
34044920	2	25	theme	entangled	482:490	arg1	chains					502:507	the more entangled molecular chains	473:507	the more entangled molecular chains	473:507	The solution property results showed that with the addition of chitosan in alginate solution, a tighter network was induced by the more entangled molecular chains.
34044920	5	26	theme	noncovalent	1098:1108	arg1	crosslinking					1110:1121	noncovalent crosslinking	1098:1121	noncovalent crosslinking	1098:1121	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	5	27	theme	alginate/chitosan	1007:1023	arg1	aerogels					1025:1032	the alginate/chitosan aerogels	1003:1032	the alginate/chitosan aerogels	1003:1032	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	3	28	with	crosslinking	682:693	arg1	ions					727:730	calcium ions	719:730	calcium ions	719:730	The aerogel morphology observations showed that the pore diameter decreased with the increasing weight ratio of chitosan in the aerogels, but was even much lower after the crosslinking of excess alginate with calcium ions.
34044920	3	29	theme	aerogel	514:520	arg1	observations					533:544	The aerogel morphology observations	510:544	The aerogel morphology observations	510:544	The aerogel morphology observations showed that the pore diameter decreased with the increasing weight ratio of chitosan in the aerogels, but was even much lower after the crosslinking of excess alginate with calcium ions.
34044920	5	30	theme	aerogels	1181:1188	arg1	applications					1149:1160	the promising applications	1135:1160	the promising applications of these composite aerogels	1135:1188	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	5	30	theme	aerogels	1181:1188	arg1	materials					1215:1223	active food packaging materials	1193:1223	active food packaging materials for antimicrobial purpose	1193:1249	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	2	31	theme	property	359:366	arg1	results					368:374	The solution property results	346:374	The solution property results	346:374	The solution property results showed that with the addition of chitosan in alginate solution, a tighter network was induced by the more entangled molecular chains.
34044920	3	32	theme	morphology	522:531	arg1	observations					533:544	The aerogel morphology observations	510:544	The aerogel morphology observations	510:544	The aerogel morphology observations showed that the pore diameter decreased with the increasing weight ratio of chitosan in the aerogels, but was even much lower after the crosslinking of excess alginate with calcium ions.
34044920	3	33	theme	excess	698:703	arg1	alginate					705:712	excess alginate	698:712	excess alginate	698:712	The aerogel morphology observations showed that the pore diameter decreased with the increasing weight ratio of chitosan in the aerogels, but was even much lower after the crosslinking of excess alginate with calcium ions.
34044920	1	34	theme	noncovalent	249:259	arg1	crosslinking					261:272	noncovalent crosslinking	249:272	noncovalent crosslinking	249:272	In this study, the alginate/chitosan composite aerogels based on electrostatic interactions and noncovalent crosslinking were fabricated using sol-gel method followed by freeze-drying process.
34044920	5	35	theme	active	1193:1198	arg1	applications					1149:1160	the promising applications	1135:1160	the promising applications of these composite aerogels	1135:1188	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	5	35	theme	active	1193:1198	arg1	materials					1215:1223	active food packaging materials	1193:1223	active food packaging materials for antimicrobial purpose	1193:1249	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	0	36	theme	antimicrobial	22:34	arg1	properties					36:45	Enhanced physical and antimicrobial properties	0:45	Enhanced physical and antimicrobial properties of alginate/chitosan composite aerogels	0:85	Enhanced physical and antimicrobial properties of alginate/chitosan composite aerogels based on electrostatic interactions and noncovalent crosslinking.
34044920	0	37	theme	composite	68:76	arg1	aerogels					78:85	alginate/chitosan composite aerogels	50:85	alginate/chitosan composite aerogels	50:85	Enhanced physical and antimicrobial properties of alginate/chitosan composite aerogels based on electrostatic interactions and noncovalent crosslinking.
34044920	5	38	theme	food	1200:1203	arg1	applications					1149:1160	the promising applications	1135:1160	the promising applications of these composite aerogels	1135:1188	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	5	38	theme	food	1200:1203	arg1	materials					1215:1223	active food packaging materials	1193:1223	active food packaging materials for antimicrobial purpose	1193:1249	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	0	39	theme	alginate/chitosan	50:66	arg1	aerogels					78:85	alginate/chitosan composite aerogels	50:85	alginate/chitosan composite aerogels	50:85	Enhanced physical and antimicrobial properties of alginate/chitosan composite aerogels based on electrostatic interactions and noncovalent crosslinking.
34044920	3	40	theme	calcium	719:725	arg1	ions					727:730	calcium ions	719:730	calcium ions	719:730	The aerogel morphology observations showed that the pore diameter decreased with the increasing weight ratio of chitosan in the aerogels, but was even much lower after the crosslinking of excess alginate with calcium ions.
34044920	5	41	theme	promising	1139:1147	arg1	applications					1149:1160	the promising applications	1135:1160	the promising applications of these composite aerogels	1135:1188	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	5	41	theme	promising	1139:1147	arg1	materials					1215:1223	active food packaging materials	1193:1223	active food packaging materials for antimicrobial purpose	1193:1249	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	5	42	theme	interactions	1081:1092	arg1	modulation					1053:1062	modulation	1053:1062	modulation of electrostatic interactions and noncovalent crosslinking	1053:1121	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	4	43	theme	antibacterial	861:873	arg1	activities					875:884	stronger antibacterial activities	852:884	stronger antibacterial activities against Staphylococcus aureus and Escherichia coli	852:935	After crosslinking, the aerogels presented the improved thermal stability and higher mechanical properties, as well as stronger antibacterial activities against Staphylococcus aureus and Escherichia coli.
34044920	3	44	theme	increasing	595:604	arg1	ratio					613:617	the increasing weight ratio	591:617	the increasing weight ratio of chitosan in the aerogels	591:645	The aerogel morphology observations showed that the pore diameter decreased with the increasing weight ratio of chitosan in the aerogels, but was even much lower after the crosslinking of excess alginate with calcium ions.
34044920	3	45	theme	alginate	705:712	arg1	crosslinking					682:693	the crosslinking	678:693	the crosslinking of excess alginate with calcium ions	678:730	The aerogel morphology observations showed that the pore diameter decreased with the increasing weight ratio of chitosan in the aerogels, but was even much lower after the crosslinking of excess alginate with calcium ions.
34044920	3	46	theme	weight	606:611	arg1	ratio					613:617	the increasing weight ratio	591:617	the increasing weight ratio of chitosan in the aerogels	591:645	The aerogel morphology observations showed that the pore diameter decreased with the increasing weight ratio of chitosan in the aerogels, but was even much lower after the crosslinking of excess alginate with calcium ions.
34044920	5	47	theme	physical	962:969	arg1	properties					989:998	the enhanced physical and antimicrobial properties	949:998	the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels	949:1032	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	3	48	from	ratio	613:617	arg1	aerogels					638:645	the aerogels	634:645	the aerogels	634:645	The aerogel morphology observations showed that the pore diameter decreased with the increasing weight ratio of chitosan in the aerogels, but was even much lower after the crosslinking of excess alginate with calcium ions.
34044920	4	49	theme	stronger	852:859	arg1	activities					875:884	stronger antibacterial activities	852:884	stronger antibacterial activities against Staphylococcus aureus and Escherichia coli	852:935	After crosslinking, the aerogels presented the improved thermal stability and higher mechanical properties, as well as stronger antibacterial activities against Staphylococcus aureus and Escherichia coli.
34044920	5	50	theme	enhanced	953:960	arg1	properties					989:998	the enhanced physical and antimicrobial properties	949:998	the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels	949:1032	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	0	51	theme	aerogels	78:85	arg1	properties					36:45	Enhanced physical and antimicrobial properties	0:45	Enhanced physical and antimicrobial properties of alginate/chitosan composite aerogels	0:85	Enhanced physical and antimicrobial properties of alginate/chitosan composite aerogels based on electrostatic interactions and noncovalent crosslinking.
34044920	5	52	theme	crosslinking	1110:1121	arg1	modulation					1053:1062	modulation	1053:1062	modulation of electrostatic interactions and noncovalent crosslinking	1053:1121	Therefore, the enhanced physical and antimicrobial properties of the alginate/chitosan aerogels may be achieved by modulation of electrostatic interactions and noncovalent crosslinking, suggesting the promising applications of these composite aerogels as active food packaging materials for antimicrobial purpose.
34044920	2	53	theme	alginate	421:428	arg1	solution					430:437	alginate solution	421:437	alginate solution	421:437	The solution property results showed that with the addition of chitosan in alginate solution, a tighter network was induced by the more entangled molecular chains.
34044920	1	54	theme	sol-gel	296:302	arg1	method					304:309	sol-gel method	296:309	sol-gel method followed by freeze-drying process	296:343	In this study, the alginate/chitosan composite aerogels based on electrostatic interactions and noncovalent crosslinking were fabricated using sol-gel method followed by freeze-drying process.
34582905	0	0	theme	Local	111:115	arg1	structure					117:125	Local structure	111:125	Local structure	111:125	Experimental and first-principle computational exploration on biomass cellulose/magnesium hydroxide composite: Local structure, interfacial interaction and antibacterial property.
34582905	2	1	theme	hydrogen	567:574	arg1	bonds					576:580	hydrogen bonds	567:580	hydrogen bonds involved in the vicinity of active sites and interfaces	567:636	However, it remains challenging due to the limitations of experimental techniques, particularly for the manner that biomass composites commonly have hydrogen bonds involved in the vicinity of active sites and interfaces.
34582905	3	2	theme	DFT	782:784	arg1	calculations					787:798	density functional theory (DFT) calculations	755:798	density functional theory (DFT) calculations	755:798	Herein, the cellulose/Mg(OH)2 nanocomposite has been synthesized via a simple hydrothermal approach and examined by density functional theory (DFT) calculations.
34582905	1	3	theme	tremendous	295:304	arg1	significance					306:317	tremendous significance	295:317	tremendous significance	295:317	The specification of the local structure and clarification of interfacial interactions of biomass composites is of tremendous significance in synthesizing novel materials and advancing their performance in various demanding applications.
34582905	3	4	theme	theory	774:779	arg1	calculations					787:798	density functional theory (DFT) calculations	755:798	density functional theory (DFT) calculations	755:798	Herein, the cellulose/Mg(OH)2 nanocomposite has been synthesized via a simple hydrothermal approach and examined by density functional theory (DFT) calculations.
34582905	5	5	theme	cellulose	934:942	arg1	surface					944:950	the cellulose surface	930:950	the cellulose surface	930:950	They either anchor onto the cellulose surface or intercalate between layers.
34582905	3	6	theme	density	755:761	arg1	calculations					787:798	density functional theory (DFT) calculations	755:798	density functional theory (DFT) calculations	755:798	Herein, the cellulose/Mg(OH)2 nanocomposite has been synthesized via a simple hydrothermal approach and examined by density functional theory (DFT) calculations.
34582905	4	7	theme	layered	826:832	arg1	morphology					834:843	a layered morphology	824:843	a layered morphology	824:843	The composite exhibits a layered morphology; Mg(OH)2 flakes are around 50 nm in size and well-dispersed.
34582905	1	8	from	performance	371:381	arg1	applications					404:415	various demanding applications	386:415	various demanding applications	386:415	The specification of the local structure and clarification of interfacial interactions of biomass composites is of tremendous significance in synthesizing novel materials and advancing their performance in various demanding applications.
34582905	2	9	theme	techniques	489:498	arg1	limitations					461:471	the limitations	457:471	the limitations of experimental techniques	457:498	However, it remains challenging due to the limitations of experimental techniques, particularly for the manner that biomass composites commonly have hydrogen bonds involved in the vicinity of active sites and interfaces.
34582905	1	10	theme	local	205:209	arg1	structure					211:219	local structure	205:219	local structure	205:219	The specification of the local structure and clarification of interfacial interactions of biomass composites is of tremendous significance in synthesizing novel materials and advancing their performance in various demanding applications.
34582905	0	11	theme	interfacial	128:138	arg1	interaction					140:150	interfacial interaction	128:150	interfacial interaction	128:150	Experimental and first-principle computational exploration on biomass cellulose/magnesium hydroxide composite: Local structure, interfacial interaction and antibacterial property.
34582905	7	12	theme	hydrogen	1135:1142	arg1	interactions					1105:1116	The interfacial interactions	1089:1116	The interfacial interactions	1089:1116	The interfacial interactions were found to be hydrogen bonding.
34582905	7	12	theme	hydrogen	1135:1142	arg1	bonding					1144:1150	hydrogen bonding	1135:1150	hydrogen bonding	1135:1150	The interfacial interactions were found to be hydrogen bonding.
34582905	1	13	theme	structure	211:219	arg1	specification					184:196	The specification	180:196	The specification of the local structure and clarification of interfacial interactions of biomass composites	180:287	The specification of the local structure and clarification of interfacial interactions of biomass composites is of tremendous significance in synthesizing novel materials and advancing their performance in various demanding applications.
34582905	3	14	theme	simple	710:715	arg1	approach					730:737	a simple hydrothermal approach	708:737	a simple hydrothermal approach	708:737	Herein, the cellulose/Mg(OH)2 nanocomposite has been synthesized via a simple hydrothermal approach and examined by density functional theory (DFT) calculations.
34582905	0	15	from	exploration	47:57	arg1	composite					100:108	biomass cellulose/magnesium hydroxide composite	62:108	biomass cellulose/magnesium hydroxide composite	62:108	Experimental and first-principle computational exploration on biomass cellulose/magnesium hydroxide composite: Local structure, interfacial interaction and antibacterial property.
34582905	9	16	theme	combined	1342:1349	arg1	results					1378:1384	The combined computational/experimental results	1338:1384	The combined computational/experimental results	1338:1384	The combined computational/experimental results allow to postulate the antibacterial mechanism of the nanocomposite.
34582905	8	17	dep	-0.47	1228:1232	arg1	cluster					1329:1335	2 cluster	1327:1335	2 cluster	1327:1335	The average adsorption energy per hydroxyl group was computed to be within -0.47 and -0.26 eV for a composite model comprising three cellulose chains and a two-layered Mg(OH)2 cluster.
34582905	8	18	theme	cellulose	1286:1294	arg1	chains					1296:1301	three cellulose chains	1280:1301	three cellulose chains	1280:1301	The average adsorption energy per hydroxyl group was computed to be within -0.47 and -0.26 eV for a composite model comprising three cellulose chains and a two-layered Mg(OH)2 cluster.
34582905	0	19	theme	Experimental	0:11	arg1	exploration					47:57	Experimental and first-principle computational exploration	0:57	Experimental and first-principle computational exploration on biomass cellulose/magnesium hydroxide composite: Local structure, interfacial interaction and antibacterial property.	0:178	Experimental and first-principle computational exploration on biomass cellulose/magnesium hydroxide composite: Local structure, interfacial interaction and antibacterial property.
34582905	1	20	theme	novel	335:339	arg1	materials					341:349	novel materials	335:349	novel materials	335:349	The specification of the local structure and clarification of interfacial interactions of biomass composites is of tremendous significance in synthesizing novel materials and advancing their performance in various demanding applications.
34582905	8	21	theme	hydroxyl	1187:1194	arg1	group					1196:1200	hydroxyl group	1187:1200	hydroxyl group	1187:1200	The average adsorption energy per hydroxyl group was computed to be within -0.47 and -0.26 eV for a composite model comprising three cellulose chains and a two-layered Mg(OH)2 cluster.
34582905	1	22	theme	clarification	225:237	arg1	specification					184:196	The specification	180:196	The specification of the local structure and clarification of interfacial interactions of biomass composites	180:287	The specification of the local structure and clarification of interfacial interactions of biomass composites is of tremendous significance in synthesizing novel materials and advancing their performance in various demanding applications.
34582905	4	23	from	50 nm	872:876	arg1	size					881:884	size	881:884	size	881:884	The composite exhibits a layered morphology; Mg(OH)2 flakes are around 50 nm in size and well-dispersed.
34582905	4	23	from	50 nm	872:876	arg1	well-dispersed					890:903	well-dispersed	890:903	well-dispersed	890:903	The composite exhibits a layered morphology; Mg(OH)2 flakes are around 50 nm in size and well-dispersed.
34582905	9	24	theme	computational/experimental	1351:1376	arg1	results					1378:1384	The combined computational/experimental results	1338:1384	The combined computational/experimental results	1338:1384	The combined computational/experimental results allow to postulate the antibacterial mechanism of the nanocomposite.
34582905	0	25	theme	antibacterial	156:168	arg1	property					170:177	antibacterial property	156:177	antibacterial property	156:177	Experimental and first-principle computational exploration on biomass cellulose/magnesium hydroxide composite: Local structure, interfacial interaction and antibacterial property.
34582905	1	26	dep	structure	211:219	arg1	the					201:203	the	201:203	the	201:203	The specification of the local structure and clarification of interfacial interactions of biomass composites is of tremendous significance in synthesizing novel materials and advancing their performance in various demanding applications.
34582905	6	27	theme	TEM	1071:1073	arg1	observations					1075:1086	XRD, SEM and TEM observations	1058:1086	XRD, SEM and TEM observations	1058:1086	The specific composite structure was confirmed theoretically, in line with XRD, SEM and TEM observations.
34582905	0	28	theme	first-principle	17:31	arg1	exploration					47:57	Experimental and first-principle computational exploration	0:57	Experimental and first-principle computational exploration on biomass cellulose/magnesium hydroxide composite: Local structure, interfacial interaction and antibacterial property.	0:178	Experimental and first-principle computational exploration on biomass cellulose/magnesium hydroxide composite: Local structure, interfacial interaction and antibacterial property.
34582905	2	29	theme	biomass	534:540	arg1	composites					542:551	biomass composites	534:551	biomass composites	534:551	However, it remains challenging due to the limitations of experimental techniques, particularly for the manner that biomass composites commonly have hydrogen bonds involved in the vicinity of active sites and interfaces.
34582905	1	30	theme	interfacial	242:252	arg1	interactions					254:265	interfacial interactions	242:265	interfacial interactions of biomass composites	242:287	The specification of the local structure and clarification of interfacial interactions of biomass composites is of tremendous significance in synthesizing novel materials and advancing their performance in various demanding applications.
34582905	6	31	theme	composite	996:1004	arg1	structure					1006:1014	The specific composite structure	983:1014	The specific composite structure	983:1014	The specific composite structure was confirmed theoretically, in line with XRD, SEM and TEM observations.
34582905	2	32	theme	sites	617:621	arg1	vicinity					598:605	the vicinity	594:605	the vicinity of active sites and interfaces	594:636	However, it remains challenging due to the limitations of experimental techniques, particularly for the manner that biomass composites commonly have hydrogen bonds involved in the vicinity of active sites and interfaces.
34582905	2	33	theme	experimental	476:487	arg1	techniques					489:498	experimental techniques	476:498	experimental techniques	476:498	However, it remains challenging due to the limitations of experimental techniques, particularly for the manner that biomass composites commonly have hydrogen bonds involved in the vicinity of active sites and interfaces.
34582905	3	34	theme	OH	664:665	arg1	nanocomposite					669:681	the cellulose/Mg(OH)2 nanocomposite	647:681	the cellulose/Mg(OH)2 nanocomposite	647:681	Herein, the cellulose/Mg(OH)2 nanocomposite has been synthesized via a simple hydrothermal approach and examined by density functional theory (DFT) calculations.
34582905	1	35	theme	interactions	254:265	arg1	clarification					225:237	clarification	225:237	clarification	225:237	The specification of the local structure and clarification of interfacial interactions of biomass composites is of tremendous significance in synthesizing novel materials and advancing their performance in various demanding applications.
34582905	1	35	theme	interactions	254:265	arg1	structure					211:219	local structure	205:219	local structure	205:219	The specification of the local structure and clarification of interfacial interactions of biomass composites is of tremendous significance in synthesizing novel materials and advancing their performance in various demanding applications.
34582905	6	36	theme	specific	987:994	arg1	structure					1006:1014	The specific composite structure	983:1014	The specific composite structure	983:1014	The specific composite structure was confirmed theoretically, in line with XRD, SEM and TEM observations.
34582905	2	37	theme	active	610:615	arg1	sites					617:621	active sites	610:621	active sites	610:621	However, it remains challenging due to the limitations of experimental techniques, particularly for the manner that biomass composites commonly have hydrogen bonds involved in the vicinity of active sites and interfaces.
34582905	8	38	theme	two-layered	1309:1319	arg1	OH					1324:1325	OH	1324:1325	OH	1324:1325	The average adsorption energy per hydroxyl group was computed to be within -0.47 and -0.26 eV for a composite model comprising three cellulose chains and a two-layered Mg(OH)2 cluster.
34582905	8	38	theme	two-layered	1309:1319	arg1	Mg					1321:1322	a two-layered Mg	1307:1322	a two-layered Mg(OH)	1307:1326	The average adsorption energy per hydroxyl group was computed to be within -0.47 and -0.26 eV for a composite model comprising three cellulose chains and a two-layered Mg(OH)2 cluster.
34582905	2	39	contain	have	562:565	arg2	manner					522:527	the manner	518:527	the manner that biomass composites commonly have hydrogen bonds involved in the vicinity of active sites and interfaces	518:636	However, it remains challenging due to the limitations of experimental techniques, particularly for the manner that biomass composites commonly have hydrogen bonds involved in the vicinity of active sites and interfaces.
34582905	2	39	contain	have	562:565	arg1	composites					542:551	biomass composites	534:551	biomass composites	534:551	However, it remains challenging due to the limitations of experimental techniques, particularly for the manner that biomass composites commonly have hydrogen bonds involved in the vicinity of active sites and interfaces.
34582905	2	39	contain	have	562:565	arg2	bonds					576:580	hydrogen bonds	567:580	hydrogen bonds involved in the vicinity of active sites and interfaces	567:636	However, it remains challenging due to the limitations of experimental techniques, particularly for the manner that biomass composites commonly have hydrogen bonds involved in the vicinity of active sites and interfaces.
34582905	9	40	theme	antibacterial	1409:1421	arg1	mechanism					1423:1431	the antibacterial mechanism	1405:1431	the antibacterial mechanism of the nanocomposite	1405:1452	The combined computational/experimental results allow to postulate the antibacterial mechanism of the nanocomposite.
34582905	0	41	theme	cellulose/magnesium	70:88	arg1	composite					100:108	biomass cellulose/magnesium hydroxide composite	62:108	biomass cellulose/magnesium hydroxide composite	62:108	Experimental and first-principle computational exploration on biomass cellulose/magnesium hydroxide composite: Local structure, interfacial interaction and antibacterial property.
34582905	8	42	theme	adsorption	1165:1174	arg1	energy					1176:1181	The average adsorption energy	1153:1181	The average adsorption energy per hydroxyl group	1153:1200	The average adsorption energy per hydroxyl group was computed to be within -0.47 and -0.26 eV for a composite model comprising three cellulose chains and a two-layered Mg(OH)2 cluster.
34582905	3	43	theme	cellulose/Mg	651:662	arg1	nanocomposite					669:681	the cellulose/Mg(OH)2 nanocomposite	647:681	the cellulose/Mg(OH)2 nanocomposite	647:681	Herein, the cellulose/Mg(OH)2 nanocomposite has been synthesized via a simple hydrothermal approach and examined by density functional theory (DFT) calculations.
34582905	7	44	theme	interfacial	1093:1103	arg1	interactions					1105:1116	The interfacial interactions	1089:1116	The interfacial interactions	1089:1116	The interfacial interactions were found to be hydrogen bonding.
34582905	7	44	theme	interfacial	1093:1103	arg1	bonding					1144:1150	hydrogen bonding	1135:1150	hydrogen bonding	1135:1150	The interfacial interactions were found to be hydrogen bonding.
34582905	0	45	theme	biomass	62:68	arg1	composite					100:108	biomass cellulose/magnesium hydroxide composite	62:108	biomass cellulose/magnesium hydroxide composite	62:108	Experimental and first-principle computational exploration on biomass cellulose/magnesium hydroxide composite: Local structure, interfacial interaction and antibacterial property.
34582905	2	46	theme	interfaces	627:636	arg1	vicinity					598:605	the vicinity	594:605	the vicinity of active sites and interfaces	594:636	However, it remains challenging due to the limitations of experimental techniques, particularly for the manner that biomass composites commonly have hydrogen bonds involved in the vicinity of active sites and interfaces.
34582905	0	47	dep	exploration	47:57	arg1	property					170:177	antibacterial property	156:177	antibacterial property	156:177	Experimental and first-principle computational exploration on biomass cellulose/magnesium hydroxide composite: Local structure, interfacial interaction and antibacterial property.
34582905	0	47	dep	exploration	47:57	arg1	structure					117:125	Local structure	111:125	Local structure	111:125	Experimental and first-principle computational exploration on biomass cellulose/magnesium hydroxide composite: Local structure, interfacial interaction and antibacterial property.
34582905	0	47	dep	exploration	47:57	arg1	interaction					140:150	interfacial interaction	128:150	interfacial interaction	128:150	Experimental and first-principle computational exploration on biomass cellulose/magnesium hydroxide composite: Local structure, interfacial interaction and antibacterial property.
34582905	3	48	theme	functional	763:772	arg1	calculations					787:798	density functional theory (DFT) calculations	755:798	density functional theory (DFT) calculations	755:798	Herein, the cellulose/Mg(OH)2 nanocomposite has been synthesized via a simple hydrothermal approach and examined by density functional theory (DFT) calculations.
34582905	1	49	theme	various	386:392	arg1	applications					404:415	various demanding applications	386:415	various demanding applications	386:415	The specification of the local structure and clarification of interfacial interactions of biomass composites is of tremendous significance in synthesizing novel materials and advancing their performance in various demanding applications.
34582905	8	50	theme	average	1157:1163	arg1	energy					1176:1181	The average adsorption energy	1153:1181	The average adsorption energy per hydroxyl group	1153:1200	The average adsorption energy per hydroxyl group was computed to be within -0.47 and -0.26 eV for a composite model comprising three cellulose chains and a two-layered Mg(OH)2 cluster.
34582905	1	51	theme	biomass	270:276	arg1	composites					278:287	biomass composites	270:287	biomass composites	270:287	The specification of the local structure and clarification of interfacial interactions of biomass composites is of tremendous significance in synthesizing novel materials and advancing their performance in various demanding applications.
34582905	3	52	theme	hydrothermal	717:728	arg1	approach					730:737	a simple hydrothermal approach	708:737	a simple hydrothermal approach	708:737	Herein, the cellulose/Mg(OH)2 nanocomposite has been synthesized via a simple hydrothermal approach and examined by density functional theory (DFT) calculations.
34582905	4	53	theme	Mg	846:847	arg1	flakes					854:859	Mg(OH)2 flakes	846:859	Mg(OH)2 flakes	846:859	The composite exhibits a layered morphology; Mg(OH)2 flakes are around 50 nm in size and well-dispersed.
34582905	8	54	theme	composite	1253:1261	arg1	model					1263:1267	a composite model	1251:1267	a composite model comprising three cellulose chains	1251:1301	The average adsorption energy per hydroxyl group was computed to be within -0.47 and -0.26 eV for a composite model comprising three cellulose chains and a two-layered Mg(OH)2 cluster.
34582905	1	55	theme	composites	278:287	arg1	interactions					254:265	interfacial interactions	242:265	interfacial interactions of biomass composites	242:287	The specification of the local structure and clarification of interfacial interactions of biomass composites is of tremendous significance in synthesizing novel materials and advancing their performance in various demanding applications.
34582905	6	56	with	line	1048:1051	arg1	observations					1075:1086	XRD, SEM and TEM observations	1058:1086	XRD, SEM and TEM observations	1058:1086	The specific composite structure was confirmed theoretically, in line with XRD, SEM and TEM observations.
34582905	6	57	theme	XRD	1058:1060	arg1	observations					1075:1086	XRD, SEM and TEM observations	1058:1086	XRD, SEM and TEM observations	1058:1086	The specific composite structure was confirmed theoretically, in line with XRD, SEM and TEM observations.
34582905	0	58	theme	hydroxide	90:98	arg1	composite					100:108	biomass cellulose/magnesium hydroxide composite	62:108	biomass cellulose/magnesium hydroxide composite	62:108	Experimental and first-principle computational exploration on biomass cellulose/magnesium hydroxide composite: Local structure, interfacial interaction and antibacterial property.
34582905	1	59	theme	demanding	394:402	arg1	applications					404:415	various demanding applications	386:415	various demanding applications	386:415	The specification of the local structure and clarification of interfacial interactions of biomass composites is of tremendous significance in synthesizing novel materials and advancing their performance in various demanding applications.
34582905	0	60	dep	Experimental	0:11	arg1	computational					33:45	computational	33:45	computational	33:45	Experimental and first-principle computational exploration on biomass cellulose/magnesium hydroxide composite: Local structure, interfacial interaction and antibacterial property.
34582905	6	61	theme	SEM	1063:1065	arg1	observations					1075:1086	XRD, SEM and TEM observations	1058:1086	XRD, SEM and TEM observations	1058:1086	The specific composite structure was confirmed theoretically, in line with XRD, SEM and TEM observations.
34582905	9	62	theme	nanocomposite	1440:1452	arg1	mechanism					1423:1431	the antibacterial mechanism	1405:1431	the antibacterial mechanism of the nanocomposite	1405:1452	The combined computational/experimental results allow to postulate the antibacterial mechanism of the nanocomposite.
34610375	3	0	contain	had	514:516	arg1	biocomposites					467:479	the biocomposites	463:479	the biocomposites made of smaller size wood powder	463:512	The biocomposites made of larger size wood powder had better tensile strength (40.3 MPa) and the biocomposites made of smaller size wood powder had the greater bending strength (50.5 MPa).
34610375	3	0	contain	had	514:516	arg2	50.5 MPa					548:555	50.5 MPa	548:555	50.5 MPa	548:555	The biocomposites made of larger size wood powder had better tensile strength (40.3 MPa) and the biocomposites made of smaller size wood powder had the greater bending strength (50.5 MPa).
34610375	3	0	contain	had	514:516	arg2	strength					538:545	the greater bending strength	518:545	the greater bending strength (50.5 MPa)	518:556	The biocomposites made of larger size wood powder had better tensile strength (40.3 MPa) and the biocomposites made of smaller size wood powder had the greater bending strength (50.5 MPa).
34610375	7	1	theme	traditional	1013:1023	arg1	boards					1033:1038	the traditional density boards	1009:1038	the traditional density boards made of adhesives	1009:1056	In addition, the biocomposites with excellent mechanical properties had no formaldehyde release, which can replace the traditional density boards made of adhesives and applied as furniture materials and in line with the concept of cleaner production.
34610375	1	2	theme	global	163:168	arg1	development					182:192	global sustainable development	163:192	global sustainable development	163:192	Green composite processing technology of wood fibers is an inevitable choice for global sustainable development.
34610375	5	3	dep	collapses	732:740	arg1	enhanced					742:749	enhanced	742:749	collapses enhanced the interfacial bonding	732:773	The cross-section morphology of the biocomposites showed that the cell wall collapses enhanced the interfacial bonding.
34610375	1	4	theme	sustainable	170:180	arg1	development					182:192	global sustainable development	163:192	global sustainable development	163:192	Green composite processing technology of wood fibers is an inevitable choice for global sustainable development.
34610375	5	5	theme	cell	722:725	arg1	wall					727:730	the cell wall	718:730	the cell wall	718:730	The cross-section morphology of the biocomposites showed that the cell wall collapses enhanced the interfacial bonding.
34610375	7	6	with	biocomposites	911:923	arg1	properties					951:960	excellent mechanical properties	930:960	excellent mechanical properties	930:960	In addition, the biocomposites with excellent mechanical properties had no formaldehyde release, which can replace the traditional density boards made of adhesives and applied as furniture materials and in line with the concept of cleaner production.
34610375	7	7	theme	production	1133:1142	arg1	concept					1114:1120	the concept	1110:1120	the concept of cleaner production	1110:1142	In addition, the biocomposites with excellent mechanical properties had no formaldehyde release, which can replace the traditional density boards made of adhesives and applied as furniture materials and in line with the concept of cleaner production.
34610375	3	8	contain	had	420:422	arg2	strength					439:446	better tensile strength	424:446	better tensile strength (40.3 MPa)	424:457	The biocomposites made of larger size wood powder had better tensile strength (40.3 MPa) and the biocomposites made of smaller size wood powder had the greater bending strength (50.5 MPa).
34610375	3	8	contain	had	420:422	arg1	biocomposites					374:386	The biocomposites	370:386	The biocomposites made of larger size wood powder	370:418	The biocomposites made of larger size wood powder had better tensile strength (40.3 MPa) and the biocomposites made of smaller size wood powder had the greater bending strength (50.5 MPa).
34610375	3	8	contain	had	420:422	arg2	40.3 MPa					449:456	40.3 MPa	449:456	40.3 MPa	449:456	The biocomposites made of larger size wood powder had better tensile strength (40.3 MPa) and the biocomposites made of smaller size wood powder had the greater bending strength (50.5 MPa).
34610375	2	9	theme	good	312:315	arg1	properties					343:352	good mechanical and waterproof properties	312:352	good mechanical and waterproof properties	312:352	In this research, waste poplar powder with different particle sizes was used to prepare glue-free biocomposites with good mechanical and waterproof properties by hot-molding.
34610375	4	10	theme	biocomposites	594:606	arg1	rate					582:585	The thickness swelling rate	559:585	The thickness swelling rate of the biocomposites	559:606	The thickness swelling rate of the biocomposites was only 4.26% after soaking in water for 24 h.
34610375	4	10	theme	biocomposites	594:606	arg1	%					621:621	only 4.26%	612:621	only 4.26%	612:621	The thickness swelling rate of the biocomposites was only 4.26% after soaking in water for 24 h.
34610375	2	11	theme	waterproof	332:341	arg1	properties					343:352	good mechanical and waterproof properties	312:352	good mechanical and waterproof properties	312:352	In this research, waste poplar powder with different particle sizes was used to prepare glue-free biocomposites with good mechanical and waterproof properties by hot-molding.
34610375	3	12	theme	size	497:500	arg1	powder					507:512	smaller size wood powder	489:512	smaller size wood powder	489:512	The biocomposites made of larger size wood powder had better tensile strength (40.3 MPa) and the biocomposites made of smaller size wood powder had the greater bending strength (50.5 MPa).
34610375	3	13	theme	wood	502:505	arg1	powder					507:512	smaller size wood powder	489:512	smaller size wood powder	489:512	The biocomposites made of larger size wood powder had better tensile strength (40.3 MPa) and the biocomposites made of smaller size wood powder had the greater bending strength (50.5 MPa).
34610375	1	14	theme	Green	82:86	arg1	technology					109:118	Green composite processing technology	82:118	Green composite processing technology of wood fibers	82:133	Green composite processing technology of wood fibers is an inevitable choice for global sustainable development.
34610375	1	14	theme	Green	82:86	arg1	choice					152:157	an inevitable choice	138:157	an inevitable choice for global sustainable development	138:192	Green composite processing technology of wood fibers is an inevitable choice for global sustainable development.
34610375	7	15	contain	had	962:964	arg1	biocomposites					911:923	the biocomposites	907:923	the biocomposites with excellent mechanical properties	907:960	In addition, the biocomposites with excellent mechanical properties had no formaldehyde release, which can replace the traditional density boards made of adhesives and applied as furniture materials and in line with the concept of cleaner production.
34610375	7	15	contain	had	962:964	arg2	release					982:988	no formaldehyde release	966:988	no formaldehyde release	966:988	In addition, the biocomposites with excellent mechanical properties had no formaldehyde release, which can replace the traditional density boards made of adhesives and applied as furniture materials and in line with the concept of cleaner production.
34610375	7	16	theme	formaldehyde	969:980	arg1	release					982:988	no formaldehyde release	966:988	no formaldehyde release	966:988	In addition, the biocomposites with excellent mechanical properties had no formaldehyde release, which can replace the traditional density boards made of adhesives and applied as furniture materials and in line with the concept of cleaner production.
34610375	1	17	theme	composite	88:96	arg1	technology					109:118	Green composite processing technology	82:118	Green composite processing technology of wood fibers	82:133	Green composite processing technology of wood fibers is an inevitable choice for global sustainable development.
34610375	1	17	theme	composite	88:96	arg1	choice					152:157	an inevitable choice	138:157	an inevitable choice for global sustainable development	138:192	Green composite processing technology of wood fibers is an inevitable choice for global sustainable development.
34610375	2	18	used	used	267:270	arg2	powder					226:231	waste poplar powder	213:231	waste poplar powder with different particle sizes	213:261	In this research, waste poplar powder with different particle sizes was used to prepare glue-free biocomposites with good mechanical and waterproof properties by hot-molding.
34610375	3	19	theme	wood	408:411	arg1	powder					413:418	larger size wood powder	396:418	larger size wood powder	396:418	The biocomposites made of larger size wood powder had better tensile strength (40.3 MPa) and the biocomposites made of smaller size wood powder had the greater bending strength (50.5 MPa).
34610375	1	20	theme	processing	98:107	arg1	technology					109:118	Green composite processing technology	82:118	Green composite processing technology of wood fibers	82:133	Green composite processing technology of wood fibers is an inevitable choice for global sustainable development.
34610375	1	20	theme	processing	98:107	arg1	choice					152:157	an inevitable choice	138:157	an inevitable choice for global sustainable development	138:192	Green composite processing technology of wood fibers is an inevitable choice for global sustainable development.
34610375	0	21	theme	Bio-based	0:8	arg1	composites					10:19	Bio-based composites	0:19	Bio-based composites	0:19	Bio-based composites fabricated from wood fibers through self-bonding technology.
34610375	3	22	theme	larger	396:401	arg1	powder					413:418	larger size wood powder	396:418	larger size wood powder	396:418	The biocomposites made of larger size wood powder had better tensile strength (40.3 MPa) and the biocomposites made of smaller size wood powder had the greater bending strength (50.5 MPa).
34610375	2	23	with	powder	226:231	arg1	sizes					257:261	different particle sizes	238:261	different particle sizes	238:261	In this research, waste poplar powder with different particle sizes was used to prepare glue-free biocomposites with good mechanical and waterproof properties by hot-molding.
34610375	5	24	theme	biocomposites	692:704	arg1	morphology					674:683	The cross-section morphology	656:683	The cross-section morphology of the biocomposites	656:704	The cross-section morphology of the biocomposites showed that the cell wall collapses enhanced the interfacial bonding.
34610375	6	25	theme	Chemical	776:783	arg1	analysis					785:792	Chemical analysis	776:792	Chemical analysis	776:792	Chemical analysis showed that lignin repolymerized with cellulose and hemicellulose for the vitrification transition.
34610375	2	26	theme	mechanical	317:326	arg1	properties					343:352	good mechanical and waterproof properties	312:352	good mechanical and waterproof properties	312:352	In this research, waste poplar powder with different particle sizes was used to prepare glue-free biocomposites with good mechanical and waterproof properties by hot-molding.
34610375	3	27	theme	size	403:406	arg1	powder					413:418	larger size wood powder	396:418	larger size wood powder	396:418	The biocomposites made of larger size wood powder had better tensile strength (40.3 MPa) and the biocomposites made of smaller size wood powder had the greater bending strength (50.5 MPa).
34610375	4	28	theme	swelling	573:580	arg1	rate					582:585	The thickness swelling rate	559:585	The thickness swelling rate of the biocomposites	559:606	The thickness swelling rate of the biocomposites was only 4.26% after soaking in water for 24 h.
34610375	4	28	theme	swelling	573:580	arg1	%					621:621	only 4.26%	612:621	only 4.26%	612:621	The thickness swelling rate of the biocomposites was only 4.26% after soaking in water for 24 h.
34610375	2	29	theme	glue-free	283:291	arg1	biocomposites					293:305	glue-free biocomposites	283:305	glue-free biocomposites with good mechanical and waterproof properties	283:352	In this research, waste poplar powder with different particle sizes was used to prepare glue-free biocomposites with good mechanical and waterproof properties by hot-molding.
34610375	1	30	theme	wood	123:126	arg1	fibers					128:133	wood fibers	123:133	wood fibers	123:133	Green composite processing technology of wood fibers is an inevitable choice for global sustainable development.
34610375	3	31	theme	tensile	431:437	arg1	40.3 MPa					449:456	40.3 MPa	449:456	40.3 MPa	449:456	The biocomposites made of larger size wood powder had better tensile strength (40.3 MPa) and the biocomposites made of smaller size wood powder had the greater bending strength (50.5 MPa).
34610375	3	31	theme	tensile	431:437	arg1	strength					439:446	better tensile strength	424:446	better tensile strength (40.3 MPa)	424:457	The biocomposites made of larger size wood powder had better tensile strength (40.3 MPa) and the biocomposites made of smaller size wood powder had the greater bending strength (50.5 MPa).
34610375	4	32	theme	thickness	563:571	arg1	rate					582:585	The thickness swelling rate	559:585	The thickness swelling rate of the biocomposites	559:606	The thickness swelling rate of the biocomposites was only 4.26% after soaking in water for 24 h.
34610375	4	32	theme	thickness	563:571	arg1	%					621:621	only 4.26%	612:621	only 4.26%	612:621	The thickness swelling rate of the biocomposites was only 4.26% after soaking in water for 24 h.
34610375	2	33	theme	particle	248:255	arg1	sizes					257:261	different particle sizes	238:261	different particle sizes	238:261	In this research, waste poplar powder with different particle sizes was used to prepare glue-free biocomposites with good mechanical and waterproof properties by hot-molding.
34610375	7	34	with	line	1100:1103	arg1	concept					1114:1120	the concept	1110:1120	the concept of cleaner production	1110:1142	In addition, the biocomposites with excellent mechanical properties had no formaldehyde release, which can replace the traditional density boards made of adhesives and applied as furniture materials and in line with the concept of cleaner production.
34610375	0	35	theme	wood	37:40	arg1	fibers					42:47	wood fibers	37:47	wood fibers	37:47	Bio-based composites fabricated from wood fibers through self-bonding technology.
34610375	2	36	theme	different	238:246	arg1	sizes					257:261	different particle sizes	238:261	different particle sizes	238:261	In this research, waste poplar powder with different particle sizes was used to prepare glue-free biocomposites with good mechanical and waterproof properties by hot-molding.
34610375	0	37	theme	self-bonding	57:68	arg1	technology					70:79	self-bonding technology	57:79	self-bonding technology	57:79	Bio-based composites fabricated from wood fibers through self-bonding technology.
34610375	5	38	theme	cross-section	660:672	arg1	morphology					674:683	The cross-section morphology	656:683	The cross-section morphology of the biocomposites	656:704	The cross-section morphology of the biocomposites showed that the cell wall collapses enhanced the interfacial bonding.
34610375	6	39	theme	vitrification	868:880	arg1	transition					882:891	the vitrification transition	864:891	the vitrification transition	864:891	Chemical analysis showed that lignin repolymerized with cellulose and hemicellulose for the vitrification transition.
34610375	1	40	theme	fibers	128:133	arg1	technology					109:118	Green composite processing technology	82:118	Green composite processing technology of wood fibers	82:133	Green composite processing technology of wood fibers is an inevitable choice for global sustainable development.
34610375	1	40	theme	fibers	128:133	arg1	choice					152:157	an inevitable choice	138:157	an inevitable choice for global sustainable development	138:192	Green composite processing technology of wood fibers is an inevitable choice for global sustainable development.
34610375	3	41	theme	better	424:429	arg1	40.3 MPa					449:456	40.3 MPa	449:456	40.3 MPa	449:456	The biocomposites made of larger size wood powder had better tensile strength (40.3 MPa) and the biocomposites made of smaller size wood powder had the greater bending strength (50.5 MPa).
34610375	3	41	theme	better	424:429	arg1	strength					439:446	better tensile strength	424:446	better tensile strength (40.3 MPa)	424:457	The biocomposites made of larger size wood powder had better tensile strength (40.3 MPa) and the biocomposites made of smaller size wood powder had the greater bending strength (50.5 MPa).
34610375	2	42	theme	poplar	219:224	arg1	powder					226:231	waste poplar powder	213:231	waste poplar powder with different particle sizes	213:261	In this research, waste poplar powder with different particle sizes was used to prepare glue-free biocomposites with good mechanical and waterproof properties by hot-molding.
34610375	7	43	theme	cleaner	1125:1131	arg1	production					1133:1142	cleaner production	1125:1142	cleaner production	1125:1142	In addition, the biocomposites with excellent mechanical properties had no formaldehyde release, which can replace the traditional density boards made of adhesives and applied as furniture materials and in line with the concept of cleaner production.
34610375	7	44	theme	mechanical	940:949	arg1	properties					951:960	excellent mechanical properties	930:960	excellent mechanical properties	930:960	In addition, the biocomposites with excellent mechanical properties had no formaldehyde release, which can replace the traditional density boards made of adhesives and applied as furniture materials and in line with the concept of cleaner production.
34610375	2	45	theme	waste	213:217	arg1	powder					226:231	waste poplar powder	213:231	waste poplar powder with different particle sizes	213:261	In this research, waste poplar powder with different particle sizes was used to prepare glue-free biocomposites with good mechanical and waterproof properties by hot-molding.
34610375	3	46	theme	smaller	489:495	arg1	powder					507:512	smaller size wood powder	489:512	smaller size wood powder	489:512	The biocomposites made of larger size wood powder had better tensile strength (40.3 MPa) and the biocomposites made of smaller size wood powder had the greater bending strength (50.5 MPa).
34610375	7	47	theme	density	1025:1031	arg1	boards					1033:1038	the traditional density boards	1009:1038	the traditional density boards made of adhesives	1009:1056	In addition, the biocomposites with excellent mechanical properties had no formaldehyde release, which can replace the traditional density boards made of adhesives and applied as furniture materials and in line with the concept of cleaner production.
34610375	1	48	theme	inevitable	141:150	arg1	technology					109:118	Green composite processing technology	82:118	Green composite processing technology of wood fibers	82:133	Green composite processing technology of wood fibers is an inevitable choice for global sustainable development.
34610375	1	48	theme	inevitable	141:150	arg1	choice					152:157	an inevitable choice	138:157	an inevitable choice for global sustainable development	138:192	Green composite processing technology of wood fibers is an inevitable choice for global sustainable development.
34610375	7	49	theme	furniture	1073:1081	arg1	materials					1083:1091	furniture materials	1073:1091	furniture materials	1073:1091	In addition, the biocomposites with excellent mechanical properties had no formaldehyde release, which can replace the traditional density boards made of adhesives and applied as furniture materials and in line with the concept of cleaner production.
34610375	3	50	theme	greater	522:528	arg1	50.5 MPa					548:555	50.5 MPa	548:555	50.5 MPa	548:555	The biocomposites made of larger size wood powder had better tensile strength (40.3 MPa) and the biocomposites made of smaller size wood powder had the greater bending strength (50.5 MPa).
34610375	3	50	theme	greater	522:528	arg1	strength					538:545	the greater bending strength	518:545	the greater bending strength (50.5 MPa)	518:556	The biocomposites made of larger size wood powder had better tensile strength (40.3 MPa) and the biocomposites made of smaller size wood powder had the greater bending strength (50.5 MPa).
34610375	7	51	theme	excellent	930:938	arg1	properties					951:960	excellent mechanical properties	930:960	excellent mechanical properties	930:960	In addition, the biocomposites with excellent mechanical properties had no formaldehyde release, which can replace the traditional density boards made of adhesives and applied as furniture materials and in line with the concept of cleaner production.
34610375	5	52	theme	interfacial	755:765	arg1	bonding					767:773	the interfacial bonding	751:773	the interfacial bonding	751:773	The cross-section morphology of the biocomposites showed that the cell wall collapses enhanced the interfacial bonding.
34610375	3	53	theme	bending	530:536	arg1	50.5 MPa					548:555	50.5 MPa	548:555	50.5 MPa	548:555	The biocomposites made of larger size wood powder had better tensile strength (40.3 MPa) and the biocomposites made of smaller size wood powder had the greater bending strength (50.5 MPa).
34610375	3	53	theme	bending	530:536	arg1	strength					538:545	the greater bending strength	518:545	the greater bending strength (50.5 MPa)	518:556	The biocomposites made of larger size wood powder had better tensile strength (40.3 MPa) and the biocomposites made of smaller size wood powder had the greater bending strength (50.5 MPa).
34610375	2	54	with	biocomposites	293:305	arg1	properties					343:352	good mechanical and waterproof properties	312:352	good mechanical and waterproof properties	312:352	In this research, waste poplar powder with different particle sizes was used to prepare glue-free biocomposites with good mechanical and waterproof properties by hot-molding.
32593919	7	0	theme	magnitude	1084:1092	arg1	orders					1074:1079	up to 5 orders	1066:1079	up to 5 orders of magnitude	1066:1092	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C had bacterial surface loads reduced up to 5 orders of magnitude compared to non-wrapped samples and had 4 orders of magnitude less bacterial surface loads than at the beginning of the experiments.
32593919	4	1	theme	Eucalyptus	504:513	arg1	extract					530:536	Eucalyptus globulus leafs extract	504:536	Eucalyptus globulus leafs extract (1%, 2%, 3%, 4%)	504:553	Nano-composite films were manufactured using Eucalyptus globulus leafs extract (1%, 2%, 3%, 4%).
32593919	6	2	theme	4	706:706	arg1	%					707:707	%	707:707	%	707:707	Nano-composite films with 4% Eucalyptus globulus leafs extract was best to provide better barrier, antioxidant properties to grapes when wrapped and stored at room temperature (25 ± 2)°C for 7 days and refrigerator temperature (4 ± 1)°C for 28 days.
32593919	3	3	theme	humidity	437:444	arg1	%					448:448	relative humidity 50%	428:448	relative humidity 50%	428:448	Starch nano-composite films were manufactured using varying concentration of CNF (5%, 10%, 15%, 20%) and mechanical properties of the films were studied at relative humidity 50% and 75%.
32593919	6	4	theme	antioxidant	779:789	arg1	barrier					770:776	better barrier	763:776	better barrier	763:776	Nano-composite films with 4% Eucalyptus globulus leafs extract was best to provide better barrier, antioxidant properties to grapes when wrapped and stored at room temperature (25 ± 2)°C for 7 days and refrigerator temperature (4 ± 1)°C for 28 days.
32593919	6	4	theme	antioxidant	779:789	arg1	properties					791:800	antioxidant properties	779:800	antioxidant properties to grapes	779:810	Nano-composite films with 4% Eucalyptus globulus leafs extract was best to provide better barrier, antioxidant properties to grapes when wrapped and stored at room temperature (25 ± 2)°C for 7 days and refrigerator temperature (4 ± 1)°C for 28 days.
32593919	4	5	theme	leafs	524:528	arg1	extract					530:536	Eucalyptus globulus leafs extract	504:536	Eucalyptus globulus leafs extract (1%, 2%, 3%, 4%)	504:553	Nano-composite films were manufactured using Eucalyptus globulus leafs extract (1%, 2%, 3%, 4%).
32593919	10	6	theme	4	1585:1585	arg1	%					1586:1586	%	1586:1586	%	1586:1586	The results showed that nano-composite with 4% Eucalyptus globulus leafs extract films have immense potential as food packaging/wrapping material.
32593919	10	7	theme	nano-composite	1565:1578	arg1	films					1622:1626	nano-composite with 4% Eucalyptus globulus leafs extract films	1565:1626	nano-composite with 4% Eucalyptus globulus leafs extract films	1565:1626	The results showed that nano-composite with 4% Eucalyptus globulus leafs extract films have immense potential as food packaging/wrapping material.
32593919	1	8	theme	carrot	174:179	arg1	pulp					181:184	carrot pulp	174:184	carrot pulp	174:184	Carrot cellulose nano fibers (CNF) have been extracted from carrot pulp using acid hydrolysis process.
32593919	7	9	dep	extract	979:985	arg1	either					1006:1011	either	1006:1011	either	1006:1011	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C had bacterial surface loads reduced up to 5 orders of magnitude compared to non-wrapped samples and had 4 orders of magnitude less bacterial surface loads than at the beginning of the experiments.
32593919	10	10	theme	leafs	1608:1612	arg1	extract					1614:1620	4% Eucalyptus globulus leafs extract	1585:1620	4% Eucalyptus globulus leafs extract	1585:1620	The results showed that nano-composite with 4% Eucalyptus globulus leafs extract films have immense potential as food packaging/wrapping material.
32593919	7	11	theme	Wrapped	930:936	arg1	grapes					938:943	Wrapped grapes	930:943	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C	930:1028	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C had bacterial surface loads reduced up to 5 orders of magnitude compared to non-wrapped samples and had 4 orders of magnitude less bacterial surface loads than at the beginning of the experiments.
32593919	10	12	theme	Eucalyptus	1588:1597	arg1	extract					1614:1620	4% Eucalyptus globulus leafs extract	1585:1620	4% Eucalyptus globulus leafs extract	1585:1620	The results showed that nano-composite with 4% Eucalyptus globulus leafs extract films have immense potential as food packaging/wrapping material.
32593919	1	13	theme	hydrolysis	197:206	arg1	process					208:214	acid hydrolysis process	192:214	acid hydrolysis process	192:214	Carrot cellulose nano fibers (CNF) have been extracted from carrot pulp using acid hydrolysis process.
32593919	4	14	dep	Eucalyptus	504:513	arg1	globulus					515:522	globulus	515:522	globulus	515:522	Nano-composite films were manufactured using Eucalyptus globulus leafs extract (1%, 2%, 3%, 4%).
32593919	10	15	theme	immense	1633:1639	arg1	potential					1641:1649	immense potential	1633:1649	immense potential	1633:1649	The results showed that nano-composite with 4% Eucalyptus globulus leafs extract films have immense potential as food packaging/wrapping material.
32593919	3	16	theme	Starch	272:277	arg1	films					294:298	Starch nano-composite films	272:298	Starch nano-composite films	272:298	Starch nano-composite films were manufactured using varying concentration of CNF (5%, 10%, 15%, 20%) and mechanical properties of the films were studied at relative humidity 50% and 75%.
32593919	8	17	theme	coli	1332:1335	arg1	growth					1310:1315	the growth	1306:1315	the growth of Escherichia coli, Listeria monocytogenes, Salmonella typhimurium and Penicillium spp	1306:1403	Wrapped grapes with NCC/Eucalyptus globulus leaf extract significantly reduced the growth of Escherichia coli, Listeria monocytogenes, Salmonella typhimurium and Penicillium spp.
32593919	0	18	dep	Eucalyptus	80:89	arg1	globulus					91:98	globulus	91:98	globulus	91:98	Synthesis and characterization of starch nanocellulosic films incorporated with Eucalyptus globulus leaf extract.
32593919	5	19	theme	Physio-chemical	556:570	arg1	properties					642:651	antioxidant, antimicrobial, morphological and crystalline properties	584:651	antioxidant, antimicrobial, morphological and crystalline properties of the films	584:664	Physio-chemical properties, antioxidant, antimicrobial, morphological and crystalline properties of the films were studied.
32593919	5	19	theme	Physio-chemical	556:570	arg1	properties					572:581	Physio-chemical properties	556:581	Physio-chemical properties	556:581	Physio-chemical properties, antioxidant, antimicrobial, morphological and crystalline properties of the films were studied.
32593919	10	20	theme	packaging/wrapping	1659:1676	arg1	material					1678:1685	food packaging/wrapping material	1654:1685	food packaging/wrapping material	1654:1685	The results showed that nano-composite with 4% Eucalyptus globulus leafs extract films have immense potential as food packaging/wrapping material.
32593919	6	21	theme	refrigerator	882:893	arg1	4 ± 1					908:912	4 ± 1	908:912	4 ± 1	908:912	Nano-composite films with 4% Eucalyptus globulus leafs extract was best to provide better barrier, antioxidant properties to grapes when wrapped and stored at room temperature (25 ± 2)°C for 7 days and refrigerator temperature (4 ± 1)°C for 28 days.
32593919	6	21	theme	refrigerator	882:893	arg1	temperature					895:905	refrigerator temperature	882:905	refrigerator temperature (4 ± 1)	882:913	Nano-composite films with 4% Eucalyptus globulus leafs extract was best to provide better barrier, antioxidant properties to grapes when wrapped and stored at room temperature (25 ± 2)°C for 7 days and refrigerator temperature (4 ± 1)°C for 28 days.
32593919	3	22	theme	varying	324:330	arg1	concentration					332:344	varying concentration	324:344	varying concentration of CNF (5%, 10%, 15%, 20%)	324:371	Starch nano-composite films were manufactured using varying concentration of CNF (5%, 10%, 15%, 20%) and mechanical properties of the films were studied at relative humidity 50% and 75%.
32593919	6	23	dep	temperature	844:854	arg1	°C					864:865	°C	864:865	room temperature (25 ± 2)°C for 7 days	839:876	Nano-composite films with 4% Eucalyptus globulus leafs extract was best to provide better barrier, antioxidant properties to grapes when wrapped and stored at room temperature (25 ± 2)°C for 7 days and refrigerator temperature (4 ± 1)°C for 28 days.
32593919	6	23	dep	temperature	844:854	arg1	25 ± 2					857:862	25 ± 2	857:862	25 ± 2	857:862	Nano-composite films with 4% Eucalyptus globulus leafs extract was best to provide better barrier, antioxidant properties to grapes when wrapped and stored at room temperature (25 ± 2)°C for 7 days and refrigerator temperature (4 ± 1)°C for 28 days.
32593919	6	23	dep	temperature	844:854	arg1	°C					914:915	°C	914:915	°C	914:915	Nano-composite films with 4% Eucalyptus globulus leafs extract was best to provide better barrier, antioxidant properties to grapes when wrapped and stored at room temperature (25 ± 2)°C for 7 days and refrigerator temperature (4 ± 1)°C for 28 days.
32593919	2	24	theme	CNF	233:235	arg1	size					221:224	The size	217:224	The size of the CNF	217:235	The size of the CNF was in the range of 6.33-58.77 nm.
32593919	8	25	dep	NCC/Eucalyptus	1247:1260	arg1	globulus					1262:1269	globulus	1262:1269	globulus	1262:1269	Wrapped grapes with NCC/Eucalyptus globulus leaf extract significantly reduced the growth of Escherichia coli, Listeria monocytogenes, Salmonella typhimurium and Penicillium spp.
32593919	8	26	theme	typhimurium	1373:1383	arg1	growth					1310:1315	the growth	1306:1315	the growth of Escherichia coli, Listeria monocytogenes, Salmonella typhimurium and Penicillium spp	1306:1403	Wrapped grapes with NCC/Eucalyptus globulus leaf extract significantly reduced the growth of Escherichia coli, Listeria monocytogenes, Salmonella typhimurium and Penicillium spp.
32593919	2	27	theme	6.33-58.77 nm	257:269	arg1	range					248:252	the range	244:252	the range of 6.33-58.77 nm	244:269	The size of the CNF was in the range of 6.33-58.77 nm.
32593919	0	28	theme	nanocellulosic	41:54	arg1	films					56:60	starch nanocellulosic films	34:60	starch nanocellulosic films	34:60	Synthesis and characterization of starch nanocellulosic films incorporated with Eucalyptus globulus leaf extract.
32593919	10	29	with	nano-composite	1565:1578	arg1	extract					1614:1620	4% Eucalyptus globulus leafs extract	1585:1620	4% Eucalyptus globulus leafs extract	1585:1620	The results showed that nano-composite with 4% Eucalyptus globulus leafs extract films have immense potential as food packaging/wrapping material.
32593919	0	30	theme	Eucalyptus	80:89	arg1	extract					105:111	Eucalyptus globulus leaf extract	80:111	Eucalyptus globulus leaf extract	80:111	Synthesis and characterization of starch nanocellulosic films incorporated with Eucalyptus globulus leaf extract.
32593919	7	31	theme	bacterial	1034:1042	arg1	loads					1052:1056	bacterial surface loads	1034:1056	bacterial surface loads	1034:1056	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C had bacterial surface loads reduced up to 5 orders of magnitude compared to non-wrapped samples and had 4 orders of magnitude less bacterial surface loads than at the beginning of the experiments.
32593919	8	32	theme	Listeria	1338:1345	arg1	monocytogenes					1347:1359	Listeria monocytogenes	1338:1359	Listeria monocytogenes	1338:1359	Wrapped grapes with NCC/Eucalyptus globulus leaf extract significantly reduced the growth of Escherichia coli, Listeria monocytogenes, Salmonella typhimurium and Penicillium spp.
32593919	7	33	theme	surface	1171:1177	arg1	loads					1179:1183	bacterial surface loads	1161:1183	bacterial surface loads	1161:1183	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C had bacterial surface loads reduced up to 5 orders of magnitude compared to non-wrapped samples and had 4 orders of magnitude less bacterial surface loads than at the beginning of the experiments.
32593919	4	34	theme	Nano-composite	459:472	arg1	films					474:478	Nano-composite films	459:478	Nano-composite films	459:478	Nano-composite films were manufactured using Eucalyptus globulus leafs extract (1%, 2%, 3%, 4%).
32593919	8	35	theme	Wrapped	1227:1233	arg1	grapes					1235:1240	Wrapped grapes	1227:1240	Wrapped grapes with NCC/Eucalyptus globulus leaf extract	1227:1282	Wrapped grapes with NCC/Eucalyptus globulus leaf extract significantly reduced the growth of Escherichia coli, Listeria monocytogenes, Salmonella typhimurium and Penicillium spp.
32593919	1	36	theme	cellulose	121:129	arg1	CNF					144:146	CNF	144:146	CNF	144:146	Carrot cellulose nano fibers (CNF) have been extracted from carrot pulp using acid hydrolysis process.
32593919	1	36	theme	cellulose	121:129	arg1	fibers					136:141	Carrot cellulose nano fibers	114:141	Carrot cellulose nano fibers (CNF)	114:147	Carrot cellulose nano fibers (CNF) have been extracted from carrot pulp using acid hydrolysis process.
32593919	7	37	theme	less	1156:1159	arg1	orders					1136:1141	4 orders	1134:1141	4 orders of magnitude less bacterial surface loads than at the beginning of the experiments	1134:1224	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C had bacterial surface loads reduced up to 5 orders of magnitude compared to non-wrapped samples and had 4 orders of magnitude less bacterial surface loads than at the beginning of the experiments.
32593919	3	38	theme	mechanical	377:386	arg1	properties					388:397	mechanical properties	377:397	mechanical properties of the films	377:410	Starch nano-composite films were manufactured using varying concentration of CNF (5%, 10%, 15%, 20%) and mechanical properties of the films were studied at relative humidity 50% and 75%.
32593919	0	39	theme	starch	34:39	arg1	films					56:60	starch nanocellulosic films	34:60	starch nanocellulosic films	34:60	Synthesis and characterization of starch nanocellulosic films incorporated with Eucalyptus globulus leaf extract.
32593919	0	40	theme	films	56:60	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of starch nanocellulosic films incorporated with Eucalyptus globulus leaf extract.
32593919	0	40	theme	films	56:60	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of starch nanocellulosic films incorporated with Eucalyptus globulus leaf extract.
32593919	6	41	theme	leafs	729:733	arg1	extract					735:741	4% Eucalyptus globulus leafs extract	706:741	4% Eucalyptus globulus leafs extract	706:741	Nano-composite films with 4% Eucalyptus globulus leafs extract was best to provide better barrier, antioxidant properties to grapes when wrapped and stored at room temperature (25 ± 2)°C for 7 days and refrigerator temperature (4 ± 1)°C for 28 days.
32593919	3	42	theme	films	406:410	arg1	properties					388:397	mechanical properties	377:397	mechanical properties of the films	377:410	Starch nano-composite films were manufactured using varying concentration of CNF (5%, 10%, 15%, 20%) and mechanical properties of the films were studied at relative humidity 50% and 75%.
32593919	6	43	theme	Eucalyptus	709:718	arg1	extract					735:741	4% Eucalyptus globulus leafs extract	706:741	4% Eucalyptus globulus leafs extract	706:741	Nano-composite films with 4% Eucalyptus globulus leafs extract was best to provide better barrier, antioxidant properties to grapes when wrapped and stored at room temperature (25 ± 2)°C for 7 days and refrigerator temperature (4 ± 1)°C for 28 days.
32593919	9	44	dep	°C	1511:1512	arg1	25 ± 2					1503:1508	25 ± 2	1503:1508	25 ± 2	1503:1508	when the cells of these organisms were spiked onto grapes and the samples were stored either at (25 ± 2) °C or (4 ± 1) °C for 28 days.
32593919	7	45	dep	less	1156:1159	arg1	loads					1179:1183	bacterial surface loads	1161:1183	bacterial surface loads	1161:1183	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C had bacterial surface loads reduced up to 5 orders of magnitude compared to non-wrapped samples and had 4 orders of magnitude less bacterial surface loads than at the beginning of the experiments.
32593919	7	46	theme	non-wrapped	1106:1116	arg1	samples					1118:1124	non-wrapped samples	1106:1124	non-wrapped samples	1106:1124	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C had bacterial surface loads reduced up to 5 orders of magnitude compared to non-wrapped samples and had 4 orders of magnitude less bacterial surface loads than at the beginning of the experiments.
32593919	9	47	dep	°C	1525:1526	arg1	4 ± 1					1518:1522	4 ± 1	1518:1522	4 ± 1	1518:1522	when the cells of these organisms were spiked onto grapes and the samples were stored either at (25 ± 2) °C or (4 ± 1) °C for 28 days.
32593919	3	48	theme	relative	428:435	arg1	%					448:448	relative humidity 50%	428:448	relative humidity 50%	428:448	Starch nano-composite films were manufactured using varying concentration of CNF (5%, 10%, 15%, 20%) and mechanical properties of the films were studied at relative humidity 50% and 75%.
32593919	7	49	theme	leaf	974:977	arg1	extract					979:985	NCC/Eucalyptus globulus leaf extract	950:985	NCC/Eucalyptus globulus leaf extract stored for 28 days	950:1004	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C had bacterial surface loads reduced up to 5 orders of magnitude compared to non-wrapped samples and had 4 orders of magnitude less bacterial surface loads than at the beginning of the experiments.
32593919	10	50	theme	%	1586:1586	arg1	extract					1614:1620	4% Eucalyptus globulus leafs extract	1585:1620	4% Eucalyptus globulus leafs extract	1585:1620	The results showed that nano-composite with 4% Eucalyptus globulus leafs extract films have immense potential as food packaging/wrapping material.
32593919	7	51	theme	NCC/Eucalyptus	950:963	arg1	extract					979:985	NCC/Eucalyptus globulus leaf extract	950:985	NCC/Eucalyptus globulus leaf extract stored for 28 days	950:1004	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C had bacterial surface loads reduced up to 5 orders of magnitude compared to non-wrapped samples and had 4 orders of magnitude less bacterial surface loads than at the beginning of the experiments.
32593919	6	52	theme	Nano-composite	680:693	arg1	films					695:699	Nano-composite films	680:699	Nano-composite films with 4% Eucalyptus globulus leafs extract	680:741	Nano-composite films with 4% Eucalyptus globulus leafs extract was best to provide better barrier, antioxidant properties to grapes when wrapped and stored at room temperature (25 ± 2)°C for 7 days and refrigerator temperature (4 ± 1)°C for 28 days.
32593919	6	53	theme	better	763:768	arg1	barrier					770:776	better barrier	763:776	better barrier	763:776	Nano-composite films with 4% Eucalyptus globulus leafs extract was best to provide better barrier, antioxidant properties to grapes when wrapped and stored at room temperature (25 ± 2)°C for 7 days and refrigerator temperature (4 ± 1)°C for 28 days.
32593919	6	53	theme	better	763:768	arg1	properties					791:800	antioxidant properties	779:800	antioxidant properties to grapes	779:810	Nano-composite films with 4% Eucalyptus globulus leafs extract was best to provide better barrier, antioxidant properties to grapes when wrapped and stored at room temperature (25 ± 2)°C for 7 days and refrigerator temperature (4 ± 1)°C for 28 days.
32593919	6	54	with	films	695:699	arg1	extract					735:741	4% Eucalyptus globulus leafs extract	706:741	4% Eucalyptus globulus leafs extract	706:741	Nano-composite films with 4% Eucalyptus globulus leafs extract was best to provide better barrier, antioxidant properties to grapes when wrapped and stored at room temperature (25 ± 2)°C for 7 days and refrigerator temperature (4 ± 1)°C for 28 days.
32593919	10	55	theme	globulus	1599:1606	arg1	extract					1614:1620	4% Eucalyptus globulus leafs extract	1585:1620	4% Eucalyptus globulus leafs extract	1585:1620	The results showed that nano-composite with 4% Eucalyptus globulus leafs extract films have immense potential as food packaging/wrapping material.
32593919	1	56	theme	acid	192:195	arg1	process					208:214	acid hydrolysis process	192:214	acid hydrolysis process	192:214	Carrot cellulose nano fibers (CNF) have been extracted from carrot pulp using acid hydrolysis process.
32593919	6	57	theme	room	839:842	arg1	temperature					844:854	room temperature (25 ± 2)°C for 7 days	839:876	room temperature (25 ± 2)°C for 7 days	839:876	Nano-composite films with 4% Eucalyptus globulus leafs extract was best to provide better barrier, antioxidant properties to grapes when wrapped and stored at room temperature (25 ± 2)°C for 7 days and refrigerator temperature (4 ± 1)°C for 28 days.
32593919	8	58	with	grapes	1235:1240	arg1	extract					1276:1282	NCC/Eucalyptus globulus leaf extract	1247:1282	NCC/Eucalyptus globulus leaf extract	1247:1282	Wrapped grapes with NCC/Eucalyptus globulus leaf extract significantly reduced the growth of Escherichia coli, Listeria monocytogenes, Salmonella typhimurium and Penicillium spp.
32593919	5	59	theme	antioxidant	584:594	arg1	properties					642:651	antioxidant, antimicrobial, morphological and crystalline properties	584:651	antioxidant, antimicrobial, morphological and crystalline properties of the films	584:664	Physio-chemical properties, antioxidant, antimicrobial, morphological and crystalline properties of the films were studied.
32593919	5	59	theme	antioxidant	584:594	arg1	properties					572:581	Physio-chemical properties	556:581	Physio-chemical properties	556:581	Physio-chemical properties, antioxidant, antimicrobial, morphological and crystalline properties of the films were studied.
32593919	7	60	dep	NCC/Eucalyptus	950:963	arg1	globulus					965:972	globulus	965:972	globulus	965:972	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C had bacterial surface loads reduced up to 5 orders of magnitude compared to non-wrapped samples and had 4 orders of magnitude less bacterial surface loads than at the beginning of the experiments.
32593919	1	61	theme	nano	131:134	arg1	CNF					144:146	CNF	144:146	CNF	144:146	Carrot cellulose nano fibers (CNF) have been extracted from carrot pulp using acid hydrolysis process.
32593919	1	61	theme	nano	131:134	arg1	fibers					136:141	Carrot cellulose nano fibers	114:141	Carrot cellulose nano fibers (CNF)	114:147	Carrot cellulose nano fibers (CNF) have been extracted from carrot pulp using acid hydrolysis process.
32593919	7	62	theme	experiments	1214:1224	arg1	beginning					1197:1205	the beginning	1193:1205	the beginning of the experiments	1193:1224	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C had bacterial surface loads reduced up to 5 orders of magnitude compared to non-wrapped samples and had 4 orders of magnitude less bacterial surface loads than at the beginning of the experiments.
32593919	3	63	theme	nano-composite	279:292	arg1	films					294:298	Starch nano-composite films	272:298	Starch nano-composite films	272:298	Starch nano-composite films were manufactured using varying concentration of CNF (5%, 10%, 15%, 20%) and mechanical properties of the films were studied at relative humidity 50% and 75%.
32593919	8	64	theme	monocytogenes	1347:1359	arg1	growth					1310:1315	the growth	1306:1315	the growth of Escherichia coli, Listeria monocytogenes, Salmonella typhimurium and Penicillium spp	1306:1403	Wrapped grapes with NCC/Eucalyptus globulus leaf extract significantly reduced the growth of Escherichia coli, Listeria monocytogenes, Salmonella typhimurium and Penicillium spp.
32593919	5	65	theme	crystalline	630:640	arg1	properties					642:651	antioxidant, antimicrobial, morphological and crystalline properties	584:651	antioxidant, antimicrobial, morphological and crystalline properties of the films	584:664	Physio-chemical properties, antioxidant, antimicrobial, morphological and crystalline properties of the films were studied.
32593919	5	65	theme	crystalline	630:640	arg1	properties					572:581	Physio-chemical properties	556:581	Physio-chemical properties	556:581	Physio-chemical properties, antioxidant, antimicrobial, morphological and crystalline properties of the films were studied.
32593919	4	66	dep	extract	530:536	arg1	%					544:544	2%	543:544	2%	543:544	Nano-composite films were manufactured using Eucalyptus globulus leafs extract (1%, 2%, 3%, 4%).
32593919	4	66	dep	extract	530:536	arg1	%					548:548	3%	547:548	3%	547:548	Nano-composite films were manufactured using Eucalyptus globulus leafs extract (1%, 2%, 3%, 4%).
32593919	4	66	dep	extract	530:536	arg1	%					552:552	4%	551:552	4%	551:552	Nano-composite films were manufactured using Eucalyptus globulus leafs extract (1%, 2%, 3%, 4%).
32593919	4	66	dep	extract	530:536	arg1	%					540:540	1%	539:540	1%	539:540	Nano-composite films were manufactured using Eucalyptus globulus leafs extract (1%, 2%, 3%, 4%).
32593919	10	67	theme	food	1654:1657	arg1	material					1678:1685	food packaging/wrapping material	1654:1685	food packaging/wrapping material	1654:1685	The results showed that nano-composite with 4% Eucalyptus globulus leafs extract films have immense potential as food packaging/wrapping material.
32593919	0	68	theme	leaf	100:103	arg1	extract					105:111	Eucalyptus globulus leaf extract	80:111	Eucalyptus globulus leaf extract	80:111	Synthesis and characterization of starch nanocellulosic films incorporated with Eucalyptus globulus leaf extract.
32593919	5	69	theme	antimicrobial	597:609	arg1	properties					642:651	antioxidant, antimicrobial, morphological and crystalline properties	584:651	antioxidant, antimicrobial, morphological and crystalline properties of the films	584:664	Physio-chemical properties, antioxidant, antimicrobial, morphological and crystalline properties of the films were studied.
32593919	5	69	theme	antimicrobial	597:609	arg1	properties					572:581	Physio-chemical properties	556:581	Physio-chemical properties	556:581	Physio-chemical properties, antioxidant, antimicrobial, morphological and crystalline properties of the films were studied.
32593919	8	70	theme	spp	1401:1403	arg1	growth					1310:1315	the growth	1306:1315	the growth of Escherichia coli, Listeria monocytogenes, Salmonella typhimurium and Penicillium spp	1306:1403	Wrapped grapes with NCC/Eucalyptus globulus leaf extract significantly reduced the growth of Escherichia coli, Listeria monocytogenes, Salmonella typhimurium and Penicillium spp.
32593919	5	71	theme	morphological	612:624	arg1	properties					642:651	antioxidant, antimicrobial, morphological and crystalline properties	584:651	antioxidant, antimicrobial, morphological and crystalline properties of the films	584:664	Physio-chemical properties, antioxidant, antimicrobial, morphological and crystalline properties of the films were studied.
32593919	5	71	theme	morphological	612:624	arg1	properties					572:581	Physio-chemical properties	556:581	Physio-chemical properties	556:581	Physio-chemical properties, antioxidant, antimicrobial, morphological and crystalline properties of the films were studied.
32593919	3	72	theme	CNF	349:351	arg1	concentration					332:344	varying concentration	324:344	varying concentration of CNF (5%, 10%, 15%, 20%)	324:371	Starch nano-composite films were manufactured using varying concentration of CNF (5%, 10%, 15%, 20%) and mechanical properties of the films were studied at relative humidity 50% and 75%.
32593919	7	73	theme	surface	1044:1050	arg1	loads					1052:1056	bacterial surface loads	1034:1056	bacterial surface loads	1034:1056	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C had bacterial surface loads reduced up to 5 orders of magnitude compared to non-wrapped samples and had 4 orders of magnitude less bacterial surface loads than at the beginning of the experiments.
32593919	7	74	contain	had	1130:1132	arg2	orders					1136:1141	4 orders	1134:1141	4 orders of magnitude less bacterial surface loads than at the beginning of the experiments	1134:1224	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C had bacterial surface loads reduced up to 5 orders of magnitude compared to non-wrapped samples and had 4 orders of magnitude less bacterial surface loads than at the beginning of the experiments.
32593919	7	74	contain	had	1130:1132	arg1	grapes					938:943	Wrapped grapes	930:943	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C	930:1028	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C had bacterial surface loads reduced up to 5 orders of magnitude compared to non-wrapped samples and had 4 orders of magnitude less bacterial surface loads than at the beginning of the experiments.
32593919	9	75	theme	organisms	1430:1438	arg1	cells					1415:1419	the cells	1411:1419	the cells of these organisms	1411:1438	when the cells of these organisms were spiked onto grapes and the samples were stored either at (25 ± 2) °C or (4 ± 1) °C for 28 days.
32593919	7	76	theme	bacterial	1161:1169	arg1	loads					1179:1183	bacterial surface loads	1161:1183	bacterial surface loads	1161:1183	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C had bacterial surface loads reduced up to 5 orders of magnitude compared to non-wrapped samples and had 4 orders of magnitude less bacterial surface loads than at the beginning of the experiments.
32593919	10	77	contain	have	1628:1631	arg1	films					1622:1626	nano-composite with 4% Eucalyptus globulus leafs extract films	1565:1626	nano-composite with 4% Eucalyptus globulus leafs extract films	1565:1626	The results showed that nano-composite with 4% Eucalyptus globulus leafs extract films have immense potential as food packaging/wrapping material.
32593919	10	77	contain	have	1628:1631	arg2	potential					1641:1649	immense potential	1633:1649	immense potential	1633:1649	The results showed that nano-composite with 4% Eucalyptus globulus leafs extract films have immense potential as food packaging/wrapping material.
32593919	1	78	theme	Carrot	114:119	arg1	CNF					144:146	CNF	144:146	CNF	144:146	Carrot cellulose nano fibers (CNF) have been extracted from carrot pulp using acid hydrolysis process.
32593919	1	78	theme	Carrot	114:119	arg1	fibers					136:141	Carrot cellulose nano fibers	114:141	Carrot cellulose nano fibers (CNF)	114:147	Carrot cellulose nano fibers (CNF) have been extracted from carrot pulp using acid hydrolysis process.
32593919	7	79	theme	magnitude	1146:1154	arg1	orders					1136:1141	4 orders	1134:1141	4 orders of magnitude less bacterial surface loads than at the beginning of the experiments	1134:1224	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C had bacterial surface loads reduced up to 5 orders of magnitude compared to non-wrapped samples and had 4 orders of magnitude less bacterial surface loads than at the beginning of the experiments.
32593919	7	80	dep	5	1072:1072	arg1	to					1069:1070	to	1069:1070	to	1069:1070	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C had bacterial surface loads reduced up to 5 orders of magnitude compared to non-wrapped samples and had 4 orders of magnitude less bacterial surface loads than at the beginning of the experiments.
32593919	5	81	theme	films	660:664	arg1	properties					642:651	antioxidant, antimicrobial, morphological and crystalline properties	584:651	antioxidant, antimicrobial, morphological and crystalline properties of the films	584:664	Physio-chemical properties, antioxidant, antimicrobial, morphological and crystalline properties of the films were studied.
32593919	5	81	theme	films	660:664	arg1	properties					572:581	Physio-chemical properties	556:581	Physio-chemical properties	556:581	Physio-chemical properties, antioxidant, antimicrobial, morphological and crystalline properties of the films were studied.
32593919	3	82	dep	concentration	332:344	arg1	%					355:355	5%	354:355	5%	354:355	Starch nano-composite films were manufactured using varying concentration of CNF (5%, 10%, 15%, 20%) and mechanical properties of the films were studied at relative humidity 50% and 75%.
32593919	3	82	dep	concentration	332:344	arg1	%					360:360	10%	358:360	10%	358:360	Starch nano-composite films were manufactured using varying concentration of CNF (5%, 10%, 15%, 20%) and mechanical properties of the films were studied at relative humidity 50% and 75%.
32593919	3	82	dep	concentration	332:344	arg1	%					365:365	15%	363:365	15%	363:365	Starch nano-composite films were manufactured using varying concentration of CNF (5%, 10%, 15%, 20%) and mechanical properties of the films were studied at relative humidity 50% and 75%.
32593919	3	82	dep	concentration	332:344	arg1	%					370:370	20%	368:370	20%	368:370	Starch nano-composite films were manufactured using varying concentration of CNF (5%, 10%, 15%, 20%) and mechanical properties of the films were studied at relative humidity 50% and 75%.
32593919	7	83	with	grapes	938:943	arg1	extract					979:985	NCC/Eucalyptus globulus leaf extract	950:985	NCC/Eucalyptus globulus leaf extract stored for 28 days	950:1004	Wrapped grapes with NCC/Eucalyptus globulus leaf extract stored for 28 days either at 25 °C or 4 °C had bacterial surface loads reduced up to 5 orders of magnitude compared to non-wrapped samples and had 4 orders of magnitude less bacterial surface loads than at the beginning of the experiments.
32593919	8	84	theme	NCC/Eucalyptus	1247:1260	arg1	extract					1276:1282	NCC/Eucalyptus globulus leaf extract	1247:1282	NCC/Eucalyptus globulus leaf extract	1247:1282	Wrapped grapes with NCC/Eucalyptus globulus leaf extract significantly reduced the growth of Escherichia coli, Listeria monocytogenes, Salmonella typhimurium and Penicillium spp.
32593919	6	85	theme	globulus	720:727	arg1	extract					735:741	4% Eucalyptus globulus leafs extract	706:741	4% Eucalyptus globulus leafs extract	706:741	Nano-composite films with 4% Eucalyptus globulus leafs extract was best to provide better barrier, antioxidant properties to grapes when wrapped and stored at room temperature (25 ± 2)°C for 7 days and refrigerator temperature (4 ± 1)°C for 28 days.
32593919	8	86	theme	leaf	1271:1274	arg1	extract					1276:1282	NCC/Eucalyptus globulus leaf extract	1247:1282	NCC/Eucalyptus globulus leaf extract	1247:1282	Wrapped grapes with NCC/Eucalyptus globulus leaf extract significantly reduced the growth of Escherichia coli, Listeria monocytogenes, Salmonella typhimurium and Penicillium spp.
32593919	6	87	theme	%	707:707	arg1	extract					735:741	4% Eucalyptus globulus leafs extract	706:741	4% Eucalyptus globulus leafs extract	706:741	Nano-composite films with 4% Eucalyptus globulus leafs extract was best to provide better barrier, antioxidant properties to grapes when wrapped and stored at room temperature (25 ± 2)°C for 7 days and refrigerator temperature (4 ± 1)°C for 28 days.
32707284	6	0	from	fluids	1357:1362	arg1	stability					1285:1293	increased stability	1275:1293	increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking	1275:1447	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	0	1	theme	methylesterification	147:166	arg1	degrees					136:142	low and high degrees	123:142	low and high degrees of methylesterification	123:166	Physicochemical and swelling properties of composite gel microparticles based on alginate and callus cultures pectins with low and high degrees of methylesterification.
32707284	3	2	theme	microrelief	687:697	arg1	increase					604:611	an increase	601:611	an increase in the microparticle gel strength	601:645	The inclusion of increasing concentrations of alginate in gel formulations promoted an increase in the microparticle gel strength and the formation of a smoother surface microrelief independently of the pectin chemical composition.
32707284	3	2	theme	microrelief	687:697	arg1	formation					655:663	the formation	651:663	the formation of a smoother surface microrelief independently of the pectin chemical composition	651:746	The inclusion of increasing concentrations of alginate in gel formulations promoted an increase in the microparticle gel strength and the formation of a smoother surface microrelief independently of the pectin chemical composition.
32707284	4	3	theme	swelling	893:900	arg1	degree					902:907	an increased swelling degree	880:907	an increased swelling degree in the simulated digestive fluids	880:941	Microparticles based on the pectin with a low degree of methylesterification (DM) and a higher concentration of alginate exhibited an increased swelling degree in the simulated digestive fluids.
32707284	3	4	theme	composition	736:746	arg1	microrelief					687:697	a smoother surface microrelief	668:697	a smoother surface microrelief independently of the pectin chemical composition	668:746	The inclusion of increasing concentrations of alginate in gel formulations promoted an increase in the microparticle gel strength and the formation of a smoother surface microrelief independently of the pectin chemical composition.
32707284	3	5	theme	concentrations	545:558	arg1	inclusion					521:529	The inclusion	517:529	The inclusion of increasing concentrations of alginate in gel formulations	517:590	The inclusion of increasing concentrations of alginate in gel formulations promoted an increase in the microparticle gel strength and the formation of a smoother surface microrelief independently of the pectin chemical composition.
32707284	1	6	theme	methylesterification	272:291	arg1	degrees					261:267	low and high degrees	248:267	low and high degrees of methylesterification or apple pectin	248:307	Composite gel microparticles based on alginate and callus culture pectins with low and high degrees of methylesterification or apple pectin were produced.
32707284	4	7	theme	concentration	844:856	arg1	degree					795:800	a low degree	789:800	a low degree of methylesterification (DM) and a higher concentration of alginate	789:868	Microparticles based on the pectin with a low degree of methylesterification (DM) and a higher concentration of alginate exhibited an increased swelling degree in the simulated digestive fluids.
32707284	3	8	from	formation	655:663	arg1	strength					638:645	the microparticle gel strength	616:645	the microparticle gel strength	616:645	The inclusion of increasing concentrations of alginate in gel formulations promoted an increase in the microparticle gel strength and the formation of a smoother surface microrelief independently of the pectin chemical composition.
32707284	3	9	theme	pectin	720:725	arg1	composition					736:746	the pectin chemical composition	716:746	the pectin chemical composition	716:746	The inclusion of increasing concentrations of alginate in gel formulations promoted an increase in the microparticle gel strength and the formation of a smoother surface microrelief independently of the pectin chemical composition.
32707284	4	10	theme	alginate	861:868	arg1	methylesterification					805:824	methylesterification	805:824	methylesterification (DM)	805:829	Microparticles based on the pectin with a low degree of methylesterification (DM) and a higher concentration of alginate exhibited an increased swelling degree in the simulated digestive fluids.
32707284	4	10	theme	alginate	861:868	arg1	concentration					844:856	a higher concentration	835:856	a higher concentration of alginate	835:868	Microparticles based on the pectin with a low degree of methylesterification (DM) and a higher concentration of alginate exhibited an increased swelling degree in the simulated digestive fluids.
32707284	4	10	theme	alginate	861:868	arg1	DM					827:828	DM	827:828	DM	827:828	Microparticles based on the pectin with a low degree of methylesterification (DM) and a higher concentration of alginate exhibited an increased swelling degree in the simulated digestive fluids.
32707284	5	11	with	pectin	972:977	arg1	concentration					1009:1021	low alginate concentration	996:1021	low alginate concentration	996:1021	Microparticles based on the pectin with high DM and low alginate concentration were destroyed in the simulated intestinal fluid within 1 h due to the low Ca2+ content, gel strength, and grooved and rough surface of these microparticles.
32707284	5	11	with	pectin	972:977	arg1	DM					989:990	high DM	984:990	high DM	984:990	Microparticles based on the pectin with high DM and low alginate concentration were destroyed in the simulated intestinal fluid within 1 h due to the low Ca2+ content, gel strength, and grooved and rough surface of these microparticles.
32707284	3	12	theme	gel	575:577	arg1	formulations					579:590	gel formulations	575:590	gel formulations	575:590	The inclusion of increasing concentrations of alginate in gel formulations promoted an increase in the microparticle gel strength and the formation of a smoother surface microrelief independently of the pectin chemical composition.
32707284	6	13	theme	simulated	1324:1332	arg1	fluids					1357:1362	the simulated intestinal and colonic fluids	1320:1362	the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking	1320:1447	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	3	14	theme	alginate	563:570	arg1	concentrations					545:558	increasing concentrations	534:558	increasing concentrations of alginate	534:570	The inclusion of increasing concentrations of alginate in gel formulations promoted an increase in the microparticle gel strength and the formation of a smoother surface microrelief independently of the pectin chemical composition.
32707284	0	15	theme	high	131:134	arg1	degrees					136:142	low and high degrees	123:142	low and high degrees of methylesterification	123:166	Physicochemical and swelling properties of composite gel microparticles based on alginate and callus cultures pectins with low and high degrees of methylesterification.
32707284	1	16	theme	culture	227:233	arg1	pectins					235:241	alginate and callus culture pectins	207:241	pectins	235:241	Composite gel microparticles based on alginate and callus culture pectins with low and high degrees of methylesterification or apple pectin were produced.
32707284	5	17	theme	microparticles	1165:1178	arg1	surface					1148:1154	grooved and rough surface	1130:1154	grooved and rough surface	1130:1154	Microparticles based on the pectin with high DM and low alginate concentration were destroyed in the simulated intestinal fluid within 1 h due to the low Ca2+ content, gel strength, and grooved and rough surface of these microparticles.
32707284	5	17	theme	microparticles	1165:1178	arg1	content					1103:1109	the low Ca2+ content	1090:1109	the low Ca2+ content	1090:1109	Microparticles based on the pectin with high DM and low alginate concentration were destroyed in the simulated intestinal fluid within 1 h due to the low Ca2+ content, gel strength, and grooved and rough surface of these microparticles.
32707284	5	17	theme	microparticles	1165:1178	arg1	strength					1116:1123	gel strength	1112:1123	gel strength	1112:1123	Microparticles based on the pectin with high DM and low alginate concentration were destroyed in the simulated intestinal fluid within 1 h due to the low Ca2+ content, gel strength, and grooved and rough surface of these microparticles.
32707284	6	18	theme	gel	1222:1224	arg1	formulations					1226:1237	gel formulations	1222:1237	gel formulations based on pectin with high DM	1222:1266	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	5	19	theme	intestinal	1055:1064	arg1	fluid					1066:1070	the simulated intestinal fluid	1041:1070	the simulated intestinal fluid within 1 h due to the low Ca2+ content, gel strength, and grooved and rough surface of these microparticles	1041:1178	Microparticles based on the pectin with high DM and low alginate concentration were destroyed in the simulated intestinal fluid within 1 h due to the low Ca2+ content, gel strength, and grooved and rough surface of these microparticles.
32707284	4	20	theme	simulated	916:924	arg1	fluids					936:941	the simulated digestive fluids	912:941	the simulated digestive fluids	912:941	Microparticles based on the pectin with a low degree of methylesterification (DM) and a higher concentration of alginate exhibited an increased swelling degree in the simulated digestive fluids.
32707284	3	21	theme	gel	634:636	arg1	strength					638:645	the microparticle gel strength	616:645	the microparticle gel strength	616:645	The inclusion of increasing concentrations of alginate in gel formulations promoted an increase in the microparticle gel strength and the formation of a smoother surface microrelief independently of the pectin chemical composition.
32707284	6	22	theme	Ca2+	1381:1384	arg1	content					1386:1392	increased Ca2+ content	1371:1392	increased Ca2+ content	1371:1392	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	2	23	theme	composite	476:484	arg1	microparticles					486:499	composite microparticles	476:499	composite microparticles	476:499	By varying the chemical composition of the pectic samples and the ratio of alginate to pectin, the gel strength, morphology, and swelling properties of composite microparticles can be altered.
32707284	6	24	from	increase	1184:1191	arg1	concentration					1205:1217	alginate concentration	1196:1217	alginate concentration of gel formulations based on pectin with high DM	1196:1266	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	0	25	theme	alginate	81:88	arg1	pectins					110:116	alginate and callus cultures pectins	81:116	alginate and callus cultures pectins with low and high degrees of methylesterification	81:166	Physicochemical and swelling properties of composite gel microparticles based on alginate and callus cultures pectins with low and high degrees of methylesterification.
32707284	1	26	theme	Composite	169:177	arg1	microparticles					183:196	Composite gel microparticles	169:196	Composite gel microparticles based on alginate and callus culture pectins with low and high degrees of methylesterification or apple pectin	169:307	Composite gel microparticles based on alginate and callus culture pectins with low and high degrees of methylesterification or apple pectin were produced.
32707284	0	27	theme	callus	94:99	arg1	cultures					101:108	callus cultures	94:108	callus cultures	94:108	Physicochemical and swelling properties of composite gel microparticles based on alginate and callus cultures pectins with low and high degrees of methylesterification.
32707284	5	28	theme	Ca2+	1098:1101	arg1	content					1103:1109	the low Ca2+ content	1090:1109	the low Ca2+ content	1090:1109	Microparticles based on the pectin with high DM and low alginate concentration were destroyed in the simulated intestinal fluid within 1 h due to the low Ca2+ content, gel strength, and grooved and rough surface of these microparticles.
32707284	5	29	theme	high	984:987	arg1	DM					989:990	high DM	984:990	high DM	984:990	Microparticles based on the pectin with high DM and low alginate concentration were destroyed in the simulated intestinal fluid within 1 h due to the low Ca2+ content, gel strength, and grooved and rough surface of these microparticles.
32707284	4	30	theme	low	791:793	arg1	degree					795:800	a low degree	789:800	a low degree of methylesterification (DM) and a higher concentration of alginate	789:868	Microparticles based on the pectin with a low degree of methylesterification (DM) and a higher concentration of alginate exhibited an increased swelling degree in the simulated digestive fluids.
32707284	2	31	theme	alginate	399:406	arg1	composition					348:358	the chemical composition	335:358	the chemical composition of the pectic samples	335:380	By varying the chemical composition of the pectic samples and the ratio of alginate to pectin, the gel strength, morphology, and swelling properties of composite microparticles can be altered.
32707284	2	31	theme	alginate	399:406	arg1	ratio					390:394	the ratio	386:394	the ratio of alginate to pectin	386:416	By varying the chemical composition of the pectic samples and the ratio of alginate to pectin, the gel strength, morphology, and swelling properties of composite microparticles can be altered.
32707284	2	32	theme	morphology	437:446	arg1	properties					462:471	the gel strength, morphology, and swelling properties	419:471	the gel strength, morphology, and swelling properties of composite microparticles	419:499	By varying the chemical composition of the pectic samples and the ratio of alginate to pectin, the gel strength, morphology, and swelling properties of composite microparticles can be altered.
32707284	0	33	theme	Physicochemical	0:14	arg1	properties					29:38	Physicochemical and swelling properties	0:38	Physicochemical and swelling properties of composite gel microparticles	0:70	Physicochemical and swelling properties of composite gel microparticles based on alginate and callus cultures pectins with low and high degrees of methylesterification.
32707284	2	34	theme	strength	427:434	arg1	properties					462:471	the gel strength, morphology, and swelling properties	419:471	the gel strength, morphology, and swelling properties of composite microparticles	419:499	By varying the chemical composition of the pectic samples and the ratio of alginate to pectin, the gel strength, morphology, and swelling properties of composite microparticles can be altered.
32707284	0	35	theme	swelling	20:27	arg1	properties					29:38	Physicochemical and swelling properties	0:38	Physicochemical and swelling properties of composite gel microparticles	0:70	Physicochemical and swelling properties of composite gel microparticles based on alginate and callus cultures pectins with low and high degrees of methylesterification.
32707284	1	36	theme	pectin	302:307	arg1	degrees					261:267	low and high degrees	248:267	low and high degrees of methylesterification or apple pectin	248:307	Composite gel microparticles based on alginate and callus culture pectins with low and high degrees of methylesterification or apple pectin were produced.
32707284	3	37	from	increase	604:611	arg1	strength					638:645	the microparticle gel strength	616:645	the microparticle gel strength	616:645	The inclusion of increasing concentrations of alginate in gel formulations promoted an increase in the microparticle gel strength and the formation of a smoother surface microrelief independently of the pectin chemical composition.
32707284	6	38	theme	gel	1409:1411	arg1	strength					1413:1420	microparticle gel strength	1395:1420	microparticle gel strength	1395:1420	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	0	39	theme	gel	53:55	arg1	microparticles					57:70	composite gel microparticles	43:70	composite gel microparticles	43:70	Physicochemical and swelling properties of composite gel microparticles based on alginate and callus cultures pectins with low and high degrees of methylesterification.
32707284	6	40	from	microparticles	1302:1315	arg1	fluids					1357:1362	the simulated intestinal and colonic fluids	1320:1362	the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking	1320:1447	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	5	41	theme	simulated	1045:1053	arg1	fluid					1066:1070	the simulated intestinal fluid	1041:1070	the simulated intestinal fluid within 1 h due to the low Ca2+ content, gel strength, and grooved and rough surface of these microparticles	1041:1178	Microparticles based on the pectin with high DM and low alginate concentration were destroyed in the simulated intestinal fluid within 1 h due to the low Ca2+ content, gel strength, and grooved and rough surface of these microparticles.
32707284	3	42	theme	surface	679:685	arg1	microrelief					687:697	a smoother surface microrelief	668:697	a smoother surface microrelief independently of the pectin chemical composition	668:746	The inclusion of increasing concentrations of alginate in gel formulations promoted an increase in the microparticle gel strength and the formation of a smoother surface microrelief independently of the pectin chemical composition.
32707284	2	43	theme	chemical	339:346	arg1	composition					348:358	the chemical composition	335:358	the chemical composition of the pectic samples	335:380	By varying the chemical composition of the pectic samples and the ratio of alginate to pectin, the gel strength, morphology, and swelling properties of composite microparticles can be altered.
32707284	5	44	theme	alginate	1000:1007	arg1	concentration					1009:1021	low alginate concentration	996:1021	low alginate concentration	996:1021	Microparticles based on the pectin with high DM and low alginate concentration were destroyed in the simulated intestinal fluid within 1 h due to the low Ca2+ content, gel strength, and grooved and rough surface of these microparticles.
32707284	1	45	theme	low	248:250	arg1	degrees					261:267	low and high degrees	248:267	low and high degrees of methylesterification or apple pectin	248:307	Composite gel microparticles based on alginate and callus culture pectins with low and high degrees of methylesterification or apple pectin were produced.
32707284	0	46	theme	microparticles	57:70	arg1	properties					29:38	Physicochemical and swelling properties	0:38	Physicochemical and swelling properties of composite gel microparticles	0:70	Physicochemical and swelling properties of composite gel microparticles based on alginate and callus cultures pectins with low and high degrees of methylesterification.
32707284	1	47	with	pectins	235:241	arg1	degrees					261:267	low and high degrees	248:267	low and high degrees of methylesterification or apple pectin	248:307	Composite gel microparticles based on alginate and callus culture pectins with low and high degrees of methylesterification or apple pectin were produced.
32707284	6	48	from	stability	1285:1293	arg1	fluids					1357:1362	the simulated intestinal and colonic fluids	1320:1362	the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking	1320:1447	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	6	49	theme	increased	1275:1283	arg1	stability					1285:1293	increased stability	1275:1293	increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking	1275:1447	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	2	50	theme	pectic	367:372	arg1	samples					374:380	the pectic samples	363:380	the pectic samples	363:380	By varying the chemical composition of the pectic samples and the ratio of alginate to pectin, the gel strength, morphology, and swelling properties of composite microparticles can be altered.
32707284	1	51	theme	high	256:259	arg1	degrees					261:267	low and high degrees	248:267	low and high degrees of methylesterification or apple pectin	248:307	Composite gel microparticles based on alginate and callus culture pectins with low and high degrees of methylesterification or apple pectin were produced.
32707284	3	52	theme	increasing	534:543	arg1	concentrations					545:558	increasing concentrations	534:558	increasing concentrations of alginate	534:570	The inclusion of increasing concentrations of alginate in gel formulations promoted an increase in the microparticle gel strength and the formation of a smoother surface microrelief independently of the pectin chemical composition.
32707284	4	53	theme	increased	883:891	arg1	degree					902:907	an increased swelling degree	880:907	an increased swelling degree in the simulated digestive fluids	880:941	Microparticles based on the pectin with a low degree of methylesterification (DM) and a higher concentration of alginate exhibited an increased swelling degree in the simulated digestive fluids.
32707284	6	54	theme	high	1260:1263	arg1	DM					1265:1266	high DM	1260:1266	high DM	1260:1266	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	1	55	theme	gel	179:181	arg1	microparticles					183:196	Composite gel microparticles	169:196	Composite gel microparticles based on alginate and callus culture pectins with low and high degrees of methylesterification or apple pectin	169:307	Composite gel microparticles based on alginate and callus culture pectins with low and high degrees of methylesterification or apple pectin were produced.
32707284	4	56	theme	higher	837:842	arg1	concentration					844:856	a higher concentration	835:856	a higher concentration of alginate	835:868	Microparticles based on the pectin with a low degree of methylesterification (DM) and a higher concentration of alginate exhibited an increased swelling degree in the simulated digestive fluids.
32707284	3	57	theme	chemical	727:734	arg1	composition					736:746	the pectin chemical composition	716:746	the pectin chemical composition	716:746	The inclusion of increasing concentrations of alginate in gel formulations promoted an increase in the microparticle gel strength and the formation of a smoother surface microrelief independently of the pectin chemical composition.
32707284	0	58	with	pectins	110:116	arg1	degrees					136:142	low and high degrees	123:142	low and high degrees of methylesterification	123:166	Physicochemical and swelling properties of composite gel microparticles based on alginate and callus cultures pectins with low and high degrees of methylesterification.
32707284	6	59	theme	intestinal	1334:1343	arg1	fluids					1357:1362	the simulated intestinal and colonic fluids	1320:1362	the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking	1320:1447	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	1	60	theme	alginate	207:214	arg1	pectins					235:241	alginate and callus culture pectins	207:241	pectins	235:241	Composite gel microparticles based on alginate and callus culture pectins with low and high degrees of methylesterification or apple pectin were produced.
32707284	4	61	theme	digestive	926:934	arg1	fluids					936:941	the simulated digestive fluids	912:941	the simulated digestive fluids	912:941	Microparticles based on the pectin with a low degree of methylesterification (DM) and a higher concentration of alginate exhibited an increased swelling degree in the simulated digestive fluids.
32707284	4	62	with	pectin	777:782	arg1	degree					795:800	a low degree	789:800	a low degree of methylesterification (DM) and a higher concentration of alginate	789:868	Microparticles based on the pectin with a low degree of methylesterification (DM) and a higher concentration of alginate exhibited an increased swelling degree in the simulated digestive fluids.
32707284	1	63	theme	callus	220:225	arg1	pectins					235:241	alginate and callus culture pectins	207:241	pectins	235:241	Composite gel microparticles based on alginate and callus culture pectins with low and high degrees of methylesterification or apple pectin were produced.
32707284	6	64	theme	formulations	1226:1237	arg1	concentration					1205:1217	alginate concentration	1196:1217	alginate concentration of gel formulations based on pectin with high DM	1196:1266	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	6	65	theme	due	1364:1366	arg1	fluids					1357:1362	the simulated intestinal and colonic fluids	1320:1362	the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking	1320:1447	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	6	66	theme	microparticles	1302:1315	arg1	stability					1285:1293	increased stability	1275:1293	increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking	1275:1447	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	5	67	theme	low	1094:1096	arg1	content					1103:1109	the low Ca2+ content	1090:1109	the low Ca2+ content	1090:1109	Microparticles based on the pectin with high DM and low alginate concentration were destroyed in the simulated intestinal fluid within 1 h due to the low Ca2+ content, gel strength, and grooved and rough surface of these microparticles.
32707284	3	68	theme	microparticle	620:632	arg1	strength					638:645	the microparticle gel strength	616:645	the microparticle gel strength	616:645	The inclusion of increasing concentrations of alginate in gel formulations promoted an increase in the microparticle gel strength and the formation of a smoother surface microrelief independently of the pectin chemical composition.
32707284	6	69	theme	alginate	1196:1203	arg1	concentration					1205:1217	alginate concentration	1196:1217	alginate concentration of gel formulations based on pectin with high DM	1196:1266	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	4	70	from	degree	902:907	arg1	fluids					936:941	the simulated digestive fluids	912:941	the simulated digestive fluids	912:941	Microparticles based on the pectin with a low degree of methylesterification (DM) and a higher concentration of alginate exhibited an increased swelling degree in the simulated digestive fluids.
32707284	2	71	theme	microparticles	486:499	arg1	properties					462:471	the gel strength, morphology, and swelling properties	419:471	the gel strength, morphology, and swelling properties of composite microparticles	419:499	By varying the chemical composition of the pectic samples and the ratio of alginate to pectin, the gel strength, morphology, and swelling properties of composite microparticles can be altered.
32707284	3	72	from	inclusion	521:529	arg1	formulations					579:590	gel formulations	575:590	gel formulations	575:590	The inclusion of increasing concentrations of alginate in gel formulations promoted an increase in the microparticle gel strength and the formation of a smoother surface microrelief independently of the pectin chemical composition.
32707284	6	73	theme	increased	1371:1379	arg1	content					1386:1392	increased Ca2+ content	1371:1392	increased Ca2+ content	1371:1392	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	0	74	theme	low	123:125	arg1	degrees					136:142	low and high degrees	123:142	low and high degrees of methylesterification	123:166	Physicochemical and swelling properties of composite gel microparticles based on alginate and callus cultures pectins with low and high degrees of methylesterification.
32707284	0	75	theme	cultures	101:108	arg1	pectins					110:116	alginate and callus cultures pectins	81:116	alginate and callus cultures pectins with low and high degrees of methylesterification	81:166	Physicochemical and swelling properties of composite gel microparticles based on alginate and callus cultures pectins with low and high degrees of methylesterification.
32707284	5	76	theme	grooved	1130:1136	arg1	surface					1148:1154	grooved and rough surface	1130:1154	grooved and rough surface	1130:1154	Microparticles based on the pectin with high DM and low alginate concentration were destroyed in the simulated intestinal fluid within 1 h due to the low Ca2+ content, gel strength, and grooved and rough surface of these microparticles.
32707284	6	77	theme	colonic	1349:1355	arg1	fluids					1357:1362	the simulated intestinal and colonic fluids	1320:1362	the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking	1320:1447	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	6	78	theme	crosslinking	1436:1447	arg1	degree					1426:1431	degree	1426:1431	degree of crosslinking	1426:1447	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	6	78	theme	crosslinking	1436:1447	arg1	strength					1413:1420	microparticle gel strength	1395:1420	microparticle gel strength	1395:1420	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	6	78	theme	crosslinking	1436:1447	arg1	content					1386:1392	increased Ca2+ content	1371:1392	increased Ca2+ content	1371:1392	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	5	79	theme	gel	1112:1114	arg1	strength					1116:1123	gel strength	1112:1123	gel strength	1112:1123	Microparticles based on the pectin with high DM and low alginate concentration were destroyed in the simulated intestinal fluid within 1 h due to the low Ca2+ content, gel strength, and grooved and rough surface of these microparticles.
32707284	1	80	theme	apple	296:300	arg1	pectin					302:307	apple pectin	296:307	apple pectin	296:307	Composite gel microparticles based on alginate and callus culture pectins with low and high degrees of methylesterification or apple pectin were produced.
32707284	2	81	theme	gel	423:425	arg1	strength					427:434	gel strength	423:434	gel strength	423:434	By varying the chemical composition of the pectic samples and the ratio of alginate to pectin, the gel strength, morphology, and swelling properties of composite microparticles can be altered.
32707284	0	82	theme	composite	43:51	arg1	microparticles					57:70	composite gel microparticles	43:70	composite gel microparticles	43:70	Physicochemical and swelling properties of composite gel microparticles based on alginate and callus cultures pectins with low and high degrees of methylesterification.
32707284	2	83	theme	swelling	453:460	arg1	properties					462:471	the gel strength, morphology, and swelling properties	419:471	the gel strength, morphology, and swelling properties of composite microparticles	419:499	By varying the chemical composition of the pectic samples and the ratio of alginate to pectin, the gel strength, morphology, and swelling properties of composite microparticles can be altered.
32707284	6	84	theme	microparticle	1395:1407	arg1	strength					1413:1420	microparticle gel strength	1395:1420	microparticle gel strength	1395:1420	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
32707284	5	85	theme	rough	1142:1146	arg1	surface					1148:1154	grooved and rough surface	1130:1154	grooved and rough surface	1130:1154	Microparticles based on the pectin with high DM and low alginate concentration were destroyed in the simulated intestinal fluid within 1 h due to the low Ca2+ content, gel strength, and grooved and rough surface of these microparticles.
32707284	3	86	theme	smoother	670:677	arg1	microrelief					687:697	a smoother surface microrelief	668:697	a smoother surface microrelief independently of the pectin chemical composition	668:746	The inclusion of increasing concentrations of alginate in gel formulations promoted an increase in the microparticle gel strength and the formation of a smoother surface microrelief independently of the pectin chemical composition.
32707284	5	87	theme	low	996:998	arg1	concentration					1009:1021	low alginate concentration	996:1021	low alginate concentration	996:1021	Microparticles based on the pectin with high DM and low alginate concentration were destroyed in the simulated intestinal fluid within 1 h due to the low Ca2+ content, gel strength, and grooved and rough surface of these microparticles.
32707284	4	88	theme	methylesterification	805:824	arg1	degree					795:800	a low degree	789:800	a low degree of methylesterification (DM) and a higher concentration of alginate	789:868	Microparticles based on the pectin with a low degree of methylesterification (DM) and a higher concentration of alginate exhibited an increased swelling degree in the simulated digestive fluids.
32707284	2	89	theme	samples	374:380	arg1	composition					348:358	the chemical composition	335:358	the chemical composition of the pectic samples	335:380	By varying the chemical composition of the pectic samples and the ratio of alginate to pectin, the gel strength, morphology, and swelling properties of composite microparticles can be altered.
32707284	2	89	theme	samples	374:380	arg1	ratio					390:394	the ratio	386:394	the ratio of alginate to pectin	386:416	By varying the chemical composition of the pectic samples and the ratio of alginate to pectin, the gel strength, morphology, and swelling properties of composite microparticles can be altered.
32707284	6	90	with	pectin	1248:1253	arg1	DM					1265:1266	high DM	1260:1266	high DM	1260:1266	An increase in alginate concentration of gel formulations based on pectin with high DM led to increased stability of the microparticles in the simulated intestinal and colonic fluids due to increased Ca2+ content, microparticle gel strength and degree of crosslinking.
33488263	7	0	theme	EndoBI	1105:1110	arg1	enzymes					1112:1118	EndoBI enzymes	1105:1118	EndoBI enzymes	1105:1118	Selected models were used for docking studies of the plus subsite ligand of these enzymes for further understanding on the substrate selectivity of EndoBI enzymes.
33488263	5	1	from	sequences	710:718	arg1	databases					733:741	different databases	723:741	different databases	723:741	In this study, the growing numbers of homologous sequences in different databases were exploited in a comparative approach to investigate structural properties of EndoBI-1 and EndoBI-2 enzymes.
33488263	5	2	from	databases	733:741	arg1	numbers					688:694	the growing numbers	676:694	the growing numbers of homologous sequences in different databases	676:741	In this study, the growing numbers of homologous sequences in different databases were exploited in a comparative approach to investigate structural properties of EndoBI-1 and EndoBI-2 enzymes.
33488263	5	3	theme	structural	799:808	arg1	properties					810:819	structural properties	799:819	structural properties of EndoBI-1 and EndoBI-2 enzymes	799:852	In this study, the growing numbers of homologous sequences in different databases were exploited in a comparative approach to investigate structural properties of EndoBI-1 and EndoBI-2 enzymes.
33488263	1	4	theme	N-N'-	179:183	arg1	moieties					205:212	N-N'- diacetylchitobiosyl moieties	179:212	N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans	179:256	EndoBI-1 and EndoBI-2 are two endo- β-N- acetylglucosaminidase isoenzymes that cleave N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans.
33488263	2	5	theme	hydrolases	361:370	arg1	lack					332:335	the lack	328:335	the lack of responsible glycosyl hydrolases	328:370	These N -glycans are indigestible by human infants and adults due to the lack of responsible glycosyl hydrolases and they act as selective prebiotics for a probiotic microorganism, Bifidobacterium longum subsp .
33488263	7	6	theme	plus	1010:1013	arg1	ligand					1023:1028	the plus subsite ligand	1006:1028	the plus subsite ligand of these enzymes for further understanding on the substrate selectivity of EndoBI enzymes	1006:1118	Selected models were used for docking studies of the plus subsite ligand of these enzymes for further understanding on the substrate selectivity of EndoBI enzymes.
33488263	5	7	theme	growing	680:686	arg1	numbers					688:694	the growing numbers	676:694	the growing numbers of homologous sequences in different databases	676:741	In this study, the growing numbers of homologous sequences in different databases were exploited in a comparative approach to investigate structural properties of EndoBI-1 and EndoBI-2 enzymes.
33488263	6	8	theme	enzymes	919:925	arg1	models					899:904	the complete and partial homology models	865:904	the complete and partial homology models of these two enzymes	865:925	Moreover, the complete and partial homology models of these two enzymes were generated and evaluated.
33488263	2	9	dep	longum	456:461	arg1	subsp					463:467	subsp	463:467	Bifidobacterium longum subsp	440:467	These N -glycans are indigestible by human infants and adults due to the lack of responsible glycosyl hydrolases and they act as selective prebiotics for a probiotic microorganism, Bifidobacterium longum subsp .
33488263	7	10	theme	docking	987:993	arg1	studies					995:1001	docking studies	987:1001	docking studies of the plus subsite ligand of these enzymes for further understanding on the substrate selectivity of EndoBI enzymes	987:1118	Selected models were used for docking studies of the plus subsite ligand of these enzymes for further understanding on the substrate selectivity of EndoBI enzymes.
33488263	2	11	theme	human	296:300	arg1	infants					302:308	human infants	296:308	human infants	296:308	These N -glycans are indigestible by human infants and adults due to the lack of responsible glycosyl hydrolases and they act as selective prebiotics for a probiotic microorganism, Bifidobacterium longum subsp .
33488263	1	12	theme	diacetylchitobiosyl	185:203	arg1	moieties					205:212	N-N'- diacetylchitobiosyl moieties	179:212	N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans	179:256	EndoBI-1 and EndoBI-2 are two endo- β-N- acetylglucosaminidase isoenzymes that cleave N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans.
33488263	5	13	theme	comparative	763:773	arg1	approach					775:782	a comparative approach	761:782	a comparative approach to investigate structural properties of EndoBI-1 and EndoBI-2 enzymes	761:852	In this study, the growing numbers of homologous sequences in different databases were exploited in a comparative approach to investigate structural properties of EndoBI-1 and EndoBI-2 enzymes.
33488263	6	14	theme	partial	882:888	arg1	models					899:904	the complete and partial homology models	865:904	the complete and partial homology models of these two enzymes	865:925	Moreover, the complete and partial homology models of these two enzymes were generated and evaluated.
33488263	4	15	theme	many	616:619	arg1	applications					647:658	many scientific and industrial applications	616:658	many scientific and industrial applications	616:658	The selectivity and the thermostability of EndoBI-1 and EndoBI-2 suggest that these enzymes may be useful for many scientific and industrial applications.
33488263	5	16	theme	EndoBI-2	837:844	arg1	properties					810:819	structural properties	799:819	structural properties of EndoBI-1 and EndoBI-2 enzymes	799:852	In this study, the growing numbers of homologous sequences in different databases were exploited in a comparative approach to investigate structural properties of EndoBI-1 and EndoBI-2 enzymes.
33488263	7	17	theme	enzymes	1112:1118	arg1	selectivity					1090:1100	the substrate selectivity	1076:1100	the substrate selectivity of EndoBI enzymes	1076:1118	Selected models were used for docking studies of the plus subsite ligand of these enzymes for further understanding on the substrate selectivity of EndoBI enzymes.
33488263	2	18	theme	selective	388:396	arg1	prebiotics					398:407	selective prebiotics	388:407	selective prebiotics for a probiotic microorganism	388:437	These N -glycans are indigestible by human infants and adults due to the lack of responsible glycosyl hydrolases and they act as selective prebiotics for a probiotic microorganism, Bifidobacterium longum subsp .
33488263	2	18	theme	selective	388:396	arg1	they					376:379	they	376:379	they	376:379	These N -glycans are indigestible by human infants and adults due to the lack of responsible glycosyl hydrolases and they act as selective prebiotics for a probiotic microorganism, Bifidobacterium longum subsp .
33488263	2	18	theme	selective	388:396	arg1	longum					456:461	Bifidobacterium longum	440:461	Bifidobacterium longum subsp	440:467	These N -glycans are indigestible by human infants and adults due to the lack of responsible glycosyl hydrolases and they act as selective prebiotics for a probiotic microorganism, Bifidobacterium longum subsp .
33488263	0	19	theme	Structural	0:9	arg1	insights					11:18	Structural insights	0:18	Structural insights of two novel N-acetyl-glucosaminidase enzymes	0:64	Structural insights of two novel N-acetyl-glucosaminidase enzymes through in silico methods.
33488263	7	20	theme	Selected	957:964	arg1	models					966:971	Selected models	957:971	Selected models	957:971	Selected models were used for docking studies of the plus subsite ligand of these enzymes for further understanding on the substrate selectivity of EndoBI enzymes.
33488263	5	21	dep	EndoBI-1	824:831	arg1	enzymes					846:852	enzymes	846:852	enzymes	846:852	In this study, the growing numbers of homologous sequences in different databases were exploited in a comparative approach to investigate structural properties of EndoBI-1 and EndoBI-2 enzymes.
33488263	2	22	theme	due	321:323	arg1	infants					302:308	human infants	296:308	human infants	296:308	These N -glycans are indigestible by human infants and adults due to the lack of responsible glycosyl hydrolases and they act as selective prebiotics for a probiotic microorganism, Bifidobacterium longum subsp .
33488263	1	23	theme	various	223:229	arg1	types					231:235	various types	223:235	various types of native N -glycans	223:256	EndoBI-1 and EndoBI-2 are two endo- β-N- acetylglucosaminidase isoenzymes that cleave N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans.
33488263	5	24	theme	different	723:731	arg1	databases					733:741	different databases	723:741	different databases	723:741	In this study, the growing numbers of homologous sequences in different databases were exploited in a comparative approach to investigate structural properties of EndoBI-1 and EndoBI-2 enzymes.
33488263	0	25	dep	in	74:75	arg1	silico					77:82	silico	77:82	silico	77:82	Structural insights of two novel N-acetyl-glucosaminidase enzymes through in silico methods.
33488263	7	26	used	used	978:981	arg2	models					966:971	Selected models	957:971	Selected models	957:971	Selected models were used for docking studies of the plus subsite ligand of these enzymes for further understanding on the substrate selectivity of EndoBI enzymes.
33488263	0	27	theme	N-acetyl-glucosaminidase	33:56	arg1	enzymes					58:64	two novel N-acetyl-glucosaminidase enzymes	23:64	two novel N-acetyl-glucosaminidase enzymes	23:64	Structural insights of two novel N-acetyl-glucosaminidase enzymes through in silico methods.
33488263	2	28	theme	responsible	340:350	arg1	hydrolases					361:370	responsible glycosyl hydrolases	340:370	responsible glycosyl hydrolases	340:370	These N -glycans are indigestible by human infants and adults due to the lack of responsible glycosyl hydrolases and they act as selective prebiotics for a probiotic microorganism, Bifidobacterium longum subsp .
33488263	7	29	theme	substrate	1080:1088	arg1	selectivity					1090:1100	the substrate selectivity	1076:1100	the substrate selectivity of EndoBI enzymes	1076:1118	Selected models were used for docking studies of the plus subsite ligand of these enzymes for further understanding on the substrate selectivity of EndoBI enzymes.
33488263	2	30	theme	probiotic	415:423	arg1	microorganism					425:437	a probiotic microorganism	413:437	a probiotic microorganism	413:437	These N -glycans are indigestible by human infants and adults due to the lack of responsible glycosyl hydrolases and they act as selective prebiotics for a probiotic microorganism, Bifidobacterium longum subsp .
33488263	0	31	theme	novel	27:31	arg1	enzymes					58:64	two novel N-acetyl-glucosaminidase enzymes	23:64	two novel N-acetyl-glucosaminidase enzymes	23:64	Structural insights of two novel N-acetyl-glucosaminidase enzymes through in silico methods.
33488263	4	32	theme	EndoBI-1	549:556	arg1	selectivity					510:520	The selectivity	506:520	The selectivity	506:520	The selectivity and the thermostability of EndoBI-1 and EndoBI-2 suggest that these enzymes may be useful for many scientific and industrial applications.
33488263	4	32	theme	EndoBI-1	549:556	arg1	thermostability					530:544	the thermostability	526:544	the thermostability of EndoBI-1 and EndoBI-2	526:569	The selectivity and the thermostability of EndoBI-1 and EndoBI-2 suggest that these enzymes may be useful for many scientific and industrial applications.
33488263	6	33	theme	homology	890:897	arg1	models					899:904	the complete and partial homology models	865:904	the complete and partial homology models of these two enzymes	865:925	Moreover, the complete and partial homology models of these two enzymes were generated and evaluated.
33488263	5	34	from	numbers	688:694	arg1	databases					733:741	different databases	723:741	different databases	723:741	In this study, the growing numbers of homologous sequences in different databases were exploited in a comparative approach to investigate structural properties of EndoBI-1 and EndoBI-2 enzymes.
33488263	7	35	theme	enzymes	1039:1045	arg1	ligand					1023:1028	the plus subsite ligand	1006:1028	the plus subsite ligand of these enzymes for further understanding on the substrate selectivity of EndoBI enzymes	1006:1118	Selected models were used for docking studies of the plus subsite ligand of these enzymes for further understanding on the substrate selectivity of EndoBI enzymes.
33488263	1	36	theme	native	240:245	arg1	-glycans					249:256	native N -glycans	240:256	native N -glycans	240:256	EndoBI-1 and EndoBI-2 are two endo- β-N- acetylglucosaminidase isoenzymes that cleave N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans.
33488263	5	37	theme	EndoBI-1	824:831	arg1	properties					810:819	structural properties	799:819	structural properties of EndoBI-1 and EndoBI-2 enzymes	799:852	In this study, the growing numbers of homologous sequences in different databases were exploited in a comparative approach to investigate structural properties of EndoBI-1 and EndoBI-2 enzymes.
33488263	1	38	located	found	214:218	arg1	types					231:235	various types	223:235	various types of native N -glycans	223:256	EndoBI-1 and EndoBI-2 are two endo- β-N- acetylglucosaminidase isoenzymes that cleave N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans.
33488263	1	38	located	found	214:218	arg2	moieties					205:212	N-N'- diacetylchitobiosyl moieties	179:212	N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans	179:256	EndoBI-1 and EndoBI-2 are two endo- β-N- acetylglucosaminidase isoenzymes that cleave N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans.
33488263	7	39	theme	further	1051:1057	arg1	understanding					1059:1071	further understanding	1051:1071	further understanding on the substrate selectivity of EndoBI enzymes	1051:1118	Selected models were used for docking studies of the plus subsite ligand of these enzymes for further understanding on the substrate selectivity of EndoBI enzymes.
33488263	2	40	theme	glycosyl	352:359	arg1	hydrolases					361:370	responsible glycosyl hydrolases	340:370	responsible glycosyl hydrolases	340:370	These N -glycans are indigestible by human infants and adults due to the lack of responsible glycosyl hydrolases and they act as selective prebiotics for a probiotic microorganism, Bifidobacterium longum subsp .
33488263	0	41	theme	enzymes	58:64	arg1	insights					11:18	Structural insights	0:18	Structural insights of two novel N-acetyl-glucosaminidase enzymes	0:64	Structural insights of two novel N-acetyl-glucosaminidase enzymes through in silico methods.
33488263	4	42	theme	EndoBI-2	562:569	arg1	selectivity					510:520	The selectivity	506:520	The selectivity	506:520	The selectivity and the thermostability of EndoBI-1 and EndoBI-2 suggest that these enzymes may be useful for many scientific and industrial applications.
33488263	4	42	theme	EndoBI-2	562:569	arg1	thermostability					530:544	the thermostability	526:544	the thermostability of EndoBI-1 and EndoBI-2	526:569	The selectivity and the thermostability of EndoBI-1 and EndoBI-2 suggest that these enzymes may be useful for many scientific and industrial applications.
33488263	3	43	theme	large	489:493	arg1	intestine					495:503	the large intestine	485:503	the large intestine	485:503	infantis , in the large intestine.
33488263	1	44	theme	endo-	123:127	arg1	EndoBI-2					106:113	EndoBI-2	106:113	EndoBI-2	106:113	EndoBI-1 and EndoBI-2 are two endo- β-N- acetylglucosaminidase isoenzymes that cleave N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans.
33488263	1	44	theme	endo-	123:127	arg1	EndoBI-1					93:100	EndoBI-1	93:100	EndoBI-1	93:100	EndoBI-1 and EndoBI-2 are two endo- β-N- acetylglucosaminidase isoenzymes that cleave N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans.
33488263	1	44	theme	endo-	123:127	arg1	isoenzymes					156:165	two endo- β-N- acetylglucosaminidase isoenzymes	119:165	two endo- β-N- acetylglucosaminidase isoenzymes that cleave N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans	119:256	EndoBI-1 and EndoBI-2 are two endo- β-N- acetylglucosaminidase isoenzymes that cleave N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans.
33488263	5	45	theme	homologous	699:708	arg1	sequences					710:718	homologous sequences	699:718	homologous sequences in different databases	699:741	In this study, the growing numbers of homologous sequences in different databases were exploited in a comparative approach to investigate structural properties of EndoBI-1 and EndoBI-2 enzymes.
33488263	6	46	theme	complete	869:876	arg1	models					899:904	the complete and partial homology models	865:904	the complete and partial homology models of these two enzymes	865:925	Moreover, the complete and partial homology models of these two enzymes were generated and evaluated.
33488263	3	47	dep	infantis	471:478	arg1	intestine					495:503	the large intestine	485:503	the large intestine	485:503	infantis , in the large intestine.
33488263	1	48	theme	β-N-	129:132	arg1	EndoBI-2					106:113	EndoBI-2	106:113	EndoBI-2	106:113	EndoBI-1 and EndoBI-2 are two endo- β-N- acetylglucosaminidase isoenzymes that cleave N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans.
33488263	1	48	theme	β-N-	129:132	arg1	EndoBI-1					93:100	EndoBI-1	93:100	EndoBI-1	93:100	EndoBI-1 and EndoBI-2 are two endo- β-N- acetylglucosaminidase isoenzymes that cleave N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans.
33488263	1	48	theme	β-N-	129:132	arg1	isoenzymes					156:165	two endo- β-N- acetylglucosaminidase isoenzymes	119:165	two endo- β-N- acetylglucosaminidase isoenzymes that cleave N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans	119:256	EndoBI-1 and EndoBI-2 are two endo- β-N- acetylglucosaminidase isoenzymes that cleave N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans.
33488263	1	49	theme	N	247:247	arg1	-glycans					249:256	native N -glycans	240:256	native N -glycans	240:256	EndoBI-1 and EndoBI-2 are two endo- β-N- acetylglucosaminidase isoenzymes that cleave N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans.
33488263	5	50	theme	sequences	710:718	arg1	numbers					688:694	the growing numbers	676:694	the growing numbers of homologous sequences in different databases	676:741	In this study, the growing numbers of homologous sequences in different databases were exploited in a comparative approach to investigate structural properties of EndoBI-1 and EndoBI-2 enzymes.
33488263	7	51	theme	subsite	1015:1021	arg1	ligand					1023:1028	the plus subsite ligand	1006:1028	the plus subsite ligand of these enzymes for further understanding on the substrate selectivity of EndoBI enzymes	1006:1118	Selected models were used for docking studies of the plus subsite ligand of these enzymes for further understanding on the substrate selectivity of EndoBI enzymes.
33488263	1	52	theme	acetylglucosaminidase	134:154	arg1	EndoBI-2					106:113	EndoBI-2	106:113	EndoBI-2	106:113	EndoBI-1 and EndoBI-2 are two endo- β-N- acetylglucosaminidase isoenzymes that cleave N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans.
33488263	1	52	theme	acetylglucosaminidase	134:154	arg1	EndoBI-1					93:100	EndoBI-1	93:100	EndoBI-1	93:100	EndoBI-1 and EndoBI-2 are two endo- β-N- acetylglucosaminidase isoenzymes that cleave N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans.
33488263	1	52	theme	acetylglucosaminidase	134:154	arg1	isoenzymes					156:165	two endo- β-N- acetylglucosaminidase isoenzymes	119:165	two endo- β-N- acetylglucosaminidase isoenzymes that cleave N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans	119:256	EndoBI-1 and EndoBI-2 are two endo- β-N- acetylglucosaminidase isoenzymes that cleave N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans.
33488263	1	53	theme	-glycans	249:256	arg1	types					231:235	various types	223:235	various types of native N -glycans	223:256	EndoBI-1 and EndoBI-2 are two endo- β-N- acetylglucosaminidase isoenzymes that cleave N-N'- diacetylchitobiosyl moieties found in various types of native N -glycans.
33488263	0	54	dep	insights	11:18	arg1	methods					84:90	in silico methods	74:90	in silico methods	74:90	Structural insights of two novel N-acetyl-glucosaminidase enzymes through in silico methods.
33488263	7	55	theme	ligand	1023:1028	arg1	studies					995:1001	docking studies	987:1001	docking studies of the plus subsite ligand of these enzymes for further understanding on the substrate selectivity of EndoBI enzymes	987:1118	Selected models were used for docking studies of the plus subsite ligand of these enzymes for further understanding on the substrate selectivity of EndoBI enzymes.
33488263	4	56	theme	industrial	636:645	arg1	applications					647:658	many scientific and industrial applications	616:658	many scientific and industrial applications	616:658	The selectivity and the thermostability of EndoBI-1 and EndoBI-2 suggest that these enzymes may be useful for many scientific and industrial applications.
33488263	0	57	theme	in	74:75	arg1	methods					84:90	in silico methods	74:90	in silico methods	74:90	Structural insights of two novel N-acetyl-glucosaminidase enzymes through in silico methods.
33488263	7	58	from	understanding	1059:1071	arg1	selectivity					1090:1100	the substrate selectivity	1076:1100	the substrate selectivity of EndoBI enzymes	1076:1118	Selected models were used for docking studies of the plus subsite ligand of these enzymes for further understanding on the substrate selectivity of EndoBI enzymes.
33488263	2	59	theme	N	265:265	arg1	-glycans					267:274	These N -glycans	259:274	These N -glycans	259:274	These N -glycans are indigestible by human infants and adults due to the lack of responsible glycosyl hydrolases and they act as selective prebiotics for a probiotic microorganism, Bifidobacterium longum subsp .
33488263	4	60	theme	scientific	621:630	arg1	applications					647:658	many scientific and industrial applications	616:658	many scientific and industrial applications	616:658	The selectivity and the thermostability of EndoBI-1 and EndoBI-2 suggest that these enzymes may be useful for many scientific and industrial applications.
32536403	1	0	dep	imine	279:283	arg1	the					275:277	the	275:277	the	275:277	A new self-crosslinked composite hydrogel is prepared with chitosan (CS) and cationic guar gum (CGG), based on the imine and acetal chemistry for gelation.
32536403	1	1	theme	self-crosslinked	170:185	arg1	hydrogel					197:204	A new self-crosslinked composite hydrogel	164:204	A new self-crosslinked composite hydrogel	164:204	A new self-crosslinked composite hydrogel is prepared with chitosan (CS) and cationic guar gum (CGG), based on the imine and acetal chemistry for gelation.
32536403	6	2	theme	specific	1031:1038	arg1	performance					1013:1023	excellent capacitive performance	992:1023	excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g)	992:1070	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	6	2	theme	specific	1031:1038	arg1	F/g					1067:1069	best specific capacitance of 302.2 ± 4.9 F/g	1026:1069	best specific capacitance of 302.2 ± 4.9 F/g	1026:1069	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	5	3	theme	phosphate-adsorbed	795:812	arg1	hydrogels					814:822	the phosphate-adsorbed hydrogels	791:822	the phosphate-adsorbed hydrogels	791:822	Considering their porous structure and nitrogen/phosphorus heteroatoms doping, the phosphate-adsorbed hydrogels are made into porous N,P doped carbon aerogels that can be potentially used as electrodes for a supercapacitor.
32536403	1	4	theme	composite	187:195	arg1	hydrogel					197:204	A new self-crosslinked composite hydrogel	164:204	A new self-crosslinked composite hydrogel	164:204	A new self-crosslinked composite hydrogel is prepared with chitosan (CS) and cationic guar gum (CGG), based on the imine and acetal chemistry for gelation.
32536403	6	5	theme	best	1026:1029	arg1	performance					1013:1023	excellent capacitive performance	992:1023	excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g)	992:1070	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	6	5	theme	best	1026:1029	arg1	F/g					1067:1069	best specific capacitance of 302.2 ± 4.9 F/g	1026:1069	best specific capacitance of 302.2 ± 4.9 F/g	1026:1069	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	0	6	theme	phosphate-containing	107:126	arg1	treatment					134:142	phosphate-containing water treatment	107:142	phosphate-containing water treatment	107:142	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile uses in phosphate-containing water treatment and energy storage.
32536403	0	7	link	self-crosslinked	18:33	arg1	gum					58:60	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile	0:97	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile	0:97	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile uses in phosphate-containing water treatment and energy storage.
32536403	5	8	used	used	895:898	arg2	electrodes					903:912	electrodes	903:912	electrodes for a supercapacitor	903:933	Considering their porous structure and nitrogen/phosphorus heteroatoms doping, the phosphate-adsorbed hydrogels are made into porous N,P doped carbon aerogels that can be potentially used as electrodes for a supercapacitor.
32536403	5	8	used	used	895:898	arg2	aerogels					862:869	porous N,P doped carbon aerogels	838:869	porous N,P doped carbon aerogels that can be potentially used as electrodes for a supercapacitor	838:933	Considering their porous structure and nitrogen/phosphorus heteroatoms doping, the phosphate-adsorbed hydrogels are made into porous N,P doped carbon aerogels that can be potentially used as electrodes for a supercapacitor.
32536403	3	9	theme	adsorption	510:519	arg1	process					521:527	an adsorption process	507:527	an adsorption process	507:527	The hydrogel is effective in removing phosphate from wastewater through an adsorption process, during which KH2PO4 is used as a phosphate model.
32536403	5	10	theme	nitrogen/phosphorus	751:769	arg1	heteroatoms					771:781	nitrogen/phosphorus heteroatoms doping	751:788	nitrogen/phosphorus heteroatoms doping	751:788	Considering their porous structure and nitrogen/phosphorus heteroatoms doping, the phosphate-adsorbed hydrogels are made into porous N,P doped carbon aerogels that can be potentially used as electrodes for a supercapacitor.
32536403	5	11	theme	carbon	855:860	arg1	aerogels					862:869	porous N,P doped carbon aerogels	838:869	porous N,P doped carbon aerogels that can be potentially used as electrodes for a supercapacitor	838:933	Considering their porous structure and nitrogen/phosphorus heteroatoms doping, the phosphate-adsorbed hydrogels are made into porous N,P doped carbon aerogels that can be potentially used as electrodes for a supercapacitor.
32536403	5	11	theme	carbon	855:860	arg1	electrodes					903:912	electrodes	903:912	electrodes for a supercapacitor	903:933	Considering their porous structure and nitrogen/phosphorus heteroatoms doping, the phosphate-adsorbed hydrogels are made into porous N,P doped carbon aerogels that can be potentially used as electrodes for a supercapacitor.
32536403	6	12	theme	good	1084:1087	arg1	stability					1097:1105	good cycling stability	1084:1105	good cycling stability	1084:1105	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	0	13	theme	water	128:132	arg1	treatment					134:142	phosphate-containing water treatment	107:142	phosphate-containing water treatment	107:142	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile uses in phosphate-containing water treatment and energy storage.
32536403	5	14	theme	porous	838:843	arg1	N					845:845	porous N	838:845	porous N	838:845	Considering their porous structure and nitrogen/phosphorus heteroatoms doping, the phosphate-adsorbed hydrogels are made into porous N,P doped carbon aerogels that can be potentially used as electrodes for a supercapacitor.
32536403	5	14	theme	porous	838:843	arg1	P					847:847	P	847:847	P	847:847	Considering their porous structure and nitrogen/phosphorus heteroatoms doping, the phosphate-adsorbed hydrogels are made into porous N,P doped carbon aerogels that can be potentially used as electrodes for a supercapacitor.
32536403	6	15	theme	cycling	1089:1095	arg1	stability					1097:1105	good cycling stability	1084:1105	good cycling stability	1084:1105	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	0	16	theme	multifunctional	2:16	arg1	gum					58:60	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile	0:97	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile	0:97	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile uses in phosphate-containing water treatment and energy storage.
32536403	3	17	theme	phosphate	563:571	arg1	KH2PO4					543:548	KH2PO4	543:548	KH2PO4	543:548	The hydrogel is effective in removing phosphate from wastewater through an adsorption process, during which KH2PO4 is used as a phosphate model.
32536403	3	17	theme	phosphate	563:571	arg1	model					573:577	a phosphate model	561:577	a phosphate model	561:577	The hydrogel is effective in removing phosphate from wastewater through an adsorption process, during which KH2PO4 is used as a phosphate model.
32536403	3	18	used	used	553:556	arg2	model					573:577	a phosphate model	561:577	a phosphate model	561:577	The hydrogel is effective in removing phosphate from wastewater through an adsorption process, during which KH2PO4 is used as a phosphate model.
32536403	3	18	used	used	553:556	arg2	KH2PO4					543:548	KH2PO4	543:548	KH2PO4	543:548	The hydrogel is effective in removing phosphate from wastewater through an adsorption process, during which KH2PO4 is used as a phosphate model.
32536403	5	19	theme	N	845:845	arg1	aerogels					862:869	porous N,P doped carbon aerogels	838:869	porous N,P doped carbon aerogels that can be potentially used as electrodes for a supercapacitor	838:933	Considering their porous structure and nitrogen/phosphorus heteroatoms doping, the phosphate-adsorbed hydrogels are made into porous N,P doped carbon aerogels that can be potentially used as electrodes for a supercapacitor.
32536403	5	19	theme	N	845:845	arg1	electrodes					903:912	electrodes	903:912	electrodes for a supercapacitor	903:933	Considering their porous structure and nitrogen/phosphorus heteroatoms doping, the phosphate-adsorbed hydrogels are made into porous N,P doped carbon aerogels that can be potentially used as electrodes for a supercapacitor.
32536403	6	20	theme	capacitive	1002:1011	arg1	performance					1013:1023	excellent capacitive performance	992:1023	excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g)	992:1070	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	6	20	theme	capacitive	1002:1011	arg1	F/g					1067:1069	best specific capacitance of 302.2 ± 4.9 F/g	1026:1069	best specific capacitance of 302.2 ± 4.9 F/g	1026:1069	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	4	21	theme	multilayered	655:666	arg1	it					647:648	it	647:648	it	647:648	The adsorption complies with the Freundlich model, indicating that it is a multilayered process with complex adsorption mechanisms.
32536403	4	21	theme	multilayered	655:666	arg1	process					668:674	a multilayered process	653:674	a multilayered process with complex adsorption mechanisms	653:709	The adsorption complies with the Freundlich model, indicating that it is a multilayered process with complex adsorption mechanisms.
32536403	0	22	theme	chitosan/cationic	35:51	arg1	gum					58:60	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile	0:97	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile	0:97	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile uses in phosphate-containing water treatment and energy storage.
32536403	6	23	theme	excellent	992:1000	arg1	performance					1013:1023	excellent capacitive performance	992:1023	excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g)	992:1070	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	6	23	theme	excellent	992:1000	arg1	F/g					1067:1069	best specific capacitance of 302.2 ± 4.9 F/g	1026:1069	best specific capacitance of 302.2 ± 4.9 F/g	1026:1069	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	0	24	theme	energy	148:153	arg1	storage					155:161	energy storage	148:161	energy storage	148:161	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile uses in phosphate-containing water treatment and energy storage.
32536403	5	25	theme	doped	849:853	arg1	aerogels					862:869	porous N,P doped carbon aerogels	838:869	porous N,P doped carbon aerogels that can be potentially used as electrodes for a supercapacitor	838:933	Considering their porous structure and nitrogen/phosphorus heteroatoms doping, the phosphate-adsorbed hydrogels are made into porous N,P doped carbon aerogels that can be potentially used as electrodes for a supercapacitor.
32536403	5	25	theme	doped	849:853	arg1	electrodes					903:912	electrodes	903:912	electrodes for a supercapacitor	903:933	Considering their porous structure and nitrogen/phosphorus heteroatoms doping, the phosphate-adsorbed hydrogels are made into porous N,P doped carbon aerogels that can be potentially used as electrodes for a supercapacitor.
32536403	0	26	theme	self-crosslinked	18:33	arg1	gum					58:60	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile	0:97	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile	0:97	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile uses in phosphate-containing water treatment and energy storage.
32536403	6	27	theme	of	1052:1053	arg1	performance					1013:1023	excellent capacitive performance	992:1023	excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g)	992:1070	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	6	27	theme	of	1052:1053	arg1	F/g					1067:1069	best specific capacitance of 302.2 ± 4.9 F/g	1026:1069	best specific capacitance of 302.2 ± 4.9 F/g	1026:1069	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	4	28	with	process	668:674	arg1	mechanisms					700:709	complex adsorption mechanisms	681:709	complex adsorption mechanisms	681:709	The adsorption complies with the Freundlich model, indicating that it is a multilayered process with complex adsorption mechanisms.
32536403	4	29	theme	adsorption	689:698	arg1	mechanisms					700:709	complex adsorption mechanisms	681:709	complex adsorption mechanisms	681:709	The adsorption complies with the Freundlich model, indicating that it is a multilayered process with complex adsorption mechanisms.
32536403	2	30	theme	compressive	413:423	arg1	strength					425:432	good compressive strength	408:432	good compressive strength	408:432	The CS/CGG hydrogel exhibits thermal/pH responsiveness, injectability, adhesiveness and good compressive strength.
32536403	2	31	theme	good	408:411	arg1	strength					425:432	good compressive strength	408:432	good compressive strength	408:432	The CS/CGG hydrogel exhibits thermal/pH responsiveness, injectability, adhesiveness and good compressive strength.
32536403	0	32	theme	guar	53:56	arg1	gum					58:60	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile	0:97	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile	0:97	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile uses in phosphate-containing water treatment and energy storage.
32536403	6	33	theme	carbon	968:973	arg1	aerogels					975:982	these carbon aerogels	962:982	these carbon aerogels	962:982	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	5	34	theme	porous	730:735	arg1	structure					737:745	their porous structure	724:745	their porous structure	724:745	Considering their porous structure and nitrogen/phosphorus heteroatoms doping, the phosphate-adsorbed hydrogels are made into porous N,P doped carbon aerogels that can be potentially used as electrodes for a supercapacitor.
32536403	6	35	contain	possess	984:990	arg2	performance					1013:1023	excellent capacitive performance	992:1023	excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g)	992:1070	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	6	35	contain	possess	984:990	arg2	F/g					1067:1069	best specific capacitance of 302.2 ± 4.9 F/g	1026:1069	best specific capacitance of 302.2 ± 4.9 F/g	1026:1069	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	6	35	contain	possess	984:990	arg2	stability					1097:1105	good cycling stability	1084:1105	good cycling stability	1084:1105	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	6	35	contain	possess	984:990	arg1	aerogels					975:982	these carbon aerogels	962:982	these carbon aerogels	962:982	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	1	36	theme	acetal	289:294	arg1	chemistry					296:304	acetal chemistry	289:304	acetal chemistry	289:304	A new self-crosslinked composite hydrogel is prepared with chitosan (CS) and cationic guar gum (CGG), based on the imine and acetal chemistry for gelation.
32536403	0	37	dep	gum	58:60	arg1	versatile					89:97	versatile	89:97	versatile	89:97	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile uses in phosphate-containing water treatment and energy storage.
32536403	0	37	dep	gum	58:60	arg1	hydrogel					72:79	composite hydrogel	62:79	composite hydrogel	62:79	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile uses in phosphate-containing water treatment and energy storage.
32536403	2	38	theme	CS/CGG	324:329	arg1	hydrogel					331:338	The CS/CGG hydrogel	320:338	The CS/CGG hydrogel	320:338	The CS/CGG hydrogel exhibits thermal/pH responsiveness, injectability, adhesiveness and good compressive strength.
32536403	0	39	theme	composite	62:70	arg1	hydrogel					72:79	composite hydrogel	62:79	composite hydrogel	62:79	A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile uses in phosphate-containing water treatment and energy storage.
32536403	1	40	link	self-crosslinked	170:185	arg1	hydrogel					197:204	A new self-crosslinked composite hydrogel	164:204	A new self-crosslinked composite hydrogel	164:204	A new self-crosslinked composite hydrogel is prepared with chitosan (CS) and cationic guar gum (CGG), based on the imine and acetal chemistry for gelation.
32536403	6	41	theme	charging/discharging	1127:1146	arg1	times					1118:1122	5000 times	1113:1122	5000 times of charging/discharging	1113:1146	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	6	42	theme	±	1061:1061	arg1	performance					1013:1023	excellent capacitive performance	992:1023	excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g)	992:1070	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	6	42	theme	±	1061:1061	arg1	F/g					1067:1069	best specific capacitance of 302.2 ± 4.9 F/g	1026:1069	best specific capacitance of 302.2 ± 4.9 F/g	1026:1069	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	2	43	theme	thermal/pH	349:358	arg1	responsiveness					360:373	thermal/pH responsiveness	349:373	thermal/pH responsiveness	349:373	The CS/CGG hydrogel exhibits thermal/pH responsiveness, injectability, adhesiveness and good compressive strength.
32536403	1	44	theme	cationic	241:248	arg1	CGG					260:262	CGG	260:262	CGG	260:262	A new self-crosslinked composite hydrogel is prepared with chitosan (CS) and cationic guar gum (CGG), based on the imine and acetal chemistry for gelation.
32536403	1	44	theme	cationic	241:248	arg1	gum					255:257	cationic guar gum	241:257	cationic guar gum (CGG)	241:263	A new self-crosslinked composite hydrogel is prepared with chitosan (CS) and cationic guar gum (CGG), based on the imine and acetal chemistry for gelation.
32536403	5	45	theme	doping	783:788	arg1	heteroatoms					771:781	nitrogen/phosphorus heteroatoms doping	751:788	nitrogen/phosphorus heteroatoms doping	751:788	Considering their porous structure and nitrogen/phosphorus heteroatoms doping, the phosphate-adsorbed hydrogels are made into porous N,P doped carbon aerogels that can be potentially used as electrodes for a supercapacitor.
32536403	4	46	theme	complex	681:687	arg1	mechanisms					700:709	complex adsorption mechanisms	681:709	complex adsorption mechanisms	681:709	The adsorption complies with the Freundlich model, indicating that it is a multilayered process with complex adsorption mechanisms.
32536403	1	47	theme	guar	250:253	arg1	CGG					260:262	CGG	260:262	CGG	260:262	A new self-crosslinked composite hydrogel is prepared with chitosan (CS) and cationic guar gum (CGG), based on the imine and acetal chemistry for gelation.
32536403	1	47	theme	guar	250:253	arg1	gum					255:257	cationic guar gum	241:257	cationic guar gum (CGG)	241:263	A new self-crosslinked composite hydrogel is prepared with chitosan (CS) and cationic guar gum (CGG), based on the imine and acetal chemistry for gelation.
32536403	6	48	theme	capacitance	1040:1050	arg1	performance					1013:1023	excellent capacitive performance	992:1023	excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g)	992:1070	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	6	48	theme	capacitance	1040:1050	arg1	F/g					1067:1069	best specific capacitance of 302.2 ± 4.9 F/g	1026:1069	best specific capacitance of 302.2 ± 4.9 F/g	1026:1069	The results indicate that these carbon aerogels possess excellent capacitive performance (best specific capacitance of 302.2 ± 4.9 F/g), as well as good cycling stability after 5000 times of charging/discharging.
32536403	1	49	theme	new	166:168	arg1	hydrogel					197:204	A new self-crosslinked composite hydrogel	164:204	A new self-crosslinked composite hydrogel	164:204	A new self-crosslinked composite hydrogel is prepared with chitosan (CS) and cationic guar gum (CGG), based on the imine and acetal chemistry for gelation.
32536403	4	50	theme	Freundlich	613:622	arg1	model					624:628	the Freundlich model	609:628	the Freundlich model	609:628	The adsorption complies with the Freundlich model, indicating that it is a multilayered process with complex adsorption mechanisms.
32540551	6	0	theme	LPS	891:893	arg1	constructs					895:904	both native and modified LPS constructs	866:904	both native and modified LPS constructs	866:904	Electrolytes and low pHs induced OMV aggregation for both native and modified LPS constructs, approaching a calculated fusion efficiency of ~25% (i.e. ~1/4 of collision events lead to fusion).
32540551	5	1	attach	isolated	632:639	arg2	OMVs					627:630	OMVs	627:630	OMVs isolated from native E. coli (Nissle 1917) and lipopolysaccharide (LPS) modified E. coli (ClearColi) strains to evaluate the effect of lipid type on vesicle aggregation and fusion	627:810	Vesicle aggregation and fusion kinetics were investigated for OMVs isolated from native E. coli (Nissle 1917) and lipopolysaccharide (LPS) modified E. coli (ClearColi) strains to evaluate the effect of lipid type on vesicle aggregation and fusion.
32540551	5	1	attach	isolated	632:639	arg1	lipopolysaccharide					679:696	lipopolysaccharide	679:696	lipopolysaccharide (LPS)	679:702	Vesicle aggregation and fusion kinetics were investigated for OMVs isolated from native E. coli (Nissle 1917) and lipopolysaccharide (LPS) modified E. coli (ClearColi) strains to evaluate the effect of lipid type on vesicle aggregation and fusion.
32540551	5	1	attach	isolated	632:639	arg1	Nissle					662:667	Nissle	662:667	Nissle	662:667	Vesicle aggregation and fusion kinetics were investigated for OMVs isolated from native E. coli (Nissle 1917) and lipopolysaccharide (LPS) modified E. coli (ClearColi) strains to evaluate the effect of lipid type on vesicle aggregation and fusion.
32540551	5	1	attach	isolated	632:639	arg1	LPS					699:701	LPS	699:701	LPS	699:701	Vesicle aggregation and fusion kinetics were investigated for OMVs isolated from native E. coli (Nissle 1917) and lipopolysaccharide (LPS) modified E. coli (ClearColi) strains to evaluate the effect of lipid type on vesicle aggregation and fusion.
32540551	5	1	attach	isolated	632:639	arg1	coli					656:659	E. coli	653:659	native E. coli (Nissle 1917)	646:673	Vesicle aggregation and fusion kinetics were investigated for OMVs isolated from native E. coli (Nissle 1917) and lipopolysaccharide (LPS) modified E. coli (ClearColi) strains to evaluate the effect of lipid type on vesicle aggregation and fusion.
32540551	6	2	theme	collision	972:980	arg1	events					982:987	collision events	972:987	collision events	972:987	Electrolytes and low pHs induced OMV aggregation for both native and modified LPS constructs, approaching a calculated fusion efficiency of ~25% (i.e. ~1/4 of collision events lead to fusion).
32540551	0	3	theme	Experimental	69:80	arg1	analysis					98:105	Experimental and theoretical analysis	69:105	Induced fusion and aggregation of bacterial outer membrane vesicles: Experimental and theoretical analysis.	0:106	Induced fusion and aggregation of bacterial outer membrane vesicles: Experimental and theoretical analysis.
32540551	4	4	theme	Derjaguin-Landau-Verwey-Overbeek	498:529	arg1	model					558:562	the Derjaguin-Landau-Verwey-Overbeek (DLVO) colloidal stability model	494:562	the Derjaguin-Landau-Verwey-Overbeek (DLVO) colloidal stability model	494:562	Here we measure the pH and salt-induced aggregation and fusion of OMVs and analyze the processes against the Derjaguin-Landau-Verwey-Overbeek (DLVO) colloidal stability model.
32540551	6	5	dep	%	956:956	arg1	i.e. ~1/4					959:967	i.e. ~1/4	959:967	i.e. ~1/4	959:967	Electrolytes and low pHs induced OMV aggregation for both native and modified LPS constructs, approaching a calculated fusion efficiency of ~25% (i.e. ~1/4 of collision events lead to fusion).
32540551	5	6	theme	lipid	767:771	arg1	type					773:776	lipid type	767:776	lipid type	767:776	Vesicle aggregation and fusion kinetics were investigated for OMVs isolated from native E. coli (Nissle 1917) and lipopolysaccharide (LPS) modified E. coli (ClearColi) strains to evaluate the effect of lipid type on vesicle aggregation and fusion.
32540551	9	7	theme	conditions	1459:1468	arg1	conditions					1459:1468	conditions	1459:1468	conditions under which investigators can induce OMVs to fuse and make various combinations of vesicle compositions	1459:1572	The outcome of the work is a defined set of conditions under which investigators can induce OMVs to fuse and make various combinations of vesicle compositions.
32540551	9	7	theme	conditions	1459:1468	arg1	set					1452:1454	a defined set	1442:1454	a defined set of conditions under which investigators can induce OMVs to fuse and make various combinations of vesicle compositions	1442:1572	The outcome of the work is a defined set of conditions under which investigators can induce OMVs to fuse and make various combinations of vesicle compositions.
32540551	9	7	theme	conditions	1459:1468	arg1	outcome					1419:1425	The outcome	1415:1425	The outcome of the work	1415:1437	The outcome of the work is a defined set of conditions under which investigators can induce OMVs to fuse and make various combinations of vesicle compositions.
32540551	5	8	theme	coli	716:719	arg1	strains					733:739	modified E. coli (ClearColi) strains	704:739	modified E. coli (ClearColi) strains	704:739	Vesicle aggregation and fusion kinetics were investigated for OMVs isolated from native E. coli (Nissle 1917) and lipopolysaccharide (LPS) modified E. coli (ClearColi) strains to evaluate the effect of lipid type on vesicle aggregation and fusion.
32540551	8	9	theme	monovalent	1391:1400	arg1	counterions					1402:1412	monovalent counterions	1391:1412	monovalent counterions	1391:1412	The lipid composition of the OMVs from Nissle negatively impacted fusion in the presence of electrolytes, causing higher deviations from DLVO-predicted critical coagulation concentrations with monovalent counterions.
32540551	6	10	theme	native	871:876	arg1	constructs					895:904	both native and modified LPS constructs	866:904	both native and modified LPS constructs	866:904	Electrolytes and low pHs induced OMV aggregation for both native and modified LPS constructs, approaching a calculated fusion efficiency of ~25% (i.e. ~1/4 of collision events lead to fusion).
32540551	9	11	theme	compositions	1561:1572	arg1	combinations					1537:1548	various combinations	1529:1548	various combinations of vesicle compositions	1529:1572	The outcome of the work is a defined set of conditions under which investigators can induce OMVs to fuse and make various combinations of vesicle compositions.
32540551	0	12	theme	theoretical	86:96	arg1	analysis					98:105	Experimental and theoretical analysis	69:105	Induced fusion and aggregation of bacterial outer membrane vesicles: Experimental and theoretical analysis.	0:106	Induced fusion and aggregation of bacterial outer membrane vesicles: Experimental and theoretical analysis.
32540551	5	13	theme	vesicle	781:787	arg1	aggregation					789:799	vesicle aggregation	781:799	vesicle aggregation	781:799	Vesicle aggregation and fusion kinetics were investigated for OMVs isolated from native E. coli (Nissle 1917) and lipopolysaccharide (LPS) modified E. coli (ClearColi) strains to evaluate the effect of lipid type on vesicle aggregation and fusion.
32540551	8	14	theme	electrolytes	1290:1301	arg1	presence					1278:1285	the presence	1274:1285	the presence of electrolytes	1274:1301	The lipid composition of the OMVs from Nissle negatively impacted fusion in the presence of electrolytes, causing higher deviations from DLVO-predicted critical coagulation concentrations with monovalent counterions.
32540551	8	15	with	concentrations	1371:1384	arg1	counterions					1402:1412	monovalent counterions	1391:1412	monovalent counterions	1391:1412	The lipid composition of the OMVs from Nissle negatively impacted fusion in the presence of electrolytes, causing higher deviations from DLVO-predicted critical coagulation concentrations with monovalent counterions.
32540551	2	16	theme	induced	288:294	arg1	fusion					296:301	induced fusion	288:301	induced fusion of OMV populations	288:320	The diversity of exogenous antigens delivered by OMVs can be enhanced by induced fusion of OMV populations.
32540551	4	17	theme	OMVs	455:458	arg1	fusion					445:450	fusion	445:450	fusion	445:450	Here we measure the pH and salt-induced aggregation and fusion of OMVs and analyze the processes against the Derjaguin-Landau-Verwey-Overbeek (DLVO) colloidal stability model.
32540551	4	17	theme	OMVs	455:458	arg1	pH					409:410	pH	409:410	pH	409:410	Here we measure the pH and salt-induced aggregation and fusion of OMVs and analyze the processes against the Derjaguin-Landau-Verwey-Overbeek (DLVO) colloidal stability model.
32540551	4	17	theme	OMVs	455:458	arg1	aggregation					429:439	salt-induced aggregation	416:439	salt-induced aggregation	416:439	Here we measure the pH and salt-induced aggregation and fusion of OMVs and analyze the processes against the Derjaguin-Landau-Verwey-Overbeek (DLVO) colloidal stability model.
32540551	7	18	theme	Nissle	1055:1060	arg1	OMVs					1062:1065	Nissle OMVs	1055:1065	Nissle OMVs	1055:1065	However, high fusion efficiency was achieved for Nissle OMVs solely with decreased pH as opposed to a combination of low pH and increased divalent counterion concentration for ClearColi OMVs.
32540551	8	19	from	Nissle	1237:1242	arg1	composition					1208:1218	The lipid composition	1198:1218	The lipid composition of the OMVs from Nissle	1198:1242	The lipid composition of the OMVs from Nissle negatively impacted fusion in the presence of electrolytes, causing higher deviations from DLVO-predicted critical coagulation concentrations with monovalent counterions.
32540551	8	19	from	Nissle	1237:1242	arg1	OMVs					1227:1230	the OMVs	1223:1230	the OMVs from Nissle	1223:1242	The lipid composition of the OMVs from Nissle negatively impacted fusion in the presence of electrolytes, causing higher deviations from DLVO-predicted critical coagulation concentrations with monovalent counterions.
32540551	8	20	theme	higher	1312:1317	arg1	deviations					1319:1328	higher deviations	1312:1328	higher deviations from DLVO-predicted critical coagulation concentrations with monovalent counterions	1312:1412	The lipid composition of the OMVs from Nissle negatively impacted fusion in the presence of electrolytes, causing higher deviations from DLVO-predicted critical coagulation concentrations with monovalent counterions.
32540551	9	21	theme	work	1434:1437	arg1	conditions					1459:1468	conditions	1459:1468	conditions under which investigators can induce OMVs to fuse and make various combinations of vesicle compositions	1459:1572	The outcome of the work is a defined set of conditions under which investigators can induce OMVs to fuse and make various combinations of vesicle compositions.
32540551	9	21	theme	work	1434:1437	arg1	set					1452:1454	a defined set	1442:1454	a defined set of conditions under which investigators can induce OMVs to fuse and make various combinations of vesicle compositions	1442:1572	The outcome of the work is a defined set of conditions under which investigators can induce OMVs to fuse and make various combinations of vesicle compositions.
32540551	9	21	theme	work	1434:1437	arg1	outcome					1419:1425	The outcome	1415:1425	The outcome of the work	1415:1437	The outcome of the work is a defined set of conditions under which investigators can induce OMVs to fuse and make various combinations of vesicle compositions.
32540551	5	22	theme	type	773:776	arg1	effect					757:762	the effect	753:762	the effect of lipid type on vesicle aggregation and fusion	753:810	Vesicle aggregation and fusion kinetics were investigated for OMVs isolated from native E. coli (Nissle 1917) and lipopolysaccharide (LPS) modified E. coli (ClearColi) strains to evaluate the effect of lipid type on vesicle aggregation and fusion.
32540551	3	23	theme	OMVs	383:386	arg1	fusion					363:368	induced fusion	355:368	induced fusion of bacterial OMVs	355:386	To date there are no reports of induced fusion of bacterial OMVs.
32540551	8	24	from	concentrations	1371:1384	arg1	deviations					1319:1328	higher deviations	1312:1328	higher deviations from DLVO-predicted critical coagulation concentrations with monovalent counterions	1312:1412	The lipid composition of the OMVs from Nissle negatively impacted fusion in the presence of electrolytes, causing higher deviations from DLVO-predicted critical coagulation concentrations with monovalent counterions.
32540551	1	25	theme	promising	178:186	arg1	vesicles					158:165	Recombinantly engineered bacterial outer membrane vesicles	108:165	Recombinantly engineered bacterial outer membrane vesicles (OMVs)	108:172	Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles.
32540551	1	25	theme	promising	178:186	arg1	vehicles					205:212	promising vaccine delivery vehicles	178:212	promising vaccine delivery vehicles	178:212	Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles.
32540551	3	26	theme	bacterial	373:381	arg1	OMVs					383:386	bacterial OMVs	373:386	bacterial OMVs	373:386	To date there are no reports of induced fusion of bacterial OMVs.
32540551	8	27	theme	coagulation	1359:1369	arg1	concentrations					1371:1384	DLVO-predicted critical coagulation concentrations	1335:1384	DLVO-predicted critical coagulation concentrations with monovalent counterions	1335:1412	The lipid composition of the OMVs from Nissle negatively impacted fusion in the presence of electrolytes, causing higher deviations from DLVO-predicted critical coagulation concentrations with monovalent counterions.
32540551	9	28	theme	various	1529:1535	arg1	combinations					1537:1548	various combinations	1529:1548	various combinations of vesicle compositions	1529:1572	The outcome of the work is a defined set of conditions under which investigators can induce OMVs to fuse and make various combinations of vesicle compositions.
32540551	1	29	theme	vaccine	188:194	arg1	vesicles					158:165	Recombinantly engineered bacterial outer membrane vesicles	108:165	Recombinantly engineered bacterial outer membrane vesicles (OMVs)	108:172	Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles.
32540551	1	29	theme	vaccine	188:194	arg1	vehicles					205:212	promising vaccine delivery vehicles	178:212	promising vaccine delivery vehicles	178:212	Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles.
32540551	0	30	theme	Induced	0:6	arg1	fusion					8:13	fusion	8:13	fusion	8:13	Induced fusion and aggregation of bacterial outer membrane vesicles: Experimental and theoretical analysis.
32540551	9	31	theme	vesicle	1553:1559	arg1	compositions					1561:1572	vesicle compositions	1553:1572	vesicle compositions	1553:1572	The outcome of the work is a defined set of conditions under which investigators can induce OMVs to fuse and make various combinations of vesicle compositions.
32540551	5	32	theme	fusion	589:594	arg1	kinetics					596:603	Vesicle aggregation and fusion kinetics	565:603	kinetics	596:603	Vesicle aggregation and fusion kinetics were investigated for OMVs isolated from native E. coli (Nissle 1917) and lipopolysaccharide (LPS) modified E. coli (ClearColi) strains to evaluate the effect of lipid type on vesicle aggregation and fusion.
32540551	2	33	theme	populations	310:320	arg1	fusion					296:301	induced fusion	288:301	induced fusion of OMV populations	288:320	The diversity of exogenous antigens delivered by OMVs can be enhanced by induced fusion of OMV populations.
32540551	1	34	theme	delivery	196:203	arg1	vesicles					158:165	Recombinantly engineered bacterial outer membrane vesicles	108:165	Recombinantly engineered bacterial outer membrane vesicles (OMVs)	108:172	Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles.
32540551	1	34	theme	delivery	196:203	arg1	vehicles					205:212	promising vaccine delivery vehicles	178:212	promising vaccine delivery vehicles	178:212	Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles.
32540551	3	35	theme	fusion	363:368	arg1	reports					344:350	no reports	341:350	no reports of induced fusion of bacterial OMVs	341:386	To date there are no reports of induced fusion of bacterial OMVs.
32540551	4	36	theme	stability	548:556	arg1	model					558:562	the Derjaguin-Landau-Verwey-Overbeek (DLVO) colloidal stability model	494:562	the Derjaguin-Landau-Verwey-Overbeek (DLVO) colloidal stability model	494:562	Here we measure the pH and salt-induced aggregation and fusion of OMVs and analyze the processes against the Derjaguin-Landau-Verwey-Overbeek (DLVO) colloidal stability model.
32540551	2	37	theme	OMV	306:308	arg1	populations					310:320	OMV populations	306:320	OMV populations	306:320	The diversity of exogenous antigens delivered by OMVs can be enhanced by induced fusion of OMV populations.
32540551	6	38	theme	efficiency	939:948	arg1	%					956:956	a calculated fusion efficiency of ~25%	919:956	a calculated fusion efficiency of ~25% (i.e. ~1/4 of collision events lead to fusion)	919:1003	Electrolytes and low pHs induced OMV aggregation for both native and modified LPS constructs, approaching a calculated fusion efficiency of ~25% (i.e. ~1/4 of collision events lead to fusion).
32540551	6	39	theme	OMV	846:848	arg1	aggregation					850:860	OMV aggregation	846:860	OMV aggregation for both native and modified LPS constructs	846:904	Electrolytes and low pHs induced OMV aggregation for both native and modified LPS constructs, approaching a calculated fusion efficiency of ~25% (i.e. ~1/4 of collision events lead to fusion).
32540551	8	40	theme	DLVO-predicted	1335:1348	arg1	concentrations					1371:1384	DLVO-predicted critical coagulation concentrations	1335:1384	DLVO-predicted critical coagulation concentrations with monovalent counterions	1335:1412	The lipid composition of the OMVs from Nissle negatively impacted fusion in the presence of electrolytes, causing higher deviations from DLVO-predicted critical coagulation concentrations with monovalent counterions.
32540551	9	41	theme	defined	1444:1450	arg1	conditions					1459:1468	conditions	1459:1468	conditions under which investigators can induce OMVs to fuse and make various combinations of vesicle compositions	1459:1572	The outcome of the work is a defined set of conditions under which investigators can induce OMVs to fuse and make various combinations of vesicle compositions.
32540551	9	41	theme	defined	1444:1450	arg1	set					1452:1454	a defined set	1442:1454	a defined set of conditions under which investigators can induce OMVs to fuse and make various combinations of vesicle compositions	1442:1572	The outcome of the work is a defined set of conditions under which investigators can induce OMVs to fuse and make various combinations of vesicle compositions.
32540551	9	41	theme	defined	1444:1450	arg1	outcome					1419:1425	The outcome	1415:1425	The outcome of the work	1415:1437	The outcome of the work is a defined set of conditions under which investigators can induce OMVs to fuse and make various combinations of vesicle compositions.
32540551	0	42	theme	bacterial	34:42	arg1	vesicles					59:66	bacterial outer membrane vesicles	34:66	bacterial outer membrane vesicles	34:66	Induced fusion and aggregation of bacterial outer membrane vesicles: Experimental and theoretical analysis.
32540551	6	43	theme	fusion	932:937	arg1	%					956:956	a calculated fusion efficiency of ~25%	919:956	a calculated fusion efficiency of ~25% (i.e. ~1/4 of collision events lead to fusion)	919:1003	Electrolytes and low pHs induced OMV aggregation for both native and modified LPS constructs, approaching a calculated fusion efficiency of ~25% (i.e. ~1/4 of collision events lead to fusion).
32540551	5	44	theme	modified	704:711	arg1	strains					733:739	modified E. coli (ClearColi) strains	704:739	modified E. coli (ClearColi) strains	704:739	Vesicle aggregation and fusion kinetics were investigated for OMVs isolated from native E. coli (Nissle 1917) and lipopolysaccharide (LPS) modified E. coli (ClearColi) strains to evaluate the effect of lipid type on vesicle aggregation and fusion.
32540551	7	45	theme	pH	1127:1128	arg1	combination					1108:1118	a combination	1106:1118	a combination of low pH	1106:1128	However, high fusion efficiency was achieved for Nissle OMVs solely with decreased pH as opposed to a combination of low pH and increased divalent counterion concentration for ClearColi OMVs.
32540551	8	46	theme	lipid	1202:1206	arg1	composition					1208:1218	The lipid composition	1198:1218	The lipid composition of the OMVs from Nissle	1198:1242	The lipid composition of the OMVs from Nissle negatively impacted fusion in the presence of electrolytes, causing higher deviations from DLVO-predicted critical coagulation concentrations with monovalent counterions.
32540551	6	47	theme	calculated	921:930	arg1	%					956:956	a calculated fusion efficiency of ~25%	919:956	a calculated fusion efficiency of ~25% (i.e. ~1/4 of collision events lead to fusion)	919:1003	Electrolytes and low pHs induced OMV aggregation for both native and modified LPS constructs, approaching a calculated fusion efficiency of ~25% (i.e. ~1/4 of collision events lead to fusion).
32540551	7	48	theme	fusion	1020:1025	arg1	efficiency					1027:1036	high fusion efficiency	1015:1036	high fusion efficiency	1015:1036	However, high fusion efficiency was achieved for Nissle OMVs solely with decreased pH as opposed to a combination of low pH and increased divalent counterion concentration for ClearColi OMVs.
32540551	2	49	theme	antigens	242:249	arg1	diversity					219:227	The diversity	215:227	The diversity of exogenous antigens delivered by OMVs	215:267	The diversity of exogenous antigens delivered by OMVs can be enhanced by induced fusion of OMV populations.
32540551	8	50	from	composition	1208:1218	arg1	Nissle					1237:1242	Nissle	1237:1242	Nissle	1237:1242	The lipid composition of the OMVs from Nissle negatively impacted fusion in the presence of electrolytes, causing higher deviations from DLVO-predicted critical coagulation concentrations with monovalent counterions.
32540551	8	51	theme	OMVs	1227:1230	arg1	composition					1208:1218	The lipid composition	1198:1218	The lipid composition of the OMVs from Nissle	1198:1242	The lipid composition of the OMVs from Nissle negatively impacted fusion in the presence of electrolytes, causing higher deviations from DLVO-predicted critical coagulation concentrations with monovalent counterions.
32540551	0	52	theme	membrane	50:57	arg1	vesicles					59:66	bacterial outer membrane vesicles	34:66	bacterial outer membrane vesicles	34:66	Induced fusion and aggregation of bacterial outer membrane vesicles: Experimental and theoretical analysis.
32540551	7	53	theme	divalent	1144:1151	arg1	concentration					1164:1176	divalent counterion concentration	1144:1176	divalent counterion concentration for ClearColi OMVs	1144:1195	However, high fusion efficiency was achieved for Nissle OMVs solely with decreased pH as opposed to a combination of low pH and increased divalent counterion concentration for ClearColi OMVs.
32540551	6	54	theme	low	830:832	arg1	pHs					834:836	low pHs	830:836	low pHs	830:836	Electrolytes and low pHs induced OMV aggregation for both native and modified LPS constructs, approaching a calculated fusion efficiency of ~25% (i.e. ~1/4 of collision events lead to fusion).
32540551	2	55	theme	exogenous	232:240	arg1	antigens					242:249	exogenous antigens	232:249	exogenous antigens delivered by OMVs	232:267	The diversity of exogenous antigens delivered by OMVs can be enhanced by induced fusion of OMV populations.
32540551	1	56	theme	engineered	122:131	arg1	OMVs					168:171	OMVs	168:171	OMVs	168:171	Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles.
32540551	1	56	theme	engineered	122:131	arg1	vehicles					205:212	promising vaccine delivery vehicles	178:212	promising vaccine delivery vehicles	178:212	Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles.
32540551	1	56	theme	engineered	122:131	arg1	vesicles					158:165	Recombinantly engineered bacterial outer membrane vesicles	108:165	Recombinantly engineered bacterial outer membrane vesicles (OMVs)	108:172	Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles.
32540551	0	57	theme	outer	44:48	arg1	vesicles					59:66	bacterial outer membrane vesicles	34:66	bacterial outer membrane vesicles	34:66	Induced fusion and aggregation of bacterial outer membrane vesicles: Experimental and theoretical analysis.
32540551	4	58	theme	colloidal	538:546	arg1	model					558:562	the Derjaguin-Landau-Verwey-Overbeek (DLVO) colloidal stability model	494:562	the Derjaguin-Landau-Verwey-Overbeek (DLVO) colloidal stability model	494:562	Here we measure the pH and salt-induced aggregation and fusion of OMVs and analyze the processes against the Derjaguin-Landau-Verwey-Overbeek (DLVO) colloidal stability model.
32540551	6	59	theme	modified	882:889	arg1	constructs					895:904	both native and modified LPS constructs	866:904	both native and modified LPS constructs	866:904	Electrolytes and low pHs induced OMV aggregation for both native and modified LPS constructs, approaching a calculated fusion efficiency of ~25% (i.e. ~1/4 of collision events lead to fusion).
32540551	7	60	theme	counterion	1153:1162	arg1	concentration					1164:1176	divalent counterion concentration	1144:1176	divalent counterion concentration for ClearColi OMVs	1144:1195	However, high fusion efficiency was achieved for Nissle OMVs solely with decreased pH as opposed to a combination of low pH and increased divalent counterion concentration for ClearColi OMVs.
32540551	1	61	theme	bacterial	133:141	arg1	OMVs					168:171	OMVs	168:171	OMVs	168:171	Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles.
32540551	1	61	theme	bacterial	133:141	arg1	vehicles					205:212	promising vaccine delivery vehicles	178:212	promising vaccine delivery vehicles	178:212	Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles.
32540551	1	61	theme	bacterial	133:141	arg1	vesicles					158:165	Recombinantly engineered bacterial outer membrane vesicles	108:165	Recombinantly engineered bacterial outer membrane vesicles (OMVs)	108:172	Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles.
32540551	5	62	theme	Vesicle	565:571	arg1	aggregation					573:583	Vesicle aggregation and fusion kinetics	565:603	aggregation	573:583	Vesicle aggregation and fusion kinetics were investigated for OMVs isolated from native E. coli (Nissle 1917) and lipopolysaccharide (LPS) modified E. coli (ClearColi) strains to evaluate the effect of lipid type on vesicle aggregation and fusion.
32540551	1	63	theme	outer	143:147	arg1	OMVs					168:171	OMVs	168:171	OMVs	168:171	Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles.
32540551	1	63	theme	outer	143:147	arg1	vehicles					205:212	promising vaccine delivery vehicles	178:212	promising vaccine delivery vehicles	178:212	Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles.
32540551	1	63	theme	outer	143:147	arg1	vesicles					158:165	Recombinantly engineered bacterial outer membrane vesicles	108:165	Recombinantly engineered bacterial outer membrane vesicles (OMVs)	108:172	Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles.
32540551	8	64	theme	critical	1350:1357	arg1	concentrations					1371:1384	DLVO-predicted critical coagulation concentrations	1335:1384	DLVO-predicted critical coagulation concentrations with monovalent counterions	1335:1412	The lipid composition of the OMVs from Nissle negatively impacted fusion in the presence of electrolytes, causing higher deviations from DLVO-predicted critical coagulation concentrations with monovalent counterions.
32540551	5	65	dep	coli	656:659	arg1	strains					733:739	modified E. coli (ClearColi) strains	704:739	modified E. coli (ClearColi) strains	704:739	Vesicle aggregation and fusion kinetics were investigated for OMVs isolated from native E. coli (Nissle 1917) and lipopolysaccharide (LPS) modified E. coli (ClearColi) strains to evaluate the effect of lipid type on vesicle aggregation and fusion.
32540551	4	66	theme	salt-induced	416:427	arg1	aggregation					429:439	salt-induced aggregation	416:439	salt-induced aggregation	416:439	Here we measure the pH and salt-induced aggregation and fusion of OMVs and analyze the processes against the Derjaguin-Landau-Verwey-Overbeek (DLVO) colloidal stability model.
32540551	1	67	theme	membrane	149:156	arg1	OMVs					168:171	OMVs	168:171	OMVs	168:171	Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles.
32540551	1	67	theme	membrane	149:156	arg1	vehicles					205:212	promising vaccine delivery vehicles	178:212	promising vaccine delivery vehicles	178:212	Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles.
32540551	1	67	theme	membrane	149:156	arg1	vesicles					158:165	Recombinantly engineered bacterial outer membrane vesicles	108:165	Recombinantly engineered bacterial outer membrane vesicles (OMVs)	108:172	Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles.
32540551	0	68	dep	fusion	8:13	arg1	analysis					98:105	Experimental and theoretical analysis	69:105	Induced fusion and aggregation of bacterial outer membrane vesicles: Experimental and theoretical analysis.	0:106	Induced fusion and aggregation of bacterial outer membrane vesicles: Experimental and theoretical analysis.
32540551	4	69	dep	pH	409:410	arg1	the					405:407	the	405:407	the	405:407	Here we measure the pH and salt-induced aggregation and fusion of OMVs and analyze the processes against the Derjaguin-Landau-Verwey-Overbeek (DLVO) colloidal stability model.
32540551	0	70	theme	vesicles	59:66	arg1	aggregation					19:29	aggregation	19:29	aggregation	19:29	Induced fusion and aggregation of bacterial outer membrane vesicles: Experimental and theoretical analysis.
32540551	0	70	theme	vesicles	59:66	arg1	fusion					8:13	fusion	8:13	fusion	8:13	Induced fusion and aggregation of bacterial outer membrane vesicles: Experimental and theoretical analysis.
32540551	5	71	from	effect	757:762	arg1	fusion					805:810	fusion	805:810	fusion	805:810	Vesicle aggregation and fusion kinetics were investigated for OMVs isolated from native E. coli (Nissle 1917) and lipopolysaccharide (LPS) modified E. coli (ClearColi) strains to evaluate the effect of lipid type on vesicle aggregation and fusion.
32540551	5	71	from	effect	757:762	arg1	aggregation					789:799	vesicle aggregation	781:799	vesicle aggregation	781:799	Vesicle aggregation and fusion kinetics were investigated for OMVs isolated from native E. coli (Nissle 1917) and lipopolysaccharide (LPS) modified E. coli (ClearColi) strains to evaluate the effect of lipid type on vesicle aggregation and fusion.
32540551	7	72	theme	high	1015:1018	arg1	efficiency					1027:1036	high fusion efficiency	1015:1036	high fusion efficiency	1015:1036	However, high fusion efficiency was achieved for Nissle OMVs solely with decreased pH as opposed to a combination of low pH and increased divalent counterion concentration for ClearColi OMVs.
32540551	7	73	theme	low	1123:1125	arg1	pH					1127:1128	low pH	1123:1128	low pH	1123:1128	However, high fusion efficiency was achieved for Nissle OMVs solely with decreased pH as opposed to a combination of low pH and increased divalent counterion concentration for ClearColi OMVs.
32540551	3	74	theme	induced	355:361	arg1	fusion					363:368	induced fusion	355:368	induced fusion of bacterial OMVs	355:386	To date there are no reports of induced fusion of bacterial OMVs.
34443588	11	0	theme	Alg/BCNs-CS-GT	2183:2196	arg1	scaffolds					2208:2216	the Alg/BCNs-CS-GT composite scaffolds	2179:2216	the Alg/BCNs-CS-GT composite scaffolds	2179:2216	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	11	1	theme	good	2155:2158	arg1	cytocompatibility					2160:2176	good cytocompatibility	2155:2176	good cytocompatibility	2155:2176	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	2	2	from	gelation	602:609	arg1	matrix					585:590	the alginate matrix	572:590	the alginate matrix	572:590	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	1	3	theme	uncontrolled	267:278	arg1	degradation					280:290	uncontrolled degradation	267:290	uncontrolled degradation	267:290	It is common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold.
34443588	11	4	theme	engineering	2287:2297	arg1	field					2299:2303	the bone tissue engineering field	2271:2303	the bone tissue engineering field	2271:2303	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	3	5	theme	contents	779:786	arg1	Meanwhile					746:754	Meanwhile	746:754	Meanwhile	746:754	Meanwhile, the effect of various contents of BCNs on the scaffold morphology, porosity, mechanical properties, and swelling and degradation behavior was investigated.
34443588	3	5	theme	contents	779:786	arg1	effect					761:766	the effect	757:766	the effect of various contents of BCNs on the scaffold morphology, porosity, mechanical properties, and swelling and degradation behavior	757:893	Meanwhile, the effect of various contents of BCNs on the scaffold morphology, porosity, mechanical properties, and swelling and degradation behavior was investigated.
34443588	11	6	theme	well-developed	2071:2084	arg1	structure					2091:2099	well-developed pore structure	2071:2099	well-developed pore structure	2071:2099	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	11	7	theme	ideal	2253:2257	arg1	potential					2236:2244	great potential	2230:2244	great potential	2230:2244	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	11	7	theme	ideal	2253:2257	arg1	scaffold					2259:2266	the ideal scaffold	2249:2266	the ideal scaffold in the bone tissue engineering field	2249:2303	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	5	8	from	increase	1079:1086	arg1	content					1096:1102	BCNs content	1091:1102	BCNs content	1091:1102	With the increase in BCNs content, the pore size of Alg/BCNs-CS-GT composite scaffolds was gradually reduced from 200 μm to 70 μm.
34443588	8	9	dep	in	1467:1468	arg1	vitro					1470:1474	vitro	1470:1474	vitro	1470:1474	Furthermore, the in vitro cytotoxicity studies indicated that the porous fiber network of BCNs could fully mimic the extracellular matrix structure, which promoted the adhesion and spreading of MG63 cells and MC3T3-E1 cells on the Alg/BCNs-CS-GT composite scaffolds.
34443588	10	10	theme	BCNs	1873:1876	arg1	effect					1863:1868	the effect	1859:1868	the effect of BCNs on the cytocompatibility of composite scaffolds	1859:1924	Based on the effect of BCNs on the cytocompatibility of composite scaffolds, the optimum BCNs content for the Alg/BCNs-CS-GT composite scaffolds was 0.2% (w/v).
34443588	4	11	theme	experimental	917:928	arg1	results					930:936	The experimental results	913:936	The experimental results	913:936	The experimental results showed that the fabricated Alg/BCNs-CS-GT composite scaffolds exhibited regular 3D morphologies and well-developed pore structures.
34443588	2	12	theme	alginate	576:583	arg1	matrix					585:590	the alginate matrix	572:590	the alginate matrix	572:590	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	9	13	theme	composite	1783:1791	arg1	scaffolds					1793:1801	composite scaffolds	1783:1801	composite scaffolds	1783:1801	In addition, these cells could grow in the 3D-porous structure of composite scaffolds, which exhibited good proliferative viability.
34443588	3	14	theme	BCNs	791:794	arg1	contents					779:786	various contents	771:786	various contents of BCNs	771:794	Meanwhile, the effect of various contents of BCNs on the scaffold morphology, porosity, mechanical properties, and swelling and degradation behavior was investigated.
34443588	4	15	theme	3D	1018:1019	arg1	morphologies					1021:1032	regular 3D morphologies	1010:1032	regular 3D morphologies	1010:1032	The experimental results showed that the fabricated Alg/BCNs-CS-GT composite scaffolds exhibited regular 3D morphologies and well-developed pore structures.
34443588	1	16	theme	recognition	225:235	arg1	sites					237:241	cell recognition sites	220:241	cell recognition sites	220:241	It is common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold.
34443588	4	17	theme	fabricated	954:963	arg1	scaffolds					990:998	the fabricated Alg/BCNs-CS-GT composite scaffolds	950:998	the fabricated Alg/BCNs-CS-GT composite scaffolds	950:998	The experimental results showed that the fabricated Alg/BCNs-CS-GT composite scaffolds exhibited regular 3D morphologies and well-developed pore structures.
34443588	9	18	theme	proliferative	1825:1837	arg1	viability					1839:1847	good proliferative viability	1820:1847	good proliferative viability	1820:1847	In addition, these cells could grow in the 3D-porous structure of composite scaffolds, which exhibited good proliferative viability.
34443588	3	19	from	effect	761:766	arg1	porosity					824:831	porosity	824:831	porosity	824:831	Meanwhile, the effect of various contents of BCNs on the scaffold morphology, porosity, mechanical properties, and swelling and degradation behavior was investigated.
34443588	3	19	from	effect	761:766	arg1	properties					845:854	mechanical properties	834:854	mechanical properties	834:854	Meanwhile, the effect of various contents of BCNs on the scaffold morphology, porosity, mechanical properties, and swelling and degradation behavior was investigated.
34443588	3	19	from	effect	761:766	arg1	morphology					812:821	the scaffold morphology	799:821	the scaffold morphology	799:821	Meanwhile, the effect of various contents of BCNs on the scaffold morphology, porosity, mechanical properties, and swelling and degradation behavior was investigated.
34443588	3	19	from	effect	761:766	arg1	behavior					886:893	swelling and degradation behavior	861:893	swelling and degradation behavior	861:893	Meanwhile, the effect of various contents of BCNs on the scaffold morphology, porosity, mechanical properties, and swelling and degradation behavior was investigated.
34443588	4	20	theme	composite	980:988	arg1	scaffolds					990:998	the fabricated Alg/BCNs-CS-GT composite scaffolds	950:998	the fabricated Alg/BCNs-CS-GT composite scaffolds	950:998	The experimental results showed that the fabricated Alg/BCNs-CS-GT composite scaffolds exhibited regular 3D morphologies and well-developed pore structures.
34443588	2	21	from	incorporation	547:559	arg1	matrix					585:590	the alginate matrix	572:590	the alginate matrix	572:590	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	6	22	theme	hydrogen	1289:1296	arg1	bond					1298:1301	the intermolecular hydrogen bond	1270:1301	the intermolecular hydrogen bond with alginate	1270:1315	Furthermore, BCNs were fully embedded in the alginate matrix through the intermolecular hydrogen bond with alginate.
34443588	5	23	theme	composite	1137:1145	arg1	scaffolds					1147:1155	Alg/BCNs-CS-GT composite scaffolds	1122:1155	Alg/BCNs-CS-GT composite scaffolds	1122:1155	With the increase in BCNs content, the pore size of Alg/BCNs-CS-GT composite scaffolds was gradually reduced from 200 μm to 70 μm.
34443588	11	24	theme	regular	2048:2054	arg1	morphology					2059:2068	regular 3D morphology	2048:2068	regular 3D morphology	2048:2068	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	6	25	with	bond	1298:1301	arg1	alginate					1308:1315	alginate	1308:1315	alginate	1308:1315	Furthermore, BCNs were fully embedded in the alginate matrix through the intermolecular hydrogen bond with alginate.
34443588	8	26	theme	extracellular	1567:1579	arg1	structure					1588:1596	the extracellular matrix structure	1563:1596	the extracellular matrix structure	1563:1596	Furthermore, the in vitro cytotoxicity studies indicated that the porous fiber network of BCNs could fully mimic the extracellular matrix structure, which promoted the adhesion and spreading of MG63 cells and MC3T3-E1 cells on the Alg/BCNs-CS-GT composite scaffolds.
34443588	7	27	theme	composite	1429:1437	arg1	scaffolds					1439:1447	the Alg/BCNs-CS-GT composite scaffolds	1410:1447	the Alg/BCNs-CS-GT composite scaffolds	1410:1447	Moreover, the addition of BCNs could effectively control the swelling and biodegradation of the Alg/BCNs-CS-GT composite scaffolds.
34443588	3	28	theme	mechanical	834:843	arg1	properties					845:854	mechanical properties	834:854	mechanical properties	834:854	Meanwhile, the effect of various contents of BCNs on the scaffold morphology, porosity, mechanical properties, and swelling and degradation behavior was investigated.
34443588	0	29	theme	Scaffolds	99:107	arg1	Evaluation					16:25	Evaluation	16:25	Evaluation	16:25	Fabrication and Evaluation of Alginate/Bacterial Cellulose Nanocrystals-Chitosan-Gelatin Composite Scaffolds.
34443588	0	29	theme	Scaffolds	99:107	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and Evaluation of Alginate/Bacterial Cellulose Nanocrystals-Chitosan-Gelatin Composite Scaffolds.
34443588	10	30	theme	scaffolds	1916:1924	arg1	cytocompatibility					1885:1901	the cytocompatibility	1881:1901	the cytocompatibility of composite scaffolds	1881:1924	Based on the effect of BCNs on the cytocompatibility of composite scaffolds, the optimum BCNs content for the Alg/BCNs-CS-GT composite scaffolds was 0.2% (w/v).
34443588	11	31	theme	tissue	2280:2285	arg1	field					2299:2303	the bone tissue engineering field	2271:2303	the bone tissue engineering field	2271:2303	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	2	32	theme	internal	593:600	arg1	gelation					602:609	internal gelation	593:609	internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex	593:674	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	3	33	theme	degradation	874:884	arg1	behavior					886:893	swelling and degradation behavior	861:893	swelling and degradation behavior	861:893	Meanwhile, the effect of various contents of BCNs on the scaffold morphology, porosity, mechanical properties, and swelling and degradation behavior was investigated.
34443588	1	34	theme	ideal	342:346	arg1	scaffold					348:355	the ideal scaffold	338:355	the ideal scaffold	338:355	It is common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold.
34443588	1	35	theme	weak	184:187	arg1	strength					200:207	weak mechanical strength	184:207	weak mechanical strength	184:207	It is common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold.
34443588	3	36	theme	swelling	861:868	arg1	behavior					886:893	swelling and degradation behavior	861:893	swelling and degradation behavior	861:893	Meanwhile, the effect of various contents of BCNs on the scaffold morphology, porosity, mechanical properties, and swelling and degradation behavior was investigated.
34443588	4	37	theme	pore	1053:1056	arg1	structures					1058:1067	well-developed pore structures	1038:1067	well-developed pore structures	1038:1067	The experimental results showed that the fabricated Alg/BCNs-CS-GT composite scaffolds exhibited regular 3D morphologies and well-developed pore structures.
34443588	0	38	theme	Cellulose	49:57	arg1	Scaffolds					99:107	Alginate/Bacterial Cellulose Nanocrystals-Chitosan-Gelatin Composite Scaffolds	30:107	Alginate/Bacterial Cellulose Nanocrystals-Chitosan-Gelatin Composite Scaffolds	30:107	Fabrication and Evaluation of Alginate/Bacterial Cellulose Nanocrystals-Chitosan-Gelatin Composite Scaffolds.
34443588	11	39	theme	swelling	2113:2120	arg1	behavior					2141:2148	controlled swelling and biodegradation behavior	2102:2148	controlled swelling and biodegradation behavior	2102:2148	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	7	40	theme	Alg/BCNs-CS-GT	1414:1427	arg1	scaffolds					1439:1447	the Alg/BCNs-CS-GT composite scaffolds	1410:1447	the Alg/BCNs-CS-GT composite scaffolds	1410:1447	Moreover, the addition of BCNs could effectively control the swelling and biodegradation of the Alg/BCNs-CS-GT composite scaffolds.
34443588	2	41	theme	polyelectrolytes	728:743	arg1	gelation					602:609	internal gelation	593:609	internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex	593:674	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	2	41	theme	polyelectrolytes	728:743	arg1	assembly					716:723	layer-by-layer (LBL) electrostatic assembly	681:723	layer-by-layer (LBL) electrostatic assembly of polyelectrolytes	681:743	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	2	41	theme	polyelectrolytes	728:743	arg1	incorporation					547:559	the incorporation	543:559	the incorporation of BCNs in the alginate matrix	543:590	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	2	42	from	BCNs	564:567	arg1	matrix					585:590	the alginate matrix	572:590	the alginate matrix	572:590	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	2	43	theme	cellulose	430:438	arg1	scaffolds					497:505	alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds	411:505	alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes	411:743	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	1	44	theme	pure	138:141	arg1	hydrogel					152:159	pure alginate hydrogel	138:159	pure alginate hydrogel	138:159	It is common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold.
34443588	8	45	theme	cells	1649:1653	arg1	adhesion					1618:1625	adhesion	1618:1625	adhesion	1618:1625	Furthermore, the in vitro cytotoxicity studies indicated that the porous fiber network of BCNs could fully mimic the extracellular matrix structure, which promoted the adhesion and spreading of MG63 cells and MC3T3-E1 cells on the Alg/BCNs-CS-GT composite scaffolds.
34443588	8	45	theme	cells	1649:1653	arg1	spreading					1631:1639	spreading	1631:1639	spreading	1631:1639	Furthermore, the in vitro cytotoxicity studies indicated that the porous fiber network of BCNs could fully mimic the extracellular matrix structure, which promoted the adhesion and spreading of MG63 cells and MC3T3-E1 cells on the Alg/BCNs-CS-GT composite scaffolds.
34443588	0	46	theme	Alginate/Bacterial	30:47	arg1	Scaffolds					99:107	Alginate/Bacterial Cellulose Nanocrystals-Chitosan-Gelatin Composite Scaffolds	30:107	Alginate/Bacterial Cellulose Nanocrystals-Chitosan-Gelatin Composite Scaffolds	30:107	Fabrication and Evaluation of Alginate/Bacterial Cellulose Nanocrystals-Chitosan-Gelatin Composite Scaffolds.
34443588	8	47	theme	MC3T3-E1	1659:1666	arg1	cells					1668:1672	MC3T3-E1 cells	1659:1672	MC3T3-E1 cells	1659:1672	Furthermore, the in vitro cytotoxicity studies indicated that the porous fiber network of BCNs could fully mimic the extracellular matrix structure, which promoted the adhesion and spreading of MG63 cells and MC3T3-E1 cells on the Alg/BCNs-CS-GT composite scaffolds.
34443588	10	48	theme	BCNs	1939:1942	arg1	content					1944:1950	the optimum BCNs content	1927:1950	the optimum BCNs content for the Alg/BCNs-CS-GT composite scaffolds	1927:1993	Based on the effect of BCNs on the cytocompatibility of composite scaffolds, the optimum BCNs content for the Alg/BCNs-CS-GT composite scaffolds was 0.2% (w/v).
34443588	10	48	theme	BCNs	1939:1942	arg1	%					2002:2002	0.2%	1999:2002	0.2% (w/v)	1999:2008	Based on the effect of BCNs on the cytocompatibility of composite scaffolds, the optimum BCNs content for the Alg/BCNs-CS-GT composite scaffolds was 0.2% (w/v).
34443588	0	49	theme	Nanocrystals-Chitosan-Gelatin	59:87	arg1	Scaffolds					99:107	Alginate/Bacterial Cellulose Nanocrystals-Chitosan-Gelatin Composite Scaffolds	30:107	Alginate/Bacterial Cellulose Nanocrystals-Chitosan-Gelatin Composite Scaffolds	30:107	Fabrication and Evaluation of Alginate/Bacterial Cellulose Nanocrystals-Chitosan-Gelatin Composite Scaffolds.
34443588	11	50	theme	great	2230:2234	arg1	potential					2236:2244	great potential	2230:2244	great potential	2230:2244	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	11	50	theme	great	2230:2234	arg1	scaffold					2259:2266	the ideal scaffold	2249:2266	the ideal scaffold in the bone tissue engineering field	2249:2303	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	8	51	theme	porous	1516:1521	arg1	network					1529:1535	the porous fiber network	1512:1535	the porous fiber network of BCNs	1512:1543	Furthermore, the in vitro cytotoxicity studies indicated that the porous fiber network of BCNs could fully mimic the extracellular matrix structure, which promoted the adhesion and spreading of MG63 cells and MC3T3-E1 cells on the Alg/BCNs-CS-GT composite scaffolds.
34443588	1	52	theme	sites	237:241	arg1	swelling					254:261	extensive swelling	244:261	extensive swelling	244:261	It is common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold.
34443588	1	52	theme	sites	237:241	arg1	degradation					280:290	uncontrolled degradation	267:290	uncontrolled degradation	267:290	It is common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold.
34443588	1	52	theme	sites	237:241	arg1	strength					200:207	weak mechanical strength	184:207	weak mechanical strength	184:207	It is common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold.
34443588	1	52	theme	sites	237:241	arg1	lack					212:215	a lack	210:215	a lack of cell recognition sites	210:241	It is common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold.
34443588	7	53	theme	BCNs	1344:1347	arg1	addition					1332:1339	the addition	1328:1339	the addition of BCNs	1328:1347	Moreover, the addition of BCNs could effectively control the swelling and biodegradation of the Alg/BCNs-CS-GT composite scaffolds.
34443588	10	54	theme	Alg/BCNs-CS-GT	1960:1973	arg1	scaffolds					1985:1993	the Alg/BCNs-CS-GT composite scaffolds	1956:1993	the Alg/BCNs-CS-GT composite scaffolds	1956:1993	Based on the effect of BCNs on the cytocompatibility of composite scaffolds, the optimum BCNs content for the Alg/BCNs-CS-GT composite scaffolds was 0.2% (w/v).
34443588	1	55	theme	extensive	244:252	arg1	swelling					254:261	extensive swelling	244:261	extensive swelling	244:261	It is common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold.
34443588	8	56	from	adhesion	1618:1625	arg1	scaffolds					1706:1714	the Alg/BCNs-CS-GT composite scaffolds	1677:1714	the Alg/BCNs-CS-GT composite scaffolds	1677:1714	Furthermore, the in vitro cytotoxicity studies indicated that the porous fiber network of BCNs could fully mimic the extracellular matrix structure, which promoted the adhesion and spreading of MG63 cells and MC3T3-E1 cells on the Alg/BCNs-CS-GT composite scaffolds.
34443588	11	57	theme	composite	2198:2206	arg1	scaffolds					2208:2216	the Alg/BCNs-CS-GT composite scaffolds	2179:2216	the Alg/BCNs-CS-GT composite scaffolds	2179:2216	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	2	58	from	matrix	585:590	arg1	gelation					602:609	internal gelation	593:609	internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex	593:674	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	2	58	from	matrix	585:590	arg1	assembly					716:723	layer-by-layer (LBL) electrostatic assembly	681:723	layer-by-layer (LBL) electrostatic assembly of polyelectrolytes	681:743	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	2	58	from	matrix	585:590	arg1	incorporation					547:559	the incorporation	543:559	the incorporation of BCNs in the alginate matrix	543:590	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	11	59	theme	biodegradation	2126:2139	arg1	behavior					2141:2148	controlled swelling and biodegradation behavior	2102:2148	controlled swelling and biodegradation behavior	2102:2148	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	2	60	theme	HAP-GDL	659:665	arg1	complex					668:674	the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex	619:674	the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex	619:674	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	8	61	dep	adhesion	1618:1625	arg1	the					1614:1616	the	1614:1616	the	1614:1616	Furthermore, the in vitro cytotoxicity studies indicated that the porous fiber network of BCNs could fully mimic the extracellular matrix structure, which promoted the adhesion and spreading of MG63 cells and MC3T3-E1 cells on the Alg/BCNs-CS-GT composite scaffolds.
34443588	2	62	theme	hydroxyapatite-d-glucono-δ-lactone	623:656	arg1	complex					668:674	the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex	619:674	the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex	619:674	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	6	63	theme	alginate	1246:1253	arg1	matrix					1255:1260	the alginate matrix	1242:1260	the alginate matrix through the intermolecular hydrogen bond with alginate	1242:1315	Furthermore, BCNs were fully embedded in the alginate matrix through the intermolecular hydrogen bond with alginate.
34443588	1	64	contain	have	179:182	arg2	degradation					280:290	uncontrolled degradation	267:290	uncontrolled degradation	267:290	It is common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold.
34443588	1	64	contain	have	179:182	arg2	swelling					254:261	extensive swelling	244:261	extensive swelling	244:261	It is common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold.
34443588	1	64	contain	have	179:182	arg1	hydrogel					152:159	pure alginate hydrogel	138:159	pure alginate hydrogel	138:159	It is common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold.
34443588	1	64	contain	have	179:182	arg2	strength					200:207	weak mechanical strength	184:207	weak mechanical strength	184:207	It is common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold.
34443588	1	64	contain	have	179:182	arg2	lack					212:215	a lack	210:215	a lack of cell recognition sites	210:241	It is common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold.
34443588	11	65	theme	3D	2056:2057	arg1	morphology					2059:2068	regular 3D morphology	2048:2068	regular 3D morphology	2048:2068	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	1	66	theme	mechanical	189:198	arg1	strength					200:207	weak mechanical strength	184:207	weak mechanical strength	184:207	It is common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold.
34443588	9	67	theme	3D-porous	1760:1768	arg1	structure					1770:1778	the 3D-porous structure	1756:1778	the 3D-porous structure of composite scaffolds, which exhibited good proliferative viability	1756:1847	In addition, these cells could grow in the 3D-porous structure of composite scaffolds, which exhibited good proliferative viability.
34443588	3	68	theme	various	771:777	arg1	contents					779:786	various contents	771:786	various contents of BCNs	771:794	Meanwhile, the effect of various contents of BCNs on the scaffold morphology, porosity, mechanical properties, and swelling and degradation behavior was investigated.
34443588	3	69	theme	scaffold	803:810	arg1	morphology					812:821	the scaffold morphology	799:821	the scaffold morphology	799:821	Meanwhile, the effect of various contents of BCNs on the scaffold morphology, porosity, mechanical properties, and swelling and degradation behavior was investigated.
34443588	11	70	theme	pore	2086:2089	arg1	structure					2091:2099	well-developed pore structure	2071:2099	well-developed pore structure	2071:2099	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	11	71	theme	controlled	2102:2111	arg1	behavior					2141:2148	controlled swelling and biodegradation behavior	2102:2148	controlled swelling and biodegradation behavior	2102:2148	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	9	72	theme	scaffolds	1793:1801	arg1	structure					1770:1778	the 3D-porous structure	1756:1778	the 3D-porous structure of composite scaffolds, which exhibited good proliferative viability	1756:1847	In addition, these cells could grow in the 3D-porous structure of composite scaffolds, which exhibited good proliferative viability.
34443588	8	73	theme	BCNs	1540:1543	arg1	network					1529:1535	the porous fiber network	1512:1535	the porous fiber network of BCNs	1512:1543	Furthermore, the in vitro cytotoxicity studies indicated that the porous fiber network of BCNs could fully mimic the extracellular matrix structure, which promoted the adhesion and spreading of MG63 cells and MC3T3-E1 cells on the Alg/BCNs-CS-GT composite scaffolds.
34443588	10	74	theme	composite	1906:1914	arg1	scaffolds					1916:1924	composite scaffolds	1906:1924	composite scaffolds	1906:1924	Based on the effect of BCNs on the cytocompatibility of composite scaffolds, the optimum BCNs content for the Alg/BCNs-CS-GT composite scaffolds was 0.2% (w/v).
34443588	11	75	dep	merits	2032:2037	arg1	basis					2018:2022	basis	2018:2022	basis	2018:2022	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	11	75	dep	merits	2032:2037	arg1	the					2014:2016	the	2014:2016	the	2014:2016	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	1	76	theme	cell	220:223	arg1	sites					237:241	cell recognition sites	220:241	cell recognition sites	220:241	It is common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold.
34443588	4	77	theme	regular	1010:1016	arg1	morphologies					1021:1032	regular 3D morphologies	1010:1032	regular 3D morphologies	1010:1032	The experimental results showed that the fabricated Alg/BCNs-CS-GT composite scaffolds exhibited regular 3D morphologies and well-developed pore structures.
34443588	1	78	theme	alginate	143:150	arg1	hydrogel					152:159	pure alginate hydrogel	138:159	pure alginate hydrogel	138:159	It is common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold.
34443588	9	79	theme	good	1820:1823	arg1	viability					1839:1847	good proliferative viability	1820:1847	good proliferative viability	1820:1847	In addition, these cells could grow in the 3D-porous structure of composite scaffolds, which exhibited good proliferative viability.
34443588	5	80	theme	Alg/BCNs-CS-GT	1122:1135	arg1	scaffolds					1147:1155	Alg/BCNs-CS-GT composite scaffolds	1122:1155	Alg/BCNs-CS-GT composite scaffolds	1122:1155	With the increase in BCNs content, the pore size of Alg/BCNs-CS-GT composite scaffolds was gradually reduced from 200 μm to 70 μm.
34443588	10	81	theme	optimum	1931:1937	arg1	content					1944:1950	the optimum BCNs content	1927:1950	the optimum BCNs content for the Alg/BCNs-CS-GT composite scaffolds	1927:1993	Based on the effect of BCNs on the cytocompatibility of composite scaffolds, the optimum BCNs content for the Alg/BCNs-CS-GT composite scaffolds was 0.2% (w/v).
34443588	10	81	theme	optimum	1931:1937	arg1	%					2002:2002	0.2%	1999:2002	0.2% (w/v)	1999:2008	Based on the effect of BCNs on the cytocompatibility of composite scaffolds, the optimum BCNs content for the Alg/BCNs-CS-GT composite scaffolds was 0.2% (w/v).
34443588	4	82	theme	Alg/BCNs-CS-GT	965:978	arg1	scaffolds					990:998	the fabricated Alg/BCNs-CS-GT composite scaffolds	950:998	the fabricated Alg/BCNs-CS-GT composite scaffolds	950:998	The experimental results showed that the fabricated Alg/BCNs-CS-GT composite scaffolds exhibited regular 3D morphologies and well-developed pore structures.
34443588	8	83	theme	matrix	1581:1586	arg1	structure					1588:1596	the extracellular matrix structure	1563:1596	the extracellular matrix structure	1563:1596	Furthermore, the in vitro cytotoxicity studies indicated that the porous fiber network of BCNs could fully mimic the extracellular matrix structure, which promoted the adhesion and spreading of MG63 cells and MC3T3-E1 cells on the Alg/BCNs-CS-GT composite scaffolds.
34443588	6	84	theme	intermolecular	1274:1287	arg1	bond					1298:1301	the intermolecular hydrogen bond	1270:1301	the intermolecular hydrogen bond with alginate	1270:1315	Furthermore, BCNs were fully embedded in the alginate matrix through the intermolecular hydrogen bond with alginate.
34443588	5	85	theme	scaffolds	1147:1155	arg1	size					1114:1117	the pore size	1105:1117	the pore size of Alg/BCNs-CS-GT composite scaffolds	1105:1155	With the increase in BCNs content, the pore size of Alg/BCNs-CS-GT composite scaffolds was gradually reduced from 200 μm to 70 μm.
34443588	7	86	theme	scaffolds	1439:1447	arg1	swelling					1379:1386	swelling	1379:1386	swelling	1379:1386	Moreover, the addition of BCNs could effectively control the swelling and biodegradation of the Alg/BCNs-CS-GT composite scaffolds.
34443588	7	86	theme	scaffolds	1439:1447	arg1	biodegradation					1392:1405	biodegradation	1392:1405	biodegradation	1392:1405	Moreover, the addition of BCNs could effectively control the swelling and biodegradation of the Alg/BCNs-CS-GT composite scaffolds.
34443588	2	87	from	assembly	716:723	arg1	matrix					585:590	the alginate matrix	572:590	the alginate matrix	572:590	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	10	88	from	effect	1863:1868	arg1	cytocompatibility					1885:1901	the cytocompatibility	1881:1901	the cytocompatibility of composite scaffolds	1881:1924	Based on the effect of BCNs on the cytocompatibility of composite scaffolds, the optimum BCNs content for the Alg/BCNs-CS-GT composite scaffolds was 0.2% (w/v).
34443588	2	89	theme	combined	517:524	arg1	method					526:531	the combined method	513:531	the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes	513:743	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	2	90	theme	electrostatic	702:714	arg1	assembly					716:723	layer-by-layer (LBL) electrostatic assembly	681:723	layer-by-layer (LBL) electrostatic assembly of polyelectrolytes	681:743	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	1	91	theme	scaffold	348:355	arg1	demands					327:333	the demands	323:333	the demands of the ideal scaffold	323:355	It is common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold.
34443588	2	92	theme	BCNs	564:567	arg1	gelation					602:609	internal gelation	593:609	internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex	593:674	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	2	92	theme	BCNs	564:567	arg1	assembly					716:723	layer-by-layer (LBL) electrostatic assembly	681:723	layer-by-layer (LBL) electrostatic assembly of polyelectrolytes	681:743	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	2	92	theme	BCNs	564:567	arg1	incorporation					547:559	the incorporation	543:559	the incorporation of BCNs in the alginate matrix	543:590	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	5	93	theme	μm	1188:1189	arg1	μm					1197:1198	200 μm to 70 μm	1184:1198	200 μm to 70 μm	1184:1198	With the increase in BCNs content, the pore size of Alg/BCNs-CS-GT composite scaffolds was gradually reduced from 200 μm to 70 μm.
34443588	4	94	theme	well-developed	1038:1051	arg1	structures					1058:1067	well-developed pore structures	1038:1067	well-developed pore structures	1038:1067	The experimental results showed that the fabricated Alg/BCNs-CS-GT composite scaffolds exhibited regular 3D morphologies and well-developed pore structures.
34443588	6	95	from	matrix	1255:1260	arg1	embedded					1230:1237	embedded	1230:1237	embedded	1230:1237	Furthermore, BCNs were fully embedded in the alginate matrix through the intermolecular hydrogen bond with alginate.
34443588	1	96	theme	common	116:121	arg1	knowledge					123:131	common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold	116:355	common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold	116:355	It is common knowledge that pure alginate hydrogel is more likely to have weak mechanical strength, a lack of cell recognition sites, extensive swelling and uncontrolled degradation, and thus be unable to satisfy the demands of the ideal scaffold.
34443588	2	97	theme	Alg/BCNs-CS-GT	471:484	arg1	scaffolds					497:505	alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds	411:505	alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes	411:743	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	8	98	theme	MG63	1644:1647	arg1	cells					1649:1653	MG63 cells	1644:1653	MG63 cells	1644:1653	Furthermore, the in vitro cytotoxicity studies indicated that the porous fiber network of BCNs could fully mimic the extracellular matrix structure, which promoted the adhesion and spreading of MG63 cells and MC3T3-E1 cells on the Alg/BCNs-CS-GT composite scaffolds.
34443588	5	99	theme	BCNs	1091:1094	arg1	content					1096:1102	BCNs content	1091:1102	BCNs content	1091:1102	With the increase in BCNs content, the pore size of Alg/BCNs-CS-GT composite scaffolds was gradually reduced from 200 μm to 70 μm.
34443588	11	100	theme	good	2027:2030	arg1	behavior					2141:2148	controlled swelling and biodegradation behavior	2102:2148	controlled swelling and biodegradation behavior	2102:2148	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	11	100	theme	good	2027:2030	arg1	structure					2091:2099	well-developed pore structure	2071:2099	well-developed pore structure	2071:2099	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	11	100	theme	good	2027:2030	arg1	merits					2032:2037	good merits	2027:2037	good merits	2027:2037	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	11	100	theme	good	2027:2030	arg1	morphology					2059:2068	regular 3D morphology	2048:2068	regular 3D morphology	2048:2068	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	11	100	theme	good	2027:2030	arg1	cytocompatibility					2160:2176	good cytocompatibility	2155:2176	good cytocompatibility	2155:2176	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	2	101	theme	nanocrystals-chitosan-gelatin	440:468	arg1	scaffolds					497:505	alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds	411:505	alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes	411:743	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	5	102	dep	μm	1197:1198	arg1	to					1191:1192	to	1191:1192	to	1191:1192	With the increase in BCNs content, the pore size of Alg/BCNs-CS-GT composite scaffolds was gradually reduced from 200 μm to 70 μm.
34443588	8	103	theme	in	1467:1468	arg1	studies					1489:1495	the in vitro cytotoxicity studies	1463:1495	the in vitro cytotoxicity studies	1463:1495	Furthermore, the in vitro cytotoxicity studies indicated that the porous fiber network of BCNs could fully mimic the extracellular matrix structure, which promoted the adhesion and spreading of MG63 cells and MC3T3-E1 cells on the Alg/BCNs-CS-GT composite scaffolds.
34443588	6	104	from	embedded	1230:1237	arg1	matrix					1255:1260	the alginate matrix	1242:1260	the alginate matrix through the intermolecular hydrogen bond with alginate	1242:1315	Furthermore, BCNs were fully embedded in the alginate matrix through the intermolecular hydrogen bond with alginate.
34443588	2	105	theme	alginate/bacterial	411:428	arg1	scaffolds					497:505	alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds	411:505	alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes	411:743	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	8	106	from	spreading	1631:1639	arg1	scaffolds					1706:1714	the Alg/BCNs-CS-GT composite scaffolds	1677:1714	the Alg/BCNs-CS-GT composite scaffolds	1677:1714	Furthermore, the in vitro cytotoxicity studies indicated that the porous fiber network of BCNs could fully mimic the extracellular matrix structure, which promoted the adhesion and spreading of MG63 cells and MC3T3-E1 cells on the Alg/BCNs-CS-GT composite scaffolds.
34443588	11	107	theme	bone	2275:2278	arg1	field					2299:2303	the bone tissue engineering field	2271:2303	the bone tissue engineering field	2271:2303	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	8	108	theme	cells	1668:1672	arg1	adhesion					1618:1625	adhesion	1618:1625	adhesion	1618:1625	Furthermore, the in vitro cytotoxicity studies indicated that the porous fiber network of BCNs could fully mimic the extracellular matrix structure, which promoted the adhesion and spreading of MG63 cells and MC3T3-E1 cells on the Alg/BCNs-CS-GT composite scaffolds.
34443588	8	108	theme	cells	1668:1672	arg1	spreading					1631:1639	spreading	1631:1639	spreading	1631:1639	Furthermore, the in vitro cytotoxicity studies indicated that the porous fiber network of BCNs could fully mimic the extracellular matrix structure, which promoted the adhesion and spreading of MG63 cells and MC3T3-E1 cells on the Alg/BCNs-CS-GT composite scaffolds.
34443588	8	109	theme	cytotoxicity	1476:1487	arg1	studies					1489:1495	the in vitro cytotoxicity studies	1463:1495	the in vitro cytotoxicity studies	1463:1495	Furthermore, the in vitro cytotoxicity studies indicated that the porous fiber network of BCNs could fully mimic the extracellular matrix structure, which promoted the adhesion and spreading of MG63 cells and MC3T3-E1 cells on the Alg/BCNs-CS-GT composite scaffolds.
34443588	0	110	theme	Composite	89:97	arg1	Scaffolds					99:107	Alginate/Bacterial Cellulose Nanocrystals-Chitosan-Gelatin Composite Scaffolds	30:107	Alginate/Bacterial Cellulose Nanocrystals-Chitosan-Gelatin Composite Scaffolds	30:107	Fabrication and Evaluation of Alginate/Bacterial Cellulose Nanocrystals-Chitosan-Gelatin Composite Scaffolds.
34443588	5	111	theme	pore	1109:1112	arg1	size					1114:1117	the pore size	1105:1117	the pore size of Alg/BCNs-CS-GT composite scaffolds	1105:1155	With the increase in BCNs content, the pore size of Alg/BCNs-CS-GT composite scaffolds was gradually reduced from 200 μm to 70 μm.
34443588	2	112	theme	composite	487:495	arg1	scaffolds					497:505	alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds	411:505	alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes	411:743	To address these problems, we attempted to fabricate alginate/bacterial cellulose nanocrystals-chitosan-gelatin (Alg/BCNs-CS-GT) composite scaffolds using the combined method involving the incorporation of BCNs in the alginate matrix, internal gelation through the hydroxyapatite-d-glucono-δ-lactone (HAP-GDL) complex, and layer-by-layer (LBL) electrostatic assembly of polyelectrolytes.
34443588	8	113	theme	composite	1696:1704	arg1	scaffolds					1706:1714	the Alg/BCNs-CS-GT composite scaffolds	1677:1714	the Alg/BCNs-CS-GT composite scaffolds	1677:1714	Furthermore, the in vitro cytotoxicity studies indicated that the porous fiber network of BCNs could fully mimic the extracellular matrix structure, which promoted the adhesion and spreading of MG63 cells and MC3T3-E1 cells on the Alg/BCNs-CS-GT composite scaffolds.
34443588	10	114	theme	composite	1975:1983	arg1	scaffolds					1985:1993	the Alg/BCNs-CS-GT composite scaffolds	1956:1993	the Alg/BCNs-CS-GT composite scaffolds	1956:1993	Based on the effect of BCNs on the cytocompatibility of composite scaffolds, the optimum BCNs content for the Alg/BCNs-CS-GT composite scaffolds was 0.2% (w/v).
34443588	7	115	dep	swelling	1379:1386	arg1	the					1375:1377	the	1375:1377	the	1375:1377	Moreover, the addition of BCNs could effectively control the swelling and biodegradation of the Alg/BCNs-CS-GT composite scaffolds.
34443588	11	116	from	scaffold	2259:2266	arg1	field					2299:2303	the bone tissue engineering field	2271:2303	the bone tissue engineering field	2271:2303	On the basis of good merits, such as regular 3D morphology, well-developed pore structure, controlled swelling and biodegradation behavior, and good cytocompatibility, the Alg/BCNs-CS-GT composite scaffolds may exhibit great potential as the ideal scaffold in the bone tissue engineering field.
34443588	8	117	theme	fiber	1523:1527	arg1	network					1529:1535	the porous fiber network	1512:1535	the porous fiber network of BCNs	1512:1543	Furthermore, the in vitro cytotoxicity studies indicated that the porous fiber network of BCNs could fully mimic the extracellular matrix structure, which promoted the adhesion and spreading of MG63 cells and MC3T3-E1 cells on the Alg/BCNs-CS-GT composite scaffolds.
32815934	0	0	theme	cold-extruded	84:96	arg1	fettuccine					126:135	cold-extruded rice/white bean gluten-free fettuccine	84:135	cold-extruded rice/white bean gluten-free fettuccine	84:135	Cooking and sensorial quality, nutritional composition and functional properties of cold-extruded rice/white bean gluten-free fettuccine fortified with whole carob fruit flour.
32815934	4	1	theme	cooking	880:886	arg1	process					888:894	The cooking process	876:894	The cooking process	876:894	The cooking process increased (p < 0.05) the protein and total dietary fibre content but reduced the total and resistant starch.
32815934	4	2	theme	fibre	947:951	arg1	content					953:959	the protein and total dietary fibre content	917:959	content	953:959	The cooking process increased (p < 0.05) the protein and total dietary fibre content but reduced the total and resistant starch.
32815934	2	3	theme	sensorial	449:457	arg1	quality					459:465	the cooking and sensorial quality	433:465	the cooking and sensorial quality of uncooked and cooked pasta	433:494	The proximate composition, total and resistant starch, and total, soluble and insoluble dietary fibre content as well as the cooking and sensorial quality of uncooked and cooked pasta were determined.
32815934	3	4	theme	carob	644:648	arg1	fruit					650:654	the carob fruit	640:654	the carob fruit	640:654	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	2	5	theme	total	339:343	arg1	starch					359:364	total and resistant starch	339:364	total and resistant starch	339:364	The proximate composition, total and resistant starch, and total, soluble and insoluble dietary fibre content as well as the cooking and sensorial quality of uncooked and cooked pasta were determined.
32815934	4	6	theme	dietary	939:945	arg1	content					953:959	the protein and total dietary fibre content	917:959	content	953:959	The cooking process increased (p < 0.05) the protein and total dietary fibre content but reduced the total and resistant starch.
32815934	0	7	theme	bean	109:112	arg1	fettuccine					126:135	cold-extruded rice/white bean gluten-free fettuccine	84:135	cold-extruded rice/white bean gluten-free fettuccine	84:135	Cooking and sensorial quality, nutritional composition and functional properties of cold-extruded rice/white bean gluten-free fettuccine fortified with whole carob fruit flour.
32815934	4	8	dep	increased	896:904	arg1	<					909:909	p < 0.05	907:914	p < 0.05	907:914	The cooking process increased (p < 0.05) the protein and total dietary fibre content but reduced the total and resistant starch.
32815934	5	9	theme	total	1047:1051	arg1	content					1067:1073	the total dietary fibre content	1043:1073	the total dietary fibre content	1043:1073	The addition of carob fruit increased the total dietary fibre content, thus improving the functional value of fettuccine.
32815934	3	10	theme	average	799:805	arg1	3.6-fold					807:814	average 3.6-fold	799:814	average 3.6-fold	799:814	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	5	11	theme	fruit	1027:1031	arg1	addition					1009:1016	The addition	1005:1016	The addition of carob fruit	1005:1031	The addition of carob fruit increased the total dietary fibre content, thus improving the functional value of fettuccine.
32815934	2	12	theme	pasta	490:494	arg1	composition					326:336	The proximate composition	312:336	The proximate composition	312:336	The proximate composition, total and resistant starch, and total, soluble and insoluble dietary fibre content as well as the cooking and sensorial quality of uncooked and cooked pasta were determined.
32815934	2	12	theme	pasta	490:494	arg1	quality					459:465	the cooking and sensorial quality	433:465	the cooking and sensorial quality of uncooked and cooked pasta	433:494	The proximate composition, total and resistant starch, and total, soluble and insoluble dietary fibre content as well as the cooking and sensorial quality of uncooked and cooked pasta were determined.
32815934	2	12	theme	pasta	490:494	arg1	content					414:420	total, soluble and insoluble dietary fibre content	371:420	total, soluble and insoluble dietary fibre content	371:420	The proximate composition, total and resistant starch, and total, soluble and insoluble dietary fibre content as well as the cooking and sensorial quality of uncooked and cooked pasta were determined.
32815934	2	12	theme	pasta	490:494	arg1	starch					359:364	total and resistant starch	339:364	total and resistant starch	339:364	The proximate composition, total and resistant starch, and total, soluble and insoluble dietary fibre content as well as the cooking and sensorial quality of uncooked and cooked pasta were determined.
32815934	3	13	theme	healthy	683:689	arg1	profiles					691:698	better nutritional and healthy profiles	660:698	better nutritional and healthy profiles	660:698	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	6	14	theme	sensorial	1143:1151	arg1	analysis					1153:1160	the sensorial analysis	1139:1160	the sensorial analysis	1139:1160	Considering the sensorial analysis, fettuccine produced with 40% bean and 10% carob could be well accepted by consumers and can be advised as a functional food.
32815934	3	15	from	high	779:782	arg1	protein					787:793	protein	787:793	protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold)	787:873	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	0	16	theme	fettuccine	126:135	arg1	Cooking					0:6	Cooking	0:6	Cooking	0:6	Cooking and sensorial quality, nutritional composition and functional properties of cold-extruded rice/white bean gluten-free fettuccine fortified with whole carob fruit flour.
32815934	0	16	theme	fettuccine	126:135	arg1	quality					22:28	sensorial quality	12:28	sensorial quality	12:28	Cooking and sensorial quality, nutritional composition and functional properties of cold-extruded rice/white bean gluten-free fettuccine fortified with whole carob fruit flour.
32815934	0	16	theme	fettuccine	126:135	arg1	composition					43:53	nutritional composition	31:53	nutritional composition	31:53	Cooking and sensorial quality, nutritional composition and functional properties of cold-extruded rice/white bean gluten-free fettuccine fortified with whole carob fruit flour.
32815934	0	16	theme	fettuccine	126:135	arg1	properties					70:79	functional properties	59:79	functional properties	59:79	Cooking and sensorial quality, nutritional composition and functional properties of cold-extruded rice/white bean gluten-free fettuccine fortified with whole carob fruit flour.
32815934	6	17	theme	40	1188:1189	arg1	%					1190:1190	%	1190:1190	%	1190:1190	Considering the sensorial analysis, fettuccine produced with 40% bean and 10% carob could be well accepted by consumers and can be advised as a functional food.
32815934	1	18	theme	different	179:187	arg1	fettuccine					223:232	A different rice/white bean-based gluten-free fettuccine	177:232	A different rice/white bean-based gluten-free fettuccine (rice 0-100%, bean 0-100%) fortified with 10% carob fruit	177:290	A different rice/white bean-based gluten-free fettuccine (rice 0-100%, bean 0-100%) fortified with 10% carob fruit has been developed.
32815934	0	19	theme	gluten-free	114:124	arg1	fettuccine					126:135	cold-extruded rice/white bean gluten-free fettuccine	84:135	cold-extruded rice/white bean gluten-free fettuccine	84:135	Cooking and sensorial quality, nutritional composition and functional properties of cold-extruded rice/white bean gluten-free fettuccine fortified with whole carob fruit flour.
32815934	3	20	theme	nutritional	667:677	arg1	profiles					691:698	better nutritional and healthy profiles	660:698	better nutritional and healthy profiles	660:698	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	5	21	theme	functional	1095:1104	arg1	value					1106:1110	the functional value	1091:1110	the functional value of fettuccine	1091:1124	The addition of carob fruit increased the total dietary fibre content, thus improving the functional value of fettuccine.
32815934	1	22	theme	rice/white	189:198	arg1	fettuccine					223:232	A different rice/white bean-based gluten-free fettuccine	177:232	A different rice/white bean-based gluten-free fettuccine (rice 0-100%, bean 0-100%) fortified with 10% carob fruit	177:290	A different rice/white bean-based gluten-free fettuccine (rice 0-100%, bean 0-100%) fortified with 10% carob fruit has been developed.
32815934	3	23	from	low	754:756	arg1	fat					761:763	fat	761:763	fat	761:763	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	5	24	theme	dietary	1053:1059	arg1	content					1067:1073	the total dietary fibre content	1043:1073	the total dietary fibre content	1043:1073	The addition of carob fruit increased the total dietary fibre content, thus improving the functional value of fettuccine.
32815934	3	25	theme	commercial	709:718	arg1	pasta					737:741	the commercial gluten-free rice pasta	705:741	the commercial gluten-free rice pasta	705:741	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	26	theme	fettuccine	539:548	arg1	forms					550:554	All the novel gluten-free fettuccine forms	513:554	All the novel gluten-free fettuccine forms	513:554	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	27	theme	resistant	822:830	arg1	starch					832:837	resistant starch	822:837	resistant starch (16%)	822:843	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	27	theme	resistant	822:830	arg1	%					842:842	16%	840:842	16%	840:842	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	1	28	theme	bean-based	200:209	arg1	fettuccine					223:232	A different rice/white bean-based gluten-free fettuccine	177:232	A different rice/white bean-based gluten-free fettuccine (rice 0-100%, bean 0-100%) fortified with 10% carob fruit	177:290	A different rice/white bean-based gluten-free fettuccine (rice 0-100%, bean 0-100%) fortified with 10% carob fruit has been developed.
32815934	1	29	theme	10	276:277	arg1	%					278:278	%	278:278	%	278:278	A different rice/white bean-based gluten-free fettuccine (rice 0-100%, bean 0-100%) fortified with 10% carob fruit has been developed.
32815934	4	30	theme	p	907:907	arg1	<					909:909	p < 0.05	907:914	p < 0.05	907:914	The cooking process increased (p < 0.05) the protein and total dietary fibre content but reduced the total and resistant starch.
32815934	4	31	theme	total	977:981	arg1	starch					997:1002	the total and resistant starch	973:1002	the total and resistant starch	973:1002	The cooking process increased (p < 0.05) the protein and total dietary fibre content but reduced the total and resistant starch.
32815934	1	32	theme	gluten-free	211:221	arg1	fettuccine					223:232	A different rice/white bean-based gluten-free fettuccine	177:232	A different rice/white bean-based gluten-free fettuccine (rice 0-100%, bean 0-100%) fortified with 10% carob fruit	177:290	A different rice/white bean-based gluten-free fettuccine (rice 0-100%, bean 0-100%) fortified with 10% carob fruit has been developed.
32815934	6	33	theme	functional	1271:1280	arg1	food					1282:1285	a functional food	1269:1285	a functional food	1269:1285	Considering the sensorial analysis, fettuccine produced with 40% bean and 10% carob could be well accepted by consumers and can be advised as a functional food.
32815934	6	33	theme	functional	1271:1280	arg1	fettuccine					1163:1172	fettuccine	1163:1172	fettuccine produced with 40% bean and 10% carob	1163:1209	Considering the sensorial analysis, fettuccine produced with 40% bean and 10% carob could be well accepted by consumers and can be advised as a functional food.
32815934	1	34	theme	%	278:278	arg1	fruit					286:290	10% carob fruit	276:290	10% carob fruit	276:290	A different rice/white bean-based gluten-free fettuccine (rice 0-100%, bean 0-100%) fortified with 10% carob fruit has been developed.
32815934	6	35	theme	%	1203:1203	arg1	carob					1205:1209	10% carob	1201:1209	10% carob	1201:1209	Considering the sensorial analysis, fettuccine produced with 40% bean and 10% carob could be well accepted by consumers and can be advised as a functional food.
32815934	3	36	theme	novel	521:525	arg1	forms					550:554	All the novel gluten-free fettuccine forms	513:554	All the novel gluten-free fettuccine forms	513:554	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	2	37	theme	dietary	400:406	arg1	content					414:420	total, soluble and insoluble dietary fibre content	371:420	total, soluble and insoluble dietary fibre content	371:420	The proximate composition, total and resistant starch, and total, soluble and insoluble dietary fibre content as well as the cooking and sensorial quality of uncooked and cooked pasta were determined.
32815934	0	38	theme	sensorial	12:20	arg1	quality					22:28	sensorial quality	12:28	sensorial quality	12:28	Cooking and sensorial quality, nutritional composition and functional properties of cold-extruded rice/white bean gluten-free fettuccine fortified with whole carob fruit flour.
32815934	1	39	theme	carob	280:284	arg1	fruit					286:290	10% carob fruit	276:290	10% carob fruit	276:290	A different rice/white bean-based gluten-free fettuccine (rice 0-100%, bean 0-100%) fortified with 10% carob fruit has been developed.
32815934	6	40	theme	10	1201:1202	arg1	%					1203:1203	%	1203:1203	%	1203:1203	Considering the sensorial analysis, fettuccine produced with 40% bean and 10% carob could be well accepted by consumers and can be advised as a functional food.
32815934	3	41	contain	had	656:658	arg2	profiles					691:698	better nutritional and healthy profiles	660:698	better nutritional and healthy profiles	660:698	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	41	contain	had	656:658	arg1	those					623:627	those	623:627	those	623:627	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	4	42	theme	resistant	987:995	arg1	starch					997:1002	the total and resistant starch	973:1002	the total and resistant starch	973:1002	The cooking process increased (p < 0.05) the protein and total dietary fibre content but reduced the total and resistant starch.
32815934	2	43	theme	insoluble	390:398	arg1	content					414:420	total, soluble and insoluble dietary fibre content	371:420	total, soluble and insoluble dietary fibre content	371:420	The proximate composition, total and resistant starch, and total, soluble and insoluble dietary fibre content as well as the cooking and sensorial quality of uncooked and cooked pasta were determined.
32815934	0	44	theme	nutritional	31:41	arg1	composition					43:53	nutritional composition	31:53	nutritional composition	31:53	Cooking and sensorial quality, nutritional composition and functional properties of cold-extruded rice/white bean gluten-free fettuccine fortified with whole carob fruit flour.
32815934	5	45	theme	fibre	1061:1065	arg1	content					1067:1073	the total dietary fibre content	1043:1073	the total dietary fibre content	1043:1073	The addition of carob fruit increased the total dietary fibre content, thus improving the functional value of fettuccine.
32815934	0	46	theme	carob	158:162	arg1	flour					170:174	whole carob fruit flour	152:174	whole carob fruit flour	152:174	Cooking and sensorial quality, nutritional composition and functional properties of cold-extruded rice/white bean gluten-free fettuccine fortified with whole carob fruit flour.
32815934	3	47	dep	low	754:756	arg1	10-fold					766:772	10-fold	766:772	10-fold	766:772	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	48	theme	cooking	568:574	arg1	quality					576:582	good cooking quality	563:582	good cooking quality (cooking loss < 10%)	563:603	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	48	theme	cooking	568:574	arg1	%					602:602	cooking loss < 10%	585:602	cooking loss < 10%	585:602	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	49	contain	containing	629:638	arg1	those					623:627	those	623:627	those	623:627	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	49	contain	containing	629:638	arg2	fruit					650:654	the carob fruit	640:654	the carob fruit	640:654	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	1	50	theme	rice	235:238	arg1	%					245:245	rice 0-100%	235:245	rice 0-100%	235:245	A different rice/white bean-based gluten-free fettuccine (rice 0-100%, bean 0-100%) fortified with 10% carob fruit has been developed.
32815934	1	50	theme	rice	235:238	arg1	%					258:258	bean 0-100%	248:258	bean 0-100%	248:258	A different rice/white bean-based gluten-free fettuccine (rice 0-100%, bean 0-100%) fortified with 10% carob fruit has been developed.
32815934	2	51	theme	cooking	437:443	arg1	quality					459:465	the cooking and sensorial quality	433:465	the cooking and sensorial quality of uncooked and cooked pasta	433:494	The proximate composition, total and resistant starch, and total, soluble and insoluble dietary fibre content as well as the cooking and sensorial quality of uncooked and cooked pasta were determined.
32815934	0	52	theme	whole	152:156	arg1	flour					170:174	whole carob fruit flour	152:174	whole carob fruit flour	152:174	Cooking and sensorial quality, nutritional composition and functional properties of cold-extruded rice/white bean gluten-free fettuccine fortified with whole carob fruit flour.
32815934	2	53	theme	soluble	378:384	arg1	content					414:420	total, soluble and insoluble dietary fibre content	371:420	total, soluble and insoluble dietary fibre content	371:420	The proximate composition, total and resistant starch, and total, soluble and insoluble dietary fibre content as well as the cooking and sensorial quality of uncooked and cooked pasta were determined.
32815934	2	54	theme	cooked	483:488	arg1	pasta					490:494	uncooked and cooked pasta	470:494	uncooked and cooked pasta	470:494	The proximate composition, total and resistant starch, and total, soluble and insoluble dietary fibre content as well as the cooking and sensorial quality of uncooked and cooked pasta were determined.
32815934	5	55	theme	carob	1021:1025	arg1	fruit					1027:1031	carob fruit	1021:1031	carob fruit	1021:1031	The addition of carob fruit increased the total dietary fibre content, thus improving the functional value of fettuccine.
32815934	6	56	theme	%	1190:1190	arg1	bean					1192:1195	40% bean	1188:1195	40% bean	1188:1195	Considering the sensorial analysis, fettuccine produced with 40% bean and 10% carob could be well accepted by consumers and can be advised as a functional food.
32815934	3	57	theme	gluten-free	720:730	arg1	pasta					737:741	the commercial gluten-free rice pasta	705:741	the commercial gluten-free rice pasta	705:741	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	58	with	protein	787:793	arg1	starch					832:837	resistant starch	822:837	resistant starch (16%)	822:843	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	58	with	protein	787:793	arg1	%					842:842	16%	840:842	16%	840:842	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	58	with	protein	787:793	arg1	2.4-fold					865:872	2.4-fold	865:872	2.4-fold	865:872	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	58	with	protein	787:793	arg1	fibres					857:862	dietary fibres	849:862	dietary fibres (2.4-fold)	849:873	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	59	theme	rice	732:735	arg1	pasta					737:741	the commercial gluten-free rice pasta	705:741	the commercial gluten-free rice pasta	705:741	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	0	60	theme	fruit	164:168	arg1	flour					170:174	whole carob fruit flour	152:174	whole carob fruit flour	152:174	Cooking and sensorial quality, nutritional composition and functional properties of cold-extruded rice/white bean gluten-free fettuccine fortified with whole carob fruit flour.
32815934	3	61	theme	good	563:566	arg1	quality					576:582	good cooking quality	563:582	good cooking quality (cooking loss < 10%)	563:603	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	61	theme	good	563:566	arg1	%					602:602	cooking loss < 10%	585:602	cooking loss < 10%	585:602	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	62	theme	dietary	849:855	arg1	2.4-fold					865:872	2.4-fold	865:872	2.4-fold	865:872	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	62	theme	dietary	849:855	arg1	fibres					857:862	dietary fibres	849:862	dietary fibres (2.4-fold)	849:873	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	63	theme	cooking	585:591	arg1	quality					576:582	good cooking quality	563:582	good cooking quality (cooking loss < 10%)	563:603	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	63	theme	cooking	585:591	arg1	%					602:602	cooking loss < 10%	585:602	cooking loss < 10%	585:602	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	64	theme	loss	593:596	arg1	quality					576:582	good cooking quality	563:582	good cooking quality (cooking loss < 10%)	563:603	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	64	theme	loss	593:596	arg1	%					602:602	cooking loss < 10%	585:602	cooking loss < 10%	585:602	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	2	65	theme	proximate	316:324	arg1	composition					326:336	The proximate composition	312:336	The proximate composition	312:336	The proximate composition, total and resistant starch, and total, soluble and insoluble dietary fibre content as well as the cooking and sensorial quality of uncooked and cooked pasta were determined.
32815934	3	66	from	protein	787:793	arg1	high					779:782	high	779:782	high	779:782	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	66	from	protein	787:793	arg1	3.6-fold					807:814	average 3.6-fold	799:814	average 3.6-fold	799:814	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	67	from	fat	761:763	arg1	low					754:756	low	754:756	low	754:756	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	68	theme	<	598:598	arg1	quality					576:582	good cooking quality	563:582	good cooking quality (cooking loss < 10%)	563:603	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	68	theme	<	598:598	arg1	%					602:602	cooking loss < 10%	585:602	cooking loss < 10%	585:602	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	3	69	theme	gluten-free	527:537	arg1	forms					550:554	All the novel gluten-free fettuccine forms	513:554	All the novel gluten-free fettuccine forms	513:554	All the novel gluten-free fettuccine forms showed good cooking quality (cooking loss < 10%) highlighting that those containing the carob fruit had better nutritional and healthy profiles than the commercial gluten-free rice pasta; they were low in fat (10-fold) and high in protein (on average 3.6-fold) with resistant starch (16%) and dietary fibres (2.4-fold).
32815934	2	70	theme	uncooked	470:477	arg1	pasta					490:494	uncooked and cooked pasta	470:494	uncooked and cooked pasta	470:494	The proximate composition, total and resistant starch, and total, soluble and insoluble dietary fibre content as well as the cooking and sensorial quality of uncooked and cooked pasta were determined.
32815934	4	71	theme	total	933:937	arg1	content					953:959	the protein and total dietary fibre content	917:959	content	953:959	The cooking process increased (p < 0.05) the protein and total dietary fibre content but reduced the total and resistant starch.
32815934	1	72	dep	fettuccine	223:232	arg1	%					245:245	rice 0-100%	235:245	rice 0-100%	235:245	A different rice/white bean-based gluten-free fettuccine (rice 0-100%, bean 0-100%) fortified with 10% carob fruit has been developed.
32815934	1	72	dep	fettuccine	223:232	arg1	%					258:258	bean 0-100%	248:258	bean 0-100%	248:258	A different rice/white bean-based gluten-free fettuccine (rice 0-100%, bean 0-100%) fortified with 10% carob fruit has been developed.
32815934	2	73	theme	total	371:375	arg1	content					414:420	total, soluble and insoluble dietary fibre content	371:420	total, soluble and insoluble dietary fibre content	371:420	The proximate composition, total and resistant starch, and total, soluble and insoluble dietary fibre content as well as the cooking and sensorial quality of uncooked and cooked pasta were determined.
32815934	1	74	theme	bean	248:251	arg1	%					245:245	rice 0-100%	235:245	rice 0-100%	235:245	A different rice/white bean-based gluten-free fettuccine (rice 0-100%, bean 0-100%) fortified with 10% carob fruit has been developed.
32815934	1	74	theme	bean	248:251	arg1	%					258:258	bean 0-100%	248:258	bean 0-100%	248:258	A different rice/white bean-based gluten-free fettuccine (rice 0-100%, bean 0-100%) fortified with 10% carob fruit has been developed.
32815934	0	75	theme	functional	59:68	arg1	properties					70:79	functional properties	59:79	functional properties	59:79	Cooking and sensorial quality, nutritional composition and functional properties of cold-extruded rice/white bean gluten-free fettuccine fortified with whole carob fruit flour.
32815934	2	76	theme	fibre	408:412	arg1	content					414:420	total, soluble and insoluble dietary fibre content	371:420	total, soluble and insoluble dietary fibre content	371:420	The proximate composition, total and resistant starch, and total, soluble and insoluble dietary fibre content as well as the cooking and sensorial quality of uncooked and cooked pasta were determined.
32815934	2	77	theme	resistant	349:357	arg1	starch					359:364	total and resistant starch	339:364	total and resistant starch	339:364	The proximate composition, total and resistant starch, and total, soluble and insoluble dietary fibre content as well as the cooking and sensorial quality of uncooked and cooked pasta were determined.
32815934	5	78	theme	fettuccine	1115:1124	arg1	value					1106:1110	the functional value	1091:1110	the functional value of fettuccine	1091:1124	The addition of carob fruit increased the total dietary fibre content, thus improving the functional value of fettuccine.
32815934	0	79	theme	rice/white	98:107	arg1	fettuccine					126:135	cold-extruded rice/white bean gluten-free fettuccine	84:135	cold-extruded rice/white bean gluten-free fettuccine	84:135	Cooking and sensorial quality, nutritional composition and functional properties of cold-extruded rice/white bean gluten-free fettuccine fortified with whole carob fruit flour.
34507721	4	0	theme	more	869:872	arg1	Bacteroides					874:884	more Bacteroides	869:884	more Bacteroides	869:884	Further comparison of Samples A and C demonstrated that Sample C with the relatively strong SCSPsj utilization capability possessed more Parabacteroides while Sample A contained more Bacteroides.
34507721	8	1	theme	sulfated	1626:1633	arg1	polysaccharides					1648:1662	sulfated sea cucumber polysaccharides	1626:1662	sulfated sea cucumber polysaccharides	1626:1662	Thus, to obtain better functional outcome for sulfated sea cucumber polysaccharides and sea cucumber, more attention needs to be paid to the effects of inter-individual differences in microbiota community structure.
34507721	3	2	from	degrees	660:666	arg1	fermentation					677:688	vitro fermentation	671:688	vitro fermentation	671:688	Gut microbiota communities from 6 donors (A ~ F) utilized the polysaccharides to different degrees in vitro fermentation.
34507721	7	3	theme	Parabacteroides	1434:1448	arg1	distasonis					1450:1459	Parabacteroides distasonis	1434:1459	Parabacteroides distasonis	1434:1459	Our findings revealed that certain key members of gut microbiota, such as Parabacteroides distasonis, are critical for SCSPsj utilization in gut so as to influence the benefits of the polysaccharide supplement for host.
34507721	8	4	theme	cucumber	1639:1646	arg1	polysaccharides					1648:1662	sulfated sea cucumber polysaccharides	1626:1662	sulfated sea cucumber polysaccharides	1626:1662	Thus, to obtain better functional outcome for sulfated sea cucumber polysaccharides and sea cucumber, more attention needs to be paid to the effects of inter-individual differences in microbiota community structure.
34507721	6	5	theme	beneficial	1238:1247	arg1	metabolites					1249:1259	more beneficial metabolites	1233:1259	more beneficial metabolites	1233:1259	Moreover, short chain fatty acids and the metabolite profiles of Samples A and C were also compared, and the results showed that more beneficial metabolites were accumulated by the microbiota community consuming more sulfated sea cucumber polysaccharides.
34507721	7	6	theme	microbiota	1414:1423	arg1	distasonis					1450:1459	Parabacteroides distasonis	1434:1459	Parabacteroides distasonis	1434:1459	Our findings revealed that certain key members of gut microbiota, such as Parabacteroides distasonis, are critical for SCSPsj utilization in gut so as to influence the benefits of the polysaccharide supplement for host.
34507721	7	6	theme	microbiota	1414:1423	arg1	members					1399:1405	certain key members	1387:1405	certain key members	1387:1405	Our findings revealed that certain key members of gut microbiota, such as Parabacteroides distasonis, are critical for SCSPsj utilization in gut so as to influence the benefits of the polysaccharide supplement for host.
34507721	1	7	theme	high-valued	182:192	arg1	cucumber					132:139	Sea cucumber	128:139	Sea cucumber Stichopus japonicus	128:159	Sea cucumber Stichopus japonicus has been consumed as high-valued seafood in Asian, and its sulfated polysaccharide (SCSPsj) has been inferred to benefit the host health via modulating gut microbiota composition.
34507721	1	7	theme	high-valued	182:192	arg1	seafood					194:200	high-valued seafood	182:200	high-valued seafood in Asian	182:209	Sea cucumber Stichopus japonicus has been consumed as high-valued seafood in Asian, and its sulfated polysaccharide (SCSPsj) has been inferred to benefit the host health via modulating gut microbiota composition.
34507721	6	8	theme	metabolite	1146:1155	arg1	profiles					1157:1164	the metabolite profiles	1142:1164	the metabolite profiles of Samples A and C	1142:1183	Moreover, short chain fatty acids and the metabolite profiles of Samples A and C were also compared, and the results showed that more beneficial metabolites were accumulated by the microbiota community consuming more sulfated sea cucumber polysaccharides.
34507721	6	8	theme	metabolite	1146:1155	arg1	A					1177:1177	A	1177:1177	A	1177:1177	Moreover, short chain fatty acids and the metabolite profiles of Samples A and C were also compared, and the results showed that more beneficial metabolites were accumulated by the microbiota community consuming more sulfated sea cucumber polysaccharides.
34507721	6	8	theme	metabolite	1146:1155	arg1	C					1183:1183	C	1183:1183	C	1183:1183	Moreover, short chain fatty acids and the metabolite profiles of Samples A and C were also compared, and the results showed that more beneficial metabolites were accumulated by the microbiota community consuming more sulfated sea cucumber polysaccharides.
34507721	6	9	theme	cucumber	1334:1341	arg1	polysaccharides					1343:1357	more sulfated sea cucumber polysaccharides	1316:1357	more sulfated sea cucumber polysaccharides	1316:1357	Moreover, short chain fatty acids and the metabolite profiles of Samples A and C were also compared, and the results showed that more beneficial metabolites were accumulated by the microbiota community consuming more sulfated sea cucumber polysaccharides.
34507721	8	10	theme	inter-individual	1732:1747	arg1	differences					1749:1759	inter-individual differences	1732:1759	inter-individual differences in microbiota community structure	1732:1793	Thus, to obtain better functional outcome for sulfated sea cucumber polysaccharides and sea cucumber, more attention needs to be paid to the effects of inter-individual differences in microbiota community structure.
34507721	7	11	theme	key	1395:1397	arg1	distasonis					1450:1459	Parabacteroides distasonis	1434:1459	Parabacteroides distasonis	1434:1459	Our findings revealed that certain key members of gut microbiota, such as Parabacteroides distasonis, are critical for SCSPsj utilization in gut so as to influence the benefits of the polysaccharide supplement for host.
34507721	7	11	theme	key	1395:1397	arg1	members					1399:1405	certain key members	1387:1405	certain key members	1387:1405	Our findings revealed that certain key members of gut microbiota, such as Parabacteroides distasonis, are critical for SCSPsj utilization in gut so as to influence the benefits of the polysaccharide supplement for host.
34507721	6	12	theme	sulfated	1321:1328	arg1	polysaccharides					1343:1357	more sulfated sea cucumber polysaccharides	1316:1357	more sulfated sea cucumber polysaccharides	1316:1357	Moreover, short chain fatty acids and the metabolite profiles of Samples A and C were also compared, and the results showed that more beneficial metabolites were accumulated by the microbiota community consuming more sulfated sea cucumber polysaccharides.
34507721	5	13	theme	critical	1047:1054	arg1	role					1056:1059	a critical role	1045:1059	a critical role	1045:1059	Further in vitro fermentation of SCSPsj with 10 Parabacteroides and Bacteroides species suggests that Parabacteroides distasonis, enriched in Sample C, plays a critical role in the utilization of the polysaccharides.
34507721	3	14	theme	Gut	569:571	arg1	communities					584:594	Gut microbiota communities	569:594	Gut microbiota communities from 6 donors (A ~ F)	569:616	Gut microbiota communities from 6 donors (A ~ F) utilized the polysaccharides to different degrees in vitro fermentation.
34507721	1	15	from	seafood	194:200	arg1	Asian					205:209	Asian	205:209	Asian	205:209	Sea cucumber Stichopus japonicus has been consumed as high-valued seafood in Asian, and its sulfated polysaccharide (SCSPsj) has been inferred to benefit the host health via modulating gut microbiota composition.
34507721	5	16	theme	SCSPsj	920:925	arg1	fermentation					904:915	Further in vitro fermentation	887:915	Further in vitro fermentation of SCSPsj with 10 Parabacteroides and Bacteroides species	887:973	Further in vitro fermentation of SCSPsj with 10 Parabacteroides and Bacteroides species suggests that Parabacteroides distasonis, enriched in Sample C, plays a critical role in the utilization of the polysaccharides.
34507721	4	17	theme	Samples	713:719	arg1	comparison					699:708	Further comparison	691:708	Further comparison of Samples A and C	691:727	Further comparison of Samples A and C demonstrated that Sample C with the relatively strong SCSPsj utilization capability possessed more Parabacteroides while Sample A contained more Bacteroides.
34507721	6	18	theme	microbiota	1285:1294	arg1	community					1296:1304	the microbiota community	1281:1304	the microbiota community consuming more sulfated sea cucumber polysaccharides	1281:1357	Moreover, short chain fatty acids and the metabolite profiles of Samples A and C were also compared, and the results showed that more beneficial metabolites were accumulated by the microbiota community consuming more sulfated sea cucumber polysaccharides.
34507721	1	19	theme	Sea	128:130	arg1	seafood					194:200	high-valued seafood	182:200	high-valued seafood in Asian	182:209	Sea cucumber Stichopus japonicus has been consumed as high-valued seafood in Asian, and its sulfated polysaccharide (SCSPsj) has been inferred to benefit the host health via modulating gut microbiota composition.
34507721	1	19	theme	Sea	128:130	arg1	cucumber					132:139	Sea cucumber	128:139	Sea cucumber Stichopus japonicus	128:159	Sea cucumber Stichopus japonicus has been consumed as high-valued seafood in Asian, and its sulfated polysaccharide (SCSPsj) has been inferred to benefit the host health via modulating gut microbiota composition.
34507721	5	20	theme	in	895:896	arg1	fermentation					904:915	Further in vitro fermentation	887:915	Further in vitro fermentation of SCSPsj with 10 Parabacteroides and Bacteroides species	887:973	Further in vitro fermentation of SCSPsj with 10 Parabacteroides and Bacteroides species suggests that Parabacteroides distasonis, enriched in Sample C, plays a critical role in the utilization of the polysaccharides.
34507721	0	21	from	response	15:22	arg1	manner					83:88	a differential manner	68:88	a differential manner	68:88	Gut microbiota response to sulfated sea cucumber polysaccharides in a differential manner using an in vitro fermentation model.
34507721	4	22	contain	possessed	813:821	arg2	Parabacteroides					828:842	more Parabacteroides	823:842	more Parabacteroides	823:842	Further comparison of Samples A and C demonstrated that Sample C with the relatively strong SCSPsj utilization capability possessed more Parabacteroides while Sample A contained more Bacteroides.
34507721	4	22	contain	possessed	813:821	arg1	C					754:754	Sample C	747:754	Sample C with the relatively strong SCSPsj utilization capability	747:811	Further comparison of Samples A and C demonstrated that Sample C with the relatively strong SCSPsj utilization capability possessed more Parabacteroides while Sample A contained more Bacteroides.
34507721	0	23	theme	differential	70:81	arg1	manner					83:88	a differential manner	68:88	a differential manner	68:88	Gut microbiota response to sulfated sea cucumber polysaccharides in a differential manner using an in vitro fermentation model.
34507721	1	24	theme	microbiota	317:326	arg1	composition					328:338	gut microbiota composition	313:338	gut microbiota composition	313:338	Sea cucumber Stichopus japonicus has been consumed as high-valued seafood in Asian, and its sulfated polysaccharide (SCSPsj) has been inferred to benefit the host health via modulating gut microbiota composition.
34507721	4	25	contain	contained	859:867	arg1	A					857:857	Sample A	850:857	Sample A	850:857	Further comparison of Samples A and C demonstrated that Sample C with the relatively strong SCSPsj utilization capability possessed more Parabacteroides while Sample A contained more Bacteroides.
34507721	4	25	contain	contained	859:867	arg2	Bacteroides					874:884	more Bacteroides	869:884	more Bacteroides	869:884	Further comparison of Samples A and C demonstrated that Sample C with the relatively strong SCSPsj utilization capability possessed more Parabacteroides while Sample A contained more Bacteroides.
34507721	2	26	theme	sequencing	499:508	arg1	analysis					510:517	16S rRNA gene sequencing analysis	485:517	16S rRNA gene sequencing analysis	485:517	The present study compared the responses of gut microbiota communities from different donors to SCSPsj, and the key bacteria were identified by 16S rRNA gene sequencing analysis and in vitro fermentation with specific bacteria.
34507721	4	27	theme	Sample	747:752	arg1	C					754:754	Sample C	747:754	Sample C with the relatively strong SCSPsj utilization capability	747:811	Further comparison of Samples A and C demonstrated that Sample C with the relatively strong SCSPsj utilization capability possessed more Parabacteroides while Sample A contained more Bacteroides.
34507721	0	28	dep	in	99:100	arg1	vitro					102:106	vitro	102:106	vitro	102:106	Gut microbiota response to sulfated sea cucumber polysaccharides in a differential manner using an in vitro fermentation model.
34507721	7	29	theme	supplement	1559:1568	arg1	benefits					1528:1535	the benefits	1524:1535	the benefits of the polysaccharide supplement for host	1524:1577	Our findings revealed that certain key members of gut microbiota, such as Parabacteroides distasonis, are critical for SCSPsj utilization in gut so as to influence the benefits of the polysaccharide supplement for host.
34507721	2	30	theme	rRNA	489:492	arg1	sequencing					499:508	16S rRNA gene sequencing	485:508	16S rRNA gene sequencing analysis	485:517	The present study compared the responses of gut microbiota communities from different donors to SCSPsj, and the key bacteria were identified by 16S rRNA gene sequencing analysis and in vitro fermentation with specific bacteria.
34507721	0	31	theme	in	99:100	arg1	model					121:125	an in vitro fermentation model	96:125	an in vitro fermentation model	96:125	Gut microbiota response to sulfated sea cucumber polysaccharides in a differential manner using an in vitro fermentation model.
34507721	0	32	theme	Gut	0:2	arg1	response					15:22	Gut microbiota response	0:22	Gut microbiota response to sulfated sea cucumber polysaccharides in a differential manner	0:88	Gut microbiota response to sulfated sea cucumber polysaccharides in a differential manner using an in vitro fermentation model.
34507721	2	33	theme	key	453:455	arg1	bacteria					457:464	the key bacteria	449:464	the key bacteria	449:464	The present study compared the responses of gut microbiota communities from different donors to SCSPsj, and the key bacteria were identified by 16S rRNA gene sequencing analysis and in vitro fermentation with specific bacteria.
34507721	3	34	used	utilized	618:625	arg2	communities					584:594	Gut microbiota communities	569:594	Gut microbiota communities from 6 donors (A ~ F)	569:616	Gut microbiota communities from 6 donors (A ~ F) utilized the polysaccharides to different degrees in vitro fermentation.
34507721	2	35	theme	microbiota	389:398	arg1	communities					400:410	gut microbiota communities	385:410	gut microbiota communities from different donors	385:432	The present study compared the responses of gut microbiota communities from different donors to SCSPsj, and the key bacteria were identified by 16S rRNA gene sequencing analysis and in vitro fermentation with specific bacteria.
34507721	0	36	theme	sulfated	27:34	arg1	polysaccharides					49:63	sulfated sea cucumber polysaccharides	27:63	sulfated sea cucumber polysaccharides	27:63	Gut microbiota response to sulfated sea cucumber polysaccharides in a differential manner using an in vitro fermentation model.
34507721	4	37	theme	more	823:826	arg1	Parabacteroides					828:842	more Parabacteroides	823:842	more Parabacteroides	823:842	Further comparison of Samples A and C demonstrated that Sample C with the relatively strong SCSPsj utilization capability possessed more Parabacteroides while Sample A contained more Bacteroides.
34507721	0	38	theme	cucumber	40:47	arg1	polysaccharides					49:63	sulfated sea cucumber polysaccharides	27:63	sulfated sea cucumber polysaccharides	27:63	Gut microbiota response to sulfated sea cucumber polysaccharides in a differential manner using an in vitro fermentation model.
34507721	2	39	with	analysis	510:517	arg1	bacteria					559:566	specific bacteria	550:566	specific bacteria	550:566	The present study compared the responses of gut microbiota communities from different donors to SCSPsj, and the key bacteria were identified by 16S rRNA gene sequencing analysis and in vitro fermentation with specific bacteria.
34507721	6	40	theme	chain	1120:1124	arg1	acids					1132:1136	short chain fatty acids	1114:1136	short chain fatty acids	1114:1136	Moreover, short chain fatty acids and the metabolite profiles of Samples A and C were also compared, and the results showed that more beneficial metabolites were accumulated by the microbiota community consuming more sulfated sea cucumber polysaccharides.
34507721	4	41	theme	SCSPsj	783:788	arg1	capability					802:811	the relatively strong SCSPsj utilization capability	761:811	the relatively strong SCSPsj utilization capability	761:811	Further comparison of Samples A and C demonstrated that Sample C with the relatively strong SCSPsj utilization capability possessed more Parabacteroides while Sample A contained more Bacteroides.
34507721	8	42	theme	sea	1668:1670	arg1	cucumber					1672:1679	sea cucumber	1668:1679	sea cucumber	1668:1679	Thus, to obtain better functional outcome for sulfated sea cucumber polysaccharides and sea cucumber, more attention needs to be paid to the effects of inter-individual differences in microbiota community structure.
34507721	1	43	theme	sulfated	220:227	arg1	SCSPsj					245:250	SCSPsj	245:250	SCSPsj	245:250	Sea cucumber Stichopus japonicus has been consumed as high-valued seafood in Asian, and its sulfated polysaccharide (SCSPsj) has been inferred to benefit the host health via modulating gut microbiota composition.
34507721	1	43	theme	sulfated	220:227	arg1	polysaccharide					229:242	its sulfated polysaccharide	216:242	its sulfated polysaccharide (SCSPsj)	216:251	Sea cucumber Stichopus japonicus has been consumed as high-valued seafood in Asian, and its sulfated polysaccharide (SCSPsj) has been inferred to benefit the host health via modulating gut microbiota composition.
34507721	5	44	theme	Sample	1029:1034	arg1	C					1036:1036	Sample C	1029:1036	Sample C	1029:1036	Further in vitro fermentation of SCSPsj with 10 Parabacteroides and Bacteroides species suggests that Parabacteroides distasonis, enriched in Sample C, plays a critical role in the utilization of the polysaccharides.
34507721	8	45	theme	functional	1603:1612	arg1	outcome					1614:1620	better functional outcome	1596:1620	better functional outcome	1596:1620	Thus, to obtain better functional outcome for sulfated sea cucumber polysaccharides and sea cucumber, more attention needs to be paid to the effects of inter-individual differences in microbiota community structure.
34507721	2	46	with	fermentation	532:543	arg1	bacteria					559:566	specific bacteria	550:566	specific bacteria	550:566	The present study compared the responses of gut microbiota communities from different donors to SCSPsj, and the key bacteria were identified by 16S rRNA gene sequencing analysis and in vitro fermentation with specific bacteria.
34507721	7	47	theme	SCSPsj	1479:1484	arg1	utilization					1486:1496	SCSPsj utilization	1479:1496	SCSPsj utilization in gut so as to influence the benefits of the polysaccharide supplement for host	1479:1577	Our findings revealed that certain key members of gut microbiota, such as Parabacteroides distasonis, are critical for SCSPsj utilization in gut so as to influence the benefits of the polysaccharide supplement for host.
34507721	3	48	theme	vitro	671:675	arg1	fermentation					677:688	vitro fermentation	671:688	vitro fermentation	671:688	Gut microbiota communities from 6 donors (A ~ F) utilized the polysaccharides to different degrees in vitro fermentation.
34507721	8	49	theme	community	1775:1783	arg1	structure					1785:1793	microbiota community structure	1764:1793	microbiota community structure	1764:1793	Thus, to obtain better functional outcome for sulfated sea cucumber polysaccharides and sea cucumber, more attention needs to be paid to the effects of inter-individual differences in microbiota community structure.
34507721	8	50	theme	sea	1635:1637	arg1	polysaccharides					1648:1662	sulfated sea cucumber polysaccharides	1626:1662	sulfated sea cucumber polysaccharides	1626:1662	Thus, to obtain better functional outcome for sulfated sea cucumber polysaccharides and sea cucumber, more attention needs to be paid to the effects of inter-individual differences in microbiota community structure.
34507721	2	51	dep	in	523:524	arg1	vitro					526:530	vitro	526:530	vitro	526:530	The present study compared the responses of gut microbiota communities from different donors to SCSPsj, and the key bacteria were identified by 16S rRNA gene sequencing analysis and in vitro fermentation with specific bacteria.
34507721	6	52	theme	Samples	1169:1175	arg1	profiles					1157:1164	the metabolite profiles	1142:1164	the metabolite profiles of Samples A and C	1142:1183	Moreover, short chain fatty acids and the metabolite profiles of Samples A and C were also compared, and the results showed that more beneficial metabolites were accumulated by the microbiota community consuming more sulfated sea cucumber polysaccharides.
34507721	6	52	theme	Samples	1169:1175	arg1	A					1177:1177	A	1177:1177	A	1177:1177	Moreover, short chain fatty acids and the metabolite profiles of Samples A and C were also compared, and the results showed that more beneficial metabolites were accumulated by the microbiota community consuming more sulfated sea cucumber polysaccharides.
34507721	6	52	theme	Samples	1169:1175	arg1	acids					1132:1136	short chain fatty acids	1114:1136	short chain fatty acids	1114:1136	Moreover, short chain fatty acids and the metabolite profiles of Samples A and C were also compared, and the results showed that more beneficial metabolites were accumulated by the microbiota community consuming more sulfated sea cucumber polysaccharides.
34507721	6	52	theme	Samples	1169:1175	arg1	C					1183:1183	C	1183:1183	C	1183:1183	Moreover, short chain fatty acids and the metabolite profiles of Samples A and C were also compared, and the results showed that more beneficial metabolites were accumulated by the microbiota community consuming more sulfated sea cucumber polysaccharides.
34507721	4	53	theme	Sample	850:855	arg1	A					857:857	Sample A	850:857	Sample A	850:857	Further comparison of Samples A and C demonstrated that Sample C with the relatively strong SCSPsj utilization capability possessed more Parabacteroides while Sample A contained more Bacteroides.
34507721	6	54	dep	profiles	1157:1164	arg1	profiles					1157:1164	the metabolite profiles	1142:1164	the metabolite profiles of Samples A and C	1142:1183	Moreover, short chain fatty acids and the metabolite profiles of Samples A and C were also compared, and the results showed that more beneficial metabolites were accumulated by the microbiota community consuming more sulfated sea cucumber polysaccharides.
34507721	6	54	dep	profiles	1157:1164	arg1	A					1177:1177	A	1177:1177	A	1177:1177	Moreover, short chain fatty acids and the metabolite profiles of Samples A and C were also compared, and the results showed that more beneficial metabolites were accumulated by the microbiota community consuming more sulfated sea cucumber polysaccharides.
34507721	6	54	dep	profiles	1157:1164	arg1	C					1183:1183	C	1183:1183	C	1183:1183	Moreover, short chain fatty acids and the metabolite profiles of Samples A and C were also compared, and the results showed that more beneficial metabolites were accumulated by the microbiota community consuming more sulfated sea cucumber polysaccharides.
34507721	4	55	with	C	754:754	arg1	capability					802:811	the relatively strong SCSPsj utilization capability	761:811	the relatively strong SCSPsj utilization capability	761:811	Further comparison of Samples A and C demonstrated that Sample C with the relatively strong SCSPsj utilization capability possessed more Parabacteroides while Sample A contained more Bacteroides.
34507721	2	56	theme	in	523:524	arg1	fermentation					532:543	in vitro fermentation	523:543	in vitro fermentation with specific bacteria	523:566	The present study compared the responses of gut microbiota communities from different donors to SCSPsj, and the key bacteria were identified by 16S rRNA gene sequencing analysis and in vitro fermentation with specific bacteria.
34507721	7	57	theme	gut	1410:1412	arg1	microbiota					1414:1423	gut microbiota	1410:1423	gut microbiota	1410:1423	Our findings revealed that certain key members of gut microbiota, such as Parabacteroides distasonis, are critical for SCSPsj utilization in gut so as to influence the benefits of the polysaccharide supplement for host.
34507721	6	58	theme	sea	1330:1332	arg1	polysaccharides					1343:1357	more sulfated sea cucumber polysaccharides	1316:1357	more sulfated sea cucumber polysaccharides	1316:1357	Moreover, short chain fatty acids and the metabolite profiles of Samples A and C were also compared, and the results showed that more beneficial metabolites were accumulated by the microbiota community consuming more sulfated sea cucumber polysaccharides.
34507721	3	59	theme	microbiota	573:582	arg1	communities					584:594	Gut microbiota communities	569:594	Gut microbiota communities from 6 donors (A ~ F)	569:616	Gut microbiota communities from 6 donors (A ~ F) utilized the polysaccharides to different degrees in vitro fermentation.
34507721	8	60	from	differences	1749:1759	arg1	structure					1785:1793	microbiota community structure	1764:1793	microbiota community structure	1764:1793	Thus, to obtain better functional outcome for sulfated sea cucumber polysaccharides and sea cucumber, more attention needs to be paid to the effects of inter-individual differences in microbiota community structure.
34507721	7	61	from	utilization	1486:1496	arg1	gut					1501:1503	gut	1501:1503	gut so as to influence the benefits of the polysaccharide supplement for host	1501:1577	Our findings revealed that certain key members of gut microbiota, such as Parabacteroides distasonis, are critical for SCSPsj utilization in gut so as to influence the benefits of the polysaccharide supplement for host.
34507721	8	62	from	structure	1785:1793	arg1	effects					1721:1727	the effects	1717:1727	the effects of inter-individual differences in microbiota community structure	1717:1793	Thus, to obtain better functional outcome for sulfated sea cucumber polysaccharides and sea cucumber, more attention needs to be paid to the effects of inter-individual differences in microbiota community structure.
34507721	6	63	theme	more	1316:1319	arg1	polysaccharides					1343:1357	more sulfated sea cucumber polysaccharides	1316:1357	more sulfated sea cucumber polysaccharides	1316:1357	Moreover, short chain fatty acids and the metabolite profiles of Samples A and C were also compared, and the results showed that more beneficial metabolites were accumulated by the microbiota community consuming more sulfated sea cucumber polysaccharides.
34507721	5	64	theme	polysaccharides	1087:1101	arg1	utilization					1068:1078	the utilization	1064:1078	the utilization of the polysaccharides	1064:1101	Further in vitro fermentation of SCSPsj with 10 Parabacteroides and Bacteroides species suggests that Parabacteroides distasonis, enriched in Sample C, plays a critical role in the utilization of the polysaccharides.
34507721	8	65	theme	differences	1749:1759	arg1	effects					1721:1727	the effects	1717:1727	the effects of inter-individual differences in microbiota community structure	1717:1793	Thus, to obtain better functional outcome for sulfated sea cucumber polysaccharides and sea cucumber, more attention needs to be paid to the effects of inter-individual differences in microbiota community structure.
34507721	2	66	theme	specific	550:557	arg1	bacteria					559:566	specific bacteria	550:566	specific bacteria	550:566	The present study compared the responses of gut microbiota communities from different donors to SCSPsj, and the key bacteria were identified by 16S rRNA gene sequencing analysis and in vitro fermentation with specific bacteria.
34507721	7	67	theme	certain	1387:1393	arg1	distasonis					1450:1459	Parabacteroides distasonis	1434:1459	Parabacteroides distasonis	1434:1459	Our findings revealed that certain key members of gut microbiota, such as Parabacteroides distasonis, are critical for SCSPsj utilization in gut so as to influence the benefits of the polysaccharide supplement for host.
34507721	7	67	theme	certain	1387:1393	arg1	members					1399:1405	certain key members	1387:1405	certain key members	1387:1405	Our findings revealed that certain key members of gut microbiota, such as Parabacteroides distasonis, are critical for SCSPsj utilization in gut so as to influence the benefits of the polysaccharide supplement for host.
34507721	4	68	theme	Further	691:697	arg1	comparison					699:708	Further comparison	691:708	Further comparison of Samples A and C	691:727	Further comparison of Samples A and C demonstrated that Sample C with the relatively strong SCSPsj utilization capability possessed more Parabacteroides while Sample A contained more Bacteroides.
34507721	8	69	theme	microbiota	1764:1773	arg1	structure					1785:1793	microbiota community structure	1764:1793	microbiota community structure	1764:1793	Thus, to obtain better functional outcome for sulfated sea cucumber polysaccharides and sea cucumber, more attention needs to be paid to the effects of inter-individual differences in microbiota community structure.
34507721	5	70	theme	Further	887:893	arg1	fermentation					904:915	Further in vitro fermentation	887:915	Further in vitro fermentation of SCSPsj with 10 Parabacteroides and Bacteroides species	887:973	Further in vitro fermentation of SCSPsj with 10 Parabacteroides and Bacteroides species suggests that Parabacteroides distasonis, enriched in Sample C, plays a critical role in the utilization of the polysaccharides.
34507721	2	71	from	donors	427:432	arg1	responses					372:380	the responses	368:380	the responses of gut microbiota communities from different donors to SCSPsj	368:442	The present study compared the responses of gut microbiota communities from different donors to SCSPsj, and the key bacteria were identified by 16S rRNA gene sequencing analysis and in vitro fermentation with specific bacteria.
34507721	2	71	from	donors	427:432	arg1	communities					400:410	gut microbiota communities	385:410	gut microbiota communities from different donors	385:432	The present study compared the responses of gut microbiota communities from different donors to SCSPsj, and the key bacteria were identified by 16S rRNA gene sequencing analysis and in vitro fermentation with specific bacteria.
34507721	1	72	theme	gut	313:315	arg1	composition					328:338	gut microbiota composition	313:338	gut microbiota composition	313:338	Sea cucumber Stichopus japonicus has been consumed as high-valued seafood in Asian, and its sulfated polysaccharide (SCSPsj) has been inferred to benefit the host health via modulating gut microbiota composition.
34507721	0	73	theme	fermentation	108:119	arg1	model					121:125	an in vitro fermentation model	96:125	an in vitro fermentation model	96:125	Gut microbiota response to sulfated sea cucumber polysaccharides in a differential manner using an in vitro fermentation model.
34507721	5	74	with	fermentation	904:915	arg1	Parabacteroides					935:949	10 Parabacteroides	932:949	10 Parabacteroides	932:949	Further in vitro fermentation of SCSPsj with 10 Parabacteroides and Bacteroides species suggests that Parabacteroides distasonis, enriched in Sample C, plays a critical role in the utilization of the polysaccharides.
34507721	5	74	with	fermentation	904:915	arg1	species					967:973	Bacteroides species	955:973	Bacteroides species	955:973	Further in vitro fermentation of SCSPsj with 10 Parabacteroides and Bacteroides species suggests that Parabacteroides distasonis, enriched in Sample C, plays a critical role in the utilization of the polysaccharides.
34507721	7	75	theme	polysaccharide	1544:1557	arg1	supplement					1559:1568	the polysaccharide supplement	1540:1568	the polysaccharide supplement	1540:1568	Our findings revealed that certain key members of gut microbiota, such as Parabacteroides distasonis, are critical for SCSPsj utilization in gut so as to influence the benefits of the polysaccharide supplement for host.
34507721	2	76	theme	gene	494:497	arg1	sequencing					499:508	16S rRNA gene sequencing	485:508	16S rRNA gene sequencing analysis	485:517	The present study compared the responses of gut microbiota communities from different donors to SCSPsj, and the key bacteria were identified by 16S rRNA gene sequencing analysis and in vitro fermentation with specific bacteria.
34507721	5	77	theme	Parabacteroides	989:1003	arg1	distasonis					1005:1014	Parabacteroides distasonis	989:1014	Parabacteroides distasonis	989:1014	Further in vitro fermentation of SCSPsj with 10 Parabacteroides and Bacteroides species suggests that Parabacteroides distasonis, enriched in Sample C, plays a critical role in the utilization of the polysaccharides.
34507721	2	78	theme	16S	485:487	arg1	sequencing					499:508	16S rRNA gene sequencing	485:508	16S rRNA gene sequencing analysis	485:517	The present study compared the responses of gut microbiota communities from different donors to SCSPsj, and the key bacteria were identified by 16S rRNA gene sequencing analysis and in vitro fermentation with specific bacteria.
34507721	3	79	from	donors	603:608	arg1	communities					584:594	Gut microbiota communities	569:594	Gut microbiota communities from 6 donors (A ~ F)	569:616	Gut microbiota communities from 6 donors (A ~ F) utilized the polysaccharides to different degrees in vitro fermentation.
34507721	0	80	theme	microbiota	4:13	arg1	response					15:22	Gut microbiota response	0:22	Gut microbiota response to sulfated sea cucumber polysaccharides in a differential manner	0:88	Gut microbiota response to sulfated sea cucumber polysaccharides in a differential manner using an in vitro fermentation model.
34507721	4	81	dep	Samples	713:719	arg1	C					727:727	C	727:727	C	727:727	Further comparison of Samples A and C demonstrated that Sample C with the relatively strong SCSPsj utilization capability possessed more Parabacteroides while Sample A contained more Bacteroides.
34507721	4	81	dep	Samples	713:719	arg1	A					721:721	A	721:721	A	721:721	Further comparison of Samples A and C demonstrated that Sample C with the relatively strong SCSPsj utilization capability possessed more Parabacteroides while Sample A contained more Bacteroides.
34507721	4	81	dep	Samples	713:719	arg1	Samples					713:719	Samples A and C	713:727	Samples A and C	713:727	Further comparison of Samples A and C demonstrated that Sample C with the relatively strong SCSPsj utilization capability possessed more Parabacteroides while Sample A contained more Bacteroides.
34507721	8	82	from	effects	1721:1727	arg1	structure					1785:1793	microbiota community structure	1764:1793	microbiota community structure	1764:1793	Thus, to obtain better functional outcome for sulfated sea cucumber polysaccharides and sea cucumber, more attention needs to be paid to the effects of inter-individual differences in microbiota community structure.
34507721	5	83	theme	Bacteroides	955:965	arg1	species					967:973	Bacteroides species	955:973	Bacteroides species	955:973	Further in vitro fermentation of SCSPsj with 10 Parabacteroides and Bacteroides species suggests that Parabacteroides distasonis, enriched in Sample C, plays a critical role in the utilization of the polysaccharides.
34507721	2	84	theme	different	417:425	arg1	donors					427:432	different donors	417:432	different donors	417:432	The present study compared the responses of gut microbiota communities from different donors to SCSPsj, and the key bacteria were identified by 16S rRNA gene sequencing analysis and in vitro fermentation with specific bacteria.
34507721	5	85	dep	in	895:896	arg1	vitro					898:902	vitro	898:902	vitro	898:902	Further in vitro fermentation of SCSPsj with 10 Parabacteroides and Bacteroides species suggests that Parabacteroides distasonis, enriched in Sample C, plays a critical role in the utilization of the polysaccharides.
34507721	2	86	theme	communities	400:410	arg1	responses					372:380	the responses	368:380	the responses of gut microbiota communities from different donors to SCSPsj	368:442	The present study compared the responses of gut microbiota communities from different donors to SCSPsj, and the key bacteria were identified by 16S rRNA gene sequencing analysis and in vitro fermentation with specific bacteria.
34507721	0	87	theme	sea	36:38	arg1	polysaccharides					49:63	sulfated sea cucumber polysaccharides	27:63	sulfated sea cucumber polysaccharides	27:63	Gut microbiota response to sulfated sea cucumber polysaccharides in a differential manner using an in vitro fermentation model.
34507721	4	88	theme	utilization	790:800	arg1	capability					802:811	the relatively strong SCSPsj utilization capability	761:811	the relatively strong SCSPsj utilization capability	761:811	Further comparison of Samples A and C demonstrated that Sample C with the relatively strong SCSPsj utilization capability possessed more Parabacteroides while Sample A contained more Bacteroides.
34507721	2	89	theme	gut	385:387	arg1	communities					400:410	gut microbiota communities	385:410	gut microbiota communities from different donors	385:432	The present study compared the responses of gut microbiota communities from different donors to SCSPsj, and the key bacteria were identified by 16S rRNA gene sequencing analysis and in vitro fermentation with specific bacteria.
34507721	1	90	theme	host	286:289	arg1	health					291:296	the host health	282:296	the host health	282:296	Sea cucumber Stichopus japonicus has been consumed as high-valued seafood in Asian, and its sulfated polysaccharide (SCSPsj) has been inferred to benefit the host health via modulating gut microbiota composition.
34507721	3	91	theme	different	650:658	arg1	degrees					660:666	different degrees	650:666	different degrees in vitro fermentation	650:688	Gut microbiota communities from 6 donors (A ~ F) utilized the polysaccharides to different degrees in vitro fermentation.
34507721	2	92	from	responses	372:380	arg1	donors					427:432	different donors	417:432	different donors	417:432	The present study compared the responses of gut microbiota communities from different donors to SCSPsj, and the key bacteria were identified by 16S rRNA gene sequencing analysis and in vitro fermentation with specific bacteria.
34507721	6	93	theme	fatty	1126:1130	arg1	acids					1132:1136	short chain fatty acids	1114:1136	short chain fatty acids	1114:1136	Moreover, short chain fatty acids and the metabolite profiles of Samples A and C were also compared, and the results showed that more beneficial metabolites were accumulated by the microbiota community consuming more sulfated sea cucumber polysaccharides.
34507721	8	94	theme	better	1596:1601	arg1	outcome					1614:1620	better functional outcome	1596:1620	better functional outcome	1596:1620	Thus, to obtain better functional outcome for sulfated sea cucumber polysaccharides and sea cucumber, more attention needs to be paid to the effects of inter-individual differences in microbiota community structure.
34507721	6	95	theme	short	1114:1118	arg1	acids					1132:1136	short chain fatty acids	1114:1136	short chain fatty acids	1114:1136	Moreover, short chain fatty acids and the metabolite profiles of Samples A and C were also compared, and the results showed that more beneficial metabolites were accumulated by the microbiota community consuming more sulfated sea cucumber polysaccharides.
34507721	4	96	theme	strong	776:781	arg1	capability					802:811	the relatively strong SCSPsj utilization capability	761:811	the relatively strong SCSPsj utilization capability	761:811	Further comparison of Samples A and C demonstrated that Sample C with the relatively strong SCSPsj utilization capability possessed more Parabacteroides while Sample A contained more Bacteroides.
34507721	2	97	theme	present	345:351	arg1	study					353:357	The present study	341:357	The present study	341:357	The present study compared the responses of gut microbiota communities from different donors to SCSPsj, and the key bacteria were identified by 16S rRNA gene sequencing analysis and in vitro fermentation with specific bacteria.
32027903	5	0	theme	order	738:742	arg1	kinetics					744:751	pseudo-second order kinetics	724:751	pseudo-second order kinetics model	724:757	Non-linear kinetic and equilibrium isotherm employed and dye adsorption data fitted well to pseudo-second order kinetics model.
32027903	11	1	theme	effluents	1561:1569	arg1	treatment					1540:1548	the treatment	1536:1548	the treatment of textile effluents	1536:1569	The results showed that cellulose/clay composite are efficient for the removal dyes and could possibly be used for the treatment of textile effluents.
32027903	7	2	theme	Thermodynamic	866:878	arg1	ΔG°					902:904	ΔG°	902:904	ΔG°	902:904	Thermodynamic factors (ΔS°, ΔH° and ΔG°) revealed that the sorption process was spontaneous, exothermic and feasible.
32027903	7	2	theme	Thermodynamic	866:878	arg1	ΔH°					894:896	ΔH°	894:896	ΔH°	894:896	Thermodynamic factors (ΔS°, ΔH° and ΔG°) revealed that the sorption process was spontaneous, exothermic and feasible.
32027903	7	2	theme	Thermodynamic	866:878	arg1	ΔS°					889:891	ΔS°	889:891	ΔS°	889:891	Thermodynamic factors (ΔS°, ΔH° and ΔG°) revealed that the sorption process was spontaneous, exothermic and feasible.
32027903	7	2	theme	Thermodynamic	866:878	arg1	factors					880:886	Thermodynamic factors	866:886	Thermodynamic factors (ΔS°, ΔH° and ΔG°)	866:905	Thermodynamic factors (ΔS°, ΔH° and ΔG°) revealed that the sorption process was spontaneous, exothermic and feasible.
32027903	8	3	from	pH 2	1063:1066	arg1	dye					1044:1046	89.95% dye	1037:1046	89.95% dye with 60 min at pH 2 and 30 °C	1037:1076	Cellulose/clay composite I and II removed 88.64% and 89.95% dye with 60 min at pH 2 and 30 °C, respectively.
32027903	8	3	from	pH 2	1063:1066	arg1	%					1031:1031	88.64%	1026:1031	88.64%	1026:1031	Cellulose/clay composite I and II removed 88.64% and 89.95% dye with 60 min at pH 2 and 30 °C, respectively.
32027903	5	4	theme	kinetics	744:751	arg1	model					753:757	pseudo-second order kinetics model	724:757	pseudo-second order kinetics model	724:757	Non-linear kinetic and equilibrium isotherm employed and dye adsorption data fitted well to pseudo-second order kinetics model.
32027903	8	5	theme	Cellulose/clay	984:997	arg1	composite					999:1007	Cellulose/clay composite I and II	984:1016	Cellulose/clay composite I and II	984:1016	Cellulose/clay composite I and II removed 88.64% and 89.95% dye with 60 min at pH 2 and 30 °C, respectively.
32027903	7	6	dep	factors	880:886	arg1	ΔG°					902:904	ΔG°	902:904	ΔG°	902:904	Thermodynamic factors (ΔS°, ΔH° and ΔG°) revealed that the sorption process was spontaneous, exothermic and feasible.
32027903	7	6	dep	factors	880:886	arg1	ΔH°					894:896	ΔH°	894:896	ΔH°	894:896	Thermodynamic factors (ΔS°, ΔH° and ΔG°) revealed that the sorption process was spontaneous, exothermic and feasible.
32027903	7	6	dep	factors	880:886	arg1	ΔS°					889:891	ΔS°	889:891	ΔS°	889:891	Thermodynamic factors (ΔS°, ΔH° and ΔG°) revealed that the sorption process was spontaneous, exothermic and feasible.
32027903	7	6	dep	factors	880:886	arg1	factors					880:886	Thermodynamic factors	866:886	Thermodynamic factors (ΔS°, ΔH° and ΔG°)	866:905	Thermodynamic factors (ΔS°, ΔH° and ΔG°) revealed that the sorption process was spontaneous, exothermic and feasible.
32027903	9	7	theme	desorption	1189:1198	arg1	efficiency					1200:1209	higher desorption efficiency	1182:1209	higher desorption efficiency	1182:1209	For reusability, desorption was performed using different eluting agents and NaOH showed higher desorption efficiency.
32027903	5	8	theme	adsorption	693:702	arg1	data					704:707	dye adsorption data	689:707	dye adsorption data	689:707	Non-linear kinetic and equilibrium isotherm employed and dye adsorption data fitted well to pseudo-second order kinetics model.
32027903	10	9	dep	composite	1387:1395	arg1	II					1403:1404	II	1403:1404	II	1403:1404	For the treatment of wastewater, the developed composites were applied to textile effluents and color removal of (90-96.07%) and (97-98.23%) was achieved using cellulose/clay composite I and II, respectively.
32027903	10	9	dep	composite	1387:1395	arg1	I					1397:1397	I	1397:1397	I	1397:1397	For the treatment of wastewater, the developed composites were applied to textile effluents and color removal of (90-96.07%) and (97-98.23%) was achieved using cellulose/clay composite I and II, respectively.
32027903	6	10	theme	Redlich-Peterson	781:796	arg1	isotherm					798:805	the Redlich-Peterson isotherm	777:805	the Redlich-Peterson isotherm	777:805	Among isotherms, the Redlich-Peterson isotherm well defined the sorption process of dye on to composites.
32027903	4	11	theme	time	563:566	arg1	function					543:550	a function	541:550	a function of contact time, temperature, pH, initial dye concentration and composite dose	541:629	Adsorption efficiencies were compared of composite I and II as a function of contact time, temperature, pH, initial dye concentration and composite dose.
32027903	4	11	theme	time	563:566	arg1	efficiencies					489:500	Adsorption efficiencies	478:500	Adsorption efficiencies	478:500	Adsorption efficiencies were compared of composite I and II as a function of contact time, temperature, pH, initial dye concentration and composite dose.
32027903	0	12	theme	enhanced	90:97	arg1	removal					99:105	enhanced removal	90:105	enhanced removal of Drimarine Yellow HF-3GL dye	90:136	Development of new organic-inorganic, hybrid bionanocomposite from cellulose and clay for enhanced removal of Drimarine Yellow HF-3GL dye.
32027903	11	13	theme	cellulose/clay	1445:1458	arg1	composite					1460:1468	cellulose/clay composite	1445:1468	cellulose/clay composite	1445:1468	The results showed that cellulose/clay composite are efficient for the removal dyes and could possibly be used for the treatment of textile effluents.
32027903	2	14	theme	FTIR	295:298	arg1	techniques					323:332	FTIR, TGA, EDX, SEM and XRD techniques	295:332	FTIR, TGA, EDX, SEM and XRD techniques	295:332	The prepared composites were characterized by FTIR, TGA, EDX, SEM and XRD techniques.
32027903	11	15	theme	textile	1553:1559	arg1	effluents					1561:1569	textile effluents	1553:1569	textile effluents	1553:1569	The results showed that cellulose/clay composite are efficient for the removal dyes and could possibly be used for the treatment of textile effluents.
32027903	4	16	theme	contact	555:561	arg1	time					563:566	contact time	555:566	contact time	555:566	Adsorption efficiencies were compared of composite I and II as a function of contact time, temperature, pH, initial dye concentration and composite dose.
32027903	0	17	theme	Drimarine	110:118	arg1	dye					134:136	Drimarine Yellow HF-3GL dye	110:136	Drimarine Yellow HF-3GL dye	110:136	Development of new organic-inorganic, hybrid bionanocomposite from cellulose and clay for enhanced removal of Drimarine Yellow HF-3GL dye.
32027903	10	18	theme	wastewater	1233:1242	arg1	treatment					1220:1228	the treatment	1216:1228	the treatment of wastewater	1216:1242	For the treatment of wastewater, the developed composites were applied to textile effluents and color removal of (90-96.07%) and (97-98.23%) was achieved using cellulose/clay composite I and II, respectively.
32027903	3	19	theme	local	404:408	arg1	source					410:415	local source	404:415	local source	404:415	Bagasse was used as a cellulose source, while clay was obtained from local source, which was modified chemically before composite preparation.
32027903	5	20	dep	kinetic	643:649	arg1	isotherm					667:674	isotherm	667:674	isotherm	667:674	Non-linear kinetic and equilibrium isotherm employed and dye adsorption data fitted well to pseudo-second order kinetics model.
32027903	9	21	theme	different	1141:1149	arg1	agents					1159:1164	different eluting agents	1141:1164	different eluting agents	1141:1164	For reusability, desorption was performed using different eluting agents and NaOH showed higher desorption efficiency.
32027903	9	22	theme	higher	1182:1187	arg1	efficiency					1200:1209	higher desorption efficiency	1182:1209	higher desorption efficiency	1182:1209	For reusability, desorption was performed using different eluting agents and NaOH showed higher desorption efficiency.
32027903	8	23	dep	composite	999:1007	arg1	I					1009:1009	I	1009:1009	I	1009:1009	Cellulose/clay composite I and II removed 88.64% and 89.95% dye with 60 min at pH 2 and 30 °C, respectively.
32027903	8	23	dep	composite	999:1007	arg1	II					1015:1016	II	1015:1016	II	1015:1016	Cellulose/clay composite I and II removed 88.64% and 89.95% dye with 60 min at pH 2 and 30 °C, respectively.
32027903	3	24	theme	cellulose	357:365	arg1	Bagasse					335:341	Bagasse	335:341	Bagasse	335:341	Bagasse was used as a cellulose source, while clay was obtained from local source, which was modified chemically before composite preparation.
32027903	3	24	theme	cellulose	357:365	arg1	source					367:372	a cellulose source	355:372	a cellulose source	355:372	Bagasse was used as a cellulose source, while clay was obtained from local source, which was modified chemically before composite preparation.
32027903	3	25	used	used	347:350	arg2	Bagasse					335:341	Bagasse	335:341	Bagasse	335:341	Bagasse was used as a cellulose source, while clay was obtained from local source, which was modified chemically before composite preparation.
32027903	3	25	used	used	347:350	arg2	source					367:372	a cellulose source	355:372	a cellulose source	355:372	Bagasse was used as a cellulose source, while clay was obtained from local source, which was modified chemically before composite preparation.
32027903	9	26	theme	eluting	1151:1157	arg1	agents					1159:1164	different eluting agents	1141:1164	different eluting agents	1141:1164	For reusability, desorption was performed using different eluting agents and NaOH showed higher desorption efficiency.
32027903	4	27	theme	concentration	598:610	arg1	function					543:550	a function	541:550	a function of contact time, temperature, pH, initial dye concentration and composite dose	541:629	Adsorption efficiencies were compared of composite I and II as a function of contact time, temperature, pH, initial dye concentration and composite dose.
32027903	4	27	theme	concentration	598:610	arg1	efficiencies					489:500	Adsorption efficiencies	478:500	Adsorption efficiencies	478:500	Adsorption efficiencies were compared of composite I and II as a function of contact time, temperature, pH, initial dye concentration and composite dose.
32027903	10	28	theme	cellulose/clay	1372:1385	arg1	composite					1387:1395	cellulose/clay composite I and II	1372:1404	cellulose/clay composite I and II	1372:1404	For the treatment of wastewater, the developed composites were applied to textile effluents and color removal of (90-96.07%) and (97-98.23%) was achieved using cellulose/clay composite I and II, respectively.
32027903	2	29	theme	EDX	306:308	arg1	techniques					323:332	FTIR, TGA, EDX, SEM and XRD techniques	295:332	FTIR, TGA, EDX, SEM and XRD techniques	295:332	The prepared composites were characterized by FTIR, TGA, EDX, SEM and XRD techniques.
32027903	8	30	with	%	1031:1031	arg1	60 min					1053:1058	60 min	1053:1058	60 min	1053:1058	Cellulose/clay composite I and II removed 88.64% and 89.95% dye with 60 min at pH 2 and 30 °C, respectively.
32027903	4	31	theme	dose	626:629	arg1	function					543:550	a function	541:550	a function of contact time, temperature, pH, initial dye concentration and composite dose	541:629	Adsorption efficiencies were compared of composite I and II as a function of contact time, temperature, pH, initial dye concentration and composite dose.
32027903	4	31	theme	dose	626:629	arg1	efficiencies					489:500	Adsorption efficiencies	478:500	Adsorption efficiencies	478:500	Adsorption efficiencies were compared of composite I and II as a function of contact time, temperature, pH, initial dye concentration and composite dose.
32027903	0	32	theme	organic-inorganic	19:35	arg1	Development					0:10	Development	0:10	Development of new organic-inorganic, hybrid bionanocomposite from cellulose and clay for enhanced removal of Drimarine Yellow HF-3GL dye.	0:137	Development of new organic-inorganic, hybrid bionanocomposite from cellulose and clay for enhanced removal of Drimarine Yellow HF-3GL dye.
32027903	0	33	theme	HF-3GL	127:132	arg1	dye					134:136	Drimarine Yellow HF-3GL dye	110:136	Drimarine Yellow HF-3GL dye	110:136	Development of new organic-inorganic, hybrid bionanocomposite from cellulose and clay for enhanced removal of Drimarine Yellow HF-3GL dye.
32027903	10	34	theme	and	1337:1339	arg1	removal					1314:1320	color removal	1308:1320	color removal of (90-96.07%) and (97-98.23%)	1308:1351	For the treatment of wastewater, the developed composites were applied to textile effluents and color removal of (90-96.07%) and (97-98.23%) was achieved using cellulose/clay composite I and II, respectively.
32027903	4	35	theme	initial	586:592	arg1	concentration					598:610	initial dye concentration	586:610	initial dye concentration	586:610	Adsorption efficiencies were compared of composite I and II as a function of contact time, temperature, pH, initial dye concentration and composite dose.
32027903	0	36	theme	Yellow	120:125	arg1	dye					134:136	Drimarine Yellow HF-3GL dye	110:136	Drimarine Yellow HF-3GL dye	110:136	Development of new organic-inorganic, hybrid bionanocomposite from cellulose and clay for enhanced removal of Drimarine Yellow HF-3GL dye.
32027903	2	37	theme	TGA	301:303	arg1	techniques					323:332	FTIR, TGA, EDX, SEM and XRD techniques	295:332	FTIR, TGA, EDX, SEM and XRD techniques	295:332	The prepared composites were characterized by FTIR, TGA, EDX, SEM and XRD techniques.
32027903	10	38	theme	developed	1249:1257	arg1	composites					1259:1268	the developed composites	1245:1268	the developed composites	1245:1268	For the treatment of wastewater, the developed composites were applied to textile effluents and color removal of (90-96.07%) and (97-98.23%) was achieved using cellulose/clay composite I and II, respectively.
32027903	11	39	used	used	1527:1530	arg2	composite					1460:1468	cellulose/clay composite	1445:1468	cellulose/clay composite	1445:1468	The results showed that cellulose/clay composite are efficient for the removal dyes and could possibly be used for the treatment of textile effluents.
32027903	0	40	theme	hybrid	38:43	arg1	organic-inorganic					19:35	organic-inorganic	19:35	organic-inorganic	19:35	Development of new organic-inorganic, hybrid bionanocomposite from cellulose and clay for enhanced removal of Drimarine Yellow HF-3GL dye.
32027903	0	40	theme	hybrid	38:43	arg1	bionanocomposite					45:60	hybrid bionanocomposite	38:60	hybrid bionanocomposite from cellulose and clay for enhanced removal of Drimarine Yellow HF-3GL dye	38:136	Development of new organic-inorganic, hybrid bionanocomposite from cellulose and clay for enhanced removal of Drimarine Yellow HF-3GL dye.
32027903	0	41	from	clay	81:84	arg1	organic-inorganic					19:35	organic-inorganic	19:35	organic-inorganic	19:35	Development of new organic-inorganic, hybrid bionanocomposite from cellulose and clay for enhanced removal of Drimarine Yellow HF-3GL dye.
32027903	0	41	from	clay	81:84	arg1	bionanocomposite					45:60	hybrid bionanocomposite	38:60	hybrid bionanocomposite from cellulose and clay for enhanced removal of Drimarine Yellow HF-3GL dye	38:136	Development of new organic-inorganic, hybrid bionanocomposite from cellulose and clay for enhanced removal of Drimarine Yellow HF-3GL dye.
32027903	1	42	theme	Drimarine	213:221	arg1	dye					244:246	Drimarine Yellow HF-3GL direct dye	213:246	Drimarine Yellow HF-3GL direct dye	213:246	Cellulose/clay composites were prepared and applied for the adsorption of Drimarine Yellow HF-3GL direct dye.
32027903	4	43	theme	composite	616:624	arg1	dose					626:629	composite dose	616:629	composite dose	616:629	Adsorption efficiencies were compared of composite I and II as a function of contact time, temperature, pH, initial dye concentration and composite dose.
32027903	0	44	theme	dye	134:136	arg1	removal					99:105	enhanced removal	90:105	enhanced removal of Drimarine Yellow HF-3GL dye	90:136	Development of new organic-inorganic, hybrid bionanocomposite from cellulose and clay for enhanced removal of Drimarine Yellow HF-3GL dye.
32027903	8	45	from	30 °C	1072:1076	arg1	dye					1044:1046	89.95% dye	1037:1046	89.95% dye with 60 min at pH 2 and 30 °C	1037:1076	Cellulose/clay composite I and II removed 88.64% and 89.95% dye with 60 min at pH 2 and 30 °C, respectively.
32027903	8	45	from	30 °C	1072:1076	arg1	%					1031:1031	88.64%	1026:1031	88.64%	1026:1031	Cellulose/clay composite I and II removed 88.64% and 89.95% dye with 60 min at pH 2 and 30 °C, respectively.
32027903	4	46	theme	pH	582:583	arg1	function					543:550	a function	541:550	a function of contact time, temperature, pH, initial dye concentration and composite dose	541:629	Adsorption efficiencies were compared of composite I and II as a function of contact time, temperature, pH, initial dye concentration and composite dose.
32027903	4	46	theme	pH	582:583	arg1	efficiencies					489:500	Adsorption efficiencies	478:500	Adsorption efficiencies	478:500	Adsorption efficiencies were compared of composite I and II as a function of contact time, temperature, pH, initial dye concentration and composite dose.
32027903	1	47	theme	Yellow	223:228	arg1	dye					244:246	Drimarine Yellow HF-3GL direct dye	213:246	Drimarine Yellow HF-3GL direct dye	213:246	Cellulose/clay composites were prepared and applied for the adsorption of Drimarine Yellow HF-3GL direct dye.
32027903	0	48	from	cellulose	67:75	arg1	organic-inorganic					19:35	organic-inorganic	19:35	organic-inorganic	19:35	Development of new organic-inorganic, hybrid bionanocomposite from cellulose and clay for enhanced removal of Drimarine Yellow HF-3GL dye.
32027903	0	48	from	cellulose	67:75	arg1	bionanocomposite					45:60	hybrid bionanocomposite	38:60	hybrid bionanocomposite from cellulose and clay for enhanced removal of Drimarine Yellow HF-3GL dye	38:136	Development of new organic-inorganic, hybrid bionanocomposite from cellulose and clay for enhanced removal of Drimarine Yellow HF-3GL dye.
32027903	10	49	theme	textile	1286:1292	arg1	effluents					1294:1302	textile effluents	1286:1302	textile effluents	1286:1302	For the treatment of wastewater, the developed composites were applied to textile effluents and color removal of (90-96.07%) and (97-98.23%) was achieved using cellulose/clay composite I and II, respectively.
32027903	3	50	theme	composite	455:463	arg1	preparation					465:475	composite preparation	455:475	composite preparation	455:475	Bagasse was used as a cellulose source, while clay was obtained from local source, which was modified chemically before composite preparation.
32027903	5	51	theme	dye	689:691	arg1	data					704:707	dye adsorption data	689:707	dye adsorption data	689:707	Non-linear kinetic and equilibrium isotherm employed and dye adsorption data fitted well to pseudo-second order kinetics model.
32027903	8	52	theme	89.95	1037:1041	arg1	%					1042:1042	%	1042:1042	%	1042:1042	Cellulose/clay composite I and II removed 88.64% and 89.95% dye with 60 min at pH 2 and 30 °C, respectively.
32027903	6	53	theme	sorption	824:831	arg1	process					833:839	the sorption process	820:839	the sorption process of dye	820:846	Among isotherms, the Redlich-Peterson isotherm well defined the sorption process of dye on to composites.
32027903	2	54	theme	XRD	319:321	arg1	techniques					323:332	FTIR, TGA, EDX, SEM and XRD techniques	295:332	FTIR, TGA, EDX, SEM and XRD techniques	295:332	The prepared composites were characterized by FTIR, TGA, EDX, SEM and XRD techniques.
32027903	8	55	with	dye	1044:1046	arg1	60 min					1053:1058	60 min	1053:1058	60 min	1053:1058	Cellulose/clay composite I and II removed 88.64% and 89.95% dye with 60 min at pH 2 and 30 °C, respectively.
32027903	11	56	theme	removal	1492:1498	arg1	dyes					1500:1503	the removal dyes	1488:1503	the removal dyes	1488:1503	The results showed that cellulose/clay composite are efficient for the removal dyes and could possibly be used for the treatment of textile effluents.
32027903	7	57	theme	sorption	925:932	arg1	spontaneous					946:956	spontaneous	946:956	spontaneous	946:956	Thermodynamic factors (ΔS°, ΔH° and ΔG°) revealed that the sorption process was spontaneous, exothermic and feasible.
32027903	7	57	theme	sorption	925:932	arg1	process					934:940	the sorption process	921:940	the sorption process	921:940	Thermodynamic factors (ΔS°, ΔH° and ΔG°) revealed that the sorption process was spontaneous, exothermic and feasible.
32027903	1	58	theme	HF-3GL	230:235	arg1	dye					244:246	Drimarine Yellow HF-3GL direct dye	213:246	Drimarine Yellow HF-3GL direct dye	213:246	Cellulose/clay composites were prepared and applied for the adsorption of Drimarine Yellow HF-3GL direct dye.
32027903	2	59	theme	SEM	311:313	arg1	techniques					323:332	FTIR, TGA, EDX, SEM and XRD techniques	295:332	FTIR, TGA, EDX, SEM and XRD techniques	295:332	The prepared composites were characterized by FTIR, TGA, EDX, SEM and XRD techniques.
32027903	1	60	theme	direct	237:242	arg1	dye					244:246	Drimarine Yellow HF-3GL direct dye	213:246	Drimarine Yellow HF-3GL direct dye	213:246	Cellulose/clay composites were prepared and applied for the adsorption of Drimarine Yellow HF-3GL direct dye.
32027903	6	61	theme	dye	844:846	arg1	process					833:839	the sorption process	820:839	the sorption process of dye	820:846	Among isotherms, the Redlich-Peterson isotherm well defined the sorption process of dye on to composites.
32027903	4	62	theme	dye	594:596	arg1	concentration					598:610	initial dye concentration	586:610	initial dye concentration	586:610	Adsorption efficiencies were compared of composite I and II as a function of contact time, temperature, pH, initial dye concentration and composite dose.
32027903	2	63	theme	prepared	253:260	arg1	composites					262:271	The prepared composites	249:271	The prepared composites	249:271	The prepared composites were characterized by FTIR, TGA, EDX, SEM and XRD techniques.
32027903	1	64	theme	Cellulose/clay	139:152	arg1	composites					154:163	Cellulose/clay composites	139:163	Cellulose/clay composites	139:163	Cellulose/clay composites were prepared and applied for the adsorption of Drimarine Yellow HF-3GL direct dye.
32027903	1	65	theme	dye	244:246	arg1	adsorption					199:208	the adsorption	195:208	the adsorption of Drimarine Yellow HF-3GL direct dye	195:246	Cellulose/clay composites were prepared and applied for the adsorption of Drimarine Yellow HF-3GL direct dye.
32027903	5	66	theme	pseudo-second	724:736	arg1	kinetics					744:751	pseudo-second order kinetics	724:751	pseudo-second order kinetics model	724:757	Non-linear kinetic and equilibrium isotherm employed and dye adsorption data fitted well to pseudo-second order kinetics model.
32027903	4	67	theme	Adsorption	478:487	arg1	function					543:550	a function	541:550	a function of contact time, temperature, pH, initial dye concentration and composite dose	541:629	Adsorption efficiencies were compared of composite I and II as a function of contact time, temperature, pH, initial dye concentration and composite dose.
32027903	4	67	theme	Adsorption	478:487	arg1	efficiencies					489:500	Adsorption efficiencies	478:500	Adsorption efficiencies	478:500	Adsorption efficiencies were compared of composite I and II as a function of contact time, temperature, pH, initial dye concentration and composite dose.
32027903	10	68	theme	color	1308:1312	arg1	removal					1314:1320	color removal	1308:1320	color removal of (90-96.07%) and (97-98.23%)	1308:1351	For the treatment of wastewater, the developed composites were applied to textile effluents and color removal of (90-96.07%) and (97-98.23%) was achieved using cellulose/clay composite I and II, respectively.
32027903	4	69	theme	temperature	569:579	arg1	function					543:550	a function	541:550	a function of contact time, temperature, pH, initial dye concentration and composite dose	541:629	Adsorption efficiencies were compared of composite I and II as a function of contact time, temperature, pH, initial dye concentration and composite dose.
32027903	4	69	theme	temperature	569:579	arg1	efficiencies					489:500	Adsorption efficiencies	478:500	Adsorption efficiencies	478:500	Adsorption efficiencies were compared of composite I and II as a function of contact time, temperature, pH, initial dye concentration and composite dose.
32027903	8	70	theme	%	1042:1042	arg1	dye					1044:1046	89.95% dye	1037:1046	89.95% dye with 60 min at pH 2 and 30 °C	1037:1076	Cellulose/clay composite I and II removed 88.64% and 89.95% dye with 60 min at pH 2 and 30 °C, respectively.
33636274	0	0	theme	chitosan/oxidized-chitin	85:108	arg1	composites					123:132	chitosan/oxidized-chitin nanocrystals composites	85:132	chitosan/oxidized-chitin nanocrystals composites for real-time hairtail and shrimp freshness monitoring	85:187	Fabrication of halochromic smart films by immobilizing red cabbage anthocyanins into chitosan/oxidized-chitin nanocrystals composites for real-time hairtail and shrimp freshness monitoring.
33636274	4	1	theme	water	720:724	arg1	WVP					746:748	WVP	746:748	WVP	746:748	When the proper concentration of RCAs was incorporated into the composites, improved water vapor permeability (WVP), oxygen permeability (OP), mechanical, UV-blocking, and antioxidant properties were observed.
33636274	4	1	theme	water	720:724	arg1	permeability					732:743	improved water vapor permeability	711:743	improved water vapor permeability (WVP)	711:749	When the proper concentration of RCAs was incorporated into the composites, improved water vapor permeability (WVP), oxygen permeability (OP), mechanical, UV-blocking, and antioxidant properties were observed.
33636274	4	1	theme	water	720:724	arg1	composites					699:708	the composites	695:708	the composites	695:708	When the proper concentration of RCAs was incorporated into the composites, improved water vapor permeability (WVP), oxygen permeability (OP), mechanical, UV-blocking, and antioxidant properties were observed.
33636274	3	2	theme	nanocrystals	537:548	arg1	composites					556:565	chitosan (CS)/oxidized-chitin nanocrystals (OCN) composites	507:565	chitosan (CS)/oxidized-chitin nanocrystals (OCN) composites	507:565	RCAs were successfully immobilized into chitosan (CS)/oxidized-chitin nanocrystals (OCN) composites through hydrogen bonding, and cohesive film structures were formed.
33636274	7	3	theme	storage	1206:1212	arg1	time					1214:1217	storage time	1206:1217	storage time	1206:1217	The film color changed significantly during storage time, and three stages of product freshness (fresh, medium fresh, and spoiled) were successfully differentiated.
33636274	6	4	theme	hairtail	1100:1107	arg1	freshness					1087:1095	the freshness	1083:1095	the freshness of hairtail (Trichiurus lepturus) and shrimp (Penaeus vannamei)	1083:1159	Finally, the smart films were applied to monitor the freshness of hairtail (Trichiurus lepturus) and shrimp (Penaeus vannamei).
33636274	4	5	theme	vapor	726:730	arg1	WVP					746:748	WVP	746:748	WVP	746:748	When the proper concentration of RCAs was incorporated into the composites, improved water vapor permeability (WVP), oxygen permeability (OP), mechanical, UV-blocking, and antioxidant properties were observed.
33636274	4	5	theme	vapor	726:730	arg1	permeability					732:743	improved water vapor permeability	711:743	improved water vapor permeability (WVP)	711:749	When the proper concentration of RCAs was incorporated into the composites, improved water vapor permeability (WVP), oxygen permeability (OP), mechanical, UV-blocking, and antioxidant properties were observed.
33636274	4	5	theme	vapor	726:730	arg1	composites					699:708	the composites	695:708	the composites	695:708	When the proper concentration of RCAs was incorporated into the composites, improved water vapor permeability (WVP), oxygen permeability (OP), mechanical, UV-blocking, and antioxidant properties were observed.
33636274	8	6	theme	freshness	1359:1367	arg1	indicators					1369:1378	three freshness indicators	1353:1378	three freshness indicators	1353:1378	Strong correlations among three freshness indicators and two colorimetric parameters were also identified and analyzed.
33636274	7	7	dep	freshness	1248:1256	arg1	fresh					1259:1263	fresh	1259:1263	fresh	1259:1263	The film color changed significantly during storage time, and three stages of product freshness (fresh, medium fresh, and spoiled) were successfully differentiated.
33636274	7	7	dep	freshness	1248:1256	arg1	fresh					1273:1277	fresh	1273:1277	fresh	1273:1277	The film color changed significantly during storage time, and three stages of product freshness (fresh, medium fresh, and spoiled) were successfully differentiated.
33636274	7	7	dep	freshness	1248:1256	arg1	spoiled					1284:1290	spoiled	1284:1290	spoiled	1284:1290	The film color changed significantly during storage time, and three stages of product freshness (fresh, medium fresh, and spoiled) were successfully differentiated.
33636274	3	8	theme	OCN	551:553	arg1	composites					556:565	chitosan (CS)/oxidized-chitin nanocrystals (OCN) composites	507:565	chitosan (CS)/oxidized-chitin nanocrystals (OCN) composites	507:565	RCAs were successfully immobilized into chitosan (CS)/oxidized-chitin nanocrystals (OCN) composites through hydrogen bonding, and cohesive film structures were formed.
33636274	3	9	theme	hydrogen	575:582	arg1	bonding					584:590	hydrogen bonding	575:590	hydrogen bonding	575:590	RCAs were successfully immobilized into chitosan (CS)/oxidized-chitin nanocrystals (OCN) composites through hydrogen bonding, and cohesive film structures were formed.
33636274	6	10	theme	shrimp	1135:1140	arg1	freshness					1087:1095	the freshness	1083:1095	the freshness of hairtail (Trichiurus lepturus) and shrimp (Penaeus vannamei)	1083:1159	Finally, the smart films were applied to monitor the freshness of hairtail (Trichiurus lepturus) and shrimp (Penaeus vannamei).
33636274	0	11	theme	nanocrystals	110:121	arg1	composites					123:132	chitosan/oxidized-chitin nanocrystals composites	85:132	chitosan/oxidized-chitin nanocrystals composites for real-time hairtail and shrimp freshness monitoring	85:187	Fabrication of halochromic smart films by immobilizing red cabbage anthocyanins into chitosan/oxidized-chitin nanocrystals composites for real-time hairtail and shrimp freshness monitoring.
33636274	6	12	theme	Penaeus	1143:1149	arg1	shrimp					1135:1140	shrimp	1135:1140	shrimp (Penaeus vannamei)	1135:1159	Finally, the smart films were applied to monitor the freshness of hairtail (Trichiurus lepturus) and shrimp (Penaeus vannamei).
33636274	6	12	theme	Penaeus	1143:1149	arg1	vannamei					1151:1158	Penaeus vannamei	1143:1158	Penaeus vannamei	1143:1158	Finally, the smart films were applied to monitor the freshness of hairtail (Trichiurus lepturus) and shrimp (Penaeus vannamei).
33636274	9	13	theme	biodegradable	1502:1514	arg1	components					1516:1525	non-toxic and biodegradable components	1488:1525	non-toxic and biodegradable components	1488:1525	Overall, the smart system assembled from non-toxic and biodegradable components could contribute to monitoring the freshness of seafood, like hairtail and shrimp, in real-time.
33636274	0	14	theme	real-time	138:146	arg1	hairtail					148:155	real-time hairtail and shrimp freshness monitoring	138:187	hairtail	148:155	Fabrication of halochromic smart films by immobilizing red cabbage anthocyanins into chitosan/oxidized-chitin nanocrystals composites for real-time hairtail and shrimp freshness monitoring.
33636274	4	15	theme	oxygen	752:757	arg1	OP					773:774	OP	773:774	OP	773:774	When the proper concentration of RCAs was incorporated into the composites, improved water vapor permeability (WVP), oxygen permeability (OP), mechanical, UV-blocking, and antioxidant properties were observed.
33636274	4	15	theme	oxygen	752:757	arg1	composites					699:708	the composites	695:708	the composites	695:708	When the proper concentration of RCAs was incorporated into the composites, improved water vapor permeability (WVP), oxygen permeability (OP), mechanical, UV-blocking, and antioxidant properties were observed.
33636274	4	15	theme	oxygen	752:757	arg1	permeability					759:770	oxygen permeability	752:770	oxygen permeability (OP)	752:775	When the proper concentration of RCAs was incorporated into the composites, improved water vapor permeability (WVP), oxygen permeability (OP), mechanical, UV-blocking, and antioxidant properties were observed.
33636274	5	16	theme	short	970:974	arg1	intervals					981:989	short time intervals	970:989	short time intervals	970:989	Moreover, the smart films exhibited distinguishable changes of color to ammonia vapor and acidic/alkaline environment within short time intervals, which were easy to discern by naked eyes.
33636274	3	17	theme	chitosan	507:514	arg1	composites					556:565	chitosan (CS)/oxidized-chitin nanocrystals (OCN) composites	507:565	chitosan (CS)/oxidized-chitin nanocrystals (OCN) composites	507:565	RCAs were successfully immobilized into chitosan (CS)/oxidized-chitin nanocrystals (OCN) composites through hydrogen bonding, and cohesive film structures were formed.
33636274	5	18	theme	distinguishable	881:895	arg1	changes					897:903	distinguishable changes	881:903	distinguishable changes of color to ammonia vapor and acidic/alkaline environment	881:961	Moreover, the smart films exhibited distinguishable changes of color to ammonia vapor and acidic/alkaline environment within short time intervals, which were easy to discern by naked eyes.
33636274	4	19	theme	mechanical	778:787	arg1	properties					819:828	mechanical, UV-blocking, and antioxidant properties	778:828	mechanical, UV-blocking, and antioxidant properties	778:828	When the proper concentration of RCAs was incorporated into the composites, improved water vapor permeability (WVP), oxygen permeability (OP), mechanical, UV-blocking, and antioxidant properties were observed.
33636274	7	20	theme	product	1240:1246	arg1	freshness					1248:1256	product freshness	1240:1256	product freshness (fresh, medium fresh, and spoiled)	1240:1291	The film color changed significantly during storage time, and three stages of product freshness (fresh, medium fresh, and spoiled) were successfully differentiated.
33636274	2	21	theme	Red	333:335	arg1	RCAs					359:362	RCAs	359:362	RCAs	359:362	Red cabbage anthocyanins (RCAs) solution illustrated significant color variations (red-pink-blue-green) in different pH environments.
33636274	2	21	theme	Red	333:335	arg1	anthocyanins					345:356	Red cabbage anthocyanins	333:356	Red cabbage anthocyanins (RCAs) solution	333:372	Red cabbage anthocyanins (RCAs) solution illustrated significant color variations (red-pink-blue-green) in different pH environments.
33636274	2	22	dep	anthocyanins	345:356	arg1	solution					365:372	solution	365:372	Red cabbage anthocyanins (RCAs) solution	333:372	Red cabbage anthocyanins (RCAs) solution illustrated significant color variations (red-pink-blue-green) in different pH environments.
33636274	1	23	theme	halochromic	205:215	arg1	films					223:227	halochromic smart films	205:227	halochromic smart films	205:227	In this study, halochromic smart films were produced, characterized, and applied to monitor the freshness of hairtail and shrimp in real-time.
33636274	7	24	theme	film	1166:1169	arg1	color					1171:1175	The film color	1162:1175	The film color	1162:1175	The film color changed significantly during storage time, and three stages of product freshness (fresh, medium fresh, and spoiled) were successfully differentiated.
33636274	9	25	theme	seafood	1575:1581	arg1	freshness					1562:1570	the freshness	1558:1570	the freshness of seafood	1558:1581	Overall, the smart system assembled from non-toxic and biodegradable components could contribute to monitoring the freshness of seafood, like hairtail and shrimp, in real-time.
33636274	3	26	theme	film	606:609	arg1	structures					611:620	cohesive film structures	597:620	cohesive film structures	597:620	RCAs were successfully immobilized into chitosan (CS)/oxidized-chitin nanocrystals (OCN) composites through hydrogen bonding, and cohesive film structures were formed.
33636274	2	27	theme	color	398:402	arg1	red-pink-blue-green					416:434	red-pink-blue-green	416:434	red-pink-blue-green	416:434	Red cabbage anthocyanins (RCAs) solution illustrated significant color variations (red-pink-blue-green) in different pH environments.
33636274	2	27	theme	color	398:402	arg1	variations					404:413	significant color variations	386:413	significant color variations (red-pink-blue-green) in different pH environments	386:464	Red cabbage anthocyanins (RCAs) solution illustrated significant color variations (red-pink-blue-green) in different pH environments.
33636274	0	28	theme	smart	27:31	arg1	films					33:37	halochromic smart films	15:37	halochromic smart films	15:37	Fabrication of halochromic smart films by immobilizing red cabbage anthocyanins into chitosan/oxidized-chitin nanocrystals composites for real-time hairtail and shrimp freshness monitoring.
33636274	1	29	theme	hairtail	299:306	arg1	freshness					286:294	the freshness	282:294	the freshness of hairtail and shrimp in real-time	282:330	In this study, halochromic smart films were produced, characterized, and applied to monitor the freshness of hairtail and shrimp in real-time.
33636274	8	30	theme	colorimetric	1388:1399	arg1	parameters					1401:1410	two colorimetric parameters	1384:1410	two colorimetric parameters	1384:1410	Strong correlations among three freshness indicators and two colorimetric parameters were also identified and analyzed.
33636274	5	31	theme	time	976:979	arg1	intervals					981:989	short time intervals	970:989	short time intervals	970:989	Moreover, the smart films exhibited distinguishable changes of color to ammonia vapor and acidic/alkaline environment within short time intervals, which were easy to discern by naked eyes.
33636274	2	32	theme	pH	450:451	arg1	environments					453:464	different pH environments	440:464	different pH environments	440:464	Red cabbage anthocyanins (RCAs) solution illustrated significant color variations (red-pink-blue-green) in different pH environments.
33636274	0	33	theme	halochromic	15:25	arg1	films					33:37	halochromic smart films	15:37	halochromic smart films	15:37	Fabrication of halochromic smart films by immobilizing red cabbage anthocyanins into chitosan/oxidized-chitin nanocrystals composites for real-time hairtail and shrimp freshness monitoring.
33636274	1	34	from	freshness	286:294	arg1	real-time					322:330	real-time	322:330	real-time	322:330	In this study, halochromic smart films were produced, characterized, and applied to monitor the freshness of hairtail and shrimp in real-time.
33636274	5	35	theme	ammonia	917:923	arg1	vapor					925:929	ammonia vapor	917:929	ammonia vapor	917:929	Moreover, the smart films exhibited distinguishable changes of color to ammonia vapor and acidic/alkaline environment within short time intervals, which were easy to discern by naked eyes.
33636274	2	36	theme	different	440:448	arg1	environments					453:464	different pH environments	440:464	different pH environments	440:464	Red cabbage anthocyanins (RCAs) solution illustrated significant color variations (red-pink-blue-green) in different pH environments.
33636274	1	37	theme	shrimp	312:317	arg1	freshness					286:294	the freshness	282:294	the freshness of hairtail and shrimp in real-time	282:330	In this study, halochromic smart films were produced, characterized, and applied to monitor the freshness of hairtail and shrimp in real-time.
33636274	0	38	theme	freshness	168:176	arg1	monitoring					178:187	real-time hairtail and shrimp freshness monitoring	138:187	monitoring	178:187	Fabrication of halochromic smart films by immobilizing red cabbage anthocyanins into chitosan/oxidized-chitin nanocrystals composites for real-time hairtail and shrimp freshness monitoring.
33636274	1	39	theme	smart	217:221	arg1	films					223:227	halochromic smart films	205:227	halochromic smart films	205:227	In this study, halochromic smart films were produced, characterized, and applied to monitor the freshness of hairtail and shrimp in real-time.
33636274	6	40	theme	smart	1047:1051	arg1	films					1053:1057	the smart films	1043:1057	the smart films	1043:1057	Finally, the smart films were applied to monitor the freshness of hairtail (Trichiurus lepturus) and shrimp (Penaeus vannamei).
33636274	0	41	theme	films	33:37	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of halochromic smart films by immobilizing red cabbage anthocyanins into chitosan/oxidized-chitin nanocrystals composites for real-time hairtail and shrimp freshness monitoring.	0:188	Fabrication of halochromic smart films by immobilizing red cabbage anthocyanins into chitosan/oxidized-chitin nanocrystals composites for real-time hairtail and shrimp freshness monitoring.
33636274	0	42	theme	shrimp	161:166	arg1	monitoring					178:187	real-time hairtail and shrimp freshness monitoring	138:187	monitoring	178:187	Fabrication of halochromic smart films by immobilizing red cabbage anthocyanins into chitosan/oxidized-chitin nanocrystals composites for real-time hairtail and shrimp freshness monitoring.
33636274	2	43	from	variations	404:413	arg1	environments					453:464	different pH environments	440:464	different pH environments	440:464	Red cabbage anthocyanins (RCAs) solution illustrated significant color variations (red-pink-blue-green) in different pH environments.
33636274	5	44	theme	smart	859:863	arg1	films					865:869	the smart films	855:869	the smart films	855:869	Moreover, the smart films exhibited distinguishable changes of color to ammonia vapor and acidic/alkaline environment within short time intervals, which were easy to discern by naked eyes.
33636274	0	45	theme	red	55:57	arg1	anthocyanins					67:78	red cabbage anthocyanins	55:78	red cabbage anthocyanins	55:78	Fabrication of halochromic smart films by immobilizing red cabbage anthocyanins into chitosan/oxidized-chitin nanocrystals composites for real-time hairtail and shrimp freshness monitoring.
33636274	9	46	theme	non-toxic	1488:1496	arg1	components					1516:1525	non-toxic and biodegradable components	1488:1525	non-toxic and biodegradable components	1488:1525	Overall, the smart system assembled from non-toxic and biodegradable components could contribute to monitoring the freshness of seafood, like hairtail and shrimp, in real-time.
33636274	6	47	dep	hairtail	1100:1107	arg1	lepturus					1121:1128	Trichiurus lepturus	1110:1128	Trichiurus lepturus	1110:1128	Finally, the smart films were applied to monitor the freshness of hairtail (Trichiurus lepturus) and shrimp (Penaeus vannamei).
33636274	9	48	theme	smart	1460:1464	arg1	system					1466:1471	the smart system	1456:1471	the smart system assembled from non-toxic and biodegradable components	1456:1525	Overall, the smart system assembled from non-toxic and biodegradable components could contribute to monitoring the freshness of seafood, like hairtail and shrimp, in real-time.
33636274	3	49	theme	/oxidized-chitin	520:535	arg1	composites					556:565	chitosan (CS)/oxidized-chitin nanocrystals (OCN) composites	507:565	chitosan (CS)/oxidized-chitin nanocrystals (OCN) composites	507:565	RCAs were successfully immobilized into chitosan (CS)/oxidized-chitin nanocrystals (OCN) composites through hydrogen bonding, and cohesive film structures were formed.
33636274	3	50	theme	cohesive	597:604	arg1	structures					611:620	cohesive film structures	597:620	cohesive film structures	597:620	RCAs were successfully immobilized into chitosan (CS)/oxidized-chitin nanocrystals (OCN) composites through hydrogen bonding, and cohesive film structures were formed.
33636274	5	51	theme	acidic/alkaline	935:949	arg1	environment					951:961	acidic/alkaline environment	935:961	acidic/alkaline environment	935:961	Moreover, the smart films exhibited distinguishable changes of color to ammonia vapor and acidic/alkaline environment within short time intervals, which were easy to discern by naked eyes.
33636274	8	52	theme	Strong	1327:1332	arg1	correlations					1334:1345	Strong correlations	1327:1345	Strong correlations among three freshness indicators and two colorimetric parameters	1327:1410	Strong correlations among three freshness indicators and two colorimetric parameters were also identified and analyzed.
33636274	4	53	theme	UV-blocking	790:800	arg1	properties					819:828	mechanical, UV-blocking, and antioxidant properties	778:828	mechanical, UV-blocking, and antioxidant properties	778:828	When the proper concentration of RCAs was incorporated into the composites, improved water vapor permeability (WVP), oxygen permeability (OP), mechanical, UV-blocking, and antioxidant properties were observed.
33636274	2	54	theme	cabbage	337:343	arg1	RCAs					359:362	RCAs	359:362	RCAs	359:362	Red cabbage anthocyanins (RCAs) solution illustrated significant color variations (red-pink-blue-green) in different pH environments.
33636274	2	54	theme	cabbage	337:343	arg1	anthocyanins					345:356	Red cabbage anthocyanins	333:356	Red cabbage anthocyanins (RCAs) solution	333:372	Red cabbage anthocyanins (RCAs) solution illustrated significant color variations (red-pink-blue-green) in different pH environments.
33636274	7	55	theme	freshness	1248:1256	arg1	stages					1230:1235	three stages	1224:1235	three stages of product freshness (fresh, medium fresh, and spoiled)	1224:1291	The film color changed significantly during storage time, and three stages of product freshness (fresh, medium fresh, and spoiled) were successfully differentiated.
33636274	5	56	theme	color	908:912	arg1	changes					897:903	distinguishable changes	881:903	distinguishable changes of color to ammonia vapor and acidic/alkaline environment	881:961	Moreover, the smart films exhibited distinguishable changes of color to ammonia vapor and acidic/alkaline environment within short time intervals, which were easy to discern by naked eyes.
33636274	4	57	theme	antioxidant	807:817	arg1	properties					819:828	mechanical, UV-blocking, and antioxidant properties	778:828	mechanical, UV-blocking, and antioxidant properties	778:828	When the proper concentration of RCAs was incorporated into the composites, improved water vapor permeability (WVP), oxygen permeability (OP), mechanical, UV-blocking, and antioxidant properties were observed.
33636274	4	58	theme	RCAs	668:671	arg1	concentration					651:663	the proper concentration	640:663	the proper concentration of RCAs	640:671	When the proper concentration of RCAs was incorporated into the composites, improved water vapor permeability (WVP), oxygen permeability (OP), mechanical, UV-blocking, and antioxidant properties were observed.
33636274	2	59	theme	significant	386:396	arg1	red-pink-blue-green					416:434	red-pink-blue-green	416:434	red-pink-blue-green	416:434	Red cabbage anthocyanins (RCAs) solution illustrated significant color variations (red-pink-blue-green) in different pH environments.
33636274	2	59	theme	significant	386:396	arg1	variations					404:413	significant color variations	386:413	significant color variations (red-pink-blue-green) in different pH environments	386:464	Red cabbage anthocyanins (RCAs) solution illustrated significant color variations (red-pink-blue-green) in different pH environments.
33636274	0	60	theme	cabbage	59:65	arg1	anthocyanins					67:78	red cabbage anthocyanins	55:78	red cabbage anthocyanins	55:78	Fabrication of halochromic smart films by immobilizing red cabbage anthocyanins into chitosan/oxidized-chitin nanocrystals composites for real-time hairtail and shrimp freshness monitoring.
33636274	5	61	theme	naked	1022:1026	arg1	eyes					1028:1031	naked eyes	1022:1031	naked eyes	1022:1031	Moreover, the smart films exhibited distinguishable changes of color to ammonia vapor and acidic/alkaline environment within short time intervals, which were easy to discern by naked eyes.
33636274	4	62	theme	improved	711:718	arg1	WVP					746:748	WVP	746:748	WVP	746:748	When the proper concentration of RCAs was incorporated into the composites, improved water vapor permeability (WVP), oxygen permeability (OP), mechanical, UV-blocking, and antioxidant properties were observed.
33636274	4	62	theme	improved	711:718	arg1	permeability					732:743	improved water vapor permeability	711:743	improved water vapor permeability (WVP)	711:749	When the proper concentration of RCAs was incorporated into the composites, improved water vapor permeability (WVP), oxygen permeability (OP), mechanical, UV-blocking, and antioxidant properties were observed.
33636274	4	62	theme	improved	711:718	arg1	composites					699:708	the composites	695:708	the composites	695:708	When the proper concentration of RCAs was incorporated into the composites, improved water vapor permeability (WVP), oxygen permeability (OP), mechanical, UV-blocking, and antioxidant properties were observed.
33636274	4	63	theme	proper	644:649	arg1	concentration					651:663	the proper concentration	640:663	the proper concentration of RCAs	640:671	When the proper concentration of RCAs was incorporated into the composites, improved water vapor permeability (WVP), oxygen permeability (OP), mechanical, UV-blocking, and antioxidant properties were observed.
34175337	0	0	theme	Structural	90:99	arg1	characteristics					101:115	Structural characteristics	90:115	Structural characteristics	90:115	Exopolysaccharides produced by Pediococcus acidilactici MT41-11 isolated from camel milk: Structural characteristics and bioactive properties.
34175337	6	1	used	used	1135:1138	arg2	MT41-11					1120:1126	P. acidilactici MT41-11	1104:1126	P. acidilactici MT41-11	1104:1126	Overall, the analysis of all data showed EPS from P. acidilactici MT41-11 can be used as anti-oxidant, anti-biofilm agent, and also as a potential candidate prebiotic for health food or medicine industry.
34175337	6	1	used	used	1135:1138	arg2	anti-oxidant					1143:1154	anti-oxidant	1143:1154	anti-oxidant	1143:1154	Overall, the analysis of all data showed EPS from P. acidilactici MT41-11 can be used as anti-oxidant, anti-biofilm agent, and also as a potential candidate prebiotic for health food or medicine industry.
34175337	1	2	theme	chemical	162:169	arg1	structure					171:179	chemical structure	162:179	chemical structure	162:179	In this study, the chemical structure and bioactive properties of the EPS of Pediococcus acidilactici MT41-11 isolated from camel milk were investigated.
34175337	4	3	theme	multiple	884:891	arg1	properties					903:912	multiple bioactive properties	884:912	multiple bioactive properties	884:912	Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity.
34175337	4	3	theme	multiple	884:891	arg1	activity					998:1005	a high DPPH free radical scavenging activity	962:1005	a high DPPH free radical scavenging activity	962:1005	Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity.
34175337	4	3	theme	multiple	884:891	arg1	growth					951:956	stimulating Lactobacillus growth	925:956	stimulating Lactobacillus growth	925:956	Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity.
34175337	1	4	theme	camel	267:271	arg1	milk					273:276	camel milk	267:276	camel milk	267:276	In this study, the chemical structure and bioactive properties of the EPS of Pediococcus acidilactici MT41-11 isolated from camel milk were investigated.
34175337	6	5	theme	acidilactici	1107:1118	arg1	anti-oxidant					1143:1154	anti-oxidant	1143:1154	anti-oxidant	1143:1154	Overall, the analysis of all data showed EPS from P. acidilactici MT41-11 can be used as anti-oxidant, anti-biofilm agent, and also as a potential candidate prebiotic for health food or medicine industry.
34175337	6	5	theme	acidilactici	1107:1118	arg1	MT41-11					1120:1126	P. acidilactici MT41-11	1104:1126	P. acidilactici MT41-11	1104:1126	Overall, the analysis of all data showed EPS from P. acidilactici MT41-11 can be used as anti-oxidant, anti-biofilm agent, and also as a potential candidate prebiotic for health food or medicine industry.
34175337	3	6	theme	2D	522:523	arg1	spectra					529:535	2D NMR spectra	522:535	2D NMR spectra	522:535	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	3	7	theme	-α-d-Glcp-	765:774	arg1	β-d-Glcp-					798:806	→2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→	744:827	β-d-Glcp-	798:806	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	4	8	dep	in	842:843	arg1	vitro					845:849	vitro	845:849	vitro	845:849	Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity.
34175337	2	9	theme	polysaccharide	301:314	arg1	fractions					316:324	Two polysaccharide fractions	297:324	Two polysaccharide fractions (EPS-1, EPS-2) with molecular weights about 69.0 kDa	297:377	Two polysaccharide fractions (EPS-1, EPS-2) with molecular weights about 69.0 kDa were obtained, which were purified using DEAE-Sepharose and Sephadex G-100 chromatography.
34175337	2	10	dep	fractions	316:324	arg1	EPS-1					327:331	EPS-1	327:331	EPS-1	327:331	Two polysaccharide fractions (EPS-1, EPS-2) with molecular weights about 69.0 kDa were obtained, which were purified using DEAE-Sepharose and Sephadex G-100 chromatography.
34175337	2	10	dep	fractions	316:324	arg1	EPS-2					334:338	EPS-2	334:338	EPS-2	334:338	Two polysaccharide fractions (EPS-1, EPS-2) with molecular weights about 69.0 kDa were obtained, which were purified using DEAE-Sepharose and Sephadex G-100 chromatography.
34175337	3	11	theme	-α-d-Glcp-	783:792	arg1	β-d-Glcp-					798:806	→2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→	744:827	β-d-Glcp-	798:806	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	4	12	theme	scavenging	987:996	arg1	activity					998:1005	a high DPPH free radical scavenging activity	962:1005	a high DPPH free radical scavenging activity	962:1005	Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity.
34175337	4	13	theme	radical	979:985	arg1	activity					998:1005	a high DPPH free radical scavenging activity	962:1005	a high DPPH free radical scavenging activity	962:1005	Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity.
34175337	4	14	theme	free	974:977	arg1	radical					979:985	a high DPPH free radical	962:985	a high DPPH free radical scavenging activity	962:1005	Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity.
34175337	6	15	theme	potential	1191:1199	arg1	candidate					1201:1209	a potential candidate	1189:1209	a potential candidate prebiotic for health food or medicine industry	1189:1256	Overall, the analysis of all data showed EPS from P. acidilactici MT41-11 can be used as anti-oxidant, anti-biofilm agent, and also as a potential candidate prebiotic for health food or medicine industry.
34175337	0	16	theme	bioactive	121:129	arg1	properties					131:140	bioactive properties	121:140	bioactive properties	121:140	Exopolysaccharides produced by Pediococcus acidilactici MT41-11 isolated from camel milk: Structural characteristics and bioactive properties.
34175337	3	17	theme	-α-l-Fucp-	747:756	arg1	β-d-Glcp-					798:806	→2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→	744:827	β-d-Glcp-	798:806	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	1	18	theme	bioactive	185:193	arg1	properties					195:204	bioactive properties	185:204	bioactive properties	185:204	In this study, the chemical structure and bioactive properties of the EPS of Pediococcus acidilactici MT41-11 isolated from camel milk were investigated.
34175337	6	19	theme	data	1083:1086	arg1	analysis					1067:1074	the analysis	1063:1074	the analysis of all data	1063:1086	Overall, the analysis of all data showed EPS from P. acidilactici MT41-11 can be used as anti-oxidant, anti-biofilm agent, and also as a potential candidate prebiotic for health food or medicine industry.
34175337	4	20	contain	possessed	874:882	arg2	growth					951:956	stimulating Lactobacillus growth	925:956	stimulating Lactobacillus growth	925:956	Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity.
34175337	4	20	contain	possessed	874:882	arg2	activity					998:1005	a high DPPH free radical scavenging activity	962:1005	a high DPPH free radical scavenging activity	962:1005	Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity.
34175337	4	20	contain	possessed	874:882	arg1	EPS					870:872	EPS	870:872	EPS	870:872	Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity.
34175337	4	20	contain	possessed	874:882	arg2	properties					903:912	multiple bioactive properties	884:912	multiple bioactive properties	884:912	Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity.
34175337	3	21	dep	α-d-Glcp-	816:824	arg1	1→					826:827	1→	826:827	1→	826:827	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	3	22	contain	containing	639:648	arg2	α-d-Manp-					650:658	α-d-Manp-	650:658	α-d-Manp-	650:658	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	3	22	contain	containing	639:648	arg1	branches					630:637	branches	630:637	branches containing α-d-Manp-	630:658	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	0	23	attach	isolated	64:71	arg2	MT41-11					56:62	Pediococcus acidilactici MT41-11	31:62	Pediococcus acidilactici MT41-11 isolated from camel milk	31:87	Exopolysaccharides produced by Pediococcus acidilactici MT41-11 isolated from camel milk: Structural characteristics and bioactive properties.
34175337	0	23	attach	isolated	64:71	arg1	milk					84:87	camel milk	78:87	camel milk	78:87	Exopolysaccharides produced by Pediococcus acidilactici MT41-11 isolated from camel milk: Structural characteristics and bioactive properties.
34175337	1	24	dep	structure	171:179	arg1	the					158:160	the	158:160	the	158:160	In this study, the chemical structure and bioactive properties of the EPS of Pediococcus acidilactici MT41-11 isolated from camel milk were investigated.
34175337	0	25	theme	Pediococcus	31:41	arg1	MT41-11					56:62	Pediococcus acidilactici MT41-11	31:62	Pediococcus acidilactici MT41-11 isolated from camel milk	31:87	Exopolysaccharides produced by Pediococcus acidilactici MT41-11 isolated from camel milk: Structural characteristics and bioactive properties.
34175337	3	26	contain	had	559:561	arg1	EPS-1					553:557	EPS-1	553:557	EPS-1	553:557	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	3	26	contain	had	559:561	arg2	backbone					565:572	a backbone	563:572	a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-	563:658	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	2	27	theme	Sephadex	439:446	arg1	chromatography					454:467	DEAE-Sepharose and Sephadex G-100 chromatography	420:467	chromatography	454:467	Two polysaccharide fractions (EPS-1, EPS-2) with molecular weights about 69.0 kDa were obtained, which were purified using DEAE-Sepharose and Sephadex G-100 chromatography.
34175337	2	28	theme	G-100	448:452	arg1	chromatography					454:467	DEAE-Sepharose and Sephadex G-100 chromatography	420:467	chromatography	454:467	Two polysaccharide fractions (EPS-1, EPS-2) with molecular weights about 69.0 kDa were obtained, which were purified using DEAE-Sepharose and Sephadex G-100 chromatography.
34175337	5	29	theme	good	1023:1026	arg1	ability					1028:1034	a good ability	1021:1034	a good ability to anti-biofilms	1021:1051	Also, it has a good ability to anti-biofilms.
34175337	6	30	theme	anti-biofilm	1157:1168	arg1	anti-oxidant					1143:1154	anti-oxidant	1143:1154	anti-oxidant	1143:1154	Overall, the analysis of all data showed EPS from P. acidilactici MT41-11 can be used as anti-oxidant, anti-biofilm agent, and also as a potential candidate prebiotic for health food or medicine industry.
34175337	6	30	theme	anti-biofilm	1157:1168	arg1	agent					1170:1174	anti-biofilm agent	1157:1174	anti-biofilm agent	1157:1174	Overall, the analysis of all data showed EPS from P. acidilactici MT41-11 can be used as anti-oxidant, anti-biofilm agent, and also as a potential candidate prebiotic for health food or medicine industry.
34175337	3	31	theme	1→	794:795	arg1	β-d-Glcp-					798:806	→2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→	744:827	β-d-Glcp-	798:806	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	1	32	theme	EPS	213:215	arg1	properties					195:204	bioactive properties	185:204	bioactive properties	185:204	In this study, the chemical structure and bioactive properties of the EPS of Pediococcus acidilactici MT41-11 isolated from camel milk were investigated.
34175337	1	32	theme	EPS	213:215	arg1	structure					171:179	chemical structure	162:179	chemical structure	162:179	In this study, the chemical structure and bioactive properties of the EPS of Pediococcus acidilactici MT41-11 isolated from camel milk were investigated.
34175337	4	33	theme	in	842:843	arg1	assays					851:856	in vitro assays	842:856	in vitro assays	842:856	Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity.
34175337	4	34	theme	high	964:967	arg1	radical					979:985	a high DPPH free radical	962:985	a high DPPH free radical scavenging activity	962:1005	Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity.
34175337	2	35	theme	DEAE-Sepharose	420:433	arg1	chromatography					454:467	DEAE-Sepharose and Sephadex G-100 chromatography	420:467	chromatography	454:467	Two polysaccharide fractions (EPS-1, EPS-2) with molecular weights about 69.0 kDa were obtained, which were purified using DEAE-Sepharose and Sephadex G-100 chromatography.
34175337	0	36	theme	acidilactici	43:54	arg1	MT41-11					56:62	Pediococcus acidilactici MT41-11	31:62	Pediococcus acidilactici MT41-11 isolated from camel milk	31:87	Exopolysaccharides produced by Pediococcus acidilactici MT41-11 isolated from camel milk: Structural characteristics and bioactive properties.
34175337	5	37	contain	has	1017:1019	arg2	ability					1028:1034	a good ability	1021:1034	a good ability to anti-biofilms	1021:1051	Also, it has a good ability to anti-biofilms.
34175337	5	37	contain	has	1017:1019	arg1	it					1014:1015	it	1014:1015	it	1014:1015	Also, it has a good ability to anti-biofilms.
34175337	3	38	theme	-α-d-Manp-	589:598	arg1	1→					618:619	→2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→	586:619	1→	618:619	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	1	39	theme	Pediococcus	220:230	arg1	MT41-11					245:251	Pediococcus acidilactici MT41-11	220:251	Pediococcus acidilactici MT41-11 isolated from camel milk	220:276	In this study, the chemical structure and bioactive properties of the EPS of Pediococcus acidilactici MT41-11 isolated from camel milk were investigated.
34175337	6	40	theme	health	1225:1230	arg1	food					1232:1235	health food	1225:1235	health food	1225:1235	Overall, the analysis of all data showed EPS from P. acidilactici MT41-11 can be used as anti-oxidant, anti-biofilm agent, and also as a potential candidate prebiotic for health food or medicine industry.
34175337	3	41	theme	-α-d-Manp-	607:616	arg1	1→					618:619	→2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→	586:619	1→	618:619	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	4	42	theme	DPPH	969:972	arg1	radical					979:985	a high DPPH free radical	962:985	a high DPPH free radical scavenging activity	962:1005	Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity.
34175337	3	43	contain	had	670:672	arg2	backbone					676:683	a backbone	674:683	a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→	674:827	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	3	43	contain	had	670:672	arg1	EPS-2					664:668	EPS-2	664:668	EPS-2	664:668	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	6	44	dep	food	1232:1235	arg1	industry					1249:1256	industry	1249:1256	industry	1249:1256	Overall, the analysis of all data showed EPS from P. acidilactici MT41-11 can be used as anti-oxidant, anti-biofilm agent, and also as a potential candidate prebiotic for health food or medicine industry.
34175337	3	45	theme	-β-d-Glcp-	700:709	arg1	1→					711:712	→6)-β-d-Glcp-(1→	697:712	→6)-β-d-Glcp-(1→	697:712	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	1	46	attach	isolated	253:260	arg1	milk					273:276	camel milk	267:276	camel milk	267:276	In this study, the chemical structure and bioactive properties of the EPS of Pediococcus acidilactici MT41-11 isolated from camel milk were investigated.
34175337	1	46	attach	isolated	253:260	arg2	MT41-11					245:251	Pediococcus acidilactici MT41-11	220:251	Pediococcus acidilactici MT41-11 isolated from camel milk	220:276	In this study, the chemical structure and bioactive properties of the EPS of Pediococcus acidilactici MT41-11 isolated from camel milk were investigated.
34175337	2	47	theme	molecular	346:354	arg1	weights					356:362	molecular weights	346:362	molecular weights about 69.0 kDa	346:377	Two polysaccharide fractions (EPS-1, EPS-2) with molecular weights about 69.0 kDa were obtained, which were purified using DEAE-Sepharose and Sephadex G-100 chromatography.
34175337	4	48	theme	stimulating	925:935	arg1	growth					951:956	stimulating Lactobacillus growth	925:956	stimulating Lactobacillus growth	925:956	Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity.
34175337	1	49	theme	acidilactici	232:243	arg1	MT41-11					245:251	Pediococcus acidilactici MT41-11	220:251	Pediococcus acidilactici MT41-11 isolated from camel milk	220:276	In this study, the chemical structure and bioactive properties of the EPS of Pediococcus acidilactici MT41-11 isolated from camel milk were investigated.
34175337	3	50	theme	1→	758:759	arg1	β-d-Glcp-					798:806	→2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→	744:827	β-d-Glcp-	798:806	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	1	51	theme	MT41-11	245:251	arg1	EPS					213:215	the EPS	209:215	the EPS of Pediococcus acidilactici MT41-11 isolated from camel milk	209:276	In this study, the chemical structure and bioactive properties of the EPS of Pediococcus acidilactici MT41-11 isolated from camel milk were investigated.
34175337	6	52	theme	prebiotic	1211:1219	arg1	candidate					1201:1209	a potential candidate	1189:1209	a potential candidate prebiotic for health food or medicine industry	1189:1256	Overall, the analysis of all data showed EPS from P. acidilactici MT41-11 can be used as anti-oxidant, anti-biofilm agent, and also as a potential candidate prebiotic for health food or medicine industry.
34175337	3	53	theme	monosaccharide	479:492	arg1	composition					494:504	monosaccharide composition	479:504	monosaccharide composition	479:504	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	3	54	contain	containing	733:742	arg1	branches					724:731	branches	724:731	branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→	724:827	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	3	54	contain	containing	733:742	arg2	→2					780:781	→2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→	744:827	→2	780:781	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	3	54	contain	containing	733:742	arg2	β-d-Glcp-					798:806	→2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→	744:827	β-d-Glcp-	798:806	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	3	54	contain	containing	733:742	arg2	α-d-Glcp-					816:824	α-d-Glcp-	816:824	α-d-Glcp-(1→	816:827	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	3	54	contain	containing	733:742	arg2	→3					762:763	→2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→	744:827	→3	762:763	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	4	55	theme	bioactive	893:901	arg1	properties					903:912	multiple bioactive properties	884:912	multiple bioactive properties	884:912	Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity.
34175337	4	55	theme	bioactive	893:901	arg1	activity					998:1005	a high DPPH free radical scavenging activity	962:1005	a high DPPH free radical scavenging activity	962:1005	Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity.
34175337	4	55	theme	bioactive	893:901	arg1	growth					951:956	stimulating Lactobacillus growth	925:956	stimulating Lactobacillus growth	925:956	Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity.
34175337	3	56	theme	NMR	525:527	arg1	spectra					529:535	2D NMR spectra	522:535	2D NMR spectra	522:535	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34175337	4	57	theme	Lactobacillus	937:949	arg1	growth					951:956	stimulating Lactobacillus growth	925:956	stimulating Lactobacillus growth	925:956	Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity.
34175337	2	58	with	fractions	316:324	arg1	weights					356:362	molecular weights	346:362	molecular weights about 69.0 kDa	346:377	Two polysaccharide fractions (EPS-1, EPS-2) with molecular weights about 69.0 kDa were obtained, which were purified using DEAE-Sepharose and Sephadex G-100 chromatography.
34175337	0	59	theme	camel	78:82	arg1	milk					84:87	camel milk	78:87	camel milk	78:87	Exopolysaccharides produced by Pediococcus acidilactici MT41-11 isolated from camel milk: Structural characteristics and bioactive properties.
34175337	6	60	theme	P.	1104:1105	arg1	anti-oxidant					1143:1154	anti-oxidant	1143:1154	anti-oxidant	1143:1154	Overall, the analysis of all data showed EPS from P. acidilactici MT41-11 can be used as anti-oxidant, anti-biofilm agent, and also as a potential candidate prebiotic for health food or medicine industry.
34175337	6	60	theme	P.	1104:1105	arg1	MT41-11					1120:1126	P. acidilactici MT41-11	1104:1126	P. acidilactici MT41-11	1104:1126	Overall, the analysis of all data showed EPS from P. acidilactici MT41-11 can be used as anti-oxidant, anti-biofilm agent, and also as a potential candidate prebiotic for health food or medicine industry.
34175337	3	61	theme	1→	776:777	arg1	β-d-Glcp-					798:806	→2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→	744:827	β-d-Glcp-	798:806	Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→.
34436648	7	0	theme	structural	1369:1378	arg1	components					1380:1389	the known structural components	1359:1389	the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin	1359:1510	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	6	1	from	pulp	1206:1209	arg1	present					1187:1193	present	1187:1193	present	1187:1193	The AXC-MEC is highly capable of degrading starch and non-starch polysaccharides present in cassava pulp into glucose and oligosaccharides, without conventional pretreatment.
34436648	7	2	from	components	1380:1389	arg1	cohesin					1490:1496	cohesin	1490:1496	cohesin	1490:1496	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	7	2	from	components	1380:1389	arg1	cellulosomes					1457:1468	cellulosomes	1457:1468	cellulosomes such as scaffoldin, cohesin, and dockerin	1457:1510	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	7	2	from	components	1380:1389	arg1	scaffoldin					1478:1487	scaffoldin	1478:1487	scaffoldin	1478:1487	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	7	2	from	components	1380:1389	arg1	species					1448:1454	Clostridium species	1436:1454	Clostridium species	1436:1454	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	7	2	from	components	1380:1389	arg1	dockerin					1503:1510	dockerin	1503:1510	dockerin	1503:1510	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	3	3	theme	manihotivorum CT4	624:640	arg1	enzyme					567:572	crude extracellular enzyme	547:572	crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp	547:663	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	1	4	theme	anaerobic	149:157	arg1	species					194:200	Clostridium species	182:200	Clostridium species	182:200	Some anaerobic bacteria, particularly Clostridium species, produce extracellular cellulolytic and xylanolytic enzymes as multienzyme complexes (MECs).
34436648	1	4	theme	anaerobic	149:157	arg1	bacteria					159:166	Some anaerobic bacteria	144:166	Some anaerobic bacteria	144:166	Some anaerobic bacteria, particularly Clostridium species, produce extracellular cellulolytic and xylanolytic enzymes as multienzyme complexes (MECs).
34436648	9	5	theme	•	1804:1804	arg1	AXC-MEC					1819:1825	• Glycoprotein AXC-MEC	1804:1825	• Glycoprotein AXC-MEC	1804:1825	KEY POINTS: • Glycoprotein AXC-MEC was first reported in Clostridium manihotivorum.
34436648	2	6	theme	multienzyme	343:353	arg1	AXC-MEC					364:370	AXC-MEC	364:370	AXC-MEC	364:370	However, an amylolytic/xylanolytic/cellulolytic multienzyme complex (AXC-MEC) from anaerobic bacteria is rarely found.
34436648	2	6	theme	multienzyme	343:353	arg1	complex					355:361	an amylolytic/xylanolytic/cellulolytic multienzyme complex	304:361	an amylolytic/xylanolytic/cellulolytic multienzyme complex (AXC-MEC) from anaerobic bacteria	304:395	However, an amylolytic/xylanolytic/cellulolytic multienzyme complex (AXC-MEC) from anaerobic bacteria is rarely found.
34436648	1	7	theme	cellulolytic	225:236	arg1	enzymes					254:260	extracellular cellulolytic and xylanolytic enzymes	211:260	extracellular cellulolytic and xylanolytic enzymes as multienzyme complexes (MECs)	211:292	Some anaerobic bacteria, particularly Clostridium species, produce extracellular cellulolytic and xylanolytic enzymes as multienzyme complexes (MECs).
34436648	2	8	theme	anaerobic	378:386	arg1	bacteria					388:395	anaerobic bacteria	378:395	anaerobic bacteria	378:395	However, an amylolytic/xylanolytic/cellulolytic multienzyme complex (AXC-MEC) from anaerobic bacteria is rarely found.
34436648	1	9	theme	xylanolytic	242:252	arg1	enzymes					254:260	extracellular cellulolytic and xylanolytic enzymes	211:260	extracellular cellulolytic and xylanolytic enzymes as multienzyme complexes (MECs)	211:292	Some anaerobic bacteria, particularly Clostridium species, produce extracellular cellulolytic and xylanolytic enzymes as multienzyme complexes (MECs).
34436648	9	10	dep	POINTS	1796:1801	arg1	reported					1837:1844	reported	1837:1844	was first reported in Clostridium manihotivorum	1827:1873	KEY POINTS: • Glycoprotein AXC-MEC was first reported in Clostridium manihotivorum.
34436648	2	11	theme	amylolytic/xylanolytic/cellulolytic	307:341	arg1	AXC-MEC					364:370	AXC-MEC	364:370	AXC-MEC	364:370	However, an amylolytic/xylanolytic/cellulolytic multienzyme complex (AXC-MEC) from anaerobic bacteria is rarely found.
34436648	2	11	theme	amylolytic/xylanolytic/cellulolytic	307:341	arg1	complex					355:361	an amylolytic/xylanolytic/cellulolytic multienzyme complex	304:361	an amylolytic/xylanolytic/cellulolytic multienzyme complex (AXC-MEC) from anaerobic bacteria	304:395	However, an amylolytic/xylanolytic/cellulolytic multienzyme complex (AXC-MEC) from anaerobic bacteria is rarely found.
34436648	4	12	theme	native-polyacrylamide	799:819	arg1	native-PAGE					842:852	native-PAGE	842:852	native-PAGE	842:852	The isolated AXC-MEC showed a single band upon native-polyacrylamide gel electrophoresis (native-PAGE).
34436648	4	12	theme	native-polyacrylamide	799:819	arg1	electrophoresis					825:839	native-polyacrylamide gel electrophoresis	799:839	native-polyacrylamide gel electrophoresis (native-PAGE)	799:853	The isolated AXC-MEC showed a single band upon native-polyacrylamide gel electrophoresis (native-PAGE).
34436648	7	13	from	CT4	1549:1551	arg1	AXC-MEC					1529:1535	AXC-MEC	1529:1535	AXC-MEC from strain CT4	1529:1551	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	6	14	theme	starch	1149:1154	arg1	polysaccharides					1171:1185	starch and non-starch polysaccharides	1149:1185	starch and non-starch polysaccharides present in cassava pulp	1149:1209	The AXC-MEC is highly capable of degrading starch and non-starch polysaccharides present in cassava pulp into glucose and oligosaccharides, without conventional pretreatment.
34436648	7	15	theme	known	1363:1367	arg1	components					1380:1389	the known structural components	1359:1389	the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin	1359:1510	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	0	16	theme	amylolytic/xylanolytic/cellulolytic	8:42	arg1	complex					56:62	A novel amylolytic/xylanolytic/cellulolytic multienzyme complex	0:62	A novel amylolytic/xylanolytic/cellulolytic multienzyme complex from Clostridium manihotivorum that hydrolyzes polysaccharides in cassava pulp.	0:142	A novel amylolytic/xylanolytic/cellulolytic multienzyme complex from Clostridium manihotivorum that hydrolyzes polysaccharides in cassava pulp.
34436648	5	17	theme	amylolytic	1018:1027	arg1	activity					1036:1043	amylolytic enzyme activity	1018:1043	amylolytic enzyme activity	1018:1043	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed at least eight protein bands of the multienzyme complex which predominantly exhibited amylolytic enzyme activity, followed by xylanolytic and cellulolytic enzyme activities.
34436648	2	18	from	bacteria	388:395	arg1	AXC-MEC					364:370	AXC-MEC	364:370	AXC-MEC	364:370	However, an amylolytic/xylanolytic/cellulolytic multienzyme complex (AXC-MEC) from anaerobic bacteria is rarely found.
34436648	2	18	from	bacteria	388:395	arg1	complex					355:361	an amylolytic/xylanolytic/cellulolytic multienzyme complex	304:361	an amylolytic/xylanolytic/cellulolytic multienzyme complex (AXC-MEC) from anaerobic bacteria	304:395	However, an amylolytic/xylanolytic/cellulolytic multienzyme complex (AXC-MEC) from anaerobic bacteria is rarely found.
34436648	11	19	from	pulp	2022:2025	arg1	products					2000:2007	hydrolysis products	1989:2007	hydrolysis products from cassava pulp by AXC-MEC	1989:2036	• Glucose and oligosaccharides were hydrolysis products from cassava pulp by AXC-MEC.
34436648	0	20	theme	cassava	130:136	arg1	pulp					138:141	cassava pulp	130:141	cassava pulp	130:141	A novel amylolytic/xylanolytic/cellulolytic multienzyme complex from Clostridium manihotivorum that hydrolyzes polysaccharides in cassava pulp.
34436648	11	21	dep	•	1953:1953	arg1	Glucose					1955:1961	Glucose	1955:1961	Glucose	1955:1961	• Glucose and oligosaccharides were hydrolysis products from cassava pulp by AXC-MEC.
34436648	11	21	dep	•	1953:1953	arg1	oligosaccharides					1967:1982	oligosaccharides	1967:1982	oligosaccharides	1967:1982	• Glucose and oligosaccharides were hydrolysis products from cassava pulp by AXC-MEC.
34436648	3	22	theme	xylanolytic	490:500	arg1	enzymes					520:526	amylolytic, xylanolytic, and cellulolytic enzymes	478:526	amylolytic, xylanolytic, and cellulolytic enzymes	478:526	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	5	23	theme	sulfate-polyacrylamide	871:892	arg1	electrophoresis					898:912	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis	856:912	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)	856:923	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed at least eight protein bands of the multienzyme complex which predominantly exhibited amylolytic enzyme activity, followed by xylanolytic and cellulolytic enzyme activities.
34436648	5	23	theme	sulfate-polyacrylamide	871:892	arg1	SDS-PAGE					915:922	SDS-PAGE	915:922	SDS-PAGE	915:922	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed at least eight protein bands of the multienzyme complex which predominantly exhibited amylolytic enzyme activity, followed by xylanolytic and cellulolytic enzyme activities.
34436648	3	24	theme	amylolytic	478:487	arg1	enzymes					520:526	amylolytic, xylanolytic, and cellulolytic enzymes	478:526	amylolytic, xylanolytic, and cellulolytic enzymes	478:526	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	7	25	theme	CT4	1330:1332	arg1	analysis					1301:1308	the genomic analysis	1289:1308	the genomic analysis of C. manihotivorum CT4	1289:1332	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	5	26	theme	xylanolytic	1058:1068	arg1	activities					1094:1103	xylanolytic and cellulolytic enzyme activities	1058:1103	xylanolytic and cellulolytic enzyme activities	1058:1103	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed at least eight protein bands of the multienzyme complex which predominantly exhibited amylolytic enzyme activity, followed by xylanolytic and cellulolytic enzyme activities.
34436648	6	27	theme	cassava	1198:1204	arg1	pulp					1206:1209	cassava pulp	1198:1209	cassava pulp	1198:1209	The AXC-MEC is highly capable of degrading starch and non-starch polysaccharides present in cassava pulp into glucose and oligosaccharides, without conventional pretreatment.
34436648	3	28	theme	enzymes	520:526	arg1	subunits					466:473	subunits	466:473	subunits of amylolytic, xylanolytic, and cellulolytic enzymes	466:526	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	7	29	theme	C.	1313:1314	arg1	CT4					1330:1332	C. manihotivorum CT4	1313:1332	C. manihotivorum CT4	1313:1332	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	5	30	theme	cellulolytic	1074:1085	arg1	activities					1094:1103	xylanolytic and cellulolytic enzyme activities	1058:1103	xylanolytic and cellulolytic enzyme activities	1058:1103	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed at least eight protein bands of the multienzyme complex which predominantly exhibited amylolytic enzyme activity, followed by xylanolytic and cellulolytic enzyme activities.
34436648	6	31	theme	present	1187:1193	arg1	polysaccharides					1171:1185	starch and non-starch polysaccharides	1149:1185	starch and non-starch polysaccharides present in cassava pulp	1149:1209	The AXC-MEC is highly capable of degrading starch and non-starch polysaccharides present in cassava pulp into glucose and oligosaccharides, without conventional pretreatment.
34436648	3	32	theme	gel	721:723	arg1	chromatography					736:749	a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography	672:749	chromatography	736:749	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	5	33	theme	gel	894:896	arg1	electrophoresis					898:912	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis	856:912	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)	856:923	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed at least eight protein bands of the multienzyme complex which predominantly exhibited amylolytic enzyme activity, followed by xylanolytic and cellulolytic enzyme activities.
34436648	5	33	theme	gel	894:896	arg1	SDS-PAGE					915:922	SDS-PAGE	915:922	SDS-PAGE	915:922	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed at least eight protein bands of the multienzyme complex which predominantly exhibited amylolytic enzyme activity, followed by xylanolytic and cellulolytic enzyme activities.
34436648	6	34	theme	non-starch	1160:1169	arg1	polysaccharides					1171:1185	starch and non-starch polysaccharides	1149:1185	starch and non-starch polysaccharides present in cassava pulp	1149:1209	The AXC-MEC is highly capable of degrading starch and non-starch polysaccharides present in cassava pulp into glucose and oligosaccharides, without conventional pretreatment.
34436648	8	35	theme	cassava	1712:1718	arg1	pulp					1720:1723	cassava pulp	1712:1723	cassava pulp	1712:1723	These results suggest that AXC-MEC from C. manihotivorum CT4 is a new MEC capable of hydrolyzing cassava pulp into value-added products, which will benefit the starch industry.
34436648	3	36	theme	Sephacryl	705:713	arg1	chromatography					736:749	a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography	672:749	chromatography	736:749	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	8	37	from	manihotivorum CT4	1658:1674	arg1	MEC					1685:1687	a new MEC	1679:1687	a new MEC capable of hydrolyzing cassava pulp into value-added products, which will benefit the starch industry	1679:1789	These results suggest that AXC-MEC from C. manihotivorum CT4 is a new MEC capable of hydrolyzing cassava pulp into value-added products, which will benefit the starch industry.
34436648	8	37	from	manihotivorum CT4	1658:1674	arg1	AXC-MEC					1642:1648	AXC-MEC	1642:1648	AXC-MEC from C. manihotivorum CT4	1642:1674	These results suggest that AXC-MEC from C. manihotivorum CT4 is a new MEC capable of hydrolyzing cassava pulp into value-added products, which will benefit the starch industry.
34436648	7	38	from	Base	1281:1284	arg1	analysis					1301:1308	the genomic analysis	1289:1308	the genomic analysis of C. manihotivorum CT4	1289:1332	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	11	39	theme	cassava	2014:2020	arg1	pulp					2022:2025	cassava pulp	2014:2025	cassava pulp by AXC-MEC	2014:2036	• Glucose and oligosaccharides were hydrolysis products from cassava pulp by AXC-MEC.
34436648	3	40	theme	anaerobic	592:600	arg1	manihotivorum CT4					624:640	the mesophilic anaerobic bacterium Clostridium manihotivorum CT4	577:640	the mesophilic anaerobic bacterium Clostridium manihotivorum CT4	577:640	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	7	41	theme	multienzyme	1409:1419	arg1	complexes					1421:1429	the well-known multienzyme complexes	1394:1429	the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin	1394:1510	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	3	42	attach	isolated	533:540	arg1	enzyme					567:572	crude extracellular enzyme	547:572	crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp	547:663	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	3	42	attach	isolated	533:540	arg2	AXC-MEC					445:451	the glycoprotein AXC-MEC	428:451	the glycoprotein AXC-MEC	428:451	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	3	43	theme	extracellular	553:565	arg1	enzyme					567:572	crude extracellular enzyme	547:572	crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp	547:663	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	9	44	theme	KEY	1792:1794	arg1	POINTS					1796:1801	KEY POINTS	1792:1801	KEY POINTS: • Glycoprotein AXC-MEC was first reported in Clostridium manihotivorum.	1792:1874	KEY POINTS: • Glycoprotein AXC-MEC was first reported in Clostridium manihotivorum.
34436648	7	45	theme	components	1380:1389	arg1	evidence					1347:1354	no evidence	1344:1354	no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin	1344:1510	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	3	46	theme	bacterium Clostridium	602:622	arg1	manihotivorum CT4					624:640	the mesophilic anaerobic bacterium Clostridium manihotivorum CT4	577:640	the mesophilic anaerobic bacterium Clostridium manihotivorum CT4	577:640	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	8	47	theme	starch	1775:1780	arg1	industry					1782:1789	the starch industry	1771:1789	the starch industry	1771:1789	These results suggest that AXC-MEC from C. manihotivorum CT4 is a new MEC capable of hydrolyzing cassava pulp into value-added products, which will benefit the starch industry.
34436648	3	48	theme	mesophilic	581:590	arg1	manihotivorum CT4					624:640	the mesophilic anaerobic bacterium Clostridium manihotivorum CT4	577:640	the mesophilic anaerobic bacterium Clostridium manihotivorum CT4	577:640	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	1	49	theme	extracellular	211:223	arg1	enzymes					254:260	extracellular cellulolytic and xylanolytic enzymes	211:260	extracellular cellulolytic and xylanolytic enzymes as multienzyme complexes (MECs)	211:292	Some anaerobic bacteria, particularly Clostridium species, produce extracellular cellulolytic and xylanolytic enzymes as multienzyme complexes (MECs).
34436648	7	50	theme	assembly	1583:1590	arg1	manner					1573:1578	a different manner	1561:1578	a different manner of assembly from the cellulosomes	1561:1612	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	9	51	theme	Glycoprotein	1806:1817	arg1	AXC-MEC					1819:1825	• Glycoprotein AXC-MEC	1804:1825	• Glycoprotein AXC-MEC	1804:1825	KEY POINTS: • Glycoprotein AXC-MEC was first reported in Clostridium manihotivorum.
34436648	7	52	theme	different	1563:1571	arg1	manner					1573:1578	a different manner	1561:1578	a different manner of assembly from the cellulosomes	1561:1612	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	5	53	theme	multienzyme	968:978	arg1	complex					980:986	the multienzyme complex	964:986	the multienzyme complex	964:986	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed at least eight protein bands of the multienzyme complex which predominantly exhibited amylolytic enzyme activity, followed by xylanolytic and cellulolytic enzyme activities.
34436648	6	54	from	present	1187:1193	arg1	pulp					1206:1209	cassava pulp	1198:1209	cassava pulp	1198:1209	The AXC-MEC is highly capable of degrading starch and non-starch polysaccharides present in cassava pulp into glucose and oligosaccharides, without conventional pretreatment.
34436648	3	55	theme	cassava	652:658	arg1	pulp					660:663	cassava pulp	652:663	cassava pulp	652:663	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	7	56	theme	strain	1542:1547	arg1	CT4					1549:1551	strain CT4	1542:1551	strain CT4	1542:1551	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	5	57	theme	complex	980:986	arg1	bands					955:959	at least eight protein bands	932:959	at least eight protein bands of the multienzyme complex which predominantly exhibited amylolytic enzyme activity, followed by xylanolytic and cellulolytic enzyme activities	932:1103	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed at least eight protein bands of the multienzyme complex which predominantly exhibited amylolytic enzyme activity, followed by xylanolytic and cellulolytic enzyme activities.
34436648	5	58	theme	protein	947:953	arg1	bands					955:959	at least eight protein bands	932:959	at least eight protein bands of the multienzyme complex which predominantly exhibited amylolytic enzyme activity, followed by xylanolytic and cellulolytic enzyme activities	932:1103	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed at least eight protein bands of the multienzyme complex which predominantly exhibited amylolytic enzyme activity, followed by xylanolytic and cellulolytic enzyme activities.
34436648	9	59	theme	Clostridium	1849:1859	arg1	manihotivorum					1861:1873	Clostridium manihotivorum	1849:1873	Clostridium manihotivorum	1849:1873	KEY POINTS: • Glycoprotein AXC-MEC was first reported in Clostridium manihotivorum.
34436648	5	60	theme	enzyme	1029:1034	arg1	activity					1036:1043	amylolytic enzyme activity	1018:1043	amylolytic enzyme activity	1018:1043	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed at least eight protein bands of the multienzyme complex which predominantly exhibited amylolytic enzyme activity, followed by xylanolytic and cellulolytic enzyme activities.
34436648	4	61	theme	single	782:787	arg1	band					789:792	a single band	780:792	a single band upon native-polyacrylamide gel electrophoresis (native-PAGE)	780:853	The isolated AXC-MEC showed a single band upon native-polyacrylamide gel electrophoresis (native-PAGE).
34436648	3	62	theme	pulp	689:692	arg1	column					694:699	a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography	672:749	column	694:699	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	3	63	theme	milled	674:679	arg1	column					694:699	a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography	672:749	column	694:699	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	1	64	theme	Clostridium	182:192	arg1	species					194:200	Clostridium species	182:200	Clostridium species	182:200	Some anaerobic bacteria, particularly Clostridium species, produce extracellular cellulolytic and xylanolytic enzymes as multienzyme complexes (MECs).
34436648	1	64	theme	Clostridium	182:192	arg1	bacteria					159:166	Some anaerobic bacteria	144:166	Some anaerobic bacteria	144:166	Some anaerobic bacteria, particularly Clostridium species, produce extracellular cellulolytic and xylanolytic enzymes as multienzyme complexes (MECs).
34436648	7	65	from	cellulosomes	1601:1612	arg1	manner					1573:1578	a different manner	1561:1578	a different manner of assembly from the cellulosomes	1561:1612	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	4	66	theme	isolated	756:763	arg1	AXC-MEC					765:771	The isolated AXC-MEC	752:771	The isolated AXC-MEC	752:771	The isolated AXC-MEC showed a single band upon native-polyacrylamide gel electrophoresis (native-PAGE).
34436648	3	67	theme	cassava	681:687	arg1	column					694:699	a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography	672:749	column	694:699	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	8	68	theme	C.	1655:1656	arg1	manihotivorum CT4					1658:1674	C. manihotivorum CT4	1655:1674	C. manihotivorum CT4	1655:1674	These results suggest that AXC-MEC from C. manihotivorum CT4 is a new MEC capable of hydrolyzing cassava pulp into value-added products, which will benefit the starch industry.
34436648	5	69	theme	enzyme	1087:1092	arg1	activities					1094:1103	xylanolytic and cellulolytic enzyme activities	1058:1103	xylanolytic and cellulolytic enzyme activities	1058:1103	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed at least eight protein bands of the multienzyme complex which predominantly exhibited amylolytic enzyme activity, followed by xylanolytic and cellulolytic enzyme activities.
34436648	0	70	from	manihotivorum	81:93	arg1	complex					56:62	A novel amylolytic/xylanolytic/cellulolytic multienzyme complex	0:62	A novel amylolytic/xylanolytic/cellulolytic multienzyme complex from Clostridium manihotivorum that hydrolyzes polysaccharides in cassava pulp.	0:142	A novel amylolytic/xylanolytic/cellulolytic multienzyme complex from Clostridium manihotivorum that hydrolyzes polysaccharides in cassava pulp.
34436648	6	71	theme	conventional	1254:1265	arg1	pretreatment					1267:1278	conventional pretreatment	1254:1278	conventional pretreatment	1254:1278	The AXC-MEC is highly capable of degrading starch and non-starch polysaccharides present in cassava pulp into glucose and oligosaccharides, without conventional pretreatment.
34436648	8	72	theme	new	1681:1683	arg1	MEC					1685:1687	a new MEC	1679:1687	a new MEC capable of hydrolyzing cassava pulp into value-added products, which will benefit the starch industry	1679:1789	These results suggest that AXC-MEC from C. manihotivorum CT4 is a new MEC capable of hydrolyzing cassava pulp into value-added products, which will benefit the starch industry.
34436648	8	72	theme	new	1681:1683	arg1	AXC-MEC					1642:1648	AXC-MEC	1642:1648	AXC-MEC from C. manihotivorum CT4	1642:1674	These results suggest that AXC-MEC from C. manihotivorum CT4 is a new MEC capable of hydrolyzing cassava pulp into value-added products, which will benefit the starch industry.
34436648	0	73	theme	novel	2:6	arg1	complex					56:62	A novel amylolytic/xylanolytic/cellulolytic multienzyme complex	0:62	A novel amylolytic/xylanolytic/cellulolytic multienzyme complex from Clostridium manihotivorum that hydrolyzes polysaccharides in cassava pulp.	0:142	A novel amylolytic/xylanolytic/cellulolytic multienzyme complex from Clostridium manihotivorum that hydrolyzes polysaccharides in cassava pulp.
34436648	3	74	gly	glycoprotein	432:443	arg1	glycoprotein					432:443	the glycoprotein AXC-MEC	428:451	the glycoprotein AXC-MEC	428:451	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	8	75	theme	capable	1689:1695	arg1	MEC					1685:1687	a new MEC	1679:1687	a new MEC capable of hydrolyzing cassava pulp into value-added products, which will benefit the starch industry	1679:1789	These results suggest that AXC-MEC from C. manihotivorum CT4 is a new MEC capable of hydrolyzing cassava pulp into value-added products, which will benefit the starch industry.
34436648	8	75	theme	capable	1689:1695	arg1	AXC-MEC					1642:1648	AXC-MEC	1642:1648	AXC-MEC from C. manihotivorum CT4	1642:1674	These results suggest that AXC-MEC from C. manihotivorum CT4 is a new MEC capable of hydrolyzing cassava pulp into value-added products, which will benefit the starch industry.
34436648	4	76	theme	gel	821:823	arg1	native-PAGE					842:852	native-PAGE	842:852	native-PAGE	842:852	The isolated AXC-MEC showed a single band upon native-polyacrylamide gel electrophoresis (native-PAGE).
34436648	4	76	theme	gel	821:823	arg1	electrophoresis					825:839	native-polyacrylamide gel electrophoresis	799:839	native-polyacrylamide gel electrophoresis (native-PAGE)	799:853	The isolated AXC-MEC showed a single band upon native-polyacrylamide gel electrophoresis (native-PAGE).
34436648	0	77	theme	multienzyme	44:54	arg1	complex					56:62	A novel amylolytic/xylanolytic/cellulolytic multienzyme complex	0:62	A novel amylolytic/xylanolytic/cellulolytic multienzyme complex from Clostridium manihotivorum that hydrolyzes polysaccharides in cassava pulp.	0:142	A novel amylolytic/xylanolytic/cellulolytic multienzyme complex from Clostridium manihotivorum that hydrolyzes polysaccharides in cassava pulp.
34436648	3	78	theme	glycoprotein	432:443	arg1	AXC-MEC					445:451	the glycoprotein AXC-MEC	428:451	the glycoprotein AXC-MEC	428:451	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	3	79	theme	S-500	715:719	arg1	chromatography					736:749	a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography	672:749	chromatography	736:749	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	7	80	theme	genomic	1293:1299	arg1	analysis					1301:1308	the genomic analysis	1289:1308	the genomic analysis of C. manihotivorum CT4	1289:1332	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	1	81	theme	multienzyme	265:275	arg1	MECs					288:291	MECs	288:291	MECs	288:291	Some anaerobic bacteria, particularly Clostridium species, produce extracellular cellulolytic and xylanolytic enzymes as multienzyme complexes (MECs).
34436648	1	81	theme	multienzyme	265:275	arg1	complexes					277:285	multienzyme complexes	265:285	multienzyme complexes (MECs)	265:292	Some anaerobic bacteria, particularly Clostridium species, produce extracellular cellulolytic and xylanolytic enzymes as multienzyme complexes (MECs).
34436648	7	82	theme	Clostridium	1436:1446	arg1	cohesin					1490:1496	cohesin	1490:1496	cohesin	1490:1496	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	7	82	theme	Clostridium	1436:1446	arg1	cellulosomes					1457:1468	cellulosomes	1457:1468	cellulosomes such as scaffoldin, cohesin, and dockerin	1457:1510	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	7	82	theme	Clostridium	1436:1446	arg1	scaffoldin					1478:1487	scaffoldin	1478:1487	scaffoldin	1478:1487	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	7	82	theme	Clostridium	1436:1446	arg1	species					1448:1454	Clostridium species	1436:1454	Clostridium species	1436:1454	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	7	82	theme	Clostridium	1436:1446	arg1	dockerin					1503:1510	dockerin	1503:1510	dockerin	1503:1510	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	3	83	theme	cellulolytic	507:518	arg1	enzymes					520:526	amylolytic, xylanolytic, and cellulolytic enzymes	478:526	amylolytic, xylanolytic, and cellulolytic enzymes	478:526	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	7	84	from	species	1448:1454	arg1	complexes					1421:1429	the well-known multienzyme complexes	1394:1429	the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin	1394:1510	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	7	84	from	species	1448:1454	arg1	components					1380:1389	the known structural components	1359:1389	the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin	1359:1510	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	7	85	theme	complexes	1421:1429	arg1	components					1380:1389	the known structural components	1359:1389	the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin	1359:1510	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	8	86	theme	value-added	1730:1740	arg1	products					1742:1749	value-added products	1730:1749	value-added products	1730:1749	These results suggest that AXC-MEC from C. manihotivorum CT4 is a new MEC capable of hydrolyzing cassava pulp into value-added products, which will benefit the starch industry.
34436648	3	87	theme	crude	547:551	arg1	enzyme					567:572	crude extracellular enzyme	547:572	crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp	547:663	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	7	88	theme	well-known	1398:1407	arg1	complexes					1421:1429	the well-known multienzyme complexes	1394:1429	the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin	1394:1510	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
34436648	3	89	theme	filtration	725:734	arg1	chromatography					736:749	a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography	672:749	chromatography	736:749	In this work, the glycoprotein AXC-MEC, composed of subunits of amylolytic, xylanolytic, and cellulolytic enzymes, was isolated from crude extracellular enzyme of the mesophilic anaerobic bacterium Clostridium manihotivorum CT4, grown on cassava pulp, using a milled cassava pulp column and Sephacryl S-500 gel filtration chromatography.
34436648	11	90	theme	hydrolysis	1989:1998	arg1	products					2000:2007	hydrolysis products	1989:2007	hydrolysis products from cassava pulp by AXC-MEC	1989:2036	• Glucose and oligosaccharides were hydrolysis products from cassava pulp by AXC-MEC.
34436648	6	91	attach	present	1187:1193	arg1	pulp					1206:1209	cassava pulp	1198:1209	cassava pulp	1198:1209	The AXC-MEC is highly capable of degrading starch and non-starch polysaccharides present in cassava pulp into glucose and oligosaccharides, without conventional pretreatment.
34436648	6	91	attach	present	1187:1193	arg2	polysaccharides					1171:1185	starch and non-starch polysaccharides	1149:1185	starch and non-starch polysaccharides present in cassava pulp	1149:1209	The AXC-MEC is highly capable of degrading starch and non-starch polysaccharides present in cassava pulp into glucose and oligosaccharides, without conventional pretreatment.
34436648	7	92	theme	manihotivorum	1316:1328	arg1	CT4					1330:1332	C. manihotivorum CT4	1313:1332	C. manihotivorum CT4	1313:1332	Base on the genomic analysis of C. manihotivorum CT4, we found no evidence of the known structural components of the well-known multienzyme complexes from Clostridium species, cellulosomes such as scaffoldin, cohesin, and dockerin, indicating that AXC-MEC from strain CT4 exhibit a different manner of assembly from the cellulosomes.
33605540	6	0	theme	DOX	1194:1196	arg1	release					1183:1189	controlled and sustained release	1158:1189	controlled and sustained release of DOX	1158:1196	Drug loading and release profile data showed that APT-GO-CO-γ-PGA is provided with high drug loading capacity and is capable of controlled and sustained release of DOX.
33605540	7	1	theme	cells	1331:1335	arg1	surface					1312:1318	the surface	1308:1318	the surface of Beas-2B cells	1308:1335	Cell experimental results indicated that since C23 was overexpressed on the surface of Hela cells but not on the surface of Beas-2B cells, APT-GO-CO-γ-PGA-DOX can target Hela cells and make increase toxicity to Hela cells than Beas-2B cells, and the IC50 value of APT-GO-CO-γ-PGA-DOX was 3.23±0.04 μg/mL.
33605540	6	2	theme	release	1183:1189	arg1	capable					1147:1153	capable	1147:1153	capable	1147:1153	Drug loading and release profile data showed that APT-GO-CO-γ-PGA is provided with high drug loading capacity and is capable of controlled and sustained release of DOX.
33605540	7	3	theme	Hela	1369:1372	arg1	cells					1374:1378	Hela cells	1369:1378	Hela cells	1369:1378	Cell experimental results indicated that since C23 was overexpressed on the surface of Hela cells but not on the surface of Beas-2B cells, APT-GO-CO-γ-PGA-DOX can target Hela cells and make increase toxicity to Hela cells than Beas-2B cells, and the IC50 value of APT-GO-CO-γ-PGA-DOX was 3.23±0.04 μg/mL.
33605540	4	4	theme	nucleolin	746:754	arg1	APT					729:731	APT	729:731	APT	729:731	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	4	4	theme	nucleolin	746:754	arg1	-AS1411					720:726	the nucleic acid aptamer NH2 -AS1411	691:726	the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23)	691:760	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	3	5	from	effects	588:594	arg1	organs					617:622	organs	617:622	organs	617:622	The system was not equipped with the ability of initiative targeting, thus resulting into toxicity and side effects on normal tissues or organs.
33605540	3	5	from	effects	588:594	arg1	tissues					606:612	normal tissues	599:612	normal tissues	599:612	The system was not equipped with the ability of initiative targeting, thus resulting into toxicity and side effects on normal tissues or organs.
33605540	6	6	theme	profile	1055:1061	arg1	data					1063:1066	release profile data	1047:1066	release profile data	1047:1066	Drug loading and release profile data showed that APT-GO-CO-γ-PGA is provided with high drug loading capacity and is capable of controlled and sustained release of DOX.
33605540	5	7	theme	complex	963:969	arg1	properties					933:942	The structure, composition, dispersion, particle size and morphology properties	864:942	The structure, composition, dispersion, particle size and morphology properties of the synthesized complex	864:969	The structure, composition, dispersion, particle size and morphology properties of the synthesized complex have been studied using multiple characterization methods.
33605540	4	8	theme	nano	821:824	arg1	system					840:845	the targeted nano drug delivery system	808:845	the targeted nano drug delivery system APT-GO-CO-γ-PGA	808:861	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	7	9	theme	cells	1291:1295	arg1	surface					1275:1281	the surface	1271:1281	the surface of Hela cells	1271:1295	Cell experimental results indicated that since C23 was overexpressed on the surface of Hela cells but not on the surface of Beas-2B cells, APT-GO-CO-γ-PGA-DOX can target Hela cells and make increase toxicity to Hela cells than Beas-2B cells, and the IC50 value of APT-GO-CO-γ-PGA-DOX was 3.23±0.04 μg/mL.
33605540	3	10	theme	targeting	539:547	arg1	ability					517:523	the ability	513:523	the ability of initiative targeting	513:547	The system was not equipped with the ability of initiative targeting, thus resulting into toxicity and side effects on normal tissues or organs.
33605540	2	11	theme	oligosaccharide	210:224	arg1	materials					188:196	Biological materials	177:196	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA)	177:261	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	2	11	theme	oligosaccharide	210:224	arg1	modifiers					296:304	modifiers	296:304	modifiers	296:304	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	8	12	theme	targeted	1662:1669	arg1	carrier					1671:1677	the targeted carrier	1658:1677	the targeted carrier	1658:1677	All results proved that APT-GO-CO-γ-PGA can deliver antitumor drugs in a targeted manner, and achieve the effect of reducing poison, which indicated that the targeted carrier exhibits a broad application prospect in the field of biomedicine.
33605540	6	13	theme	loading	1123:1129	arg1	capacity					1131:1138	high drug loading capacity	1113:1138	high drug loading capacity	1113:1138	Drug loading and release profile data showed that APT-GO-CO-γ-PGA is provided with high drug loading capacity and is capable of controlled and sustained release of DOX.
33605540	2	14	theme	acid	250:253	arg1	materials					188:196	Biological materials	177:196	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA)	177:261	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	2	14	theme	acid	250:253	arg1	modifiers					296:304	modifiers	296:304	modifiers	296:304	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	2	15	theme	based	444:448	arg1	GO-CO-γ-PGA-DOX					462:476	GO-CO-γ-PGA-DOX	462:476	GO-CO-γ-PGA-DOX	462:476	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	2	15	theme	based	444:448	arg1	composites					450:459	graphene oxide based composites	429:459	graphene oxide based composites (GO-CO-γ-PGA-DOX)	429:477	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	6	16	theme	high	1113:1116	arg1	capacity					1131:1138	high drug loading capacity	1113:1138	high drug loading capacity	1113:1138	Drug loading and release profile data showed that APT-GO-CO-γ-PGA is provided with high drug loading capacity and is capable of controlled and sustained release of DOX.
33605540	0	17	theme	Functionalized	47:60	arg1	Composites					83:92	AS1411 Functionalized Graphene Oxide Based Composites	40:92	AS1411 Functionalized Graphene Oxide Based Composites	40:92	A Targeted Nano Drug Delivery System of AS1411 Functionalized Graphene Oxide Based Composites.
33605540	8	18	theme	broad	1690:1694	arg1	prospect					1708:1715	a broad application prospect	1688:1715	a broad application prospect	1688:1715	All results proved that APT-GO-CO-γ-PGA can deliver antitumor drugs in a targeted manner, and achieve the effect of reducing poison, which indicated that the targeted carrier exhibits a broad application prospect in the field of biomedicine.
33605540	1	19	theme	antitumor	133:141	arg1	vehicles					148:155	antitumor drug vehicles	133:155	antitumor drug vehicles	133:155	A novel method for the preparation of antitumor drug vehicles has been optimized.
33605540	7	20	theme	APT-GO-CO-γ-PGA-DOX	1463:1481	arg1	value					1454:1458	the IC50 value	1445:1458	the IC50 value of APT-GO-CO-γ-PGA-DOX	1445:1481	Cell experimental results indicated that since C23 was overexpressed on the surface of Hela cells but not on the surface of Beas-2B cells, APT-GO-CO-γ-PGA-DOX can target Hela cells and make increase toxicity to Hela cells than Beas-2B cells, and the IC50 value of APT-GO-CO-γ-PGA-DOX was 3.23±0.04 μg/mL.
33605540	7	20	theme	APT-GO-CO-γ-PGA-DOX	1463:1481	arg1	3.23±0.04 μg/mL					1487:1501	3.23±0.04 μg/mL	1487:1501	3.23±0.04 μg/mL	1487:1501	Cell experimental results indicated that since C23 was overexpressed on the surface of Hela cells but not on the surface of Beas-2B cells, APT-GO-CO-γ-PGA-DOX can target Hela cells and make increase toxicity to Hela cells than Beas-2B cells, and the IC50 value of APT-GO-CO-γ-PGA-DOX was 3.23±0.04 μg/mL.
33605540	2	21	theme	graphene	429:436	arg1	GO-CO-γ-PGA-DOX					462:476	GO-CO-γ-PGA-DOX	462:476	GO-CO-γ-PGA-DOX	462:476	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	2	21	theme	graphene	429:436	arg1	composites					450:459	graphene oxide based composites	429:459	graphene oxide based composites (GO-CO-γ-PGA-DOX)	429:477	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	0	22	theme	Oxide	71:75	arg1	Composites					83:92	AS1411 Functionalized Graphene Oxide Based Composites	40:92	AS1411 Functionalized Graphene Oxide Based Composites	40:92	A Targeted Nano Drug Delivery System of AS1411 Functionalized Graphene Oxide Based Composites.
33605540	1	23	theme	vehicles	148:155	arg1	preparation					118:128	the preparation	114:128	the preparation of antitumor drug vehicles	114:155	A novel method for the preparation of antitumor drug vehicles has been optimized.
33605540	4	24	theme	delivery	831:838	arg1	system					840:845	the targeted nano drug delivery system	808:845	the targeted nano drug delivery system APT-GO-CO-γ-PGA	808:861	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	0	25	theme	Composites	83:92	arg1	System					30:35	A Targeted Nano Drug Delivery System	0:35	A Targeted Nano Drug Delivery System of AS1411 Functionalized Graphene Oxide Based Composites.	0:93	A Targeted Nano Drug Delivery System of AS1411 Functionalized Graphene Oxide Based Composites.
33605540	4	26	theme	nucleic	695:701	arg1	APT					729:731	APT	729:731	APT	729:731	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	4	26	theme	nucleic	695:701	arg1	-AS1411					720:726	the nucleic acid aptamer NH2 -AS1411	691:726	the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23)	691:760	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	5	27	theme	characterization	1004:1019	arg1	methods					1021:1027	multiple characterization methods	995:1027	multiple characterization methods	995:1027	The structure, composition, dispersion, particle size and morphology properties of the synthesized complex have been studied using multiple characterization methods.
33605540	4	28	used	used	766:769	arg2	APT					729:731	APT	729:731	APT	729:731	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	4	28	used	used	766:769	arg2	-AS1411					720:726	the nucleic acid aptamer NH2 -AS1411	691:726	the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23)	691:760	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	5	29	theme	synthesized	951:961	arg1	complex					963:969	the synthesized complex	947:969	the synthesized complex	947:969	The structure, composition, dispersion, particle size and morphology properties of the synthesized complex have been studied using multiple characterization methods.
33605540	0	30	theme	Nano	11:14	arg1	System					30:35	A Targeted Nano Drug Delivery System	0:35	A Targeted Nano Drug Delivery System of AS1411 Functionalized Graphene Oxide Based Composites.	0:93	A Targeted Nano Drug Delivery System of AS1411 Functionalized Graphene Oxide Based Composites.
33605540	1	31	theme	novel	97:101	arg1	method					103:108	A novel method	95:108	A novel method for the preparation of antitumor drug vehicles	95:155	A novel method for the preparation of antitumor drug vehicles has been optimized.
33605540	0	32	theme	Delivery	21:28	arg1	System					30:35	A Targeted Nano Drug Delivery System	0:35	A Targeted Nano Drug Delivery System of AS1411 Functionalized Graphene Oxide Based Composites.	0:93	A Targeted Nano Drug Delivery System of AS1411 Functionalized Graphene Oxide Based Composites.
33605540	5	33	theme	size	913:916	arg1	properties					933:942	The structure, composition, dispersion, particle size and morphology properties	864:942	The structure, composition, dispersion, particle size and morphology properties of the synthesized complex	864:969	The structure, composition, dispersion, particle size and morphology properties of the synthesized complex have been studied using multiple characterization methods.
33605540	7	34	theme	experimental	1204:1215	arg1	results					1217:1223	Cell experimental results	1199:1223	Cell experimental results	1199:1223	Cell experimental results indicated that since C23 was overexpressed on the surface of Hela cells but not on the surface of Beas-2B cells, APT-GO-CO-γ-PGA-DOX can target Hela cells and make increase toxicity to Hela cells than Beas-2B cells, and the IC50 value of APT-GO-CO-γ-PGA-DOX was 3.23±0.04 μg/mL.
33605540	2	35	theme	drug	404:407	arg1	systems					418:424	a nano drug delivery systems	397:424	a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX)	397:477	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	2	36	theme	graphene	327:334	arg1	oxide					336:340	graphene oxide	327:340	graphene oxide (GO)	327:345	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	7	37	theme	Hela	1410:1413	arg1	cells					1415:1419	Hela cells	1410:1419	Hela cells	1410:1419	Cell experimental results indicated that since C23 was overexpressed on the surface of Hela cells but not on the surface of Beas-2B cells, APT-GO-CO-γ-PGA-DOX can target Hela cells and make increase toxicity to Hela cells than Beas-2B cells, and the IC50 value of APT-GO-CO-γ-PGA-DOX was 3.23±0.04 μg/mL.
33605540	5	38	theme	morphology	922:931	arg1	properties					933:942	The structure, composition, dispersion, particle size and morphology properties	864:942	The structure, composition, dispersion, particle size and morphology properties of the synthesized complex	864:969	The structure, composition, dispersion, particle size and morphology properties of the synthesized complex have been studied using multiple characterization methods.
33605540	4	39	theme	aptamer	708:714	arg1	APT					729:731	APT	729:731	APT	729:731	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	4	39	theme	aptamer	708:714	arg1	-AS1411					720:726	the nucleic acid aptamer NH2 -AS1411	691:726	the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23)	691:760	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	8	40	theme	antitumor	1556:1564	arg1	drugs					1566:1570	antitumor drugs	1556:1570	antitumor drugs	1556:1570	All results proved that APT-GO-CO-γ-PGA can deliver antitumor drugs in a targeted manner, and achieve the effect of reducing poison, which indicated that the targeted carrier exhibits a broad application prospect in the field of biomedicine.
33605540	3	41	theme	normal	599:604	arg1	tissues					606:612	normal tissues	599:612	normal tissues	599:612	The system was not equipped with the ability of initiative targeting, thus resulting into toxicity and side effects on normal tissues or organs.
33605540	8	42	theme	targeted	1577:1584	arg1	manner					1586:1591	a targeted manner	1575:1591	a targeted manner	1575:1591	All results proved that APT-GO-CO-γ-PGA can deliver antitumor drugs in a targeted manner, and achieve the effect of reducing poison, which indicated that the targeted carrier exhibits a broad application prospect in the field of biomedicine.
33605540	3	43	from	toxicity	570:577	arg1	organs					617:622	organs	617:622	organs	617:622	The system was not equipped with the ability of initiative targeting, thus resulting into toxicity and side effects on normal tissues or organs.
33605540	3	43	from	toxicity	570:577	arg1	tissues					606:612	normal tissues	599:612	normal tissues	599:612	The system was not equipped with the ability of initiative targeting, thus resulting into toxicity and side effects on normal tissues or organs.
33605540	6	44	theme	sustained	1173:1181	arg1	release					1183:1189	controlled and sustained release	1158:1189	controlled and sustained release of DOX	1158:1196	Drug loading and release profile data showed that APT-GO-CO-γ-PGA is provided with high drug loading capacity and is capable of controlled and sustained release of DOX.
33605540	4	45	theme	targeted	737:744	arg1	C23					757:759	C23	757:759	C23	757:759	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	4	45	theme	targeted	737:744	arg1	nucleolin					746:754	targeted nucleolin	737:754	targeted nucleolin (C23)	737:760	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	7	46	theme	Beas-2B	1323:1329	arg1	cells					1331:1335	Beas-2B cells	1323:1335	Beas-2B cells	1323:1335	Cell experimental results indicated that since C23 was overexpressed on the surface of Hela cells but not on the surface of Beas-2B cells, APT-GO-CO-γ-PGA-DOX can target Hela cells and make increase toxicity to Hela cells than Beas-2B cells, and the IC50 value of APT-GO-CO-γ-PGA-DOX was 3.23±0.04 μg/mL.
33605540	6	47	theme	controlled	1158:1167	arg1	release					1183:1189	controlled and sustained release	1158:1189	controlled and sustained release of DOX	1158:1196	Drug loading and release profile data showed that APT-GO-CO-γ-PGA is provided with high drug loading capacity and is capable of controlled and sustained release of DOX.
33605540	6	48	theme	release	1047:1053	arg1	data					1063:1066	release profile data	1047:1066	release profile data	1047:1066	Drug loading and release profile data showed that APT-GO-CO-γ-PGA is provided with high drug loading capacity and is capable of controlled and sustained release of DOX.
33605540	3	49	theme	initiative	528:537	arg1	targeting					539:547	initiative targeting	528:547	initiative targeting	528:547	The system was not equipped with the ability of initiative targeting, thus resulting into toxicity and side effects on normal tissues or organs.
33605540	4	50	theme	targeted	812:819	arg1	system					840:845	the targeted nano drug delivery system	808:845	the targeted nano drug delivery system APT-GO-CO-γ-PGA	808:861	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	7	51	theme	Hela	1286:1289	arg1	cells					1291:1295	Hela cells	1286:1295	Hela cells	1286:1295	Cell experimental results indicated that since C23 was overexpressed on the surface of Hela cells but not on the surface of Beas-2B cells, APT-GO-CO-γ-PGA-DOX can target Hela cells and make increase toxicity to Hela cells than Beas-2B cells, and the IC50 value of APT-GO-CO-γ-PGA-DOX was 3.23±0.04 μg/mL.
33605540	6	52	theme	drug	1118:1121	arg1	capacity					1131:1138	high drug loading capacity	1113:1138	high drug loading capacity	1113:1138	Drug loading and release profile data showed that APT-GO-CO-γ-PGA is provided with high drug loading capacity and is capable of controlled and sustained release of DOX.
33605540	0	53	theme	Graphene	62:69	arg1	Composites					83:92	AS1411 Functionalized Graphene Oxide Based Composites	40:92	AS1411 Functionalized Graphene Oxide Based Composites	40:92	A Targeted Nano Drug Delivery System of AS1411 Functionalized Graphene Oxide Based Composites.
33605540	4	54	from	conjugate	774:782	arg1	GO-CO-γ-PGA					787:797	GO-CO-γ-PGA	787:797	GO-CO-γ-PGA	787:797	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	2	55	theme	γ-polyglutamic	235:248	arg1	γ-PGA					256:260	γ-PGA	256:260	γ-PGA	256:260	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	2	55	theme	γ-polyglutamic	235:248	arg1	acid					250:253	γ-polyglutamic acid	235:253	γ-polyglutamic acid (γ-PGA)	235:261	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	2	56	theme	oxide	438:442	arg1	GO-CO-γ-PGA-DOX					462:476	GO-CO-γ-PGA-DOX	462:476	GO-CO-γ-PGA-DOX	462:476	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	2	56	theme	oxide	438:442	arg1	composites					450:459	graphene oxide based composites	429:459	graphene oxide based composites (GO-CO-γ-PGA-DOX)	429:477	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	0	57	theme	Based	77:81	arg1	Composites					83:92	AS1411 Functionalized Graphene Oxide Based Composites	40:92	AS1411 Functionalized Graphene Oxide Based Composites	40:92	A Targeted Nano Drug Delivery System of AS1411 Functionalized Graphene Oxide Based Composites.
33605540	1	58	theme	drug	143:146	arg1	vehicles					148:155	antitumor drug vehicles	133:155	antitumor drug vehicles	133:155	A novel method for the preparation of antitumor drug vehicles has been optimized.
33605540	7	59	theme	IC50	1449:1452	arg1	value					1454:1458	the IC50 value	1445:1458	the IC50 value of APT-GO-CO-γ-PGA-DOX	1445:1481	Cell experimental results indicated that since C23 was overexpressed on the surface of Hela cells but not on the surface of Beas-2B cells, APT-GO-CO-γ-PGA-DOX can target Hela cells and make increase toxicity to Hela cells than Beas-2B cells, and the IC50 value of APT-GO-CO-γ-PGA-DOX was 3.23±0.04 μg/mL.
33605540	7	59	theme	IC50	1449:1452	arg1	3.23±0.04 μg/mL					1487:1501	3.23±0.04 μg/mL	1487:1501	3.23±0.04 μg/mL	1487:1501	Cell experimental results indicated that since C23 was overexpressed on the surface of Hela cells but not on the surface of Beas-2B cells, APT-GO-CO-γ-PGA-DOX can target Hela cells and make increase toxicity to Hela cells than Beas-2B cells, and the IC50 value of APT-GO-CO-γ-PGA-DOX was 3.23±0.04 μg/mL.
33605540	8	60	theme	application	1696:1706	arg1	prospect					1708:1715	a broad application prospect	1688:1715	a broad application prospect	1688:1715	All results proved that APT-GO-CO-γ-PGA can deliver antitumor drugs in a targeted manner, and achieve the effect of reducing poison, which indicated that the targeted carrier exhibits a broad application prospect in the field of biomedicine.
33605540	4	61	theme	drug	826:829	arg1	system					840:845	the targeted nano drug delivery system	808:845	the targeted nano drug delivery system APT-GO-CO-γ-PGA	808:861	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	2	62	dep	oxide	336:340	arg1	GO					343:344	GO	343:344	GO	343:344	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	4	63	theme	system	683:688	arg1	property					667:674	the targeting property	653:674	the targeting property of the system	653:688	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	5	64	theme	multiple	995:1002	arg1	methods					1021:1027	multiple characterization methods	995:1027	multiple characterization methods	995:1027	The structure, composition, dispersion, particle size and morphology properties of the synthesized complex have been studied using multiple characterization methods.
33605540	5	65	theme	structure	868:876	arg1	properties					933:942	The structure, composition, dispersion, particle size and morphology properties	864:942	The structure, composition, dispersion, particle size and morphology properties of the synthesized complex	864:969	The structure, composition, dispersion, particle size and morphology properties of the synthesized complex have been studied using multiple characterization methods.
33605540	4	66	theme	system	840:845	arg1	APT-GO-CO-γ-PGA					847:861	the targeted nano drug delivery system APT-GO-CO-γ-PGA	808:861	the targeted nano drug delivery system APT-GO-CO-γ-PGA	808:861	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	0	67	theme	Targeted	2:9	arg1	System					30:35	A Targeted Nano Drug Delivery System	0:35	A Targeted Nano Drug Delivery System of AS1411 Functionalized Graphene Oxide Based Composites.	0:93	A Targeted Nano Drug Delivery System of AS1411 Functionalized Graphene Oxide Based Composites.
33605540	2	68	theme	Biological	177:186	arg1	materials					188:196	Biological materials	177:196	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA)	177:261	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	2	68	theme	Biological	177:186	arg1	modifiers					296:304	modifiers	296:304	modifiers	296:304	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	4	69	theme	targeting	657:665	arg1	property					667:674	the targeting property	653:674	the targeting property of the system	653:688	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	0	70	theme	Drug	16:19	arg1	System					30:35	A Targeted Nano Drug Delivery System	0:35	A Targeted Nano Drug Delivery System of AS1411 Functionalized Graphene Oxide Based Composites.	0:93	A Targeted Nano Drug Delivery System of AS1411 Functionalized Graphene Oxide Based Composites.
33605540	8	71	theme	biomedicine	1733:1743	arg1	field					1724:1728	the field	1720:1728	the field of biomedicine	1720:1743	All results proved that APT-GO-CO-γ-PGA can deliver antitumor drugs in a targeted manner, and achieve the effect of reducing poison, which indicated that the targeted carrier exhibits a broad application prospect in the field of biomedicine.
33605540	3	72	theme	side	583:586	arg1	effects					588:594	side effects	583:594	side effects	583:594	The system was not equipped with the ability of initiative targeting, thus resulting into toxicity and side effects on normal tissues or organs.
33605540	5	73	theme	particle	904:911	arg1	size					913:916	particle size	904:916	particle size	904:916	The structure, composition, dispersion, particle size and morphology properties of the synthesized complex have been studied using multiple characterization methods.
33605540	7	74	theme	Cell	1199:1202	arg1	results					1217:1223	Cell experimental results	1199:1223	Cell experimental results	1199:1223	Cell experimental results indicated that since C23 was overexpressed on the surface of Hela cells but not on the surface of Beas-2B cells, APT-GO-CO-γ-PGA-DOX can target Hela cells and make increase toxicity to Hela cells than Beas-2B cells, and the IC50 value of APT-GO-CO-γ-PGA-DOX was 3.23±0.04 μg/mL.
33605540	2	75	theme	delivery	409:416	arg1	systems					418:424	a nano drug delivery systems	397:424	a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX)	397:477	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	2	76	theme	composites	450:459	arg1	systems					418:424	a nano drug delivery systems	397:424	a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX)	397:477	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	0	77	theme	AS1411	40:45	arg1	Composites					83:92	AS1411 Functionalized Graphene Oxide Based Composites	40:92	AS1411 Functionalized Graphene Oxide Based Composites	40:92	A Targeted Nano Drug Delivery System of AS1411 Functionalized Graphene Oxide Based Composites.
33605540	7	78	theme	Beas-2B	1426:1432	arg1	cells					1434:1438	Beas-2B cells	1426:1438	Beas-2B cells	1426:1438	Cell experimental results indicated that since C23 was overexpressed on the surface of Hela cells but not on the surface of Beas-2B cells, APT-GO-CO-γ-PGA-DOX can target Hela cells and make increase toxicity to Hela cells than Beas-2B cells, and the IC50 value of APT-GO-CO-γ-PGA-DOX was 3.23±0.04 μg/mL.
33605540	2	79	theme	nano	399:402	arg1	systems					418:424	a nano drug delivery systems	397:424	a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX)	397:477	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	2	80	theme	chitosan	201:208	arg1	CO					227:228	CO	227:228	CO	227:228	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	2	80	theme	chitosan	201:208	arg1	oligosaccharide					210:224	chitosan oligosaccharide	201:224	chitosan oligosaccharide (CO)	201:229	Biological materials of chitosan oligosaccharide (CO) and γ-polyglutamic acid (γ-PGA) have previously been employed as modifiers to covalently modify graphene oxide (GO), which in turn loaded doxorubicin (DOX) to obtain a nano drug delivery systems of graphene oxide based composites (GO-CO-γ-PGA-DOX).
33605540	5	81	theme	composition	879:889	arg1	properties					933:942	The structure, composition, dispersion, particle size and morphology properties	864:942	The structure, composition, dispersion, particle size and morphology properties of the synthesized complex	864:969	The structure, composition, dispersion, particle size and morphology properties of the synthesized complex have been studied using multiple characterization methods.
33605540	6	82	theme	Drug	1030:1033	arg1	loading					1035:1041	Drug loading	1030:1041	Drug loading	1030:1041	Drug loading and release profile data showed that APT-GO-CO-γ-PGA is provided with high drug loading capacity and is capable of controlled and sustained release of DOX.
33605540	4	83	theme	acid	703:706	arg1	APT					729:731	APT	729:731	APT	729:731	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	4	83	theme	acid	703:706	arg1	-AS1411					720:726	the nucleic acid aptamer NH2 -AS1411	691:726	the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23)	691:760	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	5	84	theme	dispersion	892:901	arg1	properties					933:942	The structure, composition, dispersion, particle size and morphology properties	864:942	The structure, composition, dispersion, particle size and morphology properties of the synthesized complex	864:969	The structure, composition, dispersion, particle size and morphology properties of the synthesized complex have been studied using multiple characterization methods.
33605540	4	85	theme	NH2	716:718	arg1	APT					729:731	APT	729:731	APT	729:731	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
33605540	4	85	theme	NH2	716:718	arg1	-AS1411					720:726	the nucleic acid aptamer NH2 -AS1411	691:726	the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23)	691:760	In order to further improve the targeting property of the system, the nucleic acid aptamer NH2 -AS1411 (APT) of targeted nucleolin (C23) was used to conjugate on GO-CO-γ-PGA to yield the targeted nano drug delivery system APT-GO-CO-γ-PGA.
32349995	6	0	theme	eGFR	920:923	arg1	change					925:930	eGFR change	920:930	eGFR change per year	920:939	RESULTS Eleven traits were significantly associated with eGFR change per year.
32349995	7	1	theme	disialylated	990:1001	arg1	structures					1003:1012	fucosylated and fucosylated disialylated structures	962:1012	structures	1003:1012	Bisecting GlcNAc in fucosylated and fucosylated disialylated structures and monosialylation of fucosylated digalactosylated structures were associated with a faster decrease of eGFR.
32349995	7	2	gly	fucosylated	962:972	arg1	structures					1003:1012	fucosylated and fucosylated disialylated structures	962:1012	structures	1003:1012	Bisecting GlcNAc in fucosylated and fucosylated disialylated structures and monosialylation of fucosylated digalactosylated structures were associated with a faster decrease of eGFR.
32349995	10	3	theme	type	1314:1317	arg1	diabetes					1321:1328	type 2 diabetes	1314:1328	type 2 diabetes	1314:1328	CONCLUSIONS In type 2 diabetes, we found IgG N-glycosylation patterns associated with a faster decline of kidney function, reflecting a pro-inflammatory state of IgG.
32349995	4	4	theme	prospective	572:582	arg1	follow-up					584:592	mean prospective follow-up	567:592	mean prospective follow-up of 7.0 years	567:605	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	10	5	dep	CONCLUSIONS	1299:1309	arg1	found					1334:1338	found	1334:1338	found	1334:1338	CONCLUSIONS In type 2 diabetes, we found IgG N-glycosylation patterns associated with a faster decline of kidney function, reflecting a pro-inflammatory state of IgG.
32349995	7	6	theme	fucosylated	962:972	arg1	structures					1003:1012	fucosylated and fucosylated disialylated structures	962:1012	structures	1003:1012	Bisecting GlcNAc in fucosylated and fucosylated disialylated structures and monosialylation of fucosylated digalactosylated structures were associated with a faster decrease of eGFR.
32349995	2	7	theme	IgG	336:338	arg1	potential					323:331	the inflammatory potential	306:331	the inflammatory potential of IgG	306:338	Glycosylation of immunoglobulin G (IgG) is an important post-translation process affecting the inflammatory potential of IgG.
32349995	7	8	from	GlcNAc	952:957	arg1	structures					1003:1012	fucosylated and fucosylated disialylated structures	962:1012	structures	1003:1012	Bisecting GlcNAc in fucosylated and fucosylated disialylated structures and monosialylation of fucosylated digalactosylated structures were associated with a faster decrease of eGFR.
32349995	7	8	from	GlcNAc	952:957	arg1	monosialylation					1018:1032	monosialylation	1018:1032	monosialylation of fucosylated digalactosylated structures	1018:1075	Bisecting GlcNAc in fucosylated and fucosylated disialylated structures and monosialylation of fucosylated digalactosylated structures were associated with a faster decrease of eGFR.
32349995	4	9	theme	mean	567:570	arg1	follow-up					584:592	mean prospective follow-up	567:592	mean prospective follow-up of 7.0 years	567:605	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	10	theme	total	763:767	arg1	follow-up					769:777	total follow-up	763:777	total follow-up	763:777	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	10	11	theme	pro-inflammatory	1435:1450	arg1	state					1452:1456	a pro-inflammatory state	1433:1456	a pro-inflammatory state of IgG	1433:1463	CONCLUSIONS In type 2 diabetes, we found IgG N-glycosylation patterns associated with a faster decline of kidney function, reflecting a pro-inflammatory state of IgG.
32349995	0	12	theme	type	71:74	arg1	diabetes					78:85	type 2 diabetes	71:85	type 2 diabetes	71:85	Association of the IgG N-glycome with the course of kidney function in type 2 diabetes.
32349995	10	13	theme	faster	1387:1392	arg1	decline					1394:1400	a faster decline	1385:1400	a faster decline of kidney function	1385:1419	CONCLUSIONS In type 2 diabetes, we found IgG N-glycosylation patterns associated with a faster decline of kidney function, reflecting a pro-inflammatory state of IgG.
32349995	9	14	theme	significant	1234:1244	arg1	associations					1246:1257	No significant associations	1231:1257	No significant associations between IgG glycans and ACR	1231:1285	No significant associations between IgG glycans and ACR were found.
32349995	2	15	gly	Glycosylation	215:227	arg1	IgG					250:252	IgG	250:252	IgG	250:252	Glycosylation of immunoglobulin G (IgG) is an important post-translation process affecting the inflammatory potential of IgG.
32349995	2	15	gly	Glycosylation	215:227	arg1	G					247:247	immunoglobulin G	232:247	immunoglobulin G (IgG)	232:253	Glycosylation of immunoglobulin G (IgG) is an important post-translation process affecting the inflammatory potential of IgG.
32349995	11	16	theme	renal	1566:1570	arg1	macroangiopathy					1572:1586	renal macroangiopathy	1566:1586	renal macroangiopathy rather than microvascular disease	1566:1620	eGFR, but not ACR, was associated with IgG glycans, which suggests these associations may represent renal macroangiopathy rather than microvascular disease.
32349995	7	17	gly	monosialylation	1018:1032	arg1	structures					1066:1075	fucosylated digalactosylated structures	1037:1075	fucosylated digalactosylated structures	1037:1075	Bisecting GlcNAc in fucosylated and fucosylated disialylated structures and monosialylation of fucosylated digalactosylated structures were associated with a faster decrease of eGFR.
32349995	1	18	theme	function	161:168	arg1	decline					170:176	kidney function decline	154:176	kidney function decline in individuals with type 2 diabetes	154:212	INTRODUCTION Inflammatory processes are thought to be involved in kidney function decline in individuals with type 2 diabetes.
32349995	5	19	theme	multiple	841:848	arg1	comparisons					850:860	multiple comparisons	841:860	multiple comparisons	841:860	Models were adjusted for clinical variables and multiple comparisons.
32349995	3	20	from	relationship	373:384	arg1	diabetes					453:460	type 2 diabetes	446:460	type 2 diabetes	446:460	We investigated the prospective relationship between IgG N-glycosylation patterns and kidney function in type 2 diabetes.
32349995	4	21	from	METHODS	483:489	arg1	study					547:551	an all-lines-of-care case-control study	513:551	an all-lines-of-care case-control study (n=1886)	513:560	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	21	from	METHODS	483:489	arg1	study					506:510	the DiaGene study	494:510	the DiaGene study	494:510	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	8	22	gly	Fucosylation	1125:1136	arg1	structures					1172:1181	neutral and monogalactosylated structures	1141:1181	neutral and monogalactosylated structures	1141:1181	Fucosylation of neutral and monogalactosylated structures was associated with less eGFR decline per year.
32349995	1	23	theme	kidney	154:159	arg1	decline					170:176	kidney function decline	154:176	kidney function decline in individuals with type 2 diabetes	154:212	INTRODUCTION Inflammatory processes are thought to be involved in kidney function decline in individuals with type 2 diabetes.
32349995	7	24	gly	fucosylated	978:988	arg1	structures					1003:1012	fucosylated and fucosylated disialylated structures	962:1012	structures	1003:1012	Bisecting GlcNAc in fucosylated and fucosylated disialylated structures and monosialylation of fucosylated digalactosylated structures were associated with a faster decrease of eGFR.
32349995	7	25	theme	eGFR	1119:1122	arg1	decrease					1107:1114	a faster decrease	1098:1114	a faster decrease of eGFR	1098:1122	Bisecting GlcNAc in fucosylated and fucosylated disialylated structures and monosialylation of fucosylated digalactosylated structures were associated with a faster decrease of eGFR.
32349995	4	26	theme	N-glycan	639:646	arg1	profiles					648:655	58 IgG N-glycan profiles	632:655	58 IgG N-glycan profiles	632:655	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	3	27	theme	kidney	427:432	arg1	function					434:441	kidney function	427:441	kidney function	427:441	We investigated the prospective relationship between IgG N-glycosylation patterns and kidney function in type 2 diabetes.
32349995	4	28	from	DESIGN	472:477	arg1	study					547:551	an all-lines-of-care case-control study	513:551	an all-lines-of-care case-control study (n=1886)	513:560	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	28	from	DESIGN	472:477	arg1	study					506:510	the DiaGene study	494:510	the DiaGene study	494:510	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	7	29	theme	faster	1100:1105	arg1	decrease					1107:1114	a faster decrease	1098:1114	a faster decrease of eGFR	1098:1122	Bisecting GlcNAc in fucosylated and fucosylated disialylated structures and monosialylation of fucosylated digalactosylated structures were associated with a faster decrease of eGFR.
32349995	0	30	theme	IgG	19:21	arg1	N-glycome					23:31	the IgG N-glycome	15:31	the IgG N-glycome	15:31	Association of the IgG N-glycome with the course of kidney function in type 2 diabetes.
32349995	10	31	theme	IgG	1461:1463	arg1	state					1452:1456	a pro-inflammatory state	1433:1456	a pro-inflammatory state of IgG	1433:1463	CONCLUSIONS In type 2 diabetes, we found IgG N-glycosylation patterns associated with a faster decline of kidney function, reflecting a pro-inflammatory state of IgG.
32349995	11	32	theme	microvascular	1600:1612	arg1	disease					1614:1620	microvascular disease	1600:1620	renal macroangiopathy rather than microvascular disease	1566:1620	eGFR, but not ACR, was associated with IgG glycans, which suggests these associations may represent renal macroangiopathy rather than microvascular disease.
32349995	2	33	theme	post-translation	271:286	arg1	Glycosylation					215:227	Glycosylation	215:227	Glycosylation of immunoglobulin G (IgG)	215:253	Glycosylation of immunoglobulin G (IgG) is an important post-translation process affecting the inflammatory potential of IgG.
32349995	2	33	theme	post-translation	271:286	arg1	process					288:294	an important post-translation process	258:294	an important post-translation process affecting the inflammatory potential of IgG	258:338	Glycosylation of immunoglobulin G (IgG) is an important post-translation process affecting the inflammatory potential of IgG.
32349995	3	34	theme	prospective	361:371	arg1	relationship					373:384	the prospective relationship	357:384	the prospective relationship between IgG N-glycosylation patterns and kidney function in type 2 diabetes	357:460	We investigated the prospective relationship between IgG N-glycosylation patterns and kidney function in type 2 diabetes.
32349995	7	35	gly	disialylated	990:1001	arg1	structures					1003:1012	fucosylated and fucosylated disialylated structures	962:1012	structures	1003:1012	Bisecting GlcNAc in fucosylated and fucosylated disialylated structures and monosialylation of fucosylated digalactosylated structures were associated with a faster decrease of eGFR.
32349995	6	36	theme	RESULTS	863:869	arg1	traits					878:883	RESULTS Eleven traits	863:883	RESULTS Eleven traits	863:883	RESULTS Eleven traits were significantly associated with eGFR change per year.
32349995	2	37	theme	important	261:269	arg1	Glycosylation					215:227	Glycosylation	215:227	Glycosylation of immunoglobulin G (IgG)	215:253	Glycosylation of immunoglobulin G (IgG) is an important post-translation process affecting the inflammatory potential of IgG.
32349995	2	37	theme	important	261:269	arg1	process					288:294	an important post-translation process	258:294	an important post-translation process affecting the inflammatory potential of IgG	258:338	Glycosylation of immunoglobulin G (IgG) is an important post-translation process affecting the inflammatory potential of IgG.
32349995	10	38	theme	N-glycosylation	1344:1358	arg1	patterns					1360:1367	IgG N-glycosylation patterns	1340:1367	IgG N-glycosylation patterns associated with a faster decline of kidney function	1340:1419	CONCLUSIONS In type 2 diabetes, we found IgG N-glycosylation patterns associated with a faster decline of kidney function, reflecting a pro-inflammatory state of IgG.
32349995	5	39	theme	clinical	818:825	arg1	variables					827:835	clinical variables	818:835	clinical variables	818:835	Models were adjusted for clinical variables and multiple comparisons.
32349995	4	40	theme	estimated	661:669	arg1	rate					693:696	estimated glomerular filtration rate	661:696	estimated glomerular filtration rate (eGFR)	661:703	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	40	theme	estimated	661:669	arg1	eGFR					699:702	eGFR	699:702	eGFR	699:702	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	10	41	theme	IgG	1340:1342	arg1	patterns					1360:1367	IgG N-glycosylation patterns	1340:1367	IgG N-glycosylation patterns associated with a faster decline of kidney function	1340:1419	CONCLUSIONS In type 2 diabetes, we found IgG N-glycosylation patterns associated with a faster decline of kidney function, reflecting a pro-inflammatory state of IgG.
32349995	4	42	theme	IgG	635:637	arg1	profiles					648:655	58 IgG N-glycan profiles	632:655	58 IgG N-glycan profiles	632:655	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	0	43	theme	N-glycome	23:31	arg1	Association					0:10	Association	0:10	Association of the IgG N-glycome with the course of kidney	0:57	Association of the IgG N-glycome with the course of kidney function in type 2 diabetes.
32349995	4	44	theme	all-lines-of-care	516:532	arg1	n=1886					554:559	n=1886	554:559	n=1886	554:559	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	44	theme	all-lines-of-care	516:532	arg1	study					547:551	an all-lines-of-care case-control study	513:551	an all-lines-of-care case-control study (n=1886)	513:560	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	44	theme	all-lines-of-care	516:532	arg1	study					506:510	the DiaGene study	494:510	the DiaGene study	494:510	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	1	45	with	individuals	181:191	arg1	diabetes					205:212	type 2 diabetes	198:212	type 2 diabetes	198:212	INTRODUCTION Inflammatory processes are thought to be involved in kidney function decline in individuals with type 2 diabetes.
32349995	4	46	theme	RESEARCH	463:470	arg1	DESIGN					472:477	RESEARCH DESIGN	463:477	RESEARCH DESIGN	463:477	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	7	47	theme	fucosylated	1037:1047	arg1	structures					1066:1075	fucosylated digalactosylated structures	1037:1075	fucosylated digalactosylated structures	1037:1075	Bisecting GlcNAc in fucosylated and fucosylated disialylated structures and monosialylation of fucosylated digalactosylated structures were associated with a faster decrease of eGFR.
32349995	1	48	theme	type	198:201	arg1	diabetes					205:212	type 2 diabetes	198:212	type 2 diabetes	198:212	INTRODUCTION Inflammatory processes are thought to be involved in kidney function decline in individuals with type 2 diabetes.
32349995	3	49	theme	IgG	394:396	arg1	patterns					414:421	IgG N-glycosylation patterns	394:421	IgG N-glycosylation patterns	394:421	We investigated the prospective relationship between IgG N-glycosylation patterns and kidney function in type 2 diabetes.
32349995	7	50	gly	fucosylated	1037:1047	arg1	structures					1066:1075	fucosylated digalactosylated structures	1037:1075	fucosylated digalactosylated structures	1037:1075	Bisecting GlcNAc in fucosylated and fucosylated disialylated structures and monosialylation of fucosylated digalactosylated structures were associated with a faster decrease of eGFR.
32349995	7	51	theme	digalactosylated	1049:1064	arg1	structures					1066:1075	fucosylated digalactosylated structures	1037:1075	fucosylated digalactosylated structures	1037:1075	Bisecting GlcNAc in fucosylated and fucosylated disialylated structures and monosialylation of fucosylated digalactosylated structures were associated with a faster decrease of eGFR.
32349995	1	52	theme	INTRODUCTION	88:99	arg1	processes					114:122	INTRODUCTION Inflammatory processes	88:122	INTRODUCTION Inflammatory processes	88:122	INTRODUCTION Inflammatory processes are thought to be involved in kidney function decline in individuals with type 2 diabetes.
32349995	3	53	theme	N-glycosylation	398:412	arg1	patterns					414:421	IgG N-glycosylation patterns	394:421	IgG N-glycosylation patterns	394:421	We investigated the prospective relationship between IgG N-glycosylation patterns and kidney function in type 2 diabetes.
32349995	11	54	theme	IgG	1505:1507	arg1	glycans					1509:1515	IgG glycans	1505:1515	IgG glycans	1505:1515	eGFR, but not ACR, was associated with IgG glycans, which suggests these associations may represent renal macroangiopathy rather than microvascular disease.
32349995	8	55	theme	neutral	1141:1147	arg1	structures					1172:1181	neutral and monogalactosylated structures	1141:1181	neutral and monogalactosylated structures	1141:1181	Fucosylation of neutral and monogalactosylated structures was associated with less eGFR decline per year.
32349995	1	56	theme	Inflammatory	101:112	arg1	processes					114:122	INTRODUCTION Inflammatory processes	88:122	INTRODUCTION Inflammatory processes	88:122	INTRODUCTION Inflammatory processes are thought to be involved in kidney function decline in individuals with type 2 diabetes.
32349995	4	57	theme	case-control	534:545	arg1	n=1886					554:559	n=1886	554:559	n=1886	554:559	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	57	theme	case-control	534:545	arg1	study					547:551	an all-lines-of-care case-control study	513:551	an all-lines-of-care case-control study (n=1886)	513:560	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	57	theme	case-control	534:545	arg1	study					506:510	the DiaGene study	494:510	the DiaGene study	494:510	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	7	58	theme	structures	1066:1075	arg1	structures					1003:1012	fucosylated and fucosylated disialylated structures	962:1012	structures	1003:1012	Bisecting GlcNAc in fucosylated and fucosylated disialylated structures and monosialylation of fucosylated digalactosylated structures were associated with a faster decrease of eGFR.
32349995	7	58	theme	structures	1066:1075	arg1	monosialylation					1018:1032	monosialylation	1018:1032	monosialylation of fucosylated digalactosylated structures	1018:1075	Bisecting GlcNAc in fucosylated and fucosylated disialylated structures and monosialylation of fucosylated digalactosylated structures were associated with a faster decrease of eGFR.
32349995	0	59	with	Association	0:10	arg1	course					42:47	the course	38:47	the course of kidney	38:57	Association of the IgG N-glycome with the course of kidney function in type 2 diabetes.
32349995	2	60	theme	inflammatory	310:321	arg1	potential					323:331	the inflammatory potential	306:331	the inflammatory potential of IgG	306:338	Glycosylation of immunoglobulin G (IgG) is an important post-translation process affecting the inflammatory potential of IgG.
32349995	10	61	theme	kidney	1405:1410	arg1	function					1412:1419	kidney function	1405:1419	kidney function	1405:1419	CONCLUSIONS In type 2 diabetes, we found IgG N-glycosylation patterns associated with a faster decline of kidney function, reflecting a pro-inflammatory state of IgG.
32349995	4	62	theme	years	601:605	arg1	follow-up					584:592	mean prospective follow-up	567:592	mean prospective follow-up of 7.0 years	567:605	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	62	theme	years	601:605	arg1	association					612:622	the association	608:622	the association between 58 IgG N-glycan profiles	608:655	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	62	theme	years	601:605	arg1	rate					693:696	estimated glomerular filtration rate	661:696	estimated glomerular filtration rate (eGFR)	661:703	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	62	theme	years	601:605	arg1	ratio					731:735	albumin-to-creatinine ratio	709:735	albumin-to-creatinine ratio (ACR)	709:741	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	62	theme	years	601:605	arg1	ACR					738:740	ACR	738:740	ACR	738:740	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	62	theme	years	601:605	arg1	eGFR					699:702	eGFR	699:702	eGFR	699:702	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	8	63	theme	monogalactosylated	1153:1170	arg1	structures					1172:1181	neutral and monogalactosylated structures	1141:1181	neutral and monogalactosylated structures	1141:1181	Fucosylation of neutral and monogalactosylated structures was associated with less eGFR decline per year.
32349995	2	64	theme	G	247:247	arg1	Glycosylation					215:227	Glycosylation	215:227	Glycosylation of immunoglobulin G (IgG)	215:253	Glycosylation of immunoglobulin G (IgG) is an important post-translation process affecting the inflammatory potential of IgG.
32349995	2	64	theme	G	247:247	arg1	process					288:294	an important post-translation process	258:294	an important post-translation process affecting the inflammatory potential of IgG	258:338	Glycosylation of immunoglobulin G (IgG) is an important post-translation process affecting the inflammatory potential of IgG.
32349995	8	65	theme	structures	1172:1181	arg1	Fucosylation					1125:1136	Fucosylation	1125:1136	Fucosylation of neutral and monogalactosylated structures	1125:1181	Fucosylation of neutral and monogalactosylated structures was associated with less eGFR decline per year.
32349995	4	66	with	METHODS	483:489	arg1	follow-up					584:592	mean prospective follow-up	567:592	mean prospective follow-up of 7.0 years	567:605	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	66	with	METHODS	483:489	arg1	rate					693:696	estimated glomerular filtration rate	661:696	estimated glomerular filtration rate (eGFR)	661:703	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	66	with	METHODS	483:489	arg1	association					612:622	the association	608:622	the association between 58 IgG N-glycan profiles	608:655	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	66	with	METHODS	483:489	arg1	eGFR					699:702	eGFR	699:702	eGFR	699:702	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	67	with	DESIGN	472:477	arg1	follow-up					584:592	mean prospective follow-up	567:592	mean prospective follow-up of 7.0 years	567:605	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	67	with	DESIGN	472:477	arg1	rate					693:696	estimated glomerular filtration rate	661:696	estimated glomerular filtration rate (eGFR)	661:703	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	67	with	DESIGN	472:477	arg1	association					612:622	the association	608:622	the association between 58 IgG N-glycan profiles	608:655	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	67	with	DESIGN	472:477	arg1	eGFR					699:702	eGFR	699:702	eGFR	699:702	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	68	theme	albumin-to-creatinine	709:729	arg1	ACR					738:740	ACR	738:740	ACR	738:740	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	68	theme	albumin-to-creatinine	709:729	arg1	ratio					731:735	albumin-to-creatinine ratio	709:735	albumin-to-creatinine ratio (ACR)	709:741	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	0	69	theme	kidney	52:57	arg1	course					42:47	the course	38:47	the course of kidney	38:57	Association of the IgG N-glycome with the course of kidney function in type 2 diabetes.
32349995	10	70	theme	function	1412:1419	arg1	decline					1394:1400	a faster decline	1385:1400	a faster decline of kidney function	1385:1419	CONCLUSIONS In type 2 diabetes, we found IgG N-glycosylation patterns associated with a faster decline of kidney function, reflecting a pro-inflammatory state of IgG.
32349995	2	71	theme	immunoglobulin	232:245	arg1	IgG					250:252	IgG	250:252	IgG	250:252	Glycosylation of immunoglobulin G (IgG) is an important post-translation process affecting the inflammatory potential of IgG.
32349995	2	71	theme	immunoglobulin	232:245	arg1	G					247:247	immunoglobulin G	232:247	immunoglobulin G (IgG)	232:253	Glycosylation of immunoglobulin G (IgG) is an important post-translation process affecting the inflammatory potential of IgG.
32349995	1	72	from	decline	170:176	arg1	individuals					181:191	individuals	181:191	individuals with type 2 diabetes	181:212	INTRODUCTION Inflammatory processes are thought to be involved in kidney function decline in individuals with type 2 diabetes.
32349995	4	73	theme	filtration	682:691	arg1	rate					693:696	estimated glomerular filtration rate	661:696	estimated glomerular filtration rate (eGFR)	661:703	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	73	theme	filtration	682:691	arg1	eGFR					699:702	eGFR	699:702	eGFR	699:702	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	3	74	theme	type	446:449	arg1	diabetes					453:460	type 2 diabetes	446:460	type 2 diabetes	446:460	We investigated the prospective relationship between IgG N-glycosylation patterns and kidney function in type 2 diabetes.
32349995	9	75	theme	IgG	1267:1269	arg1	glycans					1271:1277	IgG glycans	1267:1277	IgG glycans	1267:1277	No significant associations between IgG glycans and ACR were found.
32349995	7	76	theme	Bisecting	942:950	arg1	GlcNAc					952:957	Bisecting GlcNAc	942:957	Bisecting GlcNAc in fucosylated and fucosylated disialylated structures and monosialylation of fucosylated digalactosylated structures	942:1075	Bisecting GlcNAc in fucosylated and fucosylated disialylated structures and monosialylation of fucosylated digalactosylated structures were associated with a faster decrease of eGFR.
32349995	4	77	theme	DiaGene	498:504	arg1	study					547:551	an all-lines-of-care case-control study	513:551	an all-lines-of-care case-control study (n=1886)	513:560	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	77	theme	DiaGene	498:504	arg1	study					506:510	the DiaGene study	494:510	the DiaGene study	494:510	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	7	78	theme	fucosylated	978:988	arg1	structures					1003:1012	fucosylated and fucosylated disialylated structures	962:1012	structures	1003:1012	Bisecting GlcNAc in fucosylated and fucosylated disialylated structures and monosialylation of fucosylated digalactosylated structures were associated with a faster decrease of eGFR.
32349995	4	79	theme	glomerular	671:680	arg1	rate					693:696	estimated glomerular filtration rate	661:696	estimated glomerular filtration rate (eGFR)	661:703	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	4	79	theme	glomerular	671:680	arg1	eGFR					699:702	eGFR	699:702	eGFR	699:702	RESEARCH DESIGN AND METHODS In the DiaGene study, an all-lines-of-care case-control study (n=1886) with mean prospective follow-up of 7.0 years, the association between 58 IgG N-glycan profiles and estimated glomerular filtration rate (eGFR) and albumin-to-creatinine ratio (ACR) per year and during total follow-up was analyzed.
32349995	8	80	theme	eGFR	1208:1211	arg1	decline					1213:1219	less eGFR decline	1203:1219	less eGFR decline per year	1203:1228	Fucosylation of neutral and monogalactosylated structures was associated with less eGFR decline per year.
32676953	0	0	theme	Fold	93:96	arg1	Healing					104:110	Vocal Fold Wound Healing	87:110	Vocal Fold Wound Healing	87:110	Hyaluronic Acid/Alginate Hydrogel Containing Hepatocyte Growth Factor and Promotion of Vocal Fold Wound Healing.
32676953	4	1	theme	direct	623:628	arg1	injury					630:635	direct injury	623:635	direct injury	623:635	In addition, HGF-HA/ALG was injected into the VFs of rabbits immediately after direct injury and HGF or PBS was injected in the same manner into control groups.
32676953	11	2	theme	containing	1536:1545	arg1	hydrogel					1554:1561	CONCLUSION HGF containing HA/ALG hydrogel	1521:1561	CONCLUSION HGF containing HA/ALG hydrogel	1521:1561	CONCLUSION HGF containing HA/ALG hydrogel enhanced healing in our rabbit model of VF injury.
32676953	0	3	theme	Vocal	87:91	arg1	Healing					104:110	Vocal Fold Wound Healing	87:110	Vocal Fold Wound Healing	87:110	Hyaluronic Acid/Alginate Hydrogel Containing Hepatocyte Growth Factor and Promotion of Vocal Fold Wound Healing.
32676953	9	4	theme	viscoelastic	1163:1174	arg1	properties					1176:1185	viscoelastic properties	1163:1185	viscoelastic properties	1163:1185	Rabbits treated with HGF-HA/ALG showed improved mucosal vibrations and VF viscoelastic properties as compared with the PBS and HGF controls.
32676953	3	5	theme	HA/ALG	457:462	arg1	HGF-HA/ALG					484:493	HGF-HA/ALG	484:493	HGF-HA/ALG	484:493	METHODS HGF was loaded into HA/ALG composite hydrogel (HGF-HA/ALG) and its in vitro release profile was evaluated.
32676953	3	5	theme	HA/ALG	457:462	arg1	hydrogel					474:481	HA/ALG composite hydrogel	457:481	HA/ALG composite hydrogel (HGF-HA/ALG)	457:494	METHODS HGF was loaded into HA/ALG composite hydrogel (HGF-HA/ALG) and its in vitro release profile was evaluated.
32676953	0	6	theme	Healing	104:110	arg1	Promotion					74:82	Promotion	74:82	Promotion of Vocal Fold Wound Healing	74:110	Hyaluronic Acid/Alginate Hydrogel Containing Hepatocyte Growth Factor and Promotion of Vocal Fold Wound Healing.
32676953	0	6	theme	Healing	104:110	arg1	Factor					63:68	Hepatocyte Growth Factor	45:68	Hepatocyte Growth Factor	45:68	Hyaluronic Acid/Alginate Hydrogel Containing Hepatocyte Growth Factor and Promotion of Vocal Fold Wound Healing.
32676953	2	7	from	injury	403:408	arg1	model					422:426	a rabbit model	413:426	a rabbit model	413:426	This study was undertaken to determine whether the therapeutic efficacy of HGF could be enhanced by applying it in hyaluronic acid and alginate (HA/ALG) composite hydrogels into VFs after injury in a rabbit model.
32676953	8	8	theme	HGF	1028:1030	arg1	release					1032:1038	HGF release	1028:1038	HGF release from HGF-HA/ALG	1028:1054	RESULTS HGF release from HGF-HA/ALG was sustained for up to 3 weeks.
32676953	5	9	located	observed	731:738	arg1	3 months					756:763	3 months post-injury	756:775	3 months post-injury	756:775	Macroscopic features were observed by endoscopy at 3 months post-injury.
32676953	5	9	located	observed	731:738	arg2	features					717:724	Macroscopic features	705:724	Macroscopic features	705:724	Macroscopic features were observed by endoscopy at 3 months post-injury.
32676953	2	10	theme	therapeutic	266:276	arg1	efficacy					278:285	the therapeutic efficacy	262:285	the therapeutic efficacy of HGF	262:292	This study was undertaken to determine whether the therapeutic efficacy of HGF could be enhanced by applying it in hyaluronic acid and alginate (HA/ALG) composite hydrogels into VFs after injury in a rabbit model.
32676953	3	11	theme	in	504:505	arg1	profile					521:527	its in vitro release profile	500:527	its in vitro release profile	500:527	METHODS HGF was loaded into HA/ALG composite hydrogel (HGF-HA/ALG) and its in vitro release profile was evaluated.
32676953	0	12	theme	Wound	98:102	arg1	Healing					104:110	Vocal Fold Wound Healing	87:110	Vocal Fold Wound Healing	87:110	Hyaluronic Acid/Alginate Hydrogel Containing Hepatocyte Growth Factor and Promotion of Vocal Fold Wound Healing.
32676953	6	13	theme	viscoelastic	833:844	arg1	properties					846:855	viscoelastic properties	833:855	viscoelastic properties	833:855	Functional analyses including mucosal waves of VFs and viscoelastic properties were performed by kymography following high-speed digital imaging and rheometer.
32676953	10	14	theme	HGF-HA/ALG	1440:1449	arg1	animals					1459:1465	HGF-HA/ALG treated animals	1440:1465	HGF-HA/ALG treated animals	1440:1465	Histopathological staining revealed HGF-HA/ALG treated VFs showed less fibrosis than PBS and HGF controls, and immunohistochemical analysis demonstrated amounts of type I collagen and fibronectin were lower in HGF-HA/ALG treated animals than in PBS and HGF controls at 3 months post-injury.
32676953	11	15	theme	HA/ALG	1547:1552	arg1	hydrogel					1554:1561	CONCLUSION HGF containing HA/ALG hydrogel	1521:1561	CONCLUSION HGF containing HA/ALG hydrogel	1521:1561	CONCLUSION HGF containing HA/ALG hydrogel enhanced healing in our rabbit model of VF injury.
32676953	10	16	dep	demonstrated	1370:1381	arg1	lower					1431:1435	lower	1431:1435	lower	1431:1435	Histopathological staining revealed HGF-HA/ALG treated VFs showed less fibrosis than PBS and HGF controls, and immunohistochemical analysis demonstrated amounts of type I collagen and fibronectin were lower in HGF-HA/ALG treated animals than in PBS and HGF controls at 3 months post-injury.
32676953	8	17	dep	RESULTS	1020:1026	arg1	sustained					1060:1068	sustained	1060:1068	was sustained for up to 3 weeks	1056:1086	RESULTS HGF release from HGF-HA/ALG was sustained for up to 3 weeks.
32676953	10	18	theme	treated	1451:1457	arg1	animals					1459:1465	HGF-HA/ALG treated animals	1440:1465	HGF-HA/ALG treated animals	1440:1465	Histopathological staining revealed HGF-HA/ALG treated VFs showed less fibrosis than PBS and HGF controls, and immunohistochemical analysis demonstrated amounts of type I collagen and fibronectin were lower in HGF-HA/ALG treated animals than in PBS and HGF controls at 3 months post-injury.
32676953	10	19	theme	HGF	1483:1485	arg1	controls					1487:1494	HGF controls	1483:1494	HGF controls	1483:1494	Histopathological staining revealed HGF-HA/ALG treated VFs showed less fibrosis than PBS and HGF controls, and immunohistochemical analysis demonstrated amounts of type I collagen and fibronectin were lower in HGF-HA/ALG treated animals than in PBS and HGF controls at 3 months post-injury.
32676953	5	20	theme	post-injury	765:775	arg1	3 months					756:763	3 months post-injury	756:775	3 months post-injury	756:775	Macroscopic features were observed by endoscopy at 3 months post-injury.
32676953	10	21	theme	less	1296:1299	arg1	fibrosis					1301:1308	less fibrosis	1296:1308	less fibrosis	1296:1308	Histopathological staining revealed HGF-HA/ALG treated VFs showed less fibrosis than PBS and HGF controls, and immunohistochemical analysis demonstrated amounts of type I collagen and fibronectin were lower in HGF-HA/ALG treated animals than in PBS and HGF controls at 3 months post-injury.
32676953	6	22	theme	VFs	825:827	arg1	waves					816:820	mucosal waves	808:820	mucosal waves of VFs and viscoelastic properties	808:855	Functional analyses including mucosal waves of VFs and viscoelastic properties were performed by kymography following high-speed digital imaging and rheometer.
32676953	11	23	theme	rabbit	1587:1592	arg1	model					1594:1598	our rabbit model	1583:1598	our rabbit model of VF injury	1583:1611	CONCLUSION HGF containing HA/ALG hydrogel enhanced healing in our rabbit model of VF injury.
32676953	4	24	theme	control	689:695	arg1	groups					697:702	control groups	689:702	control groups	689:702	In addition, HGF-HA/ALG was injected into the VFs of rabbits immediately after direct injury and HGF or PBS was injected in the same manner into control groups.
32676953	0	25	theme	Acid/Alginate	11:23	arg1	Hydrogel					25:32	Hyaluronic Acid/Alginate Hydrogel	0:32	Hyaluronic Acid/Alginate Hydrogel	0:32	Hyaluronic Acid/Alginate Hydrogel Containing Hepatocyte Growth Factor and Promotion of Vocal Fold Wound Healing.
32676953	2	26	theme	alginate	350:357	arg1	hydrogels					378:386	hyaluronic acid and alginate (HA/ALG) composite hydrogels	330:386	hyaluronic acid and alginate (HA/ALG) composite hydrogels into VFs after injury in a rabbit model	330:426	This study was undertaken to determine whether the therapeutic efficacy of HGF could be enhanced by applying it in hyaluronic acid and alginate (HA/ALG) composite hydrogels into VFs after injury in a rabbit model.
32676953	0	27	theme	Hyaluronic	0:9	arg1	Hydrogel					25:32	Hyaluronic Acid/Alginate Hydrogel	0:32	Hyaluronic Acid/Alginate Hydrogel	0:32	Hyaluronic Acid/Alginate Hydrogel Containing Hepatocyte Growth Factor and Promotion of Vocal Fold Wound Healing.
32676953	9	28	theme	PBS	1208:1210	arg1	controls					1220:1227	the PBS and HGF controls	1204:1227	controls	1220:1227	Rabbits treated with HGF-HA/ALG showed improved mucosal vibrations and VF viscoelastic properties as compared with the PBS and HGF controls.
32676953	2	29	theme	HGF	290:292	arg1	efficacy					278:285	the therapeutic efficacy	262:285	the therapeutic efficacy of HGF	262:292	This study was undertaken to determine whether the therapeutic efficacy of HGF could be enhanced by applying it in hyaluronic acid and alginate (HA/ALG) composite hydrogels into VFs after injury in a rabbit model.
32676953	10	30	theme	HGF	1323:1325	arg1	controls					1327:1334	HGF controls	1323:1334	HGF controls	1323:1334	Histopathological staining revealed HGF-HA/ALG treated VFs showed less fibrosis than PBS and HGF controls, and immunohistochemical analysis demonstrated amounts of type I collagen and fibronectin were lower in HGF-HA/ALG treated animals than in PBS and HGF controls at 3 months post-injury.
32676953	1	31	theme	vocal	184:188	arg1	healing					206:212	vocal fold (VF) wound healing	184:212	vocal fold (VF) wound healing	184:212	BACKGROUND Hepatocyte growth factor (HGF) has been shown to facilitate vocal fold (VF) wound healing.
32676953	9	32	theme	mucosal	1137:1143	arg1	vibrations					1145:1154	improved mucosal vibrations	1128:1154	improved mucosal vibrations	1128:1154	Rabbits treated with HGF-HA/ALG showed improved mucosal vibrations and VF viscoelastic properties as compared with the PBS and HGF controls.
32676953	10	33	theme	post-injury	1508:1518	arg1	3 months					1499:1506	3 months post-injury	1499:1518	3 months post-injury	1499:1518	Histopathological staining revealed HGF-HA/ALG treated VFs showed less fibrosis than PBS and HGF controls, and immunohistochemical analysis demonstrated amounts of type I collagen and fibronectin were lower in HGF-HA/ALG treated animals than in PBS and HGF controls at 3 months post-injury.
32676953	10	34	theme	type	1394:1397	arg1	collagen					1401:1408	type I collagen	1394:1408	type I collagen	1394:1408	Histopathological staining revealed HGF-HA/ALG treated VFs showed less fibrosis than PBS and HGF controls, and immunohistochemical analysis demonstrated amounts of type I collagen and fibronectin were lower in HGF-HA/ALG treated animals than in PBS and HGF controls at 3 months post-injury.
32676953	4	35	theme	same	672:675	arg1	manner					677:682	the same manner	668:682	the same manner	668:682	In addition, HGF-HA/ALG was injected into the VFs of rabbits immediately after direct injury and HGF or PBS was injected in the same manner into control groups.
32676953	1	36	theme	fold	190:193	arg1	healing					206:212	vocal fold (VF) wound healing	184:212	vocal fold (VF) wound healing	184:212	BACKGROUND Hepatocyte growth factor (HGF) has been shown to facilitate vocal fold (VF) wound healing.
32676953	3	37	theme	release	513:519	arg1	profile					521:527	its in vitro release profile	500:527	its in vitro release profile	500:527	METHODS HGF was loaded into HA/ALG composite hydrogel (HGF-HA/ALG) and its in vitro release profile was evaluated.
32676953	9	38	theme	HGF	1216:1218	arg1	controls					1220:1227	the PBS and HGF controls	1204:1227	controls	1220:1227	Rabbits treated with HGF-HA/ALG showed improved mucosal vibrations and VF viscoelastic properties as compared with the PBS and HGF controls.
32676953	1	39	theme	BACKGROUND	113:122	arg1	factor					142:147	BACKGROUND Hepatocyte growth factor	113:147	BACKGROUND Hepatocyte growth factor (HGF)	113:153	BACKGROUND Hepatocyte growth factor (HGF) has been shown to facilitate vocal fold (VF) wound healing.
32676953	1	39	theme	BACKGROUND	113:122	arg1	HGF					150:152	HGF	150:152	HGF	150:152	BACKGROUND Hepatocyte growth factor (HGF) has been shown to facilitate vocal fold (VF) wound healing.
32676953	3	40	theme	composite	464:472	arg1	HGF-HA/ALG					484:493	HGF-HA/ALG	484:493	HGF-HA/ALG	484:493	METHODS HGF was loaded into HA/ALG composite hydrogel (HGF-HA/ALG) and its in vitro release profile was evaluated.
32676953	3	40	theme	composite	464:472	arg1	hydrogel					474:481	HA/ALG composite hydrogel	457:481	HA/ALG composite hydrogel (HGF-HA/ALG)	457:494	METHODS HGF was loaded into HA/ALG composite hydrogel (HGF-HA/ALG) and its in vitro release profile was evaluated.
32676953	0	41	theme	Growth	56:61	arg1	Factor					63:68	Hepatocyte Growth Factor	45:68	Hepatocyte Growth Factor	45:68	Hyaluronic Acid/Alginate Hydrogel Containing Hepatocyte Growth Factor and Promotion of Vocal Fold Wound Healing.
32676953	1	42	theme	VF	196:197	arg1	healing					206:212	vocal fold (VF) wound healing	184:212	vocal fold (VF) wound healing	184:212	BACKGROUND Hepatocyte growth factor (HGF) has been shown to facilitate vocal fold (VF) wound healing.
32676953	4	43	theme	rabbits	597:603	arg1	VFs					590:592	the VFs	586:592	the VFs of rabbits	586:603	In addition, HGF-HA/ALG was injected into the VFs of rabbits immediately after direct injury and HGF or PBS was injected in the same manner into control groups.
32676953	2	44	theme	composite	368:376	arg1	hydrogels					378:386	hyaluronic acid and alginate (HA/ALG) composite hydrogels	330:386	hyaluronic acid and alginate (HA/ALG) composite hydrogels into VFs after injury in a rabbit model	330:426	This study was undertaken to determine whether the therapeutic efficacy of HGF could be enhanced by applying it in hyaluronic acid and alginate (HA/ALG) composite hydrogels into VFs after injury in a rabbit model.
32676953	0	45	theme	Hepatocyte	45:54	arg1	Factor					63:68	Hepatocyte Growth Factor	45:68	Hepatocyte Growth Factor	45:68	Hyaluronic Acid/Alginate Hydrogel Containing Hepatocyte Growth Factor and Promotion of Vocal Fold Wound Healing.
32676953	6	46	theme	digital	907:913	arg1	imaging					915:921	high-speed digital imaging	896:921	high-speed digital imaging	896:921	Functional analyses including mucosal waves of VFs and viscoelastic properties were performed by kymography following high-speed digital imaging and rheometer.
32676953	5	47	theme	Macroscopic	705:715	arg1	features					717:724	Macroscopic features	705:724	Macroscopic features	705:724	Macroscopic features were observed by endoscopy at 3 months post-injury.
32676953	10	48	theme	immunohistochemical	1341:1359	arg1	analysis					1361:1368	immunohistochemical analysis	1341:1368	immunohistochemical analysis	1341:1368	Histopathological staining revealed HGF-HA/ALG treated VFs showed less fibrosis than PBS and HGF controls, and immunohistochemical analysis demonstrated amounts of type I collagen and fibronectin were lower in HGF-HA/ALG treated animals than in PBS and HGF controls at 3 months post-injury.
32676953	2	49	theme	rabbit	415:420	arg1	model					422:426	a rabbit model	413:426	a rabbit model	413:426	This study was undertaken to determine whether the therapeutic efficacy of HGF could be enhanced by applying it in hyaluronic acid and alginate (HA/ALG) composite hydrogels into VFs after injury in a rabbit model.
32676953	6	50	theme	high-speed	896:905	arg1	imaging					915:921	high-speed digital imaging	896:921	high-speed digital imaging	896:921	Functional analyses including mucosal waves of VFs and viscoelastic properties were performed by kymography following high-speed digital imaging and rheometer.
32676953	1	51	theme	Hepatocyte	124:133	arg1	factor					142:147	BACKGROUND Hepatocyte growth factor	113:147	BACKGROUND Hepatocyte growth factor (HGF)	113:153	BACKGROUND Hepatocyte growth factor (HGF) has been shown to facilitate vocal fold (VF) wound healing.
32676953	1	51	theme	Hepatocyte	124:133	arg1	HGF					150:152	HGF	150:152	HGF	150:152	BACKGROUND Hepatocyte growth factor (HGF) has been shown to facilitate vocal fold (VF) wound healing.
32676953	6	52	theme	properties	846:855	arg1	waves					816:820	mucosal waves	808:820	mucosal waves of VFs and viscoelastic properties	808:855	Functional analyses including mucosal waves of VFs and viscoelastic properties were performed by kymography following high-speed digital imaging and rheometer.
32676953	10	53	theme	collagen	1401:1408	arg1	collagen					1401:1408	type I collagen	1394:1408	type I collagen	1394:1408	Histopathological staining revealed HGF-HA/ALG treated VFs showed less fibrosis than PBS and HGF controls, and immunohistochemical analysis demonstrated amounts of type I collagen and fibronectin were lower in HGF-HA/ALG treated animals than in PBS and HGF controls at 3 months post-injury.
32676953	10	53	theme	collagen	1401:1408	arg1	amounts					1383:1389	amounts	1383:1389	amounts of type I collagen and fibronectin	1383:1424	Histopathological staining revealed HGF-HA/ALG treated VFs showed less fibrosis than PBS and HGF controls, and immunohistochemical analysis demonstrated amounts of type I collagen and fibronectin were lower in HGF-HA/ALG treated animals than in PBS and HGF controls at 3 months post-injury.
32676953	10	53	theme	collagen	1401:1408	arg1	fibronectin					1414:1424	fibronectin	1414:1424	fibronectin	1414:1424	Histopathological staining revealed HGF-HA/ALG treated VFs showed less fibrosis than PBS and HGF controls, and immunohistochemical analysis demonstrated amounts of type I collagen and fibronectin were lower in HGF-HA/ALG treated animals than in PBS and HGF controls at 3 months post-injury.
32676953	6	54	theme	Functional	778:787	arg1	analyses					789:796	Functional analyses	778:796	Functional analyses including mucosal waves of VFs and viscoelastic properties	778:855	Functional analyses including mucosal waves of VFs and viscoelastic properties were performed by kymography following high-speed digital imaging and rheometer.
32676953	6	54	theme	Functional	778:787	arg1	waves					816:820	mucosal waves	808:820	mucosal waves of VFs and viscoelastic properties	808:855	Functional analyses including mucosal waves of VFs and viscoelastic properties were performed by kymography following high-speed digital imaging and rheometer.
32676953	11	55	theme	injury	1606:1611	arg1	model					1594:1598	our rabbit model	1583:1598	our rabbit model of VF injury	1583:1611	CONCLUSION HGF containing HA/ALG hydrogel enhanced healing in our rabbit model of VF injury.
32676953	1	56	theme	growth	135:140	arg1	factor					142:147	BACKGROUND Hepatocyte growth factor	113:147	BACKGROUND Hepatocyte growth factor (HGF)	113:153	BACKGROUND Hepatocyte growth factor (HGF) has been shown to facilitate vocal fold (VF) wound healing.
32676953	1	56	theme	growth	135:140	arg1	HGF					150:152	HGF	150:152	HGF	150:152	BACKGROUND Hepatocyte growth factor (HGF) has been shown to facilitate vocal fold (VF) wound healing.
32676953	10	57	theme	Histopathological	1230:1246	arg1	staining					1248:1255	Histopathological staining	1230:1255	Histopathological staining	1230:1255	Histopathological staining revealed HGF-HA/ALG treated VFs showed less fibrosis than PBS and HGF controls, and immunohistochemical analysis demonstrated amounts of type I collagen and fibronectin were lower in HGF-HA/ALG treated animals than in PBS and HGF controls at 3 months post-injury.
32676953	10	58	theme	fibronectin	1414:1424	arg1	collagen					1401:1408	type I collagen	1394:1408	type I collagen	1394:1408	Histopathological staining revealed HGF-HA/ALG treated VFs showed less fibrosis than PBS and HGF controls, and immunohistochemical analysis demonstrated amounts of type I collagen and fibronectin were lower in HGF-HA/ALG treated animals than in PBS and HGF controls at 3 months post-injury.
32676953	10	58	theme	fibronectin	1414:1424	arg1	amounts					1383:1389	amounts	1383:1389	amounts of type I collagen and fibronectin	1383:1424	Histopathological staining revealed HGF-HA/ALG treated VFs showed less fibrosis than PBS and HGF controls, and immunohistochemical analysis demonstrated amounts of type I collagen and fibronectin were lower in HGF-HA/ALG treated animals than in PBS and HGF controls at 3 months post-injury.
32676953	10	58	theme	fibronectin	1414:1424	arg1	fibronectin					1414:1424	fibronectin	1414:1424	fibronectin	1414:1424	Histopathological staining revealed HGF-HA/ALG treated VFs showed less fibrosis than PBS and HGF controls, and immunohistochemical analysis demonstrated amounts of type I collagen and fibronectin were lower in HGF-HA/ALG treated animals than in PBS and HGF controls at 3 months post-injury.
32676953	9	59	theme	improved	1128:1135	arg1	vibrations					1145:1154	improved mucosal vibrations	1128:1154	improved mucosal vibrations	1128:1154	Rabbits treated with HGF-HA/ALG showed improved mucosal vibrations and VF viscoelastic properties as compared with the PBS and HGF controls.
32676953	1	60	theme	wound	200:204	arg1	healing					206:212	vocal fold (VF) wound healing	184:212	vocal fold (VF) wound healing	184:212	BACKGROUND Hepatocyte growth factor (HGF) has been shown to facilitate vocal fold (VF) wound healing.
32676953	11	61	theme	VF	1603:1604	arg1	injury					1606:1611	VF injury	1603:1611	VF injury	1603:1611	CONCLUSION HGF containing HA/ALG hydrogel enhanced healing in our rabbit model of VF injury.
32676953	3	62	dep	METHODS	429:435	arg1	loaded					445:450	loaded	445:450	was loaded into HA/ALG composite hydrogel (HGF-HA/ALG) and its in vitro release profile	441:527	METHODS HGF was loaded into HA/ALG composite hydrogel (HGF-HA/ALG) and its in vitro release profile was evaluated.
32676953	7	63	theme	immunohistochemical	960:978	arg1	evaluations					980:990	Histopathological and immunohistochemical evaluations	938:990	Histopathological and immunohistochemical evaluations	938:990	Histopathological and immunohistochemical evaluations were also conducted on VFs.
32676953	3	64	dep	in	504:505	arg1	vitro					507:511	vitro	507:511	vitro	507:511	METHODS HGF was loaded into HA/ALG composite hydrogel (HGF-HA/ALG) and its in vitro release profile was evaluated.
32676953	6	65	theme	mucosal	808:814	arg1	waves					816:820	mucosal waves	808:820	mucosal waves of VFs and viscoelastic properties	808:855	Functional analyses including mucosal waves of VFs and viscoelastic properties were performed by kymography following high-speed digital imaging and rheometer.
32676953	11	66	theme	CONCLUSION	1521:1530	arg1	hydrogel					1554:1561	CONCLUSION HGF containing HA/ALG hydrogel	1521:1561	CONCLUSION HGF containing HA/ALG hydrogel	1521:1561	CONCLUSION HGF containing HA/ALG hydrogel enhanced healing in our rabbit model of VF injury.
32676953	11	67	theme	HGF	1532:1534	arg1	hydrogel					1554:1561	CONCLUSION HGF containing HA/ALG hydrogel	1521:1561	CONCLUSION HGF containing HA/ALG hydrogel	1521:1561	CONCLUSION HGF containing HA/ALG hydrogel enhanced healing in our rabbit model of VF injury.
32676953	2	68	theme	acid	341:344	arg1	hydrogels					378:386	hyaluronic acid and alginate (HA/ALG) composite hydrogels	330:386	hyaluronic acid and alginate (HA/ALG) composite hydrogels into VFs after injury in a rabbit model	330:426	This study was undertaken to determine whether the therapeutic efficacy of HGF could be enhanced by applying it in hyaluronic acid and alginate (HA/ALG) composite hydrogels into VFs after injury in a rabbit model.
32676953	7	69	theme	Histopathological	938:954	arg1	evaluations					980:990	Histopathological and immunohistochemical evaluations	938:990	Histopathological and immunohistochemical evaluations	938:990	Histopathological and immunohistochemical evaluations were also conducted on VFs.
32676953	2	70	theme	hyaluronic	330:339	arg1	HA/ALG					360:365	HA/ALG	360:365	HA/ALG	360:365	This study was undertaken to determine whether the therapeutic efficacy of HGF could be enhanced by applying it in hyaluronic acid and alginate (HA/ALG) composite hydrogels into VFs after injury in a rabbit model.
32676953	2	70	theme	hyaluronic	330:339	arg1	acid					341:344	hyaluronic acid	330:344	hyaluronic acid	330:344	This study was undertaken to determine whether the therapeutic efficacy of HGF could be enhanced by applying it in hyaluronic acid and alginate (HA/ALG) composite hydrogels into VFs after injury in a rabbit model.
32676953	10	71	theme	treated	1277:1283	arg1	VFs					1285:1287	treated VFs	1277:1287	treated VFs	1277:1287	Histopathological staining revealed HGF-HA/ALG treated VFs showed less fibrosis than PBS and HGF controls, and immunohistochemical analysis demonstrated amounts of type I collagen and fibronectin were lower in HGF-HA/ALG treated animals than in PBS and HGF controls at 3 months post-injury.
32676953	8	72	from	HGF-HA/ALG	1045:1054	arg1	release					1032:1038	HGF release	1028:1038	HGF release from HGF-HA/ALG	1028:1054	RESULTS HGF release from HGF-HA/ALG was sustained for up to 3 weeks.
34896953	4	0	theme	larger	524:529	arg1	emulsification					576:589	emulsification	576:589	emulsification	576:589	Results showed that emulsion gel containing cellulose nanofibers has slightly larger droplet diameter, better viscoelasticity and emulsification.
34896953	4	0	theme	larger	524:529	arg1	diameter					539:546	slightly larger droplet diameter	515:546	slightly larger droplet diameter	515:546	Results showed that emulsion gel containing cellulose nanofibers has slightly larger droplet diameter, better viscoelasticity and emulsification.
34896953	4	0	theme	larger	524:529	arg1	viscoelasticity					556:570	better viscoelasticity	549:570	better viscoelasticity	549:570	Results showed that emulsion gel containing cellulose nanofibers has slightly larger droplet diameter, better viscoelasticity and emulsification.
34896953	5	1	theme	thinner	635:641	arg1	wall					648:651	thinner pore wall	635:651	thinner pore wall	635:651	Composite aerogel has larger pore size and thinner pore wall.
34896953	2	2	theme	embedding	346:354	arg1	formation					335:343	aerogel formation	327:343	aerogel formation	327:343	Pickering emulsion was a template for aerogel formation, embedding thymol.
34896953	2	2	theme	embedding	346:354	arg1	thymol					356:361	embedding thymol	346:361	embedding thymol	346:361	Pickering emulsion was a template for aerogel formation, embedding thymol.
34896953	1	3	theme	enhanced	244:251	arg1	properties					264:273	enhanced structural properties	244:273	enhanced structural properties	244:273	Citrus pectin was used as a precursor and cellulose nanofibers as a reinforcing agent, a mixed aerogel with enhanced structural properties was prepared.
34896953	5	4	theme	pore	643:646	arg1	wall					648:651	thinner pore wall	635:651	thinner pore wall	635:651	Composite aerogel has larger pore size and thinner pore wall.
34896953	2	5	theme	Pickering	289:297	arg1	emulsion					299:306	Pickering emulsion	289:306	Pickering emulsion	289:306	Pickering emulsion was a template for aerogel formation, embedding thymol.
34896953	2	5	theme	Pickering	289:297	arg1	template					314:321	a template	312:321	a template for aerogel formation, embedding thymol	312:361	Pickering emulsion was a template for aerogel formation, embedding thymol.
34896953	10	6	from	humidity	982:989	arg1	package					994:1000	package	994:1000	package	994:1000	It was found that relative humidity in package can be stabilized at about 97%.
34896953	1	7	theme	structural	253:262	arg1	properties					264:273	enhanced structural properties	244:273	enhanced structural properties	244:273	Citrus pectin was used as a precursor and cellulose nanofibers as a reinforcing agent, a mixed aerogel with enhanced structural properties was prepared.
34896953	0	8	theme	controlled	98:107	arg1	packaging					109:117	controlled packaging	98:117	controlled packaging of edible fungi	98:133	Facile fabrication of multifunctional citrus pectin aerogel fortified with cellulose nanofiber as controlled packaging of edible fungi.
34896953	0	9	theme	edible	122:127	arg1	fungi					129:133	edible fungi	122:133	edible fungi	122:133	Facile fabrication of multifunctional citrus pectin aerogel fortified with cellulose nanofiber as controlled packaging of edible fungi.
34896953	3	10	from	application	378:388	arg1	packaging					413:421	humidity regulating packaging	393:421	humidity regulating packaging	393:421	Its potential application in humidity regulating packaging has been investigated.
34896953	1	11	used	used	154:157	arg2	agent					216:220	a reinforcing agent	202:220	a reinforcing agent	202:220	Citrus pectin was used as a precursor and cellulose nanofibers as a reinforcing agent, a mixed aerogel with enhanced structural properties was prepared.
34896953	1	11	used	used	154:157	arg2	precursor					164:172	a precursor	162:172	a precursor	162:172	Citrus pectin was used as a precursor and cellulose nanofibers as a reinforcing agent, a mixed aerogel with enhanced structural properties was prepared.
34896953	1	11	used	used	154:157	arg2	pectin					143:148	Citrus pectin	136:148	Citrus pectin	136:148	Citrus pectin was used as a precursor and cellulose nanofibers as a reinforcing agent, a mixed aerogel with enhanced structural properties was prepared.
34896953	1	11	used	used	154:157	arg2	nanofibers					188:197	cellulose nanofibers	178:197	cellulose nanofibers	178:197	Citrus pectin was used as a precursor and cellulose nanofibers as a reinforcing agent, a mixed aerogel with enhanced structural properties was prepared.
34896953	11	12	theme	membrane	1078:1085	arg1	integrity					1087:1095	cell membrane integrity	1073:1095	cell membrane integrity	1073:1095	Hardness, color, total phenol content, cell membrane integrity and total antioxidant capacity of Agaricus bisporus were maintained and fresh-keeping period was extended to 5 days.
34896953	5	13	theme	larger	614:619	arg1	size					626:629	larger pore size	614:629	larger pore size	614:629	Composite aerogel has larger pore size and thinner pore wall.
34896953	0	14	theme	Facile	0:5	arg1	fabrication					7:17	Facile fabrication	0:17	Facile fabrication of multifunctional citrus pectin aerogel fortified with cellulose	0:83	Facile fabrication of multifunctional citrus pectin aerogel fortified with cellulose nanofiber as controlled packaging of edible fungi.
34896953	5	15	theme	pore	621:624	arg1	size					626:629	larger pore size	614:629	larger pore size	614:629	Composite aerogel has larger pore size and thinner pore wall.
34896953	4	16	theme	cellulose	490:498	arg1	nanofibers					500:509	emulsion gel containing cellulose nanofibers	466:509	emulsion gel containing cellulose nanofibers	466:509	Results showed that emulsion gel containing cellulose nanofibers has slightly larger droplet diameter, better viscoelasticity and emulsification.
34896953	1	17	theme	cellulose	178:186	arg1	nanofibers					188:197	cellulose nanofibers	178:197	cellulose nanofibers	178:197	Citrus pectin was used as a precursor and cellulose nanofibers as a reinforcing agent, a mixed aerogel with enhanced structural properties was prepared.
34896953	1	17	theme	cellulose	178:186	arg1	pectin					143:148	Citrus pectin	136:148	Citrus pectin	136:148	Citrus pectin was used as a precursor and cellulose nanofibers as a reinforcing agent, a mixed aerogel with enhanced structural properties was prepared.
34896953	3	18	theme	humidity	393:400	arg1	packaging					413:421	humidity regulating packaging	393:421	humidity regulating packaging	393:421	Its potential application in humidity regulating packaging has been investigated.
34896953	11	19	theme	cell	1073:1076	arg1	integrity					1087:1095	cell membrane integrity	1073:1095	cell membrane integrity	1073:1095	Hardness, color, total phenol content, cell membrane integrity and total antioxidant capacity of Agaricus bisporus were maintained and fresh-keeping period was extended to 5 days.
34896953	3	20	theme	potential	368:376	arg1	application					378:388	Its potential application	364:388	Its potential application in humidity regulating packaging	364:421	Its potential application in humidity regulating packaging has been investigated.
34896953	10	21	theme	relative	973:980	arg1	humidity					982:989	relative humidity	973:989	relative humidity in package	973:1000	It was found that relative humidity in package can be stabilized at about 97%.
34896953	11	22	theme	antioxidant	1107:1117	arg1	capacity					1119:1126	total antioxidant capacity	1101:1126	total antioxidant capacity	1101:1126	Hardness, color, total phenol content, cell membrane integrity and total antioxidant capacity of Agaricus bisporus were maintained and fresh-keeping period was extended to 5 days.
34896953	0	23	theme	fungi	129:133	arg1	packaging					109:117	controlled packaging	98:117	controlled packaging of edible fungi	98:133	Facile fabrication of multifunctional citrus pectin aerogel fortified with cellulose nanofiber as controlled packaging of edible fungi.
34896953	4	24	theme	emulsion	466:473	arg1	nanofibers					500:509	emulsion gel containing cellulose nanofibers	466:509	emulsion gel containing cellulose nanofibers	466:509	Results showed that emulsion gel containing cellulose nanofibers has slightly larger droplet diameter, better viscoelasticity and emulsification.
34896953	11	25	theme	total	1051:1055	arg1	content					1064:1070	total phenol content	1051:1070	total phenol content	1051:1070	Hardness, color, total phenol content, cell membrane integrity and total antioxidant capacity of Agaricus bisporus were maintained and fresh-keeping period was extended to 5 days.
34896953	0	26	theme	pectin	45:50	arg1	aerogel					52:58	multifunctional citrus pectin aerogel	22:58	multifunctional citrus pectin aerogel fortified with cellulose	22:83	Facile fabrication of multifunctional citrus pectin aerogel fortified with cellulose nanofiber as controlled packaging of edible fungi.
34896953	11	27	theme	bisporus	1140:1147	arg1	content					1064:1070	total phenol content	1051:1070	total phenol content	1051:1070	Hardness, color, total phenol content, cell membrane integrity and total antioxidant capacity of Agaricus bisporus were maintained and fresh-keeping period was extended to 5 days.
34896953	11	27	theme	bisporus	1140:1147	arg1	integrity					1087:1095	cell membrane integrity	1073:1095	cell membrane integrity	1073:1095	Hardness, color, total phenol content, cell membrane integrity and total antioxidant capacity of Agaricus bisporus were maintained and fresh-keeping period was extended to 5 days.
34896953	11	27	theme	bisporus	1140:1147	arg1	Hardness					1034:1041	Hardness	1034:1041	Hardness	1034:1041	Hardness, color, total phenol content, cell membrane integrity and total antioxidant capacity of Agaricus bisporus were maintained and fresh-keeping period was extended to 5 days.
34896953	11	27	theme	bisporus	1140:1147	arg1	color					1044:1048	color	1044:1048	color	1044:1048	Hardness, color, total phenol content, cell membrane integrity and total antioxidant capacity of Agaricus bisporus were maintained and fresh-keeping period was extended to 5 days.
34896953	11	27	theme	bisporus	1140:1147	arg1	capacity					1119:1126	total antioxidant capacity	1101:1126	total antioxidant capacity	1101:1126	Hardness, color, total phenol content, cell membrane integrity and total antioxidant capacity of Agaricus bisporus were maintained and fresh-keeping period was extended to 5 days.
34896953	1	28	theme	reinforcing	204:214	arg1	pectin					143:148	Citrus pectin	136:148	Citrus pectin	136:148	Citrus pectin was used as a precursor and cellulose nanofibers as a reinforcing agent, a mixed aerogel with enhanced structural properties was prepared.
34896953	1	28	theme	reinforcing	204:214	arg1	agent					216:220	a reinforcing agent	202:220	a reinforcing agent	202:220	Citrus pectin was used as a precursor and cellulose nanofibers as a reinforcing agent, a mixed aerogel with enhanced structural properties was prepared.
34896953	0	29	theme	citrus	38:43	arg1	aerogel					52:58	multifunctional citrus pectin aerogel	22:58	multifunctional citrus pectin aerogel fortified with cellulose	22:83	Facile fabrication of multifunctional citrus pectin aerogel fortified with cellulose nanofiber as controlled packaging of edible fungi.
34896953	4	30	theme	containing	479:488	arg1	nanofibers					500:509	emulsion gel containing cellulose nanofibers	466:509	emulsion gel containing cellulose nanofibers	466:509	Results showed that emulsion gel containing cellulose nanofibers has slightly larger droplet diameter, better viscoelasticity and emulsification.
34896953	0	31	theme	multifunctional	22:36	arg1	aerogel					52:58	multifunctional citrus pectin aerogel	22:58	multifunctional citrus pectin aerogel fortified with cellulose	22:83	Facile fabrication of multifunctional citrus pectin aerogel fortified with cellulose nanofiber as controlled packaging of edible fungi.
34896953	11	32	theme	total	1101:1105	arg1	capacity					1119:1126	total antioxidant capacity	1101:1126	total antioxidant capacity	1101:1126	Hardness, color, total phenol content, cell membrane integrity and total antioxidant capacity of Agaricus bisporus were maintained and fresh-keeping period was extended to 5 days.
34896953	3	33	theme	regulating	402:411	arg1	packaging					413:421	humidity regulating packaging	393:421	humidity regulating packaging	393:421	Its potential application in humidity regulating packaging has been investigated.
34896953	4	34	theme	better	549:554	arg1	diameter					539:546	slightly larger droplet diameter	515:546	slightly larger droplet diameter	515:546	Results showed that emulsion gel containing cellulose nanofibers has slightly larger droplet diameter, better viscoelasticity and emulsification.
34896953	4	34	theme	better	549:554	arg1	viscoelasticity					556:570	better viscoelasticity	549:570	better viscoelasticity	549:570	Results showed that emulsion gel containing cellulose nanofibers has slightly larger droplet diameter, better viscoelasticity and emulsification.
34896953	4	35	theme	gel	475:477	arg1	nanofibers					500:509	emulsion gel containing cellulose nanofibers	466:509	emulsion gel containing cellulose nanofibers	466:509	Results showed that emulsion gel containing cellulose nanofibers has slightly larger droplet diameter, better viscoelasticity and emulsification.
34896953	5	36	theme	Composite	592:600	arg1	aerogel					602:608	Composite aerogel	592:608	Composite aerogel	592:608	Composite aerogel has larger pore size and thinner pore wall.
34896953	4	37	contain	has	511:513	arg2	diameter					539:546	slightly larger droplet diameter	515:546	slightly larger droplet diameter	515:546	Results showed that emulsion gel containing cellulose nanofibers has slightly larger droplet diameter, better viscoelasticity and emulsification.
34896953	4	37	contain	has	511:513	arg2	emulsification					576:589	emulsification	576:589	emulsification	576:589	Results showed that emulsion gel containing cellulose nanofibers has slightly larger droplet diameter, better viscoelasticity and emulsification.
34896953	4	37	contain	has	511:513	arg1	nanofibers					500:509	emulsion gel containing cellulose nanofibers	466:509	emulsion gel containing cellulose nanofibers	466:509	Results showed that emulsion gel containing cellulose nanofibers has slightly larger droplet diameter, better viscoelasticity and emulsification.
34896953	4	37	contain	has	511:513	arg2	viscoelasticity					556:570	better viscoelasticity	549:570	better viscoelasticity	549:570	Results showed that emulsion gel containing cellulose nanofibers has slightly larger droplet diameter, better viscoelasticity and emulsification.
34896953	0	38	theme	aerogel	52:58	arg1	fabrication					7:17	Facile fabrication	0:17	Facile fabrication of multifunctional citrus pectin aerogel fortified with cellulose	0:83	Facile fabrication of multifunctional citrus pectin aerogel fortified with cellulose nanofiber as controlled packaging of edible fungi.
34896953	8	39	contain	has	866:868	arg1	aerogel					858:864	aerogel	858:864	aerogel	858:864	Compared with Escherichia coli, aerogel has better resistance to Staphylococcus aureus.
34896953	8	39	contain	has	866:868	arg2	resistance					877:886	resistance	877:886	resistance to Staphylococcus aureus	877:911	Compared with Escherichia coli, aerogel has better resistance to Staphylococcus aureus.
34896953	7	40	theme	Moisture	741:748	arg1	absorption					750:759	Moisture absorption	741:759	Moisture absorption	741:759	Moisture absorption was close to 100% of its own weight, thymol was released slowly.
34896953	7	41	dep	100	774:776	arg1	to					771:772	to	771:772	to	771:772	Moisture absorption was close to 100% of its own weight, thymol was released slowly.
34896953	11	42	theme	fresh-keeping	1169:1181	arg1	period					1183:1188	fresh-keeping period	1169:1188	fresh-keeping period	1169:1188	Hardness, color, total phenol content, cell membrane integrity and total antioxidant capacity of Agaricus bisporus were maintained and fresh-keeping period was extended to 5 days.
34896953	1	43	theme	Citrus	136:141	arg1	agent					216:220	a reinforcing agent	202:220	a reinforcing agent	202:220	Citrus pectin was used as a precursor and cellulose nanofibers as a reinforcing agent, a mixed aerogel with enhanced structural properties was prepared.
34896953	1	43	theme	Citrus	136:141	arg1	precursor					164:172	a precursor	162:172	a precursor	162:172	Citrus pectin was used as a precursor and cellulose nanofibers as a reinforcing agent, a mixed aerogel with enhanced structural properties was prepared.
34896953	1	43	theme	Citrus	136:141	arg1	pectin					143:148	Citrus pectin	136:148	Citrus pectin	136:148	Citrus pectin was used as a precursor and cellulose nanofibers as a reinforcing agent, a mixed aerogel with enhanced structural properties was prepared.
34896953	1	43	theme	Citrus	136:141	arg1	nanofibers					188:197	cellulose nanofibers	178:197	cellulose nanofibers	178:197	Citrus pectin was used as a precursor and cellulose nanofibers as a reinforcing agent, a mixed aerogel with enhanced structural properties was prepared.
34896953	7	44	theme	weight	790:795	arg1	%					777:777	close to 100%	765:777	close to 100% of its own weight	765:795	Moisture absorption was close to 100% of its own weight, thymol was released slowly.
34896953	7	44	theme	weight	790:795	arg1	weight					790:795	its own weight	782:795	its own weight	782:795	Moisture absorption was close to 100% of its own weight, thymol was released slowly.
34896953	6	45	theme	compressive	684:694	arg1	properties					696:705	its tensile and compressive properties	668:705	its tensile and compressive properties	668:705	Additionally, its tensile and compressive properties have been significantly improved.
34896953	2	46	theme	aerogel	327:333	arg1	formation					335:343	aerogel formation	327:343	aerogel formation	327:343	Pickering emulsion was a template for aerogel formation, embedding thymol.
34896953	2	46	theme	aerogel	327:333	arg1	thymol					356:361	embedding thymol	346:361	embedding thymol	346:361	Pickering emulsion was a template for aerogel formation, embedding thymol.
34896953	5	47	contain	has	610:612	arg2	wall					648:651	thinner pore wall	635:651	thinner pore wall	635:651	Composite aerogel has larger pore size and thinner pore wall.
34896953	5	47	contain	has	610:612	arg1	aerogel					602:608	Composite aerogel	592:608	Composite aerogel	592:608	Composite aerogel has larger pore size and thinner pore wall.
34896953	5	47	contain	has	610:612	arg2	size					626:629	larger pore size	614:629	larger pore size	614:629	Composite aerogel has larger pore size and thinner pore wall.
34896953	1	48	with	aerogel	231:237	arg1	properties					264:273	enhanced structural properties	244:273	enhanced structural properties	244:273	Citrus pectin was used as a precursor and cellulose nanofibers as a reinforcing agent, a mixed aerogel with enhanced structural properties was prepared.
34896953	11	49	theme	phenol	1057:1062	arg1	content					1064:1070	total phenol content	1051:1070	total phenol content	1051:1070	Hardness, color, total phenol content, cell membrane integrity and total antioxidant capacity of Agaricus bisporus were maintained and fresh-keeping period was extended to 5 days.
34896953	1	50	theme	mixed	225:229	arg1	aerogel					231:237	a mixed aerogel	223:237	a mixed aerogel with enhanced structural properties	223:273	Citrus pectin was used as a precursor and cellulose nanofibers as a reinforcing agent, a mixed aerogel with enhanced structural properties was prepared.
34896953	4	51	theme	droplet	531:537	arg1	emulsification					576:589	emulsification	576:589	emulsification	576:589	Results showed that emulsion gel containing cellulose nanofibers has slightly larger droplet diameter, better viscoelasticity and emulsification.
34896953	4	51	theme	droplet	531:537	arg1	diameter					539:546	slightly larger droplet diameter	515:546	slightly larger droplet diameter	515:546	Results showed that emulsion gel containing cellulose nanofibers has slightly larger droplet diameter, better viscoelasticity and emulsification.
34896953	4	51	theme	droplet	531:537	arg1	viscoelasticity					556:570	better viscoelasticity	549:570	better viscoelasticity	549:570	Results showed that emulsion gel containing cellulose nanofibers has slightly larger droplet diameter, better viscoelasticity and emulsification.
34896953	6	52	theme	tensile	672:678	arg1	properties					696:705	its tensile and compressive properties	668:705	its tensile and compressive properties	668:705	Additionally, its tensile and compressive properties have been significantly improved.
34896953	7	53	theme	own	786:788	arg1	weight					790:795	its own weight	782:795	its own weight	782:795	Moisture absorption was close to 100% of its own weight, thymol was released slowly.
32716443	6	0	theme	rRNA	1019:1022	arg1	sequencing					1029:1038	16S rRNA gene sequencing	1015:1038	16S rRNA gene sequencing analysis	1015:1047	Moreover, 16S rRNA gene sequencing analysis indicated that SFP treatment could distinctly restore the beneficial composition of gut flora in diabetic rats.
32716443	7	1	dep	absorption	1277:1286	arg1	the					1273:1275	the	1273:1275	the	1273:1275	Furthermore, RT-qPCR analysis revealed that anti-diabetic effects of SFP may be closely related to accelerating the absorption and utilization of blood glucose in the liver and muscle and inhibiting hepatic glucose production.
32716443	2	2	theme	potential	407:415	arg1	effects					431:437	the potential anti-diabetic effects	403:437	the potential anti-diabetic effects	403:437	In the current research, the potential anti-diabetic effects and molecular mechanisms of SFP were investigated by classic biochemical analysis, high-throughput sequencing and molecular biology techniques in type 2 diabetic rats.
32716443	7	3	theme	glucose	1313:1319	arg1	utilization					1292:1302	utilization	1292:1302	utilization	1292:1302	Furthermore, RT-qPCR analysis revealed that anti-diabetic effects of SFP may be closely related to accelerating the absorption and utilization of blood glucose in the liver and muscle and inhibiting hepatic glucose production.
32716443	7	3	theme	glucose	1313:1319	arg1	absorption					1277:1286	absorption	1277:1286	absorption	1277:1286	Furthermore, RT-qPCR analysis revealed that anti-diabetic effects of SFP may be closely related to accelerating the absorption and utilization of blood glucose in the liver and muscle and inhibiting hepatic glucose production.
32716443	2	4	theme	biology	563:569	arg1	techniques					571:580	molecular biology techniques	553:580	molecular biology techniques	553:580	In the current research, the potential anti-diabetic effects and molecular mechanisms of SFP were investigated by classic biochemical analysis, high-throughput sequencing and molecular biology techniques in type 2 diabetic rats.
32716443	0	5	from	effects	13:19	arg1	Sargassum					94:102	Sargassum	94:102	Sargassum	94:102	Antidiabetic effects and underlying mechanisms of anti-digestive dietary polysaccharides from Sargassum fusiforme in rats.
32716443	1	6	theme	anti-digestive	169:182	arg1	ingredient					204:213	an anti-digestive biologically active ingredient	166:213	an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction	166:291	Sargassum fusiforme polysaccharides (SFP), an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction, have been proven to exhibit extremely strong alpha-glucosidase inhibitory activity.
32716443	1	6	theme	anti-digestive	169:182	arg1	polysaccharides					143:157	Sargassum fusiforme polysaccharides	123:157	Sargassum fusiforme polysaccharides (SFP)	123:163	Sargassum fusiforme polysaccharides (SFP), an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction, have been proven to exhibit extremely strong alpha-glucosidase inhibitory activity.
32716443	6	7	theme	flora	1137:1141	arg1	composition					1118:1128	the beneficial composition	1103:1128	the beneficial composition of gut flora in diabetic rats	1103:1158	Moreover, 16S rRNA gene sequencing analysis indicated that SFP treatment could distinctly restore the beneficial composition of gut flora in diabetic rats.
32716443	6	8	theme	beneficial	1107:1116	arg1	composition					1118:1128	the beneficial composition	1103:1128	the beneficial composition of gut flora in diabetic rats	1103:1158	Moreover, 16S rRNA gene sequencing analysis indicated that SFP treatment could distinctly restore the beneficial composition of gut flora in diabetic rats.
32716443	7	9	theme	SFP	1230:1232	arg1	effects					1219:1225	anti-diabetic effects	1205:1225	anti-diabetic effects of SFP	1205:1232	Furthermore, RT-qPCR analysis revealed that anti-diabetic effects of SFP may be closely related to accelerating the absorption and utilization of blood glucose in the liver and muscle and inhibiting hepatic glucose production.
32716443	5	10	theme	staining	883:890	arg1	observation					892:902	H&E staining observation	879:902	H&E staining observation	879:902	H&E staining observation confirmed that SFP intervention could partially repair liver and muscle injuries caused by diabetes.
32716443	3	11	theme	symptoms	640:647	arg1	analysis					611:618	The analysis	607:618	The analysis of typical diabetic symptoms and serum profiles	607:666	The analysis of typical diabetic symptoms and serum profiles showed that oral administration of SFP could mitigate hyperglycemia, hyperinsulinemia, dyslipidemia and oxidative stress in diabetic rats.
32716443	3	12	from	hyperglycemia	722:734	arg1	rats					801:804	diabetic rats	792:804	diabetic rats	792:804	The analysis of typical diabetic symptoms and serum profiles showed that oral administration of SFP could mitigate hyperglycemia, hyperinsulinemia, dyslipidemia and oxidative stress in diabetic rats.
32716443	7	13	from	absorption	1277:1286	arg1	muscle					1338:1343	muscle	1338:1343	muscle	1338:1343	Furthermore, RT-qPCR analysis revealed that anti-diabetic effects of SFP may be closely related to accelerating the absorption and utilization of blood glucose in the liver and muscle and inhibiting hepatic glucose production.
32716443	7	13	from	absorption	1277:1286	arg1	liver					1328:1332	liver	1328:1332	liver	1328:1332	Furthermore, RT-qPCR analysis revealed that anti-diabetic effects of SFP may be closely related to accelerating the absorption and utilization of blood glucose in the liver and muscle and inhibiting hepatic glucose production.
32716443	7	14	theme	hepatic	1360:1366	arg1	production					1376:1385	hepatic glucose production	1360:1385	hepatic glucose production	1360:1385	Furthermore, RT-qPCR analysis revealed that anti-diabetic effects of SFP may be closely related to accelerating the absorption and utilization of blood glucose in the liver and muscle and inhibiting hepatic glucose production.
32716443	3	15	theme	typical	623:629	arg1	symptoms					640:647	typical diabetic symptoms	623:647	typical diabetic symptoms	623:647	The analysis of typical diabetic symptoms and serum profiles showed that oral administration of SFP could mitigate hyperglycemia, hyperinsulinemia, dyslipidemia and oxidative stress in diabetic rats.
32716443	2	16	theme	diabetic	592:599	arg1	rats					601:604	type 2 diabetic rats	585:604	type 2 diabetic rats	585:604	In the current research, the potential anti-diabetic effects and molecular mechanisms of SFP were investigated by classic biochemical analysis, high-throughput sequencing and molecular biology techniques in type 2 diabetic rats.
32716443	2	17	theme	high-throughput	522:536	arg1	sequencing					538:547	high-throughput sequencing	522:547	high-throughput sequencing	522:547	In the current research, the potential anti-diabetic effects and molecular mechanisms of SFP were investigated by classic biochemical analysis, high-throughput sequencing and molecular biology techniques in type 2 diabetic rats.
32716443	7	18	from	utilization	1292:1302	arg1	muscle					1338:1343	muscle	1338:1343	muscle	1338:1343	Furthermore, RT-qPCR analysis revealed that anti-diabetic effects of SFP may be closely related to accelerating the absorption and utilization of blood glucose in the liver and muscle and inhibiting hepatic glucose production.
32716443	7	18	from	utilization	1292:1302	arg1	liver					1328:1332	liver	1328:1332	liver	1328:1332	Furthermore, RT-qPCR analysis revealed that anti-diabetic effects of SFP may be closely related to accelerating the absorption and utilization of blood glucose in the liver and muscle and inhibiting hepatic glucose production.
32716443	3	19	from	stress	782:787	arg1	rats					801:804	diabetic rats	792:804	diabetic rats	792:804	The analysis of typical diabetic symptoms and serum profiles showed that oral administration of SFP could mitigate hyperglycemia, hyperinsulinemia, dyslipidemia and oxidative stress in diabetic rats.
32716443	4	20	theme	skeletal	861:868	arg1	muscles					870:876	skeletal muscles	861:876	skeletal muscles	861:876	SFP also promoted glycogen synthesis in the liver and skeletal muscles.
32716443	1	21	theme	active	197:202	arg1	ingredient					204:213	an anti-digestive biologically active ingredient	166:213	an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction	166:291	Sargassum fusiforme polysaccharides (SFP), an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction, have been proven to exhibit extremely strong alpha-glucosidase inhibitory activity.
32716443	1	21	theme	active	197:202	arg1	polysaccharides					143:157	Sargassum fusiforme polysaccharides	123:157	Sargassum fusiforme polysaccharides (SFP)	123:163	Sargassum fusiforme polysaccharides (SFP), an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction, have been proven to exhibit extremely strong alpha-glucosidase inhibitory activity.
32716443	0	22	from	mechanisms	36:45	arg1	Sargassum					94:102	Sargassum	94:102	Sargassum	94:102	Antidiabetic effects and underlying mechanisms of anti-digestive dietary polysaccharides from Sargassum fusiforme in rats.
32716443	6	23	from	composition	1118:1128	arg1	rats					1155:1158	diabetic rats	1146:1158	diabetic rats	1146:1158	Moreover, 16S rRNA gene sequencing analysis indicated that SFP treatment could distinctly restore the beneficial composition of gut flora in diabetic rats.
32716443	2	24	theme	molecular	443:451	arg1	mechanisms					453:462	molecular mechanisms	443:462	molecular mechanisms of SFP	443:469	In the current research, the potential anti-diabetic effects and molecular mechanisms of SFP were investigated by classic biochemical analysis, high-throughput sequencing and molecular biology techniques in type 2 diabetic rats.
32716443	0	25	theme	Antidiabetic	0:11	arg1	effects					13:19	Antidiabetic effects	0:19	Antidiabetic effects	0:19	Antidiabetic effects and underlying mechanisms of anti-digestive dietary polysaccharides from Sargassum fusiforme in rats.
32716443	7	26	theme	anti-diabetic	1205:1217	arg1	effects					1219:1225	anti-diabetic effects	1205:1225	anti-diabetic effects of SFP	1205:1232	Furthermore, RT-qPCR analysis revealed that anti-diabetic effects of SFP may be closely related to accelerating the absorption and utilization of blood glucose in the liver and muscle and inhibiting hepatic glucose production.
32716443	3	27	theme	serum	653:657	arg1	profiles					659:666	serum profiles	653:666	serum profiles	653:666	The analysis of typical diabetic symptoms and serum profiles showed that oral administration of SFP could mitigate hyperglycemia, hyperinsulinemia, dyslipidemia and oxidative stress in diabetic rats.
32716443	2	28	theme	biochemical	500:510	arg1	analysis					512:519	classic biochemical analysis	492:519	classic biochemical analysis	492:519	In the current research, the potential anti-diabetic effects and molecular mechanisms of SFP were investigated by classic biochemical analysis, high-throughput sequencing and molecular biology techniques in type 2 diabetic rats.
32716443	4	29	theme	glycogen	825:832	arg1	synthesis					834:842	glycogen synthesis	825:842	glycogen synthesis	825:842	SFP also promoted glycogen synthesis in the liver and skeletal muscles.
32716443	3	30	theme	diabetic	631:638	arg1	symptoms					640:647	typical diabetic symptoms	623:647	typical diabetic symptoms	623:647	The analysis of typical diabetic symptoms and serum profiles showed that oral administration of SFP could mitigate hyperglycemia, hyperinsulinemia, dyslipidemia and oxidative stress in diabetic rats.
32716443	8	31	theme	functional	1453:1462	arg1	foods					1464:1468	functional foods	1453:1468	functional foods	1453:1468	In short, this study demonstrated that SFP could be developed as functional foods or pharmaceutical supplements for the prevention or mitigation of diabetes and its complications.
32716443	8	31	theme	functional	1453:1462	arg1	SFP					1427:1429	SFP	1427:1429	SFP	1427:1429	In short, this study demonstrated that SFP could be developed as functional foods or pharmaceutical supplements for the prevention or mitigation of diabetes and its complications.
32716443	2	32	theme	classic	492:498	arg1	analysis					512:519	classic biochemical analysis	492:519	classic biochemical analysis	492:519	In the current research, the potential anti-diabetic effects and molecular mechanisms of SFP were investigated by classic biochemical analysis, high-throughput sequencing and molecular biology techniques in type 2 diabetic rats.
32716443	1	33	theme	strong	332:337	arg1	activity					368:375	extremely strong alpha-glucosidase inhibitory activity	322:375	extremely strong alpha-glucosidase inhibitory activity	322:375	Sargassum fusiforme polysaccharides (SFP), an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction, have been proven to exhibit extremely strong alpha-glucosidase inhibitory activity.
32716443	8	34	dep	prevention	1508:1517	arg1	the					1504:1506	the	1504:1506	the	1504:1506	In short, this study demonstrated that SFP could be developed as functional foods or pharmaceutical supplements for the prevention or mitigation of diabetes and its complications.
32716443	1	35	theme	Sargassum	123:131	arg1	ingredient					204:213	an anti-digestive biologically active ingredient	166:213	an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction	166:291	Sargassum fusiforme polysaccharides (SFP), an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction, have been proven to exhibit extremely strong alpha-glucosidase inhibitory activity.
32716443	1	35	theme	Sargassum	123:131	arg1	SFP					160:162	SFP	160:162	SFP	160:162	Sargassum fusiforme polysaccharides (SFP), an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction, have been proven to exhibit extremely strong alpha-glucosidase inhibitory activity.
32716443	1	35	theme	Sargassum	123:131	arg1	polysaccharides					143:157	Sargassum fusiforme polysaccharides	123:157	Sargassum fusiforme polysaccharides (SFP)	123:163	Sargassum fusiforme polysaccharides (SFP), an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction, have been proven to exhibit extremely strong alpha-glucosidase inhibitory activity.
32716443	1	36	theme	Sargassum	229:237	arg1	fusiforme					239:247	Sargassum fusiforme	229:247	Sargassum fusiforme	229:247	Sargassum fusiforme polysaccharides (SFP), an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction, have been proven to exhibit extremely strong alpha-glucosidase inhibitory activity.
32716443	2	37	theme	molecular	553:561	arg1	techniques					571:580	molecular biology techniques	553:580	molecular biology techniques	553:580	In the current research, the potential anti-diabetic effects and molecular mechanisms of SFP were investigated by classic biochemical analysis, high-throughput sequencing and molecular biology techniques in type 2 diabetic rats.
32716443	1	38	theme	alpha-glucosidase	339:355	arg1	activity					368:375	extremely strong alpha-glucosidase inhibitory activity	322:375	extremely strong alpha-glucosidase inhibitory activity	322:375	Sargassum fusiforme polysaccharides (SFP), an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction, have been proven to exhibit extremely strong alpha-glucosidase inhibitory activity.
32716443	7	39	theme	blood	1307:1311	arg1	glucose					1313:1319	blood glucose	1307:1319	blood glucose	1307:1319	Furthermore, RT-qPCR analysis revealed that anti-diabetic effects of SFP may be closely related to accelerating the absorption and utilization of blood glucose in the liver and muscle and inhibiting hepatic glucose production.
32716443	0	40	from	Sargassum	94:102	arg1	polysaccharides					73:87	anti-digestive dietary polysaccharides	50:87	anti-digestive dietary polysaccharides from Sargassum	50:102	Antidiabetic effects and underlying mechanisms of anti-digestive dietary polysaccharides from Sargassum fusiforme in rats.
32716443	0	40	from	Sargassum	94:102	arg1	effects					13:19	Antidiabetic effects	0:19	Antidiabetic effects	0:19	Antidiabetic effects and underlying mechanisms of anti-digestive dietary polysaccharides from Sargassum fusiforme in rats.
32716443	0	40	from	Sargassum	94:102	arg1	mechanisms					36:45	underlying mechanisms	25:45	underlying mechanisms	25:45	Antidiabetic effects and underlying mechanisms of anti-digestive dietary polysaccharides from Sargassum fusiforme in rats.
32716443	6	41	theme	SFP	1064:1066	arg1	treatment					1068:1076	SFP treatment	1064:1076	SFP treatment	1064:1076	Moreover, 16S rRNA gene sequencing analysis indicated that SFP treatment could distinctly restore the beneficial composition of gut flora in diabetic rats.
32716443	5	42	theme	H&E	879:881	arg1	staining					883:890	H&E staining	879:890	H&E staining observation	879:902	H&E staining observation confirmed that SFP intervention could partially repair liver and muscle injuries caused by diabetes.
32716443	1	43	theme	inhibitory	357:366	arg1	activity					368:375	extremely strong alpha-glucosidase inhibitory activity	322:375	extremely strong alpha-glucosidase inhibitory activity	322:375	Sargassum fusiforme polysaccharides (SFP), an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction, have been proven to exhibit extremely strong alpha-glucosidase inhibitory activity.
32716443	7	44	theme	RT-qPCR	1174:1180	arg1	analysis					1182:1189	RT-qPCR analysis	1174:1189	RT-qPCR analysis	1174:1189	Furthermore, RT-qPCR analysis revealed that anti-diabetic effects of SFP may be closely related to accelerating the absorption and utilization of blood glucose in the liver and muscle and inhibiting hepatic glucose production.
32716443	0	45	theme	underlying	25:34	arg1	mechanisms					36:45	underlying mechanisms	25:45	underlying mechanisms	25:45	Antidiabetic effects and underlying mechanisms of anti-digestive dietary polysaccharides from Sargassum fusiforme in rats.
32716443	2	46	theme	SFP	467:469	arg1	mechanisms					453:462	molecular mechanisms	443:462	molecular mechanisms of SFP	443:469	In the current research, the potential anti-diabetic effects and molecular mechanisms of SFP were investigated by classic biochemical analysis, high-throughput sequencing and molecular biology techniques in type 2 diabetic rats.
32716443	2	46	theme	SFP	467:469	arg1	effects					431:437	the potential anti-diabetic effects	403:437	the potential anti-diabetic effects	403:437	In the current research, the potential anti-diabetic effects and molecular mechanisms of SFP were investigated by classic biochemical analysis, high-throughput sequencing and molecular biology techniques in type 2 diabetic rats.
32716443	0	47	theme	dietary	65:71	arg1	polysaccharides					73:87	anti-digestive dietary polysaccharides	50:87	anti-digestive dietary polysaccharides from Sargassum	50:102	Antidiabetic effects and underlying mechanisms of anti-digestive dietary polysaccharides from Sargassum fusiforme in rats.
32716443	6	48	theme	diabetic	1146:1153	arg1	rats					1155:1158	diabetic rats	1146:1158	diabetic rats	1146:1158	Moreover, 16S rRNA gene sequencing analysis indicated that SFP treatment could distinctly restore the beneficial composition of gut flora in diabetic rats.
32716443	3	49	from	dyslipidemia	755:766	arg1	rats					801:804	diabetic rats	792:804	diabetic rats	792:804	The analysis of typical diabetic symptoms and serum profiles showed that oral administration of SFP could mitigate hyperglycemia, hyperinsulinemia, dyslipidemia and oxidative stress in diabetic rats.
32716443	3	50	theme	profiles	659:666	arg1	analysis					611:618	The analysis	607:618	The analysis of typical diabetic symptoms and serum profiles	607:666	The analysis of typical diabetic symptoms and serum profiles showed that oral administration of SFP could mitigate hyperglycemia, hyperinsulinemia, dyslipidemia and oxidative stress in diabetic rats.
32716443	8	51	theme	pharmaceutical	1473:1486	arg1	supplements					1488:1498	pharmaceutical supplements	1473:1498	pharmaceutical supplements	1473:1498	In short, this study demonstrated that SFP could be developed as functional foods or pharmaceutical supplements for the prevention or mitigation of diabetes and its complications.
32716443	8	51	theme	pharmaceutical	1473:1486	arg1	SFP					1427:1429	SFP	1427:1429	SFP	1427:1429	In short, this study demonstrated that SFP could be developed as functional foods or pharmaceutical supplements for the prevention or mitigation of diabetes and its complications.
32716443	1	52	theme	fusiforme	133:141	arg1	ingredient					204:213	an anti-digestive biologically active ingredient	166:213	an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction	166:291	Sargassum fusiforme polysaccharides (SFP), an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction, have been proven to exhibit extremely strong alpha-glucosidase inhibitory activity.
32716443	1	52	theme	fusiforme	133:141	arg1	SFP					160:162	SFP	160:162	SFP	160:162	Sargassum fusiforme polysaccharides (SFP), an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction, have been proven to exhibit extremely strong alpha-glucosidase inhibitory activity.
32716443	1	52	theme	fusiforme	133:141	arg1	polysaccharides					143:157	Sargassum fusiforme polysaccharides	123:157	Sargassum fusiforme polysaccharides (SFP)	123:163	Sargassum fusiforme polysaccharides (SFP), an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction, have been proven to exhibit extremely strong alpha-glucosidase inhibitory activity.
32716443	0	53	theme	anti-digestive	50:63	arg1	polysaccharides					73:87	anti-digestive dietary polysaccharides	50:87	anti-digestive dietary polysaccharides from Sargassum	50:102	Antidiabetic effects and underlying mechanisms of anti-digestive dietary polysaccharides from Sargassum fusiforme in rats.
32716443	6	54	theme	gene	1024:1027	arg1	sequencing					1029:1038	16S rRNA gene sequencing	1015:1038	16S rRNA gene sequencing analysis	1015:1047	Moreover, 16S rRNA gene sequencing analysis indicated that SFP treatment could distinctly restore the beneficial composition of gut flora in diabetic rats.
32716443	6	55	theme	16S	1015:1017	arg1	sequencing					1029:1038	16S rRNA gene sequencing	1015:1038	16S rRNA gene sequencing analysis	1015:1047	Moreover, 16S rRNA gene sequencing analysis indicated that SFP treatment could distinctly restore the beneficial composition of gut flora in diabetic rats.
32716443	5	56	theme	SFP	919:921	arg1	intervention					923:934	SFP intervention	919:934	SFP intervention	919:934	H&E staining observation confirmed that SFP intervention could partially repair liver and muscle injuries caused by diabetes.
32716443	3	57	from	hyperinsulinemia	737:752	arg1	rats					801:804	diabetic rats	792:804	diabetic rats	792:804	The analysis of typical diabetic symptoms and serum profiles showed that oral administration of SFP could mitigate hyperglycemia, hyperinsulinemia, dyslipidemia and oxidative stress in diabetic rats.
32716443	6	58	theme	sequencing	1029:1038	arg1	analysis					1040:1047	16S rRNA gene sequencing analysis	1015:1047	16S rRNA gene sequencing analysis	1015:1047	Moreover, 16S rRNA gene sequencing analysis indicated that SFP treatment could distinctly restore the beneficial composition of gut flora in diabetic rats.
32716443	4	59	dep	liver	851:855	arg1	the					847:849	the	847:849	the	847:849	SFP also promoted glycogen synthesis in the liver and skeletal muscles.
32716443	3	60	theme	oxidative	772:780	arg1	stress					782:787	oxidative stress	772:787	oxidative stress	772:787	The analysis of typical diabetic symptoms and serum profiles showed that oral administration of SFP could mitigate hyperglycemia, hyperinsulinemia, dyslipidemia and oxidative stress in diabetic rats.
32716443	3	61	theme	SFP	703:705	arg1	administration					685:698	oral administration	680:698	oral administration of SFP	680:705	The analysis of typical diabetic symptoms and serum profiles showed that oral administration of SFP could mitigate hyperglycemia, hyperinsulinemia, dyslipidemia and oxidative stress in diabetic rats.
32716443	3	62	theme	oral	680:683	arg1	administration					685:698	oral administration	680:698	oral administration of SFP	680:705	The analysis of typical diabetic symptoms and serum profiles showed that oral administration of SFP could mitigate hyperglycemia, hyperinsulinemia, dyslipidemia and oxidative stress in diabetic rats.
32716443	2	63	theme	current	385:391	arg1	research					393:400	the current research	381:400	the current research	381:400	In the current research, the potential anti-diabetic effects and molecular mechanisms of SFP were investigated by classic biochemical analysis, high-throughput sequencing and molecular biology techniques in type 2 diabetic rats.
32716443	1	64	theme	ultrasound-assisted	252:270	arg1	extraction					282:291	ultrasound-assisted enzymatic extraction	252:291	ultrasound-assisted enzymatic extraction	252:291	Sargassum fusiforme polysaccharides (SFP), an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction, have been proven to exhibit extremely strong alpha-glucosidase inhibitory activity.
32716443	8	65	theme	diabetes	1536:1543	arg1	mitigation					1522:1531	mitigation	1522:1531	mitigation	1522:1531	In short, this study demonstrated that SFP could be developed as functional foods or pharmaceutical supplements for the prevention or mitigation of diabetes and its complications.
32716443	8	65	theme	diabetes	1536:1543	arg1	prevention					1508:1517	prevention	1508:1517	prevention	1508:1517	In short, this study demonstrated that SFP could be developed as functional foods or pharmaceutical supplements for the prevention or mitigation of diabetes and its complications.
32716443	1	66	theme	enzymatic	272:280	arg1	extraction					282:291	ultrasound-assisted enzymatic extraction	252:291	ultrasound-assisted enzymatic extraction	252:291	Sargassum fusiforme polysaccharides (SFP), an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction, have been proven to exhibit extremely strong alpha-glucosidase inhibitory activity.
32716443	5	67	theme	liver	959:963	arg1	injuries					976:983	liver and muscle injuries	959:983	liver and muscle injuries caused by diabetes	959:1002	H&E staining observation confirmed that SFP intervention could partially repair liver and muscle injuries caused by diabetes.
32716443	8	68	theme	complications	1553:1565	arg1	mitigation					1522:1531	mitigation	1522:1531	mitigation	1522:1531	In short, this study demonstrated that SFP could be developed as functional foods or pharmaceutical supplements for the prevention or mitigation of diabetes and its complications.
32716443	8	68	theme	complications	1553:1565	arg1	prevention					1508:1517	prevention	1508:1517	prevention	1508:1517	In short, this study demonstrated that SFP could be developed as functional foods or pharmaceutical supplements for the prevention or mitigation of diabetes and its complications.
32716443	7	69	theme	glucose	1368:1374	arg1	production					1376:1385	hepatic glucose production	1360:1385	hepatic glucose production	1360:1385	Furthermore, RT-qPCR analysis revealed that anti-diabetic effects of SFP may be closely related to accelerating the absorption and utilization of blood glucose in the liver and muscle and inhibiting hepatic glucose production.
32716443	5	70	theme	muscle	969:974	arg1	injuries					976:983	liver and muscle injuries	959:983	liver and muscle injuries caused by diabetes	959:1002	H&E staining observation confirmed that SFP intervention could partially repair liver and muscle injuries caused by diabetes.
32716443	6	71	theme	gut	1133:1135	arg1	flora					1137:1141	gut flora	1133:1141	gut flora	1133:1141	Moreover, 16S rRNA gene sequencing analysis indicated that SFP treatment could distinctly restore the beneficial composition of gut flora in diabetic rats.
32716443	0	72	theme	polysaccharides	73:87	arg1	effects					13:19	Antidiabetic effects	0:19	Antidiabetic effects	0:19	Antidiabetic effects and underlying mechanisms of anti-digestive dietary polysaccharides from Sargassum fusiforme in rats.
32716443	0	72	theme	polysaccharides	73:87	arg1	mechanisms					36:45	underlying mechanisms	25:45	underlying mechanisms	25:45	Antidiabetic effects and underlying mechanisms of anti-digestive dietary polysaccharides from Sargassum fusiforme in rats.
32716443	3	73	theme	diabetic	792:799	arg1	rats					801:804	diabetic rats	792:804	diabetic rats	792:804	The analysis of typical diabetic symptoms and serum profiles showed that oral administration of SFP could mitigate hyperglycemia, hyperinsulinemia, dyslipidemia and oxidative stress in diabetic rats.
32716443	2	74	theme	anti-diabetic	417:429	arg1	effects					431:437	the potential anti-diabetic effects	403:437	the potential anti-diabetic effects	403:437	In the current research, the potential anti-diabetic effects and molecular mechanisms of SFP were investigated by classic biochemical analysis, high-throughput sequencing and molecular biology techniques in type 2 diabetic rats.
32637953	0	0	theme	Fab-dimerized	69:81	arg1	antibodies					99:108	Fab-dimerized glycan-reactive antibodies	69:108	Fab-dimerized glycan-reactive antibodies	69:108	A glycan cluster on the SARS-CoV-2 spike ectodomain is recognized by Fab-dimerized glycan-reactive antibodies.
32637953	0	1	from	cluster	9:15	arg1	ectodomain					41:50	the SARS-CoV-2 spike ectodomain	20:50	the SARS-CoV-2 spike ectodomain	20:50	A glycan cluster on the SARS-CoV-2 spike ectodomain is recognized by Fab-dimerized glycan-reactive antibodies.
32637953	5	2	theme	S	714:714	arg1	ectodomain					724:733	the S protein ectodomain	710:733	the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12	710:775	A 3.1 Å resolution cryo-EM structure of the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12 revealed a quaternary glycan epitope on the spike S2 domain involving multiple protomers.
32637953	5	3	theme	multiple	847:854	arg1	protomers					856:864	multiple protomers	847:864	multiple protomers	847:864	A 3.1 Å resolution cryo-EM structure of the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12 revealed a quaternary glycan epitope on the spike S2 domain involving multiple protomers.
32637953	5	4	theme	protein	716:722	arg1	ectodomain					724:733	the S protein ectodomain	710:733	the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12	710:775	A 3.1 Å resolution cryo-EM structure of the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12 revealed a quaternary glycan epitope on the spike S2 domain involving multiple protomers.
32637953	7	5	theme	cryo-EM	1075:1081	arg1	structure					1083:1091	SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure	1041:1091	SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure	1041:1091	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	3	6	theme	S	339:339	arg1	protein					341:347	The S protein	335:347	The S protein	335:347	The S protein, like many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin, is glycosylated with both complex and high mannose glycans.
32637953	7	7	theme	SARS-CoV-2	1041:1050	arg1	structure					1083:1091	SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure	1041:1091	SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure	1041:1091	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	7	8	theme	Å	1062:1062	arg1	structure					1083:1091	SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure	1041:1091	SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure	1041:1091	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	2	9	theme	current	264:270	arg1	protein					204:210	The spike (S) protein	190:210	The spike (S) protein that mediates cell entry and membrane fusion	190:255	The spike (S) protein that mediates cell entry and membrane fusion is the current focus of vaccine and therapeutic antibody development efforts.
32637953	2	9	theme	current	264:270	arg1	focus					272:276	the current focus	260:276	the current focus of vaccine and therapeutic antibody development efforts	260:332	The spike (S) protein that mediates cell entry and membrane fusion is the current focus of vaccine and therapeutic antibody development efforts.
32637953	4	10	theme	S	552:552	arg1	protein					554:560	the SARS-CoV-2 S protein	537:560	the SARS-CoV-2 S protein	537:560	Here we demonstrate binding to the SARS-CoV-2 S protein by a category of Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs).
32637953	0	11	theme	glycan-reactive	83:97	arg1	antibodies					99:108	Fab-dimerized glycan-reactive antibodies	69:108	Fab-dimerized glycan-reactive antibodies	69:108	A glycan cluster on the SARS-CoV-2 spike ectodomain is recognized by Fab-dimerized glycan-reactive antibodies.
32637953	7	12	theme	resolution	1064:1073	arg1	structure					1083:1091	SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure	1041:1091	SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure	1041:1091	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	7	13	theme	S2	1112:1113	arg1	epitope					1115:1121	quaternary S2 epitope	1101:1121	quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans	1101:1162	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	4	14	theme	SARS-CoV-2	541:550	arg1	protein					554:560	the SARS-CoV-2 S protein	537:560	the SARS-CoV-2 S protein	537:560	Here we demonstrate binding to the SARS-CoV-2 S protein by a category of Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs).
32637953	5	15	theme	cryo-EM	689:695	arg1	structure					697:705	A 3.1 Å resolution cryo-EM structure	670:705	A 3.1 Å resolution cryo-EM structure of the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12	670:775	A 3.1 Å resolution cryo-EM structure of the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12 revealed a quaternary glycan epitope on the spike S2 domain involving multiple protomers.
32637953	2	16	theme	efforts	326:332	arg1	protein					204:210	The spike (S) protein	190:210	The spike (S) protein that mediates cell entry and membrane fusion	190:255	The spike (S) protein that mediates cell entry and membrane fusion is the current focus of vaccine and therapeutic antibody development efforts.
32637953	2	16	theme	efforts	326:332	arg1	focus					272:276	the current focus	260:276	the current focus of vaccine and therapeutic antibody development efforts	260:332	The spike (S) protein that mediates cell entry and membrane fusion is the current focus of vaccine and therapeutic antibody development efforts.
32637953	5	17	theme	quaternary	788:797	arg1	epitope					806:812	a quaternary glycan epitope	786:812	a quaternary glycan epitope on the spike S2 domain involving multiple protomers	786:864	A 3.1 Å resolution cryo-EM structure of the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12 revealed a quaternary glycan epitope on the spike S2 domain involving multiple protomers.
32637953	5	18	theme	HIV-1	761:765	arg1	2G12					772:775	glycan-dependent HIV-1 bnAb 2G12	744:775	glycan-dependent HIV-1 bnAb 2G12	744:775	A 3.1 Å resolution cryo-EM structure of the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12 revealed a quaternary glycan epitope on the spike S2 domain involving multiple protomers.
32637953	3	19	theme	high	484:487	arg1	glycans					497:503	both complex and high mannose glycans	467:503	glycans	497:503	The S protein, like many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin, is glycosylated with both complex and high mannose glycans.
32637953	5	20	theme	spike	821:825	arg1	domain					830:835	the spike S2 domain	817:835	the spike S2 domain involving multiple protomers	817:864	A 3.1 Å resolution cryo-EM structure of the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12 revealed a quaternary glycan epitope on the spike S2 domain involving multiple protomers.
32637953	3	21	theme	envelope	402:409	arg1	Env					412:414	HIV-1 envelope (Env)	396:415	HIV-1 envelope (Env)	396:415	The S protein, like many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin, is glycosylated with both complex and high mannose glycans.
32637953	0	22	theme	spike	35:39	arg1	ectodomain					41:50	the SARS-CoV-2 spike ectodomain	20:50	the SARS-CoV-2 spike ectodomain	20:50	A glycan cluster on the SARS-CoV-2 spike ectodomain is recognized by Fab-dimerized glycan-reactive antibodies.
32637953	5	23	theme	ectodomain	724:733	arg1	structure					697:705	A 3.1 Å resolution cryo-EM structure	670:705	A 3.1 Å resolution cryo-EM structure of the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12	670:775	A 3.1 Å resolution cryo-EM structure of the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12 revealed a quaternary glycan epitope on the spike S2 domain involving multiple protomers.
32637953	3	24	theme	mannose	489:495	arg1	glycans					497:503	both complex and high mannose glycans	467:503	glycans	497:503	The S protein, like many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin, is glycosylated with both complex and high mannose glycans.
32637953	1	25	theme	global	175:180	arg1	crisis					182:187	a global crisis	173:187	a global crisis	173:187	The COVID-19 pandemic caused by SARS-CoV-2 has escalated into a global crisis.
32637953	0	26	theme	glycan	2:7	arg1	cluster					9:15	A glycan cluster	0:15	A glycan cluster on the SARS-CoV-2 spike ectodomain	0:50	A glycan cluster on the SARS-CoV-2 spike ectodomain is recognized by Fab-dimerized glycan-reactive antibodies.
32637953	3	27	gly	glycosylated	449:460	arg1	protein					341:347	The S protein	335:347	The S protein	335:347	The S protein, like many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin, is glycosylated with both complex and high mannose glycans.
32637953	7	28	theme	SARS-CoV-2	1203:1212	arg1	studies					1224:1230	the SARS-CoV-2 spike.Our studies	1199:1230	the SARS-CoV-2 spike.Our studies	1199:1230	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	4	29	theme	broadly	629:635	arg1	bnAbs					662:666	bnAbs	662:666	bnAbs	662:666	Here we demonstrate binding to the SARS-CoV-2 S protein by a category of Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs).
32637953	4	29	theme	broadly	629:635	arg1	antibodies					650:659	Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies	579:659	Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs)	579:667	Here we demonstrate binding to the SARS-CoV-2 S protein by a category of Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs).
32637953	3	30	theme	other	360:364	arg1	hemagglutinin					431:443	influenza hemagglutinin	421:443	influenza hemagglutinin	421:443	The S protein, like many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin, is glycosylated with both complex and high mannose glycans.
32637953	3	30	theme	other	360:364	arg1	proteins					379:386	many other viral fusion proteins	355:386	many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin	355:443	The S protein, like many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin, is glycosylated with both complex and high mannose glycans.
32637953	3	30	theme	other	360:364	arg1	Env					412:414	HIV-1 envelope (Env)	396:415	HIV-1 envelope (Env)	396:415	The S protein, like many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin, is glycosylated with both complex and high mannose glycans.
32637953	7	31	dep	HIGHLIGHTS	964:973	arg1	cross-react					1024:1034	cross-react	1024:1034	cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709	1024:1301	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	2	32	theme	vaccine	281:287	arg1	protein					204:210	The spike (S) protein	190:210	The spike (S) protein that mediates cell entry and membrane fusion	190:255	The spike (S) protein that mediates cell entry and membrane fusion is the current focus of vaccine and therapeutic antibody development efforts.
32637953	2	32	theme	vaccine	281:287	arg1	focus					272:276	the current focus	260:276	the current focus of vaccine and therapeutic antibody development efforts	260:332	The spike (S) protein that mediates cell entry and membrane fusion is the current focus of vaccine and therapeutic antibody development efforts.
32637953	3	33	theme	HIV-1	396:400	arg1	Env					412:414	HIV-1 envelope (Env)	396:415	HIV-1 envelope (Env)	396:415	The S protein, like many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin, is glycosylated with both complex and high mannose glycans.
32637953	5	34	theme	bnAb	767:770	arg1	2G12					772:775	glycan-dependent HIV-1 bnAb 2G12	744:775	glycan-dependent HIV-1 bnAb 2G12	744:775	A 3.1 Å resolution cryo-EM structure of the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12 revealed a quaternary glycan epitope on the spike S2 domain involving multiple protomers.
32637953	7	35	theme	HIV-1	1012:1016	arg1	bnAbs					1018:1022	Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs	975:1022	Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs	975:1022	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	4	36	theme	Fab-dimerized	579:591	arg1	bnAbs					662:666	bnAbs	662:666	bnAbs	662:666	Here we demonstrate binding to the SARS-CoV-2 S protein by a category of Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs).
32637953	4	36	theme	Fab-dimerized	579:591	arg1	antibodies					650:659	Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies	579:659	Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs)	579:667	Here we demonstrate binding to the SARS-CoV-2 S protein by a category of Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs).
32637953	7	37	theme	HIV-1	1127:1131	arg1	glycans					1156:1162	HIV-1 bnAb 2G12.2G12 targets glycans	1127:1162	HIV-1 bnAb 2G12.2G12 targets glycans	1127:1162	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	6	38	theme	SARS-CoV-2	906:915	arg1	spike					917:921	the SARS-CoV-2 spike	902:921	the SARS-CoV-2 spike that can be targeted for vaccine design	902:961	These data reveal a new epitope on the SARS-CoV-2 spike that can be targeted for vaccine design.
32637953	7	39	theme	Fab-dimerized	975:987	arg1	bnAbs					1018:1022	Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs	975:1022	Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs	975:1022	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	3	40	theme	influenza	421:429	arg1	hemagglutinin					431:443	influenza hemagglutinin	421:443	influenza hemagglutinin	421:443	The S protein, like many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin, is glycosylated with both complex and high mannose glycans.
32637953	0	41	theme	SARS-CoV-2	24:33	arg1	ectodomain					41:50	the SARS-CoV-2 spike ectodomain	20:50	the SARS-CoV-2 spike ectodomain	20:50	A glycan cluster on the SARS-CoV-2 spike ectodomain is recognized by Fab-dimerized glycan-reactive antibodies.
32637953	7	42	theme	2G12.2G12	1138:1146	arg1	glycans					1156:1162	HIV-1 bnAb 2G12.2G12 targets glycans	1127:1162	HIV-1 bnAb 2G12.2G12 targets glycans	1127:1162	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	4	43	theme	HIV-1-induced	615:627	arg1	bnAbs					662:666	bnAbs	662:666	bnAbs	662:666	Here we demonstrate binding to the SARS-CoV-2 S protein by a category of Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs).
32637953	4	43	theme	HIV-1-induced	615:627	arg1	antibodies					650:659	Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies	579:659	Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs)	579:667	Here we demonstrate binding to the SARS-CoV-2 S protein by a category of Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs).
32637953	5	44	theme	glycan	799:804	arg1	epitope					806:812	a quaternary glycan epitope	786:812	a quaternary glycan epitope on the spike S2 domain involving multiple protomers	786:864	A 3.1 Å resolution cryo-EM structure of the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12 revealed a quaternary glycan epitope on the spike S2 domain involving multiple protomers.
32637953	6	45	theme	vaccine	948:954	arg1	design					956:961	vaccine design	948:961	vaccine design	948:961	These data reveal a new epitope on the SARS-CoV-2 spike that can be targeted for vaccine design.
32637953	4	46	theme	glycan-reactive	593:607	arg1	bnAbs					662:666	bnAbs	662:666	bnAbs	662:666	Here we demonstrate binding to the SARS-CoV-2 S protein by a category of Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs).
32637953	4	46	theme	glycan-reactive	593:607	arg1	antibodies					650:659	Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies	579:659	Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs)	579:667	Here we demonstrate binding to the SARS-CoV-2 S protein by a category of Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs).
32637953	3	47	theme	viral	366:370	arg1	hemagglutinin					431:443	influenza hemagglutinin	421:443	influenza hemagglutinin	421:443	The S protein, like many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin, is glycosylated with both complex and high mannose glycans.
32637953	3	47	theme	viral	366:370	arg1	proteins					379:386	many other viral fusion proteins	355:386	many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin	355:443	The S protein, like many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin, is glycosylated with both complex and high mannose glycans.
32637953	3	47	theme	viral	366:370	arg1	Env					412:414	HIV-1 envelope (Env)	396:415	HIV-1 envelope (Env)	396:415	The S protein, like many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin, is glycosylated with both complex and high mannose glycans.
32637953	5	48	theme	Å	676:676	arg1	structure					697:705	A 3.1 Å resolution cryo-EM structure	670:705	A 3.1 Å resolution cryo-EM structure of the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12	670:775	A 3.1 Å resolution cryo-EM structure of the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12 revealed a quaternary glycan epitope on the spike S2 domain involving multiple protomers.
32637953	5	49	theme	glycan-dependent	744:759	arg1	2G12					772:775	glycan-dependent HIV-1 bnAb 2G12	744:775	glycan-dependent HIV-1 bnAb 2G12	744:775	A 3.1 Å resolution cryo-EM structure of the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12 revealed a quaternary glycan epitope on the spike S2 domain involving multiple protomers.
32637953	5	50	from	epitope	806:812	arg1	domain					830:835	the spike S2 domain	817:835	the spike S2 domain involving multiple protomers	817:864	A 3.1 Å resolution cryo-EM structure of the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12 revealed a quaternary glycan epitope on the spike S2 domain involving multiple protomers.
32637953	1	51	theme	COVID-19	115:122	arg1	pandemic					124:131	The COVID-19 pandemic	111:131	The COVID-19 pandemic caused by SARS-CoV-2	111:152	The COVID-19 pandemic caused by SARS-CoV-2 has escalated into a global crisis.
32637953	3	52	theme	fusion	372:377	arg1	hemagglutinin					431:443	influenza hemagglutinin	421:443	influenza hemagglutinin	421:443	The S protein, like many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin, is glycosylated with both complex and high mannose glycans.
32637953	3	52	theme	fusion	372:377	arg1	proteins					379:386	many other viral fusion proteins	355:386	many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin	355:443	The S protein, like many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin, is glycosylated with both complex and high mannose glycans.
32637953	3	52	theme	fusion	372:377	arg1	Env					412:414	HIV-1 envelope (Env)	396:415	HIV-1 envelope (Env)	396:415	The S protein, like many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin, is glycosylated with both complex and high mannose glycans.
32637953	6	53	theme	new	887:889	arg1	epitope					891:897	a new epitope	885:897	a new epitope on the SARS-CoV-2 spike that can be targeted for vaccine design	885:961	These data reveal a new epitope on the SARS-CoV-2 spike that can be targeted for vaccine design.
32637953	7	54	theme	FDG	1261:1263	arg1	antibodies					1265:1274	FDG antibodies	1261:1274	FDG antibodies centered around glycan 709	1261:1301	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	7	55	theme	spike.Our	1214:1222	arg1	studies					1224:1230	the SARS-CoV-2 spike.Our studies	1199:1230	the SARS-CoV-2 spike.Our studies	1199:1230	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	2	56	theme	development	314:324	arg1	efforts					326:332	therapeutic antibody development efforts	293:332	therapeutic antibody development efforts	293:332	The spike (S) protein that mediates cell entry and membrane fusion is the current focus of vaccine and therapeutic antibody development efforts.
32637953	7	57	theme	glycan	1292:1297	arg1	709					1299:1301	glycan 709	1292:1301	glycan 709	1292:1301	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	7	58	theme	quaternary	1101:1110	arg1	epitope					1115:1121	quaternary S2 epitope	1101:1121	quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans	1101:1162	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	2	59	theme	membrane	241:248	arg1	fusion					250:255	membrane fusion	241:255	membrane fusion	241:255	The spike (S) protein that mediates cell entry and membrane fusion is the current focus of vaccine and therapeutic antibody development efforts.
32637953	4	60	theme	neutralizing	637:648	arg1	bnAbs					662:666	bnAbs	662:666	bnAbs	662:666	Here we demonstrate binding to the SARS-CoV-2 S protein by a category of Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs).
32637953	4	60	theme	neutralizing	637:648	arg1	antibodies					650:659	Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies	579:659	Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs)	579:667	Here we demonstrate binding to the SARS-CoV-2 S protein by a category of Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs).
32637953	2	61	theme	antibody	305:312	arg1	efforts					326:332	therapeutic antibody development efforts	293:332	therapeutic antibody development efforts	293:332	The spike (S) protein that mediates cell entry and membrane fusion is the current focus of vaccine and therapeutic antibody development efforts.
32637953	5	62	theme	S2	827:828	arg1	domain					830:835	the spike S2 domain	817:835	the spike S2 domain involving multiple protomers	817:864	A 3.1 Å resolution cryo-EM structure of the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12 revealed a quaternary glycan epitope on the spike S2 domain involving multiple protomers.
32637953	2	63	dep	spike	194:198	arg1	S					201:201	S	201:201	S	201:201	The spike (S) protein that mediates cell entry and membrane fusion is the current focus of vaccine and therapeutic antibody development efforts.
32637953	6	64	from	epitope	891:897	arg1	spike					917:921	the SARS-CoV-2 spike	902:921	the SARS-CoV-2 spike that can be targeted for vaccine design	902:961	These data reveal a new epitope on the SARS-CoV-2 spike that can be targeted for vaccine design.
32637953	2	65	theme	spike	194:198	arg1	protein					204:210	The spike (S) protein	190:210	The spike (S) protein that mediates cell entry and membrane fusion	190:255	The spike (S) protein that mediates cell entry and membrane fusion is the current focus of vaccine and therapeutic antibody development efforts.
32637953	2	65	theme	spike	194:198	arg1	focus					272:276	the current focus	260:276	the current focus of vaccine and therapeutic antibody development efforts	260:332	The spike (S) protein that mediates cell entry and membrane fusion is the current focus of vaccine and therapeutic antibody development efforts.
32637953	7	66	theme	bnAb	1133:1136	arg1	glycans					1156:1162	HIV-1 bnAb 2G12.2G12 targets glycans	1127:1162	HIV-1 bnAb 2G12.2G12 targets glycans	1127:1162	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	2	67	theme	therapeutic	293:303	arg1	efforts					326:332	therapeutic antibody development efforts	293:332	therapeutic antibody development efforts	293:332	The spike (S) protein that mediates cell entry and membrane fusion is the current focus of vaccine and therapeutic antibody development efforts.
32637953	7	68	dep	Fab-dimerized	975:987	arg1	glycan-reactive					990:1004	glycan-reactive	990:1004	glycan-reactive	990:1004	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	7	69	theme	common	1242:1247	arg1	epitope					1249:1255	a common epitope	1240:1255	a common epitope for FDG antibodies centered around glycan 709	1240:1301	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	4	70	theme	antibodies	650:659	arg1	category					567:574	a category	565:574	a category of Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs)	565:667	Here we demonstrate binding to the SARS-CoV-2 S protein by a category of Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs).
32637953	4	71	theme	FDG	610:612	arg1	bnAbs					662:666	bnAbs	662:666	bnAbs	662:666	Here we demonstrate binding to the SARS-CoV-2 S protein by a category of Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs).
32637953	4	71	theme	FDG	610:612	arg1	antibodies					650:659	Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies	579:659	Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs)	579:667	Here we demonstrate binding to the SARS-CoV-2 S protein by a category of Fab-dimerized glycan-reactive (FDG) HIV-1-induced broadly neutralizing antibodies (bnAbs).
32637953	7	72	dep	glycan-reactive	990:1004	arg1	FDG					1007:1009	FDG	1007:1009	FDG	1007:1009	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	7	73	theme	targets	1148:1154	arg1	glycans					1156:1162	HIV-1 bnAb 2G12.2G12 targets glycans	1127:1162	HIV-1 bnAb 2G12.2G12 targets glycans	1127:1162	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	2	74	theme	cell	226:229	arg1	entry					231:235	cell entry	226:235	cell entry	226:235	The spike (S) protein that mediates cell entry and membrane fusion is the current focus of vaccine and therapeutic antibody development efforts.
32637953	3	75	theme	many	355:358	arg1	hemagglutinin					431:443	influenza hemagglutinin	421:443	influenza hemagglutinin	421:443	The S protein, like many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin, is glycosylated with both complex and high mannose glycans.
32637953	3	75	theme	many	355:358	arg1	proteins					379:386	many other viral fusion proteins	355:386	many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin	355:443	The S protein, like many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin, is glycosylated with both complex and high mannose glycans.
32637953	3	75	theme	many	355:358	arg1	Env					412:414	HIV-1 envelope (Env)	396:415	HIV-1 envelope (Env)	396:415	The S protein, like many other viral fusion proteins such as HIV-1 envelope (Env) and influenza hemagglutinin, is glycosylated with both complex and high mannose glycans.
32637953	7	76	dep	reveals	1093:1099	arg1	in					1196:1197	in	1196:1197	in	1196:1197	HIGHLIGHTS Fab-dimerized, glycan-reactive (FDG) HIV-1 bnAbs cross-react with SARS-CoV-2 spike.3.1 Å resolution cryo-EM structure reveals quaternary S2 epitope for HIV-1 bnAb 2G12.2G12 targets glycans, at positions 709, 717 and 801, in the SARS-CoV-2 spike.Our studies suggest a common epitope for FDG antibodies centered around glycan 709.
32637953	5	77	theme	resolution	678:687	arg1	structure					697:705	A 3.1 Å resolution cryo-EM structure	670:705	A 3.1 Å resolution cryo-EM structure of the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12	670:775	A 3.1 Å resolution cryo-EM structure of the S protein ectodomain bound to glycan-dependent HIV-1 bnAb 2G12 revealed a quaternary glycan epitope on the spike S2 domain involving multiple protomers.
34198096	9	0	attach	presented	1709:1717	arg1	d					1880:1880	d 35	1880:1883	d 35 (P = 0.003)	1880:1895	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	9	0	attach	presented	1709:1717	arg1	P = 0.003					1886:1894	P = 0.003	1886:1894	P = 0.003	1886:1894	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	9	0	attach	presented	1709:1717	arg1	acid					1910:1913	succinic acid	1901:1913	succinic acid at d14 (P = 0.041)	1901:1932	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	9	0	attach	presented	1709:1717	arg2	birds					1693:1697	birds	1693:1697	birds fed wheat	1693:1707	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	6	1	theme	ileal	1223:1227	arg1	P = 0.043					1252:1260	P = 0.043	1252:1260	P = 0.043	1252:1260	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	6	1	theme	ileal	1223:1227	arg1	content					1243:1249	lower ileal Lactobacillus content	1217:1249	lower ileal Lactobacillus content (P = 0.043)	1217:1261	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	9	2	dep	acids	1790:1794	arg1	P = 0.007					1833:1841	P = 0.007	1833:1841	P = 0.007	1833:1841	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	9	2	dep	acids	1790:1794	arg1	P = 0.001					1797:1805	P = 0.001	1797:1805	P = 0.001	1797:1805	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	9	2	dep	acids	1790:1794	arg1	<					1810:1810	P < 0.001, P = 0.003 and P = 0.007	1808:1841	<	1810:1810	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	3	3	theme	chain	646:650	arg1	SCFA					664:667	SCFA	664:667	SCFA	664:667	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
34198096	3	3	theme	chain	646:650	arg1	acid					658:661	caecal short chain fatty acid	633:661	caecal short chain fatty acid (SCFA) concentration	633:682	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
34198096	6	4	theme	low	1055:1057	arg1	sNSP					1059:1062	low sNSP	1055:1062	low sNSP	1055:1062	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	1	5	theme	gastrointestinal	265:280	arg1	tract					282:286	the gastrointestinal tract	261:286	the gastrointestinal tract environment	261:298	The aim of this study was to evaluate the importance of considering dietary soluble non-starch polysaccharides (sNSP) when formulating broiler diets, due to their impact on the gastrointestinal tract environment.
34198096	11	6	theme	direct	2260:2265	arg1	impact					2267:2272	a direct impact	2258:2272	a direct impact	2258:2272	These results illustrate that dietary sNSP concentration, as well as its composition, have a direct impact on gastrointestinal viscosity and pH, and fuel beneficial microbial species, resulting in production of SCFA.
34198096	3	7	theme	caecal	633:638	arg1	SCFA					664:667	SCFA	664:667	SCFA	664:667	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
34198096	3	7	theme	caecal	633:638	arg1	acid					658:661	caecal short chain fatty acid	633:661	caecal short chain fatty acid (SCFA) concentration	633:682	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
34198096	9	8	theme	succinic	1781:1788	arg1	acids					1790:1794	acetic, butyric, lactic, and succinic acids	1752:1794	acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively)	1752:1856	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	8	9	theme	high	1561:1564	arg1	level					1571:1575	high sNSP level	1561:1575	high sNSP level	1561:1575	At d 35, total caecal SCFA concentration was greater in birds fed the wheat-based diet with high sNSP level compared to those fed the corn-based diet with high or low sNSP level (P = 0.028).
34198096	2	10	theme	factorial	519:527	arg1	arrangement					529:539	a 2 × 3 factorial arrangement	511:539	a 2 × 3 factorial arrangement of treatments	511:553	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium or low sNSP content, resulting in 6 dietary treatments in a 2 × 3 factorial arrangement of treatments.
34198096	3	11	theme	acid	658:661	arg1	concentration					670:682	caecal short chain fatty acid (SCFA) concentration	633:682	caecal short chain fatty acid (SCFA) concentration	633:682	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
34198096	6	12	theme	propionic	1107:1115	arg1	P = 0.011					1187:1195	P = 0.011	1187:1195	P = 0.011	1187:1195	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	6	12	theme	propionic	1107:1115	arg1	P = 0.007					1173:1181	P = 0.007	1173:1181	P = 0.007	1173:1181	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	6	12	theme	propionic	1107:1115	arg1	concentrations					1146:1159	propionic, isobutyric and valeric acid concentrations	1107:1159	propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively)	1107:1210	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	7	13	dep	SCFA	1333:1336	arg1	concentrations					1378:1391	concentrations	1378:1391	concentrations	1378:1391	This effect was also seen for total SCFA (P = 0.017) and acetic acid (P = 0.005) concentrations in the caeca at d 14, but only in birds fed wheat-, not corn-, based diets.
34198096	7	14	theme	acetic	1354:1359	arg1	P = 0.005					1367:1375	P = 0.005	1367:1375	P = 0.005	1367:1375	This effect was also seen for total SCFA (P = 0.017) and acetic acid (P = 0.005) concentrations in the caeca at d 14, but only in birds fed wheat-, not corn-, based diets.
34198096	7	14	theme	acetic	1354:1359	arg1	acid					1361:1364	acetic acid	1354:1364	acetic acid (P = 0.005)	1354:1376	This effect was also seen for total SCFA (P = 0.017) and acetic acid (P = 0.005) concentrations in the caeca at d 14, but only in birds fed wheat-, not corn-, based diets.
34198096	4	15	theme	high	791:794	arg1	sNSP					796:799	high sNSP	791:799	high sNSP	791:799	Ileal viscosity at d 35 was greater in birds fed high sNSP compared to low sNSP (P = 0.022).
34198096	2	16	dep	broilers	310:317	arg1	birds					332:336	n = 480, 80 birds	320:336	n = 480, 80 birds per treatment	320:350	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium or low sNSP content, resulting in 6 dietary treatments in a 2 × 3 factorial arrangement of treatments.
34198096	9	17	theme	butyric	1760:1766	arg1	acids					1790:1794	acetic, butyric, lactic, and succinic acids	1752:1794	acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively)	1752:1856	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	3	18	theme	microbiota	706:715	arg1	profile					717:723	ileal and caecal microbiota profile	689:723	ileal and caecal microbiota profile	689:723	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
34198096	8	19	theme	corn-based	1603:1612	arg1	diet					1614:1617	the corn-based diet	1599:1617	the corn-based diet	1599:1617	At d 35, total caecal SCFA concentration was greater in birds fed the wheat-based diet with high sNSP level compared to those fed the corn-based diet with high or low sNSP level (P = 0.028).
34198096	5	20	theme	ileal	906:910	arg1	viscosity					912:920	higher ileal viscosity	899:920	higher ileal viscosity (P < 0.001)	899:932	At both d 14 and d 35, birds fed the wheat-based diet presented higher ileal viscosity (P < 0.001) and lower ileal pH (P = 0.027 and P < 0.001, respectively) compared to those fed the corn-based diet.
34198096	5	20	theme	ileal	906:910	arg1	<					925:925	P < 0.001	923:931	P < 0.001	923:931	At both d 14 and d 35, birds fed the wheat-based diet presented higher ileal viscosity (P < 0.001) and lower ileal pH (P = 0.027 and P < 0.001, respectively) compared to those fed the corn-based diet.
34198096	10	21	dep	diets	2109:2113	arg1	diets					2109:2113	wheat-based diets	2097:2113	wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively)	2097:2164	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	10	21	dep	diets	2109:2113	arg1	P					2141:2141	P	2141:2141	P < 0.001, respectively	2141:2163	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	10	21	dep	diets	2109:2113	arg1	P = 0.019					2127:2135	P = 0.019	2127:2135	P = 0.019	2127:2135	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	10	21	dep	diets	2109:2113	arg1	P = 0.043					2116:2124	P = 0.043	2116:2124	P = 0.043	2116:2124	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	4	22	theme	low	813:815	arg1	P = 0.022					823:831	P = 0.022	823:831	P = 0.022	823:831	Ileal viscosity at d 35 was greater in birds fed high sNSP compared to low sNSP (P = 0.022).
34198096	4	22	theme	low	813:815	arg1	sNSP					817:820	low sNSP	813:820	low sNSP (P = 0.022)	813:832	Ileal viscosity at d 35 was greater in birds fed high sNSP compared to low sNSP (P = 0.022).
34198096	1	23	theme	dietary	156:162	arg1	sNSP					200:203	sNSP	200:203	sNSP	200:203	The aim of this study was to evaluate the importance of considering dietary soluble non-starch polysaccharides (sNSP) when formulating broiler diets, due to their impact on the gastrointestinal tract environment.
34198096	1	23	theme	dietary	156:162	arg1	polysaccharides					183:197	dietary soluble non-starch polysaccharides	156:197	dietary soluble non-starch polysaccharides (sNSP)	156:204	The aim of this study was to evaluate the importance of considering dietary soluble non-starch polysaccharides (sNSP) when formulating broiler diets, due to their impact on the gastrointestinal tract environment.
34198096	7	24	theme	total	1327:1331	arg1	P = 0.017					1339:1347	P = 0.017	1339:1347	P = 0.017	1339:1347	This effect was also seen for total SCFA (P = 0.017) and acetic acid (P = 0.005) concentrations in the caeca at d 14, but only in birds fed wheat-, not corn-, based diets.
34198096	7	24	theme	total	1327:1331	arg1	SCFA					1333:1336	total SCFA	1327:1336	total SCFA (P = 0.017)	1327:1348	This effect was also seen for total SCFA (P = 0.017) and acetic acid (P = 0.005) concentrations in the caeca at d 14, but only in birds fed wheat-, not corn-, based diets.
34198096	6	25	theme	caecal	1081:1086	arg1	P = 0.010					1092:1100	P = 0.010	1092:1100	P = 0.010	1092:1100	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	6	25	theme	caecal	1081:1086	arg1	pH					1088:1089	higher caecal pH	1074:1089	higher caecal pH (P = 0.010)	1074:1101	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	10	26	from	concentrations	1983:1996	arg1	d					2044:2044	d 35	2044:2047	d 35	2044:2047	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	10	26	from	concentrations	1983:1996	arg1	d14					2017:2019	d14	2017:2019	d14	2017:2019	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	8	27	theme	total	1478:1482	arg1	concentration					1496:1508	total caecal SCFA concentration	1478:1508	total caecal SCFA concentration	1478:1508	At d 35, total caecal SCFA concentration was greater in birds fed the wheat-based diet with high sNSP level compared to those fed the corn-based diet with high or low sNSP level (P = 0.028).
34198096	1	28	theme	non-starch	172:181	arg1	sNSP					200:203	sNSP	200:203	sNSP	200:203	The aim of this study was to evaluate the importance of considering dietary soluble non-starch polysaccharides (sNSP) when formulating broiler diets, due to their impact on the gastrointestinal tract environment.
34198096	1	28	theme	non-starch	172:181	arg1	polysaccharides					183:197	dietary soluble non-starch polysaccharides	156:197	dietary soluble non-starch polysaccharides (sNSP)	156:204	The aim of this study was to evaluate the importance of considering dietary soluble non-starch polysaccharides (sNSP) when formulating broiler diets, due to their impact on the gastrointestinal tract environment.
34198096	10	29	theme	concentrations	1983:1996	arg1	populations					1951:1961	caecal populations	1944:1961	caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35	1944:2047	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	10	30	theme	acid	2036:2039	arg1	Ruminococcus					1966:1977	Ruminococcus	1966:1977	Ruminococcus	1966:1977	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	10	30	theme	acid	2036:2039	arg1	concentrations					1983:1996	concentrations	1983:1996	concentrations	1983:1996	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	7	31	dep	wheat-	1437:1442	arg1	based					1456:1460	based	1456:1460	based	1456:1460	This effect was also seen for total SCFA (P = 0.017) and acetic acid (P = 0.005) concentrations in the caeca at d 14, but only in birds fed wheat-, not corn-, based diets.
34198096	7	31	dep	wheat-	1437:1442	arg1	corn-					1449:1453	corn-	1449:1453	corn-	1449:1453	This effect was also seen for total SCFA (P = 0.017) and acetic acid (P = 0.005) concentrations in the caeca at d 14, but only in birds fed wheat-, not corn-, based diets.
34198096	8	32	theme	sNSP	1636:1639	arg1	level					1641:1645	high or low sNSP level	1624:1645	high or low sNSP level (P = 0.028)	1624:1657	At d 35, total caecal SCFA concentration was greater in birds fed the wheat-based diet with high sNSP level compared to those fed the corn-based diet with high or low sNSP level (P = 0.028).
34198096	8	32	theme	sNSP	1636:1639	arg1	P = 0.028					1648:1656	P = 0.028	1648:1656	P = 0.028	1648:1656	At d 35, total caecal SCFA concentration was greater in birds fed the wheat-based diet with high sNSP level compared to those fed the corn-based diet with high or low sNSP level (P = 0.028).
34198096	8	33	theme	SCFA	1491:1494	arg1	concentration					1496:1508	total caecal SCFA concentration	1478:1508	total caecal SCFA concentration	1478:1508	At d 35, total caecal SCFA concentration was greater in birds fed the wheat-based diet with high sNSP level compared to those fed the corn-based diet with high or low sNSP level (P = 0.028).
34198096	10	34	theme	Ruminococcus	1966:1977	arg1	populations					1951:1961	caecal populations	1944:1961	caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35	1944:2047	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	10	35	from	d14	2017:2019	arg1	acid					2009:2012	valeric acid	2001:2012	valeric acid at d14	2001:2019	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	10	35	from	d14	2017:2019	arg1	Ruminococcus					1966:1977	Ruminococcus	1966:1977	Ruminococcus	1966:1977	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	10	35	from	d14	2017:2019	arg1	acid					2036:2039	isobutyric acid	2025:2039	isobutyric acid at d 35	2025:2047	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	10	35	from	d14	2017:2019	arg1	concentrations					1983:1996	concentrations	1983:1996	concentrations	1983:1996	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	2	36	theme	corn-soybean	379:390	arg1	diets					403:407	either wheat- or corn-soybean meal-based diets	362:407	either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium or low sNSP content, resulting in 6 dietary treatments in a 2 × 3 factorial arrangement of treatments	362:553	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium or low sNSP content, resulting in 6 dietary treatments in a 2 × 3 factorial arrangement of treatments.
34198096	5	37	theme	corn-based	1019:1028	arg1	diet					1030:1033	the corn-based diet	1015:1033	the corn-based diet	1015:1033	At both d 14 and d 35, birds fed the wheat-based diet presented higher ileal viscosity (P < 0.001) and lower ileal pH (P = 0.027 and P < 0.001, respectively) compared to those fed the corn-based diet.
34198096	5	38	theme	lower	938:942	arg1	pH					950:951	lower ileal pH	938:951	lower ileal pH (P = 0.027 and P < 0.001, respectively)	938:991	At both d 14 and d 35, birds fed the wheat-based diet presented higher ileal viscosity (P < 0.001) and lower ileal pH (P = 0.027 and P < 0.001, respectively) compared to those fed the corn-based diet.
34198096	6	39	dep	concentrations	1146:1159	arg1	P = 0.011					1187:1195	P = 0.011	1187:1195	P = 0.011	1187:1195	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	6	39	dep	concentrations	1146:1159	arg1	P = 0.007					1173:1181	P = 0.007	1173:1181	P = 0.007	1173:1181	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	6	39	dep	concentrations	1146:1159	arg1	concentrations					1146:1159	propionic, isobutyric and valeric acid concentrations	1107:1159	propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively)	1107:1210	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	2	40	theme	high	440:443	arg1	content					465:471	either a high, medium or low sNSP content	431:471	content	465:471	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium or low sNSP content, resulting in 6 dietary treatments in a 2 × 3 factorial arrangement of treatments.
34198096	9	41	theme	lactic	1769:1774	arg1	acids					1790:1794	acetic, butyric, lactic, and succinic acids	1752:1794	acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively)	1752:1856	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	2	42	theme	wheat-	369:374	arg1	diets					403:407	either wheat- or corn-soybean meal-based diets	362:407	either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium or low sNSP content, resulting in 6 dietary treatments in a 2 × 3 factorial arrangement of treatments	362:553	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium or low sNSP content, resulting in 6 dietary treatments in a 2 × 3 factorial arrangement of treatments.
34198096	0	43	theme	Soluble	0:6	arg1	polysaccharide					19:32	Soluble non-starch polysaccharide	0:32	Soluble non-starch polysaccharide	0:32	Soluble non-starch polysaccharide modulates broiler gastrointestinal tract environment.
34198096	1	44	theme	tract	282:286	arg1	environment					288:298	the gastrointestinal tract environment	261:298	the gastrointestinal tract environment	261:298	The aim of this study was to evaluate the importance of considering dietary soluble non-starch polysaccharides (sNSP) when formulating broiler diets, due to their impact on the gastrointestinal tract environment.
34198096	1	45	theme	study	104:108	arg1	aim					92:94	The aim	88:94	The aim of this study	88:108	The aim of this study was to evaluate the importance of considering dietary soluble non-starch polysaccharides (sNSP) when formulating broiler diets, due to their impact on the gastrointestinal tract environment.
34198096	10	46	theme	valeric	2001:2007	arg1	acid					2009:2012	valeric acid	2001:2012	valeric acid at d14	2001:2019	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	11	47	theme	SCFA	2378:2381	arg1	production					2364:2373	production	2364:2373	production of SCFA	2364:2381	These results illustrate that dietary sNSP concentration, as well as its composition, have a direct impact on gastrointestinal viscosity and pH, and fuel beneficial microbial species, resulting in production of SCFA.
34198096	5	48	attach	presented	889:897	arg1	d					843:843	d 14	843:846	d 14	843:846	At both d 14 and d 35, birds fed the wheat-based diet presented higher ileal viscosity (P < 0.001) and lower ileal pH (P = 0.027 and P < 0.001, respectively) compared to those fed the corn-based diet.
34198096	5	48	attach	presented	889:897	arg1	d					852:852	d 35	852:855	d 35	852:855	At both d 14 and d 35, birds fed the wheat-based diet presented higher ileal viscosity (P < 0.001) and lower ileal pH (P = 0.027 and P < 0.001, respectively) compared to those fed the corn-based diet.
34198096	5	48	attach	presented	889:897	arg2	birds					858:862	birds	858:862	birds fed the wheat-based diet	858:887	At both d 14 and d 35, birds fed the wheat-based diet presented higher ileal viscosity (P < 0.001) and lower ileal pH (P = 0.027 and P < 0.001, respectively) compared to those fed the corn-based diet.
34198096	2	49	theme	low	456:458	arg1	content					465:471	either a high, medium or low sNSP content	431:471	content	465:471	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium or low sNSP content, resulting in 6 dietary treatments in a 2 × 3 factorial arrangement of treatments.
34198096	3	50	theme	gizzard	590:596	arg1	pH					580:581	pH	580:581	pH of the gizzard	580:596	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
34198096	3	50	theme	gizzard	590:596	arg1	profile					717:723	ileal and caecal microbiota profile	689:723	ileal and caecal microbiota profile	689:723	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
34198096	3	50	theme	gizzard	590:596	arg1	concentration					670:682	caecal short chain fatty acid (SCFA) concentration	633:682	caecal short chain fatty acid (SCFA) concentration	633:682	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
34198096	3	50	theme	gizzard	590:596	arg1	viscosity					622:630	ileum viscosity	616:630	ileum viscosity	616:630	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
34198096	3	50	theme	gizzard	590:596	arg1	ileum					599:603	ileum	599:603	ileum	599:603	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
34198096	3	50	theme	gizzard	590:596	arg1	caeca					609:613	caeca	609:613	caeca	609:613	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
34198096	8	51	with	diet	1551:1554	arg1	level					1571:1575	high sNSP level	1561:1575	high sNSP level	1561:1575	At d 35, total caecal SCFA concentration was greater in birds fed the wheat-based diet with high sNSP level compared to those fed the corn-based diet with high or low sNSP level (P = 0.028).
34198096	0	52	theme	broiler	44:50	arg1	environment					75:85	broiler gastrointestinal tract environment	44:85	broiler gastrointestinal tract environment	44:85	Soluble non-starch polysaccharide modulates broiler gastrointestinal tract environment.
34198096	10	53	theme	isobutyric	2025:2034	arg1	acid					2036:2039	isobutyric acid	2025:2039	isobutyric acid at d 35	2025:2047	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	2	54	theme	medium	446:451	arg1	content					465:471	either a high, medium or low sNSP content	431:471	content	465:471	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium or low sNSP content, resulting in 6 dietary treatments in a 2 × 3 factorial arrangement of treatments.
34198096	11	55	theme	sNSP	2205:2208	arg1	concentration					2210:2222	dietary sNSP concentration	2197:2222	dietary sNSP concentration	2197:2222	These results illustrate that dietary sNSP concentration, as well as its composition, have a direct impact on gastrointestinal viscosity and pH, and fuel beneficial microbial species, resulting in production of SCFA.
34198096	4	56	theme	Ileal	742:746	arg1	viscosity					748:756	Ileal viscosity	742:756	Ileal viscosity at d 35	742:764	Ileal viscosity at d 35 was greater in birds fed high sNSP compared to low sNSP (P = 0.022).
34198096	9	57	theme	succinic	1901:1908	arg1	acid					1910:1913	succinic acid	1901:1913	succinic acid at d14 (P = 0.041)	1901:1932	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	0	58	theme	tract	69:73	arg1	environment					75:85	broiler gastrointestinal tract environment	44:85	broiler gastrointestinal tract environment	44:85	Soluble non-starch polysaccharide modulates broiler gastrointestinal tract environment.
34198096	9	59	theme	greater	1719:1725	arg1	concentrations					1734:1747	greater caecal concentrations	1719:1747	greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria	1719:1875	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	2	60	theme	meal-based	392:401	arg1	diets					403:407	either wheat- or corn-soybean meal-based diets	362:407	either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium or low sNSP content, resulting in 6 dietary treatments in a 2 × 3 factorial arrangement of treatments	362:553	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium or low sNSP content, resulting in 6 dietary treatments in a 2 × 3 factorial arrangement of treatments.
34198096	11	61	theme	beneficial	2321:2330	arg1	species					2342:2348	beneficial microbial species	2321:2348	beneficial microbial species	2321:2348	These results illustrate that dietary sNSP concentration, as well as its composition, have a direct impact on gastrointestinal viscosity and pH, and fuel beneficial microbial species, resulting in production of SCFA.
34198096	3	62	theme	caecal	699:704	arg1	profile					717:723	ileal and caecal microbiota profile	689:723	ileal and caecal microbiota profile	689:723	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
34198096	9	63	theme	acetic	1752:1757	arg1	acids					1790:1794	acetic, butyric, lactic, and succinic acids	1752:1794	acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively)	1752:1856	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	6	64	theme	higher	1074:1079	arg1	P = 0.010					1092:1100	P = 0.010	1092:1100	P = 0.010	1092:1100	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	6	64	theme	higher	1074:1079	arg1	pH					1088:1089	higher caecal pH	1074:1089	higher caecal pH (P = 0.010)	1074:1101	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	10	65	dep	P	2141:2141	arg1	<					2143:2143	<	2143:2143	<	2143:2143	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	6	66	theme	Lactobacillus	1229:1241	arg1	P = 0.043					1252:1260	P = 0.043	1252:1260	P = 0.043	1252:1260	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	6	66	theme	Lactobacillus	1229:1241	arg1	content					1243:1249	lower ileal Lactobacillus content	1217:1249	lower ileal Lactobacillus content (P = 0.043)	1217:1261	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	12	67	theme	wheat-based	2464:2474	arg1	diets					2476:2480	wheat-based diets	2464:2480	wheat-based diets for broilers	2464:2493	It appears to be particularly important to consider sNSP level when formulating wheat-based diets for broilers.
34198096	3	68	theme	ileum	616:620	arg1	viscosity					622:630	ileum viscosity	616:630	ileum viscosity	616:630	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
34198096	8	69	theme	wheat-based	1539:1549	arg1	diet					1551:1554	the wheat-based diet	1535:1554	the wheat-based diet with high sNSP level	1535:1575	At d 35, total caecal SCFA concentration was greater in birds fed the wheat-based diet with high sNSP level compared to those fed the corn-based diet with high or low sNSP level (P = 0.028).
34198096	2	70	theme	Cobb	301:304	arg1	broilers					310:317	Cobb 500 broilers	301:317	Cobb 500 broilers (n = 480, 80 birds per treatment)	301:351	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium or low sNSP content, resulting in 6 dietary treatments in a 2 × 3 factorial arrangement of treatments.
34198096	6	71	theme	lower	1217:1221	arg1	P = 0.043					1252:1260	P = 0.043	1252:1260	P = 0.043	1252:1260	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	6	71	theme	lower	1217:1221	arg1	content					1243:1249	lower ileal Lactobacillus content	1217:1249	lower ileal Lactobacillus content (P = 0.043)	1217:1261	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	3	72	theme	fatty	652:656	arg1	SCFA					664:667	SCFA	664:667	SCFA	664:667	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
34198096	3	72	theme	fatty	652:656	arg1	acid					658:661	caecal short chain fatty acid	633:661	caecal short chain fatty acid (SCFA) concentration	633:682	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
34198096	11	73	contain	have	2253:2256	arg1	composition					2240:2250	its composition	2236:2250	its composition	2236:2250	These results illustrate that dietary sNSP concentration, as well as its composition, have a direct impact on gastrointestinal viscosity and pH, and fuel beneficial microbial species, resulting in production of SCFA.
34198096	11	73	contain	have	2253:2256	arg1	concentration					2210:2222	dietary sNSP concentration	2197:2222	dietary sNSP concentration	2197:2222	These results illustrate that dietary sNSP concentration, as well as its composition, have a direct impact on gastrointestinal viscosity and pH, and fuel beneficial microbial species, resulting in production of SCFA.
34198096	11	73	contain	have	2253:2256	arg2	impact					2267:2272	a direct impact	2258:2272	a direct impact	2258:2272	These results illustrate that dietary sNSP concentration, as well as its composition, have a direct impact on gastrointestinal viscosity and pH, and fuel beneficial microbial species, resulting in production of SCFA.
34198096	3	74	theme	short	640:644	arg1	SCFA					664:667	SCFA	664:667	SCFA	664:667	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
34198096	3	74	theme	short	640:644	arg1	acid					658:661	caecal short chain fatty acid	633:661	caecal short chain fatty acid (SCFA) concentration	633:682	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
34198096	8	75	theme	sNSP	1566:1569	arg1	level					1571:1575	high sNSP level	1561:1575	high sNSP level	1561:1575	At d 35, total caecal SCFA concentration was greater in birds fed the wheat-based diet with high sNSP level compared to those fed the corn-based diet with high or low sNSP level (P = 0.028).
34198096	6	76	theme	high	1286:1289	arg1	sNSP					1291:1294	high sNSP	1286:1294	high sNSP	1286:1294	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	9	77	theme	acids	1790:1794	arg1	concentrations					1734:1747	greater caecal concentrations	1719:1747	greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria	1719:1875	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	2	78	theme	2 × 3	513:517	arg1	arrangement					529:539	a 2 × 3 factorial arrangement	511:539	a 2 × 3 factorial arrangement of treatments	511:553	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium or low sNSP content, resulting in 6 dietary treatments in a 2 × 3 factorial arrangement of treatments.
34198096	1	79	from	impact	251:256	arg1	environment					288:298	the gastrointestinal tract environment	261:298	the gastrointestinal tract environment	261:298	The aim of this study was to evaluate the importance of considering dietary soluble non-starch polysaccharides (sNSP) when formulating broiler diets, due to their impact on the gastrointestinal tract environment.
34198096	5	80	theme	P	923:923	arg1	viscosity					912:920	higher ileal viscosity	899:920	higher ileal viscosity (P < 0.001)	899:932	At both d 14 and d 35, birds fed the wheat-based diet presented higher ileal viscosity (P < 0.001) and lower ileal pH (P = 0.027 and P < 0.001, respectively) compared to those fed the corn-based diet.
34198096	5	80	theme	P	923:923	arg1	<					925:925	P < 0.001	923:931	P < 0.001	923:931	At both d 14 and d 35, birds fed the wheat-based diet presented higher ileal viscosity (P < 0.001) and lower ileal pH (P = 0.027 and P < 0.001, respectively) compared to those fed the corn-based diet.
34198096	1	81	theme	broiler	223:229	arg1	diets					231:235	broiler diets	223:235	broiler diets	223:235	The aim of this study was to evaluate the importance of considering dietary soluble non-starch polysaccharides (sNSP) when formulating broiler diets, due to their impact on the gastrointestinal tract environment.
34198096	10	82	from	d	2044:2044	arg1	acid					2009:2012	valeric acid	2001:2012	valeric acid at d14	2001:2019	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	10	82	from	d	2044:2044	arg1	Ruminococcus					1966:1977	Ruminococcus	1966:1977	Ruminococcus	1966:1977	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	10	82	from	d	2044:2044	arg1	acid					2036:2039	isobutyric acid	2025:2039	isobutyric acid at d 35	2025:2047	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	10	82	from	d	2044:2044	arg1	concentrations					1983:1996	concentrations	1983:1996	concentrations	1983:1996	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	11	83	theme	gastrointestinal	2277:2292	arg1	viscosity					2294:2302	gastrointestinal viscosity	2277:2302	gastrointestinal viscosity	2277:2302	These results illustrate that dietary sNSP concentration, as well as its composition, have a direct impact on gastrointestinal viscosity and pH, and fuel beneficial microbial species, resulting in production of SCFA.
34198096	9	84	theme	P	1808:1808	arg1	P = 0.001					1797:1805	P = 0.001	1797:1805	P = 0.001	1797:1805	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	9	84	theme	P	1808:1808	arg1	<					1810:1810	P < 0.001, P = 0.003 and P = 0.007	1808:1841	<	1810:1810	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	2	85	theme	treatments	544:553	arg1	arrangement					529:539	a 2 × 3 factorial arrangement	511:539	a 2 × 3 factorial arrangement of treatments	511:553	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium or low sNSP content, resulting in 6 dietary treatments in a 2 × 3 factorial arrangement of treatments.
34198096	10	86	from	Ruminococcus	1966:1977	arg1	d					2044:2044	d 35	2044:2047	d 35	2044:2047	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	10	86	from	Ruminococcus	1966:1977	arg1	d14					2017:2019	d14	2017:2019	d14	2017:2019	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	5	87	theme	higher	899:904	arg1	viscosity					912:920	higher ileal viscosity	899:920	higher ileal viscosity (P < 0.001)	899:932	At both d 14 and d 35, birds fed the wheat-based diet presented higher ileal viscosity (P < 0.001) and lower ileal pH (P = 0.027 and P < 0.001, respectively) compared to those fed the corn-based diet.
34198096	5	87	theme	higher	899:904	arg1	<					925:925	P < 0.001	923:931	P < 0.001	923:931	At both d 14 and d 35, birds fed the wheat-based diet presented higher ileal viscosity (P < 0.001) and lower ileal pH (P = 0.027 and P < 0.001, respectively) compared to those fed the corn-based diet.
34198096	3	88	theme	age	575:577	arg1	d					567:567	d 35	567:570	d 35	567:570	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
34198096	3	88	theme	age	575:577	arg1	d14					559:561	d14	559:561	d14	559:561	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
34198096	9	89	dep	birds	1677:1681	arg1	comparison					1663:1672	comparison	1663:1672	comparison	1663:1672	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	2	90	from	treatments	497:506	arg1	arrangement					529:539	a 2 × 3 factorial arrangement	511:539	a 2 × 3 factorial arrangement of treatments	511:553	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium or low sNSP content, resulting in 6 dietary treatments in a 2 × 3 factorial arrangement of treatments.
34198096	7	91	from	d	1409:1409	arg1	caeca					1400:1404	the caeca	1396:1404	the caeca at d 14	1396:1412	This effect was also seen for total SCFA (P = 0.017) and acetic acid (P = 0.005) concentrations in the caeca at d 14, but only in birds fed wheat-, not corn-, based diets.
34198096	8	92	theme	high	1624:1627	arg1	level					1641:1645	high or low sNSP level	1624:1645	high or low sNSP level (P = 0.028)	1624:1657	At d 35, total caecal SCFA concentration was greater in birds fed the wheat-based diet with high sNSP level compared to those fed the corn-based diet with high or low sNSP level (P = 0.028).
34198096	8	92	theme	high	1624:1627	arg1	P = 0.028					1648:1656	P = 0.028	1648:1656	P = 0.028	1648:1656	At d 35, total caecal SCFA concentration was greater in birds fed the wheat-based diet with high sNSP level compared to those fed the corn-based diet with high or low sNSP level (P = 0.028).
34198096	7	93	theme	wheat-	1437:1442	arg1	diets					1462:1466	wheat-, not corn-, based diets	1437:1466	wheat-, not corn-, based diets	1437:1466	This effect was also seen for total SCFA (P = 0.017) and acetic acid (P = 0.005) concentrations in the caeca at d 14, but only in birds fed wheat-, not corn-, based diets.
34198096	10	94	theme	caecal	1944:1949	arg1	populations					1951:1961	caecal populations	1944:1961	caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35	1944:2047	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	6	95	theme	valeric	1133:1139	arg1	P = 0.011					1187:1195	P = 0.011	1187:1195	P = 0.011	1187:1195	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	6	95	theme	valeric	1133:1139	arg1	P = 0.007					1173:1181	P = 0.007	1173:1181	P = 0.007	1173:1181	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	6	95	theme	valeric	1133:1139	arg1	concentrations					1146:1159	propionic, isobutyric and valeric acid concentrations	1107:1159	propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively)	1107:1210	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	8	96	theme	low	1632:1634	arg1	level					1641:1645	high or low sNSP level	1624:1645	high or low sNSP level (P = 0.028)	1624:1657	At d 35, total caecal SCFA concentration was greater in birds fed the wheat-based diet with high sNSP level compared to those fed the corn-based diet with high or low sNSP level (P = 0.028).
34198096	8	96	theme	low	1632:1634	arg1	P = 0.028					1648:1656	P = 0.028	1648:1656	P = 0.028	1648:1656	At d 35, total caecal SCFA concentration was greater in birds fed the wheat-based diet with high sNSP level compared to those fed the corn-based diet with high or low sNSP level (P = 0.028).
34198096	1	97	theme	soluble	164:170	arg1	sNSP					200:203	sNSP	200:203	sNSP	200:203	The aim of this study was to evaluate the importance of considering dietary soluble non-starch polysaccharides (sNSP) when formulating broiler diets, due to their impact on the gastrointestinal tract environment.
34198096	1	97	theme	soluble	164:170	arg1	polysaccharides					183:197	dietary soluble non-starch polysaccharides	156:197	dietary soluble non-starch polysaccharides (sNSP)	156:204	The aim of this study was to evaluate the importance of considering dietary soluble non-starch polysaccharides (sNSP) when formulating broiler diets, due to their impact on the gastrointestinal tract environment.
34198096	2	98	theme	sNSP	460:463	arg1	content					465:471	either a high, medium or low sNSP content	431:471	content	465:471	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium or low sNSP content, resulting in 6 dietary treatments in a 2 × 3 factorial arrangement of treatments.
34198096	10	99	theme	wheat-based	2097:2107	arg1	diets					2109:2113	wheat-based diets	2097:2113	wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively)	2097:2164	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	10	99	theme	wheat-based	2097:2107	arg1	P					2141:2141	P	2141:2141	P < 0.001, respectively	2141:2163	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	10	99	theme	wheat-based	2097:2107	arg1	P = 0.019					2127:2135	P = 0.019	2127:2135	P = 0.019	2127:2135	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	10	99	theme	wheat-based	2097:2107	arg1	P = 0.043					2116:2124	P = 0.043	2116:2124	P = 0.043	2116:2124	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	6	100	theme	acid	1141:1144	arg1	P = 0.011					1187:1195	P = 0.011	1187:1195	P = 0.011	1187:1195	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	6	100	theme	acid	1141:1144	arg1	P = 0.007					1173:1181	P = 0.007	1173:1181	P = 0.007	1173:1181	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	6	100	theme	acid	1141:1144	arg1	concentrations					1146:1159	propionic, isobutyric and valeric acid concentrations	1107:1159	propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively)	1107:1210	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	2	101	theme	dietary	489:495	arg1	treatments					497:506	6 dietary treatments	487:506	6 dietary treatments in a 2 × 3 factorial arrangement of treatments	487:553	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium or low sNSP content, resulting in 6 dietary treatments in a 2 × 3 factorial arrangement of treatments.
34198096	4	102	from	d	761:761	arg1	viscosity					748:756	Ileal viscosity	742:756	Ileal viscosity at d 35	742:764	Ileal viscosity at d 35 was greater in birds fed high sNSP compared to low sNSP (P = 0.022).
34198096	8	103	theme	caecal	1484:1489	arg1	concentration					1496:1508	total caecal SCFA concentration	1478:1508	total caecal SCFA concentration	1478:1508	At d 35, total caecal SCFA concentration was greater in birds fed the wheat-based diet with high sNSP level compared to those fed the corn-based diet with high or low sNSP level (P = 0.028).
34198096	5	104	dep	P	968:968	arg1	<					970:970	<	970:970	<	970:970	At both d 14 and d 35, birds fed the wheat-based diet presented higher ileal viscosity (P < 0.001) and lower ileal pH (P = 0.027 and P < 0.001, respectively) compared to those fed the corn-based diet.
34198096	0	105	theme	non-starch	8:17	arg1	polysaccharide					19:32	Soluble non-starch polysaccharide	0:32	Soluble non-starch polysaccharide	0:32	Soluble non-starch polysaccharide modulates broiler gastrointestinal tract environment.
34198096	5	106	theme	ileal	944:948	arg1	pH					950:951	lower ileal pH	938:951	lower ileal pH (P = 0.027 and P < 0.001, respectively)	938:991	At both d 14 and d 35, birds fed the wheat-based diet presented higher ileal viscosity (P < 0.001) and lower ileal pH (P = 0.027 and P < 0.001, respectively) compared to those fed the corn-based diet.
34198096	9	107	from	d14	1918:1920	arg1	d					1880:1880	d 35	1880:1883	d 35 (P = 0.003)	1880:1895	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	9	107	from	d14	1918:1920	arg1	P = 0.003					1886:1894	P = 0.003	1886:1894	P = 0.003	1886:1894	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	9	107	from	d14	1918:1920	arg1	acid					1910:1913	succinic acid	1901:1913	succinic acid at d14 (P = 0.041)	1901:1932	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	10	108	theme	acid	2009:2012	arg1	Ruminococcus					1966:1977	Ruminococcus	1966:1977	Ruminococcus	1966:1977	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	10	108	theme	acid	2009:2012	arg1	concentrations					1983:1996	concentrations	1983:1996	concentrations	1983:1996	However, caecal populations of Ruminococcus and concentrations of valeric acid at d14 and isobutyric acid at d 35 were greater in birds fed the corn- compared to wheat-based diets (P = 0.043, P = 0.019 and P < 0.001, respectively).
34198096	5	109	dep	pH	950:951	arg1	P					968:968	P	968:968	P < 0.001, respectively	968:990	At both d 14 and d 35, birds fed the wheat-based diet presented higher ileal viscosity (P < 0.001) and lower ileal pH (P = 0.027 and P < 0.001, respectively) compared to those fed the corn-based diet.
34198096	5	109	dep	pH	950:951	arg1	P = 0.027					954:962	P = 0.027	954:962	P = 0.027	954:962	At both d 14 and d 35, birds fed the wheat-based diet presented higher ileal viscosity (P < 0.001) and lower ileal pH (P = 0.027 and P < 0.001, respectively) compared to those fed the corn-based diet.
34198096	0	110	theme	gastrointestinal	52:67	arg1	environment					75:85	broiler gastrointestinal tract environment	44:85	broiler gastrointestinal tract environment	44:85	Soluble non-starch polysaccharide modulates broiler gastrointestinal tract environment.
34198096	9	111	theme	Bifidobacteria	1862:1875	arg1	concentrations					1734:1747	greater caecal concentrations	1719:1747	greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria	1719:1875	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	11	112	theme	dietary	2197:2203	arg1	concentration					2210:2222	dietary sNSP concentration	2197:2222	dietary sNSP concentration	2197:2222	These results illustrate that dietary sNSP concentration, as well as its composition, have a direct impact on gastrointestinal viscosity and pH, and fuel beneficial microbial species, resulting in production of SCFA.
34198096	6	113	theme	isobutyric	1118:1127	arg1	P = 0.011					1187:1195	P = 0.011	1187:1195	P = 0.011	1187:1195	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	6	113	theme	isobutyric	1118:1127	arg1	P = 0.007					1173:1181	P = 0.007	1173:1181	P = 0.007	1173:1181	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	6	113	theme	isobutyric	1118:1127	arg1	concentrations					1146:1159	propionic, isobutyric and valeric acid concentrations	1107:1159	propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively)	1107:1210	At d 14, birds fed low sNSP exhibited higher caecal pH (P = 0.010) and propionic, isobutyric and valeric acid concentrations (P = 0.035, P = 0.007 and P = 0.011, respectively), and lower ileal Lactobacillus content (P = 0.043), compared to birds fed high sNSP.
34198096	2	114	dep	content	465:471	arg1	either					431:436	either	431:436	either	431:436	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium or low sNSP content, resulting in 6 dietary treatments in a 2 × 3 factorial arrangement of treatments.
34198096	5	115	theme	wheat-based	872:882	arg1	diet					884:887	the wheat-based diet	868:887	the wheat-based diet	868:887	At both d 14 and d 35, birds fed the wheat-based diet presented higher ileal viscosity (P < 0.001) and lower ileal pH (P = 0.027 and P < 0.001, respectively) compared to those fed the corn-based diet.
34198096	9	116	theme	caecal	1727:1732	arg1	concentrations					1734:1747	greater caecal concentrations	1719:1747	greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria	1719:1875	In comparison to birds fed corn, birds fed wheat presented greater caecal concentrations of acetic, butyric, lactic, and succinic acids (P = 0.001, P < 0.001, P = 0.003 and P = 0.007, respectively) and Bifidobacteria at d 35 (P = 0.003) and succinic acid at d14 (P = 0.041).
34198096	12	117	theme	sNSP	2436:2439	arg1	level					2441:2445	sNSP level	2436:2445	sNSP level	2436:2445	It appears to be particularly important to consider sNSP level when formulating wheat-based diets for broilers.
34198096	1	118	theme	due	238:240	arg1	diets					231:235	broiler diets	223:235	broiler diets	223:235	The aim of this study was to evaluate the importance of considering dietary soluble non-starch polysaccharides (sNSP) when formulating broiler diets, due to their impact on the gastrointestinal tract environment.
34198096	11	119	theme	microbial	2332:2340	arg1	species					2342:2348	beneficial microbial species	2321:2348	beneficial microbial species	2321:2348	These results illustrate that dietary sNSP concentration, as well as its composition, have a direct impact on gastrointestinal viscosity and pH, and fuel beneficial microbial species, resulting in production of SCFA.
34198096	3	120	theme	ileal	689:693	arg1	profile					717:723	ileal and caecal microbiota profile	689:723	ileal and caecal microbiota profile	689:723	On d14 and d 35 of age, pH of the gizzard, ileum and caeca, ileum viscosity, caecal short chain fatty acid (SCFA) concentration, and ileal and caecal microbiota profile were determined.
33144260	3	0	theme	allergy-related	658:672	arg1	cytokines					674:682	the allergy-related cytokines	654:682	the allergy-related cytokines	654:682	After the administration of DESP, however, the anaphylactic symptoms (shortness of breath, hypothermia, diarrhea), along with the allergy-related cytokines, were effectively suppressed.
33144260	6	1	theme	Firmicutes	1042:1051	arg1	proportion					1028:1037	the proportion	1024:1037	the proportion of Firmicutes and Bacteroidetes/Proteobacteria	1024:1084	Taxonomic composition analysis showed that DESP modulated the proportion of Firmicutes and Bacteroidetes/Proteobacteria.
33144260	1	2	theme	depolymerized	160:172	arg1	polysaccharides					183:197	depolymerized sulfated polysaccharides	160:197	depolymerized sulfated polysaccharides from Eucheuma serra (DESP)	160:224	The ameliorative effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on ovalbumin (OVA)-caused induced food allergy was investigated in this work.
33144260	1	3	theme	-caused	244:250	arg1	allergy					265:271	ovalbumin (OVA)-caused induced food allergy	229:271	ovalbumin (OVA)-caused induced food allergy	229:271	The ameliorative effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on ovalbumin (OVA)-caused induced food allergy was investigated in this work.
33144260	2	4	theme	epithelial	453:462	arg1	damage					464:469	intestinal epithelial damage	442:469	intestinal epithelial damage	442:469	Results showed that OVA stimulated the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α) and led to diarrhea, intestinal epithelial damage, and intestinal microflora dysbiosis in sensitized mice.
33144260	7	5	dep	bacteria	1210:1217	arg1	bacteria					1210:1217	pathogenic bacteria	1199:1217	pathogenic bacteria (Helicobacteraceae and Desulfovibrionaceae)	1199:1261	Particularly, DESP increased probiotics (Lactobacillaceae, Bifidobacteriaceae and Prevotellaceae) and decreased pathogenic bacteria (Helicobacteraceae and Desulfovibrionaceae).
33144260	7	5	dep	bacteria	1210:1217	arg1	Desulfovibrionaceae					1242:1260	Desulfovibrionaceae	1242:1260	Desulfovibrionaceae	1242:1260	Particularly, DESP increased probiotics (Lactobacillaceae, Bifidobacteriaceae and Prevotellaceae) and decreased pathogenic bacteria (Helicobacteraceae and Desulfovibrionaceae).
33144260	7	5	dep	bacteria	1210:1217	arg1	Helicobacteraceae					1220:1236	Helicobacteraceae	1220:1236	Helicobacteraceae	1220:1236	Particularly, DESP increased probiotics (Lactobacillaceae, Bifidobacteriaceae and Prevotellaceae) and decreased pathogenic bacteria (Helicobacteraceae and Desulfovibrionaceae).
33144260	1	6	theme	sulfated	174:181	arg1	polysaccharides					183:197	depolymerized sulfated polysaccharides	160:197	depolymerized sulfated polysaccharides from Eucheuma serra (DESP)	160:224	The ameliorative effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on ovalbumin (OVA)-caused induced food allergy was investigated in this work.
33144260	1	7	theme	induced	252:258	arg1	allergy					265:271	ovalbumin (OVA)-caused induced food allergy	229:271	ovalbumin (OVA)-caused induced food allergy	229:271	The ameliorative effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on ovalbumin (OVA)-caused induced food allergy was investigated in this work.
33144260	0	8	theme	food	106:109	arg1	model					126:130	food allergic mouse model	106:130	food allergic mouse model	106:130	Depolymerized sulfated galactans from Eucheuma serra ameliorate allergic response and intestinal flora in food allergic mouse model.
33144260	4	9	theme	reduced	728:734	arg1	inflammation					747:758	the reduced intestinal inflammation	724:758	the reduced intestinal inflammation	724:758	Moreover, the reduced intestinal inflammation was discovered in the DESP-treated group.
33144260	1	10	theme	food	260:263	arg1	allergy					265:271	ovalbumin (OVA)-caused induced food allergy	229:271	ovalbumin (OVA)-caused induced food allergy	229:271	The ameliorative effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on ovalbumin (OVA)-caused induced food allergy was investigated in this work.
33144260	2	11	from	diarrhea	432:439	arg1	mice					522:525	sensitized mice	511:525	sensitized mice	511:525	Results showed that OVA stimulated the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α) and led to diarrhea, intestinal epithelial damage, and intestinal microflora dysbiosis in sensitized mice.
33144260	1	12	from	effect	150:155	arg1	DESP					220:223	DESP	220:223	DESP	220:223	The ameliorative effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on ovalbumin (OVA)-caused induced food allergy was investigated in this work.
33144260	1	12	from	effect	150:155	arg1	serra					213:217	Eucheuma serra	204:217	Eucheuma serra (DESP)	204:224	The ameliorative effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on ovalbumin (OVA)-caused induced food allergy was investigated in this work.
33144260	1	12	from	effect	150:155	arg1	allergy					265:271	ovalbumin (OVA)-caused induced food allergy	229:271	ovalbumin (OVA)-caused induced food allergy	229:271	The ameliorative effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on ovalbumin (OVA)-caused induced food allergy was investigated in this work.
33144260	5	13	theme	community	946:954	arg1	richness					956:963	microbial community richness	936:963	microbial community richness	936:963	Additionally, 16S rRNA sequencing of fecal samples was performed, and gene count and α-diversity analysis revealed that DESP improved microbial community richness.
33144260	6	14	theme	Taxonomic	966:974	arg1	analysis					988:995	Taxonomic composition analysis	966:995	Taxonomic composition analysis	966:995	Taxonomic composition analysis showed that DESP modulated the proportion of Firmicutes and Bacteroidetes/Proteobacteria.
33144260	2	15	theme	microflora	487:496	arg1	dysbiosis					498:506	intestinal microflora dysbiosis	476:506	intestinal microflora dysbiosis	476:506	Results showed that OVA stimulated the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α) and led to diarrhea, intestinal epithelial damage, and intestinal microflora dysbiosis in sensitized mice.
33144260	5	16	theme	16S	816:818	arg1	sequencing					825:834	16S rRNA sequencing	816:834	16S rRNA sequencing of fecal samples	816:851	Additionally, 16S rRNA sequencing of fecal samples was performed, and gene count and α-diversity analysis revealed that DESP improved microbial community richness.
33144260	2	17	dep	cytokines	372:380	arg1	IgE					396:398	OVA-specific IgE	383:398	OVA-specific IgE	383:398	Results showed that OVA stimulated the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α) and led to diarrhea, intestinal epithelial damage, and intestinal microflora dysbiosis in sensitized mice.
33144260	2	17	dep	cytokines	372:380	arg1	TNF-α					414:418	TNF-α	414:418	TNF-α	414:418	Results showed that OVA stimulated the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α) and led to diarrhea, intestinal epithelial damage, and intestinal microflora dysbiosis in sensitized mice.
33144260	2	17	dep	cytokines	372:380	arg1	IgA					409:411	IgA	409:411	IgA	409:411	Results showed that OVA stimulated the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α) and led to diarrhea, intestinal epithelial damage, and intestinal microflora dysbiosis in sensitized mice.
33144260	2	17	dep	cytokines	372:380	arg1	mMCP-1					401:406	mMCP-1	401:406	mMCP-1	401:406	Results showed that OVA stimulated the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α) and led to diarrhea, intestinal epithelial damage, and intestinal microflora dysbiosis in sensitized mice.
33144260	2	18	theme	OVA-specific	383:394	arg1	IgE					396:398	OVA-specific IgE	383:398	OVA-specific IgE	383:398	Results showed that OVA stimulated the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α) and led to diarrhea, intestinal epithelial damage, and intestinal microflora dysbiosis in sensitized mice.
33144260	2	18	theme	OVA-specific	383:394	arg1	TNF-α					414:418	TNF-α	414:418	TNF-α	414:418	Results showed that OVA stimulated the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α) and led to diarrhea, intestinal epithelial damage, and intestinal microflora dysbiosis in sensitized mice.
33144260	2	18	theme	OVA-specific	383:394	arg1	IgA					409:411	IgA	409:411	IgA	409:411	Results showed that OVA stimulated the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α) and led to diarrhea, intestinal epithelial damage, and intestinal microflora dysbiosis in sensitized mice.
33144260	2	18	theme	OVA-specific	383:394	arg1	mMCP-1					401:406	mMCP-1	401:406	mMCP-1	401:406	Results showed that OVA stimulated the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α) and led to diarrhea, intestinal epithelial damage, and intestinal microflora dysbiosis in sensitized mice.
33144260	1	19	theme	polysaccharides	183:197	arg1	effect					150:155	The ameliorative effect	133:155	The ameliorative effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on ovalbumin (OVA)-caused induced food allergy	133:271	The ameliorative effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on ovalbumin (OVA)-caused induced food allergy was investigated in this work.
33144260	0	20	theme	allergic	111:118	arg1	model					126:130	food allergic mouse model	106:130	food allergic mouse model	106:130	Depolymerized sulfated galactans from Eucheuma serra ameliorate allergic response and intestinal flora in food allergic mouse model.
33144260	0	21	from	serra	47:51	arg1	galactans					23:31	Depolymerized sulfated galactans	0:31	Depolymerized sulfated galactans from Eucheuma serra	0:51	Depolymerized sulfated galactans from Eucheuma serra ameliorate allergic response and intestinal flora in food allergic mouse model.
33144260	4	22	theme	intestinal	736:745	arg1	inflammation					747:758	the reduced intestinal inflammation	724:758	the reduced intestinal inflammation	724:758	Moreover, the reduced intestinal inflammation was discovered in the DESP-treated group.
33144260	7	23	dep	probiotics	1116:1125	arg1	probiotics					1116:1125	probiotics	1116:1125	probiotics (Lactobacillaceae, Bifidobacteriaceae and Prevotellaceae)	1116:1183	Particularly, DESP increased probiotics (Lactobacillaceae, Bifidobacteriaceae and Prevotellaceae) and decreased pathogenic bacteria (Helicobacteraceae and Desulfovibrionaceae).
33144260	7	23	dep	probiotics	1116:1125	arg1	Prevotellaceae					1169:1182	Prevotellaceae	1169:1182	Prevotellaceae	1169:1182	Particularly, DESP increased probiotics (Lactobacillaceae, Bifidobacteriaceae and Prevotellaceae) and decreased pathogenic bacteria (Helicobacteraceae and Desulfovibrionaceae).
33144260	7	23	dep	probiotics	1116:1125	arg1	Bifidobacteriaceae					1146:1163	Bifidobacteriaceae	1146:1163	Bifidobacteriaceae	1146:1163	Particularly, DESP increased probiotics (Lactobacillaceae, Bifidobacteriaceae and Prevotellaceae) and decreased pathogenic bacteria (Helicobacteraceae and Desulfovibrionaceae).
33144260	7	23	dep	probiotics	1116:1125	arg1	Lactobacillaceae					1128:1143	Lactobacillaceae	1128:1143	Lactobacillaceae	1128:1143	Particularly, DESP increased probiotics (Lactobacillaceae, Bifidobacteriaceae and Prevotellaceae) and decreased pathogenic bacteria (Helicobacteraceae and Desulfovibrionaceae).
33144260	0	24	theme	mouse	120:124	arg1	model					126:130	food allergic mouse model	106:130	food allergic mouse model	106:130	Depolymerized sulfated galactans from Eucheuma serra ameliorate allergic response and intestinal flora in food allergic mouse model.
33144260	0	25	theme	sulfated	14:21	arg1	galactans					23:31	Depolymerized sulfated galactans	0:31	Depolymerized sulfated galactans from Eucheuma serra	0:51	Depolymerized sulfated galactans from Eucheuma serra ameliorate allergic response and intestinal flora in food allergic mouse model.
33144260	0	26	theme	intestinal	86:95	arg1	flora					97:101	intestinal flora	86:101	intestinal flora	86:101	Depolymerized sulfated galactans from Eucheuma serra ameliorate allergic response and intestinal flora in food allergic mouse model.
33144260	1	27	theme	Eucheuma	204:211	arg1	DESP					220:223	DESP	220:223	DESP	220:223	The ameliorative effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on ovalbumin (OVA)-caused induced food allergy was investigated in this work.
33144260	1	27	theme	Eucheuma	204:211	arg1	serra					213:217	Eucheuma serra	204:217	Eucheuma serra (DESP)	204:224	The ameliorative effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on ovalbumin (OVA)-caused induced food allergy was investigated in this work.
33144260	0	28	theme	Depolymerized	0:12	arg1	galactans					23:31	Depolymerized sulfated galactans	0:31	Depolymerized sulfated galactans from Eucheuma serra	0:51	Depolymerized sulfated galactans from Eucheuma serra ameliorate allergic response and intestinal flora in food allergic mouse model.
33144260	3	29	dep	along	643:647	arg1	with					649:652	with	649:652	with	649:652	After the administration of DESP, however, the anaphylactic symptoms (shortness of breath, hypothermia, diarrhea), along with the allergy-related cytokines, were effectively suppressed.
33144260	3	30	theme	DESP	556:559	arg1	administration					538:551	the administration	534:551	the administration of DESP	534:559	After the administration of DESP, however, the anaphylactic symptoms (shortness of breath, hypothermia, diarrhea), along with the allergy-related cytokines, were effectively suppressed.
33144260	6	31	theme	Bacteroidetes/Proteobacteria	1057:1084	arg1	proportion					1028:1037	the proportion	1024:1037	the proportion of Firmicutes and Bacteroidetes/Proteobacteria	1024:1084	Taxonomic composition analysis showed that DESP modulated the proportion of Firmicutes and Bacteroidetes/Proteobacteria.
33144260	7	32	theme	pathogenic	1199:1208	arg1	bacteria					1210:1217	pathogenic bacteria	1199:1217	pathogenic bacteria (Helicobacteraceae and Desulfovibrionaceae)	1199:1261	Particularly, DESP increased probiotics (Lactobacillaceae, Bifidobacteriaceae and Prevotellaceae) and decreased pathogenic bacteria (Helicobacteraceae and Desulfovibrionaceae).
33144260	7	32	theme	pathogenic	1199:1208	arg1	Desulfovibrionaceae					1242:1260	Desulfovibrionaceae	1242:1260	Desulfovibrionaceae	1242:1260	Particularly, DESP increased probiotics (Lactobacillaceae, Bifidobacteriaceae and Prevotellaceae) and decreased pathogenic bacteria (Helicobacteraceae and Desulfovibrionaceae).
33144260	7	32	theme	pathogenic	1199:1208	arg1	Helicobacteraceae					1220:1236	Helicobacteraceae	1220:1236	Helicobacteraceae	1220:1236	Particularly, DESP increased probiotics (Lactobacillaceae, Bifidobacteriaceae and Prevotellaceae) and decreased pathogenic bacteria (Helicobacteraceae and Desulfovibrionaceae).
33144260	2	33	from	damage	464:469	arg1	mice					522:525	sensitized mice	511:525	sensitized mice	511:525	Results showed that OVA stimulated the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α) and led to diarrhea, intestinal epithelial damage, and intestinal microflora dysbiosis in sensitized mice.
33144260	5	34	theme	gene	872:875	arg1	count					877:881	gene count	872:881	gene count	872:881	Additionally, 16S rRNA sequencing of fecal samples was performed, and gene count and α-diversity analysis revealed that DESP improved microbial community richness.
33144260	3	35	dep	symptoms	588:595	arg1	diarrhea					632:639	diarrhea	632:639	diarrhea	632:639	After the administration of DESP, however, the anaphylactic symptoms (shortness of breath, hypothermia, diarrhea), along with the allergy-related cytokines, were effectively suppressed.
33144260	3	35	dep	symptoms	588:595	arg1	hypothermia					619:629	hypothermia	619:629	hypothermia	619:629	After the administration of DESP, however, the anaphylactic symptoms (shortness of breath, hypothermia, diarrhea), along with the allergy-related cytokines, were effectively suppressed.
33144260	3	35	dep	symptoms	588:595	arg1	shortness					598:606	shortness	598:606	shortness of breath	598:616	After the administration of DESP, however, the anaphylactic symptoms (shortness of breath, hypothermia, diarrhea), along with the allergy-related cytokines, were effectively suppressed.
33144260	2	36	theme	intestinal	442:451	arg1	damage					464:469	intestinal epithelial damage	442:469	intestinal epithelial damage	442:469	Results showed that OVA stimulated the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α) and led to diarrhea, intestinal epithelial damage, and intestinal microflora dysbiosis in sensitized mice.
33144260	8	37	theme	microbiota	1374:1383	arg1	regulation					1349:1358	the regulation	1345:1358	the regulation of intestinal microbiota	1345:1383	These findings, therefore, suggest that DESP may ameliorate food allergy through the regulation of intestinal microbiota.
33144260	5	38	theme	rRNA	820:823	arg1	sequencing					825:834	16S rRNA sequencing	816:834	16S rRNA sequencing of fecal samples	816:851	Additionally, 16S rRNA sequencing of fecal samples was performed, and gene count and α-diversity analysis revealed that DESP improved microbial community richness.
33144260	2	39	theme	sensitized	511:520	arg1	mice					522:525	sensitized mice	511:525	sensitized mice	511:525	Results showed that OVA stimulated the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α) and led to diarrhea, intestinal epithelial damage, and intestinal microflora dysbiosis in sensitized mice.
33144260	4	40	theme	DESP-treated	782:793	arg1	group					795:799	the DESP-treated group	778:799	the DESP-treated group	778:799	Moreover, the reduced intestinal inflammation was discovered in the DESP-treated group.
33144260	2	41	theme	intestinal	476:485	arg1	microflora					487:496	intestinal microflora	476:496	intestinal microflora dysbiosis	476:506	Results showed that OVA stimulated the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α) and led to diarrhea, intestinal epithelial damage, and intestinal microflora dysbiosis in sensitized mice.
33144260	0	42	theme	allergic	64:71	arg1	response					73:80	allergic response	64:80	allergic response	64:80	Depolymerized sulfated galactans from Eucheuma serra ameliorate allergic response and intestinal flora in food allergic mouse model.
33144260	5	43	theme	samples	845:851	arg1	sequencing					825:834	16S rRNA sequencing	816:834	16S rRNA sequencing of fecal samples	816:851	Additionally, 16S rRNA sequencing of fecal samples was performed, and gene count and α-diversity analysis revealed that DESP improved microbial community richness.
33144260	3	44	theme	breath	611:616	arg1	diarrhea					632:639	diarrhea	632:639	diarrhea	632:639	After the administration of DESP, however, the anaphylactic symptoms (shortness of breath, hypothermia, diarrhea), along with the allergy-related cytokines, were effectively suppressed.
33144260	3	44	theme	breath	611:616	arg1	hypothermia					619:629	hypothermia	619:629	hypothermia	619:629	After the administration of DESP, however, the anaphylactic symptoms (shortness of breath, hypothermia, diarrhea), along with the allergy-related cytokines, were effectively suppressed.
33144260	3	44	theme	breath	611:616	arg1	shortness					598:606	shortness	598:606	shortness of breath	598:616	After the administration of DESP, however, the anaphylactic symptoms (shortness of breath, hypothermia, diarrhea), along with the allergy-related cytokines, were effectively suppressed.
33144260	8	45	theme	intestinal	1363:1372	arg1	microbiota					1374:1383	intestinal microbiota	1363:1383	intestinal microbiota	1363:1383	These findings, therefore, suggest that DESP may ameliorate food allergy through the regulation of intestinal microbiota.
33144260	5	46	theme	α-diversity	887:897	arg1	analysis					899:906	α-diversity analysis	887:906	α-diversity analysis	887:906	Additionally, 16S rRNA sequencing of fecal samples was performed, and gene count and α-diversity analysis revealed that DESP improved microbial community richness.
33144260	8	47	theme	food	1324:1327	arg1	allergy					1329:1335	food allergy	1324:1335	food allergy	1324:1335	These findings, therefore, suggest that DESP may ameliorate food allergy through the regulation of intestinal microbiota.
33144260	1	48	theme	ovalbumin	229:237	arg1	allergy					265:271	ovalbumin (OVA)-caused induced food allergy	229:271	ovalbumin (OVA)-caused induced food allergy	229:271	The ameliorative effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on ovalbumin (OVA)-caused induced food allergy was investigated in this work.
33144260	5	49	theme	microbial	936:944	arg1	richness					956:963	microbial community richness	936:963	microbial community richness	936:963	Additionally, 16S rRNA sequencing of fecal samples was performed, and gene count and α-diversity analysis revealed that DESP improved microbial community richness.
33144260	1	50	theme	ameliorative	137:148	arg1	effect					150:155	The ameliorative effect	133:155	The ameliorative effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on ovalbumin (OVA)-caused induced food allergy	133:271	The ameliorative effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on ovalbumin (OVA)-caused induced food allergy was investigated in this work.
33144260	2	51	from	dysbiosis	498:506	arg1	mice					522:525	sensitized mice	511:525	sensitized mice	511:525	Results showed that OVA stimulated the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α) and led to diarrhea, intestinal epithelial damage, and intestinal microflora dysbiosis in sensitized mice.
33144260	3	52	theme	anaphylactic	575:586	arg1	symptoms					588:595	the anaphylactic symptoms	571:595	the anaphylactic symptoms (shortness of breath, hypothermia, diarrhea)	571:640	After the administration of DESP, however, the anaphylactic symptoms (shortness of breath, hypothermia, diarrhea), along with the allergy-related cytokines, were effectively suppressed.
33144260	1	53	from	serra	213:217	arg1	polysaccharides					183:197	depolymerized sulfated polysaccharides	160:197	depolymerized sulfated polysaccharides from Eucheuma serra (DESP)	160:224	The ameliorative effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on ovalbumin (OVA)-caused induced food allergy was investigated in this work.
33144260	1	53	from	serra	213:217	arg1	effect					150:155	The ameliorative effect	133:155	The ameliorative effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on ovalbumin (OVA)-caused induced food allergy	133:271	The ameliorative effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on ovalbumin (OVA)-caused induced food allergy was investigated in this work.
33144260	2	54	theme	cytokines	372:380	arg1	secretion					343:351	the secretion	339:351	the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α)	339:419	Results showed that OVA stimulated the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α) and led to diarrhea, intestinal epithelial damage, and intestinal microflora dysbiosis in sensitized mice.
33144260	5	55	theme	fecal	839:843	arg1	samples					845:851	fecal samples	839:851	fecal samples	839:851	Additionally, 16S rRNA sequencing of fecal samples was performed, and gene count and α-diversity analysis revealed that DESP improved microbial community richness.
33144260	6	56	theme	composition	976:986	arg1	analysis					988:995	Taxonomic composition analysis	966:995	Taxonomic composition analysis	966:995	Taxonomic composition analysis showed that DESP modulated the proportion of Firmicutes and Bacteroidetes/Proteobacteria.
33144260	2	57	theme	allergy-related	356:370	arg1	cytokines					372:380	allergy-related cytokines	356:380	allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α)	356:419	Results showed that OVA stimulated the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α) and led to diarrhea, intestinal epithelial damage, and intestinal microflora dysbiosis in sensitized mice.
33161092	4	0	theme	protein	859:865	arg1	carbonyl					867:874	protein carbonyl	859:874	protein carbonyl	859:874	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	5	1	theme	phagocytic	979:988	arg1	activities					990:999	phagocytic activities	979:999	phagocytic activities	979:999	The results indicated that G3 (0.5 g kg -1 APS +0.5 g kg -1 CGA) showed higher total hemocyte counts, phagocytic activities, antibacterial activities and bacteriolytic activities during 6 days feeding, and without biomolecule damages after 6 days post-withdrawal.
33161092	6	2	theme	immunostimulants	1168:1183	arg1	formula					1185:1191	the appropriate immunostimulants formula	1152:1191	the appropriate immunostimulants formula in this study	1152:1205	Therefore, the appropriate immunostimulants formula in this study was the combination of 0.5 g kg -1 APS and 0.5 g kg-1 CGA, which was used for 6 days followed by 6 days post-withdrawal.
33161092	6	2	theme	immunostimulants	1168:1183	arg1	combination					1215:1225	the combination	1211:1225	the combination of 0.5 g kg -1 APS and 0.5 g kg-1 CGA	1211:1263	Therefore, the appropriate immunostimulants formula in this study was the combination of 0.5 g kg -1 APS and 0.5 g kg-1 CGA, which was used for 6 days followed by 6 days post-withdrawal.
33161092	5	3	theme	post-withdrawal	1124:1138	arg1	days					1119:1122	6 days post-withdrawal	1117:1138	6 days post-withdrawal	1117:1138	The results indicated that G3 (0.5 g kg -1 APS +0.5 g kg -1 CGA) showed higher total hemocyte counts, phagocytic activities, antibacterial activities and bacteriolytic activities during 6 days feeding, and without biomolecule damages after 6 days post-withdrawal.
33161092	1	4	theme	berberine	189:197	arg1	effects					118:124	In vitro and in vivo effects	97:124	In vitro and in vivo effects of Astragalus polysaccharide (APS), chlorogenic acid (CGA) and berberine (BBR) on shrimp (Litopenaeus vannamei)	97:236	In vitro and in vivo effects of Astragalus polysaccharide (APS), chlorogenic acid (CGA) and berberine (BBR) on shrimp (Litopenaeus vannamei) were studied.
33161092	2	5	theme	BBR	305:307	arg1	combination					317:327	the combination	313:327	the combination of APS and CGA	313:342	In vitro test showed that the combination of APS and BBR and the combination of APS and CGA have strong immune enhancement effects and no lysosomal membrane damage on hemocyte.
33161092	2	5	theme	BBR	305:307	arg1	combination					282:292	the combination	278:292	the combination of APS and BBR	278:307	In vitro test showed that the combination of APS and BBR and the combination of APS and CGA have strong immune enhancement effects and no lysosomal membrane damage on hemocyte.
33161092	4	6	theme	common	729:734	arg1	group					722:726	the control group	710:726	the control group (common diet)	710:740	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	6	theme	common	729:734	arg1	diet					736:739	common diet	729:739	common diet	729:739	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	1	7	from	effects	118:124	arg1	shrimp					208:213	shrimp	208:213	shrimp (Litopenaeus vannamei)	208:236	In vitro and in vivo effects of Astragalus polysaccharide (APS), chlorogenic acid (CGA) and berberine (BBR) on shrimp (Litopenaeus vannamei) were studied.
33161092	4	8	contain	containing	539:548	arg1	diets					533:537	Four diets	528:537	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet)	528:740	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	8	contain	containing	539:548	arg2	G1-G4					550:554	G1-G4	550:554	G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet)	550:740	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	9	theme	0.5 g kg	625:632	arg1	BBR					586:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	9	theme	0.5 g kg	625:632	arg1	CGA					654:656	0.5 g kg -1 APS +0.5 g kg -1 CGA	625:656	0.5 g kg -1 APS +0.5 g kg -1 CGA	625:656	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	10	theme	DNA	824:826	arg1	damage					828:833	DNA damage	824:833	DNA damage	824:833	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	1	11	dep	shrimp	208:213	arg1	vannamei					228:235	Litopenaeus vannamei	216:235	Litopenaeus vannamei	216:235	In vitro and in vivo effects of Astragalus polysaccharide (APS), chlorogenic acid (CGA) and berberine (BBR) on shrimp (Litopenaeus vannamei) were studied.
33161092	3	12	theme	feeding	435:441	arg1	experiment					443:452	feeding experiment	435:452	feeding experiment	435:452	Then, feeding experiment was proceeded to optimize the concentrations of compound herbal extracts.
33161092	6	13	theme	days	1306:1309	arg1	post-withdrawal					1311:1325	6 days post-withdrawal	1304:1325	6 days post-withdrawal	1304:1325	Therefore, the appropriate immunostimulants formula in this study was the combination of 0.5 g kg -1 APS and 0.5 g kg-1 CGA, which was used for 6 days followed by 6 days post-withdrawal.
33161092	2	14	dep	In	252:253	arg1	vitro					255:259	vitro	255:259	vitro	255:259	In vitro test showed that the combination of APS and BBR and the combination of APS and CGA have strong immune enhancement effects and no lysosomal membrane damage on hemocyte.
33161092	5	15	theme	higher	949:954	arg1	counts					971:976	higher total hemocyte counts	949:976	higher total hemocyte counts	949:976	The results indicated that G3 (0.5 g kg -1 APS +0.5 g kg -1 CGA) showed higher total hemocyte counts, phagocytic activities, antibacterial activities and bacteriolytic activities during 6 days feeding, and without biomolecule damages after 6 days post-withdrawal.
33161092	5	16	theme	bacteriolytic	1031:1043	arg1	activities					1045:1054	bacteriolytic activities	1031:1054	bacteriolytic activities	1031:1054	The results indicated that G3 (0.5 g kg -1 APS +0.5 g kg -1 CGA) showed higher total hemocyte counts, phagocytic activities, antibacterial activities and bacteriolytic activities during 6 days feeding, and without biomolecule damages after 6 days post-withdrawal.
33161092	4	17	theme	+0.5 g kg	641:649	arg1	BBR					586:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	17	theme	+0.5 g kg	641:649	arg1	CGA					654:656	0.5 g kg -1 APS +0.5 g kg -1 CGA	625:656	0.5 g kg -1 APS +0.5 g kg -1 CGA	625:656	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	7	18	theme	primary	1431:1437	arg1	culture					1455:1461	primary shrimp hemocyte culture	1431:1461	primary shrimp hemocyte culture	1431:1461	Additionally, our study provides new support for screening composite immunostimulants formula by using primary shrimp hemocyte culture.
33161092	0	19	from	potentiation	33:44	arg1	shrimp					66:71	shrimp	66:71	shrimp (Litopenaeus vannamei)	66:94	In vitro and in vivo immunologic potentiation of herb extracts on shrimp (Litopenaeus vannamei).
33161092	4	20	theme	control	714:720	arg1	group					722:726	the control group	710:726	the control group (common diet)	710:740	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	20	theme	control	714:720	arg1	diet					736:739	common diet	729:739	common diet	729:739	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	2	21	contain	have	344:347	arg2	effects					375:381	strong immune enhancement effects	349:381	strong immune enhancement effects	349:381	In vitro test showed that the combination of APS and BBR and the combination of APS and CGA have strong immune enhancement effects and no lysosomal membrane damage on hemocyte.
33161092	2	21	contain	have	344:347	arg1	combination					317:327	the combination	313:327	the combination of APS and CGA	313:342	In vitro test showed that the combination of APS and BBR and the combination of APS and CGA have strong immune enhancement effects and no lysosomal membrane damage on hemocyte.
33161092	2	21	contain	have	344:347	arg2	damage					409:414	no lysosomal membrane damage	387:414	no lysosomal membrane damage	387:414	In vitro test showed that the combination of APS and BBR and the combination of APS and CGA have strong immune enhancement effects and no lysosomal membrane damage on hemocyte.
33161092	2	21	contain	have	344:347	arg1	combination					282:292	the combination	278:292	the combination of APS and BBR	278:307	In vitro test showed that the combination of APS and BBR and the combination of APS and CGA have strong immune enhancement effects and no lysosomal membrane damage on hemocyte.
33161092	1	22	theme	Astragalus	129:138	arg1	APS					156:158	APS	156:158	APS	156:158	In vitro and in vivo effects of Astragalus polysaccharide (APS), chlorogenic acid (CGA) and berberine (BBR) on shrimp (Litopenaeus vannamei) were studied.
33161092	1	22	theme	Astragalus	129:138	arg1	polysaccharide					140:153	Astragalus polysaccharide	129:153	Astragalus polysaccharide (APS)	129:159	In vitro and in vivo effects of Astragalus polysaccharide (APS), chlorogenic acid (CGA) and berberine (BBR) on shrimp (Litopenaeus vannamei) were studied.
33161092	6	23	theme	0.5 g kg	1230:1237	arg1	APS					1242:1244	0.5 g kg -1 APS	1230:1244	0.5 g kg -1 APS	1230:1244	Therefore, the appropriate immunostimulants formula in this study was the combination of 0.5 g kg -1 APS and 0.5 g kg-1 CGA, which was used for 6 days followed by 6 days post-withdrawal.
33161092	5	24	theme	hemocyte	962:969	arg1	counts					971:976	higher total hemocyte counts	949:976	higher total hemocyte counts	949:976	The results indicated that G3 (0.5 g kg -1 APS +0.5 g kg -1 CGA) showed higher total hemocyte counts, phagocytic activities, antibacterial activities and bacteriolytic activities during 6 days feeding, and without biomolecule damages after 6 days post-withdrawal.
33161092	7	25	theme	shrimp	1439:1444	arg1	culture					1455:1461	primary shrimp hemocyte culture	1431:1461	primary shrimp hemocyte culture	1431:1461	Additionally, our study provides new support for screening composite immunostimulants formula by using primary shrimp hemocyte culture.
33161092	1	26	dep	In	97:98	arg1	vitro					100:104	vitro	100:104	vitro	100:104	In vitro and in vivo effects of Astragalus polysaccharide (APS), chlorogenic acid (CGA) and berberine (BBR) on shrimp (Litopenaeus vannamei) were studied.
33161092	1	27	theme	polysaccharide	140:153	arg1	effects					118:124	In vitro and in vivo effects	97:124	In vitro and in vivo effects of Astragalus polysaccharide (APS), chlorogenic acid (CGA) and berberine (BBR) on shrimp (Litopenaeus vannamei)	97:236	In vitro and in vivo effects of Astragalus polysaccharide (APS), chlorogenic acid (CGA) and berberine (BBR) on shrimp (Litopenaeus vannamei) were studied.
33161092	5	28	theme	days	1065:1068	arg1	feeding					1070:1076	6 days feeding	1063:1076	6 days feeding	1063:1076	The results indicated that G3 (0.5 g kg -1 APS +0.5 g kg -1 CGA) showed higher total hemocyte counts, phagocytic activities, antibacterial activities and bacteriolytic activities during 6 days feeding, and without biomolecule damages after 6 days post-withdrawal.
33161092	2	29	theme	membrane	400:407	arg1	damage					409:414	no lysosomal membrane damage	387:414	no lysosomal membrane damage	387:414	In vitro test showed that the combination of APS and BBR and the combination of APS and CGA have strong immune enhancement effects and no lysosomal membrane damage on hemocyte.
33161092	0	30	dep	shrimp	66:71	arg1	vannamei					86:93	Litopenaeus vannamei	74:93	Litopenaeus vannamei	74:93	In vitro and in vivo immunologic potentiation of herb extracts on shrimp (Litopenaeus vannamei).
33161092	5	31	theme	total	956:960	arg1	counts					971:976	higher total hemocyte counts	949:976	higher total hemocyte counts	949:976	The results indicated that G3 (0.5 g kg -1 APS +0.5 g kg -1 CGA) showed higher total hemocyte counts, phagocytic activities, antibacterial activities and bacteriolytic activities during 6 days feeding, and without biomolecule damages after 6 days post-withdrawal.
33161092	2	32	theme	APS	297:299	arg1	combination					317:327	the combination	313:327	the combination of APS and CGA	313:342	In vitro test showed that the combination of APS and BBR and the combination of APS and CGA have strong immune enhancement effects and no lysosomal membrane damage on hemocyte.
33161092	2	32	theme	APS	297:299	arg1	combination					282:292	the combination	278:292	the combination of APS and BBR	278:307	In vitro test showed that the combination of APS and BBR and the combination of APS and CGA have strong immune enhancement effects and no lysosomal membrane damage on hemocyte.
33161092	0	33	theme	In	0:1	arg1	potentiation					33:44	In vitro and in vivo immunologic potentiation	0:44	In vitro and in vivo immunologic potentiation of herb extracts on shrimp (Litopenaeus vannamei).	0:95	In vitro and in vivo immunologic potentiation of herb extracts on shrimp (Litopenaeus vannamei).
33161092	4	34	theme	APS + 0.5 g kg	568:581	arg1	BBR					586:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	34	theme	APS + 0.5 g kg	568:581	arg1	CGA					654:656	0.5 g kg -1 APS +0.5 g kg -1 CGA	625:656	0.5 g kg -1 APS +0.5 g kg -1 CGA	625:656	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	34	theme	APS + 0.5 g kg	568:581	arg1	CGA					689:691	1.0 g kg -1 APS + 1.0 g kg -1 CGA	659:691	1.0 g kg -1 APS + 1.0 g kg -1 CGA	659:691	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	34	theme	APS + 0.5 g kg	568:581	arg1	BBR					620:622	1.0 g kg -1 APS +1.0 g kg -1 BBR	591:622	1.0 g kg -1 APS +1.0 g kg -1 BBR	591:622	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	0	35	theme	in	13:14	arg1	potentiation					33:44	In vitro and in vivo immunologic potentiation	0:44	In vitro and in vivo immunologic potentiation of herb extracts on shrimp (Litopenaeus vannamei).	0:95	In vitro and in vivo immunologic potentiation of herb extracts on shrimp (Litopenaeus vannamei).
33161092	6	36	theme	CGA	1261:1263	arg1	formula					1185:1191	the appropriate immunostimulants formula	1152:1191	the appropriate immunostimulants formula in this study	1152:1205	Therefore, the appropriate immunostimulants formula in this study was the combination of 0.5 g kg -1 APS and 0.5 g kg-1 CGA, which was used for 6 days followed by 6 days post-withdrawal.
33161092	6	36	theme	CGA	1261:1263	arg1	combination					1215:1225	the combination	1211:1225	the combination of 0.5 g kg -1 APS and 0.5 g kg-1 CGA	1211:1263	Therefore, the appropriate immunostimulants formula in this study was the combination of 0.5 g kg -1 APS and 0.5 g kg-1 CGA, which was used for 6 days followed by 6 days post-withdrawal.
33161092	1	37	dep	in	110:111	arg1	vivo					113:116	vivo	113:116	vivo	113:116	In vitro and in vivo effects of Astragalus polysaccharide (APS), chlorogenic acid (CGA) and berberine (BBR) on shrimp (Litopenaeus vannamei) were studied.
33161092	7	38	theme	immunostimulants	1397:1412	arg1	formula					1414:1420	composite immunostimulants formula	1387:1420	composite immunostimulants formula	1387:1420	Additionally, our study provides new support for screening composite immunostimulants formula by using primary shrimp hemocyte culture.
33161092	2	39	theme	APS	332:334	arg1	combination					317:327	the combination	313:327	the combination of APS and CGA	313:342	In vitro test showed that the combination of APS and BBR and the combination of APS and CGA have strong immune enhancement effects and no lysosomal membrane damage on hemocyte.
33161092	2	39	theme	APS	332:334	arg1	combination					282:292	the combination	278:292	the combination of APS and BBR	278:307	In vitro test showed that the combination of APS and BBR and the combination of APS and CGA have strong immune enhancement effects and no lysosomal membrane damage on hemocyte.
33161092	2	40	theme	CGA	340:342	arg1	combination					317:327	the combination	313:327	the combination of APS and CGA	313:342	In vitro test showed that the combination of APS and BBR and the combination of APS and CGA have strong immune enhancement effects and no lysosomal membrane damage on hemocyte.
33161092	2	40	theme	CGA	340:342	arg1	combination					282:292	the combination	278:292	the combination of APS and BBR	278:307	In vitro test showed that the combination of APS and BBR and the combination of APS and CGA have strong immune enhancement effects and no lysosomal membrane damage on hemocyte.
33161092	4	41	theme	1.0 g kg	591:598	arg1	BBR					586:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	41	theme	1.0 g kg	591:598	arg1	BBR					620:622	1.0 g kg -1 APS +1.0 g kg -1 BBR	591:622	1.0 g kg -1 APS +1.0 g kg -1 BBR	591:622	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	0	42	theme	immunologic	21:31	arg1	potentiation					33:44	In vitro and in vivo immunologic potentiation	0:44	In vitro and in vivo immunologic potentiation of herb extracts on shrimp (Litopenaeus vannamei).	0:95	In vitro and in vivo immunologic potentiation of herb extracts on shrimp (Litopenaeus vannamei).
33161092	4	43	theme	APS	603:605	arg1	BBR					586:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	43	theme	APS	603:605	arg1	BBR					620:622	1.0 g kg -1 APS +1.0 g kg -1 BBR	591:622	1.0 g kg -1 APS +1.0 g kg -1 BBR	591:622	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	6	44	used	used	1276:1279	arg2	which					1266:1270	which	1266:1270	which	1266:1270	Therefore, the appropriate immunostimulants formula in this study was the combination of 0.5 g kg -1 APS and 0.5 g kg-1 CGA, which was used for 6 days followed by 6 days post-withdrawal.
33161092	4	45	theme	APS + 1.0 g kg	671:684	arg1	BBR					586:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	45	theme	APS + 1.0 g kg	671:684	arg1	CGA					689:691	1.0 g kg -1 APS + 1.0 g kg -1 CGA	659:691	1.0 g kg -1 APS + 1.0 g kg -1 CGA	659:691	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	1	46	theme	chlorogenic	162:172	arg1	CGA					180:182	CGA	180:182	CGA	180:182	In vitro and in vivo effects of Astragalus polysaccharide (APS), chlorogenic acid (CGA) and berberine (BBR) on shrimp (Litopenaeus vannamei) were studied.
33161092	1	46	theme	chlorogenic	162:172	arg1	acid					174:177	chlorogenic acid	162:177	chlorogenic acid (CGA)	162:183	In vitro and in vivo effects of Astragalus polysaccharide (APS), chlorogenic acid (CGA) and berberine (BBR) on shrimp (Litopenaeus vannamei) were studied.
33161092	2	47	theme	enhancement	363:373	arg1	effects					375:381	strong immune enhancement effects	349:381	strong immune enhancement effects	349:381	In vitro test showed that the combination of APS and BBR and the combination of APS and CGA have strong immune enhancement effects and no lysosomal membrane damage on hemocyte.
33161092	5	48	theme	antibacterial	1002:1014	arg1	activities					1016:1025	antibacterial activities	1002:1025	antibacterial activities	1002:1025	The results indicated that G3 (0.5 g kg -1 APS +0.5 g kg -1 CGA) showed higher total hemocyte counts, phagocytic activities, antibacterial activities and bacteriolytic activities during 6 days feeding, and without biomolecule damages after 6 days post-withdrawal.
33161092	7	49	theme	hemocyte	1446:1453	arg1	culture					1455:1461	primary shrimp hemocyte culture	1431:1461	primary shrimp hemocyte culture	1431:1461	Additionally, our study provides new support for screening composite immunostimulants formula by using primary shrimp hemocyte culture.
33161092	7	50	theme	composite	1387:1395	arg1	formula					1414:1420	composite immunostimulants formula	1387:1420	composite immunostimulants formula	1387:1420	Additionally, our study provides new support for screening composite immunostimulants formula by using primary shrimp hemocyte culture.
33161092	3	51	theme	compound	502:509	arg1	extracts					518:525	compound herbal extracts	502:525	compound herbal extracts	502:525	Then, feeding experiment was proceeded to optimize the concentrations of compound herbal extracts.
33161092	1	52	theme	acid	174:177	arg1	effects					118:124	In vitro and in vivo effects	97:124	In vitro and in vivo effects of Astragalus polysaccharide (APS), chlorogenic acid (CGA) and berberine (BBR) on shrimp (Litopenaeus vannamei)	97:236	In vitro and in vivo effects of Astragalus polysaccharide (APS), chlorogenic acid (CGA) and berberine (BBR) on shrimp (Litopenaeus vannamei) were studied.
33161092	2	53	theme	immune	356:361	arg1	effects					375:381	strong immune enhancement effects	349:381	strong immune enhancement effects	349:381	In vitro test showed that the combination of APS and BBR and the combination of APS and CGA have strong immune enhancement effects and no lysosomal membrane damage on hemocyte.
33161092	6	54	from	formula	1185:1191	arg1	study					1201:1205	this study	1196:1205	this study	1196:1205	Therefore, the appropriate immunostimulants formula in this study was the combination of 0.5 g kg -1 APS and 0.5 g kg-1 CGA, which was used for 6 days followed by 6 days post-withdrawal.
33161092	2	55	theme	lysosomal	390:398	arg1	damage					409:414	no lysosomal membrane damage	387:414	no lysosomal membrane damage	387:414	In vitro test showed that the combination of APS and BBR and the combination of APS and CGA have strong immune enhancement effects and no lysosomal membrane damage on hemocyte.
33161092	4	56	theme	+1.0 g kg	607:615	arg1	BBR					586:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	56	theme	+1.0 g kg	607:615	arg1	BBR					620:622	1.0 g kg -1 APS +1.0 g kg -1 BBR	591:622	1.0 g kg -1 APS +1.0 g kg -1 BBR	591:622	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	2	57	theme	strong	349:354	arg1	effects					375:381	strong immune enhancement effects	349:381	strong immune enhancement effects	349:381	In vitro test showed that the combination of APS and BBR and the combination of APS and CGA have strong immune enhancement effects and no lysosomal membrane damage on hemocyte.
33161092	5	58	theme	0.5 g kg	908:915	arg1	CGA					937:939	0.5 g kg -1 APS +0.5 g kg -1 CGA	908:939	0.5 g kg -1 APS +0.5 g kg -1 CGA	908:939	The results indicated that G3 (0.5 g kg -1 APS +0.5 g kg -1 CGA) showed higher total hemocyte counts, phagocytic activities, antibacterial activities and bacteriolytic activities during 6 days feeding, and without biomolecule damages after 6 days post-withdrawal.
33161092	5	58	theme	0.5 g kg	908:915	arg1	G3					904:905	G3	904:905	G3 (0.5 g kg -1 APS +0.5 g kg -1 CGA)	904:940	The results indicated that G3 (0.5 g kg -1 APS +0.5 g kg -1 CGA) showed higher total hemocyte counts, phagocytic activities, antibacterial activities and bacteriolytic activities during 6 days feeding, and without biomolecule damages after 6 days post-withdrawal.
33161092	4	59	theme	lipid	836:840	arg1	peroxidation					842:853	lipid peroxidation	836:853	lipid peroxidation	836:853	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	1	60	theme	In	97:98	arg1	effects					118:124	In vitro and in vivo effects	97:124	In vitro and in vivo effects of Astragalus polysaccharide (APS), chlorogenic acid (CGA) and berberine (BBR) on shrimp (Litopenaeus vannamei)	97:236	In vitro and in vivo effects of Astragalus polysaccharide (APS), chlorogenic acid (CGA) and berberine (BBR) on shrimp (Litopenaeus vannamei) were studied.
33161092	3	61	theme	extracts	518:525	arg1	concentrations					484:497	the concentrations	480:497	the concentrations of compound herbal extracts	480:525	Then, feeding experiment was proceeded to optimize the concentrations of compound herbal extracts.
33161092	6	62	theme	appropriate	1156:1166	arg1	formula					1185:1191	the appropriate immunostimulants formula	1152:1191	the appropriate immunostimulants formula in this study	1152:1205	Therefore, the appropriate immunostimulants formula in this study was the combination of 0.5 g kg -1 APS and 0.5 g kg-1 CGA, which was used for 6 days followed by 6 days post-withdrawal.
33161092	6	62	theme	appropriate	1156:1166	arg1	combination					1215:1225	the combination	1211:1225	the combination of 0.5 g kg -1 APS and 0.5 g kg-1 CGA	1211:1263	Therefore, the appropriate immunostimulants formula in this study was the combination of 0.5 g kg -1 APS and 0.5 g kg-1 CGA, which was used for 6 days followed by 6 days post-withdrawal.
33161092	7	63	theme	new	1361:1363	arg1	support					1365:1371	new support	1361:1371	new support for screening composite immunostimulants formula by using primary shrimp hemocyte culture	1361:1461	Additionally, our study provides new support for screening composite immunostimulants formula by using primary shrimp hemocyte culture.
33161092	0	64	dep	in	13:14	arg1	vivo					16:19	vivo	16:19	vivo	16:19	In vitro and in vivo immunologic potentiation of herb extracts on shrimp (Litopenaeus vannamei).
33161092	4	65	dep	G1-G4	550:554	arg1	BBR					586:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	65	dep	G1-G4	550:554	arg1	CGA					654:656	0.5 g kg -1 APS +0.5 g kg -1 CGA	625:656	0.5 g kg -1 APS +0.5 g kg -1 CGA	625:656	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	65	dep	G1-G4	550:554	arg1	CGA					689:691	1.0 g kg -1 APS + 1.0 g kg -1 CGA	659:691	1.0 g kg -1 APS + 1.0 g kg -1 CGA	659:691	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	65	dep	G1-G4	550:554	arg1	BBR					620:622	1.0 g kg -1 APS +1.0 g kg -1 BBR	591:622	1.0 g kg -1 APS +1.0 g kg -1 BBR	591:622	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	2	66	theme	In	252:253	arg1	test					261:264	In vitro test	252:264	In vitro test	252:264	In vitro test showed that the combination of APS and BBR and the combination of APS and CGA have strong immune enhancement effects and no lysosomal membrane damage on hemocyte.
33161092	4	67	theme	APS	637:639	arg1	BBR					586:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	67	theme	APS	637:639	arg1	CGA					654:656	0.5 g kg -1 APS +0.5 g kg -1 CGA	625:656	0.5 g kg -1 APS +0.5 g kg -1 CGA	625:656	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	5	68	theme	biomolecule	1091:1101	arg1	damages					1103:1109	biomolecule damages	1091:1109	biomolecule damages	1091:1109	The results indicated that G3 (0.5 g kg -1 APS +0.5 g kg -1 CGA) showed higher total hemocyte counts, phagocytic activities, antibacterial activities and bacteriolytic activities during 6 days feeding, and without biomolecule damages after 6 days post-withdrawal.
33161092	6	69	theme	0.5 g kg-1	1250:1259	arg1	CGA					1261:1263	0.5 g kg-1 CGA	1250:1263	0.5 g kg-1 CGA	1250:1263	Therefore, the appropriate immunostimulants formula in this study was the combination of 0.5 g kg -1 APS and 0.5 g kg-1 CGA, which was used for 6 days followed by 6 days post-withdrawal.
33161092	5	70	theme	APS	920:922	arg1	CGA					937:939	0.5 g kg -1 APS +0.5 g kg -1 CGA	908:939	0.5 g kg -1 APS +0.5 g kg -1 CGA	908:939	The results indicated that G3 (0.5 g kg -1 APS +0.5 g kg -1 CGA) showed higher total hemocyte counts, phagocytic activities, antibacterial activities and bacteriolytic activities during 6 days feeding, and without biomolecule damages after 6 days post-withdrawal.
33161092	5	70	theme	APS	920:922	arg1	G3					904:905	G3	904:905	G3 (0.5 g kg -1 APS +0.5 g kg -1 CGA)	904:940	The results indicated that G3 (0.5 g kg -1 APS +0.5 g kg -1 CGA) showed higher total hemocyte counts, phagocytic activities, antibacterial activities and bacteriolytic activities during 6 days feeding, and without biomolecule damages after 6 days post-withdrawal.
33161092	4	71	theme	1.0 g kg	659:666	arg1	BBR					586:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	71	theme	1.0 g kg	659:666	arg1	CGA					689:691	1.0 g kg -1 APS + 1.0 g kg -1 CGA	659:691	1.0 g kg -1 APS + 1.0 g kg -1 CGA	659:691	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	6	72	dep	combination	1215:1225	arg1	used					1276:1279	used	1276:1279	was used for 6 days followed by 6 days post-withdrawal	1272:1325	Therefore, the appropriate immunostimulants formula in this study was the combination of 0.5 g kg -1 APS and 0.5 g kg-1 CGA, which was used for 6 days followed by 6 days post-withdrawal.
33161092	4	73	theme	biomolecule	777:787	arg1	damage					789:794	their biomolecule damage	771:794	their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl	771:874	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	0	74	theme	extracts	54:61	arg1	potentiation					33:44	In vitro and in vivo immunologic potentiation	0:44	In vitro and in vivo immunologic potentiation of herb extracts on shrimp (Litopenaeus vannamei).	0:95	In vitro and in vivo immunologic potentiation of herb extracts on shrimp (Litopenaeus vannamei).
33161092	5	75	theme	+0.5 g kg	924:932	arg1	CGA					937:939	0.5 g kg -1 APS +0.5 g kg -1 CGA	908:939	0.5 g kg -1 APS +0.5 g kg -1 CGA	908:939	The results indicated that G3 (0.5 g kg -1 APS +0.5 g kg -1 CGA) showed higher total hemocyte counts, phagocytic activities, antibacterial activities and bacteriolytic activities during 6 days feeding, and without biomolecule damages after 6 days post-withdrawal.
33161092	5	75	theme	+0.5 g kg	924:932	arg1	G3					904:905	G3	904:905	G3 (0.5 g kg -1 APS +0.5 g kg -1 CGA)	904:940	The results indicated that G3 (0.5 g kg -1 APS +0.5 g kg -1 CGA) showed higher total hemocyte counts, phagocytic activities, antibacterial activities and bacteriolytic activities during 6 days feeding, and without biomolecule damages after 6 days post-withdrawal.
33161092	3	76	theme	herbal	511:516	arg1	extracts					518:525	compound herbal extracts	502:525	compound herbal extracts	502:525	Then, feeding experiment was proceeded to optimize the concentrations of compound herbal extracts.
33161092	1	77	theme	in	110:111	arg1	effects					118:124	In vitro and in vivo effects	97:124	In vitro and in vivo effects of Astragalus polysaccharide (APS), chlorogenic acid (CGA) and berberine (BBR) on shrimp (Litopenaeus vannamei)	97:236	In vitro and in vivo effects of Astragalus polysaccharide (APS), chlorogenic acid (CGA) and berberine (BBR) on shrimp (Litopenaeus vannamei) were studied.
33161092	4	78	theme	0.5 g kg	556:563	arg1	BBR					586:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	0.5 g kg -1 APS + 0.5 g kg -1 BBR	556:588	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	78	theme	0.5 g kg	556:563	arg1	CGA					654:656	0.5 g kg -1 APS +0.5 g kg -1 CGA	625:656	0.5 g kg -1 APS +0.5 g kg -1 CGA	625:656	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	78	theme	0.5 g kg	556:563	arg1	CGA					689:691	1.0 g kg -1 APS + 1.0 g kg -1 CGA	659:691	1.0 g kg -1 APS + 1.0 g kg -1 CGA	659:691	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	4	78	theme	0.5 g kg	556:563	arg1	BBR					620:622	1.0 g kg -1 APS +1.0 g kg -1 BBR	591:622	1.0 g kg -1 APS +1.0 g kg -1 BBR	591:622	Four diets containing G1-G4(0.5 g kg -1 APS + 0.5 g kg -1 BBR, 1.0 g kg -1 APS +1.0 g kg -1 BBR, 0.5 g kg -1 APS +0.5 g kg -1 CGA, 1.0 g kg -1 APS + 1.0 g kg -1 CGA) associated with the control group (common diet) were compared and determined their biomolecule damage to hepatopancreas including DNA damage, lipid peroxidation and protein carbonyl.
33161092	0	79	theme	herb	49:52	arg1	extracts					54:61	herb extracts	49:61	herb extracts	49:61	In vitro and in vivo immunologic potentiation of herb extracts on shrimp (Litopenaeus vannamei).
33161092	0	80	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro and in vivo immunologic potentiation of herb extracts on shrimp (Litopenaeus vannamei).
33161092	6	81	theme	APS	1242:1244	arg1	formula					1185:1191	the appropriate immunostimulants formula	1152:1191	the appropriate immunostimulants formula in this study	1152:1205	Therefore, the appropriate immunostimulants formula in this study was the combination of 0.5 g kg -1 APS and 0.5 g kg-1 CGA, which was used for 6 days followed by 6 days post-withdrawal.
33161092	6	81	theme	APS	1242:1244	arg1	combination					1215:1225	the combination	1211:1225	the combination of 0.5 g kg -1 APS and 0.5 g kg-1 CGA	1211:1263	Therefore, the appropriate immunostimulants formula in this study was the combination of 0.5 g kg -1 APS and 0.5 g kg-1 CGA, which was used for 6 days followed by 6 days post-withdrawal.
33593532	3	0	from	analysis	501:508	arg1	composite					537:545	the composite	533:545	the composite	533:545	Quantitative analysis of chitosan content in the composite was conducted by tritium-labeled chitosan radioactivity detection method and this showed three times increased chitosan loading.
33593532	2	1	theme	such	335:338	arg1	composites					340:349	such composites	335:349	such composites	335:349	The creation of such composites in the carbonic acid solutions that were performed in this work has several advantages in terms of being biocompatible and eco-friendly.
33593532	3	2	from	content	522:528	arg1	composite					537:545	the composite	533:545	the composite	533:545	Quantitative analysis of chitosan content in the composite was conducted by tritium-labeled chitosan radioactivity detection method and this showed three times increased chitosan loading.
33593532	6	3	theme	surface	1000:1006	arg1	area					1008:1011	the inner specific surface area	981:1011	the inner specific surface area	981:1011	It was found that the inner specific surface area increased 1.5 times on deposition of chitosan from the solutions in carbonic acid.
33593532	1	4	theme	bacterial	162:170	arg1	surface					187:193	the bacterial cellulose (BC) surface	158:193	the bacterial cellulose (BC) surface	158:193	The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC is an important scientific task.
33593532	6	5	theme	specific	991:998	arg1	area					1008:1011	the inner specific surface area	981:1011	the inner specific surface area	981:1011	It was found that the inner specific surface area increased 1.5 times on deposition of chitosan from the solutions in carbonic acid.
33593532	0	6	theme	acid	103:106	arg1	solutions					81:89	the solutions	77:89	the solutions of carbonic acid under high pressure CO2	77:130	Green approach for fabrication of bacterial cellulose-chitosan composites in the solutions of carbonic acid under high pressure CO2.
33593532	1	7	with	functionalization	137:153	arg1	biopolymer					211:220	a chitosan biopolymer	200:220	a chitosan biopolymer to expand the areas of possible applications of the modified BC	200:284	The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC is an important scientific task.
33593532	2	8	from	creation	323:330	arg1	solutions					372:380	the carbonic acid solutions	354:380	the carbonic acid solutions that were performed in this work	354:413	The creation of such composites in the carbonic acid solutions that were performed in this work has several advantages in terms of being biocompatible and eco-friendly.
33593532	1	9	theme	possible	245:252	arg1	applications					254:265	possible applications	245:265	possible applications of the modified BC	245:284	The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC is an important scientific task.
33593532	0	10	theme	carbonic	94:101	arg1	acid					103:106	carbonic acid	94:106	carbonic acid under high pressure CO2	94:130	Green approach for fabrication of bacterial cellulose-chitosan composites in the solutions of carbonic acid under high pressure CO2.
33593532	0	11	from	composites	63:72	arg1	solutions					81:89	the solutions	77:89	the solutions of carbonic acid under high pressure CO2	77:130	Green approach for fabrication of bacterial cellulose-chitosan composites in the solutions of carbonic acid under high pressure CO2.
33593532	2	12	contain	has	415:417	arg1	creation					323:330	The creation	319:330	The creation of such composites in the carbonic acid solutions that were performed in this work	319:413	The creation of such composites in the carbonic acid solutions that were performed in this work has several advantages in terms of being biocompatible and eco-friendly.
33593532	2	12	contain	has	415:417	arg2	advantages					427:436	several advantages	419:436	several advantages	419:436	The creation of such composites in the carbonic acid solutions that were performed in this work has several advantages in terms of being biocompatible and eco-friendly.
33593532	1	13	theme	applications	254:265	arg1	areas					236:240	the areas	232:240	the areas of possible applications of the modified BC	232:284	The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC is an important scientific task.
33593532	0	14	theme	high	114:117	arg1	pressure					119:126	high pressure CO2	114:130	high pressure CO2	114:130	Green approach for fabrication of bacterial cellulose-chitosan composites in the solutions of carbonic acid under high pressure CO2.
33593532	6	15	from	solutions	1068:1076	arg1	acid					1090:1093	carbonic acid	1081:1093	carbonic acid	1081:1093	It was found that the inner specific surface area increased 1.5 times on deposition of chitosan from the solutions in carbonic acid.
33593532	3	16	theme	chitosan	513:520	arg1	content					522:528	chitosan content	513:528	chitosan content in the composite	513:545	Quantitative analysis of chitosan content in the composite was conducted by tritium-labeled chitosan radioactivity detection method and this showed three times increased chitosan loading.
33593532	2	17	theme	carbonic	358:365	arg1	solutions					372:380	the carbonic acid solutions	354:380	the carbonic acid solutions that were performed in this work	354:413	The creation of such composites in the carbonic acid solutions that were performed in this work has several advantages in terms of being biocompatible and eco-friendly.
33593532	1	18	theme	cellulose	172:180	arg1	surface					187:193	the bacterial cellulose (BC) surface	158:193	the bacterial cellulose (BC) surface	158:193	The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC is an important scientific task.
33593532	3	19	theme	content	522:528	arg1	analysis					501:508	Quantitative analysis	488:508	Quantitative analysis of chitosan content in the composite	488:545	Quantitative analysis of chitosan content in the composite was conducted by tritium-labeled chitosan radioactivity detection method and this showed three times increased chitosan loading.
33593532	6	20	theme	carbonic	1081:1088	arg1	acid					1090:1093	carbonic acid	1081:1093	carbonic acid	1081:1093	It was found that the inner specific surface area increased 1.5 times on deposition of chitosan from the solutions in carbonic acid.
33593532	2	21	theme	acid	367:370	arg1	solutions					372:380	the carbonic acid solutions	354:380	the carbonic acid solutions that were performed in this work	354:413	The creation of such composites in the carbonic acid solutions that were performed in this work has several advantages in terms of being biocompatible and eco-friendly.
33593532	2	22	theme	several	419:425	arg1	advantages					427:436	several advantages	419:436	several advantages	419:436	The creation of such composites in the carbonic acid solutions that were performed in this work has several advantages in terms of being biocompatible and eco-friendly.
33593532	1	23	theme	BC	183:184	arg1	surface					187:193	the bacterial cellulose (BC) surface	158:193	the bacterial cellulose (BC) surface	158:193	The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC is an important scientific task.
33593532	0	24	theme	Green	0:4	arg1	approach					6:13	Green approach	0:13	Green approach for fabrication of bacterial cellulose-chitosan composites in the solutions of carbonic acid under high pressure CO2.	0:131	Green approach for fabrication of bacterial cellulose-chitosan composites in the solutions of carbonic acid under high pressure CO2.
33593532	4	25	theme	BC	763:764	arg1	matrix					766:771	the BC matrix	759:771	the BC matrix	759:771	Different physicochemical methods showed successful incorporation of chitosan into the BC matrix and interaction with it through hydrogen bonds.
33593532	3	26	theme	chitosan	658:665	arg1	loading					667:673	chitosan loading	658:673	chitosan loading	658:673	Quantitative analysis of chitosan content in the composite was conducted by tritium-labeled chitosan radioactivity detection method and this showed three times increased chitosan loading.
33593532	1	27	theme	surface	187:193	arg1	task					313:316	an important scientific task	289:316	an important scientific task	289:316	The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC is an important scientific task.
33593532	1	27	theme	surface	187:193	arg1	functionalization					137:153	The functionalization	133:153	The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC	133:284	The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC is an important scientific task.
33593532	1	28	theme	modified	274:281	arg1	BC					283:284	the modified BC	270:284	the modified BC	270:284	The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC is an important scientific task.
33593532	4	29	theme	hydrogen	805:812	arg1	bonds					814:818	hydrogen bonds	805:818	hydrogen bonds	805:818	Different physicochemical methods showed successful incorporation of chitosan into the BC matrix and interaction with it through hydrogen bonds.
33593532	3	30	theme	Quantitative	488:499	arg1	analysis					501:508	Quantitative analysis	488:508	Quantitative analysis of chitosan content in the composite	488:545	Quantitative analysis of chitosan content in the composite was conducted by tritium-labeled chitosan radioactivity detection method and this showed three times increased chitosan loading.
33593532	0	31	theme	bacterial	34:42	arg1	composites					63:72	bacterial cellulose-chitosan composites	34:72	bacterial cellulose-chitosan composites in the solutions of carbonic acid under high pressure CO2	34:130	Green approach for fabrication of bacterial cellulose-chitosan composites in the solutions of carbonic acid under high pressure CO2.
33593532	1	32	theme	BC	283:284	arg1	applications					254:265	possible applications	245:265	possible applications of the modified BC	245:284	The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC is an important scientific task.
33593532	0	33	from	solutions	81:89	arg1	fabrication					19:29	fabrication	19:29	fabrication of bacterial cellulose-chitosan composites in the solutions of carbonic acid under high pressure CO2	19:130	Green approach for fabrication of bacterial cellulose-chitosan composites in the solutions of carbonic acid under high pressure CO2.
33593532	3	34	theme	radioactivity	589:601	arg1	method					613:618	tritium-labeled chitosan radioactivity detection method	564:618	tritium-labeled chitosan radioactivity detection method	564:618	Quantitative analysis of chitosan content in the composite was conducted by tritium-labeled chitosan radioactivity detection method and this showed three times increased chitosan loading.
33593532	4	35	theme	Different	676:684	arg1	methods					702:708	Different physicochemical methods	676:708	Different physicochemical methods	676:708	Different physicochemical methods showed successful incorporation of chitosan into the BC matrix and interaction with it through hydrogen bonds.
33593532	4	36	with	interaction	777:787	arg1	it					794:795	it	794:795	it	794:795	Different physicochemical methods showed successful incorporation of chitosan into the BC matrix and interaction with it through hydrogen bonds.
33593532	3	37	theme	detection	603:611	arg1	method					613:618	tritium-labeled chitosan radioactivity detection method	564:618	tritium-labeled chitosan radioactivity detection method	564:618	Quantitative analysis of chitosan content in the composite was conducted by tritium-labeled chitosan radioactivity detection method and this showed three times increased chitosan loading.
33593532	1	38	theme	chitosan	202:209	arg1	biopolymer					211:220	a chitosan biopolymer	200:220	a chitosan biopolymer to expand the areas of possible applications of the modified BC	200:284	The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC is an important scientific task.
33593532	0	39	theme	composites	63:72	arg1	fabrication					19:29	fabrication	19:29	fabrication of bacterial cellulose-chitosan composites in the solutions of carbonic acid under high pressure CO2	19:130	Green approach for fabrication of bacterial cellulose-chitosan composites in the solutions of carbonic acid under high pressure CO2.
33593532	4	40	theme	physicochemical	686:700	arg1	methods					702:708	Different physicochemical methods	676:708	Different physicochemical methods	676:708	Different physicochemical methods showed successful incorporation of chitosan into the BC matrix and interaction with it through hydrogen bonds.
33593532	3	41	theme	tritium-labeled	564:578	arg1	method					613:618	tritium-labeled chitosan radioactivity detection method	564:618	tritium-labeled chitosan radioactivity detection method	564:618	Quantitative analysis of chitosan content in the composite was conducted by tritium-labeled chitosan radioactivity detection method and this showed three times increased chitosan loading.
33593532	4	42	theme	chitosan	745:752	arg1	incorporation					728:740	successful incorporation	717:740	successful incorporation of chitosan into the BC matrix	717:771	Different physicochemical methods showed successful incorporation of chitosan into the BC matrix and interaction with it through hydrogen bonds.
33593532	4	42	theme	chitosan	745:752	arg1	interaction					777:787	interaction	777:787	interaction with it through hydrogen bonds	777:818	Different physicochemical methods showed successful incorporation of chitosan into the BC matrix and interaction with it through hydrogen bonds.
33593532	0	43	theme	cellulose-chitosan	44:61	arg1	composites					63:72	bacterial cellulose-chitosan composites	34:72	bacterial cellulose-chitosan composites in the solutions of carbonic acid under high pressure CO2	34:130	Green approach for fabrication of bacterial cellulose-chitosan composites in the solutions of carbonic acid under high pressure CO2.
33593532	1	44	theme	important	292:300	arg1	task					313:316	an important scientific task	289:316	an important scientific task	289:316	The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC is an important scientific task.
33593532	1	44	theme	important	292:300	arg1	functionalization					137:153	The functionalization	133:153	The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC	133:284	The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC is an important scientific task.
33593532	3	45	theme	chitosan	580:587	arg1	method					613:618	tritium-labeled chitosan radioactivity detection method	564:618	tritium-labeled chitosan radioactivity detection method	564:618	Quantitative analysis of chitosan content in the composite was conducted by tritium-labeled chitosan radioactivity detection method and this showed three times increased chitosan loading.
33593532	3	46	from	composite	537:545	arg1	analysis					501:508	Quantitative analysis	488:508	Quantitative analysis of chitosan content in the composite	488:545	Quantitative analysis of chitosan content in the composite was conducted by tritium-labeled chitosan radioactivity detection method and this showed three times increased chitosan loading.
33593532	1	47	theme	scientific	302:311	arg1	task					313:316	an important scientific task	289:316	an important scientific task	289:316	The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC is an important scientific task.
33593532	1	47	theme	scientific	302:311	arg1	functionalization					137:153	The functionalization	133:153	The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC	133:284	The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC is an important scientific task.
33593532	4	48	with	incorporation	728:740	arg1	it					794:795	it	794:795	it	794:795	Different physicochemical methods showed successful incorporation of chitosan into the BC matrix and interaction with it through hydrogen bonds.
33593532	5	49	theme	BC	932:933	arg1	bulk					924:927	the bulk	920:927	the bulk of BC	920:933	Microscopy results showed that the chitosan coating with a thickness of around 10 nm was formed in the bulk of BC, covering each microfibril.
33593532	4	50	theme	successful	717:726	arg1	incorporation					728:740	successful incorporation	717:740	successful incorporation of chitosan into the BC matrix	717:771	Different physicochemical methods showed successful incorporation of chitosan into the BC matrix and interaction with it through hydrogen bonds.
33593532	6	51	theme	inner	985:989	arg1	area					1008:1011	the inner specific surface area	981:1011	the inner specific surface area	981:1011	It was found that the inner specific surface area increased 1.5 times on deposition of chitosan from the solutions in carbonic acid.
33593532	3	52	dep	showed	629:634	arg1	increased					648:656	increased	648:656	showed three times increased chitosan loading	629:673	Quantitative analysis of chitosan content in the composite was conducted by tritium-labeled chitosan radioactivity detection method and this showed three times increased chitosan loading.
33593532	6	53	theme	chitosan	1050:1057	arg1	deposition					1036:1045	deposition	1036:1045	deposition of chitosan	1036:1057	It was found that the inner specific surface area increased 1.5 times on deposition of chitosan from the solutions in carbonic acid.
33593532	5	54	theme	nm	903:904	arg1	thickness					880:888	a thickness	878:888	a thickness of around 10 nm	878:904	Microscopy results showed that the chitosan coating with a thickness of around 10 nm was formed in the bulk of BC, covering each microfibril.
33593532	5	55	theme	Microscopy	821:830	arg1	results					832:838	Microscopy results	821:838	Microscopy results	821:838	Microscopy results showed that the chitosan coating with a thickness of around 10 nm was formed in the bulk of BC, covering each microfibril.
33593532	0	56	from	fabrication	19:29	arg1	solutions					81:89	the solutions	77:89	the solutions of carbonic acid under high pressure CO2	77:130	Green approach for fabrication of bacterial cellulose-chitosan composites in the solutions of carbonic acid under high pressure CO2.
33593532	2	57	theme	composites	340:349	arg1	creation					323:330	The creation	319:330	The creation of such composites in the carbonic acid solutions that were performed in this work	319:413	The creation of such composites in the carbonic acid solutions that were performed in this work has several advantages in terms of being biocompatible and eco-friendly.
33291979	7	0	theme	min	1231:1233	arg1	span					1221:1224	a short span	1213:1224	a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF))	1213:1400	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	6	1	theme	RF	914:915	arg1	content					917:923	high RF content	909:923	high RF content (80%)	909:929	However, reduction in WF increased the cooking loss, compromised textural and organoleptic attributes in samples with high RF content (80%); nevertheless, addition of GG and MGGF reduced these losses and improved the sensory qualities (p < 0.05).
33291979	6	1	theme	RF	914:915	arg1	%					928:928	80%	926:928	80%	926:928	However, reduction in WF increased the cooking loss, compromised textural and organoleptic attributes in samples with high RF content (80%); nevertheless, addition of GG and MGGF reduced these losses and improved the sensory qualities (p < 0.05).
33291979	2	2	theme	malted	295:300	arg1	flour					313:317	malted green gram flour	295:317	malted green gram flour (MGGF, 5 and 10%)	295:335	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	9	3	dep	digestibility	1835:1847	arg1	the					1798:1800	the	1798:1800	the	1798:1800	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	8	4	theme	cooked	1541:1546	arg1	counterparts					1548:1559	their cooked counterparts	1535:1559	their cooked counterparts	1535:1559	Adhesiveness of the rehydrated samples was lesser than their cooked counterparts, while vice versa was observed in case of their instrumental firmness/hardness.
33291979	7	5	theme	short	1215:1219	arg1	span					1221:1224	a short span	1213:1224	a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF))	1213:1400	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	9	6	theme	high	1739:1742	arg1	content					1752:1758	high protein content	1739:1758	high protein content (∼1.17-1.29 times higher than T0)	1739:1792	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	1	7	with	pasta	122:126	arg1	qualities					178:186	improved cooking/rehydrating and nutritional qualities	133:186	improved cooking/rehydrating and nutritional qualities	133:186	The present study was undertaken to develop rice-based composite pasta with improved cooking/rehydrating and nutritional qualities.
33291979	10	8	theme	low-cost	1904:1911	arg1	pasta					1939:1943	low-cost quick-cooking/rehydrating pasta	1904:1943	low-cost quick-cooking/rehydrating pasta	1904:1943	Thus low-cost quick-cooking/rehydrating pasta can be successfully developed by adding suitable amount of MGGF and GG into rice-wheat composite flours.
33291979	7	9	from	panel	1473:1477	arg1	preference					1445:1454	higher preference	1438:1454	higher preference from the sensory panel	1438:1477	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	2	10	dep	flour	275:279	arg1	WF					282:283	WF	282:283	WF	282:283	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	2	10	dep	flour	275:279	arg1	%					291:291	40-12%	286:291	40-12%	286:291	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	2	11	dep	flour	249:253	arg1	RF					256:257	RF	256:257	RF	256:257	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	2	11	dep	flour	249:253	arg1	%					265:265	60-80%	260:265	60-80%	260:265	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	9	12	theme	higher	1778:1783	arg1	times					1772:1776	∼1.17-1.29 times	1761:1776	∼1.17-1.29 times higher than T0	1761:1791	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	2	13	theme	rice	244:247	arg1	flour					249:253	rice flour	244:253	rice flour (RF, 60-80%)	244:266	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	7	14	theme	acceptable	1352:1361	arg1	≥5					1370:1371	≥5	1370:1371	≥5	1370:1371	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	7	14	theme	acceptable	1352:1361	arg1	range					1363:1367	acceptable range	1352:1367	acceptable range (≥5) (except T3 (80%RF + 20%WF))	1352:1400	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	6	15	theme	sensory	1008:1014	arg1	p < 0.05					1027:1034	p < 0.05	1027:1034	p < 0.05	1027:1034	However, reduction in WF increased the cooking loss, compromised textural and organoleptic attributes in samples with high RF content (80%); nevertheless, addition of GG and MGGF reduced these losses and improved the sensory qualities (p < 0.05).
33291979	6	15	theme	sensory	1008:1014	arg1	qualities					1016:1024	the sensory qualities	1004:1024	the sensory qualities (p < 0.05)	1004:1035	However, reduction in WF increased the cooking loss, compromised textural and organoleptic attributes in samples with high RF content (80%); nevertheless, addition of GG and MGGF reduced these losses and improved the sensory qualities (p < 0.05).
33291979	7	16	theme	cooked	1419:1424	arg1	ones					1426:1429	nonetheless the cooked ones	1403:1429	nonetheless the cooked ones	1403:1429	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	4	17	theme	cooking	556:562	arg1	loss					564:567	cooking loss	556:567	cooking loss	556:567	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	4	17	theme	cooking	556:562	arg1	properties					522:531	their cooking properties	508:531	their cooking properties (optimum cooking time, cooking loss and swelling index)	508:587	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	8	18	theme	instrumental	1609:1620	arg1	firmness/hardness					1622:1638	their instrumental firmness/hardness	1603:1638	their instrumental firmness/hardness	1603:1638	Adhesiveness of the rehydrated samples was lesser than their cooked counterparts, while vice versa was observed in case of their instrumental firmness/hardness.
33291979	1	19	theme	improved	133:140	arg1	qualities					178:186	improved cooking/rehydrating and nutritional qualities	133:186	improved cooking/rehydrating and nutritional qualities	133:186	The present study was undertaken to develop rice-based composite pasta with improved cooking/rehydrating and nutritional qualities.
33291979	9	20	theme	%	1660:1660	arg1	%					1685:1685	70%RF + 19%WF + 10%MGGF + 1%GG	1658:1687	70%RF + 19%WF + 10%MGGF + 1%GG	1658:1687	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	9	20	theme	%	1660:1660	arg1	T7					1654:1655	T7	1654:1655	T7 (70%RF + 19%WF + 10%MGGF + 1%GG)	1654:1688	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	4	21	theme	optimum	534:540	arg1	time					550:553	optimum cooking time	534:553	optimum cooking time	534:553	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	4	21	theme	optimum	534:540	arg1	properties					522:531	their cooking properties	508:531	their cooking properties (optimum cooking time, cooking loss and swelling index)	508:587	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	9	22	theme	low	1815:1817	arg1	digestibility					1835:1847	low in vitro starch digestibility	1815:1847	low in vitro starch digestibility	1815:1847	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	9	22	theme	low	1815:1817	arg1	reduction					1887:1895	∼1.16-1.25 fold reduction	1871:1895	∼1.16-1.25 fold reduction	1871:1895	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	10	23	theme	MGGF	2004:2007	arg1	amount					1994:1999	suitable amount	1985:1999	suitable amount of MGGF and GG	1985:2014	Thus low-cost quick-cooking/rehydrating pasta can be successfully developed by adding suitable amount of MGGF and GG into rice-wheat composite flours.
33291979	10	23	theme	MGGF	2004:2007	arg1	GG					2013:2014	GG	2013:2014	GG	2013:2014	Thus low-cost quick-cooking/rehydrating pasta can be successfully developed by adding suitable amount of MGGF and GG into rice-wheat composite flours.
33291979	10	23	theme	MGGF	2004:2007	arg1	MGGF					2004:2007	MGGF	2004:2007	MGGF	2004:2007	Thus low-cost quick-cooking/rehydrating pasta can be successfully developed by adding suitable amount of MGGF and GG into rice-wheat composite flours.
33291979	9	24	theme	%	1668:1668	arg1	%					1685:1685	70%RF + 19%WF + 10%MGGF + 1%GG	1658:1687	70%RF + 19%WF + 10%MGGF + 1%GG	1658:1687	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	9	24	theme	%	1668:1668	arg1	T7					1654:1655	T7	1654:1655	T7 (70%RF + 19%WF + 10%MGGF + 1%GG)	1654:1688	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	6	25	theme	MGGF	965:968	arg1	addition					946:953	addition	946:953	addition of GG and MGGF	946:968	However, reduction in WF increased the cooking loss, compromised textural and organoleptic attributes in samples with high RF content (80%); nevertheless, addition of GG and MGGF reduced these losses and improved the sensory qualities (p < 0.05).
33291979	1	26	theme	present	61:67	arg1	study					69:73	The present study	57:73	The present study	57:73	The present study was undertaken to develop rice-based composite pasta with improved cooking/rehydrating and nutritional qualities.
33291979	7	27	theme	RF + 20	1389:1395	arg1	T3					1382:1383	T3	1382:1383	T3 (80%RF + 20%WF)	1382:1399	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	7	27	theme	RF + 20	1389:1395	arg1	WF					1397:1398	80%RF + 20%WF	1386:1398	80%RF + 20%WF	1386:1398	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	2	28	theme	single	383:388	arg1	extruder					396:403	a single screw extruder	381:403	a single screw extruder	381:403	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	9	29	theme	%	1676:1676	arg1	%					1685:1685	70%RF + 19%WF + 10%MGGF + 1%GG	1658:1687	70%RF + 19%WF + 10%MGGF + 1%GG	1658:1687	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	9	29	theme	%	1676:1676	arg1	T7					1654:1655	T7	1654:1655	T7 (70%RF + 19%WF + 10%MGGF + 1%GG)	1654:1688	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	2	30	dep	flour	313:317	arg1	%					334:334	5 and 10%	326:334	5 and 10%	326:334	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	2	30	dep	flour	313:317	arg1	MGGF					320:323	MGGF	320:323	MGGF	320:323	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	8	31	theme	samples	1511:1517	arg1	Adhesiveness					1480:1491	Adhesiveness	1480:1491	Adhesiveness of the rehydrated samples	1480:1517	Adhesiveness of the rehydrated samples was lesser than their cooked counterparts, while vice versa was observed in case of their instrumental firmness/hardness.
33291979	10	32	theme	rice-wheat	2021:2030	arg1	flours					2042:2047	rice-wheat composite flours	2021:2047	rice-wheat composite flours	2021:2047	Thus low-cost quick-cooking/rehydrating pasta can be successfully developed by adding suitable amount of MGGF and GG into rice-wheat composite flours.
33291979	9	33	theme	calorific	1853:1861	arg1	values					1863:1868	calorific values	1853:1868	calorific values	1853:1868	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	2	34	dep	gum	346:348	arg1	GG					351:352	GG	351:352	GG	351:352	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	2	34	dep	gum	346:348	arg1	%					358:358	1-3%	355:358	1-3%	355:358	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	7	35	theme	cooking	1103:1109	arg1	time					1111:1114	the optimal cooking time	1091:1114	the optimal cooking time	1091:1114	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	2	36	theme	guar	341:344	arg1	gum					346:348	guar gum	341:348	guar gum (GG, 1-3%)	341:359	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	0	37	theme	composite	19:27	arg1	pasta					29:33	composite pasta	19:33	composite pasta requiring no cooking	19:54	Instant rice-based composite pasta requiring no cooking.
33291979	1	38	theme	rice-based	101:110	arg1	pasta					122:126	rice-based composite pasta	101:126	rice-based composite pasta with improved cooking/rehydrating and nutritional qualities	101:186	The present study was undertaken to develop rice-based composite pasta with improved cooking/rehydrating and nutritional qualities.
33291979	5	39	theme	cooking	713:719	arg1	time					721:724	the cooking time	709:724	the cooking time	709:724	Substitution of WF by RF significantly reduced the cooking time, and improved the whiteness and swelling indices of the samples.
33291979	6	40	theme	textural	856:863	arg1	attributes					882:891	textural and organoleptic attributes	856:891	textural and organoleptic attributes in samples with high RF content (80%)	856:929	However, reduction in WF increased the cooking loss, compromised textural and organoleptic attributes in samples with high RF content (80%); nevertheless, addition of GG and MGGF reduced these losses and improved the sensory qualities (p < 0.05).
33291979	4	41	theme	organoleptic	637:648	arg1	parameters					650:659	textural and organoleptic parameters	624:659	textural and organoleptic parameters	624:659	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	4	41	theme	organoleptic	637:648	arg1	properties					522:531	their cooking properties	508:531	their cooking properties (optimum cooking time, cooking loss and swelling index)	508:587	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	9	42	theme	fold	1882:1885	arg1	digestibility					1835:1847	low in vitro starch digestibility	1815:1847	low in vitro starch digestibility	1815:1847	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	9	42	theme	fold	1882:1885	arg1	reduction					1887:1895	∼1.16-1.25 fold reduction	1871:1895	∼1.16-1.25 fold reduction	1871:1895	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	6	43	theme	cooking	830:836	arg1	loss					838:841	the cooking loss	826:841	the cooking loss	826:841	However, reduction in WF increased the cooking loss, compromised textural and organoleptic attributes in samples with high RF content (80%); nevertheless, addition of GG and MGGF reduced these losses and improved the sensory qualities (p < 0.05).
33291979	7	44	theme	pasta	1084:1088	arg1	composition					1058:1068	the composition	1054:1068	the composition of rice-based pasta	1054:1088	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	7	45	theme	textural	1245:1252	arg1	qualities					1254:1262	textural qualities	1245:1262	textural qualities	1245:1262	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	7	46	dep	ranged	1116:1121	arg1	led					1431:1433	led	1431:1433	led to higher preference from the sensory panel	1431:1477	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	9	47	theme	RF + 13	1701:1707	arg1	%					1724:1724	80%RF + 13%WF + 5%MGGF + 2%GG	1698:1726	80%RF + 13%WF + 5%MGGF + 2%GG	1698:1726	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	9	47	theme	RF + 13	1701:1707	arg1	T9					1694:1695	T9	1694:1695	T9 (80%RF + 13%WF + 5%MGGF + 2%GG)	1694:1727	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	9	48	theme	WF + 5	1709:1714	arg1	%					1724:1724	80%RF + 13%WF + 5%MGGF + 2%GG	1698:1726	80%RF + 13%WF + 5%MGGF + 2%GG	1698:1726	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	9	48	theme	WF + 5	1709:1714	arg1	T9					1694:1695	T9	1694:1695	T9 (80%RF + 13%WF + 5%MGGF + 2%GG)	1694:1727	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	4	49	theme	textural	624:631	arg1	parameters					650:659	textural and organoleptic parameters	624:659	textural and organoleptic parameters	624:659	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	4	49	theme	textural	624:631	arg1	properties					522:531	their cooking properties	508:531	their cooking properties (optimum cooking time, cooking loss and swelling index)	508:587	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	2	50	theme	flour	313:317	arg1	proportions					229:239	different proportions	219:239	different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%)	219:359	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	2	51	theme	green	302:306	arg1	flour					313:317	malted green gram flour	295:317	malted green gram flour (MGGF, 5 and 10%)	295:335	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	9	52	dep	content	1752:1758	arg1	times					1772:1776	∼1.17-1.29 times	1761:1776	∼1.17-1.29 times higher than T0	1761:1791	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	7	53	theme	sensory	1465:1471	arg1	panel					1473:1477	the sensory panel	1461:1477	the sensory panel	1461:1477	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	6	54	theme	high	909:912	arg1	content					917:923	high RF content	909:923	high RF content (80%)	909:929	However, reduction in WF increased the cooking loss, compromised textural and organoleptic attributes in samples with high RF content (80%); nevertheless, addition of GG and MGGF reduced these losses and improved the sensory qualities (p < 0.05).
33291979	6	54	theme	high	909:912	arg1	%					928:928	80%	926:928	80%	926:928	However, reduction in WF increased the cooking loss, compromised textural and organoleptic attributes in samples with high RF content (80%); nevertheless, addition of GG and MGGF reduced these losses and improved the sensory qualities (p < 0.05).
33291979	6	55	from	attributes	882:891	arg1	samples					896:902	samples	896:902	samples with high RF content (80%)	896:929	However, reduction in WF increased the cooking loss, compromised textural and organoleptic attributes in samples with high RF content (80%); nevertheless, addition of GG and MGGF reduced these losses and improved the sensory qualities (p < 0.05).
33291979	2	56	theme	wheat	269:273	arg1	flour					275:279	wheat flour	269:279	wheat flour (WF, 40-12%)	269:292	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	7	57	theme	hot	1196:1198	arg1	water					1200:1204	hot water	1196:1204	hot water	1196:1204	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	7	58	theme	higher	1438:1443	arg1	preference					1445:1454	higher preference	1438:1454	higher preference from the sensory panel	1438:1477	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	6	59	theme	GG	958:959	arg1	addition					946:953	addition	946:953	addition of GG and MGGF	946:968	However, reduction in WF increased the cooking loss, compromised textural and organoleptic attributes in samples with high RF content (80%); nevertheless, addition of GG and MGGF reduced these losses and improved the sensory qualities (p < 0.05).
33291979	9	60	theme	protein	1744:1750	arg1	content					1752:1758	high protein content	1739:1758	high protein content (∼1.17-1.29 times higher than T0)	1739:1792	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	9	61	theme	%	1700:1700	arg1	%					1724:1724	80%RF + 13%WF + 5%MGGF + 2%GG	1698:1726	80%RF + 13%WF + 5%MGGF + 2%GG	1698:1726	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	9	61	theme	%	1700:1700	arg1	T9					1694:1695	T9	1694:1695	T9 (80%RF + 13%WF + 5%MGGF + 2%GG)	1694:1727	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	3	62	theme	100	420:422	arg1	%					423:423	%	423:423	%	423:423	Pasta made of 100% WF served as the control (T0).
33291979	7	63	with	par	1267:1269	arg1	scores					1321:1326	sensorial scores	1311:1326	sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF))	1311:1400	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	7	63	with	par	1267:1269	arg1	counterparts					1289:1300	their cooked counterparts	1276:1300	their cooked counterparts	1276:1300	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	7	64	theme	range	1363:1367	arg1	limit					1343:1347	the limit	1339:1347	the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF))	1339:1400	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	4	65	theme	cooked	460:465	arg1	samples					481:487	The cooked or rehydrated samples	456:487	The cooked or rehydrated samples	456:487	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	2	66	theme	flour	249:253	arg1	proportions					229:239	different proportions	219:239	different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%)	219:359	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	5	67	theme	WF	678:679	arg1	Substitution					662:673	Substitution	662:673	Substitution of WF by RF	662:685	Substitution of WF by RF significantly reduced the cooking time, and improved the whiteness and swelling indices of the samples.
33291979	4	68	theme	rehydrated	470:479	arg1	samples					481:487	The cooked or rehydrated samples	456:487	The cooked or rehydrated samples	456:487	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	1	69	theme	composite	112:120	arg1	pasta					122:126	rice-based composite pasta	101:126	rice-based composite pasta with improved cooking/rehydrating and nutritional qualities	101:186	The present study was undertaken to develop rice-based composite pasta with improved cooking/rehydrating and nutritional qualities.
33291979	10	70	theme	GG	2013:2014	arg1	amount					1994:1999	suitable amount	1985:1999	suitable amount of MGGF and GG	1985:2014	Thus low-cost quick-cooking/rehydrating pasta can be successfully developed by adding suitable amount of MGGF and GG into rice-wheat composite flours.
33291979	10	70	theme	GG	2013:2014	arg1	GG					2013:2014	GG	2013:2014	GG	2013:2014	Thus low-cost quick-cooking/rehydrating pasta can be successfully developed by adding suitable amount of MGGF and GG into rice-wheat composite flours.
33291979	10	70	theme	GG	2013:2014	arg1	MGGF					2004:2007	MGGF	2004:2007	MGGF	2004:2007	Thus low-cost quick-cooking/rehydrating pasta can be successfully developed by adding suitable amount of MGGF and GG into rice-wheat composite flours.
33291979	4	71	theme	cooking	542:548	arg1	time					550:553	optimum cooking time	534:553	optimum cooking time	534:553	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	4	71	theme	cooking	542:548	arg1	properties					522:531	their cooking properties	508:531	their cooking properties (optimum cooking time, cooking loss and swelling index)	508:587	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	5	72	theme	whiteness	744:752	arg1	indices					767:773	the whiteness and swelling indices	740:773	the whiteness and swelling indices of the samples	740:788	Substitution of WF by RF significantly reduced the cooking time, and improved the whiteness and swelling indices of the samples.
33291979	10	73	theme	quick-cooking/rehydrating	1913:1937	arg1	pasta					1939:1943	low-cost quick-cooking/rehydrating pasta	1904:1943	low-cost quick-cooking/rehydrating pasta	1904:1943	Thus low-cost quick-cooking/rehydrating pasta can be successfully developed by adding suitable amount of MGGF and GG into rice-wheat composite flours.
33291979	1	74	theme	cooking/rehydrating	142:160	arg1	qualities					178:186	improved cooking/rehydrating and nutritional qualities	133:186	improved cooking/rehydrating and nutritional qualities	133:186	The present study was undertaken to develop rice-based composite pasta with improved cooking/rehydrating and nutritional qualities.
33291979	7	75	theme	sensorial	1311:1319	arg1	scores					1321:1326	sensorial scores	1311:1326	sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF))	1311:1400	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	4	76	theme	cooking	514:520	arg1	time					550:553	optimum cooking time	534:553	optimum cooking time	534:553	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	4	76	theme	cooking	514:520	arg1	properties					522:531	their cooking properties	508:531	their cooking properties (optimum cooking time, cooking loss and swelling index)	508:587	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	4	76	theme	cooking	514:520	arg1	loss					564:567	cooking loss	556:567	cooking loss	556:567	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	4	76	theme	cooking	514:520	arg1	parameters					650:659	textural and organoleptic parameters	624:659	textural and organoleptic parameters	624:659	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	4	76	theme	cooking	514:520	arg1	index					582:586	swelling index	573:586	swelling index	573:586	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	4	76	theme	cooking	514:520	arg1	indices					615:621	whiteness and yellowness indices	590:621	whiteness and yellowness indices	590:621	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	9	77	theme	desired	1807:1813	arg1	digestibility					1835:1847	low in vitro starch digestibility	1815:1847	low in vitro starch digestibility	1815:1847	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	9	77	theme	desired	1807:1813	arg1	reduction					1887:1895	∼1.16-1.25 fold reduction	1871:1895	∼1.16-1.25 fold reduction	1871:1895	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	1	78	theme	nutritional	166:176	arg1	qualities					178:186	improved cooking/rehydrating and nutritional qualities	133:186	improved cooking/rehydrating and nutritional qualities	133:186	The present study was undertaken to develop rice-based composite pasta with improved cooking/rehydrating and nutritional qualities.
33291979	6	79	dep	increased	816:824	arg1	improved					995:1002	improved	995:1002	improved the sensory qualities (p < 0.05)	995:1035	However, reduction in WF increased the cooking loss, compromised textural and organoleptic attributes in samples with high RF content (80%); nevertheless, addition of GG and MGGF reduced these losses and improved the sensory qualities (p < 0.05).
33291979	6	79	dep	increased	816:824	arg1	reduced					970:976	reduced	970:976	reduced these losses	970:989	However, reduction in WF increased the cooking loss, compromised textural and organoleptic attributes in samples with high RF content (80%); nevertheless, addition of GG and MGGF reduced these losses and improved the sensory qualities (p < 0.05).
33291979	9	80	theme	RF + 19	1661:1667	arg1	%					1685:1685	70%RF + 19%WF + 10%MGGF + 1%GG	1658:1687	70%RF + 19%WF + 10%MGGF + 1%GG	1658:1687	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	9	80	theme	RF + 19	1661:1667	arg1	T7					1654:1655	T7	1654:1655	T7 (70%RF + 19%WF + 10%MGGF + 1%GG)	1654:1688	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	9	81	theme	in	1819:1820	arg1	digestibility					1835:1847	low in vitro starch digestibility	1815:1847	low in vitro starch digestibility	1815:1847	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	9	81	theme	in	1819:1820	arg1	reduction					1887:1895	∼1.16-1.25 fold reduction	1871:1895	∼1.16-1.25 fold reduction	1871:1895	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	5	82	theme	samples	782:788	arg1	indices					767:773	the whiteness and swelling indices	740:773	the whiteness and swelling indices of the samples	740:788	Substitution of WF by RF significantly reduced the cooking time, and improved the whiteness and swelling indices of the samples.
33291979	3	83	theme	%	423:423	arg1	WF					425:426	100% WF	420:426	100% WF	420:426	Pasta made of 100% WF served as the control (T0).
33291979	7	84	theme	cooked	1282:1287	arg1	counterparts					1289:1300	their cooked counterparts	1276:1300	their cooked counterparts	1276:1300	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	2	85	theme	screw	390:394	arg1	extruder					396:403	a single screw extruder	381:403	a single screw extruder	381:403	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	9	86	theme	starch	1828:1833	arg1	digestibility					1835:1847	low in vitro starch digestibility	1815:1847	low in vitro starch digestibility	1815:1847	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	9	86	theme	starch	1828:1833	arg1	reduction					1887:1895	∼1.16-1.25 fold reduction	1871:1895	∼1.16-1.25 fold reduction	1871:1895	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	4	87	dep	properties	522:531	arg1	time					550:553	optimum cooking time	534:553	optimum cooking time	534:553	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	4	87	dep	properties	522:531	arg1	loss					564:567	cooking loss	556:567	cooking loss	556:567	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	4	87	dep	properties	522:531	arg1	index					582:586	swelling index	573:586	swelling index	573:586	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	4	87	dep	properties	522:531	arg1	properties					522:531	their cooking properties	508:531	their cooking properties (optimum cooking time, cooking loss and swelling index)	508:587	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	7	88	theme	%	1396:1396	arg1	T3					1382:1383	T3	1382:1383	T3 (80%RF + 20%WF)	1382:1399	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	7	88	theme	%	1396:1396	arg1	WF					1397:1398	80%RF + 20%WF	1386:1398	80%RF + 20%WF	1386:1398	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	10	89	theme	suitable	1985:1992	arg1	amount					1994:1999	suitable amount	1985:1999	suitable amount of MGGF and GG	1985:2014	Thus low-cost quick-cooking/rehydrating pasta can be successfully developed by adding suitable amount of MGGF and GG into rice-wheat composite flours.
33291979	10	89	theme	suitable	1985:1992	arg1	GG					2013:2014	GG	2013:2014	GG	2013:2014	Thus low-cost quick-cooking/rehydrating pasta can be successfully developed by adding suitable amount of MGGF and GG into rice-wheat composite flours.
33291979	10	89	theme	suitable	1985:1992	arg1	MGGF					2004:2007	MGGF	2004:2007	MGGF	2004:2007	Thus low-cost quick-cooking/rehydrating pasta can be successfully developed by adding suitable amount of MGGF and GG into rice-wheat composite flours.
33291979	6	90	from	reduction	800:808	arg1	WF					813:814	WF	813:814	WF	813:814	However, reduction in WF increased the cooking loss, compromised textural and organoleptic attributes in samples with high RF content (80%); nevertheless, addition of GG and MGGF reduced these losses and improved the sensory qualities (p < 0.05).
33291979	4	91	theme	whiteness	590:598	arg1	indices					615:621	whiteness and yellowness indices	590:621	whiteness and yellowness indices	590:621	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	4	91	theme	whiteness	590:598	arg1	properties					522:531	their cooking properties	508:531	their cooking properties (optimum cooking time, cooking loss and swelling index)	508:587	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	8	92	theme	rehydrated	1500:1509	arg1	samples					1511:1517	the rehydrated samples	1496:1517	the rehydrated samples	1496:1517	Adhesiveness of the rehydrated samples was lesser than their cooked counterparts, while vice versa was observed in case of their instrumental firmness/hardness.
33291979	5	93	theme	swelling	758:765	arg1	indices					767:773	the whiteness and swelling indices	740:773	the whiteness and swelling indices of the samples	740:788	Substitution of WF by RF significantly reduced the cooking time, and improved the whiteness and swelling indices of the samples.
33291979	7	94	theme	%	1388:1388	arg1	T3					1382:1383	T3	1382:1383	T3 (80%RF + 20%WF)	1382:1399	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	7	94	theme	%	1388:1388	arg1	WF					1397:1398	80%RF + 20%WF	1386:1398	80%RF + 20%WF	1386:1398	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	7	95	theme	optimal	1095:1101	arg1	time					1111:1114	the optimal cooking time	1091:1114	the optimal cooking time	1091:1114	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	2	96	theme	gum	346:348	arg1	proportions					229:239	different proportions	219:239	different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%)	219:359	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	6	97	theme	organoleptic	869:880	arg1	attributes					882:891	textural and organoleptic attributes	856:891	textural and organoleptic attributes in samples with high RF content (80%)	856:929	However, reduction in WF increased the cooking loss, compromised textural and organoleptic attributes in samples with high RF content (80%); nevertheless, addition of GG and MGGF reduced these losses and improved the sensory qualities (p < 0.05).
33291979	4	98	theme	swelling	573:580	arg1	index					582:586	swelling index	573:586	swelling index	573:586	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	4	98	theme	swelling	573:580	arg1	properties					522:531	their cooking properties	508:531	their cooking properties (optimum cooking time, cooking loss and swelling index)	508:587	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	2	99	contain	having	212:217	arg1	samples					195:201	Pasta samples	189:201	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%)	189:359	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	2	99	contain	having	212:217	arg2	proportions					229:239	different proportions	219:239	different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%)	219:359	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	2	99	contain	having	212:217	arg1	T1-T10					204:209	T1-T10	204:209	T1-T10	204:209	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	7	100	theme	rice-based	1073:1082	arg1	pasta					1084:1088	rice-based pasta	1073:1088	rice-based pasta	1073:1088	Irrespective of the composition of rice-based pasta, the optimal cooking time ranged within 2-4 min (unlike T0) and these samples could also be rehydrated in hot water within a short span of 5 min to attain textural qualities at par with their cooked counterparts and with sensorial scores well above the limit of acceptable range (≥5) (except T3 (80%RF + 20%WF)); nonetheless the cooked ones led to higher preference from the sensory panel.
33291979	2	101	theme	Pasta	189:193	arg1	samples					195:201	Pasta samples	189:201	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%)	189:359	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	2	101	theme	Pasta	189:193	arg1	T1-T10					204:209	T1-T10	204:209	T1-T10	204:209	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	6	102	with	samples	896:902	arg1	content					917:923	high RF content	909:923	high RF content (80%)	909:929	However, reduction in WF increased the cooking loss, compromised textural and organoleptic attributes in samples with high RF content (80%); nevertheless, addition of GG and MGGF reduced these losses and improved the sensory qualities (p < 0.05).
33291979	6	102	with	samples	896:902	arg1	%					928:928	80%	926:928	80%	926:928	However, reduction in WF increased the cooking loss, compromised textural and organoleptic attributes in samples with high RF content (80%); nevertheless, addition of GG and MGGF reduced these losses and improved the sensory qualities (p < 0.05).
33291979	10	103	theme	composite	2032:2040	arg1	flours					2042:2047	rice-wheat composite flours	2021:2047	rice-wheat composite flours	2021:2047	Thus low-cost quick-cooking/rehydrating pasta can be successfully developed by adding suitable amount of MGGF and GG into rice-wheat composite flours.
33291979	2	104	theme	different	219:227	arg1	proportions					229:239	different proportions	219:239	different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%)	219:359	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	2	105	theme	flour	275:279	arg1	proportions					229:239	different proportions	219:239	different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%)	219:359	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
33291979	9	106	theme	%	1708:1708	arg1	%					1724:1724	80%RF + 13%WF + 5%MGGF + 2%GG	1698:1726	80%RF + 13%WF + 5%MGGF + 2%GG	1698:1726	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	9	106	theme	%	1708:1708	arg1	T9					1694:1695	T9	1694:1695	T9 (80%RF + 13%WF + 5%MGGF + 2%GG)	1694:1727	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	4	107	theme	yellowness	604:613	arg1	indices					615:621	whiteness and yellowness indices	590:621	whiteness and yellowness indices	590:621	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	4	107	theme	yellowness	604:613	arg1	properties					522:531	their cooking properties	508:531	their cooking properties (optimum cooking time, cooking loss and swelling index)	508:587	The cooked or rehydrated samples were evaluated for their cooking properties (optimum cooking time, cooking loss and swelling index), whiteness and yellowness indices, textural and organoleptic parameters.
33291979	9	108	dep	in	1819:1820	arg1	vitro					1822:1826	vitro	1822:1826	vitro	1822:1826	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	9	109	theme	%	1715:1715	arg1	%					1724:1724	80%RF + 13%WF + 5%MGGF + 2%GG	1698:1726	80%RF + 13%WF + 5%MGGF + 2%GG	1698:1726	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	9	109	theme	%	1715:1715	arg1	T9					1694:1695	T9	1694:1695	T9 (80%RF + 13%WF + 5%MGGF + 2%GG)	1694:1727	Among these, T7 (70%RF + 19%WF + 10%MGGF + 1%GG) and T9 (80%RF + 13%WF + 5%MGGF + 2%GG) exhibited high protein content (∼1.17-1.29 times higher than T0) and the most desired low in vitro starch digestibility and calorific values (∼1.16-1.25 fold reduction).
33291979	2	110	theme	gram	308:311	arg1	flour					313:317	malted green gram flour	295:317	malted green gram flour (MGGF, 5 and 10%)	295:335	Pasta samples (T1-T10) having different proportions of rice flour (RF, 60-80%), wheat flour (WF, 40-12%), malted green gram flour (MGGF, 5 and 10%) and guar gum (GG, 1-3%) were prepared using a single screw extruder.
34973658	6	0	theme	temperature	1112:1122	arg1	effects					1069:1075	The effects	1065:1075	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites	1065:1223	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites were investigated.
34973658	0	1	theme	blue	175:178	arg1	methylene					165:173	methylene blue	165:178	methylene blue	165:178	Barium alginate as a skeleton coating graphene oxide and bentonite-derived composites: Excellent adsorbent based on predictive design for the enhanced adsorption of methylene blue.
34973658	1	2	theme	carrier	265:271	arg1	material					273:280	a carrier material	263:280	a carrier material	263:280	The phenomenon that calcium alginate does not exhibit high adsorption capacity as a carrier material has not been reasonably explained or solved.
34973658	1	2	theme	carrier	265:271	arg1	capacity					251:258	high adsorption capacity	235:258	high adsorption capacity	235:258	The phenomenon that calcium alginate does not exhibit high adsorption capacity as a carrier material has not been reasonably explained or solved.
34973658	6	3	from	effects	1069:1075	arg1	performance					1196:1206	the adsorption performance	1181:1206	the adsorption performance of BA composites	1181:1223	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites were investigated.
34973658	6	4	theme	concentration	1164:1176	arg1	effects					1069:1075	The effects	1065:1075	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites	1065:1223	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites were investigated.
34973658	5	5	dep	transform	1032:1040	arg1	infrared					1042:1049	infrared	1042:1049	transform infrared spectroscopy	1032:1062	The structure and morphology of the prepared adsorbents were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, thermogravimetry and Fourier transform infrared spectroscopy.
34973658	9	6	theme	sodium	1499:1504	arg1	alginate					1506:1513	sodium alginate	1499:1513	sodium alginate	1499:1513	Benefiting from the strong interaction between bentonite, sodium alginate and Ba2+, the 3D-BA hydrogel showed higher thermal stability and better adsorption efficiency than other materials.
34973658	6	7	theme	initial	1152:1158	arg1	concentration					1164:1176	initial dye concentration	1152:1176	initial dye concentration	1152:1176	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites were investigated.
34973658	10	8	theme	Elovich	1635:1641	arg1	model					1651:1655	The Elovich kinetic model	1631:1655	The Elovich kinetic model	1631:1655	The Elovich kinetic model and Sips equation can appropriately describe the adsorption process.
34973658	0	9	theme	predictive	116:125	arg1	design					127:132	predictive design	116:132	predictive design for the enhanced adsorption of methylene blue	116:178	Barium alginate as a skeleton coating graphene oxide and bentonite-derived composites: Excellent adsorbent based on predictive design for the enhanced adsorption of methylene blue.
34973658	1	10	theme	calcium	201:207	arg1	alginate					209:216	calcium alginate	201:216	calcium alginate	201:216	The phenomenon that calcium alginate does not exhibit high adsorption capacity as a carrier material has not been reasonably explained or solved.
34973658	5	11	theme	adsorbents	906:915	arg1	morphology					879:888	morphology	879:888	morphology	879:888	The structure and morphology of the prepared adsorbents were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, thermogravimetry and Fourier transform infrared spectroscopy.
34973658	5	11	theme	adsorbents	906:915	arg1	structure					865:873	structure	865:873	structure	865:873	The structure and morphology of the prepared adsorbents were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, thermogravimetry and Fourier transform infrared spectroscopy.
34973658	10	12	theme	Sips	1661:1664	arg1	equation					1666:1673	Sips equation	1661:1673	Sips equation	1661:1673	The Elovich kinetic model and Sips equation can appropriately describe the adsorption process.
34973658	0	13	theme	enhanced	142:149	arg1	adsorption					151:160	the enhanced adsorption	138:160	the enhanced adsorption of methylene blue	138:178	Barium alginate as a skeleton coating graphene oxide and bentonite-derived composites: Excellent adsorbent based on predictive design for the enhanced adsorption of methylene blue.
34973658	9	14	theme	3D-BA	1529:1533	arg1	hydrogel					1535:1542	the 3D-BA hydrogel	1525:1542	the 3D-BA hydrogel	1525:1542	Benefiting from the strong interaction between bentonite, sodium alginate and Ba2+, the 3D-BA hydrogel showed higher thermal stability and better adsorption efficiency than other materials.
34973658	4	15	dep	three-dimensional	707:723	arg1	3D					726:727	3D	726:727	3D	726:727	Barium alginate/graphene oxide (BA/GO) membranes and three-dimensional (3D) barium alginate-bentonite-graphene oxide derived (3D-BA) hydrogels were prepared by vacuum freeze-drying to remove methylene blue.
34973658	6	16	theme	BA	1211:1212	arg1	composites					1214:1223	BA composites	1211:1223	BA composites	1211:1223	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites were investigated.
34973658	4	17	theme	alginate/graphene	661:677	arg1	oxide					679:683	Barium alginate/graphene oxide	654:683	Barium alginate/graphene oxide (BA/GO) membranes	654:701	Barium alginate/graphene oxide (BA/GO) membranes and three-dimensional (3D) barium alginate-bentonite-graphene oxide derived (3D-BA) hydrogels were prepared by vacuum freeze-drying to remove methylene blue.
34973658	4	17	theme	alginate/graphene	661:677	arg1	BA/GO					686:690	BA/GO	686:690	BA/GO	686:690	Barium alginate/graphene oxide (BA/GO) membranes and three-dimensional (3D) barium alginate-bentonite-graphene oxide derived (3D-BA) hydrogels were prepared by vacuum freeze-drying to remove methylene blue.
34973658	7	18	theme	adsorption	1248:1257	arg1	capacities					1259:1268	The adsorption capacities	1244:1268	The adsorption capacities of the BA/GO and 3D-BA materials	1244:1301	The adsorption capacities of the BA/GO and 3D-BA materials were 1011.3 and 710.3 mg/g, respectively.
34973658	7	18	theme	adsorption	1248:1257	arg1	1011.3					1308:1313	1011.3	1308:1313	1011.3	1308:1313	The adsorption capacities of the BA/GO and 3D-BA materials were 1011.3 and 710.3 mg/g, respectively.
34973658	9	19	theme	thermal	1558:1564	arg1	stability					1566:1574	higher thermal stability	1551:1574	higher thermal stability	1551:1574	Benefiting from the strong interaction between bentonite, sodium alginate and Ba2+, the 3D-BA hydrogel showed higher thermal stability and better adsorption efficiency than other materials.
34973658	2	20	theme	units	467:471	arg1	degree					418:423	the binding degree	406:423	the binding degree of the α-l-guluronate and β-d-mannuronate units	406:471	In this paper, a new viewpoint that the orbital energy level of metal ions and the binding degree of the α-l-guluronate and β-d-mannuronate units affect the adsorption performance of the composite was innovatively proposed.
34973658	2	20	theme	units	467:471	arg1	level					382:386	the orbital energy level	363:386	the orbital energy level of metal ions	363:400	In this paper, a new viewpoint that the orbital energy level of metal ions and the binding degree of the α-l-guluronate and β-d-mannuronate units affect the adsorption performance of the composite was innovatively proposed.
34973658	8	21	theme	BA/GO	1349:1353	arg1	membrane					1355:1362	The BA/GO membrane	1345:1362	The BA/GO membrane	1345:1362	The BA/GO membrane exhibited stable filtration performance against high concentrations of dyes.
34973658	4	22	theme	alginate-bentonite-graphene	737:763	arg1	oxide					765:769	barium alginate-bentonite-graphene oxide	730:769	three-dimensional (3D) barium alginate-bentonite-graphene oxide derived (3D-BA) hydrogels	707:795	Barium alginate/graphene oxide (BA/GO) membranes and three-dimensional (3D) barium alginate-bentonite-graphene oxide derived (3D-BA) hydrogels were prepared by vacuum freeze-drying to remove methylene blue.
34973658	4	22	theme	alginate-bentonite-graphene	737:763	arg1	3D-BA					780:784	3D-BA	780:784	3D-BA	780:784	Barium alginate/graphene oxide (BA/GO) membranes and three-dimensional (3D) barium alginate-bentonite-graphene oxide derived (3D-BA) hydrogels were prepared by vacuum freeze-drying to remove methylene blue.
34973658	5	23	theme	X-ray	969:973	arg1	spectroscopy					989:1000	X-ray photoelectron spectroscopy	969:1000	X-ray photoelectron spectroscopy	969:1000	The structure and morphology of the prepared adsorbents were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, thermogravimetry and Fourier transform infrared spectroscopy.
34973658	2	24	dep	viewpoint	348:356	arg1	affect					473:478	affect	473:478	affect the adsorption performance of the composite	473:522	In this paper, a new viewpoint that the orbital energy level of metal ions and the binding degree of the α-l-guluronate and β-d-mannuronate units affect the adsorption performance of the composite was innovatively proposed.
34973658	2	25	theme	energy	375:380	arg1	level					382:386	the orbital energy level	363:386	the orbital energy level of metal ions	363:400	In this paper, a new viewpoint that the orbital energy level of metal ions and the binding degree of the α-l-guluronate and β-d-mannuronate units affect the adsorption performance of the composite was innovatively proposed.
34973658	6	26	theme	doping	1098:1103	arg1	ratio					1105:1109	doping ratio	1098:1109	doping ratio	1098:1109	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites were investigated.
34973658	0	27	theme	Barium	0:5	arg1	alginate					7:14	Barium alginate	0:14	Barium alginate as a skeleton coating graphene oxide and bentonite-derived composites: Excellent adsorbent based on predictive design for the enhanced adsorption of methylene blue.	0:179	Barium alginate as a skeleton coating graphene oxide and bentonite-derived composites: Excellent adsorbent based on predictive design for the enhanced adsorption of methylene blue.
34973658	5	28	theme	scanning	939:946	arg1	microscopy					957:966	scanning electron microscopy	939:966	scanning electron microscopy	939:966	The structure and morphology of the prepared adsorbents were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, thermogravimetry and Fourier transform infrared spectroscopy.
34973658	6	29	theme	dosage	1090:1095	arg1	effects					1069:1075	The effects	1065:1075	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites	1065:1223	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites were investigated.
34973658	2	30	theme	ions	397:400	arg1	degree					418:423	the binding degree	406:423	the binding degree of the α-l-guluronate and β-d-mannuronate units	406:471	In this paper, a new viewpoint that the orbital energy level of metal ions and the binding degree of the α-l-guluronate and β-d-mannuronate units affect the adsorption performance of the composite was innovatively proposed.
34973658	2	30	theme	ions	397:400	arg1	level					382:386	the orbital energy level	363:386	the orbital energy level of metal ions	363:400	In this paper, a new viewpoint that the orbital energy level of metal ions and the binding degree of the α-l-guluronate and β-d-mannuronate units affect the adsorption performance of the composite was innovatively proposed.
34973658	3	31	theme	calcium	623:629	arg1	alginate					631:638	replacing calcium alginate	613:638	replacing calcium alginate	613:638	Taking barium alginate (BA) as an example, the possibility of replacing calcium alginate is discussed.
34973658	8	32	theme	dyes	1435:1438	arg1	concentrations					1417:1430	high concentrations	1412:1430	high concentrations of dyes	1412:1438	The BA/GO membrane exhibited stable filtration performance against high concentrations of dyes.
34973658	11	33	theme	barium	1748:1753	arg1	alginate					1755:1762	barium alginate	1748:1762	barium alginate	1748:1762	The results show that barium alginate is a better carrier material than calcium alginate.
34973658	11	33	theme	barium	1748:1753	arg1	material					1784:1791	a better carrier material	1767:1791	a better carrier material than calcium alginate	1767:1813	The results show that barium alginate is a better carrier material than calcium alginate.
34973658	4	34	theme	vacuum	814:819	arg1	freeze-drying					821:833	vacuum freeze-drying	814:833	vacuum freeze-drying	814:833	Barium alginate/graphene oxide (BA/GO) membranes and three-dimensional (3D) barium alginate-bentonite-graphene oxide derived (3D-BA) hydrogels were prepared by vacuum freeze-drying to remove methylene blue.
34973658	0	35	theme	graphene	38:45	arg1	oxide					47:51	graphene oxide	38:51	graphene oxide	38:51	Barium alginate as a skeleton coating graphene oxide and bentonite-derived composites: Excellent adsorbent based on predictive design for the enhanced adsorption of methylene blue.
34973658	0	36	link	bentonite-derived	57:73	arg1	composites					75:84	bentonite-derived composites	57:84	bentonite-derived composites	57:84	Barium alginate as a skeleton coating graphene oxide and bentonite-derived composites: Excellent adsorbent based on predictive design for the enhanced adsorption of methylene blue.
34973658	6	37	theme	pH	1139:1140	arg1	value					1142:1146	pH value	1139:1146	pH value	1139:1146	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites were investigated.
34973658	7	38	theme	3D-BA	1287:1291	arg1	materials					1293:1301	the BA/GO and 3D-BA materials	1273:1301	materials	1293:1301	The adsorption capacities of the BA/GO and 3D-BA materials were 1011.3 and 710.3 mg/g, respectively.
34973658	11	39	theme	better	1769:1774	arg1	alginate					1755:1762	barium alginate	1748:1762	barium alginate	1748:1762	The results show that barium alginate is a better carrier material than calcium alginate.
34973658	11	39	theme	better	1769:1774	arg1	material					1784:1791	a better carrier material	1767:1791	a better carrier material than calcium alginate	1767:1813	The results show that barium alginate is a better carrier material than calcium alginate.
34973658	1	40	theme	high	235:238	arg1	material					273:280	a carrier material	263:280	a carrier material	263:280	The phenomenon that calcium alginate does not exhibit high adsorption capacity as a carrier material has not been reasonably explained or solved.
34973658	1	40	theme	high	235:238	arg1	capacity					251:258	high adsorption capacity	235:258	high adsorption capacity	235:258	The phenomenon that calcium alginate does not exhibit high adsorption capacity as a carrier material has not been reasonably explained or solved.
34973658	7	41	theme	BA/GO	1277:1281	arg1	materials					1293:1301	the BA/GO and 3D-BA materials	1273:1301	materials	1293:1301	The adsorption capacities of the BA/GO and 3D-BA materials were 1011.3 and 710.3 mg/g, respectively.
34973658	9	42	theme	strong	1461:1466	arg1	interaction					1468:1478	the strong interaction	1457:1478	the strong interaction between bentonite, sodium alginate and Ba2+	1457:1522	Benefiting from the strong interaction between bentonite, sodium alginate and Ba2+, the 3D-BA hydrogel showed higher thermal stability and better adsorption efficiency than other materials.
34973658	6	43	theme	adsorption	1185:1194	arg1	performance					1196:1206	the adsorption performance	1181:1206	the adsorption performance of BA composites	1181:1223	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites were investigated.
34973658	7	44	theme	materials	1293:1301	arg1	capacities					1259:1268	The adsorption capacities	1244:1268	The adsorption capacities of the BA/GO and 3D-BA materials	1244:1301	The adsorption capacities of the BA/GO and 3D-BA materials were 1011.3 and 710.3 mg/g, respectively.
34973658	7	44	theme	materials	1293:1301	arg1	1011.3					1308:1313	1011.3	1308:1313	1011.3	1308:1313	The adsorption capacities of the BA/GO and 3D-BA materials were 1011.3 and 710.3 mg/g, respectively.
34973658	3	45	theme	barium	558:563	arg1	example					585:591	an example	582:591	an example	582:591	Taking barium alginate (BA) as an example, the possibility of replacing calcium alginate is discussed.
34973658	3	45	theme	barium	558:563	arg1	BA					575:576	BA	575:576	BA	575:576	Taking barium alginate (BA) as an example, the possibility of replacing calcium alginate is discussed.
34973658	3	45	theme	barium	558:563	arg1	alginate					565:572	barium alginate	558:572	barium alginate (BA)	558:577	Taking barium alginate (BA) as an example, the possibility of replacing calcium alginate is discussed.
34973658	6	46	theme	dye	1160:1162	arg1	concentration					1164:1176	initial dye concentration	1152:1176	initial dye concentration	1152:1176	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites were investigated.
34973658	10	47	theme	kinetic	1643:1649	arg1	model					1651:1655	The Elovich kinetic model	1631:1655	The Elovich kinetic model	1631:1655	The Elovich kinetic model and Sips equation can appropriately describe the adsorption process.
34973658	5	48	dep	Fourier	1024:1030	arg1	transform					1032:1040	transform	1032:1040	transform infrared spectroscopy	1032:1062	The structure and morphology of the prepared adsorbents were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, thermogravimetry and Fourier transform infrared spectroscopy.
34973658	2	49	theme	composite	514:522	arg1	performance					495:505	the adsorption performance	480:505	the adsorption performance of the composite	480:522	In this paper, a new viewpoint that the orbital energy level of metal ions and the binding degree of the α-l-guluronate and β-d-mannuronate units affect the adsorption performance of the composite was innovatively proposed.
34973658	5	50	theme	prepared	897:904	arg1	adsorbents					906:915	the prepared adsorbents	893:915	the prepared adsorbents	893:915	The structure and morphology of the prepared adsorbents were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, thermogravimetry and Fourier transform infrared spectroscopy.
34973658	0	51	theme	methylene	165:173	arg1	adsorption					151:160	the enhanced adsorption	138:160	the enhanced adsorption of methylene blue	138:178	Barium alginate as a skeleton coating graphene oxide and bentonite-derived composites: Excellent adsorbent based on predictive design for the enhanced adsorption of methylene blue.
34973658	6	52	theme	composites	1214:1223	arg1	performance					1196:1206	the adsorption performance	1181:1206	the adsorption performance of BA composites	1181:1223	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites were investigated.
34973658	0	53	theme	bentonite-derived	57:73	arg1	composites					75:84	bentonite-derived composites	57:84	bentonite-derived composites	57:84	Barium alginate as a skeleton coating graphene oxide and bentonite-derived composites: Excellent adsorbent based on predictive design for the enhanced adsorption of methylene blue.
34973658	9	54	theme	higher	1551:1556	arg1	stability					1566:1574	higher thermal stability	1551:1574	higher thermal stability	1551:1574	Benefiting from the strong interaction between bentonite, sodium alginate and Ba2+, the 3D-BA hydrogel showed higher thermal stability and better adsorption efficiency than other materials.
34973658	4	55	theme	Barium	654:659	arg1	oxide					679:683	Barium alginate/graphene oxide	654:683	Barium alginate/graphene oxide (BA/GO) membranes	654:701	Barium alginate/graphene oxide (BA/GO) membranes and three-dimensional (3D) barium alginate-bentonite-graphene oxide derived (3D-BA) hydrogels were prepared by vacuum freeze-drying to remove methylene blue.
34973658	4	55	theme	Barium	654:659	arg1	BA/GO					686:690	BA/GO	686:690	BA/GO	686:690	Barium alginate/graphene oxide (BA/GO) membranes and three-dimensional (3D) barium alginate-bentonite-graphene oxide derived (3D-BA) hydrogels were prepared by vacuum freeze-drying to remove methylene blue.
34973658	11	56	theme	calcium	1798:1804	arg1	alginate					1806:1813	calcium alginate	1798:1813	calcium alginate	1798:1813	The results show that barium alginate is a better carrier material than calcium alginate.
34973658	2	57	theme	β-d-mannuronate	451:465	arg1	units					467:471	the α-l-guluronate and β-d-mannuronate units	428:471	the α-l-guluronate and β-d-mannuronate units	428:471	In this paper, a new viewpoint that the orbital energy level of metal ions and the binding degree of the α-l-guluronate and β-d-mannuronate units affect the adsorption performance of the composite was innovatively proposed.
34973658	4	58	theme	oxide	679:683	arg1	membranes					693:701	Barium alginate/graphene oxide (BA/GO) membranes	654:701	Barium alginate/graphene oxide (BA/GO) membranes	654:701	Barium alginate/graphene oxide (BA/GO) membranes and three-dimensional (3D) barium alginate-bentonite-graphene oxide derived (3D-BA) hydrogels were prepared by vacuum freeze-drying to remove methylene blue.
34973658	9	59	theme	better	1580:1585	arg1	efficiency					1598:1607	better adsorption efficiency	1580:1607	better adsorption efficiency than other materials	1580:1628	Benefiting from the strong interaction between bentonite, sodium alginate and Ba2+, the 3D-BA hydrogel showed higher thermal stability and better adsorption efficiency than other materials.
34973658	9	60	theme	adsorption	1587:1596	arg1	efficiency					1598:1607	better adsorption efficiency	1580:1607	better adsorption efficiency than other materials	1580:1628	Benefiting from the strong interaction between bentonite, sodium alginate and Ba2+, the 3D-BA hydrogel showed higher thermal stability and better adsorption efficiency than other materials.
34973658	4	61	theme	barium	730:735	arg1	oxide					765:769	barium alginate-bentonite-graphene oxide	730:769	three-dimensional (3D) barium alginate-bentonite-graphene oxide derived (3D-BA) hydrogels	707:795	Barium alginate/graphene oxide (BA/GO) membranes and three-dimensional (3D) barium alginate-bentonite-graphene oxide derived (3D-BA) hydrogels were prepared by vacuum freeze-drying to remove methylene blue.
34973658	4	61	theme	barium	730:735	arg1	3D-BA					780:784	3D-BA	780:784	3D-BA	780:784	Barium alginate/graphene oxide (BA/GO) membranes and three-dimensional (3D) barium alginate-bentonite-graphene oxide derived (3D-BA) hydrogels were prepared by vacuum freeze-drying to remove methylene blue.
34973658	6	62	theme	time	1133:1136	arg1	effects					1069:1075	The effects	1065:1075	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites	1065:1223	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites were investigated.
34973658	5	63	theme	photoelectron	975:987	arg1	spectroscopy					989:1000	X-ray photoelectron spectroscopy	969:1000	X-ray photoelectron spectroscopy	969:1000	The structure and morphology of the prepared adsorbents were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, thermogravimetry and Fourier transform infrared spectroscopy.
34973658	6	64	theme	ratio	1105:1109	arg1	effects					1069:1075	The effects	1065:1075	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites	1065:1223	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites were investigated.
34973658	2	65	theme	adsorption	484:493	arg1	performance					495:505	the adsorption performance	480:505	the adsorption performance of the composite	480:522	In this paper, a new viewpoint that the orbital energy level of metal ions and the binding degree of the α-l-guluronate and β-d-mannuronate units affect the adsorption performance of the composite was innovatively proposed.
34973658	8	66	theme	stable	1374:1379	arg1	performance					1392:1402	stable filtration performance	1374:1402	stable filtration performance	1374:1402	The BA/GO membrane exhibited stable filtration performance against high concentrations of dyes.
34973658	10	67	theme	adsorption	1706:1715	arg1	process					1717:1723	the adsorption process	1702:1723	the adsorption process	1702:1723	The Elovich kinetic model and Sips equation can appropriately describe the adsorption process.
34973658	9	68	theme	other	1614:1618	arg1	materials					1620:1628	other materials	1614:1628	other materials	1614:1628	Benefiting from the strong interaction between bentonite, sodium alginate and Ba2+, the 3D-BA hydrogel showed higher thermal stability and better adsorption efficiency than other materials.
34973658	2	69	theme	binding	410:416	arg1	degree					418:423	the binding degree	406:423	the binding degree of the α-l-guluronate and β-d-mannuronate units	406:471	In this paper, a new viewpoint that the orbital energy level of metal ions and the binding degree of the α-l-guluronate and β-d-mannuronate units affect the adsorption performance of the composite was innovatively proposed.
34973658	4	70	theme	three-dimensional	707:723	arg1	hydrogels					787:795	three-dimensional (3D) barium alginate-bentonite-graphene oxide derived (3D-BA) hydrogels	707:795	three-dimensional (3D) barium alginate-bentonite-graphene oxide derived (3D-BA) hydrogels	707:795	Barium alginate/graphene oxide (BA/GO) membranes and three-dimensional (3D) barium alginate-bentonite-graphene oxide derived (3D-BA) hydrogels were prepared by vacuum freeze-drying to remove methylene blue.
34973658	5	71	theme	electron	948:955	arg1	microscopy					957:966	scanning electron microscopy	939:966	scanning electron microscopy	939:966	The structure and morphology of the prepared adsorbents were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, thermogravimetry and Fourier transform infrared spectroscopy.
34973658	6	72	theme	adsorbent	1080:1088	arg1	dosage					1090:1095	adsorbent dosage	1080:1095	adsorbent dosage	1080:1095	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites were investigated.
34973658	8	73	theme	filtration	1381:1390	arg1	performance					1392:1402	stable filtration performance	1374:1402	stable filtration performance	1374:1402	The BA/GO membrane exhibited stable filtration performance against high concentrations of dyes.
34973658	3	74	theme	replacing	613:621	arg1	alginate					631:638	replacing calcium alginate	613:638	replacing calcium alginate	613:638	Taking barium alginate (BA) as an example, the possibility of replacing calcium alginate is discussed.
34973658	8	75	theme	high	1412:1415	arg1	concentrations					1417:1430	high concentrations	1412:1430	high concentrations of dyes	1412:1438	The BA/GO membrane exhibited stable filtration performance against high concentrations of dyes.
34973658	2	76	theme	metal	391:395	arg1	ions					397:400	metal ions	391:400	metal ions	391:400	In this paper, a new viewpoint that the orbital energy level of metal ions and the binding degree of the α-l-guluronate and β-d-mannuronate units affect the adsorption performance of the composite was innovatively proposed.
34973658	2	77	theme	α-l-guluronate	432:445	arg1	units					467:471	the α-l-guluronate and β-d-mannuronate units	428:471	the α-l-guluronate and β-d-mannuronate units	428:471	In this paper, a new viewpoint that the orbital energy level of metal ions and the binding degree of the α-l-guluronate and β-d-mannuronate units affect the adsorption performance of the composite was innovatively proposed.
34973658	5	78	dep	structure	865:873	arg1	The					861:863	The	861:863	The	861:863	The structure and morphology of the prepared adsorbents were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, thermogravimetry and Fourier transform infrared spectroscopy.
34973658	6	79	theme	value	1142:1146	arg1	effects					1069:1075	The effects	1065:1075	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites	1065:1223	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites were investigated.
34973658	11	80	theme	carrier	1776:1782	arg1	alginate					1755:1762	barium alginate	1748:1762	barium alginate	1748:1762	The results show that barium alginate is a better carrier material than calcium alginate.
34973658	11	80	theme	carrier	1776:1782	arg1	material					1784:1791	a better carrier material	1767:1791	a better carrier material than calcium alginate	1767:1813	The results show that barium alginate is a better carrier material than calcium alginate.
34973658	4	81	theme	oxide	765:769	arg1	hydrogels					787:795	three-dimensional (3D) barium alginate-bentonite-graphene oxide derived (3D-BA) hydrogels	707:795	three-dimensional (3D) barium alginate-bentonite-graphene oxide derived (3D-BA) hydrogels	707:795	Barium alginate/graphene oxide (BA/GO) membranes and three-dimensional (3D) barium alginate-bentonite-graphene oxide derived (3D-BA) hydrogels were prepared by vacuum freeze-drying to remove methylene blue.
34973658	3	82	theme	alginate	631:638	arg1	possibility					598:608	the possibility	594:608	the possibility of replacing calcium alginate	594:638	Taking barium alginate (BA) as an example, the possibility of replacing calcium alginate is discussed.
34973658	0	83	dep	alginate	7:14	arg1	adsorbent					97:105	adsorbent	97:105	adsorbent	97:105	Barium alginate as a skeleton coating graphene oxide and bentonite-derived composites: Excellent adsorbent based on predictive design for the enhanced adsorption of methylene blue.
34973658	6	84	theme	contact	1125:1131	arg1	time					1133:1136	contact time	1125:1136	contact time	1125:1136	The effects of adsorbent dosage, doping ratio, temperature, contact time, pH value and initial dye concentration on the adsorption performance of BA composites were investigated.
34973658	2	85	theme	orbital	367:373	arg1	level					382:386	the orbital energy level	363:386	the orbital energy level of metal ions	363:400	In this paper, a new viewpoint that the orbital energy level of metal ions and the binding degree of the α-l-guluronate and β-d-mannuronate units affect the adsorption performance of the composite was innovatively proposed.
34973658	2	86	theme	new	344:346	arg1	viewpoint					348:356	a new viewpoint	342:356	a new viewpoint that the orbital energy level of metal ions and the binding degree of the α-l-guluronate and β-d-mannuronate units affect the adsorption performance of the composite	342:522	In this paper, a new viewpoint that the orbital energy level of metal ions and the binding degree of the α-l-guluronate and β-d-mannuronate units affect the adsorption performance of the composite was innovatively proposed.
34973658	1	87	theme	adsorption	240:249	arg1	material					273:280	a carrier material	263:280	a carrier material	263:280	The phenomenon that calcium alginate does not exhibit high adsorption capacity as a carrier material has not been reasonably explained or solved.
34973658	1	87	theme	adsorption	240:249	arg1	capacity					251:258	high adsorption capacity	235:258	high adsorption capacity	235:258	The phenomenon that calcium alginate does not exhibit high adsorption capacity as a carrier material has not been reasonably explained or solved.
33370693	0	0	theme	light	105:109	arg1	irradiation					111:121	visible light irradiation	97:121	visible light irradiation	97:121	Photocatalytic graphitic carbon nitride-chitosan composites for pathogenic biofilm control under visible light irradiation.
33370693	6	1	theme	bacterial	1125:1133	arg1	species					1135:1141	multiple bacterial species	1116:1141	multiple bacterial species	1116:1141	Moreover, the composites exhibited excellent performance for inhibiting biofilm development in urine, highlighting the promise for inactivating environmental biofilms developed from multiple bacterial species.
33370693	2	2	theme	building	424:431	arg1	applications					433:444	environmental, food, biomedical, and building applications	387:444	environmental, food, biomedical, and building applications	387:444	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications.
33370693	0	3	theme	visible	97:103	arg1	irradiation					111:121	visible light irradiation	97:121	visible light irradiation	97:121	Photocatalytic graphitic carbon nitride-chitosan composites for pathogenic biofilm control under visible light irradiation.
33370693	3	4	theme	O157	620:623	arg1	eradication					489:499	eradication	489:499	eradication	489:499	The broad-spectrum biofilm inhibition and eradication of the photocatalytic composites against Staphylococcus epidermidis, Pseudomonas aeruginosa PAO1, and Escherichia coli O157: H7 under visible light irradiation were demonstrated.
33370693	3	4	theme	O157	620:623	arg1	inhibition					474:483	The broad-spectrum biofilm inhibition	447:483	The broad-spectrum biofilm inhibition	447:483	The broad-spectrum biofilm inhibition and eradication of the photocatalytic composites against Staphylococcus epidermidis, Pseudomonas aeruginosa PAO1, and Escherichia coli O157: H7 under visible light irradiation were demonstrated.
33370693	6	5	theme	inhibiting	995:1004	arg1	development					1014:1024	inhibiting biofilm development	995:1024	inhibiting biofilm development	995:1024	Moreover, the composites exhibited excellent performance for inhibiting biofilm development in urine, highlighting the promise for inactivating environmental biofilms developed from multiple bacterial species.
33370693	4	6	theme	polymeric	694:702	arg1	substances					704:713	Extracellular polymeric substances	680:713	Extracellular polymeric substances in Escherichia coli O157: H7 biofilms	680:751	Extracellular polymeric substances in Escherichia coli O157: H7 biofilms were most resistant to photocatalytic oxidation, which led to reduced performance for biofilm removal.
33370693	4	7	dep	Escherichia	718:728	arg1	coli					730:733	coli	730:733	coli	730:733	Extracellular polymeric substances in Escherichia coli O157: H7 biofilms were most resistant to photocatalytic oxidation, which led to reduced performance for biofilm removal.
33370693	2	8	theme	pathogenic	363:372	arg1	biofilms					374:381	pathogenic biofilms	363:381	pathogenic biofilms for environmental, food, biomedical, and building applications	363:444	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications.
33370693	4	9	theme	Escherichia	718:728	arg1	O157					735:738	Escherichia coli O157	718:738	Escherichia coli O157: H7 biofilms	718:751	Extracellular polymeric substances in Escherichia coli O157: H7 biofilms were most resistant to photocatalytic oxidation, which led to reduced performance for biofilm removal.
33370693	4	10	theme	reduced	815:821	arg1	performance					823:833	reduced performance	815:833	reduced performance for biofilm removal	815:853	Extracellular polymeric substances in Escherichia coli O157: H7 biofilms were most resistant to photocatalytic oxidation, which led to reduced performance for biofilm removal.
33370693	1	11	theme	environmental	170:182	arg1	pathogens					184:192	inactivating environmental pathogens	157:192	inactivating environmental pathogens	157:192	Photocatalysis holds promise for inactivating environmental pathogens.
33370693	4	12	from	substances	704:713	arg1	biofilms					744:751	Escherichia coli O157: H7 biofilms	718:751	Escherichia coli O157: H7 biofilms	718:751	Extracellular polymeric substances in Escherichia coli O157: H7 biofilms were most resistant to photocatalytic oxidation, which led to reduced performance for biofilm removal.
33370693	2	13	theme	Visible-light-responsive	195:218	arg1	chitosan					276:283	chitosan	276:283	chitosan	276:283	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications.
33370693	2	13	theme	Visible-light-responsive	195:218	arg1	nitride					264:270	carbon-doped graphitic carbon nitride	234:270	carbon-doped graphitic carbon nitride	234:270	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications.
33370693	2	13	theme	Visible-light-responsive	195:218	arg1	composites					220:229	Visible-light-responsive composites	195:229	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability	195:323	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications.
33370693	4	14	theme	photocatalytic	776:789	arg1	oxidation					791:799	photocatalytic oxidation	776:799	photocatalytic oxidation	776:799	Extracellular polymeric substances in Escherichia coli O157: H7 biofilms were most resistant to photocatalytic oxidation, which led to reduced performance for biofilm removal.
33370693	7	15	theme	microbiological	1307:1321	arg1	system					1323:1328	a microbiological system	1305:1328	a microbiological system	1305:1328	Our study provides fundamental insights into the development of new photocatalytic composites, and elucidates the mechanism of how the photocatalyst reacts with a microbiological system.
33370693	3	16	theme	visible	635:641	arg1	irradiation					649:659	visible light irradiation	635:659	visible light irradiation	635:659	The broad-spectrum biofilm inhibition and eradication of the photocatalytic composites against Staphylococcus epidermidis, Pseudomonas aeruginosa PAO1, and Escherichia coli O157: H7 under visible light irradiation were demonstrated.
33370693	0	17	theme	graphitic	15:23	arg1	composites					49:58	Photocatalytic graphitic carbon nitride-chitosan composites	0:58	Photocatalytic graphitic carbon nitride-chitosan composites for pathogenic biofilm	0:81	Photocatalytic graphitic carbon nitride-chitosan composites for pathogenic biofilm control under visible light irradiation.
33370693	6	18	theme	multiple	1116:1123	arg1	species					1135:1141	multiple bacterial species	1116:1141	multiple bacterial species	1116:1141	Moreover, the composites exhibited excellent performance for inhibiting biofilm development in urine, highlighting the promise for inactivating environmental biofilms developed from multiple bacterial species.
33370693	3	19	theme	photocatalytic	508:521	arg1	composites					523:532	the photocatalytic composites	504:532	the photocatalytic composites against Staphylococcus epidermidis	504:567	The broad-spectrum biofilm inhibition and eradication of the photocatalytic composites against Staphylococcus epidermidis, Pseudomonas aeruginosa PAO1, and Escherichia coli O157: H7 under visible light irradiation were demonstrated.
33370693	0	20	theme	Photocatalytic	0:13	arg1	composites					49:58	Photocatalytic graphitic carbon nitride-chitosan composites	0:58	Photocatalytic graphitic carbon nitride-chitosan composites for pathogenic biofilm	0:81	Photocatalytic graphitic carbon nitride-chitosan composites for pathogenic biofilm control under visible light irradiation.
33370693	4	21	theme	biofilm	839:845	arg1	removal					847:853	biofilm removal	839:853	biofilm removal	839:853	Extracellular polymeric substances in Escherichia coli O157: H7 biofilms were most resistant to photocatalytic oxidation, which led to reduced performance for biofilm removal.
33370693	2	22	theme	environmental	387:399	arg1	applications					433:444	environmental, food, biomedical, and building applications	387:444	environmental, food, biomedical, and building applications	387:444	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications.
33370693	3	23	theme	composites	523:532	arg1	eradication					489:499	eradication	489:499	eradication	489:499	The broad-spectrum biofilm inhibition and eradication of the photocatalytic composites against Staphylococcus epidermidis, Pseudomonas aeruginosa PAO1, and Escherichia coli O157: H7 under visible light irradiation were demonstrated.
33370693	3	23	theme	composites	523:532	arg1	inhibition					474:483	The broad-spectrum biofilm inhibition	447:483	The broad-spectrum biofilm inhibition	447:483	The broad-spectrum biofilm inhibition and eradication of the photocatalytic composites against Staphylococcus epidermidis, Pseudomonas aeruginosa PAO1, and Escherichia coli O157: H7 under visible light irradiation were demonstrated.
33370693	0	24	theme	nitride-chitosan	32:47	arg1	composites					49:58	Photocatalytic graphitic carbon nitride-chitosan composites	0:58	Photocatalytic graphitic carbon nitride-chitosan composites for pathogenic biofilm	0:81	Photocatalytic graphitic carbon nitride-chitosan composites for pathogenic biofilm control under visible light irradiation.
33370693	3	25	theme	Pseudomonas	570:580	arg1	PAO1					593:596	Pseudomonas aeruginosa PAO1	570:596	Pseudomonas aeruginosa PAO1	570:596	The broad-spectrum biofilm inhibition and eradication of the photocatalytic composites against Staphylococcus epidermidis, Pseudomonas aeruginosa PAO1, and Escherichia coli O157: H7 under visible light irradiation were demonstrated.
33370693	0	26	theme	carbon	25:30	arg1	composites					49:58	Photocatalytic graphitic carbon nitride-chitosan composites	0:58	Photocatalytic graphitic carbon nitride-chitosan composites for pathogenic biofilm	0:81	Photocatalytic graphitic carbon nitride-chitosan composites for pathogenic biofilm control under visible light irradiation.
33370693	4	27	theme	Extracellular	680:692	arg1	substances					704:713	Extracellular polymeric substances	680:713	Extracellular polymeric substances in Escherichia coli O157: H7 biofilms	680:751	Extracellular polymeric substances in Escherichia coli O157: H7 biofilms were most resistant to photocatalytic oxidation, which led to reduced performance for biofilm removal.
33370693	2	28	theme	carbon	257:262	arg1	nitride					264:270	carbon-doped graphitic carbon nitride	234:270	carbon-doped graphitic carbon nitride	234:270	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications.
33370693	3	29	dep	O157	620:623	arg1	coli					615:618	Escherichia coli O157: H7	603:627	Escherichia coli O157: H7	603:627	The broad-spectrum biofilm inhibition and eradication of the photocatalytic composites against Staphylococcus epidermidis, Pseudomonas aeruginosa PAO1, and Escherichia coli O157: H7 under visible light irradiation were demonstrated.
33370693	3	29	dep	O157	620:623	arg1	H7					626:627	H7	626:627	Escherichia coli O157: H7	603:627	The broad-spectrum biofilm inhibition and eradication of the photocatalytic composites against Staphylococcus epidermidis, Pseudomonas aeruginosa PAO1, and Escherichia coli O157: H7 under visible light irradiation were demonstrated.
33370693	3	30	theme	Escherichia	603:613	arg1	O157					620:623	O157	620:623	Escherichia coli O157: H7	603:627	The broad-spectrum biofilm inhibition and eradication of the photocatalytic composites against Staphylococcus epidermidis, Pseudomonas aeruginosa PAO1, and Escherichia coli O157: H7 under visible light irradiation were demonstrated.
33370693	2	31	theme	graphitic	247:255	arg1	nitride					264:270	carbon-doped graphitic carbon nitride	234:270	carbon-doped graphitic carbon nitride	234:270	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications.
33370693	2	32	with	composites	220:229	arg1	processability					310:323	processability	310:323	processability	310:323	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications.
33370693	2	32	with	composites	220:229	arg1	reactivity					295:304	high reactivity	290:304	high reactivity	290:304	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications.
33370693	2	33	theme	carbon-doped	234:245	arg1	nitride					264:270	carbon-doped graphitic carbon nitride	234:270	carbon-doped graphitic carbon nitride	234:270	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications.
33370693	5	34	theme	biofilm	912:918	arg1	inactivation					920:931	biofilm inactivation	912:931	biofilm inactivation	912:931	1O2 produced by the composites was believed to dominate biofilm inactivation.
33370693	7	35	theme	photocatalytic	1212:1225	arg1	composites					1227:1236	new photocatalytic composites	1208:1236	new photocatalytic composites	1208:1236	Our study provides fundamental insights into the development of new photocatalytic composites, and elucidates the mechanism of how the photocatalyst reacts with a microbiological system.
33370693	6	36	theme	excellent	969:977	arg1	performance					979:989	excellent performance	969:989	excellent performance for inhibiting biofilm development in urine	969:1033	Moreover, the composites exhibited excellent performance for inhibiting biofilm development in urine, highlighting the promise for inactivating environmental biofilms developed from multiple bacterial species.
33370693	0	37	theme	pathogenic	64:73	arg1	biofilm					75:81	pathogenic biofilm	64:81	pathogenic biofilm	64:81	Photocatalytic graphitic carbon nitride-chitosan composites for pathogenic biofilm control under visible light irradiation.
33370693	7	38	theme	composites	1227:1236	arg1	development					1193:1203	the development	1189:1203	the development of new photocatalytic composites	1189:1236	Our study provides fundamental insights into the development of new photocatalytic composites, and elucidates the mechanism of how the photocatalyst reacts with a microbiological system.
33370693	2	39	theme	high	290:293	arg1	reactivity					295:304	high reactivity	290:304	high reactivity	290:304	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications.
33370693	2	40	theme	biomedical	408:417	arg1	applications					433:444	environmental, food, biomedical, and building applications	387:444	environmental, food, biomedical, and building applications	387:444	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications.
33370693	6	41	theme	environmental	1078:1090	arg1	biofilms					1092:1099	inactivating environmental biofilms	1065:1099	inactivating environmental biofilms developed from multiple bacterial species	1065:1141	Moreover, the composites exhibited excellent performance for inhibiting biofilm development in urine, highlighting the promise for inactivating environmental biofilms developed from multiple bacterial species.
33370693	2	42	theme	chitosan	276:283	arg1	chitosan					276:283	chitosan	276:283	chitosan	276:283	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications.
33370693	2	42	theme	chitosan	276:283	arg1	nitride					264:270	carbon-doped graphitic carbon nitride	234:270	carbon-doped graphitic carbon nitride	234:270	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications.
33370693	2	42	theme	chitosan	276:283	arg1	composites					220:229	Visible-light-responsive composites	195:229	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability	195:323	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications.
33370693	1	43	theme	inactivating	157:168	arg1	pathogens					184:192	inactivating environmental pathogens	157:192	inactivating environmental pathogens	157:192	Photocatalysis holds promise for inactivating environmental pathogens.
33370693	4	44	theme	O157	735:738	arg1	biofilms					744:751	Escherichia coli O157: H7 biofilms	718:751	Escherichia coli O157: H7 biofilms	718:751	Extracellular polymeric substances in Escherichia coli O157: H7 biofilms were most resistant to photocatalytic oxidation, which led to reduced performance for biofilm removal.
33370693	7	45	theme	new	1208:1210	arg1	composites					1227:1236	new photocatalytic composites	1208:1236	new photocatalytic composites	1208:1236	Our study provides fundamental insights into the development of new photocatalytic composites, and elucidates the mechanism of how the photocatalyst reacts with a microbiological system.
33370693	4	46	dep	O157	735:738	arg1	H7					741:742	H7	741:742	Escherichia coli O157: H7 biofilms	718:751	Extracellular polymeric substances in Escherichia coli O157: H7 biofilms were most resistant to photocatalytic oxidation, which led to reduced performance for biofilm removal.
33370693	3	47	theme	light	643:647	arg1	irradiation					649:659	visible light irradiation	635:659	visible light irradiation	635:659	The broad-spectrum biofilm inhibition and eradication of the photocatalytic composites against Staphylococcus epidermidis, Pseudomonas aeruginosa PAO1, and Escherichia coli O157: H7 under visible light irradiation were demonstrated.
33370693	2	48	theme	food	402:405	arg1	applications					433:444	environmental, food, biomedical, and building applications	387:444	environmental, food, biomedical, and building applications	387:444	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications.
33370693	3	49	theme	PAO1	593:596	arg1	eradication					489:499	eradication	489:499	eradication	489:499	The broad-spectrum biofilm inhibition and eradication of the photocatalytic composites against Staphylococcus epidermidis, Pseudomonas aeruginosa PAO1, and Escherichia coli O157: H7 under visible light irradiation were demonstrated.
33370693	3	49	theme	PAO1	593:596	arg1	inhibition					474:483	The broad-spectrum biofilm inhibition	447:483	The broad-spectrum biofilm inhibition	447:483	The broad-spectrum biofilm inhibition and eradication of the photocatalytic composites against Staphylococcus epidermidis, Pseudomonas aeruginosa PAO1, and Escherichia coli O157: H7 under visible light irradiation were demonstrated.
33370693	6	50	theme	biofilm	1006:1012	arg1	development					1014:1024	inhibiting biofilm development	995:1024	inhibiting biofilm development	995:1024	Moreover, the composites exhibited excellent performance for inhibiting biofilm development in urine, highlighting the promise for inactivating environmental biofilms developed from multiple bacterial species.
33370693	2	51	theme	nitride	264:270	arg1	chitosan					276:283	chitosan	276:283	chitosan	276:283	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications.
33370693	2	51	theme	nitride	264:270	arg1	nitride					264:270	carbon-doped graphitic carbon nitride	234:270	carbon-doped graphitic carbon nitride	234:270	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications.
33370693	2	51	theme	nitride	264:270	arg1	composites					220:229	Visible-light-responsive composites	195:229	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability	195:323	Visible-light-responsive composites of carbon-doped graphitic carbon nitride and chitosan with high reactivity and processability were fabricated, and they can control pathogenic biofilms for environmental, food, biomedical, and building applications.
33370693	3	52	dep	Pseudomonas	570:580	arg1	aeruginosa					582:591	aeruginosa	582:591	aeruginosa	582:591	The broad-spectrum biofilm inhibition and eradication of the photocatalytic composites against Staphylococcus epidermidis, Pseudomonas aeruginosa PAO1, and Escherichia coli O157: H7 under visible light irradiation were demonstrated.
33370693	7	53	theme	fundamental	1163:1173	arg1	insights					1175:1182	fundamental insights	1163:1182	fundamental insights into the development of new photocatalytic composites	1163:1236	Our study provides fundamental insights into the development of new photocatalytic composites, and elucidates the mechanism of how the photocatalyst reacts with a microbiological system.
33370693	6	54	from	performance	979:989	arg1	urine					1029:1033	urine	1029:1033	urine	1029:1033	Moreover, the composites exhibited excellent performance for inhibiting biofilm development in urine, highlighting the promise for inactivating environmental biofilms developed from multiple bacterial species.
33370693	6	55	theme	inactivating	1065:1076	arg1	biofilms					1092:1099	inactivating environmental biofilms	1065:1099	inactivating environmental biofilms developed from multiple bacterial species	1065:1141	Moreover, the composites exhibited excellent performance for inhibiting biofilm development in urine, highlighting the promise for inactivating environmental biofilms developed from multiple bacterial species.
33370693	3	56	theme	broad-spectrum	451:464	arg1	inhibition					474:483	The broad-spectrum biofilm inhibition	447:483	The broad-spectrum biofilm inhibition	447:483	The broad-spectrum biofilm inhibition and eradication of the photocatalytic composites against Staphylococcus epidermidis, Pseudomonas aeruginosa PAO1, and Escherichia coli O157: H7 under visible light irradiation were demonstrated.
33370693	3	57	theme	biofilm	466:472	arg1	inhibition					474:483	The broad-spectrum biofilm inhibition	447:483	The broad-spectrum biofilm inhibition	447:483	The broad-spectrum biofilm inhibition and eradication of the photocatalytic composites against Staphylococcus epidermidis, Pseudomonas aeruginosa PAO1, and Escherichia coli O157: H7 under visible light irradiation were demonstrated.
32473217	0	0	theme	peroxide	88:95	arg1	treatment					97:105	hydrogen peroxide treatment	79:105	hydrogen peroxide treatment	79:105	Degradation of Tremella fuciformis polysaccharide by a combined ultrasound and hydrogen peroxide treatment: Process parameters, structural characteristics, and antioxidant activities.
32473217	5	1	theme	microscopy	1095:1104	arg1	results					1121:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results	933:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results	933:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	5	2	theme	atomic	1082:1087	arg1	microscopy					1095:1104	atomic force microscopy	1082:1104	atomic force microscopy	1082:1104	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	3	3	theme	degradation	496:506	arg1	higher					544:549	higher	544:549	higher	544:549	The results showed that the degradation effect of US/H2O2 was significantly higher than that of US or H2O2 alone, and the degradation of TFP was dependent on the duration of its exposure to US, the ultrasonic amplitude, and the H2O2 and TFP concentrations.
32473217	3	3	theme	degradation	496:506	arg1	effect					508:513	the degradation effect	492:513	the degradation effect of US/H2O2	492:524	The results showed that the degradation effect of US/H2O2 was significantly higher than that of US or H2O2 alone, and the degradation of TFP was dependent on the duration of its exposure to US, the ultrasonic amplitude, and the H2O2 and TFP concentrations.
32473217	6	4	theme	antioxidant	1443:1453	arg1	activity					1455:1462	a higher antioxidant activity	1434:1462	a higher antioxidant activity than the original TFP	1434:1484	Additionally, the degraded TFPs with low molecular weight exhibited a higher antioxidant activity than the original TFP.
32473217	7	5	theme	simple	1542:1547	arg1	method					1563:1568	a simple and effective method	1540:1568	a simple and effective method to prepare a TFP of low molecular weight and high bioactivity	1540:1630	These findings suggest that the US/H2O2 treatment is a simple and effective method to prepare a TFP of low molecular weight and high bioactivity.
32473217	7	5	theme	simple	1542:1547	arg1	treatment					1527:1535	the US/H2O2 treatment	1515:1535	the US/H2O2 treatment	1515:1535	These findings suggest that the US/H2O2 treatment is a simple and effective method to prepare a TFP of low molecular weight and high bioactivity.
32473217	0	6	theme	hydrogen	79:86	arg1	peroxide					88:95	hydrogen peroxide	79:95	hydrogen peroxide treatment	79:105	Degradation of Tremella fuciformis polysaccharide by a combined ultrasound and hydrogen peroxide treatment: Process parameters, structural characteristics, and antioxidant activities.
32473217	2	7	theme	degradation	340:350	arg1	parameters					326:335	The main process parameters	309:335	The main process parameters of degradation	309:350	The main process parameters of degradation were evaluated and the structural changes and antioxidant activities of TFP before and after US/H2O2 were analyzed.
32473217	6	8	theme	higher	1436:1441	arg1	activity					1455:1462	a higher antioxidant activity	1434:1462	a higher antioxidant activity than the original TFP	1434:1484	Additionally, the degraded TFPs with low molecular weight exhibited a higher antioxidant activity than the original TFP.
32473217	6	9	theme	low	1403:1405	arg1	weight					1417:1422	low molecular weight	1403:1422	low molecular weight	1403:1422	Additionally, the degraded TFPs with low molecular weight exhibited a higher antioxidant activity than the original TFP.
32473217	5	10	theme	High	933:936	arg1	chromatography					957:970	High performance liquid chromatography	933:970	High performance liquid chromatography	933:970	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	1	11	theme	Tremella	254:261	arg1	TFP					290:292	TFP	290:292	TFP	290:292	The degradation effect of ultrasound (US)/hydrogen peroxide (H2O2) on Tremella fuciformis polysaccharide (TFP) were studied.
32473217	1	11	theme	Tremella	254:261	arg1	polysaccharide					274:287	Tremella fuciformis polysaccharide	254:287	Tremella fuciformis polysaccharide (TFP)	254:293	The degradation effect of ultrasound (US)/hydrogen peroxide (H2O2) on Tremella fuciformis polysaccharide (TFP) were studied.
32473217	5	12	theme	electron	1061:1068	arg1	microscopy					1070:1079	scanning electron microscopy	1052:1079	scanning electron microscopy	1052:1079	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	7	13	theme	bioactivity	1620:1630	arg1	TFP					1583:1585	a TFP	1581:1585	a TFP of low molecular weight and high bioactivity	1581:1630	These findings suggest that the US/H2O2 treatment is a simple and effective method to prepare a TFP of low molecular weight and high bioactivity.
32473217	1	14	theme	fuciformis	263:272	arg1	TFP					290:292	TFP	290:292	TFP	290:292	The degradation effect of ultrasound (US)/hydrogen peroxide (H2O2) on Tremella fuciformis polysaccharide (TFP) were studied.
32473217	1	14	theme	fuciformis	263:272	arg1	polysaccharide					274:287	Tremella fuciformis polysaccharide	254:287	Tremella fuciformis polysaccharide (TFP)	254:293	The degradation effect of ultrasound (US)/hydrogen peroxide (H2O2) on Tremella fuciformis polysaccharide (TFP) were studied.
32473217	5	15	theme	microscopy	1070:1079	arg1	results					1121:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results	933:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results	933:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	5	16	theme	polysaccharide	1179:1192	arg1	chains					1194:1199	the polysaccharide chains	1175:1199	the polysaccharide chains	1175:1199	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	0	17	theme	Process	108:114	arg1	parameters					116:125	Process parameters	108:125	Process parameters	108:125	Degradation of Tremella fuciformis polysaccharide by a combined ultrasound and hydrogen peroxide treatment: Process parameters, structural characteristics, and antioxidant activities.
32473217	3	18	theme	ultrasonic	666:675	arg1	amplitude					677:685	the ultrasonic amplitude	662:685	the ultrasonic amplitude	662:685	The results showed that the degradation effect of US/H2O2 was significantly higher than that of US or H2O2 alone, and the degradation of TFP was dependent on the duration of its exposure to US, the ultrasonic amplitude, and the H2O2 and TFP concentrations.
32473217	4	19	theme	molecular	884:892	arg1	weight					894:899	its molecular weight	880:899	its molecular weight	880:899	US/H2O2 reduced the molecular weight (from 8.14 × 105 Da to 1.27 × 104 Da) and particle size (from 710 nm to 182 nm) of the TFP within 50 min and narrowed its molecular weight and particle size distribution.
32473217	5	20	theme	primary	1309:1315	arg1	structure					1317:1325	primary structure	1309:1325	primary structure	1309:1325	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	1	21	theme	degradation	188:198	arg1	effect					200:205	The degradation effect	184:205	The degradation effect of ultrasound (US)/hydrogen peroxide (H2O2) on Tremella fuciformis polysaccharide (TFP)	184:293	The degradation effect of ultrasound (US)/hydrogen peroxide (H2O2) on Tremella fuciformis polysaccharide (TFP) were studied.
32473217	4	22	theme	size	914:917	arg1	distribution					919:930	particle size distribution	905:930	particle size distribution	905:930	US/H2O2 reduced the molecular weight (from 8.14 × 105 Da to 1.27 × 104 Da) and particle size (from 710 nm to 182 nm) of the TFP within 50 min and narrowed its molecular weight and particle size distribution.
32473217	5	23	theme	performance	938:948	arg1	chromatography					957:970	High performance liquid chromatography	933:970	High performance liquid chromatography	933:970	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	2	24	theme	structural	375:384	arg1	changes					386:392	the structural changes	371:392	the structural changes	371:392	The main process parameters of degradation were evaluated and the structural changes and antioxidant activities of TFP before and after US/H2O2 were analyzed.
32473217	4	25	theme	particle	804:811	arg1	size					813:816	particle size	804:816	particle size (from 710 nm to 182 nm)	804:840	US/H2O2 reduced the molecular weight (from 8.14 × 105 Da to 1.27 × 104 Da) and particle size (from 710 nm to 182 nm) of the TFP within 50 min and narrowed its molecular weight and particle size distribution.
32473217	0	26	theme	fuciformis	24:33	arg1	polysaccharide					35:48	Tremella fuciformis polysaccharide	15:48	Tremella fuciformis polysaccharide	15:48	Degradation of Tremella fuciformis polysaccharide by a combined ultrasound and hydrogen peroxide treatment: Process parameters, structural characteristics, and antioxidant activities.
32473217	3	27	theme	TFP	705:707	arg1	concentrations					709:722	the H2O2 and TFP concentrations	692:722	concentrations	709:722	The results showed that the degradation effect of US/H2O2 was significantly higher than that of US or H2O2 alone, and the degradation of TFP was dependent on the duration of its exposure to US, the ultrasonic amplitude, and the H2O2 and TFP concentrations.
32473217	0	28	theme	structural	128:137	arg1	characteristics					139:153	structural characteristics	128:153	structural characteristics	128:153	Degradation of Tremella fuciformis polysaccharide by a combined ultrasound and hydrogen peroxide treatment: Process parameters, structural characteristics, and antioxidant activities.
32473217	6	29	with	TFPs	1393:1396	arg1	weight					1417:1422	low molecular weight	1403:1422	low molecular weight	1403:1422	Additionally, the degraded TFPs with low molecular weight exhibited a higher antioxidant activity than the original TFP.
32473217	5	30	theme	TFP	1274:1276	arg1	molecules					1278:1286	the TFP molecules	1270:1286	the TFP molecules	1270:1286	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	2	31	theme	TFP	424:426	arg1	changes					386:392	the structural changes	371:392	the structural changes	371:392	The main process parameters of degradation were evaluated and the structural changes and antioxidant activities of TFP before and after US/H2O2 were analyzed.
32473217	2	31	theme	TFP	424:426	arg1	activities					410:419	antioxidant activities	398:419	antioxidant activities of TFP	398:426	The main process parameters of degradation were evaluated and the structural changes and antioxidant activities of TFP before and after US/H2O2 were analyzed.
32473217	0	32	theme	Tremella	15:22	arg1	polysaccharide					35:48	Tremella fuciformis polysaccharide	15:48	Tremella fuciformis polysaccharide	15:48	Degradation of Tremella fuciformis polysaccharide by a combined ultrasound and hydrogen peroxide treatment: Process parameters, structural characteristics, and antioxidant activities.
32473217	7	33	theme	molecular	1594:1602	arg1	weight					1604:1609	low molecular weight	1590:1609	low molecular weight	1590:1609	These findings suggest that the US/H2O2 treatment is a simple and effective method to prepare a TFP of low molecular weight and high bioactivity.
32473217	4	34	theme	TFP	849:851	arg1	weight					755:760	the molecular weight	741:760	the molecular weight (from 8.14 × 105 Da to 1.27 × 104 Da)	741:798	US/H2O2 reduced the molecular weight (from 8.14 × 105 Da to 1.27 × 104 Da) and particle size (from 710 nm to 182 nm) of the TFP within 50 min and narrowed its molecular weight and particle size distribution.
32473217	4	34	theme	TFP	849:851	arg1	size					813:816	particle size	804:816	particle size (from 710 nm to 182 nm)	804:840	US/H2O2 reduced the molecular weight (from 8.14 × 105 Da to 1.27 × 104 Da) and particle size (from 710 nm to 182 nm) of the TFP within 50 min and narrowed its molecular weight and particle size distribution.
32473217	5	35	theme	magnetic	1032:1039	arg1	resonance					1041:1049	Carbon-13 nuclear magnetic resonance	1014:1049	Carbon-13 nuclear magnetic resonance	1014:1049	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	4	36	dep	1.27 × 104 Da	785:797	arg1	8.14 × 105 Da					768:780	8.14 × 105 Da	768:780	8.14 × 105 Da	768:780	US/H2O2 reduced the molecular weight (from 8.14 × 105 Da to 1.27 × 104 Da) and particle size (from 710 nm to 182 nm) of the TFP within 50 min and narrowed its molecular weight and particle size distribution.
32473217	5	37	theme	molecules	1278:1286	arg1	flexibility					1255:1265	the conformation flexibility	1238:1265	the conformation flexibility of the TFP molecules	1238:1286	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	7	38	theme	weight	1604:1609	arg1	TFP					1583:1585	a TFP	1581:1585	a TFP of low molecular weight and high bioactivity	1581:1630	These findings suggest that the US/H2O2 treatment is a simple and effective method to prepare a TFP of low molecular weight and high bioactivity.
32473217	5	39	theme	monosaccharide	1331:1344	arg1	composition					1346:1356	monosaccharide composition	1331:1356	monosaccharide composition	1331:1356	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	3	40	theme	H2O2	696:699	arg1	concentrations					709:722	the H2O2 and TFP concentrations	692:722	concentrations	709:722	The results showed that the degradation effect of US/H2O2 was significantly higher than that of US or H2O2 alone, and the degradation of TFP was dependent on the duration of its exposure to US, the ultrasonic amplitude, and the H2O2 and TFP concentrations.
32473217	7	41	theme	US/H2O2	1519:1525	arg1	method					1563:1568	a simple and effective method	1540:1568	a simple and effective method to prepare a TFP of low molecular weight and high bioactivity	1540:1630	These findings suggest that the US/H2O2 treatment is a simple and effective method to prepare a TFP of low molecular weight and high bioactivity.
32473217	7	41	theme	US/H2O2	1519:1525	arg1	treatment					1527:1535	the US/H2O2 treatment	1515:1535	the US/H2O2 treatment	1515:1535	These findings suggest that the US/H2O2 treatment is a simple and effective method to prepare a TFP of low molecular weight and high bioactivity.
32473217	5	42	theme	resonance	1041:1049	arg1	results					1121:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results	933:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results	933:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	5	43	theme	chromatography	957:970	arg1	results					1121:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results	933:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results	933:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	0	44	theme	polysaccharide	35:48	arg1	Degradation					0:10	Degradation	0:10	Degradation of Tremella fuciformis polysaccharide by a combined ultrasound and hydrogen peroxide treatment: Process parameters, structural characteristics, and antioxidant activities.	0:182	Degradation of Tremella fuciformis polysaccharide by a combined ultrasound and hydrogen peroxide treatment: Process parameters, structural characteristics, and antioxidant activities.
32473217	5	45	theme	Congo	1111:1115	arg1	results					1121:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results	933:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results	933:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	6	46	theme	molecular	1407:1415	arg1	weight					1417:1422	low molecular weight	1403:1422	low molecular weight	1403:1422	Additionally, the degraded TFPs with low molecular weight exhibited a higher antioxidant activity than the original TFP.
32473217	5	47	theme	TFP	1361:1363	arg1	composition					1346:1356	monosaccharide composition	1331:1356	monosaccharide composition	1331:1356	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	5	47	theme	TFP	1361:1363	arg1	structure					1317:1325	primary structure	1309:1325	primary structure	1309:1325	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	2	48	theme	antioxidant	398:408	arg1	activities					410:419	antioxidant activities	398:419	antioxidant activities of TFP	398:426	The main process parameters of degradation were evaluated and the structural changes and antioxidant activities of TFP before and after US/H2O2 were analyzed.
32473217	0	49	theme	combined	55:62	arg1	ultrasound					64:73	a combined ultrasound	53:73	a combined ultrasound	53:73	Degradation of Tremella fuciformis polysaccharide by a combined ultrasound and hydrogen peroxide treatment: Process parameters, structural characteristics, and antioxidant activities.
32473217	5	50	theme	red	1117:1119	arg1	results					1121:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results	933:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results	933:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	0	51	theme	antioxidant	160:170	arg1	activities					172:181	antioxidant activities	160:181	antioxidant activities	160:181	Degradation of Tremella fuciformis polysaccharide by a combined ultrasound and hydrogen peroxide treatment: Process parameters, structural characteristics, and antioxidant activities.
32473217	5	52	theme	scanning	1052:1059	arg1	microscopy					1070:1079	scanning electron microscopy	1052:1079	scanning electron microscopy	1052:1079	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	5	53	theme	Fourier-transform	973:989	arg1	spectroscopy					1000:1011	Fourier-transform infrared spectroscopy	973:1011	Fourier-transform infrared spectroscopy	973:1011	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	5	54	dep	structure	1317:1325	arg1	the					1305:1307	the	1305:1307	the	1305:1307	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	4	55	dep	182 nm	834:839	arg1	710 nm					824:829	710 nm	824:829	710 nm	824:829	US/H2O2 reduced the molecular weight (from 8.14 × 105 Da to 1.27 × 104 Da) and particle size (from 710 nm to 182 nm) of the TFP within 50 min and narrowed its molecular weight and particle size distribution.
32473217	5	56	theme	spectroscopy	1000:1011	arg1	results					1121:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results	933:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results	933:1127	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	5	57	theme	infrared	991:998	arg1	spectroscopy					1000:1011	Fourier-transform infrared spectroscopy	973:1011	Fourier-transform infrared spectroscopy	973:1011	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	5	58	theme	liquid	950:955	arg1	chromatography					957:970	High performance liquid chromatography	933:970	High performance liquid chromatography	933:970	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	3	59	theme	exposure	646:653	arg1	amplitude					677:685	the ultrasonic amplitude	662:685	the ultrasonic amplitude	662:685	The results showed that the degradation effect of US/H2O2 was significantly higher than that of US or H2O2 alone, and the degradation of TFP was dependent on the duration of its exposure to US, the ultrasonic amplitude, and the H2O2 and TFP concentrations.
32473217	3	59	theme	exposure	646:653	arg1	concentrations					709:722	the H2O2 and TFP concentrations	692:722	concentrations	709:722	The results showed that the degradation effect of US/H2O2 was significantly higher than that of US or H2O2 alone, and the degradation of TFP was dependent on the duration of its exposure to US, the ultrasonic amplitude, and the H2O2 and TFP concentrations.
32473217	3	59	theme	exposure	646:653	arg1	duration					630:637	the duration	626:637	the duration of its exposure to US	626:659	The results showed that the degradation effect of US/H2O2 was significantly higher than that of US or H2O2 alone, and the degradation of TFP was dependent on the duration of its exposure to US, the ultrasonic amplitude, and the H2O2 and TFP concentrations.
32473217	4	60	theme	molecular	745:753	arg1	weight					755:760	the molecular weight	741:760	the molecular weight (from 8.14 × 105 Da to 1.27 × 104 Da)	741:798	US/H2O2 reduced the molecular weight (from 8.14 × 105 Da to 1.27 × 104 Da) and particle size (from 710 nm to 182 nm) of the TFP within 50 min and narrowed its molecular weight and particle size distribution.
32473217	2	61	theme	process	318:324	arg1	parameters					326:335	The main process parameters	309:335	The main process parameters of degradation	309:350	The main process parameters of degradation were evaluated and the structural changes and antioxidant activities of TFP before and after US/H2O2 were analyzed.
32473217	5	62	theme	conformation	1242:1253	arg1	flexibility					1255:1265	the conformation flexibility	1238:1265	the conformation flexibility of the TFP molecules	1238:1286	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	1	63	theme	/hydrogen	225:233	arg1	H2O2					245:248	H2O2	245:248	H2O2	245:248	The degradation effect of ultrasound (US)/hydrogen peroxide (H2O2) on Tremella fuciformis polysaccharide (TFP) were studied.
32473217	1	63	theme	/hydrogen	225:233	arg1	peroxide					235:242	ultrasound (US)/hydrogen peroxide	210:242	ultrasound (US)/hydrogen peroxide (H2O2)	210:249	The degradation effect of ultrasound (US)/hydrogen peroxide (H2O2) on Tremella fuciformis polysaccharide (TFP) were studied.
32473217	5	64	theme	Carbon-13	1014:1022	arg1	resonance					1041:1049	Carbon-13 nuclear magnetic resonance	1014:1049	Carbon-13 nuclear magnetic resonance	1014:1049	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	3	65	theme	TFP	605:607	arg1	degradation					590:600	the degradation	586:600	the degradation of TFP	586:607	The results showed that the degradation effect of US/H2O2 was significantly higher than that of US or H2O2 alone, and the degradation of TFP was dependent on the duration of its exposure to US, the ultrasonic amplitude, and the H2O2 and TFP concentrations.
32473217	3	65	theme	TFP	605:607	arg1	dependent					613:621	dependent	613:621	dependent	613:621	The results showed that the degradation effect of US/H2O2 was significantly higher than that of US or H2O2 alone, and the degradation of TFP was dependent on the duration of its exposure to US, the ultrasonic amplitude, and the H2O2 and TFP concentrations.
32473217	1	66	from	effect	200:205	arg1	TFP					290:292	TFP	290:292	TFP	290:292	The degradation effect of ultrasound (US)/hydrogen peroxide (H2O2) on Tremella fuciformis polysaccharide (TFP) were studied.
32473217	1	66	from	effect	200:205	arg1	polysaccharide					274:287	Tremella fuciformis polysaccharide	254:287	Tremella fuciformis polysaccharide (TFP)	254:293	The degradation effect of ultrasound (US)/hydrogen peroxide (H2O2) on Tremella fuciformis polysaccharide (TFP) were studied.
32473217	2	67	theme	main	313:316	arg1	parameters					326:335	The main process parameters	309:335	The main process parameters of degradation	309:350	The main process parameters of degradation were evaluated and the structural changes and antioxidant activities of TFP before and after US/H2O2 were analyzed.
32473217	1	68	theme	peroxide	235:242	arg1	effect					200:205	The degradation effect	184:205	The degradation effect of ultrasound (US)/hydrogen peroxide (H2O2) on Tremella fuciformis polysaccharide (TFP)	184:293	The degradation effect of ultrasound (US)/hydrogen peroxide (H2O2) on Tremella fuciformis polysaccharide (TFP) were studied.
32473217	4	69	theme	particle	905:912	arg1	distribution					919:930	particle size distribution	905:930	particle size distribution	905:930	US/H2O2 reduced the molecular weight (from 8.14 × 105 Da to 1.27 × 104 Da) and particle size (from 710 nm to 182 nm) of the TFP within 50 min and narrowed its molecular weight and particle size distribution.
32473217	5	70	theme	nuclear	1024:1030	arg1	resonance					1041:1049	Carbon-13 nuclear magnetic resonance	1014:1049	Carbon-13 nuclear magnetic resonance	1014:1049	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	1	71	dep	ultrasound	210:219	arg1	US					222:223	US	222:223	US	222:223	The degradation effect of ultrasound (US)/hydrogen peroxide (H2O2) on Tremella fuciformis polysaccharide (TFP) were studied.
32473217	1	72	theme	ultrasound	210:219	arg1	H2O2					245:248	H2O2	245:248	H2O2	245:248	The degradation effect of ultrasound (US)/hydrogen peroxide (H2O2) on Tremella fuciformis polysaccharide (TFP) were studied.
32473217	1	72	theme	ultrasound	210:219	arg1	peroxide					235:242	ultrasound (US)/hydrogen peroxide	210:242	ultrasound (US)/hydrogen peroxide (H2O2)	210:249	The degradation effect of ultrasound (US)/hydrogen peroxide (H2O2) on Tremella fuciformis polysaccharide (TFP) were studied.
32473217	6	73	theme	original	1473:1480	arg1	TFP					1482:1484	the original TFP	1469:1484	the original TFP	1469:1484	Additionally, the degraded TFPs with low molecular weight exhibited a higher antioxidant activity than the original TFP.
32473217	7	74	theme	effective	1553:1561	arg1	method					1563:1568	a simple and effective method	1540:1568	a simple and effective method to prepare a TFP of low molecular weight and high bioactivity	1540:1630	These findings suggest that the US/H2O2 treatment is a simple and effective method to prepare a TFP of low molecular weight and high bioactivity.
32473217	7	74	theme	effective	1553:1561	arg1	treatment					1527:1535	the US/H2O2 treatment	1515:1535	the US/H2O2 treatment	1515:1535	These findings suggest that the US/H2O2 treatment is a simple and effective method to prepare a TFP of low molecular weight and high bioactivity.
32473217	6	75	theme	degraded	1384:1391	arg1	TFPs					1393:1396	the degraded TFPs	1380:1396	the degraded TFPs with low molecular weight	1380:1422	Additionally, the degraded TFPs with low molecular weight exhibited a higher antioxidant activity than the original TFP.
32473217	5	76	theme	force	1089:1093	arg1	microscopy					1095:1104	atomic force microscopy	1082:1104	atomic force microscopy	1082:1104	High performance liquid chromatography, Fourier-transform infrared spectroscopy, Carbon-13 nuclear magnetic resonance, scanning electron microscopy, atomic force microscopy, and Congo red results indicated that the treatment could break down the polysaccharide chains, hinder the aggregation, and improve the conformation flexibility of the TFP molecules without changing the primary structure and monosaccharide composition of TFP.
32473217	0	77	dep	Degradation	0:10	arg1	characteristics					139:153	structural characteristics	128:153	structural characteristics	128:153	Degradation of Tremella fuciformis polysaccharide by a combined ultrasound and hydrogen peroxide treatment: Process parameters, structural characteristics, and antioxidant activities.
32473217	0	77	dep	Degradation	0:10	arg1	activities					172:181	antioxidant activities	160:181	antioxidant activities	160:181	Degradation of Tremella fuciformis polysaccharide by a combined ultrasound and hydrogen peroxide treatment: Process parameters, structural characteristics, and antioxidant activities.
32473217	0	77	dep	Degradation	0:10	arg1	parameters					116:125	Process parameters	108:125	Process parameters	108:125	Degradation of Tremella fuciformis polysaccharide by a combined ultrasound and hydrogen peroxide treatment: Process parameters, structural characteristics, and antioxidant activities.
32473217	7	78	theme	high	1615:1618	arg1	bioactivity					1620:1630	high bioactivity	1615:1630	high bioactivity	1615:1630	These findings suggest that the US/H2O2 treatment is a simple and effective method to prepare a TFP of low molecular weight and high bioactivity.
32473217	3	79	theme	US/H2O2	518:524	arg1	higher					544:549	higher	544:549	higher	544:549	The results showed that the degradation effect of US/H2O2 was significantly higher than that of US or H2O2 alone, and the degradation of TFP was dependent on the duration of its exposure to US, the ultrasonic amplitude, and the H2O2 and TFP concentrations.
32473217	3	79	theme	US/H2O2	518:524	arg1	effect					508:513	the degradation effect	492:513	the degradation effect of US/H2O2	492:524	The results showed that the degradation effect of US/H2O2 was significantly higher than that of US or H2O2 alone, and the degradation of TFP was dependent on the duration of its exposure to US, the ultrasonic amplitude, and the H2O2 and TFP concentrations.
32473217	7	80	theme	low	1590:1592	arg1	weight					1604:1609	low molecular weight	1590:1609	low molecular weight	1590:1609	These findings suggest that the US/H2O2 treatment is a simple and effective method to prepare a TFP of low molecular weight and high bioactivity.
34637934	9	0	from	applications	1992:2003	arg1	field					2110:2114	the biomedical field	2095:2114	the biomedical field	2095:2114	Therefore, this multifunctional system based on ZIF-8 nanocarriers offered a potential nanoplatform for tumor-targeting theranostics, thus broadening the synthesis and applications of ZIF-8 composite nanoparticles for NIR fluorescence imaging and photothermal therapy in the biomedical field.
34637934	11	1	theme	new	2305:2307	arg1	approach					2344:2351	a new synthesis/encapsulation two-in-one approach	2303:2351	a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures	2303:2446	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	9	2	theme	NIR	2042:2044	arg1	imaging					2059:2065	NIR fluorescence imaging	2042:2065	NIR fluorescence imaging	2042:2065	Therefore, this multifunctional system based on ZIF-8 nanocarriers offered a potential nanoplatform for tumor-targeting theranostics, thus broadening the synthesis and applications of ZIF-8 composite nanoparticles for NIR fluorescence imaging and photothermal therapy in the biomedical field.
34637934	8	3	theme	free	1737:1740	arg1	ICG					1742:1744	free ICG	1737:1744	free ICG	1737:1744	Additionally, the results of in vivo infrared imaging and photothermal therapy of Cy5.5&ICG@ZIF-8-Dex showed enhanced therapeutic efficiency in comparison with free ICG, further confirming its tumor-targeting capability and photothermal capacity.
34637934	9	4	from	synthesis	1978:1986	arg1	field					2110:2114	the biomedical field	2095:2114	the biomedical field	2095:2114	Therefore, this multifunctional system based on ZIF-8 nanocarriers offered a potential nanoplatform for tumor-targeting theranostics, thus broadening the synthesis and applications of ZIF-8 composite nanoparticles for NIR fluorescence imaging and photothermal therapy in the biomedical field.
34637934	6	5	theme	A549-bearing	1268:1279	arg1	mice					1281:1284	A549-bearing mice	1268:1284	A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex	1268:1318	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex demonstrated sufficient accumulations of Cy5.5 at tumor sites due to the enhanced permeability and retention effect.
34637934	11	6	theme	functional	2569:2578	arg1	molecules					2580:2588	hydrophobic cyanine-5.5 (Cy5.5) functional molecules	2537:2588	hydrophobic cyanine-5.5 (Cy5.5) functional molecules	2537:2588	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	13	7	theme	hydrophobic	2895:2905	arg1	molecules					2907:2915	hydrophilic and hydrophobic molecules	2879:2915	hydrophilic and hydrophobic molecules	2879:2915	Based on their enhanced convenience and efficacy to simultaneously encapsulate hydrophilic and hydrophobic molecules, the multifunctional nanocarriers that were prepared in the DMSO/H2O mixed solvents provide a potential nanoplatform toward fluorescence imaging and photothermal therapy for tumor theranostics.
34637934	6	8	theme	tumor	1370:1374	arg1	sites					1376:1380	tumor sites	1370:1380	tumor sites	1370:1380	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex demonstrated sufficient accumulations of Cy5.5 at tumor sites due to the enhanced permeability and retention effect.
34637934	1	9	theme	tumor	296:300	arg1	theranostics					302:313	tumor theranostics	296:313	tumor theranostics in recent decades	296:331	Impressive developments have been achieved with the use of zeolitic imidazolate framework-8 (ZIF-8) as nanocarriers for tumor theranostics in recent decades by incorporating imaging agents and therapeutic drugs within ZIF-8.
34637934	7	10	theme	free	1565:1568	arg1	Cy5.5					1570:1574	free Cy5.5	1565:1574	free Cy5.5	1565:1574	Most impressively, the fluorescent intensity of Cy5.5&ICG@ZIF-8-Dex at tumor site was approximately 40-fold higher than that of free Cy5.5.
34637934	12	11	theme	imaging	2743:2749	arg1	capability					2751:2760	a remarkable fluorescence imaging capability	2717:2760	a remarkable fluorescence imaging capability	2717:2760	The results showed that the co-loading of Cy5.5 and ICG within the ZIF-8 nanoparticles endowed them with a remarkable fluorescence imaging capability and photothermal conversion capacity.
34637934	9	12	theme	photothermal	2071:2082	arg1	therapy					2084:2090	photothermal therapy	2071:2090	photothermal therapy	2071:2090	Therefore, this multifunctional system based on ZIF-8 nanocarriers offered a potential nanoplatform for tumor-targeting theranostics, thus broadening the synthesis and applications of ZIF-8 composite nanoparticles for NIR fluorescence imaging and photothermal therapy in the biomedical field.
34637934	6	13	theme	Cy5.5	1361:1365	arg1	accumulations					1344:1356	sufficient accumulations	1333:1356	sufficient accumulations of Cy5.5 at tumor sites	1333:1380	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex demonstrated sufficient accumulations of Cy5.5 at tumor sites due to the enhanced permeability and retention effect.
34637934	11	14	theme	ZIF-8-Dex	2385:2393	arg1	nanoparticles					2405:2417	uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles	2367:2417	uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures	2367:2446	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	11	15	theme	two-in-one	2333:2342	arg1	approach					2344:2351	a new synthesis/encapsulation two-in-one approach	2303:2351	a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures	2303:2446	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	12	16	theme	ICG	2664:2666	arg1	co-loading					2640:2649	the co-loading	2636:2649	the co-loading of Cy5.5 and ICG within the ZIF-8	2636:2683	The results showed that the co-loading of Cy5.5 and ICG within the ZIF-8 nanoparticles endowed them with a remarkable fluorescence imaging capability and photothermal conversion capacity.
34637934	7	17	theme	tumor	1508:1512	arg1	site					1514:1517	tumor site	1508:1517	tumor site	1508:1517	Most impressively, the fluorescent intensity of Cy5.5&ICG@ZIF-8-Dex at tumor site was approximately 40-fold higher than that of free Cy5.5.
34637934	11	18	theme	hydrophilic	2497:2507	arg1	ICG					2528:2530	ICG	2528:2530	ICG	2528:2530	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	11	18	theme	hydrophilic	2497:2507	arg1	green					2521:2525	hydrophilic indocyanine green	2497:2525	hydrophilic indocyanine green (ICG)	2497:2531	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	3	19	theme	green	852:856	arg1	encapsulation					811:823	the simultaneous encapsulation	794:823	the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5)	794:898	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	9	20	dep	synthesis	1978:1986	arg1	the					1974:1976	the	1974:1976	the	1974:1976	Therefore, this multifunctional system based on ZIF-8 nanocarriers offered a potential nanoplatform for tumor-targeting theranostics, thus broadening the synthesis and applications of ZIF-8 composite nanoparticles for NIR fluorescence imaging and photothermal therapy in the biomedical field.
34637934	7	21	theme	ZIF-8-Dex	1495:1503	arg1	intensity					1472:1480	the fluorescent intensity	1456:1480	the fluorescent intensity of Cy5.5&ICG@ZIF-8-Dex at tumor site	1456:1517	Most impressively, the fluorescent intensity of Cy5.5&ICG@ZIF-8-Dex at tumor site was approximately 40-fold higher than that of free Cy5.5.
34637934	7	21	theme	ZIF-8-Dex	1495:1503	arg1	higher					1545:1550	higher	1545:1550	higher	1545:1550	Most impressively, the fluorescent intensity of Cy5.5&ICG@ZIF-8-Dex at tumor site was approximately 40-fold higher than that of free Cy5.5.
34637934	9	22	theme	biomedical	2099:2108	arg1	field					2110:2114	the biomedical field	2095:2114	the biomedical field	2095:2114	Therefore, this multifunctional system based on ZIF-8 nanocarriers offered a potential nanoplatform for tumor-targeting theranostics, thus broadening the synthesis and applications of ZIF-8 composite nanoparticles for NIR fluorescence imaging and photothermal therapy in the biomedical field.
34637934	3	23	theme	hydrophilic	828:838	arg1	green					852:856	hydrophilic indocyanine green	828:856	hydrophilic indocyanine green (ICG)	828:862	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	3	23	theme	hydrophilic	828:838	arg1	ICG					859:861	ICG	859:861	ICG	859:861	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	5	24	theme	imaging	1143:1149	arg1	capability					1151:1160	fluorescence imaging capability	1130:1160	fluorescence imaging capability	1130:1160	Moreover, the encapsulation of Cy5.5 and ICG within ZIF-8 nanoparticles endowed them with fluorescence imaging capability and photothermal conversion capacity, respectively.
34637934	3	25	theme	synthesis/encapsulation	612:634	arg1	two-in-one					636:645	synthesis/encapsulation two-in-one	612:645	a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step	606:919	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	12	26	theme	photothermal	2766:2777	arg1	capacity					2790:2797	photothermal conversion capacity	2766:2797	photothermal conversion capacity	2766:2797	The results showed that the co-loading of Cy5.5 and ICG within the ZIF-8 nanoparticles endowed them with a remarkable fluorescence imaging capability and photothermal conversion capacity.
34637934	6	27	theme	sufficient	1333:1342	arg1	accumulations					1344:1356	sufficient accumulations	1333:1356	sufficient accumulations of Cy5.5 at tumor sites	1333:1380	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex demonstrated sufficient accumulations of Cy5.5 at tumor sites due to the enhanced permeability and retention effect.
34637934	10	28	theme	hydrophilic	2175:2185	arg1	molecules					2203:2211	hydrophilic and hydrophobic molecules	2175:2211	hydrophilic and hydrophobic molecules	2175:2211	STATEMENT OF SIGNIFICANCE: Simultaneous immobilization of hydrophilic and hydrophobic molecules into ZIF-8 nanoparticles still remains a daunting challenge.
34637934	6	29	theme	retention	1419:1427	arg1	effect					1429:1434	the enhanced permeability and retention effect	1389:1434	the enhanced permeability and retention effect	1389:1434	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex demonstrated sufficient accumulations of Cy5.5 at tumor sites due to the enhanced permeability and retention effect.
34637934	1	30	theme	imidazolate	244:254	arg1	ZIF-8					269:273	ZIF-8	269:273	ZIF-8	269:273	Impressive developments have been achieved with the use of zeolitic imidazolate framework-8 (ZIF-8) as nanocarriers for tumor theranostics in recent decades by incorporating imaging agents and therapeutic drugs within ZIF-8.
34637934	1	30	theme	imidazolate	244:254	arg1	framework-8					256:266	zeolitic imidazolate framework-8	235:266	zeolitic imidazolate framework-8 (ZIF-8)	235:274	Impressive developments have been achieved with the use of zeolitic imidazolate framework-8 (ZIF-8) as nanocarriers for tumor theranostics in recent decades by incorporating imaging agents and therapeutic drugs within ZIF-8.
34637934	9	31	theme	multifunctional	1840:1854	arg1	system					1856:1861	this multifunctional system	1835:1861	this multifunctional system based on ZIF-8 nanocarriers	1835:1889	Therefore, this multifunctional system based on ZIF-8 nanocarriers offered a potential nanoplatform for tumor-targeting theranostics, thus broadening the synthesis and applications of ZIF-8 composite nanoparticles for NIR fluorescence imaging and photothermal therapy in the biomedical field.
34637934	6	32	theme	permeability	1402:1413	arg1	effect					1429:1434	the enhanced permeability and retention effect	1389:1434	the enhanced permeability and retention effect	1389:1434	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex demonstrated sufficient accumulations of Cy5.5 at tumor sites due to the enhanced permeability and retention effect.
34637934	12	33	dep	nanoparticles	2685:2697	arg1	endowed					2699:2705	endowed	2699:2705	endowed	2699:2705	The results showed that the co-loading of Cy5.5 and ICG within the ZIF-8 nanoparticles endowed them with a remarkable fluorescence imaging capability and photothermal conversion capacity.
34637934	6	34	theme	Cy5.5&ICG	1300:1308	arg1	ZIF-8-Dex					1310:1318	Cy5.5&ICG@ZIF-8-Dex	1300:1318	Cy5.5&ICG@ZIF-8-Dex	1300:1318	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex demonstrated sufficient accumulations of Cy5.5 at tumor sites due to the enhanced permeability and retention effect.
34637934	3	35	theme	uniform	691:697	arg1	nanoparticles					736:748	uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles	691:748	uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step	691:919	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	9	36	theme	ZIF-8	1872:1876	arg1	nanocarriers					1878:1889	ZIF-8 nanocarriers	1872:1889	ZIF-8 nanocarriers	1872:1889	Therefore, this multifunctional system based on ZIF-8 nanocarriers offered a potential nanoplatform for tumor-targeting theranostics, thus broadening the synthesis and applications of ZIF-8 composite nanoparticles for NIR fluorescence imaging and photothermal therapy in the biomedical field.
34637934	13	37	theme	potential	3011:3019	arg1	nanoplatform					3021:3032	a potential nanoplatform	3009:3032	a potential nanoplatform toward fluorescence imaging and photothermal therapy for tumor theranostics	3009:3108	Based on their enhanced convenience and efficacy to simultaneously encapsulate hydrophilic and hydrophobic molecules, the multifunctional nanocarriers that were prepared in the DMSO/H2O mixed solvents provide a potential nanoplatform toward fluorescence imaging and photothermal therapy for tumor theranostics.
34637934	3	38	theme	cyanine-5.5	880:890	arg1	encapsulation					811:823	the simultaneous encapsulation	794:823	the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5)	794:898	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	4	39	from	approach	956:963	arg1	solvents					979:986	this mixed solvents	968:986	this mixed solvents	968:986	It was confirmed that the one-pot approach in this mixed solvents facilitated the loading of ICG and Cy5.5 molecules.
34637934	11	40	theme	Cy5.5&ICG	2375:2383	arg1	nanoparticles					2405:2417	uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles	2367:2417	uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures	2367:2446	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	1	41	theme	Impressive	176:185	arg1	developments					187:198	Impressive developments	176:198	Impressive developments	176:198	Impressive developments have been achieved with the use of zeolitic imidazolate framework-8 (ZIF-8) as nanocarriers for tumor theranostics in recent decades by incorporating imaging agents and therapeutic drugs within ZIF-8.
34637934	11	42	theme	solvent	2431:2437	arg1	mixtures					2439:2446	DMSO/H2O solvent mixtures	2422:2446	DMSO/H2O solvent mixtures	2422:2446	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	3	43	theme	same	911:914	arg1	step					916:919	the same step	907:919	the same step	907:919	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	0	44	theme	functional	84:93	arg1	cargos					95:100	hydrophilic and hydrophobic functional cargos	56:100	hydrophilic and hydrophobic functional cargos for combined near-infrared fluorescence imaging and photothermal therapy	56:173	Monodisperse ZIF-8@dextran nanoparticles co-loaded with hydrophilic and hydrophobic functional cargos for combined near-infrared fluorescence imaging and photothermal therapy.
34637934	13	45	theme	tumor	3091:3095	arg1	theranostics					3097:3108	tumor theranostics	3091:3108	tumor theranostics	3091:3108	Based on their enhanced convenience and efficacy to simultaneously encapsulate hydrophilic and hydrophobic molecules, the multifunctional nanocarriers that were prepared in the DMSO/H2O mixed solvents provide a potential nanoplatform toward fluorescence imaging and photothermal therapy for tumor theranostics.
34637934	9	46	theme	potential	1901:1909	arg1	nanoplatform					1911:1922	a potential nanoplatform	1899:1922	a potential nanoplatform for tumor-targeting theranostics	1899:1955	Therefore, this multifunctional system based on ZIF-8 nanocarriers offered a potential nanoplatform for tumor-targeting theranostics, thus broadening the synthesis and applications of ZIF-8 composite nanoparticles for NIR fluorescence imaging and photothermal therapy in the biomedical field.
34637934	12	47	with	endowed	2699:2705	arg1	capacity					2790:2797	photothermal conversion capacity	2766:2797	photothermal conversion capacity	2766:2797	The results showed that the co-loading of Cy5.5 and ICG within the ZIF-8 nanoparticles endowed them with a remarkable fluorescence imaging capability and photothermal conversion capacity.
34637934	12	47	with	endowed	2699:2705	arg1	capability					2751:2760	a remarkable fluorescence imaging capability	2717:2760	a remarkable fluorescence imaging capability	2717:2760	The results showed that the co-loading of Cy5.5 and ICG within the ZIF-8 nanoparticles endowed them with a remarkable fluorescence imaging capability and photothermal conversion capacity.
34637934	13	48	theme	hydrophilic	2879:2889	arg1	molecules					2907:2915	hydrophilic and hydrophobic molecules	2879:2915	hydrophilic and hydrophobic molecules	2879:2915	Based on their enhanced convenience and efficacy to simultaneously encapsulate hydrophilic and hydrophobic molecules, the multifunctional nanocarriers that were prepared in the DMSO/H2O mixed solvents provide a potential nanoplatform toward fluorescence imaging and photothermal therapy for tumor theranostics.
34637934	5	49	theme	conversion	1179:1188	arg1	capacity					1190:1197	photothermal conversion capacity	1166:1197	photothermal conversion capacity	1166:1197	Moreover, the encapsulation of Cy5.5 and ICG within ZIF-8 nanoparticles endowed them with fluorescence imaging capability and photothermal conversion capacity, respectively.
34637934	11	50	theme	single	2599:2604	arg1	step					2606:2609	a single step	2597:2609	a single step	2597:2609	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	0	51	theme	near-infrared	115:127	arg1	imaging					142:148	combined near-infrared fluorescence imaging	106:148	combined near-infrared fluorescence imaging	106:148	Monodisperse ZIF-8@dextran nanoparticles co-loaded with hydrophilic and hydrophobic functional cargos for combined near-infrared fluorescence imaging and photothermal therapy.
34637934	11	52	theme	@	2384:2384	arg1	nanoparticles					2405:2417	uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles	2367:2417	uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures	2367:2446	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	8	53	theme	imaging	1623:1629	arg1	results					1595:1601	the results	1591:1601	the results of in vivo infrared imaging and photothermal therapy of Cy5.5&ICG@ZIF-8-Dex	1591:1677	Additionally, the results of in vivo infrared imaging and photothermal therapy of Cy5.5&ICG@ZIF-8-Dex showed enhanced therapeutic efficiency in comparison with free ICG, further confirming its tumor-targeting capability and photothermal capacity.
34637934	11	54	theme	simultaneous	2467:2478	arg1	encapsulation					2480:2492	the simultaneous encapsulation	2463:2492	the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules	2463:2588	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	2	55	theme	simultaneous	414:425	arg1	immobilization					427:440	the simultaneous immobilization	410:440	the simultaneous immobilization of hydrophilic and hydrophobic functional molecules into ZIF-8 nanoparticles in water or organic solvents	410:546	However, the simultaneous immobilization of hydrophilic and hydrophobic functional molecules into ZIF-8 nanoparticles in water or organic solvents still presents a daunting challenge.
34637934	0	56	theme	Monodisperse	0:11	arg1	dextran					19:25	Monodisperse ZIF-8@dextran	0:25	Monodisperse ZIF-8@dextran	0:25	Monodisperse ZIF-8@dextran nanoparticles co-loaded with hydrophilic and hydrophobic functional cargos for combined near-infrared fluorescence imaging and photothermal therapy.
34637934	13	57	theme	fluorescence	3041:3052	arg1	imaging					3054:3060	fluorescence imaging	3041:3060	fluorescence imaging	3041:3060	Based on their enhanced convenience and efficacy to simultaneously encapsulate hydrophilic and hydrophobic molecules, the multifunctional nanocarriers that were prepared in the DMSO/H2O mixed solvents provide a potential nanoplatform toward fluorescence imaging and photothermal therapy for tumor theranostics.
34637934	11	58	theme	uniform	2367:2373	arg1	nanoparticles					2405:2417	uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles	2367:2417	uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures	2367:2446	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	8	59	theme	photothermal	1635:1646	arg1	therapy					1648:1654	photothermal therapy	1635:1654	photothermal therapy of Cy5.5&ICG@ZIF-8-Dex	1635:1677	Additionally, the results of in vivo infrared imaging and photothermal therapy of Cy5.5&ICG@ZIF-8-Dex showed enhanced therapeutic efficiency in comparison with free ICG, further confirming its tumor-targeting capability and photothermal capacity.
34637934	2	60	theme	functional	473:482	arg1	molecules					484:492	hydrophilic and hydrophobic functional molecules	445:492	hydrophilic and hydrophobic functional molecules into ZIF-8 nanoparticles	445:517	However, the simultaneous immobilization of hydrophilic and hydrophobic functional molecules into ZIF-8 nanoparticles in water or organic solvents still presents a daunting challenge.
34637934	0	61	theme	@	18:18	arg1	dextran					19:25	Monodisperse ZIF-8@dextran	0:25	Monodisperse ZIF-8@dextran	0:25	Monodisperse ZIF-8@dextran nanoparticles co-loaded with hydrophilic and hydrophobic functional cargos for combined near-infrared fluorescence imaging and photothermal therapy.
34637934	1	62	theme	recent	318:323	arg1	decades					325:331	recent decades	318:331	recent decades	318:331	Impressive developments have been achieved with the use of zeolitic imidazolate framework-8 (ZIF-8) as nanocarriers for tumor theranostics in recent decades by incorporating imaging agents and therapeutic drugs within ZIF-8.
34637934	2	63	theme	organic	531:537	arg1	solvents					539:546	organic solvents	531:546	organic solvents	531:546	However, the simultaneous immobilization of hydrophilic and hydrophobic functional molecules into ZIF-8 nanoparticles in water or organic solvents still presents a daunting challenge.
34637934	11	64	theme	green	2521:2525	arg1	encapsulation					2480:2492	the simultaneous encapsulation	2463:2492	the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules	2463:2588	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	3	65	theme	ZIF-8-Dex	726:734	arg1	nanoparticles					736:748	uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles	691:748	uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step	691:919	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	10	66	theme	hydrophobic	2191:2201	arg1	molecules					2203:2211	hydrophilic and hydrophobic molecules	2175:2211	hydrophilic and hydrophobic molecules	2175:2211	STATEMENT OF SIGNIFICANCE: Simultaneous immobilization of hydrophilic and hydrophobic molecules into ZIF-8 nanoparticles still remains a daunting challenge.
34637934	11	67	theme	composite	2395:2403	arg1	nanoparticles					2405:2417	uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles	2367:2417	uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures	2367:2446	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	8	68	theme	@	1668:1668	arg1	ZIF-8-Dex					1669:1677	Cy5.5&ICG@ZIF-8-Dex	1659:1677	Cy5.5&ICG@ZIF-8-Dex	1659:1677	Additionally, the results of in vivo infrared imaging and photothermal therapy of Cy5.5&ICG@ZIF-8-Dex showed enhanced therapeutic efficiency in comparison with free ICG, further confirming its tumor-targeting capability and photothermal capacity.
34637934	3	69	theme	Cy5.5&ICG	716:724	arg1	nanoparticles					736:748	uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles	691:748	uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step	691:919	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	1	70	theme	imaging	350:356	arg1	agents					358:363	imaging agents	350:363	imaging agents	350:363	Impressive developments have been achieved with the use of zeolitic imidazolate framework-8 (ZIF-8) as nanocarriers for tumor theranostics in recent decades by incorporating imaging agents and therapeutic drugs within ZIF-8.
34637934	0	71	dep	nanoparticles	27:39	arg1	co-loaded					41:49	co-loaded	41:49	nanoparticles co-loaded with hydrophilic and hydrophobic functional cargos for combined near-infrared fluorescence imaging and photothermal therapy	27:173	Monodisperse ZIF-8@dextran nanoparticles co-loaded with hydrophilic and hydrophobic functional cargos for combined near-infrared fluorescence imaging and photothermal therapy.
34637934	6	72	theme	in	1218:1219	arg1	images					1258:1263	The in vivo near-infrared (NIR) fluorescent images	1214:1263	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex	1214:1318	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex demonstrated sufficient accumulations of Cy5.5 at tumor sites due to the enhanced permeability and retention effect.
34637934	12	73	theme	conversion	2779:2788	arg1	capacity					2790:2797	photothermal conversion capacity	2766:2797	photothermal conversion capacity	2766:2797	The results showed that the co-loading of Cy5.5 and ICG within the ZIF-8 nanoparticles endowed them with a remarkable fluorescence imaging capability and photothermal conversion capacity.
34637934	3	74	theme	solvent	762:768	arg1	mixtures					770:777	DMSO/H2O solvent mixtures	753:777	DMSO/H2O solvent mixtures	753:777	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	6	75	from	sites	1376:1380	arg1	accumulations					1344:1356	sufficient accumulations	1333:1356	sufficient accumulations of Cy5.5 at tumor sites	1333:1380	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex demonstrated sufficient accumulations of Cy5.5 at tumor sites due to the enhanced permeability and retention effect.
34637934	9	76	theme	tumor-targeting	1928:1942	arg1	theranostics					1944:1955	tumor-targeting theranostics	1928:1955	tumor-targeting theranostics	1928:1955	Therefore, this multifunctional system based on ZIF-8 nanocarriers offered a potential nanoplatform for tumor-targeting theranostics, thus broadening the synthesis and applications of ZIF-8 composite nanoparticles for NIR fluorescence imaging and photothermal therapy in the biomedical field.
34637934	9	77	theme	composite	2014:2022	arg1	nanoparticles					2024:2036	ZIF-8 composite nanoparticles	2008:2036	ZIF-8 composite nanoparticles for NIR fluorescence imaging and photothermal therapy	2008:2090	Therefore, this multifunctional system based on ZIF-8 nanocarriers offered a potential nanoplatform for tumor-targeting theranostics, thus broadening the synthesis and applications of ZIF-8 composite nanoparticles for NIR fluorescence imaging and photothermal therapy in the biomedical field.
34637934	8	78	theme	therapeutic	1695:1705	arg1	efficiency					1707:1716	enhanced therapeutic efficiency	1686:1716	enhanced therapeutic efficiency	1686:1716	Additionally, the results of in vivo infrared imaging and photothermal therapy of Cy5.5&ICG@ZIF-8-Dex showed enhanced therapeutic efficiency in comparison with free ICG, further confirming its tumor-targeting capability and photothermal capacity.
34637934	3	79	from	nanoparticles	736:748	arg1	mixtures					770:777	DMSO/H2O solvent mixtures	753:777	DMSO/H2O solvent mixtures	753:777	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	2	80	theme	hydrophilic	445:455	arg1	molecules					484:492	hydrophilic and hydrophobic functional molecules	445:492	hydrophilic and hydrophobic functional molecules into ZIF-8 nanoparticles	445:517	However, the simultaneous immobilization of hydrophilic and hydrophobic functional molecules into ZIF-8 nanoparticles in water or organic solvents still presents a daunting challenge.
34637934	13	81	theme	enhanced	2815:2822	arg1	convenience					2824:2834	convenience	2824:2834	convenience	2824:2834	Based on their enhanced convenience and efficacy to simultaneously encapsulate hydrophilic and hydrophobic molecules, the multifunctional nanocarriers that were prepared in the DMSO/H2O mixed solvents provide a potential nanoplatform toward fluorescence imaging and photothermal therapy for tumor theranostics.
34637934	11	82	theme	molecules	2580:2588	arg1	encapsulation					2480:2492	the simultaneous encapsulation	2463:2492	the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules	2463:2588	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	11	83	from	nanoparticles	2405:2417	arg1	mixtures					2439:2446	DMSO/H2O solvent mixtures	2422:2446	DMSO/H2O solvent mixtures	2422:2446	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	8	84	dep	in	1606:1607	arg1	vivo					1609:1612	vivo	1609:1612	vivo	1609:1612	Additionally, the results of in vivo infrared imaging and photothermal therapy of Cy5.5&ICG@ZIF-8-Dex showed enhanced therapeutic efficiency in comparison with free ICG, further confirming its tumor-targeting capability and photothermal capacity.
34637934	11	85	theme	synthesis/encapsulation	2309:2331	arg1	approach					2344:2351	a new synthesis/encapsulation two-in-one approach	2303:2351	a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures	2303:2446	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	9	86	theme	fluorescence	2046:2057	arg1	imaging					2059:2065	NIR fluorescence imaging	2042:2065	NIR fluorescence imaging	2042:2065	Therefore, this multifunctional system based on ZIF-8 nanocarriers offered a potential nanoplatform for tumor-targeting theranostics, thus broadening the synthesis and applications of ZIF-8 composite nanoparticles for NIR fluorescence imaging and photothermal therapy in the biomedical field.
34637934	8	87	with	comparison	1721:1730	arg1	ICG					1742:1744	free ICG	1737:1744	free ICG	1737:1744	Additionally, the results of in vivo infrared imaging and photothermal therapy of Cy5.5&ICG@ZIF-8-Dex showed enhanced therapeutic efficiency in comparison with free ICG, further confirming its tumor-targeting capability and photothermal capacity.
34637934	8	88	theme	photothermal	1801:1812	arg1	capacity					1814:1821	photothermal capacity	1801:1821	photothermal capacity	1801:1821	Additionally, the results of in vivo infrared imaging and photothermal therapy of Cy5.5&ICG@ZIF-8-Dex showed enhanced therapeutic efficiency in comparison with free ICG, further confirming its tumor-targeting capability and photothermal capacity.
34637934	4	89	theme	one-pot	948:954	arg1	approach					956:963	the one-pot approach	944:963	the one-pot approach in this mixed solvents	944:986	It was confirmed that the one-pot approach in this mixed solvents facilitated the loading of ICG and Cy5.5 molecules.
34637934	11	90	theme	indocyanine	2509:2519	arg1	ICG					2528:2530	ICG	2528:2530	ICG	2528:2530	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	11	90	theme	indocyanine	2509:2519	arg1	green					2521:2525	hydrophilic indocyanine green	2497:2525	hydrophilic indocyanine green (ICG)	2497:2531	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	12	91	theme	remarkable	2719:2728	arg1	capability					2751:2760	a remarkable fluorescence imaging capability	2717:2760	a remarkable fluorescence imaging capability	2717:2760	The results showed that the co-loading of Cy5.5 and ICG within the ZIF-8 nanoparticles endowed them with a remarkable fluorescence imaging capability and photothermal conversion capacity.
34637934	11	92	theme	hydrophobic	2537:2547	arg1	molecules					2580:2588	hydrophobic cyanine-5.5 (Cy5.5) functional molecules	2537:2588	hydrophobic cyanine-5.5 (Cy5.5) functional molecules	2537:2588	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	6	93	theme	fluorescent	1246:1256	arg1	images					1258:1263	The in vivo near-infrared (NIR) fluorescent images	1214:1263	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex	1214:1318	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex demonstrated sufficient accumulations of Cy5.5 at tumor sites due to the enhanced permeability and retention effect.
34637934	12	94	theme	fluorescence	2730:2741	arg1	capability					2751:2760	a remarkable fluorescence imaging capability	2717:2760	a remarkable fluorescence imaging capability	2717:2760	The results showed that the co-loading of Cy5.5 and ICG within the ZIF-8 nanoparticles endowed them with a remarkable fluorescence imaging capability and photothermal conversion capacity.
34637934	5	95	dep	nanoparticles	1098:1110	arg1	endowed					1112:1118	endowed	1112:1118	endowed	1112:1118	Moreover, the encapsulation of Cy5.5 and ICG within ZIF-8 nanoparticles endowed them with fluorescence imaging capability and photothermal conversion capacity, respectively.
34637934	11	96	dep	developed	2293:2301	arg1	enabled					2455:2461	enabled	2455:2461	enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step	2455:2609	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	3	97	theme	simultaneous	798:809	arg1	encapsulation					811:823	the simultaneous encapsulation	794:823	the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5)	794:898	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	1	98	from	theranostics	302:313	arg1	decades					325:331	recent decades	318:331	recent decades	318:331	Impressive developments have been achieved with the use of zeolitic imidazolate framework-8 (ZIF-8) as nanocarriers for tumor theranostics in recent decades by incorporating imaging agents and therapeutic drugs within ZIF-8.
34637934	3	99	theme	two-in-one	636:645	arg1	approach					668:675	a new synthesis/encapsulation two-in-one (denoted as one-pot) approach	606:675	a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step	606:919	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	13	100	theme	mixed	2986:2990	arg1	solvents					2992:2999	the DMSO/H2O mixed solvents	2973:2999	the DMSO/H2O mixed solvents	2973:2999	Based on their enhanced convenience and efficacy to simultaneously encapsulate hydrophilic and hydrophobic molecules, the multifunctional nanocarriers that were prepared in the DMSO/H2O mixed solvents provide a potential nanoplatform toward fluorescence imaging and photothermal therapy for tumor theranostics.
34637934	6	101	theme	NIR	1241:1243	arg1	images					1258:1263	The in vivo near-infrared (NIR) fluorescent images	1214:1263	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex	1214:1318	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex demonstrated sufficient accumulations of Cy5.5 at tumor sites due to the enhanced permeability and retention effect.
34637934	10	102	theme	SIGNIFICANCE	2130:2141	arg1	STATEMENT					2117:2125	STATEMENT	2117:2125	STATEMENT OF SIGNIFICANCE	2117:2141	STATEMENT OF SIGNIFICANCE: Simultaneous immobilization of hydrophilic and hydrophobic molecules into ZIF-8 nanoparticles still remains a daunting challenge.
34637934	3	103	theme	new	608:610	arg1	approach					668:675	a new synthesis/encapsulation two-in-one (denoted as one-pot) approach	606:675	a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step	606:919	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	6	104	theme	near-infrared	1226:1238	arg1	images					1258:1263	The in vivo near-infrared (NIR) fluorescent images	1214:1263	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex	1214:1318	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex demonstrated sufficient accumulations of Cy5.5 at tumor sites due to the enhanced permeability and retention effect.
34637934	3	105	theme	indocyanine	840:850	arg1	green					852:856	hydrophilic indocyanine green	828:856	hydrophilic indocyanine green (ICG)	828:862	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	3	105	theme	indocyanine	840:850	arg1	ICG					859:861	ICG	859:861	ICG	859:861	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	1	106	theme	zeolitic	235:242	arg1	ZIF-8					269:273	ZIF-8	269:273	ZIF-8	269:273	Impressive developments have been achieved with the use of zeolitic imidazolate framework-8 (ZIF-8) as nanocarriers for tumor theranostics in recent decades by incorporating imaging agents and therapeutic drugs within ZIF-8.
34637934	1	106	theme	zeolitic	235:242	arg1	framework-8					256:266	zeolitic imidazolate framework-8	235:266	zeolitic imidazolate framework-8 (ZIF-8)	235:274	Impressive developments have been achieved with the use of zeolitic imidazolate framework-8 (ZIF-8) as nanocarriers for tumor theranostics in recent decades by incorporating imaging agents and therapeutic drugs within ZIF-8.
34637934	7	107	theme	@	1494:1494	arg1	ZIF-8-Dex					1495:1503	Cy5.5&ICG@ZIF-8-Dex	1485:1503	Cy5.5&ICG@ZIF-8-Dex	1485:1503	Most impressively, the fluorescent intensity of Cy5.5&ICG@ZIF-8-Dex at tumor site was approximately 40-fold higher than that of free Cy5.5.
34637934	3	108	theme	hydrophobic	868:878	arg1	Cy5.5					893:897	Cy5.5	893:897	Cy5.5	893:897	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	3	108	theme	hydrophobic	868:878	arg1	cyanine-5.5					880:890	hydrophobic cyanine-5.5	868:890	hydrophobic cyanine-5.5 (Cy5.5)	868:898	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	5	109	theme	photothermal	1166:1177	arg1	capacity					1190:1197	photothermal conversion capacity	1166:1197	photothermal conversion capacity	1166:1197	Moreover, the encapsulation of Cy5.5 and ICG within ZIF-8 nanoparticles endowed them with fluorescence imaging capability and photothermal conversion capacity, respectively.
34637934	6	110	theme	@	1309:1309	arg1	ZIF-8-Dex					1310:1318	Cy5.5&ICG@ZIF-8-Dex	1300:1318	Cy5.5&ICG@ZIF-8-Dex	1300:1318	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex demonstrated sufficient accumulations of Cy5.5 at tumor sites due to the enhanced permeability and retention effect.
34637934	10	111	theme	Simultaneous	2144:2155	arg1	immobilization					2157:2170	Simultaneous immobilization	2144:2170	Simultaneous immobilization of hydrophilic and hydrophobic molecules into ZIF-8 nanoparticles	2144:2236	STATEMENT OF SIGNIFICANCE: Simultaneous immobilization of hydrophilic and hydrophobic molecules into ZIF-8 nanoparticles still remains a daunting challenge.
34637934	6	112	theme	enhanced	1393:1400	arg1	effect					1429:1434	the enhanced permeability and retention effect	1389:1434	the enhanced permeability and retention effect	1389:1434	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex demonstrated sufficient accumulations of Cy5.5 at tumor sites due to the enhanced permeability and retention effect.
34637934	4	113	theme	mixed	973:977	arg1	solvents					979:986	this mixed solvents	968:986	this mixed solvents	968:986	It was confirmed that the one-pot approach in this mixed solvents facilitated the loading of ICG and Cy5.5 molecules.
34637934	7	114	theme	fluorescent	1460:1470	arg1	intensity					1472:1480	the fluorescent intensity	1456:1480	the fluorescent intensity of Cy5.5&ICG@ZIF-8-Dex at tumor site	1456:1517	Most impressively, the fluorescent intensity of Cy5.5&ICG@ZIF-8-Dex at tumor site was approximately 40-fold higher than that of free Cy5.5.
34637934	7	114	theme	fluorescent	1460:1470	arg1	higher					1545:1550	higher	1545:1550	higher	1545:1550	Most impressively, the fluorescent intensity of Cy5.5&ICG@ZIF-8-Dex at tumor site was approximately 40-fold higher than that of free Cy5.5.
34637934	10	115	theme	molecules	2203:2211	arg1	immobilization					2157:2170	Simultaneous immobilization	2144:2170	Simultaneous immobilization of hydrophilic and hydrophobic molecules into ZIF-8 nanoparticles	2144:2236	STATEMENT OF SIGNIFICANCE: Simultaneous immobilization of hydrophilic and hydrophobic molecules into ZIF-8 nanoparticles still remains a daunting challenge.
34637934	0	116	theme	hydrophobic	72:82	arg1	cargos					95:100	hydrophilic and hydrophobic functional cargos	56:100	hydrophilic and hydrophobic functional cargos for combined near-infrared fluorescence imaging and photothermal therapy	56:173	Monodisperse ZIF-8@dextran nanoparticles co-loaded with hydrophilic and hydrophobic functional cargos for combined near-infrared fluorescence imaging and photothermal therapy.
34637934	5	117	theme	fluorescence	1130:1141	arg1	capability					1151:1160	fluorescence imaging capability	1130:1160	fluorescence imaging capability	1130:1160	Moreover, the encapsulation of Cy5.5 and ICG within ZIF-8 nanoparticles endowed them with fluorescence imaging capability and photothermal conversion capacity, respectively.
34637934	7	118	from	site	1514:1517	arg1	intensity					1472:1480	the fluorescent intensity	1456:1480	the fluorescent intensity of Cy5.5&ICG@ZIF-8-Dex at tumor site	1456:1517	Most impressively, the fluorescent intensity of Cy5.5&ICG@ZIF-8-Dex at tumor site was approximately 40-fold higher than that of free Cy5.5.
34637934	7	118	from	site	1514:1517	arg1	higher					1545:1550	higher	1545:1550	higher	1545:1550	Most impressively, the fluorescent intensity of Cy5.5&ICG@ZIF-8-Dex at tumor site was approximately 40-fold higher than that of free Cy5.5.
34637934	6	119	theme	mice	1281:1284	arg1	images					1258:1263	The in vivo near-infrared (NIR) fluorescent images	1214:1263	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex	1214:1318	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex demonstrated sufficient accumulations of Cy5.5 at tumor sites due to the enhanced permeability and retention effect.
34637934	1	120	theme	framework-8	256:266	arg1	use					228:230	the use	224:230	the use of zeolitic imidazolate framework-8 (ZIF-8) as nanocarriers for tumor theranostics in recent decades	224:331	Impressive developments have been achieved with the use of zeolitic imidazolate framework-8 (ZIF-8) as nanocarriers for tumor theranostics in recent decades by incorporating imaging agents and therapeutic drugs within ZIF-8.
34637934	1	121	theme	therapeutic	369:379	arg1	drugs					381:385	therapeutic drugs	369:385	therapeutic drugs	369:385	Impressive developments have been achieved with the use of zeolitic imidazolate framework-8 (ZIF-8) as nanocarriers for tumor theranostics in recent decades by incorporating imaging agents and therapeutic drugs within ZIF-8.
34637934	3	122	dep	two-in-one	636:645	arg1	denoted					648:654	denoted	648:654	denoted as one-pot	648:665	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	8	123	theme	in	1606:1607	arg1	imaging					1623:1629	in vivo infrared imaging	1606:1629	in vivo infrared imaging	1606:1629	Additionally, the results of in vivo infrared imaging and photothermal therapy of Cy5.5&ICG@ZIF-8-Dex showed enhanced therapeutic efficiency in comparison with free ICG, further confirming its tumor-targeting capability and photothermal capacity.
34637934	4	124	dep	ICG	1015:1017	arg1	molecules					1029:1037	molecules	1029:1037	molecules	1029:1037	It was confirmed that the one-pot approach in this mixed solvents facilitated the loading of ICG and Cy5.5 molecules.
34637934	0	125	theme	combined	106:113	arg1	imaging					142:148	combined near-infrared fluorescence imaging	106:148	combined near-infrared fluorescence imaging	106:148	Monodisperse ZIF-8@dextran nanoparticles co-loaded with hydrophilic and hydrophobic functional cargos for combined near-infrared fluorescence imaging and photothermal therapy.
34637934	4	126	theme	ICG	1015:1017	arg1	loading					1004:1010	the loading	1000:1010	the loading of ICG and Cy5.5 molecules	1000:1037	It was confirmed that the one-pot approach in this mixed solvents facilitated the loading of ICG and Cy5.5 molecules.
34637934	8	127	theme	infrared	1614:1621	arg1	imaging					1623:1629	in vivo infrared imaging	1606:1629	in vivo infrared imaging	1606:1629	Additionally, the results of in vivo infrared imaging and photothermal therapy of Cy5.5&ICG@ZIF-8-Dex showed enhanced therapeutic efficiency in comparison with free ICG, further confirming its tumor-targeting capability and photothermal capacity.
34637934	10	128	theme	ZIF-8	2218:2222	arg1	nanoparticles					2224:2236	ZIF-8 nanoparticles	2218:2236	ZIF-8 nanoparticles	2218:2236	STATEMENT OF SIGNIFICANCE: Simultaneous immobilization of hydrophilic and hydrophobic molecules into ZIF-8 nanoparticles still remains a daunting challenge.
34637934	0	129	theme	fluorescence	129:140	arg1	imaging					142:148	combined near-infrared fluorescence imaging	106:148	combined near-infrared fluorescence imaging	106:148	Monodisperse ZIF-8@dextran nanoparticles co-loaded with hydrophilic and hydrophobic functional cargos for combined near-infrared fluorescence imaging and photothermal therapy.
34637934	10	130	theme	daunting	2254:2261	arg1	challenge					2263:2271	a daunting challenge	2252:2271	a daunting challenge	2252:2271	STATEMENT OF SIGNIFICANCE: Simultaneous immobilization of hydrophilic and hydrophobic molecules into ZIF-8 nanoparticles still remains a daunting challenge.
34637934	2	131	theme	daunting	565:572	arg1	challenge					574:582	a daunting challenge	563:582	a daunting challenge	563:582	However, the simultaneous immobilization of hydrophilic and hydrophobic functional molecules into ZIF-8 nanoparticles in water or organic solvents still presents a daunting challenge.
34637934	5	132	with	endowed	1112:1118	arg1	capability					1151:1160	fluorescence imaging capability	1130:1160	fluorescence imaging capability	1130:1160	Moreover, the encapsulation of Cy5.5 and ICG within ZIF-8 nanoparticles endowed them with fluorescence imaging capability and photothermal conversion capacity, respectively.
34637934	5	132	with	endowed	1112:1118	arg1	capacity					1190:1197	photothermal conversion capacity	1166:1197	photothermal conversion capacity	1166:1197	Moreover, the encapsulation of Cy5.5 and ICG within ZIF-8 nanoparticles endowed them with fluorescence imaging capability and photothermal conversion capacity, respectively.
34637934	0	133	theme	ZIF-8	13:17	arg1	dextran					19:25	Monodisperse ZIF-8@dextran	0:25	Monodisperse ZIF-8@dextran	0:25	Monodisperse ZIF-8@dextran nanoparticles co-loaded with hydrophilic and hydrophobic functional cargos for combined near-infrared fluorescence imaging and photothermal therapy.
34637934	13	134	theme	multifunctional	2922:2936	arg1	nanocarriers					2938:2949	the multifunctional nanocarriers	2918:2949	the multifunctional nanocarriers that were prepared in the DMSO/H2O mixed solvents	2918:2999	Based on their enhanced convenience and efficacy to simultaneously encapsulate hydrophilic and hydrophobic molecules, the multifunctional nanocarriers that were prepared in the DMSO/H2O mixed solvents provide a potential nanoplatform toward fluorescence imaging and photothermal therapy for tumor theranostics.
34637934	13	135	theme	photothermal	3066:3077	arg1	therapy					3079:3085	photothermal therapy	3066:3085	photothermal therapy	3066:3085	Based on their enhanced convenience and efficacy to simultaneously encapsulate hydrophilic and hydrophobic molecules, the multifunctional nanocarriers that were prepared in the DMSO/H2O mixed solvents provide a potential nanoplatform toward fluorescence imaging and photothermal therapy for tumor theranostics.
34637934	2	136	theme	ZIF-8	499:503	arg1	nanoparticles					505:517	ZIF-8 nanoparticles	499:517	ZIF-8 nanoparticles	499:517	However, the simultaneous immobilization of hydrophilic and hydrophobic functional molecules into ZIF-8 nanoparticles in water or organic solvents still presents a daunting challenge.
34637934	8	137	theme	therapy	1648:1654	arg1	results					1595:1601	the results	1591:1601	the results of in vivo infrared imaging and photothermal therapy of Cy5.5&ICG@ZIF-8-Dex	1591:1677	Additionally, the results of in vivo infrared imaging and photothermal therapy of Cy5.5&ICG@ZIF-8-Dex showed enhanced therapeutic efficiency in comparison with free ICG, further confirming its tumor-targeting capability and photothermal capacity.
34637934	7	138	theme	Cy5.5&ICG	1485:1493	arg1	ZIF-8-Dex					1495:1503	Cy5.5&ICG@ZIF-8-Dex	1485:1503	Cy5.5&ICG@ZIF-8-Dex	1485:1503	Most impressively, the fluorescent intensity of Cy5.5&ICG@ZIF-8-Dex at tumor site was approximately 40-fold higher than that of free Cy5.5.
34637934	2	139	theme	molecules	484:492	arg1	immobilization					427:440	the simultaneous immobilization	410:440	the simultaneous immobilization of hydrophilic and hydrophobic functional molecules into ZIF-8 nanoparticles in water or organic solvents	410:546	However, the simultaneous immobilization of hydrophilic and hydrophobic functional molecules into ZIF-8 nanoparticles in water or organic solvents still presents a daunting challenge.
34637934	8	140	theme	Cy5.5&ICG	1659:1667	arg1	ZIF-8-Dex					1669:1677	Cy5.5&ICG@ZIF-8-Dex	1659:1677	Cy5.5&ICG@ZIF-8-Dex	1659:1677	Additionally, the results of in vivo infrared imaging and photothermal therapy of Cy5.5&ICG@ZIF-8-Dex showed enhanced therapeutic efficiency in comparison with free ICG, further confirming its tumor-targeting capability and photothermal capacity.
34637934	3	141	theme	@	725:725	arg1	nanoparticles					736:748	uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles	691:748	uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step	691:919	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	2	142	theme	hydrophobic	461:471	arg1	molecules					484:492	hydrophilic and hydrophobic functional molecules	445:492	hydrophilic and hydrophobic functional molecules into ZIF-8 nanoparticles	445:517	However, the simultaneous immobilization of hydrophilic and hydrophobic functional molecules into ZIF-8 nanoparticles in water or organic solvents still presents a daunting challenge.
34637934	5	143	theme	ICG	1081:1083	arg1	encapsulation					1054:1066	the encapsulation	1050:1066	the encapsulation of Cy5.5 and ICG within ZIF-8	1050:1096	Moreover, the encapsulation of Cy5.5 and ICG within ZIF-8 nanoparticles endowed them with fluorescence imaging capability and photothermal conversion capacity, respectively.
34637934	11	144	theme	cyanine-5.5	2549:2559	arg1	molecules					2580:2588	hydrophobic cyanine-5.5 (Cy5.5) functional molecules	2537:2588	hydrophobic cyanine-5.5 (Cy5.5) functional molecules	2537:2588	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	11	145	theme	DMSO/H2O	2422:2429	arg1	mixtures					2439:2446	DMSO/H2O solvent mixtures	2422:2446	DMSO/H2O solvent mixtures	2422:2446	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	8	146	theme	ZIF-8-Dex	1669:1677	arg1	imaging					1623:1629	in vivo infrared imaging	1606:1629	in vivo infrared imaging	1606:1629	Additionally, the results of in vivo infrared imaging and photothermal therapy of Cy5.5&ICG@ZIF-8-Dex showed enhanced therapeutic efficiency in comparison with free ICG, further confirming its tumor-targeting capability and photothermal capacity.
34637934	8	146	theme	ZIF-8-Dex	1669:1677	arg1	therapy					1648:1654	photothermal therapy	1635:1654	photothermal therapy of Cy5.5&ICG@ZIF-8-Dex	1635:1677	Additionally, the results of in vivo infrared imaging and photothermal therapy of Cy5.5&ICG@ZIF-8-Dex showed enhanced therapeutic efficiency in comparison with free ICG, further confirming its tumor-targeting capability and photothermal capacity.
34637934	3	147	theme	dextran-modified	699:714	arg1	nanoparticles					736:748	uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles	691:748	uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step	691:919	Herein, we developed a new synthesis/encapsulation two-in-one (denoted as one-pot) approach to synthesize uniform dextran-modified Cy5.5&ICG@ZIF-8-Dex nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) during the same step.
34637934	4	148	theme	Cy5.5	1023:1027	arg1	loading					1004:1010	the loading	1000:1010	the loading of ICG and Cy5.5 molecules	1000:1037	It was confirmed that the one-pot approach in this mixed solvents facilitated the loading of ICG and Cy5.5 molecules.
34637934	0	149	theme	hydrophilic	56:66	arg1	cargos					95:100	hydrophilic and hydrophobic functional cargos	56:100	hydrophilic and hydrophobic functional cargos for combined near-infrared fluorescence imaging and photothermal therapy	56:173	Monodisperse ZIF-8@dextran nanoparticles co-loaded with hydrophilic and hydrophobic functional cargos for combined near-infrared fluorescence imaging and photothermal therapy.
34637934	0	150	theme	photothermal	154:165	arg1	therapy					167:173	photothermal therapy	154:173	photothermal therapy	154:173	Monodisperse ZIF-8@dextran nanoparticles co-loaded with hydrophilic and hydrophobic functional cargos for combined near-infrared fluorescence imaging and photothermal therapy.
34637934	8	151	theme	enhanced	1686:1693	arg1	efficiency					1707:1716	enhanced therapeutic efficiency	1686:1716	enhanced therapeutic efficiency	1686:1716	Additionally, the results of in vivo infrared imaging and photothermal therapy of Cy5.5&ICG@ZIF-8-Dex showed enhanced therapeutic efficiency in comparison with free ICG, further confirming its tumor-targeting capability and photothermal capacity.
34637934	2	152	from	immobilization	427:440	arg1	water					522:526	water	522:526	water	522:526	However, the simultaneous immobilization of hydrophilic and hydrophobic functional molecules into ZIF-8 nanoparticles in water or organic solvents still presents a daunting challenge.
34637934	2	152	from	immobilization	427:440	arg1	solvents					539:546	organic solvents	531:546	organic solvents	531:546	However, the simultaneous immobilization of hydrophilic and hydrophobic functional molecules into ZIF-8 nanoparticles in water or organic solvents still presents a daunting challenge.
34637934	11	153	theme	Cy5.5	2562:2566	arg1	molecules					2580:2588	hydrophobic cyanine-5.5 (Cy5.5) functional molecules	2537:2588	hydrophobic cyanine-5.5 (Cy5.5) functional molecules	2537:2588	Therefore, we have developed a new synthesis/encapsulation two-in-one approach to synthesize uniform Cy5.5&ICG@ZIF-8-Dex composite nanoparticles in DMSO/H2O solvent mixtures, which enabled the simultaneous encapsulation of hydrophilic indocyanine green (ICG) and hydrophobic cyanine-5.5 (Cy5.5) functional molecules during a single step.
34637934	12	154	theme	Cy5.5	2654:2658	arg1	co-loading					2640:2649	the co-loading	2636:2649	the co-loading of Cy5.5 and ICG within the ZIF-8	2636:2683	The results showed that the co-loading of Cy5.5 and ICG within the ZIF-8 nanoparticles endowed them with a remarkable fluorescence imaging capability and photothermal conversion capacity.
34637934	8	155	theme	tumor-targeting	1770:1784	arg1	capability					1786:1795	tumor-targeting capability	1770:1795	tumor-targeting capability	1770:1795	Additionally, the results of in vivo infrared imaging and photothermal therapy of Cy5.5&ICG@ZIF-8-Dex showed enhanced therapeutic efficiency in comparison with free ICG, further confirming its tumor-targeting capability and photothermal capacity.
34637934	5	156	theme	Cy5.5	1071:1075	arg1	encapsulation					1054:1066	the encapsulation	1050:1066	the encapsulation of Cy5.5 and ICG within ZIF-8	1050:1096	Moreover, the encapsulation of Cy5.5 and ICG within ZIF-8 nanoparticles endowed them with fluorescence imaging capability and photothermal conversion capacity, respectively.
34637934	9	157	theme	nanoparticles	2024:2036	arg1	synthesis					1978:1986	synthesis	1978:1986	synthesis	1978:1986	Therefore, this multifunctional system based on ZIF-8 nanocarriers offered a potential nanoplatform for tumor-targeting theranostics, thus broadening the synthesis and applications of ZIF-8 composite nanoparticles for NIR fluorescence imaging and photothermal therapy in the biomedical field.
34637934	9	157	theme	nanoparticles	2024:2036	arg1	applications					1992:2003	applications	1992:2003	applications	1992:2003	Therefore, this multifunctional system based on ZIF-8 nanocarriers offered a potential nanoplatform for tumor-targeting theranostics, thus broadening the synthesis and applications of ZIF-8 composite nanoparticles for NIR fluorescence imaging and photothermal therapy in the biomedical field.
34637934	6	158	dep	in	1218:1219	arg1	vivo					1221:1224	vivo	1221:1224	vivo	1221:1224	The in vivo near-infrared (NIR) fluorescent images of A549-bearing mice injected with Cy5.5&ICG@ZIF-8-Dex demonstrated sufficient accumulations of Cy5.5 at tumor sites due to the enhanced permeability and retention effect.
31908039	4	0	theme	FLT3	668:671	arg1	duplication					605:615	internal tandem duplication	589:615	internal tandem duplication (ITD)	589:621	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	4	0	theme	FLT3	668:671	arg1	WT					584:585	WT	584:585	WT	584:585	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	4	0	theme	FLT3	668:671	arg1	type					578:581	wild type	573:581	wild type (WT)	573:586	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	4	0	theme	FLT3	668:671	arg1	mutants					657:663	tyrosine kinase domain (TKD) mutants	628:663	tyrosine kinase domain (TKD) mutants of FLT3	628:671	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	4	0	theme	FLT3	668:671	arg1	ITD					618:620	ITD	618:620	ITD	618:620	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	1	1	theme	tyrosine	210:217	arg1	family					226:231	the class III receptor tyrosine kinase family	187:231	the class III receptor tyrosine kinase family	187:231	Fms-like tyrosine kinase 3 (FLT3) is a glycoprotein, that is a member of the class III receptor tyrosine kinase family.
31908039	1	2	gly	glycoprotein	153:164	arg1	glycoprotein					153:164	a glycoprotein	151:164	a glycoprotein	151:164	Fms-like tyrosine kinase 3 (FLT3) is a glycoprotein, that is a member of the class III receptor tyrosine kinase family.
31908039	1	2	gly	glycoprotein	153:164	arg1	kinase					132:137	Fms-like tyrosine kinase 3	114:139	Fms-like tyrosine kinase 3 (FLT3)	114:146	Fms-like tyrosine kinase 3 (FLT3) is a glycoprotein, that is a member of the class III receptor tyrosine kinase family.
31908039	1	2	gly	glycoprotein	153:164	arg1	member					177:182	a member	175:182	a member of the class III receptor tyrosine kinase family	175:231	Fms-like tyrosine kinase 3 (FLT3) is a glycoprotein, that is a member of the class III receptor tyrosine kinase family.
31908039	1	3	theme	family	226:231	arg1	glycoprotein					153:164	a glycoprotein	151:164	a glycoprotein	151:164	Fms-like tyrosine kinase 3 (FLT3) is a glycoprotein, that is a member of the class III receptor tyrosine kinase family.
31908039	1	3	theme	family	226:231	arg1	kinase					132:137	Fms-like tyrosine kinase 3	114:139	Fms-like tyrosine kinase 3 (FLT3)	114:146	Fms-like tyrosine kinase 3 (FLT3) is a glycoprotein, that is a member of the class III receptor tyrosine kinase family.
31908039	1	3	theme	family	226:231	arg1	member					177:182	a member	175:182	a member of the class III receptor tyrosine kinase family	175:231	Fms-like tyrosine kinase 3 (FLT3) is a glycoprotein, that is a member of the class III receptor tyrosine kinase family.
31908039	10	4	theme	ITD	1776:1778	arg1	mutant					1780:1785	the ITD mutant	1772:1785	the ITD mutant	1772:1785	Additionally, immunostaining with FLT3 showed that the proteins were mainly expressed on the cell surface in the KO cells, which is similar to FLT3-WT cells, but different from the ITD mutant.
31908039	2	5	theme	leukemia	275:282	arg1	patients					290:297	acute myeloid leukemia (AML) patients	261:297	acute myeloid leukemia (AML) patients	261:297	Approximately one-third of acute myeloid leukemia (AML) patients have mutations of this gene, and activation of the FLT3 downstream pathway plays an important role in both normal and malignant hematopoiesis.
31908039	12	6	from	effective	2062:2070	arg1	treatment					2079:2087	the treatment	2075:2087	the treatment of AML	2075:2094	Taken together, the present study clearly defines the regulatory function of core fucosylation in FLT3, which could provide a valuable direction for development of drugs could be effective in the treatment of AML.
31908039	12	7	theme	drugs	2047:2051	arg1	development					2032:2042	development	2032:2042	development of drugs could be effective in the treatment of AML	2032:2094	Taken together, the present study clearly defines the regulatory function of core fucosylation in FLT3, which could provide a valuable direction for development of drugs could be effective in the treatment of AML.
31908039	2	8	theme	acute	261:265	arg1	AML					285:287	AML	285:287	AML	285:287	Approximately one-third of acute myeloid leukemia (AML) patients have mutations of this gene, and activation of the FLT3 downstream pathway plays an important role in both normal and malignant hematopoiesis.
31908039	2	8	theme	acute	261:265	arg1	leukemia					275:282	acute myeloid leukemia	261:282	acute myeloid leukemia (AML) patients	261:297	Approximately one-third of acute myeloid leukemia (AML) patients have mutations of this gene, and activation of the FLT3 downstream pathway plays an important role in both normal and malignant hematopoiesis.
31908039	5	9	theme	WT	725:726	arg1	FLT3					738:741	either WT or mutant FLT3	718:741	either WT or mutant FLT3	718:741	Interestingly, expression of either WT or mutant FLT3 in Ba/F3 cells, an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell, greatly induced core fucosylation.
31908039	9	10	theme	fucosylation	1571:1582	arg1	inhibitor					1584:1592	specific fucosylation inhibitor	1562:1592	specific fucosylation inhibitor	1562:1592	Consistently, a tyrosine kinase inhibitor efficiently inhibited cell proliferation induced by Fut8KO or specific fucosylation inhibitor.
31908039	0	11	from	expression	79:88	arg1	system					106:111	a Ba/F3 cell system	93:111	a Ba/F3 cell system	93:111	Deficiency of core fucosylation activates cellular signaling dependent on FLT3 expression in a Ba/F3 cell system.
31908039	12	12	theme	fucosylation	1965:1976	arg1	function					1948:1955	the regulatory function	1933:1955	the regulatory function of core fucosylation in FLT3, which could provide a valuable direction for development of drugs could be effective in the treatment of AML	1933:2094	Taken together, the present study clearly defines the regulatory function of core fucosylation in FLT3, which could provide a valuable direction for development of drugs could be effective in the treatment of AML.
31908039	5	13	theme	mutant	731:736	arg1	FLT3					738:741	either WT or mutant FLT3	718:741	either WT or mutant FLT3	718:741	Interestingly, expression of either WT or mutant FLT3 in Ba/F3 cells, an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell, greatly induced core fucosylation.
31908039	1	14	theme	Fms-like	114:121	arg1	glycoprotein					153:164	a glycoprotein	151:164	a glycoprotein	151:164	Fms-like tyrosine kinase 3 (FLT3) is a glycoprotein, that is a member of the class III receptor tyrosine kinase family.
31908039	1	14	theme	Fms-like	114:121	arg1	kinase					132:137	Fms-like tyrosine kinase 3	114:139	Fms-like tyrosine kinase 3 (FLT3)	114:146	Fms-like tyrosine kinase 3 (FLT3) is a glycoprotein, that is a member of the class III receptor tyrosine kinase family.
31908039	1	14	theme	Fms-like	114:121	arg1	FLT3					142:145	FLT3	142:145	FLT3	142:145	Fms-like tyrosine kinase 3 (FLT3) is a glycoprotein, that is a member of the class III receptor tyrosine kinase family.
31908039	1	14	theme	Fms-like	114:121	arg1	member					177:182	a member	175:182	a member of the class III receptor tyrosine kinase family	175:231	Fms-like tyrosine kinase 3 (FLT3) is a glycoprotein, that is a member of the class III receptor tyrosine kinase family.
31908039	12	15	theme	regulatory	1937:1946	arg1	function					1948:1955	the regulatory function	1933:1955	the regulatory function of core fucosylation in FLT3, which could provide a valuable direction for development of drugs could be effective in the treatment of AML	1933:2094	Taken together, the present study clearly defines the regulatory function of core fucosylation in FLT3, which could provide a valuable direction for development of drugs could be effective in the treatment of AML.
31908039	5	16	theme	hematopoietic	793:805	arg1	cell					818:821	an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell	759:821	an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell	759:821	Interestingly, expression of either WT or mutant FLT3 in Ba/F3 cells, an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell, greatly induced core fucosylation.
31908039	5	16	theme	hematopoietic	793:805	arg1	cells					752:756	Ba/F3 cells	746:756	Ba/F3 cells	746:756	Interestingly, expression of either WT or mutant FLT3 in Ba/F3 cells, an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell, greatly induced core fucosylation.
31908039	6	17	theme	fucosylation	893:904	arg1	function					876:883	the function	872:883	the function of core fucosylation in FLT3-mediated signaling	872:931	To elucidate the function of core fucosylation in FLT3-mediated signaling, we used a CRISPR/Cas9 system to establish α1,6-fucosyltransferase (Fut8) knockout (KO) cells.
31908039	7	18	from	manner	1107:1112	arg1	cells					1125:1129	FLT3-WT cells	1117:1129	FLT3-WT cells	1117:1129	Surprisingly, the Fut8KO resulted in cell proliferation in an IL-3-independent manner in FLT3-WT cells, which was not observed in the parental cells, and suggested that this proliferation is dependent on FLT3 expression.
31908039	4	19	theme	tandem	598:603	arg1	duplication					605:615	internal tandem duplication	589:615	internal tandem duplication (ITD)	589:621	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	4	19	theme	tandem	598:603	arg1	ITD					618:620	ITD	618:620	ITD	618:620	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	8	20	dep	increased	1264:1272	arg1	neutralized					1404:1414	neutralized	1404:1414	could be completely neutralized by restoration with Fut8 in the KO cells	1384:1455	Fut8KO greatly increased cellular tyrosine phosphorylation levels, together with an activation of STAT5, AKT, and ERK signaling, which could be completely neutralized by restoration with Fut8 in the KO cells.
31908039	8	21	theme	ERK	1363:1365	arg1	signaling					1367:1375	ERK signaling	1363:1375	ERK signaling	1363:1375	Fut8KO greatly increased cellular tyrosine phosphorylation levels, together with an activation of STAT5, AKT, and ERK signaling, which could be completely neutralized by restoration with Fut8 in the KO cells.
31908039	7	22	located	observed	1146:1153	arg1	cells					1171:1175	the parental cells	1158:1175	the parental cells	1158:1175	Surprisingly, the Fut8KO resulted in cell proliferation in an IL-3-independent manner in FLT3-WT cells, which was not observed in the parental cells, and suggested that this proliferation is dependent on FLT3 expression.
31908039	7	22	located	observed	1146:1153	arg2	cells					1125:1129	FLT3-WT cells	1117:1129	FLT3-WT cells	1117:1129	Surprisingly, the Fut8KO resulted in cell proliferation in an IL-3-independent manner in FLT3-WT cells, which was not observed in the parental cells, and suggested that this proliferation is dependent on FLT3 expression.
31908039	5	23	theme	core	840:843	arg1	fucosylation					845:856	core fucosylation	840:856	core fucosylation	840:856	Interestingly, expression of either WT or mutant FLT3 in Ba/F3 cells, an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell, greatly induced core fucosylation.
31908039	12	24	dep	drugs	2047:2051	arg1	effective					2062:2070	effective	2062:2070	effective	2062:2070	Taken together, the present study clearly defines the regulatory function of core fucosylation in FLT3, which could provide a valuable direction for development of drugs could be effective in the treatment of AML.
31908039	12	25	theme	AML	2092:2094	arg1	treatment					2079:2087	the treatment	2075:2087	the treatment of AML	2075:2094	Taken together, the present study clearly defines the regulatory function of core fucosylation in FLT3, which could provide a valuable direction for development of drugs could be effective in the treatment of AML.
31908039	0	26	theme	cell	101:104	arg1	system					106:111	a Ba/F3 cell system	93:111	a Ba/F3 cell system	93:111	Deficiency of core fucosylation activates cellular signaling dependent on FLT3 expression in a Ba/F3 cell system.
31908039	6	27	used	used	937:940	arg2	we					934:935	we	934:935	we	934:935	To elucidate the function of core fucosylation in FLT3-mediated signaling, we used a CRISPR/Cas9 system to establish α1,6-fucosyltransferase (Fut8) knockout (KO) cells.
31908039	6	28	theme	CRISPR/Cas9	944:954	arg1	system					956:961	a CRISPR/Cas9 system	942:961	a CRISPR/Cas9 system	942:961	To elucidate the function of core fucosylation in FLT3-mediated signaling, we used a CRISPR/Cas9 system to establish α1,6-fucosyltransferase (Fut8) knockout (KO) cells.
31908039	6	29	from	function	876:883	arg1	signaling					923:931	FLT3-mediated signaling	909:931	FLT3-mediated signaling	909:931	To elucidate the function of core fucosylation in FLT3-mediated signaling, we used a CRISPR/Cas9 system to establish α1,6-fucosyltransferase (Fut8) knockout (KO) cells.
31908039	0	30	theme	FLT3	74:77	arg1	expression					79:88	FLT3 expression	74:88	FLT3 expression in a Ba/F3 cell system	74:111	Deficiency of core fucosylation activates cellular signaling dependent on FLT3 expression in a Ba/F3 cell system.
31908039	4	31	theme	kinase	637:642	arg1	domain					644:649	tyrosine kinase domain	628:649	tyrosine kinase domain (TKD) mutants of FLT3	628:671	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	4	31	theme	kinase	637:642	arg1	TKD					652:654	TKD	652:654	TKD	652:654	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	2	32	theme	pathway	366:372	arg1	activation					332:341	activation	332:341	activation of the FLT3 downstream pathway	332:372	Approximately one-third of acute myeloid leukemia (AML) patients have mutations of this gene, and activation of the FLT3 downstream pathway plays an important role in both normal and malignant hematopoiesis.
31908039	0	33	theme	core	14:17	arg1	fucosylation					19:30	core fucosylation	14:30	core fucosylation	14:30	Deficiency of core fucosylation activates cellular signaling dependent on FLT3 expression in a Ba/F3 cell system.
31908039	2	34	theme	FLT3	350:353	arg1	pathway					366:372	the FLT3 downstream pathway	346:372	the FLT3 downstream pathway	346:372	Approximately one-third of acute myeloid leukemia (AML) patients have mutations of this gene, and activation of the FLT3 downstream pathway plays an important role in both normal and malignant hematopoiesis.
31908039	6	35	theme	FLT3-mediated	909:921	arg1	signaling					923:931	FLT3-mediated signaling	909:931	FLT3-mediated signaling	909:931	To elucidate the function of core fucosylation in FLT3-mediated signaling, we used a CRISPR/Cas9 system to establish α1,6-fucosyltransferase (Fut8) knockout (KO) cells.
31908039	8	36	theme	cellular	1274:1281	arg1	phosphorylation					1292:1306	cellular tyrosine phosphorylation	1274:1306	cellular tyrosine phosphorylation levels	1274:1313	Fut8KO greatly increased cellular tyrosine phosphorylation levels, together with an activation of STAT5, AKT, and ERK signaling, which could be completely neutralized by restoration with Fut8 in the KO cells.
31908039	6	37	theme	knockout	1007:1014	arg1	cells					1021:1025	α1,6-fucosyltransferase (Fut8) knockout (KO) cells	976:1025	α1,6-fucosyltransferase (Fut8) knockout (KO) cells	976:1025	To elucidate the function of core fucosylation in FLT3-mediated signaling, we used a CRISPR/Cas9 system to establish α1,6-fucosyltransferase (Fut8) knockout (KO) cells.
31908039	10	38	theme	FLT3-WT	1738:1744	arg1	cells					1746:1750	FLT3-WT cells	1738:1750	FLT3-WT cells	1738:1750	Additionally, immunostaining with FLT3 showed that the proteins were mainly expressed on the cell surface in the KO cells, which is similar to FLT3-WT cells, but different from the ITD mutant.
31908039	8	39	theme	phosphorylation	1292:1306	arg1	levels					1308:1313	cellular tyrosine phosphorylation levels	1274:1313	cellular tyrosine phosphorylation levels	1274:1313	Fut8KO greatly increased cellular tyrosine phosphorylation levels, together with an activation of STAT5, AKT, and ERK signaling, which could be completely neutralized by restoration with Fut8 in the KO cells.
31908039	10	40	with	immunostaining	1609:1622	arg1	FLT3					1629:1632	FLT3	1629:1632	FLT3	1629:1632	Additionally, immunostaining with FLT3 showed that the proteins were mainly expressed on the cell surface in the KO cells, which is similar to FLT3-WT cells, but different from the ITD mutant.
31908039	8	41	theme	KO	1448:1449	arg1	cells					1451:1455	the KO cells	1444:1455	the KO cells	1444:1455	Fut8KO greatly increased cellular tyrosine phosphorylation levels, together with an activation of STAT5, AKT, and ERK signaling, which could be completely neutralized by restoration with Fut8 in the KO cells.
31908039	12	42	theme	valuable	2009:2016	arg1	direction					2018:2026	a valuable direction	2007:2026	a valuable direction for development of drugs could be effective in the treatment of AML	2007:2094	Taken together, the present study clearly defines the regulatory function of core fucosylation in FLT3, which could provide a valuable direction for development of drugs could be effective in the treatment of AML.
31908039	2	43	theme	gene	322:325	arg1	mutations					304:312	mutations	304:312	mutations of this gene	304:325	Approximately one-third of acute myeloid leukemia (AML) patients have mutations of this gene, and activation of the FLT3 downstream pathway plays an important role in both normal and malignant hematopoiesis.
31908039	1	44	theme	class	191:195	arg1	family					226:231	the class III receptor tyrosine kinase family	187:231	the class III receptor tyrosine kinase family	187:231	Fms-like tyrosine kinase 3 (FLT3) is a glycoprotein, that is a member of the class III receptor tyrosine kinase family.
31908039	9	45	theme	kinase	1483:1488	arg1	inhibitor					1490:1498	a tyrosine kinase inhibitor	1472:1498	a tyrosine kinase inhibitor	1472:1498	Consistently, a tyrosine kinase inhibitor efficiently inhibited cell proliferation induced by Fut8KO or specific fucosylation inhibitor.
31908039	8	46	from	restoration	1419:1429	arg1	cells					1451:1455	the KO cells	1444:1455	the KO cells	1444:1455	Fut8KO greatly increased cellular tyrosine phosphorylation levels, together with an activation of STAT5, AKT, and ERK signaling, which could be completely neutralized by restoration with Fut8 in the KO cells.
31908039	1	47	theme	receptor	201:208	arg1	family					226:231	the class III receptor tyrosine kinase family	187:231	the class III receptor tyrosine kinase family	187:231	Fms-like tyrosine kinase 3 (FLT3) is a glycoprotein, that is a member of the class III receptor tyrosine kinase family.
31908039	7	48	theme	FLT3-WT	1117:1123	arg1	cells					1125:1129	FLT3-WT cells	1117:1129	FLT3-WT cells	1117:1129	Surprisingly, the Fut8KO resulted in cell proliferation in an IL-3-independent manner in FLT3-WT cells, which was not observed in the parental cells, and suggested that this proliferation is dependent on FLT3 expression.
31908039	3	49	theme	FLT3	483:486	arg1	activation					488:497	FLT3 activation	483:497	FLT3 activation	483:497	However, the role of N-glycosylation for FLT3 activation remains unclear.
31908039	1	50	theme	kinase	219:224	arg1	family					226:231	the class III receptor tyrosine kinase family	187:231	the class III receptor tyrosine kinase family	187:231	Fms-like tyrosine kinase 3 (FLT3) is a glycoprotein, that is a member of the class III receptor tyrosine kinase family.
31908039	9	51	theme	cell	1522:1525	arg1	proliferation					1527:1539	cell proliferation	1522:1539	cell proliferation induced by Fut8KO or specific fucosylation inhibitor	1522:1592	Consistently, a tyrosine kinase inhibitor efficiently inhibited cell proliferation induced by Fut8KO or specific fucosylation inhibitor.
31908039	3	52	theme	N-glycosylation	463:477	arg1	role					455:458	the role	451:458	the role of N-glycosylation for FLT3 activation	451:497	However, the role of N-glycosylation for FLT3 activation remains unclear.
31908039	12	53	from	treatment	2079:2087	arg1	effective					2062:2070	effective	2062:2070	effective	2062:2070	Taken together, the present study clearly defines the regulatory function of core fucosylation in FLT3, which could provide a valuable direction for development of drugs could be effective in the treatment of AML.
31908039	5	54	theme	FLT3	738:741	arg1	expression					704:713	expression	704:713	expression of either WT or mutant FLT3 in Ba/F3 cells, an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell,	704:822	Interestingly, expression of either WT or mutant FLT3 in Ba/F3 cells, an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell, greatly induced core fucosylation.
31908039	5	55	theme	Ba/F3	746:750	arg1	cell					818:821	an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell	759:821	an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell	759:821	Interestingly, expression of either WT or mutant FLT3 in Ba/F3 cells, an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell, greatly induced core fucosylation.
31908039	5	55	theme	Ba/F3	746:750	arg1	cells					752:756	Ba/F3 cells	746:756	Ba/F3 cells	746:756	Interestingly, expression of either WT or mutant FLT3 in Ba/F3 cells, an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell, greatly induced core fucosylation.
31908039	2	56	theme	myeloid	267:273	arg1	AML					285:287	AML	285:287	AML	285:287	Approximately one-third of acute myeloid leukemia (AML) patients have mutations of this gene, and activation of the FLT3 downstream pathway plays an important role in both normal and malignant hematopoiesis.
31908039	2	56	theme	myeloid	267:273	arg1	leukemia					275:282	acute myeloid leukemia	261:282	acute myeloid leukemia (AML) patients	261:297	Approximately one-third of acute myeloid leukemia (AML) patients have mutations of this gene, and activation of the FLT3 downstream pathway plays an important role in both normal and malignant hematopoiesis.
31908039	9	57	theme	specific	1562:1569	arg1	inhibitor					1584:1592	specific fucosylation inhibitor	1562:1592	specific fucosylation inhibitor	1562:1592	Consistently, a tyrosine kinase inhibitor efficiently inhibited cell proliferation induced by Fut8KO or specific fucosylation inhibitor.
31908039	10	58	theme	cell	1688:1691	arg1	surface					1693:1699	the cell surface	1684:1699	the cell surface	1684:1699	Additionally, immunostaining with FLT3 showed that the proteins were mainly expressed on the cell surface in the KO cells, which is similar to FLT3-WT cells, but different from the ITD mutant.
31908039	4	59	from	structures	559:568	arg1	duplication					605:615	internal tandem duplication	589:615	internal tandem duplication (ITD)	589:621	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	4	59	from	structures	559:568	arg1	WT					584:585	WT	584:585	WT	584:585	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	4	59	from	structures	559:568	arg1	type					578:581	wild type	573:581	wild type (WT)	573:586	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	4	59	from	structures	559:568	arg1	mutants					657:663	tyrosine kinase domain (TKD) mutants	628:663	tyrosine kinase domain (TKD) mutants of FLT3	628:671	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	4	59	from	structures	559:568	arg1	ITD					618:620	ITD	618:620	ITD	618:620	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	4	60	theme	wild	573:576	arg1	WT					584:585	WT	584:585	WT	584:585	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	4	60	theme	wild	573:576	arg1	type					578:581	wild type	573:581	wild type (WT)	573:586	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	7	61	theme	cell	1065:1068	arg1	proliferation					1070:1082	cell proliferation	1065:1082	cell proliferation	1065:1082	Surprisingly, the Fut8KO resulted in cell proliferation in an IL-3-independent manner in FLT3-WT cells, which was not observed in the parental cells, and suggested that this proliferation is dependent on FLT3 expression.
31908039	10	62	from	mutant	1780:1785	arg1	different					1757:1765	different	1757:1765	different	1757:1765	Additionally, immunostaining with FLT3 showed that the proteins were mainly expressed on the cell surface in the KO cells, which is similar to FLT3-WT cells, but different from the ITD mutant.
31908039	8	63	theme	STAT5	1347:1351	arg1	activation					1333:1342	an activation	1330:1342	an activation of STAT5, AKT, and ERK signaling	1330:1375	Fut8KO greatly increased cellular tyrosine phosphorylation levels, together with an activation of STAT5, AKT, and ERK signaling, which could be completely neutralized by restoration with Fut8 in the KO cells.
31908039	7	64	theme	FLT3	1232:1235	arg1	expression					1237:1246	FLT3 expression	1232:1246	FLT3 expression	1232:1246	Surprisingly, the Fut8KO resulted in cell proliferation in an IL-3-independent manner in FLT3-WT cells, which was not observed in the parental cells, and suggested that this proliferation is dependent on FLT3 expression.
31908039	10	65	theme	KO	1708:1709	arg1	cells					1711:1715	the KO cells	1704:1715	the KO cells	1704:1715	Additionally, immunostaining with FLT3 showed that the proteins were mainly expressed on the cell surface in the KO cells, which is similar to FLT3-WT cells, but different from the ITD mutant.
31908039	10	65	theme	KO	1708:1709	arg1	similar					1727:1733	similar	1727:1733	similar	1727:1733	Additionally, immunostaining with FLT3 showed that the proteins were mainly expressed on the cell surface in the KO cells, which is similar to FLT3-WT cells, but different from the ITD mutant.
31908039	1	66	theme	tyrosine	123:130	arg1	glycoprotein					153:164	a glycoprotein	151:164	a glycoprotein	151:164	Fms-like tyrosine kinase 3 (FLT3) is a glycoprotein, that is a member of the class III receptor tyrosine kinase family.
31908039	1	66	theme	tyrosine	123:130	arg1	kinase					132:137	Fms-like tyrosine kinase 3	114:139	Fms-like tyrosine kinase 3 (FLT3)	114:146	Fms-like tyrosine kinase 3 (FLT3) is a glycoprotein, that is a member of the class III receptor tyrosine kinase family.
31908039	1	66	theme	tyrosine	123:130	arg1	FLT3					142:145	FLT3	142:145	FLT3	142:145	Fms-like tyrosine kinase 3 (FLT3) is a glycoprotein, that is a member of the class III receptor tyrosine kinase family.
31908039	1	66	theme	tyrosine	123:130	arg1	member					177:182	a member	175:182	a member of the class III receptor tyrosine kinase family	175:231	Fms-like tyrosine kinase 3 (FLT3) is a glycoprotein, that is a member of the class III receptor tyrosine kinase family.
31908039	8	67	theme	AKT	1354:1356	arg1	activation					1333:1342	an activation	1330:1342	an activation of STAT5, AKT, and ERK signaling	1330:1375	Fut8KO greatly increased cellular tyrosine phosphorylation levels, together with an activation of STAT5, AKT, and ERK signaling, which could be completely neutralized by restoration with Fut8 in the KO cells.
31908039	7	68	theme	IL-3-independent	1090:1105	arg1	manner					1107:1112	an IL-3-independent manner	1087:1112	an IL-3-independent manner in FLT3-WT cells, which was not observed in the parental cells, and suggested that this proliferation is dependent on FLT3 expression	1087:1246	Surprisingly, the Fut8KO resulted in cell proliferation in an IL-3-independent manner in FLT3-WT cells, which was not observed in the parental cells, and suggested that this proliferation is dependent on FLT3 expression.
31908039	6	69	theme	core	888:891	arg1	fucosylation					893:904	core fucosylation	888:904	core fucosylation	888:904	To elucidate the function of core fucosylation in FLT3-mediated signaling, we used a CRISPR/Cas9 system to establish α1,6-fucosyltransferase (Fut8) knockout (KO) cells.
31908039	4	70	theme	internal	589:596	arg1	duplication					605:615	internal tandem duplication	589:615	internal tandem duplication (ITD)	589:621	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	4	70	theme	internal	589:596	arg1	ITD					618:620	ITD	618:620	ITD	618:620	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	5	71	from	expression	704:713	arg1	cell					818:821	an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell	759:821	an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell	759:821	Interestingly, expression of either WT or mutant FLT3 in Ba/F3 cells, an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell, greatly induced core fucosylation.
31908039	5	71	from	expression	704:713	arg1	cells					752:756	Ba/F3 cells	746:756	Ba/F3 cells	746:756	Interestingly, expression of either WT or mutant FLT3 in Ba/F3 cells, an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell, greatly induced core fucosylation.
31908039	2	72	theme	malignant	417:425	arg1	hematopoiesis					427:439	both normal and malignant hematopoiesis	401:439	both normal and malignant hematopoiesis	401:439	Approximately one-third of acute myeloid leukemia (AML) patients have mutations of this gene, and activation of the FLT3 downstream pathway plays an important role in both normal and malignant hematopoiesis.
31908039	8	73	theme	signaling	1367:1375	arg1	activation					1333:1342	an activation	1330:1342	an activation of STAT5, AKT, and ERK signaling	1330:1375	Fut8KO greatly increased cellular tyrosine phosphorylation levels, together with an activation of STAT5, AKT, and ERK signaling, which could be completely neutralized by restoration with Fut8 in the KO cells.
31908039	12	74	theme	present	1903:1909	arg1	study					1911:1915	the present study	1899:1915	the present study	1899:1915	Taken together, the present study clearly defines the regulatory function of core fucosylation in FLT3, which could provide a valuable direction for development of drugs could be effective in the treatment of AML.
31908039	0	75	theme	Ba/F3	95:99	arg1	system					106:111	a Ba/F3 cell system	93:111	a Ba/F3 cell system	93:111	Deficiency of core fucosylation activates cellular signaling dependent on FLT3 expression in a Ba/F3 cell system.
31908039	2	76	contain	have	299:302	arg1	one-third					248:256	one-third	248:256	one-third	248:256	Approximately one-third of acute myeloid leukemia (AML) patients have mutations of this gene, and activation of the FLT3 downstream pathway plays an important role in both normal and malignant hematopoiesis.
31908039	2	76	contain	have	299:302	arg1	patients					290:297	acute myeloid leukemia (AML) patients	261:297	acute myeloid leukemia (AML) patients	261:297	Approximately one-third of acute myeloid leukemia (AML) patients have mutations of this gene, and activation of the FLT3 downstream pathway plays an important role in both normal and malignant hematopoiesis.
31908039	2	76	contain	have	299:302	arg2	mutations					304:312	mutations	304:312	mutations of this gene	304:325	Approximately one-third of acute myeloid leukemia (AML) patients have mutations of this gene, and activation of the FLT3 downstream pathway plays an important role in both normal and malignant hematopoiesis.
31908039	5	77	theme	-dependent	782:791	arg1	cell					818:821	an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell	759:821	an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell	759:821	Interestingly, expression of either WT or mutant FLT3 in Ba/F3 cells, an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell, greatly induced core fucosylation.
31908039	5	77	theme	-dependent	782:791	arg1	cells					752:756	Ba/F3 cells	746:756	Ba/F3 cells	746:756	Interestingly, expression of either WT or mutant FLT3 in Ba/F3 cells, an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell, greatly induced core fucosylation.
31908039	5	78	theme	progenitor	807:816	arg1	cell					818:821	an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell	759:821	an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell	759:821	Interestingly, expression of either WT or mutant FLT3 in Ba/F3 cells, an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell, greatly induced core fucosylation.
31908039	5	78	theme	progenitor	807:816	arg1	cells					752:756	Ba/F3 cells	746:756	Ba/F3 cells	746:756	Interestingly, expression of either WT or mutant FLT3 in Ba/F3 cells, an interleukin-3 (IL-3)-dependent hematopoietic progenitor cell, greatly induced core fucosylation.
31908039	2	79	theme	important	383:391	arg1	role					393:396	an important role	380:396	an important role	380:396	Approximately one-third of acute myeloid leukemia (AML) patients have mutations of this gene, and activation of the FLT3 downstream pathway plays an important role in both normal and malignant hematopoiesis.
31908039	4	80	theme	tyrosine	628:635	arg1	domain					644:649	tyrosine kinase domain	628:649	tyrosine kinase domain (TKD) mutants of FLT3	628:671	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	4	80	theme	tyrosine	628:635	arg1	TKD					652:654	TKD	652:654	TKD	652:654	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	0	81	theme	fucosylation	19:30	arg1	Deficiency					0:9	Deficiency	0:9	Deficiency of core fucosylation	0:30	Deficiency of core fucosylation activates cellular signaling dependent on FLT3 expression in a Ba/F3 cell system.
31908039	12	82	from	function	1948:1955	arg1	FLT3					1981:1984	FLT3	1981:1984	FLT3	1981:1984	Taken together, the present study clearly defines the regulatory function of core fucosylation in FLT3, which could provide a valuable direction for development of drugs could be effective in the treatment of AML.
31908039	8	83	with	restoration	1419:1429	arg1	Fut8					1436:1439	Fut8	1436:1439	Fut8	1436:1439	Fut8KO greatly increased cellular tyrosine phosphorylation levels, together with an activation of STAT5, AKT, and ERK signaling, which could be completely neutralized by restoration with Fut8 in the KO cells.
31908039	7	84	theme	parental	1162:1169	arg1	cells					1171:1175	the parental cells	1158:1175	the parental cells	1158:1175	Surprisingly, the Fut8KO resulted in cell proliferation in an IL-3-independent manner in FLT3-WT cells, which was not observed in the parental cells, and suggested that this proliferation is dependent on FLT3 expression.
31908039	4	85	theme	domain	644:649	arg1	mutants					657:663	tyrosine kinase domain (TKD) mutants	628:663	tyrosine kinase domain (TKD) mutants of FLT3	628:671	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	2	86	theme	downstream	355:364	arg1	pathway					366:372	the FLT3 downstream pathway	346:372	the FLT3 downstream pathway	346:372	Approximately one-third of acute myeloid leukemia (AML) patients have mutations of this gene, and activation of the FLT3 downstream pathway plays an important role in both normal and malignant hematopoiesis.
31908039	0	87	theme	cellular	42:49	arg1	signaling					51:59	cellular signaling	42:59	cellular signaling dependent on FLT3 expression in a Ba/F3 cell system	42:111	Deficiency of core fucosylation activates cellular signaling dependent on FLT3 expression in a Ba/F3 cell system.
31908039	2	88	theme	normal	406:411	arg1	hematopoiesis					427:439	both normal and malignant hematopoiesis	401:439	both normal and malignant hematopoiesis	401:439	Approximately one-third of acute myeloid leukemia (AML) patients have mutations of this gene, and activation of the FLT3 downstream pathway plays an important role in both normal and malignant hematopoiesis.
31908039	0	89	theme	dependent	61:69	arg1	signaling					51:59	cellular signaling	42:59	cellular signaling dependent on FLT3 expression in a Ba/F3 cell system	42:111	Deficiency of core fucosylation activates cellular signaling dependent on FLT3 expression in a Ba/F3 cell system.
31908039	2	90	theme	patients	290:297	arg1	one-third					248:256	one-third	248:256	one-third	248:256	Approximately one-third of acute myeloid leukemia (AML) patients have mutations of this gene, and activation of the FLT3 downstream pathway plays an important role in both normal and malignant hematopoiesis.
31908039	2	90	theme	patients	290:297	arg1	patients					290:297	acute myeloid leukemia (AML) patients	261:297	acute myeloid leukemia (AML) patients	261:297	Approximately one-third of acute myeloid leukemia (AML) patients have mutations of this gene, and activation of the FLT3 downstream pathway plays an important role in both normal and malignant hematopoiesis.
31908039	8	91	dep	together	1316:1323	arg1	with					1325:1328	with	1325:1328	with	1325:1328	Fut8KO greatly increased cellular tyrosine phosphorylation levels, together with an activation of STAT5, AKT, and ERK signaling, which could be completely neutralized by restoration with Fut8 in the KO cells.
31908039	8	92	theme	tyrosine	1283:1290	arg1	phosphorylation					1292:1306	cellular tyrosine phosphorylation	1274:1306	cellular tyrosine phosphorylation levels	1274:1313	Fut8KO greatly increased cellular tyrosine phosphorylation levels, together with an activation of STAT5, AKT, and ERK signaling, which could be completely neutralized by restoration with Fut8 in the KO cells.
31908039	12	93	theme	core	1960:1963	arg1	fucosylation					1965:1976	core fucosylation	1960:1976	core fucosylation	1960:1976	Taken together, the present study clearly defines the regulatory function of core fucosylation in FLT3, which could provide a valuable direction for development of drugs could be effective in the treatment of AML.
31908039	9	94	theme	tyrosine	1474:1481	arg1	inhibitor					1490:1498	a tyrosine kinase inhibitor	1472:1498	a tyrosine kinase inhibitor	1472:1498	Consistently, a tyrosine kinase inhibitor efficiently inhibited cell proliferation induced by Fut8KO or specific fucosylation inhibitor.
31908039	4	95	theme	N-glycan	550:557	arg1	different					678:686	different	678:686	different	678:686	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	4	95	theme	N-glycan	550:557	arg1	structures					559:568	the N-glycan structures	546:568	the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3	546:671	In this study, we showed that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.
31908039	6	96	dep	knockout	1007:1014	arg1	KO					1017:1018	KO	1017:1018	KO	1017:1018	To elucidate the function of core fucosylation in FLT3-mediated signaling, we used a CRISPR/Cas9 system to establish α1,6-fucosyltransferase (Fut8) knockout (KO) cells.
33389854	0	0	theme	Crude	70:74	arg1	Mushroom					22:29	Turkey Tail Medicinal Mushroom	0:29	Turkey Tail Medicinal Mushroom	0:29	Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity.
33389854	0	0	theme	Crude	70:74	arg1	Exopolysaccharides					76:93	Crude Exopolysaccharides	70:93	Crude Exopolysaccharides with Antioxidative Activity	70:121	Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity.
33389854	6	1	theme	amino	1020:1024	arg1	acid					1026:1029	bitter tasting amino acid	1005:1029	bitter tasting amino acid	1005:1029	In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.
33389854	5	2	theme	main	743:746	arg1	%					776:776	87.18 ± 0.27%	764:776	87.18 ± 0.27%	764:776	D-glucose was the main monosaccharide (87.18 ± 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate.
33389854	5	2	theme	main	743:746	arg1	monosaccharide					748:761	the main monosaccharide	739:761	the main monosaccharide (87.18 ± 0.27%)	739:777	D-glucose was the main monosaccharide (87.18 ± 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate.
33389854	5	2	theme	main	743:746	arg1	D-glucose					725:733	D-glucose	725:733	D-glucose	725:733	D-glucose was the main monosaccharide (87.18 ± 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate.
33389854	3	3	theme	cEPS	494:497	arg1	activities					476:485	antioxidative activities	462:485	antioxidative activities of the cEPS	462:497	However, based on the EC50 values, antioxidative activities of the cEPS decreased in the following order: reducing power > DPPH scavenging ability > chelating ability.
33389854	6	4	theme	2.1	1035:1037	arg1	times					1039:1043	times	1039:1043	times	1039:1043	In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.
33389854	6	5	theme	cEPS	1089:1092	arg1	fraction					1102:1109	cEPS protein fraction	1089:1109	cEPS protein fraction	1089:1109	In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.
33389854	3	6	theme	power	542:546	arg1	ability					566:572	reducing power > DPPH scavenging ability	533:572	reducing power > DPPH scavenging ability	533:572	However, based on the EC50 values, antioxidative activities of the cEPS decreased in the following order: reducing power > DPPH scavenging ability > chelating ability.
33389854	4	7	theme	antioxidative	699:711	arg1	activities					713:722	cEPS antioxidative activities	694:722	cEPS antioxidative activities	694:722	Due to the high carbohydrate and β-glucan content it is assumed that they are the main carriers of cEPS antioxidative activities.
33389854	5	8	theme	amino	868:872	arg1	L-lysine					815:822	L-lysine	815:822	L-lysine (L-glutamic and L-aspartic acid)	815:855	D-glucose was the main monosaccharide (87.18 ± 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate.
33389854	5	8	theme	amino	868:872	arg1	acids					874:878	amino acids	868:878	amino acids with taste similar to the monosodium glutamate	868:925	D-glucose was the main monosaccharide (87.18 ± 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate.
33389854	6	9	theme	fraction	1102:1109	arg1	composition					1074:1084	favorable composition	1064:1084	favorable composition of cEPS protein fraction for food industry applying	1064:1136	In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.
33389854	3	10	theme	antioxidative	462:474	arg1	activities					476:485	antioxidative activities	462:485	antioxidative activities of the cEPS	462:497	However, based on the EC50 values, antioxidative activities of the cEPS decreased in the following order: reducing power > DPPH scavenging ability > chelating ability.
33389854	4	11	theme	cEPS	694:697	arg1	activities					713:722	cEPS antioxidative activities	694:722	cEPS antioxidative activities	694:722	Due to the high carbohydrate and β-glucan content it is assumed that they are the main carriers of cEPS antioxidative activities.
33389854	2	12	theme	radical	283:289	arg1	scavenging					291:300	DPPH free radical scavenging	273:300	DPPH free radical scavenging	273:300	Obtained results revealed high ability of cEPS for DPPH free radical scavenging and high chelating ability at the highest tested concentration (20 mg/mL), while the reducing power was significantly lower.
33389854	2	13	theme	reducing	387:394	arg1	power					396:400	the reducing power	383:400	the reducing power	383:400	Obtained results revealed high ability of cEPS for DPPH free radical scavenging and high chelating ability at the highest tested concentration (20 mg/mL), while the reducing power was significantly lower.
33389854	2	13	theme	reducing	387:394	arg1	lower					420:424	lower	420:424	lower	420:424	Obtained results revealed high ability of cEPS for DPPH free radical scavenging and high chelating ability at the highest tested concentration (20 mg/mL), while the reducing power was significantly lower.
33389854	0	14	theme	Antioxidative	100:112	arg1	Activity					114:121	Antioxidative Activity	100:121	Antioxidative Activity	100:121	Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity.
33389854	2	15	theme	free	278:281	arg1	scavenging					291:300	DPPH free radical scavenging	273:300	DPPH free radical scavenging	273:300	Obtained results revealed high ability of cEPS for DPPH free radical scavenging and high chelating ability at the highest tested concentration (20 mg/mL), while the reducing power was significantly lower.
33389854	6	16	theme	tasting	1012:1018	arg1	acid					1026:1029	bitter tasting amino acid	1005:1029	bitter tasting amino acid	1005:1029	In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.
33389854	4	17	theme	β-glucan	628:635	arg1	content					637:643	the high carbohydrate and β-glucan content	602:643	content	637:643	Due to the high carbohydrate and β-glucan content it is assumed that they are the main carriers of cEPS antioxidative activities.
33389854	0	18	theme	Tail	7:10	arg1	Mushroom					22:29	Turkey Tail Medicinal Mushroom	0:29	Turkey Tail Medicinal Mushroom	0:29	Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity.
33389854	0	18	theme	Tail	7:10	arg1	versicolor					41:50	Trametes versicolor	32:50	Trametes versicolor (Agaricomycetes)	32:67	Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity.
33389854	0	18	theme	Tail	7:10	arg1	Exopolysaccharides					76:93	Crude Exopolysaccharides	70:93	Crude Exopolysaccharides with Antioxidative Activity	70:121	Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity.
33389854	3	19	theme	>	548:548	arg1	ability					566:572	reducing power > DPPH scavenging ability	533:572	reducing power > DPPH scavenging ability	533:572	However, based on the EC50 values, antioxidative activities of the cEPS decreased in the following order: reducing power > DPPH scavenging ability > chelating ability.
33389854	0	20	theme	Turkey	0:5	arg1	Mushroom					22:29	Turkey Tail Medicinal Mushroom	0:29	Turkey Tail Medicinal Mushroom	0:29	Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity.
33389854	0	20	theme	Turkey	0:5	arg1	versicolor					41:50	Trametes versicolor	32:50	Trametes versicolor (Agaricomycetes)	32:67	Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity.
33389854	0	20	theme	Turkey	0:5	arg1	Exopolysaccharides					76:93	Crude Exopolysaccharides	70:93	Crude Exopolysaccharides with Antioxidative Activity	70:121	Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity.
33389854	3	21	theme	DPPH	550:553	arg1	ability					566:572	reducing power > DPPH scavenging ability	533:572	reducing power > DPPH scavenging ability	533:572	However, based on the EC50 values, antioxidative activities of the cEPS decreased in the following order: reducing power > DPPH scavenging ability > chelating ability.
33389854	1	22	theme	antioxidative	190:202	arg1	testing					213:219	antioxidative activity testing	190:219	antioxidative activity testing	190:219	Crude Trametes versicolor exopolysaccharides (cEPS) were used for antioxidative activity testing.
33389854	5	23	with	acids	874:878	arg1	taste					885:889	taste	885:889	taste	885:889	D-glucose was the main monosaccharide (87.18 ± 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate.
33389854	2	24	theme	cEPS	264:267	arg1	ability					253:259	high ability	248:259	high ability of cEPS for DPPH free radical scavenging	248:300	Obtained results revealed high ability of cEPS for DPPH free radical scavenging and high chelating ability at the highest tested concentration (20 mg/mL), while the reducing power was significantly lower.
33389854	2	24	theme	cEPS	264:267	arg1	ability					321:327	high chelating ability	306:327	high chelating ability	306:327	Obtained results revealed high ability of cEPS for DPPH free radical scavenging and high chelating ability at the highest tested concentration (20 mg/mL), while the reducing power was significantly lower.
33389854	0	25	with	Exopolysaccharides	76:93	arg1	Activity					114:121	Antioxidative Activity	100:121	Antioxidative Activity	100:121	Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity.
33389854	2	26	theme	chelating	311:319	arg1	ability					321:327	high chelating ability	306:327	high chelating ability	306:327	Obtained results revealed high ability of cEPS for DPPH free radical scavenging and high chelating ability at the highest tested concentration (20 mg/mL), while the reducing power was significantly lower.
33389854	1	27	theme	activity	204:211	arg1	testing					213:219	antioxidative activity testing	190:219	antioxidative activity testing	190:219	Crude Trametes versicolor exopolysaccharides (cEPS) were used for antioxidative activity testing.
33389854	0	28	theme	Medicinal	12:20	arg1	Mushroom					22:29	Turkey Tail Medicinal Mushroom	0:29	Turkey Tail Medicinal Mushroom	0:29	Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity.
33389854	0	28	theme	Medicinal	12:20	arg1	versicolor					41:50	Trametes versicolor	32:50	Trametes versicolor (Agaricomycetes)	32:67	Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity.
33389854	0	28	theme	Medicinal	12:20	arg1	Exopolysaccharides					76:93	Crude Exopolysaccharides	70:93	Crude Exopolysaccharides with Antioxidative Activity	70:121	Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity.
33389854	5	29	theme	monosodium	906:915	arg1	glutamate					917:925	the monosodium glutamate	902:925	the monosodium glutamate	902:925	D-glucose was the main monosaccharide (87.18 ± 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate.
33389854	2	30	theme	high	306:309	arg1	ability					321:327	high chelating ability	306:327	high chelating ability	306:327	Obtained results revealed high ability of cEPS for DPPH free radical scavenging and high chelating ability at the highest tested concentration (20 mg/mL), while the reducing power was significantly lower.
33389854	5	31	theme	dominant	789:796	arg1	L-lysine					815:822	L-lysine	815:822	L-lysine (L-glutamic and L-aspartic acid)	815:855	D-glucose was the main monosaccharide (87.18 ± 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate.
33389854	5	31	theme	dominant	789:796	arg1	acids					804:808	the dominant amino acids	785:808	the dominant amino acids	785:808	D-glucose was the main monosaccharide (87.18 ± 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate.
33389854	0	32	theme	Trametes	32:39	arg1	Agaricomycetes					53:66	Agaricomycetes	53:66	Agaricomycetes	53:66	Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity.
33389854	0	32	theme	Trametes	32:39	arg1	Mushroom					22:29	Turkey Tail Medicinal Mushroom	0:29	Turkey Tail Medicinal Mushroom	0:29	Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity.
33389854	0	32	theme	Trametes	32:39	arg1	versicolor					41:50	Trametes versicolor	32:50	Trametes versicolor (Agaricomycetes)	32:67	Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity.
33389854	2	33	theme	tested	344:349	arg1	concentration					351:363	the highest tested concentration	332:363	the highest tested concentration (20 mg/mL)	332:374	Obtained results revealed high ability of cEPS for DPPH free radical scavenging and high chelating ability at the highest tested concentration (20 mg/mL), while the reducing power was significantly lower.
33389854	2	33	theme	tested	344:349	arg1	mg/mL					369:373	20 mg/mL	366:373	20 mg/mL	366:373	Obtained results revealed high ability of cEPS for DPPH free radical scavenging and high chelating ability at the highest tested concentration (20 mg/mL), while the reducing power was significantly lower.
33389854	6	34	theme	sweet	952:956	arg1	acids					972:976	sweet tasting amino acids	952:976	sweet tasting amino acids compared with the group of bitter tasting amino acid	952:1029	In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.
33389854	6	35	theme	acids	972:976	arg1	content					941:947	content	941:947	content of sweet tasting amino acids compared with the group of bitter tasting amino acid	941:1029	In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.
33389854	5	36	theme	L-glutamic	825:834	arg1	acid					851:854	L-glutamic and L-aspartic acid	825:854	L-glutamic and L-aspartic acid	825:854	D-glucose was the main monosaccharide (87.18 ± 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate.
33389854	5	36	theme	L-glutamic	825:834	arg1	L-lysine					815:822	L-lysine	815:822	L-lysine (L-glutamic and L-aspartic acid)	815:855	D-glucose was the main monosaccharide (87.18 ± 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate.
33389854	5	37	theme	amino	798:802	arg1	L-lysine					815:822	L-lysine	815:822	L-lysine (L-glutamic and L-aspartic acid)	815:855	D-glucose was the main monosaccharide (87.18 ± 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate.
33389854	5	37	theme	amino	798:802	arg1	acids					804:808	the dominant amino acids	785:808	the dominant amino acids	785:808	D-glucose was the main monosaccharide (87.18 ± 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate.
33389854	6	38	theme	bitter	1005:1010	arg1	acid					1026:1029	bitter tasting amino acid	1005:1029	bitter tasting amino acid	1005:1029	In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.
33389854	2	39	theme	high	248:251	arg1	ability					253:259	high ability	248:259	high ability of cEPS for DPPH free radical scavenging	248:300	Obtained results revealed high ability of cEPS for DPPH free radical scavenging and high chelating ability at the highest tested concentration (20 mg/mL), while the reducing power was significantly lower.
33389854	6	40	from	times	1039:1043	arg1	addition					931:938	addition	931:938	addition	931:938	In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.
33389854	6	41	theme	amino	966:970	arg1	acids					972:976	sweet tasting amino acids	952:976	sweet tasting amino acids compared with the group of bitter tasting amino acid	952:1029	In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.
33389854	3	42	theme	scavenging	555:564	arg1	ability					566:572	reducing power > DPPH scavenging ability	533:572	reducing power > DPPH scavenging ability	533:572	However, based on the EC50 values, antioxidative activities of the cEPS decreased in the following order: reducing power > DPPH scavenging ability > chelating ability.
33389854	4	43	theme	main	677:680	arg1	they					664:667	they	664:667	they	664:667	Due to the high carbohydrate and β-glucan content it is assumed that they are the main carriers of cEPS antioxidative activities.
33389854	4	43	theme	main	677:680	arg1	carriers					682:689	the main carriers	673:689	the main carriers of cEPS antioxidative activities	673:722	Due to the high carbohydrate and β-glucan content it is assumed that they are the main carriers of cEPS antioxidative activities.
33389854	5	44	theme	±	770:770	arg1	%					776:776	87.18 ± 0.27%	764:776	87.18 ± 0.27%	764:776	D-glucose was the main monosaccharide (87.18 ± 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate.
33389854	5	44	theme	±	770:770	arg1	monosaccharide					748:761	the main monosaccharide	739:761	the main monosaccharide (87.18 ± 0.27%)	739:777	D-glucose was the main monosaccharide (87.18 ± 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate.
33389854	2	45	theme	Obtained	222:229	arg1	results					231:237	Obtained results	222:237	Obtained results	222:237	Obtained results revealed high ability of cEPS for DPPH free radical scavenging and high chelating ability at the highest tested concentration (20 mg/mL), while the reducing power was significantly lower.
33389854	6	46	theme	favorable	1064:1072	arg1	composition					1074:1084	favorable composition	1064:1084	favorable composition of cEPS protein fraction for food industry applying	1064:1136	In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.
33389854	1	47	theme	Crude	124:128	arg1	versicolor					139:148	Crude Trametes versicolor	124:148	Crude Trametes versicolor exopolysaccharides (cEPS)	124:174	Crude Trametes versicolor exopolysaccharides (cEPS) were used for antioxidative activity testing.
33389854	6	48	theme	food	1115:1118	arg1	industry					1120:1127	food industry applying	1115:1136	food industry applying	1115:1136	In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.
33389854	6	49	dep	times	1039:1043	arg1	higher					1045:1050	higher	1045:1050	higher	1045:1050	In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.
33389854	2	50	theme	DPPH	273:276	arg1	scavenging					291:300	DPPH free radical scavenging	273:300	DPPH free radical scavenging	273:300	Obtained results revealed high ability of cEPS for DPPH free radical scavenging and high chelating ability at the highest tested concentration (20 mg/mL), while the reducing power was significantly lower.
33389854	3	51	theme	chelating	576:584	arg1	ability					586:592	chelating ability	576:592	chelating ability	576:592	However, based on the EC50 values, antioxidative activities of the cEPS decreased in the following order: reducing power > DPPH scavenging ability > chelating ability.
33389854	1	52	theme	Trametes	130:137	arg1	versicolor					139:148	Crude Trametes versicolor	124:148	Crude Trametes versicolor exopolysaccharides (cEPS)	124:174	Crude Trametes versicolor exopolysaccharides (cEPS) were used for antioxidative activity testing.
33389854	3	53	theme	reducing	533:540	arg1	ability					566:572	reducing power > DPPH scavenging ability	533:572	reducing power > DPPH scavenging ability	533:572	However, based on the EC50 values, antioxidative activities of the cEPS decreased in the following order: reducing power > DPPH scavenging ability > chelating ability.
33389854	6	54	theme	tasting	958:964	arg1	acids					972:976	sweet tasting amino acids	952:976	sweet tasting amino acids compared with the group of bitter tasting amino acid	952:1029	In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.
33389854	3	55	theme	following	516:524	arg1	order					526:530	the following order	512:530	the following order	512:530	However, based on the EC50 values, antioxidative activities of the cEPS decreased in the following order: reducing power > DPPH scavenging ability > chelating ability.
33389854	6	56	theme	applying	1129:1136	arg1	industry					1120:1127	food industry applying	1115:1136	food industry applying	1115:1136	In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.
33389854	4	57	theme	activities	713:722	arg1	they					664:667	they	664:667	they	664:667	Due to the high carbohydrate and β-glucan content it is assumed that they are the main carriers of cEPS antioxidative activities.
33389854	4	57	theme	activities	713:722	arg1	carriers					682:689	the main carriers	673:689	the main carriers of cEPS antioxidative activities	673:722	Due to the high carbohydrate and β-glucan content it is assumed that they are the main carriers of cEPS antioxidative activities.
33389854	1	58	theme	versicolor	139:148	arg1	cEPS					170:173	cEPS	170:173	cEPS	170:173	Crude Trametes versicolor exopolysaccharides (cEPS) were used for antioxidative activity testing.
33389854	1	58	theme	versicolor	139:148	arg1	exopolysaccharides					150:167	Crude Trametes versicolor exopolysaccharides	124:167	Crude Trametes versicolor exopolysaccharides (cEPS)	124:174	Crude Trametes versicolor exopolysaccharides (cEPS) were used for antioxidative activity testing.
33389854	2	59	theme	highest	336:342	arg1	concentration					351:363	the highest tested concentration	332:363	the highest tested concentration (20 mg/mL)	332:374	Obtained results revealed high ability of cEPS for DPPH free radical scavenging and high chelating ability at the highest tested concentration (20 mg/mL), while the reducing power was significantly lower.
33389854	2	59	theme	highest	336:342	arg1	mg/mL					369:373	20 mg/mL	366:373	20 mg/mL	366:373	Obtained results revealed high ability of cEPS for DPPH free radical scavenging and high chelating ability at the highest tested concentration (20 mg/mL), while the reducing power was significantly lower.
33389854	6	60	theme	acid	1026:1029	arg1	acid					1026:1029	bitter tasting amino acid	1005:1029	bitter tasting amino acid	1005:1029	In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.
33389854	6	60	theme	acid	1026:1029	arg1	group					996:1000	the group	992:1000	the group of bitter tasting amino acid	992:1029	In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.
33389854	1	61	used	used	181:184	arg2	cEPS					170:173	cEPS	170:173	cEPS	170:173	Crude Trametes versicolor exopolysaccharides (cEPS) were used for antioxidative activity testing.
33389854	1	61	used	used	181:184	arg2	exopolysaccharides					150:167	Crude Trametes versicolor exopolysaccharides	124:167	Crude Trametes versicolor exopolysaccharides (cEPS)	124:174	Crude Trametes versicolor exopolysaccharides (cEPS) were used for antioxidative activity testing.
33389854	3	62	theme	EC50	449:452	arg1	values					454:459	the EC50 values	445:459	the EC50 values	445:459	However, based on the EC50 values, antioxidative activities of the cEPS decreased in the following order: reducing power > DPPH scavenging ability > chelating ability.
33389854	5	63	theme	similar	891:897	arg1	L-lysine					815:822	L-lysine	815:822	L-lysine (L-glutamic and L-aspartic acid)	815:855	D-glucose was the main monosaccharide (87.18 ± 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate.
33389854	5	63	theme	similar	891:897	arg1	acids					874:878	amino acids	868:878	amino acids with taste similar to the monosodium glutamate	868:925	D-glucose was the main monosaccharide (87.18 ± 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate.
33389854	6	64	theme	protein	1094:1100	arg1	fraction					1102:1109	cEPS protein fraction	1089:1109	cEPS protein fraction	1089:1109	In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.
33389854	4	65	theme	high	606:609	arg1	carbohydrate					611:622	the high carbohydrate and β-glucan content	602:643	carbohydrate	611:622	Due to the high carbohydrate and β-glucan content it is assumed that they are the main carriers of cEPS antioxidative activities.
33389854	5	66	theme	L-aspartic	840:849	arg1	acid					851:854	L-glutamic and L-aspartic acid	825:854	L-glutamic and L-aspartic acid	825:854	D-glucose was the main monosaccharide (87.18 ± 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate.
33389854	5	66	theme	L-aspartic	840:849	arg1	L-lysine					815:822	L-lysine	815:822	L-lysine (L-glutamic and L-aspartic acid)	815:855	D-glucose was the main monosaccharide (87.18 ± 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate.
32532393	0	0	theme	strawberry	88:97	arg1	preservation					99:110	strawberry preservation	88:110	strawberry preservation	88:110	Preparation and characterization of Konjac glucomannan and pullulan composite films for strawberry preservation.
32532393	4	1	theme	mechanical	650:659	arg1	properties					673:682	The mechanical and barrier properties	646:682	properties	673:682	The mechanical and barrier properties of blend films were markedly enhanced although the light transmittance of which were decreased slightly.
32532393	1	2	theme	composite	155:163	arg1	films					165:169	the new composite films	147:169	the new composite films	147:169	The present study aims to develop the new composite films by blending Konjac glucomannan (KGM) and pullulan with different ratios and concentrations.
32532393	5	3	theme	blend	868:872	arg1	film					874:877	1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film	817:877	1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film	817:877	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	3	4	theme	components	634:643	arg1	compatibility					613:625	the good compatibility	604:625	the good compatibility of the components	604:643	Fourier transform infrared and scanning electron microscopy indicated the well-dispersion of film matrix was due to the good compatibility of the components.
32532393	5	5	theme	KGM/pullulan	826:837	arg1	film					874:877	1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film	817:877	1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film	817:877	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	5	6	with	KGM/pullulan	826:837	arg1	ratio					854:858	the mass ratio	845:858	the mass ratio of 2:1	845:865	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	2	7	from	%	422:422	arg1	preservation					390:401	strawberry preservation	379:401	strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH	379:471	The structural, physical, barrier properties and morphology of the films were investigated and the practical use on strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH was evaluated.
32532393	5	8	theme	titratable	941:950	arg1	acidity					952:958	the titratable acidity	937:958	the titratable acidity	937:958	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	1	9	theme	present	117:123	arg1	study					125:129	The present study	113:129	The present study	113:129	The present study aims to develop the new composite films by blending Konjac glucomannan (KGM) and pullulan with different ratios and concentrations.
32532393	5	10	theme	synthetic	1128:1136	arg1	materials					1138:1146	synthetic materials	1128:1146	synthetic materials	1128:1146	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	5	11	theme	strawberry	998:1007	arg1	preservation					1009:1020	the strawberry preservation	994:1020	the strawberry preservation	994:1020	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	2	12	theme	physical	279:286	arg1	properties					297:306	barrier properties	289:306	barrier properties	289:306	The structural, physical, barrier properties and morphology of the films were investigated and the practical use on strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH was evaluated.
32532393	2	13	theme	±5	466:467	arg1	%					468:468	25 ± 1 °C, 55 %±5% RH	451:471	%	468:468	The structural, physical, barrier properties and morphology of the films were investigated and the practical use on strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH was evaluated.
32532393	4	14	theme	barrier	665:671	arg1	properties					673:682	The mechanical and barrier properties	646:682	properties	673:682	The mechanical and barrier properties of blend films were markedly enhanced although the light transmittance of which were decreased slightly.
32532393	4	15	theme	light	735:739	arg1	transmittance					741:753	the light transmittance	731:753	the light transmittance of which	731:762	The mechanical and barrier properties of blend films were markedly enhanced although the light transmittance of which were decreased slightly.
32532393	5	16	theme	%	818:818	arg1	KGM/pullulan					826:837	1% (w/v) KGM/pullulan	817:837	1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film	817:877	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	2	17	theme	%	465:465	arg1	%					468:468	25 ± 1 °C, 55 %±5% RH	451:471	%	468:468	The structural, physical, barrier properties and morphology of the films were investigated and the practical use on strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH was evaluated.
32532393	2	18	theme	structural	267:276	arg1	properties					297:306	barrier properties	289:306	barrier properties	289:306	The structural, physical, barrier properties and morphology of the films were investigated and the practical use on strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH was evaluated.
32532393	2	19	theme	strawberry	379:388	arg1	preservation					390:401	strawberry preservation	379:401	strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH	379:471	The structural, physical, barrier properties and morphology of the films were investigated and the practical use on strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH was evaluated.
32532393	2	20	theme	relative	424:431	arg1	RH					443:444	RH	443:444	RH	443:444	The structural, physical, barrier properties and morphology of the films were investigated and the practical use on strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH was evaluated.
32532393	2	20	theme	relative	424:431	arg1	humidity					433:440	relative humidity	424:440	4 ± 1 °C, 85 %±5% relative humidity (RH)	406:445	The structural, physical, barrier properties and morphology of the films were investigated and the practical use on strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH was evaluated.
32532393	5	21	dep	proof	806:810	arg1	decrease					885:892	decrease	885:892	decrease the weight loss significantly	885:922	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	5	21	dep	proof	806:810	arg1	maintain					928:935	maintain	928:935	maintain the titratable acidity, soluble solids and skin color on the strawberry preservation	928:1020	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	5	22	from	solids	969:974	arg1	preservation					1009:1020	the strawberry preservation	994:1020	the strawberry preservation	994:1020	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	4	23	theme	films	693:697	arg1	properties					673:682	The mechanical and barrier properties	646:682	properties	673:682	The mechanical and barrier properties of blend films were markedly enhanced although the light transmittance of which were decreased slightly.
32532393	1	24	theme	Konjac	183:188	arg1	KGM					203:205	KGM	203:205	KGM	203:205	The present study aims to develop the new composite films by blending Konjac glucomannan (KGM) and pullulan with different ratios and concentrations.
32532393	1	24	theme	Konjac	183:188	arg1	glucomannan					190:200	Konjac glucomannan	183:200	Konjac glucomannan (KGM)	183:206	The present study aims to develop the new composite films by blending Konjac glucomannan (KGM) and pullulan with different ratios and concentrations.
32532393	2	25	dep	%	422:422	arg1	RH					443:444	RH	443:444	RH	443:444	The structural, physical, barrier properties and morphology of the films were investigated and the practical use on strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH was evaluated.
32532393	2	25	dep	%	422:422	arg1	humidity					433:440	relative humidity	424:440	4 ± 1 °C, 85 %±5% relative humidity (RH)	406:445	The structural, physical, barrier properties and morphology of the films were investigated and the practical use on strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH was evaluated.
32532393	3	26	theme	scanning	519:526	arg1	microscopy					537:546	scanning electron microscopy	519:546	scanning electron microscopy	519:546	Fourier transform infrared and scanning electron microscopy indicated the well-dispersion of film matrix was due to the good compatibility of the components.
32532393	2	27	theme	±5	420:421	arg1	%					422:422	4 ± 1 °C, 85 %±5%	406:422	%	422:422	The structural, physical, barrier properties and morphology of the films were investigated and the practical use on strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH was evaluated.
32532393	4	28	theme	blend	687:691	arg1	films					693:697	blend films	687:697	blend films	687:697	The mechanical and barrier properties of blend films were markedly enhanced although the light transmittance of which were decreased slightly.
32532393	5	29	from	acidity	952:958	arg1	preservation					1009:1020	the strawberry preservation	994:1020	the strawberry preservation	994:1020	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	2	30	theme	%	419:419	arg1	%					422:422	4 ± 1 °C, 85 %±5%	406:422	%	422:422	The structural, physical, barrier properties and morphology of the films were investigated and the practical use on strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH was evaluated.
32532393	5	31	theme	2:1	863:865	arg1	ratio					854:858	the mass ratio	845:858	the mass ratio of 2:1	845:865	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	5	32	theme	skin	980:983	arg1	color					985:989	skin color	980:989	skin color	980:989	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	2	33	theme	barrier	289:295	arg1	properties					297:306	barrier properties	289:306	barrier properties	289:306	The structural, physical, barrier properties and morphology of the films were investigated and the practical use on strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH was evaluated.
32532393	3	34	dep	transform	496:504	arg1	infrared					506:513	infrared	506:513	transform infrared	496:513	Fourier transform infrared and scanning electron microscopy indicated the well-dispersion of film matrix was due to the good compatibility of the components.
32532393	0	35	theme	glucomannan	43:53	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of Konjac glucomannan and pullulan composite films for strawberry preservation.
32532393	0	35	theme	glucomannan	43:53	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of Konjac glucomannan and pullulan composite films for strawberry preservation.
32532393	5	36	theme	potential	1103:1111	arg1	alternative					1113:1123	a potential alternative	1101:1123	a potential alternative to synthetic materials	1101:1146	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	5	37	dep	%	818:818	arg1	w/v					821:823	w/v	821:823	w/v	821:823	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	5	38	theme	further	798:804	arg1	proof					806:810	a further proof	796:810	a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials	796:1146	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	5	38	theme	further	798:804	arg1	It					789:790	It	789:790	It	789:790	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	0	39	theme	Konjac	36:41	arg1	glucomannan					43:53	Konjac glucomannan	36:53	Konjac glucomannan	36:53	Preparation and characterization of Konjac glucomannan and pullulan composite films for strawberry preservation.
32532393	5	40	theme	strawberries	1055:1066	arg1	qualities					1042:1050	the qualities	1038:1050	the qualities of strawberries	1038:1066	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	5	41	from	color	985:989	arg1	preservation					1009:1020	the strawberry preservation	994:1020	the strawberry preservation	994:1020	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	0	42	theme	pullulan	59:66	arg1	films					78:82	pullulan composite films	59:82	pullulan composite films	59:82	Preparation and characterization of Konjac glucomannan and pullulan composite films for strawberry preservation.
32532393	3	43	theme	electron	528:535	arg1	microscopy					537:546	scanning electron microscopy	519:546	scanning electron microscopy	519:546	Fourier transform infrared and scanning electron microscopy indicated the well-dispersion of film matrix was due to the good compatibility of the components.
32532393	2	44	from	use	372:374	arg1	preservation					390:401	strawberry preservation	379:401	strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH	379:471	The structural, physical, barrier properties and morphology of the films were investigated and the practical use on strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH was evaluated.
32532393	2	45	dep	properties	297:306	arg1	The					263:265	The	263:265	The	263:265	The structural, physical, barrier properties and morphology of the films were investigated and the practical use on strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH was evaluated.
32532393	5	46	theme	storage	1075:1081	arg1	time					1083:1086	storage time	1075:1086	storage time	1075:1086	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	5	47	theme	soluble	961:967	arg1	solids					969:974	soluble solids	961:974	soluble solids	961:974	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	2	48	theme	films	330:334	arg1	properties					297:306	barrier properties	289:306	barrier properties	289:306	The structural, physical, barrier properties and morphology of the films were investigated and the practical use on strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH was evaluated.
32532393	2	48	theme	films	330:334	arg1	morphology					312:321	morphology	312:321	morphology	312:321	The structural, physical, barrier properties and morphology of the films were investigated and the practical use on strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH was evaluated.
32532393	5	49	theme	weight	898:903	arg1	loss					905:908	the weight loss	894:908	the weight loss	894:908	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	3	50	theme	film	581:584	arg1	matrix					586:591	film matrix	581:591	film matrix	581:591	Fourier transform infrared and scanning electron microscopy indicated the well-dispersion of film matrix was due to the good compatibility of the components.
32532393	5	51	theme	mass	849:852	arg1	ratio					854:858	the mass ratio	845:858	the mass ratio of 2:1	845:865	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	3	52	theme	good	608:611	arg1	compatibility					613:625	the good compatibility	604:625	the good compatibility of the components	604:643	Fourier transform infrared and scanning electron microscopy indicated the well-dispersion of film matrix was due to the good compatibility of the components.
32532393	3	53	theme	matrix	586:591	arg1	due					597:599	due	597:599	due	597:599	Fourier transform infrared and scanning electron microscopy indicated the well-dispersion of film matrix was due to the good compatibility of the components.
32532393	3	53	theme	matrix	586:591	arg1	well-dispersion					562:576	the well-dispersion	558:576	the well-dispersion of film matrix	558:591	Fourier transform infrared and scanning electron microscopy indicated the well-dispersion of film matrix was due to the good compatibility of the components.
32532393	3	54	dep	Fourier	488:494	arg1	transform					496:504	transform	496:504	transform infrared	496:513	Fourier transform infrared and scanning electron microscopy indicated the well-dispersion of film matrix was due to the good compatibility of the components.
32532393	0	55	theme	films	78:82	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of Konjac glucomannan and pullulan composite films for strawberry preservation.
32532393	0	55	theme	films	78:82	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of Konjac glucomannan and pullulan composite films for strawberry preservation.
32532393	2	56	from	%	468:468	arg1	preservation					390:401	strawberry preservation	379:401	strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH	379:471	The structural, physical, barrier properties and morphology of the films were investigated and the practical use on strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH was evaluated.
32532393	2	57	theme	practical	362:370	arg1	use					372:374	the practical use	358:374	the practical use on strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH	358:471	The structural, physical, barrier properties and morphology of the films were investigated and the practical use on strawberry preservation at 4 ± 1 °C, 85 %±5% relative humidity (RH) and 25 ± 1 °C, 55 %±5% RH was evaluated.
32532393	5	58	theme	1	817:817	arg1	%					818:818	%	818:818	%	818:818	It was a further proof that 1% (w/v) KGM/pullulan (with the mass ratio of 2:1) blend film could decrease the weight loss significantly and maintain the titratable acidity, soluble solids and skin color on the strawberry preservation, thus improving the qualities of strawberries during storage time and offering a potential alternative to synthetic materials.
32532393	1	59	theme	different	226:234	arg1	ratios					236:241	different ratios	226:241	different ratios	226:241	The present study aims to develop the new composite films by blending Konjac glucomannan (KGM) and pullulan with different ratios and concentrations.
32532393	0	60	theme	composite	68:76	arg1	films					78:82	pullulan composite films	59:82	pullulan composite films	59:82	Preparation and characterization of Konjac glucomannan and pullulan composite films for strawberry preservation.
32532393	4	61	theme	which	758:762	arg1	transmittance					741:753	the light transmittance	731:753	the light transmittance of which	731:762	The mechanical and barrier properties of blend films were markedly enhanced although the light transmittance of which were decreased slightly.
32532393	1	62	theme	new	151:153	arg1	films					165:169	the new composite films	147:169	the new composite films	147:169	The present study aims to develop the new composite films by blending Konjac glucomannan (KGM) and pullulan with different ratios and concentrations.
34443447	1	0	theme	massive	153:159	arg1	production					161:170	the massive production	149:170	the massive production	149:170	Okara is a soybean transformation agri-food by-product, the massive production of which currently poses severe disposal issues.
34443447	0	1	theme	Plant	75:79	arg1	Protection					81:90	Plant Protection	75:90	Plant Protection	75:90	Valorization of Okara by Enzymatic Production of Anti-Fungal Compounds for Plant Protection.
34443447	7	2	theme	Antifungal	922:931	arg1	activity					933:940	Antifungal activity	922:940	Antifungal activity of the protein digested for 24 h with pancreatin against Fusarium and R. solani mycelial growth and Pseudomonas spp	922:1056	Antifungal activity of the protein digested for 24 h with pancreatin against Fusarium and R. solani mycelial growth and Pseudomonas spp was assessed.
34443447	9	3	theme	bioactive	1219:1227	arg1	peptides					1229:1236	antifungal bioactive peptides	1208:1236	antifungal bioactive peptides	1208:1236	The exploitation of okara to produce antifungal bioactive peptides has the potential to turn this by-product into a paradigmatic example of circular economy, since a field-derived food waste is transformed into a source of valuable compounds to be used in field crops protection.
34443447	9	4	contain	has	1238:1240	arg2	potential					1246:1254	the potential to turn this by-product into a paradigmatic example of circular economy	1242:1326	the potential to turn this by-product into a paradigmatic example of circular economy	1242:1326	The exploitation of okara to produce antifungal bioactive peptides has the potential to turn this by-product into a paradigmatic example of circular economy, since a field-derived food waste is transformed into a source of valuable compounds to be used in field crops protection.
34443447	9	4	contain	has	1238:1240	arg1	exploitation					1175:1186	The exploitation	1171:1186	The exploitation of okara to produce antifungal bioactive peptides	1171:1236	The exploitation of okara to produce antifungal bioactive peptides has the potential to turn this by-product into a paradigmatic example of circular economy, since a field-derived food waste is transformed into a source of valuable compounds to be used in field crops protection.
34443447	3	5	theme	compounds	485:493	arg1	okara					455:459	okara	455:459	okara	455:459	Antimicrobial and antifungal proteins and peptides have been described in plant seeds; thus, okara is a valuable source of compounds, exploitable for integrated pest management.
34443447	3	5	theme	compounds	485:493	arg1	source					475:480	a valuable source	464:480	a valuable source of compounds, exploitable for integrated pest management	464:537	Antimicrobial and antifungal proteins and peptides have been described in plant seeds; thus, okara is a valuable source of compounds, exploitable for integrated pest management.
34443447	3	6	theme	integrated	512:521	arg1	management					528:537	integrated pest management	512:537	integrated pest management	512:537	Antimicrobial and antifungal proteins and peptides have been described in plant seeds; thus, okara is a valuable source of compounds, exploitable for integrated pest management.
34443447	4	7	theme	active	688:693	arg1	product					679:685	an enzymatic proteolyzed product	654:685	an enzymatic proteolyzed product	654:685	The aim of this work is to describe a rapid and economic procedure to isolate proteins from okara, and to produce an enzymatic proteolyzed product, active against fungal plant pathogens.
34443447	4	8	theme	plant	710:714	arg1	pathogens					716:724	fungal plant pathogens	703:724	fungal plant pathogens	703:724	The aim of this work is to describe a rapid and economic procedure to isolate proteins from okara, and to produce an enzymatic proteolyzed product, active against fungal plant pathogens.
34443447	3	9	theme	pest	523:526	arg1	management					528:537	integrated pest management	512:537	integrated pest management	512:537	Antimicrobial and antifungal proteins and peptides have been described in plant seeds; thus, okara is a valuable source of compounds, exploitable for integrated pest management.
34443447	4	10	theme	work	556:559	arg1	aim					544:546	The aim	540:546	The aim of this work	540:559	The aim of this work is to describe a rapid and economic procedure to isolate proteins from okara, and to produce an enzymatic proteolyzed product, active against fungal plant pathogens.
34443447	2	11	theme	interesting	320:330	arg1	source					332:337	an interesting source	317:337	an interesting source of bioactive peptides	317:359	However, its composition is rich in seed storage proteins, which, once extracted, can represent an interesting source of bioactive peptides.
34443447	5	12	theme	%	787:787	arg1	recovery					767:774	recovery	767:774	recovery	767:774	The procedure allowed the isolation and recovery of about 30% of okara total proteins.
34443447	5	12	theme	%	787:787	arg1	isolation					753:761	isolation	753:761	isolation	753:761	The procedure allowed the isolation and recovery of about 30% of okara total proteins.
34443447	3	13	theme	exploitable	496:506	arg1	compounds					485:493	compounds	485:493	compounds	485:493	Antimicrobial and antifungal proteins and peptides have been described in plant seeds; thus, okara is a valuable source of compounds, exploitable for integrated pest management.
34443447	5	14	theme	total	798:802	arg1	proteins					804:811	okara total proteins	792:811	okara total proteins	792:811	The procedure allowed the isolation and recovery of about 30% of okara total proteins.
34443447	9	15	theme	valuable	1394:1401	arg1	compounds					1403:1411	valuable compounds	1394:1411	valuable compounds	1394:1411	The exploitation of okara to produce antifungal bioactive peptides has the potential to turn this by-product into a paradigmatic example of circular economy, since a field-derived food waste is transformed into a source of valuable compounds to be used in field crops protection.
34443447	9	16	theme	paradigmatic	1287:1298	arg1	example					1300:1306	a paradigmatic example	1285:1306	a paradigmatic example of circular economy	1285:1326	The exploitation of okara to produce antifungal bioactive peptides has the potential to turn this by-product into a paradigmatic example of circular economy, since a field-derived food waste is transformed into a source of valuable compounds to be used in field crops protection.
34443447	2	17	theme	storage	262:268	arg1	proteins					270:277	seed storage proteins	257:277	seed storage proteins	257:277	However, its composition is rich in seed storage proteins, which, once extracted, can represent an interesting source of bioactive peptides.
34443447	3	18	theme	plant	436:440	arg1	seeds					442:446	plant seeds	436:446	plant seeds	436:446	Antimicrobial and antifungal proteins and peptides have been described in plant seeds; thus, okara is a valuable source of compounds, exploitable for integrated pest management.
34443447	8	19	theme	Fusarium	1155:1162	arg1	genus					1164:1168	the Fusarium genus	1151:1168	the Fusarium genus	1151:1168	A dose-response inhibitory activity was established against fungi belonging to the Fusarium genus.
34443447	6	20	theme	proteolytic	822:832	arg1	enzymes					834:840	Several proteolytic enzymes	814:840	Several proteolytic enzymes	814:840	Several proteolytic enzymes were screened to identify the proper procedure to produce antifungal compounds.
34443447	2	21	dep	represent	307:315	arg1	proteins					270:277	seed storage proteins	257:277	seed storage proteins	257:277	However, its composition is rich in seed storage proteins, which, once extracted, can represent an interesting source of bioactive peptides.
34443447	9	22	theme	field	1427:1431	arg1	protection					1439:1448	field crops protection	1427:1448	field crops protection	1427:1448	The exploitation of okara to produce antifungal bioactive peptides has the potential to turn this by-product into a paradigmatic example of circular economy, since a field-derived food waste is transformed into a source of valuable compounds to be used in field crops protection.
34443447	3	23	theme	antifungal	380:389	arg1	proteins					391:398	proteins	391:398	proteins	391:398	Antimicrobial and antifungal proteins and peptides have been described in plant seeds; thus, okara is a valuable source of compounds, exploitable for integrated pest management.
34443447	4	24	theme	proteolyzed	667:677	arg1	product					679:685	an enzymatic proteolyzed product	654:685	an enzymatic proteolyzed product	654:685	The aim of this work is to describe a rapid and economic procedure to isolate proteins from okara, and to produce an enzymatic proteolyzed product, active against fungal plant pathogens.
34443447	2	25	from	rich	249:252	arg1	proteins					270:277	seed storage proteins	257:277	seed storage proteins	257:277	However, its composition is rich in seed storage proteins, which, once extracted, can represent an interesting source of bioactive peptides.
34443447	9	26	theme	circular	1311:1318	arg1	economy					1320:1326	circular economy	1311:1326	circular economy	1311:1326	The exploitation of okara to produce antifungal bioactive peptides has the potential to turn this by-product into a paradigmatic example of circular economy, since a field-derived food waste is transformed into a source of valuable compounds to be used in field crops protection.
34443447	7	27	theme	mycelial	1022:1029	arg1	growth					1031:1036	mycelial growth	1022:1036	mycelial growth	1022:1036	Antifungal activity of the protein digested for 24 h with pancreatin against Fusarium and R. solani mycelial growth and Pseudomonas spp was assessed.
34443447	9	28	theme	okara	1191:1195	arg1	exploitation					1175:1186	The exploitation	1171:1186	The exploitation of okara to produce antifungal bioactive peptides	1171:1236	The exploitation of okara to produce antifungal bioactive peptides has the potential to turn this by-product into a paradigmatic example of circular economy, since a field-derived food waste is transformed into a source of valuable compounds to be used in field crops protection.
34443447	4	29	theme	fungal	703:708	arg1	pathogens					716:724	fungal plant pathogens	703:724	fungal plant pathogens	703:724	The aim of this work is to describe a rapid and economic procedure to isolate proteins from okara, and to produce an enzymatic proteolyzed product, active against fungal plant pathogens.
34443447	1	30	theme	severe	197:202	arg1	issues					213:218	severe disposal issues	197:218	severe disposal issues	197:218	Okara is a soybean transformation agri-food by-product, the massive production of which currently poses severe disposal issues.
34443447	0	31	theme	Okara	16:20	arg1	Valorization					0:11	Valorization	0:11	Valorization of Okara by Enzymatic Production of Anti-Fungal Compounds for Plant Protection.	0:91	Valorization of Okara by Enzymatic Production of Anti-Fungal Compounds for Plant Protection.
34443447	9	32	theme	economy	1320:1326	arg1	example					1300:1306	a paradigmatic example	1285:1306	a paradigmatic example of circular economy	1285:1326	The exploitation of okara to produce antifungal bioactive peptides has the potential to turn this by-product into a paradigmatic example of circular economy, since a field-derived food waste is transformed into a source of valuable compounds to be used in field crops protection.
34443447	6	33	theme	Several	814:820	arg1	enzymes					834:840	Several proteolytic enzymes	814:840	Several proteolytic enzymes	814:840	Several proteolytic enzymes were screened to identify the proper procedure to produce antifungal compounds.
34443447	1	34	theme	disposal	204:211	arg1	issues					213:218	severe disposal issues	197:218	severe disposal issues	197:218	Okara is a soybean transformation agri-food by-product, the massive production of which currently poses severe disposal issues.
34443447	3	35	theme	valuable	466:473	arg1	okara					455:459	okara	455:459	okara	455:459	Antimicrobial and antifungal proteins and peptides have been described in plant seeds; thus, okara is a valuable source of compounds, exploitable for integrated pest management.
34443447	3	35	theme	valuable	466:473	arg1	source					475:480	a valuable source	464:480	a valuable source of compounds, exploitable for integrated pest management	464:537	Antimicrobial and antifungal proteins and peptides have been described in plant seeds; thus, okara is a valuable source of compounds, exploitable for integrated pest management.
34443447	9	36	theme	crops	1433:1437	arg1	protection					1439:1448	field crops protection	1427:1448	field crops protection	1427:1448	The exploitation of okara to produce antifungal bioactive peptides has the potential to turn this by-product into a paradigmatic example of circular economy, since a field-derived food waste is transformed into a source of valuable compounds to be used in field crops protection.
34443447	0	37	theme	Enzymatic	25:33	arg1	Production					35:44	Enzymatic Production	25:44	Enzymatic Production of Anti-Fungal Compounds for Plant Protection	25:90	Valorization of Okara by Enzymatic Production of Anti-Fungal Compounds for Plant Protection.
34443447	6	38	theme	antifungal	900:909	arg1	compounds					911:919	antifungal compounds	900:919	antifungal compounds	900:919	Several proteolytic enzymes were screened to identify the proper procedure to produce antifungal compounds.
34443447	9	39	theme	antifungal	1208:1217	arg1	peptides					1229:1236	antifungal bioactive peptides	1208:1236	antifungal bioactive peptides	1208:1236	The exploitation of okara to produce antifungal bioactive peptides has the potential to turn this by-product into a paradigmatic example of circular economy, since a field-derived food waste is transformed into a source of valuable compounds to be used in field crops protection.
34443447	4	40	dep	procedure	597:605	arg1	produce					646:652	produce	646:652	to produce an enzymatic proteolyzed product, active against fungal plant pathogens	643:724	The aim of this work is to describe a rapid and economic procedure to isolate proteins from okara, and to produce an enzymatic proteolyzed product, active against fungal plant pathogens.
34443447	4	40	dep	procedure	597:605	arg1	isolate					610:616	isolate	610:616	to isolate proteins from okara	607:636	The aim of this work is to describe a rapid and economic procedure to isolate proteins from okara, and to produce an enzymatic proteolyzed product, active against fungal plant pathogens.
34443447	9	41	link	field-derived	1337:1349	arg1	waste					1356:1360	a field-derived food waste	1335:1360	a field-derived food waste	1335:1360	The exploitation of okara to produce antifungal bioactive peptides has the potential to turn this by-product into a paradigmatic example of circular economy, since a field-derived food waste is transformed into a source of valuable compounds to be used in field crops protection.
34443447	5	42	theme	okara	792:796	arg1	proteins					804:811	okara total proteins	792:811	okara total proteins	792:811	The procedure allowed the isolation and recovery of about 30% of okara total proteins.
34443447	0	43	theme	Anti-Fungal	49:59	arg1	Compounds					61:69	Anti-Fungal Compounds	49:69	Anti-Fungal Compounds	49:69	Valorization of Okara by Enzymatic Production of Anti-Fungal Compounds for Plant Protection.
34443447	7	44	dep	solani	1015:1020	arg1	growth					1031:1036	mycelial growth	1022:1036	mycelial growth	1022:1036	Antifungal activity of the protein digested for 24 h with pancreatin against Fusarium and R. solani mycelial growth and Pseudomonas spp was assessed.
34443447	8	45	theme	dose-response	1074:1086	arg1	activity					1099:1106	A dose-response inhibitory activity	1072:1106	A dose-response inhibitory activity	1072:1106	A dose-response inhibitory activity was established against fungi belonging to the Fusarium genus.
34443447	1	46	theme	soybean	104:110	arg1	Okara					93:97	Okara	93:97	Okara	93:97	Okara is a soybean transformation agri-food by-product, the massive production of which currently poses severe disposal issues.
34443447	1	46	theme	soybean	104:110	arg1	by-product					137:146	a soybean transformation agri-food by-product	102:146	a soybean transformation agri-food by-product	102:146	Okara is a soybean transformation agri-food by-product, the massive production of which currently poses severe disposal issues.
34443447	9	47	theme	field-derived	1337:1349	arg1	waste					1356:1360	a field-derived food waste	1335:1360	a field-derived food waste	1335:1360	The exploitation of okara to produce antifungal bioactive peptides has the potential to turn this by-product into a paradigmatic example of circular economy, since a field-derived food waste is transformed into a source of valuable compounds to be used in field crops protection.
34443447	9	48	theme	compounds	1403:1411	arg1	source					1384:1389	a source	1382:1389	a source of valuable compounds	1382:1411	The exploitation of okara to produce antifungal bioactive peptides has the potential to turn this by-product into a paradigmatic example of circular economy, since a field-derived food waste is transformed into a source of valuable compounds to be used in field crops protection.
34443447	1	49	theme	transformation	112:125	arg1	Okara					93:97	Okara	93:97	Okara	93:97	Okara is a soybean transformation agri-food by-product, the massive production of which currently poses severe disposal issues.
34443447	1	49	theme	transformation	112:125	arg1	by-product					137:146	a soybean transformation agri-food by-product	102:146	a soybean transformation agri-food by-product	102:146	Okara is a soybean transformation agri-food by-product, the massive production of which currently poses severe disposal issues.
34443447	2	50	from	proteins	270:277	arg1	rich					249:252	rich	249:252	rich	249:252	However, its composition is rich in seed storage proteins, which, once extracted, can represent an interesting source of bioactive peptides.
34443447	9	51	theme	food	1351:1354	arg1	waste					1356:1360	a field-derived food waste	1335:1360	a field-derived food waste	1335:1360	The exploitation of okara to produce antifungal bioactive peptides has the potential to turn this by-product into a paradigmatic example of circular economy, since a field-derived food waste is transformed into a source of valuable compounds to be used in field crops protection.
34443447	4	52	theme	enzymatic	657:665	arg1	product					679:685	an enzymatic proteolyzed product	654:685	an enzymatic proteolyzed product	654:685	The aim of this work is to describe a rapid and economic procedure to isolate proteins from okara, and to produce an enzymatic proteolyzed product, active against fungal plant pathogens.
34443447	2	53	theme	seed	257:260	arg1	proteins					270:277	seed storage proteins	257:277	seed storage proteins	257:277	However, its composition is rich in seed storage proteins, which, once extracted, can represent an interesting source of bioactive peptides.
34443447	1	54	theme	agri-food	127:135	arg1	Okara					93:97	Okara	93:97	Okara	93:97	Okara is a soybean transformation agri-food by-product, the massive production of which currently poses severe disposal issues.
34443447	1	54	theme	agri-food	127:135	arg1	by-product					137:146	a soybean transformation agri-food by-product	102:146	a soybean transformation agri-food by-product	102:146	Okara is a soybean transformation agri-food by-product, the massive production of which currently poses severe disposal issues.
34443447	7	55	theme	protein	949:955	arg1	activity					933:940	Antifungal activity	922:940	Antifungal activity of the protein digested for 24 h with pancreatin against Fusarium and R. solani mycelial growth and Pseudomonas spp	922:1056	Antifungal activity of the protein digested for 24 h with pancreatin against Fusarium and R. solani mycelial growth and Pseudomonas spp was assessed.
34443447	1	56	theme	by-product	137:146	arg1	production					161:170	the massive production	149:170	the massive production	149:170	Okara is a soybean transformation agri-food by-product, the massive production of which currently poses severe disposal issues.
34443447	8	57	theme	inhibitory	1088:1097	arg1	activity					1099:1106	A dose-response inhibitory activity	1072:1106	A dose-response inhibitory activity	1072:1106	A dose-response inhibitory activity was established against fungi belonging to the Fusarium genus.
34443447	4	58	theme	economic	588:595	arg1	procedure					597:605	a rapid and economic procedure	576:605	a rapid and economic procedure to isolate proteins from okara, and to produce an enzymatic proteolyzed product, active against fungal plant pathogens	576:724	The aim of this work is to describe a rapid and economic procedure to isolate proteins from okara, and to produce an enzymatic proteolyzed product, active against fungal plant pathogens.
34443447	2	59	theme	peptides	352:359	arg1	source					332:337	an interesting source	317:337	an interesting source of bioactive peptides	317:359	However, its composition is rich in seed storage proteins, which, once extracted, can represent an interesting source of bioactive peptides.
34443447	5	60	theme	proteins	804:811	arg1	proteins					804:811	okara total proteins	792:811	okara total proteins	792:811	The procedure allowed the isolation and recovery of about 30% of okara total proteins.
34443447	5	60	theme	proteins	804:811	arg1	%					787:787	about 30%	779:787	about 30% of okara total proteins	779:811	The procedure allowed the isolation and recovery of about 30% of okara total proteins.
34443447	4	61	theme	rapid	578:582	arg1	procedure					597:605	a rapid and economic procedure	576:605	a rapid and economic procedure to isolate proteins from okara, and to produce an enzymatic proteolyzed product, active against fungal plant pathogens	576:724	The aim of this work is to describe a rapid and economic procedure to isolate proteins from okara, and to produce an enzymatic proteolyzed product, active against fungal plant pathogens.
34443447	0	62	theme	Compounds	61:69	arg1	Production					35:44	Enzymatic Production	25:44	Enzymatic Production of Anti-Fungal Compounds for Plant Protection	25:90	Valorization of Okara by Enzymatic Production of Anti-Fungal Compounds for Plant Protection.
34443447	6	63	theme	proper	872:877	arg1	procedure					879:887	the proper procedure	868:887	the proper procedure to produce antifungal compounds	868:919	Several proteolytic enzymes were screened to identify the proper procedure to produce antifungal compounds.
34443447	3	64	theme	Antimicrobial	362:374	arg1	proteins					391:398	proteins	391:398	proteins	391:398	Antimicrobial and antifungal proteins and peptides have been described in plant seeds; thus, okara is a valuable source of compounds, exploitable for integrated pest management.
34443447	2	65	theme	bioactive	342:350	arg1	peptides					352:359	bioactive peptides	342:359	bioactive peptides	342:359	However, its composition is rich in seed storage proteins, which, once extracted, can represent an interesting source of bioactive peptides.
34443447	9	66	used	used	1419:1422	arg2	waste					1356:1360	a field-derived food waste	1335:1360	a field-derived food waste	1335:1360	The exploitation of okara to produce antifungal bioactive peptides has the potential to turn this by-product into a paradigmatic example of circular economy, since a field-derived food waste is transformed into a source of valuable compounds to be used in field crops protection.
32172856	0	0	theme	tunable	104:110	arg1	properties					112:121	tunable properties	104:121	tunable properties	104:121	Extracellular matrix-mimetic composite hydrogels of cross-linked hyaluronan and fibrillar collagen with tunable properties and ultrastructure.
32172856	1	1	theme	biological	395:404	arg1	properties					406:415	their physical, chemical and biological properties	366:415	their physical, chemical and biological properties	366:415	A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties.
32172856	7	2	theme	tissue	1220:1225	arg1	applications					1239:1250	novel tissue engineering applications	1214:1250	novel tissue engineering applications following a biomimetic approach	1214:1282	Those Col/HA-Tyr hydrogels appear promising for novel tissue engineering applications following a biomimetic approach.
32172856	3	3	theme	Col	661:663	arg1	fibrils					665:671	the banded Col fibrils	650:671	the banded Col fibrils	650:671	At high HA-Tyr and Horse Radish Peroxydase (HRP) content, a microfibrillar network was formed beside the banded Col fibrils and a synergistic effect of the hybrid structure on mechanical properties was observed.
32172856	1	4	theme	derivative	212:221	arg1	hydrogels					236:244	enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels	157:244	enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions	157:294	A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties.
32172856	3	5	theme	mechanical	725:734	arg1	properties					736:745	mechanical properties	725:745	mechanical properties	725:745	At high HA-Tyr and Horse Radish Peroxydase (HRP) content, a microfibrillar network was formed beside the banded Col fibrils and a synergistic effect of the hybrid structure on mechanical properties was observed.
32172856	7	6	theme	engineering	1227:1237	arg1	applications					1239:1250	novel tissue engineering applications	1214:1250	novel tissue engineering applications following a biomimetic approach	1214:1282	Those Col/HA-Tyr hydrogels appear promising for novel tissue engineering applications following a biomimetic approach.
32172856	3	7	theme	Horse	568:572	arg1	content					598:604	Horse Radish Peroxydase (HRP) content	568:604	Horse Radish Peroxydase (HRP) content	568:604	At high HA-Tyr and Horse Radish Peroxydase (HRP) content, a microfibrillar network was formed beside the banded Col fibrils and a synergistic effect of the hybrid structure on mechanical properties was observed.
32172856	6	8	theme	HRP	1041:1043	arg1	concentrations					1045:1058	high HA-Tyr and HRP concentrations	1025:1058	high HA-Tyr and HRP concentrations	1025:1058	These results showed that high HA-Tyr and HRP concentrations are required to positively impact the physical properties of hydrogels while preserving collagen fibrils.
32172856	7	9	theme	novel	1214:1218	arg1	applications					1239:1250	novel tissue engineering applications	1214:1250	novel tissue engineering applications following a biomimetic approach	1214:1282	Those Col/HA-Tyr hydrogels appear promising for novel tissue engineering applications following a biomimetic approach.
32172856	2	10	theme	low	421:423	arg1	content					432:438	low HA-Tyr content	421:438	low HA-Tyr content	421:438	At low HA-Tyr content, hydrogels exhibited a fibrillar structure, with lower mechanical properties compared to pure Col hydrogels.
32172856	5	11	theme	dermal	919:924	arg1	fibroblasts					926:936	human dermal fibroblasts	913:936	human dermal fibroblasts	913:936	Unlike HA-Tyr hydrogels, encapsulation of human dermal fibroblasts within Col/HA-Tyr hydrogels allowed for high cell viability.
32172856	3	12	from	effect	691:696	arg1	properties					736:745	mechanical properties	725:745	mechanical properties	725:745	At high HA-Tyr and Horse Radish Peroxydase (HRP) content, a microfibrillar network was formed beside the banded Col fibrils and a synergistic effect of the hybrid structure on mechanical properties was observed.
32172856	5	13	theme	cell	983:986	arg1	viability					988:996	high cell viability	978:996	high cell viability	978:996	Unlike HA-Tyr hydrogels, encapsulation of human dermal fibroblasts within Col/HA-Tyr hydrogels allowed for high cell viability.
32172856	1	14	theme	Col/HA-Tyr	224:233	arg1	hydrogels					236:244	enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels	157:244	enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions	157:294	A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties.
32172856	4	15	theme	hydration	860:868	arg1	degree					850:855	a high degree	843:855	a high degree of hydration	843:868	These hydrogels were highly resistant against enzymatic degradation while keeping a high degree of hydration.
32172856	3	16	theme	HRP	593:595	arg1	content					598:604	Horse Radish Peroxydase (HRP) content	568:604	Horse Radish Peroxydase (HRP) content	568:604	At high HA-Tyr and Horse Radish Peroxydase (HRP) content, a microfibrillar network was formed beside the banded Col fibrils and a synergistic effect of the hybrid structure on mechanical properties was observed.
32172856	5	17	theme	fibroblasts	926:936	arg1	encapsulation					896:908	encapsulation	896:908	encapsulation of human dermal fibroblasts within Col/HA-Tyr hydrogels	896:964	Unlike HA-Tyr hydrogels, encapsulation of human dermal fibroblasts within Col/HA-Tyr hydrogels allowed for high cell viability.
32172856	0	18	theme	matrix-mimetic	14:27	arg1	hydrogels					39:47	Extracellular matrix-mimetic composite hydrogels	0:47	Extracellular matrix-mimetic composite hydrogels of cross-linked hyaluronan and fibrillar collagen with tunable properties and ultrastructure.	0:141	Extracellular matrix-mimetic composite hydrogels of cross-linked hyaluronan and fibrillar collagen with tunable properties and ultrastructure.
32172856	3	19	theme	Peroxydase	581:590	arg1	content					598:604	Horse Radish Peroxydase (HRP) content	568:604	Horse Radish Peroxydase (HRP) content	568:604	At high HA-Tyr and Horse Radish Peroxydase (HRP) content, a microfibrillar network was formed beside the banded Col fibrils and a synergistic effect of the hybrid structure on mechanical properties was observed.
32172856	7	20	theme	Col/HA-Tyr	1172:1181	arg1	hydrogels					1183:1191	Those Col/HA-Tyr hydrogels	1166:1191	Those Col/HA-Tyr hydrogels	1166:1191	Those Col/HA-Tyr hydrogels appear promising for novel tissue engineering applications following a biomimetic approach.
32172856	3	21	theme	microfibrillar	609:622	arg1	network					624:630	a microfibrillar network	607:630	a microfibrillar network	607:630	At high HA-Tyr and Horse Radish Peroxydase (HRP) content, a microfibrillar network was formed beside the banded Col fibrils and a synergistic effect of the hybrid structure on mechanical properties was observed.
32172856	1	22	theme	hydrogels	236:244	arg1	platform					145:152	A platform	143:152	A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions	143:294	A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties.
32172856	0	23	theme	Extracellular	0:12	arg1	hydrogels					39:47	Extracellular matrix-mimetic composite hydrogels	0:47	Extracellular matrix-mimetic composite hydrogels of cross-linked hyaluronan and fibrillar collagen with tunable properties and ultrastructure.	0:141	Extracellular matrix-mimetic composite hydrogels of cross-linked hyaluronan and fibrillar collagen with tunable properties and ultrastructure.
32172856	3	24	theme	hybrid	705:710	arg1	structure					712:720	the hybrid structure	701:720	the hybrid structure	701:720	At high HA-Tyr and Horse Radish Peroxydase (HRP) content, a microfibrillar network was formed beside the banded Col fibrils and a synergistic effect of the hybrid structure on mechanical properties was observed.
32172856	0	25	link	cross-linked	52:63	arg1	hyaluronan					65:74	cross-linked hyaluronan	52:74	cross-linked hyaluronan	52:74	Extracellular matrix-mimetic composite hydrogels of cross-linked hyaluronan and fibrillar collagen with tunable properties and ultrastructure.
32172856	5	26	theme	Col/HA-Tyr	945:954	arg1	hydrogels					956:964	Col/HA-Tyr hydrogels	945:964	Col/HA-Tyr hydrogels	945:964	Unlike HA-Tyr hydrogels, encapsulation of human dermal fibroblasts within Col/HA-Tyr hydrogels allowed for high cell viability.
32172856	2	27	theme	mechanical	495:504	arg1	properties					506:515	lower mechanical properties	489:515	lower mechanical properties	489:515	At low HA-Tyr content, hydrogels exhibited a fibrillar structure, with lower mechanical properties compared to pure Col hydrogels.
32172856	1	28	theme	preparation	340:350	arg1	conditions					352:361	the preparation conditions	336:361	the preparation conditions	336:361	A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties.
32172856	1	29	from	impact	326:331	arg1	properties					406:415	their physical, chemical and biological properties	366:415	their physical, chemical and biological properties	366:415	A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties.
32172856	4	30	theme	enzymatic	807:815	arg1	degradation					817:827	enzymatic degradation	807:827	enzymatic degradation	807:827	These hydrogels were highly resistant against enzymatic degradation while keeping a high degree of hydration.
32172856	1	31	theme	tunable	251:257	arg1	compositions					259:270	tunable compositions	251:270	tunable compositions	251:270	A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties.
32172856	0	32	theme	composite	29:37	arg1	hydrogels					39:47	Extracellular matrix-mimetic composite hydrogels	0:47	Extracellular matrix-mimetic composite hydrogels of cross-linked hyaluronan and fibrillar collagen with tunable properties and ultrastructure.	0:141	Extracellular matrix-mimetic composite hydrogels of cross-linked hyaluronan and fibrillar collagen with tunable properties and ultrastructure.
32172856	1	33	theme	conditions	352:361	arg1	impact					326:331	the impact	322:331	the impact of the preparation conditions on their physical, chemical and biological properties	322:415	A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties.
32172856	5	34	theme	high	978:981	arg1	viability					988:996	high cell viability	978:996	high cell viability	978:996	Unlike HA-Tyr hydrogels, encapsulation of human dermal fibroblasts within Col/HA-Tyr hydrogels allowed for high cell viability.
32172856	3	35	theme	Radish	574:579	arg1	content					598:604	Horse Radish Peroxydase (HRP) content	568:604	Horse Radish Peroxydase (HRP) content	568:604	At high HA-Tyr and Horse Radish Peroxydase (HRP) content, a microfibrillar network was formed beside the banded Col fibrils and a synergistic effect of the hybrid structure on mechanical properties was observed.
32172856	0	36	theme	cross-linked	52:63	arg1	hyaluronan					65:74	cross-linked hyaluronan	52:74	cross-linked hyaluronan	52:74	Extracellular matrix-mimetic composite hydrogels of cross-linked hyaluronan and fibrillar collagen with tunable properties and ultrastructure.
32172856	6	37	theme	HA-Tyr	1030:1035	arg1	concentrations					1045:1058	high HA-Tyr and HRP concentrations	1025:1058	high HA-Tyr and HRP concentrations	1025:1058	These results showed that high HA-Tyr and HRP concentrations are required to positively impact the physical properties of hydrogels while preserving collagen fibrils.
32172856	5	38	theme	human	913:917	arg1	fibroblasts					926:936	human dermal fibroblasts	913:936	human dermal fibroblasts	913:936	Unlike HA-Tyr hydrogels, encapsulation of human dermal fibroblasts within Col/HA-Tyr hydrogels allowed for high cell viability.
32172856	6	39	theme	hydrogels	1121:1129	arg1	properties					1107:1116	the physical properties	1094:1116	the physical properties of hydrogels	1094:1129	These results showed that high HA-Tyr and HRP concentrations are required to positively impact the physical properties of hydrogels while preserving collagen fibrils.
32172856	6	40	theme	collagen	1148:1155	arg1	fibrils					1157:1163	collagen fibrils	1148:1163	collagen fibrils	1148:1163	These results showed that high HA-Tyr and HRP concentrations are required to positively impact the physical properties of hydrogels while preserving collagen fibrils.
32172856	3	41	dep	fibrils	665:671	arg1	beside					643:648	beside	643:648	beside	643:648	At high HA-Tyr and Horse Radish Peroxydase (HRP) content, a microfibrillar network was formed beside the banded Col fibrils and a synergistic effect of the hybrid structure on mechanical properties was observed.
32172856	2	42	theme	Col	534:536	arg1	hydrogels					538:546	pure Col hydrogels	529:546	pure Col hydrogels	529:546	At low HA-Tyr content, hydrogels exhibited a fibrillar structure, with lower mechanical properties compared to pure Col hydrogels.
32172856	6	43	theme	high	1025:1028	arg1	concentrations					1045:1058	high HA-Tyr and HRP concentrations	1025:1058	high HA-Tyr and HRP concentrations	1025:1058	These results showed that high HA-Tyr and HRP concentrations are required to positively impact the physical properties of hydrogels while preserving collagen fibrils.
32172856	7	44	theme	biomimetic	1264:1273	arg1	approach					1275:1282	a biomimetic approach	1262:1282	a biomimetic approach	1262:1282	Those Col/HA-Tyr hydrogels appear promising for novel tissue engineering applications following a biomimetic approach.
32172856	5	45	theme	HA-Tyr	878:883	arg1	hydrogels					885:893	HA-Tyr hydrogels	878:893	HA-Tyr hydrogels	878:893	Unlike HA-Tyr hydrogels, encapsulation of human dermal fibroblasts within Col/HA-Tyr hydrogels allowed for high cell viability.
32172856	0	46	with	hydrogels	39:47	arg1	ultrastructure					127:140	ultrastructure	127:140	ultrastructure	127:140	Extracellular matrix-mimetic composite hydrogels of cross-linked hyaluronan and fibrillar collagen with tunable properties and ultrastructure.
32172856	0	46	with	hydrogels	39:47	arg1	properties					112:121	tunable properties	104:121	tunable properties	104:121	Extracellular matrix-mimetic composite hydrogels of cross-linked hyaluronan and fibrillar collagen with tunable properties and ultrastructure.
32172856	1	47	theme	gelation	276:283	arg1	conditions					285:294	gelation conditions	276:294	gelation conditions	276:294	A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties.
32172856	2	48	theme	pure	529:532	arg1	hydrogels					538:546	pure Col hydrogels	529:546	pure Col hydrogels	529:546	At low HA-Tyr content, hydrogels exhibited a fibrillar structure, with lower mechanical properties compared to pure Col hydrogels.
32172856	1	49	theme	physical	372:379	arg1	properties					406:415	their physical, chemical and biological properties	366:415	their physical, chemical and biological properties	366:415	A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties.
32172856	1	50	with	hydrogels	236:244	arg1	compositions					259:270	tunable compositions	251:270	tunable compositions	251:270	A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties.
32172856	1	50	with	hydrogels	236:244	arg1	conditions					285:294	gelation conditions	276:294	gelation conditions	276:294	A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties.
32172856	3	51	theme	synergistic	679:689	arg1	effect					691:696	a synergistic effect	677:696	a synergistic effect of the hybrid structure on mechanical properties	677:745	At high HA-Tyr and Horse Radish Peroxydase (HRP) content, a microfibrillar network was formed beside the banded Col fibrils and a synergistic effect of the hybrid structure on mechanical properties was observed.
32172856	3	52	theme	high	552:555	arg1	HA-Tyr					557:562	high HA-Tyr	552:562	high HA-Tyr	552:562	At high HA-Tyr and Horse Radish Peroxydase (HRP) content, a microfibrillar network was formed beside the banded Col fibrils and a synergistic effect of the hybrid structure on mechanical properties was observed.
32172856	0	53	theme	hyaluronan	65:74	arg1	hydrogels					39:47	Extracellular matrix-mimetic composite hydrogels	0:47	Extracellular matrix-mimetic composite hydrogels of cross-linked hyaluronan and fibrillar collagen with tunable properties and ultrastructure.	0:141	Extracellular matrix-mimetic composite hydrogels of cross-linked hyaluronan and fibrillar collagen with tunable properties and ultrastructure.
32172856	1	54	link	enzymatically-crosslinked	157:181	arg1	hydrogels					236:244	enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels	157:244	enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions	157:294	A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties.
32172856	3	55	theme	structure	712:720	arg1	effect					691:696	a synergistic effect	677:696	a synergistic effect of the hybrid structure on mechanical properties	677:745	At high HA-Tyr and Horse Radish Peroxydase (HRP) content, a microfibrillar network was formed beside the banded Col fibrils and a synergistic effect of the hybrid structure on mechanical properties was observed.
32172856	2	56	theme	HA-Tyr	425:430	arg1	content					432:438	low HA-Tyr content	421:438	low HA-Tyr content	421:438	At low HA-Tyr content, hydrogels exhibited a fibrillar structure, with lower mechanical properties compared to pure Col hydrogels.
32172856	1	57	theme	chemical	382:389	arg1	properties					406:415	their physical, chemical and biological properties	366:415	their physical, chemical and biological properties	366:415	A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties.
32172856	4	58	theme	high	845:848	arg1	degree					850:855	a high degree	843:855	a high degree of hydration	843:868	These hydrogels were highly resistant against enzymatic degradation while keeping a high degree of hydration.
32172856	1	59	theme	enzymatically-crosslinked	157:181	arg1	hydrogels					236:244	enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels	157:244	enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions	157:294	A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties.
32172856	6	60	theme	physical	1098:1105	arg1	properties					1107:1116	the physical properties	1094:1116	the physical properties of hydrogels	1094:1129	These results showed that high HA-Tyr and HRP concentrations are required to positively impact the physical properties of hydrogels while preserving collagen fibrils.
32172856	0	61	theme	collagen	90:97	arg1	hydrogels					39:47	Extracellular matrix-mimetic composite hydrogels	0:47	Extracellular matrix-mimetic composite hydrogels of cross-linked hyaluronan and fibrillar collagen with tunable properties and ultrastructure.	0:141	Extracellular matrix-mimetic composite hydrogels of cross-linked hyaluronan and fibrillar collagen with tunable properties and ultrastructure.
32172856	2	62	theme	fibrillar	463:471	arg1	structure					473:481	a fibrillar structure	461:481	a fibrillar structure	461:481	At low HA-Tyr content, hydrogels exhibited a fibrillar structure, with lower mechanical properties compared to pure Col hydrogels.
32172856	3	63	theme	banded	654:659	arg1	fibrils					665:671	the banded Col fibrils	650:671	the banded Col fibrils	650:671	At high HA-Tyr and Horse Radish Peroxydase (HRP) content, a microfibrillar network was formed beside the banded Col fibrils and a synergistic effect of the hybrid structure on mechanical properties was observed.
32172856	1	64	theme	Collagen/Tyramine	183:199	arg1	hydrogels					236:244	enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels	157:244	enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions	157:294	A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties.
32172856	0	65	theme	fibrillar	80:88	arg1	collagen					90:97	fibrillar collagen	80:97	fibrillar collagen	80:97	Extracellular matrix-mimetic composite hydrogels of cross-linked hyaluronan and fibrillar collagen with tunable properties and ultrastructure.
32172856	2	66	theme	lower	489:493	arg1	properties					506:515	lower mechanical properties	489:515	lower mechanical properties	489:515	At low HA-Tyr content, hydrogels exhibited a fibrillar structure, with lower mechanical properties compared to pure Col hydrogels.
32172856	1	67	theme	hyaluronan	201:210	arg1	hydrogels					236:244	enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels	157:244	enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions	157:294	A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties.
33436256	0	0	theme	barley	78:83	arg1	starch					85:90	barley starch	78:90	barley starch without amylopectin isolation	78:120	A simplified method of determining the internal structure of amylopectin from barley starch without amylopectin isolation.
33436256	6	1	theme	building	956:963	arg1	blocks					965:970	more large building blocks	945:970	more large building blocks	945:970	The starch from amo1-mutated varieties had more large building blocks than that from waxy varieties.
33436256	2	2	theme	extensive	345:353	arg1	α-amylase					355:363	extensive α-amylase	345:363	extensive α-amylase	345:363	The β-LDs were treated with extensive α-amylase to prepare α-limit dextrins (α-LDs), and the α-LDs were further hydrolyzed with β-amylase into building blocks.
33436256	5	3	theme	barley	771:776	arg1	varieties					791:799	barley amo1-mutated varieties	771:799	barley amo1-mutated varieties	771:799	The starch of barley amo1-mutated varieties contained more short internal B-chains and less long internal B-chains than that of other varieties.
33436256	2	4	theme	building	460:467	arg1	blocks					469:474	building blocks	460:474	building blocks	460:474	The β-LDs were treated with extensive α-amylase to prepare α-limit dextrins (α-LDs), and the α-LDs were further hydrolyzed with β-amylase into building blocks.
33436256	6	5	theme	large	950:954	arg1	blocks					965:970	more large building blocks	945:970	more large building blocks	945:970	The starch from amo1-mutated varieties had more large building blocks than that from waxy varieties.
33436256	7	6	theme	amylopectin	1183:1193	arg1	structure					1195:1203	amylopectin structure	1183:1203	amylopectin structure	1183:1203	The simplified method presented in this study can effectively characterize starch internal structure that relates to physicochemical properties of starch, although some details of amylopectin structure are not assessable.
33436256	1	7	theme	barley	162:167	arg1	starch					169:174	barley starch	162:174	barley starch	162:174	To determine the internal structure of barley starch without amylopectin isolation, whole starch was hydrolyzed using β-amylase to remove the linear amylose and obtain β-limit dextrins (β-LDs).
33436256	5	8	dep	less	844:847	arg1	B-chains					863:870	long internal B-chains	849:870	long internal B-chains	849:870	The starch of barley amo1-mutated varieties contained more short internal B-chains and less long internal B-chains than that of other varieties.
33436256	7	9	theme	simplified	1007:1016	arg1	method					1018:1023	The simplified method	1003:1023	The simplified method presented in this study	1003:1047	The simplified method presented in this study can effectively characterize starch internal structure that relates to physicochemical properties of starch, although some details of amylopectin structure are not assessable.
33436256	3	10	theme	block	528:532	arg1	composition					534:544	β-LD and building block composition	510:544	β-LD and building block composition	510:544	The chain-length distribution of β-LD and building block composition were analyzed by size-exclusion chromatography and anion-exchange chromatography.
33436256	1	11	theme	linear	265:270	arg1	amylose					272:278	the linear amylose	261:278	the linear amylose	261:278	To determine the internal structure of barley starch without amylopectin isolation, whole starch was hydrolyzed using β-amylase to remove the linear amylose and obtain β-limit dextrins (β-LDs).
33436256	3	12	theme	composition	534:544	arg1	distribution					494:505	The chain-length distribution	477:505	The chain-length distribution of β-LD and building block composition	477:544	The chain-length distribution of β-LD and building block composition were analyzed by size-exclusion chromatography and anion-exchange chromatography.
33436256	3	13	theme	chain-length	481:492	arg1	distribution					494:505	The chain-length distribution	477:505	The chain-length distribution of β-LD and building block composition	477:544	The chain-length distribution of β-LD and building block composition were analyzed by size-exclusion chromatography and anion-exchange chromatography.
33436256	2	14	theme	α-limit	376:382	arg1	dextrins					384:391	α-limit dextrins	376:391	α-limit dextrins (α-LDs)	376:399	The β-LDs were treated with extensive α-amylase to prepare α-limit dextrins (α-LDs), and the α-LDs were further hydrolyzed with β-amylase into building blocks.
33436256	2	14	theme	α-limit	376:382	arg1	α-LDs					394:398	α-LDs	394:398	α-LDs	394:398	The β-LDs were treated with extensive α-amylase to prepare α-limit dextrins (α-LDs), and the α-LDs were further hydrolyzed with β-amylase into building blocks.
33436256	1	15	theme	starch	169:174	arg1	structure					149:157	the internal structure	136:157	the internal structure of barley starch	136:174	To determine the internal structure of barley starch without amylopectin isolation, whole starch was hydrolyzed using β-amylase to remove the linear amylose and obtain β-limit dextrins (β-LDs).
33436256	0	16	theme	amylopectin	100:110	arg1	isolation					112:120	amylopectin isolation	100:120	amylopectin isolation	100:120	A simplified method of determining the internal structure of amylopectin from barley starch without amylopectin isolation.
33436256	6	17	theme	amo1-mutated	918:929	arg1	varieties					931:939	amo1-mutated varieties	918:939	amo1-mutated varieties	918:939	The starch from amo1-mutated varieties had more large building blocks than that from waxy varieties.
33436256	6	18	from	varieties	931:939	arg1	starch					906:911	The starch	902:911	The starch from amo1-mutated varieties	902:939	The starch from amo1-mutated varieties had more large building blocks than that from waxy varieties.
33436256	3	19	theme	building	519:526	arg1	composition					534:544	β-LD and building block composition	510:544	β-LD and building block composition	510:544	The chain-length distribution of β-LD and building block composition were analyzed by size-exclusion chromatography and anion-exchange chromatography.
33436256	4	20	theme	starches	671:678	arg1	structure					641:649	The internal structure	628:649	The internal structure of the barley whole starches	628:678	The internal structure of the barley whole starches had similar pattern to barley amylopectins analyzed by conventional methods.
33436256	0	21	theme	simplified	2:11	arg1	method					13:18	A simplified method	0:18	A simplified method of determining the internal structure of amylopectin from barley starch without amylopectin isolation.	0:121	A simplified method of determining the internal structure of amylopectin from barley starch without amylopectin isolation.
33436256	5	22	theme	amo1-mutated	778:789	arg1	varieties					791:799	barley amo1-mutated varieties	771:799	barley amo1-mutated varieties	771:799	The starch of barley amo1-mutated varieties contained more short internal B-chains and less long internal B-chains than that of other varieties.
33436256	1	23	theme	amylopectin	184:194	arg1	isolation					196:204	amylopectin isolation	184:204	amylopectin isolation	184:204	To determine the internal structure of barley starch without amylopectin isolation, whole starch was hydrolyzed using β-amylase to remove the linear amylose and obtain β-limit dextrins (β-LDs).
33436256	5	24	theme	long	849:852	arg1	B-chains					863:870	long internal B-chains	849:870	long internal B-chains	849:870	The starch of barley amo1-mutated varieties contained more short internal B-chains and less long internal B-chains than that of other varieties.
33436256	4	25	contain	had	680:682	arg2	pattern					692:698	similar pattern	684:698	similar pattern	684:698	The internal structure of the barley whole starches had similar pattern to barley amylopectins analyzed by conventional methods.
33436256	4	25	contain	had	680:682	arg1	structure					641:649	The internal structure	628:649	The internal structure of the barley whole starches	628:678	The internal structure of the barley whole starches had similar pattern to barley amylopectins analyzed by conventional methods.
33436256	3	26	theme	size-exclusion	563:576	arg1	chromatography					578:591	size-exclusion chromatography	563:591	size-exclusion chromatography	563:591	The chain-length distribution of β-LD and building block composition were analyzed by size-exclusion chromatography and anion-exchange chromatography.
33436256	4	27	theme	similar	684:690	arg1	pattern					692:698	similar pattern	684:698	similar pattern	684:698	The internal structure of the barley whole starches had similar pattern to barley amylopectins analyzed by conventional methods.
33436256	5	28	theme	internal	854:861	arg1	B-chains					863:870	long internal B-chains	849:870	long internal B-chains	849:870	The starch of barley amo1-mutated varieties contained more short internal B-chains and less long internal B-chains than that of other varieties.
33436256	7	29	theme	physicochemical	1120:1134	arg1	properties					1136:1145	physicochemical properties	1120:1145	physicochemical properties of starch	1120:1155	The simplified method presented in this study can effectively characterize starch internal structure that relates to physicochemical properties of starch, although some details of amylopectin structure are not assessable.
33436256	1	30	theme	β-limit	291:297	arg1	β-LDs					309:313	β-LDs	309:313	β-LDs	309:313	To determine the internal structure of barley starch without amylopectin isolation, whole starch was hydrolyzed using β-amylase to remove the linear amylose and obtain β-limit dextrins (β-LDs).
33436256	1	30	theme	β-limit	291:297	arg1	dextrins					299:306	β-limit dextrins	291:306	β-limit dextrins (β-LDs)	291:314	To determine the internal structure of barley starch without amylopectin isolation, whole starch was hydrolyzed using β-amylase to remove the linear amylose and obtain β-limit dextrins (β-LDs).
33436256	5	31	dep	more	811:814	arg1	B-chains					831:838	short internal B-chains	816:838	short internal B-chains	816:838	The starch of barley amo1-mutated varieties contained more short internal B-chains and less long internal B-chains than that of other varieties.
33436256	1	32	theme	whole	207:211	arg1	starch					213:218	whole starch	207:218	whole starch	207:218	To determine the internal structure of barley starch without amylopectin isolation, whole starch was hydrolyzed using β-amylase to remove the linear amylose and obtain β-limit dextrins (β-LDs).
33436256	5	33	theme	short	816:820	arg1	B-chains					831:838	short internal B-chains	816:838	short internal B-chains	816:838	The starch of barley amo1-mutated varieties contained more short internal B-chains and less long internal B-chains than that of other varieties.
33436256	3	34	theme	anion-exchange	597:610	arg1	chromatography					612:625	anion-exchange chromatography	597:625	anion-exchange chromatography	597:625	The chain-length distribution of β-LD and building block composition were analyzed by size-exclusion chromatography and anion-exchange chromatography.
33436256	4	35	theme	whole	665:669	arg1	starches					671:678	the barley whole starches	654:678	the barley whole starches	654:678	The internal structure of the barley whole starches had similar pattern to barley amylopectins analyzed by conventional methods.
33436256	4	36	theme	barley	703:708	arg1	amylopectins					710:721	barley amylopectins	703:721	barley amylopectins analyzed by conventional methods	703:754	The internal structure of the barley whole starches had similar pattern to barley amylopectins analyzed by conventional methods.
33436256	6	37	theme	waxy	987:990	arg1	varieties					992:1000	waxy varieties	987:1000	waxy varieties	987:1000	The starch from amo1-mutated varieties had more large building blocks than that from waxy varieties.
33436256	5	38	theme	internal	822:829	arg1	B-chains					831:838	short internal B-chains	816:838	short internal B-chains	816:838	The starch of barley amo1-mutated varieties contained more short internal B-chains and less long internal B-chains than that of other varieties.
33436256	5	39	theme	other	885:889	arg1	varieties					891:899	other varieties	885:899	other varieties	885:899	The starch of barley amo1-mutated varieties contained more short internal B-chains and less long internal B-chains than that of other varieties.
33436256	4	40	theme	barley	658:663	arg1	starches					671:678	the barley whole starches	654:678	the barley whole starches	654:678	The internal structure of the barley whole starches had similar pattern to barley amylopectins analyzed by conventional methods.
33436256	7	41	theme	structure	1195:1203	arg1	details					1172:1178	some details	1167:1178	some details of amylopectin structure	1167:1203	The simplified method presented in this study can effectively characterize starch internal structure that relates to physicochemical properties of starch, although some details of amylopectin structure are not assessable.
33436256	4	42	theme	conventional	735:746	arg1	methods					748:754	conventional methods	735:754	conventional methods	735:754	The internal structure of the barley whole starches had similar pattern to barley amylopectins analyzed by conventional methods.
33436256	0	43	theme	internal	39:46	arg1	structure					48:56	the internal structure	35:56	the internal structure of amylopectin from barley starch without amylopectin isolation	35:120	A simplified method of determining the internal structure of amylopectin from barley starch without amylopectin isolation.
33436256	7	44	theme	starch	1078:1083	arg1	structure					1094:1102	starch internal structure	1078:1102	starch internal structure that relates to physicochemical properties of starch, although some details of amylopectin structure are not assessable	1078:1222	The simplified method presented in this study can effectively characterize starch internal structure that relates to physicochemical properties of starch, although some details of amylopectin structure are not assessable.
33436256	6	45	contain	had	941:943	arg2	blocks					965:970	more large building blocks	945:970	more large building blocks	945:970	The starch from amo1-mutated varieties had more large building blocks than that from waxy varieties.
33436256	6	45	contain	had	941:943	arg1	starch					906:911	The starch	902:911	The starch from amo1-mutated varieties	902:939	The starch from amo1-mutated varieties had more large building blocks than that from waxy varieties.
33436256	7	46	theme	internal	1085:1092	arg1	structure					1094:1102	starch internal structure	1078:1102	starch internal structure that relates to physicochemical properties of starch, although some details of amylopectin structure are not assessable	1078:1222	The simplified method presented in this study can effectively characterize starch internal structure that relates to physicochemical properties of starch, although some details of amylopectin structure are not assessable.
33436256	7	47	theme	starch	1150:1155	arg1	properties					1136:1145	physicochemical properties	1120:1145	physicochemical properties of starch	1120:1155	The simplified method presented in this study can effectively characterize starch internal structure that relates to physicochemical properties of starch, although some details of amylopectin structure are not assessable.
33436256	0	48	theme	amylopectin	61:71	arg1	structure					48:56	the internal structure	35:56	the internal structure of amylopectin from barley starch without amylopectin isolation	35:120	A simplified method of determining the internal structure of amylopectin from barley starch without amylopectin isolation.
33436256	0	49	from	starch	85:90	arg1	structure					48:56	the internal structure	35:56	the internal structure of amylopectin from barley starch without amylopectin isolation	35:120	A simplified method of determining the internal structure of amylopectin from barley starch without amylopectin isolation.
33436256	1	50	theme	internal	140:147	arg1	structure					149:157	the internal structure	136:157	the internal structure of barley starch	136:174	To determine the internal structure of barley starch without amylopectin isolation, whole starch was hydrolyzed using β-amylase to remove the linear amylose and obtain β-limit dextrins (β-LDs).
33436256	5	51	theme	varieties	791:799	arg1	starch					761:766	The starch	757:766	The starch of barley amo1-mutated varieties	757:799	The starch of barley amo1-mutated varieties contained more short internal B-chains and less long internal B-chains than that of other varieties.
33436256	3	52	theme	β-LD	510:513	arg1	composition					534:544	β-LD and building block composition	510:544	β-LD and building block composition	510:544	The chain-length distribution of β-LD and building block composition were analyzed by size-exclusion chromatography and anion-exchange chromatography.
33436256	4	53	theme	internal	632:639	arg1	structure					641:649	The internal structure	628:649	The internal structure of the barley whole starches	628:678	The internal structure of the barley whole starches had similar pattern to barley amylopectins analyzed by conventional methods.
33152853	3	0	theme	<1.0	438:441	arg1	KG					434:435	KG	434:435	KG	434:435	The SEM showed that KG (<1.0%) reduced the appearance of moisture channels and promoted the formation of an integral MP gel network.
33152853	3	0	theme	<1.0	438:441	arg1	%					442:442	<1.0%	438:442	<1.0%	438:442	The SEM showed that KG (<1.0%) reduced the appearance of moisture channels and promoted the formation of an integral MP gel network.
33152853	0	1	theme	konjac	69:74	arg1	glucomannan					76:86	konjac glucomannan	69:86	konjac glucomannan	69:86	Insight into the mechanism of myofibrillar protein gel influenced by konjac glucomannan: Moisture stability and phase separation behavior.
33152853	0	2	theme	Moisture	89:96	arg1	stability					98:106	Moisture stability	89:106	Moisture stability	89:106	Insight into the mechanism of myofibrillar protein gel influenced by konjac glucomannan: Moisture stability and phase separation behavior.
33152853	2	3	theme	KG	267:268	arg1	addition					270:277	The KG addition	263:277	The KG addition	263:277	The KG addition significantly improved gel strength and water holding capability (WHC) of MP-KG composite gel, but it had additive limitation at 1.0%.
33152853	4	4	theme	hydrophobic	652:662	arg1	groups					664:669	hydrophobic groups	652:669	hydrophobic groups	652:669	Raman spectroscopy showed that KG addition (<1.0%) promoted the protein unfolding and the interaction of hydrophobic groups during thermal processing.
33152853	6	5	theme	separation	1139:1148	arg1	behavior					1150:1157	phase separation behavior	1133:1157	phase separation behavior	1133:1157	Hence, MP gels produced with the addition of KG underwent a transformation from a loose structure to a compact structure to an unaggregated structure, which was influenced by moisture stability and phase separation behavior.
33152853	3	6	theme	moisture	471:478	arg1	channels					480:487	moisture channels	471:487	moisture channels	471:487	The SEM showed that KG (<1.0%) reduced the appearance of moisture channels and promoted the formation of an integral MP gel network.
33152853	6	7	theme	phase	1133:1137	arg1	behavior					1150:1157	phase separation behavior	1133:1157	phase separation behavior	1133:1157	Hence, MP gels produced with the addition of KG underwent a transformation from a loose structure to a compact structure to an unaggregated structure, which was influenced by moisture stability and phase separation behavior.
33152853	6	8	theme	compact	1038:1044	arg1	structure					1046:1054	a compact structure	1036:1054	a compact structure to an unaggregated structure, which was influenced by moisture stability and phase separation behavior	1036:1157	Hence, MP gels produced with the addition of KG underwent a transformation from a loose structure to a compact structure to an unaggregated structure, which was influenced by moisture stability and phase separation behavior.
33152853	2	9	theme	MP-KG	353:357	arg1	gel					369:371	MP-KG composite gel	353:371	MP-KG composite gel	353:371	The KG addition significantly improved gel strength and water holding capability (WHC) of MP-KG composite gel, but it had additive limitation at 1.0%.
33152853	2	10	theme	gel	369:371	arg1	strength					306:313	gel strength	302:313	gel strength	302:313	The KG addition significantly improved gel strength and water holding capability (WHC) of MP-KG composite gel, but it had additive limitation at 1.0%.
33152853	2	10	theme	gel	369:371	arg1	WHC					345:347	WHC	345:347	WHC	345:347	The KG addition significantly improved gel strength and water holding capability (WHC) of MP-KG composite gel, but it had additive limitation at 1.0%.
33152853	2	10	theme	gel	369:371	arg1	capability					333:342	water holding capability	319:342	water holding capability (WHC) of MP-KG composite gel	319:371	The KG addition significantly improved gel strength and water holding capability (WHC) of MP-KG composite gel, but it had additive limitation at 1.0%.
33152853	6	11	theme	loose	1017:1021	arg1	structure					1023:1031	a loose structure	1015:1031	a loose structure	1015:1031	Hence, MP gels produced with the addition of KG underwent a transformation from a loose structure to a compact structure to an unaggregated structure, which was influenced by moisture stability and phase separation behavior.
33152853	1	12	theme	myofibrillar	167:178	arg1	protein					180:186	myofibrillar protein	167:186	myofibrillar protein (MP) gelation influenced by konjac glucomannan (KG) addition	167:247	The effect and mechanism of myofibrillar protein (MP) gelation influenced by konjac glucomannan (KG) addition were studied.
33152853	1	12	theme	myofibrillar	167:178	arg1	MP					189:190	MP	189:190	MP	189:190	The effect and mechanism of myofibrillar protein (MP) gelation influenced by konjac glucomannan (KG) addition were studied.
33152853	4	13	theme	KG	578:579	arg1	addition					581:588	KG addition	578:588	KG addition (<1.0%)	578:596	Raman spectroscopy showed that KG addition (<1.0%) promoted the protein unfolding and the interaction of hydrophobic groups during thermal processing.
33152853	4	13	theme	KG	578:579	arg1	%					595:595	<1.0%	591:595	<1.0%	591:595	Raman spectroscopy showed that KG addition (<1.0%) promoted the protein unfolding and the interaction of hydrophobic groups during thermal processing.
33152853	2	14	theme	gel	302:304	arg1	strength					306:313	gel strength	302:313	gel strength	302:313	The KG addition significantly improved gel strength and water holding capability (WHC) of MP-KG composite gel, but it had additive limitation at 1.0%.
33152853	1	15	theme	protein	180:186	arg1	gelation					193:200	myofibrillar protein (MP) gelation	167:200	myofibrillar protein (MP) gelation influenced by konjac glucomannan (KG) addition	167:247	The effect and mechanism of myofibrillar protein (MP) gelation influenced by konjac glucomannan (KG) addition were studied.
33152853	6	16	theme	moisture	1110:1117	arg1	stability					1119:1127	moisture stability	1110:1127	moisture stability	1110:1127	Hence, MP gels produced with the addition of KG underwent a transformation from a loose structure to a compact structure to an unaggregated structure, which was influenced by moisture stability and phase separation behavior.
33152853	5	17	theme	hydrophobic	836:846	arg1	groups					848:853	hydrophobic groups	836:853	hydrophobic groups	836:853	However, the KG (>1.0%) would form continuous viscous hydrogel and interpenetrate with the MP solution, which hindered the interaction of hydrophobic groups during thermal process, and the MP formed a loose and degraded final structure.
33152853	6	18	theme	MP	942:943	arg1	gels					945:948	MP gels	942:948	MP gels produced with the addition of KG	942:981	Hence, MP gels produced with the addition of KG underwent a transformation from a loose structure to a compact structure to an unaggregated structure, which was influenced by moisture stability and phase separation behavior.
33152853	6	19	theme	unaggregated	1062:1073	arg1	structure					1075:1083	an unaggregated structure	1059:1083	an unaggregated structure	1059:1083	Hence, MP gels produced with the addition of KG underwent a transformation from a loose structure to a compact structure to an unaggregated structure, which was influenced by moisture stability and phase separation behavior.
33152853	0	20	theme	separation	118:127	arg1	behavior					129:136	phase separation behavior	112:136	phase separation behavior	112:136	Insight into the mechanism of myofibrillar protein gel influenced by konjac glucomannan: Moisture stability and phase separation behavior.
33152853	3	21	theme	channels	480:487	arg1	appearance					457:466	the appearance	453:466	the appearance of moisture channels	453:487	The SEM showed that KG (<1.0%) reduced the appearance of moisture channels and promoted the formation of an integral MP gel network.
33152853	5	22	theme	thermal	862:868	arg1	process					870:876	thermal process	862:876	thermal process	862:876	However, the KG (>1.0%) would form continuous viscous hydrogel and interpenetrate with the MP solution, which hindered the interaction of hydrophobic groups during thermal process, and the MP formed a loose and degraded final structure.
33152853	2	23	theme	additive	385:392	arg1	limitation					394:403	additive limitation	385:403	additive limitation	385:403	The KG addition significantly improved gel strength and water holding capability (WHC) of MP-KG composite gel, but it had additive limitation at 1.0%.
33152853	0	24	theme	phase	112:116	arg1	behavior					129:136	phase separation behavior	112:136	phase separation behavior	112:136	Insight into the mechanism of myofibrillar protein gel influenced by konjac glucomannan: Moisture stability and phase separation behavior.
33152853	5	25	theme	groups	848:853	arg1	interaction					821:831	the interaction	817:831	the interaction of hydrophobic groups	817:853	However, the KG (>1.0%) would form continuous viscous hydrogel and interpenetrate with the MP solution, which hindered the interaction of hydrophobic groups during thermal process, and the MP formed a loose and degraded final structure.
33152853	1	26	dep	effect	143:148	arg1	The					139:141	The	139:141	The	139:141	The effect and mechanism of myofibrillar protein (MP) gelation influenced by konjac glucomannan (KG) addition were studied.
33152853	4	27	theme	Raman	547:551	arg1	spectroscopy					553:564	Raman spectroscopy	547:564	Raman spectroscopy	547:564	Raman spectroscopy showed that KG addition (<1.0%) promoted the protein unfolding and the interaction of hydrophobic groups during thermal processing.
33152853	0	28	theme	myofibrillar	30:41	arg1	gel					51:53	myofibrillar protein gel	30:53	myofibrillar protein gel influenced by konjac glucomannan	30:86	Insight into the mechanism of myofibrillar protein gel influenced by konjac glucomannan: Moisture stability and phase separation behavior.
33152853	4	29	theme	protein	611:617	arg1	unfolding					619:627	the protein unfolding	607:627	the protein unfolding	607:627	Raman spectroscopy showed that KG addition (<1.0%) promoted the protein unfolding and the interaction of hydrophobic groups during thermal processing.
33152853	5	30	theme	continuous	733:742	arg1	hydrogel					752:759	continuous viscous hydrogel	733:759	continuous viscous hydrogel	733:759	However, the KG (>1.0%) would form continuous viscous hydrogel and interpenetrate with the MP solution, which hindered the interaction of hydrophobic groups during thermal process, and the MP formed a loose and degraded final structure.
33152853	5	31	theme	loose	899:903	arg1	structure					924:932	a loose and degraded final structure	897:932	a loose and degraded final structure	897:932	However, the KG (>1.0%) would form continuous viscous hydrogel and interpenetrate with the MP solution, which hindered the interaction of hydrophobic groups during thermal process, and the MP formed a loose and degraded final structure.
33152853	6	32	from	structure	1023:1031	arg1	transformation					995:1008	a transformation	993:1008	a transformation from a loose structure to a compact structure to an unaggregated structure, which was influenced by moisture stability and phase separation behavior	993:1157	Hence, MP gels produced with the addition of KG underwent a transformation from a loose structure to a compact structure to an unaggregated structure, which was influenced by moisture stability and phase separation behavior.
33152853	1	33	theme	gelation	193:200	arg1	effect					143:148	effect	143:148	effect	143:148	The effect and mechanism of myofibrillar protein (MP) gelation influenced by konjac glucomannan (KG) addition were studied.
33152853	1	33	theme	gelation	193:200	arg1	mechanism					154:162	mechanism	154:162	mechanism	154:162	The effect and mechanism of myofibrillar protein (MP) gelation influenced by konjac glucomannan (KG) addition were studied.
33152853	3	34	theme	integral	522:529	arg1	network					538:544	an integral MP gel network	519:544	an integral MP gel network	519:544	The SEM showed that KG (<1.0%) reduced the appearance of moisture channels and promoted the formation of an integral MP gel network.
33152853	5	35	theme	viscous	744:750	arg1	hydrogel					752:759	continuous viscous hydrogel	733:759	continuous viscous hydrogel	733:759	However, the KG (>1.0%) would form continuous viscous hydrogel and interpenetrate with the MP solution, which hindered the interaction of hydrophobic groups during thermal process, and the MP formed a loose and degraded final structure.
33152853	0	36	theme	gel	51:53	arg1	mechanism					17:25	the mechanism	13:25	the mechanism of myofibrillar protein gel influenced by konjac glucomannan	13:86	Insight into the mechanism of myofibrillar protein gel influenced by konjac glucomannan: Moisture stability and phase separation behavior.
33152853	4	37	theme	<1.0	591:594	arg1	addition					581:588	KG addition	578:588	KG addition (<1.0%)	578:596	Raman spectroscopy showed that KG addition (<1.0%) promoted the protein unfolding and the interaction of hydrophobic groups during thermal processing.
33152853	4	37	theme	<1.0	591:594	arg1	%					595:595	<1.0%	591:595	<1.0%	591:595	Raman spectroscopy showed that KG addition (<1.0%) promoted the protein unfolding and the interaction of hydrophobic groups during thermal processing.
33152853	5	38	theme	degraded	909:916	arg1	structure					924:932	a loose and degraded final structure	897:932	a loose and degraded final structure	897:932	However, the KG (>1.0%) would form continuous viscous hydrogel and interpenetrate with the MP solution, which hindered the interaction of hydrophobic groups during thermal process, and the MP formed a loose and degraded final structure.
33152853	2	39	theme	holding	325:331	arg1	WHC					345:347	WHC	345:347	WHC	345:347	The KG addition significantly improved gel strength and water holding capability (WHC) of MP-KG composite gel, but it had additive limitation at 1.0%.
33152853	2	39	theme	holding	325:331	arg1	capability					333:342	water holding capability	319:342	water holding capability (WHC) of MP-KG composite gel	319:371	The KG addition significantly improved gel strength and water holding capability (WHC) of MP-KG composite gel, but it had additive limitation at 1.0%.
33152853	0	40	theme	protein	43:49	arg1	gel					51:53	myofibrillar protein gel	30:53	myofibrillar protein gel influenced by konjac glucomannan	30:86	Insight into the mechanism of myofibrillar protein gel influenced by konjac glucomannan: Moisture stability and phase separation behavior.
33152853	5	41	theme	final	918:922	arg1	structure					924:932	a loose and degraded final structure	897:932	a loose and degraded final structure	897:932	However, the KG (>1.0%) would form continuous viscous hydrogel and interpenetrate with the MP solution, which hindered the interaction of hydrophobic groups during thermal process, and the MP formed a loose and degraded final structure.
33152853	2	42	theme	water	319:323	arg1	WHC					345:347	WHC	345:347	WHC	345:347	The KG addition significantly improved gel strength and water holding capability (WHC) of MP-KG composite gel, but it had additive limitation at 1.0%.
33152853	2	42	theme	water	319:323	arg1	capability					333:342	water holding capability	319:342	water holding capability (WHC) of MP-KG composite gel	319:371	The KG addition significantly improved gel strength and water holding capability (WHC) of MP-KG composite gel, but it had additive limitation at 1.0%.
33152853	3	43	theme	gel	534:536	arg1	network					538:544	an integral MP gel network	519:544	an integral MP gel network	519:544	The SEM showed that KG (<1.0%) reduced the appearance of moisture channels and promoted the formation of an integral MP gel network.
33152853	3	44	theme	network	538:544	arg1	formation					506:514	the formation	502:514	the formation of an integral MP gel network	502:544	The SEM showed that KG (<1.0%) reduced the appearance of moisture channels and promoted the formation of an integral MP gel network.
33152853	4	45	theme	groups	664:669	arg1	unfolding					619:627	the protein unfolding	607:627	the protein unfolding	607:627	Raman spectroscopy showed that KG addition (<1.0%) promoted the protein unfolding and the interaction of hydrophobic groups during thermal processing.
33152853	4	45	theme	groups	664:669	arg1	interaction					637:647	the interaction	633:647	the interaction of hydrophobic groups during thermal processing	633:695	Raman spectroscopy showed that KG addition (<1.0%) promoted the protein unfolding and the interaction of hydrophobic groups during thermal processing.
33152853	4	46	theme	thermal	678:684	arg1	processing					686:695	thermal processing	678:695	thermal processing	678:695	Raman spectroscopy showed that KG addition (<1.0%) promoted the protein unfolding and the interaction of hydrophobic groups during thermal processing.
33152853	1	47	theme	konjac	216:221	arg1	addition					240:247	konjac glucomannan (KG) addition	216:247	konjac glucomannan (KG) addition	216:247	The effect and mechanism of myofibrillar protein (MP) gelation influenced by konjac glucomannan (KG) addition were studied.
33152853	2	48	contain	had	381:383	arg1	it					378:379	it	378:379	it	378:379	The KG addition significantly improved gel strength and water holding capability (WHC) of MP-KG composite gel, but it had additive limitation at 1.0%.
33152853	2	48	contain	had	381:383	arg2	limitation					394:403	additive limitation	385:403	additive limitation	385:403	The KG addition significantly improved gel strength and water holding capability (WHC) of MP-KG composite gel, but it had additive limitation at 1.0%.
33152853	3	49	theme	MP	531:532	arg1	network					538:544	an integral MP gel network	519:544	an integral MP gel network	519:544	The SEM showed that KG (<1.0%) reduced the appearance of moisture channels and promoted the formation of an integral MP gel network.
33152853	6	50	theme	KG	980:981	arg1	addition					968:975	the addition	964:975	the addition of KG	964:981	Hence, MP gels produced with the addition of KG underwent a transformation from a loose structure to a compact structure to an unaggregated structure, which was influenced by moisture stability and phase separation behavior.
33152853	2	51	theme	composite	359:367	arg1	gel					369:371	MP-KG composite gel	353:371	MP-KG composite gel	353:371	The KG addition significantly improved gel strength and water holding capability (WHC) of MP-KG composite gel, but it had additive limitation at 1.0%.
33152853	1	52	theme	glucomannan	223:233	arg1	addition					240:247	konjac glucomannan (KG) addition	216:247	konjac glucomannan (KG) addition	216:247	The effect and mechanism of myofibrillar protein (MP) gelation influenced by konjac glucomannan (KG) addition were studied.
33152853	0	53	dep	Insight	0:6	arg1	behavior					129:136	phase separation behavior	112:136	phase separation behavior	112:136	Insight into the mechanism of myofibrillar protein gel influenced by konjac glucomannan: Moisture stability and phase separation behavior.
33152853	0	53	dep	Insight	0:6	arg1	stability					98:106	Moisture stability	89:106	Moisture stability	89:106	Insight into the mechanism of myofibrillar protein gel influenced by konjac glucomannan: Moisture stability and phase separation behavior.
33152853	5	54	theme	MP	789:790	arg1	solution					792:799	the MP solution	785:799	the MP solution	785:799	However, the KG (>1.0%) would form continuous viscous hydrogel and interpenetrate with the MP solution, which hindered the interaction of hydrophobic groups during thermal process, and the MP formed a loose and degraded final structure.
32653379	7	0	theme	sustained	1417:1425	arg1	profile					1435:1441	a sustained release profile	1415:1441	a sustained release profile with release similarity factor of 94.9	1415:1480	The optimized formulation showed excellent DEE of 89.2 ± 4.4% and a sustained release profile with release similarity factor of 94.9.
32653379	0	1	link	ion-cross-linked	108:123	arg1	mucilage					154:161	pectin and okra mucilage	138:161	mucilage	154:161	Quality-by-design approach for development of sustained-release multiple-unit beads of lamotrigine based on ion-cross-linked composite of pectin and okra mucilage: An in vitro appraisal.
32653379	0	1	link	ion-cross-linked	108:123	arg1	composite					125:133	ion-cross-linked composite	108:133	ion-cross-linked composite of pectin and okra mucilage	108:161	Quality-by-design approach for development of sustained-release multiple-unit beads of lamotrigine based on ion-cross-linked composite of pectin and okra mucilage: An in vitro appraisal.
32653379	1	2	theme	sustained	244:252	arg1	release					254:260	a sustained release	242:260	a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM)	242:400	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	7	3	theme	optimized	1353:1361	arg1	formulation					1363:1373	The optimized formulation	1349:1373	The optimized formulation	1349:1373	The optimized formulation showed excellent DEE of 89.2 ± 4.4% and a sustained release profile with release similarity factor of 94.9.
32653379	2	4	theme	experimental	649:660	arg1	design					662:667	the experimental design	645:667	the experimental design	645:667	Two different levels of three independent variables (total polymer concentration, polymer ratio and [CaCl2]) were considered for the experimental design.
32653379	8	5	theme	Kinetic	1483:1489	arg1	modeling					1491:1498	Kinetic modeling	1483:1498	Kinetic modeling of drug release data	1483:1519	Kinetic modeling of drug release data demonstrated a release mechanism combined of hydration, diffusion and erosion.
32653379	8	6	theme	drug	1503:1506	arg1	data					1516:1519	drug release data	1503:1519	drug release data	1503:1519	Kinetic modeling of drug release data demonstrated a release mechanism combined of hydration, diffusion and erosion.
32653379	1	7	theme	release	254:260	arg1	beads					276:280	a sustained release multiple-unit beads	242:280	a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM)	242:400	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	7	8	theme	94.9	1477:1480	arg1	factor					1467:1472	release similarity factor	1448:1472	release similarity factor of 94.9	1448:1480	The optimized formulation showed excellent DEE of 89.2 ± 4.4% and a sustained release profile with release similarity factor of 94.9.
32653379	1	9	theme	polymer	442:448	arg1	ratio					450:454	polymer ratio	442:454	polymer ratio	442:454	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	7	10	theme	release	1427:1433	arg1	profile					1435:1441	a sustained release profile	1415:1441	a sustained release profile with release similarity factor of 94.9	1415:1480	The optimized formulation showed excellent DEE of 89.2 ± 4.4% and a sustained release profile with release similarity factor of 94.9.
32653379	1	11	theme	multiple-unit	262:274	arg1	beads					276:280	a sustained release multiple-unit beads	242:280	a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM)	242:400	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	0	12	theme	ion-cross-linked	108:123	arg1	mucilage					154:161	pectin and okra mucilage	138:161	mucilage	154:161	Quality-by-design approach for development of sustained-release multiple-unit beads of lamotrigine based on ion-cross-linked composite of pectin and okra mucilage: An in vitro appraisal.
32653379	0	12	theme	ion-cross-linked	108:123	arg1	composite					125:133	ion-cross-linked composite	108:133	ion-cross-linked composite of pectin and okra mucilage	108:161	Quality-by-design approach for development of sustained-release multiple-unit beads of lamotrigine based on ion-cross-linked composite of pectin and okra mucilage: An in vitro appraisal.
32653379	7	13	theme	similarity	1456:1465	arg1	factor					1467:1472	release similarity factor	1448:1472	release similarity factor of 94.9	1448:1480	The optimized formulation showed excellent DEE of 89.2 ± 4.4% and a sustained release profile with release similarity factor of 94.9.
32653379	2	14	theme	polymer	598:604	arg1	variables					558:566	three independent variables	540:566	three independent variables (total polymer concentration, polymer ratio and [CaCl2])	540:623	Two different levels of three independent variables (total polymer concentration, polymer ratio and [CaCl2]) were considered for the experimental design.
32653379	2	14	theme	polymer	598:604	arg1	ratio					606:610	polymer ratio	598:610	polymer ratio	598:610	Two different levels of three independent variables (total polymer concentration, polymer ratio and [CaCl2]) were considered for the experimental design.
32653379	5	15	theme	difference	970:979	arg1	factors					981:987	difference factors	970:987	difference factors	970:987	Effects of the independent variables on bead-size, drug-encapsulation-efficiency (DEE), drug-release along with release similarity and difference factors were examined.
32653379	6	16	from	2 h	1258:1260	arg1	similarity					1314:1323	release similarity	1306:1323	release similarity	1306:1323	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	6	16	from	2 h	1258:1260	arg1	release					1247:1253	percent drug release	1234:1253	percent drug release at 2 h	1234:1260	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	6	16	from	2 h	1258:1260	arg1	DEE					1229:1231	DEE	1229:1231	DEE	1229:1231	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	6	16	from	2 h	1258:1260	arg1	constant					1296:1303	Korsmeyer-Peppas rate constant	1274:1303	Korsmeyer-Peppas rate constant	1274:1303	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	6	16	from	2 h	1258:1260	arg1	factors					1340:1346	difference factors	1329:1346	difference factors	1329:1346	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	6	16	from	2 h	1258:1260	arg1	5 h					1263:1265	5 h	1263:1265	5 h	1263:1265	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	6	16	from	2 h	1258:1260	arg1	12 h					1268:1271	12 h	1268:1271	12 h	1268:1271	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	0	17	theme	pectin	138:143	arg1	mucilage					154:161	pectin and okra mucilage	138:161	mucilage	154:161	Quality-by-design approach for development of sustained-release multiple-unit beads of lamotrigine based on ion-cross-linked composite of pectin and okra mucilage: An in vitro appraisal.
32653379	6	18	theme	rate	1291:1294	arg1	constant					1296:1303	Korsmeyer-Peppas rate constant	1274:1303	Korsmeyer-Peppas rate constant	1274:1303	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	4	19	theme	bead	775:778	arg1	morphology					757:766	The surface morphology	745:766	The surface morphology of the bead before and after dissolution test	745:812	The surface morphology of the bead before and after dissolution test was examined by SEM.
32653379	5	20	theme	variables	862:870	arg1	Effects					835:841	Effects	835:841	Effects of the independent variables on bead-size, drug-encapsulation-efficiency (DEE), drug-release along with release similarity and difference factors	835:987	Effects of the independent variables on bead-size, drug-encapsulation-efficiency (DEE), drug-release along with release similarity and difference factors were examined.
32653379	7	21	with	DEE	1392:1394	arg1	factor					1467:1472	release similarity factor	1448:1472	release similarity factor of 94.9	1448:1480	The optimized formulation showed excellent DEE of 89.2 ± 4.4% and a sustained release profile with release similarity factor of 94.9.
32653379	2	22	dep	variables	558:566	arg1	variables					558:566	three independent variables	540:566	three independent variables (total polymer concentration, polymer ratio and [CaCl2])	540:623	Two different levels of three independent variables (total polymer concentration, polymer ratio and [CaCl2]) were considered for the experimental design.
32653379	2	22	dep	variables	558:566	arg1	[CaCl2					616:621	[CaCl2	616:621	[CaCl2	616:621	Two different levels of three independent variables (total polymer concentration, polymer ratio and [CaCl2]) were considered for the experimental design.
32653379	2	22	dep	variables	558:566	arg1	concentration					583:595	total polymer concentration	569:595	total polymer concentration	569:595	Two different levels of three independent variables (total polymer concentration, polymer ratio and [CaCl2]) were considered for the experimental design.
32653379	2	22	dep	variables	558:566	arg1	ratio					606:610	polymer ratio	598:610	polymer ratio	598:610	Two different levels of three independent variables (total polymer concentration, polymer ratio and [CaCl2]) were considered for the experimental design.
32653379	8	23	theme	release	1536:1542	arg1	mechanism					1544:1552	a release mechanism combined	1534:1561	a release mechanism combined of hydration, diffusion and erosion	1534:1597	Kinetic modeling of drug release data demonstrated a release mechanism combined of hydration, diffusion and erosion.
32653379	6	24	theme	independent	1008:1018	arg1	variables					1020:1028	The independent variables	1004:1028	The independent variables	1004:1028	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	1	25	theme	cross-linker	460:471	arg1	concentration					473:485	cross-linker concentration	460:485	cross-linker concentration	460:485	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	6	26	theme	variance	1181:1188	arg1	analysis					1169:1176	the analysis	1165:1176	the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors	1165:1346	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	7	27	theme	release	1448:1454	arg1	factor					1467:1472	release similarity factor	1448:1472	release similarity factor of 94.9	1448:1480	The optimized formulation showed excellent DEE of 89.2 ± 4.4% and a sustained release profile with release similarity factor of 94.9.
32653379	2	28	theme	different	520:528	arg1	levels					530:535	Two different levels	516:535	Two different levels of three independent variables (total polymer concentration, polymer ratio and [CaCl2])	516:623	Two different levels of three independent variables (total polymer concentration, polymer ratio and [CaCl2]) were considered for the experimental design.
32653379	1	29	theme	okra	383:386	arg1	OM					398:399	OM	398:399	OM	398:399	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	1	29	theme	okra	383:386	arg1	mucilage					388:395	okra mucilage	383:395	okra mucilage (OM)	383:400	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	8	30	theme	erosion	1591:1597	arg1	mechanism					1544:1552	a release mechanism combined	1534:1561	a release mechanism combined of hydration, diffusion and erosion	1534:1597	Kinetic modeling of drug release data demonstrated a release mechanism combined of hydration, diffusion and erosion.
32653379	8	31	theme	combined	1554:1561	arg1	mechanism					1544:1552	a release mechanism combined	1534:1561	a release mechanism combined of hydration, diffusion and erosion	1534:1597	Kinetic modeling of drug release data demonstrated a release mechanism combined of hydration, diffusion and erosion.
32653379	6	32	theme	Korsmeyer-Peppas	1274:1289	arg1	constant					1296:1303	Korsmeyer-Peppas rate constant	1274:1303	Korsmeyer-Peppas rate constant	1274:1303	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	8	33	theme	data	1516:1519	arg1	modeling					1491:1498	Kinetic modeling	1483:1498	Kinetic modeling of drug release data	1483:1519	Kinetic modeling of drug release data demonstrated a release mechanism combined of hydration, diffusion and erosion.
32653379	1	34	theme	main	191:194	arg1	objective					196:204	The main objective	187:204	The main objective of the present study	187:225	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	0	35	theme	Quality-by-design	0:16	arg1	approach					18:25	Quality-by-design approach	0:25	Quality-by-design approach for development of sustained-release multiple-unit beads of lamotrigine based on ion-cross-linked composite of pectin and okra mucilage	0:161	Quality-by-design approach for development of sustained-release multiple-unit beads of lamotrigine based on ion-cross-linked composite of pectin and okra mucilage: An in vitro appraisal.
32653379	6	36	theme	parameters	1210:1219	arg1	variance					1181:1188	variance	1181:1188	variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors	1181:1346	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	1	37	dep	polymer-concentration	419:439	arg1	the					415:417	the	415:417	the	415:417	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	1	38	theme	lamotrigine	285:295	arg1	beads					276:280	a sustained release multiple-unit beads	242:280	a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM)	242:400	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	0	39	theme	okra	149:152	arg1	mucilage					154:161	pectin and okra mucilage	138:161	mucilage	154:161	Quality-by-design approach for development of sustained-release multiple-unit beads of lamotrigine based on ion-cross-linked composite of pectin and okra mucilage: An in vitro appraisal.
32653379	4	40	theme	surface	749:755	arg1	morphology					757:766	The surface morphology	745:766	The surface morphology of the bead before and after dissolution test	745:812	The surface morphology of the bead before and after dissolution test was examined by SEM.
32653379	7	41	theme	excellent	1382:1390	arg1	DEE					1392:1394	excellent DEE	1382:1394	excellent DEE of 89.2 ± 4.4%	1382:1409	The optimized formulation showed excellent DEE of 89.2 ± 4.4% and a sustained release profile with release similarity factor of 94.9.
32653379	8	42	theme	hydration	1566:1574	arg1	mechanism					1544:1552	a release mechanism combined	1534:1561	a release mechanism combined of hydration, diffusion and erosion	1534:1597	Kinetic modeling of drug release data demonstrated a release mechanism combined of hydration, diffusion and erosion.
32653379	2	43	theme	variables	558:566	arg1	levels					530:535	Two different levels	516:535	Two different levels of three independent variables (total polymer concentration, polymer ratio and [CaCl2])	516:623	Two different levels of three independent variables (total polymer concentration, polymer ratio and [CaCl2]) were considered for the experimental design.
32653379	7	44	theme	%	1409:1409	arg1	profile					1435:1441	a sustained release profile	1415:1441	a sustained release profile with release similarity factor of 94.9	1415:1480	The optimized formulation showed excellent DEE of 89.2 ± 4.4% and a sustained release profile with release similarity factor of 94.9.
32653379	7	44	theme	%	1409:1409	arg1	DEE					1392:1394	excellent DEE	1382:1394	excellent DEE of 89.2 ± 4.4%	1382:1409	The optimized formulation showed excellent DEE of 89.2 ± 4.4% and a sustained release profile with release similarity factor of 94.9.
32653379	6	45	theme	Design-Expert	1068:1080	arg1	Version					1092:1098	Version 12	1092:1101	Version 12	1092:1101	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	6	45	theme	Design-Expert	1068:1080	arg1	software					1082:1089	Design-Expert software	1068:1089	Design-Expert software (Version 12) with the targets	1068:1119	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	0	46	theme	sustained-release	46:62	arg1	beads					78:82	sustained-release multiple-unit beads	46:82	sustained-release multiple-unit beads of lamotrigine	46:97	Quality-by-design approach for development of sustained-release multiple-unit beads of lamotrigine based on ion-cross-linked composite of pectin and okra mucilage: An in vitro appraisal.
32653379	2	47	theme	independent	546:556	arg1	variables					558:566	three independent variables	540:566	three independent variables (total polymer concentration, polymer ratio and [CaCl2])	540:623	Two different levels of three independent variables (total polymer concentration, polymer ratio and [CaCl2]) were considered for the experimental design.
32653379	2	47	theme	independent	546:556	arg1	[CaCl2					616:621	[CaCl2	616:621	[CaCl2	616:621	Two different levels of three independent variables (total polymer concentration, polymer ratio and [CaCl2]) were considered for the experimental design.
32653379	2	47	theme	independent	546:556	arg1	concentration					583:595	total polymer concentration	569:595	total polymer concentration	569:595	Two different levels of three independent variables (total polymer concentration, polymer ratio and [CaCl2]) were considered for the experimental design.
32653379	2	47	theme	independent	546:556	arg1	ratio					606:610	polymer ratio	598:610	polymer ratio	598:610	Two different levels of three independent variables (total polymer concentration, polymer ratio and [CaCl2]) were considered for the experimental design.
32653379	6	48	theme	drug	1242:1245	arg1	release					1247:1253	percent drug release	1234:1253	percent drug release at 2 h	1234:1260	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	1	49	theme	present	213:219	arg1	study					221:225	the present study	209:225	the present study	209:225	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	8	50	theme	diffusion	1577:1585	arg1	mechanism					1544:1552	a release mechanism combined	1534:1561	a release mechanism combined of hydration, diffusion and erosion	1534:1597	Kinetic modeling of drug release data demonstrated a release mechanism combined of hydration, diffusion and erosion.
32653379	0	51	theme	mucilage	154:161	arg1	mucilage					154:161	pectin and okra mucilage	138:161	mucilage	154:161	Quality-by-design approach for development of sustained-release multiple-unit beads of lamotrigine based on ion-cross-linked composite of pectin and okra mucilage: An in vitro appraisal.
32653379	0	51	theme	mucilage	154:161	arg1	composite					125:133	ion-cross-linked composite	108:133	ion-cross-linked composite of pectin and okra mucilage	108:161	Quality-by-design approach for development of sustained-release multiple-unit beads of lamotrigine based on ion-cross-linked composite of pectin and okra mucilage: An in vitro appraisal.
32653379	6	52	theme	percent	1234:1240	arg1	release					1247:1253	percent drug release	1234:1253	percent drug release at 2 h	1234:1260	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	1	53	theme	full	493:496	arg1	design					508:513	23 full factorial design	490:513	23 full factorial design	490:513	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	1	54	theme	study	221:225	arg1	objective					196:204	The main objective	187:204	The main objective of the present study	187:225	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	0	55	theme	beads	78:82	arg1	development					31:41	development	31:41	development of sustained-release multiple-unit beads of lamotrigine	31:97	Quality-by-design approach for development of sustained-release multiple-unit beads of lamotrigine based on ion-cross-linked composite of pectin and okra mucilage: An in vitro appraisal.
32653379	1	56	theme	cross-linked	321:332	arg1	pectin					366:371	pectin	366:371	pectin (PTN)	366:377	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	1	56	theme	cross-linked	321:332	arg1	mucilage					388:395	okra mucilage	383:395	okra mucilage (OM)	383:400	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	1	56	theme	cross-linked	321:332	arg1	polysaccharides					342:356	ionotropically cross-linked natural polysaccharides	306:356	ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM)	306:400	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	0	57	theme	in	167:168	arg1	appraisal					176:184	An in vitro appraisal	164:184	An in vitro appraisal	164:184	Quality-by-design approach for development of sustained-release multiple-unit beads of lamotrigine based on ion-cross-linked composite of pectin and okra mucilage: An in vitro appraisal.
32653379	1	58	theme	factorial	498:506	arg1	design					508:513	23 full factorial design	490:513	23 full factorial design	490:513	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	0	59	dep	appraisal	176:184	arg1	approach					18:25	Quality-by-design approach	0:25	Quality-by-design approach for development of sustained-release multiple-unit beads of lamotrigine based on ion-cross-linked composite of pectin and okra mucilage	0:161	Quality-by-design approach for development of sustained-release multiple-unit beads of lamotrigine based on ion-cross-linked composite of pectin and okra mucilage: An in vitro appraisal.
32653379	0	60	theme	multiple-unit	64:76	arg1	beads					78:82	sustained-release multiple-unit beads	46:82	sustained-release multiple-unit beads of lamotrigine	46:97	Quality-by-design approach for development of sustained-release multiple-unit beads of lamotrigine based on ion-cross-linked composite of pectin and okra mucilage: An in vitro appraisal.
32653379	1	61	theme	natural	334:340	arg1	pectin					366:371	pectin	366:371	pectin (PTN)	366:377	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	1	61	theme	natural	334:340	arg1	mucilage					388:395	okra mucilage	383:395	okra mucilage (OM)	383:400	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	1	61	theme	natural	334:340	arg1	polysaccharides					342:356	ionotropically cross-linked natural polysaccharides	306:356	ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM)	306:400	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	6	62	theme	difference	1329:1338	arg1	factors					1340:1346	difference factors	1329:1346	difference factors	1329:1346	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	6	63	theme	release	1143:1149	arg1	profile					1151:1157	USP-reference release profile	1129:1157	USP-reference release profile	1129:1157	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	2	64	theme	polymer	575:581	arg1	variables					558:566	three independent variables	540:566	three independent variables (total polymer concentration, polymer ratio and [CaCl2])	540:623	Two different levels of three independent variables (total polymer concentration, polymer ratio and [CaCl2]) were considered for the experimental design.
32653379	2	64	theme	polymer	575:581	arg1	concentration					583:595	total polymer concentration	569:595	total polymer concentration	569:595	Two different levels of three independent variables (total polymer concentration, polymer ratio and [CaCl2]) were considered for the experimental design.
32653379	1	65	link	cross-linked	321:332	arg1	pectin					366:371	pectin	366:371	pectin (PTN)	366:377	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	1	65	link	cross-linked	321:332	arg1	mucilage					388:395	okra mucilage	383:395	okra mucilage (OM)	383:400	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	1	65	link	cross-linked	321:332	arg1	polysaccharides					342:356	ionotropically cross-linked natural polysaccharides	306:356	ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM)	306:400	The main objective of the present study was to develop a sustained release multiple-unit beads of lamotrigine based on ionotropically cross-linked natural polysaccharides such as pectin (PTN) and okra mucilage (OM) and optimize the polymer-concentration, polymer ratio and cross-linker concentration by 23 full factorial design.
32653379	0	66	dep	in	167:168	arg1	vitro					170:174	vitro	170:174	vitro	170:174	Quality-by-design approach for development of sustained-release multiple-unit beads of lamotrigine based on ion-cross-linked composite of pectin and okra mucilage: An in vitro appraisal.
32653379	5	67	theme	release	947:953	arg1	similarity					955:964	release similarity	947:964	release similarity	947:964	Effects of the independent variables on bead-size, drug-encapsulation-efficiency (DEE), drug-release along with release similarity and difference factors were examined.
32653379	6	68	theme	release	1306:1312	arg1	similarity					1314:1323	release similarity	1306:1323	release similarity	1306:1323	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	2	69	theme	total	569:573	arg1	variables					558:566	three independent variables	540:566	three independent variables (total polymer concentration, polymer ratio and [CaCl2])	540:623	Two different levels of three independent variables (total polymer concentration, polymer ratio and [CaCl2]) were considered for the experimental design.
32653379	2	69	theme	total	569:573	arg1	concentration					583:595	total polymer concentration	569:595	total polymer concentration	569:595	Two different levels of three independent variables (total polymer concentration, polymer ratio and [CaCl2]) were considered for the experimental design.
32653379	6	70	with	software	1082:1089	arg1	targets					1113:1119	the targets	1109:1119	the targets	1109:1119	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	5	71	from	Effects	835:841	arg1	drug-encapsulation-efficiency					886:914	drug-encapsulation-efficiency	886:914	drug-encapsulation-efficiency (DEE)	886:920	Effects of the independent variables on bead-size, drug-encapsulation-efficiency (DEE), drug-release along with release similarity and difference factors were examined.
32653379	5	71	from	Effects	835:841	arg1	bead-size					875:883	bead-size	875:883	bead-size	875:883	Effects of the independent variables on bead-size, drug-encapsulation-efficiency (DEE), drug-release along with release similarity and difference factors were examined.
32653379	5	71	from	Effects	835:841	arg1	factors					981:987	difference factors	970:987	difference factors	970:987	Effects of the independent variables on bead-size, drug-encapsulation-efficiency (DEE), drug-release along with release similarity and difference factors were examined.
32653379	5	71	from	Effects	835:841	arg1	DEE					917:919	DEE	917:919	DEE	917:919	Effects of the independent variables on bead-size, drug-encapsulation-efficiency (DEE), drug-release along with release similarity and difference factors were examined.
32653379	5	71	from	Effects	835:841	arg1	drug-release					923:934	drug-release	923:934	drug-release along with release similarity	923:964	Effects of the independent variables on bead-size, drug-encapsulation-efficiency (DEE), drug-release along with release similarity and difference factors were examined.
32653379	6	72	theme	USP-reference	1129:1141	arg1	profile					1151:1157	USP-reference release profile	1129:1157	USP-reference release profile	1129:1157	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	0	73	theme	lamotrigine	87:97	arg1	beads					78:82	sustained-release multiple-unit beads	46:82	sustained-release multiple-unit beads of lamotrigine	46:97	Quality-by-design approach for development of sustained-release multiple-unit beads of lamotrigine based on ion-cross-linked composite of pectin and okra mucilage: An in vitro appraisal.
32653379	3	74	theme	Drug-polymers	670:682	arg1	compatibility					684:696	Drug-polymers compatibility	670:696	Drug-polymers compatibility	670:696	Drug-polymers compatibility was examined by FTIR, DSC, TGA and powder-XRD.
32653379	4	75	theme	dissolution	797:807	arg1	test					809:812	dissolution test	797:812	dissolution test	797:812	The surface morphology of the bead before and after dissolution test was examined by SEM.
32653379	7	76	with	profile	1435:1441	arg1	factor					1467:1472	release similarity factor	1448:1472	release similarity factor of 94.9	1448:1480	The optimized formulation showed excellent DEE of 89.2 ± 4.4% and a sustained release profile with release similarity factor of 94.9.
32653379	5	77	theme	independent	850:860	arg1	variables					862:870	the independent variables	846:870	the independent variables	846:870	Effects of the independent variables on bead-size, drug-encapsulation-efficiency (DEE), drug-release along with release similarity and difference factors were examined.
32653379	6	78	theme	response	1201:1208	arg1	similarity					1314:1323	release similarity	1306:1323	release similarity	1306:1323	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	6	78	theme	response	1201:1208	arg1	release					1247:1253	percent drug release	1234:1253	percent drug release at 2 h	1234:1260	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	6	78	theme	response	1201:1208	arg1	DEE					1229:1231	DEE	1229:1231	DEE	1229:1231	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	6	78	theme	response	1201:1208	arg1	constant					1296:1303	Korsmeyer-Peppas rate constant	1274:1303	Korsmeyer-Peppas rate constant	1274:1303	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	6	78	theme	response	1201:1208	arg1	factors					1340:1346	difference factors	1329:1346	difference factors	1329:1346	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	6	78	theme	response	1201:1208	arg1	5 h					1263:1265	5 h	1263:1265	5 h	1263:1265	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	6	78	theme	response	1201:1208	arg1	12 h					1268:1271	12 h	1268:1271	12 h	1268:1271	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	6	78	theme	response	1201:1208	arg1	parameters					1210:1219	all the response parameters	1193:1219	all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors	1193:1346	The independent variables were then numerically optimized using Design-Expert software (Version 12) with the targets to meet USP-reference release profile after the analysis of variance of all the response parameters such as DEE, percent drug release at 2 h, 5 h, 12 h, Korsmeyer-Peppas rate constant, release similarity and difference factors.
32653379	8	79	theme	release	1508:1514	arg1	data					1516:1519	drug release data	1503:1519	drug release data	1503:1519	Kinetic modeling of drug release data demonstrated a release mechanism combined of hydration, diffusion and erosion.
32511307	0	0	theme	immune	73:78	arg1	recognition					80:90	immune recognition	73:90	immune recognition	73:90	Analysis of the SARS-CoV-2 spike protein glycan shield: implications for immune recognition.
32511307	4	1	theme	immune	907:912	arg1	response					914:921	the adaptive immune response	894:921	the adaptive immune response to the SARS-CoV-2 virus or to a related vaccine	894:969	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.
32511307	4	2	theme	related	955:961	arg1	vaccine					963:969	a related vaccine	953:969	a related vaccine	953:969	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.
32511307	2	3	theme	nascent	360:366	arg1	glycoproteins					368:380	the nascent glycoproteins	356:380	the nascent glycoproteins (prior to enzymatic modifications in the Golgi)	356:428	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.
32511307	6	4	gly	glycoform	1334:1342	arg1	HEK293					1327:1332	the HEK293 glycoform	1323:1342	the HEK293 glycoform	1323:1342	Despite the relatively modest contribution of the glycans to the total molecular weight (17% for the HEK293 glycoform) the level of surface shielding is disproportionately high at 42%.
32511307	4	5	theme	antigen	795:801	arg1	complexes					809:817	human leukocyte antigen (HLA) complexes	779:817	human leukocyte antigen (HLA) complexes	779:817	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.
32511307	2	6	from	viruses	504:510	arg1	present					487:493	present	487:493	present	487:493	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.
32511307	4	7	theme	glycosylation	854:866	arg1	role					836:839	the role	832:839	the role of S protein glycosylation in potentially modulating the adaptive immune response to the SARS-CoV-2 virus or to a related vaccine	832:969	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.
32511307	5	8	theme	antibody	1049:1056	arg1	recognition					1058:1068	antibody recognition	1049:1068	antibody recognition by glycans	1049:1079	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.
32511307	2	9	gly	glycoproteins	368:380	arg1	glycoproteins					368:380	the nascent glycoproteins	356:380	the nascent glycoproteins (prior to enzymatic modifications in the Golgi)	356:428	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.
32511307	4	10	from	peptides	713:720	arg1	glycoprotein					731:742	the S glycoprotein	725:742	the S glycoprotein	725:742	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.
32511307	1	11	theme	glycomics	227:235	arg1	data					237:240	glycomics data	227:240	glycomics data	227:240	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.
32511307	4	12	theme	protein	846:852	arg1	glycosylation					854:866	S protein glycosylation	844:866	S protein glycosylation	844:866	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.
32511307	3	13	theme	S	670:670	arg1	glycoprotein					672:683	the S glycoprotein	666:683	the S glycoprotein	666:683	These models were subjected to molecular dynamics (MD) simulation to determine the extent to which glycan microheterogeneity impacts the antigenicity of the S glycoprotein.
32511307	6	14	from	%	1408:1408	arg1	level					1349:1353	the level	1345:1353	the level of surface shielding	1345:1374	Despite the relatively modest contribution of the glycans to the total molecular weight (17% for the HEK293 glycoform) the level of surface shielding is disproportionately high at 42%.
32511307	6	14	from	%	1408:1408	arg1	high					1398:1401	high	1398:1401	high	1398:1401	Despite the relatively modest contribution of the glycans to the total molecular weight (17% for the HEK293 glycoform) the level of surface shielding is disproportionately high at 42%.
32511307	5	15	theme	binding	1122:1128	arg1	domain					1130:1135	the ACE2 receptor binding domain	1104:1135	the ACE2 receptor binding domain	1104:1135	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.
32511307	3	16	theme	glycoprotein	672:683	arg1	antigenicity					650:661	the antigenicity	646:661	the antigenicity of the S glycoprotein	646:683	These models were subjected to molecular dynamics (MD) simulation to determine the extent to which glycan microheterogeneity impacts the antigenicity of the S glycoprotein.
32511307	1	17	from	glycoforms	133:142	arg1	SARS-CoV-2					179:188	SARS-CoV-2	179:188	SARS-CoV-2	179:188	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.
32511307	1	18	theme	spike	151:155	arg1	glycoprotein					161:172	the spike (S) glycoprotein	147:172	the spike (S) glycoprotein from SARS-CoV-2	147:188	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.
32511307	6	19	theme	modest	1249:1254	arg1	contribution					1256:1267	the relatively modest contribution	1234:1267	the relatively modest contribution of the glycans to the total molecular weight (17% for the HEK293 glycoform)	1234:1343	Despite the relatively modest contribution of the glycans to the total molecular weight (17% for the HEK293 glycoform) the level of surface shielding is disproportionately high at 42%.
32511307	1	20	gly	glycoprotein	161:172	arg1	glycoprotein					161:172	the spike (S) glycoprotein	147:172	the spike (S) glycoprotein from SARS-CoV-2	147:188	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.
32511307	6	21	theme	HEK293	1327:1332	arg1	glycoform					1334:1342	the HEK293 glycoform	1323:1342	the HEK293 glycoform	1323:1342	Despite the relatively modest contribution of the glycans to the total molecular weight (17% for the HEK293 glycoform) the level of surface shielding is disproportionately high at 42%.
32511307	4	22	theme	adaptive	898:905	arg1	response					914:921	the adaptive immune response	894:921	the adaptive immune response to the SARS-CoV-2 virus or to a related vaccine	894:969	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.
32511307	4	23	theme	leukocyte	785:793	arg1	HLA					804:806	HLA	804:806	HLA	804:806	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.
32511307	4	23	theme	leukocyte	785:793	arg1	antigen					795:801	human leukocyte antigen	779:801	human leukocyte antigen (HLA) complexes	779:817	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.
32511307	5	24	theme	protein	1004:1010	arg1	surface					1012:1018	the protein surface	1000:1018	the protein surface	1000:1018	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.
32511307	1	25	theme	S	158:158	arg1	glycoprotein					161:172	the spike (S) glycoprotein	147:172	the spike (S) glycoprotein from SARS-CoV-2	147:188	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.
32511307	4	26	theme	S	844:844	arg1	glycosylation					854:866	S protein glycosylation	844:866	S protein glycosylation	844:866	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.
32511307	3	27	gly	glycoprotein	672:683	arg1	glycoprotein					672:683	the S glycoprotein	666:683	the S glycoprotein	666:683	These models were subjected to molecular dynamics (MD) simulation to determine the extent to which glycan microheterogeneity impacts the antigenicity of the S glycoprotein.
32511307	4	28	theme	human	779:783	arg1	HLA					804:806	HLA	804:806	HLA	804:806	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.
32511307	4	28	theme	human	779:783	arg1	antigen					795:801	human leukocyte antigen	779:801	human leukocyte antigen (HLA) complexes	779:817	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.
32511307	0	29	theme	SARS-CoV-2	16:25	arg1	glycan					41:46	the SARS-CoV-2 spike protein glycan shield	12:53	the SARS-CoV-2 spike protein glycan shield	12:53	Analysis of the SARS-CoV-2 spike protein glycan shield: implications for immune recognition.
32511307	2	30	theme	other	498:502	arg1	viruses					504:510	other viruses	498:510	other viruses	498:510	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.
32511307	6	31	theme	glycans	1276:1282	arg1	contribution					1256:1267	the relatively modest contribution	1234:1267	the relatively modest contribution of the glycans to the total molecular weight (17% for the HEK293 glycoform)	1234:1343	Despite the relatively modest contribution of the glycans to the total molecular weight (17% for the HEK293 glycoform) the level of surface shielding is disproportionately high at 42%.
32511307	1	32	theme	glycoprotein	161:172	arg1	glycoforms					133:142	glycoforms	133:142	glycoforms of the spike (S) glycoprotein from SARS-CoV-2	133:188	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.
32511307	2	33	theme	enzymatic	392:400	arg1	modifications					402:414	enzymatic modifications	392:414	enzymatic modifications in the Golgi	392:427	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.
32511307	5	34	theme	3D	976:977	arg1	structures					979:988	The 3D structures	972:988	The 3D structures	972:988	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.
32511307	5	35	theme	specific	1206:1213	arg1	glycoform					1215:1223	the specific glycoform	1202:1223	the specific glycoform	1202:1223	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.
32511307	2	36	theme	prior	383:387	arg1	glycoproteins					368:380	the nascent glycoproteins	356:380	the nascent glycoproteins (prior to enzymatic modifications in the Golgi)	356:428	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.
32511307	0	37	theme	protein	33:39	arg1	glycan					41:46	the SARS-CoV-2 spike protein glycan shield	12:53	the SARS-CoV-2 spike protein glycan shield	12:53	Analysis of the SARS-CoV-2 spike protein glycan shield: implications for immune recognition.
32511307	2	38	theme	present	487:493	arg1	antigens					478:485	antigens	478:485	antigens present in other viruses	478:510	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.
32511307	5	39	theme	domain	1130:1135	arg1	exception					1091:1099	the exception	1087:1099	the exception of the ACE2 receptor binding domain	1087:1135	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.
32511307	2	40	attach	present	487:493	arg2	antigens					478:485	antigens	478:485	antigens present in other viruses	478:510	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.
32511307	2	40	attach	present	487:493	arg1	viruses					504:510	other viruses	498:510	other viruses	498:510	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.
32511307	0	41	theme	spike	27:31	arg1	glycan					41:46	the SARS-CoV-2 spike protein glycan shield	12:53	the SARS-CoV-2 spike protein glycan shield	12:53	Analysis of the SARS-CoV-2 spike protein glycan shield: implications for immune recognition.
32511307	5	42	theme	ACE2	1108:1111	arg1	domain					1130:1135	the ACE2 receptor binding domain	1104:1135	the ACE2 receptor binding domain	1104:1135	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.
32511307	0	43	dep	glycan	41:46	arg1	shield					48:53	shield	48:53	shield	48:53	Analysis of the SARS-CoV-2 spike protein glycan shield: implications for immune recognition.
32511307	1	44	from	SARS-CoV-2	179:188	arg1	glycoforms					133:142	glycoforms	133:142	glycoforms of the spike (S) glycoprotein from SARS-CoV-2	133:188	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.
32511307	1	44	from	SARS-CoV-2	179:188	arg1	glycoprotein					161:172	the spike (S) glycoprotein	147:172	the spike (S) glycoprotein from SARS-CoV-2	147:188	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.
32511307	4	45	gly	glycoprotein	731:742	arg1	glycoprotein					731:742	the S glycoprotein	725:742	the S glycoprotein	725:742	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.
32511307	1	46	theme	HEK293	270:275	arg1	cells					277:281	HEK293 cells	270:281	HEK293 cells	270:281	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.
32511307	1	47	gly	glycoforms	133:142	arg1	glycoprotein					161:172	the spike (S) glycoprotein	147:172	the spike (S) glycoprotein from SARS-CoV-2	147:188	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.
32511307	2	48	from	present	345:351	arg1	glycoproteins					368:380	the nascent glycoproteins	356:380	the nascent glycoproteins (prior to enzymatic modifications in the Golgi)	356:428	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.
32511307	0	49	theme	glycan	41:46	arg1	Analysis					0:7	Analysis	0:7	Analysis of the SARS-CoV-2 spike protein glycan shield: implications for immune recognition.	0:91	Analysis of the SARS-CoV-2 spike protein glycan shield: implications for immune recognition.
32511307	6	50	dep	weight	1307:1312	arg1	%					1317:1317	17%	1315:1317	17% for the HEK293 glycoform	1315:1342	Despite the relatively modest contribution of the glycans to the total molecular weight (17% for the HEK293 glycoform) the level of surface shielding is disproportionately high at 42%.
32511307	5	51	theme	shielding	1166:1174	arg1	degree					1156:1161	the degree	1152:1161	the degree of shielding	1152:1174	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.
32511307	5	51	theme	shielding	1166:1174	arg1	insensitive					1187:1197	insensitive	1187:1197	insensitive	1187:1197	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.
32511307	3	52	theme	glycan	612:617	arg1	microheterogeneity					619:636	glycan microheterogeneity	612:636	glycan microheterogeneity	612:636	These models were subjected to molecular dynamics (MD) simulation to determine the extent to which glycan microheterogeneity impacts the antigenicity of the S glycoprotein.
32511307	4	53	theme	S	729:729	arg1	glycoprotein					731:742	the S glycoprotein	725:742	the S glycoprotein	725:742	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.
32511307	3	54	theme	molecular	544:552	arg1	MD					564:565	MD	564:565	MD	564:565	These models were subjected to molecular dynamics (MD) simulation to determine the extent to which glycan microheterogeneity impacts the antigenicity of the S glycoprotein.
32511307	3	54	theme	molecular	544:552	arg1	dynamics					554:561	molecular dynamics	544:561	molecular dynamics (MD) simulation	544:577	These models were subjected to molecular dynamics (MD) simulation to determine the extent to which glycan microheterogeneity impacts the antigenicity of the S glycoprotein.
32511307	1	55	theme	3D	116:117	arg1	structures					119:128	3D structures	116:128	3D structures	116:128	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.
32511307	6	56	theme	shielding	1366:1374	arg1	level					1349:1353	the level	1345:1353	the level of surface shielding	1345:1374	Despite the relatively modest contribution of the glycans to the total molecular weight (17% for the HEK293 glycoform) the level of surface shielding is disproportionately high at 42%.
32511307	6	56	theme	shielding	1366:1374	arg1	high					1398:1401	high	1398:1401	high	1398:1401	Despite the relatively modest contribution of the glycans to the total molecular weight (17% for the HEK293 glycoform) the level of surface shielding is disproportionately high at 42%.
32511307	2	57	from	present	487:493	arg1	viruses					504:510	other viruses	498:510	other viruses	498:510	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.
32511307	2	58	attach	present	345:351	arg2	those					339:343	those	339:343	those	339:343	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.
32511307	2	58	attach	present	345:351	arg1	glycoproteins					368:380	the nascent glycoproteins	356:380	the nascent glycoproteins (prior to enzymatic modifications in the Golgi)	356:428	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.
32511307	2	59	located	observed	466:473	arg1	antigens					478:485	antigens	478:485	antigens present in other viruses	478:510	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.
32511307	2	59	located	observed	466:473	arg2	those					442:446	those	442:446	those	442:446	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.
32511307	6	60	theme	surface	1358:1364	arg1	shielding					1366:1374	surface shielding	1358:1374	surface shielding	1358:1374	Despite the relatively modest contribution of the glycans to the total molecular weight (17% for the HEK293 glycoform) the level of surface shielding is disproportionately high at 42%.
32511307	6	61	theme	total	1291:1295	arg1	weight					1307:1312	the total molecular weight	1287:1312	the total molecular weight (17% for the HEK293 glycoform)	1287:1343	Despite the relatively modest contribution of the glycans to the total molecular weight (17% for the HEK293 glycoform) the level of surface shielding is disproportionately high at 42%.
32511307	3	62	theme	dynamics	554:561	arg1	simulation					568:577	molecular dynamics (MD) simulation	544:577	molecular dynamics (MD) simulation	544:577	These models were subjected to molecular dynamics (MD) simulation to determine the extent to which glycan microheterogeneity impacts the antigenicity of the S glycoprotein.
32511307	2	63	from	glycoproteins	368:380	arg1	present					345:351	present	345:351	present	345:351	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.
32511307	6	64	theme	molecular	1297:1305	arg1	weight					1307:1312	the total molecular weight	1287:1312	the total molecular weight (17% for the HEK293 glycoform)	1287:1343	Despite the relatively modest contribution of the glycans to the total molecular weight (17% for the HEK293 glycoform) the level of surface shielding is disproportionately high at 42%.
32511307	1	65	theme	reported	200:207	arg1	structures					212:221	reported 3D structures	200:221	reported 3D structures	200:221	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.
32511307	4	66	theme	SARS-CoV-2	930:939	arg1	virus					941:945	the SARS-CoV-2 virus	926:945	the SARS-CoV-2 virus	926:945	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.
32511307	2	67	from	modifications	402:414	arg1	Golgi					423:427	the Golgi	419:427	the Golgi	419:427	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.
32511307	0	68	dep	Analysis	0:7	arg1	implications					56:67	implications	56:67	Analysis of the SARS-CoV-2 spike protein glycan shield: implications for immune recognition.	0:91	Analysis of the SARS-CoV-2 spike protein glycan shield: implications for immune recognition.
32511307	5	69	theme	receptor	1113:1120	arg1	domain					1130:1135	the ACE2 receptor binding domain	1104:1135	the ACE2 receptor binding domain	1104:1135	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.
32511307	1	70	theme	glycoforms	133:142	arg1	structures					119:128	3D structures	116:128	3D structures	116:128	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.
32511307	1	71	theme	3D	209:210	arg1	structures					212:221	reported 3D structures	200:221	reported 3D structures	200:221	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.
34740855	8	0	theme	mineral	1097:1103	arg1	matrix					1117:1122	mineral and organic matrix	1097:1122	mineral and organic matrix	1097:1122	Using a deconstruction method to dissect the contribution of mineral and organic matrix, we find that the damping factor of the organic matrix does deteriorate.
34740855	14	1	theme	teeth	2210:2214	arg1	features					2198:2205	the protective, biomechanical, and regenerative features	2150:2205	the protective, biomechanical, and regenerative features of teeth	2150:2214	Dentin is integral to the protective, biomechanical, and regenerative features of teeth.
34740855	8	2	theme	organic	1109:1115	arg1	matrix					1117:1122	mineral and organic matrix	1097:1122	mineral and organic matrix	1097:1122	Using a deconstruction method to dissect the contribution of mineral and organic matrix, we find that the damping factor of the organic matrix does deteriorate.
34740855	4	3	theme	system	550:555	arg1	stresses					523:530	mechanical load stresses	507:530	mechanical load stresses of the tooth-bone system	507:555	Root dentin is integral to tooth function in that it anchors and dissipates mechanical load stresses of the tooth-bone system.
34740855	12	4	dep	STATEMENT	1898:1906	arg1	years					1979:1983	65 years old	1976:1987	65 years old	1976:1987	STATEMENT OF SIGNIFICANCE: Globally, a quarter of the population will be over 65 years old by 2050.
34740855	11	5	theme	altered	1652:1658	arg1	interactions					1675:1686	altered matrix-mineral interactions	1652:1686	altered matrix-mineral interactions	1652:1686	To explain this discrepancy, we propose that altered matrix-mineral interactions, possibly mediated by carbonate ions sequestered at the mineral interface and/or altered glycosaminoglycans counteract the deleterious effects of aging on the structural components of the extracellular matrix.
34740855	2	6	theme	human	251:255	arg1	teeth					257:261	human teeth	251:261	human teeth	251:261	While resilient, human teeth undergo wear and disease, affecting overall physical, psychological, and social human health.
34740855	15	7	theme	altered	2274:2280	arg1	properties					2293:2302	altered mechanical properties	2274:2302	altered mechanical properties	2274:2302	Here, we demonstrate that older root dentin not only has altered mechanical properties, but shows characteristic shifts in mineralization, composition, and post-translational modifications of the matrix.
34740855	5	8	theme	small	728:732	arg1	scattering					746:755	small angle X-ray scattering	728:755	small angle X-ray scattering	728:755	Here, we assess the viscoelastic behavior, composition, and ultrastructure of young and old root dentin using nano-dynamic mechanical analysis, micro-Raman spectroscopy, small angle X-ray scattering, atomic force and transmission electron microscopies.
34740855	16	9	theme	mechanistic	2460:2470	arg1	link					2472:2475	a mechanistic link	2458:2475	a mechanistic link between mineral and matrix components to the biomechanical performance of aging dentin with implications for efforts to slow or even reverse the aging process	2458:2634	This strongly suggests that there is a mechanistic link between mineral and matrix components to the biomechanical performance of aging dentin with implications for efforts to slow or even reverse the aging process.
34740855	8	10	theme	matrix	1172:1177	arg1	factor					1150:1155	the damping factor	1138:1155	the damping factor of the organic matrix	1138:1177	Using a deconstruction method to dissect the contribution of mineral and organic matrix, we find that the damping factor of the organic matrix does deteriorate.
34740855	10	11	theme	root	1552:1555	arg1	dentin					1557:1562	root dentin	1552:1562	root dentin	1552:1562	Therefore, even in the absence of remodeling, the extracellular matrix of root dentin shares traits of aging with other tissues.
34740855	15	12	theme	mechanical	2282:2291	arg1	properties					2293:2302	altered mechanical properties	2274:2302	altered mechanical properties	2274:2302	Here, we demonstrate that older root dentin not only has altered mechanical properties, but shows characteristic shifts in mineralization, composition, and post-translational modifications of the matrix.
34740855	6	13	theme	dentin	833:838	arg1	stiffness					848:856	the root dentin overall stiffness	824:856	the root dentin overall stiffness	824:856	We find that the root dentin overall stiffness increases with age.
34740855	11	14	from	effects	1823:1829	arg1	components					1858:1867	the structural components	1843:1867	the structural components of the extracellular matrix	1843:1895	To explain this discrepancy, we propose that altered matrix-mineral interactions, possibly mediated by carbonate ions sequestered at the mineral interface and/or altered glycosaminoglycans counteract the deleterious effects of aging on the structural components of the extracellular matrix.
34740855	14	15	theme	biomechanical	2166:2178	arg1	features					2198:2205	the protective, biomechanical, and regenerative features	2150:2205	the protective, biomechanical, and regenerative features of teeth	2150:2214	Dentin is integral to the protective, biomechanical, and regenerative features of teeth.
34740855	14	16	theme	protective	2154:2163	arg1	features					2198:2205	the protective, biomechanical, and regenerative features	2150:2205	the protective, biomechanical, and regenerative features of teeth	2150:2214	Dentin is integral to the protective, biomechanical, and regenerative features of teeth.
34740855	5	17	theme	root	650:653	arg1	dentin					655:660	young and old root dentin	636:660	young and old root dentin	636:660	Here, we assess the viscoelastic behavior, composition, and ultrastructure of young and old root dentin using nano-dynamic mechanical analysis, micro-Raman spectroscopy, small angle X-ray scattering, atomic force and transmission electron microscopies.
34740855	9	18	theme	glycosaminoglycans	1457:1474	arg1	pattern					1446:1452	sulfation pattern	1436:1452	sulfation pattern of glycosaminoglycans	1436:1474	Compositional and ultrastructural analyses revealed higher mineral-to-matrix ratio, altered enzymatic and non-enzymatic collagen cross-linking, increased collagen d-spacing and fibril diameter, and decreased abundance of proteoglycans and sulfation pattern of glycosaminoglycans .
34740855	9	18	theme	glycosaminoglycans	1457:1474	arg1	abundance					1405:1413	abundance	1405:1413	abundance of proteoglycans	1405:1430	Compositional and ultrastructural analyses revealed higher mineral-to-matrix ratio, altered enzymatic and non-enzymatic collagen cross-linking, increased collagen d-spacing and fibril diameter, and decreased abundance of proteoglycans and sulfation pattern of glycosaminoglycans .
34740855	7	19	theme	dentin	965:970	arg1	ability					949:955	the ability	945:955	the ability of root dentin to dissipate energy during deformation	945:1009	Unlike other mineralized tissues and even coronal dentin, however, the ability of root dentin to dissipate energy during deformation does not decay with age.
34740855	16	20	theme	aging	2622:2626	arg1	process					2628:2634	the aging process	2618:2634	the aging process	2618:2634	This strongly suggests that there is a mechanistic link between mineral and matrix components to the biomechanical performance of aging dentin with implications for efforts to slow or even reverse the aging process.
34740855	11	21	theme	aging	1834:1838	arg1	effects					1823:1829	the deleterious effects	1807:1829	the deleterious effects of aging on the structural components of the extracellular matrix	1807:1895	To explain this discrepancy, we propose that altered matrix-mineral interactions, possibly mediated by carbonate ions sequestered at the mineral interface and/or altered glycosaminoglycans counteract the deleterious effects of aging on the structural components of the extracellular matrix.
34740855	11	22	theme	mineral	1744:1750	arg1	interface					1752:1760	the mineral interface	1740:1760	the mineral interface	1740:1760	To explain this discrepancy, we propose that altered matrix-mineral interactions, possibly mediated by carbonate ions sequestered at the mineral interface and/or altered glycosaminoglycans counteract the deleterious effects of aging on the structural components of the extracellular matrix.
34740855	16	23	theme	aging	2551:2555	arg1	dentin					2557:2562	aging dentin	2551:2562	aging dentin	2551:2562	This strongly suggests that there is a mechanistic link between mineral and matrix components to the biomechanical performance of aging dentin with implications for efforts to slow or even reverse the aging process.
34740855	15	24	from	shifts	2330:2335	arg1	mineralization					2340:2353	mineralization	2340:2353	mineralization	2340:2353	Here, we demonstrate that older root dentin not only has altered mechanical properties, but shows characteristic shifts in mineralization, composition, and post-translational modifications of the matrix.
34740855	15	24	from	shifts	2330:2335	arg1	modifications					2392:2404	post-translational modifications	2373:2404	post-translational modifications of the matrix	2373:2418	Here, we demonstrate that older root dentin not only has altered mechanical properties, but shows characteristic shifts in mineralization, composition, and post-translational modifications of the matrix.
34740855	15	24	from	shifts	2330:2335	arg1	composition					2356:2366	composition	2356:2366	composition	2356:2366	Here, we demonstrate that older root dentin not only has altered mechanical properties, but shows characteristic shifts in mineralization, composition, and post-translational modifications of the matrix.
34740855	5	25	theme	transmission	775:786	arg1	microscopies					797:808	transmission electron microscopies	775:808	transmission electron microscopies	775:808	Here, we assess the viscoelastic behavior, composition, and ultrastructure of young and old root dentin using nano-dynamic mechanical analysis, micro-Raman spectroscopy, small angle X-ray scattering, atomic force and transmission electron microscopies.
34740855	1	26	theme	physiological	117:129	arg1	Aging					106:110	Aging	106:110	Aging	106:110	Aging is a physiological process with profound impact on the biology and function of biosystems, including the human dentition.
34740855	1	26	theme	physiological	117:129	arg1	process					131:137	a physiological process	115:137	a physiological process with profound impact on the biology and function of biosystems, including the human dentition	115:231	Aging is a physiological process with profound impact on the biology and function of biosystems, including the human dentition.
34740855	15	27	theme	root	2249:2252	arg1	dentin					2254:2259	older root dentin	2243:2259	older root dentin	2243:2259	Here, we demonstrate that older root dentin not only has altered mechanical properties, but shows characteristic shifts in mineralization, composition, and post-translational modifications of the matrix.
34740855	0	28	theme	matrix	56:61	arg1	counterbalance					63:76	the mineral and extracellular matrix counterbalance	26:76	the mineral and extracellular matrix counterbalance	26:76	The stoic tooth root: how the mineral and extracellular matrix counterbalance to keep aged dentin stable.
34740855	9	29	theme	higher	1249:1254	arg1	ratio					1274:1278	higher mineral-to-matrix ratio	1249:1278	higher mineral-to-matrix ratio	1249:1278	Compositional and ultrastructural analyses revealed higher mineral-to-matrix ratio, altered enzymatic and non-enzymatic collagen cross-linking, increased collagen d-spacing and fibril diameter, and decreased abundance of proteoglycans and sulfation pattern of glycosaminoglycans .
34740855	16	30	theme	matrix	2497:2502	arg1	components					2504:2513	matrix components	2497:2513	matrix components	2497:2513	This strongly suggests that there is a mechanistic link between mineral and matrix components to the biomechanical performance of aging dentin with implications for efforts to slow or even reverse the aging process.
34740855	5	31	theme	mechanical	681:690	arg1	analysis					692:699	nano-dynamic mechanical analysis	668:699	nano-dynamic mechanical analysis	668:699	Here, we assess the viscoelastic behavior, composition, and ultrastructure of young and old root dentin using nano-dynamic mechanical analysis, micro-Raman spectroscopy, small angle X-ray scattering, atomic force and transmission electron microscopies.
34740855	7	32	theme	even	915:918	arg1	dentin					928:933	even coronal dentin	915:933	even coronal dentin	915:933	Unlike other mineralized tissues and even coronal dentin, however, the ability of root dentin to dissipate energy during deformation does not decay with age.
34740855	4	33	theme	Root	431:434	arg1	dentin					436:441	Root dentin	431:441	Root dentin	431:441	Root dentin is integral to tooth function in that it anchors and dissipates mechanical load stresses of the tooth-bone system.
34740855	3	34	theme	tooth	395:399	arg1	aging					401:405	tooth aging	395:405	tooth aging	395:405	However, the underlying mechanisms of tooth aging remain largely unknown.
34740855	12	35	theme	SIGNIFICANCE	1911:1922	arg1	STATEMENT					1898:1906	STATEMENT	1898:1906	STATEMENT OF SIGNIFICANCE: Globally, a quarter of the population will be over 65 years old by 2050.	1898:1996	STATEMENT OF SIGNIFICANCE: Globally, a quarter of the population will be over 65 years old by 2050.
34740855	14	36	theme	regenerative	2185:2196	arg1	features					2198:2205	the protective, biomechanical, and regenerative features	2150:2205	the protective, biomechanical, and regenerative features of teeth	2150:2214	Dentin is integral to the protective, biomechanical, and regenerative features of teeth.
34740855	0	37	theme	extracellular	42:54	arg1	counterbalance					63:76	the mineral and extracellular matrix counterbalance	26:76	the mineral and extracellular matrix counterbalance	26:76	The stoic tooth root: how the mineral and extracellular matrix counterbalance to keep aged dentin stable.
34740855	2	38	theme	human	343:347	arg1	health					349:354	overall physical, psychological, and social human health	299:354	overall physical, psychological, and social human health	299:354	While resilient, human teeth undergo wear and disease, affecting overall physical, psychological, and social human health.
34740855	7	39	theme	other	885:889	arg1	tissues					903:909	other mineralized tissues	885:909	other mineralized tissues	885:909	Unlike other mineralized tissues and even coronal dentin, however, the ability of root dentin to dissipate energy during deformation does not decay with age.
34740855	0	40	theme	tooth	10:14	arg1	root					16:19	The stoic tooth root	0:19	The stoic tooth root: how the mineral and extracellular matrix counterbalance to keep aged dentin stable.	0:104	The stoic tooth root: how the mineral and extracellular matrix counterbalance to keep aged dentin stable.
34740855	9	41	theme	collagen	1317:1324	arg1	cross-linking					1326:1338	enzymatic and non-enzymatic collagen cross-linking	1289:1338	enzymatic and non-enzymatic collagen cross-linking	1289:1338	Compositional and ultrastructural analyses revealed higher mineral-to-matrix ratio, altered enzymatic and non-enzymatic collagen cross-linking, increased collagen d-spacing and fibril diameter, and decreased abundance of proteoglycans and sulfation pattern of glycosaminoglycans .
34740855	6	42	theme	root	828:831	arg1	stiffness					848:856	the root dentin overall stiffness	824:856	the root dentin overall stiffness	824:856	We find that the root dentin overall stiffness increases with age.
34740855	4	43	theme	load	518:521	arg1	stresses					523:530	mechanical load stresses	507:530	mechanical load stresses of the tooth-bone system	507:555	Root dentin is integral to tooth function in that it anchors and dissipates mechanical load stresses of the tooth-bone system.
34740855	9	44	theme	proteoglycans	1418:1430	arg1	pattern					1446:1452	sulfation pattern	1436:1452	sulfation pattern of glycosaminoglycans	1436:1474	Compositional and ultrastructural analyses revealed higher mineral-to-matrix ratio, altered enzymatic and non-enzymatic collagen cross-linking, increased collagen d-spacing and fibril diameter, and decreased abundance of proteoglycans and sulfation pattern of glycosaminoglycans .
34740855	9	44	theme	proteoglycans	1418:1430	arg1	abundance					1405:1413	abundance	1405:1413	abundance of proteoglycans	1405:1430	Compositional and ultrastructural analyses revealed higher mineral-to-matrix ratio, altered enzymatic and non-enzymatic collagen cross-linking, increased collagen d-spacing and fibril diameter, and decreased abundance of proteoglycans and sulfation pattern of glycosaminoglycans .
34740855	5	45	theme	X-ray	740:744	arg1	scattering					746:755	small angle X-ray scattering	728:755	small angle X-ray scattering	728:755	Here, we assess the viscoelastic behavior, composition, and ultrastructure of young and old root dentin using nano-dynamic mechanical analysis, micro-Raman spectroscopy, small angle X-ray scattering, atomic force and transmission electron microscopies.
34740855	2	46	theme	physical	307:314	arg1	health					349:354	overall physical, psychological, and social human health	299:354	overall physical, psychological, and social human health	299:354	While resilient, human teeth undergo wear and disease, affecting overall physical, psychological, and social human health.
34740855	9	47	theme	collagen	1351:1358	arg1	d-spacing					1360:1368	increased collagen d-spacing	1341:1368	increased collagen d-spacing	1341:1368	Compositional and ultrastructural analyses revealed higher mineral-to-matrix ratio, altered enzymatic and non-enzymatic collagen cross-linking, increased collagen d-spacing and fibril diameter, and decreased abundance of proteoglycans and sulfation pattern of glycosaminoglycans .
34740855	8	48	theme	deconstruction	1044:1057	arg1	method					1059:1064	a deconstruction method	1042:1064	a deconstruction method to dissect the contribution of mineral and organic matrix	1042:1122	Using a deconstruction method to dissect the contribution of mineral and organic matrix, we find that the damping factor of the organic matrix does deteriorate.
34740855	16	49	theme	biomechanical	2522:2534	arg1	performance					2536:2546	the biomechanical performance	2518:2546	the biomechanical performance of aging dentin with implications for efforts to slow or even reverse the aging process	2518:2634	This strongly suggests that there is a mechanistic link between mineral and matrix components to the biomechanical performance of aging dentin with implications for efforts to slow or even reverse the aging process.
34740855	11	50	theme	matrix	1890:1895	arg1	components					1858:1867	the structural components	1843:1867	the structural components of the extracellular matrix	1843:1895	To explain this discrepancy, we propose that altered matrix-mineral interactions, possibly mediated by carbonate ions sequestered at the mineral interface and/or altered glycosaminoglycans counteract the deleterious effects of aging on the structural components of the extracellular matrix.
34740855	1	51	theme	biosystems	191:200	arg1	function					179:186	function	179:186	function	179:186	Aging is a physiological process with profound impact on the biology and function of biosystems, including the human dentition.
34740855	1	51	theme	biosystems	191:200	arg1	biology					167:173	biology	167:173	biology	167:173	Aging is a physiological process with profound impact on the biology and function of biosystems, including the human dentition.
34740855	11	52	theme	carbonate	1710:1718	arg1	ions					1720:1723	carbonate ions	1710:1723	carbonate ions sequestered at the mineral interface and/or altered glycosaminoglycans	1710:1794	To explain this discrepancy, we propose that altered matrix-mineral interactions, possibly mediated by carbonate ions sequestered at the mineral interface and/or altered glycosaminoglycans counteract the deleterious effects of aging on the structural components of the extracellular matrix.
34740855	1	53	dep	biology	167:173	arg1	the					163:165	the	163:165	the	163:165	Aging is a physiological process with profound impact on the biology and function of biosystems, including the human dentition.
34740855	15	54	theme	older	2243:2247	arg1	dentin					2254:2259	older root dentin	2243:2259	older root dentin	2243:2259	Here, we demonstrate that older root dentin not only has altered mechanical properties, but shows characteristic shifts in mineralization, composition, and post-translational modifications of the matrix.
34740855	10	55	theme	extracellular	1528:1540	arg1	matrix					1542:1547	the extracellular matrix	1524:1547	the extracellular matrix of root dentin	1524:1562	Therefore, even in the absence of remodeling, the extracellular matrix of root dentin shares traits of aging with other tissues.
34740855	2	56	theme	psychological	317:329	arg1	health					349:354	overall physical, psychological, and social human health	299:354	overall physical, psychological, and social human health	299:354	While resilient, human teeth undergo wear and disease, affecting overall physical, psychological, and social human health.
34740855	1	57	theme	human	217:221	arg1	dentition					223:231	the human dentition	213:231	the human dentition	213:231	Aging is a physiological process with profound impact on the biology and function of biosystems, including the human dentition.
34740855	4	58	theme	tooth-bone	539:548	arg1	system					550:555	the tooth-bone system	535:555	the tooth-bone system	535:555	Root dentin is integral to tooth function in that it anchors and dissipates mechanical load stresses of the tooth-bone system.
34740855	10	59	theme	dentin	1557:1562	arg1	matrix					1542:1547	the extracellular matrix	1524:1547	the extracellular matrix of root dentin	1524:1562	Therefore, even in the absence of remodeling, the extracellular matrix of root dentin shares traits of aging with other tissues.
34740855	11	60	theme	matrix-mineral	1660:1673	arg1	interactions					1675:1686	altered matrix-mineral interactions	1652:1686	altered matrix-mineral interactions	1652:1686	To explain this discrepancy, we propose that altered matrix-mineral interactions, possibly mediated by carbonate ions sequestered at the mineral interface and/or altered glycosaminoglycans counteract the deleterious effects of aging on the structural components of the extracellular matrix.
34740855	1	61	theme	profound	144:151	arg1	impact					153:158	profound impact	144:158	profound impact on the biology and function of biosystems, including the human dentition	144:231	Aging is a physiological process with profound impact on the biology and function of biosystems, including the human dentition.
34740855	8	62	theme	matrix	1117:1122	arg1	contribution					1081:1092	the contribution	1077:1092	the contribution of mineral and organic matrix	1077:1122	Using a deconstruction method to dissect the contribution of mineral and organic matrix, we find that the damping factor of the organic matrix does deteriorate.
34740855	6	63	theme	overall	840:846	arg1	stiffness					848:856	the root dentin overall stiffness	824:856	the root dentin overall stiffness	824:856	We find that the root dentin overall stiffness increases with age.
34740855	8	64	theme	organic	1164:1170	arg1	matrix					1172:1177	the organic matrix	1160:1177	the organic matrix	1160:1177	Using a deconstruction method to dissect the contribution of mineral and organic matrix, we find that the damping factor of the organic matrix does deteriorate.
34740855	10	65	theme	other	1592:1596	arg1	tissues					1598:1604	other tissues	1592:1604	other tissues	1592:1604	Therefore, even in the absence of remodeling, the extracellular matrix of root dentin shares traits of aging with other tissues.
34740855	10	66	dep	remodeling	1512:1521	arg1	the					1497:1499	the	1497:1499	the	1497:1499	Therefore, even in the absence of remodeling, the extracellular matrix of root dentin shares traits of aging with other tissues.
34740855	10	66	dep	remodeling	1512:1521	arg1	absence					1501:1507	absence	1501:1507	absence	1501:1507	Therefore, even in the absence of remodeling, the extracellular matrix of root dentin shares traits of aging with other tissues.
34740855	5	67	theme	viscoelastic	578:589	arg1	behavior					591:598	the viscoelastic behavior	574:598	the viscoelastic behavior	574:598	Here, we assess the viscoelastic behavior, composition, and ultrastructure of young and old root dentin using nano-dynamic mechanical analysis, micro-Raman spectroscopy, small angle X-ray scattering, atomic force and transmission electron microscopies.
34740855	9	68	theme	sulfation	1436:1444	arg1	pattern					1446:1452	sulfation pattern	1436:1452	sulfation pattern of glycosaminoglycans	1436:1474	Compositional and ultrastructural analyses revealed higher mineral-to-matrix ratio, altered enzymatic and non-enzymatic collagen cross-linking, increased collagen d-spacing and fibril diameter, and decreased abundance of proteoglycans and sulfation pattern of glycosaminoglycans .
34740855	10	69	theme	aging	1581:1585	arg1	traits					1571:1576	traits	1571:1576	traits of aging	1571:1585	Therefore, even in the absence of remodeling, the extracellular matrix of root dentin shares traits of aging with other tissues.
34740855	5	70	theme	old	646:648	arg1	dentin					655:660	young and old root dentin	636:660	young and old root dentin	636:660	Here, we assess the viscoelastic behavior, composition, and ultrastructure of young and old root dentin using nano-dynamic mechanical analysis, micro-Raman spectroscopy, small angle X-ray scattering, atomic force and transmission electron microscopies.
34740855	5	71	theme	electron	788:795	arg1	microscopies					797:808	transmission electron microscopies	775:808	transmission electron microscopies	775:808	Here, we assess the viscoelastic behavior, composition, and ultrastructure of young and old root dentin using nano-dynamic mechanical analysis, micro-Raman spectroscopy, small angle X-ray scattering, atomic force and transmission electron microscopies.
34740855	12	72	theme	old	1985:1987	arg1	years					1979:1983	65 years old	1976:1987	65 years old	1976:1987	STATEMENT OF SIGNIFICANCE: Globally, a quarter of the population will be over 65 years old by 2050.
34740855	11	73	theme	extracellular	1876:1888	arg1	matrix					1890:1895	the extracellular matrix	1872:1895	the extracellular matrix	1872:1895	To explain this discrepancy, we propose that altered matrix-mineral interactions, possibly mediated by carbonate ions sequestered at the mineral interface and/or altered glycosaminoglycans counteract the deleterious effects of aging on the structural components of the extracellular matrix.
34740855	5	74	theme	dentin	655:660	arg1	composition					601:611	composition	601:611	composition	601:611	Here, we assess the viscoelastic behavior, composition, and ultrastructure of young and old root dentin using nano-dynamic mechanical analysis, micro-Raman spectroscopy, small angle X-ray scattering, atomic force and transmission electron microscopies.
34740855	5	74	theme	dentin	655:660	arg1	ultrastructure					618:631	ultrastructure	618:631	ultrastructure	618:631	Here, we assess the viscoelastic behavior, composition, and ultrastructure of young and old root dentin using nano-dynamic mechanical analysis, micro-Raman spectroscopy, small angle X-ray scattering, atomic force and transmission electron microscopies.
34740855	5	74	theme	dentin	655:660	arg1	behavior					591:598	the viscoelastic behavior	574:598	the viscoelastic behavior	574:598	Here, we assess the viscoelastic behavior, composition, and ultrastructure of young and old root dentin using nano-dynamic mechanical analysis, micro-Raman spectroscopy, small angle X-ray scattering, atomic force and transmission electron microscopies.
34740855	15	75	theme	post-translational	2373:2390	arg1	modifications					2392:2404	post-translational modifications	2373:2404	post-translational modifications of the matrix	2373:2418	Here, we demonstrate that older root dentin not only has altered mechanical properties, but shows characteristic shifts in mineralization, composition, and post-translational modifications of the matrix.
34740855	8	76	theme	damping	1142:1148	arg1	factor					1150:1155	the damping factor	1138:1155	the damping factor of the organic matrix	1138:1177	Using a deconstruction method to dissect the contribution of mineral and organic matrix, we find that the damping factor of the organic matrix does deteriorate.
34740855	15	77	contain	has	2270:2272	arg1	dentin					2254:2259	older root dentin	2243:2259	older root dentin	2243:2259	Here, we demonstrate that older root dentin not only has altered mechanical properties, but shows characteristic shifts in mineralization, composition, and post-translational modifications of the matrix.
34740855	15	77	contain	has	2270:2272	arg2	properties					2293:2302	altered mechanical properties	2274:2302	altered mechanical properties	2274:2302	Here, we demonstrate that older root dentin not only has altered mechanical properties, but shows characteristic shifts in mineralization, composition, and post-translational modifications of the matrix.
34740855	11	78	theme	structural	1847:1856	arg1	components					1858:1867	the structural components	1843:1867	the structural components of the extracellular matrix	1843:1895	To explain this discrepancy, we propose that altered matrix-mineral interactions, possibly mediated by carbonate ions sequestered at the mineral interface and/or altered glycosaminoglycans counteract the deleterious effects of aging on the structural components of the extracellular matrix.
34740855	5	79	theme	young	636:640	arg1	dentin					655:660	young and old root dentin	636:660	young and old root dentin	636:660	Here, we assess the viscoelastic behavior, composition, and ultrastructure of young and old root dentin using nano-dynamic mechanical analysis, micro-Raman spectroscopy, small angle X-ray scattering, atomic force and transmission electron microscopies.
34740855	9	80	theme	Compositional	1197:1209	arg1	analyses					1231:1238	Compositional and ultrastructural analyses	1197:1238	analyses	1231:1238	Compositional and ultrastructural analyses revealed higher mineral-to-matrix ratio, altered enzymatic and non-enzymatic collagen cross-linking, increased collagen d-spacing and fibril diameter, and decreased abundance of proteoglycans and sulfation pattern of glycosaminoglycans .
34740855	7	81	theme	root	960:963	arg1	dentin					965:970	root dentin	960:970	root dentin	960:970	Unlike other mineralized tissues and even coronal dentin, however, the ability of root dentin to dissipate energy during deformation does not decay with age.
34740855	16	82	theme	dentin	2557:2562	arg1	performance					2536:2546	the biomechanical performance	2518:2546	the biomechanical performance of aging dentin with implications for efforts to slow or even reverse the aging process	2518:2634	This strongly suggests that there is a mechanistic link between mineral and matrix components to the biomechanical performance of aging dentin with implications for efforts to slow or even reverse the aging process.
34740855	5	83	theme	micro-Raman	702:712	arg1	spectroscopy					714:725	micro-Raman spectroscopy	702:725	micro-Raman spectroscopy	702:725	Here, we assess the viscoelastic behavior, composition, and ultrastructure of young and old root dentin using nano-dynamic mechanical analysis, micro-Raman spectroscopy, small angle X-ray scattering, atomic force and transmission electron microscopies.
34740855	9	84	theme	ultrastructural	1215:1229	arg1	analyses					1231:1238	Compositional and ultrastructural analyses	1197:1238	analyses	1231:1238	Compositional and ultrastructural analyses revealed higher mineral-to-matrix ratio, altered enzymatic and non-enzymatic collagen cross-linking, increased collagen d-spacing and fibril diameter, and decreased abundance of proteoglycans and sulfation pattern of glycosaminoglycans .
34740855	4	85	theme	tooth	458:462	arg1	function					464:471	tooth function	458:471	tooth function	458:471	Root dentin is integral to tooth function in that it anchors and dissipates mechanical load stresses of the tooth-bone system.
34740855	1	86	with	process	131:137	arg1	impact					153:158	profound impact	144:158	profound impact on the biology and function of biosystems, including the human dentition	144:231	Aging is a physiological process with profound impact on the biology and function of biosystems, including the human dentition.
34740855	1	87	from	impact	153:158	arg1	function					179:186	function	179:186	function	179:186	Aging is a physiological process with profound impact on the biology and function of biosystems, including the human dentition.
34740855	1	87	from	impact	153:158	arg1	biology					167:173	biology	167:173	biology	167:173	Aging is a physiological process with profound impact on the biology and function of biosystems, including the human dentition.
34740855	5	88	theme	nano-dynamic	668:679	arg1	analysis					692:699	nano-dynamic mechanical analysis	668:699	nano-dynamic mechanical analysis	668:699	Here, we assess the viscoelastic behavior, composition, and ultrastructure of young and old root dentin using nano-dynamic mechanical analysis, micro-Raman spectroscopy, small angle X-ray scattering, atomic force and transmission electron microscopies.
34740855	9	89	theme	mineral-to-matrix	1256:1272	arg1	ratio					1274:1278	higher mineral-to-matrix ratio	1249:1278	higher mineral-to-matrix ratio	1249:1278	Compositional and ultrastructural analyses revealed higher mineral-to-matrix ratio, altered enzymatic and non-enzymatic collagen cross-linking, increased collagen d-spacing and fibril diameter, and decreased abundance of proteoglycans and sulfation pattern of glycosaminoglycans .
34740855	11	90	theme	altered	1769:1775	arg1	glycosaminoglycans					1777:1794	altered glycosaminoglycans	1769:1794	altered glycosaminoglycans	1769:1794	To explain this discrepancy, we propose that altered matrix-mineral interactions, possibly mediated by carbonate ions sequestered at the mineral interface and/or altered glycosaminoglycans counteract the deleterious effects of aging on the structural components of the extracellular matrix.
34740855	11	91	theme	deleterious	1811:1821	arg1	effects					1823:1829	the deleterious effects	1807:1829	the deleterious effects of aging on the structural components of the extracellular matrix	1807:1895	To explain this discrepancy, we propose that altered matrix-mineral interactions, possibly mediated by carbonate ions sequestered at the mineral interface and/or altered glycosaminoglycans counteract the deleterious effects of aging on the structural components of the extracellular matrix.
34740855	7	92	theme	coronal	920:926	arg1	dentin					928:933	even coronal dentin	915:933	even coronal dentin	915:933	Unlike other mineralized tissues and even coronal dentin, however, the ability of root dentin to dissipate energy during deformation does not decay with age.
34740855	15	93	theme	characteristic	2315:2328	arg1	shifts					2330:2335	characteristic shifts	2315:2335	characteristic shifts in mineralization, composition, and post-translational modifications of the matrix	2315:2418	Here, we demonstrate that older root dentin not only has altered mechanical properties, but shows characteristic shifts in mineralization, composition, and post-translational modifications of the matrix.
34740855	3	94	theme	aging	401:405	arg1	mechanisms					381:390	the underlying mechanisms	366:390	the underlying mechanisms of tooth aging	366:405	However, the underlying mechanisms of tooth aging remain largely unknown.
34740855	5	95	theme	atomic	758:763	arg1	force					765:769	atomic force	758:769	atomic force	758:769	Here, we assess the viscoelastic behavior, composition, and ultrastructure of young and old root dentin using nano-dynamic mechanical analysis, micro-Raman spectroscopy, small angle X-ray scattering, atomic force and transmission electron microscopies.
34740855	9	96	theme	enzymatic	1289:1297	arg1	cross-linking					1326:1338	enzymatic and non-enzymatic collagen cross-linking	1289:1338	enzymatic and non-enzymatic collagen cross-linking	1289:1338	Compositional and ultrastructural analyses revealed higher mineral-to-matrix ratio, altered enzymatic and non-enzymatic collagen cross-linking, increased collagen d-spacing and fibril diameter, and decreased abundance of proteoglycans and sulfation pattern of glycosaminoglycans .
34740855	15	97	theme	matrix	2413:2418	arg1	mineralization					2340:2353	mineralization	2340:2353	mineralization	2340:2353	Here, we demonstrate that older root dentin not only has altered mechanical properties, but shows characteristic shifts in mineralization, composition, and post-translational modifications of the matrix.
34740855	15	97	theme	matrix	2413:2418	arg1	modifications					2392:2404	post-translational modifications	2373:2404	post-translational modifications of the matrix	2373:2418	Here, we demonstrate that older root dentin not only has altered mechanical properties, but shows characteristic shifts in mineralization, composition, and post-translational modifications of the matrix.
34740855	15	97	theme	matrix	2413:2418	arg1	composition					2356:2366	composition	2356:2366	composition	2356:2366	Here, we demonstrate that older root dentin not only has altered mechanical properties, but shows characteristic shifts in mineralization, composition, and post-translational modifications of the matrix.
34740855	4	98	theme	mechanical	507:516	arg1	stresses					523:530	mechanical load stresses	507:530	mechanical load stresses of the tooth-bone system	507:555	Root dentin is integral to tooth function in that it anchors and dissipates mechanical load stresses of the tooth-bone system.
34740855	2	99	theme	social	336:341	arg1	health					349:354	overall physical, psychological, and social human health	299:354	overall physical, psychological, and social human health	299:354	While resilient, human teeth undergo wear and disease, affecting overall physical, psychological, and social human health.
34740855	9	100	theme	non-enzymatic	1303:1315	arg1	cross-linking					1326:1338	enzymatic and non-enzymatic collagen cross-linking	1289:1338	enzymatic and non-enzymatic collagen cross-linking	1289:1338	Compositional and ultrastructural analyses revealed higher mineral-to-matrix ratio, altered enzymatic and non-enzymatic collagen cross-linking, increased collagen d-spacing and fibril diameter, and decreased abundance of proteoglycans and sulfation pattern of glycosaminoglycans .
34740855	7	101	theme	mineralized	891:901	arg1	tissues					903:909	other mineralized tissues	885:909	other mineralized tissues	885:909	Unlike other mineralized tissues and even coronal dentin, however, the ability of root dentin to dissipate energy during deformation does not decay with age.
34740855	5	102	theme	angle	734:738	arg1	scattering					746:755	small angle X-ray scattering	728:755	small angle X-ray scattering	728:755	Here, we assess the viscoelastic behavior, composition, and ultrastructure of young and old root dentin using nano-dynamic mechanical analysis, micro-Raman spectroscopy, small angle X-ray scattering, atomic force and transmission electron microscopies.
34740855	3	103	theme	underlying	370:379	arg1	mechanisms					381:390	the underlying mechanisms	366:390	the underlying mechanisms of tooth aging	366:405	However, the underlying mechanisms of tooth aging remain largely unknown.
34740855	12	104	theme	population	1952:1961	arg1	quarter					1937:1943	a quarter	1935:1943	a quarter of the population	1935:1961	STATEMENT OF SIGNIFICANCE: Globally, a quarter of the population will be over 65 years old by 2050.
34740855	0	105	theme	mineral	30:36	arg1	counterbalance					63:76	the mineral and extracellular matrix counterbalance	26:76	the mineral and extracellular matrix counterbalance	26:76	The stoic tooth root: how the mineral and extracellular matrix counterbalance to keep aged dentin stable.
34740855	9	106	theme	increased	1341:1349	arg1	d-spacing					1360:1368	increased collagen d-spacing	1341:1368	increased collagen d-spacing	1341:1368	Compositional and ultrastructural analyses revealed higher mineral-to-matrix ratio, altered enzymatic and non-enzymatic collagen cross-linking, increased collagen d-spacing and fibril diameter, and decreased abundance of proteoglycans and sulfation pattern of glycosaminoglycans .
34740855	2	107	theme	overall	299:305	arg1	health					349:354	overall physical, psychological, and social human health	299:354	overall physical, psychological, and social human health	299:354	While resilient, human teeth undergo wear and disease, affecting overall physical, psychological, and social human health.
34740855	16	108	with	performance	2536:2546	arg1	implications					2569:2580	implications	2569:2580	implications for efforts to slow or even reverse the aging process	2569:2634	This strongly suggests that there is a mechanistic link between mineral and matrix components to the biomechanical performance of aging dentin with implications for efforts to slow or even reverse the aging process.
34740855	9	109	theme	fibril	1374:1379	arg1	diameter					1381:1388	fibril diameter	1374:1388	fibril diameter	1374:1388	Compositional and ultrastructural analyses revealed higher mineral-to-matrix ratio, altered enzymatic and non-enzymatic collagen cross-linking, increased collagen d-spacing and fibril diameter, and decreased abundance of proteoglycans and sulfation pattern of glycosaminoglycans .
34740855	0	110	theme	stoic	4:8	arg1	root					16:19	The stoic tooth root	0:19	The stoic tooth root: how the mineral and extracellular matrix counterbalance to keep aged dentin stable.	0:104	The stoic tooth root: how the mineral and extracellular matrix counterbalance to keep aged dentin stable.
34768961	7	0	from	binding	1318:1324	arg1	absence					1351:1357	the absence	1347:1357	the absence of chloride ions	1347:1374	The largest effects on the GAG structures were found for sulphation at position 6, as well as binding of the metal ions in the absence of chloride ions to the carboxylate and sulphate groups, which both increase the GAG conformational flexibility.
34768961	6	1	theme	energy	1101:1106	arg1	landscape					1108:1116	the free energy landscape	1092:1116	the free energy landscape	1092:1116	Different structural parameters were determined from the MD simulations, in combination with a presentation of the free energy landscape of the GAG conformations, which allowed us to unravel the conformational fingerprints unique to each GAG.
34768961	0	2	theme	Concentration	50:62	arg1	Influences					4:13	The Influences	0:13	The Influences of Sulphation, Salt Type, and Salt Concentration on the Structural Heterogeneity of Glycosaminoglycans	0:116	The Influences of Sulphation, Salt Type, and Salt Concentration on the Structural Heterogeneity of Glycosaminoglycans.
34768961	0	3	from	Influences	4:13	arg1	Heterogeneity					82:94	the Structural Heterogeneity	67:94	the Structural Heterogeneity of Glycosaminoglycans	67:116	The Influences of Sulphation, Salt Type, and Salt Concentration on the Structural Heterogeneity of Glycosaminoglycans.
34768961	7	4	theme	carboxylate	1383:1393	arg1	groups					1408:1413	the carboxylate and sulphate groups	1379:1413	groups	1408:1413	The largest effects on the GAG structures were found for sulphation at position 6, as well as binding of the metal ions in the absence of chloride ions to the carboxylate and sulphate groups, which both increase the GAG conformational flexibility.
34768961	1	5	theme	biochemical	153:163	arg1	importance					165:174	the biochemical importance	149:174	the biochemical importance of glycosaminoglycans (GAGs)	149:203	The increasing recognition of the biochemical importance of glycosaminoglycans (GAGs) has in recent times made them the center of attention of recent research investigations.
34768961	6	6	theme	structural	991:1000	arg1	parameters					1002:1011	Different structural parameters	981:1011	Different structural parameters	981:1011	Different structural parameters were determined from the MD simulations, in combination with a presentation of the free energy landscape of the GAG conformations, which allowed us to unravel the conformational fingerprints unique to each GAG.
34768961	0	7	theme	Structural	71:80	arg1	Heterogeneity					82:94	the Structural Heterogeneity	67:94	the Structural Heterogeneity of Glycosaminoglycans	67:116	The Influences of Sulphation, Salt Type, and Salt Concentration on the Structural Heterogeneity of Glycosaminoglycans.
34768961	7	8	theme	GAG	1440:1442	arg1	flexibility					1459:1469	the GAG conformational flexibility	1436:1469	the GAG conformational flexibility	1436:1469	The largest effects on the GAG structures were found for sulphation at position 6, as well as binding of the metal ions in the absence of chloride ions to the carboxylate and sulphate groups, which both increase the GAG conformational flexibility.
34768961	4	9	theme	substitution	663:674	arg1	patterns					676:683	different substitution patterns	653:683	different substitution patterns	653:683	Four major GAGs with different substitution patterns, namely hyaluronic acid as unsulphated GAG, heparan-6-sulphate, chondroitin-4-sulphate, and chondroitin-6-sulphate, were investigated to elucidate the influence of sulphation on the dynamical features of GAGs.
34768961	7	10	theme	sulphate	1399:1406	arg1	groups					1408:1413	the carboxylate and sulphate groups	1379:1413	groups	1408:1413	The largest effects on the GAG structures were found for sulphation at position 6, as well as binding of the metal ions in the absence of chloride ions to the carboxylate and sulphate groups, which both increase the GAG conformational flexibility.
34768961	7	11	theme	ions	1371:1374	arg1	absence					1351:1357	the absence	1347:1357	the absence of chloride ions	1347:1374	The largest effects on the GAG structures were found for sulphation at position 6, as well as binding of the metal ions in the absence of chloride ions to the carboxylate and sulphate groups, which both increase the GAG conformational flexibility.
34768961	4	12	theme	GAGs	889:892	arg1	features					877:884	the dynamical features	863:884	the dynamical features of GAGs	863:892	Four major GAGs with different substitution patterns, namely hyaluronic acid as unsulphated GAG, heparan-6-sulphate, chondroitin-4-sulphate, and chondroitin-6-sulphate, were investigated to elucidate the influence of sulphation on the dynamical features of GAGs.
34768961	6	13	theme	conformational	1176:1189	arg1	fingerprints					1191:1202	the conformational fingerprints	1172:1202	the conformational fingerprints unique to each GAG	1172:1221	Different structural parameters were determined from the MD simulations, in combination with a presentation of the free energy landscape of the GAG conformations, which allowed us to unravel the conformational fingerprints unique to each GAG.
34768961	1	14	theme	attention	249:257	arg1	center					239:244	the center	235:244	them the center of attention of recent research investigations	230:291	The increasing recognition of the biochemical importance of glycosaminoglycans (GAGs) has in recent times made them the center of attention of recent research investigations.
34768961	7	15	theme	GAG	1251:1253	arg1	structures					1255:1264	the GAG structures	1247:1264	the GAG structures	1247:1264	The largest effects on the GAG structures were found for sulphation at position 6, as well as binding of the metal ions in the absence of chloride ions to the carboxylate and sulphate groups, which both increase the GAG conformational flexibility.
34768961	6	16	theme	conformations	1129:1141	arg1	landscape					1108:1116	the free energy landscape	1092:1116	the free energy landscape	1092:1116	Different structural parameters were determined from the MD simulations, in combination with a presentation of the free energy landscape of the GAG conformations, which allowed us to unravel the conformational fingerprints unique to each GAG.
34768961	4	17	theme	different	653:661	arg1	patterns					676:683	different substitution patterns	653:683	different substitution patterns	653:683	Four major GAGs with different substitution patterns, namely hyaluronic acid as unsulphated GAG, heparan-6-sulphate, chondroitin-4-sulphate, and chondroitin-6-sulphate, were investigated to elucidate the influence of sulphation on the dynamical features of GAGs.
34768961	1	18	theme	importance	165:174	arg1	recognition					134:144	The increasing recognition	119:144	The increasing recognition of the biochemical importance of glycosaminoglycans (GAGs)	119:203	The increasing recognition of the biochemical importance of glycosaminoglycans (GAGs) has in recent times made them the center of attention of recent research investigations.
34768961	2	19	theme	activity	453:460	arg1	composition					423:433	the chemical composition	410:433	the chemical composition of GAGs and their activity	410:460	It became evident that subtle conformational factors play an important role in determining the relationship between the chemical composition of GAGs and their activity.
34768961	7	20	located	found	1271:1275	arg2	effects					1236:1242	The largest effects	1224:1242	The largest effects on the GAG structures	1224:1264	The largest effects on the GAG structures were found for sulphation at position 6, as well as binding of the metal ions in the absence of chloride ions to the carboxylate and sulphate groups, which both increase the GAG conformational flexibility.
34768961	7	20	located	found	1271:1275	arg1	position					1295:1302	position 6	1295:1304	position 6	1295:1304	The largest effects on the GAG structures were found for sulphation at position 6, as well as binding of the metal ions in the absence of chloride ions to the carboxylate and sulphate groups, which both increase the GAG conformational flexibility.
34768961	4	21	theme	sulphation	849:858	arg1	influence					836:844	the influence	832:844	the influence of sulphation on the dynamical features of GAGs	832:892	Four major GAGs with different substitution patterns, namely hyaluronic acid as unsulphated GAG, heparan-6-sulphate, chondroitin-4-sulphate, and chondroitin-6-sulphate, were investigated to elucidate the influence of sulphation on the dynamical features of GAGs.
34768961	6	22	theme	MD	1038:1039	arg1	simulations					1041:1051	the MD simulations	1034:1051	the MD simulations	1034:1051	Different structural parameters were determined from the MD simulations, in combination with a presentation of the free energy landscape of the GAG conformations, which allowed us to unravel the conformational fingerprints unique to each GAG.
34768961	6	23	theme	free	1096:1099	arg1	landscape					1108:1116	the free energy landscape	1092:1116	the free energy landscape	1092:1116	Different structural parameters were determined from the MD simulations, in combination with a presentation of the free energy landscape of the GAG conformations, which allowed us to unravel the conformational fingerprints unique to each GAG.
34768961	4	24	theme	major	637:641	arg1	GAGs					643:646	Four major GAGs	632:646	Four major GAGs with different substitution patterns	632:683	Four major GAGs with different substitution patterns, namely hyaluronic acid as unsulphated GAG, heparan-6-sulphate, chondroitin-4-sulphate, and chondroitin-6-sulphate, were investigated to elucidate the influence of sulphation on the dynamical features of GAGs.
34768961	4	24	theme	major	637:641	arg1	acid					704:707	hyaluronic acid	693:707	hyaluronic acid as unsulphated GAG, heparan-6-sulphate, chondroitin-4-sulphate, and chondroitin-6-sulphate	693:798	Four major GAGs with different substitution patterns, namely hyaluronic acid as unsulphated GAG, heparan-6-sulphate, chondroitin-4-sulphate, and chondroitin-6-sulphate, were investigated to elucidate the influence of sulphation on the dynamical features of GAGs.
34768961	4	25	theme	dynamical	867:875	arg1	features					877:884	the dynamical features	863:884	the dynamical features of GAGs	863:892	Four major GAGs with different substitution patterns, namely hyaluronic acid as unsulphated GAG, heparan-6-sulphate, chondroitin-4-sulphate, and chondroitin-6-sulphate, were investigated to elucidate the influence of sulphation on the dynamical features of GAGs.
34768961	7	26	theme	largest	1228:1234	arg1	effects					1236:1242	The largest effects	1224:1242	The largest effects on the GAG structures	1224:1264	The largest effects on the GAG structures were found for sulphation at position 6, as well as binding of the metal ions in the absence of chloride ions to the carboxylate and sulphate groups, which both increase the GAG conformational flexibility.
34768961	5	27	theme	KCl	940:942	arg1	concentrations					944:957	NaCl and KCl concentrations	931:957	NaCl and KCl concentrations	931:957	Moreover, the effects of increasing NaCl and KCl concentrations were studied as well.
34768961	7	28	from	ions	1339:1342	arg1	absence					1351:1357	the absence	1347:1357	the absence of chloride ions	1347:1374	The largest effects on the GAG structures were found for sulphation at position 6, as well as binding of the metal ions in the absence of chloride ions to the carboxylate and sulphate groups, which both increase the GAG conformational flexibility.
34768961	1	29	theme	glycosaminoglycans	179:196	arg1	importance					165:174	the biochemical importance	149:174	the biochemical importance of glycosaminoglycans (GAGs)	149:203	The increasing recognition of the biochemical importance of glycosaminoglycans (GAGs) has in recent times made them the center of attention of recent research investigations.
34768961	6	30	theme	Different	981:989	arg1	parameters					1002:1011	Different structural parameters	981:1011	Different structural parameters	981:1011	Different structural parameters were determined from the MD simulations, in combination with a presentation of the free energy landscape of the GAG conformations, which allowed us to unravel the conformational fingerprints unique to each GAG.
34768961	6	31	with	combination	1057:1067	arg1	presentation					1076:1087	a presentation	1074:1087	a presentation of the free energy landscape of the GAG conformations, which allowed us to unravel the conformational fingerprints unique to each GAG	1074:1221	Different structural parameters were determined from the MD simulations, in combination with a presentation of the free energy landscape of the GAG conformations, which allowed us to unravel the conformational fingerprints unique to each GAG.
34768961	2	32	theme	GAGs	438:441	arg1	composition					423:433	the chemical composition	410:433	the chemical composition of GAGs and their activity	410:460	It became evident that subtle conformational factors play an important role in determining the relationship between the chemical composition of GAGs and their activity.
34768961	1	33	theme	recent	262:267	arg1	investigations					278:291	recent research investigations	262:291	recent research investigations	262:291	The increasing recognition of the biochemical importance of glycosaminoglycans (GAGs) has in recent times made them the center of attention of recent research investigations.
34768961	0	34	gly	Heterogeneity	82:94	arg1	Glycosaminoglycans					99:116	Glycosaminoglycans	99:116	Glycosaminoglycans	99:116	The Influences of Sulphation, Salt Type, and Salt Concentration on the Structural Heterogeneity of Glycosaminoglycans.
34768961	1	35	theme	research	269:276	arg1	investigations					278:291	recent research investigations	262:291	recent research investigations	262:291	The increasing recognition of the biochemical importance of glycosaminoglycans (GAGs) has in recent times made them the center of attention of recent research investigations.
34768961	0	36	theme	Glycosaminoglycans	99:116	arg1	Heterogeneity					82:94	the Structural Heterogeneity	67:94	the Structural Heterogeneity of Glycosaminoglycans	67:116	The Influences of Sulphation, Salt Type, and Salt Concentration on the Structural Heterogeneity of Glycosaminoglycans.
34768961	3	37	theme	structural	508:517	arg1	flexibility					519:529	their structural flexibility	502:529	their structural flexibility	502:529	Therefore, a thorough understanding of their structural flexibility is needed, which is addressed in this work by means of all-atom molecular dynamics (MD) simulations.
34768961	7	38	from	absence	1351:1357	arg1	binding					1318:1324	binding	1318:1324	binding of the metal ions in the absence of chloride ions to the carboxylate and sulphate groups, which both increase the GAG conformational flexibility	1318:1469	The largest effects on the GAG structures were found for sulphation at position 6, as well as binding of the metal ions in the absence of chloride ions to the carboxylate and sulphate groups, which both increase the GAG conformational flexibility.
34768961	2	39	theme	chemical	414:421	arg1	composition					423:433	the chemical composition	410:433	the chemical composition of GAGs and their activity	410:460	It became evident that subtle conformational factors play an important role in determining the relationship between the chemical composition of GAGs and their activity.
34768961	0	40	theme	Salt	30:33	arg1	Type					35:38	Salt Type	30:38	Salt Type	30:38	The Influences of Sulphation, Salt Type, and Salt Concentration on the Structural Heterogeneity of Glycosaminoglycans.
34768961	1	41	theme	investigations	278:291	arg1	attention					249:257	attention	249:257	attention of recent research investigations	249:291	The increasing recognition of the biochemical importance of glycosaminoglycans (GAGs) has in recent times made them the center of attention of recent research investigations.
34768961	1	42	dep	them	230:233	arg1	center					239:244	the center	235:244	them the center of attention of recent research investigations	230:291	The increasing recognition of the biochemical importance of glycosaminoglycans (GAGs) has in recent times made them the center of attention of recent research investigations.
34768961	6	43	theme	GAG	1125:1127	arg1	conformations					1129:1141	the GAG conformations	1121:1141	the GAG conformations	1121:1141	Different structural parameters were determined from the MD simulations, in combination with a presentation of the free energy landscape of the GAG conformations, which allowed us to unravel the conformational fingerprints unique to each GAG.
34768961	4	44	with	GAGs	643:646	arg1	patterns					676:683	different substitution patterns	653:683	different substitution patterns	653:683	Four major GAGs with different substitution patterns, namely hyaluronic acid as unsulphated GAG, heparan-6-sulphate, chondroitin-4-sulphate, and chondroitin-6-sulphate, were investigated to elucidate the influence of sulphation on the dynamical features of GAGs.
34768961	0	45	theme	Sulphation	18:27	arg1	Influences					4:13	The Influences	0:13	The Influences of Sulphation, Salt Type, and Salt Concentration on the Structural Heterogeneity of Glycosaminoglycans	0:116	The Influences of Sulphation, Salt Type, and Salt Concentration on the Structural Heterogeneity of Glycosaminoglycans.
34768961	7	46	dep	increase	1427:1434	arg1	both					1422:1425	both	1422:1425	both	1422:1425	The largest effects on the GAG structures were found for sulphation at position 6, as well as binding of the metal ions in the absence of chloride ions to the carboxylate and sulphate groups, which both increase the GAG conformational flexibility.
34768961	4	47	from	influence	836:844	arg1	features					877:884	the dynamical features	863:884	the dynamical features of GAGs	863:892	Four major GAGs with different substitution patterns, namely hyaluronic acid as unsulphated GAG, heparan-6-sulphate, chondroitin-4-sulphate, and chondroitin-6-sulphate, were investigated to elucidate the influence of sulphation on the dynamical features of GAGs.
34768961	0	48	theme	Type	35:38	arg1	Influences					4:13	The Influences	0:13	The Influences of Sulphation, Salt Type, and Salt Concentration on the Structural Heterogeneity of Glycosaminoglycans	0:116	The Influences of Sulphation, Salt Type, and Salt Concentration on the Structural Heterogeneity of Glycosaminoglycans.
34768961	2	49	theme	conformational	324:337	arg1	factors					339:345	subtle conformational factors	317:345	subtle conformational factors	317:345	It became evident that subtle conformational factors play an important role in determining the relationship between the chemical composition of GAGs and their activity.
34768961	6	50	theme	unique	1204:1209	arg1	fingerprints					1191:1202	the conformational fingerprints	1172:1202	the conformational fingerprints unique to each GAG	1172:1221	Different structural parameters were determined from the MD simulations, in combination with a presentation of the free energy landscape of the GAG conformations, which allowed us to unravel the conformational fingerprints unique to each GAG.
34768961	7	51	from	effects	1236:1242	arg1	structures					1255:1264	the GAG structures	1247:1264	the GAG structures	1247:1264	The largest effects on the GAG structures were found for sulphation at position 6, as well as binding of the metal ions in the absence of chloride ions to the carboxylate and sulphate groups, which both increase the GAG conformational flexibility.
34768961	7	52	theme	metal	1333:1337	arg1	ions					1339:1342	the metal ions	1329:1342	the metal ions in the absence of chloride ions	1329:1374	The largest effects on the GAG structures were found for sulphation at position 6, as well as binding of the metal ions in the absence of chloride ions to the carboxylate and sulphate groups, which both increase the GAG conformational flexibility.
34768961	3	53	theme	all-atom	586:593	arg1	dynamics					605:612	all-atom molecular dynamics	586:612	all-atom molecular dynamics (MD) simulations	586:629	Therefore, a thorough understanding of their structural flexibility is needed, which is addressed in this work by means of all-atom molecular dynamics (MD) simulations.
34768961	3	53	theme	all-atom	586:593	arg1	MD					615:616	MD	615:616	MD	615:616	Therefore, a thorough understanding of their structural flexibility is needed, which is addressed in this work by means of all-atom molecular dynamics (MD) simulations.
34768961	2	54	theme	subtle	317:322	arg1	factors					339:345	subtle conformational factors	317:345	subtle conformational factors	317:345	It became evident that subtle conformational factors play an important role in determining the relationship between the chemical composition of GAGs and their activity.
34768961	7	55	theme	chloride	1362:1369	arg1	ions					1371:1374	chloride ions	1362:1374	chloride ions	1362:1374	The largest effects on the GAG structures were found for sulphation at position 6, as well as binding of the metal ions in the absence of chloride ions to the carboxylate and sulphate groups, which both increase the GAG conformational flexibility.
34768961	1	56	theme	recent	212:217	arg1	times					219:223	recent times	212:223	recent times made them the center of attention of recent research investigations	212:291	The increasing recognition of the biochemical importance of glycosaminoglycans (GAGs) has in recent times made them the center of attention of recent research investigations.
34768961	5	57	theme	NaCl	931:934	arg1	concentrations					944:957	NaCl and KCl concentrations	931:957	NaCl and KCl concentrations	931:957	Moreover, the effects of increasing NaCl and KCl concentrations were studied as well.
34768961	7	58	theme	ions	1339:1342	arg1	binding					1318:1324	binding	1318:1324	binding of the metal ions in the absence of chloride ions to the carboxylate and sulphate groups, which both increase the GAG conformational flexibility	1318:1469	The largest effects on the GAG structures were found for sulphation at position 6, as well as binding of the metal ions in the absence of chloride ions to the carboxylate and sulphate groups, which both increase the GAG conformational flexibility.
34768961	1	59	theme	increasing	123:132	arg1	recognition					134:144	The increasing recognition	119:144	The increasing recognition of the biochemical importance of glycosaminoglycans (GAGs)	119:203	The increasing recognition of the biochemical importance of glycosaminoglycans (GAGs) has in recent times made them the center of attention of recent research investigations.
34768961	4	60	theme	unsulphated	712:722	arg1	GAG					724:726	unsulphated GAG	712:726	unsulphated GAG	712:726	Four major GAGs with different substitution patterns, namely hyaluronic acid as unsulphated GAG, heparan-6-sulphate, chondroitin-4-sulphate, and chondroitin-6-sulphate, were investigated to elucidate the influence of sulphation on the dynamical features of GAGs.
34768961	0	61	theme	Salt	45:48	arg1	Concentration					50:62	Salt Concentration	45:62	Salt Concentration	45:62	The Influences of Sulphation, Salt Type, and Salt Concentration on the Structural Heterogeneity of Glycosaminoglycans.
34768961	6	62	theme	landscape	1108:1116	arg1	presentation					1076:1087	a presentation	1074:1087	a presentation of the free energy landscape of the GAG conformations, which allowed us to unravel the conformational fingerprints unique to each GAG	1074:1221	Different structural parameters were determined from the MD simulations, in combination with a presentation of the free energy landscape of the GAG conformations, which allowed us to unravel the conformational fingerprints unique to each GAG.
34768961	3	63	theme	molecular	595:603	arg1	dynamics					605:612	all-atom molecular dynamics	586:612	all-atom molecular dynamics (MD) simulations	586:629	Therefore, a thorough understanding of their structural flexibility is needed, which is addressed in this work by means of all-atom molecular dynamics (MD) simulations.
34768961	3	63	theme	molecular	595:603	arg1	MD					615:616	MD	615:616	MD	615:616	Therefore, a thorough understanding of their structural flexibility is needed, which is addressed in this work by means of all-atom molecular dynamics (MD) simulations.
34768961	3	64	theme	flexibility	519:529	arg1	understanding					485:497	a thorough understanding	474:497	a thorough understanding of their structural flexibility	474:529	Therefore, a thorough understanding of their structural flexibility is needed, which is addressed in this work by means of all-atom molecular dynamics (MD) simulations.
34768961	3	65	theme	thorough	476:483	arg1	understanding					485:497	a thorough understanding	474:497	a thorough understanding of their structural flexibility	474:529	Therefore, a thorough understanding of their structural flexibility is needed, which is addressed in this work by means of all-atom molecular dynamics (MD) simulations.
34768961	7	66	theme	conformational	1444:1457	arg1	flexibility					1459:1469	the GAG conformational flexibility	1436:1469	the GAG conformational flexibility	1436:1469	The largest effects on the GAG structures were found for sulphation at position 6, as well as binding of the metal ions in the absence of chloride ions to the carboxylate and sulphate groups, which both increase the GAG conformational flexibility.
34768961	3	67	theme	dynamics	605:612	arg1	simulations					619:629	all-atom molecular dynamics (MD) simulations	586:629	all-atom molecular dynamics (MD) simulations	586:629	Therefore, a thorough understanding of their structural flexibility is needed, which is addressed in this work by means of all-atom molecular dynamics (MD) simulations.
34768961	2	68	theme	important	355:363	arg1	role					365:368	an important role	352:368	an important role	352:368	It became evident that subtle conformational factors play an important role in determining the relationship between the chemical composition of GAGs and their activity.
34768961	4	69	theme	hyaluronic	693:702	arg1	acid					704:707	hyaluronic acid	693:707	hyaluronic acid as unsulphated GAG, heparan-6-sulphate, chondroitin-4-sulphate, and chondroitin-6-sulphate	693:798	Four major GAGs with different substitution patterns, namely hyaluronic acid as unsulphated GAG, heparan-6-sulphate, chondroitin-4-sulphate, and chondroitin-6-sulphate, were investigated to elucidate the influence of sulphation on the dynamical features of GAGs.
34768961	4	69	theme	hyaluronic	693:702	arg1	GAGs					643:646	Four major GAGs	632:646	Four major GAGs with different substitution patterns	632:683	Four major GAGs with different substitution patterns, namely hyaluronic acid as unsulphated GAG, heparan-6-sulphate, chondroitin-4-sulphate, and chondroitin-6-sulphate, were investigated to elucidate the influence of sulphation on the dynamical features of GAGs.
32985092	0	0	theme	composite	74:82	arg1	hydrogels					84:92	sulfate composite hydrogels	66:92	sulfate composite hydrogels containing strontium-doped hydroxyapatite as promising biomaterials	66:160	Synthesis and characterization of poly(vinyl alcohol)/chondroitin sulfate composite hydrogels containing strontium-doped hydroxyapatite as promising biomaterials.
32985092	1	1	theme	freeze/thaw	323:333	arg1	method					335:340	a freeze/thaw method	321:340	a freeze/thaw method	321:340	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
32985092	3	2	theme	swelling	783:790	arg1	degree					792:797	swelling degree	783:797	swelling degree	783:797	Physical-chemical characterizations of particles and hydrogels included scanning electron microscopy, energy-dispersive spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetry, porosity, compressive strength/elastic modulus, swelling degree, and cell viability.
32985092	10	3	theme	viability	1302:1310	arg1	essay					1312:1316	cell viability essay	1297:1316	cell viability essay	1297:1316	In cell viability essay, increased cell growth (above 120%) was observed in all groups including the control hydrogel, suggesting a bioactive effect.
32985092	11	4	theme	promising	1549:1557	arg1	properties					1599:1608	promising morphological, mechanical, and swelling properties	1549:1608	promising morphological, mechanical, and swelling properties	1549:1608	In conclusion, PVA/CS hydrogels containing HA or HASr particles were successfully synthesized and showed promising morphological, mechanical, and swelling properties, which are particularly required for scaffolding.
32985092	0	5	theme	sulfate	66:72	arg1	hydrogels					84:92	sulfate composite hydrogels	66:92	sulfate composite hydrogels containing strontium-doped hydroxyapatite as promising biomaterials	66:160	Synthesis and characterization of poly(vinyl alcohol)/chondroitin sulfate composite hydrogels containing strontium-doped hydroxyapatite as promising biomaterials.
32985092	9	6	theme	water	1216:1220	arg1	uptake					1222:1227	a faster water uptake	1207:1227	a faster water uptake	1207:1227	HASr particles led to a faster water uptake but did not interfere with the total hydrogel swelling capacity.
32985092	6	7	theme	high	1019:1022	arg1	porosity					1024:1031	high porosity	1019:1031	high porosity	1019:1031	All hydrogels exhibited high porosity.
32985092	11	8	theme	mechanical	1574:1583	arg1	properties					1599:1608	promising morphological, mechanical, and swelling properties	1549:1608	promising morphological, mechanical, and swelling properties	1549:1608	In conclusion, PVA/CS hydrogels containing HA or HASr particles were successfully synthesized and showed promising morphological, mechanical, and swelling properties, which are particularly required for scaffolding.
32985092	1	9	theme	vinyl	174:178	arg1	PVA/CS					210:215	PVA/CS	210:215	PVA/CS	210:215	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
32985092	1	9	theme	vinyl	174:178	arg1	sulfate					201:207	Novel poly(vinyl alcohol)/chondroitin sulfate	163:207	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles	163:299	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
32985092	10	10	theme	cell	1297:1300	arg1	essay					1312:1316	cell viability essay	1297:1316	cell viability essay	1297:1316	In cell viability essay, increased cell growth (above 120%) was observed in all groups including the control hydrogel, suggesting a bioactive effect.
32985092	9	11	theme	hydrogel	1266:1273	arg1	capacity					1284:1291	the total hydrogel swelling capacity	1256:1291	the total hydrogel swelling capacity	1256:1291	HASr particles led to a faster water uptake but did not interfere with the total hydrogel swelling capacity.
32985092	9	12	theme	swelling	1275:1282	arg1	capacity					1284:1291	the total hydrogel swelling capacity	1256:1291	the total hydrogel swelling capacity	1256:1291	HASr particles led to a faster water uptake but did not interfere with the total hydrogel swelling capacity.
32985092	3	13	theme	compressive	745:755	arg1	modulus					774:780	compressive strength/elastic modulus	745:780	compressive strength/elastic modulus	745:780	Physical-chemical characterizations of particles and hydrogels included scanning electron microscopy, energy-dispersive spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetry, porosity, compressive strength/elastic modulus, swelling degree, and cell viability.
32985092	8	14	theme	mechanical	1107:1116	arg1	strength					1118:1125	The mechanical strength	1103:1125	The mechanical strength	1103:1125	The mechanical strength was improved as the fraction of HA or HASr was increased.
32985092	3	15	theme	electron	604:611	arg1	microscopy					613:622	scanning electron microscopy	595:622	scanning electron microscopy	595:622	Physical-chemical characterizations of particles and hydrogels included scanning electron microscopy, energy-dispersive spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetry, porosity, compressive strength/elastic modulus, swelling degree, and cell viability.
32985092	4	16	from	shape	848:852	arg1	irregular					835:843	irregular	835:843	irregular	835:843	Particles were irregular in shape and appeared to have narrow size variation.
32985092	4	17	contain	have	870:873	arg2	variation					887:895	narrow size variation	875:895	narrow size variation	875:895	Particles were irregular in shape and appeared to have narrow size variation.
32985092	4	17	contain	have	870:873	arg1	Particles					820:828	Particles	820:828	Particles	820:828	Particles were irregular in shape and appeared to have narrow size variation.
32985092	9	18	theme	HASr	1185:1188	arg1	particles					1190:1198	HASr particles	1185:1198	HASr particles	1185:1198	HASr particles led to a faster water uptake but did not interfere with the total hydrogel swelling capacity.
32985092	4	19	from	irregular	835:843	arg1	shape					848:852	shape	848:852	shape	848:852	Particles were irregular in shape and appeared to have narrow size variation.
32985092	3	20	theme	particles	562:570	arg1	characterizations					541:557	Physical-chemical characterizations	523:557	Physical-chemical characterizations of particles and hydrogels	523:584	Physical-chemical characterizations of particles and hydrogels included scanning electron microscopy, energy-dispersive spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetry, porosity, compressive strength/elastic modulus, swelling degree, and cell viability.
32985092	11	21	dep	HA	1487:1488	arg1	particles					1498:1506	particles	1498:1506	particles	1498:1506	In conclusion, PVA/CS hydrogels containing HA or HASr particles were successfully synthesized and showed promising morphological, mechanical, and swelling properties, which are particularly required for scaffolding.
32985092	1	22	theme	Sr-doped	272:279	arg1	particles					291:299	Sr-doped HA (HASr) particles	272:299	Sr-doped HA (HASr) particles	272:299	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
32985092	10	23	theme	control	1395:1401	arg1	hydrogel					1403:1410	the control hydrogel	1391:1410	the control hydrogel	1391:1410	In cell viability essay, increased cell growth (above 120%) was observed in all groups including the control hydrogel, suggesting a bioactive effect.
32985092	3	24	theme	Physical-chemical	523:539	arg1	characterizations					541:557	Physical-chemical characterizations	523:557	Physical-chemical characterizations of particles and hydrogels	523:584	Physical-chemical characterizations of particles and hydrogels included scanning electron microscopy, energy-dispersive spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetry, porosity, compressive strength/elastic modulus, swelling degree, and cell viability.
32985092	5	25	theme	hydrogels	932:940	arg1	behavior					910:917	The thermal behavior	898:917	The thermal behavior of composite hydrogels	898:940	The thermal behavior of composite hydrogels was altered compared to the control (bare) hydrogel.
32985092	1	26	theme	alcohol	180:186	arg1	PVA/CS					210:215	PVA/CS	210:215	PVA/CS	210:215	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
32985092	1	26	theme	alcohol	180:186	arg1	sulfate					201:207	Novel poly(vinyl alcohol)/chondroitin sulfate	163:207	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles	163:299	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
32985092	0	27	theme	strontium-doped	105:119	arg1	biomaterials					149:160	promising biomaterials	139:160	promising biomaterials	139:160	Synthesis and characterization of poly(vinyl alcohol)/chondroitin sulfate composite hydrogels containing strontium-doped hydroxyapatite as promising biomaterials.
32985092	0	27	theme	strontium-doped	105:119	arg1	hydroxyapatite					121:134	strontium-doped hydroxyapatite	105:134	strontium-doped hydroxyapatite	105:134	Synthesis and characterization of poly(vinyl alcohol)/chondroitin sulfate composite hydrogels containing strontium-doped hydroxyapatite as promising biomaterials.
32985092	10	28	theme	increased	1319:1327	arg1	growth					1334:1339	increased cell growth	1319:1339	increased cell growth (above 120%)	1319:1352	In cell viability essay, increased cell growth (above 120%) was observed in all groups including the control hydrogel, suggesting a bioactive effect.
32985092	10	28	theme	increased	1319:1327	arg1	%					1351:1351	above 120%	1342:1351	above 120%	1342:1351	In cell viability essay, increased cell growth (above 120%) was observed in all groups including the control hydrogel, suggesting a bioactive effect.
32985092	5	29	theme	composite	922:930	arg1	hydrogels					932:940	composite hydrogels	922:940	composite hydrogels	922:940	The thermal behavior of composite hydrogels was altered compared to the control (bare) hydrogel.
32985092	1	30	theme	biomedical	375:384	arg1	applications					386:397	biomedical applications	375:397	biomedical applications	375:397	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
32985092	1	31	theme	/chondroitin	188:199	arg1	PVA/CS					210:215	PVA/CS	210:215	PVA/CS	210:215	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
32985092	1	31	theme	/chondroitin	188:199	arg1	sulfate					201:207	Novel poly(vinyl alcohol)/chondroitin sulfate	163:207	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles	163:299	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
32985092	1	32	theme	HA	281:282	arg1	particles					291:299	Sr-doped HA (HASr) particles	272:299	Sr-doped HA (HASr) particles	272:299	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
32985092	10	33	theme	bioactive	1426:1434	arg1	effect					1436:1441	a bioactive effect	1424:1441	a bioactive effect	1424:1441	In cell viability essay, increased cell growth (above 120%) was observed in all groups including the control hydrogel, suggesting a bioactive effect.
32985092	3	34	theme	scanning	595:602	arg1	microscopy					613:622	scanning electron microscopy	595:622	scanning electron microscopy	595:622	Physical-chemical characterizations of particles and hydrogels included scanning electron microscopy, energy-dispersive spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetry, porosity, compressive strength/elastic modulus, swelling degree, and cell viability.
32985092	1	35	theme	sulfate	201:207	arg1	hydrogels					228:236	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels	163:236	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles	163:299	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
32985092	2	36	theme	wet-precipitation	434:450	arg1	method					452:457	a wet-precipitation method	432:457	a wet-precipitation method	432:457	HA and HASr were synthesized by a wet-precipitation method and added to the composite hydrogels in fractions up to 15 wt%.
32985092	10	37	theme	cell	1329:1332	arg1	growth					1334:1339	increased cell growth	1319:1339	increased cell growth (above 120%)	1319:1352	In cell viability essay, increased cell growth (above 120%) was observed in all groups including the control hydrogel, suggesting a bioactive effect.
32985092	10	37	theme	cell	1329:1332	arg1	%					1351:1351	above 120%	1342:1351	above 120%	1342:1351	In cell viability essay, increased cell growth (above 120%) was observed in all groups including the control hydrogel, suggesting a bioactive effect.
32985092	0	38	theme	promising	139:147	arg1	biomaterials					149:160	promising biomaterials	139:160	promising biomaterials	139:160	Synthesis and characterization of poly(vinyl alcohol)/chondroitin sulfate composite hydrogels containing strontium-doped hydroxyapatite as promising biomaterials.
32985092	0	38	theme	promising	139:147	arg1	hydroxyapatite					121:134	strontium-doped hydroxyapatite	105:134	strontium-doped hydroxyapatite	105:134	Synthesis and characterization of poly(vinyl alcohol)/chondroitin sulfate composite hydrogels containing strontium-doped hydroxyapatite as promising biomaterials.
32985092	9	39	theme	total	1260:1264	arg1	capacity					1284:1291	the total hydrogel swelling capacity	1256:1291	the total hydrogel swelling capacity	1256:1291	HASr particles led to a faster water uptake but did not interfere with the total hydrogel swelling capacity.
32985092	1	40	theme	HASr	285:288	arg1	particles					291:299	Sr-doped HA (HASr) particles	272:299	Sr-doped HA (HASr) particles	272:299	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
32985092	3	41	theme	X-ray	698:702	arg1	diffraction					704:714	X-ray diffraction	698:714	X-ray diffraction	698:714	Physical-chemical characterizations of particles and hydrogels included scanning electron microscopy, energy-dispersive spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetry, porosity, compressive strength/elastic modulus, swelling degree, and cell viability.
32985092	3	42	theme	hydrogels	576:584	arg1	characterizations					541:557	Physical-chemical characterizations	523:557	Physical-chemical characterizations of particles and hydrogels	523:584	Physical-chemical characterizations of particles and hydrogels included scanning electron microscopy, energy-dispersive spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetry, porosity, compressive strength/elastic modulus, swelling degree, and cell viability.
32985092	2	43	theme	composite	476:484	arg1	hydrogels					486:494	the composite hydrogels	472:494	the composite hydrogels in fractions up to 15 wt%	472:520	HA and HASr were synthesized by a wet-precipitation method and added to the composite hydrogels in fractions up to 15 wt%.
32985092	3	44	theme	cell	804:807	arg1	viability					809:817	cell viability	804:817	cell viability	804:817	Physical-chemical characterizations of particles and hydrogels included scanning electron microscopy, energy-dispersive spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetry, porosity, compressive strength/elastic modulus, swelling degree, and cell viability.
32985092	8	45	theme	HA	1159:1160	arg1	fraction					1147:1154	the fraction	1143:1154	the fraction of HA or HASr	1143:1168	The mechanical strength was improved as the fraction of HA or HASr was increased.
32985092	2	46	from	hydrogels	486:494	arg1	fractions					499:507	fractions	499:507	fractions up to 15 wt%	499:520	HA and HASr were synthesized by a wet-precipitation method and added to the composite hydrogels in fractions up to 15 wt%.
32985092	4	47	theme	narrow	875:880	arg1	variation					887:895	narrow size variation	875:895	narrow size variation	875:895	Particles were irregular in shape and appeared to have narrow size variation.
32985092	5	48	theme	thermal	902:908	arg1	behavior					910:917	The thermal behavior	898:917	The thermal behavior of composite hydrogels	898:940	The thermal behavior of composite hydrogels was altered compared to the control (bare) hydrogel.
32985092	0	49	theme	poly	34:37	arg1	alcohol					45:51	poly(vinyl alcohol)	34:52	poly(vinyl alcohol)	34:52	Synthesis and characterization of poly(vinyl alcohol)/chondroitin sulfate composite hydrogels containing strontium-doped hydroxyapatite as promising biomaterials.
32985092	10	50	located	observed	1358:1365	arg2	%					1351:1351	above 120%	1342:1351	above 120%	1342:1351	In cell viability essay, increased cell growth (above 120%) was observed in all groups including the control hydrogel, suggesting a bioactive effect.
32985092	10	50	located	observed	1358:1365	arg1	groups					1374:1379	all groups	1370:1379	all groups including the control hydrogel	1370:1410	In cell viability essay, increased cell growth (above 120%) was observed in all groups including the control hydrogel, suggesting a bioactive effect.
32985092	10	50	located	observed	1358:1365	arg2	growth					1334:1339	increased cell growth	1319:1339	increased cell growth (above 120%)	1319:1352	In cell viability essay, increased cell growth (above 120%) was observed in all groups including the control hydrogel, suggesting a bioactive effect.
32985092	10	50	located	observed	1358:1365	arg1	hydrogel					1403:1410	the control hydrogel	1391:1410	the control hydrogel	1391:1410	In cell viability essay, increased cell growth (above 120%) was observed in all groups including the control hydrogel, suggesting a bioactive effect.
32985092	10	50	located	observed	1358:1365	arg1	essay					1312:1316	cell viability essay	1297:1316	cell viability essay	1297:1316	In cell viability essay, increased cell growth (above 120%) was observed in all groups including the control hydrogel, suggesting a bioactive effect.
32985092	2	51	dep	%	520:520	arg1	up					509:510	up	509:510	up	509:510	HA and HASr were synthesized by a wet-precipitation method and added to the composite hydrogels in fractions up to 15 wt%.
32985092	11	52	theme	PVA/CS	1459:1464	arg1	hydrogels					1466:1474	PVA/CS hydrogels	1459:1474	PVA/CS hydrogels containing HA or HASr particles	1459:1506	In conclusion, PVA/CS hydrogels containing HA or HASr particles were successfully synthesized and showed promising morphological, mechanical, and swelling properties, which are particularly required for scaffolding.
32985092	3	53	theme	energy-dispersive	625:641	arg1	spectroscopy					643:654	energy-dispersive spectroscopy	625:654	energy-dispersive spectroscopy	625:654	Physical-chemical characterizations of particles and hydrogels included scanning electron microscopy, energy-dispersive spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetry, porosity, compressive strength/elastic modulus, swelling degree, and cell viability.
32985092	1	54	theme	composite	218:226	arg1	hydrogels					228:236	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels	163:236	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles	163:299	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
32985092	0	55	theme	alcohol	45:51	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of poly(vinyl alcohol)/chondroitin sulfate composite hydrogels containing strontium-doped hydroxyapatite as promising biomaterials.
32985092	0	55	theme	alcohol	45:51	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of poly(vinyl alcohol)/chondroitin sulfate composite hydrogels containing strontium-doped hydroxyapatite as promising biomaterials.
32985092	8	56	theme	HASr	1165:1168	arg1	fraction					1147:1154	the fraction	1143:1154	the fraction of HA or HASr	1143:1168	The mechanical strength was improved as the fraction of HA or HASr was increased.
32985092	1	57	contain	containing	238:247	arg1	hydrogels					228:236	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels	163:236	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles	163:299	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
32985092	1	57	contain	containing	238:247	arg2	HA					265:266	HA	265:266	HA	265:266	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
32985092	1	57	contain	containing	238:247	arg2	particles					291:299	Sr-doped HA (HASr) particles	272:299	Sr-doped HA (HASr) particles	272:299	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
32985092	1	57	contain	containing	238:247	arg2	hydroxyapatite					249:262	hydroxyapatite	249:262	hydroxyapatite (HA)	249:267	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
32985092	7	58	theme	pore	1092:1095	arg1	size					1097:1100	pore size	1092:1100	pore size	1092:1100	HA/HASr particles reduced total porosity without reducing pore size.
32985092	5	59	theme	control	970:976	arg1	hydrogel					985:992	the control (bare) hydrogel	966:992	the control (bare) hydrogel	966:992	The thermal behavior of composite hydrogels was altered compared to the control (bare) hydrogel.
32985092	0	60	theme	vinyl	39:43	arg1	alcohol					45:51	poly(vinyl alcohol)	34:52	poly(vinyl alcohol)	34:52	Synthesis and characterization of poly(vinyl alcohol)/chondroitin sulfate composite hydrogels containing strontium-doped hydroxyapatite as promising biomaterials.
32985092	11	61	contain	containing	1476:1485	arg2	HASr					1493:1496	HASr	1493:1496	HASr	1493:1496	In conclusion, PVA/CS hydrogels containing HA or HASr particles were successfully synthesized and showed promising morphological, mechanical, and swelling properties, which are particularly required for scaffolding.
32985092	11	61	contain	containing	1476:1485	arg2	HA					1487:1488	HA	1487:1488	HA	1487:1488	In conclusion, PVA/CS hydrogels containing HA or HASr particles were successfully synthesized and showed promising morphological, mechanical, and swelling properties, which are particularly required for scaffolding.
32985092	11	61	contain	containing	1476:1485	arg1	hydrogels					1466:1474	PVA/CS hydrogels	1459:1474	PVA/CS hydrogels containing HA or HASr particles	1459:1506	In conclusion, PVA/CS hydrogels containing HA or HASr particles were successfully synthesized and showed promising morphological, mechanical, and swelling properties, which are particularly required for scaffolding.
32985092	4	62	theme	size	882:885	arg1	variation					887:895	narrow size variation	875:895	narrow size variation	875:895	Particles were irregular in shape and appeared to have narrow size variation.
32985092	11	63	theme	swelling	1590:1597	arg1	properties					1599:1608	promising morphological, mechanical, and swelling properties	1549:1608	promising morphological, mechanical, and swelling properties	1549:1608	In conclusion, PVA/CS hydrogels containing HA or HASr particles were successfully synthesized and showed promising morphological, mechanical, and swelling properties, which are particularly required for scaffolding.
32985092	3	64	theme	Fourier-transform	657:673	arg1	spectroscopy					684:695	Fourier-transform infrared spectroscopy	657:695	Fourier-transform infrared spectroscopy	657:695	Physical-chemical characterizations of particles and hydrogels included scanning electron microscopy, energy-dispersive spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetry, porosity, compressive strength/elastic modulus, swelling degree, and cell viability.
32985092	0	65	contain	containing	94:103	arg2	hydroxyapatite					121:134	strontium-doped hydroxyapatite	105:134	strontium-doped hydroxyapatite	105:134	Synthesis and characterization of poly(vinyl alcohol)/chondroitin sulfate composite hydrogels containing strontium-doped hydroxyapatite as promising biomaterials.
32985092	0	65	contain	containing	94:103	arg1	hydrogels					84:92	sulfate composite hydrogels	66:92	sulfate composite hydrogels containing strontium-doped hydroxyapatite as promising biomaterials	66:160	Synthesis and characterization of poly(vinyl alcohol)/chondroitin sulfate composite hydrogels containing strontium-doped hydroxyapatite as promising biomaterials.
32985092	0	65	contain	containing	94:103	arg2	biomaterials					149:160	promising biomaterials	139:160	promising biomaterials	139:160	Synthesis and characterization of poly(vinyl alcohol)/chondroitin sulfate composite hydrogels containing strontium-doped hydroxyapatite as promising biomaterials.
32985092	5	66	theme	bare	979:982	arg1	hydrogel					985:992	the control (bare) hydrogel	966:992	the control (bare) hydrogel	966:992	The thermal behavior of composite hydrogels was altered compared to the control (bare) hydrogel.
32985092	9	67	theme	faster	1209:1214	arg1	uptake					1222:1227	a faster water uptake	1207:1227	a faster water uptake	1207:1227	HASr particles led to a faster water uptake but did not interfere with the total hydrogel swelling capacity.
32985092	3	68	theme	infrared	675:682	arg1	spectroscopy					684:695	Fourier-transform infrared spectroscopy	657:695	Fourier-transform infrared spectroscopy	657:695	Physical-chemical characterizations of particles and hydrogels included scanning electron microscopy, energy-dispersive spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetry, porosity, compressive strength/elastic modulus, swelling degree, and cell viability.
32985092	11	69	theme	morphological	1559:1571	arg1	properties					1599:1608	promising morphological, mechanical, and swelling properties	1549:1608	promising morphological, mechanical, and swelling properties	1549:1608	In conclusion, PVA/CS hydrogels containing HA or HASr particles were successfully synthesized and showed promising morphological, mechanical, and swelling properties, which are particularly required for scaffolding.
32985092	7	70	theme	total	1060:1064	arg1	porosity					1066:1073	total porosity	1060:1073	total porosity	1060:1073	HA/HASr particles reduced total porosity without reducing pore size.
32985092	1	71	theme	Novel	163:167	arg1	PVA/CS					210:215	PVA/CS	210:215	PVA/CS	210:215	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
32985092	1	71	theme	Novel	163:167	arg1	sulfate					201:207	Novel poly(vinyl alcohol)/chondroitin sulfate	163:207	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles	163:299	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
32985092	3	72	theme	strength/elastic	757:772	arg1	modulus					774:780	compressive strength/elastic modulus	745:780	compressive strength/elastic modulus	745:780	Physical-chemical characterizations of particles and hydrogels included scanning electron microscopy, energy-dispersive spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetry, porosity, compressive strength/elastic modulus, swelling degree, and cell viability.
32985092	7	73	theme	HA/HASr	1034:1040	arg1	particles					1042:1050	HA/HASr particles	1034:1050	HA/HASr particles	1034:1050	HA/HASr particles reduced total porosity without reducing pore size.
32985092	1	74	theme	poly	169:172	arg1	PVA/CS					210:215	PVA/CS	210:215	PVA/CS	210:215	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
32985092	1	74	theme	poly	169:172	arg1	sulfate					201:207	Novel poly(vinyl alcohol)/chondroitin sulfate	163:207	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles	163:299	Novel poly(vinyl alcohol)/chondroitin sulfate (PVA/CS) composite hydrogels containing hydroxyapatite (HA) or Sr-doped HA (HASr) particles were synthesized by a freeze/thaw method and characterized aiming towards biomedical applications.
33173002	9	0	theme	sugar	1653:1657	arg1	metabolism					1659:1668	amino sugar and nucleotide sugar metabolism	1626:1668	metabolism	1659:1668	Moreover, SomA was found to globally regulate glucose uptake and utilization, as well as amino sugar and nucleotide sugar metabolism, which provides precursors for polysaccharide synthesis.
33173002	2	1	theme	assembly	232:239	arg1	pathways					256:263	distinct assembly and regulation pathways	223:263	distinct assembly and regulation pathways	223:263	Despite having distinct assembly and regulation pathways, matrix exopolysaccharide and cell wall polysaccharides share common substrates and intermediates in their biosynthetic pathways.
33173002	6	2	theme	cell	1203:1206	arg1	sensors					1220:1226	the cell wall stress sensors	1199:1226	the cell wall stress sensors	1199:1226	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	12	3	theme	cell	2548:2551	arg1	homeostasis					2558:2568	cell wall homeostasis	2548:2568	cell wall homeostasis	2548:2568	Here, we demonstrate that caspofungin and other cell wall stressors can induce galactosaminogalactan (GAG)-dependent biofilm formation in the human pathogen Aspergillus fumigatus We further identified SomA as a master transcription factor playing a dual role in both biofilm formation and cell wall homeostasis.
33173002	2	4	theme	wall	300:303	arg1	polysaccharides					305:319	cell wall polysaccharides	295:319	cell wall polysaccharides	295:319	Despite having distinct assembly and regulation pathways, matrix exopolysaccharide and cell wall polysaccharides share common substrates and intermediates in their biosynthetic pathways.
33173002	13	5	theme	wall	2698:2701	arg1	sensors					2710:2716	cell wall stress sensors	2693:2716	cell wall stress sensors	2693:2716	SomA plays this dual role by direct binding to a conserved motif upstream of GAG biosynthetic genes and genes involved in cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	14	6	theme	cell	2872:2875	arg1	homeostasis					2882:2892	cell wall homeostasis	2872:2892	cell wall homeostasis	2872:2892	Collectively, these findings reveal a transcriptional control pathway that integrates biofilm formation and cell wall homeostasis and suggest SomA as an attractive target for antifungal drug development.
33173002	4	7	theme	humans	702:707	arg1	pathogen					690:697	the major fungal pathogen	673:697	the major fungal pathogen of humans Aspergillus fumigatus	673:729	Here, we demonstrate that cell wall stress promotes production of the exopolysaccharide galactosaminogalactan (GAG)-depend biofilm formation in the major fungal pathogen of humans Aspergillus fumigatus and that the transcription factor SomA plays a crucial role in mediating this process.
33173002	1	8	theme	wall	183:186	arg1	Polysaccharides					124:138	Polysaccharides	124:138	Polysaccharides	124:138	Polysaccharides are key components of both the fungal cell wall and biofilm matrix.
33173002	1	8	theme	wall	183:186	arg1	components					148:157	key components	144:157	key components of both the fungal cell wall and biofilm matrix	144:205	Polysaccharides are key components of both the fungal cell wall and biofilm matrix.
33173002	11	9	theme	echinocandin	2137:2148	arg1	drugs					2150:2154	clinically approved echinocandin drugs	2117:2154	clinically approved echinocandin drugs	2117:2154	Caspofungin is a member of a new class of clinically approved echinocandin drugs to treat invasive aspergillosis by blocking β-1,3-glucan synthase, thus damaging the fungal cell wall.
33173002	6	10	theme	β-1,3-glucan	1251:1262	arg1	synthase					1264:1271	β-1,3-glucan synthase	1251:1271	β-1,3-glucan synthase	1251:1271	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	1	11	theme	biofilm	192:198	arg1	matrix					200:205	biofilm matrix	192:205	biofilm matrix	192:205	Polysaccharides are key components of both the fungal cell wall and biofilm matrix.
33173002	6	12	theme	upstream	1164:1171	arg1	regions					1173:1179	the upstream regions	1160:1179	the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase	1160:1271	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	4	13	theme	major	677:681	arg1	pathogen					690:697	the major fungal pathogen	673:697	the major fungal pathogen of humans Aspergillus fumigatus	673:729	Here, we demonstrate that cell wall stress promotes production of the exopolysaccharide galactosaminogalactan (GAG)-depend biofilm formation in the major fungal pathogen of humans Aspergillus fumigatus and that the transcription factor SomA plays a crucial role in mediating this process.
33173002	10	14	theme	adaptive	1779:1786	arg1	mechanisms					1788:1797	fungal adaptive mechanisms	1772:1797	fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention	1772:2072	Collectively, our work provides insight into fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention.
33173002	5	15	theme	transcriptome	869:881	arg1	sequencing					883:892	transcriptome sequencing	869:892	transcriptome sequencing	869:892	A core set of SomA target genes were identified by transcriptome sequencing and chromatin immunoprecipitation coupled to sequencing (ChIP-Seq).
33173002	8	16	theme	cell	1445:1448	arg1	architecture					1455:1466	normal cell wall architecture	1438:1466	normal cell wall architecture	1438:1466	Consistent with these findings, SomA is required for the maintenance of normal cell wall architecture and compositions in addition to its function in biofilm development.
33173002	10	17	theme	wall	1819:1822	arg1	stress					1824:1829	cell wall stress	1814:1829	cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention	1814:2072	Collectively, our work provides insight into fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention.
33173002	11	18	dep	member	2092:2097	arg1	damaging					2228:2235	damaging	2228:2235	damaging	2228:2235	Caspofungin is a member of a new class of clinically approved echinocandin drugs to treat invasive aspergillosis by blocking β-1,3-glucan synthase, thus damaging the fungal cell wall.
33173002	8	19	theme	architecture	1455:1466	arg1	maintenance					1423:1433	the maintenance	1419:1433	the maintenance of normal cell wall architecture and compositions in addition to its function in biofilm development	1419:1534	Consistent with these findings, SomA is required for the maintenance of normal cell wall architecture and compositions in addition to its function in biofilm development.
33173002	14	20	theme	transcriptional	2802:2816	arg1	pathway					2826:2832	a transcriptional control pathway	2800:2832	a transcriptional control pathway that integrates biofilm formation and cell wall homeostasis	2800:2892	Collectively, these findings reveal a transcriptional control pathway that integrates biofilm formation and cell wall homeostasis and suggest SomA as an attractive target for antifungal drug development.
33173002	2	21	theme	distinct	223:230	arg1	pathways					256:263	distinct assembly and regulation pathways	223:263	distinct assembly and regulation pathways	223:263	Despite having distinct assembly and regulation pathways, matrix exopolysaccharide and cell wall polysaccharides share common substrates and intermediates in their biosynthetic pathways.
33173002	11	22	theme	class	2108:2112	arg1	Caspofungin					2075:2085	Caspofungin	2075:2085	Caspofungin	2075:2085	Caspofungin is a member of a new class of clinically approved echinocandin drugs to treat invasive aspergillosis by blocking β-1,3-glucan synthase, thus damaging the fungal cell wall.
33173002	11	22	theme	class	2108:2112	arg1	member					2092:2097	a member	2090:2097	a member of a new class of clinically approved echinocandin drugs to treat invasive aspergillosis by blocking β-1,3-glucan synthase	2090:2220	Caspofungin is a member of a new class of clinically approved echinocandin drugs to treat invasive aspergillosis by blocking β-1,3-glucan synthase, thus damaging the fungal cell wall.
33173002	6	23	theme	novel	978:982	arg1	site					997:1000	a novel SomA-binding site	976:1000	a novel SomA-binding site	976:1000	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	8	24	theme	compositions	1472:1483	arg1	maintenance					1423:1433	the maintenance	1419:1433	the maintenance of normal cell wall architecture and compositions in addition to its function in biofilm development	1419:1534	Consistent with these findings, SomA is required for the maintenance of normal cell wall architecture and compositions in addition to its function in biofilm development.
33173002	8	25	with	Consistent	1366:1375	arg1	findings					1388:1395	these findings	1382:1395	these findings	1382:1395	Consistent with these findings, SomA is required for the maintenance of normal cell wall architecture and compositions in addition to its function in biofilm development.
33173002	12	26	dep	pathogen	2407:2414	arg1	identified					2449:2458	identified	2449:2458	identified SomA as a master transcription factor playing a dual role in both biofilm formation and cell wall homeostasis	2449:2568	Here, we demonstrate that caspofungin and other cell wall stressors can induce galactosaminogalactan (GAG)-dependent biofilm formation in the human pathogen Aspergillus fumigatus We further identified SomA as a master transcription factor playing a dual role in both biofilm formation and cell wall homeostasis.
33173002	2	27	theme	matrix	266:271	arg1	exopolysaccharide					273:289	matrix exopolysaccharide	266:289	matrix exopolysaccharide	266:289	Despite having distinct assembly and regulation pathways, matrix exopolysaccharide and cell wall polysaccharides share common substrates and intermediates in their biosynthetic pathways.
33173002	11	28	theme	fungal	2241:2246	arg1	wall					2253:2256	the fungal cell wall	2237:2256	the fungal cell wall	2237:2256	Caspofungin is a member of a new class of clinically approved echinocandin drugs to treat invasive aspergillosis by blocking β-1,3-glucan synthase, thus damaging the fungal cell wall.
33173002	7	29	theme	polysaccharide	1337:1350	arg1	biosynthesis					1352:1363	cell wall polysaccharide biosynthesis	1327:1363	cell wall polysaccharide biosynthesis	1327:1363	Thus, SomA plays a direct regulation of both GAG and cell wall polysaccharide biosynthesis.
33173002	10	30	theme	cell	2024:2027	arg1	biosynthesis					2034:2045	cell wall biosynthesis	2024:2045	cell wall biosynthesis	2024:2045	Collectively, our work provides insight into fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention.
33173002	13	31	theme	biosynthetic	2652:2663	arg1	genes					2665:2669	GAG biosynthetic genes	2648:2669	GAG biosynthetic genes	2648:2669	SomA plays this dual role by direct binding to a conserved motif upstream of GAG biosynthetic genes and genes involved in cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	10	32	from	mechanisms	1788:1797	arg1	response					1802:1809	response	1802:1809	response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention	1802:2072	Collectively, our work provides insight into fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention.
33173002	4	33	theme	transcription	744:756	arg1	SomA					765:768	the transcription factor SomA	740:768	the transcription factor SomA	740:768	Here, we demonstrate that cell wall stress promotes production of the exopolysaccharide galactosaminogalactan (GAG)-depend biofilm formation in the major fungal pathogen of humans Aspergillus fumigatus and that the transcription factor SomA plays a crucial role in mediating this process.
33173002	0	34	theme	Wall	81:84	arg1	Homeostasis					86:96	Cell Wall Homeostasis	76:96	Cell Wall Homeostasis	76:96	The Transcription Factor SomA Synchronously Regulates Biofilm Formation and Cell Wall Homeostasis in Aspergillus fumigatus.
33173002	1	35	theme	fungal	171:176	arg1	wall					183:186	the fungal cell wall	167:186	the fungal cell wall	167:186	Polysaccharides are key components of both the fungal cell wall and biofilm matrix.
33173002	6	36	theme	genes	1046:1050	arg1	regions					1018:1024	the promoter regions	1005:1024	the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA	1005:1105	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	6	37	dep	genes	1046:1050	arg1	genes					1046:1050	GAG biosynthetic genes agd3 and ega3	1029:1064	GAG biosynthetic genes agd3 and ega3	1029:1064	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	6	37	dep	genes	1046:1050	arg1	ega3					1061:1064	ega3	1061:1064	ega3	1061:1064	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	6	37	dep	genes	1046:1050	arg1	agd3					1052:1055	agd3	1052:1055	agd3	1052:1055	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	6	37	dep	genes	1046:1050	arg1	stuA					1102:1105	stuA	1102:1105	stuA	1102:1105	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	6	37	dep	genes	1046:1050	arg1	medA					1093:1096	medA	1093:1096	medA	1093:1096	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	6	38	theme	GAG	1029:1031	arg1	genes					1046:1050	GAG biosynthetic genes agd3 and ega3	1029:1064	GAG biosynthetic genes agd3 and ega3	1029:1064	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	6	38	theme	GAG	1029:1031	arg1	ega3					1061:1064	ega3	1061:1064	ega3	1061:1064	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	6	38	theme	GAG	1029:1031	arg1	agd3					1052:1055	agd3	1052:1055	agd3	1052:1055	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	6	38	theme	GAG	1029:1031	arg1	stuA					1102:1105	stuA	1102:1105	stuA	1102:1105	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	6	38	theme	GAG	1029:1031	arg1	medA					1093:1096	medA	1093:1096	medA	1093:1096	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	7	39	theme	wall	1332:1335	arg1	biosynthesis					1352:1363	cell wall polysaccharide biosynthesis	1327:1363	cell wall polysaccharide biosynthesis	1327:1363	Thus, SomA plays a direct regulation of both GAG and cell wall polysaccharide biosynthesis.
33173002	13	40	theme	GAG	2648:2650	arg1	genes					2665:2669	GAG biosynthetic genes	2648:2669	GAG biosynthetic genes	2648:2669	SomA plays this dual role by direct binding to a conserved motif upstream of GAG biosynthetic genes and genes involved in cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	13	41	theme	direct	2600:2605	arg1	binding					2607:2613	direct binding	2600:2613	direct binding to a conserved motif upstream of GAG biosynthetic genes and genes involved in cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase	2600:2761	SomA plays this dual role by direct binding to a conserved motif upstream of GAG biosynthetic genes and genes involved in cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	5	42	theme	genes	844:848	arg1	genes					844:848	SomA target genes	832:848	SomA target genes	832:848	A core set of SomA target genes were identified by transcriptome sequencing and chromatin immunoprecipitation coupled to sequencing (ChIP-Seq).
33173002	5	42	theme	genes	844:848	arg1	set					825:827	A core set	818:827	A core set of SomA target genes	818:848	A core set of SomA target genes were identified by transcriptome sequencing and chromatin immunoprecipitation coupled to sequencing (ChIP-Seq).
33173002	11	43	theme	invasive	2165:2172	arg1	aspergillosis					2174:2186	invasive aspergillosis	2165:2186	invasive aspergillosis	2165:2186	Caspofungin is a member of a new class of clinically approved echinocandin drugs to treat invasive aspergillosis by blocking β-1,3-glucan synthase, thus damaging the fungal cell wall.
33173002	6	44	theme	stress	1213:1218	arg1	sensors					1220:1226	the cell wall stress sensors	1199:1226	the cell wall stress sensors	1199:1226	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	3	45	theme	polysaccharides	484:498	arg1	production					464:473	the production	460:473	the production of these polysaccharides	460:498	It is not clear, however, if the biosynthetic pathways governing the production of these polysaccharides are cooperatively regulated.
33173002	4	46	from	production	581:590	arg1	pathogen					690:697	the major fungal pathogen	673:697	the major fungal pathogen of humans Aspergillus fumigatus	673:729	Here, we demonstrate that cell wall stress promotes production of the exopolysaccharide galactosaminogalactan (GAG)-depend biofilm formation in the major fungal pathogen of humans Aspergillus fumigatus and that the transcription factor SomA plays a crucial role in mediating this process.
33173002	12	47	theme	master	2470:2475	arg1	factor					2491:2496	a master transcription factor	2468:2496	a master transcription factor playing a dual role in both biofilm formation and cell wall homeostasis	2468:2568	Here, we demonstrate that caspofungin and other cell wall stressors can induce galactosaminogalactan (GAG)-dependent biofilm formation in the human pathogen Aspergillus fumigatus We further identified SomA as a master transcription factor playing a dual role in both biofilm formation and cell wall homeostasis.
33173002	12	47	theme	master	2470:2475	arg1	SomA					2460:2463	SomA	2460:2463	SomA	2460:2463	Here, we demonstrate that caspofungin and other cell wall stressors can induce galactosaminogalactan (GAG)-dependent biofilm formation in the human pathogen Aspergillus fumigatus We further identified SomA as a master transcription factor playing a dual role in both biofilm formation and cell wall homeostasis.
33173002	1	48	theme	cell	178:181	arg1	wall					183:186	the fungal cell wall	167:186	the fungal cell wall	167:186	Polysaccharides are key components of both the fungal cell wall and biofilm matrix.
33173002	13	49	theme	dual	2587:2590	arg1	role					2592:2595	this dual role	2582:2595	this dual role	2582:2595	SomA plays this dual role by direct binding to a conserved motif upstream of GAG biosynthetic genes and genes involved in cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	11	50	theme	β-1,3-glucan	2200:2211	arg1	synthase					2213:2220	β-1,3-glucan synthase	2200:2220	β-1,3-glucan synthase	2200:2220	Caspofungin is a member of a new class of clinically approved echinocandin drugs to treat invasive aspergillosis by blocking β-1,3-glucan synthase, thus damaging the fungal cell wall.
33173002	4	51	theme	cell	555:558	arg1	stress					565:570	cell wall stress	555:570	cell wall stress	555:570	Here, we demonstrate that cell wall stress promotes production of the exopolysaccharide galactosaminogalactan (GAG)-depend biofilm formation in the major fungal pathogen of humans Aspergillus fumigatus and that the transcription factor SomA plays a crucial role in mediating this process.
33173002	10	52	theme	cell	1859:1862	arg1	homeostasis					1869:1879	cell wall homeostasis	1859:1879	cell wall homeostasis	1859:1879	Collectively, our work provides insight into fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention.
33173002	2	53	theme	biosynthetic	372:383	arg1	pathways					385:392	their biosynthetic pathways	366:392	their biosynthetic pathways	366:392	Despite having distinct assembly and regulation pathways, matrix exopolysaccharide and cell wall polysaccharides share common substrates and intermediates in their biosynthetic pathways.
33173002	5	54	theme	SomA	832:835	arg1	genes					844:848	SomA target genes	832:848	SomA target genes	832:848	A core set of SomA target genes were identified by transcriptome sequencing and chromatin immunoprecipitation coupled to sequencing (ChIP-Seq).
33173002	7	55	theme	biosynthesis	1352:1363	arg1	regulation					1300:1309	a direct regulation	1291:1309	a direct regulation of both GAG and cell wall polysaccharide biosynthesis	1291:1363	Thus, SomA plays a direct regulation of both GAG and cell wall polysaccharide biosynthesis.
33173002	0	56	dep	Factor	18:23	arg1	SomA					25:28	SomA	25:28	The Transcription Factor SomA	0:28	The Transcription Factor SomA Synchronously Regulates Biofilm Formation and Cell Wall Homeostasis in Aspergillus fumigatus.
33173002	4	57	theme	-depend	644:650	arg1	formation					660:668	the exopolysaccharide galactosaminogalactan (GAG)-depend biofilm formation	595:668	the exopolysaccharide galactosaminogalactan (GAG)-depend biofilm formation	595:668	Here, we demonstrate that cell wall stress promotes production of the exopolysaccharide galactosaminogalactan (GAG)-depend biofilm formation in the major fungal pathogen of humans Aspergillus fumigatus and that the transcription factor SomA plays a crucial role in mediating this process.
33173002	8	58	theme	normal	1438:1443	arg1	architecture					1455:1466	normal cell wall architecture	1438:1466	normal cell wall architecture	1438:1466	Consistent with these findings, SomA is required for the maintenance of normal cell wall architecture and compositions in addition to its function in biofilm development.
33173002	6	59	theme	SomA-binding	1124:1135	arg1	site					1137:1140	this SomA-binding site	1119:1140	this SomA-binding site	1119:1140	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	4	60	theme	formation	660:668	arg1	production					581:590	production	581:590	production of the exopolysaccharide galactosaminogalactan (GAG)-depend biofilm formation in the major fungal pathogen of humans Aspergillus fumigatus	581:729	Here, we demonstrate that cell wall stress promotes production of the exopolysaccharide galactosaminogalactan (GAG)-depend biofilm formation in the major fungal pathogen of humans Aspergillus fumigatus and that the transcription factor SomA plays a crucial role in mediating this process.
33173002	9	61	theme	nucleotide	1642:1651	arg1	metabolism					1659:1668	amino sugar and nucleotide sugar metabolism	1626:1668	metabolism	1659:1668	Moreover, SomA was found to globally regulate glucose uptake and utilization, as well as amino sugar and nucleotide sugar metabolism, which provides precursors for polysaccharide synthesis.
33173002	12	62	theme	wall	2553:2556	arg1	homeostasis					2558:2568	cell wall homeostasis	2548:2568	cell wall homeostasis	2548:2568	Here, we demonstrate that caspofungin and other cell wall stressors can induce galactosaminogalactan (GAG)-dependent biofilm formation in the human pathogen Aspergillus fumigatus We further identified SomA as a master transcription factor playing a dual role in both biofilm formation and cell wall homeostasis.
33173002	12	63	theme	biofilm	2376:2382	arg1	formation					2384:2392	galactosaminogalactan (GAG)-dependent biofilm formation	2338:2392	galactosaminogalactan (GAG)-dependent biofilm formation	2338:2392	Here, we demonstrate that caspofungin and other cell wall stressors can induce galactosaminogalactan (GAG)-dependent biofilm formation in the human pathogen Aspergillus fumigatus We further identified SomA as a master transcription factor playing a dual role in both biofilm formation and cell wall homeostasis.
33173002	11	64	theme	approved	2128:2135	arg1	drugs					2150:2154	clinically approved echinocandin drugs	2117:2154	clinically approved echinocandin drugs	2117:2154	Caspofungin is a member of a new class of clinically approved echinocandin drugs to treat invasive aspergillosis by blocking β-1,3-glucan synthase, thus damaging the fungal cell wall.
33173002	10	65	theme	fungal	1772:1777	arg1	mechanisms					1788:1797	fungal adaptive mechanisms	1772:1797	fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention	1772:2072	Collectively, our work provides insight into fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention.
33173002	8	66	dep	function	1504:1511	arg1	addition					1488:1495	addition	1488:1495	addition	1488:1495	Consistent with these findings, SomA is required for the maintenance of normal cell wall architecture and compositions in addition to its function in biofilm development.
33173002	14	67	theme	wall	2877:2880	arg1	homeostasis					2882:2892	cell wall homeostasis	2872:2892	cell wall homeostasis	2872:2892	Collectively, these findings reveal a transcriptional control pathway that integrates biofilm formation and cell wall homeostasis and suggest SomA as an attractive target for antifungal drug development.
33173002	2	68	theme	cell	295:298	arg1	polysaccharides					305:319	cell wall polysaccharides	295:319	cell wall polysaccharides	295:319	Despite having distinct assembly and regulation pathways, matrix exopolysaccharide and cell wall polysaccharides share common substrates and intermediates in their biosynthetic pathways.
33173002	13	69	theme	stress	2703:2708	arg1	sensors					2710:2716	cell wall stress sensors	2693:2716	cell wall stress sensors	2693:2716	SomA plays this dual role by direct binding to a conserved motif upstream of GAG biosynthetic genes and genes involved in cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	5	70	theme	chromatin	898:906	arg1	immunoprecipitation					908:926	chromatin immunoprecipitation	898:926	chromatin immunoprecipitation	898:926	A core set of SomA target genes were identified by transcriptome sequencing and chromatin immunoprecipitation coupled to sequencing (ChIP-Seq).
33173002	0	71	theme	Biofilm	54:60	arg1	Formation					62:70	Biofilm Formation	54:70	Biofilm Formation	54:70	The Transcription Factor SomA Synchronously Regulates Biofilm Formation and Cell Wall Homeostasis in Aspergillus fumigatus.
33173002	6	72	theme	genes	1184:1188	arg1	regions					1173:1179	the upstream regions	1160:1179	the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase	1160:1271	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	12	73	theme	biofilm	2526:2532	arg1	formation					2534:2542	biofilm formation	2526:2542	biofilm formation	2526:2542	Here, we demonstrate that caspofungin and other cell wall stressors can induce galactosaminogalactan (GAG)-dependent biofilm formation in the human pathogen Aspergillus fumigatus We further identified SomA as a master transcription factor playing a dual role in both biofilm formation and cell wall homeostasis.
33173002	10	74	theme	fungal	1952:1957	arg1	viability					1959:1967	fungal viability	1952:1967	fungal viability	1952:1967	Collectively, our work provides insight into fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention.
33173002	14	75	theme	drug	2950:2953	arg1	development					2955:2965	antifungal drug development	2939:2965	antifungal drug development	2939:2965	Collectively, these findings reveal a transcriptional control pathway that integrates biofilm formation and cell wall homeostasis and suggest SomA as an attractive target for antifungal drug development.
33173002	12	76	theme	wall	2312:2315	arg1	stressors					2317:2325	other cell wall stressors	2301:2325	other cell wall stressors	2301:2325	Here, we demonstrate that caspofungin and other cell wall stressors can induce galactosaminogalactan (GAG)-dependent biofilm formation in the human pathogen Aspergillus fumigatus We further identified SomA as a master transcription factor playing a dual role in both biofilm formation and cell wall homeostasis.
33173002	11	77	theme	drugs	2150:2154	arg1	class					2108:2112	a new class	2102:2112	a new class of clinically approved echinocandin drugs to treat invasive aspergillosis by blocking β-1,3-glucan synthase	2102:2220	Caspofungin is a member of a new class of clinically approved echinocandin drugs to treat invasive aspergillosis by blocking β-1,3-glucan synthase, thus damaging the fungal cell wall.
33173002	14	78	theme	biofilm	2850:2856	arg1	formation					2858:2866	biofilm formation	2850:2866	biofilm formation	2850:2866	Collectively, these findings reveal a transcriptional control pathway that integrates biofilm formation and cell wall homeostasis and suggest SomA as an attractive target for antifungal drug development.
33173002	4	79	theme	Aspergillus	709:719	arg1	humans					702:707	humans	702:707	humans Aspergillus fumigatus	702:729	Here, we demonstrate that cell wall stress promotes production of the exopolysaccharide galactosaminogalactan (GAG)-depend biofilm formation in the major fungal pathogen of humans Aspergillus fumigatus and that the transcription factor SomA plays a crucial role in mediating this process.
33173002	10	80	theme	human	1988:1992	arg1	hosts					1994:1998	human hosts	1988:1998	human hosts	1988:1998	Collectively, our work provides insight into fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention.
33173002	2	81	theme	common	327:332	arg1	substrates					334:343	common substrates	327:343	common substrates	327:343	Despite having distinct assembly and regulation pathways, matrix exopolysaccharide and cell wall polysaccharides share common substrates and intermediates in their biosynthetic pathways.
33173002	1	82	theme	matrix	200:205	arg1	Polysaccharides					124:138	Polysaccharides	124:138	Polysaccharides	124:138	Polysaccharides are key components of both the fungal cell wall and biofilm matrix.
33173002	1	82	theme	matrix	200:205	arg1	components					148:157	key components	144:157	key components of both the fungal cell wall and biofilm matrix	144:205	Polysaccharides are key components of both the fungal cell wall and biofilm matrix.
33173002	6	83	theme	promoter	1009:1016	arg1	regions					1018:1024	the promoter regions	1005:1024	the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA	1005:1105	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	9	84	theme	polysaccharide	1701:1714	arg1	synthesis					1716:1724	polysaccharide synthesis	1701:1724	polysaccharide synthesis	1701:1724	Moreover, SomA was found to globally regulate glucose uptake and utilization, as well as amino sugar and nucleotide sugar metabolism, which provides precursors for polysaccharide synthesis.
33173002	8	85	theme	wall	1450:1453	arg1	architecture					1455:1466	normal cell wall architecture	1438:1466	normal cell wall architecture	1438:1466	Consistent with these findings, SomA is required for the maintenance of normal cell wall architecture and compositions in addition to its function in biofilm development.
33173002	12	86	theme	dual	2508:2511	arg1	role					2513:2516	a dual role	2506:2516	a dual role	2506:2516	Here, we demonstrate that caspofungin and other cell wall stressors can induce galactosaminogalactan (GAG)-dependent biofilm formation in the human pathogen Aspergillus fumigatus We further identified SomA as a master transcription factor playing a dual role in both biofilm formation and cell wall homeostasis.
33173002	14	87	theme	attractive	2917:2926	arg1	SomA					2906:2909	SomA	2906:2909	SomA	2906:2909	Collectively, these findings reveal a transcriptional control pathway that integrates biofilm formation and cell wall homeostasis and suggest SomA as an attractive target for antifungal drug development.
33173002	14	87	theme	attractive	2917:2926	arg1	target					2928:2933	an attractive target	2914:2933	an attractive target for antifungal drug development	2914:2965	Collectively, these findings reveal a transcriptional control pathway that integrates biofilm formation and cell wall homeostasis and suggest SomA as an attractive target for antifungal drug development.
33173002	2	88	theme	regulation	245:254	arg1	pathways					256:263	distinct assembly and regulation pathways	223:263	distinct assembly and regulation pathways	223:263	Despite having distinct assembly and regulation pathways, matrix exopolysaccharide and cell wall polysaccharides share common substrates and intermediates in their biosynthetic pathways.
33173002	13	89	theme	cell	2693:2696	arg1	sensors					2710:2716	cell wall stress sensors	2693:2716	cell wall stress sensors	2693:2716	SomA plays this dual role by direct binding to a conserved motif upstream of GAG biosynthetic genes and genes involved in cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	12	90	theme	-dependent	2365:2374	arg1	formation					2384:2392	galactosaminogalactan (GAG)-dependent biofilm formation	2338:2392	galactosaminogalactan (GAG)-dependent biofilm formation	2338:2392	Here, we demonstrate that caspofungin and other cell wall stressors can induce galactosaminogalactan (GAG)-dependent biofilm formation in the human pathogen Aspergillus fumigatus We further identified SomA as a master transcription factor playing a dual role in both biofilm formation and cell wall homeostasis.
33173002	14	91	theme	control	2818:2824	arg1	pathway					2826:2832	a transcriptional control pathway	2800:2832	a transcriptional control pathway that integrates biofilm formation and cell wall homeostasis	2800:2892	Collectively, these findings reveal a transcriptional control pathway that integrates biofilm formation and cell wall homeostasis and suggest SomA as an attractive target for antifungal drug development.
33173002	4	92	theme	fungal	683:688	arg1	pathogen					690:697	the major fungal pathogen	673:697	the major fungal pathogen of humans Aspergillus fumigatus	673:729	Here, we demonstrate that cell wall stress promotes production of the exopolysaccharide galactosaminogalactan (GAG)-depend biofilm formation in the major fungal pathogen of humans Aspergillus fumigatus and that the transcription factor SomA plays a crucial role in mediating this process.
33173002	10	93	theme	cell	1814:1817	arg1	stress					1824:1829	cell wall stress	1814:1829	cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention	1814:2072	Collectively, our work provides insight into fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention.
33173002	12	94	theme	cell	2307:2310	arg1	stressors					2317:2325	other cell wall stressors	2301:2325	other cell wall stressors	2301:2325	Here, we demonstrate that caspofungin and other cell wall stressors can induce galactosaminogalactan (GAG)-dependent biofilm formation in the human pathogen Aspergillus fumigatus We further identified SomA as a master transcription factor playing a dual role in both biofilm formation and cell wall homeostasis.
33173002	6	95	theme	SomA-binding	984:995	arg1	site					997:1000	a novel SomA-binding site	976:1000	a novel SomA-binding site	976:1000	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	11	96	theme	new	2104:2106	arg1	class					2108:2112	a new class	2102:2112	a new class of clinically approved echinocandin drugs to treat invasive aspergillosis by blocking β-1,3-glucan synthase	2102:2220	Caspofungin is a member of a new class of clinically approved echinocandin drugs to treat invasive aspergillosis by blocking β-1,3-glucan synthase, thus damaging the fungal cell wall.
33173002	6	97	located	found	1151:1155	arg1	regions					1173:1179	the upstream regions	1160:1179	the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase	1160:1271	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	6	97	located	found	1151:1155	arg2	site					1137:1140	this SomA-binding site	1119:1140	this SomA-binding site	1119:1140	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	6	98	theme	chitin	1229:1234	arg1	synthases					1236:1244	chitin synthases	1229:1244	chitin synthases	1229:1244	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	4	99	theme	factor	758:763	arg1	SomA					765:768	the transcription factor SomA	740:768	the transcription factor SomA	740:768	Here, we demonstrate that cell wall stress promotes production of the exopolysaccharide galactosaminogalactan (GAG)-depend biofilm formation in the major fungal pathogen of humans Aspergillus fumigatus and that the transcription factor SomA plays a crucial role in mediating this process.
33173002	1	100	theme	key	144:146	arg1	Polysaccharides					124:138	Polysaccharides	124:138	Polysaccharides	124:138	Polysaccharides are key components of both the fungal cell wall and biofilm matrix.
33173002	1	100	theme	key	144:146	arg1	components					148:157	key components	144:157	key components of both the fungal cell wall and biofilm matrix	144:205	Polysaccharides are key components of both the fungal cell wall and biofilm matrix.
33173002	10	101	theme	wall	2029:2032	arg1	biosynthesis					2034:2045	cell wall biosynthesis	2024:2045	cell wall biosynthesis	2024:2045	Collectively, our work provides insight into fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention.
33173002	0	102	theme	Cell	76:79	arg1	Homeostasis					86:96	Cell Wall Homeostasis	76:96	Cell Wall Homeostasis	76:96	The Transcription Factor SomA Synchronously Regulates Biofilm Formation and Cell Wall Homeostasis in Aspergillus fumigatus.
33173002	10	103	theme	biofilm	1837:1843	arg1	formation					1845:1853	biofilm formation	1837:1853	biofilm formation	1837:1853	Collectively, our work provides insight into fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention.
33173002	11	104	theme	cell	2248:2251	arg1	wall					2253:2256	the fungal cell wall	2237:2256	the fungal cell wall	2237:2256	Caspofungin is a member of a new class of clinically approved echinocandin drugs to treat invasive aspergillosis by blocking β-1,3-glucan synthase, thus damaging the fungal cell wall.
33173002	12	105	theme	other	2301:2305	arg1	stressors					2317:2325	other cell wall stressors	2301:2325	other cell wall stressors	2301:2325	Here, we demonstrate that caspofungin and other cell wall stressors can induce galactosaminogalactan (GAG)-dependent biofilm formation in the human pathogen Aspergillus fumigatus We further identified SomA as a master transcription factor playing a dual role in both biofilm formation and cell wall homeostasis.
33173002	6	106	theme	wall	1208:1211	arg1	sensors					1220:1226	the cell wall stress sensors	1199:1226	the cell wall stress sensors	1199:1226	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	8	107	theme	biofilm	1516:1522	arg1	development					1524:1534	biofilm development	1516:1534	biofilm development	1516:1534	Consistent with these findings, SomA is required for the maintenance of normal cell wall architecture and compositions in addition to its function in biofilm development.
33173002	10	108	theme	more	2059:2062	arg1	attention					2064:2072	more attention	2059:2072	more attention	2059:2072	Collectively, our work provides insight into fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention.
33173002	13	109	theme	conserved	2620:2628	arg1	motif					2630:2634	a conserved motif	2618:2634	a conserved motif upstream of GAG biosynthetic genes and genes involved in cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase	2618:2761	SomA plays this dual role by direct binding to a conserved motif upstream of GAG biosynthetic genes and genes involved in cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	4	110	dep	Aspergillus	709:719	arg1	fumigatus					721:729	fumigatus	721:729	fumigatus	721:729	Here, we demonstrate that cell wall stress promotes production of the exopolysaccharide galactosaminogalactan (GAG)-depend biofilm formation in the major fungal pathogen of humans Aspergillus fumigatus and that the transcription factor SomA plays a crucial role in mediating this process.
33173002	10	111	from	hosts	1994:1998	arg1	essential					1938:1946	essential	1938:1946	essential	1938:1946	Collectively, our work provides insight into fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention.
33173002	10	111	from	hosts	1994:1998	arg1	absent					1976:1981	absent	1976:1981	absent	1976:1981	Collectively, our work provides insight into fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention.
33173002	10	111	from	hosts	1994:1998	arg1	wall					1930:1933	The cell wall	1921:1933	The cell wall	1921:1933	Collectively, our work provides insight into fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention.
33173002	6	112	theme	biosynthetic	1033:1044	arg1	genes					1046:1050	GAG biosynthetic genes agd3 and ega3	1029:1064	GAG biosynthetic genes agd3 and ega3	1029:1064	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	6	112	theme	biosynthetic	1033:1044	arg1	ega3					1061:1064	ega3	1061:1064	ega3	1061:1064	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	6	112	theme	biosynthetic	1033:1044	arg1	agd3					1052:1055	agd3	1052:1055	agd3	1052:1055	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	6	112	theme	biosynthetic	1033:1044	arg1	stuA					1102:1105	stuA	1102:1105	stuA	1102:1105	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	6	112	theme	biosynthetic	1033:1044	arg1	medA					1093:1096	medA	1093:1096	medA	1093:1096	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	7	113	theme	cell	1327:1330	arg1	biosynthesis					1352:1363	cell wall polysaccharide biosynthesis	1327:1363	cell wall polysaccharide biosynthesis	1327:1363	Thus, SomA plays a direct regulation of both GAG and cell wall polysaccharide biosynthesis.
33173002	12	114	theme	human	2401:2405	arg1	pathogen					2407:2414	the human pathogen	2397:2414	the human pathogen Aspergillus fumigatus We further identified SomA as a master transcription factor playing a dual role in both biofilm formation and cell wall homeostasis	2397:2568	Here, we demonstrate that caspofungin and other cell wall stressors can induce galactosaminogalactan (GAG)-dependent biofilm formation in the human pathogen Aspergillus fumigatus We further identified SomA as a master transcription factor playing a dual role in both biofilm formation and cell wall homeostasis.
33173002	0	115	theme	Transcription	4:16	arg1	Factor					18:23	Transcription Factor	4:23	The Transcription Factor SomA	0:28	The Transcription Factor SomA Synchronously Regulates Biofilm Formation and Cell Wall Homeostasis in Aspergillus fumigatus.
33173002	7	116	theme	GAG	1319:1321	arg1	regulation					1300:1309	a direct regulation	1291:1309	a direct regulation of both GAG and cell wall polysaccharide biosynthesis	1291:1363	Thus, SomA plays a direct regulation of both GAG and cell wall polysaccharide biosynthesis.
33173002	14	117	theme	antifungal	2939:2948	arg1	development					2955:2965	antifungal drug development	2939:2965	antifungal drug development	2939:2965	Collectively, these findings reveal a transcriptional control pathway that integrates biofilm formation and cell wall homeostasis and suggest SomA as an attractive target for antifungal drug development.
33173002	4	118	theme	crucial	778:784	arg1	role					786:789	a crucial role	776:789	a crucial role	776:789	Here, we demonstrate that cell wall stress promotes production of the exopolysaccharide galactosaminogalactan (GAG)-depend biofilm formation in the major fungal pathogen of humans Aspergillus fumigatus and that the transcription factor SomA plays a crucial role in mediating this process.
33173002	8	119	from	function	1504:1511	arg1	development					1524:1534	biofilm development	1516:1534	biofilm development	1516:1534	Consistent with these findings, SomA is required for the maintenance of normal cell wall architecture and compositions in addition to its function in biofilm development.
33173002	9	120	theme	glucose	1583:1589	arg1	uptake					1591:1596	glucose uptake	1583:1596	glucose uptake	1583:1596	Moreover, SomA was found to globally regulate glucose uptake and utilization, as well as amino sugar and nucleotide sugar metabolism, which provides precursors for polysaccharide synthesis.
33173002	6	121	theme	regulators	1082:1091	arg1	regions					1018:1024	the promoter regions	1005:1024	the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA	1005:1105	We identified a novel SomA-binding site in the promoter regions of GAG biosynthetic genes agd3 and ega3, as well as its regulators medA and stuA Strikingly, this SomA-binding site was also found in the upstream regions of genes encoding the cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	12	122	theme	transcription	2477:2489	arg1	factor					2491:2496	a master transcription factor	2468:2496	a master transcription factor playing a dual role in both biofilm formation and cell wall homeostasis	2468:2568	Here, we demonstrate that caspofungin and other cell wall stressors can induce galactosaminogalactan (GAG)-dependent biofilm formation in the human pathogen Aspergillus fumigatus We further identified SomA as a master transcription factor playing a dual role in both biofilm formation and cell wall homeostasis.
33173002	12	122	theme	transcription	2477:2489	arg1	SomA					2460:2463	SomA	2460:2463	SomA	2460:2463	Here, we demonstrate that caspofungin and other cell wall stressors can induce galactosaminogalactan (GAG)-dependent biofilm formation in the human pathogen Aspergillus fumigatus We further identified SomA as a master transcription factor playing a dual role in both biofilm formation and cell wall homeostasis.
33173002	5	123	theme	core	820:823	arg1	genes					844:848	SomA target genes	832:848	SomA target genes	832:848	A core set of SomA target genes were identified by transcriptome sequencing and chromatin immunoprecipitation coupled to sequencing (ChIP-Seq).
33173002	5	123	theme	core	820:823	arg1	set					825:827	A core set	818:827	A core set of SomA target genes	818:848	A core set of SomA target genes were identified by transcriptome sequencing and chromatin immunoprecipitation coupled to sequencing (ChIP-Seq).
33173002	7	124	theme	direct	1293:1298	arg1	regulation					1300:1309	a direct regulation	1291:1309	a direct regulation of both GAG and cell wall polysaccharide biosynthesis	1291:1363	Thus, SomA plays a direct regulation of both GAG and cell wall polysaccharide biosynthesis.
33173002	3	125	theme	biosynthetic	428:439	arg1	pathways					441:448	the biosynthetic pathways	424:448	the biosynthetic pathways governing the production of these polysaccharides	424:498	It is not clear, however, if the biosynthetic pathways governing the production of these polysaccharides are cooperatively regulated.
33173002	4	126	theme	wall	560:563	arg1	stress					565:570	cell wall stress	555:570	cell wall stress	555:570	Here, we demonstrate that cell wall stress promotes production of the exopolysaccharide galactosaminogalactan (GAG)-depend biofilm formation in the major fungal pathogen of humans Aspergillus fumigatus and that the transcription factor SomA plays a crucial role in mediating this process.
33173002	10	127	theme	wall	1864:1867	arg1	homeostasis					1869:1879	cell wall homeostasis	1859:1879	cell wall homeostasis	1859:1879	Collectively, our work provides insight into fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention.
33173002	10	128	theme	cell	1925:1928	arg1	essential					1938:1946	essential	1938:1946	essential	1938:1946	Collectively, our work provides insight into fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention.
33173002	10	128	theme	cell	1925:1928	arg1	wall					1930:1933	The cell wall	1921:1933	The cell wall	1921:1933	Collectively, our work provides insight into fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention.
33173002	10	128	theme	cell	1925:1928	arg1	absent					1976:1981	absent	1976:1981	absent	1976:1981	Collectively, our work provides insight into fungal adaptive mechanisms in response to cell wall stress where biofilm formation and cell wall homeostasis were synchronously regulated.IMPORTANCE The cell wall is essential for fungal viability and is absent from human hosts; thus, drugs disrupting cell wall biosynthesis have gained more attention.
33173002	13	129	theme	β-1,3-glucan	2741:2752	arg1	synthase					2754:2761	β-1,3-glucan synthase	2741:2761	β-1,3-glucan synthase	2741:2761	SomA plays this dual role by direct binding to a conserved motif upstream of GAG biosynthetic genes and genes involved in cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
33173002	4	130	theme	biofilm	652:658	arg1	formation					660:668	the exopolysaccharide galactosaminogalactan (GAG)-depend biofilm formation	595:668	the exopolysaccharide galactosaminogalactan (GAG)-depend biofilm formation	595:668	Here, we demonstrate that cell wall stress promotes production of the exopolysaccharide galactosaminogalactan (GAG)-depend biofilm formation in the major fungal pathogen of humans Aspergillus fumigatus and that the transcription factor SomA plays a crucial role in mediating this process.
33173002	9	131	theme	amino	1626:1630	arg1	sugar					1632:1636	amino sugar and nucleotide sugar metabolism	1626:1668	sugar	1632:1636	Moreover, SomA was found to globally regulate glucose uptake and utilization, as well as amino sugar and nucleotide sugar metabolism, which provides precursors for polysaccharide synthesis.
33173002	5	132	theme	target	837:842	arg1	genes					844:848	SomA target genes	832:848	SomA target genes	832:848	A core set of SomA target genes were identified by transcriptome sequencing and chromatin immunoprecipitation coupled to sequencing (ChIP-Seq).
33173002	13	133	theme	chitin	2719:2724	arg1	synthases					2726:2734	chitin synthases	2719:2734	chitin synthases	2719:2734	SomA plays this dual role by direct binding to a conserved motif upstream of GAG biosynthetic genes and genes involved in cell wall stress sensors, chitin synthases, and β-1,3-glucan synthase.
32416908	0	0	theme	valuable	88:95	arg1	substrates					107:116	valuable UDP sugar substrates	88:116	valuable UDP sugar substrates	88:116	Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substrates.
32416908	7	1	from	embedded	1164:1171	arg1	peptidoglycan					1186:1198	bacterial peptidoglycan	1176:1198	bacterial peptidoglycan	1176:1198	The promiscuity of these enzymes allows for the unique chemical functionality to be embedded in bacterial peptidoglycan both in vitro and in whole bacterial cells for subsequent structural and functional studies of this important biopolymer.
32416908	5	2	theme	muramic	765:771	arg1	derivatives					778:788	UDP-N-acetyl muramic acid derivatives	752:788	UDP-N-acetyl muramic acid derivatives (UDP-NAM)	752:798	A scalable strategy to obtain UDP-N-acetyl muramic acid derivatives (UDP-NAM), the first committed intermediate used solely in peptidoglycan biosynthesis, is described herein.
32416908	5	2	theme	muramic	765:771	arg1	UDP-NAM					791:797	UDP-NAM	791:797	UDP-NAM	791:797	A scalable strategy to obtain UDP-N-acetyl muramic acid derivatives (UDP-NAM), the first committed intermediate used solely in peptidoglycan biosynthesis, is described herein.
32416908	6	3	theme	α-1-phosphate	1023:1035	arg1	transferase					1046:1056	α-1-phosphate uridylyl transferase	1023:1056	α-1-phosphate uridylyl transferase (MurU)	1023:1063	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	6	3	theme	α-1-phosphate	1023:1035	arg1	MurU					1059:1062	MurU	1059:1062	MurU	1059:1062	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	6	4	dep	enzymes	970:976	arg1	NAM					1019:1021	NAM	1019:1021	NAM	1019:1021	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	6	4	dep	enzymes	970:976	arg1	kinase					1001:1006	MurNAc/GlcNAc anomeric kinase	978:1006	MurNAc/GlcNAc anomeric kinase (AmgK)	978:1013	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	6	4	dep	enzymes	970:976	arg1	AmgK					1009:1012	AmgK	1009:1012	AmgK	1009:1012	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	6	4	dep	enzymes	970:976	arg1	transferase					1046:1056	α-1-phosphate uridylyl transferase	1023:1056	α-1-phosphate uridylyl transferase (MurU)	1023:1063	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	6	4	dep	enzymes	970:976	arg1	MurU					1059:1062	MurU	1059:1062	MurU	1059:1062	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	6	4	dep	enzymes	970:976	arg1	enzymes					970:976	the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM	946:1021	the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively	946:1077	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	1	5	theme	essential	156:164	arg1	sugars					145:150	Uridine diphosphate (UDP) sugars	119:150	Uridine diphosphate (UDP) sugars	119:150	Uridine diphosphate (UDP) sugars are essential precursors for glycosylation reactions in all forms of life.
32416908	1	5	theme	essential	156:164	arg1	precursors					166:175	essential precursors	156:175	essential precursors for glycosylation reactions	156:203	Uridine diphosphate (UDP) sugars are essential precursors for glycosylation reactions in all forms of life.
32416908	0	6	theme	sugar	101:105	arg1	substrates					107:116	valuable UDP sugar substrates	88:116	valuable UDP sugar substrates	88:116	Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substrates.
32416908	0	7	from	Utility	0:6	arg1	synthesis					75:83	the chemoenzymatic synthesis	56:83	the chemoenzymatic synthesis of valuable UDP sugar substrates	56:116	Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substrates.
32416908	7	8	theme	unique	1128:1133	arg1	functionality					1144:1156	the unique chemical functionality	1124:1156	the unique chemical functionality	1124:1156	The promiscuity of these enzymes allows for the unique chemical functionality to be embedded in bacterial peptidoglycan both in vitro and in whole bacterial cells for subsequent structural and functional studies of this important biopolymer.
32416908	3	9	theme	negative	363:370	arg1	charge					386:391	negative physiological charge	363:391	negative physiological charge	363:391	While the stereochemistry and negative physiological charge of UDP-sugars are essential for their biochemical function in the cell, these characteristics make them challenging molecules to synthesize and purify on scale in the laboratory.
32416908	7	10	from	peptidoglycan	1186:1198	arg1	promiscuity					1084:1094	The promiscuity	1080:1094	The promiscuity of these enzymes	1080:1111	The promiscuity of these enzymes allows for the unique chemical functionality to be embedded in bacterial peptidoglycan both in vitro and in whole bacterial cells for subsequent structural and functional studies of this important biopolymer.
32416908	7	10	from	peptidoglycan	1186:1198	arg1	embedded					1164:1171	embedded	1164:1171	embedded	1164:1171	The promiscuity of these enzymes allows for the unique chemical functionality to be embedded in bacterial peptidoglycan both in vitro and in whole bacterial cells for subsequent structural and functional studies of this important biopolymer.
32416908	7	11	theme	chemical	1135:1142	arg1	functionality					1144:1156	the unique chemical functionality	1124:1156	the unique chemical functionality	1124:1156	The promiscuity of these enzymes allows for the unique chemical functionality to be embedded in bacterial peptidoglycan both in vitro and in whole bacterial cells for subsequent structural and functional studies of this important biopolymer.
32416908	7	12	theme	functional	1273:1282	arg1	studies					1284:1290	subsequent structural and functional studies	1247:1290	subsequent structural and functional studies of this important biopolymer	1247:1319	The promiscuity of these enzymes allows for the unique chemical functionality to be embedded in bacterial peptidoglycan both in vitro and in whole bacterial cells for subsequent structural and functional studies of this important biopolymer.
32416908	6	13	theme	uridylyl	1037:1044	arg1	transferase					1046:1056	α-1-phosphate uridylyl transferase	1023:1056	α-1-phosphate uridylyl transferase (MurU)	1023:1063	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	6	13	theme	uridylyl	1037:1044	arg1	MurU					1059:1062	MurU	1059:1062	MurU	1059:1062	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	5	14	theme	UDP-N-acetyl	752:763	arg1	derivatives					778:788	UDP-N-acetyl muramic acid derivatives	752:788	UDP-N-acetyl muramic acid derivatives (UDP-NAM)	752:798	A scalable strategy to obtain UDP-N-acetyl muramic acid derivatives (UDP-NAM), the first committed intermediate used solely in peptidoglycan biosynthesis, is described herein.
32416908	5	14	theme	UDP-N-acetyl	752:763	arg1	UDP-NAM					791:797	UDP-NAM	791:797	UDP-NAM	791:797	A scalable strategy to obtain UDP-N-acetyl muramic acid derivatives (UDP-NAM), the first committed intermediate used solely in peptidoglycan biosynthesis, is described herein.
32416908	7	15	theme	biopolymer	1310:1319	arg1	studies					1284:1290	subsequent structural and functional studies	1247:1290	subsequent structural and functional studies of this important biopolymer	1247:1319	The promiscuity of these enzymes allows for the unique chemical functionality to be embedded in bacterial peptidoglycan both in vitro and in whole bacterial cells for subsequent structural and functional studies of this important biopolymer.
32416908	2	16	theme	UDP	277:279	arg1	donor					281:285	the UDP donor	273:285	the UDP donor	273:285	Reactions that transfer the carbohydrate from the UDP donor are catalyzed by glycosyltransferases (Gtfs).
32416908	3	17	theme	biochemical	431:441	arg1	function					443:450	their biochemical function	425:450	their biochemical function in the cell	425:462	While the stereochemistry and negative physiological charge of UDP-sugars are essential for their biochemical function in the cell, these characteristics make them challenging molecules to synthesize and purify on scale in the laboratory.
32416908	4	18	theme	building	669:676	arg1	UDP-sugars					653:662	muramyl UDP-sugars	645:662	muramyl UDP-sugars	645:662	This chapter focuses on the utilization of a chemoenzymatic synthesis of muramyl UDP-sugars, key building blocks in the bacterial cell peptidoglycan.
32416908	4	18	theme	building	669:676	arg1	blocks					678:683	key building blocks	665:683	key building blocks in the bacterial cell peptidoglycan	665:719	This chapter focuses on the utilization of a chemoenzymatic synthesis of muramyl UDP-sugars, key building blocks in the bacterial cell peptidoglycan.
32416908	0	19	theme	substrates	107:116	arg1	synthesis					75:83	the chemoenzymatic synthesis	56:83	the chemoenzymatic synthesis of valuable UDP sugar substrates	56:116	Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substrates.
32416908	4	20	theme	key	665:667	arg1	UDP-sugars					653:662	muramyl UDP-sugars	645:662	muramyl UDP-sugars	645:662	This chapter focuses on the utilization of a chemoenzymatic synthesis of muramyl UDP-sugars, key building blocks in the bacterial cell peptidoglycan.
32416908	4	20	theme	key	665:667	arg1	blocks					678:683	key building blocks	665:683	key building blocks in the bacterial cell peptidoglycan	665:719	This chapter focuses on the utilization of a chemoenzymatic synthesis of muramyl UDP-sugars, key building blocks in the bacterial cell peptidoglycan.
32416908	5	21	theme	committed	811:819	arg1	intermediate					821:832	the first committed intermediate	801:832	the first committed intermediate used solely in peptidoglycan biosynthesis	801:874	A scalable strategy to obtain UDP-N-acetyl muramic acid derivatives (UDP-NAM), the first committed intermediate used solely in peptidoglycan biosynthesis, is described herein.
32416908	5	21	theme	committed	811:819	arg1	strategy					733:740	A scalable strategy	722:740	A scalable strategy to obtain UDP-N-acetyl muramic acid derivatives (UDP-NAM)	722:798	A scalable strategy to obtain UDP-N-acetyl muramic acid derivatives (UDP-NAM), the first committed intermediate used solely in peptidoglycan biosynthesis, is described herein.
32416908	6	22	theme	anomeric	992:999	arg1	enzymes					970:976	the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM	946:1021	the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively	946:1077	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	6	22	theme	anomeric	992:999	arg1	kinase					1001:1006	MurNAc/GlcNAc anomeric kinase	978:1006	MurNAc/GlcNAc anomeric kinase (AmgK)	978:1013	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	6	22	theme	anomeric	992:999	arg1	AmgK					1009:1012	AmgK	1009:1012	AmgK	1009:1012	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	7	23	theme	enzymes	1105:1111	arg1	promiscuity					1084:1094	The promiscuity	1080:1094	The promiscuity of these enzymes	1080:1111	The promiscuity of these enzymes allows for the unique chemical functionality to be embedded in bacterial peptidoglycan both in vitro and in whole bacterial cells for subsequent structural and functional studies of this important biopolymer.
32416908	7	23	theme	enzymes	1105:1111	arg1	embedded					1164:1171	embedded	1164:1171	embedded	1164:1171	The promiscuity of these enzymes allows for the unique chemical functionality to be embedded in bacterial peptidoglycan both in vitro and in whole bacterial cells for subsequent structural and functional studies of this important biopolymer.
32416908	7	24	theme	subsequent	1247:1256	arg1	studies					1284:1290	subsequent structural and functional studies	1247:1290	subsequent structural and functional studies of this important biopolymer	1247:1319	The promiscuity of these enzymes allows for the unique chemical functionality to be embedded in bacterial peptidoglycan both in vitro and in whole bacterial cells for subsequent structural and functional studies of this important biopolymer.
32416908	7	25	theme	important	1300:1308	arg1	biopolymer					1310:1319	this important biopolymer	1295:1319	this important biopolymer	1295:1319	The promiscuity of these enzymes allows for the unique chemical functionality to be embedded in bacterial peptidoglycan both in vitro and in whole bacterial cells for subsequent structural and functional studies of this important biopolymer.
32416908	1	26	theme	glycosylation	181:193	arg1	reactions					195:203	glycosylation reactions	181:203	glycosylation reactions	181:203	Uridine diphosphate (UDP) sugars are essential precursors for glycosylation reactions in all forms of life.
32416908	3	27	theme	UDP-sugars	396:405	arg1	stereochemistry					343:357	stereochemistry	343:357	stereochemistry	343:357	While the stereochemistry and negative physiological charge of UDP-sugars are essential for their biochemical function in the cell, these characteristics make them challenging molecules to synthesize and purify on scale in the laboratory.
32416908	3	27	theme	UDP-sugars	396:405	arg1	charge					386:391	negative physiological charge	363:391	negative physiological charge	363:391	While the stereochemistry and negative physiological charge of UDP-sugars are essential for their biochemical function in the cell, these characteristics make them challenging molecules to synthesize and purify on scale in the laboratory.
32416908	0	28	theme	peptidoglycan	21:33	arg1	enzymes					45:51	bacterial peptidoglycan recycling enzymes	11:51	bacterial peptidoglycan recycling enzymes	11:51	Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substrates.
32416908	4	29	theme	muramyl	645:651	arg1	UDP-sugars					653:662	muramyl UDP-sugars	645:662	muramyl UDP-sugars	645:662	This chapter focuses on the utilization of a chemoenzymatic synthesis of muramyl UDP-sugars, key building blocks in the bacterial cell peptidoglycan.
32416908	4	29	theme	muramyl	645:651	arg1	blocks					678:683	key building blocks	665:683	key building blocks in the bacterial cell peptidoglycan	665:719	This chapter focuses on the utilization of a chemoenzymatic synthesis of muramyl UDP-sugars, key building blocks in the bacterial cell peptidoglycan.
32416908	0	30	theme	bacterial	11:19	arg1	enzymes					45:51	bacterial peptidoglycan recycling enzymes	11:51	bacterial peptidoglycan recycling enzymes	11:51	Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substrates.
32416908	4	31	theme	cell	702:705	arg1	peptidoglycan					707:719	the bacterial cell peptidoglycan	688:719	the bacterial cell peptidoglycan	688:719	This chapter focuses on the utilization of a chemoenzymatic synthesis of muramyl UDP-sugars, key building blocks in the bacterial cell peptidoglycan.
32416908	5	32	theme	peptidoglycan	849:861	arg1	biosynthesis					863:874	peptidoglycan biosynthesis	849:874	peptidoglycan biosynthesis	849:874	A scalable strategy to obtain UDP-N-acetyl muramic acid derivatives (UDP-NAM), the first committed intermediate used solely in peptidoglycan biosynthesis, is described herein.
32416908	0	33	theme	enzymes	45:51	arg1	Utility					0:6	Utility	0:6	Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substrates.	0:117	Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substrates.
32416908	4	34	from	blocks	678:683	arg1	peptidoglycan					707:719	the bacterial cell peptidoglycan	688:719	the bacterial cell peptidoglycan	688:719	This chapter focuses on the utilization of a chemoenzymatic synthesis of muramyl UDP-sugars, key building blocks in the bacterial cell peptidoglycan.
32416908	5	35	theme	scalable	724:731	arg1	intermediate					821:832	the first committed intermediate	801:832	the first committed intermediate used solely in peptidoglycan biosynthesis	801:874	A scalable strategy to obtain UDP-N-acetyl muramic acid derivatives (UDP-NAM), the first committed intermediate used solely in peptidoglycan biosynthesis, is described herein.
32416908	5	35	theme	scalable	724:731	arg1	strategy					733:740	A scalable strategy	722:740	A scalable strategy to obtain UDP-N-acetyl muramic acid derivatives (UDP-NAM)	722:798	A scalable strategy to obtain UDP-N-acetyl muramic acid derivatives (UDP-NAM), the first committed intermediate used solely in peptidoglycan biosynthesis, is described herein.
32416908	0	36	theme	recycling	35:43	arg1	enzymes					45:51	bacterial peptidoglycan recycling enzymes	11:51	bacterial peptidoglycan recycling enzymes	11:51	Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substrates.
32416908	6	37	theme	MurNAc/GlcNAc	978:990	arg1	enzymes					970:976	the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM	946:1021	the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively	946:1077	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	6	37	theme	MurNAc/GlcNAc	978:990	arg1	kinase					1001:1006	MurNAc/GlcNAc anomeric kinase	978:1006	MurNAc/GlcNAc anomeric kinase (AmgK)	978:1013	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	6	37	theme	MurNAc/GlcNAc	978:990	arg1	AmgK					1009:1012	AmgK	1009:1012	AmgK	1009:1012	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	7	38	theme	bacterial	1176:1184	arg1	peptidoglycan					1186:1198	bacterial peptidoglycan	1176:1198	bacterial peptidoglycan	1176:1198	The promiscuity of these enzymes allows for the unique chemical functionality to be embedded in bacterial peptidoglycan both in vitro and in whole bacterial cells for subsequent structural and functional studies of this important biopolymer.
32416908	3	39	theme	physiological	372:384	arg1	charge					386:391	negative physiological charge	363:391	negative physiological charge	363:391	While the stereochemistry and negative physiological charge of UDP-sugars are essential for their biochemical function in the cell, these characteristics make them challenging molecules to synthesize and purify on scale in the laboratory.
32416908	4	40	theme	UDP-sugars	653:662	arg1	synthesis					632:640	a chemoenzymatic synthesis	615:640	a chemoenzymatic synthesis of muramyl UDP-sugars, key building blocks in the bacterial cell peptidoglycan	615:719	This chapter focuses on the utilization of a chemoenzymatic synthesis of muramyl UDP-sugars, key building blocks in the bacterial cell peptidoglycan.
32416908	7	41	theme	whole	1221:1225	arg1	cells					1237:1241	whole bacterial cells	1221:1241	whole bacterial cells for subsequent structural and functional studies of this important biopolymer	1221:1319	The promiscuity of these enzymes allows for the unique chemical functionality to be embedded in bacterial peptidoglycan both in vitro and in whole bacterial cells for subsequent structural and functional studies of this important biopolymer.
32416908	5	42	dep	strategy	733:740	arg1	obtain					745:750	obtain	745:750	to obtain UDP-N-acetyl muramic acid derivatives (UDP-NAM)	742:798	A scalable strategy to obtain UDP-N-acetyl muramic acid derivatives (UDP-NAM), the first committed intermediate used solely in peptidoglycan biosynthesis, is described herein.
32416908	1	43	theme	Uridine	119:125	arg1	sugars					145:150	Uridine diphosphate (UDP) sugars	119:150	Uridine diphosphate (UDP) sugars	119:150	Uridine diphosphate (UDP) sugars are essential precursors for glycosylation reactions in all forms of life.
32416908	1	43	theme	Uridine	119:125	arg1	precursors					166:175	essential precursors	156:175	essential precursors for glycosylation reactions	156:203	Uridine diphosphate (UDP) sugars are essential precursors for glycosylation reactions in all forms of life.
32416908	6	44	theme	recycling	960:968	arg1	enzymes					970:976	the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM	946:1021	the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively	946:1077	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	6	44	theme	recycling	960:968	arg1	NAM					1019:1021	NAM	1019:1021	NAM	1019:1021	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	6	44	theme	recycling	960:968	arg1	kinase					1001:1006	MurNAc/GlcNAc anomeric kinase	978:1006	MurNAc/GlcNAc anomeric kinase (AmgK)	978:1013	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	3	45	dep	stereochemistry	343:357	arg1	the					339:341	the	339:341	the	339:341	While the stereochemistry and negative physiological charge of UDP-sugars are essential for their biochemical function in the cell, these characteristics make them challenging molecules to synthesize and purify on scale in the laboratory.
32416908	7	46	theme	bacterial	1227:1235	arg1	cells					1237:1241	whole bacterial cells	1221:1241	whole bacterial cells for subsequent structural and functional studies of this important biopolymer	1221:1319	The promiscuity of these enzymes allows for the unique chemical functionality to be embedded in bacterial peptidoglycan both in vitro and in whole bacterial cells for subsequent structural and functional studies of this important biopolymer.
32416908	1	47	theme	diphosphate	127:137	arg1	sugars					145:150	Uridine diphosphate (UDP) sugars	119:150	Uridine diphosphate (UDP) sugars	119:150	Uridine diphosphate (UDP) sugars are essential precursors for glycosylation reactions in all forms of life.
32416908	1	47	theme	diphosphate	127:137	arg1	precursors					166:175	essential precursors	156:175	essential precursors for glycosylation reactions	156:203	Uridine diphosphate (UDP) sugars are essential precursors for glycosylation reactions in all forms of life.
32416908	6	48	theme	wall	955:958	arg1	enzymes					970:976	the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM	946:1021	the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively	946:1077	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	6	48	theme	wall	955:958	arg1	NAM					1019:1021	NAM	1019:1021	NAM	1019:1021	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	6	48	theme	wall	955:958	arg1	kinase					1001:1006	MurNAc/GlcNAc anomeric kinase	978:1006	MurNAc/GlcNAc anomeric kinase (AmgK)	978:1013	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	1	49	theme	life	221:224	arg1	forms					212:216	all forms	208:216	all forms of life	208:224	Uridine diphosphate (UDP) sugars are essential precursors for glycosylation reactions in all forms of life.
32416908	1	50	theme	UDP	140:142	arg1	sugars					145:150	Uridine diphosphate (UDP) sugars	119:150	Uridine diphosphate (UDP) sugars	119:150	Uridine diphosphate (UDP) sugars are essential precursors for glycosylation reactions in all forms of life.
32416908	1	50	theme	UDP	140:142	arg1	precursors					166:175	essential precursors	156:175	essential precursors for glycosylation reactions	156:203	Uridine diphosphate (UDP) sugars are essential precursors for glycosylation reactions in all forms of life.
32416908	5	51	theme	acid	773:776	arg1	derivatives					778:788	UDP-N-acetyl muramic acid derivatives	752:788	UDP-N-acetyl muramic acid derivatives (UDP-NAM)	752:798	A scalable strategy to obtain UDP-N-acetyl muramic acid derivatives (UDP-NAM), the first committed intermediate used solely in peptidoglycan biosynthesis, is described herein.
32416908	5	51	theme	acid	773:776	arg1	UDP-NAM					791:797	UDP-NAM	791:797	UDP-NAM	791:797	A scalable strategy to obtain UDP-N-acetyl muramic acid derivatives (UDP-NAM), the first committed intermediate used solely in peptidoglycan biosynthesis, is described herein.
32416908	3	52	from	function	443:450	arg1	cell					459:462	the cell	455:462	the cell	455:462	While the stereochemistry and negative physiological charge of UDP-sugars are essential for their biochemical function in the cell, these characteristics make them challenging molecules to synthesize and purify on scale in the laboratory.
32416908	4	53	theme	bacterial	692:700	arg1	peptidoglycan					707:719	the bacterial cell peptidoglycan	688:719	the bacterial cell peptidoglycan	688:719	This chapter focuses on the utilization of a chemoenzymatic synthesis of muramyl UDP-sugars, key building blocks in the bacterial cell peptidoglycan.
32416908	7	54	theme	structural	1258:1267	arg1	studies					1284:1290	subsequent structural and functional studies	1247:1290	subsequent structural and functional studies of this important biopolymer	1247:1319	The promiscuity of these enzymes allows for the unique chemical functionality to be embedded in bacterial peptidoglycan both in vitro and in whole bacterial cells for subsequent structural and functional studies of this important biopolymer.
32416908	4	55	theme	synthesis	632:640	arg1	utilization					600:610	the utilization	596:610	the utilization of a chemoenzymatic synthesis of muramyl UDP-sugars, key building blocks in the bacterial cell peptidoglycan	596:719	This chapter focuses on the utilization of a chemoenzymatic synthesis of muramyl UDP-sugars, key building blocks in the bacterial cell peptidoglycan.
32416908	0	56	theme	chemoenzymatic	60:73	arg1	synthesis					75:83	the chemoenzymatic synthesis	56:83	the chemoenzymatic synthesis of valuable UDP sugar substrates	56:116	Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substrates.
32416908	6	57	theme	cell	950:953	arg1	enzymes					970:976	the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM	946:1021	the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively	946:1077	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	6	57	theme	cell	950:953	arg1	NAM					1019:1021	NAM	1019:1021	NAM	1019:1021	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	6	57	theme	cell	950:953	arg1	kinase					1001:1006	MurNAc/GlcNAc anomeric kinase	978:1006	MurNAc/GlcNAc anomeric kinase (AmgK)	978:1013	This methodology utilizes two enzymes involving the cell wall recycling enzymes MurNAc/GlcNAc anomeric kinase (AmgK) and NAM α-1-phosphate uridylyl transferase (MurU), respectively.
32416908	4	58	theme	chemoenzymatic	617:630	arg1	synthesis					632:640	a chemoenzymatic synthesis	615:640	a chemoenzymatic synthesis of muramyl UDP-sugars, key building blocks in the bacterial cell peptidoglycan	615:719	This chapter focuses on the utilization of a chemoenzymatic synthesis of muramyl UDP-sugars, key building blocks in the bacterial cell peptidoglycan.
32416908	1	59	from	precursors	166:175	arg1	forms					212:216	all forms	208:216	all forms of life	208:224	Uridine diphosphate (UDP) sugars are essential precursors for glycosylation reactions in all forms of life.
32416908	0	60	theme	UDP	97:99	arg1	substrates					107:116	valuable UDP sugar substrates	88:116	valuable UDP sugar substrates	88:116	Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substrates.
33662950	8	0	dep	low	1696:1698	arg1	1 kPa					1716:1720	1 kPa	1716:1720	1 kPa	1716:1720	Shear stresses were relatively low while printing (1 kPa) as a result of the shear thinning property of the material, which supported cell viability during extrusion.
33662950	1	1	from	environment	333:343	arg1	tissues					348:354	tissues	348:354	tissues	348:354	3D bioprinting is a recent technique that can create complex cell seeded scaffolds and therefore holds great promise to revolutionize the biomedical sector by combining materials and structures that more closely mimic the 3D cell environment in tissues.
33662950	8	2	theme	material	1773:1780	arg1	property					1757:1764	the shear thinning property	1738:1764	the shear thinning property of the material	1738:1780	Shear stresses were relatively low while printing (1 kPa) as a result of the shear thinning property of the material, which supported cell viability during extrusion.
33662950	5	3	theme	%	1211:1211	arg1	concentrations					1191:1204	high gel concentrations	1182:1204	high gel concentrations (5+5 % XGMA-GelMA)	1182:1223	This was confirmed in printing studies, with measured normalized strand widths of 1.2 obtained for high gel concentrations (5+5 % XGMA-GelMA).
33662950	5	3	theme	%	1211:1211	arg1	XGMA-GelMA					1213:1222	5+5 % XGMA-GelMA	1207:1222	5+5 % XGMA-GelMA	1207:1222	This was confirmed in printing studies, with measured normalized strand widths of 1.2 obtained for high gel concentrations (5+5 % XGMA-GelMA).
33662950	10	4	theme	bio-functional	2231:2244	arg1	bioink					2246:2251	a bio-functional bioink	2229:2251	a bio-functional bioink	2229:2251	In conclusion, stability and formulation of a XGMA-GelMA shear thinning composite hydrogel has been optimized to create a bio-functional bioink, with improved printability, andin vitroculture stabilityviasecondary photo-induced cross-linking, making this composite a promising bioink for 3D bioprinting.
33662950	3	5	theme	bio-functional	697:710	arg1	material					712:719	a bio-functional material	695:719	a bio-functional material such as gelatin methacryloyl (GelMa)	695:756	We propose that bioinks with intrinsic self-assembling and shear thinning properties, such as xanthan gum, can be methacrylated (XGMA) and combined with a bio-functional material such as gelatin methacryloyl (GelMa) to create a stable, cell-interactive bioink with improved properties for 3D bioprinting.
33662950	8	6	theme	cell	1799:1802	arg1	viability					1804:1812	cell viability	1799:1812	cell viability	1799:1812	Shear stresses were relatively low while printing (1 kPa) as a result of the shear thinning property of the material, which supported cell viability during extrusion.
33662950	3	7	theme	self-assembling	581:595	arg1	gum					644:646	xanthan gum	636:646	xanthan gum	636:646	We propose that bioinks with intrinsic self-assembling and shear thinning properties, such as xanthan gum, can be methacrylated (XGMA) and combined with a bio-functional material such as gelatin methacryloyl (GelMa) to create a stable, cell-interactive bioink with improved properties for 3D bioprinting.
33662950	3	7	theme	self-assembling	581:595	arg1	properties					616:625	intrinsic self-assembling and shear thinning properties	571:625	intrinsic self-assembling and shear thinning properties	571:625	We propose that bioinks with intrinsic self-assembling and shear thinning properties, such as xanthan gum, can be methacrylated (XGMA) and combined with a bio-functional material such as gelatin methacryloyl (GelMa) to create a stable, cell-interactive bioink with improved properties for 3D bioprinting.
33662950	6	8	with	tuning	1306:1311	arg1	stiffness					1363:1371	stiffness	1363:1371	stiffness	1363:1371	Furthermore, the introduction of a secondary photo-cross-linking method allowed tuning of the mechanical properties of the hydrogel with stiffness between 15 and 30 kPa, as well as improving the stability of the hydrogel with retention of 75 % of its mass after 90 d.
33662950	6	9	theme	photo-cross-linking	1271:1289	arg1	method					1291:1296	a secondary photo-cross-linking method	1259:1296	a secondary photo-cross-linking method	1259:1296	Furthermore, the introduction of a secondary photo-cross-linking method allowed tuning of the mechanical properties of the hydrogel with stiffness between 15 and 30 kPa, as well as improving the stability of the hydrogel with retention of 75 % of its mass after 90 d.
33662950	10	10	theme	improved	2259:2266	arg1	printability					2268:2279	improved printability	2259:2279	improved printability	2259:2279	In conclusion, stability and formulation of a XGMA-GelMA shear thinning composite hydrogel has been optimized to create a bio-functional bioink, with improved printability, andin vitroculture stabilityviasecondary photo-induced cross-linking, making this composite a promising bioink for 3D bioprinting.
33662950	9	11	theme	printed	1841:1847	arg1	hydrogels					1849:1857	printed hydrogels	1841:1857	printed hydrogels	1841:1857	Finally, printed hydrogels retained high cell viability for lower gel concentrations, and showed improved cell viability for more concentrated hydrogels when compared to cells cultured in bulk hydrogels, presumably due to improved nutrient/oxygen diffusion and cell migration.
33662950	3	12	theme	gelatin	729:735	arg1	GelMa					751:755	GelMa	751:755	GelMa	751:755	We propose that bioinks with intrinsic self-assembling and shear thinning properties, such as xanthan gum, can be methacrylated (XGMA) and combined with a bio-functional material such as gelatin methacryloyl (GelMa) to create a stable, cell-interactive bioink with improved properties for 3D bioprinting.
33662950	3	12	theme	gelatin	729:735	arg1	methacryloyl					737:748	gelatin methacryloyl	729:748	gelatin methacryloyl (GelMa)	729:756	We propose that bioinks with intrinsic self-assembling and shear thinning properties, such as xanthan gum, can be methacrylated (XGMA) and combined with a bio-functional material such as gelatin methacryloyl (GelMa) to create a stable, cell-interactive bioink with improved properties for 3D bioprinting.
33662950	3	13	dep	stable	770:775	arg1	cell-interactive					778:793	cell-interactive	778:793	cell-interactive	778:793	We propose that bioinks with intrinsic self-assembling and shear thinning properties, such as xanthan gum, can be methacrylated (XGMA) and combined with a bio-functional material such as gelatin methacryloyl (GelMa) to create a stable, cell-interactive bioink with improved properties for 3D bioprinting.
33662950	7	14	theme	cell	1582:1585	arg1	spreading					1587:1595	cell spreading	1582:1595	cell spreading	1582:1595	The hydrogel was shown to be biocompatible and bio-active with 97 % cell viability, and cell spreading after 7 d of culture for low gel concentrations (3+3 % XGMA-GelMA).
33662950	6	15	theme	mechanical	1320:1329	arg1	properties					1331:1340	the mechanical properties	1316:1340	the mechanical properties of the hydrogel	1316:1356	Furthermore, the introduction of a secondary photo-cross-linking method allowed tuning of the mechanical properties of the hydrogel with stiffness between 15 and 30 kPa, as well as improving the stability of the hydrogel with retention of 75 % of its mass after 90 d.
33662950	4	16	theme	high	895:898	arg1	shear					900:904	high shear	895:904	high shear	895:904	These biomaterials have reduced viscosity under high shear and recover their viscosity rapidly after the shear is removed, retaining their shape, which translates to easier extrusion whilst maintaining accurate fidelity after printing.
33662950	9	17	theme	cell	1873:1876	arg1	viability					1878:1886	high cell viability	1868:1886	high cell viability	1868:1886	Finally, printed hydrogels retained high cell viability for lower gel concentrations, and showed improved cell viability for more concentrated hydrogels when compared to cells cultured in bulk hydrogels, presumably due to improved nutrient/oxygen diffusion and cell migration.
33662950	7	18	theme	cell	1562:1565	arg1	viability					1567:1575	cell viability	1562:1575	cell viability	1562:1575	The hydrogel was shown to be biocompatible and bio-active with 97 % cell viability, and cell spreading after 7 d of culture for low gel concentrations (3+3 % XGMA-GelMA).
33662950	3	19	theme	xanthan	636:642	arg1	gum					644:646	xanthan gum	636:646	xanthan gum	636:646	We propose that bioinks with intrinsic self-assembling and shear thinning properties, such as xanthan gum, can be methacrylated (XGMA) and combined with a bio-functional material such as gelatin methacryloyl (GelMa) to create a stable, cell-interactive bioink with improved properties for 3D bioprinting.
33662950	3	20	theme	3D	831:832	arg1	bioprinting					834:844	3D bioprinting	831:844	3D bioprinting	831:844	We propose that bioinks with intrinsic self-assembling and shear thinning properties, such as xanthan gum, can be methacrylated (XGMA) and combined with a bio-functional material such as gelatin methacryloyl (GelMa) to create a stable, cell-interactive bioink with improved properties for 3D bioprinting.
33662950	7	21	theme	97	1557:1558	arg1	%					1560:1560	%	1560:1560	%	1560:1560	The hydrogel was shown to be biocompatible and bio-active with 97 % cell viability, and cell spreading after 7 d of culture for low gel concentrations (3+3 % XGMA-GelMA).
33662950	9	22	theme	gel	1898:1900	arg1	concentrations					1902:1915	lower gel concentrations	1892:1915	lower gel concentrations	1892:1915	Finally, printed hydrogels retained high cell viability for lower gel concentrations, and showed improved cell viability for more concentrated hydrogels when compared to cells cultured in bulk hydrogels, presumably due to improved nutrient/oxygen diffusion and cell migration.
33662950	3	23	theme	stable	770:775	arg1	bioink					795:800	a stable, cell-interactive bioink	768:800	a stable, cell-interactive bioink with improved properties for 3D bioprinting	768:844	We propose that bioinks with intrinsic self-assembling and shear thinning properties, such as xanthan gum, can be methacrylated (XGMA) and combined with a bio-functional material such as gelatin methacryloyl (GelMa) to create a stable, cell-interactive bioink with improved properties for 3D bioprinting.
33662950	0	24	theme	xanthan-gelatin	69:83	arg1	bioink					95:100	xanthan-gelatin composite bioink	69:100	xanthan-gelatin composite bioink	69:100	Printability and bio-functionality of a shear thinning methacrylated xanthan-gelatin composite bioink.
33662950	1	25	theme	complex	156:162	arg1	cell					164:167	complex cell	156:167	complex cell	156:167	3D bioprinting is a recent technique that can create complex cell seeded scaffolds and therefore holds great promise to revolutionize the biomedical sector by combining materials and structures that more closely mimic the 3D cell environment in tissues.
33662950	7	26	theme	%	1650:1650	arg1	XGMA-GelMA					1652:1661	3+3 % XGMA-GelMA	1646:1661	3+3 % XGMA-GelMA	1646:1661	The hydrogel was shown to be biocompatible and bio-active with 97 % cell viability, and cell spreading after 7 d of culture for low gel concentrations (3+3 % XGMA-GelMA).
33662950	7	26	theme	%	1650:1650	arg1	concentrations					1630:1643	low gel concentrations	1622:1643	low gel concentrations (3+3 % XGMA-GelMA)	1622:1662	The hydrogel was shown to be biocompatible and bio-active with 97 % cell viability, and cell spreading after 7 d of culture for low gel concentrations (3+3 % XGMA-GelMA).
33662950	10	27	theme	photo-induced	2323:2335	arg1	cross-linking					2337:2349	andin vitroculture stabilityviasecondary photo-induced cross-linking	2282:2349	andin vitroculture stabilityviasecondary photo-induced cross-linking	2282:2349	In conclusion, stability and formulation of a XGMA-GelMA shear thinning composite hydrogel has been optimized to create a bio-functional bioink, with improved printability, andin vitroculture stabilityviasecondary photo-induced cross-linking, making this composite a promising bioink for 3D bioprinting.
33662950	5	28	theme	measured	1128:1135	arg1	widths					1155:1160	measured normalized strand widths	1128:1160	measured normalized strand widths of 1.2 obtained for high gel concentrations (5+5 % XGMA-GelMA)	1128:1223	This was confirmed in printing studies, with measured normalized strand widths of 1.2 obtained for high gel concentrations (5+5 % XGMA-GelMA).
33662950	1	29	theme	seeded	169:174	arg1	scaffolds					176:184	complex cell seeded scaffolds	156:184	complex cell seeded scaffolds	156:184	3D bioprinting is a recent technique that can create complex cell seeded scaffolds and therefore holds great promise to revolutionize the biomedical sector by combining materials and structures that more closely mimic the 3D cell environment in tissues.
33662950	2	30	theme	interactions	528:539	arg1	issues					471:476	issues	471:476	issues of printability, stability, or poor cell-material interactions	471:539	The most commonly used biomaterials for printing are hydrogels, however, many of the hydrogels used still present issues of printability, stability, or poor cell-material interactions.
33662950	7	31	theme	gel	1626:1628	arg1	XGMA-GelMA					1652:1661	3+3 % XGMA-GelMA	1646:1661	3+3 % XGMA-GelMA	1646:1661	The hydrogel was shown to be biocompatible and bio-active with 97 % cell viability, and cell spreading after 7 d of culture for low gel concentrations (3+3 % XGMA-GelMA).
33662950	7	31	theme	gel	1626:1628	arg1	concentrations					1630:1643	low gel concentrations	1622:1643	low gel concentrations (3+3 % XGMA-GelMA)	1622:1662	The hydrogel was shown to be biocompatible and bio-active with 97 % cell viability, and cell spreading after 7 d of culture for low gel concentrations (3+3 % XGMA-GelMA).
33662950	2	32	theme	poor	509:512	arg1	interactions					528:539	poor cell-material interactions	509:539	poor cell-material interactions	509:539	The most commonly used biomaterials for printing are hydrogels, however, many of the hydrogels used still present issues of printability, stability, or poor cell-material interactions.
33662950	9	33	theme	cell	1938:1941	arg1	viability					1943:1951	improved cell viability	1929:1951	improved cell viability	1929:1951	Finally, printed hydrogels retained high cell viability for lower gel concentrations, and showed improved cell viability for more concentrated hydrogels when compared to cells cultured in bulk hydrogels, presumably due to improved nutrient/oxygen diffusion and cell migration.
33662950	6	34	theme	hydrogel	1349:1356	arg1	properties					1331:1340	the mechanical properties	1316:1340	the mechanical properties of the hydrogel	1316:1356	Furthermore, the introduction of a secondary photo-cross-linking method allowed tuning of the mechanical properties of the hydrogel with stiffness between 15 and 30 kPa, as well as improving the stability of the hydrogel with retention of 75 % of its mass after 90 d.
33662950	5	35	theme	printing	1105:1112	arg1	studies					1114:1120	printing studies	1105:1120	printing studies	1105:1120	This was confirmed in printing studies, with measured normalized strand widths of 1.2 obtained for high gel concentrations (5+5 % XGMA-GelMA).
33662950	8	36	theme	shear	1742:1746	arg1	property					1757:1764	the shear thinning property	1738:1764	the shear thinning property of the material	1738:1780	Shear stresses were relatively low while printing (1 kPa) as a result of the shear thinning property of the material, which supported cell viability during extrusion.
33662950	10	37	theme	3D	2397:2398	arg1	bioprinting					2400:2410	3D bioprinting	2397:2410	3D bioprinting	2397:2410	In conclusion, stability and formulation of a XGMA-GelMA shear thinning composite hydrogel has been optimized to create a bio-functional bioink, with improved printability, andin vitroculture stabilityviasecondary photo-induced cross-linking, making this composite a promising bioink for 3D bioprinting.
33662950	10	38	theme	thinning	2172:2179	arg1	hydrogel					2191:2198	a XGMA-GelMA shear thinning composite hydrogel	2153:2198	a XGMA-GelMA shear thinning composite hydrogel	2153:2198	In conclusion, stability and formulation of a XGMA-GelMA shear thinning composite hydrogel has been optimized to create a bio-functional bioink, with improved printability, andin vitroculture stabilityviasecondary photo-induced cross-linking, making this composite a promising bioink for 3D bioprinting.
33662950	8	39	theme	property	1757:1764	arg1	result					1728:1733	a result	1726:1733	a result of the shear thinning property of the material	1726:1780	Shear stresses were relatively low while printing (1 kPa) as a result of the shear thinning property of the material, which supported cell viability during extrusion.
33662950	9	40	theme	concentrated	1962:1973	arg1	hydrogels					1975:1983	more concentrated hydrogels	1957:1983	more concentrated hydrogels when compared to cells cultured in bulk hydrogels, presumably due to improved nutrient/oxygen diffusion and cell migration	1957:2106	Finally, printed hydrogels retained high cell viability for lower gel concentrations, and showed improved cell viability for more concentrated hydrogels when compared to cells cultured in bulk hydrogels, presumably due to improved nutrient/oxygen diffusion and cell migration.
33662950	5	41	theme	high	1182:1185	arg1	concentrations					1191:1204	high gel concentrations	1182:1204	high gel concentrations (5+5 % XGMA-GelMA)	1182:1223	This was confirmed in printing studies, with measured normalized strand widths of 1.2 obtained for high gel concentrations (5+5 % XGMA-GelMA).
33662950	5	41	theme	high	1182:1185	arg1	XGMA-GelMA					1213:1222	5+5 % XGMA-GelMA	1207:1222	5+5 % XGMA-GelMA	1207:1222	This was confirmed in printing studies, with measured normalized strand widths of 1.2 obtained for high gel concentrations (5+5 % XGMA-GelMA).
33662950	5	42	theme	strand	1148:1153	arg1	widths					1155:1160	measured normalized strand widths	1128:1160	measured normalized strand widths of 1.2 obtained for high gel concentrations (5+5 % XGMA-GelMA)	1128:1223	This was confirmed in printing studies, with measured normalized strand widths of 1.2 obtained for high gel concentrations (5+5 % XGMA-GelMA).
33662950	9	43	theme	nutrient/oxygen	2063:2077	arg1	diffusion					2079:2087	improved nutrient/oxygen diffusion	2054:2087	improved nutrient/oxygen diffusion	2054:2087	Finally, printed hydrogels retained high cell viability for lower gel concentrations, and showed improved cell viability for more concentrated hydrogels when compared to cells cultured in bulk hydrogels, presumably due to improved nutrient/oxygen diffusion and cell migration.
33662950	0	44	theme	thinning	46:53	arg1	bio-functionality					17:33	bio-functionality	17:33	bio-functionality	17:33	Printability and bio-functionality of a shear thinning methacrylated xanthan-gelatin composite bioink.
33662950	0	44	theme	thinning	46:53	arg1	Printability					0:11	Printability	0:11	Printability	0:11	Printability and bio-functionality of a shear thinning methacrylated xanthan-gelatin composite bioink.
33662950	1	45	theme	cell	328:331	arg1	environment					333:343	the 3D cell environment	321:343	the 3D cell environment in tissues	321:354	3D bioprinting is a recent technique that can create complex cell seeded scaffolds and therefore holds great promise to revolutionize the biomedical sector by combining materials and structures that more closely mimic the 3D cell environment in tissues.
33662950	10	46	theme	vitroculture	2288:2299	arg1	cross-linking					2337:2349	andin vitroculture stabilityviasecondary photo-induced cross-linking	2282:2349	andin vitroculture stabilityviasecondary photo-induced cross-linking	2282:2349	In conclusion, stability and formulation of a XGMA-GelMA shear thinning composite hydrogel has been optimized to create a bio-functional bioink, with improved printability, andin vitroculture stabilityviasecondary photo-induced cross-linking, making this composite a promising bioink for 3D bioprinting.
33662950	9	47	theme	bulk	2020:2023	arg1	hydrogels					2025:2033	bulk hydrogels	2020:2033	bulk hydrogels	2020:2033	Finally, printed hydrogels retained high cell viability for lower gel concentrations, and showed improved cell viability for more concentrated hydrogels when compared to cells cultured in bulk hydrogels, presumably due to improved nutrient/oxygen diffusion and cell migration.
33662950	2	48	dep	hydrogels	410:418	arg1	present					463:469	present	463:469	present issues of printability, stability, or poor cell-material interactions	463:539	The most commonly used biomaterials for printing are hydrogels, however, many of the hydrogels used still present issues of printability, stability, or poor cell-material interactions.
33662950	4	49	theme	accurate	1049:1056	arg1	fidelity					1058:1065	accurate fidelity	1049:1065	accurate fidelity	1049:1065	These biomaterials have reduced viscosity under high shear and recover their viscosity rapidly after the shear is removed, retaining their shape, which translates to easier extrusion whilst maintaining accurate fidelity after printing.
33662950	6	50	theme	method	1291:1296	arg1	introduction					1243:1254	the introduction	1239:1254	the introduction of a secondary photo-cross-linking method	1239:1296	Furthermore, the introduction of a secondary photo-cross-linking method allowed tuning of the mechanical properties of the hydrogel with stiffness between 15 and 30 kPa, as well as improving the stability of the hydrogel with retention of 75 % of its mass after 90 d.
33662950	3	51	dep	methacrylated	656:668	arg1	XGMA					671:674	XGMA	671:674	XGMA	671:674	We propose that bioinks with intrinsic self-assembling and shear thinning properties, such as xanthan gum, can be methacrylated (XGMA) and combined with a bio-functional material such as gelatin methacryloyl (GelMa) to create a stable, cell-interactive bioink with improved properties for 3D bioprinting.
33662950	6	52	theme	secondary	1261:1269	arg1	method					1291:1296	a secondary photo-cross-linking method	1259:1296	a secondary photo-cross-linking method	1259:1296	Furthermore, the introduction of a secondary photo-cross-linking method allowed tuning of the mechanical properties of the hydrogel with stiffness between 15 and 30 kPa, as well as improving the stability of the hydrogel with retention of 75 % of its mass after 90 d.
33662950	6	53	theme	hydrogel	1438:1445	arg1	stability					1421:1429	the stability	1417:1429	the stability of the hydrogel	1417:1445	Furthermore, the introduction of a secondary photo-cross-linking method allowed tuning of the mechanical properties of the hydrogel with stiffness between 15 and 30 kPa, as well as improving the stability of the hydrogel with retention of 75 % of its mass after 90 d.
33662950	5	54	theme	5+5 	1207:1210	arg1	concentrations					1191:1204	high gel concentrations	1182:1204	high gel concentrations (5+5 % XGMA-GelMA)	1182:1223	This was confirmed in printing studies, with measured normalized strand widths of 1.2 obtained for high gel concentrations (5+5 % XGMA-GelMA).
33662950	5	54	theme	5+5 	1207:1210	arg1	XGMA-GelMA					1213:1222	5+5 % XGMA-GelMA	1207:1222	5+5 % XGMA-GelMA	1207:1222	This was confirmed in printing studies, with measured normalized strand widths of 1.2 obtained for high gel concentrations (5+5 % XGMA-GelMA).
33662950	4	55	theme	reduced	871:877	arg1	viscosity					879:887	reduced viscosity	871:887	reduced viscosity	871:887	These biomaterials have reduced viscosity under high shear and recover their viscosity rapidly after the shear is removed, retaining their shape, which translates to easier extrusion whilst maintaining accurate fidelity after printing.
33662950	9	56	theme	improved	2054:2061	arg1	diffusion					2079:2087	improved nutrient/oxygen diffusion	2054:2087	improved nutrient/oxygen diffusion	2054:2087	Finally, printed hydrogels retained high cell viability for lower gel concentrations, and showed improved cell viability for more concentrated hydrogels when compared to cells cultured in bulk hydrogels, presumably due to improved nutrient/oxygen diffusion and cell migration.
33662950	10	57	theme	stabilityviasecondary	2301:2321	arg1	cross-linking					2337:2349	andin vitroculture stabilityviasecondary photo-induced cross-linking	2282:2349	andin vitroculture stabilityviasecondary photo-induced cross-linking	2282:2349	In conclusion, stability and formulation of a XGMA-GelMA shear thinning composite hydrogel has been optimized to create a bio-functional bioink, with improved printability, andin vitroculture stabilityviasecondary photo-induced cross-linking, making this composite a promising bioink for 3D bioprinting.
33662950	3	58	theme	intrinsic	571:579	arg1	gum					644:646	xanthan gum	636:646	xanthan gum	636:646	We propose that bioinks with intrinsic self-assembling and shear thinning properties, such as xanthan gum, can be methacrylated (XGMA) and combined with a bio-functional material such as gelatin methacryloyl (GelMa) to create a stable, cell-interactive bioink with improved properties for 3D bioprinting.
33662950	3	58	theme	intrinsic	571:579	arg1	properties					616:625	intrinsic self-assembling and shear thinning properties	571:625	intrinsic self-assembling and shear thinning properties	571:625	We propose that bioinks with intrinsic self-assembling and shear thinning properties, such as xanthan gum, can be methacrylated (XGMA) and combined with a bio-functional material such as gelatin methacryloyl (GelMa) to create a stable, cell-interactive bioink with improved properties for 3D bioprinting.
33662950	9	59	theme	cell	2093:2096	arg1	migration					2098:2106	cell migration	2093:2106	cell migration	2093:2106	Finally, printed hydrogels retained high cell viability for lower gel concentrations, and showed improved cell viability for more concentrated hydrogels when compared to cells cultured in bulk hydrogels, presumably due to improved nutrient/oxygen diffusion and cell migration.
33662950	1	60	theme	great	206:210	arg1	promise					212:218	great promise	206:218	great promise to revolutionize the biomedical sector by combining materials and structures that more closely mimic the 3D cell environment in tissues	206:354	3D bioprinting is a recent technique that can create complex cell seeded scaffolds and therefore holds great promise to revolutionize the biomedical sector by combining materials and structures that more closely mimic the 3D cell environment in tissues.
33662950	6	61	theme	properties	1331:1340	arg1	tuning					1306:1311	tuning	1306:1311	tuning of the mechanical properties of the hydrogel with stiffness	1306:1371	Furthermore, the introduction of a secondary photo-cross-linking method allowed tuning of the mechanical properties of the hydrogel with stiffness between 15 and 30 kPa, as well as improving the stability of the hydrogel with retention of 75 % of its mass after 90 d.
33662950	3	62	theme	thinning	607:614	arg1	gum					644:646	xanthan gum	636:646	xanthan gum	636:646	We propose that bioinks with intrinsic self-assembling and shear thinning properties, such as xanthan gum, can be methacrylated (XGMA) and combined with a bio-functional material such as gelatin methacryloyl (GelMa) to create a stable, cell-interactive bioink with improved properties for 3D bioprinting.
33662950	3	62	theme	thinning	607:614	arg1	properties					616:625	intrinsic self-assembling and shear thinning properties	571:625	intrinsic self-assembling and shear thinning properties	571:625	We propose that bioinks with intrinsic self-assembling and shear thinning properties, such as xanthan gum, can be methacrylated (XGMA) and combined with a bio-functional material such as gelatin methacryloyl (GelMa) to create a stable, cell-interactive bioink with improved properties for 3D bioprinting.
33662950	8	63	theme	Shear	1665:1669	arg1	stresses					1671:1678	Shear stresses	1665:1678	Shear stresses	1665:1678	Shear stresses were relatively low while printing (1 kPa) as a result of the shear thinning property of the material, which supported cell viability during extrusion.
33662950	9	64	theme	high	1868:1871	arg1	viability					1878:1886	high cell viability	1868:1886	high cell viability	1868:1886	Finally, printed hydrogels retained high cell viability for lower gel concentrations, and showed improved cell viability for more concentrated hydrogels when compared to cells cultured in bulk hydrogels, presumably due to improved nutrient/oxygen diffusion and cell migration.
33662950	10	65	theme	a	2374:2374	arg1	bioink					2386:2391	this composite a promising bioink	2359:2391	this composite a promising bioink	2359:2391	In conclusion, stability and formulation of a XGMA-GelMA shear thinning composite hydrogel has been optimized to create a bio-functional bioink, with improved printability, andin vitroculture stabilityviasecondary photo-induced cross-linking, making this composite a promising bioink for 3D bioprinting.
33662950	1	66	theme	recent	123:128	arg1	bioprinting					106:116	3D bioprinting	103:116	3D bioprinting	103:116	3D bioprinting is a recent technique that can create complex cell seeded scaffolds and therefore holds great promise to revolutionize the biomedical sector by combining materials and structures that more closely mimic the 3D cell environment in tissues.
33662950	1	66	theme	recent	123:128	arg1	technique					130:138	a recent technique	121:138	a recent technique that can create complex cell seeded scaffolds and therefore holds great promise to revolutionize the biomedical sector by combining materials and structures that more closely mimic the 3D cell environment in tissues	121:354	3D bioprinting is a recent technique that can create complex cell seeded scaffolds and therefore holds great promise to revolutionize the biomedical sector by combining materials and structures that more closely mimic the 3D cell environment in tissues.
33662950	7	67	theme	%	1560:1560	arg1	viability					1567:1575	cell viability	1562:1575	cell viability	1562:1575	The hydrogel was shown to be biocompatible and bio-active with 97 % cell viability, and cell spreading after 7 d of culture for low gel concentrations (3+3 % XGMA-GelMA).
33662950	10	68	theme	andin	2282:2286	arg1	cross-linking					2337:2349	andin vitroculture stabilityviasecondary photo-induced cross-linking	2282:2349	andin vitroculture stabilityviasecondary photo-induced cross-linking	2282:2349	In conclusion, stability and formulation of a XGMA-GelMA shear thinning composite hydrogel has been optimized to create a bio-functional bioink, with improved printability, andin vitroculture stabilityviasecondary photo-induced cross-linking, making this composite a promising bioink for 3D bioprinting.
33662950	7	69	with	biocompatible	1523:1535	arg1	viability					1567:1575	cell viability	1562:1575	cell viability	1562:1575	The hydrogel was shown to be biocompatible and bio-active with 97 % cell viability, and cell spreading after 7 d of culture for low gel concentrations (3+3 % XGMA-GelMA).
33662950	7	69	with	biocompatible	1523:1535	arg1	spreading					1587:1595	cell spreading	1582:1595	cell spreading	1582:1595	The hydrogel was shown to be biocompatible and bio-active with 97 % cell viability, and cell spreading after 7 d of culture for low gel concentrations (3+3 % XGMA-GelMA).
33662950	9	70	theme	lower	1892:1896	arg1	concentrations					1902:1915	lower gel concentrations	1892:1915	lower gel concentrations	1892:1915	Finally, printed hydrogels retained high cell viability for lower gel concentrations, and showed improved cell viability for more concentrated hydrogels when compared to cells cultured in bulk hydrogels, presumably due to improved nutrient/oxygen diffusion and cell migration.
33662950	0	71	theme	composite	85:93	arg1	bioink					95:100	xanthan-gelatin composite bioink	69:100	xanthan-gelatin composite bioink	69:100	Printability and bio-functionality of a shear thinning methacrylated xanthan-gelatin composite bioink.
33662950	7	72	theme	3+3	1646:1648	arg1	XGMA-GelMA					1652:1661	3+3 % XGMA-GelMA	1646:1661	3+3 % XGMA-GelMA	1646:1661	The hydrogel was shown to be biocompatible and bio-active with 97 % cell viability, and cell spreading after 7 d of culture for low gel concentrations (3+3 % XGMA-GelMA).
33662950	7	72	theme	3+3	1646:1648	arg1	concentrations					1630:1643	low gel concentrations	1622:1643	low gel concentrations (3+3 % XGMA-GelMA)	1622:1662	The hydrogel was shown to be biocompatible and bio-active with 97 % cell viability, and cell spreading after 7 d of culture for low gel concentrations (3+3 % XGMA-GelMA).
33662950	2	73	theme	stability	495:503	arg1	issues					471:476	issues	471:476	issues of printability, stability, or poor cell-material interactions	471:539	The most commonly used biomaterials for printing are hydrogels, however, many of the hydrogels used still present issues of printability, stability, or poor cell-material interactions.
33662950	10	74	theme	composite	2364:2372	arg1	bioink					2386:2391	this composite a promising bioink	2359:2391	this composite a promising bioink	2359:2391	In conclusion, stability and formulation of a XGMA-GelMA shear thinning composite hydrogel has been optimized to create a bio-functional bioink, with improved printability, andin vitroculture stabilityviasecondary photo-induced cross-linking, making this composite a promising bioink for 3D bioprinting.
33662950	10	75	theme	hydrogel	2191:2198	arg1	formulation					2138:2148	formulation	2138:2148	formulation	2138:2148	In conclusion, stability and formulation of a XGMA-GelMA shear thinning composite hydrogel has been optimized to create a bio-functional bioink, with improved printability, andin vitroculture stabilityviasecondary photo-induced cross-linking, making this composite a promising bioink for 3D bioprinting.
33662950	10	75	theme	hydrogel	2191:2198	arg1	stability					2124:2132	stability	2124:2132	stability	2124:2132	In conclusion, stability and formulation of a XGMA-GelMA shear thinning composite hydrogel has been optimized to create a bio-functional bioink, with improved printability, andin vitroculture stabilityviasecondary photo-induced cross-linking, making this composite a promising bioink for 3D bioprinting.
33662950	3	76	with	bioink	795:800	arg1	properties					816:825	improved properties	807:825	improved properties for 3D bioprinting	807:844	We propose that bioinks with intrinsic self-assembling and shear thinning properties, such as xanthan gum, can be methacrylated (XGMA) and combined with a bio-functional material such as gelatin methacryloyl (GelMa) to create a stable, cell-interactive bioink with improved properties for 3D bioprinting.
33662950	2	77	theme	printability	481:492	arg1	issues					471:476	issues	471:476	issues of printability, stability, or poor cell-material interactions	471:539	The most commonly used biomaterials for printing are hydrogels, however, many of the hydrogels used still present issues of printability, stability, or poor cell-material interactions.
33662950	5	78	theme	normalized	1137:1146	arg1	widths					1155:1160	measured normalized strand widths	1128:1160	measured normalized strand widths of 1.2 obtained for high gel concentrations (5+5 % XGMA-GelMA)	1128:1223	This was confirmed in printing studies, with measured normalized strand widths of 1.2 obtained for high gel concentrations (5+5 % XGMA-GelMA).
33662950	4	79	contain	have	866:869	arg2	viscosity					879:887	reduced viscosity	871:887	reduced viscosity	871:887	These biomaterials have reduced viscosity under high shear and recover their viscosity rapidly after the shear is removed, retaining their shape, which translates to easier extrusion whilst maintaining accurate fidelity after printing.
33662950	4	79	contain	have	866:869	arg1	biomaterials					853:864	These biomaterials	847:864	These biomaterials	847:864	These biomaterials have reduced viscosity under high shear and recover their viscosity rapidly after the shear is removed, retaining their shape, which translates to easier extrusion whilst maintaining accurate fidelity after printing.
33662950	2	80	theme	cell-material	514:526	arg1	interactions					528:539	poor cell-material interactions	509:539	poor cell-material interactions	509:539	The most commonly used biomaterials for printing are hydrogels, however, many of the hydrogels used still present issues of printability, stability, or poor cell-material interactions.
33662950	9	81	theme	improved	1929:1936	arg1	viability					1943:1951	improved cell viability	1929:1951	improved cell viability	1929:1951	Finally, printed hydrogels retained high cell viability for lower gel concentrations, and showed improved cell viability for more concentrated hydrogels when compared to cells cultured in bulk hydrogels, presumably due to improved nutrient/oxygen diffusion and cell migration.
33662950	3	82	theme	improved	807:814	arg1	properties					816:825	improved properties	807:825	improved properties for 3D bioprinting	807:844	We propose that bioinks with intrinsic self-assembling and shear thinning properties, such as xanthan gum, can be methacrylated (XGMA) and combined with a bio-functional material such as gelatin methacryloyl (GelMa) to create a stable, cell-interactive bioink with improved properties for 3D bioprinting.
33662950	10	83	theme	XGMA-GelMA	2155:2164	arg1	hydrogel					2191:2198	a XGMA-GelMA shear thinning composite hydrogel	2153:2198	a XGMA-GelMA shear thinning composite hydrogel	2153:2198	In conclusion, stability and formulation of a XGMA-GelMA shear thinning composite hydrogel has been optimized to create a bio-functional bioink, with improved printability, andin vitroculture stabilityviasecondary photo-induced cross-linking, making this composite a promising bioink for 3D bioprinting.
33662950	10	84	theme	composite	2181:2189	arg1	hydrogel					2191:2198	a XGMA-GelMA shear thinning composite hydrogel	2153:2198	a XGMA-GelMA shear thinning composite hydrogel	2153:2198	In conclusion, stability and formulation of a XGMA-GelMA shear thinning composite hydrogel has been optimized to create a bio-functional bioink, with improved printability, andin vitroculture stabilityviasecondary photo-induced cross-linking, making this composite a promising bioink for 3D bioprinting.
33662950	3	85	with	bioinks	558:564	arg1	gum					644:646	xanthan gum	636:646	xanthan gum	636:646	We propose that bioinks with intrinsic self-assembling and shear thinning properties, such as xanthan gum, can be methacrylated (XGMA) and combined with a bio-functional material such as gelatin methacryloyl (GelMa) to create a stable, cell-interactive bioink with improved properties for 3D bioprinting.
33662950	3	85	with	bioinks	558:564	arg1	properties					616:625	intrinsic self-assembling and shear thinning properties	571:625	intrinsic self-assembling and shear thinning properties	571:625	We propose that bioinks with intrinsic self-assembling and shear thinning properties, such as xanthan gum, can be methacrylated (XGMA) and combined with a bio-functional material such as gelatin methacryloyl (GelMa) to create a stable, cell-interactive bioink with improved properties for 3D bioprinting.
33662950	1	86	theme	3D	103:104	arg1	bioprinting					106:116	3D bioprinting	103:116	3D bioprinting	103:116	3D bioprinting is a recent technique that can create complex cell seeded scaffolds and therefore holds great promise to revolutionize the biomedical sector by combining materials and structures that more closely mimic the 3D cell environment in tissues.
33662950	1	86	theme	3D	103:104	arg1	technique					130:138	a recent technique	121:138	a recent technique that can create complex cell seeded scaffolds and therefore holds great promise to revolutionize the biomedical sector by combining materials and structures that more closely mimic the 3D cell environment in tissues	121:354	3D bioprinting is a recent technique that can create complex cell seeded scaffolds and therefore holds great promise to revolutionize the biomedical sector by combining materials and structures that more closely mimic the 3D cell environment in tissues.
33662950	7	87	theme	low	1622:1624	arg1	XGMA-GelMA					1652:1661	3+3 % XGMA-GelMA	1646:1661	3+3 % XGMA-GelMA	1646:1661	The hydrogel was shown to be biocompatible and bio-active with 97 % cell viability, and cell spreading after 7 d of culture for low gel concentrations (3+3 % XGMA-GelMA).
33662950	7	87	theme	low	1622:1624	arg1	concentrations					1630:1643	low gel concentrations	1622:1643	low gel concentrations (3+3 % XGMA-GelMA)	1622:1662	The hydrogel was shown to be biocompatible and bio-active with 97 % cell viability, and cell spreading after 7 d of culture for low gel concentrations (3+3 % XGMA-GelMA).
33662950	2	88	theme	hydrogels	442:450	arg1	hydrogels					442:450	the hydrogels	438:450	the hydrogels used still	438:461	The most commonly used biomaterials for printing are hydrogels, however, many of the hydrogels used still present issues of printability, stability, or poor cell-material interactions.
33662950	2	88	theme	hydrogels	442:450	arg1	many					430:433	many	430:433	many	430:433	The most commonly used biomaterials for printing are hydrogels, however, many of the hydrogels used still present issues of printability, stability, or poor cell-material interactions.
33662950	10	89	theme	shear	2166:2170	arg1	hydrogel					2191:2198	a XGMA-GelMA shear thinning composite hydrogel	2153:2198	a XGMA-GelMA shear thinning composite hydrogel	2153:2198	In conclusion, stability and formulation of a XGMA-GelMA shear thinning composite hydrogel has been optimized to create a bio-functional bioink, with improved printability, andin vitroculture stabilityviasecondary photo-induced cross-linking, making this composite a promising bioink for 3D bioprinting.
33662950	1	90	dep	seeded	169:174	arg1	cell					164:167	complex cell	156:167	complex cell	156:167	3D bioprinting is a recent technique that can create complex cell seeded scaffolds and therefore holds great promise to revolutionize the biomedical sector by combining materials and structures that more closely mimic the 3D cell environment in tissues.
33662950	8	91	theme	thinning	1748:1755	arg1	property					1757:1764	the shear thinning property	1738:1764	the shear thinning property of the material	1738:1780	Shear stresses were relatively low while printing (1 kPa) as a result of the shear thinning property of the material, which supported cell viability during extrusion.
33662950	7	92	theme	culture	1610:1616	arg1	viability					1567:1575	cell viability	1562:1575	cell viability	1562:1575	The hydrogel was shown to be biocompatible and bio-active with 97 % cell viability, and cell spreading after 7 d of culture for low gel concentrations (3+3 % XGMA-GelMA).
33662950	7	92	theme	culture	1610:1616	arg1	spreading					1587:1595	cell spreading	1582:1595	cell spreading	1582:1595	The hydrogel was shown to be biocompatible and bio-active with 97 % cell viability, and cell spreading after 7 d of culture for low gel concentrations (3+3 % XGMA-GelMA).
33662950	10	93	theme	promising	2376:2384	arg1	bioink					2386:2391	this composite a promising bioink	2359:2391	this composite a promising bioink	2359:2391	In conclusion, stability and formulation of a XGMA-GelMA shear thinning composite hydrogel has been optimized to create a bio-functional bioink, with improved printability, andin vitroculture stabilityviasecondary photo-induced cross-linking, making this composite a promising bioink for 3D bioprinting.
33662950	6	94	theme	mass	1477:1480	arg1	%					1468:1468	75 %	1465:1468	75 % of its mass	1465:1480	Furthermore, the introduction of a secondary photo-cross-linking method allowed tuning of the mechanical properties of the hydrogel with stiffness between 15 and 30 kPa, as well as improving the stability of the hydrogel with retention of 75 % of its mass after 90 d.
33662950	6	94	theme	mass	1477:1480	arg1	mass					1477:1480	its mass	1473:1480	its mass	1473:1480	Furthermore, the introduction of a secondary photo-cross-linking method allowed tuning of the mechanical properties of the hydrogel with stiffness between 15 and 30 kPa, as well as improving the stability of the hydrogel with retention of 75 % of its mass after 90 d.
33662950	0	95	theme	shear	40:44	arg1	thinning					46:53	a shear thinning	38:53	a shear thinning	38:53	Printability and bio-functionality of a shear thinning methacrylated xanthan-gelatin composite bioink.
33662950	5	96	theme	gel	1187:1189	arg1	concentrations					1191:1204	high gel concentrations	1182:1204	high gel concentrations (5+5 % XGMA-GelMA)	1182:1223	This was confirmed in printing studies, with measured normalized strand widths of 1.2 obtained for high gel concentrations (5+5 % XGMA-GelMA).
33662950	5	96	theme	gel	1187:1189	arg1	XGMA-GelMA					1213:1222	5+5 % XGMA-GelMA	1207:1222	5+5 % XGMA-GelMA	1207:1222	This was confirmed in printing studies, with measured normalized strand widths of 1.2 obtained for high gel concentrations (5+5 % XGMA-GelMA).
33662950	1	97	theme	3D	325:326	arg1	environment					333:343	the 3D cell environment	321:343	the 3D cell environment in tissues	321:354	3D bioprinting is a recent technique that can create complex cell seeded scaffolds and therefore holds great promise to revolutionize the biomedical sector by combining materials and structures that more closely mimic the 3D cell environment in tissues.
33662950	5	98	theme	1.2	1165:1167	arg1	widths					1155:1160	measured normalized strand widths	1128:1160	measured normalized strand widths of 1.2 obtained for high gel concentrations (5+5 % XGMA-GelMA)	1128:1223	This was confirmed in printing studies, with measured normalized strand widths of 1.2 obtained for high gel concentrations (5+5 % XGMA-GelMA).
33662950	6	99	theme	%	1468:1468	arg1	retention					1452:1460	retention	1452:1460	retention of 75 % of its mass after 90 d	1452:1491	Furthermore, the introduction of a secondary photo-cross-linking method allowed tuning of the mechanical properties of the hydrogel with stiffness between 15 and 30 kPa, as well as improving the stability of the hydrogel with retention of 75 % of its mass after 90 d.
33662950	1	100	theme	biomedical	241:250	arg1	sector					252:257	the biomedical sector	237:257	the biomedical sector	237:257	3D bioprinting is a recent technique that can create complex cell seeded scaffolds and therefore holds great promise to revolutionize the biomedical sector by combining materials and structures that more closely mimic the 3D cell environment in tissues.
33662950	2	101	theme	used	375:378	arg1	hydrogels					410:418	hydrogels	410:418	hydrogels	410:418	The most commonly used biomaterials for printing are hydrogels, however, many of the hydrogels used still present issues of printability, stability, or poor cell-material interactions.
33662950	2	101	theme	used	375:378	arg1	biomaterials					380:391	The most commonly used biomaterials	357:391	The most commonly used biomaterials for printing	357:404	The most commonly used biomaterials for printing are hydrogels, however, many of the hydrogels used still present issues of printability, stability, or poor cell-material interactions.
32785468	1	0	theme	vulgaris	289:296	arg1	L					298:298	Phaseolus vulgaris L	279:298	Phaseolus vulgaris L	279:298	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	4	1	theme	restricted-swelling	828:846	arg1	starch					848:853	highly restricted-swelling starch	821:853	highly restricted-swelling starch	821:853	The swelling power of the legume starch isolates put them in the category of highly restricted-swelling starch.
32785468	9	2	theme	final	1445:1449	arg1	FV					1462:1463	FV	1462:1463	FV	1462:1463	The factors which influence the pasting characteristics resulting to decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch are attributed to the interaction of starch with the protein, fat, etc. which depended to their variety.
32785468	9	2	theme	final	1445:1449	arg1	viscosity					1451:1459	final viscosity	1445:1459	final viscosity (FV)	1445:1464	The factors which influence the pasting characteristics resulting to decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch are attributed to the interaction of starch with the protein, fat, etc. which depended to their variety.
32785468	1	3	theme	Vigna	217:221	arg1	V					235:235	Vigna subterrenea V	217:235	Vigna subterrenea V	217:235	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	5	4	with	noodles	941:947	arg1	cereals					975:981	cereals	975:981	cereals	975:981	This characteristic is desirable for the manufacture of value-added products such as noodles and composite blends with cereals.
32785468	1	5	theme	L	298:298	arg1	L					189:189	Vigna unguiculata L	171:189	Vigna unguiculata L. (Cowpea)	171:199	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	5	theme	L	298:298	arg1	varieties					266:274	six varieties	262:274	six varieties of Phaseolus vulgaris L. (Common bean)	262:313	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	5	theme	L	298:298	arg1	variety					206:212	one variety	202:212	one variety of Vigna subterrenea V. (Bambara groundnut)	202:256	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	5	theme	L	298:298	arg1	bean					309:312	Common bean	302:312	Common bean	302:312	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	5	theme	L	298:298	arg1	groundnut					247:255	Bambara groundnut	239:255	Bambara groundnut	239:255	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	5	theme	L	298:298	arg1	Cowpea					193:198	Cowpea	193:198	Cowpea	193:198	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	5	theme	L	298:298	arg1	L					298:298	Phaseolus vulgaris L	279:298	Phaseolus vulgaris L	279:298	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	5	theme	L	298:298	arg1	V					235:235	Vigna subterrenea V	217:235	Vigna subterrenea V	217:235	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	6	theme	variety	160:166	arg1	cultivars					136:144	eight legumes cultivars instar	122:151	eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean)	122:313	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	7	theme	subterrenea	223:233	arg1	V					235:235	Vigna subterrenea V	217:235	Vigna subterrenea V	217:235	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	2	8	theme	beans	616:620	arg1	functionality					599:611	the functionality	595:611	the functionality of beans and their starch grown in the region	595:657	The objectives of the study were to investigate the starch properties and processing characteristics of different bean varieties, and to establish the basic foundation of improving the functionality of beans and their starch grown in the region.
32785468	2	9	theme	varieties	533:541	arg1	properties					473:482	starch properties	466:482	starch properties	466:482	The objectives of the study were to investigate the starch properties and processing characteristics of different bean varieties, and to establish the basic foundation of improving the functionality of beans and their starch grown in the region.
32785468	2	9	theme	varieties	533:541	arg1	characteristics					499:513	processing characteristics	488:513	processing characteristics	488:513	The objectives of the study were to investigate the starch properties and processing characteristics of different bean varieties, and to establish the basic foundation of improving the functionality of beans and their starch grown in the region.
32785468	1	10	theme	physicochemical	359:373	arg1	properties					387:396	their physicochemical and pasting properties	353:396	their physicochemical and pasting properties	353:396	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	11	theme	V	235:235	arg1	L					189:189	Vigna unguiculata L	171:189	Vigna unguiculata L. (Cowpea)	171:199	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	11	theme	V	235:235	arg1	varieties					266:274	six varieties	262:274	six varieties of Phaseolus vulgaris L. (Common bean)	262:313	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	11	theme	V	235:235	arg1	variety					206:212	one variety	202:212	one variety of Vigna subterrenea V. (Bambara groundnut)	202:256	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	11	theme	V	235:235	arg1	bean					309:312	Common bean	302:312	Common bean	302:312	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	11	theme	V	235:235	arg1	groundnut					247:255	Bambara groundnut	239:255	Bambara groundnut	239:255	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	11	theme	V	235:235	arg1	Cowpea					193:198	Cowpea	193:198	Cowpea	193:198	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	11	theme	V	235:235	arg1	L					298:298	Phaseolus vulgaris L	279:298	Phaseolus vulgaris L	279:298	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	11	theme	V	235:235	arg1	V					235:235	Vigna subterrenea V	217:235	Vigna subterrenea V	217:235	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	0	12	from	varieties	62:70	arg1	potential					14:22	Technological potential	0:22	Technological potential of under-utilized starches from eight varieties of legumes	0:81	Technological potential of under-utilized starches from eight varieties of legumes grown in Cameroon.
32785468	2	13	theme	bean	528:531	arg1	varieties					533:541	different bean varieties	518:541	different bean varieties	518:541	The objectives of the study were to investigate the starch properties and processing characteristics of different bean varieties, and to establish the basic foundation of improving the functionality of beans and their starch grown in the region.
32785468	7	14	theme	Bambara	1186:1192	arg1	groundnut					1194:1202	Bambara groundnut	1186:1202	Bambara groundnut	1186:1202	The high breakdown viscosity (BV) was founded for Cowpea and Bambara groundnut and confirmed their low.
32785468	5	15	theme	value-added	912:922	arg1	products					924:931	value-added products	912:931	value-added products such as noodles and composite blends with cereals	912:981	This characteristic is desirable for the manufacture of value-added products such as noodles and composite blends with cereals.
32785468	5	15	theme	value-added	912:922	arg1	noodles					941:947	noodles	941:947	noodles	941:947	This characteristic is desirable for the manufacture of value-added products such as noodles and composite blends with cereals.
32785468	5	15	theme	value-added	912:922	arg1	blends					963:968	composite blends	953:968	composite blends	953:968	This characteristic is desirable for the manufacture of value-added products such as noodles and composite blends with cereals.
32785468	5	16	with	blends	963:968	arg1	cereals					975:981	cereals	975:981	cereals	975:981	This characteristic is desirable for the manufacture of value-added products such as noodles and composite blends with cereals.
32785468	1	17	theme	Common	302:307	arg1	L					298:298	Phaseolus vulgaris L	279:298	Phaseolus vulgaris L	279:298	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	17	theme	Common	302:307	arg1	varieties					266:274	six varieties	262:274	six varieties of Phaseolus vulgaris L. (Common bean)	262:313	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	17	theme	Common	302:307	arg1	V					235:235	Vigna subterrenea V	217:235	Vigna subterrenea V	217:235	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	17	theme	Common	302:307	arg1	bean					309:312	Common bean	302:312	Common bean	302:312	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	2	18	theme	basic	565:569	arg1	foundation					571:580	the basic foundation	561:580	the basic foundation of improving the functionality of beans and their starch grown in the region	561:657	The objectives of the study were to investigate the starch properties and processing characteristics of different bean varieties, and to establish the basic foundation of improving the functionality of beans and their starch grown in the region.
32785468	1	19	theme	pasting	379:385	arg1	properties					387:396	their physicochemical and pasting properties	353:396	their physicochemical and pasting properties	353:396	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	5	20	theme	products	924:931	arg1	manufacture					897:907	the manufacture	893:907	the manufacture of value-added products such as noodles and composite blends with cereals	893:981	This characteristic is desirable for the manufacture of value-added products such as noodles and composite blends with cereals.
32785468	9	21	theme	peak	1398:1401	arg1	PV					1414:1415	PV	1414:1415	PV	1414:1415	The factors which influence the pasting characteristics resulting to decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch are attributed to the interaction of starch with the protein, fat, etc. which depended to their variety.
32785468	9	21	theme	peak	1398:1401	arg1	viscosity					1403:1411	peak viscosity	1398:1411	peak viscosity (PV)	1398:1416	The factors which influence the pasting characteristics resulting to decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch are attributed to the interaction of starch with the protein, fat, etc. which depended to their variety.
32785468	6	22	theme	rapid	1031:1035	arg1	analyzer					1043:1050	a rapid visco analyzer	1029:1050	a rapid visco analyzer	1029:1050	The pasting properties were determined using a rapid visco analyzer, and various legumes bean starches exhibited different pasting profiles.
32785468	2	23	theme	study	436:440	arg1	objectives					418:427	The objectives	414:427	The objectives of the study	414:440	The objectives of the study were to investigate the starch properties and processing characteristics of different bean varieties, and to establish the basic foundation of improving the functionality of beans and their starch grown in the region.
32785468	4	24	theme	legume	770:775	arg1	isolates					784:791	the legume starch isolates	766:791	the legume starch isolates	766:791	The swelling power of the legume starch isolates put them in the category of highly restricted-swelling starch.
32785468	7	25	theme	breakdown	1134:1142	arg1	BV					1155:1156	BV	1155:1156	BV	1155:1156	The high breakdown viscosity (BV) was founded for Cowpea and Bambara groundnut and confirmed their low.
32785468	7	25	theme	breakdown	1134:1142	arg1	viscosity					1144:1152	The high breakdown viscosity	1125:1152	The high breakdown viscosity (BV)	1125:1157	The high breakdown viscosity (BV) was founded for Cowpea and Bambara groundnut and confirmed their low.
32785468	1	26	theme	Vigna	171:175	arg1	L					189:189	Vigna unguiculata L	171:189	Vigna unguiculata L. (Cowpea)	171:199	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	26	theme	Vigna	171:175	arg1	Cowpea					193:198	Cowpea	193:198	Cowpea	193:198	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	7	27	theme	high	1129:1132	arg1	BV					1155:1156	BV	1155:1156	BV	1155:1156	The high breakdown viscosity (BV) was founded for Cowpea and Bambara groundnut and confirmed their low.
32785468	7	27	theme	high	1129:1132	arg1	viscosity					1144:1152	The high breakdown viscosity	1125:1152	The high breakdown viscosity (BV)	1125:1157	The high breakdown viscosity (BV) was founded for Cowpea and Bambara groundnut and confirmed their low.
32785468	9	28	with	interaction	1498:1508	arg1	etc.					1543:1546	etc.	1543:1546	etc.	1543:1546	The factors which influence the pasting characteristics resulting to decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch are attributed to the interaction of starch with the protein, fat, etc. which depended to their variety.
32785468	9	28	with	interaction	1498:1508	arg1	fat					1538:1540	fat	1538:1540	fat	1538:1540	The factors which influence the pasting characteristics resulting to decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch are attributed to the interaction of starch with the protein, fat, etc. which depended to their variety.
32785468	9	28	with	interaction	1498:1508	arg1	protein					1529:1535	the protein	1525:1535	the protein	1525:1535	The factors which influence the pasting characteristics resulting to decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch are attributed to the interaction of starch with the protein, fat, etc. which depended to their variety.
32785468	1	29	from	cultivars	136:144	arg1	samples					109:115	Starch samples	102:115	Starch samples	102:115	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	9	30	theme	starch	1469:1474	arg1	decrease					1386:1393	decrease	1386:1393	decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch	1386:1474	The factors which influence the pasting characteristics resulting to decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch are attributed to the interaction of starch with the protein, fat, etc. which depended to their variety.
32785468	1	31	theme	unguiculata	177:187	arg1	L					189:189	Vigna unguiculata L	171:189	Vigna unguiculata L. (Cowpea)	171:199	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	31	theme	unguiculata	177:187	arg1	Cowpea					193:198	Cowpea	193:198	Cowpea	193:198	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	0	32	theme	Technological	0:12	arg1	potential					14:22	Technological potential	0:22	Technological potential of under-utilized starches from eight varieties of legumes	0:81	Technological potential of under-utilized starches from eight varieties of legumes grown in Cameroon.
32785468	3	33	theme	significant	680:690	arg1	differences					692:702	significant differences	680:702	significant differences amongst the properties of the starches	680:741	The result revealed significant differences amongst the properties of the starches.
32785468	4	34	theme	isolates	784:791	arg1	power					757:761	The swelling power	744:761	The swelling power of the legume starch isolates	744:791	The swelling power of the legume starch isolates put them in the category of highly restricted-swelling starch.
32785468	4	35	theme	starch	777:782	arg1	isolates					784:791	the legume starch isolates	766:791	the legume starch isolates	766:791	The swelling power of the legume starch isolates put them in the category of highly restricted-swelling starch.
32785468	1	36	theme	L	189:189	arg1	L					189:189	Vigna unguiculata L	171:189	Vigna unguiculata L. (Cowpea)	171:199	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	36	theme	L	189:189	arg1	varieties					266:274	six varieties	262:274	six varieties of Phaseolus vulgaris L. (Common bean)	262:313	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	36	theme	L	189:189	arg1	variety					206:212	one variety	202:212	one variety of Vigna subterrenea V. (Bambara groundnut)	202:256	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	36	theme	L	189:189	arg1	bean					309:312	Common bean	302:312	Common bean	302:312	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	36	theme	L	189:189	arg1	variety					160:166	one variety	156:166	one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean)	156:313	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	36	theme	L	189:189	arg1	groundnut					247:255	Bambara groundnut	239:255	Bambara groundnut	239:255	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	36	theme	L	189:189	arg1	Cowpea					193:198	Cowpea	193:198	Cowpea	193:198	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	36	theme	L	189:189	arg1	L					298:298	Phaseolus vulgaris L	279:298	Phaseolus vulgaris L	279:298	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	36	theme	L	189:189	arg1	V					235:235	Vigna subterrenea V	217:235	Vigna subterrenea V	217:235	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	0	37	theme	under-utilized	27:40	arg1	starches					42:49	under-utilized starches	27:49	under-utilized starches	27:49	Technological potential of under-utilized starches from eight varieties of legumes grown in Cameroon.
32785468	1	38	theme	Bambara	239:245	arg1	groundnut					247:255	Bambara groundnut	239:255	Bambara groundnut	239:255	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	38	theme	Bambara	239:245	arg1	variety					206:212	one variety	202:212	one variety of Vigna subterrenea V. (Bambara groundnut)	202:256	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	38	theme	Bambara	239:245	arg1	V					235:235	Vigna subterrenea V	217:235	Vigna subterrenea V	217:235	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	38	theme	Bambara	239:245	arg1	L					298:298	Phaseolus vulgaris L	279:298	Phaseolus vulgaris L	279:298	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	39	theme	Starch	102:107	arg1	samples					109:115	Starch samples	102:115	Starch samples	102:115	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	6	40	theme	pasting	1107:1113	arg1	profiles					1115:1122	different pasting profiles	1097:1122	different pasting profiles	1097:1122	The pasting properties were determined using a rapid visco analyzer, and various legumes bean starches exhibited different pasting profiles.
32785468	8	41	theme	bean	1293:1296	arg1	studies					1308:1314	Common bean varieties studies	1286:1314	Common bean varieties studies	1286:1314	ability to resist heat and shear stress when compared to Common bean varieties studies.
32785468	6	42	theme	visco	1037:1041	arg1	analyzer					1043:1050	a rapid visco analyzer	1029:1050	a rapid visco analyzer	1029:1050	The pasting properties were determined using a rapid visco analyzer, and various legumes bean starches exhibited different pasting profiles.
32785468	8	43	theme	shear	1256:1260	arg1	stress					1262:1267	shear stress	1256:1267	shear stress	1256:1267	ability to resist heat and shear stress when compared to Common bean varieties studies.
32785468	8	44	theme	Common	1286:1291	arg1	studies					1308:1314	Common bean varieties studies	1286:1314	Common bean varieties studies	1286:1314	ability to resist heat and shear stress when compared to Common bean varieties studies.
32785468	2	45	theme	starch	466:471	arg1	properties					473:482	starch properties	466:482	starch properties	466:482	The objectives of the study were to investigate the starch properties and processing characteristics of different bean varieties, and to establish the basic foundation of improving the functionality of beans and their starch grown in the region.
32785468	6	46	theme	pasting	988:994	arg1	properties					996:1005	The pasting properties	984:1005	The pasting properties	984:1005	The pasting properties were determined using a rapid visco analyzer, and various legumes bean starches exhibited different pasting profiles.
32785468	0	47	theme	starches	42:49	arg1	potential					14:22	Technological potential	0:22	Technological potential of under-utilized starches from eight varieties of legumes	0:81	Technological potential of under-utilized starches from eight varieties of legumes grown in Cameroon.
32785468	4	48	theme	swelling	748:755	arg1	power					757:761	The swelling power	744:761	The swelling power of the legume starch isolates	744:791	The swelling power of the legume starch isolates put them in the category of highly restricted-swelling starch.
32785468	9	49	theme	pasting	1349:1355	arg1	characteristics					1357:1371	the pasting characteristics	1345:1371	the pasting characteristics resulting to decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch	1345:1474	The factors which influence the pasting characteristics resulting to decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch are attributed to the interaction of starch with the protein, fat, etc. which depended to their variety.
32785468	2	50	dep	properties	473:482	arg1	the					462:464	the	462:464	the	462:464	The objectives of the study were to investigate the starch properties and processing characteristics of different bean varieties, and to establish the basic foundation of improving the functionality of beans and their starch grown in the region.
32785468	2	51	theme	different	518:526	arg1	varieties					533:541	different bean varieties	518:541	different bean varieties	518:541	The objectives of the study were to investigate the starch properties and processing characteristics of different bean varieties, and to establish the basic foundation of improving the functionality of beans and their starch grown in the region.
32785468	8	52	theme	varieties	1298:1306	arg1	studies					1308:1314	Common bean varieties studies	1286:1314	Common bean varieties studies	1286:1314	ability to resist heat and shear stress when compared to Common bean varieties studies.
32785468	9	53	theme	trough	1419:1424	arg1	TV					1437:1438	TV	1437:1438	TV	1437:1438	The factors which influence the pasting characteristics resulting to decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch are attributed to the interaction of starch with the protein, fat, etc. which depended to their variety.
32785468	9	53	theme	trough	1419:1424	arg1	viscosity					1426:1434	trough viscosity	1419:1434	trough viscosity (TV)	1419:1439	The factors which influence the pasting characteristics resulting to decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch are attributed to the interaction of starch with the protein, fat, etc. which depended to their variety.
32785468	1	54	theme	varieties	266:274	arg1	L					189:189	Vigna unguiculata L	171:189	Vigna unguiculata L. (Cowpea)	171:199	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	54	theme	varieties	266:274	arg1	varieties					266:274	six varieties	262:274	six varieties of Phaseolus vulgaris L. (Common bean)	262:313	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	54	theme	varieties	266:274	arg1	variety					206:212	one variety	202:212	one variety of Vigna subterrenea V. (Bambara groundnut)	202:256	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	54	theme	varieties	266:274	arg1	bean					309:312	Common bean	302:312	Common bean	302:312	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	54	theme	varieties	266:274	arg1	variety					160:166	one variety	156:166	one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean)	156:313	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	54	theme	varieties	266:274	arg1	groundnut					247:255	Bambara groundnut	239:255	Bambara groundnut	239:255	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	54	theme	varieties	266:274	arg1	Cowpea					193:198	Cowpea	193:198	Cowpea	193:198	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	54	theme	varieties	266:274	arg1	L					298:298	Phaseolus vulgaris L	279:298	Phaseolus vulgaris L	279:298	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	54	theme	varieties	266:274	arg1	V					235:235	Vigna subterrenea V	217:235	Vigna subterrenea V	217:235	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	9	55	theme	starch	1513:1518	arg1	interaction					1498:1508	the interaction	1494:1508	the interaction of starch with the protein, fat, etc. which depended to their variety	1494:1578	The factors which influence the pasting characteristics resulting to decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch are attributed to the interaction of starch with the protein, fat, etc. which depended to their variety.
32785468	2	56	theme	starch	632:637	arg1	functionality					599:611	the functionality	595:611	the functionality of beans and their starch grown in the region	595:657	The objectives of the study were to investigate the starch properties and processing characteristics of different bean varieties, and to establish the basic foundation of improving the functionality of beans and their starch grown in the region.
32785468	6	57	theme	bean	1073:1076	arg1	starches					1078:1085	various legumes bean starches	1057:1085	various legumes bean starches	1057:1085	The pasting properties were determined using a rapid visco analyzer, and various legumes bean starches exhibited different pasting profiles.
32785468	4	58	theme	starch	848:853	arg1	category					809:816	the category	805:816	the category of highly restricted-swelling starch	805:853	The swelling power of the legume starch isolates put them in the category of highly restricted-swelling starch.
32785468	1	59	theme	legumes	128:134	arg1	cultivars					136:144	eight legumes cultivars instar	122:151	eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean)	122:313	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	6	60	theme	different	1097:1105	arg1	profiles					1115:1122	different pasting profiles	1097:1122	different pasting profiles	1097:1122	The pasting properties were determined using a rapid visco analyzer, and various legumes bean starches exhibited different pasting profiles.
32785468	2	61	theme	processing	488:497	arg1	characteristics					499:513	processing characteristics	488:513	processing characteristics	488:513	The objectives of the study were to investigate the starch properties and processing characteristics of different bean varieties, and to establish the basic foundation of improving the functionality of beans and their starch grown in the region.
32785468	6	62	theme	various	1057:1063	arg1	starches					1078:1085	various legumes bean starches	1057:1085	various legumes bean starches	1057:1085	The pasting properties were determined using a rapid visco analyzer, and various legumes bean starches exhibited different pasting profiles.
32785468	6	63	theme	legumes	1065:1071	arg1	starches					1078:1085	various legumes bean starches	1057:1085	various legumes bean starches	1057:1085	The pasting properties were determined using a rapid visco analyzer, and various legumes bean starches exhibited different pasting profiles.
32785468	0	64	theme	legumes	75:81	arg1	potential					14:22	Technological potential	0:22	Technological potential of under-utilized starches from eight varieties of legumes	0:81	Technological potential of under-utilized starches from eight varieties of legumes grown in Cameroon.
32785468	1	65	theme	instar	146:151	arg1	cultivars					136:144	eight legumes cultivars instar	122:151	eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean)	122:313	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	66	theme	variety	206:212	arg1	L					189:189	Vigna unguiculata L	171:189	Vigna unguiculata L. (Cowpea)	171:199	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	66	theme	variety	206:212	arg1	varieties					266:274	six varieties	262:274	six varieties of Phaseolus vulgaris L. (Common bean)	262:313	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	66	theme	variety	206:212	arg1	variety					206:212	one variety	202:212	one variety of Vigna subterrenea V. (Bambara groundnut)	202:256	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	66	theme	variety	206:212	arg1	bean					309:312	Common bean	302:312	Common bean	302:312	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	66	theme	variety	206:212	arg1	variety					160:166	one variety	156:166	one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean)	156:313	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	66	theme	variety	206:212	arg1	groundnut					247:255	Bambara groundnut	239:255	Bambara groundnut	239:255	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	66	theme	variety	206:212	arg1	Cowpea					193:198	Cowpea	193:198	Cowpea	193:198	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	66	theme	variety	206:212	arg1	L					298:298	Phaseolus vulgaris L	279:298	Phaseolus vulgaris L	279:298	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	66	theme	variety	206:212	arg1	V					235:235	Vigna subterrenea V	217:235	Vigna subterrenea V	217:235	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	1	67	theme	Phaseolus	279:287	arg1	vulgaris					289:296	Phaseolus vulgaris	279:296	Phaseolus vulgaris L	279:298	Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated.
32785468	5	68	theme	composite	953:961	arg1	blends					963:968	composite blends	953:968	composite blends	953:968	This characteristic is desirable for the manufacture of value-added products such as noodles and composite blends with cereals.
32785468	9	69	from	decrease	1386:1393	arg1	viscosity					1451:1459	final viscosity	1445:1459	final viscosity (FV)	1445:1464	The factors which influence the pasting characteristics resulting to decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch are attributed to the interaction of starch with the protein, fat, etc. which depended to their variety.
32785468	9	69	from	decrease	1386:1393	arg1	viscosity					1403:1411	peak viscosity	1398:1411	peak viscosity (PV)	1398:1416	The factors which influence the pasting characteristics resulting to decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch are attributed to the interaction of starch with the protein, fat, etc. which depended to their variety.
32785468	9	69	from	decrease	1386:1393	arg1	viscosity					1426:1434	trough viscosity	1419:1434	trough viscosity (TV)	1419:1439	The factors which influence the pasting characteristics resulting to decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch are attributed to the interaction of starch with the protein, fat, etc. which depended to their variety.
32785468	9	69	from	decrease	1386:1393	arg1	TV					1437:1438	TV	1437:1438	TV	1437:1438	The factors which influence the pasting characteristics resulting to decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch are attributed to the interaction of starch with the protein, fat, etc. which depended to their variety.
32785468	9	69	from	decrease	1386:1393	arg1	FV					1462:1463	FV	1462:1463	FV	1462:1463	The factors which influence the pasting characteristics resulting to decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch are attributed to the interaction of starch with the protein, fat, etc. which depended to their variety.
32785468	9	69	from	decrease	1386:1393	arg1	PV					1414:1415	PV	1414:1415	PV	1414:1415	The factors which influence the pasting characteristics resulting to decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch are attributed to the interaction of starch with the protein, fat, etc. which depended to their variety.
32785468	3	70	theme	starches	734:741	arg1	properties					716:725	the properties	712:725	the properties of the starches	712:741	The result revealed significant differences amongst the properties of the starches.
32084482	7	0	theme	bone	1363:1366	arg1	applications					1387:1398	bone tissue engineering applications	1363:1398	bone tissue engineering applications	1363:1398	Thus, the developed composite scaffold might be a suitable biomaterial for bone tissue engineering applications.
32084482	2	1	from	resuspension	501:512	arg1	matrix					546:551	gelatin matrix	538:551	gelatin matrix	538:551	In the present study, nano-bioglass incorporated osteoconductive composite scaffolds were fabricated through polyelectrolyte complexation/phase separation and resuspension of separated complex in gelatin matrix.
32084482	1	2	theme	composite	166:174	arg1	materials					176:184	various composite materials	158:184	various composite materials fabricated using natural or synthetic biopolymers incorporated with bioceramic	158:263	Over the past decade, various composite materials fabricated using natural or synthetic biopolymers incorporated with bioceramic have been widely investigated for the regeneration of segmental bone defect.
32084482	6	3	theme	obtained	1127:1134	arg1	analysis					1149:1156	The obtained histological analysis	1123:1156	The obtained histological analysis	1123:1156	The obtained histological analysis indicates strong potential of the composite scaffold for bone tissue regeneration, vascularization and reconstruction of defects.
32084482	6	4	theme	composite	1192:1200	arg1	scaffold					1202:1209	the composite scaffold	1188:1209	the composite scaffold	1188:1209	The obtained histological analysis indicates strong potential of the composite scaffold for bone tissue regeneration, vascularization and reconstruction of defects.
32084482	5	5	theme	tissue	1005:1010	arg1	regeneration					1012:1023	bone tissue regeneration	1000:1023	bone tissue regeneration potential through implantation in non-union segmental bone defect created in rabbit animal model	1000:1120	Also, the developed scaffold was evaluated for biocompatibility and bone tissue regeneration potential through implantation in non-union segmental bone defect created in rabbit animal model.
32084482	2	6	theme	osteoconductive	391:405	arg1	scaffolds					417:425	nano-bioglass incorporated osteoconductive composite scaffolds	364:425	nano-bioglass incorporated osteoconductive composite scaffolds	364:425	In the present study, nano-bioglass incorporated osteoconductive composite scaffolds were fabricated through polyelectrolyte complexation/phase separation and resuspension of separated complex in gelatin matrix.
32084482	0	7	theme	tissue	116:121	arg1	engineering					123:133	bone tissue engineering	111:133	bone tissue engineering	111:133	Generation of scaffold incorporated with nanobioglass encapsulated in chitosan/chondroitin sulfate complex for bone tissue engineering.
32084482	5	8	theme	bone	1000:1003	arg1	regeneration					1012:1023	bone tissue regeneration	1000:1023	bone tissue regeneration potential through implantation in non-union segmental bone defect created in rabbit animal model	1000:1120	Also, the developed scaffold was evaluated for biocompatibility and bone tissue regeneration potential through implantation in non-union segmental bone defect created in rabbit animal model.
32084482	3	9	theme	swelling	648:655	arg1	2.6+-3.1					667:674	2.6+-3.1	667:674	2.6+-3.1	667:674	Developed scaffold exhibits controlled bioreactivity, minimize abrupt pH rise (~7.8), optimal swelling behavior (2.6+-3.1) and enhances mechanical strength (0.62 ± 0.18 MPa) under wet condition.
32084482	3	9	theme	swelling	648:655	arg1	behavior					657:664	optimal swelling behavior	640:664	optimal swelling behavior (2.6+-3.1)	640:675	Developed scaffold exhibits controlled bioreactivity, minimize abrupt pH rise (~7.8), optimal swelling behavior (2.6+-3.1) and enhances mechanical strength (0.62 ± 0.18 MPa) under wet condition.
32084482	3	9	theme	swelling	648:655	arg1	rise					627:630	abrupt pH rise	617:630	abrupt pH rise (~7.8)	617:637	Developed scaffold exhibits controlled bioreactivity, minimize abrupt pH rise (~7.8), optimal swelling behavior (2.6+-3.1) and enhances mechanical strength (0.62 ± 0.18 MPa) under wet condition.
32084482	0	10	theme	bone	111:114	arg1	engineering					123:133	bone tissue engineering	111:133	bone tissue engineering	111:133	Generation of scaffold incorporated with nanobioglass encapsulated in chitosan/chondroitin sulfate complex for bone tissue engineering.
32084482	2	11	theme	composite	407:415	arg1	scaffolds					417:425	nano-bioglass incorporated osteoconductive composite scaffolds	364:425	nano-bioglass incorporated osteoconductive composite scaffolds	364:425	In the present study, nano-bioglass incorporated osteoconductive composite scaffolds were fabricated through polyelectrolyte complexation/phase separation and resuspension of separated complex in gelatin matrix.
32084482	2	12	from	matrix	546:551	arg1	resuspension					501:512	resuspension	501:512	resuspension	501:512	In the present study, nano-bioglass incorporated osteoconductive composite scaffolds were fabricated through polyelectrolyte complexation/phase separation and resuspension of separated complex in gelatin matrix.
32084482	2	12	from	matrix	546:551	arg1	separation					486:495	polyelectrolyte complexation/phase separation	451:495	polyelectrolyte complexation/phase separation	451:495	In the present study, nano-bioglass incorporated osteoconductive composite scaffolds were fabricated through polyelectrolyte complexation/phase separation and resuspension of separated complex in gelatin matrix.
32084482	5	13	theme	rabbit	1102:1107	arg1	model					1116:1120	rabbit animal model	1102:1120	rabbit animal model	1102:1120	Also, the developed scaffold was evaluated for biocompatibility and bone tissue regeneration potential through implantation in non-union segmental bone defect created in rabbit animal model.
32084482	4	14	theme	in-vitro	759:766	arg1	study					773:777	in-vitro cell study	759:777	in-vitro cell study	759:777	Moreover, in-vitro cell study shows that the fabricated scaffold provide suitable template for cellular attachment, spreading, biomineralization and collagen based matrix deposition.
32084482	2	15	theme	complexation/phase	467:484	arg1	separation					486:495	polyelectrolyte complexation/phase separation	451:495	polyelectrolyte complexation/phase separation	451:495	In the present study, nano-bioglass incorporated osteoconductive composite scaffolds were fabricated through polyelectrolyte complexation/phase separation and resuspension of separated complex in gelatin matrix.
32084482	3	16	theme	Developed	554:562	arg1	scaffold					564:571	Developed scaffold	554:571	Developed scaffold	554:571	Developed scaffold exhibits controlled bioreactivity, minimize abrupt pH rise (~7.8), optimal swelling behavior (2.6+-3.1) and enhances mechanical strength (0.62 ± 0.18 MPa) under wet condition.
32084482	4	17	theme	based	907:911	arg1	deposition					920:929	collagen based matrix deposition	898:929	collagen based matrix deposition	898:929	Moreover, in-vitro cell study shows that the fabricated scaffold provide suitable template for cellular attachment, spreading, biomineralization and collagen based matrix deposition.
32084482	5	18	from	implantation	1043:1054	arg1	defect					1084:1089	non-union segmental bone defect	1059:1089	non-union segmental bone defect created in rabbit animal model	1059:1120	Also, the developed scaffold was evaluated for biocompatibility and bone tissue regeneration potential through implantation in non-union segmental bone defect created in rabbit animal model.
32084482	5	19	theme	non-union	1059:1067	arg1	defect					1084:1089	non-union segmental bone defect	1059:1089	non-union segmental bone defect created in rabbit animal model	1059:1120	Also, the developed scaffold was evaluated for biocompatibility and bone tissue regeneration potential through implantation in non-union segmental bone defect created in rabbit animal model.
32084482	2	20	theme	polyelectrolyte	451:465	arg1	separation					486:495	polyelectrolyte complexation/phase separation	451:495	polyelectrolyte complexation/phase separation	451:495	In the present study, nano-bioglass incorporated osteoconductive composite scaffolds were fabricated through polyelectrolyte complexation/phase separation and resuspension of separated complex in gelatin matrix.
32084482	2	21	theme	gelatin	538:544	arg1	matrix					546:551	gelatin matrix	538:551	gelatin matrix	538:551	In the present study, nano-bioglass incorporated osteoconductive composite scaffolds were fabricated through polyelectrolyte complexation/phase separation and resuspension of separated complex in gelatin matrix.
32084482	7	22	theme	tissue	1368:1373	arg1	applications					1387:1398	bone tissue engineering applications	1363:1398	bone tissue engineering applications	1363:1398	Thus, the developed composite scaffold might be a suitable biomaterial for bone tissue engineering applications.
32084482	7	23	theme	engineering	1375:1385	arg1	applications					1387:1398	bone tissue engineering applications	1363:1398	bone tissue engineering applications	1363:1398	Thus, the developed composite scaffold might be a suitable biomaterial for bone tissue engineering applications.
32084482	2	24	theme	present	349:355	arg1	study					357:361	the present study	345:361	the present study	345:361	In the present study, nano-bioglass incorporated osteoconductive composite scaffolds were fabricated through polyelectrolyte complexation/phase separation and resuspension of separated complex in gelatin matrix.
32084482	1	25	theme	natural	203:209	arg1	biopolymers					224:234	natural or synthetic biopolymers	203:234	natural or synthetic biopolymers incorporated with bioceramic	203:263	Over the past decade, various composite materials fabricated using natural or synthetic biopolymers incorporated with bioceramic have been widely investigated for the regeneration of segmental bone defect.
32084482	4	26	theme	suitable	822:829	arg1	template					831:838	suitable template	822:838	suitable template for cellular attachment, spreading, biomineralization and collagen based matrix deposition	822:929	Moreover, in-vitro cell study shows that the fabricated scaffold provide suitable template for cellular attachment, spreading, biomineralization and collagen based matrix deposition.
32084482	6	27	theme	scaffold	1202:1209	arg1	potential					1175:1183	strong potential	1168:1183	strong potential of the composite scaffold for bone tissue regeneration, vascularization and reconstruction of defects	1168:1285	The obtained histological analysis indicates strong potential of the composite scaffold for bone tissue regeneration, vascularization and reconstruction of defects.
32084482	2	28	theme	complex	527:533	arg1	resuspension					501:512	resuspension	501:512	resuspension	501:512	In the present study, nano-bioglass incorporated osteoconductive composite scaffolds were fabricated through polyelectrolyte complexation/phase separation and resuspension of separated complex in gelatin matrix.
32084482	2	28	theme	complex	527:533	arg1	separation					486:495	polyelectrolyte complexation/phase separation	451:495	polyelectrolyte complexation/phase separation	451:495	In the present study, nano-bioglass incorporated osteoconductive composite scaffolds were fabricated through polyelectrolyte complexation/phase separation and resuspension of separated complex in gelatin matrix.
32084482	1	29	theme	segmental	319:327	arg1	defect					334:339	segmental bone defect	319:339	segmental bone defect	319:339	Over the past decade, various composite materials fabricated using natural or synthetic biopolymers incorporated with bioceramic have been widely investigated for the regeneration of segmental bone defect.
32084482	3	30	theme	optimal	640:646	arg1	2.6+-3.1					667:674	2.6+-3.1	667:674	2.6+-3.1	667:674	Developed scaffold exhibits controlled bioreactivity, minimize abrupt pH rise (~7.8), optimal swelling behavior (2.6+-3.1) and enhances mechanical strength (0.62 ± 0.18 MPa) under wet condition.
32084482	3	30	theme	optimal	640:646	arg1	behavior					657:664	optimal swelling behavior	640:664	optimal swelling behavior (2.6+-3.1)	640:675	Developed scaffold exhibits controlled bioreactivity, minimize abrupt pH rise (~7.8), optimal swelling behavior (2.6+-3.1) and enhances mechanical strength (0.62 ± 0.18 MPa) under wet condition.
32084482	3	30	theme	optimal	640:646	arg1	rise					627:630	abrupt pH rise	617:630	abrupt pH rise (~7.8)	617:637	Developed scaffold exhibits controlled bioreactivity, minimize abrupt pH rise (~7.8), optimal swelling behavior (2.6+-3.1) and enhances mechanical strength (0.62 ± 0.18 MPa) under wet condition.
32084482	1	31	theme	synthetic	214:222	arg1	biopolymers					224:234	natural or synthetic biopolymers	203:234	natural or synthetic biopolymers incorporated with bioceramic	203:263	Over the past decade, various composite materials fabricated using natural or synthetic biopolymers incorporated with bioceramic have been widely investigated for the regeneration of segmental bone defect.
32084482	2	32	theme	separated	517:525	arg1	complex					527:533	separated complex	517:533	separated complex in gelatin matrix	517:551	In the present study, nano-bioglass incorporated osteoconductive composite scaffolds were fabricated through polyelectrolyte complexation/phase separation and resuspension of separated complex in gelatin matrix.
32084482	1	33	theme	bone	329:332	arg1	defect					334:339	segmental bone defect	319:339	segmental bone defect	319:339	Over the past decade, various composite materials fabricated using natural or synthetic biopolymers incorporated with bioceramic have been widely investigated for the regeneration of segmental bone defect.
32084482	5	34	theme	segmental	1069:1077	arg1	defect					1084:1089	non-union segmental bone defect	1059:1089	non-union segmental bone defect created in rabbit animal model	1059:1120	Also, the developed scaffold was evaluated for biocompatibility and bone tissue regeneration potential through implantation in non-union segmental bone defect created in rabbit animal model.
32084482	3	35	theme	wet	734:736	arg1	condition					738:746	wet condition	734:746	wet condition	734:746	Developed scaffold exhibits controlled bioreactivity, minimize abrupt pH rise (~7.8), optimal swelling behavior (2.6+-3.1) and enhances mechanical strength (0.62 ± 0.18 MPa) under wet condition.
32084482	3	36	theme	abrupt	617:622	arg1	behavior					657:664	optimal swelling behavior	640:664	optimal swelling behavior (2.6+-3.1)	640:675	Developed scaffold exhibits controlled bioreactivity, minimize abrupt pH rise (~7.8), optimal swelling behavior (2.6+-3.1) and enhances mechanical strength (0.62 ± 0.18 MPa) under wet condition.
32084482	3	36	theme	abrupt	617:622	arg1	~7.8					633:636	~7.8	633:636	~7.8	633:636	Developed scaffold exhibits controlled bioreactivity, minimize abrupt pH rise (~7.8), optimal swelling behavior (2.6+-3.1) and enhances mechanical strength (0.62 ± 0.18 MPa) under wet condition.
32084482	3	36	theme	abrupt	617:622	arg1	rise					627:630	abrupt pH rise	617:630	abrupt pH rise (~7.8)	617:637	Developed scaffold exhibits controlled bioreactivity, minimize abrupt pH rise (~7.8), optimal swelling behavior (2.6+-3.1) and enhances mechanical strength (0.62 ± 0.18 MPa) under wet condition.
32084482	1	37	theme	defect	334:339	arg1	regeneration					303:314	the regeneration	299:314	the regeneration of segmental bone defect	299:339	Over the past decade, various composite materials fabricated using natural or synthetic biopolymers incorporated with bioceramic have been widely investigated for the regeneration of segmental bone defect.
32084482	6	38	theme	defects	1279:1285	arg1	vascularization					1241:1255	vascularization	1241:1255	vascularization	1241:1255	The obtained histological analysis indicates strong potential of the composite scaffold for bone tissue regeneration, vascularization and reconstruction of defects.
32084482	6	38	theme	defects	1279:1285	arg1	regeneration					1227:1238	bone tissue regeneration	1215:1238	bone tissue regeneration	1215:1238	The obtained histological analysis indicates strong potential of the composite scaffold for bone tissue regeneration, vascularization and reconstruction of defects.
32084482	6	38	theme	defects	1279:1285	arg1	reconstruction					1261:1274	reconstruction	1261:1274	reconstruction	1261:1274	The obtained histological analysis indicates strong potential of the composite scaffold for bone tissue regeneration, vascularization and reconstruction of defects.
32084482	5	39	theme	developed	942:950	arg1	scaffold					952:959	the developed scaffold	938:959	the developed scaffold	938:959	Also, the developed scaffold was evaluated for biocompatibility and bone tissue regeneration potential through implantation in non-union segmental bone defect created in rabbit animal model.
32084482	6	40	theme	tissue	1220:1225	arg1	regeneration					1227:1238	bone tissue regeneration	1215:1238	bone tissue regeneration	1215:1238	The obtained histological analysis indicates strong potential of the composite scaffold for bone tissue regeneration, vascularization and reconstruction of defects.
32084482	7	41	theme	developed	1298:1306	arg1	biomaterial					1347:1357	a suitable biomaterial	1336:1357	a suitable biomaterial for bone tissue engineering applications	1336:1398	Thus, the developed composite scaffold might be a suitable biomaterial for bone tissue engineering applications.
32084482	7	41	theme	developed	1298:1306	arg1	scaffold					1318:1325	the developed composite scaffold	1294:1325	the developed composite scaffold	1294:1325	Thus, the developed composite scaffold might be a suitable biomaterial for bone tissue engineering applications.
32084482	4	42	theme	matrix	913:918	arg1	deposition					920:929	collagen based matrix deposition	898:929	collagen based matrix deposition	898:929	Moreover, in-vitro cell study shows that the fabricated scaffold provide suitable template for cellular attachment, spreading, biomineralization and collagen based matrix deposition.
32084482	7	43	theme	suitable	1338:1345	arg1	biomaterial					1347:1357	a suitable biomaterial	1336:1357	a suitable biomaterial for bone tissue engineering applications	1336:1398	Thus, the developed composite scaffold might be a suitable biomaterial for bone tissue engineering applications.
32084482	7	43	theme	suitable	1338:1345	arg1	scaffold					1318:1325	the developed composite scaffold	1294:1325	the developed composite scaffold	1294:1325	Thus, the developed composite scaffold might be a suitable biomaterial for bone tissue engineering applications.
32084482	2	44	theme	incorporated	378:389	arg1	scaffolds					417:425	nano-bioglass incorporated osteoconductive composite scaffolds	364:425	nano-bioglass incorporated osteoconductive composite scaffolds	364:425	In the present study, nano-bioglass incorporated osteoconductive composite scaffolds were fabricated through polyelectrolyte complexation/phase separation and resuspension of separated complex in gelatin matrix.
32084482	5	45	theme	bone	1079:1082	arg1	defect					1084:1089	non-union segmental bone defect	1059:1089	non-union segmental bone defect created in rabbit animal model	1059:1120	Also, the developed scaffold was evaluated for biocompatibility and bone tissue regeneration potential through implantation in non-union segmental bone defect created in rabbit animal model.
32084482	2	46	theme	nano-bioglass	364:376	arg1	scaffolds					417:425	nano-bioglass incorporated osteoconductive composite scaffolds	364:425	nano-bioglass incorporated osteoconductive composite scaffolds	364:425	In the present study, nano-bioglass incorporated osteoconductive composite scaffolds were fabricated through polyelectrolyte complexation/phase separation and resuspension of separated complex in gelatin matrix.
32084482	4	47	theme	collagen	898:905	arg1	deposition					920:929	collagen based matrix deposition	898:929	collagen based matrix deposition	898:929	Moreover, in-vitro cell study shows that the fabricated scaffold provide suitable template for cellular attachment, spreading, biomineralization and collagen based matrix deposition.
32084482	4	48	theme	fabricated	794:803	arg1	scaffold					805:812	the fabricated scaffold	790:812	the fabricated scaffold	790:812	Moreover, in-vitro cell study shows that the fabricated scaffold provide suitable template for cellular attachment, spreading, biomineralization and collagen based matrix deposition.
32084482	4	49	theme	cell	768:771	arg1	study					773:777	in-vitro cell study	759:777	in-vitro cell study	759:777	Moreover, in-vitro cell study shows that the fabricated scaffold provide suitable template for cellular attachment, spreading, biomineralization and collagen based matrix deposition.
32084482	1	50	theme	past	145:148	arg1	decade					150:155	the past decade	141:155	the past decade	141:155	Over the past decade, various composite materials fabricated using natural or synthetic biopolymers incorporated with bioceramic have been widely investigated for the regeneration of segmental bone defect.
32084482	4	51	theme	cellular	844:851	arg1	attachment					853:862	cellular attachment	844:862	cellular attachment	844:862	Moreover, in-vitro cell study shows that the fabricated scaffold provide suitable template for cellular attachment, spreading, biomineralization and collagen based matrix deposition.
32084482	6	52	theme	strong	1168:1173	arg1	potential					1175:1183	strong potential	1168:1183	strong potential of the composite scaffold for bone tissue regeneration, vascularization and reconstruction of defects	1168:1285	The obtained histological analysis indicates strong potential of the composite scaffold for bone tissue regeneration, vascularization and reconstruction of defects.
32084482	5	53	theme	animal	1109:1114	arg1	model					1116:1120	rabbit animal model	1102:1120	rabbit animal model	1102:1120	Also, the developed scaffold was evaluated for biocompatibility and bone tissue regeneration potential through implantation in non-union segmental bone defect created in rabbit animal model.
32084482	5	54	theme	potential	1025:1033	arg1	regeneration					1012:1023	bone tissue regeneration	1000:1023	bone tissue regeneration potential through implantation in non-union segmental bone defect created in rabbit animal model	1000:1120	Also, the developed scaffold was evaluated for biocompatibility and bone tissue regeneration potential through implantation in non-union segmental bone defect created in rabbit animal model.
32084482	3	55	theme	controlled	582:591	arg1	bioreactivity					593:605	controlled bioreactivity	582:605	controlled bioreactivity	582:605	Developed scaffold exhibits controlled bioreactivity, minimize abrupt pH rise (~7.8), optimal swelling behavior (2.6+-3.1) and enhances mechanical strength (0.62 ± 0.18 MPa) under wet condition.
32084482	7	56	theme	composite	1308:1316	arg1	biomaterial					1347:1357	a suitable biomaterial	1336:1357	a suitable biomaterial for bone tissue engineering applications	1336:1398	Thus, the developed composite scaffold might be a suitable biomaterial for bone tissue engineering applications.
32084482	7	56	theme	composite	1308:1316	arg1	scaffold					1318:1325	the developed composite scaffold	1294:1325	the developed composite scaffold	1294:1325	Thus, the developed composite scaffold might be a suitable biomaterial for bone tissue engineering applications.
32084482	0	57	theme	sulfate	91:97	arg1	complex					99:105	chitosan/chondroitin sulfate complex	70:105	chitosan/chondroitin sulfate complex for bone tissue engineering	70:133	Generation of scaffold incorporated with nanobioglass encapsulated in chitosan/chondroitin sulfate complex for bone tissue engineering.
32084482	3	58	theme	mechanical	690:699	arg1	strength					701:708	mechanical strength	690:708	mechanical strength (0.62 ± 0.18 MPa)	690:726	Developed scaffold exhibits controlled bioreactivity, minimize abrupt pH rise (~7.8), optimal swelling behavior (2.6+-3.1) and enhances mechanical strength (0.62 ± 0.18 MPa) under wet condition.
32084482	3	58	theme	mechanical	690:699	arg1	0.62 ± 0.18 MPa					711:725	0.62 ± 0.18 MPa	711:725	0.62 ± 0.18 MPa	711:725	Developed scaffold exhibits controlled bioreactivity, minimize abrupt pH rise (~7.8), optimal swelling behavior (2.6+-3.1) and enhances mechanical strength (0.62 ± 0.18 MPa) under wet condition.
32084482	2	59	from	separation	486:495	arg1	matrix					546:551	gelatin matrix	538:551	gelatin matrix	538:551	In the present study, nano-bioglass incorporated osteoconductive composite scaffolds were fabricated through polyelectrolyte complexation/phase separation and resuspension of separated complex in gelatin matrix.
32084482	3	60	theme	pH	624:625	arg1	behavior					657:664	optimal swelling behavior	640:664	optimal swelling behavior (2.6+-3.1)	640:675	Developed scaffold exhibits controlled bioreactivity, minimize abrupt pH rise (~7.8), optimal swelling behavior (2.6+-3.1) and enhances mechanical strength (0.62 ± 0.18 MPa) under wet condition.
32084482	3	60	theme	pH	624:625	arg1	~7.8					633:636	~7.8	633:636	~7.8	633:636	Developed scaffold exhibits controlled bioreactivity, minimize abrupt pH rise (~7.8), optimal swelling behavior (2.6+-3.1) and enhances mechanical strength (0.62 ± 0.18 MPa) under wet condition.
32084482	3	60	theme	pH	624:625	arg1	rise					627:630	abrupt pH rise	617:630	abrupt pH rise (~7.8)	617:637	Developed scaffold exhibits controlled bioreactivity, minimize abrupt pH rise (~7.8), optimal swelling behavior (2.6+-3.1) and enhances mechanical strength (0.62 ± 0.18 MPa) under wet condition.
32084482	6	61	theme	bone	1215:1218	arg1	regeneration					1227:1238	bone tissue regeneration	1215:1238	bone tissue regeneration	1215:1238	The obtained histological analysis indicates strong potential of the composite scaffold for bone tissue regeneration, vascularization and reconstruction of defects.
32084482	0	62	theme	chitosan/chondroitin	70:89	arg1	complex					99:105	chitosan/chondroitin sulfate complex	70:105	chitosan/chondroitin sulfate complex for bone tissue engineering	70:133	Generation of scaffold incorporated with nanobioglass encapsulated in chitosan/chondroitin sulfate complex for bone tissue engineering.
32084482	2	63	from	complex	527:533	arg1	matrix					546:551	gelatin matrix	538:551	gelatin matrix	538:551	In the present study, nano-bioglass incorporated osteoconductive composite scaffolds were fabricated through polyelectrolyte complexation/phase separation and resuspension of separated complex in gelatin matrix.
32084482	0	64	theme	scaffold	14:21	arg1	Generation					0:9	Generation	0:9	Generation of scaffold	0:21	Generation of scaffold incorporated with nanobioglass encapsulated in chitosan/chondroitin sulfate complex for bone tissue engineering.
32084482	1	65	theme	various	158:164	arg1	materials					176:184	various composite materials	158:184	various composite materials fabricated using natural or synthetic biopolymers incorporated with bioceramic	158:263	Over the past decade, various composite materials fabricated using natural or synthetic biopolymers incorporated with bioceramic have been widely investigated for the regeneration of segmental bone defect.
32084482	6	66	theme	histological	1136:1147	arg1	analysis					1149:1156	The obtained histological analysis	1123:1156	The obtained histological analysis	1123:1156	The obtained histological analysis indicates strong potential of the composite scaffold for bone tissue regeneration, vascularization and reconstruction of defects.
31410767	1	0	theme	biological	340:349	arg1	properties					351:360	their diverse physicochemical and biological properties	306:360	their diverse physicochemical and biological properties	306:360	Growth media composition is a critical factor influencing the yield of bacterial exopolysaccharides (EPSs), which have attracted the interest of researchers around the world due to their diverse physicochemical and biological properties.
31410767	3	1	theme	rhamnosus	662:670	arg1	ŁOCK					672:675	L. rhamnosus ŁOCK 0935	659:680	L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L)	659:706	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	4	2	theme	medium-related	901:914	arg1	case					1011:1014	the carbon source-in this case	985:1014	the carbon source-in this case	985:1014	It has been found that the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains are the carbon source-in this case, it was fructose and sucrose.
31410767	4	2	theme	medium-related	901:914	arg1	determinant					916:926	the main medium-related determinant	892:926	the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains	892:979	It has been found that the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains are the carbon source-in this case, it was fructose and sucrose.
31410767	2	3	theme	ŁOCK	486:489	arg1	strains					459:465	three Lactobacillus rhamnosus strains	429:465	three Lactobacillus rhamnosus strains	429:465	This work presents the optimization of media for EPS synthesis by three Lactobacillus rhamnosus strains, namely ŁOCK 0943, ŁOCK 0935, and OM-1.
31410767	2	3	theme	ŁOCK	486:489	arg1	0935					491:494	ŁOCK 0935	486:494	ŁOCK 0935	486:494	This work presents the optimization of media for EPS synthesis by three Lactobacillus rhamnosus strains, namely ŁOCK 0943, ŁOCK 0935, and OM-1.
31410767	4	4	theme	EPS	931:933	arg1	synthesis					935:943	EPS synthesis	931:943	EPS synthesis by the studied L. rhamnosus strains	931:979	It has been found that the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains are the carbon source-in this case, it was fructose and sucrose.
31410767	0	5	theme	rhamnosus	106:114	arg1	Strains					116:122	Lactobacillus rhamnosus Strains	92:122	Lactobacillus rhamnosus Strains	92:122	Optimization of Media Composition to Maximize the Yield of Exopolysaccharides Production by Lactobacillus rhamnosus Strains.
31410767	3	6	theme	9-fold	639:644	arg1	increase					646:653	an almost 9-fold increase	629:653	an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L)	629:706	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	0	7	theme	Lactobacillus	92:104	arg1	Strains					116:122	Lactobacillus rhamnosus Strains	92:122	Lactobacillus rhamnosus Strains	92:122	Optimization of Media Composition to Maximize the Yield of Exopolysaccharides Production by Lactobacillus rhamnosus Strains.
31410767	4	8	theme	carbon	989:994	arg1	case					1011:1014	the carbon source-in this case	985:1014	the carbon source-in this case	985:1014	It has been found that the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains are the carbon source-in this case, it was fructose and sucrose.
31410767	4	8	theme	carbon	989:994	arg1	determinant					916:926	the main medium-related determinant	892:926	the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains	892:979	It has been found that the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains are the carbon source-in this case, it was fructose and sucrose.
31410767	1	9	theme	researchers	270:280	arg1	interest					258:265	the interest	254:265	the interest of researchers around the world	254:297	Growth media composition is a critical factor influencing the yield of bacterial exopolysaccharides (EPSs), which have attracted the interest of researchers around the world due to their diverse physicochemical and biological properties.
31410767	3	10	from	85	609:610	arg1	0943					598:601	L. rhamnosus ŁOCK 0943	580:601	L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L)	580:626	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	3	11	dep	85	609:610	arg1	to					612:613	to	612:613	to	612:613	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	3	11	dep	85	609:610	arg1	1138.2 mg/L					615:625	1138.2 mg/L	615:625	85 to 1138.2 mg/L	609:625	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	3	12	theme	L.	659:660	arg1	ŁOCK					672:675	L. rhamnosus ŁOCK 0935	659:680	L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L)	659:706	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	2	13	theme	media	402:406	arg1	optimization					386:397	the optimization	382:397	the optimization of media for EPS synthesis by three Lactobacillus rhamnosus strains, namely ŁOCK 0943, ŁOCK 0935, and OM-1	382:504	This work presents the optimization of media for EPS synthesis by three Lactobacillus rhamnosus strains, namely ŁOCK 0943, ŁOCK 0935, and OM-1.
31410767	0	14	theme	Production	78:87	arg1	Yield					50:54	the Yield	46:54	the Yield of Exopolysaccharides Production by Lactobacillus rhamnosus Strains	46:122	Optimization of Media Composition to Maximize the Yield of Exopolysaccharides Production by Lactobacillus rhamnosus Strains.
31410767	3	15	from	103.67	688:693	arg1	ŁOCK					672:675	L. rhamnosus ŁOCK 0935	659:680	L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L)	659:706	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	3	16	dep	987.84 mg/L	779:789	arg1	133.67					769:774	133.67	769:774	133.67	769:774	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	4	17	theme	source-in	996:1004	arg1	case					1011:1014	the carbon source-in this case	985:1014	the carbon source-in this case	985:1014	It has been found that the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains are the carbon source-in this case, it was fructose and sucrose.
31410767	4	17	theme	source-in	996:1004	arg1	determinant					916:926	the main medium-related determinant	892:926	the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains	892:979	It has been found that the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains are the carbon source-in this case, it was fructose and sucrose.
31410767	1	18	theme	bacterial	196:204	arg1	EPSs					226:229	EPSs	226:229	EPSs	226:229	Growth media composition is a critical factor influencing the yield of bacterial exopolysaccharides (EPSs), which have attracted the interest of researchers around the world due to their diverse physicochemical and biological properties.
31410767	1	18	theme	bacterial	196:204	arg1	exopolysaccharides					206:223	bacterial exopolysaccharides	196:223	bacterial exopolysaccharides (EPSs)	196:230	Growth media composition is a critical factor influencing the yield of bacterial exopolysaccharides (EPSs), which have attracted the interest of researchers around the world due to their diverse physicochemical and biological properties.
31410767	2	19	theme	rhamnosus	449:457	arg1	strains					459:465	three Lactobacillus rhamnosus strains	429:465	three Lactobacillus rhamnosus strains	429:465	This work presents the optimization of media for EPS synthesis by three Lactobacillus rhamnosus strains, namely ŁOCK 0943, ŁOCK 0935, and OM-1.
31410767	2	19	theme	rhamnosus	449:457	arg1	0943					480:483	ŁOCK 0943	475:483	ŁOCK 0943	475:483	This work presents the optimization of media for EPS synthesis by three Lactobacillus rhamnosus strains, namely ŁOCK 0943, ŁOCK 0935, and OM-1.
31410767	2	19	theme	rhamnosus	449:457	arg1	0935					491:494	ŁOCK 0935	486:494	ŁOCK 0935	486:494	This work presents the optimization of media for EPS synthesis by three Lactobacillus rhamnosus strains, namely ŁOCK 0943, ŁOCK 0935, and OM-1.
31410767	2	19	theme	rhamnosus	449:457	arg1	OM-1					501:504	OM-1	501:504	OM-1	501:504	This work presents the optimization of media for EPS synthesis by three Lactobacillus rhamnosus strains, namely ŁOCK 0943, ŁOCK 0935, and OM-1.
31410767	3	20	from	cultures	807:814	arg1	medium					832:837	standard MRS medium	819:837	standard MRS medium (de Man, Rogosa, and Sharpe)	819:866	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	3	21	from	increase	732:739	arg1	yield					570:574	EPS yield	566:574	EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L)	566:626	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	3	22	theme	more	536:539	arg1	OM-1					758:761	a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1	534:761	a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L)	534:790	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	4	23	theme	L.	960:961	arg1	strains					973:979	the studied L. rhamnosus strains	948:979	the studied L. rhamnosus strains	948:979	It has been found that the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains are the carbon source-in this case, it was fructose and sucrose.
31410767	1	24	theme	exopolysaccharides	206:223	arg1	yield					187:191	the yield	183:191	the yield of bacterial exopolysaccharides (EPSs)	183:230	Growth media composition is a critical factor influencing the yield of bacterial exopolysaccharides (EPSs), which have attracted the interest of researchers around the world due to their diverse physicochemical and biological properties.
31410767	2	25	theme	Lactobacillus	435:447	arg1	strains					459:465	three Lactobacillus rhamnosus strains	429:465	three Lactobacillus rhamnosus strains	429:465	This work presents the optimization of media for EPS synthesis by three Lactobacillus rhamnosus strains, namely ŁOCK 0943, ŁOCK 0935, and OM-1.
31410767	2	25	theme	Lactobacillus	435:447	arg1	0943					480:483	ŁOCK 0943	475:483	ŁOCK 0943	475:483	This work presents the optimization of media for EPS synthesis by three Lactobacillus rhamnosus strains, namely ŁOCK 0943, ŁOCK 0935, and OM-1.
31410767	2	25	theme	Lactobacillus	435:447	arg1	0935					491:494	ŁOCK 0935	486:494	ŁOCK 0935	486:494	This work presents the optimization of media for EPS synthesis by three Lactobacillus rhamnosus strains, namely ŁOCK 0943, ŁOCK 0935, and OM-1.
31410767	2	25	theme	Lactobacillus	435:447	arg1	OM-1					501:504	OM-1	501:504	OM-1	501:504	This work presents the optimization of media for EPS synthesis by three Lactobacillus rhamnosus strains, namely ŁOCK 0943, ŁOCK 0935, and OM-1.
31410767	4	26	dep	case	1011:1014	arg1	sucrose					1037:1043	sucrose	1037:1043	sucrose	1037:1043	It has been found that the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains are the carbon source-in this case, it was fructose and sucrose.
31410767	4	26	dep	case	1011:1014	arg1	fructose					1024:1031	fructose	1024:1031	fructose	1024:1031	It has been found that the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains are the carbon source-in this case, it was fructose and sucrose.
31410767	3	27	theme	standard	819:826	arg1	medium					832:837	standard MRS medium	819:837	standard MRS medium (de Man, Rogosa, and Sharpe)	819:866	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	4	28	theme	main	896:899	arg1	case					1011:1014	the carbon source-in this case	985:1014	the carbon source-in this case	985:1014	It has been found that the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains are the carbon source-in this case, it was fructose and sucrose.
31410767	4	28	theme	main	896:899	arg1	determinant					916:926	the main medium-related determinant	892:926	the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains	892:979	It has been found that the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains are the carbon source-in this case, it was fructose and sucrose.
31410767	3	29	theme	MRS	828:830	arg1	medium					832:837	standard MRS medium	819:837	standard MRS medium (de Man, Rogosa, and Sharpe)	819:866	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	0	30	theme	Media	16:20	arg1	Composition					22:32	Media Composition	16:32	Media Composition	16:32	Optimization of Media Composition to Maximize the Yield of Exopolysaccharides Production by Lactobacillus rhamnosus Strains.
31410767	0	31	theme	Composition	22:32	arg1	Optimization					0:11	Optimization	0:11	Optimization of Media Composition to Maximize the Yield of Exopolysaccharides Production by Lactobacillus rhamnosus Strains.	0:123	Optimization of Media Composition to Maximize the Yield of Exopolysaccharides Production by Lactobacillus rhamnosus Strains.
31410767	2	32	theme	EPS	412:414	arg1	synthesis					416:424	EPS synthesis	412:424	EPS synthesis by three Lactobacillus rhamnosus strains, namely ŁOCK 0943, ŁOCK 0935, and OM-1	412:504	This work presents the optimization of media for EPS synthesis by three Lactobacillus rhamnosus strains, namely ŁOCK 0943, ŁOCK 0935, and OM-1.
31410767	2	33	theme	ŁOCK	475:478	arg1	strains					459:465	three Lactobacillus rhamnosus strains	429:465	three Lactobacillus rhamnosus strains	429:465	This work presents the optimization of media for EPS synthesis by three Lactobacillus rhamnosus strains, namely ŁOCK 0943, ŁOCK 0935, and OM-1.
31410767	2	33	theme	ŁOCK	475:478	arg1	0943					480:483	ŁOCK 0943	475:483	ŁOCK 0943	475:483	This work presents the optimization of media for EPS synthesis by three Lactobacillus rhamnosus strains, namely ŁOCK 0943, ŁOCK 0935, and OM-1.
31410767	3	34	dep	7-fold	725:730	arg1	than					720:723	than	720:723	than	720:723	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	3	34	dep	7-fold	725:730	arg1	more					715:718	more	715:718	more	715:718	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	1	35	theme	Growth	125:130	arg1	media					132:136	Growth media	125:136	Growth media composition	125:148	Growth media composition is a critical factor influencing the yield of bacterial exopolysaccharides (EPSs), which have attracted the interest of researchers around the world due to their diverse physicochemical and biological properties.
31410767	1	36	theme	diverse	312:318	arg1	properties					351:360	their diverse physicochemical and biological properties	306:360	their diverse physicochemical and biological properties	306:360	Growth media composition is a critical factor influencing the yield of bacterial exopolysaccharides (EPSs), which have attracted the interest of researchers around the world due to their diverse physicochemical and biological properties.
31410767	3	37	theme	EPS	566:568	arg1	yield					570:574	EPS yield	566:574	EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L)	566:626	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	3	38	theme	de	840:841	arg1	Man					843:845	de Man	840:845	de Man	840:845	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	1	39	theme	media	132:136	arg1	composition					138:148	Growth media composition	125:148	Growth media composition	125:148	Growth media composition is a critical factor influencing the yield of bacterial exopolysaccharides (EPSs), which have attracted the interest of researchers around the world due to their diverse physicochemical and biological properties.
31410767	1	39	theme	media	132:136	arg1	factor					164:169	a critical factor	153:169	a critical factor	153:169	Growth media composition is a critical factor influencing the yield of bacterial exopolysaccharides (EPSs), which have attracted the interest of researchers around the world due to their diverse physicochemical and biological properties.
31410767	1	40	theme	physicochemical	320:334	arg1	properties					351:360	their diverse physicochemical and biological properties	306:360	their diverse physicochemical and biological properties	306:360	Growth media composition is a critical factor influencing the yield of bacterial exopolysaccharides (EPSs), which have attracted the interest of researchers around the world due to their diverse physicochemical and biological properties.
31410767	3	41	theme	ŁOCK	593:596	arg1	0943					598:601	L. rhamnosus ŁOCK 0943	580:601	L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L)	580:626	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	4	42	dep	L.	960:961	arg1	rhamnosus					963:971	L. rhamnosus	960:971	the studied L. rhamnosus strains	948:979	It has been found that the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains are the carbon source-in this case, it was fructose and sucrose.
31410767	4	43	theme	synthesis	935:943	arg1	case					1011:1014	the carbon source-in this case	985:1014	the carbon source-in this case	985:1014	It has been found that the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains are the carbon source-in this case, it was fructose and sucrose.
31410767	4	43	theme	synthesis	935:943	arg1	determinant					916:926	the main medium-related determinant	892:926	the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains	892:979	It has been found that the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains are the carbon source-in this case, it was fructose and sucrose.
31410767	3	44	theme	optimized	511:519	arg1	media					521:525	The optimized media	507:525	The optimized media	507:525	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	4	45	theme	studied	952:958	arg1	strains					973:979	the studied L. rhamnosus strains	948:979	the studied L. rhamnosus strains	948:979	It has been found that the main medium-related determinant of EPS synthesis by the studied L. rhamnosus strains are the carbon source-in this case, it was fructose and sucrose.
31410767	3	46	theme	7-fold	725:730	arg1	increase					732:739	a more than 7-fold increase	713:739	a more than 7-fold increase	713:739	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	0	47	theme	Exopolysaccharides	59:76	arg1	Production					78:87	Exopolysaccharides Production	59:87	Exopolysaccharides Production	59:87	Optimization of Media Composition to Maximize the Yield of Exopolysaccharides Production by Lactobacillus rhamnosus Strains.
31410767	3	48	theme	L.	580:581	arg1	0943					598:601	L. rhamnosus ŁOCK 0943	580:601	L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L)	580:626	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	3	49	from	increase	554:561	arg1	yield					570:574	EPS yield	566:574	EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L)	566:626	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	3	50	theme	rhamnosus	583:591	arg1	0943					598:601	L. rhamnosus ŁOCK 0943	580:601	L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L)	580:626	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	3	51	from	increase	646:653	arg1	yield					570:574	EPS yield	566:574	EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L)	566:626	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	0	52	dep	Optimization	0:11	arg1	Maximize					37:44	Maximize	37:44	to Maximize the Yield of Exopolysaccharides Production by Lactobacillus rhamnosus Strains	34:122	Optimization of Media Composition to Maximize the Yield of Exopolysaccharides Production by Lactobacillus rhamnosus Strains.
31410767	3	53	dep	medium	832:837	arg1	Rogosa					848:853	Rogosa	848:853	Rogosa	848:853	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	3	53	dep	medium	832:837	arg1	Sharpe					860:865	Sharpe	860:865	Sharpe	860:865	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	3	53	dep	medium	832:837	arg1	Man					843:845	de Man	840:845	de Man	840:845	The optimized media led to a more than 13-fold increase in EPS yield for L. rhamnosus ŁOCK 0943 (from 85 to 1138.2 mg/L), an almost 9-fold increase for L. rhamnosus ŁOCK 0935 (from 103.67 to 900 mg/L), and a more than 7-fold increase for L. rhamnosus OM-1 (from 133.67 to 987.84 mg/L) as compared to cultures in standard MRS medium (de Man, Rogosa, and Sharpe).
31410767	1	54	theme	critical	155:162	arg1	composition					138:148	Growth media composition	125:148	Growth media composition	125:148	Growth media composition is a critical factor influencing the yield of bacterial exopolysaccharides (EPSs), which have attracted the interest of researchers around the world due to their diverse physicochemical and biological properties.
31410767	1	54	theme	critical	155:162	arg1	factor					164:169	a critical factor	153:169	a critical factor	153:169	Growth media composition is a critical factor influencing the yield of bacterial exopolysaccharides (EPSs), which have attracted the interest of researchers around the world due to their diverse physicochemical and biological properties.
31929075	0	0	theme	Ce-ZnO	83:88	arg1	nano-flowers					90:101	Ce-ZnO nano-flowers	83:101	Ce-ZnO nano-flowers	83:101	Photocatalytic degradation of malachite green dye using chitosan supported ZnO and Ce-ZnO nano-flowers under visible light.
31929075	2	1	from	flowers	453:459	arg1	ZnO					431:433	ZnO and Ce doped ZnO	414:433	ZnO and Ce doped ZnO at stunning micro flowers of nano limps	414:473	The morphological investigation reflected the formation of ZnO and Ce doped ZnO at stunning micro flowers of nano limps.
31929075	2	1	from	flowers	453:459	arg1	formation					401:409	the formation	397:409	the formation of ZnO and Ce doped ZnO at stunning micro flowers of nano limps	397:473	The morphological investigation reflected the formation of ZnO and Ce doped ZnO at stunning micro flowers of nano limps.
31929075	4	2	theme	photocatalytic	717:730	arg1	removal					732:738	photocatalytic removal	717:738	photocatalytic removal of malachite green dye	717:761	The synthetic composites were applied in photocatalytic removal of malachite green dye under a visible light source.
31929075	3	3	from	3.3 eV	559:564	arg1	bandgap					539:545	the bandgap	535:545	the bandgap of ZnO from 3.3 eV to 2.85 eV and 2.5 eV	535:586	Additionally, the optical studies reflected a reduction in the bandgap of ZnO from 3.3 eV to 2.85 eV and 2.5 eV after supporting it onto chitosan chains and after doping it with cerium, respectively.
31929075	1	4	theme	advanced	275:282	arg1	types					128:132	Two types	124:132	Two types of chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites)	124:207	Two types of chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites) were synthesized under microwave irradiation and characterized as advanced catalysts of enhanced photocatalytic activity under the visible light.
31929075	1	4	theme	advanced	275:282	arg1	catalysts					284:292	advanced catalysts	275:292	advanced catalysts of enhanced photocatalytic activity under the visible light	275:352	Two types of chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites) were synthesized under microwave irradiation and characterized as advanced catalysts of enhanced photocatalytic activity under the visible light.
31929075	7	5	theme	hydroxyl	1201:1208	arg1	radicals					1210:1217	the hydroxyl radicals	1197:1217	the generated electron-hole pairs as well as the hydroxyl radicals	1152:1217	The main oxidizing radicals during the removal of M.G by CH/ZnO are the generated electron-hole pairs as well as the hydroxyl radicals.
31929075	1	6	theme	chitosan/ZnO	164:175	arg1	composites					197:206	chitosan/ZnO and chitosan/Ce-ZnO composites	164:206	chitosan/ZnO and chitosan/Ce-ZnO composites	164:206	Two types of chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites) were synthesized under microwave irradiation and characterized as advanced catalysts of enhanced photocatalytic activity under the visible light.
31929075	1	6	theme	chitosan/ZnO	164:175	arg1	composites					152:161	chitosan-based composites	137:161	chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites)	137:207	Two types of chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites) were synthesized under microwave irradiation and characterized as advanced catalysts of enhanced photocatalytic activity under the visible light.
31929075	8	7	theme	effective	1224:1232	arg1	species					1234:1240	The effective species	1220:1240	The effective species in CH/Ce-ZnO photocatalytic system	1220:1275	The effective species in CH/Ce-ZnO photocatalytic system are the photogenerated hydroxyl radicals followed by the electron-hole pairs.
31929075	8	7	theme	effective	1224:1232	arg1	radicals					1309:1316	the photogenerated hydroxyl radicals	1281:1316	the photogenerated hydroxyl radicals followed by the electron-hole pairs	1281:1352	The effective species in CH/Ce-ZnO photocatalytic system are the photogenerated hydroxyl radicals followed by the electron-hole pairs.
31929075	3	8	from	bandgap	539:545	arg1	3.3 eV					559:564	3.3 eV	559:564	3.3 eV	559:564	Additionally, the optical studies reflected a reduction in the bandgap of ZnO from 3.3 eV to 2.85 eV and 2.5 eV after supporting it onto chitosan chains and after doping it with cerium, respectively.
31929075	3	9	dep	reflected	510:518	arg1	doping					639:644	doping	639:644	doping	639:644	Additionally, the optical studies reflected a reduction in the bandgap of ZnO from 3.3 eV to 2.85 eV and 2.5 eV after supporting it onto chitosan chains and after doping it with cerium, respectively.
31929075	3	9	dep	reflected	510:518	arg1	supporting					594:603	supporting	594:603	supporting it onto chitosan chains	594:627	Additionally, the optical studies reflected a reduction in the bandgap of ZnO from 3.3 eV to 2.85 eV and 2.5 eV after supporting it onto chitosan chains and after doping it with cerium, respectively.
31929075	6	10	theme	high	975:978	arg1	stability					980:988	high stability	975:988	high stability	975:988	The synthetic composites are of high stability and can be reused for five photocatalytic degradation cycles at stunning removal percentages.
31929075	4	11	theme	light	779:783	arg1	source					785:790	a visible light source	769:790	a visible light source	769:790	The synthetic composites were applied in photocatalytic removal of malachite green dye under a visible light source.
31929075	1	12	theme	enhanced	297:304	arg1	activity					321:328	enhanced photocatalytic activity	297:328	enhanced photocatalytic activity under the visible light	297:352	Two types of chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites) were synthesized under microwave irradiation and characterized as advanced catalysts of enhanced photocatalytic activity under the visible light.
31929075	7	13	theme	main	1088:1091	arg1	pairs					1180:1184	the generated electron-hole pairs	1152:1184	the generated electron-hole pairs as well as the hydroxyl radicals	1152:1217	The main oxidizing radicals during the removal of M.G by CH/ZnO are the generated electron-hole pairs as well as the hydroxyl radicals.
31929075	7	13	theme	main	1088:1091	arg1	radicals					1103:1110	The main oxidizing radicals	1084:1110	The main oxidizing radicals during the removal of M.G by CH/ZnO	1084:1146	The main oxidizing radicals during the removal of M.G by CH/ZnO are the generated electron-hole pairs as well as the hydroxyl radicals.
31929075	8	14	theme	CH/Ce-ZnO	1245:1253	arg1	system					1270:1275	CH/Ce-ZnO photocatalytic system	1245:1275	CH/Ce-ZnO photocatalytic system	1245:1275	The effective species in CH/Ce-ZnO photocatalytic system are the photogenerated hydroxyl radicals followed by the electron-hole pairs.
31929075	8	15	theme	photocatalytic	1255:1268	arg1	system					1270:1275	CH/Ce-ZnO photocatalytic system	1245:1275	CH/Ce-ZnO photocatalytic system	1245:1275	The effective species in CH/Ce-ZnO photocatalytic system are the photogenerated hydroxyl radicals followed by the electron-hole pairs.
31929075	6	16	theme	stunning	1054:1061	arg1	percentages					1071:1081	stunning removal percentages	1054:1081	stunning removal percentages	1054:1081	The synthetic composites are of high stability and can be reused for five photocatalytic degradation cycles at stunning removal percentages.
31929075	8	17	theme	electron-hole	1334:1346	arg1	pairs					1348:1352	the electron-hole pairs	1330:1352	the electron-hole pairs	1330:1352	The effective species in CH/Ce-ZnO photocatalytic system are the photogenerated hydroxyl radicals followed by the electron-hole pairs.
31929075	0	18	theme	visible	109:115	arg1	light					117:121	visible light	109:121	visible light	109:121	Photocatalytic degradation of malachite green dye using chitosan supported ZnO and Ce-ZnO nano-flowers under visible light.
31929075	7	19	theme	oxidizing	1093:1101	arg1	pairs					1180:1184	the generated electron-hole pairs	1152:1184	the generated electron-hole pairs as well as the hydroxyl radicals	1152:1217	The main oxidizing radicals during the removal of M.G by CH/ZnO are the generated electron-hole pairs as well as the hydroxyl radicals.
31929075	7	19	theme	oxidizing	1093:1101	arg1	radicals					1103:1110	The main oxidizing radicals	1084:1110	The main oxidizing radicals during the removal of M.G by CH/ZnO	1084:1146	The main oxidizing radicals during the removal of M.G by CH/ZnO are the generated electron-hole pairs as well as the hydroxyl radicals.
31929075	2	20	theme	morphological	359:371	arg1	investigation					373:385	The morphological investigation	355:385	The morphological investigation	355:385	The morphological investigation reflected the formation of ZnO and Ce doped ZnO at stunning micro flowers of nano limps.
31929075	2	21	theme	ZnO	431:433	arg1	formation					401:409	the formation	397:409	the formation of ZnO and Ce doped ZnO at stunning micro flowers of nano limps	397:473	The morphological investigation reflected the formation of ZnO and Ce doped ZnO at stunning micro flowers of nano limps.
31929075	7	22	theme	generated	1156:1164	arg1	pairs					1180:1184	the generated electron-hole pairs	1152:1184	the generated electron-hole pairs as well as the hydroxyl radicals	1152:1217	The main oxidizing radicals during the removal of M.G by CH/ZnO are the generated electron-hole pairs as well as the hydroxyl radicals.
31929075	7	22	theme	generated	1156:1164	arg1	radicals					1103:1110	The main oxidizing radicals	1084:1110	The main oxidizing radicals during the removal of M.G by CH/ZnO	1084:1146	The main oxidizing radicals during the removal of M.G by CH/ZnO are the generated electron-hole pairs as well as the hydroxyl radicals.
31929075	3	23	theme	ZnO	550:552	arg1	bandgap					539:545	the bandgap	535:545	the bandgap of ZnO from 3.3 eV to 2.85 eV and 2.5 eV	535:586	Additionally, the optical studies reflected a reduction in the bandgap of ZnO from 3.3 eV to 2.85 eV and 2.5 eV after supporting it onto chitosan chains and after doping it with cerium, respectively.
31929075	2	24	from	formation	401:409	arg1	flowers					453:459	stunning micro flowers	438:459	stunning micro flowers of nano limps	438:473	The morphological investigation reflected the formation of ZnO and Ce doped ZnO at stunning micro flowers of nano limps.
31929075	2	25	theme	doped	425:429	arg1	ZnO					431:433	ZnO and Ce doped ZnO	414:433	ZnO and Ce doped ZnO at stunning micro flowers of nano limps	414:473	The morphological investigation reflected the formation of ZnO and Ce doped ZnO at stunning micro flowers of nano limps.
31929075	0	26	theme	Photocatalytic	0:13	arg1	degradation					15:25	Photocatalytic degradation	0:25	Photocatalytic degradation of malachite green dye using chitosan	0:63	Photocatalytic degradation of malachite green dye using chitosan supported ZnO and Ce-ZnO nano-flowers under visible light.
31929075	1	27	theme	photocatalytic	306:319	arg1	activity					321:328	enhanced photocatalytic activity	297:328	enhanced photocatalytic activity under the visible light	297:352	Two types of chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites) were synthesized under microwave irradiation and characterized as advanced catalysts of enhanced photocatalytic activity under the visible light.
31929075	4	28	theme	malachite	743:751	arg1	green					753:757	malachite green dye	743:761	malachite green dye	743:761	The synthetic composites were applied in photocatalytic removal of malachite green dye under a visible light source.
31929075	3	29	from	reduction	522:530	arg1	bandgap					539:545	the bandgap	535:545	the bandgap of ZnO from 3.3 eV to 2.85 eV and 2.5 eV	535:586	Additionally, the optical studies reflected a reduction in the bandgap of ZnO from 3.3 eV to 2.85 eV and 2.5 eV after supporting it onto chitosan chains and after doping it with cerium, respectively.
31929075	5	30	theme	pure	933:936	arg1	ZnO					938:940	the pure ZnO	929:940	the pure ZnO	929:940	The synthetic CH/ZnO and CH/Ce-ZnO showed enhancement in the photocatalytic removal of M.G by 54% and 87%, respectively, as compared to the pure ZnO.
31929075	2	31	theme	Ce	422:423	arg1	ZnO					431:433	ZnO and Ce doped ZnO	414:433	ZnO and Ce doped ZnO at stunning micro flowers of nano limps	414:473	The morphological investigation reflected the formation of ZnO and Ce doped ZnO at stunning micro flowers of nano limps.
31929075	0	32	theme	malachite	30:38	arg1	green					40:44	malachite green dye	30:48	malachite green dye using chitosan	30:63	Photocatalytic degradation of malachite green dye using chitosan supported ZnO and Ce-ZnO nano-flowers under visible light.
31929075	1	33	theme	activity	321:328	arg1	types					128:132	Two types	124:132	Two types of chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites)	124:207	Two types of chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites) were synthesized under microwave irradiation and characterized as advanced catalysts of enhanced photocatalytic activity under the visible light.
31929075	1	33	theme	activity	321:328	arg1	catalysts					284:292	advanced catalysts	275:292	advanced catalysts of enhanced photocatalytic activity under the visible light	275:352	Two types of chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites) were synthesized under microwave irradiation and characterized as advanced catalysts of enhanced photocatalytic activity under the visible light.
31929075	8	34	theme	hydroxyl	1300:1307	arg1	species					1234:1240	The effective species	1220:1240	The effective species in CH/Ce-ZnO photocatalytic system	1220:1275	The effective species in CH/Ce-ZnO photocatalytic system are the photogenerated hydroxyl radicals followed by the electron-hole pairs.
31929075	8	34	theme	hydroxyl	1300:1307	arg1	radicals					1309:1316	the photogenerated hydroxyl radicals	1281:1316	the photogenerated hydroxyl radicals followed by the electron-hole pairs	1281:1352	The effective species in CH/Ce-ZnO photocatalytic system are the photogenerated hydroxyl radicals followed by the electron-hole pairs.
31929075	4	35	theme	visible	771:777	arg1	source					785:790	a visible light source	769:790	a visible light source	769:790	The synthetic composites were applied in photocatalytic removal of malachite green dye under a visible light source.
31929075	6	36	theme	removal	1063:1069	arg1	percentages					1071:1081	stunning removal percentages	1054:1081	stunning removal percentages	1054:1081	The synthetic composites are of high stability and can be reused for five photocatalytic degradation cycles at stunning removal percentages.
31929075	3	37	theme	optical	494:500	arg1	studies					502:508	the optical studies	490:508	the optical studies	490:508	Additionally, the optical studies reflected a reduction in the bandgap of ZnO from 3.3 eV to 2.85 eV and 2.5 eV after supporting it onto chitosan chains and after doping it with cerium, respectively.
31929075	2	38	theme	nano	464:467	arg1	limps					469:473	nano limps	464:473	nano limps	464:473	The morphological investigation reflected the formation of ZnO and Ce doped ZnO at stunning micro flowers of nano limps.
31929075	6	39	theme	synthetic	947:955	arg1	composites					957:966	The synthetic composites	943:966	The synthetic composites	943:966	The synthetic composites are of high stability and can be reused for five photocatalytic degradation cycles at stunning removal percentages.
31929075	6	40	theme	degradation	1032:1042	arg1	cycles					1044:1049	five photocatalytic degradation cycles	1012:1049	five photocatalytic degradation cycles	1012:1049	The synthetic composites are of high stability and can be reused for five photocatalytic degradation cycles at stunning removal percentages.
31929075	5	41	theme	synthetic	797:805	arg1	CH/ZnO					807:812	The synthetic CH/ZnO	793:812	The synthetic CH/ZnO	793:812	The synthetic CH/ZnO and CH/Ce-ZnO showed enhancement in the photocatalytic removal of M.G by 54% and 87%, respectively, as compared to the pure ZnO.
31929075	6	42	theme	photocatalytic	1017:1030	arg1	cycles					1044:1049	five photocatalytic degradation cycles	1012:1049	five photocatalytic degradation cycles	1012:1049	The synthetic composites are of high stability and can be reused for five photocatalytic degradation cycles at stunning removal percentages.
31929075	3	43	theme	chitosan	613:620	arg1	chains					622:627	chitosan chains	613:627	chitosan chains	613:627	Additionally, the optical studies reflected a reduction in the bandgap of ZnO from 3.3 eV to 2.85 eV and 2.5 eV after supporting it onto chitosan chains and after doping it with cerium, respectively.
31929075	4	44	theme	synthetic	680:688	arg1	composites					690:699	The synthetic composites	676:699	The synthetic composites	676:699	The synthetic composites were applied in photocatalytic removal of malachite green dye under a visible light source.
31929075	2	45	theme	micro	447:451	arg1	flowers					453:459	stunning micro flowers	438:459	stunning micro flowers of nano limps	438:473	The morphological investigation reflected the formation of ZnO and Ce doped ZnO at stunning micro flowers of nano limps.
31929075	0	46	theme	green	40:44	arg1	degradation					15:25	Photocatalytic degradation	0:25	Photocatalytic degradation of malachite green dye using chitosan	0:63	Photocatalytic degradation of malachite green dye using chitosan supported ZnO and Ce-ZnO nano-flowers under visible light.
31929075	1	47	theme	visible	340:346	arg1	light					348:352	the visible light	336:352	the visible light	336:352	Two types of chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites) were synthesized under microwave irradiation and characterized as advanced catalysts of enhanced photocatalytic activity under the visible light.
31929075	2	48	theme	limps	469:473	arg1	flowers					453:459	stunning micro flowers	438:459	stunning micro flowers of nano limps	438:473	The morphological investigation reflected the formation of ZnO and Ce doped ZnO at stunning micro flowers of nano limps.
31929075	5	49	theme	M.G	880:882	arg1	removal					869:875	the photocatalytic removal	850:875	the photocatalytic removal of M.G by 54% and 87%, respectively	850:911	The synthetic CH/ZnO and CH/Ce-ZnO showed enhancement in the photocatalytic removal of M.G by 54% and 87%, respectively, as compared to the pure ZnO.
31929075	1	50	theme	microwave	232:240	arg1	irradiation					242:252	microwave irradiation	232:252	microwave irradiation	232:252	Two types of chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites) were synthesized under microwave irradiation and characterized as advanced catalysts of enhanced photocatalytic activity under the visible light.
31929075	8	51	from	species	1234:1240	arg1	system					1270:1275	CH/Ce-ZnO photocatalytic system	1245:1275	CH/Ce-ZnO photocatalytic system	1245:1275	The effective species in CH/Ce-ZnO photocatalytic system are the photogenerated hydroxyl radicals followed by the electron-hole pairs.
31929075	8	52	theme	photogenerated	1285:1298	arg1	species					1234:1240	The effective species	1220:1240	The effective species in CH/Ce-ZnO photocatalytic system	1220:1275	The effective species in CH/Ce-ZnO photocatalytic system are the photogenerated hydroxyl radicals followed by the electron-hole pairs.
31929075	8	52	theme	photogenerated	1285:1298	arg1	radicals					1309:1316	the photogenerated hydroxyl radicals	1281:1316	the photogenerated hydroxyl radicals followed by the electron-hole pairs	1281:1352	The effective species in CH/Ce-ZnO photocatalytic system are the photogenerated hydroxyl radicals followed by the electron-hole pairs.
31929075	7	53	theme	M.G	1134:1136	arg1	removal					1123:1129	the removal	1119:1129	the removal of M.G by CH/ZnO	1119:1146	The main oxidizing radicals during the removal of M.G by CH/ZnO are the generated electron-hole pairs as well as the hydroxyl radicals.
31929075	4	54	theme	green	753:757	arg1	removal					732:738	photocatalytic removal	717:738	photocatalytic removal of malachite green dye	717:761	The synthetic composites were applied in photocatalytic removal of malachite green dye under a visible light source.
31929075	1	55	theme	composites	152:161	arg1	types					128:132	Two types	124:132	Two types of chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites)	124:207	Two types of chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites) were synthesized under microwave irradiation and characterized as advanced catalysts of enhanced photocatalytic activity under the visible light.
31929075	1	55	theme	composites	152:161	arg1	catalysts					284:292	advanced catalysts	275:292	advanced catalysts of enhanced photocatalytic activity under the visible light	275:352	Two types of chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites) were synthesized under microwave irradiation and characterized as advanced catalysts of enhanced photocatalytic activity under the visible light.
31929075	2	56	theme	stunning	438:445	arg1	flowers					453:459	stunning micro flowers	438:459	stunning micro flowers of nano limps	438:473	The morphological investigation reflected the formation of ZnO and Ce doped ZnO at stunning micro flowers of nano limps.
31929075	5	57	theme	photocatalytic	854:867	arg1	removal					869:875	the photocatalytic removal	850:875	the photocatalytic removal of M.G by 54% and 87%, respectively	850:911	The synthetic CH/ZnO and CH/Ce-ZnO showed enhancement in the photocatalytic removal of M.G by 54% and 87%, respectively, as compared to the pure ZnO.
31929075	1	58	theme	chitosan-based	137:150	arg1	composites					197:206	chitosan/ZnO and chitosan/Ce-ZnO composites	164:206	chitosan/ZnO and chitosan/Ce-ZnO composites	164:206	Two types of chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites) were synthesized under microwave irradiation and characterized as advanced catalysts of enhanced photocatalytic activity under the visible light.
31929075	1	58	theme	chitosan-based	137:150	arg1	composites					152:161	chitosan-based composites	137:161	chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites)	137:207	Two types of chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites) were synthesized under microwave irradiation and characterized as advanced catalysts of enhanced photocatalytic activity under the visible light.
31929075	1	59	theme	chitosan/Ce-ZnO	181:195	arg1	composites					197:206	chitosan/ZnO and chitosan/Ce-ZnO composites	164:206	chitosan/ZnO and chitosan/Ce-ZnO composites	164:206	Two types of chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites) were synthesized under microwave irradiation and characterized as advanced catalysts of enhanced photocatalytic activity under the visible light.
31929075	1	59	theme	chitosan/Ce-ZnO	181:195	arg1	composites					152:161	chitosan-based composites	137:161	chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites)	137:207	Two types of chitosan-based composites (chitosan/ZnO and chitosan/Ce-ZnO composites) were synthesized under microwave irradiation and characterized as advanced catalysts of enhanced photocatalytic activity under the visible light.
31929075	2	60	theme	ZnO	414:416	arg1	ZnO					431:433	ZnO and Ce doped ZnO	414:433	ZnO and Ce doped ZnO at stunning micro flowers of nano limps	414:473	The morphological investigation reflected the formation of ZnO and Ce doped ZnO at stunning micro flowers of nano limps.
31929075	7	61	theme	electron-hole	1166:1178	arg1	pairs					1180:1184	the generated electron-hole pairs	1152:1184	the generated electron-hole pairs as well as the hydroxyl radicals	1152:1217	The main oxidizing radicals during the removal of M.G by CH/ZnO are the generated electron-hole pairs as well as the hydroxyl radicals.
31929075	7	61	theme	electron-hole	1166:1178	arg1	radicals					1103:1110	The main oxidizing radicals	1084:1110	The main oxidizing radicals during the removal of M.G by CH/ZnO	1084:1146	The main oxidizing radicals during the removal of M.G by CH/ZnO are the generated electron-hole pairs as well as the hydroxyl radicals.
33499020	4	0	theme	blood	842:846	arg1	loss					848:851	blood loss	842:851	blood loss	842:851	By combining more than one hemostatic agent (i.e., chitosan and kaolin) that act via more than one mechanism, and by utilizing different nanotechnology-based approaches to enhance the surface areas, the capability of the dressing to control bleeding was improved, in terms of amount of blood loss and time to hemostasis.
33499020	6	1	theme	Combat	1300:1305	arg1	Gauze					1307:1311	QuikClot® Combat Gauze	1290:1311	QuikClot® Combat Gauze	1290:1311	The developed composites effectively controlled bleeding and significantly improved hemostasis and survival rates in two animal models, rats and rabbits, compared to conventional dressings and QuikClot® Combat Gauze.
33499020	6	2	from	rates	1205:1209	arg1	rabbits					1242:1248	rabbits	1242:1248	rabbits	1242:1248	The developed composites effectively controlled bleeding and significantly improved hemostasis and survival rates in two animal models, rats and rabbits, compared to conventional dressings and QuikClot® Combat Gauze.
33499020	6	2	from	rates	1205:1209	arg1	rats					1233:1236	rats	1233:1236	rats	1233:1236	The developed composites effectively controlled bleeding and significantly improved hemostasis and survival rates in two animal models, rats and rabbits, compared to conventional dressings and QuikClot® Combat Gauze.
33499020	6	2	from	rates	1205:1209	arg1	models					1225:1230	two animal models	1214:1230	two animal models	1214:1230	The developed composites effectively controlled bleeding and significantly improved hemostasis and survival rates in two animal models, rats and rabbits, compared to conventional dressings and QuikClot® Combat Gauze.
33499020	5	3	theme	nanotechnology-based	881:900	arg1	approaches					902:911	The nanotechnology-based approaches	877:911	The nanotechnology-based approaches utilized to enhance the effective surface area of the hemostatic agents	877:983	The nanotechnology-based approaches utilized to enhance the effective surface area of the hemostatic agents included the use of Pluronic nanoparticles, and deposition of chitosan micro- and nano-fibers onto the carrier.
33499020	1	4	theme	chitosan	178:185	arg1	combination					163:173	a combination	161:173	a combination of chitosan and kaolin	161:196	In the current study, hemostatic compositions including a combination of chitosan and kaolin have been developed.
33499020	2	5	attach	derived	255:261	arg1	chitins					268:274	chitins	268:274	chitins	268:274	Chitosan is a marine polysaccharide derived from chitins, a structural component in the shells of crustaceans.
33499020	2	5	attach	derived	255:261	arg2	Chitosan					219:226	Chitosan	219:226	Chitosan	219:226	Chitosan is a marine polysaccharide derived from chitins, a structural component in the shells of crustaceans.
33499020	2	5	attach	derived	255:261	arg1	component					290:298	a structural component	277:298	a structural component in the shells of crustaceans	277:327	Chitosan is a marine polysaccharide derived from chitins, a structural component in the shells of crustaceans.
33499020	2	5	attach	derived	255:261	arg2	polysaccharide					240:253	a marine polysaccharide	231:253	a marine polysaccharide derived from chitins, a structural component in the shells of crustaceans	231:327	Chitosan is a marine polysaccharide derived from chitins, a structural component in the shells of crustaceans.
33499020	7	6	theme	dressings	1498:1506	arg1	application					1479:1489	application	1479:1489	application of the dressings	1479:1506	The composites were well-tolerated as demonstrated by their in vivo biocompatibility and absence of clinical and biochemical changes in the laboratory animals after application of the dressings.
33499020	5	7	theme	Pluronic	1005:1012	arg1	nanoparticles					1014:1026	Pluronic nanoparticles	1005:1026	Pluronic nanoparticles	1005:1026	The nanotechnology-based approaches utilized to enhance the effective surface area of the hemostatic agents included the use of Pluronic nanoparticles, and deposition of chitosan micro- and nano-fibers onto the carrier.
33499020	5	8	theme	surface	947:953	arg1	area					955:958	the effective surface area	933:958	the effective surface area of the hemostatic agents	933:983	The nanotechnology-based approaches utilized to enhance the effective surface area of the hemostatic agents included the use of Pluronic nanoparticles, and deposition of chitosan micro- and nano-fibers onto the carrier.
33499020	5	9	theme	effective	937:945	arg1	area					955:958	the effective surface area	933:958	the effective surface area of the hemostatic agents	933:983	The nanotechnology-based approaches utilized to enhance the effective surface area of the hemostatic agents included the use of Pluronic nanoparticles, and deposition of chitosan micro- and nano-fibers onto the carrier.
33499020	4	10	theme	loss	848:851	arg1	time					857:860	time	857:860	time to hemostasis	857:874	By combining more than one hemostatic agent (i.e., chitosan and kaolin) that act via more than one mechanism, and by utilizing different nanotechnology-based approaches to enhance the surface areas, the capability of the dressing to control bleeding was improved, in terms of amount of blood loss and time to hemostasis.
33499020	4	10	theme	loss	848:851	arg1	amount					832:837	amount	832:837	amount of blood loss	832:851	By combining more than one hemostatic agent (i.e., chitosan and kaolin) that act via more than one mechanism, and by utilizing different nanotechnology-based approaches to enhance the surface areas, the capability of the dressing to control bleeding was improved, in terms of amount of blood loss and time to hemostasis.
33499020	4	10	theme	loss	848:851	arg1	loss					848:851	blood loss	842:851	blood loss	842:851	By combining more than one hemostatic agent (i.e., chitosan and kaolin) that act via more than one mechanism, and by utilizing different nanotechnology-based approaches to enhance the surface areas, the capability of the dressing to control bleeding was improved, in terms of amount of blood loss and time to hemostasis.
33499020	6	11	theme	conventional	1263:1274	arg1	dressings					1276:1284	conventional dressings	1263:1284	conventional dressings	1263:1284	The developed composites effectively controlled bleeding and significantly improved hemostasis and survival rates in two animal models, rats and rabbits, compared to conventional dressings and QuikClot® Combat Gauze.
33499020	7	12	theme	laboratory	1454:1463	arg1	animals					1465:1471	the laboratory animals	1450:1471	the laboratory animals	1450:1471	The composites were well-tolerated as demonstrated by their in vivo biocompatibility and absence of clinical and biochemical changes in the laboratory animals after application of the dressings.
33499020	6	13	from	hemostasis	1181:1190	arg1	rabbits					1242:1248	rabbits	1242:1248	rabbits	1242:1248	The developed composites effectively controlled bleeding and significantly improved hemostasis and survival rates in two animal models, rats and rabbits, compared to conventional dressings and QuikClot® Combat Gauze.
33499020	6	13	from	hemostasis	1181:1190	arg1	rats					1233:1236	rats	1233:1236	rats	1233:1236	The developed composites effectively controlled bleeding and significantly improved hemostasis and survival rates in two animal models, rats and rabbits, compared to conventional dressings and QuikClot® Combat Gauze.
33499020	6	13	from	hemostasis	1181:1190	arg1	models					1225:1230	two animal models	1214:1230	two animal models	1214:1230	The developed composites effectively controlled bleeding and significantly improved hemostasis and survival rates in two animal models, rats and rabbits, compared to conventional dressings and QuikClot® Combat Gauze.
33499020	3	14	theme	hemostatic	533:542	arg1	composites					544:553	hemostatic composites	533:553	hemostatic composites	533:553	Both chitosan and kaolin have the ability to mediate a quick and efficient hemostatic effect following immediate application to injury sites, and thus they have been widely exploited in manufacturing of hemostatic composites.
33499020	0	15	theme	Nanocomposites	53:66	arg1	Design					0:5	Design	0:5	Design	0:5	Design and Preclinical Evaluation of Chitosan/Kaolin Nanocomposites with Enhanced Hemostatic Efficiency.
33499020	0	15	theme	Nanocomposites	53:66	arg1	Evaluation					23:32	Preclinical Evaluation	11:32	Preclinical Evaluation	11:32	Design and Preclinical Evaluation of Chitosan/Kaolin Nanocomposites with Enhanced Hemostatic Efficiency.
33499020	2	16	theme	structural	279:288	arg1	chitins					268:274	chitins	268:274	chitins	268:274	Chitosan is a marine polysaccharide derived from chitins, a structural component in the shells of crustaceans.
33499020	2	16	theme	structural	279:288	arg1	component					290:298	a structural component	277:298	a structural component in the shells of crustaceans	277:327	Chitosan is a marine polysaccharide derived from chitins, a structural component in the shells of crustaceans.
33499020	1	17	theme	kaolin	191:196	arg1	combination					163:173	a combination	161:173	a combination of chitosan and kaolin	161:196	In the current study, hemostatic compositions including a combination of chitosan and kaolin have been developed.
33499020	3	18	theme	quick	385:389	arg1	effect					416:421	a quick and efficient hemostatic effect	383:421	a quick and efficient hemostatic effect	383:421	Both chitosan and kaolin have the ability to mediate a quick and efficient hemostatic effect following immediate application to injury sites, and thus they have been widely exploited in manufacturing of hemostatic composites.
33499020	4	19	theme	amount	832:837	arg1	terms					823:827	terms	823:827	terms of amount of blood loss and time to hemostasis	823:874	By combining more than one hemostatic agent (i.e., chitosan and kaolin) that act via more than one mechanism, and by utilizing different nanotechnology-based approaches to enhance the surface areas, the capability of the dressing to control bleeding was improved, in terms of amount of blood loss and time to hemostasis.
33499020	3	20	theme	immediate	433:441	arg1	application					443:453	immediate application	433:453	immediate application to injury sites	433:469	Both chitosan and kaolin have the ability to mediate a quick and efficient hemostatic effect following immediate application to injury sites, and thus they have been widely exploited in manufacturing of hemostatic composites.
33499020	2	21	theme	crustaceans	317:327	arg1	shells					307:312	the shells	303:312	the shells of crustaceans	303:327	Chitosan is a marine polysaccharide derived from chitins, a structural component in the shells of crustaceans.
33499020	7	22	theme	biochemical	1427:1437	arg1	changes					1439:1445	clinical and biochemical changes	1414:1445	clinical and biochemical changes in the laboratory animals after application of the dressings	1414:1506	The composites were well-tolerated as demonstrated by their in vivo biocompatibility and absence of clinical and biochemical changes in the laboratory animals after application of the dressings.
33499020	1	23	theme	current	112:118	arg1	study					120:124	the current study	108:124	the current study	108:124	In the current study, hemostatic compositions including a combination of chitosan and kaolin have been developed.
33499020	7	24	from	biocompatibility	1382:1397	arg1	animals					1465:1471	the laboratory animals	1450:1471	the laboratory animals	1450:1471	The composites were well-tolerated as demonstrated by their in vivo biocompatibility and absence of clinical and biochemical changes in the laboratory animals after application of the dressings.
33499020	0	25	theme	Preclinical	11:21	arg1	Evaluation					23:32	Preclinical Evaluation	11:32	Preclinical Evaluation	11:32	Design and Preclinical Evaluation of Chitosan/Kaolin Nanocomposites with Enhanced Hemostatic Efficiency.
33499020	6	26	theme	survival	1196:1203	arg1	rates					1205:1209	survival rates	1196:1209	survival rates	1196:1209	The developed composites effectively controlled bleeding and significantly improved hemostasis and survival rates in two animal models, rats and rabbits, compared to conventional dressings and QuikClot® Combat Gauze.
33499020	7	27	theme	changes	1439:1445	arg1	biocompatibility					1382:1397	their in vivo biocompatibility	1368:1397	their in vivo biocompatibility	1368:1397	The composites were well-tolerated as demonstrated by their in vivo biocompatibility and absence of clinical and biochemical changes in the laboratory animals after application of the dressings.
33499020	7	27	theme	changes	1439:1445	arg1	absence					1403:1409	absence	1403:1409	absence	1403:1409	The composites were well-tolerated as demonstrated by their in vivo biocompatibility and absence of clinical and biochemical changes in the laboratory animals after application of the dressings.
33499020	5	28	theme	chitosan	1047:1054	arg1	micro-					1056:1061	chitosan micro-	1047:1061	chitosan micro-	1047:1061	The nanotechnology-based approaches utilized to enhance the effective surface area of the hemostatic agents included the use of Pluronic nanoparticles, and deposition of chitosan micro- and nano-fibers onto the carrier.
33499020	4	29	theme	surface	740:746	arg1	areas					748:752	the surface areas	736:752	the surface areas	736:752	By combining more than one hemostatic agent (i.e., chitosan and kaolin) that act via more than one mechanism, and by utilizing different nanotechnology-based approaches to enhance the surface areas, the capability of the dressing to control bleeding was improved, in terms of amount of blood loss and time to hemostasis.
33499020	7	30	theme	in	1374:1375	arg1	biocompatibility					1382:1397	their in vivo biocompatibility	1368:1397	their in vivo biocompatibility	1368:1397	The composites were well-tolerated as demonstrated by their in vivo biocompatibility and absence of clinical and biochemical changes in the laboratory animals after application of the dressings.
33499020	4	31	theme	different	683:691	arg1	approaches					714:723	different nanotechnology-based approaches	683:723	different nanotechnology-based approaches	683:723	By combining more than one hemostatic agent (i.e., chitosan and kaolin) that act via more than one mechanism, and by utilizing different nanotechnology-based approaches to enhance the surface areas, the capability of the dressing to control bleeding was improved, in terms of amount of blood loss and time to hemostasis.
33499020	5	32	theme	micro-	1056:1061	arg1	deposition					1033:1042	deposition	1033:1042	deposition of chitosan micro- and nano-fibers onto the carrier	1033:1094	The nanotechnology-based approaches utilized to enhance the effective surface area of the hemostatic agents included the use of Pluronic nanoparticles, and deposition of chitosan micro- and nano-fibers onto the carrier.
33499020	5	32	theme	micro-	1056:1061	arg1	use					998:1000	the use	994:1000	the use of Pluronic nanoparticles	994:1026	The nanotechnology-based approaches utilized to enhance the effective surface area of the hemostatic agents included the use of Pluronic nanoparticles, and deposition of chitosan micro- and nano-fibers onto the carrier.
33499020	6	33	theme	QuikClot®	1290:1298	arg1	Gauze					1307:1311	QuikClot® Combat Gauze	1290:1311	QuikClot® Combat Gauze	1290:1311	The developed composites effectively controlled bleeding and significantly improved hemostasis and survival rates in two animal models, rats and rabbits, compared to conventional dressings and QuikClot® Combat Gauze.
33499020	7	34	from	absence	1403:1409	arg1	animals					1465:1471	the laboratory animals	1450:1471	the laboratory animals	1450:1471	The composites were well-tolerated as demonstrated by their in vivo biocompatibility and absence of clinical and biochemical changes in the laboratory animals after application of the dressings.
33499020	4	35	theme	hemostatic	583:592	arg1	agent					594:598	more than one hemostatic agent	569:598	more than one hemostatic agent (i.e., chitosan and kaolin) that act via more than one mechanism, and by utilizing different nanotechnology-based approaches to enhance the surface areas	569:752	By combining more than one hemostatic agent (i.e., chitosan and kaolin) that act via more than one mechanism, and by utilizing different nanotechnology-based approaches to enhance the surface areas, the capability of the dressing to control bleeding was improved, in terms of amount of blood loss and time to hemostasis.
33499020	0	36	with	Evaluation	23:32	arg1	Efficiency					93:102	Enhanced Hemostatic Efficiency	73:102	Enhanced Hemostatic Efficiency	73:102	Design and Preclinical Evaluation of Chitosan/Kaolin Nanocomposites with Enhanced Hemostatic Efficiency.
33499020	7	37	dep	in	1374:1375	arg1	vivo					1377:1380	vivo	1377:1380	vivo	1377:1380	The composites were well-tolerated as demonstrated by their in vivo biocompatibility and absence of clinical and biochemical changes in the laboratory animals after application of the dressings.
33499020	3	38	theme	efficient	395:403	arg1	effect					416:421	a quick and efficient hemostatic effect	383:421	a quick and efficient hemostatic effect	383:421	Both chitosan and kaolin have the ability to mediate a quick and efficient hemostatic effect following immediate application to injury sites, and thus they have been widely exploited in manufacturing of hemostatic composites.
33499020	5	39	theme	nano-fibers	1067:1077	arg1	deposition					1033:1042	deposition	1033:1042	deposition of chitosan micro- and nano-fibers onto the carrier	1033:1094	The nanotechnology-based approaches utilized to enhance the effective surface area of the hemostatic agents included the use of Pluronic nanoparticles, and deposition of chitosan micro- and nano-fibers onto the carrier.
33499020	5	39	theme	nano-fibers	1067:1077	arg1	use					998:1000	the use	994:1000	the use of Pluronic nanoparticles	994:1026	The nanotechnology-based approaches utilized to enhance the effective surface area of the hemostatic agents included the use of Pluronic nanoparticles, and deposition of chitosan micro- and nano-fibers onto the carrier.
33499020	2	40	theme	marine	233:238	arg1	polysaccharide					240:253	a marine polysaccharide	231:253	a marine polysaccharide derived from chitins, a structural component in the shells of crustaceans	231:327	Chitosan is a marine polysaccharide derived from chitins, a structural component in the shells of crustaceans.
33499020	2	40	theme	marine	233:238	arg1	Chitosan					219:226	Chitosan	219:226	Chitosan	219:226	Chitosan is a marine polysaccharide derived from chitins, a structural component in the shells of crustaceans.
33499020	3	41	contain	have	355:358	arg2	ability					364:370	the ability to mediate a quick and efficient hemostatic effect following immediate application to injury sites	360:469	the ability to mediate a quick and efficient hemostatic effect following immediate application to injury sites	360:469	Both chitosan and kaolin have the ability to mediate a quick and efficient hemostatic effect following immediate application to injury sites, and thus they have been widely exploited in manufacturing of hemostatic composites.
33499020	3	41	contain	have	355:358	arg1	kaolin					348:353	kaolin	348:353	kaolin	348:353	Both chitosan and kaolin have the ability to mediate a quick and efficient hemostatic effect following immediate application to injury sites, and thus they have been widely exploited in manufacturing of hemostatic composites.
33499020	3	41	contain	have	355:358	arg1	chitosan					335:342	chitosan	335:342	chitosan	335:342	Both chitosan and kaolin have the ability to mediate a quick and efficient hemostatic effect following immediate application to injury sites, and thus they have been widely exploited in manufacturing of hemostatic composites.
33499020	4	42	theme	nanotechnology-based	693:712	arg1	approaches					714:723	different nanotechnology-based approaches	683:723	different nanotechnology-based approaches	683:723	By combining more than one hemostatic agent (i.e., chitosan and kaolin) that act via more than one mechanism, and by utilizing different nanotechnology-based approaches to enhance the surface areas, the capability of the dressing to control bleeding was improved, in terms of amount of blood loss and time to hemostasis.
33499020	3	43	theme	hemostatic	405:414	arg1	effect					416:421	a quick and efficient hemostatic effect	383:421	a quick and efficient hemostatic effect	383:421	Both chitosan and kaolin have the ability to mediate a quick and efficient hemostatic effect following immediate application to injury sites, and thus they have been widely exploited in manufacturing of hemostatic composites.
33499020	4	44	theme	time	857:860	arg1	terms					823:827	terms	823:827	terms of amount of blood loss and time to hemostasis	823:874	By combining more than one hemostatic agent (i.e., chitosan and kaolin) that act via more than one mechanism, and by utilizing different nanotechnology-based approaches to enhance the surface areas, the capability of the dressing to control bleeding was improved, in terms of amount of blood loss and time to hemostasis.
33499020	5	45	theme	nanoparticles	1014:1026	arg1	deposition					1033:1042	deposition	1033:1042	deposition of chitosan micro- and nano-fibers onto the carrier	1033:1094	The nanotechnology-based approaches utilized to enhance the effective surface area of the hemostatic agents included the use of Pluronic nanoparticles, and deposition of chitosan micro- and nano-fibers onto the carrier.
33499020	5	45	theme	nanoparticles	1014:1026	arg1	use					998:1000	the use	994:1000	the use of Pluronic nanoparticles	994:1026	The nanotechnology-based approaches utilized to enhance the effective surface area of the hemostatic agents included the use of Pluronic nanoparticles, and deposition of chitosan micro- and nano-fibers onto the carrier.
33499020	1	46	theme	hemostatic	127:136	arg1	compositions					138:149	hemostatic compositions	127:149	hemostatic compositions including a combination of chitosan and kaolin	127:196	In the current study, hemostatic compositions including a combination of chitosan and kaolin have been developed.
33499020	1	46	theme	hemostatic	127:136	arg1	combination					163:173	a combination	161:173	a combination of chitosan and kaolin	161:196	In the current study, hemostatic compositions including a combination of chitosan and kaolin have been developed.
33499020	2	47	from	component	290:298	arg1	shells					307:312	the shells	303:312	the shells of crustaceans	303:327	Chitosan is a marine polysaccharide derived from chitins, a structural component in the shells of crustaceans.
33499020	7	48	from	animals	1465:1471	arg1	biocompatibility					1382:1397	their in vivo biocompatibility	1368:1397	their in vivo biocompatibility	1368:1397	The composites were well-tolerated as demonstrated by their in vivo biocompatibility and absence of clinical and biochemical changes in the laboratory animals after application of the dressings.
33499020	7	48	from	animals	1465:1471	arg1	absence					1403:1409	absence	1403:1409	absence	1403:1409	The composites were well-tolerated as demonstrated by their in vivo biocompatibility and absence of clinical and biochemical changes in the laboratory animals after application of the dressings.
33499020	5	49	theme	hemostatic	967:976	arg1	agents					978:983	the hemostatic agents	963:983	the hemostatic agents	963:983	The nanotechnology-based approaches utilized to enhance the effective surface area of the hemostatic agents included the use of Pluronic nanoparticles, and deposition of chitosan micro- and nano-fibers onto the carrier.
33499020	4	50	theme	dressing	777:784	arg1	capability					759:768	the capability	755:768	the capability of the dressing to control bleeding	755:804	By combining more than one hemostatic agent (i.e., chitosan and kaolin) that act via more than one mechanism, and by utilizing different nanotechnology-based approaches to enhance the surface areas, the capability of the dressing to control bleeding was improved, in terms of amount of blood loss and time to hemostasis.
33499020	0	51	with	Design	0:5	arg1	Efficiency					93:102	Enhanced Hemostatic Efficiency	73:102	Enhanced Hemostatic Efficiency	73:102	Design and Preclinical Evaluation of Chitosan/Kaolin Nanocomposites with Enhanced Hemostatic Efficiency.
33499020	6	52	theme	developed	1101:1109	arg1	composites					1111:1120	The developed composites	1097:1120	The developed composites	1097:1120	The developed composites effectively controlled bleeding and significantly improved hemostasis and survival rates in two animal models, rats and rabbits, compared to conventional dressings and QuikClot® Combat Gauze.
33499020	6	53	theme	bleeding	1145:1152	arg1	hemostasis					1181:1190	bleeding and significantly improved hemostasis	1145:1190	bleeding and significantly improved hemostasis	1145:1190	The developed composites effectively controlled bleeding and significantly improved hemostasis and survival rates in two animal models, rats and rabbits, compared to conventional dressings and QuikClot® Combat Gauze.
33499020	7	54	theme	clinical	1414:1421	arg1	changes					1439:1445	clinical and biochemical changes	1414:1445	clinical and biochemical changes in the laboratory animals after application of the dressings	1414:1506	The composites were well-tolerated as demonstrated by their in vivo biocompatibility and absence of clinical and biochemical changes in the laboratory animals after application of the dressings.
33499020	3	55	theme	composites	544:553	arg1	manufacturing					516:528	manufacturing	516:528	manufacturing of hemostatic composites	516:553	Both chitosan and kaolin have the ability to mediate a quick and efficient hemostatic effect following immediate application to injury sites, and thus they have been widely exploited in manufacturing of hemostatic composites.
33499020	4	56	dep	chitosan	607:614	arg1	i.e.					601:604	i.e.	601:604	i.e.	601:604	By combining more than one hemostatic agent (i.e., chitosan and kaolin) that act via more than one mechanism, and by utilizing different nanotechnology-based approaches to enhance the surface areas, the capability of the dressing to control bleeding was improved, in terms of amount of blood loss and time to hemostasis.
33499020	5	57	theme	agents	978:983	arg1	area					955:958	the effective surface area	933:958	the effective surface area of the hemostatic agents	933:983	The nanotechnology-based approaches utilized to enhance the effective surface area of the hemostatic agents included the use of Pluronic nanoparticles, and deposition of chitosan micro- and nano-fibers onto the carrier.
33499020	0	58	theme	Hemostatic	82:91	arg1	Efficiency					93:102	Enhanced Hemostatic Efficiency	73:102	Enhanced Hemostatic Efficiency	73:102	Design and Preclinical Evaluation of Chitosan/Kaolin Nanocomposites with Enhanced Hemostatic Efficiency.
33499020	6	59	theme	improved	1172:1179	arg1	hemostasis					1181:1190	bleeding and significantly improved hemostasis	1145:1190	bleeding and significantly improved hemostasis	1145:1190	The developed composites effectively controlled bleeding and significantly improved hemostasis and survival rates in two animal models, rats and rabbits, compared to conventional dressings and QuikClot® Combat Gauze.
33499020	3	60	theme	injury	458:463	arg1	sites					465:469	injury sites	458:469	injury sites	458:469	Both chitosan and kaolin have the ability to mediate a quick and efficient hemostatic effect following immediate application to injury sites, and thus they have been widely exploited in manufacturing of hemostatic composites.
33499020	0	61	theme	Enhanced	73:80	arg1	Efficiency					93:102	Enhanced Hemostatic Efficiency	73:102	Enhanced Hemostatic Efficiency	73:102	Design and Preclinical Evaluation of Chitosan/Kaolin Nanocomposites with Enhanced Hemostatic Efficiency.
33499020	6	62	theme	animal	1218:1223	arg1	rabbits					1242:1248	rabbits	1242:1248	rabbits	1242:1248	The developed composites effectively controlled bleeding and significantly improved hemostasis and survival rates in two animal models, rats and rabbits, compared to conventional dressings and QuikClot® Combat Gauze.
33499020	6	62	theme	animal	1218:1223	arg1	rats					1233:1236	rats	1233:1236	rats	1233:1236	The developed composites effectively controlled bleeding and significantly improved hemostasis and survival rates in two animal models, rats and rabbits, compared to conventional dressings and QuikClot® Combat Gauze.
33499020	6	62	theme	animal	1218:1223	arg1	models					1225:1230	two animal models	1214:1230	two animal models	1214:1230	The developed composites effectively controlled bleeding and significantly improved hemostasis and survival rates in two animal models, rats and rabbits, compared to conventional dressings and QuikClot® Combat Gauze.
33499020	7	63	from	changes	1439:1445	arg1	animals					1465:1471	the laboratory animals	1450:1471	the laboratory animals	1450:1471	The composites were well-tolerated as demonstrated by their in vivo biocompatibility and absence of clinical and biochemical changes in the laboratory animals after application of the dressings.
34689631	3	0	theme	three-dimensional	465:481	arg1	film					488:491	porous three-dimensional (3D) film	458:491	porous three-dimensional (3D) film	458:491	To overcome these issues, a novel type of porous three-dimensional (3D) film was fabricated using chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC) loaded with 45S5 bioglass (CCMPBG) by the freeze-drying method for wound healing application.
34689631	7	1	theme	BG	1382:1383	arg1	effects					1328:1334	the synergistic effects	1312:1334	the synergistic effects of chitosan, carboxymethyl pullulan (CMP) and BG	1312:1383	Furthermore, CCMPBG films presented the improved biocompatibility, antimicrobial activity and wound closure ability because of the synergistic effects of chitosan, carboxymethyl pullulan (CMP) and BG.
34689631	3	2	theme	healing	649:655	arg1	application					657:667	wound healing application	643:667	wound healing application	643:667	To overcome these issues, a novel type of porous three-dimensional (3D) film was fabricated using chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC) loaded with 45S5 bioglass (CCMPBG) by the freeze-drying method for wound healing application.
34689631	3	3	theme	freeze-drying	618:630	arg1	method					632:637	the freeze-drying method	614:637	the freeze-drying method for wound healing application	614:667	To overcome these issues, a novel type of porous three-dimensional (3D) film was fabricated using chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC) loaded with 45S5 bioglass (CCMPBG) by the freeze-drying method for wound healing application.
34689631	5	4	theme	well-interconnected	861:879	arg1	micropores					881:890	well-interconnected micropores	861:890	well-interconnected micropores	861:890	The CCMPBG films exhibited rough surface morphology and well-interconnected micropores with an average size range of 101-74 μm.
34689631	6	5	theme	pullulan	980:987	arg1	film					996:999	the control chitosan/carboxymethyl pullulan (CCMP) film	945:999	the control chitosan/carboxymethyl pullulan (CCMP) film	945:999	Compared to the control chitosan/carboxymethyl pullulan (CCMP) film, the CCMPBG films showed an enhanced mechanical strength and controlled rate of swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG).
34689631	6	6	theme	controlled	1062:1071	arg1	rate					1073:1076	controlled rate	1062:1076	controlled rate of swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG)	1062:1182	Compared to the control chitosan/carboxymethyl pullulan (CCMP) film, the CCMPBG films showed an enhanced mechanical strength and controlled rate of swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG).
34689631	8	7	theme	wound	1471:1475	arg1	therapy					1477:1483	wound therapy	1471:1483	wound therapy	1471:1483	The results demonstrated that CCMPBG films can be an effective dressing material for wound therapy.
34689631	5	8	theme	101-74 μm	922:930	arg1	range					913:917	an average size range	897:917	an average size range of 101-74 μm	897:930	The CCMPBG films exhibited rough surface morphology and well-interconnected micropores with an average size range of 101-74 μm.
34689631	6	9	theme	chitosan/carboxymethyl	957:978	arg1	film					996:999	the control chitosan/carboxymethyl pullulan (CCMP) film	945:999	the control chitosan/carboxymethyl pullulan (CCMP) film	945:999	Compared to the control chitosan/carboxymethyl pullulan (CCMP) film, the CCMPBG films showed an enhanced mechanical strength and controlled rate of swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG).
34689631	3	10	theme	polyelectrolyte	546:560	arg1	PEC					571:573	chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC)	514:574	chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC) loaded with 45S5 bioglass (CCMPBG) by the freeze-drying method for wound healing application	514:667	To overcome these issues, a novel type of porous three-dimensional (3D) film was fabricated using chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC) loaded with 45S5 bioglass (CCMPBG) by the freeze-drying method for wound healing application.
34689631	7	11	dep	biocompatibility	1234:1249	arg1	ability					1293:1299	ability	1293:1299	ability	1293:1299	Furthermore, CCMPBG films presented the improved biocompatibility, antimicrobial activity and wound closure ability because of the synergistic effects of chitosan, carboxymethyl pullulan (CMP) and BG.
34689631	6	12	theme	matrix	1154:1159	arg1	interaction					1131:1141	the interaction	1127:1141	the interaction of polymer matrix and 45S5 bioglass (BG)	1127:1182	Compared to the control chitosan/carboxymethyl pullulan (CCMP) film, the CCMPBG films showed an enhanced mechanical strength and controlled rate of swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG).
34689631	5	13	theme	CCMPBG	809:814	arg1	films					816:820	The CCMPBG films	805:820	The CCMPBG films	805:820	The CCMPBG films exhibited rough surface morphology and well-interconnected micropores with an average size range of 101-74 μm.
34689631	6	14	theme	behaviours	1109:1118	arg1	rate					1073:1076	controlled rate	1062:1076	controlled rate of swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG)	1062:1182	Compared to the control chitosan/carboxymethyl pullulan (CCMP) film, the CCMPBG films showed an enhanced mechanical strength and controlled rate of swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG).
34689631	6	14	theme	behaviours	1109:1118	arg1	strength					1049:1056	an enhanced mechanical strength	1026:1056	an enhanced mechanical strength	1026:1056	Compared to the control chitosan/carboxymethyl pullulan (CCMP) film, the CCMPBG films showed an enhanced mechanical strength and controlled rate of swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG).
34689631	2	15	theme	many	266:269	arg1	rigidity					289:296	rigidity	289:296	rigidity	289:296	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	2	15	theme	many	266:269	arg1	activity					406:413	less antimicrobial activity	387:413	less antimicrobial activity	387:413	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	2	15	theme	many	266:269	arg1	non-porosity					299:310	non-porosity	299:310	non-porosity	299:310	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	2	15	theme	many	266:269	arg1	affinity					341:348	an affinity	338:348	an affinity to stick onto the injury surface	338:381	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	2	15	theme	many	266:269	arg1	drawbacks					271:279	many drawbacks	266:279	many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity	266:413	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	2	15	theme	many	266:269	arg1	strength					328:335	low mechanical strength	313:335	low mechanical strength	313:335	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	7	16	theme	synergistic	1316:1326	arg1	effects					1328:1334	the synergistic effects	1312:1334	the synergistic effects of chitosan, carboxymethyl pullulan (CMP) and BG	1312:1383	Furthermore, CCMPBG films presented the improved biocompatibility, antimicrobial activity and wound closure ability because of the synergistic effects of chitosan, carboxymethyl pullulan (CMP) and BG.
34689631	2	17	theme	injury	368:373	arg1	surface					375:381	the injury surface	364:381	the injury surface	364:381	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	6	18	theme	control	949:955	arg1	film					996:999	the control chitosan/carboxymethyl pullulan (CCMP) film	945:999	the control chitosan/carboxymethyl pullulan (CCMP) film	945:999	Compared to the control chitosan/carboxymethyl pullulan (CCMP) film, the CCMPBG films showed an enhanced mechanical strength and controlled rate of swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG).
34689631	4	19	theme	surface	783:789	arg1	morphologies					791:802	surface morphologies	783:802	surface morphologies	783:802	The developed films were analysed by FTIR, XRD, EDS and SEM to confirm their chemical nature, microstructure and surface morphologies.
34689631	5	20	theme	size	908:911	arg1	range					913:917	an average size range	897:917	an average size range of 101-74 μm	897:930	The CCMPBG films exhibited rough surface morphology and well-interconnected micropores with an average size range of 101-74 μm.
34689631	6	21	theme	mechanical	1038:1047	arg1	strength					1049:1056	an enhanced mechanical strength	1026:1056	an enhanced mechanical strength	1026:1056	Compared to the control chitosan/carboxymethyl pullulan (CCMP) film, the CCMPBG films showed an enhanced mechanical strength and controlled rate of swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG).
34689631	1	22	theme	Wound	112:116	arg1	dressings					118:126	Wound dressings	112:126	Wound dressings	112:126	Wound dressings play a vital role in the wound healing process.
34689631	6	23	theme	enhanced	1029:1036	arg1	strength					1049:1056	an enhanced mechanical strength	1026:1056	an enhanced mechanical strength	1026:1056	Compared to the control chitosan/carboxymethyl pullulan (CCMP) film, the CCMPBG films showed an enhanced mechanical strength and controlled rate of swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG).
34689631	7	24	theme	antimicrobial	1252:1264	arg1	activity					1266:1273	antimicrobial activity	1252:1273	antimicrobial activity	1252:1273	Furthermore, CCMPBG films presented the improved biocompatibility, antimicrobial activity and wound closure ability because of the synergistic effects of chitosan, carboxymethyl pullulan (CMP) and BG.
34689631	2	25	contain	have	261:264	arg2	drawbacks					271:279	many drawbacks	266:279	many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity	266:413	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	2	25	contain	have	261:264	arg2	rigidity					289:296	rigidity	289:296	rigidity	289:296	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	2	25	contain	have	261:264	arg2	strength					328:335	low mechanical strength	313:335	low mechanical strength	313:335	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	2	25	contain	have	261:264	arg2	non-porosity					299:310	non-porosity	299:310	non-porosity	299:310	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	2	25	contain	have	261:264	arg1	most					242:245	most	242:245	most	242:245	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	2	25	contain	have	261:264	arg2	activity					406:413	less antimicrobial activity	387:413	less antimicrobial activity	387:413	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	2	25	contain	have	261:264	arg2	affinity					341:348	an affinity	338:348	an affinity to stick onto the injury surface	338:381	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	2	25	contain	have	261:264	arg1	them					250:253	them	250:253	them	250:253	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	8	26	theme	effective	1439:1447	arg1	films					1423:1427	CCMPBG films	1416:1427	CCMPBG films	1416:1427	The results demonstrated that CCMPBG films can be an effective dressing material for wound therapy.
34689631	8	26	theme	effective	1439:1447	arg1	material					1458:1465	an effective dressing material	1436:1465	an effective dressing material for wound therapy	1436:1483	The results demonstrated that CCMPBG films can be an effective dressing material for wound therapy.
34689631	3	27	theme	complex	562:568	arg1	PEC					571:573	chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC)	514:574	chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC) loaded with 45S5 bioglass (CCMPBG) by the freeze-drying method for wound healing application	514:667	To overcome these issues, a novel type of porous three-dimensional (3D) film was fabricated using chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC) loaded with 45S5 bioglass (CCMPBG) by the freeze-drying method for wound healing application.
34689631	5	28	theme	rough	832:836	arg1	morphology					846:855	rough surface morphology	832:855	rough surface morphology	832:855	The CCMPBG films exhibited rough surface morphology and well-interconnected micropores with an average size range of 101-74 μm.
34689631	8	29	theme	dressing	1449:1456	arg1	films					1423:1427	CCMPBG films	1416:1427	CCMPBG films	1416:1427	The results demonstrated that CCMPBG films can be an effective dressing material for wound therapy.
34689631	8	29	theme	dressing	1449:1456	arg1	material					1458:1465	an effective dressing material	1436:1465	an effective dressing material for wound therapy	1436:1483	The results demonstrated that CCMPBG films can be an effective dressing material for wound therapy.
34689631	6	30	theme	bioglass	1170:1177	arg1	interaction					1131:1141	the interaction	1127:1141	the interaction of polymer matrix and 45S5 bioglass (BG)	1127:1182	Compared to the control chitosan/carboxymethyl pullulan (CCMP) film, the CCMPBG films showed an enhanced mechanical strength and controlled rate of swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG).
34689631	3	31	theme	chitosan/carboxymethyl	514:535	arg1	PEC					571:573	chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC)	514:574	chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC) loaded with 45S5 bioglass (CCMPBG) by the freeze-drying method for wound healing application	514:667	To overcome these issues, a novel type of porous three-dimensional (3D) film was fabricated using chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC) loaded with 45S5 bioglass (CCMPBG) by the freeze-drying method for wound healing application.
34689631	2	32	theme	antimicrobial	392:404	arg1	activity					406:413	less antimicrobial activity	387:413	less antimicrobial activity	387:413	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	6	33	theme	swelling	1081:1088	arg1	behaviours					1109:1118	swelling and biodegradation behaviours	1081:1118	swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG)	1081:1182	Compared to the control chitosan/carboxymethyl pullulan (CCMP) film, the CCMPBG films showed an enhanced mechanical strength and controlled rate of swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG).
34689631	3	34	theme	film	488:491	arg1	type					450:453	a novel type	442:453	a novel type of porous three-dimensional (3D) film	442:491	To overcome these issues, a novel type of porous three-dimensional (3D) film was fabricated using chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC) loaded with 45S5 bioglass (CCMPBG) by the freeze-drying method for wound healing application.
34689631	8	35	theme	CCMPBG	1416:1421	arg1	films					1423:1427	CCMPBG films	1416:1427	CCMPBG films	1416:1427	The results demonstrated that CCMPBG films can be an effective dressing material for wound therapy.
34689631	8	35	theme	CCMPBG	1416:1421	arg1	material					1458:1465	an effective dressing material	1436:1465	an effective dressing material for wound therapy	1436:1483	The results demonstrated that CCMPBG films can be an effective dressing material for wound therapy.
34689631	3	36	theme	pullulan	537:544	arg1	PEC					571:573	chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC)	514:574	chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC) loaded with 45S5 bioglass (CCMPBG) by the freeze-drying method for wound healing application	514:667	To overcome these issues, a novel type of porous three-dimensional (3D) film was fabricated using chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC) loaded with 45S5 bioglass (CCMPBG) by the freeze-drying method for wound healing application.
34689631	5	37	theme	surface	838:844	arg1	morphology					846:855	rough surface morphology	832:855	rough surface morphology	832:855	The CCMPBG films exhibited rough surface morphology and well-interconnected micropores with an average size range of 101-74 μm.
34689631	3	38	theme	porous	458:463	arg1	film					488:491	porous three-dimensional (3D) film	458:491	porous three-dimensional (3D) film	458:491	To overcome these issues, a novel type of porous three-dimensional (3D) film was fabricated using chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC) loaded with 45S5 bioglass (CCMPBG) by the freeze-drying method for wound healing application.
34689631	6	39	theme	biodegradation	1094:1107	arg1	behaviours					1109:1118	swelling and biodegradation behaviours	1081:1118	swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG)	1081:1182	Compared to the control chitosan/carboxymethyl pullulan (CCMP) film, the CCMPBG films showed an enhanced mechanical strength and controlled rate of swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG).
34689631	7	40	theme	pullulan	1363:1370	arg1	effects					1328:1334	the synergistic effects	1312:1334	the synergistic effects of chitosan, carboxymethyl pullulan (CMP) and BG	1312:1383	Furthermore, CCMPBG films presented the improved biocompatibility, antimicrobial activity and wound closure ability because of the synergistic effects of chitosan, carboxymethyl pullulan (CMP) and BG.
34689631	6	41	theme	CCMPBG	1006:1011	arg1	films					1013:1017	the CCMPBG films	1002:1017	the CCMPBG films	1002:1017	Compared to the control chitosan/carboxymethyl pullulan (CCMP) film, the CCMPBG films showed an enhanced mechanical strength and controlled rate of swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG).
34689631	0	42	theme	chitosan/carboxymethyl	36:57	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of chitosan/carboxymethyl pullulan/bioglass composite films for wound healing.
34689631	0	42	theme	chitosan/carboxymethyl	36:57	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of chitosan/carboxymethyl pullulan/bioglass composite films for wound healing.
34689631	3	43	dep	three-dimensional	465:481	arg1	3D					484:485	3D	484:485	3D	484:485	To overcome these issues, a novel type of porous three-dimensional (3D) film was fabricated using chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC) loaded with 45S5 bioglass (CCMPBG) by the freeze-drying method for wound healing application.
34689631	6	44	theme	CCMP	990:993	arg1	film					996:999	the control chitosan/carboxymethyl pullulan (CCMP) film	945:999	the control chitosan/carboxymethyl pullulan (CCMP) film	945:999	Compared to the control chitosan/carboxymethyl pullulan (CCMP) film, the CCMPBG films showed an enhanced mechanical strength and controlled rate of swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG).
34689631	1	45	theme	vital	135:139	arg1	role					141:144	a vital role	133:144	a vital role	133:144	Wound dressings play a vital role in the wound healing process.
34689631	0	46	theme	composite	77:85	arg1	films					87:91	composite films	77:91	composite films for wound healing	77:109	Preparation and characterization of chitosan/carboxymethyl pullulan/bioglass composite films for wound healing.
34689631	7	47	theme	carboxymethyl	1349:1361	arg1	CMP					1373:1375	CMP	1373:1375	CMP	1373:1375	Furthermore, CCMPBG films presented the improved biocompatibility, antimicrobial activity and wound closure ability because of the synergistic effects of chitosan, carboxymethyl pullulan (CMP) and BG.
34689631	7	47	theme	carboxymethyl	1349:1361	arg1	pullulan					1363:1370	carboxymethyl pullulan	1349:1370	carboxymethyl pullulan (CMP)	1349:1376	Furthermore, CCMPBG films presented the improved biocompatibility, antimicrobial activity and wound closure ability because of the synergistic effects of chitosan, carboxymethyl pullulan (CMP) and BG.
34689631	2	48	theme	mechanical	317:326	arg1	strength					328:335	low mechanical strength	313:335	low mechanical strength	313:335	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	6	49	theme	45S5	1165:1168	arg1	BG					1180:1181	BG	1180:1181	BG	1180:1181	Compared to the control chitosan/carboxymethyl pullulan (CCMP) film, the CCMPBG films showed an enhanced mechanical strength and controlled rate of swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG).
34689631	6	49	theme	45S5	1165:1168	arg1	bioglass					1170:1177	45S5 bioglass	1165:1177	45S5 bioglass (BG)	1165:1182	Compared to the control chitosan/carboxymethyl pullulan (CCMP) film, the CCMPBG films showed an enhanced mechanical strength and controlled rate of swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG).
34689631	3	50	theme	wound	643:647	arg1	healing					649:655	wound healing	643:655	wound healing application	643:667	To overcome these issues, a novel type of porous three-dimensional (3D) film was fabricated using chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC) loaded with 45S5 bioglass (CCMPBG) by the freeze-drying method for wound healing application.
34689631	2	51	theme	low	313:315	arg1	strength					328:335	low mechanical strength	313:335	low mechanical strength	313:335	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	2	52	theme	dressings	204:212	arg1	variety					187:193	a variety	185:193	a variety of wound dressings	185:212	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	2	52	theme	dressings	204:212	arg1	dressings					204:212	wound dressings	198:212	wound dressings	198:212	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	7	53	theme	chitosan	1339:1346	arg1	effects					1328:1334	the synergistic effects	1312:1334	the synergistic effects of chitosan, carboxymethyl pullulan (CMP) and BG	1312:1383	Furthermore, CCMPBG films presented the improved biocompatibility, antimicrobial activity and wound closure ability because of the synergistic effects of chitosan, carboxymethyl pullulan (CMP) and BG.
34689631	5	54	theme	average	900:906	arg1	range					913:917	an average size range	897:917	an average size range of 101-74 μm	897:930	The CCMPBG films exhibited rough surface morphology and well-interconnected micropores with an average size range of 101-74 μm.
34689631	3	55	theme	novel	444:448	arg1	type					450:453	a novel type	442:453	a novel type of porous three-dimensional (3D) film	442:491	To overcome these issues, a novel type of porous three-dimensional (3D) film was fabricated using chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC) loaded with 45S5 bioglass (CCMPBG) by the freeze-drying method for wound healing application.
34689631	0	56	theme	wound	97:101	arg1	healing					103:109	wound healing	97:109	wound healing	97:109	Preparation and characterization of chitosan/carboxymethyl pullulan/bioglass composite films for wound healing.
34689631	2	57	theme	wound	198:202	arg1	dressings					204:212	wound dressings	198:212	wound dressings	198:212	Although a variety of wound dressings have been developed so far, most of them still have many drawbacks such as rigidity, non-porosity, low mechanical strength, an affinity to stick onto the injury surface and less antimicrobial activity.
34689631	4	58	theme	chemical	747:754	arg1	nature					756:761	their chemical nature	741:761	their chemical nature	741:761	The developed films were analysed by FTIR, XRD, EDS and SEM to confirm their chemical nature, microstructure and surface morphologies.
34689631	7	59	theme	wound	1279:1283	arg1	closure					1285:1291	wound closure	1279:1291	wound closure	1279:1291	Furthermore, CCMPBG films presented the improved biocompatibility, antimicrobial activity and wound closure ability because of the synergistic effects of chitosan, carboxymethyl pullulan (CMP) and BG.
34689631	1	60	theme	wound	153:157	arg1	process					167:173	the wound healing process	149:173	the wound healing process	149:173	Wound dressings play a vital role in the wound healing process.
34689631	6	61	theme	polymer	1146:1152	arg1	matrix					1154:1159	polymer matrix	1146:1159	polymer matrix	1146:1159	Compared to the control chitosan/carboxymethyl pullulan (CCMP) film, the CCMPBG films showed an enhanced mechanical strength and controlled rate of swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG).
34689631	6	62	theme	due	1120:1122	arg1	behaviours					1109:1118	swelling and biodegradation behaviours	1081:1118	swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG)	1081:1182	Compared to the control chitosan/carboxymethyl pullulan (CCMP) film, the CCMPBG films showed an enhanced mechanical strength and controlled rate of swelling and biodegradation behaviours due to the interaction of polymer matrix and 45S5 bioglass (BG).
34689631	3	63	theme	45S5	588:591	arg1	CCMPBG					603:608	CCMPBG	603:608	CCMPBG	603:608	To overcome these issues, a novel type of porous three-dimensional (3D) film was fabricated using chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC) loaded with 45S5 bioglass (CCMPBG) by the freeze-drying method for wound healing application.
34689631	3	63	theme	45S5	588:591	arg1	bioglass					593:600	45S5 bioglass	588:600	45S5 bioglass (CCMPBG)	588:609	To overcome these issues, a novel type of porous three-dimensional (3D) film was fabricated using chitosan/carboxymethyl pullulan polyelectrolyte complex (PEC) loaded with 45S5 bioglass (CCMPBG) by the freeze-drying method for wound healing application.
34689631	7	64	theme	improved	1225:1232	arg1	biocompatibility					1234:1249	the improved biocompatibility	1221:1249	the improved biocompatibility	1221:1249	Furthermore, CCMPBG films presented the improved biocompatibility, antimicrobial activity and wound closure ability because of the synergistic effects of chitosan, carboxymethyl pullulan (CMP) and BG.
34689631	1	65	theme	healing	159:165	arg1	process					167:173	the wound healing process	149:173	the wound healing process	149:173	Wound dressings play a vital role in the wound healing process.
34689631	7	66	theme	CCMPBG	1198:1203	arg1	films					1205:1209	CCMPBG films	1198:1209	CCMPBG films	1198:1209	Furthermore, CCMPBG films presented the improved biocompatibility, antimicrobial activity and wound closure ability because of the synergistic effects of chitosan, carboxymethyl pullulan (CMP) and BG.
34689631	4	67	theme	developed	674:682	arg1	films					684:688	The developed films	670:688	The developed films	670:688	The developed films were analysed by FTIR, XRD, EDS and SEM to confirm their chemical nature, microstructure and surface morphologies.
32092423	4	0	theme	equilibrium	509:519	arg1	studies					521:527	Kinetic and adsorption equilibrium studies	486:527	Kinetic and adsorption equilibrium studies	486:527	Kinetic and adsorption equilibrium studies showed that the pseudo-second-order and Sips isotherm models adjusted well to the experimental data, respectively.
32092423	5	1	theme	Cu	711:712	arg1	removal					718:724	Cu(II) removal	711:724	Cu(II) removal of 29.20 mg g-1	711:740	Langmuir isotherm indicated a maximum adsorption capacity (qm) for Cu(II) removal of 29.20 mg g-1.
32092423	6	2	theme	Differential	743:754	arg1	calorimetry					765:775	Differential scanning calorimetry	743:775	Differential scanning calorimetry	743:775	Differential scanning calorimetry, contact angle measurements, and X-ray photoelectron spectroscopy confirm the adsorption.
32092423	2	3	theme	degree	324:329	arg1	pectin					293:298	pectin	293:298	pectin of high O-methoxylation degree (56%)	293:335	We overcome this issue by blending chitosan to pectin of high O-methoxylation degree (56%), followed by solvent evaporation.
32092423	1	4	theme	polyelectrolyte	220:234	arg1	mixtures					236:243	polyelectrolyte mixtures	220:243	polyelectrolyte mixtures	220:243	To obtain pectin-based films is challenging due to the aqueous instability of polyelectrolyte mixtures.
32092423	5	5	theme	maximum	674:680	arg1	capacity					693:700	a maximum adsorption capacity	672:700	a maximum adsorption capacity (qm) for Cu(II) removal of 29.20 mg g-1	672:740	Langmuir isotherm indicated a maximum adsorption capacity (qm) for Cu(II) removal of 29.20 mg g-1.
32092423	5	5	theme	maximum	674:680	arg1	qm					703:704	qm	703:704	qm	703:704	Langmuir isotherm indicated a maximum adsorption capacity (qm) for Cu(II) removal of 29.20 mg g-1.
32092423	1	6	theme	mixtures	236:243	arg1	instability					205:215	the aqueous instability	193:215	the aqueous instability of polyelectrolyte mixtures	193:243	To obtain pectin-based films is challenging due to the aqueous instability of polyelectrolyte mixtures.
32092423	0	7	theme	pectin-based	55:66	arg1	film					68:71	a pectin-based film	53:71	a pectin-based film	53:71	Removal of Cu(II) from aqueous solutions imparted by a pectin-based film: Cytocompatibility, antimicrobial, kinetic, and equilibrium studies.
32092423	8	8	theme	cytocompatible	985:998	arg1	pair					1012:1015	the cytocompatible film/Cu(II) pair	981:1015	the cytocompatible film/Cu(II) pair	981:1015	After adsorption, the cytocompatible film/Cu(II) pair prevents the proliferation of Escherichia coli.
32092423	3	9	theme	Cu	473:474	arg1	ions					480:483	Cu(II) ions	473:483	Cu(II) ions	473:483	A durable film containing 74 wt% pectin content was produced and used as an adsorbent material toward Cu(II) ions.
32092423	4	10	theme	experimental	611:622	arg1	data					624:627	the experimental data	607:627	the experimental data	607:627	Kinetic and adsorption equilibrium studies showed that the pseudo-second-order and Sips isotherm models adjusted well to the experimental data, respectively.
32092423	6	11	theme	photoelectron	816:828	arg1	spectroscopy					830:841	X-ray photoelectron spectroscopy	810:841	X-ray photoelectron spectroscopy	810:841	Differential scanning calorimetry, contact angle measurements, and X-ray photoelectron spectroscopy confirm the adsorption.
32092423	6	12	theme	angle	786:790	arg1	measurements					792:803	contact angle measurements	778:803	contact angle measurements	778:803	Differential scanning calorimetry, contact angle measurements, and X-ray photoelectron spectroscopy confirm the adsorption.
32092423	3	13	theme	74 wt	397:401	arg1	content					411:417	74 wt% pectin content	397:417	74 wt% pectin content	397:417	A durable film containing 74 wt% pectin content was produced and used as an adsorbent material toward Cu(II) ions.
32092423	6	14	theme	X-ray	810:814	arg1	spectroscopy					830:841	X-ray photoelectron spectroscopy	810:841	X-ray photoelectron spectroscopy	810:841	Differential scanning calorimetry, contact angle measurements, and X-ray photoelectron spectroscopy confirm the adsorption.
32092423	7	15	theme	essential	894:902	arg1	role					904:907	an essential role	891:907	an essential role	891:907	The chemisorption plays an essential role in the process; thereby, the film reusability is low.
32092423	3	16	theme	durable	373:379	arg1	material					457:464	an adsorbent material	444:464	an adsorbent material toward Cu(II) ions	444:483	A durable film containing 74 wt% pectin content was produced and used as an adsorbent material toward Cu(II) ions.
32092423	3	16	theme	durable	373:379	arg1	film					381:384	A durable film	371:384	A durable film containing 74 wt% pectin content	371:417	A durable film containing 74 wt% pectin content was produced and used as an adsorbent material toward Cu(II) ions.
32092423	5	17	theme	29.20 mg g-1	729:740	arg1	removal					718:724	Cu(II) removal	711:724	Cu(II) removal of 29.20 mg g-1	711:740	Langmuir isotherm indicated a maximum adsorption capacity (qm) for Cu(II) removal of 29.20 mg g-1.
32092423	6	18	theme	scanning	756:763	arg1	calorimetry					765:775	Differential scanning calorimetry	743:775	Differential scanning calorimetry	743:775	Differential scanning calorimetry, contact angle measurements, and X-ray photoelectron spectroscopy confirm the adsorption.
32092423	1	19	theme	pectin-based	152:163	arg1	films					165:169	pectin-based films	152:169	pectin-based films	152:169	To obtain pectin-based films is challenging due to the aqueous instability of polyelectrolyte mixtures.
32092423	5	20	theme	Langmuir	644:651	arg1	isotherm					653:660	Langmuir isotherm	644:660	Langmuir isotherm	644:660	Langmuir isotherm indicated a maximum adsorption capacity (qm) for Cu(II) removal of 29.20 mg g-1.
32092423	0	21	dep	imparted	41:48	arg1	kinetic					108:114	kinetic	108:114	kinetic	108:114	Removal of Cu(II) from aqueous solutions imparted by a pectin-based film: Cytocompatibility, antimicrobial, kinetic, and equilibrium studies.
32092423	0	21	dep	imparted	41:48	arg1	studies					133:139	equilibrium studies	121:139	equilibrium studies	121:139	Removal of Cu(II) from aqueous solutions imparted by a pectin-based film: Cytocompatibility, antimicrobial, kinetic, and equilibrium studies.
32092423	0	21	dep	imparted	41:48	arg1	antimicrobial					93:105	antimicrobial	93:105	antimicrobial	93:105	Removal of Cu(II) from aqueous solutions imparted by a pectin-based film: Cytocompatibility, antimicrobial, kinetic, and equilibrium studies.
32092423	0	21	dep	imparted	41:48	arg1	Cytocompatibility					74:90	Cytocompatibility	74:90	Cytocompatibility	74:90	Removal of Cu(II) from aqueous solutions imparted by a pectin-based film: Cytocompatibility, antimicrobial, kinetic, and equilibrium studies.
32092423	2	22	theme	solvent	350:356	arg1	evaporation					358:368	solvent evaporation	350:368	solvent evaporation	350:368	We overcome this issue by blending chitosan to pectin of high O-methoxylation degree (56%), followed by solvent evaporation.
32092423	0	23	theme	Cu	11:12	arg1	Removal					0:6	Removal	0:6	Removal of Cu(II) from aqueous solutions	0:39	Removal of Cu(II) from aqueous solutions imparted by a pectin-based film: Cytocompatibility, antimicrobial, kinetic, and equilibrium studies.
32092423	3	24	theme	adsorbent	447:455	arg1	material					457:464	an adsorbent material	444:464	an adsorbent material toward Cu(II) ions	444:483	A durable film containing 74 wt% pectin content was produced and used as an adsorbent material toward Cu(II) ions.
32092423	3	24	theme	adsorbent	447:455	arg1	film					381:384	A durable film	371:384	A durable film containing 74 wt% pectin content	371:417	A durable film containing 74 wt% pectin content was produced and used as an adsorbent material toward Cu(II) ions.
32092423	4	25	theme	Sips	569:572	arg1	models					583:588	the pseudo-second-order and Sips isotherm models	541:588	models	583:588	Kinetic and adsorption equilibrium studies showed that the pseudo-second-order and Sips isotherm models adjusted well to the experimental data, respectively.
32092423	4	26	theme	Kinetic	486:492	arg1	studies					521:527	Kinetic and adsorption equilibrium studies	486:527	Kinetic and adsorption equilibrium studies	486:527	Kinetic and adsorption equilibrium studies showed that the pseudo-second-order and Sips isotherm models adjusted well to the experimental data, respectively.
32092423	3	27	theme	%	402:402	arg1	content					411:417	74 wt% pectin content	397:417	74 wt% pectin content	397:417	A durable film containing 74 wt% pectin content was produced and used as an adsorbent material toward Cu(II) ions.
32092423	4	28	theme	adsorption	498:507	arg1	equilibrium					509:519	adsorption equilibrium	498:519	adsorption equilibrium	498:519	Kinetic and adsorption equilibrium studies showed that the pseudo-second-order and Sips isotherm models adjusted well to the experimental data, respectively.
32092423	3	29	theme	pectin	404:409	arg1	content					411:417	74 wt% pectin content	397:417	74 wt% pectin content	397:417	A durable film containing 74 wt% pectin content was produced and used as an adsorbent material toward Cu(II) ions.
32092423	0	30	theme	aqueous	23:29	arg1	solutions					31:39	aqueous solutions	23:39	aqueous solutions	23:39	Removal of Cu(II) from aqueous solutions imparted by a pectin-based film: Cytocompatibility, antimicrobial, kinetic, and equilibrium studies.
32092423	0	31	from	solutions	31:39	arg1	Removal					0:6	Removal	0:6	Removal of Cu(II) from aqueous solutions	0:39	Removal of Cu(II) from aqueous solutions imparted by a pectin-based film: Cytocompatibility, antimicrobial, kinetic, and equilibrium studies.
32092423	2	32	theme	O-methoxylation	308:322	arg1	degree					324:329	high O-methoxylation degree	303:329	high O-methoxylation degree (56%)	303:335	We overcome this issue by blending chitosan to pectin of high O-methoxylation degree (56%), followed by solvent evaporation.
32092423	2	32	theme	O-methoxylation	308:322	arg1	%					334:334	56%	332:334	56%	332:334	We overcome this issue by blending chitosan to pectin of high O-methoxylation degree (56%), followed by solvent evaporation.
32092423	6	33	theme	contact	778:784	arg1	measurements					792:803	contact angle measurements	778:803	contact angle measurements	778:803	Differential scanning calorimetry, contact angle measurements, and X-ray photoelectron spectroscopy confirm the adsorption.
32092423	3	34	used	used	436:439	arg2	material					457:464	an adsorbent material	444:464	an adsorbent material toward Cu(II) ions	444:483	A durable film containing 74 wt% pectin content was produced and used as an adsorbent material toward Cu(II) ions.
32092423	3	34	used	used	436:439	arg2	film					381:384	A durable film	371:384	A durable film containing 74 wt% pectin content	371:417	A durable film containing 74 wt% pectin content was produced and used as an adsorbent material toward Cu(II) ions.
32092423	7	35	theme	film	938:941	arg1	reusability					943:953	the film reusability	934:953	the film reusability	934:953	The chemisorption plays an essential role in the process; thereby, the film reusability is low.
32092423	7	35	theme	film	938:941	arg1	low					958:960	low	958:960	low	958:960	The chemisorption plays an essential role in the process; thereby, the film reusability is low.
32092423	2	36	theme	high	303:306	arg1	degree					324:329	high O-methoxylation degree	303:329	high O-methoxylation degree (56%)	303:335	We overcome this issue by blending chitosan to pectin of high O-methoxylation degree (56%), followed by solvent evaporation.
32092423	2	36	theme	high	303:306	arg1	%					334:334	56%	332:334	56%	332:334	We overcome this issue by blending chitosan to pectin of high O-methoxylation degree (56%), followed by solvent evaporation.
32092423	0	37	theme	equilibrium	121:131	arg1	studies					133:139	equilibrium studies	121:139	equilibrium studies	121:139	Removal of Cu(II) from aqueous solutions imparted by a pectin-based film: Cytocompatibility, antimicrobial, kinetic, and equilibrium studies.
32092423	8	38	theme	film/Cu	1000:1006	arg1	pair					1012:1015	the cytocompatible film/Cu(II) pair	981:1015	the cytocompatible film/Cu(II) pair	981:1015	After adsorption, the cytocompatible film/Cu(II) pair prevents the proliferation of Escherichia coli.
32092423	3	39	contain	containing	386:395	arg2	content					411:417	74 wt% pectin content	397:417	74 wt% pectin content	397:417	A durable film containing 74 wt% pectin content was produced and used as an adsorbent material toward Cu(II) ions.
32092423	3	39	contain	containing	386:395	arg1	material					457:464	an adsorbent material	444:464	an adsorbent material toward Cu(II) ions	444:483	A durable film containing 74 wt% pectin content was produced and used as an adsorbent material toward Cu(II) ions.
32092423	3	39	contain	containing	386:395	arg1	film					381:384	A durable film	371:384	A durable film containing 74 wt% pectin content	371:417	A durable film containing 74 wt% pectin content was produced and used as an adsorbent material toward Cu(II) ions.
32092423	4	40	theme	isotherm	574:581	arg1	models					583:588	the pseudo-second-order and Sips isotherm models	541:588	models	583:588	Kinetic and adsorption equilibrium studies showed that the pseudo-second-order and Sips isotherm models adjusted well to the experimental data, respectively.
32092423	1	41	theme	aqueous	197:203	arg1	instability					205:215	the aqueous instability	193:215	the aqueous instability of polyelectrolyte mixtures	193:243	To obtain pectin-based films is challenging due to the aqueous instability of polyelectrolyte mixtures.
32092423	8	42	theme	coli	1059:1062	arg1	proliferation					1030:1042	the proliferation	1026:1042	the proliferation of Escherichia coli	1026:1062	After adsorption, the cytocompatible film/Cu(II) pair prevents the proliferation of Escherichia coli.
32092423	5	43	theme	adsorption	682:691	arg1	capacity					693:700	a maximum adsorption capacity	672:700	a maximum adsorption capacity (qm) for Cu(II) removal of 29.20 mg g-1	672:740	Langmuir isotherm indicated a maximum adsorption capacity (qm) for Cu(II) removal of 29.20 mg g-1.
32092423	5	43	theme	adsorption	682:691	arg1	qm					703:704	qm	703:704	qm	703:704	Langmuir isotherm indicated a maximum adsorption capacity (qm) for Cu(II) removal of 29.20 mg g-1.
32768478	2	0	theme	Chitosan	464:471	arg1	amino					441:445	the amino	437:445	the amino of Carboxymethyl Chitosan (CMCS)	437:478	In this study, a simple and non-toxic method was developed to prepare a new type of composite hydrogel, which was formed through the Schiff-base reaction between the aldehyde of Oxidized Hydroxyethyl Cellulose (OHEC) and the amino of Carboxymethyl Chitosan (CMCS).
32768478	2	0	theme	Chitosan	464:471	arg1	aldehyde					382:389	the aldehyde	378:389	the aldehyde of Oxidized Hydroxyethyl Cellulose (OHEC)	378:431	In this study, a simple and non-toxic method was developed to prepare a new type of composite hydrogel, which was formed through the Schiff-base reaction between the aldehyde of Oxidized Hydroxyethyl Cellulose (OHEC) and the amino of Carboxymethyl Chitosan (CMCS).
32768478	2	1	theme	Carboxymethyl	450:462	arg1	CMCS					474:477	CMCS	474:477	CMCS	474:477	In this study, a simple and non-toxic method was developed to prepare a new type of composite hydrogel, which was formed through the Schiff-base reaction between the aldehyde of Oxidized Hydroxyethyl Cellulose (OHEC) and the amino of Carboxymethyl Chitosan (CMCS).
32768478	2	1	theme	Carboxymethyl	450:462	arg1	Chitosan					464:471	Carboxymethyl Chitosan	450:471	Carboxymethyl Chitosan (CMCS)	450:478	In this study, a simple and non-toxic method was developed to prepare a new type of composite hydrogel, which was formed through the Schiff-base reaction between the aldehyde of Oxidized Hydroxyethyl Cellulose (OHEC) and the amino of Carboxymethyl Chitosan (CMCS).
32768478	4	2	theme	composite	654:662	arg1	hydrogel					664:671	this composite hydrogel	649:671	this composite hydrogel	649:671	Statistics achieved from those tests showed that this composite hydrogel comprised of some high-quality properties such as suitable gelation time, good swelling ability, suitable water evaporation rate, good blood compatibility and biocompatibility.
32768478	4	3	theme	swelling	752:759	arg1	ability					761:767	good swelling ability	747:767	good swelling ability	747:767	Statistics achieved from those tests showed that this composite hydrogel comprised of some high-quality properties such as suitable gelation time, good swelling ability, suitable water evaporation rate, good blood compatibility and biocompatibility.
32768478	4	4	theme	gelation	732:739	arg1	time					741:744	suitable gelation time	723:744	suitable gelation time	723:744	Statistics achieved from those tests showed that this composite hydrogel comprised of some high-quality properties such as suitable gelation time, good swelling ability, suitable water evaporation rate, good blood compatibility and biocompatibility.
32768478	3	5	theme	tests	500:504	arg1	series					490:495	a series	488:495	a series of tests toward this new composite hydrogel which contained its structure and performance	488:585	Hence, a series of tests toward this new composite hydrogel which contained its structure and performance was applied.
32768478	3	6	contain	contained	547:555	arg1	hydrogel					532:539	this new composite hydrogel	513:539	this new composite hydrogel which contained its structure and performance	513:585	Hence, a series of tests toward this new composite hydrogel which contained its structure and performance was applied.
32768478	3	6	contain	contained	547:555	arg2	performance					575:585	performance	575:585	performance	575:585	Hence, a series of tests toward this new composite hydrogel which contained its structure and performance was applied.
32768478	3	6	contain	contained	547:555	arg2	structure					561:569	structure	561:569	structure	561:569	Hence, a series of tests toward this new composite hydrogel which contained its structure and performance was applied.
32768478	4	7	theme	blood	808:812	arg1	compatibility					814:826	good blood compatibility	803:826	good blood compatibility	803:826	Statistics achieved from those tests showed that this composite hydrogel comprised of some high-quality properties such as suitable gelation time, good swelling ability, suitable water evaporation rate, good blood compatibility and biocompatibility.
32768478	1	8	theme	Hydrogel	95:102	arg1	dressing					110:117	Hydrogel wound dressing	95:117	Hydrogel wound dressing	95:117	Hydrogel wound dressing is a type of hydrophilic polymer, which has been widely studied and applied in biomedical field.
32768478	1	8	theme	Hydrogel	95:102	arg1	type					124:127	a type	122:127	a type	122:127	Hydrogel wound dressing is a type of hydrophilic polymer, which has been widely studied and applied in biomedical field.
32768478	4	9	theme	good	803:806	arg1	compatibility					814:826	good blood compatibility	803:826	good blood compatibility	803:826	Statistics achieved from those tests showed that this composite hydrogel comprised of some high-quality properties such as suitable gelation time, good swelling ability, suitable water evaporation rate, good blood compatibility and biocompatibility.
32768478	1	10	theme	wound	104:108	arg1	dressing					110:117	Hydrogel wound dressing	95:117	Hydrogel wound dressing	95:117	Hydrogel wound dressing is a type of hydrophilic polymer, which has been widely studied and applied in biomedical field.
32768478	1	10	theme	wound	104:108	arg1	type					124:127	a type	122:127	a type	122:127	Hydrogel wound dressing is a type of hydrophilic polymer, which has been widely studied and applied in biomedical field.
32768478	3	11	theme	new	518:520	arg1	hydrogel					532:539	this new composite hydrogel	513:539	this new composite hydrogel which contained its structure and performance	513:585	Hence, a series of tests toward this new composite hydrogel which contained its structure and performance was applied.
32768478	3	12	theme	composite	522:530	arg1	hydrogel					532:539	this new composite hydrogel	513:539	this new composite hydrogel which contained its structure and performance	513:585	Hence, a series of tests toward this new composite hydrogel which contained its structure and performance was applied.
32768478	2	13	theme	Schiff-base	349:359	arg1	reaction					361:368	the Schiff-base reaction	345:368	the Schiff-base reaction	345:368	In this study, a simple and non-toxic method was developed to prepare a new type of composite hydrogel, which was formed through the Schiff-base reaction between the aldehyde of Oxidized Hydroxyethyl Cellulose (OHEC) and the amino of Carboxymethyl Chitosan (CMCS).
32768478	2	14	theme	new	288:290	arg1	type					292:295	a new type	286:295	a new type of composite hydrogel, which was formed through the Schiff-base reaction between the aldehyde of Oxidized Hydroxyethyl Cellulose (OHEC) and the amino of Carboxymethyl Chitosan (CMCS)	286:478	In this study, a simple and non-toxic method was developed to prepare a new type of composite hydrogel, which was formed through the Schiff-base reaction between the aldehyde of Oxidized Hydroxyethyl Cellulose (OHEC) and the amino of Carboxymethyl Chitosan (CMCS).
32768478	2	15	theme	Hydroxyethyl	403:414	arg1	OHEC					427:430	OHEC	427:430	OHEC	427:430	In this study, a simple and non-toxic method was developed to prepare a new type of composite hydrogel, which was formed through the Schiff-base reaction between the aldehyde of Oxidized Hydroxyethyl Cellulose (OHEC) and the amino of Carboxymethyl Chitosan (CMCS).
32768478	2	15	theme	Hydroxyethyl	403:414	arg1	Cellulose					416:424	Oxidized Hydroxyethyl Cellulose	394:424	Oxidized Hydroxyethyl Cellulose (OHEC)	394:431	In this study, a simple and non-toxic method was developed to prepare a new type of composite hydrogel, which was formed through the Schiff-base reaction between the aldehyde of Oxidized Hydroxyethyl Cellulose (OHEC) and the amino of Carboxymethyl Chitosan (CMCS).
32768478	2	16	theme	Cellulose	416:424	arg1	amino					441:445	the amino	437:445	the amino of Carboxymethyl Chitosan (CMCS)	437:478	In this study, a simple and non-toxic method was developed to prepare a new type of composite hydrogel, which was formed through the Schiff-base reaction between the aldehyde of Oxidized Hydroxyethyl Cellulose (OHEC) and the amino of Carboxymethyl Chitosan (CMCS).
32768478	2	16	theme	Cellulose	416:424	arg1	aldehyde					382:389	the aldehyde	378:389	the aldehyde of Oxidized Hydroxyethyl Cellulose (OHEC)	378:431	In this study, a simple and non-toxic method was developed to prepare a new type of composite hydrogel, which was formed through the Schiff-base reaction between the aldehyde of Oxidized Hydroxyethyl Cellulose (OHEC) and the amino of Carboxymethyl Chitosan (CMCS).
32768478	4	17	theme	good	747:750	arg1	ability					761:767	good swelling ability	747:767	good swelling ability	747:767	Statistics achieved from those tests showed that this composite hydrogel comprised of some high-quality properties such as suitable gelation time, good swelling ability, suitable water evaporation rate, good blood compatibility and biocompatibility.
32768478	5	18	theme	wound	928:932	arg1	dressing					934:941	wound dressing	928:941	wound dressing	928:941	Considering these properties, this hydrogel has a potential to be explored as wound dressing.
32768478	2	19	theme	Oxidized	394:401	arg1	OHEC					427:430	OHEC	427:430	OHEC	427:430	In this study, a simple and non-toxic method was developed to prepare a new type of composite hydrogel, which was formed through the Schiff-base reaction between the aldehyde of Oxidized Hydroxyethyl Cellulose (OHEC) and the amino of Carboxymethyl Chitosan (CMCS).
32768478	2	19	theme	Oxidized	394:401	arg1	Cellulose					416:424	Oxidized Hydroxyethyl Cellulose	394:424	Oxidized Hydroxyethyl Cellulose (OHEC)	394:431	In this study, a simple and non-toxic method was developed to prepare a new type of composite hydrogel, which was formed through the Schiff-base reaction between the aldehyde of Oxidized Hydroxyethyl Cellulose (OHEC) and the amino of Carboxymethyl Chitosan (CMCS).
32768478	4	20	theme	evaporation	785:795	arg1	rate					797:800	suitable water evaporation rate	770:800	suitable water evaporation rate	770:800	Statistics achieved from those tests showed that this composite hydrogel comprised of some high-quality properties such as suitable gelation time, good swelling ability, suitable water evaporation rate, good blood compatibility and biocompatibility.
32768478	0	21	theme	carboxymethyl	30:42	arg1	properties					16:25	properties	16:25	properties	16:25	Preparation and properties of carboxymethyl chitosan/oxidized hydroxyethyl cellulose hydrogel.
32768478	0	21	theme	carboxymethyl	30:42	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and properties of carboxymethyl chitosan/oxidized hydroxyethyl cellulose hydrogel.
32768478	4	22	theme	water	779:783	arg1	rate					797:800	suitable water evaporation rate	770:800	suitable water evaporation rate	770:800	Statistics achieved from those tests showed that this composite hydrogel comprised of some high-quality properties such as suitable gelation time, good swelling ability, suitable water evaporation rate, good blood compatibility and biocompatibility.
32768478	0	23	theme	cellulose	75:83	arg1	hydrogel					85:92	hydroxyethyl cellulose hydrogel	62:92	hydroxyethyl cellulose hydrogel	62:92	Preparation and properties of carboxymethyl chitosan/oxidized hydroxyethyl cellulose hydrogel.
32768478	0	24	theme	hydroxyethyl	62:73	arg1	hydrogel					85:92	hydroxyethyl cellulose hydrogel	62:92	hydroxyethyl cellulose hydrogel	62:92	Preparation and properties of carboxymethyl chitosan/oxidized hydroxyethyl cellulose hydrogel.
32768478	1	25	theme	hydrophilic	132:142	arg1	polymer					144:150	hydrophilic polymer	132:150	hydrophilic polymer	132:150	Hydrogel wound dressing is a type of hydrophilic polymer, which has been widely studied and applied in biomedical field.
32768478	4	26	theme	high-quality	691:702	arg1	compatibility					814:826	good blood compatibility	803:826	good blood compatibility	803:826	Statistics achieved from those tests showed that this composite hydrogel comprised of some high-quality properties such as suitable gelation time, good swelling ability, suitable water evaporation rate, good blood compatibility and biocompatibility.
32768478	4	26	theme	high-quality	691:702	arg1	time					741:744	suitable gelation time	723:744	suitable gelation time	723:744	Statistics achieved from those tests showed that this composite hydrogel comprised of some high-quality properties such as suitable gelation time, good swelling ability, suitable water evaporation rate, good blood compatibility and biocompatibility.
32768478	4	26	theme	high-quality	691:702	arg1	properties					704:713	some high-quality properties	686:713	some high-quality properties such as suitable gelation time, good swelling ability, suitable water evaporation rate, good blood compatibility and biocompatibility	686:847	Statistics achieved from those tests showed that this composite hydrogel comprised of some high-quality properties such as suitable gelation time, good swelling ability, suitable water evaporation rate, good blood compatibility and biocompatibility.
32768478	4	26	theme	high-quality	691:702	arg1	rate					797:800	suitable water evaporation rate	770:800	suitable water evaporation rate	770:800	Statistics achieved from those tests showed that this composite hydrogel comprised of some high-quality properties such as suitable gelation time, good swelling ability, suitable water evaporation rate, good blood compatibility and biocompatibility.
32768478	4	26	theme	high-quality	691:702	arg1	biocompatibility					832:847	biocompatibility	832:847	biocompatibility	832:847	Statistics achieved from those tests showed that this composite hydrogel comprised of some high-quality properties such as suitable gelation time, good swelling ability, suitable water evaporation rate, good blood compatibility and biocompatibility.
32768478	4	26	theme	high-quality	691:702	arg1	ability					761:767	good swelling ability	747:767	good swelling ability	747:767	Statistics achieved from those tests showed that this composite hydrogel comprised of some high-quality properties such as suitable gelation time, good swelling ability, suitable water evaporation rate, good blood compatibility and biocompatibility.
32768478	5	27	contain	has	894:896	arg1	hydrogel					885:892	this hydrogel	880:892	this hydrogel	880:892	Considering these properties, this hydrogel has a potential to be explored as wound dressing.
32768478	5	27	contain	has	894:896	arg2	potential					900:908	a potential	898:908	a potential to be explored as wound dressing	898:941	Considering these properties, this hydrogel has a potential to be explored as wound dressing.
32768478	1	28	theme	polymer	144:150	arg1	dressing					110:117	Hydrogel wound dressing	95:117	Hydrogel wound dressing	95:117	Hydrogel wound dressing is a type of hydrophilic polymer, which has been widely studied and applied in biomedical field.
32768478	1	28	theme	polymer	144:150	arg1	type					124:127	a type	122:127	a type	122:127	Hydrogel wound dressing is a type of hydrophilic polymer, which has been widely studied and applied in biomedical field.
32768478	4	29	theme	suitable	723:730	arg1	time					741:744	suitable gelation time	723:744	suitable gelation time	723:744	Statistics achieved from those tests showed that this composite hydrogel comprised of some high-quality properties such as suitable gelation time, good swelling ability, suitable water evaporation rate, good blood compatibility and biocompatibility.
32768478	2	30	theme	non-toxic	244:252	arg1	method					254:259	a simple and non-toxic method	231:259	a simple and non-toxic method	231:259	In this study, a simple and non-toxic method was developed to prepare a new type of composite hydrogel, which was formed through the Schiff-base reaction between the aldehyde of Oxidized Hydroxyethyl Cellulose (OHEC) and the amino of Carboxymethyl Chitosan (CMCS).
32768478	2	31	theme	composite	300:308	arg1	hydrogel					310:317	composite hydrogel	300:317	composite hydrogel	300:317	In this study, a simple and non-toxic method was developed to prepare a new type of composite hydrogel, which was formed through the Schiff-base reaction between the aldehyde of Oxidized Hydroxyethyl Cellulose (OHEC) and the amino of Carboxymethyl Chitosan (CMCS).
32768478	2	32	theme	hydrogel	310:317	arg1	type					292:295	a new type	286:295	a new type of composite hydrogel, which was formed through the Schiff-base reaction between the aldehyde of Oxidized Hydroxyethyl Cellulose (OHEC) and the amino of Carboxymethyl Chitosan (CMCS)	286:478	In this study, a simple and non-toxic method was developed to prepare a new type of composite hydrogel, which was formed through the Schiff-base reaction between the aldehyde of Oxidized Hydroxyethyl Cellulose (OHEC) and the amino of Carboxymethyl Chitosan (CMCS).
32768478	4	33	theme	suitable	770:777	arg1	rate					797:800	suitable water evaporation rate	770:800	suitable water evaporation rate	770:800	Statistics achieved from those tests showed that this composite hydrogel comprised of some high-quality properties such as suitable gelation time, good swelling ability, suitable water evaporation rate, good blood compatibility and biocompatibility.
32768478	1	34	theme	biomedical	198:207	arg1	field					209:213	biomedical field	198:213	biomedical field	198:213	Hydrogel wound dressing is a type of hydrophilic polymer, which has been widely studied and applied in biomedical field.
32768478	2	35	theme	simple	233:238	arg1	method					254:259	a simple and non-toxic method	231:259	a simple and non-toxic method	231:259	In this study, a simple and non-toxic method was developed to prepare a new type of composite hydrogel, which was formed through the Schiff-base reaction between the aldehyde of Oxidized Hydroxyethyl Cellulose (OHEC) and the amino of Carboxymethyl Chitosan (CMCS).
32896564	5	0	theme	lower	871:875	arg1	temperatures					877:888	lower temperatures	871:888	lower temperatures	871:888	Although composites decomposed at lower temperatures, their thermal stability was sufficient to meet their performance requirements.
32896564	6	1	theme	glass	990:994	arg1	temperature					1007:1017	enhanced glass transition temperature	981:1017	enhanced glass transition temperature (Tg)	981:1022	DSC showed enhanced glass transition temperature (Tg) due to the interfacial interactions between membrane constituents which restrict the mobility of polymer chains.
32896564	6	1	theme	glass	990:994	arg1	Tg					1020:1021	Tg	1020:1021	Tg	1020:1021	DSC showed enhanced glass transition temperature (Tg) due to the interfacial interactions between membrane constituents which restrict the mobility of polymer chains.
32896564	8	2	theme	higher	1323:1328	arg1	permeability					1330:1341	higher permeability	1323:1341	higher permeability	1323:1341	Inclusion of MMCC increased the porosity and hydrophilicity of the membrane which consequently, higher permeability can be achieved.
32896564	6	3	theme	enhanced	981:988	arg1	temperature					1007:1017	enhanced glass transition temperature	981:1017	enhanced glass transition temperature (Tg)	981:1022	DSC showed enhanced glass transition temperature (Tg) due to the interfacial interactions between membrane constituents which restrict the mobility of polymer chains.
32896564	6	3	theme	enhanced	981:988	arg1	Tg					1020:1021	Tg	1020:1021	Tg	1020:1021	DSC showed enhanced glass transition temperature (Tg) due to the interfacial interactions between membrane constituents which restrict the mobility of polymer chains.
32896564	7	4	theme	higher	1166:1171	arg1	roughness					1181:1189	higher surface roughness	1166:1189	higher surface roughness of composites	1166:1203	Microscopic imaging revealed higher surface roughness of composites compared to neat PES.
32896564	3	5	theme	differential	564:575	arg1	DSC					599:601	DSC	599:601	DSC	599:601	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	3	5	theme	differential	564:575	arg1	calorimetry					586:596	differential scanning calorimetry	564:596	differential scanning calorimetry (DSC)	564:602	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	4	6	attach	presence	778:785	arg1	matrix					807:812	the host matrix	798:812	the host matrix	798:812	The presence of MMCC in the host matrix was confirmed by FTIR.
32896564	4	6	attach	presence	778:785	arg2	MMCC					790:793	MMCC	790:793	MMCC	790:793	The presence of MMCC in the host matrix was confirmed by FTIR.
32896564	2	7	theme	modified	334:341	arg1	MMCC					348:351	MMCC	348:351	MMCC	348:351	Polyethersulfone (PES) membranes were incorporated with different concentrations of modified MCC (MMCC) to enhance its affinity for heavy metals during filtration of aqueous solutions.
32896564	2	7	theme	modified	334:341	arg1	MCC					343:345	modified MCC	334:345	modified MCC (MMCC)	334:352	Polyethersulfone (PES) membranes were incorporated with different concentrations of modified MCC (MMCC) to enhance its affinity for heavy metals during filtration of aqueous solutions.
32896564	5	8	theme	performance	944:954	arg1	requirements					956:967	their performance requirements	938:967	their performance requirements	938:967	Although composites decomposed at lower temperatures, their thermal stability was sufficient to meet their performance requirements.
32896564	3	9	theme	infrared	500:507	arg1	spectroscopy					509:520	infrared spectroscopy	500:520	infrared spectroscopy (FTIR)	500:527	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	3	9	theme	infrared	500:507	arg1	FTIR					523:526	FTIR	523:526	FTIR	523:526	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	7	10	theme	composites	1194:1203	arg1	roughness					1181:1189	higher surface roughness	1166:1189	higher surface roughness of composites	1166:1203	Microscopic imaging revealed higher surface roughness of composites compared to neat PES.
32896564	6	11	theme	membrane	1068:1075	arg1	constituents					1077:1088	membrane constituents	1068:1088	membrane constituents which restrict the mobility of polymer chains	1068:1134	DSC showed enhanced glass transition temperature (Tg) due to the interfacial interactions between membrane constituents which restrict the mobility of polymer chains.
32896564	3	12	theme	composite	439:447	arg1	membranes					449:457	The composite membranes	435:457	The composite membranes	435:457	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	7	13	theme	Microscopic	1137:1147	arg1	imaging					1149:1155	Microscopic imaging	1137:1155	Microscopic imaging	1137:1155	Microscopic imaging revealed higher surface roughness of composites compared to neat PES.
32896564	3	14	theme	BET	696:698	arg1	method					701:706	Brunauer-Emmett-Teller (BET) method	672:706	Brunauer-Emmett-Teller (BET) method	672:706	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	1	15	theme	3-chloropropyl	190:203	arg1	agent					243:247	a coupling agent	232:247	a coupling agent	232:247	In this investigation, microcrystalline cellulose (MCC) was functionalized with metformin HCl using (3-chloropropyl)triethixysilane (CPTES) as a coupling agent.
32896564	1	15	theme	3-chloropropyl	190:203	arg1	CPTES					222:226	CPTES	222:226	CPTES	222:226	In this investigation, microcrystalline cellulose (MCC) was functionalized with metformin HCl using (3-chloropropyl)triethixysilane (CPTES) as a coupling agent.
32896564	1	15	theme	3-chloropropyl	190:203	arg1	triethixysilane					205:219	(3-chloropropyl)triethixysilane	189:219	(3-chloropropyl)triethixysilane (CPTES)	189:227	In this investigation, microcrystalline cellulose (MCC) was functionalized with metformin HCl using (3-chloropropyl)triethixysilane (CPTES) as a coupling agent.
32896564	3	16	theme	thermogravimetric	530:546	arg1	TGA					558:560	TGA	558:560	TGA	558:560	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	3	16	theme	thermogravimetric	530:546	arg1	analysis					548:555	thermogravimetric analysis	530:555	thermogravimetric analysis (TGA)	530:561	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	3	17	theme	scanning	577:584	arg1	DSC					599:601	DSC	599:601	DSC	599:601	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	3	17	theme	scanning	577:584	arg1	calorimetry					586:596	differential scanning calorimetry	564:596	differential scanning calorimetry (DSC)	564:602	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	2	18	theme	heavy	382:386	arg1	metals					388:393	heavy metals	382:393	heavy metals	382:393	Polyethersulfone (PES) membranes were incorporated with different concentrations of modified MCC (MMCC) to enhance its affinity for heavy metals during filtration of aqueous solutions.
32896564	3	19	theme	contact	722:728	arg1	measurements					736:747	contact angle measurements	722:747	contact angle measurements	722:747	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	1	20	theme	microcrystalline	112:127	arg1	MCC					140:142	MCC	140:142	MCC	140:142	In this investigation, microcrystalline cellulose (MCC) was functionalized with metformin HCl using (3-chloropropyl)triethixysilane (CPTES) as a coupling agent.
32896564	1	20	theme	microcrystalline	112:127	arg1	cellulose					129:137	microcrystalline cellulose	112:137	microcrystalline cellulose (MCC)	112:143	In this investigation, microcrystalline cellulose (MCC) was functionalized with metformin HCl using (3-chloropropyl)triethixysilane (CPTES) as a coupling agent.
32896564	6	21	theme	interfacial	1035:1045	arg1	interactions					1047:1058	the interfacial interactions	1031:1058	the interfacial interactions between membrane constituents which restrict the mobility of polymer chains	1031:1134	DSC showed enhanced glass transition temperature (Tg) due to the interfacial interactions between membrane constituents which restrict the mobility of polymer chains.
32896564	7	22	theme	neat	1217:1220	arg1	PES					1222:1224	neat PES	1217:1224	neat PES	1217:1224	Microscopic imaging revealed higher surface roughness of composites compared to neat PES.
32896564	3	23	theme	scanning	605:612	arg1	SEM					635:637	SEM	635:637	SEM	635:637	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	3	23	theme	scanning	605:612	arg1	microscopy					623:632	scanning electron microscopy	605:632	scanning electron microscopy (SEM)	605:638	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	2	24	theme	solutions	424:432	arg1	filtration					402:411	filtration	402:411	filtration of aqueous solutions	402:432	Polyethersulfone (PES) membranes were incorporated with different concentrations of modified MCC (MMCC) to enhance its affinity for heavy metals during filtration of aqueous solutions.
32896564	7	25	theme	surface	1173:1179	arg1	roughness					1181:1189	higher surface roughness	1166:1189	higher surface roughness of composites	1166:1203	Microscopic imaging revealed higher surface roughness of composites compared to neat PES.
32896564	2	26	theme	different	306:314	arg1	concentrations					316:329	different concentrations	306:329	different concentrations of modified MCC (MMCC)	306:352	Polyethersulfone (PES) membranes were incorporated with different concentrations of modified MCC (MMCC) to enhance its affinity for heavy metals during filtration of aqueous solutions.
32896564	2	27	theme	aqueous	416:422	arg1	solutions					424:432	aqueous solutions	416:432	aqueous solutions	416:432	Polyethersulfone (PES) membranes were incorporated with different concentrations of modified MCC (MMCC) to enhance its affinity for heavy metals during filtration of aqueous solutions.
32896564	0	28	theme	functionalized	46:59	arg1	cellulose					78:86	amine functionalized microcrystalline cellulose	40:86	amine functionalized microcrystalline cellulose	40:86	Polyethersulfone membrane embedded with amine functionalized microcrystalline cellulose.
32896564	8	29	theme	membrane	1294:1301	arg1	hydrophilicity					1272:1285	hydrophilicity	1272:1285	hydrophilicity	1272:1285	Inclusion of MMCC increased the porosity and hydrophilicity of the membrane which consequently, higher permeability can be achieved.
32896564	8	29	theme	membrane	1294:1301	arg1	porosity					1259:1266	porosity	1259:1266	porosity	1259:1266	Inclusion of MMCC increased the porosity and hydrophilicity of the membrane which consequently, higher permeability can be achieved.
32896564	3	30	theme	atomic	641:646	arg1	microscopy					654:663	atomic force microscopy	641:663	atomic force microscopy (AFM)	641:669	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	3	30	theme	atomic	641:646	arg1	AFM					666:668	AFM	666:668	AFM	666:668	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	0	31	theme	amine	40:44	arg1	cellulose					78:86	amine functionalized microcrystalline cellulose	40:86	amine functionalized microcrystalline cellulose	40:86	Polyethersulfone membrane embedded with amine functionalized microcrystalline cellulose.
32896564	3	32	theme	mechanical	753:762	arg1	analysis					764:771	mechanical analysis	753:771	mechanical analysis	753:771	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	4	33	theme	MMCC	790:793	arg1	presence					778:785	The presence	774:785	The presence of MMCC in the host matrix	774:812	The presence of MMCC in the host matrix was confirmed by FTIR.
32896564	3	34	theme	angle	730:734	arg1	measurements					736:747	contact angle measurements	722:747	contact angle measurements	722:747	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	4	35	theme	host	802:805	arg1	matrix					807:812	the host matrix	798:812	the host matrix	798:812	The presence of MMCC in the host matrix was confirmed by FTIR.
32896564	6	36	theme	chains	1129:1134	arg1	mobility					1109:1116	the mobility	1105:1116	the mobility of polymer chains	1105:1134	DSC showed enhanced glass transition temperature (Tg) due to the interfacial interactions between membrane constituents which restrict the mobility of polymer chains.
32896564	0	37	theme	microcrystalline	61:76	arg1	cellulose					78:86	amine functionalized microcrystalline cellulose	40:86	amine functionalized microcrystalline cellulose	40:86	Polyethersulfone membrane embedded with amine functionalized microcrystalline cellulose.
32896564	0	38	with	membrane	17:24	arg1	cellulose					78:86	amine functionalized microcrystalline cellulose	40:86	amine functionalized microcrystalline cellulose	40:86	Polyethersulfone membrane embedded with amine functionalized microcrystalline cellulose.
32896564	6	39	theme	polymer	1121:1127	arg1	chains					1129:1134	polymer chains	1121:1134	polymer chains	1121:1134	DSC showed enhanced glass transition temperature (Tg) due to the interfacial interactions between membrane constituents which restrict the mobility of polymer chains.
32896564	3	40	theme	electron	614:621	arg1	SEM					635:637	SEM	635:637	SEM	635:637	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	3	40	theme	electron	614:621	arg1	microscopy					623:632	scanning electron microscopy	605:632	scanning electron microscopy (SEM)	605:638	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	3	41	dep	fourier	482:488	arg1	transform					490:498	transform	490:498	transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis	490:771	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	5	42	theme	thermal	897:903	arg1	stability					905:913	their thermal stability	891:913	their thermal stability	891:913	Although composites decomposed at lower temperatures, their thermal stability was sufficient to meet their performance requirements.
32896564	3	43	theme	force	648:652	arg1	microscopy					654:663	atomic force microscopy	641:663	atomic force microscopy (AFM)	641:669	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	3	43	theme	force	648:652	arg1	AFM					666:668	AFM	666:668	AFM	666:668	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	2	44	theme	MCC	343:345	arg1	concentrations					316:329	different concentrations	306:329	different concentrations of modified MCC (MMCC)	306:352	Polyethersulfone (PES) membranes were incorporated with different concentrations of modified MCC (MMCC) to enhance its affinity for heavy metals during filtration of aqueous solutions.
32896564	1	45	theme	coupling	234:241	arg1	agent					243:247	a coupling agent	232:247	a coupling agent	232:247	In this investigation, microcrystalline cellulose (MCC) was functionalized with metformin HCl using (3-chloropropyl)triethixysilane (CPTES) as a coupling agent.
32896564	1	45	theme	coupling	234:241	arg1	triethixysilane					205:219	(3-chloropropyl)triethixysilane	189:219	(3-chloropropyl)triethixysilane (CPTES)	189:227	In this investigation, microcrystalline cellulose (MCC) was functionalized with metformin HCl using (3-chloropropyl)triethixysilane (CPTES) as a coupling agent.
32896564	0	46	dep	membrane	17:24	arg1	embedded					26:33	embedded	26:33	embedded	26:33	Polyethersulfone membrane embedded with amine functionalized microcrystalline cellulose.
32896564	4	47	from	presence	778:785	arg1	matrix					807:812	the host matrix	798:812	the host matrix	798:812	The presence of MMCC in the host matrix was confirmed by FTIR.
32896564	8	48	theme	MMCC	1240:1243	arg1	Inclusion					1227:1235	Inclusion	1227:1235	Inclusion of MMCC	1227:1243	Inclusion of MMCC increased the porosity and hydrophilicity of the membrane which consequently, higher permeability can be achieved.
32896564	3	49	theme	Brunauer-Emmett-Teller	672:693	arg1	method					701:706	Brunauer-Emmett-Teller (BET) method	672:706	Brunauer-Emmett-Teller (BET) method	672:706	The composite membranes were characterized via fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), Brunauer-Emmett-Teller (BET) method, porosity and contact angle measurements and mechanical analysis.
32896564	2	50	theme	Polyethersulfone	250:265	arg1	membranes					273:281	Polyethersulfone (PES) membranes	250:281	Polyethersulfone (PES) membranes	250:281	Polyethersulfone (PES) membranes were incorporated with different concentrations of modified MCC (MMCC) to enhance its affinity for heavy metals during filtration of aqueous solutions.
32896564	1	51	theme	metformin	169:177	arg1	HCl					179:181	metformin HCl	169:181	metformin HCl using (3-chloropropyl)triethixysilane (CPTES) as a coupling agent	169:247	In this investigation, microcrystalline cellulose (MCC) was functionalized with metformin HCl using (3-chloropropyl)triethixysilane (CPTES) as a coupling agent.
32896564	6	52	theme	transition	996:1005	arg1	temperature					1007:1017	enhanced glass transition temperature	981:1017	enhanced glass transition temperature (Tg)	981:1022	DSC showed enhanced glass transition temperature (Tg) due to the interfacial interactions between membrane constituents which restrict the mobility of polymer chains.
32896564	6	52	theme	transition	996:1005	arg1	Tg					1020:1021	Tg	1020:1021	Tg	1020:1021	DSC showed enhanced glass transition temperature (Tg) due to the interfacial interactions between membrane constituents which restrict the mobility of polymer chains.
33587924	2	0	theme	As	458:459	arg1	removal					468:474	As(III/V) removal	458:474	As(III/V) removal from aqueous solution	458:496	This present study focused on a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation and its performance for As(III/V) removal from aqueous solution.
33587924	8	1	theme	low	1458:1460	arg1	concentration					1441:1453	a residue concentration	1431:1453	a residue concentration as low as the 10 μg L-1 WHO guideline for drinking water	1431:1510	Note that the employment of Zr-CTS/SA in low-concentration arsenic solution exhibited a residue concentration as low as the 10 μg L-1 WHO guideline for drinking water.
33587924	4	2	theme	pristine	806:813	arg1	beads					818:822	pristine SA beads	806:822	pristine SA beads	806:822	Experimental data showed that Zr-CTS/SA had considerable adsorbability for As(III) and As(V), the adsorption capacities were enhanced about at least 20 and 6 times separately compared with pristine SA beads.
33587924	2	3	theme	present	311:317	arg1	study					319:323	This present study	306:323	This present study	306:323	This present study focused on a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation and its performance for As(III/V) removal from aqueous solution.
33587924	2	4	dep	As	458:459	arg1	III/V					461:465	III/V	461:465	III/V	461:465	This present study focused on a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation and its performance for As(III/V) removal from aqueous solution.
33587924	4	5	contain	had	657:659	arg2	adsorbability					674:686	considerable adsorbability	661:686	considerable adsorbability for As(III) and As(V)	661:708	Experimental data showed that Zr-CTS/SA had considerable adsorbability for As(III) and As(V), the adsorption capacities were enhanced about at least 20 and 6 times separately compared with pristine SA beads.
33587924	4	5	contain	had	657:659	arg1	Zr-CTS/SA					647:655	Zr-CTS/SA	647:655	Zr-CTS/SA	647:655	Experimental data showed that Zr-CTS/SA had considerable adsorbability for As(III) and As(V), the adsorption capacities were enhanced about at least 20 and 6 times separately compared with pristine SA beads.
33587924	5	6	theme	adsorption	829:838	arg1	processes					840:848	The adsorption processes	825:848	The adsorption processes of As(III) and As(V)	825:869	The adsorption processes of As(III) and As(V) could both be described with Langmuir isotherm model and the maximum adsorption capacities reached 43.19 and 76.78 mg g-1, respectively.
33587924	2	7	theme	aqueous	481:487	arg1	solution					489:496	aqueous solution	481:496	aqueous solution	481:496	This present study focused on a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation and its performance for As(III/V) removal from aqueous solution.
33587924	4	8	theme	SA	815:816	arg1	beads					818:822	pristine SA beads	806:822	pristine SA beads	806:822	Experimental data showed that Zr-CTS/SA had considerable adsorbability for As(III) and As(V), the adsorption capacities were enhanced about at least 20 and 6 times separately compared with pristine SA beads.
33587924	3	9	theme	obtained	503:510	arg1	composite					512:520	The obtained composite	499:520	The obtained composite	499:520	The obtained composite presented a spherical structure with a diameter of 2.0-3.0 mm and favorable thermal stability.
33587924	7	10	theme	protonated	1313:1322	arg1	groups					1337:1342	protonated -OH and -NH2 groups	1313:1342	protonated -OH and -NH2 groups	1313:1342	Moreover, the adsorption mechanisms of As(III/V) involved ligand exchange with Cl on the surface of Zr-CTS/SA, another reaction pathway for As(V) was the electrostatic attraction with protonated -OH and -NH2 groups.
33587924	7	11	theme	reaction	1248:1255	arg1	pathway					1257:1263	another reaction pathway	1240:1263	another reaction pathway for As(V) was the electrostatic attraction with protonated -OH and -NH2 groups	1240:1342	Moreover, the adsorption mechanisms of As(III/V) involved ligand exchange with Cl on the surface of Zr-CTS/SA, another reaction pathway for As(V) was the electrostatic attraction with protonated -OH and -NH2 groups.
33587924	7	11	theme	reaction	1248:1255	arg1	Zr-CTS/SA					1229:1237	Zr-CTS/SA	1229:1237	Zr-CTS/SA	1229:1237	Moreover, the adsorption mechanisms of As(III/V) involved ligand exchange with Cl on the surface of Zr-CTS/SA, another reaction pathway for As(V) was the electrostatic attraction with protonated -OH and -NH2 groups.
33587924	4	12	theme	adsorption	715:724	arg1	capacities					726:735	the adsorption capacities	711:735	the adsorption capacities	711:735	Experimental data showed that Zr-CTS/SA had considerable adsorbability for As(III) and As(V), the adsorption capacities were enhanced about at least 20 and 6 times separately compared with pristine SA beads.
33587924	1	13	theme	Most	125:128	arg1	adsorbents					142:151	Most nano-scaled adsorbents	125:151	Most nano-scaled adsorbents	125:151	Most nano-scaled adsorbents have trouble in separating from aqueous solution, thus, a need for new materials of facile separation and predominant adsorption performance has arisen.
33587924	7	14	theme	electrostatic	1283:1295	arg1	attraction					1297:1306	the electrostatic attraction	1279:1306	the electrostatic attraction with protonated -OH and -NH2 groups	1279:1342	Moreover, the adsorption mechanisms of As(III/V) involved ligand exchange with Cl on the surface of Zr-CTS/SA, another reaction pathway for As(V) was the electrostatic attraction with protonated -OH and -NH2 groups.
33587924	6	15	theme	intra-particle	1049:1062	arg1	model					1074:1078	the intra-particle diffusion model	1045:1078	the intra-particle diffusion model	1045:1078	The kinetic data of As(III) followed the intra-particle diffusion model while As(V) fitted the pseudo-first-order model.
33587924	1	16	theme	nano-scaled	130:140	arg1	adsorbents					142:151	Most nano-scaled adsorbents	125:151	Most nano-scaled adsorbents	125:151	Most nano-scaled adsorbents have trouble in separating from aqueous solution, thus, a need for new materials of facile separation and predominant adsorption performance has arisen.
33587924	2	17	theme	composite	412:420	arg1	preparation					422:432	a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation	336:432	a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation	336:432	This present study focused on a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation and its performance for As(III/V) removal from aqueous solution.
33587924	8	18	theme	Zr-CTS/SA	1373:1381	arg1	employment					1359:1368	the employment	1355:1368	the employment of Zr-CTS/SA in low-concentration arsenic solution	1355:1419	Note that the employment of Zr-CTS/SA in low-concentration arsenic solution exhibited a residue concentration as low as the 10 μg L-1 WHO guideline for drinking water.
33587924	7	19	theme	-NH2	1332:1335	arg1	groups					1337:1342	protonated -OH and -NH2 groups	1313:1342	protonated -OH and -NH2 groups	1313:1342	Moreover, the adsorption mechanisms of As(III/V) involved ligand exchange with Cl on the surface of Zr-CTS/SA, another reaction pathway for As(V) was the electrostatic attraction with protonated -OH and -NH2 groups.
33587924	8	20	theme	arsenic	1404:1410	arg1	solution					1412:1419	low-concentration arsenic solution	1386:1419	low-concentration arsenic solution	1386:1419	Note that the employment of Zr-CTS/SA in low-concentration arsenic solution exhibited a residue concentration as low as the 10 μg L-1 WHO guideline for drinking water.
33587924	7	21	with	exchange	1194:1201	arg1	Cl					1208:1209	Cl	1208:1209	Cl	1208:1209	Moreover, the adsorption mechanisms of As(III/V) involved ligand exchange with Cl on the surface of Zr-CTS/SA, another reaction pathway for As(V) was the electrostatic attraction with protonated -OH and -NH2 groups.
33587924	7	22	theme	ligand	1187:1192	arg1	exchange					1194:1201	ligand exchange	1187:1201	ligand exchange with Cl on the surface of Zr-CTS/SA, another reaction pathway for As(V) was the electrostatic attraction with protonated -OH and -NH2 groups	1187:1342	Moreover, the adsorption mechanisms of As(III/V) involved ligand exchange with Cl on the surface of Zr-CTS/SA, another reaction pathway for As(V) was the electrostatic attraction with protonated -OH and -NH2 groups.
33587924	0	23	theme	Enhanced	0:7	arg1	removal					9:15	Enhanced removal	0:15	Enhanced removal of As(III) and As(V) from water by a novel zirconium-chitosan	0:77	Enhanced removal of As(III) and As(V) from water by a novel zirconium-chitosan modified spherical sodium alginate composite.
33587924	1	24	theme	new	220:222	arg1	materials					224:232	new materials	220:232	new materials of facile separation and predominant adsorption performance	220:292	Most nano-scaled adsorbents have trouble in separating from aqueous solution, thus, a need for new materials of facile separation and predominant adsorption performance has arisen.
33587924	3	25	theme	spherical	534:542	arg1	structure					544:552	a spherical structure	532:552	a spherical structure	532:552	The obtained composite presented a spherical structure with a diameter of 2.0-3.0 mm and favorable thermal stability.
33587924	0	26	theme	As	20:21	arg1	removal					9:15	Enhanced removal	0:15	Enhanced removal of As(III) and As(V) from water by a novel zirconium-chitosan	0:77	Enhanced removal of As(III) and As(V) from water by a novel zirconium-chitosan modified spherical sodium alginate composite.
33587924	8	27	theme	low-concentration	1386:1402	arg1	solution					1412:1419	low-concentration arsenic solution	1386:1419	low-concentration arsenic solution	1386:1419	Note that the employment of Zr-CTS/SA in low-concentration arsenic solution exhibited a residue concentration as low as the 10 μg L-1 WHO guideline for drinking water.
33587924	5	28	theme	As	865:866	arg1	processes					840:848	The adsorption processes	825:848	The adsorption processes of As(III) and As(V)	825:869	The adsorption processes of As(III) and As(V) could both be described with Langmuir isotherm model and the maximum adsorption capacities reached 43.19 and 76.78 mg g-1, respectively.
33587924	5	29	theme	isotherm	909:916	arg1	model					918:922	Langmuir isotherm model	900:922	Langmuir isotherm model	900:922	The adsorption processes of As(III) and As(V) could both be described with Langmuir isotherm model and the maximum adsorption capacities reached 43.19 and 76.78 mg g-1, respectively.
33587924	2	30	theme	alginate	391:398	arg1	preparation					422:432	a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation	336:432	a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation	336:432	This present study focused on a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation and its performance for As(III/V) removal from aqueous solution.
33587924	0	31	theme	novel	54:58	arg1	zirconium-chitosan					60:77	a novel zirconium-chitosan	52:77	a novel zirconium-chitosan	52:77	Enhanced removal of As(III) and As(V) from water by a novel zirconium-chitosan modified spherical sodium alginate composite.
33587924	1	32	contain	have	153:156	arg1	adsorbents					142:151	Most nano-scaled adsorbents	125:151	Most nano-scaled adsorbents	125:151	Most nano-scaled adsorbents have trouble in separating from aqueous solution, thus, a need for new materials of facile separation and predominant adsorption performance has arisen.
33587924	1	32	contain	have	153:156	arg2	trouble					158:164	trouble	158:164	trouble	158:164	Most nano-scaled adsorbents have trouble in separating from aqueous solution, thus, a need for new materials of facile separation and predominant adsorption performance has arisen.
33587924	6	33	theme	III	1031:1033	arg1	data					1020:1023	The kinetic data	1008:1023	The kinetic data of As(III)	1008:1034	The kinetic data of As(III) followed the intra-particle diffusion model while As(V) fitted the pseudo-first-order model.
33587924	2	34	theme	sodium	384:389	arg1	preparation					422:432	a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation	336:432	a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation	336:432	This present study focused on a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation and its performance for As(III/V) removal from aqueous solution.
33587924	1	35	theme	facile	237:242	arg1	separation					244:253	facile separation	237:253	facile separation	237:253	Most nano-scaled adsorbents have trouble in separating from aqueous solution, thus, a need for new materials of facile separation and predominant adsorption performance has arisen.
33587924	0	36	theme	spherical	88:96	arg1	composite					114:122	spherical sodium alginate composite	88:122	spherical sodium alginate composite	88:122	Enhanced removal of As(III) and As(V) from water by a novel zirconium-chitosan modified spherical sodium alginate composite.
33587924	4	37	theme	Experimental	617:628	arg1	data					630:633	Experimental data	617:633	Experimental data	617:633	Experimental data showed that Zr-CTS/SA had considerable adsorbability for As(III) and As(V), the adsorption capacities were enhanced about at least 20 and 6 times separately compared with pristine SA beads.
33587924	5	38	theme	Langmuir	900:907	arg1	model					918:922	Langmuir isotherm model	900:922	Langmuir isotherm model	900:922	The adsorption processes of As(III) and As(V) could both be described with Langmuir isotherm model and the maximum adsorption capacities reached 43.19 and 76.78 mg g-1, respectively.
33587924	2	39	theme	modified	375:382	arg1	preparation					422:432	a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation	336:432	a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation	336:432	This present study focused on a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation and its performance for As(III/V) removal from aqueous solution.
33587924	2	40	theme	segregative	344:354	arg1	preparation					422:432	a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation	336:432	a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation	336:432	This present study focused on a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation and its performance for As(III/V) removal from aqueous solution.
33587924	1	41	theme	separation	244:253	arg1	materials					224:232	new materials	220:232	new materials of facile separation and predominant adsorption performance	220:292	Most nano-scaled adsorbents have trouble in separating from aqueous solution, thus, a need for new materials of facile separation and predominant adsorption performance has arisen.
33587924	3	42	theme	thermal	598:604	arg1	stability					606:614	favorable thermal stability	588:614	favorable thermal stability	588:614	The obtained composite presented a spherical structure with a diameter of 2.0-3.0 mm and favorable thermal stability.
33587924	2	43	theme	Zr-CTS/SA	401:409	arg1	preparation					422:432	a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation	336:432	a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation	336:432	This present study focused on a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation and its performance for As(III/V) removal from aqueous solution.
33587924	2	44	theme	zirconium-chitosan	356:373	arg1	preparation					422:432	a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation	336:432	a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation	336:432	This present study focused on a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation and its performance for As(III/V) removal from aqueous solution.
33587924	7	45	dep	attraction	1297:1306	arg1	V					1272:1272	V	1272:1272	V	1272:1272	Moreover, the adsorption mechanisms of As(III/V) involved ligand exchange with Cl on the surface of Zr-CTS/SA, another reaction pathway for As(V) was the electrostatic attraction with protonated -OH and -NH2 groups.
33587924	0	46	theme	alginate	105:112	arg1	composite					114:122	spherical sodium alginate composite	88:122	spherical sodium alginate composite	88:122	Enhanced removal of As(III) and As(V) from water by a novel zirconium-chitosan modified spherical sodium alginate composite.
33587924	3	47	theme	stability	606:614	arg1	diameter					561:568	a diameter	559:568	a diameter of 2.0-3.0 mm and favorable thermal stability	559:614	The obtained composite presented a spherical structure with a diameter of 2.0-3.0 mm and favorable thermal stability.
33587924	7	48	theme	Zr-CTS/SA	1229:1237	arg1	surface					1218:1224	the surface	1214:1224	the surface of Zr-CTS/SA, another reaction pathway for As(V) was the electrostatic attraction with protonated -OH and -NH2 groups	1214:1342	Moreover, the adsorption mechanisms of As(III/V) involved ligand exchange with Cl on the surface of Zr-CTS/SA, another reaction pathway for As(V) was the electrostatic attraction with protonated -OH and -NH2 groups.
33587924	6	49	theme	kinetic	1012:1018	arg1	data					1020:1023	The kinetic data	1008:1023	The kinetic data of As(III)	1008:1034	The kinetic data of As(III) followed the intra-particle diffusion model while As(V) fitted the pseudo-first-order model.
33587924	7	50	dep	As	1269:1270	arg1	attraction					1297:1306	the electrostatic attraction	1279:1306	the electrostatic attraction with protonated -OH and -NH2 groups	1279:1342	Moreover, the adsorption mechanisms of As(III/V) involved ligand exchange with Cl on the surface of Zr-CTS/SA, another reaction pathway for As(V) was the electrostatic attraction with protonated -OH and -NH2 groups.
33587924	8	51	from	employment	1359:1368	arg1	solution					1412:1419	low-concentration arsenic solution	1386:1419	low-concentration arsenic solution	1386:1419	Note that the employment of Zr-CTS/SA in low-concentration arsenic solution exhibited a residue concentration as low as the 10 μg L-1 WHO guideline for drinking water.
33587924	1	52	theme	predominant	259:269	arg1	performance					282:292	predominant adsorption performance	259:292	predominant adsorption performance	259:292	Most nano-scaled adsorbents have trouble in separating from aqueous solution, thus, a need for new materials of facile separation and predominant adsorption performance has arisen.
33587924	0	53	theme	sodium	98:103	arg1	composite					114:122	spherical sodium alginate composite	88:122	spherical sodium alginate composite	88:122	Enhanced removal of As(III) and As(V) from water by a novel zirconium-chitosan modified spherical sodium alginate composite.
33587924	5	54	theme	maximum	932:938	arg1	capacities					951:960	the maximum adsorption capacities	928:960	the maximum adsorption capacities reached 43.19 and 76.78 mg g-1, respectively	928:1005	The adsorption processes of As(III) and As(V) could both be described with Langmuir isotherm model and the maximum adsorption capacities reached 43.19 and 76.78 mg g-1, respectively.
33587924	5	55	theme	As	853:854	arg1	processes					840:848	The adsorption processes	825:848	The adsorption processes of As(III) and As(V)	825:869	The adsorption processes of As(III) and As(V) could both be described with Langmuir isotherm model and the maximum adsorption capacities reached 43.19 and 76.78 mg g-1, respectively.
33587924	1	56	theme	adsorption	271:280	arg1	performance					282:292	predominant adsorption performance	259:292	predominant adsorption performance	259:292	Most nano-scaled adsorbents have trouble in separating from aqueous solution, thus, a need for new materials of facile separation and predominant adsorption performance has arisen.
33587924	5	57	theme	adsorption	940:949	arg1	capacities					951:960	the maximum adsorption capacities	928:960	the maximum adsorption capacities reached 43.19 and 76.78 mg g-1, respectively	928:1005	The adsorption processes of As(III) and As(V) could both be described with Langmuir isotherm model and the maximum adsorption capacities reached 43.19 and 76.78 mg g-1, respectively.
33587924	6	58	theme	pseudo-first-order	1103:1120	arg1	model					1122:1126	the pseudo-first-order model	1099:1126	the pseudo-first-order model	1099:1126	The kinetic data of As(III) followed the intra-particle diffusion model while As(V) fitted the pseudo-first-order model.
33587924	7	59	theme	adsorption	1143:1152	arg1	mechanisms					1154:1163	the adsorption mechanisms	1139:1163	the adsorption mechanisms of As(III/V)	1139:1176	Moreover, the adsorption mechanisms of As(III/V) involved ligand exchange with Cl on the surface of Zr-CTS/SA, another reaction pathway for As(V) was the electrostatic attraction with protonated -OH and -NH2 groups.
33587924	7	60	with	attraction	1297:1306	arg1	groups					1337:1342	protonated -OH and -NH2 groups	1313:1342	protonated -OH and -NH2 groups	1313:1342	Moreover, the adsorption mechanisms of As(III/V) involved ligand exchange with Cl on the surface of Zr-CTS/SA, another reaction pathway for As(V) was the electrostatic attraction with protonated -OH and -NH2 groups.
33587924	1	61	theme	aqueous	185:191	arg1	solution					193:200	aqueous solution	185:200	aqueous solution	185:200	Most nano-scaled adsorbents have trouble in separating from aqueous solution, thus, a need for new materials of facile separation and predominant adsorption performance has arisen.
33587924	0	62	from	water	43:47	arg1	removal					9:15	Enhanced removal	0:15	Enhanced removal of As(III) and As(V) from water by a novel zirconium-chitosan	0:77	Enhanced removal of As(III) and As(V) from water by a novel zirconium-chitosan modified spherical sodium alginate composite.
33587924	3	63	theme	favorable	588:596	arg1	stability					606:614	favorable thermal stability	588:614	favorable thermal stability	588:614	The obtained composite presented a spherical structure with a diameter of 2.0-3.0 mm and favorable thermal stability.
33587924	6	64	theme	diffusion	1064:1072	arg1	model					1074:1078	the intra-particle diffusion model	1045:1078	the intra-particle diffusion model	1045:1078	The kinetic data of As(III) followed the intra-particle diffusion model while As(V) fitted the pseudo-first-order model.
33587924	8	65	theme	residue	1433:1439	arg1	concentration					1441:1453	a residue concentration	1431:1453	a residue concentration as low as the 10 μg L-1 WHO guideline for drinking water	1431:1510	Note that the employment of Zr-CTS/SA in low-concentration arsenic solution exhibited a residue concentration as low as the 10 μg L-1 WHO guideline for drinking water.
33587924	2	66	from	solution	489:496	arg1	removal					468:474	As(III/V) removal	458:474	As(III/V) removal from aqueous solution	458:496	This present study focused on a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation and its performance for As(III/V) removal from aqueous solution.
33587924	7	67	from	exchange	1194:1201	arg1	surface					1218:1224	the surface	1214:1224	the surface of Zr-CTS/SA, another reaction pathway for As(V) was the electrostatic attraction with protonated -OH and -NH2 groups	1214:1342	Moreover, the adsorption mechanisms of As(III/V) involved ligand exchange with Cl on the surface of Zr-CTS/SA, another reaction pathway for As(V) was the electrostatic attraction with protonated -OH and -NH2 groups.
33587924	4	68	theme	considerable	661:672	arg1	adsorbability					674:686	considerable adsorbability	661:686	considerable adsorbability for As(III) and As(V)	661:708	Experimental data showed that Zr-CTS/SA had considerable adsorbability for As(III) and As(V), the adsorption capacities were enhanced about at least 20 and 6 times separately compared with pristine SA beads.
33587924	2	69	theme	novel	338:342	arg1	preparation					422:432	a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation	336:432	a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation	336:432	This present study focused on a novel segregative zirconium-chitosan modified sodium alginate (Zr-CTS/SA) composite preparation and its performance for As(III/V) removal from aqueous solution.
33587924	7	70	theme	-OH	1324:1326	arg1	groups					1337:1342	protonated -OH and -NH2 groups	1313:1342	protonated -OH and -NH2 groups	1313:1342	Moreover, the adsorption mechanisms of As(III/V) involved ligand exchange with Cl on the surface of Zr-CTS/SA, another reaction pathway for As(V) was the electrostatic attraction with protonated -OH and -NH2 groups.
33587924	0	71	theme	As	32:33	arg1	removal					9:15	Enhanced removal	0:15	Enhanced removal of As(III) and As(V) from water by a novel zirconium-chitosan	0:77	Enhanced removal of As(III) and As(V) from water by a novel zirconium-chitosan modified spherical sodium alginate composite.
33587924	1	72	theme	performance	282:292	arg1	materials					224:232	new materials	220:232	new materials of facile separation and predominant adsorption performance	220:292	Most nano-scaled adsorbents have trouble in separating from aqueous solution, thus, a need for new materials of facile separation and predominant adsorption performance has arisen.
33587924	3	73	theme	2.0-3.0 mm	573:582	arg1	diameter					561:568	a diameter	559:568	a diameter of 2.0-3.0 mm and favorable thermal stability	559:614	The obtained composite presented a spherical structure with a diameter of 2.0-3.0 mm and favorable thermal stability.
33587924	7	74	theme	As	1168:1169	arg1	mechanisms					1154:1163	the adsorption mechanisms	1139:1163	the adsorption mechanisms of As(III/V)	1139:1176	Moreover, the adsorption mechanisms of As(III/V) involved ligand exchange with Cl on the surface of Zr-CTS/SA, another reaction pathway for As(V) was the electrostatic attraction with protonated -OH and -NH2 groups.
33309657	0	0	theme	engineering	119:129	arg1	application					131:141	tissue engineering application	112:141	tissue engineering application	112:141	Fabrication and characterization of conductive polypyrrole/chitosan/collagen electrospun nanofiber scaffold for tissue engineering application.
33309657	4	1	theme	polypyrrole	740:750	arg1	particles					752:760	The polypyrrole particles	736:760	The polypyrrole particles	736:760	The polypyrrole particles were perfectly dispersed inside the nanofibers, and the fibers average diameter were reducing by increasing the polypyrrole content in the composites.
33309657	1	2	theme	PPy	221:223	arg1	polymer					226:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer	144:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer	144:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer was fabricated to accelerate healing of damaged tissues.
33309657	0	3	theme	tissue	112:117	arg1	engineering					119:129	tissue engineering	112:129	tissue engineering application	112:141	Fabrication and characterization of conductive polypyrrole/chitosan/collagen electrospun nanofiber scaffold for tissue engineering application.
33309657	3	4	theme	in	686:687	arg1	degradation					695:705	in vitro degradation	686:705	in vitro degradation	686:705	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	4	theme	in	686:687	arg1	analyses					603:610	chemical, morphological, physio-mechanical, and biological analyses	544:610	chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture	544:733	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	5	theme	composite	499:507	arg1	scaffolds					509:517	The fabricated composite scaffolds	484:517	The fabricated composite scaffolds	484:517	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	5	6	theme	semi-conductive	1029:1043	arg1	polymers					1060:1067	semi-conductive and conductive polymers	1029:1067	semi-conductive and conductive polymers	1029:1067	The presence of polypyrrole in fibers enhanced their conductivity up to 164.274 × 10-3 s/m which is in the range of semi-conductive and conductive polymers.
33309657	3	7	theme	chemical	544:551	arg1	analyses					603:610	chemical, morphological, physio-mechanical, and biological analyses	544:610	chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture	544:733	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	7	theme	chemical	544:551	arg1	Assay					712:716	MTT Assay	708:716	MTT Assay	708:716	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	7	theme	chemical	544:551	arg1	SEM					642:644	SEM	642:644	SEM	642:644	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	7	theme	chemical	544:551	arg1	conductivity					658:669	electrical conductivity	647:669	electrical conductivity	647:669	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	7	theme	chemical	544:551	arg1	degradation					695:705	in vitro degradation	686:705	in vitro degradation	686:705	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	7	theme	chemical	544:551	arg1	culture					727:733	cell culture	722:733	cell culture	722:733	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	7	theme	chemical	544:551	arg1	spectroscopy					628:639	FTIR spectroscopy	623:639	FTIR spectroscopy	623:639	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	7	theme	chemical	544:551	arg1	test					680:683	tensile test	672:683	tensile test	672:683	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	6	8	theme	cell	1158:1161	arg1	adhesion					1163:1170	better cell adhesion	1151:1170	better cell adhesion	1151:1170	MTT and SEM analyses displayed that nanofibers composing 10% polypyrrole possess better cell adhesion, growth and proliferation properties comparing to other compositions.
33309657	6	9	theme	growth	1173:1178	arg1	properties					1198:1207	better cell adhesion, growth and proliferation properties	1151:1207	better cell adhesion, growth and proliferation properties comparing to other compositions	1151:1239	MTT and SEM analyses displayed that nanofibers composing 10% polypyrrole possess better cell adhesion, growth and proliferation properties comparing to other compositions.
33309657	8	10	theme	heart	1572:1576	arg1	muscles					1578:1584	heart muscles	1572:1584	heart muscles	1572:1584	Therefore, these fabricated conductive nanofiber scaffolds are particularly appropriate for employing in body parts with electrical signals such as cardiovascular, heart muscles, or nerves.
33309657	3	11	theme	morphological	554:566	arg1	analyses					603:610	chemical, morphological, physio-mechanical, and biological analyses	544:610	chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture	544:733	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	11	theme	morphological	554:566	arg1	Assay					712:716	MTT Assay	708:716	MTT Assay	708:716	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	11	theme	morphological	554:566	arg1	SEM					642:644	SEM	642:644	SEM	642:644	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	11	theme	morphological	554:566	arg1	conductivity					658:669	electrical conductivity	647:669	electrical conductivity	647:669	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	11	theme	morphological	554:566	arg1	degradation					695:705	in vitro degradation	686:705	in vitro degradation	686:705	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	11	theme	morphological	554:566	arg1	culture					727:733	cell culture	722:733	cell culture	722:733	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	11	theme	morphological	554:566	arg1	spectroscopy					628:639	FTIR spectroscopy	623:639	FTIR spectroscopy	623:639	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	11	theme	morphological	554:566	arg1	test					680:683	tensile test	672:683	tensile test	672:683	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	2	12	theme	weight	336:341	arg1	percentages					343:353	various weight percentages	328:353	various weight percentages of polypyrrole (5, 10, 15, 20, 25%) relative to the polymers combination (chitosan, collagen, and polyethylene oxide)	328:471	In order to prepare these scaffolds, various weight percentages of polypyrrole (5, 10, 15, 20, 25%) relative to the polymers combination (chitosan, collagen, and polyethylene oxide) were used.
33309657	5	13	theme	polypyrrole	929:939	arg1	presence					917:924	The presence	913:924	The presence of polypyrrole in fibers	913:949	The presence of polypyrrole in fibers enhanced their conductivity up to 164.274 × 10-3 s/m which is in the range of semi-conductive and conductive polymers.
33309657	3	14	theme	cell	722:725	arg1	analyses					603:610	chemical, morphological, physio-mechanical, and biological analyses	544:610	chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture	544:733	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	14	theme	cell	722:725	arg1	culture					727:733	cell culture	722:733	cell culture	722:733	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	7	15	theme	tissue	1346:1351	arg1	skin					1376:1379	skin	1376:1379	skin	1376:1379	Furthermore, the suitable mechanical properties of scaffolds ideally fitted them for different kinds of tissue applications including skin, nerve, heart muscle, etc.
33309657	7	15	theme	tissue	1346:1351	arg1	applications					1353:1364	tissue applications	1346:1364	tissue applications including skin, nerve, heart muscle, etc	1346:1405	Furthermore, the suitable mechanical properties of scaffolds ideally fitted them for different kinds of tissue applications including skin, nerve, heart muscle, etc.
33309657	2	16	theme	various	328:334	arg1	percentages					343:353	various weight percentages	328:353	various weight percentages of polypyrrole (5, 10, 15, 20, 25%) relative to the polymers combination (chitosan, collagen, and polyethylene oxide)	328:471	In order to prepare these scaffolds, various weight percentages of polypyrrole (5, 10, 15, 20, 25%) relative to the polymers combination (chitosan, collagen, and polyethylene oxide) were used.
33309657	6	17	theme	SEM	1078:1080	arg1	analyses					1082:1089	MTT and SEM analyses	1070:1089	MTT and SEM analyses	1070:1089	MTT and SEM analyses displayed that nanofibers composing 10% polypyrrole possess better cell adhesion, growth and proliferation properties comparing to other compositions.
33309657	3	18	theme	MTT	708:710	arg1	analyses					603:610	chemical, morphological, physio-mechanical, and biological analyses	544:610	chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture	544:733	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	18	theme	MTT	708:710	arg1	Assay					712:716	MTT Assay	708:716	MTT Assay	708:716	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	6	19	theme	adhesion	1163:1170	arg1	properties					1198:1207	better cell adhesion, growth and proliferation properties	1151:1207	better cell adhesion, growth and proliferation properties comparing to other compositions	1151:1239	MTT and SEM analyses displayed that nanofibers composing 10% polypyrrole possess better cell adhesion, growth and proliferation properties comparing to other compositions.
33309657	8	20	theme	body	1513:1516	arg1	parts					1518:1522	body parts	1513:1522	body parts with electrical signals such as cardiovascular, heart muscles, or nerves	1513:1595	Therefore, these fabricated conductive nanofiber scaffolds are particularly appropriate for employing in body parts with electrical signals such as cardiovascular, heart muscles, or nerves.
33309657	2	21	theme	relative	391:398	arg1	polypyrrole					358:368	polypyrrole	358:368	polypyrrole (5, 10, 15, 20, 25%) relative to the polymers combination (chitosan, collagen, and polyethylene oxide)	358:471	In order to prepare these scaffolds, various weight percentages of polypyrrole (5, 10, 15, 20, 25%) relative to the polymers combination (chitosan, collagen, and polyethylene oxide) were used.
33309657	2	21	theme	relative	391:398	arg1	%					388:388	5, 10, 15, 20, 25%	371:388	5, 10, 15, 20, 25%	371:388	In order to prepare these scaffolds, various weight percentages of polypyrrole (5, 10, 15, 20, 25%) relative to the polymers combination (chitosan, collagen, and polyethylene oxide) were used.
33309657	7	22	theme	different	1327:1335	arg1	kinds					1337:1341	different kinds	1327:1341	different kinds of tissue applications including skin, nerve, heart muscle, etc	1327:1405	Furthermore, the suitable mechanical properties of scaffolds ideally fitted them for different kinds of tissue applications including skin, nerve, heart muscle, etc.
33309657	3	23	theme	physio-mechanical	569:585	arg1	analyses					603:610	chemical, morphological, physio-mechanical, and biological analyses	544:610	chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture	544:733	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	23	theme	physio-mechanical	569:585	arg1	Assay					712:716	MTT Assay	708:716	MTT Assay	708:716	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	23	theme	physio-mechanical	569:585	arg1	SEM					642:644	SEM	642:644	SEM	642:644	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	23	theme	physio-mechanical	569:585	arg1	conductivity					658:669	electrical conductivity	647:669	electrical conductivity	647:669	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	23	theme	physio-mechanical	569:585	arg1	degradation					695:705	in vitro degradation	686:705	in vitro degradation	686:705	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	23	theme	physio-mechanical	569:585	arg1	culture					727:733	cell culture	722:733	cell culture	722:733	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	23	theme	physio-mechanical	569:585	arg1	spectroscopy					628:639	FTIR spectroscopy	623:639	FTIR spectroscopy	623:639	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	23	theme	physio-mechanical	569:585	arg1	test					680:683	tensile test	672:683	tensile test	672:683	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	24	dep	analyses	603:610	arg1	analyses					603:610	chemical, morphological, physio-mechanical, and biological analyses	544:610	chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture	544:733	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	24	dep	analyses	603:610	arg1	Assay					712:716	MTT Assay	708:716	MTT Assay	708:716	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	24	dep	analyses	603:610	arg1	SEM					642:644	SEM	642:644	SEM	642:644	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	24	dep	analyses	603:610	arg1	conductivity					658:669	electrical conductivity	647:669	electrical conductivity	647:669	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	24	dep	analyses	603:610	arg1	degradation					695:705	in vitro degradation	686:705	in vitro degradation	686:705	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	24	dep	analyses	603:610	arg1	culture					727:733	cell culture	722:733	cell culture	722:733	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	24	dep	analyses	603:610	arg1	spectroscopy					628:639	FTIR spectroscopy	623:639	FTIR spectroscopy	623:639	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	24	dep	analyses	603:610	arg1	test					680:683	tensile test	672:683	tensile test	672:683	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	25	dep	in	686:687	arg1	vitro					689:693	vitro	689:693	vitro	689:693	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	6	26	theme	better	1151:1156	arg1	adhesion					1163:1170	better cell adhesion	1151:1170	better cell adhesion	1151:1170	MTT and SEM analyses displayed that nanofibers composing 10% polypyrrole possess better cell adhesion, growth and proliferation properties comparing to other compositions.
33309657	8	27	with	parts	1518:1522	arg1	signals					1540:1546	electrical signals	1529:1546	electrical signals such as cardiovascular, heart muscles, or nerves	1529:1595	Therefore, these fabricated conductive nanofiber scaffolds are particularly appropriate for employing in body parts with electrical signals such as cardiovascular, heart muscles, or nerves.
33309657	8	27	with	parts	1518:1522	arg1	cardiovascular					1556:1569	cardiovascular	1556:1569	cardiovascular	1556:1569	Therefore, these fabricated conductive nanofiber scaffolds are particularly appropriate for employing in body parts with electrical signals such as cardiovascular, heart muscles, or nerves.
33309657	8	27	with	parts	1518:1522	arg1	muscles					1578:1584	heart muscles	1572:1584	heart muscles	1572:1584	Therefore, these fabricated conductive nanofiber scaffolds are particularly appropriate for employing in body parts with electrical signals such as cardiovascular, heart muscles, or nerves.
33309657	8	27	with	parts	1518:1522	arg1	nerves					1590:1595	nerves	1590:1595	nerves	1590:1595	Therefore, these fabricated conductive nanofiber scaffolds are particularly appropriate for employing in body parts with electrical signals such as cardiovascular, heart muscles, or nerves.
33309657	3	28	theme	biological	592:601	arg1	analyses					603:610	chemical, morphological, physio-mechanical, and biological analyses	544:610	chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture	544:733	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	28	theme	biological	592:601	arg1	Assay					712:716	MTT Assay	708:716	MTT Assay	708:716	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	28	theme	biological	592:601	arg1	SEM					642:644	SEM	642:644	SEM	642:644	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	28	theme	biological	592:601	arg1	conductivity					658:669	electrical conductivity	647:669	electrical conductivity	647:669	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	28	theme	biological	592:601	arg1	degradation					695:705	in vitro degradation	686:705	in vitro degradation	686:705	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	28	theme	biological	592:601	arg1	culture					727:733	cell culture	722:733	cell culture	722:733	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	28	theme	biological	592:601	arg1	spectroscopy					628:639	FTIR spectroscopy	623:639	FTIR spectroscopy	623:639	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	28	theme	biological	592:601	arg1	test					680:683	tensile test	672:683	tensile test	672:683	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	2	29	theme	polypyrrole	358:368	arg1	percentages					343:353	various weight percentages	328:353	various weight percentages of polypyrrole (5, 10, 15, 20, 25%) relative to the polymers combination (chitosan, collagen, and polyethylene oxide)	328:471	In order to prepare these scaffolds, various weight percentages of polypyrrole (5, 10, 15, 20, 25%) relative to the polymers combination (chitosan, collagen, and polyethylene oxide) were used.
33309657	6	30	theme	MTT	1070:1072	arg1	analyses					1082:1089	MTT and SEM analyses	1070:1089	MTT and SEM analyses	1070:1089	MTT and SEM analyses displayed that nanofibers composing 10% polypyrrole possess better cell adhesion, growth and proliferation properties comparing to other compositions.
33309657	6	31	theme	%	1129:1129	arg1	polypyrrole					1131:1141	10% polypyrrole	1127:1141	10% polypyrrole	1127:1141	MTT and SEM analyses displayed that nanofibers composing 10% polypyrrole possess better cell adhesion, growth and proliferation properties comparing to other compositions.
33309657	6	32	theme	proliferation	1184:1196	arg1	properties					1198:1207	better cell adhesion, growth and proliferation properties	1151:1207	better cell adhesion, growth and proliferation properties comparing to other compositions	1151:1239	MTT and SEM analyses displayed that nanofibers composing 10% polypyrrole possess better cell adhesion, growth and proliferation properties comparing to other compositions.
33309657	3	33	theme	electrical	647:656	arg1	analyses					603:610	chemical, morphological, physio-mechanical, and biological analyses	544:610	chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture	544:733	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	33	theme	electrical	647:656	arg1	conductivity					658:669	electrical conductivity	647:669	electrical conductivity	647:669	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	1	34	theme	electrospun	155:165	arg1	polymer					226:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer	144:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer	144:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer was fabricated to accelerate healing of damaged tissues.
33309657	4	35	dep	fibers	818:823	arg1	diameter					833:840	average diameter	825:840	the fibers average diameter	814:840	The polypyrrole particles were perfectly dispersed inside the nanofibers, and the fibers average diameter were reducing by increasing the polypyrrole content in the composites.
33309657	0	36	theme	polypyrrole/chitosan/collagen	47:75	arg1	electrospun					77:87	conductive polypyrrole/chitosan/collagen electrospun	36:87	conductive polypyrrole/chitosan/collagen electrospun	36:87	Fabrication and characterization of conductive polypyrrole/chitosan/collagen electrospun nanofiber scaffold for tissue engineering application.
33309657	7	37	theme	applications	1353:1364	arg1	kinds					1337:1341	different kinds	1327:1341	different kinds of tissue applications including skin, nerve, heart muscle, etc	1327:1405	Furthermore, the suitable mechanical properties of scaffolds ideally fitted them for different kinds of tissue applications including skin, nerve, heart muscle, etc.
33309657	2	38	dep	order	294:298	arg1	prepare					303:309	prepare	303:309	to prepare these scaffolds	300:325	In order to prepare these scaffolds, various weight percentages of polypyrrole (5, 10, 15, 20, 25%) relative to the polymers combination (chitosan, collagen, and polyethylene oxide) were used.
33309657	2	39	theme	polymers	407:414	arg1	combination					416:426	the polymers combination	403:426	the polymers combination (chitosan, collagen, and polyethylene oxide)	403:471	In order to prepare these scaffolds, various weight percentages of polypyrrole (5, 10, 15, 20, 25%) relative to the polymers combination (chitosan, collagen, and polyethylene oxide) were used.
33309657	0	40	theme	conductive	36:45	arg1	electrospun					77:87	conductive polypyrrole/chitosan/collagen electrospun	36:87	conductive polypyrrole/chitosan/collagen electrospun	36:87	Fabrication and characterization of conductive polypyrrole/chitosan/collagen electrospun nanofiber scaffold for tissue engineering application.
33309657	6	41	contain	possess	1143:1149	arg2	properties					1198:1207	better cell adhesion, growth and proliferation properties	1151:1207	better cell adhesion, growth and proliferation properties comparing to other compositions	1151:1239	MTT and SEM analyses displayed that nanofibers composing 10% polypyrrole possess better cell adhesion, growth and proliferation properties comparing to other compositions.
33309657	6	41	contain	possess	1143:1149	arg1	nanofibers					1106:1115	nanofibers	1106:1115	nanofibers composing 10% polypyrrole	1106:1141	MTT and SEM analyses displayed that nanofibers composing 10% polypyrrole possess better cell adhesion, growth and proliferation properties comparing to other compositions.
33309657	1	42	theme	damaged	274:280	arg1	tissues					282:288	damaged tissues	274:288	damaged tissues	274:288	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer was fabricated to accelerate healing of damaged tissues.
33309657	2	43	theme	polyethylene	453:464	arg1	oxide					466:470	polyethylene oxide	453:470	polyethylene oxide	453:470	In order to prepare these scaffolds, various weight percentages of polypyrrole (5, 10, 15, 20, 25%) relative to the polymers combination (chitosan, collagen, and polyethylene oxide) were used.
33309657	3	44	theme	fabricated	488:497	arg1	scaffolds					509:517	The fabricated composite scaffolds	484:517	The fabricated composite scaffolds	484:517	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	5	45	theme	polymers	1060:1067	arg1	range					1020:1024	the range	1016:1024	the range of semi-conductive and conductive polymers	1016:1067	The presence of polypyrrole in fibers enhanced their conductivity up to 164.274 × 10-3 s/m which is in the range of semi-conductive and conductive polymers.
33309657	4	46	theme	average	825:831	arg1	diameter					833:840	average diameter	825:840	the fibers average diameter	814:840	The polypyrrole particles were perfectly dispersed inside the nanofibers, and the fibers average diameter were reducing by increasing the polypyrrole content in the composites.
33309657	6	47	theme	10	1127:1128	arg1	%					1129:1129	%	1129:1129	%	1129:1129	MTT and SEM analyses displayed that nanofibers composing 10% polypyrrole possess better cell adhesion, growth and proliferation properties comparing to other compositions.
33309657	1	48	theme	nanofiber	167:175	arg1	polymer					226:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer	144:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer	144:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer was fabricated to accelerate healing of damaged tissues.
33309657	0	49	theme	electrospun	77:87	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of conductive polypyrrole/chitosan/collagen electrospun nanofiber scaffold for tissue engineering application.
33309657	0	49	theme	electrospun	77:87	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of conductive polypyrrole/chitosan/collagen electrospun nanofiber scaffold for tissue engineering application.
33309657	8	50	theme	conductive	1436:1445	arg1	scaffolds					1457:1465	these fabricated conductive nanofiber scaffolds	1419:1465	these fabricated conductive nanofiber scaffolds	1419:1465	Therefore, these fabricated conductive nanofiber scaffolds are particularly appropriate for employing in body parts with electrical signals such as cardiovascular, heart muscles, or nerves.
33309657	5	51	attach	presence	917:924	arg2	polypyrrole					929:939	polypyrrole	929:939	polypyrrole	929:939	The presence of polypyrrole in fibers enhanced their conductivity up to 164.274 × 10-3 s/m which is in the range of semi-conductive and conductive polymers.
33309657	5	51	attach	presence	917:924	arg1	fibers					944:949	fibers	944:949	fibers	944:949	The presence of polypyrrole in fibers enhanced their conductivity up to 164.274 × 10-3 s/m which is in the range of semi-conductive and conductive polymers.
33309657	5	52	theme	conductive	1049:1058	arg1	polymers					1060:1067	semi-conductive and conductive polymers	1029:1067	semi-conductive and conductive polymers	1029:1067	The presence of polypyrrole in fibers enhanced their conductivity up to 164.274 × 10-3 s/m which is in the range of semi-conductive and conductive polymers.
33309657	2	53	dep	combination	416:426	arg1	collagen					439:446	collagen	439:446	collagen	439:446	In order to prepare these scaffolds, various weight percentages of polypyrrole (5, 10, 15, 20, 25%) relative to the polymers combination (chitosan, collagen, and polyethylene oxide) were used.
33309657	2	53	dep	combination	416:426	arg1	chitosan					429:436	chitosan	429:436	chitosan	429:436	In order to prepare these scaffolds, various weight percentages of polypyrrole (5, 10, 15, 20, 25%) relative to the polymers combination (chitosan, collagen, and polyethylene oxide) were used.
33309657	2	53	dep	combination	416:426	arg1	oxide					466:470	polyethylene oxide	453:470	polyethylene oxide	453:470	In order to prepare these scaffolds, various weight percentages of polypyrrole (5, 10, 15, 20, 25%) relative to the polymers combination (chitosan, collagen, and polyethylene oxide) were used.
33309657	1	54	theme	scaffold	177:184	arg1	polymer					226:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer	144:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer	144:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer was fabricated to accelerate healing of damaged tissues.
33309657	5	55	from	presence	917:924	arg1	fibers					944:949	fibers	944:949	fibers	944:949	The presence of polypyrrole in fibers enhanced their conductivity up to 164.274 × 10-3 s/m which is in the range of semi-conductive and conductive polymers.
33309657	2	56	used	used	478:481	arg2	percentages					343:353	various weight percentages	328:353	various weight percentages of polypyrrole (5, 10, 15, 20, 25%) relative to the polymers combination (chitosan, collagen, and polyethylene oxide)	328:471	In order to prepare these scaffolds, various weight percentages of polypyrrole (5, 10, 15, 20, 25%) relative to the polymers combination (chitosan, collagen, and polyethylene oxide) were used.
33309657	7	57	theme	heart	1389:1393	arg1	muscle					1395:1400	heart muscle	1389:1400	heart muscle	1389:1400	Furthermore, the suitable mechanical properties of scaffolds ideally fitted them for different kinds of tissue applications including skin, nerve, heart muscle, etc.
33309657	7	57	theme	heart	1389:1393	arg1	skin					1376:1379	skin	1376:1379	skin	1376:1379	Furthermore, the suitable mechanical properties of scaffolds ideally fitted them for different kinds of tissue applications including skin, nerve, heart muscle, etc.
33309657	1	58	theme	containing	186:195	arg1	polymer					226:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer	144:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer	144:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer was fabricated to accelerate healing of damaged tissues.
33309657	1	59	theme	Conductive	144:153	arg1	polymer					226:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer	144:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer	144:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer was fabricated to accelerate healing of damaged tissues.
33309657	1	60	theme	tissues	282:288	arg1	healing					263:269	healing	263:269	healing of damaged tissues	263:288	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer was fabricated to accelerate healing of damaged tissues.
33309657	8	61	theme	electrical	1529:1538	arg1	signals					1540:1546	electrical signals	1529:1546	electrical signals such as cardiovascular, heart muscles, or nerves	1529:1595	Therefore, these fabricated conductive nanofiber scaffolds are particularly appropriate for employing in body parts with electrical signals such as cardiovascular, heart muscles, or nerves.
33309657	8	61	theme	electrical	1529:1538	arg1	cardiovascular					1556:1569	cardiovascular	1556:1569	cardiovascular	1556:1569	Therefore, these fabricated conductive nanofiber scaffolds are particularly appropriate for employing in body parts with electrical signals such as cardiovascular, heart muscles, or nerves.
33309657	8	61	theme	electrical	1529:1538	arg1	muscles					1578:1584	heart muscles	1572:1584	heart muscles	1572:1584	Therefore, these fabricated conductive nanofiber scaffolds are particularly appropriate for employing in body parts with electrical signals such as cardiovascular, heart muscles, or nerves.
33309657	8	61	theme	electrical	1529:1538	arg1	nerves					1590:1595	nerves	1590:1595	nerves	1590:1595	Therefore, these fabricated conductive nanofiber scaffolds are particularly appropriate for employing in body parts with electrical signals such as cardiovascular, heart muscles, or nerves.
33309657	8	62	theme	nanofiber	1447:1455	arg1	scaffolds					1457:1465	these fabricated conductive nanofiber scaffolds	1419:1465	these fabricated conductive nanofiber scaffolds	1419:1465	Therefore, these fabricated conductive nanofiber scaffolds are particularly appropriate for employing in body parts with electrical signals such as cardiovascular, heart muscles, or nerves.
33309657	1	63	theme	conductive	197:206	arg1	polymer					226:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer	144:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer	144:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer was fabricated to accelerate healing of damaged tissues.
33309657	4	64	theme	polypyrrole	874:884	arg1	content					886:892	the polypyrrole content	870:892	the polypyrrole content in the composites	870:910	The polypyrrole particles were perfectly dispersed inside the nanofibers, and the fibers average diameter were reducing by increasing the polypyrrole content in the composites.
33309657	8	65	theme	fabricated	1425:1434	arg1	scaffolds					1457:1465	these fabricated conductive nanofiber scaffolds	1419:1465	these fabricated conductive nanofiber scaffolds	1419:1465	Therefore, these fabricated conductive nanofiber scaffolds are particularly appropriate for employing in body parts with electrical signals such as cardiovascular, heart muscles, or nerves.
33309657	3	66	theme	tensile	672:678	arg1	analyses					603:610	chemical, morphological, physio-mechanical, and biological analyses	544:610	chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture	544:733	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	66	theme	tensile	672:678	arg1	test					680:683	tensile test	672:683	tensile test	672:683	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	6	67	theme	other	1222:1226	arg1	compositions					1228:1239	other compositions	1222:1239	other compositions	1222:1239	MTT and SEM analyses displayed that nanofibers composing 10% polypyrrole possess better cell adhesion, growth and proliferation properties comparing to other compositions.
33309657	1	68	theme	polypyrrole	208:218	arg1	polymer					226:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer	144:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer	144:232	Conductive electrospun nanofiber scaffold containing conductive polypyrrole (PPy) polymer was fabricated to accelerate healing of damaged tissues.
33309657	7	69	theme	suitable	1259:1266	arg1	properties					1279:1288	the suitable mechanical properties	1255:1288	the suitable mechanical properties of scaffolds	1255:1301	Furthermore, the suitable mechanical properties of scaffolds ideally fitted them for different kinds of tissue applications including skin, nerve, heart muscle, etc.
33309657	4	70	from	content	886:892	arg1	composites					901:910	the composites	897:910	the composites	897:910	The polypyrrole particles were perfectly dispersed inside the nanofibers, and the fibers average diameter were reducing by increasing the polypyrrole content in the composites.
33309657	3	71	theme	FTIR	623:626	arg1	analyses					603:610	chemical, morphological, physio-mechanical, and biological analyses	544:610	chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture	544:733	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	3	71	theme	FTIR	623:626	arg1	spectroscopy					628:639	FTIR spectroscopy	623:639	FTIR spectroscopy	623:639	The fabricated composite scaffolds were characterized using chemical, morphological, physio-mechanical, and biological analyses including; FTIR spectroscopy, SEM, electrical conductivity, tensile test, in vitro degradation, MTT Assay and cell culture.
33309657	7	72	theme	scaffolds	1293:1301	arg1	properties					1279:1288	the suitable mechanical properties	1255:1288	the suitable mechanical properties of scaffolds	1255:1301	Furthermore, the suitable mechanical properties of scaffolds ideally fitted them for different kinds of tissue applications including skin, nerve, heart muscle, etc.
33309657	7	73	theme	mechanical	1268:1277	arg1	properties					1279:1288	the suitable mechanical properties	1255:1288	the suitable mechanical properties of scaffolds	1255:1301	Furthermore, the suitable mechanical properties of scaffolds ideally fitted them for different kinds of tissue applications including skin, nerve, heart muscle, etc.
33763930	0	0	theme	Stereoselective	86:100	arg1	Synthesis					102:110	Highly Stereoselective Synthesis	79:110	Merging Reagent Modulation and Remote Anchimeric Assistance for Glycosylation: Highly Stereoselective Synthesis of α-Glycans up to a 30-mer.	0:139	Merging Reagent Modulation and Remote Anchimeric Assistance for Glycosylation: Highly Stereoselective Synthesis of α-Glycans up to a 30-mer.
33763930	4	1	theme	complex	956:962	arg1	glycans					964:970	complex glycans	956:970	complex glycans	956:970	Orthogonal one-pot glycosylation strategy based on glycosyl ortho-alkynylbenzoates and ortho-(1-phenylvinyl)benzoates has been demonstrated in the efficient synthesis of complex glycans, precluding such issues as aglycon transfer inherent to orthogonal one-pot synthesis based on thioglycosides.
33763930	2	2	theme	assistance	370:379	arg1	strategy					395:402	a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy	304:402	strategy	395:402	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	2	3	theme	glycans	520:526	arg1	synthesis					471:479	the first highly stereoselective synthesis	438:479	the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds	438:612	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	2	4	theme	anchimeric	359:368	arg1	strategy					395:402	a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy	304:402	strategy	395:402	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	2	5	dep	30	575:576	arg1	to					572:573	to	572:573	to	572:573	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	4	6	theme	ortho-	873:878	arg1	benzoates					894:902	ortho-(1-phenylvinyl)benzoates	873:902	ortho-(1-phenylvinyl)benzoates	873:902	Orthogonal one-pot glycosylation strategy based on glycosyl ortho-alkynylbenzoates and ortho-(1-phenylvinyl)benzoates has been demonstrated in the efficient synthesis of complex glycans, precluding such issues as aglycon transfer inherent to orthogonal one-pot synthesis based on thioglycosides.
33763930	4	7	theme	glycosyl	837:844	arg1	ortho-alkynylbenzoates					846:867	glycosyl ortho-alkynylbenzoates	837:867	glycosyl ortho-alkynylbenzoates	837:867	Orthogonal one-pot glycosylation strategy based on glycosyl ortho-alkynylbenzoates and ortho-(1-phenylvinyl)benzoates has been demonstrated in the efficient synthesis of complex glycans, precluding such issues as aglycon transfer inherent to orthogonal one-pot synthesis based on thioglycosides.
33763930	1	8	theme	long-standing	263:275	arg1	synthesis					155:163	The efficient synthesis	141:163	The efficient synthesis of long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages	141:256	The efficient synthesis of long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages is a long-standing challenge.
33763930	1	8	theme	long-standing	263:275	arg1	challenge					277:285	a long-standing challenge	261:285	a long-standing challenge	261:285	The efficient synthesis of long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages is a long-standing challenge.
33763930	4	9	theme	glycosylation	805:817	arg1	strategy					819:826	Orthogonal one-pot glycosylation strategy	786:826	Orthogonal one-pot glycosylation strategy based on glycosyl ortho-alkynylbenzoates and ortho-(1-phenylvinyl)benzoates	786:902	Orthogonal one-pot glycosylation strategy based on glycosyl ortho-alkynylbenzoates and ortho-(1-phenylvinyl)benzoates has been demonstrated in the efficient synthesis of complex glycans, precluding such issues as aglycon transfer inherent to orthogonal one-pot synthesis based on thioglycosides.
33763930	4	10	theme	glycans	964:970	arg1	synthesis					943:951	the efficient synthesis	929:951	the efficient synthesis of complex glycans	929:970	Orthogonal one-pot glycosylation strategy based on glycosyl ortho-alkynylbenzoates and ortho-(1-phenylvinyl)benzoates has been demonstrated in the efficient synthesis of complex glycans, precluding such issues as aglycon transfer inherent to orthogonal one-pot synthesis based on thioglycosides.
33763930	2	11	theme	glycans	550:556	arg1	synthesis					471:479	the first highly stereoselective synthesis	438:479	the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds	438:612	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	3	12	with	glucosylation	707:719	arg1	group					741:745	6-O-levulinoyl group	726:745	6-O-levulinoyl group than 6-O-acetyl or 6-O-benzoyl groups	726:783	DFT calculations shed light on the origin of the much higher stereoselectivities of 1,2-cis glucosylation with 6-O-levulinoyl group than 6-O-acetyl or 6-O-benzoyl groups.
33763930	1	13	theme	long	168:171	arg1	carbohydrates					196:208	long, branched, and complex carbohydrates	168:208	long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages	168:256	The efficient synthesis of long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages is a long-standing challenge.
33763930	0	14	theme	α-Glycans	115:123	arg1	Synthesis					102:110	Highly Stereoselective Synthesis	79:110	Merging Reagent Modulation and Remote Anchimeric Assistance for Glycosylation: Highly Stereoselective Synthesis of α-Glycans up to a 30-mer.	0:139	Merging Reagent Modulation and Remote Anchimeric Assistance for Glycosylation: Highly Stereoselective Synthesis of α-Glycans up to a 30-mer.
33763930	3	15	theme	6-O-benzoyl	766:776	arg1	groups					778:783	6-O-acetyl or 6-O-benzoyl groups	752:783	6-O-acetyl or 6-O-benzoyl groups	752:783	DFT calculations shed light on the origin of the much higher stereoselectivities of 1,2-cis glucosylation with 6-O-levulinoyl group than 6-O-acetyl or 6-O-benzoyl groups.
33763930	2	16	theme	Huoshanense	508:518	arg1	glycans					520:526	the branched Dendrobium Huoshanense glycans	484:526	the branched Dendrobium Huoshanense glycans	484:526	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	0	17	theme	Reagent	8:14	arg1	Modulation					16:25	Merging Reagent Modulation	0:25	Merging Reagent Modulation	0:25	Merging Reagent Modulation and Remote Anchimeric Assistance for Glycosylation: Highly Stereoselective Synthesis of α-Glycans up to a 30-mer.
33763930	2	18	theme	reagent	314:320	arg1	modulation					322:331	a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy	304:402	modulation	322:331	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	1	19	theme	branched	174:181	arg1	carbohydrates					196:208	long, branched, and complex carbohydrates	168:208	long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages	168:256	The efficient synthesis of long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages is a long-standing challenge.
33763930	0	20	theme	Merging	0:6	arg1	Modulation					16:25	Merging Reagent Modulation	0:25	Merging Reagent Modulation	0:25	Merging Reagent Modulation and Remote Anchimeric Assistance for Glycosylation: Highly Stereoselective Synthesis of α-Glycans up to a 30-mer.
33763930	4	21	theme	efficient	933:941	arg1	synthesis					943:951	the efficient synthesis	929:951	the efficient synthesis of complex glycans	929:970	Orthogonal one-pot glycosylation strategy based on glycosyl ortho-alkynylbenzoates and ortho-(1-phenylvinyl)benzoates has been demonstrated in the efficient synthesis of complex glycans, precluding such issues as aglycon transfer inherent to orthogonal one-pot synthesis based on thioglycosides.
33763930	2	22	theme	merging	306:312	arg1	modulation					322:331	a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy	304:402	modulation	322:331	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	2	23	theme	stereoselective	455:469	arg1	synthesis					471:479	the first highly stereoselective synthesis	438:479	the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds	438:612	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	4	24	theme	one-pot	797:803	arg1	strategy					819:826	Orthogonal one-pot glycosylation strategy	786:826	Orthogonal one-pot glycosylation strategy based on glycosyl ortho-alkynylbenzoates and ortho-(1-phenylvinyl)benzoates	786:902	Orthogonal one-pot glycosylation strategy based on glycosyl ortho-alkynylbenzoates and ortho-(1-phenylvinyl)benzoates has been demonstrated in the efficient synthesis of complex glycans, precluding such issues as aglycon transfer inherent to orthogonal one-pot synthesis based on thioglycosides.
33763930	2	25	theme	1,2-cis	589:595	arg1	bonds					608:612	up to 30 contiguous 1,2-cis glucosidic bonds	569:612	up to 30 contiguous 1,2-cis glucosidic bonds	569:612	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	2	26	theme	first	442:446	arg1	synthesis					471:479	the first highly stereoselective synthesis	438:479	the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds	438:612	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	3	27	theme	DFT	615:617	arg1	calculations					619:630	DFT calculations	615:630	DFT calculations	615:630	DFT calculations shed light on the origin of the much higher stereoselectivities of 1,2-cis glucosylation with 6-O-levulinoyl group than 6-O-acetyl or 6-O-benzoyl groups.
33763930	2	28	theme	contiguous	578:587	arg1	bonds					608:612	up to 30 contiguous 1,2-cis glucosidic bonds	569:612	up to 30 contiguous 1,2-cis glucosidic bonds	569:612	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	3	29	theme	stereoselectivities	676:694	arg1	origin					650:655	the origin	646:655	the origin of the much higher stereoselectivities of 1,2-cis glucosylation with 6-O-levulinoyl group than 6-O-acetyl or 6-O-benzoyl groups	646:783	DFT calculations shed light on the origin of the much higher stereoselectivities of 1,2-cis glucosylation with 6-O-levulinoyl group than 6-O-acetyl or 6-O-benzoyl groups.
33763930	1	30	theme	complex	188:194	arg1	carbohydrates					196:208	long, branched, and complex carbohydrates	168:208	long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages	168:256	The efficient synthesis of long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages is a long-standing challenge.
33763930	0	31	theme	Anchimeric	38:47	arg1	Assistance					49:58	Remote Anchimeric Assistance	31:58	Remote Anchimeric Assistance	31:58	Merging Reagent Modulation and Remote Anchimeric Assistance for Glycosylation: Highly Stereoselective Synthesis of α-Glycans up to a 30-mer.
33763930	1	32	theme	carbohydrates	196:208	arg1	synthesis					155:163	The efficient synthesis	141:163	The efficient synthesis of long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages	141:256	The efficient synthesis of long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages is a long-standing challenge.
33763930	1	32	theme	carbohydrates	196:208	arg1	challenge					277:285	a long-standing challenge	261:285	a long-standing challenge	261:285	The efficient synthesis of long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages is a long-standing challenge.
33763930	0	33	theme	Remote	31:36	arg1	Assistance					49:58	Remote Anchimeric Assistance	31:58	Remote Anchimeric Assistance	31:58	Merging Reagent Modulation and Remote Anchimeric Assistance for Glycosylation: Highly Stereoselective Synthesis of α-Glycans up to a 30-mer.
33763930	2	34	theme	Dendrobium	497:506	arg1	glycans					520:526	the branched Dendrobium Huoshanense glycans	484:526	the branched Dendrobium Huoshanense glycans	484:526	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	2	35	theme	Longan	543:548	arg1	glycans					550:556	the linear Longan glycans	532:556	the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds	532:612	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	2	36	theme	branched	488:495	arg1	glycans					520:526	the branched Dendrobium Huoshanense glycans	484:526	the branched Dendrobium Huoshanense glycans	484:526	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	4	37	theme	one-pot	1039:1045	arg1	synthesis					1047:1055	orthogonal one-pot synthesis	1028:1055	orthogonal one-pot synthesis based on thioglycosides	1028:1079	Orthogonal one-pot glycosylation strategy based on glycosyl ortho-alkynylbenzoates and ortho-(1-phenylvinyl)benzoates has been demonstrated in the efficient synthesis of complex glycans, precluding such issues as aglycon transfer inherent to orthogonal one-pot synthesis based on thioglycosides.
33763930	4	38	theme	such	984:987	arg1	issues					989:994	such issues	984:994	such issues	984:994	Orthogonal one-pot glycosylation strategy based on glycosyl ortho-alkynylbenzoates and ortho-(1-phenylvinyl)benzoates has been demonstrated in the efficient synthesis of complex glycans, precluding such issues as aglycon transfer inherent to orthogonal one-pot synthesis based on thioglycosides.
33763930	3	39	theme	higher	669:674	arg1	stereoselectivities					676:694	the much higher stereoselectivities	660:694	the much higher stereoselectivities of 1,2-cis glucosylation with 6-O-levulinoyl group than 6-O-acetyl or 6-O-benzoyl groups	660:783	DFT calculations shed light on the origin of the much higher stereoselectivities of 1,2-cis glucosylation with 6-O-levulinoyl group than 6-O-acetyl or 6-O-benzoyl groups.
33763930	2	40	theme	remote	352:357	arg1	strategy					395:402	a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy	304:402	strategy	395:402	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	4	41	theme	inherent	1016:1023	arg1	transfer					1007:1014	aglycon transfer	999:1014	aglycon transfer inherent to orthogonal one-pot synthesis based on thioglycosides	999:1079	Orthogonal one-pot glycosylation strategy based on glycosyl ortho-alkynylbenzoates and ortho-(1-phenylvinyl)benzoates has been demonstrated in the efficient synthesis of complex glycans, precluding such issues as aglycon transfer inherent to orthogonal one-pot synthesis based on thioglycosides.
33763930	1	42	contain	containing	210:219	arg2	linkages					249:256	multiple 1,2-cis glycosidic linkages	221:256	multiple 1,2-cis glycosidic linkages	221:256	The efficient synthesis of long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages is a long-standing challenge.
33763930	1	42	contain	containing	210:219	arg1	carbohydrates					196:208	long, branched, and complex carbohydrates	168:208	long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages	168:256	The efficient synthesis of long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages is a long-standing challenge.
33763930	1	43	theme	efficient	145:153	arg1	synthesis					155:163	The efficient synthesis	141:163	The efficient synthesis of long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages	141:256	The efficient synthesis of long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages is a long-standing challenge.
33763930	1	43	theme	efficient	145:153	arg1	challenge					277:285	a long-standing challenge	261:285	a long-standing challenge	261:285	The efficient synthesis of long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages is a long-standing challenge.
33763930	2	44	theme	6-O-levulinoyl	337:350	arg1	strategy					395:402	a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy	304:402	strategy	395:402	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	3	45	theme	6-O-levulinoyl	726:739	arg1	group					741:745	6-O-levulinoyl group	726:745	6-O-levulinoyl group than 6-O-acetyl or 6-O-benzoyl groups	726:783	DFT calculations shed light on the origin of the much higher stereoselectivities of 1,2-cis glucosylation with 6-O-levulinoyl group than 6-O-acetyl or 6-O-benzoyl groups.
33763930	4	46	theme	Orthogonal	786:795	arg1	strategy					819:826	Orthogonal one-pot glycosylation strategy	786:826	Orthogonal one-pot glycosylation strategy based on glycosyl ortho-alkynylbenzoates and ortho-(1-phenylvinyl)benzoates	786:902	Orthogonal one-pot glycosylation strategy based on glycosyl ortho-alkynylbenzoates and ortho-(1-phenylvinyl)benzoates has been demonstrated in the efficient synthesis of complex glycans, precluding such issues as aglycon transfer inherent to orthogonal one-pot synthesis based on thioglycosides.
33763930	1	47	theme	multiple	221:228	arg1	linkages					249:256	multiple 1,2-cis glycosidic linkages	221:256	multiple 1,2-cis glycosidic linkages	221:256	The efficient synthesis of long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages is a long-standing challenge.
33763930	2	48	theme	glucosidic	597:606	arg1	bonds					608:612	up to 30 contiguous 1,2-cis glucosidic bonds	569:612	up to 30 contiguous 1,2-cis glucosidic bonds	569:612	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	3	49	theme	1,2-cis	699:705	arg1	glucosylation					707:719	1,2-cis glucosylation	699:719	1,2-cis glucosylation with 6-O-levulinoyl group than 6-O-acetyl or 6-O-benzoyl groups	699:783	DFT calculations shed light on the origin of the much higher stereoselectivities of 1,2-cis glucosylation with 6-O-levulinoyl group than 6-O-acetyl or 6-O-benzoyl groups.
33763930	0	50	dep	Modulation	16:25	arg1	Synthesis					102:110	Highly Stereoselective Synthesis	79:110	Merging Reagent Modulation and Remote Anchimeric Assistance for Glycosylation: Highly Stereoselective Synthesis of α-Glycans up to a 30-mer.	0:139	Merging Reagent Modulation and Remote Anchimeric Assistance for Glycosylation: Highly Stereoselective Synthesis of α-Glycans up to a 30-mer.
33763930	0	51	dep	30-mer	133:138	arg1	up					125:126	up	125:126	up	125:126	Merging Reagent Modulation and Remote Anchimeric Assistance for Glycosylation: Highly Stereoselective Synthesis of α-Glycans up to a 30-mer.
33763930	4	52	theme	aglycon	999:1005	arg1	transfer					1007:1014	aglycon transfer	999:1014	aglycon transfer inherent to orthogonal one-pot synthesis based on thioglycosides	999:1079	Orthogonal one-pot glycosylation strategy based on glycosyl ortho-alkynylbenzoates and ortho-(1-phenylvinyl)benzoates has been demonstrated in the efficient synthesis of complex glycans, precluding such issues as aglycon transfer inherent to orthogonal one-pot synthesis based on thioglycosides.
33763930	1	53	theme	1,2-cis	230:236	arg1	linkages					249:256	multiple 1,2-cis glycosidic linkages	221:256	multiple 1,2-cis glycosidic linkages	221:256	The efficient synthesis of long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages is a long-standing challenge.
33763930	2	54	theme	linear	536:541	arg1	glycans					550:556	the linear Longan glycans	532:556	the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds	532:612	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	3	55	theme	glucosylation	707:719	arg1	stereoselectivities					676:694	the much higher stereoselectivities	660:694	the much higher stereoselectivities of 1,2-cis glucosylation with 6-O-levulinoyl group than 6-O-acetyl or 6-O-benzoyl groups	660:783	DFT calculations shed light on the origin of the much higher stereoselectivities of 1,2-cis glucosylation with 6-O-levulinoyl group than 6-O-acetyl or 6-O-benzoyl groups.
33763930	1	56	theme	glycosidic	238:247	arg1	linkages					249:256	multiple 1,2-cis glycosidic linkages	221:256	multiple 1,2-cis glycosidic linkages	221:256	The efficient synthesis of long, branched, and complex carbohydrates containing multiple 1,2-cis glycosidic linkages is a long-standing challenge.
33763930	3	57	theme	6-O-acetyl	752:761	arg1	groups					778:783	6-O-acetyl or 6-O-benzoyl groups	752:783	6-O-acetyl or 6-O-benzoyl groups	752:783	DFT calculations shed light on the origin of the much higher stereoselectivities of 1,2-cis glucosylation with 6-O-levulinoyl group than 6-O-acetyl or 6-O-benzoyl groups.
33763930	4	58	theme	1-phenylvinyl	880:892	arg1	benzoates					894:902	ortho-(1-phenylvinyl)benzoates	873:902	ortho-(1-phenylvinyl)benzoates	873:902	Orthogonal one-pot glycosylation strategy based on glycosyl ortho-alkynylbenzoates and ortho-(1-phenylvinyl)benzoates has been demonstrated in the efficient synthesis of complex glycans, precluding such issues as aglycon transfer inherent to orthogonal one-pot synthesis based on thioglycosides.
33763930	2	59	contain	containing	558:567	arg1	glycans					550:556	the linear Longan glycans	532:556	the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds	532:612	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	2	59	contain	containing	558:567	arg2	bonds					608:612	up to 30 contiguous 1,2-cis glucosidic bonds	569:612	up to 30 contiguous 1,2-cis glucosidic bonds	569:612	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	2	60	theme	glycosylation	381:393	arg1	strategy					395:402	a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy	304:402	strategy	395:402	Here, we report a merging reagent modulation and 6-O-levulinoyl remote anchimeric assistance glycosylation strategy, which is successfully applied to the first highly stereoselective synthesis of the branched Dendrobium Huoshanense glycans and the linear Longan glycans containing up to 30 contiguous 1,2-cis glucosidic bonds.
33763930	4	61	theme	orthogonal	1028:1037	arg1	synthesis					1047:1055	orthogonal one-pot synthesis	1028:1055	orthogonal one-pot synthesis based on thioglycosides	1028:1079	Orthogonal one-pot glycosylation strategy based on glycosyl ortho-alkynylbenzoates and ortho-(1-phenylvinyl)benzoates has been demonstrated in the efficient synthesis of complex glycans, precluding such issues as aglycon transfer inherent to orthogonal one-pot synthesis based on thioglycosides.
32979138	10	0	theme	expression	1196:1205	arg1	level					1207:1211	the expression level	1192:1211	the expression level of welK	1192:1219	The polysaccharide composition and qRT-PCR analysis showed that the glucuronic acid content was closely related to the expression level of welK.
32979138	12	1	theme	manipulation	1386:1397	arg1	method					1399:1404	a genetic manipulation method	1376:1404	a genetic manipulation method for the functional characterization of genes in Sphingomonas sp	1376:1468	Our experiment provided a genetic manipulation method for the functional characterization of genes in Sphingomonas sp.
32979138	11	2	theme	gum	1278:1280	arg1	synthesis					1282:1290	the WL gum synthesis	1271:1290	the WL gum synthesis	1271:1290	Thus, WelK was proved to play a critical role in the WL gum synthesis and will be an attractive target for metabolic engineering.
32979138	8	3	theme	Homologous	795:804	arg1	expression					806:815	Homologous expression	795:815	Homologous expression of welK in the native organism	795:846	Homologous expression of welK in the native organism can effectively improve WL gum production.
32979138	0	4	theme	Biosynthesis	74:85	arg1	Process					87:93	the Sphingan WL Gum Biosynthesis Process	54:93	the Sphingan WL Gum Biosynthesis Process in Marine Sphingomonas sp	54:119	The Function of β-1,4-Glucuronosyltransferase WelK in the Sphingan WL Gum Biosynthesis Process in Marine Sphingomonas sp.
32979138	0	5	from	Function	4:11	arg1	Process					87:93	the Sphingan WL Gum Biosynthesis Process	54:93	the Sphingan WL Gum Biosynthesis Process in Marine Sphingomonas sp	54:119	The Function of β-1,4-Glucuronosyltransferase WelK in the Sphingan WL Gum Biosynthesis Process in Marine Sphingomonas sp.
32979138	6	6	theme	wild	599:602	arg1	strain					604:609	the wild strain	595:609	the wild strain	595:609	Compared to the wild strain, the WL gum production and broth viscosity of the mutant ∆welK were decreased by 71.5% and 99.2% when cultured for 48 h.
32979138	3	7	from	industries	257:266	arg1	potential					225:233	potential	225:233	potential	225:233	WG showed commercial utility potential in ink, food, and oil industries.
32979138	0	8	theme	Marine	98:103	arg1	sp					118:119	Marine Sphingomonas sp	98:119	Marine Sphingomonas sp	98:119	The Function of β-1,4-Glucuronosyltransferase WelK in the Sphingan WL Gum Biosynthesis Process in Marine Sphingomonas sp.
32979138	4	9	theme	UDP-glucuronic	369:382	arg1	acid					384:387	UDP-glucuronic acid	369:387	UDP-glucuronic acid	369:387	A β-1,4-glucuronosyltransferase WelK was predicted to catalyze the transfer of glucuronic acid from UDP-glucuronic acid to glucosyl-α-pyrophosphorylpolyprenol intermediate in the WL gum biosynthesis process.
32979138	10	10	theme	welK	1216:1219	arg1	level					1207:1211	the expression level	1192:1211	the expression level of welK	1192:1219	The polysaccharide composition and qRT-PCR analysis showed that the glucuronic acid content was closely related to the expression level of welK.
32979138	9	11	theme	gum	935:937	arg1	production					939:948	the WL gum production	928:948	the WL gum production by the welK-overexpressing strain cultured for 60 h and 84 h	928:1009	When glucose concentration was 6.7%, the WL gum production by the welK-overexpressing strain cultured for 60 h and 84 h reached 32.65 and 43.13 g/L, 134.1%, and 114% of the wild strain.
32979138	3	12	theme	potential	225:233	arg1	utility					217:223	commercial utility	206:223	commercial utility potential in ink, food, and oil industries	206:266	WG showed commercial utility potential in ink, food, and oil industries.
32979138	4	13	theme	WL	448:449	arg1	process					468:474	the WL gum biosynthesis process	444:474	the WL gum biosynthesis process	444:474	A β-1,4-glucuronosyltransferase WelK was predicted to catalyze the transfer of glucuronic acid from UDP-glucuronic acid to glucosyl-α-pyrophosphorylpolyprenol intermediate in the WL gum biosynthesis process.
32979138	5	14	theme	gene	533:536	arg1	knocking					538:545	gene knocking	533:545	gene knocking out	533:549	Its function was evaluated by bioinformatical analysis, gene knocking out, and overexpressing strategies.
32979138	11	15	theme	metabolic	1329:1337	arg1	engineering					1339:1349	metabolic engineering	1329:1349	metabolic engineering	1329:1349	Thus, WelK was proved to play a critical role in the WL gum synthesis and will be an attractive target for metabolic engineering.
32979138	2	16	theme	WL	160:161	arg1	gum					163:165	The marine-derived polysaccharide WL gum	126:165	The marine-derived polysaccharide WL gum	126:165	The marine-derived polysaccharide WL gum produced by Sphingomonas sp.
32979138	11	17	theme	critical	1254:1261	arg1	role					1263:1266	a critical role	1252:1266	a critical role	1252:1266	Thus, WelK was proved to play a critical role in the WL gum synthesis and will be an attractive target for metabolic engineering.
32979138	4	18	theme	β-1,4-glucuronosyltransferase	271:299	arg1	WelK					301:304	A β-1,4-glucuronosyltransferase WelK	269:304	A β-1,4-glucuronosyltransferase WelK	269:304	A β-1,4-glucuronosyltransferase WelK was predicted to catalyze the transfer of glucuronic acid from UDP-glucuronic acid to glucosyl-α-pyrophosphorylpolyprenol intermediate in the WL gum biosynthesis process.
32979138	0	19	theme	Sphingomonas	105:116	arg1	sp					118:119	Marine Sphingomonas sp	98:119	Marine Sphingomonas sp	98:119	The Function of β-1,4-Glucuronosyltransferase WelK in the Sphingan WL Gum Biosynthesis Process in Marine Sphingomonas sp.
32979138	2	20	theme	polysaccharide	145:158	arg1	gum					163:165	The marine-derived polysaccharide WL gum	126:165	The marine-derived polysaccharide WL gum	126:165	The marine-derived polysaccharide WL gum produced by Sphingomonas sp.
32979138	12	21	from	genes	1445:1449	arg1	sp					1467:1468	Sphingomonas sp	1454:1468	Sphingomonas sp	1454:1468	Our experiment provided a genetic manipulation method for the functional characterization of genes in Sphingomonas sp.
32979138	9	22	theme	welK-overexpressing	957:975	arg1	strain					977:982	the welK-overexpressing strain	953:982	the welK-overexpressing strain cultured for 60 h and 84 h	953:1009	When glucose concentration was 6.7%, the WL gum production by the welK-overexpressing strain cultured for 60 h and 84 h reached 32.65 and 43.13 g/L, 134.1%, and 114% of the wild strain.
32979138	6	23	theme	broth	638:642	arg1	viscosity					644:652	broth viscosity	638:652	broth viscosity	638:652	Compared to the wild strain, the WL gum production and broth viscosity of the mutant ∆welK were decreased by 71.5% and 99.2% when cultured for 48 h.
32979138	2	24	theme	marine-derived	130:143	arg1	gum					163:165	The marine-derived polysaccharide WL gum	126:165	The marine-derived polysaccharide WL gum	126:165	The marine-derived polysaccharide WL gum produced by Sphingomonas sp.
32979138	3	25	theme	commercial	206:215	arg1	utility					217:223	commercial utility	206:223	commercial utility potential in ink, food, and oil industries	206:266	WG showed commercial utility potential in ink, food, and oil industries.
32979138	9	26	theme	glucose	896:902	arg1	concentration					904:916	glucose concentration	896:916	glucose concentration	896:916	When glucose concentration was 6.7%, the WL gum production by the welK-overexpressing strain cultured for 60 h and 84 h reached 32.65 and 43.13 g/L, 134.1%, and 114% of the wild strain.
32979138	9	27	theme	WL	932:933	arg1	production					939:948	the WL gum production	928:948	the WL gum production by the welK-overexpressing strain cultured for 60 h and 84 h	928:1009	When glucose concentration was 6.7%, the WL gum production by the welK-overexpressing strain cultured for 60 h and 84 h reached 32.65 and 43.13 g/L, 134.1%, and 114% of the wild strain.
32979138	4	28	theme	glucosyl-α-pyrophosphorylpolyprenol	392:426	arg1	intermediate					428:439	glucosyl-α-pyrophosphorylpolyprenol intermediate	392:439	glucosyl-α-pyrophosphorylpolyprenol intermediate	392:439	A β-1,4-glucuronosyltransferase WelK was predicted to catalyze the transfer of glucuronic acid from UDP-glucuronic acid to glucosyl-α-pyrophosphorylpolyprenol intermediate in the WL gum biosynthesis process.
32979138	0	29	theme	β-1,4-Glucuronosyltransferase	16:44	arg1	WelK					46:49	β-1,4-Glucuronosyltransferase WelK	16:49	β-1,4-Glucuronosyltransferase WelK	16:49	The Function of β-1,4-Glucuronosyltransferase WelK in the Sphingan WL Gum Biosynthesis Process in Marine Sphingomonas sp.
32979138	3	30	from	food	243:246	arg1	potential					225:233	potential	225:233	potential	225:233	WG showed commercial utility potential in ink, food, and oil industries.
32979138	4	31	from	acid	384:387	arg1	transfer					336:343	the transfer	332:343	the transfer of glucuronic acid from UDP-glucuronic acid to glucosyl-α-pyrophosphorylpolyprenol intermediate	332:439	A β-1,4-glucuronosyltransferase WelK was predicted to catalyze the transfer of glucuronic acid from UDP-glucuronic acid to glucosyl-α-pyrophosphorylpolyprenol intermediate in the WL gum biosynthesis process.
32979138	5	32	theme	bioinformatical	507:521	arg1	analysis					523:530	bioinformatical analysis	507:530	bioinformatical analysis	507:530	Its function was evaluated by bioinformatical analysis, gene knocking out, and overexpressing strategies.
32979138	6	33	theme	gum	619:621	arg1	production					623:632	WL gum production	616:632	WL gum production	616:632	Compared to the wild strain, the WL gum production and broth viscosity of the mutant ∆welK were decreased by 71.5% and 99.2% when cultured for 48 h.
32979138	10	34	theme	polysaccharide	1081:1094	arg1	composition					1096:1106	The polysaccharide composition and qRT-PCR analysis	1077:1127	composition	1096:1106	The polysaccharide composition and qRT-PCR analysis showed that the glucuronic acid content was closely related to the expression level of welK.
32979138	0	35	from	Process	87:93	arg1	sp					118:119	Marine Sphingomonas sp	98:119	Marine Sphingomonas sp	98:119	The Function of β-1,4-Glucuronosyltransferase WelK in the Sphingan WL Gum Biosynthesis Process in Marine Sphingomonas sp.
32979138	9	36	theme	strain	1069:1074	arg1	%					1055:1055	114%	1052:1055	114% of the wild strain	1052:1074	When glucose concentration was 6.7%, the WL gum production by the welK-overexpressing strain cultured for 60 h and 84 h reached 32.65 and 43.13 g/L, 134.1%, and 114% of the wild strain.
32979138	9	36	theme	strain	1069:1074	arg1	strain					1069:1074	the wild strain	1060:1074	the wild strain	1060:1074	When glucose concentration was 6.7%, the WL gum production by the welK-overexpressing strain cultured for 60 h and 84 h reached 32.65 and 43.13 g/L, 134.1%, and 114% of the wild strain.
32979138	9	36	theme	strain	1069:1074	arg1	43.13 g/L					1029:1037	43.13 g/L	1029:1037	43.13 g/L	1029:1037	When glucose concentration was 6.7%, the WL gum production by the welK-overexpressing strain cultured for 60 h and 84 h reached 32.65 and 43.13 g/L, 134.1%, and 114% of the wild strain.
32979138	9	36	theme	strain	1069:1074	arg1	%					1045:1045	134.1%	1040:1045	134.1%	1040:1045	When glucose concentration was 6.7%, the WL gum production by the welK-overexpressing strain cultured for 60 h and 84 h reached 32.65 and 43.13 g/L, 134.1%, and 114% of the wild strain.
32979138	9	36	theme	strain	1069:1074	arg1	32.65					1019:1023	32.65	1019:1023	32.65	1019:1023	When glucose concentration was 6.7%, the WL gum production by the welK-overexpressing strain cultured for 60 h and 84 h reached 32.65 and 43.13 g/L, 134.1%, and 114% of the wild strain.
32979138	6	37	theme	WL	616:617	arg1	production					623:632	WL gum production	616:632	WL gum production	616:632	Compared to the wild strain, the WL gum production and broth viscosity of the mutant ∆welK were decreased by 71.5% and 99.2% when cultured for 48 h.
32979138	8	38	theme	WL	872:873	arg1	production					879:888	WL gum production	872:888	WL gum production	872:888	Homologous expression of welK in the native organism can effectively improve WL gum production.
32979138	3	39	theme	oil	253:255	arg1	industries					257:266	oil industries	253:266	oil industries	253:266	WG showed commercial utility potential in ink, food, and oil industries.
32979138	2	40	theme	Sphingomonas	179:190	arg1	sp					192:193	Sphingomonas sp	179:193	Sphingomonas sp	179:193	The marine-derived polysaccharide WL gum produced by Sphingomonas sp.
32979138	0	41	theme	WelK	46:49	arg1	Function					4:11	The Function	0:11	The Function of β-1,4-Glucuronosyltransferase WelK in the Sphingan WL Gum Biosynthesis Process in Marine Sphingomonas sp.	0:120	The Function of β-1,4-Glucuronosyltransferase WelK in the Sphingan WL Gum Biosynthesis Process in Marine Sphingomonas sp.
32979138	7	42	theme	product	774:780	arg1	preparation					782:792	product preparation	774:792	product preparation	774:792	The gene disruption led to the failure of product preparation.
32979138	7	43	theme	gene	736:739	arg1	disruption					741:750	The gene disruption	732:750	The gene disruption	732:750	The gene disruption led to the failure of product preparation.
32979138	4	44	theme	glucuronic	348:357	arg1	acid					359:362	glucuronic acid	348:362	glucuronic acid	348:362	A β-1,4-glucuronosyltransferase WelK was predicted to catalyze the transfer of glucuronic acid from UDP-glucuronic acid to glucosyl-α-pyrophosphorylpolyprenol intermediate in the WL gum biosynthesis process.
32979138	10	45	theme	qRT-PCR	1112:1118	arg1	analysis					1120:1127	The polysaccharide composition and qRT-PCR analysis	1077:1127	analysis	1120:1127	The polysaccharide composition and qRT-PCR analysis showed that the glucuronic acid content was closely related to the expression level of welK.
32979138	0	46	theme	Sphingan	58:65	arg1	Process					87:93	the Sphingan WL Gum Biosynthesis Process	54:93	the Sphingan WL Gum Biosynthesis Process in Marine Sphingomonas sp	54:119	The Function of β-1,4-Glucuronosyltransferase WelK in the Sphingan WL Gum Biosynthesis Process in Marine Sphingomonas sp.
32979138	3	47	from	potential	225:233	arg1	food					243:246	food	243:246	food	243:246	WG showed commercial utility potential in ink, food, and oil industries.
32979138	3	47	from	potential	225:233	arg1	ink					238:240	ink	238:240	ink	238:240	WG showed commercial utility potential in ink, food, and oil industries.
32979138	3	47	from	potential	225:233	arg1	industries					257:266	oil industries	253:266	oil industries	253:266	WG showed commercial utility potential in ink, food, and oil industries.
32979138	6	48	dep	production	623:632	arg1	the					612:614	the	612:614	the	612:614	Compared to the wild strain, the WL gum production and broth viscosity of the mutant ∆welK were decreased by 71.5% and 99.2% when cultured for 48 h.
32979138	12	49	theme	genetic	1378:1384	arg1	method					1399:1404	a genetic manipulation method	1376:1404	a genetic manipulation method for the functional characterization of genes in Sphingomonas sp	1376:1468	Our experiment provided a genetic manipulation method for the functional characterization of genes in Sphingomonas sp.
32979138	8	50	from	expression	806:815	arg1	organism					839:846	the native organism	828:846	the native organism	828:846	Homologous expression of welK in the native organism can effectively improve WL gum production.
32979138	4	51	theme	biosynthesis	455:466	arg1	process					468:474	the WL gum biosynthesis process	444:474	the WL gum biosynthesis process	444:474	A β-1,4-glucuronosyltransferase WelK was predicted to catalyze the transfer of glucuronic acid from UDP-glucuronic acid to glucosyl-α-pyrophosphorylpolyprenol intermediate in the WL gum biosynthesis process.
32979138	12	52	theme	functional	1414:1423	arg1	characterization					1425:1440	the functional characterization	1410:1440	the functional characterization of genes in Sphingomonas sp	1410:1468	Our experiment provided a genetic manipulation method for the functional characterization of genes in Sphingomonas sp.
32979138	12	53	theme	Sphingomonas	1454:1465	arg1	sp					1467:1468	Sphingomonas sp	1454:1468	Sphingomonas sp	1454:1468	Our experiment provided a genetic manipulation method for the functional characterization of genes in Sphingomonas sp.
32979138	3	54	from	ink	238:240	arg1	potential					225:233	potential	225:233	potential	225:233	WG showed commercial utility potential in ink, food, and oil industries.
32979138	4	55	theme	gum	451:453	arg1	process					468:474	the WL gum biosynthesis process	444:474	the WL gum biosynthesis process	444:474	A β-1,4-glucuronosyltransferase WelK was predicted to catalyze the transfer of glucuronic acid from UDP-glucuronic acid to glucosyl-α-pyrophosphorylpolyprenol intermediate in the WL gum biosynthesis process.
32979138	4	56	theme	acid	359:362	arg1	transfer					336:343	the transfer	332:343	the transfer of glucuronic acid from UDP-glucuronic acid to glucosyl-α-pyrophosphorylpolyprenol intermediate	332:439	A β-1,4-glucuronosyltransferase WelK was predicted to catalyze the transfer of glucuronic acid from UDP-glucuronic acid to glucosyl-α-pyrophosphorylpolyprenol intermediate in the WL gum biosynthesis process.
32979138	11	57	theme	attractive	1307:1316	arg1	target					1318:1323	an attractive target	1304:1323	an attractive target for metabolic engineering	1304:1349	Thus, WelK was proved to play a critical role in the WL gum synthesis and will be an attractive target for metabolic engineering.
32979138	8	58	theme	welK	820:823	arg1	expression					806:815	Homologous expression	795:815	Homologous expression of welK in the native organism	795:846	Homologous expression of welK in the native organism can effectively improve WL gum production.
32979138	11	59	theme	WL	1275:1276	arg1	synthesis					1282:1290	the WL gum synthesis	1271:1290	the WL gum synthesis	1271:1290	Thus, WelK was proved to play a critical role in the WL gum synthesis and will be an attractive target for metabolic engineering.
32979138	12	60	theme	genes	1445:1449	arg1	characterization					1425:1440	the functional characterization	1410:1440	the functional characterization of genes in Sphingomonas sp	1410:1468	Our experiment provided a genetic manipulation method for the functional characterization of genes in Sphingomonas sp.
32979138	12	61	from	sp	1467:1468	arg1	characterization					1425:1440	the functional characterization	1410:1440	the functional characterization of genes in Sphingomonas sp	1410:1468	Our experiment provided a genetic manipulation method for the functional characterization of genes in Sphingomonas sp.
32979138	9	62	theme	wild	1064:1067	arg1	strain					1069:1074	the wild strain	1060:1074	the wild strain	1060:1074	When glucose concentration was 6.7%, the WL gum production by the welK-overexpressing strain cultured for 60 h and 84 h reached 32.65 and 43.13 g/L, 134.1%, and 114% of the wild strain.
32979138	8	63	theme	native	832:837	arg1	organism					839:846	the native organism	828:846	the native organism	828:846	Homologous expression of welK in the native organism can effectively improve WL gum production.
32979138	10	64	theme	acid	1156:1159	arg1	content					1161:1167	the glucuronic acid content	1141:1167	the glucuronic acid content	1141:1167	The polysaccharide composition and qRT-PCR analysis showed that the glucuronic acid content was closely related to the expression level of welK.
32979138	10	64	theme	acid	1156:1159	arg1	related					1181:1187	related	1181:1187	related	1181:1187	The polysaccharide composition and qRT-PCR analysis showed that the glucuronic acid content was closely related to the expression level of welK.
32979138	0	65	theme	Gum	70:72	arg1	Process					87:93	the Sphingan WL Gum Biosynthesis Process	54:93	the Sphingan WL Gum Biosynthesis Process in Marine Sphingomonas sp	54:119	The Function of β-1,4-Glucuronosyltransferase WelK in the Sphingan WL Gum Biosynthesis Process in Marine Sphingomonas sp.
32979138	5	66	theme	overexpressing	556:569	arg1	strategies					571:580	overexpressing strategies	556:580	overexpressing strategies	556:580	Its function was evaluated by bioinformatical analysis, gene knocking out, and overexpressing strategies.
32979138	8	67	theme	gum	875:877	arg1	production					879:888	WL gum production	872:888	WL gum production	872:888	Homologous expression of welK in the native organism can effectively improve WL gum production.
32979138	6	68	theme	∆welK	668:672	arg1	production					623:632	WL gum production	616:632	WL gum production	616:632	Compared to the wild strain, the WL gum production and broth viscosity of the mutant ∆welK were decreased by 71.5% and 99.2% when cultured for 48 h.
32979138	6	68	theme	∆welK	668:672	arg1	viscosity					644:652	broth viscosity	638:652	broth viscosity	638:652	Compared to the wild strain, the WL gum production and broth viscosity of the mutant ∆welK were decreased by 71.5% and 99.2% when cultured for 48 h.
32979138	10	69	theme	glucuronic	1145:1154	arg1	content					1161:1167	the glucuronic acid content	1141:1167	the glucuronic acid content	1141:1167	The polysaccharide composition and qRT-PCR analysis showed that the glucuronic acid content was closely related to the expression level of welK.
32979138	10	69	theme	glucuronic	1145:1154	arg1	related					1181:1187	related	1181:1187	related	1181:1187	The polysaccharide composition and qRT-PCR analysis showed that the glucuronic acid content was closely related to the expression level of welK.
32979138	0	70	theme	WL	67:68	arg1	Process					87:93	the Sphingan WL Gum Biosynthesis Process	54:93	the Sphingan WL Gum Biosynthesis Process in Marine Sphingomonas sp	54:119	The Function of β-1,4-Glucuronosyltransferase WelK in the Sphingan WL Gum Biosynthesis Process in Marine Sphingomonas sp.
32979138	12	71	from	characterization	1425:1440	arg1	sp					1467:1468	Sphingomonas sp	1454:1468	Sphingomonas sp	1454:1468	Our experiment provided a genetic manipulation method for the functional characterization of genes in Sphingomonas sp.
32979138	2	72	link	marine-derived	130:143	arg1	gum					163:165	The marine-derived polysaccharide WL gum	126:165	The marine-derived polysaccharide WL gum	126:165	The marine-derived polysaccharide WL gum produced by Sphingomonas sp.
32979138	6	73	theme	mutant	661:666	arg1	∆welK					668:672	the mutant ∆welK	657:672	the mutant ∆welK	657:672	Compared to the wild strain, the WL gum production and broth viscosity of the mutant ∆welK were decreased by 71.5% and 99.2% when cultured for 48 h.
32979138	7	74	theme	preparation	782:792	arg1	failure					763:769	the failure	759:769	the failure of product preparation	759:792	The gene disruption led to the failure of product preparation.
32574735	7	0	theme	crystallinity	1127:1139	arg1	degree					1117:1122	some degree	1112:1122	some degree of crystallinity and prebiotic activity	1112:1162	SP was a good biopolymer which had smooth and clearer surface with homogeneous composition, and had some degree of crystallinity and prebiotic activity.
32574735	1	1	theme	S.	348:349	arg1	seeds					361:365	Chinese S. cannabina seeds	340:365	Chinese S. cannabina seeds	340:365	The purpose of this study was to identify and determine the physical and structural characterization of the water-soluble galactomannan extracted from endosperm splits of Chinese S. cannabina seeds.
32574735	4	2	with	backbone	680:687	arg1	residues					711:718	galactopyranosyl residues	694:718	galactopyranosyl residues attached through α-(1/6) linkages	694:752	The structural data showed that SP was galactomannan which composed by a β-(1/4)-linked mannose backbone with galactopyranosyl residues attached through α-(1/6) linkages.
32574735	1	3	theme	cannabina	351:359	arg1	seeds					361:365	Chinese S. cannabina seeds	340:365	Chinese S. cannabina seeds	340:365	The purpose of this study was to identify and determine the physical and structural characterization of the water-soluble galactomannan extracted from endosperm splits of Chinese S. cannabina seeds.
32574735	7	4	theme	smooth	1047:1052	arg1	surface					1066:1072	smooth and clearer surface	1047:1072	smooth and clearer surface	1047:1072	SP was a good biopolymer which had smooth and clearer surface with homogeneous composition, and had some degree of crystallinity and prebiotic activity.
32574735	3	5	theme	molecular	485:493	arg1	structure					495:503	The molecular structure	481:503	The molecular structure of SP	481:509	The molecular structure of SP was determined by monosaccharide composition, FTIR and NMR spectroscopy.
32574735	1	6	theme	structural	242:251	arg1	characterization					253:268	the physical and structural characterization	225:268	the physical and structural characterization of the water-soluble galactomannan extracted from endosperm splits of Chinese S. cannabina seeds	225:365	The purpose of this study was to identify and determine the physical and structural characterization of the water-soluble galactomannan extracted from endosperm splits of Chinese S. cannabina seeds.
32574735	0	7	theme	galactomannan	86:98	arg1	activity					72:79	in vitro prebiotic activity	53:79	in vitro prebiotic activity	53:79	Isolation, physical, structural characterization and in vitro prebiotic activity of a galactomannan extracted from endosperm splits of Chinese Sesbania cannabina seeds.
32574735	0	7	theme	galactomannan	86:98	arg1	characterization					32:47	physical, structural characterization	11:47	physical, structural characterization	11:47	Isolation, physical, structural characterization and in vitro prebiotic activity of a galactomannan extracted from endosperm splits of Chinese Sesbania cannabina seeds.
32574735	0	7	theme	galactomannan	86:98	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, physical, structural characterization and in vitro prebiotic activity of a galactomannan extracted from endosperm splits of Chinese Sesbania cannabina seeds.
32574735	1	8	theme	seeds	361:365	arg1	endosperm					320:328	endosperm splits	320:335	endosperm splits of Chinese S. cannabina seeds	320:365	The purpose of this study was to identify and determine the physical and structural characterization of the water-soluble galactomannan extracted from endosperm splits of Chinese S. cannabina seeds.
32574735	7	9	contain	had	1043:1045	arg1	SP					1012:1013	SP	1012:1013	SP	1012:1013	SP was a good biopolymer which had smooth and clearer surface with homogeneous composition, and had some degree of crystallinity and prebiotic activity.
32574735	7	9	contain	had	1043:1045	arg1	biopolymer					1026:1035	a good biopolymer	1019:1035	a good biopolymer which had smooth and clearer surface with homogeneous composition, and had some degree of crystallinity and prebiotic activity	1019:1162	SP was a good biopolymer which had smooth and clearer surface with homogeneous composition, and had some degree of crystallinity and prebiotic activity.
32574735	7	9	contain	had	1043:1045	arg2	surface					1066:1072	smooth and clearer surface	1047:1072	smooth and clearer surface	1047:1072	SP was a good biopolymer which had smooth and clearer surface with homogeneous composition, and had some degree of crystallinity and prebiotic activity.
32574735	6	10	theme	many	926:929	arg1	branches					931:938	many branches	926:938	many branches	926:938	The physical results revealed that SP had many branches on the backbone and existed as a random coil state in aqueous solution.
32574735	8	11	theme	potential	1197:1205	arg1	prebiotic					1207:1215	a potential prebiotic	1195:1215	a potential prebiotic	1195:1215	As a consequence, SP could be a potential prebiotic and was expected to be suitable for applications in food, pharmaceutical, biomedical or cosmetic industries as a promising new biomaterial.
32574735	8	11	theme	potential	1197:1205	arg1	SP					1183:1184	SP	1183:1184	SP	1183:1184	As a consequence, SP could be a potential prebiotic and was expected to be suitable for applications in food, pharmaceutical, biomedical or cosmetic industries as a promising new biomaterial.
32574735	3	12	theme	SP	508:509	arg1	structure					495:503	The molecular structure	481:503	The molecular structure of SP	481:509	The molecular structure of SP was determined by monosaccharide composition, FTIR and NMR spectroscopy.
32574735	7	13	theme	clearer	1058:1064	arg1	surface					1066:1072	smooth and clearer surface	1047:1072	smooth and clearer surface	1047:1072	SP was a good biopolymer which had smooth and clearer surface with homogeneous composition, and had some degree of crystallinity and prebiotic activity.
32574735	7	14	theme	good	1021:1024	arg1	SP					1012:1013	SP	1012:1013	SP	1012:1013	SP was a good biopolymer which had smooth and clearer surface with homogeneous composition, and had some degree of crystallinity and prebiotic activity.
32574735	7	14	theme	good	1021:1024	arg1	biopolymer					1026:1035	a good biopolymer	1019:1035	a good biopolymer which had smooth and clearer surface with homogeneous composition, and had some degree of crystallinity and prebiotic activity	1019:1162	SP was a good biopolymer which had smooth and clearer surface with homogeneous composition, and had some degree of crystallinity and prebiotic activity.
32574735	4	15	link	-linked	664:670	arg1	backbone					680:687	a β-(1/4)-linked mannose backbone	655:687	a β-(1/4)-linked mannose backbone with galactopyranosyl residues attached through α-(1/6) linkages	655:752	The structural data showed that SP was galactomannan which composed by a β-(1/4)-linked mannose backbone with galactopyranosyl residues attached through α-(1/6) linkages.
32574735	5	16	theme	mannose/galactose	768:784	arg1	ratio					792:796	The constant mannose/galactose (M/G) ratio	755:796	The constant mannose/galactose (M/G) ratio	755:796	The constant mannose/galactose (M/G) ratio and average molecular weight (Mw) of SP was 1.6:1 and 2.16 × 105 g/mol, respectively.
32574735	5	16	theme	mannose/galactose	768:784	arg1	1.6:1					842:846	1.6:1	842:846	1.6:1	842:846	The constant mannose/galactose (M/G) ratio and average molecular weight (Mw) of SP was 1.6:1 and 2.16 × 105 g/mol, respectively.
32574735	1	17	dep	endosperm	320:328	arg1	splits					330:335	splits	330:335	splits	330:335	The purpose of this study was to identify and determine the physical and structural characterization of the water-soluble galactomannan extracted from endosperm splits of Chinese S. cannabina seeds.
32574735	7	18	theme	prebiotic	1145:1153	arg1	activity					1155:1162	prebiotic activity	1145:1162	prebiotic activity	1145:1162	SP was a good biopolymer which had smooth and clearer surface with homogeneous composition, and had some degree of crystallinity and prebiotic activity.
32574735	6	19	theme	aqueous	994:1000	arg1	solution					1002:1009	aqueous solution	994:1009	aqueous solution	994:1009	The physical results revealed that SP had many branches on the backbone and existed as a random coil state in aqueous solution.
32574735	6	20	from	state	985:989	arg1	solution					1002:1009	aqueous solution	994:1009	aqueous solution	994:1009	The physical results revealed that SP had many branches on the backbone and existed as a random coil state in aqueous solution.
32574735	4	21	theme	galactopyranosyl	694:709	arg1	residues					711:718	galactopyranosyl residues	694:718	galactopyranosyl residues attached through α-(1/6) linkages	694:752	The structural data showed that SP was galactomannan which composed by a β-(1/4)-linked mannose backbone with galactopyranosyl residues attached through α-(1/6) linkages.
32574735	2	22	theme	novel	435:439	arg1	method					441:446	a novel method	433:446	a novel method with a high yield (40.3 ± 7.2%)	433:478	The Sesbania galactomannan (SP) was extracted and purified using a novel method with a high yield (40.3 ± 7.2%).
32574735	1	23	theme	water-soluble	277:289	arg1	galactomannan					291:303	the water-soluble galactomannan	273:303	the water-soluble galactomannan extracted from endosperm splits of Chinese S. cannabina seeds	273:365	The purpose of this study was to identify and determine the physical and structural characterization of the water-soluble galactomannan extracted from endosperm splits of Chinese S. cannabina seeds.
32574735	6	24	contain	had	922:924	arg1	SP					919:920	SP	919:920	SP	919:920	The physical results revealed that SP had many branches on the backbone and existed as a random coil state in aqueous solution.
32574735	6	24	contain	had	922:924	arg2	branches					931:938	many branches	926:938	many branches	926:938	The physical results revealed that SP had many branches on the backbone and existed as a random coil state in aqueous solution.
32574735	3	25	theme	monosaccharide	529:542	arg1	composition					544:554	monosaccharide composition	529:554	monosaccharide composition	529:554	The molecular structure of SP was determined by monosaccharide composition, FTIR and NMR spectroscopy.
32574735	1	26	theme	study	189:193	arg1	purpose					173:179	The purpose	169:179	The purpose of this study	169:193	The purpose of this study was to identify and determine the physical and structural characterization of the water-soluble galactomannan extracted from endosperm splits of Chinese S. cannabina seeds.
32574735	0	27	theme	physical	11:18	arg1	characterization					32:47	physical, structural characterization	11:47	physical, structural characterization	11:47	Isolation, physical, structural characterization and in vitro prebiotic activity of a galactomannan extracted from endosperm splits of Chinese Sesbania cannabina seeds.
32574735	1	28	theme	galactomannan	291:303	arg1	characterization					253:268	the physical and structural characterization	225:268	the physical and structural characterization of the water-soluble galactomannan extracted from endosperm splits of Chinese S. cannabina seeds	225:365	The purpose of this study was to identify and determine the physical and structural characterization of the water-soluble galactomannan extracted from endosperm splits of Chinese S. cannabina seeds.
32574735	8	29	theme	biomedical	1291:1300	arg1	industries					1314:1323	food, pharmaceutical, biomedical or cosmetic industries	1269:1323	food, pharmaceutical, biomedical or cosmetic industries as a promising new biomaterial	1269:1354	As a consequence, SP could be a potential prebiotic and was expected to be suitable for applications in food, pharmaceutical, biomedical or cosmetic industries as a promising new biomaterial.
32574735	8	30	theme	pharmaceutical	1275:1288	arg1	industries					1314:1323	food, pharmaceutical, biomedical or cosmetic industries	1269:1323	food, pharmaceutical, biomedical or cosmetic industries as a promising new biomaterial	1269:1354	As a consequence, SP could be a potential prebiotic and was expected to be suitable for applications in food, pharmaceutical, biomedical or cosmetic industries as a promising new biomaterial.
32574735	6	31	theme	coil	980:983	arg1	state					985:989	a random coil state	971:989	a random coil state in aqueous solution	971:1009	The physical results revealed that SP had many branches on the backbone and existed as a random coil state in aqueous solution.
32574735	0	32	theme	Chinese	135:141	arg1	seeds					162:166	Chinese Sesbania cannabina seeds	135:166	Chinese Sesbania cannabina seeds	135:166	Isolation, physical, structural characterization and in vitro prebiotic activity of a galactomannan extracted from endosperm splits of Chinese Sesbania cannabina seeds.
32574735	6	33	theme	random	973:978	arg1	state					985:989	a random coil state	971:989	a random coil state in aqueous solution	971:1009	The physical results revealed that SP had many branches on the backbone and existed as a random coil state in aqueous solution.
32574735	0	34	dep	in	53:54	arg1	vitro					56:60	vitro	56:60	vitro	56:60	Isolation, physical, structural characterization and in vitro prebiotic activity of a galactomannan extracted from endosperm splits of Chinese Sesbania cannabina seeds.
32574735	5	35	theme	average	802:808	arg1	Mw					828:829	Mw	828:829	Mw	828:829	The constant mannose/galactose (M/G) ratio and average molecular weight (Mw) of SP was 1.6:1 and 2.16 × 105 g/mol, respectively.
32574735	5	35	theme	average	802:808	arg1	weight					820:825	average molecular weight	802:825	average molecular weight (Mw)	802:830	The constant mannose/galactose (M/G) ratio and average molecular weight (Mw) of SP was 1.6:1 and 2.16 × 105 g/mol, respectively.
32574735	5	36	theme	M/G	787:789	arg1	ratio					792:796	The constant mannose/galactose (M/G) ratio	755:796	The constant mannose/galactose (M/G) ratio	755:796	The constant mannose/galactose (M/G) ratio and average molecular weight (Mw) of SP was 1.6:1 and 2.16 × 105 g/mol, respectively.
32574735	5	36	theme	M/G	787:789	arg1	1.6:1					842:846	1.6:1	842:846	1.6:1	842:846	The constant mannose/galactose (M/G) ratio and average molecular weight (Mw) of SP was 1.6:1 and 2.16 × 105 g/mol, respectively.
32574735	5	37	theme	molecular	810:818	arg1	Mw					828:829	Mw	828:829	Mw	828:829	The constant mannose/galactose (M/G) ratio and average molecular weight (Mw) of SP was 1.6:1 and 2.16 × 105 g/mol, respectively.
32574735	5	37	theme	molecular	810:818	arg1	weight					820:825	average molecular weight	802:825	average molecular weight (Mw)	802:830	The constant mannose/galactose (M/G) ratio and average molecular weight (Mw) of SP was 1.6:1 and 2.16 × 105 g/mol, respectively.
32574735	2	38	theme	high	455:458	arg1	yield					460:464	a high yield	453:464	a high yield (40.3 ± 7.2%)	453:478	The Sesbania galactomannan (SP) was extracted and purified using a novel method with a high yield (40.3 ± 7.2%).
32574735	2	38	theme	high	455:458	arg1	%					477:477	40.3 ± 7.2%	467:477	40.3 ± 7.2%	467:477	The Sesbania galactomannan (SP) was extracted and purified using a novel method with a high yield (40.3 ± 7.2%).
32574735	6	39	theme	physical	888:895	arg1	results					897:903	The physical results	884:903	The physical results	884:903	The physical results revealed that SP had many branches on the backbone and existed as a random coil state in aqueous solution.
32574735	0	40	theme	cannabina	152:160	arg1	seeds					162:166	Chinese Sesbania cannabina seeds	135:166	Chinese Sesbania cannabina seeds	135:166	Isolation, physical, structural characterization and in vitro prebiotic activity of a galactomannan extracted from endosperm splits of Chinese Sesbania cannabina seeds.
32574735	7	41	theme	activity	1155:1162	arg1	degree					1117:1122	some degree	1112:1122	some degree of crystallinity and prebiotic activity	1112:1162	SP was a good biopolymer which had smooth and clearer surface with homogeneous composition, and had some degree of crystallinity and prebiotic activity.
32574735	7	42	contain	had	1108:1110	arg1	SP					1012:1013	SP	1012:1013	SP	1012:1013	SP was a good biopolymer which had smooth and clearer surface with homogeneous composition, and had some degree of crystallinity and prebiotic activity.
32574735	7	42	contain	had	1108:1110	arg1	biopolymer					1026:1035	a good biopolymer	1019:1035	a good biopolymer which had smooth and clearer surface with homogeneous composition, and had some degree of crystallinity and prebiotic activity	1019:1162	SP was a good biopolymer which had smooth and clearer surface with homogeneous composition, and had some degree of crystallinity and prebiotic activity.
32574735	7	42	contain	had	1108:1110	arg2	degree					1117:1122	some degree	1112:1122	some degree of crystallinity and prebiotic activity	1112:1162	SP was a good biopolymer which had smooth and clearer surface with homogeneous composition, and had some degree of crystallinity and prebiotic activity.
32574735	4	43	theme	mannose	672:678	arg1	backbone					680:687	a β-(1/4)-linked mannose backbone	655:687	a β-(1/4)-linked mannose backbone with galactopyranosyl residues attached through α-(1/6) linkages	655:752	The structural data showed that SP was galactomannan which composed by a β-(1/4)-linked mannose backbone with galactopyranosyl residues attached through α-(1/6) linkages.
32574735	5	44	theme	constant	759:766	arg1	ratio					792:796	The constant mannose/galactose (M/G) ratio	755:796	The constant mannose/galactose (M/G) ratio	755:796	The constant mannose/galactose (M/G) ratio and average molecular weight (Mw) of SP was 1.6:1 and 2.16 × 105 g/mol, respectively.
32574735	5	44	theme	constant	759:766	arg1	1.6:1					842:846	1.6:1	842:846	1.6:1	842:846	The constant mannose/galactose (M/G) ratio and average molecular weight (Mw) of SP was 1.6:1 and 2.16 × 105 g/mol, respectively.
32574735	0	45	theme	Sesbania	143:150	arg1	seeds					162:166	Chinese Sesbania cannabina seeds	135:166	Chinese Sesbania cannabina seeds	135:166	Isolation, physical, structural characterization and in vitro prebiotic activity of a galactomannan extracted from endosperm splits of Chinese Sesbania cannabina seeds.
32574735	3	46	theme	NMR	566:568	arg1	spectroscopy					570:581	NMR spectroscopy	566:581	NMR spectroscopy	566:581	The molecular structure of SP was determined by monosaccharide composition, FTIR and NMR spectroscopy.
32574735	4	47	theme	-linked	664:670	arg1	backbone					680:687	a β-(1/4)-linked mannose backbone	655:687	a β-(1/4)-linked mannose backbone with galactopyranosyl residues attached through α-(1/6) linkages	655:752	The structural data showed that SP was galactomannan which composed by a β-(1/4)-linked mannose backbone with galactopyranosyl residues attached through α-(1/6) linkages.
32574735	7	48	theme	homogeneous	1079:1089	arg1	composition					1091:1101	homogeneous composition	1079:1101	homogeneous composition	1079:1101	SP was a good biopolymer which had smooth and clearer surface with homogeneous composition, and had some degree of crystallinity and prebiotic activity.
32574735	8	49	theme	food	1269:1272	arg1	industries					1314:1323	food, pharmaceutical, biomedical or cosmetic industries	1269:1323	food, pharmaceutical, biomedical or cosmetic industries as a promising new biomaterial	1269:1354	As a consequence, SP could be a potential prebiotic and was expected to be suitable for applications in food, pharmaceutical, biomedical or cosmetic industries as a promising new biomaterial.
32574735	2	50	with	method	441:446	arg1	yield					460:464	a high yield	453:464	a high yield (40.3 ± 7.2%)	453:478	The Sesbania galactomannan (SP) was extracted and purified using a novel method with a high yield (40.3 ± 7.2%).
32574735	2	50	with	method	441:446	arg1	%					477:477	40.3 ± 7.2%	467:477	40.3 ± 7.2%	467:477	The Sesbania galactomannan (SP) was extracted and purified using a novel method with a high yield (40.3 ± 7.2%).
32574735	8	51	from	applications	1253:1264	arg1	industries					1314:1323	food, pharmaceutical, biomedical or cosmetic industries	1269:1323	food, pharmaceutical, biomedical or cosmetic industries as a promising new biomaterial	1269:1354	As a consequence, SP could be a potential prebiotic and was expected to be suitable for applications in food, pharmaceutical, biomedical or cosmetic industries as a promising new biomaterial.
32574735	4	52	theme	structural	588:597	arg1	data					599:602	The structural data	584:602	The structural data	584:602	The structural data showed that SP was galactomannan which composed by a β-(1/4)-linked mannose backbone with galactopyranosyl residues attached through α-(1/6) linkages.
32574735	0	53	dep	physical	11:18	arg1	structural					21:30	structural	21:30	structural	21:30	Isolation, physical, structural characterization and in vitro prebiotic activity of a galactomannan extracted from endosperm splits of Chinese Sesbania cannabina seeds.
32574735	5	54	theme	SP	835:836	arg1	ratio					792:796	The constant mannose/galactose (M/G) ratio	755:796	The constant mannose/galactose (M/G) ratio	755:796	The constant mannose/galactose (M/G) ratio and average molecular weight (Mw) of SP was 1.6:1 and 2.16 × 105 g/mol, respectively.
32574735	5	54	theme	SP	835:836	arg1	1.6:1					842:846	1.6:1	842:846	1.6:1	842:846	The constant mannose/galactose (M/G) ratio and average molecular weight (Mw) of SP was 1.6:1 and 2.16 × 105 g/mol, respectively.
32574735	5	54	theme	SP	835:836	arg1	Mw					828:829	Mw	828:829	Mw	828:829	The constant mannose/galactose (M/G) ratio and average molecular weight (Mw) of SP was 1.6:1 and 2.16 × 105 g/mol, respectively.
32574735	5	54	theme	SP	835:836	arg1	weight					820:825	average molecular weight	802:825	average molecular weight (Mw)	802:830	The constant mannose/galactose (M/G) ratio and average molecular weight (Mw) of SP was 1.6:1 and 2.16 × 105 g/mol, respectively.
32574735	0	55	theme	prebiotic	62:70	arg1	activity					72:79	in vitro prebiotic activity	53:79	in vitro prebiotic activity	53:79	Isolation, physical, structural characterization and in vitro prebiotic activity of a galactomannan extracted from endosperm splits of Chinese Sesbania cannabina seeds.
32574735	8	56	theme	new	1340:1342	arg1	biomaterial					1344:1354	a promising new biomaterial	1328:1354	a promising new biomaterial	1328:1354	As a consequence, SP could be a potential prebiotic and was expected to be suitable for applications in food, pharmaceutical, biomedical or cosmetic industries as a promising new biomaterial.
32574735	8	57	theme	cosmetic	1305:1312	arg1	industries					1314:1323	food, pharmaceutical, biomedical or cosmetic industries	1269:1323	food, pharmaceutical, biomedical or cosmetic industries as a promising new biomaterial	1269:1354	As a consequence, SP could be a potential prebiotic and was expected to be suitable for applications in food, pharmaceutical, biomedical or cosmetic industries as a promising new biomaterial.
32574735	0	58	theme	in	53:54	arg1	activity					72:79	in vitro prebiotic activity	53:79	in vitro prebiotic activity	53:79	Isolation, physical, structural characterization and in vitro prebiotic activity of a galactomannan extracted from endosperm splits of Chinese Sesbania cannabina seeds.
32574735	1	59	theme	Chinese	340:346	arg1	seeds					361:365	Chinese S. cannabina seeds	340:365	Chinese S. cannabina seeds	340:365	The purpose of this study was to identify and determine the physical and structural characterization of the water-soluble galactomannan extracted from endosperm splits of Chinese S. cannabina seeds.
32574735	8	60	theme	promising	1330:1338	arg1	biomaterial					1344:1354	a promising new biomaterial	1328:1354	a promising new biomaterial	1328:1354	As a consequence, SP could be a potential prebiotic and was expected to be suitable for applications in food, pharmaceutical, biomedical or cosmetic industries as a promising new biomaterial.
32574735	2	61	theme	Sesbania	372:379	arg1	SP					396:397	SP	396:397	SP	396:397	The Sesbania galactomannan (SP) was extracted and purified using a novel method with a high yield (40.3 ± 7.2%).
32574735	2	61	theme	Sesbania	372:379	arg1	galactomannan					381:393	The Sesbania galactomannan	368:393	The Sesbania galactomannan (SP)	368:398	The Sesbania galactomannan (SP) was extracted and purified using a novel method with a high yield (40.3 ± 7.2%).
32574735	1	62	theme	physical	229:236	arg1	characterization					253:268	the physical and structural characterization	225:268	the physical and structural characterization of the water-soluble galactomannan extracted from endosperm splits of Chinese S. cannabina seeds	225:365	The purpose of this study was to identify and determine the physical and structural characterization of the water-soluble galactomannan extracted from endosperm splits of Chinese S. cannabina seeds.
33465610	3	0	theme	temperature	475:485	arg1	effects					376:382	The effects	372:382	The effects of the initial pH, Cr concentration, adsorbent dosage, contact time, composition ratio and temperature on adsorption capacity	372:508	The effects of the initial pH, Cr concentration, adsorbent dosage, contact time, composition ratio and temperature on adsorption capacity were explored.
33465610	8	1	from	removal	1292:1298	arg1	solution					1346:1353	aqueous solution	1338:1353	aqueous solution	1338:1353	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	8	2	theme	Synergic	1096:1103	arg1	effect					1105:1110	Synergic effect	1096:1110	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange	1096:1191	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	10	3	theme	metal	1672:1676	arg1	pollution					1678:1686	heavy metal pollution	1666:1686	heavy metal pollution control	1666:1694	This study provides a reasonable scheme for the targeted use of waste biomass for heavy metal pollution control.
33465610	9	4	dep	transform	1506:1514	arg1	infrared					1516:1523	infrared	1516:1523	transform infrared spectrometry and X-ray photoelectron spectroscopy results	1506:1581	Cr(VI) anions can be chelated by oxygen-containing functional groups on SCB surface after reduction to simple cations, which was confirmed by Fourier transform infrared spectrometry and X-ray photoelectron spectroscopy results.
33465610	7	5	theme	Langmuir	1026:1033	arg1	model					1044:1048	the Langmuir isotherm model	1022:1048	the Langmuir isotherm model with the adsorption capacity of 156-251 mg/g	1022:1093	The adsorption isotherm data were consistent with the Langmuir isotherm model with the adsorption capacity of 156-251 mg/g.
33465610	9	6	from	groups	1418:1423	arg1	surface					1432:1438	SCB surface	1428:1438	SCB surface	1428:1438	Cr(VI) anions can be chelated by oxygen-containing functional groups on SCB surface after reduction to simple cations, which was confirmed by Fourier transform infrared spectrometry and X-ray photoelectron spectroscopy results.
33465610	8	7	theme	exchange	1184:1191	arg1	processes					1138:1146	the processes	1134:1146	the processes of electrostatic adsorption and ion exchange	1134:1191	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	2	8	theme	Cr	310:311	arg1	adsorption					282:291	efficient adsorption	272:291	efficient adsorption	272:291	Polypyrrole/sugarcane bagasse composites (PPy/SCB) for efficient adsorption and reduction of Cr(VI) in wastewater were prepared by in-situ polymerization.
33465610	2	8	theme	Cr	310:311	arg1	reduction					297:305	reduction	297:305	reduction of Cr(VI) in wastewater	297:329	Polypyrrole/sugarcane bagasse composites (PPy/SCB) for efficient adsorption and reduction of Cr(VI) in wastewater were prepared by in-situ polymerization.
33465610	8	9	theme	in-situ	1194:1200	arg1	reduction					1202:1210	in-situ reduction	1194:1210	in-situ reduction of electron donors	1194:1229	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	9	10	dep	spectrometry	1525:1536	arg1	results					1575:1581	results	1575:1581	results	1575:1581	Cr(VI) anions can be chelated by oxygen-containing functional groups on SCB surface after reduction to simple cations, which was confirmed by Fourier transform infrared spectrometry and X-ray photoelectron spectroscopy results.
33465610	7	11	theme	isotherm	987:994	arg1	consistent					1006:1015	consistent	1006:1015	consistent	1006:1015	The adsorption isotherm data were consistent with the Langmuir isotherm model with the adsorption capacity of 156-251 mg/g.
33465610	7	11	theme	isotherm	987:994	arg1	data					996:999	The adsorption isotherm data	972:999	The adsorption isotherm data	972:999	The adsorption isotherm data were consistent with the Langmuir isotherm model with the adsorption capacity of 156-251 mg/g.
33465610	5	12	theme	PPy/SCB	775:781	arg1	surface					783:789	the PPy/SCB surface	771:789	the PPy/SCB surface	771:789	The adsorbents before and after adsorption were characterized, which confirmed that adsorption process affects the morphology, composition and structure of the PPy/SCB surface.
33465610	1	13	theme	sustainable	192:202	arg1	development					204:214	sustainable development	192:214	sustainable development	192:214	Reduction and recycling of Cr(VI) can offer environmental governance and sustainable development.
33465610	8	14	theme	electrostatic	1151:1163	arg1	adsorption					1165:1174	electrostatic adsorption	1151:1174	electrostatic adsorption	1151:1174	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	8	15	theme	donors	1224:1229	arg1	reduction					1202:1210	in-situ reduction	1194:1210	in-situ reduction of electron donors	1194:1229	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	8	15	theme	donors	1224:1229	arg1	effect					1105:1110	Synergic effect	1096:1110	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange	1096:1191	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	8	15	theme	donors	1224:1229	arg1	coordination					1236:1247	coordination	1236:1247	coordination	1236:1247	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	10	16	theme	heavy	1666:1670	arg1	pollution					1678:1686	heavy metal pollution	1666:1686	heavy metal pollution control	1666:1694	This study provides a reasonable scheme for the targeted use of waste biomass for heavy metal pollution control.
33465610	9	17	theme	oxygen-containing	1389:1405	arg1	groups					1418:1423	oxygen-containing functional groups	1389:1423	oxygen-containing functional groups on SCB surface	1389:1438	Cr(VI) anions can be chelated by oxygen-containing functional groups on SCB surface after reduction to simple cations, which was confirmed by Fourier transform infrared spectrometry and X-ray photoelectron spectroscopy results.
33465610	0	18	theme	in-situ	31:37	arg1	reduction					51:59	in-situ synergistic reduction	31:59	in-situ synergistic reduction	31:59	Enhanced removal of Cr(VI) via in-situ synergistic reduction and fixation by polypyrrole/sugarcane bagasse composites.
33465610	9	19	theme	SCB	1428:1430	arg1	surface					1432:1438	SCB surface	1428:1438	SCB surface	1428:1438	Cr(VI) anions can be chelated by oxygen-containing functional groups on SCB surface after reduction to simple cations, which was confirmed by Fourier transform infrared spectrometry and X-ray photoelectron spectroscopy results.
33465610	8	20	attach	removal	1292:1298	arg2	Cr					1332:1333	total Cr	1326:1333	total Cr	1326:1333	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	8	20	attach	removal	1292:1298	arg3	solution					1346:1353	aqueous solution	1338:1353	aqueous solution	1338:1353	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	3	21	theme	concentration	406:418	arg1	effects					376:382	The effects	372:382	The effects of the initial pH, Cr concentration, adsorbent dosage, contact time, composition ratio and temperature on adsorption capacity	372:508	The effects of the initial pH, Cr concentration, adsorbent dosage, contact time, composition ratio and temperature on adsorption capacity were explored.
33465610	5	22	theme	adsorption	699:708	arg1	process					710:716	adsorption process	699:716	adsorption process	699:716	The adsorbents before and after adsorption were characterized, which confirmed that adsorption process affects the morphology, composition and structure of the PPy/SCB surface.
33465610	1	23	dep	Cr	146:147	arg1	VI					149:150	VI	149:150	VI	149:150	Reduction and recycling of Cr(VI) can offer environmental governance and sustainable development.
33465610	8	24	theme	toxicity	1304:1311	arg1	reduction					1313:1321	toxicity reduction	1304:1321	toxicity reduction of total Cr in aqueous solution	1304:1353	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	10	25	theme	reasonable	1606:1615	arg1	scheme					1617:1622	a reasonable scheme	1604:1622	a reasonable scheme for the targeted use of waste biomass	1604:1660	This study provides a reasonable scheme for the targeted use of waste biomass for heavy metal pollution control.
33465610	5	26	theme	surface	783:789	arg1	structure					758:766	structure	758:766	structure	758:766	The adsorbents before and after adsorption were characterized, which confirmed that adsorption process affects the morphology, composition and structure of the PPy/SCB surface.
33465610	5	26	theme	surface	783:789	arg1	composition					742:752	composition	742:752	composition	742:752	The adsorbents before and after adsorption were characterized, which confirmed that adsorption process affects the morphology, composition and structure of the PPy/SCB surface.
33465610	5	26	theme	surface	783:789	arg1	morphology					730:739	morphology	730:739	morphology	730:739	The adsorbents before and after adsorption were characterized, which confirmed that adsorption process affects the morphology, composition and structure of the PPy/SCB surface.
33465610	10	27	theme	biomass	1654:1660	arg1	use					1641:1643	the targeted use	1628:1643	the targeted use of waste biomass	1628:1660	This study provides a reasonable scheme for the targeted use of waste biomass for heavy metal pollution control.
33465610	3	28	theme	initial	391:397	arg1	pH					399:400	the initial pH	387:400	the initial pH	387:400	The effects of the initial pH, Cr concentration, adsorbent dosage, contact time, composition ratio and temperature on adsorption capacity were explored.
33465610	3	29	theme	adsorbent	421:429	arg1	dosage					431:436	adsorbent dosage	421:436	adsorbent dosage	421:436	The effects of the initial pH, Cr concentration, adsorbent dosage, contact time, composition ratio and temperature on adsorption capacity were explored.
33465610	7	30	theme	156-251 mg/g	1082:1093	arg1	capacity					1070:1077	the adsorption capacity	1055:1077	the adsorption capacity of 156-251 mg/g	1055:1093	The adsorption isotherm data were consistent with the Langmuir isotherm model with the adsorption capacity of 156-251 mg/g.
33465610	8	31	theme	Cr	1332:1333	arg1	removal					1292:1298	the rapid removal	1282:1298	the rapid removal	1282:1298	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	8	31	theme	Cr	1332:1333	arg1	reduction					1313:1321	toxicity reduction	1304:1321	toxicity reduction of total Cr in aqueous solution	1304:1353	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	3	32	theme	composition	453:463	arg1	ratio					465:469	composition ratio	453:469	composition ratio	453:469	The effects of the initial pH, Cr concentration, adsorbent dosage, contact time, composition ratio and temperature on adsorption capacity were explored.
33465610	6	33	theme	kinetics	807:814	arg1	data					816:819	The adsorption kinetics data	792:819	The adsorption kinetics data of Cr(VI)	792:829	The adsorption kinetics data of Cr(VI) were best fitted by the pseudo-second-order model, which indicated that Cr(VI) concentration gradient played a driving role in this process.
33465610	6	34	dep	Cr	824:825	arg1	VI					827:828	VI	827:828	VI	827:828	The adsorption kinetics data of Cr(VI) were best fitted by the pseudo-second-order model, which indicated that Cr(VI) concentration gradient played a driving role in this process.
33465610	8	35	theme	aqueous	1338:1344	arg1	solution					1346:1353	aqueous solution	1338:1353	aqueous solution	1338:1353	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	3	36	theme	time	447:450	arg1	effects					376:382	The effects	372:382	The effects of the initial pH, Cr concentration, adsorbent dosage, contact time, composition ratio and temperature on adsorption capacity	372:508	The effects of the initial pH, Cr concentration, adsorbent dosage, contact time, composition ratio and temperature on adsorption capacity were explored.
33465610	3	37	theme	dosage	431:436	arg1	effects					376:382	The effects	372:382	The effects of the initial pH, Cr concentration, adsorbent dosage, contact time, composition ratio and temperature on adsorption capacity	372:508	The effects of the initial pH, Cr concentration, adsorbent dosage, contact time, composition ratio and temperature on adsorption capacity were explored.
33465610	2	38	theme	in-situ	348:354	arg1	polymerization					356:369	in-situ polymerization	348:369	in-situ polymerization	348:369	Polypyrrole/sugarcane bagasse composites (PPy/SCB) for efficient adsorption and reduction of Cr(VI) in wastewater were prepared by in-situ polymerization.
33465610	6	39	theme	pseudo-second-order	855:873	arg1	model					875:879	the pseudo-second-order model	851:879	the pseudo-second-order model	851:879	The adsorption kinetics data of Cr(VI) were best fitted by the pseudo-second-order model, which indicated that Cr(VI) concentration gradient played a driving role in this process.
33465610	9	40	theme	photoelectron	1548:1560	arg1	spectroscopy					1562:1573	X-ray photoelectron spectroscopy	1542:1573	X-ray photoelectron spectroscopy	1542:1573	Cr(VI) anions can be chelated by oxygen-containing functional groups on SCB surface after reduction to simple cations, which was confirmed by Fourier transform infrared spectrometry and X-ray photoelectron spectroscopy results.
33465610	7	41	with	model	1044:1048	arg1	capacity					1070:1077	the adsorption capacity	1055:1077	the adsorption capacity of 156-251 mg/g	1055:1093	The adsorption isotherm data were consistent with the Langmuir isotherm model with the adsorption capacity of 156-251 mg/g.
33465610	7	42	theme	isotherm	1035:1042	arg1	model					1044:1048	the Langmuir isotherm model	1022:1048	the Langmuir isotherm model with the adsorption capacity of 156-251 mg/g	1022:1093	The adsorption isotherm data were consistent with the Langmuir isotherm model with the adsorption capacity of 156-251 mg/g.
33465610	9	43	dep	Cr	1356:1357	arg1	VI					1359:1360	VI	1359:1360	VI	1359:1360	Cr(VI) anions can be chelated by oxygen-containing functional groups on SCB surface after reduction to simple cations, which was confirmed by Fourier transform infrared spectrometry and X-ray photoelectron spectroscopy results.
33465610	9	43	dep	Cr	1356:1357	arg1	anions					1363:1368	anions	1363:1368	Cr(VI) anions	1356:1368	Cr(VI) anions can be chelated by oxygen-containing functional groups on SCB surface after reduction to simple cations, which was confirmed by Fourier transform infrared spectrometry and X-ray photoelectron spectroscopy results.
33465610	10	44	theme	pollution	1678:1686	arg1	control					1688:1694	heavy metal pollution control	1666:1694	heavy metal pollution control	1666:1694	This study provides a reasonable scheme for the targeted use of waste biomass for heavy metal pollution control.
33465610	4	45	theme	adsorption	544:553	arg1	capacity					555:562	adsorption capacity	544:562	The optimal Cr(VI) adsorption capacity of PPy/SCB	525:573	The optimal Cr(VI) adsorption capacity of PPy/SCB was achieved when the pH value was 2.0.
33465610	10	46	theme	targeted	1632:1639	arg1	use					1641:1643	the targeted use	1628:1643	the targeted use of waste biomass	1628:1660	This study provides a reasonable scheme for the targeted use of waste biomass for heavy metal pollution control.
33465610	2	47	theme	efficient	272:280	arg1	adsorption					282:291	efficient adsorption	272:291	efficient adsorption	272:291	Polypyrrole/sugarcane bagasse composites (PPy/SCB) for efficient adsorption and reduction of Cr(VI) in wastewater were prepared by in-situ polymerization.
33465610	8	48	theme	adsorption	1165:1174	arg1	processes					1138:1146	the processes	1134:1146	the processes of electrostatic adsorption and ion exchange	1134:1191	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	8	49	from	reduction	1313:1321	arg1	solution					1346:1353	aqueous solution	1338:1353	aqueous solution	1338:1353	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	8	50	theme	PPy	1115:1117	arg1	reduction					1202:1210	in-situ reduction	1194:1210	in-situ reduction of electron donors	1194:1229	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	8	50	theme	PPy	1115:1117	arg1	effect					1105:1110	Synergic effect	1096:1110	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange	1096:1191	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	8	50	theme	PPy	1115:1117	arg1	coordination					1236:1247	coordination	1236:1247	coordination	1236:1247	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	8	51	theme	ion	1180:1182	arg1	exchange					1184:1191	ion exchange	1180:1191	ion exchange	1180:1191	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	1	52	theme	environmental	163:175	arg1	governance					177:186	environmental governance	163:186	environmental governance	163:186	Reduction and recycling of Cr(VI) can offer environmental governance and sustainable development.
33465610	8	53	theme	SCB	1123:1125	arg1	reduction					1202:1210	in-situ reduction	1194:1210	in-situ reduction of electron donors	1194:1229	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	8	53	theme	SCB	1123:1125	arg1	effect					1105:1110	Synergic effect	1096:1110	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange	1096:1191	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	8	53	theme	SCB	1123:1125	arg1	coordination					1236:1247	coordination	1236:1247	coordination	1236:1247	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	5	54	dep	morphology	730:739	arg1	the					726:728	the	726:728	the	726:728	The adsorbents before and after adsorption were characterized, which confirmed that adsorption process affects the morphology, composition and structure of the PPy/SCB surface.
33465610	7	55	theme	adsorption	976:985	arg1	consistent					1006:1015	consistent	1006:1015	consistent	1006:1015	The adsorption isotherm data were consistent with the Langmuir isotherm model with the adsorption capacity of 156-251 mg/g.
33465610	7	55	theme	adsorption	976:985	arg1	data					996:999	The adsorption isotherm data	972:999	The adsorption isotherm data	972:999	The adsorption isotherm data were consistent with the Langmuir isotherm model with the adsorption capacity of 156-251 mg/g.
33465610	2	56	theme	bagasse	239:245	arg1	PPy/SCB					259:265	PPy/SCB	259:265	PPy/SCB	259:265	Polypyrrole/sugarcane bagasse composites (PPy/SCB) for efficient adsorption and reduction of Cr(VI) in wastewater were prepared by in-situ polymerization.
33465610	2	56	theme	bagasse	239:245	arg1	composites					247:256	Polypyrrole/sugarcane bagasse composites	217:256	Polypyrrole/sugarcane bagasse composites (PPy/SCB) for efficient adsorption and reduction of Cr(VI) in wastewater	217:329	Polypyrrole/sugarcane bagasse composites (PPy/SCB) for efficient adsorption and reduction of Cr(VI) in wastewater were prepared by in-situ polymerization.
33465610	9	57	dep	Fourier	1498:1504	arg1	transform					1506:1514	transform	1506:1514	transform infrared spectrometry and X-ray photoelectron spectroscopy results	1506:1581	Cr(VI) anions can be chelated by oxygen-containing functional groups on SCB surface after reduction to simple cations, which was confirmed by Fourier transform infrared spectrometry and X-ray photoelectron spectroscopy results.
33465610	4	58	theme	pH	597:598	arg1	2.0					610:612	2.0	610:612	2.0	610:612	The optimal Cr(VI) adsorption capacity of PPy/SCB was achieved when the pH value was 2.0.
33465610	4	58	theme	pH	597:598	arg1	value					600:604	the pH value	593:604	the pH value	593:604	The optimal Cr(VI) adsorption capacity of PPy/SCB was achieved when the pH value was 2.0.
33465610	6	59	dep	Cr	903:904	arg1	gradient					924:931	concentration gradient	910:931	Cr(VI) concentration gradient	903:931	The adsorption kinetics data of Cr(VI) were best fitted by the pseudo-second-order model, which indicated that Cr(VI) concentration gradient played a driving role in this process.
33465610	6	59	dep	Cr	903:904	arg1	VI					906:907	VI	906:907	VI	906:907	The adsorption kinetics data of Cr(VI) were best fitted by the pseudo-second-order model, which indicated that Cr(VI) concentration gradient played a driving role in this process.
33465610	8	60	theme	electron	1215:1222	arg1	donors					1224:1229	electron donors	1215:1229	electron donors	1215:1229	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	9	61	theme	functional	1407:1416	arg1	groups					1418:1423	oxygen-containing functional groups	1389:1423	oxygen-containing functional groups on SCB surface	1389:1438	Cr(VI) anions can be chelated by oxygen-containing functional groups on SCB surface after reduction to simple cations, which was confirmed by Fourier transform infrared spectrometry and X-ray photoelectron spectroscopy results.
33465610	2	62	dep	Cr	310:311	arg1	VI					313:314	VI	313:314	VI	313:314	Polypyrrole/sugarcane bagasse composites (PPy/SCB) for efficient adsorption and reduction of Cr(VI) in wastewater were prepared by in-situ polymerization.
33465610	0	63	theme	synergistic	39:49	arg1	reduction					51:59	in-situ synergistic reduction	31:59	in-situ synergistic reduction	31:59	Enhanced removal of Cr(VI) via in-situ synergistic reduction and fixation by polypyrrole/sugarcane bagasse composites.
33465610	2	64	from	adsorption	282:291	arg1	wastewater					320:329	wastewater	320:329	wastewater	320:329	Polypyrrole/sugarcane bagasse composites (PPy/SCB) for efficient adsorption and reduction of Cr(VI) in wastewater were prepared by in-situ polymerization.
33465610	6	65	theme	driving	942:948	arg1	role					950:953	a driving role	940:953	a driving role	940:953	The adsorption kinetics data of Cr(VI) were best fitted by the pseudo-second-order model, which indicated that Cr(VI) concentration gradient played a driving role in this process.
33465610	1	66	theme	Cr	146:147	arg1	Reduction					119:127	Reduction	119:127	Reduction	119:127	Reduction and recycling of Cr(VI) can offer environmental governance and sustainable development.
33465610	1	66	theme	Cr	146:147	arg1	recycling					133:141	recycling	133:141	recycling	133:141	Reduction and recycling of Cr(VI) can offer environmental governance and sustainable development.
33465610	2	67	from	reduction	297:305	arg1	wastewater					320:329	wastewater	320:329	wastewater	320:329	Polypyrrole/sugarcane bagasse composites (PPy/SCB) for efficient adsorption and reduction of Cr(VI) in wastewater were prepared by in-situ polymerization.
33465610	6	68	theme	concentration	910:922	arg1	gradient					924:931	concentration gradient	910:931	Cr(VI) concentration gradient	903:931	The adsorption kinetics data of Cr(VI) were best fitted by the pseudo-second-order model, which indicated that Cr(VI) concentration gradient played a driving role in this process.
33465610	0	69	theme	bagasse	99:105	arg1	composites					107:116	polypyrrole/sugarcane bagasse composites	77:116	polypyrrole/sugarcane bagasse composites	77:116	Enhanced removal of Cr(VI) via in-situ synergistic reduction and fixation by polypyrrole/sugarcane bagasse composites.
33465610	4	70	dep	Cr	537:538	arg1	VI					540:541	VI	540:541	VI	540:541	The optimal Cr(VI) adsorption capacity of PPy/SCB was achieved when the pH value was 2.0.
33465610	4	70	dep	Cr	537:538	arg1	capacity					555:562	adsorption capacity	544:562	The optimal Cr(VI) adsorption capacity of PPy/SCB	525:573	The optimal Cr(VI) adsorption capacity of PPy/SCB was achieved when the pH value was 2.0.
33465610	9	71	theme	simple	1459:1464	arg1	cations					1466:1472	simple cations	1459:1472	simple cations	1459:1472	Cr(VI) anions can be chelated by oxygen-containing functional groups on SCB surface after reduction to simple cations, which was confirmed by Fourier transform infrared spectrometry and X-ray photoelectron spectroscopy results.
33465610	3	72	from	effects	376:382	arg1	capacity					501:508	adsorption capacity	490:508	adsorption capacity	490:508	The effects of the initial pH, Cr concentration, adsorbent dosage, contact time, composition ratio and temperature on adsorption capacity were explored.
33465610	4	73	theme	optimal	529:535	arg1	Cr					537:538	The optimal Cr(VI) adsorption capacity of PPy/SCB	525:573	The optimal Cr(VI) adsorption capacity of PPy/SCB	525:573	The optimal Cr(VI) adsorption capacity of PPy/SCB was achieved when the pH value was 2.0.
33465610	8	74	theme	rapid	1286:1290	arg1	removal					1292:1298	the rapid removal	1282:1298	the rapid removal	1282:1298	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	3	75	theme	Cr	403:404	arg1	concentration					406:418	Cr concentration	403:418	Cr concentration	403:418	The effects of the initial pH, Cr concentration, adsorbent dosage, contact time, composition ratio and temperature on adsorption capacity were explored.
33465610	0	76	theme	Cr	20:21	arg1	removal					9:15	removal	9:15	removal of Cr(VI)	9:25	Enhanced removal of Cr(VI) via in-situ synergistic reduction and fixation by polypyrrole/sugarcane bagasse composites.
33465610	3	77	theme	contact	439:445	arg1	time					447:450	contact time	439:450	contact time	439:450	The effects of the initial pH, Cr concentration, adsorbent dosage, contact time, composition ratio and temperature on adsorption capacity were explored.
33465610	6	78	theme	Cr	824:825	arg1	data					816:819	The adsorption kinetics data	792:819	The adsorption kinetics data of Cr(VI)	792:829	The adsorption kinetics data of Cr(VI) were best fitted by the pseudo-second-order model, which indicated that Cr(VI) concentration gradient played a driving role in this process.
33465610	3	79	theme	pH	399:400	arg1	effects					376:382	The effects	372:382	The effects of the initial pH, Cr concentration, adsorbent dosage, contact time, composition ratio and temperature on adsorption capacity	372:508	The effects of the initial pH, Cr concentration, adsorbent dosage, contact time, composition ratio and temperature on adsorption capacity were explored.
33465610	0	80	theme	polypyrrole/sugarcane	77:97	arg1	composites					107:116	polypyrrole/sugarcane bagasse composites	77:116	polypyrrole/sugarcane bagasse composites	77:116	Enhanced removal of Cr(VI) via in-situ synergistic reduction and fixation by polypyrrole/sugarcane bagasse composites.
33465610	7	81	theme	adsorption	1059:1068	arg1	capacity					1070:1077	the adsorption capacity	1055:1077	the adsorption capacity of 156-251 mg/g	1055:1093	The adsorption isotherm data were consistent with the Langmuir isotherm model with the adsorption capacity of 156-251 mg/g.
33465610	7	82	with	consistent	1006:1015	arg1	model					1044:1048	the Langmuir isotherm model	1022:1048	the Langmuir isotherm model with the adsorption capacity of 156-251 mg/g	1022:1093	The adsorption isotherm data were consistent with the Langmuir isotherm model with the adsorption capacity of 156-251 mg/g.
33465610	8	83	theme	total	1326:1330	arg1	Cr					1332:1333	total Cr	1326:1333	total Cr	1326:1333	Synergic effect of PPy and SCB during the processes of electrostatic adsorption and ion exchange, in-situ reduction of electron donors, and coordination were found to be responsible for the rapid removal and toxicity reduction of total Cr in aqueous solution.
33465610	3	84	theme	ratio	465:469	arg1	effects					376:382	The effects	372:382	The effects of the initial pH, Cr concentration, adsorbent dosage, contact time, composition ratio and temperature on adsorption capacity	372:508	The effects of the initial pH, Cr concentration, adsorbent dosage, contact time, composition ratio and temperature on adsorption capacity were explored.
33465610	6	85	theme	adsorption	796:805	arg1	data					816:819	The adsorption kinetics data	792:819	The adsorption kinetics data of Cr(VI)	792:829	The adsorption kinetics data of Cr(VI) were best fitted by the pseudo-second-order model, which indicated that Cr(VI) concentration gradient played a driving role in this process.
33465610	0	86	dep	Cr	20:21	arg1	VI					23:24	VI	23:24	VI	23:24	Enhanced removal of Cr(VI) via in-situ synergistic reduction and fixation by polypyrrole/sugarcane bagasse composites.
33465610	2	87	theme	Polypyrrole/sugarcane	217:237	arg1	PPy/SCB					259:265	PPy/SCB	259:265	PPy/SCB	259:265	Polypyrrole/sugarcane bagasse composites (PPy/SCB) for efficient adsorption and reduction of Cr(VI) in wastewater were prepared by in-situ polymerization.
33465610	2	87	theme	Polypyrrole/sugarcane	217:237	arg1	composites					247:256	Polypyrrole/sugarcane bagasse composites	217:256	Polypyrrole/sugarcane bagasse composites (PPy/SCB) for efficient adsorption and reduction of Cr(VI) in wastewater	217:329	Polypyrrole/sugarcane bagasse composites (PPy/SCB) for efficient adsorption and reduction of Cr(VI) in wastewater were prepared by in-situ polymerization.
33465610	4	88	theme	PPy/SCB	567:573	arg1	Cr					537:538	The optimal Cr(VI) adsorption capacity of PPy/SCB	525:573	The optimal Cr(VI) adsorption capacity of PPy/SCB	525:573	The optimal Cr(VI) adsorption capacity of PPy/SCB was achieved when the pH value was 2.0.
33465610	3	89	theme	adsorption	490:499	arg1	capacity					501:508	adsorption capacity	490:508	adsorption capacity	490:508	The effects of the initial pH, Cr concentration, adsorbent dosage, contact time, composition ratio and temperature on adsorption capacity were explored.
33465610	9	90	theme	X-ray	1542:1546	arg1	spectroscopy					1562:1573	X-ray photoelectron spectroscopy	1542:1573	X-ray photoelectron spectroscopy	1542:1573	Cr(VI) anions can be chelated by oxygen-containing functional groups on SCB surface after reduction to simple cations, which was confirmed by Fourier transform infrared spectrometry and X-ray photoelectron spectroscopy results.
33465610	10	91	theme	waste	1648:1652	arg1	biomass					1654:1660	waste biomass	1648:1660	waste biomass	1648:1660	This study provides a reasonable scheme for the targeted use of waste biomass for heavy metal pollution control.
34750447	7	0	with	materials	1341:1349	arg1	capacity					1375:1382	high antibacterial capacity	1356:1382	high antibacterial capacity	1356:1382	As such these hydrogel composites show great promise as wound dressing materials with high antibacterial capacity.
34750447	5	1	dep	in	819:820	arg1	vivo					822:825	vivo	822:825	vivo	822:825	We then show that the hydrogel-nanoparticle composite promotes wound healing in in vivo studies on rats, accelerating wound closure by > 40% and reducing bacterial counts by 60% compared to commercial antibacterial gels.
34750447	1	2	theme	enormous	176:183	arg1	interest					185:192	enormous interest	176:192	enormous interest	176:192	Biocompatible materials that act as scaffolds for regenerative medicine are of enormous interest.
34750447	4	3	from	production	679:688	arg1	studies					702:708	in vitro studies	693:708	in vitro studies of human dermal fibroblasts	693:736	We show that the curcumin-bound silver nanoparticles themselves exhibit low cytotoxicity and enhance proliferation, migration, and collagen production in in vitro studies of human dermal fibroblasts.
34750447	6	4	theme	hydrogel	994:1001	arg1	composite					1003:1011	the hydrogel composite	990:1011	the hydrogel composite	990:1011	Histopathology indicates that the hydrogel composite enhances transition from the inflammation to proliferation stage of healing, promoting the formation of fibroblasts and new blood vessels, while target gene expression studies confirm that the accelerated tissue remodeling occurs along the normal pathways.
34750447	5	5	theme	by > 40	871:877	arg1	%					878:878	%	878:878	%	878:878	We then show that the hydrogel-nanoparticle composite promotes wound healing in in vivo studies on rats, accelerating wound closure by > 40% and reducing bacterial counts by 60% compared to commercial antibacterial gels.
34750447	5	6	from	healing	808:814	arg1	rats					838:841	rats	838:841	rats	838:841	We then show that the hydrogel-nanoparticle composite promotes wound healing in in vivo studies on rats, accelerating wound closure by > 40% and reducing bacterial counts by 60% compared to commercial antibacterial gels.
34750447	5	6	from	healing	808:814	arg1	studies					827:833	in vivo studies	819:833	in vivo studies on rats	819:841	We then show that the hydrogel-nanoparticle composite promotes wound healing in in vivo studies on rats, accelerating wound closure by > 40% and reducing bacterial counts by 60% compared to commercial antibacterial gels.
34750447	4	7	theme	silver	571:576	arg1	nanoparticles					578:590	the curcumin-bound silver nanoparticles	552:590	the curcumin-bound silver nanoparticles themselves	552:601	We show that the curcumin-bound silver nanoparticles themselves exhibit low cytotoxicity and enhance proliferation, migration, and collagen production in in vitro studies of human dermal fibroblasts.
34750447	3	8	theme	hydrogel	433:440	arg1	material					469:476	an injectable material	455:476	an injectable material with exceptional wound healing and antibacterial properties	455:536	Here we demonstrate that a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite is an injectable material with exceptional wound healing and antibacterial properties.
34750447	3	8	theme	hydrogel	433:440	arg1	composite					442:450	a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite	382:450	a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite	382:450	Here we demonstrate that a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite is an injectable material with exceptional wound healing and antibacterial properties.
34750447	6	9	theme	blood	1137:1141	arg1	vessels					1143:1149	new blood vessels	1133:1149	new blood vessels	1133:1149	Histopathology indicates that the hydrogel composite enhances transition from the inflammation to proliferation stage of healing, promoting the formation of fibroblasts and new blood vessels, while target gene expression studies confirm that the accelerated tissue remodeling occurs along the normal pathways.
34750447	4	10	theme	collagen	670:677	arg1	production					679:688	collagen production	670:688	collagen production	670:688	We show that the curcumin-bound silver nanoparticles themselves exhibit low cytotoxicity and enhance proliferation, migration, and collagen production in in vitro studies of human dermal fibroblasts.
34750447	3	11	theme	guar	384:387	arg1	material					469:476	an injectable material	455:476	an injectable material with exceptional wound healing and antibacterial properties	455:536	Here we demonstrate that a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite is an injectable material with exceptional wound healing and antibacterial properties.
34750447	3	11	theme	guar	384:387	arg1	composite					442:450	a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite	382:450	a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite	382:450	Here we demonstrate that a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite is an injectable material with exceptional wound healing and antibacterial properties.
34750447	6	12	theme	normal	1253:1258	arg1	pathways					1260:1267	the normal pathways	1249:1267	the normal pathways	1249:1267	Histopathology indicates that the hydrogel composite enhances transition from the inflammation to proliferation stage of healing, promoting the formation of fibroblasts and new blood vessels, while target gene expression studies confirm that the accelerated tissue remodeling occurs along the normal pathways.
34750447	6	13	theme	healing	1081:1087	arg1	stage					1072:1076	proliferation stage	1058:1076	proliferation stage of healing	1058:1087	Histopathology indicates that the hydrogel composite enhances transition from the inflammation to proliferation stage of healing, promoting the formation of fibroblasts and new blood vessels, while target gene expression studies confirm that the accelerated tissue remodeling occurs along the normal pathways.
34750447	7	14	theme	hydrogel	1284:1291	arg1	composites					1293:1302	these hydrogel composites	1278:1302	these hydrogel composites	1278:1302	As such these hydrogel composites show great promise as wound dressing materials with high antibacterial capacity.
34750447	6	15	theme	expression	1170:1179	arg1	studies					1181:1187	target gene expression studies	1158:1187	target gene expression studies	1158:1187	Histopathology indicates that the hydrogel composite enhances transition from the inflammation to proliferation stage of healing, promoting the formation of fibroblasts and new blood vessels, while target gene expression studies confirm that the accelerated tissue remodeling occurs along the normal pathways.
34750447	2	16	theme	safety	312:317	arg1	scarce					349:354	scarce	349:354	scarce	349:354	Hydrogel-nanoparticle composites have great potential in this regard, however evaluations of their wound healing and safety in vivo in animal studies are scarce.
34750447	2	16	theme	safety	312:317	arg1	evaluations					273:283	evaluations	273:283	evaluations of their wound healing and safety in vivo in animal studies	273:343	Hydrogel-nanoparticle composites have great potential in this regard, however evaluations of their wound healing and safety in vivo in animal studies are scarce.
34750447	6	17	theme	target	1158:1163	arg1	studies					1181:1187	target gene expression studies	1158:1187	target gene expression studies	1158:1187	Histopathology indicates that the hydrogel composite enhances transition from the inflammation to proliferation stage of healing, promoting the formation of fibroblasts and new blood vessels, while target gene expression studies confirm that the accelerated tissue remodeling occurs along the normal pathways.
34750447	3	18	theme	exceptional	483:493	arg1	healing					501:507	exceptional wound healing	483:507	exceptional wound healing	483:507	Here we demonstrate that a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite is an injectable material with exceptional wound healing and antibacterial properties.
34750447	4	19	from	migration	655:663	arg1	studies					702:708	in vitro studies	693:708	in vitro studies of human dermal fibroblasts	693:736	We show that the curcumin-bound silver nanoparticles themselves exhibit low cytotoxicity and enhance proliferation, migration, and collagen production in in vitro studies of human dermal fibroblasts.
34750447	5	20	theme	wound	857:861	arg1	closure					863:869	wound closure	857:869	wound closure by > 40%	857:878	We then show that the hydrogel-nanoparticle composite promotes wound healing in in vivo studies on rats, accelerating wound closure by > 40% and reducing bacterial counts by 60% compared to commercial antibacterial gels.
34750447	1	21	theme	Biocompatible	97:109	arg1	scaffolds					133:141	scaffolds	133:141	scaffolds for regenerative medicine	133:167	Biocompatible materials that act as scaffolds for regenerative medicine are of enormous interest.
34750447	1	21	theme	Biocompatible	97:109	arg1	materials					111:119	Biocompatible materials	97:119	Biocompatible materials that act as scaffolds for regenerative medicine	97:167	Biocompatible materials that act as scaffolds for regenerative medicine are of enormous interest.
34750447	2	22	from	evaluations	273:283	arg1	studies					337:343	animal studies	330:343	animal studies	330:343	Hydrogel-nanoparticle composites have great potential in this regard, however evaluations of their wound healing and safety in vivo in animal studies are scarce.
34750447	3	23	theme	wound	495:499	arg1	healing					501:507	exceptional wound healing	483:507	exceptional wound healing	483:507	Here we demonstrate that a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite is an injectable material with exceptional wound healing and antibacterial properties.
34750447	0	24	theme	wound	9:13	arg1	healing					15:21	wound healing	9:21	Enhanced wound healing properties of guar	0:40	Enhanced wound healing properties of guar gum/curcumin-stabilized silver nanoparticle hydrogels.
34750447	5	25	dep	promotes	793:800	arg1	reducing					884:891	reducing	884:891	reducing bacterial counts by 60% compared to commercial antibacterial gels	884:957	We then show that the hydrogel-nanoparticle composite promotes wound healing in in vivo studies on rats, accelerating wound closure by > 40% and reducing bacterial counts by 60% compared to commercial antibacterial gels.
34750447	5	25	dep	promotes	793:800	arg1	accelerating					844:855	accelerating	844:855	accelerating wound closure by > 40%	844:878	We then show that the hydrogel-nanoparticle composite promotes wound healing in in vivo studies on rats, accelerating wound closure by > 40% and reducing bacterial counts by 60% compared to commercial antibacterial gels.
34750447	7	26	theme	antibacterial	1361:1373	arg1	capacity					1375:1382	high antibacterial capacity	1356:1382	high antibacterial capacity	1356:1382	As such these hydrogel composites show great promise as wound dressing materials with high antibacterial capacity.
34750447	5	27	theme	antibacterial	940:952	arg1	gels					954:957	commercial antibacterial gels	929:957	commercial antibacterial gels	929:957	We then show that the hydrogel-nanoparticle composite promotes wound healing in in vivo studies on rats, accelerating wound closure by > 40% and reducing bacterial counts by 60% compared to commercial antibacterial gels.
34750447	0	28	theme	Enhanced	0:7	arg1	properties					23:32	Enhanced wound healing properties	0:32	Enhanced wound healing properties of guar	0:40	Enhanced wound healing properties of guar gum/curcumin-stabilized silver nanoparticle hydrogels.
34750447	5	29	from	rats	838:841	arg1	healing					808:814	wound healing	802:814	wound healing in in vivo studies on rats	802:841	We then show that the hydrogel-nanoparticle composite promotes wound healing in in vivo studies on rats, accelerating wound closure by > 40% and reducing bacterial counts by 60% compared to commercial antibacterial gels.
34750447	5	30	theme	commercial	929:938	arg1	gels					954:957	commercial antibacterial gels	929:957	commercial antibacterial gels	929:957	We then show that the hydrogel-nanoparticle composite promotes wound healing in in vivo studies on rats, accelerating wound closure by > 40% and reducing bacterial counts by 60% compared to commercial antibacterial gels.
34750447	2	31	contain	have	228:231	arg1	composites					217:226	Hydrogel-nanoparticle composites	195:226	Hydrogel-nanoparticle composites	195:226	Hydrogel-nanoparticle composites have great potential in this regard, however evaluations of their wound healing and safety in vivo in animal studies are scarce.
34750447	2	31	contain	have	228:231	arg2	potential					239:247	great potential	233:247	great potential	233:247	Hydrogel-nanoparticle composites have great potential in this regard, however evaluations of their wound healing and safety in vivo in animal studies are scarce.
34750447	3	32	theme	gum/curcumin-stabilized	389:411	arg1	material					469:476	an injectable material	455:476	an injectable material with exceptional wound healing and antibacterial properties	455:536	Here we demonstrate that a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite is an injectable material with exceptional wound healing and antibacterial properties.
34750447	3	32	theme	gum/curcumin-stabilized	389:411	arg1	composite					442:450	a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite	382:450	a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite	382:450	Here we demonstrate that a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite is an injectable material with exceptional wound healing and antibacterial properties.
34750447	4	33	from	proliferation	640:652	arg1	studies					702:708	in vitro studies	693:708	in vitro studies of human dermal fibroblasts	693:736	We show that the curcumin-bound silver nanoparticles themselves exhibit low cytotoxicity and enhance proliferation, migration, and collagen production in in vitro studies of human dermal fibroblasts.
34750447	2	34	theme	animal	330:335	arg1	studies					337:343	animal studies	330:343	animal studies	330:343	Hydrogel-nanoparticle composites have great potential in this regard, however evaluations of their wound healing and safety in vivo in animal studies are scarce.
34750447	6	35	theme	gene	1165:1168	arg1	studies					1181:1187	target gene expression studies	1158:1187	target gene expression studies	1158:1187	Histopathology indicates that the hydrogel composite enhances transition from the inflammation to proliferation stage of healing, promoting the formation of fibroblasts and new blood vessels, while target gene expression studies confirm that the accelerated tissue remodeling occurs along the normal pathways.
34750447	3	36	theme	antibacterial	513:525	arg1	properties					527:536	antibacterial properties	513:536	antibacterial properties	513:536	Here we demonstrate that a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite is an injectable material with exceptional wound healing and antibacterial properties.
34750447	3	37	theme	silver	413:418	arg1	material					469:476	an injectable material	455:476	an injectable material with exceptional wound healing and antibacterial properties	455:536	Here we demonstrate that a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite is an injectable material with exceptional wound healing and antibacterial properties.
34750447	3	37	theme	silver	413:418	arg1	composite					442:450	a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite	382:450	a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite	382:450	Here we demonstrate that a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite is an injectable material with exceptional wound healing and antibacterial properties.
34750447	6	38	theme	proliferation	1058:1070	arg1	stage					1072:1076	proliferation stage	1058:1076	proliferation stage of healing	1058:1087	Histopathology indicates that the hydrogel composite enhances transition from the inflammation to proliferation stage of healing, promoting the formation of fibroblasts and new blood vessels, while target gene expression studies confirm that the accelerated tissue remodeling occurs along the normal pathways.
34750447	6	39	theme	vessels	1143:1149	arg1	formation					1104:1112	the formation	1100:1112	the formation of fibroblasts and new blood vessels	1100:1149	Histopathology indicates that the hydrogel composite enhances transition from the inflammation to proliferation stage of healing, promoting the formation of fibroblasts and new blood vessels, while target gene expression studies confirm that the accelerated tissue remodeling occurs along the normal pathways.
34750447	0	40	theme	guar	37:40	arg1	properties					23:32	Enhanced wound healing properties	0:32	Enhanced wound healing properties of guar	0:40	Enhanced wound healing properties of guar gum/curcumin-stabilized silver nanoparticle hydrogels.
34750447	5	41	theme	wound	802:806	arg1	healing					808:814	wound healing	802:814	wound healing in in vivo studies on rats	802:841	We then show that the hydrogel-nanoparticle composite promotes wound healing in in vivo studies on rats, accelerating wound closure by > 40% and reducing bacterial counts by 60% compared to commercial antibacterial gels.
34750447	5	42	from	studies	827:833	arg1	rats					838:841	rats	838:841	rats	838:841	We then show that the hydrogel-nanoparticle composite promotes wound healing in in vivo studies on rats, accelerating wound closure by > 40% and reducing bacterial counts by 60% compared to commercial antibacterial gels.
34750447	4	43	theme	dermal	719:724	arg1	fibroblasts					726:736	human dermal fibroblasts	713:736	human dermal fibroblasts	713:736	We show that the curcumin-bound silver nanoparticles themselves exhibit low cytotoxicity and enhance proliferation, migration, and collagen production in in vitro studies of human dermal fibroblasts.
34750447	3	44	theme	injectable	458:467	arg1	material					469:476	an injectable material	455:476	an injectable material with exceptional wound healing and antibacterial properties	455:536	Here we demonstrate that a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite is an injectable material with exceptional wound healing and antibacterial properties.
34750447	3	44	theme	injectable	458:467	arg1	composite					442:450	a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite	382:450	a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite	382:450	Here we demonstrate that a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite is an injectable material with exceptional wound healing and antibacterial properties.
34750447	2	45	theme	Hydrogel-nanoparticle	195:215	arg1	composites					217:226	Hydrogel-nanoparticle composites	195:226	Hydrogel-nanoparticle composites	195:226	Hydrogel-nanoparticle composites have great potential in this regard, however evaluations of their wound healing and safety in vivo in animal studies are scarce.
34750447	5	46	theme	bacterial	893:901	arg1	counts					903:908	bacterial counts	893:908	bacterial counts	893:908	We then show that the hydrogel-nanoparticle composite promotes wound healing in in vivo studies on rats, accelerating wound closure by > 40% and reducing bacterial counts by 60% compared to commercial antibacterial gels.
34750447	4	47	theme	human	713:717	arg1	fibroblasts					726:736	human dermal fibroblasts	713:736	human dermal fibroblasts	713:736	We show that the curcumin-bound silver nanoparticles themselves exhibit low cytotoxicity and enhance proliferation, migration, and collagen production in in vitro studies of human dermal fibroblasts.
34750447	0	48	theme	silver	66:71	arg1	hydrogels					86:94	silver nanoparticle hydrogels	66:94	silver nanoparticle hydrogels	66:94	Enhanced wound healing properties of guar gum/curcumin-stabilized silver nanoparticle hydrogels.
34750447	3	49	with	material	469:476	arg1	healing					501:507	exceptional wound healing	483:507	exceptional wound healing	483:507	Here we demonstrate that a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite is an injectable material with exceptional wound healing and antibacterial properties.
34750447	3	49	with	material	469:476	arg1	properties					527:536	antibacterial properties	513:536	antibacterial properties	513:536	Here we demonstrate that a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite is an injectable material with exceptional wound healing and antibacterial properties.
34750447	7	50	theme	dressing	1332:1339	arg1	materials					1341:1349	wound dressing materials	1326:1349	wound dressing materials with high antibacterial capacity	1326:1382	As such these hydrogel composites show great promise as wound dressing materials with high antibacterial capacity.
34750447	7	50	theme	dressing	1332:1339	arg1	promise					1315:1321	great promise	1309:1321	great promise	1309:1321	As such these hydrogel composites show great promise as wound dressing materials with high antibacterial capacity.
34750447	5	51	theme	%	878:878	arg1	closure					863:869	wound closure	857:869	wound closure by > 40%	857:878	We then show that the hydrogel-nanoparticle composite promotes wound healing in in vivo studies on rats, accelerating wound closure by > 40% and reducing bacterial counts by 60% compared to commercial antibacterial gels.
34750447	6	52	theme	new	1133:1135	arg1	vessels					1143:1149	new blood vessels	1133:1149	new blood vessels	1133:1149	Histopathology indicates that the hydrogel composite enhances transition from the inflammation to proliferation stage of healing, promoting the formation of fibroblasts and new blood vessels, while target gene expression studies confirm that the accelerated tissue remodeling occurs along the normal pathways.
34750447	7	53	theme	great	1309:1313	arg1	materials					1341:1349	wound dressing materials	1326:1349	wound dressing materials with high antibacterial capacity	1326:1382	As such these hydrogel composites show great promise as wound dressing materials with high antibacterial capacity.
34750447	7	53	theme	great	1309:1313	arg1	promise					1315:1321	great promise	1309:1321	great promise	1309:1321	As such these hydrogel composites show great promise as wound dressing materials with high antibacterial capacity.
34750447	4	54	theme	low	611:613	arg1	cytotoxicity					615:626	low cytotoxicity	611:626	low cytotoxicity	611:626	We show that the curcumin-bound silver nanoparticles themselves exhibit low cytotoxicity and enhance proliferation, migration, and collagen production in in vitro studies of human dermal fibroblasts.
34750447	6	55	from	inflammation	1042:1053	arg1	transition					1022:1031	transition	1022:1031	transition from the inflammation to proliferation stage of healing	1022:1087	Histopathology indicates that the hydrogel composite enhances transition from the inflammation to proliferation stage of healing, promoting the formation of fibroblasts and new blood vessels, while target gene expression studies confirm that the accelerated tissue remodeling occurs along the normal pathways.
34750447	4	56	dep	in	693:694	arg1	vitro					696:700	vitro	696:700	vitro	696:700	We show that the curcumin-bound silver nanoparticles themselves exhibit low cytotoxicity and enhance proliferation, migration, and collagen production in in vitro studies of human dermal fibroblasts.
34750447	2	57	theme	healing	300:306	arg1	scarce					349:354	scarce	349:354	scarce	349:354	Hydrogel-nanoparticle composites have great potential in this regard, however evaluations of their wound healing and safety in vivo in animal studies are scarce.
34750447	2	57	theme	healing	300:306	arg1	evaluations					273:283	evaluations	273:283	evaluations of their wound healing and safety in vivo in animal studies	273:343	Hydrogel-nanoparticle composites have great potential in this regard, however evaluations of their wound healing and safety in vivo in animal studies are scarce.
34750447	6	58	theme	fibroblasts	1117:1127	arg1	formation					1104:1112	the formation	1100:1112	the formation of fibroblasts and new blood vessels	1100:1149	Histopathology indicates that the hydrogel composite enhances transition from the inflammation to proliferation stage of healing, promoting the formation of fibroblasts and new blood vessels, while target gene expression studies confirm that the accelerated tissue remodeling occurs along the normal pathways.
34750447	1	59	theme	regenerative	147:158	arg1	medicine					160:167	regenerative medicine	147:167	regenerative medicine	147:167	Biocompatible materials that act as scaffolds for regenerative medicine are of enormous interest.
34750447	6	60	theme	accelerated	1206:1216	arg1	remodeling					1225:1234	the accelerated tissue remodeling	1202:1234	the accelerated tissue remodeling	1202:1234	Histopathology indicates that the hydrogel composite enhances transition from the inflammation to proliferation stage of healing, promoting the formation of fibroblasts and new blood vessels, while target gene expression studies confirm that the accelerated tissue remodeling occurs along the normal pathways.
34750447	5	61	theme	hydrogel-nanoparticle	761:781	arg1	composite					783:791	the hydrogel-nanoparticle composite	757:791	the hydrogel-nanoparticle composite	757:791	We then show that the hydrogel-nanoparticle composite promotes wound healing in in vivo studies on rats, accelerating wound closure by > 40% and reducing bacterial counts by 60% compared to commercial antibacterial gels.
34750447	2	62	theme	wound	294:298	arg1	healing					300:306	their wound healing	288:306	their wound healing	288:306	Hydrogel-nanoparticle composites have great potential in this regard, however evaluations of their wound healing and safety in vivo in animal studies are scarce.
34750447	5	63	theme	in	819:820	arg1	studies					827:833	in vivo studies	819:833	in vivo studies on rats	819:841	We then show that the hydrogel-nanoparticle composite promotes wound healing in in vivo studies on rats, accelerating wound closure by > 40% and reducing bacterial counts by 60% compared to commercial antibacterial gels.
34750447	6	64	theme	tissue	1218:1223	arg1	remodeling					1225:1234	the accelerated tissue remodeling	1202:1234	the accelerated tissue remodeling	1202:1234	Histopathology indicates that the hydrogel composite enhances transition from the inflammation to proliferation stage of healing, promoting the formation of fibroblasts and new blood vessels, while target gene expression studies confirm that the accelerated tissue remodeling occurs along the normal pathways.
34750447	7	65	theme	wound	1326:1330	arg1	materials					1341:1349	wound dressing materials	1326:1349	wound dressing materials with high antibacterial capacity	1326:1382	As such these hydrogel composites show great promise as wound dressing materials with high antibacterial capacity.
34750447	7	65	theme	wound	1326:1330	arg1	promise					1315:1321	great promise	1309:1321	great promise	1309:1321	As such these hydrogel composites show great promise as wound dressing materials with high antibacterial capacity.
34750447	2	66	theme	great	233:237	arg1	potential					239:247	great potential	233:247	great potential	233:247	Hydrogel-nanoparticle composites have great potential in this regard, however evaluations of their wound healing and safety in vivo in animal studies are scarce.
34750447	7	67	theme	high	1356:1359	arg1	capacity					1375:1382	high antibacterial capacity	1356:1382	high antibacterial capacity	1356:1382	As such these hydrogel composites show great promise as wound dressing materials with high antibacterial capacity.
34750447	4	68	theme	fibroblasts	726:736	arg1	studies					702:708	in vitro studies	693:708	in vitro studies of human dermal fibroblasts	693:736	We show that the curcumin-bound silver nanoparticles themselves exhibit low cytotoxicity and enhance proliferation, migration, and collagen production in in vitro studies of human dermal fibroblasts.
34750447	0	69	theme	nanoparticle	73:84	arg1	hydrogels					86:94	silver nanoparticle hydrogels	66:94	silver nanoparticle hydrogels	66:94	Enhanced wound healing properties of guar gum/curcumin-stabilized silver nanoparticle hydrogels.
34750447	4	70	theme	curcumin-bound	556:569	arg1	nanoparticles					578:590	the curcumin-bound silver nanoparticles	552:590	the curcumin-bound silver nanoparticles themselves	552:601	We show that the curcumin-bound silver nanoparticles themselves exhibit low cytotoxicity and enhance proliferation, migration, and collagen production in in vitro studies of human dermal fibroblasts.
34750447	0	71	theme	healing	15:21	arg1	properties					23:32	Enhanced wound healing properties	0:32	Enhanced wound healing properties of guar	0:40	Enhanced wound healing properties of guar gum/curcumin-stabilized silver nanoparticle hydrogels.
34750447	3	72	theme	nanoparticle	420:431	arg1	material					469:476	an injectable material	455:476	an injectable material with exceptional wound healing and antibacterial properties	455:536	Here we demonstrate that a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite is an injectable material with exceptional wound healing and antibacterial properties.
34750447	3	72	theme	nanoparticle	420:431	arg1	composite					442:450	a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite	382:450	a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite	382:450	Here we demonstrate that a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite is an injectable material with exceptional wound healing and antibacterial properties.
34750447	4	73	theme	in	693:694	arg1	studies					702:708	in vitro studies	693:708	in vitro studies of human dermal fibroblasts	693:736	We show that the curcumin-bound silver nanoparticles themselves exhibit low cytotoxicity and enhance proliferation, migration, and collagen production in in vitro studies of human dermal fibroblasts.
34175512	1	0	theme	energy	264:269	arg1	consumption					240:250	the consumption	236:250	the consumption of aeration energy	236:269	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	1	0	theme	energy	264:269	arg1	production					211:220	the mass production	202:220	the mass production of sludge	202:230	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	1	0	theme	energy	264:269	arg1	loss					179:182	the easy loss	170:182	the easy loss of microorganism	170:199	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	6	1	theme	microbial	1170:1178	arg1	action					1180:1185	microbial action	1170:1185	microbial action	1170:1185	Both adsorption and microbial action contributed to ammonia nitrogen removal, and their contributions were 54.3% and 42.2%, respectively.
34175512	9	2	theme	ammonia	1868:1874	arg1	removal					1885:1891	ammonia nitrogen removal	1868:1891	ammonia nitrogen removal from wastewater	1868:1907	To sum up, the immobilized bacteria pellets using chitosan-sodium alginate as an embedding agent have a promising prospect in ammonia nitrogen removal from wastewater.
34175512	8	3	theme	chitosan	1609:1616	arg1	groups					1631:1636	carboxyl groups	1622:1636	carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life	1622:1739	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	8	3	theme	chitosan	1609:1616	arg1	groups					1599:1604	amino groups	1593:1604	amino groups of chitosan	1593:1616	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	8	3	theme	chitosan	1609:1616	arg1	alginate					1648:1655	sodium alginate	1641:1655	sodium alginate	1641:1655	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	8	3	theme	chitosan	1609:1616	arg1	chitosan					1609:1616	chitosan	1609:1616	chitosan	1609:1616	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	1	4	theme	biological	278:287	arg1	treatment					289:297	biological treatment	278:297	biological treatment of anaerobically digested swine wastewater	278:340	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	8	5	theme	bacteria	1691:1698	arg1	pellets					1700:1706	the immobilized bacteria pellets	1675:1706	the immobilized bacteria pellets	1675:1706	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	1	6	theme	microorganism	187:199	arg1	consumption					240:250	the consumption	236:250	the consumption of aeration energy	236:269	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	1	6	theme	microorganism	187:199	arg1	production					211:220	the mass production	202:220	the mass production of sludge	202:230	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	1	6	theme	microorganism	187:199	arg1	loss					179:182	the easy loss	170:182	the easy loss of microorganism	170:199	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	7	7	theme	mono	1392:1395	arg1	alginate					1397:1404	mono alginate	1392:1404	mono alginate	1392:1404	Compared with the immobilized bacteria pellets using chitosan-sodium alginate as carrier, the one using mono alginate as carrier had a weaker ability to remove ammonia nitrogen, with a removal efficiency of 67.4%.
34175512	0	8	theme	swine	140:144	arg1	wastewater					146:155	anaerobically digested swine wastewater	117:155	anaerobically digested swine wastewater	117:155	Effective immobilization of Bacillus subtilis in chitosan-sodium alginate composite carrier for ammonia removal from anaerobically digested swine wastewater.
34175512	1	9	theme	immobilized	413:423	arg1	pellets					434:440	immobilized bacteria pellets	413:440	immobilized bacteria pellets	413:440	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	3	10	theme	bacteria	628:635	arg1	pellets					637:643	immobilized bacteria pellets	616:643	immobilized bacteria pellets	616:643	The performance of immobilized bacteria pellets to remove ammonia nitrogen from ADSW was determined and the corresponding mechanism was investigated.
34175512	1	11	theme	sludge	225:230	arg1	consumption					240:250	the consumption	236:250	the consumption of aeration energy	236:269	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	1	11	theme	sludge	225:230	arg1	production					211:220	the mass production	202:220	the mass production of sludge	202:230	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	1	11	theme	sludge	225:230	arg1	loss					179:182	the easy loss	170:182	the easy loss of microorganism	170:199	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	5	12	theme	ADSW	1047:1050	arg1	treatment					1052:1060	ADSW treatment	1047:1060	ADSW treatment	1047:1060	In ADSW treatment, at pH 6, 20 g/L of the immobilized bacteria pellets removed 96.5% of ammonia nitrogen.
34175512	3	13	theme	ammonia	655:661	arg1	nitrogen					663:670	ammonia nitrogen	655:670	ammonia nitrogen	655:670	The performance of immobilized bacteria pellets to remove ammonia nitrogen from ADSW was determined and the corresponding mechanism was investigated.
34175512	6	14	theme	ammonia	1202:1208	arg1	removal					1219:1225	ammonia nitrogen removal	1202:1225	ammonia nitrogen removal	1202:1225	Both adsorption and microbial action contributed to ammonia nitrogen removal, and their contributions were 54.3% and 42.2%, respectively.
34175512	7	15	theme	chitosan-sodium	1341:1355	arg1	alginate					1357:1364	chitosan-sodium alginate	1341:1364	chitosan-sodium alginate	1341:1364	Compared with the immobilized bacteria pellets using chitosan-sodium alginate as carrier, the one using mono alginate as carrier had a weaker ability to remove ammonia nitrogen, with a removal efficiency of 67.4%.
34175512	0	16	theme	composite	74:82	arg1	carrier					84:90	chitosan-sodium alginate composite carrier	49:90	chitosan-sodium alginate composite carrier for ammonia removal from anaerobically digested swine wastewater	49:155	Effective immobilization of Bacillus subtilis in chitosan-sodium alginate composite carrier for ammonia removal from anaerobically digested swine wastewater.
34175512	5	17	theme	pellets	1107:1113	arg1	20 g/L					1072:1077	20 g/L	1072:1077	20 g/L of the immobilized bacteria pellets	1072:1113	In ADSW treatment, at pH 6, 20 g/L of the immobilized bacteria pellets removed 96.5% of ammonia nitrogen.
34175512	1	18	theme	chitosan-sodium	359:373	arg1	carrier					394:400	chitosan-sodium alginate composite carrier	359:400	chitosan-sodium alginate composite carrier	359:400	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	4	19	theme	embedding	984:992	arg1	time					994:997	embedding time	984:997	embedding time of 32 min	984:1007	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	1	20	theme	composite	384:392	arg1	carrier					394:400	chitosan-sodium alginate composite carrier	359:400	chitosan-sodium alginate composite carrier	359:400	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	4	21	theme	immobilized	855:865	arg1	pellets					876:882	the immobilized bacteria pellets	851:882	the immobilized bacteria pellets	851:882	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	5	22	theme	immobilized	1086:1096	arg1	pellets					1107:1113	the immobilized bacteria pellets	1082:1113	the immobilized bacteria pellets	1082:1113	In ADSW treatment, at pH 6, 20 g/L of the immobilized bacteria pellets removed 96.5% of ammonia nitrogen.
34175512	7	23	theme	bacteria	1318:1325	arg1	pellets					1327:1333	the immobilized bacteria pellets	1302:1333	the immobilized bacteria pellets using chitosan-sodium alginate as carrier	1302:1375	Compared with the immobilized bacteria pellets using chitosan-sodium alginate as carrier, the one using mono alginate as carrier had a weaker ability to remove ammonia nitrogen, with a removal efficiency of 67.4%.
34175512	9	24	theme	immobilized	1757:1767	arg1	pellets					1778:1784	the immobilized bacteria pellets	1753:1784	the immobilized bacteria pellets using chitosan-sodium alginate as an embedding agent	1753:1837	To sum up, the immobilized bacteria pellets using chitosan-sodium alginate as an embedding agent have a promising prospect in ammonia nitrogen removal from wastewater.
34175512	0	25	theme	Effective	0:8	arg1	immobilization					10:23	Effective immobilization	0:23	Effective immobilization of Bacillus subtilis in chitosan-sodium alginate composite carrier for ammonia removal from anaerobically digested swine wastewater.	0:156	Effective immobilization of Bacillus subtilis in chitosan-sodium alginate composite carrier for ammonia removal from anaerobically digested swine wastewater.
34175512	2	26	theme	high	482:485	arg1	concentrations					487:500	high concentrations	482:500	high concentrations of ammonia nitrogen	482:520	The heterotrophic bacteria tolerant to high concentrations of ammonia nitrogen were isolated and the conditions for immobilizing bacteria were optimized.
34175512	8	27	theme	main	1506:1509	arg1	mechanism					1511:1519	The main mechanism	1502:1519	The main mechanism	1502:1519	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	8	27	theme	main	1506:1509	arg1	formation					1529:1537	the formation	1525:1537	the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life	1525:1739	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	2	28	theme	tolerant	470:477	arg1	bacteria					461:468	The heterotrophic bacteria	443:468	The heterotrophic bacteria tolerant to high concentrations of ammonia nitrogen	443:520	The heterotrophic bacteria tolerant to high concentrations of ammonia nitrogen were isolated and the conditions for immobilizing bacteria were optimized.
34175512	9	29	theme	chitosan-sodium	1792:1806	arg1	alginate					1808:1815	chitosan-sodium alginate	1792:1815	chitosan-sodium alginate	1792:1815	To sum up, the immobilized bacteria pellets using chitosan-sodium alginate as an embedding agent have a promising prospect in ammonia nitrogen removal from wastewater.
34175512	9	29	theme	chitosan-sodium	1792:1806	arg1	agent					1833:1837	an embedding agent	1820:1837	an embedding agent	1820:1837	To sum up, the immobilized bacteria pellets using chitosan-sodium alginate as an embedding agent have a promising prospect in ammonia nitrogen removal from wastewater.
34175512	9	30	contain	have	1839:1842	arg1	pellets					1778:1784	the immobilized bacteria pellets	1753:1784	the immobilized bacteria pellets using chitosan-sodium alginate as an embedding agent	1753:1837	To sum up, the immobilized bacteria pellets using chitosan-sodium alginate as an embedding agent have a promising prospect in ammonia nitrogen removal from wastewater.
34175512	9	30	contain	have	1839:1842	arg2	prospect					1856:1863	a promising prospect	1844:1863	a promising prospect	1844:1863	To sum up, the immobilized bacteria pellets using chitosan-sodium alginate as an embedding agent have a promising prospect in ammonia nitrogen removal from wastewater.
34175512	4	31	theme	methodology	904:914	arg1	tests					916:920	response surface methodology tests	887:920	response surface methodology tests	887:920	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	2	32	theme	heterotrophic	447:459	arg1	bacteria					461:468	The heterotrophic bacteria	443:468	The heterotrophic bacteria tolerant to high concentrations of ammonia nitrogen	443:520	The heterotrophic bacteria tolerant to high concentrations of ammonia nitrogen were isolated and the conditions for immobilizing bacteria were optimized.
34175512	0	33	theme	subtilis	37:44	arg1	immobilization					10:23	Effective immobilization	0:23	Effective immobilization of Bacillus subtilis in chitosan-sodium alginate composite carrier for ammonia removal from anaerobically digested swine wastewater.	0:156	Effective immobilization of Bacillus subtilis in chitosan-sodium alginate composite carrier for ammonia removal from anaerobically digested swine wastewater.
34175512	1	34	theme	swine	325:329	arg1	wastewater					331:340	anaerobically digested swine wastewater	302:340	anaerobically digested swine wastewater	302:340	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	2	35	theme	nitrogen	513:520	arg1	concentrations					487:500	high concentrations	482:500	high concentrations of ammonia nitrogen	482:520	The heterotrophic bacteria tolerant to high concentrations of ammonia nitrogen were isolated and the conditions for immobilizing bacteria were optimized.
34175512	3	36	theme	corresponding	705:717	arg1	mechanism					719:727	the corresponding mechanism	701:727	the corresponding mechanism	701:727	The performance of immobilized bacteria pellets to remove ammonia nitrogen from ADSW was determined and the corresponding mechanism was investigated.
34175512	0	37	theme	chitosan-sodium	49:63	arg1	carrier					84:90	chitosan-sodium alginate composite carrier	49:90	chitosan-sodium alginate composite carrier for ammonia removal from anaerobically digested swine wastewater	49:155	Effective immobilization of Bacillus subtilis in chitosan-sodium alginate composite carrier for ammonia removal from anaerobically digested swine wastewater.
34175512	5	38	theme	nitrogen	1140:1147	arg1	nitrogen					1140:1147	ammonia nitrogen	1132:1147	ammonia nitrogen	1132:1147	In ADSW treatment, at pH 6, 20 g/L of the immobilized bacteria pellets removed 96.5% of ammonia nitrogen.
34175512	5	38	theme	nitrogen	1140:1147	arg1	%					1127:1127	96.5%	1123:1127	96.5% of ammonia nitrogen	1123:1147	In ADSW treatment, at pH 6, 20 g/L of the immobilized bacteria pellets removed 96.5% of ammonia nitrogen.
34175512	9	39	theme	embedding	1823:1831	arg1	alginate					1808:1815	chitosan-sodium alginate	1792:1815	chitosan-sodium alginate	1792:1815	To sum up, the immobilized bacteria pellets using chitosan-sodium alginate as an embedding agent have a promising prospect in ammonia nitrogen removal from wastewater.
34175512	9	39	theme	embedding	1823:1831	arg1	agent					1833:1837	an embedding agent	1820:1837	an embedding agent	1820:1837	To sum up, the immobilized bacteria pellets using chitosan-sodium alginate as an embedding agent have a promising prospect in ammonia nitrogen removal from wastewater.
34175512	8	40	theme	polyelectrolyte	1542:1556	arg1	membrane					1558:1565	polyelectrolyte membrane	1542:1565	polyelectrolyte membrane	1542:1565	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	4	41	theme	response	887:894	arg1	methodology					904:914	response surface methodology	887:914	response surface methodology tests	887:920	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	9	42	theme	promising	1846:1854	arg1	prospect					1856:1863	a promising prospect	1844:1863	a promising prospect	1844:1863	To sum up, the immobilized bacteria pellets using chitosan-sodium alginate as an embedding agent have a promising prospect in ammonia nitrogen removal from wastewater.
34175512	5	43	theme	ammonia	1132:1138	arg1	nitrogen					1140:1147	ammonia nitrogen	1132:1147	ammonia nitrogen	1132:1147	In ADSW treatment, at pH 6, 20 g/L of the immobilized bacteria pellets removed 96.5% of ammonia nitrogen.
34175512	8	44	theme	sodium	1641:1646	arg1	alginate					1648:1655	sodium alginate	1641:1655	sodium alginate	1641:1655	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	1	45	theme	aeration	255:262	arg1	energy					264:269	aeration energy	255:269	aeration energy	255:269	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	7	46	theme	ammonia	1448:1454	arg1	nitrogen					1456:1463	ammonia nitrogen	1448:1463	ammonia nitrogen	1448:1463	Compared with the immobilized bacteria pellets using chitosan-sodium alginate as carrier, the one using mono alginate as carrier had a weaker ability to remove ammonia nitrogen, with a removal efficiency of 67.4%.
34175512	4	47	theme	%	950:950	arg1	%					1035:1035	15%	1033:1035	15% (V/V)	1033:1041	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	47	theme	%	950:950	arg1	m/V					978:980	m/V	978:980	m/V	978:980	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	47	theme	%	950:950	arg1	alginate					934:941	sodium alginate	927:941	sodium alginate of 0.84% (m/V)	927:956	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	47	theme	%	950:950	arg1	32 min					1002:1007	32 min	1002:1007	32 min	1002:1007	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	47	theme	%	950:950	arg1	chitosan					959:966	chitosan	959:966	chitosan of 0.22% (m/V)	959:981	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	47	theme	%	950:950	arg1	V/V					1038:1040	V/V	1038:1040	V/V	1038:1040	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	47	theme	%	950:950	arg1	amount					1023:1028	embedding amount	1013:1028	embedding amount of 15% (V/V)	1013:1041	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	47	theme	%	950:950	arg1	%					950:950	0.84%	946:950	0.84% (m/V)	946:956	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	47	theme	%	950:950	arg1	time					994:997	embedding time	984:997	embedding time of 32 min	984:1007	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	47	theme	%	950:950	arg1	conditions					829:838	the optimal conditions	817:838	the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests	817:920	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	47	theme	%	950:950	arg1	m/V					953:955	m/V	953:955	m/V	953:955	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	47	theme	%	950:950	arg1	%					975:975	0.22%	971:975	0.22% (m/V)	971:981	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	8	48	theme	amino	1593:1597	arg1	groups					1599:1604	amino groups	1593:1604	amino groups of chitosan	1593:1616	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	8	48	theme	amino	1593:1597	arg1	alginate					1648:1655	sodium alginate	1641:1655	sodium alginate	1641:1655	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	8	48	theme	amino	1593:1597	arg1	chitosan					1609:1616	chitosan	1609:1616	chitosan	1609:1616	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	9	49	theme	nitrogen	1876:1883	arg1	removal					1885:1891	ammonia nitrogen removal	1868:1891	ammonia nitrogen removal from wastewater	1868:1907	To sum up, the immobilized bacteria pellets using chitosan-sodium alginate as an embedding agent have a promising prospect in ammonia nitrogen removal from wastewater.
34175512	4	50	theme	%	1035:1035	arg1	%					1035:1035	15%	1033:1035	15% (V/V)	1033:1041	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	50	theme	%	1035:1035	arg1	m/V					978:980	m/V	978:980	m/V	978:980	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	50	theme	%	1035:1035	arg1	alginate					934:941	sodium alginate	927:941	sodium alginate of 0.84% (m/V)	927:956	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	50	theme	%	1035:1035	arg1	32 min					1002:1007	32 min	1002:1007	32 min	1002:1007	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	50	theme	%	1035:1035	arg1	chitosan					959:966	chitosan	959:966	chitosan of 0.22% (m/V)	959:981	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	50	theme	%	1035:1035	arg1	V/V					1038:1040	V/V	1038:1040	V/V	1038:1040	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	50	theme	%	1035:1035	arg1	amount					1023:1028	embedding amount	1013:1028	embedding amount of 15% (V/V)	1013:1041	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	50	theme	%	1035:1035	arg1	%					950:950	0.84%	946:950	0.84% (m/V)	946:956	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	50	theme	%	1035:1035	arg1	time					994:997	embedding time	984:997	embedding time of 32 min	984:1007	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	50	theme	%	1035:1035	arg1	conditions					829:838	the optimal conditions	817:838	the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests	817:920	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	50	theme	%	1035:1035	arg1	m/V					953:955	m/V	953:955	m/V	953:955	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	50	theme	%	1035:1035	arg1	%					975:975	0.22%	971:975	0.22% (m/V)	971:981	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	7	51	theme	weaker	1423:1428	arg1	ability					1430:1436	a weaker ability	1421:1436	a weaker ability to remove ammonia nitrogen	1421:1463	Compared with the immobilized bacteria pellets using chitosan-sodium alginate as carrier, the one using mono alginate as carrier had a weaker ability to remove ammonia nitrogen, with a removal efficiency of 67.4%.
34175512	4	52	theme	sodium	927:932	arg1	conditions					829:838	the optimal conditions	817:838	the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests	817:920	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	52	theme	sodium	927:932	arg1	alginate					934:941	sodium alginate	927:941	sodium alginate of 0.84% (m/V)	927:956	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	3	53	theme	pellets	637:643	arg1	performance					601:611	The performance	597:611	The performance of immobilized bacteria pellets to remove ammonia nitrogen from ADSW	597:680	The performance of immobilized bacteria pellets to remove ammonia nitrogen from ADSW was determined and the corresponding mechanism was investigated.
34175512	8	54	theme	immobilized	1679:1689	arg1	pellets					1700:1706	the immobilized bacteria pellets	1675:1706	the immobilized bacteria pellets	1675:1706	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	3	55	theme	immobilized	616:626	arg1	pellets					637:643	immobilized bacteria pellets	616:643	immobilized bacteria pellets	616:643	The performance of immobilized bacteria pellets to remove ammonia nitrogen from ADSW was determined and the corresponding mechanism was investigated.
34175512	1	56	theme	mass	206:209	arg1	production					211:220	the mass production	202:220	the mass production of sludge	202:230	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	4	57	theme	%	975:975	arg1	%					1035:1035	15%	1033:1035	15% (V/V)	1033:1041	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	57	theme	%	975:975	arg1	m/V					978:980	m/V	978:980	m/V	978:980	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	57	theme	%	975:975	arg1	alginate					934:941	sodium alginate	927:941	sodium alginate of 0.84% (m/V)	927:956	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	57	theme	%	975:975	arg1	32 min					1002:1007	32 min	1002:1007	32 min	1002:1007	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	57	theme	%	975:975	arg1	chitosan					959:966	chitosan	959:966	chitosan of 0.22% (m/V)	959:981	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	57	theme	%	975:975	arg1	V/V					1038:1040	V/V	1038:1040	V/V	1038:1040	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	57	theme	%	975:975	arg1	amount					1023:1028	embedding amount	1013:1028	embedding amount of 15% (V/V)	1013:1041	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	57	theme	%	975:975	arg1	%					950:950	0.84%	946:950	0.84% (m/V)	946:956	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	57	theme	%	975:975	arg1	time					994:997	embedding time	984:997	embedding time of 32 min	984:1007	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	57	theme	%	975:975	arg1	conditions					829:838	the optimal conditions	817:838	the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests	817:920	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	57	theme	%	975:975	arg1	m/V					953:955	m/V	953:955	m/V	953:955	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	57	theme	%	975:975	arg1	%					975:975	0.22%	971:975	0.22% (m/V)	971:981	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	1	58	theme	bacteria	425:432	arg1	pellets					434:440	immobilized bacteria pellets	413:440	immobilized bacteria pellets	413:440	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	8	59	theme	service	1728:1734	arg1	life					1736:1739	their service life	1722:1739	their service life	1722:1739	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	4	60	dep	conditions	829:838	arg1	prepare					843:849	prepare	843:849	to prepare the immobilized bacteria pellets by response surface methodology tests	840:920	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	1	61	used	used	354:357	arg2	study					348:352	this study	343:352	this study	343:352	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	6	62	theme	nitrogen	1210:1217	arg1	removal					1219:1225	ammonia nitrogen removal	1202:1225	ammonia nitrogen removal	1202:1225	Both adsorption and microbial action contributed to ammonia nitrogen removal, and their contributions were 54.3% and 42.2%, respectively.
34175512	9	63	from	wastewater	1898:1907	arg1	removal					1885:1891	ammonia nitrogen removal	1868:1891	ammonia nitrogen removal from wastewater	1868:1907	To sum up, the immobilized bacteria pellets using chitosan-sodium alginate as an embedding agent have a promising prospect in ammonia nitrogen removal from wastewater.
34175512	4	64	theme	32 min	1002:1007	arg1	%					1035:1035	15%	1033:1035	15% (V/V)	1033:1041	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	64	theme	32 min	1002:1007	arg1	m/V					978:980	m/V	978:980	m/V	978:980	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	64	theme	32 min	1002:1007	arg1	alginate					934:941	sodium alginate	927:941	sodium alginate of 0.84% (m/V)	927:956	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	64	theme	32 min	1002:1007	arg1	32 min					1002:1007	32 min	1002:1007	32 min	1002:1007	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	64	theme	32 min	1002:1007	arg1	chitosan					959:966	chitosan	959:966	chitosan of 0.22% (m/V)	959:981	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	64	theme	32 min	1002:1007	arg1	V/V					1038:1040	V/V	1038:1040	V/V	1038:1040	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	64	theme	32 min	1002:1007	arg1	amount					1023:1028	embedding amount	1013:1028	embedding amount of 15% (V/V)	1013:1041	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	64	theme	32 min	1002:1007	arg1	%					950:950	0.84%	946:950	0.84% (m/V)	946:956	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	64	theme	32 min	1002:1007	arg1	time					994:997	embedding time	984:997	embedding time of 32 min	984:1007	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	64	theme	32 min	1002:1007	arg1	conditions					829:838	the optimal conditions	817:838	the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests	817:920	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	64	theme	32 min	1002:1007	arg1	m/V					953:955	m/V	953:955	m/V	953:955	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	64	theme	32 min	1002:1007	arg1	%					975:975	0.22%	971:975	0.22% (m/V)	971:981	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	0	65	theme	ammonia	96:102	arg1	removal					104:110	ammonia removal	96:110	ammonia removal from anaerobically digested swine wastewater	96:155	Effective immobilization of Bacillus subtilis in chitosan-sodium alginate composite carrier for ammonia removal from anaerobically digested swine wastewater.
34175512	1	66	theme	easy	174:177	arg1	loss					179:182	the easy loss	170:182	the easy loss of microorganism	170:199	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	4	67	theme	embedding	1013:1021	arg1	%					1035:1035	15%	1033:1035	15% (V/V)	1033:1041	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	67	theme	embedding	1013:1021	arg1	m/V					978:980	m/V	978:980	m/V	978:980	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	67	theme	embedding	1013:1021	arg1	32 min					1002:1007	32 min	1002:1007	32 min	1002:1007	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	67	theme	embedding	1013:1021	arg1	V/V					1038:1040	V/V	1038:1040	V/V	1038:1040	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	67	theme	embedding	1013:1021	arg1	amount					1023:1028	embedding amount	1013:1028	embedding amount of 15% (V/V)	1013:1041	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	67	theme	embedding	1013:1021	arg1	%					950:950	0.84%	946:950	0.84% (m/V)	946:956	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	67	theme	embedding	1013:1021	arg1	m/V					953:955	m/V	953:955	m/V	953:955	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	67	theme	embedding	1013:1021	arg1	%					975:975	0.22%	971:975	0.22% (m/V)	971:981	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	0	68	from	immobilization	10:23	arg1	carrier					84:90	chitosan-sodium alginate composite carrier	49:90	chitosan-sodium alginate composite carrier for ammonia removal from anaerobically digested swine wastewater	49:155	Effective immobilization of Bacillus subtilis in chitosan-sodium alginate composite carrier for ammonia removal from anaerobically digested swine wastewater.
34175512	4	69	theme	isolated	771:778	arg1	bacteria					780:787	the isolated bacteria	767:787	the isolated bacteria	767:787	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	69	theme	isolated	771:778	arg1	subtilis					803:810	Bacillus subtilis	794:810	Bacillus subtilis	794:810	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	1	70	theme	alginate	375:382	arg1	carrier					394:400	chitosan-sodium alginate composite carrier	359:400	chitosan-sodium alginate composite carrier	359:400	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	3	71	attach	remove	648:653	arg1	ADSW					677:680	ADSW	677:680	ADSW	677:680	The performance of immobilized bacteria pellets to remove ammonia nitrogen from ADSW was determined and the corresponding mechanism was investigated.
34175512	3	71	attach	remove	648:653	arg2	performance					601:611	The performance	597:611	The performance of immobilized bacteria pellets to remove ammonia nitrogen from ADSW	597:680	The performance of immobilized bacteria pellets to remove ammonia nitrogen from ADSW was determined and the corresponding mechanism was investigated.
34175512	0	72	theme	digested	131:138	arg1	wastewater					146:155	anaerobically digested swine wastewater	117:155	anaerobically digested swine wastewater	117:155	Effective immobilization of Bacillus subtilis in chitosan-sodium alginate composite carrier for ammonia removal from anaerobically digested swine wastewater.
34175512	7	73	theme	immobilized	1306:1316	arg1	pellets					1327:1333	the immobilized bacteria pellets	1302:1333	the immobilized bacteria pellets using chitosan-sodium alginate as carrier	1302:1375	Compared with the immobilized bacteria pellets using chitosan-sodium alginate as carrier, the one using mono alginate as carrier had a weaker ability to remove ammonia nitrogen, with a removal efficiency of 67.4%.
34175512	2	74	theme	immobilizing	559:570	arg1	bacteria					572:579	immobilizing bacteria	559:579	immobilizing bacteria	559:579	The heterotrophic bacteria tolerant to high concentrations of ammonia nitrogen were isolated and the conditions for immobilizing bacteria were optimized.
34175512	5	75	theme	bacteria	1098:1105	arg1	pellets					1107:1113	the immobilized bacteria pellets	1082:1113	the immobilized bacteria pellets	1082:1113	In ADSW treatment, at pH 6, 20 g/L of the immobilized bacteria pellets removed 96.5% of ammonia nitrogen.
34175512	9	76	theme	bacteria	1769:1776	arg1	pellets					1778:1784	the immobilized bacteria pellets	1753:1784	the immobilized bacteria pellets using chitosan-sodium alginate as an embedding agent	1753:1837	To sum up, the immobilized bacteria pellets using chitosan-sodium alginate as an embedding agent have a promising prospect in ammonia nitrogen removal from wastewater.
34175512	4	77	theme	optimal	821:827	arg1	conditions					829:838	the optimal conditions	817:838	the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests	817:920	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	4	77	theme	optimal	821:827	arg1	alginate					934:941	sodium alginate	927:941	sodium alginate of 0.84% (m/V)	927:956	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	1	78	theme	digested	316:323	arg1	wastewater					331:340	anaerobically digested swine wastewater	302:340	anaerobically digested swine wastewater	302:340	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	4	79	theme	surface	896:902	arg1	methodology					904:914	response surface methodology	887:914	response surface methodology tests	887:920	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	0	80	theme	alginate	65:72	arg1	carrier					84:90	chitosan-sodium alginate composite carrier	49:90	chitosan-sodium alginate composite carrier for ammonia removal from anaerobically digested swine wastewater	49:155	Effective immobilization of Bacillus subtilis in chitosan-sodium alginate composite carrier for ammonia removal from anaerobically digested swine wastewater.
34175512	1	81	theme	wastewater	331:340	arg1	treatment					289:297	biological treatment	278:297	biological treatment of anaerobically digested swine wastewater	278:340	To overcome the easy loss of microorganism, the mass production of sludge and the consumption of aeration energy during biological treatment of anaerobically digested swine wastewater, this study used chitosan-sodium alginate composite carrier to prepare immobilized bacteria pellets.
34175512	7	82	theme	%	1499:1499	arg1	efficiency					1481:1490	a removal efficiency	1471:1490	a removal efficiency of 67.4%	1471:1499	Compared with the immobilized bacteria pellets using chitosan-sodium alginate as carrier, the one using mono alginate as carrier had a weaker ability to remove ammonia nitrogen, with a removal efficiency of 67.4%.
34175512	2	83	theme	ammonia	505:511	arg1	nitrogen					513:520	ammonia nitrogen	505:520	ammonia nitrogen	505:520	The heterotrophic bacteria tolerant to high concentrations of ammonia nitrogen were isolated and the conditions for immobilizing bacteria were optimized.
34175512	8	84	theme	membrane	1558:1565	arg1	mechanism					1511:1519	The main mechanism	1502:1519	The main mechanism	1502:1519	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	8	84	theme	membrane	1558:1565	arg1	formation					1529:1537	the formation	1525:1537	the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life	1525:1739	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	8	85	theme	carboxyl	1622:1629	arg1	groups					1631:1636	carboxyl groups	1622:1636	carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life	1622:1739	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	8	85	theme	carboxyl	1622:1629	arg1	alginate					1648:1655	sodium alginate	1641:1655	sodium alginate	1641:1655	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	8	85	theme	carboxyl	1622:1629	arg1	chitosan					1609:1616	chitosan	1609:1616	chitosan	1609:1616	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	7	86	contain	had	1417:1419	arg1	one					1382:1384	the one	1378:1384	the one using mono alginate as carrier	1378:1415	Compared with the immobilized bacteria pellets using chitosan-sodium alginate as carrier, the one using mono alginate as carrier had a weaker ability to remove ammonia nitrogen, with a removal efficiency of 67.4%.
34175512	7	86	contain	had	1417:1419	arg2	ability					1430:1436	a weaker ability	1421:1436	a weaker ability to remove ammonia nitrogen	1421:1463	Compared with the immobilized bacteria pellets using chitosan-sodium alginate as carrier, the one using mono alginate as carrier had a weaker ability to remove ammonia nitrogen, with a removal efficiency of 67.4%.
34175512	4	87	theme	bacteria	867:874	arg1	pellets					876:882	the immobilized bacteria pellets	851:882	the immobilized bacteria pellets	851:882	Results showed that the isolated bacteria were Bacillus subtilis, and the optimal conditions to prepare the immobilized bacteria pellets by response surface methodology tests were sodium alginate of 0.84% (m/V), chitosan of 0.22% (m/V), embedding time of 32 min and embedding amount of 15% (V/V).
34175512	7	88	theme	removal	1473:1479	arg1	efficiency					1481:1490	a removal efficiency	1471:1490	a removal efficiency of 67.4%	1471:1499	Compared with the immobilized bacteria pellets using chitosan-sodium alginate as carrier, the one using mono alginate as carrier had a weaker ability to remove ammonia nitrogen, with a removal efficiency of 67.4%.
34175512	0	89	from	wastewater	146:155	arg1	removal					104:110	ammonia removal	96:110	ammonia removal from anaerobically digested swine wastewater	96:155	Effective immobilization of Bacillus subtilis in chitosan-sodium alginate composite carrier for ammonia removal from anaerobically digested swine wastewater.
34175512	8	90	theme	alginate	1648:1655	arg1	groups					1631:1636	carboxyl groups	1622:1636	carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life	1622:1739	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	8	90	theme	alginate	1648:1655	arg1	groups					1599:1604	amino groups	1593:1604	amino groups of chitosan	1593:1616	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	8	90	theme	alginate	1648:1655	arg1	alginate					1648:1655	sodium alginate	1641:1655	sodium alginate	1641:1655	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
34175512	8	90	theme	alginate	1648:1655	arg1	chitosan					1609:1616	chitosan	1609:1616	chitosan	1609:1616	The main mechanism was the formation of polyelectrolyte membrane by the connection between amino groups of chitosan and carboxyl groups of sodium alginate, which stabilized the immobilized bacteria pellets and prolonged their service life.
32814104	7	0	used	utilized	1100:1107	arg2	NBPF					1095:1098	NBPF	1095:1098	NBPF	1095:1098	From the results, it is concluded that NBPF utilized as a polymer matrix composite for manufacturing light load automotive components and construction equipment.
32814104	7	1	theme	polymer	1114:1120	arg1	composite					1129:1137	a polymer matrix composite	1112:1137	a polymer matrix composite for manufacturing light load automotive components and construction equipment	1112:1215	From the results, it is concluded that NBPF utilized as a polymer matrix composite for manufacturing light load automotive components and construction equipment.
32814104	4	2	theme	4.72 nm	787:793	arg1	size					779:782	crystallinity size	765:782	crystallinity size of 4.72 nm	765:793	The XRD analysis for the NBPF shows that the crystallinity index of 53.3%and crystallinity size of 4.72 nm.
32814104	4	2	theme	4.72 nm	787:793	arg1	index					747:751	the crystallinity index	729:751	the crystallinity index of 53.3%	729:760	The XRD analysis for the NBPF shows that the crystallinity index of 53.3%and crystallinity size of 4.72 nm.
32814104	5	3	theme	groups	985:990	arg1	existence					905:913	the existence	901:913	the existence of different chemical compositions and their corresponding functional groups	901:990	Thermogravimetric analysis depicted that NBPF can withstand thermally up to 356 °C. FT-IR results proved the existence of different chemical compositions and their corresponding functional groups.
32814104	2	4	theme	Force	514:518	arg1	AFM					532:534	AFM	532:534	AFM	532:534	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	4	theme	Force	514:518	arg1	analysis					403:410	the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis	324:410	analysis	403:410	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	4	theme	Force	514:518	arg1	Microscopy					520:529	Atomic Force Microscopy	507:529	Atomic Force Microscopy (AFM)	507:535	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	6	5	theme	AFM	993:995	arg1	analysis					997:1004	AFM analysis	993:1004	AFM analysis	993:1004	AFM analysis revealed the surface of the fiber found as rough.
32814104	0	6	from	Characterization	0:15	arg1	plants					75:80	Nendran Banana Peduncle plants	51:80	Nendran Banana Peduncle plants	51:80	Characterization of natural cellulosic fibers from Nendran Banana Peduncle plants.
32814104	2	7	theme	Atomic	507:512	arg1	AFM					532:534	AFM	532:534	AFM	532:534	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	7	theme	Atomic	507:512	arg1	analysis					403:410	the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis	324:410	analysis	403:410	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	7	theme	Atomic	507:512	arg1	Microscopy					520:529	Atomic Force Microscopy	507:529	Atomic Force Microscopy (AFM)	507:535	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	8	theme	Nendran	258:264	arg1	NBPF					289:292	NBPF	289:292	NBPF	289:292	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	8	theme	Nendran	258:264	arg1	Fiber					282:286	the Nendran Banana Peduncle Fiber	254:286	the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work	254:558	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	9	theme	Banana	266:271	arg1	NBPF					289:292	NBPF	289:292	NBPF	289:292	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	9	theme	Banana	266:271	arg1	Fiber					282:286	the Nendran Banana Peduncle Fiber	254:286	the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work	254:558	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	3	10	theme	diameter	627:634	arg1	analysis					615:622	the analysis	611:622	the analysis of diameter, tensile strength and Young's modulus of the fiber	611:685	The Weibull distribution analysis was adopted for the analysis of diameter, tensile strength and Young's modulus of the fiber.
32814104	7	11	theme	matrix	1122:1127	arg1	composite					1129:1137	a polymer matrix composite	1112:1137	a polymer matrix composite for manufacturing light load automotive components and construction equipment	1112:1215	From the results, it is concluded that NBPF utilized as a polymer matrix composite for manufacturing light load automotive components and construction equipment.
32814104	7	12	theme	light	1157:1161	arg1	components					1179:1188	light load automotive components	1157:1188	light load automotive components	1157:1188	From the results, it is concluded that NBPF utilized as a polymer matrix composite for manufacturing light load automotive components and construction equipment.
32814104	7	13	theme	load	1163:1166	arg1	components					1179:1188	light load automotive components	1157:1188	light load automotive components	1157:1188	From the results, it is concluded that NBPF utilized as a polymer matrix composite for manufacturing light load automotive components and construction equipment.
32814104	2	14	theme	furnished	537:545	arg1	analysis					403:410	the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis	324:410	analysis	403:410	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	14	theme	furnished	537:545	arg1	FT-IR					453:457	FT-IR	453:457	FT-IR	453:457	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	14	theme	furnished	537:545	arg1	spectroscopy					440:451	Fourier-transform Infrared spectroscopy	413:451	Fourier-transform Infrared spectroscopy(FT-IR)	413:458	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	15	theme	Diffraction	385:395	arg1	analysis					403:410	the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis	324:410	analysis	403:410	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	15	theme	Diffraction	385:395	arg1	Microscopy					520:529	Atomic Force Microscopy	507:529	Atomic Force Microscopy (AFM)	507:535	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	15	theme	Diffraction	385:395	arg1	spectroscopy					440:451	Fourier-transform Infrared spectroscopy	413:451	Fourier-transform Infrared spectroscopy(FT-IR)	413:458	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	15	theme	Diffraction	385:395	arg1	analysis					494:501	Nuclear Magnetic Resonance (NMR) analysis	461:501	Nuclear Magnetic Resonance (NMR) analysis	461:501	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	1	16	theme	Nendran	169:175	arg1	plants					193:198	Nendran Banana Peduncle plants	169:198	Nendran Banana Peduncle plants	169:198	The objective of this work is to explore the natural cellulosic fibers extracted from Nendran Banana Peduncle plants.
32814104	2	17	theme	X-ray	379:383	arg1	analysis					403:410	the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis	324:410	analysis	403:410	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	17	theme	X-ray	379:383	arg1	Microscopy					520:529	Atomic Force Microscopy	507:529	Atomic Force Microscopy (AFM)	507:535	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	17	theme	X-ray	379:383	arg1	spectroscopy					440:451	Fourier-transform Infrared spectroscopy	413:451	Fourier-transform Infrared spectroscopy(FT-IR)	413:458	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	17	theme	X-ray	379:383	arg1	analysis					494:501	Nuclear Magnetic Resonance (NMR) analysis	461:501	Nuclear Magnetic Resonance (NMR) analysis	461:501	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	4	18	theme	crystallinity	733:745	arg1	index					747:751	the crystallinity index	729:751	the crystallinity index of 53.3%	729:760	The XRD analysis for the NBPF shows that the crystallinity index of 53.3%and crystallinity size of 4.72 nm.
32814104	4	19	dep	shows	718:722	arg1	size					779:782	crystallinity size	765:782	crystallinity size of 4.72 nm	765:793	The XRD analysis for the NBPF shows that the crystallinity index of 53.3%and crystallinity size of 4.72 nm.
32814104	4	19	dep	shows	718:722	arg1	index					747:751	the crystallinity index	729:751	the crystallinity index of 53.3%	729:760	The XRD analysis for the NBPF shows that the crystallinity index of 53.3%and crystallinity size of 4.72 nm.
32814104	1	20	theme	Banana	177:182	arg1	plants					193:198	Nendran Banana Peduncle plants	169:198	Nendran Banana Peduncle plants	169:198	The objective of this work is to explore the natural cellulosic fibers extracted from Nendran Banana Peduncle plants.
32814104	5	21	theme	chemical	928:935	arg1	compositions					937:948	different chemical compositions	918:948	different chemical compositions	918:948	Thermogravimetric analysis depicted that NBPF can withstand thermally up to 356 °C. FT-IR results proved the existence of different chemical compositions and their corresponding functional groups.
32814104	1	22	theme	Peduncle	184:191	arg1	plants					193:198	Nendran Banana Peduncle plants	169:198	Nendran Banana Peduncle plants	169:198	The objective of this work is to explore the natural cellulosic fibers extracted from Nendran Banana Peduncle plants.
32814104	0	23	theme	cellulosic	28:37	arg1	fibers					39:44	natural cellulosic fibers	20:44	natural cellulosic fibers from Nendran Banana Peduncle plants	20:80	Characterization of natural cellulosic fibers from Nendran Banana Peduncle plants.
32814104	3	24	theme	Young	658:662	arg1	modulus					666:672	Young's modulus	658:672	Young's modulus	658:672	The Weibull distribution analysis was adopted for the analysis of diameter, tensile strength and Young's modulus of the fiber.
32814104	2	25	theme	Resonance	478:486	arg1	analysis					403:410	the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis	324:410	analysis	403:410	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	25	theme	Resonance	478:486	arg1	analysis					494:501	Nuclear Magnetic Resonance (NMR) analysis	461:501	Nuclear Magnetic Resonance (NMR) analysis	461:501	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	5	26	theme	different	918:926	arg1	compositions					937:948	different chemical compositions	918:948	different chemical compositions	918:948	Thermogravimetric analysis depicted that NBPF can withstand thermally up to 356 °C. FT-IR results proved the existence of different chemical compositions and their corresponding functional groups.
32814104	2	27	theme	Peduncle	273:280	arg1	NBPF					289:292	NBPF	289:292	NBPF	289:292	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	27	theme	Peduncle	273:280	arg1	Fiber					282:286	the Nendran Banana Peduncle Fiber	254:286	the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work	254:558	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	7	28	theme	construction	1194:1205	arg1	equipment					1207:1215	construction equipment	1194:1215	construction equipment	1194:1215	From the results, it is concluded that NBPF utilized as a polymer matrix composite for manufacturing light load automotive components and construction equipment.
32814104	0	29	theme	natural	20:26	arg1	fibers					39:44	natural cellulosic fibers	20:44	natural cellulosic fibers from Nendran Banana Peduncle plants	20:80	Characterization of natural cellulosic fibers from Nendran Banana Peduncle plants.
32814104	3	30	theme	tensile	637:643	arg1	strength					645:652	tensile strength	637:652	tensile strength	637:652	The Weibull distribution analysis was adopted for the analysis of diameter, tensile strength and Young's modulus of the fiber.
32814104	2	31	theme	Magnetic	469:476	arg1	analysis					403:410	the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis	324:410	analysis	403:410	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	31	theme	Magnetic	469:476	arg1	analysis					494:501	Nuclear Magnetic Resonance (NMR) analysis	461:501	Nuclear Magnetic Resonance (NMR) analysis	461:501	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	5	32	theme	corresponding	960:972	arg1	groups					985:990	their corresponding functional groups	954:990	their corresponding functional groups	954:990	Thermogravimetric analysis depicted that NBPF can withstand thermally up to 356 °C. FT-IR results proved the existence of different chemical compositions and their corresponding functional groups.
32814104	1	33	theme	work	105:108	arg1	objective					87:95	The objective	83:95	The objective of this work	83:108	The objective of this work is to explore the natural cellulosic fibers extracted from Nendran Banana Peduncle plants.
32814104	7	34	theme	automotive	1168:1177	arg1	components					1179:1188	light load automotive components	1157:1188	light load automotive components	1157:1188	From the results, it is concluded that NBPF utilized as a polymer matrix composite for manufacturing light load automotive components and construction equipment.
32814104	2	35	theme	Nuclear	461:467	arg1	analysis					403:410	the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis	324:410	analysis	403:410	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	35	theme	Nuclear	461:467	arg1	analysis					494:501	Nuclear Magnetic Resonance (NMR) analysis	461:501	Nuclear Magnetic Resonance (NMR) analysis	461:501	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	5	36	theme	functional	974:983	arg1	groups					985:990	their corresponding functional groups	954:990	their corresponding functional groups	954:990	Thermogravimetric analysis depicted that NBPF can withstand thermally up to 356 °C. FT-IR results proved the existence of different chemical compositions and their corresponding functional groups.
32814104	2	37	theme	chemical	328:335	arg1	analysis					403:410	the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis	324:410	analysis	403:410	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	37	theme	chemical	328:335	arg1	Microscopy					520:529	Atomic Force Microscopy	507:529	Atomic Force Microscopy (AFM)	507:535	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	37	theme	chemical	328:335	arg1	spectroscopy					440:451	Fourier-transform Infrared spectroscopy	413:451	Fourier-transform Infrared spectroscopy(FT-IR)	413:458	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	37	theme	chemical	328:335	arg1	analysis					494:501	Nuclear Magnetic Resonance (NMR) analysis	461:501	Nuclear Magnetic Resonance (NMR) analysis	461:501	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	38	theme	XRD	398:400	arg1	analysis					403:410	the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis	324:410	analysis	403:410	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	38	theme	XRD	398:400	arg1	Microscopy					520:529	Atomic Force Microscopy	507:529	Atomic Force Microscopy (AFM)	507:535	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	38	theme	XRD	398:400	arg1	spectroscopy					440:451	Fourier-transform Infrared spectroscopy	413:451	Fourier-transform Infrared spectroscopy(FT-IR)	413:458	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	38	theme	XRD	398:400	arg1	analysis					494:501	Nuclear Magnetic Resonance (NMR) analysis	461:501	Nuclear Magnetic Resonance (NMR) analysis	461:501	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	0	39	theme	fibers	39:44	arg1	Characterization					0:15	Characterization	0:15	Characterization of natural cellulosic fibers from Nendran Banana Peduncle plants.	0:81	Characterization of natural cellulosic fibers from Nendran Banana Peduncle plants.
32814104	0	40	from	plants	75:80	arg1	Characterization					0:15	Characterization	0:15	Characterization of natural cellulosic fibers from Nendran Banana Peduncle plants.	0:81	Characterization of natural cellulosic fibers from Nendran Banana Peduncle plants.
32814104	0	40	from	plants	75:80	arg1	fibers					39:44	natural cellulosic fibers	20:44	natural cellulosic fibers from Nendran Banana Peduncle plants	20:80	Characterization of natural cellulosic fibers from Nendran Banana Peduncle plants.
32814104	0	41	theme	Banana	59:64	arg1	plants					75:80	Nendran Banana Peduncle plants	51:80	Nendran Banana Peduncle plants	51:80	Characterization of natural cellulosic fibers from Nendran Banana Peduncle plants.
32814104	4	42	theme	crystallinity	765:777	arg1	size					779:782	crystallinity size	765:782	crystallinity size of 4.72 nm	765:793	The XRD analysis for the NBPF shows that the crystallinity index of 53.3%and crystallinity size of 4.72 nm.
32814104	2	43	theme	Fourier-transform	413:429	arg1	analysis					403:410	the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis	324:410	analysis	403:410	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	43	theme	Fourier-transform	413:429	arg1	FT-IR					453:457	FT-IR	453:457	FT-IR	453:457	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	43	theme	Fourier-transform	413:429	arg1	spectroscopy					440:451	Fourier-transform Infrared spectroscopy	413:451	Fourier-transform Infrared spectroscopy(FT-IR)	413:458	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	44	theme	first	213:217	arg1	time					219:222	the first time	209:222	the first time	209:222	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	44	theme	first	213:217	arg1	This					201:204	This	201:204	This	201:204	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	6	45	theme	fiber	1034:1038	arg1	surface					1019:1025	the surface	1015:1025	the surface of the fiber found as rough	1015:1053	AFM analysis revealed the surface of the fiber found as rough.
32814104	0	46	theme	Nendran	51:57	arg1	plants					75:80	Nendran Banana Peduncle plants	51:80	Nendran Banana Peduncle plants	51:80	Characterization of natural cellulosic fibers from Nendran Banana Peduncle plants.
32814104	2	47	from	furnished	537:545	arg1	work					555:558	this work	550:558	this work	550:558	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	3	48	theme	distribution	573:584	arg1	analysis					586:593	The Weibull distribution analysis	561:593	The Weibull distribution analysis	561:593	The Weibull distribution analysis was adopted for the analysis of diameter, tensile strength and Young's modulus of the fiber.
32814104	2	49	dep	thermal	360:366	arg1	TGA/DTG					369:375	TGA/DTG	369:375	TGA/DTG	369:375	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	4	50	theme	%	760:760	arg1	size					779:782	crystallinity size	765:782	crystallinity size of 4.72 nm	765:793	The XRD analysis for the NBPF shows that the crystallinity index of 53.3%and crystallinity size of 4.72 nm.
32814104	4	50	theme	%	760:760	arg1	index					747:751	the crystallinity index	729:751	the crystallinity index of 53.3%	729:760	The XRD analysis for the NBPF shows that the crystallinity index of 53.3%and crystallinity size of 4.72 nm.
32814104	2	51	theme	NMR	489:491	arg1	analysis					403:410	the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis	324:410	analysis	403:410	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	51	theme	NMR	489:491	arg1	analysis					494:501	Nuclear Magnetic Resonance (NMR) analysis	461:501	Nuclear Magnetic Resonance (NMR) analysis	461:501	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	5	52	theme	compositions	937:948	arg1	existence					905:913	the existence	901:913	the existence of different chemical compositions and their corresponding functional groups	901:990	Thermogravimetric analysis depicted that NBPF can withstand thermally up to 356 °C. FT-IR results proved the existence of different chemical compositions and their corresponding functional groups.
32814104	5	53	theme	356 °C.	872:878	arg1	results					886:892	356 °C. FT-IR results	872:892	356 °C. FT-IR results proved the existence of different chemical compositions and their corresponding functional groups	872:990	Thermogravimetric analysis depicted that NBPF can withstand thermally up to 356 °C. FT-IR results proved the existence of different chemical compositions and their corresponding functional groups.
32814104	5	54	theme	Thermogravimetric	796:812	arg1	analysis					814:821	Thermogravimetric analysis	796:821	Thermogravimetric analysis	796:821	Thermogravimetric analysis depicted that NBPF can withstand thermally up to 356 °C. FT-IR results proved the existence of different chemical compositions and their corresponding functional groups.
32814104	5	55	theme	FT-IR	880:884	arg1	results					886:892	356 °C. FT-IR results	872:892	356 °C. FT-IR results proved the existence of different chemical compositions and their corresponding functional groups	872:990	Thermogravimetric analysis depicted that NBPF can withstand thermally up to 356 °C. FT-IR results proved the existence of different chemical compositions and their corresponding functional groups.
32814104	2	56	from	work	555:558	arg1	furnished					537:545	furnished	537:545	furnished	537:545	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	57	theme	Infrared	431:438	arg1	analysis					403:410	the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis	324:410	analysis	403:410	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	57	theme	Infrared	431:438	arg1	FT-IR					453:457	FT-IR	453:457	FT-IR	453:457	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	57	theme	Infrared	431:438	arg1	spectroscopy					440:451	Fourier-transform Infrared spectroscopy	413:451	Fourier-transform Infrared spectroscopy(FT-IR)	413:458	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	3	58	theme	Weibull	565:571	arg1	analysis					586:593	The Weibull distribution analysis	561:593	The Weibull distribution analysis	561:593	The Weibull distribution analysis was adopted for the analysis of diameter, tensile strength and Young's modulus of the fiber.
32814104	4	59	theme	XRD	692:694	arg1	analysis					696:703	The XRD analysis	688:703	The XRD analysis for the NBPF	688:716	The XRD analysis for the NBPF shows that the crystallinity index of 53.3%and crystallinity size of 4.72 nm.
32814104	1	60	theme	natural	128:134	arg1	fibers					147:152	the natural cellulosic fibers	124:152	the natural cellulosic fibers extracted from Nendran Banana Peduncle plants	124:198	The objective of this work is to explore the natural cellulosic fibers extracted from Nendran Banana Peduncle plants.
32814104	2	61	theme	physical	338:345	arg1	analysis					403:410	the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis	324:410	analysis	403:410	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	61	theme	physical	338:345	arg1	Microscopy					520:529	Atomic Force Microscopy	507:529	Atomic Force Microscopy (AFM)	507:535	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	61	theme	physical	338:345	arg1	spectroscopy					440:451	Fourier-transform Infrared spectroscopy	413:451	Fourier-transform Infrared spectroscopy(FT-IR)	413:458	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	61	theme	physical	338:345	arg1	analysis					494:501	Nuclear Magnetic Resonance (NMR) analysis	461:501	Nuclear Magnetic Resonance (NMR) analysis	461:501	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	3	62	theme	fiber	681:685	arg1	diameter					627:634	diameter	627:634	diameter	627:634	The Weibull distribution analysis was adopted for the analysis of diameter, tensile strength and Young's modulus of the fiber.
32814104	0	63	theme	Peduncle	66:73	arg1	plants					75:80	Nendran Banana Peduncle plants	51:80	Nendran Banana Peduncle plants	51:80	Characterization of natural cellulosic fibers from Nendran Banana Peduncle plants.
32814104	2	64	dep	physical	338:345	arg1	thermal					360:366	thermal	360:366	thermal	360:366	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	2	64	dep	physical	338:345	arg1	mechanical					348:357	mechanical	348:357	mechanical	348:357	This is the first time, the tests are carried out in the Nendran Banana Peduncle Fiber (NBPF) to measure the properties of the chemical, physical, mechanical, thermal (TGA/DTG), X-ray Diffraction (XRD) analysis, Fourier-transform Infrared spectroscopy(FT-IR), Nuclear Magnetic Resonance (NMR) analysis and Atomic Force Microscopy (AFM) furnished in this work.
32814104	1	65	theme	cellulosic	136:145	arg1	fibers					147:152	the natural cellulosic fibers	124:152	the natural cellulosic fibers extracted from Nendran Banana Peduncle plants	124:198	The objective of this work is to explore the natural cellulosic fibers extracted from Nendran Banana Peduncle plants.
34536475	4	0	theme	collaborative	892:904	arg1	effect					906:911	the collaborative effect	888:911	the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination	888:1007	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	2	1	theme	graphene	346:353	arg1	oxide					355:359	Janus graphene oxide	340:359	functionalized Janus graphene oxide (GO) nanosheets	325:375	In this manuscript, the fabrication of functionalized Janus graphene oxide (GO) nanosheets were used by Pickering emulsion template.
34536475	4	2	theme	bonds	973:977	arg1	effect					906:911	the collaborative effect	888:911	the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination	888:1007	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	5	3	contain	have	1126:1129	arg1	motions					1111:1117	human motions	1105:1117	human motions	1105:1117	Moreover, the hydrogels exhibited strain sensitivity and could be able to monitor a variety of human motions, which have outstanding application prospects in wearable flexible sensors.
34536475	5	3	contain	have	1126:1129	arg2	prospects					1155:1163	outstanding application prospects	1131:1163	outstanding application prospects	1131:1163	Moreover, the hydrogels exhibited strain sensitivity and could be able to monitor a variety of human motions, which have outstanding application prospects in wearable flexible sensors.
34536475	2	4	theme	Janus	340:344	arg1	oxide					355:359	Janus graphene oxide	340:359	functionalized Janus graphene oxide (GO) nanosheets	325:375	In this manuscript, the fabrication of functionalized Janus graphene oxide (GO) nanosheets were used by Pickering emulsion template.
34536475	4	5	theme	coordination	996:1007	arg1	effect					906:911	the collaborative effect	888:911	the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination	888:1007	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	0	6	theme	strain	82:87	arg1	sensors					89:95	wearable strain sensors	73:95	wearable strain sensors for human motion detection based on Janus graphene oxide	73:152	Stretchable, rapid self-healing guar gum-poly(acrylic acid) hydrogels as wearable strain sensors for human motion detection based on Janus graphene oxide.
34536475	3	7	theme	Janus	612:616	arg1	gum-poly					626:633	Janus NS/guar gum-poly	612:633	Janus NS/guar gum-poly(acrylic acid)	612:647	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	3	7	theme	Janus	612:616	arg1	acid					643:646	acrylic acid	635:646	acrylic acid	635:646	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	5	8	theme	human	1105:1109	arg1	motions					1111:1117	human motions	1105:1117	human motions	1105:1117	Moreover, the hydrogels exhibited strain sensitivity and could be able to monitor a variety of human motions, which have outstanding application prospects in wearable flexible sensors.
34536475	4	9	theme	metal-ligand	983:994	arg1	coordination					996:1007	metal-ligand coordination	983:1007	metal-ligand coordination	983:1007	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	5	10	theme	outstanding	1131:1141	arg1	prospects					1155:1163	outstanding application prospects	1131:1163	outstanding application prospects	1131:1163	Moreover, the hydrogels exhibited strain sensitivity and could be able to monitor a variety of human motions, which have outstanding application prospects in wearable flexible sensors.
34536475	0	11	theme	wearable	73:80	arg1	sensors					89:95	wearable strain sensors	73:95	wearable strain sensors for human motion detection based on Janus graphene oxide	73:152	Stretchable, rapid self-healing guar gum-poly(acrylic acid) hydrogels as wearable strain sensors for human motion detection based on Janus graphene oxide.
34536475	5	12	theme	flexible	1177:1184	arg1	sensors					1186:1192	wearable flexible sensors	1168:1192	wearable flexible sensors	1168:1192	Moreover, the hydrogels exhibited strain sensitivity and could be able to monitor a variety of human motions, which have outstanding application prospects in wearable flexible sensors.
34536475	4	13	theme	improved	714:721	arg1	efficiency					736:745	The outstandingly improved self-healing efficiency	696:745	The outstandingly improved self-healing efficiency (92.8% for 2 h)	696:761	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	5	14	theme	application	1143:1153	arg1	prospects					1155:1163	outstanding application prospects	1131:1163	outstanding application prospects	1131:1163	Moreover, the hydrogels exhibited strain sensitivity and could be able to monitor a variety of human motions, which have outstanding application prospects in wearable flexible sensors.
34536475	4	15	dep	efficiency	736:745	arg1	toughness					815:823	toughness	815:823	toughness of 873.8%	815:833	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	4	15	dep	efficiency	736:745	arg1	%					752:752	92.8%	748:752	92.8% for 2 h	748:760	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	4	15	dep	efficiency	736:745	arg1	strength					790:797	strength	790:797	strength of 4.12 MPa	790:809	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	4	16	theme	reversible	916:925	arg1	interactions					941:952	reversible electrostatic interactions	916:952	reversible electrostatic interactions	916:952	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	5	17	theme	strain	1044:1049	arg1	sensitivity					1051:1061	strain sensitivity	1044:1061	strain sensitivity	1044:1061	Moreover, the hydrogels exhibited strain sensitivity and could be able to monitor a variety of human motions, which have outstanding application prospects in wearable flexible sensors.
34536475	2	18	dep	oxide	355:359	arg1	GO					362:363	GO	362:363	GO	362:363	In this manuscript, the fabrication of functionalized Janus graphene oxide (GO) nanosheets were used by Pickering emulsion template.
34536475	1	19	theme	Wearable	155:162	arg1	sensors					171:177	Wearable strain sensors	155:177	Wearable strain sensors	155:177	Wearable strain sensors have received widespread attention in research fields due to their applications in human motion detection.
34536475	3	20	theme	acrylic	635:641	arg1	gum-poly					626:633	Janus NS/guar gum-poly	612:633	Janus NS/guar gum-poly(acrylic acid)	612:647	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	3	20	theme	acrylic	635:641	arg1	acid					643:646	acrylic acid	635:646	acrylic acid	635:646	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	4	21	theme	mechanical	767:776	arg1	properties					778:787	mechanical properties	767:787	mechanical properties	767:787	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	4	22	theme	4.12 MPa	802:809	arg1	toughness					815:823	toughness	815:823	toughness of 873.8%	815:833	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	4	22	theme	4.12 MPa	802:809	arg1	strength					790:797	strength	790:797	strength of 4.12 MPa	790:809	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	1	23	theme	strain	164:169	arg1	sensors					171:177	Wearable strain sensors	155:177	Wearable strain sensors	155:177	Wearable strain sensors have received widespread attention in research fields due to their applications in human motion detection.
34536475	4	24	theme	interactions	941:952	arg1	effect					906:911	the collaborative effect	888:911	the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination	888:1007	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	1	25	theme	human	262:266	arg1	detection					275:283	human motion detection	262:283	human motion detection	262:283	Wearable strain sensors have received widespread attention in research fields due to their applications in human motion detection.
34536475	0	26	theme	self-healing	19:30	arg1	gum-poly					37:44	rapid self-healing guar gum-poly	13:44	rapid self-healing guar gum-poly(acrylic acid)	13:58	Stretchable, rapid self-healing guar gum-poly(acrylic acid) hydrogels as wearable strain sensors for human motion detection based on Janus graphene oxide.
34536475	0	26	theme	self-healing	19:30	arg1	acid					54:57	acrylic acid	46:57	acrylic acid	46:57	Stretchable, rapid self-healing guar gum-poly(acrylic acid) hydrogels as wearable strain sensors for human motion detection based on Janus graphene oxide.
34536475	0	26	theme	self-healing	19:30	arg1	Stretchable					0:10	Stretchable	0:10	Stretchable	0:10	Stretchable, rapid self-healing guar gum-poly(acrylic acid) hydrogels as wearable strain sensors for human motion detection based on Janus graphene oxide.
34536475	1	27	theme	motion	268:273	arg1	detection					275:283	human motion detection	262:283	human motion detection	262:283	Wearable strain sensors have received widespread attention in research fields due to their applications in human motion detection.
34536475	0	28	theme	motion	107:112	arg1	detection					114:122	human motion detection	101:122	human motion detection	101:122	Stretchable, rapid self-healing guar gum-poly(acrylic acid) hydrogels as wearable strain sensors for human motion detection based on Janus graphene oxide.
34536475	2	29	theme	emulsion	400:407	arg1	template					409:416	Pickering emulsion template	390:416	Pickering emulsion template	390:416	In this manuscript, the fabrication of functionalized Janus graphene oxide (GO) nanosheets were used by Pickering emulsion template.
34536475	0	30	theme	rapid	13:17	arg1	gum-poly					37:44	rapid self-healing guar gum-poly	13:44	rapid self-healing guar gum-poly(acrylic acid)	13:58	Stretchable, rapid self-healing guar gum-poly(acrylic acid) hydrogels as wearable strain sensors for human motion detection based on Janus graphene oxide.
34536475	0	30	theme	rapid	13:17	arg1	acid					54:57	acrylic acid	46:57	acrylic acid	46:57	Stretchable, rapid self-healing guar gum-poly(acrylic acid) hydrogels as wearable strain sensors for human motion detection based on Janus graphene oxide.
34536475	0	30	theme	rapid	13:17	arg1	Stretchable					0:10	Stretchable	0:10	Stretchable	0:10	Stretchable, rapid self-healing guar gum-poly(acrylic acid) hydrogels as wearable strain sensors for human motion detection based on Janus graphene oxide.
34536475	5	31	theme	motions	1111:1117	arg1	variety					1094:1100	a variety	1092:1100	a variety of human motions, which have outstanding application prospects in wearable flexible sensors	1092:1192	Moreover, the hydrogels exhibited strain sensitivity and could be able to monitor a variety of human motions, which have outstanding application prospects in wearable flexible sensors.
34536475	5	31	theme	motions	1111:1117	arg1	motions					1111:1117	human motions	1105:1117	human motions	1105:1117	Moreover, the hydrogels exhibited strain sensitivity and could be able to monitor a variety of human motions, which have outstanding application prospects in wearable flexible sensors.
34536475	0	32	theme	human	101:105	arg1	detection					114:122	human motion detection	101:122	human motion detection	101:122	Stretchable, rapid self-healing guar gum-poly(acrylic acid) hydrogels as wearable strain sensors for human motion detection based on Janus graphene oxide.
34536475	3	33	theme	ethyl	463:467	arg1	methacrylate					469:480	2-(dimethylamino)ethyl methacrylate	446:480	2-(dimethylamino)ethyl methacrylate	446:480	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	3	33	theme	ethyl	463:467	arg1	poly					441:444	poly	441:444	poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA)	441:491	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	2	34	theme	Pickering	390:398	arg1	template					409:416	Pickering emulsion template	390:416	Pickering emulsion template	390:416	In this manuscript, the fabrication of functionalized Janus graphene oxide (GO) nanosheets were used by Pickering emulsion template.
34536475	4	35	theme	hydrogen	964:971	arg1	bonds					973:977	multiple hydrogen bonds	955:977	multiple hydrogen bonds	955:977	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	1	36	theme	widespread	193:202	arg1	attention					204:212	widespread attention	193:212	widespread attention in research fields due to their applications in human motion detection	193:283	Wearable strain sensors have received widespread attention in research fields due to their applications in human motion detection.
34536475	0	37	theme	guar	32:35	arg1	gum-poly					37:44	rapid self-healing guar gum-poly	13:44	rapid self-healing guar gum-poly(acrylic acid)	13:58	Stretchable, rapid self-healing guar gum-poly(acrylic acid) hydrogels as wearable strain sensors for human motion detection based on Janus graphene oxide.
34536475	0	37	theme	guar	32:35	arg1	acid					54:57	acrylic acid	46:57	acrylic acid	46:57	Stretchable, rapid self-healing guar gum-poly(acrylic acid) hydrogels as wearable strain sensors for human motion detection based on Janus graphene oxide.
34536475	0	37	theme	guar	32:35	arg1	Stretchable					0:10	Stretchable	0:10	Stretchable	0:10	Stretchable, rapid self-healing guar gum-poly(acrylic acid) hydrogels as wearable strain sensors for human motion detection based on Janus graphene oxide.
34536475	3	38	theme	Janus	560:564	arg1	GO					541:542	GO	541:542	GO	541:542	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	3	38	theme	Janus	560:564	arg1	NS					566:567	GO@PPy/PDMAEMA Janus NS	545:567	GO@PPy/PDMAEMA Janus NS	545:567	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	3	39	theme	GO	541:542	arg1	sides					532:536	the two sides	524:536	the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels	524:693	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	2	40	used	used	382:385	arg2	fabrication					310:320	the fabrication	306:320	the fabrication of functionalized Janus graphene oxide (GO) nanosheets	306:375	In this manuscript, the fabrication of functionalized Janus graphene oxide (GO) nanosheets were used by Pickering emulsion template.
34536475	0	41	theme	acrylic	46:52	arg1	gum-poly					37:44	rapid self-healing guar gum-poly	13:44	rapid self-healing guar gum-poly(acrylic acid)	13:58	Stretchable, rapid self-healing guar gum-poly(acrylic acid) hydrogels as wearable strain sensors for human motion detection based on Janus graphene oxide.
34536475	0	41	theme	acrylic	46:52	arg1	acid					54:57	acrylic acid	46:57	acrylic acid	46:57	Stretchable, rapid self-healing guar gum-poly(acrylic acid) hydrogels as wearable strain sensors for human motion detection based on Janus graphene oxide.
34536475	4	42	theme	hydrogels	853:861	arg1	efficiency					736:745	The outstandingly improved self-healing efficiency	696:745	The outstandingly improved self-healing efficiency (92.8% for 2 h)	696:761	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	4	42	theme	hydrogels	853:861	arg1	properties					778:787	mechanical properties	767:787	mechanical properties	767:787	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	0	43	theme	Janus	133:137	arg1	oxide					148:152	Janus graphene oxide	133:152	Janus graphene oxide	133:152	Stretchable, rapid self-healing guar gum-poly(acrylic acid) hydrogels as wearable strain sensors for human motion detection based on Janus graphene oxide.
34536475	3	44	theme	self-healing	658:669	arg1	hydrogels					685:693	Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels	612:693	Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels	612:693	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	1	45	from	attention	204:212	arg1	fields					226:231	research fields	217:231	research fields	217:231	Wearable strain sensors have received widespread attention in research fields due to their applications in human motion detection.
34536475	2	46	theme	functionalized	325:338	arg1	nanosheets					366:375	functionalized Janus graphene oxide (GO) nanosheets	325:375	functionalized Janus graphene oxide (GO) nanosheets	325:375	In this manuscript, the fabrication of functionalized Janus graphene oxide (GO) nanosheets were used by Pickering emulsion template.
34536475	3	47	dep	self-healing	658:669	arg1	gum-poly					626:633	Janus NS/guar gum-poly	612:633	Janus NS/guar gum-poly(acrylic acid)	612:647	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	3	47	dep	self-healing	658:669	arg1	acid					643:646	acrylic acid	635:646	acrylic acid	635:646	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	3	48	theme	nanocomposite	671:683	arg1	hydrogels					685:693	Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels	612:693	Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels	612:693	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	1	49	from	applications	246:257	arg1	detection					275:283	human motion detection	262:283	human motion detection	262:283	Wearable strain sensors have received widespread attention in research fields due to their applications in human motion detection.
34536475	4	50	theme	self-healing	723:734	arg1	efficiency					736:745	The outstandingly improved self-healing efficiency	696:745	The outstandingly improved self-healing efficiency (92.8% for 2 h)	696:761	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	4	51	theme	multiple	955:962	arg1	bonds					973:977	multiple hydrogen bonds	955:977	multiple hydrogen bonds	955:977	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	3	52	theme	@	547:547	arg1	GO					541:542	GO	541:542	GO	541:542	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	3	52	theme	@	547:547	arg1	NS					566:567	GO@PPy/PDMAEMA Janus NS	545:567	GO@PPy/PDMAEMA Janus NS	545:567	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	0	53	theme	graphene	139:146	arg1	oxide					148:152	Janus graphene oxide	133:152	Janus graphene oxide	133:152	Stretchable, rapid self-healing guar gum-poly(acrylic acid) hydrogels as wearable strain sensors for human motion detection based on Janus graphene oxide.
34536475	3	54	theme	PPy/PDMAEMA	548:558	arg1	GO					541:542	GO	541:542	GO	541:542	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	3	54	theme	PPy/PDMAEMA	548:558	arg1	NS					566:567	GO@PPy/PDMAEMA Janus NS	545:567	GO@PPy/PDMAEMA Janus NS	545:567	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	1	55	theme	research	217:224	arg1	fields					226:231	research fields	217:231	research fields	217:231	Wearable strain sensors have received widespread attention in research fields due to their applications in human motion detection.
34536475	4	56	theme	%	833:833	arg1	toughness					815:823	toughness	815:823	toughness of 873.8%	815:833	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	4	56	theme	%	833:833	arg1	strength					790:797	strength	790:797	strength of 4.12 MPa	790:809	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	2	57	theme	nanosheets	366:375	arg1	fabrication					310:320	the fabrication	306:320	the fabrication of functionalized Janus graphene oxide (GO) nanosheets	306:375	In this manuscript, the fabrication of functionalized Janus graphene oxide (GO) nanosheets were used by Pickering emulsion template.
34536475	5	58	theme	wearable	1168:1175	arg1	sensors					1186:1192	wearable flexible sensors	1168:1192	wearable flexible sensors	1168:1192	Moreover, the hydrogels exhibited strain sensitivity and could be able to monitor a variety of human motions, which have outstanding application prospects in wearable flexible sensors.
34536475	4	59	theme	electrostatic	927:939	arg1	interactions					941:952	reversible electrostatic interactions	916:952	reversible electrostatic interactions	916:952	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
34536475	3	60	theme	NS/guar	618:624	arg1	gum-poly					626:633	Janus NS/guar gum-poly	612:633	Janus NS/guar gum-poly(acrylic acid)	612:647	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	3	60	theme	NS/guar	618:624	arg1	acid					643:646	acrylic acid	635:646	acrylic acid	635:646	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	3	61	theme	GO	545:546	arg1	GO					541:542	GO	541:542	GO	541:542	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	3	61	theme	GO	545:546	arg1	NS					566:567	GO@PPy/PDMAEMA Janus NS	545:567	GO@PPy/PDMAEMA Janus NS	545:567	Polypyrrole (PPy) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were asymmetrically grafted on the two sides of GO (GO@PPy/PDMAEMA Janus NS), which successfully applied to synthesize Janus NS/guar gum-poly(acrylic acid) (GG-PAA) self-healing nanocomposite hydrogels.
34536475	2	62	theme	oxide	355:359	arg1	nanosheets					366:375	functionalized Janus graphene oxide (GO) nanosheets	325:375	functionalized Janus graphene oxide (GO) nanosheets	325:375	In this manuscript, the fabrication of functionalized Janus graphene oxide (GO) nanosheets were used by Pickering emulsion template.
34536475	4	63	theme	nanocomposite	839:851	arg1	hydrogels					853:861	nanocomposite hydrogels	839:861	nanocomposite hydrogels	839:861	The outstandingly improved self-healing efficiency (92.8% for 2 h) and mechanical properties (strength of 4.12 MPa and toughness of 873.8%) of nanocomposite hydrogels were mainly supported by the collaborative effect of reversible electrostatic interactions, multiple hydrogen bonds and metal-ligand coordination.
32420551	7	0	dep	bacteria	1242:1249	arg1	Parabacteroides					1273:1287	Parabacteroides	1273:1287	Parabacteroides	1273:1287	Specifically, UAOS treatment selectively increased the relative abundance of beneficial intestinal bacteria (e.g. Lactobacillus and Akkermansia genus) and decreased the abundance of inflammogenic bacteria (e.g. Bacteroides and Parabacteroides).
32420551	7	0	dep	bacteria	1242:1249	arg1	Bacteroides					1257:1267	e.g. Bacteroides	1252:1267	e.g. Bacteroides	1252:1267	Specifically, UAOS treatment selectively increased the relative abundance of beneficial intestinal bacteria (e.g. Lactobacillus and Akkermansia genus) and decreased the abundance of inflammogenic bacteria (e.g. Bacteroides and Parabacteroides).
32420551	7	1	theme	relative	1101:1108	arg1	abundance					1110:1118	the relative abundance	1097:1118	the relative abundance of beneficial intestinal bacteria (e.g. Lactobacillus and Akkermansia genus)	1097:1195	Specifically, UAOS treatment selectively increased the relative abundance of beneficial intestinal bacteria (e.g. Lactobacillus and Akkermansia genus) and decreased the abundance of inflammogenic bacteria (e.g. Bacteroides and Parabacteroides).
32420551	8	2	theme	potent	1454:1459	arg1	UAOS					1438:1441	UAOS	1438:1441	UAOS	1438:1441	These results suggest that UAOS can attenuate the HFD-induced obesity and related abnormalities through modulating gut microbiota, indicating that UAOS can act as potent prebiotic agents in treating obesity and related metabolic diseases.
32420551	8	2	theme	potent	1454:1459	arg1	agents					1471:1476	potent prebiotic agents	1454:1476	potent prebiotic agents	1454:1476	These results suggest that UAOS can attenuate the HFD-induced obesity and related abnormalities through modulating gut microbiota, indicating that UAOS can act as potent prebiotic agents in treating obesity and related metabolic diseases.
32420551	1	3	theme	high-fat	187:194	arg1	HFD					202:204	HFD	202:204	HFD	202:204	Gut microbiota plays an important role in the high-fat diet (HFD)-induced obesity and related metabolic syndrome (MetS).
32420551	1	3	theme	high-fat	187:194	arg1	diet					196:199	high-fat diet	187:199	high-fat diet (HFD)	187:205	Gut microbiota plays an important role in the high-fat diet (HFD)-induced obesity and related metabolic syndrome (MetS).
32420551	4	4	theme	beneficial	637:646	arg1	effect					648:653	the beneficial effect	633:653	the beneficial effect of UAOS on these metabolic abnormalities	633:694	Particularly, the beneficial effect of UAOS on these metabolic abnormalities could be significantly reversed by antibiotic supplementation.
32420551	5	5	theme	UAOS	820:823	arg1	treatment					825:833	UAOS treatment	820:833	UAOS treatment	820:833	Subsequently, the microbiological analysis has revealed that UAOS treatment can modulate the overall composition of the gut microbiota, which is highly associated with metabolic parameters.
32420551	2	6	theme	alginate	360:367	arg1	lyase					369:373	alginate lyase	360:373	alginate lyase	360:373	Our previous study has demonstrated that unsaturated alginate oligosaccharides (UAOS) degraded by alginate lyase possess significant anti-obesity effects in HFD-fed mice.
32420551	3	7	theme	low-grade	595:603	arg1	inflammation					605:616	low-grade inflammation	595:616	low-grade inflammation	595:616	Herein, we further established that UAOS could significantly ameliorate obesity-related metabolic abnormalities, including hyperlipidemia, insulin resistance and low-grade inflammation.
32420551	1	8	theme	Gut	141:143	arg1	microbiota					145:154	Gut microbiota	141:154	Gut microbiota	141:154	Gut microbiota plays an important role in the high-fat diet (HFD)-induced obesity and related metabolic syndrome (MetS).
32420551	4	9	theme	UAOS	658:661	arg1	effect					648:653	the beneficial effect	633:653	the beneficial effect of UAOS on these metabolic abnormalities	633:694	Particularly, the beneficial effect of UAOS on these metabolic abnormalities could be significantly reversed by antibiotic supplementation.
32420551	0	10	theme	high-fat-diet	121:133	arg1	mice					135:138	high-fat-diet mice	121:138	high-fat-diet mice	121:138	Unsaturated alginate oligosaccharides attenuated obesity-related metabolic abnormalities by modulating gut microbiota in high-fat-diet mice.
32420551	7	11	theme	UAOS	1060:1063	arg1	treatment					1065:1073	UAOS treatment	1060:1073	UAOS treatment	1060:1073	Specifically, UAOS treatment selectively increased the relative abundance of beneficial intestinal bacteria (e.g. Lactobacillus and Akkermansia genus) and decreased the abundance of inflammogenic bacteria (e.g. Bacteroides and Parabacteroides).
32420551	3	12	theme	metabolic	521:529	arg1	abnormalities					531:543	obesity-related metabolic abnormalities	505:543	obesity-related metabolic abnormalities	505:543	Herein, we further established that UAOS could significantly ameliorate obesity-related metabolic abnormalities, including hyperlipidemia, insulin resistance and low-grade inflammation.
32420551	3	12	theme	metabolic	521:529	arg1	hyperlipidemia					556:569	hyperlipidemia	556:569	hyperlipidemia	556:569	Herein, we further established that UAOS could significantly ameliorate obesity-related metabolic abnormalities, including hyperlipidemia, insulin resistance and low-grade inflammation.
32420551	3	12	theme	metabolic	521:529	arg1	resistance					580:589	insulin resistance	572:589	insulin resistance	572:589	Herein, we further established that UAOS could significantly ameliorate obesity-related metabolic abnormalities, including hyperlipidemia, insulin resistance and low-grade inflammation.
32420551	3	12	theme	metabolic	521:529	arg1	inflammation					605:616	low-grade inflammation	595:616	low-grade inflammation	595:616	Herein, we further established that UAOS could significantly ameliorate obesity-related metabolic abnormalities, including hyperlipidemia, insulin resistance and low-grade inflammation.
32420551	4	13	from	effect	648:653	arg1	abnormalities					682:694	these metabolic abnormalities	666:694	these metabolic abnormalities	666:694	Particularly, the beneficial effect of UAOS on these metabolic abnormalities could be significantly reversed by antibiotic supplementation.
32420551	6	14	theme	gut	996:998	arg1	dysbiosis					1000:1008	the gut dysbiosis	992:1008	the gut dysbiosis induced by HFD-diet or antibiotics	992:1043	UAOS supplementation can partially reverse the gut dysbiosis induced by HFD-diet or antibiotics.
32420551	8	15	theme	related	1502:1508	arg1	diseases					1520:1527	related metabolic diseases	1502:1527	related metabolic diseases	1502:1527	These results suggest that UAOS can attenuate the HFD-induced obesity and related abnormalities through modulating gut microbiota, indicating that UAOS can act as potent prebiotic agents in treating obesity and related metabolic diseases.
32420551	2	16	theme	HFD-fed	419:425	arg1	mice					427:430	HFD-fed mice	419:430	HFD-fed mice	419:430	Our previous study has demonstrated that unsaturated alginate oligosaccharides (UAOS) degraded by alginate lyase possess significant anti-obesity effects in HFD-fed mice.
32420551	2	17	contain	possess	375:381	arg1	UAOS					342:345	UAOS	342:345	UAOS	342:345	Our previous study has demonstrated that unsaturated alginate oligosaccharides (UAOS) degraded by alginate lyase possess significant anti-obesity effects in HFD-fed mice.
32420551	2	17	contain	possess	375:381	arg1	oligosaccharides					324:339	unsaturated alginate oligosaccharides	303:339	unsaturated alginate oligosaccharides (UAOS) degraded by alginate lyase	303:373	Our previous study has demonstrated that unsaturated alginate oligosaccharides (UAOS) degraded by alginate lyase possess significant anti-obesity effects in HFD-fed mice.
32420551	2	17	contain	possess	375:381	arg2	effects					408:414	significant anti-obesity effects	383:414	significant anti-obesity effects	383:414	Our previous study has demonstrated that unsaturated alginate oligosaccharides (UAOS) degraded by alginate lyase possess significant anti-obesity effects in HFD-fed mice.
32420551	7	18	theme	inflammogenic	1228:1240	arg1	bacteria					1242:1249	inflammogenic bacteria	1228:1249	inflammogenic bacteria (e.g. Bacteroides and Parabacteroides)	1228:1288	Specifically, UAOS treatment selectively increased the relative abundance of beneficial intestinal bacteria (e.g. Lactobacillus and Akkermansia genus) and decreased the abundance of inflammogenic bacteria (e.g. Bacteroides and Parabacteroides).
32420551	7	19	dep	bacteria	1145:1152	arg1	Lactobacillus					1160:1172	e.g. Lactobacillus	1155:1172	e.g. Lactobacillus	1155:1172	Specifically, UAOS treatment selectively increased the relative abundance of beneficial intestinal bacteria (e.g. Lactobacillus and Akkermansia genus) and decreased the abundance of inflammogenic bacteria (e.g. Bacteroides and Parabacteroides).
32420551	7	19	dep	bacteria	1145:1152	arg1	genus					1190:1194	Akkermansia genus	1178:1194	Akkermansia genus	1178:1194	Specifically, UAOS treatment selectively increased the relative abundance of beneficial intestinal bacteria (e.g. Lactobacillus and Akkermansia genus) and decreased the abundance of inflammogenic bacteria (e.g. Bacteroides and Parabacteroides).
32420551	0	20	theme	alginate	12:19	arg1	oligosaccharides					21:36	Unsaturated alginate oligosaccharides	0:36	Unsaturated alginate oligosaccharides	0:36	Unsaturated alginate oligosaccharides attenuated obesity-related metabolic abnormalities by modulating gut microbiota in high-fat-diet mice.
32420551	8	21	theme	gut	1406:1408	arg1	microbiota					1410:1419	gut microbiota	1406:1419	gut microbiota	1406:1419	These results suggest that UAOS can attenuate the HFD-induced obesity and related abnormalities through modulating gut microbiota, indicating that UAOS can act as potent prebiotic agents in treating obesity and related metabolic diseases.
32420551	0	22	theme	Unsaturated	0:10	arg1	oligosaccharides					21:36	Unsaturated alginate oligosaccharides	0:36	Unsaturated alginate oligosaccharides	0:36	Unsaturated alginate oligosaccharides attenuated obesity-related metabolic abnormalities by modulating gut microbiota in high-fat-diet mice.
32420551	4	23	theme	antibiotic	731:740	arg1	supplementation					742:756	antibiotic supplementation	731:756	antibiotic supplementation	731:756	Particularly, the beneficial effect of UAOS on these metabolic abnormalities could be significantly reversed by antibiotic supplementation.
32420551	8	24	theme	metabolic	1510:1518	arg1	diseases					1520:1527	related metabolic diseases	1502:1527	related metabolic diseases	1502:1527	These results suggest that UAOS can attenuate the HFD-induced obesity and related abnormalities through modulating gut microbiota, indicating that UAOS can act as potent prebiotic agents in treating obesity and related metabolic diseases.
32420551	2	25	theme	anti-obesity	395:406	arg1	effects					408:414	significant anti-obesity effects	383:414	significant anti-obesity effects	383:414	Our previous study has demonstrated that unsaturated alginate oligosaccharides (UAOS) degraded by alginate lyase possess significant anti-obesity effects in HFD-fed mice.
32420551	2	26	theme	alginate	315:322	arg1	UAOS					342:345	UAOS	342:345	UAOS	342:345	Our previous study has demonstrated that unsaturated alginate oligosaccharides (UAOS) degraded by alginate lyase possess significant anti-obesity effects in HFD-fed mice.
32420551	2	26	theme	alginate	315:322	arg1	oligosaccharides					324:339	unsaturated alginate oligosaccharides	303:339	unsaturated alginate oligosaccharides (UAOS) degraded by alginate lyase	303:373	Our previous study has demonstrated that unsaturated alginate oligosaccharides (UAOS) degraded by alginate lyase possess significant anti-obesity effects in HFD-fed mice.
32420551	1	27	theme	-induced	206:213	arg1	obesity					215:221	the high-fat diet (HFD)-induced obesity	183:221	the high-fat diet (HFD)-induced obesity	183:221	Gut microbiota plays an important role in the high-fat diet (HFD)-induced obesity and related metabolic syndrome (MetS).
32420551	2	28	theme	unsaturated	303:313	arg1	UAOS					342:345	UAOS	342:345	UAOS	342:345	Our previous study has demonstrated that unsaturated alginate oligosaccharides (UAOS) degraded by alginate lyase possess significant anti-obesity effects in HFD-fed mice.
32420551	2	28	theme	unsaturated	303:313	arg1	oligosaccharides					324:339	unsaturated alginate oligosaccharides	303:339	unsaturated alginate oligosaccharides (UAOS) degraded by alginate lyase	303:373	Our previous study has demonstrated that unsaturated alginate oligosaccharides (UAOS) degraded by alginate lyase possess significant anti-obesity effects in HFD-fed mice.
32420551	5	29	theme	metabolic	927:935	arg1	parameters					937:946	metabolic parameters	927:946	metabolic parameters	927:946	Subsequently, the microbiological analysis has revealed that UAOS treatment can modulate the overall composition of the gut microbiota, which is highly associated with metabolic parameters.
32420551	0	30	theme	metabolic	65:73	arg1	abnormalities					75:87	obesity-related metabolic abnormalities	49:87	obesity-related metabolic abnormalities	49:87	Unsaturated alginate oligosaccharides attenuated obesity-related metabolic abnormalities by modulating gut microbiota in high-fat-diet mice.
32420551	0	31	from	microbiota	107:116	arg1	mice					135:138	high-fat-diet mice	121:138	high-fat-diet mice	121:138	Unsaturated alginate oligosaccharides attenuated obesity-related metabolic abnormalities by modulating gut microbiota in high-fat-diet mice.
32420551	0	32	theme	obesity-related	49:63	arg1	abnormalities					75:87	obesity-related metabolic abnormalities	49:87	obesity-related metabolic abnormalities	49:87	Unsaturated alginate oligosaccharides attenuated obesity-related metabolic abnormalities by modulating gut microbiota in high-fat-diet mice.
32420551	5	33	theme	gut	879:881	arg1	microbiota					883:892	the gut microbiota	875:892	the gut microbiota	875:892	Subsequently, the microbiological analysis has revealed that UAOS treatment can modulate the overall composition of the gut microbiota, which is highly associated with metabolic parameters.
32420551	7	34	theme	bacteria	1145:1152	arg1	abundance					1110:1118	the relative abundance	1097:1118	the relative abundance of beneficial intestinal bacteria (e.g. Lactobacillus and Akkermansia genus)	1097:1195	Specifically, UAOS treatment selectively increased the relative abundance of beneficial intestinal bacteria (e.g. Lactobacillus and Akkermansia genus) and decreased the abundance of inflammogenic bacteria (e.g. Bacteroides and Parabacteroides).
32420551	1	35	theme	related	227:233	arg1	MetS					255:258	MetS	255:258	MetS	255:258	Gut microbiota plays an important role in the high-fat diet (HFD)-induced obesity and related metabolic syndrome (MetS).
32420551	1	35	theme	related	227:233	arg1	syndrome					245:252	related metabolic syndrome	227:252	related metabolic syndrome (MetS)	227:259	Gut microbiota plays an important role in the high-fat diet (HFD)-induced obesity and related metabolic syndrome (MetS).
32420551	5	36	theme	microbiota	883:892	arg1	composition					860:870	the overall composition	848:870	the overall composition of the gut microbiota, which is highly associated with metabolic parameters	848:946	Subsequently, the microbiological analysis has revealed that UAOS treatment can modulate the overall composition of the gut microbiota, which is highly associated with metabolic parameters.
32420551	8	37	theme	HFD-induced	1341:1351	arg1	obesity					1353:1359	the HFD-induced obesity	1337:1359	the HFD-induced obesity	1337:1359	These results suggest that UAOS can attenuate the HFD-induced obesity and related abnormalities through modulating gut microbiota, indicating that UAOS can act as potent prebiotic agents in treating obesity and related metabolic diseases.
32420551	6	38	theme	UAOS	949:952	arg1	supplementation					954:968	UAOS supplementation	949:968	UAOS supplementation	949:968	UAOS supplementation can partially reverse the gut dysbiosis induced by HFD-diet or antibiotics.
32420551	7	39	theme	beneficial	1123:1132	arg1	bacteria					1145:1152	beneficial intestinal bacteria	1123:1152	beneficial intestinal bacteria (e.g. Lactobacillus and Akkermansia genus)	1123:1195	Specifically, UAOS treatment selectively increased the relative abundance of beneficial intestinal bacteria (e.g. Lactobacillus and Akkermansia genus) and decreased the abundance of inflammogenic bacteria (e.g. Bacteroides and Parabacteroides).
32420551	7	40	theme	bacteria	1242:1249	arg1	abundance					1215:1223	the abundance	1211:1223	the abundance of inflammogenic bacteria (e.g. Bacteroides and Parabacteroides)	1211:1288	Specifically, UAOS treatment selectively increased the relative abundance of beneficial intestinal bacteria (e.g. Lactobacillus and Akkermansia genus) and decreased the abundance of inflammogenic bacteria (e.g. Bacteroides and Parabacteroides).
32420551	2	41	theme	previous	266:273	arg1	study					275:279	Our previous study	262:279	Our previous study	262:279	Our previous study has demonstrated that unsaturated alginate oligosaccharides (UAOS) degraded by alginate lyase possess significant anti-obesity effects in HFD-fed mice.
32420551	7	42	theme	intestinal	1134:1143	arg1	bacteria					1145:1152	beneficial intestinal bacteria	1123:1152	beneficial intestinal bacteria (e.g. Lactobacillus and Akkermansia genus)	1123:1195	Specifically, UAOS treatment selectively increased the relative abundance of beneficial intestinal bacteria (e.g. Lactobacillus and Akkermansia genus) and decreased the abundance of inflammogenic bacteria (e.g. Bacteroides and Parabacteroides).
32420551	3	43	theme	obesity-related	505:519	arg1	abnormalities					531:543	obesity-related metabolic abnormalities	505:543	obesity-related metabolic abnormalities	505:543	Herein, we further established that UAOS could significantly ameliorate obesity-related metabolic abnormalities, including hyperlipidemia, insulin resistance and low-grade inflammation.
32420551	3	43	theme	obesity-related	505:519	arg1	hyperlipidemia					556:569	hyperlipidemia	556:569	hyperlipidemia	556:569	Herein, we further established that UAOS could significantly ameliorate obesity-related metabolic abnormalities, including hyperlipidemia, insulin resistance and low-grade inflammation.
32420551	3	43	theme	obesity-related	505:519	arg1	resistance					580:589	insulin resistance	572:589	insulin resistance	572:589	Herein, we further established that UAOS could significantly ameliorate obesity-related metabolic abnormalities, including hyperlipidemia, insulin resistance and low-grade inflammation.
32420551	3	43	theme	obesity-related	505:519	arg1	inflammation					605:616	low-grade inflammation	595:616	low-grade inflammation	595:616	Herein, we further established that UAOS could significantly ameliorate obesity-related metabolic abnormalities, including hyperlipidemia, insulin resistance and low-grade inflammation.
32420551	1	44	theme	metabolic	235:243	arg1	MetS					255:258	MetS	255:258	MetS	255:258	Gut microbiota plays an important role in the high-fat diet (HFD)-induced obesity and related metabolic syndrome (MetS).
32420551	1	44	theme	metabolic	235:243	arg1	syndrome					245:252	related metabolic syndrome	227:252	related metabolic syndrome (MetS)	227:259	Gut microbiota plays an important role in the high-fat diet (HFD)-induced obesity and related metabolic syndrome (MetS).
32420551	5	45	theme	microbiological	777:791	arg1	analysis					793:800	the microbiological analysis	773:800	the microbiological analysis	773:800	Subsequently, the microbiological analysis has revealed that UAOS treatment can modulate the overall composition of the gut microbiota, which is highly associated with metabolic parameters.
32420551	8	46	theme	prebiotic	1461:1469	arg1	UAOS					1438:1441	UAOS	1438:1441	UAOS	1438:1441	These results suggest that UAOS can attenuate the HFD-induced obesity and related abnormalities through modulating gut microbiota, indicating that UAOS can act as potent prebiotic agents in treating obesity and related metabolic diseases.
32420551	8	46	theme	prebiotic	1461:1469	arg1	agents					1471:1476	potent prebiotic agents	1454:1476	potent prebiotic agents	1454:1476	These results suggest that UAOS can attenuate the HFD-induced obesity and related abnormalities through modulating gut microbiota, indicating that UAOS can act as potent prebiotic agents in treating obesity and related metabolic diseases.
32420551	1	47	theme	important	165:173	arg1	role					175:178	an important role	162:178	an important role	162:178	Gut microbiota plays an important role in the high-fat diet (HFD)-induced obesity and related metabolic syndrome (MetS).
32420551	8	48	theme	related	1365:1371	arg1	abnormalities					1373:1385	related abnormalities	1365:1385	related abnormalities	1365:1385	These results suggest that UAOS can attenuate the HFD-induced obesity and related abnormalities through modulating gut microbiota, indicating that UAOS can act as potent prebiotic agents in treating obesity and related metabolic diseases.
32420551	0	49	theme	gut	103:105	arg1	microbiota					107:116	gut microbiota	103:116	gut microbiota in high-fat-diet mice	103:138	Unsaturated alginate oligosaccharides attenuated obesity-related metabolic abnormalities by modulating gut microbiota in high-fat-diet mice.
32420551	2	50	theme	significant	383:393	arg1	effects					408:414	significant anti-obesity effects	383:414	significant anti-obesity effects	383:414	Our previous study has demonstrated that unsaturated alginate oligosaccharides (UAOS) degraded by alginate lyase possess significant anti-obesity effects in HFD-fed mice.
32420551	5	51	theme	overall	852:858	arg1	composition					860:870	the overall composition	848:870	the overall composition of the gut microbiota, which is highly associated with metabolic parameters	848:946	Subsequently, the microbiological analysis has revealed that UAOS treatment can modulate the overall composition of the gut microbiota, which is highly associated with metabolic parameters.
32420551	3	52	theme	insulin	572:578	arg1	resistance					580:589	insulin resistance	572:589	insulin resistance	572:589	Herein, we further established that UAOS could significantly ameliorate obesity-related metabolic abnormalities, including hyperlipidemia, insulin resistance and low-grade inflammation.
32420551	4	53	theme	metabolic	672:680	arg1	abnormalities					682:694	these metabolic abnormalities	666:694	these metabolic abnormalities	666:694	Particularly, the beneficial effect of UAOS on these metabolic abnormalities could be significantly reversed by antibiotic supplementation.
34409950	0	0	theme	Saccharification	78:93	arg1	Potential					95:103	Its Saccharification Potential	74:103	Its Saccharification Potential for Agricultural Straws	74:127	Characterization of a GH8 β-1,4-Glucanase from Bacillus subtilis B111 and Its Saccharification Potential for Agricultural Straws.
34409950	2	1	theme	%	342:342	arg1	identity					344:351	76.8% identity	338:351	76.8% identity	338:351	The recombinant celA1805 contains a glycosyl hydrolase (GH) family 8 domain and shared 76.8% identity with endo-1,4-β-glucanase from Bacillus sp.
34409950	4	2	from	temperature	534:544	arg1	stable					513:518	stable	513:518	stable	513:518	Results showed that the optimal pH and temperature of celA1805 were 6.0 and 50°C, respectively, and it was stable at pH 3-9 and temperature ≤50°C. Metal ions slightly affected enzyme activity, but chemical agents generally inhibited enzyme activity.
34409950	9	3	theme	potential	1320:1328	arg1	applications					1330:1341	potential applications	1320:1341	potential applications of celA1805 in biomass saccharification	1320:1381	The results obtained in this study suggest potential applications of celA1805 in biomass saccharification.
34409950	2	4	theme	76.8	338:341	arg1	%					342:342	%	342:342	%	342:342	The recombinant celA1805 contains a glycosyl hydrolase (GH) family 8 domain and shared 76.8% identity with endo-1,4-β-glucanase from Bacillus sp.
34409950	9	5	theme	biomass	1358:1364	arg1	saccharification					1366:1381	biomass saccharification	1358:1381	biomass saccharification	1358:1381	The results obtained in this study suggest potential applications of celA1805 in biomass saccharification.
34409950	0	6	from	Characterization	0:15	arg1	B111					65:68	Bacillus subtilis B111	47:68	Bacillus subtilis B111	47:68	Characterization of a GH8 β-1,4-Glucanase from Bacillus subtilis B111 and Its Saccharification Potential for Agricultural Straws.
34409950	2	7	theme	hydrolase	296:304	arg1	domain					320:325	glycosyl hydrolase (GH) family 8 domain	287:325	a glycosyl hydrolase (GH) family 8 domain	285:325	The recombinant celA1805 contains a glycosyl hydrolase (GH) family 8 domain and shared 76.8% identity with endo-1,4-β-glucanase from Bacillus sp.
34409950	0	8	theme	Potential	95:103	arg1	Characterization					0:15	Characterization	0:15	Characterization of a GH8 β-1,4-Glucanase from Bacillus subtilis B111 and Its Saccharification Potential for Agricultural Straws.	0:128	Characterization of a GH8 β-1,4-Glucanase from Bacillus subtilis B111 and Its Saccharification Potential for Agricultural Straws.
34409950	2	9	theme	glycosyl	287:294	arg1	domain					320:325	glycosyl hydrolase (GH) family 8 domain	287:325	a glycosyl hydrolase (GH) family 8 domain	285:325	The recombinant celA1805 contains a glycosyl hydrolase (GH) family 8 domain and shared 76.8% identity with endo-1,4-β-glucanase from Bacillus sp.
34409950	4	10	theme	Metal	553:557	arg1	ions					559:562	Metal ions	553:562	Metal ions	553:562	Results showed that the optimal pH and temperature of celA1805 were 6.0 and 50°C, respectively, and it was stable at pH 3-9 and temperature ≤50°C. Metal ions slightly affected enzyme activity, but chemical agents generally inhibited enzyme activity.
34409950	8	11	theme	sugars	1102:1107	arg1	concentrations					1075:1088	The concentrations	1071:1088	The concentrations of reducing sugars saccharified by celA1805 from wheat straw, rape straw, rice straw, peanut straw, and corn straw	1071:1203	The concentrations of reducing sugars saccharified by celA1805 from wheat straw, rape straw, rice straw, peanut straw, and corn straw were increased by 0.21, 0.51, 0.26, 0.36, and 0.66 mg/ml, respectively.
34409950	4	12	theme	celA1805	460:467	arg1	temperature					445:455	temperature	445:455	temperature	445:455	Results showed that the optimal pH and temperature of celA1805 were 6.0 and 50°C, respectively, and it was stable at pH 3-9 and temperature ≤50°C. Metal ions slightly affected enzyme activity, but chemical agents generally inhibited enzyme activity.
34409950	4	12	theme	celA1805	460:467	arg1	pH					438:439	optimal pH	430:439	optimal pH	430:439	Results showed that the optimal pH and temperature of celA1805 were 6.0 and 50°C, respectively, and it was stable at pH 3-9 and temperature ≤50°C. Metal ions slightly affected enzyme activity, but chemical agents generally inhibited enzyme activity.
34409950	1	13	theme	endo-β-1,4-glucanase	165:184	arg1	celA1805					192:199	celA1805	192:199	celA1805	192:199	Herein, we cloned and expressed an endo-β-1,4-glucanase gene (celA1805) from Bacillus subtilis B111 in Escherichia coli.
34409950	1	13	theme	endo-β-1,4-glucanase	165:184	arg1	gene					186:189	an endo-β-1,4-glucanase gene	162:189	an endo-β-1,4-glucanase gene (celA1805) from Bacillus subtilis B111	162:228	Herein, we cloned and expressed an endo-β-1,4-glucanase gene (celA1805) from Bacillus subtilis B111 in Escherichia coli.
34409950	0	14	theme	Agricultural	109:120	arg1	Straws					122:127	Agricultural Straws	109:127	Agricultural Straws	109:127	Characterization of a GH8 β-1,4-Glucanase from Bacillus subtilis B111 and Its Saccharification Potential for Agricultural Straws.
34409950	2	15	theme	Bacillus	384:391	arg1	sp					393:394	Bacillus sp	384:394	Bacillus sp	384:394	The recombinant celA1805 contains a glycosyl hydrolase (GH) family 8 domain and shared 76.8% identity with endo-1,4-β-glucanase from Bacillus sp.
34409950	2	16	theme	family	311:316	arg1	domain					320:325	glycosyl hydrolase (GH) family 8 domain	287:325	a glycosyl hydrolase (GH) family 8 domain	285:325	The recombinant celA1805 contains a glycosyl hydrolase (GH) family 8 domain and shared 76.8% identity with endo-1,4-β-glucanase from Bacillus sp.
34409950	2	17	contain	contains	276:283	arg2	domain					320:325	glycosyl hydrolase (GH) family 8 domain	287:325	a glycosyl hydrolase (GH) family 8 domain	285:325	The recombinant celA1805 contains a glycosyl hydrolase (GH) family 8 domain and shared 76.8% identity with endo-1,4-β-glucanase from Bacillus sp.
34409950	2	17	contain	contains	276:283	arg1	celA1805					267:274	The recombinant celA1805	251:274	The recombinant celA1805	251:274	The recombinant celA1805 contains a glycosyl hydrolase (GH) family 8 domain and shared 76.8% identity with endo-1,4-β-glucanase from Bacillus sp.
34409950	4	18	theme	enzyme	639:644	arg1	activity					646:653	enzyme activity	639:653	enzyme activity	639:653	Results showed that the optimal pH and temperature of celA1805 were 6.0 and 50°C, respectively, and it was stable at pH 3-9 and temperature ≤50°C. Metal ions slightly affected enzyme activity, but chemical agents generally inhibited enzyme activity.
34409950	0	19	theme	GH8	22:24	arg1	β-1,4-Glucanase					26:40	a GH8 β-1,4-Glucanase	20:40	a GH8 β-1,4-Glucanase from Bacillus subtilis B111	20:68	Characterization of a GH8 β-1,4-Glucanase from Bacillus subtilis B111 and Its Saccharification Potential for Agricultural Straws.
34409950	8	20	theme	reducing	1093:1100	arg1	sugars					1102:1107	reducing sugars	1093:1107	reducing sugars saccharified by celA1805 from wheat straw, rape straw, rice straw, peanut straw, and corn straw	1093:1203	The concentrations of reducing sugars saccharified by celA1805 from wheat straw, rape straw, rice straw, peanut straw, and corn straw were increased by 0.21, 0.51, 0.26, 0.36, and 0.66 mg/ml, respectively.
34409950	2	21	theme	GH	307:308	arg1	domain					320:325	glycosyl hydrolase (GH) family 8 domain	287:325	a glycosyl hydrolase (GH) family 8 domain	285:325	The recombinant celA1805 contains a glycosyl hydrolase (GH) family 8 domain and shared 76.8% identity with endo-1,4-β-glucanase from Bacillus sp.
34409950	8	22	theme	peanut	1176:1181	arg1	straw					1183:1187	peanut straw	1176:1187	peanut straw	1176:1187	The concentrations of reducing sugars saccharified by celA1805 from wheat straw, rape straw, rice straw, peanut straw, and corn straw were increased by 0.21, 0.51, 0.26, 0.36, and 0.66 mg/ml, respectively.
34409950	4	23	dep	pH	438:439	arg1	the					426:428	the	426:428	the	426:428	Results showed that the optimal pH and temperature of celA1805 were 6.0 and 50°C, respectively, and it was stable at pH 3-9 and temperature ≤50°C. Metal ions slightly affected enzyme activity, but chemical agents generally inhibited enzyme activity.
34409950	9	24	from	applications	1330:1341	arg1	saccharification					1366:1381	biomass saccharification	1358:1381	biomass saccharification	1358:1381	The results obtained in this study suggest potential applications of celA1805 in biomass saccharification.
34409950	5	25	theme	wide	684:687	arg1	specificity					699:709	a wide substrate specificity	682:709	a wide substrate specificity to CMC, barley β-glucan, lichenin, chitosan, PASC and avicel	682:770	Moreover, celA1805 showed a wide substrate specificity to CMC, barley β-glucan, lichenin, chitosan, PASC and avicel.
34409950	9	26	theme	celA1805	1346:1353	arg1	applications					1330:1341	potential applications	1320:1341	potential applications of celA1805 in biomass saccharification	1320:1381	The results obtained in this study suggest potential applications of celA1805 in biomass saccharification.
34409950	8	27	theme	rice	1164:1167	arg1	straw					1169:1173	rice straw	1164:1173	rice straw	1164:1173	The concentrations of reducing sugars saccharified by celA1805 from wheat straw, rape straw, rice straw, peanut straw, and corn straw were increased by 0.21, 0.51, 0.26, 0.36, and 0.66 mg/ml, respectively.
34409950	1	28	theme	Bacillus	207:214	arg1	B111					225:228	Bacillus subtilis B111	207:228	Bacillus subtilis B111	207:228	Herein, we cloned and expressed an endo-β-1,4-glucanase gene (celA1805) from Bacillus subtilis B111 in Escherichia coli.
34409950	0	29	theme	β-1,4-Glucanase	26:40	arg1	Characterization					0:15	Characterization	0:15	Characterization of a GH8 β-1,4-Glucanase from Bacillus subtilis B111 and Its Saccharification Potential for Agricultural Straws.	0:128	Characterization of a GH8 β-1,4-Glucanase from Bacillus subtilis B111 and Its Saccharification Potential for Agricultural Straws.
34409950	5	30	theme	substrate	689:697	arg1	specificity					699:709	a wide substrate specificity	682:709	a wide substrate specificity to CMC, barley β-glucan, lichenin, chitosan, PASC and avicel	682:770	Moreover, celA1805 showed a wide substrate specificity to CMC, barley β-glucan, lichenin, chitosan, PASC and avicel.
34409950	6	31	theme	celA1805	799:806	arg1	mg/ml					818:822	1.78 mg/ml	813:822	1.78 mg/ml	813:822	The Km and Vmax values of celA1805 were 1.78 mg/ml and 50.09 μmol/min/mg.
34409950	6	31	theme	celA1805	799:806	arg1	values					789:794	The Km and Vmax values	773:794	The Km and Vmax values of celA1805	773:806	The Km and Vmax values of celA1805 were 1.78 mg/ml and 50.09 μmol/min/mg.
34409950	0	32	dep	Bacillus	47:54	arg1	subtilis					56:63	subtilis	56:63	subtilis	56:63	Characterization of a GH8 β-1,4-Glucanase from Bacillus subtilis B111 and Its Saccharification Potential for Agricultural Straws.
34409950	4	33	theme	chemical	603:610	arg1	agents					612:617	chemical agents	603:617	chemical agents	603:617	Results showed that the optimal pH and temperature of celA1805 were 6.0 and 50°C, respectively, and it was stable at pH 3-9 and temperature ≤50°C. Metal ions slightly affected enzyme activity, but chemical agents generally inhibited enzyme activity.
34409950	8	34	theme	rape	1152:1155	arg1	straw					1157:1161	rape straw	1152:1161	rape straw	1152:1161	The concentrations of reducing sugars saccharified by celA1805 from wheat straw, rape straw, rice straw, peanut straw, and corn straw were increased by 0.21, 0.51, 0.26, 0.36, and 0.66 mg/ml, respectively.
34409950	8	35	theme	wheat	1139:1143	arg1	straw					1145:1149	wheat straw	1139:1149	wheat straw	1139:1149	The concentrations of reducing sugars saccharified by celA1805 from wheat straw, rape straw, rice straw, peanut straw, and corn straw were increased by 0.21, 0.51, 0.26, 0.36, and 0.66 mg/ml, respectively.
34409950	0	36	from	B111	65:68	arg1	Characterization					0:15	Characterization	0:15	Characterization of a GH8 β-1,4-Glucanase from Bacillus subtilis B111 and Its Saccharification Potential for Agricultural Straws.	0:128	Characterization of a GH8 β-1,4-Glucanase from Bacillus subtilis B111 and Its Saccharification Potential for Agricultural Straws.
34409950	0	36	from	B111	65:68	arg1	Potential					95:103	Its Saccharification Potential	74:103	Its Saccharification Potential for Agricultural Straws	74:127	Characterization of a GH8 β-1,4-Glucanase from Bacillus subtilis B111 and Its Saccharification Potential for Agricultural Straws.
34409950	0	36	from	B111	65:68	arg1	β-1,4-Glucanase					26:40	a GH8 β-1,4-Glucanase	20:40	a GH8 β-1,4-Glucanase from Bacillus subtilis B111	20:68	Characterization of a GH8 β-1,4-Glucanase from Bacillus subtilis B111 and Its Saccharification Potential for Agricultural Straws.
34409950	8	37	theme	corn	1194:1197	arg1	straw					1199:1203	corn straw	1194:1203	corn straw	1194:1203	The concentrations of reducing sugars saccharified by celA1805 from wheat straw, rape straw, rice straw, peanut straw, and corn straw were increased by 0.21, 0.51, 0.26, 0.36, and 0.66 mg/ml, respectively.
34409950	0	38	theme	Bacillus	47:54	arg1	B111					65:68	Bacillus subtilis B111	47:68	Bacillus subtilis B111	47:68	Characterization of a GH8 β-1,4-Glucanase from Bacillus subtilis B111 and Its Saccharification Potential for Agricultural Straws.
34409950	2	39	from	sp	393:394	arg1	endo-1,4-β-glucanase					358:377	endo-1,4-β-glucanase	358:377	endo-1,4-β-glucanase from Bacillus sp	358:394	The recombinant celA1805 contains a glycosyl hydrolase (GH) family 8 domain and shared 76.8% identity with endo-1,4-β-glucanase from Bacillus sp.
34409950	1	40	from	B111	225:228	arg1	celA1805					192:199	celA1805	192:199	celA1805	192:199	Herein, we cloned and expressed an endo-β-1,4-glucanase gene (celA1805) from Bacillus subtilis B111 in Escherichia coli.
34409950	1	40	from	B111	225:228	arg1	gene					186:189	an endo-β-1,4-glucanase gene	162:189	an endo-β-1,4-glucanase gene (celA1805) from Bacillus subtilis B111	162:228	Herein, we cloned and expressed an endo-β-1,4-glucanase gene (celA1805) from Bacillus subtilis B111 in Escherichia coli.
34409950	6	41	theme	Vmax	784:787	arg1	mg/ml					818:822	1.78 mg/ml	813:822	1.78 mg/ml	813:822	The Km and Vmax values of celA1805 were 1.78 mg/ml and 50.09 μmol/min/mg.
34409950	6	41	theme	Vmax	784:787	arg1	values					789:794	The Km and Vmax values	773:794	The Km and Vmax values of celA1805	773:806	The Km and Vmax values of celA1805 were 1.78 mg/ml and 50.09 μmol/min/mg.
34409950	1	42	dep	Bacillus	207:214	arg1	subtilis					216:223	subtilis	216:223	subtilis	216:223	Herein, we cloned and expressed an endo-β-1,4-glucanase gene (celA1805) from Bacillus subtilis B111 in Escherichia coli.
34409950	4	43	theme	optimal	430:436	arg1	pH					438:439	optimal pH	430:439	optimal pH	430:439	Results showed that the optimal pH and temperature of celA1805 were 6.0 and 50°C, respectively, and it was stable at pH 3-9 and temperature ≤50°C. Metal ions slightly affected enzyme activity, but chemical agents generally inhibited enzyme activity.
34409950	2	44	theme	recombinant	255:265	arg1	celA1805					267:274	The recombinant celA1805	251:274	The recombinant celA1805	251:274	The recombinant celA1805 contains a glycosyl hydrolase (GH) family 8 domain and shared 76.8% identity with endo-1,4-β-glucanase from Bacillus sp.
34409950	5	45	theme	barley	719:724	arg1	β-glucan					726:733	barley β-glucan	719:733	barley β-glucan	719:733	Moreover, celA1805 showed a wide substrate specificity to CMC, barley β-glucan, lichenin, chitosan, PASC and avicel.
34409950	6	46	theme	Km	777:778	arg1	mg/ml					818:822	1.78 mg/ml	813:822	1.78 mg/ml	813:822	The Km and Vmax values of celA1805 were 1.78 mg/ml and 50.09 μmol/min/mg.
34409950	6	46	theme	Km	777:778	arg1	values					789:794	The Km and Vmax values	773:794	The Km and Vmax values of celA1805	773:806	The Km and Vmax values of celA1805 were 1.78 mg/ml and 50.09 μmol/min/mg.
34409950	4	47	from	pH	523:524	arg1	stable					513:518	stable	513:518	stable	513:518	Results showed that the optimal pH and temperature of celA1805 were 6.0 and 50°C, respectively, and it was stable at pH 3-9 and temperature ≤50°C. Metal ions slightly affected enzyme activity, but chemical agents generally inhibited enzyme activity.
34409950	4	48	theme	enzyme	582:587	arg1	activity					589:596	enzyme activity	582:596	enzyme activity	582:596	Results showed that the optimal pH and temperature of celA1805 were 6.0 and 50°C, respectively, and it was stable at pH 3-9 and temperature ≤50°C. Metal ions slightly affected enzyme activity, but chemical agents generally inhibited enzyme activity.
34474320	14	0	theme	collagen	2916:2923	arg1	network					2925:2931	the collagen network	2912:2931	the collagen network	2912:2931	This, in turn, helps protect the solid matrix against large distortions and strains at the subchondral junction, pointing to the importance of the collagen network in deep zone in assisting high-load-bearing cartilage tissues.
34474320	10	1	theme	PLM	2112:2114	arg1	images					2116:2121	PLM images	2112:2121	PLM images	2112:2121	PLM images revealed a sizable deep zone in the kangaroo knee cartilage where collagen fibrils were oriented perpendicular to the subchondral bone.
34474320	4	2	from	study	720:724	arg1	cartilage					768:776	the low-load-bearing kangaroo shoulder cartilage	729:776	the low-load-bearing kangaroo shoulder cartilage	729:776	Further, in contrast to the reported findings on high-load bearing knee cartilage, our previous study on the low-load-bearing kangaroo shoulder cartilage indicated that proteoglycan and collagen contribution remained steady as the strain-rate increases.
34474320	12	3	located	observed	2399:2406	arg2	differences					2387:2397	the biomechanical differences	2369:2397	the biomechanical differences observed in the knee and shoulder cartilage	2369:2441	FE model confirmed that the biomechanical differences observed in the knee and shoulder cartilage are due to the differences in the collagen fibril arrangement in the deep zone.
34474320	12	3	located	observed	2399:2406	arg1	shoulder					2424:2431	shoulder	2424:2431	shoulder	2424:2431	FE model confirmed that the biomechanical differences observed in the knee and shoulder cartilage are due to the differences in the collagen fibril arrangement in the deep zone.
34474320	12	3	located	observed	2399:2406	arg1	knee					2415:2418	knee	2415:2418	knee	2415:2418	FE model confirmed that the biomechanical differences observed in the knee and shoulder cartilage are due to the differences in the collagen fibril arrangement in the deep zone.
34474320	12	3	located	observed	2399:2406	arg2	due					2447:2449	due	2447:2449	due	2447:2449	FE model confirmed that the biomechanical differences observed in the knee and shoulder cartilage are due to the differences in the collagen fibril arrangement in the deep zone.
34474320	11	4	theme	deep	2295:2298	arg1	zone					2300:2303	no such apparent deep zone	2278:2303	no such apparent deep zone	2278:2303	On the other hand, no such apparent deep zone was observed in the shoulder cartilage.
34474320	14	5	theme	large	2823:2827	arg1	distortions					2829:2839	large distortions	2823:2839	large distortions	2823:2839	This, in turn, helps protect the solid matrix against large distortions and strains at the subchondral junction, pointing to the importance of the collagen network in deep zone in assisting high-load-bearing cartilage tissues.
34474320	13	6	theme	high-load-bearing	2571:2587	arg1	tissues					2599:2605	high-load-bearing cartilage tissues	2571:2605	high-load-bearing cartilage tissues	2571:2605	From these results, it can be concluded that in high-load-bearing cartilage tissues, the collagen network in the deep zone assists in increasing the stiffness of tissue with strain-rate and plays a significant role in supporting transient loads.
34474320	13	7	theme	significant	2721:2731	arg1	role					2733:2736	a significant role	2719:2736	a significant role	2719:2736	From these results, it can be concluded that in high-load-bearing cartilage tissues, the collagen network in the deep zone assists in increasing the stiffness of tissue with strain-rate and plays a significant role in supporting transient loads.
34474320	2	8	theme	other	304:308	arg1	hand					310:313	the other hand	300:313	the other hand	300:313	On the other hand, the contribution from the collagen network has been suggested to increase as the strain-rate increases.
34474320	12	9	dep	knee	2415:2418	arg1	the					2411:2413	the	2411:2413	the	2411:2413	FE model confirmed that the biomechanical differences observed in the knee and shoulder cartilage are due to the differences in the collagen fibril arrangement in the deep zone.
34474320	12	9	dep	knee	2415:2418	arg1	cartilage					2433:2441	cartilage	2433:2441	cartilage	2433:2441	FE model confirmed that the biomechanical differences observed in the knee and shoulder cartilage are due to the differences in the collagen fibril arrangement in the deep zone.
34474320	11	10	theme	shoulder	2325:2332	arg1	cartilage					2334:2342	the shoulder cartilage	2321:2342	the shoulder cartilage	2321:2342	On the other hand, no such apparent deep zone was observed in the shoulder cartilage.
34474320	2	11	from	network	351:357	arg1	contribution					320:331	the contribution	316:331	the contribution from the collagen network	316:357	On the other hand, the contribution from the collagen network has been suggested to increase as the strain-rate increases.
34474320	10	12	theme	sizable	2134:2140	arg1	zone					2147:2150	a sizable deep zone	2132:2150	a sizable deep zone in the kangaroo knee cartilage where collagen fibrils were oriented perpendicular to the subchondral bone	2132:2256	PLM images revealed a sizable deep zone in the kangaroo knee cartilage where collagen fibrils were oriented perpendicular to the subchondral bone.
34474320	13	13	theme	tissue	2685:2690	arg1	stiffness					2672:2680	the stiffness	2668:2680	the stiffness of tissue	2668:2690	From these results, it can be concluded that in high-load-bearing cartilage tissues, the collagen network in the deep zone assists in increasing the stiffness of tissue with strain-rate and plays a significant role in supporting transient loads.
34474320	8	14	with	model	1748:1752	arg1	objective					1763:1771	the objective	1759:1771	the objective of explaining the mechanisms underlying differences observed between the two tissues	1759:1856	Identified differences in the collagen architecture and proteoglycan composition were incorporated in a fibril-reinforced porohyperelastic Finite Element (FE) model with the objective of explaining the mechanisms underlying differences observed between the two tissues.
34474320	7	15	theme	histological	1566:1577	arg1	staining					1579:1586	histological staining	1566:1586	histological staining	1566:1586	Then, structural and compositional differences between the kangaroo knee and shoulder cartilage were assessed qualitatively through polarised light microscopy (PLM) imaging and histological staining.
34474320	14	16	theme	deep	2936:2939	arg1	zone					2941:2944	deep zone	2936:2944	deep zone in assisting high-load-bearing cartilage tissues	2936:2993	This, in turn, helps protect the solid matrix against large distortions and strains at the subchondral junction, pointing to the importance of the collagen network in deep zone in assisting high-load-bearing cartilage tissues.
34474320	6	17	theme	network	1242:1248	arg1	contribution					1199:1210	the contribution	1195:1210	the contribution of proteoglycans and collagen network	1195:1248	Firstly, in order to quantify the contribution of proteoglycans and collagen network, the indentation testings on normal, proteoglycan, and collagen-degraded kangaroo knee cartilage were conducted at different strain-rates.
34474320	13	18	theme	deep	2636:2639	arg1	zone					2641:2644	the deep zone	2632:2644	the deep zone	2632:2644	From these results, it can be concluded that in high-load-bearing cartilage tissues, the collagen network in the deep zone assists in increasing the stiffness of tissue with strain-rate and plays a significant role in supporting transient loads.
34474320	10	19	theme	knee	2168:2171	arg1	cartilage					2173:2181	the kangaroo knee cartilage	2155:2181	the kangaroo knee cartilage where collagen fibrils were oriented perpendicular to the subchondral bone	2155:2256	PLM images revealed a sizable deep zone in the kangaroo knee cartilage where collagen fibrils were oriented perpendicular to the subchondral bone.
34474320	6	20	theme	knee	1332:1335	arg1	cartilage					1337:1345	normal, proteoglycan, and collagen-degraded kangaroo knee cartilage	1279:1345	normal, proteoglycan, and collagen-degraded kangaroo knee cartilage	1279:1345	Firstly, in order to quantify the contribution of proteoglycans and collagen network, the indentation testings on normal, proteoglycan, and collagen-degraded kangaroo knee cartilage were conducted at different strain-rates.
34474320	1	21	theme	tissues	221:227	arg1	behaviour					198:206	the strain-rate-dependent mechanical behaviour	161:206	the strain-rate-dependent mechanical behaviour of cartilage tissues	161:227	The contribution of the proteoglycan to the strain-rate-dependent mechanical behaviour of cartilage tissues has been suggested to decrease with an increase in the strain-rate.
34474320	14	22	theme	solid	2802:2806	arg1	matrix					2808:2813	the solid matrix	2798:2813	the solid matrix	2798:2813	This, in turn, helps protect the solid matrix against large distortions and strains at the subchondral junction, pointing to the importance of the collagen network in deep zone in assisting high-load-bearing cartilage tissues.
34474320	9	23	theme	Experimental	1859:1870	arg1	results					1872:1878	Experimental results	1859:1878	Experimental results on knee cartilage	1859:1896	Experimental results on knee cartilage indicated that when the strain-rate increases, proteoglycan contribution decreases while collagen contribution increases, where statistically significant differences were identified at each strain-rate (p < 0.05).
34474320	13	24	theme	cartilage	2589:2597	arg1	tissues					2599:2605	high-load-bearing cartilage tissues	2571:2605	high-load-bearing cartilage tissues	2571:2605	From these results, it can be concluded that in high-load-bearing cartilage tissues, the collagen network in the deep zone assists in increasing the stiffness of tissue with strain-rate and plays a significant role in supporting transient loads.
34474320	5	25	theme	shoulder	1145:1152	arg1	cartilage					1154:1162	the kangaroo shoulder cartilage	1132:1162	the kangaroo shoulder cartilage	1132:1162	Therefore, in the present study, we experimentally investigate the contribution of proteoglycan and collagen network to the strain-rate-dependent behaviour of the kangaroo knee cartilage, and plausible reasons for the differences observed in relation to the kangaroo shoulder cartilage.
34474320	5	26	theme	present	896:902	arg1	study					904:908	the present study	892:908	the present study	892:908	Therefore, in the present study, we experimentally investigate the contribution of proteoglycan and collagen network to the strain-rate-dependent behaviour of the kangaroo knee cartilage, and plausible reasons for the differences observed in relation to the kangaroo shoulder cartilage.
34474320	5	27	theme	network	987:993	arg1	reasons					1080:1086	plausible reasons	1070:1086	plausible reasons for the differences observed in relation to the kangaroo shoulder cartilage	1070:1162	Therefore, in the present study, we experimentally investigate the contribution of proteoglycan and collagen network to the strain-rate-dependent behaviour of the kangaroo knee cartilage, and plausible reasons for the differences observed in relation to the kangaroo shoulder cartilage.
34474320	5	27	theme	network	987:993	arg1	contribution					945:956	the contribution	941:956	the contribution of proteoglycan and collagen network to the strain-rate-dependent behaviour of the kangaroo knee cartilage	941:1063	Therefore, in the present study, we experimentally investigate the contribution of proteoglycan and collagen network to the strain-rate-dependent behaviour of the kangaroo knee cartilage, and plausible reasons for the differences observed in relation to the kangaroo shoulder cartilage.
34474320	14	28	theme	high-load-bearing	2959:2975	arg1	tissues					2987:2993	high-load-bearing cartilage tissues	2959:2993	high-load-bearing cartilage tissues	2959:2993	This, in turn, helps protect the solid matrix against large distortions and strains at the subchondral junction, pointing to the importance of the collagen network in deep zone in assisting high-load-bearing cartilage tissues.
34474320	10	29	from	zone	2147:2150	arg1	cartilage					2173:2181	the kangaroo knee cartilage	2155:2181	the kangaroo knee cartilage where collagen fibrils were oriented perpendicular to the subchondral bone	2155:2256	PLM images revealed a sizable deep zone in the kangaroo knee cartilage where collagen fibrils were oriented perpendicular to the subchondral bone.
34474320	11	30	theme	such	2281:2284	arg1	zone					2300:2303	no such apparent deep zone	2278:2303	no such apparent deep zone	2278:2303	On the other hand, no such apparent deep zone was observed in the shoulder cartilage.
34474320	1	31	from	increase	268:275	arg1	strain-rate					284:294	the strain-rate	280:294	the strain-rate	280:294	The contribution of the proteoglycan to the strain-rate-dependent mechanical behaviour of cartilage tissues has been suggested to decrease with an increase in the strain-rate.
34474320	4	32	dep	proteoglycan	793:804	arg1	contribution					819:830	contribution	819:830	contribution	819:830	Further, in contrast to the reported findings on high-load bearing knee cartilage, our previous study on the low-load-bearing kangaroo shoulder cartilage indicated that proteoglycan and collagen contribution remained steady as the strain-rate increases.
34474320	8	33	theme	Identified	1589:1598	arg1	differences					1600:1610	Identified differences	1589:1610	Identified differences in the collagen architecture and proteoglycan composition	1589:1668	Identified differences in the collagen architecture and proteoglycan composition were incorporated in a fibril-reinforced porohyperelastic Finite Element (FE) model with the objective of explaining the mechanisms underlying differences observed between the two tissues.
34474320	6	34	theme	proteoglycan	1287:1298	arg1	cartilage					1337:1345	normal, proteoglycan, and collagen-degraded kangaroo knee cartilage	1279:1345	normal, proteoglycan, and collagen-degraded kangaroo knee cartilage	1279:1345	Firstly, in order to quantify the contribution of proteoglycans and collagen network, the indentation testings on normal, proteoglycan, and collagen-degraded kangaroo knee cartilage were conducted at different strain-rates.
34474320	1	35	theme	proteoglycan	145:156	arg1	contribution					125:136	The contribution	121:136	The contribution of the proteoglycan to the strain-rate-dependent mechanical behaviour of cartilage tissues	121:227	The contribution of the proteoglycan to the strain-rate-dependent mechanical behaviour of cartilage tissues has been suggested to decrease with an increase in the strain-rate.
34474320	6	36	theme	normal	1279:1284	arg1	cartilage					1337:1345	normal, proteoglycan, and collagen-degraded kangaroo knee cartilage	1279:1345	normal, proteoglycan, and collagen-degraded kangaroo knee cartilage	1279:1345	Firstly, in order to quantify the contribution of proteoglycans and collagen network, the indentation testings on normal, proteoglycan, and collagen-degraded kangaroo knee cartilage were conducted at different strain-rates.
34474320	8	37	theme	collagen	1619:1626	arg1	architecture					1628:1639	collagen architecture	1619:1639	collagen architecture	1619:1639	Identified differences in the collagen architecture and proteoglycan composition were incorporated in a fibril-reinforced porohyperelastic Finite Element (FE) model with the objective of explaining the mechanisms underlying differences observed between the two tissues.
34474320	0	38	theme	knee	92:95	arg1	behaviour					79:87	the strain-rate-dependent mechanical behaviour	42:87	the strain-rate-dependent mechanical behaviour of knee and shoulder cartilage	42:118	Proteoglycan and collagen contribution to the strain-rate-dependent mechanical behaviour of knee and shoulder cartilage.
34474320	1	39	theme	mechanical	187:196	arg1	behaviour					198:206	the strain-rate-dependent mechanical behaviour	161:206	the strain-rate-dependent mechanical behaviour of cartilage tissues	161:227	The contribution of the proteoglycan to the strain-rate-dependent mechanical behaviour of cartilage tissues has been suggested to decrease with an increase in the strain-rate.
34474320	9	40	theme	proteoglycan	1945:1956	arg1	contribution					1958:1969	proteoglycan contribution	1945:1969	proteoglycan contribution	1945:1969	Experimental results on knee cartilage indicated that when the strain-rate increases, proteoglycan contribution decreases while collagen contribution increases, where statistically significant differences were identified at each strain-rate (p < 0.05).
34474320	12	41	theme	collagen	2477:2484	arg1	arrangement					2493:2503	the collagen fibril arrangement	2473:2503	the collagen fibril arrangement in the deep zone	2473:2520	FE model confirmed that the biomechanical differences observed in the knee and shoulder cartilage are due to the differences in the collagen fibril arrangement in the deep zone.
34474320	10	42	theme	subchondral	2241:2251	arg1	bone					2253:2256	the subchondral bone	2237:2256	the subchondral bone	2237:2256	PLM images revealed a sizable deep zone in the kangaroo knee cartilage where collagen fibrils were oriented perpendicular to the subchondral bone.
34474320	0	43	theme	shoulder	101:108	arg1	behaviour					79:87	the strain-rate-dependent mechanical behaviour	42:87	the strain-rate-dependent mechanical behaviour of knee and shoulder cartilage	42:118	Proteoglycan and collagen contribution to the strain-rate-dependent mechanical behaviour of knee and shoulder cartilage.
34474320	6	44	theme	indentation	1255:1265	arg1	testings					1267:1274	the indentation testings	1251:1274	the indentation testings on normal, proteoglycan, and collagen-degraded kangaroo knee cartilage	1251:1345	Firstly, in order to quantify the contribution of proteoglycans and collagen network, the indentation testings on normal, proteoglycan, and collagen-degraded kangaroo knee cartilage were conducted at different strain-rates.
34474320	5	45	theme	knee	1050:1053	arg1	cartilage					1055:1063	the kangaroo knee cartilage	1037:1063	the kangaroo knee cartilage	1037:1063	Therefore, in the present study, we experimentally investigate the contribution of proteoglycan and collagen network to the strain-rate-dependent behaviour of the kangaroo knee cartilage, and plausible reasons for the differences observed in relation to the kangaroo shoulder cartilage.
34474320	9	46	from	results	1872:1878	arg1	cartilage					1888:1896	knee cartilage	1883:1896	knee cartilage	1883:1896	Experimental results on knee cartilage indicated that when the strain-rate increases, proteoglycan contribution decreases while collagen contribution increases, where statistically significant differences were identified at each strain-rate (p < 0.05).
34474320	9	47	theme	collagen	1987:1994	arg1	contribution					1996:2007	collagen contribution	1987:2007	collagen contribution	1987:2007	Experimental results on knee cartilage indicated that when the strain-rate increases, proteoglycan contribution decreases while collagen contribution increases, where statistically significant differences were identified at each strain-rate (p < 0.05).
34474320	0	48	dep	knee	92:95	arg1	cartilage					110:118	cartilage	110:118	cartilage	110:118	Proteoglycan and collagen contribution to the strain-rate-dependent mechanical behaviour of knee and shoulder cartilage.
34474320	14	49	theme	network	2925:2931	arg1	importance					2898:2907	the importance	2894:2907	the importance of the collagen network in deep zone in assisting high-load-bearing cartilage tissues	2894:2993	This, in turn, helps protect the solid matrix against large distortions and strains at the subchondral junction, pointing to the importance of the collagen network in deep zone in assisting high-load-bearing cartilage tissues.
34474320	8	50	theme	porohyperelastic	1711:1726	arg1	model					1748:1752	a fibril-reinforced porohyperelastic Finite Element (FE) model	1691:1752	a fibril-reinforced porohyperelastic Finite Element (FE) model with the objective of explaining the mechanisms underlying differences observed between the two tissues	1691:1856	Identified differences in the collagen architecture and proteoglycan composition were incorporated in a fibril-reinforced porohyperelastic Finite Element (FE) model with the objective of explaining the mechanisms underlying differences observed between the two tissues.
34474320	3	51	theme	numerical	464:472	arg1	studies					474:480	numerical studies	464:480	numerical studies conducted on high-load-bearing knee cartilage tissues	464:534	These conclusions are drawn mainly based on numerical studies conducted on high-load-bearing knee cartilage tissues, while experimental evidence of these behaviours have not been demonstrated previously.
34474320	9	52	theme	significant	2040:2050	arg1	differences					2052:2062	statistically significant differences	2026:2062	statistically significant differences	2026:2062	Experimental results on knee cartilage indicated that when the strain-rate increases, proteoglycan contribution decreases while collagen contribution increases, where statistically significant differences were identified at each strain-rate (p < 0.05).
34474320	8	53	theme	Element	1735:1741	arg1	model					1748:1752	a fibril-reinforced porohyperelastic Finite Element (FE) model	1691:1752	a fibril-reinforced porohyperelastic Finite Element (FE) model with the objective of explaining the mechanisms underlying differences observed between the two tissues	1691:1856	Identified differences in the collagen architecture and proteoglycan composition were incorporated in a fibril-reinforced porohyperelastic Finite Element (FE) model with the objective of explaining the mechanisms underlying differences observed between the two tissues.
34474320	3	54	theme	cartilage	518:526	arg1	tissues					528:534	high-load-bearing knee cartilage tissues	495:534	high-load-bearing knee cartilage tissues	495:534	These conclusions are drawn mainly based on numerical studies conducted on high-load-bearing knee cartilage tissues, while experimental evidence of these behaviours have not been demonstrated previously.
34474320	0	55	theme	strain-rate-dependent	46:66	arg1	behaviour					79:87	the strain-rate-dependent mechanical behaviour	42:87	the strain-rate-dependent mechanical behaviour of knee and shoulder cartilage	42:118	Proteoglycan and collagen contribution to the strain-rate-dependent mechanical behaviour of knee and shoulder cartilage.
34474320	8	56	theme	FE	1744:1745	arg1	model					1748:1752	a fibril-reinforced porohyperelastic Finite Element (FE) model	1691:1752	a fibril-reinforced porohyperelastic Finite Element (FE) model with the objective of explaining the mechanisms underlying differences observed between the two tissues	1691:1856	Identified differences in the collagen architecture and proteoglycan composition were incorporated in a fibril-reinforced porohyperelastic Finite Element (FE) model with the objective of explaining the mechanisms underlying differences observed between the two tissues.
34474320	12	57	theme	FE	2345:2346	arg1	model					2348:2352	FE model	2345:2352	FE model	2345:2352	FE model confirmed that the biomechanical differences observed in the knee and shoulder cartilage are due to the differences in the collagen fibril arrangement in the deep zone.
34474320	2	58	theme	collagen	342:349	arg1	network					351:357	the collagen network	338:357	the collagen network	338:357	On the other hand, the contribution from the collagen network has been suggested to increase as the strain-rate increases.
34474320	5	59	theme	plausible	1070:1078	arg1	reasons					1080:1086	plausible reasons	1070:1086	plausible reasons for the differences observed in relation to the kangaroo shoulder cartilage	1070:1162	Therefore, in the present study, we experimentally investigate the contribution of proteoglycan and collagen network to the strain-rate-dependent behaviour of the kangaroo knee cartilage, and plausible reasons for the differences observed in relation to the kangaroo shoulder cartilage.
34474320	3	60	theme	experimental	543:554	arg1	evidence					556:563	experimental evidence	543:563	experimental evidence of these behaviours	543:583	These conclusions are drawn mainly based on numerical studies conducted on high-load-bearing knee cartilage tissues, while experimental evidence of these behaviours have not been demonstrated previously.
34474320	6	61	theme	different	1365:1373	arg1	strain-rates					1375:1386	different strain-rates	1365:1386	different strain-rates	1365:1386	Firstly, in order to quantify the contribution of proteoglycans and collagen network, the indentation testings on normal, proteoglycan, and collagen-degraded kangaroo knee cartilage were conducted at different strain-rates.
34474320	3	62	theme	behaviours	574:583	arg1	evidence					556:563	experimental evidence	543:563	experimental evidence of these behaviours	543:583	These conclusions are drawn mainly based on numerical studies conducted on high-load-bearing knee cartilage tissues, while experimental evidence of these behaviours have not been demonstrated previously.
34474320	8	63	dep	architecture	1628:1639	arg1	the					1615:1617	the	1615:1617	the	1615:1617	Identified differences in the collagen architecture and proteoglycan composition were incorporated in a fibril-reinforced porohyperelastic Finite Element (FE) model with the objective of explaining the mechanisms underlying differences observed between the two tissues.
34474320	7	64	theme	light	1531:1535	arg1	PLM					1549:1551	PLM	1549:1551	PLM	1549:1551	Then, structural and compositional differences between the kangaroo knee and shoulder cartilage were assessed qualitatively through polarised light microscopy (PLM) imaging and histological staining.
34474320	7	64	theme	light	1531:1535	arg1	microscopy					1537:1546	polarised light microscopy	1521:1546	polarised light microscopy (PLM) imaging	1521:1560	Then, structural and compositional differences between the kangaroo knee and shoulder cartilage were assessed qualitatively through polarised light microscopy (PLM) imaging and histological staining.
34474320	5	65	theme	proteoglycan	961:972	arg1	network					987:993	proteoglycan and collagen network	961:993	proteoglycan and collagen network	961:993	Therefore, in the present study, we experimentally investigate the contribution of proteoglycan and collagen network to the strain-rate-dependent behaviour of the kangaroo knee cartilage, and plausible reasons for the differences observed in relation to the kangaroo shoulder cartilage.
34474320	10	66	theme	deep	2142:2145	arg1	zone					2147:2150	a sizable deep zone	2132:2150	a sizable deep zone in the kangaroo knee cartilage where collagen fibrils were oriented perpendicular to the subchondral bone	2132:2256	PLM images revealed a sizable deep zone in the kangaroo knee cartilage where collagen fibrils were oriented perpendicular to the subchondral bone.
34474320	12	67	from	arrangement	2493:2503	arg1	zone					2517:2520	the deep zone	2508:2520	the deep zone	2508:2520	FE model confirmed that the biomechanical differences observed in the knee and shoulder cartilage are due to the differences in the collagen fibril arrangement in the deep zone.
34474320	4	68	theme	knee	691:694	arg1	cartilage					696:704	knee cartilage	691:704	knee cartilage	691:704	Further, in contrast to the reported findings on high-load bearing knee cartilage, our previous study on the low-load-bearing kangaroo shoulder cartilage indicated that proteoglycan and collagen contribution remained steady as the strain-rate increases.
34474320	14	69	theme	cartilage	2977:2985	arg1	tissues					2987:2993	high-load-bearing cartilage tissues	2959:2993	high-load-bearing cartilage tissues	2959:2993	This, in turn, helps protect the solid matrix against large distortions and strains at the subchondral junction, pointing to the importance of the collagen network in deep zone in assisting high-load-bearing cartilage tissues.
34474320	1	70	theme	cartilage	211:219	arg1	tissues					221:227	cartilage tissues	211:227	cartilage tissues	211:227	The contribution of the proteoglycan to the strain-rate-dependent mechanical behaviour of cartilage tissues has been suggested to decrease with an increase in the strain-rate.
34474320	12	71	theme	fibril	2486:2491	arg1	arrangement					2493:2503	the collagen fibril arrangement	2473:2503	the collagen fibril arrangement in the deep zone	2473:2520	FE model confirmed that the biomechanical differences observed in the knee and shoulder cartilage are due to the differences in the collagen fibril arrangement in the deep zone.
34474320	7	72	theme	microscopy	1537:1546	arg1	imaging					1554:1560	polarised light microscopy (PLM) imaging	1521:1560	polarised light microscopy (PLM) imaging	1521:1560	Then, structural and compositional differences between the kangaroo knee and shoulder cartilage were assessed qualitatively through polarised light microscopy (PLM) imaging and histological staining.
34474320	13	73	theme	transient	2752:2760	arg1	loads					2762:2766	transient loads	2752:2766	transient loads	2752:2766	From these results, it can be concluded that in high-load-bearing cartilage tissues, the collagen network in the deep zone assists in increasing the stiffness of tissue with strain-rate and plays a significant role in supporting transient loads.
34474320	6	74	theme	collagen	1233:1240	arg1	network					1242:1248	collagen network	1233:1248	collagen network	1233:1248	Firstly, in order to quantify the contribution of proteoglycans and collagen network, the indentation testings on normal, proteoglycan, and collagen-degraded kangaroo knee cartilage were conducted at different strain-rates.
34474320	7	75	theme	polarised	1521:1529	arg1	PLM					1549:1551	PLM	1549:1551	PLM	1549:1551	Then, structural and compositional differences between the kangaroo knee and shoulder cartilage were assessed qualitatively through polarised light microscopy (PLM) imaging and histological staining.
34474320	7	75	theme	polarised	1521:1529	arg1	microscopy					1537:1546	polarised light microscopy	1521:1546	polarised light microscopy (PLM) imaging	1521:1560	Then, structural and compositional differences between the kangaroo knee and shoulder cartilage were assessed qualitatively through polarised light microscopy (PLM) imaging and histological staining.
34474320	4	76	from	findings	661:668	arg1	high-load					673:681	high-load	673:681	high-load bearing knee cartilage	673:704	Further, in contrast to the reported findings on high-load bearing knee cartilage, our previous study on the low-load-bearing kangaroo shoulder cartilage indicated that proteoglycan and collagen contribution remained steady as the strain-rate increases.
34474320	9	77	theme	knee	1883:1886	arg1	cartilage					1888:1896	knee cartilage	1883:1896	knee cartilage	1883:1896	Experimental results on knee cartilage indicated that when the strain-rate increases, proteoglycan contribution decreases while collagen contribution increases, where statistically significant differences were identified at each strain-rate (p < 0.05).
34474320	6	78	theme	collagen-degraded	1305:1321	arg1	cartilage					1337:1345	normal, proteoglycan, and collagen-degraded kangaroo knee cartilage	1279:1345	normal, proteoglycan, and collagen-degraded kangaroo knee cartilage	1279:1345	Firstly, in order to quantify the contribution of proteoglycans and collagen network, the indentation testings on normal, proteoglycan, and collagen-degraded kangaroo knee cartilage were conducted at different strain-rates.
34474320	10	79	theme	collagen	2189:2196	arg1	fibrils					2198:2204	collagen fibrils	2189:2204	collagen fibrils	2189:2204	PLM images revealed a sizable deep zone in the kangaroo knee cartilage where collagen fibrils were oriented perpendicular to the subchondral bone.
34474320	5	80	theme	collagen	978:985	arg1	network					987:993	proteoglycan and collagen network	961:993	proteoglycan and collagen network	961:993	Therefore, in the present study, we experimentally investigate the contribution of proteoglycan and collagen network to the strain-rate-dependent behaviour of the kangaroo knee cartilage, and plausible reasons for the differences observed in relation to the kangaroo shoulder cartilage.
34474320	11	81	theme	apparent	2286:2293	arg1	zone					2300:2303	no such apparent deep zone	2278:2303	no such apparent deep zone	2278:2303	On the other hand, no such apparent deep zone was observed in the shoulder cartilage.
34474320	4	82	theme	low-load-bearing	733:748	arg1	cartilage					768:776	the low-load-bearing kangaroo shoulder cartilage	729:776	the low-load-bearing kangaroo shoulder cartilage	729:776	Further, in contrast to the reported findings on high-load bearing knee cartilage, our previous study on the low-load-bearing kangaroo shoulder cartilage indicated that proteoglycan and collagen contribution remained steady as the strain-rate increases.
34474320	12	83	theme	deep	2512:2515	arg1	zone					2517:2520	the deep zone	2508:2520	the deep zone	2508:2520	FE model confirmed that the biomechanical differences observed in the knee and shoulder cartilage are due to the differences in the collagen fibril arrangement in the deep zone.
34474320	7	84	theme	shoulder	1466:1473	arg1	cartilage					1475:1483	the kangaroo knee and shoulder cartilage	1444:1483	the kangaroo knee and shoulder cartilage	1444:1483	Then, structural and compositional differences between the kangaroo knee and shoulder cartilage were assessed qualitatively through polarised light microscopy (PLM) imaging and histological staining.
34474320	4	85	theme	shoulder	759:766	arg1	cartilage					768:776	the low-load-bearing kangaroo shoulder cartilage	729:776	the low-load-bearing kangaroo shoulder cartilage	729:776	Further, in contrast to the reported findings on high-load bearing knee cartilage, our previous study on the low-load-bearing kangaroo shoulder cartilage indicated that proteoglycan and collagen contribution remained steady as the strain-rate increases.
34474320	3	86	theme	high-load-bearing	495:511	arg1	tissues					528:534	high-load-bearing knee cartilage tissues	495:534	high-load-bearing knee cartilage tissues	495:534	These conclusions are drawn mainly based on numerical studies conducted on high-load-bearing knee cartilage tissues, while experimental evidence of these behaviours have not been demonstrated previously.
34474320	11	87	theme	other	2266:2270	arg1	hand					2272:2275	the other hand	2262:2275	the other hand	2262:2275	On the other hand, no such apparent deep zone was observed in the shoulder cartilage.
34474320	11	88	located	observed	2309:2316	arg1	hand					2272:2275	the other hand	2262:2275	the other hand	2262:2275	On the other hand, no such apparent deep zone was observed in the shoulder cartilage.
34474320	11	88	located	observed	2309:2316	arg2	zone					2300:2303	no such apparent deep zone	2278:2303	no such apparent deep zone	2278:2303	On the other hand, no such apparent deep zone was observed in the shoulder cartilage.
34474320	11	88	located	observed	2309:2316	arg1	cartilage					2334:2342	the shoulder cartilage	2321:2342	the shoulder cartilage	2321:2342	On the other hand, no such apparent deep zone was observed in the shoulder cartilage.
34474320	7	89	theme	knee	1457:1460	arg1	cartilage					1475:1483	the kangaroo knee and shoulder cartilage	1444:1483	the kangaroo knee and shoulder cartilage	1444:1483	Then, structural and compositional differences between the kangaroo knee and shoulder cartilage were assessed qualitatively through polarised light microscopy (PLM) imaging and histological staining.
34474320	4	90	theme	previous	711:718	arg1	study					720:724	our previous study	707:724	our previous study on the low-load-bearing kangaroo shoulder cartilage	707:776	Further, in contrast to the reported findings on high-load bearing knee cartilage, our previous study on the low-load-bearing kangaroo shoulder cartilage indicated that proteoglycan and collagen contribution remained steady as the strain-rate increases.
34474320	13	91	from	network	2621:2627	arg1	zone					2641:2644	the deep zone	2632:2644	the deep zone	2632:2644	From these results, it can be concluded that in high-load-bearing cartilage tissues, the collagen network in the deep zone assists in increasing the stiffness of tissue with strain-rate and plays a significant role in supporting transient loads.
34474320	1	92	theme	strain-rate-dependent	165:185	arg1	behaviour					198:206	the strain-rate-dependent mechanical behaviour	161:206	the strain-rate-dependent mechanical behaviour of cartilage tissues	161:227	The contribution of the proteoglycan to the strain-rate-dependent mechanical behaviour of cartilage tissues has been suggested to decrease with an increase in the strain-rate.
34474320	8	93	theme	proteoglycan	1645:1656	arg1	composition					1658:1668	proteoglycan composition	1645:1668	proteoglycan composition	1645:1668	Identified differences in the collagen architecture and proteoglycan composition were incorporated in a fibril-reinforced porohyperelastic Finite Element (FE) model with the objective of explaining the mechanisms underlying differences observed between the two tissues.
34474320	12	94	from	differences	2458:2468	arg1	arrangement					2493:2503	the collagen fibril arrangement	2473:2503	the collagen fibril arrangement in the deep zone	2473:2520	FE model confirmed that the biomechanical differences observed in the knee and shoulder cartilage are due to the differences in the collagen fibril arrangement in the deep zone.
34474320	6	95	theme	proteoglycans	1215:1227	arg1	contribution					1199:1210	the contribution	1195:1210	the contribution of proteoglycans and collagen network	1195:1248	Firstly, in order to quantify the contribution of proteoglycans and collagen network, the indentation testings on normal, proteoglycan, and collagen-degraded kangaroo knee cartilage were conducted at different strain-rates.
34474320	13	96	theme	collagen	2612:2619	arg1	network					2621:2627	the collagen network	2608:2627	the collagen network in the deep zone	2608:2644	From these results, it can be concluded that in high-load-bearing cartilage tissues, the collagen network in the deep zone assists in increasing the stiffness of tissue with strain-rate and plays a significant role in supporting transient loads.
34474320	14	97	from	importance	2898:2907	arg1	zone					2941:2944	deep zone	2936:2944	deep zone in assisting high-load-bearing cartilage tissues	2936:2993	This, in turn, helps protect the solid matrix against large distortions and strains at the subchondral junction, pointing to the importance of the collagen network in deep zone in assisting high-load-bearing cartilage tissues.
34474320	8	98	from	differences	1600:1610	arg1	architecture					1628:1639	collagen architecture	1619:1639	collagen architecture	1619:1639	Identified differences in the collagen architecture and proteoglycan composition were incorporated in a fibril-reinforced porohyperelastic Finite Element (FE) model with the objective of explaining the mechanisms underlying differences observed between the two tissues.
34474320	8	98	from	differences	1600:1610	arg1	composition					1658:1668	proteoglycan composition	1645:1668	proteoglycan composition	1645:1668	Identified differences in the collagen architecture and proteoglycan composition were incorporated in a fibril-reinforced porohyperelastic Finite Element (FE) model with the objective of explaining the mechanisms underlying differences observed between the two tissues.
34474320	5	99	theme	cartilage	1055:1063	arg1	behaviour					1024:1032	the strain-rate-dependent behaviour	998:1032	the strain-rate-dependent behaviour of the kangaroo knee cartilage	998:1063	Therefore, in the present study, we experimentally investigate the contribution of proteoglycan and collagen network to the strain-rate-dependent behaviour of the kangaroo knee cartilage, and plausible reasons for the differences observed in relation to the kangaroo shoulder cartilage.
34474320	6	100	from	testings	1267:1274	arg1	cartilage					1337:1345	normal, proteoglycan, and collagen-degraded kangaroo knee cartilage	1279:1345	normal, proteoglycan, and collagen-degraded kangaroo knee cartilage	1279:1345	Firstly, in order to quantify the contribution of proteoglycans and collagen network, the indentation testings on normal, proteoglycan, and collagen-degraded kangaroo knee cartilage were conducted at different strain-rates.
34474320	2	101	theme	strain-rate	397:407	arg1	increases					409:417	the strain-rate increases	393:417	the strain-rate increases	393:417	On the other hand, the contribution from the collagen network has been suggested to increase as the strain-rate increases.
34474320	5	102	theme	strain-rate-dependent	1002:1022	arg1	behaviour					1024:1032	the strain-rate-dependent behaviour	998:1032	the strain-rate-dependent behaviour of the kangaroo knee cartilage	998:1063	Therefore, in the present study, we experimentally investigate the contribution of proteoglycan and collagen network to the strain-rate-dependent behaviour of the kangaroo knee cartilage, and plausible reasons for the differences observed in relation to the kangaroo shoulder cartilage.
34474320	7	103	theme	compositional	1410:1422	arg1	differences					1424:1434	structural and compositional differences	1395:1434	structural and compositional differences between the kangaroo knee and shoulder cartilage	1395:1483	Then, structural and compositional differences between the kangaroo knee and shoulder cartilage were assessed qualitatively through polarised light microscopy (PLM) imaging and histological staining.
34474320	8	104	theme	fibril-reinforced	1693:1709	arg1	model					1748:1752	a fibril-reinforced porohyperelastic Finite Element (FE) model	1691:1752	a fibril-reinforced porohyperelastic Finite Element (FE) model with the objective of explaining the mechanisms underlying differences observed between the two tissues	1691:1856	Identified differences in the collagen architecture and proteoglycan composition were incorporated in a fibril-reinforced porohyperelastic Finite Element (FE) model with the objective of explaining the mechanisms underlying differences observed between the two tissues.
34474320	7	105	theme	structural	1395:1404	arg1	differences					1424:1434	structural and compositional differences	1395:1434	structural and compositional differences between the kangaroo knee and shoulder cartilage	1395:1483	Then, structural and compositional differences between the kangaroo knee and shoulder cartilage were assessed qualitatively through polarised light microscopy (PLM) imaging and histological staining.
34474320	8	106	theme	Finite	1728:1733	arg1	model					1748:1752	a fibril-reinforced porohyperelastic Finite Element (FE) model	1691:1752	a fibril-reinforced porohyperelastic Finite Element (FE) model with the objective of explaining the mechanisms underlying differences observed between the two tissues	1691:1856	Identified differences in the collagen architecture and proteoglycan composition were incorporated in a fibril-reinforced porohyperelastic Finite Element (FE) model with the objective of explaining the mechanisms underlying differences observed between the two tissues.
34474320	0	107	theme	mechanical	68:77	arg1	behaviour					79:87	the strain-rate-dependent mechanical behaviour	42:87	the strain-rate-dependent mechanical behaviour of knee and shoulder cartilage	42:118	Proteoglycan and collagen contribution to the strain-rate-dependent mechanical behaviour of knee and shoulder cartilage.
34474320	14	108	theme	subchondral	2860:2870	arg1	junction					2872:2879	the subchondral junction	2856:2879	the subchondral junction	2856:2879	This, in turn, helps protect the solid matrix against large distortions and strains at the subchondral junction, pointing to the importance of the collagen network in deep zone in assisting high-load-bearing cartilage tissues.
34474320	12	109	theme	biomechanical	2373:2385	arg1	due					2447:2449	due	2447:2449	due	2447:2449	FE model confirmed that the biomechanical differences observed in the knee and shoulder cartilage are due to the differences in the collagen fibril arrangement in the deep zone.
34474320	12	109	theme	biomechanical	2373:2385	arg1	differences					2387:2397	the biomechanical differences	2369:2397	the biomechanical differences observed in the knee and shoulder cartilage	2369:2441	FE model confirmed that the biomechanical differences observed in the knee and shoulder cartilage are due to the differences in the collagen fibril arrangement in the deep zone.
34474320	3	110	theme	knee	513:516	arg1	tissues					528:534	high-load-bearing knee cartilage tissues	495:534	high-load-bearing knee cartilage tissues	495:534	These conclusions are drawn mainly based on numerical studies conducted on high-load-bearing knee cartilage tissues, while experimental evidence of these behaviours have not been demonstrated previously.
34474320	4	111	theme	reported	652:659	arg1	findings					661:668	the reported findings	648:668	the reported findings on high-load bearing knee cartilage	648:704	Further, in contrast to the reported findings on high-load bearing knee cartilage, our previous study on the low-load-bearing kangaroo shoulder cartilage indicated that proteoglycan and collagen contribution remained steady as the strain-rate increases.
34474320	4	112	theme	strain-rate	855:865	arg1	increases					867:875	the strain-rate increases	851:875	the strain-rate increases	851:875	Further, in contrast to the reported findings on high-load bearing knee cartilage, our previous study on the low-load-bearing kangaroo shoulder cartilage indicated that proteoglycan and collagen contribution remained steady as the strain-rate increases.
34474320	5	113	located	observed	1108:1115	arg1	relation					1120:1127	relation	1120:1127	relation to the kangaroo shoulder cartilage	1120:1162	Therefore, in the present study, we experimentally investigate the contribution of proteoglycan and collagen network to the strain-rate-dependent behaviour of the kangaroo knee cartilage, and plausible reasons for the differences observed in relation to the kangaroo shoulder cartilage.
34474320	5	113	located	observed	1108:1115	arg2	differences					1096:1106	the differences	1092:1106	the differences observed in relation to the kangaroo shoulder cartilage	1092:1162	Therefore, in the present study, we experimentally investigate the contribution of proteoglycan and collagen network to the strain-rate-dependent behaviour of the kangaroo knee cartilage, and plausible reasons for the differences observed in relation to the kangaroo shoulder cartilage.
34082873	1	0	theme	fibrosus	396:403	arg1	tissue					410:415	annulus fibrosus (AF) tissue	388:415	annulus fibrosus (AF) tissue	388:415	Tissue engineering is a promising approach for the treatment of chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue.
34082873	7	1	theme	PLGA	1146:1149	arg1	g					1154:1154	PLGA 10 g	1146:1154	PLGA 10 g	1146:1154	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	7	1	theme	PLGA	1146:1149	arg1	g					1172:1172	DEX 2 g	1166:1172	DEX 2 g	1166:1172	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	7	1	theme	PLGA	1146:1149	arg1	g					1163:1163	PCL 8 g	1157:1163	PCL 8 g	1157:1163	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	7	2	theme	uniform	1223:1229	arg1	distribution					1240:1251	the most uniform diameter distribution	1214:1251	the most uniform diameter distribution	1214:1251	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	1	3	theme	intervertebral	301:314	arg1	IDD					335:337	IDD	335:337	IDD	335:337	Tissue engineering is a promising approach for the treatment of chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue.
34082873	1	3	theme	intervertebral	301:314	arg1	degeneration					321:332	intervertebral disc degeneration	301:332	intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue	301:415	Tissue engineering is a promising approach for the treatment of chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue.
34082873	3	4	theme	herbal	701:706	arg1	extract					708:714	a Traditional Chinese Medicine herbal extract	670:714	a Traditional Chinese Medicine herbal extract	670:714	In this study, we fabricated a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning.
34082873	3	4	theme	herbal	701:706	arg1	extract					655:661	plastrum testudinis extract	635:661	plastrum testudinis extract (PTE)	635:667	In this study, we fabricated a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning.
34082873	5	5	dep	in	847:848	arg1	vitro					850:854	vitro	850:854	vitro	850:854	Using an in vitro inflammation model induced by interleukin (IL)-1β, the cytocompatibility and anti-inflammatory effects of the composites were investigated by CCK-8 assay and flow cytometry.
34082873	7	6	theme	diameter	1231:1238	arg1	distribution					1240:1251	the most uniform diameter distribution	1214:1251	the most uniform diameter distribution	1214:1251	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	1	7	theme	disc	316:319	arg1	IDD					335:337	IDD	335:337	IDD	335:337	Tissue engineering is a promising approach for the treatment of chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue.
34082873	1	7	theme	disc	316:319	arg1	degeneration					321:332	intervertebral disc degeneration	301:332	intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue	301:415	Tissue engineering is a promising approach for the treatment of chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue.
34082873	0	8	theme	Annulus	166:172	arg1	Regeneration					183:194	Annulus Fibrosus Regeneration	166:194	Annulus Fibrosus Regeneration	166:194	Fabrication of a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane via Electrospinning for Annulus Fibrosus Regeneration.
34082873	7	9	theme	P10P8D2	1137:1143	arg1	membrane					1195:1202	the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane	1133:1202	the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane	1133:1202	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	7	10	theme	nanofiber	1185:1193	arg1	membrane					1195:1202	the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane	1133:1202	the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane	1133:1202	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	12	11	theme	effective	1745:1753	arg1	strategy					1755:1762	an effective strategy	1742:1762	an effective strategy for AF tissue regeneration	1742:1789	It may provide an effective strategy for AF tissue regeneration.
34082873	1	12	theme	tissue	410:415	arg1	inflammation					372:383	inflammation	372:383	inflammation	372:383	Tissue engineering is a promising approach for the treatment of chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue.
34082873	1	12	theme	tissue	410:415	arg1	degeneration					355:366	degeneration	355:366	degeneration	355:366	Tissue engineering is a promising approach for the treatment of chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue.
34082873	9	13	theme	cell	1478:1481	arg1	proliferation					1483:1495	cell proliferation	1478:1495	cell proliferation	1478:1495	Importantly, P10P8D2 combined with PTE exhibited anti-inflammatory and cell proliferation promotion effects.
34082873	4	14	theme	electron	811:818	arg1	SEM					832:834	SEM	832:834	SEM	832:834	The membranes were characterized by mechanical measurements and scanning electron microscopy (SEM).
34082873	4	14	theme	electron	811:818	arg1	microscopy					820:829	scanning electron microscopy	802:829	scanning electron microscopy (SEM)	802:835	The membranes were characterized by mechanical measurements and scanning electron microscopy (SEM).
34082873	8	15	theme	PTE	1388:1390	arg1	µg/mL					1400:1404	120 µg/mL	1396:1404	120 µg/mL	1396:1404	The optimal concentration of PTE was 120 µg/mL.
34082873	8	15	theme	PTE	1388:1390	arg1	concentration					1371:1383	The optimal concentration	1359:1383	The optimal concentration of PTE	1359:1390	The optimal concentration of PTE was 120 µg/mL.
34082873	11	16	theme	pro-proliferation	1688:1704	arg1	effects					1706:1712	anti-inflammatory and pro-proliferation effects	1666:1712	anti-inflammatory and pro-proliferation effects	1666:1712	Our findings suggested that the nanofiber membrane composed of P10P8D2 and PTE has anti-inflammatory and pro-proliferation effects on AF cells.
34082873	0	17	theme	Fibrosus	174:181	arg1	Regeneration					183:194	Annulus Fibrosus Regeneration	166:194	Annulus Fibrosus Regeneration	166:194	Fabrication of a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane via Electrospinning for Annulus Fibrosus Regeneration.
34082873	7	18	theme	PCL	1157:1159	arg1	g					1154:1154	PLGA 10 g	1146:1154	PLGA 10 g	1146:1154	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	7	18	theme	PCL	1157:1159	arg1	g					1163:1163	PCL 8 g	1157:1163	PCL 8 g	1157:1163	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	3	19	theme	/poly-ε-caprolactone	563:582	arg1	Zdextran					589:596	a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran	533:596	a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning	533:735	In this study, we fabricated a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning.
34082873	3	19	theme	/poly-ε-caprolactone	563:582	arg1	DEX					599:601	DEX	599:601	DEX	599:601	In this study, we fabricated a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning.
34082873	5	20	theme	interleukin	886:896	arg1	IL					899:900	interleukin (IL)-1β	886:904	interleukin (IL)-1β	886:904	Using an in vitro inflammation model induced by interleukin (IL)-1β, the cytocompatibility and anti-inflammatory effects of the composites were investigated by CCK-8 assay and flow cytometry.
34082873	7	21	theme	DEX	1166:1168	arg1	g					1172:1172	DEX 2 g	1166:1172	DEX 2 g	1166:1172	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	7	21	theme	DEX	1166:1168	arg1	g					1154:1154	PLGA 10 g	1146:1154	PLGA 10 g	1146:1154	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	11	22	theme	AF	1717:1718	arg1	cells					1720:1724	AF cells	1717:1724	AF cells	1717:1724	Our findings suggested that the nanofiber membrane composed of P10P8D2 and PTE has anti-inflammatory and pro-proliferation effects on AF cells.
34082873	12	23	theme	tissue	1771:1776	arg1	regeneration					1778:1789	AF tissue regeneration	1768:1789	AF tissue regeneration	1768:1789	It may provide an effective strategy for AF tissue regeneration.
34082873	10	24	theme	signaling	1548:1556	arg1	pathway					1558:1564	the NF-κBB/NLRP3/IL-β signaling pathway	1526:1564	the NF-κBB/NLRP3/IL-β signaling pathway	1526:1564	Moreover, the NF-κBB/NLRP3/IL-β signaling pathway was inactivated.
34082873	11	25	theme	anti-inflammatory	1666:1682	arg1	effects					1706:1712	anti-inflammatory and pro-proliferation effects	1666:1712	anti-inflammatory and pro-proliferation effects	1666:1712	Our findings suggested that the nanofiber membrane composed of P10P8D2 and PTE has anti-inflammatory and pro-proliferation effects on AF cells.
34082873	8	26	theme	optimal	1363:1369	arg1	µg/mL					1400:1404	120 µg/mL	1396:1404	120 µg/mL	1396:1404	The optimal concentration of PTE was 120 µg/mL.
34082873	8	26	theme	optimal	1363:1369	arg1	concentration					1371:1383	The optimal concentration	1359:1383	The optimal concentration of PTE	1359:1390	The optimal concentration of PTE was 120 µg/mL.
34082873	7	27	theme	moderate	1284:1291	arg1	rate					1305:1308	a moderate degradation rate	1282:1308	a moderate degradation rate	1282:1308	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	1	28	theme	chronic	261:267	arg1	LBP					286:288	LBP	286:288	LBP	286:288	Tissue engineering is a promising approach for the treatment of chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue.
34082873	1	28	theme	chronic	261:267	arg1	pain					280:283	back pain	275:283	chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue	261:415	Tissue engineering is a promising approach for the treatment of chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue.
34082873	0	29	theme	Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum	17:74	arg1	Membrane					133:140	a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane	15:140	a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane	15:140	Fabrication of a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane via Electrospinning for Annulus Fibrosus Regeneration.
34082873	7	30	dep	membrane	1195:1202	arg1	g					1154:1154	PLGA 10 g	1146:1154	PLGA 10 g	1146:1154	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	7	30	dep	membrane	1195:1202	arg1	g					1172:1172	DEX 2 g	1166:1172	DEX 2 g	1166:1172	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	7	30	dep	membrane	1195:1202	arg1	g					1163:1163	PCL 8 g	1157:1163	PCL 8 g	1157:1163	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	4	31	theme	mechanical	774:783	arg1	measurements					785:796	mechanical measurements	774:796	mechanical measurements	774:796	The membranes were characterized by mechanical measurements and scanning electron microscopy (SEM).
34082873	2	32	theme	AF	472:473	arg1	cells					475:479	AF cells	472:479	AF cells	472:479	However, scaffold with an anti-inflammatory effect on AF cells has not been reported.
34082873	5	33	theme	in	847:848	arg1	model					869:873	an in vitro inflammation model	844:873	an in vitro inflammation model induced by interleukin (IL)-1β	844:904	Using an in vitro inflammation model induced by interleukin (IL)-1β, the cytocompatibility and anti-inflammatory effects of the composites were investigated by CCK-8 assay and flow cytometry.
34082873	11	34	contain	has	1662:1664	arg2	effects					1706:1712	anti-inflammatory and pro-proliferation effects	1666:1712	anti-inflammatory and pro-proliferation effects	1666:1712	Our findings suggested that the nanofiber membrane composed of P10P8D2 and PTE has anti-inflammatory and pro-proliferation effects on AF cells.
34082873	11	34	contain	has	1662:1664	arg1	membrane					1625:1632	the nanofiber membrane	1611:1632	the nanofiber membrane composed of P10P8D2 and PTE	1611:1660	Our findings suggested that the nanofiber membrane composed of P10P8D2 and PTE has anti-inflammatory and pro-proliferation effects on AF cells.
34082873	5	35	theme	CCK-8	998:1002	arg1	assay					1004:1008	CCK-8 assay	998:1008	CCK-8 assay	998:1008	Using an in vitro inflammation model induced by interleukin (IL)-1β, the cytocompatibility and anti-inflammatory effects of the composites were investigated by CCK-8 assay and flow cytometry.
34082873	3	36	theme	Zdextran	589:596	arg1	membrane					614:621	a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane	533:621	a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning	533:735	In this study, we fabricated a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning.
34082873	5	37	theme	inflammation	856:867	arg1	model					869:873	an in vitro inflammation model	844:873	an in vitro inflammation model induced by interleukin (IL)-1β	844:904	Using an in vitro inflammation model induced by interleukin (IL)-1β, the cytocompatibility and anti-inflammatory effects of the composites were investigated by CCK-8 assay and flow cytometry.
34082873	0	38	theme	Extract	87:93	arg1	Membrane					133:140	a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane	15:140	a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane	15:140	Fabrication of a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane via Electrospinning for Annulus Fibrosus Regeneration.
34082873	3	39	dep	/poly-ε-caprolactone	563:582	arg1	PCL					585:587	PCL	585:587	PCL	585:587	In this study, we fabricated a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning.
34082873	6	40	theme	Western	1091:1097	arg1	blotting					1099:1106	Western blotting	1091:1106	Western blotting	1091:1106	Potential regulatory mechanisms were examined by RT-qPCR and Western blotting.
34082873	1	41	theme	pain	280:283	arg1	treatment					248:256	the treatment	244:256	the treatment of chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue	244:415	Tissue engineering is a promising approach for the treatment of chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue.
34082873	0	42	theme	Testudinis	76:85	arg1	Membrane					133:140	a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane	15:140	a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane	15:140	Fabrication of a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane via Electrospinning for Annulus Fibrosus Regeneration.
34082873	9	43	theme	promotion	1497:1505	arg1	effects					1507:1513	promotion effects	1497:1513	promotion effects	1497:1513	Importantly, P10P8D2 combined with PTE exhibited anti-inflammatory and cell proliferation promotion effects.
34082873	1	44	dep	chronic	261:267	arg1	lower					269:273	lower	269:273	lower	269:273	Tissue engineering is a promising approach for the treatment of chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue.
34082873	2	45	theme	anti-inflammatory	444:460	arg1	effect					462:467	an anti-inflammatory effect	441:467	an anti-inflammatory effect on AF cells	441:479	However, scaffold with an anti-inflammatory effect on AF cells has not been reported.
34082873	0	46	theme	Anti-Inflammation	105:121	arg1	Membrane					133:140	a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane	15:140	a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane	15:140	Fabrication of a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane via Electrospinning for Annulus Fibrosus Regeneration.
34082873	7	47	theme	composite	1175:1183	arg1	membrane					1195:1202	the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane	1133:1202	the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane	1133:1202	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	5	48	theme	cytocompatibility	911:927	arg1	effects					951:957	the cytocompatibility and anti-inflammatory effects	907:957	the cytocompatibility and anti-inflammatory effects of the composites	907:975	Using an in vitro inflammation model induced by interleukin (IL)-1β, the cytocompatibility and anti-inflammatory effects of the composites were investigated by CCK-8 assay and flow cytometry.
34082873	0	49	theme	Composite	95:103	arg1	Membrane					133:140	a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane	15:140	a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane	15:140	Fabrication of a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane via Electrospinning for Annulus Fibrosus Regeneration.
34082873	2	50	with	scaffold	427:434	arg1	effect					462:467	an anti-inflammatory effect	441:467	an anti-inflammatory effect on AF cells	441:479	However, scaffold with an anti-inflammatory effect on AF cells has not been reported.
34082873	3	51	theme	Chinese	684:690	arg1	extract					708:714	a Traditional Chinese Medicine herbal extract	670:714	a Traditional Chinese Medicine herbal extract	670:714	In this study, we fabricated a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning.
34082873	3	51	theme	Chinese	684:690	arg1	extract					655:661	plastrum testudinis extract	635:661	plastrum testudinis extract (PTE)	635:667	In this study, we fabricated a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning.
34082873	3	52	theme	composite	604:612	arg1	membrane					614:621	a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane	533:621	a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning	533:735	In this study, we fabricated a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning.
34082873	11	53	theme	nanofiber	1615:1623	arg1	membrane					1625:1632	the nanofiber membrane	1611:1632	the nanofiber membrane composed of P10P8D2 and PTE	1611:1660	Our findings suggested that the nanofiber membrane composed of P10P8D2 and PTE has anti-inflammatory and pro-proliferation effects on AF cells.
34082873	1	54	theme	Tissue	197:202	arg1	engineering					204:214	Tissue engineering	197:214	Tissue engineering	197:214	Tissue engineering is a promising approach for the treatment of chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue.
34082873	1	54	theme	Tissue	197:202	arg1	approach					231:238	a promising approach	219:238	a promising approach for the treatment of chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue	219:415	Tissue engineering is a promising approach for the treatment of chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue.
34082873	10	55	theme	NF-κBB/NLRP3/IL-β	1530:1546	arg1	pathway					1558:1564	the NF-κBB/NLRP3/IL-β signaling pathway	1526:1564	the NF-κBB/NLRP3/IL-β signaling pathway	1526:1564	Moreover, the NF-κBB/NLRP3/IL-β signaling pathway was inactivated.
34082873	3	56	theme	Medicine	692:699	arg1	extract					708:714	a Traditional Chinese Medicine herbal extract	670:714	a Traditional Chinese Medicine herbal extract	670:714	In this study, we fabricated a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning.
34082873	3	56	theme	Medicine	692:699	arg1	extract					655:661	plastrum testudinis extract	635:661	plastrum testudinis extract (PTE)	635:667	In this study, we fabricated a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning.
34082873	3	57	theme	testudinis	644:653	arg1	PTE					664:666	PTE	664:666	PTE	664:666	In this study, we fabricated a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning.
34082873	3	57	theme	testudinis	644:653	arg1	extract					708:714	a Traditional Chinese Medicine herbal extract	670:714	a Traditional Chinese Medicine herbal extract	670:714	In this study, we fabricated a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning.
34082873	3	57	theme	testudinis	644:653	arg1	extract					655:661	plastrum testudinis extract	635:661	plastrum testudinis extract (PTE)	635:667	In this study, we fabricated a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning.
34082873	9	58	dep	anti-inflammatory	1456:1472	arg1	effects					1507:1513	promotion effects	1497:1513	promotion effects	1497:1513	Importantly, P10P8D2 combined with PTE exhibited anti-inflammatory and cell proliferation promotion effects.
34082873	5	59	theme	anti-inflammatory	933:949	arg1	effects					951:957	the cytocompatibility and anti-inflammatory effects	907:957	the cytocompatibility and anti-inflammatory effects of the composites	907:975	Using an in vitro inflammation model induced by interleukin (IL)-1β, the cytocompatibility and anti-inflammatory effects of the composites were investigated by CCK-8 assay and flow cytometry.
34082873	5	60	theme	composites	966:975	arg1	effects					951:957	the cytocompatibility and anti-inflammatory effects	907:957	the cytocompatibility and anti-inflammatory effects of the composites	907:975	Using an in vitro inflammation model induced by interleukin (IL)-1β, the cytocompatibility and anti-inflammatory effects of the composites were investigated by CCK-8 assay and flow cytometry.
34082873	4	61	theme	scanning	802:809	arg1	SEM					832:834	SEM	832:834	SEM	832:834	The membranes were characterized by mechanical measurements and scanning electron microscopy (SEM).
34082873	4	61	theme	scanning	802:809	arg1	microscopy					820:829	scanning electron microscopy	802:829	scanning electron microscopy (SEM)	802:835	The membranes were characterized by mechanical measurements and scanning electron microscopy (SEM).
34082873	5	62	theme	flow	1014:1017	arg1	cytometry					1019:1027	flow cytometry	1014:1027	flow cytometry	1014:1027	Using an in vitro inflammation model induced by interleukin (IL)-1β, the cytocompatibility and anti-inflammatory effects of the composites were investigated by CCK-8 assay and flow cytometry.
34082873	2	63	from	effect	462:467	arg1	cells					475:479	AF cells	472:479	AF cells	472:479	However, scaffold with an anti-inflammatory effect on AF cells has not been reported.
34082873	7	64	theme	mechanical	1259:1268	arg1	properties					1270:1279	best mechanical properties	1254:1279	best mechanical properties	1254:1279	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	12	65	theme	AF	1768:1769	arg1	regeneration					1778:1789	AF tissue regeneration	1768:1789	AF tissue regeneration	1768:1789	It may provide an effective strategy for AF tissue regeneration.
34082873	7	66	theme	best	1254:1257	arg1	properties					1270:1279	best mechanical properties	1254:1279	best mechanical properties	1254:1279	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	0	67	theme	Membrane	133:140	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane via Electrospinning for Annulus Fibrosus Regeneration.	0:195	Fabrication of a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane via Electrospinning for Annulus Fibrosus Regeneration.
34082873	3	68	theme	Traditional	672:682	arg1	extract					708:714	a Traditional Chinese Medicine herbal extract	670:714	a Traditional Chinese Medicine herbal extract	670:714	In this study, we fabricated a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning.
34082873	3	68	theme	Traditional	672:682	arg1	extract					655:661	plastrum testudinis extract	635:661	plastrum testudinis extract (PTE)	635:667	In this study, we fabricated a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning.
34082873	7	69	theme	cytocompatibility	1324:1340	arg1	characteristics					1342:1356	the best cytocompatibility characteristics	1315:1356	the best cytocompatibility characteristics	1315:1356	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	7	70	theme	degradation	1293:1303	arg1	rate					1305:1308	a moderate degradation rate	1282:1308	a moderate degradation rate	1282:1308	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	7	71	theme	best	1319:1322	arg1	characteristics					1342:1356	the best cytocompatibility characteristics	1315:1356	the best cytocompatibility characteristics	1315:1356	The results showed that the P10P8D2 (PLGA 10 g, PCL 8 g, DEX 2 g) composite nanofiber membrane exhibited the most uniform diameter distribution, best mechanical properties, a moderate degradation rate, and the best cytocompatibility characteristics.
34082873	6	72	theme	regulatory	1040:1049	arg1	mechanisms					1051:1060	Potential regulatory mechanisms	1030:1060	Potential regulatory mechanisms	1030:1060	Potential regulatory mechanisms were examined by RT-qPCR and Western blotting.
34082873	0	73	theme	Nanofiber	123:131	arg1	Membrane					133:140	a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane	15:140	a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane	15:140	Fabrication of a Polylactide-Glycolide/Poly-ε-Caprolactone/Dextran/Plastrum Testudinis Extract Composite Anti-Inflammation Nanofiber Membrane via Electrospinning for Annulus Fibrosus Regeneration.
34082873	1	74	theme	annulus	388:394	arg1	AF					406:407	AF	406:407	AF	406:407	Tissue engineering is a promising approach for the treatment of chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue.
34082873	1	74	theme	annulus	388:394	arg1	fibrosus					396:403	annulus fibrosus	388:403	annulus fibrosus (AF) tissue	388:415	Tissue engineering is a promising approach for the treatment of chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue.
34082873	3	75	theme	plastrum	635:642	arg1	PTE					664:666	PTE	664:666	PTE	664:666	In this study, we fabricated a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning.
34082873	3	75	theme	plastrum	635:642	arg1	extract					708:714	a Traditional Chinese Medicine herbal extract	670:714	a Traditional Chinese Medicine herbal extract	670:714	In this study, we fabricated a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning.
34082873	3	75	theme	plastrum	635:642	arg1	extract					655:661	plastrum testudinis extract	635:661	plastrum testudinis extract (PTE)	635:667	In this study, we fabricated a polylactide-glycolide (PLGA)/poly-ε-caprolactone (PCL)Zdextran (DEX) composite membrane loaded with plastrum testudinis extract (PTE), a Traditional Chinese Medicine herbal extract, via electrospinning.
34082873	1	76	theme	promising	221:229	arg1	engineering					204:214	Tissue engineering	197:214	Tissue engineering	197:214	Tissue engineering is a promising approach for the treatment of chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue.
34082873	1	76	theme	promising	221:229	arg1	approach					231:238	a promising approach	219:238	a promising approach for the treatment of chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue	219:415	Tissue engineering is a promising approach for the treatment of chronic lower back pain (LBP) caused by intervertebral disc degeneration (IDD) resulting from degeneration and inflammation of annulus fibrosus (AF) tissue.
34082873	6	77	theme	Potential	1030:1038	arg1	mechanisms					1051:1060	Potential regulatory mechanisms	1030:1060	Potential regulatory mechanisms	1030:1060	Potential regulatory mechanisms were examined by RT-qPCR and Western blotting.
33607408	6	0	theme	SGF	1100:1102	arg1	digestion					1104:1112	SGF digestion	1100:1112	SGF digestion	1100:1112	Additionally, EPS-SeNPs (Se/EPS = 1/20) showed a low Se release after SGF digestion, but a high release after SIF digestion, suggesting its application in controlled release of Se-enriched supplements for Se-deficiency treatment.
33607408	3	1	contain	had	610:612	arg1	ratio					597:601	ratio	597:601	ratio	597:601	Se/EPS ratio and pH had significant influence on Se release.
33607408	3	1	contain	had	610:612	arg1	Se/EPS					590:595	Se/EPS ratio and pH	590:608	Se/EPS ratio and pH	590:608	Se/EPS ratio and pH had significant influence on Se release.
33607408	3	1	contain	had	610:612	arg1	pH					607:608	pH	607:608	pH	607:608	Se/EPS ratio and pH had significant influence on Se release.
33607408	3	1	contain	had	610:612	arg2	influence					626:634	significant influence	614:634	significant influence	614:634	Se/EPS ratio and pH had significant influence on Se release.
33607408	2	2	dep	250-320 nm	517:526	arg1	80-125 nm					504:512	80-125 nm	504:512	80-125 nm	504:512	EPS-SeNPs showed an increase in size from 80-125 nm to 250-320 nm and more ambiguous boundary after gastrointestinal digestion.
33607408	1	3	theme	release	194:200	arg1	kinetics					202:209	selenium (Se) release kinetics	180:209	selenium (Se) release kinetics	180:209	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	6	4	from	application	1170:1180	arg1	release					1196:1202	controlled release	1185:1202	controlled release of Se-enriched supplements	1185:1229	Additionally, EPS-SeNPs (Se/EPS = 1/20) showed a low Se release after SGF digestion, but a high release after SIF digestion, suggesting its application in controlled release of Se-enriched supplements for Se-deficiency treatment.
33607408	0	5	theme	gastrointestinal	128:143	arg1	conditions					145:154	simulated gastrointestinal conditions	118:154	simulated gastrointestinal conditions	118:154	Selenium release kinetics and mechanism from Cordyceps sinensis exopolysaccharide-selenium composite nanoparticles in simulated gastrointestinal conditions.
33607408	1	6	dep	gastric	324:330	arg1	SGF					333:335	SGF	333:335	SGF	333:335	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	1	7	theme	Korsmeyer-Peppas	444:459	arg1	models					381:386	kinetics models	372:386	kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas	372:459	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	5	8	theme	non-Fickian	966:976	arg1	mechanism					978:986	a non-Fickian mechanism	964:986	a non-Fickian mechanism controlled by both diffusion and erosion	964:1027	Se release from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SIF) was well-fitted to Korsmeyer-Peppas model and followed a non-Fickian mechanism controlled by both diffusion and erosion.
33607408	4	9	from	4/3	704:706	arg1	SGF					711:713	SGF	711:713	SGF	711:713	Se release kinetics from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SGF) followed a classical Fickian diffusion, in contrast to an erosion governed by macromolecular chains relaxation for Se/EPS = 1/20 in SIF.
33607408	0	10	theme	simulated	118:126	arg1	conditions					145:154	simulated gastrointestinal conditions	118:154	simulated gastrointestinal conditions	118:154	Selenium release kinetics and mechanism from Cordyceps sinensis exopolysaccharide-selenium composite nanoparticles in simulated gastrointestinal conditions.
33607408	1	11	theme	kinetics	372:379	arg1	models					381:386	kinetics models	372:386	kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas	372:459	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	5	12	from	4/3	897:899	arg1	SIF					904:906	SIF	904:906	SIF	904:906	Se release from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SIF) was well-fitted to Korsmeyer-Peppas model and followed a non-Fickian mechanism controlled by both diffusion and erosion.
33607408	4	13	theme	classical	727:735	arg1	diffusion					745:753	a classical Fickian diffusion	725:753	a classical Fickian diffusion	725:753	Se release kinetics from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SGF) followed a classical Fickian diffusion, in contrast to an erosion governed by macromolecular chains relaxation for Se/EPS = 1/20 in SIF.
33607408	4	14	dep	EPS-SeNPs	676:684	arg1	4/3					704:706	4/3	704:706	4/3	704:706	Se release kinetics from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SGF) followed a classical Fickian diffusion, in contrast to an erosion governed by macromolecular chains relaxation for Se/EPS = 1/20 in SIF.
33607408	4	14	dep	EPS-SeNPs	676:684	arg1	Se/EPS = 1/1					687:698	Se/EPS = 1/1	687:698	Se/EPS = 1/1	687:698	Se release kinetics from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SGF) followed a classical Fickian diffusion, in contrast to an erosion governed by macromolecular chains relaxation for Se/EPS = 1/20 in SIF.
33607408	5	15	theme	Se	853:854	arg1	release					856:862	Se release	853:862	Se release from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SIF)	853:907	Se release from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SIF) was well-fitted to Korsmeyer-Peppas model and followed a non-Fickian mechanism controlled by both diffusion and erosion.
33607408	4	16	from	Se/EPS = 1/1	687:698	arg1	SGF					711:713	SGF	711:713	SGF	711:713	Se release kinetics from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SGF) followed a classical Fickian diffusion, in contrast to an erosion governed by macromolecular chains relaxation for Se/EPS = 1/20 in SIF.
33607408	6	17	theme	Se	1083:1084	arg1	release					1086:1092	a low Se release	1077:1092	a low Se release	1077:1092	Additionally, EPS-SeNPs (Se/EPS = 1/20) showed a low Se release after SGF digestion, but a high release after SIF digestion, suggesting its application in controlled release of Se-enriched supplements for Se-deficiency treatment.
33607408	2	18	from	increase	482:489	arg1	size					494:497	size	494:497	size	494:497	EPS-SeNPs showed an increase in size from 80-125 nm to 250-320 nm and more ambiguous boundary after gastrointestinal digestion.
33607408	4	19	from	EPS-SeNPs	676:684	arg1	kinetics					662:669	Se release kinetics	651:669	Se release kinetics from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SGF)	651:714	Se release kinetics from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SGF) followed a classical Fickian diffusion, in contrast to an erosion governed by macromolecular chains relaxation for Se/EPS = 1/20 in SIF.
33607408	6	20	theme	low	1079:1081	arg1	release					1086:1092	a low Se release	1077:1092	a low Se release	1077:1092	Additionally, EPS-SeNPs (Se/EPS = 1/20) showed a low Se release after SGF digestion, but a high release after SIF digestion, suggesting its application in controlled release of Se-enriched supplements for Se-deficiency treatment.
33607408	1	21	theme	simulated	314:322	arg1	SIF					361:363	SIF	361:363	SIF	361:363	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	1	21	theme	simulated	314:322	arg1	fluids					353:358	simulated gastric (SGF) and intestinal fluids	314:358	simulated gastric (SGF) and intestinal fluids (SIF)	314:364	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	1	22	theme	gastric	324:330	arg1	SIF					361:363	SIF	361:363	SIF	361:363	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	1	22	theme	gastric	324:330	arg1	fluids					353:358	simulated gastric (SGF) and intestinal fluids	314:358	simulated gastric (SGF) and intestinal fluids (SIF)	314:364	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	5	23	theme	Korsmeyer-Peppas	928:943	arg1	model					945:949	Korsmeyer-Peppas model	928:949	Korsmeyer-Peppas model	928:949	Se release from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SIF) was well-fitted to Korsmeyer-Peppas model and followed a non-Fickian mechanism controlled by both diffusion and erosion.
33607408	6	24	theme	SIF	1140:1142	arg1	digestion					1144:1152	SIF digestion	1140:1152	SIF digestion	1140:1152	Additionally, EPS-SeNPs (Se/EPS = 1/20) showed a low Se release after SGF digestion, but a high release after SIF digestion, suggesting its application in controlled release of Se-enriched supplements for Se-deficiency treatment.
33607408	1	25	theme	exopolysaccharide-selenium	230:255	arg1	nanoparticles					257:269	exopolysaccharide-selenium nanoparticles	230:269	exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF)	230:364	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	1	25	theme	exopolysaccharide-selenium	230:255	arg1	Se/EPS = 1/20					283:295	Se/EPS = 1/20	283:295	Se/EPS = 1/20	283:295	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	1	25	theme	exopolysaccharide-selenium	230:255	arg1	EPS-SeNPs					272:280	EPS-SeNPs	272:280	EPS-SeNPs	272:280	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	2	26	theme	gastrointestinal	562:577	arg1	digestion					579:587	gastrointestinal digestion	562:587	gastrointestinal digestion	562:587	EPS-SeNPs showed an increase in size from 80-125 nm to 250-320 nm and more ambiguous boundary after gastrointestinal digestion.
33607408	1	27	theme	order	396:400	arg1	models					381:386	kinetics models	372:386	kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas	372:459	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	4	28	theme	Se	651:652	arg1	kinetics					662:669	Se release kinetics	651:669	Se release kinetics from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SGF)	651:714	Se release kinetics from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SGF) followed a classical Fickian diffusion, in contrast to an erosion governed by macromolecular chains relaxation for Se/EPS = 1/20 in SIF.
33607408	0	29	from	nanoparticles	101:113	arg1	kinetics					17:24	kinetics	17:24	kinetics	17:24	Selenium release kinetics and mechanism from Cordyceps sinensis exopolysaccharide-selenium composite nanoparticles in simulated gastrointestinal conditions.
33607408	0	29	from	nanoparticles	101:113	arg1	mechanism					30:38	mechanism	30:38	mechanism	30:38	Selenium release kinetics and mechanism from Cordyceps sinensis exopolysaccharide-selenium composite nanoparticles in simulated gastrointestinal conditions.
33607408	3	30	theme	significant	614:624	arg1	influence					626:634	significant influence	614:634	significant influence	614:634	Se/EPS ratio and pH had significant influence on Se release.
33607408	6	31	theme	controlled	1185:1194	arg1	release					1196:1202	controlled release	1185:1202	controlled release of Se-enriched supplements	1185:1229	Additionally, EPS-SeNPs (Se/EPS = 1/20) showed a low Se release after SGF digestion, but a high release after SIF digestion, suggesting its application in controlled release of Se-enriched supplements for Se-deficiency treatment.
33607408	1	32	theme	First	403:407	arg1	order					409:413	First order	403:413	First order	403:413	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	6	33	theme	Se-deficiency	1235:1247	arg1	treatment					1249:1257	Se-deficiency treatment	1235:1257	Se-deficiency treatment	1235:1257	Additionally, EPS-SeNPs (Se/EPS = 1/20) showed a low Se release after SGF digestion, but a high release after SIF digestion, suggesting its application in controlled release of Se-enriched supplements for Se-deficiency treatment.
33607408	0	34	theme	sinensis	55:62	arg1	nanoparticles					101:113	Cordyceps sinensis exopolysaccharide-selenium composite nanoparticles	45:113	Cordyceps sinensis exopolysaccharide-selenium composite nanoparticles	45:113	Selenium release kinetics and mechanism from Cordyceps sinensis exopolysaccharide-selenium composite nanoparticles in simulated gastrointestinal conditions.
33607408	2	35	theme	ambiguous	537:545	arg1	boundary					547:554	more ambiguous boundary	532:554	more ambiguous boundary	532:554	EPS-SeNPs showed an increase in size from 80-125 nm to 250-320 nm and more ambiguous boundary after gastrointestinal digestion.
33607408	1	36	theme	order	409:413	arg1	models					381:386	kinetics models	372:386	kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas	372:459	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	5	37	dep	EPS-SeNPs	869:877	arg1	4/3					897:899	4/3	897:899	4/3	897:899	Se release from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SIF) was well-fitted to Korsmeyer-Peppas model and followed a non-Fickian mechanism controlled by both diffusion and erosion.
33607408	5	37	dep	EPS-SeNPs	869:877	arg1	Se/EPS = 1/1					880:891	Se/EPS = 1/1	880:891	Se/EPS = 1/1	880:891	Se release from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SIF) was well-fitted to Korsmeyer-Peppas model and followed a non-Fickian mechanism controlled by both diffusion and erosion.
33607408	0	38	theme	Cordyceps	45:53	arg1	nanoparticles					101:113	Cordyceps sinensis exopolysaccharide-selenium composite nanoparticles	45:113	Cordyceps sinensis exopolysaccharide-selenium composite nanoparticles	45:113	Selenium release kinetics and mechanism from Cordyceps sinensis exopolysaccharide-selenium composite nanoparticles in simulated gastrointestinal conditions.
33607408	1	39	theme	intestinal	342:351	arg1	SIF					361:363	SIF	361:363	SIF	361:363	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	1	39	theme	intestinal	342:351	arg1	fluids					353:358	simulated gastric (SGF) and intestinal fluids	314:358	simulated gastric (SGF) and intestinal fluids (SIF)	314:364	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	6	40	dep	release	1126:1132	arg1	digestion					1144:1152	SIF digestion	1140:1152	SIF digestion	1140:1152	Additionally, EPS-SeNPs (Se/EPS = 1/20) showed a low Se release after SGF digestion, but a high release after SIF digestion, suggesting its application in controlled release of Se-enriched supplements for Se-deficiency treatment.
33607408	6	41	theme	supplements	1219:1229	arg1	release					1196:1202	controlled release	1185:1202	controlled release of Se-enriched supplements	1185:1229	Additionally, EPS-SeNPs (Se/EPS = 1/20) showed a low Se release after SGF digestion, but a high release after SIF digestion, suggesting its application in controlled release of Se-enriched supplements for Se-deficiency treatment.
33607408	4	42	from	Se/EPS = 1/20	831:843	arg1	SIF					848:850	SIF	848:850	SIF	848:850	Se release kinetics from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SGF) followed a classical Fickian diffusion, in contrast to an erosion governed by macromolecular chains relaxation for Se/EPS = 1/20 in SIF.
33607408	6	43	theme	high	1121:1124	arg1	release					1126:1132	a high release	1119:1132	a high release	1119:1132	Additionally, EPS-SeNPs (Se/EPS = 1/20) showed a low Se release after SGF digestion, but a high release after SIF digestion, suggesting its application in controlled release of Se-enriched supplements for Se-deficiency treatment.
33607408	3	44	theme	Se	639:640	arg1	release					642:648	Se release	639:648	Se release	639:648	Se/EPS ratio and pH had significant influence on Se release.
33607408	4	45	dep	erosion	774:780	arg1	contrast					759:766	contrast	759:766	contrast	759:766	Se release kinetics from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SGF) followed a classical Fickian diffusion, in contrast to an erosion governed by macromolecular chains relaxation for Se/EPS = 1/20 in SIF.
33607408	6	46	theme	Se-enriched	1207:1217	arg1	supplements					1219:1229	Se-enriched supplements	1207:1229	Se-enriched supplements	1207:1229	Additionally, EPS-SeNPs (Se/EPS = 1/20) showed a low Se release after SGF digestion, but a high release after SIF digestion, suggesting its application in controlled release of Se-enriched supplements for Se-deficiency treatment.
33607408	4	47	theme	macromolecular	794:807	arg1	chains					809:814	macromolecular chains	794:814	macromolecular chains relaxation for Se/EPS = 1/20 in SIF	794:850	Se release kinetics from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SGF) followed a classical Fickian diffusion, in contrast to an erosion governed by macromolecular chains relaxation for Se/EPS = 1/20 in SIF.
33607408	1	48	from	nanoparticles	257:269	arg1	mechanism					215:223	mechanism	215:223	mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF)	215:364	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	1	48	from	nanoparticles	257:269	arg1	kinetics					202:209	selenium (Se) release kinetics	180:209	selenium (Se) release kinetics	180:209	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	1	48	from	nanoparticles	257:269	arg1	SIF					361:363	SIF	361:363	SIF	361:363	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	1	48	from	nanoparticles	257:269	arg1	fluids					353:358	simulated gastric (SGF) and intestinal fluids	314:358	simulated gastric (SGF) and intestinal fluids (SIF)	314:364	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	4	49	theme	Fickian	737:743	arg1	diffusion					745:753	a classical Fickian diffusion	725:753	a classical Fickian diffusion	725:753	Se release kinetics from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SGF) followed a classical Fickian diffusion, in contrast to an erosion governed by macromolecular chains relaxation for Se/EPS = 1/20 in SIF.
33607408	5	50	from	Se/EPS = 1/1	880:891	arg1	SIF					904:906	SIF	904:906	SIF	904:906	Se release from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SIF) was well-fitted to Korsmeyer-Peppas model and followed a non-Fickian mechanism controlled by both diffusion and erosion.
33607408	1	51	theme	selenium	180:187	arg1	kinetics					202:209	selenium (Se) release kinetics	180:209	selenium (Se) release kinetics	180:209	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	0	52	theme	composite	91:99	arg1	nanoparticles					101:113	Cordyceps sinensis exopolysaccharide-selenium composite nanoparticles	45:113	Cordyceps sinensis exopolysaccharide-selenium composite nanoparticles	45:113	Selenium release kinetics and mechanism from Cordyceps sinensis exopolysaccharide-selenium composite nanoparticles in simulated gastrointestinal conditions.
33607408	1	53	theme	Hixson-Crowell	425:438	arg1	models					381:386	kinetics models	372:386	kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas	372:459	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	4	54	theme	relaxation	816:825	arg1	chains					809:814	macromolecular chains	794:814	macromolecular chains relaxation for Se/EPS = 1/20 in SIF	794:850	Se release kinetics from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SGF) followed a classical Fickian diffusion, in contrast to an erosion governed by macromolecular chains relaxation for Se/EPS = 1/20 in SIF.
33607408	4	55	theme	release	654:660	arg1	kinetics					662:669	Se release kinetics	651:669	Se release kinetics from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SGF)	651:714	Se release kinetics from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SGF) followed a classical Fickian diffusion, in contrast to an erosion governed by macromolecular chains relaxation for Se/EPS = 1/20 in SIF.
33607408	0	56	theme	exopolysaccharide-selenium	64:89	arg1	nanoparticles					101:113	Cordyceps sinensis exopolysaccharide-selenium composite nanoparticles	45:113	Cordyceps sinensis exopolysaccharide-selenium composite nanoparticles	45:113	Selenium release kinetics and mechanism from Cordyceps sinensis exopolysaccharide-selenium composite nanoparticles in simulated gastrointestinal conditions.
33607408	1	57	dep	nanoparticles	257:269	arg1	nanoparticles					257:269	exopolysaccharide-selenium nanoparticles	230:269	exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF)	230:364	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	1	57	dep	nanoparticles	257:269	arg1	4/3					306:308	4/3	306:308	4/3	306:308	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	1	57	dep	nanoparticles	257:269	arg1	1/1					298:300	1/1	298:300	1/1	298:300	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	1	57	dep	nanoparticles	257:269	arg1	Se/EPS = 1/20					283:295	Se/EPS = 1/20	283:295	Se/EPS = 1/20	283:295	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	1	57	dep	nanoparticles	257:269	arg1	EPS-SeNPs					272:280	EPS-SeNPs	272:280	EPS-SeNPs	272:280	This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas.
33607408	5	58	from	EPS-SeNPs	869:877	arg1	release					856:862	Se release	853:862	Se release from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SIF)	853:907	Se release from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SIF) was well-fitted to Korsmeyer-Peppas model and followed a non-Fickian mechanism controlled by both diffusion and erosion.
33607408	3	59	dep	Se/EPS	590:595	arg1	Se/EPS					590:595	Se/EPS ratio and pH	590:608	Se/EPS ratio and pH	590:608	Se/EPS ratio and pH had significant influence on Se release.
33607408	3	59	dep	Se/EPS	590:595	arg1	pH					607:608	pH	607:608	pH	607:608	Se/EPS ratio and pH had significant influence on Se release.
33607408	3	59	dep	Se/EPS	590:595	arg1	ratio					597:601	ratio	597:601	ratio	597:601	Se/EPS ratio and pH had significant influence on Se release.
33254979	0	0	theme	temperature	108:118	arg1	processes					131:139	temperature controlled processes	108:139	temperature controlled processes	108:139	Efficient non-contact heat generation on flexible, ternary hydroxyapatite/curdlan/nanomagnetite hybrids for temperature controlled processes.
33254979	1	1	theme	808 nm	368:373	arg1	action					320:325	action	320:325	action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation	320:440	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	3	2	theme	composite	745:753	arg1	materials					755:763	composite materials	745:763	composite materials	745:763	Measurements were carried out on dry and Ringer's solution soaked composite materials in order to mimic in situ conditions.
33254979	1	3	theme	polymer	205:211	arg1	incorporation					219:231	polymer phase incorporation	205:231	polymer phase incorporation of magnetite	205:244	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	4	4	theme	energy	910:915	arg1	conversion					917:926	energy conversion	910:926	energy conversion emphasizing stability of the hybrids	910:963	It was found that the MNPs release during prolonged experiment is limited and has no significant effect on energy conversion emphasizing stability of the hybrids.
33254979	1	5	theme	heating	298:304	arg1	ability					306:312	their heating ability	292:312	their heating ability	292:312	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	2	6	theme	absorption	504:513	arg1	rate					515:518	the specific absorption rate	491:518	the specific absorption rate (SAR)	491:524	The energy conversion was evaluated in terms of the specific absorption rate (SAR) as a function of the MNPs concentration in composites and to estimate their potential in temperature-controlled regenerative processes and hyperthermia.
33254979	7	7	theme	curdlan	1418:1424	arg1	phase					1435:1439	the curdlan (polymer phase)	1414:1440	the curdlan (polymer phase)	1414:1440	MNPs incorporation into the curdlan (polymer phase) lead to strengthening of the mechanical properties of the whole network.
33254979	2	8	theme	rate	515:518	arg1	terms					482:486	terms	482:486	terms of the specific absorption rate (SAR)	482:524	The energy conversion was evaluated in terms of the specific absorption rate (SAR) as a function of the MNPs concentration in composites and to estimate their potential in temperature-controlled regenerative processes and hyperthermia.
33254979	1	9	theme	phase	213:217	arg1	incorporation					219:231	polymer phase incorporation	205:231	polymer phase incorporation of magnetite	205:244	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	4	10	contain	has	881:883	arg2	effect					900:905	no significant effect	885:905	no significant effect	885:905	It was found that the MNPs release during prolonged experiment is limited and has no significant effect on energy conversion emphasizing stability of the hybrids.
33254979	4	10	contain	has	881:883	arg1	release					830:836	the MNPs release	821:836	the MNPs release during prolonged experiment	821:864	It was found that the MNPs release during prolonged experiment is limited and has no significant effect on energy conversion emphasizing stability of the hybrids.
33254979	1	11	theme	infrared	380:387	arg1	radiation					395:403	infrared laser radiation	380:403	infrared laser radiation (NIR)	380:409	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	1	11	theme	infrared	380:387	arg1	NIR					406:408	NIR	406:408	NIR	406:408	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	6	12	theme	short	1265:1269	arg1	time					1271:1274	a relatively short time	1252:1274	a relatively short time of exposure to stimulating factors	1252:1309	In general, it was shown that maximum temperature of hybrid can be achieved in a relatively short time of exposure to stimulating factors whereas its control can be done through optimization of experiment conditions.
33254979	4	13	theme	prolonged	845:853	arg1	experiment					855:864	prolonged experiment	845:864	prolonged experiment	845:864	It was found that the MNPs release during prolonged experiment is limited and has no significant effect on energy conversion emphasizing stability of the hybrids.
33254979	5	14	theme	MNPs	987:990	arg1	Incorporation					966:978	Incorporation	966:978	Incorporation of the MNPs in polymer phase of the hybrid	966:1021	Incorporation of the MNPs in polymer phase of the hybrid can additionally limit particle leaking as well as plays a role as insulating layer for the heat dissipation lowering the risk of sample overheating.
33254979	5	15	theme	insulating	1090:1099	arg1	layer					1101:1105	insulating layer	1090:1105	insulating layer	1090:1105	Incorporation of the MNPs in polymer phase of the hybrid can additionally limit particle leaking as well as plays a role as insulating layer for the heat dissipation lowering the risk of sample overheating.
33254979	0	16	theme	controlled	120:129	arg1	processes					131:139	temperature controlled processes	108:139	temperature controlled processes	108:139	Efficient non-contact heat generation on flexible, ternary hydroxyapatite/curdlan/nanomagnetite hybrids for temperature controlled processes.
33254979	1	17	theme	laser	389:393	arg1	radiation					395:403	infrared laser radiation	380:403	infrared laser radiation (NIR)	380:409	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	1	17	theme	laser	389:393	arg1	NIR					406:408	NIR	406:408	NIR	406:408	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	4	18	theme	significant	888:898	arg1	effect					900:905	no significant effect	885:905	no significant effect	885:905	It was found that the MNPs release during prolonged experiment is limited and has no significant effect on energy conversion emphasizing stability of the hybrids.
33254979	7	19	theme	mechanical	1471:1480	arg1	properties					1482:1491	the mechanical properties	1467:1491	the mechanical properties of the whole network	1467:1512	MNPs incorporation into the curdlan (polymer phase) lead to strengthening of the mechanical properties of the whole network.
33254979	1	20	with	hybrids	180:186	arg1	MNPs					261:264	MNPs	261:264	MNPs	261:264	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	1	20	with	hybrids	180:186	arg1	nanoparticles					246:258	ceramic and polymer phase incorporation of magnetite nanoparticles	193:258	ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs)	193:265	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	5	21	theme	polymer	995:1001	arg1	phase					1003:1007	polymer phase	995:1007	polymer phase of the hybrid	995:1021	Incorporation of the MNPs in polymer phase of the hybrid can additionally limit particle leaking as well as plays a role as insulating layer for the heat dissipation lowering the risk of sample overheating.
33254979	1	22	theme	incorporation	219:231	arg1	MNPs					261:264	MNPs	261:264	MNPs	261:264	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	1	22	theme	incorporation	219:231	arg1	nanoparticles					246:258	ceramic and polymer phase incorporation of magnetite nanoparticles	193:258	ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs)	193:265	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	2	23	theme	specific	495:502	arg1	rate					515:518	the specific absorption rate	491:518	the specific absorption rate (SAR)	491:524	The energy conversion was evaluated in terms of the specific absorption rate (SAR) as a function of the MNPs concentration in composites and to estimate their potential in temperature-controlled regenerative processes and hyperthermia.
33254979	0	24	theme	non-contact	10:20	arg1	generation					27:36	Efficient non-contact heat generation	0:36	Efficient non-contact heat generation on flexible, ternary hydroxyapatite/curdlan/nanomagnetite hybrids for temperature controlled processes.	0:140	Efficient non-contact heat generation on flexible, ternary hydroxyapatite/curdlan/nanomagnetite hybrids for temperature controlled processes.
33254979	2	25	from	potential	602:610	arg1	hyperthermia					665:676	hyperthermia	665:676	hyperthermia	665:676	The energy conversion was evaluated in terms of the specific absorption rate (SAR) as a function of the MNPs concentration in composites and to estimate their potential in temperature-controlled regenerative processes and hyperthermia.
33254979	2	25	from	potential	602:610	arg1	processes					651:659	temperature-controlled regenerative processes	615:659	temperature-controlled regenerative processes	615:659	The energy conversion was evaluated in terms of the specific absorption rate (SAR) as a function of the MNPs concentration in composites and to estimate their potential in temperature-controlled regenerative processes and hyperthermia.
33254979	2	26	theme	regenerative	638:649	arg1	processes					651:659	temperature-controlled regenerative processes	615:659	temperature-controlled regenerative processes	615:659	The energy conversion was evaluated in terms of the specific absorption rate (SAR) as a function of the MNPs concentration in composites and to estimate their potential in temperature-controlled regenerative processes and hyperthermia.
33254979	6	27	theme	stimulating	1291:1301	arg1	factors					1303:1309	stimulating factors	1291:1309	stimulating factors	1291:1309	In general, it was shown that maximum temperature of hybrid can be achieved in a relatively short time of exposure to stimulating factors whereas its control can be done through optimization of experiment conditions.
33254979	1	28	theme	magnetite	236:244	arg1	incorporation					219:231	polymer phase incorporation	205:231	polymer phase incorporation of magnetite	205:244	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	1	28	theme	magnetite	236:244	arg1	ceramic					193:199	ceramic	193:199	ceramic	193:199	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	0	29	theme	Efficient	0:8	arg1	generation					27:36	Efficient non-contact heat generation	0:36	Efficient non-contact heat generation on flexible, ternary hydroxyapatite/curdlan/nanomagnetite hybrids for temperature controlled processes.	0:140	Efficient non-contact heat generation on flexible, ternary hydroxyapatite/curdlan/nanomagnetite hybrids for temperature controlled processes.
33254979	2	30	theme	temperature-controlled	615:636	arg1	processes					651:659	temperature-controlled regenerative processes	615:659	temperature-controlled regenerative processes	615:659	The energy conversion was evaluated in terms of the specific absorption rate (SAR) as a function of the MNPs concentration in composites and to estimate their potential in temperature-controlled regenerative processes and hyperthermia.
33254979	7	31	theme	properties	1482:1491	arg1	strengthening					1450:1462	strengthening	1450:1462	strengthening of the mechanical properties of the whole network	1450:1512	MNPs incorporation into the curdlan (polymer phase) lead to strengthening of the mechanical properties of the whole network.
33254979	7	32	theme	whole	1500:1504	arg1	network					1506:1512	the whole network	1496:1512	the whole network	1496:1512	MNPs incorporation into the curdlan (polymer phase) lead to strengthening of the mechanical properties of the whole network.
33254979	3	33	theme	in	783:784	arg1	conditions					791:800	in situ conditions	783:800	in situ conditions	783:800	Measurements were carried out on dry and Ringer's solution soaked composite materials in order to mimic in situ conditions.
33254979	2	34	theme	concentration	552:564	arg1	potential					602:610	their potential	596:610	their potential in temperature-controlled regenerative processes and hyperthermia	596:676	The energy conversion was evaluated in terms of the specific absorption rate (SAR) as a function of the MNPs concentration in composites and to estimate their potential in temperature-controlled regenerative processes and hyperthermia.
33254979	2	34	theme	concentration	552:564	arg1	conversion					454:463	The energy conversion	443:463	The energy conversion	443:463	The energy conversion was evaluated in terms of the specific absorption rate (SAR) as a function of the MNPs concentration in composites and to estimate their potential in temperature-controlled regenerative processes and hyperthermia.
33254979	2	34	theme	concentration	552:564	arg1	function					531:538	a function	529:538	a function of the MNPs concentration in composites	529:578	The energy conversion was evaluated in terms of the specific absorption rate (SAR) as a function of the MNPs concentration in composites and to estimate their potential in temperature-controlled regenerative processes and hyperthermia.
33254979	7	35	theme	MNPs	1390:1393	arg1	incorporation					1395:1407	MNPs incorporation	1390:1407	MNPs incorporation into the curdlan (polymer phase)	1390:1440	MNPs incorporation into the curdlan (polymer phase) lead to strengthening of the mechanical properties of the whole network.
33254979	2	36	theme	energy	447:452	arg1	potential					602:610	their potential	596:610	their potential in temperature-controlled regenerative processes and hyperthermia	596:676	The energy conversion was evaluated in terms of the specific absorption rate (SAR) as a function of the MNPs concentration in composites and to estimate their potential in temperature-controlled regenerative processes and hyperthermia.
33254979	2	36	theme	energy	447:452	arg1	conversion					454:463	The energy conversion	443:463	The energy conversion	443:463	The energy conversion was evaluated in terms of the specific absorption rate (SAR) as a function of the MNPs concentration in composites and to estimate their potential in temperature-controlled regenerative processes and hyperthermia.
33254979	2	36	theme	energy	447:452	arg1	function					531:538	a function	529:538	a function of the MNPs concentration in composites	529:578	The energy conversion was evaluated in terms of the specific absorption rate (SAR) as a function of the MNPs concentration in composites and to estimate their potential in temperature-controlled regenerative processes and hyperthermia.
33254979	0	37	theme	heat	22:25	arg1	generation					27:36	Efficient non-contact heat generation	0:36	Efficient non-contact heat generation on flexible, ternary hydroxyapatite/curdlan/nanomagnetite hybrids for temperature controlled processes.	0:140	Efficient non-contact heat generation on flexible, ternary hydroxyapatite/curdlan/nanomagnetite hybrids for temperature controlled processes.
33254979	1	38	theme	alternating	334:344	arg1	AMF					362:364	AMF	362:364	AMF	362:364	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	1	38	theme	alternating	334:344	arg1	field					355:359	the alternating magnetic field	330:359	the alternating magnetic field (AMF)	330:365	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	2	39	theme	MNPs	547:550	arg1	concentration					552:564	the MNPs concentration	543:564	the MNPs concentration in composites	543:578	The energy conversion was evaluated in terms of the specific absorption rate (SAR) as a function of the MNPs concentration in composites and to estimate their potential in temperature-controlled regenerative processes and hyperthermia.
33254979	0	40	theme	flexible	41:48	arg1	hybrids					96:102	flexible, ternary hydroxyapatite/curdlan/nanomagnetite hybrids	41:102	flexible, ternary hydroxyapatite/curdlan/nanomagnetite hybrids	41:102	Efficient non-contact heat generation on flexible, ternary hydroxyapatite/curdlan/nanomagnetite hybrids for temperature controlled processes.
33254979	1	41	theme	magnetic	346:353	arg1	AMF					362:364	AMF	362:364	AMF	362:364	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	1	41	theme	magnetic	346:353	arg1	field					355:359	the alternating magnetic field	330:359	the alternating magnetic field (AMF)	330:365	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	4	42	theme	hybrids	957:963	arg1	stability					940:948	stability	940:948	stability of the hybrids	940:963	It was found that the MNPs release during prolonged experiment is limited and has no significant effect on energy conversion emphasizing stability of the hybrids.
33254979	2	43	from	composites	569:578	arg1	potential					602:610	their potential	596:610	their potential in temperature-controlled regenerative processes and hyperthermia	596:676	The energy conversion was evaluated in terms of the specific absorption rate (SAR) as a function of the MNPs concentration in composites and to estimate their potential in temperature-controlled regenerative processes and hyperthermia.
33254979	2	43	from	composites	569:578	arg1	conversion					454:463	The energy conversion	443:463	The energy conversion	443:463	The energy conversion was evaluated in terms of the specific absorption rate (SAR) as a function of the MNPs concentration in composites and to estimate their potential in temperature-controlled regenerative processes and hyperthermia.
33254979	2	43	from	composites	569:578	arg1	function					531:538	a function	529:538	a function of the MNPs concentration in composites	529:578	The energy conversion was evaluated in terms of the specific absorption rate (SAR) as a function of the MNPs concentration in composites and to estimate their potential in temperature-controlled regenerative processes and hyperthermia.
33254979	1	44	theme	field	355:359	arg1	action					320:325	action	320:325	action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation	320:440	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	4	45	theme	MNPs	825:828	arg1	release					830:836	the MNPs release	821:836	the MNPs release during prolonged experiment	821:864	It was found that the MNPs release during prolonged experiment is limited and has no significant effect on energy conversion emphasizing stability of the hybrids.
33254979	5	46	theme	sample	1153:1158	arg1	overheating					1160:1170	sample overheating	1153:1170	sample overheating	1153:1170	Incorporation of the MNPs in polymer phase of the hybrid can additionally limit particle leaking as well as plays a role as insulating layer for the heat dissipation lowering the risk of sample overheating.
33254979	2	47	dep	rate	515:518	arg1	SAR					521:523	SAR	521:523	SAR	521:523	The energy conversion was evaluated in terms of the specific absorption rate (SAR) as a function of the MNPs concentration in composites and to estimate their potential in temperature-controlled regenerative processes and hyperthermia.
33254979	1	48	theme	synergic	421:428	arg1	stimulation					430:440	their synergic stimulation	415:440	their synergic stimulation	415:440	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	0	49	dep	flexible	41:48	arg1	ternary					51:57	ternary	51:57	ternary	51:57	Efficient non-contact heat generation on flexible, ternary hydroxyapatite/curdlan/nanomagnetite hybrids for temperature controlled processes.
33254979	1	50	theme	ternary	146:152	arg1	hybrids					180:186	The ternary HAp/curdlan/nanomagnetite hybrids	142:186	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs)	142:265	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	3	51	dep	in	783:784	arg1	situ					786:789	situ	786:789	situ	786:789	Measurements were carried out on dry and Ringer's solution soaked composite materials in order to mimic in situ conditions.
33254979	5	52	theme	overheating	1160:1170	arg1	risk					1145:1148	the risk	1141:1148	the risk of sample overheating	1141:1170	Incorporation of the MNPs in polymer phase of the hybrid can additionally limit particle leaking as well as plays a role as insulating layer for the heat dissipation lowering the risk of sample overheating.
33254979	5	53	theme	heat	1115:1118	arg1	dissipation					1120:1130	the heat dissipation	1111:1130	the heat dissipation lowering the risk of sample overheating	1111:1170	Incorporation of the MNPs in polymer phase of the hybrid can additionally limit particle leaking as well as plays a role as insulating layer for the heat dissipation lowering the risk of sample overheating.
33254979	1	54	theme	stimulation	430:440	arg1	action					320:325	action	320:325	action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation	320:440	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	7	55	theme	network	1506:1512	arg1	properties					1482:1491	the mechanical properties	1467:1491	the mechanical properties of the whole network	1467:1512	MNPs incorporation into the curdlan (polymer phase) lead to strengthening of the mechanical properties of the whole network.
33254979	1	56	theme	HAp/curdlan/nanomagnetite	154:178	arg1	hybrids					180:186	The ternary HAp/curdlan/nanomagnetite hybrids	142:186	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs)	142:265	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	6	57	theme	experiment	1367:1376	arg1	conditions					1378:1387	experiment conditions	1367:1387	experiment conditions	1367:1387	In general, it was shown that maximum temperature of hybrid can be achieved in a relatively short time of exposure to stimulating factors whereas its control can be done through optimization of experiment conditions.
33254979	6	58	theme	conditions	1378:1387	arg1	optimization					1351:1362	optimization	1351:1362	optimization of experiment conditions	1351:1387	In general, it was shown that maximum temperature of hybrid can be achieved in a relatively short time of exposure to stimulating factors whereas its control can be done through optimization of experiment conditions.
33254979	6	59	theme	maximum	1203:1209	arg1	temperature					1211:1221	maximum temperature	1203:1221	maximum temperature of hybrid	1203:1231	In general, it was shown that maximum temperature of hybrid can be achieved in a relatively short time of exposure to stimulating factors whereas its control can be done through optimization of experiment conditions.
33254979	6	60	theme	hybrid	1226:1231	arg1	temperature					1211:1221	maximum temperature	1203:1221	maximum temperature of hybrid	1203:1231	In general, it was shown that maximum temperature of hybrid can be achieved in a relatively short time of exposure to stimulating factors whereas its control can be done through optimization of experiment conditions.
33254979	6	61	theme	exposure	1279:1286	arg1	time					1271:1274	a relatively short time	1252:1274	a relatively short time of exposure to stimulating factors	1252:1309	In general, it was shown that maximum temperature of hybrid can be achieved in a relatively short time of exposure to stimulating factors whereas its control can be done through optimization of experiment conditions.
33254979	2	62	from	function	531:538	arg1	composites					569:578	composites	569:578	composites	569:578	The energy conversion was evaluated in terms of the specific absorption rate (SAR) as a function of the MNPs concentration in composites and to estimate their potential in temperature-controlled regenerative processes and hyperthermia.
33254979	7	63	theme	polymer	1427:1433	arg1	phase					1435:1439	the curdlan (polymer phase)	1414:1440	the curdlan (polymer phase)	1414:1440	MNPs incorporation into the curdlan (polymer phase) lead to strengthening of the mechanical properties of the whole network.
33254979	5	64	theme	hybrid	1016:1021	arg1	phase					1003:1007	polymer phase	995:1007	polymer phase of the hybrid	995:1021	Incorporation of the MNPs in polymer phase of the hybrid can additionally limit particle leaking as well as plays a role as insulating layer for the heat dissipation lowering the risk of sample overheating.
33254979	1	65	theme	ceramic	193:199	arg1	MNPs					261:264	MNPs	261:264	MNPs	261:264	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	1	65	theme	ceramic	193:199	arg1	nanoparticles					246:258	ceramic and polymer phase incorporation of magnetite nanoparticles	193:258	ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs)	193:265	The ternary HAp/curdlan/nanomagnetite hybrids with ceramic and polymer phase incorporation of magnetite nanoparticles (MNPs) were fabricated to study their heating ability under action of the alternating magnetic field (AMF), 808 nm near infrared laser radiation (NIR) and their synergic stimulation.
33254979	5	66	from	Incorporation	966:978	arg1	phase					1003:1007	polymer phase	995:1007	polymer phase of the hybrid	995:1021	Incorporation of the MNPs in polymer phase of the hybrid can additionally limit particle leaking as well as plays a role as insulating layer for the heat dissipation lowering the risk of sample overheating.
33254979	2	67	from	concentration	552:564	arg1	composites					569:578	composites	569:578	composites	569:578	The energy conversion was evaluated in terms of the specific absorption rate (SAR) as a function of the MNPs concentration in composites and to estimate their potential in temperature-controlled regenerative processes and hyperthermia.
33254979	0	68	theme	hydroxyapatite/curdlan/nanomagnetite	59:94	arg1	hybrids					96:102	flexible, ternary hydroxyapatite/curdlan/nanomagnetite hybrids	41:102	flexible, ternary hydroxyapatite/curdlan/nanomagnetite hybrids	41:102	Efficient non-contact heat generation on flexible, ternary hydroxyapatite/curdlan/nanomagnetite hybrids for temperature controlled processes.
33254979	0	69	from	generation	27:36	arg1	hybrids					96:102	flexible, ternary hydroxyapatite/curdlan/nanomagnetite hybrids	41:102	flexible, ternary hydroxyapatite/curdlan/nanomagnetite hybrids	41:102	Efficient non-contact heat generation on flexible, ternary hydroxyapatite/curdlan/nanomagnetite hybrids for temperature controlled processes.
33391325	13	0	theme	mass	2020:2023	arg1	analysis					2039:2046	mass spectrometric analysis	2020:2046	mass spectrometric analysis of a stably co-expressed recombinant human protein	2020:2097	Functional complex-type N-glycan sialylation was confirmed via mass spectrometric analysis of a stably co-expressed recombinant human protein.
33391325	11	1	theme	sialic	1685:1690	arg1	acid					1692:1695	free (Neu5Ac) and activated (CMP-Neu5Ac) sialic acid	1644:1695	free (Neu5Ac) and activated (CMP-Neu5Ac) sialic acid	1644:1695	Production of free (Neu5Ac) and activated (CMP-Neu5Ac) sialic acid was proven.
33391325	8	2	theme	N-glycan	1075:1082	arg1	sialylation					1084:1094	N-glycan sialylation	1075:1094	N-glycan sialylation	1075:1094	Since sialic acids are a common terminal modification on human N-glycans, the property to perform N-glycan sialylation is highly desired within the plant-based biopharmaceutical sector.
33391325	6	3	theme	biopharmaceutical	734:750	arg1	scalability					805:815	scalability	805:815	scalability	805:815	Furthermore, Physcomitrella meets wide-ranging biopharmaceutical requirements such as GMP compliance, product safety, scalability and outstanding possibilities for precise genome engineering.
33391325	6	3	theme	biopharmaceutical	734:750	arg1	possibilities					833:845	outstanding possibilities	821:845	outstanding possibilities	821:845	Furthermore, Physcomitrella meets wide-ranging biopharmaceutical requirements such as GMP compliance, product safety, scalability and outstanding possibilities for precise genome engineering.
33391325	6	3	theme	biopharmaceutical	734:750	arg1	safety					797:802	product safety	789:802	product safety	789:802	Furthermore, Physcomitrella meets wide-ranging biopharmaceutical requirements such as GMP compliance, product safety, scalability and outstanding possibilities for precise genome engineering.
33391325	6	3	theme	biopharmaceutical	734:750	arg1	compliance					777:786	GMP compliance	773:786	GMP compliance	773:786	Furthermore, Physcomitrella meets wide-ranging biopharmaceutical requirements such as GMP compliance, product safety, scalability and outstanding possibilities for precise genome engineering.
33391325	6	3	theme	biopharmaceutical	734:750	arg1	requirements					752:763	wide-ranging biopharmaceutical requirements	721:763	wide-ranging biopharmaceutical requirements such as GMP compliance, product safety, scalability and outstanding possibilities for precise genome engineering	721:876	Furthermore, Physcomitrella meets wide-ranging biopharmaceutical requirements such as GMP compliance, product safety, scalability and outstanding possibilities for precise genome engineering.
33391325	10	4	theme	Δxt/ft	1328:1333	arg1	line					1340:1343	a Δxt/ft moss line	1326:1343	a Δxt/ft moss line	1326:1343	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	12	5	theme	gene	1900:1903	arg1	knock-out					1883:1891	the simultaneous knock-out	1866:1891	the simultaneous knock-out of the gene encoding the endogenous β1,3-galactosyltransferase	1866:1954	The glycosidic anchor for the attachment of terminal sialic acid was generated by the introduction of a chimeric human β1,4-galactosyltransferase gene under the simultaneous knock-out of the gene encoding the endogenous β1,3-galactosyltransferase.
33391325	13	6	theme	spectrometric	2025:2037	arg1	analysis					2039:2046	mass spectrometric analysis	2020:2046	mass spectrometric analysis of a stably co-expressed recombinant human protein	2020:2097	Functional complex-type N-glycan sialylation was confirmed via mass spectrometric analysis of a stably co-expressed recombinant human protein.
33391325	12	7	theme	terminal	1753:1760	arg1	acid					1769:1772	terminal sialic acid	1753:1772	terminal sialic acid	1753:1772	The glycosidic anchor for the attachment of terminal sialic acid was generated by the introduction of a chimeric human β1,4-galactosyltransferase gene under the simultaneous knock-out of the gene encoding the endogenous β1,3-galactosyltransferase.
33391325	3	8	theme	therapeutical	286:298	arg1	applications					300:311	therapeutical applications	286:311	therapeutical applications	286:311	For therapeutical applications, a glycosylation pattern adapted to product and treatment requirements is advantageous.
33391325	4	9	theme	complex-type	483:494	arg1	N-glycosylation					496:510	highly homogeneous complex-type N-glycosylation	464:510	highly homogeneous complex-type N-glycosylation	464:510	Physcomitrium patens (Physcomitrella, moss) is able to perform highly homogeneous complex-type N-glycosylation.
33391325	2	10	theme	structural	248:257	arg1	properties					259:268	structural properties	248:268	structural properties	248:268	Since the majority of them are glycoproteins, their N-glycosylation profiles are major determinants for their activity, structural properties and safety.
33391325	12	11	theme	glycosidic	1713:1722	arg1	anchor					1724:1729	The glycosidic anchor	1709:1729	The glycosidic anchor for the attachment of terminal sialic acid	1709:1772	The glycosidic anchor for the attachment of terminal sialic acid was generated by the introduction of a chimeric human β1,4-galactosyltransferase gene under the simultaneous knock-out of the gene encoding the endogenous β1,3-galactosyltransferase.
33391325	10	12	theme	stable	1348:1353	arg1	expression					1355:1364	stable expression	1348:1364	stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid	1348:1627	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	5	13	theme	β1,2-xylosyltransferase	619:641	arg1	Δxt/ft					678:683	Δxt/ft	678:683	Δxt/ft	678:683	Additionally, it has been glyco-engineered to eliminate plant-specific sugar residues by knock-out of the β1,2-xylosyltransferase and α1,3-fucosyltransferase genes (Δxt/ft).
33391325	5	13	theme	β1,2-xylosyltransferase	619:641	arg1	genes					671:675	the β1,2-xylosyltransferase and α1,3-fucosyltransferase genes	615:675	the β1,2-xylosyltransferase and α1,3-fucosyltransferase genes (Δxt/ft)	615:684	Additionally, it has been glyco-engineered to eliminate plant-specific sugar residues by knock-out of the β1,2-xylosyltransferase and α1,3-fucosyltransferase genes (Δxt/ft).
33391325	3	14	theme	glycosylation	316:328	arg1	pattern					330:336	a glycosylation pattern	314:336	a glycosylation pattern adapted to product and treatment requirements	314:382	For therapeutical applications, a glycosylation pattern adapted to product and treatment requirements is advantageous.
33391325	3	14	theme	glycosylation	316:328	arg1	advantageous					387:398	advantageous	387:398	advantageous	387:398	For therapeutical applications, a glycosylation pattern adapted to product and treatment requirements is advantageous.
33391325	8	15	from	modification	1018:1029	arg1	N-glycans					1040:1048	human N-glycans	1034:1048	human N-glycans	1034:1048	Since sialic acids are a common terminal modification on human N-glycans, the property to perform N-glycan sialylation is highly desired within the plant-based biopharmaceutical sector.
33391325	5	16	theme	sugar	584:588	arg1	residues					590:597	plant-specific sugar residues	569:597	plant-specific sugar residues	569:597	Additionally, it has been glyco-engineered to eliminate plant-specific sugar residues by knock-out of the β1,2-xylosyltransferase and α1,3-fucosyltransferase genes (Δxt/ft).
33391325	10	17	theme	encoded	1464:1470	arg1	enzymes					1472:1478	the encoded enzymes	1460:1478	the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans	1460:1551	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	11	18	theme	free	1644:1647	arg1	acid					1692:1695	free (Neu5Ac) and activated (CMP-Neu5Ac) sialic acid	1644:1695	free (Neu5Ac) and activated (CMP-Neu5Ac) sialic acid	1644:1695	Production of free (Neu5Ac) and activated (CMP-Neu5Ac) sialic acid was proven.
33391325	12	19	theme	human	1822:1826	arg1	gene					1855:1858	a chimeric human β1,4-galactosyltransferase gene	1811:1858	a chimeric human β1,4-galactosyltransferase gene	1811:1858	The glycosidic anchor for the attachment of terminal sialic acid was generated by the introduction of a chimeric human β1,4-galactosyltransferase gene under the simultaneous knock-out of the gene encoding the endogenous β1,3-galactosyltransferase.
33391325	13	20	theme	complex-type	1968:1979	arg1	sialylation					1990:2000	Functional complex-type N-glycan sialylation	1957:2000	Functional complex-type N-glycan sialylation	1957:2000	Functional complex-type N-glycan sialylation was confirmed via mass spectrometric analysis of a stably co-expressed recombinant human protein.
33391325	6	21	theme	wide-ranging	721:732	arg1	scalability					805:815	scalability	805:815	scalability	805:815	Furthermore, Physcomitrella meets wide-ranging biopharmaceutical requirements such as GMP compliance, product safety, scalability and outstanding possibilities for precise genome engineering.
33391325	6	21	theme	wide-ranging	721:732	arg1	possibilities					833:845	outstanding possibilities	821:845	outstanding possibilities	821:845	Furthermore, Physcomitrella meets wide-ranging biopharmaceutical requirements such as GMP compliance, product safety, scalability and outstanding possibilities for precise genome engineering.
33391325	6	21	theme	wide-ranging	721:732	arg1	safety					797:802	product safety	789:802	product safety	789:802	Furthermore, Physcomitrella meets wide-ranging biopharmaceutical requirements such as GMP compliance, product safety, scalability and outstanding possibilities for precise genome engineering.
33391325	6	21	theme	wide-ranging	721:732	arg1	compliance					777:786	GMP compliance	773:786	GMP compliance	773:786	Furthermore, Physcomitrella meets wide-ranging biopharmaceutical requirements such as GMP compliance, product safety, scalability and outstanding possibilities for precise genome engineering.
33391325	6	21	theme	wide-ranging	721:732	arg1	requirements					752:763	wide-ranging biopharmaceutical requirements	721:763	wide-ranging biopharmaceutical requirements such as GMP compliance, product safety, scalability and outstanding possibilities for precise genome engineering	721:876	Furthermore, Physcomitrella meets wide-ranging biopharmaceutical requirements such as GMP compliance, product safety, scalability and outstanding possibilities for precise genome engineering.
33391325	8	22	theme	biopharmaceutical	1137:1153	arg1	sector					1155:1160	the plant-based biopharmaceutical sector	1121:1160	the plant-based biopharmaceutical sector	1121:1160	Since sialic acids are a common terminal modification on human N-glycans, the property to perform N-glycan sialylation is highly desired within the plant-based biopharmaceutical sector.
33391325	12	23	theme	gene	1855:1858	arg1	introduction					1795:1806	the introduction	1791:1806	the introduction of a chimeric human β1,4-galactosyltransferase gene under the simultaneous knock-out of the gene encoding the endogenous β1,3-galactosyltransferase	1791:1954	The glycosidic anchor for the attachment of terminal sialic acid was generated by the introduction of a chimeric human β1,4-galactosyltransferase gene under the simultaneous knock-out of the gene encoding the endogenous β1,3-galactosyltransferase.
33391325	11	24	dep	free	1644:1647	arg1	Neu5Ac					1650:1655	Neu5Ac	1650:1655	Neu5Ac	1650:1655	Production of free (Neu5Ac) and activated (CMP-Neu5Ac) sialic acid was proven.
33391325	10	25	theme	mammalian	1375:1383	arg1	sequences					1392:1400	seven mammalian coding sequences	1369:1400	seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid	1369:1627	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	8	26	theme	sialic	983:988	arg1	acids					990:994	sialic acids	983:994	sialic acids	983:994	Since sialic acids are a common terminal modification on human N-glycans, the property to perform N-glycan sialylation is highly desired within the plant-based biopharmaceutical sector.
33391325	8	26	theme	sialic	983:988	arg1	modification					1018:1029	a common terminal modification	1000:1029	a common terminal modification on human N-glycans	1000:1048	Since sialic acids are a common terminal modification on human N-glycans, the property to perform N-glycan sialylation is highly desired within the plant-based biopharmaceutical sector.
33391325	10	27	theme	organelle-specific	1425:1442	arg1	localization					1444:1455	targeted organelle-specific localization	1416:1455	targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid	1416:1627	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	13	28	theme	recombinant	2073:2083	arg1	protein					2091:2097	a stably co-expressed recombinant human protein	2051:2097	a stably co-expressed recombinant human protein	2051:2097	Functional complex-type N-glycan sialylation was confirmed via mass spectrometric analysis of a stably co-expressed recombinant human protein.
33391325	5	29	theme	α1,3-fucosyltransferase	647:669	arg1	Δxt/ft					678:683	Δxt/ft	678:683	Δxt/ft	678:683	Additionally, it has been glyco-engineered to eliminate plant-specific sugar residues by knock-out of the β1,2-xylosyltransferase and α1,3-fucosyltransferase genes (Δxt/ft).
33391325	5	29	theme	α1,3-fucosyltransferase	647:669	arg1	genes					671:675	the β1,2-xylosyltransferase and α1,3-fucosyltransferase genes	615:675	the β1,2-xylosyltransferase and α1,3-fucosyltransferase genes (Δxt/ft)	615:684	Additionally, it has been glyco-engineered to eliminate plant-specific sugar residues by knock-out of the β1,2-xylosyltransferase and α1,3-fucosyltransferase genes (Δxt/ft).
33391325	10	30	theme	acid	1624:1627	arg1	transfer					1605:1612	transfer	1605:1612	transfer	1605:1612	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	10	30	theme	acid	1624:1627	arg1	transport					1591:1599	transport	1591:1599	transport	1591:1599	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	10	30	theme	acid	1624:1627	arg1	localization					1444:1455	targeted organelle-specific localization	1416:1455	targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid	1416:1627	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	10	30	theme	acid	1624:1627	arg1	activation					1579:1588	activation	1579:1588	activation	1579:1588	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	10	30	theme	acid	1624:1627	arg1	synthesis					1568:1576	the synthesis	1564:1576	the synthesis	1564:1576	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	13	31	theme	human	2085:2089	arg1	protein					2091:2097	a stably co-expressed recombinant human protein	2051:2097	a stably co-expressed recombinant human protein	2051:2097	Functional complex-type N-glycan sialylation was confirmed via mass spectrometric analysis of a stably co-expressed recombinant human protein.
33391325	0	32	theme	Stable	0:5	arg1	Sialylation					15:25	Stable Protein Sialylation	0:25	Stable Protein Sialylation	0:25	Stable Protein Sialylation in Physcomitrella.
33391325	1	33	theme	medical	106:112	arg1	applications					114:125	medical applications	106:125	medical applications	106:125	Recombinantly produced proteins are indispensable tools for medical applications.
33391325	8	34	theme	common	1002:1007	arg1	acids					990:994	sialic acids	983:994	sialic acids	983:994	Since sialic acids are a common terminal modification on human N-glycans, the property to perform N-glycan sialylation is highly desired within the plant-based biopharmaceutical sector.
33391325	8	34	theme	common	1002:1007	arg1	modification					1018:1029	a common terminal modification	1000:1029	a common terminal modification on human N-glycans	1000:1048	Since sialic acids are a common terminal modification on human N-glycans, the property to perform N-glycan sialylation is highly desired within the plant-based biopharmaceutical sector.
33391325	3	35	dep	product	349:355	arg1	requirements					371:382	requirements	371:382	requirements	371:382	For therapeutical applications, a glycosylation pattern adapted to product and treatment requirements is advantageous.
33391325	6	36	theme	product	789:795	arg1	safety					797:802	product safety	789:802	product safety	789:802	Furthermore, Physcomitrella meets wide-ranging biopharmaceutical requirements such as GMP compliance, product safety, scalability and outstanding possibilities for precise genome engineering.
33391325	9	37	theme	sialylation	1236:1246	arg1	achievement					1204:1214	the successful achievement	1189:1214	the successful achievement of protein N-glycan sialylation in stably transformed Physcomitrella	1189:1283	In this study, we present the successful achievement of protein N-glycan sialylation in stably transformed Physcomitrella.
33391325	8	38	theme	human	1034:1038	arg1	N-glycans					1040:1048	human N-glycans	1034:1048	human N-glycans	1034:1048	Since sialic acids are a common terminal modification on human N-glycans, the property to perform N-glycan sialylation is highly desired within the plant-based biopharmaceutical sector.
33391325	10	39	theme	responsible	1480:1490	arg1	enzymes					1472:1478	the encoded enzymes	1460:1478	the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans	1460:1551	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	0	40	dep	Sialylation	15:25	arg1	Physcomitrella					30:43	Physcomitrella	30:43	Physcomitrella	30:43	Stable Protein Sialylation in Physcomitrella.
33391325	2	41	theme	major	209:213	arg1	profiles					196:203	their N-glycosylation profiles	174:203	their N-glycosylation profiles	174:203	Since the majority of them are glycoproteins, their N-glycosylation profiles are major determinants for their activity, structural properties and safety.
33391325	2	41	theme	major	209:213	arg1	determinants					215:226	major determinants	209:226	major determinants for their activity, structural properties and safety	209:279	Since the majority of them are glycoproteins, their N-glycosylation profiles are major determinants for their activity, structural properties and safety.
33391325	10	42	theme	acceptor	1534:1541	arg1	N-glycans					1543:1551	β1,4-galactosylated acceptor N-glycans	1514:1551	β1,4-galactosylated acceptor N-glycans	1514:1551	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	1	43	theme	produced	60:67	arg1	proteins					69:76	Recombinantly produced proteins	46:76	Recombinantly produced proteins	46:76	Recombinantly produced proteins are indispensable tools for medical applications.
33391325	1	43	theme	produced	60:67	arg1	tools					96:100	indispensable tools	82:100	indispensable tools for medical applications	82:125	Recombinantly produced proteins are indispensable tools for medical applications.
33391325	13	44	theme	protein	2091:2097	arg1	analysis					2039:2046	mass spectrometric analysis	2020:2046	mass spectrometric analysis of a stably co-expressed recombinant human protein	2020:2097	Functional complex-type N-glycan sialylation was confirmed via mass spectrometric analysis of a stably co-expressed recombinant human protein.
33391325	7	45	dep	mammals	915:921	arg1	contrast					903:910	contrast	903:910	contrast	903:910	However, all plants, in contrast to mammals, lack the capability to perform N-glycan sialylation.
33391325	12	46	theme	endogenous	1918:1927	arg1	β1,3-galactosyltransferase					1929:1954	the endogenous β1,3-galactosyltransferase	1914:1954	the endogenous β1,3-galactosyltransferase	1914:1954	The glycosidic anchor for the attachment of terminal sialic acid was generated by the introduction of a chimeric human β1,4-galactosyltransferase gene under the simultaneous knock-out of the gene encoding the endogenous β1,3-galactosyltransferase.
33391325	13	47	theme	Functional	1957:1966	arg1	sialylation					1990:2000	Functional complex-type N-glycan sialylation	1957:2000	Functional complex-type N-glycan sialylation	1957:2000	Functional complex-type N-glycan sialylation was confirmed via mass spectrometric analysis of a stably co-expressed recombinant human protein.
33391325	11	48	theme	acid	1692:1695	arg1	Production					1630:1639	Production	1630:1639	Production of free (Neu5Ac) and activated (CMP-Neu5Ac) sialic acid	1630:1695	Production of free (Neu5Ac) and activated (CMP-Neu5Ac) sialic acid was proven.
33391325	10	49	theme	moss	1335:1338	arg1	line					1340:1343	a Δxt/ft moss line	1326:1343	a Δxt/ft moss line	1326:1343	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	9	50	theme	protein	1219:1225	arg1	sialylation					1236:1246	protein N-glycan sialylation	1219:1246	protein N-glycan sialylation	1219:1246	In this study, we present the successful achievement of protein N-glycan sialylation in stably transformed Physcomitrella.
33391325	5	51	theme	plant-specific	569:582	arg1	residues					590:597	plant-specific sugar residues	569:597	plant-specific sugar residues	569:597	Additionally, it has been glyco-engineered to eliminate plant-specific sugar residues by knock-out of the β1,2-xylosyltransferase and α1,3-fucosyltransferase genes (Δxt/ft).
33391325	13	52	theme	N-glycan	1981:1988	arg1	sialylation					1990:2000	Functional complex-type N-glycan sialylation	1957:2000	Functional complex-type N-glycan sialylation	1957:2000	Functional complex-type N-glycan sialylation was confirmed via mass spectrometric analysis of a stably co-expressed recombinant human protein.
33391325	10	53	theme	sequences	1392:1400	arg1	expression					1355:1364	stable expression	1348:1364	stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid	1348:1627	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	2	54	theme	them	150:153	arg1	majority					138:145	the majority	134:145	the majority of them	134:153	Since the majority of them are glycoproteins, their N-glycosylation profiles are major determinants for their activity, structural properties and safety.
33391325	2	54	theme	them	150:153	arg1	glycoproteins					159:171	glycoproteins	159:171	glycoproteins	159:171	Since the majority of them are glycoproteins, their N-glycosylation profiles are major determinants for their activity, structural properties and safety.
33391325	7	55	theme	N-glycan	955:962	arg1	sialylation					964:974	N-glycan sialylation	955:974	N-glycan sialylation	955:974	However, all plants, in contrast to mammals, lack the capability to perform N-glycan sialylation.
33391325	8	56	theme	plant-based	1125:1135	arg1	sector					1155:1160	the plant-based biopharmaceutical sector	1121:1160	the plant-based biopharmaceutical sector	1121:1160	Since sialic acids are a common terminal modification on human N-glycans, the property to perform N-glycan sialylation is highly desired within the plant-based biopharmaceutical sector.
33391325	12	57	theme	sialic	1762:1767	arg1	acid					1769:1772	terminal sialic acid	1753:1772	terminal sialic acid	1753:1772	The glycosidic anchor for the attachment of terminal sialic acid was generated by the introduction of a chimeric human β1,4-galactosyltransferase gene under the simultaneous knock-out of the gene encoding the endogenous β1,3-galactosyltransferase.
33391325	6	58	theme	GMP	773:775	arg1	compliance					777:786	GMP compliance	773:786	GMP compliance	773:786	Furthermore, Physcomitrella meets wide-ranging biopharmaceutical requirements such as GMP compliance, product safety, scalability and outstanding possibilities for precise genome engineering.
33391325	4	59	theme	homogeneous	471:481	arg1	N-glycosylation					496:510	highly homogeneous complex-type N-glycosylation	464:510	highly homogeneous complex-type N-glycosylation	464:510	Physcomitrium patens (Physcomitrella, moss) is able to perform highly homogeneous complex-type N-glycosylation.
33391325	4	60	dep	moss	439:442	arg1	Physcomitrella					423:436	Physcomitrella	423:436	Physcomitrella	423:436	Physcomitrium patens (Physcomitrella, moss) is able to perform highly homogeneous complex-type N-glycosylation.
33391325	12	61	theme	simultaneous	1870:1881	arg1	knock-out					1883:1891	the simultaneous knock-out	1866:1891	the simultaneous knock-out of the gene encoding the endogenous β1,3-galactosyltransferase	1866:1954	The glycosidic anchor for the attachment of terminal sialic acid was generated by the introduction of a chimeric human β1,4-galactosyltransferase gene under the simultaneous knock-out of the gene encoding the endogenous β1,3-galactosyltransferase.
33391325	11	62	theme	activated	1662:1670	arg1	acid					1692:1695	free (Neu5Ac) and activated (CMP-Neu5Ac) sialic acid	1644:1695	free (Neu5Ac) and activated (CMP-Neu5Ac) sialic acid	1644:1695	Production of free (Neu5Ac) and activated (CMP-Neu5Ac) sialic acid was proven.
33391325	9	63	attach	present	1181:1187	arg2	we					1178:1179	we	1178:1179	we	1178:1179	In this study, we present the successful achievement of protein N-glycan sialylation in stably transformed Physcomitrella.
33391325	9	63	attach	present	1181:1187	arg1	study					1171:1175	this study	1166:1175	this study	1166:1175	In this study, we present the successful achievement of protein N-glycan sialylation in stably transformed Physcomitrella.
33391325	12	64	theme	chimeric	1813:1820	arg1	gene					1855:1858	a chimeric human β1,4-galactosyltransferase gene	1811:1858	a chimeric human β1,4-galactosyltransferase gene	1811:1858	The glycosidic anchor for the attachment of terminal sialic acid was generated by the introduction of a chimeric human β1,4-galactosyltransferase gene under the simultaneous knock-out of the gene encoding the endogenous β1,3-galactosyltransferase.
33391325	10	65	theme	coding	1385:1390	arg1	sequences					1392:1400	seven mammalian coding sequences	1369:1400	seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid	1369:1627	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	12	66	theme	β1,4-galactosyltransferase	1828:1853	arg1	gene					1855:1858	a chimeric human β1,4-galactosyltransferase gene	1811:1858	a chimeric human β1,4-galactosyltransferase gene	1811:1858	The glycosidic anchor for the attachment of terminal sialic acid was generated by the introduction of a chimeric human β1,4-galactosyltransferase gene under the simultaneous knock-out of the gene encoding the endogenous β1,3-galactosyltransferase.
33391325	10	67	theme	N-glycans	1543:1551	arg1	generation					1500:1509	the generation	1496:1509	the generation of β1,4-galactosylated acceptor N-glycans	1496:1551	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	2	68	gly	glycoproteins	159:171	arg1	majority					138:145	the majority	134:145	the majority of them	134:153	Since the majority of them are glycoproteins, their N-glycosylation profiles are major determinants for their activity, structural properties and safety.
33391325	2	68	gly	glycoproteins	159:171	arg1	glycoproteins					159:171	glycoproteins	159:171	glycoproteins	159:171	Since the majority of them are glycoproteins, their N-glycosylation profiles are major determinants for their activity, structural properties and safety.
33391325	6	69	theme	outstanding	821:831	arg1	possibilities					833:845	outstanding possibilities	821:845	outstanding possibilities	821:845	Furthermore, Physcomitrella meets wide-ranging biopharmaceutical requirements such as GMP compliance, product safety, scalability and outstanding possibilities for precise genome engineering.
33391325	1	70	theme	indispensable	82:94	arg1	proteins					69:76	Recombinantly produced proteins	46:76	Recombinantly produced proteins	46:76	Recombinantly produced proteins are indispensable tools for medical applications.
33391325	1	70	theme	indispensable	82:94	arg1	tools					96:100	indispensable tools	82:100	indispensable tools for medical applications	82:125	Recombinantly produced proteins are indispensable tools for medical applications.
33391325	9	71	theme	successful	1193:1202	arg1	achievement					1204:1214	the successful achievement	1189:1214	the successful achievement of protein N-glycan sialylation in stably transformed Physcomitrella	1189:1283	In this study, we present the successful achievement of protein N-glycan sialylation in stably transformed Physcomitrella.
33391325	0	72	theme	Protein	7:13	arg1	Sialylation					15:25	Stable Protein Sialylation	0:25	Stable Protein Sialylation	0:25	Stable Protein Sialylation in Physcomitrella.
33391325	5	73	theme	genes	671:675	arg1	knock-out					602:610	knock-out	602:610	knock-out of the β1,2-xylosyltransferase and α1,3-fucosyltransferase genes (Δxt/ft)	602:684	Additionally, it has been glyco-engineered to eliminate plant-specific sugar residues by knock-out of the β1,2-xylosyltransferase and α1,3-fucosyltransferase genes (Δxt/ft).
33391325	10	74	theme	sialic	1617:1622	arg1	acid					1624:1627	sialic acid	1617:1627	sialic acid	1617:1627	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	11	75	dep	activated	1662:1670	arg1	CMP-Neu5Ac					1673:1682	CMP-Neu5Ac	1673:1682	CMP-Neu5Ac	1673:1682	Production of free (Neu5Ac) and activated (CMP-Neu5Ac) sialic acid was proven.
33391325	10	76	theme	enzymes	1472:1478	arg1	transfer					1605:1612	transfer	1605:1612	transfer	1605:1612	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	10	76	theme	enzymes	1472:1478	arg1	transport					1591:1599	transport	1591:1599	transport	1591:1599	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	10	76	theme	enzymes	1472:1478	arg1	localization					1444:1455	targeted organelle-specific localization	1416:1455	targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid	1416:1627	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	10	76	theme	enzymes	1472:1478	arg1	activation					1579:1588	activation	1579:1588	activation	1579:1588	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	10	76	theme	enzymes	1472:1478	arg1	synthesis					1568:1576	the synthesis	1564:1576	the synthesis	1564:1576	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	12	77	theme	acid	1769:1772	arg1	attachment					1739:1748	the attachment	1735:1748	the attachment of terminal sialic acid	1735:1772	The glycosidic anchor for the attachment of terminal sialic acid was generated by the introduction of a chimeric human β1,4-galactosyltransferase gene under the simultaneous knock-out of the gene encoding the endogenous β1,3-galactosyltransferase.
33391325	6	78	theme	precise	851:857	arg1	engineering					866:876	precise genome engineering	851:876	precise genome engineering	851:876	Furthermore, Physcomitrella meets wide-ranging biopharmaceutical requirements such as GMP compliance, product safety, scalability and outstanding possibilities for precise genome engineering.
33391325	9	79	theme	N-glycan	1227:1234	arg1	sialylation					1236:1246	protein N-glycan sialylation	1219:1246	protein N-glycan sialylation	1219:1246	In this study, we present the successful achievement of protein N-glycan sialylation in stably transformed Physcomitrella.
33391325	13	80	theme	co-expressed	2060:2071	arg1	protein					2091:2097	a stably co-expressed recombinant human protein	2051:2097	a stably co-expressed recombinant human protein	2051:2097	Functional complex-type N-glycan sialylation was confirmed via mass spectrometric analysis of a stably co-expressed recombinant human protein.
33391325	8	81	theme	terminal	1009:1016	arg1	acids					990:994	sialic acids	983:994	sialic acids	983:994	Since sialic acids are a common terminal modification on human N-glycans, the property to perform N-glycan sialylation is highly desired within the plant-based biopharmaceutical sector.
33391325	8	81	theme	terminal	1009:1016	arg1	modification					1018:1029	a common terminal modification	1000:1029	a common terminal modification on human N-glycans	1000:1048	Since sialic acids are a common terminal modification on human N-glycans, the property to perform N-glycan sialylation is highly desired within the plant-based biopharmaceutical sector.
33391325	2	82	theme	N-glycosylation	180:194	arg1	profiles					196:203	their N-glycosylation profiles	174:203	their N-glycosylation profiles	174:203	Since the majority of them are glycoproteins, their N-glycosylation profiles are major determinants for their activity, structural properties and safety.
33391325	2	82	theme	N-glycosylation	180:194	arg1	determinants					215:226	major determinants	209:226	major determinants for their activity, structural properties and safety	209:279	Since the majority of them are glycoproteins, their N-glycosylation profiles are major determinants for their activity, structural properties and safety.
33391325	10	83	theme	sialylation	1290:1300	arg1	ability					1302:1308	The sialylation ability	1286:1308	The sialylation ability	1286:1308	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	9	84	theme	transformed	1258:1268	arg1	Physcomitrella					1270:1283	stably transformed Physcomitrella	1251:1283	stably transformed Physcomitrella	1251:1283	In this study, we present the successful achievement of protein N-glycan sialylation in stably transformed Physcomitrella.
33391325	9	85	from	achievement	1204:1214	arg1	Physcomitrella					1270:1283	stably transformed Physcomitrella	1251:1283	stably transformed Physcomitrella	1251:1283	In this study, we present the successful achievement of protein N-glycan sialylation in stably transformed Physcomitrella.
33391325	6	86	theme	genome	859:864	arg1	engineering					866:876	precise genome engineering	851:876	precise genome engineering	851:876	Furthermore, Physcomitrella meets wide-ranging biopharmaceutical requirements such as GMP compliance, product safety, scalability and outstanding possibilities for precise genome engineering.
33391325	10	87	theme	targeted	1416:1423	arg1	localization					1444:1455	targeted organelle-specific localization	1416:1455	targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid	1416:1627	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33391325	10	88	theme	β1,4-galactosylated	1514:1532	arg1	N-glycans					1543:1551	β1,4-galactosylated acceptor N-glycans	1514:1551	β1,4-galactosylated acceptor N-glycans	1514:1551	The sialylation ability was achieved in a Δxt/ft moss line by stable expression of seven mammalian coding sequences combined with targeted organelle-specific localization of the encoded enzymes responsible for the generation of β1,4-galactosylated acceptor N-glycans as well as the synthesis, activation, transport and transfer of sialic acid.
33533623	6	0	theme	nanometer-thick	998:1012	arg1	plates					1034:1039	nanometer-thick and micrometer-long plates	998:1039	nanometer-thick and micrometer-long plates	998:1039	In both surfaces, epicuticular wax is organized along a randomly oriented and intricate network of nanometer-thick and micrometer-long plates, whose density and degree of interconnection are significantly higher in the abaxial surface.
33533623	13	1	theme	siloxane-based	2482:2495	arg1	materials					2504:2512	1-octacosanol-grafted siloxane-based hybrid materials	2460:2512	1-octacosanol-grafted siloxane-based hybrid materials	2460:2512	Because high topographical variations of the biotemplate and wax adhesion to the biohybrid film affected the replication quality, the reproduction of the wax texture via the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials is proposed as a suitable route to duplicate the abaxial surface with high fidelity.
33533623	2	2	theme	naval	296:300	arg1	sectors					340:346	the aeronautical, space, naval, building, automobile, and biomedical sectors	271:346	the aeronautical, space, naval, building, automobile, and biomedical sectors	271:346	Such coatings hold a bright technological future in niche markets of the aeronautical, space, naval, building, automobile, and biomedical sectors.
33533623	10	3	theme	surface	1890:1896	arg1	dry					1898:1900	the surface dry	1886:1900	the surface dry	1886:1900	We show that the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet works as a sophisticated light and water management system: it reflects solar radiation diffusely to lower the surface temperature, and it has superhydrophobic character to keep the surface dry.
33533623	13	4	theme	materials	2504:2512	arg1	synthesis					2447:2455	the synthesis	2443:2455	the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials	2443:2512	Because high topographical variations of the biotemplate and wax adhesion to the biohybrid film affected the replication quality, the reproduction of the wax texture via the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials is proposed as a suitable route to duplicate the abaxial surface with high fidelity.
33533623	3	5	theme	Ceratonia	443:451	arg1	L					461:461	the Ceratonia silique L	439:461	the Ceratonia silique L. (carob)	439:470	This work is focused on the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world.
33533623	3	5	theme	Ceratonia	443:451	arg1	carob					465:469	carob	465:469	carob	465:469	This work is focused on the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world.
33533623	3	5	theme	Ceratonia	443:451	arg1	tree					511:514	a high-commercial-value Mediterranean tree	473:514	a high-commercial-value Mediterranean tree cultivated in many regions of the world	473:554	This work is focused on the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world.
33533623	14	6	theme	material	2814:2821	arg1	systems					2823:2829	diffuse reflecting and superhydrophobic material systems	2774:2829	diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings	2774:2914	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	10	7	contain	has	1847:1849	arg1	it					1844:1845	it	1844:1845	it	1844:1845	We show that the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet works as a sophisticated light and water management system: it reflects solar radiation diffusely to lower the surface temperature, and it has superhydrophobic character to keep the surface dry.
33533623	10	7	contain	has	1847:1849	arg2	character					1868:1876	superhydrophobic character	1851:1876	superhydrophobic character to keep the surface dry	1851:1900	We show that the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet works as a sophisticated light and water management system: it reflects solar radiation diffusely to lower the surface temperature, and it has superhydrophobic character to keep the surface dry.
33533623	10	8	theme	siliqua	1692:1698	arg1	leaflet					1700:1706	the C. siliqua leaflet	1685:1706	the C. siliqua leaflet	1685:1706	We show that the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet works as a sophisticated light and water management system: it reflects solar radiation diffusely to lower the surface temperature, and it has superhydrophobic character to keep the surface dry.
33533623	6	9	theme	interconnection	1070:1084	arg1	degree					1060:1065	degree	1060:1065	degree	1060:1065	In both surfaces, epicuticular wax is organized along a randomly oriented and intricate network of nanometer-thick and micrometer-long plates, whose density and degree of interconnection are significantly higher in the abaxial surface.
33533623	6	9	theme	interconnection	1070:1084	arg1	density					1048:1054	density	1048:1054	density	1048:1054	In both surfaces, epicuticular wax is organized along a randomly oriented and intricate network of nanometer-thick and micrometer-long plates, whose density and degree of interconnection are significantly higher in the abaxial surface.
33533623	5	10	theme	wax	791:793	arg1	layers					795:800	the epicuticular and intracuticular wax layers	755:800	the epicuticular and intracuticular wax layers of the adaxial surface	755:823	Their chemical composition is very simple: monopalmitin ester and palmitic acid are protuberant in the epicuticular and intracuticular wax layers of the adaxial surface, respectively, whereas 1-octacosanol dominates in the abaxial wax layers.
33533623	14	11	theme	diffuse	2774:2780	arg1	systems					2823:2829	diffuse reflecting and superhydrophobic material systems	2774:2829	diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings	2774:2914	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	14	12	theme	water-repelling	2891:2905	arg1	coatings					2907:2914	dual-functional antiglare and water-repelling coatings	2861:2914	dual-functional antiglare and water-repelling coatings	2861:2914	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	2	13	theme	niche	254:258	arg1	markets					260:266	niche markets	254:266	niche markets of the aeronautical, space, naval, building, automobile, and biomedical sectors	254:346	Such coatings hold a bright technological future in niche markets of the aeronautical, space, naval, building, automobile, and biomedical sectors.
33533623	1	14	theme	plant	166:170	arg1	surface					155:161	the surface	151:161	the surface of plant leaves	151:177	The production of superhydrophobic coatings inspired by the surface of plant leaves is a challenging goal.
33533623	12	15	theme	sol-gel	2256:2262	arg1	process					2264:2270	the sol-gel process	2252:2270	the sol-gel process	2252:2270	To mimic for the first time the abaxial surface, a templation approach was adopted using poly(dimethylsiloxane) (PDMS)/poly(methylphenylsiloxane) (PMPS) positive/negative replicas and a soft polymer/siloxane negative replica produced by the sol-gel process.
33533623	14	16	theme	harsh	2684:2688	arg1	climate					2704:2710	the harsh Mediterranean climate	2680:2710	the harsh Mediterranean climate	2680:2710	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	13	17	theme	texture	2431:2437	arg1	route					2540:2544	a suitable route	2529:2544	a suitable route to duplicate the abaxial surface with high fidelity	2529:2596	Because high topographical variations of the biotemplate and wax adhesion to the biohybrid film affected the replication quality, the reproduction of the wax texture via the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials is proposed as a suitable route to duplicate the abaxial surface with high fidelity.
33533623	13	17	theme	texture	2431:2437	arg1	reproduction					2407:2418	the reproduction	2403:2418	the reproduction of the wax texture via the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials	2403:2512	Because high topographical variations of the biotemplate and wax adhesion to the biohybrid film affected the replication quality, the reproduction of the wax texture via the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials is proposed as a suitable route to duplicate the abaxial surface with high fidelity.
33533623	11	18	dep	exchange	1940:1947	arg1	respiration					1969:1979	respiration	1969:1979	respiration	1969:1979	Such attributes enable efficient gas exchange (photosynthesis and respiration), transpiration, and evaporation.
33533623	11	18	dep	exchange	1940:1947	arg1	photosynthesis					1950:1963	photosynthesis	1950:1963	photosynthesis	1950:1963	Such attributes enable efficient gas exchange (photosynthesis and respiration), transpiration, and evaporation.
33533623	4	19	theme	hydrophobic	598:608	arg1	behaviors					631:639	feature hydrophobic and superhydrophobic behaviors	590:639	feature hydrophobic and superhydrophobic behaviors	590:639	The adaxial and abaxial surfaces feature hydrophobic and superhydrophobic behaviors, respectively.
33533623	10	20	theme	water	1743:1747	arg1	system					1760:1765	a sophisticated light and water management system	1717:1765	system	1760:1765	We show that the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet works as a sophisticated light and water management system: it reflects solar radiation diffusely to lower the surface temperature, and it has superhydrophobic character to keep the surface dry.
33533623	14	21	theme	chemical/physical	2611:2627	arg1	strategy					2629:2636	The natural chemical/physical strategy	2599:2636	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate	2599:2710	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	14	21	theme	chemical/physical	2611:2627	arg1	source					2726:2731	a powerful source	2715:2731	a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings	2715:2914	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	7	22	theme	texture	1281:1287	arg1	density					1220:1226	unusual variable density	1203:1226	unusual variable density	1203:1226	The measured tilting angles for the abaxial surface (12-70°) reveal unusual variable density and water adhesion of the nanostructured plate-based texture.
33533623	7	22	theme	texture	1281:1287	arg1	adhesion					1238:1245	water adhesion	1232:1245	water adhesion	1232:1245	The measured tilting angles for the abaxial surface (12-70°) reveal unusual variable density and water adhesion of the nanostructured plate-based texture.
33533623	4	23	theme	superhydrophobic	614:629	arg1	behaviors					631:639	feature hydrophobic and superhydrophobic behaviors	590:639	feature hydrophobic and superhydrophobic behaviors	590:639	The adaxial and abaxial surfaces feature hydrophobic and superhydrophobic behaviors, respectively.
33533623	14	24	theme	foreseen	2836:2843	arg1	applications					2845:2856	foreseen applications	2836:2856	foreseen applications as dual-functional antiglare and water-repelling coatings	2836:2914	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	10	25	theme	solar	1780:1784	arg1	radiation					1786:1794	solar radiation	1780:1794	solar radiation	1780:1794	We show that the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet works as a sophisticated light and water management system: it reflects solar radiation diffusely to lower the surface temperature, and it has superhydrophobic character to keep the surface dry.
33533623	12	26	theme	first	2032:2036	arg1	time					2038:2041	the first time	2028:2041	the first time	2028:2041	To mimic for the first time the abaxial surface, a templation approach was adopted using poly(dimethylsiloxane) (PDMS)/poly(methylphenylsiloxane) (PMPS) positive/negative replicas and a soft polymer/siloxane negative replica produced by the sol-gel process.
33533623	8	27	theme	nonglaucous	1436:1446	arg1	surface					1456:1462	the nonglaucous adaxial surface	1432:1462	the nonglaucous adaxial surface	1432:1462	Optical measurements demonstrate that light reflectance/absorbance of the glaucous abaxial surface is significantly higher/lower than that of the nonglaucous adaxial surface.
33533623	6	28	theme	abaxial	1118:1124	arg1	surface					1126:1132	the abaxial surface	1114:1132	the abaxial surface	1114:1132	In both surfaces, epicuticular wax is organized along a randomly oriented and intricate network of nanometer-thick and micrometer-long plates, whose density and degree of interconnection are significantly higher in the abaxial surface.
33533623	5	29	theme	chemical	662:669	arg1	composition					671:681	Their chemical composition	656:681	Their chemical composition	656:681	Their chemical composition is very simple: monopalmitin ester and palmitic acid are protuberant in the epicuticular and intracuticular wax layers of the adaxial surface, respectively, whereas 1-octacosanol dominates in the abaxial wax layers.
33533623	3	30	theme	Mediterranean	497:509	arg1	L					461:461	the Ceratonia silique L	439:461	the Ceratonia silique L. (carob)	439:470	This work is focused on the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world.
33533623	3	30	theme	Mediterranean	497:509	arg1	tree					511:514	a high-commercial-value Mediterranean tree	473:514	a high-commercial-value Mediterranean tree cultivated in many regions of the world	473:554	This work is focused on the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world.
33533623	2	31	from	markets	260:266	arg1	future					244:249	future	244:249	future	244:249	Such coatings hold a bright technological future in niche markets of the aeronautical, space, naval, building, automobile, and biomedical sectors.
33533623	14	32	theme	Mediterranean	2690:2702	arg1	climate					2704:2710	the harsh Mediterranean climate	2680:2710	the harsh Mediterranean climate	2680:2710	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	7	33	theme	measured	1139:1146	arg1	angles					1156:1161	The measured tilting angles	1135:1161	The measured tilting angles for the abaxial surface (12-70°)	1135:1194	The measured tilting angles for the abaxial surface (12-70°) reveal unusual variable density and water adhesion of the nanostructured plate-based texture.
33533623	1	34	theme	coatings	130:137	arg1	goal					196:199	a challenging goal	182:199	a challenging goal	182:199	The production of superhydrophobic coatings inspired by the surface of plant leaves is a challenging goal.
33533623	1	34	theme	coatings	130:137	arg1	production					99:108	The production	95:108	The production of superhydrophobic coatings inspired by the surface of plant leaves	95:177	The production of superhydrophobic coatings inspired by the surface of plant leaves is a challenging goal.
33533623	12	35	theme	polymer/siloxane	2206:2221	arg1	replica					2232:2238	a soft polymer/siloxane negative replica	2199:2238	a soft polymer/siloxane negative replica produced by the sol-gel process	2199:2270	To mimic for the first time the abaxial surface, a templation approach was adopted using poly(dimethylsiloxane) (PDMS)/poly(methylphenylsiloxane) (PMPS) positive/negative replicas and a soft polymer/siloxane negative replica produced by the sol-gel process.
33533623	10	36	theme	layer	1676:1680	arg1	texture					1640:1646	the highly dense nanostructured platelike texture	1598:1646	the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet	1598:1706	We show that the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet works as a sophisticated light and water management system: it reflects solar radiation diffusely to lower the surface temperature, and it has superhydrophobic character to keep the surface dry.
33533623	11	37	theme	efficient	1926:1934	arg1	exchange					1940:1947	efficient gas exchange	1926:1947	efficient gas exchange (photosynthesis and respiration)	1926:1980	Such attributes enable efficient gas exchange (photosynthesis and respiration), transpiration, and evaporation.
33533623	14	38	theme	systems	2823:2829	arg1	development					2759:2769	the development	2755:2769	the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings	2755:2914	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	5	39	from	layers	795:800	arg1	protuberant					740:750	protuberant	740:750	protuberant	740:750	Their chemical composition is very simple: monopalmitin ester and palmitic acid are protuberant in the epicuticular and intracuticular wax layers of the adaxial surface, respectively, whereas 1-octacosanol dominates in the abaxial wax layers.
33533623	3	40	dep	abaxial	395:401	arg1	bottom					404:409	bottom	404:409	bottom	404:409	This work is focused on the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world.
33533623	10	41	theme	platelike	1630:1638	arg1	texture					1640:1646	the highly dense nanostructured platelike texture	1598:1646	the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet	1598:1706	We show that the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet works as a sophisticated light and water management system: it reflects solar radiation diffusely to lower the surface temperature, and it has superhydrophobic character to keep the surface dry.
33533623	2	42	theme	biomedical	329:338	arg1	sectors					340:346	the aeronautical, space, naval, building, automobile, and biomedical sectors	271:346	the aeronautical, space, naval, building, automobile, and biomedical sectors	271:346	Such coatings hold a bright technological future in niche markets of the aeronautical, space, naval, building, automobile, and biomedical sectors.
33533623	13	43	theme	replication	2382:2392	arg1	quality					2394:2400	the replication quality	2378:2400	the replication quality	2378:2400	Because high topographical variations of the biotemplate and wax adhesion to the biohybrid film affected the replication quality, the reproduction of the wax texture via the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials is proposed as a suitable route to duplicate the abaxial surface with high fidelity.
33533623	6	44	theme	intricate	977:985	arg1	network					987:993	a randomly oriented and intricate network	953:993	a randomly oriented and intricate network	953:993	In both surfaces, epicuticular wax is organized along a randomly oriented and intricate network of nanometer-thick and micrometer-long plates, whose density and degree of interconnection are significantly higher in the abaxial surface.
33533623	13	45	theme	high	2584:2587	arg1	fidelity					2589:2596	high fidelity	2584:2596	high fidelity	2584:2596	Because high topographical variations of the biotemplate and wax adhesion to the biohybrid film affected the replication quality, the reproduction of the wax texture via the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials is proposed as a suitable route to duplicate the abaxial surface with high fidelity.
33533623	6	46	theme	oriented	964:971	arg1	network					987:993	a randomly oriented and intricate network	953:993	a randomly oriented and intricate network	953:993	In both surfaces, epicuticular wax is organized along a randomly oriented and intricate network of nanometer-thick and micrometer-long plates, whose density and degree of interconnection are significantly higher in the abaxial surface.
33533623	7	47	theme	nanostructured	1254:1267	arg1	texture					1281:1287	the nanostructured plate-based texture	1250:1287	the nanostructured plate-based texture	1250:1287	The measured tilting angles for the abaxial surface (12-70°) reveal unusual variable density and water adhesion of the nanostructured plate-based texture.
33533623	10	48	theme	leaflet	1700:1706	arg1	layer					1676:1680	the epicuticular abaxial layer	1651:1680	the epicuticular abaxial layer of the C. siliqua leaflet	1651:1706	We show that the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet works as a sophisticated light and water management system: it reflects solar radiation diffusely to lower the surface temperature, and it has superhydrophobic character to keep the surface dry.
33533623	13	49	theme	biohybrid	2354:2362	arg1	film					2364:2367	the biohybrid film	2350:2367	the biohybrid film	2350:2367	Because high topographical variations of the biotemplate and wax adhesion to the biohybrid film affected the replication quality, the reproduction of the wax texture via the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials is proposed as a suitable route to duplicate the abaxial surface with high fidelity.
33533623	10	50	theme	nanostructured	1615:1628	arg1	texture					1640:1646	the highly dense nanostructured platelike texture	1598:1646	the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet	1598:1706	We show that the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet works as a sophisticated light and water management system: it reflects solar radiation diffusely to lower the surface temperature, and it has superhydrophobic character to keep the surface dry.
33533623	3	51	theme	adaxial	377:383	arg1	surfaces					412:419	the adaxial (top) and abaxial (bottom) surfaces	373:419	the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world	373:554	This work is focused on the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world.
33533623	0	52	theme	Ceratonia	20:28	arg1	L					38:38	Ceratonia siliqua L	20:38	the Ceratonia siliqua L. (Carob) Leaflet	16:55	The Surfaces of the Ceratonia siliqua L. (Carob) Leaflet: Insights from Physics and Chemistry.
33533623	0	52	theme	Ceratonia	20:28	arg1	Carob					42:46	Carob	42:46	Carob	42:46	The Surfaces of the Ceratonia siliqua L. (Carob) Leaflet: Insights from Physics and Chemistry.
33533623	13	53	theme	biotemplate	2318:2328	arg1	variations					2300:2309	high topographical variations	2281:2309	high topographical variations of the biotemplate	2281:2328	Because high topographical variations of the biotemplate and wax adhesion to the biohybrid film affected the replication quality, the reproduction of the wax texture via the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials is proposed as a suitable route to duplicate the abaxial surface with high fidelity.
33533623	13	53	theme	biotemplate	2318:2328	arg1	adhesion					2338:2345	wax adhesion	2334:2345	wax adhesion to the biohybrid film	2334:2367	Because high topographical variations of the biotemplate and wax adhesion to the biohybrid film affected the replication quality, the reproduction of the wax texture via the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials is proposed as a suitable route to duplicate the abaxial surface with high fidelity.
33533623	7	54	theme	variable	1211:1218	arg1	density					1220:1226	unusual variable density	1203:1226	unusual variable density	1203:1226	The measured tilting angles for the abaxial surface (12-70°) reveal unusual variable density and water adhesion of the nanostructured plate-based texture.
33533623	3	55	theme	many	530:533	arg1	regions					535:541	many regions	530:541	many regions of the world	530:554	This work is focused on the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world.
33533623	10	56	theme	abaxial	1668:1674	arg1	layer					1676:1680	the epicuticular abaxial layer	1651:1680	the epicuticular abaxial layer of the C. siliqua leaflet	1651:1706	We show that the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet works as a sophisticated light and water management system: it reflects solar radiation diffusely to lower the surface temperature, and it has superhydrophobic character to keep the surface dry.
33533623	5	57	theme	monopalmitin	699:710	arg1	ester					712:716	monopalmitin ester	699:716	monopalmitin ester	699:716	Their chemical composition is very simple: monopalmitin ester and palmitic acid are protuberant in the epicuticular and intracuticular wax layers of the adaxial surface, respectively, whereas 1-octacosanol dominates in the abaxial wax layers.
33533623	9	58	theme	light	1562:1566	arg1	angle					1578:1582	the light incidence angle	1558:1582	the light incidence angle	1558:1582	In both surfaces, diffuse reflectance is dominant, and the absorbance is weakly dependent on the light incidence angle.
33533623	14	59	theme	C.	2653:2654	arg1	leaflet					2664:2670	the C. siliqua leaflet	2649:2670	the C. siliqua leaflet to face the harsh Mediterranean climate	2649:2710	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	2	60	theme	Such	202:205	arg1	coatings					207:214	Such coatings	202:214	Such coatings	202:214	Such coatings hold a bright technological future in niche markets of the aeronautical, space, naval, building, automobile, and biomedical sectors.
33533623	8	61	theme	abaxial	1373:1379	arg1	surface					1381:1387	the glaucous abaxial surface	1360:1387	the glaucous abaxial surface	1360:1387	Optical measurements demonstrate that light reflectance/absorbance of the glaucous abaxial surface is significantly higher/lower than that of the nonglaucous adaxial surface.
33533623	13	62	theme	topographical	2286:2298	arg1	variations					2300:2309	high topographical variations	2281:2309	high topographical variations of the biotemplate	2281:2328	Because high topographical variations of the biotemplate and wax adhesion to the biohybrid film affected the replication quality, the reproduction of the wax texture via the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials is proposed as a suitable route to duplicate the abaxial surface with high fidelity.
33533623	6	63	theme	plates	1034:1039	arg1	network					987:993	a randomly oriented and intricate network	953:993	a randomly oriented and intricate network	953:993	In both surfaces, epicuticular wax is organized along a randomly oriented and intricate network of nanometer-thick and micrometer-long plates, whose density and degree of interconnection are significantly higher in the abaxial surface.
33533623	10	64	theme	surface	1819:1825	arg1	temperature					1827:1837	the surface temperature	1815:1837	the surface temperature	1815:1837	We show that the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet works as a sophisticated light and water management system: it reflects solar radiation diffusely to lower the surface temperature, and it has superhydrophobic character to keep the surface dry.
33533623	12	65	theme	poly	2104:2107	arg1	replicas					2186:2193	poly(dimethylsiloxane) (PDMS)/poly(methylphenylsiloxane) (PMPS) positive/negative replicas	2104:2193	poly(dimethylsiloxane) (PDMS)/poly(methylphenylsiloxane) (PMPS) positive/negative replicas	2104:2193	To mimic for the first time the abaxial surface, a templation approach was adopted using poly(dimethylsiloxane) (PDMS)/poly(methylphenylsiloxane) (PMPS) positive/negative replicas and a soft polymer/siloxane negative replica produced by the sol-gel process.
33533623	12	65	theme	poly	2104:2107	arg1	PMPS					2162:2165	PMPS	2162:2165	PMPS	2162:2165	To mimic for the first time the abaxial surface, a templation approach was adopted using poly(dimethylsiloxane) (PDMS)/poly(methylphenylsiloxane) (PMPS) positive/negative replicas and a soft polymer/siloxane negative replica produced by the sol-gel process.
33533623	10	66	theme	C.	1689:1690	arg1	leaflet					1700:1706	the C. siliqua leaflet	1685:1706	the C. siliqua leaflet	1685:1706	We show that the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet works as a sophisticated light and water management system: it reflects solar radiation diffusely to lower the surface temperature, and it has superhydrophobic character to keep the surface dry.
33533623	10	67	theme	superhydrophobic	1851:1866	arg1	character					1868:1876	superhydrophobic character	1851:1876	superhydrophobic character to keep the surface dry	1851:1900	We show that the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet works as a sophisticated light and water management system: it reflects solar radiation diffusely to lower the surface temperature, and it has superhydrophobic character to keep the surface dry.
33533623	13	68	theme	1-octacosanol-grafted	2460:2480	arg1	materials					2504:2512	1-octacosanol-grafted siloxane-based hybrid materials	2460:2512	1-octacosanol-grafted siloxane-based hybrid materials	2460:2512	Because high topographical variations of the biotemplate and wax adhesion to the biohybrid film affected the replication quality, the reproduction of the wax texture via the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials is proposed as a suitable route to duplicate the abaxial surface with high fidelity.
33533623	14	69	theme	natural	2603:2609	arg1	strategy					2629:2636	The natural chemical/physical strategy	2599:2636	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate	2599:2710	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	14	69	theme	natural	2603:2609	arg1	source					2726:2731	a powerful source	2715:2731	a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings	2715:2914	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	13	70	theme	hybrid	2497:2502	arg1	materials					2504:2512	1-octacosanol-grafted siloxane-based hybrid materials	2460:2512	1-octacosanol-grafted siloxane-based hybrid materials	2460:2512	Because high topographical variations of the biotemplate and wax adhesion to the biohybrid film affected the replication quality, the reproduction of the wax texture via the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials is proposed as a suitable route to duplicate the abaxial surface with high fidelity.
33533623	9	71	from	surfaces	1473:1480	arg1	dominant					1506:1513	dominant	1506:1513	dominant	1506:1513	In both surfaces, diffuse reflectance is dominant, and the absorbance is weakly dependent on the light incidence angle.
33533623	9	72	theme	diffuse	1483:1489	arg1	reflectance					1491:1501	diffuse reflectance	1483:1501	diffuse reflectance	1483:1501	In both surfaces, diffuse reflectance is dominant, and the absorbance is weakly dependent on the light incidence angle.
33533623	3	73	theme	leaflet	428:434	arg1	surfaces					412:419	the adaxial (top) and abaxial (bottom) surfaces	373:419	the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world	373:554	This work is focused on the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world.
33533623	5	74	theme	epicuticular	759:770	arg1	layers					795:800	the epicuticular and intracuticular wax layers	755:800	the epicuticular and intracuticular wax layers of the adaxial surface	755:823	Their chemical composition is very simple: monopalmitin ester and palmitic acid are protuberant in the epicuticular and intracuticular wax layers of the adaxial surface, respectively, whereas 1-octacosanol dominates in the abaxial wax layers.
33533623	0	75	theme	L	38:38	arg1	Leaflet					49:55	the Ceratonia siliqua L. (Carob) Leaflet	16:55	the Ceratonia siliqua L. (Carob) Leaflet	16:55	The Surfaces of the Ceratonia siliqua L. (Carob) Leaflet: Insights from Physics and Chemistry.
33533623	14	76	theme	superhydrophobic	2797:2812	arg1	systems					2823:2829	diffuse reflecting and superhydrophobic material systems	2774:2829	diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings	2774:2914	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	5	77	theme	intracuticular	776:789	arg1	layers					795:800	the epicuticular and intracuticular wax layers	755:800	the epicuticular and intracuticular wax layers of the adaxial surface	755:823	Their chemical composition is very simple: monopalmitin ester and palmitic acid are protuberant in the epicuticular and intracuticular wax layers of the adaxial surface, respectively, whereas 1-octacosanol dominates in the abaxial wax layers.
33533623	14	78	theme	reflecting	2782:2791	arg1	systems					2823:2829	diffuse reflecting and superhydrophobic material systems	2774:2829	diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings	2774:2914	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	12	79	theme	templation	2066:2075	arg1	approach					2077:2084	a templation approach	2064:2084	a templation approach	2064:2084	To mimic for the first time the abaxial surface, a templation approach was adopted using poly(dimethylsiloxane) (PDMS)/poly(methylphenylsiloxane) (PMPS) positive/negative replicas and a soft polymer/siloxane negative replica produced by the sol-gel process.
33533623	14	80	theme	powerful	2717:2724	arg1	source					2726:2731	a powerful source	2715:2731	a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings	2715:2914	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	14	80	theme	powerful	2717:2724	arg1	strategy					2629:2636	The natural chemical/physical strategy	2599:2636	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate	2599:2710	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	13	81	theme	wax	2334:2336	arg1	adhesion					2338:2345	wax adhesion	2334:2345	wax adhesion to the biohybrid film	2334:2367	Because high topographical variations of the biotemplate and wax adhesion to the biohybrid film affected the replication quality, the reproduction of the wax texture via the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials is proposed as a suitable route to duplicate the abaxial surface with high fidelity.
33533623	3	82	theme	silique	453:459	arg1	L					461:461	the Ceratonia silique L	439:461	the Ceratonia silique L. (carob)	439:470	This work is focused on the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world.
33533623	3	82	theme	silique	453:459	arg1	carob					465:469	carob	465:469	carob	465:469	This work is focused on the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world.
33533623	3	82	theme	silique	453:459	arg1	tree					511:514	a high-commercial-value Mediterranean tree	473:514	a high-commercial-value Mediterranean tree cultivated in many regions of the world	473:554	This work is focused on the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world.
33533623	5	83	theme	adaxial	809:815	arg1	surface					817:823	the adaxial surface	805:823	the adaxial surface	805:823	Their chemical composition is very simple: monopalmitin ester and palmitic acid are protuberant in the epicuticular and intracuticular wax layers of the adaxial surface, respectively, whereas 1-octacosanol dominates in the abaxial wax layers.
33533623	10	84	theme	epicuticular	1655:1666	arg1	layer					1676:1680	the epicuticular abaxial layer	1651:1680	the epicuticular abaxial layer of the C. siliqua leaflet	1651:1706	We show that the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet works as a sophisticated light and water management system: it reflects solar radiation diffusely to lower the surface temperature, and it has superhydrophobic character to keep the surface dry.
33533623	13	85	theme	wax	2427:2429	arg1	texture					2431:2437	the wax texture	2423:2437	the wax texture	2423:2437	Because high topographical variations of the biotemplate and wax adhesion to the biohybrid film affected the replication quality, the reproduction of the wax texture via the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials is proposed as a suitable route to duplicate the abaxial surface with high fidelity.
33533623	11	86	theme	Such	1903:1906	arg1	attributes					1908:1917	Such attributes	1903:1917	Such attributes	1903:1917	Such attributes enable efficient gas exchange (photosynthesis and respiration), transpiration, and evaporation.
33533623	14	87	dep	face	2675:2678	arg1	climate					2704:2710	the harsh Mediterranean climate	2680:2710	the harsh Mediterranean climate	2680:2710	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	12	88	theme	abaxial	2047:2053	arg1	surface					2055:2061	the abaxial surface	2043:2061	the abaxial surface	2043:2061	To mimic for the first time the abaxial surface, a templation approach was adopted using poly(dimethylsiloxane) (PDMS)/poly(methylphenylsiloxane) (PMPS) positive/negative replicas and a soft polymer/siloxane negative replica produced by the sol-gel process.
33533623	14	89	theme	dual-functional	2861:2875	arg1	coatings					2907:2914	dual-functional antiglare and water-repelling coatings	2861:2914	dual-functional antiglare and water-repelling coatings	2861:2914	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	10	90	theme	management	1749:1758	arg1	system					1760:1765	a sophisticated light and water management system	1717:1765	system	1760:1765	We show that the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet works as a sophisticated light and water management system: it reflects solar radiation diffusely to lower the surface temperature, and it has superhydrophobic character to keep the surface dry.
33533623	5	91	theme	surface	817:823	arg1	layers					795:800	the epicuticular and intracuticular wax layers	755:800	the epicuticular and intracuticular wax layers of the adaxial surface	755:823	Their chemical composition is very simple: monopalmitin ester and palmitic acid are protuberant in the epicuticular and intracuticular wax layers of the adaxial surface, respectively, whereas 1-octacosanol dominates in the abaxial wax layers.
33533623	0	92	from	Physics	72:78	arg1	Insights					58:65	Insights	58:65	The Surfaces of the Ceratonia siliqua L. (Carob) Leaflet: Insights from Physics and Chemistry.	0:93	The Surfaces of the Ceratonia siliqua L. (Carob) Leaflet: Insights from Physics and Chemistry.
33533623	14	93	theme	bioinspiration	2736:2749	arg1	source					2726:2731	a powerful source	2715:2731	a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings	2715:2914	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	14	93	theme	bioinspiration	2736:2749	arg1	strategy					2629:2636	The natural chemical/physical strategy	2599:2636	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate	2599:2710	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	4	94	theme	feature	590:596	arg1	behaviors					631:639	feature hydrophobic and superhydrophobic behaviors	590:639	feature hydrophobic and superhydrophobic behaviors	590:639	The adaxial and abaxial surfaces feature hydrophobic and superhydrophobic behaviors, respectively.
33533623	3	95	dep	adaxial	377:383	arg1	top					386:388	top	386:388	top	386:388	This work is focused on the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world.
33533623	11	96	theme	gas	1936:1938	arg1	exchange					1940:1947	efficient gas exchange	1926:1947	efficient gas exchange (photosynthesis and respiration)	1926:1980	Such attributes enable efficient gas exchange (photosynthesis and respiration), transpiration, and evaporation.
33533623	5	97	from	protuberant	740:750	arg1	layers					795:800	the epicuticular and intracuticular wax layers	755:800	the epicuticular and intracuticular wax layers of the adaxial surface	755:823	Their chemical composition is very simple: monopalmitin ester and palmitic acid are protuberant in the epicuticular and intracuticular wax layers of the adaxial surface, respectively, whereas 1-octacosanol dominates in the abaxial wax layers.
33533623	10	98	theme	sophisticated	1719:1731	arg1	light					1733:1737	a sophisticated light and water management system	1717:1765	light	1733:1737	We show that the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet works as a sophisticated light and water management system: it reflects solar radiation diffusely to lower the surface temperature, and it has superhydrophobic character to keep the surface dry.
33533623	8	99	theme	adaxial	1448:1454	arg1	surface					1456:1462	the nonglaucous adaxial surface	1432:1462	the nonglaucous adaxial surface	1432:1462	Optical measurements demonstrate that light reflectance/absorbance of the glaucous abaxial surface is significantly higher/lower than that of the nonglaucous adaxial surface.
33533623	7	100	theme	tilting	1148:1154	arg1	angles					1156:1161	The measured tilting angles	1135:1161	The measured tilting angles for the abaxial surface (12-70°)	1135:1194	The measured tilting angles for the abaxial surface (12-70°) reveal unusual variable density and water adhesion of the nanostructured plate-based texture.
33533623	9	101	from	dominant	1506:1513	arg1	surfaces					1473:1480	both surfaces	1468:1480	both surfaces	1468:1480	In both surfaces, diffuse reflectance is dominant, and the absorbance is weakly dependent on the light incidence angle.
33533623	3	102	theme	high-commercial-value	475:495	arg1	L					461:461	the Ceratonia silique L	439:461	the Ceratonia silique L. (carob)	439:470	This work is focused on the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world.
33533623	3	102	theme	high-commercial-value	475:495	arg1	tree					511:514	a high-commercial-value Mediterranean tree	473:514	a high-commercial-value Mediterranean tree cultivated in many regions of the world	473:554	This work is focused on the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world.
33533623	2	103	from	future	244:249	arg1	markets					260:266	niche markets	254:266	niche markets of the aeronautical, space, naval, building, automobile, and biomedical sectors	254:346	Such coatings hold a bright technological future in niche markets of the aeronautical, space, naval, building, automobile, and biomedical sectors.
33533623	6	104	theme	epicuticular	917:928	arg1	wax					930:932	epicuticular wax	917:932	epicuticular wax	917:932	In both surfaces, epicuticular wax is organized along a randomly oriented and intricate network of nanometer-thick and micrometer-long plates, whose density and degree of interconnection are significantly higher in the abaxial surface.
33533623	12	105	theme	soft	2201:2204	arg1	replica					2232:2238	a soft polymer/siloxane negative replica	2199:2238	a soft polymer/siloxane negative replica produced by the sol-gel process	2199:2270	To mimic for the first time the abaxial surface, a templation approach was adopted using poly(dimethylsiloxane) (PDMS)/poly(methylphenylsiloxane) (PMPS) positive/negative replicas and a soft polymer/siloxane negative replica produced by the sol-gel process.
33533623	7	106	theme	plate-based	1269:1279	arg1	texture					1281:1287	the nanostructured plate-based texture	1250:1287	the nanostructured plate-based texture	1250:1287	The measured tilting angles for the abaxial surface (12-70°) reveal unusual variable density and water adhesion of the nanostructured plate-based texture.
33533623	4	107	theme	adaxial	561:567	arg1	surfaces					581:588	The adaxial and abaxial surfaces	557:588	The adaxial and abaxial surfaces	557:588	The adaxial and abaxial surfaces feature hydrophobic and superhydrophobic behaviors, respectively.
33533623	13	108	theme	abaxial	2563:2569	arg1	surface					2571:2577	the abaxial surface	2559:2577	the abaxial surface	2559:2577	Because high topographical variations of the biotemplate and wax adhesion to the biohybrid film affected the replication quality, the reproduction of the wax texture via the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials is proposed as a suitable route to duplicate the abaxial surface with high fidelity.
33533623	0	109	theme	Leaflet	49:55	arg1	Surfaces					4:11	The Surfaces	0:11	The Surfaces of the Ceratonia siliqua L. (Carob) Leaflet: Insights from Physics and Chemistry.	0:93	The Surfaces of the Ceratonia siliqua L. (Carob) Leaflet: Insights from Physics and Chemistry.
33533623	1	110	theme	superhydrophobic	113:128	arg1	coatings					130:137	superhydrophobic coatings	113:137	superhydrophobic coatings	113:137	The production of superhydrophobic coatings inspired by the surface of plant leaves is a challenging goal.
33533623	13	111	theme	high	2281:2284	arg1	variations					2300:2309	high topographical variations	2281:2309	high topographical variations of the biotemplate	2281:2328	Because high topographical variations of the biotemplate and wax adhesion to the biohybrid film affected the replication quality, the reproduction of the wax texture via the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials is proposed as a suitable route to duplicate the abaxial surface with high fidelity.
33533623	12	112	theme	negative	2223:2230	arg1	replica					2232:2238	a soft polymer/siloxane negative replica	2199:2238	a soft polymer/siloxane negative replica produced by the sol-gel process	2199:2270	To mimic for the first time the abaxial surface, a templation approach was adopted using poly(dimethylsiloxane) (PDMS)/poly(methylphenylsiloxane) (PMPS) positive/negative replicas and a soft polymer/siloxane negative replica produced by the sol-gel process.
33533623	4	113	theme	abaxial	573:579	arg1	surfaces					581:588	The adaxial and abaxial surfaces	557:588	The adaxial and abaxial surfaces	557:588	The adaxial and abaxial surfaces feature hydrophobic and superhydrophobic behaviors, respectively.
33533623	3	114	theme	L	461:461	arg1	leaflet					428:434	the leaflet	424:434	the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world	424:554	This work is focused on the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world.
33533623	12	115	theme	positive/negative	2168:2184	arg1	replicas					2186:2193	poly(dimethylsiloxane) (PDMS)/poly(methylphenylsiloxane) (PMPS) positive/negative replicas	2104:2193	poly(dimethylsiloxane) (PDMS)/poly(methylphenylsiloxane) (PMPS) positive/negative replicas	2104:2193	To mimic for the first time the abaxial surface, a templation approach was adopted using poly(dimethylsiloxane) (PDMS)/poly(methylphenylsiloxane) (PMPS) positive/negative replicas and a soft polymer/siloxane negative replica produced by the sol-gel process.
33533623	12	115	theme	positive/negative	2168:2184	arg1	PMPS					2162:2165	PMPS	2162:2165	PMPS	2162:2165	To mimic for the first time the abaxial surface, a templation approach was adopted using poly(dimethylsiloxane) (PDMS)/poly(methylphenylsiloxane) (PMPS) positive/negative replicas and a soft polymer/siloxane negative replica produced by the sol-gel process.
33533623	5	116	theme	abaxial	879:885	arg1	layers					891:896	the abaxial wax layers	875:896	the abaxial wax layers	875:896	Their chemical composition is very simple: monopalmitin ester and palmitic acid are protuberant in the epicuticular and intracuticular wax layers of the adaxial surface, respectively, whereas 1-octacosanol dominates in the abaxial wax layers.
33533623	2	117	theme	aeronautical	275:286	arg1	sectors					340:346	the aeronautical, space, naval, building, automobile, and biomedical sectors	271:346	the aeronautical, space, naval, building, automobile, and biomedical sectors	271:346	Such coatings hold a bright technological future in niche markets of the aeronautical, space, naval, building, automobile, and biomedical sectors.
33533623	5	118	theme	wax	887:889	arg1	layers					891:896	the abaxial wax layers	875:896	the abaxial wax layers	875:896	Their chemical composition is very simple: monopalmitin ester and palmitic acid are protuberant in the epicuticular and intracuticular wax layers of the adaxial surface, respectively, whereas 1-octacosanol dominates in the abaxial wax layers.
33533623	13	119	theme	suitable	2531:2538	arg1	route					2540:2544	a suitable route	2529:2544	a suitable route to duplicate the abaxial surface with high fidelity	2529:2596	Because high topographical variations of the biotemplate and wax adhesion to the biohybrid film affected the replication quality, the reproduction of the wax texture via the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials is proposed as a suitable route to duplicate the abaxial surface with high fidelity.
33533623	13	119	theme	suitable	2531:2538	arg1	reproduction					2407:2418	the reproduction	2403:2418	the reproduction of the wax texture via the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials	2403:2512	Because high topographical variations of the biotemplate and wax adhesion to the biohybrid film affected the replication quality, the reproduction of the wax texture via the synthesis of 1-octacosanol-grafted siloxane-based hybrid materials is proposed as a suitable route to duplicate the abaxial surface with high fidelity.
33533623	7	120	theme	water	1232:1236	arg1	adhesion					1238:1245	water adhesion	1232:1245	water adhesion	1232:1245	The measured tilting angles for the abaxial surface (12-70°) reveal unusual variable density and water adhesion of the nanostructured plate-based texture.
33533623	3	121	theme	world	550:554	arg1	regions					535:541	many regions	530:541	many regions of the world	530:554	This work is focused on the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world.
33533623	8	122	theme	Optical	1290:1296	arg1	measurements					1298:1309	Optical measurements	1290:1309	Optical measurements	1290:1309	Optical measurements demonstrate that light reflectance/absorbance of the glaucous abaxial surface is significantly higher/lower than that of the nonglaucous adaxial surface.
33533623	2	123	theme	automobile	313:322	arg1	sectors					340:346	the aeronautical, space, naval, building, automobile, and biomedical sectors	271:346	the aeronautical, space, naval, building, automobile, and biomedical sectors	271:346	Such coatings hold a bright technological future in niche markets of the aeronautical, space, naval, building, automobile, and biomedical sectors.
33533623	5	124	theme	palmitic	722:729	arg1	acid					731:734	palmitic acid	722:734	palmitic acid	722:734	Their chemical composition is very simple: monopalmitin ester and palmitic acid are protuberant in the epicuticular and intracuticular wax layers of the adaxial surface, respectively, whereas 1-octacosanol dominates in the abaxial wax layers.
33533623	10	125	theme	dense	1609:1613	arg1	texture					1640:1646	the highly dense nanostructured platelike texture	1598:1646	the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet	1598:1706	We show that the highly dense nanostructured platelike texture of the epicuticular abaxial layer of the C. siliqua leaflet works as a sophisticated light and water management system: it reflects solar radiation diffusely to lower the surface temperature, and it has superhydrophobic character to keep the surface dry.
33533623	6	126	theme	micrometer-long	1018:1032	arg1	plates					1034:1039	nanometer-thick and micrometer-long plates	998:1039	nanometer-thick and micrometer-long plates	998:1039	In both surfaces, epicuticular wax is organized along a randomly oriented and intricate network of nanometer-thick and micrometer-long plates, whose density and degree of interconnection are significantly higher in the abaxial surface.
33533623	0	127	theme	siliqua	30:36	arg1	L					38:38	Ceratonia siliqua L	20:38	the Ceratonia siliqua L. (Carob) Leaflet	16:55	The Surfaces of the Ceratonia siliqua L. (Carob) Leaflet: Insights from Physics and Chemistry.
33533623	0	127	theme	siliqua	30:36	arg1	Carob					42:46	Carob	42:46	Carob	42:46	The Surfaces of the Ceratonia siliqua L. (Carob) Leaflet: Insights from Physics and Chemistry.
33533623	7	128	theme	unusual	1203:1209	arg1	density					1220:1226	unusual variable density	1203:1226	unusual variable density	1203:1226	The measured tilting angles for the abaxial surface (12-70°) reveal unusual variable density and water adhesion of the nanostructured plate-based texture.
33533623	8	129	theme	light	1328:1332	arg1	reflectance/absorbance					1334:1355	light reflectance/absorbance	1328:1355	light reflectance/absorbance of the glaucous abaxial surface	1328:1387	Optical measurements demonstrate that light reflectance/absorbance of the glaucous abaxial surface is significantly higher/lower than that of the nonglaucous adaxial surface.
33533623	2	130	theme	sectors	340:346	arg1	markets					260:266	niche markets	254:266	niche markets of the aeronautical, space, naval, building, automobile, and biomedical sectors	254:346	Such coatings hold a bright technological future in niche markets of the aeronautical, space, naval, building, automobile, and biomedical sectors.
33533623	14	131	with	systems	2823:2829	arg1	applications					2845:2856	foreseen applications	2836:2856	foreseen applications as dual-functional antiglare and water-repelling coatings	2836:2914	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	14	132	theme	siliqua	2656:2662	arg1	leaflet					2664:2670	the C. siliqua leaflet	2649:2670	the C. siliqua leaflet to face the harsh Mediterranean climate	2649:2710	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	14	133	theme	antiglare	2877:2885	arg1	coatings					2907:2914	dual-functional antiglare and water-repelling coatings	2861:2914	dual-functional antiglare and water-repelling coatings	2861:2914	The natural chemical/physical strategy adopted by the C. siliqua leaflet to face the harsh Mediterranean climate is a powerful source of bioinspiration for the development of diffuse reflecting and superhydrophobic material systems with foreseen applications as dual-functional antiglare and water-repelling coatings.
33533623	2	134	theme	space	289:293	arg1	sectors					340:346	the aeronautical, space, naval, building, automobile, and biomedical sectors	271:346	the aeronautical, space, naval, building, automobile, and biomedical sectors	271:346	Such coatings hold a bright technological future in niche markets of the aeronautical, space, naval, building, automobile, and biomedical sectors.
33533623	1	135	dep	plant	166:170	arg1	leaves					172:177	leaves	172:177	leaves	172:177	The production of superhydrophobic coatings inspired by the surface of plant leaves is a challenging goal.
33533623	9	136	theme	incidence	1568:1576	arg1	angle					1578:1582	the light incidence angle	1558:1582	the light incidence angle	1558:1582	In both surfaces, diffuse reflectance is dominant, and the absorbance is weakly dependent on the light incidence angle.
33533623	8	137	theme	glaucous	1364:1371	arg1	surface					1381:1387	the glaucous abaxial surface	1360:1387	the glaucous abaxial surface	1360:1387	Optical measurements demonstrate that light reflectance/absorbance of the glaucous abaxial surface is significantly higher/lower than that of the nonglaucous adaxial surface.
33533623	3	138	theme	abaxial	395:401	arg1	surfaces					412:419	the adaxial (top) and abaxial (bottom) surfaces	373:419	the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world	373:554	This work is focused on the adaxial (top) and abaxial (bottom) surfaces of the leaflet of the Ceratonia silique L. (carob), a high-commercial-value Mediterranean tree cultivated in many regions of the world.
33533623	1	139	theme	challenging	184:194	arg1	goal					196:199	a challenging goal	182:199	a challenging goal	182:199	The production of superhydrophobic coatings inspired by the surface of plant leaves is a challenging goal.
33533623	1	139	theme	challenging	184:194	arg1	production					99:108	The production	95:108	The production of superhydrophobic coatings inspired by the surface of plant leaves	95:177	The production of superhydrophobic coatings inspired by the surface of plant leaves is a challenging goal.
33533623	0	140	dep	Surfaces	4:11	arg1	Insights					58:65	Insights	58:65	The Surfaces of the Ceratonia siliqua L. (Carob) Leaflet: Insights from Physics and Chemistry.	0:93	The Surfaces of the Ceratonia siliqua L. (Carob) Leaflet: Insights from Physics and Chemistry.
33533623	7	141	theme	abaxial	1171:1177	arg1	12-70°					1188:1193	12-70°	1188:1193	12-70°	1188:1193	The measured tilting angles for the abaxial surface (12-70°) reveal unusual variable density and water adhesion of the nanostructured plate-based texture.
33533623	7	141	theme	abaxial	1171:1177	arg1	surface					1179:1185	the abaxial surface	1167:1185	the abaxial surface (12-70°)	1167:1194	The measured tilting angles for the abaxial surface (12-70°) reveal unusual variable density and water adhesion of the nanostructured plate-based texture.
33533623	0	142	from	Chemistry	84:92	arg1	Insights					58:65	Insights	58:65	The Surfaces of the Ceratonia siliqua L. (Carob) Leaflet: Insights from Physics and Chemistry.	0:93	The Surfaces of the Ceratonia siliqua L. (Carob) Leaflet: Insights from Physics and Chemistry.
33533623	8	143	theme	surface	1381:1387	arg1	reflectance/absorbance					1334:1355	light reflectance/absorbance	1328:1355	light reflectance/absorbance of the glaucous abaxial surface	1328:1387	Optical measurements demonstrate that light reflectance/absorbance of the glaucous abaxial surface is significantly higher/lower than that of the nonglaucous adaxial surface.
33533623	2	144	theme	building	303:310	arg1	sectors					340:346	the aeronautical, space, naval, building, automobile, and biomedical sectors	271:346	the aeronautical, space, naval, building, automobile, and biomedical sectors	271:346	Such coatings hold a bright technological future in niche markets of the aeronautical, space, naval, building, automobile, and biomedical sectors.
33896287	2	0	from	content	414:420	arg1	chickens.2					433:442	broiler chickens.2	425:442	broiler chickens.2	425:442	The aim of this study was to examine the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower and finisher phase performance, total tract nutrient digestibility and excreta moisture content in broiler chickens.2.
33896287	7	1	theme	matter	1049:1054	arg1	content					1061:1067	Dry matter (DM) content	1045:1067	Dry matter (DM) content	1045:1067	Dry matter (DM) content was measured in the excreta and litter samples.
33896287	2	2	theme	phase	339:343	arg1	performance					345:355	phase performance	339:355	phase performance	339:355	The aim of this study was to examine the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower and finisher phase performance, total tract nutrient digestibility and excreta moisture content in broiler chickens.2.
33896287	0	3	from	digestibility	164:176	arg1	broilers					181:188	broilers	181:188	broilers	181:188	Dietary soluble non-starch polysaccharide level and composition influences grower and finisher phase performance, excreta moisture content and total tract nutrient digestibility in broilers.
33896287	12	4	theme	low	1850:1852	arg1	.6					1921:1922	the low compared to high sNSP level (P < 0.001 and P = 0.016, respectively).6	1846:1922	the low compared to high sNSP level (P < 0.001 and P = 0.016, respectively).6	1846:1922	In birds fed the barley- and sorghum-based diets, AMEn was lower when feeding the low compared to high sNSP level (P < 0.001 and P = 0.016, respectively).6.
33896287	3	5	theme	primary	532:538	arg1	sources					546:552	primary grain sources	532:552	primary grain sources (barley, corn, sorghum and wheat)	532:586	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	3	5	theme	primary	532:538	arg1	wheat					581:585	wheat	581:585	wheat	581:585	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	3	5	theme	primary	532:538	arg1	sorghum					569:575	sorghum	569:575	sorghum	569:575	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	3	5	theme	primary	532:538	arg1	corn					563:566	corn	563:566	corn	563:566	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	3	5	theme	primary	532:538	arg1	barley					555:560	barley	555:560	barley	555:560	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	6	6	theme	fresh	988:992	arg1	samples					1013:1019	fresh excreta and litter samples	988:1019	fresh excreta and litter samples	988:1019	For birds aged d 23 and 31, total pen body weight and feed intake were determined, and fresh excreta and litter samples were collected per pen.
33896287	2	7	theme	non-starch	261:270	arg1	level					294:298	dietary soluble non-starch polysaccharide (sNSP) level	245:298	dietary soluble non-starch polysaccharide (sNSP) level	245:298	The aim of this study was to examine the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower and finisher phase performance, total tract nutrient digestibility and excreta moisture content in broiler chickens.2.
33896287	10	8	dep	d	1573:1573	arg1	P = 0.045					1579:1587	P = 0.045	1579:1587	P = 0.045	1579:1587	In birds fed the corn-based diet, feeding low sNSP resulted in the lowest litter DM but highest DM digestibility at d 23 (P = 0.045 and P < 0.001) and d 31 (P = 0.022 and P = 0.008).
33896287	10	8	dep	d	1573:1573	arg1	P					1593:1593	P < 0.001	1593:1601	P < 0.001	1593:1601	In birds fed the corn-based diet, feeding low sNSP resulted in the lowest litter DM but highest DM digestibility at d 23 (P = 0.045 and P < 0.001) and d 31 (P = 0.022 and P = 0.008).
33896287	3	9	dep	treatments	494:503	arg1	diets					511:515	four diets	506:515	12 dietary treatments; four diets	483:515	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	4	10	contain	have	669:672	arg2	levels					701:706	similar protein and energy levels	674:706	levels	701:706	Diets were formulated to have similar protein and energy levels but differing sNSP levels, induced by manipulating the quantity of the ingredients in the diet.
33896287	4	10	contain	have	669:672	arg2	levels					727:732	sNSP levels	722:732	sNSP levels	722:732	Diets were formulated to have similar protein and energy levels but differing sNSP levels, induced by manipulating the quantity of the ingredients in the diet.
33896287	4	10	contain	have	669:672	arg1	Diets					644:648	Diets	644:648	Diets	644:648	Diets were formulated to have similar protein and energy levels but differing sNSP levels, induced by manipulating the quantity of the ingredients in the diet.
33896287	4	10	contain	have	669:672	arg2	protein					682:688	similar protein and energy levels	674:706	protein	682:688	Diets were formulated to have similar protein and energy levels but differing sNSP levels, induced by manipulating the quantity of the ingredients in the diet.
33896287	8	11	theme	insoluble	1223:1231	arg1	NSP					1233:1235	soluble and insoluble NSP and free oligosaccharide degradability	1211:1274	NSP	1233:1235	Total tract DM digestibility, apparent metabolisable energy corrected to nitrogen (AMEn), and soluble and insoluble NSP and free oligosaccharide degradability were evaluated.4.
33896287	0	12	theme	total	143:147	arg1	digestibility					164:176	total tract nutrient digestibility	143:176	total tract nutrient digestibility	143:176	Dietary soluble non-starch polysaccharide level and composition influences grower and finisher phase performance, excreta moisture content and total tract nutrient digestibility in broilers.
33896287	9	13	dep	sNSP	1411:1414	arg1	P = 0.004					1417:1425	P = 0.004	1417:1425	P = 0.004	1417:1425	In birds fed the sorghum- and corn-based diets, feeding high sNSP resulted in a lower cFCR at d 0-23 compared to low sNSP (P = 0.004 and P = 0.044, respectively).
33896287	9	13	dep	sNSP	1411:1414	arg1	P					1431:1431	P = 0.044	1431:1439	P = 0.044	1431:1439	In birds fed the sorghum- and corn-based diets, feeding high sNSP resulted in a lower cFCR at d 0-23 compared to low sNSP (P = 0.004 and P = 0.044, respectively).
33896287	4	14	from	quantity	763:770	arg1	diet					798:801	the diet	794:801	the diet	794:801	Diets were formulated to have similar protein and energy levels but differing sNSP levels, induced by manipulating the quantity of the ingredients in the diet.
33896287	0	15	from	grower	75:80	arg1	broilers					181:188	broilers	181:188	broilers	181:188	Dietary soluble non-starch polysaccharide level and composition influences grower and finisher phase performance, excreta moisture content and total tract nutrient digestibility in broilers.
33896287	8	16	theme	metabolisable	1156:1168	arg1	energy					1170:1175	apparent metabolisable energy	1147:1175	apparent metabolisable energy corrected to nitrogen (AMEn)	1147:1204	Total tract DM digestibility, apparent metabolisable energy corrected to nitrogen (AMEn), and soluble and insoluble NSP and free oligosaccharide degradability were evaluated.4.
33896287	0	17	theme	nutrient	155:162	arg1	digestibility					164:176	total tract nutrient digestibility	143:176	total tract nutrient digestibility	143:176	Dietary soluble non-starch polysaccharide level and composition influences grower and finisher phase performance, excreta moisture content and total tract nutrient digestibility in broilers.
33896287	10	18	theme	litter	1531:1536	arg1	DM					1538:1539	the lowest litter DM but highest DM digestibility	1520:1568	DM	1538:1539	In birds fed the corn-based diet, feeding low sNSP resulted in the lowest litter DM but highest DM digestibility at d 23 (P = 0.045 and P < 0.001) and d 31 (P = 0.022 and P = 0.008).
33896287	11	19	theme	high	1745:1748	arg1	sNSP					1750:1753	high sNSP	1745:1753	high sNSP (P < 0.001)	1745:1765	For all diets, degradability of sNSP was higher and insoluble NSP was lower when feeding low compared to high sNSP (P < 0.001).
33896287	11	19	theme	high	1745:1748	arg1	P < 0.001					1756:1764	P < 0.001	1756:1764	P < 0.001	1756:1764	For all diets, degradability of sNSP was higher and insoluble NSP was lower when feeding low compared to high sNSP (P < 0.001).
33896287	8	20	theme	oligosaccharide	1246:1260	arg1	degradability					1262:1274	soluble and insoluble NSP and free oligosaccharide degradability	1211:1274	degradability	1262:1274	Total tract DM digestibility, apparent metabolisable energy corrected to nitrogen (AMEn), and soluble and insoluble NSP and free oligosaccharide degradability were evaluated.4.
33896287	2	21	theme	dietary	245:251	arg1	level					294:298	dietary soluble non-starch polysaccharide (sNSP) level	245:298	dietary soluble non-starch polysaccharide (sNSP) level	245:298	The aim of this study was to examine the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower and finisher phase performance, total tract nutrient digestibility and excreta moisture content in broiler chickens.2.
33896287	9	22	from	d	1388:1388	arg1	cFCR					1380:1383	a lower cFCR	1372:1383	a lower cFCR at d 0-23	1372:1393	In birds fed the sorghum- and corn-based diets, feeding high sNSP resulted in a lower cFCR at d 0-23 compared to low sNSP (P = 0.004 and P = 0.044, respectively).
33896287	0	23	from	performance	101:111	arg1	broilers					181:188	broilers	181:188	broilers	181:188	Dietary soluble non-starch polysaccharide level and composition influences grower and finisher phase performance, excreta moisture content and total tract nutrient digestibility in broilers.
33896287	7	24	theme	Dry	1045:1047	arg1	DM					1057:1058	DM	1057:1058	DM	1057:1058	Dry matter (DM) content was measured in the excreta and litter samples.
33896287	7	24	theme	Dry	1045:1047	arg1	matter					1049:1054	Dry matter	1045:1054	Dry matter (DM) content	1045:1067	Dry matter (DM) content was measured in the excreta and litter samples.
33896287	10	25	theme	DM	1553:1554	arg1	digestibility					1556:1568	the lowest litter DM but highest DM digestibility	1520:1568	digestibility	1556:1568	In birds fed the corn-based diet, feeding low sNSP resulted in the lowest litter DM but highest DM digestibility at d 23 (P = 0.045 and P < 0.001) and d 31 (P = 0.022 and P = 0.008).
33896287	9	26	theme	sorghum-	1311:1318	arg1	diets					1335:1339	the sorghum- and corn-based diets	1307:1339	the sorghum- and corn-based diets	1307:1339	In birds fed the sorghum- and corn-based diets, feeding high sNSP resulted in a lower cFCR at d 0-23 compared to low sNSP (P = 0.004 and P = 0.044, respectively).
33896287	8	27	theme	tract	1123:1127	arg1	digestibility					1132:1144	Total tract DM digestibility	1117:1144	Total tract DM digestibility	1117:1144	Total tract DM digestibility, apparent metabolisable energy corrected to nitrogen (AMEn), and soluble and insoluble NSP and free oligosaccharide degradability were evaluated.4.
33896287	0	28	theme	finisher	86:93	arg1	performance					101:111	finisher phase performance	86:111	finisher phase performance	86:111	Dietary soluble non-starch polysaccharide level and composition influences grower and finisher phase performance, excreta moisture content and total tract nutrient digestibility in broilers.
33896287	4	29	from	diet	798:801	arg1	quantity					763:770	the quantity	759:770	the quantity of the ingredients in the diet	759:801	Diets were formulated to have similar protein and energy levels but differing sNSP levels, induced by manipulating the quantity of the ingredients in the diet.
33896287	9	30	theme	corn-based	1324:1333	arg1	diets					1335:1339	the sorghum- and corn-based diets	1307:1339	the sorghum- and corn-based diets	1307:1339	In birds fed the sorghum- and corn-based diets, feeding high sNSP resulted in a lower cFCR at d 0-23 compared to low sNSP (P = 0.004 and P = 0.044, respectively).
33896287	11	31	theme	sNSP	1672:1675	arg1	degradability					1655:1667	degradability	1655:1667	degradability of sNSP	1655:1675	For all diets, degradability of sNSP was higher and insoluble NSP was lower when feeding low compared to high sNSP (P < 0.001).
33896287	12	32	theme	sNSP	1871:1874	arg1	level					1876:1880	high sNSP level	1866:1880	high sNSP level (P < 0.001 and P = 0.016, respectively)	1866:1920	In birds fed the barley- and sorghum-based diets, AMEn was lower when feeding the low compared to high sNSP level (P < 0.001 and P = 0.016, respectively).6.
33896287	10	33	theme	low	1499:1501	arg1	sNSP					1503:1506	feeding low sNSP	1491:1506	feeding low sNSP	1491:1506	In birds fed the corn-based diet, feeding low sNSP resulted in the lowest litter DM but highest DM digestibility at d 23 (P = 0.045 and P < 0.001) and d 31 (P = 0.022 and P = 0.008).
33896287	8	34	theme	apparent	1147:1154	arg1	energy					1170:1175	apparent metabolisable energy	1147:1175	apparent metabolisable energy corrected to nitrogen (AMEn)	1147:1204	Total tract DM digestibility, apparent metabolisable energy corrected to nitrogen (AMEn), and soluble and insoluble NSP and free oligosaccharide degradability were evaluated.4.
33896287	0	35	theme	excreta	114:120	arg1	content					131:137	excreta moisture content	114:137	excreta moisture content	114:137	Dietary soluble non-starch polysaccharide level and composition influences grower and finisher phase performance, excreta moisture content and total tract nutrient digestibility in broilers.
33896287	9	36	theme	high	1350:1353	arg1	sNSP					1355:1358	feeding high sNSP	1342:1358	feeding high sNSP	1342:1358	In birds fed the sorghum- and corn-based diets, feeding high sNSP resulted in a lower cFCR at d 0-23 compared to low sNSP (P = 0.004 and P = 0.044, respectively).
33896287	4	37	theme	similar	674:680	arg1	protein					682:688	similar protein and energy levels	674:706	protein	682:688	Diets were formulated to have similar protein and energy levels but differing sNSP levels, induced by manipulating the quantity of the ingredients in the diet.
33896287	12	38	dep	level	1876:1880	arg1	P					1897:1897	P = 0.016	1897:1905	P = 0.016	1897:1905	In birds fed the barley- and sorghum-based diets, AMEn was lower when feeding the low compared to high sNSP level (P < 0.001 and P = 0.016, respectively).6.
33896287	12	38	dep	level	1876:1880	arg1	P < 0.001					1883:1891	P < 0.001	1883:1891	P < 0.001	1883:1891	In birds fed the barley- and sorghum-based diets, AMEn was lower when feeding the low compared to high sNSP level (P < 0.001 and P = 0.016, respectively).6.
33896287	2	39	theme	nutrient	370:377	arg1	digestibility					379:391	total tract nutrient digestibility	358:391	total tract nutrient digestibility	358:391	The aim of this study was to examine the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower and finisher phase performance, total tract nutrient digestibility and excreta moisture content in broiler chickens.2.
33896287	0	40	theme	Dietary	0:6	arg1	level					42:46	Dietary soluble non-starch polysaccharide level	0:46	Dietary soluble non-starch polysaccharide level	0:46	Dietary soluble non-starch polysaccharide level and composition influences grower and finisher phase performance, excreta moisture content and total tract nutrient digestibility in broilers.
33896287	11	41	theme	insoluble	1692:1700	arg1	NSP					1702:1704	insoluble NSP	1692:1704	insoluble NSP	1692:1704	For all diets, degradability of sNSP was higher and insoluble NSP was lower when feeding low compared to high sNSP (P < 0.001).
33896287	5	42	theme	d	849:849	arg1	0-12					851:854	d 0-12	849:854	d 0-12	849:854	The diets were fed in three phases, starter (d 0-12), grower (d 12-23) and finisher (d 23-31).3.
33896287	5	42	theme	d	849:849	arg1	starter					840:846	starter	840:846	starter (d 0-12)	840:855	The diets were fed in three phases, starter (d 0-12), grower (d 12-23) and finisher (d 23-31).3.
33896287	6	43	theme	aged	911:914	arg1	birds					905:909	birds	905:909	birds aged d 23 and 31	905:926	For birds aged d 23 and 31, total pen body weight and feed intake were determined, and fresh excreta and litter samples were collected per pen.
33896287	13	44	theme	broiler	1991:1997	arg1	performance					2010:2020	broiler productive performance	1991:2020	broiler productive performance	1991:2020	Results from this study showed that level of dietary sNSP impacts broiler productive performance and nutrient utilisation.
33896287	0	45	theme	non-starch	16:25	arg1	level					42:46	Dietary soluble non-starch polysaccharide level	0:46	Dietary soluble non-starch polysaccharide level	0:46	Dietary soluble non-starch polysaccharide level and composition influences grower and finisher phase performance, excreta moisture content and total tract nutrient digestibility in broilers.
33896287	10	46	theme	feeding	1491:1497	arg1	sNSP					1503:1506	feeding low sNSP	1491:1506	feeding low sNSP	1491:1506	In birds fed the corn-based diet, feeding low sNSP resulted in the lowest litter DM but highest DM digestibility at d 23 (P = 0.045 and P < 0.001) and d 31 (P = 0.022 and P = 0.008).
33896287	7	47	theme	litter	1101:1106	arg1	samples					1108:1114	the excreta and litter samples	1085:1114	the excreta and litter samples	1085:1114	Dry matter (DM) content was measured in the excreta and litter samples.
33896287	2	48	theme	moisture	405:412	arg1	content					414:420	excreta moisture content	397:420	excreta moisture content	397:420	The aim of this study was to examine the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower and finisher phase performance, total tract nutrient digestibility and excreta moisture content in broiler chickens.2.
33896287	13	49	theme	productive	1999:2008	arg1	performance					2010:2020	broiler productive performance	1991:2020	broiler productive performance	1991:2020	Results from this study showed that level of dietary sNSP impacts broiler productive performance and nutrient utilisation.
33896287	7	50	theme	excreta	1089:1095	arg1	samples					1108:1114	the excreta and litter samples	1085:1114	the excreta and litter samples	1085:1114	Dry matter (DM) content was measured in the excreta and litter samples.
33896287	10	51	dep	d	1608:1608	arg1	P					1628:1628	P = 0.008	1628:1636	P = 0.008	1628:1636	In birds fed the corn-based diet, feeding low sNSP resulted in the lowest litter DM but highest DM digestibility at d 23 (P = 0.045 and P < 0.001) and d 31 (P = 0.022 and P = 0.008).
33896287	10	51	dep	d	1608:1608	arg1	P = 0.022					1614:1622	P = 0.022	1614:1622	P = 0.022	1614:1622	In birds fed the corn-based diet, feeding low sNSP resulted in the lowest litter DM but highest DM digestibility at d 23 (P = 0.045 and P < 0.001) and d 31 (P = 0.022 and P = 0.008).
33896287	3	52	theme	different	598:606	arg1	levels					613:618	three different sNSP levels	592:618	three different sNSP levels (low, medium and high)	592:641	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	13	53	theme	dietary	1970:1976	arg1	sNSP					1978:1981	dietary sNSP	1970:1981	dietary sNSP	1970:1981	Results from this study showed that level of dietary sNSP impacts broiler productive performance and nutrient utilisation.
33896287	6	54	theme	body	939:942	arg1	weight					944:949	total pen body weight	929:949	total pen body weight	929:949	For birds aged d 23 and 31, total pen body weight and feed intake were determined, and fresh excreta and litter samples were collected per pen.
33896287	2	55	theme	total	358:362	arg1	digestibility					379:391	total tract nutrient digestibility	358:391	total tract nutrient digestibility	358:391	The aim of this study was to examine the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower and finisher phase performance, total tract nutrient digestibility and excreta moisture content in broiler chickens.2.
33896287	9	56	theme	low	1407:1409	arg1	sNSP					1411:1414	low sNSP	1407:1414	low sNSP (P = 0.004 and P = 0.044, respectively)	1407:1454	In birds fed the sorghum- and corn-based diets, feeding high sNSP resulted in a lower cFCR at d 0-23 compared to low sNSP (P = 0.004 and P = 0.044, respectively).
33896287	6	57	theme	total	929:933	arg1	weight					944:949	total pen body weight	929:949	total pen body weight	929:949	For birds aged d 23 and 31, total pen body weight and feed intake were determined, and fresh excreta and litter samples were collected per pen.
33896287	0	58	from	content	131:137	arg1	broilers					181:188	broilers	181:188	broilers	181:188	Dietary soluble non-starch polysaccharide level and composition influences grower and finisher phase performance, excreta moisture content and total tract nutrient digestibility in broilers.
33896287	3	59	theme	dietary	486:492	arg1	treatments					494:503	12 dietary treatments	483:503	12 dietary treatments; four diets	483:515	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	2	60	theme	sNSP	288:291	arg1	level					294:298	dietary soluble non-starch polysaccharide (sNSP) level	245:298	dietary soluble non-starch polysaccharide (sNSP) level	245:298	The aim of this study was to examine the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower and finisher phase performance, total tract nutrient digestibility and excreta moisture content in broiler chickens.2.
33896287	10	61	theme	lowest	1524:1529	arg1	DM					1538:1539	the lowest litter DM but highest DM digestibility	1520:1568	DM	1538:1539	In birds fed the corn-based diet, feeding low sNSP resulted in the lowest litter DM but highest DM digestibility at d 23 (P = 0.045 and P < 0.001) and d 31 (P = 0.022 and P = 0.008).
33896287	6	62	theme	excreta	994:1000	arg1	samples					1013:1019	fresh excreta and litter samples	988:1019	fresh excreta and litter samples	988:1019	For birds aged d 23 and 31, total pen body weight and feed intake were determined, and fresh excreta and litter samples were collected per pen.
33896287	2	63	theme	polysaccharide	272:285	arg1	level					294:298	dietary soluble non-starch polysaccharide (sNSP) level	245:298	dietary soluble non-starch polysaccharide (sNSP) level	245:298	The aim of this study was to examine the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower and finisher phase performance, total tract nutrient digestibility and excreta moisture content in broiler chickens.2.
33896287	2	64	from	performance	345:355	arg1	chickens.2					433:442	broiler chickens.2	425:442	broiler chickens.2	425:442	The aim of this study was to examine the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower and finisher phase performance, total tract nutrient digestibility and excreta moisture content in broiler chickens.2.
33896287	2	65	theme	composition	304:314	arg1	impact					235:240	the impact	231:240	the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower	231:324	The aim of this study was to examine the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower and finisher phase performance, total tract nutrient digestibility and excreta moisture content in broiler chickens.2.
33896287	4	66	theme	ingredients	779:789	arg1	quantity					763:770	the quantity	759:770	the quantity of the ingredients in the diet	759:801	Diets were formulated to have similar protein and energy levels but differing sNSP levels, induced by manipulating the quantity of the ingredients in the diet.
33896287	12	67	theme	barley-	1785:1791	arg1	diets					1811:1815	the barley- and sorghum-based diets	1781:1815	the barley- and sorghum-based diets	1781:1815	In birds fed the barley- and sorghum-based diets, AMEn was lower when feeding the low compared to high sNSP level (P < 0.001 and P = 0.016, respectively).6.
33896287	2	68	theme	level	294:298	arg1	impact					235:240	the impact	231:240	the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower	231:324	The aim of this study was to examine the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower and finisher phase performance, total tract nutrient digestibility and excreta moisture content in broiler chickens.2.
33896287	8	69	theme	Total	1117:1121	arg1	digestibility					1132:1144	Total tract DM digestibility	1117:1144	Total tract DM digestibility	1117:1144	Total tract DM digestibility, apparent metabolisable energy corrected to nitrogen (AMEn), and soluble and insoluble NSP and free oligosaccharide degradability were evaluated.4.
33896287	0	70	theme	tract	149:153	arg1	digestibility					164:176	total tract nutrient digestibility	143:176	total tract nutrient digestibility	143:176	Dietary soluble non-starch polysaccharide level and composition influences grower and finisher phase performance, excreta moisture content and total tract nutrient digestibility in broilers.
33896287	10	71	theme	highest	1545:1551	arg1	digestibility					1556:1568	the lowest litter DM but highest DM digestibility	1520:1568	digestibility	1556:1568	In birds fed the corn-based diet, feeding low sNSP resulted in the lowest litter DM but highest DM digestibility at d 23 (P = 0.045 and P < 0.001) and d 31 (P = 0.022 and P = 0.008).
33896287	8	72	theme	free	1241:1244	arg1	degradability					1262:1274	soluble and insoluble NSP and free oligosaccharide degradability	1211:1274	degradability	1262:1274	Total tract DM digestibility, apparent metabolisable energy corrected to nitrogen (AMEn), and soluble and insoluble NSP and free oligosaccharide degradability were evaluated.4.
33896287	2	73	theme	soluble	253:259	arg1	level					294:298	dietary soluble non-starch polysaccharide (sNSP) level	245:298	dietary soluble non-starch polysaccharide (sNSP) level	245:298	The aim of this study was to examine the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower and finisher phase performance, total tract nutrient digestibility and excreta moisture content in broiler chickens.2.
33896287	3	74	theme	grain	540:544	arg1	sources					546:552	primary grain sources	532:552	primary grain sources (barley, corn, sorghum and wheat)	532:586	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	3	74	theme	grain	540:544	arg1	wheat					581:585	wheat	581:585	wheat	581:585	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	3	74	theme	grain	540:544	arg1	sorghum					569:575	sorghum	569:575	sorghum	569:575	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	3	74	theme	grain	540:544	arg1	corn					563:566	corn	563:566	corn	563:566	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	3	74	theme	grain	540:544	arg1	barley					555:560	barley	555:560	barley	555:560	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	12	75	theme	sorghum-based	1797:1809	arg1	diets					1811:1815	the barley- and sorghum-based diets	1781:1815	the barley- and sorghum-based diets	1781:1815	In birds fed the barley- and sorghum-based diets, AMEn was lower when feeding the low compared to high sNSP level (P < 0.001 and P = 0.016, respectively).6.
33896287	6	76	theme	litter	1006:1011	arg1	samples					1013:1019	fresh excreta and litter samples	988:1019	fresh excreta and litter samples	988:1019	For birds aged d 23 and 31, total pen body weight and feed intake were determined, and fresh excreta and litter samples were collected per pen.
33896287	0	77	theme	phase	95:99	arg1	performance					101:111	finisher phase performance	86:111	finisher phase performance	86:111	Dietary soluble non-starch polysaccharide level and composition influences grower and finisher phase performance, excreta moisture content and total tract nutrient digestibility in broilers.
33896287	12	78	theme	high	1866:1869	arg1	level					1876:1880	high sNSP level	1866:1880	high sNSP level (P < 0.001 and P = 0.016, respectively)	1866:1920	In birds fed the barley- and sorghum-based diets, AMEn was lower when feeding the low compared to high sNSP level (P < 0.001 and P = 0.016, respectively).6.
33896287	9	79	dep	resulted	1360:1367	arg1	compared					1395:1402	compared	1395:1402	compared to low sNSP (P = 0.004 and P = 0.044, respectively)	1395:1454	In birds fed the sorghum- and corn-based diets, feeding high sNSP resulted in a lower cFCR at d 0-23 compared to low sNSP (P = 0.004 and P = 0.044, respectively).
33896287	3	80	dep	sources	546:552	arg1	sources					546:552	primary grain sources	532:552	primary grain sources (barley, corn, sorghum and wheat)	532:586	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	3	80	dep	sources	546:552	arg1	wheat					581:585	wheat	581:585	wheat	581:585	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	3	80	dep	sources	546:552	arg1	sorghum					569:575	sorghum	569:575	sorghum	569:575	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	3	80	dep	sources	546:552	arg1	corn					563:566	corn	563:566	corn	563:566	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	3	80	dep	sources	546:552	arg1	barley					555:560	barley	555:560	barley	555:560	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	8	81	theme	DM	1129:1130	arg1	digestibility					1132:1144	Total tract DM digestibility	1117:1144	Total tract DM digestibility	1117:1144	Total tract DM digestibility, apparent metabolisable energy corrected to nitrogen (AMEn), and soluble and insoluble NSP and free oligosaccharide degradability were evaluated.4.
33896287	4	82	theme	energy	694:699	arg1	levels					701:706	similar protein and energy levels	674:706	levels	701:706	Diets were formulated to have similar protein and energy levels but differing sNSP levels, induced by manipulating the quantity of the ingredients in the diet.
33896287	0	83	theme	moisture	122:129	arg1	content					131:137	excreta moisture content	114:137	excreta moisture content	114:137	Dietary soluble non-starch polysaccharide level and composition influences grower and finisher phase performance, excreta moisture content and total tract nutrient digestibility in broilers.
33896287	4	84	from	ingredients	779:789	arg1	diet					798:801	the diet	794:801	the diet	794:801	Diets were formulated to have similar protein and energy levels but differing sNSP levels, induced by manipulating the quantity of the ingredients in the diet.
33896287	9	85	theme	feeding	1342:1348	arg1	sNSP					1355:1358	feeding high sNSP	1342:1358	feeding high sNSP	1342:1358	In birds fed the sorghum- and corn-based diets, feeding high sNSP resulted in a lower cFCR at d 0-23 compared to low sNSP (P = 0.004 and P = 0.044, respectively).
33896287	3	86	theme	Cobb	445:448	arg1	broilers					454:461	Cobb 500 broilers	445:461	Cobb 500 broilers (n = 1080)	445:472	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	3	86	theme	Cobb	445:448	arg1	n = 1080					464:471	n = 1080	464:471	n = 1080	464:471	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	0	87	theme	soluble	8:14	arg1	level					42:46	Dietary soluble non-starch polysaccharide level	0:46	Dietary soluble non-starch polysaccharide level	0:46	Dietary soluble non-starch polysaccharide level and composition influences grower and finisher phase performance, excreta moisture content and total tract nutrient digestibility in broilers.
33896287	13	88	theme	nutrient	2026:2033	arg1	utilisation					2035:2045	nutrient utilisation	2026:2045	nutrient utilisation	2026:2045	Results from this study showed that level of dietary sNSP impacts broiler productive performance and nutrient utilisation.
33896287	6	89	dep	aged	911:914	arg1	d					916:916	d 23 and 31	916:926	d	916:916	For birds aged d 23 and 31, total pen body weight and feed intake were determined, and fresh excreta and litter samples were collected per pen.
33896287	6	89	dep	aged	911:914	arg1	31					925:926	31	925:926	31	925:926	For birds aged d 23 and 31, total pen body weight and feed intake were determined, and fresh excreta and litter samples were collected per pen.
33896287	2	90	theme	tract	364:368	arg1	digestibility					379:391	total tract nutrient digestibility	358:391	total tract nutrient digestibility	358:391	The aim of this study was to examine the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower and finisher phase performance, total tract nutrient digestibility and excreta moisture content in broiler chickens.2.
33896287	0	91	theme	polysaccharide	27:40	arg1	level					42:46	Dietary soluble non-starch polysaccharide level	0:46	Dietary soluble non-starch polysaccharide level	0:46	Dietary soluble non-starch polysaccharide level and composition influences grower and finisher phase performance, excreta moisture content and total tract nutrient digestibility in broilers.
33896287	2	92	from	impact	235:240	arg1	grower					319:324	grower	319:324	grower	319:324	The aim of this study was to examine the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower and finisher phase performance, total tract nutrient digestibility and excreta moisture content in broiler chickens.2.
33896287	2	93	theme	broiler	425:431	arg1	chickens.2					433:442	broiler chickens.2	425:442	broiler chickens.2	425:442	The aim of this study was to examine the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower and finisher phase performance, total tract nutrient digestibility and excreta moisture content in broiler chickens.2.
33896287	4	94	theme	differing	712:720	arg1	levels					727:732	sNSP levels	722:732	sNSP levels	722:732	Diets were formulated to have similar protein and energy levels but differing sNSP levels, induced by manipulating the quantity of the ingredients in the diet.
33896287	3	95	theme	sNSP	608:611	arg1	levels					613:618	three different sNSP levels	592:618	three different sNSP levels (low, medium and high)	592:641	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	9	96	theme	lower	1374:1378	arg1	cFCR					1380:1383	a lower cFCR	1372:1383	a lower cFCR at d 0-23	1372:1393	In birds fed the sorghum- and corn-based diets, feeding high sNSP resulted in a lower cFCR at d 0-23 compared to low sNSP (P = 0.004 and P = 0.044, respectively).
33896287	2	97	theme	excreta	397:403	arg1	content					414:420	excreta moisture content	397:420	excreta moisture content	397:420	The aim of this study was to examine the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower and finisher phase performance, total tract nutrient digestibility and excreta moisture content in broiler chickens.2.
33896287	6	98	theme	feed	955:958	arg1	intake					960:965	feed intake	955:965	feed intake	955:965	For birds aged d 23 and 31, total pen body weight and feed intake were determined, and fresh excreta and litter samples were collected per pen.
33896287	2	99	theme	study	210:214	arg1	aim					198:200	The aim	194:200	The aim of this study	194:214	The aim of this study was to examine the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower and finisher phase performance, total tract nutrient digestibility and excreta moisture content in broiler chickens.2.
33896287	5	100	theme	finisher	879:886	arg1	phases					832:837	three phases	826:837	three phases	826:837	The diets were fed in three phases, starter (d 0-12), grower (d 12-23) and finisher (d 23-31).3.
33896287	5	100	theme	finisher	879:886	arg1	.3					897:898	finisher (d 23-31).3	879:898	finisher (d 23-31).3	879:898	The diets were fed in three phases, starter (d 0-12), grower (d 12-23) and finisher (d 23-31).3.
33896287	2	101	from	digestibility	379:391	arg1	chickens.2					433:442	broiler chickens.2	425:442	broiler chickens.2	425:442	The aim of this study was to examine the impact of dietary soluble non-starch polysaccharide (sNSP) level and composition on grower and finisher phase performance, total tract nutrient digestibility and excreta moisture content in broiler chickens.2.
33896287	4	102	theme	sNSP	722:725	arg1	levels					727:732	sNSP levels	722:732	sNSP levels	722:732	Diets were formulated to have similar protein and energy levels but differing sNSP levels, induced by manipulating the quantity of the ingredients in the diet.
33896287	13	103	theme	sNSP	1978:1981	arg1	level					1961:1965	level	1961:1965	level of dietary sNSP	1961:1981	Results from this study showed that level of dietary sNSP impacts broiler productive performance and nutrient utilisation.
33896287	6	104	theme	pen	935:937	arg1	weight					944:949	total pen body weight	929:949	total pen body weight	929:949	For birds aged d 23 and 31, total pen body weight and feed intake were determined, and fresh excreta and litter samples were collected per pen.
33896287	8	105	theme	soluble	1211:1217	arg1	NSP					1233:1235	soluble and insoluble NSP and free oligosaccharide degradability	1211:1274	NSP	1233:1235	Total tract DM digestibility, apparent metabolisable energy corrected to nitrogen (AMEn), and soluble and insoluble NSP and free oligosaccharide degradability were evaluated.4.
33896287	13	106	from	study	1943:1947	arg1	Results					1925:1931	Results	1925:1931	Results from this study	1925:1947	Results from this study showed that level of dietary sNSP impacts broiler productive performance and nutrient utilisation.
33896287	10	107	theme	corn-based	1474:1483	arg1	diet					1485:1488	the corn-based diet	1470:1488	the corn-based diet	1470:1488	In birds fed the corn-based diet, feeding low sNSP resulted in the lowest litter DM but highest DM digestibility at d 23 (P = 0.045 and P < 0.001) and d 31 (P = 0.022 and P = 0.008).
33896287	3	108	dep	levels	613:618	arg1	low					621:623	low	621:623	low	621:623	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	3	108	dep	levels	613:618	arg1	high					637:640	high	637:640	high	637:640	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
33896287	3	108	dep	levels	613:618	arg1	medium					626:631	medium	626:631	medium	626:631	Cobb 500 broilers (n = 1080) were fed 12 dietary treatments; four diets with differing primary grain sources (barley, corn, sorghum and wheat) and three different sNSP levels (low, medium and high).
32035958	0	0	theme	dysbiosis	95:103	arg1	regulation					63:72	regulation	63:72	regulation of colonic microbial dysbiosis and inflammatory responses	63:130	Polysaccharide from flammuliana velutipes improves colitis via regulation of colonic microbial dysbiosis and inflammatory responses.
32035958	5	1	theme	signal	886:891	arg1	pathway					893:899	TLR4\NF-κB signal pathway	875:899	TLR4\NF-κB signal pathway	875:899	Our results showed that FVP treatment could regulate the colonic microbial dysbiosis and promote the levels of caecal short fatty chain acids, leading to down-regulation of TLR4\NF-κB signal pathway, which finally ameliorate the colitis.
32035958	5	2	theme	microbial	767:775	arg1	dysbiosis					777:785	the colonic microbial dysbiosis	755:785	the colonic microbial dysbiosis	755:785	Our results showed that FVP treatment could regulate the colonic microbial dysbiosis and promote the levels of caecal short fatty chain acids, leading to down-regulation of TLR4\NF-κB signal pathway, which finally ameliorate the colitis.
32035958	5	3	theme	chain	832:836	arg1	acids					838:842	caecal short fatty chain acids	813:842	caecal short fatty chain acids	813:842	Our results showed that FVP treatment could regulate the colonic microbial dysbiosis and promote the levels of caecal short fatty chain acids, leading to down-regulation of TLR4\NF-κB signal pathway, which finally ameliorate the colitis.
32035958	2	4	theme	-induced	337:344	arg1	colitis					346:352	dextran sulfate sodium (DSS)-induced colitis	309:352	dextran sulfate sodium (DSS)-induced colitis in rats	309:360	Effect of FVP on colitis was evaluated using dextran sulfate sodium (DSS)-induced colitis in rats.
32035958	4	5	theme	high	566:569	arg1	sequencing					582:591	rDNA high throughput sequencing	561:591	rDNA high throughput sequencing	561:591	The composition and taxonomy of colonic microbiota were analyzed by 16S rDNA high throughput sequencing, and the concentrations of caecal short fatty chain acids were assessed by chromatography-mass spectrometry.
32035958	0	6	theme	microbial	85:93	arg1	dysbiosis					95:103	colonic microbial dysbiosis	77:103	colonic microbial dysbiosis	77:103	Polysaccharide from flammuliana velutipes improves colitis via regulation of colonic microbial dysbiosis and inflammatory responses.
32035958	6	7	theme	FVP	1068:1070	arg1	application					1053:1063	the potential application	1039:1063	the potential application of FVP as a functional food ingredient or preventive drugs for colitis	1039:1134	Thus, the present study is the first attempt to elucidate the effect of FVP on colitis and support the potential application of FVP as a functional food ingredient or preventive drugs for colitis.
32035958	1	8	theme	colitis	255:261	arg1	prevention					241:250	the prevention	237:250	the prevention of colitis	237:261	The aim of this study is to investigate whether Flammuliana Velutipes Polysaccharide (FVP) could aid in the prevention of colitis.
32035958	0	9	theme	inflammatory	109:120	arg1	responses					122:130	inflammatory responses	109:130	inflammatory responses	109:130	Polysaccharide from flammuliana velutipes improves colitis via regulation of colonic microbial dysbiosis and inflammatory responses.
32035958	4	10	theme	acids	645:649	arg1	concentrations					602:615	the concentrations	598:615	the concentrations of caecal short fatty chain acids	598:649	The composition and taxonomy of colonic microbiota were analyzed by 16S rDNA high throughput sequencing, and the concentrations of caecal short fatty chain acids were assessed by chromatography-mass spectrometry.
32035958	6	11	theme	first	971:975	arg1	study					958:962	the present study	946:962	the present study	946:962	Thus, the present study is the first attempt to elucidate the effect of FVP on colitis and support the potential application of FVP as a functional food ingredient or preventive drugs for colitis.
32035958	6	11	theme	first	971:975	arg1	attempt					977:983	the first attempt	967:983	the first attempt to elucidate the effect of FVP on colitis and support the potential application of FVP as a functional food ingredient or preventive drugs for colitis	967:1134	Thus, the present study is the first attempt to elucidate the effect of FVP on colitis and support the potential application of FVP as a functional food ingredient or preventive drugs for colitis.
32035958	4	12	theme	throughput	571:580	arg1	sequencing					582:591	rDNA high throughput sequencing	561:591	rDNA high throughput sequencing	561:591	The composition and taxonomy of colonic microbiota were analyzed by 16S rDNA high throughput sequencing, and the concentrations of caecal short fatty chain acids were assessed by chromatography-mass spectrometry.
32035958	5	13	theme	acids	838:842	arg1	levels					803:808	the levels	799:808	the levels of caecal short fatty chain acids	799:842	Our results showed that FVP treatment could regulate the colonic microbial dysbiosis and promote the levels of caecal short fatty chain acids, leading to down-regulation of TLR4\NF-κB signal pathway, which finally ameliorate the colitis.
32035958	2	14	theme	FVP	274:276	arg1	Effect					264:269	Effect	264:269	Effect of FVP on colitis	264:287	Effect of FVP on colitis was evaluated using dextran sulfate sodium (DSS)-induced colitis in rats.
32035958	3	15	theme	related	414:420	arg1	biomarkers					422:431	related biomarkers	414:431	related biomarkers	414:431	Influence of FVP on the expression of inflammation related biomarkers and signal pathway element of TLR4\NF-κB were assessed.
32035958	0	16	theme	responses	122:130	arg1	regulation					63:72	regulation	63:72	regulation of colonic microbial dysbiosis and inflammatory responses	63:130	Polysaccharide from flammuliana velutipes improves colitis via regulation of colonic microbial dysbiosis and inflammatory responses.
32035958	3	17	from	Influence	363:371	arg1	expression					387:396	the expression	383:396	the expression of inflammation related biomarkers and signal pathway element of TLR4\NF-κB	383:472	Influence of FVP on the expression of inflammation related biomarkers and signal pathway element of TLR4\NF-κB were assessed.
32035958	6	18	from	effect	1002:1007	arg1	colitis					1019:1025	colitis	1019:1025	colitis	1019:1025	Thus, the present study is the first attempt to elucidate the effect of FVP on colitis and support the potential application of FVP as a functional food ingredient or preventive drugs for colitis.
32035958	1	19	theme	Flammuliana	181:191	arg1	Velutipes					193:201	Flammuliana Velutipes Polysaccharide (FVP)	181:222	Flammuliana Velutipes Polysaccharide (FVP)	181:222	The aim of this study is to investigate whether Flammuliana Velutipes Polysaccharide (FVP) could aid in the prevention of colitis.
32035958	6	20	theme	potential	1043:1051	arg1	application					1053:1063	the potential application	1039:1063	the potential application of FVP as a functional food ingredient or preventive drugs for colitis	1039:1134	Thus, the present study is the first attempt to elucidate the effect of FVP on colitis and support the potential application of FVP as a functional food ingredient or preventive drugs for colitis.
32035958	5	21	theme	caecal	813:818	arg1	acids					838:842	caecal short fatty chain acids	813:842	caecal short fatty chain acids	813:842	Our results showed that FVP treatment could regulate the colonic microbial dysbiosis and promote the levels of caecal short fatty chain acids, leading to down-regulation of TLR4\NF-κB signal pathway, which finally ameliorate the colitis.
32035958	5	22	theme	FVP	726:728	arg1	treatment					730:738	FVP treatment	726:738	FVP treatment	726:738	Our results showed that FVP treatment could regulate the colonic microbial dysbiosis and promote the levels of caecal short fatty chain acids, leading to down-regulation of TLR4\NF-κB signal pathway, which finally ameliorate the colitis.
32035958	3	23	theme	inflammation	401:412	arg1	expression					387:396	the expression	383:396	the expression of inflammation related biomarkers and signal pathway element of TLR4\NF-κB	383:472	Influence of FVP on the expression of inflammation related biomarkers and signal pathway element of TLR4\NF-κB were assessed.
32035958	5	24	theme	short	820:824	arg1	acids					838:842	caecal short fatty chain acids	813:842	caecal short fatty chain acids	813:842	Our results showed that FVP treatment could regulate the colonic microbial dysbiosis and promote the levels of caecal short fatty chain acids, leading to down-regulation of TLR4\NF-κB signal pathway, which finally ameliorate the colitis.
32035958	2	25	from	colitis	346:352	arg1	rats					357:360	rats	357:360	rats	357:360	Effect of FVP on colitis was evaluated using dextran sulfate sodium (DSS)-induced colitis in rats.
32035958	3	26	theme	pathway	444:450	arg1	element					452:458	signal pathway element	437:458	signal pathway element	437:458	Influence of FVP on the expression of inflammation related biomarkers and signal pathway element of TLR4\NF-κB were assessed.
32035958	0	27	theme	flammuliana	20:30	arg1	velutipes					32:40	flammuliana velutipes	20:40	flammuliana velutipes	20:40	Polysaccharide from flammuliana velutipes improves colitis via regulation of colonic microbial dysbiosis and inflammatory responses.
32035958	2	28	from	Effect	264:269	arg1	colitis					281:287	colitis	281:287	colitis	281:287	Effect of FVP on colitis was evaluated using dextran sulfate sodium (DSS)-induced colitis in rats.
32035958	3	29	theme	element	452:458	arg1	expression					387:396	the expression	383:396	the expression of inflammation related biomarkers and signal pathway element of TLR4\NF-κB	383:472	Influence of FVP on the expression of inflammation related biomarkers and signal pathway element of TLR4\NF-κB were assessed.
32035958	6	30	theme	present	950:956	arg1	study					958:962	the present study	946:962	the present study	946:962	Thus, the present study is the first attempt to elucidate the effect of FVP on colitis and support the potential application of FVP as a functional food ingredient or preventive drugs for colitis.
32035958	6	30	theme	present	950:956	arg1	attempt					977:983	the first attempt	967:983	the first attempt to elucidate the effect of FVP on colitis and support the potential application of FVP as a functional food ingredient or preventive drugs for colitis	967:1134	Thus, the present study is the first attempt to elucidate the effect of FVP on colitis and support the potential application of FVP as a functional food ingredient or preventive drugs for colitis.
32035958	1	31	dep	Velutipes	193:201	arg1	FVP					219:221	FVP	219:221	FVP	219:221	The aim of this study is to investigate whether Flammuliana Velutipes Polysaccharide (FVP) could aid in the prevention of colitis.
32035958	1	31	dep	Velutipes	193:201	arg1	Polysaccharide					203:216	Polysaccharide	203:216	Flammuliana Velutipes Polysaccharide (FVP)	181:222	The aim of this study is to investigate whether Flammuliana Velutipes Polysaccharide (FVP) could aid in the prevention of colitis.
32035958	5	32	theme	TLR4\NF-κB	875:884	arg1	pathway					893:899	TLR4\NF-κB signal pathway	875:899	TLR4\NF-κB signal pathway	875:899	Our results showed that FVP treatment could regulate the colonic microbial dysbiosis and promote the levels of caecal short fatty chain acids, leading to down-regulation of TLR4\NF-κB signal pathway, which finally ameliorate the colitis.
32035958	4	33	theme	fatty	633:637	arg1	acids					645:649	caecal short fatty chain acids	620:649	caecal short fatty chain acids	620:649	The composition and taxonomy of colonic microbiota were analyzed by 16S rDNA high throughput sequencing, and the concentrations of caecal short fatty chain acids were assessed by chromatography-mass spectrometry.
32035958	4	34	theme	chromatography-mass	668:686	arg1	spectrometry					688:699	chromatography-mass spectrometry	668:699	chromatography-mass spectrometry	668:699	The composition and taxonomy of colonic microbiota were analyzed by 16S rDNA high throughput sequencing, and the concentrations of caecal short fatty chain acids were assessed by chromatography-mass spectrometry.
32035958	4	35	theme	rDNA	561:564	arg1	sequencing					582:591	rDNA high throughput sequencing	561:591	rDNA high throughput sequencing	561:591	The composition and taxonomy of colonic microbiota were analyzed by 16S rDNA high throughput sequencing, and the concentrations of caecal short fatty chain acids were assessed by chromatography-mass spectrometry.
32035958	3	36	dep	inflammation	401:412	arg1	biomarkers					422:431	related biomarkers	414:431	related biomarkers	414:431	Influence of FVP on the expression of inflammation related biomarkers and signal pathway element of TLR4\NF-κB were assessed.
32035958	3	37	theme	signal	437:442	arg1	element					452:458	signal pathway element	437:458	signal pathway element	437:458	Influence of FVP on the expression of inflammation related biomarkers and signal pathway element of TLR4\NF-κB were assessed.
32035958	3	38	theme	FVP	376:378	arg1	Influence					363:371	Influence	363:371	Influence of FVP on the expression of inflammation related biomarkers and signal pathway element of TLR4\NF-κB	363:472	Influence of FVP on the expression of inflammation related biomarkers and signal pathway element of TLR4\NF-κB were assessed.
32035958	4	39	dep	composition	493:503	arg1	The					489:491	The	489:491	The	489:491	The composition and taxonomy of colonic microbiota were analyzed by 16S rDNA high throughput sequencing, and the concentrations of caecal short fatty chain acids were assessed by chromatography-mass spectrometry.
32035958	6	40	theme	functional	1077:1086	arg1	ingredient					1093:1102	a functional food ingredient	1075:1102	a functional food ingredient	1075:1102	Thus, the present study is the first attempt to elucidate the effect of FVP on colitis and support the potential application of FVP as a functional food ingredient or preventive drugs for colitis.
32035958	6	41	theme	preventive	1107:1116	arg1	drugs					1118:1122	preventive drugs	1107:1122	preventive drugs for colitis	1107:1134	Thus, the present study is the first attempt to elucidate the effect of FVP on colitis and support the potential application of FVP as a functional food ingredient or preventive drugs for colitis.
32035958	4	42	theme	microbiota	529:538	arg1	taxonomy					509:516	taxonomy	509:516	taxonomy	509:516	The composition and taxonomy of colonic microbiota were analyzed by 16S rDNA high throughput sequencing, and the concentrations of caecal short fatty chain acids were assessed by chromatography-mass spectrometry.
32035958	4	42	theme	microbiota	529:538	arg1	composition					493:503	composition	493:503	composition	493:503	The composition and taxonomy of colonic microbiota were analyzed by 16S rDNA high throughput sequencing, and the concentrations of caecal short fatty chain acids were assessed by chromatography-mass spectrometry.
32035958	4	43	theme	colonic	521:527	arg1	microbiota					529:538	colonic microbiota	521:538	colonic microbiota	521:538	The composition and taxonomy of colonic microbiota were analyzed by 16S rDNA high throughput sequencing, and the concentrations of caecal short fatty chain acids were assessed by chromatography-mass spectrometry.
32035958	6	44	theme	food	1088:1091	arg1	ingredient					1093:1102	a functional food ingredient	1075:1102	a functional food ingredient	1075:1102	Thus, the present study is the first attempt to elucidate the effect of FVP on colitis and support the potential application of FVP as a functional food ingredient or preventive drugs for colitis.
32035958	6	45	theme	FVP	1012:1014	arg1	effect					1002:1007	the effect	998:1007	the effect of FVP on colitis	998:1025	Thus, the present study is the first attempt to elucidate the effect of FVP on colitis and support the potential application of FVP as a functional food ingredient or preventive drugs for colitis.
32035958	3	46	theme	TLR4\NF-κB	463:472	arg1	inflammation					401:412	inflammation	401:412	inflammation	401:412	Influence of FVP on the expression of inflammation related biomarkers and signal pathway element of TLR4\NF-κB were assessed.
32035958	3	46	theme	TLR4\NF-κB	463:472	arg1	element					452:458	signal pathway element	437:458	signal pathway element	437:458	Influence of FVP on the expression of inflammation related biomarkers and signal pathway element of TLR4\NF-κB were assessed.
32035958	0	47	theme	colonic	77:83	arg1	dysbiosis					95:103	colonic microbial dysbiosis	77:103	colonic microbial dysbiosis	77:103	Polysaccharide from flammuliana velutipes improves colitis via regulation of colonic microbial dysbiosis and inflammatory responses.
32035958	4	48	theme	short	627:631	arg1	acids					645:649	caecal short fatty chain acids	620:649	caecal short fatty chain acids	620:649	The composition and taxonomy of colonic microbiota were analyzed by 16S rDNA high throughput sequencing, and the concentrations of caecal short fatty chain acids were assessed by chromatography-mass spectrometry.
32035958	4	49	theme	caecal	620:625	arg1	acids					645:649	caecal short fatty chain acids	620:649	caecal short fatty chain acids	620:649	The composition and taxonomy of colonic microbiota were analyzed by 16S rDNA high throughput sequencing, and the concentrations of caecal short fatty chain acids were assessed by chromatography-mass spectrometry.
32035958	1	50	theme	study	149:153	arg1	aim					137:139	The aim	133:139	The aim of this study	133:153	The aim of this study is to investigate whether Flammuliana Velutipes Polysaccharide (FVP) could aid in the prevention of colitis.
32035958	4	51	theme	chain	639:643	arg1	acids					645:649	caecal short fatty chain acids	620:649	caecal short fatty chain acids	620:649	The composition and taxonomy of colonic microbiota were analyzed by 16S rDNA high throughput sequencing, and the concentrations of caecal short fatty chain acids were assessed by chromatography-mass spectrometry.
32035958	5	52	theme	colonic	759:765	arg1	dysbiosis					777:785	the colonic microbial dysbiosis	755:785	the colonic microbial dysbiosis	755:785	Our results showed that FVP treatment could regulate the colonic microbial dysbiosis and promote the levels of caecal short fatty chain acids, leading to down-regulation of TLR4\NF-κB signal pathway, which finally ameliorate the colitis.
32035958	0	53	from	velutipes	32:40	arg1	Polysaccharide					0:13	Polysaccharide	0:13	Polysaccharide from flammuliana velutipes	0:40	Polysaccharide from flammuliana velutipes improves colitis via regulation of colonic microbial dysbiosis and inflammatory responses.
32035958	5	54	theme	fatty	826:830	arg1	acids					838:842	caecal short fatty chain acids	813:842	caecal short fatty chain acids	813:842	Our results showed that FVP treatment could regulate the colonic microbial dysbiosis and promote the levels of caecal short fatty chain acids, leading to down-regulation of TLR4\NF-κB signal pathway, which finally ameliorate the colitis.
32035958	5	55	theme	pathway	893:899	arg1	down-regulation					856:870	down-regulation	856:870	down-regulation of TLR4\NF-κB signal pathway, which finally ameliorate the colitis	856:937	Our results showed that FVP treatment could regulate the colonic microbial dysbiosis and promote the levels of caecal short fatty chain acids, leading to down-regulation of TLR4\NF-κB signal pathway, which finally ameliorate the colitis.
34576093	7	0	theme	precorneal	1169:1178	arg1	time					1190:1193	a longer precorneal retention time	1160:1193	a longer precorneal retention time	1160:1193	The area under the curve of DS in the aqueous humor in the DS-CDC-HP-Gel group was 3.45-fold that in the DS eye drops group, indicating a longer precorneal retention time.
34576093	7	1	from	group	1142:1146	arg1	area					1028:1031	The area	1024:1031	The area under the curve of DS in the aqueous humor in the DS-CDC-HP-Gel group	1024:1101	The area under the curve of DS in the aqueous humor in the DS-CDC-HP-Gel group was 3.45-fold that in the DS eye drops group, indicating a longer precorneal retention time.
34576093	7	1	from	group	1142:1146	arg1	3.45-fold					1107:1115	3.45-fold	1107:1115	3.45-fold	1107:1115	The area under the curve of DS in the aqueous humor in the DS-CDC-HP-Gel group was 3.45-fold that in the DS eye drops group, indicating a longer precorneal retention time.
34576093	7	2	theme	aqueous	1062:1068	arg1	humor					1070:1074	the aqueous humor	1058:1074	the aqueous humor in the DS-CDC-HP-Gel group	1058:1101	The area under the curve of DS in the aqueous humor in the DS-CDC-HP-Gel group was 3.45-fold that in the DS eye drops group, indicating a longer precorneal retention time.
34576093	1	3	theme	diclofenac	250:259	arg1	administration					232:245	the topical administration	220:245	the topical administration of diclofenac sodium (DS)	220:271	We developed a potential composite ocular drug delivery system for the topical administration of diclofenac sodium (DS).
34576093	4	4	theme	release	636:642	arg1	test					644:647	the in vitro release test	623:647	the in vitro release test	623:647	The results of the in vitro release test showed that this composite ocular drug delivery system (DS-CDC-HP-Gel) exhibited sustained release for 12 h.
34576093	2	5	theme	novel	278:282	arg1	CDC-HP					295:300	The novel carbon dot CDC-HP	274:300	The novel carbon dot CDC-HP	274:300	The novel carbon dot CDC-HP was synthesized by the pyrolysis of hyaluronic acid and carboxymethyl chitosan through a one-step hydrothermal method and then embedded in a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading.
34576093	6	6	theme	Elimination	935:945	arg1	profiles					947:954	Elimination profiles	935:954	Elimination profiles in tears	935:963	Elimination profiles in tears corresponded to the study of ex vivo fluorescence imaging.
34576093	1	7	theme	composite	178:186	arg1	system					209:214	a potential composite ocular drug delivery system	166:214	a potential composite ocular drug delivery system for the topical administration of diclofenac sodium (DS)	166:271	We developed a potential composite ocular drug delivery system for the topical administration of diclofenac sodium (DS).
34576093	4	8	theme	in	627:628	arg1	test					644:647	the in vitro release test	623:647	the in vitro release test	623:647	The results of the in vitro release test showed that this composite ocular drug delivery system (DS-CDC-HP-Gel) exhibited sustained release for 12 h.
34576093	3	9	theme	dots	584:587	arg1	characteristics					552:566	The physicochemical characteristics	532:566	The physicochemical characteristics of these carbon dots	532:587	The physicochemical characteristics of these carbon dots were investigated.
34576093	1	10	theme	drug	195:198	arg1	system					209:214	a potential composite ocular drug delivery system	166:214	a potential composite ocular drug delivery system for the topical administration of diclofenac sodium (DS)	166:271	We developed a potential composite ocular drug delivery system for the topical administration of diclofenac sodium (DS).
34576093	2	11	from	gel	467:469	arg1	embedded					429:436	embedded	429:436	embedded	429:436	The novel carbon dot CDC-HP was synthesized by the pyrolysis of hyaluronic acid and carboxymethyl chitosan through a one-step hydrothermal method and then embedded in a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading.
34576093	4	12	theme	delivery	688:695	arg1	DS-CDC-HP-Gel					705:717	DS-CDC-HP-Gel	705:717	DS-CDC-HP-Gel	705:717	The results of the in vitro release test showed that this composite ocular drug delivery system (DS-CDC-HP-Gel) exhibited sustained release for 12 h.
34576093	4	12	theme	delivery	688:695	arg1	system					697:702	this composite ocular drug delivery system	661:702	this composite ocular drug delivery system (DS-CDC-HP-Gel)	661:718	The results of the in vitro release test showed that this composite ocular drug delivery system (DS-CDC-HP-Gel) exhibited sustained release for 12 h.
34576093	5	13	from	distribution	796:807	arg1	tissues					819:825	ocular tissues	812:825	ocular tissues	812:825	The study of the ex vivo fluorescence distribution in ocular tissues showed that it could be used for bioimaging and tracing in ocular tissues and prolong precorneal retention.
34576093	1	14	dep	diclofenac	250:259	arg1	DS					269:270	DS	269:270	DS	269:270	We developed a potential composite ocular drug delivery system for the topical administration of diclofenac sodium (DS).
34576093	1	14	dep	diclofenac	250:259	arg1	sodium					261:266	sodium	261:266	diclofenac sodium (DS)	250:271	We developed a potential composite ocular drug delivery system for the topical administration of diclofenac sodium (DS).
34576093	5	15	theme	ocular	886:891	arg1	tissues					893:899	ocular tissues	886:899	ocular tissues	886:899	The study of the ex vivo fluorescence distribution in ocular tissues showed that it could be used for bioimaging and tracing in ocular tissues and prolong precorneal retention.
34576093	9	16	theme	potential	1453:1461	arg1	applications					1463:1474	potential applications	1453:1474	potential applications	1453:1474	This composite ocular delivery system possesses potential applications in ocular imaging and drug delivery.
34576093	8	17	theme	thermosensitive	1249:1263	arg1	gel					1273:1275	a thermosensitive in situ gel	1247:1275	a thermosensitive in situ gel	1247:1275	DS-CDC-HP with a positive charge and combined with a thermosensitive in situ gel might strengthen adherence to the corneal surface and prolong the ocular surface retention time to improve the bioavailability.
34576093	7	18	theme	longer	1162:1167	arg1	time					1190:1193	a longer precorneal retention time	1160:1193	a longer precorneal retention time	1160:1193	The area under the curve of DS in the aqueous humor in the DS-CDC-HP-Gel group was 3.45-fold that in the DS eye drops group, indicating a longer precorneal retention time.
34576093	5	19	from	study	762:766	arg1	tissues					819:825	ocular tissues	812:825	ocular tissues	812:825	The study of the ex vivo fluorescence distribution in ocular tissues showed that it could be used for bioimaging and tracing in ocular tissues and prolong precorneal retention.
34576093	5	20	theme	fluorescence	783:794	arg1	distribution					796:807	the ex vivo fluorescence distribution	771:807	the ex vivo fluorescence distribution in ocular tissues	771:825	The study of the ex vivo fluorescence distribution in ocular tissues showed that it could be used for bioimaging and tracing in ocular tissues and prolong precorneal retention.
34576093	9	21	theme	ocular	1479:1484	arg1	imaging					1486:1492	ocular imaging	1479:1492	ocular imaging	1479:1492	This composite ocular delivery system possesses potential applications in ocular imaging and drug delivery.
34576093	4	22	theme	ocular	676:681	arg1	DS-CDC-HP-Gel					705:717	DS-CDC-HP-Gel	705:717	DS-CDC-HP-Gel	705:717	The results of the in vitro release test showed that this composite ocular drug delivery system (DS-CDC-HP-Gel) exhibited sustained release for 12 h.
34576093	4	22	theme	ocular	676:681	arg1	system					697:702	this composite ocular drug delivery system	661:702	this composite ocular drug delivery system (DS-CDC-HP-Gel)	661:718	The results of the in vitro release test showed that this composite ocular drug delivery system (DS-CDC-HP-Gel) exhibited sustained release for 12 h.
34576093	0	23	theme	Ocular	65:70	arg1	System					86:91	a Composite Ocular Drug Delivery System	53:91	a Composite Ocular Drug Delivery System	53:91	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel for a Composite Ocular Drug Delivery System: Characterization, Ex-Vivo Imaging, and In Vivo Evaluation.
34576093	2	24	theme	poloxamer	492:500	arg1	188					502:504	poloxamer 407 and poloxamer 188	474:504	188	502:504	The novel carbon dot CDC-HP was synthesized by the pyrolysis of hyaluronic acid and carboxymethyl chitosan through a one-step hydrothermal method and then embedded in a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading.
34576093	0	25	theme	Delivery	77:84	arg1	System					86:91	a Composite Ocular Drug Delivery System	53:91	a Composite Ocular Drug Delivery System	53:91	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel for a Composite Ocular Drug Delivery System: Characterization, Ex-Vivo Imaging, and In Vivo Evaluation.
34576093	2	26	from	embedded	429:436	arg1	gel					467:469	a thermosensitive in situ gel	441:469	a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading	441:529	The novel carbon dot CDC-HP was synthesized by the pyrolysis of hyaluronic acid and carboxymethyl chitosan through a one-step hydrothermal method and then embedded in a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading.
34576093	2	27	theme	carboxymethyl	358:370	arg1	chitosan					372:379	carboxymethyl chitosan	358:379	carboxymethyl chitosan	358:379	The novel carbon dot CDC-HP was synthesized by the pyrolysis of hyaluronic acid and carboxymethyl chitosan through a one-step hydrothermal method and then embedded in a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading.
34576093	7	28	theme	eye	1132:1134	arg1	group					1142:1146	the DS eye drops group	1125:1146	the DS eye drops group	1125:1146	The area under the curve of DS in the aqueous humor in the DS-CDC-HP-Gel group was 3.45-fold that in the DS eye drops group, indicating a longer precorneal retention time.
34576093	2	29	theme	acid	349:352	arg1	pyrolysis					325:333	the pyrolysis	321:333	the pyrolysis of hyaluronic acid and carboxymethyl chitosan	321:379	The novel carbon dot CDC-HP was synthesized by the pyrolysis of hyaluronic acid and carboxymethyl chitosan through a one-step hydrothermal method and then embedded in a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading.
34576093	5	30	theme	ex	775:776	arg1	distribution					796:807	the ex vivo fluorescence distribution	771:807	the ex vivo fluorescence distribution in ocular tissues	771:825	The study of the ex vivo fluorescence distribution in ocular tissues showed that it could be used for bioimaging and tracing in ocular tissues and prolong precorneal retention.
34576093	4	31	theme	sustained	730:738	arg1	release					740:746	sustained release	730:746	sustained release for 12 h	730:755	The results of the in vitro release test showed that this composite ocular drug delivery system (DS-CDC-HP-Gel) exhibited sustained release for 12 h.
34576093	2	32	theme	one-step	391:398	arg1	method					413:418	a one-step hydrothermal method	389:418	a one-step hydrothermal method	389:418	The novel carbon dot CDC-HP was synthesized by the pyrolysis of hyaluronic acid and carboxymethyl chitosan through a one-step hydrothermal method and then embedded in a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading.
34576093	0	33	theme	Carbon	8:13	arg1	Gel					45:47	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel	0:47	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel for a Composite Ocular Drug Delivery System: Characterization, Ex-Vivo Imaging, and In Vivo Evaluation.	0:151	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel for a Composite Ocular Drug Delivery System: Characterization, Ex-Vivo Imaging, and In Vivo Evaluation.
34576093	5	34	theme	precorneal	913:922	arg1	retention					924:932	precorneal retention	913:932	precorneal retention	913:932	The study of the ex vivo fluorescence distribution in ocular tissues showed that it could be used for bioimaging and tracing in ocular tissues and prolong precorneal retention.
34576093	6	35	theme	imaging	1015:1021	arg1	study					985:989	the study	981:989	the study of ex vivo fluorescence imaging	981:1021	Elimination profiles in tears corresponded to the study of ex vivo fluorescence imaging.
34576093	2	36	theme	thermosensitive	443:457	arg1	gel					467:469	a thermosensitive in situ gel	441:469	a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading	441:529	The novel carbon dot CDC-HP was synthesized by the pyrolysis of hyaluronic acid and carboxymethyl chitosan through a one-step hydrothermal method and then embedded in a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading.
34576093	6	37	from	profiles	947:954	arg1	tears					959:963	tears	959:963	tears	959:963	Elimination profiles in tears corresponded to the study of ex vivo fluorescence imaging.
34576093	0	38	theme	In	37:38	arg1	Gel					45:47	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel	0:47	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel for a Composite Ocular Drug Delivery System: Characterization, Ex-Vivo Imaging, and In Vivo Evaluation.	0:151	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel for a Composite Ocular Drug Delivery System: Characterization, Ex-Vivo Imaging, and In Vivo Evaluation.
34576093	0	39	dep	Gel	45:47	arg1	Imaging					120:126	Ex-Vivo Imaging	112:126	Ex-Vivo Imaging	112:126	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel for a Composite Ocular Drug Delivery System: Characterization, Ex-Vivo Imaging, and In Vivo Evaluation.
34576093	0	39	dep	Gel	45:47	arg1	Evaluation					141:150	In Vivo Evaluation	133:150	In Vivo Evaluation	133:150	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel for a Composite Ocular Drug Delivery System: Characterization, Ex-Vivo Imaging, and In Vivo Evaluation.
34576093	0	39	dep	Gel	45:47	arg1	Characterization					94:109	Characterization	94:109	Characterization	94:109	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel for a Composite Ocular Drug Delivery System: Characterization, Ex-Vivo Imaging, and In Vivo Evaluation.
34576093	8	40	theme	ocular	1343:1348	arg1	time					1368:1371	the ocular surface retention time	1339:1371	the ocular surface retention time to improve the bioavailability	1339:1402	DS-CDC-HP with a positive charge and combined with a thermosensitive in situ gel might strengthen adherence to the corneal surface and prolong the ocular surface retention time to improve the bioavailability.
34576093	7	41	theme	DS-CDC-HP-Gel	1083:1095	arg1	group					1097:1101	the DS-CDC-HP-Gel group	1079:1101	the DS-CDC-HP-Gel group	1079:1101	The area under the curve of DS in the aqueous humor in the DS-CDC-HP-Gel group was 3.45-fold that in the DS eye drops group, indicating a longer precorneal retention time.
34576093	9	42	contain	possesses	1443:1451	arg1	system					1436:1441	This composite ocular delivery system	1405:1441	This composite ocular delivery system	1405:1441	This composite ocular delivery system possesses potential applications in ocular imaging and drug delivery.
34576093	9	42	contain	possesses	1443:1451	arg2	applications					1463:1474	potential applications	1453:1474	potential applications	1453:1474	This composite ocular delivery system possesses potential applications in ocular imaging and drug delivery.
34576093	2	43	dep	in	459:460	arg1	situ					462:465	situ	462:465	situ	462:465	The novel carbon dot CDC-HP was synthesized by the pyrolysis of hyaluronic acid and carboxymethyl chitosan through a one-step hydrothermal method and then embedded in a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading.
34576093	0	44	dep	In	37:38	arg1	Situ					40:43	Situ	40:43	Situ	40:43	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel for a Composite Ocular Drug Delivery System: Characterization, Ex-Vivo Imaging, and In Vivo Evaluation.
34576093	8	45	theme	retention	1358:1366	arg1	time					1368:1371	the ocular surface retention time	1339:1371	the ocular surface retention time to improve the bioavailability	1339:1402	DS-CDC-HP with a positive charge and combined with a thermosensitive in situ gel might strengthen adherence to the corneal surface and prolong the ocular surface retention time to improve the bioavailability.
34576093	8	46	dep	in	1265:1266	arg1	situ					1268:1271	situ	1268:1271	situ	1268:1271	DS-CDC-HP with a positive charge and combined with a thermosensitive in situ gel might strengthen adherence to the corneal surface and prolong the ocular surface retention time to improve the bioavailability.
34576093	1	47	theme	delivery	200:207	arg1	system					209:214	a potential composite ocular drug delivery system	166:214	a potential composite ocular drug delivery system for the topical administration of diclofenac sodium (DS)	166:271	We developed a potential composite ocular drug delivery system for the topical administration of diclofenac sodium (DS).
34576093	1	48	theme	topical	224:230	arg1	administration					232:245	the topical administration	220:245	the topical administration of diclofenac sodium (DS)	220:271	We developed a potential composite ocular drug delivery system for the topical administration of diclofenac sodium (DS).
34576093	7	49	theme	retention	1180:1188	arg1	time					1190:1193	a longer precorneal retention time	1160:1193	a longer precorneal retention time	1160:1193	The area under the curve of DS in the aqueous humor in the DS-CDC-HP-Gel group was 3.45-fold that in the DS eye drops group, indicating a longer precorneal retention time.
34576093	3	50	theme	physicochemical	536:550	arg1	characteristics					552:566	The physicochemical characteristics	532:566	The physicochemical characteristics of these carbon dots	532:587	The physicochemical characteristics of these carbon dots were investigated.
34576093	5	51	dep	ex	775:776	arg1	vivo					778:781	vivo	778:781	vivo	778:781	The study of the ex vivo fluorescence distribution in ocular tissues showed that it could be used for bioimaging and tracing in ocular tissues and prolong precorneal retention.
34576093	7	52	theme	DS	1052:1053	arg1	curve					1043:1047	the curve	1039:1047	the curve of DS in the aqueous humor in the DS-CDC-HP-Gel group	1039:1101	The area under the curve of DS in the aqueous humor in the DS-CDC-HP-Gel group was 3.45-fold that in the DS eye drops group, indicating a longer precorneal retention time.
34576093	8	53	with	DS-CDC-HP	1196:1204	arg1	charge					1222:1227	a positive charge	1211:1227	a positive charge	1211:1227	DS-CDC-HP with a positive charge and combined with a thermosensitive in situ gel might strengthen adherence to the corneal surface and prolong the ocular surface retention time to improve the bioavailability.
34576093	8	53	with	DS-CDC-HP	1196:1204	arg1	gel					1273:1275	a thermosensitive in situ gel	1247:1275	a thermosensitive in situ gel	1247:1275	DS-CDC-HP with a positive charge and combined with a thermosensitive in situ gel might strengthen adherence to the corneal surface and prolong the ocular surface retention time to improve the bioavailability.
34576093	2	54	theme	carbon	284:289	arg1	CDC-HP					295:300	The novel carbon dot CDC-HP	274:300	The novel carbon dot CDC-HP	274:300	The novel carbon dot CDC-HP was synthesized by the pyrolysis of hyaluronic acid and carboxymethyl chitosan through a one-step hydrothermal method and then embedded in a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading.
34576093	4	55	theme	test	644:647	arg1	results					612:618	The results	608:618	The results of the in vitro release test	608:647	The results of the in vitro release test showed that this composite ocular drug delivery system (DS-CDC-HP-Gel) exhibited sustained release for 12 h.
34576093	0	56	theme	Ex-Vivo	112:118	arg1	Imaging					120:126	Ex-Vivo Imaging	112:126	Ex-Vivo Imaging	112:126	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel for a Composite Ocular Drug Delivery System: Characterization, Ex-Vivo Imaging, and In Vivo Evaluation.
34576093	5	57	theme	distribution	796:807	arg1	study					762:766	The study	758:766	The study of the ex vivo fluorescence distribution in ocular tissues	758:825	The study of the ex vivo fluorescence distribution in ocular tissues showed that it could be used for bioimaging and tracing in ocular tissues and prolong precorneal retention.
34576093	2	58	theme	swelling	514:521	arg1	loading					523:529	swelling loading	514:529	swelling loading	514:529	The novel carbon dot CDC-HP was synthesized by the pyrolysis of hyaluronic acid and carboxymethyl chitosan through a one-step hydrothermal method and then embedded in a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading.
34576093	8	59	theme	in	1265:1266	arg1	gel					1273:1275	a thermosensitive in situ gel	1247:1275	a thermosensitive in situ gel	1247:1275	DS-CDC-HP with a positive charge and combined with a thermosensitive in situ gel might strengthen adherence to the corneal surface and prolong the ocular surface retention time to improve the bioavailability.
34576093	3	60	theme	carbon	577:582	arg1	dots					584:587	these carbon dots	571:587	these carbon dots	571:587	The physicochemical characteristics of these carbon dots were investigated.
34576093	5	61	theme	ocular	812:817	arg1	tissues					819:825	ocular tissues	812:825	ocular tissues	812:825	The study of the ex vivo fluorescence distribution in ocular tissues showed that it could be used for bioimaging and tracing in ocular tissues and prolong precorneal retention.
34576093	2	62	theme	188	502:504	arg1	gel					467:469	a thermosensitive in situ gel	441:469	a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading	441:529	The novel carbon dot CDC-HP was synthesized by the pyrolysis of hyaluronic acid and carboxymethyl chitosan through a one-step hydrothermal method and then embedded in a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading.
34576093	8	63	theme	positive	1213:1220	arg1	charge					1222:1227	a positive charge	1211:1227	a positive charge	1211:1227	DS-CDC-HP with a positive charge and combined with a thermosensitive in situ gel might strengthen adherence to the corneal surface and prolong the ocular surface retention time to improve the bioavailability.
34576093	0	64	theme	In	133:134	arg1	Evaluation					141:150	In Vivo Evaluation	133:150	In Vivo Evaluation	133:150	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel for a Composite Ocular Drug Delivery System: Characterization, Ex-Vivo Imaging, and In Vivo Evaluation.
34576093	5	65	used	used	851:854	arg2	it					839:840	it	839:840	it	839:840	The study of the ex vivo fluorescence distribution in ocular tissues showed that it could be used for bioimaging and tracing in ocular tissues and prolong precorneal retention.
34576093	5	66	from	tissues	819:825	arg1	study					762:766	The study	758:766	The study of the ex vivo fluorescence distribution in ocular tissues	758:825	The study of the ex vivo fluorescence distribution in ocular tissues showed that it could be used for bioimaging and tracing in ocular tissues and prolong precorneal retention.
34576093	4	67	theme	drug	683:686	arg1	DS-CDC-HP-Gel					705:717	DS-CDC-HP-Gel	705:717	DS-CDC-HP-Gel	705:717	The results of the in vitro release test showed that this composite ocular drug delivery system (DS-CDC-HP-Gel) exhibited sustained release for 12 h.
34576093	4	67	theme	drug	683:686	arg1	system					697:702	this composite ocular drug delivery system	661:702	this composite ocular drug delivery system (DS-CDC-HP-Gel)	661:718	The results of the in vitro release test showed that this composite ocular drug delivery system (DS-CDC-HP-Gel) exhibited sustained release for 12 h.
34576093	0	68	dep	In	133:134	arg1	Vivo					136:139	Vivo	136:139	Vivo	136:139	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel for a Composite Ocular Drug Delivery System: Characterization, Ex-Vivo Imaging, and In Vivo Evaluation.
34576093	6	69	theme	ex	994:995	arg1	imaging					1015:1021	ex vivo fluorescence imaging	994:1021	ex vivo fluorescence imaging	994:1021	Elimination profiles in tears corresponded to the study of ex vivo fluorescence imaging.
34576093	0	70	theme	Composite	55:63	arg1	System					86:91	a Composite Ocular Drug Delivery System	53:91	a Composite Ocular Drug Delivery System	53:91	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel for a Composite Ocular Drug Delivery System: Characterization, Ex-Vivo Imaging, and In Vivo Evaluation.
34576093	1	71	theme	potential	168:176	arg1	system					209:214	a potential composite ocular drug delivery system	166:214	a potential composite ocular drug delivery system for the topical administration of diclofenac sodium (DS)	166:271	We developed a potential composite ocular drug delivery system for the topical administration of diclofenac sodium (DS).
34576093	0	72	theme	Drug	72:75	arg1	System					86:91	a Composite Ocular Drug Delivery System	53:91	a Composite Ocular Drug Delivery System	53:91	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel for a Composite Ocular Drug Delivery System: Characterization, Ex-Vivo Imaging, and In Vivo Evaluation.
34576093	2	73	theme	in	459:460	arg1	gel					467:469	a thermosensitive in situ gel	441:469	a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading	441:529	The novel carbon dot CDC-HP was synthesized by the pyrolysis of hyaluronic acid and carboxymethyl chitosan through a one-step hydrothermal method and then embedded in a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading.
34576093	4	74	theme	composite	666:674	arg1	DS-CDC-HP-Gel					705:717	DS-CDC-HP-Gel	705:717	DS-CDC-HP-Gel	705:717	The results of the in vitro release test showed that this composite ocular drug delivery system (DS-CDC-HP-Gel) exhibited sustained release for 12 h.
34576093	4	74	theme	composite	666:674	arg1	system					697:702	this composite ocular drug delivery system	661:702	this composite ocular drug delivery system (DS-CDC-HP-Gel)	661:718	The results of the in vitro release test showed that this composite ocular drug delivery system (DS-CDC-HP-Gel) exhibited sustained release for 12 h.
34576093	7	75	theme	drops	1136:1140	arg1	group					1142:1146	the DS eye drops group	1125:1146	the DS eye drops group	1125:1146	The area under the curve of DS in the aqueous humor in the DS-CDC-HP-Gel group was 3.45-fold that in the DS eye drops group, indicating a longer precorneal retention time.
34576093	9	76	theme	drug	1498:1501	arg1	delivery					1503:1510	drug delivery	1498:1510	drug delivery	1498:1510	This composite ocular delivery system possesses potential applications in ocular imaging and drug delivery.
34576093	7	77	from	3.45-fold	1107:1115	arg1	group					1142:1146	the DS eye drops group	1125:1146	the DS eye drops group	1125:1146	The area under the curve of DS in the aqueous humor in the DS-CDC-HP-Gel group was 3.45-fold that in the DS eye drops group, indicating a longer precorneal retention time.
34576093	7	78	theme	DS	1129:1130	arg1	group					1142:1146	the DS eye drops group	1125:1146	the DS eye drops group	1125:1146	The area under the curve of DS in the aqueous humor in the DS-CDC-HP-Gel group was 3.45-fold that in the DS eye drops group, indicating a longer precorneal retention time.
34576093	9	79	theme	composite	1410:1418	arg1	system					1436:1441	This composite ocular delivery system	1405:1441	This composite ocular delivery system	1405:1441	This composite ocular delivery system possesses potential applications in ocular imaging and drug delivery.
34576093	2	80	theme	poloxamer	474:482	arg1	gel					467:469	a thermosensitive in situ gel	441:469	a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading	441:529	The novel carbon dot CDC-HP was synthesized by the pyrolysis of hyaluronic acid and carboxymethyl chitosan through a one-step hydrothermal method and then embedded in a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading.
34576093	6	81	dep	ex	994:995	arg1	vivo					997:1000	vivo	997:1000	vivo	997:1000	Elimination profiles in tears corresponded to the study of ex vivo fluorescence imaging.
34576093	0	82	theme	Novel	2:6	arg1	Gel					45:47	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel	0:47	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel for a Composite Ocular Drug Delivery System: Characterization, Ex-Vivo Imaging, and In Vivo Evaluation.	0:151	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel for a Composite Ocular Drug Delivery System: Characterization, Ex-Vivo Imaging, and In Vivo Evaluation.
34576093	7	83	from	curve	1043:1047	arg1	humor					1070:1074	the aqueous humor	1058:1074	the aqueous humor in the DS-CDC-HP-Gel group	1058:1101	The area under the curve of DS in the aqueous humor in the DS-CDC-HP-Gel group was 3.45-fold that in the DS eye drops group, indicating a longer precorneal retention time.
34576093	2	84	theme	hyaluronic	338:347	arg1	acid					349:352	hyaluronic acid	338:352	hyaluronic acid	338:352	The novel carbon dot CDC-HP was synthesized by the pyrolysis of hyaluronic acid and carboxymethyl chitosan through a one-step hydrothermal method and then embedded in a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading.
34576093	0	85	theme	Dots/Thermo-Sensitive	15:35	arg1	Gel					45:47	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel	0:47	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel for a Composite Ocular Drug Delivery System: Characterization, Ex-Vivo Imaging, and In Vivo Evaluation.	0:151	A Novel Carbon Dots/Thermo-Sensitive In Situ Gel for a Composite Ocular Drug Delivery System: Characterization, Ex-Vivo Imaging, and In Vivo Evaluation.
34576093	2	86	theme	hydrothermal	400:411	arg1	method					413:418	a one-step hydrothermal method	389:418	a one-step hydrothermal method	389:418	The novel carbon dot CDC-HP was synthesized by the pyrolysis of hyaluronic acid and carboxymethyl chitosan through a one-step hydrothermal method and then embedded in a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading.
34576093	7	87	from	DS	1052:1053	arg1	humor					1070:1074	the aqueous humor	1058:1074	the aqueous humor in the DS-CDC-HP-Gel group	1058:1101	The area under the curve of DS in the aqueous humor in the DS-CDC-HP-Gel group was 3.45-fold that in the DS eye drops group, indicating a longer precorneal retention time.
34576093	8	88	theme	corneal	1311:1317	arg1	surface					1319:1325	the corneal surface	1307:1325	the corneal surface	1307:1325	DS-CDC-HP with a positive charge and combined with a thermosensitive in situ gel might strengthen adherence to the corneal surface and prolong the ocular surface retention time to improve the bioavailability.
34576093	1	89	theme	ocular	188:193	arg1	system					209:214	a potential composite ocular drug delivery system	166:214	a potential composite ocular drug delivery system for the topical administration of diclofenac sodium (DS)	166:271	We developed a potential composite ocular drug delivery system for the topical administration of diclofenac sodium (DS).
34576093	4	90	dep	in	627:628	arg1	vitro					630:634	vitro	630:634	vitro	630:634	The results of the in vitro release test showed that this composite ocular drug delivery system (DS-CDC-HP-Gel) exhibited sustained release for 12 h.
34576093	6	91	theme	fluorescence	1002:1013	arg1	imaging					1015:1021	ex vivo fluorescence imaging	994:1021	ex vivo fluorescence imaging	994:1021	Elimination profiles in tears corresponded to the study of ex vivo fluorescence imaging.
34576093	2	92	theme	chitosan	372:379	arg1	pyrolysis					325:333	the pyrolysis	321:333	the pyrolysis of hyaluronic acid and carboxymethyl chitosan	321:379	The novel carbon dot CDC-HP was synthesized by the pyrolysis of hyaluronic acid and carboxymethyl chitosan through a one-step hydrothermal method and then embedded in a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading.
34576093	9	93	theme	ocular	1420:1425	arg1	system					1436:1441	This composite ocular delivery system	1405:1441	This composite ocular delivery system	1405:1441	This composite ocular delivery system possesses potential applications in ocular imaging and drug delivery.
34576093	7	94	from	humor	1070:1074	arg1	group					1097:1101	the DS-CDC-HP-Gel group	1079:1101	the DS-CDC-HP-Gel group	1079:1101	The area under the curve of DS in the aqueous humor in the DS-CDC-HP-Gel group was 3.45-fold that in the DS eye drops group, indicating a longer precorneal retention time.
34576093	7	94	from	humor	1070:1074	arg1	curve					1043:1047	the curve	1039:1047	the curve of DS in the aqueous humor in the DS-CDC-HP-Gel group	1039:1101	The area under the curve of DS in the aqueous humor in the DS-CDC-HP-Gel group was 3.45-fold that in the DS eye drops group, indicating a longer precorneal retention time.
34576093	8	95	theme	surface	1350:1356	arg1	time					1368:1371	the ocular surface retention time	1339:1371	the ocular surface retention time to improve the bioavailability	1339:1402	DS-CDC-HP with a positive charge and combined with a thermosensitive in situ gel might strengthen adherence to the corneal surface and prolong the ocular surface retention time to improve the bioavailability.
34576093	2	96	theme	dot	291:293	arg1	CDC-HP					295:300	The novel carbon dot CDC-HP	274:300	The novel carbon dot CDC-HP	274:300	The novel carbon dot CDC-HP was synthesized by the pyrolysis of hyaluronic acid and carboxymethyl chitosan through a one-step hydrothermal method and then embedded in a thermosensitive in situ gel of poloxamer 407 and poloxamer 188 through swelling loading.
34576093	9	97	theme	delivery	1427:1434	arg1	system					1436:1441	This composite ocular delivery system	1405:1441	This composite ocular delivery system	1405:1441	This composite ocular delivery system possesses potential applications in ocular imaging and drug delivery.
31961483	0	0	theme	melanoma	69:76	arg1	cells					78:82	human melanoma cells	63:82	human melanoma cells	63:82	Role of fucosyltransferase IV in the migration and invasion of human melanoma cells.
31961483	4	1	theme	profiling	697:705	arg1	composition					685:695	the composition profiling	681:705	the composition profiling of fucosylated N-glycans	681:730	In the present study, matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis revealed that the composition profiling of fucosylated N-glycans differed between high metastatic C8161 and low metastatic A375P cells.
31961483	4	2	theme	fucosylated	710:720	arg1	N-glycans					722:730	fucosylated N-glycans	710:730	fucosylated N-glycans	710:730	In the present study, matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis revealed that the composition profiling of fucosylated N-glycans differed between high metastatic C8161 and low metastatic A375P cells.
31961483	8	3	theme	cells	1156:1160	arg1	potential					1134:1142	the migration and invasion potential	1107:1142	the migration and invasion potential of melanoma cells	1107:1160	In addition, the migration and invasion potential of melanoma cells were decreased in C8161 and increased in A375P cells upon altering FUT4 expression levels by small interfering RNA or complementary DNA transfection.
31961483	1	4	theme	human	134:138	arg1	types					146:150	the most aggressive human tumor types	114:150	the most aggressive human tumor types	114:150	Malignant melanoma is one of the most aggressive human tumor types, mainly due to its high invasion capability, metastatic properties, and the absence of effective treatments.
31961483	8	5	theme	melanoma	1147:1154	arg1	cells					1156:1160	melanoma cells	1147:1160	melanoma cells	1147:1160	In addition, the migration and invasion potential of melanoma cells were decreased in C8161 and increased in A375P cells upon altering FUT4 expression levels by small interfering RNA or complementary DNA transfection.
31961483	0	6	theme	human	63:67	arg1	cells					78:82	human melanoma cells	63:82	human melanoma cells	63:82	Role of fucosyltransferase IV in the migration and invasion of human melanoma cells.
31961483	1	7	theme	tumor	140:144	arg1	types					146:150	the most aggressive human tumor types	114:150	the most aggressive human tumor types	114:150	Malignant melanoma is one of the most aggressive human tumor types, mainly due to its high invasion capability, metastatic properties, and the absence of effective treatments.
31961483	10	8	theme	therapeutic	1626:1636	arg1	target					1638:1643	a therapeutic target	1624:1643	a therapeutic target for melanoma	1624:1656	In conclusion, FUT4 is a novel biomarker and regulator of the migration and invasion of melanoma cells and may serve as a therapeutic target for melanoma.
31961483	10	8	theme	therapeutic	1626:1636	arg1	FUT4					1519:1522	FUT4	1519:1522	FUT4	1519:1522	In conclusion, FUT4 is a novel biomarker and regulator of the migration and invasion of melanoma cells and may serve as a therapeutic target for melanoma.
31961483	0	9	from	Role	0:3	arg1	invasion					51:58	invasion	51:58	invasion	51:58	Role of fucosyltransferase IV in the migration and invasion of human melanoma cells.
31961483	0	9	from	Role	0:3	arg1	migration					37:45	migration	37:45	migration	37:45	Role of fucosyltransferase IV in the migration and invasion of human melanoma cells.
31961483	4	10	theme	A375P	790:794	arg1	cells					796:800	low metastatic A375P cells	775:800	low metastatic A375P cells	775:800	In the present study, matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis revealed that the composition profiling of fucosylated N-glycans differed between high metastatic C8161 and low metastatic A375P cells.
31961483	8	11	dep	RNA	1273:1275	arg1	transfection					1298:1309	transfection	1298:1309	transfection	1298:1309	In addition, the migration and invasion potential of melanoma cells were decreased in C8161 and increased in A375P cells upon altering FUT4 expression levels by small interfering RNA or complementary DNA transfection.
31961483	0	12	theme	cells	78:82	arg1	invasion					51:58	invasion	51:58	invasion	51:58	Role of fucosyltransferase IV in the migration and invasion of human melanoma cells.
31961483	0	12	theme	cells	78:82	arg1	migration					37:45	migration	37:45	migration	37:45	Role of fucosyltransferase IV in the migration and invasion of human melanoma cells.
31961483	4	13	theme	metastatic	779:788	arg1	cells					796:800	low metastatic A375P cells	775:800	low metastatic A375P cells	775:800	In the present study, matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis revealed that the composition profiling of fucosylated N-glycans differed between high metastatic C8161 and low metastatic A375P cells.
31961483	4	14	theme	present	551:557	arg1	study					559:563	the present study	547:563	the present study	547:563	In the present study, matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis revealed that the composition profiling of fucosylated N-glycans differed between high metastatic C8161 and low metastatic A375P cells.
31961483	9	15	theme	melanoma	1466:1473	arg1	migration					1480:1488	melanoma cell migration	1466:1488	melanoma cell migration	1466:1488	Furthermore, regulating FUT4 expression markedly modulated the activity of the phosphoinositide-3-kinase/Akt (PI3K/Akt) signaling pathway, which affected melanoma cell migration and invasion.
31961483	10	16	theme	migration	1566:1574	arg1	FUT4					1519:1522	FUT4	1519:1522	FUT4	1519:1522	In conclusion, FUT4 is a novel biomarker and regulator of the migration and invasion of melanoma cells and may serve as a therapeutic target for melanoma.
31961483	10	16	theme	migration	1566:1574	arg1	regulator					1549:1557	a novel biomarker and regulator	1527:1557	regulator	1549:1557	In conclusion, FUT4 is a novel biomarker and regulator of the migration and invasion of melanoma cells and may serve as a therapeutic target for melanoma.
31961483	10	16	theme	migration	1566:1574	arg1	biomarker					1535:1543	a novel biomarker and regulator	1527:1557	biomarker	1535:1543	In conclusion, FUT4 is a novel biomarker and regulator of the migration and invasion of melanoma cells and may serve as a therapeutic target for melanoma.
31961483	1	17	theme	types	146:150	arg1	types					146:150	the most aggressive human tumor types	114:150	the most aggressive human tumor types	114:150	Malignant melanoma is one of the most aggressive human tumor types, mainly due to its high invasion capability, metastatic properties, and the absence of effective treatments.
31961483	1	17	theme	types	146:150	arg1	one					107:109	one	107:109	one	107:109	Malignant melanoma is one of the most aggressive human tumor types, mainly due to its high invasion capability, metastatic properties, and the absence of effective treatments.
31961483	8	18	theme	A375P	1203:1207	arg1	cells					1209:1213	A375P cells	1203:1213	A375P cells	1203:1213	In addition, the migration and invasion potential of melanoma cells were decreased in C8161 and increased in A375P cells upon altering FUT4 expression levels by small interfering RNA or complementary DNA transfection.
31961483	10	19	theme	novel	1529:1533	arg1	FUT4					1519:1522	FUT4	1519:1522	FUT4	1519:1522	In conclusion, FUT4 is a novel biomarker and regulator of the migration and invasion of melanoma cells and may serve as a therapeutic target for melanoma.
31961483	10	19	theme	novel	1529:1533	arg1	biomarker					1535:1543	a novel biomarker and regulator	1527:1557	biomarker	1535:1543	In conclusion, FUT4 is a novel biomarker and regulator of the migration and invasion of melanoma cells and may serve as a therapeutic target for melanoma.
31961483	7	20	theme	Altered	989:995	arg1	expression					1002:1011	Altered FUT4 expression	989:1011	Altered FUT4 expression	989:1011	Altered FUT4 expression was accompanied by a change in the migration and invasion capacity of the cells.
31961483	3	21	theme	fucosyltransferases	501:519	arg1	expression					487:496	the expression	483:496	the expression of fucosyltransferases (FUTs)	483:526	However, differences in glycosylation between high and low metastatic melanoma cells and how these regulate migration and invasion by altering the expression of fucosyltransferases (FUTs) remain unclear.
31961483	10	22	from	biomarker	1535:1543	arg1	conclusion					1507:1516	conclusion	1507:1516	conclusion	1507:1516	In conclusion, FUT4 is a novel biomarker and regulator of the migration and invasion of melanoma cells and may serve as a therapeutic target for melanoma.
31961483	3	23	theme	melanoma	410:417	arg1	cells					419:423	high and low metastatic melanoma cells	386:423	high and low metastatic melanoma cells	386:423	However, differences in glycosylation between high and low metastatic melanoma cells and how these regulate migration and invasion by altering the expression of fucosyltransferases (FUTs) remain unclear.
31961483	5	24	theme	FUT4	834:837	arg1	expression					839:848	FUT4 expression	834:848	FUT4 expression	834:848	Further analysis revealed that FUT4 expression was significantly increased in C8161 cells.
31961483	3	25	from	differences	349:359	arg1	glycosylation					364:376	glycosylation	364:376	glycosylation	364:376	However, differences in glycosylation between high and low metastatic melanoma cells and how these regulate migration and invasion by altering the expression of fucosyltransferases (FUTs) remain unclear.
31961483	7	26	theme	FUT4	997:1000	arg1	expression					1002:1011	Altered FUT4 expression	989:1011	Altered FUT4 expression	989:1011	Altered FUT4 expression was accompanied by a change in the migration and invasion capacity of the cells.
31961483	2	27	theme	pivotal	284:290	arg1	role					292:295	a pivotal role	282:295	a pivotal role	282:295	Glycosylation serves a pivotal role in the migration and invasion of melanoma.
31961483	10	28	theme	invasion	1580:1587	arg1	FUT4					1519:1522	FUT4	1519:1522	FUT4	1519:1522	In conclusion, FUT4 is a novel biomarker and regulator of the migration and invasion of melanoma cells and may serve as a therapeutic target for melanoma.
31961483	10	28	theme	invasion	1580:1587	arg1	regulator					1549:1557	a novel biomarker and regulator	1527:1557	regulator	1549:1557	In conclusion, FUT4 is a novel biomarker and regulator of the migration and invasion of melanoma cells and may serve as a therapeutic target for melanoma.
31961483	10	28	theme	invasion	1580:1587	arg1	biomarker					1535:1543	a novel biomarker and regulator	1527:1557	biomarker	1535:1543	In conclusion, FUT4 is a novel biomarker and regulator of the migration and invasion of melanoma cells and may serve as a therapeutic target for melanoma.
31961483	9	29	theme	phosphoinositide-3-kinase/Akt	1391:1419	arg1	pathway					1442:1448	the phosphoinositide-3-kinase/Akt (PI3K/Akt) signaling pathway	1387:1448	the phosphoinositide-3-kinase/Akt (PI3K/Akt) signaling pathway	1387:1448	Furthermore, regulating FUT4 expression markedly modulated the activity of the phosphoinositide-3-kinase/Akt (PI3K/Akt) signaling pathway, which affected melanoma cell migration and invasion.
31961483	10	30	theme	melanoma	1592:1599	arg1	cells					1601:1605	melanoma cells	1592:1605	melanoma cells	1592:1605	In conclusion, FUT4 is a novel biomarker and regulator of the migration and invasion of melanoma cells and may serve as a therapeutic target for melanoma.
31961483	4	31	theme	metastatic	754:763	arg1	C8161					765:769	high metastatic C8161	749:769	high metastatic C8161	749:769	In the present study, matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis revealed that the composition profiling of fucosylated N-glycans differed between high metastatic C8161 and low metastatic A375P cells.
31961483	3	32	theme	low	395:397	arg1	cells					419:423	high and low metastatic melanoma cells	386:423	high and low metastatic melanoma cells	386:423	However, differences in glycosylation between high and low metastatic melanoma cells and how these regulate migration and invasion by altering the expression of fucosyltransferases (FUTs) remain unclear.
31961483	8	33	theme	expression	1234:1243	arg1	levels					1245:1250	FUT4 expression levels	1229:1250	FUT4 expression levels	1229:1250	In addition, the migration and invasion potential of melanoma cells were decreased in C8161 and increased in A375P cells upon altering FUT4 expression levels by small interfering RNA or complementary DNA transfection.
31961483	1	34	theme	effective	239:247	arg1	treatments					249:258	effective treatments	239:258	effective treatments	239:258	Malignant melanoma is one of the most aggressive human tumor types, mainly due to its high invasion capability, metastatic properties, and the absence of effective treatments.
31961483	2	35	dep	migration	304:312	arg1	the					300:302	the	300:302	the	300:302	Glycosylation serves a pivotal role in the migration and invasion of melanoma.
31961483	0	36	theme	fucosyltransferase	8:25	arg1	Role					0:3	Role	0:3	Role of fucosyltransferase IV in the migration and invasion of human melanoma cells.	0:83	Role of fucosyltransferase IV in the migration and invasion of human melanoma cells.
31961483	1	37	theme	treatments	249:258	arg1	capability					185:194	its high invasion capability	167:194	its high invasion capability	167:194	Malignant melanoma is one of the most aggressive human tumor types, mainly due to its high invasion capability, metastatic properties, and the absence of effective treatments.
31961483	1	37	theme	treatments	249:258	arg1	properties					208:217	metastatic properties	197:217	metastatic properties	197:217	Malignant melanoma is one of the most aggressive human tumor types, mainly due to its high invasion capability, metastatic properties, and the absence of effective treatments.
31961483	1	37	theme	treatments	249:258	arg1	absence					228:234	the absence	224:234	the absence of effective treatments	224:258	Malignant melanoma is one of the most aggressive human tumor types, mainly due to its high invasion capability, metastatic properties, and the absence of effective treatments.
31961483	8	38	theme	migration	1111:1119	arg1	potential					1134:1142	the migration and invasion potential	1107:1142	the migration and invasion potential of melanoma cells	1107:1160	In addition, the migration and invasion potential of melanoma cells were decreased in C8161 and increased in A375P cells upon altering FUT4 expression levels by small interfering RNA or complementary DNA transfection.
31961483	4	39	theme	low	775:777	arg1	cells					796:800	low metastatic A375P cells	775:800	low metastatic A375P cells	775:800	In the present study, matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis revealed that the composition profiling of fucosylated N-glycans differed between high metastatic C8161 and low metastatic A375P cells.
31961483	4	40	theme	spectrometry	630:641	arg1	analysis					658:665	matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis	566:665	matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis	566:665	In the present study, matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis revealed that the composition profiling of fucosylated N-glycans differed between high metastatic C8161 and low metastatic A375P cells.
31961483	5	41	theme	Further	803:809	arg1	analysis					811:818	Further analysis	803:818	Further analysis	803:818	Further analysis revealed that FUT4 expression was significantly increased in C8161 cells.
31961483	9	42	theme	PI3K/Akt	1422:1429	arg1	pathway					1442:1448	the phosphoinositide-3-kinase/Akt (PI3K/Akt) signaling pathway	1387:1448	the phosphoinositide-3-kinase/Akt (PI3K/Akt) signaling pathway	1387:1448	Furthermore, regulating FUT4 expression markedly modulated the activity of the phosphoinositide-3-kinase/Akt (PI3K/Akt) signaling pathway, which affected melanoma cell migration and invasion.
31961483	5	43	theme	C8161	881:885	arg1	cells					887:891	C8161 cells	881:891	C8161 cells	881:891	Further analysis revealed that FUT4 expression was significantly increased in C8161 cells.
31961483	9	44	theme	FUT4	1336:1339	arg1	expression					1341:1350	regulating FUT4 expression	1325:1350	regulating FUT4 expression	1325:1350	Furthermore, regulating FUT4 expression markedly modulated the activity of the phosphoinositide-3-kinase/Akt (PI3K/Akt) signaling pathway, which affected melanoma cell migration and invasion.
31961483	8	45	theme	complementary	1280:1292	arg1	DNA					1294:1296	complementary DNA	1280:1296	complementary DNA	1280:1296	In addition, the migration and invasion potential of melanoma cells were decreased in C8161 and increased in A375P cells upon altering FUT4 expression levels by small interfering RNA or complementary DNA transfection.
31961483	1	46	theme	high	171:174	arg1	capability					185:194	its high invasion capability	167:194	its high invasion capability	167:194	Malignant melanoma is one of the most aggressive human tumor types, mainly due to its high invasion capability, metastatic properties, and the absence of effective treatments.
31961483	3	47	theme	high	386:389	arg1	cells					419:423	high and low metastatic melanoma cells	386:423	high and low metastatic melanoma cells	386:423	However, differences in glycosylation between high and low metastatic melanoma cells and how these regulate migration and invasion by altering the expression of fucosyltransferases (FUTs) remain unclear.
31961483	8	48	theme	invasion	1125:1132	arg1	potential					1134:1142	the migration and invasion potential	1107:1142	the migration and invasion potential of melanoma cells	1107:1160	In addition, the migration and invasion potential of melanoma cells were decreased in C8161 and increased in A375P cells upon altering FUT4 expression levels by small interfering RNA or complementary DNA transfection.
31961483	6	49	theme	Melanoma	894:901	arg1	arrays					910:915	Melanoma tissue arrays	894:915	Melanoma tissue arrays	894:915	Melanoma tissue arrays further demonstrated that FUT4 was overexpressed in metastatic samples.
31961483	0	50	dep	migration	37:45	arg1	the					33:35	the	33:35	the	33:35	Role of fucosyltransferase IV in the migration and invasion of human melanoma cells.
31961483	7	51	theme	invasion	1062:1069	arg1	capacity					1071:1078	the migration and invasion capacity	1044:1078	the migration and invasion capacity of the cells	1044:1091	Altered FUT4 expression was accompanied by a change in the migration and invasion capacity of the cells.
31961483	1	52	theme	invasion	176:183	arg1	capability					185:194	its high invasion capability	167:194	its high invasion capability	167:194	Malignant melanoma is one of the most aggressive human tumor types, mainly due to its high invasion capability, metastatic properties, and the absence of effective treatments.
31961483	8	53	theme	interfering	1261:1271	arg1	RNA					1273:1275	small interfering RNA	1255:1275	small interfering RNA	1255:1275	In addition, the migration and invasion potential of melanoma cells were decreased in C8161 and increased in A375P cells upon altering FUT4 expression levels by small interfering RNA or complementary DNA transfection.
31961483	9	54	theme	signaling	1432:1440	arg1	pathway					1442:1448	the phosphoinositide-3-kinase/Akt (PI3K/Akt) signaling pathway	1387:1448	the phosphoinositide-3-kinase/Akt (PI3K/Akt) signaling pathway	1387:1448	Furthermore, regulating FUT4 expression markedly modulated the activity of the phosphoinositide-3-kinase/Akt (PI3K/Akt) signaling pathway, which affected melanoma cell migration and invasion.
31961483	7	55	theme	cells	1087:1091	arg1	capacity					1071:1078	the migration and invasion capacity	1044:1078	the migration and invasion capacity of the cells	1044:1091	Altered FUT4 expression was accompanied by a change in the migration and invasion capacity of the cells.
31961483	3	56	theme	metastatic	399:408	arg1	cells					419:423	high and low metastatic melanoma cells	386:423	high and low metastatic melanoma cells	386:423	However, differences in glycosylation between high and low metastatic melanoma cells and how these regulate migration and invasion by altering the expression of fucosyltransferases (FUTs) remain unclear.
31961483	8	57	theme	small	1255:1259	arg1	RNA					1273:1275	small interfering RNA	1255:1275	small interfering RNA	1255:1275	In addition, the migration and invasion potential of melanoma cells were decreased in C8161 and increased in A375P cells upon altering FUT4 expression levels by small interfering RNA or complementary DNA transfection.
31961483	9	58	theme	pathway	1442:1448	arg1	activity					1375:1382	the activity	1371:1382	the activity of the phosphoinositide-3-kinase/Akt (PI3K/Akt) signaling pathway, which affected melanoma cell migration and invasion	1371:1501	Furthermore, regulating FUT4 expression markedly modulated the activity of the phosphoinositide-3-kinase/Akt (PI3K/Akt) signaling pathway, which affected melanoma cell migration and invasion.
31961483	9	59	theme	regulating	1325:1334	arg1	expression					1341:1350	regulating FUT4 expression	1325:1350	regulating FUT4 expression	1325:1350	Furthermore, regulating FUT4 expression markedly modulated the activity of the phosphoinositide-3-kinase/Akt (PI3K/Akt) signaling pathway, which affected melanoma cell migration and invasion.
31961483	10	60	theme	cells	1601:1605	arg1	invasion					1580:1587	invasion	1580:1587	invasion	1580:1587	In conclusion, FUT4 is a novel biomarker and regulator of the migration and invasion of melanoma cells and may serve as a therapeutic target for melanoma.
31961483	10	60	theme	cells	1601:1605	arg1	migration					1566:1574	migration	1566:1574	migration	1566:1574	In conclusion, FUT4 is a novel biomarker and regulator of the migration and invasion of melanoma cells and may serve as a therapeutic target for melanoma.
31961483	7	61	theme	migration	1048:1056	arg1	capacity					1071:1078	the migration and invasion capacity	1044:1078	the migration and invasion capacity of the cells	1044:1091	Altered FUT4 expression was accompanied by a change in the migration and invasion capacity of the cells.
31961483	4	62	theme	N-glycans	722:730	arg1	composition					685:695	the composition profiling	681:705	the composition profiling of fucosylated N-glycans	681:730	In the present study, matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis revealed that the composition profiling of fucosylated N-glycans differed between high metastatic C8161 and low metastatic A375P cells.
31961483	10	63	from	regulator	1549:1557	arg1	conclusion					1507:1516	conclusion	1507:1516	conclusion	1507:1516	In conclusion, FUT4 is a novel biomarker and regulator of the migration and invasion of melanoma cells and may serve as a therapeutic target for melanoma.
31961483	4	64	theme	high	749:752	arg1	C8161					765:769	high metastatic C8161	749:769	high metastatic C8161	749:769	In the present study, matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis revealed that the composition profiling of fucosylated N-glycans differed between high metastatic C8161 and low metastatic A375P cells.
31961483	1	65	theme	Malignant	85:93	arg1	melanoma					95:102	Malignant melanoma	85:102	Malignant melanoma	85:102	Malignant melanoma is one of the most aggressive human tumor types, mainly due to its high invasion capability, metastatic properties, and the absence of effective treatments.
31961483	4	66	theme	matrix-assisted	566:580	arg1	MALDI-TOF-MS					644:655	MALDI-TOF-MS	644:655	MALDI-TOF-MS	644:655	In the present study, matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis revealed that the composition profiling of fucosylated N-glycans differed between high metastatic C8161 and low metastatic A375P cells.
31961483	4	66	theme	matrix-assisted	566:580	arg1	spectrometry					630:641	matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry	566:641	matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis	566:665	In the present study, matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis revealed that the composition profiling of fucosylated N-glycans differed between high metastatic C8161 and low metastatic A375P cells.
31961483	4	67	gly	fucosylated	710:720	arg1	N-glycans					722:730	fucosylated N-glycans	710:730	fucosylated N-glycans	710:730	In the present study, matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis revealed that the composition profiling of fucosylated N-glycans differed between high metastatic C8161 and low metastatic A375P cells.
31961483	6	68	theme	tissue	903:908	arg1	arrays					910:915	Melanoma tissue arrays	894:915	Melanoma tissue arrays	894:915	Melanoma tissue arrays further demonstrated that FUT4 was overexpressed in metastatic samples.
31961483	4	69	theme	desorption/ionization-time-of-flight-mass	588:628	arg1	MALDI-TOF-MS					644:655	MALDI-TOF-MS	644:655	MALDI-TOF-MS	644:655	In the present study, matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis revealed that the composition profiling of fucosylated N-glycans differed between high metastatic C8161 and low metastatic A375P cells.
31961483	4	69	theme	desorption/ionization-time-of-flight-mass	588:628	arg1	spectrometry					630:641	matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry	566:641	matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis	566:665	In the present study, matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis revealed that the composition profiling of fucosylated N-glycans differed between high metastatic C8161 and low metastatic A375P cells.
31961483	2	70	theme	melanoma	330:337	arg1	invasion					318:325	invasion	318:325	invasion	318:325	Glycosylation serves a pivotal role in the migration and invasion of melanoma.
31961483	2	70	theme	melanoma	330:337	arg1	migration					304:312	migration	304:312	migration	304:312	Glycosylation serves a pivotal role in the migration and invasion of melanoma.
31961483	7	71	from	change	1034:1039	arg1	capacity					1071:1078	the migration and invasion capacity	1044:1078	the migration and invasion capacity of the cells	1044:1091	Altered FUT4 expression was accompanied by a change in the migration and invasion capacity of the cells.
31961483	1	72	theme	metastatic	197:206	arg1	properties					208:217	metastatic properties	197:217	metastatic properties	197:217	Malignant melanoma is one of the most aggressive human tumor types, mainly due to its high invasion capability, metastatic properties, and the absence of effective treatments.
31961483	10	73	dep	migration	1566:1574	arg1	the					1562:1564	the	1562:1564	the	1562:1564	In conclusion, FUT4 is a novel biomarker and regulator of the migration and invasion of melanoma cells and may serve as a therapeutic target for melanoma.
31961483	6	74	theme	metastatic	969:978	arg1	samples					980:986	metastatic samples	969:986	metastatic samples	969:986	Melanoma tissue arrays further demonstrated that FUT4 was overexpressed in metastatic samples.
31961483	8	75	theme	FUT4	1229:1232	arg1	levels					1245:1250	FUT4 expression levels	1229:1250	FUT4 expression levels	1229:1250	In addition, the migration and invasion potential of melanoma cells were decreased in C8161 and increased in A375P cells upon altering FUT4 expression levels by small interfering RNA or complementary DNA transfection.
31961483	1	76	theme	aggressive	123:132	arg1	types					146:150	the most aggressive human tumor types	114:150	the most aggressive human tumor types	114:150	Malignant melanoma is one of the most aggressive human tumor types, mainly due to its high invasion capability, metastatic properties, and the absence of effective treatments.
31961483	9	77	theme	cell	1475:1478	arg1	migration					1480:1488	melanoma cell migration	1466:1488	melanoma cell migration	1466:1488	Furthermore, regulating FUT4 expression markedly modulated the activity of the phosphoinositide-3-kinase/Akt (PI3K/Akt) signaling pathway, which affected melanoma cell migration and invasion.
31961483	4	78	theme	laser	582:586	arg1	MALDI-TOF-MS					644:655	MALDI-TOF-MS	644:655	MALDI-TOF-MS	644:655	In the present study, matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis revealed that the composition profiling of fucosylated N-glycans differed between high metastatic C8161 and low metastatic A375P cells.
31961483	4	78	theme	laser	582:586	arg1	spectrometry					630:641	matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry	566:641	matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis	566:665	In the present study, matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis revealed that the composition profiling of fucosylated N-glycans differed between high metastatic C8161 and low metastatic A375P cells.
34139243	0	0	theme	temperature	148:158	arg1	Effect					131:136	Effect	131:136	Facile preparation of layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac: Effect of pH and temperature on release rate.	0:175	Facile preparation of layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac: Effect of pH and temperature on release rate.
34139243	0	1	theme	beads	62:66	arg1	preparation					7:17	Facile preparation	0:17	Facile preparation of layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac: Effect of pH and temperature on release rate.	0:175	Facile preparation of layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac: Effect of pH and temperature on release rate.
34139243	1	2	theme	alginate	223:230	arg1	beads					232:236	alginate beads	223:236	alginate beads encapsulating layered double hydroxide (LDH) intercalated with diclofenac sodium as drug delivery systems	223:342	This paper concerns the facile preparation of alginate beads encapsulating layered double hydroxide (LDH) intercalated with diclofenac sodium as drug delivery systems.
34139243	1	3	theme	drug	322:325	arg1	systems					336:342	drug delivery systems	322:342	drug delivery systems	322:342	This paper concerns the facile preparation of alginate beads encapsulating layered double hydroxide (LDH) intercalated with diclofenac sodium as drug delivery systems.
34139243	0	4	theme	release	163:169	arg1	rate					171:174	release rate	163:174	release rate	163:174	Facile preparation of layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac: Effect of pH and temperature on release rate.
34139243	1	5	theme	beads	232:236	arg1	preparation					208:218	the facile preparation	197:218	the facile preparation of alginate beads encapsulating layered double hydroxide (LDH) intercalated with diclofenac sodium as drug delivery systems	197:342	This paper concerns the facile preparation of alginate beads encapsulating layered double hydroxide (LDH) intercalated with diclofenac sodium as drug delivery systems.
34139243	0	6	theme	sustainable	71:81	arg1	system					83:88	sustainable system	71:88	sustainable system for the triggered release of diclofenac	71:128	Facile preparation of layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac: Effect of pH and temperature on release rate.
34139243	1	7	theme	delivery	327:334	arg1	systems					336:342	drug delivery systems	322:342	drug delivery systems	322:342	This paper concerns the facile preparation of alginate beads encapsulating layered double hydroxide (LDH) intercalated with diclofenac sodium as drug delivery systems.
34139243	6	8	theme	drug	971:974	arg1	rate					984:987	a triggered drug release rate	959:987	a triggered drug release rate	959:987	The diclofenac release behavior appeared to be affected by the presence of LDH, the pH of release medium and the temperature allowing for fabricating a sustainable composite characterized by a triggered drug release rate.
34139243	6	9	theme	medium	866:871	arg1	temperature					881:891	the temperature	877:891	the temperature	877:891	The diclofenac release behavior appeared to be affected by the presence of LDH, the pH of release medium and the temperature allowing for fabricating a sustainable composite characterized by a triggered drug release rate.
34139243	6	9	theme	medium	866:871	arg1	pH					852:853	the pH	848:853	the pH of release medium	848:871	The diclofenac release behavior appeared to be affected by the presence of LDH, the pH of release medium and the temperature allowing for fabricating a sustainable composite characterized by a triggered drug release rate.
34139243	7	10	theme	pH	1087:1088	arg1	diffusion					1044:1052	the drug diffusion	1035:1052	the drug diffusion	1035:1052	Finally, empirical relationships correlating the drug diffusion as a function of temperature and pH were extrapolated.
34139243	7	10	theme	pH	1087:1088	arg1	function					1059:1066	a function	1057:1066	a function of temperature and pH	1057:1088	Finally, empirical relationships correlating the drug diffusion as a function of temperature and pH were extrapolated.
34139243	3	11	dep	Composites	454:463	arg1	crosslinked					500:510	crosslinked	500:510	were ionotropically crosslinked in CaCl2 solution at 4 °C. Thermal and barrier properties	480:568	Composites hydrogel beads were ionotropically crosslinked in CaCl2 solution at 4 °C. Thermal and barrier properties were evaluated and correlated with the presence of the inorganic phase.
34139243	4	12	theme	Swelling	642:649	arg1	behavior					651:658	Swelling behavior	642:658	Swelling behavior	642:658	Swelling behavior was investigated over time.
34139243	6	13	theme	release	858:864	arg1	medium					866:871	release medium	858:871	release medium	858:871	The diclofenac release behavior appeared to be affected by the presence of LDH, the pH of release medium and the temperature allowing for fabricating a sustainable composite characterized by a triggered drug release rate.
34139243	3	14	theme	CaCl2	515:519	arg1	solution					521:528	CaCl2 solution	515:528	CaCl2 solution	515:528	Composites hydrogel beads were ionotropically crosslinked in CaCl2 solution at 4 °C. Thermal and barrier properties were evaluated and correlated with the presence of the inorganic phase.
34139243	7	15	theme	temperature	1071:1081	arg1	diffusion					1044:1052	the drug diffusion	1035:1052	the drug diffusion	1035:1052	Finally, empirical relationships correlating the drug diffusion as a function of temperature and pH were extrapolated.
34139243	7	15	theme	temperature	1071:1081	arg1	function					1059:1066	a function	1057:1066	a function of temperature and pH	1057:1088	Finally, empirical relationships correlating the drug diffusion as a function of temperature and pH were extrapolated.
34139243	6	16	theme	release	783:789	arg1	behavior					791:798	The diclofenac release behavior	768:798	The diclofenac release behavior	768:798	The diclofenac release behavior appeared to be affected by the presence of LDH, the pH of release medium and the temperature allowing for fabricating a sustainable composite characterized by a triggered drug release rate.
34139243	0	17	theme	Facile	0:5	arg1	preparation					7:17	Facile preparation	0:17	Facile preparation of layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac: Effect of pH and temperature on release rate.	0:175	Facile preparation of layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac: Effect of pH and temperature on release rate.
34139243	1	18	theme	layered	252:258	arg1	hydroxide					267:275	layered double hydroxide	252:275	layered double hydroxide (LDH) intercalated with diclofenac sodium as drug delivery systems	252:342	This paper concerns the facile preparation of alginate beads encapsulating layered double hydroxide (LDH) intercalated with diclofenac sodium as drug delivery systems.
34139243	1	18	theme	layered	252:258	arg1	LDH					278:280	LDH	278:280	LDH	278:280	This paper concerns the facile preparation of alginate beads encapsulating layered double hydroxide (LDH) intercalated with diclofenac sodium as drug delivery systems.
34139243	6	19	theme	diclofenac	772:781	arg1	behavior					791:798	The diclofenac release behavior	768:798	The diclofenac release behavior	768:798	The diclofenac release behavior appeared to be affected by the presence of LDH, the pH of release medium and the temperature allowing for fabricating a sustainable composite characterized by a triggered drug release rate.
34139243	3	20	theme	barrier	551:557	arg1	properties					559:568	barrier properties	551:568	barrier properties	551:568	Composites hydrogel beads were ionotropically crosslinked in CaCl2 solution at 4 °C. Thermal and barrier properties were evaluated and correlated with the presence of the inorganic phase.
34139243	0	21	theme	layered	22:28	arg1	beads					62:66	layered double hydroxide (LDH)-alginate beads	22:66	layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac	22:128	Facile preparation of layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac: Effect of pH and temperature on release rate.
34139243	1	22	theme	double	260:265	arg1	hydroxide					267:275	layered double hydroxide	252:275	layered double hydroxide (LDH) intercalated with diclofenac sodium as drug delivery systems	252:342	This paper concerns the facile preparation of alginate beads encapsulating layered double hydroxide (LDH) intercalated with diclofenac sodium as drug delivery systems.
34139243	1	22	theme	double	260:265	arg1	LDH					278:280	LDH	278:280	LDH	278:280	This paper concerns the facile preparation of alginate beads encapsulating layered double hydroxide (LDH) intercalated with diclofenac sodium as drug delivery systems.
34139243	5	23	theme	Release	688:694	arg1	kinetics					696:703	Release kinetics	688:703	Release kinetics of diclofenac at different pH and temperatures	688:750	Release kinetics of diclofenac at different pH and temperatures were evaluated.
34139243	2	24	theme	alginate	391:398	arg1	beads					400:404	alginate beads	391:404	alginate beads loaded with free diclofenac	391:432	To better evaluate the effect of LDH carrier, alginate beads loaded with free diclofenac were also prepared.
34139243	0	25	theme	triggered	98:106	arg1	release					108:114	the triggered release	94:114	the triggered release of diclofenac	94:128	Facile preparation of layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac: Effect of pH and temperature on release rate.
34139243	3	26	theme	inorganic	625:633	arg1	phase					635:639	the inorganic phase	621:639	the inorganic phase	621:639	Composites hydrogel beads were ionotropically crosslinked in CaCl2 solution at 4 °C. Thermal and barrier properties were evaluated and correlated with the presence of the inorganic phase.
34139243	3	27	theme	Thermal	539:545	arg1	4 °C.					533:537	4 °C. Thermal	533:545	4 °C. Thermal	533:545	Composites hydrogel beads were ionotropically crosslinked in CaCl2 solution at 4 °C. Thermal and barrier properties were evaluated and correlated with the presence of the inorganic phase.
34139243	6	28	dep	affected	815:822	arg1	allowing					893:900	allowing	893:900	allowing for fabricating a sustainable composite characterized by a triggered drug release rate	893:987	The diclofenac release behavior appeared to be affected by the presence of LDH, the pH of release medium and the temperature allowing for fabricating a sustainable composite characterized by a triggered drug release rate.
34139243	2	29	theme	carrier	382:388	arg1	effect					368:373	the effect	364:373	the effect of LDH carrier	364:388	To better evaluate the effect of LDH carrier, alginate beads loaded with free diclofenac were also prepared.
34139243	0	30	theme	hydroxide	37:45	arg1	beads					62:66	layered double hydroxide (LDH)-alginate beads	22:66	layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac	22:128	Facile preparation of layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac: Effect of pH and temperature on release rate.
34139243	0	31	theme	diclofenac	119:128	arg1	release					108:114	the triggered release	94:114	the triggered release of diclofenac	94:128	Facile preparation of layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac: Effect of pH and temperature on release rate.
34139243	3	32	theme	phase	635:639	arg1	presence					609:616	the presence	605:616	the presence of the inorganic phase	605:639	Composites hydrogel beads were ionotropically crosslinked in CaCl2 solution at 4 °C. Thermal and barrier properties were evaluated and correlated with the presence of the inorganic phase.
34139243	2	33	theme	LDH	378:380	arg1	carrier					382:388	LDH carrier	378:388	LDH carrier	378:388	To better evaluate the effect of LDH carrier, alginate beads loaded with free diclofenac were also prepared.
34139243	0	34	theme	double	30:35	arg1	hydroxide					37:45	double hydroxide	30:45	layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac	22:128	Facile preparation of layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac: Effect of pH and temperature on release rate.
34139243	0	34	theme	double	30:35	arg1	LDH					48:50	LDH	48:50	LDH	48:50	Facile preparation of layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac: Effect of pH and temperature on release rate.
34139243	5	35	theme	diclofenac	708:717	arg1	kinetics					696:703	Release kinetics	688:703	Release kinetics of diclofenac at different pH and temperatures	688:750	Release kinetics of diclofenac at different pH and temperatures were evaluated.
34139243	3	36	attach	crosslinked	500:510	arg3	properties					559:568	barrier properties	551:568	barrier properties	551:568	Composites hydrogel beads were ionotropically crosslinked in CaCl2 solution at 4 °C. Thermal and barrier properties were evaluated and correlated with the presence of the inorganic phase.
34139243	3	36	attach	crosslinked	500:510	arg3	solution					521:528	CaCl2 solution	515:528	CaCl2 solution	515:528	Composites hydrogel beads were ionotropically crosslinked in CaCl2 solution at 4 °C. Thermal and barrier properties were evaluated and correlated with the presence of the inorganic phase.
34139243	3	36	attach	crosslinked	500:510	arg3	4 °C.					533:537	4 °C. Thermal	533:545	4 °C. Thermal	533:545	Composites hydrogel beads were ionotropically crosslinked in CaCl2 solution at 4 °C. Thermal and barrier properties were evaluated and correlated with the presence of the inorganic phase.
34139243	6	37	theme	sustainable	920:930	arg1	composite					932:940	a sustainable composite	918:940	a sustainable composite characterized by a triggered drug release rate	918:987	The diclofenac release behavior appeared to be affected by the presence of LDH, the pH of release medium and the temperature allowing for fabricating a sustainable composite characterized by a triggered drug release rate.
34139243	7	38	theme	drug	1039:1042	arg1	diffusion					1044:1052	the drug diffusion	1035:1052	the drug diffusion	1035:1052	Finally, empirical relationships correlating the drug diffusion as a function of temperature and pH were extrapolated.
34139243	7	38	theme	drug	1039:1042	arg1	function					1059:1066	a function	1057:1066	a function of temperature and pH	1057:1088	Finally, empirical relationships correlating the drug diffusion as a function of temperature and pH were extrapolated.
34139243	6	39	theme	LDH	843:845	arg1	presence					831:838	the presence	827:838	the presence of LDH	827:845	The diclofenac release behavior appeared to be affected by the presence of LDH, the pH of release medium and the temperature allowing for fabricating a sustainable composite characterized by a triggered drug release rate.
34139243	2	40	theme	free	418:421	arg1	diclofenac					423:432	free diclofenac	418:432	free diclofenac	418:432	To better evaluate the effect of LDH carrier, alginate beads loaded with free diclofenac were also prepared.
34139243	3	41	theme	hydrogel	465:472	arg1	beads					474:478	hydrogel beads	465:478	hydrogel beads	465:478	Composites hydrogel beads were ionotropically crosslinked in CaCl2 solution at 4 °C. Thermal and barrier properties were evaluated and correlated with the presence of the inorganic phase.
34139243	0	42	theme	pH	141:142	arg1	Effect					131:136	Effect	131:136	Facile preparation of layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac: Effect of pH and temperature on release rate.	0:175	Facile preparation of layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac: Effect of pH and temperature on release rate.
34139243	5	43	from	pH	732:733	arg1	kinetics					696:703	Release kinetics	688:703	Release kinetics of diclofenac at different pH and temperatures	688:750	Release kinetics of diclofenac at different pH and temperatures were evaluated.
34139243	6	44	theme	release	976:982	arg1	rate					984:987	a triggered drug release rate	959:987	a triggered drug release rate	959:987	The diclofenac release behavior appeared to be affected by the presence of LDH, the pH of release medium and the temperature allowing for fabricating a sustainable composite characterized by a triggered drug release rate.
34139243	5	45	from	temperatures	739:750	arg1	kinetics					696:703	Release kinetics	688:703	Release kinetics of diclofenac at different pH and temperatures	688:750	Release kinetics of diclofenac at different pH and temperatures were evaluated.
34139243	7	46	theme	empirical	999:1007	arg1	relationships					1009:1021	empirical relationships	999:1021	empirical relationships correlating the drug diffusion as a function of temperature and pH	999:1088	Finally, empirical relationships correlating the drug diffusion as a function of temperature and pH were extrapolated.
34139243	0	47	theme	-alginate	52:60	arg1	beads					62:66	layered double hydroxide (LDH)-alginate beads	22:66	layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac	22:128	Facile preparation of layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac: Effect of pH and temperature on release rate.
34139243	0	48	dep	preparation	7:17	arg1	Effect					131:136	Effect	131:136	Facile preparation of layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac: Effect of pH and temperature on release rate.	0:175	Facile preparation of layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac: Effect of pH and temperature on release rate.
34139243	1	49	theme	facile	201:206	arg1	preparation					208:218	the facile preparation	197:218	the facile preparation of alginate beads encapsulating layered double hydroxide (LDH) intercalated with diclofenac sodium as drug delivery systems	197:342	This paper concerns the facile preparation of alginate beads encapsulating layered double hydroxide (LDH) intercalated with diclofenac sodium as drug delivery systems.
34139243	0	50	from	Effect	131:136	arg1	rate					171:174	release rate	163:174	release rate	163:174	Facile preparation of layered double hydroxide (LDH)-alginate beads as sustainable system for the triggered release of diclofenac: Effect of pH and temperature on release rate.
34139243	1	51	theme	diclofenac	301:310	arg1	sodium					312:317	diclofenac sodium	301:317	diclofenac sodium	301:317	This paper concerns the facile preparation of alginate beads encapsulating layered double hydroxide (LDH) intercalated with diclofenac sodium as drug delivery systems.
34139243	6	52	theme	triggered	961:969	arg1	rate					984:987	a triggered drug release rate	959:987	a triggered drug release rate	959:987	The diclofenac release behavior appeared to be affected by the presence of LDH, the pH of release medium and the temperature allowing for fabricating a sustainable composite characterized by a triggered drug release rate.
34139243	5	53	theme	different	722:730	arg1	pH					732:733	different pH	722:733	different pH	722:733	Release kinetics of diclofenac at different pH and temperatures were evaluated.
32283375	0	0	theme	infant	90:95	arg1	formula					97:103	infant formula	90:103	infant formula	90:103	Inter-relationship between lactose crystallization and surface free fat during storage of infant formula.
32283375	1	1	theme	intact	314:319	arg1	protein					343:349	hydrolyzed and intact (non-hydrolyzed) whey protein	299:349	protein	343:349	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	1	2	from	composition	178:188	arg1	properties					254:263	physico-chemical properties	237:263	physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition	237:370	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	1	3	dep	temperatures	397:408	arg1	conditions					446:455	conditions	446:455	conditions	446:455	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	4	4	theme	fats	699:702	arg1	proportion					673:682	higher proportion	666:682	higher proportion of unsaturated fats in SFF of powders with large lactose crystals and vice-versa	666:763	IF composition influenced LC and caused selective migration of triglycerides, resulting in higher proportion of unsaturated fats in SFF of powders with large lactose crystals and vice-versa.
32283375	5	5	theme	reduced	860:866	arg1	wettability					868:878	reduced wettability	860:878	reduced wettability of powders	860:889	Increase in SFF with increased proportion of saturated fats in their composition, resulted in reduced wettability of powders.
32283375	1	6	theme	non-hydrolyzed	322:335	arg1	protein					343:349	hydrolyzed and intact (non-hydrolyzed) whey protein	299:349	protein	343:349	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	1	7	from	amount	167:172	arg1	properties					254:263	physico-chemical properties	237:263	physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition	237:370	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	1	8	theme	physico-chemical	237:252	arg1	properties					254:263	physico-chemical properties	237:263	physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition	237:370	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	0	9	theme	formula	97:103	arg1	storage					79:85	storage	79:85	storage of infant formula	79:103	Inter-relationship between lactose crystallization and surface free fat during storage of infant formula.
32283375	4	10	theme	powders	714:720	arg1	SFF					707:709	SFF	707:709	SFF of powders with large lactose crystals and vice-versa	707:763	IF composition influenced LC and caused selective migration of triglycerides, resulting in higher proportion of unsaturated fats in SFF of powders with large lactose crystals and vice-versa.
32283375	1	11	theme	whey	338:341	arg1	protein					343:349	hydrolyzed and intact (non-hydrolyzed) whey protein	299:349	protein	343:349	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	6	12	theme	IF	1037:1038	arg1	powders					1040:1046	IF powders	1037:1046	IF powders	1037:1046	Overall, IF composition affects LC which influences the amount and type of fat migration to particle surface resulting in varying wettability of IF powders.
32283375	4	13	theme	lactose	733:739	arg1	crystals					741:748	large lactose crystals	727:748	large lactose crystals	727:748	IF composition influenced LC and caused selective migration of triglycerides, resulting in higher proportion of unsaturated fats in SFF of powders with large lactose crystals and vice-versa.
32283375	4	14	theme	selective	615:623	arg1	migration					625:633	selective migration	615:633	selective migration of triglycerides	615:650	IF composition influenced LC and caused selective migration of triglycerides, resulting in higher proportion of unsaturated fats in SFF of powders with large lactose crystals and vice-versa.
32283375	5	15	from	Increase	766:773	arg1	SFF					778:780	SFF	778:780	SFF with increased proportion of saturated fats	778:824	Increase in SFF with increased proportion of saturated fats in their composition, resulted in reduced wettability of powders.
32283375	5	15	from	Increase	766:773	arg1	composition					835:845	their composition	829:845	their composition	829:845	Increase in SFF with increased proportion of saturated fats in their composition, resulted in reduced wettability of powders.
32283375	4	16	theme	large	727:731	arg1	crystals					741:748	large lactose crystals	727:748	large lactose crystals	727:748	IF composition influenced LC and caused selective migration of triglycerides, resulting in higher proportion of unsaturated fats in SFF of powders with large lactose crystals and vice-versa.
32283375	1	17	from	protein	343:349	arg1	composition					360:370	their composition	354:370	their composition	354:370	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	4	18	from	SFF	707:709	arg1	proportion					673:682	higher proportion	666:682	higher proportion of unsaturated fats in SFF of powders with large lactose crystals and vice-versa	666:763	IF composition influenced LC and caused selective migration of triglycerides, resulting in higher proportion of unsaturated fats in SFF of powders with large lactose crystals and vice-versa.
32283375	5	19	with	SFF	778:780	arg1	proportion					797:806	increased proportion	787:806	increased proportion of saturated fats	787:824	Increase in SFF with increased proportion of saturated fats in their composition, resulted in reduced wettability of powders.
32283375	1	20	contain	containing	288:297	arg1	formula					275:281	infant formula	268:281	infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition	268:370	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	1	20	contain	containing	288:297	arg2	protein					343:349	hydrolyzed and intact (non-hydrolyzed) whey protein	299:349	protein	343:349	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	1	21	theme	surface	193:199	arg1	fat					206:208	surface free fat	193:208	surface free fat (SFF)	193:214	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	1	21	theme	surface	193:199	arg1	SFF					211:213	SFF	211:213	SFF	211:213	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	1	22	theme	infant	268:273	arg1	formula					275:281	infant formula	268:281	infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition	268:370	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	1	23	from	crystallization	141:155	arg1	properties					254:263	physico-chemical properties	237:263	physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition	237:370	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	1	24	theme	free	201:204	arg1	fat					206:208	surface free fat	193:208	surface free fat (SFF)	193:214	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	1	24	theme	free	201:204	arg1	SFF					211:213	SFF	211:213	SFF	211:213	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	0	25	theme	lactose	27:33	arg1	crystallization					35:49	lactose crystallization	27:49	lactose crystallization	27:49	Inter-relationship between lactose crystallization and surface free fat during storage of infant formula.
32283375	1	26	theme	formula	275:281	arg1	properties					254:263	physico-chemical properties	237:263	physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition	237:370	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	6	27	theme	powders	1040:1046	arg1	wettability					1022:1032	wettability	1022:1032	wettability of IF powders	1022:1046	Overall, IF composition affects LC which influences the amount and type of fat migration to particle surface resulting in varying wettability of IF powders.
32283375	1	28	theme	fat	206:208	arg1	amount					167:172	the amount	163:172	the amount	163:172	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	1	28	theme	fat	206:208	arg1	fat					206:208	surface free fat	193:208	surface free fat (SFF)	193:214	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	1	28	theme	fat	206:208	arg1	crystallization					141:155	lactose crystallization	133:155	lactose crystallization (LC)	133:160	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	1	28	theme	fat	206:208	arg1	effect					227:232	their effect	221:232	their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition	221:370	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	1	28	theme	fat	206:208	arg1	SFF					211:213	SFF	211:213	SFF	211:213	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	1	28	theme	fat	206:208	arg1	LC					158:159	LC	158:159	LC	158:159	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	1	28	theme	fat	206:208	arg1	composition					178:188	composition	178:188	composition	178:188	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	0	29	theme	surface	55:61	arg1	fat					68:70	surface free fat	55:70	surface free fat	55:70	Inter-relationship between lactose crystallization and surface free fat during storage of infant formula.
32283375	4	30	with	powders	714:720	arg1	vice-versa					754:763	vice-versa	754:763	vice-versa	754:763	IF composition influenced LC and caused selective migration of triglycerides, resulting in higher proportion of unsaturated fats in SFF of powders with large lactose crystals and vice-versa.
32283375	4	30	with	powders	714:720	arg1	crystals					741:748	large lactose crystals	727:748	large lactose crystals	727:748	IF composition influenced LC and caused selective migration of triglycerides, resulting in higher proportion of unsaturated fats in SFF of powders with large lactose crystals and vice-versa.
32283375	1	31	from	effect	227:232	arg1	properties					254:263	physico-chemical properties	237:263	physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition	237:370	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	5	32	theme	fats	821:824	arg1	proportion					797:806	increased proportion	787:806	increased proportion of saturated fats	787:824	Increase in SFF with increased proportion of saturated fats in their composition, resulted in reduced wettability of powders.
32283375	2	33	theme	compositional	478:490	arg1	variation					492:500	compositional variation	478:500	compositional variation of IF	478:506	Results varied with compositional variation of IF.
32283375	5	34	theme	saturated	811:819	arg1	fats					821:824	saturated fats	811:824	saturated fats	811:824	Increase in SFF with increased proportion of saturated fats in their composition, resulted in reduced wettability of powders.
32283375	1	35	theme	lactose	133:139	arg1	LC					158:159	LC	158:159	LC	158:159	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	1	35	theme	lactose	133:139	arg1	crystallization					141:155	lactose crystallization	133:155	lactose crystallization (LC)	133:160	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	0	36	theme	free	63:66	arg1	fat					68:70	surface free fat	55:70	surface free fat	55:70	Inter-relationship between lactose crystallization and surface free fat during storage of infant formula.
32283375	4	37	theme	higher	666:671	arg1	proportion					673:682	higher proportion	666:682	higher proportion of unsaturated fats in SFF of powders with large lactose crystals and vice-versa	666:763	IF composition influenced LC and caused selective migration of triglycerides, resulting in higher proportion of unsaturated fats in SFF of powders with large lactose crystals and vice-versa.
32283375	1	38	theme	hydrolyzed	299:308	arg1	protein					343:349	hydrolyzed and intact (non-hydrolyzed) whey protein	299:349	protein	343:349	Inter-relationship between lactose crystallization (LC), the amount and composition of surface free fat (SFF); and their effect on physico-chemical properties of infant formula (IF) containing hydrolyzed and intact (non-hydrolyzed) whey protein in their composition were investigated at two temperatures (25 and 45 °C) and five RH (11-65%) conditions.
32283375	6	39	theme	migration	971:979	arg1	type					959:962	type	959:962	type	959:962	Overall, IF composition affects LC which influences the amount and type of fat migration to particle surface resulting in varying wettability of IF powders.
32283375	6	39	theme	migration	971:979	arg1	migration					971:979	fat migration	967:979	fat migration to particle surface resulting in varying wettability of IF powders	967:1046	Overall, IF composition affects LC which influences the amount and type of fat migration to particle surface resulting in varying wettability of IF powders.
32283375	6	39	theme	migration	971:979	arg1	amount					948:953	amount	948:953	amount	948:953	Overall, IF composition affects LC which influences the amount and type of fat migration to particle surface resulting in varying wettability of IF powders.
32283375	4	40	theme	unsaturated	687:697	arg1	fats					699:702	unsaturated fats	687:702	unsaturated fats in SFF of powders with large lactose crystals and vice-versa	687:763	IF composition influenced LC and caused selective migration of triglycerides, resulting in higher proportion of unsaturated fats in SFF of powders with large lactose crystals and vice-versa.
32283375	4	41	from	proportion	673:682	arg1	SFF					707:709	SFF	707:709	SFF of powders with large lactose crystals and vice-versa	707:763	IF composition influenced LC and caused selective migration of triglycerides, resulting in higher proportion of unsaturated fats in SFF of powders with large lactose crystals and vice-versa.
32283375	5	42	theme	powders	883:889	arg1	wettability					868:878	reduced wettability	860:878	reduced wettability of powders	860:889	Increase in SFF with increased proportion of saturated fats in their composition, resulted in reduced wettability of powders.
32283375	6	43	theme	particle	984:991	arg1	surface					993:999	particle surface	984:999	particle surface resulting in varying wettability of IF powders	984:1046	Overall, IF composition affects LC which influences the amount and type of fat migration to particle surface resulting in varying wettability of IF powders.
32283375	5	44	theme	increased	787:795	arg1	proportion					797:806	increased proportion	787:806	increased proportion of saturated fats	787:824	Increase in SFF with increased proportion of saturated fats in their composition, resulted in reduced wettability of powders.
32283375	6	45	theme	fat	967:969	arg1	migration					971:979	fat migration	967:979	fat migration to particle surface resulting in varying wettability of IF powders	967:1046	Overall, IF composition affects LC which influences the amount and type of fat migration to particle surface resulting in varying wettability of IF powders.
32283375	6	46	dep	amount	948:953	arg1	the					944:946	the	944:946	the	944:946	Overall, IF composition affects LC which influences the amount and type of fat migration to particle surface resulting in varying wettability of IF powders.
32283375	4	47	theme	triglycerides	638:650	arg1	migration					625:633	selective migration	615:633	selective migration of triglycerides	615:650	IF composition influenced LC and caused selective migration of triglycerides, resulting in higher proportion of unsaturated fats in SFF of powders with large lactose crystals and vice-versa.
32283375	4	48	from	fats	699:702	arg1	SFF					707:709	SFF	707:709	SFF of powders with large lactose crystals and vice-versa	707:763	IF composition influenced LC and caused selective migration of triglycerides, resulting in higher proportion of unsaturated fats in SFF of powders with large lactose crystals and vice-versa.
32870661	0	0	theme	Carbon	81:86	arg1	Yield					88:92	Carbon Yield	81:92	Carbon Yield	81:92	Close Packing of Cellulose and Chitosan in Regenerated Cellulose Fibers Improves Carbon Yield and Structural Properties of Respective Carbon Fibers.
32870661	4	1	theme	fiber	991:995	arg1	properties					967:976	mechanical properties	956:976	mechanical properties of cellulose fiber	956:995	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	1	2	theme	low	151:153	arg1	yield					162:166	A low carbon yield	149:166	A low carbon yield	149:166	A low carbon yield is a major limitation for the use of cellulose-based filaments as carbon fiber precursors.
32870661	1	2	theme	low	151:153	arg1	limitation					179:188	a major limitation	171:188	a major limitation for the use of cellulose-based filaments as carbon fiber precursors	171:256	A low carbon yield is a major limitation for the use of cellulose-based filaments as carbon fiber precursors.
32870661	1	3	theme	carbon	234:239	arg1	precursors					247:256	carbon fiber precursors	234:256	carbon fiber precursors	234:256	A low carbon yield is a major limitation for the use of cellulose-based filaments as carbon fiber precursors.
32870661	4	4	theme	cellulose	981:989	arg1	fiber					991:995	cellulose fiber	981:995	cellulose fiber	981:995	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	0	5	from	Packing	6:12	arg1	Fibers					65:70	Regenerated Cellulose Fibers	43:70	Regenerated Cellulose Fibers	43:70	Close Packing of Cellulose and Chitosan in Regenerated Cellulose Fibers Improves Carbon Yield and Structural Properties of Respective Carbon Fibers.
32870661	4	6	theme	pyrolysis	860:868	arg1	reaction					870:877	the pyrolysis reaction	856:877	the pyrolysis reaction	856:877	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	1	7	theme	carbon	155:160	arg1	yield					162:166	A low carbon yield	149:166	A low carbon yield	149:166	A low carbon yield is a major limitation for the use of cellulose-based filaments as carbon fiber precursors.
32870661	1	7	theme	carbon	155:160	arg1	limitation					179:188	a major limitation	171:188	a major limitation for the use of cellulose-based filaments as carbon fiber precursors	171:256	A low carbon yield is a major limitation for the use of cellulose-based filaments as carbon fiber precursors.
32870661	1	8	theme	fiber	241:245	arg1	precursors					247:256	carbon fiber precursors	234:256	carbon fiber precursors	234:256	A low carbon yield is a major limitation for the use of cellulose-based filaments as carbon fiber precursors.
32870661	4	9	theme	preservation	940:951	arg1	increase					907:914	a substantial increase	893:914	a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan	893:1052	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	4	10	theme	scattering	768:777	arg1	experiments					779:789	X-ray scattering experiments	762:789	X-ray scattering experiments	762:789	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	3	11	theme	direct	535:540	arg1	dissolution					542:552	direct dissolution	535:552	direct dissolution of cellulose and chitosan	535:578	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) was used for direct dissolution of cellulose and chitosan and to spin cellulose-chitosan composite fibers through a dry-jet wet spinning process (Ioncell).
32870661	4	12	theme	chitosan	741:748	arg1	distribution					693:704	homogenous distribution	682:704	homogenous distribution	682:704	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	4	12	theme	chitosan	741:748	arg1	packing					716:722	tight packing	710:722	tight packing	710:722	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	3	13	dep	used	526:529	arg1	Ioncell					668:674	Ioncell	668:674	Ioncell	668:674	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) was used for direct dissolution of cellulose and chitosan and to spin cellulose-chitosan composite fibers through a dry-jet wet spinning process (Ioncell).
32870661	4	14	theme	mechanical	956:965	arg1	properties					967:976	mechanical properties	956:976	mechanical properties of cellulose fiber	956:995	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	2	15	link	cellulose-derived	418:434	arg1	fiber					443:447	the cellulose-derived carbon fiber	414:447	the cellulose-derived carbon fiber	414:447	The present study aims to investigate the use of an abundant biopolymer chitosan as a natural charring agent particularly on enhancing the carbon yield of the cellulose-derived carbon fiber.
32870661	2	16	theme	present	263:269	arg1	study					271:275	The present study	259:275	The present study	259:275	The present study aims to investigate the use of an abundant biopolymer chitosan as a natural charring agent particularly on enhancing the carbon yield of the cellulose-derived carbon fiber.
32870661	0	17	theme	Structural	98:107	arg1	Properties					109:118	Structural Properties	98:118	Structural Properties	98:118	Close Packing of Cellulose and Chitosan in Regenerated Cellulose Fibers Improves Carbon Yield and Structural Properties of Respective Carbon Fibers.
32870661	2	18	theme	cellulose-derived	418:434	arg1	fiber					443:447	the cellulose-derived carbon fiber	414:447	the cellulose-derived carbon fiber	414:447	The present study aims to investigate the use of an abundant biopolymer chitosan as a natural charring agent particularly on enhancing the carbon yield of the cellulose-derived carbon fiber.
32870661	4	19	theme	yield	930:934	arg1	increase					907:914	a substantial increase	893:914	a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan	893:1052	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	2	20	theme	carbon	436:441	arg1	fiber					443:447	the cellulose-derived carbon fiber	414:447	the cellulose-derived carbon fiber	414:447	The present study aims to investigate the use of an abundant biopolymer chitosan as a natural charring agent particularly on enhancing the carbon yield of the cellulose-derived carbon fiber.
32870661	3	21	used	used	526:529	arg2	OAc					517:519	[DBNH]OAc	511:519	[DBNH]OAc	511:519	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) was used for direct dissolution of cellulose and chitosan and to spin cellulose-chitosan composite fibers through a dry-jet wet spinning process (Ioncell).
32870661	3	21	used	used	526:529	arg2	acetate					502:508	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate	450:508	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc)	450:520	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) was used for direct dissolution of cellulose and chitosan and to spin cellulose-chitosan composite fibers through a dry-jet wet spinning process (Ioncell).
32870661	0	22	theme	Close	0:4	arg1	Packing					6:12	Close Packing	0:12	Close Packing of Cellulose and Chitosan in Regenerated Cellulose Fibers	0:70	Close Packing of Cellulose and Chitosan in Regenerated Cellulose Fibers Improves Carbon Yield and Structural Properties of Respective Carbon Fibers.
32870661	2	23	theme	charring	353:360	arg1	agent					362:366	a natural charring agent	343:366	a natural charring agent	343:366	The present study aims to investigate the use of an abundant biopolymer chitosan as a natural charring agent particularly on enhancing the carbon yield of the cellulose-derived carbon fiber.
32870661	0	24	theme	Cellulose	17:25	arg1	Packing					6:12	Close Packing	0:12	Close Packing of Cellulose and Chitosan in Regenerated Cellulose Fibers	0:70	Close Packing of Cellulose and Chitosan in Regenerated Cellulose Fibers Improves Carbon Yield and Structural Properties of Respective Carbon Fibers.
32870661	0	25	theme	Respective	123:132	arg1	Fibers					141:146	Respective Carbon Fibers	123:146	Respective Carbon Fibers	123:146	Close Packing of Cellulose and Chitosan in Regenerated Cellulose Fibers Improves Carbon Yield and Structural Properties of Respective Carbon Fibers.
32870661	4	26	dep	distribution	693:704	arg1	The					678:680	The	678:680	The	678:680	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	4	27	theme	X-ray	762:766	arg1	experiments					779:789	X-ray scattering experiments	762:789	X-ray scattering experiments	762:789	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	4	28	theme	carbon	923:928	arg1	yield					930:934	carbon yield	923:934	carbon yield	923:934	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	2	29	theme	chitosan	331:338	arg1	use					301:303	the use	297:303	the use of an abundant biopolymer chitosan as a natural charring agent	297:366	The present study aims to investigate the use of an abundant biopolymer chitosan as a natural charring agent particularly on enhancing the carbon yield of the cellulose-derived carbon fiber.
32870661	2	30	theme	carbon	398:403	arg1	yield					405:409	the carbon yield	394:409	the carbon yield of the cellulose-derived carbon fiber	394:447	The present study aims to investigate the use of an abundant biopolymer chitosan as a natural charring agent particularly on enhancing the carbon yield of the cellulose-derived carbon fiber.
32870661	0	31	theme	Chitosan	31:38	arg1	Packing					6:12	Close Packing	0:12	Close Packing of Cellulose and Chitosan in Regenerated Cellulose Fibers	0:70	Close Packing of Cellulose and Chitosan in Regenerated Cellulose Fibers Improves Carbon Yield and Structural Properties of Respective Carbon Fibers.
32870661	3	32	theme	non-5-enium	490:500	arg1	OAc					517:519	[DBNH]OAc	511:519	[DBNH]OAc	511:519	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) was used for direct dissolution of cellulose and chitosan and to spin cellulose-chitosan composite fibers through a dry-jet wet spinning process (Ioncell).
32870661	3	32	theme	non-5-enium	490:500	arg1	acetate					502:508	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate	450:508	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc)	450:520	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) was used for direct dissolution of cellulose and chitosan and to spin cellulose-chitosan composite fibers through a dry-jet wet spinning process (Ioncell).
32870661	0	33	theme	Fibers	141:146	arg1	Yield					88:92	Carbon Yield	81:92	Carbon Yield	81:92	Close Packing of Cellulose and Chitosan in Regenerated Cellulose Fibers Improves Carbon Yield and Structural Properties of Respective Carbon Fibers.
32870661	0	33	theme	Fibers	141:146	arg1	Properties					109:118	Structural Properties	98:118	Structural Properties	98:118	Close Packing of Cellulose and Chitosan in Regenerated Cellulose Fibers Improves Carbon Yield and Structural Properties of Respective Carbon Fibers.
32870661	3	34	theme	dry-jet	638:644	arg1	process					659:665	a dry-jet wet spinning process	636:665	a dry-jet wet spinning process	636:665	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) was used for direct dissolution of cellulose and chitosan and to spin cellulose-chitosan composite fibers through a dry-jet wet spinning process (Ioncell).
32870661	3	35	theme	chitosan	571:578	arg1	dissolution					542:552	direct dissolution	535:552	direct dissolution of cellulose and chitosan	535:578	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) was used for direct dissolution of cellulose and chitosan and to spin cellulose-chitosan composite fibers through a dry-jet wet spinning process (Ioncell).
32870661	1	36	theme	major	173:177	arg1	yield					162:166	A low carbon yield	149:166	A low carbon yield	149:166	A low carbon yield is a major limitation for the use of cellulose-based filaments as carbon fiber precursors.
32870661	1	36	theme	major	173:177	arg1	limitation					179:188	a major limitation	171:188	a major limitation for the use of cellulose-based filaments as carbon fiber precursors	171:256	A low carbon yield is a major limitation for the use of cellulose-based filaments as carbon fiber precursors.
32870661	4	37	theme	other	1009:1013	arg1	xylan					1048:1052	xylan	1048:1052	xylan	1048:1052	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	4	37	theme	other	1009:1013	arg1	cobiopolymers					1015:1027	other cobiopolymers	1009:1027	other cobiopolymers such as lignin and xylan	1009:1052	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	4	37	theme	other	1009:1013	arg1	lignin					1037:1042	lignin	1037:1042	lignin	1037:1042	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	0	38	theme	Carbon	134:139	arg1	Fibers					141:146	Respective Carbon Fibers	123:146	Respective Carbon Fibers	123:146	Close Packing of Cellulose and Chitosan in Regenerated Cellulose Fibers Improves Carbon Yield and Structural Properties of Respective Carbon Fibers.
32870661	4	39	theme	synergistic	800:810	arg1	interaction					812:822	a synergistic interaction	798:822	a synergistic interaction between the two polymers	798:847	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	0	40	theme	Regenerated	43:53	arg1	Fibers					65:70	Regenerated Cellulose Fibers	43:70	Regenerated Cellulose Fibers	43:70	Close Packing of Cellulose and Chitosan in Regenerated Cellulose Fibers Improves Carbon Yield and Structural Properties of Respective Carbon Fibers.
32870661	4	41	dep	yield	930:934	arg1	the					919:921	the	919:921	the	919:921	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	4	42	theme	properties	967:976	arg1	preservation					940:951	preservation	940:951	preservation	940:951	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	4	42	theme	properties	967:976	arg1	yield					930:934	carbon yield	923:934	carbon yield	923:934	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	3	43	theme	cellulose	557:565	arg1	dissolution					542:552	direct dissolution	535:552	direct dissolution of cellulose and chitosan	535:578	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) was used for direct dissolution of cellulose and chitosan and to spin cellulose-chitosan composite fibers through a dry-jet wet spinning process (Ioncell).
32870661	2	44	theme	biopolymer	320:329	arg1	chitosan					331:338	an abundant biopolymer chitosan	308:338	an abundant biopolymer chitosan	308:338	The present study aims to investigate the use of an abundant biopolymer chitosan as a natural charring agent particularly on enhancing the carbon yield of the cellulose-derived carbon fiber.
32870661	2	45	theme	fiber	443:447	arg1	yield					405:409	the carbon yield	394:409	the carbon yield of the cellulose-derived carbon fiber	394:447	The present study aims to investigate the use of an abundant biopolymer chitosan as a natural charring agent particularly on enhancing the carbon yield of the cellulose-derived carbon fiber.
32870661	2	46	theme	abundant	311:318	arg1	chitosan					331:338	an abundant biopolymer chitosan	308:338	an abundant biopolymer chitosan	308:338	The present study aims to investigate the use of an abundant biopolymer chitosan as a natural charring agent particularly on enhancing the carbon yield of the cellulose-derived carbon fiber.
32870661	4	47	theme	tight	710:714	arg1	packing					716:722	tight packing	710:722	tight packing	710:722	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	3	48	theme	cellulose-chitosan	592:609	arg1	fibers					621:626	cellulose-chitosan composite fibers	592:626	cellulose-chitosan composite fibers	592:626	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) was used for direct dissolution of cellulose and chitosan and to spin cellulose-chitosan composite fibers through a dry-jet wet spinning process (Ioncell).
32870661	3	49	theme	liquid	460:465	arg1	OAc					517:519	[DBNH]OAc	511:519	[DBNH]OAc	511:519	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) was used for direct dissolution of cellulose and chitosan and to spin cellulose-chitosan composite fibers through a dry-jet wet spinning process (Ioncell).
32870661	3	49	theme	liquid	460:465	arg1	acetate					502:508	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate	450:508	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc)	450:520	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) was used for direct dissolution of cellulose and chitosan and to spin cellulose-chitosan composite fibers through a dry-jet wet spinning process (Ioncell).
32870661	4	50	theme	substantial	895:905	arg1	increase					907:914	a substantial increase	893:914	a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan	893:1052	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	3	51	theme	wet	646:648	arg1	process					659:665	a dry-jet wet spinning process	636:665	a dry-jet wet spinning process	636:665	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) was used for direct dissolution of cellulose and chitosan and to spin cellulose-chitosan composite fibers through a dry-jet wet spinning process (Ioncell).
32870661	3	52	theme	composite	611:619	arg1	fibers					621:626	cellulose-chitosan composite fibers	592:626	cellulose-chitosan composite fibers	592:626	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) was used for direct dissolution of cellulose and chitosan and to spin cellulose-chitosan composite fibers through a dry-jet wet spinning process (Ioncell).
32870661	4	53	theme	cellulose	727:735	arg1	distribution					693:704	homogenous distribution	682:704	homogenous distribution	682:704	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	4	53	theme	cellulose	727:735	arg1	packing					716:722	tight packing	710:722	tight packing	710:722	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
32870661	3	54	theme	1,5-diazabicyclo[4.3.0	467:488	arg1	OAc					517:519	[DBNH]OAc	511:519	[DBNH]OAc	511:519	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) was used for direct dissolution of cellulose and chitosan and to spin cellulose-chitosan composite fibers through a dry-jet wet spinning process (Ioncell).
32870661	3	54	theme	1,5-diazabicyclo[4.3.0	467:488	arg1	acetate					502:508	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate	450:508	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc)	450:520	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) was used for direct dissolution of cellulose and chitosan and to spin cellulose-chitosan composite fibers through a dry-jet wet spinning process (Ioncell).
32870661	3	55	theme	ionic	454:458	arg1	OAc					517:519	[DBNH]OAc	511:519	[DBNH]OAc	511:519	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) was used for direct dissolution of cellulose and chitosan and to spin cellulose-chitosan composite fibers through a dry-jet wet spinning process (Ioncell).
32870661	3	55	theme	ionic	454:458	arg1	acetate					502:508	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate	450:508	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc)	450:520	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) was used for direct dissolution of cellulose and chitosan and to spin cellulose-chitosan composite fibers through a dry-jet wet spinning process (Ioncell).
32870661	1	56	theme	cellulose-based	205:219	arg1	filaments					221:229	cellulose-based filaments	205:229	cellulose-based filaments	205:229	A low carbon yield is a major limitation for the use of cellulose-based filaments as carbon fiber precursors.
32870661	3	57	theme	spinning	650:657	arg1	process					659:665	a dry-jet wet spinning process	636:665	a dry-jet wet spinning process	636:665	The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) was used for direct dissolution of cellulose and chitosan and to spin cellulose-chitosan composite fibers through a dry-jet wet spinning process (Ioncell).
32870661	2	58	theme	natural	345:351	arg1	agent					362:366	a natural charring agent	343:366	a natural charring agent	343:366	The present study aims to investigate the use of an abundant biopolymer chitosan as a natural charring agent particularly on enhancing the carbon yield of the cellulose-derived carbon fiber.
32870661	1	59	theme	filaments	221:229	arg1	use					198:200	the use	194:200	the use of cellulose-based filaments as carbon fiber precursors	194:256	A low carbon yield is a major limitation for the use of cellulose-based filaments as carbon fiber precursors.
32870661	0	60	theme	Cellulose	55:63	arg1	Fibers					65:70	Regenerated Cellulose Fibers	43:70	Regenerated Cellulose Fibers	43:70	Close Packing of Cellulose and Chitosan in Regenerated Cellulose Fibers Improves Carbon Yield and Structural Properties of Respective Carbon Fibers.
32870661	4	61	theme	homogenous	682:691	arg1	distribution					693:704	homogenous distribution	682:704	homogenous distribution	682:704	The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.
34971644	8	0	theme	thermal	1188:1194	arg1	stability					1196:1204	the thermal stability	1184:1204	the thermal stability of the AG fiber	1184:1220	The crystal index affected the thermal stability of the AG fiber.
34971644	6	1	theme	raw	996:998	arg1	fiber					1003:1007	raw AG fiber	996:1007	raw AG fiber	996:1007	The bleaching treatment increased the crystal index by 48.3% compared to raw AG fiber, along with an increase in the cellulose content of 20.4%.
34971644	5	2	theme	grinding	717:724	arg1	chemical-ultrafine					698:715	chemical-ultrafine grinding	698:724	chemical-ultrafine grinding	698:724	The effect of chemical-ultrafine grinding on the morphology and properties of AG fiber was identified using chemical composition, Scanning Electron Microscopy, X-ray Diffraction, Fourier Transform Infrared, and Thermogravimetric Analysis.
34971644	1	3	theme	much	181:184	arg1	interest					186:193	much interest	181:193	much interest	181:193	Nanocellulose is a renewable and biocompatible nanomaterial that evokes much interest because of its versatility in various applications.
34971644	4	4	theme	average	637:643	arg1	diameter					645:652	the average diameter	633:652	the average diameter of nanocellulose	633:669	From the observation of Transmission Electron Microscopy, the average diameter of nanocellulose was 4.07 nm.
34971644	4	4	theme	average	637:643	arg1	4.07 nm					675:681	4.07 nm	675:681	4.07 nm	675:681	From the observation of Transmission Electron Microscopy, the average diameter of nanocellulose was 4.07 nm.
34971644	0	5	theme	chemical-mechanical	78:96	arg1	treatment					98:106	chemical-mechanical treatment	78:106	chemical-mechanical treatment	78:106	Isolation and characterization of cellulose nanofibers from Agave gigantea by chemical-mechanical treatment.
34971644	12	6	theme	polymer	1708:1714	arg1	biocomposites					1716:1728	environmentally friendly polymer biocomposites	1683:1728	environmentally friendly polymer biocomposites	1683:1728	As a result, nanocellulose derived from AG fiber can be applied as reinforcement in environmentally friendly polymer biocomposites.
34971644	5	7	theme	chemical	792:799	arg1	composition					801:811	chemical composition	792:811	chemical composition	792:811	The effect of chemical-ultrafine grinding on the morphology and properties of AG fiber was identified using chemical composition, Scanning Electron Microscopy, X-ray Diffraction, Fourier Transform Infrared, and Thermogravimetric Analysis.
34971644	9	8	theme	AG	1332:1333	arg1	fiber					1335:1339	AG fiber	1332:1339	AG fiber	1332:1339	The TGA results showed that AG fiber treated with bleaching showed the highest thermal stability compared to AG fiber without treatment.
34971644	7	9	theme	fiber	1150:1154	arg1	content					1132:1138	the crystal content	1120:1138	the crystal content of the AG fiber	1120:1154	The ultrafine grinding process caused a decrease in the crystal content of the AG fiber.
34971644	11	10	theme	hemicellulose	1554:1566	arg1	groups					1579:1584	lignin and hemicellulose functional groups	1543:1584	lignin and hemicellulose functional groups	1543:1584	After chemical treatment, the peaks at 1605 and 1243 cm-1 disappeared, indicating the loss of lignin and hemicellulose functional groups in AG fiber.
34971644	2	11	theme	AG	319:320	arg1	fiber					323:327	Agave gigantea (AG) fiber	303:327	Agave gigantea (AG) fiber using the chemical-ultrafine grinding treatment	303:375	This study reports the production of nanocellulose from Agave gigantea (AG) fiber using the chemical-ultrafine grinding treatment.
34971644	12	12	theme	friendly	1699:1706	arg1	biocomposites					1716:1728	environmentally friendly polymer biocomposites	1683:1728	environmentally friendly polymer biocomposites	1683:1728	As a result, nanocellulose derived from AG fiber can be applied as reinforcement in environmentally friendly polymer biocomposites.
34971644	9	13	dep	showed	1283:1288	arg1	compared					1320:1327	compared	1320:1327	showed the highest thermal stability compared to AG fiber without treatment	1283:1357	The TGA results showed that AG fiber treated with bleaching showed the highest thermal stability compared to AG fiber without treatment.
34971644	10	14	theme	vibrations	1409:1418	arg1	presence					1394:1401	the presence	1390:1401	the presence of CH vibrations from the ether in the fiber	1390:1446	The FTIR analysis showed that the presence of CH vibrations from the ether in the fiber.
34971644	3	15	theme	microfibrils	542:553	arg1	size					524:527	the size	520:527	the size of cellulose microfibrils into nanocellulose	520:572	Chemical treatment (alkalization and bleaching) removed non-cellulose components (hemicellulose and lignin), while ultrafine grinding reduced the size of cellulose microfibrils into nanocellulose.
34971644	11	16	theme	chemical	1455:1462	arg1	treatment					1464:1472	chemical treatment	1455:1472	chemical treatment	1455:1472	After chemical treatment, the peaks at 1605 and 1243 cm-1 disappeared, indicating the loss of lignin and hemicellulose functional groups in AG fiber.
34971644	6	17	theme	cellulose	1040:1048	arg1	content					1050:1056	the cellulose content	1036:1056	the cellulose content of 20.4%	1036:1065	The bleaching treatment increased the crystal index by 48.3% compared to raw AG fiber, along with an increase in the cellulose content of 20.4%.
34971644	3	18	theme	Chemical	378:385	arg1	treatment					387:395	Chemical treatment	378:395	Chemical treatment (alkalization and bleaching)	378:424	Chemical treatment (alkalization and bleaching) removed non-cellulose components (hemicellulose and lignin), while ultrafine grinding reduced the size of cellulose microfibrils into nanocellulose.
34971644	5	19	dep	Fourier	863:869	arg1	Transform					871:879	Transform	871:879	Transform Infrared	871:888	The effect of chemical-ultrafine grinding on the morphology and properties of AG fiber was identified using chemical composition, Scanning Electron Microscopy, X-ray Diffraction, Fourier Transform Infrared, and Thermogravimetric Analysis.
34971644	9	20	theme	TGA	1227:1229	arg1	results					1231:1237	The TGA results	1223:1237	The TGA results	1223:1237	The TGA results showed that AG fiber treated with bleaching showed the highest thermal stability compared to AG fiber without treatment.
34971644	2	21	theme	gigantea	309:316	arg1	fiber					323:327	Agave gigantea (AG) fiber	303:327	Agave gigantea (AG) fiber using the chemical-ultrafine grinding treatment	303:375	This study reports the production of nanocellulose from Agave gigantea (AG) fiber using the chemical-ultrafine grinding treatment.
34971644	5	22	theme	Scanning	814:821	arg1	Microscopy					832:841	Scanning Electron Microscopy	814:841	Scanning Electron Microscopy	814:841	The effect of chemical-ultrafine grinding on the morphology and properties of AG fiber was identified using chemical composition, Scanning Electron Microscopy, X-ray Diffraction, Fourier Transform Infrared, and Thermogravimetric Analysis.
34971644	3	23	dep	components	448:457	arg1	components					448:457	non-cellulose components	434:457	non-cellulose components (hemicellulose and lignin)	434:484	Chemical treatment (alkalization and bleaching) removed non-cellulose components (hemicellulose and lignin), while ultrafine grinding reduced the size of cellulose microfibrils into nanocellulose.
34971644	3	23	dep	components	448:457	arg1	lignin					478:483	lignin	478:483	lignin	478:483	Chemical treatment (alkalization and bleaching) removed non-cellulose components (hemicellulose and lignin), while ultrafine grinding reduced the size of cellulose microfibrils into nanocellulose.
34971644	3	23	dep	components	448:457	arg1	hemicellulose					460:472	hemicellulose	460:472	hemicellulose	460:472	Chemical treatment (alkalization and bleaching) removed non-cellulose components (hemicellulose and lignin), while ultrafine grinding reduced the size of cellulose microfibrils into nanocellulose.
34971644	2	24	theme	Agave	303:307	arg1	fiber					323:327	Agave gigantea (AG) fiber	303:327	Agave gigantea (AG) fiber using the chemical-ultrafine grinding treatment	303:375	This study reports the production of nanocellulose from Agave gigantea (AG) fiber using the chemical-ultrafine grinding treatment.
34971644	8	25	theme	AG	1213:1214	arg1	fiber					1216:1220	the AG fiber	1209:1220	the AG fiber	1209:1220	The crystal index affected the thermal stability of the AG fiber.
34971644	8	26	theme	fiber	1216:1220	arg1	stability					1196:1204	the thermal stability	1184:1204	the thermal stability of the AG fiber	1184:1220	The crystal index affected the thermal stability of the AG fiber.
34971644	5	27	theme	X-ray	844:848	arg1	Diffraction					850:860	X-ray Diffraction	844:860	X-ray Diffraction	844:860	The effect of chemical-ultrafine grinding on the morphology and properties of AG fiber was identified using chemical composition, Scanning Electron Microscopy, X-ray Diffraction, Fourier Transform Infrared, and Thermogravimetric Analysis.
34971644	10	28	attach	presence	1394:1401	arg2	vibrations					1409:1418	CH vibrations	1406:1418	CH vibrations from the ether	1406:1433	The FTIR analysis showed that the presence of CH vibrations from the ether in the fiber.
34971644	10	28	attach	presence	1394:1401	arg1	fiber					1442:1446	the fiber	1438:1446	the fiber	1438:1446	The FTIR analysis showed that the presence of CH vibrations from the ether in the fiber.
34971644	11	29	from	1605	1488:1491	arg1	peaks					1479:1483	the peaks	1475:1483	the peaks at 1605 and 1243 cm-1	1475:1505	After chemical treatment, the peaks at 1605 and 1243 cm-1 disappeared, indicating the loss of lignin and hemicellulose functional groups in AG fiber.
34971644	7	30	from	decrease	1108:1115	arg1	content					1132:1138	the crystal content	1120:1138	the crystal content of the AG fiber	1120:1154	The ultrafine grinding process caused a decrease in the crystal content of the AG fiber.
34971644	5	31	dep	morphology	733:742	arg1	the					729:731	the	729:731	the	729:731	The effect of chemical-ultrafine grinding on the morphology and properties of AG fiber was identified using chemical composition, Scanning Electron Microscopy, X-ray Diffraction, Fourier Transform Infrared, and Thermogravimetric Analysis.
34971644	4	32	theme	Electron	612:619	arg1	Microscopy					621:630	Transmission Electron Microscopy	599:630	Transmission Electron Microscopy	599:630	From the observation of Transmission Electron Microscopy, the average diameter of nanocellulose was 4.07 nm.
34971644	5	33	theme	chemical-ultrafine	698:715	arg1	effect					688:693	The effect	684:693	The effect of chemical-ultrafine grinding on the morphology and properties of AG fiber	684:769	The effect of chemical-ultrafine grinding on the morphology and properties of AG fiber was identified using chemical composition, Scanning Electron Microscopy, X-ray Diffraction, Fourier Transform Infrared, and Thermogravimetric Analysis.
34971644	11	34	theme	functional	1568:1577	arg1	groups					1579:1584	lignin and hemicellulose functional groups	1543:1584	lignin and hemicellulose functional groups	1543:1584	After chemical treatment, the peaks at 1605 and 1243 cm-1 disappeared, indicating the loss of lignin and hemicellulose functional groups in AG fiber.
34971644	11	35	theme	AG	1589:1590	arg1	fiber					1592:1596	AG fiber	1589:1596	AG fiber	1589:1596	After chemical treatment, the peaks at 1605 and 1243 cm-1 disappeared, indicating the loss of lignin and hemicellulose functional groups in AG fiber.
34971644	12	36	theme	AG	1639:1640	arg1	fiber					1642:1646	AG fiber	1639:1646	AG fiber	1639:1646	As a result, nanocellulose derived from AG fiber can be applied as reinforcement in environmentally friendly polymer biocomposites.
34971644	4	37	theme	Transmission	599:610	arg1	Microscopy					621:630	Transmission Electron Microscopy	599:630	Transmission Electron Microscopy	599:630	From the observation of Transmission Electron Microscopy, the average diameter of nanocellulose was 4.07 nm.
34971644	5	38	theme	fiber	765:769	arg1	properties					748:757	properties	748:757	properties	748:757	The effect of chemical-ultrafine grinding on the morphology and properties of AG fiber was identified using chemical composition, Scanning Electron Microscopy, X-ray Diffraction, Fourier Transform Infrared, and Thermogravimetric Analysis.
34971644	5	38	theme	fiber	765:769	arg1	morphology					733:742	morphology	733:742	morphology	733:742	The effect of chemical-ultrafine grinding on the morphology and properties of AG fiber was identified using chemical composition, Scanning Electron Microscopy, X-ray Diffraction, Fourier Transform Infrared, and Thermogravimetric Analysis.
34971644	9	39	theme	AG	1251:1252	arg1	fiber					1254:1258	AG fiber	1251:1258	AG fiber treated with bleaching	1251:1281	The TGA results showed that AG fiber treated with bleaching showed the highest thermal stability compared to AG fiber without treatment.
34971644	11	40	from	1243 cm-1	1497:1505	arg1	peaks					1479:1483	the peaks	1475:1483	the peaks at 1605 and 1243 cm-1	1475:1505	After chemical treatment, the peaks at 1605 and 1243 cm-1 disappeared, indicating the loss of lignin and hemicellulose functional groups in AG fiber.
34971644	7	41	theme	AG	1147:1148	arg1	fiber					1150:1154	the AG fiber	1143:1154	the AG fiber	1143:1154	The ultrafine grinding process caused a decrease in the crystal content of the AG fiber.
34971644	3	42	theme	grinding	503:510	arg1	ultrafine					493:501	ultrafine grinding	493:510	ultrafine grinding	493:510	Chemical treatment (alkalization and bleaching) removed non-cellulose components (hemicellulose and lignin), while ultrafine grinding reduced the size of cellulose microfibrils into nanocellulose.
34971644	5	43	theme	Thermogravimetric	895:911	arg1	Analysis					913:920	Thermogravimetric Analysis	895:920	Thermogravimetric Analysis	895:920	The effect of chemical-ultrafine grinding on the morphology and properties of AG fiber was identified using chemical composition, Scanning Electron Microscopy, X-ray Diffraction, Fourier Transform Infrared, and Thermogravimetric Analysis.
34971644	7	44	theme	crystal	1124:1130	arg1	content					1132:1138	the crystal content	1120:1138	the crystal content of the AG fiber	1120:1154	The ultrafine grinding process caused a decrease in the crystal content of the AG fiber.
34971644	6	45	theme	crystal	961:967	arg1	index					969:973	the crystal index	957:973	the crystal index	957:973	The bleaching treatment increased the crystal index by 48.3% compared to raw AG fiber, along with an increase in the cellulose content of 20.4%.
34971644	10	46	dep	showed	1378:1383	arg1	presence					1394:1401	the presence	1390:1401	the presence of CH vibrations from the ether in the fiber	1390:1446	The FTIR analysis showed that the presence of CH vibrations from the ether in the fiber.
34971644	1	47	theme	various	225:231	arg1	applications					233:244	various applications	225:244	various applications	225:244	Nanocellulose is a renewable and biocompatible nanomaterial that evokes much interest because of its versatility in various applications.
34971644	4	48	theme	Microscopy	621:630	arg1	observation					584:594	the observation	580:594	the observation of Transmission Electron Microscopy	580:630	From the observation of Transmission Electron Microscopy, the average diameter of nanocellulose was 4.07 nm.
34971644	12	49	theme	environmentally	1683:1697	arg1	biocomposites					1716:1728	environmentally friendly polymer biocomposites	1683:1728	environmentally friendly polymer biocomposites	1683:1728	As a result, nanocellulose derived from AG fiber can be applied as reinforcement in environmentally friendly polymer biocomposites.
34971644	0	50	theme	cellulose	34:42	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Isolation and characterization of cellulose nanofibers from Agave gigantea by chemical-mechanical treatment.
34971644	0	50	theme	cellulose	34:42	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and characterization of cellulose nanofibers from Agave gigantea by chemical-mechanical treatment.
34971644	4	51	from	observation	584:594	arg1	diameter					645:652	the average diameter	633:652	the average diameter of nanocellulose	633:669	From the observation of Transmission Electron Microscopy, the average diameter of nanocellulose was 4.07 nm.
34971644	4	51	from	observation	584:594	arg1	4.07 nm					675:681	4.07 nm	675:681	4.07 nm	675:681	From the observation of Transmission Electron Microscopy, the average diameter of nanocellulose was 4.07 nm.
34971644	11	52	from	loss	1535:1538	arg1	fiber					1592:1596	AG fiber	1589:1596	AG fiber	1589:1596	After chemical treatment, the peaks at 1605 and 1243 cm-1 disappeared, indicating the loss of lignin and hemicellulose functional groups in AG fiber.
34971644	10	53	from	presence	1394:1401	arg1	ether					1429:1433	the ether	1425:1433	the ether	1425:1433	The FTIR analysis showed that the presence of CH vibrations from the ether in the fiber.
34971644	10	53	from	presence	1394:1401	arg1	fiber					1442:1446	the fiber	1438:1446	the fiber	1438:1446	The FTIR analysis showed that the presence of CH vibrations from the ether in the fiber.
34971644	3	54	theme	non-cellulose	434:446	arg1	components					448:457	non-cellulose components	434:457	non-cellulose components (hemicellulose and lignin)	434:484	Chemical treatment (alkalization and bleaching) removed non-cellulose components (hemicellulose and lignin), while ultrafine grinding reduced the size of cellulose microfibrils into nanocellulose.
34971644	3	54	theme	non-cellulose	434:446	arg1	lignin					478:483	lignin	478:483	lignin	478:483	Chemical treatment (alkalization and bleaching) removed non-cellulose components (hemicellulose and lignin), while ultrafine grinding reduced the size of cellulose microfibrils into nanocellulose.
34971644	3	54	theme	non-cellulose	434:446	arg1	hemicellulose					460:472	hemicellulose	460:472	hemicellulose	460:472	Chemical treatment (alkalization and bleaching) removed non-cellulose components (hemicellulose and lignin), while ultrafine grinding reduced the size of cellulose microfibrils into nanocellulose.
34971644	7	55	theme	ultrafine	1072:1080	arg1	process					1091:1097	The ultrafine grinding process	1068:1097	The ultrafine grinding process	1068:1097	The ultrafine grinding process caused a decrease in the crystal content of the AG fiber.
34971644	1	56	theme	renewable	128:136	arg1	Nanocellulose					109:121	Nanocellulose	109:121	Nanocellulose	109:121	Nanocellulose is a renewable and biocompatible nanomaterial that evokes much interest because of its versatility in various applications.
34971644	1	56	theme	renewable	128:136	arg1	nanomaterial					156:167	a renewable and biocompatible nanomaterial	126:167	a renewable and biocompatible nanomaterial that evokes much interest because of its versatility in various applications	126:244	Nanocellulose is a renewable and biocompatible nanomaterial that evokes much interest because of its versatility in various applications.
34971644	4	57	theme	nanocellulose	657:669	arg1	diameter					645:652	the average diameter	633:652	the average diameter of nanocellulose	633:669	From the observation of Transmission Electron Microscopy, the average diameter of nanocellulose was 4.07 nm.
34971644	4	57	theme	nanocellulose	657:669	arg1	4.07 nm					675:681	4.07 nm	675:681	4.07 nm	675:681	From the observation of Transmission Electron Microscopy, the average diameter of nanocellulose was 4.07 nm.
34971644	3	58	dep	treatment	387:395	arg1	bleaching					415:423	bleaching	415:423	bleaching	415:423	Chemical treatment (alkalization and bleaching) removed non-cellulose components (hemicellulose and lignin), while ultrafine grinding reduced the size of cellulose microfibrils into nanocellulose.
34971644	3	58	dep	treatment	387:395	arg1	alkalization					398:409	alkalization	398:409	alkalization	398:409	Chemical treatment (alkalization and bleaching) removed non-cellulose components (hemicellulose and lignin), while ultrafine grinding reduced the size of cellulose microfibrils into nanocellulose.
34971644	6	59	theme	bleaching	927:935	arg1	treatment					937:945	The bleaching treatment	923:945	The bleaching treatment	923:945	The bleaching treatment increased the crystal index by 48.3% compared to raw AG fiber, along with an increase in the cellulose content of 20.4%.
34971644	5	60	dep	Transform	871:879	arg1	Infrared					881:888	Infrared	881:888	Transform Infrared	871:888	The effect of chemical-ultrafine grinding on the morphology and properties of AG fiber was identified using chemical composition, Scanning Electron Microscopy, X-ray Diffraction, Fourier Transform Infrared, and Thermogravimetric Analysis.
34971644	10	61	theme	FTIR	1364:1367	arg1	analysis					1369:1376	The FTIR analysis	1360:1376	The FTIR analysis	1360:1376	The FTIR analysis showed that the presence of CH vibrations from the ether in the fiber.
34971644	3	62	theme	cellulose	532:540	arg1	microfibrils					542:553	cellulose microfibrils	532:553	cellulose microfibrils into nanocellulose	532:572	Chemical treatment (alkalization and bleaching) removed non-cellulose components (hemicellulose and lignin), while ultrafine grinding reduced the size of cellulose microfibrils into nanocellulose.
34971644	11	63	theme	lignin	1543:1548	arg1	groups					1579:1584	lignin and hemicellulose functional groups	1543:1584	lignin and hemicellulose functional groups	1543:1584	After chemical treatment, the peaks at 1605 and 1243 cm-1 disappeared, indicating the loss of lignin and hemicellulose functional groups in AG fiber.
34971644	7	64	theme	grinding	1082:1089	arg1	process					1091:1097	The ultrafine grinding process	1068:1097	The ultrafine grinding process	1068:1097	The ultrafine grinding process caused a decrease in the crystal content of the AG fiber.
34971644	5	65	from	effect	688:693	arg1	properties					748:757	properties	748:757	properties	748:757	The effect of chemical-ultrafine grinding on the morphology and properties of AG fiber was identified using chemical composition, Scanning Electron Microscopy, X-ray Diffraction, Fourier Transform Infrared, and Thermogravimetric Analysis.
34971644	5	65	from	effect	688:693	arg1	morphology					733:742	morphology	733:742	morphology	733:742	The effect of chemical-ultrafine grinding on the morphology and properties of AG fiber was identified using chemical composition, Scanning Electron Microscopy, X-ray Diffraction, Fourier Transform Infrared, and Thermogravimetric Analysis.
34971644	12	66	attach	derived	1626:1632	arg1	fiber					1642:1646	AG fiber	1639:1646	AG fiber	1639:1646	As a result, nanocellulose derived from AG fiber can be applied as reinforcement in environmentally friendly polymer biocomposites.
34971644	12	66	attach	derived	1626:1632	arg2	reinforcement					1666:1678	reinforcement	1666:1678	reinforcement	1666:1678	As a result, nanocellulose derived from AG fiber can be applied as reinforcement in environmentally friendly polymer biocomposites.
34971644	12	66	attach	derived	1626:1632	arg2	nanocellulose					1612:1624	nanocellulose	1612:1624	nanocellulose derived from AG fiber	1612:1646	As a result, nanocellulose derived from AG fiber can be applied as reinforcement in environmentally friendly polymer biocomposites.
34971644	12	66	attach	derived	1626:1632	arg2	result					1604:1609	a result	1602:1609	a result	1602:1609	As a result, nanocellulose derived from AG fiber can be applied as reinforcement in environmentally friendly polymer biocomposites.
34971644	1	67	theme	biocompatible	142:154	arg1	Nanocellulose					109:121	Nanocellulose	109:121	Nanocellulose	109:121	Nanocellulose is a renewable and biocompatible nanomaterial that evokes much interest because of its versatility in various applications.
34971644	1	67	theme	biocompatible	142:154	arg1	nanomaterial					156:167	a renewable and biocompatible nanomaterial	126:167	a renewable and biocompatible nanomaterial that evokes much interest because of its versatility in various applications	126:244	Nanocellulose is a renewable and biocompatible nanomaterial that evokes much interest because of its versatility in various applications.
34971644	11	68	theme	groups	1579:1584	arg1	loss					1535:1538	the loss	1531:1538	the loss of lignin and hemicellulose functional groups in AG fiber	1531:1596	After chemical treatment, the peaks at 1605 and 1243 cm-1 disappeared, indicating the loss of lignin and hemicellulose functional groups in AG fiber.
34971644	5	69	theme	AG	762:763	arg1	fiber					765:769	AG fiber	762:769	AG fiber	762:769	The effect of chemical-ultrafine grinding on the morphology and properties of AG fiber was identified using chemical composition, Scanning Electron Microscopy, X-ray Diffraction, Fourier Transform Infrared, and Thermogravimetric Analysis.
34971644	9	70	theme	highest	1294:1300	arg1	stability					1310:1318	the highest thermal stability	1290:1318	the highest thermal stability	1290:1318	The TGA results showed that AG fiber treated with bleaching showed the highest thermal stability compared to AG fiber without treatment.
34971644	2	71	theme	nanocellulose	284:296	arg1	production					270:279	the production	266:279	the production of nanocellulose from Agave gigantea (AG) fiber using the chemical-ultrafine grinding treatment	266:375	This study reports the production of nanocellulose from Agave gigantea (AG) fiber using the chemical-ultrafine grinding treatment.
34971644	8	72	theme	crystal	1161:1167	arg1	index					1169:1173	The crystal index	1157:1173	The crystal index	1157:1173	The crystal index affected the thermal stability of the AG fiber.
34971644	2	73	theme	chemical-ultrafine	339:356	arg1	treatment					367:375	the chemical-ultrafine grinding treatment	335:375	the chemical-ultrafine grinding treatment	335:375	This study reports the production of nanocellulose from Agave gigantea (AG) fiber using the chemical-ultrafine grinding treatment.
34971644	2	74	theme	grinding	358:365	arg1	treatment					367:375	the chemical-ultrafine grinding treatment	335:375	the chemical-ultrafine grinding treatment	335:375	This study reports the production of nanocellulose from Agave gigantea (AG) fiber using the chemical-ultrafine grinding treatment.
34971644	5	75	theme	Electron	823:830	arg1	Microscopy					832:841	Scanning Electron Microscopy	814:841	Scanning Electron Microscopy	814:841	The effect of chemical-ultrafine grinding on the morphology and properties of AG fiber was identified using chemical composition, Scanning Electron Microscopy, X-ray Diffraction, Fourier Transform Infrared, and Thermogravimetric Analysis.
34971644	9	76	theme	thermal	1302:1308	arg1	stability					1310:1318	the highest thermal stability	1290:1318	the highest thermal stability	1290:1318	The TGA results showed that AG fiber treated with bleaching showed the highest thermal stability compared to AG fiber without treatment.
34971644	1	77	from	versatility	210:220	arg1	applications					233:244	various applications	225:244	various applications	225:244	Nanocellulose is a renewable and biocompatible nanomaterial that evokes much interest because of its versatility in various applications.
34971644	6	78	from	increase	1024:1031	arg1	content					1050:1056	the cellulose content	1036:1056	the cellulose content of 20.4%	1036:1065	The bleaching treatment increased the crystal index by 48.3% compared to raw AG fiber, along with an increase in the cellulose content of 20.4%.
34971644	10	79	theme	CH	1406:1407	arg1	vibrations					1409:1418	CH vibrations	1406:1418	CH vibrations from the ether	1406:1433	The FTIR analysis showed that the presence of CH vibrations from the ether in the fiber.
34971644	0	80	theme	Agave	60:64	arg1	gigantea					66:73	Agave gigantea	60:73	Agave gigantea	60:73	Isolation and characterization of cellulose nanofibers from Agave gigantea by chemical-mechanical treatment.
34971644	6	81	theme	AG	1000:1001	arg1	fiber					1003:1007	raw AG fiber	996:1007	raw AG fiber	996:1007	The bleaching treatment increased the crystal index by 48.3% compared to raw AG fiber, along with an increase in the cellulose content of 20.4%.
34971644	10	82	from	ether	1429:1433	arg1	presence					1394:1401	the presence	1390:1401	the presence of CH vibrations from the ether in the fiber	1390:1446	The FTIR analysis showed that the presence of CH vibrations from the ether in the fiber.
34971644	10	82	from	ether	1429:1433	arg1	vibrations					1409:1418	CH vibrations	1406:1418	CH vibrations from the ether	1406:1433	The FTIR analysis showed that the presence of CH vibrations from the ether in the fiber.
34971644	2	83	from	fiber	323:327	arg1	production					270:279	the production	266:279	the production of nanocellulose from Agave gigantea (AG) fiber using the chemical-ultrafine grinding treatment	266:375	This study reports the production of nanocellulose from Agave gigantea (AG) fiber using the chemical-ultrafine grinding treatment.
34971644	6	84	theme	%	1065:1065	arg1	content					1050:1056	the cellulose content	1036:1056	the cellulose content of 20.4%	1036:1065	The bleaching treatment increased the crystal index by 48.3% compared to raw AG fiber, along with an increase in the cellulose content of 20.4%.
32613513	6	0	theme	adsorption	725:734	arg1	consistent					751:760	consistent	751:760	consistent	751:760	The adsorption isotherms were consistent with the Freundlich expression.
32613513	6	0	theme	adsorption	725:734	arg1	isotherms					736:744	The adsorption isotherms	721:744	The adsorption isotherms	721:744	The adsorption isotherms were consistent with the Freundlich expression.
32613513	5	1	from	mesopores	594:602	arg1	transfer					638:645	the adsorbent facilitated mass transfer	607:645	the adsorbent facilitated mass transfer	607:645	The large proportion of mesopores in the adsorbent facilitated mass transfer, and thereby adsorption equilibrium states could be achieved within 15 s.
32613513	10	2	theme	system	1427:1432	arg1	energy					1417:1422	the energy	1413:1422	the energy of system	1413:1432	DFT calculation results showed that the amino and hydroxyl groups in adjacent chitosan units could be tri-coordinated with Pb2+, and the energy of system was greatly decreased due to the coordination interaction.
32613513	5	3	theme	mesopores	594:602	arg1	proportion					580:589	The large proportion	570:589	The large proportion of mesopores in the adsorbent facilitated mass transfer	570:645	The large proportion of mesopores in the adsorbent facilitated mass transfer, and thereby adsorption equilibrium states could be achieved within 15 s.
32613513	5	3	theme	mesopores	594:602	arg1	states					683:688	adsorption equilibrium states	660:688	adsorption equilibrium states	660:688	The large proportion of mesopores in the adsorbent facilitated mass transfer, and thereby adsorption equilibrium states could be achieved within 15 s.
32613513	8	4	theme	- 18.4	1040:1045	arg1	range					1031:1035	the range	1027:1035	the range of - 18.4 to - 16.7 kJ/mol	1027:1062	The adsorption enthalpy change was measured to be - 28.6 kJ/mol and Gibbs free energy change was in the range of - 18.4 to - 16.7 kJ/mol, indicating that this adsorption process was exothermic and spontaneous.
32613513	5	5	from	transfer	638:645	arg1	proportion					580:589	The large proportion	570:589	The large proportion of mesopores in the adsorbent facilitated mass transfer	570:645	The large proportion of mesopores in the adsorbent facilitated mass transfer, and thereby adsorption equilibrium states could be achieved within 15 s.
32613513	5	5	from	transfer	638:645	arg1	states					683:688	adsorption equilibrium states	660:688	adsorption equilibrium states	660:688	The large proportion of mesopores in the adsorbent facilitated mass transfer, and thereby adsorption equilibrium states could be achieved within 15 s.
32613513	4	6	theme	chitosan	517:524	arg1	dispersion					503:512	The good dispersion	494:512	The good dispersion of chitosan	494:524	The good dispersion of chitosan played a crucial role in the high capacity.
32613513	5	7	theme	adsorption	660:669	arg1	states					683:688	adsorption equilibrium states	660:688	adsorption equilibrium states	660:688	The large proportion of mesopores in the adsorbent facilitated mass transfer, and thereby adsorption equilibrium states could be achieved within 15 s.
32613513	2	8	theme	amount	302:307	arg1	effect					284:289	The strong promotion effect	263:289	The strong promotion effect of a small amount of highly dispersed chitosan on the Pb2+ adsorption capacity of clay minerals	263:385	The strong promotion effect of a small amount of highly dispersed chitosan on the Pb2+ adsorption capacity of clay minerals was unveiled.
32613513	10	9	theme	adjacent	1349:1356	arg1	units					1367:1371	adjacent chitosan units	1349:1371	adjacent chitosan units	1349:1371	DFT calculation results showed that the amino and hydroxyl groups in adjacent chitosan units could be tri-coordinated with Pb2+, and the energy of system was greatly decreased due to the coordination interaction.
32613513	0	10	theme	lead	92:95	arg1	ions					97:100	lead ions	92:100	lead ions	92:100	Fabrication of composites with ultra-low chitosan loadings and the adsorption mechanism for lead ions.
32613513	5	11	theme	equilibrium	671:681	arg1	states					683:688	adsorption equilibrium states	660:688	adsorption equilibrium states	660:688	The large proportion of mesopores in the adsorbent facilitated mass transfer, and thereby adsorption equilibrium states could be achieved within 15 s.
32613513	8	12	theme	energy	1006:1011	arg1	change					1013:1018	Gibbs free energy change	995:1018	Gibbs free energy change	995:1018	The adsorption enthalpy change was measured to be - 28.6 kJ/mol and Gibbs free energy change was in the range of - 18.4 to - 16.7 kJ/mol, indicating that this adsorption process was exothermic and spontaneous.
32613513	2	13	theme	small	296:300	arg1	amount					302:307	a small amount	294:307	a small amount of highly dispersed chitosan	294:336	The strong promotion effect of a small amount of highly dispersed chitosan on the Pb2+ adsorption capacity of clay minerals was unveiled.
32613513	2	13	theme	small	296:300	arg1	chitosan					329:336	highly dispersed chitosan	312:336	highly dispersed chitosan	312:336	The strong promotion effect of a small amount of highly dispersed chitosan on the Pb2+ adsorption capacity of clay minerals was unveiled.
32613513	8	14	theme	Gibbs	995:999	arg1	change					1013:1018	Gibbs free energy change	995:1018	Gibbs free energy change	995:1018	The adsorption enthalpy change was measured to be - 28.6 kJ/mol and Gibbs free energy change was in the range of - 18.4 to - 16.7 kJ/mol, indicating that this adsorption process was exothermic and spontaneous.
32613513	6	15	with	consistent	751:760	arg1	expression					782:791	the Freundlich expression	767:791	the Freundlich expression	767:791	The adsorption isotherms were consistent with the Freundlich expression.
32613513	5	16	from	proportion	580:589	arg1	transfer					638:645	the adsorbent facilitated mass transfer	607:645	the adsorbent facilitated mass transfer	607:645	The large proportion of mesopores in the adsorbent facilitated mass transfer, and thereby adsorption equilibrium states could be achieved within 15 s.
32613513	5	17	theme	facilitated	621:631	arg1	transfer					638:645	the adsorbent facilitated mass transfer	607:645	the adsorbent facilitated mass transfer	607:645	The large proportion of mesopores in the adsorbent facilitated mass transfer, and thereby adsorption equilibrium states could be achieved within 15 s.
32613513	5	18	theme	adsorbent	611:619	arg1	transfer					638:645	the adsorbent facilitated mass transfer	607:645	the adsorbent facilitated mass transfer	607:645	The large proportion of mesopores in the adsorbent facilitated mass transfer, and thereby adsorption equilibrium states could be achieved within 15 s.
32613513	10	19	theme	calculation	1284:1294	arg1	results					1296:1302	DFT calculation results	1280:1302	DFT calculation results	1280:1302	DFT calculation results showed that the amino and hydroxyl groups in adjacent chitosan units could be tri-coordinated with Pb2+, and the energy of system was greatly decreased due to the coordination interaction.
32613513	9	20	theme	amino	1184:1188	arg1	groups					1190:1195	the amino groups	1180:1195	the amino groups on chitosan	1180:1207	The FTIR and XPS results demonstrated that the amino groups on chitosan could bond with Pb2+, and contributed to the high adsorption capacity.
32613513	4	21	theme	good	498:501	arg1	dispersion					503:512	The good dispersion	494:512	The good dispersion of chitosan	494:524	The good dispersion of chitosan played a crucial role in the high capacity.
32613513	5	22	theme	mass	633:636	arg1	transfer					638:645	the adsorbent facilitated mass transfer	607:645	the adsorbent facilitated mass transfer	607:645	The large proportion of mesopores in the adsorbent facilitated mass transfer, and thereby adsorption equilibrium states could be achieved within 15 s.
32613513	9	23	theme	FTIR	1141:1144	arg1	results					1154:1160	The FTIR and XPS results	1137:1160	The FTIR and XPS results	1137:1160	The FTIR and XPS results demonstrated that the amino groups on chitosan could bond with Pb2+, and contributed to the high adsorption capacity.
32613513	7	24	theme	Ca2+	867:870	arg1	addition					847:854	the addition	843:854	the addition of 150 ppm Ca2+	843:870	The Pb2+ adsorption capacity was suppressed with the addition of 150 ppm Ca2+ and almost eliminated in the presence of 150 ppm Mg2+.
32613513	9	25	with	bond	1215:1218	arg1	Pb2+					1225:1228	Pb2+	1225:1228	Pb2+	1225:1228	The FTIR and XPS results demonstrated that the amino groups on chitosan could bond with Pb2+, and contributed to the high adsorption capacity.
32613513	5	26	theme	large	574:578	arg1	proportion					580:589	The large proportion	570:589	The large proportion of mesopores in the adsorbent facilitated mass transfer	570:645	The large proportion of mesopores in the adsorbent facilitated mass transfer, and thereby adsorption equilibrium states could be achieved within 15 s.
32613513	2	27	theme	chitosan	329:336	arg1	amount					302:307	a small amount	294:307	a small amount of highly dispersed chitosan	294:336	The strong promotion effect of a small amount of highly dispersed chitosan on the Pb2+ adsorption capacity of clay minerals was unveiled.
32613513	2	27	theme	chitosan	329:336	arg1	chitosan					329:336	highly dispersed chitosan	312:336	highly dispersed chitosan	312:336	The strong promotion effect of a small amount of highly dispersed chitosan on the Pb2+ adsorption capacity of clay minerals was unveiled.
32613513	3	28	theme	0.4 wt	428:433	arg1	%					434:434	0.4 wt%	428:434	0.4 wt%	428:434	With a chitosan loading of 0.4 wt%, the experimental adsorption capacity reached 261.3 mg/g.
32613513	4	29	theme	high	555:558	arg1	capacity					560:567	the high capacity	551:567	the high capacity	551:567	The good dispersion of chitosan played a crucial role in the high capacity.
32613513	2	30	theme	dispersed	319:327	arg1	chitosan					329:336	highly dispersed chitosan	312:336	highly dispersed chitosan	312:336	The strong promotion effect of a small amount of highly dispersed chitosan on the Pb2+ adsorption capacity of clay minerals was unveiled.
32613513	10	31	theme	amino	1320:1324	arg1	groups					1339:1344	the amino and hydroxyl groups	1316:1344	groups	1339:1344	DFT calculation results showed that the amino and hydroxyl groups in adjacent chitosan units could be tri-coordinated with Pb2+, and the energy of system was greatly decreased due to the coordination interaction.
32613513	3	32	theme	%	434:434	arg1	loading					417:423	a chitosan loading	406:423	a chitosan loading of 0.4 wt%	406:434	With a chitosan loading of 0.4 wt%, the experimental adsorption capacity reached 261.3 mg/g.
32613513	8	33	theme	adsorption	1086:1095	arg1	process					1097:1103	this adsorption process	1081:1103	this adsorption process	1081:1103	The adsorption enthalpy change was measured to be - 28.6 kJ/mol and Gibbs free energy change was in the range of - 18.4 to - 16.7 kJ/mol, indicating that this adsorption process was exothermic and spontaneous.
32613513	8	34	theme	free	1001:1004	arg1	change					1013:1018	Gibbs free energy change	995:1018	Gibbs free energy change	995:1018	The adsorption enthalpy change was measured to be - 28.6 kJ/mol and Gibbs free energy change was in the range of - 18.4 to - 16.7 kJ/mol, indicating that this adsorption process was exothermic and spontaneous.
32613513	7	35	theme	150 ppm	859:865	arg1	Ca2+					867:870	150 ppm Ca2+	859:870	150 ppm Ca2+	859:870	The Pb2+ adsorption capacity was suppressed with the addition of 150 ppm Ca2+ and almost eliminated in the presence of 150 ppm Mg2+.
32613513	0	36	theme	composites	15:24	arg1	mechanism					78:86	the adsorption mechanism	63:86	the adsorption mechanism for lead ions	63:100	Fabrication of composites with ultra-low chitosan loadings and the adsorption mechanism for lead ions.
32613513	0	36	theme	composites	15:24	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of composites with ultra-low chitosan loadings	0:57	Fabrication of composites with ultra-low chitosan loadings and the adsorption mechanism for lead ions.
32613513	9	37	from	groups	1190:1195	arg1	chitosan					1200:1207	chitosan	1200:1207	chitosan	1200:1207	The FTIR and XPS results demonstrated that the amino groups on chitosan could bond with Pb2+, and contributed to the high adsorption capacity.
32613513	2	38	from	effect	284:289	arg1	capacity					361:368	the Pb2+ adsorption capacity	341:368	the Pb2+ adsorption capacity of clay minerals	341:385	The strong promotion effect of a small amount of highly dispersed chitosan on the Pb2+ adsorption capacity of clay minerals was unveiled.
32613513	1	39	theme	organic/inorganic	207:223	arg1	composite					232:240	an organic/inorganic hybrid composite	204:240	an organic/inorganic hybrid composite	204:240	Through a facile impregnation-precipitation strategy, chitosan was dispersed on bentonite to prepare an organic/inorganic hybrid composite for Pb2+ adsorption.
32613513	0	40	theme	chitosan	41:48	arg1	loadings					50:57	ultra-low chitosan loadings	31:57	ultra-low chitosan loadings	31:57	Fabrication of composites with ultra-low chitosan loadings and the adsorption mechanism for lead ions.
32613513	2	41	theme	Pb2+	345:348	arg1	capacity					361:368	the Pb2+ adsorption capacity	341:368	the Pb2+ adsorption capacity of clay minerals	341:385	The strong promotion effect of a small amount of highly dispersed chitosan on the Pb2+ adsorption capacity of clay minerals was unveiled.
32613513	3	42	theme	experimental	441:452	arg1	capacity					465:472	the experimental adsorption capacity	437:472	the experimental adsorption capacity	437:472	With a chitosan loading of 0.4 wt%, the experimental adsorption capacity reached 261.3 mg/g.
32613513	10	43	from	groups	1339:1344	arg1	units					1367:1371	adjacent chitosan units	1349:1371	adjacent chitosan units	1349:1371	DFT calculation results showed that the amino and hydroxyl groups in adjacent chitosan units could be tri-coordinated with Pb2+, and the energy of system was greatly decreased due to the coordination interaction.
32613513	1	44	theme	hybrid	225:230	arg1	composite					232:240	an organic/inorganic hybrid composite	204:240	an organic/inorganic hybrid composite	204:240	Through a facile impregnation-precipitation strategy, chitosan was dispersed on bentonite to prepare an organic/inorganic hybrid composite for Pb2+ adsorption.
32613513	0	45	theme	ultra-low	31:39	arg1	loadings					50:57	ultra-low chitosan loadings	31:57	ultra-low chitosan loadings	31:57	Fabrication of composites with ultra-low chitosan loadings and the adsorption mechanism for lead ions.
32613513	4	46	theme	crucial	535:541	arg1	role					543:546	a crucial role	533:546	a crucial role	533:546	The good dispersion of chitosan played a crucial role in the high capacity.
32613513	10	47	theme	coordination	1467:1478	arg1	interaction					1480:1490	the coordination interaction	1463:1490	the coordination interaction	1463:1490	DFT calculation results showed that the amino and hydroxyl groups in adjacent chitosan units could be tri-coordinated with Pb2+, and the energy of system was greatly decreased due to the coordination interaction.
32613513	8	48	theme	enthalpy	942:949	arg1	change					951:956	The adsorption enthalpy change	927:956	The adsorption enthalpy change	927:956	The adsorption enthalpy change was measured to be - 28.6 kJ/mol and Gibbs free energy change was in the range of - 18.4 to - 16.7 kJ/mol, indicating that this adsorption process was exothermic and spontaneous.
32613513	8	48	theme	enthalpy	942:949	arg1	- 28.6 kJ/mol					977:989	- 28.6 kJ/mol	977:989	- 28.6 kJ/mol	977:989	The adsorption enthalpy change was measured to be - 28.6 kJ/mol and Gibbs free energy change was in the range of - 18.4 to - 16.7 kJ/mol, indicating that this adsorption process was exothermic and spontaneous.
32613513	3	49	theme	adsorption	454:463	arg1	capacity					465:472	the experimental adsorption capacity	437:472	the experimental adsorption capacity	437:472	With a chitosan loading of 0.4 wt%, the experimental adsorption capacity reached 261.3 mg/g.
32613513	10	50	theme	DFT	1280:1282	arg1	results					1296:1302	DFT calculation results	1280:1302	DFT calculation results	1280:1302	DFT calculation results showed that the amino and hydroxyl groups in adjacent chitosan units could be tri-coordinated with Pb2+, and the energy of system was greatly decreased due to the coordination interaction.
32613513	7	51	theme	Pb2+	798:801	arg1	capacity					814:821	The Pb2+ adsorption capacity	794:821	The Pb2+ adsorption capacity	794:821	The Pb2+ adsorption capacity was suppressed with the addition of 150 ppm Ca2+ and almost eliminated in the presence of 150 ppm Mg2+.
32613513	0	52	with	composites	15:24	arg1	loadings					50:57	ultra-low chitosan loadings	31:57	ultra-low chitosan loadings	31:57	Fabrication of composites with ultra-low chitosan loadings and the adsorption mechanism for lead ions.
32613513	2	53	theme	adsorption	350:359	arg1	capacity					361:368	the Pb2+ adsorption capacity	341:368	the Pb2+ adsorption capacity of clay minerals	341:385	The strong promotion effect of a small amount of highly dispersed chitosan on the Pb2+ adsorption capacity of clay minerals was unveiled.
32613513	3	54	theme	chitosan	408:415	arg1	loading					417:423	a chitosan loading	406:423	a chitosan loading of 0.4 wt%	406:434	With a chitosan loading of 0.4 wt%, the experimental adsorption capacity reached 261.3 mg/g.
32613513	10	55	theme	hydroxyl	1330:1337	arg1	groups					1339:1344	the amino and hydroxyl groups	1316:1344	groups	1339:1344	DFT calculation results showed that the amino and hydroxyl groups in adjacent chitosan units could be tri-coordinated with Pb2+, and the energy of system was greatly decreased due to the coordination interaction.
32613513	1	56	theme	facile	113:118	arg1	strategy					147:154	a facile impregnation-precipitation strategy	111:154	a facile impregnation-precipitation strategy	111:154	Through a facile impregnation-precipitation strategy, chitosan was dispersed on bentonite to prepare an organic/inorganic hybrid composite for Pb2+ adsorption.
32613513	8	57	theme	adsorption	931:940	arg1	change					951:956	The adsorption enthalpy change	927:956	The adsorption enthalpy change	927:956	The adsorption enthalpy change was measured to be - 28.6 kJ/mol and Gibbs free energy change was in the range of - 18.4 to - 16.7 kJ/mol, indicating that this adsorption process was exothermic and spontaneous.
32613513	8	57	theme	adsorption	931:940	arg1	- 28.6 kJ/mol					977:989	- 28.6 kJ/mol	977:989	- 28.6 kJ/mol	977:989	The adsorption enthalpy change was measured to be - 28.6 kJ/mol and Gibbs free energy change was in the range of - 18.4 to - 16.7 kJ/mol, indicating that this adsorption process was exothermic and spontaneous.
32613513	9	58	theme	high	1254:1257	arg1	capacity					1270:1277	the high adsorption capacity	1250:1277	the high adsorption capacity	1250:1277	The FTIR and XPS results demonstrated that the amino groups on chitosan could bond with Pb2+, and contributed to the high adsorption capacity.
32613513	9	59	theme	XPS	1150:1152	arg1	results					1154:1160	The FTIR and XPS results	1137:1160	The FTIR and XPS results	1137:1160	The FTIR and XPS results demonstrated that the amino groups on chitosan could bond with Pb2+, and contributed to the high adsorption capacity.
32613513	1	60	theme	impregnation-precipitation	120:145	arg1	strategy					147:154	a facile impregnation-precipitation strategy	111:154	a facile impregnation-precipitation strategy	111:154	Through a facile impregnation-precipitation strategy, chitosan was dispersed on bentonite to prepare an organic/inorganic hybrid composite for Pb2+ adsorption.
32613513	6	61	theme	Freundlich	771:780	arg1	expression					782:791	the Freundlich expression	767:791	the Freundlich expression	767:791	The adsorption isotherms were consistent with the Freundlich expression.
32613513	9	62	theme	adsorption	1259:1268	arg1	capacity					1270:1277	the high adsorption capacity	1250:1277	the high adsorption capacity	1250:1277	The FTIR and XPS results demonstrated that the amino groups on chitosan could bond with Pb2+, and contributed to the high adsorption capacity.
32613513	2	63	theme	promotion	274:282	arg1	effect					284:289	The strong promotion effect	263:289	The strong promotion effect of a small amount of highly dispersed chitosan on the Pb2+ adsorption capacity of clay minerals	263:385	The strong promotion effect of a small amount of highly dispersed chitosan on the Pb2+ adsorption capacity of clay minerals was unveiled.
32613513	7	64	theme	150 ppm	913:919	arg1	Mg2+					921:924	150 ppm Mg2+	913:924	150 ppm Mg2+	913:924	The Pb2+ adsorption capacity was suppressed with the addition of 150 ppm Ca2+ and almost eliminated in the presence of 150 ppm Mg2+.
32613513	1	65	theme	Pb2+	246:249	arg1	adsorption					251:260	Pb2+ adsorption	246:260	Pb2+ adsorption	246:260	Through a facile impregnation-precipitation strategy, chitosan was dispersed on bentonite to prepare an organic/inorganic hybrid composite for Pb2+ adsorption.
32613513	0	66	theme	adsorption	67:76	arg1	mechanism					78:86	the adsorption mechanism	63:86	the adsorption mechanism for lead ions	63:100	Fabrication of composites with ultra-low chitosan loadings and the adsorption mechanism for lead ions.
32613513	2	67	theme	strong	267:272	arg1	effect					284:289	The strong promotion effect	263:289	The strong promotion effect of a small amount of highly dispersed chitosan on the Pb2+ adsorption capacity of clay minerals	263:385	The strong promotion effect of a small amount of highly dispersed chitosan on the Pb2+ adsorption capacity of clay minerals was unveiled.
32613513	2	68	theme	minerals	378:385	arg1	capacity					361:368	the Pb2+ adsorption capacity	341:368	the Pb2+ adsorption capacity of clay minerals	341:385	The strong promotion effect of a small amount of highly dispersed chitosan on the Pb2+ adsorption capacity of clay minerals was unveiled.
32613513	7	69	theme	Mg2+	921:924	arg1	presence					901:908	the presence	897:908	the presence of 150 ppm Mg2+	897:924	The Pb2+ adsorption capacity was suppressed with the addition of 150 ppm Ca2+ and almost eliminated in the presence of 150 ppm Mg2+.
32613513	5	70	from	states	683:688	arg1	transfer					638:645	the adsorbent facilitated mass transfer	607:645	the adsorbent facilitated mass transfer	607:645	The large proportion of mesopores in the adsorbent facilitated mass transfer, and thereby adsorption equilibrium states could be achieved within 15 s.
32613513	10	71	theme	chitosan	1358:1365	arg1	units					1367:1371	adjacent chitosan units	1349:1371	adjacent chitosan units	1349:1371	DFT calculation results showed that the amino and hydroxyl groups in adjacent chitosan units could be tri-coordinated with Pb2+, and the energy of system was greatly decreased due to the coordination interaction.
32613513	2	72	theme	clay	373:376	arg1	minerals					378:385	clay minerals	373:385	clay minerals	373:385	The strong promotion effect of a small amount of highly dispersed chitosan on the Pb2+ adsorption capacity of clay minerals was unveiled.
32613513	7	73	theme	adsorption	803:812	arg1	capacity					814:821	The Pb2+ adsorption capacity	794:821	The Pb2+ adsorption capacity	794:821	The Pb2+ adsorption capacity was suppressed with the addition of 150 ppm Ca2+ and almost eliminated in the presence of 150 ppm Mg2+.
33233241	0	0	theme	simulated	96:104	arg1	conditions					123:132	simulated gastrointestinal conditions	96:132	simulated gastrointestinal conditions	96:132	Digestion of animal- and plant-based proteins encapsulated in κ-carrageenan/protein beads under simulated gastrointestinal conditions.
33233241	2	1	theme	solutions	506:514	arg1	behavior					467:474	the shear thinning behavior	448:474	the shear thinning behavior of the protein/polysaccharide solutions used to form them	448:532	Bead dimensions were measured using a digital caliper and found to increase as the shear thinning behavior of the protein/polysaccharide solutions used to form them increased.
33233241	1	2	theme	animal-derived	171:184	arg1	proteins					225:232	both animal-derived (whey) and plant-derived (soy and pea) proteins	166:232	both animal-derived (whey) and plant-derived (soy and pea) proteins	166:232	In this study, we encapsulated both animal-derived (whey) and plant-derived (soy and pea) proteins within polysaccharide/protein beads and measured the digestion of these beads under simulated gastrointestinal tract (GIT) conditions.
33233241	2	3	theme	protein/polysaccharide	483:504	arg1	solutions					506:514	the protein/polysaccharide solutions	479:514	the protein/polysaccharide solutions used to form them	479:532	Bead dimensions were measured using a digital caliper and found to increase as the shear thinning behavior of the protein/polysaccharide solutions used to form them increased.
33233241	3	4	theme	GIT	721:723	arg1	conditions					725:734	simulated static GIT conditions	704:734	simulated static GIT conditions	704:734	The hydrolysis of the plant and animal proteins trapped inside the protein/polysaccharide beads was studied using the pH-stat automatic titration method under simulated static GIT conditions.
33233241	9	5	theme	proteins	1646:1653	arg1	behavior					1634:1641	the gastrointestinal behavior	1613:1641	the gastrointestinal behavior of proteins	1613:1653	Our results indicate that the gastrointestinal behavior of proteins can be modulated by encapsulating them in polysaccharide beads, which may be useful for the design of certain types of functional foods.
33233241	6	6	theme	intestine	1101:1109	arg1	phase					1111:1115	the small intestine phase	1091:1115	the small intestine phase	1091:1115	Indeed, by the completion of the small intestine phase, only "ghost" beads remained that contained cross-linked polysaccharides.
33233241	8	7	theme	electron	1460:1467	arg1	microscopy					1469:1478	scanning electron microscopy	1451:1478	scanning electron microscopy	1451:1478	Raman spectroscopy and scanning electron microscopy showed that there were changes in the composition and structure of the beads throughout the simulated GIT.
33233241	5	8	theme	intestinal	973:982	arg1	conditions					984:993	small intestinal conditions	967:993	small intestinal conditions	967:993	Conversely, they were almost fully digested under small intestinal conditions, with around 87 to 97% of the protein being hydrolyzed by the end.
33233241	0	9	theme	gastrointestinal	106:121	arg1	conditions					123:132	simulated gastrointestinal conditions	96:132	simulated gastrointestinal conditions	96:132	Digestion of animal- and plant-based proteins encapsulated in κ-carrageenan/protein beads under simulated gastrointestinal conditions.
33233241	4	10	theme	gastric	808:814	arg1	conditions					816:825	gastric conditions	808:825	gastric conditions	808:825	The encapsulated proteins were relatively resistant to digestion under gastric conditions, with only about 10 to 13% of the protein being digested by the end of the stomach phase.
33233241	8	11	from	changes	1503:1509	arg1	structure					1534:1542	structure	1534:1542	structure	1534:1542	Raman spectroscopy and scanning electron microscopy showed that there were changes in the composition and structure of the beads throughout the simulated GIT.
33233241	8	11	from	changes	1503:1509	arg1	composition					1518:1528	composition	1518:1528	composition	1518:1528	Raman spectroscopy and scanning electron microscopy showed that there were changes in the composition and structure of the beads throughout the simulated GIT.
33233241	2	12	theme	Bead	369:372	arg1	dimensions					374:383	Bead dimensions	369:383	Bead dimensions	369:383	Bead dimensions were measured using a digital caliper and found to increase as the shear thinning behavior of the protein/polysaccharide solutions used to form them increased.
33233241	7	13	theme	stomach	1276:1282	arg1	p < 0.05					1291:1298	p < 0.05	1291:1298	p < 0.05	1291:1298	The soy and pea proteins were digested slightly faster than the whey proteins in the stomach phase (p < 0.05), whereas the pea proteins were digested slightly faster than the soy and whey proteins under intestinal conditions (p < 0.05).
33233241	7	13	theme	stomach	1276:1282	arg1	phase					1284:1288	the stomach phase	1272:1288	the stomach phase (p < 0.05)	1272:1299	The soy and pea proteins were digested slightly faster than the whey proteins in the stomach phase (p < 0.05), whereas the pea proteins were digested slightly faster than the soy and whey proteins under intestinal conditions (p < 0.05).
33233241	2	14	theme	digital	407:413	arg1	caliper					415:421	a digital caliper	405:421	a digital caliper	405:421	Bead dimensions were measured using a digital caliper and found to increase as the shear thinning behavior of the protein/polysaccharide solutions used to form them increased.
33233241	7	15	theme	intestinal	1394:1403	arg1	p < 0.05					1417:1424	p < 0.05	1417:1424	p < 0.05	1417:1424	The soy and pea proteins were digested slightly faster than the whey proteins in the stomach phase (p < 0.05), whereas the pea proteins were digested slightly faster than the soy and whey proteins under intestinal conditions (p < 0.05).
33233241	7	15	theme	intestinal	1394:1403	arg1	conditions					1405:1414	intestinal conditions	1394:1414	intestinal conditions (p < 0.05)	1394:1425	The soy and pea proteins were digested slightly faster than the whey proteins in the stomach phase (p < 0.05), whereas the pea proteins were digested slightly faster than the soy and whey proteins under intestinal conditions (p < 0.05).
33233241	1	16	link	plant-derived	197:209	arg1	proteins					225:232	both animal-derived (whey) and plant-derived (soy and pea) proteins	166:232	both animal-derived (whey) and plant-derived (soy and pea) proteins	166:232	In this study, we encapsulated both animal-derived (whey) and plant-derived (soy and pea) proteins within polysaccharide/protein beads and measured the digestion of these beads under simulated gastrointestinal tract (GIT) conditions.
33233241	10	17	theme	animal-based	1843:1854	arg1	proteins					1856:1863	the plant-based and animal-based proteins	1823:1863	the plant-based and animal-based proteins	1823:1863	Even so, the beads formed from the plant-based and animal-based proteins behaved fairly similarly, suggesting that animal proteins could be replaced by plant-based ones for this purpose.
33233241	6	18	theme	"	1129:1129	arg1	beads					1131:1135	ghost" beads	1124:1135	only "ghost" beads	1118:1135	Indeed, by the completion of the small intestine phase, only "ghost" beads remained that contained cross-linked polysaccharides.
33233241	10	19	theme	plant-based	1827:1837	arg1	proteins					1856:1863	the plant-based and animal-based proteins	1823:1863	the plant-based and animal-based proteins	1823:1863	Even so, the beads formed from the plant-based and animal-based proteins behaved fairly similarly, suggesting that animal proteins could be replaced by plant-based ones for this purpose.
33233241	2	20	theme	shear	452:456	arg1	behavior					467:474	the shear thinning behavior	448:474	the shear thinning behavior of the protein/polysaccharide solutions used to form them	448:532	Bead dimensions were measured using a digital caliper and found to increase as the shear thinning behavior of the protein/polysaccharide solutions used to form them increased.
33233241	6	21	theme	ghost	1124:1128	arg1	beads					1131:1135	ghost" beads	1124:1135	only "ghost" beads	1118:1135	Indeed, by the completion of the small intestine phase, only "ghost" beads remained that contained cross-linked polysaccharides.
33233241	7	22	theme	soy	1195:1197	arg1	proteins					1207:1214	The soy and pea proteins	1191:1214	proteins	1207:1214	The soy and pea proteins were digested slightly faster than the whey proteins in the stomach phase (p < 0.05), whereas the pea proteins were digested slightly faster than the soy and whey proteins under intestinal conditions (p < 0.05).
33233241	1	23	dep	animal-derived	171:184	arg1	whey					187:190	whey	187:190	whey	187:190	In this study, we encapsulated both animal-derived (whey) and plant-derived (soy and pea) proteins within polysaccharide/protein beads and measured the digestion of these beads under simulated gastrointestinal tract (GIT) conditions.
33233241	9	24	theme	certain	1757:1763	arg1	types					1765:1769	certain types	1757:1769	certain types of functional foods	1757:1789	Our results indicate that the gastrointestinal behavior of proteins can be modulated by encapsulating them in polysaccharide beads, which may be useful for the design of certain types of functional foods.
33233241	3	25	theme	proteins	584:591	arg1	hydrolysis					549:558	The hydrolysis	545:558	The hydrolysis of the plant and animal proteins trapped inside the protein/polysaccharide beads	545:639	The hydrolysis of the plant and animal proteins trapped inside the protein/polysaccharide beads was studied using the pH-stat automatic titration method under simulated static GIT conditions.
33233241	1	26	theme	plant-derived	197:209	arg1	proteins					225:232	both animal-derived (whey) and plant-derived (soy and pea) proteins	166:232	both animal-derived (whey) and plant-derived (soy and pea) proteins	166:232	In this study, we encapsulated both animal-derived (whey) and plant-derived (soy and pea) proteins within polysaccharide/protein beads and measured the digestion of these beads under simulated gastrointestinal tract (GIT) conditions.
33233241	9	27	theme	types	1765:1769	arg1	design					1747:1752	the design	1743:1752	the design of certain types of functional foods	1743:1789	Our results indicate that the gastrointestinal behavior of proteins can be modulated by encapsulating them in polysaccharide beads, which may be useful for the design of certain types of functional foods.
33233241	7	28	theme	soy	1366:1368	arg1	proteins					1379:1386	the soy and whey proteins	1362:1386	proteins	1379:1386	The soy and pea proteins were digested slightly faster than the whey proteins in the stomach phase (p < 0.05), whereas the pea proteins were digested slightly faster than the soy and whey proteins under intestinal conditions (p < 0.05).
33233241	1	29	link	animal-derived	171:184	arg1	proteins					225:232	both animal-derived (whey) and plant-derived (soy and pea) proteins	166:232	both animal-derived (whey) and plant-derived (soy and pea) proteins	166:232	In this study, we encapsulated both animal-derived (whey) and plant-derived (soy and pea) proteins within polysaccharide/protein beads and measured the digestion of these beads under simulated gastrointestinal tract (GIT) conditions.
33233241	3	30	theme	animal	577:582	arg1	proteins					584:591	the plant and animal proteins	563:591	the plant and animal proteins trapped inside the protein/polysaccharide beads	563:639	The hydrolysis of the plant and animal proteins trapped inside the protein/polysaccharide beads was studied using the pH-stat automatic titration method under simulated static GIT conditions.
33233241	3	31	theme	plant	567:571	arg1	proteins					584:591	the plant and animal proteins	563:591	the plant and animal proteins trapped inside the protein/polysaccharide beads	563:639	The hydrolysis of the plant and animal proteins trapped inside the protein/polysaccharide beads was studied using the pH-stat automatic titration method under simulated static GIT conditions.
33233241	5	32	theme	protein	1025:1031	arg1	%					1016:1016	around 87 to 97%	1001:1016	around 87 to 97% of the protein	1001:1031	Conversely, they were almost fully digested under small intestinal conditions, with around 87 to 97% of the protein being hydrolyzed by the end.
33233241	5	32	theme	protein	1025:1031	arg1	protein					1025:1031	the protein	1021:1031	the protein	1021:1031	Conversely, they were almost fully digested under small intestinal conditions, with around 87 to 97% of the protein being hydrolyzed by the end.
33233241	0	33	theme	animal-	13:19	arg1	Digestion					0:8	Digestion	0:8	Digestion of animal- and plant-based proteins	0:44	Digestion of animal- and plant-based proteins encapsulated in κ-carrageenan/protein beads under simulated gastrointestinal conditions.
33233241	10	34	theme	animal	1907:1912	arg1	proteins					1914:1921	animal proteins	1907:1921	animal proteins	1907:1921	Even so, the beads formed from the plant-based and animal-based proteins behaved fairly similarly, suggesting that animal proteins could be replaced by plant-based ones for this purpose.
33233241	3	35	theme	automatic	671:679	arg1	method					691:696	the pH-stat automatic titration method	659:696	the pH-stat automatic titration method	659:696	The hydrolysis of the plant and animal proteins trapped inside the protein/polysaccharide beads was studied using the pH-stat automatic titration method under simulated static GIT conditions.
33233241	5	36	theme	small	967:971	arg1	conditions					984:993	small intestinal conditions	967:993	small intestinal conditions	967:993	Conversely, they were almost fully digested under small intestinal conditions, with around 87 to 97% of the protein being hydrolyzed by the end.
33233241	5	37	dep	97	1014:1015	arg1	to					1011:1012	to	1011:1012	to	1011:1012	Conversely, they were almost fully digested under small intestinal conditions, with around 87 to 97% of the protein being hydrolyzed by the end.
33233241	0	38	theme	proteins	37:44	arg1	Digestion					0:8	Digestion	0:8	Digestion of animal- and plant-based proteins	0:44	Digestion of animal- and plant-based proteins encapsulated in κ-carrageenan/protein beads under simulated gastrointestinal conditions.
33233241	1	39	theme	beads	306:310	arg1	digestion					287:295	the digestion	283:295	the digestion of these beads	283:310	In this study, we encapsulated both animal-derived (whey) and plant-derived (soy and pea) proteins within polysaccharide/protein beads and measured the digestion of these beads under simulated gastrointestinal tract (GIT) conditions.
33233241	3	40	theme	titration	681:689	arg1	method					691:696	the pH-stat automatic titration method	659:696	the pH-stat automatic titration method	659:696	The hydrolysis of the plant and animal proteins trapped inside the protein/polysaccharide beads was studied using the pH-stat automatic titration method under simulated static GIT conditions.
33233241	10	41	theme	plant-based	1944:1954	arg1	ones					1956:1959	plant-based ones	1944:1959	plant-based ones for this purpose	1944:1976	Even so, the beads formed from the plant-based and animal-based proteins behaved fairly similarly, suggesting that animal proteins could be replaced by plant-based ones for this purpose.
33233241	0	42	theme	plant-based	25:35	arg1	proteins					37:44	plant-based proteins	25:44	plant-based proteins	25:44	Digestion of animal- and plant-based proteins encapsulated in κ-carrageenan/protein beads under simulated gastrointestinal conditions.
33233241	6	43	link	cross-linked	1161:1172	arg1	polysaccharides					1174:1188	cross-linked polysaccharides	1161:1188	cross-linked polysaccharides	1161:1188	Indeed, by the completion of the small intestine phase, only "ghost" beads remained that contained cross-linked polysaccharides.
33233241	9	44	theme	foods	1785:1789	arg1	types					1765:1769	certain types	1757:1769	certain types of functional foods	1757:1789	Our results indicate that the gastrointestinal behavior of proteins can be modulated by encapsulating them in polysaccharide beads, which may be useful for the design of certain types of functional foods.
33233241	1	45	dep	plant-derived	197:209	arg1	soy					212:214	soy	212:214	soy	212:214	In this study, we encapsulated both animal-derived (whey) and plant-derived (soy and pea) proteins within polysaccharide/protein beads and measured the digestion of these beads under simulated gastrointestinal tract (GIT) conditions.
33233241	1	45	dep	plant-derived	197:209	arg1	pea					220:222	pea	220:222	pea	220:222	In this study, we encapsulated both animal-derived (whey) and plant-derived (soy and pea) proteins within polysaccharide/protein beads and measured the digestion of these beads under simulated gastrointestinal tract (GIT) conditions.
33233241	7	46	from	proteins	1260:1267	arg1	p < 0.05					1291:1298	p < 0.05	1291:1298	p < 0.05	1291:1298	The soy and pea proteins were digested slightly faster than the whey proteins in the stomach phase (p < 0.05), whereas the pea proteins were digested slightly faster than the soy and whey proteins under intestinal conditions (p < 0.05).
33233241	7	46	from	proteins	1260:1267	arg1	phase					1284:1288	the stomach phase	1272:1288	the stomach phase (p < 0.05)	1272:1299	The soy and pea proteins were digested slightly faster than the whey proteins in the stomach phase (p < 0.05), whereas the pea proteins were digested slightly faster than the soy and whey proteins under intestinal conditions (p < 0.05).
33233241	4	47	dep	13	850:851	arg1	to					847:848	to	847:848	to	847:848	The encapsulated proteins were relatively resistant to digestion under gastric conditions, with only about 10 to 13% of the protein being digested by the end of the stomach phase.
33233241	1	48	theme	simulated	318:326	arg1	GIT					352:354	GIT	352:354	GIT	352:354	In this study, we encapsulated both animal-derived (whey) and plant-derived (soy and pea) proteins within polysaccharide/protein beads and measured the digestion of these beads under simulated gastrointestinal tract (GIT) conditions.
33233241	1	48	theme	simulated	318:326	arg1	tract					345:349	simulated gastrointestinal tract	318:349	simulated gastrointestinal tract (GIT) conditions	318:366	In this study, we encapsulated both animal-derived (whey) and plant-derived (soy and pea) proteins within polysaccharide/protein beads and measured the digestion of these beads under simulated gastrointestinal tract (GIT) conditions.
33233241	4	49	theme	phase	910:914	arg1	end					891:893	the end	887:893	the end of the stomach phase	887:914	The encapsulated proteins were relatively resistant to digestion under gastric conditions, with only about 10 to 13% of the protein being digested by the end of the stomach phase.
33233241	3	50	theme	pH-stat	663:669	arg1	method					691:696	the pH-stat automatic titration method	659:696	the pH-stat automatic titration method	659:696	The hydrolysis of the plant and animal proteins trapped inside the protein/polysaccharide beads was studied using the pH-stat automatic titration method under simulated static GIT conditions.
33233241	6	51	theme	phase	1111:1115	arg1	completion					1077:1086	the completion	1073:1086	the completion of the small intestine phase	1073:1115	Indeed, by the completion of the small intestine phase, only "ghost" beads remained that contained cross-linked polysaccharides.
33233241	2	52	theme	thinning	458:465	arg1	behavior					467:474	the shear thinning behavior	448:474	the shear thinning behavior of the protein/polysaccharide solutions used to form them	448:532	Bead dimensions were measured using a digital caliper and found to increase as the shear thinning behavior of the protein/polysaccharide solutions used to form them increased.
33233241	1	53	theme	gastrointestinal	328:343	arg1	GIT					352:354	GIT	352:354	GIT	352:354	In this study, we encapsulated both animal-derived (whey) and plant-derived (soy and pea) proteins within polysaccharide/protein beads and measured the digestion of these beads under simulated gastrointestinal tract (GIT) conditions.
33233241	1	53	theme	gastrointestinal	328:343	arg1	tract					345:349	simulated gastrointestinal tract	318:349	simulated gastrointestinal tract (GIT) conditions	318:366	In this study, we encapsulated both animal-derived (whey) and plant-derived (soy and pea) proteins within polysaccharide/protein beads and measured the digestion of these beads under simulated gastrointestinal tract (GIT) conditions.
33233241	4	54	theme	stomach	902:908	arg1	phase					910:914	the stomach phase	898:914	the stomach phase	898:914	The encapsulated proteins were relatively resistant to digestion under gastric conditions, with only about 10 to 13% of the protein being digested by the end of the stomach phase.
33233241	3	55	theme	simulated	704:712	arg1	conditions					725:734	simulated static GIT conditions	704:734	simulated static GIT conditions	704:734	The hydrolysis of the plant and animal proteins trapped inside the protein/polysaccharide beads was studied using the pH-stat automatic titration method under simulated static GIT conditions.
33233241	4	56	theme	protein	861:867	arg1	%					852:852	only about 10 to 13%	833:852	only about 10 to 13% of the protein	833:867	The encapsulated proteins were relatively resistant to digestion under gastric conditions, with only about 10 to 13% of the protein being digested by the end of the stomach phase.
33233241	4	56	theme	protein	861:867	arg1	protein					861:867	the protein	857:867	the protein	857:867	The encapsulated proteins were relatively resistant to digestion under gastric conditions, with only about 10 to 13% of the protein being digested by the end of the stomach phase.
33233241	9	57	theme	gastrointestinal	1617:1632	arg1	behavior					1634:1641	the gastrointestinal behavior	1613:1641	the gastrointestinal behavior of proteins	1613:1653	Our results indicate that the gastrointestinal behavior of proteins can be modulated by encapsulating them in polysaccharide beads, which may be useful for the design of certain types of functional foods.
33233241	4	58	theme	encapsulated	741:752	arg1	proteins					754:761	The encapsulated proteins	737:761	The encapsulated proteins	737:761	The encapsulated proteins were relatively resistant to digestion under gastric conditions, with only about 10 to 13% of the protein being digested by the end of the stomach phase.
33233241	4	58	theme	encapsulated	741:752	arg1	resistant					779:787	resistant	779:787	resistant	779:787	The encapsulated proteins were relatively resistant to digestion under gastric conditions, with only about 10 to 13% of the protein being digested by the end of the stomach phase.
33233241	1	59	theme	tract	345:349	arg1	conditions					357:366	simulated gastrointestinal tract (GIT) conditions	318:366	simulated gastrointestinal tract (GIT) conditions	318:366	In this study, we encapsulated both animal-derived (whey) and plant-derived (soy and pea) proteins within polysaccharide/protein beads and measured the digestion of these beads under simulated gastrointestinal tract (GIT) conditions.
33233241	9	60	theme	polysaccharide	1697:1710	arg1	beads					1712:1716	polysaccharide beads	1697:1716	polysaccharide beads	1697:1716	Our results indicate that the gastrointestinal behavior of proteins can be modulated by encapsulating them in polysaccharide beads, which may be useful for the design of certain types of functional foods.
33233241	3	61	theme	static	714:719	arg1	conditions					725:734	simulated static GIT conditions	704:734	simulated static GIT conditions	704:734	The hydrolysis of the plant and animal proteins trapped inside the protein/polysaccharide beads was studied using the pH-stat automatic titration method under simulated static GIT conditions.
33233241	3	62	theme	protein/polysaccharide	612:633	arg1	beads					635:639	the protein/polysaccharide beads	608:639	the protein/polysaccharide beads	608:639	The hydrolysis of the plant and animal proteins trapped inside the protein/polysaccharide beads was studied using the pH-stat automatic titration method under simulated static GIT conditions.
33233241	6	63	theme	small	1095:1099	arg1	intestine					1101:1109	the small intestine	1091:1109	the small intestine phase	1091:1115	Indeed, by the completion of the small intestine phase, only "ghost" beads remained that contained cross-linked polysaccharides.
33233241	7	64	dep	digested	1221:1228	arg1	whereas					1302:1308	whereas	1302:1308	whereas	1302:1308	The soy and pea proteins were digested slightly faster than the whey proteins in the stomach phase (p < 0.05), whereas the pea proteins were digested slightly faster than the soy and whey proteins under intestinal conditions (p < 0.05).
33233241	7	65	theme	whey	1374:1377	arg1	proteins					1379:1386	the soy and whey proteins	1362:1386	proteins	1379:1386	The soy and pea proteins were digested slightly faster than the whey proteins in the stomach phase (p < 0.05), whereas the pea proteins were digested slightly faster than the soy and whey proteins under intestinal conditions (p < 0.05).
33233241	7	66	theme	pea	1314:1316	arg1	proteins					1318:1325	the pea proteins	1310:1325	the pea proteins	1310:1325	The soy and pea proteins were digested slightly faster than the whey proteins in the stomach phase (p < 0.05), whereas the pea proteins were digested slightly faster than the soy and whey proteins under intestinal conditions (p < 0.05).
33233241	9	67	theme	functional	1774:1783	arg1	foods					1785:1789	functional foods	1774:1789	functional foods	1774:1789	Our results indicate that the gastrointestinal behavior of proteins can be modulated by encapsulating them in polysaccharide beads, which may be useful for the design of certain types of functional foods.
33233241	8	68	theme	beads	1551:1555	arg1	structure					1534:1542	structure	1534:1542	structure	1534:1542	Raman spectroscopy and scanning electron microscopy showed that there were changes in the composition and structure of the beads throughout the simulated GIT.
33233241	8	68	theme	beads	1551:1555	arg1	composition					1518:1528	composition	1518:1528	composition	1518:1528	Raman spectroscopy and scanning electron microscopy showed that there were changes in the composition and structure of the beads throughout the simulated GIT.
33233241	8	69	theme	Raman	1428:1432	arg1	spectroscopy					1434:1445	Raman spectroscopy	1428:1445	Raman spectroscopy	1428:1445	Raman spectroscopy and scanning electron microscopy showed that there were changes in the composition and structure of the beads throughout the simulated GIT.
33233241	6	70	theme	cross-linked	1161:1172	arg1	polysaccharides					1174:1188	cross-linked polysaccharides	1161:1188	cross-linked polysaccharides	1161:1188	Indeed, by the completion of the small intestine phase, only "ghost" beads remained that contained cross-linked polysaccharides.
33233241	7	71	theme	pea	1203:1205	arg1	proteins					1207:1214	The soy and pea proteins	1191:1214	proteins	1207:1214	The soy and pea proteins were digested slightly faster than the whey proteins in the stomach phase (p < 0.05), whereas the pea proteins were digested slightly faster than the soy and whey proteins under intestinal conditions (p < 0.05).
33233241	8	72	theme	simulated	1572:1580	arg1	GIT					1582:1584	the simulated GIT	1568:1584	the simulated GIT	1568:1584	Raman spectroscopy and scanning electron microscopy showed that there were changes in the composition and structure of the beads throughout the simulated GIT.
33233241	0	73	theme	κ-carrageenan/protein	62:82	arg1	beads					84:88	κ-carrageenan/protein beads	62:88	κ-carrageenan/protein beads	62:88	Digestion of animal- and plant-based proteins encapsulated in κ-carrageenan/protein beads under simulated gastrointestinal conditions.
33233241	8	74	theme	scanning	1451:1458	arg1	microscopy					1469:1478	scanning electron microscopy	1451:1478	scanning electron microscopy	1451:1478	Raman spectroscopy and scanning electron microscopy showed that there were changes in the composition and structure of the beads throughout the simulated GIT.
33233241	7	75	theme	whey	1255:1258	arg1	proteins					1260:1267	the whey proteins	1251:1267	the whey proteins in the stomach phase (p < 0.05)	1251:1299	The soy and pea proteins were digested slightly faster than the whey proteins in the stomach phase (p < 0.05), whereas the pea proteins were digested slightly faster than the soy and whey proteins under intestinal conditions (p < 0.05).
33233241	1	76	theme	polysaccharide/protein	241:262	arg1	beads					264:268	polysaccharide/protein beads	241:268	polysaccharide/protein beads	241:268	In this study, we encapsulated both animal-derived (whey) and plant-derived (soy and pea) proteins within polysaccharide/protein beads and measured the digestion of these beads under simulated gastrointestinal tract (GIT) conditions.
32093025	8	0	theme	BC-ALG	1142:1147	arg1	carrier					1159:1165	the BC-ALG composite carrier	1138:1165	the BC-ALG composite carrier	1138:1165	On the basis of the above results, the BC-ALG composite carrier can be considered ideal for immobilisation of cells for the production of GDL on an industrial scale, and has the potential to be utilised in other biological processes.
32093025	8	1	theme	biological	1315:1324	arg1	processes					1326:1334	other biological processes	1309:1334	other biological processes	1309:1334	On the basis of the above results, the BC-ALG composite carrier can be considered ideal for immobilisation of cells for the production of GDL on an industrial scale, and has the potential to be utilised in other biological processes.
32093025	8	2	theme	above	1123:1127	arg1	results					1129:1135	the above results	1119:1135	the above results	1119:1135	On the basis of the above results, the BC-ALG composite carrier can be considered ideal for immobilisation of cells for the production of GDL on an industrial scale, and has the potential to be utilised in other biological processes.
32093025	0	3	theme	Lactone	86:92	arg1	Production					94:103	Lactone Production	86:103	Lactone Production	86:103	Bacterial Cellulose-Alginate Composite Beads as Yarrowia lipolytica Cell Carriers for Lactone Production.
32093025	4	4	theme	related	511:517	arg1	mechanisms					519:528	related mechanisms	511:528	related mechanisms	511:528	The effects of this carrier on biotransformation and related mechanisms were investigated.
32093025	8	5	contain	has	1273:1275	arg1	carrier					1159:1165	the BC-ALG composite carrier	1138:1165	the BC-ALG composite carrier	1138:1165	On the basis of the above results, the BC-ALG composite carrier can be considered ideal for immobilisation of cells for the production of GDL on an industrial scale, and has the potential to be utilised in other biological processes.
32093025	8	5	contain	has	1273:1275	arg2	potential					1281:1289	the potential to be utilised in other biological processes	1277:1334	the potential to be utilised in other biological processes	1277:1334	On the basis of the above results, the BC-ALG composite carrier can be considered ideal for immobilisation of cells for the production of GDL on an industrial scale, and has the potential to be utilised in other biological processes.
32093025	8	6	theme	other	1309:1313	arg1	processes					1326:1334	other biological processes	1309:1334	other biological processes	1309:1334	On the basis of the above results, the BC-ALG composite carrier can be considered ideal for immobilisation of cells for the production of GDL on an industrial scale, and has the potential to be utilised in other biological processes.
32093025	7	7	theme	multiple	961:968	arg1	interactions					987:998	multiple hydrogen bonding interactions	961:998	multiple hydrogen bonding interactions at the interface between BC and ALG	961:1034	The result indicated that multiple hydrogen bonding interactions at the interface between BC and ALG contributed to the compatibility and stability of BC-ALG carriers.
32093025	3	8	theme	bacterial	355:363	arg1	BC-ALG					385:390	BC-ALG	385:390	BC-ALG	385:390	In this study, a novel composite porous cell carrier, bacterial cellulose-alginate (BC-ALG), was used for long-term biotransformation and production of GDL.
32093025	3	8	theme	bacterial	355:363	arg1	carrier					346:352	a novel composite porous cell carrier	316:352	a novel composite porous cell carrier	316:352	In this study, a novel composite porous cell carrier, bacterial cellulose-alginate (BC-ALG), was used for long-term biotransformation and production of GDL.
32093025	3	8	theme	bacterial	355:363	arg1	cellulose-alginate					365:382	bacterial cellulose-alginate	355:382	bacterial cellulose-alginate (BC-ALG)	355:391	In this study, a novel composite porous cell carrier, bacterial cellulose-alginate (BC-ALG), was used for long-term biotransformation and production of GDL.
32093025	6	9	theme	ALG	916:918	arg1	carrier					920:926	an ALG carrier	913:926	an ALG carrier alone	913:932	In five repeated-batch biotransformation experiments, the maximum concentration of GDL obtained in culture with BC-ALG carriers was 8.37 g/L, approximately 3.7 times higher than that from the medium with an ALG carrier alone.
32093025	5	10	theme	interconnected	676:689	arg1	structure					698:706	an interconnected porous structure	673:706	an interconnected porous structure	673:706	BC-ALG carriers showed improved mechanical strength over ALG carriers, with their internal embedded cell pattern changed to an interconnected porous structure.
32093025	7	11	theme	hydrogen	970:977	arg1	interactions					987:998	multiple hydrogen bonding interactions	961:998	multiple hydrogen bonding interactions at the interface between BC and ALG	961:1034	The result indicated that multiple hydrogen bonding interactions at the interface between BC and ALG contributed to the compatibility and stability of BC-ALG carriers.
32093025	3	12	theme	cell	341:344	arg1	carrier					346:352	a novel composite porous cell carrier	316:352	a novel composite porous cell carrier	316:352	In this study, a novel composite porous cell carrier, bacterial cellulose-alginate (BC-ALG), was used for long-term biotransformation and production of GDL.
32093025	3	12	theme	cell	341:344	arg1	cellulose-alginate					365:382	bacterial cellulose-alginate	355:382	bacterial cellulose-alginate (BC-ALG)	355:391	In this study, a novel composite porous cell carrier, bacterial cellulose-alginate (BC-ALG), was used for long-term biotransformation and production of GDL.
32093025	8	13	from	production	1227:1236	arg1	scale					1262:1266	an industrial scale	1248:1266	an industrial scale	1248:1266	On the basis of the above results, the BC-ALG composite carrier can be considered ideal for immobilisation of cells for the production of GDL on an industrial scale, and has the potential to be utilised in other biological processes.
32093025	5	14	theme	internal	631:638	arg1	pattern					654:660	their internal embedded cell pattern	625:660	their internal embedded cell pattern changed to an interconnected porous structure	625:706	BC-ALG carriers showed improved mechanical strength over ALG carriers, with their internal embedded cell pattern changed to an interconnected porous structure.
32093025	7	15	theme	bonding	979:985	arg1	interactions					987:998	multiple hydrogen bonding interactions	961:998	multiple hydrogen bonding interactions at the interface between BC and ALG	961:1034	The result indicated that multiple hydrogen bonding interactions at the interface between BC and ALG contributed to the compatibility and stability of BC-ALG carriers.
32093025	2	16	theme	industrial	278:287	arg1	production					289:298	its industrial production	274:298	its industrial production	274:298	However, low productivity during bioprocessing limits its industrial production.
32093025	6	17	theme	biotransformation	732:748	arg1	experiments					750:760	five repeated-batch biotransformation experiments	712:760	five repeated-batch biotransformation experiments	712:760	In five repeated-batch biotransformation experiments, the maximum concentration of GDL obtained in culture with BC-ALG carriers was 8.37 g/L, approximately 3.7 times higher than that from the medium with an ALG carrier alone.
32093025	4	18	theme	carrier	478:484	arg1	effects					462:468	The effects	458:468	The effects of this carrier on biotransformation and related mechanisms	458:528	The effects of this carrier on biotransformation and related mechanisms were investigated.
32093025	5	19	theme	embedded	640:647	arg1	pattern					654:660	their internal embedded cell pattern	625:660	their internal embedded cell pattern changed to an interconnected porous structure	625:706	BC-ALG carriers showed improved mechanical strength over ALG carriers, with their internal embedded cell pattern changed to an interconnected porous structure.
32093025	5	20	theme	mechanical	581:590	arg1	strength					592:599	improved mechanical strength	572:599	improved mechanical strength	572:599	BC-ALG carriers showed improved mechanical strength over ALG carriers, with their internal embedded cell pattern changed to an interconnected porous structure.
32093025	8	21	dep	results	1129:1135	arg1	basis					1110:1114	basis	1110:1114	basis	1110:1114	On the basis of the above results, the BC-ALG composite carrier can be considered ideal for immobilisation of cells for the production of GDL on an industrial scale, and has the potential to be utilised in other biological processes.
32093025	8	21	dep	results	1129:1135	arg1	the					1106:1108	the	1106:1108	the	1106:1108	On the basis of the above results, the BC-ALG composite carrier can be considered ideal for immobilisation of cells for the production of GDL on an industrial scale, and has the potential to be utilised in other biological processes.
32093025	6	22	theme	repeated-batch	717:730	arg1	experiments					750:760	five repeated-batch biotransformation experiments	712:760	five repeated-batch biotransformation experiments	712:760	In five repeated-batch biotransformation experiments, the maximum concentration of GDL obtained in culture with BC-ALG carriers was 8.37 g/L, approximately 3.7 times higher than that from the medium with an ALG carrier alone.
32093025	7	23	from	interface	1007:1015	arg1	interactions					987:998	multiple hydrogen bonding interactions	961:998	multiple hydrogen bonding interactions at the interface between BC and ALG	961:1034	The result indicated that multiple hydrogen bonding interactions at the interface between BC and ALG contributed to the compatibility and stability of BC-ALG carriers.
32093025	0	24	theme	Cellulose-Alginate	10:27	arg1	Beads					39:43	Bacterial Cellulose-Alginate Composite Beads	0:43	Bacterial Cellulose-Alginate Composite Beads	0:43	Bacterial Cellulose-Alginate Composite Beads as Yarrowia lipolytica Cell Carriers for Lactone Production.
32093025	3	25	theme	novel	318:322	arg1	carrier					346:352	a novel composite porous cell carrier	316:352	a novel composite porous cell carrier	316:352	In this study, a novel composite porous cell carrier, bacterial cellulose-alginate (BC-ALG), was used for long-term biotransformation and production of GDL.
32093025	3	25	theme	novel	318:322	arg1	cellulose-alginate					365:382	bacterial cellulose-alginate	355:382	bacterial cellulose-alginate (BC-ALG)	355:391	In this study, a novel composite porous cell carrier, bacterial cellulose-alginate (BC-ALG), was used for long-term biotransformation and production of GDL.
32093025	5	26	theme	cell	649:652	arg1	pattern					654:660	their internal embedded cell pattern	625:660	their internal embedded cell pattern changed to an interconnected porous structure	625:706	BC-ALG carriers showed improved mechanical strength over ALG carriers, with their internal embedded cell pattern changed to an interconnected porous structure.
32093025	6	27	theme	BC-ALG	821:826	arg1	carriers					828:835	BC-ALG carriers	821:835	BC-ALG carriers	821:835	In five repeated-batch biotransformation experiments, the maximum concentration of GDL obtained in culture with BC-ALG carriers was 8.37 g/L, approximately 3.7 times higher than that from the medium with an ALG carrier alone.
32093025	3	28	theme	long-term	407:415	arg1	biotransformation					417:433	long-term biotransformation	407:433	long-term biotransformation	407:433	In this study, a novel composite porous cell carrier, bacterial cellulose-alginate (BC-ALG), was used for long-term biotransformation and production of GDL.
32093025	0	29	theme	Bacterial	0:8	arg1	Beads					39:43	Bacterial Cellulose-Alginate Composite Beads	0:43	Bacterial Cellulose-Alginate Composite Beads	0:43	Bacterial Cellulose-Alginate Composite Beads as Yarrowia lipolytica Cell Carriers for Lactone Production.
32093025	4	30	from	effects	462:468	arg1	biotransformation					489:505	biotransformation	489:505	biotransformation	489:505	The effects of this carrier on biotransformation and related mechanisms were investigated.
32093025	4	30	from	effects	462:468	arg1	mechanisms					519:528	related mechanisms	511:528	related mechanisms	511:528	The effects of this carrier on biotransformation and related mechanisms were investigated.
32093025	6	31	from	g/L	846:848	arg1	experiments					750:760	five repeated-batch biotransformation experiments	712:760	five repeated-batch biotransformation experiments	712:760	In five repeated-batch biotransformation experiments, the maximum concentration of GDL obtained in culture with BC-ALG carriers was 8.37 g/L, approximately 3.7 times higher than that from the medium with an ALG carrier alone.
32093025	8	32	theme	cells	1213:1217	arg1	immobilisation					1195:1208	immobilisation	1195:1208	immobilisation of cells	1195:1217	On the basis of the above results, the BC-ALG composite carrier can be considered ideal for immobilisation of cells for the production of GDL on an industrial scale, and has the potential to be utilised in other biological processes.
32093025	7	33	theme	carriers	1093:1100	arg1	stability					1073:1081	stability	1073:1081	stability	1073:1081	The result indicated that multiple hydrogen bonding interactions at the interface between BC and ALG contributed to the compatibility and stability of BC-ALG carriers.
32093025	7	33	theme	carriers	1093:1100	arg1	compatibility					1055:1067	compatibility	1055:1067	compatibility	1055:1067	The result indicated that multiple hydrogen bonding interactions at the interface between BC and ALG contributed to the compatibility and stability of BC-ALG carriers.
32093025	5	34	theme	ALG	606:608	arg1	carriers					610:617	ALG carriers	606:617	ALG carriers	606:617	BC-ALG carriers showed improved mechanical strength over ALG carriers, with their internal embedded cell pattern changed to an interconnected porous structure.
32093025	1	35	theme	food	192:195	arg1	fields					182:187	the fields	178:187	the fields of food and cosmetic products	178:217	The demand for natural lactone gamma-decalactone (GDL) has increased in the fields of food and cosmetic products.
32093025	0	36	theme	Composite	29:37	arg1	Beads					39:43	Bacterial Cellulose-Alginate Composite Beads	0:43	Bacterial Cellulose-Alginate Composite Beads	0:43	Bacterial Cellulose-Alginate Composite Beads as Yarrowia lipolytica Cell Carriers for Lactone Production.
32093025	6	37	dep	times	869:873	arg1	higher					875:880	higher	875:880	higher	875:880	In five repeated-batch biotransformation experiments, the maximum concentration of GDL obtained in culture with BC-ALG carriers was 8.37 g/L, approximately 3.7 times higher than that from the medium with an ALG carrier alone.
32093025	2	38	theme	low	229:231	arg1	productivity					233:244	low productivity	229:244	low productivity during bioprocessing	229:265	However, low productivity during bioprocessing limits its industrial production.
32093025	1	39	theme	cosmetic	201:208	arg1	products					210:217	cosmetic products	201:217	cosmetic products	201:217	The demand for natural lactone gamma-decalactone (GDL) has increased in the fields of food and cosmetic products.
32093025	6	40	with	culture	808:814	arg1	carriers					828:835	BC-ALG carriers	821:835	BC-ALG carriers	821:835	In five repeated-batch biotransformation experiments, the maximum concentration of GDL obtained in culture with BC-ALG carriers was 8.37 g/L, approximately 3.7 times higher than that from the medium with an ALG carrier alone.
32093025	7	41	theme	BC-ALG	1086:1091	arg1	carriers					1093:1100	BC-ALG carriers	1086:1100	BC-ALG carriers	1086:1100	The result indicated that multiple hydrogen bonding interactions at the interface between BC and ALG contributed to the compatibility and stability of BC-ALG carriers.
32093025	8	42	theme	composite	1149:1157	arg1	carrier					1159:1165	the BC-ALG composite carrier	1138:1165	the BC-ALG composite carrier	1138:1165	On the basis of the above results, the BC-ALG composite carrier can be considered ideal for immobilisation of cells for the production of GDL on an industrial scale, and has the potential to be utilised in other biological processes.
32093025	5	43	theme	BC-ALG	549:554	arg1	carriers					556:563	BC-ALG carriers	549:563	BC-ALG carriers	549:563	BC-ALG carriers showed improved mechanical strength over ALG carriers, with their internal embedded cell pattern changed to an interconnected porous structure.
32093025	3	44	theme	GDL	453:455	arg1	production					439:448	production	439:448	production of GDL	439:455	In this study, a novel composite porous cell carrier, bacterial cellulose-alginate (BC-ALG), was used for long-term biotransformation and production of GDL.
32093025	3	44	theme	GDL	453:455	arg1	biotransformation					417:433	long-term biotransformation	407:433	long-term biotransformation	407:433	In this study, a novel composite porous cell carrier, bacterial cellulose-alginate (BC-ALG), was used for long-term biotransformation and production of GDL.
32093025	0	45	theme	Cell	68:71	arg1	Carriers					73:80	Yarrowia lipolytica Cell Carriers	48:80	Yarrowia lipolytica Cell Carriers for Lactone Production	48:103	Bacterial Cellulose-Alginate Composite Beads as Yarrowia lipolytica Cell Carriers for Lactone Production.
32093025	5	46	theme	porous	691:696	arg1	structure					698:706	an interconnected porous structure	673:706	an interconnected porous structure	673:706	BC-ALG carriers showed improved mechanical strength over ALG carriers, with their internal embedded cell pattern changed to an interconnected porous structure.
32093025	1	47	theme	natural	121:127	arg1	gamma-decalactone					137:153	natural lactone gamma-decalactone	121:153	natural lactone gamma-decalactone (GDL)	121:159	The demand for natural lactone gamma-decalactone (GDL) has increased in the fields of food and cosmetic products.
32093025	1	47	theme	natural	121:127	arg1	GDL					156:158	GDL	156:158	GDL	156:158	The demand for natural lactone gamma-decalactone (GDL) has increased in the fields of food and cosmetic products.
32093025	0	48	theme	lipolytica	57:66	arg1	Carriers					73:80	Yarrowia lipolytica Cell Carriers	48:80	Yarrowia lipolytica Cell Carriers for Lactone Production	48:103	Bacterial Cellulose-Alginate Composite Beads as Yarrowia lipolytica Cell Carriers for Lactone Production.
32093025	8	49	theme	industrial	1251:1260	arg1	scale					1262:1266	an industrial scale	1248:1266	an industrial scale	1248:1266	On the basis of the above results, the BC-ALG composite carrier can be considered ideal for immobilisation of cells for the production of GDL on an industrial scale, and has the potential to be utilised in other biological processes.
32093025	6	50	theme	GDL	792:794	arg1	concentration					775:787	the maximum concentration	763:787	the maximum concentration of GDL obtained in culture with BC-ALG carriers	763:835	In five repeated-batch biotransformation experiments, the maximum concentration of GDL obtained in culture with BC-ALG carriers was 8.37 g/L, approximately 3.7 times higher than that from the medium with an ALG carrier alone.
32093025	6	50	theme	GDL	792:794	arg1	g/L					846:848	8.37 g/L	841:848	8.37 g/L	841:848	In five repeated-batch biotransformation experiments, the maximum concentration of GDL obtained in culture with BC-ALG carriers was 8.37 g/L, approximately 3.7 times higher than that from the medium with an ALG carrier alone.
32093025	1	51	theme	lactone	129:135	arg1	gamma-decalactone					137:153	natural lactone gamma-decalactone	121:153	natural lactone gamma-decalactone (GDL)	121:159	The demand for natural lactone gamma-decalactone (GDL) has increased in the fields of food and cosmetic products.
32093025	1	51	theme	lactone	129:135	arg1	GDL					156:158	GDL	156:158	GDL	156:158	The demand for natural lactone gamma-decalactone (GDL) has increased in the fields of food and cosmetic products.
32093025	0	52	dep	Beads	39:43	arg1	Carriers					73:80	Yarrowia lipolytica Cell Carriers	48:80	Yarrowia lipolytica Cell Carriers for Lactone Production	48:103	Bacterial Cellulose-Alginate Composite Beads as Yarrowia lipolytica Cell Carriers for Lactone Production.
32093025	5	53	theme	improved	572:579	arg1	strength					592:599	improved mechanical strength	572:599	improved mechanical strength	572:599	BC-ALG carriers showed improved mechanical strength over ALG carriers, with their internal embedded cell pattern changed to an interconnected porous structure.
32093025	3	54	used	used	398:401	arg2	cellulose-alginate					365:382	bacterial cellulose-alginate	355:382	bacterial cellulose-alginate (BC-ALG)	355:391	In this study, a novel composite porous cell carrier, bacterial cellulose-alginate (BC-ALG), was used for long-term biotransformation and production of GDL.
32093025	3	54	used	used	398:401	arg2	carrier					346:352	a novel composite porous cell carrier	316:352	a novel composite porous cell carrier	316:352	In this study, a novel composite porous cell carrier, bacterial cellulose-alginate (BC-ALG), was used for long-term biotransformation and production of GDL.
32093025	6	55	theme	3.7	865:867	arg1	times					869:873	times	869:873	times	869:873	In five repeated-batch biotransformation experiments, the maximum concentration of GDL obtained in culture with BC-ALG carriers was 8.37 g/L, approximately 3.7 times higher than that from the medium with an ALG carrier alone.
32093025	6	55	theme	3.7	865:867	arg1	g/L					846:848	8.37 g/L	841:848	8.37 g/L	841:848	In five repeated-batch biotransformation experiments, the maximum concentration of GDL obtained in culture with BC-ALG carriers was 8.37 g/L, approximately 3.7 times higher than that from the medium with an ALG carrier alone.
32093025	3	56	theme	composite	324:332	arg1	carrier					346:352	a novel composite porous cell carrier	316:352	a novel composite porous cell carrier	316:352	In this study, a novel composite porous cell carrier, bacterial cellulose-alginate (BC-ALG), was used for long-term biotransformation and production of GDL.
32093025	3	56	theme	composite	324:332	arg1	cellulose-alginate					365:382	bacterial cellulose-alginate	355:382	bacterial cellulose-alginate (BC-ALG)	355:391	In this study, a novel composite porous cell carrier, bacterial cellulose-alginate (BC-ALG), was used for long-term biotransformation and production of GDL.
32093025	1	57	theme	products	210:217	arg1	fields					182:187	the fields	178:187	the fields of food and cosmetic products	178:217	The demand for natural lactone gamma-decalactone (GDL) has increased in the fields of food and cosmetic products.
32093025	6	58	theme	maximum	767:773	arg1	concentration					775:787	the maximum concentration	763:787	the maximum concentration of GDL obtained in culture with BC-ALG carriers	763:835	In five repeated-batch biotransformation experiments, the maximum concentration of GDL obtained in culture with BC-ALG carriers was 8.37 g/L, approximately 3.7 times higher than that from the medium with an ALG carrier alone.
32093025	6	58	theme	maximum	767:773	arg1	g/L					846:848	8.37 g/L	841:848	8.37 g/L	841:848	In five repeated-batch biotransformation experiments, the maximum concentration of GDL obtained in culture with BC-ALG carriers was 8.37 g/L, approximately 3.7 times higher than that from the medium with an ALG carrier alone.
32093025	8	59	theme	GDL	1241:1243	arg1	production					1227:1236	the production	1223:1236	the production of GDL on an industrial scale	1223:1266	On the basis of the above results, the BC-ALG composite carrier can be considered ideal for immobilisation of cells for the production of GDL on an industrial scale, and has the potential to be utilised in other biological processes.
32093025	3	60	theme	porous	334:339	arg1	carrier					346:352	a novel composite porous cell carrier	316:352	a novel composite porous cell carrier	316:352	In this study, a novel composite porous cell carrier, bacterial cellulose-alginate (BC-ALG), was used for long-term biotransformation and production of GDL.
32093025	3	60	theme	porous	334:339	arg1	cellulose-alginate					365:382	bacterial cellulose-alginate	355:382	bacterial cellulose-alginate (BC-ALG)	355:391	In this study, a novel composite porous cell carrier, bacterial cellulose-alginate (BC-ALG), was used for long-term biotransformation and production of GDL.
32093025	6	61	with	medium	901:906	arg1	carrier					920:926	an ALG carrier	913:926	an ALG carrier alone	913:932	In five repeated-batch biotransformation experiments, the maximum concentration of GDL obtained in culture with BC-ALG carriers was 8.37 g/L, approximately 3.7 times higher than that from the medium with an ALG carrier alone.
32093025	7	62	dep	compatibility	1055:1067	arg1	the					1051:1053	the	1051:1053	the	1051:1053	The result indicated that multiple hydrogen bonding interactions at the interface between BC and ALG contributed to the compatibility and stability of BC-ALG carriers.
34070741	4	0	theme	severe	890:895	arg1	maldevelopment					897:910	severe maldevelopment	890:910	severe maldevelopment with incomplete axonal branching	890:943	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	5	1	contain	had	1044:1046	arg2	activity					1076:1083	impaired swimming locomotor activity	1048:1083	impaired swimming locomotor activity	1048:1083	Consequently, larvae expressing N220Q in CaP neurons had impaired swimming locomotor activity.
34070741	5	1	contain	had	1044:1046	arg1	larvae					1005:1010	larvae	1005:1010	larvae expressing N220Q in CaP neurons	1005:1042	Consequently, larvae expressing N220Q in CaP neurons had impaired swimming locomotor activity.
34070741	6	2	theme	cells	1338:1342	arg1	membranes					1307:1315	membranes	1307:1315	membranes of rat neuroblastoma cells	1307:1342	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	6	3	from	particles	1294:1302	arg1	membranes					1307:1315	membranes	1307:1315	membranes of rat neuroblastoma cells	1307:1342	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	7	4	theme	Kv3.1b	1527:1532	arg1	α-subunit					1534:1542	the Kv3.1b α-subunit	1523:1542	the Kv3.1b α-subunit	1523:1542	Opening and closing rates were slowed in Kv3 channels containing Kv3.1b with oligomannose, instead of complex N-glycans, which suggested a reduction in the intrinsic dynamics of the Kv3.1b α-subunit.
34070741	6	5	theme	rat	1320:1322	arg1	cells					1338:1342	rat neuroblastoma cells	1320:1342	rat neuroblastoma cells	1320:1342	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	3	6	theme	engineered	573:582	arg1	cells					587:591	engineered NB cells	573:591	engineered NB cells	573:591	Here we examined neuron development, localization, and activity of Kv3 channels in wildtype AB zebrafish and CRISPR/Cas9 engineered NB cells, due to perturbations in N-glycosylation processing of Kv3.1b.
34070741	6	7	from	membranes	1307:1315	arg1	size					1255:1258	size	1255:1258	size	1255:1258	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	6	7	from	membranes	1307:1315	arg1	density					1265:1271	density	1265:1271	density	1265:1271	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	6	7	from	membranes	1307:1315	arg1	number					1247:1252	number	1247:1252	number	1247:1252	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	2	8	dep	sites	311:315	arg1	sites					311:315	Vacant N-glycosylation sites	288:315	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons	288:388	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	2	8	dep	sites	311:315	arg1	channels					358:365	Kv3, voltage-gated K+ channels	336:365	channels	358:365	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	2	8	dep	sites	311:315	arg1	N229					327:330	N229	327:330	N229	327:330	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	2	8	dep	sites	311:315	arg1	N220					318:321	N220	318:321	N220	318:321	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	1	9	theme	glycosylation	218:230	arg1	disorders					205:213	congenital disorders	194:213	congenital disorders of glycosylation	194:230	Neurological difficulties commonly accompany individuals suffering from congenital disorders of glycosylation, resulting from defects in the N-glycosylation pathway.
34070741	7	10	theme	intrinsic	1501:1509	arg1	dynamics					1511:1518	the intrinsic dynamics	1497:1518	the intrinsic dynamics of the Kv3.1b α-subunit	1497:1542	Opening and closing rates were slowed in Kv3 channels containing Kv3.1b with oligomannose, instead of complex N-glycans, which suggested a reduction in the intrinsic dynamics of the Kv3.1b α-subunit.
34070741	6	11	theme	particles	1294:1302	arg1	size					1255:1258	size	1255:1258	size	1255:1258	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	6	11	theme	particles	1294:1302	arg1	density					1265:1271	density	1265:1271	density	1265:1271	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	6	11	theme	particles	1294:1302	arg1	number					1247:1252	number	1247:1252	number	1247:1252	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	2	12	theme	voltage-gated	341:353	arg1	channels					358:365	Kv3, voltage-gated K+ channels	336:365	channels	358:365	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	6	13	theme	Kv3.1b	1200:1205	arg1	dispersal					1207:1215	Kv3.1b dispersal	1200:1215	Kv3.1b dispersal	1200:1215	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	2	14	theme	Kv3	336:338	arg1	channels					358:365	Kv3, voltage-gated K+ channels	336:365	channels	358:365	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	6	15	dep	number	1247:1252	arg1	the					1243:1245	the	1243:1245	the	1243:1245	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	4	16	theme	ventral	970:976	arg1	musculature					978:988	the ventral musculature	966:988	the ventral musculature	966:988	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	6	17	theme	Kv3.1b-containing	1276:1292	arg1	particles					1294:1302	Kv3.1b-containing particles	1276:1302	Kv3.1b-containing particles in membranes of rat neuroblastoma cells	1276:1342	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	4	18	theme	caudal	671:676	arg1	neurons					698:704	caudal primary (CaP) motor neurons	671:704	caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b	671:783	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	4	19	contain	have	785:788	arg2	morphology					804:813	stereotypical morphology	790:813	stereotypical morphology	790:813	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	4	19	contain	have	785:788	arg1	neurons					698:704	caudal primary (CaP) motor neurons	671:704	caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b	671:783	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	3	20	theme	N-glycosylation	618:632	arg1	processing					634:643	N-glycosylation processing	618:643	N-glycosylation processing of Kv3.1b	618:653	Here we examined neuron development, localization, and activity of Kv3 channels in wildtype AB zebrafish and CRISPR/Cas9 engineered NB cells, due to perturbations in N-glycosylation processing of Kv3.1b.
34070741	4	21	theme	axonal	928:933	arg1	branching					935:943	incomplete axonal branching	917:943	incomplete axonal branching	917:943	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	4	22	theme	cord	726:729	arg1	neurons					698:704	caudal primary (CaP) motor neurons	671:704	caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b	671:783	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	0	23	theme	Motor	75:79	arg1	Structure					88:96	Perturbed Motor Neuron Structure	65:96	Perturbed Motor Neuron Structure	65:96	Compromised N-Glycosylation Processing of Kv3.1b Correlates with Perturbed Motor Neuron Structure and Locomotor Activity.
34070741	2	24	theme	N-glycosylation	295:309	arg1	sites					311:315	Vacant N-glycosylation sites	288:315	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons	288:388	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	2	24	theme	N-glycosylation	295:309	arg1	channels					358:365	Kv3, voltage-gated K+ channels	336:365	channels	358:365	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	2	24	theme	N-glycosylation	295:309	arg1	N229					327:330	N229	327:330	N229	327:330	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	2	24	theme	N-glycosylation	295:309	arg1	N220					318:321	N220	318:321	N220	318:321	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	4	25	theme	zebrafish	709:717	arg1	cord					726:729	zebrafish spinal cord	709:729	zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b	709:783	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	6	26	from	number	1247:1252	arg1	membranes					1307:1315	membranes	1307:1315	membranes of rat neuroblastoma cells	1307:1342	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	7	27	from	reduction	1484:1492	arg1	dynamics					1511:1518	the intrinsic dynamics	1497:1518	the intrinsic dynamics of the Kv3.1b α-subunit	1497:1542	Opening and closing rates were slowed in Kv3 channels containing Kv3.1b with oligomannose, instead of complex N-glycans, which suggested a reduction in the intrinsic dynamics of the Kv3.1b α-subunit.
34070741	8	28	theme	N-glycosylation	1551:1565	arg1	processing					1567:1576	N-glycosylation processing	1551:1576	N-glycosylation processing of Kv3.1b	1551:1586	Thus, N-glycosylation processing of Kv3.1b regulates neuronal development and excitability, thereby controlling motor activity.
34070741	6	29	from	size	1255:1258	arg1	membranes					1307:1315	membranes	1307:1315	membranes of rat neuroblastoma cells	1307:1342	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	0	30	theme	Locomotor	102:110	arg1	Activity					112:119	Locomotor Activity	102:119	Locomotor Activity	102:119	Compromised N-Glycosylation Processing of Kv3.1b Correlates with Perturbed Motor Neuron Structure and Locomotor Activity.
34070741	4	31	theme	stereotypical	790:802	arg1	morphology					804:813	stereotypical morphology	790:813	stereotypical morphology	790:813	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	6	32	theme	N-glycans	1124:1132	arg1	replacement					1101:1111	replacement	1101:1111	replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface	1101:1189	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	4	33	theme	glycosylated	760:771	arg1	Kv3.1b					778:783	fully glycosylated (WT) Kv3.1b	754:783	fully glycosylated (WT) Kv3.1b	754:783	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	0	34	theme	Compromised	0:10	arg1	Processing					28:37	Compromised N-Glycosylation Processing	0:37	Compromised N-Glycosylation Processing of Kv3.1b	0:47	Compromised N-Glycosylation Processing of Kv3.1b Correlates with Perturbed Motor Neuron Structure and Locomotor Activity.
34070741	3	35	from	perturbations	601:613	arg1	processing					634:643	N-glycosylation processing	618:643	N-glycosylation processing of Kv3.1b	618:653	Here we examined neuron development, localization, and activity of Kv3 channels in wildtype AB zebrafish and CRISPR/Cas9 engineered NB cells, due to perturbations in N-glycosylation processing of Kv3.1b.
34070741	4	36	gly	glycosylated	855:866	arg1	Kv3.1b					876:881	partially glycosylated (N220Q) Kv3.1b	845:881	partially glycosylated (N220Q) Kv3.1b	845:881	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	1	37	theme	Neurological	122:133	arg1	difficulties					135:146	Neurological difficulties	122:146	Neurological difficulties	122:146	Neurological difficulties commonly accompany individuals suffering from congenital disorders of glycosylation, resulting from defects in the N-glycosylation pathway.
34070741	0	38	theme	Kv3.1b	42:47	arg1	Processing					28:37	Compromised N-Glycosylation Processing	0:37	Compromised N-Glycosylation Processing of Kv3.1b	0:47	Compromised N-Glycosylation Processing of Kv3.1b Correlates with Perturbed Motor Neuron Structure and Locomotor Activity.
34070741	7	39	theme	complex	1447:1453	arg1	N-glycans					1455:1463	complex N-glycans	1447:1463	complex N-glycans	1447:1463	Opening and closing rates were slowed in Kv3 channels containing Kv3.1b with oligomannose, instead of complex N-glycans, which suggested a reduction in the intrinsic dynamics of the Kv3.1b α-subunit.
34070741	5	40	theme	locomotor	1066:1074	arg1	activity					1076:1083	impaired swimming locomotor activity	1048:1083	impaired swimming locomotor activity	1048:1083	Consequently, larvae expressing N220Q in CaP neurons had impaired swimming locomotor activity.
34070741	6	41	attach	attached	1152:1159	arg2	oligomannose					1139:1150	oligomannose	1139:1150	oligomannose attached to Kv3.1b	1139:1169	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	6	41	attach	attached	1152:1159	arg1	Kv3.1b					1164:1169	Kv3.1b	1164:1169	Kv3.1b	1164:1169	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	4	42	theme	glycosylated	855:866	arg1	Kv3.1b					876:881	partially glycosylated (N220Q) Kv3.1b	845:881	partially glycosylated (N220Q) Kv3.1b	845:881	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	2	43	theme	neurons	382:388	arg1	channels					358:365	Kv3, voltage-gated K+ channels	336:365	channels	358:365	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	7	44	theme	Kv3	1386:1388	arg1	channels					1390:1397	Kv3 channels	1386:1397	Kv3 channels containing Kv3.1b with oligomannose, instead of complex N-glycans, which suggested a reduction in the intrinsic dynamics of the Kv3.1b α-subunit	1386:1542	Opening and closing rates were slowed in Kv3 channels containing Kv3.1b with oligomannose, instead of complex N-glycans, which suggested a reduction in the intrinsic dynamics of the Kv3.1b α-subunit.
34070741	6	45	theme	cell	1178:1181	arg1	surface					1183:1189	cell surface	1178:1189	cell surface	1178:1189	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	8	46	theme	motor	1657:1661	arg1	activity					1663:1670	motor activity	1657:1670	motor activity	1657:1670	Thus, N-glycosylation processing of Kv3.1b regulates neuronal development and excitability, thereby controlling motor activity.
34070741	7	47	contain	containing	1399:1408	arg1	channels					1390:1397	Kv3 channels	1386:1397	Kv3 channels containing Kv3.1b with oligomannose, instead of complex N-glycans, which suggested a reduction in the intrinsic dynamics of the Kv3.1b α-subunit	1386:1542	Opening and closing rates were slowed in Kv3 channels containing Kv3.1b with oligomannose, instead of complex N-glycans, which suggested a reduction in the intrinsic dynamics of the Kv3.1b α-subunit.
34070741	7	47	contain	containing	1399:1408	arg2	Kv3.1b					1410:1415	Kv3.1b	1410:1415	Kv3.1b	1410:1415	Opening and closing rates were slowed in Kv3 channels containing Kv3.1b with oligomannose, instead of complex N-glycans, which suggested a reduction in the intrinsic dynamics of the Kv3.1b α-subunit.
34070741	6	48	with	replacement	1101:1111	arg1	oligomannose					1139:1150	oligomannose	1139:1150	oligomannose attached to Kv3.1b	1139:1169	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	4	49	theme	CaP	822:824	arg1	neurons					826:832	CaP neurons	822:832	CaP neurons expressing partially glycosylated (N220Q) Kv3.1b	822:881	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	3	50	theme	wildtype	535:542	arg1	zebrafish					547:555	wildtype AB zebrafish	535:555	wildtype AB zebrafish	535:555	Here we examined neuron development, localization, and activity of Kv3 channels in wildtype AB zebrafish and CRISPR/Cas9 engineered NB cells, due to perturbations in N-glycosylation processing of Kv3.1b.
34070741	7	51	theme	α-subunit	1534:1542	arg1	dynamics					1511:1518	the intrinsic dynamics	1497:1518	the intrinsic dynamics of the Kv3.1b α-subunit	1497:1542	Opening and closing rates were slowed in Kv3 channels containing Kv3.1b with oligomannose, instead of complex N-glycans, which suggested a reduction in the intrinsic dynamics of the Kv3.1b α-subunit.
34070741	5	52	theme	impaired	1048:1055	arg1	activity					1076:1083	impaired swimming locomotor activity	1048:1083	impaired swimming locomotor activity	1048:1083	Consequently, larvae expressing N220Q in CaP neurons had impaired swimming locomotor activity.
34070741	3	53	theme	channels	523:530	arg1	localization					489:500	localization	489:500	localization	489:500	Here we examined neuron development, localization, and activity of Kv3 channels in wildtype AB zebrafish and CRISPR/Cas9 engineered NB cells, due to perturbations in N-glycosylation processing of Kv3.1b.
34070741	3	53	theme	channels	523:530	arg1	activity					507:514	activity	507:514	activity	507:514	Here we examined neuron development, localization, and activity of Kv3 channels in wildtype AB zebrafish and CRISPR/Cas9 engineered NB cells, due to perturbations in N-glycosylation processing of Kv3.1b.
34070741	3	53	theme	channels	523:530	arg1	development					476:486	neuron development	469:486	neuron development	469:486	Here we examined neuron development, localization, and activity of Kv3 channels in wildtype AB zebrafish and CRISPR/Cas9 engineered NB cells, due to perturbations in N-glycosylation processing of Kv3.1b.
34070741	6	54	theme	neuroblastoma	1324:1336	arg1	cells					1338:1342	rat neuroblastoma cells	1320:1342	rat neuroblastoma cells	1320:1342	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	4	55	theme	primary	678:684	arg1	neurons					698:704	caudal primary (CaP) motor neurons	671:704	caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b	671:783	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	3	56	from	activity	507:514	arg1	CRISPR/Cas9					561:571	CRISPR/Cas9	561:571	CRISPR/Cas9	561:571	Here we examined neuron development, localization, and activity of Kv3 channels in wildtype AB zebrafish and CRISPR/Cas9 engineered NB cells, due to perturbations in N-glycosylation processing of Kv3.1b.
34070741	3	56	from	activity	507:514	arg1	zebrafish					547:555	wildtype AB zebrafish	535:555	wildtype AB zebrafish	535:555	Here we examined neuron development, localization, and activity of Kv3 channels in wildtype AB zebrafish and CRISPR/Cas9 engineered NB cells, due to perturbations in N-glycosylation processing of Kv3.1b.
34070741	2	57	theme	K+	355:356	arg1	channels					358:365	Kv3, voltage-gated K+ channels	336:365	channels	358:365	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	4	58	theme	CaP	687:689	arg1	neurons					698:704	caudal primary (CaP) motor neurons	671:704	caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b	671:783	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	2	59	gly	N-glycosylation	295:309	arg2	N220					318:321	N220	318:321	N220	318:321	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	2	59	gly	N-glycosylation	295:309	arg2	channels					358:365	Kv3, voltage-gated K+ channels	336:365	channels	358:365	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	2	59	gly	N-glycosylation	295:309	arg2	sites					311:315	Vacant N-glycosylation sites	288:315	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons	288:388	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	2	59	gly	N-glycosylation	295:309	arg1	channels					358:365	Kv3, voltage-gated K+ channels	336:365	channels	358:365	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	2	59	gly	N-glycosylation	295:309	arg2	N229					327:330	N229	327:330	N229	327:330	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	1	60	theme	N-glycosylation	263:277	arg1	pathway					279:285	the N-glycosylation pathway	259:285	the N-glycosylation pathway	259:285	Neurological difficulties commonly accompany individuals suffering from congenital disorders of glycosylation, resulting from defects in the N-glycosylation pathway.
34070741	3	61	theme	NB	584:585	arg1	cells					587:591	engineered NB cells	573:591	engineered NB cells	573:591	Here we examined neuron development, localization, and activity of Kv3 channels in wildtype AB zebrafish and CRISPR/Cas9 engineered NB cells, due to perturbations in N-glycosylation processing of Kv3.1b.
34070741	4	62	dep	glycosylated	855:866	arg1	N220Q					869:873	N220Q	869:873	N220Q	869:873	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	3	63	from	localization	489:500	arg1	CRISPR/Cas9					561:571	CRISPR/Cas9	561:571	CRISPR/Cas9	561:571	Here we examined neuron development, localization, and activity of Kv3 channels in wildtype AB zebrafish and CRISPR/Cas9 engineered NB cells, due to perturbations in N-glycosylation processing of Kv3.1b.
34070741	3	63	from	localization	489:500	arg1	zebrafish					547:555	wildtype AB zebrafish	535:555	wildtype AB zebrafish	535:555	Here we examined neuron development, localization, and activity of Kv3 channels in wildtype AB zebrafish and CRISPR/Cas9 engineered NB cells, due to perturbations in N-glycosylation processing of Kv3.1b.
34070741	7	64	theme	N-glycans	1455:1463	arg1	Kv3.1b					1410:1415	Kv3.1b	1410:1415	Kv3.1b	1410:1415	Opening and closing rates were slowed in Kv3 channels containing Kv3.1b with oligomannose, instead of complex N-glycans, which suggested a reduction in the intrinsic dynamics of the Kv3.1b α-subunit.
34070741	2	65	theme	high-firing	370:380	arg1	neurons					382:388	high-firing neurons	370:388	high-firing neurons	370:388	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	2	66	theme	Vacant	288:293	arg1	sites					311:315	Vacant N-glycosylation sites	288:315	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons	288:388	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	2	66	theme	Vacant	288:293	arg1	channels					358:365	Kv3, voltage-gated K+ channels	336:365	channels	358:365	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	2	66	theme	Vacant	288:293	arg1	N229					327:330	N229	327:330	N229	327:330	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	2	66	theme	Vacant	288:293	arg1	N220					318:321	N220	318:321	N220	318:321	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	3	67	dep	zebrafish	547:555	arg1	cells					587:591	engineered NB cells	573:591	engineered NB cells	573:591	Here we examined neuron development, localization, and activity of Kv3 channels in wildtype AB zebrafish and CRISPR/Cas9 engineered NB cells, due to perturbations in N-glycosylation processing of Kv3.1b.
34070741	4	68	theme	incomplete	917:926	arg1	branching					935:943	incomplete axonal branching	917:943	incomplete axonal branching	917:943	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	4	69	theme	spinal	719:724	arg1	cord					726:729	zebrafish spinal cord	709:729	zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b	709:783	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	6	70	from	density	1265:1271	arg1	membranes					1307:1315	membranes	1307:1315	membranes of rat neuroblastoma cells	1307:1342	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	3	71	theme	Kv3.1b	648:653	arg1	processing					634:643	N-glycosylation processing	618:643	N-glycosylation processing of Kv3.1b	618:653	Here we examined neuron development, localization, and activity of Kv3 channels in wildtype AB zebrafish and CRISPR/Cas9 engineered NB cells, due to perturbations in N-glycosylation processing of Kv3.1b.
34070741	1	72	from	defects	248:254	arg1	pathway					279:285	the N-glycosylation pathway	259:285	the N-glycosylation pathway	259:285	Neurological difficulties commonly accompany individuals suffering from congenital disorders of glycosylation, resulting from defects in the N-glycosylation pathway.
34070741	4	73	dep	glycosylated	760:771	arg1	WT					774:775	WT	774:775	WT	774:775	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	4	74	with	maldevelopment	897:910	arg1	branching					935:943	incomplete axonal branching	917:943	incomplete axonal branching	917:943	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	0	75	theme	Neuron	81:86	arg1	Structure					88:96	Perturbed Motor Neuron Structure	65:96	Perturbed Motor Neuron Structure	65:96	Compromised N-Glycosylation Processing of Kv3.1b Correlates with Perturbed Motor Neuron Structure and Locomotor Activity.
34070741	4	76	theme	motor	692:696	arg1	neurons					698:704	caudal primary (CaP) motor neurons	671:704	caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b	671:783	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	4	77	gly	glycosylated	760:771	arg1	Kv3.1b					778:783	fully glycosylated (WT) Kv3.1b	754:783	fully glycosylated (WT) Kv3.1b	754:783	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	7	78	with	Kv3.1b	1410:1415	arg1	oligomannose					1422:1433	oligomannose	1422:1433	oligomannose	1422:1433	Opening and closing rates were slowed in Kv3 channels containing Kv3.1b with oligomannose, instead of complex N-glycans, which suggested a reduction in the intrinsic dynamics of the Kv3.1b α-subunit.
34070741	1	79	theme	congenital	194:203	arg1	disorders					205:213	congenital disorders	194:213	congenital disorders of glycosylation	194:230	Neurological difficulties commonly accompany individuals suffering from congenital disorders of glycosylation, resulting from defects in the N-glycosylation pathway.
34070741	8	80	theme	Kv3.1b	1581:1586	arg1	processing					1567:1576	N-glycosylation processing	1551:1576	N-glycosylation processing of Kv3.1b	1551:1586	Thus, N-glycosylation processing of Kv3.1b regulates neuronal development and excitability, thereby controlling motor activity.
34070741	8	81	theme	neuronal	1598:1605	arg1	development					1607:1617	neuronal development	1598:1617	neuronal development	1598:1617	Thus, N-glycosylation processing of Kv3.1b regulates neuronal development and excitability, thereby controlling motor activity.
34070741	3	82	theme	neuron	469:474	arg1	development					476:486	neuron development	469:486	neuron development	469:486	Here we examined neuron development, localization, and activity of Kv3 channels in wildtype AB zebrafish and CRISPR/Cas9 engineered NB cells, due to perturbations in N-glycosylation processing of Kv3.1b.
34070741	0	83	theme	N-Glycosylation	12:26	arg1	Processing					28:37	Compromised N-Glycosylation Processing	0:37	Compromised N-Glycosylation Processing of Kv3.1b	0:47	Compromised N-Glycosylation Processing of Kv3.1b Correlates with Perturbed Motor Neuron Structure and Locomotor Activity.
34070741	4	84	with	extension	949:957	arg1	branching					935:943	incomplete axonal branching	917:943	incomplete axonal branching	917:943	We showed that caudal primary (CaP) motor neurons of zebrafish spinal cord transiently expressing fully glycosylated (WT) Kv3.1b have stereotypical morphology, while CaP neurons expressing partially glycosylated (N220Q) Kv3.1b showed severe maldevelopment with incomplete axonal branching and extension around the ventral musculature.
34070741	6	85	theme	complex	1116:1122	arg1	N-glycans					1124:1132	complex N-glycans	1116:1132	complex N-glycans	1116:1132	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	7	86	theme	closing	1357:1363	arg1	rates					1365:1369	Opening and closing rates	1345:1369	Opening and closing rates	1345:1369	Opening and closing rates were slowed in Kv3 channels containing Kv3.1b with oligomannose, instead of complex N-glycans, which suggested a reduction in the intrinsic dynamics of the Kv3.1b α-subunit.
34070741	2	87	theme	channel	406:412	arg1	activity					414:421	channel activity	406:421	channel activity in neuroblastoma (NB) cells	406:449	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	5	88	theme	swimming	1057:1064	arg1	activity					1076:1083	impaired swimming locomotor activity	1048:1083	impaired swimming locomotor activity	1048:1083	Consequently, larvae expressing N220Q in CaP neurons had impaired swimming locomotor activity.
34070741	7	89	theme	Opening	1345:1351	arg1	rates					1365:1369	Opening and closing rates	1345:1369	Opening and closing rates	1345:1369	Opening and closing rates were slowed in Kv3 channels containing Kv3.1b with oligomannose, instead of complex N-glycans, which suggested a reduction in the intrinsic dynamics of the Kv3.1b α-subunit.
34070741	3	90	from	development	476:486	arg1	CRISPR/Cas9					561:571	CRISPR/Cas9	561:571	CRISPR/Cas9	561:571	Here we examined neuron development, localization, and activity of Kv3 channels in wildtype AB zebrafish and CRISPR/Cas9 engineered NB cells, due to perturbations in N-glycosylation processing of Kv3.1b.
34070741	3	90	from	development	476:486	arg1	zebrafish					547:555	wildtype AB zebrafish	535:555	wildtype AB zebrafish	535:555	Here we examined neuron development, localization, and activity of Kv3 channels in wildtype AB zebrafish and CRISPR/Cas9 engineered NB cells, due to perturbations in N-glycosylation processing of Kv3.1b.
34070741	2	91	theme	NB	441:442	arg1	cells					445:449	neuroblastoma (NB) cells	426:449	neuroblastoma (NB) cells	426:449	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	0	92	theme	Perturbed	65:73	arg1	Structure					88:96	Perturbed Motor Neuron Structure	65:96	Perturbed Motor Neuron Structure	65:96	Compromised N-Glycosylation Processing of Kv3.1b Correlates with Perturbed Motor Neuron Structure and Locomotor Activity.
34070741	2	93	from	activity	414:421	arg1	cells					445:449	neuroblastoma (NB) cells	426:449	neuroblastoma (NB) cells	426:449	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	6	94	from	surface	1183:1189	arg1	replacement					1101:1111	replacement	1101:1111	replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface	1101:1189	We showed that replacement of complex N-glycans with oligomannose attached to Kv3.1b and at cell surface lessened Kv3.1b dispersal to outgrowths by altering the number, size, and density of Kv3.1b-containing particles in membranes of rat neuroblastoma cells.
34070741	3	95	theme	Kv3	519:521	arg1	channels					523:530	Kv3 channels	519:530	Kv3 channels	519:530	Here we examined neuron development, localization, and activity of Kv3 channels in wildtype AB zebrafish and CRISPR/Cas9 engineered NB cells, due to perturbations in N-glycosylation processing of Kv3.1b.
34070741	5	96	theme	CaP	1032:1034	arg1	neurons					1036:1042	CaP neurons	1032:1042	CaP neurons	1032:1042	Consequently, larvae expressing N220Q in CaP neurons had impaired swimming locomotor activity.
34070741	2	97	theme	channels	358:365	arg1	sites					311:315	Vacant N-glycosylation sites	288:315	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons	288:388	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	2	97	theme	channels	358:365	arg1	channels					358:365	Kv3, voltage-gated K+ channels	336:365	channels	358:365	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	2	97	theme	channels	358:365	arg1	N229					327:330	N229	327:330	N229	327:330	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	2	97	theme	channels	358:365	arg1	N220					318:321	N220	318:321	N220	318:321	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	2	98	theme	neuroblastoma	426:438	arg1	cells					445:449	neuroblastoma (NB) cells	426:449	neuroblastoma (NB) cells	426:449	Vacant N-glycosylation sites (N220 and N229) of Kv3, voltage-gated K+ channels of high-firing neurons, deeply perturb channel activity in neuroblastoma (NB) cells.
34070741	3	99	theme	AB	544:545	arg1	zebrafish					547:555	wildtype AB zebrafish	535:555	wildtype AB zebrafish	535:555	Here we examined neuron development, localization, and activity of Kv3 channels in wildtype AB zebrafish and CRISPR/Cas9 engineered NB cells, due to perturbations in N-glycosylation processing of Kv3.1b.
34936325	4	0	theme	dual-species	769:780	arg1	biofilms					782:789	dual-species biofilms	769:789	dual-species biofilms	769:789	Our data showed that antibiotic exposure would reshape the community compositions of dual-species biofilms, and those of the extracellular polymeric substance (EPS) matrix of Pa, Psl exopolysaccharide in particular, promoted its interactions with Ab against imipenem stress.
34936325	0	1	theme	Dual-Species	86:97	arg1	Biofilms					99:106	Dual-Species Biofilms	86:106	Dual-Species Biofilms with Acinetobacter baumannii	86:135	Psl-Dependent Cooperation Contributes to Drug Resistance of Pseudomonas aeruginosa in Dual-Species Biofilms with Acinetobacter baumannii.
34936325	0	2	with	Biofilms	99:106	arg1	baumannii					127:135	Acinetobacter baumannii	113:135	Acinetobacter baumannii	113:135	Psl-Dependent Cooperation Contributes to Drug Resistance of Pseudomonas aeruginosa in Dual-Species Biofilms with Acinetobacter baumannii.
34936325	6	3	theme	potent	1188:1193	arg1	strategy					1195:1202	a potent strategy	1186:1202	a potent strategy for controlling polymicrobial biofilm related infections	1186:1259	Thus, targeting the EPS structural fibers such as Psl and extracellular DNA (eDNA) is a potent strategy for controlling polymicrobial biofilm related infections.
34936325	0	4	theme	Acinetobacter	113:125	arg1	baumannii					127:135	Acinetobacter baumannii	113:135	Acinetobacter baumannii	113:135	Psl-Dependent Cooperation Contributes to Drug Resistance of Pseudomonas aeruginosa in Dual-Species Biofilms with Acinetobacter baumannii.
34936325	4	5	theme	imipenem	942:949	arg1	stress					951:956	imipenem stress	942:956	imipenem stress	942:956	Our data showed that antibiotic exposure would reshape the community compositions of dual-species biofilms, and those of the extracellular polymeric substance (EPS) matrix of Pa, Psl exopolysaccharide in particular, promoted its interactions with Ab against imipenem stress.
34936325	6	6	theme	related	1242:1248	arg1	infections					1250:1259	polymicrobial biofilm related infections	1220:1259	polymicrobial biofilm related infections	1220:1259	Thus, targeting the EPS structural fibers such as Psl and extracellular DNA (eDNA) is a potent strategy for controlling polymicrobial biofilm related infections.
34936325	5	7	theme	Psl-dependent	1027:1039	arg1	stability					1062:1070	the Psl-dependent dual-species biofilm stability	1023:1070	the Psl-dependent dual-species biofilm stability	1023:1070	We further found other EPS structural fiber-eDNA contributed to the Psl-dependent dual-species biofilm stability under antibiotic treatment.
34936325	6	8	theme	structural	1124:1133	arg1	fibers					1135:1140	the EPS structural fibers	1116:1140	the EPS structural fibers such as Psl and extracellular DNA (eDNA)	1116:1181	Thus, targeting the EPS structural fibers such as Psl and extracellular DNA (eDNA) is a potent strategy for controlling polymicrobial biofilm related infections.
34936325	6	8	theme	structural	1124:1133	arg1	Psl					1150:1152	Psl	1150:1152	Psl	1150:1152	Thus, targeting the EPS structural fibers such as Psl and extracellular DNA (eDNA) is a potent strategy for controlling polymicrobial biofilm related infections.
34936325	6	8	theme	structural	1124:1133	arg1	DNA					1172:1174	extracellular DNA	1158:1174	extracellular DNA (eDNA)	1158:1181	Thus, targeting the EPS structural fibers such as Psl and extracellular DNA (eDNA) is a potent strategy for controlling polymicrobial biofilm related infections.
34936325	6	9	theme	polymicrobial	1220:1232	arg1	infections					1250:1259	polymicrobial biofilm related infections	1220:1259	polymicrobial biofilm related infections	1220:1259	Thus, targeting the EPS structural fibers such as Psl and extracellular DNA (eDNA) is a potent strategy for controlling polymicrobial biofilm related infections.
34936325	4	10	theme	substance	833:841	arg1	exopolysaccharide					867:883	Psl exopolysaccharide	863:883	Psl exopolysaccharide in particular	863:897	Our data showed that antibiotic exposure would reshape the community compositions of dual-species biofilms, and those of the extracellular polymeric substance (EPS) matrix of Pa, Psl exopolysaccharide in particular, promoted its interactions with Ab against imipenem stress.
34936325	4	10	theme	substance	833:841	arg1	matrix					849:854	the extracellular polymeric substance (EPS) matrix	805:854	the extracellular polymeric substance (EPS) matrix of Pa	805:860	Our data showed that antibiotic exposure would reshape the community compositions of dual-species biofilms, and those of the extracellular polymeric substance (EPS) matrix of Pa, Psl exopolysaccharide in particular, promoted its interactions with Ab against imipenem stress.
34936325	3	11	from	impacts	567:573	arg1	therapies					618:626	antibiotic therapies	607:626	antibiotic therapies	607:626	Here, we investigated the impacts of interspecies interactions on antibiotic therapies by establishing a Pa and Ab dual-species biofilm model.
34936325	3	12	theme	antibiotic	607:616	arg1	therapies					618:626	antibiotic therapies	607:626	antibiotic therapies	607:626	Here, we investigated the impacts of interspecies interactions on antibiotic therapies by establishing a Pa and Ab dual-species biofilm model.
34936325	5	13	theme	structural	986:995	arg1	fiber-eDNA					997:1006	other EPS structural fiber-eDNA	976:1006	other EPS structural fiber-eDNA contributed to the Psl-dependent dual-species biofilm stability under antibiotic treatment	976:1097	We further found other EPS structural fiber-eDNA contributed to the Psl-dependent dual-species biofilm stability under antibiotic treatment.
34936325	6	14	theme	extracellular	1158:1170	arg1	eDNA					1177:1180	eDNA	1177:1180	eDNA	1177:1180	Thus, targeting the EPS structural fibers such as Psl and extracellular DNA (eDNA) is a potent strategy for controlling polymicrobial biofilm related infections.
34936325	6	14	theme	extracellular	1158:1170	arg1	DNA					1172:1174	extracellular DNA	1158:1174	extracellular DNA (eDNA)	1158:1181	Thus, targeting the EPS structural fibers such as Psl and extracellular DNA (eDNA) is a potent strategy for controlling polymicrobial biofilm related infections.
34936325	3	15	theme	interspecies	578:589	arg1	interactions					591:602	interspecies interactions	578:602	interspecies interactions	578:602	Here, we investigated the impacts of interspecies interactions on antibiotic therapies by establishing a Pa and Ab dual-species biofilm model.
34936325	4	16	theme	Psl	863:865	arg1	exopolysaccharide					867:883	Psl exopolysaccharide	863:883	Psl exopolysaccharide in particular	863:897	Our data showed that antibiotic exposure would reshape the community compositions of dual-species biofilms, and those of the extracellular polymeric substance (EPS) matrix of Pa, Psl exopolysaccharide in particular, promoted its interactions with Ab against imipenem stress.
34936325	4	16	theme	Psl	863:865	arg1	matrix					849:854	the extracellular polymeric substance (EPS) matrix	805:854	the extracellular polymeric substance (EPS) matrix of Pa	805:860	Our data showed that antibiotic exposure would reshape the community compositions of dual-species biofilms, and those of the extracellular polymeric substance (EPS) matrix of Pa, Psl exopolysaccharide in particular, promoted its interactions with Ab against imipenem stress.
34936325	0	17	from	Resistance	46:55	arg1	Biofilms					99:106	Dual-Species Biofilms	86:106	Dual-Species Biofilms with Acinetobacter baumannii	86:135	Psl-Dependent Cooperation Contributes to Drug Resistance of Pseudomonas aeruginosa in Dual-Species Biofilms with Acinetobacter baumannii.
34936325	3	18	theme	interactions	591:602	arg1	impacts					567:573	the impacts	563:573	the impacts of interspecies interactions on antibiotic therapies	563:626	Here, we investigated the impacts of interspecies interactions on antibiotic therapies by establishing a Pa and Ab dual-species biofilm model.
34936325	1	19	theme	baumannii	200:208	arg1	Co-infection					138:149	Co-infection	138:149	Co-infection of Pseudomonas aeruginosa (Pa) and Acinetobacter baumannii (Ab)	138:213	Co-infection of Pseudomonas aeruginosa (Pa) and Acinetobacter baumannii (Ab) is frequently observed in intensive care unit (ICU) patients but difficult to eliminate.
34936325	6	20	theme	biofilm	1234:1240	arg1	infections					1250:1259	polymicrobial biofilm related infections	1220:1259	polymicrobial biofilm related infections	1220:1259	Thus, targeting the EPS structural fibers such as Psl and extracellular DNA (eDNA) is a potent strategy for controlling polymicrobial biofilm related infections.
34936325	0	21	theme	Psl-Dependent	0:12	arg1	Cooperation					14:24	Psl-Dependent Cooperation	0:24	Psl-Dependent Cooperation	0:24	Psl-Dependent Cooperation Contributes to Drug Resistance of Pseudomonas aeruginosa in Dual-Species Biofilms with Acinetobacter baumannii.
34936325	2	22	theme	clinical	312:319	arg1	practice					321:328	Current clinical practice	304:328	Current clinical practice based on microbial population characterization and single-species-based antibiotic resistance profiling	304:432	Current clinical practice based on microbial population characterization and single-species-based antibiotic resistance profiling has ignored the potential interspecies interactions, which might lead to novel drug-resistance phenotypes.
34936325	2	23	theme	Current	304:310	arg1	practice					321:328	Current clinical practice	304:328	Current clinical practice based on microbial population characterization and single-species-based antibiotic resistance profiling	304:432	Current clinical practice based on microbial population characterization and single-species-based antibiotic resistance profiling has ignored the potential interspecies interactions, which might lead to novel drug-resistance phenotypes.
34936325	5	24	theme	other	976:980	arg1	fiber-eDNA					997:1006	other EPS structural fiber-eDNA	976:1006	other EPS structural fiber-eDNA contributed to the Psl-dependent dual-species biofilm stability under antibiotic treatment	976:1097	We further found other EPS structural fiber-eDNA contributed to the Psl-dependent dual-species biofilm stability under antibiotic treatment.
34936325	5	25	theme	biofilm	1054:1060	arg1	stability					1062:1070	the Psl-dependent dual-species biofilm stability	1023:1070	the Psl-dependent dual-species biofilm stability	1023:1070	We further found other EPS structural fiber-eDNA contributed to the Psl-dependent dual-species biofilm stability under antibiotic treatment.
34936325	4	26	theme	extracellular	809:821	arg1	exopolysaccharide					867:883	Psl exopolysaccharide	863:883	Psl exopolysaccharide in particular	863:897	Our data showed that antibiotic exposure would reshape the community compositions of dual-species biofilms, and those of the extracellular polymeric substance (EPS) matrix of Pa, Psl exopolysaccharide in particular, promoted its interactions with Ab against imipenem stress.
34936325	4	26	theme	extracellular	809:821	arg1	matrix					849:854	the extracellular polymeric substance (EPS) matrix	805:854	the extracellular polymeric substance (EPS) matrix of Pa	805:860	Our data showed that antibiotic exposure would reshape the community compositions of dual-species biofilms, and those of the extracellular polymeric substance (EPS) matrix of Pa, Psl exopolysaccharide in particular, promoted its interactions with Ab against imipenem stress.
34936325	2	27	theme	interspecies	460:471	arg1	interactions					473:484	the potential interspecies interactions	446:484	the potential interspecies interactions	446:484	Current clinical practice based on microbial population characterization and single-species-based antibiotic resistance profiling has ignored the potential interspecies interactions, which might lead to novel drug-resistance phenotypes.
34936325	4	28	theme	biofilms	782:789	arg1	compositions					753:764	the community compositions	739:764	the community compositions of dual-species biofilms	739:789	Our data showed that antibiotic exposure would reshape the community compositions of dual-species biofilms, and those of the extracellular polymeric substance (EPS) matrix of Pa, Psl exopolysaccharide in particular, promoted its interactions with Ab against imipenem stress.
34936325	4	29	theme	antibiotic	705:714	arg1	exposure					716:723	antibiotic exposure	705:723	antibiotic exposure	705:723	Our data showed that antibiotic exposure would reshape the community compositions of dual-species biofilms, and those of the extracellular polymeric substance (EPS) matrix of Pa, Psl exopolysaccharide in particular, promoted its interactions with Ab against imipenem stress.
34936325	3	30	theme	Ab	653:654	arg1	model					677:681	a Pa and Ab dual-species biofilm model	644:681	a Pa and Ab dual-species biofilm model	644:681	Here, we investigated the impacts of interspecies interactions on antibiotic therapies by establishing a Pa and Ab dual-species biofilm model.
34936325	2	31	theme	potential	450:458	arg1	interactions					473:484	the potential interspecies interactions	446:484	the potential interspecies interactions	446:484	Current clinical practice based on microbial population characterization and single-species-based antibiotic resistance profiling has ignored the potential interspecies interactions, which might lead to novel drug-resistance phenotypes.
34936325	2	32	theme	drug-resistance	513:527	arg1	phenotypes					529:538	novel drug-resistance phenotypes	507:538	novel drug-resistance phenotypes	507:538	Current clinical practice based on microbial population characterization and single-species-based antibiotic resistance profiling has ignored the potential interspecies interactions, which might lead to novel drug-resistance phenotypes.
34936325	0	33	theme	Drug	41:44	arg1	Resistance					46:55	Drug Resistance	41:55	Drug Resistance of Pseudomonas aeruginosa in Dual-Species Biofilms with Acinetobacter baumannii	41:135	Psl-Dependent Cooperation Contributes to Drug Resistance of Pseudomonas aeruginosa in Dual-Species Biofilms with Acinetobacter baumannii.
34936325	3	34	theme	dual-species	656:667	arg1	model					677:681	a Pa and Ab dual-species biofilm model	644:681	a Pa and Ab dual-species biofilm model	644:681	Here, we investigated the impacts of interspecies interactions on antibiotic therapies by establishing a Pa and Ab dual-species biofilm model.
34936325	4	35	theme	polymeric	823:831	arg1	exopolysaccharide					867:883	Psl exopolysaccharide	863:883	Psl exopolysaccharide in particular	863:897	Our data showed that antibiotic exposure would reshape the community compositions of dual-species biofilms, and those of the extracellular polymeric substance (EPS) matrix of Pa, Psl exopolysaccharide in particular, promoted its interactions with Ab against imipenem stress.
34936325	4	35	theme	polymeric	823:831	arg1	matrix					849:854	the extracellular polymeric substance (EPS) matrix	805:854	the extracellular polymeric substance (EPS) matrix of Pa	805:860	Our data showed that antibiotic exposure would reshape the community compositions of dual-species biofilms, and those of the extracellular polymeric substance (EPS) matrix of Pa, Psl exopolysaccharide in particular, promoted its interactions with Ab against imipenem stress.
34936325	2	36	theme	novel	507:511	arg1	phenotypes					529:538	novel drug-resistance phenotypes	507:538	novel drug-resistance phenotypes	507:538	Current clinical practice based on microbial population characterization and single-species-based antibiotic resistance profiling has ignored the potential interspecies interactions, which might lead to novel drug-resistance phenotypes.
34936325	3	37	theme	Pa	646:647	arg1	model					677:681	a Pa and Ab dual-species biofilm model	644:681	a Pa and Ab dual-species biofilm model	644:681	Here, we investigated the impacts of interspecies interactions on antibiotic therapies by establishing a Pa and Ab dual-species biofilm model.
34936325	4	38	from	exopolysaccharide	867:883	arg1	particular					888:897	particular	888:897	particular	888:897	Our data showed that antibiotic exposure would reshape the community compositions of dual-species biofilms, and those of the extracellular polymeric substance (EPS) matrix of Pa, Psl exopolysaccharide in particular, promoted its interactions with Ab against imipenem stress.
34936325	2	39	theme	microbial	339:347	arg1	population					349:358	microbial population	339:358	microbial population	339:358	Current clinical practice based on microbial population characterization and single-species-based antibiotic resistance profiling has ignored the potential interspecies interactions, which might lead to novel drug-resistance phenotypes.
34936325	4	40	theme	community	743:751	arg1	compositions					753:764	the community compositions	739:764	the community compositions of dual-species biofilms	739:789	Our data showed that antibiotic exposure would reshape the community compositions of dual-species biofilms, and those of the extracellular polymeric substance (EPS) matrix of Pa, Psl exopolysaccharide in particular, promoted its interactions with Ab against imipenem stress.
34936325	5	41	theme	EPS	982:984	arg1	fiber-eDNA					997:1006	other EPS structural fiber-eDNA	976:1006	other EPS structural fiber-eDNA contributed to the Psl-dependent dual-species biofilm stability under antibiotic treatment	976:1097	We further found other EPS structural fiber-eDNA contributed to the Psl-dependent dual-species biofilm stability under antibiotic treatment.
34936325	2	42	dep	population	349:358	arg1	characterization					360:375	characterization	360:375	characterization	360:375	Current clinical practice based on microbial population characterization and single-species-based antibiotic resistance profiling has ignored the potential interspecies interactions, which might lead to novel drug-resistance phenotypes.
34936325	4	43	theme	EPS	844:846	arg1	exopolysaccharide					867:883	Psl exopolysaccharide	863:883	Psl exopolysaccharide in particular	863:897	Our data showed that antibiotic exposure would reshape the community compositions of dual-species biofilms, and those of the extracellular polymeric substance (EPS) matrix of Pa, Psl exopolysaccharide in particular, promoted its interactions with Ab against imipenem stress.
34936325	4	43	theme	EPS	844:846	arg1	matrix					849:854	the extracellular polymeric substance (EPS) matrix	805:854	the extracellular polymeric substance (EPS) matrix of Pa	805:860	Our data showed that antibiotic exposure would reshape the community compositions of dual-species biofilms, and those of the extracellular polymeric substance (EPS) matrix of Pa, Psl exopolysaccharide in particular, promoted its interactions with Ab against imipenem stress.
34936325	1	44	located	observed	229:236	arg2	Co-infection					138:149	Co-infection	138:149	Co-infection of Pseudomonas aeruginosa (Pa) and Acinetobacter baumannii (Ab)	138:213	Co-infection of Pseudomonas aeruginosa (Pa) and Acinetobacter baumannii (Ab) is frequently observed in intensive care unit (ICU) patients but difficult to eliminate.
34936325	1	44	located	observed	229:236	arg1	patients					267:274	intensive care unit (ICU) patients	241:274	intensive care unit (ICU) patients	241:274	Co-infection of Pseudomonas aeruginosa (Pa) and Acinetobacter baumannii (Ab) is frequently observed in intensive care unit (ICU) patients but difficult to eliminate.
34936325	5	45	theme	antibiotic	1078:1087	arg1	treatment					1089:1097	antibiotic treatment	1078:1097	antibiotic treatment	1078:1097	We further found other EPS structural fiber-eDNA contributed to the Psl-dependent dual-species biofilm stability under antibiotic treatment.
34936325	1	46	theme	aeruginosa	166:175	arg1	Co-infection					138:149	Co-infection	138:149	Co-infection of Pseudomonas aeruginosa (Pa) and Acinetobacter baumannii (Ab)	138:213	Co-infection of Pseudomonas aeruginosa (Pa) and Acinetobacter baumannii (Ab) is frequently observed in intensive care unit (ICU) patients but difficult to eliminate.
34936325	1	47	theme	intensive	241:249	arg1	ICU					262:264	ICU	262:264	ICU	262:264	Co-infection of Pseudomonas aeruginosa (Pa) and Acinetobacter baumannii (Ab) is frequently observed in intensive care unit (ICU) patients but difficult to eliminate.
34936325	1	47	theme	intensive	241:249	arg1	unit					256:259	intensive care unit	241:259	intensive care unit (ICU) patients	241:274	Co-infection of Pseudomonas aeruginosa (Pa) and Acinetobacter baumannii (Ab) is frequently observed in intensive care unit (ICU) patients but difficult to eliminate.
34936325	6	48	theme	EPS	1120:1122	arg1	fibers					1135:1140	the EPS structural fibers	1116:1140	the EPS structural fibers such as Psl and extracellular DNA (eDNA)	1116:1181	Thus, targeting the EPS structural fibers such as Psl and extracellular DNA (eDNA) is a potent strategy for controlling polymicrobial biofilm related infections.
34936325	6	48	theme	EPS	1120:1122	arg1	Psl					1150:1152	Psl	1150:1152	Psl	1150:1152	Thus, targeting the EPS structural fibers such as Psl and extracellular DNA (eDNA) is a potent strategy for controlling polymicrobial biofilm related infections.
34936325	6	48	theme	EPS	1120:1122	arg1	DNA					1172:1174	extracellular DNA	1158:1174	extracellular DNA (eDNA)	1158:1181	Thus, targeting the EPS structural fibers such as Psl and extracellular DNA (eDNA) is a potent strategy for controlling polymicrobial biofilm related infections.
34936325	3	49	theme	biofilm	669:675	arg1	model					677:681	a Pa and Ab dual-species biofilm model	644:681	a Pa and Ab dual-species biofilm model	644:681	Here, we investigated the impacts of interspecies interactions on antibiotic therapies by establishing a Pa and Ab dual-species biofilm model.
34936325	2	50	theme	antibiotic	402:411	arg1	resistance					413:422	single-species-based antibiotic resistance	381:422	single-species-based antibiotic resistance	381:422	Current clinical practice based on microbial population characterization and single-species-based antibiotic resistance profiling has ignored the potential interspecies interactions, which might lead to novel drug-resistance phenotypes.
34936325	1	51	theme	care	251:254	arg1	ICU					262:264	ICU	262:264	ICU	262:264	Co-infection of Pseudomonas aeruginosa (Pa) and Acinetobacter baumannii (Ab) is frequently observed in intensive care unit (ICU) patients but difficult to eliminate.
34936325	1	51	theme	care	251:254	arg1	unit					256:259	intensive care unit	241:259	intensive care unit (ICU) patients	241:274	Co-infection of Pseudomonas aeruginosa (Pa) and Acinetobacter baumannii (Ab) is frequently observed in intensive care unit (ICU) patients but difficult to eliminate.
34936325	0	52	theme	aeruginosa	72:81	arg1	Resistance					46:55	Drug Resistance	41:55	Drug Resistance of Pseudomonas aeruginosa in Dual-Species Biofilms with Acinetobacter baumannii	41:135	Psl-Dependent Cooperation Contributes to Drug Resistance of Pseudomonas aeruginosa in Dual-Species Biofilms with Acinetobacter baumannii.
34936325	5	53	theme	dual-species	1041:1052	arg1	stability					1062:1070	the Psl-dependent dual-species biofilm stability	1023:1070	the Psl-dependent dual-species biofilm stability	1023:1070	We further found other EPS structural fiber-eDNA contributed to the Psl-dependent dual-species biofilm stability under antibiotic treatment.
34936325	4	54	theme	Pa	859:860	arg1	exopolysaccharide					867:883	Psl exopolysaccharide	863:883	Psl exopolysaccharide in particular	863:897	Our data showed that antibiotic exposure would reshape the community compositions of dual-species biofilms, and those of the extracellular polymeric substance (EPS) matrix of Pa, Psl exopolysaccharide in particular, promoted its interactions with Ab against imipenem stress.
34936325	4	54	theme	Pa	859:860	arg1	matrix					849:854	the extracellular polymeric substance (EPS) matrix	805:854	the extracellular polymeric substance (EPS) matrix of Pa	805:860	Our data showed that antibiotic exposure would reshape the community compositions of dual-species biofilms, and those of the extracellular polymeric substance (EPS) matrix of Pa, Psl exopolysaccharide in particular, promoted its interactions with Ab against imipenem stress.
34936325	2	55	theme	single-species-based	381:400	arg1	resistance					413:422	single-species-based antibiotic resistance	381:422	single-species-based antibiotic resistance	381:422	Current clinical practice based on microbial population characterization and single-species-based antibiotic resistance profiling has ignored the potential interspecies interactions, which might lead to novel drug-resistance phenotypes.
34936325	4	56	with	interactions	913:924	arg1	Ab					931:932	Ab	931:932	Ab	931:932	Our data showed that antibiotic exposure would reshape the community compositions of dual-species biofilms, and those of the extracellular polymeric substance (EPS) matrix of Pa, Psl exopolysaccharide in particular, promoted its interactions with Ab against imipenem stress.
34936325	1	57	theme	unit	256:259	arg1	patients					267:274	intensive care unit (ICU) patients	241:274	intensive care unit (ICU) patients	241:274	Co-infection of Pseudomonas aeruginosa (Pa) and Acinetobacter baumannii (Ab) is frequently observed in intensive care unit (ICU) patients but difficult to eliminate.
34931502	10	0	theme	composite	1860:1868	arg1	nanogels					1870:1877	tilmicosin composite nanogels	1849:1877	tilmicosin composite nanogels against the SASCVs strain	1849:1903	The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain were 2, 1, and 1 µg/mL, respectively.
34931502	3	1	theme	COS	584:586	arg1	nanogels					636:643	Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels	540:643	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels	532:643	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers).
34931502	1	2	theme	SASCVs	312:317	arg1	mastitis					320:327	Staphylococcus aureus small colony variants (SASCVs) mastitis	267:327	Staphylococcus aureus small colony variants (SASCVs) mastitis	267:327	BACKGROUND The poor bioadhesion capacity of tilmicosin resulting in treatment failure for Staphylococcus aureus small colony variants (SASCVs) mastitis.
34931502	7	3	theme	polymeric	1435:1443	arg1	networks					1445:1452	nano-sized crosslinked polymeric networks	1412:1452	nano-sized crosslinked polymeric networks	1412:1452	Scanning electron microscopy showed that tilmicosin might be incorporated in nano-sized crosslinked polymeric networks.
34931502	9	4	theme	tilmicosin	1633:1642	arg1	2.13					1705:1708	2.13	1705:1708	2.13	1705:1708	The inhibition zone of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels were 2.13 ± 0.07, 3.35 ± 0.11, and 1.46 ± 0.04 cm, respectively.
34931502	9	4	theme	tilmicosin	1633:1642	arg1	zone					1618:1621	The inhibition zone	1603:1621	The inhibition zone of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels	1603:1698	The inhibition zone of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels were 2.13 ± 0.07, 3.35 ± 0.11, and 1.46 ± 0.04 cm, respectively.
34931502	3	5	theme	CMC	621:623	arg1	nanogels					636:643	Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels	540:643	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels	532:643	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers).
34931502	7	6	theme	nano-sized	1412:1421	arg1	networks					1445:1452	nano-sized crosslinked polymeric networks	1412:1452	nano-sized crosslinked polymeric networks	1412:1452	Scanning electron microscopy showed that tilmicosin might be incorporated in nano-sized crosslinked polymeric networks.
34931502	9	7	theme	tilmicosin	1645:1654	arg1	2.13					1705:1708	2.13	1705:1708	2.13	1705:1708	The inhibition zone of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels were 2.13 ± 0.07, 3.35 ± 0.11, and 1.46 ± 0.04 cm, respectively.
34931502	9	7	theme	tilmicosin	1645:1654	arg1	zone					1618:1621	The inhibition zone	1603:1621	The inhibition zone of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels	1603:1698	The inhibition zone of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels were 2.13 ± 0.07, 3.35 ± 0.11, and 1.46 ± 0.04 cm, respectively.
34931502	6	8	theme	composite	1190:1198	arg1	nanogels					1200:1207	the optimized tilmicosin composite nanogels	1165:1207	the optimized tilmicosin composite nanogels	1165:1207	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	10	9	theme	SASCVs	1891:1896	arg1	strain					1898:1903	the SASCVs strain	1887:1903	the SASCVs strain	1887:1903	The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain were 2, 1, and 1 µg/mL, respectively.
34931502	3	10	theme	cellulose	610:618	arg1	nanogels					636:643	Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels	540:643	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels	532:643	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers).
34931502	1	11	theme	tilmicosin	221:230	arg1	capacity					209:216	The poor bioadhesion capacity	188:216	The poor bioadhesion capacity of tilmicosin resulting in treatment failure for Staphylococcus aureus small colony variants (SASCVs) mastitis	188:327	BACKGROUND The poor bioadhesion capacity of tilmicosin resulting in treatment failure for Staphylococcus aureus small colony variants (SASCVs) mastitis.
34931502	4	12	theme	nanogels	924:931	arg1	bioadhesion					860:870	bioadhesion	860:870	bioadhesion	860:870	The formation mechanism, structural characteristics, bioadhesion, and antibacterial activity of tilmicosin composite nanogels were studied systematically.
34931502	4	12	theme	nanogels	924:931	arg1	mechanism					821:829	The formation mechanism	807:829	The formation mechanism	807:829	The formation mechanism, structural characteristics, bioadhesion, and antibacterial activity of tilmicosin composite nanogels were studied systematically.
34931502	4	12	theme	nanogels	924:931	arg1	characteristics					843:857	structural characteristics	832:857	structural characteristics	832:857	The formation mechanism, structural characteristics, bioadhesion, and antibacterial activity of tilmicosin composite nanogels were studied systematically.
34931502	4	12	theme	nanogels	924:931	arg1	activity					891:898	antibacterial activity	877:898	antibacterial activity	877:898	The formation mechanism, structural characteristics, bioadhesion, and antibacterial activity of tilmicosin composite nanogels were studied systematically.
34931502	7	13	theme	electron	1344:1351	arg1	microscopy					1353:1362	Scanning electron microscopy	1335:1362	Scanning electron microscopy	1335:1362	Scanning electron microscopy showed that tilmicosin might be incorporated in nano-sized crosslinked polymeric networks.
34931502	11	14	theme	antibacterial	2036:2048	arg1	activity					2050:2057	the antibacterial activity	2032:2057	the antibacterial activity against the SASCVs strain	2032:2083	The in vitro time-killing curves showed that the tilmicosin composite nanogels increased the antibacterial activity against the SASCVs strain.
34931502	11	15	theme	SASCVs	2071:2076	arg1	strain					2078:2083	the SASCVs strain	2067:2083	the SASCVs strain	2067:2083	The in vitro time-killing curves showed that the tilmicosin composite nanogels increased the antibacterial activity against the SASCVs strain.
34931502	9	16	theme	tilmicosin	1670:1679	arg1	nanogels					1691:1698	tilmicosin composite nanogels	1670:1698	tilmicosin composite nanogels	1670:1698	The inhibition zone of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels were 2.13 ± 0.07, 3.35 ± 0.11, and 1.46 ± 0.04 cm, respectively.
34931502	3	17	theme	ionic	786:790	arg1	tripolyphosphate					762:777	sodium tripolyphosphate	755:777	sodium tripolyphosphate (TPP) (ionic crosslinkers)	755:804	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers).
34931502	3	17	theme	ionic	786:790	arg1	crosslinkers					792:803	ionic crosslinkers	786:803	ionic crosslinkers	786:803	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers).
34931502	0	18	theme	composite	157:165	arg1	nanogels					167:174	chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels	101:174	chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels	101:174	Enhanced antibacterial activity of tilmicosin against Staphylococcus aureus small colony variants by chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels.
34931502	3	19	theme	composite	626:634	arg1	nanogels					636:643	Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels	540:643	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels	532:643	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers).
34931502	9	20	theme	nanogels	1691:1698	arg1	2.13					1705:1708	2.13	1705:1708	2.13	1705:1708	The inhibition zone of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels were 2.13 ± 0.07, 3.35 ± 0.11, and 1.46 ± 0.04 cm, respectively.
34931502	9	20	theme	nanogels	1691:1698	arg1	zone					1618:1621	The inhibition zone	1603:1621	The inhibition zone of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels	1603:1698	The inhibition zone of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels were 2.13 ± 0.07, 3.35 ± 0.11, and 1.46 ± 0.04 cm, respectively.
34931502	3	21	theme	electrostatic	667:679	arg1	interaction					681:691	an electrostatic interaction	664:691	an electrostatic interaction between COS (positive charge) and CMC (negative charge)	664:747	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers).
34931502	1	22	theme	Staphylococcus	267:280	arg1	mastitis					320:327	Staphylococcus aureus small colony variants (SASCVs) mastitis	267:327	Staphylococcus aureus small colony variants (SASCVs) mastitis	267:327	BACKGROUND The poor bioadhesion capacity of tilmicosin resulting in treatment failure for Staphylococcus aureus small colony variants (SASCVs) mastitis.
34931502	10	23	theme	inhibitory	1777:1786	arg1	µg/mL					1922:1926	2, 1, and 1 µg/mL	1910:1926	2, 1, and 1 µg/mL	1910:1926	The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain were 2, 1, and 1 µg/mL, respectively.
34931502	10	23	theme	inhibitory	1777:1786	arg1	concentration					1788:1800	The minimum inhibitory concentration	1765:1800	The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain	1765:1903	The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain were 2, 1, and 1 µg/mL, respectively.
34931502	11	24	dep	in	1947:1948	arg1	vitro					1950:1954	vitro	1950:1954	vitro	1950:1954	The in vitro time-killing curves showed that the tilmicosin composite nanogels increased the antibacterial activity against the SASCVs strain.
34931502	6	25	theme	±	1220:1220	arg1	nm					1226:1227	357.4 ± 2.6 nm	1214:1227	357.4 ± 2.6 nm	1214:1227	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	6	25	theme	±	1220:1220	arg1	size					1071:1074	The size	1067:1074	The size	1067:1074	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	2	26	dep	OBJECTIVES	330:339	arg1	aimed					352:356	aimed	352:356	aimed to increase the bioadhesion capacity of tilmicosin for the SASCVs strain and improve the antibacterial effect of tilmicosin against cow mastitis caused by the SASCVs strain	352:529	OBJECTIVES This study aimed to increase the bioadhesion capacity of tilmicosin for the SASCVs strain and improve the antibacterial effect of tilmicosin against cow mastitis caused by the SASCVs strain.
34931502	0	27	theme	small	76:80	arg1	variants					89:96	Staphylococcus aureus small colony variants	54:96	Staphylococcus aureus small colony variants	54:96	Enhanced antibacterial activity of tilmicosin against Staphylococcus aureus small colony variants by chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels.
34931502	1	28	theme	poor	192:195	arg1	capacity					209:216	The poor bioadhesion capacity	188:216	The poor bioadhesion capacity of tilmicosin resulting in treatment failure for Staphylococcus aureus small colony variants (SASCVs) mastitis	188:327	BACKGROUND The poor bioadhesion capacity of tilmicosin resulting in treatment failure for Staphylococcus aureus small colony variants (SASCVs) mastitis.
34931502	6	29	theme	sedimentation	1307:1319	arg1	rate					1321:1324	the sedimentation rate	1303:1324	the sedimentation rate	1303:1324	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	6	29	theme	sedimentation	1307:1319	arg1	one					1330:1332	one	1330:1332	one	1330:1332	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	8	30	theme	adhesive	1465:1472	arg1	studies					1474:1480	adhesive studies	1465:1480	adhesive studies	1465:1480	Moreover, adhesive studies suggested that tilmicosin composite nanogels could enhance the bioadhesion capacity of tilmicosin for the SASCVs strain.
34931502	4	31	theme	formation	811:819	arg1	mechanism					821:829	The formation mechanism	807:829	The formation mechanism	807:829	The formation mechanism, structural characteristics, bioadhesion, and antibacterial activity of tilmicosin composite nanogels were studied systematically.
34931502	0	32	theme	chitosan	101:108	arg1	nanogels					167:174	chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels	101:174	chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels	101:174	Enhanced antibacterial activity of tilmicosin against Staphylococcus aureus small colony variants by chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels.
34931502	6	33	theme	nanogels	1200:1207	arg1	nm					1226:1227	357.4 ± 2.6 nm	1214:1227	357.4 ± 2.6 nm	1214:1227	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	6	33	theme	nanogels	1200:1207	arg1	size					1071:1074	The size	1067:1074	The size	1067:1074	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	6	33	theme	nanogels	1200:1207	arg1	capacity					1111:1118	loading capacity	1103:1118	loading capacity	1103:1118	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	6	33	theme	nanogels	1200:1207	arg1	potential					1152:1160	zeta potential	1147:1160	zeta potential	1147:1160	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	6	33	theme	nanogels	1200:1207	arg1	efficiency					1091:1100	encapsulation efficiency	1077:1100	encapsulation efficiency	1077:1100	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	6	33	theme	nanogels	1200:1207	arg1	index					1136:1140	polydispersity index	1121:1140	polydispersity index	1121:1140	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	2	34	theme	antibacterial	447:459	arg1	effect					461:466	the antibacterial effect	443:466	the antibacterial effect of tilmicosin against cow mastitis caused by the SASCVs strain	443:529	OBJECTIVES This study aimed to increase the bioadhesion capacity of tilmicosin for the SASCVs strain and improve the antibacterial effect of tilmicosin against cow mastitis caused by the SASCVs strain.
34931502	0	35	theme	carboxymethyl	133:145	arg1	nanogels					167:174	chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels	101:174	chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels	101:174	Enhanced antibacterial activity of tilmicosin against Staphylococcus aureus small colony variants by chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels.
34931502	8	36	theme	tilmicosin	1497:1506	arg1	nanogels					1518:1525	tilmicosin composite nanogels	1497:1525	tilmicosin composite nanogels	1497:1525	Moreover, adhesive studies suggested that tilmicosin composite nanogels could enhance the bioadhesion capacity of tilmicosin for the SASCVs strain.
34931502	11	37	theme	in	1947:1948	arg1	curves					1969:1974	The in vitro time-killing curves	1943:1974	The in vitro time-killing curves	1943:1974	The in vitro time-killing curves showed that the tilmicosin composite nanogels increased the antibacterial activity against the SASCVs strain.
34931502	4	38	theme	tilmicosin	903:912	arg1	nanogels					924:931	tilmicosin composite nanogels	903:931	tilmicosin composite nanogels	903:931	The formation mechanism, structural characteristics, bioadhesion, and antibacterial activity of tilmicosin composite nanogels were studied systematically.
34931502	5	39	dep	RESULTS	962:968	arg1	comprised					1000:1008	comprised	1000:1008	was comprised of 50 mg/mL (COS), 32 mg/mL (CMC), and 0.25 mg/mL (TPP)	996:1064	RESULTS The optimized formulation was comprised of 50 mg/mL (COS), 32 mg/mL (CMC), and 0.25 mg/mL (TPP).
34931502	10	40	theme	tilmicosin	1812:1821	arg1	µg/mL					1922:1926	2, 1, and 1 µg/mL	1910:1926	2, 1, and 1 µg/mL	1910:1926	The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain were 2, 1, and 1 µg/mL, respectively.
34931502	10	40	theme	tilmicosin	1812:1821	arg1	concentration					1788:1800	The minimum inhibitory concentration	1765:1800	The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain	1765:1903	The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain were 2, 1, and 1 µg/mL, respectively.
34931502	0	41	theme	tilmicosin	35:44	arg1	activity					23:30	antibacterial activity	9:30	antibacterial activity of tilmicosin against Staphylococcus aureus small colony variants	9:96	Enhanced antibacterial activity of tilmicosin against Staphylococcus aureus small colony variants by chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels.
34931502	2	42	theme	SASCVs	517:522	arg1	strain					524:529	the SASCVs strain	513:529	the SASCVs strain	513:529	OBJECTIVES This study aimed to increase the bioadhesion capacity of tilmicosin for the SASCVs strain and improve the antibacterial effect of tilmicosin against cow mastitis caused by the SASCVs strain.
34931502	8	43	theme	bioadhesion	1545:1555	arg1	capacity					1557:1564	the bioadhesion capacity	1541:1564	the bioadhesion capacity of tilmicosin for the SASCVs strain	1541:1600	Moreover, adhesive studies suggested that tilmicosin composite nanogels could enhance the bioadhesion capacity of tilmicosin for the SASCVs strain.
34931502	12	44	theme	tilmicosin	2154:2163	arg1	nanogels					2175:2182	tilmicosin composite nanogels	2154:2182	tilmicosin composite nanogels	2154:2182	CONCLUSIONS This study provides a potential strategy for developing tilmicosin composite nanogels to treat cow mastitis caused by the SASCVs strain.
34931502	3	45	theme	chitosan	558:565	arg1	nanogels					636:643	Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels	540:643	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels	532:643	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers).
34931502	0	46	theme	Staphylococcus	54:67	arg1	variants					89:96	Staphylococcus aureus small colony variants	54:96	Staphylococcus aureus small colony variants	54:96	Enhanced antibacterial activity of tilmicosin against Staphylococcus aureus small colony variants by chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels.
34931502	10	47	theme	native	1805:1810	arg1	tilmicosin					1812:1821	native tilmicosin	1805:1821	native tilmicosin	1805:1821	The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain were 2, 1, and 1 µg/mL, respectively.
34931502	12	48	dep	CONCLUSIONS	2086:2096	arg1	provides					2109:2116	provides	2109:2116	provides a potential strategy for developing tilmicosin composite nanogels to treat cow mastitis caused by the SASCVs strain	2109:2232	CONCLUSIONS This study provides a potential strategy for developing tilmicosin composite nanogels to treat cow mastitis caused by the SASCVs strain.
34931502	10	49	theme	tilmicosin	1824:1833	arg1	µg/mL					1922:1926	2, 1, and 1 µg/mL	1910:1926	2, 1, and 1 µg/mL	1910:1926	The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain were 2, 1, and 1 µg/mL, respectively.
34931502	10	49	theme	tilmicosin	1824:1833	arg1	concentration					1788:1800	The minimum inhibitory concentration	1765:1800	The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain	1765:1903	The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain were 2, 1, and 1 µg/mL, respectively.
34931502	12	50	theme	potential	2120:2128	arg1	strategy					2130:2137	a potential strategy	2118:2137	a potential strategy for developing tilmicosin composite nanogels to treat cow mastitis caused by the SASCVs strain	2118:2232	CONCLUSIONS This study provides a potential strategy for developing tilmicosin composite nanogels to treat cow mastitis caused by the SASCVs strain.
34931502	1	51	theme	colony	295:300	arg1	mastitis					320:327	Staphylococcus aureus small colony variants (SASCVs) mastitis	267:327	Staphylococcus aureus small colony variants (SASCVs) mastitis	267:327	BACKGROUND The poor bioadhesion capacity of tilmicosin resulting in treatment failure for Staphylococcus aureus small colony variants (SASCVs) mastitis.
34931502	8	52	theme	SASCVs	1588:1593	arg1	strain					1595:1600	the SASCVs strain	1584:1600	the SASCVs strain	1584:1600	Moreover, adhesive studies suggested that tilmicosin composite nanogels could enhance the bioadhesion capacity of tilmicosin for the SASCVs strain.
34931502	10	53	theme	nanogels	1870:1877	arg1	µg/mL					1922:1926	2, 1, and 1 µg/mL	1910:1926	2, 1, and 1 µg/mL	1910:1926	The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain were 2, 1, and 1 µg/mL, respectively.
34931502	10	53	theme	nanogels	1870:1877	arg1	concentration					1788:1800	The minimum inhibitory concentration	1765:1800	The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain	1765:1903	The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain were 2, 1, and 1 µg/mL, respectively.
34931502	6	54	theme	0.11	1256:1259	arg1	±					1261:1261	0.11 ± 0.01	1256:1266	0.11 ± 0.01	1256:1266	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	7	55	theme	crosslinked	1423:1433	arg1	networks					1445:1452	nano-sized crosslinked polymeric networks	1412:1452	nano-sized crosslinked polymeric networks	1412:1452	Scanning electron microscopy showed that tilmicosin might be incorporated in nano-sized crosslinked polymeric networks.
34931502	9	56	theme	native	1626:1631	arg1	tilmicosin					1633:1642	native tilmicosin	1626:1642	native tilmicosin	1626:1642	The inhibition zone of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels were 2.13 ± 0.07, 3.35 ± 0.11, and 1.46 ± 0.04 cm, respectively.
34931502	6	57	theme	loading	1103:1109	arg1	capacity					1111:1118	loading capacity	1103:1118	loading capacity	1103:1118	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	10	58	theme	tilmicosin	1849:1858	arg1	nanogels					1870:1877	tilmicosin composite nanogels	1849:1877	tilmicosin composite nanogels against the SASCVs strain	1849:1903	The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain were 2, 1, and 1 µg/mL, respectively.
34931502	6	59	theme	optimized	1169:1177	arg1	nanogels					1200:1207	the optimized tilmicosin composite nanogels	1165:1207	the optimized tilmicosin composite nanogels	1165:1207	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	5	60	theme	optimized	974:982	arg1	formulation					984:994	The optimized formulation	970:994	The optimized formulation	970:994	RESULTS The optimized formulation was comprised of 50 mg/mL (COS), 32 mg/mL (CMC), and 0.25 mg/mL (TPP).
34931502	2	61	theme	tilmicosin	398:407	arg1	capacity					386:393	the bioadhesion capacity	370:393	the bioadhesion capacity of tilmicosin for the SASCVs strain	370:429	OBJECTIVES This study aimed to increase the bioadhesion capacity of tilmicosin for the SASCVs strain and improve the antibacterial effect of tilmicosin against cow mastitis caused by the SASCVs strain.
34931502	6	62	theme	±	1248:1248	arg1	%					1253:1253	21.9 ± 0.4%	1243:1253	21.9 ± 0.4%	1243:1253	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	3	63	theme	carboxymethyl	596:608	arg1	nanogels					636:643	Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels	540:643	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels	532:643	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers).
34931502	9	64	theme	standard	1656:1663	arg1	tilmicosin					1645:1654	tilmicosin standard	1645:1663	tilmicosin standard	1645:1663	The inhibition zone of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels were 2.13 ± 0.07, 3.35 ± 0.11, and 1.46 ± 0.04 cm, respectively.
34931502	6	65	theme	tilmicosin	1179:1188	arg1	nanogels					1200:1207	the optimized tilmicosin composite nanogels	1165:1207	the optimized tilmicosin composite nanogels	1165:1207	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	4	66	theme	composite	914:922	arg1	nanogels					924:931	tilmicosin composite nanogels	903:931	tilmicosin composite nanogels	903:931	The formation mechanism, structural characteristics, bioadhesion, and antibacterial activity of tilmicosin composite nanogels were studied systematically.
34931502	7	67	link	crosslinked	1423:1433	arg1	networks					1445:1452	nano-sized crosslinked polymeric networks	1412:1452	nano-sized crosslinked polymeric networks	1412:1452	Scanning electron microscopy showed that tilmicosin might be incorporated in nano-sized crosslinked polymeric networks.
34931502	1	68	theme	treatment	245:253	arg1	failure					255:261	treatment failure	245:261	treatment failure	245:261	BACKGROUND The poor bioadhesion capacity of tilmicosin resulting in treatment failure for Staphylococcus aureus small colony variants (SASCVs) mastitis.
34931502	9	69	theme	composite	1681:1689	arg1	nanogels					1691:1698	tilmicosin composite nanogels	1670:1698	tilmicosin composite nanogels	1670:1698	The inhibition zone of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels were 2.13 ± 0.07, 3.35 ± 0.11, and 1.46 ± 0.04 cm, respectively.
34931502	7	70	theme	Scanning	1335:1342	arg1	microscopy					1353:1362	Scanning electron microscopy	1335:1362	Scanning electron microscopy	1335:1362	Scanning electron microscopy showed that tilmicosin might be incorporated in nano-sized crosslinked polymeric networks.
34931502	6	71	theme	±	1279:1279	arg1	mV					1285:1286	-37.1 ± 0.4 mV	1273:1286	-37.1 ± 0.4 mV	1273:1286	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	6	72	theme	±	1235:1235	arg1	%					1240:1240	65.4 ± 0.4%	1230:1240	65.4 ± 0.4%	1230:1240	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	11	73	theme	tilmicosin	1992:2001	arg1	nanogels					2013:2020	the tilmicosin composite nanogels	1988:2020	the tilmicosin composite nanogels	1988:2020	The in vitro time-killing curves showed that the tilmicosin composite nanogels increased the antibacterial activity against the SASCVs strain.
34931502	0	74	theme	colony	82:87	arg1	variants					89:96	Staphylococcus aureus small colony variants	54:96	Staphylococcus aureus small colony variants	54:96	Enhanced antibacterial activity of tilmicosin against Staphylococcus aureus small colony variants by chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels.
34931502	10	75	theme	minimum	1769:1775	arg1	µg/mL					1922:1926	2, 1, and 1 µg/mL	1910:1926	2, 1, and 1 µg/mL	1910:1926	The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain were 2, 1, and 1 µg/mL, respectively.
34931502	10	75	theme	minimum	1769:1775	arg1	concentration					1788:1800	The minimum inhibitory concentration	1765:1800	The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain	1765:1903	The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain were 2, 1, and 1 µg/mL, respectively.
34931502	1	76	theme	bioadhesion	197:207	arg1	capacity					209:216	The poor bioadhesion capacity	188:216	The poor bioadhesion capacity of tilmicosin resulting in treatment failure for Staphylococcus aureus small colony variants (SASCVs) mastitis	188:327	BACKGROUND The poor bioadhesion capacity of tilmicosin resulting in treatment failure for Staphylococcus aureus small colony variants (SASCVs) mastitis.
34931502	11	77	theme	composite	2003:2011	arg1	nanogels					2013:2020	the tilmicosin composite nanogels	1988:2020	the tilmicosin composite nanogels	1988:2020	The in vitro time-killing curves showed that the tilmicosin composite nanogels increased the antibacterial activity against the SASCVs strain.
34931502	0	78	theme	oligosaccharide-sodium	110:131	arg1	nanogels					167:174	chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels	101:174	chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels	101:174	Enhanced antibacterial activity of tilmicosin against Staphylococcus aureus small colony variants by chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels.
34931502	1	79	dep	Staphylococcus	267:280	arg1	aureus					282:287	aureus	282:287	aureus	282:287	BACKGROUND The poor bioadhesion capacity of tilmicosin resulting in treatment failure for Staphylococcus aureus small colony variants (SASCVs) mastitis.
34931502	3	80	dep	METHODS	532:538	arg1	nanogels					636:643	Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels	540:643	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels	532:643	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers).
34931502	11	81	theme	time-killing	1956:1967	arg1	curves					1969:1974	The in vitro time-killing curves	1943:1974	The in vitro time-killing curves	1943:1974	The in vitro time-killing curves showed that the tilmicosin composite nanogels increased the antibacterial activity against the SASCVs strain.
34931502	12	82	theme	SASCVs	2220:2225	arg1	strain					2227:2232	the SASCVs strain	2216:2232	the SASCVs strain	2216:2232	CONCLUSIONS This study provides a potential strategy for developing tilmicosin composite nanogels to treat cow mastitis caused by the SASCVs strain.
34931502	12	83	theme	cow	2193:2195	arg1	mastitis					2197:2204	cow mastitis	2193:2204	cow mastitis caused by the SASCVs strain	2193:2232	CONCLUSIONS This study provides a potential strategy for developing tilmicosin composite nanogels to treat cow mastitis caused by the SASCVs strain.
34931502	2	84	theme	bioadhesion	374:384	arg1	capacity					386:393	the bioadhesion capacity	370:393	the bioadhesion capacity of tilmicosin for the SASCVs strain	370:429	OBJECTIVES This study aimed to increase the bioadhesion capacity of tilmicosin for the SASCVs strain and improve the antibacterial effect of tilmicosin against cow mastitis caused by the SASCVs strain.
34931502	0	85	theme	cellulose	147:155	arg1	nanogels					167:174	chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels	101:174	chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels	101:174	Enhanced antibacterial activity of tilmicosin against Staphylococcus aureus small colony variants by chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels.
34931502	8	86	theme	composite	1508:1516	arg1	nanogels					1518:1525	tilmicosin composite nanogels	1497:1525	tilmicosin composite nanogels	1497:1525	Moreover, adhesive studies suggested that tilmicosin composite nanogels could enhance the bioadhesion capacity of tilmicosin for the SASCVs strain.
34931502	9	87	dep	2.13	1705:1708	arg1	±					1710:1710	±	1710:1710	±	1710:1710	The inhibition zone of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels were 2.13 ± 0.07, 3.35 ± 0.11, and 1.46 ± 0.04 cm, respectively.
34931502	0	88	theme	antibacterial	9:21	arg1	activity					23:30	antibacterial activity	9:30	antibacterial activity of tilmicosin against Staphylococcus aureus small colony variants	9:96	Enhanced antibacterial activity of tilmicosin against Staphylococcus aureus small colony variants by chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels.
34931502	6	89	theme	encapsulation	1077:1089	arg1	efficiency					1091:1100	encapsulation efficiency	1077:1100	encapsulation efficiency	1077:1100	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	12	90	theme	composite	2165:2173	arg1	nanogels					2175:2182	tilmicosin composite nanogels	2154:2182	tilmicosin composite nanogels	2154:2182	CONCLUSIONS This study provides a potential strategy for developing tilmicosin composite nanogels to treat cow mastitis caused by the SASCVs strain.
34931502	2	91	theme	cow	490:492	arg1	mastitis					494:501	cow mastitis	490:501	cow mastitis caused by the SASCVs strain	490:529	OBJECTIVES This study aimed to increase the bioadhesion capacity of tilmicosin for the SASCVs strain and improve the antibacterial effect of tilmicosin against cow mastitis caused by the SASCVs strain.
34931502	2	92	theme	tilmicosin	471:480	arg1	effect					461:466	the antibacterial effect	443:466	the antibacterial effect of tilmicosin against cow mastitis caused by the SASCVs strain	443:529	OBJECTIVES This study aimed to increase the bioadhesion capacity of tilmicosin for the SASCVs strain and improve the antibacterial effect of tilmicosin against cow mastitis caused by the SASCVs strain.
34931502	3	93	theme	positive	706:713	arg1	COS					701:703	COS	701:703	COS (positive charge)	701:721	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers).
34931502	3	93	theme	positive	706:713	arg1	charge					715:720	positive charge	706:720	positive charge	706:720	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers).
34931502	0	94	theme	aureus	69:74	arg1	variants					89:96	Staphylococcus aureus small colony variants	54:96	Staphylococcus aureus small colony variants	54:96	Enhanced antibacterial activity of tilmicosin against Staphylococcus aureus small colony variants by chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels.
34931502	6	95	theme	zeta	1147:1150	arg1	potential					1152:1160	zeta potential	1147:1160	zeta potential	1147:1160	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	2	96	theme	SASCVs	417:422	arg1	strain					424:429	the SASCVs strain	413:429	the SASCVs strain	413:429	OBJECTIVES This study aimed to increase the bioadhesion capacity of tilmicosin for the SASCVs strain and improve the antibacterial effect of tilmicosin against cow mastitis caused by the SASCVs strain.
34931502	8	97	theme	tilmicosin	1569:1578	arg1	capacity					1557:1564	the bioadhesion capacity	1541:1564	the bioadhesion capacity of tilmicosin for the SASCVs strain	1541:1600	Moreover, adhesive studies suggested that tilmicosin composite nanogels could enhance the bioadhesion capacity of tilmicosin for the SASCVs strain.
34931502	3	98	theme	negative	732:739	arg1	CMC					727:729	CMC	727:729	CMC (negative charge)	727:747	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers).
34931502	3	98	theme	negative	732:739	arg1	charge					741:746	negative charge	732:746	negative charge	732:746	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers).
34931502	4	99	theme	antibacterial	877:889	arg1	activity					891:898	antibacterial activity	877:898	antibacterial activity	877:898	The formation mechanism, structural characteristics, bioadhesion, and antibacterial activity of tilmicosin composite nanogels were studied systematically.
34931502	3	100	theme	oligosaccharide	567:581	arg1	nanogels					636:643	Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels	540:643	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels	532:643	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers).
34931502	10	101	theme	standard	1835:1842	arg1	tilmicosin					1824:1833	tilmicosin standard	1824:1842	tilmicosin standard	1824:1842	The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain were 2, 1, and 1 µg/mL, respectively.
34931502	4	102	theme	structural	832:841	arg1	characteristics					843:857	structural characteristics	832:857	structural characteristics	832:857	The formation mechanism, structural characteristics, bioadhesion, and antibacterial activity of tilmicosin composite nanogels were studied systematically.
34931502	3	103	theme	Tilmicosin-loaded	540:556	arg1	nanogels					636:643	Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels	540:643	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels	532:643	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers).
34931502	1	104	theme	small	289:293	arg1	mastitis					320:327	Staphylococcus aureus small colony variants (SASCVs) mastitis	267:327	Staphylococcus aureus small colony variants (SASCVs) mastitis	267:327	BACKGROUND The poor bioadhesion capacity of tilmicosin resulting in treatment failure for Staphylococcus aureus small colony variants (SASCVs) mastitis.
34931502	9	105	theme	inhibition	1607:1616	arg1	2.13					1705:1708	2.13	1705:1708	2.13	1705:1708	The inhibition zone of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels were 2.13 ± 0.07, 3.35 ± 0.11, and 1.46 ± 0.04 cm, respectively.
34931502	9	105	theme	inhibition	1607:1616	arg1	zone					1618:1621	The inhibition zone	1603:1621	The inhibition zone of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels	1603:1698	The inhibition zone of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels were 2.13 ± 0.07, 3.35 ± 0.11, and 1.46 ± 0.04 cm, respectively.
34931502	6	106	theme	polydispersity	1121:1134	arg1	index					1136:1140	polydispersity index	1121:1140	polydispersity index	1121:1140	The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one.
34931502	3	107	theme	sodium	755:760	arg1	TPP					780:782	TPP	780:782	TPP	780:782	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers).
34931502	3	107	theme	sodium	755:760	arg1	tripolyphosphate					762:777	sodium tripolyphosphate	755:777	sodium tripolyphosphate (TPP) (ionic crosslinkers)	755:804	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers).
34931502	3	107	theme	sodium	755:760	arg1	crosslinkers					792:803	ionic crosslinkers	786:803	ionic crosslinkers	786:803	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers).
34931502	3	108	theme	-sodium	588:594	arg1	nanogels					636:643	Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels	540:643	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels	532:643	METHODS Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers).
34931502	1	109	theme	variants	302:309	arg1	mastitis					320:327	Staphylococcus aureus small colony variants (SASCVs) mastitis	267:327	Staphylococcus aureus small colony variants (SASCVs) mastitis	267:327	BACKGROUND The poor bioadhesion capacity of tilmicosin resulting in treatment failure for Staphylococcus aureus small colony variants (SASCVs) mastitis.
33092885	0	0	with	composites	52:61	arg1	structure					81:89	controllable structure	68:89	controllable structure	68:89	Film-like bacterial cellulose/cyclodextrin oligomer composites with controllable structure for the removal of various persistent organic pollutants from water.
33092885	4	1	theme	optimum	668:674	arg1	product					676:682	The optimum product	664:682	The optimum product	664:682	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	3	2	theme	cellulose	653:661	arg1	nanofibers					626:635	the ultrafine nanofibers	612:635	the ultrafine nanofibers of 3D bacterial cellulose	612:661	In this study, we describe a strategy for film-like water purifier, prepared by loading cyclodextrin (CD) oligomer onto the ultrafine nanofibers of 3D bacterial cellulose.
33092885	1	3	theme	persistent	186:195	arg1	POPs					217:220	POPs	217:220	POPs	217:220	The adsorptive removal of persistent organic pollutants (POPs) is reckoned as a simple, convenient, and practical technology, especially in decentralized systems and remote areas.
33092885	1	3	theme	persistent	186:195	arg1	pollutants					205:214	persistent organic pollutants	186:214	persistent organic pollutants (POPs)	186:221	The adsorptive removal of persistent organic pollutants (POPs) is reckoned as a simple, convenient, and practical technology, especially in decentralized systems and remote areas.
33092885	5	4	theme	excellent	1082:1090	arg1	reusability					1092:1102	excellent reusability	1082:1102	excellent reusability	1082:1102	Moreover, our sample demonstrated stable adsorption ability over a broad pH range and under more complex water conditions, and more importantly excellent reusability.
33092885	8	5	theme	excellent	1415:1423	arg1	performance					1425:1435	excellent performance	1415:1435	excellent performance	1415:1435	Furthermore, the product can be used to treat actual sewage with its convenient film-like shape and excellent performance, which improves its potential in complex systems and large-scale applications.
33092885	0	6	theme	persistent	118:127	arg1	pollutants					137:146	various persistent organic pollutants	110:146	various persistent organic pollutants from water	110:157	Film-like bacterial cellulose/cyclodextrin oligomer composites with controllable structure for the removal of various persistent organic pollutants from water.
33092885	8	7	theme	convenient	1384:1393	arg1	shape					1405:1409	its convenient film-like shape	1380:1409	its convenient film-like shape	1380:1409	Furthermore, the product can be used to treat actual sewage with its convenient film-like shape and excellent performance, which improves its potential in complex systems and large-scale applications.
33092885	4	8	theme	various	727:733	arg1	A					779:779	bisphenol A	769:779	bisphenol A (BPA)	769:785	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	4	8	theme	various	727:733	arg1	phenol					761:766	phenol	761:766	phenol	761:766	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	4	8	theme	various	727:733	arg1	2,4-Dichlorophenol					803:820	2,4-Dichlorophenol	803:820	2,4-Dichlorophenol (2,4-DCP)	803:830	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	4	8	theme	various	727:733	arg1	pollutants					742:751	various target pollutants	727:751	various target pollutants such as phenol	727:766	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	4	8	theme	various	727:733	arg1	glyphosate					788:797	glyphosate	788:797	glyphosate	788:797	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	1	9	theme	practical	264:272	arg1	removal					175:181	The adsorptive removal	160:181	The adsorptive removal of persistent organic pollutants (POPs)	160:221	The adsorptive removal of persistent organic pollutants (POPs) is reckoned as a simple, convenient, and practical technology, especially in decentralized systems and remote areas.
33092885	1	9	theme	practical	264:272	arg1	technology					274:283	a simple, convenient, and practical technology	238:283	a simple, convenient, and practical technology	238:283	The adsorptive removal of persistent organic pollutants (POPs) is reckoned as a simple, convenient, and practical technology, especially in decentralized systems and remote areas.
33092885	0	10	theme	various	110:116	arg1	pollutants					137:146	various persistent organic pollutants	110:146	various persistent organic pollutants from water	110:157	Film-like bacterial cellulose/cyclodextrin oligomer composites with controllable structure for the removal of various persistent organic pollutants from water.
33092885	7	11	with	design	1227:1232	arg1	ability					1279:1285	high yet stable adsorption ability	1252:1285	high yet stable adsorption ability	1252:1285	We reckon our study provides new insight for the design of adsorbent with high yet stable adsorption ability and controllable structure.
33092885	7	11	with	design	1227:1232	arg1	structure					1304:1312	controllable structure	1291:1312	controllable structure	1291:1312	We reckon our study provides new insight for the design of adsorbent with high yet stable adsorption ability and controllable structure.
33092885	4	12	theme	bisphenol	769:777	arg1	A					779:779	bisphenol A	769:779	bisphenol A (BPA)	769:785	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	4	12	theme	bisphenol	769:777	arg1	pollutants					742:751	various target pollutants	727:751	various target pollutants such as phenol	727:766	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	4	12	theme	bisphenol	769:777	arg1	BPA					782:784	BPA	782:784	BPA	782:784	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	4	12	theme	bisphenol	769:777	arg1	phenol					761:766	phenol	761:766	phenol	761:766	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	7	13	theme	adsorbent	1237:1245	arg1	design					1227:1232	the design	1223:1232	the design of adsorbent with high yet stable adsorption ability and controllable structure	1223:1312	We reckon our study provides new insight for the design of adsorbent with high yet stable adsorption ability and controllable structure.
33092885	0	14	theme	pollutants	137:146	arg1	removal					99:105	the removal	95:105	the removal of various persistent organic pollutants from water	95:157	Film-like bacterial cellulose/cyclodextrin oligomer composites with controllable structure for the removal of various persistent organic pollutants from water.
33092885	8	15	theme	large-scale	1490:1500	arg1	applications					1502:1513	large-scale applications	1490:1513	large-scale applications	1490:1513	Furthermore, the product can be used to treat actual sewage with its convenient film-like shape and excellent performance, which improves its potential in complex systems and large-scale applications.
33092885	1	16	theme	organic	197:203	arg1	POPs					217:220	POPs	217:220	POPs	217:220	The adsorptive removal of persistent organic pollutants (POPs) is reckoned as a simple, convenient, and practical technology, especially in decentralized systems and remote areas.
33092885	1	16	theme	organic	197:203	arg1	pollutants					205:214	persistent organic pollutants	186:214	persistent organic pollutants (POPs)	186:221	The adsorptive removal of persistent organic pollutants (POPs) is reckoned as a simple, convenient, and practical technology, especially in decentralized systems and remote areas.
33092885	0	17	theme	organic	129:135	arg1	pollutants					137:146	various persistent organic pollutants	110:146	various persistent organic pollutants from water	110:157	Film-like bacterial cellulose/cyclodextrin oligomer composites with controllable structure for the removal of various persistent organic pollutants from water.
33092885	6	18	theme	commercial	1142:1151	arg1	potential					1153:1161	the commercial potential	1138:1161	the commercial potential of our sample	1138:1175	A rough cost analysis highlights the commercial potential of our sample.
33092885	1	19	theme	pollutants	205:214	arg1	removal					175:181	The adsorptive removal	160:181	The adsorptive removal of persistent organic pollutants (POPs)	160:221	The adsorptive removal of persistent organic pollutants (POPs) is reckoned as a simple, convenient, and practical technology, especially in decentralized systems and remote areas.
33092885	1	19	theme	pollutants	205:214	arg1	technology					274:283	a simple, convenient, and practical technology	238:283	a simple, convenient, and practical technology	238:283	The adsorptive removal of persistent organic pollutants (POPs) is reckoned as a simple, convenient, and practical technology, especially in decentralized systems and remote areas.
33092885	0	20	theme	bacterial	10:18	arg1	composites					52:61	Film-like bacterial cellulose/cyclodextrin oligomer composites	0:61	Film-like bacterial cellulose/cyclodextrin oligomer composites with controllable structure for the removal of various persistent organic pollutants from water.	0:158	Film-like bacterial cellulose/cyclodextrin oligomer composites with controllable structure for the removal of various persistent organic pollutants from water.
33092885	4	21	with	pollutants	742:751	arg1	capacities					838:847	capacities	838:847	capacities higher than most adsorbents including porous carbon based materials reported previously	838:935	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	3	22	theme	water	544:548	arg1	purifier					550:557	film-like water purifier	534:557	film-like water purifier	534:557	In this study, we describe a strategy for film-like water purifier, prepared by loading cyclodextrin (CD) oligomer onto the ultrafine nanofibers of 3D bacterial cellulose.
33092885	0	23	theme	Film-like	0:8	arg1	composites					52:61	Film-like bacterial cellulose/cyclodextrin oligomer composites	0:61	Film-like bacterial cellulose/cyclodextrin oligomer composites with controllable structure for the removal of various persistent organic pollutants from water.	0:158	Film-like bacterial cellulose/cyclodextrin oligomer composites with controllable structure for the removal of various persistent organic pollutants from water.
33092885	3	24	theme	ultrafine	616:624	arg1	nanofibers					626:635	the ultrafine nanofibers	612:635	the ultrafine nanofibers of 3D bacterial cellulose	612:661	In this study, we describe a strategy for film-like water purifier, prepared by loading cyclodextrin (CD) oligomer onto the ultrafine nanofibers of 3D bacterial cellulose.
33092885	0	25	theme	oligomer	43:50	arg1	composites					52:61	Film-like bacterial cellulose/cyclodextrin oligomer composites	0:61	Film-like bacterial cellulose/cyclodextrin oligomer composites with controllable structure for the removal of various persistent organic pollutants from water.	0:158	Film-like bacterial cellulose/cyclodextrin oligomer composites with controllable structure for the removal of various persistent organic pollutants from water.
33092885	5	26	theme	adsorption	979:988	arg1	ability					990:996	stable adsorption ability	972:996	stable adsorption ability	972:996	Moreover, our sample demonstrated stable adsorption ability over a broad pH range and under more complex water conditions, and more importantly excellent reusability.
33092885	4	27	theme	porous	887:892	arg1	materials					907:915	porous carbon based materials	887:915	porous carbon based materials reported previously	887:935	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	4	28	theme	target	735:740	arg1	A					779:779	bisphenol A	769:779	bisphenol A (BPA)	769:785	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	4	28	theme	target	735:740	arg1	phenol					761:766	phenol	761:766	phenol	761:766	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	4	28	theme	target	735:740	arg1	2,4-Dichlorophenol					803:820	2,4-Dichlorophenol	803:820	2,4-Dichlorophenol (2,4-DCP)	803:830	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	4	28	theme	target	735:740	arg1	pollutants					742:751	various target pollutants	727:751	various target pollutants such as phenol	727:766	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	4	28	theme	target	735:740	arg1	glyphosate					788:797	glyphosate	788:797	glyphosate	788:797	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	8	29	theme	complex	1470:1476	arg1	systems					1478:1484	complex systems	1470:1484	complex systems	1470:1484	Furthermore, the product can be used to treat actual sewage with its convenient film-like shape and excellent performance, which improves its potential in complex systems and large-scale applications.
33092885	7	30	theme	adsorption	1268:1277	arg1	ability					1279:1285	high yet stable adsorption ability	1252:1285	high yet stable adsorption ability	1252:1285	We reckon our study provides new insight for the design of adsorbent with high yet stable adsorption ability and controllable structure.
33092885	0	31	theme	cellulose/cyclodextrin	20:41	arg1	composites					52:61	Film-like bacterial cellulose/cyclodextrin oligomer composites	0:61	Film-like bacterial cellulose/cyclodextrin oligomer composites with controllable structure for the removal of various persistent organic pollutants from water.	0:158	Film-like bacterial cellulose/cyclodextrin oligomer composites with controllable structure for the removal of various persistent organic pollutants from water.
33092885	7	32	dep	study	1192:1196	arg1	We					1178:1179	We	1178:1179	We	1178:1179	We reckon our study provides new insight for the design of adsorbent with high yet stable adsorption ability and controllable structure.
33092885	5	33	theme	complex	1035:1041	arg1	conditions					1049:1058	more complex water conditions	1030:1058	more complex water conditions	1030:1058	Moreover, our sample demonstrated stable adsorption ability over a broad pH range and under more complex water conditions, and more importantly excellent reusability.
33092885	3	34	theme	film-like	534:542	arg1	purifier					550:557	film-like water purifier	534:557	film-like water purifier	534:557	In this study, we describe a strategy for film-like water purifier, prepared by loading cyclodextrin (CD) oligomer onto the ultrafine nanofibers of 3D bacterial cellulose.
33092885	2	35	theme	controllable	405:416	arg1	structure					418:426	controllable structure	405:426	controllable structure	405:426	For this purpose, it is important to design new adsorbents, with controllable structure and convenient shape, for the highly efficient removal of POPs.
33092885	2	36	theme	efficient	465:473	arg1	removal					475:481	the highly efficient removal	454:481	the highly efficient removal of POPs	454:489	For this purpose, it is important to design new adsorbents, with controllable structure and convenient shape, for the highly efficient removal of POPs.
33092885	1	37	theme	decentralized	300:312	arg1	systems					314:320	decentralized systems	300:320	decentralized systems	300:320	The adsorptive removal of persistent organic pollutants (POPs) is reckoned as a simple, convenient, and practical technology, especially in decentralized systems and remote areas.
33092885	4	38	theme	based	901:905	arg1	materials					907:915	porous carbon based materials	887:915	porous carbon based materials reported previously	887:935	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	0	39	from	removal	99:105	arg1	water					153:157	water	153:157	water	153:157	Film-like bacterial cellulose/cyclodextrin oligomer composites with controllable structure for the removal of various persistent organic pollutants from water.
33092885	8	40	theme	actual	1361:1366	arg1	sewage					1368:1373	actual sewage	1361:1373	actual sewage with its convenient film-like shape and excellent performance, which improves its potential in complex systems and large-scale applications	1361:1513	Furthermore, the product can be used to treat actual sewage with its convenient film-like shape and excellent performance, which improves its potential in complex systems and large-scale applications.
33092885	7	41	theme	controllable	1291:1302	arg1	structure					1304:1312	controllable structure	1291:1312	controllable structure	1291:1312	We reckon our study provides new insight for the design of adsorbent with high yet stable adsorption ability and controllable structure.
33092885	4	42	theme	carbon	894:899	arg1	materials					907:915	porous carbon based materials	887:915	porous carbon based materials reported previously	887:935	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	2	43	theme	convenient	432:441	arg1	shape					443:447	convenient shape	432:447	convenient shape	432:447	For this purpose, it is important to design new adsorbents, with controllable structure and convenient shape, for the highly efficient removal of POPs.
33092885	7	44	theme	stable	1261:1266	arg1	ability					1279:1285	high yet stable adsorption ability	1252:1285	high yet stable adsorption ability	1252:1285	We reckon our study provides new insight for the design of adsorbent with high yet stable adsorption ability and controllable structure.
33092885	8	45	from	potential	1457:1465	arg1	systems					1478:1484	complex systems	1470:1484	complex systems	1470:1484	Furthermore, the product can be used to treat actual sewage with its convenient film-like shape and excellent performance, which improves its potential in complex systems and large-scale applications.
33092885	8	45	from	potential	1457:1465	arg1	applications					1502:1513	large-scale applications	1490:1513	large-scale applications	1490:1513	Furthermore, the product can be used to treat actual sewage with its convenient film-like shape and excellent performance, which improves its potential in complex systems and large-scale applications.
33092885	7	46	theme	new	1207:1209	arg1	insight					1211:1217	new insight	1207:1217	new insight for the design of adsorbent with high yet stable adsorption ability and controllable structure	1207:1312	We reckon our study provides new insight for the design of adsorbent with high yet stable adsorption ability and controllable structure.
33092885	5	47	theme	broad	1005:1009	arg1	range					1014:1018	a broad pH range	1003:1018	a broad pH range	1003:1018	Moreover, our sample demonstrated stable adsorption ability over a broad pH range and under more complex water conditions, and more importantly excellent reusability.
33092885	6	48	theme	rough	1107:1111	arg1	analysis					1118:1125	A rough cost analysis	1105:1125	A rough cost analysis	1105:1125	A rough cost analysis highlights the commercial potential of our sample.
33092885	0	49	theme	controllable	68:79	arg1	structure					81:89	controllable structure	68:89	controllable structure	68:89	Film-like bacterial cellulose/cyclodextrin oligomer composites with controllable structure for the removal of various persistent organic pollutants from water.
33092885	1	50	theme	remote	326:331	arg1	areas					333:337	remote areas	326:337	remote areas	326:337	The adsorptive removal of persistent organic pollutants (POPs) is reckoned as a simple, convenient, and practical technology, especially in decentralized systems and remote areas.
33092885	4	51	theme	higher	849:854	arg1	capacities					838:847	capacities	838:847	capacities higher than most adsorbents including porous carbon based materials reported previously	838:935	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	3	52	theme	3D	640:641	arg1	cellulose					653:661	3D bacterial cellulose	640:661	3D bacterial cellulose	640:661	In this study, we describe a strategy for film-like water purifier, prepared by loading cyclodextrin (CD) oligomer onto the ultrafine nanofibers of 3D bacterial cellulose.
33092885	5	53	theme	pH	1011:1012	arg1	range					1014:1018	a broad pH range	1003:1018	a broad pH range	1003:1018	Moreover, our sample demonstrated stable adsorption ability over a broad pH range and under more complex water conditions, and more importantly excellent reusability.
33092885	7	54	theme	reckon	1181:1186	arg1	study					1192:1196	We reckon our study	1178:1196	We reckon our study	1178:1196	We reckon our study provides new insight for the design of adsorbent with high yet stable adsorption ability and controllable structure.
33092885	7	55	theme	high	1252:1255	arg1	ability					1279:1285	high yet stable adsorption ability	1252:1285	high yet stable adsorption ability	1252:1285	We reckon our study provides new insight for the design of adsorbent with high yet stable adsorption ability and controllable structure.
33092885	8	56	theme	film-like	1395:1403	arg1	shape					1405:1409	its convenient film-like shape	1380:1409	its convenient film-like shape	1380:1409	Furthermore, the product can be used to treat actual sewage with its convenient film-like shape and excellent performance, which improves its potential in complex systems and large-scale applications.
33092885	3	57	theme	bacterial	643:651	arg1	cellulose					653:661	3D bacterial cellulose	640:661	3D bacterial cellulose	640:661	In this study, we describe a strategy for film-like water purifier, prepared by loading cyclodextrin (CD) oligomer onto the ultrafine nanofibers of 3D bacterial cellulose.
33092885	3	58	theme	CD	594:595	arg1	oligomer					598:605	loading cyclodextrin (CD) oligomer	572:605	loading cyclodextrin (CD) oligomer onto the ultrafine nanofibers of 3D bacterial cellulose	572:661	In this study, we describe a strategy for film-like water purifier, prepared by loading cyclodextrin (CD) oligomer onto the ultrafine nanofibers of 3D bacterial cellulose.
33092885	3	59	theme	loading	572:578	arg1	oligomer					598:605	loading cyclodextrin (CD) oligomer	572:605	loading cyclodextrin (CD) oligomer onto the ultrafine nanofibers of 3D bacterial cellulose	572:661	In this study, we describe a strategy for film-like water purifier, prepared by loading cyclodextrin (CD) oligomer onto the ultrafine nanofibers of 3D bacterial cellulose.
33092885	4	60	theme	removal	704:710	arg1	ability					712:718	remarkable removal ability	693:718	remarkable removal ability	693:718	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	4	61	theme	most	861:864	arg1	materials					907:915	porous carbon based materials	887:915	porous carbon based materials reported previously	887:935	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	4	61	theme	most	861:864	arg1	adsorbents					866:875	most adsorbents	861:875	most adsorbents including porous carbon based materials reported previously	861:935	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	2	62	with	important	364:372	arg1	shape					443:447	convenient shape	432:447	convenient shape	432:447	For this purpose, it is important to design new adsorbents, with controllable structure and convenient shape, for the highly efficient removal of POPs.
33092885	2	62	with	important	364:372	arg1	structure					418:426	controllable structure	405:426	controllable structure	405:426	For this purpose, it is important to design new adsorbents, with controllable structure and convenient shape, for the highly efficient removal of POPs.
33092885	5	63	theme	stable	972:977	arg1	ability					990:996	stable adsorption ability	972:996	stable adsorption ability	972:996	Moreover, our sample demonstrated stable adsorption ability over a broad pH range and under more complex water conditions, and more importantly excellent reusability.
33092885	5	64	theme	water	1043:1047	arg1	conditions					1049:1058	more complex water conditions	1030:1058	more complex water conditions	1030:1058	Moreover, our sample demonstrated stable adsorption ability over a broad pH range and under more complex water conditions, and more importantly excellent reusability.
33092885	4	65	theme	remarkable	693:702	arg1	ability					712:718	remarkable removal ability	693:718	remarkable removal ability	693:718	The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously.
33092885	1	66	theme	simple	240:245	arg1	removal					175:181	The adsorptive removal	160:181	The adsorptive removal of persistent organic pollutants (POPs)	160:221	The adsorptive removal of persistent organic pollutants (POPs) is reckoned as a simple, convenient, and practical technology, especially in decentralized systems and remote areas.
33092885	1	66	theme	simple	240:245	arg1	technology					274:283	a simple, convenient, and practical technology	238:283	a simple, convenient, and practical technology	238:283	The adsorptive removal of persistent organic pollutants (POPs) is reckoned as a simple, convenient, and practical technology, especially in decentralized systems and remote areas.
33092885	3	67	theme	cyclodextrin	580:591	arg1	oligomer					598:605	loading cyclodextrin (CD) oligomer	572:605	loading cyclodextrin (CD) oligomer onto the ultrafine nanofibers of 3D bacterial cellulose	572:661	In this study, we describe a strategy for film-like water purifier, prepared by loading cyclodextrin (CD) oligomer onto the ultrafine nanofibers of 3D bacterial cellulose.
33092885	8	68	used	used	1347:1350	arg2	product					1332:1338	the product	1328:1338	the product	1328:1338	Furthermore, the product can be used to treat actual sewage with its convenient film-like shape and excellent performance, which improves its potential in complex systems and large-scale applications.
33092885	1	69	theme	adsorptive	164:173	arg1	removal					175:181	The adsorptive removal	160:181	The adsorptive removal of persistent organic pollutants (POPs)	160:221	The adsorptive removal of persistent organic pollutants (POPs) is reckoned as a simple, convenient, and practical technology, especially in decentralized systems and remote areas.
33092885	1	69	theme	adsorptive	164:173	arg1	technology					274:283	a simple, convenient, and practical technology	238:283	a simple, convenient, and practical technology	238:283	The adsorptive removal of persistent organic pollutants (POPs) is reckoned as a simple, convenient, and practical technology, especially in decentralized systems and remote areas.
33092885	2	70	theme	new	384:386	arg1	adsorbents					388:397	new adsorbents	384:397	new adsorbents	384:397	For this purpose, it is important to design new adsorbents, with controllable structure and convenient shape, for the highly efficient removal of POPs.
33092885	0	71	from	water	153:157	arg1	removal					99:105	the removal	95:105	the removal of various persistent organic pollutants from water	95:157	Film-like bacterial cellulose/cyclodextrin oligomer composites with controllable structure for the removal of various persistent organic pollutants from water.
33092885	0	71	from	water	153:157	arg1	pollutants					137:146	various persistent organic pollutants	110:146	various persistent organic pollutants from water	110:157	Film-like bacterial cellulose/cyclodextrin oligomer composites with controllable structure for the removal of various persistent organic pollutants from water.
33092885	2	72	theme	POPs	486:489	arg1	removal					475:481	the highly efficient removal	454:481	the highly efficient removal of POPs	454:489	For this purpose, it is important to design new adsorbents, with controllable structure and convenient shape, for the highly efficient removal of POPs.
33092885	6	73	theme	cost	1113:1116	arg1	analysis					1118:1125	A rough cost analysis	1105:1125	A rough cost analysis	1105:1125	A rough cost analysis highlights the commercial potential of our sample.
33092885	6	74	theme	sample	1170:1175	arg1	potential					1153:1161	the commercial potential	1138:1161	the commercial potential of our sample	1138:1175	A rough cost analysis highlights the commercial potential of our sample.
33092885	8	75	with	sewage	1368:1373	arg1	performance					1425:1435	excellent performance	1415:1435	excellent performance	1415:1435	Furthermore, the product can be used to treat actual sewage with its convenient film-like shape and excellent performance, which improves its potential in complex systems and large-scale applications.
33092885	8	75	with	sewage	1368:1373	arg1	shape					1405:1409	its convenient film-like shape	1380:1409	its convenient film-like shape	1380:1409	Furthermore, the product can be used to treat actual sewage with its convenient film-like shape and excellent performance, which improves its potential in complex systems and large-scale applications.
33092885	1	76	theme	convenient	248:257	arg1	removal					175:181	The adsorptive removal	160:181	The adsorptive removal of persistent organic pollutants (POPs)	160:221	The adsorptive removal of persistent organic pollutants (POPs) is reckoned as a simple, convenient, and practical technology, especially in decentralized systems and remote areas.
33092885	1	76	theme	convenient	248:257	arg1	technology					274:283	a simple, convenient, and practical technology	238:283	a simple, convenient, and practical technology	238:283	The adsorptive removal of persistent organic pollutants (POPs) is reckoned as a simple, convenient, and practical technology, especially in decentralized systems and remote areas.
33357920	5	0	theme	thermogravimetric	996:1012	arg1	results					1029:1035	the thermogravimetric analysis (TGA) results	992:1035	the thermogravimetric analysis (TGA) results	992:1035	Furthermore, the incorporation of the CA nanofiber mats also significantly improved the flame retardancy of the HPLs, which was revealed from the thermogravimetric analysis (TGA) results.
33357920	2	1	theme	average	407:413	arg1	diameter					415:422	an average diameter	404:422	an average diameter of 654±246 nm	404:436	When the concentration of CA was 16 wt%, SEM images demonstrated that the morphology of the CA nanofiber mat was the best, with an average diameter of 654±246 nm.
33357920	5	2	theme	analysis	1014:1021	arg1	results					1029:1035	the thermogravimetric analysis (TGA) results	992:1035	the thermogravimetric analysis (TGA) results	992:1035	Furthermore, the incorporation of the CA nanofiber mats also significantly improved the flame retardancy of the HPLs, which was revealed from the thermogravimetric analysis (TGA) results.
33357920	4	3	theme	tensile	614:620	arg1	strength					622:629	tensile strength	614:629	tensile strength	614:629	Specifically, the tensile strength and elongation at break of the nanofiber mats reinforced HPLs composites increased remarkably to 40.8 ±1.1 MPa and 27.9 ± 0.9 %, respectively, which were nearly 6 times and 4.4 times higher than those of the pure HPLs.
33357920	4	4	theme	HPLs	688:691	arg1	composites					693:702	the nanofiber mats reinforced HPLs composites	658:702	the nanofiber mats reinforced HPLs composites	658:702	Specifically, the tensile strength and elongation at break of the nanofiber mats reinforced HPLs composites increased remarkably to 40.8 ±1.1 MPa and 27.9 ± 0.9 %, respectively, which were nearly 6 times and 4.4 times higher than those of the pure HPLs.
33357920	4	5	theme	nanofiber	662:670	arg1	composites					693:702	the nanofiber mats reinforced HPLs composites	658:702	the nanofiber mats reinforced HPLs composites	658:702	Specifically, the tensile strength and elongation at break of the nanofiber mats reinforced HPLs composites increased remarkably to 40.8 ±1.1 MPa and 27.9 ± 0.9 %, respectively, which were nearly 6 times and 4.4 times higher than those of the pure HPLs.
33357920	4	6	dep	increased	704:712	arg1	times					794:798	6 times	792:798	6 times	792:798	Specifically, the tensile strength and elongation at break of the nanofiber mats reinforced HPLs composites increased remarkably to 40.8 ±1.1 MPa and 27.9 ± 0.9 %, respectively, which were nearly 6 times and 4.4 times higher than those of the pure HPLs.
33357920	4	6	dep	increased	704:712	arg1	times					808:812	4.4 times	804:812	4.4 times	804:812	Specifically, the tensile strength and elongation at break of the nanofiber mats reinforced HPLs composites increased remarkably to 40.8 ±1.1 MPa and 27.9 ± 0.9 %, respectively, which were nearly 6 times and 4.4 times higher than those of the pure HPLs.
33357920	4	7	theme	reinforced	677:686	arg1	composites					693:702	the nanofiber mats reinforced HPLs composites	658:702	the nanofiber mats reinforced HPLs composites	658:702	Specifically, the tensile strength and elongation at break of the nanofiber mats reinforced HPLs composites increased remarkably to 40.8 ±1.1 MPa and 27.9 ± 0.9 %, respectively, which were nearly 6 times and 4.4 times higher than those of the pure HPLs.
33357920	1	8	theme	high-pressure	244:256	arg1	HPLs					269:272	HPLs	269:272	HPLs	269:272	In the work, the non-woven cellulose acetate (CA) nanofiber mats were prepared via electrospinning, and CA nanofiber were incorporated into the core layer of the high-pressure laminates (HPLs).
33357920	1	8	theme	high-pressure	244:256	arg1	laminates					258:266	the high-pressure laminates	240:266	the high-pressure laminates (HPLs)	240:273	In the work, the non-woven cellulose acetate (CA) nanofiber mats were prepared via electrospinning, and CA nanofiber were incorporated into the core layer of the high-pressure laminates (HPLs).
33357920	1	9	theme	cellulose	109:117	arg1	CA					128:129	CA	128:129	CA	128:129	In the work, the non-woven cellulose acetate (CA) nanofiber mats were prepared via electrospinning, and CA nanofiber were incorporated into the core layer of the high-pressure laminates (HPLs).
33357920	1	9	theme	cellulose	109:117	arg1	acetate					119:125	non-woven cellulose acetate	99:125	the non-woven cellulose acetate (CA) nanofiber mats	95:145	In the work, the non-woven cellulose acetate (CA) nanofiber mats were prepared via electrospinning, and CA nanofiber were incorporated into the core layer of the high-pressure laminates (HPLs).
33357920	3	10	theme	nanofiber	447:455	arg1	mats					457:460	CA nanofiber mats	444:460	CA nanofiber mats	444:460	When CA nanofiber mats were incorporated into the core layer of HPLs, the mechanical properties of the resulted HPLs composites were significantly improved.
33357920	2	11	theme	nm	435:436	arg1	diameter					415:422	an average diameter	404:422	an average diameter of 654±246 nm	404:436	When the concentration of CA was 16 wt%, SEM images demonstrated that the morphology of the CA nanofiber mat was the best, with an average diameter of 654±246 nm.
33357920	4	12	theme	composites	693:702	arg1	elongation					635:644	elongation	635:644	elongation	635:644	Specifically, the tensile strength and elongation at break of the nanofiber mats reinforced HPLs composites increased remarkably to 40.8 ±1.1 MPa and 27.9 ± 0.9 %, respectively, which were nearly 6 times and 4.4 times higher than those of the pure HPLs.
33357920	4	12	theme	composites	693:702	arg1	strength					622:629	tensile strength	614:629	tensile strength	614:629	Specifically, the tensile strength and elongation at break of the nanofiber mats reinforced HPLs composites increased remarkably to 40.8 ±1.1 MPa and 27.9 ± 0.9 %, respectively, which were nearly 6 times and 4.4 times higher than those of the pure HPLs.
33357920	5	13	theme	HPLs	962:965	arg1	retardancy					944:953	the flame retardancy	934:953	the flame retardancy of the HPLs, which was revealed from the thermogravimetric analysis (TGA) results	934:1035	Furthermore, the incorporation of the CA nanofiber mats also significantly improved the flame retardancy of the HPLs, which was revealed from the thermogravimetric analysis (TGA) results.
33357920	3	14	theme	core	489:492	arg1	layer					494:498	the core layer	485:498	the core layer of HPLs	485:506	When CA nanofiber mats were incorporated into the core layer of HPLs, the mechanical properties of the resulted HPLs composites were significantly improved.
33357920	0	15	theme	High	0:3	arg1	pressure					5:12	High pressure	0:12	High pressure	0:12	High pressure laminates reinforced with electrospun cellulose acetate nanofibers.
33357920	1	16	theme	laminates	258:266	arg1	layer					231:235	the core layer	222:235	the core layer of the high-pressure laminates (HPLs)	222:273	In the work, the non-woven cellulose acetate (CA) nanofiber mats were prepared via electrospinning, and CA nanofiber were incorporated into the core layer of the high-pressure laminates (HPLs).
33357920	2	17	theme	mat	381:383	arg1	best					393:396	best	393:396	best	393:396	When the concentration of CA was 16 wt%, SEM images demonstrated that the morphology of the CA nanofiber mat was the best, with an average diameter of 654±246 nm.
33357920	2	17	theme	mat	381:383	arg1	morphology					350:359	the morphology	346:359	the morphology of the CA nanofiber mat	346:383	When the concentration of CA was 16 wt%, SEM images demonstrated that the morphology of the CA nanofiber mat was the best, with an average diameter of 654±246 nm.
33357920	4	18	theme	higher	814:819	arg1	times					794:798	6 times	792:798	6 times	792:798	Specifically, the tensile strength and elongation at break of the nanofiber mats reinforced HPLs composites increased remarkably to 40.8 ±1.1 MPa and 27.9 ± 0.9 %, respectively, which were nearly 6 times and 4.4 times higher than those of the pure HPLs.
33357920	4	19	theme	mats	672:675	arg1	composites					693:702	the nanofiber mats reinforced HPLs composites	658:702	the nanofiber mats reinforced HPLs composites	658:702	Specifically, the tensile strength and elongation at break of the nanofiber mats reinforced HPLs composites increased remarkably to 40.8 ±1.1 MPa and 27.9 ± 0.9 %, respectively, which were nearly 6 times and 4.4 times higher than those of the pure HPLs.
33357920	2	20	theme	CA	368:369	arg1	mat					381:383	the CA nanofiber mat	364:383	the CA nanofiber mat	364:383	When the concentration of CA was 16 wt%, SEM images demonstrated that the morphology of the CA nanofiber mat was the best, with an average diameter of 654±246 nm.
33357920	2	21	theme	nanofiber	371:379	arg1	mat					381:383	the CA nanofiber mat	364:383	the CA nanofiber mat	364:383	When the concentration of CA was 16 wt%, SEM images demonstrated that the morphology of the CA nanofiber mat was the best, with an average diameter of 654±246 nm.
33357920	3	22	theme	composites	556:565	arg1	properties					524:533	the mechanical properties	509:533	the mechanical properties of the resulted HPLs composites	509:565	When CA nanofiber mats were incorporated into the core layer of HPLs, the mechanical properties of the resulted HPLs composites were significantly improved.
33357920	1	23	theme	non-woven	99:107	arg1	CA					128:129	CA	128:129	CA	128:129	In the work, the non-woven cellulose acetate (CA) nanofiber mats were prepared via electrospinning, and CA nanofiber were incorporated into the core layer of the high-pressure laminates (HPLs).
33357920	1	23	theme	non-woven	99:107	arg1	acetate					119:125	non-woven cellulose acetate	99:125	the non-woven cellulose acetate (CA) nanofiber mats	95:145	In the work, the non-woven cellulose acetate (CA) nanofiber mats were prepared via electrospinning, and CA nanofiber were incorporated into the core layer of the high-pressure laminates (HPLs).
33357920	1	24	theme	CA	186:187	arg1	nanofiber					189:197	CA nanofiber	186:197	CA nanofiber	186:197	In the work, the non-woven cellulose acetate (CA) nanofiber mats were prepared via electrospinning, and CA nanofiber were incorporated into the core layer of the high-pressure laminates (HPLs).
33357920	0	25	theme	electrospun	40:50	arg1	nanofibers					70:79	electrospun cellulose acetate nanofibers	40:79	electrospun cellulose acetate nanofibers	40:79	High pressure laminates reinforced with electrospun cellulose acetate nanofibers.
33357920	5	26	theme	nanofiber	891:899	arg1	mats					901:904	the CA nanofiber mats	884:904	the CA nanofiber mats	884:904	Furthermore, the incorporation of the CA nanofiber mats also significantly improved the flame retardancy of the HPLs, which was revealed from the thermogravimetric analysis (TGA) results.
33357920	5	27	theme	TGA	1024:1026	arg1	results					1029:1035	the thermogravimetric analysis (TGA) results	992:1035	the thermogravimetric analysis (TGA) results	992:1035	Furthermore, the incorporation of the CA nanofiber mats also significantly improved the flame retardancy of the HPLs, which was revealed from the thermogravimetric analysis (TGA) results.
33357920	3	28	theme	resulted	542:549	arg1	composites					556:565	the resulted HPLs composites	538:565	the resulted HPLs composites	538:565	When CA nanofiber mats were incorporated into the core layer of HPLs, the mechanical properties of the resulted HPLs composites were significantly improved.
33357920	5	29	theme	mats	901:904	arg1	incorporation					867:879	the incorporation	863:879	the incorporation of the CA nanofiber mats	863:904	Furthermore, the incorporation of the CA nanofiber mats also significantly improved the flame retardancy of the HPLs, which was revealed from the thermogravimetric analysis (TGA) results.
33357920	4	30	theme	pure	839:842	arg1	HPLs					844:847	the pure HPLs	835:847	the pure HPLs	835:847	Specifically, the tensile strength and elongation at break of the nanofiber mats reinforced HPLs composites increased remarkably to 40.8 ±1.1 MPa and 27.9 ± 0.9 %, respectively, which were nearly 6 times and 4.4 times higher than those of the pure HPLs.
33357920	0	31	theme	acetate	62:68	arg1	nanofibers					70:79	electrospun cellulose acetate nanofibers	40:79	electrospun cellulose acetate nanofibers	40:79	High pressure laminates reinforced with electrospun cellulose acetate nanofibers.
33357920	3	32	theme	HPLs	551:554	arg1	composites					556:565	the resulted HPLs composites	538:565	the resulted HPLs composites	538:565	When CA nanofiber mats were incorporated into the core layer of HPLs, the mechanical properties of the resulted HPLs composites were significantly improved.
33357920	2	33	theme	16	309:310	arg1	wt					312:313	wt	312:313	wt	312:313	When the concentration of CA was 16 wt%, SEM images demonstrated that the morphology of the CA nanofiber mat was the best, with an average diameter of 654±246 nm.
33357920	0	34	theme	cellulose	52:60	arg1	nanofibers					70:79	electrospun cellulose acetate nanofibers	40:79	electrospun cellulose acetate nanofibers	40:79	High pressure laminates reinforced with electrospun cellulose acetate nanofibers.
33357920	3	35	theme	HPLs	503:506	arg1	layer					494:498	the core layer	485:498	the core layer of HPLs	485:506	When CA nanofiber mats were incorporated into the core layer of HPLs, the mechanical properties of the resulted HPLs composites were significantly improved.
33357920	1	36	theme	acetate	119:125	arg1	mats					142:145	the non-woven cellulose acetate (CA) nanofiber mats	95:145	the non-woven cellulose acetate (CA) nanofiber mats	95:145	In the work, the non-woven cellulose acetate (CA) nanofiber mats were prepared via electrospinning, and CA nanofiber were incorporated into the core layer of the high-pressure laminates (HPLs).
33357920	4	37	from	break	649:653	arg1	elongation					635:644	elongation	635:644	elongation	635:644	Specifically, the tensile strength and elongation at break of the nanofiber mats reinforced HPLs composites increased remarkably to 40.8 ±1.1 MPa and 27.9 ± 0.9 %, respectively, which were nearly 6 times and 4.4 times higher than those of the pure HPLs.
33357920	4	37	from	break	649:653	arg1	strength					622:629	tensile strength	614:629	tensile strength	614:629	Specifically, the tensile strength and elongation at break of the nanofiber mats reinforced HPLs composites increased remarkably to 40.8 ±1.1 MPa and 27.9 ± 0.9 %, respectively, which were nearly 6 times and 4.4 times higher than those of the pure HPLs.
33357920	2	38	theme	CA	302:303	arg1	%					314:314	16 wt%	309:314	16 wt%	309:314	When the concentration of CA was 16 wt%, SEM images demonstrated that the morphology of the CA nanofiber mat was the best, with an average diameter of 654±246 nm.
33357920	2	38	theme	CA	302:303	arg1	concentration					285:297	the concentration	281:297	the concentration of CA	281:303	When the concentration of CA was 16 wt%, SEM images demonstrated that the morphology of the CA nanofiber mat was the best, with an average diameter of 654±246 nm.
33357920	5	39	theme	flame	938:942	arg1	retardancy					944:953	the flame retardancy	934:953	the flame retardancy of the HPLs, which was revealed from the thermogravimetric analysis (TGA) results	934:1035	Furthermore, the incorporation of the CA nanofiber mats also significantly improved the flame retardancy of the HPLs, which was revealed from the thermogravimetric analysis (TGA) results.
33357920	0	40	dep	laminates	14:22	arg1	reinforced					24:33	reinforced	24:33	laminates reinforced with electrospun cellulose acetate nanofibers	14:79	High pressure laminates reinforced with electrospun cellulose acetate nanofibers.
33357920	3	41	theme	CA	444:445	arg1	mats					457:460	CA nanofiber mats	444:460	CA nanofiber mats	444:460	When CA nanofiber mats were incorporated into the core layer of HPLs, the mechanical properties of the resulted HPLs composites were significantly improved.
33357920	4	42	dep	MPa	738:740	arg1	to					725:726	to	725:726	to	725:726	Specifically, the tensile strength and elongation at break of the nanofiber mats reinforced HPLs composites increased remarkably to 40.8 ±1.1 MPa and 27.9 ± 0.9 %, respectively, which were nearly 6 times and 4.4 times higher than those of the pure HPLs.
33357920	3	43	theme	mechanical	513:522	arg1	properties					524:533	the mechanical properties	509:533	the mechanical properties of the resulted HPLs composites	509:565	When CA nanofiber mats were incorporated into the core layer of HPLs, the mechanical properties of the resulted HPLs composites were significantly improved.
33357920	2	44	theme	SEM	317:319	arg1	images					321:326	SEM images	317:326	SEM images	317:326	When the concentration of CA was 16 wt%, SEM images demonstrated that the morphology of the CA nanofiber mat was the best, with an average diameter of 654±246 nm.
33357920	5	45	theme	CA	888:889	arg1	mats					901:904	the CA nanofiber mats	884:904	the CA nanofiber mats	884:904	Furthermore, the incorporation of the CA nanofiber mats also significantly improved the flame retardancy of the HPLs, which was revealed from the thermogravimetric analysis (TGA) results.
33357920	1	46	theme	nanofiber	132:140	arg1	mats					142:145	the non-woven cellulose acetate (CA) nanofiber mats	95:145	the non-woven cellulose acetate (CA) nanofiber mats	95:145	In the work, the non-woven cellulose acetate (CA) nanofiber mats were prepared via electrospinning, and CA nanofiber were incorporated into the core layer of the high-pressure laminates (HPLs).
33357920	4	47	dep	strength	622:629	arg1	the					610:612	the	610:612	the	610:612	Specifically, the tensile strength and elongation at break of the nanofiber mats reinforced HPLs composites increased remarkably to 40.8 ±1.1 MPa and 27.9 ± 0.9 %, respectively, which were nearly 6 times and 4.4 times higher than those of the pure HPLs.
33357920	1	48	theme	core	226:229	arg1	layer					231:235	the core layer	222:235	the core layer of the high-pressure laminates (HPLs)	222:273	In the work, the non-woven cellulose acetate (CA) nanofiber mats were prepared via electrospinning, and CA nanofiber were incorporated into the core layer of the high-pressure laminates (HPLs).
34364630	3	0	theme	high-purity	532:542	arg1	GOS					544:546	a 77.4% high-purity GOS	524:546	a 77.4% high-purity GOS products	524:555	Next, we performed compositional characterization of NeoGOS-P70, which confirmed that it was a 77.4% high-purity GOS products, including a large amount of 4'-galactosyllactose.
34364630	0	1	from	analysis	12:19	arg1	sulfate					62:68	dextran sulfate	54:68	dextran sulfate	54:68	Composition analysis and oral administered effects on dextran sulfate sodium-induced colitis of galactooligosaccharides bioconverted by Bacillus circulans.
34364630	3	2	theme	GOS	544:546	arg1	4'-galactosyllactose					586:605	4'-galactosyllactose	586:605	4'-galactosyllactose	586:605	Next, we performed compositional characterization of NeoGOS-P70, which confirmed that it was a 77.4% high-purity GOS products, including a large amount of 4'-galactosyllactose.
34364630	3	2	theme	GOS	544:546	arg1	amount					576:581	a large amount	568:581	a large amount of 4'-galactosyllactose	568:605	Next, we performed compositional characterization of NeoGOS-P70, which confirmed that it was a 77.4% high-purity GOS products, including a large amount of 4'-galactosyllactose.
34364630	3	2	theme	GOS	544:546	arg1	products					548:555	a 77.4% high-purity GOS products	524:555	a 77.4% high-purity GOS products	524:555	Next, we performed compositional characterization of NeoGOS-P70, which confirmed that it was a 77.4% high-purity GOS products, including a large amount of 4'-galactosyllactose.
34364630	6	3	theme	colitis	1168:1174	arg1	agent					1176:1180	an anti-ulcerative colitis agent	1149:1180	an anti-ulcerative colitis agent that could regulate inflammatory responses	1149:1223	These results suggest the potential of the novel GOS, NeoGOS-P70, as an anti-ulcerative colitis agent that could regulate inflammatory responses.
34364630	6	3	theme	colitis	1168:1174	arg1	potential					1106:1114	the potential	1102:1114	the potential of the novel GOS, NeoGOS-P70	1102:1143	These results suggest the potential of the novel GOS, NeoGOS-P70, as an anti-ulcerative colitis agent that could regulate inflammatory responses.
34364630	4	4	theme	mediators	845:853	arg1	loss					776:779	weight loss	769:779	weight loss	769:779	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	4	4	theme	mediators	845:853	arg1	reduction					782:790	reduction	782:790	reduction in colon shortening	782:810	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	4	4	theme	mediators	845:853	arg1	suppression					817:827	suppression	817:827	suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice	817:972	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	4	5	theme	ulcerative	950:959	arg1	colitis					961:967	ulcerative colitis	950:967	ulcerative colitis mice	950:972	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	5	6	from	tissue	1026:1031	arg1	analysis					988:995	Histological analysis	975:995	Histological analysis of mucin expression in colon tissue	975:1031	Histological analysis of mucin expression in colon tissue revealed the protective effects of NeoGOS-P70.
34364630	3	7	theme	large	570:574	arg1	4'-galactosyllactose					586:605	4'-galactosyllactose	586:605	4'-galactosyllactose	586:605	Next, we performed compositional characterization of NeoGOS-P70, which confirmed that it was a 77.4% high-purity GOS products, including a large amount of 4'-galactosyllactose.
34364630	3	7	theme	large	570:574	arg1	amount					576:581	a large amount	568:581	a large amount of 4'-galactosyllactose	568:605	Next, we performed compositional characterization of NeoGOS-P70, which confirmed that it was a 77.4% high-purity GOS products, including a large amount of 4'-galactosyllactose.
34364630	4	8	theme	colitis	643:649	arg1	model					651:655	DSS-induced colitis model	631:655	DSS-induced colitis model	631:655	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	0	9	theme	galactooligosaccharides	96:118	arg1	colitis					85:91	colitis	85:91	colitis of galactooligosaccharides bioconverted by Bacillus circulans	85:153	Composition analysis and oral administered effects on dextran sulfate sodium-induced colitis of galactooligosaccharides bioconverted by Bacillus circulans.
34364630	1	10	contain	have	199:202	arg1	Galactooligosaccharides					156:178	Galactooligosaccharides	156:178	Galactooligosaccharides	156:178	Galactooligosaccharides have been known to have many health benefits as prebiotic ingredients.
34364630	1	10	contain	have	199:202	arg2	ingredients					238:248	prebiotic ingredients	228:248	prebiotic ingredients	228:248	Galactooligosaccharides have been known to have many health benefits as prebiotic ingredients.
34364630	1	10	contain	have	199:202	arg2	benefits					216:223	many health benefits	204:223	many health benefits	204:223	Galactooligosaccharides have been known to have many health benefits as prebiotic ingredients.
34364630	5	11	theme	protective	1046:1055	arg1	effects					1057:1063	the protective effects	1042:1063	the protective effects of NeoGOS-P70	1042:1077	Histological analysis of mucin expression in colon tissue revealed the protective effects of NeoGOS-P70.
34364630	4	12	from	suppression	817:827	arg1	shortening					801:810	colon shortening	795:810	colon shortening	795:810	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	2	13	theme	galactooligosaccharide	316:337	arg1	activity					300:307	the anti-inflammatory activity	278:307	the anti-inflammatory activity of the galactooligosaccharide, NeoGOS-P70 (Korean commercial product), in a dextran sodium sulfate-induced colitis model	278:428	In this study, we examined the anti-inflammatory activity of the galactooligosaccharide, NeoGOS-P70 (Korean commercial product), in a dextran sodium sulfate-induced colitis model.
34364630	4	14	theme	DSS-induced	631:641	arg1	colitis					643:649	DSS-induced colitis	631:649	DSS-induced colitis model	631:655	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	4	15	theme	colon	795:799	arg1	shortening					801:810	colon shortening	795:810	colon shortening	795:810	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	4	16	theme	inflammatory	832:843	arg1	mediators					845:853	inflammatory mediators	832:853	inflammatory mediators	832:853	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	4	16	theme	inflammatory	832:843	arg1	secretion					926:934	interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion	866:934	interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice	866:972	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	1	17	theme	many	204:207	arg1	benefits					216:223	many health benefits	204:223	many health benefits	204:223	Galactooligosaccharides have been known to have many health benefits as prebiotic ingredients.
34364630	1	17	theme	many	204:207	arg1	ingredients					238:248	prebiotic ingredients	228:248	prebiotic ingredients	228:248	Galactooligosaccharides have been known to have many health benefits as prebiotic ingredients.
34364630	4	18	from	experiments	616:626	arg1	model					651:655	DSS-induced colitis model	631:655	DSS-induced colitis model	631:655	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	0	19	from	effects	43:49	arg1	sulfate					62:68	dextran sulfate	54:68	dextran sulfate	54:68	Composition analysis and oral administered effects on dextran sulfate sodium-induced colitis of galactooligosaccharides bioconverted by Bacillus circulans.
34364630	6	20	theme	anti-ulcerative	1152:1166	arg1	agent					1176:1180	an anti-ulcerative colitis agent	1149:1180	an anti-ulcerative colitis agent that could regulate inflammatory responses	1149:1223	These results suggest the potential of the novel GOS, NeoGOS-P70, as an anti-ulcerative colitis agent that could regulate inflammatory responses.
34364630	6	20	theme	anti-ulcerative	1152:1166	arg1	potential					1106:1114	the potential	1102:1114	the potential of the novel GOS, NeoGOS-P70	1102:1143	These results suggest the potential of the novel GOS, NeoGOS-P70, as an anti-ulcerative colitis agent that could regulate inflammatory responses.
34364630	1	21	theme	health	209:214	arg1	benefits					216:223	many health benefits	204:223	many health benefits	204:223	Galactooligosaccharides have been known to have many health benefits as prebiotic ingredients.
34364630	1	21	theme	health	209:214	arg1	ingredients					238:248	prebiotic ingredients	228:248	prebiotic ingredients	228:248	Galactooligosaccharides have been known to have many health benefits as prebiotic ingredients.
34364630	5	22	theme	Histological	975:986	arg1	analysis					988:995	Histological analysis	975:995	Histological analysis of mucin expression in colon tissue	975:1031	Histological analysis of mucin expression in colon tissue revealed the protective effects of NeoGOS-P70.
34364630	5	23	from	expression	1006:1015	arg1	tissue					1026:1031	colon tissue	1020:1031	colon tissue	1020:1031	Histological analysis of mucin expression in colon tissue revealed the protective effects of NeoGOS-P70.
34364630	2	24	theme	commercial	359:368	arg1	product					370:376	Korean commercial product	352:376	Korean commercial product	352:376	In this study, we examined the anti-inflammatory activity of the galactooligosaccharide, NeoGOS-P70 (Korean commercial product), in a dextran sodium sulfate-induced colitis model.
34364630	2	24	theme	commercial	359:368	arg1	NeoGOS-P70					340:349	NeoGOS-P70	340:349	NeoGOS-P70 (Korean commercial product)	340:377	In this study, we examined the anti-inflammatory activity of the galactooligosaccharide, NeoGOS-P70 (Korean commercial product), in a dextran sodium sulfate-induced colitis model.
34364630	4	25	theme	colitis	743:749	arg1	suppression					817:827	suppression	817:827	suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice	817:972	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	4	25	theme	colitis	743:749	arg1	loss					776:779	weight loss	769:779	weight loss	769:779	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	4	25	theme	colitis	743:749	arg1	symptoms					751:758	DSS-induced colitis symptoms	731:758	DSS-induced colitis symptoms	731:758	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	4	25	theme	colitis	743:749	arg1	reduction					782:790	reduction	782:790	reduction in colon shortening	782:810	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	0	26	theme	Composition	0:10	arg1	analysis					12:19	Composition analysis	0:19	Composition analysis	0:19	Composition analysis and oral administered effects on dextran sulfate sodium-induced colitis of galactooligosaccharides bioconverted by Bacillus circulans.
34364630	4	27	theme	myeloperoxidase	910:924	arg1	secretion					926:934	interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion	866:934	interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice	866:972	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	5	28	from	analysis	988:995	arg1	tissue					1026:1031	colon tissue	1020:1031	colon tissue	1020:1031	Histological analysis of mucin expression in colon tissue revealed the protective effects of NeoGOS-P70.
34364630	2	29	theme	Korean	352:357	arg1	product					370:376	Korean commercial product	352:376	Korean commercial product	352:376	In this study, we examined the anti-inflammatory activity of the galactooligosaccharide, NeoGOS-P70 (Korean commercial product), in a dextran sodium sulfate-induced colitis model.
34364630	2	29	theme	Korean	352:357	arg1	NeoGOS-P70					340:349	NeoGOS-P70	340:349	NeoGOS-P70 (Korean commercial product)	340:377	In this study, we examined the anti-inflammatory activity of the galactooligosaccharide, NeoGOS-P70 (Korean commercial product), in a dextran sodium sulfate-induced colitis model.
34364630	2	30	theme	colitis	416:422	arg1	model					424:428	a dextran sodium sulfate-induced colitis model	383:428	a dextran sodium sulfate-induced colitis model	383:428	In this study, we examined the anti-inflammatory activity of the galactooligosaccharide, NeoGOS-P70 (Korean commercial product), in a dextran sodium sulfate-induced colitis model.
34364630	0	31	theme	oral	25:28	arg1	effects					43:49	oral administered effects	25:49	oral administered effects on dextran sulfate	25:68	Composition analysis and oral administered effects on dextran sulfate sodium-induced colitis of galactooligosaccharides bioconverted by Bacillus circulans.
34364630	1	32	theme	prebiotic	228:236	arg1	benefits					216:223	many health benefits	204:223	many health benefits	204:223	Galactooligosaccharides have been known to have many health benefits as prebiotic ingredients.
34364630	1	32	theme	prebiotic	228:236	arg1	ingredients					238:248	prebiotic ingredients	228:248	prebiotic ingredients	228:248	Galactooligosaccharides have been known to have many health benefits as prebiotic ingredients.
34364630	5	33	theme	colon	1020:1024	arg1	tissue					1026:1031	colon tissue	1020:1031	colon tissue	1020:1031	Histological analysis of mucin expression in colon tissue revealed the protective effects of NeoGOS-P70.
34364630	4	34	theme	Further	608:614	arg1	experiments					616:626	Further experiments	608:626	Further experiments in DSS-induced colitis model	608:655	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	3	35	theme	4'-galactosyllactose	586:605	arg1	4'-galactosyllactose					586:605	4'-galactosyllactose	586:605	4'-galactosyllactose	586:605	Next, we performed compositional characterization of NeoGOS-P70, which confirmed that it was a 77.4% high-purity GOS products, including a large amount of 4'-galactosyllactose.
34364630	3	35	theme	4'-galactosyllactose	586:605	arg1	amount					576:581	a large amount	568:581	a large amount of 4'-galactosyllactose	568:605	Next, we performed compositional characterization of NeoGOS-P70, which confirmed that it was a 77.4% high-purity GOS products, including a large amount of 4'-galactosyllactose.
34364630	2	36	from	activity	300:307	arg1	model					424:428	a dextran sodium sulfate-induced colitis model	383:428	a dextran sodium sulfate-induced colitis model	383:428	In this study, we examined the anti-inflammatory activity of the galactooligosaccharide, NeoGOS-P70 (Korean commercial product), in a dextran sodium sulfate-induced colitis model.
34364630	6	37	theme	GOS	1129:1131	arg1	agent					1176:1180	an anti-ulcerative colitis agent	1149:1180	an anti-ulcerative colitis agent that could regulate inflammatory responses	1149:1223	These results suggest the potential of the novel GOS, NeoGOS-P70, as an anti-ulcerative colitis agent that could regulate inflammatory responses.
34364630	6	37	theme	GOS	1129:1131	arg1	potential					1106:1114	the potential	1102:1114	the potential of the novel GOS, NeoGOS-P70	1102:1143	These results suggest the potential of the novel GOS, NeoGOS-P70, as an anti-ulcerative colitis agent that could regulate inflammatory responses.
34364630	4	38	theme	weight	769:774	arg1	loss					776:779	weight loss	769:779	weight loss	769:779	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	6	39	theme	inflammatory	1202:1213	arg1	responses					1215:1223	inflammatory responses	1202:1223	inflammatory responses	1202:1223	These results suggest the potential of the novel GOS, NeoGOS-P70, as an anti-ulcerative colitis agent that could regulate inflammatory responses.
34364630	4	40	theme	tumor	881:885	arg1	factor-α					896:903	tumor necrosis factor-α	881:903	tumor necrosis factor-α	881:903	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	4	41	theme	DSS-induced	731:741	arg1	suppression					817:827	suppression	817:827	suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice	817:972	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	4	41	theme	DSS-induced	731:741	arg1	loss					776:779	weight loss	769:779	weight loss	769:779	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	4	41	theme	DSS-induced	731:741	arg1	symptoms					751:758	DSS-induced colitis symptoms	731:758	DSS-induced colitis symptoms	731:758	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	4	41	theme	DSS-induced	731:741	arg1	reduction					782:790	reduction	782:790	reduction in colon shortening	782:810	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	6	42	theme	novel	1123:1127	arg1	GOS					1129:1131	the novel GOS	1119:1131	the novel GOS	1119:1131	These results suggest the potential of the novel GOS, NeoGOS-P70, as an anti-ulcerative colitis agent that could regulate inflammatory responses.
34364630	6	42	theme	novel	1123:1127	arg1	NeoGOS-P70					1134:1143	NeoGOS-P70	1134:1143	NeoGOS-P70	1134:1143	These results suggest the potential of the novel GOS, NeoGOS-P70, as an anti-ulcerative colitis agent that could regulate inflammatory responses.
34364630	0	43	theme	administered	30:41	arg1	effects					43:49	oral administered effects	25:49	oral administered effects on dextran sulfate	25:68	Composition analysis and oral administered effects on dextran sulfate sodium-induced colitis of galactooligosaccharides bioconverted by Bacillus circulans.
34364630	2	44	theme	dextran	385:391	arg1	model					424:428	a dextran sodium sulfate-induced colitis model	383:428	a dextran sodium sulfate-induced colitis model	383:428	In this study, we examined the anti-inflammatory activity of the galactooligosaccharide, NeoGOS-P70 (Korean commercial product), in a dextran sodium sulfate-induced colitis model.
34364630	3	45	theme	compositional	450:462	arg1	characterization					464:479	compositional characterization	450:479	compositional characterization	450:479	Next, we performed compositional characterization of NeoGOS-P70, which confirmed that it was a 77.4% high-purity GOS products, including a large amount of 4'-galactosyllactose.
34364630	4	46	theme	NeoGOS-P70	692:701	arg1	administration					674:687	oral administration	669:687	oral administration of NeoGOS-P70	669:701	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	0	47	theme	dextran	54:60	arg1	sulfate					62:68	dextran sulfate	54:68	dextran sulfate	54:68	Composition analysis and oral administered effects on dextran sulfate sodium-induced colitis of galactooligosaccharides bioconverted by Bacillus circulans.
34364630	4	48	theme	mice	969:972	arg1	colon					941:945	colon	941:945	colon of ulcerative colitis mice	941:972	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	3	49	theme	NeoGOS-P70	484:493	arg1	characterization					464:479	compositional characterization	450:479	compositional characterization	450:479	Next, we performed compositional characterization of NeoGOS-P70, which confirmed that it was a 77.4% high-purity GOS products, including a large amount of 4'-galactosyllactose.
34364630	4	50	theme	factor-α	896:903	arg1	secretion					926:934	interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion	866:934	interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice	866:972	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	3	51	theme	77.4	526:529	arg1	%					530:530	%	530:530	%	530:530	Next, we performed compositional characterization of NeoGOS-P70, which confirmed that it was a 77.4% high-purity GOS products, including a large amount of 4'-galactosyllactose.
34364630	4	52	theme	colitis	961:967	arg1	mice					969:972	ulcerative colitis mice	950:972	ulcerative colitis mice	950:972	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	4	53	theme	necrosis	887:894	arg1	factor-α					896:903	tumor necrosis factor-α	881:903	tumor necrosis factor-α	881:903	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	3	54	theme	%	530:530	arg1	GOS					544:546	a 77.4% high-purity GOS	524:546	a 77.4% high-purity GOS products	524:555	Next, we performed compositional characterization of NeoGOS-P70, which confirmed that it was a 77.4% high-purity GOS products, including a large amount of 4'-galactosyllactose.
34364630	4	55	from	reduction	782:790	arg1	shortening					801:810	colon shortening	795:810	colon shortening	795:810	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	5	56	theme	NeoGOS-P70	1068:1077	arg1	effects					1057:1063	the protective effects	1042:1063	the protective effects of NeoGOS-P70	1042:1077	Histological analysis of mucin expression in colon tissue revealed the protective effects of NeoGOS-P70.
34364630	5	57	theme	mucin	1000:1004	arg1	expression					1006:1015	mucin expression	1000:1015	mucin expression in colon tissue	1000:1031	Histological analysis of mucin expression in colon tissue revealed the protective effects of NeoGOS-P70.
34364630	2	58	theme	anti-inflammatory	282:298	arg1	activity					300:307	the anti-inflammatory activity	278:307	the anti-inflammatory activity of the galactooligosaccharide, NeoGOS-P70 (Korean commercial product), in a dextran sodium sulfate-induced colitis model	278:428	In this study, we examined the anti-inflammatory activity of the galactooligosaccharide, NeoGOS-P70 (Korean commercial product), in a dextran sodium sulfate-induced colitis model.
34364630	4	59	from	colon	941:945	arg1	secretion					926:934	interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion	866:934	interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice	866:972	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	5	60	theme	expression	1006:1015	arg1	analysis					988:995	Histological analysis	975:995	Histological analysis of mucin expression in colon tissue	975:1031	Histological analysis of mucin expression in colon tissue revealed the protective effects of NeoGOS-P70.
34364630	4	61	from	loss	776:779	arg1	shortening					801:810	colon shortening	795:810	colon shortening	795:810	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	4	62	theme	interleukin-6	866:878	arg1	secretion					926:934	interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion	866:934	interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice	866:972	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	2	63	theme	sulfate-induced	400:414	arg1	model					424:428	a dextran sodium sulfate-induced colitis model	383:428	a dextran sodium sulfate-induced colitis model	383:428	In this study, we examined the anti-inflammatory activity of the galactooligosaccharide, NeoGOS-P70 (Korean commercial product), in a dextran sodium sulfate-induced colitis model.
34364630	4	64	theme	oral	669:672	arg1	administration					674:687	oral administration	669:687	oral administration of NeoGOS-P70	669:701	Further experiments in DSS-induced colitis model showed that oral administration of NeoGOS-P70 could significantly improve DSS-induced colitis symptoms, such as weight loss, reduction in colon shortening, and suppression of inflammatory mediators, including interleukin-6, tumor necrosis factor-α, and myeloperoxidase secretion from colon of ulcerative colitis mice.
34364630	2	65	theme	sodium	393:398	arg1	model					424:428	a dextran sodium sulfate-induced colitis model	383:428	a dextran sodium sulfate-induced colitis model	383:428	In this study, we examined the anti-inflammatory activity of the galactooligosaccharide, NeoGOS-P70 (Korean commercial product), in a dextran sodium sulfate-induced colitis model.
33483312	6	0	theme	Enterococcus	1923:1934	arg1	faecalis					1936:1943	Enterococcus faecalis	1923:1943	Enterococcus faecalis	1923:1943	Recent studies demonstrated that the cell wall-associated polysaccharide of Enterococcus faecalis is required for susceptibility to bactericidal antibiotic agents.
33483312	4	1	theme	limited	1305:1311	arg1	localization					1313:1324	the limited localization	1301:1324	the limited localization of BacL1	1301:1333	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	4	2	dep	resulted	1161:1168	arg1	caused					1202:1207	caused	1202:1207	caused	1202:1207	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	1	3	theme	Enterococcus	299:310	arg1	cells					321:325	Enterococcus faecalis cells	299:325	Enterococcus faecalis cells that carry the Bac41-coding plasmid	299:361	Enterococcal plasmid-encoded bacteriolysin Bac41 is a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid.
33483312	8	4	from	Firmicutes	2256:2265	arg1	biosynthesis					2177:2188	the biosynthesis	2173:2188	the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen)	2173:2405	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	8	5	theme	faecalis-specific	2311:2327	arg1	antigen					2398:2404	enterococcal polysaccharide antigen	2370:2404	enterococcal polysaccharide antigen	2370:2404	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	8	5	theme	faecalis-specific	2311:2327	arg1	EPA					2365:2367	the E. faecalis-specific cell wall-associated polysaccharide EPA	2304:2367	the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen)	2304:2405	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	4	6	theme	faecalis	1110:1117	arg1	surface					1096:1102	the cell surface	1087:1102	the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents	1087:1528	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	3	7	theme	BacL1	751:755	arg1	effects					740:746	the antimicrobial effects	722:746	the antimicrobial effects of BacL1 and BacA	722:764	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	8	8	theme	wall-associated	2334:2348	arg1	antigen					2398:2404	enterococcal polysaccharide antigen	2370:2404	enterococcal polysaccharide antigen	2370:2404	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	8	8	theme	wall-associated	2334:2348	arg1	EPA					2365:2367	the E. faecalis-specific cell wall-associated polysaccharide EPA	2304:2367	the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen)	2304:2405	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	4	9	theme	cell	1124:1127	arg1	defect					1149:1154	This cell surface composition defect	1119:1154	E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents	1107:1528	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	1	10	theme	selective	211:219	arg1	system					235:240	a selective antimicrobial system	209:240	a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid	209:361	Enterococcal plasmid-encoded bacteriolysin Bac41 is a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid.
33483312	1	10	theme	selective	211:219	arg1	Bac41					200:204	Enterococcal plasmid-encoded bacteriolysin Bac41	157:204	Enterococcal plasmid-encoded bacteriolysin Bac41	157:204	Enterococcal plasmid-encoded bacteriolysin Bac41 is a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid.
33483312	8	11	theme	galU	2126:2129	arg1	essential					2159:2167	essential	2159:2167	essential	2159:2167	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	8	11	theme	galU	2126:2129	arg1	homologue					2131:2139	The galU homologue	2122:2139	The galU homologue	2122:2139	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	4	12	theme	cell	1091:1094	arg1	surface					1096:1102	the cell surface	1087:1102	the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents	1087:1528	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	5	13	theme	cell	1702:1705	arg1	physiology					1707:1716	an intact cell physiology	1692:1716	an intact cell physiology in E. faecalisIMPORTANCE Cell wall-associated polysaccharides of bacteria are involved in various physiological characteristics	1692:1844	These data suggest that a complex mechanism of action underlies lytic killing, as exogenous bacteriolysis induced by lytic bacteriocins or beta-lactams requires an intact cell physiology in E. faecalisIMPORTANCE Cell wall-associated polysaccharides of bacteria are involved in various physiological characteristics.
33483312	0	14	theme	faecalis	123:130	arg1	Susceptibility					92:105	the Susceptibility	88:105	the Susceptibility of Enterococcus faecalis to Bacteriolytic Agents	88:154	Inactivation of GalU Leads to a Cell Wall-Associated Polysaccharide Defect That Reduces the Susceptibility of Enterococcus faecalis to Bacteriolytic Agents.
33483312	7	15	theme	lytic	2097:2101	arg1	Bac41					2115:2119	the enterococcal lytic bacteriocin Bac41	2080:2119	the enterococcal lytic bacteriocin Bac41	2080:2119	Here, we demonstrated that a galU mutation resulted in resistance to the enterococcal lytic bacteriocin Bac41.
33483312	4	16	theme	UDP-glucose	940:950	arg1	depletion					927:935	the depletion	923:935	the depletion of UDP-glucose	923:950	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	8	17	theme	enterococcal	2370:2381	arg1	antigen					2398:2404	enterococcal polysaccharide antigen	2370:2404	enterococcal polysaccharide antigen	2370:2404	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	8	17	theme	enterococcal	2370:2381	arg1	EPA					2365:2367	the E. faecalis-specific cell wall-associated polysaccharide EPA	2304:2367	the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen)	2304:2405	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	6	18	theme	antibiotic	1992:2001	arg1	agents					2003:2008	bactericidal antibiotic agents	1979:2008	bactericidal antibiotic agents	1979:2008	Recent studies demonstrated that the cell wall-associated polysaccharide of Enterococcus faecalis is required for susceptibility to bactericidal antibiotic agents.
33483312	0	19	theme	Bacteriolytic	135:147	arg1	Agents					149:154	Bacteriolytic Agents	135:154	Bacteriolytic Agents	135:154	Inactivation of GalU Leads to a Cell Wall-Associated Polysaccharide Defect That Reduces the Susceptibility of Enterococcus faecalis to Bacteriolytic Agents.
33483312	3	20	theme	truncation	791:800	arg1	deletion					802:809	a truncation deletion	789:809	a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein	789:892	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	3	21	theme	acid	835:838	arg1	region					845:850	the C-terminal amino acid (aa) region 288 to 298	814:861	the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein	814:892	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	3	22	dep	region	845:850	arg1	to					856:857	to	856:857	to	856:857	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	10	23	from	biosynthesis	2567:2578	arg1	faecalis					2586:2593	E. faecalis	2583:2593	E. faecalis	2583:2593	We demonstrated here that galU was essential for EPA biosynthesis in E. faecalis, and EPA production might underlie susceptibility to lytic bacteriocin and antibiotic agents by undefined mechanisms.
33483312	5	24	dep	physiology	1707:1716	arg1	involved					1796:1803	involved	1796:1803	are involved in various physiological characteristics	1792:1844	These data suggest that a complex mechanism of action underlies lytic killing, as exogenous bacteriolysis induced by lytic bacteriocins or beta-lactams requires an intact cell physiology in E. faecalisIMPORTANCE Cell wall-associated polysaccharides of bacteria are involved in various physiological characteristics.
33483312	4	25	theme	composition	1137:1147	arg1	defect					1149:1154	This cell surface composition defect	1119:1154	E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents	1107:1528	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	4	26	theme	bacteriostatic	1470:1483	arg1	agents					1499:1504	other bacteriostatic antimicrobial agents	1464:1504	other bacteriostatic antimicrobial agents	1464:1504	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	0	27	theme	Polysaccharide	53:66	arg1	Defect					68:73	a Cell Wall-Associated Polysaccharide Defect	30:73	a Cell Wall-Associated Polysaccharide Defect That Reduces the Susceptibility of Enterococcus faecalis to Bacteriolytic Agents	30:154	Inactivation of GalU Leads to a Cell Wall-Associated Polysaccharide Defect That Reduces the Susceptibility of Enterococcus faecalis to Bacteriolytic Agents.
33483312	3	28	theme	Bac41	645:649	arg1	system					651:656	the Bac41 system	641:656	the Bac41 system	641:656	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	1	29	theme	Enterococcal	157:168	arg1	system					235:240	a selective antimicrobial system	209:240	a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid	209:361	Enterococcal plasmid-encoded bacteriolysin Bac41 is a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid.
33483312	1	29	theme	Enterococcal	157:168	arg1	Bac41					200:204	Enterococcal plasmid-encoded bacteriolysin Bac41	157:204	Enterococcal plasmid-encoded bacteriolysin Bac41	157:204	Enterococcal plasmid-encoded bacteriolysin Bac41 is a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid.
33483312	3	30	theme	protein	886:892	arg1	region					845:850	the C-terminal amino acid (aa) region 288 to 298	814:861	the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein	814:892	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	3	31	theme	BacA	761:764	arg1	effects					740:746	the antimicrobial effects	722:746	the antimicrobial effects of BacL1 and BacA	722:764	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	3	32	theme	region	845:850	arg1	deletion					802:809	a truncation deletion	789:809	a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein	789:892	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	2	33	dep	E.	474:475	arg1	faecalis					477:484	faecalis	477:484	faecalis	477:484	The Bac41 effector consists of the secreted proteins BacL1 and BacA, which attack the cell wall of the target E. faecalis cell to induce bacteriolysis.
33483312	1	34	theme	bacteriolysin	186:198	arg1	system					235:240	a selective antimicrobial system	209:240	a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid	209:361	Enterococcal plasmid-encoded bacteriolysin Bac41 is a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid.
33483312	1	34	theme	bacteriolysin	186:198	arg1	Bac41					200:204	Enterococcal plasmid-encoded bacteriolysin Bac41	157:204	Enterococcal plasmid-encoded bacteriolysin Bac41	157:204	Enterococcal plasmid-encoded bacteriolysin Bac41 is a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid.
33483312	3	35	theme	translated	870:879	arg1	protein					886:892	the translated GalU protein	866:892	the translated GalU protein	866:892	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	4	36	dep	alterations	1286:1296	arg1	had					1364:1366	had	1364:1366	had	1364:1366	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	4	37	theme	cell	1221:1224	arg1	morphology					1226:1235	an abnormal cell morphology	1209:1235	an abnormal cell morphology	1209:1235	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	3	38	theme	aa	841:842	arg1	region					845:850	the C-terminal amino acid (aa) region 288 to 298	814:861	the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein	814:892	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	10	39	theme	EPA	2563:2565	arg1	biosynthesis					2567:2578	EPA biosynthesis	2563:2578	EPA biosynthesis in E. faecalis	2563:2593	We demonstrated here that galU was essential for EPA biosynthesis in E. faecalis, and EPA production might underlie susceptibility to lytic bacteriocin and antibiotic agents by undefined mechanisms.
33483312	9	40	theme	antibacterial	2460:2472	arg1	agents					2474:2479	antibacterial agents	2460:2479	antibacterial agents	2460:2479	The mutant also displayed reduced susceptibility to antibacterial agents and an abnormal cell morphology.
33483312	2	41	dep	proteins	408:415	arg1	BacA					427:430	BacA	427:430	BacA	427:430	The Bac41 effector consists of the secreted proteins BacL1 and BacA, which attack the cell wall of the target E. faecalis cell to induce bacteriolysis.
33483312	2	41	dep	proteins	408:415	arg1	BacL1					417:421	BacL1	417:421	BacL1	417:421	The Bac41 effector consists of the secreted proteins BacL1 and BacA, which attack the cell wall of the target E. faecalis cell to induce bacteriolysis.
33483312	2	41	dep	proteins	408:415	arg1	proteins					408:415	the secreted proteins BacL1 and BacA	395:430	the secreted proteins BacL1 and BacA	395:430	The Bac41 effector consists of the secreted proteins BacL1 and BacA, which attack the cell wall of the target E. faecalis cell to induce bacteriolysis.
33483312	5	42	theme	Cell	1743:1746	arg1	polysaccharides					1764:1778	faecalisIMPORTANCE Cell wall-associated polysaccharides	1724:1778	faecalisIMPORTANCE Cell wall-associated polysaccharides of bacteria	1724:1790	These data suggest that a complex mechanism of action underlies lytic killing, as exogenous bacteriolysis induced by lytic bacteriocins or beta-lactams requires an intact cell physiology in E. faecalisIMPORTANCE Cell wall-associated polysaccharides of bacteria are involved in various physiological characteristics.
33483312	2	43	theme	target	467:472	arg1	cell					486:489	the target E. faecalis cell	463:489	the target E. faecalis cell	463:489	The Bac41 effector consists of the secreted proteins BacL1 and BacA, which attack the cell wall of the target E. faecalis cell to induce bacteriolysis.
33483312	9	44	theme	reduced	2434:2440	arg1	susceptibility					2442:2455	reduced susceptibility	2434:2455	reduced susceptibility to antibacterial agents	2434:2479	The mutant also displayed reduced susceptibility to antibacterial agents and an abnormal cell morphology.
33483312	1	45	theme	faecalis	312:319	arg1	cells					321:325	Enterococcus faecalis cells	299:325	Enterococcus faecalis cells that carry the Bac41-coding plasmid	299:361	Enterococcal plasmid-encoded bacteriolysin Bac41 is a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid.
33483312	8	46	theme	species-specific	2193:2208	arg1	polysaccharides					2231:2245	species-specific cell wall-associated polysaccharides	2193:2245	species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen)	2193:2405	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	6	47	theme	wall-associated	1889:1903	arg1	polysaccharide					1905:1918	the cell wall-associated polysaccharide	1880:1918	the cell wall-associated polysaccharide of Enterococcus faecalis	1880:1943	Recent studies demonstrated that the cell wall-associated polysaccharide of Enterococcus faecalis is required for susceptibility to bactericidal antibiotic agents.
33483312	2	48	theme	cell	450:453	arg1	wall					455:458	the cell wall	446:458	the cell wall of the target E. faecalis cell to induce bacteriolysis	446:513	The Bac41 effector consists of the secreted proteins BacL1 and BacA, which attack the cell wall of the target E. faecalis cell to induce bacteriolysis.
33483312	0	49	theme	GalU	16:19	arg1	Inactivation					0:11	Inactivation	0:11	Inactivation of GalU	0:19	Inactivation of GalU Leads to a Cell Wall-Associated Polysaccharide Defect That Reduces the Susceptibility of Enterococcus faecalis to Bacteriolytic Agents.
33483312	8	50	theme	wall-associated	2215:2229	arg1	polysaccharides					2231:2245	species-specific cell wall-associated polysaccharides	2193:2245	species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen)	2193:2405	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	5	51	theme	action	1578:1583	arg1	mechanism					1565:1573	a complex mechanism	1555:1573	a complex mechanism of action	1555:1583	These data suggest that a complex mechanism of action underlies lytic killing, as exogenous bacteriolysis induced by lytic bacteriocins or beta-lactams requires an intact cell physiology in E. faecalisIMPORTANCE Cell wall-associated polysaccharides of bacteria are involved in various physiological characteristics.
33483312	4	52	theme	chemical	1510:1517	arg1	detergents					1519:1528	chemical detergents	1510:1528	chemical detergents	1510:1528	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	3	53	theme	C-terminal	818:827	arg1	region					845:850	the C-terminal amino acid (aa) region 288 to 298	814:861	the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein	814:892	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	4	54	theme	enterococcal	1011:1022	arg1	antigen					1039:1045	the enterococcal polysaccharide antigen	1007:1045	the enterococcal polysaccharide antigen (EPA)	1007:1051	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	4	54	theme	enterococcal	1011:1022	arg1	EPA					1048:1050	EPA	1048:1050	EPA	1048:1050	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	5	55	theme	various	1808:1814	arg1	characteristics					1830:1844	various physiological characteristics	1808:1844	various physiological characteristics	1808:1844	These data suggest that a complex mechanism of action underlies lytic killing, as exogenous bacteriolysis induced by lytic bacteriocins or beta-lactams requires an intact cell physiology in E. faecalisIMPORTANCE Cell wall-associated polysaccharides of bacteria are involved in various physiological characteristics.
33483312	0	56	theme	Cell	32:35	arg1	Defect					68:73	a Cell Wall-Associated Polysaccharide Defect	30:73	a Cell Wall-Associated Polysaccharide Defect That Reduces the Susceptibility of Enterococcus faecalis to Bacteriolytic Agents	30:154	Inactivation of GalU Leads to a Cell Wall-Associated Polysaccharide Defect That Reduces the Susceptibility of Enterococcus faecalis to Bacteriolytic Agents.
33483312	6	57	theme	Recent	1847:1852	arg1	studies					1854:1860	Recent studies	1847:1860	Recent studies	1847:1860	Recent studies demonstrated that the cell wall-associated polysaccharide of Enterococcus faecalis is required for susceptibility to bactericidal antibiotic agents.
33483312	4	58	theme	impaired	1243:1250	arg1	polarity					1252:1259	impaired polarity	1243:1259	impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents	1243:1528	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	2	59	theme	Bac41	368:372	arg1	effector					374:381	The Bac41 effector	364:381	The Bac41 effector	364:381	The Bac41 effector consists of the secreted proteins BacL1 and BacA, which attack the cell wall of the target E. faecalis cell to induce bacteriolysis.
33483312	3	60	dep	E.	661:662	arg1	faecalis					664:671	faecalis	664:671	faecalis	664:671	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	5	61	theme	exogenous	1613:1621	arg1	bacteriolysis					1623:1635	exogenous bacteriolysis	1613:1635	exogenous bacteriolysis induced by lytic bacteriocins or beta-lactams	1613:1681	These data suggest that a complex mechanism of action underlies lytic killing, as exogenous bacteriolysis induced by lytic bacteriocins or beta-lactams requires an intact cell physiology in E. faecalisIMPORTANCE Cell wall-associated polysaccharides of bacteria are involved in various physiological characteristics.
33483312	5	62	theme	bacteria	1783:1790	arg1	polysaccharides					1764:1778	faecalisIMPORTANCE Cell wall-associated polysaccharides	1724:1778	faecalisIMPORTANCE Cell wall-associated polysaccharides of bacteria	1724:1790	These data suggest that a complex mechanism of action underlies lytic killing, as exogenous bacteriolysis induced by lytic bacteriocins or beta-lactams requires an intact cell physiology in E. faecalisIMPORTANCE Cell wall-associated polysaccharides of bacteria are involved in various physiological characteristics.
33483312	3	63	theme	amino	829:833	arg1	region					845:850	the C-terminal amino acid (aa) region 288 to 298	814:861	the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein	814:892	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	8	64	from	polysaccharides	2231:2245	arg1	Firmicutes					2256:2265	other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen)	2250:2405	other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen)	2250:2405	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	4	65	theme	BacL1	1329:1333	arg1	localization					1313:1324	the limited localization	1301:1324	the limited localization of BacL1	1301:1333	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	1	66	theme	competitive	274:284	arg1	advantage					286:294	a competitive advantage	272:294	a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid	272:361	Enterococcal plasmid-encoded bacteriolysin Bac41 is a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid.
33483312	8	67	theme	galU	2287:2290	arg1	mutant					2292:2297	the galU mutant	2283:2297	the galU mutant	2283:2297	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	6	68	theme	faecalis	1936:1943	arg1	polysaccharide					1905:1918	the cell wall-associated polysaccharide	1880:1918	the cell wall-associated polysaccharide of Enterococcus faecalis	1880:1943	Recent studies demonstrated that the cell wall-associated polysaccharide of Enterococcus faecalis is required for susceptibility to bactericidal antibiotic agents.
33483312	5	69	theme	lytic	1595:1599	arg1	killing					1601:1607	lytic killing	1595:1607	lytic killing	1595:1607	These data suggest that a complex mechanism of action underlies lytic killing, as exogenous bacteriolysis induced by lytic bacteriocins or beta-lactams requires an intact cell physiology in E. faecalisIMPORTANCE Cell wall-associated polysaccharides of bacteria are involved in various physiological characteristics.
33483312	3	70	theme	antimicrobial	726:738	arg1	effects					740:746	the antimicrobial effects	722:746	the antimicrobial effects of BacL1 and BacA	722:764	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	1	71	contain	carry	332:336	arg2	plasmid					355:361	the Bac41-coding plasmid	338:361	the Bac41-coding plasmid	338:361	Enterococcal plasmid-encoded bacteriolysin Bac41 is a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid.
33483312	1	71	contain	carry	332:336	arg1	cells					321:325	Enterococcus faecalis cells	299:325	Enterococcus faecalis cells that carry the Bac41-coding plasmid	299:361	Enterococcal plasmid-encoded bacteriolysin Bac41 is a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid.
33483312	10	72	theme	EPA	2600:2602	arg1	production					2604:2613	EPA production	2600:2613	EPA production	2600:2613	We demonstrated here that galU was essential for EPA biosynthesis in E. faecalis, and EPA production might underlie susceptibility to lytic bacteriocin and antibiotic agents by undefined mechanisms.
33483312	8	73	theme	E.	2308:2309	arg1	antigen					2398:2404	enterococcal polysaccharide antigen	2370:2404	enterococcal polysaccharide antigen	2370:2404	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	8	73	theme	E.	2308:2309	arg1	EPA					2365:2367	the E. faecalis-specific cell wall-associated polysaccharide EPA	2304:2367	the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen)	2304:2405	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	4	74	theme	surface	1129:1135	arg1	defect					1149:1154	This cell surface composition defect	1119:1154	E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents	1107:1528	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	10	75	theme	lytic	2648:2652	arg1	bacteriocin					2654:2664	lytic bacteriocin and antibiotic agents	2648:2686	bacteriocin	2654:2664	We demonstrated here that galU was essential for EPA biosynthesis in E. faecalis, and EPA production might underlie susceptibility to lytic bacteriocin and antibiotic agents by undefined mechanisms.
33483312	4	76	theme	localization	1313:1324	arg1	alterations					1286:1296	alterations	1286:1296	alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents	1286:1528	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	4	76	theme	localization	1313:1324	arg1	division					1273:1280	cell division	1268:1280	cell division	1268:1280	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	8	77	theme	cell	2329:2332	arg1	antigen					2398:2404	enterococcal polysaccharide antigen	2370:2404	enterococcal polysaccharide antigen	2370:2404	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	8	77	theme	cell	2329:2332	arg1	EPA					2365:2367	the E. faecalis-specific cell wall-associated polysaccharide EPA	2304:2367	the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen)	2304:2405	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	5	78	theme	intact	1695:1700	arg1	physiology					1707:1716	an intact cell physiology	1692:1716	an intact cell physiology in E. faecalisIMPORTANCE Cell wall-associated polysaccharides of bacteria are involved in various physiological characteristics	1692:1844	These data suggest that a complex mechanism of action underlies lytic killing, as exogenous bacteriolysis induced by lytic bacteriocins or beta-lactams requires an intact cell physiology in E. faecalisIMPORTANCE Cell wall-associated polysaccharides of bacteria are involved in various physiological characteristics.
33483312	3	79	theme	UTP-glucose-1-phosphate	563:585	arg1	uridylyltransferase					587:605	UTP-glucose-1-phosphate uridylyltransferase	563:605	UTP-glucose-1-phosphate uridylyltransferase	563:605	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	3	79	theme	UTP-glucose-1-phosphate	563:585	arg1	galU					543:546	galU	543:546	galU	543:546	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	1	80	theme	antimicrobial	221:233	arg1	system					235:240	a selective antimicrobial system	209:240	a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid	209:361	Enterococcal plasmid-encoded bacteriolysin Bac41 is a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid.
33483312	1	80	theme	antimicrobial	221:233	arg1	Bac41					200:204	Enterococcal plasmid-encoded bacteriolysin Bac41	157:204	Enterococcal plasmid-encoded bacteriolysin Bac41	157:204	Enterococcal plasmid-encoded bacteriolysin Bac41 is a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid.
33483312	8	81	theme	polysaccharide	2350:2363	arg1	antigen					2398:2404	enterococcal polysaccharide antigen	2370:2404	enterococcal polysaccharide antigen	2370:2404	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	8	81	theme	polysaccharide	2350:2363	arg1	EPA					2365:2367	the E. faecalis-specific cell wall-associated polysaccharide EPA	2304:2367	the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen)	2304:2405	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	5	82	theme	lytic	1648:1652	arg1	bacteriocins					1654:1665	lytic bacteriocins	1648:1665	lytic bacteriocins	1648:1665	These data suggest that a complex mechanism of action underlies lytic killing, as exogenous bacteriolysis induced by lytic bacteriocins or beta-lactams requires an intact cell physiology in E. faecalisIMPORTANCE Cell wall-associated polysaccharides of bacteria are involved in various physiological characteristics.
33483312	9	83	theme	abnormal	2488:2495	arg1	morphology					2502:2511	an abnormal cell morphology	2485:2511	an abnormal cell morphology	2485:2511	The mutant also displayed reduced susceptibility to antibacterial agents and an abnormal cell morphology.
33483312	7	84	theme	bacteriocin	2103:2113	arg1	Bac41					2115:2119	the enterococcal lytic bacteriocin Bac41	2080:2119	the enterococcal lytic bacteriocin Bac41	2080:2119	Here, we demonstrated that a galU mutation resulted in resistance to the enterococcal lytic bacteriocin Bac41.
33483312	6	85	theme	bactericidal	1979:1990	arg1	agents					2003:2008	bactericidal antibiotic agents	1979:2008	bactericidal antibiotic agents	1979:2008	Recent studies demonstrated that the cell wall-associated polysaccharide of Enterococcus faecalis is required for susceptibility to bactericidal antibiotic agents.
33483312	7	86	theme	enterococcal	2084:2095	arg1	Bac41					2115:2119	the enterococcal lytic bacteriocin Bac41	2080:2119	the enterococcal lytic bacteriocin Bac41	2080:2119	Here, we demonstrated that a galU mutation resulted in resistance to the enterococcal lytic bacteriocin Bac41.
33483312	8	87	theme	polysaccharide	2383:2396	arg1	antigen					2398:2404	enterococcal polysaccharide antigen	2370:2404	enterococcal polysaccharide antigen	2370:2404	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	8	87	theme	polysaccharide	2383:2396	arg1	EPA					2365:2367	the E. faecalis-specific cell wall-associated polysaccharide EPA	2304:2367	the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen)	2304:2405	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	4	88	theme	EPA-specific	1183:1194	arg1	genes					1196:1200	EPA-specific genes	1183:1200	EPA-specific genes	1183:1200	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	4	89	theme	reduced	1368:1374	arg1	susceptibility					1376:1389	reduced susceptibility	1368:1389	reduced susceptibility	1368:1389	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	10	90	theme	undefined	2691:2699	arg1	mechanisms					2701:2710	undefined mechanisms	2691:2710	undefined mechanisms	2691:2710	We demonstrated here that galU was essential for EPA biosynthesis in E. faecalis, and EPA production might underlie susceptibility to lytic bacteriocin and antibiotic agents by undefined mechanisms.
33483312	9	91	theme	cell	2497:2500	arg1	morphology					2502:2511	an abnormal cell morphology	2485:2511	an abnormal cell morphology	2485:2511	The mutant also displayed reduced susceptibility to antibacterial agents and an abnormal cell morphology.
33483312	4	92	theme	increased	1436:1444	arg1	susceptibility					1446:1459	their increased susceptibility	1430:1459	their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents	1430:1528	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	4	93	theme	other	1464:1468	arg1	agents					1499:1504	other bacteriostatic antimicrobial agents	1464:1504	other bacteriostatic antimicrobial agents	1464:1504	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	4	94	dep	faecalis	1110:1117	arg1	defect					1149:1154	This cell surface composition defect	1119:1154	E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents	1107:1528	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	1	95	theme	plasmid-encoded	170:184	arg1	system					235:240	a selective antimicrobial system	209:240	a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid	209:361	Enterococcal plasmid-encoded bacteriolysin Bac41 is a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid.
33483312	1	95	theme	plasmid-encoded	170:184	arg1	Bac41					200:204	Enterococcal plasmid-encoded bacteriolysin Bac41	157:204	Enterococcal plasmid-encoded bacteriolysin Bac41	157:204	Enterococcal plasmid-encoded bacteriolysin Bac41 is a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid.
33483312	3	96	theme	GalU	881:884	arg1	protein					886:892	the translated GalU protein	866:892	the translated GalU protein	866:892	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	0	97	theme	Enterococcus	110:121	arg1	faecalis					123:130	Enterococcus faecalis	110:130	Enterococcus faecalis	110:130	Inactivation of GalU Leads to a Cell Wall-Associated Polysaccharide Defect That Reduces the Susceptibility of Enterococcus faecalis to Bacteriolytic Agents.
33483312	7	98	theme	galU	2040:2043	arg1	mutation					2045:2052	a galU mutation	2038:2052	a galU mutation	2038:2052	Here, we demonstrated that a galU mutation resulted in resistance to the enterococcal lytic bacteriocin Bac41.
33483312	8	99	dep	Firmicutes	2256:2265	arg1	lost					2299:2302	lost	2299:2302	lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen)	2299:2405	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	4	100	contain	had	1364:1366	arg1	mutants					1356:1362	these mutants	1350:1362	these mutants	1350:1362	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	4	100	contain	had	1364:1366	arg2	susceptibility					1376:1389	reduced susceptibility	1368:1389	reduced susceptibility	1368:1389	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	5	101	theme	wall-associated	1748:1762	arg1	polysaccharides					1764:1778	faecalisIMPORTANCE Cell wall-associated polysaccharides	1724:1778	faecalisIMPORTANCE Cell wall-associated polysaccharides of bacteria	1724:1790	These data suggest that a complex mechanism of action underlies lytic killing, as exogenous bacteriolysis induced by lytic bacteriocins or beta-lactams requires an intact cell physiology in E. faecalisIMPORTANCE Cell wall-associated polysaccharides of bacteria are involved in various physiological characteristics.
33483312	8	102	theme	cell	2210:2213	arg1	polysaccharides					2231:2245	species-specific cell wall-associated polysaccharides	2193:2245	species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen)	2193:2405	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	4	103	theme	polysaccharide	1024:1037	arg1	antigen					1039:1045	the enterococcal polysaccharide antigen	1007:1045	the enterococcal polysaccharide antigen (EPA)	1007:1051	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	4	103	theme	polysaccharide	1024:1037	arg1	EPA					1048:1050	EPA	1048:1050	EPA	1048:1050	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	4	104	theme	abnormal	1212:1219	arg1	morphology					1226:1235	an abnormal cell morphology	1209:1235	an abnormal cell morphology	1209:1235	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	8	105	theme	polysaccharides	2231:2245	arg1	biosynthesis					2177:2188	the biosynthesis	2173:2188	the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen)	2173:2405	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	6	106	theme	cell	1884:1887	arg1	polysaccharide					1905:1918	the cell wall-associated polysaccharide	1880:1918	the cell wall-associated polysaccharide of Enterococcus faecalis	1880:1943	Recent studies demonstrated that the cell wall-associated polysaccharide of Enterococcus faecalis is required for susceptibility to bactericidal antibiotic agents.
33483312	3	107	theme	Spontaneous	673:683	arg1	mutants					685:691	E. faecalis Spontaneous mutants	661:691	E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein	661:892	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	3	108	contain	carry	783:787	arg1	effects					740:746	the antimicrobial effects	722:746	the antimicrobial effects of BacL1 and BacA	722:764	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	3	108	contain	carry	783:787	arg2	deletion					802:809	a truncation deletion	789:809	a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein	789:892	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	3	109	from	susceptibility	623:636	arg1	mutants					685:691	E. faecalis Spontaneous mutants	661:691	E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein	661:892	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	4	110	theme	antimicrobial	1485:1497	arg1	agents					1499:1504	other bacteriostatic antimicrobial agents	1464:1504	other bacteriostatic antimicrobial agents	1464:1504	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	10	111	theme	antibiotic	2670:2679	arg1	agents					2681:2686	lytic bacteriocin and antibiotic agents	2648:2686	agents	2681:2686	We demonstrated here that galU was essential for EPA biosynthesis in E. faecalis, and EPA production might underlie susceptibility to lytic bacteriocin and antibiotic agents by undefined mechanisms.
33483312	8	112	theme	other	2250:2254	arg1	Firmicutes					2256:2265	other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen)	2250:2405	other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen)	2250:2405	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	2	113	theme	cell	486:489	arg1	wall					455:458	the cell wall	446:458	the cell wall of the target E. faecalis cell to induce bacteriolysis	446:513	The Bac41 effector consists of the secreted proteins BacL1 and BacA, which attack the cell wall of the target E. faecalis cell to induce bacteriolysis.
33483312	3	114	theme	E.	661:662	arg1	mutants					685:691	E. faecalis Spontaneous mutants	661:691	E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein	661:892	Here, we demonstrated that galU, which encodes UTP-glucose-1-phosphate uridylyltransferase, is involved in susceptibility to the Bac41 system in E. faecalis Spontaneous mutants that developed resistance to the antimicrobial effects of BacL1 and BacA were revealed to carry a truncation deletion of the C-terminal amino acid (aa) region 288 to 298 of the translated GalU protein.
33483312	5	115	theme	faecalisIMPORTANCE	1724:1741	arg1	polysaccharides					1764:1778	faecalisIMPORTANCE Cell wall-associated polysaccharides	1724:1778	faecalisIMPORTANCE Cell wall-associated polysaccharides of bacteria	1724:1790	These data suggest that a complex mechanism of action underlies lytic killing, as exogenous bacteriolysis induced by lytic bacteriocins or beta-lactams requires an intact cell physiology in E. faecalisIMPORTANCE Cell wall-associated polysaccharides of bacteria are involved in various physiological characteristics.
33483312	0	116	theme	Wall-Associated	37:51	arg1	Defect					68:73	a Cell Wall-Associated Polysaccharide Defect	30:73	a Cell Wall-Associated Polysaccharide Defect That Reduces the Susceptibility of Enterococcus faecalis to Bacteriolytic Agents	30:154	Inactivation of GalU Leads to a Cell Wall-Associated Polysaccharide Defect That Reduces the Susceptibility of Enterococcus faecalis to Bacteriolytic Agents.
33483312	1	117	theme	Bac41-coding	342:353	arg1	plasmid					355:361	the Bac41-coding plasmid	338:361	the Bac41-coding plasmid	338:361	Enterococcal plasmid-encoded bacteriolysin Bac41 is a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid.
33483312	2	118	theme	E.	474:475	arg1	cell					486:489	the target E. faecalis cell	463:489	the target E. faecalis cell	463:489	The Bac41 effector consists of the secreted proteins BacL1 and BacA, which attack the cell wall of the target E. faecalis cell to induce bacteriolysis.
33483312	5	119	theme	physiological	1816:1828	arg1	characteristics					1830:1844	various physiological characteristics	1808:1844	various physiological characteristics	1808:1844	These data suggest that a complex mechanism of action underlies lytic killing, as exogenous bacteriolysis induced by lytic bacteriocins or beta-lactams requires an intact cell physiology in E. faecalisIMPORTANCE Cell wall-associated polysaccharides of bacteria are involved in various physiological characteristics.
33483312	8	120	from	biosynthesis	2177:2188	arg1	Firmicutes					2256:2265	other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen)	2250:2405	other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen)	2250:2405	The galU homologue is reported to be essential for the biosynthesis of species-specific cell wall-associated polysaccharides in other Firmicutes In E. faecalis, the galU mutant lost the E. faecalis-specific cell wall-associated polysaccharide EPA (enterococcal polysaccharide antigen).
33483312	5	121	theme	complex	1557:1563	arg1	mechanism					1565:1573	a complex mechanism	1555:1573	a complex mechanism of action	1555:1583	These data suggest that a complex mechanism of action underlies lytic killing, as exogenous bacteriolysis induced by lytic bacteriocins or beta-lactams requires an intact cell physiology in E. faecalisIMPORTANCE Cell wall-associated polysaccharides of bacteria are involved in various physiological characteristics.
33483312	4	122	theme	cell	1268:1271	arg1	division					1273:1280	cell division	1268:1280	cell division	1268:1280	This truncation resulted in the depletion of UDP-glucose, leading to a failure to utilize galactose and produce the enterococcal polysaccharide antigen (EPA), which is expressed abundantly on the cell surface of E. faecalis This cell surface composition defect that resulted from galU or EPA-specific genes caused an abnormal cell morphology, with impaired polarity during cell division and alterations of the limited localization of BacL1 Interestingly, these mutants had reduced susceptibility to beta-lactams besides Bac41, despite their increased susceptibility to other bacteriostatic antimicrobial agents and chemical detergents.
33483312	2	123	theme	secreted	399:406	arg1	BacA					427:430	BacA	427:430	BacA	427:430	The Bac41 effector consists of the secreted proteins BacL1 and BacA, which attack the cell wall of the target E. faecalis cell to induce bacteriolysis.
33483312	2	123	theme	secreted	399:406	arg1	BacL1					417:421	BacL1	417:421	BacL1	417:421	The Bac41 effector consists of the secreted proteins BacL1 and BacA, which attack the cell wall of the target E. faecalis cell to induce bacteriolysis.
33483312	2	123	theme	secreted	399:406	arg1	proteins					408:415	the secreted proteins BacL1 and BacA	395:430	the secreted proteins BacL1 and BacA	395:430	The Bac41 effector consists of the secreted proteins BacL1 and BacA, which attack the cell wall of the target E. faecalis cell to induce bacteriolysis.
34812488	8	0	theme	highest	1646:1652	arg1	growth					1660:1665	the second highest hBMSC growth	1635:1665	the second highest hBMSC growth	1635:1665	The 20% TCP blend had the second highest hBMSC growth, exhibited moderate swelling, and the fastest degradation rate.
34812488	8	1	theme	fastest	1705:1711	arg1	rate					1725:1728	the fastest degradation rate	1701:1728	the fastest degradation rate	1701:1728	The 20% TCP blend had the second highest hBMSC growth, exhibited moderate swelling, and the fastest degradation rate.
34812488	1	2	theme	relevant	290:297	arg1	devices					307:313	clinically relevant medical devices	279:313	clinically relevant medical devices	279:313	Melt extrusion 3D printing has become an attractive additive manufacturing technology to construct degradable scaffolds as tissue precursors in order to create clinically relevant medical devices.
34812488	7	3	theme	most	1519:1522	arg1	growth					1530:1535	the most hBMSC growth	1515:1535	the most hBMSC growth	1515:1535	These in vitro cell viability studies revealed that the highest chitosan concentration blend of 20% favoured the most hBMSC growth, exhibited the most swelling, and showed minimal degradation after 28 days.
34812488	9	4	from	β-TCP	1915:1919	arg1	proliferation					1966:1978	hBMSC 3D proliferation	1957:1978	hBMSC 3D proliferation	1957:1978	Overall, this study demonstrates the first direct comparison of a natural biopolymer-based, that is, chitosan, 3D melt extruded PCL composite with that of a bioceramic-based, that is, β-TCP, PCL composite and their effects on hBMSC 3D proliferation.
34812488	6	5	theme	BMSC	1233:1236	arg1	proliferation					1238:1250	Human BMSC proliferation	1227:1250	Human BMSC proliferation	1227:1250	Human BMSC proliferation was assessed using fluorescence imaging, and quantitated via the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) colorimetric assay.
34812488	10	6	theme	print	2072:2076	arg1	scaffolds					2078:2086	print scaffolds	2072:2086	print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity;	2072:2164	3D melt extruded PCL-based composite scaffolds methodology offers a straightforward way to print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity; and demonstrates their potential use for regenerative, bone repair applications.
34812488	3	7	theme	bone-marrow	744:754	arg1	growth					794:799	human bone-marrow derived mesenchymal stem cell (hBMSC) growth	738:799	human bone-marrow derived mesenchymal stem cell (hBMSC) growth	738:799	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	3	8	theme	chitosan	582:589	arg1	ratios					548:553	Varying ratios	540:553	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP)	540:638	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	3	9	dep	three-dimensional	698:714	arg1	3D					717:718	3D	717:718	3D	717:718	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	4	10	theme	basic	861:865	arg1	requirements					876:887	basic printing requirements	861:887	basic printing requirements as well as biomaterial dynamic mechanical (DMA), elemental, and thermogravimetric (TGA) analysis results	861:992	In this study, basic printing requirements as well as biomaterial dynamic mechanical (DMA), elemental, and thermogravimetric (TGA) analysis results demonstrate material homogeneity as well as thermal stability.
34812488	7	11	theme	highest	1462:1468	arg1	blend					1493:1497	the highest chitosan concentration blend	1458:1497	the highest chitosan concentration blend of 20%	1458:1504	These in vitro cell viability studies revealed that the highest chitosan concentration blend of 20% favoured the most hBMSC growth, exhibited the most swelling, and showed minimal degradation after 28 days.
34812488	3	12	theme	biopolymer	570:579	arg1	ratios					548:553	Varying ratios	540:553	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP)	540:638	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	3	13	link	derived	756:762	arg1	cell					781:784	derived mesenchymal stem cell	756:784	human bone-marrow derived mesenchymal stem cell (hBMSC) growth	738:799	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	3	13	link	derived	756:762	arg1	hBMSC					787:791	hBMSC	787:791	hBMSC	787:791	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	3	14	theme	cell	781:784	arg1	growth					794:799	human bone-marrow derived mesenchymal stem cell (hBMSC) growth	738:799	human bone-marrow derived mesenchymal stem cell (hBMSC) growth	738:799	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	3	15	theme	bioceramic	599:608	arg1	phosphate					624:632	the bioceramic, β-tricalcium phosphate	595:632	the bioceramic, β-tricalcium phosphate (TCP)	595:638	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	3	15	theme	bioceramic	599:608	arg1	TCP					635:637	TCP	635:637	TCP	635:637	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	1	16	theme	degradable	218:227	arg1	scaffolds					229:237	degradable scaffolds	218:237	degradable scaffolds	218:237	Melt extrusion 3D printing has become an attractive additive manufacturing technology to construct degradable scaffolds as tissue precursors in order to create clinically relevant medical devices.
34812488	1	16	theme	degradable	218:227	arg1	precursors					249:258	tissue precursors	242:258	tissue precursors	242:258	Melt extrusion 3D printing has become an attractive additive manufacturing technology to construct degradable scaffolds as tissue precursors in order to create clinically relevant medical devices.
34812488	10	17	theme	enhanced	2144:2151	arg1	bioactivity					2153:2163	enhanced bioactivity	2144:2163	enhanced bioactivity;	2144:2164	3D melt extruded PCL-based composite scaffolds methodology offers a straightforward way to print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity; and demonstrates their potential use for regenerative, bone repair applications.
34812488	3	18	theme	mesenchymal	764:774	arg1	cell					781:784	derived mesenchymal stem cell	756:784	human bone-marrow derived mesenchymal stem cell (hBMSC) growth	738:799	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	3	18	theme	mesenchymal	764:774	arg1	hBMSC					787:791	hBMSC	787:791	hBMSC	787:791	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	6	19	theme	MTT	1381:1383	arg1	assay					1399:1403	the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) colorimetric assay	1313:1403	assay	1399:1403	Human BMSC proliferation was assessed using fluorescence imaging, and quantitated via the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) colorimetric assay.
34812488	7	20	theme	cell	1421:1424	arg1	studies					1436:1442	These in vitro cell viability studies	1406:1442	These in vitro cell viability studies	1406:1442	These in vitro cell viability studies revealed that the highest chitosan concentration blend of 20% favoured the most hBMSC growth, exhibited the most swelling, and showed minimal degradation after 28 days.
34812488	6	21	theme	bromide	1372:1378	arg1	assay					1399:1403	the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) colorimetric assay	1313:1403	assay	1399:1403	Human BMSC proliferation was assessed using fluorescence imaging, and quantitated via the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) colorimetric assay.
34812488	4	22	theme	mechanical	920:929	arg1	results					986:992	biomaterial dynamic mechanical (DMA), elemental, and thermogravimetric (TGA) analysis results	900:992	basic printing requirements as well as biomaterial dynamic mechanical (DMA), elemental, and thermogravimetric (TGA) analysis results	861:992	In this study, basic printing requirements as well as biomaterial dynamic mechanical (DMA), elemental, and thermogravimetric (TGA) analysis results demonstrate material homogeneity as well as thermal stability.
34812488	7	23	theme	in	1412:1413	arg1	studies					1436:1442	These in vitro cell viability studies	1406:1442	These in vitro cell viability studies	1406:1442	These in vitro cell viability studies revealed that the highest chitosan concentration blend of 20% favoured the most hBMSC growth, exhibited the most swelling, and showed minimal degradation after 28 days.
34812488	5	24	theme	Scaffold	1057:1064	arg1	morphology					1066:1075	Scaffold morphology	1057:1075	Scaffold morphology	1057:1075	Scaffold morphology and microarchitecture were assessed using scanning electron microscopy (SEM) alongside in vitro scaffold degradation and biological characterisation.
34812488	3	25	theme	regeneration	820:831	arg1	application					833:843	potential bone regeneration application	805:843	potential bone regeneration application	805:843	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	7	26	theme	concentration	1479:1491	arg1	blend					1493:1497	the highest chitosan concentration blend	1458:1497	the highest chitosan concentration blend of 20%	1458:1504	These in vitro cell viability studies revealed that the highest chitosan concentration blend of 20% favoured the most hBMSC growth, exhibited the most swelling, and showed minimal degradation after 28 days.
34812488	4	27	theme	analysis	977:984	arg1	results					986:992	biomaterial dynamic mechanical (DMA), elemental, and thermogravimetric (TGA) analysis results	900:992	basic printing requirements as well as biomaterial dynamic mechanical (DMA), elemental, and thermogravimetric (TGA) analysis results	861:992	In this study, basic printing requirements as well as biomaterial dynamic mechanical (DMA), elemental, and thermogravimetric (TGA) analysis results demonstrate material homogeneity as well as thermal stability.
34812488	2	28	used	used	400:403	arg2	poly-caprolactone					371:387	poly-caprolactone	371:387	poly-caprolactone (PCL)	371:393	Towards this end, a commonly used synthetic polyester, poly-caprolactone (PCL), was used to make scaffolds composed of different biomaterial compositions to increase bioactivity using 3D melt pneumatic extrusion technology.
34812488	2	28	used	used	400:403	arg2	polyester					360:368	a commonly used synthetic polyester	334:368	a commonly used synthetic polyester	334:368	Towards this end, a commonly used synthetic polyester, poly-caprolactone (PCL), was used to make scaffolds composed of different biomaterial compositions to increase bioactivity using 3D melt pneumatic extrusion technology.
34812488	3	29	theme	potential	805:813	arg1	application					833:843	potential bone regeneration application	805:843	potential bone regeneration application	805:843	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	0	30	theme	3D	81:82	arg1	scaffolds					108:116	3D melt extruded composite scaffolds	81:116	3D melt extruded composite scaffolds	81:116	A comparison between β-tricalcium phosphate and chitosan poly-caprolactone-based 3D melt extruded composite scaffolds.
34812488	1	31	theme	attractive	160:169	arg1	technology					194:203	an attractive additive manufacturing technology	157:203	an attractive additive manufacturing technology to construct degradable scaffolds as tissue precursors in order to create clinically relevant medical devices	157:313	Melt extrusion 3D printing has become an attractive additive manufacturing technology to construct degradable scaffolds as tissue precursors in order to create clinically relevant medical devices.
34812488	2	32	theme	extrusion	518:526	arg1	technology					528:537	3D melt pneumatic extrusion technology	500:537	3D melt pneumatic extrusion technology	500:537	Towards this end, a commonly used synthetic polyester, poly-caprolactone (PCL), was used to make scaffolds composed of different biomaterial compositions to increase bioactivity using 3D melt pneumatic extrusion technology.
34812488	10	33	theme	potential	2189:2197	arg1	use					2199:2201	their potential use	2183:2201	their potential use for regenerative, bone repair applications	2183:2244	3D melt extruded PCL-based composite scaffolds methodology offers a straightforward way to print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity; and demonstrates their potential use for regenerative, bone repair applications.
34812488	9	34	with	composite	1863:1871	arg1	that					1878:1881	that	1878:1881	that	1878:1881	Overall, this study demonstrates the first direct comparison of a natural biopolymer-based, that is, chitosan, 3D melt extruded PCL composite with that of a bioceramic-based, that is, β-TCP, PCL composite and their effects on hBMSC 3D proliferation.
34812488	0	35	theme	extruded	89:96	arg1	scaffolds					108:116	3D melt extruded composite scaffolds	81:116	3D melt extruded composite scaffolds	81:116	A comparison between β-tricalcium phosphate and chitosan poly-caprolactone-based 3D melt extruded composite scaffolds.
34812488	5	36	theme	in	1164:1165	arg1	degradation					1182:1192	in vitro scaffold degradation	1164:1192	in vitro scaffold degradation	1164:1192	Scaffold morphology and microarchitecture were assessed using scanning electron microscopy (SEM) alongside in vitro scaffold degradation and biological characterisation.
34812488	1	37	theme	manufacturing	180:192	arg1	technology					194:203	an attractive additive manufacturing technology	157:203	an attractive additive manufacturing technology to construct degradable scaffolds as tissue precursors in order to create clinically relevant medical devices	157:313	Melt extrusion 3D printing has become an attractive additive manufacturing technology to construct degradable scaffolds as tissue precursors in order to create clinically relevant medical devices.
34812488	10	38	dep	applications	2233:2244	arg1	repair					2226:2231	bone repair	2221:2231	bone repair	2221:2231	3D melt extruded PCL-based composite scaffolds methodology offers a straightforward way to print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity; and demonstrates their potential use for regenerative, bone repair applications.
34812488	4	39	theme	thermogravimetric	953:969	arg1	results					986:992	biomaterial dynamic mechanical (DMA), elemental, and thermogravimetric (TGA) analysis results	900:992	basic printing requirements as well as biomaterial dynamic mechanical (DMA), elemental, and thermogravimetric (TGA) analysis results	861:992	In this study, basic printing requirements as well as biomaterial dynamic mechanical (DMA), elemental, and thermogravimetric (TGA) analysis results demonstrate material homogeneity as well as thermal stability.
34812488	10	40	theme	scaffolds	2018:2026	arg1	methodology					2028:2038	3D melt extruded PCL-based composite scaffolds methodology	1981:2038	3D melt extruded PCL-based composite scaffolds methodology	1981:2038	3D melt extruded PCL-based composite scaffolds methodology offers a straightforward way to print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity; and demonstrates their potential use for regenerative, bone repair applications.
34812488	10	41	theme	regenerative	2207:2218	arg1	applications					2233:2244	regenerative, bone repair applications	2207:2244	applications	2233:2244	3D melt extruded PCL-based composite scaffolds methodology offers a straightforward way to print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity; and demonstrates their potential use for regenerative, bone repair applications.
34812488	5	42	theme	electron	1128:1135	arg1	SEM					1149:1151	SEM	1149:1151	SEM	1149:1151	Scaffold morphology and microarchitecture were assessed using scanning electron microscopy (SEM) alongside in vitro scaffold degradation and biological characterisation.
34812488	5	42	theme	electron	1128:1135	arg1	microscopy					1137:1146	scanning electron microscopy	1119:1146	scanning electron microscopy (SEM)	1119:1152	Scaffold morphology and microarchitecture were assessed using scanning electron microscopy (SEM) alongside in vitro scaffold degradation and biological characterisation.
34812488	9	43	theme	natural	1797:1803	arg1	biopolymer-based					1805:1820	a natural biopolymer-based	1795:1820	a natural biopolymer-based	1795:1820	Overall, this study demonstrates the first direct comparison of a natural biopolymer-based, that is, chitosan, 3D melt extruded PCL composite with that of a bioceramic-based, that is, β-TCP, PCL composite and their effects on hBMSC 3D proliferation.
34812488	6	44	theme	colorimetric	1386:1397	arg1	assay					1399:1403	the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) colorimetric assay	1313:1403	assay	1399:1403	Human BMSC proliferation was assessed using fluorescence imaging, and quantitated via the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) colorimetric assay.
34812488	4	45	theme	thermal	1038:1044	arg1	stability					1046:1054	thermal stability	1038:1054	material homogeneity as well as thermal stability	1006:1054	In this study, basic printing requirements as well as biomaterial dynamic mechanical (DMA), elemental, and thermogravimetric (TGA) analysis results demonstrate material homogeneity as well as thermal stability.
34812488	10	46	theme	PCL-based	1998:2006	arg1	scaffolds					2018:2026	3D melt extruded PCL-based composite scaffolds	1981:2026	3D melt extruded PCL-based composite scaffolds methodology	1981:2038	3D melt extruded PCL-based composite scaffolds methodology offers a straightforward way to print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity; and demonstrates their potential use for regenerative, bone repair applications.
34812488	2	47	theme	different	435:443	arg1	compositions					457:468	different biomaterial compositions	435:468	different biomaterial compositions	435:468	Towards this end, a commonly used synthetic polyester, poly-caprolactone (PCL), was used to make scaffolds composed of different biomaterial compositions to increase bioactivity using 3D melt pneumatic extrusion technology.
34812488	9	48	theme	3D	1963:1964	arg1	proliferation					1966:1978	hBMSC 3D proliferation	1957:1978	hBMSC 3D proliferation	1957:1978	Overall, this study demonstrates the first direct comparison of a natural biopolymer-based, that is, chitosan, 3D melt extruded PCL composite with that of a bioceramic-based, that is, β-TCP, PCL composite and their effects on hBMSC 3D proliferation.
34812488	1	49	theme	extrusion	124:132	arg1	printing					137:144	Melt extrusion 3D printing	119:144	Melt extrusion 3D printing	119:144	Melt extrusion 3D printing has become an attractive additive manufacturing technology to construct degradable scaffolds as tissue precursors in order to create clinically relevant medical devices.
34812488	2	50	theme	melt	503:506	arg1	technology					528:537	3D melt pneumatic extrusion technology	500:537	3D melt pneumatic extrusion technology	500:537	Towards this end, a commonly used synthetic polyester, poly-caprolactone (PCL), was used to make scaffolds composed of different biomaterial compositions to increase bioactivity using 3D melt pneumatic extrusion technology.
34812488	8	51	theme	degradation	1713:1723	arg1	rate					1725:1728	the fastest degradation rate	1701:1728	the fastest degradation rate	1701:1728	The 20% TCP blend had the second highest hBMSC growth, exhibited moderate swelling, and the fastest degradation rate.
34812488	5	52	theme	scaffold	1173:1180	arg1	degradation					1182:1192	in vitro scaffold degradation	1164:1192	in vitro scaffold degradation	1164:1192	Scaffold morphology and microarchitecture were assessed using scanning electron microscopy (SEM) alongside in vitro scaffold degradation and biological characterisation.
34812488	4	53	theme	material	1006:1013	arg1	homogeneity					1015:1025	material homogeneity	1006:1025	material homogeneity as well as thermal stability	1006:1054	In this study, basic printing requirements as well as biomaterial dynamic mechanical (DMA), elemental, and thermogravimetric (TGA) analysis results demonstrate material homogeneity as well as thermal stability.
34812488	10	54	theme	melt	1984:1987	arg1	scaffolds					2018:2026	3D melt extruded PCL-based composite scaffolds	1981:2026	3D melt extruded PCL-based composite scaffolds methodology	1981:2038	3D melt extruded PCL-based composite scaffolds methodology offers a straightforward way to print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity; and demonstrates their potential use for regenerative, bone repair applications.
34812488	9	55	theme	3D	1842:1843	arg1	PCL					1859:1861	3D melt extruded PCL	1842:1861	3D melt extruded PCL	1842:1861	Overall, this study demonstrates the first direct comparison of a natural biopolymer-based, that is, chitosan, 3D melt extruded PCL composite with that of a bioceramic-based, that is, β-TCP, PCL composite and their effects on hBMSC 3D proliferation.
34812488	9	55	theme	3D	1842:1843	arg1	chitosan					1832:1839	chitosan	1832:1839	chitosan	1832:1839	Overall, this study demonstrates the first direct comparison of a natural biopolymer-based, that is, chitosan, 3D melt extruded PCL composite with that of a bioceramic-based, that is, β-TCP, PCL composite and their effects on hBMSC 3D proliferation.
34812488	9	56	theme	extruded	1850:1857	arg1	PCL					1859:1861	3D melt extruded PCL	1842:1861	3D melt extruded PCL	1842:1861	Overall, this study demonstrates the first direct comparison of a natural biopolymer-based, that is, chitosan, 3D melt extruded PCL composite with that of a bioceramic-based, that is, β-TCP, PCL composite and their effects on hBMSC 3D proliferation.
34812488	9	56	theme	extruded	1850:1857	arg1	chitosan					1832:1839	chitosan	1832:1839	chitosan	1832:1839	Overall, this study demonstrates the first direct comparison of a natural biopolymer-based, that is, chitosan, 3D melt extruded PCL composite with that of a bioceramic-based, that is, β-TCP, PCL composite and their effects on hBMSC 3D proliferation.
34812488	8	57	theme	20	1617:1618	arg1	%					1619:1619	%	1619:1619	%	1619:1619	The 20% TCP blend had the second highest hBMSC growth, exhibited moderate swelling, and the fastest degradation rate.
34812488	3	58	theme	three-dimensional	698:714	arg1	architecture					721:732	three-dimensional (3D) architecture	698:732	three-dimensional (3D) architecture	698:732	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	10	59	theme	straightforward	2049:2063	arg1	way					2065:2067	a straightforward way	2047:2067	a straightforward way to print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity;	2047:2164	3D melt extruded PCL-based composite scaffolds methodology offers a straightforward way to print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity; and demonstrates their potential use for regenerative, bone repair applications.
34812488	8	60	theme	TCP	1621:1623	arg1	blend					1625:1629	The 20% TCP blend	1613:1629	The 20% TCP blend	1613:1629	The 20% TCP blend had the second highest hBMSC growth, exhibited moderate swelling, and the fastest degradation rate.
34812488	8	61	theme	moderate	1678:1685	arg1	swelling					1687:1694	moderate swelling	1678:1694	moderate swelling	1678:1694	The 20% TCP blend had the second highest hBMSC growth, exhibited moderate swelling, and the fastest degradation rate.
34812488	7	62	theme	hBMSC	1524:1528	arg1	growth					1530:1535	the most hBMSC growth	1515:1535	the most hBMSC growth	1515:1535	These in vitro cell viability studies revealed that the highest chitosan concentration blend of 20% favoured the most hBMSC growth, exhibited the most swelling, and showed minimal degradation after 28 days.
34812488	10	63	theme	3D	1981:1982	arg1	scaffolds					2018:2026	3D melt extruded PCL-based composite scaffolds	1981:2026	3D melt extruded PCL-based composite scaffolds methodology	1981:2038	3D melt extruded PCL-based composite scaffolds methodology offers a straightforward way to print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity; and demonstrates their potential use for regenerative, bone repair applications.
34812488	10	64	theme	good	2093:2096	arg1	fidelity					2104:2111	good shape fidelity	2093:2111	good shape fidelity	2093:2111	3D melt extruded PCL-based composite scaffolds methodology offers a straightforward way to print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity; and demonstrates their potential use for regenerative, bone repair applications.
34812488	9	65	theme	direct	1774:1779	arg1	comparison					1781:1790	the first direct comparison	1764:1790	the first direct comparison of a natural biopolymer-based, that is, chitosan, 3D melt extruded PCL composite with that of a bioceramic-based, that is, β-TCP, PCL composite and their effects on hBMSC 3D proliferation	1764:1978	Overall, this study demonstrates the first direct comparison of a natural biopolymer-based, that is, chitosan, 3D melt extruded PCL composite with that of a bioceramic-based, that is, β-TCP, PCL composite and their effects on hBMSC 3D proliferation.
34812488	6	66	theme	5-dimethylthiazol-2-yl	1323:1344	arg1	assay					1399:1403	the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) colorimetric assay	1313:1403	assay	1399:1403	Human BMSC proliferation was assessed using fluorescence imaging, and quantitated via the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) colorimetric assay.
34812488	3	67	theme	Varying	540:546	arg1	ratios					548:553	Varying ratios	540:553	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP)	540:638	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	8	68	theme	second	1639:1644	arg1	growth					1660:1665	the second highest hBMSC growth	1635:1665	the second highest hBMSC growth	1635:1665	The 20% TCP blend had the second highest hBMSC growth, exhibited moderate swelling, and the fastest degradation rate.
34812488	1	69	theme	medical	299:305	arg1	devices					307:313	clinically relevant medical devices	279:313	clinically relevant medical devices	279:313	Melt extrusion 3D printing has become an attractive additive manufacturing technology to construct degradable scaffolds as tissue precursors in order to create clinically relevant medical devices.
34812488	7	70	theme	%	1504:1504	arg1	blend					1493:1497	the highest chitosan concentration blend	1458:1497	the highest chitosan concentration blend of 20%	1458:1504	These in vitro cell viability studies revealed that the highest chitosan concentration blend of 20% favoured the most hBMSC growth, exhibited the most swelling, and showed minimal degradation after 28 days.
34812488	6	71	theme	Human	1227:1231	arg1	proliferation					1238:1250	Human BMSC proliferation	1227:1250	Human BMSC proliferation	1227:1250	Human BMSC proliferation was assessed using fluorescence imaging, and quantitated via the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) colorimetric assay.
34812488	10	72	theme	interconnected	2114:2127	arg1	porosities					2129:2138	interconnected porosities	2114:2138	interconnected porosities	2114:2138	3D melt extruded PCL-based composite scaffolds methodology offers a straightforward way to print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity; and demonstrates their potential use for regenerative, bone repair applications.
34812488	8	73	theme	hBMSC	1654:1658	arg1	growth					1660:1665	the second highest hBMSC growth	1635:1665	the second highest hBMSC growth	1635:1665	The 20% TCP blend had the second highest hBMSC growth, exhibited moderate swelling, and the fastest degradation rate.
34812488	3	74	theme	derived	756:762	arg1	cell					781:784	derived mesenchymal stem cell	756:784	human bone-marrow derived mesenchymal stem cell (hBMSC) growth	738:799	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	3	74	theme	derived	756:762	arg1	hBMSC					787:791	hBMSC	787:791	hBMSC	787:791	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	3	75	theme	natural	562:568	arg1	biopolymer					570:579	the natural biopolymer	558:579	the natural biopolymer	558:579	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	3	76	with	scaffolds	683:691	arg1	architecture					721:732	three-dimensional (3D) architecture	698:732	three-dimensional (3D) architecture	698:732	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	7	77	dep	in	1412:1413	arg1	vitro					1415:1419	vitro	1415:1419	vitro	1415:1419	These in vitro cell viability studies revealed that the highest chitosan concentration blend of 20% favoured the most hBMSC growth, exhibited the most swelling, and showed minimal degradation after 28 days.
34812488	7	78	theme	chitosan	1470:1477	arg1	blend					1493:1497	the highest chitosan concentration blend	1458:1497	the highest chitosan concentration blend of 20%	1458:1504	These in vitro cell viability studies revealed that the highest chitosan concentration blend of 20% favoured the most hBMSC growth, exhibited the most swelling, and showed minimal degradation after 28 days.
34812488	3	79	theme	human	738:742	arg1	growth					794:799	human bone-marrow derived mesenchymal stem cell (hBMSC) growth	738:799	human bone-marrow derived mesenchymal stem cell (hBMSC) growth	738:799	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	2	80	theme	used	345:348	arg1	poly-caprolactone					371:387	poly-caprolactone	371:387	poly-caprolactone (PCL)	371:393	Towards this end, a commonly used synthetic polyester, poly-caprolactone (PCL), was used to make scaffolds composed of different biomaterial compositions to increase bioactivity using 3D melt pneumatic extrusion technology.
34812488	2	80	theme	used	345:348	arg1	polyester					360:368	a commonly used synthetic polyester	334:368	a commonly used synthetic polyester	334:368	Towards this end, a commonly used synthetic polyester, poly-caprolactone (PCL), was used to make scaffolds composed of different biomaterial compositions to increase bioactivity using 3D melt pneumatic extrusion technology.
34812488	4	81	theme	printing	867:874	arg1	requirements					876:887	basic printing requirements	861:887	basic printing requirements as well as biomaterial dynamic mechanical (DMA), elemental, and thermogravimetric (TGA) analysis results	861:992	In this study, basic printing requirements as well as biomaterial dynamic mechanical (DMA), elemental, and thermogravimetric (TGA) analysis results demonstrate material homogeneity as well as thermal stability.
34812488	3	82	dep	bioceramic	599:608	arg1	β-tricalcium					611:622	β-tricalcium	611:622	β-tricalcium	611:622	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	9	83	theme	PCL	1922:1924	arg1	composite					1926:1934	PCL composite	1922:1934	PCL composite	1922:1934	Overall, this study demonstrates the first direct comparison of a natural biopolymer-based, that is, chitosan, 3D melt extruded PCL composite with that of a bioceramic-based, that is, β-TCP, PCL composite and their effects on hBMSC 3D proliferation.
34812488	3	84	theme	stem	776:779	arg1	cell					781:784	derived mesenchymal stem cell	756:784	human bone-marrow derived mesenchymal stem cell (hBMSC) growth	738:799	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	3	84	theme	stem	776:779	arg1	hBMSC					787:791	hBMSC	787:791	hBMSC	787:791	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	3	85	theme	phosphate	624:632	arg1	ratios					548:553	Varying ratios	540:553	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP)	540:638	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	1	86	theme	tissue	242:247	arg1	scaffolds					229:237	degradable scaffolds	218:237	degradable scaffolds	218:237	Melt extrusion 3D printing has become an attractive additive manufacturing technology to construct degradable scaffolds as tissue precursors in order to create clinically relevant medical devices.
34812488	1	86	theme	tissue	242:247	arg1	precursors					249:258	tissue precursors	242:258	tissue precursors	242:258	Melt extrusion 3D printing has become an attractive additive manufacturing technology to construct degradable scaffolds as tissue precursors in order to create clinically relevant medical devices.
34812488	4	87	theme	elemental	938:946	arg1	results					986:992	biomaterial dynamic mechanical (DMA), elemental, and thermogravimetric (TGA) analysis results	900:992	basic printing requirements as well as biomaterial dynamic mechanical (DMA), elemental, and thermogravimetric (TGA) analysis results	861:992	In this study, basic printing requirements as well as biomaterial dynamic mechanical (DMA), elemental, and thermogravimetric (TGA) analysis results demonstrate material homogeneity as well as thermal stability.
34812488	7	88	theme	viability	1426:1434	arg1	studies					1436:1442	These in vitro cell viability studies	1406:1442	These in vitro cell viability studies	1406:1442	These in vitro cell viability studies revealed that the highest chitosan concentration blend of 20% favoured the most hBMSC growth, exhibited the most swelling, and showed minimal degradation after 28 days.
34812488	10	89	with	scaffolds	2078:2086	arg1	fidelity					2104:2111	good shape fidelity	2093:2111	good shape fidelity	2093:2111	3D melt extruded PCL-based composite scaffolds methodology offers a straightforward way to print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity; and demonstrates their potential use for regenerative, bone repair applications.
34812488	10	89	with	scaffolds	2078:2086	arg1	porosities					2129:2138	interconnected porosities	2114:2138	interconnected porosities	2114:2138	3D melt extruded PCL-based composite scaffolds methodology offers a straightforward way to print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity; and demonstrates their potential use for regenerative, bone repair applications.
34812488	10	89	with	scaffolds	2078:2086	arg1	bioactivity					2153:2163	enhanced bioactivity	2144:2163	enhanced bioactivity;	2144:2164	3D melt extruded PCL-based composite scaffolds methodology offers a straightforward way to print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity; and demonstrates their potential use for regenerative, bone repair applications.
34812488	5	90	theme	scanning	1119:1126	arg1	SEM					1149:1151	SEM	1149:1151	SEM	1149:1151	Scaffold morphology and microarchitecture were assessed using scanning electron microscopy (SEM) alongside in vitro scaffold degradation and biological characterisation.
34812488	5	90	theme	scanning	1119:1126	arg1	microscopy					1137:1146	scanning electron microscopy	1119:1146	scanning electron microscopy (SEM)	1119:1152	Scaffold morphology and microarchitecture were assessed using scanning electron microscopy (SEM) alongside in vitro scaffold degradation and biological characterisation.
34812488	1	91	theme	3D	134:135	arg1	printing					137:144	Melt extrusion 3D printing	119:144	Melt extrusion 3D printing	119:144	Melt extrusion 3D printing has become an attractive additive manufacturing technology to construct degradable scaffolds as tissue precursors in order to create clinically relevant medical devices.
34812488	6	92	theme	fluorescence	1271:1282	arg1	imaging					1284:1290	fluorescence imaging	1271:1290	fluorescence imaging	1271:1290	Human BMSC proliferation was assessed using fluorescence imaging, and quantitated via the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) colorimetric assay.
34812488	9	93	theme	hBMSC	1957:1961	arg1	proliferation					1966:1978	hBMSC 3D proliferation	1957:1978	hBMSC 3D proliferation	1957:1978	Overall, this study demonstrates the first direct comparison of a natural biopolymer-based, that is, chitosan, 3D melt extruded PCL composite with that of a bioceramic-based, that is, β-TCP, PCL composite and their effects on hBMSC 3D proliferation.
34812488	10	94	theme	extruded	1989:1996	arg1	scaffolds					2018:2026	3D melt extruded PCL-based composite scaffolds	1981:2026	3D melt extruded PCL-based composite scaffolds methodology	1981:2038	3D melt extruded PCL-based composite scaffolds methodology offers a straightforward way to print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity; and demonstrates their potential use for regenerative, bone repair applications.
34812488	0	95	theme	melt	84:87	arg1	scaffolds					108:116	3D melt extruded composite scaffolds	81:116	3D melt extruded composite scaffolds	81:116	A comparison between β-tricalcium phosphate and chitosan poly-caprolactone-based 3D melt extruded composite scaffolds.
34812488	9	96	theme	first	1768:1772	arg1	comparison					1781:1790	the first direct comparison	1764:1790	the first direct comparison of a natural biopolymer-based, that is, chitosan, 3D melt extruded PCL composite with that of a bioceramic-based, that is, β-TCP, PCL composite and their effects on hBMSC 3D proliferation	1764:1978	Overall, this study demonstrates the first direct comparison of a natural biopolymer-based, that is, chitosan, 3D melt extruded PCL composite with that of a bioceramic-based, that is, β-TCP, PCL composite and their effects on hBMSC 3D proliferation.
34812488	3	97	theme	bone	815:818	arg1	application					833:843	potential bone regeneration application	805:843	potential bone regeneration application	805:843	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	0	98	theme	composite	98:106	arg1	scaffolds					108:116	3D melt extruded composite scaffolds	81:116	3D melt extruded composite scaffolds	81:116	A comparison between β-tricalcium phosphate and chitosan poly-caprolactone-based 3D melt extruded composite scaffolds.
34812488	6	99	dep	assay	1399:1403	arg1	5-diphenyltetrazolium					1350:1370	the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) colorimetric assay	1313:1403	5-diphenyltetrazolium	1350:1370	Human BMSC proliferation was assessed using fluorescence imaging, and quantitated via the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) colorimetric assay.
34812488	1	100	theme	additive	171:178	arg1	technology					194:203	an attractive additive manufacturing technology	157:203	an attractive additive manufacturing technology to construct degradable scaffolds as tissue precursors in order to create clinically relevant medical devices	157:313	Melt extrusion 3D printing has become an attractive additive manufacturing technology to construct degradable scaffolds as tissue precursors in order to create clinically relevant medical devices.
34812488	2	101	theme	pneumatic	508:516	arg1	technology					528:537	3D melt pneumatic extrusion technology	500:537	3D melt pneumatic extrusion technology	500:537	Towards this end, a commonly used synthetic polyester, poly-caprolactone (PCL), was used to make scaffolds composed of different biomaterial compositions to increase bioactivity using 3D melt pneumatic extrusion technology.
34812488	7	102	theme	minimal	1578:1584	arg1	degradation					1586:1596	minimal degradation	1578:1596	minimal degradation	1578:1596	These in vitro cell viability studies revealed that the highest chitosan concentration blend of 20% favoured the most hBMSC growth, exhibited the most swelling, and showed minimal degradation after 28 days.
34812488	10	103	theme	bone	2221:2224	arg1	repair					2226:2231	bone repair	2221:2231	bone repair	2221:2231	3D melt extruded PCL-based composite scaffolds methodology offers a straightforward way to print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity; and demonstrates their potential use for regenerative, bone repair applications.
34812488	10	104	theme	shape	2098:2102	arg1	fidelity					2104:2111	good shape fidelity	2093:2111	good shape fidelity	2093:2111	3D melt extruded PCL-based composite scaffolds methodology offers a straightforward way to print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity; and demonstrates their potential use for regenerative, bone repair applications.
34812488	10	105	theme	composite	2008:2016	arg1	scaffolds					2018:2026	3D melt extruded PCL-based composite scaffolds	1981:2026	3D melt extruded PCL-based composite scaffolds methodology	1981:2038	3D melt extruded PCL-based composite scaffolds methodology offers a straightforward way to print scaffolds with good shape fidelity, interconnected porosities and enhanced bioactivity; and demonstrates their potential use for regenerative, bone repair applications.
34812488	8	106	dep	had	1631:1633	arg1	exhibited					1668:1676	exhibited	1668:1676	exhibited moderate swelling, and the fastest degradation rate	1668:1728	The 20% TCP blend had the second highest hBMSC growth, exhibited moderate swelling, and the fastest degradation rate.
34812488	9	107	theme	biopolymer-based	1805:1820	arg1	comparison					1781:1790	the first direct comparison	1764:1790	the first direct comparison of a natural biopolymer-based, that is, chitosan, 3D melt extruded PCL composite with that of a bioceramic-based, that is, β-TCP, PCL composite and their effects on hBMSC 3D proliferation	1764:1978	Overall, this study demonstrates the first direct comparison of a natural biopolymer-based, that is, chitosan, 3D melt extruded PCL composite with that of a bioceramic-based, that is, β-TCP, PCL composite and their effects on hBMSC 3D proliferation.
34812488	8	108	contain	had	1631:1633	arg2	growth					1660:1665	the second highest hBMSC growth	1635:1665	the second highest hBMSC growth	1635:1665	The 20% TCP blend had the second highest hBMSC growth, exhibited moderate swelling, and the fastest degradation rate.
34812488	8	108	contain	had	1631:1633	arg1	blend					1625:1629	The 20% TCP blend	1613:1629	The 20% TCP blend	1613:1629	The 20% TCP blend had the second highest hBMSC growth, exhibited moderate swelling, and the fastest degradation rate.
34812488	5	109	dep	in	1164:1165	arg1	vitro					1167:1171	vitro	1167:1171	vitro	1167:1171	Scaffold morphology and microarchitecture were assessed using scanning electron microscopy (SEM) alongside in vitro scaffold degradation and biological characterisation.
34812488	0	110	theme	β-tricalcium	21:32	arg1	phosphate					34:42	β-tricalcium phosphate	21:42	β-tricalcium phosphate	21:42	A comparison between β-tricalcium phosphate and chitosan poly-caprolactone-based 3D melt extruded composite scaffolds.
34812488	1	111	theme	Melt	119:122	arg1	printing					137:144	Melt extrusion 3D printing	119:144	Melt extrusion 3D printing	119:144	Melt extrusion 3D printing has become an attractive additive manufacturing technology to construct degradable scaffolds as tissue precursors in order to create clinically relevant medical devices.
34812488	9	112	from	effects	1946:1952	arg1	proliferation					1966:1978	hBMSC 3D proliferation	1957:1978	hBMSC 3D proliferation	1957:1978	Overall, this study demonstrates the first direct comparison of a natural biopolymer-based, that is, chitosan, 3D melt extruded PCL composite with that of a bioceramic-based, that is, β-TCP, PCL composite and their effects on hBMSC 3D proliferation.
34812488	2	113	theme	biomaterial	445:455	arg1	compositions					457:468	different biomaterial compositions	435:468	different biomaterial compositions	435:468	Towards this end, a commonly used synthetic polyester, poly-caprolactone (PCL), was used to make scaffolds composed of different biomaterial compositions to increase bioactivity using 3D melt pneumatic extrusion technology.
34812488	2	114	theme	3D	500:501	arg1	technology					528:537	3D melt pneumatic extrusion technology	500:537	3D melt pneumatic extrusion technology	500:537	Towards this end, a commonly used synthetic polyester, poly-caprolactone (PCL), was used to make scaffolds composed of different biomaterial compositions to increase bioactivity using 3D melt pneumatic extrusion technology.
34812488	9	115	theme	melt	1845:1848	arg1	PCL					1859:1861	3D melt extruded PCL	1842:1861	3D melt extruded PCL	1842:1861	Overall, this study demonstrates the first direct comparison of a natural biopolymer-based, that is, chitosan, 3D melt extruded PCL composite with that of a bioceramic-based, that is, β-TCP, PCL composite and their effects on hBMSC 3D proliferation.
34812488	9	115	theme	melt	1845:1848	arg1	chitosan					1832:1839	chitosan	1832:1839	chitosan	1832:1839	Overall, this study demonstrates the first direct comparison of a natural biopolymer-based, that is, chitosan, 3D melt extruded PCL composite with that of a bioceramic-based, that is, β-TCP, PCL composite and their effects on hBMSC 3D proliferation.
34812488	8	116	theme	%	1619:1619	arg1	blend					1625:1629	The 20% TCP blend	1613:1629	The 20% TCP blend	1613:1629	The 20% TCP blend had the second highest hBMSC growth, exhibited moderate swelling, and the fastest degradation rate.
34812488	5	117	theme	biological	1198:1207	arg1	characterisation					1209:1224	biological characterisation	1198:1224	biological characterisation	1198:1224	Scaffold morphology and microarchitecture were assessed using scanning electron microscopy (SEM) alongside in vitro scaffold degradation and biological characterisation.
34812488	7	118	theme	most	1552:1555	arg1	swelling					1557:1564	the most swelling	1548:1564	the most swelling	1548:1564	These in vitro cell viability studies revealed that the highest chitosan concentration blend of 20% favoured the most hBMSC growth, exhibited the most swelling, and showed minimal degradation after 28 days.
34812488	9	119	from	composite	1926:1934	arg1	proliferation					1966:1978	hBMSC 3D proliferation	1957:1978	hBMSC 3D proliferation	1957:1978	Overall, this study demonstrates the first direct comparison of a natural biopolymer-based, that is, chitosan, 3D melt extruded PCL composite with that of a bioceramic-based, that is, β-TCP, PCL composite and their effects on hBMSC 3D proliferation.
34812488	3	120	theme	support	675:681	arg1	scaffolds					683:691	support scaffolds	675:691	support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth	675:799	Varying ratios of the natural biopolymer, chitosan, or the bioceramic, β-tricalcium phosphate (TCP) were blended with PCL to fabricate support scaffolds with three-dimensional (3D) architecture for human bone-marrow derived mesenchymal stem cell (hBMSC) growth for potential bone regeneration application.
34812488	2	121	theme	synthetic	350:358	arg1	poly-caprolactone					371:387	poly-caprolactone	371:387	poly-caprolactone (PCL)	371:393	Towards this end, a commonly used synthetic polyester, poly-caprolactone (PCL), was used to make scaffolds composed of different biomaterial compositions to increase bioactivity using 3D melt pneumatic extrusion technology.
34812488	2	121	theme	synthetic	350:358	arg1	polyester					360:368	a commonly used synthetic polyester	334:368	a commonly used synthetic polyester	334:368	Towards this end, a commonly used synthetic polyester, poly-caprolactone (PCL), was used to make scaffolds composed of different biomaterial compositions to increase bioactivity using 3D melt pneumatic extrusion technology.
34794805	0	0	theme	improved	119:126	arg1	retardancy					134:143	improved flame retardancy	119:143	improved flame retardancy	119:143	Self-assembled supramolecule for synthesizing highly thermally conductive Cellulose/Carbon nitride nanocomposites with improved flame retardancy.
34794805	4	1	theme	K-1	854:856	arg1	conductivity					819:830	remarkable in-plane thermal conductivity	791:830	remarkable in-plane thermal conductivity of 11.83 ± 0.41 W m-1 K-1	791:856	Consequently, the resultant CNF/SCN composites exhibited remarkable in-plane thermal conductivity of 11.83 ± 0.41 W m-1 K-1 at 30 wt% SCN content, which was attributed to the significantly reduced interfacial phonon scattering.
34794805	1	2	theme	thermal	199:205	arg1	conductivity					207:218	excellent thermal conductivity	189:218	excellent thermal conductivity	189:218	The fabrication of polymer composites with excellent thermal conductivity typically involves complex matrix or fillers modifications.
34794805	6	3	theme	robust	1433:1438	arg1	residual					1408:1415	residual	1408:1415	residual	1408:1415	Further analysis of char residuals revealed a relatively dense surface, high concentration of carbon materials, and a high degree of graphitization, indicating that the char residual functioned as a robust physical barrier to effectively inhibit combustion.
34794805	6	3	theme	robust	1433:1438	arg1	barrier					1449:1455	a robust physical barrier	1431:1455	a robust physical barrier to effectively inhibit combustion	1431:1489	Further analysis of char residuals revealed a relatively dense surface, high concentration of carbon materials, and a high degree of graphitization, indicating that the char residual functioned as a robust physical barrier to effectively inhibit combustion.
34794805	7	4	theme	flame	1600:1604	arg1	retardancy					1606:1615	flame retardancy	1600:1615	flame retardancy	1600:1615	This study provides a facile approach to achieving high-efficiency improvements in thermal conductivity and flame retardancy, and simultaneously facilitating broader applications of carbon nitride in thermal management.
34794805	6	5	theme	high	1352:1355	arg1	degree					1357:1362	a high degree	1350:1362	a high degree of graphitization	1350:1380	Further analysis of char residuals revealed a relatively dense surface, high concentration of carbon materials, and a high degree of graphitization, indicating that the char residual functioned as a robust physical barrier to effectively inhibit combustion.
34794805	2	6	theme	/supramolecule-synthesized	415:440	arg1	composites					463:472	highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites	362:472	highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites	362:472	This study proposed a simple technique based on precursor selection for obtaining highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites.
34794805	4	7	theme	phonon	943:948	arg1	scattering					950:959	the significantly reduced interfacial phonon scattering	905:959	the significantly reduced interfacial phonon scattering	905:959	Consequently, the resultant CNF/SCN composites exhibited remarkable in-plane thermal conductivity of 11.83 ± 0.41 W m-1 K-1 at 30 wt% SCN content, which was attributed to the significantly reduced interfacial phonon scattering.
34794805	5	8	theme	peak	1107:1110	arg1	rate					1125:1128	peak heat release rate	1107:1128	peak heat release rate	1107:1128	It also showed evident improvements in electrical insulation and flame retardancy compared with the pure CNF film, where the volume resistivity, peak heat release rate, and total heat release were remarkably enhanced by 1242% and reduced by 59.9% and 15.8%, respectively.
34794805	7	9	from	improvements	1559:1570	arg1	conductivity					1583:1594	thermal conductivity	1575:1594	thermal conductivity	1575:1594	This study provides a facile approach to achieving high-efficiency improvements in thermal conductivity and flame retardancy, and simultaneously facilitating broader applications of carbon nitride in thermal management.
34794805	7	9	from	improvements	1559:1570	arg1	retardancy					1606:1615	flame retardancy	1600:1615	flame retardancy	1600:1615	This study provides a facile approach to achieving high-efficiency improvements in thermal conductivity and flame retardancy, and simultaneously facilitating broader applications of carbon nitride in thermal management.
34794805	3	10	theme	Fourier-transform	475:491	arg1	tests					502:506	Fourier-transform infrared tests	475:506	Fourier-transform infrared tests	475:506	Fourier-transform infrared tests demonstrated the construction of hydrogen bonds between CNF and SCN; a highly ordered structure and relatively compact in-plane stacking were confirmed via scanning electron microscopy and X-ray diffraction characterizations.
34794805	0	11	theme	flame	128:132	arg1	retardancy					134:143	improved flame retardancy	119:143	improved flame retardancy	119:143	Self-assembled supramolecule for synthesizing highly thermally conductive Cellulose/Carbon nitride nanocomposites with improved flame retardancy.
34794805	7	12	theme	carbon	1674:1679	arg1	nitride					1681:1687	carbon nitride	1674:1687	carbon nitride	1674:1687	This study provides a facile approach to achieving high-efficiency improvements in thermal conductivity and flame retardancy, and simultaneously facilitating broader applications of carbon nitride in thermal management.
34794805	5	13	theme	heat	1112:1115	arg1	rate					1125:1128	peak heat release rate	1107:1128	peak heat release rate	1107:1128	It also showed evident improvements in electrical insulation and flame retardancy compared with the pure CNF film, where the volume resistivity, peak heat release rate, and total heat release were remarkably enhanced by 1242% and reduced by 59.9% and 15.8%, respectively.
34794805	6	14	theme	graphitization	1367:1380	arg1	concentration					1311:1323	high concentration	1306:1323	high concentration of carbon materials	1306:1343	Further analysis of char residuals revealed a relatively dense surface, high concentration of carbon materials, and a high degree of graphitization, indicating that the char residual functioned as a robust physical barrier to effectively inhibit combustion.
34794805	6	14	theme	graphitization	1367:1380	arg1	degree					1357:1362	a high degree	1350:1362	a high degree of graphitization	1350:1380	Further analysis of char residuals revealed a relatively dense surface, high concentration of carbon materials, and a high degree of graphitization, indicating that the char residual functioned as a robust physical barrier to effectively inhibit combustion.
34794805	6	14	theme	graphitization	1367:1380	arg1	surface					1297:1303	a relatively dense surface	1278:1303	a relatively dense surface	1278:1303	Further analysis of char residuals revealed a relatively dense surface, high concentration of carbon materials, and a high degree of graphitization, indicating that the char residual functioned as a robust physical barrier to effectively inhibit combustion.
34794805	2	15	theme	carbon	442:447	arg1	composites					463:472	highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites	362:472	highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites	362:472	This study proposed a simple technique based on precursor selection for obtaining highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites.
34794805	2	16	theme	nanofiber	400:408	arg1	composites					463:472	highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites	362:472	highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites	362:472	This study proposed a simple technique based on precursor selection for obtaining highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites.
34794805	5	17	theme	CNF	1067:1069	arg1	film					1071:1074	the pure CNF film	1058:1074	the pure CNF film	1058:1074	It also showed evident improvements in electrical insulation and flame retardancy compared with the pure CNF film, where the volume resistivity, peak heat release rate, and total heat release were remarkably enhanced by 1242% and reduced by 59.9% and 15.8%, respectively.
34794805	3	18	theme	compact	619:625	arg1	stacking					636:643	relatively compact in-plane stacking	608:643	relatively compact in-plane stacking	608:643	Fourier-transform infrared tests demonstrated the construction of hydrogen bonds between CNF and SCN; a highly ordered structure and relatively compact in-plane stacking were confirmed via scanning electron microscopy and X-ray diffraction characterizations.
34794805	4	19	theme	thermal	811:817	arg1	conductivity					819:830	remarkable in-plane thermal conductivity	791:830	remarkable in-plane thermal conductivity of 11.83 ± 0.41 W m-1 K-1	791:856	Consequently, the resultant CNF/SCN composites exhibited remarkable in-plane thermal conductivity of 11.83 ± 0.41 W m-1 K-1 at 30 wt% SCN content, which was attributed to the significantly reduced interfacial phonon scattering.
34794805	3	20	dep	microscopy	682:691	arg1	characterizations					715:731	characterizations	715:731	characterizations	715:731	Fourier-transform infrared tests demonstrated the construction of hydrogen bonds between CNF and SCN; a highly ordered structure and relatively compact in-plane stacking were confirmed via scanning electron microscopy and X-ray diffraction characterizations.
34794805	3	21	theme	infrared	493:500	arg1	tests					502:506	Fourier-transform infrared tests	475:506	Fourier-transform infrared tests	475:506	Fourier-transform infrared tests demonstrated the construction of hydrogen bonds between CNF and SCN; a highly ordered structure and relatively compact in-plane stacking were confirmed via scanning electron microscopy and X-ray diffraction characterizations.
34794805	5	22	theme	pure	1062:1065	arg1	film					1071:1074	the pure CNF film	1058:1074	the pure CNF film	1058:1074	It also showed evident improvements in electrical insulation and flame retardancy compared with the pure CNF film, where the volume resistivity, peak heat release rate, and total heat release were remarkably enhanced by 1242% and reduced by 59.9% and 15.8%, respectively.
34794805	2	23	theme	precursor	328:336	arg1	selection					338:346	precursor selection	328:346	precursor selection for obtaining highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites	328:472	This study proposed a simple technique based on precursor selection for obtaining highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites.
34794805	3	24	theme	hydrogen	541:548	arg1	bonds					550:554	hydrogen bonds	541:554	hydrogen bonds	541:554	Fourier-transform infrared tests demonstrated the construction of hydrogen bonds between CNF and SCN; a highly ordered structure and relatively compact in-plane stacking were confirmed via scanning electron microscopy and X-ray diffraction characterizations.
34794805	7	25	theme	thermal	1692:1698	arg1	management					1700:1709	thermal management	1692:1709	thermal management	1692:1709	This study provides a facile approach to achieving high-efficiency improvements in thermal conductivity and flame retardancy, and simultaneously facilitating broader applications of carbon nitride in thermal management.
34794805	7	26	theme	thermal	1575:1581	arg1	conductivity					1583:1594	thermal conductivity	1575:1594	thermal conductivity	1575:1594	This study provides a facile approach to achieving high-efficiency improvements in thermal conductivity and flame retardancy, and simultaneously facilitating broader applications of carbon nitride in thermal management.
34794805	1	27	with	fabrication	150:160	arg1	conductivity					207:218	excellent thermal conductivity	189:218	excellent thermal conductivity	189:218	The fabrication of polymer composites with excellent thermal conductivity typically involves complex matrix or fillers modifications.
34794805	2	28	theme	cellulose	390:398	arg1	composites					463:472	highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites	362:472	highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites	362:472	This study proposed a simple technique based on precursor selection for obtaining highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites.
34794805	1	29	theme	complex	239:245	arg1	matrix					247:252	complex matrix	239:252	complex matrix	239:252	The fabrication of polymer composites with excellent thermal conductivity typically involves complex matrix or fillers modifications.
34794805	7	30	theme	high-efficiency	1543:1557	arg1	improvements					1559:1570	high-efficiency improvements	1543:1570	high-efficiency improvements in thermal conductivity and flame retardancy	1543:1615	This study provides a facile approach to achieving high-efficiency improvements in thermal conductivity and flame retardancy, and simultaneously facilitating broader applications of carbon nitride in thermal management.
34794805	4	31	theme	%	866:866	arg1	content					872:878	30 wt% SCN content	861:878	30 wt% SCN content	861:878	Consequently, the resultant CNF/SCN composites exhibited remarkable in-plane thermal conductivity of 11.83 ± 0.41 W m-1 K-1 at 30 wt% SCN content, which was attributed to the significantly reduced interfacial phonon scattering.
34794805	2	32	theme	conductive	379:388	arg1	composites					463:472	highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites	362:472	highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites	362:472	This study proposed a simple technique based on precursor selection for obtaining highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites.
34794805	2	33	theme	SCN	458:460	arg1	composites					463:472	highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites	362:472	highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites	362:472	This study proposed a simple technique based on precursor selection for obtaining highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites.
34794805	6	34	theme	high	1306:1309	arg1	concentration					1311:1323	high concentration	1306:1323	high concentration of carbon materials	1306:1343	Further analysis of char residuals revealed a relatively dense surface, high concentration of carbon materials, and a high degree of graphitization, indicating that the char residual functioned as a robust physical barrier to effectively inhibit combustion.
34794805	6	35	theme	physical	1440:1447	arg1	residual					1408:1415	residual	1408:1415	residual	1408:1415	Further analysis of char residuals revealed a relatively dense surface, high concentration of carbon materials, and a high degree of graphitization, indicating that the char residual functioned as a robust physical barrier to effectively inhibit combustion.
34794805	6	35	theme	physical	1440:1447	arg1	barrier					1449:1455	a robust physical barrier	1431:1455	a robust physical barrier to effectively inhibit combustion	1431:1489	Further analysis of char residuals revealed a relatively dense surface, high concentration of carbon materials, and a high degree of graphitization, indicating that the char residual functioned as a robust physical barrier to effectively inhibit combustion.
34794805	6	36	theme	carbon	1328:1333	arg1	materials					1335:1343	carbon materials	1328:1343	carbon materials	1328:1343	Further analysis of char residuals revealed a relatively dense surface, high concentration of carbon materials, and a high degree of graphitization, indicating that the char residual functioned as a robust physical barrier to effectively inhibit combustion.
34794805	6	37	theme	char	1254:1257	arg1	residuals					1259:1267	char residuals	1254:1267	char residuals	1254:1267	Further analysis of char residuals revealed a relatively dense surface, high concentration of carbon materials, and a high degree of graphitization, indicating that the char residual functioned as a robust physical barrier to effectively inhibit combustion.
34794805	4	38	theme	resultant	752:760	arg1	composites					770:779	the resultant CNF/SCN composites	748:779	the resultant CNF/SCN composites	748:779	Consequently, the resultant CNF/SCN composites exhibited remarkable in-plane thermal conductivity of 11.83 ± 0.41 W m-1 K-1 at 30 wt% SCN content, which was attributed to the significantly reduced interfacial phonon scattering.
34794805	3	39	theme	electron	673:680	arg1	microscopy					682:691	scanning electron microscopy	664:691	scanning electron microscopy	664:691	Fourier-transform infrared tests demonstrated the construction of hydrogen bonds between CNF and SCN; a highly ordered structure and relatively compact in-plane stacking were confirmed via scanning electron microscopy and X-ray diffraction characterizations.
34794805	5	40	theme	volume	1087:1092	arg1	resistivity					1094:1104	the volume resistivity	1083:1104	the volume resistivity	1083:1104	It also showed evident improvements in electrical insulation and flame retardancy compared with the pure CNF film, where the volume resistivity, peak heat release rate, and total heat release were remarkably enhanced by 1242% and reduced by 59.9% and 15.8%, respectively.
34794805	7	41	theme	nitride	1681:1687	arg1	applications					1658:1669	broader applications	1650:1669	broader applications of carbon nitride in thermal management	1650:1709	This study provides a facile approach to achieving high-efficiency improvements in thermal conductivity and flame retardancy, and simultaneously facilitating broader applications of carbon nitride in thermal management.
34794805	4	42	theme	11.83 ± 0.41 W m-1	835:852	arg1	K-1					854:856	11.83 ± 0.41 W m-1 K-1	835:856	11.83 ± 0.41 W m-1 K-1	835:856	Consequently, the resultant CNF/SCN composites exhibited remarkable in-plane thermal conductivity of 11.83 ± 0.41 W m-1 K-1 at 30 wt% SCN content, which was attributed to the significantly reduced interfacial phonon scattering.
34794805	7	43	theme	facile	1514:1519	arg1	approach					1521:1528	a facile approach	1512:1528	a facile approach to achieving high-efficiency improvements in thermal conductivity and flame retardancy, and simultaneously facilitating broader applications of carbon nitride in thermal management	1512:1709	This study provides a facile approach to achieving high-efficiency improvements in thermal conductivity and flame retardancy, and simultaneously facilitating broader applications of carbon nitride in thermal management.
34794805	6	44	theme	residuals	1259:1267	arg1	analysis					1242:1249	Further analysis	1234:1249	Further analysis of char residuals	1234:1267	Further analysis of char residuals revealed a relatively dense surface, high concentration of carbon materials, and a high degree of graphitization, indicating that the char residual functioned as a robust physical barrier to effectively inhibit combustion.
34794805	3	45	theme	ordered	586:592	arg1	structure					594:602	a highly ordered structure	577:602	a highly ordered structure	577:602	Fourier-transform infrared tests demonstrated the construction of hydrogen bonds between CNF and SCN; a highly ordered structure and relatively compact in-plane stacking were confirmed via scanning electron microscopy and X-ray diffraction characterizations.
34794805	5	46	theme	heat	1141:1144	arg1	release					1146:1152	total heat release	1135:1152	total heat release	1135:1152	It also showed evident improvements in electrical insulation and flame retardancy compared with the pure CNF film, where the volume resistivity, peak heat release rate, and total heat release were remarkably enhanced by 1242% and reduced by 59.9% and 15.8%, respectively.
34794805	6	47	theme	Further	1234:1240	arg1	analysis					1242:1249	Further analysis	1234:1249	Further analysis of char residuals	1234:1267	Further analysis of char residuals revealed a relatively dense surface, high concentration of carbon materials, and a high degree of graphitization, indicating that the char residual functioned as a robust physical barrier to effectively inhibit combustion.
34794805	3	48	theme	X-ray	697:701	arg1	diffraction					703:713	X-ray diffraction	697:713	X-ray diffraction	697:713	Fourier-transform infrared tests demonstrated the construction of hydrogen bonds between CNF and SCN; a highly ordered structure and relatively compact in-plane stacking were confirmed via scanning electron microscopy and X-ray diffraction characterizations.
34794805	4	49	theme	in-plane	802:809	arg1	conductivity					819:830	remarkable in-plane thermal conductivity	791:830	remarkable in-plane thermal conductivity of 11.83 ± 0.41 W m-1 K-1	791:856	Consequently, the resultant CNF/SCN composites exhibited remarkable in-plane thermal conductivity of 11.83 ± 0.41 W m-1 K-1 at 30 wt% SCN content, which was attributed to the significantly reduced interfacial phonon scattering.
34794805	3	50	theme	bonds	550:554	arg1	construction					525:536	the construction	521:536	the construction of hydrogen bonds between CNF and SCN	521:574	Fourier-transform infrared tests demonstrated the construction of hydrogen bonds between CNF and SCN; a highly ordered structure and relatively compact in-plane stacking were confirmed via scanning electron microscopy and X-ray diffraction characterizations.
34794805	4	51	theme	remarkable	791:800	arg1	conductivity					819:830	remarkable in-plane thermal conductivity	791:830	remarkable in-plane thermal conductivity of 11.83 ± 0.41 W m-1 K-1	791:856	Consequently, the resultant CNF/SCN composites exhibited remarkable in-plane thermal conductivity of 11.83 ± 0.41 W m-1 K-1 at 30 wt% SCN content, which was attributed to the significantly reduced interfacial phonon scattering.
34794805	5	52	theme	flame	1027:1031	arg1	retardancy					1033:1042	flame retardancy	1027:1042	flame retardancy	1027:1042	It also showed evident improvements in electrical insulation and flame retardancy compared with the pure CNF film, where the volume resistivity, peak heat release rate, and total heat release were remarkably enhanced by 1242% and reduced by 59.9% and 15.8%, respectively.
34794805	1	53	dep	matrix	247:252	arg1	modifications					265:277	modifications	265:277	modifications	265:277	The fabrication of polymer composites with excellent thermal conductivity typically involves complex matrix or fillers modifications.
34794805	5	54	theme	electrical	1001:1010	arg1	insulation					1012:1021	electrical insulation	1001:1021	electrical insulation	1001:1021	It also showed evident improvements in electrical insulation and flame retardancy compared with the pure CNF film, where the volume resistivity, peak heat release rate, and total heat release were remarkably enhanced by 1242% and reduced by 59.9% and 15.8%, respectively.
34794805	4	55	theme	CNF/SCN	762:768	arg1	composites					770:779	the resultant CNF/SCN composites	748:779	the resultant CNF/SCN composites	748:779	Consequently, the resultant CNF/SCN composites exhibited remarkable in-plane thermal conductivity of 11.83 ± 0.41 W m-1 K-1 at 30 wt% SCN content, which was attributed to the significantly reduced interfacial phonon scattering.
34794805	0	56	theme	Cellulose/Carbon	74:89	arg1	nanocomposites					99:112	highly thermally conductive Cellulose/Carbon nitride nanocomposites	46:112	highly thermally conductive Cellulose/Carbon nitride nanocomposites	46:112	Self-assembled supramolecule for synthesizing highly thermally conductive Cellulose/Carbon nitride nanocomposites with improved flame retardancy.
34794805	5	57	from	retardancy	1033:1042	arg1	insulation					1012:1021	electrical insulation	1001:1021	electrical insulation	1001:1021	It also showed evident improvements in electrical insulation and flame retardancy compared with the pure CNF film, where the volume resistivity, peak heat release rate, and total heat release were remarkably enhanced by 1242% and reduced by 59.9% and 15.8%, respectively.
34794805	6	58	theme	materials	1335:1343	arg1	concentration					1311:1323	high concentration	1306:1323	high concentration of carbon materials	1306:1343	Further analysis of char residuals revealed a relatively dense surface, high concentration of carbon materials, and a high degree of graphitization, indicating that the char residual functioned as a robust physical barrier to effectively inhibit combustion.
34794805	6	58	theme	materials	1335:1343	arg1	degree					1357:1362	a high degree	1350:1362	a high degree of graphitization	1350:1380	Further analysis of char residuals revealed a relatively dense surface, high concentration of carbon materials, and a high degree of graphitization, indicating that the char residual functioned as a robust physical barrier to effectively inhibit combustion.
34794805	6	58	theme	materials	1335:1343	arg1	surface					1297:1303	a relatively dense surface	1278:1303	a relatively dense surface	1278:1303	Further analysis of char residuals revealed a relatively dense surface, high concentration of carbon materials, and a high degree of graphitization, indicating that the char residual functioned as a robust physical barrier to effectively inhibit combustion.
34794805	4	59	theme	30 wt	861:865	arg1	content					872:878	30 wt% SCN content	861:878	30 wt% SCN content	861:878	Consequently, the resultant CNF/SCN composites exhibited remarkable in-plane thermal conductivity of 11.83 ± 0.41 W m-1 K-1 at 30 wt% SCN content, which was attributed to the significantly reduced interfacial phonon scattering.
34794805	0	60	theme	conductive	63:72	arg1	nanocomposites					99:112	highly thermally conductive Cellulose/Carbon nitride nanocomposites	46:112	highly thermally conductive Cellulose/Carbon nitride nanocomposites	46:112	Self-assembled supramolecule for synthesizing highly thermally conductive Cellulose/Carbon nitride nanocomposites with improved flame retardancy.
34794805	6	61	theme	dense	1291:1295	arg1	surface					1297:1303	a relatively dense surface	1278:1303	a relatively dense surface	1278:1303	Further analysis of char residuals revealed a relatively dense surface, high concentration of carbon materials, and a high degree of graphitization, indicating that the char residual functioned as a robust physical barrier to effectively inhibit combustion.
34794805	7	62	theme	broader	1650:1656	arg1	applications					1658:1669	broader applications	1650:1669	broader applications of carbon nitride in thermal management	1650:1709	This study provides a facile approach to achieving high-efficiency improvements in thermal conductivity and flame retardancy, and simultaneously facilitating broader applications of carbon nitride in thermal management.
34794805	1	63	theme	excellent	189:197	arg1	conductivity					207:218	excellent thermal conductivity	189:218	excellent thermal conductivity	189:218	The fabrication of polymer composites with excellent thermal conductivity typically involves complex matrix or fillers modifications.
34794805	7	64	from	applications	1658:1669	arg1	management					1700:1709	thermal management	1692:1709	thermal management	1692:1709	This study provides a facile approach to achieving high-efficiency improvements in thermal conductivity and flame retardancy, and simultaneously facilitating broader applications of carbon nitride in thermal management.
34794805	2	65	theme	CNF	411:413	arg1	composites					463:472	highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites	362:472	highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites	362:472	This study proposed a simple technique based on precursor selection for obtaining highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites.
34794805	3	66	theme	scanning	664:671	arg1	microscopy					682:691	scanning electron microscopy	664:691	scanning electron microscopy	664:691	Fourier-transform infrared tests demonstrated the construction of hydrogen bonds between CNF and SCN; a highly ordered structure and relatively compact in-plane stacking were confirmed via scanning electron microscopy and X-ray diffraction characterizations.
34794805	4	67	theme	interfacial	931:941	arg1	scattering					950:959	the significantly reduced interfacial phonon scattering	905:959	the significantly reduced interfacial phonon scattering	905:959	Consequently, the resultant CNF/SCN composites exhibited remarkable in-plane thermal conductivity of 11.83 ± 0.41 W m-1 K-1 at 30 wt% SCN content, which was attributed to the significantly reduced interfacial phonon scattering.
34794805	5	68	from	improvements	985:996	arg1	insulation					1012:1021	electrical insulation	1001:1021	electrical insulation	1001:1021	It also showed evident improvements in electrical insulation and flame retardancy compared with the pure CNF film, where the volume resistivity, peak heat release rate, and total heat release were remarkably enhanced by 1242% and reduced by 59.9% and 15.8%, respectively.
34794805	1	69	theme	polymer	165:171	arg1	composites					173:182	polymer composites	165:182	polymer composites	165:182	The fabrication of polymer composites with excellent thermal conductivity typically involves complex matrix or fillers modifications.
34794805	3	70	theme	in-plane	627:634	arg1	stacking					636:643	relatively compact in-plane stacking	608:643	relatively compact in-plane stacking	608:643	Fourier-transform infrared tests demonstrated the construction of hydrogen bonds between CNF and SCN; a highly ordered structure and relatively compact in-plane stacking were confirmed via scanning electron microscopy and X-ray diffraction characterizations.
34794805	4	71	theme	reduced	923:929	arg1	scattering					950:959	the significantly reduced interfacial phonon scattering	905:959	the significantly reduced interfacial phonon scattering	905:959	Consequently, the resultant CNF/SCN composites exhibited remarkable in-plane thermal conductivity of 11.83 ± 0.41 W m-1 K-1 at 30 wt% SCN content, which was attributed to the significantly reduced interfacial phonon scattering.
34794805	1	72	theme	composites	173:182	arg1	fabrication					150:160	The fabrication	146:160	The fabrication of polymer composites with excellent thermal conductivity	146:218	The fabrication of polymer composites with excellent thermal conductivity typically involves complex matrix or fillers modifications.
34794805	4	73	theme	SCN	868:870	arg1	content					872:878	30 wt% SCN content	861:878	30 wt% SCN content	861:878	Consequently, the resultant CNF/SCN composites exhibited remarkable in-plane thermal conductivity of 11.83 ± 0.41 W m-1 K-1 at 30 wt% SCN content, which was attributed to the significantly reduced interfacial phonon scattering.
34794805	5	74	theme	release	1117:1123	arg1	rate					1125:1128	peak heat release rate	1107:1128	peak heat release rate	1107:1128	It also showed evident improvements in electrical insulation and flame retardancy compared with the pure CNF film, where the volume resistivity, peak heat release rate, and total heat release were remarkably enhanced by 1242% and reduced by 59.9% and 15.8%, respectively.
34794805	5	75	theme	total	1135:1139	arg1	release					1146:1152	total heat release	1135:1152	total heat release	1135:1152	It also showed evident improvements in electrical insulation and flame retardancy compared with the pure CNF film, where the volume resistivity, peak heat release rate, and total heat release were remarkably enhanced by 1242% and reduced by 59.9% and 15.8%, respectively.
34794805	5	76	theme	evident	977:983	arg1	improvements					985:996	evident improvements	977:996	evident improvements in electrical insulation	977:1021	It also showed evident improvements in electrical insulation and flame retardancy compared with the pure CNF film, where the volume resistivity, peak heat release rate, and total heat release were remarkably enhanced by 1242% and reduced by 59.9% and 15.8%, respectively.
34794805	0	77	theme	nitride	91:97	arg1	nanocomposites					99:112	highly thermally conductive Cellulose/Carbon nitride nanocomposites	46:112	highly thermally conductive Cellulose/Carbon nitride nanocomposites	46:112	Self-assembled supramolecule for synthesizing highly thermally conductive Cellulose/Carbon nitride nanocomposites with improved flame retardancy.
34794805	2	78	theme	simple	302:307	arg1	technique					309:317	a simple technique	300:317	a simple technique based on precursor selection for obtaining highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites	300:472	This study proposed a simple technique based on precursor selection for obtaining highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites.
34794805	2	79	theme	nitride	449:455	arg1	composites					463:472	highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites	362:472	highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites	362:472	This study proposed a simple technique based on precursor selection for obtaining highly thermally conductive cellulose nanofiber (CNF)/supramolecule-synthesized carbon nitride (SCN) composites.
32933688	0	0	theme	hemostatic	86:95	arg1	material					97:104	a bioactive hemostatic material	74:104	a bioactive hemostatic material	74:104	Preparation and characterization of tissue-factor-loaded alginate: Toward a bioactive hemostatic material.
32933688	2	1	with	combined	369:376	arg1	biopolymers					392:402	alginate biopolymers	383:402	alginate biopolymers	383:402	Inspired by TF's effectiveness in initiating coagulation, this work aims to develop hemostatic materials with TF-integrated liposomes, which combined with alginate biopolymers are designed as composite pastes or hydrogels cross-linked with Ca2+.
32933688	4	2	from	pastes	703:708	arg1	rate					679:682	The proteoliposome release rate	652:682	The proteoliposome release rate from the composite pastes	652:708	The proteoliposome release rate from the composite pastes increased with the decrease of alginate concentration from 3% to 1%, or relative to the corresponding hydrogels.
32933688	6	3	theme	TF	906:907	arg1	activity					922:929	TF procoagulant activity	906:929	TF procoagulant activity	906:929	The combination with alginate enhanced TF procoagulant activity, and most importantly the resultant composites exhibited superior hemostatic performance, yielding a shortest blood clotting time of 1.5 min while untreated blood took 14.2 min to clot, with no cytotoxicity against mammalian cells.
32933688	4	4	theme	1	775:775	arg1	%					770:770	%	770:770	%	770:770	The proteoliposome release rate from the composite pastes increased with the decrease of alginate concentration from 3% to 1%, or relative to the corresponding hydrogels.
32933688	3	5	theme	alginate	565:572	arg1	matrices					574:581	alginate matrices	565:581	alginate matrices	565:581	Fluorescence measurements revealed that the proteoliposomes were evenly distributed within alginate matrices, which also remained intact after release into simulated body fluid.
32933688	4	6	theme	alginate	741:748	arg1	concentration					750:762	alginate concentration	741:762	alginate concentration	741:762	The proteoliposome release rate from the composite pastes increased with the decrease of alginate concentration from 3% to 1%, or relative to the corresponding hydrogels.
32933688	6	7	theme	min	1068:1070	arg1	time					1056:1059	a shortest blood clotting time	1030:1059	a shortest blood clotting time of 1.5 min	1030:1070	The combination with alginate enhanced TF procoagulant activity, and most importantly the resultant composites exhibited superior hemostatic performance, yielding a shortest blood clotting time of 1.5 min while untreated blood took 14.2 min to clot, with no cytotoxicity against mammalian cells.
32933688	6	8	with	combination	871:881	arg1	alginate					888:895	alginate	888:895	alginate	888:895	The combination with alginate enhanced TF procoagulant activity, and most importantly the resultant composites exhibited superior hemostatic performance, yielding a shortest blood clotting time of 1.5 min while untreated blood took 14.2 min to clot, with no cytotoxicity against mammalian cells.
32933688	4	9	theme	3	769:769	arg1	%					770:770	%	770:770	%	770:770	The proteoliposome release rate from the composite pastes increased with the decrease of alginate concentration from 3% to 1%, or relative to the corresponding hydrogels.
32933688	4	10	theme	composite	693:701	arg1	pastes					703:708	the composite pastes	689:708	the composite pastes	689:708	The proteoliposome release rate from the composite pastes increased with the decrease of alginate concentration from 3% to 1%, or relative to the corresponding hydrogels.
32933688	6	11	theme	hemostatic	997:1006	arg1	performance					1008:1018	superior hemostatic performance	988:1018	superior hemostatic performance	988:1018	The combination with alginate enhanced TF procoagulant activity, and most importantly the resultant composites exhibited superior hemostatic performance, yielding a shortest blood clotting time of 1.5 min while untreated blood took 14.2 min to clot, with no cytotoxicity against mammalian cells.
32933688	1	12	theme	Tissue	107:112	arg1	protein					148:154	an integral membrane protein	127:154	an integral membrane protein	127:154	Tissue factor (TF), an integral membrane protein, is by far the most potent known triggering agent of blood coagulation.
32933688	1	12	theme	Tissue	107:112	arg1	TF					122:123	TF	122:123	TF	122:123	Tissue factor (TF), an integral membrane protein, is by far the most potent known triggering agent of blood coagulation.
32933688	1	12	theme	Tissue	107:112	arg1	factor					114:119	Tissue factor	107:119	Tissue factor (TF)	107:124	Tissue factor (TF), an integral membrane protein, is by far the most potent known triggering agent of blood coagulation.
32933688	1	12	theme	Tissue	107:112	arg1	agent					200:204	the most potent known triggering agent	167:204	the most potent known triggering agent of blood coagulation	167:225	Tissue factor (TF), an integral membrane protein, is by far the most potent known triggering agent of blood coagulation.
32933688	6	13	theme	untreated	1078:1086	arg1	blood					1088:1092	untreated blood	1078:1092	untreated blood	1078:1092	The combination with alginate enhanced TF procoagulant activity, and most importantly the resultant composites exhibited superior hemostatic performance, yielding a shortest blood clotting time of 1.5 min while untreated blood took 14.2 min to clot, with no cytotoxicity against mammalian cells.
32933688	2	14	theme	composite	420:428	arg1	pastes					430:435	composite pastes	420:435	composite pastes	420:435	Inspired by TF's effectiveness in initiating coagulation, this work aims to develop hemostatic materials with TF-integrated liposomes, which combined with alginate biopolymers are designed as composite pastes or hydrogels cross-linked with Ca2+.
32933688	2	14	theme	composite	420:428	arg1	liposomes					352:360	TF-integrated liposomes	338:360	TF-integrated liposomes	338:360	Inspired by TF's effectiveness in initiating coagulation, this work aims to develop hemostatic materials with TF-integrated liposomes, which combined with alginate biopolymers are designed as composite pastes or hydrogels cross-linked with Ca2+.
32933688	6	15	theme	superior	988:995	arg1	performance					1008:1018	superior hemostatic performance	988:1018	superior hemostatic performance	988:1018	The combination with alginate enhanced TF procoagulant activity, and most importantly the resultant composites exhibited superior hemostatic performance, yielding a shortest blood clotting time of 1.5 min while untreated blood took 14.2 min to clot, with no cytotoxicity against mammalian cells.
32933688	6	16	theme	shortest	1032:1039	arg1	time					1056:1059	a shortest blood clotting time	1030:1059	a shortest blood clotting time of 1.5 min	1030:1070	The combination with alginate enhanced TF procoagulant activity, and most importantly the resultant composites exhibited superior hemostatic performance, yielding a shortest blood clotting time of 1.5 min while untreated blood took 14.2 min to clot, with no cytotoxicity against mammalian cells.
32933688	6	17	theme	mammalian	1146:1154	arg1	cells					1156:1160	mammalian cells	1146:1160	mammalian cells	1146:1160	The combination with alginate enhanced TF procoagulant activity, and most importantly the resultant composites exhibited superior hemostatic performance, yielding a shortest blood clotting time of 1.5 min while untreated blood took 14.2 min to clot, with no cytotoxicity against mammalian cells.
32933688	1	18	theme	potent	176:181	arg1	agent					200:204	the most potent known triggering agent	167:204	the most potent known triggering agent of blood coagulation	167:225	Tissue factor (TF), an integral membrane protein, is by far the most potent known triggering agent of blood coagulation.
32933688	1	18	theme	potent	176:181	arg1	factor					114:119	Tissue factor	107:119	Tissue factor (TF)	107:124	Tissue factor (TF), an integral membrane protein, is by far the most potent known triggering agent of blood coagulation.
32933688	0	19	theme	alginate	57:64	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of tissue-factor-loaded alginate: Toward a bioactive hemostatic material.
32933688	0	19	theme	alginate	57:64	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of tissue-factor-loaded alginate: Toward a bioactive hemostatic material.
32933688	4	20	theme	release	671:677	arg1	rate					679:682	The proteoliposome release rate	652:682	The proteoliposome release rate from the composite pastes	652:708	The proteoliposome release rate from the composite pastes increased with the decrease of alginate concentration from 3% to 1%, or relative to the corresponding hydrogels.
32933688	4	21	theme	proteoliposome	656:669	arg1	rate					679:682	The proteoliposome release rate	652:682	The proteoliposome release rate from the composite pastes	652:708	The proteoliposome release rate from the composite pastes increased with the decrease of alginate concentration from 3% to 1%, or relative to the corresponding hydrogels.
32933688	1	22	theme	known	183:187	arg1	agent					200:204	the most potent known triggering agent	167:204	the most potent known triggering agent of blood coagulation	167:225	Tissue factor (TF), an integral membrane protein, is by far the most potent known triggering agent of blood coagulation.
32933688	1	22	theme	known	183:187	arg1	factor					114:119	Tissue factor	107:119	Tissue factor (TF)	107:124	Tissue factor (TF), an integral membrane protein, is by far the most potent known triggering agent of blood coagulation.
32933688	0	23	theme	tissue-factor-loaded	36:55	arg1	alginate					57:64	tissue-factor-loaded alginate	36:64	tissue-factor-loaded alginate	36:64	Preparation and characterization of tissue-factor-loaded alginate: Toward a bioactive hemostatic material.
32933688	6	24	theme	resultant	957:965	arg1	composites					967:976	the resultant composites	953:976	the resultant composites	953:976	The combination with alginate enhanced TF procoagulant activity, and most importantly the resultant composites exhibited superior hemostatic performance, yielding a shortest blood clotting time of 1.5 min while untreated blood took 14.2 min to clot, with no cytotoxicity against mammalian cells.
32933688	6	25	theme	blood	1041:1045	arg1	time					1056:1059	a shortest blood clotting time	1030:1059	a shortest blood clotting time of 1.5 min	1030:1070	The combination with alginate enhanced TF procoagulant activity, and most importantly the resultant composites exhibited superior hemostatic performance, yielding a shortest blood clotting time of 1.5 min while untreated blood took 14.2 min to clot, with no cytotoxicity against mammalian cells.
32933688	5	26	theme	swelling	848:855	arg1	property					857:864	a swelling property	846:864	a swelling property	846:864	The latter also showed a swelling property.
32933688	1	27	theme	triggering	189:198	arg1	agent					200:204	the most potent known triggering agent	167:204	the most potent known triggering agent of blood coagulation	167:225	Tissue factor (TF), an integral membrane protein, is by far the most potent known triggering agent of blood coagulation.
32933688	1	27	theme	triggering	189:198	arg1	factor					114:119	Tissue factor	107:119	Tissue factor (TF)	107:124	Tissue factor (TF), an integral membrane protein, is by far the most potent known triggering agent of blood coagulation.
32933688	2	28	theme	hemostatic	312:321	arg1	materials					323:331	hemostatic materials	312:331	hemostatic materials with TF-integrated liposomes, which combined with alginate biopolymers are designed as composite pastes or hydrogels cross-linked with Ca2+	312:471	Inspired by TF's effectiveness in initiating coagulation, this work aims to develop hemostatic materials with TF-integrated liposomes, which combined with alginate biopolymers are designed as composite pastes or hydrogels cross-linked with Ca2+.
32933688	6	29	theme	procoagulant	909:920	arg1	activity					922:929	TF procoagulant activity	906:929	TF procoagulant activity	906:929	The combination with alginate enhanced TF procoagulant activity, and most importantly the resultant composites exhibited superior hemostatic performance, yielding a shortest blood clotting time of 1.5 min while untreated blood took 14.2 min to clot, with no cytotoxicity against mammalian cells.
32933688	1	30	theme	membrane	139:146	arg1	factor					114:119	Tissue factor	107:119	Tissue factor (TF)	107:124	Tissue factor (TF), an integral membrane protein, is by far the most potent known triggering agent of blood coagulation.
32933688	1	30	theme	membrane	139:146	arg1	protein					148:154	an integral membrane protein	127:154	an integral membrane protein	127:154	Tissue factor (TF), an integral membrane protein, is by far the most potent known triggering agent of blood coagulation.
32933688	4	31	dep	%	770:770	arg1	to					772:773	to	772:773	to	772:773	The proteoliposome release rate from the composite pastes increased with the decrease of alginate concentration from 3% to 1%, or relative to the corresponding hydrogels.
32933688	2	32	with	materials	323:331	arg1	pastes					430:435	composite pastes	420:435	composite pastes	420:435	Inspired by TF's effectiveness in initiating coagulation, this work aims to develop hemostatic materials with TF-integrated liposomes, which combined with alginate biopolymers are designed as composite pastes or hydrogels cross-linked with Ca2+.
32933688	2	32	with	materials	323:331	arg1	hydrogels					440:448	hydrogels	440:448	hydrogels	440:448	Inspired by TF's effectiveness in initiating coagulation, this work aims to develop hemostatic materials with TF-integrated liposomes, which combined with alginate biopolymers are designed as composite pastes or hydrogels cross-linked with Ca2+.
32933688	2	32	with	materials	323:331	arg1	liposomes					352:360	TF-integrated liposomes	338:360	TF-integrated liposomes	338:360	Inspired by TF's effectiveness in initiating coagulation, this work aims to develop hemostatic materials with TF-integrated liposomes, which combined with alginate biopolymers are designed as composite pastes or hydrogels cross-linked with Ca2+.
32933688	3	33	theme	body	640:643	arg1	fluid					645:649	simulated body fluid	630:649	simulated body fluid	630:649	Fluorescence measurements revealed that the proteoliposomes were evenly distributed within alginate matrices, which also remained intact after release into simulated body fluid.
32933688	4	34	theme	concentration	750:762	arg1	decrease					729:736	the decrease	725:736	the decrease of alginate concentration	725:762	The proteoliposome release rate from the composite pastes increased with the decrease of alginate concentration from 3% to 1%, or relative to the corresponding hydrogels.
32933688	3	35	theme	Fluorescence	474:485	arg1	measurements					487:498	Fluorescence measurements	474:498	Fluorescence measurements	474:498	Fluorescence measurements revealed that the proteoliposomes were evenly distributed within alginate matrices, which also remained intact after release into simulated body fluid.
32933688	6	36	theme	clotting	1047:1054	arg1	time					1056:1059	a shortest blood clotting time	1030:1059	a shortest blood clotting time of 1.5 min	1030:1070	The combination with alginate enhanced TF procoagulant activity, and most importantly the resultant composites exhibited superior hemostatic performance, yielding a shortest blood clotting time of 1.5 min while untreated blood took 14.2 min to clot, with no cytotoxicity against mammalian cells.
32933688	0	37	theme	bioactive	76:84	arg1	material					97:104	a bioactive hemostatic material	74:104	a bioactive hemostatic material	74:104	Preparation and characterization of tissue-factor-loaded alginate: Toward a bioactive hemostatic material.
32933688	2	38	theme	alginate	383:390	arg1	biopolymers					392:402	alginate biopolymers	383:402	alginate biopolymers	383:402	Inspired by TF's effectiveness in initiating coagulation, this work aims to develop hemostatic materials with TF-integrated liposomes, which combined with alginate biopolymers are designed as composite pastes or hydrogels cross-linked with Ca2+.
32933688	4	39	theme	corresponding	798:810	arg1	hydrogels					812:820	the corresponding hydrogels	794:820	the corresponding hydrogels	794:820	The proteoliposome release rate from the composite pastes increased with the decrease of alginate concentration from 3% to 1%, or relative to the corresponding hydrogels.
32933688	1	40	theme	integral	130:137	arg1	factor					114:119	Tissue factor	107:119	Tissue factor (TF)	107:124	Tissue factor (TF), an integral membrane protein, is by far the most potent known triggering agent of blood coagulation.
32933688	1	40	theme	integral	130:137	arg1	protein					148:154	an integral membrane protein	127:154	an integral membrane protein	127:154	Tissue factor (TF), an integral membrane protein, is by far the most potent known triggering agent of blood coagulation.
32933688	1	41	theme	blood	209:213	arg1	coagulation					215:225	blood coagulation	209:225	blood coagulation	209:225	Tissue factor (TF), an integral membrane protein, is by far the most potent known triggering agent of blood coagulation.
32933688	3	42	theme	simulated	630:638	arg1	fluid					645:649	simulated body fluid	630:649	simulated body fluid	630:649	Fluorescence measurements revealed that the proteoliposomes were evenly distributed within alginate matrices, which also remained intact after release into simulated body fluid.
32933688	2	43	theme	TF-integrated	338:350	arg1	pastes					430:435	composite pastes	420:435	composite pastes	420:435	Inspired by TF's effectiveness in initiating coagulation, this work aims to develop hemostatic materials with TF-integrated liposomes, which combined with alginate biopolymers are designed as composite pastes or hydrogels cross-linked with Ca2+.
32933688	2	43	theme	TF-integrated	338:350	arg1	hydrogels					440:448	hydrogels	440:448	hydrogels	440:448	Inspired by TF's effectiveness in initiating coagulation, this work aims to develop hemostatic materials with TF-integrated liposomes, which combined with alginate biopolymers are designed as composite pastes or hydrogels cross-linked with Ca2+.
32933688	2	43	theme	TF-integrated	338:350	arg1	liposomes					352:360	TF-integrated liposomes	338:360	TF-integrated liposomes	338:360	Inspired by TF's effectiveness in initiating coagulation, this work aims to develop hemostatic materials with TF-integrated liposomes, which combined with alginate biopolymers are designed as composite pastes or hydrogels cross-linked with Ca2+.
32933688	1	44	theme	coagulation	215:225	arg1	agent					200:204	the most potent known triggering agent	167:204	the most potent known triggering agent of blood coagulation	167:225	Tissue factor (TF), an integral membrane protein, is by far the most potent known triggering agent of blood coagulation.
32933688	1	44	theme	coagulation	215:225	arg1	factor					114:119	Tissue factor	107:119	Tissue factor (TF)	107:124	Tissue factor (TF), an integral membrane protein, is by far the most potent known triggering agent of blood coagulation.
33485889	1	0	theme	film	144:147	arg1	application					117:127	the application	113:127	the application of xylan based film in food and drug packaging	113:174	To realize the application of xylan based film in food and drug packaging, the poor mechanical property and film-forming property of xylan based film must be overcome.
33485889	2	1	theme	graphene	418:425	arg1	oxide					427:431	graphene oxide	418:431	graphene oxide (GO)	418:436	Herein, a good oxygen barrier composite film with desired mechanical properties was prepared based on carboxymethly xylan (CMX), chitosan (CS), and graphene oxide (GO).
33485889	7	2	from	promise	1139:1145	arg1	field					1154:1158	the field	1150:1158	the field of food packaging and wound dressing due to their excellent mechanical, oxygen barrier and antibacterial properties	1150:1274	Therefore, these films show great promise in the field of food packaging and wound dressing due to their excellent mechanical, oxygen barrier and antibacterial properties.
33485889	5	3	theme	graphene	966:973	arg1	oxide					975:979	graphene oxide	966:979	graphene oxide	966:979	The oxygen barrier properties of the composite films significantly increased with the addition of graphene oxide due to the dense, stacked multilayer structure.
33485889	1	4	theme	xylan	235:239	arg1	film					247:250	xylan based film	235:250	xylan based film	235:250	To realize the application of xylan based film in food and drug packaging, the poor mechanical property and film-forming property of xylan based film must be overcome.
33485889	5	5	theme	multilayer	1007:1016	arg1	structure					1018:1026	the dense, stacked multilayer structure	988:1026	the dense, stacked multilayer structure	988:1026	The oxygen barrier properties of the composite films significantly increased with the addition of graphene oxide due to the dense, stacked multilayer structure.
33485889	4	6	theme	Young	775:779	arg1	modulus					783:789	Young's modulus	775:789	Young's modulus of the composite film	775:811	As expected, the composite film with the 0.5% mass fraction of GO exhibited best mechanical property, among which the tensile stress, tensile strain, and Young's modulus of the composite film reached 50.81 MPa, 47.61%, and 1.39 GPa, respectively.
33485889	3	7	theme	electron	463:470	arg1	microscope					472:481	scanning electron microscope	454:481	scanning electron microscope	454:481	The results of scanning electron microscope revealed the composite film had a dense and continuous structure, which will endow the composite film with excellent mechanical property.
33485889	1	8	theme	based	241:245	arg1	film					247:250	xylan based film	235:250	xylan based film	235:250	To realize the application of xylan based film in food and drug packaging, the poor mechanical property and film-forming property of xylan based film must be overcome.
33485889	0	9	theme	packaging	79:87	arg1	application					89:99	packaging application	79:99	packaging application	79:99	Fabrication of flexible composite film based on xylan from pulping process for packaging application.
33485889	5	10	theme	oxide	975:979	arg1	addition					954:961	the addition	950:961	the addition of graphene oxide due to the dense, stacked multilayer structure	950:1026	The oxygen barrier properties of the composite films significantly increased with the addition of graphene oxide due to the dense, stacked multilayer structure.
33485889	3	11	theme	microscope	472:481	arg1	results					443:449	The results	439:449	The results of scanning electron microscope	439:481	The results of scanning electron microscope revealed the composite film had a dense and continuous structure, which will endow the composite film with excellent mechanical property.
33485889	2	12	theme	desired	320:326	arg1	properties					339:348	desired mechanical properties	320:348	desired mechanical properties	320:348	Herein, a good oxygen barrier composite film with desired mechanical properties was prepared based on carboxymethly xylan (CMX), chitosan (CS), and graphene oxide (GO).
33485889	3	13	with	film	580:583	arg1	property					611:618	excellent mechanical property	590:618	excellent mechanical property	590:618	The results of scanning electron microscope revealed the composite film had a dense and continuous structure, which will endow the composite film with excellent mechanical property.
33485889	1	14	theme	film	247:250	arg1	property					197:204	poor mechanical property	181:204	poor mechanical property	181:204	To realize the application of xylan based film in food and drug packaging, the poor mechanical property and film-forming property of xylan based film must be overcome.
33485889	1	14	theme	film	247:250	arg1	property					223:230	film-forming property	210:230	film-forming property	210:230	To realize the application of xylan based film in food and drug packaging, the poor mechanical property and film-forming property of xylan based film must be overcome.
33485889	7	15	theme	great	1133:1137	arg1	promise					1139:1145	great promise	1133:1145	great promise in the field of food packaging and wound dressing due to their excellent mechanical, oxygen barrier and antibacterial properties	1133:1274	Therefore, these films show great promise in the field of food packaging and wound dressing due to their excellent mechanical, oxygen barrier and antibacterial properties.
33485889	7	16	theme	wound	1182:1186	arg1	dressing					1188:1195	wound dressing	1182:1195	wound dressing	1182:1195	Therefore, these films show great promise in the field of food packaging and wound dressing due to their excellent mechanical, oxygen barrier and antibacterial properties.
33485889	4	17	theme	GO	684:685	arg1	fraction					672:679	the 0.5% mass fraction	658:679	the 0.5% mass fraction of GO	658:685	As expected, the composite film with the 0.5% mass fraction of GO exhibited best mechanical property, among which the tensile stress, tensile strain, and Young's modulus of the composite film reached 50.81 MPa, 47.61%, and 1.39 GPa, respectively.
33485889	7	18	theme	excellent	1210:1218	arg1	barrier					1239:1245	oxygen barrier and antibacterial properties	1232:1274	barrier	1239:1245	Therefore, these films show great promise in the field of food packaging and wound dressing due to their excellent mechanical, oxygen barrier and antibacterial properties.
33485889	4	19	theme	tensile	739:745	arg1	stress					747:752	the tensile stress	735:752	the tensile stress	735:752	As expected, the composite film with the 0.5% mass fraction of GO exhibited best mechanical property, among which the tensile stress, tensile strain, and Young's modulus of the composite film reached 50.81 MPa, 47.61%, and 1.39 GPa, respectively.
33485889	1	20	from	application	117:127	arg1	drug					161:164	drug	161:164	drug	161:164	To realize the application of xylan based film in food and drug packaging, the poor mechanical property and film-forming property of xylan based film must be overcome.
33485889	1	20	from	application	117:127	arg1	food					152:155	food	152:155	food	152:155	To realize the application of xylan based film in food and drug packaging, the poor mechanical property and film-forming property of xylan based film must be overcome.
33485889	3	21	theme	scanning	454:461	arg1	microscope					472:481	scanning electron microscope	454:481	scanning electron microscope	454:481	The results of scanning electron microscope revealed the composite film had a dense and continuous structure, which will endow the composite film with excellent mechanical property.
33485889	6	22	theme	composite	1048:1056	arg1	films					1058:1062	these composite films	1042:1062	these composite films	1042:1062	In addition, these composite films exhibited good antibacterial properties.
33485889	4	23	theme	tensile	755:761	arg1	strain					763:768	tensile strain	755:768	tensile strain	755:768	As expected, the composite film with the 0.5% mass fraction of GO exhibited best mechanical property, among which the tensile stress, tensile strain, and Young's modulus of the composite film reached 50.81 MPa, 47.61%, and 1.39 GPa, respectively.
33485889	2	24	theme	good	280:283	arg1	film					310:313	a good oxygen barrier composite film	278:313	a good oxygen barrier composite film with desired mechanical properties	278:348	Herein, a good oxygen barrier composite film with desired mechanical properties was prepared based on carboxymethly xylan (CMX), chitosan (CS), and graphene oxide (GO).
33485889	7	25	theme	dressing	1188:1195	arg1	field					1154:1158	the field	1150:1158	the field of food packaging and wound dressing due to their excellent mechanical, oxygen barrier and antibacterial properties	1150:1274	Therefore, these films show great promise in the field of food packaging and wound dressing due to their excellent mechanical, oxygen barrier and antibacterial properties.
33485889	6	26	theme	good	1074:1077	arg1	properties					1093:1102	good antibacterial properties	1074:1102	good antibacterial properties	1074:1102	In addition, these composite films exhibited good antibacterial properties.
33485889	4	27	theme	composite	638:646	arg1	film					648:651	the composite film	634:651	the composite film with the 0.5% mass fraction of GO	634:685	As expected, the composite film with the 0.5% mass fraction of GO exhibited best mechanical property, among which the tensile stress, tensile strain, and Young's modulus of the composite film reached 50.81 MPa, 47.61%, and 1.39 GPa, respectively.
33485889	5	28	theme	films	915:919	arg1	properties					887:896	The oxygen barrier properties	868:896	The oxygen barrier properties of the composite films	868:919	The oxygen barrier properties of the composite films significantly increased with the addition of graphene oxide due to the dense, stacked multilayer structure.
33485889	7	29	theme	food	1163:1166	arg1	packaging					1168:1176	food packaging	1163:1176	food packaging	1163:1176	Therefore, these films show great promise in the field of food packaging and wound dressing due to their excellent mechanical, oxygen barrier and antibacterial properties.
33485889	4	30	dep	exhibited	687:695	arg1	reached					813:819	reached	813:819	reached	813:819	As expected, the composite film with the 0.5% mass fraction of GO exhibited best mechanical property, among which the tensile stress, tensile strain, and Young's modulus of the composite film reached 50.81 MPa, 47.61%, and 1.39 GPa, respectively.
33485889	0	31	theme	composite	24:32	arg1	film					34:37	flexible composite film	15:37	flexible composite film	15:37	Fabrication of flexible composite film based on xylan from pulping process for packaging application.
33485889	5	32	theme	oxygen	872:877	arg1	properties					887:896	The oxygen barrier properties	868:896	The oxygen barrier properties of the composite films	868:919	The oxygen barrier properties of the composite films significantly increased with the addition of graphene oxide due to the dense, stacked multilayer structure.
33485889	4	33	theme	film	808:811	arg1	stress					747:752	the tensile stress	735:752	the tensile stress	735:752	As expected, the composite film with the 0.5% mass fraction of GO exhibited best mechanical property, among which the tensile stress, tensile strain, and Young's modulus of the composite film reached 50.81 MPa, 47.61%, and 1.39 GPa, respectively.
33485889	4	33	theme	film	808:811	arg1	strain					763:768	tensile strain	755:768	tensile strain	755:768	As expected, the composite film with the 0.5% mass fraction of GO exhibited best mechanical property, among which the tensile stress, tensile strain, and Young's modulus of the composite film reached 50.81 MPa, 47.61%, and 1.39 GPa, respectively.
33485889	4	33	theme	film	808:811	arg1	modulus					783:789	Young's modulus	775:789	Young's modulus of the composite film	775:811	As expected, the composite film with the 0.5% mass fraction of GO exhibited best mechanical property, among which the tensile stress, tensile strain, and Young's modulus of the composite film reached 50.81 MPa, 47.61%, and 1.39 GPa, respectively.
33485889	7	34	theme	mechanical	1220:1229	arg1	barrier					1239:1245	oxygen barrier and antibacterial properties	1232:1274	barrier	1239:1245	Therefore, these films show great promise in the field of food packaging and wound dressing due to their excellent mechanical, oxygen barrier and antibacterial properties.
33485889	4	35	theme	%	665:665	arg1	fraction					672:679	the 0.5% mass fraction	658:679	the 0.5% mass fraction of GO	658:685	As expected, the composite film with the 0.5% mass fraction of GO exhibited best mechanical property, among which the tensile stress, tensile strain, and Young's modulus of the composite film reached 50.81 MPa, 47.61%, and 1.39 GPa, respectively.
33485889	7	36	theme	antibacterial	1251:1263	arg1	properties					1265:1274	oxygen barrier and antibacterial properties	1232:1274	properties	1265:1274	Therefore, these films show great promise in the field of food packaging and wound dressing due to their excellent mechanical, oxygen barrier and antibacterial properties.
33485889	0	37	theme	flexible	15:22	arg1	film					34:37	flexible composite film	15:37	flexible composite film	15:37	Fabrication of flexible composite film based on xylan from pulping process for packaging application.
33485889	5	38	dep	dense	992:996	arg1	stacked					999:1005	stacked	999:1005	stacked	999:1005	The oxygen barrier properties of the composite films significantly increased with the addition of graphene oxide due to the dense, stacked multilayer structure.
33485889	4	39	theme	composite	798:806	arg1	film					808:811	the composite film	794:811	the composite film	794:811	As expected, the composite film with the 0.5% mass fraction of GO exhibited best mechanical property, among which the tensile stress, tensile strain, and Young's modulus of the composite film reached 50.81 MPa, 47.61%, and 1.39 GPa, respectively.
33485889	4	40	theme	0.5	662:664	arg1	%					665:665	%	665:665	%	665:665	As expected, the composite film with the 0.5% mass fraction of GO exhibited best mechanical property, among which the tensile stress, tensile strain, and Young's modulus of the composite film reached 50.81 MPa, 47.61%, and 1.39 GPa, respectively.
33485889	7	41	theme	oxygen	1232:1237	arg1	barrier					1239:1245	oxygen barrier and antibacterial properties	1232:1274	barrier	1239:1245	Therefore, these films show great promise in the field of food packaging and wound dressing due to their excellent mechanical, oxygen barrier and antibacterial properties.
33485889	7	42	theme	packaging	1168:1176	arg1	field					1154:1158	the field	1150:1158	the field of food packaging and wound dressing due to their excellent mechanical, oxygen barrier and antibacterial properties	1150:1274	Therefore, these films show great promise in the field of food packaging and wound dressing due to their excellent mechanical, oxygen barrier and antibacterial properties.
33485889	5	43	theme	barrier	879:885	arg1	properties					887:896	The oxygen barrier properties	868:896	The oxygen barrier properties of the composite films	868:919	The oxygen barrier properties of the composite films significantly increased with the addition of graphene oxide due to the dense, stacked multilayer structure.
33485889	3	44	theme	composite	570:578	arg1	film					580:583	the composite film	566:583	the composite film with excellent mechanical property	566:618	The results of scanning electron microscope revealed the composite film had a dense and continuous structure, which will endow the composite film with excellent mechanical property.
33485889	3	45	theme	dense	517:521	arg1	structure					538:546	a dense and continuous structure	515:546	a dense and continuous structure	515:546	The results of scanning electron microscope revealed the composite film had a dense and continuous structure, which will endow the composite film with excellent mechanical property.
33485889	1	46	theme	poor	181:184	arg1	property					197:204	poor mechanical property	181:204	poor mechanical property	181:204	To realize the application of xylan based film in food and drug packaging, the poor mechanical property and film-forming property of xylan based film must be overcome.
33485889	0	47	theme	film	34:37	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of flexible composite film	0:37	Fabrication of flexible composite film based on xylan from pulping process for packaging application.
33485889	1	48	dep	property	197:204	arg1	the					177:179	the	177:179	the	177:179	To realize the application of xylan based film in food and drug packaging, the poor mechanical property and film-forming property of xylan based film must be overcome.
33485889	1	49	theme	mechanical	186:195	arg1	property					197:204	poor mechanical property	181:204	poor mechanical property	181:204	To realize the application of xylan based film in food and drug packaging, the poor mechanical property and film-forming property of xylan based film must be overcome.
33485889	2	50	dep	oxide	427:431	arg1	GO					434:435	GO	434:435	GO	434:435	Herein, a good oxygen barrier composite film with desired mechanical properties was prepared based on carboxymethly xylan (CMX), chitosan (CS), and graphene oxide (GO).
33485889	4	51	theme	mass	667:670	arg1	fraction					672:679	the 0.5% mass fraction	658:679	the 0.5% mass fraction of GO	658:685	As expected, the composite film with the 0.5% mass fraction of GO exhibited best mechanical property, among which the tensile stress, tensile strain, and Young's modulus of the composite film reached 50.81 MPa, 47.61%, and 1.39 GPa, respectively.
33485889	1	52	dep	food	152:155	arg1	packaging					166:174	packaging	166:174	packaging	166:174	To realize the application of xylan based film in food and drug packaging, the poor mechanical property and film-forming property of xylan based film must be overcome.
33485889	2	53	theme	mechanical	328:337	arg1	properties					339:348	desired mechanical properties	320:348	desired mechanical properties	320:348	Herein, a good oxygen barrier composite film with desired mechanical properties was prepared based on carboxymethly xylan (CMX), chitosan (CS), and graphene oxide (GO).
33485889	2	54	with	film	310:313	arg1	properties					339:348	desired mechanical properties	320:348	desired mechanical properties	320:348	Herein, a good oxygen barrier composite film with desired mechanical properties was prepared based on carboxymethly xylan (CMX), chitosan (CS), and graphene oxide (GO).
33485889	5	55	theme	dense	992:996	arg1	structure					1018:1026	the dense, stacked multilayer structure	988:1026	the dense, stacked multilayer structure	988:1026	The oxygen barrier properties of the composite films significantly increased with the addition of graphene oxide due to the dense, stacked multilayer structure.
33485889	3	56	theme	excellent	590:598	arg1	property					611:618	excellent mechanical property	590:618	excellent mechanical property	590:618	The results of scanning electron microscope revealed the composite film had a dense and continuous structure, which will endow the composite film with excellent mechanical property.
33485889	5	57	theme	composite	905:913	arg1	films					915:919	the composite films	901:919	the composite films	901:919	The oxygen barrier properties of the composite films significantly increased with the addition of graphene oxide due to the dense, stacked multilayer structure.
33485889	2	58	theme	composite	300:308	arg1	film					310:313	a good oxygen barrier composite film	278:313	a good oxygen barrier composite film with desired mechanical properties	278:348	Herein, a good oxygen barrier composite film with desired mechanical properties was prepared based on carboxymethly xylan (CMX), chitosan (CS), and graphene oxide (GO).
33485889	3	59	theme	composite	496:504	arg1	film					506:509	the composite film	492:509	the composite film had a dense and continuous structure, which will endow the composite film with excellent mechanical property	492:618	The results of scanning electron microscope revealed the composite film had a dense and continuous structure, which will endow the composite film with excellent mechanical property.
33485889	3	60	theme	mechanical	600:609	arg1	property					611:618	excellent mechanical property	590:618	excellent mechanical property	590:618	The results of scanning electron microscope revealed the composite film had a dense and continuous structure, which will endow the composite film with excellent mechanical property.
33485889	4	61	theme	best	697:700	arg1	property					713:720	best mechanical property	697:720	best mechanical property	697:720	As expected, the composite film with the 0.5% mass fraction of GO exhibited best mechanical property, among which the tensile stress, tensile strain, and Young's modulus of the composite film reached 50.81 MPa, 47.61%, and 1.39 GPa, respectively.
33485889	2	62	theme	barrier	292:298	arg1	film					310:313	a good oxygen barrier composite film	278:313	a good oxygen barrier composite film with desired mechanical properties	278:348	Herein, a good oxygen barrier composite film with desired mechanical properties was prepared based on carboxymethly xylan (CMX), chitosan (CS), and graphene oxide (GO).
33485889	6	63	theme	antibacterial	1079:1091	arg1	properties					1093:1102	good antibacterial properties	1074:1102	good antibacterial properties	1074:1102	In addition, these composite films exhibited good antibacterial properties.
33485889	2	64	theme	oxygen	285:290	arg1	film					310:313	a good oxygen barrier composite film	278:313	a good oxygen barrier composite film with desired mechanical properties	278:348	Herein, a good oxygen barrier composite film with desired mechanical properties was prepared based on carboxymethly xylan (CMX), chitosan (CS), and graphene oxide (GO).
33485889	2	65	theme	carboxymethly	372:384	arg1	xylan					386:390	carboxymethly xylan	372:390	carboxymethly xylan (CMX)	372:396	Herein, a good oxygen barrier composite film with desired mechanical properties was prepared based on carboxymethly xylan (CMX), chitosan (CS), and graphene oxide (GO).
33485889	2	65	theme	carboxymethly	372:384	arg1	CMX					393:395	CMX	393:395	CMX	393:395	Herein, a good oxygen barrier composite film with desired mechanical properties was prepared based on carboxymethly xylan (CMX), chitosan (CS), and graphene oxide (GO).
33485889	3	66	theme	continuous	527:536	arg1	structure					538:546	a dense and continuous structure	515:546	a dense and continuous structure	515:546	The results of scanning electron microscope revealed the composite film had a dense and continuous structure, which will endow the composite film with excellent mechanical property.
33485889	1	67	theme	xylan	132:136	arg1	film					144:147	xylan based film	132:147	xylan based film	132:147	To realize the application of xylan based film in food and drug packaging, the poor mechanical property and film-forming property of xylan based film must be overcome.
33485889	1	68	theme	film-forming	210:221	arg1	property					223:230	film-forming property	210:230	film-forming property	210:230	To realize the application of xylan based film in food and drug packaging, the poor mechanical property and film-forming property of xylan based film must be overcome.
33485889	4	69	with	film	648:651	arg1	fraction					672:679	the 0.5% mass fraction	658:679	the 0.5% mass fraction of GO	658:685	As expected, the composite film with the 0.5% mass fraction of GO exhibited best mechanical property, among which the tensile stress, tensile strain, and Young's modulus of the composite film reached 50.81 MPa, 47.61%, and 1.39 GPa, respectively.
33485889	4	70	theme	mechanical	702:711	arg1	property					713:720	best mechanical property	697:720	best mechanical property	697:720	As expected, the composite film with the 0.5% mass fraction of GO exhibited best mechanical property, among which the tensile stress, tensile strain, and Young's modulus of the composite film reached 50.81 MPa, 47.61%, and 1.39 GPa, respectively.
33485889	1	71	theme	based	138:142	arg1	film					144:147	xylan based film	132:147	xylan based film	132:147	To realize the application of xylan based film in food and drug packaging, the poor mechanical property and film-forming property of xylan based film must be overcome.
34606920	0	0	theme	xylooligosaccharides	80:99	arg1	production					101:110	pectin and xylooligosaccharides production	69:110	pectin and xylooligosaccharides production from cocoa pod husks	69:131	Citric acid assisted hydrothermal pretreatment for the extraction of pectin and xylooligosaccharides production from cocoa pod husks.
34606920	2	1	theme	fraction	465:472	arg1	hydrolysis					441:450	efficient enzymatic hydrolysis	421:450	efficient enzymatic hydrolysis of the solid fraction	421:472	CPH́s pectin recovery was improved with concomitant production of xylooligosaccharides (XOS) through efficient enzymatic hydrolysis of the solid fraction.
34606920	7	2	theme	biomolecules	1073:1084	arg1	production					1047:1056	the production	1043:1056	the production of value-added biomolecules under a biorefinery concept	1043:1112	Great perspectives were observed in the implementation of CPH hydrothermal pretreatment for the production of value-added biomolecules under a biorefinery concept.
34606920	2	3	theme	CPH́s	320:324	arg1	recovery					333:340	CPH́s pectin recovery	320:340	CPH́s pectin recovery	320:340	CPH́s pectin recovery was improved with concomitant production of xylooligosaccharides (XOS) through efficient enzymatic hydrolysis of the solid fraction.
34606920	6	4	theme	cellulosic	858:867	arg1	fraction					869:876	the cellulosic fraction	854:876	the cellulosic fraction	854:876	The enzymatic conversion efficiency of the cellulosic fraction was 58.9%, leading to a production of 92.4 kg of glucose per ton of CPH.
34606920	1	5	theme	cocoa	243:247	arg1	CPH					260:262	CPH	260:262	CPH	260:262	The main purpose of this work was the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery.
34606920	1	5	theme	cocoa	243:247	arg1	husks					253:257	cocoa pod husks	243:257	cocoa pod husks (CPH)	243:263	The main purpose of this work was the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery.
34606920	2	6	theme	solid	459:463	arg1	fraction					465:472	the solid fraction	455:472	the solid fraction	455:472	CPH́s pectin recovery was improved with concomitant production of xylooligosaccharides (XOS) through efficient enzymatic hydrolysis of the solid fraction.
34606920	1	7	theme	pectin	303:308	arg1	recovery					310:317	pectin recovery	303:317	pectin recovery	303:317	The main purpose of this work was the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery.
34606920	1	8	theme	work	159:162	arg1	development					172:182	the development	168:182	the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery	168:317	The main purpose of this work was the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery.
34606920	1	8	theme	work	159:162	arg1	purpose					143:149	The main purpose	134:149	The main purpose of this work	134:162	The main purpose of this work was the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery.
34606920	1	9	theme	pod	249:251	arg1	CPH					260:262	CPH	260:262	CPH	260:262	The main purpose of this work was the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery.
34606920	1	9	theme	pod	249:251	arg1	husks					253:257	cocoa pod husks	243:257	cocoa pod husks (CPH)	243:263	The main purpose of this work was the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery.
34606920	6	10	theme	fraction	869:876	arg1	efficiency					840:849	The enzymatic conversion efficiency	815:849	The enzymatic conversion efficiency of the cellulosic fraction	815:876	The enzymatic conversion efficiency of the cellulosic fraction was 58.9%, leading to a production of 92.4 kg of glucose per ton of CPH.
34606920	6	10	theme	fraction	869:876	arg1	%					886:886	58.9%	882:886	58.9%	882:886	The enzymatic conversion efficiency of the cellulosic fraction was 58.9%, leading to a production of 92.4 kg of glucose per ton of CPH.
34606920	1	11	theme	husks	253:257	arg1	pretreatment					227:238	a new citric acid assisted hydrothermal pretreatment	187:238	a new citric acid assisted hydrothermal pretreatment	187:238	The main purpose of this work was the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery.
34606920	0	12	theme	production	101:110	arg1	extraction					55:64	the extraction	51:64	the extraction of pectin and xylooligosaccharides production from cocoa pod husks	51:131	Citric acid assisted hydrothermal pretreatment for the extraction of pectin and xylooligosaccharides production from cocoa pod husks.
34606920	0	13	from	extraction	55:64	arg1	husks					127:131	cocoa pod husks	117:131	cocoa pod husks	117:131	Citric acid assisted hydrothermal pretreatment for the extraction of pectin and xylooligosaccharides production from cocoa pod husks.
34606920	7	14	theme	value-added	1061:1071	arg1	biomolecules					1073:1084	value-added biomolecules	1061:1084	value-added biomolecules	1061:1084	Great perspectives were observed in the implementation of CPH hydrothermal pretreatment for the production of value-added biomolecules under a biorefinery concept.
34606920	3	15	theme	central	477:483	arg1	design					508:513	A central composite experimental design	475:513	A central composite experimental design	475:513	A central composite experimental design was planned to analyze the effect of pretreatment conditions.
34606920	6	16	theme	glucose	927:933	arg1	92.4 kg					916:922	92.4 kg	916:922	92.4 kg of glucose	916:933	The enzymatic conversion efficiency of the cellulosic fraction was 58.9%, leading to a production of 92.4 kg of glucose per ton of CPH.
34606920	0	17	theme	pod	123:125	arg1	husks					127:131	cocoa pod husks	117:131	cocoa pod husks	117:131	Citric acid assisted hydrothermal pretreatment for the extraction of pectin and xylooligosaccharides production from cocoa pod husks.
34606920	2	18	theme	xylooligosaccharides	386:405	arg1	production					372:381	concomitant production	360:381	concomitant production of xylooligosaccharides (XOS)	360:411	CPH́s pectin recovery was improved with concomitant production of xylooligosaccharides (XOS) through efficient enzymatic hydrolysis of the solid fraction.
34606920	2	19	theme	concomitant	360:370	arg1	production					372:381	concomitant production	360:381	concomitant production of xylooligosaccharides (XOS)	360:411	CPH́s pectin recovery was improved with concomitant production of xylooligosaccharides (XOS) through efficient enzymatic hydrolysis of the solid fraction.
34606920	0	20	theme	cocoa	117:121	arg1	husks					127:131	cocoa pod husks	117:131	cocoa pod husks	117:131	Citric acid assisted hydrothermal pretreatment for the extraction of pectin and xylooligosaccharides production from cocoa pod husks.
34606920	7	21	located	observed	975:982	arg2	perspectives					957:968	Great perspectives	951:968	Great perspectives	951:968	Great perspectives were observed in the implementation of CPH hydrothermal pretreatment for the production of value-added biomolecules under a biorefinery concept.
34606920	7	21	located	observed	975:982	arg1	implementation					991:1004	the implementation	987:1004	the implementation of CPH hydrothermal pretreatment	987:1037	Great perspectives were observed in the implementation of CPH hydrothermal pretreatment for the production of value-added biomolecules under a biorefinery concept.
34606920	2	22	theme	efficient	421:429	arg1	hydrolysis					441:450	efficient enzymatic hydrolysis	421:450	efficient enzymatic hydrolysis of the solid fraction	421:472	CPH́s pectin recovery was improved with concomitant production of xylooligosaccharides (XOS) through efficient enzymatic hydrolysis of the solid fraction.
34606920	4	23	from	w.v-1	627:631	arg1	conditions					591:600	optimal conditions	583:600	optimal conditions at 120 °C, 10 min and 2% w.v-1	583:631	Under optimal conditions at 120 °C, 10 min and 2% w.v-1, the recovery of pectin accounted for 19.3% of the biomass submitted to pretreatment with 52.2% of methyl esterification degree.
34606920	0	24	theme	Citric	0:5	arg1	acid					7:10	Citric acid	0:10	Citric acid	0:10	Citric acid assisted hydrothermal pretreatment for the extraction of pectin and xylooligosaccharides production from cocoa pod husks.
34606920	3	25	theme	pretreatment	552:563	arg1	conditions					565:574	pretreatment conditions	552:574	pretreatment conditions	552:574	A central composite experimental design was planned to analyze the effect of pretreatment conditions.
34606920	6	26	theme	enzymatic	819:827	arg1	efficiency					840:849	The enzymatic conversion efficiency	815:849	The enzymatic conversion efficiency of the cellulosic fraction	815:876	The enzymatic conversion efficiency of the cellulosic fraction was 58.9%, leading to a production of 92.4 kg of glucose per ton of CPH.
34606920	6	26	theme	enzymatic	819:827	arg1	%					886:886	58.9%	882:886	58.9%	882:886	The enzymatic conversion efficiency of the cellulosic fraction was 58.9%, leading to a production of 92.4 kg of glucose per ton of CPH.
34606920	0	27	theme	hydrothermal	21:32	arg1	pretreatment					34:45	hydrothermal pretreatment	21:45	hydrothermal pretreatment	21:45	Citric acid assisted hydrothermal pretreatment for the extraction of pectin and xylooligosaccharides production from cocoa pod husks.
34606920	6	28	theme	92.4 kg	916:922	arg1	production					902:911	a production	900:911	a production of 92.4 kg of glucose	900:933	The enzymatic conversion efficiency of the cellulosic fraction was 58.9%, leading to a production of 92.4 kg of glucose per ton of CPH.
34606920	7	29	theme	CPH	1009:1011	arg1	implementation					991:1004	the implementation	987:1004	the implementation of CPH hydrothermal pretreatment	987:1037	Great perspectives were observed in the implementation of CPH hydrothermal pretreatment for the production of value-added biomolecules under a biorefinery concept.
34606920	5	30	theme	XOS	791:793	arg1	51.9 mg.g-1					776:786	51.9 mg.g-1	776:786	51.9 mg.g-1 of XOS	776:793	Additionally, 51.9 mg.g-1 of XOS were also produced.
34606920	4	31	theme	biomass	684:690	arg1	%					675:675	19.3%	671:675	19.3% of the biomass submitted to pretreatment with 52.2% of methyl esterification degree	671:759	Under optimal conditions at 120 °C, 10 min and 2% w.v-1, the recovery of pectin accounted for 19.3% of the biomass submitted to pretreatment with 52.2% of methyl esterification degree.
34606920	4	31	theme	biomass	684:690	arg1	biomass					684:690	the biomass	680:690	the biomass submitted to pretreatment with 52.2% of methyl esterification degree	680:759	Under optimal conditions at 120 °C, 10 min and 2% w.v-1, the recovery of pectin accounted for 19.3% of the biomass submitted to pretreatment with 52.2% of methyl esterification degree.
34606920	7	32	theme	hydrothermal	1013:1024	arg1	pretreatment					1026:1037	hydrothermal pretreatment	1013:1037	CPH hydrothermal pretreatment	1009:1037	Great perspectives were observed in the implementation of CPH hydrothermal pretreatment for the production of value-added biomolecules under a biorefinery concept.
34606920	6	33	theme	conversion	829:838	arg1	efficiency					840:849	The enzymatic conversion efficiency	815:849	The enzymatic conversion efficiency of the cellulosic fraction	815:876	The enzymatic conversion efficiency of the cellulosic fraction was 58.9%, leading to a production of 92.4 kg of glucose per ton of CPH.
34606920	6	33	theme	conversion	829:838	arg1	%					886:886	58.9%	882:886	58.9%	882:886	The enzymatic conversion efficiency of the cellulosic fraction was 58.9%, leading to a production of 92.4 kg of glucose per ton of CPH.
34606920	3	34	theme	composite	485:493	arg1	design					508:513	A central composite experimental design	475:513	A central composite experimental design	475:513	A central composite experimental design was planned to analyze the effect of pretreatment conditions.
34606920	1	35	theme	new	189:191	arg1	pretreatment					227:238	a new citric acid assisted hydrothermal pretreatment	187:238	a new citric acid assisted hydrothermal pretreatment	187:238	The main purpose of this work was the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery.
34606920	4	36	theme	%	625:625	arg1	w.v-1					627:631	2% w.v-1	624:631	2% w.v-1	624:631	Under optimal conditions at 120 °C, 10 min and 2% w.v-1, the recovery of pectin accounted for 19.3% of the biomass submitted to pretreatment with 52.2% of methyl esterification degree.
34606920	4	37	from	10 min	613:618	arg1	conditions					591:600	optimal conditions	583:600	optimal conditions at 120 °C, 10 min and 2% w.v-1	583:631	Under optimal conditions at 120 °C, 10 min and 2% w.v-1, the recovery of pectin accounted for 19.3% of the biomass submitted to pretreatment with 52.2% of methyl esterification degree.
34606920	1	38	theme	citric	193:198	arg1	pretreatment					227:238	a new citric acid assisted hydrothermal pretreatment	187:238	a new citric acid assisted hydrothermal pretreatment	187:238	The main purpose of this work was the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery.
34606920	4	39	theme	pectin	650:655	arg1	recovery					638:645	the recovery	634:645	the recovery of pectin	634:655	Under optimal conditions at 120 °C, 10 min and 2% w.v-1, the recovery of pectin accounted for 19.3% of the biomass submitted to pretreatment with 52.2% of methyl esterification degree.
34606920	4	40	with	pretreatment	705:716	arg1	degree					754:759	methyl esterification degree	732:759	methyl esterification degree	732:759	Under optimal conditions at 120 °C, 10 min and 2% w.v-1, the recovery of pectin accounted for 19.3% of the biomass submitted to pretreatment with 52.2% of methyl esterification degree.
34606920	4	40	with	pretreatment	705:716	arg1	%					727:727	52.2%	723:727	52.2% of methyl esterification degree	723:759	Under optimal conditions at 120 °C, 10 min and 2% w.v-1, the recovery of pectin accounted for 19.3% of the biomass submitted to pretreatment with 52.2% of methyl esterification degree.
34606920	6	41	theme	CPH	946:948	arg1	ton					939:941	ton	939:941	ton of CPH	939:948	The enzymatic conversion efficiency of the cellulosic fraction was 58.9%, leading to a production of 92.4 kg of glucose per ton of CPH.
34606920	7	42	theme	Great	951:955	arg1	perspectives					957:968	Great perspectives	951:968	Great perspectives	951:968	Great perspectives were observed in the implementation of CPH hydrothermal pretreatment for the production of value-added biomolecules under a biorefinery concept.
34606920	1	43	theme	acid	200:203	arg1	pretreatment					227:238	a new citric acid assisted hydrothermal pretreatment	187:238	a new citric acid assisted hydrothermal pretreatment	187:238	The main purpose of this work was the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery.
34606920	4	44	from	120 °C	605:610	arg1	conditions					591:600	optimal conditions	583:600	optimal conditions at 120 °C, 10 min and 2% w.v-1	583:631	Under optimal conditions at 120 °C, 10 min and 2% w.v-1, the recovery of pectin accounted for 19.3% of the biomass submitted to pretreatment with 52.2% of methyl esterification degree.
34606920	3	45	theme	conditions	565:574	arg1	effect					542:547	the effect	538:547	the effect of pretreatment conditions	538:574	A central composite experimental design was planned to analyze the effect of pretreatment conditions.
34606920	2	46	theme	enzymatic	431:439	arg1	hydrolysis					441:450	efficient enzymatic hydrolysis	421:450	efficient enzymatic hydrolysis of the solid fraction	421:472	CPH́s pectin recovery was improved with concomitant production of xylooligosaccharides (XOS) through efficient enzymatic hydrolysis of the solid fraction.
34606920	7	47	theme	biorefinery	1094:1104	arg1	concept					1106:1112	a biorefinery concept	1092:1112	a biorefinery concept	1092:1112	Great perspectives were observed in the implementation of CPH hydrothermal pretreatment for the production of value-added biomolecules under a biorefinery concept.
34606920	4	48	theme	esterification	739:752	arg1	degree					754:759	methyl esterification degree	732:759	methyl esterification degree	732:759	Under optimal conditions at 120 °C, 10 min and 2% w.v-1, the recovery of pectin accounted for 19.3% of the biomass submitted to pretreatment with 52.2% of methyl esterification degree.
34606920	1	49	theme	assisted	205:212	arg1	pretreatment					227:238	a new citric acid assisted hydrothermal pretreatment	187:238	a new citric acid assisted hydrothermal pretreatment	187:238	The main purpose of this work was the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery.
34606920	4	50	theme	methyl	732:737	arg1	esterification					739:752	methyl esterification	732:752	methyl esterification degree	732:759	Under optimal conditions at 120 °C, 10 min and 2% w.v-1, the recovery of pectin accounted for 19.3% of the biomass submitted to pretreatment with 52.2% of methyl esterification degree.
34606920	7	51	dep	CPH	1009:1011	arg1	pretreatment					1026:1037	hydrothermal pretreatment	1013:1037	CPH hydrothermal pretreatment	1009:1037	Great perspectives were observed in the implementation of CPH hydrothermal pretreatment for the production of value-added biomolecules under a biorefinery concept.
34606920	1	52	theme	main	138:141	arg1	development					172:182	the development	168:182	the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery	168:317	The main purpose of this work was the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery.
34606920	1	52	theme	main	138:141	arg1	purpose					143:149	The main purpose	134:149	The main purpose of this work	134:162	The main purpose of this work was the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery.
34606920	1	53	theme	hydrothermal	214:225	arg1	pretreatment					227:238	a new citric acid assisted hydrothermal pretreatment	187:238	a new citric acid assisted hydrothermal pretreatment	187:238	The main purpose of this work was the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery.
34606920	0	54	theme	pectin	69:74	arg1	production					101:110	pectin and xylooligosaccharides production	69:110	pectin and xylooligosaccharides production from cocoa pod husks	69:131	Citric acid assisted hydrothermal pretreatment for the extraction of pectin and xylooligosaccharides production from cocoa pod husks.
34606920	4	55	theme	2	624:624	arg1	%					625:625	%	625:625	%	625:625	Under optimal conditions at 120 °C, 10 min and 2% w.v-1, the recovery of pectin accounted for 19.3% of the biomass submitted to pretreatment with 52.2% of methyl esterification degree.
34606920	3	56	theme	experimental	495:506	arg1	design					508:513	A central composite experimental design	475:513	A central composite experimental design	475:513	A central composite experimental design was planned to analyze the effect of pretreatment conditions.
34606920	1	57	theme	pretreatment	227:238	arg1	development					172:182	the development	168:182	the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery	168:317	The main purpose of this work was the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery.
34606920	1	57	theme	pretreatment	227:238	arg1	purpose					143:149	The main purpose	134:149	The main purpose of this work	134:162	The main purpose of this work was the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery.
34606920	4	58	theme	optimal	583:589	arg1	conditions					591:600	optimal conditions	583:600	optimal conditions at 120 °C, 10 min and 2% w.v-1	583:631	Under optimal conditions at 120 °C, 10 min and 2% w.v-1, the recovery of pectin accounted for 19.3% of the biomass submitted to pretreatment with 52.2% of methyl esterification degree.
34606920	4	59	theme	degree	754:759	arg1	degree					754:759	methyl esterification degree	732:759	methyl esterification degree	732:759	Under optimal conditions at 120 °C, 10 min and 2% w.v-1, the recovery of pectin accounted for 19.3% of the biomass submitted to pretreatment with 52.2% of methyl esterification degree.
34606920	4	59	theme	degree	754:759	arg1	%					727:727	52.2%	723:727	52.2% of methyl esterification degree	723:759	Under optimal conditions at 120 °C, 10 min and 2% w.v-1, the recovery of pectin accounted for 19.3% of the biomass submitted to pretreatment with 52.2% of methyl esterification degree.
34606920	2	60	theme	pectin	326:331	arg1	recovery					333:340	CPH́s pectin recovery	320:340	CPH́s pectin recovery	320:340	CPH́s pectin recovery was improved with concomitant production of xylooligosaccharides (XOS) through efficient enzymatic hydrolysis of the solid fraction.
34606920	0	61	from	husks	127:131	arg1	production					101:110	pectin and xylooligosaccharides production	69:110	pectin and xylooligosaccharides production from cocoa pod husks	69:131	Citric acid assisted hydrothermal pretreatment for the extraction of pectin and xylooligosaccharides production from cocoa pod husks.
34606920	0	61	from	husks	127:131	arg1	extraction					55:64	the extraction	51:64	the extraction of pectin and xylooligosaccharides production from cocoa pod husks	51:131	Citric acid assisted hydrothermal pretreatment for the extraction of pectin and xylooligosaccharides production from cocoa pod husks.
34325297	4	0	theme	UV	510:511	arg1	protection					513:522	UV protection	510:522	UV protection	510:522	The integration of ZnSNP has greatly improved the physical properties of the film, such as mechanical and UV protection properties, without significantly changing the transparency of the film.
34325297	0	1	theme	packaging	102:110	arg1	applications					112:123	active packaging applications	95:123	active packaging applications	95:123	Preparation of pectin/agar-based functional films integrated with zinc sulfide nano petals for active packaging applications.
34325297	0	2	theme	active	95:100	arg1	applications					112:123	active packaging applications	95:123	active packaging applications	95:123	Preparation of pectin/agar-based functional films integrated with zinc sulfide nano petals for active packaging applications.
34325297	4	3	theme	film	481:484	arg1	properties					463:472	the physical properties	450:472	the physical properties	450:472	The integration of ZnSNP has greatly improved the physical properties of the film, such as mechanical and UV protection properties, without significantly changing the transparency of the film.
34325297	4	3	theme	film	481:484	arg1	properties					524:533	mechanical and UV protection properties	495:533	mechanical and UV protection properties	495:533	The integration of ZnSNP has greatly improved the physical properties of the film, such as mechanical and UV protection properties, without significantly changing the transparency of the film.
34325297	7	4	theme	functional	856:865	arg1	films					885:889	The functional bio-nanocomposite films	852:889	The functional bio-nanocomposite films based on pectin/agar	852:910	The functional bio-nanocomposite films based on pectin/agar have high potential in active packaging applications.
34325297	4	5	theme	film	591:594	arg1	transparency					571:582	the transparency	567:582	the transparency of the film	567:594	The integration of ZnSNP has greatly improved the physical properties of the film, such as mechanical and UV protection properties, without significantly changing the transparency of the film.
34325297	5	6	theme	nanofillers	617:627	arg1	addition					601:608	The addition	597:608	The addition of the nanofillers	597:627	The addition of the nanofillers did not affect the film's hydrophobicity, water vapor barrier, and thermal properties.
34325297	6	7	theme	pathogenic	801:810	arg1	bacteria					812:819	foodborne pathogenic bacteria	791:819	foodborne pathogenic bacteria	791:819	Moreover, the composite film showed intense antibacterial activity against foodborne pathogenic bacteria, E. coli and L. monocytogenes.
34325297	6	7	theme	pathogenic	801:810	arg1	coli					825:828	E. coli	822:828	E. coli	822:828	Moreover, the composite film showed intense antibacterial activity against foodborne pathogenic bacteria, E. coli and L. monocytogenes.
34325297	6	7	theme	pathogenic	801:810	arg1	monocytogenes					837:849	L. monocytogenes	834:849	L. monocytogenes	834:849	Moreover, the composite film showed intense antibacterial activity against foodborne pathogenic bacteria, E. coli and L. monocytogenes.
34325297	7	8	theme	bio-nanocomposite	867:883	arg1	films					885:889	The functional bio-nanocomposite films	852:889	The functional bio-nanocomposite films based on pectin/agar	852:910	The functional bio-nanocomposite films based on pectin/agar have high potential in active packaging applications.
34325297	1	9	theme	zinc	168:171	arg1	nanoparticles					181:193	zinc sulfide nanoparticles	168:193	zinc sulfide nanoparticles (ZnSNP) using a simple one-pot reaction	168:233	Here we report on the robust synthesis of zinc sulfide nanoparticles (ZnSNP) using a simple one-pot reaction.
34325297	1	9	theme	zinc	168:171	arg1	ZnSNP					196:200	ZnSNP	196:200	ZnSNP	196:200	Here we report on the robust synthesis of zinc sulfide nanoparticles (ZnSNP) using a simple one-pot reaction.
34325297	7	10	contain	have	912:915	arg1	films					885:889	The functional bio-nanocomposite films	852:889	The functional bio-nanocomposite films based on pectin/agar	852:910	The functional bio-nanocomposite films based on pectin/agar have high potential in active packaging applications.
34325297	7	10	contain	have	912:915	arg2	potential					922:930	high potential	917:930	high potential	917:930	The functional bio-nanocomposite films based on pectin/agar have high potential in active packaging applications.
34325297	6	11	theme	foodborne	791:799	arg1	bacteria					812:819	foodborne pathogenic bacteria	791:819	foodborne pathogenic bacteria	791:819	Moreover, the composite film showed intense antibacterial activity against foodborne pathogenic bacteria, E. coli and L. monocytogenes.
34325297	6	11	theme	foodborne	791:799	arg1	coli					825:828	E. coli	822:828	E. coli	822:828	Moreover, the composite film showed intense antibacterial activity against foodborne pathogenic bacteria, E. coli and L. monocytogenes.
34325297	6	11	theme	foodborne	791:799	arg1	monocytogenes					837:849	L. monocytogenes	834:849	L. monocytogenes	834:849	Moreover, the composite film showed intense antibacterial activity against foodborne pathogenic bacteria, E. coli and L. monocytogenes.
34325297	1	12	theme	sulfide	173:179	arg1	nanoparticles					181:193	zinc sulfide nanoparticles	168:193	zinc sulfide nanoparticles (ZnSNP) using a simple one-pot reaction	168:233	Here we report on the robust synthesis of zinc sulfide nanoparticles (ZnSNP) using a simple one-pot reaction.
34325297	1	12	theme	sulfide	173:179	arg1	ZnSNP					196:200	ZnSNP	196:200	ZnSNP	196:200	Here we report on the robust synthesis of zinc sulfide nanoparticles (ZnSNP) using a simple one-pot reaction.
34325297	3	13	theme	composite	388:396	arg1	film					398:401	the pectin/agar-based functional composite film	355:401	the pectin/agar-based functional composite film	355:401	The ZnSNP was added to fabricate the pectin/agar-based functional composite film.
34325297	1	14	theme	nanoparticles	181:193	arg1	synthesis					155:163	the robust synthesis	144:163	the robust synthesis of zinc sulfide nanoparticles (ZnSNP) using a simple one-pot reaction	144:233	Here we report on the robust synthesis of zinc sulfide nanoparticles (ZnSNP) using a simple one-pot reaction.
34325297	7	15	theme	active	935:940	arg1	applications					952:963	active packaging applications	935:963	active packaging applications	935:963	The functional bio-nanocomposite films based on pectin/agar have high potential in active packaging applications.
34325297	2	16	theme	petal-shaped	295:306	arg1	ZnSNP					249:253	The prepared ZnSNP	236:253	The prepared ZnSNP	236:253	The prepared ZnSNP was characterized and confirmed to be a petal-shaped nanoparticle.
34325297	2	16	theme	petal-shaped	295:306	arg1	nanoparticle					308:319	a petal-shaped nanoparticle	293:319	a petal-shaped nanoparticle	293:319	The prepared ZnSNP was characterized and confirmed to be a petal-shaped nanoparticle.
34325297	3	17	theme	pectin/agar-based	359:375	arg1	film					398:401	the pectin/agar-based functional composite film	355:401	the pectin/agar-based functional composite film	355:401	The ZnSNP was added to fabricate the pectin/agar-based functional composite film.
34325297	6	18	theme	antibacterial	760:772	arg1	activity					774:781	intense antibacterial activity	752:781	intense antibacterial activity against foodborne pathogenic bacteria, E. coli and L. monocytogenes	752:849	Moreover, the composite film showed intense antibacterial activity against foodborne pathogenic bacteria, E. coli and L. monocytogenes.
34325297	3	19	theme	functional	377:386	arg1	film					398:401	the pectin/agar-based functional composite film	355:401	the pectin/agar-based functional composite film	355:401	The ZnSNP was added to fabricate the pectin/agar-based functional composite film.
34325297	4	20	theme	physical	454:461	arg1	properties					463:472	the physical properties	450:472	the physical properties	450:472	The integration of ZnSNP has greatly improved the physical properties of the film, such as mechanical and UV protection properties, without significantly changing the transparency of the film.
34325297	4	20	theme	physical	454:461	arg1	properties					524:533	mechanical and UV protection properties	495:533	mechanical and UV protection properties	495:533	The integration of ZnSNP has greatly improved the physical properties of the film, such as mechanical and UV protection properties, without significantly changing the transparency of the film.
34325297	5	21	theme	thermal	696:702	arg1	properties					704:713	thermal properties	696:713	thermal properties	696:713	The addition of the nanofillers did not affect the film's hydrophobicity, water vapor barrier, and thermal properties.
34325297	0	22	theme	films	44:48	arg1	Preparation					0:10	Preparation	0:10	Preparation of pectin/agar-based functional films	0:48	Preparation of pectin/agar-based functional films integrated with zinc sulfide nano petals for active packaging applications.
34325297	0	23	theme	functional	33:42	arg1	films					44:48	pectin/agar-based functional films	15:48	pectin/agar-based functional films	15:48	Preparation of pectin/agar-based functional films integrated with zinc sulfide nano petals for active packaging applications.
34325297	6	24	theme	intense	752:758	arg1	activity					774:781	intense antibacterial activity	752:781	intense antibacterial activity against foodborne pathogenic bacteria, E. coli and L. monocytogenes	752:849	Moreover, the composite film showed intense antibacterial activity against foodborne pathogenic bacteria, E. coli and L. monocytogenes.
34325297	0	25	theme	zinc	66:69	arg1	petals					84:89	zinc sulfide nano petals	66:89	zinc sulfide nano petals for active packaging applications	66:123	Preparation of pectin/agar-based functional films integrated with zinc sulfide nano petals for active packaging applications.
34325297	4	26	theme	protection	513:522	arg1	properties					524:533	mechanical and UV protection properties	495:533	mechanical and UV protection properties	495:533	The integration of ZnSNP has greatly improved the physical properties of the film, such as mechanical and UV protection properties, without significantly changing the transparency of the film.
34325297	5	27	theme	water	671:675	arg1	barrier					683:689	water vapor barrier	671:689	water vapor barrier	671:689	The addition of the nanofillers did not affect the film's hydrophobicity, water vapor barrier, and thermal properties.
34325297	7	28	theme	packaging	942:950	arg1	applications					952:963	active packaging applications	935:963	active packaging applications	935:963	The functional bio-nanocomposite films based on pectin/agar have high potential in active packaging applications.
34325297	5	29	theme	vapor	677:681	arg1	barrier					683:689	water vapor barrier	671:689	water vapor barrier	671:689	The addition of the nanofillers did not affect the film's hydrophobicity, water vapor barrier, and thermal properties.
34325297	4	30	theme	ZnSNP	423:427	arg1	integration					408:418	The integration	404:418	The integration of ZnSNP	404:427	The integration of ZnSNP has greatly improved the physical properties of the film, such as mechanical and UV protection properties, without significantly changing the transparency of the film.
34325297	6	31	theme	composite	730:738	arg1	film					740:743	the composite film	726:743	the composite film	726:743	Moreover, the composite film showed intense antibacterial activity against foodborne pathogenic bacteria, E. coli and L. monocytogenes.
34325297	1	32	theme	simple	211:216	arg1	reaction					226:233	a simple one-pot reaction	209:233	a simple one-pot reaction	209:233	Here we report on the robust synthesis of zinc sulfide nanoparticles (ZnSNP) using a simple one-pot reaction.
34325297	1	33	theme	one-pot	218:224	arg1	reaction					226:233	a simple one-pot reaction	209:233	a simple one-pot reaction	209:233	Here we report on the robust synthesis of zinc sulfide nanoparticles (ZnSNP) using a simple one-pot reaction.
34325297	0	34	theme	nano	79:82	arg1	petals					84:89	zinc sulfide nano petals	66:89	zinc sulfide nano petals for active packaging applications	66:123	Preparation of pectin/agar-based functional films integrated with zinc sulfide nano petals for active packaging applications.
34325297	2	35	theme	prepared	240:247	arg1	ZnSNP					249:253	The prepared ZnSNP	236:253	The prepared ZnSNP	236:253	The prepared ZnSNP was characterized and confirmed to be a petal-shaped nanoparticle.
34325297	2	35	theme	prepared	240:247	arg1	nanoparticle					308:319	a petal-shaped nanoparticle	293:319	a petal-shaped nanoparticle	293:319	The prepared ZnSNP was characterized and confirmed to be a petal-shaped nanoparticle.
34325297	7	36	theme	high	917:920	arg1	potential					922:930	high potential	917:930	high potential	917:930	The functional bio-nanocomposite films based on pectin/agar have high potential in active packaging applications.
34325297	0	37	theme	sulfide	71:77	arg1	petals					84:89	zinc sulfide nano petals	66:89	zinc sulfide nano petals for active packaging applications	66:123	Preparation of pectin/agar-based functional films integrated with zinc sulfide nano petals for active packaging applications.
34325297	4	38	theme	mechanical	495:504	arg1	properties					524:533	mechanical and UV protection properties	495:533	mechanical and UV protection properties	495:533	The integration of ZnSNP has greatly improved the physical properties of the film, such as mechanical and UV protection properties, without significantly changing the transparency of the film.
34325297	0	39	theme	pectin/agar-based	15:31	arg1	films					44:48	pectin/agar-based functional films	15:48	pectin/agar-based functional films	15:48	Preparation of pectin/agar-based functional films integrated with zinc sulfide nano petals for active packaging applications.
34325297	1	40	theme	robust	148:153	arg1	synthesis					155:163	the robust synthesis	144:163	the robust synthesis of zinc sulfide nanoparticles (ZnSNP) using a simple one-pot reaction	144:233	Here we report on the robust synthesis of zinc sulfide nanoparticles (ZnSNP) using a simple one-pot reaction.
32332833	2	0	theme	tolerant	514:521	arg1	enzymes					523:529	glucose tolerant enzymes	506:529	glucose tolerant enzymes	506:529	They catalyze the rate-limiting reaction in the conversion of cellobiose to glucose in the saccharification of cellulose for second-generation ethanol production, and due to this important role the search for glucose tolerant enzymes is of biochemical and biotechnological importance.
32332833	1	1	theme	aryl	258:261	arg1	β-D-glucosides					263:276	alkyl and aryl β-D-glucosides	248:276	β-D-glucosides	263:276	β-glucosidases catalyze the hydrolysis β-1,4, β-1,3 and β-1,6 glucosidic linkages from non-reducing end of short chain oligosaccharides, alkyl and aryl β-D-glucosides and disaccharides.
32332833	4	2	theme	many	864:867	arg1	Bgls					869:872	many Bgls	864:872	many Bgls that are completely inhibited by glucose	864:913	Kinetic characterization revealed that the MpBgl3 was highly tolerant to glucose, which is in contrast to many Bgls that are completely inhibited by glucose.
32332833	1	3	theme	β-D-glucosides	263:276	arg1	end					211:213	non-reducing end	198:213	non-reducing end of short chain oligosaccharides, alkyl and aryl β-D-glucosides and disaccharides	198:294	β-glucosidases catalyze the hydrolysis β-1,4, β-1,3 and β-1,6 glucosidic linkages from non-reducing end of short chain oligosaccharides, alkyl and aryl β-D-glucosides and disaccharides.
32332833	2	4	theme	biotechnological	553:568	arg1	importance					570:579	biochemical and biotechnological importance	537:579	biochemical and biotechnological importance	537:579	They catalyze the rate-limiting reaction in the conversion of cellobiose to glucose in the saccharification of cellulose for second-generation ethanol production, and due to this important role the search for glucose tolerant enzymes is of biochemical and biotechnological importance.
32332833	5	5	theme	differences	1015:1025	arg1	evaluation					990:999	the evaluation	986:999	the evaluation of structural differences with a Bgl3 that is inhibited by glucose	986:1066	A 3D model of MpBgl3 was generated by molecular modeling and used for the evaluation of structural differences with a Bgl3 that is inhibited by glucose.
32332833	0	6	theme	Cellulose	83:91	arg1	Saccharification					93:108	Cellulose Saccharification	83:108	Cellulose Saccharification	83:108	A Highly Glucose Tolerant ß-Glucosidase from Malbranchea pulchella (MpBg3) Enables Cellulose Saccharification.
32332833	0	7	from	pulchella	57:65	arg1	ß-Glucosidase					26:38	A Highly Glucose Tolerant ß-Glucosidase	0:38	A Highly Glucose Tolerant ß-Glucosidase from Malbranchea pulchella (MpBg3)	0:73	A Highly Glucose Tolerant ß-Glucosidase from Malbranchea pulchella (MpBg3) Enables Cellulose Saccharification.
32332833	6	8	theme	GH3	1154:1156	arg1	β-glucosidases					1158:1171	GH3 β-glucosidases	1154:1171	GH3 β-glucosidases	1154:1171	Taken together, our results provide new clues to understand the glucose tolerance in GH3 β-glucosidases.
32332833	5	9	theme	molecular	954:962	arg1	modeling					964:971	molecular modeling	954:971	molecular modeling	954:971	A 3D model of MpBgl3 was generated by molecular modeling and used for the evaluation of structural differences with a Bgl3 that is inhibited by glucose.
32332833	1	10	theme	disaccharides	282:294	arg1	end					211:213	non-reducing end	198:213	non-reducing end of short chain oligosaccharides, alkyl and aryl β-D-glucosides and disaccharides	198:294	β-glucosidases catalyze the hydrolysis β-1,4, β-1,3 and β-1,6 glucosidic linkages from non-reducing end of short chain oligosaccharides, alkyl and aryl β-D-glucosides and disaccharides.
32332833	2	11	theme	biochemical	537:547	arg1	importance					570:579	biochemical and biotechnological importance	537:579	biochemical and biotechnological importance	537:579	They catalyze the rate-limiting reaction in the conversion of cellobiose to glucose in the saccharification of cellulose for second-generation ethanol production, and due to this important role the search for glucose tolerant enzymes is of biochemical and biotechnological importance.
32332833	3	12	theme	sole	738:741	arg1	source					750:755	the sole carbon source	734:755	the sole carbon source	734:755	In this study we characterize a family 3 glycosyl hydrolase (GH3) β-glucosidase (Bgl) produced by Malbranchea pulchella (MpBgl3) grown on cellobiose as the sole carbon source.
32332833	6	13	from	tolerance	1141:1149	arg1	β-glucosidases					1158:1171	GH3 β-glucosidases	1154:1171	GH3 β-glucosidases	1154:1171	Taken together, our results provide new clues to understand the glucose tolerance in GH3 β-glucosidases.
32332833	1	14	theme	glucosidic	173:182	arg1	linkages					184:191	β-1,6 glucosidic linkages	167:191	β-1,6 glucosidic linkages from non-reducing end of short chain oligosaccharides, alkyl and aryl β-D-glucosides and disaccharides	167:294	β-glucosidases catalyze the hydrolysis β-1,4, β-1,3 and β-1,6 glucosidic linkages from non-reducing end of short chain oligosaccharides, alkyl and aryl β-D-glucosides and disaccharides.
32332833	2	15	theme	important	476:484	arg1	role					486:489	this important role	471:489	this important role	471:489	They catalyze the rate-limiting reaction in the conversion of cellobiose to glucose in the saccharification of cellulose for second-generation ethanol production, and due to this important role the search for glucose tolerant enzymes is of biochemical and biotechnological importance.
32332833	6	16	theme	glucose	1133:1139	arg1	tolerance					1141:1149	the glucose tolerance	1129:1149	the glucose tolerance in GH3 β-glucosidases	1129:1171	Taken together, our results provide new clues to understand the glucose tolerance in GH3 β-glucosidases.
32332833	2	17	theme	cellulose	408:416	arg1	saccharification					388:403	the saccharification	384:403	the saccharification of cellulose for second-generation ethanol production	384:457	They catalyze the rate-limiting reaction in the conversion of cellobiose to glucose in the saccharification of cellulose for second-generation ethanol production, and due to this important role the search for glucose tolerant enzymes is of biochemical and biotechnological importance.
32332833	3	18	theme	Malbranchea	680:690	arg1	MpBgl3					703:708	MpBgl3	703:708	MpBgl3	703:708	In this study we characterize a family 3 glycosyl hydrolase (GH3) β-glucosidase (Bgl) produced by Malbranchea pulchella (MpBgl3) grown on cellobiose as the sole carbon source.
32332833	3	18	theme	Malbranchea	680:690	arg1	pulchella					692:700	Malbranchea pulchella	680:700	Malbranchea pulchella (MpBgl3) grown on cellobiose as the sole carbon source	680:755	In this study we characterize a family 3 glycosyl hydrolase (GH3) β-glucosidase (Bgl) produced by Malbranchea pulchella (MpBgl3) grown on cellobiose as the sole carbon source.
32332833	5	19	theme	MpBgl3	930:935	arg1	model					921:925	A 3D model	916:925	A 3D model of MpBgl3	916:935	A 3D model of MpBgl3 was generated by molecular modeling and used for the evaluation of structural differences with a Bgl3 that is inhibited by glucose.
32332833	1	20	theme	non-reducing	198:209	arg1	end					211:213	non-reducing end	198:213	non-reducing end of short chain oligosaccharides, alkyl and aryl β-D-glucosides and disaccharides	198:294	β-glucosidases catalyze the hydrolysis β-1,4, β-1,3 and β-1,6 glucosidic linkages from non-reducing end of short chain oligosaccharides, alkyl and aryl β-D-glucosides and disaccharides.
32332833	0	21	theme	Tolerant	17:24	arg1	ß-Glucosidase					26:38	A Highly Glucose Tolerant ß-Glucosidase	0:38	A Highly Glucose Tolerant ß-Glucosidase from Malbranchea pulchella (MpBg3)	0:73	A Highly Glucose Tolerant ß-Glucosidase from Malbranchea pulchella (MpBg3) Enables Cellulose Saccharification.
32332833	4	22	theme	Kinetic	758:764	arg1	characterization					766:781	Kinetic characterization	758:781	Kinetic characterization	758:781	Kinetic characterization revealed that the MpBgl3 was highly tolerant to glucose, which is in contrast to many Bgls that are completely inhibited by glucose.
32332833	3	23	theme	carbon	743:748	arg1	source					750:755	the sole carbon source	734:755	the sole carbon source	734:755	In this study we characterize a family 3 glycosyl hydrolase (GH3) β-glucosidase (Bgl) produced by Malbranchea pulchella (MpBgl3) grown on cellobiose as the sole carbon source.
32332833	2	24	theme	rate-limiting	315:327	arg1	reaction					329:336	the rate-limiting reaction	311:336	the rate-limiting reaction in the conversion of cellobiose to glucose in the saccharification of cellulose for second-generation ethanol production	311:457	They catalyze the rate-limiting reaction in the conversion of cellobiose to glucose in the saccharification of cellulose for second-generation ethanol production, and due to this important role the search for glucose tolerant enzymes is of biochemical and biotechnological importance.
32332833	6	25	theme	new	1105:1107	arg1	clues					1109:1113	new clues	1105:1113	new clues	1105:1113	Taken together, our results provide new clues to understand the glucose tolerance in GH3 β-glucosidases.
32332833	3	26	theme	glycosyl	623:630	arg1	Bgl					663:665	Bgl	663:665	Bgl	663:665	In this study we characterize a family 3 glycosyl hydrolase (GH3) β-glucosidase (Bgl) produced by Malbranchea pulchella (MpBgl3) grown on cellobiose as the sole carbon source.
32332833	3	26	theme	glycosyl	623:630	arg1	β-glucosidase					648:660	a family 3 glycosyl hydrolase (GH3) β-glucosidase	612:660	a family 3 glycosyl hydrolase (GH3) β-glucosidase (Bgl) produced by Malbranchea pulchella (MpBgl3) grown on cellobiose as the sole carbon source	612:755	In this study we characterize a family 3 glycosyl hydrolase (GH3) β-glucosidase (Bgl) produced by Malbranchea pulchella (MpBgl3) grown on cellobiose as the sole carbon source.
32332833	1	27	theme	short	218:222	arg1	oligosaccharides					230:245	short chain oligosaccharides	218:245	short chain oligosaccharides	218:245	β-glucosidases catalyze the hydrolysis β-1,4, β-1,3 and β-1,6 glucosidic linkages from non-reducing end of short chain oligosaccharides, alkyl and aryl β-D-glucosides and disaccharides.
32332833	2	28	theme	second-generation	422:438	arg1	production					448:457	second-generation ethanol production	422:457	second-generation ethanol production	422:457	They catalyze the rate-limiting reaction in the conversion of cellobiose to glucose in the saccharification of cellulose for second-generation ethanol production, and due to this important role the search for glucose tolerant enzymes is of biochemical and biotechnological importance.
32332833	3	29	theme	hydrolase	632:640	arg1	Bgl					663:665	Bgl	663:665	Bgl	663:665	In this study we characterize a family 3 glycosyl hydrolase (GH3) β-glucosidase (Bgl) produced by Malbranchea pulchella (MpBgl3) grown on cellobiose as the sole carbon source.
32332833	3	29	theme	hydrolase	632:640	arg1	β-glucosidase					648:660	a family 3 glycosyl hydrolase (GH3) β-glucosidase	612:660	a family 3 glycosyl hydrolase (GH3) β-glucosidase (Bgl) produced by Malbranchea pulchella (MpBgl3) grown on cellobiose as the sole carbon source	612:755	In this study we characterize a family 3 glycosyl hydrolase (GH3) β-glucosidase (Bgl) produced by Malbranchea pulchella (MpBgl3) grown on cellobiose as the sole carbon source.
32332833	1	30	theme	chain	224:228	arg1	oligosaccharides					230:245	short chain oligosaccharides	218:245	short chain oligosaccharides	218:245	β-glucosidases catalyze the hydrolysis β-1,4, β-1,3 and β-1,6 glucosidic linkages from non-reducing end of short chain oligosaccharides, alkyl and aryl β-D-glucosides and disaccharides.
32332833	4	31	dep	Bgls	869:872	arg1	contrast					852:859	contrast	852:859	contrast	852:859	Kinetic characterization revealed that the MpBgl3 was highly tolerant to glucose, which is in contrast to many Bgls that are completely inhibited by glucose.
32332833	5	32	with	differences	1015:1025	arg1	Bgl3					1034:1037	a Bgl3	1032:1037	a Bgl3 that is inhibited by glucose	1032:1066	A 3D model of MpBgl3 was generated by molecular modeling and used for the evaluation of structural differences with a Bgl3 that is inhibited by glucose.
32332833	3	33	theme	family	614:619	arg1	Bgl					663:665	Bgl	663:665	Bgl	663:665	In this study we characterize a family 3 glycosyl hydrolase (GH3) β-glucosidase (Bgl) produced by Malbranchea pulchella (MpBgl3) grown on cellobiose as the sole carbon source.
32332833	3	33	theme	family	614:619	arg1	β-glucosidase					648:660	a family 3 glycosyl hydrolase (GH3) β-glucosidase	612:660	a family 3 glycosyl hydrolase (GH3) β-glucosidase (Bgl) produced by Malbranchea pulchella (MpBgl3) grown on cellobiose as the sole carbon source	612:755	In this study we characterize a family 3 glycosyl hydrolase (GH3) β-glucosidase (Bgl) produced by Malbranchea pulchella (MpBgl3) grown on cellobiose as the sole carbon source.
32332833	1	34	from	end	211:213	arg1	hydrolysis					139:148	the hydrolysis β-1,4, β-1,3	135:161	hydrolysis	139:148	β-glucosidases catalyze the hydrolysis β-1,4, β-1,3 and β-1,6 glucosidic linkages from non-reducing end of short chain oligosaccharides, alkyl and aryl β-D-glucosides and disaccharides.
32332833	1	34	from	end	211:213	arg1	linkages					184:191	β-1,6 glucosidic linkages	167:191	β-1,6 glucosidic linkages from non-reducing end of short chain oligosaccharides, alkyl and aryl β-D-glucosides and disaccharides	167:294	β-glucosidases catalyze the hydrolysis β-1,4, β-1,3 and β-1,6 glucosidic linkages from non-reducing end of short chain oligosaccharides, alkyl and aryl β-D-glucosides and disaccharides.
32332833	2	35	theme	cellobiose	359:368	arg1	conversion					345:354	the conversion	341:354	the conversion of cellobiose to glucose	341:379	They catalyze the rate-limiting reaction in the conversion of cellobiose to glucose in the saccharification of cellulose for second-generation ethanol production, and due to this important role the search for glucose tolerant enzymes is of biochemical and biotechnological importance.
32332833	5	36	theme	structural	1004:1013	arg1	differences					1015:1025	structural differences	1004:1025	structural differences with a Bgl3 that is inhibited by glucose	1004:1066	A 3D model of MpBgl3 was generated by molecular modeling and used for the evaluation of structural differences with a Bgl3 that is inhibited by glucose.
32332833	5	37	theme	3D	918:919	arg1	model					921:925	A 3D model	916:925	A 3D model of MpBgl3	916:935	A 3D model of MpBgl3 was generated by molecular modeling and used for the evaluation of structural differences with a Bgl3 that is inhibited by glucose.
32332833	1	38	theme	oligosaccharides	230:245	arg1	end					211:213	non-reducing end	198:213	non-reducing end of short chain oligosaccharides, alkyl and aryl β-D-glucosides and disaccharides	198:294	β-glucosidases catalyze the hydrolysis β-1,4, β-1,3 and β-1,6 glucosidic linkages from non-reducing end of short chain oligosaccharides, alkyl and aryl β-D-glucosides and disaccharides.
32332833	5	39	used	used	977:980	arg2	model					921:925	A 3D model	916:925	A 3D model of MpBgl3	916:935	A 3D model of MpBgl3 was generated by molecular modeling and used for the evaluation of structural differences with a Bgl3 that is inhibited by glucose.
32332833	2	40	theme	ethanol	440:446	arg1	production					448:457	second-generation ethanol production	422:457	second-generation ethanol production	422:457	They catalyze the rate-limiting reaction in the conversion of cellobiose to glucose in the saccharification of cellulose for second-generation ethanol production, and due to this important role the search for glucose tolerant enzymes is of biochemical and biotechnological importance.
32332833	1	41	theme	alkyl	248:252	arg1	β-D-glucosides					263:276	alkyl and aryl β-D-glucosides	248:276	β-D-glucosides	263:276	β-glucosidases catalyze the hydrolysis β-1,4, β-1,3 and β-1,6 glucosidic linkages from non-reducing end of short chain oligosaccharides, alkyl and aryl β-D-glucosides and disaccharides.
32332833	3	42	theme	GH3	643:645	arg1	Bgl					663:665	Bgl	663:665	Bgl	663:665	In this study we characterize a family 3 glycosyl hydrolase (GH3) β-glucosidase (Bgl) produced by Malbranchea pulchella (MpBgl3) grown on cellobiose as the sole carbon source.
32332833	3	42	theme	GH3	643:645	arg1	β-glucosidase					648:660	a family 3 glycosyl hydrolase (GH3) β-glucosidase	612:660	a family 3 glycosyl hydrolase (GH3) β-glucosidase (Bgl) produced by Malbranchea pulchella (MpBgl3) grown on cellobiose as the sole carbon source	612:755	In this study we characterize a family 3 glycosyl hydrolase (GH3) β-glucosidase (Bgl) produced by Malbranchea pulchella (MpBgl3) grown on cellobiose as the sole carbon source.
32332833	2	43	from	reaction	329:336	arg1	conversion					345:354	the conversion	341:354	the conversion of cellobiose to glucose	341:379	They catalyze the rate-limiting reaction in the conversion of cellobiose to glucose in the saccharification of cellulose for second-generation ethanol production, and due to this important role the search for glucose tolerant enzymes is of biochemical and biotechnological importance.
32332833	2	43	from	reaction	329:336	arg1	saccharification					388:403	the saccharification	384:403	the saccharification of cellulose for second-generation ethanol production	384:457	They catalyze the rate-limiting reaction in the conversion of cellobiose to glucose in the saccharification of cellulose for second-generation ethanol production, and due to this important role the search for glucose tolerant enzymes is of biochemical and biotechnological importance.
34920058	5	0	theme	composites	757:766	arg1	characterization					724:739	The characterization	720:739	The characterization of the chitosan composites	720:766	The characterization of the chitosan composites has been thoroughly studied.
34920058	8	1	theme	phosphorus	1049:1058	arg1	release					1060:1066	phosphorus release	1049:1066	phosphorus release from coated NPK/CS-Gl-K granules	1049:1099	Moreover, phosphorus release from coated NPK/CS-Gl-K granules was generally delayed contrasted with NPK/uncoated.
34920058	6	2	theme	chitosan-kaolinite	877:894	arg1	composites					896:905	chitosan-kaolinite composites	877:905	chitosan-kaolinite composites	877:905	The NPK mineral fertilizer was coated according to the dip-immersing process of chitosan-kaolinite composites.
34920058	1	3	theme	slow-release	157:168	arg1	fertilizers					170:180	slow-release fertilizers	157:180	slow-release fertilizers in the agricultural soil	157:205	Durable chitosan-based coating material used as a barrier for slow-release fertilizers in the agricultural soil.
34920058	7	4	theme	Slow-release	908:919	arg1	efficiency					921:930	Slow-release efficiency	908:930	Slow-release efficiency	908:930	Slow-release efficiency was evaluated by determining the rate of phosphorus release from the covered granules into water and soil.
34920058	0	5	theme	NPK	79:81	arg1	fertilizer					83:92	slow release NPK fertilizer	66:92	slow release NPK fertilizer	66:92	Chitosan-kaolinite clay composite as durable coating material for slow release NPK fertilizer.
34920058	4	6	theme	clay	672:675	arg1	incorporation					677:689	the kaolinite clay incorporation	658:689	the kaolinite clay incorporation	658:689	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	3	7	theme	coating	357:363	arg1	material					365:372	the proposed coating material	344:372	the proposed coating material	344:372	In present paper, the proposed coating material was prepared on the basis of chitosan-kaolinite composite (CS-Gl-K).
34920058	4	8	theme	Electron	509:516	arg1	SEM					530:532	SEM	530:532	SEM	530:532	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	4	8	theme	Electron	509:516	arg1	Microscopy					518:527	Scanning Electron Microscopy	500:527	Scanning Electron Microscopy (SEM)	500:533	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	1	9	from	fertilizers	170:180	arg1	soil					202:205	the agricultural soil	185:205	the agricultural soil	185:205	Durable chitosan-based coating material used as a barrier for slow-release fertilizers in the agricultural soil.
34920058	9	10	theme	material	1216:1223	arg1	investigation					1185:1197	the biodegradation investigation	1166:1197	the biodegradation investigation of the composite material (CS-Gl-K) in soil	1166:1241	In addition, the biodegradation investigation of the composite material (CS-Gl-K) in soil was affirmed its durability.
34920058	4	11	dep	transform	451:459	arg1	used					625:628	used	625:628	were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties	620:717	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	11	12	theme	kaolinite-clay	1450:1463	arg1	pores					1465:1469	the kaolinite-clay pores	1446:1469	the kaolinite-clay pores	1446:1469	The FTIR, ATG and XRD spectra revealed a good intercalation between the kaolinite-clay pores and chitosan chains.
34920058	6	13	theme	NPK	801:803	arg1	fertilizer					813:822	The NPK mineral fertilizer	797:822	The NPK mineral fertilizer	797:822	The NPK mineral fertilizer was coated according to the dip-immersing process of chitosan-kaolinite composites.
34920058	4	14	theme	incorporation	677:689	arg1	influence					645:653	the influence	641:653	the influence of the kaolinite clay incorporation in chitosan film properties	641:717	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	10	15	theme	low	1336:1338	arg1	properties					1324:1333	good slow-release properties	1306:1333	good slow-release properties	1306:1333	The proposed coating material has good slow-release properties, low cost and is environmentally friendly.
34920058	10	15	theme	low	1336:1338	arg1	cost					1340:1343	low cost	1336:1343	low cost	1336:1343	The proposed coating material has good slow-release properties, low cost and is environmentally friendly.
34920058	11	16	theme	chitosan	1475:1482	arg1	chains					1484:1489	chitosan chains	1475:1489	chitosan chains	1475:1489	The FTIR, ATG and XRD spectra revealed a good intercalation between the kaolinite-clay pores and chitosan chains.
34920058	8	17	theme	NPK/CS-Gl-K	1080:1090	arg1	granules					1092:1099	coated NPK/CS-Gl-K granules	1073:1099	coated NPK/CS-Gl-K granules	1073:1099	Moreover, phosphorus release from coated NPK/CS-Gl-K granules was generally delayed contrasted with NPK/uncoated.
34920058	7	18	theme	release	984:990	arg1	rate					965:968	the rate	961:968	the rate of phosphorus release from the covered granules into water and soil	961:1036	Slow-release efficiency was evaluated by determining the rate of phosphorus release from the covered granules into water and soil.
34920058	4	19	from	influence	645:653	arg1	properties					708:717	chitosan film properties	694:717	chitosan film properties	694:717	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	3	20	theme	proposed	348:355	arg1	material					365:372	the proposed coating material	344:372	the proposed coating material	344:372	In present paper, the proposed coating material was prepared on the basis of chitosan-kaolinite composite (CS-Gl-K).
34920058	6	21	theme	composites	896:905	arg1	process					866:872	the dip-immersing process	848:872	the dip-immersing process of chitosan-kaolinite composites	848:905	The NPK mineral fertilizer was coated according to the dip-immersing process of chitosan-kaolinite composites.
34920058	0	22	theme	coating	45:51	arg1	material					53:60	durable coating material	37:60	durable coating material for slow release NPK fertilizer	37:92	Chitosan-kaolinite clay composite as durable coating material for slow release NPK fertilizer.
34920058	11	23	theme	FTIR	1382:1385	arg1	spectra					1400:1406	The FTIR, ATG and XRD spectra	1378:1406	spectra	1400:1406	The FTIR, ATG and XRD spectra revealed a good intercalation between the kaolinite-clay pores and chitosan chains.
34920058	7	24	from	granules	1009:1016	arg1	rate					965:968	the rate	961:968	the rate of phosphorus release from the covered granules into water and soil	961:1036	Slow-release efficiency was evaluated by determining the rate of phosphorus release from the covered granules into water and soil.
34920058	7	24	from	granules	1009:1016	arg1	release					984:990	phosphorus release	973:990	phosphorus release from the covered granules into water and soil	973:1036	Slow-release efficiency was evaluated by determining the rate of phosphorus release from the covered granules into water and soil.
34920058	7	25	theme	phosphorus	973:982	arg1	release					984:990	phosphorus release	973:990	phosphorus release from the covered granules into water and soil	973:1036	Slow-release efficiency was evaluated by determining the rate of phosphorus release from the covered granules into water and soil.
34920058	3	26	theme	present	329:335	arg1	paper					337:341	present paper	329:341	present paper	329:341	In present paper, the proposed coating material was prepared on the basis of chitosan-kaolinite composite (CS-Gl-K).
34920058	10	27	theme	slow-release	1311:1322	arg1	properties					1324:1333	good slow-release properties	1306:1333	good slow-release properties	1306:1333	The proposed coating material has good slow-release properties, low cost and is environmentally friendly.
34920058	10	27	theme	slow-release	1311:1322	arg1	cost					1340:1343	low cost	1336:1343	low cost	1336:1343	The proposed coating material has good slow-release properties, low cost and is environmentally friendly.
34920058	1	28	theme	agricultural	189:200	arg1	soil					202:205	the agricultural soil	185:205	the agricultural soil	185:205	Durable chitosan-based coating material used as a barrier for slow-release fertilizers in the agricultural soil.
34920058	0	29	theme	Chitosan-kaolinite	0:17	arg1	clay					19:22	Chitosan-kaolinite clay	0:22	Chitosan-kaolinite clay composite as durable coating material for slow release NPK fertilizer.	0:93	Chitosan-kaolinite clay composite as durable coating material for slow release NPK fertilizer.
34920058	6	30	theme	mineral	805:811	arg1	fertilizer					813:822	The NPK mineral fertilizer	797:822	The NPK mineral fertilizer	797:822	The NPK mineral fertilizer was coated according to the dip-immersing process of chitosan-kaolinite composites.
34920058	11	31	theme	good	1419:1422	arg1	intercalation					1424:1436	a good intercalation	1417:1436	a good intercalation between the kaolinite-clay pores and chitosan chains	1417:1489	The FTIR, ATG and XRD spectra revealed a good intercalation between the kaolinite-clay pores and chitosan chains.
34920058	1	32	theme	Durable	95:101	arg1	material					126:133	Durable chitosan-based coating material	95:133	Durable chitosan-based coating material	95:133	Durable chitosan-based coating material used as a barrier for slow-release fertilizers in the agricultural soil.
34920058	10	33	theme	environmentally	1352:1366	arg1	friendly					1368:1375	environmentally friendly	1352:1375	environmentally friendly	1352:1375	The proposed coating material has good slow-release properties, low cost and is environmentally friendly.
34920058	4	34	theme	thermogravimetric	536:552	arg1	analysis					554:561	thermogravimetric analysis	536:561	thermogravimetric analysis (ATG)	536:567	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	4	34	theme	thermogravimetric	536:552	arg1	ATG					564:566	ATG	564:566	ATG	564:566	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	1	35	theme	chitosan-based	103:116	arg1	material					126:133	Durable chitosan-based coating material	95:133	Durable chitosan-based coating material	95:133	Durable chitosan-based coating material used as a barrier for slow-release fertilizers in the agricultural soil.
34920058	1	36	theme	coating	118:124	arg1	material					126:133	Durable chitosan-based coating material	95:133	Durable chitosan-based coating material	95:133	Durable chitosan-based coating material used as a barrier for slow-release fertilizers in the agricultural soil.
34920058	9	37	from	investigation	1185:1197	arg1	soil					1238:1241	soil	1238:1241	soil	1238:1241	In addition, the biodegradation investigation of the composite material (CS-Gl-K) in soil was affirmed its durability.
34920058	3	38	theme	chitosan-kaolinite	403:420	arg1	CS-Gl-K					433:439	CS-Gl-K	433:439	CS-Gl-K	433:439	In present paper, the proposed coating material was prepared on the basis of chitosan-kaolinite composite (CS-Gl-K).
34920058	3	38	theme	chitosan-kaolinite	403:420	arg1	composite					422:430	chitosan-kaolinite composite	403:430	chitosan-kaolinite composite (CS-Gl-K)	403:440	In present paper, the proposed coating material was prepared on the basis of chitosan-kaolinite composite (CS-Gl-K).
34920058	0	39	theme	durable	37:43	arg1	material					53:60	durable coating material	37:60	durable coating material for slow release NPK fertilizer	37:92	Chitosan-kaolinite clay composite as durable coating material for slow release NPK fertilizer.
34920058	3	40	theme	composite	422:430	arg1	basis					394:398	the basis	390:398	the basis of chitosan-kaolinite composite (CS-Gl-K)	390:440	In present paper, the proposed coating material was prepared on the basis of chitosan-kaolinite composite (CS-Gl-K).
34920058	6	41	theme	dip-immersing	852:864	arg1	process					866:872	the dip-immersing process	848:872	the dip-immersing process of chitosan-kaolinite composites	848:905	The NPK mineral fertilizer was coated according to the dip-immersing process of chitosan-kaolinite composites.
34920058	8	42	dep	delayed	1115:1121	arg1	contrasted					1123:1132	contrasted	1123:1132	contrasted with NPK/uncoated	1123:1150	Moreover, phosphorus release from coated NPK/CS-Gl-K granules was generally delayed contrasted with NPK/uncoated.
34920058	8	43	from	granules	1092:1099	arg1	release					1060:1066	phosphorus release	1049:1066	phosphorus release from coated NPK/CS-Gl-K granules	1049:1099	Moreover, phosphorus release from coated NPK/CS-Gl-K granules was generally delayed contrasted with NPK/uncoated.
34920058	4	44	theme	infrared	461:468	arg1	spectroscopy					470:481	infrared spectroscopy	461:481	infrared spectroscopy	461:481	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	2	45	theme	intense	236:242	arg1	usage					244:248	the intense usage	232:248	the intense usage of fertilizer	232:262	This approach decreases the intense usage of fertilizer and works on their accessibility for the plants' necessities.
34920058	4	46	theme	chitosan	694:701	arg1	properties					708:717	chitosan film properties	694:717	chitosan film properties	694:717	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	11	47	theme	ATG	1388:1390	arg1	spectra					1400:1406	The FTIR, ATG and XRD spectra	1378:1406	spectra	1400:1406	The FTIR, ATG and XRD spectra revealed a good intercalation between the kaolinite-clay pores and chitosan chains.
34920058	8	48	theme	coated	1073:1078	arg1	granules					1092:1099	coated NPK/CS-Gl-K granules	1073:1099	coated NPK/CS-Gl-K granules	1073:1099	Moreover, phosphorus release from coated NPK/CS-Gl-K granules was generally delayed contrasted with NPK/uncoated.
34920058	9	49	theme	biodegradation	1170:1183	arg1	investigation					1185:1197	the biodegradation investigation	1166:1197	the biodegradation investigation of the composite material (CS-Gl-K) in soil	1166:1241	In addition, the biodegradation investigation of the composite material (CS-Gl-K) in soil was affirmed its durability.
34920058	11	50	theme	XRD	1396:1398	arg1	spectra					1400:1406	The FTIR, ATG and XRD spectra	1378:1406	spectra	1400:1406	The FTIR, ATG and XRD spectra revealed a good intercalation between the kaolinite-clay pores and chitosan chains.
34920058	10	51	theme	proposed	1276:1283	arg1	material					1293:1300	The proposed coating material	1272:1300	The proposed coating material	1272:1300	The proposed coating material has good slow-release properties, low cost and is environmentally friendly.
34920058	5	52	theme	chitosan	748:755	arg1	composites					757:766	the chitosan composites	744:766	the chitosan composites	744:766	The characterization of the chitosan composites has been thoroughly studied.
34920058	7	53	from	rate	965:968	arg1	granules					1009:1016	the covered granules	997:1016	the covered granules	997:1016	Slow-release efficiency was evaluated by determining the rate of phosphorus release from the covered granules into water and soil.
34920058	1	54	used	used	135:138	arg2	material					126:133	Durable chitosan-based coating material	95:133	Durable chitosan-based coating material	95:133	Durable chitosan-based coating material used as a barrier for slow-release fertilizers in the agricultural soil.
34920058	10	55	contain	has	1302:1304	arg1	material					1293:1300	The proposed coating material	1272:1300	The proposed coating material	1272:1300	The proposed coating material has good slow-release properties, low cost and is environmentally friendly.
34920058	10	55	contain	has	1302:1304	arg2	cost					1340:1343	low cost	1336:1343	low cost	1336:1343	The proposed coating material has good slow-release properties, low cost and is environmentally friendly.
34920058	10	55	contain	has	1302:1304	arg2	properties					1324:1333	good slow-release properties	1306:1333	good slow-release properties	1306:1333	The proposed coating material has good slow-release properties, low cost and is environmentally friendly.
34920058	9	56	theme	composite	1206:1214	arg1	CS-Gl-K					1226:1232	CS-Gl-K	1226:1232	CS-Gl-K	1226:1232	In addition, the biodegradation investigation of the composite material (CS-Gl-K) in soil was affirmed its durability.
34920058	9	56	theme	composite	1206:1214	arg1	material					1216:1223	the composite material	1202:1223	the composite material (CS-Gl-K)	1202:1233	In addition, the biodegradation investigation of the composite material (CS-Gl-K) in soil was affirmed its durability.
34920058	4	57	used	used	625:628	arg2	analysis					483:490	analysis	483:490	analysis (FTIR)	483:497	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	4	57	used	used	625:628	arg2	SEM					530:532	SEM	530:532	SEM	530:532	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	4	57	used	used	625:628	arg2	FTIR					493:496	FTIR	493:496	FTIR	493:496	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	4	57	used	used	625:628	arg2	Microscopy					518:527	Scanning Electron Microscopy	500:527	Scanning Electron Microscopy (SEM)	500:533	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	4	57	used	used	625:628	arg2	analysis					554:561	thermogravimetric analysis	536:561	thermogravimetric analysis (ATG)	536:567	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	4	57	used	used	625:628	arg2	XRD					570:572	XRD	570:572	XRD	570:572	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	4	57	used	used	625:628	arg2	ATG					564:566	ATG	564:566	ATG	564:566	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	4	57	used	used	625:628	arg2	studies					612:618	biodegradability studies	595:618	biodegradability studies	595:618	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	4	57	used	used	625:628	arg2	degree					584:589	swelling degree	575:589	swelling degree	575:589	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	4	58	theme	film	703:706	arg1	properties					708:717	chitosan film properties	694:717	chitosan film properties	694:717	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	4	59	theme	swelling	575:582	arg1	degree					584:589	swelling degree	575:589	swelling degree	575:589	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	0	60	theme	release	71:77	arg1	fertilizer					83:92	slow release NPK fertilizer	66:92	slow release NPK fertilizer	66:92	Chitosan-kaolinite clay composite as durable coating material for slow release NPK fertilizer.
34920058	10	61	theme	coating	1285:1291	arg1	material					1293:1300	The proposed coating material	1272:1300	The proposed coating material	1272:1300	The proposed coating material has good slow-release properties, low cost and is environmentally friendly.
34920058	4	62	theme	kaolinite	662:670	arg1	incorporation					677:689	the kaolinite clay incorporation	658:689	the kaolinite clay incorporation	658:689	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	4	63	theme	biodegradability	595:610	arg1	studies					612:618	biodegradability studies	595:618	biodegradability studies	595:618	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	0	64	theme	slow	66:69	arg1	fertilizer					83:92	slow release NPK fertilizer	66:92	slow release NPK fertilizer	66:92	Chitosan-kaolinite clay composite as durable coating material for slow release NPK fertilizer.
34920058	4	65	theme	Scanning	500:507	arg1	SEM					530:532	SEM	530:532	SEM	530:532	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	4	65	theme	Scanning	500:507	arg1	Microscopy					518:527	Scanning Electron Microscopy	500:527	Scanning Electron Microscopy (SEM)	500:533	Fourier transform infrared spectroscopy analysis (FTIR), Scanning Electron Microscopy (SEM), thermogravimetric analysis (ATG), XRD, swelling degree and biodegradability studies were used to analyze the influence of the kaolinite clay incorporation in chitosan film properties.
34920058	2	66	theme	fertilizer	253:262	arg1	usage					244:248	the intense usage	232:248	the intense usage of fertilizer	232:262	This approach decreases the intense usage of fertilizer and works on their accessibility for the plants' necessities.
34920058	7	67	theme	covered	1001:1007	arg1	granules					1009:1016	the covered granules	997:1016	the covered granules	997:1016	Slow-release efficiency was evaluated by determining the rate of phosphorus release from the covered granules into water and soil.
34920058	10	68	theme	good	1306:1309	arg1	properties					1324:1333	good slow-release properties	1306:1333	good slow-release properties	1306:1333	The proposed coating material has good slow-release properties, low cost and is environmentally friendly.
34920058	10	68	theme	good	1306:1309	arg1	cost					1340:1343	low cost	1336:1343	low cost	1336:1343	The proposed coating material has good slow-release properties, low cost and is environmentally friendly.
32114168	3	0	theme	pH	635:636	arg1	range					621:625	the range	617:625	the range of 7-10 pH	617:636	The maximum sorption (qe, 7.55-24.57 mg/g) of 2,4-DCP was achieved in the range of 7-10 pH, 0.05 g composite dose, 25 mg/L initial concentration of 2,4-DCP and 120 min contact time at 30 °C.
32114168	1	1	with	biocomposites	188:200	arg1	BH					308:309	BH	308:309	BH	308:309	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	1	1	with	biocomposites	188:200	arg1	husk					302:305	cellulosic biomass barley husk	276:305	cellulosic biomass barley husk (BH)	276:310	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	2	2	dep	native	449:454	arg1	PPY/BH					475:480	PPY/BH	475:480	PPY/BH	475:480	The sorption of 2,4-DCP was studied using native and biocomposites (PPY/BH, PANI/BH and NaAlg/BH) as function of various process variables.
32114168	2	2	dep	native	449:454	arg1	NaAlg/BH					495:502	NaAlg/BH	495:502	NaAlg/BH	495:502	The sorption of 2,4-DCP was studied using native and biocomposites (PPY/BH, PANI/BH and NaAlg/BH) as function of various process variables.
32114168	2	2	dep	native	449:454	arg1	PANI/BH					483:489	PANI/BH	483:489	PANI/BH	483:489	The sorption of 2,4-DCP was studied using native and biocomposites (PPY/BH, PANI/BH and NaAlg/BH) as function of various process variables.
32114168	7	3	theme	salts	1135:1139	arg1	Presence					1123:1130	Presence	1123:1130	Presence of salts	1123:1139	Presence of salts affected the 2,4-DCP adsorption negatively.
32114168	4	4	theme	amino	784:788	arg1	hydroxyl					791:798	amino, hydroxyl and carboxylic groups	784:820	hydroxyl	791:798	The FTIR analysis revealed the involvement of amino, hydroxyl and carboxylic groups for the binding of 2,4-DCP on the surface of biocomposites.
32114168	1	5	theme	media	400:404	arg1	removal					347:353	the removal	343:353	the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media	343:404	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	4	6	theme	hydroxyl	791:798	arg1	groups					815:820	amino, hydroxyl and carboxylic groups	784:820	groups	815:820	The FTIR analysis revealed the involvement of amino, hydroxyl and carboxylic groups for the binding of 2,4-DCP on the surface of biocomposites.
32114168	1	7	theme	sodium	247:252	arg1	NaAlg					264:268	NaAlg	264:268	NaAlg	264:268	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	1	7	theme	sodium	247:252	arg1	alginate					254:261	sodium alginate	247:261	sodium alginate (NaAlg)	247:269	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	6	8	theme	exothermic	1082:1091	arg1	nature					1104:1109	the favorable and exothermic adsorption nature	1064:1109	the favorable and exothermic adsorption nature of 2,4-DCP	1064:1120	The ∆G, ∆H and ∆S parameters were also computed, which revealed the favorable and exothermic adsorption nature of 2,4-DCP.
32114168	1	9	theme	alginate	254:261	arg1	polyaniline					224:234	polyaniline	224:234	polyaniline (PANI)	224:241	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	1	9	theme	alginate	254:261	arg1	polypyrrole					205:215	polypyrrole	205:215	polypyrrole (PPY)	205:221	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	1	9	theme	alginate	254:261	arg1	alginate					254:261	sodium alginate	247:261	sodium alginate (NaAlg)	247:269	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	1	9	theme	alginate	254:261	arg1	biocomposites					188:200	The biocomposites	184:200	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH)	184:310	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	3	10	theme	composite	646:654	arg1	dose					656:659	composite dose	646:659	composite dose	646:659	The maximum sorption (qe, 7.55-24.57 mg/g) of 2,4-DCP was achieved in the range of 7-10 pH, 0.05 g composite dose, 25 mg/L initial concentration of 2,4-DCP and 120 min contact time at 30 °C.
32114168	2	11	theme	variables	536:544	arg1	function					508:515	function	508:515	function of various process variables	508:544	The sorption of 2,4-DCP was studied using native and biocomposites (PPY/BH, PANI/BH and NaAlg/BH) as function of various process variables.
32114168	8	12	dep	65.12	1265:1269	arg1	to					1262:1263	to	1262:1263	to	1262:1263	HCl found to be efficient desorbing agent for 2,4-DCP from composites and up to 65.12% was eluted using 0.5 N solution.
32114168	8	13	theme	efficient	1201:1209	arg1	agent					1221:1225	efficient desorbing agent	1201:1225	efficient desorbing agent for 2,4-DCP from composites	1201:1253	HCl found to be efficient desorbing agent for 2,4-DCP from composites and up to 65.12% was eluted using 0.5 N solution.
32114168	4	14	theme	biocomposites	867:879	arg1	surface					856:862	the surface	852:862	the surface of biocomposites	852:879	The FTIR analysis revealed the involvement of amino, hydroxyl and carboxylic groups for the binding of 2,4-DCP on the surface of biocomposites.
32114168	5	15	theme	Freundlich	886:895	arg1	models					930:935	The Freundlich and pseudo second order kinetics models	882:935	The Freundlich and pseudo second order kinetics models	882:935	The Freundlich and pseudo second order kinetics models best explained the 2,4-DCP adsorption on to the biocomposites.
32114168	9	16	theme	efficiency	1326:1335	arg1	view					1308:1311	view	1308:1311	view of promising efficiency	1308:1335	In view of promising efficiency, the biocomposites have potential to remove 2,4-DCP form industrial effluents.
32114168	9	17	theme	promising	1316:1324	arg1	efficiency					1326:1335	promising efficiency	1316:1335	promising efficiency	1316:1335	In view of promising efficiency, the biocomposites have potential to remove 2,4-DCP form industrial effluents.
32114168	3	18	theme	25 mg/L	662:668	arg1	concentration					678:690	25 mg/L initial concentration	662:690	25 mg/L initial concentration of 2,4-DCP	662:701	The maximum sorption (qe, 7.55-24.57 mg/g) of 2,4-DCP was achieved in the range of 7-10 pH, 0.05 g composite dose, 25 mg/L initial concentration of 2,4-DCP and 120 min contact time at 30 °C.
32114168	4	19	theme	carboxylic	804:813	arg1	groups					815:820	amino, hydroxyl and carboxylic groups	784:820	groups	815:820	The FTIR analysis revealed the involvement of amino, hydroxyl and carboxylic groups for the binding of 2,4-DCP on the surface of biocomposites.
32114168	3	20	theme	initial	670:676	arg1	concentration					678:690	25 mg/L initial concentration	662:690	25 mg/L initial concentration of 2,4-DCP	662:701	The maximum sorption (qe, 7.55-24.57 mg/g) of 2,4-DCP was achieved in the range of 7-10 pH, 0.05 g composite dose, 25 mg/L initial concentration of 2,4-DCP and 120 min contact time at 30 °C.
32114168	9	21	contain	have	1356:1359	arg2	potential					1361:1369	potential	1361:1369	potential to remove 2,4-DCP form industrial effluents	1361:1413	In view of promising efficiency, the biocomposites have potential to remove 2,4-DCP form industrial effluents.
32114168	9	21	contain	have	1356:1359	arg1	biocomposites					1342:1354	the biocomposites	1338:1354	the biocomposites	1338:1354	In view of promising efficiency, the biocomposites have potential to remove 2,4-DCP form industrial effluents.
32114168	5	22	theme	2,4-DCP	956:962	arg1	adsorption					964:973	the 2,4-DCP adsorption	952:973	the 2,4-DCP adsorption on to the biocomposites	952:997	The Freundlich and pseudo second order kinetics models best explained the 2,4-DCP adsorption on to the biocomposites.
32114168	1	23	theme	polypyrrole	205:215	arg1	polyaniline					224:234	polyaniline	224:234	polyaniline (PANI)	224:241	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	1	23	theme	polypyrrole	205:215	arg1	polypyrrole					205:215	polypyrrole	205:215	polypyrrole (PPY)	205:221	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	1	23	theme	polypyrrole	205:215	arg1	alginate					254:261	sodium alginate	247:261	sodium alginate (NaAlg)	247:269	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	1	23	theme	polypyrrole	205:215	arg1	biocomposites					188:200	The biocomposites	184:200	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH)	184:310	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	8	24	theme	0.5 N	1289:1293	arg1	solution					1295:1302	0.5 N solution	1289:1302	0.5 N solution	1289:1302	HCl found to be efficient desorbing agent for 2,4-DCP from composites and up to 65.12% was eluted using 0.5 N solution.
32114168	3	25	dep	sorption	559:566	arg1	7.55-24.57 mg/g					573:587	7.55-24.57 mg/g	573:587	7.55-24.57 mg/g	573:587	The maximum sorption (qe, 7.55-24.57 mg/g) of 2,4-DCP was achieved in the range of 7-10 pH, 0.05 g composite dose, 25 mg/L initial concentration of 2,4-DCP and 120 min contact time at 30 °C.
32114168	3	25	dep	sorption	559:566	arg1	qe					569:570	qe	569:570	qe	569:570	The maximum sorption (qe, 7.55-24.57 mg/g) of 2,4-DCP was achieved in the range of 7-10 pH, 0.05 g composite dose, 25 mg/L initial concentration of 2,4-DCP and 120 min contact time at 30 °C.
32114168	0	26	theme	polypyrrole	17:27	arg1	Biocomposites					0:12	Biocomposites	0:12	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.	0:182	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	26	theme	polypyrrole	17:27	arg1	kinetics					110:117	kinetics	110:117	kinetics	110:117	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	26	theme	polypyrrole	17:27	arg1	polypyrrole					17:27	polypyrrole	17:27	polypyrrole	17:27	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	26	theme	polypyrrole	17:27	arg1	polyaniline					30:40	polyaniline	30:40	polyaniline	30:40	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	26	theme	polypyrrole	17:27	arg1	Adsorption-desorption					87:107	Adsorption-desorption	87:107	Adsorption-desorption	87:107	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	26	theme	polypyrrole	17:27	arg1	alginate					53:60	sodium alginate	46:60	sodium alginate	46:60	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	27	theme	thermodynamic	123:135	arg1	studies					137:143	thermodynamic studies	123:143	thermodynamic studies	123:143	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	6	28	theme	∆G	1004:1005	arg1	parameters					1018:1027	The ∆G, ∆H and ∆S parameters	1000:1027	The ∆G, ∆H and ∆S parameters	1000:1027	The ∆G, ∆H and ∆S parameters were also computed, which revealed the favorable and exothermic adsorption nature of 2,4-DCP.
32114168	1	29	theme	cellulosic	276:285	arg1	BH					308:309	BH	308:309	BH	308:309	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	1	29	theme	cellulosic	276:285	arg1	husk					302:305	cellulosic biomass barley husk	276:305	cellulosic biomass barley husk (BH)	276:310	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	3	30	theme	2,4-DCP	593:599	arg1	sorption					559:566	The maximum sorption	547:566	The maximum sorption (qe, 7.55-24.57 mg/g) of 2,4-DCP	547:599	The maximum sorption (qe, 7.55-24.57 mg/g) of 2,4-DCP was achieved in the range of 7-10 pH, 0.05 g composite dose, 25 mg/L initial concentration of 2,4-DCP and 120 min contact time at 30 °C.
32114168	5	31	theme	kinetics	921:928	arg1	models					930:935	The Freundlich and pseudo second order kinetics models	882:935	The Freundlich and pseudo second order kinetics models	882:935	The Freundlich and pseudo second order kinetics models best explained the 2,4-DCP adsorption on to the biocomposites.
32114168	6	32	theme	adsorption	1093:1102	arg1	nature					1104:1109	the favorable and exothermic adsorption nature	1064:1109	the favorable and exothermic adsorption nature of 2,4-DCP	1064:1120	The ∆G, ∆H and ∆S parameters were also computed, which revealed the favorable and exothermic adsorption nature of 2,4-DCP.
32114168	0	33	theme	polyaniline	30:40	arg1	Biocomposites					0:12	Biocomposites	0:12	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.	0:182	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	33	theme	polyaniline	30:40	arg1	kinetics					110:117	kinetics	110:117	kinetics	110:117	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	33	theme	polyaniline	30:40	arg1	polypyrrole					17:27	polypyrrole	17:27	polypyrrole	17:27	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	33	theme	polyaniline	30:40	arg1	polyaniline					30:40	polyaniline	30:40	polyaniline	30:40	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	33	theme	polyaniline	30:40	arg1	Adsorption-desorption					87:107	Adsorption-desorption	87:107	Adsorption-desorption	87:107	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	33	theme	polyaniline	30:40	arg1	alginate					53:60	sodium alginate	46:60	sodium alginate	46:60	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	1	34	theme	biomass	287:293	arg1	BH					308:309	BH	308:309	BH	308:309	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	1	34	theme	biomass	287:293	arg1	husk					302:305	cellulosic biomass barley husk	276:305	cellulosic biomass barley husk (BH)	276:310	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	4	35	theme	2,4-DCP	841:847	arg1	binding					830:836	the binding	826:836	the binding of 2,4-DCP on the surface of biocomposites	826:879	The FTIR analysis revealed the involvement of amino, hydroxyl and carboxylic groups for the binding of 2,4-DCP on the surface of biocomposites.
32114168	1	36	theme	2,4-dichlorophenol	358:375	arg1	media					400:404	2,4-dichlorophenol (2,4-DCP) form aqueous media	358:404	2,4-dichlorophenol (2,4-DCP) form aqueous media	358:404	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	3	37	theme	maximum	551:557	arg1	sorption					559:566	The maximum sorption	547:566	The maximum sorption (qe, 7.55-24.57 mg/g) of 2,4-DCP	547:599	The maximum sorption (qe, 7.55-24.57 mg/g) of 2,4-DCP was achieved in the range of 7-10 pH, 0.05 g composite dose, 25 mg/L initial concentration of 2,4-DCP and 120 min contact time at 30 °C.
32114168	0	38	theme	alginate	53:60	arg1	Biocomposites					0:12	Biocomposites	0:12	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.	0:182	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	38	theme	alginate	53:60	arg1	kinetics					110:117	kinetics	110:117	kinetics	110:117	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	38	theme	alginate	53:60	arg1	polypyrrole					17:27	polypyrrole	17:27	polypyrrole	17:27	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	38	theme	alginate	53:60	arg1	polyaniline					30:40	polyaniline	30:40	polyaniline	30:40	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	38	theme	alginate	53:60	arg1	Adsorption-desorption					87:107	Adsorption-desorption	87:107	Adsorption-desorption	87:107	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	38	theme	alginate	53:60	arg1	alginate					53:60	sodium alginate	46:60	sodium alginate	46:60	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	1	39	theme	barley	295:300	arg1	BH					308:309	BH	308:309	BH	308:309	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	1	39	theme	barley	295:300	arg1	husk					302:305	cellulosic biomass barley husk	276:305	cellulosic biomass barley husk (BH)	276:310	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	2	40	theme	process	528:534	arg1	variables					536:544	various process variables	520:544	various process variables	520:544	The sorption of 2,4-DCP was studied using native and biocomposites (PPY/BH, PANI/BH and NaAlg/BH) as function of various process variables.
32114168	4	41	from	binding	830:836	arg1	surface					856:862	the surface	852:862	the surface of biocomposites	852:879	The FTIR analysis revealed the involvement of amino, hydroxyl and carboxylic groups for the binding of 2,4-DCP on the surface of biocomposites.
32114168	9	42	theme	2,4-DCP	1381:1387	arg1	effluents					1405:1413	2,4-DCP form industrial effluents	1381:1413	2,4-DCP form industrial effluents	1381:1413	In view of promising efficiency, the biocomposites have potential to remove 2,4-DCP form industrial effluents.
32114168	0	43	theme	sodium	46:51	arg1	alginate					53:60	sodium alginate	46:60	sodium alginate	46:60	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	4	44	theme	groups	815:820	arg1	involvement					769:779	the involvement	765:779	the involvement of amino, hydroxyl and carboxylic groups for the binding of 2,4-DCP on the surface of biocomposites	765:879	The FTIR analysis revealed the involvement of amino, hydroxyl and carboxylic groups for the binding of 2,4-DCP on the surface of biocomposites.
32114168	2	45	theme	various	520:526	arg1	variables					536:544	various process variables	520:544	various process variables	520:544	The sorption of 2,4-DCP was studied using native and biocomposites (PPY/BH, PANI/BH and NaAlg/BH) as function of various process variables.
32114168	1	46	theme	2,4-DCP	378:384	arg1	media					400:404	2,4-dichlorophenol (2,4-DCP) form aqueous media	358:404	2,4-dichlorophenol (2,4-DCP) form aqueous media	358:404	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	4	47	theme	FTIR	742:745	arg1	analysis					747:754	The FTIR analysis	738:754	The FTIR analysis	738:754	The FTIR analysis revealed the involvement of amino, hydroxyl and carboxylic groups for the binding of 2,4-DCP on the surface of biocomposites.
32114168	8	48	from	composites	1244:1253	arg1	2,4-DCP					1231:1237	2,4-DCP	1231:1237	2,4-DCP from composites	1231:1253	HCl found to be efficient desorbing agent for 2,4-DCP from composites and up to 65.12% was eluted using 0.5 N solution.
32114168	9	49	theme	form	1389:1392	arg1	effluents					1405:1413	2,4-DCP form industrial effluents	1381:1413	2,4-DCP form industrial effluents	1381:1413	In view of promising efficiency, the biocomposites have potential to remove 2,4-DCP form industrial effluents.
32114168	3	50	theme	2,4-DCP	695:701	arg1	concentration					678:690	25 mg/L initial concentration	662:690	25 mg/L initial concentration of 2,4-DCP	662:701	The maximum sorption (qe, 7.55-24.57 mg/g) of 2,4-DCP was achieved in the range of 7-10 pH, 0.05 g composite dose, 25 mg/L initial concentration of 2,4-DCP and 120 min contact time at 30 °C.
32114168	3	50	theme	2,4-DCP	695:701	arg1	time					723:726	120 min contact time	707:726	120 min contact time	707:726	The maximum sorption (qe, 7.55-24.57 mg/g) of 2,4-DCP was achieved in the range of 7-10 pH, 0.05 g composite dose, 25 mg/L initial concentration of 2,4-DCP and 120 min contact time at 30 °C.
32114168	3	50	theme	2,4-DCP	695:701	arg1	dose					656:659	composite dose	646:659	composite dose	646:659	The maximum sorption (qe, 7.55-24.57 mg/g) of 2,4-DCP was achieved in the range of 7-10 pH, 0.05 g composite dose, 25 mg/L initial concentration of 2,4-DCP and 120 min contact time at 30 °C.
32114168	6	51	theme	2,4-DCP	1114:1120	arg1	nature					1104:1109	the favorable and exothermic adsorption nature	1064:1109	the favorable and exothermic adsorption nature of 2,4-DCP	1064:1120	The ∆G, ∆H and ∆S parameters were also computed, which revealed the favorable and exothermic adsorption nature of 2,4-DCP.
32114168	5	52	theme	pseudo	901:906	arg1	kinetics					921:928	pseudo second order kinetics	901:928	pseudo second order kinetics	901:928	The Freundlich and pseudo second order kinetics models best explained the 2,4-DCP adsorption on to the biocomposites.
32114168	0	53	with	Biocomposites	0:12	arg1	biomass					78:84	cellulosic biomass	67:84	cellulosic biomass	67:84	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	8	54	theme	desorbing	1211:1219	arg1	agent					1221:1225	efficient desorbing agent	1201:1225	efficient desorbing agent for 2,4-DCP from composites	1201:1253	HCl found to be efficient desorbing agent for 2,4-DCP from composites and up to 65.12% was eluted using 0.5 N solution.
32114168	1	55	theme	polyaniline	224:234	arg1	polyaniline					224:234	polyaniline	224:234	polyaniline (PANI)	224:241	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	1	55	theme	polyaniline	224:234	arg1	polypyrrole					205:215	polypyrrole	205:215	polypyrrole (PPY)	205:221	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	1	55	theme	polyaniline	224:234	arg1	alginate					254:261	sodium alginate	247:261	sodium alginate (NaAlg)	247:269	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	1	55	theme	polyaniline	224:234	arg1	biocomposites					188:200	The biocomposites	184:200	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH)	184:310	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	0	56	theme	2,4-dichlorophenol	164:181	arg1	removal					153:159	the removal	149:159	the removal of 2,4-dichlorophenol	149:181	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	1	57	theme	form	387:390	arg1	media					400:404	2,4-dichlorophenol (2,4-DCP) form aqueous media	358:404	2,4-dichlorophenol (2,4-DCP) form aqueous media	358:404	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	5	58	theme	second	908:913	arg1	kinetics					921:928	pseudo second order kinetics	901:928	pseudo second order kinetics	901:928	The Freundlich and pseudo second order kinetics models best explained the 2,4-DCP adsorption on to the biocomposites.
32114168	6	59	theme	∆S	1015:1016	arg1	parameters					1018:1027	The ∆G, ∆H and ∆S parameters	1000:1027	The ∆G, ∆H and ∆S parameters	1000:1027	The ∆G, ∆H and ∆S parameters were also computed, which revealed the favorable and exothermic adsorption nature of 2,4-DCP.
32114168	9	60	theme	industrial	1394:1403	arg1	effluents					1405:1413	2,4-DCP form industrial effluents	1381:1413	2,4-DCP form industrial effluents	1381:1413	In view of promising efficiency, the biocomposites have potential to remove 2,4-DCP form industrial effluents.
32114168	0	61	theme	cellulosic	67:76	arg1	biomass					78:84	cellulosic biomass	67:84	cellulosic biomass	67:84	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	1	62	theme	aqueous	392:398	arg1	media					400:404	2,4-dichlorophenol (2,4-DCP) form aqueous media	358:404	2,4-dichlorophenol (2,4-DCP) form aqueous media	358:404	The biocomposites of polypyrrole (PPY), polyaniline (PANI) and sodium alginate (NaAlg) with cellulosic biomass barley husk (BH) were prepared and employed for the removal of 2,4-dichlorophenol (2,4-DCP) form aqueous media.
32114168	5	63	theme	order	915:919	arg1	kinetics					921:928	pseudo second order kinetics	901:928	pseudo second order kinetics	901:928	The Freundlich and pseudo second order kinetics models best explained the 2,4-DCP adsorption on to the biocomposites.
32114168	7	64	theme	2,4-DCP	1154:1160	arg1	adsorption					1162:1171	the 2,4-DCP adsorption	1150:1171	the 2,4-DCP adsorption	1150:1171	Presence of salts affected the 2,4-DCP adsorption negatively.
32114168	6	65	theme	favorable	1068:1076	arg1	nature					1104:1109	the favorable and exothermic adsorption nature	1064:1109	the favorable and exothermic adsorption nature of 2,4-DCP	1064:1120	The ∆G, ∆H and ∆S parameters were also computed, which revealed the favorable and exothermic adsorption nature of 2,4-DCP.
32114168	3	66	theme	contact	715:721	arg1	time					723:726	120 min contact time	707:726	120 min contact time	707:726	The maximum sorption (qe, 7.55-24.57 mg/g) of 2,4-DCP was achieved in the range of 7-10 pH, 0.05 g composite dose, 25 mg/L initial concentration of 2,4-DCP and 120 min contact time at 30 °C.
32114168	2	67	theme	2,4-DCP	423:429	arg1	sorption					411:418	The sorption	407:418	The sorption of 2,4-DCP	407:429	The sorption of 2,4-DCP was studied using native and biocomposites (PPY/BH, PANI/BH and NaAlg/BH) as function of various process variables.
32114168	0	68	dep	Biocomposites	0:12	arg1	Biocomposites					0:12	Biocomposites	0:12	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.	0:182	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	68	dep	Biocomposites	0:12	arg1	kinetics					110:117	kinetics	110:117	kinetics	110:117	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	68	dep	Biocomposites	0:12	arg1	polypyrrole					17:27	polypyrrole	17:27	polypyrrole	17:27	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	68	dep	Biocomposites	0:12	arg1	studies					137:143	thermodynamic studies	123:143	thermodynamic studies	123:143	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	68	dep	Biocomposites	0:12	arg1	polyaniline					30:40	polyaniline	30:40	polyaniline	30:40	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	68	dep	Biocomposites	0:12	arg1	Adsorption-desorption					87:107	Adsorption-desorption	87:107	Adsorption-desorption	87:107	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	0	68	dep	Biocomposites	0:12	arg1	alginate					53:60	sodium alginate	46:60	sodium alginate	46:60	Biocomposites of polypyrrole, polyaniline and sodium alginate with cellulosic biomass: Adsorption-desorption, kinetics and thermodynamic studies for the removal of 2,4-dichlorophenol.
32114168	6	69	theme	∆H	1008:1009	arg1	parameters					1018:1027	The ∆G, ∆H and ∆S parameters	1000:1027	The ∆G, ∆H and ∆S parameters	1000:1027	The ∆G, ∆H and ∆S parameters were also computed, which revealed the favorable and exothermic adsorption nature of 2,4-DCP.
32114168	3	70	theme	120 min	707:713	arg1	time					723:726	120 min contact time	707:726	120 min contact time	707:726	The maximum sorption (qe, 7.55-24.57 mg/g) of 2,4-DCP was achieved in the range of 7-10 pH, 0.05 g composite dose, 25 mg/L initial concentration of 2,4-DCP and 120 min contact time at 30 °C.
34655580	0	0	theme	composite	90:98	arg1	adhesives					100:108	walnut protein isolate-xanthan gum composite adhesives	55:108	walnut protein isolate-xanthan gum composite adhesives using walnut protein	55:129	Physicochemical, structural and adhesion properties of walnut protein isolate-xanthan gum composite adhesives using walnut protein modified by ethanol.
34655580	5	1	theme	structures	1157:1166	arg1	conversion					1129:1138	a conformation conversion	1114:1138	a conformation conversion of the secondary structures from β-sheet to α-helix	1114:1190	Furthermore, the treatment also facilitated a conformation conversion of the secondary structures from β-sheet to α-helix, promoting the full unfolding of protein molecules.
34655580	6	2	dep	flat	1376:1379	arg1	combined					1393:1400	combined	1393:1400	combined more closely with water molecules	1393:1434	The microstructure analysis showed after 40% ethanol treatment, the WNPI structure was uniform, the surface of WNPI-XG adhesive was flat and smooth, combined more closely with water molecules.
34655580	6	2	dep	flat	1376:1379	arg1	flat					1376:1379	flat	1376:1379	flat	1376:1379	The microstructure analysis showed after 40% ethanol treatment, the WNPI structure was uniform, the surface of WNPI-XG adhesive was flat and smooth, combined more closely with water molecules.
34655580	6	2	dep	flat	1376:1379	arg1	surface					1344:1350	the surface	1340:1350	the surface of WNPI-XG adhesive	1340:1370	The microstructure analysis showed after 40% ethanol treatment, the WNPI structure was uniform, the surface of WNPI-XG adhesive was flat and smooth, combined more closely with water molecules.
34655580	7	3	theme	ethanol	1467:1473	arg1	treatment					1475:1483	ethanol treatment	1467:1483	ethanol treatment	1467:1483	By analyzing the influence of ethanol treatment on adhesion of WNPI-XG, the research laid a theoretical foundation for protein modification, providing good technical references for its development and utilization.
34655580	1	4	theme	high-protein	166:177	arg1	adhesives					179:187	Low-sugar and high-protein adhesives	152:187	Low-sugar and high-protein adhesives	152:187	Low-sugar and high-protein adhesives have broad market application prospects, while natural plant proteins have confronted technical bottlenecks due to their poor adhesion.
34655580	7	5	theme	treatment	1475:1483	arg1	influence					1454:1462	the influence	1450:1462	the influence of ethanol treatment on adhesion of WNPI-XG	1450:1506	By analyzing the influence of ethanol treatment on adhesion of WNPI-XG, the research laid a theoretical foundation for protein modification, providing good technical references for its development and utilization.
34655580	1	6	theme	technical	275:283	arg1	bottlenecks					285:295	technical bottlenecks	275:295	technical bottlenecks	275:295	Low-sugar and high-protein adhesives have broad market application prospects, while natural plant proteins have confronted technical bottlenecks due to their poor adhesion.
34655580	0	7	theme	gum	86:88	arg1	adhesives					100:108	walnut protein isolate-xanthan gum composite adhesives	55:108	walnut protein isolate-xanthan gum composite adhesives using walnut protein	55:129	Physicochemical, structural and adhesion properties of walnut protein isolate-xanthan gum composite adhesives using walnut protein modified by ethanol.
34655580	4	8	from	changes	935:941	arg1	contents					946:953	contents	946:953	contents of reactive sulfhydryl groups	946:983	It also indicated appropriate concentration of ethanol (40%) didn't change the molecular weight of WNPI-XG, but greatly strengthened the fluorescence intensity, leading changes in contents of reactive sulfhydryl groups, electrostatic forces, hydrophobic interactions, hydrogen bonds and disulfide bonds.
34655580	6	9	theme	WNPI	1312:1315	arg1	uniform					1331:1337	uniform	1331:1337	uniform	1331:1337	The microstructure analysis showed after 40% ethanol treatment, the WNPI structure was uniform, the surface of WNPI-XG adhesive was flat and smooth, combined more closely with water molecules.
34655580	6	9	theme	WNPI	1312:1315	arg1	structure					1317:1325	the WNPI structure	1308:1325	the WNPI structure	1308:1325	The microstructure analysis showed after 40% ethanol treatment, the WNPI structure was uniform, the surface of WNPI-XG adhesive was flat and smooth, combined more closely with water molecules.
34655580	2	10	from	effects	344:350	arg1	properties					417:426	the adhesion properties	404:426	the adhesion properties of walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives	404:494	In this study, the effects of ethanol with different concentrations (0-80%) on the adhesion properties of walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives were investigated.
34655580	5	11	theme	molecules	1233:1241	arg1	unfolding					1212:1220	the full unfolding	1203:1220	the full unfolding of protein molecules	1203:1241	Furthermore, the treatment also facilitated a conformation conversion of the secondary structures from β-sheet to α-helix, promoting the full unfolding of protein molecules.
34655580	4	12	theme	reactive	958:965	arg1	groups					978:983	reactive sulfhydryl groups	958:983	reactive sulfhydryl groups	958:983	It also indicated appropriate concentration of ethanol (40%) didn't change the molecular weight of WNPI-XG, but greatly strengthened the fluorescence intensity, leading changes in contents of reactive sulfhydryl groups, electrostatic forces, hydrophobic interactions, hydrogen bonds and disulfide bonds.
34655580	5	13	theme	full	1207:1210	arg1	unfolding					1212:1220	the full unfolding	1203:1220	the full unfolding of protein molecules	1203:1241	Furthermore, the treatment also facilitated a conformation conversion of the secondary structures from β-sheet to α-helix, promoting the full unfolding of protein molecules.
34655580	3	14	theme	WNPI-XG	554:560	arg1	strength					542:549	the bonding strength	530:549	the bonding strength of WNPI-XG treated with 40% ethanol reached 12.55 MPa, the denaturation temperature and the surface hydrophobicity	530:664	Results showed the bonding strength of WNPI-XG treated with 40% ethanol reached 12.55 MPa, the denaturation temperature and the surface hydrophobicity increased to 87.91 and 185.07 respectively, displaying the best rheological and texture properties.
34655580	5	15	from	conversion	1129:1138	arg1	β-sheet					1173:1179	β-sheet	1173:1179	β-sheet	1173:1179	Furthermore, the treatment also facilitated a conformation conversion of the secondary structures from β-sheet to α-helix, promoting the full unfolding of protein molecules.
34655580	4	16	theme	sulfhydryl	967:976	arg1	groups					978:983	reactive sulfhydryl groups	958:983	reactive sulfhydryl groups	958:983	It also indicated appropriate concentration of ethanol (40%) didn't change the molecular weight of WNPI-XG, but greatly strengthened the fluorescence intensity, leading changes in contents of reactive sulfhydryl groups, electrostatic forces, hydrophobic interactions, hydrogen bonds and disulfide bonds.
34655580	0	17	theme	adhesives	100:108	arg1	properties					41:50	Physicochemical, structural and adhesion properties	0:50	Physicochemical, structural and adhesion properties of walnut protein isolate-xanthan gum composite adhesives using walnut protein	0:129	Physicochemical, structural and adhesion properties of walnut protein isolate-xanthan gum composite adhesives using walnut protein modified by ethanol.
34655580	5	18	theme	conformation	1116:1127	arg1	conversion					1129:1138	a conformation conversion	1114:1138	a conformation conversion of the secondary structures from β-sheet to α-helix	1114:1190	Furthermore, the treatment also facilitated a conformation conversion of the secondary structures from β-sheet to α-helix, promoting the full unfolding of protein molecules.
34655580	4	19	theme	appropriate	784:794	arg1	concentration					796:808	appropriate concentration	784:808	appropriate concentration of ethanol (40%)	784:825	It also indicated appropriate concentration of ethanol (40%) didn't change the molecular weight of WNPI-XG, but greatly strengthened the fluorescence intensity, leading changes in contents of reactive sulfhydryl groups, electrostatic forces, hydrophobic interactions, hydrogen bonds and disulfide bonds.
34655580	2	20	theme	different	368:376	arg1	concentrations					378:391	different concentrations	368:391	different concentrations (0-80%)	368:399	In this study, the effects of ethanol with different concentrations (0-80%) on the adhesion properties of walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives were investigated.
34655580	2	20	theme	different	368:376	arg1	%					398:398	0-80%	394:398	0-80%	394:398	In this study, the effects of ethanol with different concentrations (0-80%) on the adhesion properties of walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives were investigated.
34655580	4	21	theme	groups	978:983	arg1	contents					946:953	contents	946:953	contents of reactive sulfhydryl groups	946:983	It also indicated appropriate concentration of ethanol (40%) didn't change the molecular weight of WNPI-XG, but greatly strengthened the fluorescence intensity, leading changes in contents of reactive sulfhydryl groups, electrostatic forces, hydrophobic interactions, hydrogen bonds and disulfide bonds.
34655580	4	22	theme	ethanol	813:819	arg1	concentration					796:808	appropriate concentration	784:808	appropriate concentration of ethanol (40%)	784:825	It also indicated appropriate concentration of ethanol (40%) didn't change the molecular weight of WNPI-XG, but greatly strengthened the fluorescence intensity, leading changes in contents of reactive sulfhydryl groups, electrostatic forces, hydrophobic interactions, hydrogen bonds and disulfide bonds.
34655580	0	23	theme	walnut	116:121	arg1	protein					123:129	walnut protein	116:129	walnut protein	116:129	Physicochemical, structural and adhesion properties of walnut protein isolate-xanthan gum composite adhesives using walnut protein modified by ethanol.
34655580	4	24	theme	molecular	845:853	arg1	weight					855:860	the molecular weight	841:860	the molecular weight of WNPI-XG	841:871	It also indicated appropriate concentration of ethanol (40%) didn't change the molecular weight of WNPI-XG, but greatly strengthened the fluorescence intensity, leading changes in contents of reactive sulfhydryl groups, electrostatic forces, hydrophobic interactions, hydrogen bonds and disulfide bonds.
34655580	2	25	theme	isolate-xanthan	446:460	arg1	adhesives					486:494	walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives	431:494	walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives	431:494	In this study, the effects of ethanol with different concentrations (0-80%) on the adhesion properties of walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives were investigated.
34655580	1	26	theme	broad	194:198	arg1	prospects					219:227	broad market application prospects	194:227	broad market application prospects	194:227	Low-sugar and high-protein adhesives have broad market application prospects, while natural plant proteins have confronted technical bottlenecks due to their poor adhesion.
34655580	2	27	theme	gum	462:464	arg1	adhesives					486:494	walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives	431:494	walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives	431:494	In this study, the effects of ethanol with different concentrations (0-80%) on the adhesion properties of walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives were investigated.
34655580	2	28	theme	adhesives	486:494	arg1	properties					417:426	the adhesion properties	404:426	the adhesion properties of walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives	404:494	In this study, the effects of ethanol with different concentrations (0-80%) on the adhesion properties of walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives were investigated.
34655580	4	29	theme	WNPI-XG	865:871	arg1	weight					855:860	the molecular weight	841:860	the molecular weight of WNPI-XG	841:871	It also indicated appropriate concentration of ethanol (40%) didn't change the molecular weight of WNPI-XG, but greatly strengthened the fluorescence intensity, leading changes in contents of reactive sulfhydryl groups, electrostatic forces, hydrophobic interactions, hydrogen bonds and disulfide bonds.
34655580	4	30	theme	electrostatic	986:998	arg1	changes					935:941	changes	935:941	changes in contents of reactive sulfhydryl groups	935:983	It also indicated appropriate concentration of ethanol (40%) didn't change the molecular weight of WNPI-XG, but greatly strengthened the fluorescence intensity, leading changes in contents of reactive sulfhydryl groups, electrostatic forces, hydrophobic interactions, hydrogen bonds and disulfide bonds.
34655580	4	30	theme	electrostatic	986:998	arg1	forces					1000:1005	electrostatic forces	986:1005	electrostatic forces	986:1005	It also indicated appropriate concentration of ethanol (40%) didn't change the molecular weight of WNPI-XG, but greatly strengthened the fluorescence intensity, leading changes in contents of reactive sulfhydryl groups, electrostatic forces, hydrophobic interactions, hydrogen bonds and disulfide bonds.
34655580	3	31	theme	%	577:577	arg1	ethanol					579:585	40% ethanol	575:585	40% ethanol reached 12.55 MPa, the denaturation temperature and the surface hydrophobicity	575:664	Results showed the bonding strength of WNPI-XG treated with 40% ethanol reached 12.55 MPa, the denaturation temperature and the surface hydrophobicity increased to 87.91 and 185.07 respectively, displaying the best rheological and texture properties.
34655580	1	32	theme	market	200:205	arg1	prospects					219:227	broad market application prospects	194:227	broad market application prospects	194:227	Low-sugar and high-protein adhesives have broad market application prospects, while natural plant proteins have confronted technical bottlenecks due to their poor adhesion.
34655580	0	33	theme	Physicochemical	0:14	arg1	properties					41:50	Physicochemical, structural and adhesion properties	0:50	Physicochemical, structural and adhesion properties of walnut protein isolate-xanthan gum composite adhesives using walnut protein	0:129	Physicochemical, structural and adhesion properties of walnut protein isolate-xanthan gum composite adhesives using walnut protein modified by ethanol.
34655580	5	34	theme	protein	1225:1231	arg1	molecules					1233:1241	protein molecules	1225:1241	protein molecules	1225:1241	Furthermore, the treatment also facilitated a conformation conversion of the secondary structures from β-sheet to α-helix, promoting the full unfolding of protein molecules.
34655580	6	35	theme	40	1285:1286	arg1	%					1287:1287	%	1287:1287	%	1287:1287	The microstructure analysis showed after 40% ethanol treatment, the WNPI structure was uniform, the surface of WNPI-XG adhesive was flat and smooth, combined more closely with water molecules.
34655580	4	36	theme	hydrogen	1034:1041	arg1	changes					935:941	changes	935:941	changes in contents of reactive sulfhydryl groups	935:983	It also indicated appropriate concentration of ethanol (40%) didn't change the molecular weight of WNPI-XG, but greatly strengthened the fluorescence intensity, leading changes in contents of reactive sulfhydryl groups, electrostatic forces, hydrophobic interactions, hydrogen bonds and disulfide bonds.
34655580	4	36	theme	hydrogen	1034:1041	arg1	bonds					1043:1047	hydrogen bonds	1034:1047	hydrogen bonds	1034:1047	It also indicated appropriate concentration of ethanol (40%) didn't change the molecular weight of WNPI-XG, but greatly strengthened the fluorescence intensity, leading changes in contents of reactive sulfhydryl groups, electrostatic forces, hydrophobic interactions, hydrogen bonds and disulfide bonds.
34655580	3	37	dep	showed	523:528	arg1	increased					666:674	increased	666:674	increased	666:674	Results showed the bonding strength of WNPI-XG treated with 40% ethanol reached 12.55 MPa, the denaturation temperature and the surface hydrophobicity increased to 87.91 and 185.07 respectively, displaying the best rheological and texture properties.
34655580	1	38	theme	application	207:217	arg1	prospects					219:227	broad market application prospects	194:227	broad market application prospects	194:227	Low-sugar and high-protein adhesives have broad market application prospects, while natural plant proteins have confronted technical bottlenecks due to their poor adhesion.
34655580	2	39	theme	protein	438:444	arg1	adhesives					486:494	walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives	431:494	walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives	431:494	In this study, the effects of ethanol with different concentrations (0-80%) on the adhesion properties of walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives were investigated.
34655580	1	40	theme	poor	310:313	arg1	adhesion					315:322	their poor adhesion	304:322	their poor adhesion	304:322	Low-sugar and high-protein adhesives have broad market application prospects, while natural plant proteins have confronted technical bottlenecks due to their poor adhesion.
34655580	6	41	theme	adhesive	1363:1370	arg1	WNPI-XG					1355:1361	WNPI-XG adhesive	1355:1370	WNPI-XG adhesive	1355:1370	The microstructure analysis showed after 40% ethanol treatment, the WNPI structure was uniform, the surface of WNPI-XG adhesive was flat and smooth, combined more closely with water molecules.
34655580	6	42	theme	microstructure	1248:1261	arg1	analysis					1263:1270	The microstructure analysis	1244:1270	The microstructure analysis	1244:1270	The microstructure analysis showed after 40% ethanol treatment, the WNPI structure was uniform, the surface of WNPI-XG adhesive was flat and smooth, combined more closely with water molecules.
34655580	2	43	theme	walnut	431:436	arg1	adhesives					486:494	walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives	431:494	walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives	431:494	In this study, the effects of ethanol with different concentrations (0-80%) on the adhesion properties of walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives were investigated.
34655580	7	44	theme	good	1588:1591	arg1	references					1603:1612	good technical references	1588:1612	good technical references	1588:1612	By analyzing the influence of ethanol treatment on adhesion of WNPI-XG, the research laid a theoretical foundation for protein modification, providing good technical references for its development and utilization.
34655580	6	45	theme	WNPI-XG	1355:1361	arg1	combined					1393:1400	combined	1393:1400	combined more closely with water molecules	1393:1434	The microstructure analysis showed after 40% ethanol treatment, the WNPI structure was uniform, the surface of WNPI-XG adhesive was flat and smooth, combined more closely with water molecules.
34655580	6	45	theme	WNPI-XG	1355:1361	arg1	flat					1376:1379	flat	1376:1379	flat	1376:1379	The microstructure analysis showed after 40% ethanol treatment, the WNPI structure was uniform, the surface of WNPI-XG adhesive was flat and smooth, combined more closely with water molecules.
34655580	6	45	theme	WNPI-XG	1355:1361	arg1	surface					1344:1350	the surface	1340:1350	the surface of WNPI-XG adhesive	1340:1370	The microstructure analysis showed after 40% ethanol treatment, the WNPI structure was uniform, the surface of WNPI-XG adhesive was flat and smooth, combined more closely with water molecules.
34655580	5	46	from	β-sheet	1173:1179	arg1	structures					1157:1166	the secondary structures	1143:1166	the secondary structures from β-sheet	1143:1179	Furthermore, the treatment also facilitated a conformation conversion of the secondary structures from β-sheet to α-helix, promoting the full unfolding of protein molecules.
34655580	5	46	from	β-sheet	1173:1179	arg1	conversion					1129:1138	a conformation conversion	1114:1138	a conformation conversion of the secondary structures from β-sheet to α-helix	1114:1190	Furthermore, the treatment also facilitated a conformation conversion of the secondary structures from β-sheet to α-helix, promoting the full unfolding of protein molecules.
34655580	3	47	theme	40	575:576	arg1	%					577:577	%	577:577	%	577:577	Results showed the bonding strength of WNPI-XG treated with 40% ethanol reached 12.55 MPa, the denaturation temperature and the surface hydrophobicity increased to 87.91 and 185.07 respectively, displaying the best rheological and texture properties.
34655580	2	48	theme	WNPI-XG	467:473	arg1	adhesives					486:494	walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives	431:494	walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives	431:494	In this study, the effects of ethanol with different concentrations (0-80%) on the adhesion properties of walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives were investigated.
34655580	2	49	theme	composite	476:484	arg1	adhesives					486:494	walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives	431:494	walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives	431:494	In this study, the effects of ethanol with different concentrations (0-80%) on the adhesion properties of walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives were investigated.
34655580	7	50	theme	protein	1556:1562	arg1	modification					1564:1575	protein modification	1556:1575	protein modification	1556:1575	By analyzing the influence of ethanol treatment on adhesion of WNPI-XG, the research laid a theoretical foundation for protein modification, providing good technical references for its development and utilization.
34655580	2	51	theme	adhesion	408:415	arg1	properties					417:426	the adhesion properties	404:426	the adhesion properties of walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives	404:494	In this study, the effects of ethanol with different concentrations (0-80%) on the adhesion properties of walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives were investigated.
34655580	0	52	theme	adhesion	32:39	arg1	properties					41:50	Physicochemical, structural and adhesion properties	0:50	Physicochemical, structural and adhesion properties of walnut protein isolate-xanthan gum composite adhesives using walnut protein	0:129	Physicochemical, structural and adhesion properties of walnut protein isolate-xanthan gum composite adhesives using walnut protein modified by ethanol.
34655580	3	53	dep	rheological	730:740	arg1	properties					754:763	properties	754:763	properties	754:763	Results showed the bonding strength of WNPI-XG treated with 40% ethanol reached 12.55 MPa, the denaturation temperature and the surface hydrophobicity increased to 87.91 and 185.07 respectively, displaying the best rheological and texture properties.
34655580	4	54	theme	hydrophobic	1008:1018	arg1	changes					935:941	changes	935:941	changes in contents of reactive sulfhydryl groups	935:983	It also indicated appropriate concentration of ethanol (40%) didn't change the molecular weight of WNPI-XG, but greatly strengthened the fluorescence intensity, leading changes in contents of reactive sulfhydryl groups, electrostatic forces, hydrophobic interactions, hydrogen bonds and disulfide bonds.
34655580	4	54	theme	hydrophobic	1008:1018	arg1	interactions					1020:1031	hydrophobic interactions	1008:1031	hydrophobic interactions	1008:1031	It also indicated appropriate concentration of ethanol (40%) didn't change the molecular weight of WNPI-XG, but greatly strengthened the fluorescence intensity, leading changes in contents of reactive sulfhydryl groups, electrostatic forces, hydrophobic interactions, hydrogen bonds and disulfide bonds.
34655580	2	55	with	ethanol	355:361	arg1	concentrations					378:391	different concentrations	368:391	different concentrations (0-80%)	368:399	In this study, the effects of ethanol with different concentrations (0-80%) on the adhesion properties of walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives were investigated.
34655580	2	55	with	ethanol	355:361	arg1	%					398:398	0-80%	394:398	0-80%	394:398	In this study, the effects of ethanol with different concentrations (0-80%) on the adhesion properties of walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives were investigated.
34655580	1	56	theme	natural	236:242	arg1	proteins					250:257	natural plant proteins	236:257	natural plant proteins	236:257	Low-sugar and high-protein adhesives have broad market application prospects, while natural plant proteins have confronted technical bottlenecks due to their poor adhesion.
34655580	0	57	theme	walnut	55:60	arg1	adhesives					100:108	walnut protein isolate-xanthan gum composite adhesives	55:108	walnut protein isolate-xanthan gum composite adhesives using walnut protein	55:129	Physicochemical, structural and adhesion properties of walnut protein isolate-xanthan gum composite adhesives using walnut protein modified by ethanol.
34655580	4	58	theme	fluorescence	903:914	arg1	intensity					916:924	the fluorescence intensity	899:924	the fluorescence intensity	899:924	It also indicated appropriate concentration of ethanol (40%) didn't change the molecular weight of WNPI-XG, but greatly strengthened the fluorescence intensity, leading changes in contents of reactive sulfhydryl groups, electrostatic forces, hydrophobic interactions, hydrogen bonds and disulfide bonds.
34655580	1	59	contain	have	189:192	arg2	prospects					219:227	broad market application prospects	194:227	broad market application prospects	194:227	Low-sugar and high-protein adhesives have broad market application prospects, while natural plant proteins have confronted technical bottlenecks due to their poor adhesion.
34655580	1	59	contain	have	189:192	arg1	adhesives					179:187	Low-sugar and high-protein adhesives	152:187	Low-sugar and high-protein adhesives	152:187	Low-sugar and high-protein adhesives have broad market application prospects, while natural plant proteins have confronted technical bottlenecks due to their poor adhesion.
34655580	2	60	theme	ethanol	355:361	arg1	effects					344:350	the effects	340:350	the effects of ethanol with different concentrations (0-80%) on the adhesion properties of walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives	340:494	In this study, the effects of ethanol with different concentrations (0-80%) on the adhesion properties of walnut protein isolate-xanthan gum (WNPI-XG) composite adhesives were investigated.
34655580	6	61	theme	water	1420:1424	arg1	molecules					1426:1434	water molecules	1420:1434	water molecules	1420:1434	The microstructure analysis showed after 40% ethanol treatment, the WNPI structure was uniform, the surface of WNPI-XG adhesive was flat and smooth, combined more closely with water molecules.
34655580	7	62	theme	WNPI-XG	1500:1506	arg1	adhesion					1488:1495	adhesion	1488:1495	adhesion of WNPI-XG	1488:1506	By analyzing the influence of ethanol treatment on adhesion of WNPI-XG, the research laid a theoretical foundation for protein modification, providing good technical references for its development and utilization.
34655580	6	63	theme	ethanol	1289:1295	arg1	treatment					1297:1305	40% ethanol treatment	1285:1305	40% ethanol treatment	1285:1305	The microstructure analysis showed after 40% ethanol treatment, the WNPI structure was uniform, the surface of WNPI-XG adhesive was flat and smooth, combined more closely with water molecules.
34655580	3	64	theme	surface	643:649	arg1	hydrophobicity					651:664	the surface hydrophobicity	639:664	the surface hydrophobicity	639:664	Results showed the bonding strength of WNPI-XG treated with 40% ethanol reached 12.55 MPa, the denaturation temperature and the surface hydrophobicity increased to 87.91 and 185.07 respectively, displaying the best rheological and texture properties.
34655580	4	65	theme	disulfide	1053:1061	arg1	changes					935:941	changes	935:941	changes in contents of reactive sulfhydryl groups	935:983	It also indicated appropriate concentration of ethanol (40%) didn't change the molecular weight of WNPI-XG, but greatly strengthened the fluorescence intensity, leading changes in contents of reactive sulfhydryl groups, electrostatic forces, hydrophobic interactions, hydrogen bonds and disulfide bonds.
34655580	4	65	theme	disulfide	1053:1061	arg1	bonds					1063:1067	disulfide bonds	1053:1067	disulfide bonds	1053:1067	It also indicated appropriate concentration of ethanol (40%) didn't change the molecular weight of WNPI-XG, but greatly strengthened the fluorescence intensity, leading changes in contents of reactive sulfhydryl groups, electrostatic forces, hydrophobic interactions, hydrogen bonds and disulfide bonds.
34655580	6	66	theme	%	1287:1287	arg1	treatment					1297:1305	40% ethanol treatment	1285:1305	40% ethanol treatment	1285:1305	The microstructure analysis showed after 40% ethanol treatment, the WNPI structure was uniform, the surface of WNPI-XG adhesive was flat and smooth, combined more closely with water molecules.
34655580	1	67	theme	plant	244:248	arg1	proteins					250:257	natural plant proteins	236:257	natural plant proteins	236:257	Low-sugar and high-protein adhesives have broad market application prospects, while natural plant proteins have confronted technical bottlenecks due to their poor adhesion.
34655580	0	68	theme	isolate-xanthan	70:84	arg1	adhesives					100:108	walnut protein isolate-xanthan gum composite adhesives	55:108	walnut protein isolate-xanthan gum composite adhesives using walnut protein	55:129	Physicochemical, structural and adhesion properties of walnut protein isolate-xanthan gum composite adhesives using walnut protein modified by ethanol.
34655580	7	69	from	influence	1454:1462	arg1	adhesion					1488:1495	adhesion	1488:1495	adhesion of WNPI-XG	1488:1506	By analyzing the influence of ethanol treatment on adhesion of WNPI-XG, the research laid a theoretical foundation for protein modification, providing good technical references for its development and utilization.
34655580	3	70	theme	denaturation	610:621	arg1	temperature					623:633	the denaturation temperature	606:633	the denaturation temperature	606:633	Results showed the bonding strength of WNPI-XG treated with 40% ethanol reached 12.55 MPa, the denaturation temperature and the surface hydrophobicity increased to 87.91 and 185.07 respectively, displaying the best rheological and texture properties.
34655580	1	71	theme	Low-sugar	152:160	arg1	adhesives					179:187	Low-sugar and high-protein adhesives	152:187	Low-sugar and high-protein adhesives	152:187	Low-sugar and high-protein adhesives have broad market application prospects, while natural plant proteins have confronted technical bottlenecks due to their poor adhesion.
34655580	7	72	theme	technical	1593:1601	arg1	references					1603:1612	good technical references	1588:1612	good technical references	1588:1612	By analyzing the influence of ethanol treatment on adhesion of WNPI-XG, the research laid a theoretical foundation for protein modification, providing good technical references for its development and utilization.
34655580	0	73	theme	protein	62:68	arg1	adhesives					100:108	walnut protein isolate-xanthan gum composite adhesives	55:108	walnut protein isolate-xanthan gum composite adhesives using walnut protein	55:129	Physicochemical, structural and adhesion properties of walnut protein isolate-xanthan gum composite adhesives using walnut protein modified by ethanol.
34655580	5	74	theme	secondary	1147:1155	arg1	structures					1157:1166	the secondary structures	1143:1166	the secondary structures from β-sheet	1143:1179	Furthermore, the treatment also facilitated a conformation conversion of the secondary structures from β-sheet to α-helix, promoting the full unfolding of protein molecules.
34655580	0	75	theme	structural	17:26	arg1	properties					41:50	Physicochemical, structural and adhesion properties	0:50	Physicochemical, structural and adhesion properties of walnut protein isolate-xanthan gum composite adhesives using walnut protein	0:129	Physicochemical, structural and adhesion properties of walnut protein isolate-xanthan gum composite adhesives using walnut protein modified by ethanol.
34655580	7	76	theme	theoretical	1529:1539	arg1	foundation					1541:1550	a theoretical foundation	1527:1550	a theoretical foundation for protein modification	1527:1575	By analyzing the influence of ethanol treatment on adhesion of WNPI-XG, the research laid a theoretical foundation for protein modification, providing good technical references for its development and utilization.
34655580	3	77	theme	bonding	534:540	arg1	strength					542:549	the bonding strength	530:549	the bonding strength of WNPI-XG treated with 40% ethanol reached 12.55 MPa, the denaturation temperature and the surface hydrophobicity	530:664	Results showed the bonding strength of WNPI-XG treated with 40% ethanol reached 12.55 MPa, the denaturation temperature and the surface hydrophobicity increased to 87.91 and 185.07 respectively, displaying the best rheological and texture properties.
34687415	0	0	theme	TiO2	103:106	arg1	nanoparticles					108:120	TiO2 nanoparticles	103:120	TiO2 nanoparticles	103:120	Enhancement of photocatalytic and biological activities of chitosan/activated carbon incorporated with TiO2 nanoparticles.
34687415	9	1	theme	CS/AC/0.4	1197:1205	arg1	%					1206:1206	%	1206:1206	%	1206:1206	CS/AC/0.4%TiO2 NPs showed maximum efficacy against larvicidal activity and pupicidal of mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET.
34687415	4	2	theme	SEM	602:604	arg1	images					606:611	The SEM images	598:611	The SEM images	598:611	The SEM images revealed the porous structure of CS/AC and TiO2 nanoparticles were uniformly distributed in the CS/AC matrix.
34687415	1	3	theme	charcoal	169:176	arg1	composites					183:192	Novel and sustainable chitosan (CS)/activated charcoal (AC) composites	123:192	Novel and sustainable chitosan (CS)/activated charcoal (AC) composites	123:192	Novel and sustainable chitosan (CS)/activated charcoal (AC) composites were prepared by cross-linking with epichlorohydrin (ECH) to form a porous structure.
34687415	2	4	theme	larvicidal	431:440	arg1	activities					457:466	the photocatalytic, antibacterial, larvicidal, and pupicidal activities	396:466	the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens	396:528	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	9	5	theme	%	1206:1206	arg1	NPs					1212:1214	CS/AC/0.4%TiO2 NPs	1197:1214	CS/AC/0.4%TiO2 NPs	1197:1214	CS/AC/0.4%TiO2 NPs showed maximum efficacy against larvicidal activity and pupicidal of mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET.
34687415	9	6	theme	larval	1394:1399	arg1	instars					1401:1407	the first, second, and third larval instars	1365:1407	the first, second, and third larval instars	1365:1407	CS/AC/0.4%TiO2 NPs showed maximum efficacy against larvicidal activity and pupicidal of mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET.
34687415	6	7	theme	photocatalytic	885:898	arg1	performance					900:910	photocatalytic performance	885:910	photocatalytic performance	885:910	Supporting TiO2 on a CS/AC matrix resulted in a significant increase in photocatalytic performance.
34687415	9	8	theme	TiO2	1207:1210	arg1	NPs					1212:1214	CS/AC/0.4%TiO2 NPs	1197:1214	CS/AC/0.4%TiO2 NPs	1197:1214	CS/AC/0.4%TiO2 NPs showed maximum efficacy against larvicidal activity and pupicidal of mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET.
34687415	2	9	theme	titanium	290:297	arg1	concentrations					331:344	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations	280:344	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w)	280:372	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	2	9	theme	titanium	290:297	arg1	%					367:367	0, 0.2, 0.4, and 0.8% w/w	347:371	0, 0.2, 0.4, and 0.8% w/w	347:371	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	4	10	theme	CS/AC	646:650	arg1	structure					633:641	the porous structure	622:641	the porous structure of CS/AC	622:650	The SEM images revealed the porous structure of CS/AC and TiO2 nanoparticles were uniformly distributed in the CS/AC matrix.
34687415	2	11	theme	Culex	516:520	arg1	pipiens					522:528	the Culex pipiens	512:528	the Culex pipiens	512:528	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	2	12	theme	Different	280:288	arg1	concentrations					331:344	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations	280:344	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w)	280:372	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	2	12	theme	Different	280:288	arg1	%					367:367	0, 0.2, 0.4, and 0.8% w/w	347:371	0, 0.2, 0.4, and 0.8% w/w	347:371	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	1	13	theme	porous	262:267	arg1	structure					269:277	a porous structure	260:277	a porous structure	260:277	Novel and sustainable chitosan (CS)/activated charcoal (AC) composites were prepared by cross-linking with epichlorohydrin (ECH) to form a porous structure.
34687415	9	14	with	82.95 ± 2.99	1504:1515	arg1	dose					1534:1537	3.24 mg/cm2 dose	1522:1537	3.24 mg/cm2 dose	1522:1537	CS/AC/0.4%TiO2 NPs showed maximum efficacy against larvicidal activity and pupicidal of mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET.
34687415	5	15	theme	composites	801:810	arg1	behavior					785:792	the photocatalytic behavior	766:792	the photocatalytic behavior of the composites	766:810	The degradation of RB dye was used to test the photocatalytic behavior of the composites.
34687415	9	16	theme	repellent	1457:1465	arg1	activity					1467:1474	the repellent activity	1453:1474	the repellent activity	1453:1474	CS/AC/0.4%TiO2 NPs showed maximum efficacy against larvicidal activity and pupicidal of mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET.
34687415	5	17	used	used	753:756	arg2	degradation					727:737	The degradation	723:737	The degradation of RB dye	723:747	The degradation of RB dye was used to test the photocatalytic behavior of the composites.
34687415	9	18	theme	maximum	1223:1229	arg1	efficacy					1231:1238	maximum efficacy	1223:1238	maximum efficacy against larvicidal activity and pupicidal of mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET	1223:1562	CS/AC/0.4%TiO2 NPs showed maximum efficacy against larvicidal activity and pupicidal of mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET.
34687415	6	19	from	increase	873:880	arg1	performance					900:910	photocatalytic performance	885:910	photocatalytic performance	885:910	Supporting TiO2 on a CS/AC matrix resulted in a significant increase in photocatalytic performance.
34687415	2	20	theme	antibacterial	416:428	arg1	activities					457:466	the photocatalytic, antibacterial, larvicidal, and pupicidal activities	396:466	the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens	396:528	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	7	21	theme	growth	998:1003	arg1	inhibition					1005:1014	bacterial growth inhibition	988:1014	bacterial growth inhibition against B. subtilis, S. aureus, E. coli, and P. aeruginosa	988:1073	The antibacterial activities supported by CS/AC/TiO2 NPs were evaluated by bacterial growth inhibition against B. subtilis, S. aureus, E. coli, and P. aeruginosa.
34687415	2	22	theme	TiO2	321:324	arg1	concentrations					331:344	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations	280:344	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w)	280:372	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	2	22	theme	TiO2	321:324	arg1	%					367:367	0, 0.2, 0.4, and 0.8% w/w	347:371	0, 0.2, 0.4, and 0.8% w/w	347:371	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	9	23	theme	first	1369:1373	arg1	instars					1401:1407	the first, second, and third larval instars	1365:1407	the first, second, and third larval instars	1365:1407	CS/AC/0.4%TiO2 NPs showed maximum efficacy against larvicidal activity and pupicidal of mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET.
34687415	6	24	theme	significant	861:871	arg1	increase					873:880	a significant increase	859:880	a significant increase in photocatalytic performance	859:910	Supporting TiO2 on a CS/AC matrix resulted in a significant increase in photocatalytic performance.
34687415	8	25	theme	inhibitory	1132:1141	arg1	effect					1143:1148	an inhibitory effect	1129:1148	an inhibitory effect	1129:1148	The results showed that CS/AC/TiO2 NPs composite has an inhibitory effect and therefore considered antibacterial agents.
34687415	9	26	theme	high	1485:1488	arg1	protection					1490:1499	high protection	1485:1499	high protection	1485:1499	CS/AC/0.4%TiO2 NPs showed maximum efficacy against larvicidal activity and pupicidal of mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET.
34687415	9	27	theme	pupal	1430:1434	arg1	mortality					1436:1444	pupal mortality	1430:1444	pupal mortality	1430:1444	CS/AC/0.4%TiO2 NPs showed maximum efficacy against larvicidal activity and pupicidal of mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET.
34687415	9	28	theme	larvicidal	1248:1257	arg1	activity					1259:1266	larvicidal activity	1248:1266	larvicidal activity	1248:1266	CS/AC/0.4%TiO2 NPs showed maximum efficacy against larvicidal activity and pupicidal of mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET.
34687415	1	29	with	cross-linking	211:223	arg1	ECH					247:249	ECH	247:249	ECH	247:249	Novel and sustainable chitosan (CS)/activated charcoal (AC) composites were prepared by cross-linking with epichlorohydrin (ECH) to form a porous structure.
34687415	1	29	with	cross-linking	211:223	arg1	epichlorohydrin					230:244	epichlorohydrin	230:244	epichlorohydrin (ECH)	230:250	Novel and sustainable chitosan (CS)/activated charcoal (AC) composites were prepared by cross-linking with epichlorohydrin (ECH) to form a porous structure.
34687415	2	30	theme	nanoparticle	307:318	arg1	concentrations					331:344	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations	280:344	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w)	280:372	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	2	30	theme	nanoparticle	307:318	arg1	%					367:367	0, 0.2, 0.4, and 0.8% w/w	347:371	0, 0.2, 0.4, and 0.8% w/w	347:371	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	9	31	theme	second	1376:1381	arg1	instars					1401:1407	the first, second, and third larval instars	1365:1407	the first, second, and third larval instars	1365:1407	CS/AC/0.4%TiO2 NPs showed maximum efficacy against larvicidal activity and pupicidal of mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET.
34687415	0	32	theme	photocatalytic	15:28	arg1	activities					45:54	photocatalytic and biological activities	15:54	photocatalytic and biological activities of chitosan/activated carbon	15:83	Enhancement of photocatalytic and biological activities of chitosan/activated carbon incorporated with TiO2 nanoparticles.
34687415	7	33	theme	bacterial	988:996	arg1	inhibition					1005:1014	bacterial growth inhibition	988:1014	bacterial growth inhibition against B. subtilis, S. aureus, E. coli, and P. aeruginosa	988:1073	The antibacterial activities supported by CS/AC/TiO2 NPs were evaluated by bacterial growth inhibition against B. subtilis, S. aureus, E. coli, and P. aeruginosa.
34687415	2	34	theme	dioxide	299:305	arg1	concentrations					331:344	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations	280:344	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w)	280:372	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	2	34	theme	dioxide	299:305	arg1	%					367:367	0, 0.2, 0.4, and 0.8% w/w	347:371	0, 0.2, 0.4, and 0.8% w/w	347:371	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	5	35	theme	RB	742:743	arg1	dye					745:747	RB dye	742:747	RB dye	742:747	The degradation of RB dye was used to test the photocatalytic behavior of the composites.
34687415	0	36	theme	activities	45:54	arg1	Enhancement					0:10	Enhancement	0:10	Enhancement of photocatalytic and biological activities of chitosan/activated carbon	0:83	Enhancement of photocatalytic and biological activities of chitosan/activated carbon incorporated with TiO2 nanoparticles.
34687415	2	37	theme	Bengal	492:497	arg1	dye					504:506	Rose Bengal (RB) dye	487:506	Rose Bengal (RB) dye	487:506	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	5	38	theme	dye	745:747	arg1	degradation					727:737	The degradation	723:737	The degradation of RB dye	723:747	The degradation of RB dye was used to test the photocatalytic behavior of the composites.
34687415	2	39	theme	w/w	369:371	arg1	concentrations					331:344	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations	280:344	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w)	280:372	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	2	39	theme	w/w	369:371	arg1	%					367:367	0, 0.2, 0.4, and 0.8% w/w	347:371	0, 0.2, 0.4, and 0.8% w/w	347:371	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	0	40	theme	biological	34:43	arg1	activities					45:54	photocatalytic and biological activities	15:54	photocatalytic and biological activities of chitosan/activated carbon	15:83	Enhancement of photocatalytic and biological activities of chitosan/activated carbon incorporated with TiO2 nanoparticles.
34687415	2	41	theme	Rose	487:490	arg1	RB					500:501	RB	500:501	RB	500:501	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	2	41	theme	Rose	487:490	arg1	Bengal					492:497	Rose Bengal	487:497	Rose Bengal (RB) dye	487:506	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	4	42	theme	TiO2	656:659	arg1	nanoparticles					661:673	TiO2 nanoparticles	656:673	TiO2 nanoparticles	656:673	The SEM images revealed the porous structure of CS/AC and TiO2 nanoparticles were uniformly distributed in the CS/AC matrix.
34687415	7	43	theme	antibacterial	917:929	arg1	activities					931:940	The antibacterial activities	913:940	The antibacterial activities supported by CS/AC/TiO2 NPs	913:968	The antibacterial activities supported by CS/AC/TiO2 NPs were evaluated by bacterial growth inhibition against B. subtilis, S. aureus, E. coli, and P. aeruginosa.
34687415	8	44	theme	antibacterial	1175:1187	arg1	agents					1189:1194	antibacterial agents	1175:1194	antibacterial agents	1175:1194	The results showed that CS/AC/TiO2 NPs composite has an inhibitory effect and therefore considered antibacterial agents.
34687415	0	45	theme	chitosan/activated	59:76	arg1	carbon					78:83	chitosan/activated carbon	59:83	chitosan/activated carbon	59:83	Enhancement of photocatalytic and biological activities of chitosan/activated carbon incorporated with TiO2 nanoparticles.
34687415	6	46	theme	CS/AC	834:838	arg1	matrix					840:845	a CS/AC matrix	832:845	a CS/AC matrix	832:845	Supporting TiO2 on a CS/AC matrix resulted in a significant increase in photocatalytic performance.
34687415	7	47	theme	CS/AC/TiO2	955:964	arg1	NPs					966:968	CS/AC/TiO2 NPs	955:968	CS/AC/TiO2 NPs	955:968	The antibacterial activities supported by CS/AC/TiO2 NPs were evaluated by bacterial growth inhibition against B. subtilis, S. aureus, E. coli, and P. aeruginosa.
34687415	9	48	theme	3.24 mg/cm2	1522:1532	arg1	dose					1534:1537	3.24 mg/cm2 dose	1522:1537	3.24 mg/cm2 dose	1522:1537	CS/AC/0.4%TiO2 NPs showed maximum efficacy against larvicidal activity and pupicidal of mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET.
34687415	1	49	theme	sustainable	133:143	arg1	AC					179:180	AC	179:180	AC	179:180	Novel and sustainable chitosan (CS)/activated charcoal (AC) composites were prepared by cross-linking with epichlorohydrin (ECH) to form a porous structure.
34687415	1	49	theme	sustainable	133:143	arg1	charcoal					169:176	Novel and sustainable chitosan (CS)/activated charcoal	123:176	Novel and sustainable chitosan (CS)/activated charcoal (AC) composites	123:192	Novel and sustainable chitosan (CS)/activated charcoal (AC) composites were prepared by cross-linking with epichlorohydrin (ECH) to form a porous structure.
34687415	2	50	theme	photocatalytic	400:413	arg1	activities					457:466	the photocatalytic, antibacterial, larvicidal, and pupicidal activities	396:466	the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens	396:528	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	9	51	theme	mosquito	1285:1292	arg1	vector					1294:1299	mosquito vector	1285:1299	mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET	1285:1562	CS/AC/0.4%TiO2 NPs showed maximum efficacy against larvicidal activity and pupicidal of mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET.
34687415	9	52	theme	control	1551:1557	arg1	DEET					1559:1562	control DEET	1551:1562	control DEET	1551:1562	CS/AC/0.4%TiO2 NPs showed maximum efficacy against larvicidal activity and pupicidal of mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET.
34687415	8	53	theme	NPs	1111:1113	arg1	composite					1115:1123	CS/AC/TiO2 NPs composite	1100:1123	CS/AC/TiO2 NPs composite	1100:1123	The results showed that CS/AC/TiO2 NPs composite has an inhibitory effect and therefore considered antibacterial agents.
34687415	9	54	theme	vector	1294:1299	arg1	efficacy					1231:1238	maximum efficacy	1223:1238	maximum efficacy against larvicidal activity and pupicidal of mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET	1223:1562	CS/AC/0.4%TiO2 NPs showed maximum efficacy against larvicidal activity and pupicidal of mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET.
34687415	4	55	theme	porous	626:631	arg1	structure					633:641	the porous structure	622:641	the porous structure of CS/AC	622:650	The SEM images revealed the porous structure of CS/AC and TiO2 nanoparticles were uniformly distributed in the CS/AC matrix.
34687415	1	56	theme	Novel	123:127	arg1	AC					179:180	AC	179:180	AC	179:180	Novel and sustainable chitosan (CS)/activated charcoal (AC) composites were prepared by cross-linking with epichlorohydrin (ECH) to form a porous structure.
34687415	1	56	theme	Novel	123:127	arg1	charcoal					169:176	Novel and sustainable chitosan (CS)/activated charcoal	123:176	Novel and sustainable chitosan (CS)/activated charcoal (AC) composites	123:192	Novel and sustainable chitosan (CS)/activated charcoal (AC) composites were prepared by cross-linking with epichlorohydrin (ECH) to form a porous structure.
34687415	2	57	theme	NPs	326:328	arg1	concentrations					331:344	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations	280:344	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w)	280:372	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	2	57	theme	NPs	326:328	arg1	%					367:367	0, 0.2, 0.4, and 0.8% w/w	347:371	0, 0.2, 0.4, and 0.8% w/w	347:371	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	8	58	theme	CS/AC/TiO2	1100:1109	arg1	composite					1115:1123	CS/AC/TiO2 NPs composite	1100:1123	CS/AC/TiO2 NPs composite	1100:1123	The results showed that CS/AC/TiO2 NPs composite has an inhibitory effect and therefore considered antibacterial agents.
34687415	2	59	theme	pupicidal	447:455	arg1	activities					457:466	the photocatalytic, antibacterial, larvicidal, and pupicidal activities	396:466	the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens	396:528	Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens.
34687415	9	60	theme	third	1388:1392	arg1	instars					1401:1407	the first, second, and third larval instars	1365:1407	the first, second, and third larval instars	1365:1407	CS/AC/0.4%TiO2 NPs showed maximum efficacy against larvicidal activity and pupicidal of mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET.
34687415	4	61	theme	CS/AC	709:713	arg1	matrix					715:720	the CS/AC matrix	705:720	the CS/AC matrix	705:720	The SEM images revealed the porous structure of CS/AC and TiO2 nanoparticles were uniformly distributed in the CS/AC matrix.
34687415	5	62	theme	photocatalytic	770:783	arg1	behavior					785:792	the photocatalytic behavior	766:792	the photocatalytic behavior of the composites	766:810	The degradation of RB dye was used to test the photocatalytic behavior of the composites.
34687415	0	63	theme	carbon	78:83	arg1	activities					45:54	photocatalytic and biological activities	15:54	photocatalytic and biological activities of chitosan/activated carbon	15:83	Enhancement of photocatalytic and biological activities of chitosan/activated carbon incorporated with TiO2 nanoparticles.
34687415	1	64	theme	/activated	158:167	arg1	AC					179:180	AC	179:180	AC	179:180	Novel and sustainable chitosan (CS)/activated charcoal (AC) composites were prepared by cross-linking with epichlorohydrin (ECH) to form a porous structure.
34687415	1	64	theme	/activated	158:167	arg1	charcoal					169:176	Novel and sustainable chitosan (CS)/activated charcoal	123:176	Novel and sustainable chitosan (CS)/activated charcoal (AC) composites	123:192	Novel and sustainable chitosan (CS)/activated charcoal (AC) composites were prepared by cross-linking with epichlorohydrin (ECH) to form a porous structure.
35014435	9	0	theme	CNC	1583:1585	arg1	addition					1587:1594	the CNC addition	1579:1594	the CNC addition up to 3%	1579:1603	Our temperature sweep experiments show that the CNC addition up to 3% does not change the rapid thermal gelation of the F127 solutions; therefore, these composites are suitable for smart drug delivery systems.
35014435	6	1	theme	softening	1119:1127	arg1	effect					1129:1134	The softening effect	1115:1134	The softening effect	1115:1134	The softening effect results from the disruption of the close packed micelles by the rodlike CNCs.
35014435	10	2	theme	viscosity	1801:1809	arg1	transition					1811:1820	abrupt viscosity transition	1794:1820	abrupt viscosity transition	1794:1820	On the other hand, at higher CNC concentrations, abrupt viscosity transition is not observed, rather the composite gels smoothly thicken with temperature in contrast to thermal thinning of the aqueous neat CNC.
35014435	1	3	theme	human	289:293	arg1	temperature					300:310	human body temperature	289:310	human body temperature	289:310	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions have been widely investigated in smart biomaterial applications due to the proximity of its critical gel temperature to human body temperature.
35014435	11	4	used	used	1974:1977	arg2	materials					2032:2040	bioviscostatic materials	2017:2040	bioviscostatic materials	2017:2040	Thus, they can be used as smartly adaptive biolubricants and bioviscostatic materials.
35014435	11	4	used	used	1974:1977	arg2	biolubricants					1999:2011	smartly adaptive biolubricants	1982:2011	smartly adaptive biolubricants	1982:2011	Thus, they can be used as smartly adaptive biolubricants and bioviscostatic materials.
35014435	11	4	used	used	1974:1977	arg2	they					1962:1965	they	1962:1965	they	1962:1965	Thus, they can be used as smartly adaptive biolubricants and bioviscostatic materials.
35014435	1	5	theme	triblock	140:147	arg1	solutions					159:167	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions	97:167	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions	97:167	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions have been widely investigated in smart biomaterial applications due to the proximity of its critical gel temperature to human body temperature.
35014435	4	6	theme	triblock	881:888	arg1	copolymer					890:898	the triblock copolymer	877:898	the triblock copolymer	877:898	Our results revealed an unprecedented role of CNC network formation on micellization and gelation behavior of the triblock copolymer.
35014435	3	7	theme	responsive	741:750	arg1	F127					761:764	the temperature responsive Pluronic F127	725:764	the temperature responsive Pluronic F127	725:764	Herein, we investigate the phase behavior and thermo-rheological properties of the composite hydrogels containing cellulose nanocrystals (up to 5% by weight) and the temperature responsive Pluronic F127.
35014435	1	8	theme	biomaterial	208:218	arg1	applications					220:231	smart biomaterial applications	202:231	smart biomaterial applications due to the proximity of its critical gel temperature to human body temperature	202:310	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions have been widely investigated in smart biomaterial applications due to the proximity of its critical gel temperature to human body temperature.
35014435	9	9	dep	addition	1587:1594	arg1	%					1603:1603	up to 3%	1596:1603	the CNC addition up to 3%	1579:1603	Our temperature sweep experiments show that the CNC addition up to 3% does not change the rapid thermal gelation of the F127 solutions; therefore, these composites are suitable for smart drug delivery systems.
35014435	6	10	theme	micelles	1184:1191	arg1	disruption					1153:1162	the disruption	1149:1162	the disruption of the close packed micelles by the rodlike CNCs	1149:1211	The softening effect results from the disruption of the close packed micelles by the rodlike CNCs.
35014435	1	11	theme	due	233:235	arg1	applications					220:231	smart biomaterial applications	202:231	smart biomaterial applications due to the proximity of its critical gel temperature to human body temperature	202:310	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions have been widely investigated in smart biomaterial applications due to the proximity of its critical gel temperature to human body temperature.
35014435	6	12	theme	close	1171:1175	arg1	micelles					1184:1191	the close packed micelles	1167:1191	the close packed micelles	1167:1191	The softening effect results from the disruption of the close packed micelles by the rodlike CNCs.
35014435	10	13	theme	composite	1850:1858	arg1	gels					1860:1863	the composite gels	1846:1863	the composite gels	1846:1863	On the other hand, at higher CNC concentrations, abrupt viscosity transition is not observed, rather the composite gels smoothly thicken with temperature in contrast to thermal thinning of the aqueous neat CNC.
35014435	5	14	theme	Pluronic	1095:1102	arg1	gels					1109:1112	the neat Pluronic F127 gels	1086:1112	the neat Pluronic F127 gels	1086:1112	Linear and nonlinear rheological analysis suggest that at low and moderate nanocrystal loadings (1-3% by weight), the composite gel remarkably becomes softer and deformable compared to the neat Pluronic F127 gels.
35014435	3	15	theme	phase	590:594	arg1	behavior					596:603	phase behavior	590:603	phase behavior	590:603	Herein, we investigate the phase behavior and thermo-rheological properties of the composite hydrogels containing cellulose nanocrystals (up to 5% by weight) and the temperature responsive Pluronic F127.
35014435	9	16	theme	rapid	1625:1629	arg1	gelation					1639:1646	the rapid thermal gelation	1621:1646	the rapid thermal gelation of the F127 solutions	1621:1668	Our temperature sweep experiments show that the CNC addition up to 3% does not change the rapid thermal gelation of the F127 solutions; therefore, these composites are suitable for smart drug delivery systems.
35014435	8	17	theme	nanocrystal	1418:1428	arg1	loading					1430:1436	4 to 5% nanocrystal loading	1410:1436	4 to 5% nanocrystal loading	1410:1436	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	1	18	theme	copolymer	149:157	arg1	solutions					159:167	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions	97:167	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions	97:167	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions have been widely investigated in smart biomaterial applications due to the proximity of its critical gel temperature to human body temperature.
35014435	3	19	theme	cellulose	677:685	arg1	nanocrystals					687:698	cellulose nanocrystals	677:698	cellulose nanocrystals (up to 5% by weight)	677:719	Herein, we investigate the phase behavior and thermo-rheological properties of the composite hydrogels containing cellulose nanocrystals (up to 5% by weight) and the temperature responsive Pluronic F127.
35014435	5	20	theme	nanocrystal	976:986	arg1	loadings					988:995	low and moderate nanocrystal loadings	959:995	low and moderate nanocrystal loadings (1-3% by weight)	959:1012	Linear and nonlinear rheological analysis suggest that at low and moderate nanocrystal loadings (1-3% by weight), the composite gel remarkably becomes softer and deformable compared to the neat Pluronic F127 gels.
35014435	9	21	theme	solutions	1660:1668	arg1	gelation					1639:1646	the rapid thermal gelation	1621:1646	the rapid thermal gelation of the F127 solutions	1621:1668	Our temperature sweep experiments show that the CNC addition up to 3% does not change the rapid thermal gelation of the F127 solutions; therefore, these composites are suitable for smart drug delivery systems.
35014435	3	22	theme	hydrogels	656:664	arg1	F127					761:764	the temperature responsive Pluronic F127	725:764	the temperature responsive Pluronic F127	725:764	Herein, we investigate the phase behavior and thermo-rheological properties of the composite hydrogels containing cellulose nanocrystals (up to 5% by weight) and the temperature responsive Pluronic F127.
35014435	3	22	theme	hydrogels	656:664	arg1	behavior					596:603	phase behavior	590:603	phase behavior	590:603	Herein, we investigate the phase behavior and thermo-rheological properties of the composite hydrogels containing cellulose nanocrystals (up to 5% by weight) and the temperature responsive Pluronic F127.
35014435	3	22	theme	hydrogels	656:664	arg1	properties					628:637	thermo-rheological properties	609:637	thermo-rheological properties	609:637	Herein, we investigate the phase behavior and thermo-rheological properties of the composite hydrogels containing cellulose nanocrystals (up to 5% by weight) and the temperature responsive Pluronic F127.
35014435	11	23	theme	adaptive	1990:1997	arg1	biolubricants					1999:2011	smartly adaptive biolubricants	1982:2011	smartly adaptive biolubricants	1982:2011	Thus, they can be used as smartly adaptive biolubricants and bioviscostatic materials.
35014435	11	23	theme	adaptive	1990:1997	arg1	they					1962:1965	they	1962:1965	they	1962:1965	Thus, they can be used as smartly adaptive biolubricants and bioviscostatic materials.
35014435	5	24	dep	loadings	988:995	arg1	%					1001:1001	1-3%	998:1001	1-3% by weight	998:1011	Linear and nonlinear rheological analysis suggest that at low and moderate nanocrystal loadings (1-3% by weight), the composite gel remarkably becomes softer and deformable compared to the neat Pluronic F127 gels.
35014435	10	25	theme	neat	1946:1949	arg1	CNC					1951:1953	the aqueous neat CNC	1934:1953	the aqueous neat CNC	1934:1953	On the other hand, at higher CNC concentrations, abrupt viscosity transition is not observed, rather the composite gels smoothly thicken with temperature in contrast to thermal thinning of the aqueous neat CNC.
35014435	0	26	theme	F127	91:94	arg1	Hydrogels					42:50	Thermoresponsive and Injectable Composite Hydrogels	0:50	Thermoresponsive and Injectable Composite Hydrogels of Cellulose Nanocrystals and Pluronic F127.	0:95	Thermoresponsive and Injectable Composite Hydrogels of Cellulose Nanocrystals and Pluronic F127.
35014435	10	27	dep	thinning	1922:1929	arg1	contrast					1902:1909	contrast	1902:1909	contrast	1902:1909	On the other hand, at higher CNC concentrations, abrupt viscosity transition is not observed, rather the composite gels smoothly thicken with temperature in contrast to thermal thinning of the aqueous neat CNC.
35014435	4	28	from	role	805:808	arg1	micellization					838:850	micellization	838:850	micellization	838:850	Our results revealed an unprecedented role of CNC network formation on micellization and gelation behavior of the triblock copolymer.
35014435	4	28	from	role	805:808	arg1	behavior					865:872	gelation behavior	856:872	gelation behavior	856:872	Our results revealed an unprecedented role of CNC network formation on micellization and gelation behavior of the triblock copolymer.
35014435	2	29	theme	rheological	539:549	arg1	properties					551:560	superior rheological properties	530:560	superior rheological properties	530:560	Meanwhile, cellulose nanocrystals (CNCs) have quickly become the focus of many drug delivery and tissue engineering applications due to their biocompatibility, abundance, ability to conjugate with drug molecules, and superior rheological properties.
35014435	4	30	theme	CNC	813:815	arg1	formation					825:833	CNC network formation	813:833	CNC network formation	813:833	Our results revealed an unprecedented role of CNC network formation on micellization and gelation behavior of the triblock copolymer.
35014435	2	31	theme	engineering	417:427	arg1	focus					378:382	the focus	374:382	the focus of many drug delivery and tissue engineering applications due to their biocompatibility, abundance, ability to conjugate with drug molecules, and superior rheological properties	374:560	Meanwhile, cellulose nanocrystals (CNCs) have quickly become the focus of many drug delivery and tissue engineering applications due to their biocompatibility, abundance, ability to conjugate with drug molecules, and superior rheological properties.
35014435	0	32	theme	Thermoresponsive	0:15	arg1	Hydrogels					42:50	Thermoresponsive and Injectable Composite Hydrogels	0:50	Thermoresponsive and Injectable Composite Hydrogels of Cellulose Nanocrystals and Pluronic F127.	0:95	Thermoresponsive and Injectable Composite Hydrogels of Cellulose Nanocrystals and Pluronic F127.
35014435	1	33	theme	amphiphilic	114:124	arg1	solutions					159:167	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions	97:167	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions	97:167	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions have been widely investigated in smart biomaterial applications due to the proximity of its critical gel temperature to human body temperature.
35014435	9	34	theme	drug	1722:1725	arg1	systems					1736:1742	smart drug delivery systems	1716:1742	smart drug delivery systems	1716:1742	Our temperature sweep experiments show that the CNC addition up to 3% does not change the rapid thermal gelation of the F127 solutions; therefore, these composites are suitable for smart drug delivery systems.
35014435	8	35	theme	F127	1371:1374	arg1	modulus					1386:1392	the original (neat F127) hard-gel modulus	1352:1392	the original (neat F127) hard-gel modulus	1352:1392	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	8	35	theme	F127	1371:1374	arg1	result					1344:1349	a result	1342:1349	a result	1342:1349	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	4	36	theme	gelation	856:863	arg1	behavior					865:872	gelation behavior	856:872	gelation behavior	856:872	Our results revealed an unprecedented role of CNC network formation on micellization and gelation behavior of the triblock copolymer.
35014435	0	37	theme	Injectable	21:30	arg1	Hydrogels					42:50	Thermoresponsive and Injectable Composite Hydrogels	0:50	Thermoresponsive and Injectable Composite Hydrogels of Cellulose Nanocrystals and Pluronic F127.	0:95	Thermoresponsive and Injectable Composite Hydrogels of Cellulose Nanocrystals and Pluronic F127.
35014435	1	38	theme	F127	135:138	arg1	solutions					159:167	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions	97:167	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions	97:167	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions have been widely investigated in smart biomaterial applications due to the proximity of its critical gel temperature to human body temperature.
35014435	8	39	theme	hard-gel	1377:1384	arg1	modulus					1386:1392	the original (neat F127) hard-gel modulus	1352:1392	the original (neat F127) hard-gel modulus	1352:1392	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	8	39	theme	hard-gel	1377:1384	arg1	result					1344:1349	a result	1342:1349	a result	1342:1349	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	2	40	theme	due	442:444	arg1	delivery					397:404	many drug delivery	387:404	many drug delivery	387:404	Meanwhile, cellulose nanocrystals (CNCs) have quickly become the focus of many drug delivery and tissue engineering applications due to their biocompatibility, abundance, ability to conjugate with drug molecules, and superior rheological properties.
35014435	1	41	theme	smart	202:206	arg1	applications					220:231	smart biomaterial applications	202:231	smart biomaterial applications due to the proximity of its critical gel temperature to human body temperature	202:310	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions have been widely investigated in smart biomaterial applications due to the proximity of its critical gel temperature to human body temperature.
35014435	10	42	theme	other	1752:1756	arg1	hand					1758:1761	the other hand	1748:1761	the other hand	1748:1761	On the other hand, at higher CNC concentrations, abrupt viscosity transition is not observed, rather the composite gels smoothly thicken with temperature in contrast to thermal thinning of the aqueous neat CNC.
35014435	11	43	theme	bioviscostatic	2017:2030	arg1	materials					2032:2040	bioviscostatic materials	2017:2040	bioviscostatic materials	2017:2040	Thus, they can be used as smartly adaptive biolubricants and bioviscostatic materials.
35014435	11	43	theme	bioviscostatic	2017:2030	arg1	they					1962:1965	they	1962:1965	they	1962:1965	Thus, they can be used as smartly adaptive biolubricants and bioviscostatic materials.
35014435	8	44	from	deformable	1474:1483	arg1	presence					1506:1513	the presence	1502:1513	the presence of the CNC network	1502:1532	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	9	45	theme	sweep	1551:1555	arg1	experiments					1557:1567	Our temperature sweep experiments	1535:1567	Our temperature sweep experiments	1535:1567	Our temperature sweep experiments show that the CNC addition up to 3% does not change the rapid thermal gelation of the F127 solutions; therefore, these composites are suitable for smart drug delivery systems.
35014435	8	46	theme	CNC	1522:1524	arg1	network					1526:1532	the CNC network	1518:1532	the CNC network	1518:1532	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	4	47	theme	formation	825:833	arg1	role					805:808	an unprecedented role	788:808	an unprecedented role of CNC network formation on micellization and gelation behavior of the triblock copolymer	788:898	Our results revealed an unprecedented role of CNC network formation on micellization and gelation behavior of the triblock copolymer.
35014435	3	48	dep	nanocrystals	687:698	arg1	%					708:708	up to 5%	701:708	up to 5%	701:708	Herein, we investigate the phase behavior and thermo-rheological properties of the composite hydrogels containing cellulose nanocrystals (up to 5% by weight) and the temperature responsive Pluronic F127.
35014435	5	49	theme	composite	1019:1027	arg1	gel					1029:1031	the composite gel	1015:1031	the composite gel	1015:1031	Linear and nonlinear rheological analysis suggest that at low and moderate nanocrystal loadings (1-3% by weight), the composite gel remarkably becomes softer and deformable compared to the neat Pluronic F127 gels.
35014435	10	50	theme	higher	1767:1772	arg1	concentrations					1778:1791	higher CNC concentrations	1767:1791	higher CNC concentrations	1767:1791	On the other hand, at higher CNC concentrations, abrupt viscosity transition is not observed, rather the composite gels smoothly thicken with temperature in contrast to thermal thinning of the aqueous neat CNC.
35014435	2	51	theme	drug	392:395	arg1	delivery					397:404	many drug delivery	387:404	many drug delivery	387:404	Meanwhile, cellulose nanocrystals (CNCs) have quickly become the focus of many drug delivery and tissue engineering applications due to their biocompatibility, abundance, ability to conjugate with drug molecules, and superior rheological properties.
35014435	1	52	theme	gel	270:272	arg1	temperature					274:284	its critical gel temperature	257:284	its critical gel temperature	257:284	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions have been widely investigated in smart biomaterial applications due to the proximity of its critical gel temperature to human body temperature.
35014435	9	53	dep	3	1602:1602	arg1	to					1599:1600	to	1599:1600	to	1599:1600	Our temperature sweep experiments show that the CNC addition up to 3% does not change the rapid thermal gelation of the F127 solutions; therefore, these composites are suitable for smart drug delivery systems.
35014435	7	54	theme	own	1275:1277	arg1	network					1279:1285	their own network	1269:1285	their own network	1269:1285	At high concentrations, however, the nanocrystals form their own network and the micelles are trapped within the CNC meshes.
35014435	3	55	contain	containing	666:675	arg1	hydrogels					656:664	the composite hydrogels	642:664	the composite hydrogels containing cellulose nanocrystals (up to 5% by weight)	642:719	Herein, we investigate the phase behavior and thermo-rheological properties of the composite hydrogels containing cellulose nanocrystals (up to 5% by weight) and the temperature responsive Pluronic F127.
35014435	3	55	contain	containing	666:675	arg2	nanocrystals					687:698	cellulose nanocrystals	677:698	cellulose nanocrystals (up to 5% by weight)	677:719	Herein, we investigate the phase behavior and thermo-rheological properties of the composite hydrogels containing cellulose nanocrystals (up to 5% by weight) and the temperature responsive Pluronic F127.
35014435	8	56	from	presence	1506:1513	arg1	gel					1457:1459	the composite gel	1443:1459	the composite gel	1443:1459	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	8	56	from	presence	1506:1513	arg1	deformable					1474:1483	deformable	1474:1483	deformable	1474:1483	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	8	56	from	presence	1506:1513	arg1	tougher					1490:1496	tougher	1490:1496	tougher	1490:1496	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	3	57	dep	5	707:707	arg1	to					704:705	to	704:705	to	704:705	Herein, we investigate the phase behavior and thermo-rheological properties of the composite hydrogels containing cellulose nanocrystals (up to 5% by weight) and the temperature responsive Pluronic F127.
35014435	10	58	theme	abrupt	1794:1799	arg1	transition					1811:1820	abrupt viscosity transition	1794:1820	abrupt viscosity transition	1794:1820	On the other hand, at higher CNC concentrations, abrupt viscosity transition is not observed, rather the composite gels smoothly thicken with temperature in contrast to thermal thinning of the aqueous neat CNC.
35014435	2	59	theme	cellulose	324:332	arg1	CNCs					348:351	CNCs	348:351	CNCs	348:351	Meanwhile, cellulose nanocrystals (CNCs) have quickly become the focus of many drug delivery and tissue engineering applications due to their biocompatibility, abundance, ability to conjugate with drug molecules, and superior rheological properties.
35014435	2	59	theme	cellulose	324:332	arg1	nanocrystals					334:345	cellulose nanocrystals	324:345	cellulose nanocrystals (CNCs)	324:352	Meanwhile, cellulose nanocrystals (CNCs) have quickly become the focus of many drug delivery and tissue engineering applications due to their biocompatibility, abundance, ability to conjugate with drug molecules, and superior rheological properties.
35014435	1	60	theme	body	295:298	arg1	temperature					300:310	human body temperature	289:310	human body temperature	289:310	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions have been widely investigated in smart biomaterial applications due to the proximity of its critical gel temperature to human body temperature.
35014435	11	61	theme	smartly	1982:1988	arg1	biolubricants					1999:2011	smartly adaptive biolubricants	1982:2011	smartly adaptive biolubricants	1982:2011	Thus, they can be used as smartly adaptive biolubricants and bioviscostatic materials.
35014435	11	61	theme	smartly	1982:1988	arg1	they					1962:1965	they	1962:1965	they	1962:1965	Thus, they can be used as smartly adaptive biolubricants and bioviscostatic materials.
35014435	6	62	theme	rodlike	1200:1206	arg1	CNCs					1208:1211	the rodlike CNCs	1196:1211	the rodlike CNCs	1196:1211	The softening effect results from the disruption of the close packed micelles by the rodlike CNCs.
35014435	5	63	theme	Linear	901:906	arg1	analysis					934:941	Linear and nonlinear rheological analysis	901:941	Linear and nonlinear rheological analysis	901:941	Linear and nonlinear rheological analysis suggest that at low and moderate nanocrystal loadings (1-3% by weight), the composite gel remarkably becomes softer and deformable compared to the neat Pluronic F127 gels.
35014435	0	64	theme	Cellulose	55:63	arg1	Nanocrystals					65:76	Cellulose Nanocrystals	55:76	Cellulose Nanocrystals	55:76	Thermoresponsive and Injectable Composite Hydrogels of Cellulose Nanocrystals and Pluronic F127.
35014435	3	65	theme	Pluronic	752:759	arg1	F127					761:764	the temperature responsive Pluronic F127	725:764	the temperature responsive Pluronic F127	725:764	Herein, we investigate the phase behavior and thermo-rheological properties of the composite hydrogels containing cellulose nanocrystals (up to 5% by weight) and the temperature responsive Pluronic F127.
35014435	10	66	located	observed	1829:1836	arg1	concentrations					1778:1791	higher CNC concentrations	1767:1791	higher CNC concentrations	1767:1791	On the other hand, at higher CNC concentrations, abrupt viscosity transition is not observed, rather the composite gels smoothly thicken with temperature in contrast to thermal thinning of the aqueous neat CNC.
35014435	10	66	located	observed	1829:1836	arg1	hand					1758:1761	the other hand	1748:1761	the other hand	1748:1761	On the other hand, at higher CNC concentrations, abrupt viscosity transition is not observed, rather the composite gels smoothly thicken with temperature in contrast to thermal thinning of the aqueous neat CNC.
35014435	10	66	located	observed	1829:1836	arg2	transition					1811:1820	abrupt viscosity transition	1794:1820	abrupt viscosity transition	1794:1820	On the other hand, at higher CNC concentrations, abrupt viscosity transition is not observed, rather the composite gels smoothly thicken with temperature in contrast to thermal thinning of the aqueous neat CNC.
35014435	5	67	theme	nonlinear	912:920	arg1	analysis					934:941	Linear and nonlinear rheological analysis	901:941	Linear and nonlinear rheological analysis	901:941	Linear and nonlinear rheological analysis suggest that at low and moderate nanocrystal loadings (1-3% by weight), the composite gel remarkably becomes softer and deformable compared to the neat Pluronic F127 gels.
35014435	4	68	theme	copolymer	890:898	arg1	micellization					838:850	micellization	838:850	micellization	838:850	Our results revealed an unprecedented role of CNC network formation on micellization and gelation behavior of the triblock copolymer.
35014435	4	68	theme	copolymer	890:898	arg1	behavior					865:872	gelation behavior	856:872	gelation behavior	856:872	Our results revealed an unprecedented role of CNC network formation on micellization and gelation behavior of the triblock copolymer.
35014435	6	69	theme	packed	1177:1182	arg1	micelles					1184:1191	the close packed micelles	1167:1191	the close packed micelles	1167:1191	The softening effect results from the disruption of the close packed micelles by the rodlike CNCs.
35014435	5	70	theme	F127	1104:1107	arg1	gels					1109:1112	the neat Pluronic F127 gels	1086:1112	the neat Pluronic F127 gels	1086:1112	Linear and nonlinear rheological analysis suggest that at low and moderate nanocrystal loadings (1-3% by weight), the composite gel remarkably becomes softer and deformable compared to the neat Pluronic F127 gels.
35014435	5	71	theme	rheological	922:932	arg1	analysis					934:941	Linear and nonlinear rheological analysis	901:941	Linear and nonlinear rheological analysis	901:941	Linear and nonlinear rheological analysis suggest that at low and moderate nanocrystal loadings (1-3% by weight), the composite gel remarkably becomes softer and deformable compared to the neat Pluronic F127 gels.
35014435	9	72	theme	thermal	1631:1637	arg1	gelation					1639:1646	the rapid thermal gelation	1621:1646	the rapid thermal gelation of the F127 solutions	1621:1668	Our temperature sweep experiments show that the CNC addition up to 3% does not change the rapid thermal gelation of the F127 solutions; therefore, these composites are suitable for smart drug delivery systems.
35014435	3	73	theme	thermo-rheological	609:626	arg1	properties					628:637	thermo-rheological properties	609:637	thermo-rheological properties	609:637	Herein, we investigate the phase behavior and thermo-rheological properties of the composite hydrogels containing cellulose nanocrystals (up to 5% by weight) and the temperature responsive Pluronic F127.
35014435	5	74	theme	low	959:961	arg1	loadings					988:995	low and moderate nanocrystal loadings	959:995	low and moderate nanocrystal loadings (1-3% by weight)	959:1012	Linear and nonlinear rheological analysis suggest that at low and moderate nanocrystal loadings (1-3% by weight), the composite gel remarkably becomes softer and deformable compared to the neat Pluronic F127 gels.
35014435	5	75	theme	moderate	967:974	arg1	loadings					988:995	low and moderate nanocrystal loadings	959:995	low and moderate nanocrystal loadings (1-3% by weight)	959:1012	Linear and nonlinear rheological analysis suggest that at low and moderate nanocrystal loadings (1-3% by weight), the composite gel remarkably becomes softer and deformable compared to the neat Pluronic F127 gels.
35014435	9	76	theme	F127	1655:1658	arg1	solutions					1660:1668	the F127 solutions	1651:1668	the F127 solutions	1651:1668	Our temperature sweep experiments show that the CNC addition up to 3% does not change the rapid thermal gelation of the F127 solutions; therefore, these composites are suitable for smart drug delivery systems.
35014435	8	77	theme	composite	1447:1455	arg1	gel					1457:1459	the composite gel	1443:1459	the composite gel	1443:1459	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	8	77	theme	composite	1447:1455	arg1	deformable					1474:1483	deformable	1474:1483	deformable	1474:1483	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	8	77	theme	composite	1447:1455	arg1	tougher					1490:1496	tougher	1490:1496	tougher	1490:1496	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	10	78	theme	thermal	1914:1920	arg1	thinning					1922:1929	thermal thinning	1914:1929	thermal thinning of the aqueous neat CNC	1914:1953	On the other hand, at higher CNC concentrations, abrupt viscosity transition is not observed, rather the composite gels smoothly thicken with temperature in contrast to thermal thinning of the aqueous neat CNC.
35014435	0	79	theme	Pluronic	82:89	arg1	F127					91:94	Pluronic F127	82:94	Pluronic F127	82:94	Thermoresponsive and Injectable Composite Hydrogels of Cellulose Nanocrystals and Pluronic F127.
35014435	5	80	theme	neat	1090:1093	arg1	gels					1109:1112	the neat Pluronic F127 gels	1086:1112	the neat Pluronic F127 gels	1086:1112	Linear and nonlinear rheological analysis suggest that at low and moderate nanocrystal loadings (1-3% by weight), the composite gel remarkably becomes softer and deformable compared to the neat Pluronic F127 gels.
35014435	3	81	theme	composite	646:654	arg1	hydrogels					656:664	the composite hydrogels	642:664	the composite hydrogels containing cellulose nanocrystals (up to 5% by weight)	642:719	Herein, we investigate the phase behavior and thermo-rheological properties of the composite hydrogels containing cellulose nanocrystals (up to 5% by weight) and the temperature responsive Pluronic F127.
35014435	2	82	with	conjugate	495:503	arg1	molecules					515:523	drug molecules	510:523	drug molecules	510:523	Meanwhile, cellulose nanocrystals (CNCs) have quickly become the focus of many drug delivery and tissue engineering applications due to their biocompatibility, abundance, ability to conjugate with drug molecules, and superior rheological properties.
35014435	3	83	dep	behavior	596:603	arg1	the					586:588	the	586:588	the	586:588	Herein, we investigate the phase behavior and thermo-rheological properties of the composite hydrogels containing cellulose nanocrystals (up to 5% by weight) and the temperature responsive Pluronic F127.
35014435	2	84	theme	drug	510:513	arg1	molecules					515:523	drug molecules	510:523	drug molecules	510:523	Meanwhile, cellulose nanocrystals (CNCs) have quickly become the focus of many drug delivery and tissue engineering applications due to their biocompatibility, abundance, ability to conjugate with drug molecules, and superior rheological properties.
35014435	10	85	theme	aqueous	1938:1944	arg1	CNC					1951:1953	the aqueous neat CNC	1934:1953	the aqueous neat CNC	1934:1953	On the other hand, at higher CNC concentrations, abrupt viscosity transition is not observed, rather the composite gels smoothly thicken with temperature in contrast to thermal thinning of the aqueous neat CNC.
35014435	7	86	theme	high	1217:1220	arg1	concentrations					1222:1235	high concentrations	1217:1235	high concentrations	1217:1235	At high concentrations, however, the nanocrystals form their own network and the micelles are trapped within the CNC meshes.
35014435	2	87	dep	delivery	397:404	arg1	applications					429:440	applications	429:440	applications	429:440	Meanwhile, cellulose nanocrystals (CNCs) have quickly become the focus of many drug delivery and tissue engineering applications due to their biocompatibility, abundance, ability to conjugate with drug molecules, and superior rheological properties.
35014435	4	88	theme	unprecedented	791:803	arg1	role					805:808	an unprecedented role	788:808	an unprecedented role of CNC network formation on micellization and gelation behavior of the triblock copolymer	788:898	Our results revealed an unprecedented role of CNC network formation on micellization and gelation behavior of the triblock copolymer.
35014435	2	89	theme	superior	530:537	arg1	properties					551:560	superior rheological properties	530:560	superior rheological properties	530:560	Meanwhile, cellulose nanocrystals (CNCs) have quickly become the focus of many drug delivery and tissue engineering applications due to their biocompatibility, abundance, ability to conjugate with drug molecules, and superior rheological properties.
35014435	2	90	theme	tissue	410:415	arg1	engineering					417:427	tissue engineering	410:427	tissue engineering	410:427	Meanwhile, cellulose nanocrystals (CNCs) have quickly become the focus of many drug delivery and tissue engineering applications due to their biocompatibility, abundance, ability to conjugate with drug molecules, and superior rheological properties.
35014435	1	91	theme	Thermoresponsive	97:112	arg1	solutions					159:167	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions	97:167	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions	97:167	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions have been widely investigated in smart biomaterial applications due to the proximity of its critical gel temperature to human body temperature.
35014435	7	92	theme	CNC	1327:1329	arg1	meshes					1331:1336	the CNC meshes	1323:1336	the CNC meshes	1323:1336	At high concentrations, however, the nanocrystals form their own network and the micelles are trapped within the CNC meshes.
35014435	9	93	theme	smart	1716:1720	arg1	systems					1736:1742	smart drug delivery systems	1716:1742	smart drug delivery systems	1716:1742	Our temperature sweep experiments show that the CNC addition up to 3% does not change the rapid thermal gelation of the F127 solutions; therefore, these composites are suitable for smart drug delivery systems.
35014435	10	94	dep	observed	1829:1836	arg1	thicken					1874:1880	thicken	1874:1880	thicken with temperature in contrast to thermal thinning of the aqueous neat CNC	1874:1953	On the other hand, at higher CNC concentrations, abrupt viscosity transition is not observed, rather the composite gels smoothly thicken with temperature in contrast to thermal thinning of the aqueous neat CNC.
35014435	8	95	theme	original	1356:1363	arg1	modulus					1386:1392	the original (neat F127) hard-gel modulus	1352:1392	the original (neat F127) hard-gel modulus	1352:1392	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	8	95	theme	original	1356:1363	arg1	result					1344:1349	a result	1342:1349	a result	1342:1349	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	2	96	theme	delivery	397:404	arg1	focus					378:382	the focus	374:382	the focus of many drug delivery and tissue engineering applications due to their biocompatibility, abundance, ability to conjugate with drug molecules, and superior rheological properties	374:560	Meanwhile, cellulose nanocrystals (CNCs) have quickly become the focus of many drug delivery and tissue engineering applications due to their biocompatibility, abundance, ability to conjugate with drug molecules, and superior rheological properties.
35014435	0	97	theme	Composite	32:40	arg1	Hydrogels					42:50	Thermoresponsive and Injectable Composite Hydrogels	0:50	Thermoresponsive and Injectable Composite Hydrogels of Cellulose Nanocrystals and Pluronic F127.	0:95	Thermoresponsive and Injectable Composite Hydrogels of Cellulose Nanocrystals and Pluronic F127.
35014435	1	98	theme	Pluronic	126:133	arg1	solutions					159:167	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions	97:167	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions	97:167	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions have been widely investigated in smart biomaterial applications due to the proximity of its critical gel temperature to human body temperature.
35014435	9	99	theme	delivery	1727:1734	arg1	systems					1736:1742	smart drug delivery systems	1716:1742	smart drug delivery systems	1716:1742	Our temperature sweep experiments show that the CNC addition up to 3% does not change the rapid thermal gelation of the F127 solutions; therefore, these composites are suitable for smart drug delivery systems.
35014435	8	100	theme	neat	1366:1369	arg1	modulus					1386:1392	the original (neat F127) hard-gel modulus	1352:1392	the original (neat F127) hard-gel modulus	1352:1392	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	8	100	theme	neat	1366:1369	arg1	result					1344:1349	a result	1342:1349	a result	1342:1349	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	0	101	theme	Nanocrystals	65:76	arg1	Hydrogels					42:50	Thermoresponsive and Injectable Composite Hydrogels	0:50	Thermoresponsive and Injectable Composite Hydrogels of Cellulose Nanocrystals and Pluronic F127.	0:95	Thermoresponsive and Injectable Composite Hydrogels of Cellulose Nanocrystals and Pluronic F127.
35014435	9	102	theme	temperature	1539:1549	arg1	experiments					1557:1567	Our temperature sweep experiments	1535:1567	Our temperature sweep experiments	1535:1567	Our temperature sweep experiments show that the CNC addition up to 3% does not change the rapid thermal gelation of the F127 solutions; therefore, these composites are suitable for smart drug delivery systems.
35014435	8	103	theme	network	1526:1532	arg1	presence					1506:1513	the presence	1502:1513	the presence of the CNC network	1502:1532	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	8	104	dep	5	1415:1415	arg1	to					1412:1413	to	1412:1413	to	1412:1413	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	10	105	theme	CNC	1774:1776	arg1	concentrations					1778:1791	higher CNC concentrations	1767:1791	higher CNC concentrations	1767:1791	On the other hand, at higher CNC concentrations, abrupt viscosity transition is not observed, rather the composite gels smoothly thicken with temperature in contrast to thermal thinning of the aqueous neat CNC.
35014435	4	106	theme	network	817:823	arg1	formation					825:833	CNC network formation	813:833	CNC network formation	813:833	Our results revealed an unprecedented role of CNC network formation on micellization and gelation behavior of the triblock copolymer.
35014435	1	107	theme	critical	261:268	arg1	temperature					274:284	its critical gel temperature	257:284	its critical gel temperature	257:284	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions have been widely investigated in smart biomaterial applications due to the proximity of its critical gel temperature to human body temperature.
35014435	10	108	theme	CNC	1951:1953	arg1	thinning					1922:1929	thermal thinning	1914:1929	thermal thinning of the aqueous neat CNC	1914:1953	On the other hand, at higher CNC concentrations, abrupt viscosity transition is not observed, rather the composite gels smoothly thicken with temperature in contrast to thermal thinning of the aqueous neat CNC.
35014435	1	109	theme	temperature	274:284	arg1	proximity					244:252	the proximity	240:252	the proximity of its critical gel temperature to human body temperature	240:310	Thermoresponsive amphiphilic Pluronic F127 triblock copolymer solutions have been widely investigated in smart biomaterial applications due to the proximity of its critical gel temperature to human body temperature.
35014435	2	110	theme	many	387:390	arg1	delivery					397:404	many drug delivery	387:404	many drug delivery	387:404	Meanwhile, cellulose nanocrystals (CNCs) have quickly become the focus of many drug delivery and tissue engineering applications due to their biocompatibility, abundance, ability to conjugate with drug molecules, and superior rheological properties.
35014435	8	111	dep	deformable	1474:1483	arg1	gel					1457:1459	the composite gel	1443:1459	the composite gel	1443:1459	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	8	111	dep	deformable	1474:1483	arg1	deformable					1474:1483	deformable	1474:1483	deformable	1474:1483	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
35014435	8	111	dep	deformable	1474:1483	arg1	tougher					1490:1496	tougher	1490:1496	tougher	1490:1496	As a result, the original (neat F127) hard-gel modulus is recovered at 4 to 5% nanocrystal loading, yet the composite gel is much more deformable (and tougher) in the presence of the CNC network.
33357838	0	0	with	composite	51:59	arg1	shielding					91:99	superior electromagnetic shielding	66:99	superior electromagnetic shielding	66:99	MXene/wood-derived hierarchical cellulose scaffold composite with superior electromagnetic shielding.
33357838	1	1	theme	radiation	283:291	arg1	pollution					293:301	increasingly severe radiation pollution	263:301	increasingly severe radiation pollution	263:301	Electromagnetic-interference (EMI) shielding materials that are green, lightweight, and with high mechanical properties need to be urgently developed to address increasingly severe radiation pollution.
33357838	5	2	theme	shielding	1033:1041	arg1	materials					1043:1051	robust and sustainable EMI shielding materials	1006:1051	robust and sustainable EMI shielding materials	1006:1051	This "top-down" method provides an alternative for the efficient production of robust and sustainable EMI shielding materials that can be used in the fields of structural materials for next-generation communications and electronic devices.
33357838	2	3	theme	due	394:396	arg1	applications					380:391	practical applications	370:391	practical applications	370:391	However, limited EMI shielding materials are successfully used in practical applications, due to the intensive energy consumption or the absence of sufficient strength.
33357838	3	4	with	composites	565:574	arg1	robustness					597:606	high mechanical robustness	581:606	high mechanical robustness	581:606	Herein, an environmentally friendly and effective method was proved to fabricate wood-based composites with high mechanical robustness and EMI shielding performance by a MXene/cellulose scaffold assembly strategy.
33357838	3	4	with	composites	565:574	arg1	performance					626:636	EMI shielding performance	612:636	EMI shielding performance	612:636	Herein, an environmentally friendly and effective method was proved to fabricate wood-based composites with high mechanical robustness and EMI shielding performance by a MXene/cellulose scaffold assembly strategy.
33357838	2	5	theme	shielding	325:333	arg1	materials					335:343	limited EMI shielding materials	313:343	limited EMI shielding materials	313:343	However, limited EMI shielding materials are successfully used in practical applications, due to the intensive energy consumption or the absence of sufficient strength.
33357838	3	6	theme	shielding	616:624	arg1	performance					626:636	EMI shielding performance	612:636	EMI shielding performance	612:636	Herein, an environmentally friendly and effective method was proved to fabricate wood-based composites with high mechanical robustness and EMI shielding performance by a MXene/cellulose scaffold assembly strategy.
33357838	5	7	theme	robust	1006:1011	arg1	materials					1043:1051	robust and sustainable EMI shielding materials	1006:1051	robust and sustainable EMI shielding materials	1006:1051	This "top-down" method provides an alternative for the efficient production of robust and sustainable EMI shielding materials that can be used in the fields of structural materials for next-generation communications and electronic devices.
33357838	2	8	theme	EMI	321:323	arg1	materials					335:343	limited EMI shielding materials	313:343	limited EMI shielding materials	313:343	However, limited EMI shielding materials are successfully used in practical applications, due to the intensive energy consumption or the absence of sufficient strength.
33357838	5	9	used	used	1065:1068	arg2	alternative					962:972	an alternative	959:972	an alternative for the efficient production of robust and sustainable EMI shielding materials that can be used in the fields of structural materials for next-generation communications and electronic devices	959:1164	This "top-down" method provides an alternative for the efficient production of robust and sustainable EMI shielding materials that can be used in the fields of structural materials for next-generation communications and electronic devices.
33357838	5	10	theme	efficient	982:990	arg1	production					992:1001	the efficient production	978:1001	the efficient production of robust and sustainable EMI shielding materials	978:1051	This "top-down" method provides an alternative for the efficient production of robust and sustainable EMI shielding materials that can be used in the fields of structural materials for next-generation communications and electronic devices.
33357838	5	11	theme	sustainable	1017:1027	arg1	materials					1043:1051	robust and sustainable EMI shielding materials	1006:1051	robust and sustainable EMI shielding materials	1006:1051	This "top-down" method provides an alternative for the efficient production of robust and sustainable EMI shielding materials that can be used in the fields of structural materials for next-generation communications and electronic devices.
33357838	0	12	link	MXene/wood-derived	0:17	arg1	scaffold					42:49	MXene/wood-derived hierarchical cellulose scaffold	0:49	MXene/wood-derived hierarchical cellulose scaffold	0:49	MXene/wood-derived hierarchical cellulose scaffold composite with superior electromagnetic shielding.
33357838	1	13	theme	Electromagnetic-interference	102:129	arg1	materials					147:155	Electromagnetic-interference (EMI) shielding materials	102:155	Electromagnetic-interference (EMI) shielding materials that are green, lightweight, and with high mechanical properties	102:220	Electromagnetic-interference (EMI) shielding materials that are green, lightweight, and with high mechanical properties need to be urgently developed to address increasingly severe radiation pollution.
33357838	1	14	theme	high	195:198	arg1	properties					211:220	high mechanical properties	195:220	high mechanical properties	195:220	Electromagnetic-interference (EMI) shielding materials that are green, lightweight, and with high mechanical properties need to be urgently developed to address increasingly severe radiation pollution.
33357838	4	15	theme	dB	923:924	arg1	effectiveness					901:913	EMI shielding effectiveness	887:913	EMI shielding effectiveness of 39.3 dB	887:924	The lignocellulose composites with a millimeter-thick mimic the "mortar-brick" layered structure, resulting in excellent mechanical properties that can achieve the compressive strength of 288 MPa and EMI shielding effectiveness of 39.3 dB.
33357838	4	15	theme	dB	923:924	arg1	strength					863:870	the compressive strength	847:870	the compressive strength of 288 MPa	847:881	The lignocellulose composites with a millimeter-thick mimic the "mortar-brick" layered structure, resulting in excellent mechanical properties that can achieve the compressive strength of 288 MPa and EMI shielding effectiveness of 39.3 dB.
33357838	3	16	theme	mechanical	586:595	arg1	robustness					597:606	high mechanical robustness	581:606	high mechanical robustness	581:606	Herein, an environmentally friendly and effective method was proved to fabricate wood-based composites with high mechanical robustness and EMI shielding performance by a MXene/cellulose scaffold assembly strategy.
33357838	1	17	theme	mechanical	200:209	arg1	properties					211:220	high mechanical properties	195:220	high mechanical properties	195:220	Electromagnetic-interference (EMI) shielding materials that are green, lightweight, and with high mechanical properties need to be urgently developed to address increasingly severe radiation pollution.
33357838	0	18	theme	hierarchical	19:30	arg1	scaffold					42:49	MXene/wood-derived hierarchical cellulose scaffold	0:49	MXene/wood-derived hierarchical cellulose scaffold	0:49	MXene/wood-derived hierarchical cellulose scaffold composite with superior electromagnetic shielding.
33357838	1	19	theme	EMI	132:134	arg1	materials					147:155	Electromagnetic-interference (EMI) shielding materials	102:155	Electromagnetic-interference (EMI) shielding materials that are green, lightweight, and with high mechanical properties	102:220	Electromagnetic-interference (EMI) shielding materials that are green, lightweight, and with high mechanical properties need to be urgently developed to address increasingly severe radiation pollution.
33357838	3	20	theme	high	581:584	arg1	robustness					597:606	high mechanical robustness	581:606	high mechanical robustness	581:606	Herein, an environmentally friendly and effective method was proved to fabricate wood-based composites with high mechanical robustness and EMI shielding performance by a MXene/cellulose scaffold assembly strategy.
33357838	4	21	theme	mortar-brick	752:763	arg1	structure					774:782	the "mortar-brick" layered structure	747:782	the "mortar-brick" layered structure	747:782	The lignocellulose composites with a millimeter-thick mimic the "mortar-brick" layered structure, resulting in excellent mechanical properties that can achieve the compressive strength of 288 MPa and EMI shielding effectiveness of 39.3 dB.
33357838	0	22	theme	MXene/wood-derived	0:17	arg1	scaffold					42:49	MXene/wood-derived hierarchical cellulose scaffold	0:49	MXene/wood-derived hierarchical cellulose scaffold	0:49	MXene/wood-derived hierarchical cellulose scaffold composite with superior electromagnetic shielding.
33357838	5	23	theme	electronic	1147:1156	arg1	devices					1158:1164	electronic devices	1147:1164	electronic devices	1147:1164	This "top-down" method provides an alternative for the efficient production of robust and sustainable EMI shielding materials that can be used in the fields of structural materials for next-generation communications and electronic devices.
33357838	5	24	theme	top-down	933:940	arg1	method					943:948	This "top-down" method	927:948	This "top-down" method	927:948	This "top-down" method provides an alternative for the efficient production of robust and sustainable EMI shielding materials that can be used in the fields of structural materials for next-generation communications and electronic devices.
33357838	5	25	theme	"	941:941	arg1	method					943:948	This "top-down" method	927:948	This "top-down" method	927:948	This "top-down" method provides an alternative for the efficient production of robust and sustainable EMI shielding materials that can be used in the fields of structural materials for next-generation communications and electronic devices.
33357838	2	26	theme	energy	415:420	arg1	consumption					422:432	the intensive energy consumption	401:432	the intensive energy consumption	401:432	However, limited EMI shielding materials are successfully used in practical applications, due to the intensive energy consumption or the absence of sufficient strength.
33357838	3	27	theme	MXene/cellulose	643:657	arg1	strategy					677:684	a MXene/cellulose scaffold assembly strategy	641:684	a MXene/cellulose scaffold assembly strategy	641:684	Herein, an environmentally friendly and effective method was proved to fabricate wood-based composites with high mechanical robustness and EMI shielding performance by a MXene/cellulose scaffold assembly strategy.
33357838	1	28	theme	shielding	137:145	arg1	materials					147:155	Electromagnetic-interference (EMI) shielding materials	102:155	Electromagnetic-interference (EMI) shielding materials that are green, lightweight, and with high mechanical properties	102:220	Electromagnetic-interference (EMI) shielding materials that are green, lightweight, and with high mechanical properties need to be urgently developed to address increasingly severe radiation pollution.
33357838	2	29	theme	strength	463:470	arg1	consumption					422:432	the intensive energy consumption	401:432	the intensive energy consumption	401:432	However, limited EMI shielding materials are successfully used in practical applications, due to the intensive energy consumption or the absence of sufficient strength.
33357838	2	29	theme	strength	463:470	arg1	absence					441:447	the absence	437:447	the absence of sufficient strength	437:470	However, limited EMI shielding materials are successfully used in practical applications, due to the intensive energy consumption or the absence of sufficient strength.
33357838	0	30	theme	cellulose	32:40	arg1	scaffold					42:49	MXene/wood-derived hierarchical cellulose scaffold	0:49	MXene/wood-derived hierarchical cellulose scaffold	0:49	MXene/wood-derived hierarchical cellulose scaffold composite with superior electromagnetic shielding.
33357838	2	31	theme	limited	313:319	arg1	materials					335:343	limited EMI shielding materials	313:343	limited EMI shielding materials	313:343	However, limited EMI shielding materials are successfully used in practical applications, due to the intensive energy consumption or the absence of sufficient strength.
33357838	5	32	theme	EMI	1029:1031	arg1	materials					1043:1051	robust and sustainable EMI shielding materials	1006:1051	robust and sustainable EMI shielding materials	1006:1051	This "top-down" method provides an alternative for the efficient production of robust and sustainable EMI shielding materials that can be used in the fields of structural materials for next-generation communications and electronic devices.
33357838	3	33	theme	wood-based	554:563	arg1	composites					565:574	wood-based composites	554:574	wood-based composites with high mechanical robustness and EMI shielding performance	554:636	Herein, an environmentally friendly and effective method was proved to fabricate wood-based composites with high mechanical robustness and EMI shielding performance by a MXene/cellulose scaffold assembly strategy.
33357838	2	34	theme	sufficient	452:461	arg1	strength					463:470	sufficient strength	452:470	sufficient strength	452:470	However, limited EMI shielding materials are successfully used in practical applications, due to the intensive energy consumption or the absence of sufficient strength.
33357838	5	35	theme	materials	1043:1051	arg1	production					992:1001	the efficient production	978:1001	the efficient production of robust and sustainable EMI shielding materials	978:1051	This "top-down" method provides an alternative for the efficient production of robust and sustainable EMI shielding materials that can be used in the fields of structural materials for next-generation communications and electronic devices.
33357838	3	36	theme	scaffold	659:666	arg1	strategy					677:684	a MXene/cellulose scaffold assembly strategy	641:684	a MXene/cellulose scaffold assembly strategy	641:684	Herein, an environmentally friendly and effective method was proved to fabricate wood-based composites with high mechanical robustness and EMI shielding performance by a MXene/cellulose scaffold assembly strategy.
33357838	3	37	theme	environmentally	484:498	arg1	friendly					500:507	an environmentally friendly	481:507	an environmentally friendly	481:507	Herein, an environmentally friendly and effective method was proved to fabricate wood-based composites with high mechanical robustness and EMI shielding performance by a MXene/cellulose scaffold assembly strategy.
33357838	4	38	theme	layered	766:772	arg1	structure					774:782	the "mortar-brick" layered structure	747:782	the "mortar-brick" layered structure	747:782	The lignocellulose composites with a millimeter-thick mimic the "mortar-brick" layered structure, resulting in excellent mechanical properties that can achieve the compressive strength of 288 MPa and EMI shielding effectiveness of 39.3 dB.
33357838	3	39	theme	assembly	668:675	arg1	strategy					677:684	a MXene/cellulose scaffold assembly strategy	641:684	a MXene/cellulose scaffold assembly strategy	641:684	Herein, an environmentally friendly and effective method was proved to fabricate wood-based composites with high mechanical robustness and EMI shielding performance by a MXene/cellulose scaffold assembly strategy.
33357838	0	40	theme	electromagnetic	75:89	arg1	shielding					91:99	superior electromagnetic shielding	66:99	superior electromagnetic shielding	66:99	MXene/wood-derived hierarchical cellulose scaffold composite with superior electromagnetic shielding.
33357838	5	41	theme	materials	1098:1106	arg1	fields					1077:1082	the fields	1073:1082	the fields of structural materials for next-generation communications and electronic devices	1073:1164	This "top-down" method provides an alternative for the efficient production of robust and sustainable EMI shielding materials that can be used in the fields of structural materials for next-generation communications and electronic devices.
33357838	3	42	theme	effective	513:521	arg1	method					523:528	effective method	513:528	effective method	513:528	Herein, an environmentally friendly and effective method was proved to fabricate wood-based composites with high mechanical robustness and EMI shielding performance by a MXene/cellulose scaffold assembly strategy.
33357838	0	43	theme	superior	66:73	arg1	shielding					91:99	superior electromagnetic shielding	66:99	superior electromagnetic shielding	66:99	MXene/wood-derived hierarchical cellulose scaffold composite with superior electromagnetic shielding.
33357838	5	44	theme	next-generation	1112:1126	arg1	communications					1128:1141	next-generation communications	1112:1141	next-generation communications	1112:1141	This "top-down" method provides an alternative for the efficient production of robust and sustainable EMI shielding materials that can be used in the fields of structural materials for next-generation communications and electronic devices.
33357838	0	45	dep	composite	51:59	arg1	scaffold					42:49	MXene/wood-derived hierarchical cellulose scaffold	0:49	MXene/wood-derived hierarchical cellulose scaffold	0:49	MXene/wood-derived hierarchical cellulose scaffold composite with superior electromagnetic shielding.
33357838	5	46	theme	structural	1087:1096	arg1	materials					1098:1106	structural materials	1087:1106	structural materials for next-generation communications and electronic devices	1087:1164	This "top-down" method provides an alternative for the efficient production of robust and sustainable EMI shielding materials that can be used in the fields of structural materials for next-generation communications and electronic devices.
33357838	4	47	theme	MPa	879:881	arg1	effectiveness					901:913	EMI shielding effectiveness	887:913	EMI shielding effectiveness of 39.3 dB	887:924	The lignocellulose composites with a millimeter-thick mimic the "mortar-brick" layered structure, resulting in excellent mechanical properties that can achieve the compressive strength of 288 MPa and EMI shielding effectiveness of 39.3 dB.
33357838	4	47	theme	MPa	879:881	arg1	strength					863:870	the compressive strength	847:870	the compressive strength of 288 MPa	847:881	The lignocellulose composites with a millimeter-thick mimic the "mortar-brick" layered structure, resulting in excellent mechanical properties that can achieve the compressive strength of 288 MPa and EMI shielding effectiveness of 39.3 dB.
33357838	4	48	theme	shielding	891:899	arg1	effectiveness					901:913	EMI shielding effectiveness	887:913	EMI shielding effectiveness of 39.3 dB	887:924	The lignocellulose composites with a millimeter-thick mimic the "mortar-brick" layered structure, resulting in excellent mechanical properties that can achieve the compressive strength of 288 MPa and EMI shielding effectiveness of 39.3 dB.
33357838	2	49	theme	practical	370:378	arg1	applications					380:391	practical applications	370:391	practical applications	370:391	However, limited EMI shielding materials are successfully used in practical applications, due to the intensive energy consumption or the absence of sufficient strength.
33357838	3	50	theme	EMI	612:614	arg1	performance					626:636	EMI shielding performance	612:636	EMI shielding performance	612:636	Herein, an environmentally friendly and effective method was proved to fabricate wood-based composites with high mechanical robustness and EMI shielding performance by a MXene/cellulose scaffold assembly strategy.
33357838	4	51	theme	compressive	851:861	arg1	strength					863:870	the compressive strength	847:870	the compressive strength of 288 MPa	847:881	The lignocellulose composites with a millimeter-thick mimic the "mortar-brick" layered structure, resulting in excellent mechanical properties that can achieve the compressive strength of 288 MPa and EMI shielding effectiveness of 39.3 dB.
33357838	4	52	theme	excellent	798:806	arg1	properties					819:828	excellent mechanical properties	798:828	excellent mechanical properties that can achieve the compressive strength of 288 MPa and EMI shielding effectiveness of 39.3 dB	798:924	The lignocellulose composites with a millimeter-thick mimic the "mortar-brick" layered structure, resulting in excellent mechanical properties that can achieve the compressive strength of 288 MPa and EMI shielding effectiveness of 39.3 dB.
33357838	4	53	theme	mechanical	808:817	arg1	properties					819:828	excellent mechanical properties	798:828	excellent mechanical properties that can achieve the compressive strength of 288 MPa and EMI shielding effectiveness of 39.3 dB	798:924	The lignocellulose composites with a millimeter-thick mimic the "mortar-brick" layered structure, resulting in excellent mechanical properties that can achieve the compressive strength of 288 MPa and EMI shielding effectiveness of 39.3 dB.
33357838	4	54	with	composites	706:715	arg1	millimeter-thick					724:739	a millimeter-thick	722:739	a millimeter-thick	722:739	The lignocellulose composites with a millimeter-thick mimic the "mortar-brick" layered structure, resulting in excellent mechanical properties that can achieve the compressive strength of 288 MPa and EMI shielding effectiveness of 39.3 dB.
33357838	4	55	theme	EMI	887:889	arg1	effectiveness					901:913	EMI shielding effectiveness	887:913	EMI shielding effectiveness of 39.3 dB	887:924	The lignocellulose composites with a millimeter-thick mimic the "mortar-brick" layered structure, resulting in excellent mechanical properties that can achieve the compressive strength of 288 MPa and EMI shielding effectiveness of 39.3 dB.
33357838	2	56	theme	intensive	405:413	arg1	consumption					422:432	the intensive energy consumption	401:432	the intensive energy consumption	401:432	However, limited EMI shielding materials are successfully used in practical applications, due to the intensive energy consumption or the absence of sufficient strength.
33357838	2	57	used	used	362:365	arg2	materials					335:343	limited EMI shielding materials	313:343	limited EMI shielding materials	313:343	However, limited EMI shielding materials are successfully used in practical applications, due to the intensive energy consumption or the absence of sufficient strength.
33357838	4	58	theme	lignocellulose	691:704	arg1	composites					706:715	The lignocellulose composites	687:715	The lignocellulose composites with a millimeter-thick	687:739	The lignocellulose composites with a millimeter-thick mimic the "mortar-brick" layered structure, resulting in excellent mechanical properties that can achieve the compressive strength of 288 MPa and EMI shielding effectiveness of 39.3 dB.
33357838	4	59	theme	"	764:764	arg1	structure					774:782	the "mortar-brick" layered structure	747:782	the "mortar-brick" layered structure	747:782	The lignocellulose composites with a millimeter-thick mimic the "mortar-brick" layered structure, resulting in excellent mechanical properties that can achieve the compressive strength of 288 MPa and EMI shielding effectiveness of 39.3 dB.
33357838	1	60	theme	severe	276:281	arg1	pollution					293:301	increasingly severe radiation pollution	263:301	increasingly severe radiation pollution	263:301	Electromagnetic-interference (EMI) shielding materials that are green, lightweight, and with high mechanical properties need to be urgently developed to address increasingly severe radiation pollution.
33190094	5	0	theme	maximum	751:757	arg1	capacities					770:779	the maximum adsorption capacities	747:779	the maximum adsorption capacities of SMX and SPD	747:794	Equilibrium adsorption studies showed that the maximum adsorption capacities of SMX and SPD decreased from 312.28 to 272.83 mg/g and 161.89 to 146.56 mg/g, respectively, as the temperature increased from 298 to 318 K. Reusability experiments indicated that CMC/SGO-GCC maintained a high adsorption capacity for SMX and SPD upon its reuse.
33190094	0	1	theme	chitosan-based	75:88	arg1	composite					90:98	a novel carboxymethyl cellulose and chitosan-based composite	39:98	a novel carboxymethyl cellulose and chitosan-based composite with sulfonated graphene oxide	39:129	Enhanced adsorption of sulfonamides by a novel carboxymethyl cellulose and chitosan-based composite with sulfonated graphene oxide.
33190094	0	2	with	composite	90:98	arg1	oxide					125:129	sulfonated graphene oxide	105:129	sulfonated graphene oxide	105:129	Enhanced adsorption of sulfonamides by a novel carboxymethyl cellulose and chitosan-based composite with sulfonated graphene oxide.
33190094	0	3	theme	sulfonated	105:114	arg1	oxide					125:129	sulfonated graphene oxide	105:129	sulfonated graphene oxide	105:129	Enhanced adsorption of sulfonamides by a novel carboxymethyl cellulose and chitosan-based composite with sulfonated graphene oxide.
33190094	3	4	theme	high	608:611	arg1	SMX					631:633	SMX	631:633	SMX	631:633	This adsorption process is spontaneous and pH dependent, and shows high sulfamethoxazole (SMX) and sulfapyridine (SPD).
33190094	3	4	theme	high	608:611	arg1	sulfamethoxazole					613:628	high sulfamethoxazole	608:628	high sulfamethoxazole (SMX)	608:634	This adsorption process is spontaneous and pH dependent, and shows high sulfamethoxazole (SMX) and sulfapyridine (SPD).
33190094	5	5	theme	adsorption	991:1000	arg1	capacity					1002:1009	a high adsorption capacity	984:1009	a high adsorption capacity for SMX and SPD	984:1025	Equilibrium adsorption studies showed that the maximum adsorption capacities of SMX and SPD decreased from 312.28 to 272.83 mg/g and 161.89 to 146.56 mg/g, respectively, as the temperature increased from 298 to 318 K. Reusability experiments indicated that CMC/SGO-GCC maintained a high adsorption capacity for SMX and SPD upon its reuse.
33190094	5	6	theme	adsorption	716:725	arg1	studies					727:733	Equilibrium adsorption studies	704:733	Equilibrium adsorption studies	704:733	Equilibrium adsorption studies showed that the maximum adsorption capacities of SMX and SPD decreased from 312.28 to 272.83 mg/g and 161.89 to 146.56 mg/g, respectively, as the temperature increased from 298 to 318 K. Reusability experiments indicated that CMC/SGO-GCC maintained a high adsorption capacity for SMX and SPD upon its reuse.
33190094	1	7	theme	adsorbing	352:360	arg1	antibiotics					374:384	adsorbing sulfonamide antibiotics	352:384	adsorbing sulfonamide antibiotics	352:384	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	2	8	theme	CMC	506:508	arg1	CMC					506:508	CMC	506:508	CMC	506:508	The GO contains a variety of carboxyl and hydroxyl groups, which can interact with the hydroxyl groups of chitosan and CMC to form strong hydrogen bonds.
33190094	2	8	theme	CMC	506:508	arg1	chitosan					493:500	chitosan	493:500	chitosan	493:500	The GO contains a variety of carboxyl and hydroxyl groups, which can interact with the hydroxyl groups of chitosan and CMC to form strong hydrogen bonds.
33190094	2	8	theme	CMC	506:508	arg1	groups					483:488	the hydroxyl groups	470:488	the hydroxyl groups of chitosan and CMC	470:508	The GO contains a variety of carboxyl and hydroxyl groups, which can interact with the hydroxyl groups of chitosan and CMC to form strong hydrogen bonds.
33190094	1	9	theme	sulfonamide	362:372	arg1	antibiotics					374:384	adsorbing sulfonamide antibiotics	352:384	adsorbing sulfonamide antibiotics	352:384	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	0	10	theme	graphene	116:123	arg1	oxide					125:129	sulfonated graphene oxide	105:129	sulfonated graphene oxide	105:129	Enhanced adsorption of sulfonamides by a novel carboxymethyl cellulose and chitosan-based composite with sulfonated graphene oxide.
33190094	5	11	dep	experiments	934:944	arg1	to					912:913	to	912:913	to	912:913	Equilibrium adsorption studies showed that the maximum adsorption capacities of SMX and SPD decreased from 312.28 to 272.83 mg/g and 161.89 to 146.56 mg/g, respectively, as the temperature increased from 298 to 318 K. Reusability experiments indicated that CMC/SGO-GCC maintained a high adsorption capacity for SMX and SPD upon its reuse.
33190094	5	12	theme	SPD	792:794	arg1	capacities					770:779	the maximum adsorption capacities	747:779	the maximum adsorption capacities of SMX and SPD	747:794	Equilibrium adsorption studies showed that the maximum adsorption capacities of SMX and SPD decreased from 312.28 to 272.83 mg/g and 161.89 to 146.56 mg/g, respectively, as the temperature increased from 298 to 318 K. Reusability experiments indicated that CMC/SGO-GCC maintained a high adsorption capacity for SMX and SPD upon its reuse.
33190094	2	13	theme	hydroxyl	474:481	arg1	CMC					506:508	CMC	506:508	CMC	506:508	The GO contains a variety of carboxyl and hydroxyl groups, which can interact with the hydroxyl groups of chitosan and CMC to form strong hydrogen bonds.
33190094	2	13	theme	hydroxyl	474:481	arg1	chitosan					493:500	chitosan	493:500	chitosan	493:500	The GO contains a variety of carboxyl and hydroxyl groups, which can interact with the hydroxyl groups of chitosan and CMC to form strong hydrogen bonds.
33190094	2	13	theme	hydroxyl	474:481	arg1	groups					483:488	the hydroxyl groups	470:488	the hydroxyl groups of chitosan and CMC	470:508	The GO contains a variety of carboxyl and hydroxyl groups, which can interact with the hydroxyl groups of chitosan and CMC to form strong hydrogen bonds.
33190094	5	14	theme	318 K.	915:920	arg1	experiments					934:944	298 to 318 K. Reusability experiments	908:944	298 to 318 K. Reusability experiments	908:944	Equilibrium adsorption studies showed that the maximum adsorption capacities of SMX and SPD decreased from 312.28 to 272.83 mg/g and 161.89 to 146.56 mg/g, respectively, as the temperature increased from 298 to 318 K. Reusability experiments indicated that CMC/SGO-GCC maintained a high adsorption capacity for SMX and SPD upon its reuse.
33190094	1	15	theme	carboxymethyl	189:201	arg1	CMC					214:216	CMC	214:216	CMC	214:216	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	1	15	theme	carboxymethyl	189:201	arg1	cellulose					203:211	carboxymethyl cellulose	189:211	carboxymethyl cellulose (CMC)	189:217	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	1	16	theme	sulfonated	285:294	arg1	oxide					305:309	sulfonated graphene oxide	285:309	sulfonated graphene oxide (CMC/SGO-GCC)	285:323	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	1	16	theme	sulfonated	285:294	arg1	CMC/SGO-GCC					312:322	CMC/SGO-GCC	312:322	CMC/SGO-GCC	312:322	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	5	17	theme	Reusability	922:932	arg1	experiments					934:944	298 to 318 K. Reusability experiments	908:944	298 to 318 K. Reusability experiments	908:944	Equilibrium adsorption studies showed that the maximum adsorption capacities of SMX and SPD decreased from 312.28 to 272.83 mg/g and 161.89 to 146.56 mg/g, respectively, as the temperature increased from 298 to 318 K. Reusability experiments indicated that CMC/SGO-GCC maintained a high adsorption capacity for SMX and SPD upon its reuse.
33190094	0	18	theme	sulfonamides	23:34	arg1	adsorption					9:18	adsorption	9:18	adsorption of sulfonamides	9:34	Enhanced adsorption of sulfonamides by a novel carboxymethyl cellulose and chitosan-based composite with sulfonated graphene oxide.
33190094	1	19	theme	graphene	296:303	arg1	oxide					305:309	sulfonated graphene oxide	285:309	sulfonated graphene oxide (CMC/SGO-GCC)	285:323	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	1	19	theme	graphene	296:303	arg1	CMC/SGO-GCC					312:322	CMC/SGO-GCC	312:322	CMC/SGO-GCC	312:322	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	1	20	link	crosslinked	231:241	arg1	carboxyalkyl-chitosan					243:263	genipin crosslinked carboxyalkyl-chitosan	223:263	genipin crosslinked carboxyalkyl-chitosan (GCC)	223:269	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	1	20	link	crosslinked	231:241	arg1	GCC					266:268	GCC	266:268	GCC	266:268	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	5	21	theme	SMX	784:786	arg1	capacities					770:779	the maximum adsorption capacities	747:779	the maximum adsorption capacities of SMX and SPD	747:794	Equilibrium adsorption studies showed that the maximum adsorption capacities of SMX and SPD decreased from 312.28 to 272.83 mg/g and 161.89 to 146.56 mg/g, respectively, as the temperature increased from 298 to 318 K. Reusability experiments indicated that CMC/SGO-GCC maintained a high adsorption capacity for SMX and SPD upon its reuse.
33190094	2	22	theme	hydroxyl	429:436	arg1	groups					438:443	carboxyl and hydroxyl groups	416:443	groups	438:443	The GO contains a variety of carboxyl and hydroxyl groups, which can interact with the hydroxyl groups of chitosan and CMC to form strong hydrogen bonds.
33190094	3	23	theme	adsorption	546:555	arg1	process					557:563	This adsorption process	541:563	This adsorption process	541:563	This adsorption process is spontaneous and pH dependent, and shows high sulfamethoxazole (SMX) and sulfapyridine (SPD).
33190094	2	24	theme	carboxyl	416:423	arg1	groups					438:443	carboxyl and hydroxyl groups	416:443	groups	438:443	The GO contains a variety of carboxyl and hydroxyl groups, which can interact with the hydroxyl groups of chitosan and CMC to form strong hydrogen bonds.
33190094	2	25	theme	hydrogen	525:532	arg1	bonds					534:538	strong hydrogen bonds	518:538	strong hydrogen bonds	518:538	The GO contains a variety of carboxyl and hydroxyl groups, which can interact with the hydroxyl groups of chitosan and CMC to form strong hydrogen bonds.
33190094	2	26	theme	strong	518:523	arg1	bonds					534:538	strong hydrogen bonds	518:538	strong hydrogen bonds	518:538	The GO contains a variety of carboxyl and hydroxyl groups, which can interact with the hydroxyl groups of chitosan and CMC to form strong hydrogen bonds.
33190094	2	27	contain	contains	394:401	arg1	The					387:389	The	387:389	The	387:389	The GO contains a variety of carboxyl and hydroxyl groups, which can interact with the hydroxyl groups of chitosan and CMC to form strong hydrogen bonds.
33190094	2	27	contain	contains	394:401	arg2	variety					405:411	a variety	403:411	a variety	403:411	The GO contains a variety of carboxyl and hydroxyl groups, which can interact with the hydroxyl groups of chitosan and CMC to form strong hydrogen bonds.
33190094	2	27	contain	contains	394:401	arg2	groups					438:443	carboxyl and hydroxyl groups	416:443	groups	438:443	The GO contains a variety of carboxyl and hydroxyl groups, which can interact with the hydroxyl groups of chitosan and CMC to form strong hydrogen bonds.
33190094	2	28	theme	groups	438:443	arg1	groups					438:443	carboxyl and hydroxyl groups	416:443	groups	438:443	The GO contains a variety of carboxyl and hydroxyl groups, which can interact with the hydroxyl groups of chitosan and CMC to form strong hydrogen bonds.
33190094	2	28	theme	groups	438:443	arg1	variety					405:411	a variety	403:411	a variety	403:411	The GO contains a variety of carboxyl and hydroxyl groups, which can interact with the hydroxyl groups of chitosan and CMC to form strong hydrogen bonds.
33190094	0	29	theme	carboxymethyl	47:59	arg1	composite					90:98	a novel carboxymethyl cellulose and chitosan-based composite	39:98	a novel carboxymethyl cellulose and chitosan-based composite with sulfonated graphene oxide	39:129	Enhanced adsorption of sulfonamides by a novel carboxymethyl cellulose and chitosan-based composite with sulfonated graphene oxide.
33190094	5	30	theme	Equilibrium	704:714	arg1	studies					727:733	Equilibrium adsorption studies	704:733	Equilibrium adsorption studies	704:733	Equilibrium adsorption studies showed that the maximum adsorption capacities of SMX and SPD decreased from 312.28 to 272.83 mg/g and 161.89 to 146.56 mg/g, respectively, as the temperature increased from 298 to 318 K. Reusability experiments indicated that CMC/SGO-GCC maintained a high adsorption capacity for SMX and SPD upon its reuse.
33190094	4	31	theme	aqueous	685:691	arg1	solutions					693:701	aqueous solutions	685:701	aqueous solutions	685:701	Removal efficiency from aqueous solutions.
33190094	0	32	theme	novel	41:45	arg1	composite					90:98	a novel carboxymethyl cellulose and chitosan-based composite	39:98	a novel carboxymethyl cellulose and chitosan-based composite with sulfonated graphene oxide	39:129	Enhanced adsorption of sulfonamides by a novel carboxymethyl cellulose and chitosan-based composite with sulfonated graphene oxide.
33190094	5	33	theme	high	986:989	arg1	capacity					1002:1009	a high adsorption capacity	984:1009	a high adsorption capacity for SMX and SPD	984:1025	Equilibrium adsorption studies showed that the maximum adsorption capacities of SMX and SPD decreased from 312.28 to 272.83 mg/g and 161.89 to 146.56 mg/g, respectively, as the temperature increased from 298 to 318 K. Reusability experiments indicated that CMC/SGO-GCC maintained a high adsorption capacity for SMX and SPD upon its reuse.
33190094	1	34	theme	novel	134:138	arg1	material					339:346	a material	337:346	a material for adsorbing sulfonamide antibiotics	337:384	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	1	34	theme	novel	134:138	arg1	sponge					162:167	A novel multiple active sites sponge	132:167	A novel multiple active sites sponge	132:167	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	4	35	from	solutions	693:701	arg1	efficiency					669:678	efficiency	669:678	efficiency	669:678	Removal efficiency from aqueous solutions.
33190094	1	36	theme	genipin	223:229	arg1	carboxyalkyl-chitosan					243:263	genipin crosslinked carboxyalkyl-chitosan	223:263	genipin crosslinked carboxyalkyl-chitosan (GCC)	223:269	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	1	36	theme	genipin	223:229	arg1	GCC					266:268	GCC	266:268	GCC	266:268	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	2	37	dep	The	387:389	arg1	GO					391:392	GO	391:392	GO	391:392	The GO contains a variety of carboxyl and hydroxyl groups, which can interact with the hydroxyl groups of chitosan and CMC to form strong hydrogen bonds.
33190094	5	38	theme	adsorption	759:768	arg1	capacities					770:779	the maximum adsorption capacities	747:779	the maximum adsorption capacities of SMX and SPD	747:794	Equilibrium adsorption studies showed that the maximum adsorption capacities of SMX and SPD decreased from 312.28 to 272.83 mg/g and 161.89 to 146.56 mg/g, respectively, as the temperature increased from 298 to 318 K. Reusability experiments indicated that CMC/SGO-GCC maintained a high adsorption capacity for SMX and SPD upon its reuse.
33190094	1	39	theme	multiple	140:147	arg1	sites					156:160	multiple active sites	140:160	A novel multiple active sites sponge	132:167	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	1	40	theme	crosslinked	231:241	arg1	carboxyalkyl-chitosan					243:263	genipin crosslinked carboxyalkyl-chitosan	223:263	genipin crosslinked carboxyalkyl-chitosan (GCC)	223:269	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	1	40	theme	crosslinked	231:241	arg1	GCC					266:268	GCC	266:268	GCC	266:268	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	2	41	theme	chitosan	493:500	arg1	CMC					506:508	CMC	506:508	CMC	506:508	The GO contains a variety of carboxyl and hydroxyl groups, which can interact with the hydroxyl groups of chitosan and CMC to form strong hydrogen bonds.
33190094	2	41	theme	chitosan	493:500	arg1	chitosan					493:500	chitosan	493:500	chitosan	493:500	The GO contains a variety of carboxyl and hydroxyl groups, which can interact with the hydroxyl groups of chitosan and CMC to form strong hydrogen bonds.
33190094	2	41	theme	chitosan	493:500	arg1	groups					483:488	the hydroxyl groups	470:488	the hydroxyl groups of chitosan and CMC	470:508	The GO contains a variety of carboxyl and hydroxyl groups, which can interact with the hydroxyl groups of chitosan and CMC to form strong hydrogen bonds.
33190094	6	42	theme	ideal	1083:1087	arg1	material					1089:1096	an ideal material	1080:1096	an ideal material for adsorbing SMX and SPD	1080:1122	This study shows that CMC/SGO-GCC is an ideal material for adsorbing SMX and SPD.
33190094	6	42	theme	ideal	1083:1087	arg1	CMC/SGO-GCC					1065:1075	CMC/SGO-GCC	1065:1075	CMC/SGO-GCC	1065:1075	This study shows that CMC/SGO-GCC is an ideal material for adsorbing SMX and SPD.
33190094	1	43	theme	active	149:154	arg1	sites					156:160	multiple active sites	140:160	A novel multiple active sites sponge	132:167	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	0	44	theme	cellulose	61:69	arg1	composite					90:98	a novel carboxymethyl cellulose and chitosan-based composite	39:98	a novel carboxymethyl cellulose and chitosan-based composite with sulfonated graphene oxide	39:129	Enhanced adsorption of sulfonamides by a novel carboxymethyl cellulose and chitosan-based composite with sulfonated graphene oxide.
33190094	1	45	used	used	329:332	arg2	sponge					162:167	A novel multiple active sites sponge	132:167	A novel multiple active sites sponge	132:167	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	1	45	used	used	329:332	arg2	material					339:346	a material	337:346	a material for adsorbing sulfonamide antibiotics	337:384	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	5	46	dep	272.83 mg/g	821:831	arg1	312.28					811:816	312.28	811:816	312.28	811:816	Equilibrium adsorption studies showed that the maximum adsorption capacities of SMX and SPD decreased from 312.28 to 272.83 mg/g and 161.89 to 146.56 mg/g, respectively, as the temperature increased from 298 to 318 K. Reusability experiments indicated that CMC/SGO-GCC maintained a high adsorption capacity for SMX and SPD upon its reuse.
33190094	1	47	theme	sites	156:160	arg1	material					339:346	a material	337:346	a material for adsorbing sulfonamide antibiotics	337:384	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
33190094	1	47	theme	sites	156:160	arg1	sponge					162:167	A novel multiple active sites sponge	132:167	A novel multiple active sites sponge	132:167	A novel multiple active sites sponge was fabricated from carboxymethyl cellulose (CMC) and genipin crosslinked carboxyalkyl-chitosan (GCC) combined with sulfonated graphene oxide (CMC/SGO-GCC) and used as a material for adsorbing sulfonamide antibiotics.
34432419	6	0	theme	self-cleaning	1048:1060	arg1	abilities					1088:1096	The self-cleaning and liquid-food-repelling abilities	1044:1096	The self-cleaning and liquid-food-repelling abilities of this film	1044:1109	The self-cleaning and liquid-food-repelling abilities of this film were comprehensively confirmed.
34432419	5	1	theme	mechanical	975:984	arg1	properties					986:995	mechanical properties	975:995	mechanical properties of this film	975:1008	The anti-wettability, optical barrier, and mechanical properties of this film were also significantly improved.
34432419	7	2	theme	colorimetric	1177:1188	arg1	film					1197:1200	this super anti-wetting colorimetric starch film	1153:1200	this super anti-wetting colorimetric starch film	1153:1200	Moreover, this super anti-wetting colorimetric starch film can be applied to monitor the freshness of aquatic products without being disabled by water.
34432419	7	3	theme	starch	1190:1195	arg1	film					1197:1200	this super anti-wetting colorimetric starch film	1153:1200	this super anti-wetting colorimetric starch film	1153:1200	Moreover, this super anti-wetting colorimetric starch film can be applied to monitor the freshness of aquatic products without being disabled by water.
34432419	2	4	theme	starch	393:398	arg1	film					400:403	a super anti-wetting colorimetric starch film	359:403	a super anti-wetting colorimetric starch film	359:403	Herein, a super anti-wetting colorimetric starch film was prepared by surface modification with a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating.
34432419	6	5	theme	liquid-food-repelling	1066:1086	arg1	abilities					1088:1096	The self-cleaning and liquid-food-repelling abilities	1044:1096	The self-cleaning and liquid-food-repelling abilities of this film	1044:1109	The self-cleaning and liquid-food-repelling abilities of this film were comprehensively confirmed.
34432419	2	6	with	modification	429:440	arg1	coating					501:507	a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating	447:507	a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating	447:507	Herein, a super anti-wetting colorimetric starch film was prepared by surface modification with a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating.
34432419	5	7	theme	optical	954:960	arg1	barrier					962:968	optical barrier	954:968	optical barrier	954:968	The anti-wettability, optical barrier, and mechanical properties of this film were also significantly improved.
34432419	4	8	theme	sliding	778:784	arg1	angle					786:790	low sliding angle	774:790	low sliding angle (8.15°)	774:798	The obtained film exhibited an extremely high water contact angle (152.46°) and low sliding angle (8.15°) owing to the hierarchical micro-/nanostructure formed by nano-starch aggregates combined with the low-surface-energy PDMS covering.
34432419	4	8	theme	sliding	778:784	arg1	8.15°					793:797	8.15°	793:797	8.15°	793:797	The obtained film exhibited an extremely high water contact angle (152.46°) and low sliding angle (8.15°) owing to the hierarchical micro-/nanostructure formed by nano-starch aggregates combined with the low-surface-energy PDMS covering.
34432419	1	9	theme	practical	307:315	arg1	application					317:327	its practical application	303:327	its practical application in the food industry	303:348	Colorimetric starch film containing anthocyanins is extensively used in eco-friendly intelligent food packaging, but its high water wettability limits its practical application in the food industry.
34432419	0	10	theme	Freshness	130:138	arg1	Monitoring					140:149	Aquatic-Product Freshness Monitoring	114:149	Aquatic-Product Freshness Monitoring	114:149	Super Anti-Wetting Colorimetric Starch-Based Film Modified with Poly(dimethylsiloxane) and Micro-/Nano-Starch for Aquatic-Product Freshness Monitoring.
34432419	0	11	theme	Aquatic-Product	114:128	arg1	Monitoring					140:149	Aquatic-Product Freshness Monitoring	114:149	Aquatic-Product Freshness Monitoring	114:149	Super Anti-Wetting Colorimetric Starch-Based Film Modified with Poly(dimethylsiloxane) and Micro-/Nano-Starch for Aquatic-Product Freshness Monitoring.
34432419	1	12	contain	containing	177:186	arg2	anthocyanins					188:199	anthocyanins	188:199	anthocyanins	188:199	Colorimetric starch film containing anthocyanins is extensively used in eco-friendly intelligent food packaging, but its high water wettability limits its practical application in the food industry.
34432419	1	12	contain	containing	177:186	arg1	film					172:175	Colorimetric starch film	152:175	Colorimetric starch film containing anthocyanins	152:199	Colorimetric starch film containing anthocyanins is extensively used in eco-friendly intelligent food packaging, but its high water wettability limits its practical application in the food industry.
34432419	7	13	theme	anti-wetting	1164:1175	arg1	film					1197:1200	this super anti-wetting colorimetric starch film	1153:1200	this super anti-wetting colorimetric starch film	1153:1200	Moreover, this super anti-wetting colorimetric starch film can be applied to monitor the freshness of aquatic products without being disabled by water.
34432419	0	14	theme	Super	0:4	arg1	Film					45:48	Super Anti-Wetting Colorimetric Starch-Based Film	0:48	Super Anti-Wetting Colorimetric Starch-Based Film	0:48	Super Anti-Wetting Colorimetric Starch-Based Film Modified with Poly(dimethylsiloxane) and Micro-/Nano-Starch for Aquatic-Product Freshness Monitoring.
34432419	0	15	theme	Anti-Wetting	6:17	arg1	Film					45:48	Super Anti-Wetting Colorimetric Starch-Based Film	0:48	Super Anti-Wetting Colorimetric Starch-Based Film	0:48	Super Anti-Wetting Colorimetric Starch-Based Film Modified with Poly(dimethylsiloxane) and Micro-/Nano-Starch for Aquatic-Product Freshness Monitoring.
34432419	1	16	from	application	317:327	arg1	industry					341:348	the food industry	332:348	the food industry	332:348	Colorimetric starch film containing anthocyanins is extensively used in eco-friendly intelligent food packaging, but its high water wettability limits its practical application in the food industry.
34432419	3	17	theme	chemical	608:615	arg1	composition					617:627	surface chemical composition	600:627	surface chemical composition	600:627	The water sensitivity, optical properties, mechanical properties, surface morphology, and surface chemical composition of this film were systemically investigated by multiple methods.
34432419	2	18	theme	surface	421:427	arg1	modification					429:440	surface modification	421:440	surface modification with a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating	421:507	Herein, a super anti-wetting colorimetric starch film was prepared by surface modification with a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating.
34432419	2	19	theme	PDMS	485:488	arg1	coating					501:507	a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating	447:507	a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating	447:507	Herein, a super anti-wetting colorimetric starch film was prepared by surface modification with a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating.
34432419	4	20	theme	low-surface-energy	898:915	arg1	PDMS					917:920	the low-surface-energy PDMS	894:920	the low-surface-energy PDMS covering	894:929	The obtained film exhibited an extremely high water contact angle (152.46°) and low sliding angle (8.15°) owing to the hierarchical micro-/nanostructure formed by nano-starch aggregates combined with the low-surface-energy PDMS covering.
34432419	4	21	theme	low	774:776	arg1	angle					786:790	low sliding angle	774:790	low sliding angle (8.15°)	774:798	The obtained film exhibited an extremely high water contact angle (152.46°) and low sliding angle (8.15°) owing to the hierarchical micro-/nanostructure formed by nano-starch aggregates combined with the low-surface-energy PDMS covering.
34432419	4	21	theme	low	774:776	arg1	8.15°					793:797	8.15°	793:797	8.15°	793:797	The obtained film exhibited an extremely high water contact angle (152.46°) and low sliding angle (8.15°) owing to the hierarchical micro-/nanostructure formed by nano-starch aggregates combined with the low-surface-energy PDMS covering.
34432419	1	22	theme	eco-friendly	224:235	arg1	packaging					254:262	eco-friendly intelligent food packaging	224:262	eco-friendly intelligent food packaging	224:262	Colorimetric starch film containing anthocyanins is extensively used in eco-friendly intelligent food packaging, but its high water wettability limits its practical application in the food industry.
34432419	0	23	theme	Starch-Based	32:43	arg1	Film					45:48	Super Anti-Wetting Colorimetric Starch-Based Film	0:48	Super Anti-Wetting Colorimetric Starch-Based Film	0:48	Super Anti-Wetting Colorimetric Starch-Based Film Modified with Poly(dimethylsiloxane) and Micro-/Nano-Starch for Aquatic-Product Freshness Monitoring.
34432419	7	24	theme	super	1158:1162	arg1	film					1197:1200	this super anti-wetting colorimetric starch film	1153:1200	this super anti-wetting colorimetric starch film	1153:1200	Moreover, this super anti-wetting colorimetric starch film can be applied to monitor the freshness of aquatic products without being disabled by water.
34432419	4	25	theme	nano-starch	857:867	arg1	aggregates					869:878	nano-starch aggregates	857:878	nano-starch aggregates combined with the low-surface-energy PDMS covering	857:929	The obtained film exhibited an extremely high water contact angle (152.46°) and low sliding angle (8.15°) owing to the hierarchical micro-/nanostructure formed by nano-starch aggregates combined with the low-surface-energy PDMS covering.
34432419	2	26	theme	dimethylsiloxane	466:481	arg1	coating					501:507	a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating	447:507	a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating	447:507	Herein, a super anti-wetting colorimetric starch film was prepared by surface modification with a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating.
34432419	0	27	theme	Colorimetric	19:30	arg1	Film					45:48	Super Anti-Wetting Colorimetric Starch-Based Film	0:48	Super Anti-Wetting Colorimetric Starch-Based Film	0:48	Super Anti-Wetting Colorimetric Starch-Based Film Modified with Poly(dimethylsiloxane) and Micro-/Nano-Starch for Aquatic-Product Freshness Monitoring.
34432419	5	28	theme	film	1005:1008	arg1	barrier					962:968	optical barrier	954:968	optical barrier	954:968	The anti-wettability, optical barrier, and mechanical properties of this film were also significantly improved.
34432419	5	28	theme	film	1005:1008	arg1	properties					986:995	mechanical properties	975:995	mechanical properties of this film	975:1008	The anti-wettability, optical barrier, and mechanical properties of this film were also significantly improved.
34432419	5	28	theme	film	1005:1008	arg1	anti-wettability					936:951	The anti-wettability	932:951	The anti-wettability	932:951	The anti-wettability, optical barrier, and mechanical properties of this film were also significantly improved.
34432419	3	29	theme	surface	600:606	arg1	composition					617:627	surface chemical composition	600:627	surface chemical composition	600:627	The water sensitivity, optical properties, mechanical properties, surface morphology, and surface chemical composition of this film were systemically investigated by multiple methods.
34432419	1	30	theme	food	249:252	arg1	packaging					254:262	eco-friendly intelligent food packaging	224:262	eco-friendly intelligent food packaging	224:262	Colorimetric starch film containing anthocyanins is extensively used in eco-friendly intelligent food packaging, but its high water wettability limits its practical application in the food industry.
34432419	1	31	theme	food	336:339	arg1	industry					341:348	the food industry	332:348	the food industry	332:348	Colorimetric starch film containing anthocyanins is extensively used in eco-friendly intelligent food packaging, but its high water wettability limits its practical application in the food industry.
34432419	4	32	theme	obtained	698:705	arg1	film					707:710	The obtained film	694:710	The obtained film	694:710	The obtained film exhibited an extremely high water contact angle (152.46°) and low sliding angle (8.15°) owing to the hierarchical micro-/nanostructure formed by nano-starch aggregates combined with the low-surface-energy PDMS covering.
34432419	3	33	theme	film	637:640	arg1	morphology					584:593	surface morphology	576:593	surface morphology	576:593	The water sensitivity, optical properties, mechanical properties, surface morphology, and surface chemical composition of this film were systemically investigated by multiple methods.
34432419	3	33	theme	film	637:640	arg1	composition					617:627	surface chemical composition	600:627	surface chemical composition	600:627	The water sensitivity, optical properties, mechanical properties, surface morphology, and surface chemical composition of this film were systemically investigated by multiple methods.
34432419	3	33	theme	film	637:640	arg1	sensitivity					520:530	The water sensitivity	510:530	The water sensitivity	510:530	The water sensitivity, optical properties, mechanical properties, surface morphology, and surface chemical composition of this film were systemically investigated by multiple methods.
34432419	3	33	theme	film	637:640	arg1	properties					541:550	optical properties	533:550	optical properties	533:550	The water sensitivity, optical properties, mechanical properties, surface morphology, and surface chemical composition of this film were systemically investigated by multiple methods.
34432419	3	33	theme	film	637:640	arg1	properties					564:573	mechanical properties	553:573	mechanical properties	553:573	The water sensitivity, optical properties, mechanical properties, surface morphology, and surface chemical composition of this film were systemically investigated by multiple methods.
34432419	2	34	theme	nano-starch/poly	449:464	arg1	coating					501:507	a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating	447:507	a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating	447:507	Herein, a super anti-wetting colorimetric starch film was prepared by surface modification with a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating.
34432419	4	35	theme	water	740:744	arg1	152.46°					761:767	152.46°	761:767	152.46°	761:767	The obtained film exhibited an extremely high water contact angle (152.46°) and low sliding angle (8.15°) owing to the hierarchical micro-/nanostructure formed by nano-starch aggregates combined with the low-surface-energy PDMS covering.
34432419	4	35	theme	water	740:744	arg1	angle					754:758	an extremely high water contact angle	722:758	an extremely high water contact angle (152.46°)	722:768	The obtained film exhibited an extremely high water contact angle (152.46°) and low sliding angle (8.15°) owing to the hierarchical micro-/nanostructure formed by nano-starch aggregates combined with the low-surface-energy PDMS covering.
34432419	7	36	theme	aquatic	1245:1251	arg1	products					1253:1260	aquatic products	1245:1260	aquatic products	1245:1260	Moreover, this super anti-wetting colorimetric starch film can be applied to monitor the freshness of aquatic products without being disabled by water.
34432419	7	37	theme	products	1253:1260	arg1	freshness					1232:1240	the freshness	1228:1240	the freshness of aquatic products	1228:1260	Moreover, this super anti-wetting colorimetric starch film can be applied to monitor the freshness of aquatic products without being disabled by water.
34432419	2	38	theme	colorimetric	380:391	arg1	film					400:403	a super anti-wetting colorimetric starch film	359:403	a super anti-wetting colorimetric starch film	359:403	Herein, a super anti-wetting colorimetric starch film was prepared by surface modification with a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating.
34432419	2	39	theme	composite	491:499	arg1	coating					501:507	a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating	447:507	a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating	447:507	Herein, a super anti-wetting colorimetric starch film was prepared by surface modification with a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating.
34432419	3	40	theme	water	514:518	arg1	sensitivity					520:530	The water sensitivity	510:530	The water sensitivity	510:530	The water sensitivity, optical properties, mechanical properties, surface morphology, and surface chemical composition of this film were systemically investigated by multiple methods.
34432419	2	41	theme	anti-wetting	367:378	arg1	film					400:403	a super anti-wetting colorimetric starch film	359:403	a super anti-wetting colorimetric starch film	359:403	Herein, a super anti-wetting colorimetric starch film was prepared by surface modification with a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating.
34432419	4	42	theme	hierarchical	813:824	arg1	micro-/nanostructure					826:845	the hierarchical micro-/nanostructure	809:845	the hierarchical micro-/nanostructure formed by nano-starch aggregates combined with the low-surface-energy PDMS covering	809:929	The obtained film exhibited an extremely high water contact angle (152.46°) and low sliding angle (8.15°) owing to the hierarchical micro-/nanostructure formed by nano-starch aggregates combined with the low-surface-energy PDMS covering.
34432419	6	43	theme	film	1106:1109	arg1	abilities					1088:1096	The self-cleaning and liquid-food-repelling abilities	1044:1096	The self-cleaning and liquid-food-repelling abilities of this film	1044:1109	The self-cleaning and liquid-food-repelling abilities of this film were comprehensively confirmed.
34432419	1	44	theme	Colorimetric	152:163	arg1	film					172:175	Colorimetric starch film	152:175	Colorimetric starch film containing anthocyanins	152:199	Colorimetric starch film containing anthocyanins is extensively used in eco-friendly intelligent food packaging, but its high water wettability limits its practical application in the food industry.
34432419	4	45	theme	contact	746:752	arg1	152.46°					761:767	152.46°	761:767	152.46°	761:767	The obtained film exhibited an extremely high water contact angle (152.46°) and low sliding angle (8.15°) owing to the hierarchical micro-/nanostructure formed by nano-starch aggregates combined with the low-surface-energy PDMS covering.
34432419	4	45	theme	contact	746:752	arg1	angle					754:758	an extremely high water contact angle	722:758	an extremely high water contact angle (152.46°)	722:768	The obtained film exhibited an extremely high water contact angle (152.46°) and low sliding angle (8.15°) owing to the hierarchical micro-/nanostructure formed by nano-starch aggregates combined with the low-surface-energy PDMS covering.
34432419	3	46	theme	surface	576:582	arg1	morphology					584:593	surface morphology	576:593	surface morphology	576:593	The water sensitivity, optical properties, mechanical properties, surface morphology, and surface chemical composition of this film were systemically investigated by multiple methods.
34432419	3	47	theme	multiple	676:683	arg1	methods					685:691	multiple methods	676:691	multiple methods	676:691	The water sensitivity, optical properties, mechanical properties, surface morphology, and surface chemical composition of this film were systemically investigated by multiple methods.
34432419	1	48	theme	starch	165:170	arg1	film					172:175	Colorimetric starch film	152:175	Colorimetric starch film containing anthocyanins	152:199	Colorimetric starch film containing anthocyanins is extensively used in eco-friendly intelligent food packaging, but its high water wettability limits its practical application in the food industry.
34432419	2	49	theme	super	361:365	arg1	film					400:403	a super anti-wetting colorimetric starch film	359:403	a super anti-wetting colorimetric starch film	359:403	Herein, a super anti-wetting colorimetric starch film was prepared by surface modification with a nano-starch/poly(dimethylsiloxane) (PDMS) composite coating.
34432419	1	50	used	used	216:219	arg2	film					172:175	Colorimetric starch film	152:175	Colorimetric starch film containing anthocyanins	152:199	Colorimetric starch film containing anthocyanins is extensively used in eco-friendly intelligent food packaging, but its high water wettability limits its practical application in the food industry.
34432419	1	51	theme	intelligent	237:247	arg1	packaging					254:262	eco-friendly intelligent food packaging	224:262	eco-friendly intelligent food packaging	224:262	Colorimetric starch film containing anthocyanins is extensively used in eco-friendly intelligent food packaging, but its high water wettability limits its practical application in the food industry.
34432419	3	52	theme	mechanical	553:562	arg1	properties					564:573	mechanical properties	553:573	mechanical properties	553:573	The water sensitivity, optical properties, mechanical properties, surface morphology, and surface chemical composition of this film were systemically investigated by multiple methods.
34432419	1	53	theme	high	273:276	arg1	wettability					284:294	its high water wettability	269:294	its high water wettability	269:294	Colorimetric starch film containing anthocyanins is extensively used in eco-friendly intelligent food packaging, but its high water wettability limits its practical application in the food industry.
34432419	4	54	theme	high	735:738	arg1	152.46°					761:767	152.46°	761:767	152.46°	761:767	The obtained film exhibited an extremely high water contact angle (152.46°) and low sliding angle (8.15°) owing to the hierarchical micro-/nanostructure formed by nano-starch aggregates combined with the low-surface-energy PDMS covering.
34432419	4	54	theme	high	735:738	arg1	angle					754:758	an extremely high water contact angle	722:758	an extremely high water contact angle (152.46°)	722:768	The obtained film exhibited an extremely high water contact angle (152.46°) and low sliding angle (8.15°) owing to the hierarchical micro-/nanostructure formed by nano-starch aggregates combined with the low-surface-energy PDMS covering.
34432419	3	55	theme	optical	533:539	arg1	properties					541:550	optical properties	533:550	optical properties	533:550	The water sensitivity, optical properties, mechanical properties, surface morphology, and surface chemical composition of this film were systemically investigated by multiple methods.
34432419	1	56	theme	water	278:282	arg1	wettability					284:294	its high water wettability	269:294	its high water wettability	269:294	Colorimetric starch film containing anthocyanins is extensively used in eco-friendly intelligent food packaging, but its high water wettability limits its practical application in the food industry.
32080302	18	0	theme	protein	2567:2573	arg1	content					2575:2581	protein content	2567:2581	protein content	2567:2581	Increased root length, leaf area, shoot length, photosynthetic pigments, protein content, carbohydrate content, fresh and dry biomass were observed, in addition these growth increments may be attributed to the increase of antioxidant activities.
32080302	6	1	theme	odorless	999:1006	arg1	powder					1008:1013	odorless powder	999:1013	odorless powder	999:1013	Purified polysaccharides were light green in color and in a form of odorless powder.
32080302	17	2	theme	Priming	2332:2338	arg1	seeds					2340:2344	Priming seeds	2332:2344	Priming seeds of Triticum vulgare and Phaseolus vulgaris with polysaccharides solutions (3 and 5 mg mL-1)	2332:2436	Priming seeds of Triticum vulgare and Phaseolus vulgaris with polysaccharides solutions (3 and 5 mg mL-1) resulted in significant enhancement of seedling growth.
32080302	11	3	theme	vulgaris	1632:1639	arg1	polysaccharides					1649:1663	Chlorella vulgaris soluble polysaccharides	1622:1663	Chlorella vulgaris soluble polysaccharides	1622:1663	Silver nanoparticles (AgNPs) had been biosynthesized using a solution of Chlorella vulgaris soluble polysaccharides.
32080302	16	4	theme	Candida	2320:2326	arg1	sp					2328:2329	Candida sp	2320:2329	Candida sp	2320:2329	The biosynthesized silver nanoparticles were tested for its antimicrobial activity and have positive effects against Bacillus sp., Erwinia sp., Candida sp.
32080302	16	4	theme	Candida	2320:2326	arg1	sp.					2302:2304	Bacillus sp.	2293:2304	Bacillus sp.	2293:2304	The biosynthesized silver nanoparticles were tested for its antimicrobial activity and have positive effects against Bacillus sp., Erwinia sp., Candida sp.
32080302	13	5	theme	-CH3	1936:1939	arg1	presence					1914:1921	the presence	1910:1921	the presence of carbonyl, -CH3, >CH2, C-H,-OH and -NH functional groups	1910:1980	FT-IR analysis for the biosynthesized AgNPs reported the presence of carbonyl, -CH3, >CH2, C-H,-OH and -NH functional groups.
32080302	3	6	theme	protein	525:531	arg1	content					533:539	total protein content	519:539	total protein content (32.99 ± 2.1 mg g-1 PS)	519:563	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	3	6	theme	protein	525:531	arg1	PS					561:562	32.99 ± 2.1 mg g-1 PS	542:562	32.99 ± 2.1 mg g-1 PS	542:562	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	9	7	theme	temperature	1409:1419	arg1	peaks					1421:1425	crystallization temperature peaks	1393:1425	crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C	1393:1457	Differential scanning calorimetry (DSC) analysis showed the characteristic exothermic transition of Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C.
32080302	4	8	theme	polysaccharides	689:703	arg1	analysis					663:670	infrared spectroscopy (FT-IR) analysis	633:670	infrared spectroscopy (FT-IR) analysis of the extracted polysaccharides	633:703	Fourier Transform infrared spectroscopy (FT-IR) analysis of the extracted polysaccharides showed the presence of N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups.
32080302	4	9	theme	C=O	774:776	arg1	groups					789:794	N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups	728:794	N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups	728:794	Fourier Transform infrared spectroscopy (FT-IR) analysis of the extracted polysaccharides showed the presence of N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups.
32080302	12	10	theme	100 mM	1780:1785	arg1	nitrate					1794:1800	100 mM silver nitrate	1780:1800	100 mM silver nitrate	1780:1800	The pale green color solution of soluble polysaccharides was turned brown when it was incubated for 24 hours with 100 mM silver nitrate in the dark, it showed peak maximum located at 430 nm.
32080302	4	11	theme	spectroscopy	642:653	arg1	analysis					663:670	infrared spectroscopy (FT-IR) analysis	633:670	infrared spectroscopy (FT-IR) analysis of the extracted polysaccharides	633:703	Fourier Transform infrared spectroscopy (FT-IR) analysis of the extracted polysaccharides showed the presence of N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups.
32080302	16	12	theme	silver	2195:2200	arg1	nanoparticles					2202:2214	The biosynthesized silver nanoparticles	2176:2214	The biosynthesized silver nanoparticles	2176:2214	The biosynthesized silver nanoparticles were tested for its antimicrobial activity and have positive effects against Bacillus sp., Erwinia sp., Candida sp.
32080302	5	13	theme	UV-Visible	797:806	arg1	analysis					817:824	UV-Visible spectral analysis	797:824	UV-Visible spectral analysis	797:824	UV-Visible spectral analysis shows the presence of proteins, nucleic acids and chemical groups (ester, carbonyl, carboxyl and amine).
32080302	4	14	theme	groups	789:794	arg1	presence					716:723	the presence	712:723	the presence of N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups	712:794	Fourier Transform infrared spectroscopy (FT-IR) analysis of the extracted polysaccharides showed the presence of N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups.
32080302	2	15	theme	polysaccharides	308:322	arg1	growth					283:288	growth	283:288	growth	283:288	This maximizes its growth and production of polysaccharides (PS).
32080302	2	15	theme	polysaccharides	308:322	arg1	production					294:303	production	294:303	production	294:303	This maximizes its growth and production of polysaccharides (PS).
32080302	1	16	theme	wide	198:201	arg1	range					203:207	a wide range	196:207	a wide range of other microalgae	196:227	Chlorella vulgaris, like a wide range of other microalgae, are able to grow mixotrophically.
32080302	18	17	theme	activities	2728:2737	arg1	increase					2704:2711	the increase	2700:2711	the increase of antioxidant activities	2700:2737	Increased root length, leaf area, shoot length, photosynthetic pigments, protein content, carbohydrate content, fresh and dry biomass were observed, in addition these growth increments may be attributed to the increase of antioxidant activities.
32080302	18	18	theme	leaf	2517:2520	arg1	area					2522:2525	leaf area	2517:2525	leaf area	2517:2525	Increased root length, leaf area, shoot length, photosynthetic pigments, protein content, carbohydrate content, fresh and dry biomass were observed, in addition these growth increments may be attributed to the increase of antioxidant activities.
32080302	15	19	theme	EDX	2130:2132	arg1	analysis					2135:2142	Energy-dispersive X-ray (EDX) analysis	2105:2142	Energy-dispersive X-ray (EDX) analysis	2105:2142	Energy-dispersive X-ray (EDX) analysis showed the dominance of silver.
32080302	8	20	theme	vulgaris	1136:1143	arg1	polysaccharide					1153:1166	Chlorella vulgaris soluble polysaccharide	1126:1166	Chlorella vulgaris soluble polysaccharide	1126:1166	Thermogravimetric analysis demonstrates that Chlorella vulgaris soluble polysaccharide is thermostable until 240°C and degradation occurs in three distinct phases.
32080302	18	21	theme	photosynthetic	2542:2555	arg1	pigments					2557:2564	photosynthetic pigments	2542:2564	photosynthetic pigments	2542:2564	Increased root length, leaf area, shoot length, photosynthetic pigments, protein content, carbohydrate content, fresh and dry biomass were observed, in addition these growth increments may be attributed to the increase of antioxidant activities.
32080302	3	22	theme	total	570:574	arg1	carbohydrate					576:587	total carbohydrate	570:587	total carbohydrate (495.44 ± 8.4 mg g-1 PS)	570:612	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	3	22	theme	total	570:574	arg1	PS					610:611	495.44 ± 8.4 mg g-1 PS	590:611	495.44 ± 8.4 mg g-1 PS	590:611	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	1	23	theme	microalgae	218:227	arg1	range					203:207	a wide range	196:207	a wide range of other microalgae	196:227	Chlorella vulgaris, like a wide range of other microalgae, are able to grow mixotrophically.
32080302	9	24	dep	Chlorella	1345:1353	arg1	vulgaris					1355:1362	vulgaris	1355:1362	vulgaris	1355:1362	Differential scanning calorimetry (DSC) analysis showed the characteristic exothermic transition of Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C.
32080302	14	25	contain	have	2045:2048	arg2	shape					2060:2064	spherical shape	2050:2064	spherical shape	2050:2064	Scanning and transmission electron microscopy show that AgNPs have spherical shape with an average particle size of 5.76.
32080302	14	25	contain	have	2045:2048	arg1	AgNPs					2039:2043	AgNPs	2039:2043	AgNPs	2039:2043	Scanning and transmission electron microscopy show that AgNPs have spherical shape with an average particle size of 5.76.
32080302	8	26	theme	distinct	1228:1235	arg1	phases					1237:1242	three distinct phases	1222:1242	three distinct phases	1222:1242	Thermogravimetric analysis demonstrates that Chlorella vulgaris soluble polysaccharide is thermostable until 240°C and degradation occurs in three distinct phases.
32080302	0	27	theme	growth	163:168	arg1	biostimulation					139:152	biostimulation	139:152	biostimulation	139:152	Production, extraction and characterization of Chlorella vulgaris soluble polysaccharides and their applications in AgNPs biosynthesis and biostimulation of plant growth.
32080302	0	27	theme	growth	163:168	arg1	biosynthesis					122:133	AgNPs biosynthesis	116:133	AgNPs biosynthesis	116:133	Production, extraction and characterization of Chlorella vulgaris soluble polysaccharides and their applications in AgNPs biosynthesis and biostimulation of plant growth.
32080302	13	28	theme	C-H	1948:1950	arg1	presence					1914:1921	the presence	1910:1921	the presence of carbonyl, -CH3, >CH2, C-H,-OH and -NH functional groups	1910:1980	FT-IR analysis for the biosynthesized AgNPs reported the presence of carbonyl, -CH3, >CH2, C-H,-OH and -NH functional groups.
32080302	16	29	theme	positive	2268:2275	arg1	effects					2277:2283	positive effects	2268:2283	positive effects	2268:2283	The biosynthesized silver nanoparticles were tested for its antimicrobial activity and have positive effects against Bacillus sp., Erwinia sp., Candida sp.
32080302	5	30	theme	acids	866:870	arg1	presence					836:843	the presence	832:843	the presence of proteins, nucleic acids and chemical groups (ester, carbonyl, carboxyl and amine)	832:928	UV-Visible spectral analysis shows the presence of proteins, nucleic acids and chemical groups (ester, carbonyl, carboxyl and amine).
32080302	5	31	dep	groups	885:890	arg1	amine					923:927	amine	923:927	amine	923:927	UV-Visible spectral analysis shows the presence of proteins, nucleic acids and chemical groups (ester, carbonyl, carboxyl and amine).
32080302	5	31	dep	groups	885:890	arg1	groups					885:890	chemical groups	876:890	chemical groups (ester, carbonyl, carboxyl and amine)	876:928	UV-Visible spectral analysis shows the presence of proteins, nucleic acids and chemical groups (ester, carbonyl, carboxyl and amine).
32080302	5	31	dep	groups	885:890	arg1	carboxyl					910:917	carboxyl	910:917	carboxyl	910:917	UV-Visible spectral analysis shows the presence of proteins, nucleic acids and chemical groups (ester, carbonyl, carboxyl and amine).
32080302	5	31	dep	groups	885:890	arg1	carbonyl					900:907	carbonyl	900:907	carbonyl	900:907	UV-Visible spectral analysis shows the presence of proteins, nucleic acids and chemical groups (ester, carbonyl, carboxyl and amine).
32080302	5	31	dep	groups	885:890	arg1	ester					893:897	ester	893:897	ester	893:897	UV-Visible spectral analysis shows the presence of proteins, nucleic acids and chemical groups (ester, carbonyl, carboxyl and amine).
32080302	13	32	theme	carbonyl	1926:1933	arg1	presence					1914:1921	the presence	1910:1921	the presence of carbonyl, -CH3, >CH2, C-H,-OH and -NH functional groups	1910:1980	FT-IR analysis for the biosynthesized AgNPs reported the presence of carbonyl, -CH3, >CH2, C-H,-OH and -NH functional groups.
32080302	0	33	theme	soluble	66:72	arg1	polysaccharides					74:88	Chlorella vulgaris soluble polysaccharides	47:88	Chlorella vulgaris soluble polysaccharides	47:88	Production, extraction and characterization of Chlorella vulgaris soluble polysaccharides and their applications in AgNPs biosynthesis and biostimulation of plant growth.
32080302	0	34	from	extraction	12:21	arg1	biostimulation					139:152	biostimulation	139:152	biostimulation	139:152	Production, extraction and characterization of Chlorella vulgaris soluble polysaccharides and their applications in AgNPs biosynthesis and biostimulation of plant growth.
32080302	0	34	from	extraction	12:21	arg1	biosynthesis					122:133	AgNPs biosynthesis	116:133	AgNPs biosynthesis	116:133	Production, extraction and characterization of Chlorella vulgaris soluble polysaccharides and their applications in AgNPs biosynthesis and biostimulation of plant growth.
32080302	13	35	theme	biosynthesized	1880:1893	arg1	AgNPs					1895:1899	the biosynthesized AgNPs	1876:1899	the biosynthesized AgNPs	1876:1899	FT-IR analysis for the biosynthesized AgNPs reported the presence of carbonyl, -CH3, >CH2, C-H,-OH and -NH functional groups.
32080302	12	36	theme	polysaccharides	1707:1721	arg1	solution					1687:1694	The pale green color solution	1666:1694	The pale green color solution of soluble polysaccharides	1666:1721	The pale green color solution of soluble polysaccharides was turned brown when it was incubated for 24 hours with 100 mM silver nitrate in the dark, it showed peak maximum located at 430 nm.
32080302	0	37	from	Production	0:9	arg1	biostimulation					139:152	biostimulation	139:152	biostimulation	139:152	Production, extraction and characterization of Chlorella vulgaris soluble polysaccharides and their applications in AgNPs biosynthesis and biostimulation of plant growth.
32080302	0	37	from	Production	0:9	arg1	biosynthesis					122:133	AgNPs biosynthesis	116:133	AgNPs biosynthesis	116:133	Production, extraction and characterization of Chlorella vulgaris soluble polysaccharides and their applications in AgNPs biosynthesis and biostimulation of plant growth.
32080302	3	38	dep	composition	390:400	arg1	fructose					403:410	fructose	403:410	fructose	403:410	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	12	39	dep	24 hours	1766:1773	arg1	showed					1818:1823	showed	1818:1823	showed peak maximum located at 430 nm	1818:1854	The pale green color solution of soluble polysaccharides was turned brown when it was incubated for 24 hours with 100 mM silver nitrate in the dark, it showed peak maximum located at 430 nm.
32080302	0	40	theme	applications	100:111	arg1	extraction					12:21	extraction	12:21	extraction	12:21	Production, extraction and characterization of Chlorella vulgaris soluble polysaccharides and their applications in AgNPs biosynthesis and biostimulation of plant growth.
32080302	0	40	theme	applications	100:111	arg1	Production					0:9	Production	0:9	Production	0:9	Production, extraction and characterization of Chlorella vulgaris soluble polysaccharides and their applications in AgNPs biosynthesis and biostimulation of plant growth.
32080302	0	40	theme	applications	100:111	arg1	characterization					27:42	characterization	27:42	characterization	27:42	Production, extraction and characterization of Chlorella vulgaris soluble polysaccharides and their applications in AgNPs biosynthesis and biostimulation of plant growth.
32080302	9	41	theme	Differential	1245:1256	arg1	DSC					1280:1282	DSC	1280:1282	DSC	1280:1282	Differential scanning calorimetry (DSC) analysis showed the characteristic exothermic transition of Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C.
32080302	9	41	theme	Differential	1245:1256	arg1	calorimetry					1267:1277	Differential scanning calorimetry	1245:1277	Differential scanning calorimetry (DSC) analysis	1245:1292	Differential scanning calorimetry (DSC) analysis showed the characteristic exothermic transition of Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C.
32080302	12	42	theme	pale	1670:1673	arg1	solution					1687:1694	The pale green color solution	1666:1694	The pale green color solution of soluble polysaccharides	1666:1721	The pale green color solution of soluble polysaccharides was turned brown when it was incubated for 24 hours with 100 mM silver nitrate in the dark, it showed peak maximum located at 430 nm.
32080302	12	43	with	24 hours	1766:1773	arg1	nitrate					1794:1800	100 mM silver nitrate	1780:1800	100 mM silver nitrate	1780:1800	The pale green color solution of soluble polysaccharides was turned brown when it was incubated for 24 hours with 100 mM silver nitrate in the dark, it showed peak maximum located at 430 nm.
32080302	3	44	theme	extracted	334:342	arg1	polysaccharides					344:358	The extracted polysaccharides	330:358	The extracted polysaccharides	330:358	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	3	45	dep	fructose	403:410	arg1	glucose					434:440	glucose	434:440	glucose	434:440	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	3	45	dep	fructose	403:410	arg1	lactose					422:428	lactose	422:428	lactose	422:428	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	3	45	dep	fructose	403:410	arg1	maltose					413:419	maltose	413:419	maltose	413:419	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	13	46	theme	FT-IR	1857:1861	arg1	analysis					1863:1870	FT-IR analysis	1857:1870	FT-IR analysis for the biosynthesized AgNPs	1857:1899	FT-IR analysis for the biosynthesized AgNPs reported the presence of carbonyl, -CH3, >CH2, C-H,-OH and -NH functional groups.
32080302	0	47	theme	AgNPs	116:120	arg1	biosynthesis					122:133	AgNPs biosynthesis	116:133	AgNPs biosynthesis	116:133	Production, extraction and characterization of Chlorella vulgaris soluble polysaccharides and their applications in AgNPs biosynthesis and biostimulation of plant growth.
32080302	9	48	theme	calorimetry	1267:1277	arg1	analysis					1285:1292	Differential scanning calorimetry (DSC) analysis	1245:1292	Differential scanning calorimetry (DSC) analysis	1245:1292	Differential scanning calorimetry (DSC) analysis showed the characteristic exothermic transition of Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C.
32080302	12	49	theme	color	1681:1685	arg1	solution					1687:1694	The pale green color solution	1666:1694	The pale green color solution of soluble polysaccharides	1666:1721	The pale green color solution of soluble polysaccharides was turned brown when it was incubated for 24 hours with 100 mM silver nitrate in the dark, it showed peak maximum located at 430 nm.
32080302	0	50	from	characterization	27:42	arg1	biostimulation					139:152	biostimulation	139:152	biostimulation	139:152	Production, extraction and characterization of Chlorella vulgaris soluble polysaccharides and their applications in AgNPs biosynthesis and biostimulation of plant growth.
32080302	0	50	from	characterization	27:42	arg1	biosynthesis					122:133	AgNPs biosynthesis	116:133	AgNPs biosynthesis	116:133	Production, extraction and characterization of Chlorella vulgaris soluble polysaccharides and their applications in AgNPs biosynthesis and biostimulation of plant growth.
32080302	18	51	theme	root	2504:2507	arg1	length					2509:2514	Increased root length	2494:2514	Increased root length	2494:2514	Increased root length, leaf area, shoot length, photosynthetic pigments, protein content, carbohydrate content, fresh and dry biomass were observed, in addition these growth increments may be attributed to the increase of antioxidant activities.
32080302	5	52	theme	chemical	876:883	arg1	groups					885:890	chemical groups	876:890	chemical groups (ester, carbonyl, carboxyl and amine)	876:928	UV-Visible spectral analysis shows the presence of proteins, nucleic acids and chemical groups (ester, carbonyl, carboxyl and amine).
32080302	5	52	theme	chemical	876:883	arg1	amine					923:927	amine	923:927	amine	923:927	UV-Visible spectral analysis shows the presence of proteins, nucleic acids and chemical groups (ester, carbonyl, carboxyl and amine).
32080302	5	52	theme	chemical	876:883	arg1	carboxyl					910:917	carboxyl	910:917	carboxyl	910:917	UV-Visible spectral analysis shows the presence of proteins, nucleic acids and chemical groups (ester, carbonyl, carboxyl and amine).
32080302	5	52	theme	chemical	876:883	arg1	carbonyl					900:907	carbonyl	900:907	carbonyl	900:907	UV-Visible spectral analysis shows the presence of proteins, nucleic acids and chemical groups (ester, carbonyl, carboxyl and amine).
32080302	5	52	theme	chemical	876:883	arg1	ester					893:897	ester	893:897	ester	893:897	UV-Visible spectral analysis shows the presence of proteins, nucleic acids and chemical groups (ester, carbonyl, carboxyl and amine).
32080302	3	53	theme	complex	367:373	arg1	composition					390:400	a complex monosaccharide composition	365:400	a complex monosaccharide composition (fructose, maltose, lactose and glucose)	365:441	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	16	54	theme	antimicrobial	2236:2248	arg1	activity					2250:2257	its antimicrobial activity	2232:2257	its antimicrobial activity	2232:2257	The biosynthesized silver nanoparticles were tested for its antimicrobial activity and have positive effects against Bacillus sp., Erwinia sp., Candida sp.
32080302	7	55	theme	organic	1063:1069	arg1	solvents					1071:1078	other organic solvents	1057:1078	other organic solvents	1057:1078	It was soluble in water but insoluble in other organic solvents.
32080302	16	56	contain	have	2263:2266	arg2	effects					2277:2283	positive effects	2268:2283	positive effects	2268:2283	The biosynthesized silver nanoparticles were tested for its antimicrobial activity and have positive effects against Bacillus sp., Erwinia sp., Candida sp.
32080302	16	56	contain	have	2263:2266	arg1	nanoparticles					2202:2214	The biosynthesized silver nanoparticles	2176:2214	The biosynthesized silver nanoparticles	2176:2214	The biosynthesized silver nanoparticles were tested for its antimicrobial activity and have positive effects against Bacillus sp., Erwinia sp., Candida sp.
32080302	17	57	theme	vulgaris	2380:2387	arg1	seeds					2340:2344	Priming seeds	2332:2344	Priming seeds of Triticum vulgare and Phaseolus vulgaris with polysaccharides solutions (3 and 5 mg mL-1)	2332:2436	Priming seeds of Triticum vulgare and Phaseolus vulgaris with polysaccharides solutions (3 and 5 mg mL-1) resulted in significant enhancement of seedling growth.
32080302	10	58	theme	X-ray	1464:1468	arg1	diffractogram					1470:1482	The X-ray diffractogram	1460:1482	The X-ray diffractogram	1460:1482	The X-ray diffractogram illustrated the semicrystalline nature of these polysaccharides.
32080302	4	59	dep	Fourier	615:621	arg1	Transform					623:631	Transform	623:631	Transform infrared spectroscopy (FT-IR) analysis of the extracted polysaccharides	623:703	Fourier Transform infrared spectroscopy (FT-IR) analysis of the extracted polysaccharides showed the presence of N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups.
32080302	9	60	from	227.7°C	1451:1457	arg1	peaks					1421:1425	crystallization temperature peaks	1393:1425	crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C	1393:1457	Differential scanning calorimetry (DSC) analysis showed the characteristic exothermic transition of Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C.
32080302	18	61	theme	carbohydrate	2584:2595	arg1	content					2597:2603	carbohydrate content	2584:2603	carbohydrate content	2584:2603	Increased root length, leaf area, shoot length, photosynthetic pigments, protein content, carbohydrate content, fresh and dry biomass were observed, in addition these growth increments may be attributed to the increase of antioxidant activities.
32080302	17	62	theme	polysaccharides	2394:2408	arg1	solutions					2410:2418	polysaccharides solutions	2394:2418	polysaccharides solutions (3 and 5 mg mL-1)	2394:2436	Priming seeds of Triticum vulgare and Phaseolus vulgaris with polysaccharides solutions (3 and 5 mg mL-1) resulted in significant enhancement of seedling growth.
32080302	0	63	theme	Chlorella	47:55	arg1	polysaccharides					74:88	Chlorella vulgaris soluble polysaccharides	47:88	Chlorella vulgaris soluble polysaccharides	47:88	Production, extraction and characterization of Chlorella vulgaris soluble polysaccharides and their applications in AgNPs biosynthesis and biostimulation of plant growth.
32080302	9	64	theme	exothermic	1320:1329	arg1	transition					1331:1340	the characteristic exothermic transition	1301:1340	the characteristic exothermic transition of Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C	1301:1457	Differential scanning calorimetry (DSC) analysis showed the characteristic exothermic transition of Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C.
32080302	9	65	from	162.3°C	1439:1445	arg1	peaks					1421:1425	crystallization temperature peaks	1393:1425	crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C	1393:1457	Differential scanning calorimetry (DSC) analysis showed the characteristic exothermic transition of Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C.
32080302	10	66	theme	semicrystalline	1500:1514	arg1	nature					1516:1521	the semicrystalline nature	1496:1521	the semicrystalline nature of these polysaccharides	1496:1546	The X-ray diffractogram illustrated the semicrystalline nature of these polysaccharides.
32080302	9	67	from	144.1°C	1430:1436	arg1	peaks					1421:1425	crystallization temperature peaks	1393:1425	crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C	1393:1457	Differential scanning calorimetry (DSC) analysis showed the characteristic exothermic transition of Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C.
32080302	17	68	theme	growth	2486:2491	arg1	enhancement					2462:2472	significant enhancement	2450:2472	significant enhancement of seedling growth	2450:2491	Priming seeds of Triticum vulgare and Phaseolus vulgaris with polysaccharides solutions (3 and 5 mg mL-1) resulted in significant enhancement of seedling growth.
32080302	11	69	theme	Chlorella	1622:1630	arg1	polysaccharides					1649:1663	Chlorella vulgaris soluble polysaccharides	1622:1663	Chlorella vulgaris soluble polysaccharides	1622:1663	Silver nanoparticles (AgNPs) had been biosynthesized using a solution of Chlorella vulgaris soluble polysaccharides.
32080302	6	70	theme	powder	1008:1013	arg1	form					991:994	a form	989:994	a form of odorless powder	989:1013	Purified polysaccharides were light green in color and in a form of odorless powder.
32080302	10	71	theme	polysaccharides	1532:1546	arg1	nature					1516:1521	the semicrystalline nature	1496:1521	the semicrystalline nature of these polysaccharides	1496:1546	The X-ray diffractogram illustrated the semicrystalline nature of these polysaccharides.
32080302	18	72	theme	antioxidant	2716:2726	arg1	activities					2728:2737	antioxidant activities	2716:2737	antioxidant activities	2716:2737	Increased root length, leaf area, shoot length, photosynthetic pigments, protein content, carbohydrate content, fresh and dry biomass were observed, in addition these growth increments may be attributed to the increase of antioxidant activities.
32080302	13	73	theme	groups	1975:1980	arg1	presence					1914:1921	the presence	1910:1921	the presence of carbonyl, -CH3, >CH2, C-H,-OH and -NH functional groups	1910:1980	FT-IR analysis for the biosynthesized AgNPs reported the presence of carbonyl, -CH3, >CH2, C-H,-OH and -NH functional groups.
32080302	9	74	theme	polysaccharides	1372:1386	arg1	transition					1331:1340	the characteristic exothermic transition	1301:1340	the characteristic exothermic transition of Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C	1301:1457	Differential scanning calorimetry (DSC) analysis showed the characteristic exothermic transition of Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C.
32080302	18	75	dep	attributed	2686:2695	arg1	addition					2646:2653	addition	2646:2653	addition	2646:2653	Increased root length, leaf area, shoot length, photosynthetic pigments, protein content, carbohydrate content, fresh and dry biomass were observed, in addition these growth increments may be attributed to the increase of antioxidant activities.
32080302	16	76	theme	biosynthesized	2180:2193	arg1	nanoparticles					2202:2214	The biosynthesized silver nanoparticles	2176:2214	The biosynthesized silver nanoparticles	2176:2214	The biosynthesized silver nanoparticles were tested for its antimicrobial activity and have positive effects against Bacillus sp., Erwinia sp., Candida sp.
32080302	11	77	theme	soluble	1641:1647	arg1	polysaccharides					1649:1663	Chlorella vulgaris soluble polysaccharides	1622:1663	Chlorella vulgaris soluble polysaccharides	1622:1663	Silver nanoparticles (AgNPs) had been biosynthesized using a solution of Chlorella vulgaris soluble polysaccharides.
32080302	11	78	theme	Silver	1549:1554	arg1	AgNPs					1571:1575	AgNPs	1571:1575	AgNPs	1571:1575	Silver nanoparticles (AgNPs) had been biosynthesized using a solution of Chlorella vulgaris soluble polysaccharides.
32080302	11	78	theme	Silver	1549:1554	arg1	nanoparticles					1556:1568	Silver nanoparticles	1549:1568	Silver nanoparticles (AgNPs)	1549:1576	Silver nanoparticles (AgNPs) had been biosynthesized using a solution of Chlorella vulgaris soluble polysaccharides.
32080302	3	79	theme	total	519:523	arg1	content					533:539	total protein content	519:539	total protein content (32.99 ± 2.1 mg g-1 PS)	519:563	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	3	79	theme	total	519:523	arg1	PS					561:562	32.99 ± 2.1 mg g-1 PS	542:562	32.99 ± 2.1 mg g-1 PS	542:562	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	9	80	theme	crystallization	1393:1407	arg1	peaks					1421:1425	crystallization temperature peaks	1393:1425	crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C	1393:1457	Differential scanning calorimetry (DSC) analysis showed the characteristic exothermic transition of Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C.
32080302	17	81	dep	solutions	2410:2418	arg1	5 mg mL-1					2427:2435	5 mg mL-1	2427:2435	5 mg mL-1	2427:2435	Priming seeds of Triticum vulgare and Phaseolus vulgaris with polysaccharides solutions (3 and 5 mg mL-1) resulted in significant enhancement of seedling growth.
32080302	17	81	dep	solutions	2410:2418	arg1	3					2421:2421	3	2421:2421	3	2421:2421	Priming seeds of Triticum vulgare and Phaseolus vulgaris with polysaccharides solutions (3 and 5 mg mL-1) resulted in significant enhancement of seedling growth.
32080302	12	82	theme	silver	1787:1792	arg1	nitrate					1794:1800	100 mM silver nitrate	1780:1800	100 mM silver nitrate	1780:1800	The pale green color solution of soluble polysaccharides was turned brown when it was incubated for 24 hours with 100 mM silver nitrate in the dark, it showed peak maximum located at 430 nm.
32080302	17	83	theme	Triticum	2349:2356	arg1	vulgare					2358:2364	Triticum vulgare	2349:2364	Triticum vulgare	2349:2364	Priming seeds of Triticum vulgare and Phaseolus vulgaris with polysaccharides solutions (3 and 5 mg mL-1) resulted in significant enhancement of seedling growth.
32080302	13	84	theme	-OH	1952:1954	arg1	presence					1914:1921	the presence	1910:1921	the presence of carbonyl, -CH3, >CH2, C-H,-OH and -NH functional groups	1910:1980	FT-IR analysis for the biosynthesized AgNPs reported the presence of carbonyl, -CH3, >CH2, C-H,-OH and -NH functional groups.
32080302	4	85	theme	infrared	633:640	arg1	spectroscopy					642:653	infrared spectroscopy	633:653	infrared spectroscopy (FT-IR) analysis of the extracted polysaccharides	633:703	Fourier Transform infrared spectroscopy (FT-IR) analysis of the extracted polysaccharides showed the presence of N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups.
32080302	4	85	theme	infrared	633:640	arg1	FT-IR					656:660	FT-IR	656:660	FT-IR	656:660	Fourier Transform infrared spectroscopy (FT-IR) analysis of the extracted polysaccharides showed the presence of N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups.
32080302	18	86	theme	shoot	2528:2532	arg1	length					2534:2539	shoot length	2528:2539	shoot length	2528:2539	Increased root length, leaf area, shoot length, photosynthetic pigments, protein content, carbohydrate content, fresh and dry biomass were observed, in addition these growth increments may be attributed to the increase of antioxidant activities.
32080302	6	87	from	light	961:965	arg1	color					976:980	color	976:980	color	976:980	Purified polysaccharides were light green in color and in a form of odorless powder.
32080302	6	87	from	light	961:965	arg1	form					991:994	a form	989:994	a form of odorless powder	989:1013	Purified polysaccharides were light green in color and in a form of odorless powder.
32080302	5	88	theme	spectral	808:815	arg1	analysis					817:824	UV-Visible spectral analysis	797:824	UV-Visible spectral analysis	797:824	UV-Visible spectral analysis shows the presence of proteins, nucleic acids and chemical groups (ester, carbonyl, carboxyl and amine).
32080302	8	89	theme	Thermogravimetric	1081:1097	arg1	analysis					1099:1106	Thermogravimetric analysis	1081:1106	Thermogravimetric analysis	1081:1106	Thermogravimetric analysis demonstrates that Chlorella vulgaris soluble polysaccharide is thermostable until 240°C and degradation occurs in three distinct phases.
32080302	4	90	theme	functional	778:787	arg1	groups					789:794	N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups	728:794	N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups	728:794	Fourier Transform infrared spectroscopy (FT-IR) analysis of the extracted polysaccharides showed the presence of N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups.
32080302	13	91	theme	>CH2	1942:1945	arg1	presence					1914:1921	the presence	1910:1921	the presence of carbonyl, -CH3, >CH2, C-H,-OH and -NH functional groups	1910:1980	FT-IR analysis for the biosynthesized AgNPs reported the presence of carbonyl, -CH3, >CH2, C-H,-OH and -NH functional groups.
32080302	12	92	theme	peak	1825:1828	arg1	maximum					1830:1836	peak maximum	1825:1836	peak maximum located at 430 nm	1825:1854	The pale green color solution of soluble polysaccharides was turned brown when it was incubated for 24 hours with 100 mM silver nitrate in the dark, it showed peak maximum located at 430 nm.
32080302	13	93	theme	-NH	1960:1962	arg1	groups					1975:1980	-NH functional groups	1960:1980	-NH functional groups	1960:1980	FT-IR analysis for the biosynthesized AgNPs reported the presence of carbonyl, -CH3, >CH2, C-H,-OH and -NH functional groups.
32080302	17	94	theme	significant	2450:2460	arg1	enhancement					2462:2472	significant enhancement	2450:2472	significant enhancement of seedling growth	2450:2491	Priming seeds of Triticum vulgare and Phaseolus vulgaris with polysaccharides solutions (3 and 5 mg mL-1) resulted in significant enhancement of seedling growth.
32080302	0	95	theme	plant	157:161	arg1	growth					163:168	plant growth	157:168	plant growth	157:168	Production, extraction and characterization of Chlorella vulgaris soluble polysaccharides and their applications in AgNPs biosynthesis and biostimulation of plant growth.
32080302	1	96	theme	other	212:216	arg1	microalgae					218:227	other microalgae	212:227	other microalgae	212:227	Chlorella vulgaris, like a wide range of other microalgae, are able to grow mixotrophically.
32080302	8	97	theme	Chlorella	1126:1134	arg1	polysaccharide					1153:1166	Chlorella vulgaris soluble polysaccharide	1126:1166	Chlorella vulgaris soluble polysaccharide	1126:1166	Thermogravimetric analysis demonstrates that Chlorella vulgaris soluble polysaccharide is thermostable until 240°C and degradation occurs in three distinct phases.
32080302	12	98	from	430 nm	1849:1854	arg1	located					1838:1844	located	1838:1844	located	1838:1844	The pale green color solution of soluble polysaccharides was turned brown when it was incubated for 24 hours with 100 mM silver nitrate in the dark, it showed peak maximum located at 430 nm.
32080302	5	99	theme	proteins	848:855	arg1	presence					836:843	the presence	832:843	the presence of proteins, nucleic acids and chemical groups (ester, carbonyl, carboxyl and amine)	832:928	UV-Visible spectral analysis shows the presence of proteins, nucleic acids and chemical groups (ester, carbonyl, carboxyl and amine).
32080302	8	100	theme	soluble	1145:1151	arg1	polysaccharide					1153:1166	Chlorella vulgaris soluble polysaccharide	1126:1166	Chlorella vulgaris soluble polysaccharide	1126:1166	Thermogravimetric analysis demonstrates that Chlorella vulgaris soluble polysaccharide is thermostable until 240°C and degradation occurs in three distinct phases.
32080302	4	101	theme	extracted	679:687	arg1	polysaccharides					689:703	the extracted polysaccharides	675:703	the extracted polysaccharides	675:703	Fourier Transform infrared spectroscopy (FT-IR) analysis of the extracted polysaccharides showed the presence of N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups.
32080302	5	102	theme	nucleic	858:864	arg1	acids					866:870	nucleic acids	858:870	nucleic acids	858:870	UV-Visible spectral analysis shows the presence of proteins, nucleic acids and chemical groups (ester, carbonyl, carboxyl and amine).
32080302	17	103	with	seeds	2340:2344	arg1	solutions					2410:2418	polysaccharides solutions	2394:2418	polysaccharides solutions (3 and 5 mg mL-1)	2394:2436	Priming seeds of Triticum vulgare and Phaseolus vulgaris with polysaccharides solutions (3 and 5 mg mL-1) resulted in significant enhancement of seedling growth.
32080302	18	104	theme	growth	2661:2666	arg1	increments					2668:2677	these growth increments	2655:2677	these growth increments	2655:2677	Increased root length, leaf area, shoot length, photosynthetic pigments, protein content, carbohydrate content, fresh and dry biomass were observed, in addition these growth increments may be attributed to the increase of antioxidant activities.
32080302	0	105	theme	polysaccharides	74:88	arg1	extraction					12:21	extraction	12:21	extraction	12:21	Production, extraction and characterization of Chlorella vulgaris soluble polysaccharides and their applications in AgNPs biosynthesis and biostimulation of plant growth.
32080302	0	105	theme	polysaccharides	74:88	arg1	Production					0:9	Production	0:9	Production	0:9	Production, extraction and characterization of Chlorella vulgaris soluble polysaccharides and their applications in AgNPs biosynthesis and biostimulation of plant growth.
32080302	0	105	theme	polysaccharides	74:88	arg1	characterization					27:42	characterization	27:42	characterization	27:42	Production, extraction and characterization of Chlorella vulgaris soluble polysaccharides and their applications in AgNPs biosynthesis and biostimulation of plant growth.
32080302	15	106	theme	silver	2168:2173	arg1	dominance					2155:2163	the dominance	2151:2163	the dominance of silver	2151:2173	Energy-dispersive X-ray (EDX) analysis showed the dominance of silver.
32080302	6	107	theme	Purified	931:938	arg1	polysaccharides					940:954	Purified polysaccharides	931:954	Purified polysaccharides	931:954	Purified polysaccharides were light green in color and in a form of odorless powder.
32080302	7	108	from	water	1034:1038	arg1	soluble					1023:1029	soluble	1023:1029	soluble	1023:1029	It was soluble in water but insoluble in other organic solvents.
32080302	7	109	from	insoluble	1044:1052	arg1	solvents					1071:1078	other organic solvents	1057:1078	other organic solvents	1057:1078	It was soluble in water but insoluble in other organic solvents.
32080302	14	110	theme	5.76	2099:2102	arg1	size					2091:2094	an average particle size	2071:2094	an average particle size of 5.76	2071:2102	Scanning and transmission electron microscopy show that AgNPs have spherical shape with an average particle size of 5.76.
32080302	14	111	theme	transmission	1996:2007	arg1	microscopy					2018:2027	Scanning and transmission electron microscopy	1983:2027	microscopy	2018:2027	Scanning and transmission electron microscopy show that AgNPs have spherical shape with an average particle size of 5.76.
32080302	12	112	theme	soluble	1699:1705	arg1	polysaccharides					1707:1721	soluble polysaccharides	1699:1721	soluble polysaccharides	1699:1721	The pale green color solution of soluble polysaccharides was turned brown when it was incubated for 24 hours with 100 mM silver nitrate in the dark, it showed peak maximum located at 430 nm.
32080302	4	113	theme	O-H	733:735	arg1	groups					789:794	N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups	728:794	N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups	728:794	Fourier Transform infrared spectroscopy (FT-IR) analysis of the extracted polysaccharides showed the presence of N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups.
32080302	9	114	theme	scanning	1258:1265	arg1	DSC					1280:1282	DSC	1280:1282	DSC	1280:1282	Differential scanning calorimetry (DSC) analysis showed the characteristic exothermic transition of Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C.
32080302	9	114	theme	scanning	1258:1265	arg1	calorimetry					1267:1277	Differential scanning calorimetry	1245:1277	Differential scanning calorimetry (DSC) analysis	1245:1292	Differential scanning calorimetry (DSC) analysis showed the characteristic exothermic transition of Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C.
32080302	14	115	theme	Scanning	1983:1990	arg1	microscopy					2018:2027	Scanning and transmission electron microscopy	1983:2027	microscopy	2018:2027	Scanning and transmission electron microscopy show that AgNPs have spherical shape with an average particle size of 5.76.
32080302	7	116	from	solvents	1071:1078	arg1	insoluble					1044:1052	insoluble	1044:1052	insoluble	1044:1052	It was soluble in water but insoluble in other organic solvents.
32080302	14	117	theme	average	2074:2080	arg1	size					2091:2094	an average particle size	2071:2094	an average particle size of 5.76	2071:2102	Scanning and transmission electron microscopy show that AgNPs have spherical shape with an average particle size of 5.76.
32080302	4	118	theme	C-H	738:740	arg1	groups					789:794	N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups	728:794	N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups	728:794	Fourier Transform infrared spectroscopy (FT-IR) analysis of the extracted polysaccharides showed the presence of N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups.
32080302	18	119	theme	dry	2616:2618	arg1	biomass					2620:2626	dry biomass	2616:2626	dry biomass	2616:2626	Increased root length, leaf area, shoot length, photosynthetic pigments, protein content, carbohydrate content, fresh and dry biomass were observed, in addition these growth increments may be attributed to the increase of antioxidant activities.
32080302	12	120	theme	green	1675:1679	arg1	solution					1687:1694	The pale green color solution	1666:1694	The pale green color solution of soluble polysaccharides	1666:1721	The pale green color solution of soluble polysaccharides was turned brown when it was incubated for 24 hours with 100 mM silver nitrate in the dark, it showed peak maximum located at 430 nm.
32080302	17	121	theme	vulgare	2358:2364	arg1	seeds					2340:2344	Priming seeds	2332:2344	Priming seeds of Triticum vulgare and Phaseolus vulgaris with polysaccharides solutions (3 and 5 mg mL-1)	2332:2436	Priming seeds of Triticum vulgare and Phaseolus vulgaris with polysaccharides solutions (3 and 5 mg mL-1) resulted in significant enhancement of seedling growth.
32080302	18	122	theme	Increased	2494:2502	arg1	length					2509:2514	Increased root length	2494:2514	Increased root length	2494:2514	Increased root length, leaf area, shoot length, photosynthetic pigments, protein content, carbohydrate content, fresh and dry biomass were observed, in addition these growth increments may be attributed to the increase of antioxidant activities.
32080302	14	123	theme	particle	2082:2089	arg1	size					2091:2094	an average particle size	2071:2094	an average particle size of 5.76	2071:2102	Scanning and transmission electron microscopy show that AgNPs have spherical shape with an average particle size of 5.76.
32080302	7	124	theme	other	1057:1061	arg1	solvents					1071:1078	other organic solvents	1057:1078	other organic solvents	1057:1078	It was soluble in water but insoluble in other organic solvents.
32080302	5	125	theme	groups	885:890	arg1	presence					836:843	the presence	832:843	the presence of proteins, nucleic acids and chemical groups (ester, carbonyl, carboxyl and amine)	832:928	UV-Visible spectral analysis shows the presence of proteins, nucleic acids and chemical groups (ester, carbonyl, carboxyl and amine).
32080302	3	126	theme	monosaccharide	375:388	arg1	composition					390:400	a complex monosaccharide composition	365:400	a complex monosaccharide composition (fructose, maltose, lactose and glucose)	365:441	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	17	127	theme	Phaseolus	2370:2378	arg1	vulgaris					2380:2387	Phaseolus vulgaris	2370:2387	Phaseolus vulgaris	2370:2387	Priming seeds of Triticum vulgare and Phaseolus vulgaris with polysaccharides solutions (3 and 5 mg mL-1) resulted in significant enhancement of seedling growth.
32080302	14	128	theme	spherical	2050:2058	arg1	shape					2060:2064	spherical shape	2050:2064	spherical shape	2050:2064	Scanning and transmission electron microscopy show that AgNPs have spherical shape with an average particle size of 5.76.
32080302	4	129	theme	N-H	728:730	arg1	groups					789:794	N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups	728:794	N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups	728:794	Fourier Transform infrared spectroscopy (FT-IR) analysis of the extracted polysaccharides showed the presence of N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups.
32080302	15	130	theme	Energy-dispersive	2105:2121	arg1	analysis					2135:2142	Energy-dispersive X-ray (EDX) analysis	2105:2142	Energy-dispersive X-ray (EDX) analysis	2105:2142	Energy-dispersive X-ray (EDX) analysis showed the dominance of silver.
32080302	4	131	theme	COO-1	755:759	arg1	groups					789:794	N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups	728:794	N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups	728:794	Fourier Transform infrared spectroscopy (FT-IR) analysis of the extracted polysaccharides showed the presence of N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups.
32080302	0	132	theme	vulgaris	57:64	arg1	polysaccharides					74:88	Chlorella vulgaris soluble polysaccharides	47:88	Chlorella vulgaris soluble polysaccharides	47:88	Production, extraction and characterization of Chlorella vulgaris soluble polysaccharides and their applications in AgNPs biosynthesis and biostimulation of plant growth.
32080302	9	133	theme	characteristic	1305:1318	arg1	transition					1331:1340	the characteristic exothermic transition	1301:1340	the characteristic exothermic transition of Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C	1301:1457	Differential scanning calorimetry (DSC) analysis showed the characteristic exothermic transition of Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C.
32080302	12	134	theme	located	1838:1844	arg1	maximum					1830:1836	peak maximum	1825:1836	peak maximum located at 430 nm	1825:1854	The pale green color solution of soluble polysaccharides was turned brown when it was incubated for 24 hours with 100 mM silver nitrate in the dark, it showed peak maximum located at 430 nm.
32080302	7	135	from	soluble	1023:1029	arg1	water					1034:1038	water	1034:1038	water	1034:1038	It was soluble in water but insoluble in other organic solvents.
32080302	4	136	theme	S=O	762:764	arg1	groups					789:794	N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups	728:794	N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups	728:794	Fourier Transform infrared spectroscopy (FT-IR) analysis of the extracted polysaccharides showed the presence of N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups.
32080302	9	137	with	polysaccharides	1372:1386	arg1	peaks					1421:1425	crystallization temperature peaks	1393:1425	crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C	1393:1457	Differential scanning calorimetry (DSC) analysis showed the characteristic exothermic transition of Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C.
32080302	3	138	theme	uronic	480:485	arg1	acids					487:491	uronic acids	480:491	uronic acids (171.97 ± 5.7 mg g-1 PS)	480:516	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	3	138	theme	uronic	480:485	arg1	PS					514:515	171.97 ± 5.7 mg g-1 PS	494:515	171.97 ± 5.7 mg g-1 PS	494:515	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	3	139	contain	have	360:363	arg2	PS					610:611	495.44 ± 8.4 mg g-1 PS	590:611	495.44 ± 8.4 mg g-1 PS	590:611	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	3	139	contain	have	360:363	arg2	PS					475:476	210.65 ± 10.5 mg g-1 PS	454:476	210.65 ± 10.5 mg g-1 PS	454:476	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	3	139	contain	have	360:363	arg2	sulphate					444:451	sulphate	444:451	sulphate (210.65 ± 10.5 mg g-1 PS)	444:477	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	3	139	contain	have	360:363	arg1	polysaccharides					344:358	The extracted polysaccharides	330:358	The extracted polysaccharides	330:358	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	3	139	contain	have	360:363	arg2	acids					487:491	uronic acids	480:491	uronic acids (171.97 ± 5.7 mg g-1 PS)	480:516	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	3	139	contain	have	360:363	arg2	PS					514:515	171.97 ± 5.7 mg g-1 PS	494:515	171.97 ± 5.7 mg g-1 PS	494:515	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	3	139	contain	have	360:363	arg2	content					533:539	total protein content	519:539	total protein content (32.99 ± 2.1 mg g-1 PS)	519:563	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	3	139	contain	have	360:363	arg2	composition					390:400	a complex monosaccharide composition	365:400	a complex monosaccharide composition (fructose, maltose, lactose and glucose)	365:441	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	3	139	contain	have	360:363	arg2	PS					561:562	32.99 ± 2.1 mg g-1 PS	542:562	32.99 ± 2.1 mg g-1 PS	542:562	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	3	139	contain	have	360:363	arg2	carbohydrate					576:587	total carbohydrate	570:587	total carbohydrate (495.44 ± 8.4 mg g-1 PS)	570:612	The extracted polysaccharides have a complex monosaccharide composition (fructose, maltose, lactose and glucose), sulphate (210.65 ± 10.5 mg g-1 PS), uronic acids (171.97 ± 5.7 mg g-1 PS), total protein content (32.99 ± 2.1 mg g-1 PS), and total carbohydrate (495.44 ± 8.4 mg g-1 PS).
32080302	17	140	theme	seedling	2477:2484	arg1	growth					2486:2491	seedling growth	2477:2491	seedling growth	2477:2491	Priming seeds of Triticum vulgare and Phaseolus vulgaris with polysaccharides solutions (3 and 5 mg mL-1) resulted in significant enhancement of seedling growth.
32080302	14	141	theme	electron	2009:2016	arg1	microscopy					2018:2027	Scanning and transmission electron microscopy	1983:2027	microscopy	2018:2027	Scanning and transmission electron microscopy show that AgNPs have spherical shape with an average particle size of 5.76.
32080302	4	142	theme	-CH3	743:746	arg1	groups					789:794	N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups	728:794	N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups	728:794	Fourier Transform infrared spectroscopy (FT-IR) analysis of the extracted polysaccharides showed the presence of N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups.
32080302	9	143	theme	Chlorella	1345:1353	arg1	polysaccharides					1372:1386	Chlorella vulgaris soluble polysaccharides	1345:1386	Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C	1345:1457	Differential scanning calorimetry (DSC) analysis showed the characteristic exothermic transition of Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C.
32080302	15	144	theme	X-ray	2123:2127	arg1	analysis					2135:2142	Energy-dispersive X-ray (EDX) analysis	2105:2142	Energy-dispersive X-ray (EDX) analysis	2105:2142	Energy-dispersive X-ray (EDX) analysis showed the dominance of silver.
32080302	11	145	theme	polysaccharides	1649:1663	arg1	solution					1610:1617	a solution	1608:1617	a solution of Chlorella vulgaris soluble polysaccharides	1608:1663	Silver nanoparticles (AgNPs) had been biosynthesized using a solution of Chlorella vulgaris soluble polysaccharides.
32080302	4	146	theme	>CH2	749:752	arg1	groups					789:794	N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups	728:794	N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups	728:794	Fourier Transform infrared spectroscopy (FT-IR) analysis of the extracted polysaccharides showed the presence of N-H, O-H, C-H, -CH3, >CH2, COO-1, S=O and the C=O functional groups.
32080302	13	147	theme	functional	1964:1973	arg1	groups					1975:1980	-NH functional groups	1960:1980	-NH functional groups	1960:1980	FT-IR analysis for the biosynthesized AgNPs reported the presence of carbonyl, -CH3, >CH2, C-H,-OH and -NH functional groups.
32080302	9	148	theme	soluble	1364:1370	arg1	polysaccharides					1372:1386	Chlorella vulgaris soluble polysaccharides	1345:1386	Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C	1345:1457	Differential scanning calorimetry (DSC) analysis showed the characteristic exothermic transition of Chlorella vulgaris soluble polysaccharides with crystallization temperature peaks at 144.1°C, 162.3°C and 227.7°C.
32030461	0	0	theme	ultra-basic	74:84	arg1	spring					105:110	an ultra-basic (lignin-stripping) spring	71:110	an ultra-basic (lignin-stripping) spring	71:110	Deconstruction of plant biomass by a Cellulomonas strain isolated from an ultra-basic (lignin-stripping) spring.
32030461	1	1	theme	industrial	301:310	arg1	pretreatment					312:323	the industrial pretreatment	297:323	the industrial pretreatment of lignocellulosic biomass for biofuel production	297:373	Plant material falling into the ultra-basic (pH 11.5-11.9) springs within The Cedars, an actively serpentinizing site in Sonoma County, California, is subject to conditions that mimic the industrial pretreatment of lignocellulosic biomass for biofuel production.
32030461	3	2	theme	synthetic	740:748	arg1	medium					759:764	a synthetic alkaline medium	738:764	a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.)	738:837	We enriched for such bacteria by adding plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.) as the sole source of carbon.
32030461	1	3	theme	ultra-basic	145:155	arg1	springs					172:178	the ultra-basic (pH 11.5-11.9) springs	141:178	the ultra-basic (pH 11.5-11.9) springs within The Cedars	141:196	Plant material falling into the ultra-basic (pH 11.5-11.9) springs within The Cedars, an actively serpentinizing site in Sonoma County, California, is subject to conditions that mimic the industrial pretreatment of lignocellulosic biomass for biofuel production.
32030461	3	4	theme	alkaline	750:757	arg1	medium					759:764	a synthetic alkaline medium	738:764	a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.)	738:837	We enriched for such bacteria by adding plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.) as the sole source of carbon.
32030461	4	5	theme	anaerobic	925:933	arg1	sp					958:959	the facultative anaerobic bacterium Cellulomonas sp	909:959	the facultative anaerobic bacterium Cellulomonas sp	909:959	From the enrichment culture we isolated the facultative anaerobic bacterium Cellulomonas sp.
32030461	2	6	from	springs	451:457	arg1	bacteria					426:433	hemicellulolytic/cellulolytic bacteria	396:433	hemicellulolytic/cellulolytic bacteria from The Cedars springs that are capable of withstanding the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin, making cell wall polysaccharides more accessible to microorganisms and their enzymes	396:660	We sought to obtain hemicellulolytic/cellulolytic bacteria from The Cedars springs that are capable of withstanding the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin, making cell wall polysaccharides more accessible to microorganisms and their enzymes.
32030461	3	7	theme	sole	846:849	arg1	source					851:856	the sole source	842:856	the sole source of carbon	842:866	We enriched for such bacteria by adding plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.) as the sole source of carbon.
32030461	3	7	theme	sole	846:849	arg1	debris					709:714	plant debris	703:714	plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.)	703:837	We enriched for such bacteria by adding plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.) as the sole source of carbon.
32030461	0	8	theme	lignin-stripping	87:102	arg1	spring					105:110	an ultra-basic (lignin-stripping) spring	71:110	an ultra-basic (lignin-stripping) spring	71:110	Deconstruction of plant biomass by a Cellulomonas strain isolated from an ultra-basic (lignin-stripping) spring.
32030461	8	9	theme	near	1481:1484	arg1	activity					1494:1501	near maximal activity	1481:1501	near maximal activity	1481:1501	A strain FA1-encoded β-1, 4-endoxylanase expressed in E. coli was active at a broad pH range, displaying near maximal activity at pH 6-9.
32030461	9	10	theme	alkaline	1575:1582	arg1	springs					1584:1590	extreme alkaline springs	1567:1590	extreme alkaline springs	1567:1590	Discovery of this bacterium illustrates the value of extreme alkaline springs in the search for microorganisms with potential for consolidated bioprocessing of plant biomass to biofuels and other valuable bio-inspired products.
32030461	8	11	theme	broad	1454:1458	arg1	range					1463:1467	a broad pH range	1452:1467	a broad pH range	1452:1467	A strain FA1-encoded β-1, 4-endoxylanase expressed in E. coli was active at a broad pH range, displaying near maximal activity at pH 6-9.
32030461	2	12	theme	calcium	532:538	arg1	lignin					569:574	wherein calcium hydroxide-rich water removes lignin	524:574	the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin	492:574	We sought to obtain hemicellulolytic/cellulolytic bacteria from The Cedars springs that are capable of withstanding the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin, making cell wall polysaccharides more accessible to microorganisms and their enzymes.
32030461	1	13	theme	lignocellulosic	328:342	arg1	biomass					344:350	lignocellulosic biomass	328:350	lignocellulosic biomass	328:350	Plant material falling into the ultra-basic (pH 11.5-11.9) springs within The Cedars, an actively serpentinizing site in Sonoma County, California, is subject to conditions that mimic the industrial pretreatment of lignocellulosic biomass for biofuel production.
32030461	4	14	theme	enrichment	878:887	arg1	culture					889:895	the enrichment culture	874:895	the enrichment culture	874:895	From the enrichment culture we isolated the facultative anaerobic bacterium Cellulomonas sp.
32030461	9	15	with	microorganisms	1610:1623	arg1	potential					1630:1638	potential	1630:1638	potential for consolidated bioprocessing of plant biomass to biofuels and other valuable bio-inspired products	1630:1739	Discovery of this bacterium illustrates the value of extreme alkaline springs in the search for microorganisms with potential for consolidated bioprocessing of plant biomass to biofuels and other valuable bio-inspired products.
32030461	6	16	attach	derived	1253:1259	arg2	it					1246:1247	it	1246:1247	it	1246:1247	Strain FA1 in monoculture colonized the plant material and degraded switchgrass at a faster rate than the community from which it was derived.
32030461	6	16	attach	derived	1253:1259	arg1	community					1225:1233	the community	1221:1233	the community from which it was derived	1221:1259	Strain FA1 in monoculture colonized the plant material and degraded switchgrass at a faster rate than the community from which it was derived.
32030461	2	17	theme	wherein	524:530	arg1	lignin					569:574	wherein calcium hydroxide-rich water removes lignin	524:574	the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin	492:574	We sought to obtain hemicellulolytic/cellulolytic bacteria from The Cedars springs that are capable of withstanding the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin, making cell wall polysaccharides more accessible to microorganisms and their enzymes.
32030461	1	18	theme	biomass	344:350	arg1	pretreatment					312:323	the industrial pretreatment	297:323	the industrial pretreatment of lignocellulosic biomass for biofuel production	297:373	Plant material falling into the ultra-basic (pH 11.5-11.9) springs within The Cedars, an actively serpentinizing site in Sonoma County, California, is subject to conditions that mimic the industrial pretreatment of lignocellulosic biomass for biofuel production.
32030461	9	19	theme	consolidated	1644:1655	arg1	bioprocessing					1657:1669	consolidated bioprocessing	1644:1669	consolidated bioprocessing of plant biomass to biofuels and other valuable bio-inspired products	1644:1739	Discovery of this bacterium illustrates the value of extreme alkaline springs in the search for microorganisms with potential for consolidated bioprocessing of plant biomass to biofuels and other valuable bio-inspired products.
32030461	9	20	from	value	1558:1562	arg1	search					1599:1604	the search	1595:1604	the search for microorganisms with potential for consolidated bioprocessing of plant biomass to biofuels and other valuable bio-inspired products	1595:1739	Discovery of this bacterium illustrates the value of extreme alkaline springs in the search for microorganisms with potential for consolidated bioprocessing of plant biomass to biofuels and other valuable bio-inspired products.
32030461	5	21	dep	polysaccharides	1065:1079	arg1	polysaccharides					1065:1079	the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose	1028:1116	the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose	1028:1116	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	5	21	dep	polysaccharides	1065:1079	arg1	pectin					1092:1097	pectin	1092:1097	pectin	1092:1097	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	5	21	dep	polysaccharides	1065:1079	arg1	hemicellulose					1104:1116	hemicellulose	1104:1116	hemicellulose	1104:1116	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	5	21	dep	polysaccharides	1065:1079	arg1	cellulose					1081:1089	cellulose	1081:1089	cellulose	1081:1089	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	9	22	theme	springs	1584:1590	arg1	value					1558:1562	the value	1554:1562	the value of extreme alkaline springs in the search for microorganisms with potential for consolidated bioprocessing of plant biomass to biofuels and other valuable bio-inspired products	1554:1739	Discovery of this bacterium illustrates the value of extreme alkaline springs in the search for microorganisms with potential for consolidated bioprocessing of plant biomass to biofuels and other valuable bio-inspired products.
32030461	6	23	from	FA1	1126:1128	arg1	monoculture					1133:1143	monoculture	1133:1143	monoculture	1133:1143	Strain FA1 in monoculture colonized the plant material and degraded switchgrass at a faster rate than the community from which it was derived.
32030461	1	24	theme	biofuel	356:362	arg1	production					364:373	biofuel production	356:373	biofuel production	356:373	Plant material falling into the ultra-basic (pH 11.5-11.9) springs within The Cedars, an actively serpentinizing site in Sonoma County, California, is subject to conditions that mimic the industrial pretreatment of lignocellulosic biomass for biofuel production.
32030461	1	25	dep	ultra-basic	145:155	arg1	pH					158:159	pH 11.5-11.9	158:169	pH 11.5-11.9	158:169	Plant material falling into the ultra-basic (pH 11.5-11.9) springs within The Cedars, an actively serpentinizing site in Sonoma County, California, is subject to conditions that mimic the industrial pretreatment of lignocellulosic biomass for biofuel production.
32030461	2	26	theme	wall	589:592	arg1	polysaccharides					594:608	cell wall polysaccharides	584:608	cell wall polysaccharides	584:608	We sought to obtain hemicellulolytic/cellulolytic bacteria from The Cedars springs that are capable of withstanding the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin, making cell wall polysaccharides more accessible to microorganisms and their enzymes.
32030461	2	27	theme	Cedars	444:449	arg1	springs					451:457	The Cedars springs	440:457	The Cedars springs that are capable of withstanding the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin, making cell wall polysaccharides more accessible to microorganisms and their enzymes	440:660	We sought to obtain hemicellulolytic/cellulolytic bacteria from The Cedars springs that are capable of withstanding the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin, making cell wall polysaccharides more accessible to microorganisms and their enzymes.
32030461	2	27	theme	Cedars	444:449	arg1	capable					468:474	capable	468:474	capable	468:474	We sought to obtain hemicellulolytic/cellulolytic bacteria from The Cedars springs that are capable of withstanding the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin, making cell wall polysaccharides more accessible to microorganisms and their enzymes.
32030461	9	28	theme	bacterium	1532:1540	arg1	Discovery					1514:1522	Discovery	1514:1522	Discovery of this bacterium	1514:1540	Discovery of this bacterium illustrates the value of extreme alkaline springs in the search for microorganisms with potential for consolidated bioprocessing of plant biomass to biofuels and other valuable bio-inspired products.
32030461	9	29	theme	plant	1674:1678	arg1	biomass					1680:1686	plant biomass	1674:1686	plant biomass	1674:1686	Discovery of this bacterium illustrates the value of extreme alkaline springs in the search for microorganisms with potential for consolidated bioprocessing of plant biomass to biofuels and other valuable bio-inspired products.
32030461	5	30	theme	strain	962:967	arg1	FA1					969:971	strain FA1	962:971	strain FA1 (NBRC 114238)	962:985	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	5	30	theme	strain	962:967	arg1	NBRC					974:977	NBRC 114238	974:984	NBRC 114238	974:984	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	0	31	theme	biomass	24:30	arg1	Deconstruction					0:13	Deconstruction	0:13	Deconstruction of plant biomass by a Cellulomonas strain isolated from an ultra-basic (lignin-stripping) spring	0:110	Deconstruction of plant biomass by a Cellulomonas strain isolated from an ultra-basic (lignin-stripping) spring.
32030461	7	32	theme	maximal	1336:1342	arg1	concentration					1344:1356	a maximal concentration	1334:1356	a maximal concentration of 13.4% ethanol	1334:1373	Cells of strain FA1 could be acclimated through subculturing to grow at a maximal concentration of 13.4% ethanol.
32030461	4	33	theme	bacterium	935:943	arg1	sp					958:959	the facultative anaerobic bacterium Cellulomonas sp	909:959	the facultative anaerobic bacterium Cellulomonas sp	909:959	From the enrichment culture we isolated the facultative anaerobic bacterium Cellulomonas sp.
32030461	7	34	theme	ethanol	1367:1373	arg1	concentration					1344:1356	a maximal concentration	1334:1356	a maximal concentration of 13.4% ethanol	1334:1373	Cells of strain FA1 could be acclimated through subculturing to grow at a maximal concentration of 13.4% ethanol.
32030461	5	35	theme	plant	1038:1042	arg1	polysaccharides					1065:1079	the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose	1028:1116	the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose	1028:1116	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	5	35	theme	plant	1038:1042	arg1	pectin					1092:1097	pectin	1092:1097	pectin	1092:1097	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	5	35	theme	plant	1038:1042	arg1	hemicellulose					1104:1116	hemicellulose	1104:1116	hemicellulose	1104:1116	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	5	35	theme	plant	1038:1042	arg1	cellulose					1081:1089	cellulose	1081:1089	cellulose	1081:1089	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	8	36	theme	FA1-encoded	1385:1395	arg1	β-1					1397:1399	A strain FA1-encoded β-1	1376:1399	A strain FA1-encoded β-1	1376:1399	A strain FA1-encoded β-1, 4-endoxylanase expressed in E. coli was active at a broad pH range, displaying near maximal activity at pH 6-9.
32030461	8	36	theme	FA1-encoded	1385:1395	arg1	active					1442:1447	active	1442:1447	active	1442:1447	A strain FA1-encoded β-1, 4-endoxylanase expressed in E. coli was active at a broad pH range, displaying near maximal activity at pH 6-9.
32030461	8	36	theme	FA1-encoded	1385:1395	arg1	4-endoxylanase					1402:1415	4-endoxylanase	1402:1415	4-endoxylanase	1402:1415	A strain FA1-encoded β-1, 4-endoxylanase expressed in E. coli was active at a broad pH range, displaying near maximal activity at pH 6-9.
32030461	0	37	theme	plant	18:22	arg1	biomass					24:30	plant biomass	18:30	plant biomass	18:30	Deconstruction of plant biomass by a Cellulomonas strain isolated from an ultra-basic (lignin-stripping) spring.
32030461	2	38	dep	conditions	513:522	arg1	lignin					569:574	wherein calcium hydroxide-rich water removes lignin	524:574	the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin	492:574	We sought to obtain hemicellulolytic/cellulolytic bacteria from The Cedars springs that are capable of withstanding the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin, making cell wall polysaccharides more accessible to microorganisms and their enzymes.
32030461	3	39	theme	for such	675:682	arg1	bacteria					684:691	for such bacteria	675:691	for such bacteria	675:691	We enriched for such bacteria by adding plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.) as the sole source of carbon.
32030461	9	40	theme	bio-inspired	1719:1730	arg1	products					1732:1739	other valuable bio-inspired products	1704:1739	other valuable bio-inspired products	1704:1739	Discovery of this bacterium illustrates the value of extreme alkaline springs in the search for microorganisms with potential for consolidated bioprocessing of plant biomass to biofuels and other valuable bio-inspired products.
32030461	2	41	theme	hemicellulolytic/cellulolytic	396:424	arg1	bacteria					426:433	hemicellulolytic/cellulolytic bacteria	396:433	hemicellulolytic/cellulolytic bacteria from The Cedars springs that are capable of withstanding the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin, making cell wall polysaccharides more accessible to microorganisms and their enzymes	396:660	We sought to obtain hemicellulolytic/cellulolytic bacteria from The Cedars springs that are capable of withstanding the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin, making cell wall polysaccharides more accessible to microorganisms and their enzymes.
32030461	3	42	theme	ground	771:776	arg1	tissue					778:783	ground tissue	771:783	ground tissue of the biofuel crop switchgrass (Panicum virgatum L.)	771:837	We enriched for such bacteria by adding plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.) as the sole source of carbon.
32030461	2	43	theme	removes	561:567	arg1	lignin					569:574	wherein calcium hydroxide-rich water removes lignin	524:574	the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin	492:574	We sought to obtain hemicellulolytic/cellulolytic bacteria from The Cedars springs that are capable of withstanding the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin, making cell wall polysaccharides more accessible to microorganisms and their enzymes.
32030461	3	44	dep	Panicum	818:824	arg1	L.					835:836	Panicum virgatum L.	818:836	Panicum virgatum L.	818:836	We enriched for such bacteria by adding plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.) as the sole source of carbon.
32030461	4	45	theme	Cellulomonas	945:956	arg1	sp					958:959	the facultative anaerobic bacterium Cellulomonas sp	909:959	the facultative anaerobic bacterium Cellulomonas sp	909:959	From the enrichment culture we isolated the facultative anaerobic bacterium Cellulomonas sp.
32030461	9	46	theme	other	1704:1708	arg1	products					1732:1739	other valuable bio-inspired products	1704:1739	other valuable bio-inspired products	1704:1739	Discovery of this bacterium illustrates the value of extreme alkaline springs in the search for microorganisms with potential for consolidated bioprocessing of plant biomass to biofuels and other valuable bio-inspired products.
32030461	8	47	from	pH	1506:1507	arg1	active					1442:1447	active	1442:1447	active	1442:1447	A strain FA1-encoded β-1, 4-endoxylanase expressed in E. coli was active at a broad pH range, displaying near maximal activity at pH 6-9.
32030461	8	47	from	pH	1506:1507	arg1	β-1					1397:1399	A strain FA1-encoded β-1	1376:1399	A strain FA1-encoded β-1	1376:1399	A strain FA1-encoded β-1, 4-endoxylanase expressed in E. coli was active at a broad pH range, displaying near maximal activity at pH 6-9.
32030461	3	48	theme	crop	800:803	arg1	Panicum					818:824	Panicum	818:824	Panicum	818:824	We enriched for such bacteria by adding plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.) as the sole source of carbon.
32030461	3	48	theme	crop	800:803	arg1	switchgrass					805:815	the biofuel crop switchgrass	788:815	the biofuel crop switchgrass (Panicum virgatum L.)	788:837	We enriched for such bacteria by adding plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.) as the sole source of carbon.
32030461	2	49	theme	water	555:559	arg1	lignin					569:574	wherein calcium hydroxide-rich water removes lignin	524:574	the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin	492:574	We sought to obtain hemicellulolytic/cellulolytic bacteria from The Cedars springs that are capable of withstanding the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin, making cell wall polysaccharides more accessible to microorganisms and their enzymes.
32030461	8	50	theme	strain	1378:1383	arg1	β-1					1397:1399	A strain FA1-encoded β-1	1376:1399	A strain FA1-encoded β-1	1376:1399	A strain FA1-encoded β-1, 4-endoxylanase expressed in E. coli was active at a broad pH range, displaying near maximal activity at pH 6-9.
32030461	8	50	theme	strain	1378:1383	arg1	active					1442:1447	active	1442:1447	active	1442:1447	A strain FA1-encoded β-1, 4-endoxylanase expressed in E. coli was active at a broad pH range, displaying near maximal activity at pH 6-9.
32030461	8	50	theme	strain	1378:1383	arg1	4-endoxylanase					1402:1415	4-endoxylanase	1402:1415	4-endoxylanase	1402:1415	A strain FA1-encoded β-1, 4-endoxylanase expressed in E. coli was active at a broad pH range, displaying near maximal activity at pH 6-9.
32030461	0	51	attach	isolated	57:64	arg2	strain					50:55	a Cellulomonas strain	35:55	a Cellulomonas strain isolated from an ultra-basic (lignin-stripping) spring	35:110	Deconstruction of plant biomass by a Cellulomonas strain isolated from an ultra-basic (lignin-stripping) spring.
32030461	0	51	attach	isolated	57:64	arg1	spring					105:110	an ultra-basic (lignin-stripping) spring	71:110	an ultra-basic (lignin-stripping) spring	71:110	Deconstruction of plant biomass by a Cellulomonas strain isolated from an ultra-basic (lignin-stripping) spring.
32030461	3	52	from	springs	725:731	arg1	source					851:856	the sole source	842:856	the sole source of carbon	842:866	We enriched for such bacteria by adding plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.) as the sole source of carbon.
32030461	3	52	from	springs	725:731	arg1	debris					709:714	plant debris	703:714	plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.)	703:837	We enriched for such bacteria by adding plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.) as the sole source of carbon.
32030461	2	53	theme	hydroxide-rich	540:553	arg1	lignin					569:574	wherein calcium hydroxide-rich water removes lignin	524:574	the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin	492:574	We sought to obtain hemicellulolytic/cellulolytic bacteria from The Cedars springs that are capable of withstanding the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin, making cell wall polysaccharides more accessible to microorganisms and their enzymes.
32030461	8	54	from	range	1463:1467	arg1	active					1442:1447	active	1442:1447	active	1442:1447	A strain FA1-encoded β-1, 4-endoxylanase expressed in E. coli was active at a broad pH range, displaying near maximal activity at pH 6-9.
32030461	8	54	from	range	1463:1467	arg1	β-1					1397:1399	A strain FA1-encoded β-1	1376:1399	A strain FA1-encoded β-1	1376:1399	A strain FA1-encoded β-1, 4-endoxylanase expressed in E. coli was active at a broad pH range, displaying near maximal activity at pH 6-9.
32030461	7	55	theme	%	1365:1365	arg1	ethanol					1367:1373	13.4% ethanol	1361:1373	13.4% ethanol	1361:1373	Cells of strain FA1 could be acclimated through subculturing to grow at a maximal concentration of 13.4% ethanol.
32030461	4	56	theme	facultative	913:923	arg1	sp					958:959	the facultative anaerobic bacterium Cellulomonas sp	909:959	the facultative anaerobic bacterium Cellulomonas sp	909:959	From the enrichment culture we isolated the facultative anaerobic bacterium Cellulomonas sp.
32030461	7	57	theme	FA1	1278:1280	arg1	Cells					1262:1266	Cells	1262:1266	Cells of strain FA1	1262:1280	Cells of strain FA1 could be acclimated through subculturing to grow at a maximal concentration of 13.4% ethanol.
32030461	0	58	theme	Cellulomonas	37:48	arg1	strain					50:55	a Cellulomonas strain	35:55	a Cellulomonas strain isolated from an ultra-basic (lignin-stripping) spring	35:110	Deconstruction of plant biomass by a Cellulomonas strain isolated from an ultra-basic (lignin-stripping) spring.
32030461	1	59	from	site	226:229	arg1	California					249:258	California	249:258	California	249:258	Plant material falling into the ultra-basic (pH 11.5-11.9) springs within The Cedars, an actively serpentinizing site in Sonoma County, California, is subject to conditions that mimic the industrial pretreatment of lignocellulosic biomass for biofuel production.
32030461	3	60	theme	plant	703:707	arg1	source					851:856	the sole source	842:856	the sole source of carbon	842:866	We enriched for such bacteria by adding plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.) as the sole source of carbon.
32030461	3	60	theme	plant	703:707	arg1	debris					709:714	plant debris	703:714	plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.)	703:837	We enriched for such bacteria by adding plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.) as the sole source of carbon.
32030461	5	61	theme	major	1032:1036	arg1	polysaccharides					1065:1079	the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose	1028:1116	the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose	1028:1116	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	5	61	theme	major	1032:1036	arg1	pectin					1092:1097	pectin	1092:1097	pectin	1092:1097	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	5	61	theme	major	1032:1036	arg1	hemicellulose					1104:1116	hemicellulose	1104:1116	hemicellulose	1104:1116	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	5	61	theme	major	1032:1036	arg1	cellulose					1081:1089	cellulose	1081:1089	cellulose	1081:1089	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	1	62	theme	Plant	113:117	arg1	material					119:126	Plant material	113:126	Plant material falling into the ultra-basic (pH 11.5-11.9) springs within The Cedars	113:196	Plant material falling into the ultra-basic (pH 11.5-11.9) springs within The Cedars, an actively serpentinizing site in Sonoma County, California, is subject to conditions that mimic the industrial pretreatment of lignocellulosic biomass for biofuel production.
32030461	1	62	theme	Plant	113:117	arg1	site					226:229	an actively serpentinizing site	199:229	an actively serpentinizing site in Sonoma County, California	199:258	Plant material falling into the ultra-basic (pH 11.5-11.9) springs within The Cedars, an actively serpentinizing site in Sonoma County, California, is subject to conditions that mimic the industrial pretreatment of lignocellulosic biomass for biofuel production.
32030461	3	63	theme	carbon	861:866	arg1	source					851:856	the sole source	842:856	the sole source of carbon	842:866	We enriched for such bacteria by adding plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.) as the sole source of carbon.
32030461	3	63	theme	carbon	861:866	arg1	debris					709:714	plant debris	703:714	plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.)	703:837	We enriched for such bacteria by adding plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.) as the sole source of carbon.
32030461	3	64	theme	biofuel	792:798	arg1	Panicum					818:824	Panicum	818:824	Panicum	818:824	We enriched for such bacteria by adding plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.) as the sole source of carbon.
32030461	3	64	theme	biofuel	792:798	arg1	switchgrass					805:815	the biofuel crop switchgrass	788:815	the biofuel crop switchgrass (Panicum virgatum L.)	788:837	We enriched for such bacteria by adding plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.) as the sole source of carbon.
32030461	2	65	theme	cell	584:587	arg1	polysaccharides					594:608	cell wall polysaccharides	584:608	cell wall polysaccharides	584:608	We sought to obtain hemicellulolytic/cellulolytic bacteria from The Cedars springs that are capable of withstanding the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin, making cell wall polysaccharides more accessible to microorganisms and their enzymes.
32030461	5	66	theme	cell	1044:1047	arg1	polysaccharides					1065:1079	the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose	1028:1116	the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose	1028:1116	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	5	66	theme	cell	1044:1047	arg1	pectin					1092:1097	pectin	1092:1097	pectin	1092:1097	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	5	66	theme	cell	1044:1047	arg1	hemicellulose					1104:1116	hemicellulose	1104:1116	hemicellulose	1104:1116	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	5	66	theme	cell	1044:1047	arg1	cellulose					1081:1089	cellulose	1081:1089	cellulose	1081:1089	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	9	67	theme	valuable	1710:1717	arg1	products					1732:1739	other valuable bio-inspired products	1704:1739	other valuable bio-inspired products	1704:1739	Discovery of this bacterium illustrates the value of extreme alkaline springs in the search for microorganisms with potential for consolidated bioprocessing of plant biomass to biofuels and other valuable bio-inspired products.
32030461	9	68	theme	extreme	1567:1573	arg1	springs					1584:1590	extreme alkaline springs	1567:1590	extreme alkaline springs	1567:1590	Discovery of this bacterium illustrates the value of extreme alkaline springs in the search for microorganisms with potential for consolidated bioprocessing of plant biomass to biofuels and other valuable bio-inspired products.
32030461	9	69	theme	biomass	1680:1686	arg1	bioprocessing					1657:1669	consolidated bioprocessing	1644:1669	consolidated bioprocessing of plant biomass to biofuels and other valuable bio-inspired products	1644:1739	Discovery of this bacterium illustrates the value of extreme alkaline springs in the search for microorganisms with potential for consolidated bioprocessing of plant biomass to biofuels and other valuable bio-inspired products.
32030461	6	70	theme	plant	1159:1163	arg1	material					1165:1172	the plant material	1155:1172	the plant material	1155:1172	Strain FA1 in monoculture colonized the plant material and degraded switchgrass at a faster rate than the community from which it was derived.
32030461	5	71	theme	wall-associated	1049:1063	arg1	polysaccharides					1065:1079	the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose	1028:1116	the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose	1028:1116	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	5	71	theme	wall-associated	1049:1063	arg1	pectin					1092:1097	pectin	1092:1097	pectin	1092:1097	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	5	71	theme	wall-associated	1049:1063	arg1	hemicellulose					1104:1116	hemicellulose	1104:1116	hemicellulose	1104:1116	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	5	71	theme	wall-associated	1049:1063	arg1	cellulose					1081:1089	cellulose	1081:1089	cellulose	1081:1089	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	7	72	theme	13.4	1361:1364	arg1	%					1365:1365	%	1365:1365	%	1365:1365	Cells of strain FA1 could be acclimated through subculturing to grow at a maximal concentration of 13.4% ethanol.
32030461	7	73	theme	strain	1271:1276	arg1	FA1					1278:1280	strain FA1	1271:1280	strain FA1	1271:1280	Cells of strain FA1 could be acclimated through subculturing to grow at a maximal concentration of 13.4% ethanol.
32030461	2	74	theme	alkaline	504:511	arg1	conditions					513:522	the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin	492:574	the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin	492:574	We sought to obtain hemicellulolytic/cellulolytic bacteria from The Cedars springs that are capable of withstanding the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin, making cell wall polysaccharides more accessible to microorganisms and their enzymes.
32030461	8	75	theme	pH	1460:1461	arg1	range					1463:1467	a broad pH range	1452:1467	a broad pH range	1452:1467	A strain FA1-encoded β-1, 4-endoxylanase expressed in E. coli was active at a broad pH range, displaying near maximal activity at pH 6-9.
32030461	5	76	theme	high	1004:1007	arg1	pH					1009:1010	high pH	1004:1010	high pH	1004:1010	strain FA1 (NBRC 114238), which tolerates high pH and catabolizes the major plant cell wall-associated polysaccharides cellulose, pectin, and hemicellulose.
32030461	6	77	theme	Strain	1119:1124	arg1	FA1					1126:1128	Strain FA1	1119:1128	Strain FA1 in monoculture	1119:1143	Strain FA1 in monoculture colonized the plant material and degraded switchgrass at a faster rate than the community from which it was derived.
32030461	3	78	with	medium	759:764	arg1	tissue					778:783	ground tissue	771:783	ground tissue of the biofuel crop switchgrass (Panicum virgatum L.)	771:837	We enriched for such bacteria by adding plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.) as the sole source of carbon.
32030461	1	79	theme	serpentinizing	211:224	arg1	material					119:126	Plant material	113:126	Plant material falling into the ultra-basic (pH 11.5-11.9) springs within The Cedars	113:196	Plant material falling into the ultra-basic (pH 11.5-11.9) springs within The Cedars, an actively serpentinizing site in Sonoma County, California, is subject to conditions that mimic the industrial pretreatment of lignocellulosic biomass for biofuel production.
32030461	1	79	theme	serpentinizing	211:224	arg1	site					226:229	an actively serpentinizing site	199:229	an actively serpentinizing site in Sonoma County, California	199:258	Plant material falling into the ultra-basic (pH 11.5-11.9) springs within The Cedars, an actively serpentinizing site in Sonoma County, California, is subject to conditions that mimic the industrial pretreatment of lignocellulosic biomass for biofuel production.
32030461	2	80	theme	extreme	496:502	arg1	conditions					513:522	the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin	492:574	the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin	492:574	We sought to obtain hemicellulolytic/cellulolytic bacteria from The Cedars springs that are capable of withstanding the extreme alkaline conditions wherein calcium hydroxide-rich water removes lignin, making cell wall polysaccharides more accessible to microorganisms and their enzymes.
32030461	6	81	theme	faster	1204:1209	arg1	rate					1211:1214	a faster rate	1202:1214	a faster rate than the community from which it was derived	1202:1259	Strain FA1 in monoculture colonized the plant material and degraded switchgrass at a faster rate than the community from which it was derived.
32030461	8	82	theme	maximal	1486:1492	arg1	activity					1494:1501	near maximal activity	1481:1501	near maximal activity	1481:1501	A strain FA1-encoded β-1, 4-endoxylanase expressed in E. coli was active at a broad pH range, displaying near maximal activity at pH 6-9.
32030461	3	83	theme	switchgrass	805:815	arg1	tissue					778:783	ground tissue	771:783	ground tissue of the biofuel crop switchgrass (Panicum virgatum L.)	771:837	We enriched for such bacteria by adding plant debris from the springs into a synthetic alkaline medium with ground tissue of the biofuel crop switchgrass (Panicum virgatum L.) as the sole source of carbon.
33262077	5	0	theme	phosphate-carbonate	1474:1492	arg1	minerals					1494:1501	phosphate-carbonate minerals	1474:1501	phosphate-carbonate minerals	1474:1501	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	4	1	theme	porous	1103:1108	arg1	structure					1126:1134	a porous and anisotropic structure	1101:1134	a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold	1101:1231	The claw presents a bulk Mg-CaCO3 structure reinforced with fluorapatite coating, while the carotenoid-rich telson presents a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold.
33262077	1	2	theme	Mantis	168:173	arg1	armors					218:223	armors	218:223	armors	218:223	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	1	2	theme	Mantis	168:173	arg1	spears					207:212	spears	207:212	spears	207:212	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	1	2	theme	Mantis	168:173	arg1	cuticle					130:136	The mineralized cuticle	114:136	The mineralized cuticle	114:136	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	1	2	theme	Mantis	168:173	arg1	hammers					198:204	natural hammers	190:204	natural hammers	190:204	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	2	3	theme	added-value	604:614	arg1	nature					544:549	nature	544:549	nature	544:549	Here we aimed to valorize the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before.
33262077	2	3	theme	added-value	604:614	arg1	by-products					616:626	potentially re-usable for novel, smart materials or added-value by-products	552:626	potentially re-usable for novel, smart materials or added-value by-products	552:626	Here we aimed to valorize the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before.
33262077	4	4	theme	amorphous	1142:1150	arg1	mixture					1152:1158	an amorphous mixture	1139:1158	an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold	1139:1231	The claw presents a bulk Mg-CaCO3 structure reinforced with fluorapatite coating, while the carotenoid-rich telson presents a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold.
33262077	1	5	theme	natural	190:196	arg1	cuticle					130:136	The mineralized cuticle	114:136	The mineralized cuticle	114:136	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	1	5	theme	natural	190:196	arg1	hammers					198:204	natural hammers	190:204	natural hammers	190:204	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	1	6	theme	view	383:386	arg1	points					373:378	evolutionary and ecological points	345:378	evolutionary and ecological points of view	345:386	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	3	7	theme	high-resolution	765:779	arg1	microscopy					790:799	high-resolution electron microscopy	765:799	high-resolution electron microscopy	765:799	Employing multi-laser Raman spectroscopy and imaging, supported by x-ray diffraction and high-resolution electron microscopy, we discover that the peripheral segments anatomically known as claws and telson, featured completely different composition and morphology, suggesting different applicability.
33262077	6	8	from	approach	1651:1658	arg1	bio-template					1668:1679	this bio-template	1663:1679	this bio-template	1663:1679	Knowledge-based approach on this bio-template is the basis for smart recycling of such fishery waste for sustainable development, by opening channels for blue bioeconomy avenue.
33262077	6	9	theme	Knowledge-based	1635:1649	arg1	basis					1688:1692	the basis	1684:1692	the basis for smart recycling of such fishery waste for sustainable development	1684:1762	Knowledge-based approach on this bio-template is the basis for smart recycling of such fishery waste for sustainable development, by opening channels for blue bioeconomy avenue.
33262077	6	9	theme	Knowledge-based	1635:1649	arg1	approach					1651:1658	Knowledge-based approach	1635:1658	Knowledge-based approach on this bio-template	1635:1679	Knowledge-based approach on this bio-template is the basis for smart recycling of such fishery waste for sustainable development, by opening channels for blue bioeconomy avenue.
33262077	4	10	from	structure	1126:1134	arg1	deposition					1191:1200	gradient deposition	1182:1200	gradient deposition on the chitin-protein scaffold	1182:1231	The claw presents a bulk Mg-CaCO3 structure reinforced with fluorapatite coating, while the carotenoid-rich telson presents a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold.
33262077	4	11	theme	Mg-CaCO3	1002:1009	arg1	structure					1011:1019	a bulk Mg-CaCO3 structure	995:1019	a bulk Mg-CaCO3 structure reinforced with fluorapatite coating	995:1056	The claw presents a bulk Mg-CaCO3 structure reinforced with fluorapatite coating, while the carotenoid-rich telson presents a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold.
33262077	6	12	theme	fishery	1722:1728	arg1	waste					1730:1734	such fishery waste	1717:1734	such fishery waste	1717:1734	Knowledge-based approach on this bio-template is the basis for smart recycling of such fishery waste for sustainable development, by opening channels for blue bioeconomy avenue.
33262077	5	13	theme	concentrated	1270:1281	arg1	pools					1283:1287	concentrated pools	1270:1287	concentrated pools of astaxanthin carotenoid	1270:1313	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	5	14	theme	adsorbent	1543:1551	arg1	source					1464:1469	a natural source	1454:1469	a natural source of phosphate-carbonate minerals	1454:1501	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	5	14	theme	adsorbent	1543:1551	arg1	pollutant					1533:1541	pollutant adsorbent	1533:1551	pollutant adsorbent	1533:1551	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	5	15	theme	bright	1326:1331	arg1	spots					1337:1341	the bright red spots	1322:1341	the bright red spots visible on telson	1322:1359	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	0	16	theme	mantis	66:71	arg1	shrimp					73:78	the mantis shrimp	62:78	the mantis shrimp	62:78	Promoting hidden natural design templates in wasted shells of the mantis shrimp into valuable biogenic composite.
33262077	2	17	dep	shrimp	438:443	arg1	waste					451:455	shell waste	445:455	the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before	415:673	Here we aimed to valorize the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before.
33262077	6	18	theme	such	1717:1720	arg1	waste					1730:1734	such fishery waste	1717:1734	such fishery waste	1717:1734	Knowledge-based approach on this bio-template is the basis for smart recycling of such fishery waste for sustainable development, by opening channels for blue bioeconomy avenue.
33262077	1	19	theme	shrimps	152:158	arg1	Mantis					168:173	the mantis shrimps Squilla Mantis	141:173	the mantis shrimps Squilla Mantis	141:173	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	2	20	theme	biogenic	512:519	arg1	composite					521:529	valuable biogenic composite	503:529	valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect	503:634	Here we aimed to valorize the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before.
33262077	5	21	theme	valuable	1554:1561	arg1	source					1464:1469	a natural source	1454:1469	a natural source of phosphate-carbonate minerals	1454:1501	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	5	21	theme	valuable	1554:1561	arg1	material					1563:1570	valuable material	1554:1570	valuable material for regenerative medicine	1554:1596	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	3	22	theme	peripheral	823:832	arg1	segments					834:841	the peripheral segments	819:841	the peripheral segments	819:841	Employing multi-laser Raman spectroscopy and imaging, supported by x-ray diffraction and high-resolution electron microscopy, we discover that the peripheral segments anatomically known as claws and telson, featured completely different composition and morphology, suggesting different applicability.
33262077	6	23	theme	blue	1789:1792	arg1	avenue					1805:1810	blue bioeconomy avenue	1789:1810	blue bioeconomy avenue	1789:1810	Knowledge-based approach on this bio-template is the basis for smart recycling of such fishery waste for sustainable development, by opening channels for blue bioeconomy avenue.
33262077	0	24	theme	biogenic	94:101	arg1	composite					103:111	valuable biogenic composite	85:111	valuable biogenic composite	85:111	Promoting hidden natural design templates in wasted shells of the mantis shrimp into valuable biogenic composite.
33262077	2	25	theme	mantis	431:436	arg1	shrimp					438:443	the astonishing mantis shrimp	415:443	the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before	415:673	Here we aimed to valorize the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before.
33262077	0	26	from	templates	32:40	arg1	shells					52:57	wasted shells	45:57	wasted shells of the mantis shrimp	45:78	Promoting hidden natural design templates in wasted shells of the mantis shrimp into valuable biogenic composite.
33262077	2	27	theme	seafood	483:489	arg1	industry					491:498	seafood industry	483:498	seafood industry	483:498	Here we aimed to valorize the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before.
33262077	0	28	theme	natural	17:23	arg1	templates					32:40	hidden natural design templates	10:40	hidden natural design templates in wasted shells of the mantis shrimp	10:78	Promoting hidden natural design templates in wasted shells of the mantis shrimp into valuable biogenic composite.
33262077	5	29	theme	Resonance	1234:1242	arg1	spectroscopy					1250:1261	Resonance Raman spectroscopy	1234:1261	Resonance Raman spectroscopy	1234:1261	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	4	30	theme	fluorapatite	1037:1048	arg1	coating					1050:1056	fluorapatite coating	1037:1056	fluorapatite coating	1037:1056	The claw presents a bulk Mg-CaCO3 structure reinforced with fluorapatite coating, while the carotenoid-rich telson presents a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold.
33262077	2	31	theme	shell	445:449	arg1	waste					451:455	shell waste	445:455	the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before	415:673	Here we aimed to valorize the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before.
33262077	0	32	theme	wasted	45:50	arg1	shells					52:57	wasted shells	45:57	wasted shells of the mantis shrimp	45:78	Promoting hidden natural design templates in wasted shells of the mantis shrimp into valuable biogenic composite.
33262077	1	33	theme	amazing	291:297	arg1	properties					310:319	its amazing mechanical properties	287:319	its amazing mechanical properties which were studied from evolutionary and ecological points of view	287:386	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	4	34	from	scaffold	1224:1231	arg1	mixture					1152:1158	an amorphous mixture	1139:1158	an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold	1139:1231	The claw presents a bulk Mg-CaCO3 structure reinforced with fluorapatite coating, while the carotenoid-rich telson presents a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold.
33262077	5	35	theme	carotenoid	1304:1313	arg1	pools					1283:1287	concentrated pools	1270:1287	concentrated pools of astaxanthin carotenoid	1270:1313	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	4	36	from	deposition	1191:1200	arg1	structure					1126:1134	a porous and anisotropic structure	1101:1134	a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold	1101:1231	The claw presents a bulk Mg-CaCO3 structure reinforced with fluorapatite coating, while the carotenoid-rich telson presents a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold.
33262077	4	36	from	deposition	1191:1200	arg1	scaffold					1224:1231	the chitin-protein scaffold	1205:1231	the chitin-protein scaffold	1205:1231	The claw presents a bulk Mg-CaCO3 structure reinforced with fluorapatite coating, while the carotenoid-rich telson presents a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold.
33262077	5	37	theme	culture	1616:1622	arg1	substrate					1624:1632	a cell culture substrate	1609:1632	a cell culture substrate	1609:1632	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	3	38	theme	multi-laser	686:696	arg1	spectroscopy					704:715	multi-laser Raman spectroscopy	686:715	multi-laser Raman spectroscopy	686:715	Employing multi-laser Raman spectroscopy and imaging, supported by x-ray diffraction and high-resolution electron microscopy, we discover that the peripheral segments anatomically known as claws and telson, featured completely different composition and morphology, suggesting different applicability.
33262077	5	39	theme	minerals	1494:1501	arg1	source					1464:1469	a natural source	1454:1469	a natural source of phosphate-carbonate minerals	1454:1501	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	5	39	theme	minerals	1494:1501	arg1	biofertilizer					1518:1530	biofertilizer	1518:1530	biofertilizer	1518:1530	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	5	39	theme	minerals	1494:1501	arg1	pollutant					1533:1541	pollutant adsorbent	1533:1551	pollutant adsorbent	1533:1551	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	5	39	theme	minerals	1494:1501	arg1	antioxidants					1504:1515	antioxidants	1504:1515	antioxidants	1504:1515	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	5	39	theme	minerals	1494:1501	arg1	potential					1412:1420	this material's potential	1396:1420	this material's potential for selective applicability	1396:1448	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	5	39	theme	minerals	1494:1501	arg1	material					1563:1570	valuable material	1554:1570	valuable material for regenerative medicine	1554:1596	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	1	40	theme	research	241:248	arg1	attention					250:258	research attention	241:258	research attention from various fields	241:278	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	2	41	theme	novel	578:582	arg1	materials					591:599	novel, smart materials	578:599	novel, smart materials	578:599	Here we aimed to valorize the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before.
33262077	4	42	theme	CaPO4	1173:1177	arg1	mixture					1152:1158	an amorphous mixture	1139:1158	an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold	1139:1231	The claw presents a bulk Mg-CaCO3 structure reinforced with fluorapatite coating, while the carotenoid-rich telson presents a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold.
33262077	1	43	from	fields	273:278	arg1	attention					250:258	research attention	241:258	research attention from various fields	241:278	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	1	44	theme	Squilla	160:166	arg1	Mantis					168:173	the mantis shrimps Squilla Mantis	141:173	the mantis shrimps Squilla Mantis	141:173	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	4	45	theme	gradient	1182:1189	arg1	deposition					1191:1200	gradient deposition	1182:1200	gradient deposition on the chitin-protein scaffold	1182:1231	The claw presents a bulk Mg-CaCO3 structure reinforced with fluorapatite coating, while the carotenoid-rich telson presents a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold.
33262077	5	46	theme	natural	1456:1462	arg1	source					1464:1469	a natural source	1454:1469	a natural source of phosphate-carbonate minerals	1454:1501	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	5	46	theme	natural	1456:1462	arg1	biofertilizer					1518:1530	biofertilizer	1518:1530	biofertilizer	1518:1530	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	5	46	theme	natural	1456:1462	arg1	pollutant					1533:1541	pollutant adsorbent	1533:1551	pollutant adsorbent	1533:1551	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	5	46	theme	natural	1456:1462	arg1	antioxidants					1504:1515	antioxidants	1504:1515	antioxidants	1504:1515	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	5	46	theme	natural	1456:1462	arg1	potential					1412:1420	this material's potential	1396:1420	this material's potential for selective applicability	1396:1448	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	5	46	theme	natural	1456:1462	arg1	material					1563:1570	valuable material	1554:1570	valuable material for regenerative medicine	1554:1596	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	4	47	theme	mixture	1152:1158	arg1	structure					1126:1134	a porous and anisotropic structure	1101:1134	a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold	1101:1231	The claw presents a bulk Mg-CaCO3 structure reinforced with fluorapatite coating, while the carotenoid-rich telson presents a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold.
33262077	6	48	theme	opening	1768:1774	arg1	channels					1776:1783	opening channels	1768:1783	opening channels	1768:1783	Knowledge-based approach on this bio-template is the basis for smart recycling of such fishery waste for sustainable development, by opening channels for blue bioeconomy avenue.
33262077	4	49	theme	CaCO3	1163:1167	arg1	mixture					1152:1158	an amorphous mixture	1139:1158	an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold	1139:1231	The claw presents a bulk Mg-CaCO3 structure reinforced with fluorapatite coating, while the carotenoid-rich telson presents a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold.
33262077	3	50	theme	x-ray	743:747	arg1	diffraction					749:759	x-ray diffraction	743:759	x-ray diffraction	743:759	Employing multi-laser Raman spectroscopy and imaging, supported by x-ray diffraction and high-resolution electron microscopy, we discover that the peripheral segments anatomically known as claws and telson, featured completely different composition and morphology, suggesting different applicability.
33262077	2	51	attach	derived	531:537	arg2	composite					521:529	valuable biogenic composite	503:529	valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect	503:634	Here we aimed to valorize the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before.
33262077	2	51	attach	derived	531:537	arg1	aspect					629:634	aspect	629:634	aspect	629:634	Here we aimed to valorize the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before.
33262077	2	51	attach	derived	531:537	arg1	nature					544:549	nature	544:549	nature	544:549	Here we aimed to valorize the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before.
33262077	2	51	attach	derived	531:537	arg1	by-products					616:626	potentially re-usable for novel, smart materials or added-value by-products	552:626	potentially re-usable for novel, smart materials or added-value by-products	552:626	Here we aimed to valorize the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before.
33262077	4	52	from	mixture	1152:1158	arg1	deposition					1191:1200	gradient deposition	1182:1200	gradient deposition on the chitin-protein scaffold	1182:1231	The claw presents a bulk Mg-CaCO3 structure reinforced with fluorapatite coating, while the carotenoid-rich telson presents a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold.
33262077	4	52	from	mixture	1152:1158	arg1	scaffold					1224:1231	the chitin-protein scaffold	1205:1231	the chitin-protein scaffold	1205:1231	The claw presents a bulk Mg-CaCO3 structure reinforced with fluorapatite coating, while the carotenoid-rich telson presents a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold.
33262077	2	53	dep	novel	578:582	arg1	smart					585:589	smart	585:589	smart	585:589	Here we aimed to valorize the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before.
33262077	2	54	theme	re-usable	564:572	arg1	nature					544:549	nature	544:549	nature	544:549	Here we aimed to valorize the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before.
33262077	2	54	theme	re-usable	564:572	arg1	by-products					616:626	potentially re-usable for novel, smart materials or added-value by-products	552:626	potentially re-usable for novel, smart materials or added-value by-products	552:626	Here we aimed to valorize the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before.
33262077	6	55	theme	sustainable	1740:1750	arg1	development					1752:1762	sustainable development	1740:1762	sustainable development	1740:1762	Knowledge-based approach on this bio-template is the basis for smart recycling of such fishery waste for sustainable development, by opening channels for blue bioeconomy avenue.
33262077	5	56	theme	visible	1343:1349	arg1	spots					1337:1341	the bright red spots	1322:1341	the bright red spots visible on telson	1322:1359	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	3	57	theme	electron	781:788	arg1	microscopy					790:799	high-resolution electron microscopy	765:799	high-resolution electron microscopy	765:799	Employing multi-laser Raman spectroscopy and imaging, supported by x-ray diffraction and high-resolution electron microscopy, we discover that the peripheral segments anatomically known as claws and telson, featured completely different composition and morphology, suggesting different applicability.
33262077	1	58	theme	mineralized	118:128	arg1	armors					218:223	armors	218:223	armors	218:223	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	1	58	theme	mineralized	118:128	arg1	spears					207:212	spears	207:212	spears	207:212	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	1	58	theme	mineralized	118:128	arg1	cuticle					130:136	The mineralized cuticle	114:136	The mineralized cuticle	114:136	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	1	58	theme	mineralized	118:128	arg1	hammers					198:204	natural hammers	190:204	natural hammers	190:204	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	6	59	theme	waste	1730:1734	arg1	recycling					1704:1712	smart recycling	1698:1712	smart recycling of such fishery waste	1698:1734	Knowledge-based approach on this bio-template is the basis for smart recycling of such fishery waste for sustainable development, by opening channels for blue bioeconomy avenue.
33262077	4	60	theme	bulk	997:1000	arg1	structure					1011:1019	a bulk Mg-CaCO3 structure	995:1019	a bulk Mg-CaCO3 structure reinforced with fluorapatite coating	995:1056	The claw presents a bulk Mg-CaCO3 structure reinforced with fluorapatite coating, while the carotenoid-rich telson presents a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold.
33262077	4	61	theme	chitin-protein	1209:1222	arg1	scaffold					1224:1231	the chitin-protein scaffold	1205:1231	the chitin-protein scaffold	1205:1231	The claw presents a bulk Mg-CaCO3 structure reinforced with fluorapatite coating, while the carotenoid-rich telson presents a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold.
33262077	0	62	theme	shrimp	73:78	arg1	shells					52:57	wasted shells	45:57	wasted shells of the mantis shrimp	45:78	Promoting hidden natural design templates in wasted shells of the mantis shrimp into valuable biogenic composite.
33262077	1	63	theme	mantis	145:150	arg1	Mantis					168:173	the mantis shrimps Squilla Mantis	141:173	the mantis shrimps Squilla Mantis	141:173	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	3	64	theme	different	903:911	arg1	composition					913:923	composition	913:923	composition	913:923	Employing multi-laser Raman spectroscopy and imaging, supported by x-ray diffraction and high-resolution electron microscopy, we discover that the peripheral segments anatomically known as claws and telson, featured completely different composition and morphology, suggesting different applicability.
33262077	5	65	theme	red	1333:1335	arg1	spots					1337:1341	the bright red spots	1322:1341	the bright red spots visible on telson	1322:1359	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	0	66	theme	valuable	85:92	arg1	composite					103:111	valuable biogenic composite	85:111	valuable biogenic composite	85:111	Promoting hidden natural design templates in wasted shells of the mantis shrimp into valuable biogenic composite.
33262077	2	67	theme	valuable	503:510	arg1	composite					521:529	valuable biogenic composite	503:529	valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect	503:634	Here we aimed to valorize the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before.
33262077	6	68	theme	bioeconomy	1794:1803	arg1	avenue					1805:1810	blue bioeconomy avenue	1789:1810	blue bioeconomy avenue	1789:1810	Knowledge-based approach on this bio-template is the basis for smart recycling of such fishery waste for sustainable development, by opening channels for blue bioeconomy avenue.
33262077	1	69	theme	evolutionary	345:356	arg1	points					373:378	evolutionary and ecological points	345:378	evolutionary and ecological points of view	345:386	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	6	70	theme	smart	1698:1702	arg1	recycling					1704:1712	smart recycling	1698:1712	smart recycling of such fishery waste	1698:1734	Knowledge-based approach on this bio-template is the basis for smart recycling of such fishery waste for sustainable development, by opening channels for blue bioeconomy avenue.
33262077	0	71	theme	hidden	10:15	arg1	templates					32:40	hidden natural design templates	10:40	hidden natural design templates in wasted shells of the mantis shrimp	10:78	Promoting hidden natural design templates in wasted shells of the mantis shrimp into valuable biogenic composite.
33262077	1	72	theme	ecological	362:371	arg1	points					373:378	evolutionary and ecological points	345:378	evolutionary and ecological points of view	345:386	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	2	73	theme	astonishing	419:429	arg1	shrimp					438:443	the astonishing mantis shrimp	415:443	the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before	415:673	Here we aimed to valorize the astonishing mantis shrimp shell waste resulted from fishery and seafood industry as valuable biogenic composite derived from nature, potentially re-usable for novel, smart materials or added-value by-products, aspect which was not deeply considered before.
33262077	0	74	theme	design	25:30	arg1	templates					32:40	hidden natural design templates	10:40	hidden natural design templates in wasted shells of the mantis shrimp	10:78	Promoting hidden natural design templates in wasted shells of the mantis shrimp into valuable biogenic composite.
33262077	1	75	theme	various	265:271	arg1	fields					273:278	various fields	265:278	various fields	265:278	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	4	76	theme	carotenoid-rich	1069:1083	arg1	telson					1085:1090	the carotenoid-rich telson	1065:1090	the carotenoid-rich telson	1065:1090	The claw presents a bulk Mg-CaCO3 structure reinforced with fluorapatite coating, while the carotenoid-rich telson presents a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold.
33262077	5	77	theme	Raman	1244:1248	arg1	spectroscopy					1250:1261	Resonance Raman spectroscopy	1234:1261	Resonance Raman spectroscopy	1234:1261	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	3	78	theme	different	952:960	arg1	applicability					962:974	different applicability	952:974	different applicability	952:974	Employing multi-laser Raman spectroscopy and imaging, supported by x-ray diffraction and high-resolution electron microscopy, we discover that the peripheral segments anatomically known as claws and telson, featured completely different composition and morphology, suggesting different applicability.
33262077	5	79	theme	cell	1611:1614	arg1	substrate					1624:1632	a cell culture substrate	1609:1632	a cell culture substrate	1609:1632	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	5	80	theme	selective	1426:1434	arg1	applicability					1436:1448	selective applicability	1426:1448	selective applicability	1426:1448	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	5	81	theme	regenerative	1576:1587	arg1	medicine					1589:1596	regenerative medicine	1576:1596	regenerative medicine	1576:1596	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	1	82	theme	mechanical	299:308	arg1	properties					310:319	its amazing mechanical properties	287:319	its amazing mechanical properties which were studied from evolutionary and ecological points of view	287:386	The mineralized cuticle of the mantis shrimps Squilla Mantis which serve as natural hammers, spears and armors, have attracted research attention from various fields due to its amazing mechanical properties which were studied from evolutionary and ecological points of view.
33262077	4	83	theme	anisotropic	1114:1124	arg1	structure					1126:1134	a porous and anisotropic structure	1101:1134	a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold	1101:1231	The claw presents a bulk Mg-CaCO3 structure reinforced with fluorapatite coating, while the carotenoid-rich telson presents a porous and anisotropic structure of an amorphous mixture of CaCO3 and CaPO4 in gradient deposition on the chitin-protein scaffold.
33262077	5	84	theme	astaxanthin	1292:1302	arg1	carotenoid					1304:1313	astaxanthin carotenoid	1292:1313	astaxanthin carotenoid	1292:1313	Resonance Raman spectroscopy showed concentrated pools of astaxanthin carotenoid within the bright red spots visible on telson, Based on our findings, we discuss this material's potential for selective applicability, as a natural source of phosphate-carbonate minerals, antioxidants, biofertilizer, pollutant adsorbent, valuable material for regenerative medicine or even as a cell culture substrate.
33262077	3	85	theme	Raman	698:702	arg1	spectroscopy					704:715	multi-laser Raman spectroscopy	686:715	multi-laser Raman spectroscopy	686:715	Employing multi-laser Raman spectroscopy and imaging, supported by x-ray diffraction and high-resolution electron microscopy, we discover that the peripheral segments anatomically known as claws and telson, featured completely different composition and morphology, suggesting different applicability.
33147550	0	0	theme	human	75:79	arg1	dentine					81:87	human dentine	75:87	human dentine	75:87	Antimicrobial activity and inhibition of biofilm formation in vitro and on human dentine by silver nanoparticles/carboxymethyl-cellulose composites.
33147550	7	1	theme	significant	1169:1179	arg1	reduction					1205:1213	significant (p < 0.05) 58 % biofilm reduction	1169:1213	significant (p < 0.05) 58 % biofilm reduction by 4.8 μg/mL AgNPs/CMC composite	1169:1246	We also demonstrated significant (p < 0.05) 58 % biofilm reduction by 4.8 μg/mL AgNPs/CMC composite on human dentine discs.
33147550	1	2	theme	antimicrobial	175:187	arg1	activity					189:196	the antimicrobial activity	171:196	the antimicrobial activity of a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms	171:320	OBJECTIVE To evaluate the antimicrobial activity of a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms.
33147550	2	3	from	effect	350:355	arg1	viability					405:413	normal human gingival fibroblast cells (HGF) viability	360:413	normal human gingival fibroblast cells (HGF) viability	360:413	DESIGN AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability was determined by the MTT reduction assay.
33147550	0	4	from	activity	14:21	arg1	dentine					81:87	human dentine	75:87	human dentine	75:87	Antimicrobial activity and inhibition of biofilm formation in vitro and on human dentine by silver nanoparticles/carboxymethyl-cellulose composites.
33147550	4	5	theme	22.3 nm	740:746	arg1	particle-size					748:760	a mean 22.3 nm particle-size	733:760	a mean 22.3 nm particle-size	733:760	RESULTS Quasi-spherical AgNPs/CMC composites, with a mean 22.3 nm particle-size were synthesized.
33147550	3	6	dep	Fusobacterium	580:592	arg1	nucleatum					594:602	Fusobacterium nucleatum	580:602	Fusobacterium nucleatum growth	580:609	In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.
33147550	3	7	theme	antimicrobial	488:500	arg1	effect					502:507	the antimicrobial effect	484:507	the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro	484:618	In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.
33147550	3	8	theme	disc	667:670	arg1	biofilms					672:679	dentine disc biofilms	659:679	dentine disc biofilms	659:679	In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.
33147550	1	9	from	activity	189:196	arg1	biofilms					313:320	in vitro and dentine disc heterogeneous biofilms	273:320	in vitro and dentine disc heterogeneous biofilms	273:320	OBJECTIVE To evaluate the antimicrobial activity of a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms.
33147550	2	10	theme	composite	340:348	arg1	effect					350:355	DESIGN AgNPs/CMC composite effect	323:355	DESIGN AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability	323:413	DESIGN AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability was determined by the MTT reduction assay.
33147550	6	11	dep	F.	1037:1038	arg1	nucleatum					1040:1048	F. nucleatum	1037:1048	F. nucleatum	1037:1048	In vitro, they inhibited up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth at concentrations ranging from 1.2 μg/mL to 9.6 μg/mL, as compared with untreated control.
33147550	1	12	theme	heterogeneous	299:311	arg1	biofilms					313:320	in vitro and dentine disc heterogeneous biofilms	273:320	in vitro and dentine disc heterogeneous biofilms	273:320	OBJECTIVE To evaluate the antimicrobial activity of a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms.
33147550	6	13	theme	untreated	1129:1137	arg1	control					1139:1145	untreated control	1129:1145	untreated control	1129:1145	In vitro, they inhibited up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth at concentrations ranging from 1.2 μg/mL to 9.6 μg/mL, as compared with untreated control.
33147550	8	14	theme	AgNPs/CMC	1283:1291	arg1	composite					1293:1301	CONCLUSION AgNPs/CMC composite	1272:1301	CONCLUSION AgNPs/CMC composite	1272:1301	CONCLUSION AgNPs/CMC composite showed anti biofilm activity on monocultures, heterogenous cultures, and dentine discs, resulting a potentially effective alternative to prevent and eliminate infections after endodontic treatment.
33147550	2	15	theme	AgNPs/CMC	330:338	arg1	effect					350:355	DESIGN AgNPs/CMC composite effect	323:355	DESIGN AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability	323:413	DESIGN AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability was determined by the MTT reduction assay.
33147550	3	16	theme	composite	522:530	arg1	biofilms					672:679	dentine disc biofilms	659:679	dentine disc biofilms	659:679	In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.
33147550	3	16	theme	composite	522:530	arg1	effect					502:507	the antimicrobial effect	484:507	the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro	484:618	In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.
33147550	3	16	theme	composite	522:530	arg1	biofilms					638:645	heterogeneous biofilms	624:645	heterogeneous biofilms	624:645	In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.
33147550	0	17	theme	nanoparticles/carboxymethyl-cellulose	99:135	arg1	composites					137:146	silver nanoparticles/carboxymethyl-cellulose composites	92:146	silver nanoparticles/carboxymethyl-cellulose composites	92:146	Antimicrobial activity and inhibition of biofilm formation in vitro and on human dentine by silver nanoparticles/carboxymethyl-cellulose composites.
33147550	7	18	theme	human	1251:1255	arg1	discs					1265:1269	human dentine discs	1251:1269	human dentine discs	1251:1269	We also demonstrated significant (p < 0.05) 58 % biofilm reduction by 4.8 μg/mL AgNPs/CMC composite on human dentine discs.
33147550	2	19	theme	DESIGN	323:328	arg1	effect					350:355	DESIGN AgNPs/CMC composite effect	323:355	DESIGN AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability	323:413	DESIGN AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability was determined by the MTT reduction assay.
33147550	7	20	theme	dentine	1257:1263	arg1	discs					1265:1269	human dentine discs	1251:1269	human dentine discs	1251:1269	We also demonstrated significant (p < 0.05) 58 % biofilm reduction by 4.8 μg/mL AgNPs/CMC composite on human dentine discs.
33147550	2	21	theme	MTT	437:439	arg1	assay					451:455	the MTT reduction assay	433:455	the MTT reduction assay	433:455	DESIGN AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability was determined by the MTT reduction assay.
33147550	0	22	theme	silver	92:97	arg1	composites					137:146	silver nanoparticles/carboxymethyl-cellulose composites	92:146	silver nanoparticles/carboxymethyl-cellulose composites	92:146	Antimicrobial activity and inhibition of biofilm formation in vitro and on human dentine by silver nanoparticles/carboxymethyl-cellulose composites.
33147550	6	23	theme	1.2	1088:1090	arg1	μg/mL					1092:1096	μg/mL	1092:1096	μg/mL	1092:1096	In vitro, they inhibited up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth at concentrations ranging from 1.2 μg/mL to 9.6 μg/mL, as compared with untreated control.
33147550	6	24	theme	%	1005:1005	arg1	growth					1050:1055	up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth	980:1055	up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth	980:1055	In vitro, they inhibited up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth at concentrations ranging from 1.2 μg/mL to 9.6 μg/mL, as compared with untreated control.
33147550	4	25	theme	AgNPs/CMC	706:714	arg1	composites					716:725	Quasi-spherical AgNPs/CMC composites	690:725	Quasi-spherical AgNPs/CMC composites	690:725	RESULTS Quasi-spherical AgNPs/CMC composites, with a mean 22.3 nm particle-size were synthesized.
33147550	7	26	theme	AgNPs/CMC	1228:1236	arg1	composite					1238:1246	4.8 μg/mL AgNPs/CMC composite	1218:1246	4.8 μg/mL AgNPs/CMC composite	1218:1246	We also demonstrated significant (p < 0.05) 58 % biofilm reduction by 4.8 μg/mL AgNPs/CMC composite on human dentine discs.
33147550	2	27	theme	human	367:371	arg1	cells					393:397	normal human gingival fibroblast cells	360:397	normal human gingival fibroblast cells (HGF) viability	360:413	DESIGN AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability was determined by the MTT reduction assay.
33147550	2	27	theme	human	367:371	arg1	HGF					400:402	HGF	400:402	HGF	400:402	DESIGN AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability was determined by the MTT reduction assay.
33147550	1	28	theme	silver	203:208	arg1	composite					260:268	a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite	201:268	a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms	201:320	OBJECTIVE To evaluate the antimicrobial activity of a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms.
33147550	0	29	theme	Antimicrobial	0:12	arg1	activity					14:21	Antimicrobial activity	0:21	Antimicrobial activity	0:21	Antimicrobial activity and inhibition of biofilm formation in vitro and on human dentine by silver nanoparticles/carboxymethyl-cellulose composites.
33147550	6	30	theme	%	989:989	arg1	growth					1050:1055	up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth	980:1055	up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth	980:1055	In vitro, they inhibited up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth at concentrations ranging from 1.2 μg/mL to 9.6 μg/mL, as compared with untreated control.
33147550	6	31	dep	67	986:987	arg1	to					983:984	to	983:984	to	983:984	In vitro, they inhibited up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth at concentrations ranging from 1.2 μg/mL to 9.6 μg/mL, as compared with untreated control.
33147550	2	32	theme	normal	360:365	arg1	cells					393:397	normal human gingival fibroblast cells	360:397	normal human gingival fibroblast cells (HGF) viability	360:413	DESIGN AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability was determined by the MTT reduction assay.
33147550	2	32	theme	normal	360:365	arg1	HGF					400:402	HGF	400:402	HGF	400:402	DESIGN AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability was determined by the MTT reduction assay.
33147550	8	33	theme	anti	1310:1313	arg1	activity					1323:1330	anti biofilm activity	1310:1330	anti biofilm activity	1310:1330	CONCLUSION AgNPs/CMC composite showed anti biofilm activity on monocultures, heterogenous cultures, and dentine discs, resulting a potentially effective alternative to prevent and eliminate infections after endodontic treatment.
33147550	1	34	theme	nanoparticles/carboxymethyl-cellulose	210:246	arg1	composite					260:268	a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite	201:268	a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms	201:320	OBJECTIVE To evaluate the antimicrobial activity of a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms.
33147550	8	35	theme	effective	1415:1423	arg1	alternative					1425:1435	a potentially effective alternative	1401:1435	a potentially effective alternative to prevent and eliminate infections after endodontic treatment	1401:1498	CONCLUSION AgNPs/CMC composite showed anti biofilm activity on monocultures, heterogenous cultures, and dentine discs, resulting a potentially effective alternative to prevent and eliminate infections after endodontic treatment.
33147550	7	36	dep	significant	1169:1179	arg1	p <					1182:1184	p < 0.05	1182:1189	p < 0.05	1182:1189	We also demonstrated significant (p < 0.05) 58 % biofilm reduction by 4.8 μg/mL AgNPs/CMC composite on human dentine discs.
33147550	3	37	from	biofilms	638:645	arg1	albicans					543:550	Candida albicans	535:550	Candida albicans	535:550	In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.
33147550	3	37	from	biofilms	638:645	arg1	growth					604:609	Fusobacterium nucleatum growth	580:609	Fusobacterium nucleatum growth	580:609	In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.
33147550	3	37	from	biofilms	638:645	arg1	faecalis					566:573	Enterococcus faecalis	553:573	Enterococcus faecalis	553:573	In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.
33147550	5	38	theme	significant	883:893	arg1	cytotoxicity					895:906	significant cytotoxicity	883:906	significant cytotoxicity (89 %, p < 0.05)	883:923	They were not toxic to HGF cells at concentrations tested that were antimicrobial, however they caused significant cytotoxicity (89 %, p < 0.05) at concentrations > 15 μg/mL.
33147550	8	39	theme	biofilm	1315:1321	arg1	activity					1323:1330	anti biofilm activity	1310:1330	anti biofilm activity	1310:1330	CONCLUSION AgNPs/CMC composite showed anti biofilm activity on monocultures, heterogenous cultures, and dentine discs, resulting a potentially effective alternative to prevent and eliminate infections after endodontic treatment.
33147550	1	40	from	biofilms	313:320	arg1	activity					189:196	the antimicrobial activity	171:196	the antimicrobial activity of a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms	171:320	OBJECTIVE To evaluate the antimicrobial activity of a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms.
33147550	6	41	theme	9.6	1101:1103	arg1	μg/mL					1092:1096	μg/mL	1092:1096	μg/mL	1092:1096	In vitro, they inhibited up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth at concentrations ranging from 1.2 μg/mL to 9.6 μg/mL, as compared with untreated control.
33147550	1	42	theme	AgNPs/CMC	249:257	arg1	composite					260:268	a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite	201:268	a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms	201:320	OBJECTIVE To evaluate the antimicrobial activity of a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms.
33147550	0	43	theme	biofilm	41:47	arg1	formation					49:57	biofilm formation	41:57	biofilm formation in vitro	41:66	Antimicrobial activity and inhibition of biofilm formation in vitro and on human dentine by silver nanoparticles/carboxymethyl-cellulose composites.
33147550	3	44	theme	Candida	535:541	arg1	albicans					543:550	Candida albicans	535:550	Candida albicans	535:550	In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.
33147550	7	45	theme	%	1195:1195	arg1	reduction					1205:1213	significant (p < 0.05) 58 % biofilm reduction	1169:1213	significant (p < 0.05) 58 % biofilm reduction by 4.8 μg/mL AgNPs/CMC composite	1169:1246	We also demonstrated significant (p < 0.05) 58 % biofilm reduction by 4.8 μg/mL AgNPs/CMC composite on human dentine discs.
33147550	8	46	theme	heterogenous	1349:1360	arg1	cultures					1362:1369	heterogenous cultures	1349:1369	heterogenous cultures	1349:1369	CONCLUSION AgNPs/CMC composite showed anti biofilm activity on monocultures, heterogenous cultures, and dentine discs, resulting a potentially effective alternative to prevent and eliminate infections after endodontic treatment.
33147550	4	47	theme	Quasi-spherical	690:704	arg1	composites					716:725	Quasi-spherical AgNPs/CMC composites	690:725	Quasi-spherical AgNPs/CMC composites	690:725	RESULTS Quasi-spherical AgNPs/CMC composites, with a mean 22.3 nm particle-size were synthesized.
33147550	6	48	theme	%	995:995	arg1	growth					1050:1055	up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth	980:1055	up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth	980:1055	In vitro, they inhibited up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth at concentrations ranging from 1.2 μg/mL to 9.6 μg/mL, as compared with untreated control.
33147550	4	49	with	composites	716:725	arg1	particle-size					748:760	a mean 22.3 nm particle-size	733:760	a mean 22.3 nm particle-size	733:760	RESULTS Quasi-spherical AgNPs/CMC composites, with a mean 22.3 nm particle-size were synthesized.
33147550	4	50	dep	RESULTS	682:688	arg1	composites					716:725	Quasi-spherical AgNPs/CMC composites	690:725	Quasi-spherical AgNPs/CMC composites	690:725	RESULTS Quasi-spherical AgNPs/CMC composites, with a mean 22.3 nm particle-size were synthesized.
33147550	1	51	theme	composite	260:268	arg1	activity					189:196	the antimicrobial activity	171:196	the antimicrobial activity of a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms	171:320	OBJECTIVE To evaluate the antimicrobial activity of a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms.
33147550	3	52	from	biofilms	672:679	arg1	albicans					543:550	Candida albicans	535:550	Candida albicans	535:550	In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.
33147550	3	52	from	biofilms	672:679	arg1	growth					604:609	Fusobacterium nucleatum growth	580:609	Fusobacterium nucleatum growth	580:609	In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.
33147550	3	52	from	biofilms	672:679	arg1	faecalis					566:573	Enterococcus faecalis	553:573	Enterococcus faecalis	553:573	In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.
33147550	6	53	theme	albicans	1010:1017	arg1	growth					1050:1055	up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth	980:1055	up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth	980:1055	In vitro, they inhibited up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth at concentrations ranging from 1.2 μg/mL to 9.6 μg/mL, as compared with untreated control.
33147550	1	54	dep	in	273:274	arg1	vitro					276:280	vitro	276:280	vitro	276:280	OBJECTIVE To evaluate the antimicrobial activity of a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms.
33147550	3	55	theme	heterogeneous	624:636	arg1	biofilms					638:645	heterogeneous biofilms	624:645	heterogeneous biofilms	624:645	In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.
33147550	1	56	from	composite	260:268	arg1	biofilms					313:320	in vitro and dentine disc heterogeneous biofilms	273:320	in vitro and dentine disc heterogeneous biofilms	273:320	OBJECTIVE To evaluate the antimicrobial activity of a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms.
33147550	7	57	theme	4.8	1218:1220	arg1	μg/mL					1222:1226	μg/mL	1222:1226	μg/mL	1222:1226	We also demonstrated significant (p < 0.05) 58 % biofilm reduction by 4.8 μg/mL AgNPs/CMC composite on human dentine discs.
33147550	0	58	theme	formation	49:57	arg1	inhibition					27:36	inhibition	27:36	inhibition of biofilm formation in vitro and on human dentine by silver nanoparticles/carboxymethyl-cellulose composites	27:146	Antimicrobial activity and inhibition of biofilm formation in vitro and on human dentine by silver nanoparticles/carboxymethyl-cellulose composites.
33147550	0	58	theme	formation	49:57	arg1	activity					14:21	Antimicrobial activity	0:21	Antimicrobial activity	0:21	Antimicrobial activity and inhibition of biofilm formation in vitro and on human dentine by silver nanoparticles/carboxymethyl-cellulose composites.
33147550	3	59	theme	AgNPs/CMC	512:520	arg1	composite					522:530	AgNPs/CMC composite	512:530	AgNPs/CMC composite	512:530	In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.
33147550	3	60	from	effect	502:507	arg1	albicans					543:550	Candida albicans	535:550	Candida albicans	535:550	In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.
33147550	3	60	from	effect	502:507	arg1	growth					604:609	Fusobacterium nucleatum growth	580:609	Fusobacterium nucleatum growth	580:609	In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.
33147550	3	60	from	effect	502:507	arg1	faecalis					566:573	Enterococcus faecalis	553:573	Enterococcus faecalis	553:573	In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.
33147550	2	61	theme	cells	393:397	arg1	viability					405:413	normal human gingival fibroblast cells (HGF) viability	360:413	normal human gingival fibroblast cells (HGF) viability	360:413	DESIGN AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability was determined by the MTT reduction assay.
33147550	8	62	theme	dentine	1376:1382	arg1	discs					1384:1388	dentine discs	1376:1388	dentine discs	1376:1388	CONCLUSION AgNPs/CMC composite showed anti biofilm activity on monocultures, heterogenous cultures, and dentine discs, resulting a potentially effective alternative to prevent and eliminate infections after endodontic treatment.
33147550	6	63	dep	μg/mL	1092:1096	arg1	to					1098:1099	to	1098:1099	to	1098:1099	In vitro, they inhibited up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth at concentrations ranging from 1.2 μg/mL to 9.6 μg/mL, as compared with untreated control.
33147550	5	64	from	concentrations	816:829	arg1	toxic					794:798	toxic	794:798	toxic	794:798	They were not toxic to HGF cells at concentrations tested that were antimicrobial, however they caused significant cytotoxicity (89 %, p < 0.05) at concentrations > 15 μg/mL.
33147550	3	65	theme	dentine	659:665	arg1	biofilms					672:679	dentine disc biofilms	659:679	dentine disc biofilms	659:679	In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.
33147550	2	66	theme	fibroblast	382:391	arg1	cells					393:397	normal human gingival fibroblast cells	360:397	normal human gingival fibroblast cells (HGF) viability	360:413	DESIGN AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability was determined by the MTT reduction assay.
33147550	2	66	theme	fibroblast	382:391	arg1	HGF					400:402	HGF	400:402	HGF	400:402	DESIGN AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability was determined by the MTT reduction assay.
33147550	7	67	theme	μg/mL	1222:1226	arg1	composite					1238:1246	4.8 μg/mL AgNPs/CMC composite	1218:1246	4.8 μg/mL AgNPs/CMC composite	1218:1246	We also demonstrated significant (p < 0.05) 58 % biofilm reduction by 4.8 μg/mL AgNPs/CMC composite on human dentine discs.
33147550	3	68	theme	Fusobacterium	580:592	arg1	growth					604:609	Fusobacterium nucleatum growth	580:609	Fusobacterium nucleatum growth	580:609	In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.
33147550	2	69	theme	gingival	373:380	arg1	cells					393:397	normal human gingival fibroblast cells	360:397	normal human gingival fibroblast cells (HGF) viability	360:413	DESIGN AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability was determined by the MTT reduction assay.
33147550	2	69	theme	gingival	373:380	arg1	HGF					400:402	HGF	400:402	HGF	400:402	DESIGN AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability was determined by the MTT reduction assay.
33147550	8	70	theme	CONCLUSION	1272:1281	arg1	composite					1293:1301	CONCLUSION AgNPs/CMC composite	1272:1301	CONCLUSION AgNPs/CMC composite	1272:1301	CONCLUSION AgNPs/CMC composite showed anti biofilm activity on monocultures, heterogenous cultures, and dentine discs, resulting a potentially effective alternative to prevent and eliminate infections after endodontic treatment.
33147550	1	71	theme	in	273:274	arg1	biofilms					313:320	in vitro and dentine disc heterogeneous biofilms	273:320	in vitro and dentine disc heterogeneous biofilms	273:320	OBJECTIVE To evaluate the antimicrobial activity of a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms.
33147550	5	72	theme	HGF	803:805	arg1	cells					807:811	HGF cells	803:811	HGF cells	803:811	They were not toxic to HGF cells at concentrations tested that were antimicrobial, however they caused significant cytotoxicity (89 %, p < 0.05) at concentrations > 15 μg/mL.
33147550	2	73	theme	reduction	441:449	arg1	assay					451:455	the MTT reduction assay	433:455	the MTT reduction assay	433:455	DESIGN AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability was determined by the MTT reduction assay.
33147550	6	74	theme	faecalis	1023:1030	arg1	growth					1050:1055	up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth	980:1055	up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth	980:1055	In vitro, they inhibited up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth at concentrations ranging from 1.2 μg/mL to 9.6 μg/mL, as compared with untreated control.
33147550	4	75	theme	mean	735:738	arg1	particle-size					748:760	a mean 22.3 nm particle-size	733:760	a mean 22.3 nm particle-size	733:760	RESULTS Quasi-spherical AgNPs/CMC composites, with a mean 22.3 nm particle-size were synthesized.
33147550	6	76	theme	F.	1037:1038	arg1	growth					1050:1055	up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth	980:1055	up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth	980:1055	In vitro, they inhibited up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth at concentrations ranging from 1.2 μg/mL to 9.6 μg/mL, as compared with untreated control.
33147550	0	77	from	inhibition	27:36	arg1	dentine					81:87	human dentine	75:87	human dentine	75:87	Antimicrobial activity and inhibition of biofilm formation in vitro and on human dentine by silver nanoparticles/carboxymethyl-cellulose composites.
33147550	5	78	dep	cytotoxicity	895:906	arg1	p <					915:917	p < 0.05	915:922	p < 0.05	915:922	They were not toxic to HGF cells at concentrations tested that were antimicrobial, however they caused significant cytotoxicity (89 %, p < 0.05) at concentrations > 15 μg/mL.
33147550	5	78	dep	cytotoxicity	895:906	arg1	%					912:912	89 %	909:912	89 %	909:912	They were not toxic to HGF cells at concentrations tested that were antimicrobial, however they caused significant cytotoxicity (89 %, p < 0.05) at concentrations > 15 μg/mL.
33147550	7	79	theme	biofilm	1197:1203	arg1	reduction					1205:1213	significant (p < 0.05) 58 % biofilm reduction	1169:1213	significant (p < 0.05) 58 % biofilm reduction by 4.8 μg/mL AgNPs/CMC composite	1169:1246	We also demonstrated significant (p < 0.05) 58 % biofilm reduction by 4.8 μg/mL AgNPs/CMC composite on human dentine discs.
33147550	8	80	theme	endodontic	1479:1488	arg1	treatment					1490:1498	endodontic treatment	1479:1498	endodontic treatment	1479:1498	CONCLUSION AgNPs/CMC composite showed anti biofilm activity on monocultures, heterogenous cultures, and dentine discs, resulting a potentially effective alternative to prevent and eliminate infections after endodontic treatment.
32801287	2	0	from	set	176:178	arg1	producers					228:236	different producers	218:236	different producers	218:236	The quality of a set of commercial heparin injections from different producers was examined by NMR, IR, UV-Vis spectroscopies and potentiometric multisensor system.
32801287	5	1	theme	values	774:779	arg1	semi-estimation					746:760	semi-estimation	746:760	excipients composition as well as semi-estimation of activity values	712:779	UV-Vis and IR, being cheaper than NMR, combined with multivariate statistics were successfully applied to study excipients composition as well as semi-estimation of activity values.
32801287	5	1	theme	values	774:779	arg1	composition					723:733	excipients composition	712:733	excipients composition as well as semi-estimation of activity values	712:779	UV-Vis and IR, being cheaper than NMR, combined with multivariate statistics were successfully applied to study excipients composition as well as semi-estimation of activity values.
32801287	3	2	theme	NMR	494:496	arg1	fingerprints					498:509	1H NMR fingerprints	491:509	1H NMR fingerprints	491:509	The type of raw material regarding heparin animal origin and producer, heparin molecular weight and activity values were derived based on the non-targeted analysis of 1H NMR fingerprints.
32801287	4	3	theme	additives	581:589	arg1	profile					591:597	homogeneity and additives profile	565:597	homogeneity and additives profile	565:597	DOSY NMR spectroscopy was additionally used to study homogeneity and additives profile.
32801287	0	4	theme	Methods	70:76	arg1	Comparison					39:48	Comparison	39:48	Quality Control of Heparin Injections: Comparison of Four Established Methods.	0:77	Quality Control of Heparin Injections: Comparison of Four Established Methods.
32801287	4	5	theme	DOSY	512:515	arg1	spectroscopy					521:532	DOSY NMR spectroscopy	512:532	DOSY NMR spectroscopy	512:532	DOSY NMR spectroscopy was additionally used to study homogeneity and additives profile.
32801287	6	6	theme	additional	852:861	arg1	source					863:868	an important additional source	839:868	an important additional source of information about inorganic composition of finished heparin formulations	839:944	Potentiometric multisensor measurements were found to be an important additional source of information about inorganic composition of finished heparin formulations.
32801287	6	6	theme	additional	852:861	arg1	measurements					809:820	Potentiometric multisensor measurements	782:820	Potentiometric multisensor measurements	782:820	Potentiometric multisensor measurements were found to be an important additional source of information about inorganic composition of finished heparin formulations.
32801287	3	7	theme	fingerprints	498:509	arg1	analysis					479:486	the non-targeted analysis	462:486	the non-targeted analysis of 1H NMR fingerprints	462:509	The type of raw material regarding heparin animal origin and producer, heparin molecular weight and activity values were derived based on the non-targeted analysis of 1H NMR fingerprints.
32801287	4	8	used	used	551:554	arg2	spectroscopy					521:532	DOSY NMR spectroscopy	512:532	DOSY NMR spectroscopy	512:532	DOSY NMR spectroscopy was additionally used to study homogeneity and additives profile.
32801287	3	9	theme	heparin	359:365	arg1	origin					374:379	heparin animal origin	359:379	heparin animal origin	359:379	The type of raw material regarding heparin animal origin and producer, heparin molecular weight and activity values were derived based on the non-targeted analysis of 1H NMR fingerprints.
32801287	6	10	theme	formulations	933:944	arg1	composition					901:911	inorganic composition	891:911	inorganic composition of finished heparin formulations	891:944	Potentiometric multisensor measurements were found to be an important additional source of information about inorganic composition of finished heparin formulations.
32801287	5	11	theme	multivariate	653:664	arg1	statistics					666:675	multivariate statistics	653:675	multivariate statistics	653:675	UV-Vis and IR, being cheaper than NMR, combined with multivariate statistics were successfully applied to study excipients composition as well as semi-estimation of activity values.
32801287	7	12	theme	finished	1003:1010	arg1	injections					1020:1029	finished heparin injections	1003:1029	finished heparin injections	1003:1029	All investigated instrumental techniques are useful for finished heparin injections and should be selected according to availability as well as the information and confidence required for a specific sample.
32801287	2	13	theme	UV-Vis	263:268	arg1	spectroscopies					270:283	UV-Vis spectroscopies	263:283	UV-Vis spectroscopies	263:283	The quality of a set of commercial heparin injections from different producers was examined by NMR, IR, UV-Vis spectroscopies and potentiometric multisensor system.
32801287	3	14	theme	raw	336:338	arg1	material					340:347	raw material	336:347	raw material	336:347	The type of raw material regarding heparin animal origin and producer, heparin molecular weight and activity values were derived based on the non-targeted analysis of 1H NMR fingerprints.
32801287	6	15	theme	heparin	925:931	arg1	formulations					933:944	finished heparin formulations	916:944	finished heparin formulations	916:944	Potentiometric multisensor measurements were found to be an important additional source of information about inorganic composition of finished heparin formulations.
32801287	7	16	theme	heparin	1012:1018	arg1	injections					1020:1029	finished heparin injections	1003:1029	finished heparin injections	1003:1029	All investigated instrumental techniques are useful for finished heparin injections and should be selected according to availability as well as the information and confidence required for a specific sample.
32801287	3	17	theme	material	340:347	arg1	type					328:331	The type	324:331	The type of raw material regarding heparin animal origin and producer, heparin molecular weight and activity values	324:438	The type of raw material regarding heparin animal origin and producer, heparin molecular weight and activity values were derived based on the non-targeted analysis of 1H NMR fingerprints.
32801287	6	18	theme	finished	916:923	arg1	formulations					933:944	finished heparin formulations	916:944	finished heparin formulations	916:944	Potentiometric multisensor measurements were found to be an important additional source of information about inorganic composition of finished heparin formulations.
32801287	3	19	theme	producer	385:392	arg1	values					433:438	producer, heparin molecular weight and activity values	385:438	producer, heparin molecular weight and activity values	385:438	The type of raw material regarding heparin animal origin and producer, heparin molecular weight and activity values were derived based on the non-targeted analysis of 1H NMR fingerprints.
32801287	7	20	dep	information	1095:1105	arg1	the					1091:1093	the	1091:1093	the	1091:1093	All investigated instrumental techniques are useful for finished heparin injections and should be selected according to availability as well as the information and confidence required for a specific sample.
32801287	7	21	theme	specific	1137:1144	arg1	sample					1146:1151	a specific sample	1135:1151	a specific sample	1135:1151	All investigated instrumental techniques are useful for finished heparin injections and should be selected according to availability as well as the information and confidence required for a specific sample.
32801287	2	22	from	producers	228:236	arg1	set					176:178	a set	174:178	a set of commercial heparin injections from different producers	174:236	The quality of a set of commercial heparin injections from different producers was examined by NMR, IR, UV-Vis spectroscopies and potentiometric multisensor system.
32801287	2	22	from	producers	228:236	arg1	injections					202:211	commercial heparin injections	183:211	commercial heparin injections from different producers	183:236	The quality of a set of commercial heparin injections from different producers was examined by NMR, IR, UV-Vis spectroscopies and potentiometric multisensor system.
32801287	0	23	theme	Quality	0:6	arg1	Control					8:14	Quality Control	0:14	Quality Control of Heparin Injections: Comparison of Four Established Methods.	0:77	Quality Control of Heparin Injections: Comparison of Four Established Methods.
32801287	6	24	theme	multisensor	797:807	arg1	measurements					809:820	Potentiometric multisensor measurements	782:820	Potentiometric multisensor measurements	782:820	Potentiometric multisensor measurements were found to be an important additional source of information about inorganic composition of finished heparin formulations.
32801287	6	24	theme	multisensor	797:807	arg1	source					863:868	an important additional source	839:868	an important additional source of information about inorganic composition of finished heparin formulations	839:944	Potentiometric multisensor measurements were found to be an important additional source of information about inorganic composition of finished heparin formulations.
32801287	2	25	theme	multisensor	304:314	arg1	system					316:321	potentiometric multisensor system	289:321	potentiometric multisensor system	289:321	The quality of a set of commercial heparin injections from different producers was examined by NMR, IR, UV-Vis spectroscopies and potentiometric multisensor system.
32801287	3	26	theme	activity	424:431	arg1	values					433:438	producer, heparin molecular weight and activity values	385:438	producer, heparin molecular weight and activity values	385:438	The type of raw material regarding heparin animal origin and producer, heparin molecular weight and activity values were derived based on the non-targeted analysis of 1H NMR fingerprints.
32801287	0	27	theme	Heparin	19:25	arg1	Injections					27:36	Heparin Injections	19:36	Heparin Injections	19:36	Quality Control of Heparin Injections: Comparison of Four Established Methods.
32801287	6	28	theme	Potentiometric	782:795	arg1	measurements					809:820	Potentiometric multisensor measurements	782:820	Potentiometric multisensor measurements	782:820	Potentiometric multisensor measurements were found to be an important additional source of information about inorganic composition of finished heparin formulations.
32801287	6	28	theme	Potentiometric	782:795	arg1	source					863:868	an important additional source	839:868	an important additional source of information about inorganic composition of finished heparin formulations	839:944	Potentiometric multisensor measurements were found to be an important additional source of information about inorganic composition of finished heparin formulations.
32801287	2	29	theme	potentiometric	289:302	arg1	system					316:321	potentiometric multisensor system	289:321	potentiometric multisensor system	289:321	The quality of a set of commercial heparin injections from different producers was examined by NMR, IR, UV-Vis spectroscopies and potentiometric multisensor system.
32801287	7	30	theme	investigated	951:962	arg1	techniques					977:986	All investigated instrumental techniques	947:986	All investigated instrumental techniques	947:986	All investigated instrumental techniques are useful for finished heparin injections and should be selected according to availability as well as the information and confidence required for a specific sample.
32801287	4	31	theme	homogeneity	565:575	arg1	profile					591:597	homogeneity and additives profile	565:597	homogeneity and additives profile	565:597	DOSY NMR spectroscopy was additionally used to study homogeneity and additives profile.
32801287	2	32	theme	set	176:178	arg1	quality					163:169	The quality	159:169	The quality of a set of commercial heparin injections from different producers	159:236	The quality of a set of commercial heparin injections from different producers was examined by NMR, IR, UV-Vis spectroscopies and potentiometric multisensor system.
32801287	1	33	theme	anticoagulant	93:105	arg1	medication					107:116	an anticoagulant medication	90:116	an anticoagulant medication that is usually injected subcutaneously	90:156	Heparin is an anticoagulant medication that is usually injected subcutaneously.
32801287	1	33	theme	anticoagulant	93:105	arg1	Heparin					79:85	Heparin	79:85	Heparin	79:85	Heparin is an anticoagulant medication that is usually injected subcutaneously.
32801287	3	34	theme	molecular	403:411	arg1	weight					413:418	heparin molecular weight	395:418	heparin molecular weight	395:418	The type of raw material regarding heparin animal origin and producer, heparin molecular weight and activity values were derived based on the non-targeted analysis of 1H NMR fingerprints.
32801287	5	35	theme	excipients	712:721	arg1	composition					723:733	excipients composition	712:733	excipients composition as well as semi-estimation of activity values	712:779	UV-Vis and IR, being cheaper than NMR, combined with multivariate statistics were successfully applied to study excipients composition as well as semi-estimation of activity values.
32801287	6	36	theme	inorganic	891:899	arg1	composition					901:911	inorganic composition	891:911	inorganic composition of finished heparin formulations	891:944	Potentiometric multisensor measurements were found to be an important additional source of information about inorganic composition of finished heparin formulations.
32801287	0	37	theme	Injections	27:36	arg1	Control					8:14	Quality Control	0:14	Quality Control of Heparin Injections: Comparison of Four Established Methods.	0:77	Quality Control of Heparin Injections: Comparison of Four Established Methods.
32801287	3	38	theme	weight	413:418	arg1	values					433:438	producer, heparin molecular weight and activity values	385:438	producer, heparin molecular weight and activity values	385:438	The type of raw material regarding heparin animal origin and producer, heparin molecular weight and activity values were derived based on the non-targeted analysis of 1H NMR fingerprints.
32801287	3	39	theme	animal	367:372	arg1	origin					374:379	heparin animal origin	359:379	heparin animal origin	359:379	The type of raw material regarding heparin animal origin and producer, heparin molecular weight and activity values were derived based on the non-targeted analysis of 1H NMR fingerprints.
32801287	4	40	theme	NMR	517:519	arg1	spectroscopy					521:532	DOSY NMR spectroscopy	512:532	DOSY NMR spectroscopy	512:532	DOSY NMR spectroscopy was additionally used to study homogeneity and additives profile.
32801287	6	41	theme	information	873:883	arg1	source					863:868	an important additional source	839:868	an important additional source of information about inorganic composition of finished heparin formulations	839:944	Potentiometric multisensor measurements were found to be an important additional source of information about inorganic composition of finished heparin formulations.
32801287	6	41	theme	information	873:883	arg1	measurements					809:820	Potentiometric multisensor measurements	782:820	Potentiometric multisensor measurements	782:820	Potentiometric multisensor measurements were found to be an important additional source of information about inorganic composition of finished heparin formulations.
32801287	2	42	theme	different	218:226	arg1	producers					228:236	different producers	218:236	different producers	218:236	The quality of a set of commercial heparin injections from different producers was examined by NMR, IR, UV-Vis spectroscopies and potentiometric multisensor system.
32801287	3	43	theme	heparin	395:401	arg1	weight					413:418	heparin molecular weight	395:418	heparin molecular weight	395:418	The type of raw material regarding heparin animal origin and producer, heparin molecular weight and activity values were derived based on the non-targeted analysis of 1H NMR fingerprints.
32801287	2	44	theme	injections	202:211	arg1	set					176:178	a set	174:178	a set of commercial heparin injections from different producers	174:236	The quality of a set of commercial heparin injections from different producers was examined by NMR, IR, UV-Vis spectroscopies and potentiometric multisensor system.
32801287	2	44	theme	injections	202:211	arg1	injections					202:211	commercial heparin injections	183:211	commercial heparin injections from different producers	183:236	The quality of a set of commercial heparin injections from different producers was examined by NMR, IR, UV-Vis spectroscopies and potentiometric multisensor system.
32801287	3	45	theme	1H	491:492	arg1	NMR					494:496	1H NMR	491:496	1H NMR fingerprints	491:509	The type of raw material regarding heparin animal origin and producer, heparin molecular weight and activity values were derived based on the non-targeted analysis of 1H NMR fingerprints.
32801287	5	46	dep	IR	611:612	arg1	cheaper					621:627	cheaper	621:627	cheaper	621:627	UV-Vis and IR, being cheaper than NMR, combined with multivariate statistics were successfully applied to study excipients composition as well as semi-estimation of activity values.
32801287	2	47	theme	heparin	194:200	arg1	injections					202:211	commercial heparin injections	183:211	commercial heparin injections from different producers	183:236	The quality of a set of commercial heparin injections from different producers was examined by NMR, IR, UV-Vis spectroscopies and potentiometric multisensor system.
32801287	3	48	theme	non-targeted	466:477	arg1	analysis					479:486	the non-targeted analysis	462:486	the non-targeted analysis of 1H NMR fingerprints	462:509	The type of raw material regarding heparin animal origin and producer, heparin molecular weight and activity values were derived based on the non-targeted analysis of 1H NMR fingerprints.
32801287	7	49	theme	instrumental	964:975	arg1	techniques					977:986	All investigated instrumental techniques	947:986	All investigated instrumental techniques	947:986	All investigated instrumental techniques are useful for finished heparin injections and should be selected according to availability as well as the information and confidence required for a specific sample.
32801287	2	50	theme	commercial	183:192	arg1	injections					202:211	commercial heparin injections	183:211	commercial heparin injections from different producers	183:236	The quality of a set of commercial heparin injections from different producers was examined by NMR, IR, UV-Vis spectroscopies and potentiometric multisensor system.
32801287	0	51	theme	Established	58:68	arg1	Methods					70:76	Four Established Methods	53:76	Four Established Methods	53:76	Quality Control of Heparin Injections: Comparison of Four Established Methods.
32801287	6	52	theme	important	842:850	arg1	source					863:868	an important additional source	839:868	an important additional source of information about inorganic composition of finished heparin formulations	839:944	Potentiometric multisensor measurements were found to be an important additional source of information about inorganic composition of finished heparin formulations.
32801287	6	52	theme	important	842:850	arg1	measurements					809:820	Potentiometric multisensor measurements	782:820	Potentiometric multisensor measurements	782:820	Potentiometric multisensor measurements were found to be an important additional source of information about inorganic composition of finished heparin formulations.
32801287	0	53	dep	Control	8:14	arg1	Comparison					39:48	Comparison	39:48	Quality Control of Heparin Injections: Comparison of Four Established Methods.	0:77	Quality Control of Heparin Injections: Comparison of Four Established Methods.
32801287	5	54	theme	activity	765:772	arg1	values					774:779	activity values	765:779	activity values	765:779	UV-Vis and IR, being cheaper than NMR, combined with multivariate statistics were successfully applied to study excipients composition as well as semi-estimation of activity values.
32077484	6	0	theme	non-starch	855:864	arg1	polysaccharides					866:880	non-starch polysaccharides	855:880	non-starch polysaccharides (NSP)	855:886	Dextrin, oligosaccharides with 2-6 polymerization degree and non-starch polysaccharides (NSP) make up 15.90-34.83%, 17.59-38.63%, and 2.33-7.47% of the total carbohydrates in beer, respectively.
32077484	6	0	theme	non-starch	855:864	arg1	NSP					883:885	NSP	883:885	NSP	883:885	Dextrin, oligosaccharides with 2-6 polymerization degree and non-starch polysaccharides (NSP) make up 15.90-34.83%, 17.59-38.63%, and 2.33-7.47% of the total carbohydrates in beer, respectively.
32077484	5	1	attach	present	768:774	arg1	amounts					785:791	small amounts	779:791	small amounts	779:791	Monosaccharides were only present in small amounts.
32077484	5	1	attach	present	768:774	arg2	Monosaccharides					742:756	Monosaccharides	742:756	Monosaccharides	742:756	Monosaccharides were only present in small amounts.
32077484	10	2	theme	yeast	1480:1484	arg1	utilization					1486:1496	yeast utilization	1480:1496	yeast utilization	1480:1496	CONCLUSION The oligosaccharides in beer reflect yeast utilization, depending on the type of beer.
32077484	3	3	with	beer	439:442	arg1	adjuncts					449:456	adjuncts	449:456	adjuncts	449:456	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	6	4	from	beer	969:972	arg1	carbohydrates					952:964	the total carbohydrates	942:964	the total carbohydrates in beer	942:972	Dextrin, oligosaccharides with 2-6 polymerization degree and non-starch polysaccharides (NSP) make up 15.90-34.83%, 17.59-38.63%, and 2.33-7.47% of the total carbohydrates in beer, respectively.
32077484	6	4	from	beer	969:972	arg1	%					937:937	2.33-7.47%	928:937	2.33-7.47% of the total carbohydrates in beer	928:972	Dextrin, oligosaccharides with 2-6 polymerization degree and non-starch polysaccharides (NSP) make up 15.90-34.83%, 17.59-38.63%, and 2.33-7.47% of the total carbohydrates in beer, respectively.
32077484	6	4	from	beer	969:972	arg1	%					907:907	15.90-34.83%	896:907	15.90-34.83%	896:907	Dextrin, oligosaccharides with 2-6 polymerization degree and non-starch polysaccharides (NSP) make up 15.90-34.83%, 17.59-38.63%, and 2.33-7.47% of the total carbohydrates in beer, respectively.
32077484	6	4	from	beer	969:972	arg1	%					921:921	17.59-38.63%	910:921	17.59-38.63%	910:921	Dextrin, oligosaccharides with 2-6 polymerization degree and non-starch polysaccharides (NSP) make up 15.90-34.83%, 17.59-38.63%, and 2.33-7.47% of the total carbohydrates in beer, respectively.
32077484	8	5	dep	significantly	1276:1288	arg1	P < 0.05					1291:1298	P < 0.05	1291:1298	P < 0.05	1291:1298	Non-starch polysaccharide, dextrin, trisaccharide, and tetrasaccharide content significantly (P < 0.05) correlated with beer viscosity.
32077484	6	6	theme	2-6	825:827	arg1	polymerization					829:842	polymerization	829:842	polymerization	829:842	Dextrin, oligosaccharides with 2-6 polymerization degree and non-starch polysaccharides (NSP) make up 15.90-34.83%, 17.59-38.63%, and 2.33-7.47% of the total carbohydrates in beer, respectively.
32077484	6	7	from	%	921:921	arg1	beer					969:972	beer	969:972	beer	969:972	Dextrin, oligosaccharides with 2-6 polymerization degree and non-starch polysaccharides (NSP) make up 15.90-34.83%, 17.59-38.63%, and 2.33-7.47% of the total carbohydrates in beer, respectively.
32077484	7	8	theme	NSP	1013:1015	arg1	content					1017:1023	NSP content	1013:1023	NSP content	1013:1023	The dextrin content and NSP content were significantly (P < 0.05) different in wheat beer and barley malt beer, and their content was significantly (P < 0.01) correlated with the content of extracts in beer.
32077484	5	9	theme	small	779:783	arg1	amounts					785:791	small amounts	779:791	small amounts	779:791	Monosaccharides were only present in small amounts.
32077484	5	10	from	amounts	785:791	arg1	present					768:774	present	768:774	present	768:774	Monosaccharides were only present in small amounts.
32077484	0	11	from	influence	57:65	arg1	beers					41:45	commercial beers	30:45	commercial beers	30:45	Profiling of carbohydrates in commercial beers and their influence on beer quality.
32077484	0	11	from	influence	57:65	arg1	quality					75:81	beer quality	70:81	beer quality	70:81	Profiling of carbohydrates in commercial beers and their influence on beer quality.
32077484	4	12	theme	beer	660:663	arg1	types					651:655	three types	645:655	three types of beer, raffinose	645:674	RESULTS Among the oligosaccharides in three types of beer, raffinose was the most, followed by maltotetraose, maltotriose and maltose.
32077484	10	13	theme	beer	1524:1527	arg1	type					1516:1519	the type	1512:1519	the type of beer	1512:1527	CONCLUSION The oligosaccharides in beer reflect yeast utilization, depending on the type of beer.
32077484	3	14	with	beer	405:408	arg1	adjuncts					449:456	adjuncts	449:456	adjuncts	449:456	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	9	15	theme	component	1412:1420	arg1	analysis					1422:1429	principal component analysis	1402:1429	principal component analysis	1402:1429	These beer samples could be categorized clearly into three groups by principal component analysis.
32077484	6	16	theme	total	946:950	arg1	carbohydrates					952:964	the total carbohydrates	942:964	the total carbohydrates in beer	942:972	Dextrin, oligosaccharides with 2-6 polymerization degree and non-starch polysaccharides (NSP) make up 15.90-34.83%, 17.59-38.63%, and 2.33-7.47% of the total carbohydrates in beer, respectively.
32077484	8	17	theme	polysaccharide	1208:1221	arg1	trisaccharide					1233:1245	Non-starch polysaccharide, dextrin, trisaccharide, and tetrasaccharide content	1197:1274	trisaccharide	1233:1245	Non-starch polysaccharide, dextrin, trisaccharide, and tetrasaccharide content significantly (P < 0.05) correlated with beer viscosity.
32077484	3	18	theme	production	559:568	arg1	conditions					570:579	production conditions	559:579	production conditions	559:579	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	0	19	from	carbohydrates	13:25	arg1	beers					41:45	commercial beers	30:45	commercial beers	30:45	Profiling of carbohydrates in commercial beers and their influence on beer quality.
32077484	13	20	theme	Chemical	1798:1805	arg1	Industry					1807:1814	Chemical Industry	1798:1814	Chemical Industry	1798:1814	© 2020 Society of Chemical Industry.
32077484	0	21	theme	beer	70:73	arg1	quality					75:81	beer quality	70:81	beer quality	70:81	Profiling of carbohydrates in commercial beers and their influence on beer quality.
32077484	0	22	from	Profiling	0:8	arg1	beers					41:45	commercial beers	30:45	commercial beers	30:45	Profiling of carbohydrates in commercial beers and their influence on beer quality.
32077484	0	22	from	Profiling	0:8	arg1	quality					75:81	beer quality	70:81	beer quality	70:81	Profiling of carbohydrates in commercial beers and their influence on beer quality.
32077484	3	23	dep	types	366:370	arg1	types					366:370	three types	360:370	three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts -	360:458	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	3	23	dep	types	366:370	arg1	beer					439:442	barley malt beer	427:442	barley malt beer	427:442	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	3	23	dep	types	366:370	arg1	beer					417:420	wheat beer	411:420	wheat beer	411:420	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	3	23	dep	types	366:370	arg1	beer					405:408	barley malt beer	393:408	barley malt beer	393:408	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	5	24	located	present	768:774	arg1	amounts					785:791	small amounts	779:791	small amounts	779:791	Monosaccharides were only present in small amounts.
32077484	5	24	located	present	768:774	arg2	Monosaccharides					742:756	Monosaccharides	742:756	Monosaccharides	742:756	Monosaccharides were only present in small amounts.
32077484	11	25	from	carbohydrates	1601:1613	arg1	beer					1618:1621	beer	1618:1621	beer	1618:1621	Dextrin, oligosaccharides with 2-4 polymerization, and NSP, were major carbohydrates in beer.
32077484	6	26	with	Dextrin	794:800	arg1	degree					844:849	2-6 polymerization degree	825:849	2-6 polymerization degree	825:849	Dextrin, oligosaccharides with 2-6 polymerization degree and non-starch polysaccharides (NSP) make up 15.90-34.83%, 17.59-38.63%, and 2.33-7.47% of the total carbohydrates in beer, respectively.
32077484	6	27	from	%	937:937	arg1	beer					969:972	beer	969:972	beer	969:972	Dextrin, oligosaccharides with 2-6 polymerization degree and non-starch polysaccharides (NSP) make up 15.90-34.83%, 17.59-38.63%, and 2.33-7.47% of the total carbohydrates in beer, respectively.
32077484	3	28	theme	malt	400:403	arg1	types					366:370	three types	360:370	three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts -	360:458	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	3	28	theme	malt	400:403	arg1	beer					405:408	barley malt beer	393:408	barley malt beer	393:408	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	7	29	theme	malt	1090:1093	arg1	beer					1095:1098	barley malt beer	1083:1098	barley malt beer	1083:1098	The dextrin content and NSP content were significantly (P < 0.05) different in wheat beer and barley malt beer, and their content was significantly (P < 0.01) correlated with the content of extracts in beer.
32077484	6	30	with	oligosaccharides	803:818	arg1	degree					844:849	2-6 polymerization degree	825:849	2-6 polymerization degree	825:849	Dextrin, oligosaccharides with 2-6 polymerization degree and non-starch polysaccharides (NSP) make up 15.90-34.83%, 17.59-38.63%, and 2.33-7.47% of the total carbohydrates in beer, respectively.
32077484	0	31	theme	carbohydrates	13:25	arg1	Profiling					0:8	Profiling	0:8	Profiling of carbohydrates in commercial beers	0:45	Profiling of carbohydrates in commercial beers and their influence on beer quality.
32077484	0	31	theme	carbohydrates	13:25	arg1	influence					57:65	their influence	51:65	their influence on beer quality	51:81	Profiling of carbohydrates in commercial beers and their influence on beer quality.
32077484	7	32	dep	significantly	1123:1135	arg1	P					1138:1138	P < 0.01	1138:1145	P < 0.01	1138:1145	The dextrin content and NSP content were significantly (P < 0.05) different in wheat beer and barley malt beer, and their content was significantly (P < 0.01) correlated with the content of extracts in beer.
32077484	7	33	theme	wheat	1068:1072	arg1	beer					1074:1077	wheat beer	1068:1077	wheat beer	1068:1077	The dextrin content and NSP content were significantly (P < 0.05) different in wheat beer and barley malt beer, and their content was significantly (P < 0.01) correlated with the content of extracts in beer.
32077484	0	34	theme	commercial	30:39	arg1	beers					41:45	commercial beers	30:45	commercial beers	30:45	Profiling of carbohydrates in commercial beers and their influence on beer quality.
32077484	3	35	theme	commercial	375:384	arg1	beer					386:389	commercial beer	375:389	commercial beer	375:389	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	11	36	theme	major	1595:1599	arg1	carbohydrates					1601:1613	major carbohydrates	1595:1613	major carbohydrates	1595:1613	Dextrin, oligosaccharides with 2-4 polymerization, and NSP, were major carbohydrates in beer.
32077484	3	37	theme	malt	434:437	arg1	types					366:370	three types	360:370	three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts -	360:458	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	3	37	theme	malt	434:437	arg1	beer					439:442	barley malt beer	427:442	barley malt beer	427:442	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	1	38	from	carbohydrates	99:111	arg1	beer					116:119	beer	116:119	beer	116:119	BACKGROUND The carbohydrates in beer play an important role as they are essential for fermentation.
32077484	12	39	theme	beer	1768:1771	arg1	types					1773:1777	different beer types	1758:1777	different beer types	1758:1777	Their composition and concentration influenced its characteristics and quality, and played an important role in the discrimination of different beer types.
32077484	12	40	theme	important	1718:1726	arg1	role					1728:1731	an important role	1715:1731	an important role	1715:1731	Their composition and concentration influenced its characteristics and quality, and played an important role in the discrimination of different beer types.
32077484	7	41	from	different	1055:1063	arg1	beer					1095:1098	barley malt beer	1083:1098	barley malt beer	1083:1098	The dextrin content and NSP content were significantly (P < 0.05) different in wheat beer and barley malt beer, and their content was significantly (P < 0.01) correlated with the content of extracts in beer.
32077484	7	41	from	different	1055:1063	arg1	beer					1074:1077	wheat beer	1068:1077	wheat beer	1068:1077	The dextrin content and NSP content were significantly (P < 0.05) different in wheat beer and barley malt beer, and their content was significantly (P < 0.01) correlated with the content of extracts in beer.
32077484	3	42	theme	beer	492:495	arg1	quality					497:503	beer quality	492:503	beer quality	492:503	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	4	43	from	oligosaccharides	625:640	arg1	types					651:655	three types	645:655	three types of beer, raffinose	645:674	RESULTS Among the oligosaccharides in three types of beer, raffinose was the most, followed by maltotetraose, maltotriose and maltose.
32077484	2	44	theme	beer	265:268	arg1	characteristics					242:256	the sensory characteristics	230:256	the sensory characteristics of the beer and so their determination is of great interest	230:316	Any change in their composition may influence the sensory characteristics of the beer and so their determination is of great interest.
32077484	9	45	theme	beer	1339:1342	arg1	samples					1344:1350	These beer samples	1333:1350	These beer samples	1333:1350	These beer samples could be categorized clearly into three groups by principal component analysis.
32077484	2	46	from	change	188:193	arg1	composition					204:214	their composition	198:214	their composition	198:214	Any change in their composition may influence the sensory characteristics of the beer and so their determination is of great interest.
32077484	6	47	theme	polymerization	829:842	arg1	degree					844:849	2-6 polymerization degree	825:849	2-6 polymerization degree	825:849	Dextrin, oligosaccharides with 2-6 polymerization degree and non-starch polysaccharides (NSP) make up 15.90-34.83%, 17.59-38.63%, and 2.33-7.47% of the total carbohydrates in beer, respectively.
32077484	7	48	dep	significantly	1030:1042	arg1	P					1045:1045	P < 0.05	1045:1052	P < 0.05	1045:1052	The dextrin content and NSP content were significantly (P < 0.05) different in wheat beer and barley malt beer, and their content was significantly (P < 0.01) correlated with the content of extracts in beer.
32077484	10	49	dep	CONCLUSION	1432:1441	arg1	reflect					1472:1478	reflect	1472:1478	reflect	1472:1478	CONCLUSION The oligosaccharides in beer reflect yeast utilization, depending on the type of beer.
32077484	7	50	from	content	1168:1174	arg1	beer					1191:1194	beer	1191:1194	beer	1191:1194	The dextrin content and NSP content were significantly (P < 0.05) different in wheat beer and barley malt beer, and their content was significantly (P < 0.01) correlated with the content of extracts in beer.
32077484	3	51	with	beer	417:420	arg1	adjuncts					449:456	adjuncts	449:456	adjuncts	449:456	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	7	52	from	beer	1095:1098	arg1	different					1055:1063	different	1055:1063	different	1055:1063	The dextrin content and NSP content were significantly (P < 0.05) different in wheat beer and barley malt beer, and their content was significantly (P < 0.01) correlated with the content of extracts in beer.
32077484	7	52	from	beer	1095:1098	arg1	content					1001:1007	The dextrin content	989:1007	The dextrin content	989:1007	The dextrin content and NSP content were significantly (P < 0.05) different in wheat beer and barley malt beer, and their content was significantly (P < 0.01) correlated with the content of extracts in beer.
32077484	3	53	theme	raw	539:541	arg1	ingredients					543:553	raw ingredients	539:553	raw ingredients	539:553	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	8	54	dep	trisaccharide	1233:1245	arg1	dextrin					1224:1230	Non-starch polysaccharide, dextrin, trisaccharide, and tetrasaccharide content	1197:1274	dextrin	1224:1230	Non-starch polysaccharide, dextrin, trisaccharide, and tetrasaccharide content significantly (P < 0.05) correlated with beer viscosity.
32077484	3	55	from	carbohydrates	343:355	arg1	types					366:370	three types	360:370	three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts -	360:458	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	3	55	from	carbohydrates	343:355	arg1	beer					439:442	barley malt beer	427:442	barley malt beer	427:442	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	3	55	from	carbohydrates	343:355	arg1	beer					417:420	wheat beer	411:420	wheat beer	411:420	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	3	55	from	carbohydrates	343:355	arg1	beer					405:408	barley malt beer	393:408	barley malt beer	393:408	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	7	56	theme	dextrin	993:999	arg1	different					1055:1063	different	1055:1063	different	1055:1063	The dextrin content and NSP content were significantly (P < 0.05) different in wheat beer and barley malt beer, and their content was significantly (P < 0.01) correlated with the content of extracts in beer.
32077484	7	56	theme	dextrin	993:999	arg1	content					1001:1007	The dextrin content	989:1007	The dextrin content	989:1007	The dextrin content and NSP content were significantly (P < 0.05) different in wheat beer and barley malt beer, and their content was significantly (P < 0.01) correlated with the content of extracts in beer.
32077484	6	57	from	carbohydrates	952:964	arg1	beer					969:972	beer	969:972	beer	969:972	Dextrin, oligosaccharides with 2-6 polymerization degree and non-starch polysaccharides (NSP) make up 15.90-34.83%, 17.59-38.63%, and 2.33-7.47% of the total carbohydrates in beer, respectively.
32077484	9	58	theme	principal	1402:1410	arg1	analysis					1422:1429	principal component analysis	1402:1429	principal component analysis	1402:1429	These beer samples could be categorized clearly into three groups by principal component analysis.
32077484	6	59	theme	carbohydrates	952:964	arg1	carbohydrates					952:964	the total carbohydrates	942:964	the total carbohydrates in beer	942:972	Dextrin, oligosaccharides with 2-6 polymerization degree and non-starch polysaccharides (NSP) make up 15.90-34.83%, 17.59-38.63%, and 2.33-7.47% of the total carbohydrates in beer, respectively.
32077484	6	59	theme	carbohydrates	952:964	arg1	%					937:937	2.33-7.47%	928:937	2.33-7.47% of the total carbohydrates in beer	928:972	Dextrin, oligosaccharides with 2-6 polymerization degree and non-starch polysaccharides (NSP) make up 15.90-34.83%, 17.59-38.63%, and 2.33-7.47% of the total carbohydrates in beer, respectively.
32077484	6	59	theme	carbohydrates	952:964	arg1	%					907:907	15.90-34.83%	896:907	15.90-34.83%	896:907	Dextrin, oligosaccharides with 2-6 polymerization degree and non-starch polysaccharides (NSP) make up 15.90-34.83%, 17.59-38.63%, and 2.33-7.47% of the total carbohydrates in beer, respectively.
32077484	6	59	theme	carbohydrates	952:964	arg1	%					921:921	17.59-38.63%	910:921	17.59-38.63%	910:921	Dextrin, oligosaccharides with 2-6 polymerization degree and non-starch polysaccharides (NSP) make up 15.90-34.83%, 17.59-38.63%, and 2.33-7.47% of the total carbohydrates in beer, respectively.
32077484	2	60	theme	sensory	234:240	arg1	characteristics					242:256	the sensory characteristics	230:256	the sensory characteristics of the beer and so their determination is of great interest	230:316	Any change in their composition may influence the sensory characteristics of the beer and so their determination is of great interest.
32077484	1	61	theme	important	129:137	arg1	role					139:142	an important role	126:142	an important role	126:142	BACKGROUND The carbohydrates in beer play an important role as they are essential for fermentation.
32077484	8	62	theme	Non-starch	1197:1206	arg1	trisaccharide					1233:1245	Non-starch polysaccharide, dextrin, trisaccharide, and tetrasaccharide content	1197:1274	trisaccharide	1233:1245	Non-starch polysaccharide, dextrin, trisaccharide, and tetrasaccharide content significantly (P < 0.05) correlated with beer viscosity.
32077484	3	63	from	influence	479:487	arg1	quality					497:503	beer quality	492:503	beer quality	492:503	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	1	64	dep	BACKGROUND	84:93	arg1	play					121:124	play	121:124	play an important role as they are essential for fermentation	121:181	BACKGROUND The carbohydrates in beer play an important role as they are essential for fermentation.
32077484	7	65	from	beer	1074:1077	arg1	different					1055:1063	different	1055:1063	different	1055:1063	The dextrin content and NSP content were significantly (P < 0.05) different in wheat beer and barley malt beer, and their content was significantly (P < 0.01) correlated with the content of extracts in beer.
32077484	7	65	from	beer	1074:1077	arg1	content					1001:1007	The dextrin content	989:1007	The dextrin content	989:1007	The dextrin content and NSP content were significantly (P < 0.05) different in wheat beer and barley malt beer, and their content was significantly (P < 0.01) correlated with the content of extracts in beer.
32077484	5	66	from	present	768:774	arg1	amounts					785:791	small amounts	779:791	small amounts	779:791	Monosaccharides were only present in small amounts.
32077484	0	67	from	beers	41:45	arg1	Profiling					0:8	Profiling	0:8	Profiling of carbohydrates in commercial beers	0:45	Profiling of carbohydrates in commercial beers and their influence on beer quality.
32077484	0	67	from	beers	41:45	arg1	influence					57:65	their influence	51:65	their influence on beer quality	51:81	Profiling of carbohydrates in commercial beers and their influence on beer quality.
32077484	3	68	theme	wheat	411:415	arg1	types					366:370	three types	360:370	three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts -	360:458	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	3	68	theme	wheat	411:415	arg1	beer					417:420	wheat beer	411:420	wheat beer	411:420	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	7	69	theme	barley	1083:1088	arg1	beer					1095:1098	barley malt beer	1083:1098	barley malt beer	1083:1098	The dextrin content and NSP content were significantly (P < 0.05) different in wheat beer and barley malt beer, and their content was significantly (P < 0.01) correlated with the content of extracts in beer.
32077484	8	70	theme	tetrasaccharide	1252:1266	arg1	content					1268:1274	Non-starch polysaccharide, dextrin, trisaccharide, and tetrasaccharide content	1197:1274	content	1268:1274	Non-starch polysaccharide, dextrin, trisaccharide, and tetrasaccharide content significantly (P < 0.05) correlated with beer viscosity.
32077484	6	71	from	%	907:907	arg1	beer					969:972	beer	969:972	beer	969:972	Dextrin, oligosaccharides with 2-6 polymerization degree and non-starch polysaccharides (NSP) make up 15.90-34.83%, 17.59-38.63%, and 2.33-7.47% of the total carbohydrates in beer, respectively.
32077484	3	72	theme	quality	590:596	arg1	control					598:604	quality control	590:604	quality control	590:604	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	3	73	theme	barley	427:432	arg1	types					366:370	three types	360:370	three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts -	360:458	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	3	73	theme	barley	427:432	arg1	beer					439:442	barley malt beer	427:442	barley malt beer	427:442	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	10	74	from	oligosaccharides	1447:1462	arg1	beer					1467:1470	beer	1467:1470	beer	1467:1470	CONCLUSION The oligosaccharides in beer reflect yeast utilization, depending on the type of beer.
32077484	7	75	theme	extracts	1179:1186	arg1	content					1168:1174	the content	1164:1174	the content of extracts in beer	1164:1194	The dextrin content and NSP content were significantly (P < 0.05) different in wheat beer and barley malt beer, and their content was significantly (P < 0.01) correlated with the content of extracts in beer.
32077484	3	76	theme	barley	393:398	arg1	types					366:370	three types	360:370	three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts -	360:458	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	3	76	theme	barley	393:398	arg1	beer					405:408	barley malt beer	393:408	barley malt beer	393:408	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	12	77	theme	different	1758:1766	arg1	types					1773:1777	different beer types	1758:1777	different beer types	1758:1777	Their composition and concentration influenced its characteristics and quality, and played an important role in the discrimination of different beer types.
32077484	6	78	with	polysaccharides	866:880	arg1	degree					844:849	2-6 polymerization degree	825:849	2-6 polymerization degree	825:849	Dextrin, oligosaccharides with 2-6 polymerization degree and non-starch polysaccharides (NSP) make up 15.90-34.83%, 17.59-38.63%, and 2.33-7.47% of the total carbohydrates in beer, respectively.
32077484	3	79	theme	beer	386:389	arg1	types					366:370	three types	360:370	three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts -	360:458	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	3	79	theme	beer	386:389	arg1	beer					439:442	barley malt beer	427:442	barley malt beer	427:442	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	3	79	theme	beer	386:389	arg1	beer					417:420	wheat beer	411:420	wheat beer	411:420	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	3	79	theme	beer	386:389	arg1	beer					405:408	barley malt beer	393:408	barley malt beer	393:408	This study compares the carbohydrates in three types of commercial beer - barley malt beer, wheat beer, and barley malt beer with adjuncts - and examines their influence on beer quality, which is important for selecting raw ingredients and production conditions, and for quality control.
32077484	12	80	theme	types	1773:1777	arg1	discrimination					1740:1753	the discrimination	1736:1753	the discrimination of different beer types	1736:1777	Their composition and concentration influenced its characteristics and quality, and played an important role in the discrimination of different beer types.
32077484	8	81	theme	beer	1317:1320	arg1	viscosity					1322:1330	beer viscosity	1317:1330	beer viscosity	1317:1330	Non-starch polysaccharide, dextrin, trisaccharide, and tetrasaccharide content significantly (P < 0.05) correlated with beer viscosity.
32077484	2	82	theme	great	303:307	arg1	interest					309:316	great interest	303:316	great interest	303:316	Any change in their composition may influence the sensory characteristics of the beer and so their determination is of great interest.
32077484	11	83	with	NSP	1585:1587	arg1	polymerization					1565:1578	2-4 polymerization	1561:1578	2-4 polymerization	1561:1578	Dextrin, oligosaccharides with 2-4 polymerization, and NSP, were major carbohydrates in beer.
32077484	11	84	with	Dextrin	1530:1536	arg1	polymerization					1565:1578	2-4 polymerization	1561:1578	2-4 polymerization	1561:1578	Dextrin, oligosaccharides with 2-4 polymerization, and NSP, were major carbohydrates in beer.
32077484	11	85	with	oligosaccharides	1539:1554	arg1	polymerization					1565:1578	2-4 polymerization	1561:1578	2-4 polymerization	1561:1578	Dextrin, oligosaccharides with 2-4 polymerization, and NSP, were major carbohydrates in beer.
33618343	2	0	used	utilized	230:237	arg2	It					212:213	It	212:213	It	212:213	It is extensively utilized in bone scaffold synthesis due to its better biological system activity and biocompatibility.
33618343	3	1	theme	tricalcium	350:359	arg1	UTCP					372:375	UTCP	372:375	UTCP	372:375	Here, ultra-long tricalcium phosphate (UTCP) was synthesized with the assistance of the ultrasonication method.
33618343	3	1	theme	tricalcium	350:359	arg1	phosphate					361:369	ultra-long tricalcium phosphate	339:369	ultra-long tricalcium phosphate (UTCP)	339:376	Here, ultra-long tricalcium phosphate (UTCP) was synthesized with the assistance of the ultrasonication method.
33618343	10	2	theme	viability	1335:1343	arg1	behavior					1345:1352	good viability behavior	1330:1352	good viability behavior up to 96.0%	1330:1364	The UTCP/MAC and UTCP/MAC/MTX's osteoblast-like (MG-63) cell viability was investigated, and the MTX-loaded UTCP/MAC composite exhibits good viability behavior up to 96.0% in 14 d.
33618343	1	3	theme	bone	182:185	arg1	repair					187:192	bone repair	182:192	bone repair	182:192	Bioceramic morphology plays a crucial role in bone repair and regeneration.
33618343	6	4	dep	show	768:771	arg1	altered					807:813	altered	807:813	show the UTCP crystalline phase is not altered after the reinforcement of the MAC polymer and loading of MTX drugs	768:881	The crystalline natures are investigated by x-ray diffraction, and the results show the UTCP crystalline phase is not altered after the reinforcement of the MAC polymer and loading of MTX drugs.
33618343	12	5	theme	bone	1488:1491	arg1	preparation					1506:1516	bone implantation preparation	1488:1516	bone implantation preparation	1488:1516	It may be suitable for bone implantation preparation, and it helps in faster regeneration of bone tissue afterin vivoand clinical evaluation.
33618343	4	6	theme	chitosan	520:527	arg1	polymer					535:541	a methacrylate chitosan (MAC) polymer	505:541	a methacrylate chitosan (MAC) polymer	505:541	The UTCP was modified as a scaffold by the reinforcement of a methacrylate chitosan (MAC) polymer.
33618343	4	7	theme	MAC	530:532	arg1	polymer					535:541	a methacrylate chitosan (MAC) polymer	505:541	a methacrylate chitosan (MAC) polymer	505:541	The UTCP was modified as a scaffold by the reinforcement of a methacrylate chitosan (MAC) polymer.
33618343	7	8	theme	polymer-coated	968:981	arg1	structures					987:996	polymer-coated rod structures	968:996	polymer-coated rod structures	968:996	The morphological analyses were observed through electron microscopic analysis, and polymer-coated rod structures were observed.
33618343	10	9	theme	osteoblast-like	1226:1240	arg1	viability					1255:1263	UTCP/MAC/MTX's osteoblast-like (MG-63) cell viability	1211:1263	UTCP/MAC/MTX's osteoblast-like (MG-63) cell viability	1211:1263	The UTCP/MAC and UTCP/MAC/MTX's osteoblast-like (MG-63) cell viability was investigated, and the MTX-loaded UTCP/MAC composite exhibits good viability behavior up to 96.0% in 14 d.
33618343	0	10	theme	bone	117:120	arg1	regeneration					122:133	bone regeneration	117:133	bone regeneration	117:133	Osteoblast cell viability over ultra-long tricalcium phosphate nanocrystal-based methacrylate chitosan composite for bone regeneration.
33618343	6	11	theme	UTCP	777:780	arg1	phase					794:798	the UTCP crystalline phase	773:798	the UTCP crystalline phase	773:798	The crystalline natures are investigated by x-ray diffraction, and the results show the UTCP crystalline phase is not altered after the reinforcement of the MAC polymer and loading of MTX drugs.
33618343	5	12	theme	-loaded	603:609	arg1	composites					611:620	UTCP, UTCP/MAC, and methotrexate (MTX)-loaded composites	565:620	UTCP, UTCP/MAC, and methotrexate (MTX)-loaded composites	565:620	The functionality of UTCP, UTCP/MAC, and methotrexate (MTX)-loaded composites was characterized through Fourier transform infrared spectroscopy.
33618343	9	13	from	h	1162:1162	arg1	%					1154:1154	76.15%	1149:1154	76.15% at 20 h	1149:1162	MTX loading and release at 79.0% within 3 h and 76.15% at 20 h, respectively, were achieved.
33618343	9	13	from	h	1162:1162	arg1	%					1132:1132	79.0%	1128:1132	79.0% within 3 h	1128:1143	MTX loading and release at 79.0% within 3 h and 76.15% at 20 h, respectively, were achieved.
33618343	10	14	dep	behavior	1345:1352	arg1	%					1364:1364	%	1364:1364	%	1364:1364	The UTCP/MAC and UTCP/MAC/MTX's osteoblast-like (MG-63) cell viability was investigated, and the MTX-loaded UTCP/MAC composite exhibits good viability behavior up to 96.0% in 14 d.
33618343	12	15	theme	implantation	1493:1504	arg1	preparation					1506:1516	bone implantation preparation	1488:1516	bone implantation preparation	1488:1516	It may be suitable for bone implantation preparation, and it helps in faster regeneration of bone tissue afterin vivoand clinical evaluation.
33618343	11	16	theme	higher	1399:1404	arg1	compatibility					1406:1418	the higher compatibility	1395:1418	the higher compatibility of the composite and profitable cell growth	1395:1462	The results confirm the higher compatibility of the composite and profitable cell growth.
33618343	5	17	theme	composites	611:620	arg1	functionality					548:560	The functionality	544:560	The functionality of UTCP, UTCP/MAC, and methotrexate (MTX)-loaded composites	544:620	The functionality of UTCP, UTCP/MAC, and methotrexate (MTX)-loaded composites was characterized through Fourier transform infrared spectroscopy.
33618343	6	18	theme	x-ray	733:737	arg1	diffraction					739:749	x-ray diffraction	733:749	x-ray diffraction	733:749	The crystalline natures are investigated by x-ray diffraction, and the results show the UTCP crystalline phase is not altered after the reinforcement of the MAC polymer and loading of MTX drugs.
33618343	8	19	dep	Pa.	1097:1099	arg1	to					1089:1090	to	1089:1090	to	1089:1090	The UTCP/MAC composite mechanical stress was increased from 1813 Pa of UTCP to 4272 Pa.
33618343	4	20	theme	methacrylate	507:518	arg1	polymer					535:541	a methacrylate chitosan (MAC) polymer	505:541	a methacrylate chitosan (MAC) polymer	505:541	The UTCP was modified as a scaffold by the reinforcement of a methacrylate chitosan (MAC) polymer.
33618343	8	21	theme	mechanical	1036:1045	arg1	stress					1047:1052	The UTCP/MAC composite mechanical stress	1013:1052	The UTCP/MAC composite mechanical stress	1013:1052	The UTCP/MAC composite mechanical stress was increased from 1813 Pa of UTCP to 4272 Pa.
33618343	2	22	theme	system	295:300	arg1	activity					302:309	its better biological system activity	273:309	its better biological system activity	273:309	It is extensively utilized in bone scaffold synthesis due to its better biological system activity and biocompatibility.
33618343	0	23	theme	cell	11:14	arg1	viability					16:24	Osteoblast cell viability	0:24	Osteoblast cell viability over ultra-long tricalcium phosphate	0:61	Osteoblast cell viability over ultra-long tricalcium phosphate nanocrystal-based methacrylate chitosan composite for bone regeneration.
33618343	12	24	theme	afterin	1570:1576	arg1	evaluation					1595:1604	bone tissue afterin vivoand clinical evaluation	1558:1604	bone tissue afterin vivoand clinical evaluation	1558:1604	It may be suitable for bone implantation preparation, and it helps in faster regeneration of bone tissue afterin vivoand clinical evaluation.
33618343	8	25	theme	composite	1026:1034	arg1	stress					1047:1052	The UTCP/MAC composite mechanical stress	1013:1052	The UTCP/MAC composite mechanical stress	1013:1052	The UTCP/MAC composite mechanical stress was increased from 1813 Pa of UTCP to 4272 Pa.
33618343	10	26	theme	cell	1250:1253	arg1	viability					1255:1263	UTCP/MAC/MTX's osteoblast-like (MG-63) cell viability	1211:1263	UTCP/MAC/MTX's osteoblast-like (MG-63) cell viability	1211:1263	The UTCP/MAC and UTCP/MAC/MTX's osteoblast-like (MG-63) cell viability was investigated, and the MTX-loaded UTCP/MAC composite exhibits good viability behavior up to 96.0% in 14 d.
33618343	0	27	theme	Osteoblast	0:9	arg1	viability					16:24	Osteoblast cell viability	0:24	Osteoblast cell viability over ultra-long tricalcium phosphate	0:61	Osteoblast cell viability over ultra-long tricalcium phosphate nanocrystal-based methacrylate chitosan composite for bone regeneration.
33618343	12	28	theme	tissue	1563:1568	arg1	evaluation					1595:1604	bone tissue afterin vivoand clinical evaluation	1558:1604	bone tissue afterin vivoand clinical evaluation	1558:1604	It may be suitable for bone implantation preparation, and it helps in faster regeneration of bone tissue afterin vivoand clinical evaluation.
33618343	5	29	theme	methotrexate	585:596	arg1	composites					611:620	UTCP, UTCP/MAC, and methotrexate (MTX)-loaded composites	565:620	UTCP, UTCP/MAC, and methotrexate (MTX)-loaded composites	565:620	The functionality of UTCP, UTCP/MAC, and methotrexate (MTX)-loaded composites was characterized through Fourier transform infrared spectroscopy.
33618343	10	30	theme	MTX-loaded	1291:1300	arg1	composite					1311:1319	the MTX-loaded UTCP/MAC composite	1287:1319	the MTX-loaded UTCP/MAC composite	1287:1319	The UTCP/MAC and UTCP/MAC/MTX's osteoblast-like (MG-63) cell viability was investigated, and the MTX-loaded UTCP/MAC composite exhibits good viability behavior up to 96.0% in 14 d.
33618343	9	31	from	%	1154:1154	arg1	loading					1105:1111	MTX loading	1101:1111	MTX loading	1101:1111	MTX loading and release at 79.0% within 3 h and 76.15% at 20 h, respectively, were achieved.
33618343	9	31	from	%	1154:1154	arg1	release					1117:1123	release	1117:1123	release	1117:1123	MTX loading and release at 79.0% within 3 h and 76.15% at 20 h, respectively, were achieved.
33618343	5	32	dep	transform	656:664	arg1	infrared					666:673	infrared	666:673	transform infrared spectroscopy	656:686	The functionality of UTCP, UTCP/MAC, and methotrexate (MTX)-loaded composites was characterized through Fourier transform infrared spectroscopy.
33618343	7	33	theme	microscopic	942:952	arg1	analysis					954:961	electron microscopic analysis	933:961	electron microscopic analysis	933:961	The morphological analyses were observed through electron microscopic analysis, and polymer-coated rod structures were observed.
33618343	4	34	mod	modified	458:465	arg3	reinforcement					488:500	the reinforcement	484:500	the reinforcement of a methacrylate chitosan (MAC) polymer	484:541	The UTCP was modified as a scaffold by the reinforcement of a methacrylate chitosan (MAC) polymer.
33618343	4	34	mod	modified	458:465	arg1	scaffold					472:479	a scaffold	470:479	a scaffold	470:479	The UTCP was modified as a scaffold by the reinforcement of a methacrylate chitosan (MAC) polymer.
33618343	4	34	mod	modified	458:465	arg1	UTCP					449:452	The UTCP	445:452	The UTCP	445:452	The UTCP was modified as a scaffold by the reinforcement of a methacrylate chitosan (MAC) polymer.
33618343	7	35	theme	rod	983:985	arg1	structures					987:996	polymer-coated rod structures	968:996	polymer-coated rod structures	968:996	The morphological analyses were observed through electron microscopic analysis, and polymer-coated rod structures were observed.
33618343	12	36	theme	clinical	1586:1593	arg1	evaluation					1595:1604	bone tissue afterin vivoand clinical evaluation	1558:1604	bone tissue afterin vivoand clinical evaluation	1558:1604	It may be suitable for bone implantation preparation, and it helps in faster regeneration of bone tissue afterin vivoand clinical evaluation.
33618343	12	37	theme	vivoand	1578:1584	arg1	evaluation					1595:1604	bone tissue afterin vivoand clinical evaluation	1558:1604	bone tissue afterin vivoand clinical evaluation	1558:1604	It may be suitable for bone implantation preparation, and it helps in faster regeneration of bone tissue afterin vivoand clinical evaluation.
33618343	8	38	theme	Pa.	1097:1099	arg1	Pa					1078:1079	1813 Pa	1073:1079	1813 Pa of UTCP to 4272 Pa.	1073:1099	The UTCP/MAC composite mechanical stress was increased from 1813 Pa of UTCP to 4272 Pa.
33618343	6	39	theme	drugs	877:881	arg1	reinforcement					825:837	the reinforcement	821:837	the reinforcement of the MAC polymer	821:856	The crystalline natures are investigated by x-ray diffraction, and the results show the UTCP crystalline phase is not altered after the reinforcement of the MAC polymer and loading of MTX drugs.
33618343	6	39	theme	drugs	877:881	arg1	loading					862:868	loading	862:868	loading of MTX drugs	862:881	The crystalline natures are investigated by x-ray diffraction, and the results show the UTCP crystalline phase is not altered after the reinforcement of the MAC polymer and loading of MTX drugs.
33618343	1	40	theme	Bioceramic	136:145	arg1	morphology					147:156	Bioceramic morphology	136:156	Bioceramic morphology	136:156	Bioceramic morphology plays a crucial role in bone repair and regeneration.
33618343	6	41	theme	crystalline	693:703	arg1	natures					705:711	The crystalline natures	689:711	The crystalline natures	689:711	The crystalline natures are investigated by x-ray diffraction, and the results show the UTCP crystalline phase is not altered after the reinforcement of the MAC polymer and loading of MTX drugs.
33618343	11	42	theme	composite	1427:1435	arg1	growth					1457:1462	the composite and profitable cell growth	1423:1462	the composite and profitable cell growth	1423:1462	The results confirm the higher compatibility of the composite and profitable cell growth.
33618343	0	43	theme	tricalcium	42:51	arg1	phosphate					53:61	ultra-long tricalcium phosphate	31:61	ultra-long tricalcium phosphate	31:61	Osteoblast cell viability over ultra-long tricalcium phosphate nanocrystal-based methacrylate chitosan composite for bone regeneration.
33618343	9	44	theme	MTX	1101:1103	arg1	loading					1105:1111	MTX loading	1101:1111	MTX loading	1101:1111	MTX loading and release at 79.0% within 3 h and 76.15% at 20 h, respectively, were achieved.
33618343	6	45	theme	MTX	873:875	arg1	drugs					877:881	MTX drugs	873:881	MTX drugs	873:881	The crystalline natures are investigated by x-ray diffraction, and the results show the UTCP crystalline phase is not altered after the reinforcement of the MAC polymer and loading of MTX drugs.
33618343	0	46	theme	ultra-long	31:40	arg1	phosphate					53:61	ultra-long tricalcium phosphate	31:61	ultra-long tricalcium phosphate	31:61	Osteoblast cell viability over ultra-long tricalcium phosphate nanocrystal-based methacrylate chitosan composite for bone regeneration.
33618343	8	47	theme	UTCP/MAC	1017:1024	arg1	stress					1047:1052	The UTCP/MAC composite mechanical stress	1013:1052	The UTCP/MAC composite mechanical stress	1013:1052	The UTCP/MAC composite mechanical stress was increased from 1813 Pa of UTCP to 4272 Pa.
33618343	5	48	dep	Fourier	648:654	arg1	transform					656:664	transform	656:664	transform infrared spectroscopy	656:686	The functionality of UTCP, UTCP/MAC, and methotrexate (MTX)-loaded composites was characterized through Fourier transform infrared spectroscopy.
33618343	10	49	theme	MG-63	1243:1247	arg1	viability					1255:1263	UTCP/MAC/MTX's osteoblast-like (MG-63) cell viability	1211:1263	UTCP/MAC/MTX's osteoblast-like (MG-63) cell viability	1211:1263	The UTCP/MAC and UTCP/MAC/MTX's osteoblast-like (MG-63) cell viability was investigated, and the MTX-loaded UTCP/MAC composite exhibits good viability behavior up to 96.0% in 14 d.
33618343	9	50	from	%	1132:1132	arg1	loading					1105:1111	MTX loading	1101:1111	MTX loading	1101:1111	MTX loading and release at 79.0% within 3 h and 76.15% at 20 h, respectively, were achieved.
33618343	9	50	from	%	1132:1132	arg1	release					1117:1123	release	1117:1123	release	1117:1123	MTX loading and release at 79.0% within 3 h and 76.15% at 20 h, respectively, were achieved.
33618343	6	51	theme	crystalline	782:792	arg1	phase					794:798	the UTCP crystalline phase	773:798	the UTCP crystalline phase	773:798	The crystalline natures are investigated by x-ray diffraction, and the results show the UTCP crystalline phase is not altered after the reinforcement of the MAC polymer and loading of MTX drugs.
33618343	3	52	theme	ultrasonication	421:435	arg1	method					437:442	the ultrasonication method	417:442	the ultrasonication method	417:442	Here, ultra-long tricalcium phosphate (UTCP) was synthesized with the assistance of the ultrasonication method.
33618343	4	53	theme	polymer	535:541	arg1	reinforcement					488:500	the reinforcement	484:500	the reinforcement of a methacrylate chitosan (MAC) polymer	484:541	The UTCP was modified as a scaffold by the reinforcement of a methacrylate chitosan (MAC) polymer.
33618343	12	54	theme	evaluation	1595:1604	arg1	regeneration					1542:1553	faster regeneration	1535:1553	faster regeneration of bone tissue afterin vivoand clinical evaluation	1535:1604	It may be suitable for bone implantation preparation, and it helps in faster regeneration of bone tissue afterin vivoand clinical evaluation.
33618343	12	55	theme	bone	1558:1561	arg1	evaluation					1595:1604	bone tissue afterin vivoand clinical evaluation	1558:1604	bone tissue afterin vivoand clinical evaluation	1558:1604	It may be suitable for bone implantation preparation, and it helps in faster regeneration of bone tissue afterin vivoand clinical evaluation.
33618343	2	56	theme	scaffold	247:254	arg1	synthesis					256:264	bone scaffold synthesis	242:264	bone scaffold synthesis due to its better biological system activity and biocompatibility	242:330	It is extensively utilized in bone scaffold synthesis due to its better biological system activity and biocompatibility.
33618343	5	57	theme	UTCP	565:568	arg1	composites					611:620	UTCP, UTCP/MAC, and methotrexate (MTX)-loaded composites	565:620	UTCP, UTCP/MAC, and methotrexate (MTX)-loaded composites	565:620	The functionality of UTCP, UTCP/MAC, and methotrexate (MTX)-loaded composites was characterized through Fourier transform infrared spectroscopy.
33618343	3	58	theme	method	437:442	arg1	assistance					403:412	the assistance	399:412	the assistance of the ultrasonication method	399:442	Here, ultra-long tricalcium phosphate (UTCP) was synthesized with the assistance of the ultrasonication method.
33618343	3	59	theme	ultra-long	339:348	arg1	UTCP					372:375	UTCP	372:375	UTCP	372:375	Here, ultra-long tricalcium phosphate (UTCP) was synthesized with the assistance of the ultrasonication method.
33618343	3	59	theme	ultra-long	339:348	arg1	phosphate					361:369	ultra-long tricalcium phosphate	339:369	ultra-long tricalcium phosphate (UTCP)	339:376	Here, ultra-long tricalcium phosphate (UTCP) was synthesized with the assistance of the ultrasonication method.
33618343	12	60	theme	faster	1535:1540	arg1	regeneration					1542:1553	faster regeneration	1535:1553	faster regeneration of bone tissue afterin vivoand clinical evaluation	1535:1604	It may be suitable for bone implantation preparation, and it helps in faster regeneration of bone tissue afterin vivoand clinical evaluation.
33618343	2	61	theme	bone	242:245	arg1	synthesis					256:264	bone scaffold synthesis	242:264	bone scaffold synthesis due to its better biological system activity and biocompatibility	242:330	It is extensively utilized in bone scaffold synthesis due to its better biological system activity and biocompatibility.
33618343	11	62	theme	growth	1457:1462	arg1	compatibility					1406:1418	the higher compatibility	1395:1418	the higher compatibility of the composite and profitable cell growth	1395:1462	The results confirm the higher compatibility of the composite and profitable cell growth.
33618343	10	63	dep	96.0	1360:1363	arg1	to					1357:1358	to	1357:1358	to	1357:1358	The UTCP/MAC and UTCP/MAC/MTX's osteoblast-like (MG-63) cell viability was investigated, and the MTX-loaded UTCP/MAC composite exhibits good viability behavior up to 96.0% in 14 d.
33618343	6	64	theme	polymer	850:856	arg1	reinforcement					825:837	the reinforcement	821:837	the reinforcement of the MAC polymer	821:856	The crystalline natures are investigated by x-ray diffraction, and the results show the UTCP crystalline phase is not altered after the reinforcement of the MAC polymer and loading of MTX drugs.
33618343	6	64	theme	polymer	850:856	arg1	loading					862:868	loading	862:868	loading of MTX drugs	862:881	The crystalline natures are investigated by x-ray diffraction, and the results show the UTCP crystalline phase is not altered after the reinforcement of the MAC polymer and loading of MTX drugs.
33618343	2	65	theme	biological	284:293	arg1	activity					302:309	its better biological system activity	273:309	its better biological system activity	273:309	It is extensively utilized in bone scaffold synthesis due to its better biological system activity and biocompatibility.
33618343	5	66	theme	UTCP/MAC	571:578	arg1	composites					611:620	UTCP, UTCP/MAC, and methotrexate (MTX)-loaded composites	565:620	UTCP, UTCP/MAC, and methotrexate (MTX)-loaded composites	565:620	The functionality of UTCP, UTCP/MAC, and methotrexate (MTX)-loaded composites was characterized through Fourier transform infrared spectroscopy.
33618343	7	67	theme	electron	933:940	arg1	analysis					954:961	electron microscopic analysis	933:961	electron microscopic analysis	933:961	The morphological analyses were observed through electron microscopic analysis, and polymer-coated rod structures were observed.
33618343	10	68	theme	UTCP/MAC	1302:1309	arg1	composite					1311:1319	the MTX-loaded UTCP/MAC composite	1287:1319	the MTX-loaded UTCP/MAC composite	1287:1319	The UTCP/MAC and UTCP/MAC/MTX's osteoblast-like (MG-63) cell viability was investigated, and the MTX-loaded UTCP/MAC composite exhibits good viability behavior up to 96.0% in 14 d.
33618343	6	69	theme	MAC	846:848	arg1	polymer					850:856	the MAC polymer	842:856	the MAC polymer	842:856	The crystalline natures are investigated by x-ray diffraction, and the results show the UTCP crystalline phase is not altered after the reinforcement of the MAC polymer and loading of MTX drugs.
33618343	2	70	theme	better	277:282	arg1	activity					302:309	its better biological system activity	273:309	its better biological system activity	273:309	It is extensively utilized in bone scaffold synthesis due to its better biological system activity and biocompatibility.
33618343	1	71	theme	crucial	166:172	arg1	role					174:177	a crucial role	164:177	a crucial role	164:177	Bioceramic morphology plays a crucial role in bone repair and regeneration.
33618343	10	72	theme	good	1330:1333	arg1	behavior					1345:1352	good viability behavior	1330:1352	good viability behavior up to 96.0%	1330:1364	The UTCP/MAC and UTCP/MAC/MTX's osteoblast-like (MG-63) cell viability was investigated, and the MTX-loaded UTCP/MAC composite exhibits good viability behavior up to 96.0% in 14 d.
33618343	11	73	theme	profitable	1441:1450	arg1	growth					1457:1462	the composite and profitable cell growth	1423:1462	the composite and profitable cell growth	1423:1462	The results confirm the higher compatibility of the composite and profitable cell growth.
33618343	0	74	theme	chitosan	94:101	arg1	composite					103:111	methacrylate chitosan composite	81:111	methacrylate chitosan composite	81:111	Osteoblast cell viability over ultra-long tricalcium phosphate nanocrystal-based methacrylate chitosan composite for bone regeneration.
33618343	10	75	dep	%	1364:1364	arg1	96.0					1360:1363	96.0	1360:1363	96.0	1360:1363	The UTCP/MAC and UTCP/MAC/MTX's osteoblast-like (MG-63) cell viability was investigated, and the MTX-loaded UTCP/MAC composite exhibits good viability behavior up to 96.0% in 14 d.
33618343	11	76	theme	cell	1452:1455	arg1	growth					1457:1462	the composite and profitable cell growth	1423:1462	the composite and profitable cell growth	1423:1462	The results confirm the higher compatibility of the composite and profitable cell growth.
33618343	0	77	theme	methacrylate	81:92	arg1	composite					103:111	methacrylate chitosan composite	81:111	methacrylate chitosan composite	81:111	Osteoblast cell viability over ultra-long tricalcium phosphate nanocrystal-based methacrylate chitosan composite for bone regeneration.
33618343	7	78	theme	morphological	888:900	arg1	analyses					902:909	The morphological analyses	884:909	The morphological analyses	884:909	The morphological analyses were observed through electron microscopic analysis, and polymer-coated rod structures were observed.
34033886	0	0	theme	constipation	79:90	arg1	improvement					53:63	improvement	53:63	improvement of functional constipation by Chrysanthemum morifolium polysaccharide	53:133	The effect of microbial composition and proteomic on improvement of functional constipation by Chrysanthemum morifolium polysaccharide.
34033886	1	1	theme	improving	354:362	arg1	constipation					364:375	improving constipation	354:375	improving constipation	354:375	In this paper, SD rat constipation model was established with loperamide hydrochloride to study the effect of Chrysanthemum morifolium polysaccharide on the improvement of functional constipation, and the mechanism of improving constipation was investigated with the proteomics and intestinal flora.
34033886	2	2	theme	intestine	586:594	arg1	rate					607:610	small intestine propulsion rate	580:610	small intestine propulsion rate	580:610	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	1	3	theme	rat	154:156	arg1	model					171:175	SD rat constipation model	151:175	SD rat constipation model	151:175	In this paper, SD rat constipation model was established with loperamide hydrochloride to study the effect of Chrysanthemum morifolium polysaccharide on the improvement of functional constipation, and the mechanism of improving constipation was investigated with the proteomics and intestinal flora.
34033886	1	4	theme	morifolium	260:269	arg1	polysaccharide					271:284	Chrysanthemum morifolium polysaccharide	246:284	Chrysanthemum morifolium polysaccharide	246:284	In this paper, SD rat constipation model was established with loperamide hydrochloride to study the effect of Chrysanthemum morifolium polysaccharide on the improvement of functional constipation, and the mechanism of improving constipation was investigated with the proteomics and intestinal flora.
34033886	0	5	theme	functional	68:77	arg1	constipation					79:90	functional constipation	68:90	functional constipation	68:90	The effect of microbial composition and proteomic on improvement of functional constipation by Chrysanthemum morifolium polysaccharide.
34033886	0	6	from	effect	4:9	arg1	improvement					53:63	improvement	53:63	improvement of functional constipation by Chrysanthemum morifolium polysaccharide	53:133	The effect of microbial composition and proteomic on improvement of functional constipation by Chrysanthemum morifolium polysaccharide.
34033886	2	7	theme	P	656:656	arg1	levels					538:543	the levels	534:543	the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP)	534:661	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	1	8	theme	constipation	158:169	arg1	model					171:175	SD rat constipation model	151:175	SD rat constipation model	151:175	In this paper, SD rat constipation model was established with loperamide hydrochloride to study the effect of Chrysanthemum morifolium polysaccharide on the improvement of functional constipation, and the mechanism of improving constipation was investigated with the proteomics and intestinal flora.
34033886	1	9	theme	polysaccharide	271:284	arg1	effect					236:241	the effect	232:241	the effect of Chrysanthemum morifolium polysaccharide on the improvement of functional constipation	232:330	In this paper, SD rat constipation model was established with loperamide hydrochloride to study the effect of Chrysanthemum morifolium polysaccharide on the improvement of functional constipation, and the mechanism of improving constipation was investigated with the proteomics and intestinal flora.
34033886	0	10	theme	Chrysanthemum	95:107	arg1	polysaccharide					120:133	Chrysanthemum morifolium polysaccharide	95:133	Chrysanthemum morifolium polysaccharide	95:133	The effect of microbial composition and proteomic on improvement of functional constipation by Chrysanthemum morifolium polysaccharide.
34033886	2	11	theme	substance	646:654	arg1	SP					659:660	SP	659:660	SP	659:660	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	2	11	theme	substance	646:654	arg1	P					656:656	substance P	646:656	substance P (SP)	646:661	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	2	12	theme	growth	686:691	arg1	inhibitor					693:701	growth inhibitor	686:701	growth inhibitor (SS)	686:706	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	2	12	theme	growth	686:691	arg1	SS					704:705	SS	704:705	SS	704:705	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	2	13	theme	content	554:560	arg1	levels					538:543	the levels	534:543	the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP)	534:661	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	2	14	theme	gastrin	613:619	arg1	levels					538:543	the levels	534:543	the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP)	534:661	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	2	15	theme	gastrointestinal	721:736	arg1	motility					738:745	gastrointestinal motility	721:745	gastrointestinal motility	721:745	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	3	16	theme	C.	790:791	arg1	polysaccharide					804:817	C. morifolium polysaccharide	790:817	C. morifolium polysaccharide	790:817	Gut microbial studies showed that C. morifolium polysaccharide could significantly increase species abundance and flora diversity and improve flora structure.
34033886	2	17	theme	water	548:552	arg1	content					554:560	water content	548:560	water content of stool pellets	548:577	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	3	18	theme	flora	898:902	arg1	structure					904:912	flora structure	898:912	flora structure	898:912	Gut microbial studies showed that C. morifolium polysaccharide could significantly increase species abundance and flora diversity and improve flora structure.
34033886	5	19	theme	intestinal	1260:1269	arg1	motility					1271:1278	intestinal motility	1260:1278	intestinal motility	1260:1278	Proteomics studies suggested that C. morifolium polysaccharides could reduce intestinal lesions, enhance intestinal homeostasis, increase amino acid uptake, promote intestinal motility and relieve constipation by regulating the expression of RAS, FABP1 and SLC1A5 proteins.
34033886	0	20	theme	morifolium	109:118	arg1	polysaccharide					120:133	Chrysanthemum morifolium polysaccharide	95:133	Chrysanthemum morifolium polysaccharide	95:133	The effect of microbial composition and proteomic on improvement of functional constipation by Chrysanthemum morifolium polysaccharide.
34033886	5	21	theme	morifolium	1132:1141	arg1	polysaccharides					1143:1157	C. morifolium polysaccharides	1129:1157	C. morifolium polysaccharides	1129:1157	Proteomics studies suggested that C. morifolium polysaccharides could reduce intestinal lesions, enhance intestinal homeostasis, increase amino acid uptake, promote intestinal motility and relieve constipation by regulating the expression of RAS, FABP1 and SLC1A5 proteins.
34033886	3	22	theme	morifolium	793:802	arg1	polysaccharide					804:817	C. morifolium polysaccharide	790:817	C. morifolium polysaccharide	790:817	Gut microbial studies showed that C. morifolium polysaccharide could significantly increase species abundance and flora diversity and improve flora structure.
34033886	5	23	theme	amino	1233:1237	arg1	uptake					1244:1249	amino acid uptake	1233:1249	amino acid uptake	1233:1249	Proteomics studies suggested that C. morifolium polysaccharides could reduce intestinal lesions, enhance intestinal homeostasis, increase amino acid uptake, promote intestinal motility and relieve constipation by regulating the expression of RAS, FABP1 and SLC1A5 proteins.
34033886	4	24	theme	Romboutsia	959:968	arg1	abundance					928:936	The relative abundance	915:936	The relative abundance of Lactobacillus and Romboutsia	915:968	The relative abundance of Lactobacillus and Romboutsia increased, while the relative abundance of Lachnospiraceae_NK4A136_group and Roseburia decreased compared with the MC group.
34033886	2	25	theme	polysaccharide	490:503	arg1	group					467:471	the HD group	460:471	the HD group of C. morifolium polysaccharide	460:503	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	2	25	theme	polysaccharide	490:503	arg1	polysaccharide					490:503	C. morifolium polysaccharide	476:503	C. morifolium polysaccharide	476:503	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	1	26	theme	proteomics	403:412	arg1	flora					429:433	the proteomics and intestinal flora	399:433	flora	429:433	In this paper, SD rat constipation model was established with loperamide hydrochloride to study the effect of Chrysanthemum morifolium polysaccharide on the improvement of functional constipation, and the mechanism of improving constipation was investigated with the proteomics and intestinal flora.
34033886	2	27	theme	gastrin	628:634	arg1	levels					538:543	the levels	534:543	the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP)	534:661	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	5	28	theme	C.	1129:1130	arg1	polysaccharides					1143:1157	C. morifolium polysaccharides	1129:1157	C. morifolium polysaccharides	1129:1157	Proteomics studies suggested that C. morifolium polysaccharides could reduce intestinal lesions, enhance intestinal homeostasis, increase amino acid uptake, promote intestinal motility and relieve constipation by regulating the expression of RAS, FABP1 and SLC1A5 proteins.
34033886	1	29	theme	constipation	364:375	arg1	mechanism					341:349	the mechanism	337:349	the mechanism of improving constipation	337:375	In this paper, SD rat constipation model was established with loperamide hydrochloride to study the effect of Chrysanthemum morifolium polysaccharide on the improvement of functional constipation, and the mechanism of improving constipation was investigated with the proteomics and intestinal flora.
34033886	5	30	theme	Proteomics	1095:1104	arg1	studies					1106:1112	Proteomics studies	1095:1112	Proteomics studies	1095:1112	Proteomics studies suggested that C. morifolium polysaccharides could reduce intestinal lesions, enhance intestinal homeostasis, increase amino acid uptake, promote intestinal motility and relieve constipation by regulating the expression of RAS, FABP1 and SLC1A5 proteins.
34033886	0	31	theme	microbial	14:22	arg1	composition					24:34	microbial composition	14:34	microbial composition	14:34	The effect of microbial composition and proteomic on improvement of functional constipation by Chrysanthemum morifolium polysaccharide.
34033886	2	32	theme	small	580:584	arg1	rate					607:610	small intestine propulsion rate	580:610	small intestine propulsion rate	580:610	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	5	33	theme	SLC1A5	1352:1357	arg1	proteins					1359:1366	SLC1A5 proteins	1352:1366	SLC1A5 proteins	1352:1366	Proteomics studies suggested that C. morifolium polysaccharides could reduce intestinal lesions, enhance intestinal homeostasis, increase amino acid uptake, promote intestinal motility and relieve constipation by regulating the expression of RAS, FABP1 and SLC1A5 proteins.
34033886	1	34	theme	loperamide	198:207	arg1	hydrochloride					209:221	loperamide hydrochloride	198:221	loperamide hydrochloride	198:221	In this paper, SD rat constipation model was established with loperamide hydrochloride to study the effect of Chrysanthemum morifolium polysaccharide on the improvement of functional constipation, and the mechanism of improving constipation was investigated with the proteomics and intestinal flora.
34033886	1	35	theme	functional	308:317	arg1	constipation					319:330	functional constipation	308:330	functional constipation	308:330	In this paper, SD rat constipation model was established with loperamide hydrochloride to study the effect of Chrysanthemum morifolium polysaccharide on the improvement of functional constipation, and the mechanism of improving constipation was investigated with the proteomics and intestinal flora.
34033886	4	36	theme	Roseburia	1047:1055	arg1	abundance					1000:1008	the relative abundance	987:1008	the relative abundance of Lachnospiraceae_NK4A136_group and Roseburia	987:1055	The relative abundance of Lactobacillus and Romboutsia increased, while the relative abundance of Lachnospiraceae_NK4A136_group and Roseburia decreased compared with the MC group.
34033886	4	37	theme	relative	919:926	arg1	abundance					928:936	The relative abundance	915:936	The relative abundance of Lactobacillus and Romboutsia	915:968	The relative abundance of Lactobacillus and Romboutsia increased, while the relative abundance of Lachnospiraceae_NK4A136_group and Roseburia decreased compared with the MC group.
34033886	5	38	theme	proteins	1359:1366	arg1	expression					1323:1332	the expression	1319:1332	the expression of RAS, FABP1 and SLC1A5 proteins	1319:1366	Proteomics studies suggested that C. morifolium polysaccharides could reduce intestinal lesions, enhance intestinal homeostasis, increase amino acid uptake, promote intestinal motility and relieve constipation by regulating the expression of RAS, FABP1 and SLC1A5 proteins.
34033886	5	39	theme	acid	1239:1242	arg1	uptake					1244:1249	amino acid uptake	1233:1249	amino acid uptake	1233:1249	Proteomics studies suggested that C. morifolium polysaccharides could reduce intestinal lesions, enhance intestinal homeostasis, increase amino acid uptake, promote intestinal motility and relieve constipation by regulating the expression of RAS, FABP1 and SLC1A5 proteins.
34033886	1	40	theme	constipation	319:330	arg1	improvement					293:303	the improvement	289:303	the improvement of functional constipation	289:330	In this paper, SD rat constipation model was established with loperamide hydrochloride to study the effect of Chrysanthemum morifolium polysaccharide on the improvement of functional constipation, and the mechanism of improving constipation was investigated with the proteomics and intestinal flora.
34033886	2	41	theme	pellets	571:577	arg1	MTL					622:624	MTL	622:624	MTL	622:624	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	2	41	theme	pellets	571:577	arg1	content					554:560	water content	548:560	water content of stool pellets	548:577	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	2	41	theme	pellets	571:577	arg1	P					656:656	substance P	646:656	substance P (SP)	646:661	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	2	41	theme	pellets	571:577	arg1	rate					607:610	small intestine propulsion rate	580:610	small intestine propulsion rate	580:610	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	2	41	theme	pellets	571:577	arg1	gastrin					628:634	gastrin	628:634	gastrin (GAS)	628:640	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	2	41	theme	pellets	571:577	arg1	GAS					637:639	GAS	637:639	GAS	637:639	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	2	41	theme	pellets	571:577	arg1	SP					659:660	SP	659:660	SP	659:660	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	2	41	theme	pellets	571:577	arg1	gastrin					613:619	gastrin	613:619	gastrin (MTL)	613:625	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	1	42	theme	intestinal	418:427	arg1	flora					429:433	the proteomics and intestinal flora	399:433	flora	429:433	In this paper, SD rat constipation model was established with loperamide hydrochloride to study the effect of Chrysanthemum morifolium polysaccharide on the improvement of functional constipation, and the mechanism of improving constipation was investigated with the proteomics and intestinal flora.
34033886	5	43	theme	FABP1	1342:1346	arg1	expression					1323:1332	the expression	1319:1332	the expression of RAS, FABP1 and SLC1A5 proteins	1319:1366	Proteomics studies suggested that C. morifolium polysaccharides could reduce intestinal lesions, enhance intestinal homeostasis, increase amino acid uptake, promote intestinal motility and relieve constipation by regulating the expression of RAS, FABP1 and SLC1A5 proteins.
34033886	0	44	theme	composition	24:34	arg1	effect					4:9	The effect	0:9	The effect of microbial composition and proteomic on improvement of functional constipation by Chrysanthemum morifolium polysaccharide.	0:134	The effect of microbial composition and proteomic on improvement of functional constipation by Chrysanthemum morifolium polysaccharide.
34033886	2	45	theme	stool	565:569	arg1	pellets					571:577	stool pellets	565:577	stool pellets	565:577	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	2	46	theme	HD	464:465	arg1	group					467:471	the HD group	460:471	the HD group of C. morifolium polysaccharide	460:503	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	2	46	theme	HD	464:465	arg1	polysaccharide					490:503	C. morifolium polysaccharide	476:503	C. morifolium polysaccharide	476:503	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	2	47	theme	morifolium	479:488	arg1	polysaccharide					490:503	C. morifolium polysaccharide	476:503	C. morifolium polysaccharide	476:503	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	4	48	theme	Lactobacillus	941:953	arg1	abundance					928:936	The relative abundance	915:936	The relative abundance of Lactobacillus and Romboutsia	915:968	The relative abundance of Lactobacillus and Romboutsia increased, while the relative abundance of Lachnospiraceae_NK4A136_group and Roseburia decreased compared with the MC group.
34033886	5	49	theme	intestinal	1172:1181	arg1	lesions					1183:1189	intestinal lesions	1172:1189	intestinal lesions	1172:1189	Proteomics studies suggested that C. morifolium polysaccharides could reduce intestinal lesions, enhance intestinal homeostasis, increase amino acid uptake, promote intestinal motility and relieve constipation by regulating the expression of RAS, FABP1 and SLC1A5 proteins.
34033886	0	50	theme	proteomic	40:48	arg1	effect					4:9	The effect	0:9	The effect of microbial composition and proteomic on improvement of functional constipation by Chrysanthemum morifolium polysaccharide.	0:134	The effect of microbial composition and proteomic on improvement of functional constipation by Chrysanthemum morifolium polysaccharide.
34033886	5	51	theme	intestinal	1200:1209	arg1	homeostasis					1211:1221	intestinal homeostasis	1200:1221	intestinal homeostasis	1200:1221	Proteomics studies suggested that C. morifolium polysaccharides could reduce intestinal lesions, enhance intestinal homeostasis, increase amino acid uptake, promote intestinal motility and relieve constipation by regulating the expression of RAS, FABP1 and SLC1A5 proteins.
34033886	2	52	theme	rate	607:610	arg1	levels					538:543	the levels	534:543	the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP)	534:661	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	2	53	theme	C.	476:477	arg1	polysaccharide					490:503	C. morifolium polysaccharide	476:503	C. morifolium polysaccharide	476:503	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	2	54	theme	propulsion	596:605	arg1	rate					607:610	small intestine propulsion rate	580:610	small intestine propulsion rate	580:610	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	4	55	theme	relative	991:998	arg1	abundance					1000:1008	the relative abundance	987:1008	the relative abundance of Lachnospiraceae_NK4A136_group and Roseburia	987:1055	The relative abundance of Lactobacillus and Romboutsia increased, while the relative abundance of Lachnospiraceae_NK4A136_group and Roseburia decreased compared with the MC group.
34033886	3	56	theme	species	848:854	arg1	abundance					856:864	species abundance	848:864	species abundance	848:864	Gut microbial studies showed that C. morifolium polysaccharide could significantly increase species abundance and flora diversity and improve flora structure.
34033886	1	57	from	effect	236:241	arg1	improvement					293:303	the improvement	289:303	the improvement of functional constipation	289:330	In this paper, SD rat constipation model was established with loperamide hydrochloride to study the effect of Chrysanthemum morifolium polysaccharide on the improvement of functional constipation, and the mechanism of improving constipation was investigated with the proteomics and intestinal flora.
34033886	4	58	theme	MC	1085:1086	arg1	group					1088:1092	the MC group	1081:1092	the MC group	1081:1092	The relative abundance of Lactobacillus and Romboutsia increased, while the relative abundance of Lachnospiraceae_NK4A136_group and Roseburia decreased compared with the MC group.
34033886	5	59	theme	RAS	1337:1339	arg1	expression					1323:1332	the expression	1319:1332	the expression of RAS, FABP1 and SLC1A5 proteins	1319:1366	Proteomics studies suggested that C. morifolium polysaccharides could reduce intestinal lesions, enhance intestinal homeostasis, increase amino acid uptake, promote intestinal motility and relieve constipation by regulating the expression of RAS, FABP1 and SLC1A5 proteins.
34033886	3	60	theme	Gut	756:758	arg1	studies					770:776	Gut microbial studies	756:776	Gut microbial studies	756:776	Gut microbial studies showed that C. morifolium polysaccharide could significantly increase species abundance and flora diversity and improve flora structure.
34033886	4	61	theme	Lachnospiraceae_NK4A136_group	1013:1041	arg1	abundance					1000:1008	the relative abundance	987:1008	the relative abundance of Lachnospiraceae_NK4A136_group and Roseburia	987:1055	The relative abundance of Lactobacillus and Romboutsia increased, while the relative abundance of Lachnospiraceae_NK4A136_group and Roseburia decreased compared with the MC group.
34033886	3	62	theme	microbial	760:768	arg1	studies					770:776	Gut microbial studies	756:776	Gut microbial studies	756:776	Gut microbial studies showed that C. morifolium polysaccharide could significantly increase species abundance and flora diversity and improve flora structure.
34033886	2	63	theme	inhibitor	693:701	arg1	level					677:681	the level	673:681	the level of growth inhibitor (SS)	673:706	The results showed that the HD group of C. morifolium polysaccharide could significantly increase the levels of water content of stool pellets, small intestine propulsion rate, gastrin (MTL), gastrin (GAS) and substance P (SP), decrease the level of growth inhibitor (SS) and improved gastrointestinal motility in rats.
34033886	1	64	theme	SD	151:152	arg1	model					171:175	SD rat constipation model	151:175	SD rat constipation model	151:175	In this paper, SD rat constipation model was established with loperamide hydrochloride to study the effect of Chrysanthemum morifolium polysaccharide on the improvement of functional constipation, and the mechanism of improving constipation was investigated with the proteomics and intestinal flora.
34033886	3	65	theme	flora	870:874	arg1	diversity					876:884	flora diversity	870:884	flora diversity	870:884	Gut microbial studies showed that C. morifolium polysaccharide could significantly increase species abundance and flora diversity and improve flora structure.
34033886	1	66	theme	Chrysanthemum	246:258	arg1	polysaccharide					271:284	Chrysanthemum morifolium polysaccharide	246:284	Chrysanthemum morifolium polysaccharide	246:284	In this paper, SD rat constipation model was established with loperamide hydrochloride to study the effect of Chrysanthemum morifolium polysaccharide on the improvement of functional constipation, and the mechanism of improving constipation was investigated with the proteomics and intestinal flora.
32890421	9	0	theme	mature	1565:1570	arg1	grain					1572:1576	mature grain	1565:1576	mature grain	1565:1576	Our data indicate that multiple members of the CslF/H family fulfil important functions during grain development but, with the exception of HvCslF6, do not impact the abundance of (1,3;1,4)-β-glucan in mature grain.
32890421	5	1	dep	-β-Glucan	880:888	arg1	1,3					872:874	1,3	872:874	1,3;1,4	872:878	(1,3;1,4)-β-Glucan was absent in the grain of cslf6 knockout lines, whereas cslf9 knockout lines had similar (1,3;1,4)-β-glucan content to wild-type (WT).
32890421	3	2	theme	genetics	421:428	arg1	approach					430:437	a reverse genetics approach	411:437	a reverse genetics approach	411:437	We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases.
32890421	4	3	theme	stop	761:764	arg1	codons					766:771	premature stop codons	751:771	premature stop codons	751:771	Resultant mutations ranged from single amino acid (aa) substitutions to frameshift mutations causing premature stop codons, and led to specific differences in grain morphology, composition and (1,3;1,4)-β-glucan content.
32890421	9	4	theme	multiple	1386:1393	arg1	members					1395:1401	multiple members	1386:1401	multiple members of the CslF/H family	1386:1422	Our data indicate that multiple members of the CslF/H family fulfil important functions during grain development but, with the exception of HvCslF6, do not impact the abundance of (1,3;1,4)-β-glucan in mature grain.
32890421	5	5	dep	1,3	872:874	arg1	1,4					876:878	1,4	876:878	1,4	876:878	(1,3;1,4)-β-Glucan was absent in the grain of cslf6 knockout lines, whereas cslf9 knockout lines had similar (1,3;1,4)-β-glucan content to wild-type (WT).
32890421	6	6	theme	cslf9	1035:1039	arg1	mutants					1041:1047	cslf9 mutants	1035:1047	cslf9 mutants	1035:1047	However, cslf9 mutants showed changes in the abundance of other cell-wall-related monosaccharides compared with WT.
32890421	1	7	theme	Hordeum	153:159	arg1	vulgare					161:167	Hordeum vulgare	153:167	Hordeum vulgare L	153:169	Barley (Hordeum vulgare L) grain is comparatively rich in (1,3;1,4)-β-glucan, a source of fermentable dietary fibre that protects against various human health conditions.
32890421	4	8	theme	frameshift	722:731	arg1	mutations					733:741	frameshift mutations	722:741	frameshift mutations causing premature stop codons	722:771	Resultant mutations ranged from single amino acid (aa) substitutions to frameshift mutations causing premature stop codons, and led to specific differences in grain morphology, composition and (1,3;1,4)-β-glucan content.
32890421	4	9	dep	1,3	844:846	arg1	1,4					848:850	1,4	848:850	1,3;1,4	844:850	Resultant mutations ranged from single amino acid (aa) substitutions to frameshift mutations causing premature stop codons, and led to specific differences in grain morphology, composition and (1,3;1,4)-β-glucan content.
32890421	4	10	theme	specific	785:792	arg1	differences					794:804	specific differences	785:804	specific differences in grain morphology, composition and (1,3;1,4)-β-glucan content	785:868	Resultant mutations ranged from single amino acid (aa) substitutions to frameshift mutations causing premature stop codons, and led to specific differences in grain morphology, composition and (1,3;1,4)-β-glucan content.
32890421	7	11	theme	grain	1171:1175	arg1	length					1177:1182	grain length	1171:1182	grain length	1171:1182	Thousand grain weight (TGW), grain length, width and surface area were altered in cslf6 knockouts, and to a lesser extent TGW in cslf9 knockouts.
32890421	1	12	from	-β-glucan	212:220	arg1	rich					195:198	rich	195:198	rich	195:198	Barley (Hordeum vulgare L) grain is comparatively rich in (1,3;1,4)-β-glucan, a source of fermentable dietary fibre that protects against various human health conditions.
32890421	8	13	dep	grain	1329:1333	arg1	1,3					1336:1338	1,3	1336:1338	1,3;1,4	1336:1342	cslf3 and cslh1 mutants had no effect on grain (1,3;1,4)-β-glucan content.
32890421	1	14	dep	1,3	204:206	arg1	1,4					208:210	1,4	208:210	1,3;1,4	204:210	Barley (Hordeum vulgare L) grain is comparatively rich in (1,3;1,4)-β-glucan, a source of fermentable dietary fibre that protects against various human health conditions.
32890421	0	15	theme	cell	111:114	arg1	content					136:142	the storage and cell wall polysaccharide content	95:142	content	136:142	Targeted mutation of barley (1,3;1,4)-β-glucan synthases reveals complex relationships between the storage and cell wall polysaccharide content.
32890421	9	16	theme	grain	1458:1462	arg1	development					1464:1474	grain development	1458:1474	grain development	1458:1474	Our data indicate that multiple members of the CslF/H family fulfil important functions during grain development but, with the exception of HvCslF6, do not impact the abundance of (1,3;1,4)-β-glucan in mature grain.
32890421	3	17	theme	-β-glucan	629:637	arg1	synthases					639:647	known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases	557:647	known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases	557:647	We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases.
32890421	3	17	theme	-β-glucan	629:637	arg1	mutations					470:478	mutations	470:478	mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases	470:647	We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases.
32890421	6	18	theme	other	1084:1088	arg1	monosaccharides					1108:1122	other cell-wall-related monosaccharides	1084:1122	other cell-wall-related monosaccharides	1084:1122	However, cslf9 mutants showed changes in the abundance of other cell-wall-related monosaccharides compared with WT.
32890421	0	19	theme	polysaccharide	121:134	arg1	content					136:142	the storage and cell wall polysaccharide content	95:142	content	136:142	Targeted mutation of barley (1,3;1,4)-β-glucan synthases reveals complex relationships between the storage and cell wall polysaccharide content.
32890421	3	20	theme	Cellulose	498:506	arg1	superfamily					533:543	the Cellulose synthase-like (Csl) gene superfamily	494:543	the Cellulose synthase-like (Csl) gene superfamily	494:543	We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases.
32890421	5	21	contain	had	968:970	arg1	lines					962:966	cslf9 knockout lines	947:966	cslf9 knockout lines	947:966	(1,3;1,4)-β-Glucan was absent in the grain of cslf6 knockout lines, whereas cslf9 knockout lines had similar (1,3;1,4)-β-glucan content to wild-type (WT).
32890421	5	21	contain	had	968:970	arg2	content					999:1005	similar (1,3;1,4)-β-glucan content	972:1005	similar (1,3;1,4)-β-glucan content	972:1005	(1,3;1,4)-β-Glucan was absent in the grain of cslf6 knockout lines, whereas cslf9 knockout lines had similar (1,3;1,4)-β-glucan content to wild-type (WT).
32890421	1	22	dep	-β-glucan	212:220	arg1	1,3					204:206	1,3	204:206	1,3;1,4	204:210	Barley (Hordeum vulgare L) grain is comparatively rich in (1,3;1,4)-β-glucan, a source of fermentable dietary fibre that protects against various human health conditions.
32890421	7	23	theme	grain	1151:1155	arg1	TGW					1165:1167	TGW	1165:1167	TGW	1165:1167	Thousand grain weight (TGW), grain length, width and surface area were altered in cslf6 knockouts, and to a lesser extent TGW in cslf9 knockouts.
32890421	7	23	theme	grain	1151:1155	arg1	weight					1157:1162	Thousand grain weight	1142:1162	Thousand grain weight (TGW)	1142:1168	Thousand grain weight (TGW), grain length, width and surface area were altered in cslf6 knockouts, and to a lesser extent TGW in cslf9 knockouts.
32890421	3	24	theme	superfamily	533:543	arg1	members					483:489	members	483:489	members of the Cellulose synthase-like (Csl) gene superfamily	483:543	We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases.
32890421	8	25	contain	had	1312:1314	arg1	mutants					1304:1310	cslf3 and cslh1 mutants	1288:1310	cslf3 and cslh1 mutants	1288:1310	cslf3 and cslh1 mutants had no effect on grain (1,3;1,4)-β-glucan content.
32890421	8	25	contain	had	1312:1314	arg2	effect					1319:1324	no effect	1316:1324	no effect	1316:1324	cslf3 and cslh1 mutants had no effect on grain (1,3;1,4)-β-glucan content.
32890421	3	26	dep	1,3	621:623	arg1	1,4					625:627	1,4	625:627	1,3;1,4	621:627	We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases.
32890421	0	27	theme	synthases	47:55	arg1	mutation					9:16	Targeted mutation	0:16	Targeted mutation of barley (1,3;1,4)-β-glucan synthases	0:55	Targeted mutation of barley (1,3;1,4)-β-glucan synthases reveals complex relationships between the storage and cell wall polysaccharide content.
32890421	1	28	theme	various	283:289	arg1	conditions					304:313	various human health conditions	283:313	various human health conditions	283:313	Barley (Hordeum vulgare L) grain is comparatively rich in (1,3;1,4)-β-glucan, a source of fermentable dietary fibre that protects against various human health conditions.
32890421	1	29	theme	Barley	145:150	arg1	grain					172:176	Barley (Hordeum vulgare L) grain	145:176	Barley (Hordeum vulgare L) grain	145:176	Barley (Hordeum vulgare L) grain is comparatively rich in (1,3;1,4)-β-glucan, a source of fermentable dietary fibre that protects against various human health conditions.
32890421	0	30	theme	complex	65:71	arg1	relationships					73:85	complex relationships	65:85	complex relationships between the storage and cell wall polysaccharide content	65:142	Targeted mutation of barley (1,3;1,4)-β-glucan synthases reveals complex relationships between the storage and cell wall polysaccharide content.
32890421	1	31	theme	health	297:302	arg1	conditions					304:313	various human health conditions	283:313	various human health conditions	283:313	Barley (Hordeum vulgare L) grain is comparatively rich in (1,3;1,4)-β-glucan, a source of fermentable dietary fibre that protects against various human health conditions.
32890421	9	32	theme	HvCslF6	1503:1509	arg1	exception					1490:1498	the exception	1486:1498	the exception of HvCslF6	1486:1509	Our data indicate that multiple members of the CslF/H family fulfil important functions during grain development but, with the exception of HvCslF6, do not impact the abundance of (1,3;1,4)-β-glucan in mature grain.
32890421	4	33	theme	Resultant	650:658	arg1	mutations					660:668	Resultant mutations	650:668	Resultant mutations	650:668	Resultant mutations ranged from single amino acid (aa) substitutions to frameshift mutations causing premature stop codons, and led to specific differences in grain morphology, composition and (1,3;1,4)-β-glucan content.
32890421	5	34	theme	knockout	923:930	arg1	lines					932:936	cslf6 knockout lines	917:936	cslf6 knockout lines	917:936	(1,3;1,4)-β-Glucan was absent in the grain of cslf6 knockout lines, whereas cslf9 knockout lines had similar (1,3;1,4)-β-glucan content to wild-type (WT).
32890421	9	35	dep	fulfil	1424:1429	arg1	impact					1519:1524	impact	1519:1524	impact	1519:1524	Our data indicate that multiple members of the CslF/H family fulfil important functions during grain development but, with the exception of HvCslF6, do not impact the abundance of (1,3;1,4)-β-glucan in mature grain.
32890421	2	36	theme	low	325:327	arg1	content					354:360	low grain (1,3;1,4)-β-glucan content	325:360	low grain (1,3;1,4)-β-glucan content	325:360	However, low grain (1,3;1,4)-β-glucan content is preferred for brewing and distilling.
32890421	0	37	theme	Targeted	0:7	arg1	mutation					9:16	Targeted mutation	0:16	Targeted mutation of barley (1,3;1,4)-β-glucan synthases	0:55	Targeted mutation of barley (1,3;1,4)-β-glucan synthases reveals complex relationships between the storage and cell wall polysaccharide content.
32890421	1	38	dep	vulgare	161:167	arg1	L					169:169	L	169:169	Hordeum vulgare L	153:169	Barley (Hordeum vulgare L) grain is comparatively rich in (1,3;1,4)-β-glucan, a source of fermentable dietary fibre that protects against various human health conditions.
32890421	4	39	theme	acid	695:698	arg1	substitutions					705:717	single amino acid (aa) substitutions	682:717	single amino acid (aa) substitutions	682:717	Resultant mutations ranged from single amino acid (aa) substitutions to frameshift mutations causing premature stop codons, and led to specific differences in grain morphology, composition and (1,3;1,4)-β-glucan content.
32890421	7	40	dep	altered	1213:1219	arg1	TGW					1264:1266	TGW	1264:1266	TGW in cslf9 knockouts	1264:1285	Thousand grain weight (TGW), grain length, width and surface area were altered in cslf6 knockouts, and to a lesser extent TGW in cslf9 knockouts.
32890421	7	41	theme	cslf9	1271:1275	arg1	knockouts					1277:1285	cslf9 knockouts	1271:1285	cslf9 knockouts	1271:1285	Thousand grain weight (TGW), grain length, width and surface area were altered in cslf6 knockouts, and to a lesser extent TGW in cslf9 knockouts.
32890421	4	42	theme	aa	701:702	arg1	substitutions					705:717	single amino acid (aa) substitutions	682:717	single amino acid (aa) substitutions	682:717	Resultant mutations ranged from single amino acid (aa) substitutions to frameshift mutations causing premature stop codons, and led to specific differences in grain morphology, composition and (1,3;1,4)-β-glucan content.
32890421	5	43	theme	similar	972:978	arg1	content					999:1005	similar (1,3;1,4)-β-glucan content	972:1005	similar (1,3;1,4)-β-glucan content	972:1005	(1,3;1,4)-β-Glucan was absent in the grain of cslf6 knockout lines, whereas cslf9 knockout lines had similar (1,3;1,4)-β-glucan content to wild-type (WT).
32890421	9	44	dep	1,3	1544:1546	arg1	1,4					1548:1550	1,4	1548:1550	1,4	1548:1550	Our data indicate that multiple members of the CslF/H family fulfil important functions during grain development but, with the exception of HvCslF6, do not impact the abundance of (1,3;1,4)-β-glucan in mature grain.
32890421	6	45	from	changes	1056:1062	arg1	abundance					1071:1079	the abundance	1067:1079	the abundance of other cell-wall-related monosaccharides	1067:1122	However, cslf9 mutants showed changes in the abundance of other cell-wall-related monosaccharides compared with WT.
32890421	7	46	theme	lesser	1250:1255	arg1	extent					1257:1262	a lesser extent	1248:1262	a lesser extent	1248:1262	Thousand grain weight (TGW), grain length, width and surface area were altered in cslf6 knockouts, and to a lesser extent TGW in cslf9 knockouts.
32890421	5	47	dep	wild-type	1010:1018	arg1	WT					1021:1022	WT	1021:1022	WT	1021:1022	(1,3;1,4)-β-Glucan was absent in the grain of cslf6 knockout lines, whereas cslf9 knockout lines had similar (1,3;1,4)-β-glucan content to wild-type (WT).
32890421	4	48	theme	single	682:687	arg1	substitutions					705:717	single amino acid (aa) substitutions	682:717	single amino acid (aa) substitutions	682:717	Resultant mutations ranged from single amino acid (aa) substitutions to frameshift mutations causing premature stop codons, and led to specific differences in grain morphology, composition and (1,3;1,4)-β-glucan content.
32890421	3	49	dep	known	557:561	arg1	HvCslH1					576:582	HvCslH1	576:582	HvCslH1	576:582	We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases.
32890421	3	49	dep	known	557:561	arg1	HvCslF6					564:570	HvCslF6	564:570	HvCslF6	564:570	We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases.
32890421	4	50	dep	content	862:868	arg1	1,3					844:846	1,3	844:846	1,3;1,4	844:850	Resultant mutations ranged from single amino acid (aa) substitutions to frameshift mutations causing premature stop codons, and led to specific differences in grain morphology, composition and (1,3;1,4)-β-glucan content.
32890421	3	51	theme	reverse	413:419	arg1	approach					430:437	a reverse genetics approach	411:437	a reverse genetics approach	411:437	We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases.
32890421	5	52	theme	-β-glucan	989:997	arg1	content					999:1005	similar (1,3;1,4)-β-glucan content	972:1005	similar (1,3;1,4)-β-glucan content	972:1005	(1,3;1,4)-β-Glucan was absent in the grain of cslf6 knockout lines, whereas cslf9 knockout lines had similar (1,3;1,4)-β-glucan content to wild-type (WT).
32890421	9	53	from	abundance	1530:1538	arg1	grain					1572:1576	mature grain	1565:1576	mature grain	1565:1576	Our data indicate that multiple members of the CslF/H family fulfil important functions during grain development but, with the exception of HvCslF6, do not impact the abundance of (1,3;1,4)-β-glucan in mature grain.
32890421	3	54	theme	putative	589:596	arg1	synthases					639:647	known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases	557:647	known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases	557:647	We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases.
32890421	3	54	theme	putative	589:596	arg1	mutations					470:478	mutations	470:478	mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases	470:647	We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases.
32890421	7	55	theme	cslf6	1224:1228	arg1	knockouts					1230:1238	cslf6 knockouts	1224:1238	cslf6 knockouts	1224:1238	Thousand grain weight (TGW), grain length, width and surface area were altered in cslf6 knockouts, and to a lesser extent TGW in cslf9 knockouts.
32890421	4	56	theme	premature	751:759	arg1	codons					766:771	premature stop codons	751:771	premature stop codons	751:771	Resultant mutations ranged from single amino acid (aa) substitutions to frameshift mutations causing premature stop codons, and led to specific differences in grain morphology, composition and (1,3;1,4)-β-glucan content.
32890421	7	57	theme	surface	1195:1201	arg1	area					1203:1206	surface area	1195:1206	surface area	1195:1206	Thousand grain weight (TGW), grain length, width and surface area were altered in cslf6 knockouts, and to a lesser extent TGW in cslf9 knockouts.
32890421	1	58	theme	fibre	255:259	arg1	source					225:230	a source	223:230	a source of fermentable dietary fibre that protects against various human health conditions	223:313	Barley (Hordeum vulgare L) grain is comparatively rich in (1,3;1,4)-β-glucan, a source of fermentable dietary fibre that protects against various human health conditions.
32890421	1	58	theme	fibre	255:259	arg1	-β-glucan					212:220	-β-glucan	212:220	-β-glucan	212:220	Barley (Hordeum vulgare L) grain is comparatively rich in (1,3;1,4)-β-glucan, a source of fermentable dietary fibre that protects against various human health conditions.
32890421	9	59	theme	family	1417:1422	arg1	members					1395:1401	multiple members	1386:1401	multiple members of the CslF/H family	1386:1422	Our data indicate that multiple members of the CslF/H family fulfil important functions during grain development but, with the exception of HvCslF6, do not impact the abundance of (1,3;1,4)-β-glucan in mature grain.
32890421	0	60	theme	wall	116:119	arg1	content					136:142	the storage and cell wall polysaccharide content	95:142	content	136:142	Targeted mutation of barley (1,3;1,4)-β-glucan synthases reveals complex relationships between the storage and cell wall polysaccharide content.
32890421	4	61	from	differences	794:804	arg1	composition					827:837	composition	827:837	composition	827:837	Resultant mutations ranged from single amino acid (aa) substitutions to frameshift mutations causing premature stop codons, and led to specific differences in grain morphology, composition and (1,3;1,4)-β-glucan content.
32890421	4	61	from	differences	794:804	arg1	content					862:868	(1,3;1,4)-β-glucan content	843:868	(1,3;1,4)-β-glucan content	843:868	Resultant mutations ranged from single amino acid (aa) substitutions to frameshift mutations causing premature stop codons, and led to specific differences in grain morphology, composition and (1,3;1,4)-β-glucan content.
32890421	4	61	from	differences	794:804	arg1	morphology					815:824	grain morphology	809:824	grain morphology	809:824	Resultant mutations ranged from single amino acid (aa) substitutions to frameshift mutations causing premature stop codons, and led to specific differences in grain morphology, composition and (1,3;1,4)-β-glucan content.
32890421	3	62	theme	synthase-like	508:520	arg1	superfamily					533:543	the Cellulose synthase-like (Csl) gene superfamily	494:543	the Cellulose synthase-like (Csl) gene superfamily	494:543	We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases.
32890421	9	63	theme	important	1431:1439	arg1	functions					1441:1449	important functions	1431:1449	important functions	1431:1449	Our data indicate that multiple members of the CslF/H family fulfil important functions during grain development but, with the exception of HvCslF6, do not impact the abundance of (1,3;1,4)-β-glucan in mature grain.
32890421	6	64	theme	cell-wall-related	1090:1106	arg1	monosaccharides					1108:1122	other cell-wall-related monosaccharides	1084:1122	other cell-wall-related monosaccharides	1084:1122	However, cslf9 mutants showed changes in the abundance of other cell-wall-related monosaccharides compared with WT.
32890421	5	65	theme	knockout	953:960	arg1	lines					962:966	cslf9 knockout lines	947:966	cslf9 knockout lines	947:966	(1,3;1,4)-β-Glucan was absent in the grain of cslf6 knockout lines, whereas cslf9 knockout lines had similar (1,3;1,4)-β-glucan content to wild-type (WT).
32890421	9	66	theme	CslF/H	1410:1415	arg1	family					1417:1422	the CslF/H family	1406:1422	the CslF/H family	1406:1422	Our data indicate that multiple members of the CslF/H family fulfil important functions during grain development but, with the exception of HvCslF6, do not impact the abundance of (1,3;1,4)-β-glucan in mature grain.
32890421	3	67	from	mutations	470:478	arg1	members					483:489	members	483:489	members of the Cellulose synthase-like (Csl) gene superfamily	483:543	We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases.
32890421	1	68	from	rich	195:198	arg1	source					225:230	a source	223:230	a source of fermentable dietary fibre that protects against various human health conditions	223:313	Barley (Hordeum vulgare L) grain is comparatively rich in (1,3;1,4)-β-glucan, a source of fermentable dietary fibre that protects against various human health conditions.
32890421	1	68	from	rich	195:198	arg1	-β-glucan					212:220	-β-glucan	212:220	-β-glucan	212:220	Barley (Hordeum vulgare L) grain is comparatively rich in (1,3;1,4)-β-glucan, a source of fermentable dietary fibre that protects against various human health conditions.
32890421	3	69	theme	gene	528:531	arg1	superfamily					533:543	the Cellulose synthase-like (Csl) gene superfamily	494:543	the Cellulose synthase-like (Csl) gene superfamily	494:543	We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases.
32890421	0	70	theme	-β-glucan	37:45	arg1	synthases					47:55	barley (1,3;1,4)-β-glucan synthases	21:55	barley (1,3;1,4)-β-glucan synthases	21:55	Targeted mutation of barley (1,3;1,4)-β-glucan synthases reveals complex relationships between the storage and cell wall polysaccharide content.
32890421	3	71	theme	Csl	523:525	arg1	superfamily					533:543	the Cellulose synthase-like (Csl) gene superfamily	494:543	the Cellulose synthase-like (Csl) gene superfamily	494:543	We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases.
32890421	7	72	theme	Thousand	1142:1149	arg1	TGW					1165:1167	TGW	1165:1167	TGW	1165:1167	Thousand grain weight (TGW), grain length, width and surface area were altered in cslf6 knockouts, and to a lesser extent TGW in cslf9 knockouts.
32890421	7	72	theme	Thousand	1142:1149	arg1	weight					1157:1162	Thousand grain weight	1142:1162	Thousand grain weight (TGW)	1142:1168	Thousand grain weight (TGW), grain length, width and surface area were altered in cslf6 knockouts, and to a lesser extent TGW in cslf9 knockouts.
32890421	5	73	from	grain	908:912	arg1	absent					894:899	absent	894:899	absent	894:899	(1,3;1,4)-β-Glucan was absent in the grain of cslf6 knockout lines, whereas cslf9 knockout lines had similar (1,3;1,4)-β-glucan content to wild-type (WT).
32890421	5	74	dep	1,3	981:983	arg1	1,4					985:987	1,4	985:987	1,4	985:987	(1,3;1,4)-β-Glucan was absent in the grain of cslf6 knockout lines, whereas cslf9 knockout lines had similar (1,3;1,4)-β-glucan content to wild-type (WT).
32890421	1	75	theme	human	291:295	arg1	conditions					304:313	various human health conditions	283:313	various human health conditions	283:313	Barley (Hordeum vulgare L) grain is comparatively rich in (1,3;1,4)-β-glucan, a source of fermentable dietary fibre that protects against various human health conditions.
32890421	9	76	dep	-β-glucan	1552:1560	arg1	1,3					1544:1546	1,3	1544:1546	1,3;1,4	1544:1550	Our data indicate that multiple members of the CslF/H family fulfil important functions during grain development but, with the exception of HvCslF6, do not impact the abundance of (1,3;1,4)-β-glucan in mature grain.
32890421	4	77	theme	grain	809:813	arg1	morphology					815:824	grain morphology	809:824	grain morphology	809:824	Resultant mutations ranged from single amino acid (aa) substitutions to frameshift mutations causing premature stop codons, and led to specific differences in grain morphology, composition and (1,3;1,4)-β-glucan content.
32890421	5	78	dep	similar	972:978	arg1	1,3					981:983	1,3	981:983	1,3;1,4	981:987	(1,3;1,4)-β-Glucan was absent in the grain of cslf6 knockout lines, whereas cslf9 knockout lines had similar (1,3;1,4)-β-glucan content to wild-type (WT).
32890421	5	79	from	absent	894:899	arg1	grain					908:912	the grain	904:912	the grain of cslf6 knockout lines	904:936	(1,3;1,4)-β-Glucan was absent in the grain of cslf6 knockout lines, whereas cslf9 knockout lines had similar (1,3;1,4)-β-glucan content to wild-type (WT).
32890421	5	80	theme	cslf6	917:921	arg1	lines					932:936	cslf6 knockout lines	917:936	cslf6 knockout lines	917:936	(1,3;1,4)-β-Glucan was absent in the grain of cslf6 knockout lines, whereas cslf9 knockout lines had similar (1,3;1,4)-β-glucan content to wild-type (WT).
32890421	8	81	theme	cslf3	1288:1292	arg1	mutants					1304:1310	cslf3 and cslh1 mutants	1288:1310	cslf3 and cslh1 mutants	1288:1310	cslf3 and cslh1 mutants had no effect on grain (1,3;1,4)-β-glucan content.
32890421	2	82	theme	grain	329:333	arg1	content					354:360	low grain (1,3;1,4)-β-glucan content	325:360	low grain (1,3;1,4)-β-glucan content	325:360	However, low grain (1,3;1,4)-β-glucan content is preferred for brewing and distilling.
32890421	5	83	theme	lines	932:936	arg1	grain					908:912	the grain	904:912	the grain of cslf6 knockout lines	904:936	(1,3;1,4)-β-Glucan was absent in the grain of cslf6 knockout lines, whereas cslf9 knockout lines had similar (1,3;1,4)-β-glucan content to wild-type (WT).
32890421	8	84	theme	cslh1	1298:1302	arg1	mutants					1304:1310	cslf3 and cslh1 mutants	1288:1310	cslf3 and cslh1 mutants	1288:1310	cslf3 and cslh1 mutants had no effect on grain (1,3;1,4)-β-glucan content.
32890421	1	85	dep	Barley	145:150	arg1	vulgare					161:167	Hordeum vulgare	153:167	Hordeum vulgare L	153:169	Barley (Hordeum vulgare L) grain is comparatively rich in (1,3;1,4)-β-glucan, a source of fermentable dietary fibre that protects against various human health conditions.
32890421	8	86	theme	-β-glucan	1344:1352	arg1	content					1354:1360	grain (1,3;1,4)-β-glucan content	1329:1360	grain (1,3;1,4)-β-glucan content	1329:1360	cslf3 and cslh1 mutants had no effect on grain (1,3;1,4)-β-glucan content.
32890421	0	87	theme	barley	21:26	arg1	synthases					47:55	barley (1,3;1,4)-β-glucan synthases	21:55	barley (1,3;1,4)-β-glucan synthases	21:55	Targeted mutation of barley (1,3;1,4)-β-glucan synthases reveals complex relationships between the storage and cell wall polysaccharide content.
32890421	3	88	dep	putative	589:596	arg1	HvCslF9					611:617	HvCslF9	611:617	HvCslF9	611:617	We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases.
32890421	3	88	dep	putative	589:596	arg1	1,3					621:623	1,3	621:623	1,3;1,4	621:627	We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases.
32890421	3	88	dep	putative	589:596	arg1	HvCslF3					599:605	HvCslF3	599:605	HvCslF3	599:605	We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases.
32890421	2	89	theme	-β-glucan	344:352	arg1	content					354:360	low grain (1,3;1,4)-β-glucan content	325:360	low grain (1,3;1,4)-β-glucan content	325:360	However, low grain (1,3;1,4)-β-glucan content is preferred for brewing and distilling.
32890421	3	90	theme	known	557:561	arg1	synthases					639:647	known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases	557:647	known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases	557:647	We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases.
32890421	3	90	theme	known	557:561	arg1	mutations					470:478	mutations	470:478	mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases	470:647	We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode known (HvCslF6 and HvCslH1) and putative (HvCslF3 and HvCslF9) (1,3;1,4)-β-glucan synthases.
32890421	2	91	theme	1,4	340:342	arg1	content					354:360	low grain (1,3;1,4)-β-glucan content	325:360	low grain (1,3;1,4)-β-glucan content	325:360	However, low grain (1,3;1,4)-β-glucan content is preferred for brewing and distilling.
32890421	4	92	theme	amino	689:693	arg1	substitutions					705:717	single amino acid (aa) substitutions	682:717	single amino acid (aa) substitutions	682:717	Resultant mutations ranged from single amino acid (aa) substitutions to frameshift mutations causing premature stop codons, and led to specific differences in grain morphology, composition and (1,3;1,4)-β-glucan content.
32890421	2	93	theme	1,3	336:338	arg1	content					354:360	low grain (1,3;1,4)-β-glucan content	325:360	low grain (1,3;1,4)-β-glucan content	325:360	However, low grain (1,3;1,4)-β-glucan content is preferred for brewing and distilling.
32890421	0	94	dep	1,3	29:31	arg1	1,4					33:35	1,4	33:35	1,4	33:35	Targeted mutation of barley (1,3;1,4)-β-glucan synthases reveals complex relationships between the storage and cell wall polysaccharide content.
32890421	8	95	dep	1,3	1336:1338	arg1	1,4					1340:1342	1,4	1340:1342	1,4	1340:1342	cslf3 and cslh1 mutants had no effect on grain (1,3;1,4)-β-glucan content.
32890421	4	96	theme	-β-glucan	852:860	arg1	content					862:868	(1,3;1,4)-β-glucan content	843:868	(1,3;1,4)-β-glucan content	843:868	Resultant mutations ranged from single amino acid (aa) substitutions to frameshift mutations causing premature stop codons, and led to specific differences in grain morphology, composition and (1,3;1,4)-β-glucan content.
32890421	8	97	theme	grain	1329:1333	arg1	content					1354:1360	grain (1,3;1,4)-β-glucan content	1329:1360	grain (1,3;1,4)-β-glucan content	1329:1360	cslf3 and cslh1 mutants had no effect on grain (1,3;1,4)-β-glucan content.
32890421	0	98	dep	barley	21:26	arg1	1,3					29:31	1,3	29:31	1,3;1,4	29:35	Targeted mutation of barley (1,3;1,4)-β-glucan synthases reveals complex relationships between the storage and cell wall polysaccharide content.
32890421	6	99	theme	monosaccharides	1108:1122	arg1	abundance					1071:1079	the abundance	1067:1079	the abundance of other cell-wall-related monosaccharides	1067:1122	However, cslf9 mutants showed changes in the abundance of other cell-wall-related monosaccharides compared with WT.
32890421	9	100	theme	-β-glucan	1552:1560	arg1	abundance					1530:1538	the abundance	1526:1538	the abundance of (1,3;1,4)-β-glucan in mature grain	1526:1576	Our data indicate that multiple members of the CslF/H family fulfil important functions during grain development but, with the exception of HvCslF6, do not impact the abundance of (1,3;1,4)-β-glucan in mature grain.
32890421	5	101	dep	absent	894:899	arg1	whereas					939:945	whereas	939:945	whereas	939:945	(1,3;1,4)-β-Glucan was absent in the grain of cslf6 knockout lines, whereas cslf9 knockout lines had similar (1,3;1,4)-β-glucan content to wild-type (WT).
34693955	6	0	theme	chemical	1180:1187	arg1	conjugation					1189:1199	chemical conjugation	1180:1199	chemical conjugation with a Cy5.5 NHS ester	1180:1222	The main findings were: (1) real-time and noninvasive degradation monitoring of the ECMB composites until complete degradation could be realized by chemical conjugation with a Cy5.5 NHS ester.
34693955	1	1	theme	wall	167:170	arg1	defects					172:178	abdominal wall defects	157:178	abdominal wall defects	157:178	The repair of abdominal wall defects is currently a clinical challenge.
34693955	2	2	from	attention	321:329	arg1	repair					356:361	abdominal wall defect repair	334:361	abdominal wall defect repair	334:361	A naturally derived extracellular matrix (ECM) such as small intestine submucosa (SIS) has received great attention in abdominal wall defect repair because of its remarkable bioactivity, biodegradability and tissue regeneration.
34693955	7	3	theme	ECMB	1397:1400	arg1	composites					1402:1411	the ECMB composites	1393:1411	the ECMB composites	1393:1411	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	6	4	dep	realized	1168:1175	arg1	1					1057:1057	1	1057:1057	1	1057:1057	The main findings were: (1) real-time and noninvasive degradation monitoring of the ECMB composites until complete degradation could be realized by chemical conjugation with a Cy5.5 NHS ester.
34693955	5	5	theme	defect	869:874	arg1	repair					876:881	a full-thickness abdominal wall defect repair	837:881	a full-thickness abdominal wall defect repair	837:881	The tissue remodeling in the ECMB composites for a full-thickness abdominal wall defect repair was systematically investigated by a series of tests including wall thickness measurement, muscle regeneration analysis and angiogenesis assessment.
34693955	4	6	theme	fluorescent	611:621	arg1	dye					623:625	a near-infrared (NIR) fluorescent dye	589:625	a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester	589:641	In this study, a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester was used to label ECM-based (ECMB) composites consisting of SIS and chitosan/elastin electrospun nanofibers for monitoring material degradation.
34693955	0	7	theme	abdominal	114:122	arg1	model					136:140	a full-thickness abdominal wall defect model	97:140	a full-thickness abdominal wall defect model	97:140	Exploring the match between the degradation of the ECM-based composites and tissue remodeling in a full-thickness abdominal wall defect model.
34693955	3	8	theme	repair	552:557	arg1	effectiveness					559:571	ideal repair effectiveness	546:571	ideal repair effectiveness	546:571	The match between material degradation and tissue remodeling is very important for the realization of ideal repair effectiveness.
34693955	6	9	with	conjugation	1189:1199	arg1	ester					1218:1222	a Cy5.5 NHS ester	1206:1222	a Cy5.5 NHS ester	1206:1222	The main findings were: (1) real-time and noninvasive degradation monitoring of the ECMB composites until complete degradation could be realized by chemical conjugation with a Cy5.5 NHS ester.
34693955	7	10	theme	remodeling	1368:1377	arg1	efficiency					1379:1388	the tissue remodeling efficiency	1357:1388	the tissue remodeling efficiency in the ECMB composites	1357:1411	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	4	11	theme	Cy5.5	627:631	arg1	ester					637:641	a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester	589:641	a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester	589:641	In this study, a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester was used to label ECM-based (ECMB) composites consisting of SIS and chitosan/elastin electrospun nanofibers for monitoring material degradation.
34693955	0	12	theme	defect	129:134	arg1	model					136:140	a full-thickness abdominal wall defect model	97:140	a full-thickness abdominal wall defect model	97:140	Exploring the match between the degradation of the ECM-based composites and tissue remodeling in a full-thickness abdominal wall defect model.
34693955	6	13	theme	main	1036:1039	arg1	findings					1041:1048	The main findings	1032:1048	The main findings	1032:1048	The main findings were: (1) real-time and noninvasive degradation monitoring of the ECMB composites until complete degradation could be realized by chemical conjugation with a Cy5.5 NHS ester.
34693955	6	14	theme	Cy5.5	1208:1212	arg1	ester					1218:1222	a Cy5.5 NHS ester	1206:1222	a Cy5.5 NHS ester	1206:1222	The main findings were: (1) real-time and noninvasive degradation monitoring of the ECMB composites until complete degradation could be realized by chemical conjugation with a Cy5.5 NHS ester.
34693955	2	15	link	derived	227:233	arg1	matrix					249:254	A naturally derived extracellular matrix	215:254	A naturally derived extracellular matrix (ECM) such as small intestine submucosa (SIS)	215:300	A naturally derived extracellular matrix (ECM) such as small intestine submucosa (SIS) has received great attention in abdominal wall defect repair because of its remarkable bioactivity, biodegradability and tissue regeneration.
34693955	2	15	link	derived	227:233	arg1	ECM					257:259	ECM	257:259	ECM	257:259	A naturally derived extracellular matrix (ECM) such as small intestine submucosa (SIS) has received great attention in abdominal wall defect repair because of its remarkable bioactivity, biodegradability and tissue regeneration.
34693955	0	16	theme	ECM-based	51:59	arg1	composites					61:70	the ECM-based composites	47:70	the ECM-based composites	47:70	Exploring the match between the degradation of the ECM-based composites and tissue remodeling in a full-thickness abdominal wall defect model.
34693955	2	17	theme	extracellular	235:247	arg1	matrix					249:254	A naturally derived extracellular matrix	215:254	A naturally derived extracellular matrix (ECM) such as small intestine submucosa (SIS)	215:300	A naturally derived extracellular matrix (ECM) such as small intestine submucosa (SIS) has received great attention in abdominal wall defect repair because of its remarkable bioactivity, biodegradability and tissue regeneration.
34693955	2	17	theme	extracellular	235:247	arg1	ECM					257:259	ECM	257:259	ECM	257:259	A naturally derived extracellular matrix (ECM) such as small intestine submucosa (SIS) has received great attention in abdominal wall defect repair because of its remarkable bioactivity, biodegradability and tissue regeneration.
34693955	7	18	used	used	1309:1312	arg2	indicator					1332:1340	an intuitional indicator	1317:1340	an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites	1317:1411	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	7	18	used	used	1309:1312	arg2	thickness					1290:1298	the explant thickness	1278:1298	the explant thickness	1278:1298	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	5	19	theme	regeneration	981:992	arg1	analysis					994:1001	muscle regeneration analysis	974:1001	muscle regeneration analysis	974:1001	The tissue remodeling in the ECMB composites for a full-thickness abdominal wall defect repair was systematically investigated by a series of tests including wall thickness measurement, muscle regeneration analysis and angiogenesis assessment.
34693955	7	20	theme	intuitional	1320:1330	arg1	thickness					1290:1298	the explant thickness	1278:1298	the explant thickness	1278:1298	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	7	20	theme	intuitional	1320:1330	arg1	indicator					1332:1340	an intuitional indicator	1317:1340	an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites	1317:1411	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	4	21	theme	chitosan/elastin	711:726	arg1	nanofibers					740:749	chitosan/elastin electrospun nanofibers	711:749	chitosan/elastin electrospun nanofibers	711:749	In this study, a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester was used to label ECM-based (ECMB) composites consisting of SIS and chitosan/elastin electrospun nanofibers for monitoring material degradation.
34693955	5	22	theme	thickness	951:959	arg1	measurement					961:971	wall thickness measurement	946:971	wall thickness measurement	946:971	The tissue remodeling in the ECMB composites for a full-thickness abdominal wall defect repair was systematically investigated by a series of tests including wall thickness measurement, muscle regeneration analysis and angiogenesis assessment.
34693955	2	23	theme	remarkable	378:387	arg1	bioactivity					389:399	its remarkable bioactivity	374:399	its remarkable bioactivity	374:399	A naturally derived extracellular matrix (ECM) such as small intestine submucosa (SIS) has received great attention in abdominal wall defect repair because of its remarkable bioactivity, biodegradability and tissue regeneration.
34693955	5	24	theme	full-thickness	839:852	arg1	defect					869:874	a full-thickness abdominal wall defect	837:874	a full-thickness abdominal wall defect repair	837:881	The tissue remodeling in the ECMB composites for a full-thickness abdominal wall defect repair was systematically investigated by a series of tests including wall thickness measurement, muscle regeneration analysis and angiogenesis assessment.
34693955	7	25	dep	used	1309:1312	arg1	2					1226:1226	2	1226:1226	2	1226:1226	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	7	26	theme	explant	1282:1288	arg1	thickness					1290:1298	the explant thickness	1278:1298	the explant thickness	1278:1298	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	7	26	theme	explant	1282:1288	arg1	indicator					1332:1340	an intuitional indicator	1317:1340	an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites	1317:1411	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	2	27	theme	great	315:319	arg1	attention					321:329	great attention	315:329	great attention in abdominal wall defect repair	315:361	A naturally derived extracellular matrix (ECM) such as small intestine submucosa (SIS) has received great attention in abdominal wall defect repair because of its remarkable bioactivity, biodegradability and tissue regeneration.
34693955	3	28	theme	material	462:469	arg1	degradation					471:481	material degradation	462:481	material degradation	462:481	The match between material degradation and tissue remodeling is very important for the realization of ideal repair effectiveness.
34693955	8	29	theme	ECMB	1651:1654	arg1	composites					1656:1665	the ECMB composites	1647:1665	the ECMB composites	1647:1665	The present study could provide new insight into evaluating tissue repair effectiveness of the ECMB composites.
34693955	3	30	dep	The	444:446	arg1	match					448:452	match	448:452	match	448:452	The match between material degradation and tissue remodeling is very important for the realization of ideal repair effectiveness.
34693955	7	31	theme	defect	1264:1269	arg1	model					1271:1275	a full-thickness abdominal wall defect model	1232:1275	a full-thickness abdominal wall defect model	1232:1275	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	2	32	theme	wall	344:347	arg1	repair					356:361	abdominal wall defect repair	334:361	abdominal wall defect repair	334:361	A naturally derived extracellular matrix (ECM) such as small intestine submucosa (SIS) has received great attention in abdominal wall defect repair because of its remarkable bioactivity, biodegradability and tissue regeneration.
34693955	5	33	theme	abdominal	854:862	arg1	defect					869:874	a full-thickness abdominal wall defect	837:874	a full-thickness abdominal wall defect repair	837:881	The tissue remodeling in the ECMB composites for a full-thickness abdominal wall defect repair was systematically investigated by a series of tests including wall thickness measurement, muscle regeneration analysis and angiogenesis assessment.
34693955	7	34	theme	various	1465:1471	arg1	deposition					1505:1514	collagen deposition	1496:1514	collagen deposition	1496:1514	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	7	34	theme	various	1465:1471	arg1	regeneration					1542:1553	muscle regeneration	1535:1553	muscle regeneration	1535:1553	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	7	34	theme	various	1465:1471	arg1	angiogenesis					1517:1528	angiogenesis	1517:1528	angiogenesis	1517:1528	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	7	34	theme	various	1465:1471	arg1	examinations					1473:1484	various examinations	1465:1484	various examinations including collagen deposition, angiogenesis, and muscle regeneration	1465:1553	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	7	35	theme	abdominal	1249:1257	arg1	model					1271:1275	a full-thickness abdominal wall defect model	1232:1275	a full-thickness abdominal wall defect model	1232:1275	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	6	36	theme	degradation	1086:1096	arg1	monitoring					1098:1107	real-time and noninvasive degradation monitoring	1060:1107	real-time and noninvasive degradation monitoring of the ECMB composites until complete degradation	1060:1157	The main findings were: (1) real-time and noninvasive degradation monitoring of the ECMB composites until complete degradation could be realized by chemical conjugation with a Cy5.5 NHS ester.
34693955	8	37	theme	repair	1623:1628	arg1	effectiveness					1630:1642	tissue repair effectiveness	1616:1642	tissue repair effectiveness of the ECMB composites	1616:1665	The present study could provide new insight into evaluating tissue repair effectiveness of the ECMB composites.
34693955	2	38	theme	small	270:274	arg1	intestine					276:284	small intestine	270:284	small intestine submucosa (SIS)	270:300	A naturally derived extracellular matrix (ECM) such as small intestine submucosa (SIS) has received great attention in abdominal wall defect repair because of its remarkable bioactivity, biodegradability and tissue regeneration.
34693955	7	39	theme	indicator	1439:1447	arg1	accuracy					1422:1429	the accuracy	1418:1429	the accuracy of this indicator	1418:1447	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	1	40	theme	defects	172:178	arg1	repair					147:152	The repair	143:152	The repair of abdominal wall defects	143:178	The repair of abdominal wall defects is currently a clinical challenge.
34693955	1	40	theme	defects	172:178	arg1	challenge					204:212	a clinical challenge	193:212	a clinical challenge	193:212	The repair of abdominal wall defects is currently a clinical challenge.
34693955	1	41	theme	abdominal	157:165	arg1	defects					172:178	abdominal wall defects	157:178	abdominal wall defects	157:178	The repair of abdominal wall defects is currently a clinical challenge.
34693955	4	42	theme	NHS	633:635	arg1	ester					637:641	a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester	589:641	a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester	589:641	In this study, a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester was used to label ECM-based (ECMB) composites consisting of SIS and chitosan/elastin electrospun nanofibers for monitoring material degradation.
34693955	3	43	theme	effectiveness	559:571	arg1	realization					531:541	the realization	527:541	the realization of ideal repair effectiveness	527:571	The match between material degradation and tissue remodeling is very important for the realization of ideal repair effectiveness.
34693955	5	44	theme	wall	864:867	arg1	defect					869:874	a full-thickness abdominal wall defect	837:874	a full-thickness abdominal wall defect repair	837:881	The tissue remodeling in the ECMB composites for a full-thickness abdominal wall defect repair was systematically investigated by a series of tests including wall thickness measurement, muscle regeneration analysis and angiogenesis assessment.
34693955	0	45	theme	wall	124:127	arg1	model					136:140	a full-thickness abdominal wall defect model	97:140	a full-thickness abdominal wall defect model	97:140	Exploring the match between the degradation of the ECM-based composites and tissue remodeling in a full-thickness abdominal wall defect model.
34693955	3	46	theme	ideal	546:550	arg1	effectiveness					559:571	ideal repair effectiveness	546:571	ideal repair effectiveness	546:571	The match between material degradation and tissue remodeling is very important for the realization of ideal repair effectiveness.
34693955	5	47	theme	ECMB	817:820	arg1	composites					822:831	the ECMB composites	813:831	the ECMB composites for a full-thickness abdominal wall defect repair	813:881	The tissue remodeling in the ECMB composites for a full-thickness abdominal wall defect repair was systematically investigated by a series of tests including wall thickness measurement, muscle regeneration analysis and angiogenesis assessment.
34693955	2	48	theme	derived	227:233	arg1	matrix					249:254	A naturally derived extracellular matrix	215:254	A naturally derived extracellular matrix (ECM) such as small intestine submucosa (SIS)	215:300	A naturally derived extracellular matrix (ECM) such as small intestine submucosa (SIS) has received great attention in abdominal wall defect repair because of its remarkable bioactivity, biodegradability and tissue regeneration.
34693955	2	48	theme	derived	227:233	arg1	ECM					257:259	ECM	257:259	ECM	257:259	A naturally derived extracellular matrix (ECM) such as small intestine submucosa (SIS) has received great attention in abdominal wall defect repair because of its remarkable bioactivity, biodegradability and tissue regeneration.
34693955	1	49	theme	clinical	195:202	arg1	repair					147:152	The repair	143:152	The repair of abdominal wall defects	143:178	The repair of abdominal wall defects is currently a clinical challenge.
34693955	1	49	theme	clinical	195:202	arg1	challenge					204:212	a clinical challenge	193:212	a clinical challenge	193:212	The repair of abdominal wall defects is currently a clinical challenge.
34693955	4	50	theme	dye	623:625	arg1	ester					637:641	a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester	589:641	a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester	589:641	In this study, a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester was used to label ECM-based (ECMB) composites consisting of SIS and chitosan/elastin electrospun nanofibers for monitoring material degradation.
34693955	4	51	used	used	647:650	arg2	ester					637:641	a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester	589:641	a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester	589:641	In this study, a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester was used to label ECM-based (ECMB) composites consisting of SIS and chitosan/elastin electrospun nanofibers for monitoring material degradation.
34693955	7	52	theme	tissue	1361:1366	arg1	remodeling					1368:1377	tissue remodeling	1361:1377	the tissue remodeling efficiency in the ECMB composites	1357:1411	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	4	53	theme	ECMB	672:675	arg1	composites					678:687	ECM-based (ECMB) composites	661:687	ECM-based (ECMB) composites consisting of SIS and chitosan/elastin electrospun nanofibers	661:749	In this study, a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester was used to label ECM-based (ECMB) composites consisting of SIS and chitosan/elastin electrospun nanofibers for monitoring material degradation.
34693955	6	54	theme	NHS	1214:1216	arg1	ester					1218:1222	a Cy5.5 NHS ester	1206:1222	a Cy5.5 NHS ester	1206:1222	The main findings were: (1) real-time and noninvasive degradation monitoring of the ECMB composites until complete degradation could be realized by chemical conjugation with a Cy5.5 NHS ester.
34693955	6	55	theme	composites	1121:1130	arg1	monitoring					1098:1107	real-time and noninvasive degradation monitoring	1060:1107	real-time and noninvasive degradation monitoring of the ECMB composites until complete degradation	1060:1157	The main findings were: (1) real-time and noninvasive degradation monitoring of the ECMB composites until complete degradation could be realized by chemical conjugation with a Cy5.5 NHS ester.
34693955	0	56	theme	composites	61:70	arg1	degradation					32:42	the degradation	28:42	the degradation of the ECM-based composites	28:70	Exploring the match between the degradation of the ECM-based composites and tissue remodeling in a full-thickness abdominal wall defect model.
34693955	0	56	theme	composites	61:70	arg1	remodeling					83:92	tissue remodeling	76:92	tissue remodeling	76:92	Exploring the match between the degradation of the ECM-based composites and tissue remodeling in a full-thickness abdominal wall defect model.
34693955	0	57	theme	tissue	76:81	arg1	remodeling					83:92	tissue remodeling	76:92	tissue remodeling	76:92	Exploring the match between the degradation of the ECM-based composites and tissue remodeling in a full-thickness abdominal wall defect model.
34693955	4	58	theme	ECM-based	661:669	arg1	composites					678:687	ECM-based (ECMB) composites	661:687	ECM-based (ECMB) composites consisting of SIS and chitosan/elastin electrospun nanofibers	661:749	In this study, a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester was used to label ECM-based (ECMB) composites consisting of SIS and chitosan/elastin electrospun nanofibers for monitoring material degradation.
34693955	2	59	theme	intestine	276:284	arg1	submucosa					286:294	small intestine submucosa	270:294	small intestine submucosa (SIS)	270:300	A naturally derived extracellular matrix (ECM) such as small intestine submucosa (SIS) has received great attention in abdominal wall defect repair because of its remarkable bioactivity, biodegradability and tissue regeneration.
34693955	2	59	theme	intestine	276:284	arg1	SIS					297:299	SIS	297:299	SIS	297:299	A naturally derived extracellular matrix (ECM) such as small intestine submucosa (SIS) has received great attention in abdominal wall defect repair because of its remarkable bioactivity, biodegradability and tissue regeneration.
34693955	4	60	theme	electrospun	728:738	arg1	nanofibers					740:749	chitosan/elastin electrospun nanofibers	711:749	chitosan/elastin electrospun nanofibers	711:749	In this study, a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester was used to label ECM-based (ECMB) composites consisting of SIS and chitosan/elastin electrospun nanofibers for monitoring material degradation.
34693955	6	61	theme	ECMB	1116:1119	arg1	composites					1121:1130	the ECMB composites	1112:1130	the ECMB composites	1112:1130	The main findings were: (1) real-time and noninvasive degradation monitoring of the ECMB composites until complete degradation could be realized by chemical conjugation with a Cy5.5 NHS ester.
34693955	0	62	theme	full-thickness	99:112	arg1	model					136:140	a full-thickness abdominal wall defect model	97:140	a full-thickness abdominal wall defect model	97:140	Exploring the match between the degradation of the ECM-based composites and tissue remodeling in a full-thickness abdominal wall defect model.
34693955	5	63	theme	muscle	974:979	arg1	regeneration					981:992	muscle regeneration	974:992	muscle regeneration analysis	974:1001	The tissue remodeling in the ECMB composites for a full-thickness abdominal wall defect repair was systematically investigated by a series of tests including wall thickness measurement, muscle regeneration analysis and angiogenesis assessment.
34693955	6	64	theme	noninvasive	1074:1084	arg1	monitoring					1098:1107	real-time and noninvasive degradation monitoring	1060:1107	real-time and noninvasive degradation monitoring of the ECMB composites until complete degradation	1060:1157	The main findings were: (1) real-time and noninvasive degradation monitoring of the ECMB composites until complete degradation could be realized by chemical conjugation with a Cy5.5 NHS ester.
34693955	5	65	theme	tissue	792:797	arg1	remodeling					799:808	The tissue remodeling	788:808	The tissue remodeling in the ECMB composites for a full-thickness abdominal wall defect repair	788:881	The tissue remodeling in the ECMB composites for a full-thickness abdominal wall defect repair was systematically investigated by a series of tests including wall thickness measurement, muscle regeneration analysis and angiogenesis assessment.
34693955	5	66	from	remodeling	799:808	arg1	composites					822:831	the ECMB composites	813:831	the ECMB composites for a full-thickness abdominal wall defect repair	813:881	The tissue remodeling in the ECMB composites for a full-thickness abdominal wall defect repair was systematically investigated by a series of tests including wall thickness measurement, muscle regeneration analysis and angiogenesis assessment.
34693955	5	67	theme	tests	930:934	arg1	series					920:925	a series	918:925	a series of tests including wall thickness measurement, muscle regeneration analysis and angiogenesis assessment	918:1029	The tissue remodeling in the ECMB composites for a full-thickness abdominal wall defect repair was systematically investigated by a series of tests including wall thickness measurement, muscle regeneration analysis and angiogenesis assessment.
34693955	6	68	theme	real-time	1060:1068	arg1	monitoring					1098:1107	real-time and noninvasive degradation monitoring	1060:1107	real-time and noninvasive degradation monitoring of the ECMB composites until complete degradation	1060:1157	The main findings were: (1) real-time and noninvasive degradation monitoring of the ECMB composites until complete degradation could be realized by chemical conjugation with a Cy5.5 NHS ester.
34693955	2	69	theme	tissue	423:428	arg1	regeneration					430:441	tissue regeneration	423:441	tissue regeneration	423:441	A naturally derived extracellular matrix (ECM) such as small intestine submucosa (SIS) has received great attention in abdominal wall defect repair because of its remarkable bioactivity, biodegradability and tissue regeneration.
34693955	2	70	theme	defect	349:354	arg1	repair					356:361	abdominal wall defect repair	334:361	abdominal wall defect repair	334:361	A naturally derived extracellular matrix (ECM) such as small intestine submucosa (SIS) has received great attention in abdominal wall defect repair because of its remarkable bioactivity, biodegradability and tissue regeneration.
34693955	0	71	dep	the	10:12	arg1	match					14:18	match	14:18	match between the degradation of the ECM-based composites and tissue remodeling in a full-thickness abdominal wall defect model	14:140	Exploring the match between the degradation of the ECM-based composites and tissue remodeling in a full-thickness abdominal wall defect model.
34693955	5	72	theme	wall	946:949	arg1	measurement					961:971	wall thickness measurement	946:971	wall thickness measurement	946:971	The tissue remodeling in the ECMB composites for a full-thickness abdominal wall defect repair was systematically investigated by a series of tests including wall thickness measurement, muscle regeneration analysis and angiogenesis assessment.
34693955	8	73	theme	present	1560:1566	arg1	study					1568:1572	The present study	1556:1572	The present study	1556:1572	The present study could provide new insight into evaluating tissue repair effectiveness of the ECMB composites.
34693955	7	74	theme	muscle	1535:1540	arg1	regeneration					1542:1553	muscle regeneration	1535:1553	muscle regeneration	1535:1553	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	7	75	from	efficiency	1379:1388	arg1	composites					1402:1411	the ECMB composites	1393:1411	the ECMB composites	1393:1411	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	4	76	theme	NIR	606:608	arg1	dye					623:625	a near-infrared (NIR) fluorescent dye	589:625	a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester	589:641	In this study, a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester was used to label ECM-based (ECMB) composites consisting of SIS and chitosan/elastin electrospun nanofibers for monitoring material degradation.
34693955	6	77	theme	complete	1138:1145	arg1	degradation					1147:1157	complete degradation	1138:1157	complete degradation	1138:1157	The main findings were: (1) real-time and noninvasive degradation monitoring of the ECMB composites until complete degradation could be realized by chemical conjugation with a Cy5.5 NHS ester.
34693955	8	78	theme	new	1588:1590	arg1	insight					1592:1598	new insight	1588:1598	new insight into evaluating tissue repair effectiveness of the ECMB composites	1588:1665	The present study could provide new insight into evaluating tissue repair effectiveness of the ECMB composites.
34693955	3	79	theme	tissue	487:492	arg1	remodeling					494:503	tissue remodeling	487:503	tissue remodeling	487:503	The match between material degradation and tissue remodeling is very important for the realization of ideal repair effectiveness.
34693955	8	80	theme	composites	1656:1665	arg1	effectiveness					1630:1642	tissue repair effectiveness	1616:1642	tissue repair effectiveness of the ECMB composites	1616:1665	The present study could provide new insight into evaluating tissue repair effectiveness of the ECMB composites.
34693955	7	81	theme	wall	1259:1262	arg1	model					1271:1275	a full-thickness abdominal wall defect model	1232:1275	a full-thickness abdominal wall defect model	1232:1275	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	4	82	theme	near-infrared	591:603	arg1	dye					623:625	a near-infrared (NIR) fluorescent dye	589:625	a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester	589:641	In this study, a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester was used to label ECM-based (ECMB) composites consisting of SIS and chitosan/elastin electrospun nanofibers for monitoring material degradation.
34693955	4	83	theme	material	766:773	arg1	degradation					775:785	material degradation	766:785	material degradation	766:785	In this study, a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester was used to label ECM-based (ECMB) composites consisting of SIS and chitosan/elastin electrospun nanofibers for monitoring material degradation.
34693955	7	84	theme	collagen	1496:1503	arg1	deposition					1505:1514	collagen deposition	1496:1514	collagen deposition	1496:1514	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	5	85	theme	angiogenesis	1007:1018	arg1	assessment					1020:1029	angiogenesis assessment	1007:1029	angiogenesis assessment	1007:1029	The tissue remodeling in the ECMB composites for a full-thickness abdominal wall defect repair was systematically investigated by a series of tests including wall thickness measurement, muscle regeneration analysis and angiogenesis assessment.
34693955	7	86	theme	full-thickness	1234:1247	arg1	model					1271:1275	a full-thickness abdominal wall defect model	1232:1275	a full-thickness abdominal wall defect model	1232:1275	(2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration.
34693955	8	87	theme	tissue	1616:1621	arg1	effectiveness					1630:1642	tissue repair effectiveness	1616:1642	tissue repair effectiveness of the ECMB composites	1616:1665	The present study could provide new insight into evaluating tissue repair effectiveness of the ECMB composites.
34693955	2	88	theme	abdominal	334:342	arg1	repair					356:361	abdominal wall defect repair	334:361	abdominal wall defect repair	334:361	A naturally derived extracellular matrix (ECM) such as small intestine submucosa (SIS) has received great attention in abdominal wall defect repair because of its remarkable bioactivity, biodegradability and tissue regeneration.
34808780	5	0	with	skeleton	743:750	arg1	proportions					811:821	higher B-type starch proportions	790:821	higher B-type starch proportions	790:821	The gluten skeleton of the wheat lines SS 2 and ZZ 2 with higher B-type starch proportions exhibited fewer breakages along with the rise of dough temperature during mixing.
34808780	4	1	theme	dough	635:639	arg1	development					641:651	dough development	635:651	dough development	635:651	Compared to ZZ 1 and ZZ 2, both SS 1 and SS 2 carried superior HMW-GS alleles, and exhibited extended dough development and stability time, indicating their significant dough mixing characteristics.
34808780	5	2	theme	fewer	833:837	arg1	breakages					839:847	fewer breakages	833:847	fewer breakages	833:847	The gluten skeleton of the wheat lines SS 2 and ZZ 2 with higher B-type starch proportions exhibited fewer breakages along with the rise of dough temperature during mixing.
34808780	4	3	theme	extended	626:633	arg1	time					667:670	extended dough development and stability time	626:670	extended dough development and stability time	626:670	Compared to ZZ 1 and ZZ 2, both SS 1 and SS 2 carried superior HMW-GS alleles, and exhibited extended dough development and stability time, indicating their significant dough mixing characteristics.
34808780	5	4	dep	lines	765:769	arg1	ZZ					780:781	ZZ 2	780:783	ZZ 2	780:783	The gluten skeleton of the wheat lines SS 2 and ZZ 2 with higher B-type starch proportions exhibited fewer breakages along with the rise of dough temperature during mixing.
34808780	5	4	dep	lines	765:769	arg1	lines					765:769	the wheat lines SS 2 and ZZ 2	755:783	the wheat lines SS 2 and ZZ 2	755:783	The gluten skeleton of the wheat lines SS 2 and ZZ 2 with higher B-type starch proportions exhibited fewer breakages along with the rise of dough temperature during mixing.
34808780	5	4	dep	lines	765:769	arg1	SS					771:772	SS 2	771:774	SS 2	771:774	The gluten skeleton of the wheat lines SS 2 and ZZ 2 with higher B-type starch proportions exhibited fewer breakages along with the rise of dough temperature during mixing.
34808780	2	5	theme	glutenin	316:323	arg1	HMW-GS					335:340	HMW-GS	335:340	HMW-GS	335:340	However, the combined influence of high-molecular-weight glutenin subunits (HMW-GS) and starch on dough mixing and rheological properties requires elucidation.
34808780	2	5	theme	glutenin	316:323	arg1	subunits					325:332	high-molecular-weight glutenin subunits	294:332	high-molecular-weight glutenin subunits (HMW-GS)	294:341	However, the combined influence of high-molecular-weight glutenin subunits (HMW-GS) and starch on dough mixing and rheological properties requires elucidation.
34808780	6	6	theme	B-type	923:928	arg1	starch					930:935	B-type starch	923:935	B-type starch	923:935	Higher content of B-type starch strengthens interaction between starch and gluten skeleton at the dough heating stage, suggesting a specific range of B-type starch proportion can improve dough mixing characteristics.
34808780	6	7	theme	mixing	1098:1103	arg1	characteristics					1105:1119	dough mixing characteristics	1092:1119	dough mixing characteristics	1092:1119	Higher content of B-type starch strengthens interaction between starch and gluten skeleton at the dough heating stage, suggesting a specific range of B-type starch proportion can improve dough mixing characteristics.
34808780	6	8	theme	dough	1092:1096	arg1	characteristics					1105:1119	dough mixing characteristics	1092:1119	dough mixing characteristics	1092:1119	Higher content of B-type starch strengthens interaction between starch and gluten skeleton at the dough heating stage, suggesting a specific range of B-type starch proportion can improve dough mixing characteristics.
34808780	4	9	theme	dough	702:706	arg1	characteristics					715:729	their significant dough mixing characteristics	684:729	their significant dough mixing characteristics	684:729	Compared to ZZ 1 and ZZ 2, both SS 1 and SS 2 carried superior HMW-GS alleles, and exhibited extended dough development and stability time, indicating their significant dough mixing characteristics.
34808780	2	10	theme	high-molecular-weight	294:314	arg1	HMW-GS					335:340	HMW-GS	335:340	HMW-GS	335:340	However, the combined influence of high-molecular-weight glutenin subunits (HMW-GS) and starch on dough mixing and rheological properties requires elucidation.
34808780	2	10	theme	high-molecular-weight	294:314	arg1	subunits					325:332	high-molecular-weight glutenin subunits	294:332	high-molecular-weight glutenin subunits (HMW-GS)	294:341	However, the combined influence of high-molecular-weight glutenin subunits (HMW-GS) and starch on dough mixing and rheological properties requires elucidation.
34808780	6	11	theme	proportion	1069:1078	arg1	range					1046:1050	a specific range	1035:1050	a specific range of B-type starch proportion	1035:1078	Higher content of B-type starch strengthens interaction between starch and gluten skeleton at the dough heating stage, suggesting a specific range of B-type starch proportion can improve dough mixing characteristics.
34808780	6	12	theme	heating	1009:1015	arg1	stage					1017:1021	the dough heating stage	999:1021	the dough heating stage	999:1021	Higher content of B-type starch strengthens interaction between starch and gluten skeleton at the dough heating stage, suggesting a specific range of B-type starch proportion can improve dough mixing characteristics.
34808780	4	13	theme	HMW-GS	596:601	arg1	alleles					603:609	superior HMW-GS alleles	587:609	superior HMW-GS alleles	587:609	Compared to ZZ 1 and ZZ 2, both SS 1 and SS 2 carried superior HMW-GS alleles, and exhibited extended dough development and stability time, indicating their significant dough mixing characteristics.
34808780	0	14	theme	gluten	104:109	arg1	micro-structure					111:125	gluten micro-structure	104:125	gluten micro-structure	104:125	Interaction of B-type starch with gluten skeleton improves wheat dough mixing properties by stabilizing gluten micro-structure.
34808780	3	15	theme	inbred	441:446	arg1	ZZ					476:477	ZZ 2	476:479	ZZ 2	476:479	Thus four recombinant inbred lines, SS 1, SS 2, ZZ 1 and ZZ 2, were selected based on their HMW-GSs compositions.
34808780	3	15	theme	inbred	441:446	arg1	ZZ					467:468	ZZ 1	467:470	ZZ 1	467:470	Thus four recombinant inbred lines, SS 1, SS 2, ZZ 1 and ZZ 2, were selected based on their HMW-GSs compositions.
34808780	3	15	theme	inbred	441:446	arg1	SS					455:456	SS 1	455:458	SS 1	455:458	Thus four recombinant inbred lines, SS 1, SS 2, ZZ 1 and ZZ 2, were selected based on their HMW-GSs compositions.
34808780	3	15	theme	inbred	441:446	arg1	SS					461:462	SS 2	461:464	SS 2	461:464	Thus four recombinant inbred lines, SS 1, SS 2, ZZ 1 and ZZ 2, were selected based on their HMW-GSs compositions.
34808780	3	15	theme	inbred	441:446	arg1	lines					448:452	four recombinant inbred lines	424:452	four recombinant inbred lines	424:452	Thus four recombinant inbred lines, SS 1, SS 2, ZZ 1 and ZZ 2, were selected based on their HMW-GSs compositions.
34808780	1	16	theme	mixing	240:245	arg1	properties					247:256	dough mixing properties	234:256	dough mixing properties	234:256	Some recent studies have revealed individual and the combined interactions of gluten and starch affecting dough mixing properties.
34808780	1	17	theme	combined	181:188	arg1	interactions					190:201	the combined interactions	177:201	the combined interactions of gluten and starch affecting dough mixing properties	177:256	Some recent studies have revealed individual and the combined interactions of gluten and starch affecting dough mixing properties.
34808780	5	18	theme	temperature	878:888	arg1	rise					864:867	the rise	860:867	the rise of dough temperature during mixing	860:902	The gluten skeleton of the wheat lines SS 2 and ZZ 2 with higher B-type starch proportions exhibited fewer breakages along with the rise of dough temperature during mixing.
34808780	5	19	theme	B-type	797:802	arg1	proportions					811:821	higher B-type starch proportions	790:821	higher B-type starch proportions	790:821	The gluten skeleton of the wheat lines SS 2 and ZZ 2 with higher B-type starch proportions exhibited fewer breakages along with the rise of dough temperature during mixing.
34808780	5	20	theme	wheat	759:763	arg1	ZZ					780:781	ZZ 2	780:783	ZZ 2	780:783	The gluten skeleton of the wheat lines SS 2 and ZZ 2 with higher B-type starch proportions exhibited fewer breakages along with the rise of dough temperature during mixing.
34808780	5	20	theme	wheat	759:763	arg1	lines					765:769	the wheat lines SS 2 and ZZ 2	755:783	the wheat lines SS 2 and ZZ 2	755:783	The gluten skeleton of the wheat lines SS 2 and ZZ 2 with higher B-type starch proportions exhibited fewer breakages along with the rise of dough temperature during mixing.
34808780	5	20	theme	wheat	759:763	arg1	SS					771:772	SS 2	771:774	SS 2	771:774	The gluten skeleton of the wheat lines SS 2 and ZZ 2 with higher B-type starch proportions exhibited fewer breakages along with the rise of dough temperature during mixing.
34808780	5	21	theme	dough	872:876	arg1	temperature					878:888	dough temperature	872:888	dough temperature during mixing	872:902	The gluten skeleton of the wheat lines SS 2 and ZZ 2 with higher B-type starch proportions exhibited fewer breakages along with the rise of dough temperature during mixing.
34808780	4	22	theme	mixing	708:713	arg1	characteristics					715:729	their significant dough mixing characteristics	684:729	their significant dough mixing characteristics	684:729	Compared to ZZ 1 and ZZ 2, both SS 1 and SS 2 carried superior HMW-GS alleles, and exhibited extended dough development and stability time, indicating their significant dough mixing characteristics.
34808780	5	23	theme	starch	804:809	arg1	proportions					811:821	higher B-type starch proportions	790:821	higher B-type starch proportions	790:821	The gluten skeleton of the wheat lines SS 2 and ZZ 2 with higher B-type starch proportions exhibited fewer breakages along with the rise of dough temperature during mixing.
34808780	0	24	theme	starch	22:27	arg1	Interaction					0:10	Interaction	0:10	Interaction of B-type starch with gluten skeleton	0:48	Interaction of B-type starch with gluten skeleton improves wheat dough mixing properties by stabilizing gluten micro-structure.
34808780	6	25	theme	Higher	905:910	arg1	content					912:918	Higher content	905:918	Higher content of B-type starch	905:935	Higher content of B-type starch strengthens interaction between starch and gluten skeleton at the dough heating stage, suggesting a specific range of B-type starch proportion can improve dough mixing characteristics.
34808780	4	26	theme	significant	690:700	arg1	characteristics					715:729	their significant dough mixing characteristics	684:729	their significant dough mixing characteristics	684:729	Compared to ZZ 1 and ZZ 2, both SS 1 and SS 2 carried superior HMW-GS alleles, and exhibited extended dough development and stability time, indicating their significant dough mixing characteristics.
34808780	1	27	theme	gluten	206:211	arg1	interactions					190:201	the combined interactions	177:201	the combined interactions of gluten and starch affecting dough mixing properties	177:256	Some recent studies have revealed individual and the combined interactions of gluten and starch affecting dough mixing properties.
34808780	1	27	theme	gluten	206:211	arg1	individual					162:171	individual	162:171	individual	162:171	Some recent studies have revealed individual and the combined interactions of gluten and starch affecting dough mixing properties.
34808780	0	28	theme	B-type	15:20	arg1	starch					22:27	B-type starch	15:27	B-type starch	15:27	Interaction of B-type starch with gluten skeleton improves wheat dough mixing properties by stabilizing gluten micro-structure.
34808780	2	29	theme	subunits	325:332	arg1	influence					281:289	the combined influence	268:289	the combined influence of high-molecular-weight glutenin subunits (HMW-GS) and starch on dough mixing and rheological properties	268:395	However, the combined influence of high-molecular-weight glutenin subunits (HMW-GS) and starch on dough mixing and rheological properties requires elucidation.
34808780	5	30	theme	higher	790:795	arg1	proportions					811:821	higher B-type starch proportions	790:821	higher B-type starch proportions	790:821	The gluten skeleton of the wheat lines SS 2 and ZZ 2 with higher B-type starch proportions exhibited fewer breakages along with the rise of dough temperature during mixing.
34808780	0	31	theme	gluten	34:39	arg1	skeleton					41:48	gluten skeleton	34:48	gluten skeleton	34:48	Interaction of B-type starch with gluten skeleton improves wheat dough mixing properties by stabilizing gluten micro-structure.
34808780	6	32	theme	starch	1062:1067	arg1	proportion					1069:1078	B-type starch proportion	1055:1078	B-type starch proportion	1055:1078	Higher content of B-type starch strengthens interaction between starch and gluten skeleton at the dough heating stage, suggesting a specific range of B-type starch proportion can improve dough mixing characteristics.
34808780	4	33	contain	carried	579:585	arg2	alleles					603:609	superior HMW-GS alleles	587:609	superior HMW-GS alleles	587:609	Compared to ZZ 1 and ZZ 2, both SS 1 and SS 2 carried superior HMW-GS alleles, and exhibited extended dough development and stability time, indicating their significant dough mixing characteristics.
34808780	4	33	contain	carried	579:585	arg1	SS					565:566	SS 1	565:568	SS 1	565:568	Compared to ZZ 1 and ZZ 2, both SS 1 and SS 2 carried superior HMW-GS alleles, and exhibited extended dough development and stability time, indicating their significant dough mixing characteristics.
34808780	4	33	contain	carried	579:585	arg1	SS					574:575	SS 2	574:577	SS 2	574:577	Compared to ZZ 1 and ZZ 2, both SS 1 and SS 2 carried superior HMW-GS alleles, and exhibited extended dough development and stability time, indicating their significant dough mixing characteristics.
34808780	6	34	theme	dough	1003:1007	arg1	stage					1017:1021	the dough heating stage	999:1021	the dough heating stage	999:1021	Higher content of B-type starch strengthens interaction between starch and gluten skeleton at the dough heating stage, suggesting a specific range of B-type starch proportion can improve dough mixing characteristics.
34808780	1	35	theme	starch	217:222	arg1	interactions					190:201	the combined interactions	177:201	the combined interactions of gluten and starch affecting dough mixing properties	177:256	Some recent studies have revealed individual and the combined interactions of gluten and starch affecting dough mixing properties.
34808780	1	35	theme	starch	217:222	arg1	individual					162:171	individual	162:171	individual	162:171	Some recent studies have revealed individual and the combined interactions of gluten and starch affecting dough mixing properties.
34808780	5	36	theme	gluten	736:741	arg1	skeleton					743:750	The gluten skeleton	732:750	The gluten skeleton of the wheat lines SS 2 and ZZ 2 with higher B-type starch proportions	732:821	The gluten skeleton of the wheat lines SS 2 and ZZ 2 with higher B-type starch proportions exhibited fewer breakages along with the rise of dough temperature during mixing.
34808780	6	37	theme	starch	930:935	arg1	content					912:918	Higher content	905:918	Higher content of B-type starch	905:935	Higher content of B-type starch strengthens interaction between starch and gluten skeleton at the dough heating stage, suggesting a specific range of B-type starch proportion can improve dough mixing characteristics.
34808780	5	38	theme	lines	765:769	arg1	skeleton					743:750	The gluten skeleton	732:750	The gluten skeleton of the wheat lines SS 2 and ZZ 2 with higher B-type starch proportions	732:821	The gluten skeleton of the wheat lines SS 2 and ZZ 2 with higher B-type starch proportions exhibited fewer breakages along with the rise of dough temperature during mixing.
34808780	6	39	theme	gluten	980:985	arg1	skeleton					987:994	gluten skeleton	980:994	gluten skeleton	980:994	Higher content of B-type starch strengthens interaction between starch and gluten skeleton at the dough heating stage, suggesting a specific range of B-type starch proportion can improve dough mixing characteristics.
34808780	6	40	theme	B-type	1055:1060	arg1	proportion					1069:1078	B-type starch proportion	1055:1078	B-type starch proportion	1055:1078	Higher content of B-type starch strengthens interaction between starch and gluten skeleton at the dough heating stage, suggesting a specific range of B-type starch proportion can improve dough mixing characteristics.
34808780	2	41	theme	dough	357:361	arg1	mixing					363:368	dough mixing	357:368	dough mixing	357:368	However, the combined influence of high-molecular-weight glutenin subunits (HMW-GS) and starch on dough mixing and rheological properties requires elucidation.
34808780	3	42	theme	recombinant	429:439	arg1	ZZ					476:477	ZZ 2	476:479	ZZ 2	476:479	Thus four recombinant inbred lines, SS 1, SS 2, ZZ 1 and ZZ 2, were selected based on their HMW-GSs compositions.
34808780	3	42	theme	recombinant	429:439	arg1	ZZ					467:468	ZZ 1	467:470	ZZ 1	467:470	Thus four recombinant inbred lines, SS 1, SS 2, ZZ 1 and ZZ 2, were selected based on their HMW-GSs compositions.
34808780	3	42	theme	recombinant	429:439	arg1	SS					455:456	SS 1	455:458	SS 1	455:458	Thus four recombinant inbred lines, SS 1, SS 2, ZZ 1 and ZZ 2, were selected based on their HMW-GSs compositions.
34808780	3	42	theme	recombinant	429:439	arg1	SS					461:462	SS 2	461:464	SS 2	461:464	Thus four recombinant inbred lines, SS 1, SS 2, ZZ 1 and ZZ 2, were selected based on their HMW-GSs compositions.
34808780	3	42	theme	recombinant	429:439	arg1	lines					448:452	four recombinant inbred lines	424:452	four recombinant inbred lines	424:452	Thus four recombinant inbred lines, SS 1, SS 2, ZZ 1 and ZZ 2, were selected based on their HMW-GSs compositions.
34808780	4	43	theme	development	641:651	arg1	time					667:670	extended dough development and stability time	626:670	extended dough development and stability time	626:670	Compared to ZZ 1 and ZZ 2, both SS 1 and SS 2 carried superior HMW-GS alleles, and exhibited extended dough development and stability time, indicating their significant dough mixing characteristics.
34808780	2	44	theme	combined	272:279	arg1	influence					281:289	the combined influence	268:289	the combined influence of high-molecular-weight glutenin subunits (HMW-GS) and starch on dough mixing and rheological properties	268:395	However, the combined influence of high-molecular-weight glutenin subunits (HMW-GS) and starch on dough mixing and rheological properties requires elucidation.
34808780	0	45	with	Interaction	0:10	arg1	skeleton					41:48	gluten skeleton	34:48	gluten skeleton	34:48	Interaction of B-type starch with gluten skeleton improves wheat dough mixing properties by stabilizing gluten micro-structure.
34808780	1	46	theme	recent	133:138	arg1	studies					140:146	Some recent studies	128:146	Some recent studies	128:146	Some recent studies have revealed individual and the combined interactions of gluten and starch affecting dough mixing properties.
34808780	2	47	from	influence	281:289	arg1	mixing					363:368	dough mixing	357:368	dough mixing	357:368	However, the combined influence of high-molecular-weight glutenin subunits (HMW-GS) and starch on dough mixing and rheological properties requires elucidation.
34808780	2	47	from	influence	281:289	arg1	properties					386:395	rheological properties	374:395	rheological properties	374:395	However, the combined influence of high-molecular-weight glutenin subunits (HMW-GS) and starch on dough mixing and rheological properties requires elucidation.
34808780	1	48	theme	dough	234:238	arg1	properties					247:256	dough mixing properties	234:256	dough mixing properties	234:256	Some recent studies have revealed individual and the combined interactions of gluten and starch affecting dough mixing properties.
34808780	3	49	theme	HMW-GSs	511:517	arg1	compositions					519:530	their HMW-GSs compositions	505:530	their HMW-GSs compositions	505:530	Thus four recombinant inbred lines, SS 1, SS 2, ZZ 1 and ZZ 2, were selected based on their HMW-GSs compositions.
34808780	4	50	theme	stability	657:665	arg1	time					667:670	extended dough development and stability time	626:670	extended dough development and stability time	626:670	Compared to ZZ 1 and ZZ 2, both SS 1 and SS 2 carried superior HMW-GS alleles, and exhibited extended dough development and stability time, indicating their significant dough mixing characteristics.
34808780	2	51	theme	starch	347:352	arg1	influence					281:289	the combined influence	268:289	the combined influence of high-molecular-weight glutenin subunits (HMW-GS) and starch on dough mixing and rheological properties	268:395	However, the combined influence of high-molecular-weight glutenin subunits (HMW-GS) and starch on dough mixing and rheological properties requires elucidation.
34808780	6	52	theme	specific	1037:1044	arg1	range					1046:1050	a specific range	1035:1050	a specific range of B-type starch proportion	1035:1078	Higher content of B-type starch strengthens interaction between starch and gluten skeleton at the dough heating stage, suggesting a specific range of B-type starch proportion can improve dough mixing characteristics.
34808780	4	53	theme	superior	587:594	arg1	alleles					603:609	superior HMW-GS alleles	587:609	superior HMW-GS alleles	587:609	Compared to ZZ 1 and ZZ 2, both SS 1 and SS 2 carried superior HMW-GS alleles, and exhibited extended dough development and stability time, indicating their significant dough mixing characteristics.
34808780	0	54	theme	wheat	59:63	arg1	dough					65:69	wheat dough	59:69	wheat dough mixing properties	59:87	Interaction of B-type starch with gluten skeleton improves wheat dough mixing properties by stabilizing gluten micro-structure.
34808780	2	55	theme	rheological	374:384	arg1	properties					386:395	rheological properties	374:395	rheological properties	374:395	However, the combined influence of high-molecular-weight glutenin subunits (HMW-GS) and starch on dough mixing and rheological properties requires elucidation.
32309938	9	0	from	ambiguity	1643:1651	arg1	spectra					1667:1673	the He-CTD spectra	1656:1673	the He-CTD spectra	1656:1673	Ion mobility spectrometry-mass spectrometry (IMS-MS) of the standards indicated that ambiguity in the He-CTD spectra was caused by isobaric impurities in the mixed-linked oligosaccharides.
32309938	10	1	theme	Radical-driven	1747:1760	arg1	dissociation					1762:1773	Radical-driven dissociation	1747:1773	Radical-driven dissociation induced by He-CTD	1747:1791	Radical-driven dissociation induced by He-CTD can thus expand MS/MS to carbohydrate linkage analysis, as demonstrated by the comprehensive fragment ion spectra on native oligosaccharides.
32309938	7	2	theme	sufficient	1280:1289	arg1	information					1291:1301	sufficient information to infer the connectivity specifically for each glycosidic bond	1280:1365	sufficient information to infer the connectivity specifically for each glycosidic bond	1280:1365	Overall, fragment ion spectra from He-CTD contained sufficient information to infer the connectivity specifically for each glycosidic bond.
32309938	2	3	theme	mass	258:261	arg1	spectrometry					263:274	tandem mass spectrometry	251:274	tandem mass spectrometry (MS/MS)	251:282	Although tandem mass spectrometry (MS/MS) often enables the compositional identification of carbohydrates, traditional MS/MS fragmentation methods fail to generate abundant cross-ring fragments of intrachain monosaccharides that could reveal carbohydrate connectivity.
32309938	2	3	theme	mass	258:261	arg1	MS/MS					277:281	MS/MS	277:281	MS/MS	277:281	Although tandem mass spectrometry (MS/MS) often enables the compositional identification of carbohydrates, traditional MS/MS fragmentation methods fail to generate abundant cross-ring fragments of intrachain monosaccharides that could reveal carbohydrate connectivity.
32309938	6	4	theme	various	1018:1024	arg1	standards					1026:1034	various standards	1018:1034	various standards	1018:1034	He-CTD of various standards up to a degree of polymerization of 7 showed that β-1,4- and β-1,3-linked carbohydrates can be distinguished based on diagnostic 3,5A fragment ions that are characteristic for β-1,4-linkages.
32309938	4	5	theme	oligosaccharide	752:766	arg1	precursors					768:777	isolated oligosaccharide precursors	743:777	isolated oligosaccharide precursors	743:777	In contrast to collision-induced dissociation (CID), He-CTD of isolated oligosaccharide precursors produced both glycosidic and cross-ring cleavages of each monosaccharide.
32309938	10	6	theme	carbohydrate	1818:1829	arg1	analysis					1839:1846	carbohydrate linkage analysis	1818:1846	carbohydrate linkage analysis	1818:1846	Radical-driven dissociation induced by He-CTD can thus expand MS/MS to carbohydrate linkage analysis, as demonstrated by the comprehensive fragment ion spectra on native oligosaccharides.
32309938	7	7	theme	fragment	1237:1244	arg1	spectra					1250:1256	fragment ion spectra	1237:1256	fragment ion spectra from He-CTD	1237:1268	Overall, fragment ion spectra from He-CTD contained sufficient information to infer the connectivity specifically for each glycosidic bond.
32309938	2	8	theme	traditional	349:359	arg1	methods					381:387	traditional MS/MS fragmentation methods	349:387	traditional MS/MS fragmentation methods	349:387	Although tandem mass spectrometry (MS/MS) often enables the compositional identification of carbohydrates, traditional MS/MS fragmentation methods fail to generate abundant cross-ring fragments of intrachain monosaccharides that could reveal carbohydrate connectivity.
32309938	6	9	theme	β-1,3-linked	1097:1108	arg1	carbohydrates					1110:1122	β-1,4- and β-1,3-linked carbohydrates	1086:1122	β-1,4- and β-1,3-linked carbohydrates	1086:1122	He-CTD of various standards up to a degree of polymerization of 7 showed that β-1,4- and β-1,3-linked carbohydrates can be distinguished based on diagnostic 3,5A fragment ions that are characteristic for β-1,4-linkages.
32309938	2	10	theme	tandem	251:256	arg1	spectrometry					263:274	tandem mass spectrometry	251:274	tandem mass spectrometry (MS/MS)	251:282	Although tandem mass spectrometry (MS/MS) often enables the compositional identification of carbohydrates, traditional MS/MS fragmentation methods fail to generate abundant cross-ring fragments of intrachain monosaccharides that could reveal carbohydrate connectivity.
32309938	2	10	theme	tandem	251:256	arg1	MS/MS					277:281	MS/MS	277:281	MS/MS	277:281	Although tandem mass spectrometry (MS/MS) often enables the compositional identification of carbohydrates, traditional MS/MS fragmentation methods fail to generate abundant cross-ring fragments of intrachain monosaccharides that could reveal carbohydrate connectivity.
32309938	8	11	theme	β-1,3-linkages	1423:1436	arg1	order					1403:1407	the order	1399:1407	the order of β-1,4- and β-1,3-linkages in mixed-linked oligosaccharide standards	1399:1478	When testing He-CTD to resolve the order of β-1,4- and β-1,3-linkages in mixed-linked oligosaccharide standards, He-CTD spectra sometimes provided less confident assignment of connectivity.
32309938	11	12	theme	precursors	2032:2041	arg1	separation					2009:2018	the separation	2005:2018	the separation of isobaric precursors	2005:2041	The determination of connectivity in true unknowns would benefit from the separation of isobaric precursors, through UPLC or IMS, before linkage determination via He-CTD.
32309938	2	13	theme	carbohydrates	334:346	arg1	identification					316:329	the compositional identification	298:329	the compositional identification of carbohydrates	298:346	Although tandem mass spectrometry (MS/MS) often enables the compositional identification of carbohydrates, traditional MS/MS fragmentation methods fail to generate abundant cross-ring fragments of intrachain monosaccharides that could reveal carbohydrate connectivity.
32309938	1	14	theme	carbohydrates	227:239	arg1	structure					177:185	structure	177:185	structure	177:185	The connection between monosaccharides influences the structure, solubility, and biological function of carbohydrates.
32309938	1	14	theme	carbohydrates	227:239	arg1	function					215:222	biological function	204:222	biological function	204:222	The connection between monosaccharides influences the structure, solubility, and biological function of carbohydrates.
32309938	1	14	theme	carbohydrates	227:239	arg1	solubility					188:197	solubility	188:197	solubility	188:197	The connection between monosaccharides influences the structure, solubility, and biological function of carbohydrates.
32309938	6	15	theme	β-1,4-	1086:1091	arg1	carbohydrates					1110:1122	β-1,4- and β-1,3-linked carbohydrates	1086:1122	β-1,4- and β-1,3-linked carbohydrates	1086:1122	He-CTD of various standards up to a degree of polymerization of 7 showed that β-1,4- and β-1,3-linked carbohydrates can be distinguished based on diagnostic 3,5A fragment ions that are characteristic for β-1,4-linkages.
32309938	8	16	theme	mixed-linked	1441:1452	arg1	standards					1470:1478	mixed-linked oligosaccharide standards	1441:1478	mixed-linked oligosaccharide standards	1441:1478	When testing He-CTD to resolve the order of β-1,4- and β-1,3-linkages in mixed-linked oligosaccharide standards, He-CTD spectra sometimes provided less confident assignment of connectivity.
32309938	5	17	theme	structural	981:990	arg1	interpretation					992:1005	structural interpretation	981:1005	structural interpretation	981:1005	The radical-driven dissociation in He-CTD induced single cleavage events, without consecutive fragmentations, which facilitated structural interpretation.
32309938	4	18	theme	cross-ring	808:817	arg1	cleavages					819:827	both glycosidic and cross-ring cleavages	788:827	both glycosidic and cross-ring cleavages of each monosaccharide	788:850	In contrast to collision-induced dissociation (CID), He-CTD of isolated oligosaccharide precursors produced both glycosidic and cross-ring cleavages of each monosaccharide.
32309938	4	19	dep	dissociation	713:724	arg1	contrast					683:690	contrast	683:690	contrast	683:690	In contrast to collision-induced dissociation (CID), He-CTD of isolated oligosaccharide precursors produced both glycosidic and cross-ring cleavages of each monosaccharide.
32309938	3	20	theme	oligosaccharides	662:677	arg1	connectivity					622:633	the connectivity	618:633	the connectivity of β-1,4- and β-1,3-linked oligosaccharides	618:677	We examined the potential of helium-charge transfer dissociation (He-CTD) as a method of MS/MS to decipher the connectivity of β-1,4- and β-1,3-linked oligosaccharides.
32309938	8	21	from	order	1403:1407	arg1	standards					1470:1478	mixed-linked oligosaccharide standards	1441:1478	mixed-linked oligosaccharide standards	1441:1478	When testing He-CTD to resolve the order of β-1,4- and β-1,3-linkages in mixed-linked oligosaccharide standards, He-CTD spectra sometimes provided less confident assignment of connectivity.
32309938	9	22	theme	isobaric	1689:1696	arg1	impurities					1698:1707	isobaric impurities	1689:1707	isobaric impurities in the mixed-linked oligosaccharides	1689:1744	Ion mobility spectrometry-mass spectrometry (IMS-MS) of the standards indicated that ambiguity in the He-CTD spectra was caused by isobaric impurities in the mixed-linked oligosaccharides.
32309938	8	23	theme	He-CTD	1481:1486	arg1	spectra					1488:1494	He-CTD spectra	1481:1494	He-CTD spectra	1481:1494	When testing He-CTD to resolve the order of β-1,4- and β-1,3-linkages in mixed-linked oligosaccharide standards, He-CTD spectra sometimes provided less confident assignment of connectivity.
32309938	6	24	theme	3,5A	1165:1168	arg1	ions					1179:1182	diagnostic 3,5A fragment ions	1154:1182	diagnostic 3,5A fragment ions that are characteristic for β-1,4-linkages	1154:1225	He-CTD of various standards up to a degree of polymerization of 7 showed that β-1,4- and β-1,3-linked carbohydrates can be distinguished based on diagnostic 3,5A fragment ions that are characteristic for β-1,4-linkages.
32309938	10	25	theme	native	1910:1915	arg1	oligosaccharides					1917:1932	native oligosaccharides	1910:1932	native oligosaccharides	1910:1932	Radical-driven dissociation induced by He-CTD can thus expand MS/MS to carbohydrate linkage analysis, as demonstrated by the comprehensive fragment ion spectra on native oligosaccharides.
32309938	9	26	theme	mixed-linked	1716:1727	arg1	oligosaccharides					1729:1744	the mixed-linked oligosaccharides	1712:1744	the mixed-linked oligosaccharides	1712:1744	Ion mobility spectrometry-mass spectrometry (IMS-MS) of the standards indicated that ambiguity in the He-CTD spectra was caused by isobaric impurities in the mixed-linked oligosaccharides.
32309938	4	27	theme	glycosidic	793:802	arg1	cleavages					819:827	both glycosidic and cross-ring cleavages	788:827	both glycosidic and cross-ring cleavages of each monosaccharide	788:850	In contrast to collision-induced dissociation (CID), He-CTD of isolated oligosaccharide precursors produced both glycosidic and cross-ring cleavages of each monosaccharide.
32309938	0	28	theme	Transfer	82:89	arg1	Spectrometry					109:120	Charge Transfer Dissociation Mass Spectrometry	75:120	Charge Transfer Dissociation Mass Spectrometry	75:120	Discrimination of β-1,4- and β-1,3-Linkages in Native Oligosaccharides via Charge Transfer Dissociation Mass Spectrometry.
32309938	7	29	contain	contained	1270:1278	arg2	information					1291:1301	sufficient information to infer the connectivity specifically for each glycosidic bond	1280:1365	sufficient information to infer the connectivity specifically for each glycosidic bond	1280:1365	Overall, fragment ion spectra from He-CTD contained sufficient information to infer the connectivity specifically for each glycosidic bond.
32309938	7	29	contain	contained	1270:1278	arg1	spectra					1250:1256	fragment ion spectra	1237:1256	fragment ion spectra from He-CTD	1237:1268	Overall, fragment ion spectra from He-CTD contained sufficient information to infer the connectivity specifically for each glycosidic bond.
32309938	0	30	theme	Mass	104:107	arg1	Spectrometry					109:120	Charge Transfer Dissociation Mass Spectrometry	75:120	Charge Transfer Dissociation Mass Spectrometry	75:120	Discrimination of β-1,4- and β-1,3-Linkages in Native Oligosaccharides via Charge Transfer Dissociation Mass Spectrometry.
32309938	3	31	theme	transfer	554:561	arg1	He-CTD					577:582	He-CTD	577:582	He-CTD	577:582	We examined the potential of helium-charge transfer dissociation (He-CTD) as a method of MS/MS to decipher the connectivity of β-1,4- and β-1,3-linked oligosaccharides.
32309938	3	31	theme	transfer	554:561	arg1	dissociation					563:574	helium-charge transfer dissociation	540:574	helium-charge transfer dissociation (He-CTD)	540:583	We examined the potential of helium-charge transfer dissociation (He-CTD) as a method of MS/MS to decipher the connectivity of β-1,4- and β-1,3-linked oligosaccharides.
32309938	8	32	theme	connectivity	1544:1555	arg1	assignment					1530:1539	less confident assignment	1515:1539	less confident assignment of connectivity	1515:1555	When testing He-CTD to resolve the order of β-1,4- and β-1,3-linkages in mixed-linked oligosaccharide standards, He-CTD spectra sometimes provided less confident assignment of connectivity.
32309938	10	33	theme	fragment	1886:1893	arg1	spectra					1899:1905	the comprehensive fragment ion spectra	1868:1905	the comprehensive fragment ion spectra on native oligosaccharides	1868:1932	Radical-driven dissociation induced by He-CTD can thus expand MS/MS to carbohydrate linkage analysis, as demonstrated by the comprehensive fragment ion spectra on native oligosaccharides.
32309938	0	34	theme	Charge	75:80	arg1	Spectrometry					109:120	Charge Transfer Dissociation Mass Spectrometry	75:120	Charge Transfer Dissociation Mass Spectrometry	75:120	Discrimination of β-1,4- and β-1,3-Linkages in Native Oligosaccharides via Charge Transfer Dissociation Mass Spectrometry.
32309938	4	35	theme	monosaccharide	837:850	arg1	cleavages					819:827	both glycosidic and cross-ring cleavages	788:827	both glycosidic and cross-ring cleavages of each monosaccharide	788:850	In contrast to collision-induced dissociation (CID), He-CTD of isolated oligosaccharide precursors produced both glycosidic and cross-ring cleavages of each monosaccharide.
32309938	2	36	theme	intrachain	439:448	arg1	monosaccharides					450:464	intrachain monosaccharides	439:464	intrachain monosaccharides	439:464	Although tandem mass spectrometry (MS/MS) often enables the compositional identification of carbohydrates, traditional MS/MS fragmentation methods fail to generate abundant cross-ring fragments of intrachain monosaccharides that could reveal carbohydrate connectivity.
32309938	3	37	theme	helium-charge	540:552	arg1	He-CTD					577:582	He-CTD	577:582	He-CTD	577:582	We examined the potential of helium-charge transfer dissociation (He-CTD) as a method of MS/MS to decipher the connectivity of β-1,4- and β-1,3-linked oligosaccharides.
32309938	3	37	theme	helium-charge	540:552	arg1	dissociation					563:574	helium-charge transfer dissociation	540:574	helium-charge transfer dissociation (He-CTD)	540:583	We examined the potential of helium-charge transfer dissociation (He-CTD) as a method of MS/MS to decipher the connectivity of β-1,4- and β-1,3-linked oligosaccharides.
32309938	0	38	theme	β-1,4-	18:23	arg1	Discrimination					0:13	Discrimination	0:13	Discrimination of β-1,4-	0:23	Discrimination of β-1,4- and β-1,3-Linkages in Native Oligosaccharides via Charge Transfer Dissociation Mass Spectrometry.
32309938	3	39	theme	MS/MS	600:604	arg1	method					590:595	a method	588:595	a method of MS/MS	588:604	We examined the potential of helium-charge transfer dissociation (He-CTD) as a method of MS/MS to decipher the connectivity of β-1,4- and β-1,3-linked oligosaccharides.
32309938	3	39	theme	MS/MS	600:604	arg1	potential					527:535	the potential	523:535	the potential of helium-charge transfer dissociation (He-CTD)	523:583	We examined the potential of helium-charge transfer dissociation (He-CTD) as a method of MS/MS to decipher the connectivity of β-1,4- and β-1,3-linked oligosaccharides.
32309938	3	40	link	β-1,3-linked	649:660	arg1	oligosaccharides					662:677	β-1,4- and β-1,3-linked oligosaccharides	638:677	β-1,4- and β-1,3-linked oligosaccharides	638:677	We examined the potential of helium-charge transfer dissociation (He-CTD) as a method of MS/MS to decipher the connectivity of β-1,4- and β-1,3-linked oligosaccharides.
32309938	5	41	theme	single	903:908	arg1	events					919:924	single cleavage events	903:924	single cleavage events	903:924	The radical-driven dissociation in He-CTD induced single cleavage events, without consecutive fragmentations, which facilitated structural interpretation.
32309938	9	42	link	mixed-linked	1716:1727	arg1	oligosaccharides					1729:1744	the mixed-linked oligosaccharides	1712:1744	the mixed-linked oligosaccharides	1712:1744	Ion mobility spectrometry-mass spectrometry (IMS-MS) of the standards indicated that ambiguity in the He-CTD spectra was caused by isobaric impurities in the mixed-linked oligosaccharides.
32309938	7	43	from	He-CTD	1263:1268	arg1	spectra					1250:1256	fragment ion spectra	1237:1256	fragment ion spectra from He-CTD	1237:1268	Overall, fragment ion spectra from He-CTD contained sufficient information to infer the connectivity specifically for each glycosidic bond.
32309938	0	44	theme	Native	47:52	arg1	Oligosaccharides					54:69	Native Oligosaccharides	47:69	Native Oligosaccharides	47:69	Discrimination of β-1,4- and β-1,3-Linkages in Native Oligosaccharides via Charge Transfer Dissociation Mass Spectrometry.
32309938	9	45	theme	mobility	1562:1569	arg1	IMS-MS					1603:1608	IMS-MS	1603:1608	IMS-MS	1603:1608	Ion mobility spectrometry-mass spectrometry (IMS-MS) of the standards indicated that ambiguity in the He-CTD spectra was caused by isobaric impurities in the mixed-linked oligosaccharides.
32309938	9	45	theme	mobility	1562:1569	arg1	spectrometry					1589:1600	Ion mobility spectrometry-mass spectrometry	1558:1600	Ion mobility spectrometry-mass spectrometry (IMS-MS) of the standards	1558:1626	Ion mobility spectrometry-mass spectrometry (IMS-MS) of the standards indicated that ambiguity in the He-CTD spectra was caused by isobaric impurities in the mixed-linked oligosaccharides.
32309938	8	46	link	mixed-linked	1441:1452	arg1	standards					1470:1478	mixed-linked oligosaccharide standards	1441:1478	mixed-linked oligosaccharide standards	1441:1478	When testing He-CTD to resolve the order of β-1,4- and β-1,3-linkages in mixed-linked oligosaccharide standards, He-CTD spectra sometimes provided less confident assignment of connectivity.
32309938	11	47	theme	true	1972:1975	arg1	unknowns					1977:1984	true unknowns	1972:1984	true unknowns	1972:1984	The determination of connectivity in true unknowns would benefit from the separation of isobaric precursors, through UPLC or IMS, before linkage determination via He-CTD.
32309938	3	48	theme	dissociation	563:574	arg1	method					590:595	a method	588:595	a method of MS/MS	588:604	We examined the potential of helium-charge transfer dissociation (He-CTD) as a method of MS/MS to decipher the connectivity of β-1,4- and β-1,3-linked oligosaccharides.
32309938	3	48	theme	dissociation	563:574	arg1	potential					527:535	the potential	523:535	the potential of helium-charge transfer dissociation (He-CTD)	523:583	We examined the potential of helium-charge transfer dissociation (He-CTD) as a method of MS/MS to decipher the connectivity of β-1,4- and β-1,3-linked oligosaccharides.
32309938	6	49	link	β-1,3-linked	1097:1108	arg1	carbohydrates					1110:1122	β-1,4- and β-1,3-linked carbohydrates	1086:1122	β-1,4- and β-1,3-linked carbohydrates	1086:1122	He-CTD of various standards up to a degree of polymerization of 7 showed that β-1,4- and β-1,3-linked carbohydrates can be distinguished based on diagnostic 3,5A fragment ions that are characteristic for β-1,4-linkages.
32309938	10	50	from	spectra	1899:1905	arg1	oligosaccharides					1917:1932	native oligosaccharides	1910:1932	native oligosaccharides	1910:1932	Radical-driven dissociation induced by He-CTD can thus expand MS/MS to carbohydrate linkage analysis, as demonstrated by the comprehensive fragment ion spectra on native oligosaccharides.
32309938	11	51	theme	connectivity	1956:1967	arg1	determination					1939:1951	The determination	1935:1951	The determination of connectivity in true unknowns	1935:1984	The determination of connectivity in true unknowns would benefit from the separation of isobaric precursors, through UPLC or IMS, before linkage determination via He-CTD.
32309938	6	52	theme	polymerization	1054:1067	arg1	degree					1044:1049	a degree	1042:1049	a degree of polymerization of 7	1042:1072	He-CTD of various standards up to a degree of polymerization of 7 showed that β-1,4- and β-1,3-linked carbohydrates can be distinguished based on diagnostic 3,5A fragment ions that are characteristic for β-1,4-linkages.
32309938	4	53	theme	collision-induced	695:711	arg1	CID					727:729	CID	727:729	CID	727:729	In contrast to collision-induced dissociation (CID), He-CTD of isolated oligosaccharide precursors produced both glycosidic and cross-ring cleavages of each monosaccharide.
32309938	4	53	theme	collision-induced	695:711	arg1	dissociation					713:724	collision-induced dissociation	695:724	collision-induced dissociation (CID)	695:730	In contrast to collision-induced dissociation (CID), He-CTD of isolated oligosaccharide precursors produced both glycosidic and cross-ring cleavages of each monosaccharide.
32309938	2	54	theme	abundant	406:413	arg1	fragments					426:434	abundant cross-ring fragments	406:434	abundant cross-ring fragments of intrachain monosaccharides that could reveal carbohydrate connectivity	406:508	Although tandem mass spectrometry (MS/MS) often enables the compositional identification of carbohydrates, traditional MS/MS fragmentation methods fail to generate abundant cross-ring fragments of intrachain monosaccharides that could reveal carbohydrate connectivity.
32309938	9	55	theme	standards	1618:1626	arg1	IMS-MS					1603:1608	IMS-MS	1603:1608	IMS-MS	1603:1608	Ion mobility spectrometry-mass spectrometry (IMS-MS) of the standards indicated that ambiguity in the He-CTD spectra was caused by isobaric impurities in the mixed-linked oligosaccharides.
32309938	9	55	theme	standards	1618:1626	arg1	spectrometry					1589:1600	Ion mobility spectrometry-mass spectrometry	1558:1600	Ion mobility spectrometry-mass spectrometry (IMS-MS) of the standards	1558:1626	Ion mobility spectrometry-mass spectrometry (IMS-MS) of the standards indicated that ambiguity in the He-CTD spectra was caused by isobaric impurities in the mixed-linked oligosaccharides.
32309938	3	56	theme	β-1,4-	638:643	arg1	oligosaccharides					662:677	β-1,4- and β-1,3-linked oligosaccharides	638:677	β-1,4- and β-1,3-linked oligosaccharides	638:677	We examined the potential of helium-charge transfer dissociation (He-CTD) as a method of MS/MS to decipher the connectivity of β-1,4- and β-1,3-linked oligosaccharides.
32309938	7	57	theme	ion	1246:1248	arg1	spectra					1250:1256	fragment ion spectra	1237:1256	fragment ion spectra from He-CTD	1237:1268	Overall, fragment ion spectra from He-CTD contained sufficient information to infer the connectivity specifically for each glycosidic bond.
32309938	2	58	theme	compositional	302:314	arg1	identification					316:329	the compositional identification	298:329	the compositional identification of carbohydrates	298:346	Although tandem mass spectrometry (MS/MS) often enables the compositional identification of carbohydrates, traditional MS/MS fragmentation methods fail to generate abundant cross-ring fragments of intrachain monosaccharides that could reveal carbohydrate connectivity.
32309938	8	59	theme	β-1,4-	1412:1417	arg1	order					1403:1407	the order	1399:1407	the order of β-1,4- and β-1,3-linkages in mixed-linked oligosaccharide standards	1399:1478	When testing He-CTD to resolve the order of β-1,4- and β-1,3-linkages in mixed-linked oligosaccharide standards, He-CTD spectra sometimes provided less confident assignment of connectivity.
32309938	6	60	theme	standards	1026:1034	arg1	He-CTD					1008:1013	He-CTD	1008:1013	He-CTD of various standards up to a degree of polymerization of 7	1008:1072	He-CTD of various standards up to a degree of polymerization of 7 showed that β-1,4- and β-1,3-linked carbohydrates can be distinguished based on diagnostic 3,5A fragment ions that are characteristic for β-1,4-linkages.
32309938	9	61	from	impurities	1698:1707	arg1	oligosaccharides					1729:1744	the mixed-linked oligosaccharides	1712:1744	the mixed-linked oligosaccharides	1712:1744	Ion mobility spectrometry-mass spectrometry (IMS-MS) of the standards indicated that ambiguity in the He-CTD spectra was caused by isobaric impurities in the mixed-linked oligosaccharides.
32309938	10	62	theme	linkage	1831:1837	arg1	analysis					1839:1846	carbohydrate linkage analysis	1818:1846	carbohydrate linkage analysis	1818:1846	Radical-driven dissociation induced by He-CTD can thus expand MS/MS to carbohydrate linkage analysis, as demonstrated by the comprehensive fragment ion spectra on native oligosaccharides.
32309938	2	63	theme	MS/MS	361:365	arg1	methods					381:387	traditional MS/MS fragmentation methods	349:387	traditional MS/MS fragmentation methods	349:387	Although tandem mass spectrometry (MS/MS) often enables the compositional identification of carbohydrates, traditional MS/MS fragmentation methods fail to generate abundant cross-ring fragments of intrachain monosaccharides that could reveal carbohydrate connectivity.
32309938	1	64	theme	biological	204:213	arg1	function					215:222	biological function	204:222	biological function	204:222	The connection between monosaccharides influences the structure, solubility, and biological function of carbohydrates.
32309938	4	65	theme	isolated	743:750	arg1	precursors					768:777	isolated oligosaccharide precursors	743:777	isolated oligosaccharide precursors	743:777	In contrast to collision-induced dissociation (CID), He-CTD of isolated oligosaccharide precursors produced both glycosidic and cross-ring cleavages of each monosaccharide.
32309938	9	66	theme	He-CTD	1660:1665	arg1	spectra					1667:1673	the He-CTD spectra	1656:1673	the He-CTD spectra	1656:1673	Ion mobility spectrometry-mass spectrometry (IMS-MS) of the standards indicated that ambiguity in the He-CTD spectra was caused by isobaric impurities in the mixed-linked oligosaccharides.
32309938	3	67	theme	β-1,3-linked	649:660	arg1	oligosaccharides					662:677	β-1,4- and β-1,3-linked oligosaccharides	638:677	β-1,4- and β-1,3-linked oligosaccharides	638:677	We examined the potential of helium-charge transfer dissociation (He-CTD) as a method of MS/MS to decipher the connectivity of β-1,4- and β-1,3-linked oligosaccharides.
32309938	6	68	theme	7	1072:1072	arg1	polymerization					1054:1067	polymerization	1054:1067	polymerization of 7	1054:1072	He-CTD of various standards up to a degree of polymerization of 7 showed that β-1,4- and β-1,3-linked carbohydrates can be distinguished based on diagnostic 3,5A fragment ions that are characteristic for β-1,4-linkages.
32309938	8	69	theme	oligosaccharide	1454:1468	arg1	standards					1470:1478	mixed-linked oligosaccharide standards	1441:1478	mixed-linked oligosaccharide standards	1441:1478	When testing He-CTD to resolve the order of β-1,4- and β-1,3-linkages in mixed-linked oligosaccharide standards, He-CTD spectra sometimes provided less confident assignment of connectivity.
32309938	6	70	theme	fragment	1170:1177	arg1	ions					1179:1182	diagnostic 3,5A fragment ions	1154:1182	diagnostic 3,5A fragment ions that are characteristic for β-1,4-linkages	1154:1225	He-CTD of various standards up to a degree of polymerization of 7 showed that β-1,4- and β-1,3-linked carbohydrates can be distinguished based on diagnostic 3,5A fragment ions that are characteristic for β-1,4-linkages.
32309938	11	71	theme	linkage	2072:2078	arg1	determination					2080:2092	linkage determination	2072:2092	linkage determination via He-CTD	2072:2103	The determination of connectivity in true unknowns would benefit from the separation of isobaric precursors, through UPLC or IMS, before linkage determination via He-CTD.
32309938	5	72	theme	radical-driven	857:870	arg1	dissociation					872:883	The radical-driven dissociation	853:883	The radical-driven dissociation in He-CTD	853:893	The radical-driven dissociation in He-CTD induced single cleavage events, without consecutive fragmentations, which facilitated structural interpretation.
32309938	5	73	from	dissociation	872:883	arg1	He-CTD					888:893	He-CTD	888:893	He-CTD	888:893	The radical-driven dissociation in He-CTD induced single cleavage events, without consecutive fragmentations, which facilitated structural interpretation.
32309938	6	74	theme	diagnostic	1154:1163	arg1	ions					1179:1182	diagnostic 3,5A fragment ions	1154:1182	diagnostic 3,5A fragment ions that are characteristic for β-1,4-linkages	1154:1225	He-CTD of various standards up to a degree of polymerization of 7 showed that β-1,4- and β-1,3-linked carbohydrates can be distinguished based on diagnostic 3,5A fragment ions that are characteristic for β-1,4-linkages.
32309938	4	75	theme	precursors	768:777	arg1	He-CTD					733:738	He-CTD	733:738	He-CTD of isolated oligosaccharide precursors	733:777	In contrast to collision-induced dissociation (CID), He-CTD of isolated oligosaccharide precursors produced both glycosidic and cross-ring cleavages of each monosaccharide.
32309938	0	76	theme	Dissociation	91:102	arg1	Spectrometry					109:120	Charge Transfer Dissociation Mass Spectrometry	75:120	Charge Transfer Dissociation Mass Spectrometry	75:120	Discrimination of β-1,4- and β-1,3-Linkages in Native Oligosaccharides via Charge Transfer Dissociation Mass Spectrometry.
32309938	11	77	theme	isobaric	2023:2030	arg1	precursors					2032:2041	isobaric precursors	2023:2041	isobaric precursors	2023:2041	The determination of connectivity in true unknowns would benefit from the separation of isobaric precursors, through UPLC or IMS, before linkage determination via He-CTD.
32309938	8	78	theme	confident	1520:1528	arg1	assignment					1530:1539	less confident assignment	1515:1539	less confident assignment of connectivity	1515:1555	When testing He-CTD to resolve the order of β-1,4- and β-1,3-linkages in mixed-linked oligosaccharide standards, He-CTD spectra sometimes provided less confident assignment of connectivity.
32309938	7	79	theme	glycosidic	1351:1360	arg1	bond					1362:1365	each glycosidic bond	1346:1365	each glycosidic bond	1346:1365	Overall, fragment ion spectra from He-CTD contained sufficient information to infer the connectivity specifically for each glycosidic bond.
32309938	11	80	from	determination	1939:1951	arg1	unknowns					1977:1984	true unknowns	1972:1984	true unknowns	1972:1984	The determination of connectivity in true unknowns would benefit from the separation of isobaric precursors, through UPLC or IMS, before linkage determination via He-CTD.
32309938	2	81	theme	monosaccharides	450:464	arg1	fragments					426:434	abundant cross-ring fragments	406:434	abundant cross-ring fragments of intrachain monosaccharides that could reveal carbohydrate connectivity	406:508	Although tandem mass spectrometry (MS/MS) often enables the compositional identification of carbohydrates, traditional MS/MS fragmentation methods fail to generate abundant cross-ring fragments of intrachain monosaccharides that could reveal carbohydrate connectivity.
32309938	5	82	theme	consecutive	935:945	arg1	fragmentations					947:960	consecutive fragmentations	935:960	consecutive fragmentations	935:960	The radical-driven dissociation in He-CTD induced single cleavage events, without consecutive fragmentations, which facilitated structural interpretation.
32309938	1	83	dep	structure	177:185	arg1	the					173:175	the	173:175	the	173:175	The connection between monosaccharides influences the structure, solubility, and biological function of carbohydrates.
32309938	2	84	theme	carbohydrate	484:495	arg1	connectivity					497:508	carbohydrate connectivity	484:508	carbohydrate connectivity	484:508	Although tandem mass spectrometry (MS/MS) often enables the compositional identification of carbohydrates, traditional MS/MS fragmentation methods fail to generate abundant cross-ring fragments of intrachain monosaccharides that could reveal carbohydrate connectivity.
32309938	10	85	theme	ion	1895:1897	arg1	spectra					1899:1905	the comprehensive fragment ion spectra	1868:1905	the comprehensive fragment ion spectra on native oligosaccharides	1868:1932	Radical-driven dissociation induced by He-CTD can thus expand MS/MS to carbohydrate linkage analysis, as demonstrated by the comprehensive fragment ion spectra on native oligosaccharides.
32309938	5	86	theme	cleavage	910:917	arg1	events					919:924	single cleavage events	903:924	single cleavage events	903:924	The radical-driven dissociation in He-CTD induced single cleavage events, without consecutive fragmentations, which facilitated structural interpretation.
32309938	9	87	theme	Ion	1558:1560	arg1	IMS-MS					1603:1608	IMS-MS	1603:1608	IMS-MS	1603:1608	Ion mobility spectrometry-mass spectrometry (IMS-MS) of the standards indicated that ambiguity in the He-CTD spectra was caused by isobaric impurities in the mixed-linked oligosaccharides.
32309938	9	87	theme	Ion	1558:1560	arg1	spectrometry					1589:1600	Ion mobility spectrometry-mass spectrometry	1558:1600	Ion mobility spectrometry-mass spectrometry (IMS-MS) of the standards	1558:1626	Ion mobility spectrometry-mass spectrometry (IMS-MS) of the standards indicated that ambiguity in the He-CTD spectra was caused by isobaric impurities in the mixed-linked oligosaccharides.
32309938	9	88	theme	spectrometry-mass	1571:1587	arg1	IMS-MS					1603:1608	IMS-MS	1603:1608	IMS-MS	1603:1608	Ion mobility spectrometry-mass spectrometry (IMS-MS) of the standards indicated that ambiguity in the He-CTD spectra was caused by isobaric impurities in the mixed-linked oligosaccharides.
32309938	9	88	theme	spectrometry-mass	1571:1587	arg1	spectrometry					1589:1600	Ion mobility spectrometry-mass spectrometry	1558:1600	Ion mobility spectrometry-mass spectrometry (IMS-MS) of the standards	1558:1626	Ion mobility spectrometry-mass spectrometry (IMS-MS) of the standards indicated that ambiguity in the He-CTD spectra was caused by isobaric impurities in the mixed-linked oligosaccharides.
32309938	2	89	theme	fragmentation	367:379	arg1	methods					381:387	traditional MS/MS fragmentation methods	349:387	traditional MS/MS fragmentation methods	349:387	Although tandem mass spectrometry (MS/MS) often enables the compositional identification of carbohydrates, traditional MS/MS fragmentation methods fail to generate abundant cross-ring fragments of intrachain monosaccharides that could reveal carbohydrate connectivity.
32309938	10	90	theme	comprehensive	1872:1884	arg1	spectra					1899:1905	the comprehensive fragment ion spectra	1868:1905	the comprehensive fragment ion spectra on native oligosaccharides	1868:1932	Radical-driven dissociation induced by He-CTD can thus expand MS/MS to carbohydrate linkage analysis, as demonstrated by the comprehensive fragment ion spectra on native oligosaccharides.
32309938	2	91	theme	cross-ring	415:424	arg1	fragments					426:434	abundant cross-ring fragments	406:434	abundant cross-ring fragments of intrachain monosaccharides that could reveal carbohydrate connectivity	406:508	Although tandem mass spectrometry (MS/MS) often enables the compositional identification of carbohydrates, traditional MS/MS fragmentation methods fail to generate abundant cross-ring fragments of intrachain monosaccharides that could reveal carbohydrate connectivity.
34852446	3	0	theme	acid	747:750	arg1	concentration					752:764	1.21% acid concentration	741:764	1.21% acid concentration	741:764	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	3	1	theme	essential	683:691	arg1	oils					693:696	essential oils	683:696	essential oils	683:696	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	1	2	theme	orange	154:159	arg1	biorefinery					172:182	An orange peel waste biorefinery	151:182	An orange peel waste biorefinery	151:182	An orange peel waste biorefinery was developed employing a design of experiments approach to optimize the ultrasound-assisted dilute acid hydrolysis process applied for production of useful commodities.
34852446	4	3	theme	%	822:822	arg1	sugars					871:876	0.12% w/w essential oils, 45% w/w pectin and 40% w/w sugars	818:876	0.12% w/w essential oils, 45% w/w pectin and 40% w/w sugars	818:876	Maximum production yields of 0.12% w/w essential oils, 45% w/w pectin and 40% w/w sugars were achieved under optimized conditions in lab- and pilot-scale facilities.
34852446	4	4	theme	pilot-scale	931:941	arg1	facilities					943:952	lab- and pilot-scale facilities	922:952	lab- and pilot-scale facilities	922:952	Maximum production yields of 0.12% w/w essential oils, 45% w/w pectin and 40% w/w sugars were achieved under optimized conditions in lab- and pilot-scale facilities.
34852446	1	5	theme	peel	161:164	arg1	biorefinery					172:182	An orange peel waste biorefinery	151:182	An orange peel waste biorefinery	151:182	An orange peel waste biorefinery was developed employing a design of experiments approach to optimize the ultrasound-assisted dilute acid hydrolysis process applied for production of useful commodities.
34852446	1	6	theme	ultrasound-assisted	257:275	arg1	process					300:306	the ultrasound-assisted dilute acid hydrolysis process	253:306	the ultrasound-assisted dilute acid hydrolysis process applied for production of useful commodities	253:351	An orange peel waste biorefinery was developed employing a design of experiments approach to optimize the ultrasound-assisted dilute acid hydrolysis process applied for production of useful commodities.
34852446	3	7	theme	1.21	741:744	arg1	%					745:745	%	745:745	%	745:745	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	4	8	theme	optimized	898:906	arg1	conditions					908:917	optimized conditions	898:917	optimized conditions	898:917	Maximum production yields of 0.12% w/w essential oils, 45% w/w pectin and 40% w/w sugars were achieved under optimized conditions in lab- and pilot-scale facilities.
34852446	3	9	theme	function	594:601	arg1	Application					564:574	Application	564:574	Application of a desirability function	564:601	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	3	10	theme	optimal	618:624	arg1	conditions					626:635	the optimal conditions	614:635	the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration	614:786	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	1	11	theme	dilute	277:282	arg1	process					300:306	the ultrasound-assisted dilute acid hydrolysis process	253:306	the ultrasound-assisted dilute acid hydrolysis process applied for production of useful commodities	253:351	An orange peel waste biorefinery was developed employing a design of experiments approach to optimize the ultrasound-assisted dilute acid hydrolysis process applied for production of useful commodities.
34852446	0	12	theme	bacterial	88:96	arg1	cellulose					98:106	bacterial cellulose	88:106	bacterial cellulose	88:106	Ultrasound-assisted dilute acid hydrolysis for production of essential oils, pectin and bacterial cellulose via a citrus processing waste biorefinery.
34852446	0	12	theme	bacterial	88:96	arg1	hydrolysis					32:41	Ultrasound-assisted dilute acid hydrolysis	0:41	Ultrasound-assisted dilute acid hydrolysis for production of essential oils	0:74	Ultrasound-assisted dilute acid hydrolysis for production of essential oils, pectin and bacterial cellulose via a citrus processing waste biorefinery.
34852446	3	13	theme	34.2 min	770:777	arg1	duration					779:786	34.2 min duration	770:786	34.2 min duration	770:786	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	5	14	theme	bacterial	993:1001	arg1	fermentations					1013:1025	bacterial cellulose fermentations	993:1025	bacterial cellulose fermentations producing 5.82 g biopolymer per 100 g waste	993:1069	The hydrolyzate formed was applied in bacterial cellulose fermentations producing 5.82 g biopolymer per 100 g waste.
34852446	2	15	theme	surface	394:400	arg1	methodology					402:412	Central composite design-based response surface methodology	354:412	Central composite design-based response surface methodology	354:412	Central composite design-based response surface methodology was used to approximate the combined effects of process parameters in simultaneous production of essential oils, pectin and a sugar-rich hydrolyzate.
34852446	1	16	theme	acid	284:287	arg1	process					300:306	the ultrasound-assisted dilute acid hydrolysis process	253:306	the ultrasound-assisted dilute acid hydrolysis process applied for production of useful commodities	253:351	An orange peel waste biorefinery was developed employing a design of experiments approach to optimize the ultrasound-assisted dilute acid hydrolysis process applied for production of useful commodities.
34852446	4	17	theme	%	865:865	arg1	sugars					871:876	0.12% w/w essential oils, 45% w/w pectin and 40% w/w sugars	818:876	0.12% w/w essential oils, 45% w/w pectin and 40% w/w sugars	818:876	Maximum production yields of 0.12% w/w essential oils, 45% w/w pectin and 40% w/w sugars were achieved under optimized conditions in lab- and pilot-scale facilities.
34852446	5	18	theme	cellulose	1003:1011	arg1	fermentations					1013:1025	bacterial cellulose fermentations	993:1025	bacterial cellulose fermentations producing 5.82 g biopolymer per 100 g waste	993:1069	The hydrolyzate formed was applied in bacterial cellulose fermentations producing 5.82 g biopolymer per 100 g waste.
34852446	1	19	theme	waste	166:170	arg1	biorefinery					172:182	An orange peel waste biorefinery	151:182	An orange peel waste biorefinery	151:182	An orange peel waste biorefinery was developed employing a design of experiments approach to optimize the ultrasound-assisted dilute acid hydrolysis process applied for production of useful commodities.
34852446	2	20	theme	simultaneous	484:495	arg1	production					497:506	simultaneous production	484:506	simultaneous production of essential oils	484:524	Central composite design-based response surface methodology was used to approximate the combined effects of process parameters in simultaneous production of essential oils, pectin and a sugar-rich hydrolyzate.
34852446	0	21	theme	dilute	20:25	arg1	pectin					77:82	pectin	77:82	pectin	77:82	Ultrasound-assisted dilute acid hydrolysis for production of essential oils, pectin and bacterial cellulose via a citrus processing waste biorefinery.
34852446	0	21	theme	dilute	20:25	arg1	cellulose					98:106	bacterial cellulose	88:106	bacterial cellulose	88:106	Ultrasound-assisted dilute acid hydrolysis for production of essential oils, pectin and bacterial cellulose via a citrus processing waste biorefinery.
34852446	0	21	theme	dilute	20:25	arg1	hydrolysis					32:41	Ultrasound-assisted dilute acid hydrolysis	0:41	Ultrasound-assisted dilute acid hydrolysis for production of essential oils	0:74	Ultrasound-assisted dilute acid hydrolysis for production of essential oils, pectin and bacterial cellulose via a citrus processing waste biorefinery.
34852446	4	22	theme	lab-	922:925	arg1	facilities					943:952	lab- and pilot-scale facilities	922:952	lab- and pilot-scale facilities	922:952	Maximum production yields of 0.12% w/w essential oils, 45% w/w pectin and 40% w/w sugars were achieved under optimized conditions in lab- and pilot-scale facilities.
34852446	4	23	theme	Maximum	789:795	arg1	production					797:806	Maximum production	789:806	Maximum production	789:806	Maximum production yields of 0.12% w/w essential oils, 45% w/w pectin and 40% w/w sugars were achieved under optimized conditions in lab- and pilot-scale facilities.
34852446	6	24	theme	process	1112:1118	arg1	analysis					1120:1127	process analysis	1112:1127	process analysis	1112:1127	Design of experiments was efficient for process analysis and optimization providing a systems platform for the study of biomass-based biorefineries.
34852446	0	25	theme	Ultrasound-assisted	0:18	arg1	pectin					77:82	pectin	77:82	pectin	77:82	Ultrasound-assisted dilute acid hydrolysis for production of essential oils, pectin and bacterial cellulose via a citrus processing waste biorefinery.
34852446	0	25	theme	Ultrasound-assisted	0:18	arg1	cellulose					98:106	bacterial cellulose	88:106	bacterial cellulose	88:106	Ultrasound-assisted dilute acid hydrolysis for production of essential oils, pectin and bacterial cellulose via a citrus processing waste biorefinery.
34852446	0	25	theme	Ultrasound-assisted	0:18	arg1	hydrolysis					32:41	Ultrasound-assisted dilute acid hydrolysis	0:41	Ultrasound-assisted dilute acid hydrolysis for production of essential oils	0:74	Ultrasound-assisted dilute acid hydrolysis for production of essential oils, pectin and bacterial cellulose via a citrus processing waste biorefinery.
34852446	1	26	theme	hydrolysis	289:298	arg1	process					300:306	the ultrasound-assisted dilute acid hydrolysis process	253:306	the ultrasound-assisted dilute acid hydrolysis process applied for production of useful commodities	253:351	An orange peel waste biorefinery was developed employing a design of experiments approach to optimize the ultrasound-assisted dilute acid hydrolysis process applied for production of useful commodities.
34852446	4	27	theme	w/w	867:869	arg1	%					865:865	40% w/w	863:869	40% w/w	863:869	Maximum production yields of 0.12% w/w essential oils, 45% w/w pectin and 40% w/w sugars were achieved under optimized conditions in lab- and pilot-scale facilities.
34852446	5	28	theme	100 g	1059:1063	arg1	waste					1065:1069	100 g waste	1059:1069	100 g waste	1059:1069	The hydrolyzate formed was applied in bacterial cellulose fermentations producing 5.82 g biopolymer per 100 g waste.
34852446	5	29	theme	5.82 g	1037:1042	arg1	biopolymer					1044:1053	5.82 g biopolymer	1037:1053	5.82 g biopolymer per 100 g waste	1037:1069	The hydrolyzate formed was applied in bacterial cellulose fermentations producing 5.82 g biopolymer per 100 g waste.
34852446	0	30	theme	processing	121:130	arg1	biorefinery					138:148	a citrus processing waste biorefinery	112:148	a citrus processing waste biorefinery	112:148	Ultrasound-assisted dilute acid hydrolysis for production of essential oils, pectin and bacterial cellulose via a citrus processing waste biorefinery.
34852446	4	31	theme	pectin	852:857	arg1	sugars					871:876	0.12% w/w essential oils, 45% w/w pectin and 40% w/w sugars	818:876	0.12% w/w essential oils, 45% w/w pectin and 40% w/w sugars	818:876	Maximum production yields of 0.12% w/w essential oils, 45% w/w pectin and 40% w/w sugars were achieved under optimized conditions in lab- and pilot-scale facilities.
34852446	6	32	theme	biomass-based	1192:1204	arg1	biorefineries					1206:1218	biomass-based biorefineries	1192:1218	biomass-based biorefineries	1192:1218	Design of experiments was efficient for process analysis and optimization providing a systems platform for the study of biomass-based biorefineries.
34852446	4	33	theme	w/w	848:850	arg1	pectin					852:857	45% w/w pectin	844:857	45% w/w pectin	844:857	Maximum production yields of 0.12% w/w essential oils, 45% w/w pectin and 40% w/w sugars were achieved under optimized conditions in lab- and pilot-scale facilities.
34852446	2	34	theme	process	462:468	arg1	parameters					470:479	process parameters	462:479	process parameters	462:479	Central composite design-based response surface methodology was used to approximate the combined effects of process parameters in simultaneous production of essential oils, pectin and a sugar-rich hydrolyzate.
34852446	0	35	theme	acid	27:30	arg1	pectin					77:82	pectin	77:82	pectin	77:82	Ultrasound-assisted dilute acid hydrolysis for production of essential oils, pectin and bacterial cellulose via a citrus processing waste biorefinery.
34852446	0	35	theme	acid	27:30	arg1	cellulose					98:106	bacterial cellulose	88:106	bacterial cellulose	88:106	Ultrasound-assisted dilute acid hydrolysis for production of essential oils, pectin and bacterial cellulose via a citrus processing waste biorefinery.
34852446	0	35	theme	acid	27:30	arg1	hydrolysis					32:41	Ultrasound-assisted dilute acid hydrolysis	0:41	Ultrasound-assisted dilute acid hydrolysis for production of essential oils	0:74	Ultrasound-assisted dilute acid hydrolysis for production of essential oils, pectin and bacterial cellulose via a citrus processing waste biorefinery.
34852446	0	36	theme	citrus	114:119	arg1	biorefinery					138:148	a citrus processing waste biorefinery	112:148	a citrus processing waste biorefinery	112:148	Ultrasound-assisted dilute acid hydrolysis for production of essential oils, pectin and bacterial cellulose via a citrus processing waste biorefinery.
34852446	3	37	theme	sugars	710:715	arg1	efficiency					669:678	maximal production efficiency	650:678	maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading	650:738	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	3	37	theme	sugars	710:715	arg1	concentration					752:764	1.21% acid concentration	741:764	1.21% acid concentration	741:764	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	3	37	theme	sugars	710:715	arg1	duration					779:786	34.2 min duration	770:786	34.2 min duration	770:786	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	2	38	theme	response	385:392	arg1	methodology					402:412	Central composite design-based response surface methodology	354:412	Central composite design-based response surface methodology	354:412	Central composite design-based response surface methodology was used to approximate the combined effects of process parameters in simultaneous production of essential oils, pectin and a sugar-rich hydrolyzate.
34852446	2	39	theme	essential	511:519	arg1	oils					521:524	essential oils	511:524	essential oils	511:524	Central composite design-based response surface methodology was used to approximate the combined effects of process parameters in simultaneous production of essential oils, pectin and a sugar-rich hydrolyzate.
34852446	6	40	theme	biorefineries	1206:1218	arg1	study					1183:1187	the study	1179:1187	the study of biomass-based biorefineries	1179:1218	Design of experiments was efficient for process analysis and optimization providing a systems platform for the study of biomass-based biorefineries.
34852446	3	41	theme	production	658:667	arg1	efficiency					669:678	maximal production efficiency	650:678	maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading	650:738	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	6	42	theme	experiments	1082:1092	arg1	Design					1072:1077	Design	1072:1077	Design of experiments	1072:1092	Design of experiments was efficient for process analysis and optimization providing a systems platform for the study of biomass-based biorefineries.
34852446	2	43	theme	oils	521:524	arg1	pectin					527:532	pectin	527:532	pectin	527:532	Central composite design-based response surface methodology was used to approximate the combined effects of process parameters in simultaneous production of essential oils, pectin and a sugar-rich hydrolyzate.
34852446	2	43	theme	oils	521:524	arg1	production					497:506	simultaneous production	484:506	simultaneous production of essential oils	484:524	Central composite design-based response surface methodology was used to approximate the combined effects of process parameters in simultaneous production of essential oils, pectin and a sugar-rich hydrolyzate.
34852446	2	43	theme	oils	521:524	arg1	hydrolyzate					551:561	a sugar-rich hydrolyzate	538:561	a sugar-rich hydrolyzate	538:561	Central composite design-based response surface methodology was used to approximate the combined effects of process parameters in simultaneous production of essential oils, pectin and a sugar-rich hydrolyzate.
34852446	0	44	theme	waste	132:136	arg1	biorefinery					138:148	a citrus processing waste biorefinery	112:148	a citrus processing waste biorefinery	112:148	Ultrasound-assisted dilute acid hydrolysis for production of essential oils, pectin and bacterial cellulose via a citrus processing waste biorefinery.
34852446	3	45	theme	pectin	699:704	arg1	efficiency					669:678	maximal production efficiency	650:678	maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading	650:738	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	3	45	theme	pectin	699:704	arg1	concentration					752:764	1.21% acid concentration	741:764	1.21% acid concentration	741:764	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	3	45	theme	pectin	699:704	arg1	duration					779:786	34.2 min duration	770:786	34.2 min duration	770:786	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	4	46	theme	%	846:846	arg1	pectin					852:857	45% w/w pectin	844:857	45% w/w pectin	844:857	Maximum production yields of 0.12% w/w essential oils, 45% w/w pectin and 40% w/w sugars were achieved under optimized conditions in lab- and pilot-scale facilities.
34852446	2	47	theme	combined	442:449	arg1	effects					451:457	the combined effects	438:457	the combined effects of process parameters in simultaneous production of essential oils, pectin and a sugar-rich hydrolyzate	438:561	Central composite design-based response surface methodology was used to approximate the combined effects of process parameters in simultaneous production of essential oils, pectin and a sugar-rich hydrolyzate.
34852446	0	48	theme	essential	61:69	arg1	oils					71:74	essential oils	61:74	essential oils	61:74	Ultrasound-assisted dilute acid hydrolysis for production of essential oils, pectin and bacterial cellulose via a citrus processing waste biorefinery.
34852446	2	49	theme	design-based	372:383	arg1	methodology					402:412	Central composite design-based response surface methodology	354:412	Central composite design-based response surface methodology	354:412	Central composite design-based response surface methodology was used to approximate the combined effects of process parameters in simultaneous production of essential oils, pectin and a sugar-rich hydrolyzate.
34852446	1	50	theme	useful	334:339	arg1	commodities					341:351	useful commodities	334:351	useful commodities	334:351	An orange peel waste biorefinery was developed employing a design of experiments approach to optimize the ultrasound-assisted dilute acid hydrolysis process applied for production of useful commodities.
34852446	3	51	theme	solid	726:730	arg1	loading					732:738	5.75% solid loading	720:738	5.75% solid loading	720:738	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	3	52	theme	maximal	650:656	arg1	efficiency					669:678	maximal production efficiency	650:678	maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading	650:738	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	2	53	theme	composite	362:370	arg1	methodology					402:412	Central composite design-based response surface methodology	354:412	Central composite design-based response surface methodology	354:412	Central composite design-based response surface methodology was used to approximate the combined effects of process parameters in simultaneous production of essential oils, pectin and a sugar-rich hydrolyzate.
34852446	1	54	theme	commodities	341:351	arg1	production					320:329	production	320:329	production of useful commodities	320:351	An orange peel waste biorefinery was developed employing a design of experiments approach to optimize the ultrasound-assisted dilute acid hydrolysis process applied for production of useful commodities.
34852446	2	55	from	effects	451:457	arg1	pectin					527:532	pectin	527:532	pectin	527:532	Central composite design-based response surface methodology was used to approximate the combined effects of process parameters in simultaneous production of essential oils, pectin and a sugar-rich hydrolyzate.
34852446	2	55	from	effects	451:457	arg1	production					497:506	simultaneous production	484:506	simultaneous production of essential oils	484:524	Central composite design-based response surface methodology was used to approximate the combined effects of process parameters in simultaneous production of essential oils, pectin and a sugar-rich hydrolyzate.
34852446	2	55	from	effects	451:457	arg1	hydrolyzate					551:561	a sugar-rich hydrolyzate	538:561	a sugar-rich hydrolyzate	538:561	Central composite design-based response surface methodology was used to approximate the combined effects of process parameters in simultaneous production of essential oils, pectin and a sugar-rich hydrolyzate.
34852446	3	56	theme	oils	693:696	arg1	efficiency					669:678	maximal production efficiency	650:678	maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading	650:738	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	3	56	theme	oils	693:696	arg1	concentration					752:764	1.21% acid concentration	741:764	1.21% acid concentration	741:764	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	3	56	theme	oils	693:696	arg1	duration					779:786	34.2 min duration	770:786	34.2 min duration	770:786	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	2	57	theme	Central	354:360	arg1	methodology					402:412	Central composite design-based response surface methodology	354:412	Central composite design-based response surface methodology	354:412	Central composite design-based response surface methodology was used to approximate the combined effects of process parameters in simultaneous production of essential oils, pectin and a sugar-rich hydrolyzate.
34852446	1	58	theme	experiments	220:230	arg1	design					210:215	a design	208:215	a design of experiments	208:230	An orange peel waste biorefinery was developed employing a design of experiments approach to optimize the ultrasound-assisted dilute acid hydrolysis process applied for production of useful commodities.
34852446	3	59	theme	desirability	581:592	arg1	function					594:601	a desirability function	579:601	a desirability function	579:601	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	3	60	theme	5.75	720:723	arg1	%					724:724	%	724:724	%	724:724	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	4	61	theme	essential	828:836	arg1	oils					838:841	essential oils	828:841	0.12% w/w essential oils	818:841	Maximum production yields of 0.12% w/w essential oils, 45% w/w pectin and 40% w/w sugars were achieved under optimized conditions in lab- and pilot-scale facilities.
34852446	2	62	used	used	418:421	arg2	methodology					402:412	Central composite design-based response surface methodology	354:412	Central composite design-based response surface methodology	354:412	Central composite design-based response surface methodology was used to approximate the combined effects of process parameters in simultaneous production of essential oils, pectin and a sugar-rich hydrolyzate.
34852446	2	63	theme	sugar-rich	540:549	arg1	hydrolyzate					551:561	a sugar-rich hydrolyzate	538:561	a sugar-rich hydrolyzate	538:561	Central composite design-based response surface methodology was used to approximate the combined effects of process parameters in simultaneous production of essential oils, pectin and a sugar-rich hydrolyzate.
34852446	3	64	theme	%	724:724	arg1	loading					732:738	5.75% solid loading	720:738	5.75% solid loading	720:738	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	4	65	theme	45	844:845	arg1	%					846:846	%	846:846	%	846:846	Maximum production yields of 0.12% w/w essential oils, 45% w/w pectin and 40% w/w sugars were achieved under optimized conditions in lab- and pilot-scale facilities.
34852446	6	66	theme	systems	1158:1164	arg1	platform					1166:1173	a systems platform	1156:1173	a systems platform for the study of biomass-based biorefineries	1156:1218	Design of experiments was efficient for process analysis and optimization providing a systems platform for the study of biomass-based biorefineries.
34852446	0	67	theme	oils	71:74	arg1	production					47:56	production	47:56	production of essential oils	47:74	Ultrasound-assisted dilute acid hydrolysis for production of essential oils, pectin and bacterial cellulose via a citrus processing waste biorefinery.
34852446	3	68	theme	%	745:745	arg1	concentration					752:764	1.21% acid concentration	741:764	1.21% acid concentration	741:764	Application of a desirability function determined the optimal conditions required for maximal production efficiency of essential oils, pectin and sugars as 5.75% solid loading, 1.21% acid concentration and 34.2 min duration.
34852446	2	69	theme	parameters	470:479	arg1	effects					451:457	the combined effects	438:457	the combined effects of process parameters in simultaneous production of essential oils, pectin and a sugar-rich hydrolyzate	438:561	Central composite design-based response surface methodology was used to approximate the combined effects of process parameters in simultaneous production of essential oils, pectin and a sugar-rich hydrolyzate.
34852446	4	70	dep	%	822:822	arg1	oils					838:841	essential oils	828:841	0.12% w/w essential oils	818:841	Maximum production yields of 0.12% w/w essential oils, 45% w/w pectin and 40% w/w sugars were achieved under optimized conditions in lab- and pilot-scale facilities.
34646811	4	0	theme	urine	872:876	arg1	constituents					816:827	the N-glycan constituents	803:827	the N-glycan constituents of EPS urine samples, prostatic fluids and urine using imaging mass spectrometry for detection	803:922	In this report, an approach is described to directly profile the N-glycan constituents of EPS urine samples, prostatic fluids and urine using imaging mass spectrometry for detection.
34646811	7	1	from	tissues	1336:1342	arg1	N-glycans					1305:1313	tissue N-glycans	1298:1313	tissue N-glycans from prostate cancer tissues	1298:1342	A comparison of the N-glycans detected from the fluids with tissue N-glycans from prostate cancer tissues was done, indicating a subset of N-glycans present in fluids derived from the gland lumens.
34646811	3	2	theme	processing	689:698	arg1	requirements					700:711	minimal sample processing requirements	674:711	minimal sample processing requirements	674:711	Methods to characterize these N-glycan constituents directly from clinical samples in a timely manner and with minimal sample processing requirements are not currently available.
34646811	4	3	theme	imaging	884:890	arg1	spectrometry					897:908	imaging mass spectrometry	884:908	imaging mass spectrometry for detection	884:922	In this report, an approach is described to directly profile the N-glycan constituents of EPS urine samples, prostatic fluids and urine using imaging mass spectrometry for detection.
34646811	7	4	theme	gland	1422:1426	arg1	lumens					1428:1433	the gland lumens	1418:1433	the gland lumens	1418:1433	A comparison of the N-glycans detected from the fluids with tissue N-glycans from prostate cancer tissues was done, indicating a subset of N-glycans present in fluids derived from the gland lumens.
34646811	1	5	theme	diagnostic	248:257	arg1	assays					274:279	diagnostic and prognostic assays	248:279	diagnostic and prognostic assays for prostate cancer	248:299	Expressed prostatic secretions (EPS), also called post digital rectal exam urines, are proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer.
34646811	6	6	theme	EPS	1169:1171	arg1	vesicles					1228:1235	urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles	1156:1235	urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles	1156:1235	Over 100 N-glycan compositions can be detected with this method, and it works with urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles.
34646811	1	7	theme	exam	174:177	arg1	urines					179:184	digital rectal exam urines	159:184	digital rectal exam urines	159:184	Expressed prostatic secretions (EPS), also called post digital rectal exam urines, are proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer.
34646811	4	8	theme	fluids	861:866	arg1	constituents					816:827	the N-glycan constituents	803:827	the N-glycan constituents of EPS urine samples, prostatic fluids and urine using imaging mass spectrometry for detection	803:922	In this report, an approach is described to directly profile the N-glycan constituents of EPS urine samples, prostatic fluids and urine using imaging mass spectrometry for detection.
34646811	7	9	attach	derived	1405:1411	arg1	lumens					1428:1433	the gland lumens	1418:1433	the gland lumens	1418:1433	A comparison of the N-glycans detected from the fluids with tissue N-glycans from prostate cancer tissues was done, indicating a subset of N-glycans present in fluids derived from the gland lumens.
34646811	7	9	attach	derived	1405:1411	arg2	fluids					1398:1403	fluids	1398:1403	fluids derived from the gland lumens	1398:1433	A comparison of the N-glycans detected from the fluids with tissue N-glycans from prostate cancer tissues was done, indicating a subset of N-glycans present in fluids derived from the gland lumens.
34646811	2	10	theme	biomarker	541:549	arg1	candidates					551:560	potential new biomarker candidates	527:560	potential new biomarker candidates	527:560	These fluids contain an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland, and the ability to detect changes in their N-glycans composition as a reflection of disease state represents potential new biomarker candidates.
34646811	7	11	theme	present	1387:1393	arg1	N-glycans					1377:1385	N-glycans	1377:1385	N-glycans present in fluids derived from the gland lumens	1377:1433	A comparison of the N-glycans detected from the fluids with tissue N-glycans from prostate cancer tissues was done, indicating a subset of N-glycans present in fluids derived from the gland lumens.
34646811	1	12	theme	proximal	191:198	arg1	fluids					200:205	proximal fluids	191:205	proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer	191:299	Expressed prostatic secretions (EPS), also called post digital rectal exam urines, are proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer.
34646811	1	12	theme	proximal	191:198	arg1	secretions					124:133	Expressed prostatic secretions	104:133	Expressed prostatic secretions (EPS)	104:139	Expressed prostatic secretions (EPS), also called post digital rectal exam urines, are proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer.
34646811	2	13	theme	potential	527:535	arg1	candidates					551:560	potential new biomarker candidates	527:560	potential new biomarker candidates	527:560	These fluids contain an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland, and the ability to detect changes in their N-glycans composition as a reflection of disease state represents potential new biomarker candidates.
34646811	6	14	theme	Over	1073:1076	arg1	compositions					1091:1102	Over 100 N-glycan compositions	1073:1102	Over 100 N-glycan compositions	1073:1102	Over 100 N-glycan compositions can be detected with this method, and it works with urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles.
34646811	8	15	theme	developed	1440:1448	arg1	amenable					1472:1479	amenable	1472:1479	amenable	1472:1479	The developed N-glycan profiling is amenable to analysis of larger clinical cohorts and adaptable to other biofluids.
34646811	8	15	theme	developed	1440:1448	arg1	profiling					1459:1467	The developed N-glycan profiling	1436:1467	The developed N-glycan profiling	1436:1467	The developed N-glycan profiling is amenable to analysis of larger clinical cohorts and adaptable to other biofluids.
34646811	6	16	theme	extracellular	1214:1226	arg1	vesicles					1228:1235	urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles	1156:1235	urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles	1156:1235	Over 100 N-glycan compositions can be detected with this method, and it works with urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles.
34646811	5	17	theme	peptide	1040:1046	arg1	digestion					1062:1070	peptide N-glycosidase digestion	1040:1070	peptide N-glycosidase digestion	1040:1070	An amine reactive slide is used to immobilize glycoproteins from a few microliters of spotted samples, followed by peptide N-glycosidase digestion.
34646811	7	18	from	present	1387:1393	arg1	fluids					1398:1403	fluids	1398:1403	fluids derived from the gland lumens	1398:1433	A comparison of the N-glycans detected from the fluids with tissue N-glycans from prostate cancer tissues was done, indicating a subset of N-glycans present in fluids derived from the gland lumens.
34646811	6	19	theme	urine	1196:1200	arg1	vesicles					1228:1235	urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles	1156:1235	urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles	1156:1235	Over 100 N-glycan compositions can be detected with this method, and it works with urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles.
34646811	6	20	theme	prostatic	1174:1182	arg1	fluids					1184:1189	prostatic fluids	1174:1189	prostatic fluids	1174:1189	Over 100 N-glycan compositions can be detected with this method, and it works with urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles.
34646811	5	21	theme	reactive	934:941	arg1	slide					943:947	An amine reactive slide	925:947	An amine reactive slide	925:947	An amine reactive slide is used to immobilize glycoproteins from a few microliters of spotted samples, followed by peptide N-glycosidase digestion.
34646811	7	22	from	fluids	1398:1403	arg1	present					1387:1393	present	1387:1393	present	1387:1393	A comparison of the N-glycans detected from the fluids with tissue N-glycans from prostate cancer tissues was done, indicating a subset of N-glycans present in fluids derived from the gland lumens.
34646811	5	23	gly	glycoproteins	971:983	arg1	glycoproteins					971:983	glycoproteins	971:983	glycoproteins	971:983	An amine reactive slide is used to immobilize glycoproteins from a few microliters of spotted samples, followed by peptide N-glycosidase digestion.
34646811	0	24	theme	Glycoproteins	62:74	arg1	Profiling					23:31	Direct N-Glycosylation Profiling	0:31	Direct N-Glycosylation Profiling of Urine and Prostatic Fluid Glycoproteins and Extracellular Vesicles	0:101	Direct N-Glycosylation Profiling of Urine and Prostatic Fluid Glycoproteins and Extracellular Vesicles.
34646811	0	25	theme	Extracellular	80:92	arg1	Vesicles					94:101	Extracellular Vesicles	80:101	Extracellular Vesicles	80:101	Direct N-Glycosylation Profiling of Urine and Prostatic Fluid Glycoproteins and Extracellular Vesicles.
34646811	1	26	theme	digital	159:165	arg1	urines					179:184	digital rectal exam urines	159:184	digital rectal exam urines	159:184	Expressed prostatic secretions (EPS), also called post digital rectal exam urines, are proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer.
34646811	2	27	theme	state	510:514	arg1	changes					444:450	changes	444:450	changes in their N-glycans composition	444:481	These fluids contain an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland, and the ability to detect changes in their N-glycans composition as a reflection of disease state represents potential new biomarker candidates.
34646811	2	27	theme	state	510:514	arg1	reflection					488:497	a reflection	486:497	a reflection of disease state	486:514	These fluids contain an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland, and the ability to detect changes in their N-glycans composition as a reflection of disease state represents potential new biomarker candidates.
34646811	8	28	theme	larger	1496:1501	arg1	cohorts					1512:1518	larger clinical cohorts	1496:1518	larger clinical cohorts	1496:1518	The developed N-glycan profiling is amenable to analysis of larger clinical cohorts and adaptable to other biofluids.
34646811	8	29	theme	cohorts	1512:1518	arg1	analysis					1484:1491	analysis	1484:1491	analysis of larger clinical cohorts	1484:1518	The developed N-glycan profiling is amenable to analysis of larger clinical cohorts and adaptable to other biofluids.
34646811	3	30	from	samples	638:644	arg1	manner					658:663	a timely manner	649:663	a timely manner	649:663	Methods to characterize these N-glycan constituents directly from clinical samples in a timely manner and with minimal sample processing requirements are not currently available.
34646811	2	31	theme	vesicles	377:384	arg1	number					335:340	an abundant number	323:340	an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland	323:415	These fluids contain an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland, and the ability to detect changes in their N-glycans composition as a reflection of disease state represents potential new biomarker candidates.
34646811	0	32	theme	Direct	0:5	arg1	Profiling					23:31	Direct N-Glycosylation Profiling	0:31	Direct N-Glycosylation Profiling of Urine and Prostatic Fluid Glycoproteins and Extracellular Vesicles	0:101	Direct N-Glycosylation Profiling of Urine and Prostatic Fluid Glycoproteins and Extracellular Vesicles.
34646811	1	33	theme	Expressed	104:112	arg1	fluids					200:205	proximal fluids	191:205	proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer	191:299	Expressed prostatic secretions (EPS), also called post digital rectal exam urines, are proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer.
34646811	1	33	theme	Expressed	104:112	arg1	EPS					136:138	EPS	136:138	EPS	136:138	Expressed prostatic secretions (EPS), also called post digital rectal exam urines, are proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer.
34646811	1	33	theme	Expressed	104:112	arg1	secretions					124:133	Expressed prostatic secretions	104:133	Expressed prostatic secretions (EPS)	104:139	Expressed prostatic secretions (EPS), also called post digital rectal exam urines, are proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer.
34646811	7	34	theme	prostate	1320:1327	arg1	tissues					1336:1342	prostate cancer tissues	1320:1342	prostate cancer tissues	1320:1342	A comparison of the N-glycans detected from the fluids with tissue N-glycans from prostate cancer tissues was done, indicating a subset of N-glycans present in fluids derived from the gland lumens.
34646811	8	35	theme	other	1537:1541	arg1	biofluids					1543:1551	other biofluids	1537:1551	other biofluids	1537:1551	The developed N-glycan profiling is amenable to analysis of larger clinical cohorts and adaptable to other biofluids.
34646811	2	36	gly	glycoproteins	345:357	arg1	glycoproteins					345:357	glycoproteins	345:357	glycoproteins	345:357	These fluids contain an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland, and the ability to detect changes in their N-glycans composition as a reflection of disease state represents potential new biomarker candidates.
34646811	2	37	theme	prostate	402:409	arg1	gland					411:415	the prostate gland	398:415	the prostate gland	398:415	These fluids contain an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland, and the ability to detect changes in their N-glycans composition as a reflection of disease state represents potential new biomarker candidates.
34646811	0	38	theme	Urine	36:40	arg1	Glycoproteins					62:74	Urine and Prostatic Fluid Glycoproteins	36:74	Urine and Prostatic Fluid Glycoproteins	36:74	Direct N-Glycosylation Profiling of Urine and Prostatic Fluid Glycoproteins and Extracellular Vesicles.
34646811	1	39	theme	prostate	285:292	arg1	cancer					294:299	prostate cancer	285:299	prostate cancer	285:299	Expressed prostatic secretions (EPS), also called post digital rectal exam urines, are proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer.
34646811	1	40	used	used	239:242	arg2	secretions					124:133	Expressed prostatic secretions	104:133	Expressed prostatic secretions (EPS)	104:139	Expressed prostatic secretions (EPS), also called post digital rectal exam urines, are proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer.
34646811	1	40	used	used	239:242	arg2	fluids					200:205	proximal fluids	191:205	proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer	191:299	Expressed prostatic secretions (EPS), also called post digital rectal exam urines, are proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer.
34646811	4	41	theme	urine	836:840	arg1	samples					842:848	EPS urine samples	832:848	EPS urine samples	832:848	In this report, an approach is described to directly profile the N-glycan constituents of EPS urine samples, prostatic fluids and urine using imaging mass spectrometry for detection.
34646811	2	42	theme	abundant	326:333	arg1	number					335:340	an abundant number	323:340	an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland	323:415	These fluids contain an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland, and the ability to detect changes in their N-glycans composition as a reflection of disease state represents potential new biomarker candidates.
34646811	0	43	theme	Prostatic	46:54	arg1	Fluid					56:60	Prostatic Fluid	46:60	Prostatic Fluid	46:60	Direct N-Glycosylation Profiling of Urine and Prostatic Fluid Glycoproteins and Extracellular Vesicles.
34646811	3	44	theme	sample	682:687	arg1	requirements					700:711	minimal sample processing requirements	674:711	minimal sample processing requirements	674:711	Methods to characterize these N-glycan constituents directly from clinical samples in a timely manner and with minimal sample processing requirements are not currently available.
34646811	4	45	theme	N-glycan	807:814	arg1	constituents					816:827	the N-glycan constituents	803:827	the N-glycan constituents of EPS urine samples, prostatic fluids and urine using imaging mass spectrometry for detection	803:922	In this report, an approach is described to directly profile the N-glycan constituents of EPS urine samples, prostatic fluids and urine using imaging mass spectrometry for detection.
34646811	5	46	theme	spotted	1011:1017	arg1	samples					1019:1025	spotted samples	1011:1025	spotted samples	1011:1025	An amine reactive slide is used to immobilize glycoproteins from a few microliters of spotted samples, followed by peptide N-glycosidase digestion.
34646811	1	47	theme	rectal	167:172	arg1	urines					179:184	digital rectal exam urines	159:184	digital rectal exam urines	159:184	Expressed prostatic secretions (EPS), also called post digital rectal exam urines, are proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer.
34646811	4	48	theme	samples	842:848	arg1	constituents					816:827	the N-glycan constituents	803:827	the N-glycan constituents of EPS urine samples, prostatic fluids and urine using imaging mass spectrometry for detection	803:922	In this report, an approach is described to directly profile the N-glycan constituents of EPS urine samples, prostatic fluids and urine using imaging mass spectrometry for detection.
34646811	6	49	theme	urine	1163:1167	arg1	EPS					1169:1171	urine EPS	1163:1171	urine EPS	1163:1171	Over 100 N-glycan compositions can be detected with this method, and it works with urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles.
34646811	3	50	with	samples	638:644	arg1	requirements					700:711	minimal sample processing requirements	674:711	minimal sample processing requirements	674:711	Methods to characterize these N-glycan constituents directly from clinical samples in a timely manner and with minimal sample processing requirements are not currently available.
34646811	4	51	theme	prostatic	851:859	arg1	fluids					861:866	prostatic fluids	851:866	prostatic fluids	851:866	In this report, an approach is described to directly profile the N-glycan constituents of EPS urine samples, prostatic fluids and urine using imaging mass spectrometry for detection.
34646811	6	52	theme	urine	1156:1160	arg1	vesicles					1228:1235	urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles	1156:1235	urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles	1156:1235	Over 100 N-glycan compositions can be detected with this method, and it works with urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles.
34646811	6	53	link	EPS-derived	1202:1212	arg1	vesicles					1228:1235	urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles	1156:1235	urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles	1156:1235	Over 100 N-glycan compositions can be detected with this method, and it works with urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles.
34646811	7	54	with	fluids	1286:1291	arg1	N-glycans					1305:1313	tissue N-glycans	1298:1313	tissue N-glycans from prostate cancer tissues	1298:1342	A comparison of the N-glycans detected from the fluids with tissue N-glycans from prostate cancer tissues was done, indicating a subset of N-glycans present in fluids derived from the gland lumens.
34646811	2	55	theme	new	537:539	arg1	candidates					551:560	potential new biomarker candidates	527:560	potential new biomarker candidates	527:560	These fluids contain an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland, and the ability to detect changes in their N-glycans composition as a reflection of disease state represents potential new biomarker candidates.
34646811	7	56	attach	present	1387:1393	arg2	N-glycans					1377:1385	N-glycans	1377:1385	N-glycans present in fluids derived from the gland lumens	1377:1433	A comparison of the N-glycans detected from the fluids with tissue N-glycans from prostate cancer tissues was done, indicating a subset of N-glycans present in fluids derived from the gland lumens.
34646811	7	56	attach	present	1387:1393	arg1	fluids					1398:1403	fluids	1398:1403	fluids derived from the gland lumens	1398:1433	A comparison of the N-glycans detected from the fluids with tissue N-glycans from prostate cancer tissues was done, indicating a subset of N-glycans present in fluids derived from the gland lumens.
34646811	3	57	theme	N-glycan	593:600	arg1	constituents					602:613	these N-glycan constituents	587:613	these N-glycan constituents	587:613	Methods to characterize these N-glycan constituents directly from clinical samples in a timely manner and with minimal sample processing requirements are not currently available.
34646811	8	58	theme	N-glycan	1450:1457	arg1	amenable					1472:1479	amenable	1472:1479	amenable	1472:1479	The developed N-glycan profiling is amenable to analysis of larger clinical cohorts and adaptable to other biofluids.
34646811	8	58	theme	N-glycan	1450:1457	arg1	profiling					1459:1467	The developed N-glycan profiling	1436:1467	The developed N-glycan profiling	1436:1467	The developed N-glycan profiling is amenable to analysis of larger clinical cohorts and adaptable to other biofluids.
34646811	7	59	theme	N-glycans	1377:1385	arg1	subset					1367:1372	a subset	1365:1372	a subset of N-glycans present in fluids derived from the gland lumens	1365:1433	A comparison of the N-glycans detected from the fluids with tissue N-glycans from prostate cancer tissues was done, indicating a subset of N-glycans present in fluids derived from the gland lumens.
34646811	4	60	theme	mass	892:895	arg1	spectrometry					897:908	imaging mass spectrometry	884:908	imaging mass spectrometry for detection	884:922	In this report, an approach is described to directly profile the N-glycan constituents of EPS urine samples, prostatic fluids and urine using imaging mass spectrometry for detection.
34646811	6	61	theme	EPS-derived	1202:1212	arg1	vesicles					1228:1235	urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles	1156:1235	urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles	1156:1235	Over 100 N-glycan compositions can be detected with this method, and it works with urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles.
34646811	5	62	theme	N-glycosidase	1048:1060	arg1	digestion					1062:1070	peptide N-glycosidase digestion	1040:1070	peptide N-glycosidase digestion	1040:1070	An amine reactive slide is used to immobilize glycoproteins from a few microliters of spotted samples, followed by peptide N-glycosidase digestion.
34646811	3	63	theme	clinical	629:636	arg1	samples					638:644	clinical samples	629:644	clinical samples in a timely manner and with minimal sample processing requirements	629:711	Methods to characterize these N-glycan constituents directly from clinical samples in a timely manner and with minimal sample processing requirements are not currently available.
34646811	5	64	theme	amine	928:932	arg1	slide					943:947	An amine reactive slide	925:947	An amine reactive slide	925:947	An amine reactive slide is used to immobilize glycoproteins from a few microliters of spotted samples, followed by peptide N-glycosidase digestion.
34646811	6	65	theme	fluids	1184:1189	arg1	vesicles					1228:1235	urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles	1156:1235	urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles	1156:1235	Over 100 N-glycan compositions can be detected with this method, and it works with urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles.
34646811	0	66	theme	Vesicles	94:101	arg1	Profiling					23:31	Direct N-Glycosylation Profiling	0:31	Direct N-Glycosylation Profiling of Urine and Prostatic Fluid Glycoproteins and Extracellular Vesicles	0:101	Direct N-Glycosylation Profiling of Urine and Prostatic Fluid Glycoproteins and Extracellular Vesicles.
34646811	2	67	contain	contain	315:321	arg1	fluids					308:313	These fluids	302:313	These fluids	302:313	These fluids contain an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland, and the ability to detect changes in their N-glycans composition as a reflection of disease state represents potential new biomarker candidates.
34646811	2	67	contain	contain	315:321	arg2	number					335:340	an abundant number	323:340	an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland	323:415	These fluids contain an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland, and the ability to detect changes in their N-glycans composition as a reflection of disease state represents potential new biomarker candidates.
34646811	3	68	dep	Methods	563:569	arg1	characterize					574:585	characterize	574:585	to characterize these N-glycan constituents directly from clinical samples in a timely manner and with minimal sample processing requirements	571:711	Methods to characterize these N-glycan constituents directly from clinical samples in a timely manner and with minimal sample processing requirements are not currently available.
34646811	2	69	theme	N-glycans	461:469	arg1	composition					471:481	their N-glycans composition	455:481	their N-glycans composition	455:481	These fluids contain an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland, and the ability to detect changes in their N-glycans composition as a reflection of disease state represents potential new biomarker candidates.
34646811	8	70	theme	clinical	1503:1510	arg1	cohorts					1512:1518	larger clinical cohorts	1496:1518	larger clinical cohorts	1496:1518	The developed N-glycan profiling is amenable to analysis of larger clinical cohorts and adaptable to other biofluids.
34646811	2	71	theme	disease	502:508	arg1	state					510:514	disease state	502:514	disease state	502:514	These fluids contain an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland, and the ability to detect changes in their N-glycans composition as a reflection of disease state represents potential new biomarker candidates.
34646811	2	72	from	changes	444:450	arg1	composition					471:481	their N-glycans composition	455:481	their N-glycans composition	455:481	These fluids contain an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland, and the ability to detect changes in their N-glycans composition as a reflection of disease state represents potential new biomarker candidates.
34646811	7	73	theme	cancer	1329:1334	arg1	tissues					1336:1342	prostate cancer tissues	1320:1342	prostate cancer tissues	1320:1342	A comparison of the N-glycans detected from the fluids with tissue N-glycans from prostate cancer tissues was done, indicating a subset of N-glycans present in fluids derived from the gland lumens.
34646811	5	74	used	used	952:955	arg2	slide					943:947	An amine reactive slide	925:947	An amine reactive slide	925:947	An amine reactive slide is used to immobilize glycoproteins from a few microliters of spotted samples, followed by peptide N-glycosidase digestion.
34646811	0	75	theme	N-Glycosylation	7:21	arg1	Profiling					23:31	Direct N-Glycosylation Profiling	0:31	Direct N-Glycosylation Profiling of Urine and Prostatic Fluid Glycoproteins and Extracellular Vesicles	0:101	Direct N-Glycosylation Profiling of Urine and Prostatic Fluid Glycoproteins and Extracellular Vesicles.
34646811	6	76	theme	100 N-glycan	1078:1089	arg1	compositions					1091:1102	Over 100 N-glycan compositions	1073:1102	Over 100 N-glycan compositions	1073:1102	Over 100 N-glycan compositions can be detected with this method, and it works with urine, urine EPS, prostatic fluids, and urine EPS-derived extracellular vesicles.
34646811	2	77	theme	extracellular	363:375	arg1	vesicles					377:384	extracellular vesicles	363:384	extracellular vesicles	363:384	These fluids contain an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland, and the ability to detect changes in their N-glycans composition as a reflection of disease state represents potential new biomarker candidates.
34646811	1	78	theme	prognostic	263:272	arg1	assays					274:279	diagnostic and prognostic assays	248:279	diagnostic and prognostic assays for prostate cancer	248:299	Expressed prostatic secretions (EPS), also called post digital rectal exam urines, are proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer.
34646811	1	79	theme	prostatic	114:122	arg1	fluids					200:205	proximal fluids	191:205	proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer	191:299	Expressed prostatic secretions (EPS), also called post digital rectal exam urines, are proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer.
34646811	1	79	theme	prostatic	114:122	arg1	EPS					136:138	EPS	136:138	EPS	136:138	Expressed prostatic secretions (EPS), also called post digital rectal exam urines, are proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer.
34646811	1	79	theme	prostatic	114:122	arg1	secretions					124:133	Expressed prostatic secretions	104:133	Expressed prostatic secretions (EPS)	104:139	Expressed prostatic secretions (EPS), also called post digital rectal exam urines, are proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer.
34646811	7	80	theme	tissue	1298:1303	arg1	N-glycans					1305:1313	tissue N-glycans	1298:1313	tissue N-glycans from prostate cancer tissues	1298:1342	A comparison of the N-glycans detected from the fluids with tissue N-glycans from prostate cancer tissues was done, indicating a subset of N-glycans present in fluids derived from the gland lumens.
34646811	3	81	theme	timely	651:656	arg1	manner					658:663	a timely manner	649:663	a timely manner	649:663	Methods to characterize these N-glycan constituents directly from clinical samples in a timely manner and with minimal sample processing requirements are not currently available.
34646811	5	82	theme	samples	1019:1025	arg1	microliters					996:1006	a few microliters	990:1006	a few microliters of spotted samples	990:1025	An amine reactive slide is used to immobilize glycoproteins from a few microliters of spotted samples, followed by peptide N-glycosidase digestion.
34646811	0	83	theme	Fluid	56:60	arg1	Glycoproteins					62:74	Urine and Prostatic Fluid Glycoproteins	36:74	Urine and Prostatic Fluid Glycoproteins	36:74	Direct N-Glycosylation Profiling of Urine and Prostatic Fluid Glycoproteins and Extracellular Vesicles.
34646811	3	84	theme	minimal	674:680	arg1	requirements					700:711	minimal sample processing requirements	674:711	minimal sample processing requirements	674:711	Methods to characterize these N-glycan constituents directly from clinical samples in a timely manner and with minimal sample processing requirements are not currently available.
34646811	4	85	theme	EPS	832:834	arg1	samples					842:848	EPS urine samples	832:848	EPS urine samples	832:848	In this report, an approach is described to directly profile the N-glycan constituents of EPS urine samples, prostatic fluids and urine using imaging mass spectrometry for detection.
34646811	5	86	theme	few	992:994	arg1	microliters					996:1006	a few microliters	990:1006	a few microliters of spotted samples	990:1025	An amine reactive slide is used to immobilize glycoproteins from a few microliters of spotted samples, followed by peptide N-glycosidase digestion.
34646811	7	87	theme	N-glycans	1258:1266	arg1	comparison					1240:1249	A comparison	1238:1249	A comparison of the N-glycans detected from the fluids with tissue N-glycans from prostate cancer tissues	1238:1342	A comparison of the N-glycans detected from the fluids with tissue N-glycans from prostate cancer tissues was done, indicating a subset of N-glycans present in fluids derived from the gland lumens.
34646811	1	88	theme	prostate	214:221	arg1	fluids					200:205	proximal fluids	191:205	proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer	191:299	Expressed prostatic secretions (EPS), also called post digital rectal exam urines, are proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer.
34646811	1	88	theme	prostate	214:221	arg1	secretions					124:133	Expressed prostatic secretions	104:133	Expressed prostatic secretions (EPS)	104:139	Expressed prostatic secretions (EPS), also called post digital rectal exam urines, are proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer.
34646811	2	89	theme	glycoproteins	345:357	arg1	number					335:340	an abundant number	323:340	an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland	323:415	These fluids contain an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland, and the ability to detect changes in their N-glycans composition as a reflection of disease state represents potential new biomarker candidates.
32771505	5	0	theme	Increasing	769:778	arg1	concentration					780:792	Increasing concentration	769:792	Increasing concentration of the pectin	769:806	Increasing concentration of the pectin promoted increasing gel strength of the composite microparticles.
32771505	4	1	theme	AU	758:759	arg1	pectin					761:766	the AU pectin	754:766	the AU pectin	754:766	Decreasing degree of methyl esterification (DM) of the SVC pectin promoted Ca2+ binding in comparison with the AU pectin.
32771505	4	2	theme	pectin	706:711	arg1	DM					691:692	DM	691:692	DM	691:692	Decreasing degree of methyl esterification (DM) of the SVC pectin promoted Ca2+ binding in comparison with the AU pectin.
32771505	4	2	theme	pectin	706:711	arg1	esterification					675:688	methyl esterification	668:688	methyl esterification (DM) of the SVC pectin	668:711	Decreasing degree of methyl esterification (DM) of the SVC pectin promoted Ca2+ binding in comparison with the AU pectin.
32771505	7	3	contain	had	1114:1116	arg2	degree					1135:1140	a lower swelling degree	1118:1140	a lower swelling degree in the simulated digestive fluids	1118:1174	The microparticle gel formulations with a higher pectin concentration had a lower swelling degree in the simulated digestive fluids.
32771505	7	3	contain	had	1114:1116	arg1	formulations					1066:1077	The microparticle gel formulations	1044:1077	The microparticle gel formulations with a higher pectin concentration	1044:1112	The microparticle gel formulations with a higher pectin concentration had a lower swelling degree in the simulated digestive fluids.
32771505	7	4	with	formulations	1066:1077	arg1	concentration					1100:1112	a higher pectin concentration	1084:1112	a higher pectin concentration	1084:1112	The microparticle gel formulations with a higher pectin concentration had a lower swelling degree in the simulated digestive fluids.
32771505	7	5	theme	swelling	1126:1133	arg1	degree					1135:1140	a lower swelling degree	1118:1140	a lower swelling degree in the simulated digestive fluids	1118:1174	The microparticle gel formulations with a higher pectin concentration had a lower swelling degree in the simulated digestive fluids.
32771505	8	6	theme	gel	1216:1218	arg1	formulations					1220:1231	the gel formulations	1212:1231	the gel formulations	1212:1231	The addition of the carrageenan to the gel formulations led to an increase in the swelling degree in comparison with that without carrageenan.
32771505	1	7	theme	composite	152:160	arg1	microparticles					166:179	composite gel microparticles	152:179	composite gel microparticles based on the pectin (campion callus culture (SVC) and commercial apple (AU) pectins) and κ-carrageenan	152:282	The aim of this research was to produce composite gel microparticles based on the pectin (campion callus culture (SVC) and commercial apple (AU) pectins) and κ-carrageenan and investigate the relationship between the characteristics and swelling properties of the composite microparticles.
32771505	6	8	theme	higher	993:998	arg1	linearity					1000:1008	a higher linearity	991:1008	a higher linearity	991:1008	The higher gel strength of the composite microparticles based on the SVC pectin was probably due to the lower DM and a higher linearity in comparison with the AU pectin.
32771505	7	9	theme	digestive	1159:1167	arg1	fluids					1169:1174	the simulated digestive fluids	1145:1174	the simulated digestive fluids	1145:1174	The microparticle gel formulations with a higher pectin concentration had a lower swelling degree in the simulated digestive fluids.
32771505	5	10	theme	pectin	801:806	arg1	concentration					780:792	Increasing concentration	769:792	Increasing concentration of the pectin	769:806	Increasing concentration of the pectin promoted increasing gel strength of the composite microparticles.
32771505	1	11	theme	commercial	235:244	arg1	pectins					257:263	commercial apple (AU) pectins	235:263	commercial apple (AU) pectins	235:263	The aim of this research was to produce composite gel microparticles based on the pectin (campion callus culture (SVC) and commercial apple (AU) pectins) and κ-carrageenan and investigate the relationship between the characteristics and swelling properties of the composite microparticles.
32771505	6	12	theme	lower	978:982	arg1	strength					889:896	The higher gel strength	874:896	The higher gel strength of the composite microparticles based on the SVC pectin	874:952	The higher gel strength of the composite microparticles based on the SVC pectin was probably due to the lower DM and a higher linearity in comparison with the AU pectin.
32771505	6	12	theme	lower	978:982	arg1	DM					984:985	the lower DM	974:985	the lower DM	974:985	The higher gel strength of the composite microparticles based on the SVC pectin was probably due to the lower DM and a higher linearity in comparison with the AU pectin.
32771505	6	13	theme	microparticles	915:928	arg1	DM					984:985	the lower DM	974:985	the lower DM	974:985	The higher gel strength of the composite microparticles based on the SVC pectin was probably due to the lower DM and a higher linearity in comparison with the AU pectin.
32771505	6	13	theme	microparticles	915:928	arg1	strength					889:896	The higher gel strength	874:896	The higher gel strength of the composite microparticles based on the SVC pectin	874:952	The higher gel strength of the composite microparticles based on the SVC pectin was probably due to the lower DM and a higher linearity in comparison with the AU pectin.
32771505	1	14	theme	apple	246:250	arg1	pectins					257:263	commercial apple (AU) pectins	235:263	commercial apple (AU) pectins	235:263	The aim of this research was to produce composite gel microparticles based on the pectin (campion callus culture (SVC) and commercial apple (AU) pectins) and κ-carrageenan and investigate the relationship between the characteristics and swelling properties of the composite microparticles.
32771505	4	15	theme	SVC	702:704	arg1	pectin					706:711	the SVC pectin	698:711	the SVC pectin	698:711	Decreasing degree of methyl esterification (DM) of the SVC pectin promoted Ca2+ binding in comparison with the AU pectin.
32771505	6	16	theme	composite	905:913	arg1	microparticles					915:928	the composite microparticles	901:928	the composite microparticles based on the SVC pectin	901:952	The higher gel strength of the composite microparticles based on the SVC pectin was probably due to the lower DM and a higher linearity in comparison with the AU pectin.
32771505	5	17	theme	composite	848:856	arg1	microparticles					858:871	the composite microparticles	844:871	the composite microparticles	844:871	Increasing concentration of the pectin promoted increasing gel strength of the composite microparticles.
32771505	5	18	theme	increasing	817:826	arg1	strength					832:839	increasing gel strength	817:839	increasing gel strength of the composite microparticles	817:871	Increasing concentration of the pectin promoted increasing gel strength of the composite microparticles.
32771505	9	19	theme	decreasing	1440:1449	arg1	degree					1460:1465	decreasing swelling degree	1440:1465	decreasing swelling degree of the composite microparticles	1440:1497	The correlation analysis indicated that increasing initial Ca2+ content and gel strength of the microparticles promoted decreasing swelling degree of the composite microparticles.
32771505	1	20	theme	gel	162:164	arg1	microparticles					166:179	composite gel microparticles	152:179	composite gel microparticles based on the pectin (campion callus culture (SVC) and commercial apple (AU) pectins) and κ-carrageenan	152:282	The aim of this research was to produce composite gel microparticles based on the pectin (campion callus culture (SVC) and commercial apple (AU) pectins) and κ-carrageenan and investigate the relationship between the characteristics and swelling properties of the composite microparticles.
32771505	2	21	theme	dehydration	450:460	arg1	techniques					462:471	emulsion dehydration techniques	441:471	emulsion dehydration techniques	441:471	The microparticles were obtained using emulsion dehydration techniques with successive incubation in calcium chloride solution.
32771505	2	22	theme	successive	478:487	arg1	incubation					489:498	successive incubation	478:498	successive incubation in calcium chloride solution	478:527	The microparticles were obtained using emulsion dehydration techniques with successive incubation in calcium chloride solution.
32771505	9	23	theme	gel	1396:1398	arg1	strength					1400:1407	gel strength	1396:1407	gel strength	1396:1407	The correlation analysis indicated that increasing initial Ca2+ content and gel strength of the microparticles promoted decreasing swelling degree of the composite microparticles.
32771505	4	24	theme	esterification	675:688	arg1	degree					658:663	Decreasing degree	647:663	Decreasing degree of methyl esterification (DM) of the SVC pectin	647:711	Decreasing degree of methyl esterification (DM) of the SVC pectin promoted Ca2+ binding in comparison with the AU pectin.
32771505	2	25	theme	emulsion	441:448	arg1	techniques					462:471	emulsion dehydration techniques	441:471	emulsion dehydration techniques	441:471	The microparticles were obtained using emulsion dehydration techniques with successive incubation in calcium chloride solution.
32771505	1	26	theme	swelling	349:356	arg1	properties					358:367	swelling properties	349:367	swelling properties	349:367	The aim of this research was to produce composite gel microparticles based on the pectin (campion callus culture (SVC) and commercial apple (AU) pectins) and κ-carrageenan and investigate the relationship between the characteristics and swelling properties of the composite microparticles.
32771505	6	27	from	linearity	1000:1008	arg1	comparison					1013:1022	comparison	1013:1022	comparison with the AU pectin	1013:1041	The higher gel strength of the composite microparticles based on the SVC pectin was probably due to the lower DM and a higher linearity in comparison with the AU pectin.
32771505	0	28	dep	pectin	86:91	arg1	the					82:84	the	82:84	the	82:84	Characterization and swelling properties of composite gel microparticles based on the pectin and κ-carrageenan.
32771505	5	29	theme	gel	828:830	arg1	strength					832:839	increasing gel strength	817:839	increasing gel strength of the composite microparticles	817:871	Increasing concentration of the pectin promoted increasing gel strength of the composite microparticles.
32771505	9	30	theme	correlation	1324:1334	arg1	analysis					1336:1343	The correlation analysis	1320:1343	The correlation analysis	1320:1343	The correlation analysis indicated that increasing initial Ca2+ content and gel strength of the microparticles promoted decreasing swelling degree of the composite microparticles.
32771505	7	31	theme	higher	1086:1091	arg1	concentration					1100:1112	a higher pectin concentration	1084:1112	a higher pectin concentration	1084:1112	The microparticle gel formulations with a higher pectin concentration had a lower swelling degree in the simulated digestive fluids.
32771505	7	32	theme	pectin	1093:1098	arg1	concentration					1100:1112	a higher pectin concentration	1084:1112	a higher pectin concentration	1084:1112	The microparticle gel formulations with a higher pectin concentration had a lower swelling degree in the simulated digestive fluids.
32771505	6	33	theme	gel	885:887	arg1	DM					984:985	the lower DM	974:985	the lower DM	974:985	The higher gel strength of the composite microparticles based on the SVC pectin was probably due to the lower DM and a higher linearity in comparison with the AU pectin.
32771505	6	33	theme	gel	885:887	arg1	strength					889:896	The higher gel strength	874:896	The higher gel strength of the composite microparticles based on the SVC pectin	874:952	The higher gel strength of the composite microparticles based on the SVC pectin was probably due to the lower DM and a higher linearity in comparison with the AU pectin.
32771505	5	34	theme	microparticles	858:871	arg1	strength					832:839	increasing gel strength	817:839	increasing gel strength of the composite microparticles	817:871	Increasing concentration of the pectin promoted increasing gel strength of the composite microparticles.
32771505	1	35	theme	AU	253:254	arg1	pectins					257:263	commercial apple (AU) pectins	235:263	commercial apple (AU) pectins	235:263	The aim of this research was to produce composite gel microparticles based on the pectin (campion callus culture (SVC) and commercial apple (AU) pectins) and κ-carrageenan and investigate the relationship between the characteristics and swelling properties of the composite microparticles.
32771505	8	36	theme	carrageenan	1197:1207	arg1	addition					1181:1188	The addition	1177:1188	The addition of the carrageenan to the gel formulations	1177:1231	The addition of the carrageenan to the gel formulations led to an increase in the swelling degree in comparison with that without carrageenan.
32771505	3	37	theme	Ca2+	577:580	arg1	content					582:588	the Ca2+ content	573:588	the Ca2+ content	573:588	A significant positive correlation between the Ca2+ content and the SVC concentration in gel formulations was shown.
32771505	3	38	from	concentration	602:614	arg1	formulations					623:634	gel formulations	619:634	gel formulations	619:634	A significant positive correlation between the Ca2+ content and the SVC concentration in gel formulations was shown.
32771505	9	39	theme	microparticles	1484:1497	arg1	degree					1460:1465	decreasing swelling degree	1440:1465	decreasing swelling degree of the composite microparticles	1440:1497	The correlation analysis indicated that increasing initial Ca2+ content and gel strength of the microparticles promoted decreasing swelling degree of the composite microparticles.
32771505	0	40	theme	swelling	21:28	arg1	properties					30:39	swelling properties	21:39	swelling properties	21:39	Characterization and swelling properties of composite gel microparticles based on the pectin and κ-carrageenan.
32771505	7	41	theme	microparticle	1048:1060	arg1	formulations					1066:1077	The microparticle gel formulations	1044:1077	The microparticle gel formulations with a higher pectin concentration	1044:1112	The microparticle gel formulations with a higher pectin concentration had a lower swelling degree in the simulated digestive fluids.
32771505	3	42	dep	significant	532:542	arg1	positive					544:551	positive	544:551	positive	544:551	A significant positive correlation between the Ca2+ content and the SVC concentration in gel formulations was shown.
32771505	4	43	theme	Decreasing	647:656	arg1	degree					658:663	Decreasing degree	647:663	Decreasing degree of methyl esterification (DM) of the SVC pectin	647:711	Decreasing degree of methyl esterification (DM) of the SVC pectin promoted Ca2+ binding in comparison with the AU pectin.
32771505	0	44	theme	composite	44:52	arg1	microparticles					58:71	composite gel microparticles	44:71	composite gel microparticles	44:71	Characterization and swelling properties of composite gel microparticles based on the pectin and κ-carrageenan.
32771505	6	45	from	DM	984:985	arg1	comparison					1013:1022	comparison	1013:1022	comparison with the AU pectin	1013:1041	The higher gel strength of the composite microparticles based on the SVC pectin was probably due to the lower DM and a higher linearity in comparison with the AU pectin.
32771505	2	46	from	incubation	489:498	arg1	solution					520:527	calcium chloride solution	503:527	calcium chloride solution	503:527	The microparticles were obtained using emulsion dehydration techniques with successive incubation in calcium chloride solution.
32771505	6	47	with	comparison	1013:1022	arg1	pectin					1036:1041	the AU pectin	1029:1041	the AU pectin	1029:1041	The higher gel strength of the composite microparticles based on the SVC pectin was probably due to the lower DM and a higher linearity in comparison with the AU pectin.
32771505	4	48	theme	methyl	668:673	arg1	DM					691:692	DM	691:692	DM	691:692	Decreasing degree of methyl esterification (DM) of the SVC pectin promoted Ca2+ binding in comparison with the AU pectin.
32771505	4	48	theme	methyl	668:673	arg1	esterification					675:688	methyl esterification	668:688	methyl esterification (DM) of the SVC pectin	668:711	Decreasing degree of methyl esterification (DM) of the SVC pectin promoted Ca2+ binding in comparison with the AU pectin.
32771505	7	49	theme	simulated	1149:1157	arg1	fluids					1169:1174	the simulated digestive fluids	1145:1174	the simulated digestive fluids	1145:1174	The microparticle gel formulations with a higher pectin concentration had a lower swelling degree in the simulated digestive fluids.
32771505	9	50	theme	increasing	1360:1369	arg1	content					1384:1390	initial Ca2+ content	1371:1390	initial Ca2+ content	1371:1390	The correlation analysis indicated that increasing initial Ca2+ content and gel strength of the microparticles promoted decreasing swelling degree of the composite microparticles.
32771505	7	51	theme	gel	1062:1064	arg1	formulations					1066:1077	The microparticle gel formulations	1044:1077	The microparticle gel formulations with a higher pectin concentration	1044:1112	The microparticle gel formulations with a higher pectin concentration had a lower swelling degree in the simulated digestive fluids.
32771505	6	52	theme	higher	878:883	arg1	DM					984:985	the lower DM	974:985	the lower DM	974:985	The higher gel strength of the composite microparticles based on the SVC pectin was probably due to the lower DM and a higher linearity in comparison with the AU pectin.
32771505	6	52	theme	higher	878:883	arg1	strength					889:896	The higher gel strength	874:896	The higher gel strength of the composite microparticles based on the SVC pectin	874:952	The higher gel strength of the composite microparticles based on the SVC pectin was probably due to the lower DM and a higher linearity in comparison with the AU pectin.
32771505	3	53	theme	gel	619:621	arg1	formulations					623:634	gel formulations	619:634	gel formulations	619:634	A significant positive correlation between the Ca2+ content and the SVC concentration in gel formulations was shown.
32771505	8	54	from	increase	1243:1250	arg1	degree					1268:1273	the swelling degree	1255:1273	the swelling degree in comparison with that without carrageenan	1255:1317	The addition of the carrageenan to the gel formulations led to an increase in the swelling degree in comparison with that without carrageenan.
32771505	1	55	theme	campion	202:208	arg1	SVC					226:228	campion callus culture (SVC)	202:229	campion callus culture (SVC)	202:229	The aim of this research was to produce composite gel microparticles based on the pectin (campion callus culture (SVC) and commercial apple (AU) pectins) and κ-carrageenan and investigate the relationship between the characteristics and swelling properties of the composite microparticles.
32771505	0	56	theme	microparticles	58:71	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and swelling properties of composite gel microparticles based on the pectin and κ-carrageenan.
32771505	0	56	theme	microparticles	58:71	arg1	properties					30:39	swelling properties	21:39	swelling properties	21:39	Characterization and swelling properties of composite gel microparticles based on the pectin and κ-carrageenan.
32771505	9	57	theme	initial	1371:1377	arg1	content					1384:1390	initial Ca2+ content	1371:1390	initial Ca2+ content	1371:1390	The correlation analysis indicated that increasing initial Ca2+ content and gel strength of the microparticles promoted decreasing swelling degree of the composite microparticles.
32771505	1	58	theme	composite	376:384	arg1	microparticles					386:399	the composite microparticles	372:399	the composite microparticles	372:399	The aim of this research was to produce composite gel microparticles based on the pectin (campion callus culture (SVC) and commercial apple (AU) pectins) and κ-carrageenan and investigate the relationship between the characteristics and swelling properties of the composite microparticles.
32771505	3	59	theme	SVC	598:600	arg1	concentration					602:614	the SVC concentration	594:614	the SVC concentration	594:614	A significant positive correlation between the Ca2+ content and the SVC concentration in gel formulations was shown.
32771505	9	60	theme	swelling	1451:1458	arg1	degree					1460:1465	decreasing swelling degree	1440:1465	decreasing swelling degree of the composite microparticles	1440:1497	The correlation analysis indicated that increasing initial Ca2+ content and gel strength of the microparticles promoted decreasing swelling degree of the composite microparticles.
32771505	4	61	theme	Ca2+	722:725	arg1	binding					727:733	Ca2+ binding	722:733	Ca2+ binding	722:733	Decreasing degree of methyl esterification (DM) of the SVC pectin promoted Ca2+ binding in comparison with the AU pectin.
32771505	0	62	theme	gel	54:56	arg1	microparticles					58:71	composite gel microparticles	44:71	composite gel microparticles	44:71	Characterization and swelling properties of composite gel microparticles based on the pectin and κ-carrageenan.
32771505	9	63	theme	Ca2+	1379:1382	arg1	content					1384:1390	initial Ca2+ content	1371:1390	initial Ca2+ content	1371:1390	The correlation analysis indicated that increasing initial Ca2+ content and gel strength of the microparticles promoted decreasing swelling degree of the composite microparticles.
32771505	7	64	from	degree	1135:1140	arg1	fluids					1169:1174	the simulated digestive fluids	1145:1174	the simulated digestive fluids	1145:1174	The microparticle gel formulations with a higher pectin concentration had a lower swelling degree in the simulated digestive fluids.
32771505	1	65	theme	microparticles	386:399	arg1	characteristics					329:343	characteristics	329:343	characteristics	329:343	The aim of this research was to produce composite gel microparticles based on the pectin (campion callus culture (SVC) and commercial apple (AU) pectins) and κ-carrageenan and investigate the relationship between the characteristics and swelling properties of the composite microparticles.
32771505	1	65	theme	microparticles	386:399	arg1	properties					358:367	swelling properties	349:367	swelling properties	349:367	The aim of this research was to produce composite gel microparticles based on the pectin (campion callus culture (SVC) and commercial apple (AU) pectins) and κ-carrageenan and investigate the relationship between the characteristics and swelling properties of the composite microparticles.
32771505	8	66	with	comparison	1278:1287	arg1	that					1294:1297	that	1294:1297	that	1294:1297	The addition of the carrageenan to the gel formulations led to an increase in the swelling degree in comparison with that without carrageenan.
32771505	1	67	dep	characteristics	329:343	arg1	the					325:327	the	325:327	the	325:327	The aim of this research was to produce composite gel microparticles based on the pectin (campion callus culture (SVC) and commercial apple (AU) pectins) and κ-carrageenan and investigate the relationship between the characteristics and swelling properties of the composite microparticles.
32771505	3	68	from	content	582:588	arg1	formulations					623:634	gel formulations	619:634	gel formulations	619:634	A significant positive correlation between the Ca2+ content and the SVC concentration in gel formulations was shown.
32771505	1	69	theme	callus	210:215	arg1	SVC					226:228	campion callus culture (SVC)	202:229	campion callus culture (SVC)	202:229	The aim of this research was to produce composite gel microparticles based on the pectin (campion callus culture (SVC) and commercial apple (AU) pectins) and κ-carrageenan and investigate the relationship between the characteristics and swelling properties of the composite microparticles.
32771505	9	70	theme	microparticles	1416:1429	arg1	strength					1400:1407	gel strength	1396:1407	gel strength	1396:1407	The correlation analysis indicated that increasing initial Ca2+ content and gel strength of the microparticles promoted decreasing swelling degree of the composite microparticles.
32771505	9	70	theme	microparticles	1416:1429	arg1	content					1384:1390	initial Ca2+ content	1371:1390	initial Ca2+ content	1371:1390	The correlation analysis indicated that increasing initial Ca2+ content and gel strength of the microparticles promoted decreasing swelling degree of the composite microparticles.
32771505	8	71	from	degree	1268:1273	arg1	comparison					1278:1287	comparison	1278:1287	comparison with that without carrageenan	1278:1317	The addition of the carrageenan to the gel formulations led to an increase in the swelling degree in comparison with that without carrageenan.
32771505	1	72	theme	research	128:135	arg1	aim					116:118	The aim	112:118	The aim of this research	112:135	The aim of this research was to produce composite gel microparticles based on the pectin (campion callus culture (SVC) and commercial apple (AU) pectins) and κ-carrageenan and investigate the relationship between the characteristics and swelling properties of the composite microparticles.
32771505	1	73	theme	culture	217:223	arg1	SVC					226:228	campion callus culture (SVC)	202:229	campion callus culture (SVC)	202:229	The aim of this research was to produce composite gel microparticles based on the pectin (campion callus culture (SVC) and commercial apple (AU) pectins) and κ-carrageenan and investigate the relationship between the characteristics and swelling properties of the composite microparticles.
32771505	2	74	theme	chloride	511:518	arg1	solution					520:527	calcium chloride solution	503:527	calcium chloride solution	503:527	The microparticles were obtained using emulsion dehydration techniques with successive incubation in calcium chloride solution.
32771505	6	75	theme	AU	1033:1034	arg1	pectin					1036:1041	the AU pectin	1029:1041	the AU pectin	1029:1041	The higher gel strength of the composite microparticles based on the SVC pectin was probably due to the lower DM and a higher linearity in comparison with the AU pectin.
32771505	8	76	theme	swelling	1259:1266	arg1	degree					1268:1273	the swelling degree	1255:1273	the swelling degree in comparison with that without carrageenan	1255:1317	The addition of the carrageenan to the gel formulations led to an increase in the swelling degree in comparison with that without carrageenan.
32771505	9	77	theme	composite	1474:1482	arg1	microparticles					1484:1497	the composite microparticles	1470:1497	the composite microparticles	1470:1497	The correlation analysis indicated that increasing initial Ca2+ content and gel strength of the microparticles promoted decreasing swelling degree of the composite microparticles.
32771505	4	78	with	comparison	738:747	arg1	pectin					761:766	the AU pectin	754:766	the AU pectin	754:766	Decreasing degree of methyl esterification (DM) of the SVC pectin promoted Ca2+ binding in comparison with the AU pectin.
32771505	2	79	theme	calcium	503:509	arg1	solution					520:527	calcium chloride solution	503:527	calcium chloride solution	503:527	The microparticles were obtained using emulsion dehydration techniques with successive incubation in calcium chloride solution.
32771505	6	80	theme	SVC	943:945	arg1	pectin					947:952	the SVC pectin	939:952	the SVC pectin	939:952	The higher gel strength of the composite microparticles based on the SVC pectin was probably due to the lower DM and a higher linearity in comparison with the AU pectin.
32771505	1	81	dep	pectin	194:199	arg1	pectins					257:263	commercial apple (AU) pectins	235:263	commercial apple (AU) pectins	235:263	The aim of this research was to produce composite gel microparticles based on the pectin (campion callus culture (SVC) and commercial apple (AU) pectins) and κ-carrageenan and investigate the relationship between the characteristics and swelling properties of the composite microparticles.
32771505	1	81	dep	pectin	194:199	arg1	SVC					226:228	campion callus culture (SVC)	202:229	campion callus culture (SVC)	202:229	The aim of this research was to produce composite gel microparticles based on the pectin (campion callus culture (SVC) and commercial apple (AU) pectins) and κ-carrageenan and investigate the relationship between the characteristics and swelling properties of the composite microparticles.
32771505	3	82	theme	significant	532:542	arg1	correlation					553:563	A significant positive correlation	530:563	A significant positive correlation between the Ca2+ content and the SVC concentration in gel formulations	530:634	A significant positive correlation between the Ca2+ content and the SVC concentration in gel formulations was shown.
31896575	6	0	from	spectroscopy	771:782	arg1	complex					864:870	each species complex	851:870	each species complex	851:870	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	10	1	theme	chitin	1571:1576	arg1	rigidity					1549:1556	the relative rigidity	1536:1556	the relative rigidity of cell-wall chitin	1536:1576	3) Changes in the relative rigidity of cell-wall chitin were associated with aberrant pigment retention, implicating cell-wall flexibility as an independent variable in cryptococcal melanin assembly.
31896575	2	2	theme	melanin	377:383	arg1	production					339:348	production	339:348	production of the protective compound melanin	339:383	These two complexes share several phenotypic traits, such as production of the protective compound melanin.
31896575	9	3	theme	nonpolysaccharide	1449:1465	arg1	constituents					1467:1478	other nonpolysaccharide constituents	1443:1478	other nonpolysaccharide constituents that depended on the species or mutation	1443:1519	2) Strains from both species manifesting the leaky melanin phenotype had reduced chitosan content, which was compensated for by the production of lipids and other nonpolysaccharide constituents that depended on the species or mutation.
31896575	6	4	theme	strains	838:844	arg1	EM					727:728	transmission EM	714:728	transmission EM	714:728	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	6	4	theme	strains	838:844	arg1	assays					743:748	biochemical assays	731:748	biochemical assays	731:748	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	6	4	theme	strains	838:844	arg1	spectroscopy					771:782	solid-state NMR spectroscopy	755:782	solid-state NMR spectroscopy	755:782	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	2	5	theme	phenotypic	312:321	arg1	traits					323:328	several phenotypic traits	304:328	several phenotypic traits	304:328	These two complexes share several phenotypic traits, such as production of the protective compound melanin.
31896575	2	5	theme	phenotypic	312:321	arg1	production					339:348	production	339:348	production of the protective compound melanin	339:383	These two complexes share several phenotypic traits, such as production of the protective compound melanin.
31896575	6	6	theme	cell-wall	934:942	arg1	deposition					952:961	cell-wall pigment deposition	934:961	cell-wall pigment deposition	934:961	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	8	7	dep	had	1056:1058	arg1	1					1038:1038	1	1038:1038	1	1038:1038	1) C. gattii R265 had an exceptionally high chitosan content compared with C. neoformans H99; a rich chitosan composition promoted homogeneous melanin distribution throughout the cell wall but did not increase the propensity of pigment deposition.
31896575	6	8	theme	"	829:829	arg1	strains					838:844	"leaky melanin" mutant strains	815:844	"leaky melanin" mutant strains	815:844	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	1	9	theme	fungal	184:189	arg1	Cryptococcus					197:208	the large fungal genus Cryptococcus	174:208	the large fungal genus Cryptococcus	174:208	Cryptococcus neoformans and Cryptococcus gattii are two species complexes in the large fungal genus Cryptococcus and are responsible for potentially lethal disseminated infections.
31896575	10	10	from	variable	1679:1686	arg1	assembly					1712:1719	cryptococcal melanin assembly	1691:1719	cryptococcal melanin assembly	1691:1719	3) Changes in the relative rigidity of cell-wall chitin were associated with aberrant pigment retention, implicating cell-wall flexibility as an independent variable in cryptococcal melanin assembly.
31896575	6	11	theme	leaky	816:820	arg1	strains					838:844	"leaky melanin" mutant strains	815:844	"leaky melanin" mutant strains	815:844	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	11	12	from	anchoring	1835:1843	arg1	complexes					1925:1933	both C. neoformans and C. gattii species complexes	1884:1933	both C. neoformans and C. gattii species complexes	1884:1933	Overall, our results indicate that cell-wall composition and molecular architecture are critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes.
31896575	8	13	theme	gattii	1044:1049	arg1	R265					1051:1054	C. gattii R265	1041:1054	C. gattii R265	1041:1054	1) C. gattii R265 had an exceptionally high chitosan content compared with C. neoformans H99; a rich chitosan composition promoted homogeneous melanin distribution throughout the cell wall but did not increase the propensity of pigment deposition.
31896575	6	14	from	EM	727:728	arg1	complex					864:870	each species complex	851:870	each species complex	851:870	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	11	15	theme	melanin	1864:1870	arg1	pigments					1872:1879	melanin pigments	1864:1879	melanin pigments	1864:1879	Overall, our results indicate that cell-wall composition and molecular architecture are critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes.
31896575	11	16	from	arrangement	1849:1859	arg1	complexes					1925:1933	both C. neoformans and C. gattii species complexes	1884:1933	both C. neoformans and C. gattii species complexes	1884:1933	Overall, our results indicate that cell-wall composition and molecular architecture are critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes.
31896575	8	17	theme	pigment	1266:1272	arg1	deposition					1274:1283	pigment deposition	1266:1283	pigment deposition	1266:1283	1) C. gattii R265 had an exceptionally high chitosan content compared with C. neoformans H99; a rich chitosan composition promoted homogeneous melanin distribution throughout the cell wall but did not increase the propensity of pigment deposition.
31896575	8	18	dep	C.	1113:1114	arg1	neoformans					1116:1125	neoformans	1116:1125	neoformans	1116:1125	1) C. gattii R265 had an exceptionally high chitosan content compared with C. neoformans H99; a rich chitosan composition promoted homogeneous melanin distribution throughout the cell wall but did not increase the propensity of pigment deposition.
31896575	11	19	dep	anchoring	1835:1843	arg1	the					1831:1833	the	1831:1833	the	1831:1833	Overall, our results indicate that cell-wall composition and molecular architecture are critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes.
31896575	11	20	theme	critical	1810:1817	arg1	composition					1767:1777	cell-wall composition	1757:1777	cell-wall composition	1757:1777	Overall, our results indicate that cell-wall composition and molecular architecture are critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes.
31896575	11	20	theme	critical	1810:1817	arg1	architecture					1793:1804	molecular architecture	1783:1804	molecular architecture	1783:1804	Overall, our results indicate that cell-wall composition and molecular architecture are critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes.
31896575	11	20	theme	critical	1810:1817	arg1	factors					1819:1825	critical factors	1810:1825	critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes	1810:1933	Overall, our results indicate that cell-wall composition and molecular architecture are critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes.
31896575	0	21	theme	cell-wall	58:66	arg1	composition					68:78	cell-wall composition	58:78	cell-wall composition	58:78	Melanin deposition in two Cryptococcus species depends on cell-wall composition and flexibility.
31896575	9	22	theme	leaky	1331:1335	arg1	phenotype					1345:1353	the leaky melanin phenotype	1327:1353	the leaky melanin phenotype	1327:1353	2) Strains from both species manifesting the leaky melanin phenotype had reduced chitosan content, which was compensated for by the production of lipids and other nonpolysaccharide constituents that depended on the species or mutation.
31896575	5	23	theme	similar	634:640	arg1	factors					642:648	similar factors	634:648	similar factors	634:648	However, whether similar factors influence melanization in C. gattii is unknown.
31896575	6	24	used	used	709:712	arg2	we					706:707	we	706:707	we	706:707	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	9	25	theme	reduced	1359:1365	arg1	content					1376:1382	reduced chitosan content	1359:1382	reduced chitosan content	1359:1382	2) Strains from both species manifesting the leaky melanin phenotype had reduced chitosan content, which was compensated for by the production of lipids and other nonpolysaccharide constituents that depended on the species or mutation.
31896575	8	26	theme	chitosan	1082:1089	arg1	content					1091:1097	an exceptionally high chitosan content	1060:1097	an exceptionally high chitosan content	1060:1097	1) C. gattii R265 had an exceptionally high chitosan content compared with C. neoformans H99; a rich chitosan composition promoted homogeneous melanin distribution throughout the cell wall but did not increase the propensity of pigment deposition.
31896575	10	27	theme	melanin	1704:1710	arg1	assembly					1712:1719	cryptococcal melanin assembly	1691:1719	cryptococcal melanin assembly	1691:1719	3) Changes in the relative rigidity of cell-wall chitin were associated with aberrant pigment retention, implicating cell-wall flexibility as an independent variable in cryptococcal melanin assembly.
31896575	0	28	theme	Melanin	0:6	arg1	deposition					8:17	Melanin deposition	0:17	Melanin deposition in two Cryptococcus species	0:45	Melanin deposition in two Cryptococcus species depends on cell-wall composition and flexibility.
31896575	1	29	theme	Cryptococcus	97:108	arg1	complexes					161:169	two species complexes	149:169	two species complexes in the large fungal genus Cryptococcus	149:208	Cryptococcus neoformans and Cryptococcus gattii are two species complexes in the large fungal genus Cryptococcus and are responsible for potentially lethal disseminated infections.
31896575	1	29	theme	Cryptococcus	97:108	arg1	neoformans					110:119	Cryptococcus neoformans	97:119	Cryptococcus neoformans	97:119	Cryptococcus neoformans and Cryptococcus gattii are two species complexes in the large fungal genus Cryptococcus and are responsible for potentially lethal disseminated infections.
31896575	1	29	theme	Cryptococcus	97:108	arg1	gattii					138:143	Cryptococcus gattii	125:143	Cryptococcus gattii	125:143	Cryptococcus neoformans and Cryptococcus gattii are two species complexes in the large fungal genus Cryptococcus and are responsible for potentially lethal disseminated infections.
31896575	4	30	theme	cell-wall	576:584	arg1	composition					586:596	cell-wall composition	576:596	cell-wall composition	576:596	Consequently, melanization is modulated by changes in cell-wall composition or ultrastructure.
31896575	6	31	theme	transmission	714:725	arg1	EM					727:728	transmission EM	714:728	transmission EM	714:728	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	8	32	theme	C.	1113:1114	arg1	H99					1127:1129	C. neoformans H99	1113:1129	C. neoformans H99	1113:1129	1) C. gattii R265 had an exceptionally high chitosan content compared with C. neoformans H99; a rich chitosan composition promoted homogeneous melanin distribution throughout the cell wall but did not increase the propensity of pigment deposition.
31896575	6	33	theme	structural	905:914	arg1	factors					916:922	the compositional and structural factors	883:922	the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii	883:992	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	3	34	theme	key	432:434	arg1	constituents					445:456	key cellular constituents	432:456	key cellular constituents that are essential for melanin deposition within the cell wall	432:519	In C. neoformans, the pigment associates with key cellular constituents that are essential for melanin deposition within the cell wall.
31896575	8	35	contain	had	1056:1058	arg2	content					1091:1097	an exceptionally high chitosan content	1060:1097	an exceptionally high chitosan content	1060:1097	1) C. gattii R265 had an exceptionally high chitosan content compared with C. neoformans H99; a rich chitosan composition promoted homogeneous melanin distribution throughout the cell wall but did not increase the propensity of pigment deposition.
31896575	8	35	contain	had	1056:1058	arg1	R265					1051:1054	C. gattii R265	1041:1054	C. gattii R265	1041:1054	1) C. gattii R265 had an exceptionally high chitosan content compared with C. neoformans H99; a rich chitosan composition promoted homogeneous melanin distribution throughout the cell wall but did not increase the propensity of pigment deposition.
31896575	4	36	from	changes	565:571	arg1	composition					586:596	cell-wall composition	576:596	cell-wall composition	576:596	Consequently, melanization is modulated by changes in cell-wall composition or ultrastructure.
31896575	4	36	from	changes	565:571	arg1	ultrastructure					601:614	ultrastructure	601:614	ultrastructure	601:614	Consequently, melanization is modulated by changes in cell-wall composition or ultrastructure.
31896575	0	37	theme	Cryptococcus	26:37	arg1	species					39:45	two Cryptococcus species	22:45	two Cryptococcus species	22:45	Melanin deposition in two Cryptococcus species depends on cell-wall composition and flexibility.
31896575	6	38	theme	compositional	887:899	arg1	factors					916:922	the compositional and structural factors	883:922	the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii	883:992	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	10	39	theme	aberrant	1599:1606	arg1	retention					1616:1624	aberrant pigment retention	1599:1624	aberrant pigment retention	1599:1624	3) Changes in the relative rigidity of cell-wall chitin were associated with aberrant pigment retention, implicating cell-wall flexibility as an independent variable in cryptococcal melanin assembly.
31896575	10	40	theme	cell-wall	1561:1569	arg1	chitin					1571:1576	cell-wall chitin	1561:1576	cell-wall chitin	1561:1576	3) Changes in the relative rigidity of cell-wall chitin were associated with aberrant pigment retention, implicating cell-wall flexibility as an independent variable in cryptococcal melanin assembly.
31896575	6	41	theme	isolates	802:809	arg1	EM					727:728	transmission EM	714:728	transmission EM	714:728	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	6	41	theme	isolates	802:809	arg1	assays					743:748	biochemical assays	731:748	biochemical assays	731:748	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	6	41	theme	isolates	802:809	arg1	spectroscopy					771:782	solid-state NMR spectroscopy	755:782	solid-state NMR spectroscopy	755:782	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	2	42	theme	compound	368:375	arg1	melanin					377:383	the protective compound melanin	353:383	the protective compound melanin	353:383	These two complexes share several phenotypic traits, such as production of the protective compound melanin.
31896575	9	43	dep	had	1355:1357	arg1	2					1286:1286	2	1286:1286	2	1286:1286	2) Strains from both species manifesting the leaky melanin phenotype had reduced chitosan content, which was compensated for by the production of lipids and other nonpolysaccharide constituents that depended on the species or mutation.
31896575	11	44	theme	gattii	1910:1915	arg1	complexes					1925:1933	both C. neoformans and C. gattii species complexes	1884:1933	both C. neoformans and C. gattii species complexes	1884:1933	Overall, our results indicate that cell-wall composition and molecular architecture are critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes.
31896575	8	45	theme	chitosan	1139:1146	arg1	composition					1148:1158	a rich chitosan composition	1132:1158	a rich chitosan composition	1132:1158	1) C. gattii R265 had an exceptionally high chitosan content compared with C. neoformans H99; a rich chitosan composition promoted homogeneous melanin distribution throughout the cell wall but did not increase the propensity of pigment deposition.
31896575	11	46	theme	molecular	1783:1791	arg1	composition					1767:1777	cell-wall composition	1757:1777	cell-wall composition	1757:1777	Overall, our results indicate that cell-wall composition and molecular architecture are critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes.
31896575	11	46	theme	molecular	1783:1791	arg1	architecture					1793:1804	molecular architecture	1783:1804	molecular architecture	1783:1804	Overall, our results indicate that cell-wall composition and molecular architecture are critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes.
31896575	11	46	theme	molecular	1783:1791	arg1	factors					1819:1825	critical factors	1810:1825	critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes	1810:1933	Overall, our results indicate that cell-wall composition and molecular architecture are critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes.
31896575	6	47	theme	NMR	767:769	arg1	spectroscopy					771:782	solid-state NMR spectroscopy	755:782	solid-state NMR spectroscopy	755:782	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	9	48	theme	lipids	1432:1437	arg1	production					1418:1427	the production	1414:1427	the production of lipids and other nonpolysaccharide constituents that depended on the species or mutation	1414:1519	2) Strains from both species manifesting the leaky melanin phenotype had reduced chitosan content, which was compensated for by the production of lipids and other nonpolysaccharide constituents that depended on the species or mutation.
31896575	9	49	from	species	1307:1313	arg1	Strains					1289:1295	Strains	1289:1295	Strains from both species manifesting the leaky melanin phenotype	1289:1353	2) Strains from both species manifesting the leaky melanin phenotype had reduced chitosan content, which was compensated for by the production of lipids and other nonpolysaccharide constituents that depended on the species or mutation.
31896575	9	50	theme	other	1443:1447	arg1	constituents					1467:1478	other nonpolysaccharide constituents	1443:1478	other nonpolysaccharide constituents that depended on the species or mutation	1443:1519	2) Strains from both species manifesting the leaky melanin phenotype had reduced chitosan content, which was compensated for by the production of lipids and other nonpolysaccharide constituents that depended on the species or mutation.
31896575	8	51	theme	melanin	1181:1187	arg1	distribution					1189:1200	homogeneous melanin distribution	1169:1200	homogeneous melanin distribution	1169:1200	1) C. gattii R265 had an exceptionally high chitosan content compared with C. neoformans H99; a rich chitosan composition promoted homogeneous melanin distribution throughout the cell wall but did not increase the propensity of pigment deposition.
31896575	9	52	theme	constituents	1467:1478	arg1	production					1418:1427	the production	1414:1427	the production of lipids and other nonpolysaccharide constituents that depended on the species or mutation	1414:1519	2) Strains from both species manifesting the leaky melanin phenotype had reduced chitosan content, which was compensated for by the production of lipids and other nonpolysaccharide constituents that depended on the species or mutation.
31896575	6	53	theme	pigment	944:950	arg1	deposition					952:961	cell-wall pigment deposition	934:961	cell-wall pigment deposition	934:961	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	10	54	theme	pigment	1608:1614	arg1	retention					1616:1624	aberrant pigment retention	1599:1624	aberrant pigment retention	1599:1624	3) Changes in the relative rigidity of cell-wall chitin were associated with aberrant pigment retention, implicating cell-wall flexibility as an independent variable in cryptococcal melanin assembly.
31896575	6	55	theme	mutant	831:836	arg1	strains					838:844	"leaky melanin" mutant strains	815:844	"leaky melanin" mutant strains	815:844	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	2	56	theme	several	304:310	arg1	traits					323:328	several phenotypic traits	304:328	several phenotypic traits	304:328	These two complexes share several phenotypic traits, such as production of the protective compound melanin.
31896575	2	56	theme	several	304:310	arg1	production					339:348	production	339:348	production of the protective compound melanin	339:383	These two complexes share several phenotypic traits, such as production of the protective compound melanin.
31896575	1	57	theme	large	178:182	arg1	Cryptococcus					197:208	the large fungal genus Cryptococcus	174:208	the large fungal genus Cryptococcus	174:208	Cryptococcus neoformans and Cryptococcus gattii are two species complexes in the large fungal genus Cryptococcus and are responsible for potentially lethal disseminated infections.
31896575	8	58	theme	cell	1217:1220	arg1	wall					1222:1225	the cell wall	1213:1225	the cell wall	1213:1225	1) C. gattii R265 had an exceptionally high chitosan content compared with C. neoformans H99; a rich chitosan composition promoted homogeneous melanin distribution throughout the cell wall but did not increase the propensity of pigment deposition.
31896575	8	59	theme	C.	1041:1042	arg1	R265					1051:1054	C. gattii R265	1041:1054	C. gattii R265	1041:1054	1) C. gattii R265 had an exceptionally high chitosan content compared with C. neoformans H99; a rich chitosan composition promoted homogeneous melanin distribution throughout the cell wall but did not increase the propensity of pigment deposition.
31896575	6	60	from	assays	743:748	arg1	complex					864:870	each species complex	851:870	each species complex	851:870	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	8	61	theme	deposition	1274:1283	arg1	propensity					1252:1261	the propensity	1248:1261	the propensity of pigment deposition	1248:1283	1) C. gattii R265 had an exceptionally high chitosan content compared with C. neoformans H99; a rich chitosan composition promoted homogeneous melanin distribution throughout the cell wall but did not increase the propensity of pigment deposition.
31896575	6	62	theme	melanin	822:828	arg1	strains					838:844	"leaky melanin" mutant strains	815:844	"leaky melanin" mutant strains	815:844	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	1	63	theme	genus	191:195	arg1	Cryptococcus					197:208	the large fungal genus Cryptococcus	174:208	the large fungal genus Cryptococcus	174:208	Cryptococcus neoformans and Cryptococcus gattii are two species complexes in the large fungal genus Cryptococcus and are responsible for potentially lethal disseminated infections.
31896575	10	64	theme	cell-wall	1639:1647	arg1	flexibility					1649:1659	cell-wall flexibility	1639:1659	cell-wall flexibility	1639:1659	3) Changes in the relative rigidity of cell-wall chitin were associated with aberrant pigment retention, implicating cell-wall flexibility as an independent variable in cryptococcal melanin assembly.
31896575	10	64	theme	cell-wall	1639:1647	arg1	variable					1679:1686	an independent variable	1664:1686	an independent variable in cryptococcal melanin assembly	1664:1719	3) Changes in the relative rigidity of cell-wall chitin were associated with aberrant pigment retention, implicating cell-wall flexibility as an independent variable in cryptococcal melanin assembly.
31896575	6	65	from	complex	864:870	arg1	isolates					802:809	representative isolates	787:809	representative isolates	787:809	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	6	65	from	complex	864:870	arg1	EM					727:728	transmission EM	714:728	transmission EM	714:728	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	6	65	from	complex	864:870	arg1	assays					743:748	biochemical assays	731:748	biochemical assays	731:748	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	6	65	from	complex	864:870	arg1	spectroscopy					771:782	solid-state NMR spectroscopy	755:782	solid-state NMR spectroscopy	755:782	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	6	65	from	complex	864:870	arg1	strains					838:844	"leaky melanin" mutant strains	815:844	"leaky melanin" mutant strains	815:844	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	11	66	theme	pigments	1872:1879	arg1	arrangement					1849:1859	arrangement	1849:1859	arrangement	1849:1859	Overall, our results indicate that cell-wall composition and molecular architecture are critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes.
31896575	11	66	theme	pigments	1872:1879	arg1	anchoring					1835:1843	anchoring	1835:1843	anchoring	1835:1843	Overall, our results indicate that cell-wall composition and molecular architecture are critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes.
31896575	10	67	theme	independent	1667:1677	arg1	flexibility					1649:1659	cell-wall flexibility	1639:1659	cell-wall flexibility	1639:1659	3) Changes in the relative rigidity of cell-wall chitin were associated with aberrant pigment retention, implicating cell-wall flexibility as an independent variable in cryptococcal melanin assembly.
31896575	10	67	theme	independent	1667:1677	arg1	variable					1679:1686	an independent variable	1664:1686	an independent variable in cryptococcal melanin assembly	1664:1719	3) Changes in the relative rigidity of cell-wall chitin were associated with aberrant pigment retention, implicating cell-wall flexibility as an independent variable in cryptococcal melanin assembly.
31896575	1	68	theme	Cryptococcus	125:136	arg1	complexes					161:169	two species complexes	149:169	two species complexes in the large fungal genus Cryptococcus	149:208	Cryptococcus neoformans and Cryptococcus gattii are two species complexes in the large fungal genus Cryptococcus and are responsible for potentially lethal disseminated infections.
31896575	1	68	theme	Cryptococcus	125:136	arg1	neoformans					110:119	Cryptococcus neoformans	97:119	Cryptococcus neoformans	97:119	Cryptococcus neoformans and Cryptococcus gattii are two species complexes in the large fungal genus Cryptococcus and are responsible for potentially lethal disseminated infections.
31896575	1	68	theme	Cryptococcus	125:136	arg1	gattii					138:143	Cryptococcus gattii	125:143	Cryptococcus gattii	125:143	Cryptococcus neoformans and Cryptococcus gattii are two species complexes in the large fungal genus Cryptococcus and are responsible for potentially lethal disseminated infections.
31896575	11	69	theme	neoformans	1892:1901	arg1	complexes					1925:1933	both C. neoformans and C. gattii species complexes	1884:1933	both C. neoformans and C. gattii species complexes	1884:1933	Overall, our results indicate that cell-wall composition and molecular architecture are critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes.
31896575	10	70	from	Changes	1525:1531	arg1	rigidity					1549:1556	the relative rigidity	1536:1556	the relative rigidity of cell-wall chitin	1536:1576	3) Changes in the relative rigidity of cell-wall chitin were associated with aberrant pigment retention, implicating cell-wall flexibility as an independent variable in cryptococcal melanin assembly.
31896575	1	71	theme	species	153:159	arg1	complexes					161:169	two species complexes	149:169	two species complexes in the large fungal genus Cryptococcus	149:208	Cryptococcus neoformans and Cryptococcus gattii are two species complexes in the large fungal genus Cryptococcus and are responsible for potentially lethal disseminated infections.
31896575	1	71	theme	species	153:159	arg1	neoformans					110:119	Cryptococcus neoformans	97:119	Cryptococcus neoformans	97:119	Cryptococcus neoformans and Cryptococcus gattii are two species complexes in the large fungal genus Cryptococcus and are responsible for potentially lethal disseminated infections.
31896575	1	71	theme	species	153:159	arg1	gattii					138:143	Cryptococcus gattii	125:143	Cryptococcus gattii	125:143	Cryptococcus neoformans and Cryptococcus gattii are two species complexes in the large fungal genus Cryptococcus and are responsible for potentially lethal disseminated infections.
31896575	0	72	from	deposition	8:17	arg1	species					39:45	two Cryptococcus species	22:45	two Cryptococcus species	22:45	Melanin deposition in two Cryptococcus species depends on cell-wall composition and flexibility.
31896575	10	73	dep	Changes	1525:1531	arg1	3					1522:1522	3	1522:1522	3	1522:1522	3) Changes in the relative rigidity of cell-wall chitin were associated with aberrant pigment retention, implicating cell-wall flexibility as an independent variable in cryptococcal melanin assembly.
31896575	9	74	theme	melanin	1337:1343	arg1	phenotype					1345:1353	the leaky melanin phenotype	1327:1353	the leaky melanin phenotype	1327:1353	2) Strains from both species manifesting the leaky melanin phenotype had reduced chitosan content, which was compensated for by the production of lipids and other nonpolysaccharide constituents that depended on the species or mutation.
31896575	8	75	theme	high	1077:1080	arg1	content					1091:1097	an exceptionally high chitosan content	1060:1097	an exceptionally high chitosan content	1060:1097	1) C. gattii R265 had an exceptionally high chitosan content compared with C. neoformans H99; a rich chitosan composition promoted homogeneous melanin distribution throughout the cell wall but did not increase the propensity of pigment deposition.
31896575	11	76	theme	species	1917:1923	arg1	complexes					1925:1933	both C. neoformans and C. gattii species complexes	1884:1933	both C. neoformans and C. gattii species complexes	1884:1933	Overall, our results indicate that cell-wall composition and molecular architecture are critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes.
31896575	1	77	from	complexes	161:169	arg1	Cryptococcus					197:208	the large fungal genus Cryptococcus	174:208	the large fungal genus Cryptococcus	174:208	Cryptococcus neoformans and Cryptococcus gattii are two species complexes in the large fungal genus Cryptococcus and are responsible for potentially lethal disseminated infections.
31896575	6	78	theme	biochemical	731:741	arg1	assays					743:748	biochemical assays	731:748	biochemical assays	731:748	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	9	79	theme	chitosan	1367:1374	arg1	content					1376:1382	reduced chitosan content	1359:1382	reduced chitosan content	1359:1382	2) Strains from both species manifesting the leaky melanin phenotype had reduced chitosan content, which was compensated for by the production of lipids and other nonpolysaccharide constituents that depended on the species or mutation.
31896575	10	80	theme	cryptococcal	1691:1702	arg1	assembly					1712:1719	cryptococcal melanin assembly	1691:1719	cryptococcal melanin assembly	1691:1719	3) Changes in the relative rigidity of cell-wall chitin were associated with aberrant pigment retention, implicating cell-wall flexibility as an independent variable in cryptococcal melanin assembly.
31896575	7	81	theme	principal	999:1007	arg1	findings					1009:1016	The principal findings	995:1016	The principal findings	995:1016	The principal findings were the following.
31896575	7	81	theme	principal	999:1007	arg1	following					1027:1035	the following	1023:1035	the following	1023:1035	The principal findings were the following.
31896575	11	82	theme	cell-wall	1757:1765	arg1	composition					1767:1777	cell-wall composition	1757:1777	cell-wall composition	1757:1777	Overall, our results indicate that cell-wall composition and molecular architecture are critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes.
31896575	11	82	theme	cell-wall	1757:1765	arg1	architecture					1793:1804	molecular architecture	1783:1804	molecular architecture	1783:1804	Overall, our results indicate that cell-wall composition and molecular architecture are critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes.
31896575	11	82	theme	cell-wall	1757:1765	arg1	factors					1819:1825	critical factors	1810:1825	critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes	1810:1933	Overall, our results indicate that cell-wall composition and molecular architecture are critical factors for the anchoring and arrangement of melanin pigments in both C. neoformans and C. gattii species complexes.
31896575	9	83	contain	had	1355:1357	arg2	content					1376:1382	reduced chitosan content	1359:1382	reduced chitosan content	1359:1382	2) Strains from both species manifesting the leaky melanin phenotype had reduced chitosan content, which was compensated for by the production of lipids and other nonpolysaccharide constituents that depended on the species or mutation.
31896575	9	83	contain	had	1355:1357	arg1	Strains					1289:1295	Strains	1289:1295	Strains from both species manifesting the leaky melanin phenotype	1289:1353	2) Strains from both species manifesting the leaky melanin phenotype had reduced chitosan content, which was compensated for by the production of lipids and other nonpolysaccharide constituents that depended on the species or mutation.
31896575	3	84	theme	cellular	436:443	arg1	constituents					445:456	key cellular constituents	432:456	key cellular constituents that are essential for melanin deposition within the cell wall	432:519	In C. neoformans, the pigment associates with key cellular constituents that are essential for melanin deposition within the cell wall.
31896575	1	85	theme	disseminated	253:264	arg1	infections					266:275	potentially lethal disseminated infections	234:275	potentially lethal disseminated infections	234:275	Cryptococcus neoformans and Cryptococcus gattii are two species complexes in the large fungal genus Cryptococcus and are responsible for potentially lethal disseminated infections.
31896575	3	86	theme	melanin	481:487	arg1	deposition					489:498	melanin deposition	481:498	melanin deposition within the cell wall	481:519	In C. neoformans, the pigment associates with key cellular constituents that are essential for melanin deposition within the cell wall.
31896575	8	87	theme	rich	1134:1137	arg1	composition					1148:1158	a rich chitosan composition	1132:1158	a rich chitosan composition	1132:1158	1) C. gattii R265 had an exceptionally high chitosan content compared with C. neoformans H99; a rich chitosan composition promoted homogeneous melanin distribution throughout the cell wall but did not increase the propensity of pigment deposition.
31896575	6	88	theme	representative	787:800	arg1	isolates					802:809	representative isolates	787:809	representative isolates	787:809	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	2	89	theme	protective	357:366	arg1	melanin					377:383	the protective compound melanin	353:383	the protective compound melanin	353:383	These two complexes share several phenotypic traits, such as production of the protective compound melanin.
31896575	10	90	theme	relative	1540:1547	arg1	rigidity					1549:1556	the relative rigidity	1536:1556	the relative rigidity of cell-wall chitin	1536:1576	3) Changes in the relative rigidity of cell-wall chitin were associated with aberrant pigment retention, implicating cell-wall flexibility as an independent variable in cryptococcal melanin assembly.
31896575	3	91	theme	cell	511:514	arg1	wall					516:519	the cell wall	507:519	the cell wall	507:519	In C. neoformans, the pigment associates with key cellular constituents that are essential for melanin deposition within the cell wall.
31896575	1	92	theme	lethal	246:251	arg1	infections					266:275	potentially lethal disseminated infections	234:275	potentially lethal disseminated infections	234:275	Cryptococcus neoformans and Cryptococcus gattii are two species complexes in the large fungal genus Cryptococcus and are responsible for potentially lethal disseminated infections.
31896575	8	93	theme	homogeneous	1169:1179	arg1	distribution					1189:1200	homogeneous melanin distribution	1169:1200	homogeneous melanin distribution	1169:1200	1) C. gattii R265 had an exceptionally high chitosan content compared with C. neoformans H99; a rich chitosan composition promoted homogeneous melanin distribution throughout the cell wall but did not increase the propensity of pigment deposition.
31896575	6	94	theme	species	856:862	arg1	complex					864:870	each species complex	851:870	each species complex	851:870	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
31896575	6	95	theme	solid-state	755:765	arg1	spectroscopy					771:782	solid-state NMR spectroscopy	755:782	solid-state NMR spectroscopy	755:782	Herein, we used transmission EM, biochemical assays, and solid-state NMR spectroscopy of representative isolates and "leaky melanin" mutant strains from each species complex to examine the compositional and structural factors governing cell-wall pigment deposition in C. neoformans and C. gattii.
33202908	0	0	theme	PLA-Arbocel®	53:64	arg1	Biocomposites					66:78	PLA-Arbocel® Biocomposites	53:78	PLA-Arbocel® Biocomposites	53:78	Effect of a Bio-Based Dispersing Aid (Einar® 101) on PLA-Arbocel® Biocomposites: Evaluation of the Interfacial Shear Stress on the Final Mechanical Properties.
33202908	5	1	dep	bio-based	667:675	arg1	aid					688:690	aid	688:690	aid	688:690	Consequently, in order to obtain a composite with enhanced impact resistance and elongation at break, the effect of the Einar 101 addition (a bio-based dispersing aid additive) was analyzed.
33202908	8	2	theme	fiber-matrix	1120:1131	arg1	adhesion					1133:1140	the fiber-matrix adhesion	1116:1140	the fiber-matrix adhesion	1116:1140	Analytical models were also applied in order to obtain an evaluation of the variation of the interfacial shear stress (IFSS) (strictly correlated to the fiber-matrix adhesion) caused by the Einar introduction.
33202908	1	3	theme	poly	218:221	arg1	acid					232:235	poly (lactic) acid	218:235	poly (lactic) acid	218:235	In this paper, the production and the characterization of poly (lactic) acid (PLA)-based composites containing different amounts (from 10 wt.
33202908	4	4	theme	previous	409:416	arg1	study					418:422	a previous study	407:422	a previous study	407:422	On the basis of a previous study, it was observed that the addition of the cellulose fibers led to an embrittlement of the composite.
33202908	7	5	theme	Einar	933:937	arg1	addition					939:946	the Einar addition	929:946	the Einar addition	929:946	Also, the consequences on the final mechanical properties (tensile and impact test) caused by the Einar addition were investigated.
33202908	0	6	from	Effect	0:5	arg1	Biocomposites					66:78	PLA-Arbocel® Biocomposites	53:78	PLA-Arbocel® Biocomposites	53:78	Effect of a Bio-Based Dispersing Aid (Einar® 101) on PLA-Arbocel® Biocomposites: Evaluation of the Interfacial Shear Stress on the Final Mechanical Properties.
33202908	1	7	theme	lactic	224:229	arg1	acid					232:235	poly (lactic) acid	218:235	poly (lactic) acid	218:235	In this paper, the production and the characterization of poly (lactic) acid (PLA)-based composites containing different amounts (from 10 wt.
33202908	8	8	theme	Analytical	967:976	arg1	models					978:983	Analytical models	967:983	Analytical models	967:983	Analytical models were also applied in order to obtain an evaluation of the variation of the interfacial shear stress (IFSS) (strictly correlated to the fiber-matrix adhesion) caused by the Einar introduction.
33202908	8	9	dep	stress	1078:1083	arg1	correlated					1102:1111	correlated	1102:1111	correlated to the fiber-matrix adhesion	1102:1140	Analytical models were also applied in order to obtain an evaluation of the variation of the interfacial shear stress (IFSS) (strictly correlated to the fiber-matrix adhesion) caused by the Einar introduction.
33202908	4	10	theme	cellulose	466:474	arg1	fibers					476:481	the cellulose fibers	462:481	the cellulose fibers	462:481	On the basis of a previous study, it was observed that the addition of the cellulose fibers led to an embrittlement of the composite.
33202908	6	11	theme	adhesion	732:739	arg1	role					720:723	The role	716:723	The role	716:723	The role of the adhesion between the fiber and the matrix, coupled with a better fiber dispersion, was thus evaluated.
33202908	5	12	theme	Einar	645:649	arg1	effect					631:636	the effect	627:636	the effect of the Einar 101 addition (a bio-based dispersing aid additive)	627:700	Consequently, in order to obtain a composite with enhanced impact resistance and elongation at break, the effect of the Einar 101 addition (a bio-based dispersing aid additive) was analyzed.
33202908	9	13	theme	aspect	1210:1215	arg1	ratio					1217:1221	the very low aspect ratio	1197:1221	the very low aspect ratio of the Arbocel fibers	1197:1243	Furthermore, due to the very low aspect ratio of the Arbocel fibers, a suitable Bader and Boyer model variation was adopted in order to have a better quantitative estimation of the IFSS value.
33202908	1	14	dep	-based	242:247	arg1	acid					232:235	poly (lactic) acid	218:235	poly (lactic) acid	218:235	In this paper, the production and the characterization of poly (lactic) acid (PLA)-based composites containing different amounts (from 10 wt.
33202908	2	15	dep	%	301:301	arg1	wt					309:310	25 wt	306:310	% to 25 wt.	301:311	% to 25 wt.
33202908	2	15	dep	%	301:301	arg1	to					303:304	to	303:304	to	303:304	% to 25 wt.
33202908	5	16	with	composite	560:568	arg1	elongation					606:615	elongation	606:615	elongation	606:615	Consequently, in order to obtain a composite with enhanced impact resistance and elongation at break, the effect of the Einar 101 addition (a bio-based dispersing aid additive) was analyzed.
33202908	5	16	with	composite	560:568	arg1	resistance					591:600	enhanced impact resistance	575:600	enhanced impact resistance	575:600	Consequently, in order to obtain a composite with enhanced impact resistance and elongation at break, the effect of the Einar 101 addition (a bio-based dispersing aid additive) was analyzed.
33202908	7	17	theme	mechanical	871:880	arg1	test					913:916	impact test	906:916	impact test	906:916	Also, the consequences on the final mechanical properties (tensile and impact test) caused by the Einar addition were investigated.
33202908	7	17	theme	mechanical	871:880	arg1	properties					882:891	the final mechanical properties	861:891	the final mechanical properties (tensile and impact test) caused by the Einar addition	861:946	Also, the consequences on the final mechanical properties (tensile and impact test) caused by the Einar addition were investigated.
33202908	7	17	theme	mechanical	871:880	arg1	tensile					894:900	tensile	894:900	tensile	894:900	Also, the consequences on the final mechanical properties (tensile and impact test) caused by the Einar addition were investigated.
33202908	8	18	theme	Einar	1157:1161	arg1	introduction					1163:1174	the Einar introduction	1153:1174	the Einar introduction	1153:1174	Analytical models were also applied in order to obtain an evaluation of the variation of the interfacial shear stress (IFSS) (strictly correlated to the fiber-matrix adhesion) caused by the Einar introduction.
33202908	3	19	theme	fibers	340:345	arg1	fibers					340:345	ultra-short cellulose fibers	318:345	ultra-short cellulose fibers (Arbocel 600 BE/PU)	318:365	%) of ultra-short cellulose fibers (Arbocel 600 BE/PU) have been investigated.
33202908	3	19	theme	fibers	340:345	arg1	%					312:312	%)	312:313	%) of ultra-short cellulose fibers (Arbocel 600 BE/PU)	312:365	%) of ultra-short cellulose fibers (Arbocel 600 BE/PU) have been investigated.
33202908	3	19	theme	fibers	340:345	arg1	BE/PU					360:364	BE/PU	360:364	BE/PU	360:364	%) of ultra-short cellulose fibers (Arbocel 600 BE/PU) have been investigated.
33202908	5	20	theme	impact	584:589	arg1	resistance					591:600	enhanced impact resistance	575:600	enhanced impact resistance	575:600	Consequently, in order to obtain a composite with enhanced impact resistance and elongation at break, the effect of the Einar 101 addition (a bio-based dispersing aid additive) was analyzed.
33202908	5	21	dep	Einar	645:649	arg1	addition					655:662	addition	655:662	the Einar 101 addition (a bio-based dispersing aid additive)	641:700	Consequently, in order to obtain a composite with enhanced impact resistance and elongation at break, the effect of the Einar 101 addition (a bio-based dispersing aid additive) was analyzed.
33202908	5	21	dep	Einar	645:649	arg1	bio-based					667:675	bio-based	667:675	bio-based	667:675	Consequently, in order to obtain a composite with enhanced impact resistance and elongation at break, the effect of the Einar 101 addition (a bio-based dispersing aid additive) was analyzed.
33202908	9	22	theme	low	1206:1208	arg1	aspect					1210:1215	the very low aspect	1197:1215	the very low aspect ratio of the Arbocel fibers	1197:1243	Furthermore, due to the very low aspect ratio of the Arbocel fibers, a suitable Bader and Boyer model variation was adopted in order to have a better quantitative estimation of the IFSS value.
33202908	1	23	contain	containing	260:269	arg1	composites					249:258	poly (lactic) acid (PLA)-based composites	218:258	poly (lactic) acid (PLA)-based composites containing different amounts (from 10 wt	218:299	In this paper, the production and the characterization of poly (lactic) acid (PLA)-based composites containing different amounts (from 10 wt.
33202908	1	23	contain	containing	260:269	arg2	amounts					281:287	different amounts	271:287	different amounts (from 10 wt	271:299	In this paper, the production and the characterization of poly (lactic) acid (PLA)-based composites containing different amounts (from 10 wt.
33202908	9	24	theme	Arbocel	1230:1236	arg1	fibers					1238:1243	the Arbocel fibers	1226:1243	the Arbocel fibers	1226:1243	Furthermore, due to the very low aspect ratio of the Arbocel fibers, a suitable Bader and Boyer model variation was adopted in order to have a better quantitative estimation of the IFSS value.
33202908	1	25	from	wt	298:299	arg1	amounts					281:287	different amounts	271:287	different amounts (from 10 wt	271:299	In this paper, the production and the characterization of poly (lactic) acid (PLA)-based composites containing different amounts (from 10 wt.
33202908	6	26	theme	fiber	797:801	arg1	dispersion					803:812	a better fiber dispersion	788:812	a better fiber dispersion	788:812	The role of the adhesion between the fiber and the matrix, coupled with a better fiber dispersion, was thus evaluated.
33202908	0	27	theme	Interfacial	99:109	arg1	Stress					117:122	the Interfacial Shear Stress	95:122	the Interfacial Shear Stress	95:122	Effect of a Bio-Based Dispersing Aid (Einar® 101) on PLA-Arbocel® Biocomposites: Evaluation of the Interfacial Shear Stress on the Final Mechanical Properties.
33202908	8	28	theme	shear	1072:1076	arg1	IFSS					1086:1089	IFSS	1086:1089	IFSS	1086:1089	Analytical models were also applied in order to obtain an evaluation of the variation of the interfacial shear stress (IFSS) (strictly correlated to the fiber-matrix adhesion) caused by the Einar introduction.
33202908	8	28	theme	shear	1072:1076	arg1	stress					1078:1083	the interfacial shear stress	1056:1083	the interfacial shear stress (IFSS) (strictly correlated to the fiber-matrix adhesion) caused by the Einar introduction	1056:1174	Analytical models were also applied in order to obtain an evaluation of the variation of the interfacial shear stress (IFSS) (strictly correlated to the fiber-matrix adhesion) caused by the Einar introduction.
33202908	9	29	theme	quantitative	1327:1338	arg1	estimation					1340:1349	a better quantitative estimation	1318:1349	a better quantitative estimation of the IFSS value	1318:1367	Furthermore, due to the very low aspect ratio of the Arbocel fibers, a suitable Bader and Boyer model variation was adopted in order to have a better quantitative estimation of the IFSS value.
33202908	6	30	theme	better	790:795	arg1	dispersion					803:812	a better fiber dispersion	788:812	a better fiber dispersion	788:812	The role of the adhesion between the fiber and the matrix, coupled with a better fiber dispersion, was thus evaluated.
33202908	5	31	theme	enhanced	575:582	arg1	resistance					591:600	enhanced impact resistance	575:600	enhanced impact resistance	575:600	Consequently, in order to obtain a composite with enhanced impact resistance and elongation at break, the effect of the Einar 101 addition (a bio-based dispersing aid additive) was analyzed.
33202908	8	32	theme	interfacial	1060:1070	arg1	IFSS					1086:1089	IFSS	1086:1089	IFSS	1086:1089	Analytical models were also applied in order to obtain an evaluation of the variation of the interfacial shear stress (IFSS) (strictly correlated to the fiber-matrix adhesion) caused by the Einar introduction.
33202908	8	32	theme	interfacial	1060:1070	arg1	stress					1078:1083	the interfacial shear stress	1056:1083	the interfacial shear stress (IFSS) (strictly correlated to the fiber-matrix adhesion) caused by the Einar introduction	1056:1174	Analytical models were also applied in order to obtain an evaluation of the variation of the interfacial shear stress (IFSS) (strictly correlated to the fiber-matrix adhesion) caused by the Einar introduction.
33202908	3	33	theme	ultra-short	318:328	arg1	fibers					340:345	ultra-short cellulose fibers	318:345	ultra-short cellulose fibers (Arbocel 600 BE/PU)	318:365	%) of ultra-short cellulose fibers (Arbocel 600 BE/PU) have been investigated.
33202908	3	33	theme	ultra-short	318:328	arg1	BE/PU					360:364	BE/PU	360:364	BE/PU	360:364	%) of ultra-short cellulose fibers (Arbocel 600 BE/PU) have been investigated.
33202908	9	34	theme	better	1320:1325	arg1	estimation					1340:1349	a better quantitative estimation	1318:1349	a better quantitative estimation of the IFSS value	1318:1367	Furthermore, due to the very low aspect ratio of the Arbocel fibers, a suitable Bader and Boyer model variation was adopted in order to have a better quantitative estimation of the IFSS value.
33202908	0	35	theme	Stress	117:122	arg1	Evaluation					81:90	Evaluation	81:90	Effect of a Bio-Based Dispersing Aid (Einar® 101) on PLA-Arbocel® Biocomposites: Evaluation of the Interfacial Shear Stress on the Final Mechanical Properties.	0:158	Effect of a Bio-Based Dispersing Aid (Einar® 101) on PLA-Arbocel® Biocomposites: Evaluation of the Interfacial Shear Stress on the Final Mechanical Properties.
33202908	3	36	theme	cellulose	330:338	arg1	fibers					340:345	ultra-short cellulose fibers	318:345	ultra-short cellulose fibers (Arbocel 600 BE/PU)	318:365	%) of ultra-short cellulose fibers (Arbocel 600 BE/PU) have been investigated.
33202908	3	36	theme	cellulose	330:338	arg1	BE/PU					360:364	BE/PU	360:364	BE/PU	360:364	%) of ultra-short cellulose fibers (Arbocel 600 BE/PU) have been investigated.
33202908	1	37	theme	-based	242:247	arg1	composites					249:258	poly (lactic) acid (PLA)-based composites	218:258	poly (lactic) acid (PLA)-based composites containing different amounts (from 10 wt	218:299	In this paper, the production and the characterization of poly (lactic) acid (PLA)-based composites containing different amounts (from 10 wt.
33202908	0	38	theme	Shear	111:115	arg1	Stress					117:122	the Interfacial Shear Stress	95:122	the Interfacial Shear Stress	95:122	Effect of a Bio-Based Dispersing Aid (Einar® 101) on PLA-Arbocel® Biocomposites: Evaluation of the Interfacial Shear Stress on the Final Mechanical Properties.
33202908	0	39	theme	Bio-Based	12:20	arg1	Effect					0:5	Effect	0:5	Effect of a Bio-Based Dispersing Aid (Einar® 101) on PLA-Arbocel® Biocomposites: Evaluation of the Interfacial Shear Stress on the Final Mechanical Properties.	0:158	Effect of a Bio-Based Dispersing Aid (Einar® 101) on PLA-Arbocel® Biocomposites: Evaluation of the Interfacial Shear Stress on the Final Mechanical Properties.
33202908	4	40	theme	composite	514:522	arg1	embrittlement					493:505	an embrittlement	490:505	an embrittlement of the composite	490:522	On the basis of a previous study, it was observed that the addition of the cellulose fibers led to an embrittlement of the composite.
33202908	1	41	theme	composites	249:258	arg1	characterization					198:213	the characterization	194:213	the characterization of poly (lactic) acid (PLA)-based composites containing different amounts (from 10 wt	194:299	In this paper, the production and the characterization of poly (lactic) acid (PLA)-based composites containing different amounts (from 10 wt.
33202908	1	41	theme	composites	249:258	arg1	production					179:188	the production	175:188	the production	175:188	In this paper, the production and the characterization of poly (lactic) acid (PLA)-based composites containing different amounts (from 10 wt.
33202908	7	42	dep	properties	882:891	arg1	test					913:916	impact test	906:916	impact test	906:916	Also, the consequences on the final mechanical properties (tensile and impact test) caused by the Einar addition were investigated.
33202908	7	42	dep	properties	882:891	arg1	properties					882:891	the final mechanical properties	861:891	the final mechanical properties (tensile and impact test) caused by the Einar addition	861:946	Also, the consequences on the final mechanical properties (tensile and impact test) caused by the Einar addition were investigated.
33202908	7	42	dep	properties	882:891	arg1	tensile					894:900	tensile	894:900	tensile	894:900	Also, the consequences on the final mechanical properties (tensile and impact test) caused by the Einar addition were investigated.
33202908	9	43	theme	value	1363:1367	arg1	estimation					1340:1349	a better quantitative estimation	1318:1349	a better quantitative estimation of the IFSS value	1318:1367	Furthermore, due to the very low aspect ratio of the Arbocel fibers, a suitable Bader and Boyer model variation was adopted in order to have a better quantitative estimation of the IFSS value.
33202908	9	44	theme	Bader	1257:1261	arg1	variation					1279:1287	a suitable Bader and Boyer model variation	1246:1287	a suitable Bader and Boyer model variation	1246:1287	Furthermore, due to the very low aspect ratio of the Arbocel fibers, a suitable Bader and Boyer model variation was adopted in order to have a better quantitative estimation of the IFSS value.
33202908	4	45	dep	study	418:422	arg1	the					394:396	the	394:396	the	394:396	On the basis of a previous study, it was observed that the addition of the cellulose fibers led to an embrittlement of the composite.
33202908	4	45	dep	study	418:422	arg1	basis					398:402	basis	398:402	basis	398:402	On the basis of a previous study, it was observed that the addition of the cellulose fibers led to an embrittlement of the composite.
33202908	7	46	theme	final	865:869	arg1	test					913:916	impact test	906:916	impact test	906:916	Also, the consequences on the final mechanical properties (tensile and impact test) caused by the Einar addition were investigated.
33202908	7	46	theme	final	865:869	arg1	properties					882:891	the final mechanical properties	861:891	the final mechanical properties (tensile and impact test) caused by the Einar addition	861:946	Also, the consequences on the final mechanical properties (tensile and impact test) caused by the Einar addition were investigated.
33202908	7	46	theme	final	865:869	arg1	tensile					894:900	tensile	894:900	tensile	894:900	Also, the consequences on the final mechanical properties (tensile and impact test) caused by the Einar addition were investigated.
33202908	6	47	theme	fiber	753:757	arg1	matrix					767:772	the fiber and the matrix	749:772	matrix	767:772	The role of the adhesion between the fiber and the matrix, coupled with a better fiber dispersion, was thus evaluated.
33202908	8	48	dep	correlated	1102:1111	arg1	strictly					1093:1100	strictly	1093:1100	strictly	1093:1100	Analytical models were also applied in order to obtain an evaluation of the variation of the interfacial shear stress (IFSS) (strictly correlated to the fiber-matrix adhesion) caused by the Einar introduction.
33202908	1	49	theme	different	271:279	arg1	amounts					281:287	different amounts	271:287	different amounts (from 10 wt	271:299	In this paper, the production and the characterization of poly (lactic) acid (PLA)-based composites containing different amounts (from 10 wt.
33202908	0	50	theme	Mechanical	137:146	arg1	Properties					148:157	the Final Mechanical Properties	127:157	the Final Mechanical Properties	127:157	Effect of a Bio-Based Dispersing Aid (Einar® 101) on PLA-Arbocel® Biocomposites: Evaluation of the Interfacial Shear Stress on the Final Mechanical Properties.
33202908	0	51	from	Evaluation	81:90	arg1	Properties					148:157	the Final Mechanical Properties	127:157	the Final Mechanical Properties	127:157	Effect of a Bio-Based Dispersing Aid (Einar® 101) on PLA-Arbocel® Biocomposites: Evaluation of the Interfacial Shear Stress on the Final Mechanical Properties.
33202908	0	52	dep	Bio-Based	12:20	arg1	Aid					33:35	Aid	33:35	Aid	33:35	Effect of a Bio-Based Dispersing Aid (Einar® 101) on PLA-Arbocel® Biocomposites: Evaluation of the Interfacial Shear Stress on the Final Mechanical Properties.
33202908	9	53	theme	IFSS	1358:1361	arg1	value					1363:1367	the IFSS value	1354:1367	the IFSS value	1354:1367	Furthermore, due to the very low aspect ratio of the Arbocel fibers, a suitable Bader and Boyer model variation was adopted in order to have a better quantitative estimation of the IFSS value.
33202908	9	54	theme	Boyer	1267:1271	arg1	variation					1279:1287	a suitable Bader and Boyer model variation	1246:1287	a suitable Bader and Boyer model variation	1246:1287	Furthermore, due to the very low aspect ratio of the Arbocel fibers, a suitable Bader and Boyer model variation was adopted in order to have a better quantitative estimation of the IFSS value.
33202908	7	55	from	consequences	845:856	arg1	test					913:916	impact test	906:916	impact test	906:916	Also, the consequences on the final mechanical properties (tensile and impact test) caused by the Einar addition were investigated.
33202908	7	55	from	consequences	845:856	arg1	properties					882:891	the final mechanical properties	861:891	the final mechanical properties (tensile and impact test) caused by the Einar addition	861:946	Also, the consequences on the final mechanical properties (tensile and impact test) caused by the Einar addition were investigated.
33202908	7	55	from	consequences	845:856	arg1	tensile					894:900	tensile	894:900	tensile	894:900	Also, the consequences on the final mechanical properties (tensile and impact test) caused by the Einar addition were investigated.
33202908	0	56	theme	Final	131:135	arg1	Properties					148:157	the Final Mechanical Properties	127:157	the Final Mechanical Properties	127:157	Effect of a Bio-Based Dispersing Aid (Einar® 101) on PLA-Arbocel® Biocomposites: Evaluation of the Interfacial Shear Stress on the Final Mechanical Properties.
33202908	1	57	dep	production	179:188	arg1	paper					168:172	this paper	163:172	this paper	163:172	In this paper, the production and the characterization of poly (lactic) acid (PLA)-based composites containing different amounts (from 10 wt.
33202908	9	58	theme	model	1273:1277	arg1	variation					1279:1287	a suitable Bader and Boyer model variation	1246:1287	a suitable Bader and Boyer model variation	1246:1287	Furthermore, due to the very low aspect ratio of the Arbocel fibers, a suitable Bader and Boyer model variation was adopted in order to have a better quantitative estimation of the IFSS value.
33202908	7	59	theme	impact	906:911	arg1	test					913:916	impact test	906:916	impact test	906:916	Also, the consequences on the final mechanical properties (tensile and impact test) caused by the Einar addition were investigated.
33202908	7	59	theme	impact	906:911	arg1	properties					882:891	the final mechanical properties	861:891	the final mechanical properties (tensile and impact test) caused by the Einar addition	861:946	Also, the consequences on the final mechanical properties (tensile and impact test) caused by the Einar addition were investigated.
33202908	9	60	theme	suitable	1248:1255	arg1	variation					1279:1287	a suitable Bader and Boyer model variation	1246:1287	a suitable Bader and Boyer model variation	1246:1287	Furthermore, due to the very low aspect ratio of the Arbocel fibers, a suitable Bader and Boyer model variation was adopted in order to have a better quantitative estimation of the IFSS value.
33202908	8	61	theme	stress	1078:1083	arg1	variation					1043:1051	the variation	1039:1051	the variation of the interfacial shear stress (IFSS) (strictly correlated to the fiber-matrix adhesion) caused by the Einar introduction	1039:1174	Analytical models were also applied in order to obtain an evaluation of the variation of the interfacial shear stress (IFSS) (strictly correlated to the fiber-matrix adhesion) caused by the Einar introduction.
33202908	9	62	theme	fibers	1238:1243	arg1	ratio					1217:1221	the very low aspect ratio	1197:1221	the very low aspect ratio of the Arbocel fibers	1197:1243	Furthermore, due to the very low aspect ratio of the Arbocel fibers, a suitable Bader and Boyer model variation was adopted in order to have a better quantitative estimation of the IFSS value.
33202908	4	63	theme	fibers	476:481	arg1	addition					450:457	the addition	446:457	the addition of the cellulose fibers	446:481	On the basis of a previous study, it was observed that the addition of the cellulose fibers led to an embrittlement of the composite.
33202908	6	64	theme	the	763:765	arg1	matrix					767:772	the fiber and the matrix	749:772	matrix	767:772	The role of the adhesion between the fiber and the matrix, coupled with a better fiber dispersion, was thus evaluated.
33202908	0	65	dep	Effect	0:5	arg1	Evaluation					81:90	Evaluation	81:90	Effect of a Bio-Based Dispersing Aid (Einar® 101) on PLA-Arbocel® Biocomposites: Evaluation of the Interfacial Shear Stress on the Final Mechanical Properties.	0:158	Effect of a Bio-Based Dispersing Aid (Einar® 101) on PLA-Arbocel® Biocomposites: Evaluation of the Interfacial Shear Stress on the Final Mechanical Properties.
33202908	8	66	theme	variation	1043:1051	arg1	evaluation					1025:1034	an evaluation	1022:1034	an evaluation of the variation of the interfacial shear stress (IFSS) (strictly correlated to the fiber-matrix adhesion) caused by the Einar introduction	1022:1174	Analytical models were also applied in order to obtain an evaluation of the variation of the interfacial shear stress (IFSS) (strictly correlated to the fiber-matrix adhesion) caused by the Einar introduction.
31923499	0	0	theme	imidacloprid	99:110	arg1	insecticide					112:122	imidacloprid insecticide	99:122	imidacloprid insecticide	99:122	Polypyrole, polyaniline and sodium alginate biocomposites and adsorption-desorption efficiency for imidacloprid insecticide.
31923499	4	1	theme	90 min	683:688	arg1	time					698:701	90 min contact time	683:701	90 min contact time	683:701	Among all composites polypyrole composite exhibited maximum efficiency at pH 3, 0.05 g adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models were applied on the experimental data.
31923499	4	2	theme	time	698:701	arg1	models					800:805	adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models	667:805	adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models	667:805	Among all composites polypyrole composite exhibited maximum efficiency at pH 3, 0.05 g adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models were applied on the experimental data.
31923499	8	3	with	composites	1260:1269	arg1	husk					1283:1286	peanut husk	1276:1286	peanut husk	1276:1286	This study revealed that the polypyrole, polyaniline and sodium alginate composites with peanut husk have promising adsorption potential and this class of adsorbent could be used for the remediation of wastewater contains imidacloprid insecticide.
31923499	4	4	theme	contact	690:696	arg1	time					698:701	90 min contact time	683:701	90 min contact time	683:701	Among all composites polypyrole composite exhibited maximum efficiency at pH 3, 0.05 g adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models were applied on the experimental data.
31923499	1	5	theme	polyaniline	267:277	arg1	polyaniline					267:277	polyaniline	267:277	polyaniline (PAN)	267:283	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	1	5	theme	polyaniline	267:277	arg1	polypyrole					249:258	polypyrole	249:258	polypyrole (PPY)	249:264	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	1	5	theme	polyaniline	267:277	arg1	alginate					296:303	sodium alginate	289:303	sodium alginate (Na-Alginate)	289:317	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	1	5	theme	polyaniline	267:277	arg1	biocomposites					232:244	biocomposites	232:244	biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH)	232:339	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	4	6	theme	experimental	827:838	arg1	data					840:843	the experimental data	823:843	the experimental data	823:843	Among all composites polypyrole composite exhibited maximum efficiency at pH 3, 0.05 g adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models were applied on the experimental data.
31923499	3	7	theme	promising	526:534	arg1	efficiency					536:545	promising efficiency	526:545	promising efficiency for the removal of imidacloprid	526:577	The biocomposites showed promising efficiency for the removal of imidacloprid.
31923499	6	8	theme	kinetics	1003:1010	arg1	model					1012:1016	the pseudo first order kinetics model	980:1016	the pseudo first order kinetics model	980:1016	Insecticide adsorption followed the pseudo first order kinetics model.
31923499	7	9	from	nature	1179:1184	arg1	spontaneous					1149:1159	spontaneous	1149:1159	spontaneous	1149:1159	Thermodynamics (free energy, enthalpy and entropy) study revealed that the insecticide adsorption process on to biocomposites was spontaneous and exothermic in nature.
31923499	7	9	from	nature	1179:1184	arg1	process					1117:1123	the insecticide adsorption process	1090:1123	the insecticide adsorption process on to biocomposites	1090:1143	Thermodynamics (free energy, enthalpy and entropy) study revealed that the insecticide adsorption process on to biocomposites was spontaneous and exothermic in nature.
31923499	5	10	with	isotherms	891:899	arg1	>0.97					927:931	>0.97	927:931	>0.97	927:931	The best fitted were Langmuir and Freundlich isotherms with R2 value > 0.904 and >0.97, respectively.
31923499	5	10	with	isotherms	891:899	arg1	value > 0.904					909:921	R2 value > 0.904	906:921	R2 value > 0.904	906:921	The best fitted were Langmuir and Freundlich isotherms with R2 value > 0.904 and >0.97, respectively.
31923499	7	11	dep	Thermodynamics	1019:1032	arg1	entropy					1061:1067	entropy	1061:1067	entropy	1061:1067	Thermodynamics (free energy, enthalpy and entropy) study revealed that the insecticide adsorption process on to biocomposites was spontaneous and exothermic in nature.
31923499	7	11	dep	Thermodynamics	1019:1032	arg1	enthalpy					1048:1055	enthalpy	1048:1055	enthalpy	1048:1055	Thermodynamics (free energy, enthalpy and entropy) study revealed that the insecticide adsorption process on to biocomposites was spontaneous and exothermic in nature.
31923499	7	11	dep	Thermodynamics	1019:1032	arg1	energy					1040:1045	free energy	1035:1045	free energy	1035:1045	Thermodynamics (free energy, enthalpy and entropy) study revealed that the insecticide adsorption process on to biocomposites was spontaneous and exothermic in nature.
31923499	4	12	theme	initial	712:718	arg1	concentration					720:732	25 mg/L initial concentration	704:732	25 mg/L initial concentration at 35 °C. Langmuir	704:751	Among all composites polypyrole composite exhibited maximum efficiency at pH 3, 0.05 g adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models were applied on the experimental data.
31923499	2	13	theme	imidacloprid	487:498	arg1	removal					476:482	efficient removal	466:482	efficient removal of imidacloprid	466:498	The affecting variables such as pH, insecticide concentration, composite dose, contact time, temperature were optimized for efficient removal of imidacloprid.
31923499	7	14	theme	adsorption	1106:1115	arg1	spontaneous					1149:1159	spontaneous	1149:1159	spontaneous	1149:1159	Thermodynamics (free energy, enthalpy and entropy) study revealed that the insecticide adsorption process on to biocomposites was spontaneous and exothermic in nature.
31923499	7	14	theme	adsorption	1106:1115	arg1	process					1117:1123	the insecticide adsorption process	1090:1123	the insecticide adsorption process on to biocomposites	1090:1143	Thermodynamics (free energy, enthalpy and entropy) study revealed that the insecticide adsorption process on to biocomposites was spontaneous and exothermic in nature.
31923499	4	15	theme	dose	677:680	arg1	models					800:805	adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models	667:805	adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models	667:805	Among all composites polypyrole composite exhibited maximum efficiency at pH 3, 0.05 g adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models were applied on the experimental data.
31923499	6	16	theme	order	997:1001	arg1	model					1012:1016	the pseudo first order kinetics model	980:1016	the pseudo first order kinetics model	980:1016	Insecticide adsorption followed the pseudo first order kinetics model.
31923499	8	17	theme	polyaniline	1228:1238	arg1	composites					1260:1269	the polypyrole, polyaniline and sodium alginate composites	1212:1269	the polypyrole, polyaniline and sodium alginate composites with peanut husk	1212:1286	This study revealed that the polypyrole, polyaniline and sodium alginate composites with peanut husk have promising adsorption potential and this class of adsorbent could be used for the remediation of wastewater contains imidacloprid insecticide.
31923499	4	18	theme	Harkins-Jura	766:777	arg1	models					800:805	adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models	667:805	adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models	667:805	Among all composites polypyrole composite exhibited maximum efficiency at pH 3, 0.05 g adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models were applied on the experimental data.
31923499	8	19	theme	alginate	1251:1258	arg1	composites					1260:1269	the polypyrole, polyaniline and sodium alginate composites	1212:1269	the polypyrole, polyaniline and sodium alginate composites with peanut husk	1212:1286	This study revealed that the polypyrole, polyaniline and sodium alginate composites with peanut husk have promising adsorption potential and this class of adsorbent could be used for the remediation of wastewater contains imidacloprid insecticide.
31923499	2	20	theme	contact	421:427	arg1	pH					374:375	pH	374:375	pH	374:375	The affecting variables such as pH, insecticide concentration, composite dose, contact time, temperature were optimized for efficient removal of imidacloprid.
31923499	2	20	theme	contact	421:427	arg1	time					429:432	contact time	421:432	contact time	421:432	The affecting variables such as pH, insecticide concentration, composite dose, contact time, temperature were optimized for efficient removal of imidacloprid.
31923499	4	21	from	Langmuir	744:751	arg1	concentration					720:732	25 mg/L initial concentration	704:732	25 mg/L initial concentration at 35 °C. Langmuir	704:751	Among all composites polypyrole composite exhibited maximum efficiency at pH 3, 0.05 g adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models were applied on the experimental data.
31923499	4	21	from	Langmuir	744:751	arg1	time					698:701	90 min contact time	683:701	90 min contact time	683:701	Among all composites polypyrole composite exhibited maximum efficiency at pH 3, 0.05 g adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models were applied on the experimental data.
31923499	4	21	from	Langmuir	744:751	arg1	isotherms					790:798	Temkin isotherms	783:798	Temkin isotherms	783:798	Among all composites polypyrole composite exhibited maximum efficiency at pH 3, 0.05 g adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models were applied on the experimental data.
31923499	4	21	from	Langmuir	744:751	arg1	dose					677:680	dose	677:680	dose	677:680	Among all composites polypyrole composite exhibited maximum efficiency at pH 3, 0.05 g adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models were applied on the experimental data.
31923499	4	22	theme	35 °C.	737:742	arg1	Langmuir					744:751	35 °C. Langmuir	737:751	35 °C. Langmuir	737:751	Among all composites polypyrole composite exhibited maximum efficiency at pH 3, 0.05 g adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models were applied on the experimental data.
31923499	1	23	theme	imidacloprid	201:212	arg1	removal					190:196	the removal	186:196	the removal of imidacloprid	186:212	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	0	24	theme	Polypyrole	0:9	arg1	biocomposites					44:56	Polypyrole, polyaniline and sodium alginate biocomposites	0:56	Polypyrole, polyaniline and sodium alginate biocomposites	0:56	Polypyrole, polyaniline and sodium alginate biocomposites and adsorption-desorption efficiency for imidacloprid insecticide.
31923499	6	25	theme	first	991:995	arg1	model					1012:1016	the pseudo first order kinetics model	980:1016	the pseudo first order kinetics model	980:1016	Insecticide adsorption followed the pseudo first order kinetics model.
31923499	7	26	theme	insecticide	1094:1104	arg1	spontaneous					1149:1159	spontaneous	1149:1159	spontaneous	1149:1159	Thermodynamics (free energy, enthalpy and entropy) study revealed that the insecticide adsorption process on to biocomposites was spontaneous and exothermic in nature.
31923499	7	26	theme	insecticide	1094:1104	arg1	process					1117:1123	the insecticide adsorption process	1090:1123	the insecticide adsorption process on to biocomposites	1090:1143	Thermodynamics (free energy, enthalpy and entropy) study revealed that the insecticide adsorption process on to biocomposites was spontaneous and exothermic in nature.
31923499	4	27	theme	adsorbent	667:675	arg1	models					800:805	adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models	667:805	adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models	667:805	Among all composites polypyrole composite exhibited maximum efficiency at pH 3, 0.05 g adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models were applied on the experimental data.
31923499	8	28	theme	polypyrole	1216:1225	arg1	composites					1260:1269	the polypyrole, polyaniline and sodium alginate composites	1212:1269	the polypyrole, polyaniline and sodium alginate composites with peanut husk	1212:1286	This study revealed that the polypyrole, polyaniline and sodium alginate composites with peanut husk have promising adsorption potential and this class of adsorbent could be used for the remediation of wastewater contains imidacloprid insecticide.
31923499	6	29	theme	pseudo	984:989	arg1	model					1012:1016	the pseudo first order kinetics model	980:1016	the pseudo first order kinetics model	980:1016	Insecticide adsorption followed the pseudo first order kinetics model.
31923499	1	30	with	biocomposites	232:244	arg1	PH					337:338	PH	337:338	PH	337:338	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	1	30	with	biocomposites	232:244	arg1	husk					331:334	peanut husk	324:334	peanut husk (PH)	324:339	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	8	31	theme	imidacloprid	1409:1420	arg1	insecticide					1422:1432	imidacloprid insecticide	1409:1432	imidacloprid insecticide	1409:1432	This study revealed that the polypyrole, polyaniline and sodium alginate composites with peanut husk have promising adsorption potential and this class of adsorbent could be used for the remediation of wastewater contains imidacloprid insecticide.
31923499	2	32	theme	composite	405:413	arg1	pH					374:375	pH	374:375	pH	374:375	The affecting variables such as pH, insecticide concentration, composite dose, contact time, temperature were optimized for efficient removal of imidacloprid.
31923499	2	32	theme	composite	405:413	arg1	dose					415:418	composite dose	405:418	composite dose	405:418	The affecting variables such as pH, insecticide concentration, composite dose, contact time, temperature were optimized for efficient removal of imidacloprid.
31923499	1	33	theme	sodium	289:294	arg1	Na-Alginate					306:316	Na-Alginate	306:316	Na-Alginate	306:316	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	1	33	theme	sodium	289:294	arg1	alginate					296:303	sodium alginate	289:303	sodium alginate (Na-Alginate)	289:317	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	4	34	theme	polypyrole	601:610	arg1	composite					612:620	polypyrole composite	601:620	polypyrole composite	601:620	Among all composites polypyrole composite exhibited maximum efficiency at pH 3, 0.05 g adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models were applied on the experimental data.
31923499	5	35	theme	R2	906:907	arg1	value > 0.904					909:921	R2 value > 0.904	906:921	R2 value > 0.904	906:921	The best fitted were Langmuir and Freundlich isotherms with R2 value > 0.904 and >0.97, respectively.
31923499	4	36	theme	maximum	632:638	arg1	efficiency					640:649	maximum efficiency	632:649	maximum efficiency	632:649	Among all composites polypyrole composite exhibited maximum efficiency at pH 3, 0.05 g adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models were applied on the experimental data.
31923499	0	37	theme	alginate	35:42	arg1	biocomposites					44:56	Polypyrole, polyaniline and sodium alginate biocomposites	0:56	Polypyrole, polyaniline and sodium alginate biocomposites	0:56	Polypyrole, polyaniline and sodium alginate biocomposites and adsorption-desorption efficiency for imidacloprid insecticide.
31923499	1	38	theme	alginate	296:303	arg1	polyaniline					267:277	polyaniline	267:277	polyaniline (PAN)	267:283	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	1	38	theme	alginate	296:303	arg1	polypyrole					249:258	polypyrole	249:258	polypyrole (PPY)	249:264	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	1	38	theme	alginate	296:303	arg1	alginate					296:303	sodium alginate	289:303	sodium alginate (Na-Alginate)	289:317	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	1	38	theme	alginate	296:303	arg1	biocomposites					232:244	biocomposites	232:244	biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH)	232:339	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	8	39	theme	adsorption	1303:1312	arg1	potential					1314:1322	promising adsorption potential	1293:1322	promising adsorption potential	1293:1322	This study revealed that the polypyrole, polyaniline and sodium alginate composites with peanut husk have promising adsorption potential and this class of adsorbent could be used for the remediation of wastewater contains imidacloprid insecticide.
31923499	8	40	theme	adsorbent	1342:1350	arg1	class					1333:1337	this class	1328:1337	this class of adsorbent	1328:1350	This study revealed that the polypyrole, polyaniline and sodium alginate composites with peanut husk have promising adsorption potential and this class of adsorbent could be used for the remediation of wastewater contains imidacloprid insecticide.
31923499	2	41	theme	insecticide	378:388	arg1	pH					374:375	pH	374:375	pH	374:375	The affecting variables such as pH, insecticide concentration, composite dose, contact time, temperature were optimized for efficient removal of imidacloprid.
31923499	2	41	theme	insecticide	378:388	arg1	concentration					390:402	insecticide concentration	378:402	insecticide concentration	378:402	The affecting variables such as pH, insecticide concentration, composite dose, contact time, temperature were optimized for efficient removal of imidacloprid.
31923499	0	42	theme	sodium	28:33	arg1	biocomposites					44:56	Polypyrole, polyaniline and sodium alginate biocomposites	0:56	Polypyrole, polyaniline and sodium alginate biocomposites	0:56	Polypyrole, polyaniline and sodium alginate biocomposites and adsorption-desorption efficiency for imidacloprid insecticide.
31923499	5	43	theme	Langmuir	867:874	arg1	best					850:853	best	850:853	best	850:853	The best fitted were Langmuir and Freundlich isotherms with R2 value > 0.904 and >0.97, respectively.
31923499	5	43	theme	Langmuir	867:874	arg1	isotherms					891:899	Langmuir and Freundlich isotherms	867:899	Langmuir and Freundlich isotherms	867:899	The best fitted were Langmuir and Freundlich isotherms with R2 value > 0.904 and >0.97, respectively.
31923499	8	44	contain	have	1288:1291	arg2	potential					1314:1322	promising adsorption potential	1293:1322	promising adsorption potential	1293:1322	This study revealed that the polypyrole, polyaniline and sodium alginate composites with peanut husk have promising adsorption potential and this class of adsorbent could be used for the remediation of wastewater contains imidacloprid insecticide.
31923499	8	44	contain	have	1288:1291	arg1	composites					1260:1269	the polypyrole, polyaniline and sodium alginate composites	1212:1269	the polypyrole, polyaniline and sodium alginate composites with peanut husk	1212:1286	This study revealed that the polypyrole, polyaniline and sodium alginate composites with peanut husk have promising adsorption potential and this class of adsorbent could be used for the remediation of wastewater contains imidacloprid insecticide.
31923499	7	45	theme	free	1035:1038	arg1	energy					1040:1045	free energy	1035:1045	free energy	1035:1045	Thermodynamics (free energy, enthalpy and entropy) study revealed that the insecticide adsorption process on to biocomposites was spontaneous and exothermic in nature.
31923499	8	46	theme	promising	1293:1301	arg1	potential					1314:1322	promising adsorption potential	1293:1322	promising adsorption potential	1293:1322	This study revealed that the polypyrole, polyaniline and sodium alginate composites with peanut husk have promising adsorption potential and this class of adsorbent could be used for the remediation of wastewater contains imidacloprid insecticide.
31923499	8	47	theme	sodium	1244:1249	arg1	alginate					1251:1258	sodium alginate	1244:1258	sodium alginate	1244:1258	This study revealed that the polypyrole, polyaniline and sodium alginate composites with peanut husk have promising adsorption potential and this class of adsorbent could be used for the remediation of wastewater contains imidacloprid insecticide.
31923499	5	48	theme	Freundlich	880:889	arg1	best					850:853	best	850:853	best	850:853	The best fitted were Langmuir and Freundlich isotherms with R2 value > 0.904 and >0.97, respectively.
31923499	5	48	theme	Freundlich	880:889	arg1	isotherms					891:899	Langmuir and Freundlich isotherms	867:899	Langmuir and Freundlich isotherms	867:899	The best fitted were Langmuir and Freundlich isotherms with R2 value > 0.904 and >0.97, respectively.
31923499	0	49	theme	adsorption-desorption	62:82	arg1	efficiency					84:93	adsorption-desorption efficiency	62:93	adsorption-desorption efficiency	62:93	Polypyrole, polyaniline and sodium alginate biocomposites and adsorption-desorption efficiency for imidacloprid insecticide.
31923499	3	50	theme	imidacloprid	566:577	arg1	removal					555:561	the removal	551:561	the removal of imidacloprid	551:577	The biocomposites showed promising efficiency for the removal of imidacloprid.
31923499	4	51	theme	25 mg/L	704:710	arg1	concentration					720:732	25 mg/L initial concentration	704:732	25 mg/L initial concentration at 35 °C. Langmuir	704:751	Among all composites polypyrole composite exhibited maximum efficiency at pH 3, 0.05 g adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models were applied on the experimental data.
31923499	7	52	from	spontaneous	1149:1159	arg1	nature					1179:1184	nature	1179:1184	nature	1179:1184	Thermodynamics (free energy, enthalpy and entropy) study revealed that the insecticide adsorption process on to biocomposites was spontaneous and exothermic in nature.
31923499	7	53	theme	Thermodynamics	1019:1032	arg1	study					1070:1074	Thermodynamics (free energy, enthalpy and entropy) study	1019:1074	Thermodynamics (free energy, enthalpy and entropy) study	1019:1074	Thermodynamics (free energy, enthalpy and entropy) study revealed that the insecticide adsorption process on to biocomposites was spontaneous and exothermic in nature.
31923499	1	54	theme	promising	136:144	arg1	efficiency					157:166	promising adsorption efficiency	136:166	promising adsorption efficiency of biocomposites	136:183	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	4	55	theme	isotherms	790:798	arg1	models					800:805	adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models	667:805	adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models	667:805	Among all composites polypyrole composite exhibited maximum efficiency at pH 3, 0.05 g adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models were applied on the experimental data.
31923499	8	56	theme	peanut	1276:1281	arg1	husk					1283:1286	peanut husk	1276:1286	peanut husk	1276:1286	This study revealed that the polypyrole, polyaniline and sodium alginate composites with peanut husk have promising adsorption potential and this class of adsorbent could be used for the remediation of wastewater contains imidacloprid insecticide.
31923499	1	57	theme	adsorption	146:155	arg1	efficiency					157:166	promising adsorption efficiency	136:166	promising adsorption efficiency of biocomposites	136:183	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	1	58	theme	polypyrole	249:258	arg1	polyaniline					267:277	polyaniline	267:277	polyaniline (PAN)	267:283	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	1	58	theme	polypyrole	249:258	arg1	polypyrole					249:258	polypyrole	249:258	polypyrole (PPY)	249:264	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	1	58	theme	polypyrole	249:258	arg1	alginate					296:303	sodium alginate	289:303	sodium alginate (Na-Alginate)	289:317	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	1	58	theme	polypyrole	249:258	arg1	biocomposites					232:244	biocomposites	232:244	biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH)	232:339	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	8	59	used	used	1361:1364	arg2	class					1333:1337	this class	1328:1337	this class of adsorbent	1328:1350	This study revealed that the polypyrole, polyaniline and sodium alginate composites with peanut husk have promising adsorption potential and this class of adsorbent could be used for the remediation of wastewater contains imidacloprid insecticide.
31923499	4	60	theme	Temkin	783:788	arg1	isotherms					790:798	Temkin isotherms	783:798	Temkin isotherms	783:798	Among all composites polypyrole composite exhibited maximum efficiency at pH 3, 0.05 g adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models were applied on the experimental data.
31923499	1	61	theme	efficiency	157:166	arg1	view					128:131	view	128:131	view of promising adsorption efficiency of biocomposites	128:183	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	4	62	theme	concentration	720:732	arg1	models					800:805	adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models	667:805	adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models	667:805	Among all composites polypyrole composite exhibited maximum efficiency at pH 3, 0.05 g adsorbent dose, 90 min contact time, 25 mg/L initial concentration at 35 °C. Langmuir, Freundlich, Harkins-Jura and Temkin isotherms models were applied on the experimental data.
31923499	8	63	contain	contains	1400:1407	arg1	wastewater					1389:1398	wastewater	1389:1398	wastewater	1389:1398	This study revealed that the polypyrole, polyaniline and sodium alginate composites with peanut husk have promising adsorption potential and this class of adsorbent could be used for the remediation of wastewater contains imidacloprid insecticide.
31923499	8	63	contain	contains	1400:1407	arg2	insecticide					1422:1432	imidacloprid insecticide	1409:1432	imidacloprid insecticide	1409:1432	This study revealed that the polypyrole, polyaniline and sodium alginate composites with peanut husk have promising adsorption potential and this class of adsorbent could be used for the remediation of wastewater contains imidacloprid insecticide.
31923499	2	64	theme	affecting	346:354	arg1	variables					356:364	The affecting variables	342:364	The affecting variables such as pH, insecticide concentration, composite dose, contact time, temperature	342:445	The affecting variables such as pH, insecticide concentration, composite dose, contact time, temperature were optimized for efficient removal of imidacloprid.
31923499	2	64	theme	affecting	346:354	arg1	pH					374:375	pH	374:375	pH	374:375	The affecting variables such as pH, insecticide concentration, composite dose, contact time, temperature were optimized for efficient removal of imidacloprid.
31923499	2	65	theme	efficient	466:474	arg1	removal					476:482	efficient removal	466:482	efficient removal of imidacloprid	466:498	The affecting variables such as pH, insecticide concentration, composite dose, contact time, temperature were optimized for efficient removal of imidacloprid.
31923499	1	66	theme	peanut	324:329	arg1	PH					337:338	PH	337:338	PH	337:338	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	1	66	theme	peanut	324:329	arg1	husk					331:334	peanut husk	324:334	peanut husk (PH)	324:339	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	0	67	theme	polyaniline	12:22	arg1	biocomposites					44:56	Polypyrole, polyaniline and sodium alginate biocomposites	0:56	Polypyrole, polyaniline and sodium alginate biocomposites	0:56	Polypyrole, polyaniline and sodium alginate biocomposites and adsorption-desorption efficiency for imidacloprid insecticide.
31923499	1	68	theme	biocomposites	171:183	arg1	efficiency					157:166	promising adsorption efficiency	136:166	promising adsorption efficiency of biocomposites	136:183	In view of promising adsorption efficiency of biocomposites, the removal of imidacloprid was studied using biocomposites of polypyrole (PPY), polyaniline (PAN) and sodium alginate (Na-Alginate) with peanut husk (PH).
31923499	6	69	theme	Insecticide	948:958	arg1	adsorption					960:969	Insecticide adsorption	948:969	Insecticide adsorption	948:969	Insecticide adsorption followed the pseudo first order kinetics model.
34013268	2	0	theme	variety	445:451	arg1	target					433:438	the target	429:438	the target of a variety of neutralizing antibodies	429:478	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 (ACE2) binding and thus is the target of a variety of neutralizing antibodies.
34013268	3	1	theme	all-atom	651:658	arg1	dynamics					670:677	all-atom molecular dynamics	651:677	all-atom molecular dynamics simulations	651:689	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work, and performed all-atom molecular dynamics simulations to get insight into the structural dynamics and interactions between S protein and antibodies.
34013268	7	2	used	used	1339:1342	arg2	maps					1325:1328	the simulation-based S-antibody interaction maps	1281:1328	the simulation-based S-antibody interaction maps	1281:1328	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.
34013268	7	3	theme	vaccine	1456:1462	arg1	development					1441:1451	the development	1437:1451	the development of vaccine and antibody-based therapy	1437:1489	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.
34013268	4	4	theme	S-antibody	829:838	arg1	binding					840:846	S-antibody binding	829:846	S-antibody binding	829:846	Investigation of the residues critical for S-antibody binding allows us to predict the potential influence of mutations in SARS-CoV-2 variants.
34013268	4	5	from	influence	883:891	arg1	variants					920:927	SARS-CoV-2 variants	909:927	SARS-CoV-2 variants	909:927	Investigation of the residues critical for S-antibody binding allows us to predict the potential influence of mutations in SARS-CoV-2 variants.
34013268	7	6	theme	S-antibody	1302:1311	arg1	maps					1325:1328	the simulation-based S-antibody interaction maps	1281:1328	the simulation-based S-antibody interaction maps	1281:1328	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.
34013268	1	7	theme	potent	241:246	arg1	antibodies					261:270	highly potent neutralizing antibodies	234:270	highly potent neutralizing antibodies	234:270	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.
34013268	2	8	from	protein	325:331	arg1	envelope					346:353	the viral envelope	336:353	the viral envelope	336:353	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 (ACE2) binding and thus is the target of a variety of neutralizing antibodies.
34013268	3	9	theme	S	512:512	arg1	structures					538:547	various S trimer-antibody complex structures	504:547	various S trimer-antibody complex structures	504:547	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work, and performed all-atom molecular dynamics simulations to get insight into the structural dynamics and interactions between S protein and antibodies.
34013268	1	10	theme	neutralizing	248:259	arg1	antibodies					261:270	highly potent neutralizing antibodies	234:270	highly potent neutralizing antibodies	234:270	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.
34013268	4	11	theme	SARS-CoV-2	909:918	arg1	variants					920:927	SARS-CoV-2 variants	909:927	SARS-CoV-2 variants	909:927	Investigation of the residues critical for S-antibody binding allows us to predict the potential influence of mutations in SARS-CoV-2 variants.
34013268	6	12	theme	protein	1162:1168	arg1	domains					1187:1193	S protein receptor binding domains	1160:1193	S protein receptor binding domains	1160:1193	In addition, we explored the antibody binding modes, and the influences of antibody on the motion of S protein receptor binding domains.
34013268	2	13	theme	spike	315:319	arg1	protein					325:331	The spike (S) protein	311:331	The spike (S) protein on the viral envelope	311:353	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 (ACE2) binding and thus is the target of a variety of neutralizing antibodies.
34013268	4	14	theme	residues	807:814	arg1	Investigation					786:798	Investigation	786:798	Investigation of the residues critical for S-antibody binding	786:846	Investigation of the residues critical for S-antibody binding allows us to predict the potential influence of mutations in SARS-CoV-2 variants.
34013268	5	15	theme	glycan	948:953	arg1	conformations					955:967	the glycan conformations	944:967	the glycan conformations	944:967	Comparison of the glycan conformations between S-only and S-antibody systems reveals the roles of glycans in S-antibody binding.
34013268	2	16	theme	angiotensin-converting	370:391	arg1	enzyme					393:398	human angiotensin-converting enzyme 2	364:400	human angiotensin-converting enzyme 2 (ACE2) binding	364:415	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 (ACE2) binding and thus is the target of a variety of neutralizing antibodies.
34013268	2	16	theme	angiotensin-converting	370:391	arg1	ACE2					403:406	ACE2	403:406	ACE2	403:406	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 (ACE2) binding and thus is the target of a variety of neutralizing antibodies.
34013268	2	17	theme	antibodies	469:478	arg1	variety					445:451	a variety	443:451	a variety of neutralizing antibodies	443:478	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 (ACE2) binding and thus is the target of a variety of neutralizing antibodies.
34013268	2	17	theme	antibodies	469:478	arg1	antibodies					469:478	neutralizing antibodies	456:478	neutralizing antibodies	456:478	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 (ACE2) binding and thus is the target of a variety of neutralizing antibodies.
34013268	3	18	theme	molecular	660:668	arg1	dynamics					670:677	all-atom molecular dynamics	651:677	all-atom molecular dynamics simulations	651:689	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work, and performed all-atom molecular dynamics simulations to get insight into the structural dynamics and interactions between S protein and antibodies.
34013268	5	19	theme	conformations	955:967	arg1	Comparison					930:939	Comparison	930:939	Comparison of the glycan conformations between S-only and S-antibody systems	930:1005	Comparison of the glycan conformations between S-only and S-antibody systems reveals the roles of glycans in S-antibody binding.
34013268	7	20	theme	antibody-based	1468:1481	arg1	therapy					1483:1489	antibody-based therapy	1468:1489	antibody-based therapy	1468:1489	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.
34013268	2	21	theme	human	364:368	arg1	enzyme					393:398	human angiotensin-converting enzyme 2	364:400	human angiotensin-converting enzyme 2 (ACE2) binding	364:415	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 (ACE2) binding and thus is the target of a variety of neutralizing antibodies.
34013268	2	21	theme	human	364:368	arg1	ACE2					403:406	ACE2	403:406	ACE2	403:406	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 (ACE2) binding and thus is the target of a variety of neutralizing antibodies.
34013268	3	22	theme	dynamics	670:677	arg1	simulations					679:689	all-atom molecular dynamics simulations	651:689	all-atom molecular dynamics simulations	651:689	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work, and performed all-atom molecular dynamics simulations to get insight into the structural dynamics and interactions between S protein and antibodies.
34013268	2	23	theme	neutralizing	456:467	arg1	antibodies					469:478	neutralizing antibodies	456:478	neutralizing antibodies	456:478	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 (ACE2) binding and thus is the target of a variety of neutralizing antibodies.
34013268	3	24	theme	trimer-antibody	514:528	arg1	structures					538:547	various S trimer-antibody complex structures	504:547	various S trimer-antibody complex structures	504:547	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work, and performed all-atom molecular dynamics simulations to get insight into the structural dynamics and interactions between S protein and antibodies.
34013268	7	25	theme	better	1228:1233	arg1	understanding					1235:1247	a better understanding	1226:1247	a better understanding of S-antibody interactions	1226:1274	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.
34013268	1	26	theme	public	179:184	arg1	crisis					193:198	a public health crisis	177:198	a public health crisis	177:198	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.
34013268	0	27	theme	Dynamic	0:6	arg1	Interactions					8:19	Dynamic Interactions	0:19	Dynamic Interactions of Fully Glycosylated SARS-CoV-2 Spike Protein with Various Antibodies.	0:91	Dynamic Interactions of Fully Glycosylated SARS-CoV-2 Spike Protein with Various Antibodies.
34013268	4	28	theme	critical	816:823	arg1	residues					807:814	the residues	803:814	the residues critical for S-antibody binding	803:846	Investigation of the residues critical for S-antibody binding allows us to predict the potential influence of mutations in SARS-CoV-2 variants.
34013268	2	29	dep	spike	315:319	arg1	S					322:322	S	322:322	S	322:322	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 (ACE2) binding and thus is the target of a variety of neutralizing antibodies.
34013268	7	30	theme	therapy	1483:1489	arg1	development					1441:1451	the development	1437:1451	the development of vaccine and antibody-based therapy	1437:1489	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.
34013268	1	31	theme	health	186:191	arg1	crisis					193:198	a public health crisis	177:198	a public health crisis	177:198	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.
34013268	3	32	theme	various	504:510	arg1	structures					538:547	various S trimer-antibody complex structures	504:547	various S trimer-antibody complex structures	504:547	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work, and performed all-atom molecular dynamics simulations to get insight into the structural dynamics and interactions between S protein and antibodies.
34013268	5	33	from	roles	1019:1023	arg1	binding					1050:1056	S-antibody binding	1039:1056	S-antibody binding	1039:1056	Comparison of the glycan conformations between S-only and S-antibody systems reveals the roles of glycans in S-antibody binding.
34013268	7	34	from	influences	1359:1368	arg1	interactions					1398:1409	S-antibody interactions	1387:1409	S-antibody interactions	1387:1409	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.
34013268	3	35	theme	complex	530:536	arg1	structures					538:547	various S trimer-antibody complex structures	504:547	various S trimer-antibody complex structures	504:547	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work, and performed all-atom molecular dynamics simulations to get insight into the structural dynamics and interactions between S protein and antibodies.
34013268	7	36	theme	S-antibody	1387:1396	arg1	interactions					1398:1409	S-antibody interactions	1387:1409	S-antibody interactions	1387:1409	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.
34013268	3	37	theme	models	598:603	arg1	basis					556:560	the basis	552:560	the basis of the fully glycosylated S protein models described in our previous work	552:634	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work, and performed all-atom molecular dynamics simulations to get insight into the structural dynamics and interactions between S protein and antibodies.
34013268	1	38	theme	severe	107:112	arg1	coronavirus					141:151	severe acute respiratory syndrome coronavirus 2	107:153	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	107:166	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.
34013268	1	38	theme	severe	107:112	arg1	SARS-CoV-2					156:165	SARS-CoV-2	156:165	SARS-CoV-2	156:165	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.
34013268	7	39	theme	mutation	1375:1382	arg1	influences					1359:1368	the influences	1355:1368	the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy	1355:1489	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.
34013268	0	40	theme	SARS-CoV-2	43:52	arg1	Protein					60:66	Fully Glycosylated SARS-CoV-2 Spike Protein	24:66	Fully Glycosylated SARS-CoV-2 Spike Protein	24:66	Dynamic Interactions of Fully Glycosylated SARS-CoV-2 Spike Protein with Various Antibodies.
34013268	7	41	theme	interactions	1263:1274	arg1	understanding					1235:1247	a better understanding	1226:1247	a better understanding of S-antibody interactions	1226:1274	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.
34013268	6	42	from	influences	1120:1129	arg1	motion					1150:1155	the motion	1146:1155	the motion of S protein receptor binding domains	1146:1193	In addition, we explored the antibody binding modes, and the influences of antibody on the motion of S protein receptor binding domains.
34013268	7	43	theme	S-antibody	1252:1261	arg1	interactions					1263:1274	S-antibody interactions	1252:1274	S-antibody interactions	1252:1274	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.
34013268	0	44	theme	Glycosylated	30:41	arg1	Protein					60:66	Fully Glycosylated SARS-CoV-2 Spike Protein	24:66	Fully Glycosylated SARS-CoV-2 Spike Protein	24:66	Dynamic Interactions of Fully Glycosylated SARS-CoV-2 Spike Protein with Various Antibodies.
34013268	4	45	theme	mutations	896:904	arg1	influence					883:891	the potential influence	869:891	the potential influence of mutations in SARS-CoV-2 variants	869:927	Investigation of the residues critical for S-antibody binding allows us to predict the potential influence of mutations in SARS-CoV-2 variants.
34013268	3	46	theme	S	588:588	arg1	models					598:603	the fully glycosylated S protein models	565:603	the fully glycosylated S protein models described in our previous work	565:634	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work, and performed all-atom molecular dynamics simulations to get insight into the structural dynamics and interactions between S protein and antibodies.
34013268	2	47	theme	enzyme	393:398	arg1	binding					409:415	human angiotensin-converting enzyme 2 (ACE2) binding	364:415	human angiotensin-converting enzyme 2 (ACE2) binding	364:415	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 (ACE2) binding and thus is the target of a variety of neutralizing antibodies.
34013268	0	48	theme	Protein	60:66	arg1	Interactions					8:19	Dynamic Interactions	0:19	Dynamic Interactions of Fully Glycosylated SARS-CoV-2 Spike Protein with Various Antibodies.	0:91	Dynamic Interactions of Fully Glycosylated SARS-CoV-2 Spike Protein with Various Antibodies.
34013268	3	49	theme	protein	590:596	arg1	models					598:603	the fully glycosylated S protein models	565:603	the fully glycosylated S protein models described in our previous work	565:634	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work, and performed all-atom molecular dynamics simulations to get insight into the structural dynamics and interactions between S protein and antibodies.
34013268	6	50	theme	domains	1187:1193	arg1	motion					1150:1155	the motion	1146:1155	the motion of S protein receptor binding domains	1146:1193	In addition, we explored the antibody binding modes, and the influences of antibody on the motion of S protein receptor binding domains.
34013268	1	51	theme	acute	114:118	arg1	coronavirus					141:151	severe acute respiratory syndrome coronavirus 2	107:153	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	107:166	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.
34013268	1	51	theme	acute	114:118	arg1	SARS-CoV-2					156:165	SARS-CoV-2	156:165	SARS-CoV-2	156:165	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.
34013268	0	52	theme	Spike	54:58	arg1	Protein					60:66	Fully Glycosylated SARS-CoV-2 Spike Protein	24:66	Fully Glycosylated SARS-CoV-2 Spike Protein	24:66	Dynamic Interactions of Fully Glycosylated SARS-CoV-2 Spike Protein with Various Antibodies.
34013268	3	53	theme	structural	715:724	arg1	dynamics					726:733	the structural dynamics	711:733	the structural dynamics	711:733	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work, and performed all-atom molecular dynamics simulations to get insight into the structural dynamics and interactions between S protein and antibodies.
34013268	7	54	theme	simulation-based	1285:1300	arg1	maps					1325:1328	the simulation-based S-antibody interaction maps	1281:1328	the simulation-based S-antibody interaction maps	1281:1328	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.
34013268	3	55	theme	previous	622:629	arg1	work					631:634	our previous work	618:634	our previous work	618:634	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work, and performed all-atom molecular dynamics simulations to get insight into the structural dynamics and interactions between S protein and antibodies.
34013268	6	56	theme	binding	1179:1185	arg1	domains					1187:1193	S protein receptor binding domains	1160:1193	S protein receptor binding domains	1160:1193	In addition, we explored the antibody binding modes, and the influences of antibody on the motion of S protein receptor binding domains.
34013268	0	57	with	Interactions	8:19	arg1	Antibodies					81:90	Various Antibodies	73:90	Various Antibodies	73:90	Dynamic Interactions of Fully Glycosylated SARS-CoV-2 Spike Protein with Various Antibodies.
34013268	1	58	theme	respiratory	120:130	arg1	coronavirus					141:151	severe acute respiratory syndrome coronavirus 2	107:153	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	107:166	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.
34013268	1	58	theme	respiratory	120:130	arg1	SARS-CoV-2					156:165	SARS-CoV-2	156:165	SARS-CoV-2	156:165	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.
34013268	3	59	gly	glycosylated	575:586	arg1	models					598:603	the fully glycosylated S protein models	565:603	the fully glycosylated S protein models described in our previous work	565:634	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work, and performed all-atom molecular dynamics simulations to get insight into the structural dynamics and interactions between S protein and antibodies.
34013268	4	60	theme	potential	873:881	arg1	influence					883:891	the potential influence	869:891	the potential influence of mutations in SARS-CoV-2 variants	869:927	Investigation of the residues critical for S-antibody binding allows us to predict the potential influence of mutations in SARS-CoV-2 variants.
34013268	5	61	theme	glycans	1028:1034	arg1	roles					1019:1023	the roles	1015:1023	the roles of glycans in S-antibody binding	1015:1056	Comparison of the glycan conformations between S-only and S-antibody systems reveals the roles of glycans in S-antibody binding.
34013268	6	62	theme	receptor	1170:1177	arg1	domains					1187:1193	S protein receptor binding domains	1160:1193	S protein receptor binding domains	1160:1193	In addition, we explored the antibody binding modes, and the influences of antibody on the motion of S protein receptor binding domains.
34013268	6	63	from	modes	1105:1109	arg1	motion					1150:1155	the motion	1146:1155	the motion of S protein receptor binding domains	1146:1193	In addition, we explored the antibody binding modes, and the influences of antibody on the motion of S protein receptor binding domains.
34013268	1	64	theme	syndrome	132:139	arg1	coronavirus					141:151	severe acute respiratory syndrome coronavirus 2	107:153	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	107:166	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.
34013268	1	64	theme	syndrome	132:139	arg1	SARS-CoV-2					156:165	SARS-CoV-2	156:165	SARS-CoV-2	156:165	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.
34013268	6	65	theme	antibody	1088:1095	arg1	modes					1105:1109	the antibody binding modes	1084:1109	the antibody binding modes	1084:1109	In addition, we explored the antibody binding modes, and the influences of antibody on the motion of S protein receptor binding domains.
34013268	5	66	dep	S-only	977:982	arg1	systems					999:1005	systems	999:1005	systems	999:1005	Comparison of the glycan conformations between S-only and S-antibody systems reveals the roles of glycans in S-antibody binding.
34013268	6	67	theme	antibody	1134:1141	arg1	influences					1120:1129	the influences	1116:1129	the influences of antibody on the motion of S protein receptor binding domains	1116:1193	In addition, we explored the antibody binding modes, and the influences of antibody on the motion of S protein receptor binding domains.
34013268	6	67	theme	antibody	1134:1141	arg1	modes					1105:1109	the antibody binding modes	1084:1109	the antibody binding modes	1084:1109	In addition, we explored the antibody binding modes, and the influences of antibody on the motion of S protein receptor binding domains.
34013268	3	68	theme	glycosylated	575:586	arg1	models					598:603	the fully glycosylated S protein models	565:603	the fully glycosylated S protein models described in our previous work	565:634	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work, and performed all-atom molecular dynamics simulations to get insight into the structural dynamics and interactions between S protein and antibodies.
34013268	1	69	theme	coronavirus	141:151	arg1	spread					97:102	The spread	93:102	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	93:166	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.
34013268	6	70	theme	binding	1097:1103	arg1	modes					1105:1109	the antibody binding modes	1084:1109	the antibody binding modes	1084:1109	In addition, we explored the antibody binding modes, and the influences of antibody on the motion of S protein receptor binding domains.
34013268	0	71	theme	Various	73:79	arg1	Antibodies					81:90	Various Antibodies	73:90	Various Antibodies	73:90	Dynamic Interactions of Fully Glycosylated SARS-CoV-2 Spike Protein with Various Antibodies.
34013268	5	72	theme	S-antibody	1039:1048	arg1	binding					1050:1056	S-antibody binding	1039:1056	S-antibody binding	1039:1056	Comparison of the glycan conformations between S-only and S-antibody systems reveals the roles of glycans in S-antibody binding.
34013268	6	73	theme	S	1160:1160	arg1	domains					1187:1193	S protein receptor binding domains	1160:1193	S protein receptor binding domains	1160:1193	In addition, we explored the antibody binding modes, and the influences of antibody on the motion of S protein receptor binding domains.
34013268	2	74	theme	viral	340:344	arg1	envelope					346:353	the viral envelope	336:353	the viral envelope	336:353	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 (ACE2) binding and thus is the target of a variety of neutralizing antibodies.
34013268	7	75	theme	interaction	1313:1323	arg1	maps					1325:1328	the simulation-based S-antibody interaction maps	1281:1328	the simulation-based S-antibody interaction maps	1281:1328	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.
34013268	7	76	theme	S	1373:1373	arg1	mutation					1375:1382	S mutation	1373:1382	S mutation	1373:1382	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.
34013268	3	77	theme	S	760:760	arg1	protein					762:768	S protein	760:768	S protein	760:768	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work, and performed all-atom molecular dynamics simulations to get insight into the structural dynamics and interactions between S protein and antibodies.
32763405	9	0	theme	comparative	1525:1535	arg1	study					1537:1541	This comparative study	1520:1541	This comparative study	1520:1541	This comparative study provided important knowledge of selecting CNF allomorph for fabrication of high-performance CNF/PVA composites.
32763405	8	1	theme	CNFs	1514:1517	arg1	addition					1502:1509	addition	1502:1509	addition of CNFs	1502:1517	All composite films remained excellent transparency with addition of CNFs.
32763405	6	2	theme	PVA	1190:1192	arg1	nanocomposite					1194:1206	PVA nanocomposite	1190:1206	PVA nanocomposite	1190:1206	Addition of CNFs raised onset degradation temperature (Tonset) and thermal decomposition temperature (Tmax) of PVA nanocomposite, while decreased the melting temperature (Tm).
32763405	1	3	theme	sulfuric	391:398	arg1	hydrolysis					405:414	sulfuric acid hydrolysis	391:414	sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization	391:530	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	3	theme	sulfuric	391:398	arg1	treatments					365:374	mechanical treatments	354:374	mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II)	354:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	4	4	theme	CNF	847:849	arg1	allomorph					851:859	CNF allomorph	847:859	CNF allomorph	847:859	CNF allomorph had significant impact on crystal structure, mechanical and thermal properties of the PVA composites.
32763405	7	5	theme	10 wt	1331:1335	arg1	loading					1342:1348	10 wt% CNF loading	1331:1348	10 wt% CNF loading	1331:1348	Temperature window (Tonset - Tm) for the melt processing of nanocomposites (10 wt% CNF loading) were increased 2.8, 3.2 and 2.5 times for CNF-I/PVA, RNF-II/PVA and MNF-II/PVA, respectively.
32763405	7	5	theme	10 wt	1331:1335	arg1	nanocomposites					1315:1328	nanocomposites	1315:1328	nanocomposites (10 wt% CNF loading)	1315:1349	Temperature window (Tonset - Tm) for the melt processing of nanocomposites (10 wt% CNF loading) were increased 2.8, 3.2 and 2.5 times for CNF-I/PVA, RNF-II/PVA and MNF-II/PVA, respectively.
32763405	1	6	from	recrystallization	607:623	arg1	water					628:632	water	628:632	water (RNF-II)	628:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	6	from	recrystallization	607:623	arg1	RNF-II					635:640	RNF-II	635:640	RNF-II	635:640	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	7	7	theme	nanocomposites	1315:1328	arg1	processing					1301:1310	the melt processing	1292:1310	the melt processing of nanocomposites (10 wt% CNF loading)	1292:1349	Temperature window (Tonset - Tm) for the melt processing of nanocomposites (10 wt% CNF loading) were increased 2.8, 3.2 and 2.5 times for CNF-I/PVA, RNF-II/PVA and MNF-II/PVA, respectively.
32763405	2	8	theme	thermal	697:703	arg1	properties					705:714	thermal properties	697:714	thermal properties	697:714	Crystal structure, surface chemistry, morphology and thermal properties of three CNFs were investigated and compared.
32763405	1	9	theme	Cellulose	167:175	arg1	nanofibrils					177:187	Cellulose nanofibrils	167:187	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs	167:229	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	9	theme	Cellulose	167:175	arg1	CNFs					190:193	CNFs	190:193	CNFs	190:193	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	6	10	theme	decomposition	1154:1166	arg1	Tmax					1181:1184	Tmax	1181:1184	Tmax	1181:1184	Addition of CNFs raised onset degradation temperature (Tonset) and thermal decomposition temperature (Tmax) of PVA nanocomposite, while decreased the melting temperature (Tm).
32763405	6	10	theme	decomposition	1154:1166	arg1	temperature					1168:1178	thermal decomposition temperature	1146:1178	thermal decomposition temperature (Tmax)	1146:1185	Addition of CNFs raised onset degradation temperature (Tonset) and thermal decomposition temperature (Tmax) of PVA nanocomposite, while decreased the melting temperature (Tm).
32763405	2	11	theme	CNFs	725:728	arg1	morphology					682:691	morphology	682:691	morphology	682:691	Crystal structure, surface chemistry, morphology and thermal properties of three CNFs were investigated and compared.
32763405	2	11	theme	CNFs	725:728	arg1	structure					652:660	Crystal structure	644:660	Crystal structure	644:660	Crystal structure, surface chemistry, morphology and thermal properties of three CNFs were investigated and compared.
32763405	2	11	theme	CNFs	725:728	arg1	chemistry					671:679	surface chemistry	663:679	surface chemistry	663:679	Crystal structure, surface chemistry, morphology and thermal properties of three CNFs were investigated and compared.
32763405	2	11	theme	CNFs	725:728	arg1	properties					705:714	thermal properties	697:714	thermal properties	697:714	Crystal structure, surface chemistry, morphology and thermal properties of three CNFs were investigated and compared.
32763405	6	12	theme	thermal	1146:1152	arg1	Tmax					1181:1184	Tmax	1181:1184	Tmax	1181:1184	Addition of CNFs raised onset degradation temperature (Tonset) and thermal decomposition temperature (Tmax) of PVA nanocomposite, while decreased the melting temperature (Tm).
32763405	6	12	theme	thermal	1146:1152	arg1	temperature					1168:1178	thermal decomposition temperature	1146:1178	thermal decomposition temperature (Tmax)	1146:1185	Addition of CNFs raised onset degradation temperature (Tonset) and thermal decomposition temperature (Tmax) of PVA nanocomposite, while decreased the melting temperature (Tm).
32763405	0	13	theme	improved	112:119	arg1	stability					129:137	improved thermal stability	112:137	improved thermal stability	112:137	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films with high mechanical properties, improved thermal stability and excellent transparency.
32763405	1	14	theme	classical	381:389	arg1	hydrolysis					405:414	sulfuric acid hydrolysis	391:414	sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization	391:530	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	14	theme	classical	381:389	arg1	treatments					365:374	mechanical treatments	354:374	mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II)	354:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	9	15	theme	high-performance	1618:1633	arg1	composites					1643:1652	high-performance CNF/PVA composites	1618:1652	high-performance CNF/PVA composites	1618:1652	This comparative study provided important knowledge of selecting CNF allomorph for fabrication of high-performance CNF/PVA composites.
32763405	2	16	theme	Crystal	644:650	arg1	structure					652:660	Crystal structure	644:660	Crystal structure	644:660	Crystal structure, surface chemistry, morphology and thermal properties of three CNFs were investigated and compared.
32763405	1	17	theme	acid	400:403	arg1	hydrolysis					405:414	sulfuric acid hydrolysis	391:414	sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization	391:530	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	17	theme	acid	400:403	arg1	treatments					365:374	mechanical treatments	354:374	mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II)	354:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	9	18	theme	composites	1643:1652	arg1	fabrication					1603:1613	fabrication	1603:1613	fabrication of high-performance CNF/PVA composites	1603:1652	This comparative study provided important knowledge of selecting CNF allomorph for fabrication of high-performance CNF/PVA composites.
32763405	7	19	theme	Temperature	1255:1265	arg1	Tonset - Tm					1275:1285	Tonset - Tm	1275:1285	Tonset - Tm	1275:1285	Temperature window (Tonset - Tm) for the melt processing of nanocomposites (10 wt% CNF loading) were increased 2.8, 3.2 and 2.5 times for CNF-I/PVA, RNF-II/PVA and MNF-II/PVA, respectively.
32763405	7	19	theme	Temperature	1255:1265	arg1	window					1267:1272	Temperature window	1255:1272	Temperature window (Tonset - Tm) for the melt processing of nanocomposites (10 wt% CNF loading)	1255:1349	Temperature window (Tonset - Tm) for the melt processing of nanocomposites (10 wt% CNF loading) were increased 2.8, 3.2 and 2.5 times for CNF-I/PVA, RNF-II/PVA and MNF-II/PVA, respectively.
32763405	0	20	theme	excellent	143:151	arg1	transparency					153:164	excellent transparency	143:164	excellent transparency	143:164	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films with high mechanical properties, improved thermal stability and excellent transparency.
32763405	3	21	theme	%	783:783	arg1	CNFs					768:771	Three CNFs	762:771	Three CNFs of 2-10 wt%	762:783	Three CNFs of 2-10 wt% were mixed with PVA to prepare biodegradable composite films.
32763405	6	22	theme	degradation	1109:1119	arg1	temperature					1121:1131	onset degradation temperature	1103:1131	onset degradation temperature (Tonset)	1103:1140	Addition of CNFs raised onset degradation temperature (Tonset) and thermal decomposition temperature (Tmax) of PVA nanocomposite, while decreased the melting temperature (Tm).
32763405	6	22	theme	degradation	1109:1119	arg1	Tonset					1134:1139	Tonset	1134:1139	Tonset	1134:1139	Addition of CNFs raised onset degradation temperature (Tonset) and thermal decomposition temperature (Tmax) of PVA nanocomposite, while decreased the melting temperature (Tm).
32763405	1	23	theme	cellulose	550:558	arg1	hydrolysis					405:414	sulfuric acid hydrolysis	391:414	sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization	391:530	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	23	theme	cellulose	550:558	arg1	hydrolysis					536:545	hydrolysis	536:545	hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II)	536:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	23	theme	cellulose	550:558	arg1	treatments					365:374	mechanical treatments	354:374	mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II)	354:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	0	24	theme	vinyl	48:52	arg1	films					73:77	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films	0:77	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films with high mechanical properties, improved thermal stability and excellent transparency.	0:165	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films with high mechanical properties, improved thermal stability and excellent transparency.
32763405	1	25	theme	different	321:329	arg1	conditions					331:340	three different conditions	315:340	three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II)	315:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	9	26	theme	important	1552:1560	arg1	knowledge					1562:1570	important knowledge	1552:1570	important knowledge of selecting CNF allomorph for fabrication of high-performance CNF/PVA composites	1552:1652	This comparative study provided important knowledge of selecting CNF allomorph for fabrication of high-performance CNF/PVA composites.
32763405	4	27	theme	crystal	887:893	arg1	structure					895:903	crystal structure	887:903	crystal structure	887:903	CNF allomorph had significant impact on crystal structure, mechanical and thermal properties of the PVA composites.
32763405	4	27	theme	crystal	887:893	arg1	properties					929:938	mechanical and thermal properties	906:938	mechanical and thermal properties of the PVA composites	906:960	CNF allomorph had significant impact on crystal structure, mechanical and thermal properties of the PVA composites.
32763405	1	28	theme	sulfuric	578:585	arg1	acid					587:590	concentrated sulfuric acid	565:590	concentrated sulfuric acid	565:590	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	29	theme	mechanical	354:363	arg1	treatments					365:374	mechanical treatments	354:374	mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II)	354:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	29	theme	mechanical	354:363	arg1	hydrolysis					536:545	hydrolysis	536:545	hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II)	536:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	29	theme	mechanical	354:363	arg1	hydrolysis					405:414	sulfuric acid hydrolysis	391:414	sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization	391:530	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	7	30	theme	%	1336:1336	arg1	loading					1342:1348	10 wt% CNF loading	1331:1348	10 wt% CNF loading	1331:1348	Temperature window (Tonset - Tm) for the melt processing of nanocomposites (10 wt% CNF loading) were increased 2.8, 3.2 and 2.5 times for CNF-I/PVA, RNF-II/PVA and MNF-II/PVA, respectively.
32763405	7	30	theme	%	1336:1336	arg1	nanocomposites					1315:1328	nanocomposites	1315:1328	nanocomposites (10 wt% CNF loading)	1315:1349	Temperature window (Tonset - Tm) for the melt processing of nanocomposites (10 wt% CNF loading) were increased 2.8, 3.2 and 2.5 times for CNF-I/PVA, RNF-II/PVA and MNF-II/PVA, respectively.
32763405	1	31	dep	hydrolysis	459:468	arg1	ii					441:442	ii	441:442	ii	441:442	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	0	32	theme	high	84:87	arg1	properties					100:109	high mechanical properties	84:109	high mechanical properties	84:109	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films with high mechanical properties, improved thermal stability and excellent transparency.
32763405	3	33	theme	composite	830:838	arg1	films					840:844	biodegradable composite films	816:844	biodegradable composite films	816:844	Three CNFs of 2-10 wt% were mixed with PVA to prepare biodegradable composite films.
32763405	0	34	theme	Biodegradable	0:12	arg1	films					73:77	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films	0:77	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films with high mechanical properties, improved thermal stability and excellent transparency.	0:165	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films with high mechanical properties, improved thermal stability and excellent transparency.
32763405	1	35	theme	sulfuric	260:267	arg1	hydrolysis					274:283	sulfuric acid hydrolysis	260:283	sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II)	260:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	36	theme	cellulose	419:427	arg1	hydrolysis					405:414	sulfuric acid hydrolysis	391:414	sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization	391:530	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	36	theme	cellulose	419:427	arg1	hydrolysis					536:545	hydrolysis	536:545	hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II)	536:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	36	theme	cellulose	419:427	arg1	treatments					365:374	mechanical treatments	354:374	mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II)	354:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	37	theme	solubilization	517:530	arg1	hydrolysis					405:414	sulfuric acid hydrolysis	391:414	sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization	391:530	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	37	theme	solubilization	517:530	arg1	hydrolysis					536:545	hydrolysis	536:545	hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II)	536:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	37	theme	solubilization	517:530	arg1	treatments					365:374	mechanical treatments	354:374	mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II)	354:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	38	theme	eucalyptus	288:297	arg1	cellulose					299:307	eucalyptus cellulose	288:307	eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II)	288:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	4	39	theme	composites	951:960	arg1	structure					895:903	crystal structure	887:903	crystal structure	887:903	CNF allomorph had significant impact on crystal structure, mechanical and thermal properties of the PVA composites.
32763405	4	39	theme	composites	951:960	arg1	properties					929:938	mechanical and thermal properties	906:938	mechanical and thermal properties of the PVA composites	906:960	CNF allomorph had significant impact on crystal structure, mechanical and thermal properties of the PVA composites.
32763405	7	40	theme	CNF	1338:1340	arg1	loading					1342:1348	10 wt% CNF loading	1331:1348	10 wt% CNF loading	1331:1348	Temperature window (Tonset - Tm) for the melt processing of nanocomposites (10 wt% CNF loading) were increased 2.8, 3.2 and 2.5 times for CNF-I/PVA, RNF-II/PVA and MNF-II/PVA, respectively.
32763405	7	40	theme	CNF	1338:1340	arg1	nanocomposites					1315:1328	nanocomposites	1315:1328	nanocomposites (10 wt% CNF loading)	1315:1349	Temperature window (Tonset - Tm) for the melt processing of nanocomposites (10 wt% CNF loading) were increased 2.8, 3.2 and 2.5 times for CNF-I/PVA, RNF-II/PVA and MNF-II/PVA, respectively.
32763405	0	41	theme	nanofibrils/poly	31:46	arg1	films					73:77	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films	0:77	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films with high mechanical properties, improved thermal stability and excellent transparency.	0:165	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films with high mechanical properties, improved thermal stability and excellent transparency.
32763405	5	42	theme	Young	975:979	arg1	modulus					983:989	The maximum Young's modulus	963:989	The maximum Young's modulus of CNF-I/PVA, RNF-II/PVA and MNF-II/PVA	963:1029	The maximum Young's modulus of CNF-I/PVA, RNF-II/PVA and MNF-II/PVA were increased by 62, 32 and 44%, respectively.
32763405	1	43	dep	treatments	365:374	arg1	hydrolysis					405:414	sulfuric acid hydrolysis	391:414	sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization	391:530	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	43	dep	treatments	365:374	arg1	hydrolysis					536:545	hydrolysis	536:545	hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II)	536:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	43	dep	treatments	365:374	arg1	treatments					365:374	mechanical treatments	354:374	mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II)	354:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	8	44	theme	excellent	1474:1482	arg1	transparency					1484:1495	excellent transparency	1474:1495	excellent transparency	1474:1495	All composite films remained excellent transparency with addition of CNFs.
32763405	1	45	theme	cellulose	484:492	arg1	hydrolysis					459:468	(ii) sulfuric acid hydrolysis	440:468	(ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II)	440:504	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	45	theme	cellulose	484:492	arg1	CNF-I					432:436	CNF-I	432:436	CNF-I	432:436	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	45	theme	cellulose	484:492	arg1	solubilization					517:530	(iii) solubilization	511:530	(iii) solubilization	511:530	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	45	theme	cellulose	484:492	arg1	cellulose					419:427	cellulose I	419:429	cellulose I (CNF-I)	419:437	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	6	46	theme	nanocomposite	1194:1206	arg1	temperature					1121:1131	onset degradation temperature	1103:1131	onset degradation temperature (Tonset)	1103:1140	Addition of CNFs raised onset degradation temperature (Tonset) and thermal decomposition temperature (Tmax) of PVA nanocomposite, while decreased the melting temperature (Tm).
32763405	6	46	theme	nanocomposite	1194:1206	arg1	Tmax					1181:1184	Tmax	1181:1184	Tmax	1181:1184	Addition of CNFs raised onset degradation temperature (Tonset) and thermal decomposition temperature (Tmax) of PVA nanocomposite, while decreased the melting temperature (Tm).
32763405	6	46	theme	nanocomposite	1194:1206	arg1	temperature					1168:1178	thermal decomposition temperature	1146:1178	thermal decomposition temperature (Tmax)	1146:1185	Addition of CNFs raised onset degradation temperature (Tonset) and thermal decomposition temperature (Tmax) of PVA nanocomposite, while decreased the melting temperature (Tm).
32763405	6	46	theme	nanocomposite	1194:1206	arg1	Tonset					1134:1139	Tonset	1134:1139	Tonset	1134:1139	Addition of CNFs raised onset degradation temperature (Tonset) and thermal decomposition temperature (Tmax) of PVA nanocomposite, while decreased the melting temperature (Tm).
32763405	7	47	theme	increased	1356:1364	arg1	times					1383:1387	2.8, 3.2 and 2.5 times	1366:1387	increased 2.8, 3.2 and 2.5 times	1356:1387	Temperature window (Tonset - Tm) for the melt processing of nanocomposites (10 wt% CNF loading) were increased 2.8, 3.2 and 2.5 times for CNF-I/PVA, RNF-II/PVA and MNF-II/PVA, respectively.
32763405	1	48	theme	acid	454:457	arg1	hydrolysis					459:468	(ii) sulfuric acid hydrolysis	440:468	(ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II)	440:504	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	6	49	theme	CNFs	1091:1094	arg1	Addition					1079:1086	Addition	1079:1086	Addition of CNFs	1079:1094	Addition of CNFs raised onset degradation temperature (Tonset) and thermal decomposition temperature (Tmax) of PVA nanocomposite, while decreased the melting temperature (Tm).
32763405	1	50	dep	hydrolysis	405:414	arg1	i					378:378	i	378:378	i	378:378	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	51	from	acid	587:590	arg1	water					628:632	water	628:632	water (RNF-II)	628:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	51	from	acid	587:590	arg1	RNF-II					635:640	RNF-II	635:640	RNF-II	635:640	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	9	52	theme	CNF	1585:1587	arg1	allomorph					1589:1597	CNF allomorph	1585:1597	CNF allomorph	1585:1597	This comparative study provided important knowledge of selecting CNF allomorph for fabrication of high-performance CNF/PVA composites.
32763405	1	53	theme	subsequent	596:605	arg1	recrystallization					607:623	subsequent recrystallization	596:623	subsequent recrystallization in water (RNF-II)	596:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	0	54	with	films	73:77	arg1	properties					100:109	high mechanical properties	84:109	high mechanical properties	84:109	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films with high mechanical properties, improved thermal stability and excellent transparency.
32763405	0	54	with	films	73:77	arg1	stability					129:137	improved thermal stability	112:137	improved thermal stability	112:137	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films with high mechanical properties, improved thermal stability and excellent transparency.
32763405	0	54	with	films	73:77	arg1	transparency					153:164	excellent transparency	143:164	excellent transparency	143:164	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films with high mechanical properties, improved thermal stability and excellent transparency.
32763405	7	55	theme	melt	1296:1299	arg1	processing					1301:1310	the melt processing	1292:1310	the melt processing of nanocomposites (10 wt% CNF loading)	1292:1349	Temperature window (Tonset - Tm) for the melt processing of nanocomposites (10 wt% CNF loading) were increased 2.8, 3.2 and 2.5 times for CNF-I/PVA, RNF-II/PVA and MNF-II/PVA, respectively.
32763405	0	56	theme	thermal	121:127	arg1	stability					129:137	improved thermal stability	112:137	improved thermal stability	112:137	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films with high mechanical properties, improved thermal stability and excellent transparency.
32763405	5	57	theme	RNF-II/PVA	1005:1014	arg1	modulus					983:989	The maximum Young's modulus	963:989	The maximum Young's modulus of CNF-I/PVA, RNF-II/PVA and MNF-II/PVA	963:1029	The maximum Young's modulus of CNF-I/PVA, RNF-II/PVA and MNF-II/PVA were increased by 62, 32 and 44%, respectively.
32763405	4	58	theme	significant	865:875	arg1	impact					877:882	significant impact	865:882	significant impact	865:882	CNF allomorph had significant impact on crystal structure, mechanical and thermal properties of the PVA composites.
32763405	5	59	theme	MNF-II/PVA	1020:1029	arg1	modulus					983:989	The maximum Young's modulus	963:989	The maximum Young's modulus of CNF-I/PVA, RNF-II/PVA and MNF-II/PVA	963:1029	The maximum Young's modulus of CNF-I/PVA, RNF-II/PVA and MNF-II/PVA were increased by 62, 32 and 44%, respectively.
32763405	9	60	theme	CNF/PVA	1635:1641	arg1	composites					1643:1652	high-performance CNF/PVA composites	1618:1652	high-performance CNF/PVA composites	1618:1652	This comparative study provided important knowledge of selecting CNF allomorph for fabrication of high-performance CNF/PVA composites.
32763405	0	61	theme	alcohol	54:60	arg1	films					73:77	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films	0:77	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films with high mechanical properties, improved thermal stability and excellent transparency.	0:165	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films with high mechanical properties, improved thermal stability and excellent transparency.
32763405	5	62	theme	CNF-I/PVA	994:1002	arg1	modulus					983:989	The maximum Young's modulus	963:989	The maximum Young's modulus of CNF-I/PVA, RNF-II/PVA and MNF-II/PVA	963:1029	The maximum Young's modulus of CNF-I/PVA, RNF-II/PVA and MNF-II/PVA were increased by 62, 32 and 44%, respectively.
32763405	4	63	theme	mechanical	906:915	arg1	structure					895:903	crystal structure	887:903	crystal structure	887:903	CNF allomorph had significant impact on crystal structure, mechanical and thermal properties of the PVA composites.
32763405	4	63	theme	mechanical	906:915	arg1	properties					929:938	mechanical and thermal properties	906:938	mechanical and thermal properties of the PVA composites	906:960	CNF allomorph had significant impact on crystal structure, mechanical and thermal properties of the PVA composites.
32763405	6	64	theme	onset	1103:1107	arg1	temperature					1121:1131	onset degradation temperature	1103:1131	onset degradation temperature (Tonset)	1103:1140	Addition of CNFs raised onset degradation temperature (Tonset) and thermal decomposition temperature (Tmax) of PVA nanocomposite, while decreased the melting temperature (Tm).
32763405	6	64	theme	onset	1103:1107	arg1	Tonset					1134:1139	Tonset	1134:1139	Tonset	1134:1139	Addition of CNFs raised onset degradation temperature (Tonset) and thermal decomposition temperature (Tmax) of PVA nanocomposite, while decreased the melting temperature (Tm).
32763405	2	65	theme	surface	663:669	arg1	chemistry					671:679	surface chemistry	663:679	surface chemistry	663:679	Crystal structure, surface chemistry, morphology and thermal properties of three CNFs were investigated and compared.
32763405	0	66	theme	composite	63:71	arg1	films					73:77	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films	0:77	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films with high mechanical properties, improved thermal stability and excellent transparency.	0:165	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films with high mechanical properties, improved thermal stability and excellent transparency.
32763405	3	67	theme	biodegradable	816:828	arg1	films					840:844	biodegradable composite films	816:844	biodegradable composite films	816:844	Three CNFs of 2-10 wt% were mixed with PVA to prepare biodegradable composite films.
32763405	4	68	theme	thermal	921:927	arg1	structure					895:903	crystal structure	887:903	crystal structure	887:903	CNF allomorph had significant impact on crystal structure, mechanical and thermal properties of the PVA composites.
32763405	4	68	theme	thermal	921:927	arg1	properties					929:938	mechanical and thermal properties	906:938	mechanical and thermal properties of the PVA composites	906:960	CNF allomorph had significant impact on crystal structure, mechanical and thermal properties of the PVA composites.
32763405	1	69	theme	concentrated	565:576	arg1	acid					587:590	concentrated sulfuric acid	565:590	concentrated sulfuric acid	565:590	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	5	70	theme	44	1060:1061	arg1	%					1062:1062	62, 32 and 44%	1049:1062	%	1062:1062	The maximum Young's modulus of CNF-I/PVA, RNF-II/PVA and MNF-II/PVA were increased by 62, 32 and 44%, respectively.
32763405	0	71	theme	mechanical	89:98	arg1	properties					100:109	high mechanical properties	84:109	high mechanical properties	84:109	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films with high mechanical properties, improved thermal stability and excellent transparency.
32763405	0	72	theme	cellulose	14:22	arg1	films					73:77	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films	0:77	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films with high mechanical properties, improved thermal stability and excellent transparency.	0:165	Biodegradable cellulose I (II) nanofibrils/poly(vinyl alcohol) composite films with high mechanical properties, improved thermal stability and excellent transparency.
32763405	5	73	theme	62	1049:1050	arg1	%					1062:1062	62, 32 and 44%	1049:1062	%	1062:1062	The maximum Young's modulus of CNF-I/PVA, RNF-II/PVA and MNF-II/PVA were increased by 62, 32 and 44%, respectively.
32763405	1	74	theme	acid	269:272	arg1	hydrolysis					274:283	sulfuric acid hydrolysis	260:283	sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II)	260:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	4	75	contain	had	861:863	arg2	impact					877:882	significant impact	865:882	significant impact	865:882	CNF allomorph had significant impact on crystal structure, mechanical and thermal properties of the PVA composites.
32763405	4	75	contain	had	861:863	arg1	allomorph					851:859	CNF allomorph	847:859	CNF allomorph	847:859	CNF allomorph had significant impact on crystal structure, mechanical and thermal properties of the PVA composites.
32763405	8	76	theme	composite	1449:1457	arg1	films					1459:1463	All composite films	1445:1463	All composite films	1445:1463	All composite films remained excellent transparency with addition of CNFs.
32763405	1	77	theme	sulfuric	445:452	arg1	hydrolysis					459:468	(ii) sulfuric acid hydrolysis	440:468	(ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II)	440:504	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	78	theme	cellulose	299:307	arg1	hydrolysis					274:283	sulfuric acid hydrolysis	260:283	sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II)	260:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	79	with	nanofibrils	177:187	arg1	allomorphs					220:229	cellulose I and II allomorphs	201:229	allomorphs	220:229	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	79	with	nanofibrils	177:187	arg1	cellulose					201:209	cellulose I and II allomorphs	201:229	cellulose	201:209	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	5	80	theme	maximum	967:973	arg1	modulus					983:989	The maximum Young's modulus	963:989	The maximum Young's modulus of CNF-I/PVA, RNF-II/PVA and MNF-II/PVA	963:1029	The maximum Young's modulus of CNF-I/PVA, RNF-II/PVA and MNF-II/PVA were increased by 62, 32 and 44%, respectively.
32763405	4	81	theme	PVA	947:949	arg1	composites					951:960	the PVA composites	943:960	the PVA composites	943:960	CNF allomorph had significant impact on crystal structure, mechanical and thermal properties of the PVA composites.
32763405	1	82	theme	hydrolysis	459:468	arg1	hydrolysis					405:414	sulfuric acid hydrolysis	391:414	sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization	391:530	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	82	theme	hydrolysis	459:468	arg1	hydrolysis					536:545	hydrolysis	536:545	hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II)	536:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	82	theme	hydrolysis	459:468	arg1	treatments					365:374	mechanical treatments	354:374	mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II)	354:641	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	6	83	theme	melting	1229:1235	arg1	temperature					1237:1247	the melting temperature	1225:1247	the melting temperature (Tm)	1225:1252	Addition of CNFs raised onset degradation temperature (Tonset) and thermal decomposition temperature (Tmax) of PVA nanocomposite, while decreased the melting temperature (Tm).
32763405	6	83	theme	melting	1229:1235	arg1	Tm					1250:1251	Tm	1250:1251	Tm	1250:1251	Addition of CNFs raised onset degradation temperature (Tonset) and thermal decomposition temperature (Tmax) of PVA nanocomposite, while decreased the melting temperature (Tm).
32763405	1	84	theme	mercerized	473:482	arg1	cellulose					484:492	mercerized cellulose II	473:495	mercerized cellulose II (MNF-II)	473:504	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	84	theme	mercerized	473:482	arg1	MNF-II					498:503	MNF-II	498:503	MNF-II	498:503	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32763405	1	85	dep	solubilization	517:530	arg1	iii					512:514	iii	512:514	iii	512:514	Cellulose nanofibrils (CNFs) with cellulose I and II allomorphs were efficiently prepared by sulfuric acid hydrolysis of eucalyptus cellulose under three different conditions followed by mechanical treatments: (i) classical sulfuric acid hydrolysis of cellulose I (CNF-I), (ii) sulfuric acid hydrolysis of mercerized cellulose II (MNF-II), and (iii) solubilization and hydrolysis of cellulose I by concentrated sulfuric acid and subsequent recrystallization in water (RNF-II).
32781125	6	0	theme	lower	1097:1101	arg1	dose					1115:1118	lower irradiation dose	1097:1118	lower irradiation dose	1097:1118	The thermal stability of the composite increased at lower irradiation dose and higher content of APA.
32781125	2	1	theme	gravimetric	405:415	arg1	analysis					417:424	thermal gravimetric analysis	397:424	thermal gravimetric analysis (TGA)	397:430	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	2	1	theme	gravimetric	405:415	arg1	TGA					427:429	TGA	427:429	TGA	427:429	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	5	2	from	16.61 Å	952:958	arg1	spacing					928:934	an increased interplanar spacing	903:934	an increased interplanar spacing from 13.36 Å to 16.61 Å	903:958	XRD results showed a shift in the MMT diffraction peak from 2θ = 7° to 5.3° with an increased interplanar spacing from 13.36 Å to 16.61 Å, which indicated the interchelated-exfoliated nanostructure of MMT by CS-APA chains.
32781125	12	3	theme	50 mg/l	1926:1932	arg1	concentration					1909:1921	an initial concentration	1898:1921	an initial concentration of 50 mg/l	1898:1932	A dose of 20 mg/50 ml of the composite was sufficient to completely remove MB from a solution with an initial concentration of 50 mg/l.
32781125	10	4	from	3 kGy	1577:1581	arg1	ratios					1538:1543	equal ratios	1532:1543	equal ratios of CS and APA and irradiated at 3 kGy	1532:1581	The composite prepared with equal ratios of CS and APA and irradiated at 3 kGy approached the flocculation efficiency of alum with a remaining turbidity % of 9 and 8% for the composite and alum respectively.
32781125	10	4	from	3 kGy	1577:1581	arg1	APA					1555:1557	APA	1555:1557	APA	1555:1557	The composite prepared with equal ratios of CS and APA and irradiated at 3 kGy approached the flocculation efficiency of alum with a remaining turbidity % of 9 and 8% for the composite and alum respectively.
32781125	10	4	from	3 kGy	1577:1581	arg1	CS					1548:1549	CS	1548:1549	CS	1548:1549	The composite prepared with equal ratios of CS and APA and irradiated at 3 kGy approached the flocculation efficiency of alum with a remaining turbidity % of 9 and 8% for the composite and alum respectively.
32781125	10	5	theme	APA	1555:1557	arg1	ratios					1538:1543	equal ratios	1532:1543	equal ratios of CS and APA and irradiated at 3 kGy	1532:1581	The composite prepared with equal ratios of CS and APA and irradiated at 3 kGy approached the flocculation efficiency of alum with a remaining turbidity % of 9 and 8% for the composite and alum respectively.
32781125	9	6	theme	composites	1431:1440	arg1	performance					1412:1422	The flocculation performance	1395:1422	The flocculation performance of the composites on river water	1395:1455	The flocculation performance of the composites on river water was tested by jar test and compared with alum.
32781125	10	7	theme	CS	1548:1549	arg1	ratios					1538:1543	equal ratios	1532:1543	equal ratios of CS and APA and irradiated at 3 kGy	1532:1581	The composite prepared with equal ratios of CS and APA and irradiated at 3 kGy approached the flocculation efficiency of alum with a remaining turbidity % of 9 and 8% for the composite and alum respectively.
32781125	5	8	theme	MMT	856:858	arg1	peak					872:875	the MMT diffraction peak	852:875	the MMT diffraction peak	852:875	XRD results showed a shift in the MMT diffraction peak from 2θ = 7° to 5.3° with an increased interplanar spacing from 13.36 Å to 16.61 Å, which indicated the interchelated-exfoliated nanostructure of MMT by CS-APA chains.
32781125	3	9	theme	vibrational	583:593	arg1	bands					595:599	vibrational bands	583:599	vibrational bands	583:599	FTIR results revealed the emergence of vibrational bands in the range of 1622 to 1606 cm-1 in the composites spectra indicating the grafting of APA to CS.
32781125	3	10	dep	1606 cm-1	625:633	arg1	to					622:623	to	622:623	to	622:623	FTIR results revealed the emergence of vibrational bands in the range of 1622 to 1606 cm-1 in the composites spectra indicating the grafting of APA to CS.
32781125	5	11	from	shift	843:847	arg1	peak					872:875	the MMT diffraction peak	852:875	the MMT diffraction peak	852:875	XRD results showed a shift in the MMT diffraction peak from 2θ = 7° to 5.3° with an increased interplanar spacing from 13.36 Å to 16.61 Å, which indicated the interchelated-exfoliated nanostructure of MMT by CS-APA chains.
32781125	4	12	dep	composite	811:819	arg1	the					807:809	the	807:809	the	807:809	The shift in the MMT band at 1030 cm-1 to lower wavenumber indicated a strong interchelation interaction in the composite.
32781125	0	13	from	application	84:94	arg1	flocculation					111:122	river water flocculation	99:122	river water flocculation	99:122	Synthesis of montmorillonite/chitosan/ammonium acrylate composite and its potential application in river water flocculation.
32781125	11	14	theme	methylene	1767:1775	arg1	removal					1756:1762	the removal	1752:1762	the removal of methylene blue (MB) from water	1752:1796	Furthermore, the composite was used for the removal of methylene blue (MB) from water.
32781125	1	15	theme	montmorillonite/chitosan-grafted-ammonium	145:185	arg1	polyacrylate					187:198	montmorillonite/chitosan-grafted-ammonium polyacrylate	145:198	montmorillonite/chitosan-grafted-ammonium polyacrylate (MMT/CS-g-APA)	145:213	Composites based on montmorillonite/chitosan-grafted-ammonium polyacrylate (MMT/CS-g-APA) were synthesized by gamma irradiation (3-7 kGy) induced polymerization.
32781125	1	15	theme	montmorillonite/chitosan-grafted-ammonium	145:185	arg1	MMT/CS-g-APA					201:212	MMT/CS-g-APA	201:212	MMT/CS-g-APA	201:212	Composites based on montmorillonite/chitosan-grafted-ammonium polyacrylate (MMT/CS-g-APA) were synthesized by gamma irradiation (3-7 kGy) induced polymerization.
32781125	5	16	dep	16.61 Å	952:958	arg1	to					949:950	to	949:950	to	949:950	XRD results showed a shift in the MMT diffraction peak from 2θ = 7° to 5.3° with an increased interplanar spacing from 13.36 Å to 16.61 Å, which indicated the interchelated-exfoliated nanostructure of MMT by CS-APA chains.
32781125	2	17	theme	Fourier-transform	324:340	arg1	FTIR					365:368	FTIR	365:368	FTIR	365:368	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	2	17	theme	Fourier-transform	324:340	arg1	spectroscopy					351:362	Fourier-transform infrared spectroscopy	324:362	Fourier-transform infrared spectroscopy (FTIR)	324:369	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	5	18	theme	interplanar	916:926	arg1	spacing					928:934	an increased interplanar spacing	903:934	an increased interplanar spacing from 13.36 Å to 16.61 Å	903:958	XRD results showed a shift in the MMT diffraction peak from 2θ = 7° to 5.3° with an increased interplanar spacing from 13.36 Å to 16.61 Å, which indicated the interchelated-exfoliated nanostructure of MMT by CS-APA chains.
32781125	8	19	theme	microsphere	1319:1329	arg1	morphology					1331:1340	the imperfect microsphere morphology	1305:1340	the imperfect microsphere morphology of the composite due to the soft nature of CS-g-APA	1305:1392	SEM showed the imperfect microsphere morphology of the composite due to the soft nature of CS-g-APA.
32781125	3	20	theme	1606 cm-1	625:633	arg1	spectra					653:659	1622 to 1606 cm-1 in the composites spectra	617:659	1622 to 1606 cm-1 in the composites spectra	617:659	FTIR results revealed the emergence of vibrational bands in the range of 1622 to 1606 cm-1 in the composites spectra indicating the grafting of APA to CS.
32781125	2	21	theme	electron	517:524	arg1	microscopy					526:535	transmission electron microscopy	504:535	transmission electron microscopy (TEM)	504:541	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	2	21	theme	electron	517:524	arg1	TEM					538:540	TEM	538:540	TEM	538:540	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	0	22	theme	river	99:103	arg1	flocculation					111:122	river water flocculation	99:122	river water flocculation	99:122	Synthesis of montmorillonite/chitosan/ammonium acrylate composite and its potential application in river water flocculation.
32781125	8	23	theme	composite	1349:1357	arg1	morphology					1331:1340	the imperfect microsphere morphology	1305:1340	the imperfect microsphere morphology of the composite due to the soft nature of CS-g-APA	1305:1392	SEM showed the imperfect microsphere morphology of the composite due to the soft nature of CS-g-APA.
32781125	12	24	with	solution	1884:1891	arg1	concentration					1909:1921	an initial concentration	1898:1921	an initial concentration of 50 mg/l	1898:1932	A dose of 20 mg/50 ml of the composite was sufficient to completely remove MB from a solution with an initial concentration of 50 mg/l.
32781125	4	25	theme	strong	770:775	arg1	interaction					792:802	a strong interchelation interaction	768:802	a strong interchelation interaction	768:802	The shift in the MMT band at 1030 cm-1 to lower wavenumber indicated a strong interchelation interaction in the composite.
32781125	3	26	from	1606 cm-1	625:633	arg1	composites					642:651	the composites	638:651	the composites	638:651	FTIR results revealed the emergence of vibrational bands in the range of 1622 to 1606 cm-1 in the composites spectra indicating the grafting of APA to CS.
32781125	2	27	theme	light	441:445	arg1	DLS					459:461	DLS	459:461	DLS	459:461	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	2	27	theme	light	441:445	arg1	scattering					447:456	dynamic light scattering	433:456	dynamic light scattering (DLS)	433:462	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	6	28	theme	composite	1074:1082	arg1	stability					1057:1065	The thermal stability	1045:1065	The thermal stability of the composite	1045:1082	The thermal stability of the composite increased at lower irradiation dose and higher content of APA.
32781125	0	29	from	composite	56:64	arg1	flocculation					111:122	river water flocculation	99:122	river water flocculation	99:122	Synthesis of montmorillonite/chitosan/ammonium acrylate composite and its potential application in river water flocculation.
32781125	8	30	theme	soft	1370:1373	arg1	nature					1375:1380	the soft nature	1366:1380	the soft nature of CS-g-APA	1366:1392	SEM showed the imperfect microsphere morphology of the composite due to the soft nature of CS-g-APA.
32781125	9	31	from	performance	1412:1422	arg1	water					1451:1455	river water	1445:1455	river water	1445:1455	The flocculation performance of the composites on river water was tested by jar test and compared with alum.
32781125	12	32	theme	composite	1828:1836	arg1	20 mg/50 ml					1809:1819	20 mg/50 ml	1809:1819	20 mg/50 ml of the composite	1809:1836	A dose of 20 mg/50 ml of the composite was sufficient to completely remove MB from a solution with an initial concentration of 50 mg/l.
32781125	4	33	from	1030 cm-1	728:736	arg1	shift					703:707	The shift	699:707	The shift in the MMT band at 1030 cm-1 to lower wavenumber	699:756	The shift in the MMT band at 1030 cm-1 to lower wavenumber indicated a strong interchelation interaction in the composite.
32781125	2	34	theme	scanning	465:472	arg1	SEM					495:497	SEM	495:497	SEM	495:497	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	2	34	theme	scanning	465:472	arg1	microscopy					483:492	scanning electron microscopy	465:492	scanning electron microscopy (SEM)	465:498	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	4	35	theme	lower	741:745	arg1	wavenumber					747:756	lower wavenumber	741:756	lower wavenumber	741:756	The shift in the MMT band at 1030 cm-1 to lower wavenumber indicated a strong interchelation interaction in the composite.
32781125	0	36	theme	montmorillonite/chitosan/ammonium	13:45	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of montmorillonite/chitosan/ammonium	0:45	Synthesis of montmorillonite/chitosan/ammonium acrylate composite and its potential application in river water flocculation.
32781125	6	37	theme	thermal	1049:1055	arg1	stability					1057:1065	The thermal stability	1045:1065	The thermal stability of the composite	1045:1082	The thermal stability of the composite increased at lower irradiation dose and higher content of APA.
32781125	9	38	theme	river	1445:1449	arg1	water					1451:1455	river water	1445:1455	river water	1445:1455	The flocculation performance of the composites on river water was tested by jar test and compared with alum.
32781125	3	39	theme	spectra	653:659	arg1	range					608:612	the range	604:612	the range of 1622 to 1606 cm-1 in the composites spectra	604:659	FTIR results revealed the emergence of vibrational bands in the range of 1622 to 1606 cm-1 in the composites spectra indicating the grafting of APA to CS.
32781125	2	40	theme	thermal	397:403	arg1	analysis					417:424	thermal gravimetric analysis	397:424	thermal gravimetric analysis (TGA)	397:430	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	2	40	theme	thermal	397:403	arg1	TGA					427:429	TGA	427:429	TGA	427:429	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	7	41	theme	particles	1283:1291	arg1	swelling					1257:1264	the swelling	1253:1264	the swelling of the composite particles	1253:1291	TEM images indicated that MMT particles were completely covered with CS-g-APA matrix and the DLS revealed the swelling of the composite particles.
32781125	5	42	theme	interchelated-exfoliated	981:1004	arg1	nanostructure					1006:1018	the interchelated-exfoliated nanostructure	977:1018	the interchelated-exfoliated nanostructure of MMT by CS-APA chains	977:1042	XRD results showed a shift in the MMT diffraction peak from 2θ = 7° to 5.3° with an increased interplanar spacing from 13.36 Å to 16.61 Å, which indicated the interchelated-exfoliated nanostructure of MMT by CS-APA chains.
32781125	5	43	dep	showed	834:839	arg1	indicated					967:975	indicated	967:975	indicated the interchelated-exfoliated nanostructure of MMT by CS-APA chains	967:1042	XRD results showed a shift in the MMT diffraction peak from 2θ = 7° to 5.3° with an increased interplanar spacing from 13.36 Å to 16.61 Å, which indicated the interchelated-exfoliated nanostructure of MMT by CS-APA chains.
32781125	1	44	theme	gamma	235:239	arg1	irradiation					241:251	gamma irradiation	235:251	gamma irradiation (3-7 kGy) induced polymerization	235:284	Composites based on montmorillonite/chitosan-grafted-ammonium polyacrylate (MMT/CS-g-APA) were synthesized by gamma irradiation (3-7 kGy) induced polymerization.
32781125	1	44	theme	gamma	235:239	arg1	3-7 kGy					254:260	3-7 kGy	254:260	3-7 kGy	254:260	Composites based on montmorillonite/chitosan-grafted-ammonium polyacrylate (MMT/CS-g-APA) were synthesized by gamma irradiation (3-7 kGy) induced polymerization.
32781125	6	45	theme	irradiation	1103:1113	arg1	dose					1115:1118	lower irradiation dose	1097:1118	lower irradiation dose	1097:1118	The thermal stability of the composite increased at lower irradiation dose and higher content of APA.
32781125	10	46	theme	equal	1532:1536	arg1	ratios					1538:1543	equal ratios	1532:1543	equal ratios of CS and APA and irradiated at 3 kGy	1532:1581	The composite prepared with equal ratios of CS and APA and irradiated at 3 kGy approached the flocculation efficiency of alum with a remaining turbidity % of 9 and 8% for the composite and alum respectively.
32781125	7	47	theme	CS-g-APA	1216:1223	arg1	matrix					1225:1230	CS-g-APA matrix	1216:1230	CS-g-APA matrix	1216:1230	TEM images indicated that MMT particles were completely covered with CS-g-APA matrix and the DLS revealed the swelling of the composite particles.
32781125	3	48	from	composites	642:651	arg1	1606 cm-1					625:633	1606 cm-1	625:633	1606 cm-1	625:633	FTIR results revealed the emergence of vibrational bands in the range of 1622 to 1606 cm-1 in the composites spectra indicating the grafting of APA to CS.
32781125	3	49	theme	bands	595:599	arg1	emergence					570:578	the emergence	566:578	the emergence of vibrational bands	566:599	FTIR results revealed the emergence of vibrational bands in the range of 1622 to 1606 cm-1 in the composites spectra indicating the grafting of APA to CS.
32781125	5	50	theme	diffraction	860:870	arg1	peak					872:875	the MMT diffraction peak	852:875	the MMT diffraction peak	852:875	XRD results showed a shift in the MMT diffraction peak from 2θ = 7° to 5.3° with an increased interplanar spacing from 13.36 Å to 16.61 Å, which indicated the interchelated-exfoliated nanostructure of MMT by CS-APA chains.
32781125	2	51	theme	X-ray	372:376	arg1	XRD					391:393	XRD	391:393	XRD	391:393	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	2	51	theme	X-ray	372:376	arg1	diffraction					378:388	X-ray diffraction	372:388	X-ray diffraction (XRD)	372:394	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	12	52	theme	initial	1901:1907	arg1	concentration					1909:1921	an initial concentration	1898:1921	an initial concentration of 50 mg/l	1898:1932	A dose of 20 mg/50 ml of the composite was sufficient to completely remove MB from a solution with an initial concentration of 50 mg/l.
32781125	6	53	theme	APA	1142:1144	arg1	dose					1115:1118	lower irradiation dose	1097:1118	lower irradiation dose	1097:1118	The thermal stability of the composite increased at lower irradiation dose and higher content of APA.
32781125	6	53	theme	APA	1142:1144	arg1	content					1131:1137	higher content	1124:1137	higher content of APA	1124:1144	The thermal stability of the composite increased at lower irradiation dose and higher content of APA.
32781125	9	54	theme	jar	1471:1473	arg1	test					1475:1478	jar test	1471:1478	jar test	1471:1478	The flocculation performance of the composites on river water was tested by jar test and compared with alum.
32781125	10	55	theme	flocculation	1598:1609	arg1	efficiency					1611:1620	the flocculation efficiency	1594:1620	the flocculation efficiency of alum	1594:1628	The composite prepared with equal ratios of CS and APA and irradiated at 3 kGy approached the flocculation efficiency of alum with a remaining turbidity % of 9 and 8% for the composite and alum respectively.
32781125	11	56	theme	blue	1777:1780	arg1	methylene					1767:1775	methylene blue	1767:1780	methylene blue (MB)	1767:1785	Furthermore, the composite was used for the removal of methylene blue (MB) from water.
32781125	11	56	theme	blue	1777:1780	arg1	MB					1783:1784	MB	1783:1784	MB	1783:1784	Furthermore, the composite was used for the removal of methylene blue (MB) from water.
32781125	2	57	theme	infrared	342:349	arg1	FTIR					365:368	FTIR	365:368	FTIR	365:368	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	2	57	theme	infrared	342:349	arg1	spectroscopy					351:362	Fourier-transform infrared spectroscopy	324:362	Fourier-transform infrared spectroscopy (FTIR)	324:369	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	5	58	theme	increased	906:914	arg1	spacing					928:934	an increased interplanar spacing	903:934	an increased interplanar spacing from 13.36 Å to 16.61 Å	903:958	XRD results showed a shift in the MMT diffraction peak from 2θ = 7° to 5.3° with an increased interplanar spacing from 13.36 Å to 16.61 Å, which indicated the interchelated-exfoliated nanostructure of MMT by CS-APA chains.
32781125	4	59	theme	MMT	716:718	arg1	band					720:723	the MMT band	712:723	the MMT band	712:723	The shift in the MMT band at 1030 cm-1 to lower wavenumber indicated a strong interchelation interaction in the composite.
32781125	8	60	theme	imperfect	1309:1317	arg1	morphology					1331:1340	the imperfect microsphere morphology	1305:1340	the imperfect microsphere morphology of the composite due to the soft nature of CS-g-APA	1305:1392	SEM showed the imperfect microsphere morphology of the composite due to the soft nature of CS-g-APA.
32781125	0	61	theme	water	105:109	arg1	flocculation					111:122	river water flocculation	99:122	river water flocculation	99:122	Synthesis of montmorillonite/chitosan/ammonium acrylate composite and its potential application in river water flocculation.
32781125	2	62	theme	transmission	504:515	arg1	microscopy					526:535	transmission electron microscopy	504:535	transmission electron microscopy (TEM)	504:541	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	2	62	theme	transmission	504:515	arg1	TEM					538:540	TEM	538:540	TEM	538:540	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	5	63	from	2θ = 7°	882:888	arg1	shift					843:847	a shift	841:847	a shift in the MMT diffraction peak from 2θ = 7° to 5.3°	841:896	XRD results showed a shift in the MMT diffraction peak from 2θ = 7° to 5.3° with an increased interplanar spacing from 13.36 Å to 16.61 Å, which indicated the interchelated-exfoliated nanostructure of MMT by CS-APA chains.
32781125	8	64	theme	due	1359:1361	arg1	composite					1349:1357	the composite	1345:1357	the composite due to the soft nature of CS-g-APA	1345:1392	SEM showed the imperfect microsphere morphology of the composite due to the soft nature of CS-g-APA.
32781125	2	65	theme	dynamic	433:439	arg1	DLS					459:461	DLS	459:461	DLS	459:461	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	2	65	theme	dynamic	433:439	arg1	scattering					447:456	dynamic light scattering	433:456	dynamic light scattering (DLS)	433:462	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	7	66	theme	MMT	1173:1175	arg1	particles					1177:1185	MMT particles	1173:1185	MMT particles	1173:1185	TEM images indicated that MMT particles were completely covered with CS-g-APA matrix and the DLS revealed the swelling of the composite particles.
32781125	2	67	theme	electron	474:481	arg1	SEM					495:497	SEM	495:497	SEM	495:497	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	2	67	theme	electron	474:481	arg1	microscopy					483:492	scanning electron microscopy	465:492	scanning electron microscopy (SEM)	465:498	The composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
32781125	11	68	from	water	1792:1796	arg1	removal					1756:1762	the removal	1752:1762	the removal of methylene blue (MB) from water	1752:1796	Furthermore, the composite was used for the removal of methylene blue (MB) from water.
32781125	10	69	theme	alum	1625:1628	arg1	efficiency					1611:1620	the flocculation efficiency	1594:1620	the flocculation efficiency of alum	1594:1628	The composite prepared with equal ratios of CS and APA and irradiated at 3 kGy approached the flocculation efficiency of alum with a remaining turbidity % of 9 and 8% for the composite and alum respectively.
32781125	5	70	theme	XRD	822:824	arg1	results					826:832	XRD results	822:832	XRD results	822:832	XRD results showed a shift in the MMT diffraction peak from 2θ = 7° to 5.3° with an increased interplanar spacing from 13.36 Å to 16.61 Å, which indicated the interchelated-exfoliated nanostructure of MMT by CS-APA chains.
32781125	7	71	theme	TEM	1147:1149	arg1	images					1151:1156	TEM images	1147:1156	TEM images	1147:1156	TEM images indicated that MMT particles were completely covered with CS-g-APA matrix and the DLS revealed the swelling of the composite particles.
32781125	12	72	theme	20 mg/50 ml	1809:1819	arg1	dose					1801:1804	A dose	1799:1804	A dose of 20 mg/50 ml of the composite	1799:1836	A dose of 20 mg/50 ml of the composite was sufficient to completely remove MB from a solution with an initial concentration of 50 mg/l.
32781125	12	72	theme	20 mg/50 ml	1809:1819	arg1	sufficient					1842:1851	sufficient	1842:1851	sufficient	1842:1851	A dose of 20 mg/50 ml of the composite was sufficient to completely remove MB from a solution with an initial concentration of 50 mg/l.
32781125	5	73	theme	MMT	1023:1025	arg1	nanostructure					1006:1018	the interchelated-exfoliated nanostructure	977:1018	the interchelated-exfoliated nanostructure of MMT by CS-APA chains	977:1042	XRD results showed a shift in the MMT diffraction peak from 2θ = 7° to 5.3° with an increased interplanar spacing from 13.36 Å to 16.61 Å, which indicated the interchelated-exfoliated nanostructure of MMT by CS-APA chains.
32781125	10	74	theme	remaining	1637:1645	arg1	%					1669:1669	9 and 8%	1662:1669	9 and 8% for the composite and alum respectively	1662:1709	The composite prepared with equal ratios of CS and APA and irradiated at 3 kGy approached the flocculation efficiency of alum with a remaining turbidity % of 9 and 8% for the composite and alum respectively.
32781125	10	74	theme	remaining	1637:1645	arg1	%					1657:1657	a remaining turbidity %	1635:1657	a remaining turbidity % of 9 and 8% for the composite and alum respectively	1635:1709	The composite prepared with equal ratios of CS and APA and irradiated at 3 kGy approached the flocculation efficiency of alum with a remaining turbidity % of 9 and 8% for the composite and alum respectively.
32781125	8	75	theme	CS-g-APA	1385:1392	arg1	nature					1375:1380	the soft nature	1366:1380	the soft nature of CS-g-APA	1366:1392	SEM showed the imperfect microsphere morphology of the composite due to the soft nature of CS-g-APA.
32781125	10	76	theme	turbidity	1647:1655	arg1	%					1669:1669	9 and 8%	1662:1669	9 and 8% for the composite and alum respectively	1662:1709	The composite prepared with equal ratios of CS and APA and irradiated at 3 kGy approached the flocculation efficiency of alum with a remaining turbidity % of 9 and 8% for the composite and alum respectively.
32781125	10	76	theme	turbidity	1647:1655	arg1	%					1657:1657	a remaining turbidity %	1635:1657	a remaining turbidity % of 9 and 8% for the composite and alum respectively	1635:1709	The composite prepared with equal ratios of CS and APA and irradiated at 3 kGy approached the flocculation efficiency of alum with a remaining turbidity % of 9 and 8% for the composite and alum respectively.
32781125	0	77	theme	potential	74:82	arg1	application					84:94	its potential application	70:94	its potential application in river water flocculation	70:122	Synthesis of montmorillonite/chitosan/ammonium acrylate composite and its potential application in river water flocculation.
32781125	10	78	from	ratios	1538:1543	arg1	3 kGy					1577:1581	3 kGy	1577:1581	3 kGy	1577:1581	The composite prepared with equal ratios of CS and APA and irradiated at 3 kGy approached the flocculation efficiency of alum with a remaining turbidity % of 9 and 8% for the composite and alum respectively.
32781125	5	79	theme	CS-APA	1030:1035	arg1	chains					1037:1042	CS-APA chains	1030:1042	CS-APA chains	1030:1042	XRD results showed a shift in the MMT diffraction peak from 2θ = 7° to 5.3° with an increased interplanar spacing from 13.36 Å to 16.61 Å, which indicated the interchelated-exfoliated nanostructure of MMT by CS-APA chains.
32781125	7	80	theme	composite	1273:1281	arg1	particles					1283:1291	the composite particles	1269:1291	the composite particles	1269:1291	TEM images indicated that MMT particles were completely covered with CS-g-APA matrix and the DLS revealed the swelling of the composite particles.
32781125	3	81	theme	APA	688:690	arg1	grafting					676:683	the grafting	672:683	the grafting of APA to CS	672:696	FTIR results revealed the emergence of vibrational bands in the range of 1622 to 1606 cm-1 in the composites spectra indicating the grafting of APA to CS.
32781125	10	82	theme	%	1669:1669	arg1	%					1669:1669	9 and 8%	1662:1669	9 and 8% for the composite and alum respectively	1662:1709	The composite prepared with equal ratios of CS and APA and irradiated at 3 kGy approached the flocculation efficiency of alum with a remaining turbidity % of 9 and 8% for the composite and alum respectively.
32781125	10	82	theme	%	1669:1669	arg1	%					1657:1657	a remaining turbidity %	1635:1657	a remaining turbidity % of 9 and 8% for the composite and alum respectively	1635:1709	The composite prepared with equal ratios of CS and APA and irradiated at 3 kGy approached the flocculation efficiency of alum with a remaining turbidity % of 9 and 8% for the composite and alum respectively.
32781125	11	83	used	used	1743:1746	arg2	composite					1729:1737	the composite	1725:1737	the composite	1725:1737	Furthermore, the composite was used for the removal of methylene blue (MB) from water.
32781125	6	84	theme	higher	1124:1129	arg1	content					1131:1137	higher content	1124:1137	higher content of APA	1124:1144	The thermal stability of the composite increased at lower irradiation dose and higher content of APA.
32781125	4	85	theme	interchelation	777:790	arg1	interaction					792:802	a strong interchelation interaction	768:802	a strong interchelation interaction	768:802	The shift in the MMT band at 1030 cm-1 to lower wavenumber indicated a strong interchelation interaction in the composite.
32781125	3	86	theme	FTIR	544:547	arg1	results					549:555	FTIR results	544:555	FTIR results	544:555	FTIR results revealed the emergence of vibrational bands in the range of 1622 to 1606 cm-1 in the composites spectra indicating the grafting of APA to CS.
32781125	4	87	from	shift	703:707	arg1	band					720:723	the MMT band	712:723	the MMT band	712:723	The shift in the MMT band at 1030 cm-1 to lower wavenumber indicated a strong interchelation interaction in the composite.
32781125	9	88	theme	flocculation	1399:1410	arg1	performance					1412:1422	The flocculation performance	1395:1422	The flocculation performance of the composites on river water	1395:1455	The flocculation performance of the composites on river water was tested by jar test and compared with alum.
33493604	7	0	theme	NiMPs	997:1001	arg1	system					1014:1019	The NPs-in-MPs (NiMPs) composite system	981:1019	The NPs-in-MPs (NiMPs) composite system	981:1019	The NPs-in-MPs (NiMPs) composite system provided sustained release of NPs, protected siRNA against nuclease degradation in the serum, and could readily cross the cellular membrane.
33493604	11	1	theme	siRNA	1485:1489	arg1	NiMPs					1453:1457	NiMPs	1453:1457	NiMPs	1453:1457	Therefore, NiMPs are a promising and novel siRNA delivery vehicle for RA therapy.
33493604	11	1	theme	siRNA	1485:1489	arg1	vehicle					1500:1506	a promising and novel siRNA delivery vehicle	1463:1506	a promising and novel siRNA delivery vehicle for RA therapy	1463:1521	Therefore, NiMPs are a promising and novel siRNA delivery vehicle for RA therapy.
33493604	1	2	theme	synovial	245:252	arg1	macrophages					254:264	the synovial macrophages	241:264	the synovial macrophages of patients with rheumatoid arthritis (RA)	241:307	Myeloid cell leukemia-1 (Mcl-1), a member of the Bcl-2 anti-apoptotic family, is overexpressed in the synovial macrophages of patients with rheumatoid arthritis (RA).
33493604	0	3	theme	rational	93:100	arg1	design					102:107	A rational design	91:107	A rational design for rheumatoid arthritis therapy	91:140	PLGA/PCADK composite microspheres containing hyaluronic acid-chitosan siRNA nanoparticles: A rational design for rheumatoid arthritis therapy.
33493604	11	4	theme	delivery	1491:1498	arg1	NiMPs					1453:1457	NiMPs	1453:1457	NiMPs	1453:1457	Therefore, NiMPs are a promising and novel siRNA delivery vehicle for RA therapy.
33493604	11	4	theme	delivery	1491:1498	arg1	vehicle					1500:1506	a promising and novel siRNA delivery vehicle	1463:1506	a promising and novel siRNA delivery vehicle for RA therapy	1463:1521	Therefore, NiMPs are a promising and novel siRNA delivery vehicle for RA therapy.
33493604	6	5	theme	emulsion	964:971	arg1	method					973:978	the double emulsion method	953:978	the double emulsion method	953:978	Then, HCNPs, as an internal aqueous phase, were loaded into poly (D, L-lactide-co-glycolide) (PLGA) and poly (cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK) MPs using the double emulsion method.
33493604	0	6	theme	rheumatoid	113:122	arg1	arthritis					124:132	rheumatoid arthritis	113:132	rheumatoid arthritis therapy	113:140	PLGA/PCADK composite microspheres containing hyaluronic acid-chitosan siRNA nanoparticles: A rational design for rheumatoid arthritis therapy.
33493604	11	7	theme	RA	1512:1513	arg1	therapy					1515:1521	RA therapy	1512:1521	RA therapy	1512:1521	Therefore, NiMPs are a promising and novel siRNA delivery vehicle for RA therapy.
33493604	7	8	theme	NPs-in-MPs	985:994	arg1	system					1014:1019	The NPs-in-MPs (NiMPs) composite system	981:1019	The NPs-in-MPs (NiMPs) composite system	981:1019	The NPs-in-MPs (NiMPs) composite system provided sustained release of NPs, protected siRNA against nuclease degradation in the serum, and could readily cross the cellular membrane.
33493604	8	9	theme	NiMPs	1206:1210	arg1	advantages					1192:1201	the advantages	1188:1201	the advantages of NiMPs	1188:1210	In addition, we evaluated the advantages of NiMPs in an adjuvant-induced arthritis rat model.
33493604	2	10	theme	Small	310:314	arg1	siRNA					333:337	siRNA	333:337	siRNA	333:337	Small interfering RNA (siRNA) Mcl-1 can induce macrophage apoptosis in the joints and is a potential therapeutic target of RA.
33493604	2	10	theme	Small	310:314	arg1	RNA					328:330	Small interfering RNA	310:330	Small interfering RNA (siRNA) Mcl-1	310:344	Small interfering RNA (siRNA) Mcl-1 can induce macrophage apoptosis in the joints and is a potential therapeutic target of RA.
33493604	9	11	theme	common	1346:1351	arg1	MPs					1353:1355	common MPs	1346:1355	common MPs	1346:1355	Our experimental results demonstrate that NiMPs have greater pharmacodynamic effects than common MPs.
33493604	1	12	theme	patients	269:276	arg1	macrophages					254:264	the synovial macrophages	241:264	the synovial macrophages of patients with rheumatoid arthritis (RA)	241:307	Myeloid cell leukemia-1 (Mcl-1), a member of the Bcl-2 anti-apoptotic family, is overexpressed in the synovial macrophages of patients with rheumatoid arthritis (RA).
33493604	6	13	theme	aqueous	805:811	arg1	phase					813:817	an internal aqueous phase	793:817	an internal aqueous phase	793:817	Then, HCNPs, as an internal aqueous phase, were loaded into poly (D, L-lactide-co-glycolide) (PLGA) and poly (cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK) MPs using the double emulsion method.
33493604	4	14	theme	composite	599:607	arg1	MPs					623:625	MPs	623:625	MPs	623:625	To address these shortcomings, we developed composite microspheres (MPs) loaded with hyaluronic acid (HA)-chitosan (CS) nanoparticles (NPs).
33493604	4	14	theme	composite	599:607	arg1	microspheres					609:620	composite microspheres	599:620	composite microspheres (MPs) loaded with hyaluronic acid (HA)-chitosan (CS) nanoparticles (NPs)	599:693	To address these shortcomings, we developed composite microspheres (MPs) loaded with hyaluronic acid (HA)-chitosan (CS) nanoparticles (NPs).
33493604	6	15	theme	double	957:962	arg1	method					973:978	the double emulsion method	953:978	the double emulsion method	953:978	Then, HCNPs, as an internal aqueous phase, were loaded into poly (D, L-lactide-co-glycolide) (PLGA) and poly (cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK) MPs using the double emulsion method.
33493604	0	16	theme	arthritis	124:132	arg1	therapy					134:140	rheumatoid arthritis therapy	113:140	rheumatoid arthritis therapy	113:140	PLGA/PCADK composite microspheres containing hyaluronic acid-chitosan siRNA nanoparticles: A rational design for rheumatoid arthritis therapy.
33493604	6	17	theme	internal	796:803	arg1	phase					813:817	an internal aqueous phase	793:817	an internal aqueous phase	793:817	Then, HCNPs, as an internal aqueous phase, were loaded into poly (D, L-lactide-co-glycolide) (PLGA) and poly (cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK) MPs using the double emulsion method.
33493604	2	18	dep	potential	401:409	arg1	therapeutic					411:421	therapeutic	411:421	therapeutic	411:421	Small interfering RNA (siRNA) Mcl-1 can induce macrophage apoptosis in the joints and is a potential therapeutic target of RA.
33493604	9	19	contain	have	1304:1307	arg1	NiMPs					1298:1302	NiMPs	1298:1302	NiMPs	1298:1302	Our experimental results demonstrate that NiMPs have greater pharmacodynamic effects than common MPs.
33493604	9	19	contain	have	1304:1307	arg2	effects					1333:1339	greater pharmacodynamic effects	1309:1339	greater pharmacodynamic effects	1309:1339	Our experimental results demonstrate that NiMPs have greater pharmacodynamic effects than common MPs.
33493604	6	20	theme	dimethylene	916:926	arg1	poly					881:884	poly	881:884	poly (cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK)	881:941	Then, HCNPs, as an internal aqueous phase, were loaded into poly (D, L-lactide-co-glycolide) (PLGA) and poly (cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK) MPs using the double emulsion method.
33493604	6	20	theme	dimethylene	916:926	arg1	ketal					928:932	cyclohexane-1,4-diyl acetone dimethylene ketal	887:932	cyclohexane-1,4-diyl acetone dimethylene ketal	887:932	Then, HCNPs, as an internal aqueous phase, were loaded into poly (D, L-lactide-co-glycolide) (PLGA) and poly (cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK) MPs using the double emulsion method.
33493604	9	21	theme	pharmacodynamic	1317:1331	arg1	effects					1333:1339	greater pharmacodynamic effects	1309:1339	greater pharmacodynamic effects	1309:1339	Our experimental results demonstrate that NiMPs have greater pharmacodynamic effects than common MPs.
33493604	0	22	theme	composite	11:19	arg1	microspheres					21:32	PLGA/PCADK composite microspheres	0:32	PLGA/PCADK composite microspheres containing hyaluronic acid-chitosan siRNA nanoparticles	0:88	PLGA/PCADK composite microspheres containing hyaluronic acid-chitosan siRNA nanoparticles: A rational design for rheumatoid arthritis therapy.
33493604	0	23	contain	containing	34:43	arg1	microspheres					21:32	PLGA/PCADK composite microspheres	0:32	PLGA/PCADK composite microspheres containing hyaluronic acid-chitosan siRNA nanoparticles	0:88	PLGA/PCADK composite microspheres containing hyaluronic acid-chitosan siRNA nanoparticles: A rational design for rheumatoid arthritis therapy.
33493604	0	23	contain	containing	34:43	arg2	nanoparticles					76:88	hyaluronic acid-chitosan siRNA nanoparticles	45:88	hyaluronic acid-chitosan siRNA nanoparticles	45:88	PLGA/PCADK composite microspheres containing hyaluronic acid-chitosan siRNA nanoparticles: A rational design for rheumatoid arthritis therapy.
33493604	0	24	dep	design	102:107	arg1	microspheres					21:32	PLGA/PCADK composite microspheres	0:32	PLGA/PCADK composite microspheres containing hyaluronic acid-chitosan siRNA nanoparticles	0:88	PLGA/PCADK composite microspheres containing hyaluronic acid-chitosan siRNA nanoparticles: A rational design for rheumatoid arthritis therapy.
33493604	7	25	theme	NPs	1051:1053	arg1	release					1040:1046	sustained release	1030:1046	sustained release of NPs	1030:1053	The NPs-in-MPs (NiMPs) composite system provided sustained release of NPs, protected siRNA against nuclease degradation in the serum, and could readily cross the cellular membrane.
33493604	4	26	theme	hyaluronic	640:649	arg1	HA					657:658	HA	657:658	HA	657:658	To address these shortcomings, we developed composite microspheres (MPs) loaded with hyaluronic acid (HA)-chitosan (CS) nanoparticles (NPs).
33493604	4	26	theme	hyaluronic	640:649	arg1	acid					651:654	hyaluronic acid	640:654	hyaluronic acid (HA)-chitosan (CS) nanoparticles (NPs)	640:693	To address these shortcomings, we developed composite microspheres (MPs) loaded with hyaluronic acid (HA)-chitosan (CS) nanoparticles (NPs).
33493604	0	27	theme	PLGA/PCADK	0:9	arg1	microspheres					21:32	PLGA/PCADK composite microspheres	0:32	PLGA/PCADK composite microspheres containing hyaluronic acid-chitosan siRNA nanoparticles	0:88	PLGA/PCADK composite microspheres containing hyaluronic acid-chitosan siRNA nanoparticles: A rational design for rheumatoid arthritis therapy.
33493604	7	28	theme	sustained	1030:1038	arg1	release					1040:1046	sustained release	1030:1046	sustained release of NPs	1030:1053	The NPs-in-MPs (NiMPs) composite system provided sustained release of NPs, protected siRNA against nuclease degradation in the serum, and could readily cross the cellular membrane.
33493604	8	29	theme	adjuvant-induced	1218:1233	arg1	model					1249:1253	an adjuvant-induced arthritis rat model	1215:1253	an adjuvant-induced arthritis rat model	1215:1253	In addition, we evaluated the advantages of NiMPs in an adjuvant-induced arthritis rat model.
33493604	0	30	theme	siRNA	70:74	arg1	nanoparticles					76:88	hyaluronic acid-chitosan siRNA nanoparticles	45:88	hyaluronic acid-chitosan siRNA nanoparticles	45:88	PLGA/PCADK composite microspheres containing hyaluronic acid-chitosan siRNA nanoparticles: A rational design for rheumatoid arthritis therapy.
33493604	2	31	theme	macrophage	357:366	arg1	apoptosis					368:376	macrophage apoptosis	357:376	macrophage apoptosis	357:376	Small interfering RNA (siRNA) Mcl-1 can induce macrophage apoptosis in the joints and is a potential therapeutic target of RA.
33493604	1	32	theme	rheumatoid	283:292	arg1	arthritis					294:302	rheumatoid arthritis	283:302	rheumatoid arthritis (RA)	283:307	Myeloid cell leukemia-1 (Mcl-1), a member of the Bcl-2 anti-apoptotic family, is overexpressed in the synovial macrophages of patients with rheumatoid arthritis (RA).
33493604	1	32	theme	rheumatoid	283:292	arg1	RA					305:306	RA	305:306	RA	305:306	Myeloid cell leukemia-1 (Mcl-1), a member of the Bcl-2 anti-apoptotic family, is overexpressed in the synovial macrophages of patients with rheumatoid arthritis (RA).
33493604	9	33	theme	greater	1309:1315	arg1	effects					1333:1339	greater pharmacodynamic effects	1309:1339	greater pharmacodynamic effects	1309:1339	Our experimental results demonstrate that NiMPs have greater pharmacodynamic effects than common MPs.
33493604	1	34	theme	Bcl-2	192:196	arg1	family					213:218	the Bcl-2 anti-apoptotic family	188:218	the Bcl-2 anti-apoptotic family	188:218	Myeloid cell leukemia-1 (Mcl-1), a member of the Bcl-2 anti-apoptotic family, is overexpressed in the synovial macrophages of patients with rheumatoid arthritis (RA).
33493604	2	35	theme	potential	401:409	arg1	target					423:428	a potential therapeutic target	399:428	a potential therapeutic target of RA	399:434	Small interfering RNA (siRNA) Mcl-1 can induce macrophage apoptosis in the joints and is a potential therapeutic target of RA.
33493604	8	36	theme	rat	1245:1247	arg1	model					1249:1253	an adjuvant-induced arthritis rat model	1215:1253	an adjuvant-induced arthritis rat model	1215:1253	In addition, we evaluated the advantages of NiMPs in an adjuvant-induced arthritis rat model.
33493604	1	37	theme	anti-apoptotic	198:211	arg1	family					213:218	the Bcl-2 anti-apoptotic family	188:218	the Bcl-2 anti-apoptotic family	188:218	Myeloid cell leukemia-1 (Mcl-1), a member of the Bcl-2 anti-apoptotic family, is overexpressed in the synovial macrophages of patients with rheumatoid arthritis (RA).
33493604	0	38	theme	acid-chitosan	56:68	arg1	nanoparticles					76:88	hyaluronic acid-chitosan siRNA nanoparticles	45:88	hyaluronic acid-chitosan siRNA nanoparticles	45:88	PLGA/PCADK composite microspheres containing hyaluronic acid-chitosan siRNA nanoparticles: A rational design for rheumatoid arthritis therapy.
33493604	3	39	theme	siRNA	470:474	arg1	application					455:465	the application	451:465	the application of siRNA	451:474	Nevertheless, the application of siRNA is limited owing to its instability and susceptibility to degradation in vivo.
33493604	6	40	dep	poly	837:840	arg1	D					843:843	D	843:843	D	843:843	Then, HCNPs, as an internal aqueous phase, were loaded into poly (D, L-lactide-co-glycolide) (PLGA) and poly (cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK) MPs using the double emulsion method.
33493604	6	40	dep	poly	837:840	arg1	L-lactide-co-glycolide					846:867	L-lactide-co-glycolide	846:867	L-lactide-co-glycolide	846:867	Then, HCNPs, as an internal aqueous phase, were loaded into poly (D, L-lactide-co-glycolide) (PLGA) and poly (cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK) MPs using the double emulsion method.
33493604	6	40	dep	poly	837:840	arg1	MPs					943:945	MPs	943:945	MPs	943:945	Then, HCNPs, as an internal aqueous phase, were loaded into poly (D, L-lactide-co-glycolide) (PLGA) and poly (cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK) MPs using the double emulsion method.
33493604	1	41	theme	family	213:218	arg1	leukemia-1					156:165	Myeloid cell leukemia-1	143:165	Myeloid cell leukemia-1 (Mcl-1)	143:173	Myeloid cell leukemia-1 (Mcl-1), a member of the Bcl-2 anti-apoptotic family, is overexpressed in the synovial macrophages of patients with rheumatoid arthritis (RA).
33493604	1	41	theme	family	213:218	arg1	member					178:183	a member	176:183	a member of the Bcl-2 anti-apoptotic family	176:218	Myeloid cell leukemia-1 (Mcl-1), a member of the Bcl-2 anti-apoptotic family, is overexpressed in the synovial macrophages of patients with rheumatoid arthritis (RA).
33493604	0	42	theme	hyaluronic	45:54	arg1	nanoparticles					76:88	hyaluronic acid-chitosan siRNA nanoparticles	45:88	hyaluronic acid-chitosan siRNA nanoparticles	45:88	PLGA/PCADK composite microspheres containing hyaluronic acid-chitosan siRNA nanoparticles: A rational design for rheumatoid arthritis therapy.
33493604	5	43	theme	HA-CS/siRNA	718:728	arg1	NPs					730:732	HA-CS/siRNA NPs	718:732	HA-CS/siRNA NPs (HCNPs)	718:740	First, we synthesized HA-CS/siRNA NPs (HCNPs) using ionotropic gelation process.
33493604	5	43	theme	HA-CS/siRNA	718:728	arg1	HCNPs					735:739	HCNPs	735:739	HCNPs	735:739	First, we synthesized HA-CS/siRNA NPs (HCNPs) using ionotropic gelation process.
33493604	6	44	theme	cyclohexane-1,4-diyl	887:906	arg1	poly					881:884	poly	881:884	poly (cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK)	881:941	Then, HCNPs, as an internal aqueous phase, were loaded into poly (D, L-lactide-co-glycolide) (PLGA) and poly (cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK) MPs using the double emulsion method.
33493604	6	44	theme	cyclohexane-1,4-diyl	887:906	arg1	ketal					928:932	cyclohexane-1,4-diyl acetone dimethylene ketal	887:932	cyclohexane-1,4-diyl acetone dimethylene ketal	887:932	Then, HCNPs, as an internal aqueous phase, were loaded into poly (D, L-lactide-co-glycolide) (PLGA) and poly (cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK) MPs using the double emulsion method.
33493604	11	45	theme	promising	1465:1473	arg1	NiMPs					1453:1457	NiMPs	1453:1457	NiMPs	1453:1457	Therefore, NiMPs are a promising and novel siRNA delivery vehicle for RA therapy.
33493604	11	45	theme	promising	1465:1473	arg1	vehicle					1500:1506	a promising and novel siRNA delivery vehicle	1463:1506	a promising and novel siRNA delivery vehicle for RA therapy	1463:1521	Therefore, NiMPs are a promising and novel siRNA delivery vehicle for RA therapy.
33493604	7	46	theme	composite	1004:1012	arg1	system					1014:1019	The NPs-in-MPs (NiMPs) composite system	981:1019	The NPs-in-MPs (NiMPs) composite system	981:1019	The NPs-in-MPs (NiMPs) composite system provided sustained release of NPs, protected siRNA against nuclease degradation in the serum, and could readily cross the cellular membrane.
33493604	8	47	theme	arthritis	1235:1243	arg1	model					1249:1253	an adjuvant-induced arthritis rat model	1215:1253	an adjuvant-induced arthritis rat model	1215:1253	In addition, we evaluated the advantages of NiMPs in an adjuvant-induced arthritis rat model.
33493604	1	48	with	patients	269:276	arg1	arthritis					294:302	rheumatoid arthritis	283:302	rheumatoid arthritis (RA)	283:307	Myeloid cell leukemia-1 (Mcl-1), a member of the Bcl-2 anti-apoptotic family, is overexpressed in the synovial macrophages of patients with rheumatoid arthritis (RA).
33493604	1	48	with	patients	269:276	arg1	RA					305:306	RA	305:306	RA	305:306	Myeloid cell leukemia-1 (Mcl-1), a member of the Bcl-2 anti-apoptotic family, is overexpressed in the synovial macrophages of patients with rheumatoid arthritis (RA).
33493604	7	49	theme	cellular	1143:1150	arg1	membrane					1152:1159	the cellular membrane	1139:1159	the cellular membrane	1139:1159	The NPs-in-MPs (NiMPs) composite system provided sustained release of NPs, protected siRNA against nuclease degradation in the serum, and could readily cross the cellular membrane.
33493604	2	50	theme	RA	433:434	arg1	target					423:428	a potential therapeutic target	399:428	a potential therapeutic target of RA	399:434	Small interfering RNA (siRNA) Mcl-1 can induce macrophage apoptosis in the joints and is a potential therapeutic target of RA.
33493604	7	51	from	degradation	1089:1099	arg1	serum					1108:1112	the serum	1104:1112	the serum	1104:1112	The NPs-in-MPs (NiMPs) composite system provided sustained release of NPs, protected siRNA against nuclease degradation in the serum, and could readily cross the cellular membrane.
33493604	7	52	theme	nuclease	1080:1087	arg1	degradation					1089:1099	nuclease degradation	1080:1099	nuclease degradation in the serum	1080:1112	The NPs-in-MPs (NiMPs) composite system provided sustained release of NPs, protected siRNA against nuclease degradation in the serum, and could readily cross the cellular membrane.
33493604	1	53	theme	Myeloid	143:149	arg1	Mcl-1					168:172	Mcl-1	168:172	Mcl-1	168:172	Myeloid cell leukemia-1 (Mcl-1), a member of the Bcl-2 anti-apoptotic family, is overexpressed in the synovial macrophages of patients with rheumatoid arthritis (RA).
33493604	1	53	theme	Myeloid	143:149	arg1	leukemia-1					156:165	Myeloid cell leukemia-1	143:165	Myeloid cell leukemia-1 (Mcl-1)	143:173	Myeloid cell leukemia-1 (Mcl-1), a member of the Bcl-2 anti-apoptotic family, is overexpressed in the synovial macrophages of patients with rheumatoid arthritis (RA).
33493604	1	53	theme	Myeloid	143:149	arg1	member					178:183	a member	176:183	a member of the Bcl-2 anti-apoptotic family	176:218	Myeloid cell leukemia-1 (Mcl-1), a member of the Bcl-2 anti-apoptotic family, is overexpressed in the synovial macrophages of patients with rheumatoid arthritis (RA).
33493604	1	54	theme	cell	151:154	arg1	Mcl-1					168:172	Mcl-1	168:172	Mcl-1	168:172	Myeloid cell leukemia-1 (Mcl-1), a member of the Bcl-2 anti-apoptotic family, is overexpressed in the synovial macrophages of patients with rheumatoid arthritis (RA).
33493604	1	54	theme	cell	151:154	arg1	leukemia-1					156:165	Myeloid cell leukemia-1	143:165	Myeloid cell leukemia-1 (Mcl-1)	143:173	Myeloid cell leukemia-1 (Mcl-1), a member of the Bcl-2 anti-apoptotic family, is overexpressed in the synovial macrophages of patients with rheumatoid arthritis (RA).
33493604	1	54	theme	cell	151:154	arg1	member					178:183	a member	176:183	a member of the Bcl-2 anti-apoptotic family	176:218	Myeloid cell leukemia-1 (Mcl-1), a member of the Bcl-2 anti-apoptotic family, is overexpressed in the synovial macrophages of patients with rheumatoid arthritis (RA).
33493604	9	55	theme	experimental	1260:1271	arg1	results					1273:1279	Our experimental results	1256:1279	Our experimental results	1256:1279	Our experimental results demonstrate that NiMPs have greater pharmacodynamic effects than common MPs.
33493604	2	56	theme	interfering	316:326	arg1	siRNA					333:337	siRNA	333:337	siRNA	333:337	Small interfering RNA (siRNA) Mcl-1 can induce macrophage apoptosis in the joints and is a potential therapeutic target of RA.
33493604	2	56	theme	interfering	316:326	arg1	RNA					328:330	Small interfering RNA	310:330	Small interfering RNA (siRNA) Mcl-1	310:344	Small interfering RNA (siRNA) Mcl-1 can induce macrophage apoptosis in the joints and is a potential therapeutic target of RA.
33493604	2	57	theme	RNA	328:330	arg1	Mcl-1					340:344	Small interfering RNA (siRNA) Mcl-1	310:344	Small interfering RNA (siRNA) Mcl-1	310:344	Small interfering RNA (siRNA) Mcl-1 can induce macrophage apoptosis in the joints and is a potential therapeutic target of RA.
33493604	10	58	theme	administration	1426:1439	arg1	frequency					1408:1416	the frequency	1404:1416	the frequency of drug administration	1404:1439	Meanwhile, compared with HCNPs, NiMPs reduced the frequency of drug administration.
33493604	5	59	theme	gelation	759:766	arg1	process					768:774	ionotropic gelation process	748:774	ionotropic gelation process	748:774	First, we synthesized HA-CS/siRNA NPs (HCNPs) using ionotropic gelation process.
33493604	6	60	theme	acetone	908:914	arg1	poly					881:884	poly	881:884	poly (cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK)	881:941	Then, HCNPs, as an internal aqueous phase, were loaded into poly (D, L-lactide-co-glycolide) (PLGA) and poly (cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK) MPs using the double emulsion method.
33493604	6	60	theme	acetone	908:914	arg1	ketal					928:932	cyclohexane-1,4-diyl acetone dimethylene ketal	887:932	cyclohexane-1,4-diyl acetone dimethylene ketal	887:932	Then, HCNPs, as an internal aqueous phase, were loaded into poly (D, L-lactide-co-glycolide) (PLGA) and poly (cyclohexane-1,4-diyl acetone dimethylene ketal) (PCADK) MPs using the double emulsion method.
33493604	10	61	theme	drug	1421:1424	arg1	administration					1426:1439	drug administration	1421:1439	drug administration	1421:1439	Meanwhile, compared with HCNPs, NiMPs reduced the frequency of drug administration.
33493604	5	62	theme	ionotropic	748:757	arg1	process					768:774	ionotropic gelation process	748:774	ionotropic gelation process	748:774	First, we synthesized HA-CS/siRNA NPs (HCNPs) using ionotropic gelation process.
33493604	11	63	theme	novel	1479:1483	arg1	NiMPs					1453:1457	NiMPs	1453:1457	NiMPs	1453:1457	Therefore, NiMPs are a promising and novel siRNA delivery vehicle for RA therapy.
33493604	11	63	theme	novel	1479:1483	arg1	vehicle					1500:1506	a promising and novel siRNA delivery vehicle	1463:1506	a promising and novel siRNA delivery vehicle for RA therapy	1463:1521	Therefore, NiMPs are a promising and novel siRNA delivery vehicle for RA therapy.
33493604	4	64	theme	acid	651:654	arg1	CS					671:672	CS	671:672	CS	671:672	To address these shortcomings, we developed composite microspheres (MPs) loaded with hyaluronic acid (HA)-chitosan (CS) nanoparticles (NPs).
33493604	4	64	theme	acid	651:654	arg1	-chitosan					660:668	hyaluronic acid (HA)-chitosan	640:668	hyaluronic acid (HA)-chitosan (CS) nanoparticles (NPs)	640:693	To address these shortcomings, we developed composite microspheres (MPs) loaded with hyaluronic acid (HA)-chitosan (CS) nanoparticles (NPs).
33493604	4	65	theme	-chitosan	660:668	arg1	NPs					690:692	NPs	690:692	NPs	690:692	To address these shortcomings, we developed composite microspheres (MPs) loaded with hyaluronic acid (HA)-chitosan (CS) nanoparticles (NPs).
33493604	4	65	theme	-chitosan	660:668	arg1	nanoparticles					675:687	hyaluronic acid (HA)-chitosan (CS) nanoparticles	640:687	hyaluronic acid (HA)-chitosan (CS) nanoparticles (NPs)	640:693	To address these shortcomings, we developed composite microspheres (MPs) loaded with hyaluronic acid (HA)-chitosan (CS) nanoparticles (NPs).
34339172	4	0	from	reduction	1030:1038	arg1	hemicellulose					1055:1067	(up to 29%) hemicellulose	1043:1067	(up to 29%) hemicellulose	1043:1067	Together with a locally thickened (7-13-fold) cell wall, increased content (up to 58%) of pectin and reduction in (up to 29%) hemicellulose were observed.
34339172	4	0	from	reduction	1030:1038	arg1	%					1052:1052	up to 29%	1044:1052	up to 29%	1044:1052	Together with a locally thickened (7-13-fold) cell wall, increased content (up to 58%) of pectin and reduction in (up to 29%) hemicellulose were observed.
34339172	3	1	theme	morphological	865:877	arg1	changes					897:903	morphological and compositional changes	865:903	morphological and compositional changes in cell walls	865:917	In addition to growth inhibition, morphological and compositional changes in cell walls occurred.
34339172	2	2	theme	tobacco	490:496	arg1	L.					524:525	<i>Nicotiana tabacum</i> L.	499:525	<i>Nicotiana tabacum</i> L.	499:525	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	2	2	theme	tobacco	490:496	arg1	cells					548:552	Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells	467:552	Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells	467:552	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	1	3	theme	plant	357:361	arg1	unknown					439:445	unknown	439:445	unknown	439:445	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>), one of the broadly used rare earth nanoparticles, can interact with plants and possibly cause plant health and environmental impacts, but the plant defense response particularly at the nanoparticle-cell interface is largely unknown.
34339172	1	3	theme	plant	357:361	arg1	response					371:378	the plant defense response	353:378	the plant defense response particularly at the nanoparticle-cell interface	353:426	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>), one of the broadly used rare earth nanoparticles, can interact with plants and possibly cause plant health and environmental impacts, but the plant defense response particularly at the nanoparticle-cell interface is largely unknown.
34339172	8	4	theme	regulatory	1897:1906	arg1	machinery					1908:1916	the plant's cellular regulatory machinery	1876:1916	the plant's cellular regulatory machinery regarding repair and adaptation to nanoparticles like nY<sub>2</sub>O<sub>3</sub>	1876:1998	Our findings indicate that, although nY<sub>2</sub>O<sub>3</sub> generated toxicity on BY-2 cells, it is very likely that during the recovery process cell wall remodeling was initiated to gain resistance to nY<sub>2</sub>O<sub>3</sub> stress, demonstrating the plant's cellular regulatory machinery regarding repair and adaptation to nanoparticles like nY<sub>2</sub>O<sub>3</sub>.
34339172	2	5	theme	nY<sub>2</sub>O<sub>3</sub>	659:685	arg1	%					650:650	42.2%	646:650	42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole	646:828	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	2	5	theme	nY<sub>2</sub>O<sub>3</sub>	659:685	arg1	nY<sub>2</sub>O<sub>3</sub>					659:685	the nY<sub>2</sub>O<sub>3</sub>	655:685	the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole	655:828	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	7	6	theme	vesicle	1494:1500	arg1	trafficking					1502:1512	vesicle trafficking	1494:1512	vesicle trafficking	1494:1512	Interestingly, vesicle trafficking seemed to be activated, enabling the repair and defense against nY<sub>2</sub>O<sub>3</sub> disturbance.
34339172	0	7	theme	Novo</i>	112:119	arg1	Synthesis					121:129	<i>de Novo</i> Synthesis	106:129	<i>de Novo</i> Synthesis	106:129	Cell Walls Are Remodeled to Alleviate nY<sub>2</sub>O<sub>3</sub> Cytotoxicity by Elaborate Regulation of <i>de Novo</i> Synthesis and Vesicular Transport.
34339172	1	8	theme	defense	363:369	arg1	unknown					439:445	unknown	439:445	unknown	439:445	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>), one of the broadly used rare earth nanoparticles, can interact with plants and possibly cause plant health and environmental impacts, but the plant defense response particularly at the nanoparticle-cell interface is largely unknown.
34339172	1	8	theme	defense	363:369	arg1	response					371:378	the plant defense response	353:378	the plant defense response particularly at the nanoparticle-cell interface	353:426	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>), one of the broadly used rare earth nanoparticles, can interact with plants and possibly cause plant health and environmental impacts, but the plant defense response particularly at the nanoparticle-cell interface is largely unknown.
34339172	4	9	dep	29	1050:1051	arg1	to					1047:1048	to	1047:1048	to	1047:1048	Together with a locally thickened (7-13-fold) cell wall, increased content (up to 58%) of pectin and reduction in (up to 29%) hemicellulose were observed.
34339172	0	10	theme	<i>de	106:110	arg1	Synthesis					121:129	<i>de Novo</i> Synthesis	106:129	<i>de Novo</i> Synthesis	106:129	Cell Walls Are Remodeled to Alleviate nY<sub>2</sub>O<sub>3</sub> Cytotoxicity by Elaborate Regulation of <i>de Novo</i> Synthesis and Vesicular Transport.
34339172	8	11	theme	wall	1774:1777	arg1	remodeling					1779:1788	cell wall remodeling	1769:1788	cell wall remodeling	1769:1788	Our findings indicate that, although nY<sub>2</sub>O<sub>3</sub> generated toxicity on BY-2 cells, it is very likely that during the recovery process cell wall remodeling was initiated to gain resistance to nY<sub>2</sub>O<sub>3</sub> stress, demonstrating the plant's cellular regulatory machinery regarding repair and adaptation to nanoparticles like nY<sub>2</sub>O<sub>3</sub>.
34339172	5	12	theme	nY<sub>2</sub>O<sub>3</sub>	1212:1238	arg1	stress					1240:1245	nY<sub>2</sub>O<sub>3</sub> stress	1212:1245	nY<sub>2</sub>O<sub>3</sub> stress	1212:1245	Transcriptome analysis revealed that genes involved in cell wall metabolism and remodeling were highly regulated in response to nY<sub>2</sub>O<sub>3</sub> stress.
34339172	5	13	theme	Transcriptome	1084:1096	arg1	analysis					1098:1105	Transcriptome analysis	1084:1105	Transcriptome analysis	1084:1105	Transcriptome analysis revealed that genes involved in cell wall metabolism and remodeling were highly regulated in response to nY<sub>2</sub>O<sub>3</sub> stress.
34339172	3	14	theme	growth	846:851	arg1	inhibition					853:862	growth inhibition	846:862	growth inhibition	846:862	In addition to growth inhibition, morphological and compositional changes in cell walls occurred.
34339172	8	15	theme	BY-2	1706:1709	arg1	cells					1711:1715	BY-2 cells	1706:1715	BY-2 cells	1706:1715	Our findings indicate that, although nY<sub>2</sub>O<sub>3</sub> generated toxicity on BY-2 cells, it is very likely that during the recovery process cell wall remodeling was initiated to gain resistance to nY<sub>2</sub>O<sub>3</sub> stress, demonstrating the plant's cellular regulatory machinery regarding repair and adaptation to nanoparticles like nY<sub>2</sub>O<sub>3</sub>.
34339172	0	16	theme	Synthesis	121:129	arg1	Regulation					92:101	Elaborate Regulation	82:101	Elaborate Regulation of <i>de Novo</i> Synthesis and Vesicular Transport	82:153	Cell Walls Are Remodeled to Alleviate nY<sub>2</sub>O<sub>3</sub> Cytotoxicity by Elaborate Regulation of <i>de Novo</i> Synthesis and Vesicular Transport.
34339172	4	17	from	content	996:1002	arg1	hemicellulose					1055:1067	(up to 29%) hemicellulose	1043:1067	(up to 29%) hemicellulose	1043:1067	Together with a locally thickened (7-13-fold) cell wall, increased content (up to 58%) of pectin and reduction in (up to 29%) hemicellulose were observed.
34339172	4	17	from	content	996:1002	arg1	%					1052:1052	up to 29%	1044:1052	up to 29%	1044:1052	Together with a locally thickened (7-13-fold) cell wall, increased content (up to 58%) of pectin and reduction in (up to 29%) hemicellulose were observed.
34339172	2	18	theme	Bright	467:472	arg1	L.					524:525	<i>Nicotiana tabacum</i> L.	499:525	<i>Nicotiana tabacum</i> L.	499:525	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	2	18	theme	Bright	467:472	arg1	cells					548:552	Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells	467:552	Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells	467:552	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	2	19	theme	Yellow	474:479	arg1	L.					524:525	<i>Nicotiana tabacum</i> L.	499:525	<i>Nicotiana tabacum</i> L.	499:525	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	2	19	theme	Yellow	474:479	arg1	cells					548:552	Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells	467:552	Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells	467:552	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	5	20	dep	wall	1144:1147	arg1	metabolism					1149:1158	metabolism	1149:1158	metabolism	1149:1158	Transcriptome analysis revealed that genes involved in cell wall metabolism and remodeling were highly regulated in response to nY<sub>2</sub>O<sub>3</sub> stress.
34339172	4	21	dep	thickened	953:961	arg1	7-13-fold					964:972	7-13-fold	964:972	7-13-fold	964:972	Together with a locally thickened (7-13-fold) cell wall, increased content (up to 58%) of pectin and reduction in (up to 29%) hemicellulose were observed.
34339172	0	22	theme	Cell	0:3	arg1	Walls					5:9	Cell Walls	0:9	Cell Walls	0:9	Cell Walls Are Remodeled to Alleviate nY<sub>2</sub>O<sub>3</sub> Cytotoxicity by Elaborate Regulation of <i>de Novo</i> Synthesis and Vesicular Transport.
34339172	6	23	theme	genes	1262:1266	arg1	Expression					1248:1257	Expression	1248:1257	Expression of genes for pectin synthesis and degradation	1248:1303	Expression of genes for pectin synthesis and degradation was up- and down-regulated by 31-78% and 13-42%, respectively, and genes for xyloglucan and pectin modifications were up- and down-regulated by 82% and 81-92%, respectively.
34339172	8	24	theme	cellular	1888:1895	arg1	machinery					1908:1916	the plant's cellular regulatory machinery	1876:1916	the plant's cellular regulatory machinery regarding repair and adaptation to nanoparticles like nY<sub>2</sub>O<sub>3</sub>	1876:1998	Our findings indicate that, although nY<sub>2</sub>O<sub>3</sub> generated toxicity on BY-2 cells, it is very likely that during the recovery process cell wall remodeling was initiated to gain resistance to nY<sub>2</sub>O<sub>3</sub> stress, demonstrating the plant's cellular regulatory machinery regarding repair and adaptation to nanoparticles like nY<sub>2</sub>O<sub>3</sub>.
34339172	0	25	dep	Regulation	92:101	arg1	Elaborate					82:90	Elaborate	82:90	Elaborate	82:90	Cell Walls Are Remodeled to Alleviate nY<sub>2</sub>O<sub>3</sub> Cytotoxicity by Elaborate Regulation of <i>de Novo</i> Synthesis and Vesicular Transport.
34339172	8	26	theme	recovery	1752:1759	arg1	process					1761:1767	the recovery process	1748:1767	the recovery process	1748:1767	Our findings indicate that, although nY<sub>2</sub>O<sub>3</sub> generated toxicity on BY-2 cells, it is very likely that during the recovery process cell wall remodeling was initiated to gain resistance to nY<sub>2</sub>O<sub>3</sub> stress, demonstrating the plant's cellular regulatory machinery regarding repair and adaptation to nanoparticles like nY<sub>2</sub>O<sub>3</sub>.
34339172	0	27	theme	Transport	145:153	arg1	Regulation					92:101	Elaborate Regulation	82:101	Elaborate Regulation of <i>de Novo</i> Synthesis and Vesicular Transport	82:153	Cell Walls Are Remodeled to Alleviate nY<sub>2</sub>O<sub>3</sub> Cytotoxicity by Elaborate Regulation of <i>de Novo</i> Synthesis and Vesicular Transport.
34339172	3	28	from	changes	897:903	arg1	walls					913:917	cell walls	908:917	cell walls	908:917	In addition to growth inhibition, morphological and compositional changes in cell walls occurred.
34339172	1	29	theme	plant	309:313	arg1	health					315:320	plant health and environmental impacts	309:346	health	315:320	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>), one of the broadly used rare earth nanoparticles, can interact with plants and possibly cause plant health and environmental impacts, but the plant defense response particularly at the nanoparticle-cell interface is largely unknown.
34339172	0	30	theme	Vesicular	135:143	arg1	Transport					145:153	Vesicular Transport	135:153	Vesicular Transport	135:153	Cell Walls Are Remodeled to Alleviate nY<sub>2</sub>O<sub>3</sub> Cytotoxicity by Elaborate Regulation of <i>de Novo</i> Synthesis and Vesicular Transport.
34339172	1	31	theme	nanoparticle-cell	400:416	arg1	interface					418:426	the nanoparticle-cell interface	396:426	the nanoparticle-cell interface	396:426	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>), one of the broadly used rare earth nanoparticles, can interact with plants and possibly cause plant health and environmental impacts, but the plant defense response particularly at the nanoparticle-cell interface is largely unknown.
34339172	8	32	theme	cell	1769:1772	arg1	remodeling					1779:1788	cell wall remodeling	1769:1788	cell wall remodeling	1769:1788	Our findings indicate that, although nY<sub>2</sub>O<sub>3</sub> generated toxicity on BY-2 cells, it is very likely that during the recovery process cell wall remodeling was initiated to gain resistance to nY<sub>2</sub>O<sub>3</sub> stress, demonstrating the plant's cellular regulatory machinery regarding repair and adaptation to nanoparticles like nY<sub>2</sub>O<sub>3</sub>.
34339172	4	33	theme	cell	975:978	arg1	wall					980:983	a locally thickened (7-13-fold) cell wall	943:983	a locally thickened (7-13-fold) cell wall	943:983	Together with a locally thickened (7-13-fold) cell wall, increased content (up to 58%) of pectin and reduction in (up to 29%) hemicellulose were observed.
34339172	1	34	theme	used	234:237	arg1	earth					244:248	the broadly used rare earth	222:248	the broadly used rare earth nanoparticles	222:262	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>), one of the broadly used rare earth nanoparticles, can interact with plants and possibly cause plant health and environmental impacts, but the plant defense response particularly at the nanoparticle-cell interface is largely unknown.
34339172	2	35	theme	BY-2	484:487	arg1	L.					524:525	<i>Nicotiana tabacum</i> L.	499:525	<i>Nicotiana tabacum</i> L.	499:525	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	2	35	theme	BY-2	484:487	arg1	cells					548:552	Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells	467:552	Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells	467:552	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	5	36	theme	cell	1139:1142	arg1	wall					1144:1147	cell wall metabolism and remodeling	1139:1173	wall	1144:1147	Transcriptome analysis revealed that genes involved in cell wall metabolism and remodeling were highly regulated in response to nY<sub>2</sub>O<sub>3</sub> stress.
34339172	1	37	from	interface	418:426	arg1	unknown					439:445	unknown	439:445	unknown	439:445	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>), one of the broadly used rare earth nanoparticles, can interact with plants and possibly cause plant health and environmental impacts, but the plant defense response particularly at the nanoparticle-cell interface is largely unknown.
34339172	1	37	from	interface	418:426	arg1	response					371:378	the plant defense response	353:378	the plant defense response particularly at the nanoparticle-cell interface	353:426	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>), one of the broadly used rare earth nanoparticles, can interact with plants and possibly cause plant health and environmental impacts, but the plant defense response particularly at the nanoparticle-cell interface is largely unknown.
34339172	4	38	theme	pectin	1019:1024	arg1	reduction					1030:1038	reduction	1030:1038	reduction in (up to 29%) hemicellulose	1030:1067	Together with a locally thickened (7-13-fold) cell wall, increased content (up to 58%) of pectin and reduction in (up to 29%) hemicellulose were observed.
34339172	4	38	theme	pectin	1019:1024	arg1	%					1013:1013	up to 58%	1005:1013	up to 58%	1005:1013	Together with a locally thickened (7-13-fold) cell wall, increased content (up to 58%) of pectin and reduction in (up to 29%) hemicellulose were observed.
34339172	4	38	theme	pectin	1019:1024	arg1	content					996:1002	increased content	986:1002	increased content (up to 58%) of pectin	986:1024	Together with a locally thickened (7-13-fold) cell wall, increased content (up to 58%) of pectin and reduction in (up to 29%) hemicellulose were observed.
34339172	1	39	theme	environmental	326:338	arg1	impacts					340:346	plant health and environmental impacts	309:346	impacts	340:346	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>), one of the broadly used rare earth nanoparticles, can interact with plants and possibly cause plant health and environmental impacts, but the plant defense response particularly at the nanoparticle-cell interface is largely unknown.
34339172	4	40	dep	58	1011:1012	arg1	to					1008:1009	to	1008:1009	to	1008:1009	Together with a locally thickened (7-13-fold) cell wall, increased content (up to 58%) of pectin and reduction in (up to 29%) hemicellulose were observed.
34339172	4	41	theme	thickened	953:961	arg1	wall					980:983	a locally thickened (7-13-fold) cell wall	943:983	a locally thickened (7-13-fold) cell wall	943:983	Together with a locally thickened (7-13-fold) cell wall, increased content (up to 58%) of pectin and reduction in (up to 29%) hemicellulose were observed.
34339172	0	42	theme	nY<sub>2</sub>O<sub>3</sub>	38:64	arg1	Cytotoxicity					66:77	nY<sub>2</sub>O<sub>3</sub> Cytotoxicity	38:77	nY<sub>2</sub>O<sub>3</sub> Cytotoxicity	38:77	Cell Walls Are Remodeled to Alleviate nY<sub>2</sub>O<sub>3</sub> Cytotoxicity by Elaborate Regulation of <i>de Novo</i> Synthesis and Vesicular Transport.
34339172	2	43	theme	50	570:571	arg1	mg					573:574	mg	573:574	mg	573:574	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	6	44	theme	pectin	1272:1277	arg1	synthesis					1279:1287	pectin synthesis	1272:1287	pectin synthesis	1272:1287	Expression of genes for pectin synthesis and degradation was up- and down-regulated by 31-78% and 13-42%, respectively, and genes for xyloglucan and pectin modifications were up- and down-regulated by 82% and 81-92%, respectively.
34339172	2	45	theme	L<sup>-1</sup>	576:589	arg1	nY<sub>2</sub>O<sub>3</sub>					591:617	50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub>	570:617	50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm)	570:625	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	2	45	theme	L<sup>-1</sup>	576:589	arg1	nm					623:624	30 nm	620:624	30 nm	620:624	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	6	46	dep	xyloglucan	1382:1391	arg1	modifications					1404:1416	modifications	1404:1416	modifications	1404:1416	Expression of genes for pectin synthesis and degradation was up- and down-regulated by 31-78% and 13-42%, respectively, and genes for xyloglucan and pectin modifications were up- and down-regulated by 82% and 81-92%, respectively.
34339172	8	47	theme	nY<sub>2</sub>O<sub>3</sub>	1826:1852	arg1	stress					1854:1859	nY<sub>2</sub>O<sub>3</sub> stress	1826:1859	nY<sub>2</sub>O<sub>3</sub> stress	1826:1859	Our findings indicate that, although nY<sub>2</sub>O<sub>3</sub> generated toxicity on BY-2 cells, it is very likely that during the recovery process cell wall remodeling was initiated to gain resistance to nY<sub>2</sub>O<sub>3</sub> stress, demonstrating the plant's cellular regulatory machinery regarding repair and adaptation to nanoparticles like nY<sub>2</sub>O<sub>3</sub>.
34339172	3	48	theme	cell	908:911	arg1	walls					913:917	cell walls	908:917	cell walls	908:917	In addition to growth inhibition, morphological and compositional changes in cell walls occurred.
34339172	1	49	theme	earth	244:248	arg1	nanoparticles					250:262	the broadly used rare earth nanoparticles	222:262	the broadly used rare earth nanoparticles	222:262	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>), one of the broadly used rare earth nanoparticles, can interact with plants and possibly cause plant health and environmental impacts, but the plant defense response particularly at the nanoparticle-cell interface is largely unknown.
34339172	2	50	theme	mg	573:574	arg1	nY<sub>2</sub>O<sub>3</sub>					591:617	50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub>	570:617	50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm)	570:625	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	2	50	theme	mg	573:574	arg1	nm					623:624	30 nm	620:624	30 nm	620:624	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	7	51	dep	repair	1551:1556	arg1	the					1547:1549	the	1547:1549	the	1547:1549	Interestingly, vesicle trafficking seemed to be activated, enabling the repair and defense against nY<sub>2</sub>O<sub>3</sub> disturbance.
34339172	1	52	theme	Yttrium	156:162	arg1	oxide					164:168	Yttrium oxide	156:168	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>)	156:212	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>), one of the broadly used rare earth nanoparticles, can interact with plants and possibly cause plant health and environmental impacts, but the plant defense response particularly at the nanoparticle-cell interface is largely unknown.
34339172	3	53	theme	compositional	883:895	arg1	changes					897:903	morphological and compositional changes	865:903	morphological and compositional changes in cell walls	865:917	In addition to growth inhibition, morphological and compositional changes in cell walls occurred.
34339172	1	54	theme	nanoparticles	250:262	arg1	one					215:217	one	215:217	one	215:217	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>), one of the broadly used rare earth nanoparticles, can interact with plants and possibly cause plant health and environmental impacts, but the plant defense response particularly at the nanoparticle-cell interface is largely unknown.
34339172	1	54	theme	nanoparticles	250:262	arg1	nanoparticles					170:182	Yttrium oxide nanoparticles	156:182	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>)	156:212	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>), one of the broadly used rare earth nanoparticles, can interact with plants and possibly cause plant health and environmental impacts, but the plant defense response particularly at the nanoparticle-cell interface is largely unknown.
34339172	1	54	theme	nanoparticles	250:262	arg1	nanoparticles					250:262	the broadly used rare earth nanoparticles	222:262	the broadly used rare earth nanoparticles	222:262	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>), one of the broadly used rare earth nanoparticles, can interact with plants and possibly cause plant health and environmental impacts, but the plant defense response particularly at the nanoparticle-cell interface is largely unknown.
34339172	2	55	theme	tabacum</i>	512:522	arg1	L.					524:525	<i>Nicotiana tabacum</i> L.	499:525	<i>Nicotiana tabacum</i> L.	499:525	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	2	55	theme	tabacum</i>	512:522	arg1	cells					548:552	Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells	467:552	Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells	467:552	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	1	56	theme	rare	239:242	arg1	earth					244:248	the broadly used rare earth	222:248	the broadly used rare earth nanoparticles	222:262	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>), one of the broadly used rare earth nanoparticles, can interact with plants and possibly cause plant health and environmental impacts, but the plant defense response particularly at the nanoparticle-cell interface is largely unknown.
34339172	2	57	theme	cell	773:776	arg1	wall					778:781	the cell wall	769:781	the cell wall	769:781	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	1	58	theme	oxide	164:168	arg1	one					215:217	one	215:217	one	215:217	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>), one of the broadly used rare earth nanoparticles, can interact with plants and possibly cause plant health and environmental impacts, but the plant defense response particularly at the nanoparticle-cell interface is largely unknown.
34339172	1	58	theme	oxide	164:168	arg1	nanoparticles					170:182	Yttrium oxide nanoparticles	156:182	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>)	156:212	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>), one of the broadly used rare earth nanoparticles, can interact with plants and possibly cause plant health and environmental impacts, but the plant defense response particularly at the nanoparticle-cell interface is largely unknown.
34339172	1	58	theme	oxide	164:168	arg1	nanoparticles					250:262	the broadly used rare earth nanoparticles	222:262	the broadly used rare earth nanoparticles	222:262	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>), one of the broadly used rare earth nanoparticles, can interact with plants and possibly cause plant health and environmental impacts, but the plant defense response particularly at the nanoparticle-cell interface is largely unknown.
34339172	1	58	theme	oxide	164:168	arg1	nY<sub>2</sub>O<sub>3</sub>					185:211	nY<sub>2</sub>O<sub>3</sub>	185:211	nY<sub>2</sub>O<sub>3</sub>	185:211	Yttrium oxide nanoparticles (nY<sub>2</sub>O<sub>3</sub>), one of the broadly used rare earth nanoparticles, can interact with plants and possibly cause plant health and environmental impacts, but the plant defense response particularly at the nanoparticle-cell interface is largely unknown.
34339172	2	59	theme	<i>Nicotiana	499:510	arg1	L.					524:525	<i>Nicotiana tabacum</i> L.	499:525	<i>Nicotiana tabacum</i> L.	499:525	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	2	59	theme	<i>Nicotiana	499:510	arg1	cells					548:552	Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells	467:552	Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells	467:552	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	4	60	theme	increased	986:994	arg1	%					1013:1013	up to 58%	1005:1013	up to 58%	1005:1013	Together with a locally thickened (7-13-fold) cell wall, increased content (up to 58%) of pectin and reduction in (up to 29%) hemicellulose were observed.
34339172	4	60	theme	increased	986:994	arg1	content					996:1002	increased content	986:1002	increased content (up to 58%) of pectin	986:1024	Together with a locally thickened (7-13-fold) cell wall, increased content (up to 58%) of pectin and reduction in (up to 29%) hemicellulose were observed.
34339172	2	61	theme	suspension-cultured	528:546	arg1	L.					524:525	<i>Nicotiana tabacum</i> L.	499:525	<i>Nicotiana tabacum</i> L.	499:525	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
34339172	2	61	theme	suspension-cultured	528:546	arg1	cells					548:552	Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells	467:552	Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells	467:552	To elucidate this, Bright Yellow 2 (BY-2) tobacco (<i>Nicotiana tabacum</i> L.) suspension-cultured cells were exposed to 50 mg L<sup>-1</sup> nY<sub>2</sub>O<sub>3</sub> (30 nm) for 12 h. Although 42.2% of the nY<sub>2</sub>O<sub>3</sub> remained outside of protoplasts, nY<sub>2</sub>O<sub>3</sub> could still traverse the cell wall and was partially deposited inside the vacuole.
32045682	11	0	theme	administration	1931:1944	arg1	dose					1946:1949	the administration dose	1927:1949	the administration dose of 200 mg/kg	1927:1962	SYQP inhibited the growth of H22 tumor in mice with inhibitory rate of 39.9% at the administration dose of 200 mg/kg and increased the production of cytokines such as tumor necrosis factor-alpha (TNF-a) and interferon γ (IFN-γ).
32045682	3	1	theme	antitumor	814:822	arg1	effects					824:830	its antipyretic and antitumor effects	794:830	its antipyretic and antitumor effects in mice models	794:845	AIM OF THE STUDY The present study aimed to characterize the chemical properties of a purified polysaccharide extracted from the aerial part of Tetrastigma hemsleyanum (SYQP) and investigate its antipyretic and antitumor effects in mice models.
32045682	13	2	theme	host	2501:2504	arg1	function					2513:2520	host immune function	2501:2520	host immune function	2501:2520	CONCLUSION The anti-inflammatory and antitumor activities of SYQP might be related to its regulation of host immune function by controlling the secretion of cytokines.
32045682	12	3	theme	high	2191:2194	arg1	IL-10					2226:2230	IL-10	2226:2230	IL-10	2226:2230	Experimental results showed that the preventive administration of SYQP before lipopolysaccharide (LPS) reduced the high cytokine levels such as IL-6, IL-10 and IFN-γ, indicating that SYQP might act as a competitor with LPS to interact with toll like receptor 4 (TLR4), which further regulated the secretion of cytokines.
32045682	12	3	theme	high	2191:2194	arg1	levels					2205:2210	the high cytokine levels	2187:2210	the high cytokine levels such as IL-6, IL-10 and IFN-γ	2187:2240	Experimental results showed that the preventive administration of SYQP before lipopolysaccharide (LPS) reduced the high cytokine levels such as IL-6, IL-10 and IFN-γ, indicating that SYQP might act as a competitor with LPS to interact with toll like receptor 4 (TLR4), which further regulated the secretion of cytokines.
32045682	12	3	theme	high	2191:2194	arg1	IL-6					2220:2223	IL-6	2220:2223	IL-6	2220:2223	Experimental results showed that the preventive administration of SYQP before lipopolysaccharide (LPS) reduced the high cytokine levels such as IL-6, IL-10 and IFN-γ, indicating that SYQP might act as a competitor with LPS to interact with toll like receptor 4 (TLR4), which further regulated the secretion of cytokines.
32045682	12	3	theme	high	2191:2194	arg1	IFN-γ					2236:2240	IFN-γ	2236:2240	IFN-γ	2236:2240	Experimental results showed that the preventive administration of SYQP before lipopolysaccharide (LPS) reduced the high cytokine levels such as IL-6, IL-10 and IFN-γ, indicating that SYQP might act as a competitor with LPS to interact with toll like receptor 4 (TLR4), which further regulated the secretion of cytokines.
32045682	6	4	theme	hyperthermia	1217:1228	arg1	test					1230:1233	Brewer's yeast induced hyperthermia test	1194:1233	Brewer's yeast induced hyperthermia test	1194:1233	Antipyretic effect of SYQP was examined using Brewer's yeast induced hyperthermia test.
32045682	1	5	theme	fever	272:276	arg1	treatments					239:248	the treatments	235:248	the treatments of inflammation, high fever, hepatitis and cancer	235:298	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	9	6	theme	molar	1529:1533	arg1	ratio					1535:1539	a molar ratio	1527:1539	a molar ratio of 11.3:7.1:2.5:1.0:0.9:0.5	1527:1567	RESULTS SYQP was composed of galacturonic acid (GalA), glucose (Glc), mannose (Man), arabinose (Ara), galactose (Gal), and rhamnose (Rha) with a molar ratio of 11.3:7.1:2.5:1.0:0.9:0.5 and it had an average molecular weight of 66.2 kDa.
32045682	6	7	theme	yeast	1203:1207	arg1	test					1230:1233	Brewer's yeast induced hyperthermia test	1194:1233	Brewer's yeast induced hyperthermia test	1194:1233	Antipyretic effect of SYQP was examined using Brewer's yeast induced hyperthermia test.
32045682	5	8	theme	molecular	1043:1051	arg1	weight					1053:1058	molecular weight	1043:1058	molecular weight	1043:1058	Homogeneity, molecular weight, monosaccharide composition, and FTIR analysis were performed to characterize the SYQP.
32045682	11	9	theme	%	1922:1922	arg1	rate					1910:1913	inhibitory rate	1899:1913	inhibitory rate of 39.9% at the administration dose of 200 mg/kg	1899:1962	SYQP inhibited the growth of H22 tumor in mice with inhibitory rate of 39.9% at the administration dose of 200 mg/kg and increased the production of cytokines such as tumor necrosis factor-alpha (TNF-a) and interferon γ (IFN-γ).
32045682	10	10	theme	SYQP	1648:1651	arg1	administration					1630:1643	The oral administration	1621:1643	The oral administration of SYQP at 200 and 400 mg/kg	1621:1672	The oral administration of SYQP at 200 and 400 mg/kg could markedly suppress the hyperthermia of mice induced by Brewer's yeast and decrease the production of cytokines especially prostaglandin E2 (PGE2) in the serum of mice.
32045682	9	11	theme	galacturonic	1413:1424	arg1	GalA					1432:1435	GalA	1432:1435	GalA	1432:1435	RESULTS SYQP was composed of galacturonic acid (GalA), glucose (Glc), mannose (Man), arabinose (Ara), galactose (Gal), and rhamnose (Rha) with a molar ratio of 11.3:7.1:2.5:1.0:0.9:0.5 and it had an average molecular weight of 66.2 kDa.
32045682	9	11	theme	galacturonic	1413:1424	arg1	acid					1426:1429	galacturonic acid	1413:1429	galacturonic acid (GalA)	1413:1436	RESULTS SYQP was composed of galacturonic acid (GalA), glucose (Glc), mannose (Man), arabinose (Ara), galactose (Gal), and rhamnose (Rha) with a molar ratio of 11.3:7.1:2.5:1.0:0.9:0.5 and it had an average molecular weight of 66.2 kDa.
32045682	13	12	theme	SYQP	2458:2461	arg1	related					2472:2478	related	2472:2478	related	2472:2478	CONCLUSION The anti-inflammatory and antitumor activities of SYQP might be related to its regulation of host immune function by controlling the secretion of cytokines.
32045682	13	12	theme	SYQP	2458:2461	arg1	activities					2444:2453	The anti-inflammatory and antitumor activities	2408:2453	The anti-inflammatory and antitumor activities of SYQP	2408:2461	CONCLUSION The anti-inflammatory and antitumor activities of SYQP might be related to its regulation of host immune function by controlling the secretion of cytokines.
32045682	0	13	from	parts	75:79	arg1	effects					26:32	Antipyretic and antitumor effects	0:32	Antipyretic and antitumor effects of a purified polysaccharide from aerial parts of Tetrastigma hemsleyanum.	0:107	Antipyretic and antitumor effects of a purified polysaccharide from aerial parts of Tetrastigma hemsleyanum.
32045682	10	14	theme	mice	1841:1844	arg1	serum					1832:1836	the serum	1828:1836	the serum of mice	1828:1844	The oral administration of SYQP at 200 and 400 mg/kg could markedly suppress the hyperthermia of mice induced by Brewer's yeast and decrease the production of cytokines especially prostaglandin E2 (PGE2) in the serum of mice.
32045682	9	15	theme	average	1583:1589	arg1	weight					1601:1606	an average molecular weight	1580:1606	an average molecular weight of 66.2 kDa	1580:1618	RESULTS SYQP was composed of galacturonic acid (GalA), glucose (Glc), mannose (Man), arabinose (Ara), galactose (Gal), and rhamnose (Rha) with a molar ratio of 11.3:7.1:2.5:1.0:0.9:0.5 and it had an average molecular weight of 66.2 kDa.
32045682	12	16	with	competitor	2279:2288	arg1	LPS					2295:2297	LPS	2295:2297	LPS	2295:2297	Experimental results showed that the preventive administration of SYQP before lipopolysaccharide (LPS) reduced the high cytokine levels such as IL-6, IL-10 and IFN-γ, indicating that SYQP might act as a competitor with LPS to interact with toll like receptor 4 (TLR4), which further regulated the secretion of cytokines.
32045682	12	17	theme	preventive	2113:2122	arg1	administration					2124:2137	the preventive administration	2109:2137	the preventive administration of SYQP before lipopolysaccharide (LPS)	2109:2177	Experimental results showed that the preventive administration of SYQP before lipopolysaccharide (LPS) reduced the high cytokine levels such as IL-6, IL-10 and IFN-γ, indicating that SYQP might act as a competitor with LPS to interact with toll like receptor 4 (TLR4), which further regulated the secretion of cytokines.
32045682	1	18	used	used	207:210	arg2	medicine					222:229	a folk medicine	215:229	a folk medicine	215:229	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	1	18	used	used	207:210	arg2	RELEVANCE					130:138	ETHNOPHARMACOLOGICAL RELEVANCE	109:138	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing)	109:188	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	1	18	used	used	207:210	arg2	function					328:335	the immune function	317:335	the immune function of the patient	317:350	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	11	19	from	rate	1910:1913	arg1	dose					1946:1949	the administration dose	1927:1949	the administration dose of 200 mg/kg	1927:1962	SYQP inhibited the growth of H22 tumor in mice with inhibitory rate of 39.9% at the administration dose of 200 mg/kg and increased the production of cytokines such as tumor necrosis factor-alpha (TNF-a) and interferon γ (IFN-γ).
32045682	3	20	theme	hemsleyanum	759:769	arg1	part					739:742	the aerial part	728:742	the aerial part of Tetrastigma hemsleyanum (SYQP)	728:776	AIM OF THE STUDY The present study aimed to characterize the chemical properties of a purified polysaccharide extracted from the aerial part of Tetrastigma hemsleyanum (SYQP) and investigate its antipyretic and antitumor effects in mice models.
32045682	9	21	theme	66.2 kDa	1611:1618	arg1	weight					1601:1606	an average molecular weight	1580:1606	an average molecular weight of 66.2 kDa	1580:1618	RESULTS SYQP was composed of galacturonic acid (GalA), glucose (Glc), mannose (Man), arabinose (Ara), galactose (Gal), and rhamnose (Rha) with a molar ratio of 11.3:7.1:2.5:1.0:0.9:0.5 and it had an average molecular weight of 66.2 kDa.
32045682	6	22	theme	Antipyretic	1148:1158	arg1	effect					1160:1165	Antipyretic effect	1148:1165	Antipyretic effect of SYQP	1148:1173	Antipyretic effect of SYQP was examined using Brewer's yeast induced hyperthermia test.
32045682	13	23	theme	anti-inflammatory	2412:2428	arg1	related					2472:2478	related	2472:2478	related	2472:2478	CONCLUSION The anti-inflammatory and antitumor activities of SYQP might be related to its regulation of host immune function by controlling the secretion of cytokines.
32045682	13	23	theme	anti-inflammatory	2412:2428	arg1	activities					2444:2453	The anti-inflammatory and antitumor activities	2408:2453	The anti-inflammatory and antitumor activities of SYQP	2408:2461	CONCLUSION The anti-inflammatory and antitumor activities of SYQP might be related to its regulation of host immune function by controlling the secretion of cytokines.
32045682	3	24	theme	polysaccharide	698:711	arg1	properties					673:682	the chemical properties	660:682	the chemical properties of a purified polysaccharide extracted from the aerial part of Tetrastigma hemsleyanum (SYQP)	660:776	AIM OF THE STUDY The present study aimed to characterize the chemical properties of a purified polysaccharide extracted from the aerial part of Tetrastigma hemsleyanum (SYQP) and investigate its antipyretic and antitumor effects in mice models.
32045682	1	25	theme	high	267:270	arg1	fever					272:276	high fever	267:276	high fever	267:276	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	5	26	theme	monosaccharide	1061:1074	arg1	composition					1076:1086	monosaccharide composition	1061:1086	monosaccharide composition	1061:1086	Homogeneity, molecular weight, monosaccharide composition, and FTIR analysis were performed to characterize the SYQP.
32045682	4	27	theme	aerial	915:920	arg1	parts					922:926	the aerial parts	911:926	the aerial parts of Tetrastigma hemsleyanum	911:953	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of Tetrastigma hemsleyanum were extracted and fractionated by DEAE and gel permeation chromatography.
32045682	13	28	theme	antitumor	2434:2442	arg1	related					2472:2478	related	2472:2478	related	2472:2478	CONCLUSION The anti-inflammatory and antitumor activities of SYQP might be related to its regulation of host immune function by controlling the secretion of cytokines.
32045682	13	28	theme	antitumor	2434:2442	arg1	activities					2444:2453	The anti-inflammatory and antitumor activities	2408:2453	The anti-inflammatory and antitumor activities of SYQP	2408:2461	CONCLUSION The anti-inflammatory and antitumor activities of SYQP might be related to its regulation of host immune function by controlling the secretion of cytokines.
32045682	3	29	theme	aerial	732:737	arg1	part					739:742	the aerial part	728:742	the aerial part of Tetrastigma hemsleyanum (SYQP)	728:776	AIM OF THE STUDY The present study aimed to characterize the chemical properties of a purified polysaccharide extracted from the aerial part of Tetrastigma hemsleyanum (SYQP) and investigate its antipyretic and antitumor effects in mice models.
32045682	0	30	theme	Tetrastigma	84:94	arg1	hemsleyanum					96:106	Tetrastigma hemsleyanum	84:106	Tetrastigma hemsleyanum	84:106	Antipyretic and antitumor effects of a purified polysaccharide from aerial parts of Tetrastigma hemsleyanum.
32045682	4	31	theme	crude	884:888	arg1	polysaccharides					890:904	Water-soluble crude polysaccharides	870:904	Water-soluble crude polysaccharides	870:904	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of Tetrastigma hemsleyanum were extracted and fractionated by DEAE and gel permeation chromatography.
32045682	1	32	dep	RELEVANCE	130:138	arg1	Tetrastigma					140:150	Tetrastigma	140:150	Tetrastigma	140:150	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	3	33	from	effects	824:830	arg1	models					840:845	mice models	835:845	mice models	835:845	AIM OF THE STUDY The present study aimed to characterize the chemical properties of a purified polysaccharide extracted from the aerial part of Tetrastigma hemsleyanum (SYQP) and investigate its antipyretic and antitumor effects in mice models.
32045682	13	34	dep	CONCLUSION	2397:2406	arg1	related					2472:2478	related	2472:2478	related	2472:2478	CONCLUSION The anti-inflammatory and antitumor activities of SYQP might be related to its regulation of host immune function by controlling the secretion of cytokines.
32045682	13	34	dep	CONCLUSION	2397:2406	arg1	activities					2444:2453	The anti-inflammatory and antitumor activities	2408:2453	The anti-inflammatory and antitumor activities of SYQP	2408:2461	CONCLUSION The anti-inflammatory and antitumor activities of SYQP might be related to its regulation of host immune function by controlling the secretion of cytokines.
32045682	0	35	theme	Antipyretic	0:10	arg1	effects					26:32	Antipyretic and antitumor effects	0:32	Antipyretic and antitumor effects of a purified polysaccharide from aerial parts of Tetrastigma hemsleyanum.	0:107	Antipyretic and antitumor effects of a purified polysaccharide from aerial parts of Tetrastigma hemsleyanum.
32045682	13	36	theme	cytokines	2554:2562	arg1	secretion					2541:2549	the secretion	2537:2549	the secretion of cytokines	2537:2562	CONCLUSION The anti-inflammatory and antitumor activities of SYQP might be related to its regulation of host immune function by controlling the secretion of cytokines.
32045682	7	37	theme	tumor	1280:1284	arg1	mice					1294:1297	H22 tumor bearing mice	1276:1297	H22 tumor bearing mice	1276:1297	Antitumor effect was investigated using H22 tumor bearing mice.
32045682	10	38	dep	cytokines	1780:1788	arg1	PGE2					1819:1822	PGE2	1819:1822	PGE2	1819:1822	The oral administration of SYQP at 200 and 400 mg/kg could markedly suppress the hyperthermia of mice induced by Brewer's yeast and decrease the production of cytokines especially prostaglandin E2 (PGE2) in the serum of mice.
32045682	10	38	dep	cytokines	1780:1788	arg1	E2					1815:1816	prostaglandin E2	1801:1816	cytokines especially prostaglandin E2 (PGE2)	1780:1823	The oral administration of SYQP at 200 and 400 mg/kg could markedly suppress the hyperthermia of mice induced by Brewer's yeast and decrease the production of cytokines especially prostaglandin E2 (PGE2) in the serum of mice.
32045682	0	39	theme	antitumor	16:24	arg1	effects					26:32	Antipyretic and antitumor effects	0:32	Antipyretic and antitumor effects of a purified polysaccharide from aerial parts of Tetrastigma hemsleyanum.	0:107	Antipyretic and antitumor effects of a purified polysaccharide from aerial parts of Tetrastigma hemsleyanum.
32045682	1	40	theme	ETHNOPHARMACOLOGICAL	109:128	arg1	function					328:335	the immune function	317:335	the immune function of the patient	317:350	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	1	40	theme	ETHNOPHARMACOLOGICAL	109:128	arg1	RELEVANCE					130:138	ETHNOPHARMACOLOGICAL RELEVANCE	109:138	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing)	109:188	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	1	40	theme	ETHNOPHARMACOLOGICAL	109:128	arg1	medicine					222:229	a folk medicine	215:229	a folk medicine	215:229	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	3	41	theme	mice	835:838	arg1	models					840:845	mice models	835:845	mice models	835:845	AIM OF THE STUDY The present study aimed to characterize the chemical properties of a purified polysaccharide extracted from the aerial part of Tetrastigma hemsleyanum (SYQP) and investigate its antipyretic and antitumor effects in mice models.
32045682	11	42	with	mice	1889:1892	arg1	rate					1910:1913	inhibitory rate	1899:1913	inhibitory rate of 39.9% at the administration dose of 200 mg/kg	1899:1962	SYQP inhibited the growth of H22 tumor in mice with inhibitory rate of 39.9% at the administration dose of 200 mg/kg and increased the production of cytokines such as tumor necrosis factor-alpha (TNF-a) and interferon γ (IFN-γ).
32045682	11	43	theme	tumor	2014:2018	arg1	TNF-a					2043:2047	TNF-a	2043:2047	TNF-a	2043:2047	SYQP inhibited the growth of H22 tumor in mice with inhibitory rate of 39.9% at the administration dose of 200 mg/kg and increased the production of cytokines such as tumor necrosis factor-alpha (TNF-a) and interferon γ (IFN-γ).
32045682	11	43	theme	tumor	2014:2018	arg1	factor-alpha					2029:2040	tumor necrosis factor-alpha	2014:2040	tumor necrosis factor-alpha (TNF-a)	2014:2048	SYQP inhibited the growth of H22 tumor in mice with inhibitory rate of 39.9% at the administration dose of 200 mg/kg and increased the production of cytokines such as tumor necrosis factor-alpha (TNF-a) and interferon γ (IFN-γ).
32045682	8	44	theme	biological	1353:1362	arg1	activities					1364:1373	the biological activities	1349:1373	the biological activities of SYQP	1349:1381	The serum cytokines were determined to evaluated the biological activities of SYQP.
32045682	2	45	theme	Vitaceae	381:388	arg1	family					371:376	the family	367:376	the family of Vitaceae	367:388	It belongs to the family of Vitaceae, and is mainly distributed in southeast China (Yunnan province) and can be found in India (Andaman Islands), Myanmar, Thailand, Vietnam, Malaysia and Indonesia in the valleys with 1100-1300 m above the sea level.
32045682	5	46	theme	FTIR	1093:1096	arg1	analysis					1098:1105	FTIR analysis	1093:1105	FTIR analysis	1093:1105	Homogeneity, molecular weight, monosaccharide composition, and FTIR analysis were performed to characterize the SYQP.
32045682	4	47	theme	hemsleyanum	943:953	arg1	parts					922:926	the aerial parts	911:926	the aerial parts of Tetrastigma hemsleyanum	911:953	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of Tetrastigma hemsleyanum were extracted and fractionated by DEAE and gel permeation chromatography.
32045682	2	48	with	valleys	557:563	arg1	1100-1300 m					570:580	1100-1300 m	570:580	1100-1300 m	570:580	It belongs to the family of Vitaceae, and is mainly distributed in southeast China (Yunnan province) and can be found in India (Andaman Islands), Myanmar, Thailand, Vietnam, Malaysia and Indonesia in the valleys with 1100-1300 m above the sea level.
32045682	11	49	theme	200 mg/kg	1954:1962	arg1	dose					1946:1949	the administration dose	1927:1949	the administration dose of 200 mg/kg	1927:1962	SYQP inhibited the growth of H22 tumor in mice with inhibitory rate of 39.9% at the administration dose of 200 mg/kg and increased the production of cytokines such as tumor necrosis factor-alpha (TNF-a) and interferon γ (IFN-γ).
32045682	0	50	theme	purified	39:46	arg1	polysaccharide					48:61	a purified polysaccharide	37:61	a purified polysaccharide	37:61	Antipyretic and antitumor effects of a purified polysaccharide from aerial parts of Tetrastigma hemsleyanum.
32045682	7	51	theme	Antitumor	1236:1244	arg1	effect					1246:1251	Antitumor effect	1236:1251	Antitumor effect	1236:1251	Antitumor effect was investigated using H22 tumor bearing mice.
32045682	11	52	from	growth	1866:1871	arg1	mice					1889:1892	mice	1889:1892	mice with inhibitory rate of 39.9% at the administration dose of 200 mg/kg	1889:1962	SYQP inhibited the growth of H22 tumor in mice with inhibitory rate of 39.9% at the administration dose of 200 mg/kg and increased the production of cytokines such as tumor necrosis factor-alpha (TNF-a) and interferon γ (IFN-γ).
32045682	11	53	theme	tumor	1880:1884	arg1	growth					1866:1871	the growth	1862:1871	the growth of H22 tumor in mice with inhibitory rate of 39.9% at the administration dose of 200 mg/kg	1862:1962	SYQP inhibited the growth of H22 tumor in mice with inhibitory rate of 39.9% at the administration dose of 200 mg/kg and increased the production of cytokines such as tumor necrosis factor-alpha (TNF-a) and interferon γ (IFN-γ).
32045682	12	54	theme	cytokine	2196:2203	arg1	IL-10					2226:2230	IL-10	2226:2230	IL-10	2226:2230	Experimental results showed that the preventive administration of SYQP before lipopolysaccharide (LPS) reduced the high cytokine levels such as IL-6, IL-10 and IFN-γ, indicating that SYQP might act as a competitor with LPS to interact with toll like receptor 4 (TLR4), which further regulated the secretion of cytokines.
32045682	12	54	theme	cytokine	2196:2203	arg1	levels					2205:2210	the high cytokine levels	2187:2210	the high cytokine levels such as IL-6, IL-10 and IFN-γ	2187:2240	Experimental results showed that the preventive administration of SYQP before lipopolysaccharide (LPS) reduced the high cytokine levels such as IL-6, IL-10 and IFN-γ, indicating that SYQP might act as a competitor with LPS to interact with toll like receptor 4 (TLR4), which further regulated the secretion of cytokines.
32045682	12	54	theme	cytokine	2196:2203	arg1	IL-6					2220:2223	IL-6	2220:2223	IL-6	2220:2223	Experimental results showed that the preventive administration of SYQP before lipopolysaccharide (LPS) reduced the high cytokine levels such as IL-6, IL-10 and IFN-γ, indicating that SYQP might act as a competitor with LPS to interact with toll like receptor 4 (TLR4), which further regulated the secretion of cytokines.
32045682	12	54	theme	cytokine	2196:2203	arg1	IFN-γ					2236:2240	IFN-γ	2236:2240	IFN-γ	2236:2240	Experimental results showed that the preventive administration of SYQP before lipopolysaccharide (LPS) reduced the high cytokine levels such as IL-6, IL-10 and IFN-γ, indicating that SYQP might act as a competitor with LPS to interact with toll like receptor 4 (TLR4), which further regulated the secretion of cytokines.
32045682	4	55	dep	MATERIALS	848:856	arg1	polysaccharides					890:904	Water-soluble crude polysaccharides	870:904	Water-soluble crude polysaccharides	870:904	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of Tetrastigma hemsleyanum were extracted and fractionated by DEAE and gel permeation chromatography.
32045682	4	56	theme	DEAE	990:993	arg1	chromatography					1014:1027	DEAE and gel permeation chromatography	990:1027	chromatography	1014:1027	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of Tetrastigma hemsleyanum were extracted and fractionated by DEAE and gel permeation chromatography.
32045682	3	57	theme	STUDY	614:618	arg1	AIM					603:605	AIM	603:605	AIM OF THE STUDY	603:618	AIM OF THE STUDY The present study aimed to characterize the chemical properties of a purified polysaccharide extracted from the aerial part of Tetrastigma hemsleyanum (SYQP) and investigate its antipyretic and antitumor effects in mice models.
32045682	3	58	theme	present	624:630	arg1	study					632:636	The present study	620:636	The present study	620:636	AIM OF THE STUDY The present study aimed to characterize the chemical properties of a purified polysaccharide extracted from the aerial part of Tetrastigma hemsleyanum (SYQP) and investigate its antipyretic and antitumor effects in mice models.
32045682	1	59	theme	inflammation	253:264	arg1	treatments					239:248	the treatments	235:248	the treatments of inflammation, high fever, hepatitis and cancer	235:298	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	4	60	theme	gel	999:1001	arg1	chromatography					1014:1027	DEAE and gel permeation chromatography	990:1027	chromatography	1014:1027	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of Tetrastigma hemsleyanum were extracted and fractionated by DEAE and gel permeation chromatography.
32045682	10	61	theme	oral	1625:1628	arg1	administration					1630:1643	The oral administration	1621:1643	The oral administration of SYQP at 200 and 400 mg/kg	1621:1672	The oral administration of SYQP at 200 and 400 mg/kg could markedly suppress the hyperthermia of mice induced by Brewer's yeast and decrease the production of cytokines especially prostaglandin E2 (PGE2) in the serum of mice.
32045682	13	62	theme	immune	2506:2511	arg1	function					2513:2520	host immune function	2501:2520	host immune function	2501:2520	CONCLUSION The anti-inflammatory and antitumor activities of SYQP might be related to its regulation of host immune function by controlling the secretion of cytokines.
32045682	9	63	theme	RESULTS	1384:1390	arg1	SYQP					1392:1395	RESULTS SYQP	1384:1395	RESULTS SYQP	1384:1395	RESULTS SYQP was composed of galacturonic acid (GalA), glucose (Glc), mannose (Man), arabinose (Ara), galactose (Gal), and rhamnose (Rha) with a molar ratio of 11.3:7.1:2.5:1.0:0.9:0.5 and it had an average molecular weight of 66.2 kDa.
32045682	10	64	theme	prostaglandin	1801:1813	arg1	PGE2					1819:1822	PGE2	1819:1822	PGE2	1819:1822	The oral administration of SYQP at 200 and 400 mg/kg could markedly suppress the hyperthermia of mice induced by Brewer's yeast and decrease the production of cytokines especially prostaglandin E2 (PGE2) in the serum of mice.
32045682	10	64	theme	prostaglandin	1801:1813	arg1	E2					1815:1816	prostaglandin E2	1801:1816	cytokines especially prostaglandin E2 (PGE2)	1780:1823	The oral administration of SYQP at 200 and 400 mg/kg could markedly suppress the hyperthermia of mice induced by Brewer's yeast and decrease the production of cytokines especially prostaglandin E2 (PGE2) in the serum of mice.
32045682	6	65	theme	induced	1209:1215	arg1	test					1230:1233	Brewer's yeast induced hyperthermia test	1194:1233	Brewer's yeast induced hyperthermia test	1194:1233	Antipyretic effect of SYQP was examined using Brewer's yeast induced hyperthermia test.
32045682	10	66	theme	cytokines	1780:1788	arg1	production					1766:1775	the production	1762:1775	the production of cytokines especially prostaglandin E2 (PGE2) in the serum of mice	1762:1844	The oral administration of SYQP at 200 and 400 mg/kg could markedly suppress the hyperthermia of mice induced by Brewer's yeast and decrease the production of cytokines especially prostaglandin E2 (PGE2) in the serum of mice.
32045682	11	67	theme	H22	1876:1878	arg1	tumor					1880:1884	H22 tumor	1876:1884	H22 tumor	1876:1884	SYQP inhibited the growth of H22 tumor in mice with inhibitory rate of 39.9% at the administration dose of 200 mg/kg and increased the production of cytokines such as tumor necrosis factor-alpha (TNF-a) and interferon γ (IFN-γ).
32045682	9	68	theme	11.3:7.1:2.5:1.0:0.9:0.5	1544:1567	arg1	ratio					1535:1539	a molar ratio	1527:1539	a molar ratio of 11.3:7.1:2.5:1.0:0.9:0.5	1527:1567	RESULTS SYQP was composed of galacturonic acid (GalA), glucose (Glc), mannose (Man), arabinose (Ara), galactose (Gal), and rhamnose (Rha) with a molar ratio of 11.3:7.1:2.5:1.0:0.9:0.5 and it had an average molecular weight of 66.2 kDa.
32045682	3	69	theme	chemical	664:671	arg1	properties					673:682	the chemical properties	660:682	the chemical properties of a purified polysaccharide extracted from the aerial part of Tetrastigma hemsleyanum (SYQP)	660:776	AIM OF THE STUDY The present study aimed to characterize the chemical properties of a purified polysaccharide extracted from the aerial part of Tetrastigma hemsleyanum (SYQP) and investigate its antipyretic and antitumor effects in mice models.
32045682	10	70	from	production	1766:1775	arg1	serum					1832:1836	the serum	1828:1836	the serum of mice	1828:1844	The oral administration of SYQP at 200 and 400 mg/kg could markedly suppress the hyperthermia of mice induced by Brewer's yeast and decrease the production of cytokines especially prostaglandin E2 (PGE2) in the serum of mice.
32045682	11	71	theme	inhibitory	1899:1908	arg1	rate					1910:1913	inhibitory rate	1899:1913	inhibitory rate of 39.9% at the administration dose of 200 mg/kg	1899:1962	SYQP inhibited the growth of H22 tumor in mice with inhibitory rate of 39.9% at the administration dose of 200 mg/kg and increased the production of cytokines such as tumor necrosis factor-alpha (TNF-a) and interferon γ (IFN-γ).
32045682	1	72	theme	folk	217:220	arg1	function					328:335	the immune function	317:335	the immune function of the patient	317:350	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	1	72	theme	folk	217:220	arg1	RELEVANCE					130:138	ETHNOPHARMACOLOGICAL RELEVANCE	109:138	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing)	109:188	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	1	72	theme	folk	217:220	arg1	medicine					222:229	a folk medicine	215:229	a folk medicine	215:229	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	3	73	dep	aimed	638:642	arg1	AIM					603:605	AIM	603:605	AIM OF THE STUDY	603:618	AIM OF THE STUDY The present study aimed to characterize the chemical properties of a purified polysaccharide extracted from the aerial part of Tetrastigma hemsleyanum (SYQP) and investigate its antipyretic and antitumor effects in mice models.
32045682	3	74	theme	purified	689:696	arg1	polysaccharide					698:711	a purified polysaccharide	687:711	a purified polysaccharide extracted from the aerial part of Tetrastigma hemsleyanum (SYQP)	687:776	AIM OF THE STUDY The present study aimed to characterize the chemical properties of a purified polysaccharide extracted from the aerial part of Tetrastigma hemsleyanum (SYQP) and investigate its antipyretic and antitumor effects in mice models.
32045682	9	75	theme	molecular	1591:1599	arg1	weight					1601:1606	an average molecular weight	1580:1606	an average molecular weight of 66.2 kDa	1580:1618	RESULTS SYQP was composed of galacturonic acid (GalA), glucose (Glc), mannose (Man), arabinose (Ara), galactose (Gal), and rhamnose (Rha) with a molar ratio of 11.3:7.1:2.5:1.0:0.9:0.5 and it had an average molecular weight of 66.2 kDa.
32045682	6	76	theme	SYQP	1170:1173	arg1	effect					1160:1165	Antipyretic effect	1148:1165	Antipyretic effect of SYQP	1148:1173	Antipyretic effect of SYQP was examined using Brewer's yeast induced hyperthermia test.
32045682	11	77	from	dose	1946:1949	arg1	rate					1910:1913	inhibitory rate	1899:1913	inhibitory rate of 39.9% at the administration dose of 200 mg/kg	1899:1962	SYQP inhibited the growth of H22 tumor in mice with inhibitory rate of 39.9% at the administration dose of 200 mg/kg and increased the production of cytokines such as tumor necrosis factor-alpha (TNF-a) and interferon γ (IFN-γ).
32045682	11	77	from	dose	1946:1949	arg1	%					1922:1922	39.9%	1918:1922	39.9% at the administration dose of 200 mg/kg	1918:1962	SYQP inhibited the growth of H22 tumor in mice with inhibitory rate of 39.9% at the administration dose of 200 mg/kg and increased the production of cytokines such as tumor necrosis factor-alpha (TNF-a) and interferon γ (IFN-γ).
32045682	12	78	theme	SYQP	2142:2145	arg1	administration					2124:2137	the preventive administration	2109:2137	the preventive administration of SYQP before lipopolysaccharide (LPS)	2109:2177	Experimental results showed that the preventive administration of SYQP before lipopolysaccharide (LPS) reduced the high cytokine levels such as IL-6, IL-10 and IFN-γ, indicating that SYQP might act as a competitor with LPS to interact with toll like receptor 4 (TLR4), which further regulated the secretion of cytokines.
32045682	12	79	theme	cytokines	2386:2394	arg1	secretion					2373:2381	the secretion	2369:2381	the secretion of cytokines	2369:2394	Experimental results showed that the preventive administration of SYQP before lipopolysaccharide (LPS) reduced the high cytokine levels such as IL-6, IL-10 and IFN-γ, indicating that SYQP might act as a competitor with LPS to interact with toll like receptor 4 (TLR4), which further regulated the secretion of cytokines.
32045682	0	80	theme	aerial	68:73	arg1	parts					75:79	aerial parts	68:79	aerial parts of Tetrastigma hemsleyanum	68:106	Antipyretic and antitumor effects of a purified polysaccharide from aerial parts of Tetrastigma hemsleyanum.
32045682	1	81	theme	immune	321:326	arg1	function					328:335	the immune function	317:335	the immune function of the patient	317:350	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	1	81	theme	immune	321:326	arg1	RELEVANCE					130:138	ETHNOPHARMACOLOGICAL RELEVANCE	109:138	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing)	109:188	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	1	81	theme	immune	321:326	arg1	medicine					222:229	a folk medicine	215:229	a folk medicine	215:229	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	3	82	theme	antipyretic	798:808	arg1	effects					824:830	its antipyretic and antitumor effects	794:830	its antipyretic and antitumor effects in mice models	794:845	AIM OF THE STUDY The present study aimed to characterize the chemical properties of a purified polysaccharide extracted from the aerial part of Tetrastigma hemsleyanum (SYQP) and investigate its antipyretic and antitumor effects in mice models.
32045682	3	83	theme	Tetrastigma	747:757	arg1	SYQP					772:775	SYQP	772:775	SYQP	772:775	AIM OF THE STUDY The present study aimed to characterize the chemical properties of a purified polysaccharide extracted from the aerial part of Tetrastigma hemsleyanum (SYQP) and investigate its antipyretic and antitumor effects in mice models.
32045682	3	83	theme	Tetrastigma	747:757	arg1	hemsleyanum					759:769	Tetrastigma hemsleyanum	747:769	Tetrastigma hemsleyanum (SYQP)	747:776	AIM OF THE STUDY The present study aimed to characterize the chemical properties of a purified polysaccharide extracted from the aerial part of Tetrastigma hemsleyanum (SYQP) and investigate its antipyretic and antitumor effects in mice models.
32045682	0	84	theme	hemsleyanum	96:106	arg1	parts					75:79	aerial parts	68:79	aerial parts of Tetrastigma hemsleyanum	68:106	Antipyretic and antitumor effects of a purified polysaccharide from aerial parts of Tetrastigma hemsleyanum.
32045682	1	85	dep	Diels	164:168	arg1	Gilg					173:176	Diels et Gilg	164:176	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing)	109:188	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	1	86	theme	patient	344:350	arg1	function					328:335	the immune function	317:335	the immune function of the patient	317:350	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	1	86	theme	patient	344:350	arg1	RELEVANCE					130:138	ETHNOPHARMACOLOGICAL RELEVANCE	109:138	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing)	109:188	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	1	86	theme	patient	344:350	arg1	medicine					222:229	a folk medicine	215:229	a folk medicine	215:229	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	10	87	from	200	1656:1658	arg1	administration					1630:1643	The oral administration	1621:1643	The oral administration of SYQP at 200 and 400 mg/kg	1621:1672	The oral administration of SYQP at 200 and 400 mg/kg could markedly suppress the hyperthermia of mice induced by Brewer's yeast and decrease the production of cytokines especially prostaglandin E2 (PGE2) in the serum of mice.
32045682	7	88	theme	bearing	1286:1292	arg1	mice					1294:1297	H22 tumor bearing mice	1276:1297	H22 tumor bearing mice	1276:1297	Antitumor effect was investigated using H22 tumor bearing mice.
32045682	4	89	theme	Water-soluble	870:882	arg1	polysaccharides					890:904	Water-soluble crude polysaccharides	870:904	Water-soluble crude polysaccharides	870:904	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of Tetrastigma hemsleyanum were extracted and fractionated by DEAE and gel permeation chromatography.
32045682	12	90	theme	Experimental	2076:2087	arg1	results					2089:2095	Experimental results	2076:2095	Experimental results	2076:2095	Experimental results showed that the preventive administration of SYQP before lipopolysaccharide (LPS) reduced the high cytokine levels such as IL-6, IL-10 and IFN-γ, indicating that SYQP might act as a competitor with LPS to interact with toll like receptor 4 (TLR4), which further regulated the secretion of cytokines.
32045682	1	91	dep	Tetrastigma	140:150	arg1	hemsleyanum					152:162	Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing)	140:188	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing)	109:188	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	1	91	dep	Tetrastigma	140:150	arg1	Diels					164:168	Diels	164:168	Diels	164:168	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	1	91	dep	Tetrastigma	140:150	arg1	Sanyeqing					179:187	Sanyeqing	179:187	Sanyeqing	179:187	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	10	92	from	400 mg/kg	1664:1672	arg1	administration					1630:1643	The oral administration	1621:1643	The oral administration of SYQP at 200 and 400 mg/kg	1621:1672	The oral administration of SYQP at 200 and 400 mg/kg could markedly suppress the hyperthermia of mice induced by Brewer's yeast and decrease the production of cytokines especially prostaglandin E2 (PGE2) in the serum of mice.
32045682	7	93	theme	H22	1276:1278	arg1	mice					1294:1297	H22 tumor bearing mice	1276:1297	H22 tumor bearing mice	1276:1297	Antitumor effect was investigated using H22 tumor bearing mice.
32045682	11	94	theme	cytokines	1996:2004	arg1	production					1982:1991	the production	1978:1991	the production of cytokines such as tumor necrosis factor-alpha (TNF-a) and interferon γ (IFN-γ)	1978:2073	SYQP inhibited the growth of H22 tumor in mice with inhibitory rate of 39.9% at the administration dose of 200 mg/kg and increased the production of cytokines such as tumor necrosis factor-alpha (TNF-a) and interferon γ (IFN-γ).
32045682	2	95	theme	sea	592:594	arg1	level					596:600	the sea level	588:600	the sea level	588:600	It belongs to the family of Vitaceae, and is mainly distributed in southeast China (Yunnan province) and can be found in India (Andaman Islands), Myanmar, Thailand, Vietnam, Malaysia and Indonesia in the valleys with 1100-1300 m above the sea level.
32045682	1	96	theme	hepatitis	279:287	arg1	treatments					239:248	the treatments	235:248	the treatments of inflammation, high fever, hepatitis and cancer	235:298	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	1	97	theme	cancer	293:298	arg1	treatments					239:248	the treatments	235:248	the treatments of inflammation, high fever, hepatitis and cancer	235:298	ETHNOPHARMACOLOGICAL RELEVANCE Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing) is traditionally used as a folk medicine for the treatments of inflammation, high fever, hepatitis and cancer, and can improve the immune function of the patient.
32045682	11	98	theme	necrosis	2020:2027	arg1	TNF-a					2043:2047	TNF-a	2043:2047	TNF-a	2043:2047	SYQP inhibited the growth of H22 tumor in mice with inhibitory rate of 39.9% at the administration dose of 200 mg/kg and increased the production of cytokines such as tumor necrosis factor-alpha (TNF-a) and interferon γ (IFN-γ).
32045682	11	98	theme	necrosis	2020:2027	arg1	factor-alpha					2029:2040	tumor necrosis factor-alpha	2014:2040	tumor necrosis factor-alpha (TNF-a)	2014:2048	SYQP inhibited the growth of H22 tumor in mice with inhibitory rate of 39.9% at the administration dose of 200 mg/kg and increased the production of cytokines such as tumor necrosis factor-alpha (TNF-a) and interferon γ (IFN-γ).
32045682	10	99	theme	mice	1718:1721	arg1	hyperthermia					1702:1713	the hyperthermia	1698:1713	the hyperthermia of mice induced by Brewer's yeast	1698:1747	The oral administration of SYQP at 200 and 400 mg/kg could markedly suppress the hyperthermia of mice induced by Brewer's yeast and decrease the production of cytokines especially prostaglandin E2 (PGE2) in the serum of mice.
32045682	11	100	theme	interferon	2054:2063	arg1	IFN-γ					2068:2072	IFN-γ	2068:2072	IFN-γ	2068:2072	SYQP inhibited the growth of H22 tumor in mice with inhibitory rate of 39.9% at the administration dose of 200 mg/kg and increased the production of cytokines such as tumor necrosis factor-alpha (TNF-a) and interferon γ (IFN-γ).
32045682	11	100	theme	interferon	2054:2063	arg1	γ					2065:2065	interferon γ	2054:2065	interferon γ (IFN-γ)	2054:2073	SYQP inhibited the growth of H22 tumor in mice with inhibitory rate of 39.9% at the administration dose of 200 mg/kg and increased the production of cytokines such as tumor necrosis factor-alpha (TNF-a) and interferon γ (IFN-γ).
32045682	0	101	theme	polysaccharide	48:61	arg1	effects					26:32	Antipyretic and antitumor effects	0:32	Antipyretic and antitumor effects of a purified polysaccharide from aerial parts of Tetrastigma hemsleyanum.	0:107	Antipyretic and antitumor effects of a purified polysaccharide from aerial parts of Tetrastigma hemsleyanum.
32045682	4	102	theme	Tetrastigma	931:941	arg1	hemsleyanum					943:953	Tetrastigma hemsleyanum	931:953	Tetrastigma hemsleyanum	931:953	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of Tetrastigma hemsleyanum were extracted and fractionated by DEAE and gel permeation chromatography.
32045682	9	103	contain	had	1576:1578	arg2	weight					1601:1606	an average molecular weight	1580:1606	an average molecular weight of 66.2 kDa	1580:1618	RESULTS SYQP was composed of galacturonic acid (GalA), glucose (Glc), mannose (Man), arabinose (Ara), galactose (Gal), and rhamnose (Rha) with a molar ratio of 11.3:7.1:2.5:1.0:0.9:0.5 and it had an average molecular weight of 66.2 kDa.
32045682	9	103	contain	had	1576:1578	arg1	it					1573:1574	it	1573:1574	it	1573:1574	RESULTS SYQP was composed of galacturonic acid (GalA), glucose (Glc), mannose (Man), arabinose (Ara), galactose (Gal), and rhamnose (Rha) with a molar ratio of 11.3:7.1:2.5:1.0:0.9:0.5 and it had an average molecular weight of 66.2 kDa.
32045682	13	104	theme	function	2513:2520	arg1	regulation					2487:2496	its regulation	2483:2496	its regulation of host immune function by controlling the secretion of cytokines	2483:2562	CONCLUSION The anti-inflammatory and antitumor activities of SYQP might be related to its regulation of host immune function by controlling the secretion of cytokines.
32045682	4	105	from	parts	922:926	arg1	METHODS					862:868	METHODS	862:868	METHODS	862:868	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of Tetrastigma hemsleyanum were extracted and fractionated by DEAE and gel permeation chromatography.
32045682	4	105	from	parts	922:926	arg1	MATERIALS					848:856	MATERIALS	848:856	MATERIALS	848:856	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of Tetrastigma hemsleyanum were extracted and fractionated by DEAE and gel permeation chromatography.
32045682	2	106	located	found	465:469	arg1	valleys					557:563	the valleys	553:563	the valleys with 1100-1300 m above the sea level	553:600	It belongs to the family of Vitaceae, and is mainly distributed in southeast China (Yunnan province) and can be found in India (Andaman Islands), Myanmar, Thailand, Vietnam, Malaysia and Indonesia in the valleys with 1100-1300 m above the sea level.
32045682	2	106	located	found	465:469	arg1	Malaysia					527:534	Malaysia	527:534	Malaysia	527:534	It belongs to the family of Vitaceae, and is mainly distributed in southeast China (Yunnan province) and can be found in India (Andaman Islands), Myanmar, Thailand, Vietnam, Malaysia and Indonesia in the valleys with 1100-1300 m above the sea level.
32045682	2	106	located	found	465:469	arg1	Islands					489:495	Islands	489:495	Islands	489:495	It belongs to the family of Vitaceae, and is mainly distributed in southeast China (Yunnan province) and can be found in India (Andaman Islands), Myanmar, Thailand, Vietnam, Malaysia and Indonesia in the valleys with 1100-1300 m above the sea level.
32045682	2	106	located	found	465:469	arg1	India					474:478	India	474:478	India	474:478	It belongs to the family of Vitaceae, and is mainly distributed in southeast China (Yunnan province) and can be found in India (Andaman Islands), Myanmar, Thailand, Vietnam, Malaysia and Indonesia in the valleys with 1100-1300 m above the sea level.
32045682	2	106	located	found	465:469	arg1	Myanmar					499:505	Myanmar	499:505	Myanmar	499:505	It belongs to the family of Vitaceae, and is mainly distributed in southeast China (Yunnan province) and can be found in India (Andaman Islands), Myanmar, Thailand, Vietnam, Malaysia and Indonesia in the valleys with 1100-1300 m above the sea level.
32045682	2	106	located	found	465:469	arg1	Thailand					508:515	Thailand	508:515	Thailand	508:515	It belongs to the family of Vitaceae, and is mainly distributed in southeast China (Yunnan province) and can be found in India (Andaman Islands), Myanmar, Thailand, Vietnam, Malaysia and Indonesia in the valleys with 1100-1300 m above the sea level.
32045682	2	106	located	found	465:469	arg1	Indonesia					540:548	Indonesia	540:548	Indonesia	540:548	It belongs to the family of Vitaceae, and is mainly distributed in southeast China (Yunnan province) and can be found in India (Andaman Islands), Myanmar, Thailand, Vietnam, Malaysia and Indonesia in the valleys with 1100-1300 m above the sea level.
32045682	2	106	located	found	465:469	arg2	It					353:354	It	353:354	It	353:354	It belongs to the family of Vitaceae, and is mainly distributed in southeast China (Yunnan province) and can be found in India (Andaman Islands), Myanmar, Thailand, Vietnam, Malaysia and Indonesia in the valleys with 1100-1300 m above the sea level.
32045682	2	106	located	found	465:469	arg1	Vietnam					518:524	Vietnam	518:524	Vietnam	518:524	It belongs to the family of Vitaceae, and is mainly distributed in southeast China (Yunnan province) and can be found in India (Andaman Islands), Myanmar, Thailand, Vietnam, Malaysia and Indonesia in the valleys with 1100-1300 m above the sea level.
32045682	4	107	theme	permeation	1003:1012	arg1	chromatography					1014:1027	DEAE and gel permeation chromatography	990:1027	chromatography	1014:1027	MATERIALS AND METHODS Water-soluble crude polysaccharides from the aerial parts of Tetrastigma hemsleyanum were extracted and fractionated by DEAE and gel permeation chromatography.
32045682	8	108	theme	serum	1304:1308	arg1	cytokines					1310:1318	The serum cytokines	1300:1318	The serum cytokines	1300:1318	The serum cytokines were determined to evaluated the biological activities of SYQP.
32045682	8	109	theme	SYQP	1378:1381	arg1	activities					1364:1373	the biological activities	1349:1373	the biological activities of SYQP	1349:1381	The serum cytokines were determined to evaluated the biological activities of SYQP.
32755709	3	0	theme	molecular	294:302	arg1	mechanism					304:312	its molecular mechanism	290:312	its molecular mechanism	290:312	spinosa seeds (ZSS) polysaccharides on the bioavailability of spinosin in mice and its molecular mechanism were investigated.
32755709	9	1	theme	inflammatory	1155:1166	arg1	infiltration					1173:1184	inflammatory cell infiltration	1155:1184	inflammatory cell infiltration in mice	1155:1192	Furthermore, histopathological analysis revealed ZSS polysaccharides can alleviate TNBS-induced colitis, reduced inflammatory cell infiltration in mice.
32755709	3	2	theme	seeds	215:219	arg1	polysaccharides					227:241	spinosa seeds (ZSS) polysaccharides	207:241	spinosa seeds (ZSS) polysaccharides on the bioavailability of spinosin in mice and its molecular mechanism	207:312	spinosa seeds (ZSS) polysaccharides on the bioavailability of spinosin in mice and its molecular mechanism were investigated.
32755709	6	3	theme	Bacteroidetes	715:727	arg1	abundance					702:710	the abundance	698:710	the abundance of Bacteroidetes	698:727	ZSS polysaccharides significantly regulated the composition of the gut microbiota, reducing the abundance of Bacteroidetes, and increasing the richness of Firmicutes and Verrucomicrobia.
32755709	7	4	theme	transporters	910:921	arg1	levels					865:870	the expression levels	850:870	the expression levels of tight junction proteins and efflux transporters in Caco-2 cells	850:937	Moreover, ZSS polysaccharides can significantly regulate the expression levels of tight junction proteins and efflux transporters in Caco-2 cells.
32755709	10	5	theme	RAW264.7	1264:1271	arg1	cells					1273:1277	RAW264.7 cells	1264:1277	RAW264.7 cells	1264:1277	This immune regulation was related to the NF-κB and MAPK pathways in RAW264.7 cells.
32755709	0	6	from	mechanisms	10:19	arg1	var					66:68	Ziziphus jujuba Mill var	45:68	Ziziphus jujuba Mill var	45:68	Molecular mechanisms of polysaccharides from Ziziphus jujuba Mill var.
32755709	8	7	theme	obvious	998:1004	arg1	activity					1017:1024	no obvious biological activity	995:1024	no obvious biological activity	995:1024	However, the gut microbiota culture supernatant showed no obvious biological activity in this regard.
32755709	6	8	theme	ZSS	606:608	arg1	polysaccharides					610:624	ZSS polysaccharides	606:624	ZSS polysaccharides	606:624	ZSS polysaccharides significantly regulated the composition of the gut microbiota, reducing the abundance of Bacteroidetes, and increasing the richness of Firmicutes and Verrucomicrobia.
32755709	3	9	from	bioavailability	250:264	arg1	mice					281:284	mice	281:284	mice	281:284	spinosa seeds (ZSS) polysaccharides on the bioavailability of spinosin in mice and its molecular mechanism were investigated.
32755709	3	9	from	bioavailability	250:264	arg1	mechanism					304:312	its molecular mechanism	290:312	its molecular mechanism	290:312	spinosa seeds (ZSS) polysaccharides on the bioavailability of spinosin in mice and its molecular mechanism were investigated.
32755709	4	10	theme	control	499:505	arg1	group					507:511	the control group	495:511	the control group	495:511	After continuously fed with ZSS polysaccharides 100 mg/kg·d-1 for 28 consecutive days, the C57BL/6 mice absorbed spinosin at an obvious lower level compared with the control group.
32755709	3	11	theme	spinosa	207:213	arg1	polysaccharides					227:241	spinosa seeds (ZSS) polysaccharides	207:241	spinosa seeds (ZSS) polysaccharides on the bioavailability of spinosin in mice and its molecular mechanism	207:312	spinosa seeds (ZSS) polysaccharides on the bioavailability of spinosin in mice and its molecular mechanism were investigated.
32755709	9	12	theme	cell	1168:1171	arg1	infiltration					1173:1184	inflammatory cell infiltration	1155:1184	inflammatory cell infiltration in mice	1155:1192	Furthermore, histopathological analysis revealed ZSS polysaccharides can alleviate TNBS-induced colitis, reduced inflammatory cell infiltration in mice.
32755709	7	13	theme	tight	875:879	arg1	proteins					890:897	tight junction proteins	875:897	tight junction proteins	875:897	Moreover, ZSS polysaccharides can significantly regulate the expression levels of tight junction proteins and efflux transporters in Caco-2 cells.
32755709	10	14	theme	MAPK	1247:1250	arg1	pathways					1252:1259	the NF-κB and MAPK pathways	1233:1259	the NF-κB and MAPK pathways in RAW264.7 cells	1233:1277	This immune regulation was related to the NF-κB and MAPK pathways in RAW264.7 cells.
32755709	4	15	theme	obvious	461:467	arg1	level					475:479	an obvious lower level	458:479	an obvious lower level	458:479	After continuously fed with ZSS polysaccharides 100 mg/kg·d-1 for 28 consecutive days, the C57BL/6 mice absorbed spinosin at an obvious lower level compared with the control group.
32755709	4	16	theme	consecutive	402:412	arg1	days					414:417	28 consecutive days	399:417	28 consecutive days	399:417	After continuously fed with ZSS polysaccharides 100 mg/kg·d-1 for 28 consecutive days, the C57BL/6 mice absorbed spinosin at an obvious lower level compared with the control group.
32755709	1	17	theme	spinosa	71:77	arg1	seeds					79:83	spinosa seeds	71:83	spinosa seeds	71:83	spinosa seeds regulating the bioavailability of spinosin and preventing colitis.
32755709	7	18	theme	junction	881:888	arg1	proteins					890:897	tight junction proteins	875:897	tight junction proteins	875:897	Moreover, ZSS polysaccharides can significantly regulate the expression levels of tight junction proteins and efflux transporters in Caco-2 cells.
32755709	4	19	theme	ZSS	361:363	arg1	100 mg/kg·d-1					381:393	ZSS polysaccharides 100 mg/kg·d-1	361:393	ZSS polysaccharides 100 mg/kg·d-1	361:393	After continuously fed with ZSS polysaccharides 100 mg/kg·d-1 for 28 consecutive days, the C57BL/6 mice absorbed spinosin at an obvious lower level compared with the control group.
32755709	5	20	from	levels	529:534	arg1	colon					570:574	the colon	566:574	the colon	566:574	The expression levels of P-gp, MRP2 and Occludin in the colon were significantly increased.
32755709	7	21	theme	ZSS	803:805	arg1	polysaccharides					807:821	ZSS polysaccharides	803:821	ZSS polysaccharides	803:821	Moreover, ZSS polysaccharides can significantly regulate the expression levels of tight junction proteins and efflux transporters in Caco-2 cells.
32755709	3	22	theme	ZSS	222:224	arg1	polysaccharides					227:241	spinosa seeds (ZSS) polysaccharides	207:241	spinosa seeds (ZSS) polysaccharides on the bioavailability of spinosin in mice and its molecular mechanism	207:312	spinosa seeds (ZSS) polysaccharides on the bioavailability of spinosin in mice and its molecular mechanism were investigated.
32755709	5	23	theme	Occludin	554:561	arg1	levels					529:534	The expression levels	514:534	The expression levels of P-gp, MRP2 and Occludin in the colon	514:574	The expression levels of P-gp, MRP2 and Occludin in the colon were significantly increased.
32755709	9	24	theme	ZSS	1091:1093	arg1	polysaccharides					1095:1109	ZSS polysaccharides	1091:1109	ZSS polysaccharides	1091:1109	Furthermore, histopathological analysis revealed ZSS polysaccharides can alleviate TNBS-induced colitis, reduced inflammatory cell infiltration in mice.
32755709	0	25	theme	Molecular	0:8	arg1	mechanisms					10:19	Molecular mechanisms	0:19	Molecular mechanisms of polysaccharides from Ziziphus jujuba Mill var.	0:69	Molecular mechanisms of polysaccharides from Ziziphus jujuba Mill var.
32755709	2	26	theme	Ziziphus	181:188	arg1	effect					171:176	the effect	167:176	the effect of Ziziphus	167:188	In this study, the effect of Ziziphus jujuba Mill var.
32755709	6	27	theme	Verrucomicrobia	776:790	arg1	richness					749:756	the richness	745:756	the richness of Firmicutes and Verrucomicrobia	745:790	ZSS polysaccharides significantly regulated the composition of the gut microbiota, reducing the abundance of Bacteroidetes, and increasing the richness of Firmicutes and Verrucomicrobia.
32755709	0	28	theme	polysaccharides	24:38	arg1	mechanisms					10:19	Molecular mechanisms	0:19	Molecular mechanisms of polysaccharides from Ziziphus jujuba Mill var.	0:69	Molecular mechanisms of polysaccharides from Ziziphus jujuba Mill var.
32755709	9	29	dep	revealed	1082:1089	arg1	reduced					1147:1153	reduced	1147:1153	reduced inflammatory cell infiltration in mice	1147:1192	Furthermore, histopathological analysis revealed ZSS polysaccharides can alleviate TNBS-induced colitis, reduced inflammatory cell infiltration in mice.
32755709	4	30	theme	polysaccharides	365:379	arg1	100 mg/kg·d-1					381:393	ZSS polysaccharides 100 mg/kg·d-1	361:393	ZSS polysaccharides 100 mg/kg·d-1	361:393	After continuously fed with ZSS polysaccharides 100 mg/kg·d-1 for 28 consecutive days, the C57BL/6 mice absorbed spinosin at an obvious lower level compared with the control group.
32755709	6	31	theme	microbiota	677:686	arg1	composition					654:664	the composition	650:664	the composition of the gut microbiota	650:686	ZSS polysaccharides significantly regulated the composition of the gut microbiota, reducing the abundance of Bacteroidetes, and increasing the richness of Firmicutes and Verrucomicrobia.
32755709	8	32	theme	microbiota	957:966	arg1	supernatant					976:986	the gut microbiota culture supernatant	949:986	the gut microbiota culture supernatant	949:986	However, the gut microbiota culture supernatant showed no obvious biological activity in this regard.
32755709	3	33	from	polysaccharides	227:241	arg1	bioavailability					250:264	the bioavailability	246:264	the bioavailability of spinosin in mice and its molecular mechanism	246:312	spinosa seeds (ZSS) polysaccharides on the bioavailability of spinosin in mice and its molecular mechanism were investigated.
32755709	3	34	theme	spinosin	269:276	arg1	bioavailability					250:264	the bioavailability	246:264	the bioavailability of spinosin in mice and its molecular mechanism	246:312	spinosa seeds (ZSS) polysaccharides on the bioavailability of spinosin in mice and its molecular mechanism were investigated.
32755709	6	35	theme	gut	673:675	arg1	microbiota					677:686	the gut microbiota	669:686	the gut microbiota	669:686	ZSS polysaccharides significantly regulated the composition of the gut microbiota, reducing the abundance of Bacteroidetes, and increasing the richness of Firmicutes and Verrucomicrobia.
32755709	0	36	theme	Ziziphus	45:52	arg1	var					66:68	Ziziphus jujuba Mill var	45:68	Ziziphus jujuba Mill var	45:68	Molecular mechanisms of polysaccharides from Ziziphus jujuba Mill var.
32755709	4	37	dep	obvious	461:467	arg1	lower					469:473	lower	469:473	lower	469:473	After continuously fed with ZSS polysaccharides 100 mg/kg·d-1 for 28 consecutive days, the C57BL/6 mice absorbed spinosin at an obvious lower level compared with the control group.
32755709	7	38	theme	proteins	890:897	arg1	levels					865:870	the expression levels	850:870	the expression levels of tight junction proteins and efflux transporters in Caco-2 cells	850:937	Moreover, ZSS polysaccharides can significantly regulate the expression levels of tight junction proteins and efflux transporters in Caco-2 cells.
32755709	9	39	from	infiltration	1173:1184	arg1	mice					1189:1192	mice	1189:1192	mice	1189:1192	Furthermore, histopathological analysis revealed ZSS polysaccharides can alleviate TNBS-induced colitis, reduced inflammatory cell infiltration in mice.
32755709	6	40	theme	Firmicutes	761:770	arg1	richness					749:756	the richness	745:756	the richness of Firmicutes and Verrucomicrobia	745:790	ZSS polysaccharides significantly regulated the composition of the gut microbiota, reducing the abundance of Bacteroidetes, and increasing the richness of Firmicutes and Verrucomicrobia.
32755709	0	41	theme	Mill	61:64	arg1	var					66:68	Ziziphus jujuba Mill var	45:68	Ziziphus jujuba Mill var	45:68	Molecular mechanisms of polysaccharides from Ziziphus jujuba Mill var.
32755709	10	42	from	pathways	1252:1259	arg1	cells					1273:1277	RAW264.7 cells	1264:1277	RAW264.7 cells	1264:1277	This immune regulation was related to the NF-κB and MAPK pathways in RAW264.7 cells.
32755709	0	43	from	var	66:68	arg1	polysaccharides					24:38	polysaccharides	24:38	polysaccharides from Ziziphus jujuba Mill var	24:68	Molecular mechanisms of polysaccharides from Ziziphus jujuba Mill var.
32755709	0	43	from	var	66:68	arg1	mechanisms					10:19	Molecular mechanisms	0:19	Molecular mechanisms of polysaccharides from Ziziphus jujuba Mill var.	0:69	Molecular mechanisms of polysaccharides from Ziziphus jujuba Mill var.
32755709	5	44	theme	P-gp	539:542	arg1	levels					529:534	The expression levels	514:534	The expression levels of P-gp, MRP2 and Occludin in the colon	514:574	The expression levels of P-gp, MRP2 and Occludin in the colon were significantly increased.
32755709	1	45	theme	spinosin	119:126	arg1	bioavailability					100:114	the bioavailability	96:114	the bioavailability of spinosin	96:126	spinosa seeds regulating the bioavailability of spinosin and preventing colitis.
32755709	0	46	theme	jujuba	54:59	arg1	var					66:68	Ziziphus jujuba Mill var	45:68	Ziziphus jujuba Mill var	45:68	Molecular mechanisms of polysaccharides from Ziziphus jujuba Mill var.
32755709	8	47	theme	gut	953:955	arg1	supernatant					976:986	the gut microbiota culture supernatant	949:986	the gut microbiota culture supernatant	949:986	However, the gut microbiota culture supernatant showed no obvious biological activity in this regard.
32755709	9	48	theme	TNBS-induced	1125:1136	arg1	colitis					1138:1144	TNBS-induced colitis	1125:1144	TNBS-induced colitis	1125:1144	Furthermore, histopathological analysis revealed ZSS polysaccharides can alleviate TNBS-induced colitis, reduced inflammatory cell infiltration in mice.
32755709	10	49	theme	immune	1200:1205	arg1	regulation					1207:1216	This immune regulation	1195:1216	This immune regulation	1195:1216	This immune regulation was related to the NF-κB and MAPK pathways in RAW264.7 cells.
32755709	2	50	theme	Mill	197:200	arg1	var					202:204	Mill var	197:204	Mill var	197:204	In this study, the effect of Ziziphus jujuba Mill var.
32755709	7	51	from	levels	865:870	arg1	cells					933:937	Caco-2 cells	926:937	Caco-2 cells	926:937	Moreover, ZSS polysaccharides can significantly regulate the expression levels of tight junction proteins and efflux transporters in Caco-2 cells.
32755709	5	52	theme	MRP2	545:548	arg1	levels					529:534	The expression levels	514:534	The expression levels of P-gp, MRP2 and Occludin in the colon	514:574	The expression levels of P-gp, MRP2 and Occludin in the colon were significantly increased.
32755709	8	53	theme	biological	1006:1015	arg1	activity					1017:1024	no obvious biological activity	995:1024	no obvious biological activity	995:1024	However, the gut microbiota culture supernatant showed no obvious biological activity in this regard.
32755709	8	54	theme	culture	968:974	arg1	supernatant					976:986	the gut microbiota culture supernatant	949:986	the gut microbiota culture supernatant	949:986	However, the gut microbiota culture supernatant showed no obvious biological activity in this regard.
32755709	7	55	theme	expression	854:863	arg1	levels					865:870	the expression levels	850:870	the expression levels of tight junction proteins and efflux transporters in Caco-2 cells	850:937	Moreover, ZSS polysaccharides can significantly regulate the expression levels of tight junction proteins and efflux transporters in Caco-2 cells.
32755709	9	56	theme	histopathological	1055:1071	arg1	analysis					1073:1080	histopathological analysis	1055:1080	histopathological analysis	1055:1080	Furthermore, histopathological analysis revealed ZSS polysaccharides can alleviate TNBS-induced colitis, reduced inflammatory cell infiltration in mice.
32755709	10	57	theme	NF-κB	1237:1241	arg1	pathways					1252:1259	the NF-κB and MAPK pathways	1233:1259	the NF-κB and MAPK pathways in RAW264.7 cells	1233:1277	This immune regulation was related to the NF-κB and MAPK pathways in RAW264.7 cells.
32755709	4	58	theme	C57BL/6	424:430	arg1	mice					432:435	the C57BL/6 mice	420:435	the C57BL/6 mice	420:435	After continuously fed with ZSS polysaccharides 100 mg/kg·d-1 for 28 consecutive days, the C57BL/6 mice absorbed spinosin at an obvious lower level compared with the control group.
32755709	6	59	dep	regulated	640:648	arg1	increasing					734:743	increasing	734:743	increasing the richness of Firmicutes and Verrucomicrobia	734:790	ZSS polysaccharides significantly regulated the composition of the gut microbiota, reducing the abundance of Bacteroidetes, and increasing the richness of Firmicutes and Verrucomicrobia.
32755709	6	59	dep	regulated	640:648	arg1	reducing					689:696	reducing	689:696	reducing the abundance of Bacteroidetes	689:727	ZSS polysaccharides significantly regulated the composition of the gut microbiota, reducing the abundance of Bacteroidetes, and increasing the richness of Firmicutes and Verrucomicrobia.
32755709	7	60	theme	Caco-2	926:931	arg1	cells					933:937	Caco-2 cells	926:937	Caco-2 cells	926:937	Moreover, ZSS polysaccharides can significantly regulate the expression levels of tight junction proteins and efflux transporters in Caco-2 cells.
32755709	5	61	theme	expression	518:527	arg1	levels					529:534	The expression levels	514:534	The expression levels of P-gp, MRP2 and Occludin in the colon	514:574	The expression levels of P-gp, MRP2 and Occludin in the colon were significantly increased.
32755709	7	62	theme	efflux	903:908	arg1	transporters					910:921	efflux transporters	903:921	efflux transporters	903:921	Moreover, ZSS polysaccharides can significantly regulate the expression levels of tight junction proteins and efflux transporters in Caco-2 cells.
33229155	8	0	theme	least	1082:1086	arg1	analysis					1112:1119	partial least square regression (PLS) analysis	1074:1119	partial least square regression (PLS) analysis	1074:1119	Thus, a model was built using partial least square regression (PLS) analysis to predict the palate fullness intensity in beers (R2C = 0.7993).
33229155	7	1	theme	distribution	977:988	arg1	nitrogen					997:1004	total nitrogen	991:1004	total nitrogen (p < 0.001)	991:1016	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	7	1	theme	distribution	977:988	arg1	β-glucan					1023:1030	β-glucan	1023:1030	β-glucan (p < 0.01)	1023:1041	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	7	1	theme	distribution	977:988	arg1	indices					951:957	indices	951:957	indices of macromolecular distribution	951:988	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	7	1	theme	distribution	977:988	arg1	p < 0.01					1033:1040	p < 0.01	1033:1040	p < 0.01	1033:1040	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	7	1	theme	distribution	977:988	arg1	p < 0.001					1007:1015	p < 0.001	1007:1015	p < 0.001	1007:1015	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	7	1	theme	distribution	977:988	arg1	gravity					931:937	original gravity	922:937	original gravity	922:937	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	7	1	theme	distribution	977:988	arg1	viscosity					940:948	viscosity	940:948	viscosity	940:948	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	0	2	from	modeling	12:19	arg1	lager					43:47	lager	43:47	lager	43:47	Chemometric modeling of palate fullness in lager beers.
33229155	8	3	theme	partial	1074:1080	arg1	analysis					1112:1119	partial least square regression (PLS) analysis	1074:1119	partial least square regression (PLS) analysis	1074:1119	Thus, a model was built using partial least square regression (PLS) analysis to predict the palate fullness intensity in beers (R2C = 0.7993).
33229155	7	4	dep	found	830:834	arg1	nitrogen					997:1004	total nitrogen	991:1004	total nitrogen (p < 0.001)	991:1016	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	7	4	dep	found	830:834	arg1	β-glucan					1023:1030	β-glucan	1023:1030	β-glucan (p < 0.01)	1023:1041	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	7	4	dep	found	830:834	arg1	indices					951:957	indices	951:957	indices of macromolecular distribution	951:988	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	7	4	dep	found	830:834	arg1	p < 0.01					1033:1040	p < 0.01	1033:1040	p < 0.01	1033:1040	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	7	4	dep	found	830:834	arg1	p < 0.001					1007:1015	p < 0.001	1007:1015	p < 0.001	1007:1015	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	7	4	dep	found	830:834	arg1	gravity					931:937	original gravity	922:937	original gravity	922:937	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	7	4	dep	found	830:834	arg1	viscosity					940:948	viscosity	940:948	viscosity	940:948	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	9	5	theme	palate	1247:1252	arg1	fullness					1254:1261	control palate fullness	1239:1261	control palate fullness	1239:1261	This model can be used as a guideline by brewers to control palate fullness and mouthfeel.
33229155	4	6	theme	compositions	513:524	arg1	data					500:503	analytical data	489:503	analytical data of beer compositions, palate fullness, and mouthfeel descriptors	489:568	This article reports a chemometric analysis of 41 lager beers by evaluating analytical data of beer compositions, palate fullness, and mouthfeel descriptors.
33229155	8	7	theme	regression	1095:1104	arg1	analysis					1112:1119	partial least square regression (PLS) analysis	1074:1119	partial least square regression (PLS) analysis	1074:1119	Thus, a model was built using partial least square regression (PLS) analysis to predict the palate fullness intensity in beers (R2C = 0.7993).
33229155	5	8	theme	macromolecular	618:631	arg1	compositions					633:644	the macromolecular compositions	614:644	the macromolecular compositions of classical lager beers	614:669	AF4-MALS-dRI indicated high variability in the macromolecular compositions of classical lager beers.
33229155	3	9	theme	concentration	372:384	arg1	range					386:390	the concentration range	368:390	the concentration range found in lager beer	368:410	However, systematic correlations between sensory attributes and native beer compounds have not been evaluated within the concentration range found in lager beer.
33229155	2	10	theme	Non-volatile	138:149	arg1	substances					151:160	Non-volatile substances	138:160	Non-volatile substances	138:160	Non-volatile substances and molar mass fractions influence sensory perceptions of palate fullness and mouthfeel.
33229155	8	11	theme	square	1088:1093	arg1	analysis					1112:1119	partial least square regression (PLS) analysis	1074:1119	partial least square regression (PLS) analysis	1074:1119	Thus, a model was built using partial least square regression (PLS) analysis to predict the palate fullness intensity in beers (R2C = 0.7993).
33229155	1	12	theme	Palate	56:61	arg1	fullness					63:70	Palate fullness	56:70	Palate fullness	56:70	Palate fullness and mouthfeel of beer are key attributes of sensory beer quality.
33229155	1	12	theme	Palate	56:61	arg1	attributes					102:111	key attributes	98:111	key attributes of sensory beer quality	98:135	Palate fullness and mouthfeel of beer are key attributes of sensory beer quality.
33229155	1	12	theme	Palate	56:61	arg1	mouthfeel					76:84	mouthfeel	76:84	mouthfeel	76:84	Palate fullness and mouthfeel of beer are key attributes of sensory beer quality.
33229155	5	13	from	variability	599:609	arg1	compositions					633:644	the macromolecular compositions	614:644	the macromolecular compositions of classical lager beers	614:669	AF4-MALS-dRI indicated high variability in the macromolecular compositions of classical lager beers.
33229155	5	14	theme	classical	649:657	arg1	beers					665:669	classical lager beers	649:669	classical lager beers	649:669	AF4-MALS-dRI indicated high variability in the macromolecular compositions of classical lager beers.
33229155	6	15	theme	fullness	748:755	arg1	intensity					757:765	palate fullness intensity	741:765	palate fullness intensity	741:765	Screened beers were clustered into groups differing significantly in palate fullness intensity and macromolecular distribution.
33229155	7	16	dep	fullness	851:858	arg1	parameters					910:919	composition parameters	898:919	composition parameters	898:919	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	5	17	theme	lager	659:663	arg1	beers					665:669	classical lager beers	649:669	classical lager beers	649:669	AF4-MALS-dRI indicated high variability in the macromolecular compositions of classical lager beers.
33229155	0	18	theme	Chemometric	0:10	arg1	modeling					12:19	Chemometric modeling	0:19	Chemometric modeling of palate fullness in lager	0:47	Chemometric modeling of palate fullness in lager beers.
33229155	6	19	theme	palate	741:746	arg1	intensity					757:765	palate fullness intensity	741:765	palate fullness intensity	741:765	Screened beers were clustered into groups differing significantly in palate fullness intensity and macromolecular distribution.
33229155	4	20	theme	analytical	489:498	arg1	data					500:503	analytical data	489:503	analytical data of beer compositions, palate fullness, and mouthfeel descriptors	489:568	This article reports a chemometric analysis of 41 lager beers by evaluating analytical data of beer compositions, palate fullness, and mouthfeel descriptors.
33229155	5	21	theme	beers	665:669	arg1	compositions					633:644	the macromolecular compositions	614:644	the macromolecular compositions of classical lager beers	614:669	AF4-MALS-dRI indicated high variability in the macromolecular compositions of classical lager beers.
33229155	5	22	theme	high	594:597	arg1	variability					599:609	high variability	594:609	high variability in the macromolecular compositions of classical lager beers	594:669	AF4-MALS-dRI indicated high variability in the macromolecular compositions of classical lager beers.
33229155	8	23	theme	fullness	1143:1150	arg1	intensity					1152:1160	the palate fullness intensity	1132:1160	the palate fullness intensity	1132:1160	Thus, a model was built using partial least square regression (PLS) analysis to predict the palate fullness intensity in beers (R2C = 0.7993).
33229155	3	24	theme	lager	401:405	arg1	beer					407:410	lager beer	401:410	lager beer	401:410	However, systematic correlations between sensory attributes and native beer compounds have not been evaluated within the concentration range found in lager beer.
33229155	4	25	theme	descriptors	558:568	arg1	data					500:503	analytical data	489:503	analytical data of beer compositions, palate fullness, and mouthfeel descriptors	489:568	This article reports a chemometric analysis of 41 lager beers by evaluating analytical data of beer compositions, palate fullness, and mouthfeel descriptors.
33229155	8	26	theme	PLS	1107:1109	arg1	analysis					1112:1119	partial least square regression (PLS) analysis	1074:1119	partial least square regression (PLS) analysis	1074:1119	Thus, a model was built using partial least square regression (PLS) analysis to predict the palate fullness intensity in beers (R2C = 0.7993).
33229155	0	27	theme	palate	24:29	arg1	fullness					31:38	palate fullness	24:38	palate fullness	24:38	Chemometric modeling of palate fullness in lager beers.
33229155	4	28	theme	mouthfeel	548:556	arg1	descriptors					558:568	mouthfeel descriptors	548:568	mouthfeel descriptors	548:568	This article reports a chemometric analysis of 41 lager beers by evaluating analytical data of beer compositions, palate fullness, and mouthfeel descriptors.
33229155	9	29	theme	control	1239:1245	arg1	fullness					1254:1261	control palate fullness	1239:1261	control palate fullness	1239:1261	This model can be used as a guideline by brewers to control palate fullness and mouthfeel.
33229155	1	30	theme	beer	89:92	arg1	fullness					63:70	Palate fullness	56:70	Palate fullness	56:70	Palate fullness and mouthfeel of beer are key attributes of sensory beer quality.
33229155	1	30	theme	beer	89:92	arg1	attributes					102:111	key attributes	98:111	key attributes of sensory beer quality	98:135	Palate fullness and mouthfeel of beer are key attributes of sensory beer quality.
33229155	1	30	theme	beer	89:92	arg1	mouthfeel					76:84	mouthfeel	76:84	mouthfeel	76:84	Palate fullness and mouthfeel of beer are key attributes of sensory beer quality.
33229155	7	31	theme	original	922:929	arg1	gravity					931:937	original gravity	922:937	original gravity	922:937	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	7	32	theme	macromolecular	864:877	arg1	fractions					879:887	macromolecular fractions	864:887	macromolecular fractions	864:887	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	3	33	theme	sensory	292:298	arg1	attributes					300:309	sensory attributes	292:309	sensory attributes	292:309	However, systematic correlations between sensory attributes and native beer compounds have not been evaluated within the concentration range found in lager beer.
33229155	2	34	theme	mass	172:175	arg1	fractions					177:185	molar mass fractions	166:185	molar mass fractions	166:185	Non-volatile substances and molar mass fractions influence sensory perceptions of palate fullness and mouthfeel.
33229155	9	35	used	used	1205:1208	arg2	model					1192:1196	This model	1187:1196	This model	1187:1196	This model can be used as a guideline by brewers to control palate fullness and mouthfeel.
33229155	9	35	used	used	1205:1208	arg2	guideline					1215:1223	a guideline	1213:1223	a guideline by brewers to control palate fullness and mouthfeel	1213:1275	This model can be used as a guideline by brewers to control palate fullness and mouthfeel.
33229155	7	36	theme	composition	898:908	arg1	parameters					910:919	composition parameters	898:919	composition parameters	898:919	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	3	37	theme	systematic	260:269	arg1	correlations					271:282	systematic correlations	260:282	systematic correlations between sensory attributes and native beer compounds	260:335	However, systematic correlations between sensory attributes and native beer compounds have not been evaluated within the concentration range found in lager beer.
33229155	2	38	theme	molar	166:170	arg1	fractions					177:185	molar mass fractions	166:185	molar mass fractions	166:185	Non-volatile substances and molar mass fractions influence sensory perceptions of palate fullness and mouthfeel.
33229155	7	39	theme	total	991:995	arg1	p < 0.001					1007:1015	p < 0.001	1007:1015	p < 0.001	1007:1015	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	7	39	theme	total	991:995	arg1	nitrogen					997:1004	total nitrogen	991:1004	total nitrogen (p < 0.001)	991:1016	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	0	40	theme	fullness	31:38	arg1	modeling					12:19	Chemometric modeling	0:19	Chemometric modeling of palate fullness in lager	0:47	Chemometric modeling of palate fullness in lager beers.
33229155	4	41	theme	fullness	534:541	arg1	data					500:503	analytical data	489:503	analytical data of beer compositions, palate fullness, and mouthfeel descriptors	489:568	This article reports a chemometric analysis of 41 lager beers by evaluating analytical data of beer compositions, palate fullness, and mouthfeel descriptors.
33229155	4	42	theme	beers	469:473	arg1	analysis					448:455	a chemometric analysis	434:455	a chemometric analysis of 41 lager beers	434:473	This article reports a chemometric analysis of 41 lager beers by evaluating analytical data of beer compositions, palate fullness, and mouthfeel descriptors.
33229155	2	43	theme	fullness	227:234	arg1	perceptions					205:215	sensory perceptions	197:215	sensory perceptions of palate fullness and mouthfeel	197:248	Non-volatile substances and molar mass fractions influence sensory perceptions of palate fullness and mouthfeel.
33229155	4	44	theme	palate	527:532	arg1	fullness					534:541	palate fullness	527:541	palate fullness	527:541	This article reports a chemometric analysis of 41 lager beers by evaluating analytical data of beer compositions, palate fullness, and mouthfeel descriptors.
33229155	3	45	theme	native	315:320	arg1	compounds					327:335	native beer compounds	315:335	native beer compounds	315:335	However, systematic correlations between sensory attributes and native beer compounds have not been evaluated within the concentration range found in lager beer.
33229155	4	46	theme	lager	463:467	arg1	beers					469:473	41 lager beers	460:473	41 lager beers	460:473	This article reports a chemometric analysis of 41 lager beers by evaluating analytical data of beer compositions, palate fullness, and mouthfeel descriptors.
33229155	2	47	theme	palate	220:225	arg1	fullness					227:234	palate fullness	220:234	palate fullness	220:234	Non-volatile substances and molar mass fractions influence sensory perceptions of palate fullness and mouthfeel.
33229155	1	48	theme	key	98:100	arg1	fullness					63:70	Palate fullness	56:70	Palate fullness	56:70	Palate fullness and mouthfeel of beer are key attributes of sensory beer quality.
33229155	1	48	theme	key	98:100	arg1	attributes					102:111	key attributes	98:111	key attributes of sensory beer quality	98:135	Palate fullness and mouthfeel of beer are key attributes of sensory beer quality.
33229155	1	48	theme	key	98:100	arg1	mouthfeel					76:84	mouthfeel	76:84	mouthfeel	76:84	Palate fullness and mouthfeel of beer are key attributes of sensory beer quality.
33229155	3	49	theme	beer	322:325	arg1	compounds					327:335	native beer compounds	315:335	native beer compounds	315:335	However, systematic correlations between sensory attributes and native beer compounds have not been evaluated within the concentration range found in lager beer.
33229155	7	50	theme	palate	844:849	arg1	fullness					851:858	palate fullness	844:858	palate fullness	844:858	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	8	51	theme	palate	1136:1141	arg1	intensity					1152:1160	the palate fullness intensity	1132:1160	the palate fullness intensity	1132:1160	Thus, a model was built using partial least square regression (PLS) analysis to predict the palate fullness intensity in beers (R2C = 0.7993).
33229155	2	52	theme	sensory	197:203	arg1	perceptions					205:215	sensory perceptions	197:215	sensory perceptions of palate fullness and mouthfeel	197:248	Non-volatile substances and molar mass fractions influence sensory perceptions of palate fullness and mouthfeel.
33229155	3	53	located	found	392:396	arg2	range					386:390	the concentration range	368:390	the concentration range found in lager beer	368:410	However, systematic correlations between sensory attributes and native beer compounds have not been evaluated within the concentration range found in lager beer.
33229155	3	53	located	found	392:396	arg1	beer					407:410	lager beer	401:410	lager beer	401:410	However, systematic correlations between sensory attributes and native beer compounds have not been evaluated within the concentration range found in lager beer.
33229155	1	54	theme	sensory	116:122	arg1	quality					129:135	sensory beer quality	116:135	sensory beer quality	116:135	Palate fullness and mouthfeel of beer are key attributes of sensory beer quality.
33229155	4	55	theme	chemometric	436:446	arg1	analysis					448:455	a chemometric analysis	434:455	a chemometric analysis of 41 lager beers	434:473	This article reports a chemometric analysis of 41 lager beers by evaluating analytical data of beer compositions, palate fullness, and mouthfeel descriptors.
33229155	1	56	theme	beer	124:127	arg1	quality					129:135	sensory beer quality	116:135	sensory beer quality	116:135	Palate fullness and mouthfeel of beer are key attributes of sensory beer quality.
33229155	6	57	theme	Screened	672:679	arg1	beers					681:685	Screened beers	672:685	Screened beers	672:685	Screened beers were clustered into groups differing significantly in palate fullness intensity and macromolecular distribution.
33229155	7	58	theme	Significant	800:810	arg1	correlations					812:823	Significant correlations	800:823	Significant correlations	800:823	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
33229155	4	59	theme	beer	508:511	arg1	compositions					513:524	beer compositions	508:524	beer compositions	508:524	This article reports a chemometric analysis of 41 lager beers by evaluating analytical data of beer compositions, palate fullness, and mouthfeel descriptors.
33229155	2	60	theme	mouthfeel	240:248	arg1	perceptions					205:215	sensory perceptions	197:215	sensory perceptions of palate fullness and mouthfeel	197:248	Non-volatile substances and molar mass fractions influence sensory perceptions of palate fullness and mouthfeel.
33229155	6	61	theme	macromolecular	771:784	arg1	distribution					786:797	macromolecular distribution	771:797	macromolecular distribution	771:797	Screened beers were clustered into groups differing significantly in palate fullness intensity and macromolecular distribution.
33229155	1	62	theme	quality	129:135	arg1	fullness					63:70	Palate fullness	56:70	Palate fullness	56:70	Palate fullness and mouthfeel of beer are key attributes of sensory beer quality.
33229155	1	62	theme	quality	129:135	arg1	attributes					102:111	key attributes	98:111	key attributes of sensory beer quality	98:135	Palate fullness and mouthfeel of beer are key attributes of sensory beer quality.
33229155	1	62	theme	quality	129:135	arg1	mouthfeel					76:84	mouthfeel	76:84	mouthfeel	76:84	Palate fullness and mouthfeel of beer are key attributes of sensory beer quality.
33229155	7	63	theme	macromolecular	962:975	arg1	distribution					977:988	macromolecular distribution	962:988	macromolecular distribution	962:988	Significant correlations were found between palate fullness and macromolecular fractions and beer composition parameters: original gravity, viscosity, indices of macromolecular distribution, total nitrogen (p < 0.001), and β-glucan (p < 0.01).
32910639	4	0	theme	traditional	760:770	arg1	blends					783:788	the traditional biopolymer blends	756:788	the traditional biopolymer blends	756:788	In contrast to the traditional biopolymer blends, the alternating CNC-CNF stacked films possess many-fold enhancement in both mechanical strength and toughness with their vivid structural colors highly preserved.
32910639	6	1	theme	facile	1315:1320	arg1	strategy					1333:1340	a facile sequential strategy	1313:1340	a facile sequential strategy	1313:1340	Such organized cellulose-cellulose biocomposites with alternating helicoidal-random phases fabricated by a facile sequential strategy may facilitate the development of sustainably sourced, damage-tolerant, and photonic films for bioenabled display technologies, security indicators, soft robotics, camouflages, and pressure sensors.
32910639	3	2	with	biocomposites	572:584	arg1	phase					680:684	a random cellulose nanofiber (CNF) phase	645:684	a random cellulose nanofiber (CNF) phase	645:684	Herein, we report a design of robust laminated biocomposites with an alternatively stacked chiral nematic CNC phase and a random cellulose nanofiber (CNF) phase via a hydrogen-bonding-assisted layer-by-layer method.
32910639	3	2	with	biocomposites	572:584	arg1	phase					635:639	an alternatively stacked chiral nematic CNC phase	591:639	an alternatively stacked chiral nematic CNC phase	591:639	Herein, we report a design of robust laminated biocomposites with an alternatively stacked chiral nematic CNC phase and a random cellulose nanofiber (CNF) phase via a hydrogen-bonding-assisted layer-by-layer method.
32910639	5	3	theme	biocomposites	1160:1172	arg1	performance					1138:1148	the mechanical performance	1123:1148	the mechanical performance of robust biocomposites with brilliant iridescent colors	1123:1205	We suggest that the enriched hydrogen bonding and partial limited entanglements at the interfaces between the helicoidal and random phases are responsible for enhancing the mechanical performance of robust biocomposites with brilliant iridescent colors.
32910639	5	4	theme	hydrogen	983:990	arg1	responsible					1097:1107	responsible	1097:1107	responsible	1097:1107	We suggest that the enriched hydrogen bonding and partial limited entanglements at the interfaces between the helicoidal and random phases are responsible for enhancing the mechanical performance of robust biocomposites with brilliant iridescent colors.
32910639	5	4	theme	hydrogen	983:990	arg1	bonding					992:998	the enriched hydrogen bonding	970:998	the enriched hydrogen bonding	970:998	We suggest that the enriched hydrogen bonding and partial limited entanglements at the interfaces between the helicoidal and random phases are responsible for enhancing the mechanical performance of robust biocomposites with brilliant iridescent colors.
32910639	2	5	theme	mechanical	399:408	arg1	performance					410:420	weak mechanical performance	394:420	weak mechanical performance due to poor load transfer between needle-like nanocrystals	394:479	Traditional cellulose nanocrystal (CNC) blend nanocomposite films typically lose their coloration and display weak mechanical performance due to poor load transfer between needle-like nanocrystals and the collapse of a twisted organization.
32910639	5	6	theme	enriched	974:981	arg1	responsible					1097:1107	responsible	1097:1107	responsible	1097:1107	We suggest that the enriched hydrogen bonding and partial limited entanglements at the interfaces between the helicoidal and random phases are responsible for enhancing the mechanical performance of robust biocomposites with brilliant iridescent colors.
32910639	5	6	theme	enriched	974:981	arg1	bonding					992:998	the enriched hydrogen bonding	970:998	the enriched hydrogen bonding	970:998	We suggest that the enriched hydrogen bonding and partial limited entanglements at the interfaces between the helicoidal and random phases are responsible for enhancing the mechanical performance of robust biocomposites with brilliant iridescent colors.
32910639	6	7	theme	organized	1213:1221	arg1	biocomposites					1243:1255	Such organized cellulose-cellulose biocomposites	1208:1255	Such organized cellulose-cellulose biocomposites with alternating helicoidal-random phases fabricated by a facile sequential strategy	1208:1340	Such organized cellulose-cellulose biocomposites with alternating helicoidal-random phases fabricated by a facile sequential strategy may facilitate the development of sustainably sourced, damage-tolerant, and photonic films for bioenabled display technologies, security indicators, soft robotics, camouflages, and pressure sensors.
32910639	6	8	theme	display	1448:1454	arg1	technologies					1456:1467	bioenabled display technologies	1437:1467	bioenabled display technologies	1437:1467	Such organized cellulose-cellulose biocomposites with alternating helicoidal-random phases fabricated by a facile sequential strategy may facilitate the development of sustainably sourced, damage-tolerant, and photonic films for bioenabled display technologies, security indicators, soft robotics, camouflages, and pressure sensors.
32910639	4	9	from	enhancement	847:857	arg1	strength					878:885	mechanical strength	867:885	mechanical strength	867:885	In contrast to the traditional biopolymer blends, the alternating CNC-CNF stacked films possess many-fold enhancement in both mechanical strength and toughness with their vivid structural colors highly preserved.
32910639	4	9	from	enhancement	847:857	arg1	toughness					891:899	toughness	891:899	toughness	891:899	In contrast to the traditional biopolymer blends, the alternating CNC-CNF stacked films possess many-fold enhancement in both mechanical strength and toughness with their vivid structural colors highly preserved.
32910639	2	10	theme	nanocomposite	330:342	arg1	films					344:348	Traditional cellulose nanocrystal (CNC) blend nanocomposite films	284:348	Traditional cellulose nanocrystal (CNC) blend nanocomposite films	284:348	Traditional cellulose nanocrystal (CNC) blend nanocomposite films typically lose their coloration and display weak mechanical performance due to poor load transfer between needle-like nanocrystals and the collapse of a twisted organization.
32910639	3	11	theme	nematic	623:629	arg1	phase					635:639	an alternatively stacked chiral nematic CNC phase	591:639	an alternatively stacked chiral nematic CNC phase	591:639	Herein, we report a design of robust laminated biocomposites with an alternatively stacked chiral nematic CNC phase and a random cellulose nanofiber (CNF) phase via a hydrogen-bonding-assisted layer-by-layer method.
32910639	4	12	theme	CNC-CNF	807:813	arg1	films					823:827	the alternating CNC-CNF stacked films	791:827	the alternating CNC-CNF stacked films	791:827	In contrast to the traditional biopolymer blends, the alternating CNC-CNF stacked films possess many-fold enhancement in both mechanical strength and toughness with their vivid structural colors highly preserved.
32910639	3	13	theme	stacked	608:614	arg1	phase					635:639	an alternatively stacked chiral nematic CNC phase	591:639	an alternatively stacked chiral nematic CNC phase	591:639	Herein, we report a design of robust laminated biocomposites with an alternatively stacked chiral nematic CNC phase and a random cellulose nanofiber (CNF) phase via a hydrogen-bonding-assisted layer-by-layer method.
32910639	6	14	theme	bioenabled	1437:1446	arg1	technologies					1456:1467	bioenabled display technologies	1437:1467	bioenabled display technologies	1437:1467	Such organized cellulose-cellulose biocomposites with alternating helicoidal-random phases fabricated by a facile sequential strategy may facilitate the development of sustainably sourced, damage-tolerant, and photonic films for bioenabled display technologies, security indicators, soft robotics, camouflages, and pressure sensors.
32910639	2	15	theme	blend	324:328	arg1	films					344:348	Traditional cellulose nanocrystal (CNC) blend nanocomposite films	284:348	Traditional cellulose nanocrystal (CNC) blend nanocomposite films	284:348	Traditional cellulose nanocrystal (CNC) blend nanocomposite films typically lose their coloration and display weak mechanical performance due to poor load transfer between needle-like nanocrystals and the collapse of a twisted organization.
32910639	5	16	with	biocomposites	1160:1172	arg1	colors					1200:1205	brilliant iridescent colors	1179:1205	brilliant iridescent colors	1179:1205	We suggest that the enriched hydrogen bonding and partial limited entanglements at the interfaces between the helicoidal and random phases are responsible for enhancing the mechanical performance of robust biocomposites with brilliant iridescent colors.
32910639	4	17	dep	blends	783:788	arg1	contrast					744:751	contrast	744:751	contrast	744:751	In contrast to the traditional biopolymer blends, the alternating CNC-CNF stacked films possess many-fold enhancement in both mechanical strength and toughness with their vivid structural colors highly preserved.
32910639	6	18	theme	pressure	1523:1530	arg1	sensors					1532:1538	pressure sensors	1523:1538	pressure sensors	1523:1538	Such organized cellulose-cellulose biocomposites with alternating helicoidal-random phases fabricated by a facile sequential strategy may facilitate the development of sustainably sourced, damage-tolerant, and photonic films for bioenabled display technologies, security indicators, soft robotics, camouflages, and pressure sensors.
32910639	1	19	theme	vivid	219:223	arg1	iridescence					233:243	their vivid optical iridescence	213:243	their vivid optical iridescence	213:243	Attaining high mechanical strength and flexibility for chiral nematic biopolymer composites without compromising their vivid optical iridescence is an intriguing but challenging task.
32910639	6	20	theme	helicoidal-random	1274:1290	arg1	phases					1292:1297	alternating helicoidal-random phases	1262:1297	alternating helicoidal-random phases fabricated by a facile sequential strategy	1262:1340	Such organized cellulose-cellulose biocomposites with alternating helicoidal-random phases fabricated by a facile sequential strategy may facilitate the development of sustainably sourced, damage-tolerant, and photonic films for bioenabled display technologies, security indicators, soft robotics, camouflages, and pressure sensors.
32910639	6	21	with	biocomposites	1243:1255	arg1	phases					1292:1297	alternating helicoidal-random phases	1262:1297	alternating helicoidal-random phases fabricated by a facile sequential strategy	1262:1340	Such organized cellulose-cellulose biocomposites with alternating helicoidal-random phases fabricated by a facile sequential strategy may facilitate the development of sustainably sourced, damage-tolerant, and photonic films for bioenabled display technologies, security indicators, soft robotics, camouflages, and pressure sensors.
32910639	4	22	theme	structural	918:927	arg1	colors					929:934	their vivid structural colors	906:934	their vivid structural colors highly preserved	906:951	In contrast to the traditional biopolymer blends, the alternating CNC-CNF stacked films possess many-fold enhancement in both mechanical strength and toughness with their vivid structural colors highly preserved.
32910639	5	23	theme	random	1079:1084	arg1	phases					1086:1091	the helicoidal and random phases	1060:1091	the helicoidal and random phases	1060:1091	We suggest that the enriched hydrogen bonding and partial limited entanglements at the interfaces between the helicoidal and random phases are responsible for enhancing the mechanical performance of robust biocomposites with brilliant iridescent colors.
32910639	6	24	theme	Such	1208:1211	arg1	biocomposites					1243:1255	Such organized cellulose-cellulose biocomposites	1208:1255	Such organized cellulose-cellulose biocomposites with alternating helicoidal-random phases fabricated by a facile sequential strategy	1208:1340	Such organized cellulose-cellulose biocomposites with alternating helicoidal-random phases fabricated by a facile sequential strategy may facilitate the development of sustainably sourced, damage-tolerant, and photonic films for bioenabled display technologies, security indicators, soft robotics, camouflages, and pressure sensors.
32910639	2	25	theme	twisted	503:509	arg1	organization					511:522	a twisted organization	501:522	a twisted organization	501:522	Traditional cellulose nanocrystal (CNC) blend nanocomposite films typically lose their coloration and display weak mechanical performance due to poor load transfer between needle-like nanocrystals and the collapse of a twisted organization.
32910639	3	26	theme	biocomposites	572:584	arg1	design					545:550	a design	543:550	a design of robust laminated biocomposites with an alternatively stacked chiral nematic CNC phase and a random cellulose nanofiber (CNF) phase	543:684	Herein, we report a design of robust laminated biocomposites with an alternatively stacked chiral nematic CNC phase and a random cellulose nanofiber (CNF) phase via a hydrogen-bonding-assisted layer-by-layer method.
32910639	4	27	theme	preserved	943:951	arg1	colors					929:934	their vivid structural colors	906:934	their vivid structural colors highly preserved	906:951	In contrast to the traditional biopolymer blends, the alternating CNC-CNF stacked films possess many-fold enhancement in both mechanical strength and toughness with their vivid structural colors highly preserved.
32910639	3	28	theme	CNC	631:633	arg1	phase					635:639	an alternatively stacked chiral nematic CNC phase	591:639	an alternatively stacked chiral nematic CNC phase	591:639	Herein, we report a design of robust laminated biocomposites with an alternatively stacked chiral nematic CNC phase and a random cellulose nanofiber (CNF) phase via a hydrogen-bonding-assisted layer-by-layer method.
32910639	2	29	theme	load	434:437	arg1	transfer					439:446	poor load transfer	429:446	poor load transfer between needle-like nanocrystals	429:479	Traditional cellulose nanocrystal (CNC) blend nanocomposite films typically lose their coloration and display weak mechanical performance due to poor load transfer between needle-like nanocrystals and the collapse of a twisted organization.
32910639	1	30	theme	high	110:113	arg1	strength					126:133	high mechanical strength	110:133	high mechanical strength	110:133	Attaining high mechanical strength and flexibility for chiral nematic biopolymer composites without compromising their vivid optical iridescence is an intriguing but challenging task.
32910639	4	31	theme	many-fold	837:845	arg1	enhancement					847:857	many-fold enhancement	837:857	many-fold enhancement in both mechanical strength and toughness with their vivid structural colors highly preserved	837:951	In contrast to the traditional biopolymer blends, the alternating CNC-CNF stacked films possess many-fold enhancement in both mechanical strength and toughness with their vivid structural colors highly preserved.
32910639	1	32	theme	optical	225:231	arg1	iridescence					233:243	their vivid optical iridescence	213:243	their vivid optical iridescence	213:243	Attaining high mechanical strength and flexibility for chiral nematic biopolymer composites without compromising their vivid optical iridescence is an intriguing but challenging task.
32910639	0	33	theme	Nanocrystals	24:35	arg1	Stacking					12:19	Stacking	12:19	Stacking of Nanocrystals and Nanofibers	12:50	Alternating Stacking of Nanocrystals and Nanofibers into Ultrastrong Chiral Biocomposite Laminates.
32910639	3	34	theme	nanofiber	664:672	arg1	phase					680:684	a random cellulose nanofiber (CNF) phase	645:684	a random cellulose nanofiber (CNF) phase	645:684	Herein, we report a design of robust laminated biocomposites with an alternatively stacked chiral nematic CNC phase and a random cellulose nanofiber (CNF) phase via a hydrogen-bonding-assisted layer-by-layer method.
32910639	6	35	theme	sourced	1388:1394	arg1	development					1361:1371	the development	1357:1371	the development of sustainably sourced, damage-tolerant, and photonic films for bioenabled display technologies, security indicators, soft robotics, camouflages, and pressure sensors	1357:1538	Such organized cellulose-cellulose biocomposites with alternating helicoidal-random phases fabricated by a facile sequential strategy may facilitate the development of sustainably sourced, damage-tolerant, and photonic films for bioenabled display technologies, security indicators, soft robotics, camouflages, and pressure sensors.
32910639	1	36	theme	mechanical	115:124	arg1	strength					126:133	high mechanical strength	110:133	high mechanical strength	110:133	Attaining high mechanical strength and flexibility for chiral nematic biopolymer composites without compromising their vivid optical iridescence is an intriguing but challenging task.
32910639	4	37	theme	alternating	795:805	arg1	films					823:827	the alternating CNC-CNF stacked films	791:827	the alternating CNC-CNF stacked films	791:827	In contrast to the traditional biopolymer blends, the alternating CNC-CNF stacked films possess many-fold enhancement in both mechanical strength and toughness with their vivid structural colors highly preserved.
32910639	5	38	theme	brilliant	1179:1187	arg1	colors					1200:1205	brilliant iridescent colors	1179:1205	brilliant iridescent colors	1179:1205	We suggest that the enriched hydrogen bonding and partial limited entanglements at the interfaces between the helicoidal and random phases are responsible for enhancing the mechanical performance of robust biocomposites with brilliant iridescent colors.
32910639	3	39	theme	robust	555:560	arg1	biocomposites					572:584	robust laminated biocomposites	555:584	robust laminated biocomposites with an alternatively stacked chiral nematic CNC phase and a random cellulose nanofiber (CNF) phase	555:684	Herein, we report a design of robust laminated biocomposites with an alternatively stacked chiral nematic CNC phase and a random cellulose nanofiber (CNF) phase via a hydrogen-bonding-assisted layer-by-layer method.
32910639	5	40	theme	mechanical	1127:1136	arg1	performance					1138:1148	the mechanical performance	1123:1148	the mechanical performance of robust biocomposites with brilliant iridescent colors	1123:1205	We suggest that the enriched hydrogen bonding and partial limited entanglements at the interfaces between the helicoidal and random phases are responsible for enhancing the mechanical performance of robust biocomposites with brilliant iridescent colors.
32910639	3	41	theme	laminated	562:570	arg1	biocomposites					572:584	robust laminated biocomposites	555:584	robust laminated biocomposites with an alternatively stacked chiral nematic CNC phase and a random cellulose nanofiber (CNF) phase	555:684	Herein, we report a design of robust laminated biocomposites with an alternatively stacked chiral nematic CNC phase and a random cellulose nanofiber (CNF) phase via a hydrogen-bonding-assisted layer-by-layer method.
32910639	0	42	theme	Nanofibers	41:50	arg1	Stacking					12:19	Stacking	12:19	Stacking of Nanocrystals and Nanofibers	12:50	Alternating Stacking of Nanocrystals and Nanofibers into Ultrastrong Chiral Biocomposite Laminates.
32910639	5	43	theme	partial	1004:1010	arg1	entanglements					1020:1032	partial limited entanglements	1004:1032	partial limited entanglements at the interfaces between the helicoidal and random phases	1004:1091	We suggest that the enriched hydrogen bonding and partial limited entanglements at the interfaces between the helicoidal and random phases are responsible for enhancing the mechanical performance of robust biocomposites with brilliant iridescent colors.
32910639	3	44	theme	hydrogen-bonding-assisted	692:716	arg1	method					733:738	a hydrogen-bonding-assisted layer-by-layer method	690:738	a hydrogen-bonding-assisted layer-by-layer method	690:738	Herein, we report a design of robust laminated biocomposites with an alternatively stacked chiral nematic CNC phase and a random cellulose nanofiber (CNF) phase via a hydrogen-bonding-assisted layer-by-layer method.
32910639	3	45	theme	random	647:652	arg1	CNF					675:677	CNF	675:677	CNF	675:677	Herein, we report a design of robust laminated biocomposites with an alternatively stacked chiral nematic CNC phase and a random cellulose nanofiber (CNF) phase via a hydrogen-bonding-assisted layer-by-layer method.
32910639	3	45	theme	random	647:652	arg1	nanofiber					664:672	a random cellulose nanofiber	645:672	a random cellulose nanofiber (CNF) phase	645:684	Herein, we report a design of robust laminated biocomposites with an alternatively stacked chiral nematic CNC phase and a random cellulose nanofiber (CNF) phase via a hydrogen-bonding-assisted layer-by-layer method.
32910639	2	46	theme	poor	429:432	arg1	transfer					439:446	poor load transfer	429:446	poor load transfer between needle-like nanocrystals	429:479	Traditional cellulose nanocrystal (CNC) blend nanocomposite films typically lose their coloration and display weak mechanical performance due to poor load transfer between needle-like nanocrystals and the collapse of a twisted organization.
32910639	6	47	theme	films	1427:1431	arg1	development					1361:1371	the development	1357:1371	the development of sustainably sourced, damage-tolerant, and photonic films for bioenabled display technologies, security indicators, soft robotics, camouflages, and pressure sensors	1357:1538	Such organized cellulose-cellulose biocomposites with alternating helicoidal-random phases fabricated by a facile sequential strategy may facilitate the development of sustainably sourced, damage-tolerant, and photonic films for bioenabled display technologies, security indicators, soft robotics, camouflages, and pressure sensors.
32910639	6	48	theme	alternating	1262:1272	arg1	phases					1292:1297	alternating helicoidal-random phases	1262:1297	alternating helicoidal-random phases fabricated by a facile sequential strategy	1262:1340	Such organized cellulose-cellulose biocomposites with alternating helicoidal-random phases fabricated by a facile sequential strategy may facilitate the development of sustainably sourced, damage-tolerant, and photonic films for bioenabled display technologies, security indicators, soft robotics, camouflages, and pressure sensors.
32910639	3	49	theme	cellulose	654:662	arg1	CNF					675:677	CNF	675:677	CNF	675:677	Herein, we report a design of robust laminated biocomposites with an alternatively stacked chiral nematic CNC phase and a random cellulose nanofiber (CNF) phase via a hydrogen-bonding-assisted layer-by-layer method.
32910639	3	49	theme	cellulose	654:662	arg1	nanofiber					664:672	a random cellulose nanofiber	645:672	a random cellulose nanofiber (CNF) phase	645:684	Herein, we report a design of robust laminated biocomposites with an alternatively stacked chiral nematic CNC phase and a random cellulose nanofiber (CNF) phase via a hydrogen-bonding-assisted layer-by-layer method.
32910639	1	50	theme	intriguing	251:260	arg1	task					278:281	an intriguing but challenging task	248:281	an intriguing but challenging task	248:281	Attaining high mechanical strength and flexibility for chiral nematic biopolymer composites without compromising their vivid optical iridescence is an intriguing but challenging task.
32910639	0	51	theme	Ultrastrong	57:67	arg1	Laminates					89:97	Ultrastrong Chiral Biocomposite Laminates	57:97	Ultrastrong Chiral Biocomposite Laminates	57:97	Alternating Stacking of Nanocrystals and Nanofibers into Ultrastrong Chiral Biocomposite Laminates.
32910639	6	52	theme	soft	1491:1494	arg1	robotics					1496:1503	soft robotics	1491:1503	soft robotics	1491:1503	Such organized cellulose-cellulose biocomposites with alternating helicoidal-random phases fabricated by a facile sequential strategy may facilitate the development of sustainably sourced, damage-tolerant, and photonic films for bioenabled display technologies, security indicators, soft robotics, camouflages, and pressure sensors.
32910639	4	53	with	enhancement	847:857	arg1	colors					929:934	their vivid structural colors	906:934	their vivid structural colors highly preserved	906:951	In contrast to the traditional biopolymer blends, the alternating CNC-CNF stacked films possess many-fold enhancement in both mechanical strength and toughness with their vivid structural colors highly preserved.
32910639	4	54	theme	vivid	912:916	arg1	colors					929:934	their vivid structural colors	906:934	their vivid structural colors highly preserved	906:951	In contrast to the traditional biopolymer blends, the alternating CNC-CNF stacked films possess many-fold enhancement in both mechanical strength and toughness with their vivid structural colors highly preserved.
32910639	6	55	theme	cellulose-cellulose	1223:1241	arg1	biocomposites					1243:1255	Such organized cellulose-cellulose biocomposites	1208:1255	Such organized cellulose-cellulose biocomposites with alternating helicoidal-random phases fabricated by a facile sequential strategy	1208:1340	Such organized cellulose-cellulose biocomposites with alternating helicoidal-random phases fabricated by a facile sequential strategy may facilitate the development of sustainably sourced, damage-tolerant, and photonic films for bioenabled display technologies, security indicators, soft robotics, camouflages, and pressure sensors.
32910639	2	56	theme	weak	394:397	arg1	performance					410:420	weak mechanical performance	394:420	weak mechanical performance due to poor load transfer between needle-like nanocrystals	394:479	Traditional cellulose nanocrystal (CNC) blend nanocomposite films typically lose their coloration and display weak mechanical performance due to poor load transfer between needle-like nanocrystals and the collapse of a twisted organization.
32910639	2	57	theme	organization	511:522	arg1	performance					410:420	weak mechanical performance	394:420	weak mechanical performance due to poor load transfer between needle-like nanocrystals	394:479	Traditional cellulose nanocrystal (CNC) blend nanocomposite films typically lose their coloration and display weak mechanical performance due to poor load transfer between needle-like nanocrystals and the collapse of a twisted organization.
32910639	2	57	theme	organization	511:522	arg1	collapse					489:496	the collapse	485:496	the collapse of a twisted organization	485:522	Traditional cellulose nanocrystal (CNC) blend nanocomposite films typically lose their coloration and display weak mechanical performance due to poor load transfer between needle-like nanocrystals and the collapse of a twisted organization.
32910639	4	58	contain	possess	829:835	arg1	films					823:827	the alternating CNC-CNF stacked films	791:827	the alternating CNC-CNF stacked films	791:827	In contrast to the traditional biopolymer blends, the alternating CNC-CNF stacked films possess many-fold enhancement in both mechanical strength and toughness with their vivid structural colors highly preserved.
32910639	4	58	contain	possess	829:835	arg2	enhancement					847:857	many-fold enhancement	837:857	many-fold enhancement in both mechanical strength and toughness with their vivid structural colors highly preserved	837:951	In contrast to the traditional biopolymer blends, the alternating CNC-CNF stacked films possess many-fold enhancement in both mechanical strength and toughness with their vivid structural colors highly preserved.
32910639	5	59	theme	iridescent	1189:1198	arg1	colors					1200:1205	brilliant iridescent colors	1179:1205	brilliant iridescent colors	1179:1205	We suggest that the enriched hydrogen bonding and partial limited entanglements at the interfaces between the helicoidal and random phases are responsible for enhancing the mechanical performance of robust biocomposites with brilliant iridescent colors.
32910639	4	60	theme	biopolymer	772:781	arg1	blends					783:788	the traditional biopolymer blends	756:788	the traditional biopolymer blends	756:788	In contrast to the traditional biopolymer blends, the alternating CNC-CNF stacked films possess many-fold enhancement in both mechanical strength and toughness with their vivid structural colors highly preserved.
32910639	5	61	from	interfaces	1041:1050	arg1	responsible					1097:1107	responsible	1097:1107	responsible	1097:1107	We suggest that the enriched hydrogen bonding and partial limited entanglements at the interfaces between the helicoidal and random phases are responsible for enhancing the mechanical performance of robust biocomposites with brilliant iridescent colors.
32910639	5	61	from	interfaces	1041:1050	arg1	bonding					992:998	the enriched hydrogen bonding	970:998	the enriched hydrogen bonding	970:998	We suggest that the enriched hydrogen bonding and partial limited entanglements at the interfaces between the helicoidal and random phases are responsible for enhancing the mechanical performance of robust biocomposites with brilliant iridescent colors.
32910639	5	61	from	interfaces	1041:1050	arg1	entanglements					1020:1032	partial limited entanglements	1004:1032	partial limited entanglements at the interfaces between the helicoidal and random phases	1004:1091	We suggest that the enriched hydrogen bonding and partial limited entanglements at the interfaces between the helicoidal and random phases are responsible for enhancing the mechanical performance of robust biocomposites with brilliant iridescent colors.
32910639	5	62	theme	robust	1153:1158	arg1	biocomposites					1160:1172	robust biocomposites	1153:1172	robust biocomposites with brilliant iridescent colors	1153:1205	We suggest that the enriched hydrogen bonding and partial limited entanglements at the interfaces between the helicoidal and random phases are responsible for enhancing the mechanical performance of robust biocomposites with brilliant iridescent colors.
32910639	4	63	theme	mechanical	867:876	arg1	strength					878:885	mechanical strength	867:885	mechanical strength	867:885	In contrast to the traditional biopolymer blends, the alternating CNC-CNF stacked films possess many-fold enhancement in both mechanical strength and toughness with their vivid structural colors highly preserved.
32910639	6	64	theme	damage-tolerant	1397:1411	arg1	development					1361:1371	the development	1357:1371	the development of sustainably sourced, damage-tolerant, and photonic films for bioenabled display technologies, security indicators, soft robotics, camouflages, and pressure sensors	1357:1538	Such organized cellulose-cellulose biocomposites with alternating helicoidal-random phases fabricated by a facile sequential strategy may facilitate the development of sustainably sourced, damage-tolerant, and photonic films for bioenabled display technologies, security indicators, soft robotics, camouflages, and pressure sensors.
32910639	5	65	theme	limited	1012:1018	arg1	entanglements					1020:1032	partial limited entanglements	1004:1032	partial limited entanglements at the interfaces between the helicoidal and random phases	1004:1091	We suggest that the enriched hydrogen bonding and partial limited entanglements at the interfaces between the helicoidal and random phases are responsible for enhancing the mechanical performance of robust biocomposites with brilliant iridescent colors.
32910639	3	66	theme	chiral	616:621	arg1	phase					635:639	an alternatively stacked chiral nematic CNC phase	591:639	an alternatively stacked chiral nematic CNC phase	591:639	Herein, we report a design of robust laminated biocomposites with an alternatively stacked chiral nematic CNC phase and a random cellulose nanofiber (CNF) phase via a hydrogen-bonding-assisted layer-by-layer method.
32910639	6	67	theme	photonic	1418:1425	arg1	films					1427:1431	photonic films	1418:1431	photonic films	1418:1431	Such organized cellulose-cellulose biocomposites with alternating helicoidal-random phases fabricated by a facile sequential strategy may facilitate the development of sustainably sourced, damage-tolerant, and photonic films for bioenabled display technologies, security indicators, soft robotics, camouflages, and pressure sensors.
32910639	6	68	theme	security	1470:1477	arg1	indicators					1479:1488	security indicators	1470:1488	security indicators	1470:1488	Such organized cellulose-cellulose biocomposites with alternating helicoidal-random phases fabricated by a facile sequential strategy may facilitate the development of sustainably sourced, damage-tolerant, and photonic films for bioenabled display technologies, security indicators, soft robotics, camouflages, and pressure sensors.
32910639	3	69	theme	layer-by-layer	718:731	arg1	method					733:738	a hydrogen-bonding-assisted layer-by-layer method	690:738	a hydrogen-bonding-assisted layer-by-layer method	690:738	Herein, we report a design of robust laminated biocomposites with an alternatively stacked chiral nematic CNC phase and a random cellulose nanofiber (CNF) phase via a hydrogen-bonding-assisted layer-by-layer method.
32910639	2	70	theme	nanocrystal	306:316	arg1	films					344:348	Traditional cellulose nanocrystal (CNC) blend nanocomposite films	284:348	Traditional cellulose nanocrystal (CNC) blend nanocomposite films	284:348	Traditional cellulose nanocrystal (CNC) blend nanocomposite films typically lose their coloration and display weak mechanical performance due to poor load transfer between needle-like nanocrystals and the collapse of a twisted organization.
32910639	4	71	theme	stacked	815:821	arg1	films					823:827	the alternating CNC-CNF stacked films	791:827	the alternating CNC-CNF stacked films	791:827	In contrast to the traditional biopolymer blends, the alternating CNC-CNF stacked films possess many-fold enhancement in both mechanical strength and toughness with their vivid structural colors highly preserved.
32910639	1	72	theme	chiral	155:160	arg1	composites					181:190	chiral nematic biopolymer composites	155:190	chiral nematic biopolymer composites	155:190	Attaining high mechanical strength and flexibility for chiral nematic biopolymer composites without compromising their vivid optical iridescence is an intriguing but challenging task.
32910639	1	73	theme	challenging	266:276	arg1	task					278:281	an intriguing but challenging task	248:281	an intriguing but challenging task	248:281	Attaining high mechanical strength and flexibility for chiral nematic biopolymer composites without compromising their vivid optical iridescence is an intriguing but challenging task.
32910639	0	74	theme	Biocomposite	76:87	arg1	Laminates					89:97	Ultrastrong Chiral Biocomposite Laminates	57:97	Ultrastrong Chiral Biocomposite Laminates	57:97	Alternating Stacking of Nanocrystals and Nanofibers into Ultrastrong Chiral Biocomposite Laminates.
32910639	2	75	theme	needle-like	456:466	arg1	nanocrystals					468:479	needle-like nanocrystals	456:479	needle-like nanocrystals	456:479	Traditional cellulose nanocrystal (CNC) blend nanocomposite films typically lose their coloration and display weak mechanical performance due to poor load transfer between needle-like nanocrystals and the collapse of a twisted organization.
32910639	2	76	theme	cellulose	296:304	arg1	CNC					319:321	CNC	319:321	CNC	319:321	Traditional cellulose nanocrystal (CNC) blend nanocomposite films typically lose their coloration and display weak mechanical performance due to poor load transfer between needle-like nanocrystals and the collapse of a twisted organization.
32910639	2	76	theme	cellulose	296:304	arg1	nanocrystal					306:316	Traditional cellulose nanocrystal	284:316	Traditional cellulose nanocrystal (CNC) blend nanocomposite films	284:348	Traditional cellulose nanocrystal (CNC) blend nanocomposite films typically lose their coloration and display weak mechanical performance due to poor load transfer between needle-like nanocrystals and the collapse of a twisted organization.
32910639	6	77	theme	sequential	1322:1331	arg1	strategy					1333:1340	a facile sequential strategy	1313:1340	a facile sequential strategy	1313:1340	Such organized cellulose-cellulose biocomposites with alternating helicoidal-random phases fabricated by a facile sequential strategy may facilitate the development of sustainably sourced, damage-tolerant, and photonic films for bioenabled display technologies, security indicators, soft robotics, camouflages, and pressure sensors.
32910639	1	78	theme	nematic	162:168	arg1	composites					181:190	chiral nematic biopolymer composites	155:190	chiral nematic biopolymer composites	155:190	Attaining high mechanical strength and flexibility for chiral nematic biopolymer composites without compromising their vivid optical iridescence is an intriguing but challenging task.
32910639	0	79	theme	Chiral	69:74	arg1	Laminates					89:97	Ultrastrong Chiral Biocomposite Laminates	57:97	Ultrastrong Chiral Biocomposite Laminates	57:97	Alternating Stacking of Nanocrystals and Nanofibers into Ultrastrong Chiral Biocomposite Laminates.
32910639	5	80	theme	helicoidal	1064:1073	arg1	phases					1086:1091	the helicoidal and random phases	1060:1091	the helicoidal and random phases	1060:1091	We suggest that the enriched hydrogen bonding and partial limited entanglements at the interfaces between the helicoidal and random phases are responsible for enhancing the mechanical performance of robust biocomposites with brilliant iridescent colors.
32910639	2	81	theme	Traditional	284:294	arg1	CNC					319:321	CNC	319:321	CNC	319:321	Traditional cellulose nanocrystal (CNC) blend nanocomposite films typically lose their coloration and display weak mechanical performance due to poor load transfer between needle-like nanocrystals and the collapse of a twisted organization.
32910639	2	81	theme	Traditional	284:294	arg1	nanocrystal					306:316	Traditional cellulose nanocrystal	284:316	Traditional cellulose nanocrystal (CNC) blend nanocomposite films	284:348	Traditional cellulose nanocrystal (CNC) blend nanocomposite films typically lose their coloration and display weak mechanical performance due to poor load transfer between needle-like nanocrystals and the collapse of a twisted organization.
32910639	1	82	theme	biopolymer	170:179	arg1	composites					181:190	chiral nematic biopolymer composites	155:190	chiral nematic biopolymer composites	155:190	Attaining high mechanical strength and flexibility for chiral nematic biopolymer composites without compromising their vivid optical iridescence is an intriguing but challenging task.
34090134	11	0	theme	variation	1390:1398	arg1	trend					1369:1373	The trend	1365:1373	The trend of the acidity variation with different substituents on the phenyl group	1365:1446	The trend of the acidity variation with different substituents on the phenyl group was confirmed by the retention factors of acetone on the CSPs.
34090134	9	1	theme	lower	1168:1172	arg1	factors					1185:1191	lower separation factors	1168:1191	lower separation factors	1168:1191	The CS with 3-Me enantiomerically recognized more analytes than the one with 3-Cl, but showed lower separation factors in more enantioseparations.
34090134	7	2	theme	good	866:869	arg1	capability					889:898	a good enantioseparation capability	864:898	a good enantioseparation capability to the tested chiral analytes	864:928	The CSPs demonstrated a good enantioseparation capability to the tested chiral analytes.
34090134	12	3	theme	CSs	1937:1939	arg1	related					1961:1967	related	1961:1967	related	1961:1967	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	3	theme	CSs	1937:1939	arg1	role					1925:1928	the role	1921:1928	the role of the CSs in HB formation	1921:1955	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	11	4	theme	phenyl	1435:1440	arg1	group					1442:1446	the phenyl group	1431:1446	the phenyl group	1431:1446	The trend of the acidity variation with different substituents on the phenyl group was confirmed by the retention factors of acetone on the CSPs.
34090134	12	5	theme	HB	1944:1945	arg1	formation					1947:1955	HB formation	1944:1955	HB formation	1944:1955	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	6	theme	lone	1755:1758	arg1	electrons					1767:1775	lone paired electrons	1755:1775	lone paired electrons	1755:1775	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	9	7	theme	more	1196:1199	arg1	enantioseparations					1201:1218	more enantioseparations	1196:1218	more enantioseparations	1196:1218	The CS with 3-Me enantiomerically recognized more analytes than the one with 3-Cl, but showed lower separation factors in more enantioseparations.
34090134	2	8	theme	chitosan	276:283	arg1	s					334:334	six chitosan 3,6-bis(phenylcarbamate)-2-(cyclohexylmethylurea)s	272:334	six chitosan 3,6-bis(phenylcarbamate)-2-(cyclohexylmethylurea)s	272:334	Hence, six chitosan 3,6-bis(phenylcarbamate)-2-(cyclohexylmethylurea)s were synthesized and characterized.
34090134	1	9	theme	chitosan-type	182:194	arg1	CSs					214:216	CSs	214:216	CSs	214:216	The goal of the present study was to disclose the interactions between chitosan-type chiral selectors (CSs) and chiral analytes during enantioseparation.
34090134	1	9	theme	chitosan-type	182:194	arg1	selectors					203:211	chitosan-type chiral selectors	182:211	chitosan-type chiral selectors (CSs)	182:217	The goal of the present study was to disclose the interactions between chitosan-type chiral selectors (CSs) and chiral analytes during enantioseparation.
34090134	8	10	with	counterparts	1019:1030	arg1	3,5-diMe					1037:1044	3,5-diMe	1037:1044	3,5-diMe	1037:1044	In enantioselectivity, the CSs with 3,5-diCl and with 4-Me roughly were better than the counterparts with 3,5-diMe and with 4-Cl respectively.
34090134	8	10	with	counterparts	1019:1030	arg1	4-Cl					1055:1058	4-Cl	1055:1058	4-Cl respectively	1055:1071	In enantioselectivity, the CSs with 3,5-diCl and with 4-Me roughly were better than the counterparts with 3,5-diMe and with 4-Cl respectively.
34090134	13	11	theme	chiral	2230:2235	arg1	mechanism					2249:2257	chiral recognition mechanism	2230:2257	chiral recognition mechanism	2230:2257	Based on the discussion, chiral recognition mechanism could be understood in more detail.
34090134	12	12	theme	reactive	1728:1735	arg1	hydrogen					1737:1744	reactive hydrogen	1728:1744	reactive hydrogen	1728:1744	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	11	13	from	substituents	1415:1426	arg1	group					1442:1446	the phenyl group	1431:1446	the phenyl group	1431:1446	The trend of the acidity variation with different substituents on the phenyl group was confirmed by the retention factors of acetone on the CSPs.
34090134	12	14	theme	bond	1614:1617	arg1	formation					1592:1600	the formation	1588:1600	the formation of hydrogen bond (HB) in enantioseparation	1588:1643	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	15	theme	formation	2029:2037	arg1	patterns					2014:2021	the patterns	2010:2021	the patterns of HB formation between the CSs	2010:2053	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	15	theme	formation	2029:2037	arg1	analytes					2059:2066	analytes	2059:2066	analytes	2059:2066	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	16	theme	counterparts	1574:1585	arg1	set					1563:1565	every set	1557:1565	every set of the counterparts	1557:1585	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	16	theme	counterparts	1574:1585	arg1	counterparts					1574:1585	the counterparts	1570:1585	the counterparts	1570:1585	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	17	theme	hydrogen	2000:2007	arg1	acidity					1976:1982	the acidity	1972:1982	the acidity of the reactive hydrogen	1972:2007	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	5	18	theme	sets	663:666	arg1	counterparts					637:648	The counterparts	633:648	The counterparts of the three sets	633:666	The counterparts of the three sets were 3,5-diMe versus 3,5-diCl, 4-Me versus 4-Cl and 3-Me versus 3-Cl.
34090134	5	18	theme	sets	663:666	arg1	3,5-diMe					673:680	3,5-diMe	673:680	3,5-diMe	673:680	The counterparts of the three sets were 3,5-diMe versus 3,5-diCl, 4-Me versus 4-Cl and 3-Me versus 3-Cl.
34090134	1	19	theme	present	127:133	arg1	study					135:139	the present study	123:139	the present study	123:139	The goal of the present study was to disclose the interactions between chitosan-type chiral selectors (CSs) and chiral analytes during enantioseparation.
34090134	12	20	theme	chiral	1704:1709	arg1	analytes					1711:1718	chiral analytes	1704:1718	chiral analytes without reactive hydrogen	1704:1744	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	14	21	dep	strategy	2308:2315	arg1	improve					2320:2326	improve	2320:2326	to improve enantioseparation capability of a CSP by introducing a substituent onto phenyl group	2317:2411	Besides, the strategy to improve enantioseparation capability of a CSP by introducing a substituent onto phenyl group was clarified and further comprehended.
34090134	0	22	theme	stationary	67:76	arg1	phases					78:83	chiral stationary phases	60:83	chiral stationary phases	60:83	The interactions between chiral analytes and chitosan-based chiral stationary phases during enantioseparation.
34090134	12	23	dep	nature	2140:2145	arg1	addition					2124:2131	addition	2124:2131	addition	2124:2131	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	24	theme	substituents	2171:2182	arg1	position					2159:2166	position	2159:2166	position	2159:2166	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	24	theme	substituents	2171:2182	arg1	number					2148:2153	number	2148:2153	number	2148:2153	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	24	theme	substituents	2171:2182	arg1	nature					2140:2145	the nature	2136:2145	the nature	2136:2145	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	25	from	substituents	2171:2182	arg1	group					2198:2202	the phenyl group	2187:2202	the phenyl group	2187:2202	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	10	26	theme	hydrogen	1246:1253	arg1	acidity					1225:1231	The acidity	1221:1231	The acidity of the amide hydrogen in the phenylcarbamates	1221:1277	The acidity of the amide hydrogen in the phenylcarbamates was investigated with density functional theory calculations and 1H NMR measurements.
34090134	12	27	from	position	2159:2166	arg1	group					2198:2202	the phenyl group	2187:2202	the phenyl group	2187:2202	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	10	28	theme	theory	1320:1325	arg1	calculations					1327:1338	density functional theory calculations	1301:1338	density functional theory calculations	1301:1338	The acidity of the amide hydrogen in the phenylcarbamates was investigated with density functional theory calculations and 1H NMR measurements.
34090134	12	29	theme	analytes	1545:1552	arg1	factors					1534:1540	the retention factors	1520:1540	the retention factors of analytes on every set of the counterparts	1520:1585	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	3	30	theme	coated-type	449:459	arg1	phases					479:484	the corresponding coated-type chiral stationary phases	431:484	the corresponding coated-type chiral stationary phases (CSPs)	431:491	These chitosan derivatives were employed as CSs with which the corresponding coated-type chiral stationary phases (CSPs) were prepared.
34090134	3	30	theme	coated-type	449:459	arg1	CSPs					487:490	CSPs	487:490	CSPs	487:490	These chitosan derivatives were employed as CSs with which the corresponding coated-type chiral stationary phases (CSPs) were prepared.
34090134	4	31	from	group	579:583	arg1	position					536:543	position	536:543	position	536:543	According to the nature and position of the substituents on the phenyl group, the CSs and CSPs were divided into three sets.
34090134	4	31	from	group	579:583	arg1	nature					525:530	nature	525:530	nature	525:530	According to the nature and position of the substituents on the phenyl group, the CSs and CSPs were divided into three sets.
34090134	12	32	from	analytes	1545:1552	arg1	set					1563:1565	every set	1557:1565	every set of the counterparts	1557:1585	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	32	from	analytes	1545:1552	arg1	counterparts					1574:1585	the counterparts	1570:1585	the counterparts	1570:1585	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	33	with	analytes	1886:1893	arg1	hydrogen					1911:1918	a reactive hydrogen	1900:1918	a reactive hydrogen	1900:1918	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	34	from	factors	1534:1540	arg1	set					1563:1565	every set	1557:1565	every set of the counterparts	1557:1585	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	34	from	factors	1534:1540	arg1	counterparts					1574:1585	the counterparts	1570:1585	the counterparts	1570:1585	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	3	35	theme	chitosan	378:385	arg1	CSs					416:418	CSs	416:418	CSs with which the corresponding coated-type chiral stationary phases (CSPs) were prepared	416:505	These chitosan derivatives were employed as CSs with which the corresponding coated-type chiral stationary phases (CSPs) were prepared.
34090134	3	35	theme	chitosan	378:385	arg1	derivatives					387:397	These chitosan derivatives	372:397	These chitosan derivatives	372:397	These chitosan derivatives were employed as CSs with which the corresponding coated-type chiral stationary phases (CSPs) were prepared.
34090134	4	36	theme	substituents	552:563	arg1	position					536:543	position	536:543	position	536:543	According to the nature and position of the substituents on the phenyl group, the CSs and CSPs were divided into three sets.
34090134	4	36	theme	substituents	552:563	arg1	nature					525:530	nature	525:530	nature	525:530	According to the nature and position of the substituents on the phenyl group, the CSs and CSPs were divided into three sets.
34090134	12	37	dep	follows	1666:1672	arg1	donors					1816:1821	HB donors	1813:1821	HB donors	1813:1821	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	37	dep	follows	1666:1672	arg1	analytes					1831:1838	the analytes	1827:1838	the analytes	1827:1838	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	37	dep	follows	1666:1672	arg1	acceptors					1848:1856	HB acceptors	1845:1856	HB acceptors	1845:1856	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	37	dep	follows	1666:1672	arg1	N‒Hs					1792:1795	the carbamate N‒Hs	1778:1795	the carbamate N‒Hs in the CSs	1778:1806	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	4	38	from	substituents	552:563	arg1	group					579:583	the phenyl group	568:583	the phenyl group	568:583	According to the nature and position of the substituents on the phenyl group, the CSs and CSPs were divided into three sets.
34090134	10	39	theme	functional	1309:1318	arg1	calculations					1327:1338	density functional theory calculations	1301:1338	density functional theory calculations	1301:1338	The acidity of the amide hydrogen in the phenylcarbamates was investigated with density functional theory calculations and 1H NMR measurements.
34090134	11	40	theme	retention	1469:1477	arg1	factors					1479:1485	the retention factors	1465:1485	the retention factors of acetone	1465:1496	The trend of the acidity variation with different substituents on the phenyl group was confirmed by the retention factors of acetone on the CSPs.
34090134	3	41	theme	stationary	468:477	arg1	phases					479:484	the corresponding coated-type chiral stationary phases	431:484	the corresponding coated-type chiral stationary phases (CSPs)	431:491	These chitosan derivatives were employed as CSs with which the corresponding coated-type chiral stationary phases (CSPs) were prepared.
34090134	3	41	theme	stationary	468:477	arg1	CSPs					487:490	CSPs	487:490	CSPs	487:490	These chitosan derivatives were employed as CSs with which the corresponding coated-type chiral stationary phases (CSPs) were prepared.
34090134	4	42	dep	nature	525:530	arg1	the					521:523	the	521:523	the	521:523	According to the nature and position of the substituents on the phenyl group, the CSs and CSPs were divided into three sets.
34090134	12	43	theme	retention	1524:1532	arg1	factors					1534:1540	the retention factors	1520:1540	the retention factors of analytes on every set of the counterparts	1520:1585	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	8	44	with	CSs	958:960	arg1	4-Me					985:988	4-Me	985:988	4-Me roughly	985:996	In enantioselectivity, the CSs with 3,5-diCl and with 4-Me roughly were better than the counterparts with 3,5-diMe and with 4-Cl respectively.
34090134	8	44	with	CSs	958:960	arg1	3,5-diCl					967:974	3,5-diCl	967:974	3,5-diCl	967:974	In enantioselectivity, the CSs with 3,5-diCl and with 4-Me roughly were better than the counterparts with 3,5-diMe and with 4-Cl respectively.
34090134	12	45	theme	mobile	2106:2111	arg1	phases					2113:2118	mobile phases	2106:2118	mobile phases	2106:2118	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	46	from	group	2198:2202	arg1	position					2159:2166	position	2159:2166	position	2159:2166	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	46	from	group	2198:2202	arg1	number					2148:2153	number	2148:2153	number	2148:2153	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	46	from	group	2198:2202	arg1	nature					2140:2145	the nature	2136:2145	the nature	2136:2145	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	10	47	theme	1H	1344:1345	arg1	measurements					1351:1362	1H NMR measurements	1344:1362	1H NMR measurements	1344:1362	The acidity of the amide hydrogen in the phenylcarbamates was investigated with density functional theory calculations and 1H NMR measurements.
34090134	1	48	theme	chiral	223:228	arg1	analytes					230:237	chiral analytes	223:237	chiral analytes	223:237	The goal of the present study was to disclose the interactions between chitosan-type chiral selectors (CSs) and chiral analytes during enantioseparation.
34090134	6	49	theme	liquid	819:824	arg1	chromatography					826:839	high-performance liquid chromatography	802:839	high-performance liquid chromatography	802:839	The enantioseparation capability of the CSPs was evaluated with high-performance liquid chromatography.
34090134	12	50	from	N‒Hs	1792:1795	arg1	CSs					1804:1806	the CSs	1800:1806	the CSs	1800:1806	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	7	51	theme	tested	907:912	arg1	analytes					921:928	the tested chiral analytes	903:928	the tested chiral analytes	903:928	The CSPs demonstrated a good enantioseparation capability to the tested chiral analytes.
34090134	0	52	theme	chiral	60:65	arg1	phases					78:83	chiral stationary phases	60:83	chiral stationary phases	60:83	The interactions between chiral analytes and chitosan-based chiral stationary phases during enantioseparation.
34090134	12	53	theme	carbamate	1782:1790	arg1	donors					1816:1821	HB donors	1813:1821	HB donors	1813:1821	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	53	theme	carbamate	1782:1790	arg1	N‒Hs					1792:1795	the carbamate N‒Hs	1778:1795	the carbamate N‒Hs in the CSs	1778:1806	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	11	54	theme	acidity	1382:1388	arg1	variation					1390:1398	the acidity variation	1378:1398	the acidity variation	1378:1398	The trend of the acidity variation with different substituents on the phenyl group was confirmed by the retention factors of acetone on the CSPs.
34090134	4	55	from	nature	525:530	arg1	group					579:583	the phenyl group	568:583	the phenyl group	568:583	According to the nature and position of the substituents on the phenyl group, the CSs and CSPs were divided into three sets.
34090134	12	56	from	formation	1592:1600	arg1	enantioseparation					1627:1643	enantioseparation	1627:1643	enantioseparation	1627:1643	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	9	57	with	one	1142:1144	arg1	3-Cl					1151:1154	3-Cl	1151:1154	3-Cl	1151:1154	The CS with 3-Me enantiomerically recognized more analytes than the one with 3-Cl, but showed lower separation factors in more enantioseparations.
34090134	9	58	theme	separation	1174:1183	arg1	factors					1185:1191	lower separation factors	1168:1191	lower separation factors	1168:1191	The CS with 3-Me enantiomerically recognized more analytes than the one with 3-Cl, but showed lower separation factors in more enantioseparations.
34090134	2	59	theme	phenylcarbamate	293:307	arg1	s					334:334	six chitosan 3,6-bis(phenylcarbamate)-2-(cyclohexylmethylurea)s	272:334	six chitosan 3,6-bis(phenylcarbamate)-2-(cyclohexylmethylurea)s	272:334	Hence, six chitosan 3,6-bis(phenylcarbamate)-2-(cyclohexylmethylurea)s were synthesized and characterized.
34090134	7	60	theme	enantioseparation	871:887	arg1	capability					889:898	a good enantioseparation capability	864:898	a good enantioseparation capability to the tested chiral analytes	864:928	The CSPs demonstrated a good enantioseparation capability to the tested chiral analytes.
34090134	14	61	theme	CSP	2362:2364	arg1	capability					2346:2355	enantioseparation capability	2328:2355	enantioseparation capability of a CSP	2328:2364	Besides, the strategy to improve enantioseparation capability of a CSP by introducing a substituent onto phenyl group was clarified and further comprehended.
34090134	12	62	theme	paired	1760:1765	arg1	electrons					1767:1775	lone paired electrons	1755:1775	lone paired electrons	1755:1775	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	10	63	from	acidity	1225:1231	arg1	phenylcarbamates					1262:1277	the phenylcarbamates	1258:1277	the phenylcarbamates	1258:1277	The acidity of the amide hydrogen in the phenylcarbamates was investigated with density functional theory calculations and 1H NMR measurements.
34090134	6	64	theme	CSPs	778:781	arg1	capability					760:769	The enantioseparation capability	738:769	The enantioseparation capability of the CSPs	738:781	The enantioseparation capability of the CSPs was evaluated with high-performance liquid chromatography.
34090134	2	65	theme	3,6-bis	285:291	arg1	s					334:334	six chitosan 3,6-bis(phenylcarbamate)-2-(cyclohexylmethylurea)s	272:334	six chitosan 3,6-bis(phenylcarbamate)-2-(cyclohexylmethylurea)s	272:334	Hence, six chitosan 3,6-bis(phenylcarbamate)-2-(cyclohexylmethylurea)s were synthesized and characterized.
34090134	12	66	from	nature	2140:2145	arg1	group					2198:2202	the phenyl group	2187:2202	the phenyl group	2187:2202	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	2	67	theme	cyclohexylmethylurea	313:332	arg1	s					334:334	six chitosan 3,6-bis(phenylcarbamate)-2-(cyclohexylmethylurea)s	272:334	six chitosan 3,6-bis(phenylcarbamate)-2-(cyclohexylmethylurea)s	272:334	Hence, six chitosan 3,6-bis(phenylcarbamate)-2-(cyclohexylmethylurea)s were synthesized and characterized.
34090134	1	68	theme	chiral	196:201	arg1	CSs					214:216	CSs	214:216	CSs	214:216	The goal of the present study was to disclose the interactions between chitosan-type chiral selectors (CSs) and chiral analytes during enantioseparation.
34090134	1	68	theme	chiral	196:201	arg1	selectors					203:211	chitosan-type chiral selectors	182:211	chitosan-type chiral selectors (CSs)	182:217	The goal of the present study was to disclose the interactions between chitosan-type chiral selectors (CSs) and chiral analytes during enantioseparation.
34090134	12	69	theme	hydrogen	1605:1612	arg1	HB					1620:1621	HB	1620:1621	HB	1620:1621	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	69	theme	hydrogen	1605:1612	arg1	bond					1614:1617	hydrogen bond	1605:1617	hydrogen bond (HB)	1605:1622	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	6	70	theme	enantioseparation	742:758	arg1	capability					760:769	The enantioseparation capability	738:769	The enantioseparation capability of the CSPs	738:781	The enantioseparation capability of the CSPs was evaluated with high-performance liquid chromatography.
34090134	2	71	theme	-2-	309:311	arg1	s					334:334	six chitosan 3,6-bis(phenylcarbamate)-2-(cyclohexylmethylurea)s	272:334	six chitosan 3,6-bis(phenylcarbamate)-2-(cyclohexylmethylurea)s	272:334	Hence, six chitosan 3,6-bis(phenylcarbamate)-2-(cyclohexylmethylurea)s were synthesized and characterized.
34090134	12	72	theme	HB	2026:2027	arg1	formation					2029:2037	HB formation	2026:2037	HB formation between the CSs	2026:2053	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	73	theme	reactive	1902:1909	arg1	hydrogen					1911:1918	a reactive hydrogen	1900:1918	a reactive hydrogen	1900:1918	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	74	theme	reactive	1991:1998	arg1	hydrogen					2000:2007	the reactive hydrogen	1987:2007	the reactive hydrogen	1987:2007	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	10	75	theme	amide	1240:1244	arg1	hydrogen					1246:1253	the amide hydrogen	1236:1253	the amide hydrogen	1236:1253	The acidity of the amide hydrogen in the phenylcarbamates was investigated with density functional theory calculations and 1H NMR measurements.
34090134	1	76	theme	study	135:139	arg1	goal					115:118	The goal	111:118	The goal of the present study	111:139	The goal of the present study was to disclose the interactions between chitosan-type chiral selectors (CSs) and chiral analytes during enantioseparation.
34090134	11	77	theme	acetone	1490:1496	arg1	factors					1479:1485	the retention factors	1465:1485	the retention factors of acetone	1465:1496	The trend of the acidity variation with different substituents on the phenyl group was confirmed by the retention factors of acetone on the CSPs.
34090134	9	78	with	CS	1078:1079	arg1	3-Me					1086:1089	3-Me	1086:1089	3-Me	1086:1089	The CS with 3-Me enantiomerically recognized more analytes than the one with 3-Cl, but showed lower separation factors in more enantioseparations.
34090134	12	79	theme	phenyl	2191:2196	arg1	group					2198:2202	the phenyl group	2187:2202	the phenyl group	2187:2202	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	14	80	theme	phenyl	2400:2405	arg1	group					2407:2411	phenyl group	2400:2411	phenyl group	2400:2411	Besides, the strategy to improve enantioseparation capability of a CSP by introducing a substituent onto phenyl group was clarified and further comprehended.
34090134	12	81	from	set	1563:1565	arg1	factors					1534:1540	the retention factors	1520:1540	the retention factors of analytes on every set of the counterparts	1520:1585	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	11	82	with	trend	1369:1373	arg1	substituents					1415:1426	different substituents	1405:1426	different substituents on the phenyl group	1405:1446	The trend of the acidity variation with different substituents on the phenyl group was confirmed by the retention factors of acetone on the CSPs.
34090134	10	83	theme	density	1301:1307	arg1	calculations					1327:1338	density functional theory calculations	1301:1338	density functional theory calculations	1301:1338	The acidity of the amide hydrogen in the phenylcarbamates was investigated with density functional theory calculations and 1H NMR measurements.
34090134	3	84	theme	chiral	461:466	arg1	phases					479:484	the corresponding coated-type chiral stationary phases	431:484	the corresponding coated-type chiral stationary phases (CSPs)	431:491	These chitosan derivatives were employed as CSs with which the corresponding coated-type chiral stationary phases (CSPs) were prepared.
34090134	3	84	theme	chiral	461:466	arg1	CSPs					487:490	CSPs	487:490	CSPs	487:490	These chitosan derivatives were employed as CSs with which the corresponding coated-type chiral stationary phases (CSPs) were prepared.
34090134	12	85	theme	phases	2113:2118	arg1	compositions					2090:2101	compositions	2090:2101	compositions of mobile phases	2090:2118	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	3	86	theme	corresponding	435:447	arg1	phases					479:484	the corresponding coated-type chiral stationary phases	431:484	the corresponding coated-type chiral stationary phases (CSPs)	431:491	These chitosan derivatives were employed as CSs with which the corresponding coated-type chiral stationary phases (CSPs) were prepared.
34090134	3	86	theme	corresponding	435:447	arg1	CSPs					487:490	CSPs	487:490	CSPs	487:490	These chitosan derivatives were employed as CSs with which the corresponding coated-type chiral stationary phases (CSPs) were prepared.
34090134	0	87	theme	chiral	25:30	arg1	analytes					32:39	chiral analytes	25:39	chiral analytes	25:39	The interactions between chiral analytes and chitosan-based chiral stationary phases during enantioseparation.
34090134	4	88	theme	phenyl	572:577	arg1	group					579:583	the phenyl group	568:583	the phenyl group	568:583	According to the nature and position of the substituents on the phenyl group, the CSs and CSPs were divided into three sets.
34090134	9	89	theme	more	1119:1122	arg1	analytes					1124:1131	more analytes	1119:1131	more analytes	1119:1131	The CS with 3-Me enantiomerically recognized more analytes than the one with 3-Cl, but showed lower separation factors in more enantioseparations.
34090134	12	90	theme	HB	1845:1846	arg1	analytes					1831:1838	the analytes	1827:1838	the analytes	1827:1838	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	90	theme	HB	1845:1846	arg1	acceptors					1848:1856	HB acceptors	1845:1856	HB acceptors	1845:1856	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	91	from	role	1925:1928	arg1	formation					1947:1955	HB formation	1944:1955	HB formation	1944:1955	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	14	92	theme	enantioseparation	2328:2344	arg1	capability					2346:2355	enantioseparation capability	2328:2355	enantioseparation capability of a CSP	2328:2364	Besides, the strategy to improve enantioseparation capability of a CSP by introducing a substituent onto phenyl group was clarified and further comprehended.
34090134	12	93	from	number	2148:2153	arg1	group					2198:2202	the phenyl group	2187:2202	the phenyl group	2187:2202	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	4	94	from	position	536:543	arg1	group					579:583	the phenyl group	568:583	the phenyl group	568:583	According to the nature and position of the substituents on the phenyl group, the CSs and CSPs were divided into three sets.
34090134	12	95	theme	HB	1813:1814	arg1	donors					1816:1821	HB donors	1813:1821	HB donors	1813:1821	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	12	95	theme	HB	1813:1814	arg1	N‒Hs					1792:1795	the carbamate N‒Hs	1778:1795	the carbamate N‒Hs in the CSs	1778:1806	Compared the retention factors of analytes on every set of the counterparts, the formation of hydrogen bond (HB) in enantioseparation could be outlined as follows: when the CSs interacted with chiral analytes without reactive hydrogen but with lone paired electrons, the carbamate N‒Hs in the CSs were HB donors and the analytes were HB acceptors; if the CSs interacted with analytes with a reactive hydrogen, the role of the CSs in HB formation was related to the acidity of the reactive hydrogen; the patterns of HB formation between the CSs and analytes were also impacted by compositions of mobile phases, in addition to the nature, number and position of substituents on the phenyl group.
34090134	13	96	theme	more	2282:2285	arg1	detail					2287:2292	more detail	2282:2292	more detail	2282:2292	Based on the discussion, chiral recognition mechanism could be understood in more detail.
34090134	10	97	theme	NMR	1347:1349	arg1	measurements					1351:1362	1H NMR measurements	1344:1362	1H NMR measurements	1344:1362	The acidity of the amide hydrogen in the phenylcarbamates was investigated with density functional theory calculations and 1H NMR measurements.
34090134	7	98	theme	chiral	914:919	arg1	analytes					921:928	the tested chiral analytes	903:928	the tested chiral analytes	903:928	The CSPs demonstrated a good enantioseparation capability to the tested chiral analytes.
34090134	13	99	theme	recognition	2237:2247	arg1	mechanism					2249:2257	chiral recognition mechanism	2230:2257	chiral recognition mechanism	2230:2257	Based on the discussion, chiral recognition mechanism could be understood in more detail.
34090134	6	100	theme	high-performance	802:817	arg1	chromatography					826:839	high-performance liquid chromatography	802:839	high-performance liquid chromatography	802:839	The enantioseparation capability of the CSPs was evaluated with high-performance liquid chromatography.
34090134	11	101	theme	different	1405:1413	arg1	substituents					1415:1426	different substituents	1405:1426	different substituents on the phenyl group	1405:1446	The trend of the acidity variation with different substituents on the phenyl group was confirmed by the retention factors of acetone on the CSPs.
34857258	2	0	theme	osteogenic	514:523	arg1	construct					525:533	an osteogenic construct	511:533	an osteogenic construct made of hydrogel nanocomposites	511:565	Here, 3D printing is employed for the fabrication of an osteogenic construct made of hydrogel nanocomposites.
34857258	8	1	from	relation	1573:1580	arg1	biocompatibility					1475:1490	the superior biocompatibility	1462:1490	the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation	1462:1622	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	9	2	theme	effective	1701:1709	arg1	capability					1725:1734	effective drug delivery capability	1701:1734	effective drug delivery capability	1701:1734	Taken together, given the achieved structural and biological properties and effective drug delivery capability, the hydrogel nanocomposites show promising potential for bone tissue engineering.
34857258	8	3	theme	bioactivity	1508:1518	arg1	biocompatibility					1475:1490	the superior biocompatibility	1462:1490	the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation	1462:1622	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	5	4	theme	constructs	928:937	arg1	stiffness					911:919	the mechanical stiffness	896:919	the mechanical stiffness of the constructs	896:937	The MSNs not only improve the mechanical stiffness of the constructs but also induce formation of an apatite layer when the construct is immersed in simulated body fluid (SBF), thereby promoting cell adhesion and proliferation.
34857258	0	5	theme	phytotherapeutic	77:92	arg1	icariin					94:100	phytotherapeutic icariin	77:100	phytotherapeutic icariin	77:100	3D printing of alginate dialdehyde-gelatin (ADA-GEL) hydrogels incorporating phytotherapeutic icariin loaded mesoporous SiO2-CaO nanoparticles for bone tissue engineering.
34857258	9	6	dep	show	1765:1768	arg1	Taken					1625:1629	Taken	1625:1629	Taken together	1625:1638	Taken together, given the achieved structural and biological properties and effective drug delivery capability, the hydrogel nanocomposites show promising potential for bone tissue engineering.
34857258	9	7	theme	drug	1711:1714	arg1	capability					1725:1734	effective drug delivery capability	1701:1734	effective drug delivery capability	1701:1734	Taken together, given the achieved structural and biological properties and effective drug delivery capability, the hydrogel nanocomposites show promising potential for bone tissue engineering.
34857258	3	8	theme	mesoporous	689:698	arg1	MSNs					729:732	MSNs	729:732	MSNs	729:732	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	3	8	theme	mesoporous	689:698	arg1	nanoparticles					714:726	i.e. mesoporous silica-calcia nanoparticles	684:726	i.e. mesoporous silica-calcia nanoparticles (MSNs)	684:733	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	3	8	theme	mesoporous	689:698	arg1	nanoparticles					669:681	bioactive glass nanoparticles	653:681	bioactive glass nanoparticles	653:681	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	8	9	theme	effect	1552:1557	arg1	biocompatibility					1475:1490	the superior biocompatibility	1462:1490	the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation	1462:1622	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	3	10	theme	silica-calcia	700:712	arg1	MSNs					729:732	MSNs	729:732	MSNs	729:732	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	3	10	theme	silica-calcia	700:712	arg1	nanoparticles					714:726	i.e. mesoporous silica-calcia nanoparticles	684:726	i.e. mesoporous silica-calcia nanoparticles (MSNs)	684:733	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	3	10	theme	silica-calcia	700:712	arg1	nanoparticles					669:681	bioactive glass nanoparticles	653:681	bioactive glass nanoparticles	653:681	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	0	11	theme	mesoporous	109:118	arg1	nanoparticles					129:141	mesoporous SiO2-CaO nanoparticles	109:141	mesoporous SiO2-CaO nanoparticles for bone tissue engineering	109:169	3D printing of alginate dialdehyde-gelatin (ADA-GEL) hydrogels incorporating phytotherapeutic icariin loaded mesoporous SiO2-CaO nanoparticles for bone tissue engineering.
34857258	5	12	theme	simulated	1019:1027	arg1	SBF					1041:1043	SBF	1041:1043	SBF	1041:1043	The MSNs not only improve the mechanical stiffness of the constructs but also induce formation of an apatite layer when the construct is immersed in simulated body fluid (SBF), thereby promoting cell adhesion and proliferation.
34857258	5	12	theme	simulated	1019:1027	arg1	fluid					1034:1038	simulated body fluid	1019:1038	simulated body fluid (SBF)	1019:1044	The MSNs not only improve the mechanical stiffness of the constructs but also induce formation of an apatite layer when the construct is immersed in simulated body fluid (SBF), thereby promoting cell adhesion and proliferation.
34857258	9	13	theme	delivery	1716:1723	arg1	capability					1725:1734	effective drug delivery capability	1701:1734	effective drug delivery capability	1701:1734	Taken together, given the achieved structural and biological properties and effective drug delivery capability, the hydrogel nanocomposites show promising potential for bone tissue engineering.
34857258	9	14	theme	achieved	1651:1658	arg1	properties					1686:1695	the achieved structural and biological properties	1647:1695	the achieved structural and biological properties	1647:1695	Taken together, given the achieved structural and biological properties and effective drug delivery capability, the hydrogel nanocomposites show promising potential for bone tissue engineering.
34857258	8	15	theme	optimum	1422:1428	arg1	properties					1441:1450	Such optimum biological properties	1417:1450	Such optimum biological properties	1417:1450	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	3	16	theme	icariin	758:764	arg1	loading					767:773	drug (icariin) loading	752:773	drug (icariin) loading	752:773	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	3	17	theme	ADA-GEL	597:603	arg1	hydrogel					606:613	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel	568:613	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel	568:613	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	2	18	theme	hydrogel	543:550	arg1	nanocomposites					552:565	hydrogel nanocomposites	543:565	hydrogel nanocomposites	543:565	Here, 3D printing is employed for the fabrication of an osteogenic construct made of hydrogel nanocomposites.
34857258	8	19	theme	icariin	1562:1568	arg1	bioactivity					1508:1518	the bioactivity	1504:1518	the bioactivity of the MSNs	1504:1530	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	8	19	theme	icariin	1562:1568	arg1	effect					1552:1557	the supportive effect	1537:1557	the supportive effect of icariin in relation to cell proliferation and differentiation	1537:1622	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	8	19	theme	icariin	1562:1568	arg1	ADA-GEL					1495:1501	ADA-GEL	1495:1501	ADA-GEL	1495:1501	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	3	20	theme	Alginate	568:575	arg1	hydrogel					606:613	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel	568:613	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel	568:613	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	7	21	theme	enhanced	1351:1358	arg1	proliferation					1371:1383	enhanced osteoblast proliferation	1351:1383	enhanced osteoblast proliferation	1351:1383	Biocompatibility tests showed that the hydrogel nanocomposites assure enhanced osteoblast proliferation, adhesion, and differentiation.
34857258	0	22	theme	SiO2-CaO	120:127	arg1	nanoparticles					129:141	mesoporous SiO2-CaO nanoparticles	109:141	mesoporous SiO2-CaO nanoparticles for bone tissue engineering	109:169	3D printing of alginate dialdehyde-gelatin (ADA-GEL) hydrogels incorporating phytotherapeutic icariin loaded mesoporous SiO2-CaO nanoparticles for bone tissue engineering.
34857258	3	23	theme	drug	752:755	arg1	loading					767:773	drug (icariin) loading	752:773	drug (icariin) loading	752:773	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	9	24	theme	biological	1675:1684	arg1	properties					1686:1695	the achieved structural and biological properties	1647:1695	the achieved structural and biological properties	1647:1695	Taken together, given the achieved structural and biological properties and effective drug delivery capability, the hydrogel nanocomposites show promising potential for bone tissue engineering.
34857258	3	25	theme	dialdehyde-gelatin	577:594	arg1	hydrogel					606:613	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel	568:613	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel	568:613	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	1	26	theme	better	194:199	arg1	control					201:207	a better control	192:207	a better control over the microstructure of bone restoring constructs	192:260	3D printing enables a better control over the microstructure of bone restoring constructs, addresses the challenges seen in the preparation of patient-specific bone scaffolds, and overcomes the bottlenecks that can appear in delivering drugs/growth factors promoting bone regeneration.
34857258	8	27	theme	superior	1466:1473	arg1	biocompatibility					1475:1490	the superior biocompatibility	1462:1490	the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation	1462:1622	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	9	28	theme	hydrogel	1741:1748	arg1	nanocomposites					1750:1763	the hydrogel nanocomposites	1737:1763	the hydrogel nanocomposites	1737:1763	Taken together, given the achieved structural and biological properties and effective drug delivery capability, the hydrogel nanocomposites show promising potential for bone tissue engineering.
34857258	8	29	theme	Such	1417:1420	arg1	properties					1441:1450	Such optimum biological properties	1417:1450	Such optimum biological properties	1417:1450	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	5	30	theme	mechanical	900:909	arg1	stiffness					911:919	the mechanical stiffness	896:919	the mechanical stiffness of the constructs	896:937	The MSNs not only improve the mechanical stiffness of the constructs but also induce formation of an apatite layer when the construct is immersed in simulated body fluid (SBF), thereby promoting cell adhesion and proliferation.
34857258	0	31	theme	3D	0:1	arg1	printing					3:10	3D printing	0:10	3D printing of alginate dialdehyde-gelatin (ADA-GEL) hydrogels incorporating phytotherapeutic icariin	0:100	3D printing of alginate dialdehyde-gelatin (ADA-GEL) hydrogels incorporating phytotherapeutic icariin loaded mesoporous SiO2-CaO nanoparticles for bone tissue engineering.
34857258	8	32	dep	cell	1585:1588	arg1	differentiation					1608:1622	differentiation	1608:1622	differentiation	1608:1622	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	8	32	dep	cell	1585:1588	arg1	proliferation					1590:1602	proliferation	1590:1602	proliferation	1590:1602	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	5	33	theme	apatite	971:977	arg1	layer					979:983	an apatite layer	968:983	an apatite layer when the construct is immersed in simulated body fluid (SBF), thereby promoting cell adhesion and proliferation	968:1095	The MSNs not only improve the mechanical stiffness of the constructs but also induce formation of an apatite layer when the construct is immersed in simulated body fluid (SBF), thereby promoting cell adhesion and proliferation.
34857258	8	34	from	ADA-GEL	1495:1501	arg1	relation					1573:1580	relation	1573:1580	relation to cell proliferation and differentiation	1573:1622	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	7	35	theme	osteoblast	1360:1369	arg1	proliferation					1371:1383	enhanced osteoblast proliferation	1351:1383	enhanced osteoblast proliferation	1351:1383	Biocompatibility tests showed that the hydrogel nanocomposites assure enhanced osteoblast proliferation, adhesion, and differentiation.
34857258	0	36	theme	alginate	15:22	arg1	hydrogels					53:61	alginate dialdehyde-gelatin (ADA-GEL) hydrogels	15:61	alginate dialdehyde-gelatin (ADA-GEL) hydrogels incorporating phytotherapeutic icariin	15:100	3D printing of alginate dialdehyde-gelatin (ADA-GEL) hydrogels incorporating phytotherapeutic icariin loaded mesoporous SiO2-CaO nanoparticles for bone tissue engineering.
34857258	5	37	theme	body	1029:1032	arg1	SBF					1041:1043	SBF	1041:1043	SBF	1041:1043	The MSNs not only improve the mechanical stiffness of the constructs but also induce formation of an apatite layer when the construct is immersed in simulated body fluid (SBF), thereby promoting cell adhesion and proliferation.
34857258	5	37	theme	body	1029:1032	arg1	fluid					1034:1038	simulated body fluid	1019:1038	simulated body fluid (SBF)	1019:1044	The MSNs not only improve the mechanical stiffness of the constructs but also induce formation of an apatite layer when the construct is immersed in simulated body fluid (SBF), thereby promoting cell adhesion and proliferation.
34857258	0	38	theme	bone	147:150	arg1	engineering					159:169	bone tissue engineering	147:169	bone tissue engineering	147:169	3D printing of alginate dialdehyde-gelatin (ADA-GEL) hydrogels incorporating phytotherapeutic icariin loaded mesoporous SiO2-CaO nanoparticles for bone tissue engineering.
34857258	4	39	theme	composites	780:789	arg1	constructs					838:847	superhydrated composite constructs	814:847	superhydrated composite constructs in a grid structure	814:867	The composites hydrogel is printed as superhydrated composite constructs in a grid structure.
34857258	4	39	theme	composites	780:789	arg1	hydrogel					791:798	The composites hydrogel	776:798	The composites hydrogel	776:798	The composites hydrogel is printed as superhydrated composite constructs in a grid structure.
34857258	2	40	theme	3D	464:465	arg1	printing					467:474	3D printing	464:474	3D printing	464:474	Here, 3D printing is employed for the fabrication of an osteogenic construct made of hydrogel nanocomposites.
34857258	3	41	theme	loading	767:773	arg1	types					743:747	two types	739:747	two types of drug (icariin) loading	739:773	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	9	42	theme	promising	1770:1778	arg1	potential					1780:1788	promising potential	1770:1788	promising potential for bone tissue engineering	1770:1816	Taken together, given the achieved structural and biological properties and effective drug delivery capability, the hydrogel nanocomposites show promising potential for bone tissue engineering.
34857258	1	43	theme	drugs/growth	408:419	arg1	factors					421:427	drugs/growth factors	408:427	drugs/growth factors promoting bone regeneration	408:455	3D printing enables a better control over the microstructure of bone restoring constructs, addresses the challenges seen in the preparation of patient-specific bone scaffolds, and overcomes the bottlenecks that can appear in delivering drugs/growth factors promoting bone regeneration.
34857258	6	44	theme	nanocomposite	1102:1114	arg1	constructs					1116:1125	The nanocomposite constructs	1098:1125	The nanocomposite constructs	1098:1125	The nanocomposite constructs can hold and deliver icariin efficiently, regardless of its incorporation mode, either as loaded into the MSNs or freely distributed within the hydrogel.
34857258	3	45	from	incorporation	636:648	arg1	types					743:747	two types	739:747	two types of drug (icariin) loading	739:773	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	8	46	theme	ADA-GEL	1495:1501	arg1	biocompatibility					1475:1490	the superior biocompatibility	1462:1490	the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation	1462:1622	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	1	47	theme	patient-specific	315:330	arg1	scaffolds					337:345	patient-specific bone scaffolds	315:345	patient-specific bone scaffolds	315:345	3D printing enables a better control over the microstructure of bone restoring constructs, addresses the challenges seen in the preparation of patient-specific bone scaffolds, and overcomes the bottlenecks that can appear in delivering drugs/growth factors promoting bone regeneration.
34857258	4	48	theme	grid	854:857	arg1	structure					859:867	a grid structure	852:867	a grid structure	852:867	The composites hydrogel is printed as superhydrated composite constructs in a grid structure.
34857258	8	49	from	biocompatibility	1475:1490	arg1	relation					1573:1580	relation	1573:1580	relation to cell proliferation and differentiation	1573:1622	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	0	50	theme	dialdehyde-gelatin	24:41	arg1	hydrogels					53:61	alginate dialdehyde-gelatin (ADA-GEL) hydrogels	15:61	alginate dialdehyde-gelatin (ADA-GEL) hydrogels incorporating phytotherapeutic icariin	15:100	3D printing of alginate dialdehyde-gelatin (ADA-GEL) hydrogels incorporating phytotherapeutic icariin loaded mesoporous SiO2-CaO nanoparticles for bone tissue engineering.
34857258	1	51	theme	bone	332:335	arg1	scaffolds					337:345	patient-specific bone scaffolds	315:345	patient-specific bone scaffolds	315:345	3D printing enables a better control over the microstructure of bone restoring constructs, addresses the challenges seen in the preparation of patient-specific bone scaffolds, and overcomes the bottlenecks that can appear in delivering drugs/growth factors promoting bone regeneration.
34857258	0	52	theme	tissue	152:157	arg1	engineering					159:169	bone tissue engineering	147:169	bone tissue engineering	147:169	3D printing of alginate dialdehyde-gelatin (ADA-GEL) hydrogels incorporating phytotherapeutic icariin loaded mesoporous SiO2-CaO nanoparticles for bone tissue engineering.
34857258	3	53	theme	i.e.	684:687	arg1	MSNs					729:732	MSNs	729:732	MSNs	729:732	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	3	53	theme	i.e.	684:687	arg1	nanoparticles					714:726	i.e. mesoporous silica-calcia nanoparticles	684:726	i.e. mesoporous silica-calcia nanoparticles (MSNs)	684:733	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	3	53	theme	i.e.	684:687	arg1	nanoparticles					669:681	bioactive glass nanoparticles	653:681	bioactive glass nanoparticles	653:681	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	8	54	from	bioactivity	1508:1518	arg1	relation					1573:1580	relation	1573:1580	relation to cell proliferation and differentiation	1573:1622	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	1	55	theme	bone	236:239	arg1	constructs					251:260	bone restoring constructs	236:260	bone restoring constructs	236:260	3D printing enables a better control over the microstructure of bone restoring constructs, addresses the challenges seen in the preparation of patient-specific bone scaffolds, and overcomes the bottlenecks that can appear in delivering drugs/growth factors promoting bone regeneration.
34857258	1	56	theme	scaffolds	337:345	arg1	preparation					300:310	the preparation	296:310	the preparation of patient-specific bone scaffolds	296:345	3D printing enables a better control over the microstructure of bone restoring constructs, addresses the challenges seen in the preparation of patient-specific bone scaffolds, and overcomes the bottlenecks that can appear in delivering drugs/growth factors promoting bone regeneration.
34857258	9	57	theme	bone	1794:1797	arg1	engineering					1806:1816	bone tissue engineering	1794:1816	bone tissue engineering	1794:1816	Taken together, given the achieved structural and biological properties and effective drug delivery capability, the hydrogel nanocomposites show promising potential for bone tissue engineering.
34857258	1	58	theme	bone	439:442	arg1	regeneration					444:455	bone regeneration	439:455	bone regeneration	439:455	3D printing enables a better control over the microstructure of bone restoring constructs, addresses the challenges seen in the preparation of patient-specific bone scaffolds, and overcomes the bottlenecks that can appear in delivering drugs/growth factors promoting bone regeneration.
34857258	0	59	theme	hydrogels	53:61	arg1	printing					3:10	3D printing	0:10	3D printing of alginate dialdehyde-gelatin (ADA-GEL) hydrogels incorporating phytotherapeutic icariin	0:100	3D printing of alginate dialdehyde-gelatin (ADA-GEL) hydrogels incorporating phytotherapeutic icariin loaded mesoporous SiO2-CaO nanoparticles for bone tissue engineering.
34857258	8	60	from	effect	1552:1557	arg1	relation					1573:1580	relation	1573:1580	relation to cell proliferation and differentiation	1573:1622	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	0	61	theme	ADA-GEL	44:50	arg1	hydrogels					53:61	alginate dialdehyde-gelatin (ADA-GEL) hydrogels	15:61	alginate dialdehyde-gelatin (ADA-GEL) hydrogels incorporating phytotherapeutic icariin	15:100	3D printing of alginate dialdehyde-gelatin (ADA-GEL) hydrogels incorporating phytotherapeutic icariin loaded mesoporous SiO2-CaO nanoparticles for bone tissue engineering.
34857258	9	62	theme	tissue	1799:1804	arg1	engineering					1806:1816	bone tissue engineering	1794:1816	bone tissue engineering	1794:1816	Taken together, given the achieved structural and biological properties and effective drug delivery capability, the hydrogel nanocomposites show promising potential for bone tissue engineering.
34857258	8	63	theme	MSNs	1527:1530	arg1	bioactivity					1508:1518	the bioactivity	1504:1518	the bioactivity of the MSNs	1504:1530	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	8	63	theme	MSNs	1527:1530	arg1	effect					1552:1557	the supportive effect	1537:1557	the supportive effect of icariin in relation to cell proliferation and differentiation	1537:1622	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	8	63	theme	MSNs	1527:1530	arg1	ADA-GEL					1495:1501	ADA-GEL	1495:1501	ADA-GEL	1495:1501	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	9	64	theme	structural	1660:1669	arg1	properties					1686:1695	the achieved structural and biological properties	1647:1695	the achieved structural and biological properties	1647:1695	Taken together, given the achieved structural and biological properties and effective drug delivery capability, the hydrogel nanocomposites show promising potential for bone tissue engineering.
34857258	6	65	dep	deliver	1140:1146	arg1	loaded					1217:1222	loaded	1217:1222	as loaded into the MSNs	1214:1236	The nanocomposite constructs can hold and deliver icariin efficiently, regardless of its incorporation mode, either as loaded into the MSNs or freely distributed within the hydrogel.
34857258	6	65	dep	deliver	1140:1146	arg1	distributed					1248:1258	distributed	1248:1258	freely distributed within the hydrogel	1241:1278	The nanocomposite constructs can hold and deliver icariin efficiently, regardless of its incorporation mode, either as loaded into the MSNs or freely distributed within the hydrogel.
34857258	4	66	theme	composite	828:836	arg1	constructs					838:847	superhydrated composite constructs	814:847	superhydrated composite constructs in a grid structure	814:867	The composites hydrogel is printed as superhydrated composite constructs in a grid structure.
34857258	4	66	theme	composite	828:836	arg1	hydrogel					791:798	The composites hydrogel	776:798	The composites hydrogel	776:798	The composites hydrogel is printed as superhydrated composite constructs in a grid structure.
34857258	3	67	theme	nanoparticles	669:681	arg1	incorporation					636:648	the incorporation	632:648	the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading	632:773	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	7	68	theme	Biocompatibility	1281:1296	arg1	tests					1298:1302	Biocompatibility tests	1281:1302	Biocompatibility tests	1281:1302	Biocompatibility tests showed that the hydrogel nanocomposites assure enhanced osteoblast proliferation, adhesion, and differentiation.
34857258	5	69	theme	layer	979:983	arg1	formation					955:963	formation	955:963	formation of an apatite layer when the construct is immersed in simulated body fluid (SBF), thereby promoting cell adhesion and proliferation	955:1095	The MSNs not only improve the mechanical stiffness of the constructs but also induce formation of an apatite layer when the construct is immersed in simulated body fluid (SBF), thereby promoting cell adhesion and proliferation.
34857258	1	70	theme	restoring	241:249	arg1	constructs					251:260	bone restoring constructs	236:260	bone restoring constructs	236:260	3D printing enables a better control over the microstructure of bone restoring constructs, addresses the challenges seen in the preparation of patient-specific bone scaffolds, and overcomes the bottlenecks that can appear in delivering drugs/growth factors promoting bone regeneration.
34857258	6	71	theme	incorporation	1187:1199	arg1	mode					1201:1204	its incorporation mode	1183:1204	its incorporation mode	1183:1204	The nanocomposite constructs can hold and deliver icariin efficiently, regardless of its incorporation mode, either as loaded into the MSNs or freely distributed within the hydrogel.
34857258	5	72	theme	cell	1065:1068	arg1	adhesion					1070:1077	cell adhesion	1065:1077	cell adhesion	1065:1077	The MSNs not only improve the mechanical stiffness of the constructs but also induce formation of an apatite layer when the construct is immersed in simulated body fluid (SBF), thereby promoting cell adhesion and proliferation.
34857258	4	73	theme	superhydrated	814:826	arg1	constructs					838:847	superhydrated composite constructs	814:847	superhydrated composite constructs in a grid structure	814:867	The composites hydrogel is printed as superhydrated composite constructs in a grid structure.
34857258	4	73	theme	superhydrated	814:826	arg1	hydrogel					791:798	The composites hydrogel	776:798	The composites hydrogel	776:798	The composites hydrogel is printed as superhydrated composite constructs in a grid structure.
34857258	1	74	theme	constructs	251:260	arg1	microstructure					218:231	the microstructure	214:231	the microstructure of bone restoring constructs	214:260	3D printing enables a better control over the microstructure of bone restoring constructs, addresses the challenges seen in the preparation of patient-specific bone scaffolds, and overcomes the bottlenecks that can appear in delivering drugs/growth factors promoting bone regeneration.
34857258	7	75	theme	hydrogel	1320:1327	arg1	nanocomposites					1329:1342	the hydrogel nanocomposites	1316:1342	the hydrogel nanocomposites	1316:1342	Biocompatibility tests showed that the hydrogel nanocomposites assure enhanced osteoblast proliferation, adhesion, and differentiation.
34857258	3	76	theme	bioactive	653:661	arg1	nanoparticles					714:726	i.e. mesoporous silica-calcia nanoparticles	684:726	i.e. mesoporous silica-calcia nanoparticles (MSNs)	684:733	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	3	76	theme	bioactive	653:661	arg1	nanoparticles					669:681	bioactive glass nanoparticles	653:681	bioactive glass nanoparticles	653:681	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	4	77	from	constructs	838:847	arg1	structure					859:867	a grid structure	852:867	a grid structure	852:867	The composites hydrogel is printed as superhydrated composite constructs in a grid structure.
34857258	8	78	theme	biological	1430:1439	arg1	properties					1441:1450	Such optimum biological properties	1417:1450	Such optimum biological properties	1417:1450	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	2	79	theme	construct	525:533	arg1	fabrication					496:506	the fabrication	492:506	the fabrication of an osteogenic construct made of hydrogel nanocomposites	492:565	Here, 3D printing is employed for the fabrication of an osteogenic construct made of hydrogel nanocomposites.
34857258	8	80	theme	supportive	1541:1550	arg1	effect					1552:1557	the supportive effect	1537:1557	the supportive effect of icariin in relation to cell proliferation and differentiation	1537:1622	Such optimum biological properties stem from the superior biocompatibility of ADA-GEL, the bioactivity of the MSNs, and the supportive effect of icariin in relation to cell proliferation and differentiation.
34857258	3	81	theme	glass	663:667	arg1	nanoparticles					714:726	i.e. mesoporous silica-calcia nanoparticles	684:726	i.e. mesoporous silica-calcia nanoparticles (MSNs)	684:733	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	3	81	theme	glass	663:667	arg1	nanoparticles					669:681	bioactive glass nanoparticles	653:681	bioactive glass nanoparticles	653:681	Alginate dialdehyde-gelatin (ADA-GEL) hydrogel is reinforced by the incorporation of bioactive glass nanoparticles, i.e. mesoporous silica-calcia nanoparticles (MSNs), in two types of drug (icariin) loading.
34857258	1	82	theme	3D	172:173	arg1	printing					175:182	3D printing	172:182	3D printing	172:182	3D printing enables a better control over the microstructure of bone restoring constructs, addresses the challenges seen in the preparation of patient-specific bone scaffolds, and overcomes the bottlenecks that can appear in delivering drugs/growth factors promoting bone regeneration.
33876546	8	0	theme	different	1287:1295	arg1	hydrolases					1307:1316	different glycoside hydrolases	1287:1316	different glycoside hydrolases	1287:1316	One specific Glaciecola ASV dominated the free-living fraction, possibly persisting on particle-derived oligomers through different glycoside hydrolases.
33876546	5	1	theme	microbial	743:751	arg1	dynamics					753:760	microbial dynamics	743:760	microbial dynamics on alginate and pectin particles	743:793	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	9	2	theme	ammonium	1328:1335	arg1	uptake					1337:1342	Elevated ammonium uptake	1319:1342	Elevated ammonium uptake	1319:1342	Elevated ammonium uptake and metabolism signified nitrogen as an important factor for degrading carbon-rich particles, whereas elevated methylcitrate and glyoxylate cycles suggested nutrient limitation in surrounding waters.
33876546	6	3	theme	alginate	993:1000	arg1	lyases					1002:1007	diverse alginate lyases	985:1007	diverse alginate lyases	985:1007	Corresponding metagenome-assembled genomes (MAGs) expressed diverse alginate lyases, several colocalized in polysaccharide utilization loci.
33876546	11	4	theme	bacterial	1757:1765	arg1	specialization					1773:1786	bacterial niche specialization	1757:1786	bacterial niche specialization	1757:1786	These insights expand our understanding of bacterial niche specialization and the biological carbon pump in macroalgae-rich habitats.
33876546	2	5	theme	different	302:310	arg1	composition					327:337	different polysaccharide composition	302:337	different polysaccharide composition	302:337	However, substrate-specific bacterial dynamics in mixtures of particle types with different polysaccharide composition, as likely occurring in natural habitats, are undescribed.
33876546	11	6	theme	carbon	1807:1812	arg1	pump					1814:1817	the biological carbon pump	1792:1817	the biological carbon pump in macroalgae-rich habitats	1792:1845	These insights expand our understanding of bacterial niche specialization and the biological carbon pump in macroalgae-rich habitats.
33876546	2	7	theme	types	291:295	arg1	mixtures					270:277	mixtures	270:277	mixtures of particle types with different polysaccharide composition	270:337	However, substrate-specific bacterial dynamics in mixtures of particle types with different polysaccharide composition, as likely occurring in natural habitats, are undescribed.
33876546	11	8	theme	specialization	1773:1786	arg1	understanding					1740:1752	our understanding	1736:1752	our understanding of bacterial niche specialization	1736:1786	These insights expand our understanding of bacterial niche specialization and the biological carbon pump in macroalgae-rich habitats.
33876546	11	8	theme	specialization	1773:1786	arg1	pump					1814:1817	the biological carbon pump	1792:1817	the biological carbon pump in macroalgae-rich habitats	1792:1845	These insights expand our understanding of bacterial niche specialization and the biological carbon pump in macroalgae-rich habitats.
33876546	5	9	theme	pectin	778:783	arg1	particles					785:793	pectin particles	778:793	pectin particles	778:793	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	9	10	theme	important	1384:1392	arg1	nitrogen					1369:1376	nitrogen	1369:1376	nitrogen	1369:1376	Elevated ammonium uptake and metabolism signified nitrogen as an important factor for degrading carbon-rich particles, whereas elevated methylcitrate and glyoxylate cycles suggested nutrient limitation in surrounding waters.
33876546	9	10	theme	important	1384:1392	arg1	factor					1394:1399	an important factor	1381:1399	an important factor for degrading carbon-rich particles	1381:1435	Elevated ammonium uptake and metabolism signified nitrogen as an important factor for degrading carbon-rich particles, whereas elevated methylcitrate and glyoxylate cycles suggested nutrient limitation in surrounding waters.
33876546	4	11	theme	free-living	703:713	arg1	counterparts					715:726	their free-living counterparts	697:726	their free-living counterparts	697:726	Amplicon, metagenome and metatranscriptome sequencing revealed that communities on alginate and pectin particles significantly differed from their free-living counterparts.
33876546	2	12	theme	bacterial	248:256	arg1	dynamics					258:265	substrate-specific bacterial dynamics	229:265	substrate-specific bacterial dynamics in mixtures of particle types with different polysaccharide composition	229:337	However, substrate-specific bacterial dynamics in mixtures of particle types with different polysaccharide composition, as likely occurring in natural habitats, are undescribed.
33876546	5	13	from	Psychromonas	911:922	arg1	predominance					814:825	predominance	814:825	predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas	814:922	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	5	13	from	Psychromonas	911:922	arg1	ASVs					858:861	ASVs	858:861	ASVs	858:861	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	5	13	from	Psychromonas	911:922	arg1	variants					848:855	amplicon sequence variants	830:855	amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas	830:922	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	6	14	theme	polysaccharide	1033:1046	arg1	loci					1060:1063	polysaccharide utilization loci	1033:1063	polysaccharide utilization loci	1033:1063	Corresponding metagenome-assembled genomes (MAGs) expressed diverse alginate lyases, several colocalized in polysaccharide utilization loci.
33876546	5	15	with	similar	800:806	arg1	predominance					814:825	predominance	814:825	predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas	814:922	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	9	16	theme	carbon-rich	1415:1425	arg1	particles					1427:1435	carbon-rich particles	1415:1435	carbon-rich particles	1415:1435	Elevated ammonium uptake and metabolism signified nitrogen as an important factor for degrading carbon-rich particles, whereas elevated methylcitrate and glyoxylate cycles suggested nutrient limitation in surrounding waters.
33876546	5	17	from	Psychrobium	895:905	arg1	predominance					814:825	predominance	814:825	predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas	814:922	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	5	17	from	Psychrobium	895:905	arg1	ASVs					858:861	ASVs	858:861	ASVs	858:861	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	5	17	from	Psychrobium	895:905	arg1	variants					848:855	amplicon sequence variants	830:855	amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas	830:922	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	7	18	theme	pectin-degrading	1139:1154	arg1	enzymes					1156:1162	pectin-degrading enzymes	1139:1162	pectin-degrading enzymes	1139:1162	Only a single, low-abundant MAG showed elevated transcript abundances of pectin-degrading enzymes.
33876546	11	19	from	pump	1814:1817	arg1	habitats					1838:1845	macroalgae-rich habitats	1822:1845	macroalgae-rich habitats	1822:1845	These insights expand our understanding of bacterial niche specialization and the biological carbon pump in macroalgae-rich habitats.
33876546	2	20	with	types	291:295	arg1	composition					327:337	different polysaccharide composition	302:337	different polysaccharide composition	302:337	However, substrate-specific bacterial dynamics in mixtures of particle types with different polysaccharide composition, as likely occurring in natural habitats, are undescribed.
33876546	7	21	theme	elevated	1105:1112	arg1	abundances					1125:1134	elevated transcript abundances	1105:1134	elevated transcript abundances of pectin-degrading enzymes	1105:1162	Only a single, low-abundant MAG showed elevated transcript abundances of pectin-degrading enzymes.
33876546	5	22	theme	amplicon	830:837	arg1	ASVs					858:861	ASVs	858:861	ASVs	858:861	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	5	22	theme	amplicon	830:837	arg1	variants					848:855	amplicon sequence variants	830:855	amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas	830:922	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	9	23	theme	elevated	1446:1453	arg1	cycles					1484:1489	elevated methylcitrate and glyoxylate cycles	1446:1489	elevated methylcitrate and glyoxylate cycles	1446:1489	Elevated ammonium uptake and metabolism signified nitrogen as an important factor for degrading carbon-rich particles, whereas elevated methylcitrate and glyoxylate cycles suggested nutrient limitation in surrounding waters.
33876546	7	24	theme	single	1073:1078	arg1	MAG					1094:1096	Only a single, low-abundant MAG	1066:1096	Only a single, low-abundant MAG	1066:1096	Only a single, low-abundant MAG showed elevated transcript abundances of pectin-degrading enzymes.
33876546	10	25	theme	substrate-driven	1653:1668	arg1	dynamics					1670:1677	substrate-driven dynamics	1653:1677	substrate-driven dynamics	1653:1677	The bacterial preference for alginate, whereas pectin primarily served as colonization scaffold, illuminates substrate-driven dynamics within mixed polysaccharide pools.
33876546	0	26	theme	communities	68:78	arg1	succession					15:24	succession	15:24	succession	15:24	Sweet spheres: succession and CAZyme expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	0	26	theme	communities	68:78	arg1	expression					37:46	CAZyme expression	30:46	CAZyme expression	30:46	Sweet spheres: succession and CAZyme expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	0	26	theme	communities	68:78	arg1	spheres					6:12	Sweet spheres	0:12	Sweet spheres: succession and CAZyme expression of marine bacterial communities colonizing a mix of alginate and pectin particles.	0:129	Sweet spheres: succession and CAZyme expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	1	27	theme	important	160:168	arg1	particles					146:154	Polysaccharide particles	131:154	Polysaccharide particles	131:154	Polysaccharide particles are important substrates and microhabitats for marine bacteria.
33876546	1	27	theme	important	160:168	arg1	substrates					170:179	substrates	170:179	substrates	170:179	Polysaccharide particles are important substrates and microhabitats for marine bacteria.
33876546	9	28	dep	signified	1359:1367	arg1	whereas					1438:1444	whereas	1438:1444	whereas	1438:1444	Elevated ammonium uptake and metabolism signified nitrogen as an important factor for degrading carbon-rich particles, whereas elevated methylcitrate and glyoxylate cycles suggested nutrient limitation in surrounding waters.
33876546	5	29	from	Colwellia	884:892	arg1	predominance					814:825	predominance	814:825	predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas	814:922	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	5	29	from	Colwellia	884:892	arg1	ASVs					858:861	ASVs	858:861	ASVs	858:861	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	5	29	from	Colwellia	884:892	arg1	variants					848:855	amplicon sequence variants	830:855	amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas	830:922	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	10	30	theme	polysaccharide	1692:1705	arg1	pools					1707:1711	mixed polysaccharide pools	1686:1711	mixed polysaccharide pools	1686:1711	The bacterial preference for alginate, whereas pectin primarily served as colonization scaffold, illuminates substrate-driven dynamics within mixed polysaccharide pools.
33876546	3	31	theme	gene	457:460	arg1	expression					462:471	gene expression	457:471	gene expression	457:471	Here, we studied the composition, functional diversity and gene expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	9	32	theme	nutrient	1501:1508	arg1	limitation					1510:1519	nutrient limitation	1501:1519	nutrient limitation	1501:1519	Elevated ammonium uptake and metabolism signified nitrogen as an important factor for degrading carbon-rich particles, whereas elevated methylcitrate and glyoxylate cycles suggested nutrient limitation in surrounding waters.
33876546	2	33	from	dynamics	258:265	arg1	mixtures					270:277	mixtures	270:277	mixtures of particle types with different polysaccharide composition	270:337	However, substrate-specific bacterial dynamics in mixtures of particle types with different polysaccharide composition, as likely occurring in natural habitats, are undescribed.
33876546	0	34	theme	Sweet	0:4	arg1	succession					15:24	succession	15:24	succession	15:24	Sweet spheres: succession and CAZyme expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	0	34	theme	Sweet	0:4	arg1	expression					37:46	CAZyme expression	30:46	CAZyme expression	30:46	Sweet spheres: succession and CAZyme expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	0	34	theme	Sweet	0:4	arg1	spheres					6:12	Sweet spheres	0:12	Sweet spheres: succession and CAZyme expression of marine bacterial communities colonizing a mix of alginate and pectin particles.	0:129	Sweet spheres: succession and CAZyme expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	8	35	theme	Glaciecola	1178:1187	arg1	ASV					1189:1191	One specific Glaciecola ASV	1165:1191	One specific Glaciecola ASV	1165:1191	One specific Glaciecola ASV dominated the free-living fraction, possibly persisting on particle-derived oligomers through different glycoside hydrolases.
33876546	3	36	theme	bacterial	483:491	arg1	communities					493:503	marine bacterial communities	476:503	marine bacterial communities colonizing a mix of alginate and pectin particles	476:553	Here, we studied the composition, functional diversity and gene expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	8	37	theme	free-living	1207:1217	arg1	fraction					1219:1226	the free-living fraction	1203:1226	the free-living fraction	1203:1226	One specific Glaciecola ASV dominated the free-living fraction, possibly persisting on particle-derived oligomers through different glycoside hydrolases.
33876546	6	38	theme	metagenome-assembled	939:958	arg1	MAGs					969:972	MAGs	969:972	MAGs	969:972	Corresponding metagenome-assembled genomes (MAGs) expressed diverse alginate lyases, several colocalized in polysaccharide utilization loci.
33876546	6	38	theme	metagenome-assembled	939:958	arg1	genomes					960:966	Corresponding metagenome-assembled genomes	925:966	Corresponding metagenome-assembled genomes (MAGs)	925:973	Corresponding metagenome-assembled genomes (MAGs) expressed diverse alginate lyases, several colocalized in polysaccharide utilization loci.
33876546	0	39	theme	marine	51:56	arg1	communities					68:78	marine bacterial communities	51:78	marine bacterial communities colonizing a mix of alginate and pectin particles	51:128	Sweet spheres: succession and CAZyme expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	10	40	dep	illuminates	1641:1651	arg1	served					1608:1613	served	1608:1613	served as colonization scaffold	1608:1638	The bacterial preference for alginate, whereas pectin primarily served as colonization scaffold, illuminates substrate-driven dynamics within mixed polysaccharide pools.
33876546	10	41	theme	colonization	1618:1629	arg1	scaffold					1631:1638	colonization scaffold	1618:1638	colonization scaffold	1618:1638	The bacterial preference for alginate, whereas pectin primarily served as colonization scaffold, illuminates substrate-driven dynamics within mixed polysaccharide pools.
33876546	11	42	theme	macroalgae-rich	1822:1836	arg1	habitats					1838:1845	macroalgae-rich habitats	1822:1845	macroalgae-rich habitats	1822:1845	These insights expand our understanding of bacterial niche specialization and the biological carbon pump in macroalgae-rich habitats.
33876546	3	43	theme	pectin	538:543	arg1	particles					545:553	pectin particles	538:553	pectin particles	538:553	Here, we studied the composition, functional diversity and gene expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	8	44	theme	particle-derived	1252:1267	arg1	oligomers					1269:1277	particle-derived oligomers	1252:1277	particle-derived oligomers	1252:1277	One specific Glaciecola ASV dominated the free-living fraction, possibly persisting on particle-derived oligomers through different glycoside hydrolases.
33876546	9	45	theme	Elevated	1319:1326	arg1	uptake					1337:1342	Elevated ammonium uptake	1319:1342	Elevated ammonium uptake	1319:1342	Elevated ammonium uptake and metabolism signified nitrogen as an important factor for degrading carbon-rich particles, whereas elevated methylcitrate and glyoxylate cycles suggested nutrient limitation in surrounding waters.
33876546	3	46	theme	alginate	525:532	arg1	mix					518:520	a mix	516:520	a mix of alginate and pectin particles	516:553	Here, we studied the composition, functional diversity and gene expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	2	47	theme	polysaccharide	312:325	arg1	composition					327:337	different polysaccharide composition	302:337	different polysaccharide composition	302:337	However, substrate-specific bacterial dynamics in mixtures of particle types with different polysaccharide composition, as likely occurring in natural habitats, are undescribed.
33876546	8	48	theme	glycoside	1297:1305	arg1	hydrolases					1307:1316	different glycoside hydrolases	1287:1316	different glycoside hydrolases	1287:1316	One specific Glaciecola ASV dominated the free-living fraction, possibly persisting on particle-derived oligomers through different glycoside hydrolases.
33876546	6	49	theme	diverse	985:991	arg1	lyases					1002:1007	diverse alginate lyases	985:1007	diverse alginate lyases	985:1007	Corresponding metagenome-assembled genomes (MAGs) expressed diverse alginate lyases, several colocalized in polysaccharide utilization loci.
33876546	11	50	theme	niche	1767:1771	arg1	specialization					1773:1786	bacterial niche specialization	1757:1786	bacterial niche specialization	1757:1786	These insights expand our understanding of bacterial niche specialization and the biological carbon pump in macroalgae-rich habitats.
33876546	5	51	theme	variants	848:855	arg1	predominance					814:825	predominance	814:825	predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas	814:922	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	0	52	theme	pectin	113:118	arg1	particles					120:128	pectin particles	113:128	pectin particles	113:128	Sweet spheres: succession and CAZyme expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	11	53	theme	biological	1796:1805	arg1	pump					1814:1817	the biological carbon pump	1792:1817	the biological carbon pump in macroalgae-rich habitats	1792:1845	These insights expand our understanding of bacterial niche specialization and the biological carbon pump in macroalgae-rich habitats.
33876546	1	54	theme	marine	203:208	arg1	bacteria					210:217	marine bacteria	203:217	marine bacteria	203:217	Polysaccharide particles are important substrates and microhabitats for marine bacteria.
33876546	5	55	from	predominance	814:825	arg1	Psychromonas					911:922	Psychromonas	911:922	Psychromonas	911:922	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	5	55	from	predominance	814:825	arg1	Psychrobium					895:905	Psychrobium	895:905	Psychrobium	895:905	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	5	55	from	predominance	814:825	arg1	Colwellia					884:892	Colwellia	884:892	Colwellia	884:892	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	6	56	theme	utilization	1048:1058	arg1	loci					1060:1063	polysaccharide utilization loci	1033:1063	polysaccharide utilization loci	1033:1063	Corresponding metagenome-assembled genomes (MAGs) expressed diverse alginate lyases, several colocalized in polysaccharide utilization loci.
33876546	7	57	theme	enzymes	1156:1162	arg1	abundances					1125:1134	elevated transcript abundances	1105:1134	elevated transcript abundances of pectin-degrading enzymes	1105:1162	Only a single, low-abundant MAG showed elevated transcript abundances of pectin-degrading enzymes.
33876546	2	58	theme	substrate-specific	229:246	arg1	dynamics					258:265	substrate-specific bacterial dynamics	229:265	substrate-specific bacterial dynamics in mixtures of particle types with different polysaccharide composition	229:337	However, substrate-specific bacterial dynamics in mixtures of particle types with different polysaccharide composition, as likely occurring in natural habitats, are undescribed.
33876546	2	59	theme	particle	282:289	arg1	types					291:295	particle types	282:295	particle types with different polysaccharide composition	282:337	However, substrate-specific bacterial dynamics in mixtures of particle types with different polysaccharide composition, as likely occurring in natural habitats, are undescribed.
33876546	8	60	link	particle-derived	1252:1267	arg1	oligomers					1269:1277	particle-derived oligomers	1252:1277	particle-derived oligomers	1252:1277	One specific Glaciecola ASV dominated the free-living fraction, possibly persisting on particle-derived oligomers through different glycoside hydrolases.
33876546	0	61	theme	bacterial	58:66	arg1	communities					68:78	marine bacterial communities	51:78	marine bacterial communities colonizing a mix of alginate and pectin particles	51:128	Sweet spheres: succession and CAZyme expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	1	62	theme	Polysaccharide	131:144	arg1	particles					146:154	Polysaccharide particles	131:154	Polysaccharide particles	131:154	Polysaccharide particles are important substrates and microhabitats for marine bacteria.
33876546	1	62	theme	Polysaccharide	131:144	arg1	substrates					170:179	substrates	170:179	substrates	170:179	Polysaccharide particles are important substrates and microhabitats for marine bacteria.
33876546	5	63	theme	sequence	839:846	arg1	ASVs					858:861	ASVs	858:861	ASVs	858:861	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	5	63	theme	sequence	839:846	arg1	variants					848:855	amplicon sequence variants	830:855	amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas	830:922	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	9	64	theme	methylcitrate	1455:1467	arg1	cycles					1484:1489	elevated methylcitrate and glyoxylate cycles	1446:1489	elevated methylcitrate and glyoxylate cycles	1446:1489	Elevated ammonium uptake and metabolism signified nitrogen as an important factor for degrading carbon-rich particles, whereas elevated methylcitrate and glyoxylate cycles suggested nutrient limitation in surrounding waters.
33876546	4	65	dep	alginate	639:646	arg1	particles					659:667	particles	659:667	particles	659:667	Amplicon, metagenome and metatranscriptome sequencing revealed that communities on alginate and pectin particles significantly differed from their free-living counterparts.
33876546	9	66	theme	glyoxylate	1473:1482	arg1	cycles					1484:1489	elevated methylcitrate and glyoxylate cycles	1446:1489	elevated methylcitrate and glyoxylate cycles	1446:1489	Elevated ammonium uptake and metabolism signified nitrogen as an important factor for degrading carbon-rich particles, whereas elevated methylcitrate and glyoxylate cycles suggested nutrient limitation in surrounding waters.
33876546	0	67	theme	alginate	100:107	arg1	particles					120:128	pectin particles	113:128	pectin particles	113:128	Sweet spheres: succession and CAZyme expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	0	67	theme	alginate	100:107	arg1	mix					93:95	a mix	91:95	a mix of alginate	91:107	Sweet spheres: succession and CAZyme expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	8	68	theme	specific	1169:1176	arg1	ASV					1189:1191	One specific Glaciecola ASV	1165:1191	One specific Glaciecola ASV	1165:1191	One specific Glaciecola ASV dominated the free-living fraction, possibly persisting on particle-derived oligomers through different glycoside hydrolases.
33876546	11	69	from	understanding	1740:1752	arg1	habitats					1838:1845	macroalgae-rich habitats	1822:1845	macroalgae-rich habitats	1822:1845	These insights expand our understanding of bacterial niche specialization and the biological carbon pump in macroalgae-rich habitats.
33876546	3	70	dep	composition	419:429	arg1	the					415:417	the	415:417	the	415:417	Here, we studied the composition, functional diversity and gene expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	2	71	dep	undescribed	385:395	arg1	occurring					350:358	occurring	350:358	occurring in natural habitats	350:378	However, substrate-specific bacterial dynamics in mixtures of particle types with different polysaccharide composition, as likely occurring in natural habitats, are undescribed.
33876546	4	72	from	communities	624:634	arg1	pectin					652:657	pectin	652:657	pectin	652:657	Amplicon, metagenome and metatranscriptome sequencing revealed that communities on alginate and pectin particles significantly differed from their free-living counterparts.
33876546	4	72	from	communities	624:634	arg1	alginate					639:646	alginate	639:646	alginate	639:646	Amplicon, metagenome and metatranscriptome sequencing revealed that communities on alginate and pectin particles significantly differed from their free-living counterparts.
33876546	4	73	theme	metatranscriptome	581:597	arg1	sequencing					599:608	metatranscriptome sequencing	581:608	metatranscriptome sequencing	581:608	Amplicon, metagenome and metatranscriptome sequencing revealed that communities on alginate and pectin particles significantly differed from their free-living counterparts.
33876546	7	74	dep	single	1073:1078	arg1	low-abundant					1081:1092	low-abundant	1081:1092	low-abundant	1081:1092	Only a single, low-abundant MAG showed elevated transcript abundances of pectin-degrading enzymes.
33876546	9	75	theme	surrounding	1524:1534	arg1	waters					1536:1541	surrounding waters	1524:1541	surrounding waters	1524:1541	Elevated ammonium uptake and metabolism signified nitrogen as an important factor for degrading carbon-rich particles, whereas elevated methylcitrate and glyoxylate cycles suggested nutrient limitation in surrounding waters.
33876546	3	76	theme	communities	493:503	arg1	composition					419:429	composition	419:429	composition	419:429	Here, we studied the composition, functional diversity and gene expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	3	76	theme	communities	493:503	arg1	diversity					443:451	functional diversity	432:451	functional diversity	432:451	Here, we studied the composition, functional diversity and gene expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	3	76	theme	communities	493:503	arg1	expression					462:471	gene expression	457:471	gene expression	457:471	Here, we studied the composition, functional diversity and gene expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	0	77	theme	CAZyme	30:35	arg1	expression					37:46	CAZyme expression	30:46	CAZyme expression	30:46	Sweet spheres: succession and CAZyme expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	0	77	theme	CAZyme	30:35	arg1	spheres					6:12	Sweet spheres	0:12	Sweet spheres: succession and CAZyme expression of marine bacterial communities colonizing a mix of alginate and pectin particles.	0:129	Sweet spheres: succession and CAZyme expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	3	78	theme	functional	432:441	arg1	diversity					443:451	functional diversity	432:451	functional diversity	432:451	Here, we studied the composition, functional diversity and gene expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	3	79	theme	marine	476:481	arg1	communities					493:503	marine bacterial communities	476:503	marine bacterial communities colonizing a mix of alginate and pectin particles	476:553	Here, we studied the composition, functional diversity and gene expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	6	80	theme	Corresponding	925:937	arg1	MAGs					969:972	MAGs	969:972	MAGs	969:972	Corresponding metagenome-assembled genomes (MAGs) expressed diverse alginate lyases, several colocalized in polysaccharide utilization loci.
33876546	6	80	theme	Corresponding	925:937	arg1	genomes					960:966	Corresponding metagenome-assembled genomes	925:966	Corresponding metagenome-assembled genomes (MAGs)	925:973	Corresponding metagenome-assembled genomes (MAGs) expressed diverse alginate lyases, several colocalized in polysaccharide utilization loci.
33876546	10	81	theme	mixed	1686:1690	arg1	pools					1707:1711	mixed polysaccharide pools	1686:1711	mixed polysaccharide pools	1686:1711	The bacterial preference for alginate, whereas pectin primarily served as colonization scaffold, illuminates substrate-driven dynamics within mixed polysaccharide pools.
33876546	2	82	theme	natural	363:369	arg1	habitats					371:378	natural habitats	363:378	natural habitats	363:378	However, substrate-specific bacterial dynamics in mixtures of particle types with different polysaccharide composition, as likely occurring in natural habitats, are undescribed.
33876546	7	83	theme	transcript	1114:1123	arg1	abundances					1125:1134	elevated transcript abundances	1105:1134	elevated transcript abundances of pectin-degrading enzymes	1105:1162	Only a single, low-abundant MAG showed elevated transcript abundances of pectin-degrading enzymes.
33876546	0	84	dep	spheres	6:12	arg1	succession					15:24	succession	15:24	succession	15:24	Sweet spheres: succession and CAZyme expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	0	84	dep	spheres	6:12	arg1	expression					37:46	CAZyme expression	30:46	CAZyme expression	30:46	Sweet spheres: succession and CAZyme expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	0	84	dep	spheres	6:12	arg1	spheres					6:12	Sweet spheres	0:12	Sweet spheres: succession and CAZyme expression of marine bacterial communities colonizing a mix of alginate and pectin particles.	0:129	Sweet spheres: succession and CAZyme expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	10	85	theme	bacterial	1548:1556	arg1	preference					1558:1567	The bacterial preference	1544:1567	The bacterial preference for alginate	1544:1580	The bacterial preference for alginate, whereas pectin primarily served as colonization scaffold, illuminates substrate-driven dynamics within mixed polysaccharide pools.
33876546	3	86	theme	particles	545:553	arg1	mix					518:520	a mix	516:520	a mix of alginate and pectin particles	516:553	Here, we studied the composition, functional diversity and gene expression of marine bacterial communities colonizing a mix of alginate and pectin particles.
33876546	5	87	from	dynamics	753:760	arg1	alginate					765:772	alginate	765:772	alginate	765:772	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33876546	5	87	from	dynamics	753:760	arg1	particles					785:793	pectin particles	778:793	pectin particles	778:793	Unexpectedly, microbial dynamics on alginate and pectin particles were similar, with predominance of amplicon sequence variants (ASVs) from Tenacibaculum, Colwellia, Psychrobium and Psychromonas.
33426835	9	0	theme	%	1644:1644	arg1	medlar					1646:1651	1.14% medlar	1640:1651	1.14% medlar	1640:1651	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	0	theme	%	1644:1644	arg1	grains					1617:1622	71.4% barley grains	1604:1622	71.4% barley grains	1604:1622	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	6	1	theme	one-factor-at-a-time	840:859	arg1	experimentation					861:875	the one-factor-at-a-time experimentation	836:875	the one-factor-at-a-time experimentation	836:875	Based on the one-factor-at-a-time experimentation, a quadratic regression model with the polysaccharide contents as the response value was established by central composite design (CCD).
33426835	3	2	theme	barley	525:530	arg1	substrates					552:561	substrates	552:561	substrates on solid-state fermentation (SSF) of G. lucidum	552:609	In the present study, barley grains were used as substrates on solid-state fermentation (SSF) of G. lucidum.
33426835	3	2	theme	barley	525:530	arg1	grains					532:537	barley grains	525:537	barley grains	525:537	In the present study, barley grains were used as substrates on solid-state fermentation (SSF) of G. lucidum.
33426835	6	3	theme	response	947:954	arg1	value					956:960	the response value	943:960	the response value	943:960	Based on the one-factor-at-a-time experimentation, a quadratic regression model with the polysaccharide contents as the response value was established by central composite design (CCD).
33426835	7	4	dep	%	1070:1070	arg1	peptone					1072:1078	peptone	1072:1078	2.38% peptone	1066:1078	The results showed that the predicted variables were 2.38% peptone, 1.14% medlar, and 0.25% KH2PO4 for the maximum yield of predicted polysaccharides of 11.64% in the fermented substrate.
33426835	9	5	theme	%	1658:1658	arg1	KH2PO4					1660:1665	0.25% KH2PO4	1654:1665	0.25% KH2PO4	1654:1665	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	5	theme	%	1658:1658	arg1	grains					1617:1622	71.4% barley grains	1604:1622	71.4% barley grains	1604:1622	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	8	6	theme	predicted	1253:1261	arg1	composition					1277:1287	the predicted optimum media composition	1249:1287	the predicted optimum media composition	1249:1287	The experimental polysaccharides obtained using the predicted optimum media composition constituted 11.61% of the fermented substrate, which validates the high degree of accuracy of optimization for enhanced production of polysaccharides by SSF.
33426835	1	7	theme	Ganoderma	192:200	arg1	lucidum					202:208	Ganoderma lucidum	192:208	Ganoderma lucidum	192:208	Ganoderma lucidum is a widely used medicinal mushroom in traditional Chinese medicine that creates a diverse set of bioactive compounds.
33426835	1	7	theme	Ganoderma	192:200	arg1	mushroom					237:244	a widely used medicinal mushroom	213:244	a widely used medicinal mushroom in traditional Chinese medicine that creates a diverse set of bioactive compounds	213:326	Ganoderma lucidum is a widely used medicinal mushroom in traditional Chinese medicine that creates a diverse set of bioactive compounds.
33426835	6	8	theme	polysaccharide	916:929	arg1	contents					931:938	the polysaccharide contents	912:938	the polysaccharide contents as the response value	912:960	Based on the one-factor-at-a-time experimentation, a quadratic regression model with the polysaccharide contents as the response value was established by central composite design (CCD).
33426835	8	9	theme	media	1271:1275	arg1	composition					1277:1287	the predicted optimum media composition	1249:1287	the predicted optimum media composition	1249:1287	The experimental polysaccharides obtained using the predicted optimum media composition constituted 11.61% of the fermented substrate, which validates the high degree of accuracy of optimization for enhanced production of polysaccharides by SSF.
33426835	7	10	theme	0.25	1099:1102	arg1	%					1103:1103	%	1103:1103	%	1103:1103	The results showed that the predicted variables were 2.38% peptone, 1.14% medlar, and 0.25% KH2PO4 for the maximum yield of predicted polysaccharides of 11.64% in the fermented substrate.
33426835	2	11	theme	Highland	329:336	arg1	crop					375:378	a typical nutrition-balanced crop	346:378	a typical nutrition-balanced crop	346:378	Highland barley, a typical nutrition-balanced crop, is not convenient for direct consumption but its nutritional characteristics meet the modern requirements of health food.
33426835	2	11	theme	Highland	329:336	arg1	barley					338:343	Highland barley	329:343	Highland barley	329:343	Highland barley, a typical nutrition-balanced crop, is not convenient for direct consumption but its nutritional characteristics meet the modern requirements of health food.
33426835	9	12	theme	%	1671:1671	arg1	glucose					1673:1679	2.5% glucose	1668:1679	2.5% glucose	1668:1679	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	12	theme	%	1671:1671	arg1	grains					1617:1622	71.4% barley grains	1604:1622	71.4% barley grains	1604:1622	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	4	13	theme	composition	646:656	arg1	optimization					624:635	Statistical optimization	612:635	Statistical optimization of media composition	612:656	Statistical optimization of media composition was employed for enhancing the production of polysaccharides.
33426835	7	14	from	substrate	1190:1198	arg1	polysaccharides					1147:1161	predicted polysaccharides	1137:1161	predicted polysaccharides of 11.64% in the fermented substrate	1137:1198	The results showed that the predicted variables were 2.38% peptone, 1.14% medlar, and 0.25% KH2PO4 for the maximum yield of predicted polysaccharides of 11.64% in the fermented substrate.
33426835	0	15	theme	Solid-State	137:147	arg1	Fermentation					149:160	Solid-State Fermentation	137:160	Solid-State Fermentation Using Highland Barley Grains	137:189	Statistical Optimization of Polysaccharides Production by the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) in Solid-State Fermentation Using Highland Barley Grains.
33426835	3	16	theme	lucidum	603:609	arg1	fermentation					578:589	solid-state fermentation	566:589	solid-state fermentation (SSF) of G. lucidum	566:609	In the present study, barley grains were used as substrates on solid-state fermentation (SSF) of G. lucidum.
33426835	3	16	theme	lucidum	603:609	arg1	SSF					592:594	SSF	592:594	SSF	592:594	In the present study, barley grains were used as substrates on solid-state fermentation (SSF) of G. lucidum.
33426835	1	17	theme	medicinal	227:235	arg1	lucidum					202:208	Ganoderma lucidum	192:208	Ganoderma lucidum	192:208	Ganoderma lucidum is a widely used medicinal mushroom in traditional Chinese medicine that creates a diverse set of bioactive compounds.
33426835	1	17	theme	medicinal	227:235	arg1	mushroom					237:244	a widely used medicinal mushroom	213:244	a widely used medicinal mushroom in traditional Chinese medicine that creates a diverse set of bioactive compounds	213:326	Ganoderma lucidum is a widely used medicinal mushroom in traditional Chinese medicine that creates a diverse set of bioactive compounds.
33426835	6	18	theme	composite	989:997	arg1	CCD					1007:1009	CCD	1007:1009	CCD	1007:1009	Based on the one-factor-at-a-time experimentation, a quadratic regression model with the polysaccharide contents as the response value was established by central composite design (CCD).
33426835	6	18	theme	composite	989:997	arg1	design					999:1004	central composite design	981:1004	central composite design (CCD)	981:1010	Based on the one-factor-at-a-time experimentation, a quadratic regression model with the polysaccharide contents as the response value was established by central composite design (CCD).
33426835	9	19	theme	%	1686:1686	arg1	grains					1617:1622	71.4% barley grains	1604:1622	71.4% barley grains	1604:1622	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	19	theme	%	1686:1686	arg1	7H2O					1696:1699	0.25% MgSO4 · 7H2O	1682:1699	0.25% MgSO4 · 7H2O	1682:1699	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	20	theme	·	1694:1694	arg1	grains					1617:1622	71.4% barley grains	1604:1622	71.4% barley grains	1604:1622	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	20	theme	·	1694:1694	arg1	7H2O					1696:1699	0.25% MgSO4 · 7H2O	1682:1699	0.25% MgSO4 · 7H2O	1682:1699	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	8	21	theme	substrate	1325:1333	arg1	substrate					1325:1333	the fermented substrate	1311:1333	the fermented substrate	1311:1333	The experimental polysaccharides obtained using the predicted optimum media composition constituted 11.61% of the fermented substrate, which validates the high degree of accuracy of optimization for enhanced production of polysaccharides by SSF.
33426835	8	21	theme	substrate	1325:1333	arg1	%					1306:1306	11.61%	1301:1306	11.61% of the fermented substrate, which validates the high degree of accuracy of optimization for enhanced production of polysaccharides by SSF	1301:1444	The experimental polysaccharides obtained using the predicted optimum media composition constituted 11.61% of the fermented substrate, which validates the high degree of accuracy of optimization for enhanced production of polysaccharides by SSF.
33426835	0	22	theme	Barley	177:182	arg1	Grains					184:189	Highland Barley Grains	168:189	Highland Barley Grains	168:189	Statistical Optimization of Polysaccharides Production by the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) in Solid-State Fermentation Using Highland Barley Grains.
33426835	10	23	theme	lucidum	1755:1761	arg1	00679					1763:1767	the strain G. lucidum 00679	1741:1767	the strain G. lucidum 00679	1741:1767	According to this study, the strain G. lucidum 00679 showed a good fermentation property by optimizing the media.
33426835	4	24	theme	polysaccharides	703:717	arg1	production					689:698	the production	685:698	the production of polysaccharides	685:717	Statistical optimization of media composition was employed for enhancing the production of polysaccharides.
33426835	10	25	theme	strain	1745:1750	arg1	lucidum					1755:1761	the strain G. lucidum	1741:1761	the strain G. lucidum 00679	1741:1767	According to this study, the strain G. lucidum 00679 showed a good fermentation property by optimizing the media.
33426835	9	26	theme	polysaccharides	1536:1550	arg1	substrate					1582:1590	the optimized SSF substrate	1564:1590	the optimized SSF substrate	1564:1590	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	26	theme	polysaccharides	1536:1550	arg1	production					1552:1561	polysaccharides production	1536:1561	polysaccharides production	1536:1561	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	27	theme	1	1706:1706	arg1	%					1707:1707	%	1707:1707	%	1707:1707	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	0	28	theme	Medicinal	80:88	arg1	lucidum					109:115	Medicinal Mushroom Ganoderma lucidum	80:115	Medicinal Mushroom Ganoderma lucidum	80:115	Statistical Optimization of Polysaccharides Production by the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) in Solid-State Fermentation Using Highland Barley Grains.
33426835	0	29	theme	Ganoderma	99:107	arg1	lucidum					109:115	Medicinal Mushroom Ganoderma lucidum	80:115	Medicinal Mushroom Ganoderma lucidum	80:115	Statistical Optimization of Polysaccharides Production by the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) in Solid-State Fermentation Using Highland Barley Grains.
33426835	9	30	theme	optimized	1568:1576	arg1	substrate					1582:1590	the optimized SSF substrate	1564:1590	the optimized SSF substrate	1564:1590	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	30	theme	optimized	1568:1576	arg1	production					1552:1561	polysaccharides production	1536:1561	polysaccharides production	1536:1561	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	7	31	from	%	1171:1171	arg1	substrate					1190:1198	the fermented substrate	1176:1198	the fermented substrate	1176:1198	The results showed that the predicted variables were 2.38% peptone, 1.14% medlar, and 0.25% KH2PO4 for the maximum yield of predicted polysaccharides of 11.64% in the fermented substrate.
33426835	11	32	theme	industrial	1854:1863	arg1	It					1830:1831	It	1830:1831	It	1830:1831	It might be a candidate industrial strain for further process optimization and scale-up study.
33426835	11	32	theme	industrial	1854:1863	arg1	strain					1865:1870	a candidate industrial strain	1842:1870	a candidate industrial strain for further process optimization and scale-up study	1842:1922	It might be a candidate industrial strain for further process optimization and scale-up study.
33426835	8	33	theme	accuracy	1371:1378	arg1	degree					1361:1366	the high degree	1352:1366	the high degree of accuracy of optimization for enhanced production of polysaccharides by SSF	1352:1444	The experimental polysaccharides obtained using the predicted optimum media composition constituted 11.61% of the fermented substrate, which validates the high degree of accuracy of optimization for enhanced production of polysaccharides by SSF.
33426835	8	34	theme	optimization	1383:1394	arg1	accuracy					1371:1378	accuracy	1371:1378	accuracy of optimization for enhanced production of polysaccharides by SSF	1371:1444	The experimental polysaccharides obtained using the predicted optimum media composition constituted 11.61% of the fermented substrate, which validates the high degree of accuracy of optimization for enhanced production of polysaccharides by SSF.
33426835	0	35	theme	Statistical	0:10	arg1	Optimization					12:23	Statistical Optimization	0:23	Statistical Optimization of Polysaccharides Production by the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) in Solid-State Fermentation Using Highland Barley Grains.	0:190	Statistical Optimization of Polysaccharides Production by the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) in Solid-State Fermentation Using Highland Barley Grains.
33426835	1	36	theme	diverse	293:299	arg1	set					301:303	a diverse set	291:303	a diverse set of bioactive compounds	291:326	Ganoderma lucidum is a widely used medicinal mushroom in traditional Chinese medicine that creates a diverse set of bioactive compounds.
33426835	1	36	theme	diverse	293:299	arg1	compounds					318:326	bioactive compounds	308:326	bioactive compounds	308:326	Ganoderma lucidum is a widely used medicinal mushroom in traditional Chinese medicine that creates a diverse set of bioactive compounds.
33426835	8	37	theme	enhanced	1400:1407	arg1	production					1409:1418	enhanced production	1400:1418	enhanced production of polysaccharides by SSF	1400:1444	The experimental polysaccharides obtained using the predicted optimum media composition constituted 11.61% of the fermented substrate, which validates the high degree of accuracy of optimization for enhanced production of polysaccharides by SSF.
33426835	0	38	dep	Lingzhi	62:68	arg1	lucidum					109:115	Medicinal Mushroom Ganoderma lucidum	80:115	Medicinal Mushroom Ganoderma lucidum	80:115	Statistical Optimization of Polysaccharides Production by the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) in Solid-State Fermentation Using Highland Barley Grains.
33426835	0	38	dep	Lingzhi	62:68	arg1	the					58:60	the	58:60	the	58:60	Statistical Optimization of Polysaccharides Production by the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) in Solid-State Fermentation Using Highland Barley Grains.
33426835	11	39	theme	candidate	1844:1852	arg1	It					1830:1831	It	1830:1831	It	1830:1831	It might be a candidate industrial strain for further process optimization and scale-up study.
33426835	11	39	theme	candidate	1844:1852	arg1	strain					1865:1870	a candidate industrial strain	1842:1870	a candidate industrial strain for further process optimization and scale-up study	1842:1922	It might be a candidate industrial strain for further process optimization and scale-up study.
33426835	7	40	theme	%	1171:1171	arg1	polysaccharides					1147:1161	predicted polysaccharides	1137:1161	predicted polysaccharides of 11.64% in the fermented substrate	1137:1198	The results showed that the predicted variables were 2.38% peptone, 1.14% medlar, and 0.25% KH2PO4 for the maximum yield of predicted polysaccharides of 11.64% in the fermented substrate.
33426835	9	41	theme	%	1608:1608	arg1	medlar					1646:1651	1.14% medlar	1640:1651	1.14% medlar	1640:1651	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	41	theme	%	1608:1608	arg1	CaCO3					1709:1713	1% CaCO3	1706:1713	1% CaCO3	1706:1713	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	41	theme	%	1608:1608	arg1	7H2O					1696:1699	0.25% MgSO4 · 7H2O	1682:1699	0.25% MgSO4 · 7H2O	1682:1699	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	41	theme	%	1608:1608	arg1	glucose					1673:1679	2.5% glucose	1668:1679	2.5% glucose	1668:1679	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	41	theme	%	1608:1608	arg1	peptone					1631:1637	2.38% peptone	1625:1637	2.38% peptone	1625:1637	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	41	theme	%	1608:1608	arg1	KH2PO4					1660:1665	0.25% KH2PO4	1654:1665	0.25% KH2PO4	1654:1665	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	41	theme	%	1608:1608	arg1	grains					1617:1622	71.4% barley grains	1604:1622	71.4% barley grains	1604:1622	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	10	42	theme	good	1778:1781	arg1	property					1796:1803	a good fermentation property	1776:1803	a good fermentation property	1776:1803	According to this study, the strain G. lucidum 00679 showed a good fermentation property by optimizing the media.
33426835	0	43	theme	Production	44:53	arg1	Optimization					12:23	Statistical Optimization	0:23	Statistical Optimization of Polysaccharides Production by the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) in Solid-State Fermentation Using Highland Barley Grains.	0:190	Statistical Optimization of Polysaccharides Production by the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) in Solid-State Fermentation Using Highland Barley Grains.
33426835	1	44	theme	compounds	318:326	arg1	set					301:303	a diverse set	291:303	a diverse set of bioactive compounds	291:326	Ganoderma lucidum is a widely used medicinal mushroom in traditional Chinese medicine that creates a diverse set of bioactive compounds.
33426835	1	44	theme	compounds	318:326	arg1	compounds					318:326	bioactive compounds	308:326	bioactive compounds	308:326	Ganoderma lucidum is a widely used medicinal mushroom in traditional Chinese medicine that creates a diverse set of bioactive compounds.
33426835	2	45	theme	health	490:495	arg1	food					497:500	health food	490:500	health food	490:500	Highland barley, a typical nutrition-balanced crop, is not convenient for direct consumption but its nutritional characteristics meet the modern requirements of health food.
33426835	3	46	theme	present	510:516	arg1	study					518:522	the present study	506:522	the present study	506:522	In the present study, barley grains were used as substrates on solid-state fermentation (SSF) of G. lucidum.
33426835	7	47	theme	predicted	1137:1145	arg1	polysaccharides					1147:1161	predicted polysaccharides	1137:1161	predicted polysaccharides of 11.64% in the fermented substrate	1137:1198	The results showed that the predicted variables were 2.38% peptone, 1.14% medlar, and 0.25% KH2PO4 for the maximum yield of predicted polysaccharides of 11.64% in the fermented substrate.
33426835	6	48	theme	quadratic	880:888	arg1	model					901:905	a quadratic regression model	878:905	a quadratic regression model with the polysaccharide contents as the response value	878:960	Based on the one-factor-at-a-time experimentation, a quadratic regression model with the polysaccharide contents as the response value was established by central composite design (CCD).
33426835	9	49	theme	2.38	1625:1628	arg1	%					1629:1629	%	1629:1629	%	1629:1629	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	1	50	theme	traditional	249:259	arg1	medicine					269:276	traditional Chinese medicine	249:276	traditional Chinese medicine	249:276	Ganoderma lucidum is a widely used medicinal mushroom in traditional Chinese medicine that creates a diverse set of bioactive compounds.
33426835	9	51	theme	1.14	1640:1643	arg1	%					1644:1644	%	1644:1644	%	1644:1644	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	5	52	theme	important	776:784	arg1	medlar					729:734	medlar	729:734	medlar	729:734	Peptone, medlar, and KH2PO4 were identified as the most important components for producing polysaccharide.
33426835	5	52	theme	important	776:784	arg1	KH2PO4					741:746	KH2PO4	741:746	KH2PO4	741:746	Peptone, medlar, and KH2PO4 were identified as the most important components for producing polysaccharide.
33426835	5	52	theme	important	776:784	arg1	Peptone					720:726	Peptone	720:726	Peptone	720:726	Peptone, medlar, and KH2PO4 were identified as the most important components for producing polysaccharide.
33426835	5	52	theme	important	776:784	arg1	components					786:795	the most important components	767:795	the most important components for producing polysaccharide	767:824	Peptone, medlar, and KH2PO4 were identified as the most important components for producing polysaccharide.
33426835	7	53	theme	maximum	1120:1126	arg1	yield					1128:1132	the maximum yield	1116:1132	the maximum yield of predicted polysaccharides of 11.64% in the fermented substrate	1116:1198	The results showed that the predicted variables were 2.38% peptone, 1.14% medlar, and 0.25% KH2PO4 for the maximum yield of predicted polysaccharides of 11.64% in the fermented substrate.
33426835	9	54	theme	0.25	1654:1657	arg1	%					1658:1658	%	1658:1658	%	1658:1658	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	2	55	theme	nutrition-balanced	356:373	arg1	crop					375:378	a typical nutrition-balanced crop	346:378	a typical nutrition-balanced crop	346:378	Highland barley, a typical nutrition-balanced crop, is not convenient for direct consumption but its nutritional characteristics meet the modern requirements of health food.
33426835	2	55	theme	nutrition-balanced	356:373	arg1	barley					338:343	Highland barley	329:343	Highland barley	329:343	Highland barley, a typical nutrition-balanced crop, is not convenient for direct consumption but its nutritional characteristics meet the modern requirements of health food.
33426835	7	56	theme	%	1103:1103	arg1	KH2PO4					1105:1110	0.25% KH2PO4	1099:1110	0.25% KH2PO4	1099:1110	The results showed that the predicted variables were 2.38% peptone, 1.14% medlar, and 0.25% KH2PO4 for the maximum yield of predicted polysaccharides of 11.64% in the fermented substrate.
33426835	3	57	used	used	544:547	arg2	grains					532:537	barley grains	525:537	barley grains	525:537	In the present study, barley grains were used as substrates on solid-state fermentation (SSF) of G. lucidum.
33426835	3	57	used	used	544:547	arg2	substrates					552:561	substrates	552:561	substrates on solid-state fermentation (SSF) of G. lucidum	552:609	In the present study, barley grains were used as substrates on solid-state fermentation (SSF) of G. lucidum.
33426835	9	58	theme	2.5	1668:1670	arg1	%					1671:1671	%	1671:1671	%	1671:1671	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	8	59	theme	optimum	1263:1269	arg1	composition					1277:1287	the predicted optimum media composition	1249:1287	the predicted optimum media composition	1249:1287	The experimental polysaccharides obtained using the predicted optimum media composition constituted 11.61% of the fermented substrate, which validates the high degree of accuracy of optimization for enhanced production of polysaccharides by SSF.
33426835	7	60	theme	medlar	1087:1092	arg1	%					1085:1085	1.14% medlar	1081:1092	1.14% medlar	1081:1092	The results showed that the predicted variables were 2.38% peptone, 1.14% medlar, and 0.25% KH2PO4 for the maximum yield of predicted polysaccharides of 11.64% in the fermented substrate.
33426835	3	61	theme	solid-state	566:576	arg1	fermentation					578:589	solid-state fermentation	566:589	solid-state fermentation (SSF) of G. lucidum	566:609	In the present study, barley grains were used as substrates on solid-state fermentation (SSF) of G. lucidum.
33426835	3	61	theme	solid-state	566:576	arg1	SSF					592:594	SSF	592:594	SSF	592:594	In the present study, barley grains were used as substrates on solid-state fermentation (SSF) of G. lucidum.
33426835	4	62	theme	media	640:644	arg1	composition					646:656	media composition	640:656	media composition	640:656	Statistical optimization of media composition was employed for enhancing the production of polysaccharides.
33426835	3	63	theme	G.	600:601	arg1	lucidum					603:609	G. lucidum	600:609	G. lucidum	600:609	In the present study, barley grains were used as substrates on solid-state fermentation (SSF) of G. lucidum.
33426835	1	64	theme	used	222:225	arg1	lucidum					202:208	Ganoderma lucidum	192:208	Ganoderma lucidum	192:208	Ganoderma lucidum is a widely used medicinal mushroom in traditional Chinese medicine that creates a diverse set of bioactive compounds.
33426835	1	64	theme	used	222:225	arg1	mushroom					237:244	a widely used medicinal mushroom	213:244	a widely used medicinal mushroom in traditional Chinese medicine that creates a diverse set of bioactive compounds	213:326	Ganoderma lucidum is a widely used medicinal mushroom in traditional Chinese medicine that creates a diverse set of bioactive compounds.
33426835	8	65	theme	experimental	1205:1216	arg1	polysaccharides					1218:1232	The experimental polysaccharides	1201:1232	The experimental polysaccharides obtained using the predicted optimum media composition	1201:1287	The experimental polysaccharides obtained using the predicted optimum media composition constituted 11.61% of the fermented substrate, which validates the high degree of accuracy of optimization for enhanced production of polysaccharides by SSF.
33426835	9	66	theme	0.25	1682:1685	arg1	%					1686:1686	%	1686:1686	%	1686:1686	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	0	67	theme	Highland	168:175	arg1	Grains					184:189	Highland Barley Grains	168:189	Highland Barley Grains	168:189	Statistical Optimization of Polysaccharides Production by the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) in Solid-State Fermentation Using Highland Barley Grains.
33426835	6	68	theme	central	981:987	arg1	CCD					1007:1009	CCD	1007:1009	CCD	1007:1009	Based on the one-factor-at-a-time experimentation, a quadratic regression model with the polysaccharide contents as the response value was established by central composite design (CCD).
33426835	6	68	theme	central	981:987	arg1	design					999:1004	central composite design	981:1004	central composite design (CCD)	981:1010	Based on the one-factor-at-a-time experimentation, a quadratic regression model with the polysaccharide contents as the response value was established by central composite design (CCD).
33426835	9	69	theme	MgSO4	1688:1692	arg1	grains					1617:1622	71.4% barley grains	1604:1622	71.4% barley grains	1604:1622	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	69	theme	MgSO4	1688:1692	arg1	7H2O					1696:1699	0.25% MgSO4 · 7H2O	1682:1699	0.25% MgSO4 · 7H2O	1682:1699	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	1	70	from	mushroom	237:244	arg1	medicine					269:276	traditional Chinese medicine	249:276	traditional Chinese medicine	249:276	Ganoderma lucidum is a widely used medicinal mushroom in traditional Chinese medicine that creates a diverse set of bioactive compounds.
33426835	11	71	theme	scale-up	1909:1916	arg1	study					1918:1922	scale-up study	1909:1922	scale-up study	1909:1922	It might be a candidate industrial strain for further process optimization and scale-up study.
33426835	7	72	theme	predicted	1041:1049	arg1	variables					1051:1059	the predicted variables	1037:1059	the predicted variables	1037:1059	The results showed that the predicted variables were 2.38% peptone, 1.14% medlar, and 0.25% KH2PO4 for the maximum yield of predicted polysaccharides of 11.64% in the fermented substrate.
33426835	7	72	theme	predicted	1041:1049	arg1	%					1070:1070	2.38%	1066:1070	2.38% peptone	1066:1078	The results showed that the predicted variables were 2.38% peptone, 1.14% medlar, and 0.25% KH2PO4 for the maximum yield of predicted polysaccharides of 11.64% in the fermented substrate.
33426835	9	73	theme	G.	1521:1522	arg1	lucidum					1524:1530	G. lucidum	1521:1530	G. lucidum	1521:1530	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	8	74	theme	fermented	1315:1323	arg1	substrate					1325:1333	the fermented substrate	1311:1333	the fermented substrate	1311:1333	The experimental polysaccharides obtained using the predicted optimum media composition constituted 11.61% of the fermented substrate, which validates the high degree of accuracy of optimization for enhanced production of polysaccharides by SSF.
33426835	10	75	theme	G.	1752:1753	arg1	lucidum					1755:1761	the strain G. lucidum	1741:1761	the strain G. lucidum 00679	1741:1767	According to this study, the strain G. lucidum 00679 showed a good fermentation property by optimizing the media.
33426835	0	76	theme	Mushroom	90:97	arg1	lucidum					109:115	Medicinal Mushroom Ganoderma lucidum	80:115	Medicinal Mushroom Ganoderma lucidum	80:115	Statistical Optimization of Polysaccharides Production by the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) in Solid-State Fermentation Using Highland Barley Grains.
33426835	9	77	theme	%	1707:1707	arg1	grains					1617:1622	71.4% barley grains	1604:1622	71.4% barley grains	1604:1622	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	77	theme	%	1707:1707	arg1	CaCO3					1709:1713	1% CaCO3	1706:1713	1% CaCO3	1706:1713	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	7	78	from	polysaccharides	1147:1161	arg1	substrate					1190:1198	the fermented substrate	1176:1198	the fermented substrate	1176:1198	The results showed that the predicted variables were 2.38% peptone, 1.14% medlar, and 0.25% KH2PO4 for the maximum yield of predicted polysaccharides of 11.64% in the fermented substrate.
33426835	3	79	from	substrates	552:561	arg1	fermentation					578:589	solid-state fermentation	566:589	solid-state fermentation (SSF) of G. lucidum	566:609	In the present study, barley grains were used as substrates on solid-state fermentation (SSF) of G. lucidum.
33426835	3	79	from	substrates	552:561	arg1	SSF					592:594	SSF	592:594	SSF	592:594	In the present study, barley grains were used as substrates on solid-state fermentation (SSF) of G. lucidum.
33426835	8	80	theme	high	1356:1359	arg1	degree					1361:1366	the high degree	1352:1366	the high degree of accuracy of optimization for enhanced production of polysaccharides by SSF	1352:1444	The experimental polysaccharides obtained using the predicted optimum media composition constituted 11.61% of the fermented substrate, which validates the high degree of accuracy of optimization for enhanced production of polysaccharides by SSF.
33426835	7	81	theme	fermented	1180:1188	arg1	substrate					1190:1198	the fermented substrate	1176:1198	the fermented substrate	1176:1198	The results showed that the predicted variables were 2.38% peptone, 1.14% medlar, and 0.25% KH2PO4 for the maximum yield of predicted polysaccharides of 11.64% in the fermented substrate.
33426835	9	82	theme	SSF	1578:1580	arg1	substrate					1582:1590	the optimized SSF substrate	1564:1590	the optimized SSF substrate	1564:1590	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	82	theme	SSF	1578:1580	arg1	production					1552:1561	polysaccharides production	1536:1561	polysaccharides production	1536:1561	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	6	83	with	model	901:905	arg1	contents					931:938	the polysaccharide contents	912:938	the polysaccharide contents as the response value	912:960	Based on the one-factor-at-a-time experimentation, a quadratic regression model with the polysaccharide contents as the response value was established by central composite design (CCD).
33426835	2	84	theme	direct	403:408	arg1	consumption					410:420	direct consumption	403:420	direct consumption	403:420	Highland barley, a typical nutrition-balanced crop, is not convenient for direct consumption but its nutritional characteristics meet the modern requirements of health food.
33426835	2	85	theme	modern	467:472	arg1	requirements					474:485	the modern requirements	463:485	the modern requirements of health food	463:500	Highland barley, a typical nutrition-balanced crop, is not convenient for direct consumption but its nutritional characteristics meet the modern requirements of health food.
33426835	0	86	theme	Polysaccharides	28:42	arg1	Production					44:53	Polysaccharides Production	28:53	Polysaccharides Production	28:53	Statistical Optimization of Polysaccharides Production by the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) in Solid-State Fermentation Using Highland Barley Grains.
33426835	9	87	theme	71.4	1604:1607	arg1	%					1608:1608	%	1608:1608	%	1608:1608	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	11	88	theme	further	1876:1882	arg1	optimization					1892:1903	further process optimization	1876:1903	further process optimization	1876:1903	It might be a candidate industrial strain for further process optimization and scale-up study.
33426835	10	89	theme	fermentation	1783:1794	arg1	property					1796:1803	a good fermentation property	1776:1803	a good fermentation property	1776:1803	According to this study, the strain G. lucidum 00679 showed a good fermentation property by optimizing the media.
33426835	2	90	theme	nutritional	430:440	arg1	characteristics					442:456	its nutritional characteristics	426:456	its nutritional characteristics	426:456	Highland barley, a typical nutrition-balanced crop, is not convenient for direct consumption but its nutritional characteristics meet the modern requirements of health food.
33426835	7	91	theme	polysaccharides	1147:1161	arg1	yield					1128:1132	the maximum yield	1116:1132	the maximum yield of predicted polysaccharides of 11.64% in the fermented substrate	1116:1198	The results showed that the predicted variables were 2.38% peptone, 1.14% medlar, and 0.25% KH2PO4 for the maximum yield of predicted polysaccharides of 11.64% in the fermented substrate.
33426835	1	92	theme	bioactive	308:316	arg1	compounds					318:326	bioactive compounds	308:326	bioactive compounds	308:326	Ganoderma lucidum is a widely used medicinal mushroom in traditional Chinese medicine that creates a diverse set of bioactive compounds.
33426835	9	93	theme	barley	1491:1496	arg1	grains					1498:1503	barley grains	1491:1503	barley grains	1491:1503	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	94	theme	barley	1610:1615	arg1	medlar					1646:1651	1.14% medlar	1640:1651	1.14% medlar	1640:1651	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	94	theme	barley	1610:1615	arg1	CaCO3					1709:1713	1% CaCO3	1706:1713	1% CaCO3	1706:1713	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	94	theme	barley	1610:1615	arg1	7H2O					1696:1699	0.25% MgSO4 · 7H2O	1682:1699	0.25% MgSO4 · 7H2O	1682:1699	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	94	theme	barley	1610:1615	arg1	glucose					1673:1679	2.5% glucose	1668:1679	2.5% glucose	1668:1679	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	94	theme	barley	1610:1615	arg1	peptone					1631:1637	2.38% peptone	1625:1637	2.38% peptone	1625:1637	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	94	theme	barley	1610:1615	arg1	KH2PO4					1660:1665	0.25% KH2PO4	1654:1665	0.25% KH2PO4	1654:1665	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	94	theme	barley	1610:1615	arg1	grains					1617:1622	71.4% barley grains	1604:1622	71.4% barley grains	1604:1622	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	2	95	theme	food	497:500	arg1	requirements					474:485	the modern requirements	463:485	the modern requirements of health food	463:500	Highland barley, a typical nutrition-balanced crop, is not convenient for direct consumption but its nutritional characteristics meet the modern requirements of health food.
33426835	6	96	theme	regression	890:899	arg1	model					901:905	a quadratic regression model	878:905	a quadratic regression model with the polysaccharide contents as the response value	878:960	Based on the one-factor-at-a-time experimentation, a quadratic regression model with the polysaccharide contents as the response value was established by central composite design (CCD).
33426835	8	97	theme	polysaccharides	1423:1437	arg1	production					1409:1418	enhanced production	1400:1418	enhanced production of polysaccharides by SSF	1400:1444	The experimental polysaccharides obtained using the predicted optimum media composition constituted 11.61% of the fermented substrate, which validates the high degree of accuracy of optimization for enhanced production of polysaccharides by SSF.
33426835	2	98	theme	typical	348:354	arg1	crop					375:378	a typical nutrition-balanced crop	346:378	a typical nutrition-balanced crop	346:378	Highland barley, a typical nutrition-balanced crop, is not convenient for direct consumption but its nutritional characteristics meet the modern requirements of health food.
33426835	2	98	theme	typical	348:354	arg1	barley					338:343	Highland barley	329:343	Highland barley	329:343	Highland barley, a typical nutrition-balanced crop, is not convenient for direct consumption but its nutritional characteristics meet the modern requirements of health food.
33426835	9	99	theme	%	1629:1629	arg1	peptone					1631:1637	2.38% peptone	1625:1637	2.38% peptone	1625:1637	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	9	99	theme	%	1629:1629	arg1	grains					1617:1622	71.4% barley grains	1604:1622	71.4% barley grains	1604:1622	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	11	100	theme	process	1884:1890	arg1	optimization					1892:1903	further process optimization	1876:1903	further process optimization	1876:1903	It might be a candidate industrial strain for further process optimization and scale-up study.
33426835	4	101	theme	Statistical	612:622	arg1	optimization					624:635	Statistical optimization	612:635	Statistical optimization of media composition	612:656	Statistical optimization of media composition was employed for enhancing the production of polysaccharides.
33426835	9	102	theme	grains	1498:1503	arg1	process					1480:1486	the process	1476:1486	the process of barley grains	1476:1503	This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3.
33426835	1	103	theme	Chinese	261:267	arg1	medicine					269:276	traditional Chinese medicine	249:276	traditional Chinese medicine	249:276	Ganoderma lucidum is a widely used medicinal mushroom in traditional Chinese medicine that creates a diverse set of bioactive compounds.
34882526	9	0	theme	theoretical	1595:1605	arg1	basis					1607:1611	a theoretical basis	1593:1611	a theoretical basis for recycling manure and improving biogas engineering in large-scale pig farms	1593:1690	These findings can provide a theoretical basis for recycling manure and improving biogas engineering in large-scale pig farms.
34882526	8	1	contain	had	1322:1324	arg1	pH					1288:1289	The pH	1284:1289	The pH	1284:1289	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	8	1	contain	had	1322:1324	arg1	As					1319:1320	As	1319:1320	As	1319:1320	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	8	1	contain	had	1322:1324	arg1	T1					1312:1313	T1	1312:1313	T1	1312:1313	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	8	1	contain	had	1322:1324	arg2	effect					1340:1345	a significant effect	1326:1345	a significant effect on the microbial community structure	1326:1382	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	8	1	contain	had	1322:1324	arg1	contents					1292:1299	contents	1292:1299	contents of lignin	1292:1309	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	8	2	theme	Pb	1473:1474	arg1	contents					1421:1428	the contents	1417:1428	the contents of total phosphorus, NO3--N, cellulose and Pb	1417:1474	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	6	3	theme	%	1179:1179	arg1	abundance					1156:1164	a relative abundance	1145:1164	a relative abundance of 73.6%-99.4%	1145:1179	Bacteroidota, Firmicutes, Proteobacteria and Spirochaetota dominated in the pig manure and digestate samples, with a relative abundance of 73.6%-99.4%.
34882526	1	4	theme	regional	168:175	arg1	characteristics					177:191	regional characteristics	168:191	regional characteristics	168:191	Livestock and poultry breeding modes, feed compositions and manure collection systems have regional characteristics, which can directly affect the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products.
34882526	8	5	theme	digestate	1547:1555	arg1	samples					1557:1563	the digestate samples	1543:1563	the digestate samples	1543:1563	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	6	6	theme	digestate	1121:1129	arg1	samples					1131:1137	the pig manure and digestate samples	1102:1137	samples	1131:1137	Bacteroidota, Firmicutes, Proteobacteria and Spirochaetota dominated in the pig manure and digestate samples, with a relative abundance of 73.6%-99.4%.
34882526	8	7	theme	community	1364:1372	arg1	structure					1374:1382	the microbial community structure	1350:1382	the microbial community structure	1350:1382	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	5	8	theme	mg	890:891	arg1	kg-1					893:896	16.09-31.22 mg kg-1	878:896	16.09-31.22 mg kg-1	878:896	The As content was 16.09-31.22 mg kg-1 in the pig manure and digestate samples, which exceeded the standard limitation requirements in fertilizers in China (≤15 mg kg-1).
34882526	5	8	theme	mg	890:891	arg1	content					866:872	The As content	859:872	The As content	859:872	The As content was 16.09-31.22 mg kg-1 in the pig manure and digestate samples, which exceeded the standard limitation requirements in fertilizers in China (≤15 mg kg-1).
34882526	5	9	theme	16.09-31.22	878:888	arg1	mg					890:891	mg	890:891	mg	890:891	The As content was 16.09-31.22 mg kg-1 in the pig manure and digestate samples, which exceeded the standard limitation requirements in fertilizers in China (≤15 mg kg-1).
34882526	5	10	theme	As	863:864	arg1	kg-1					893:896	16.09-31.22 mg kg-1	878:896	16.09-31.22 mg kg-1	878:896	The As content was 16.09-31.22 mg kg-1 in the pig manure and digestate samples, which exceeded the standard limitation requirements in fertilizers in China (≤15 mg kg-1).
34882526	5	10	theme	As	863:864	arg1	content					866:872	The As content	859:872	The As content	859:872	The As content was 16.09-31.22 mg kg-1 in the pig manure and digestate samples, which exceeded the standard limitation requirements in fertilizers in China (≤15 mg kg-1).
34882526	2	11	from	farms	489:493	arg1	Quzhou					516:521	Quzhou	516:521	Quzhou	516:521	The chemical, heavy metal contents and microbiological characteristics of pig manures and digestates were characterized in five pig farms and biogas plants in Quzhou (Zhejiang Province) in this study.
34882526	2	11	from	farms	489:493	arg1	Province					533:540	Province	533:540	Province	533:540	The chemical, heavy metal contents and microbiological characteristics of pig manures and digestates were characterized in five pig farms and biogas plants in Quzhou (Zhejiang Province) in this study.
34882526	6	12	theme	%	1173:1173	arg1	%					1179:1179	73.6%-99.4%	1169:1179	73.6%-99.4%	1169:1179	Bacteroidota, Firmicutes, Proteobacteria and Spirochaetota dominated in the pig manure and digestate samples, with a relative abundance of 73.6%-99.4%.
34882526	8	13	from	effect	1340:1345	arg1	structure					1374:1382	the microbial community structure	1350:1382	the microbial community structure	1350:1382	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	1	14	theme	energy	278:283	arg1	production					285:294	energy production	278:294	energy production by anaerobic digestion	278:317	Livestock and poultry breeding modes, feed compositions and manure collection systems have regional characteristics, which can directly affect the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products.
34882526	9	15	theme	biogas	1648:1653	arg1	engineering					1655:1665	biogas engineering	1648:1665	biogas engineering in large-scale pig farms	1648:1690	These findings can provide a theoretical basis for recycling manure and improving biogas engineering in large-scale pig farms.
34882526	1	16	contain	have	163:166	arg2	characteristics					177:191	regional characteristics	168:191	regional characteristics	168:191	Livestock and poultry breeding modes, feed compositions and manure collection systems have regional characteristics, which can directly affect the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products.
34882526	1	16	contain	have	163:166	arg1	modes					108:112	Livestock and poultry breeding modes	77:112	Livestock and poultry breeding modes	77:112	Livestock and poultry breeding modes, feed compositions and manure collection systems have regional characteristics, which can directly affect the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products.
34882526	1	16	contain	have	163:166	arg1	compositions					120:131	feed compositions	115:131	feed compositions	115:131	Livestock and poultry breeding modes, feed compositions and manure collection systems have regional characteristics, which can directly affect the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products.
34882526	1	16	contain	have	163:166	arg1	systems					155:161	manure collection systems	137:161	manure collection systems	137:161	Livestock and poultry breeding modes, feed compositions and manure collection systems have regional characteristics, which can directly affect the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products.
34882526	1	17	theme	Livestock	77:85	arg1	modes					108:112	Livestock and poultry breeding modes	77:112	Livestock and poultry breeding modes	77:112	Livestock and poultry breeding modes, feed compositions and manure collection systems have regional characteristics, which can directly affect the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products.
34882526	7	18	theme	pig	1223:1225	arg1	samples					1234:1240	the pig manure samples	1219:1240	the pig manure samples	1219:1240	The microbial community structure in the pig manure samples was quite different among the five farms.
34882526	5	19	from	fertilizers	994:1004	arg1	kg-1					1023:1026	≤15 mg kg-1	1016:1026	≤15 mg kg-1	1016:1026	The As content was 16.09-31.22 mg kg-1 in the pig manure and digestate samples, which exceeded the standard limitation requirements in fertilizers in China (≤15 mg kg-1).
34882526	5	19	from	fertilizers	994:1004	arg1	China					1009:1013	China	1009:1013	China (≤15 mg kg-1)	1009:1027	The As content was 16.09-31.22 mg kg-1 in the pig manure and digestate samples, which exceeded the standard limitation requirements in fertilizers in China (≤15 mg kg-1).
34882526	5	20	theme	mg	1020:1021	arg1	kg-1					1023:1026	≤15 mg kg-1	1016:1026	≤15 mg kg-1	1016:1026	The As content was 16.09-31.22 mg kg-1 in the pig manure and digestate samples, which exceeded the standard limitation requirements in fertilizers in China (≤15 mg kg-1).
34882526	5	20	theme	mg	1020:1021	arg1	China					1009:1013	China	1009:1013	China (≤15 mg kg-1)	1009:1027	The As content was 16.09-31.22 mg kg-1 in the pig manure and digestate samples, which exceeded the standard limitation requirements in fertilizers in China (≤15 mg kg-1).
34882526	2	21	theme	biogas	499:504	arg1	plants					506:511	biogas plants	499:511	biogas plants	499:511	The chemical, heavy metal contents and microbiological characteristics of pig manures and digestates were characterized in five pig farms and biogas plants in Quzhou (Zhejiang Province) in this study.
34882526	9	22	theme	large-scale	1670:1680	arg1	farms					1686:1690	large-scale pig farms	1670:1690	large-scale pig farms	1670:1690	These findings can provide a theoretical basis for recycling manure and improving biogas engineering in large-scale pig farms.
34882526	7	23	theme	manure	1227:1232	arg1	samples					1234:1240	the pig manure samples	1219:1240	the pig manure samples	1219:1240	The microbial community structure in the pig manure samples was quite different among the five farms.
34882526	8	24	theme	pig	1391:1393	arg1	samples					1402:1408	the pig manure samples	1387:1408	the pig manure samples	1387:1408	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	1	25	theme	poultry	91:97	arg1	modes					108:112	Livestock and poultry breeding modes	77:112	Livestock and poultry breeding modes	77:112	Livestock and poultry breeding modes, feed compositions and manure collection systems have regional characteristics, which can directly affect the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products.
34882526	2	26	theme	microbiological	396:410	arg1	characteristics					412:426	microbiological characteristics	396:426	microbiological characteristics	396:426	The chemical, heavy metal contents and microbiological characteristics of pig manures and digestates were characterized in five pig farms and biogas plants in Quzhou (Zhejiang Province) in this study.
34882526	0	27	theme	Chemical	0:7	arg1	characterization					29:44	Chemical and microbiological characterization	0:44	Chemical and microbiological characterization of pig	0:51	Chemical and microbiological characterization of pig manures and digestates.
34882526	6	28	theme	relative	1147:1154	arg1	abundance					1156:1164	a relative abundance	1145:1164	a relative abundance of 73.6%-99.4%	1145:1179	Bacteroidota, Firmicutes, Proteobacteria and Spirochaetota dominated in the pig manure and digestate samples, with a relative abundance of 73.6%-99.4%.
34882526	1	29	theme	breeding	99:106	arg1	modes					108:112	Livestock and poultry breeding modes	77:112	Livestock and poultry breeding modes	77:112	Livestock and poultry breeding modes, feed compositions and manure collection systems have regional characteristics, which can directly affect the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products.
34882526	9	30	from	engineering	1655:1665	arg1	farms					1686:1690	large-scale pig farms	1670:1690	large-scale pig farms	1670:1690	These findings can provide a theoretical basis for recycling manure and improving biogas engineering in large-scale pig farms.
34882526	9	31	theme	pig	1682:1684	arg1	farms					1686:1690	large-scale pig farms	1670:1690	large-scale pig farms	1670:1690	These findings can provide a theoretical basis for recycling manure and improving biogas engineering in large-scale pig farms.
34882526	2	32	dep	chemical	361:368	arg1	heavy					371:375	heavy	371:375	heavy	371:375	The chemical, heavy metal contents and microbiological characteristics of pig manures and digestates were characterized in five pig farms and biogas plants in Quzhou (Zhejiang Province) in this study.
34882526	2	33	theme	digestates	447:456	arg1	characteristics					412:426	microbiological characteristics	396:426	microbiological characteristics	396:426	The chemical, heavy metal contents and microbiological characteristics of pig manures and digestates were characterized in five pig farms and biogas plants in Quzhou (Zhejiang Province) in this study.
34882526	2	33	theme	digestates	447:456	arg1	contents					383:390	metal contents	377:390	metal contents	377:390	The chemical, heavy metal contents and microbiological characteristics of pig manures and digestates were characterized in five pig farms and biogas plants in Quzhou (Zhejiang Province) in this study.
34882526	0	34	theme	microbiological	13:27	arg1	characterization					29:44	Chemical and microbiological characterization	0:44	Chemical and microbiological characterization of pig	0:51	Chemical and microbiological characterization of pig manures and digestates.
34882526	1	35	theme	anaerobic	299:307	arg1	digestion					309:317	anaerobic digestion	299:317	anaerobic digestion	299:317	Livestock and poultry breeding modes, feed compositions and manure collection systems have regional characteristics, which can directly affect the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products.
34882526	2	36	dep	contents	383:390	arg1	The					357:359	The	357:359	The	357:359	The chemical, heavy metal contents and microbiological characteristics of pig manures and digestates were characterized in five pig farms and biogas plants in Quzhou (Zhejiang Province) in this study.
34882526	3	37	theme	anaerobic	653:661	arg1	digestion					663:671	anaerobic digestion	653:671	anaerobic digestion	653:671	The results showed that hemicellulose and cellulose of pig manures could be partly degraded in anaerobic digestion, but lignin was difficultly degraded and accumulated in digestates.
34882526	4	38	theme	Zn	756:757	arg1	content					745:751	The content	741:751	The content of Zn	741:757	The content of Zn was highest in the pig manure and digestate samples, followed by Cu, Cr, As, Ni, Pb, Cd, T1 and Hg.
34882526	4	38	theme	Zn	756:757	arg1	highest					763:769	highest	763:769	highest	763:769	The content of Zn was highest in the pig manure and digestate samples, followed by Cu, Cr, As, Ni, Pb, Cd, T1 and Hg.
34882526	0	39	theme	pig	49:51	arg1	characterization					29:44	Chemical and microbiological characterization	0:44	Chemical and microbiological characterization of pig	0:51	Chemical and microbiological characterization of pig manures and digestates.
34882526	7	40	theme	microbial	1186:1194	arg1	structure					1206:1214	The microbial community structure	1182:1214	The microbial community structure in the pig manure samples	1182:1240	The microbial community structure in the pig manure samples was quite different among the five farms.
34882526	7	40	theme	microbial	1186:1194	arg1	different					1252:1260	different	1252:1260	different	1252:1260	The microbial community structure in the pig manure samples was quite different among the five farms.
34882526	2	41	from	plants	506:511	arg1	Quzhou					516:521	Quzhou	516:521	Quzhou	516:521	The chemical, heavy metal contents and microbiological characteristics of pig manures and digestates were characterized in five pig farms and biogas plants in Quzhou (Zhejiang Province) in this study.
34882526	2	41	from	plants	506:511	arg1	Province					533:540	Province	533:540	Province	533:540	The chemical, heavy metal contents and microbiological characteristics of pig manures and digestates were characterized in five pig farms and biogas plants in Quzhou (Zhejiang Province) in this study.
34882526	6	42	theme	pig	1106:1108	arg1	manure					1110:1115	the pig manure and digestate samples	1102:1137	manure	1110:1115	Bacteroidota, Firmicutes, Proteobacteria and Spirochaetota dominated in the pig manure and digestate samples, with a relative abundance of 73.6%-99.4%.
34882526	2	43	theme	manures	435:441	arg1	characteristics					412:426	microbiological characteristics	396:426	microbiological characteristics	396:426	The chemical, heavy metal contents and microbiological characteristics of pig manures and digestates were characterized in five pig farms and biogas plants in Quzhou (Zhejiang Province) in this study.
34882526	2	43	theme	manures	435:441	arg1	contents					383:390	metal contents	377:390	metal contents	377:390	The chemical, heavy metal contents and microbiological characteristics of pig manures and digestates were characterized in five pig farms and biogas plants in Quzhou (Zhejiang Province) in this study.
34882526	3	44	theme	pig	613:615	arg1	manures					617:623	pig manures	613:623	pig manures	613:623	The results showed that hemicellulose and cellulose of pig manures could be partly degraded in anaerobic digestion, but lignin was difficultly degraded and accumulated in digestates.
34882526	2	45	theme	pig	431:433	arg1	manures					435:441	pig manures	431:441	pig manures	431:441	The chemical, heavy metal contents and microbiological characteristics of pig manures and digestates were characterized in five pig farms and biogas plants in Quzhou (Zhejiang Province) in this study.
34882526	1	46	theme	resource	323:330	arg1	utilization					332:342	resource utilization	323:342	resource utilization of products	323:354	Livestock and poultry breeding modes, feed compositions and manure collection systems have regional characteristics, which can directly affect the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products.
34882526	2	47	theme	chemical	361:368	arg1	contents					383:390	metal contents	377:390	metal contents	377:390	The chemical, heavy metal contents and microbiological characteristics of pig manures and digestates were characterized in five pig farms and biogas plants in Quzhou (Zhejiang Province) in this study.
34882526	8	48	theme	manure	1395:1400	arg1	samples					1402:1408	the pig manure samples	1387:1408	the pig manure samples	1387:1408	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	1	49	theme	feed	115:118	arg1	compositions					120:131	feed compositions	115:131	feed compositions	115:131	Livestock and poultry breeding modes, feed compositions and manure collection systems have regional characteristics, which can directly affect the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products.
34882526	2	50	theme	pig	485:487	arg1	farms					489:493	five pig farms	480:493	five pig farms	480:493	The chemical, heavy metal contents and microbiological characteristics of pig manures and digestates were characterized in five pig farms and biogas plants in Quzhou (Zhejiang Province) in this study.
34882526	8	51	theme	phosphorus	1439:1448	arg1	contents					1421:1428	the contents	1417:1428	the contents of total phosphorus, NO3--N, cellulose and Pb	1417:1474	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	8	52	theme	microbial	1354:1362	arg1	structure					1374:1382	the microbial community structure	1350:1382	the microbial community structure	1350:1382	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	8	53	theme	significant	1328:1338	arg1	effect					1340:1345	a significant effect	1326:1345	a significant effect on the microbial community structure	1326:1382	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	8	54	theme	lignin	1304:1309	arg1	pH					1288:1289	The pH	1284:1289	The pH	1284:1289	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	8	54	theme	lignin	1304:1309	arg1	T1					1312:1313	T1	1312:1313	T1	1312:1313	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	8	54	theme	lignin	1304:1309	arg1	contents					1292:1299	contents	1292:1299	contents of lignin	1292:1309	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	8	55	theme	total	1433:1437	arg1	phosphorus					1439:1448	total phosphorus	1433:1448	total phosphorus	1433:1448	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	5	56	theme	digestate	920:928	arg1	samples					930:936	the pig manure and digestate samples	901:936	samples	930:936	The As content was 16.09-31.22 mg kg-1 in the pig manure and digestate samples, which exceeded the standard limitation requirements in fertilizers in China (≤15 mg kg-1).
34882526	4	57	dep	highest	763:769	arg1	followed					812:819	followed	812:819	followed by Cu, Cr, As, Ni, Pb, Cd, T1 and Hg	812:856	The content of Zn was highest in the pig manure and digestate samples, followed by Cu, Cr, As, Ni, Pb, Cd, T1 and Hg.
34882526	5	58	theme	standard	958:965	arg1	requirements					978:989	the standard limitation requirements	954:989	the standard limitation requirements in fertilizers in China (≤15 mg kg-1)	954:1027	The As content was 16.09-31.22 mg kg-1 in the pig manure and digestate samples, which exceeded the standard limitation requirements in fertilizers in China (≤15 mg kg-1).
34882526	5	59	theme	pig	905:907	arg1	manure					909:914	the pig manure and digestate samples	901:936	manure	909:914	The As content was 16.09-31.22 mg kg-1 in the pig manure and digestate samples, which exceeded the standard limitation requirements in fertilizers in China (≤15 mg kg-1).
34882526	7	60	from	structure	1206:1214	arg1	samples					1234:1240	the pig manure samples	1219:1240	the pig manure samples	1219:1240	The microbial community structure in the pig manure samples was quite different among the five farms.
34882526	8	61	theme	NO3--N	1451:1456	arg1	contents					1421:1428	the contents	1417:1428	the contents of total phosphorus, NO3--N, cellulose and Pb	1417:1474	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	4	62	theme	pig	778:780	arg1	manure					782:787	the pig manure and digestate samples	774:809	manure	782:787	The content of Zn was highest in the pig manure and digestate samples, followed by Cu, Cr, As, Ni, Pb, Cd, T1 and Hg.
34882526	5	63	theme	limitation	967:976	arg1	requirements					978:989	the standard limitation requirements	954:989	the standard limitation requirements in fertilizers in China (≤15 mg kg-1)	954:1027	The As content was 16.09-31.22 mg kg-1 in the pig manure and digestate samples, which exceeded the standard limitation requirements in fertilizers in China (≤15 mg kg-1).
34882526	1	64	theme	manure	137:142	arg1	systems					155:161	manure collection systems	137:161	manure collection systems	137:161	Livestock and poultry breeding modes, feed compositions and manure collection systems have regional characteristics, which can directly affect the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products.
34882526	8	65	theme	community	1520:1528	arg1	structure					1530:1538	the microbial community structure	1506:1538	the microbial community structure	1506:1538	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	1	66	theme	livestock	239:247	arg1	composition					224:234	the composition	220:234	the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products	220:354	Livestock and poultry breeding modes, feed compositions and manure collection systems have regional characteristics, which can directly affect the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products.
34882526	5	67	from	kg-1	893:896	arg1	manure					909:914	the pig manure and digestate samples	901:936	manure	909:914	The As content was 16.09-31.22 mg kg-1 in the pig manure and digestate samples, which exceeded the standard limitation requirements in fertilizers in China (≤15 mg kg-1).
34882526	5	67	from	kg-1	893:896	arg1	samples					930:936	the pig manure and digestate samples	901:936	samples	930:936	The As content was 16.09-31.22 mg kg-1 in the pig manure and digestate samples, which exceeded the standard limitation requirements in fertilizers in China (≤15 mg kg-1).
34882526	3	68	theme	manures	617:623	arg1	cellulose					600:608	cellulose	600:608	cellulose	600:608	The results showed that hemicellulose and cellulose of pig manures could be partly degraded in anaerobic digestion, but lignin was difficultly degraded and accumulated in digestates.
34882526	3	68	theme	manures	617:623	arg1	hemicellulose					582:594	hemicellulose	582:594	hemicellulose	582:594	The results showed that hemicellulose and cellulose of pig manures could be partly degraded in anaerobic digestion, but lignin was difficultly degraded and accumulated in digestates.
34882526	7	69	theme	community	1196:1204	arg1	structure					1206:1214	The microbial community structure	1182:1214	The microbial community structure in the pig manure samples	1182:1240	The microbial community structure in the pig manure samples was quite different among the five farms.
34882526	7	69	theme	community	1196:1204	arg1	different					1252:1260	different	1252:1260	different	1252:1260	The microbial community structure in the pig manure samples was quite different among the five farms.
34882526	1	70	theme	collection	144:153	arg1	systems					155:161	manure collection systems	137:161	manure collection systems	137:161	Livestock and poultry breeding modes, feed compositions and manure collection systems have regional characteristics, which can directly affect the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products.
34882526	8	71	theme	microbial	1510:1518	arg1	structure					1530:1538	the microbial community structure	1506:1538	the microbial community structure	1506:1538	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	8	72	theme	cellulose	1459:1467	arg1	contents					1421:1428	the contents	1417:1428	the contents of total phosphorus, NO3--N, cellulose and Pb	1417:1474	The pH, contents of lignin, T1 and As had a significant effect on the microbial community structure in the pig manure samples, while the contents of total phosphorus, NO3--N, cellulose and Pb could significantly influence the microbial community structure in the digestate samples.
34882526	5	73	from	requirements	978:989	arg1	fertilizers					994:1004	fertilizers	994:1004	fertilizers in China (≤15 mg kg-1)	994:1027	The As content was 16.09-31.22 mg kg-1 in the pig manure and digestate samples, which exceeded the standard limitation requirements in fertilizers in China (≤15 mg kg-1).
34882526	1	74	theme	products	347:354	arg1	manure					270:275	manure	270:275	manure	270:275	Livestock and poultry breeding modes, feed compositions and manure collection systems have regional characteristics, which can directly affect the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products.
34882526	1	74	theme	products	347:354	arg1	production					285:294	energy production	278:294	energy production by anaerobic digestion	278:317	Livestock and poultry breeding modes, feed compositions and manure collection systems have regional characteristics, which can directly affect the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products.
34882526	1	74	theme	products	347:354	arg1	utilization					332:342	resource utilization	323:342	resource utilization of products	323:354	Livestock and poultry breeding modes, feed compositions and manure collection systems have regional characteristics, which can directly affect the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products.
34882526	4	75	theme	digestate	793:801	arg1	samples					803:809	the pig manure and digestate samples	774:809	samples	803:809	The content of Zn was highest in the pig manure and digestate samples, followed by Cu, Cr, As, Ni, Pb, Cd, T1 and Hg.
34882526	2	76	theme	metal	377:381	arg1	contents					383:390	metal contents	377:390	metal contents	377:390	The chemical, heavy metal contents and microbiological characteristics of pig manures and digestates were characterized in five pig farms and biogas plants in Quzhou (Zhejiang Province) in this study.
34882526	1	77	theme	poultry	253:259	arg1	composition					224:234	the composition	220:234	the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products	220:354	Livestock and poultry breeding modes, feed compositions and manure collection systems have regional characteristics, which can directly affect the composition of livestock and poultry breeding manure, energy production by anaerobic digestion and resource utilization of products.
34910075	6	0	theme	immediate	882:890	arg1	analysis					892:899	immediate analysis	882:899	immediate analysis	882:899	Half of the specimens from each restored group were subjected to interface aging and the remaining specimens were used for immediate analysis.
34910075	7	1	theme	interface	1174:1182	arg1	testing					1016:1022	chemical composition testing	995:1022	chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5)	995:1128	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	7	1	theme	interface	1174:1182	arg1	n=10					988:991	n=10	988:991	n=10	988:991	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	7	1	theme	interface	1174:1182	arg1	test					982:985	microtensile bond strength (µTBS) test	948:985	microtensile bond strength (µTBS) test (n=10)	948:992	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	7	1	theme	interface	1174:1182	arg1	analysis					1149:1156	morphological analysis	1135:1156	morphological analysis of the adhesive interface	1135:1182	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	13	2	theme	phosphate	1719:1727	arg1	intensity					1706:1714	the intensity	1702:1714	the intensity of phosphate and carbonate bands	1702:1747	FTIR showed a decrease in the intensity of phosphate and carbonate bands after using chitosan.
34910075	10	3	theme	dentin	1427:1432	arg1	adhesion					1434:1441	dentin adhesion	1427:1441	dentin adhesion between the adhesive systems (p=0.652)	1427:1480	No significant differences were observed in dentin adhesion between the adhesive systems (p=0.652).
34910075	3	4	with	groups	533:538	arg1	gel					590:592	2.5% chitosan gel	576:592	2.5% chitosan gel (1 min)	576:600	METHODOLOGY Enamel was removed from 80 molars and the teeth were divided into two groups: without chitosan (control) or with 2.5% chitosan gel (1 min).
34910075	3	4	with	groups	533:538	arg1	min					597:599	1 min	595:599	1 min	595:599	METHODOLOGY Enamel was removed from 80 molars and the teeth were divided into two groups: without chitosan (control) or with 2.5% chitosan gel (1 min).
34910075	6	5	theme	specimens	771:779	arg1	Half					759:762	Half	759:762	Half of the specimens from each restored group	759:804	Half of the specimens from each restored group were subjected to interface aging and the remaining specimens were used for immediate analysis.
34910075	2	6	theme	treatment	288:296	arg1	effect					271:276	the effect	267:276	the effect of dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system on improvement of bond strength and preservation of the interface durability	267:448	This study assessed the effect of dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system on improvement of bond strength and preservation of the interface durability.
34910075	3	7	attach	removed	474:480	arg1	molars					490:495	80 molars	487:495	80 molars	487:495	METHODOLOGY Enamel was removed from 80 molars and the teeth were divided into two groups: without chitosan (control) or with 2.5% chitosan gel (1 min).
34910075	3	7	attach	removed	474:480	arg2	Enamel					463:468	METHODOLOGY Enamel	451:468	METHODOLOGY Enamel	451:468	METHODOLOGY Enamel was removed from 80 molars and the teeth were divided into two groups: without chitosan (control) or with 2.5% chitosan gel (1 min).
34910075	11	8	theme	Immediate	1483:1491	arg1	µTBS					1493:1496	Immediate µTBS	1483:1496	Immediate µTBS	1483:1496	Immediate µTBS was not significantly different from that after 6 months (p=0.274).
34910075	11	9	from	that	1535:1538	arg1	different					1520:1528	different	1520:1528	different	1520:1528	Immediate µTBS was not significantly different from that after 6 months (p=0.274).
34910075	10	10	theme	adhesive	1455:1462	arg1	p=0.652					1473:1479	p=0.652	1473:1479	p=0.652	1473:1479	No significant differences were observed in dentin adhesion between the adhesive systems (p=0.652).
34910075	10	10	theme	adhesive	1455:1462	arg1	systems					1464:1470	the adhesive systems	1451:1470	the adhesive systems (p=0.652)	1451:1480	No significant differences were observed in dentin adhesion between the adhesive systems (p=0.652).
34910075	1	11	theme	collagen	201:208	arg1	hydrolysis					179:188	hydrolysis	179:188	hydrolysis of exposed collagen	179:208	OBJECTIVE Degradation of the dentin-resin interface can occur due to hydrolysis of exposed collagen, resulting in reduced bond strength.
34910075	7	12	theme	Fourier-transform	1030:1046	arg1	n=4					1078:1080	n=4	1078:1080	n=4	1078:1080	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	7	12	theme	Fourier-transform	1030:1046	arg1	spectroscopy					1064:1075	Fourier-transform infrared (FTIR) spectroscopy	1030:1075	Fourier-transform infrared (FTIR) spectroscopy (n=4)	1030:1081	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	13	13	from	decrease	1690:1697	arg1	intensity					1706:1714	the intensity	1702:1714	the intensity of phosphate and carbonate bands	1702:1747	FTIR showed a decrease in the intensity of phosphate and carbonate bands after using chitosan.
34910075	2	14	theme	bond	391:394	arg1	strength					396:403	bond strength	391:403	bond strength	391:403	This study assessed the effect of dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system on improvement of bond strength and preservation of the interface durability.
34910075	14	15	theme	Dentin	1783:1788	arg1	treatment					1790:1798	CONCLUSIONS Dentin treatment	1771:1798	CONCLUSIONS Dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system	1771:1873	CONCLUSIONS Dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system improved the immediate and preserved the 6-month bond strength of the adhesive interface.
34910075	7	16	theme	chemical	995:1002	arg1	testing					1016:1022	chemical composition testing	995:1022	chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5)	995:1128	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	3	17	theme	%	579:579	arg1	gel					590:592	2.5% chitosan gel	576:592	2.5% chitosan gel (1 min)	576:600	METHODOLOGY Enamel was removed from 80 molars and the teeth were divided into two groups: without chitosan (control) or with 2.5% chitosan gel (1 min).
34910075	3	17	theme	%	579:579	arg1	min					597:599	1 min	595:599	1 min	595:599	METHODOLOGY Enamel was removed from 80 molars and the teeth were divided into two groups: without chitosan (control) or with 2.5% chitosan gel (1 min).
34910075	7	18	theme	µTBS	976:979	arg1	n=10					988:991	n=10	988:991	n=10	988:991	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	7	18	theme	µTBS	976:979	arg1	test					982:985	microtensile bond strength (µTBS) test	948:985	microtensile bond strength (µTBS) test (n=10)	948:992	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	7	19	theme	strength	966:973	arg1	n=10					988:991	n=10	988:991	n=10	988:991	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	7	19	theme	strength	966:973	arg1	test					982:985	microtensile bond strength (µTBS) test	948:985	microtensile bond strength (µTBS) test (n=10)	948:992	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	1	20	theme	interface	152:160	arg1	Degradation					120:130	OBJECTIVE Degradation	110:130	OBJECTIVE Degradation of the dentin-resin interface	110:160	OBJECTIVE Degradation of the dentin-resin interface can occur due to hydrolysis of exposed collagen, resulting in reduced bond strength.
34910075	12	21	theme	chemical	1622:1629	arg1	composition					1646:1656	the chemical and structural composition	1618:1656	the chemical and structural composition of the specimens	1618:1673	EDS and SEM did not show significant differences in the chemical and structural composition of the specimens.
34910075	10	22	located	observed	1415:1422	arg1	adhesion					1434:1441	dentin adhesion	1427:1441	dentin adhesion between the adhesive systems (p=0.652)	1427:1480	No significant differences were observed in dentin adhesion between the adhesive systems (p=0.652).
34910075	10	22	located	observed	1415:1422	arg2	differences					1398:1408	No significant differences	1383:1408	No significant differences	1383:1408	No significant differences were observed in dentin adhesion between the adhesive systems (p=0.652).
34910075	7	23	theme	adhesive	1165:1172	arg1	interface					1174:1182	the adhesive interface	1161:1182	the adhesive interface	1161:1182	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	7	24	theme	microtensile	948:959	arg1	n=10					988:991	n=10	988:991	n=10	988:991	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	7	24	theme	microtensile	948:959	arg1	test					982:985	microtensile bond strength (µTBS) test	948:985	microtensile bond strength (µTBS) test (n=10)	948:992	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	2	25	with	treatment	288:296	arg1	chitosan					303:310	chitosan	303:310	chitosan combined with an etch-and-rinse or self-etch adhesive system	303:371	This study assessed the effect of dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system on improvement of bond strength and preservation of the interface durability.
34910075	4	26	theme	adhesive	668:675	arg1	system					677:682	the adhesive system	664:682	the adhesive system	664:682	They were further subdivided into two subgroups according to the adhesive system: etch-and-rinse or self-etch.
34910075	0	27	theme	self-etch	82:90	arg1	systems					101:107	etch-and-rinse or self-etch adhesive systems	64:107	etch-and-rinse or self-etch adhesive systems	64:107	Chitosan improves the durability of resin-dentin interface with etch-and-rinse or self-etch adhesive systems.
34910075	12	28	theme	significant	1591:1601	arg1	differences					1603:1613	significant differences	1591:1613	significant differences in the chemical and structural composition of the specimens	1591:1673	EDS and SEM did not show significant differences in the chemical and structural composition of the specimens.
34910075	7	29	theme	morphological	1135:1147	arg1	analysis					1149:1156	morphological analysis	1135:1156	morphological analysis of the adhesive interface	1135:1182	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	10	30	theme	significant	1386:1396	arg1	differences					1398:1408	No significant differences	1383:1408	No significant differences	1383:1408	No significant differences were observed in dentin adhesion between the adhesive systems (p=0.652).
34910075	2	31	theme	preservation	409:420	arg1	improvement					376:386	improvement	376:386	improvement of bond strength and preservation of the interface durability	376:448	This study assessed the effect of dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system on improvement of bond strength and preservation of the interface durability.
34910075	2	32	theme	strength	396:403	arg1	improvement					376:386	improvement	376:386	improvement of bond strength and preservation of the interface durability	376:448	This study assessed the effect of dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system on improvement of bond strength and preservation of the interface durability.
34910075	14	33	theme	6-month	1916:1922	arg1	strength					1929:1936	the 6-month bond strength	1912:1936	the 6-month bond strength of the adhesive interface	1912:1962	CONCLUSIONS Dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system improved the immediate and preserved the 6-month bond strength of the adhesive interface.
34910075	13	34	theme	carbonate	1733:1741	arg1	intensity					1706:1714	the intensity	1702:1714	the intensity of phosphate and carbonate bands	1702:1747	FTIR showed a decrease in the intensity of phosphate and carbonate bands after using chitosan.
34910075	2	35	theme	durability	439:448	arg1	preservation					409:420	preservation	409:420	preservation of the interface durability	409:448	This study assessed the effect of dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system on improvement of bond strength and preservation of the interface durability.
34910075	2	35	theme	durability	439:448	arg1	strength					396:403	bond strength	391:403	bond strength	391:403	This study assessed the effect of dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system on improvement of bond strength and preservation of the interface durability.
34910075	13	36	dep	phosphate	1719:1727	arg1	bands					1743:1747	bands	1743:1747	bands	1743:1747	FTIR showed a decrease in the intensity of phosphate and carbonate bands after using chitosan.
34910075	2	37	from	effect	271:276	arg1	improvement					376:386	improvement	376:386	improvement of bond strength and preservation of the interface durability	376:448	This study assessed the effect of dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system on improvement of bond strength and preservation of the interface durability.
34910075	14	38	theme	etch-and-rinse	1831:1844	arg1	system					1868:1873	an etch-and-rinse or self-etch adhesive system	1828:1873	an etch-and-rinse or self-etch adhesive system	1828:1873	CONCLUSIONS Dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system improved the immediate and preserved the 6-month bond strength of the adhesive interface.
34910075	14	39	with	treatment	1790:1798	arg1	chitosan					1805:1812	chitosan	1805:1812	chitosan	1805:1812	CONCLUSIONS Dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system improved the immediate and preserved the 6-month bond strength of the adhesive interface.
34910075	6	40	theme	interface	824:832	arg1	aging					834:838	interface aging	824:838	interface aging	824:838	Half of the specimens from each restored group were subjected to interface aging and the remaining specimens were used for immediate analysis.
34910075	6	41	from	Half	759:762	arg1	group					800:804	each restored group	786:804	each restored group	786:804	Half of the specimens from each restored group were subjected to interface aging and the remaining specimens were used for immediate analysis.
34910075	0	42	theme	etch-and-rinse	64:77	arg1	systems					101:107	etch-and-rinse or self-etch adhesive systems	64:107	etch-and-rinse or self-etch adhesive systems	64:107	Chitosan improves the durability of resin-dentin interface with etch-and-rinse or self-etch adhesive systems.
34910075	2	43	theme	adhesive	357:364	arg1	system					366:371	an etch-and-rinse or self-etch adhesive system	326:371	an etch-and-rinse or self-etch adhesive system	326:371	This study assessed the effect of dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system on improvement of bond strength and preservation of the interface durability.
34910075	7	44	theme	energy-dispersive	1087:1103	arg1	spectroscopy					1105:1116	energy-dispersive spectroscopy	1087:1116	energy-dispersive spectroscopy (EDS) (n=5)	1087:1128	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	7	44	theme	energy-dispersive	1087:1103	arg1	EDS					1119:1121	EDS	1119:1121	EDS	1119:1121	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	7	44	theme	energy-dispersive	1087:1103	arg1	n=5					1125:1127	n=5	1125:1127	n=5	1125:1127	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	1	45	theme	bond	232:235	arg1	strength					237:244	reduced bond strength	224:244	reduced bond strength	224:244	OBJECTIVE Degradation of the dentin-resin interface can occur due to hydrolysis of exposed collagen, resulting in reduced bond strength.
34910075	14	46	theme	adhesive	1859:1866	arg1	system					1868:1873	an etch-and-rinse or self-etch adhesive system	1828:1873	an etch-and-rinse or self-etch adhesive system	1828:1873	CONCLUSIONS Dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system improved the immediate and preserved the 6-month bond strength of the adhesive interface.
34910075	0	47	with	interface	49:57	arg1	systems					101:107	etch-and-rinse or self-etch adhesive systems	64:107	etch-and-rinse or self-etch adhesive systems	64:107	Chitosan improves the durability of resin-dentin interface with etch-and-rinse or self-etch adhesive systems.
34910075	9	48	theme	adhesive	1316:1323	arg1	interface					1325:1333	the adhesive interface	1312:1333	the adhesive interface	1312:1333	RESULTS Chitosan improved the µTBS of the adhesive interface when compared with the control group (p=0.004).
34910075	7	49	theme	electron	1199:1206	arg1	SEM					1220:1222	SEM	1220:1222	SEM	1220:1222	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	7	49	theme	electron	1199:1206	arg1	n=5					1226:1228	n=5	1226:1228	n=5	1226:1228	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	7	49	theme	electron	1199:1206	arg1	microscopy					1208:1217	scanning electron microscopy	1190:1217	scanning electron microscopy (SEM) (n=5)	1190:1229	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	12	50	theme	specimens	1665:1673	arg1	composition					1646:1656	the chemical and structural composition	1618:1656	the chemical and structural composition of the specimens	1618:1673	EDS and SEM did not show significant differences in the chemical and structural composition of the specimens.
34910075	2	51	theme	dentin	281:286	arg1	treatment					288:296	dentin treatment	281:296	dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system	281:371	This study assessed the effect of dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system on improvement of bond strength and preservation of the interface durability.
34910075	9	52	theme	control	1358:1364	arg1	p=0.004					1373:1379	p=0.004	1373:1379	p=0.004	1373:1379	RESULTS Chitosan improved the µTBS of the adhesive interface when compared with the control group (p=0.004).
34910075	9	52	theme	control	1358:1364	arg1	group					1366:1370	the control group	1354:1370	the control group (p=0.004)	1354:1380	RESULTS Chitosan improved the µTBS of the adhesive interface when compared with the control group (p=0.004).
34910075	0	53	theme	interface	49:57	arg1	durability					22:31	the durability	18:31	the durability of resin-dentin interface with etch-and-rinse or self-etch adhesive systems	18:107	Chitosan improves the durability of resin-dentin interface with etch-and-rinse or self-etch adhesive systems.
34910075	6	54	theme	remaining	848:856	arg1	specimens					858:866	the remaining specimens	844:866	the remaining specimens	844:866	Half of the specimens from each restored group were subjected to interface aging and the remaining specimens were used for immediate analysis.
34910075	12	55	theme	structural	1635:1644	arg1	composition					1646:1656	the chemical and structural composition	1618:1656	the chemical and structural composition of the specimens	1618:1673	EDS and SEM did not show significant differences in the chemical and structural composition of the specimens.
34910075	5	56	theme	composite	742:750	arg1	resin					752:756	a composite resin	740:756	a composite resin	740:756	Dentin was restored using a composite resin.
34910075	7	57	theme	infrared	1048:1055	arg1	n=4					1078:1080	n=4	1078:1080	n=4	1078:1080	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	7	57	theme	infrared	1048:1055	arg1	spectroscopy					1064:1075	Fourier-transform infrared (FTIR) spectroscopy	1030:1075	Fourier-transform infrared (FTIR) spectroscopy (n=4)	1030:1081	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	1	58	theme	exposed	193:199	arg1	collagen					201:208	exposed collagen	193:208	exposed collagen	193:208	OBJECTIVE Degradation of the dentin-resin interface can occur due to hydrolysis of exposed collagen, resulting in reduced bond strength.
34910075	7	59	dep	infrared	1048:1055	arg1	FTIR					1058:1061	FTIR	1058:1061	FTIR	1058:1061	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	7	60	theme	composition	1004:1014	arg1	testing					1016:1022	chemical composition testing	995:1022	chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5)	995:1128	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	3	61	theme	chitosan	581:588	arg1	gel					590:592	2.5% chitosan gel	576:592	2.5% chitosan gel (1 min)	576:600	METHODOLOGY Enamel was removed from 80 molars and the teeth were divided into two groups: without chitosan (control) or with 2.5% chitosan gel (1 min).
34910075	3	61	theme	chitosan	581:588	arg1	min					597:599	1 min	595:599	1 min	595:599	METHODOLOGY Enamel was removed from 80 molars and the teeth were divided into two groups: without chitosan (control) or with 2.5% chitosan gel (1 min).
34910075	3	62	theme	2.5	576:578	arg1	%					579:579	%	579:579	%	579:579	METHODOLOGY Enamel was removed from 80 molars and the teeth were divided into two groups: without chitosan (control) or with 2.5% chitosan gel (1 min).
34910075	14	63	theme	CONCLUSIONS	1771:1781	arg1	treatment					1790:1798	CONCLUSIONS Dentin treatment	1771:1798	CONCLUSIONS Dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system	1771:1873	CONCLUSIONS Dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system improved the immediate and preserved the 6-month bond strength of the adhesive interface.
34910075	12	64	from	differences	1603:1613	arg1	composition					1646:1656	the chemical and structural composition	1618:1656	the chemical and structural composition of the specimens	1618:1673	EDS and SEM did not show significant differences in the chemical and structural composition of the specimens.
34910075	1	65	theme	dentin-resin	139:150	arg1	interface					152:160	the dentin-resin interface	135:160	the dentin-resin interface	135:160	OBJECTIVE Degradation of the dentin-resin interface can occur due to hydrolysis of exposed collagen, resulting in reduced bond strength.
34910075	0	66	theme	adhesive	92:99	arg1	systems					101:107	etch-and-rinse or self-etch adhesive systems	64:107	etch-and-rinse or self-etch adhesive systems	64:107	Chitosan improves the durability of resin-dentin interface with etch-and-rinse or self-etch adhesive systems.
34910075	7	67	theme	bond	961:964	arg1	n=10					988:991	n=10	988:991	n=10	988:991	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	7	67	theme	bond	961:964	arg1	test					982:985	microtensile bond strength (µTBS) test	948:985	microtensile bond strength (µTBS) test (n=10)	948:992	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	6	68	used	used	873:876	arg2	specimens					858:866	the remaining specimens	844:866	the remaining specimens	844:866	Half of the specimens from each restored group were subjected to interface aging and the remaining specimens were used for immediate analysis.
34910075	14	69	theme	adhesive	1945:1952	arg1	interface					1954:1962	the adhesive interface	1941:1962	the adhesive interface	1941:1962	CONCLUSIONS Dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system improved the immediate and preserved the 6-month bond strength of the adhesive interface.
34910075	8	70	theme	three-way	1257:1265	arg1	ANOVA					1267:1271	three-way ANOVA	1257:1271	three-way ANOVA	1257:1271	Data were analyzed using three-way ANOVA.
34910075	6	71	from	group	800:804	arg1	Half					759:762	Half	759:762	Half of the specimens from each restored group	759:804	Half of the specimens from each restored group were subjected to interface aging and the remaining specimens were used for immediate analysis.
34910075	6	71	from	group	800:804	arg1	specimens					771:779	the specimens	767:779	the specimens from each restored group	767:804	Half of the specimens from each restored group were subjected to interface aging and the remaining specimens were used for immediate analysis.
34910075	14	72	theme	bond	1924:1927	arg1	strength					1929:1936	the 6-month bond strength	1912:1936	the 6-month bond strength of the adhesive interface	1912:1962	CONCLUSIONS Dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system improved the immediate and preserved the 6-month bond strength of the adhesive interface.
34910075	6	73	theme	restored	791:798	arg1	group					800:804	each restored group	786:804	each restored group	786:804	Half of the specimens from each restored group were subjected to interface aging and the remaining specimens were used for immediate analysis.
34910075	3	74	theme	METHODOLOGY	451:461	arg1	Enamel					463:468	METHODOLOGY Enamel	451:468	METHODOLOGY Enamel	451:468	METHODOLOGY Enamel was removed from 80 molars and the teeth were divided into two groups: without chitosan (control) or with 2.5% chitosan gel (1 min).
34910075	1	75	theme	OBJECTIVE	110:118	arg1	Degradation					120:130	OBJECTIVE Degradation	110:130	OBJECTIVE Degradation of the dentin-resin interface	110:160	OBJECTIVE Degradation of the dentin-resin interface can occur due to hydrolysis of exposed collagen, resulting in reduced bond strength.
34910075	2	76	theme	interface	429:437	arg1	durability					439:448	the interface durability	425:448	the interface durability	425:448	This study assessed the effect of dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system on improvement of bond strength and preservation of the interface durability.
34910075	0	77	theme	resin-dentin	36:47	arg1	interface					49:57	resin-dentin interface	36:57	resin-dentin interface with etch-and-rinse or self-etch adhesive systems	36:107	Chitosan improves the durability of resin-dentin interface with etch-and-rinse or self-etch adhesive systems.
34910075	9	78	theme	RESULTS	1274:1280	arg1	Chitosan					1282:1289	RESULTS Chitosan	1274:1289	RESULTS Chitosan	1274:1289	RESULTS Chitosan improved the µTBS of the adhesive interface when compared with the control group (p=0.004).
34910075	2	79	theme	self-etch	347:355	arg1	system					366:371	an etch-and-rinse or self-etch adhesive system	326:371	an etch-and-rinse or self-etch adhesive system	326:371	This study assessed the effect of dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system on improvement of bond strength and preservation of the interface durability.
34910075	4	80	dep	subgroups	641:649	arg1	self-etch					703:711	self-etch	703:711	self-etch	703:711	They were further subdivided into two subgroups according to the adhesive system: etch-and-rinse or self-etch.
34910075	4	80	dep	subgroups	641:649	arg1	etch-and-rinse					685:698	etch-and-rinse	685:698	etch-and-rinse	685:698	They were further subdivided into two subgroups according to the adhesive system: etch-and-rinse or self-etch.
34910075	4	80	dep	subgroups	641:649	arg1	subgroups					641:649	two subgroups	637:649	two subgroups according to the adhesive system: etch-and-rinse or self-etch	637:711	They were further subdivided into two subgroups according to the adhesive system: etch-and-rinse or self-etch.
34910075	14	81	theme	interface	1954:1962	arg1	strength					1929:1936	the 6-month bond strength	1912:1936	the 6-month bond strength of the adhesive interface	1912:1962	CONCLUSIONS Dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system improved the immediate and preserved the 6-month bond strength of the adhesive interface.
34910075	2	82	theme	etch-and-rinse	329:342	arg1	system					366:371	an etch-and-rinse or self-etch adhesive system	326:371	an etch-and-rinse or self-etch adhesive system	326:371	This study assessed the effect of dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system on improvement of bond strength and preservation of the interface durability.
34910075	1	83	theme	reduced	224:230	arg1	strength					237:244	reduced bond strength	224:244	reduced bond strength	224:244	OBJECTIVE Degradation of the dentin-resin interface can occur due to hydrolysis of exposed collagen, resulting in reduced bond strength.
34910075	14	84	theme	self-etch	1849:1857	arg1	system					1868:1873	an etch-and-rinse or self-etch adhesive system	1828:1873	an etch-and-rinse or self-etch adhesive system	1828:1873	CONCLUSIONS Dentin treatment with chitosan combined with an etch-and-rinse or self-etch adhesive system improved the immediate and preserved the 6-month bond strength of the adhesive interface.
34910075	7	85	theme	scanning	1190:1197	arg1	SEM					1220:1222	SEM	1220:1222	SEM	1220:1222	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	7	85	theme	scanning	1190:1197	arg1	n=5					1226:1228	n=5	1226:1228	n=5	1226:1228	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	7	85	theme	scanning	1190:1197	arg1	microscopy					1208:1217	scanning electron microscopy	1190:1217	scanning electron microscopy (SEM) (n=5)	1190:1229	The specimens were sectioned and subjected to microtensile bond strength (µTBS) test (n=10), chemical composition testing using Fourier-transform infrared (FTIR) spectroscopy (n=4) and energy-dispersive spectroscopy (EDS) (n=5), and morphological analysis of the adhesive interface using scanning electron microscopy (SEM) (n=5).
34910075	9	86	theme	interface	1325:1333	arg1	µTBS					1304:1307	the µTBS	1300:1307	the µTBS of the adhesive interface	1300:1333	RESULTS Chitosan improved the µTBS of the adhesive interface when compared with the control group (p=0.004).
34649174	8	0	theme	fragmentation	1181:1193	arg1	patterns					1195:1202	fragmentation patterns	1181:1202	fragmentation patterns observed in MS/MS data	1181:1225	Improvement in technology to remove sodium before MS results in minimal peak dispersion, allowing GOS degrees of polymerization 2 to 6 to be identified based on mass spectra obtained from intact oligosaccharides and confirmed using fragmentation patterns observed in MS/MS data.
34649174	8	1	theme	polymerization	1062:1075	arg1	degrees					1051:1057	GOS degrees	1047:1057	GOS degrees of polymerization 2 to 6	1047:1082	Improvement in technology to remove sodium before MS results in minimal peak dispersion, allowing GOS degrees of polymerization 2 to 6 to be identified based on mass spectra obtained from intact oligosaccharides and confirmed using fragmentation patterns observed in MS/MS data.
34649174	1	2	from	effects	86:92	arg1	health					155:160	digestive and immune health	134:160	digestive and immune health	134:160	Due to beneficial effects of galactosyl oligosaccharides (GOS) on digestive and immune health, their characterization has become increasingly important.
34649174	3	3	theme	High	342:345	arg1	chromatography					374:387	High performance anion exchange chromatography	342:387	High performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD)	342:433	High performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) is widely used for GOS characterization.
34649174	8	4	theme	minimal	1013:1019	arg1	dispersion					1026:1035	minimal peak dispersion	1013:1035	minimal peak dispersion	1013:1035	Improvement in technology to remove sodium before MS results in minimal peak dispersion, allowing GOS degrees of polymerization 2 to 6 to be identified based on mass spectra obtained from intact oligosaccharides and confirmed using fragmentation patterns observed in MS/MS data.
34649174	9	5	theme	oligosaccharides	1279:1294	arg1	identification					1258:1271	identification	1258:1271	identification of 28 oligosaccharides in a commercial GOS sample	1258:1321	Combining HPAE with MS led to identification of 28 oligosaccharides in a commercial GOS sample.
34649174	8	6	dep	technology	964:973	arg1	remove					978:983	remove	978:983	to remove sodium before MS	975:1000	Improvement in technology to remove sodium before MS results in minimal peak dispersion, allowing GOS degrees of polymerization 2 to 6 to be identified based on mass spectra obtained from intact oligosaccharides and confirmed using fragmentation patterns observed in MS/MS data.
34649174	6	7	dep	PAD	722:724	arg1	detections					762:771	detections	762:771	detections	762:771	Our approach combines high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections to provide in-depth information on GOS composition.
34649174	11	8	theme	HPAE	1506:1509	arg1	database					1528:1535	a comprehensive structure vs HPAE elution behavior database	1477:1535	database	1528:1535	To our knowledge this is first such attempt and can form a basis for a comprehensive structure vs HPAE elution behavior database.
34649174	6	9	theme	HPAE	650:653	arg1	separation					655:664	high resolution HPAE separation	634:664	high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections	634:771	Our approach combines high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections to provide in-depth information on GOS composition.
34649174	10	10	theme	oligosaccharide	1350:1364	arg1	structure					1366:1374	oligosaccharide structure	1350:1374	oligosaccharide structure	1350:1374	We attempted to correlate oligosaccharide structure with observed elution behavior.
34649174	3	11	theme	pulsed	394:399	arg1	HPAE-PAD					425:432	HPAE-PAD	425:432	HPAE-PAD	425:432	High performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) is widely used for GOS characterization.
34649174	3	11	theme	pulsed	394:399	arg1	detection					414:422	pulsed amperometric detection	394:422	pulsed amperometric detection (HPAE-PAD)	394:433	High performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) is widely used for GOS characterization.
34649174	6	12	with	column	686:691	arg1	size					712:715	4 µm particle size	698:715	4 µm particle size	698:715	Our approach combines high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections to provide in-depth information on GOS composition.
34649174	6	12	with	column	686:691	arg1	MS					758:759	MS	758:759	MS	758:759	Our approach combines high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections to provide in-depth information on GOS composition.
34649174	6	12	with	column	686:691	arg1	spectrometry					744:755	Orbitrap mass spectrometry	730:755	Orbitrap mass spectrometry (MS)	730:760	Our approach combines high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections to provide in-depth information on GOS composition.
34649174	6	12	with	column	686:691	arg1	PAD					722:724	PAD	722:724	PAD	722:724	Our approach combines high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections to provide in-depth information on GOS composition.
34649174	11	13	theme	first	1433:1437	arg1	attempt					1444:1450	first such attempt	1433:1450	first such attempt	1433:1450	To our knowledge this is first such attempt and can form a basis for a comprehensive structure vs HPAE elution behavior database.
34649174	6	14	theme	mass	739:742	arg1	MS					758:759	MS	758:759	MS	758:759	Our approach combines high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections to provide in-depth information on GOS composition.
34649174	6	14	theme	mass	739:742	arg1	spectrometry					744:755	Orbitrap mass spectrometry	730:755	Orbitrap mass spectrometry (MS)	730:760	Our approach combines high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections to provide in-depth information on GOS composition.
34649174	4	15	with	separate	514:521	arg1	power					500:504	its high resolving power	481:504	its high resolving power	481:504	With its high resolving power, it can separate structural isomers.
34649174	3	16	theme	exchange	365:372	arg1	chromatography					374:387	High performance anion exchange chromatography	342:387	High performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD)	342:433	High performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) is widely used for GOS characterization.
34649174	8	17	theme	peak	1021:1024	arg1	dispersion					1026:1035	minimal peak dispersion	1013:1035	minimal peak dispersion	1013:1035	Improvement in technology to remove sodium before MS results in minimal peak dispersion, allowing GOS degrees of polymerization 2 to 6 to be identified based on mass spectra obtained from intact oligosaccharides and confirmed using fragmentation patterns observed in MS/MS data.
34649174	1	18	theme	beneficial	75:84	arg1	effects					86:92	beneficial effects	75:92	beneficial effects of galactosyl oligosaccharides (GOS) on digestive and immune health	75:160	Due to beneficial effects of galactosyl oligosaccharides (GOS) on digestive and immune health, their characterization has become increasingly important.
34649174	6	19	theme	Orbitrap	730:737	arg1	MS					758:759	MS	758:759	MS	758:759	Our approach combines high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections to provide in-depth information on GOS composition.
34649174	6	19	theme	Orbitrap	730:737	arg1	spectrometry					744:755	Orbitrap mass spectrometry	730:755	Orbitrap mass spectrometry (MS)	730:760	Our approach combines high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections to provide in-depth information on GOS composition.
34649174	11	20	theme	comprehensive	1479:1491	arg1	structure					1493:1501	a comprehensive structure vs HPAE elution behavior database	1477:1535	structure	1493:1501	To our knowledge this is first such attempt and can form a basis for a comprehensive structure vs HPAE elution behavior database.
34649174	5	21	theme	used	598:601	arg1	methods					603:609	currently used methods	588:609	currently used methods	588:609	Here we present a significant improvement to currently used methods.
34649174	8	22	theme	MS/MS	1216:1220	arg1	data					1222:1225	MS/MS data	1216:1225	MS/MS data	1216:1225	Improvement in technology to remove sodium before MS results in minimal peak dispersion, allowing GOS degrees of polymerization 2 to 6 to be identified based on mass spectra obtained from intact oligosaccharides and confirmed using fragmentation patterns observed in MS/MS data.
34649174	0	23	theme	improved	3:10	arg1	method					12:17	An improved method	0:17	An improved method for galactosyl oligosaccharide characterization	0:65	An improved method for galactosyl oligosaccharide characterization.
34649174	7	24	from	resolution	841:850	arg1	region					884:889	the disaccharide region	867:889	the disaccharide region	867:889	Oligosaccharide resolution, especially in the disaccharide region, is significantly improved and can be routinely achieved.
34649174	6	25	theme	GOS	808:810	arg1	composition					812:822	GOS composition	808:822	GOS composition	808:822	Our approach combines high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections to provide in-depth information on GOS composition.
34649174	2	26	theme	linkages	332:339	arg1	oligosaccharides					292:307	oligosaccharides	292:307	oligosaccharides of different sizes and linkages	292:339	This is especially so as GOS are synthesized enzymatically and contain oligosaccharides of different sizes and linkages.
34649174	11	27	theme	behavior	1519:1526	arg1	database					1528:1535	a comprehensive structure vs HPAE elution behavior database	1477:1535	database	1528:1535	To our knowledge this is first such attempt and can form a basis for a comprehensive structure vs HPAE elution behavior database.
34649174	6	28	theme	high	634:637	arg1	separation					655:664	high resolution HPAE separation	634:664	high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections	634:771	Our approach combines high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections to provide in-depth information on GOS composition.
34649174	11	29	theme	such	1439:1442	arg1	attempt					1444:1450	first such attempt	1433:1450	first such attempt	1433:1450	To our knowledge this is first such attempt and can form a basis for a comprehensive structure vs HPAE elution behavior database.
34649174	1	30	theme	galactosyl	97:106	arg1	GOS					126:128	GOS	126:128	GOS	126:128	Due to beneficial effects of galactosyl oligosaccharides (GOS) on digestive and immune health, their characterization has become increasingly important.
34649174	1	30	theme	galactosyl	97:106	arg1	oligosaccharides					108:123	galactosyl oligosaccharides	97:123	galactosyl oligosaccharides (GOS)	97:129	Due to beneficial effects of galactosyl oligosaccharides (GOS) on digestive and immune health, their characterization has become increasingly important.
34649174	8	31	dep	polymerization	1062:1075	arg1	to					1079:1080	to	1079:1080	to	1079:1080	Improvement in technology to remove sodium before MS results in minimal peak dispersion, allowing GOS degrees of polymerization 2 to 6 to be identified based on mass spectra obtained from intact oligosaccharides and confirmed using fragmentation patterns observed in MS/MS data.
34649174	3	32	theme	GOS	454:456	arg1	characterization					458:473	GOS characterization	454:473	GOS characterization	454:473	High performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) is widely used for GOS characterization.
34649174	3	33	theme	amperometric	401:412	arg1	HPAE-PAD					425:432	HPAE-PAD	425:432	HPAE-PAD	425:432	High performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) is widely used for GOS characterization.
34649174	3	33	theme	amperometric	401:412	arg1	detection					414:422	pulsed amperometric detection	394:422	pulsed amperometric detection (HPAE-PAD)	394:433	High performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) is widely used for GOS characterization.
34649174	2	34	contain	contain	284:290	arg1	GOS					246:248	GOS	246:248	GOS	246:248	This is especially so as GOS are synthesized enzymatically and contain oligosaccharides of different sizes and linkages.
34649174	2	34	contain	contain	284:290	arg2	oligosaccharides					292:307	oligosaccharides	292:307	oligosaccharides of different sizes and linkages	292:339	This is especially so as GOS are synthesized enzymatically and contain oligosaccharides of different sizes and linkages.
34649174	8	35	theme	GOS	1047:1049	arg1	degrees					1051:1057	GOS degrees	1047:1057	GOS degrees of polymerization 2 to 6	1047:1082	Improvement in technology to remove sodium before MS results in minimal peak dispersion, allowing GOS degrees of polymerization 2 to 6 to be identified based on mass spectra obtained from intact oligosaccharides and confirmed using fragmentation patterns observed in MS/MS data.
34649174	2	36	theme	sizes	322:326	arg1	oligosaccharides					292:307	oligosaccharides	292:307	oligosaccharides of different sizes and linkages	292:339	This is especially so as GOS are synthesized enzymatically and contain oligosaccharides of different sizes and linkages.
34649174	7	37	theme	disaccharide	871:882	arg1	region					884:889	the disaccharide region	867:889	the disaccharide region	867:889	Oligosaccharide resolution, especially in the disaccharide region, is significantly improved and can be routinely achieved.
34649174	8	38	located	observed	1204:1211	arg1	data					1222:1225	MS/MS data	1216:1225	MS/MS data	1216:1225	Improvement in technology to remove sodium before MS results in minimal peak dispersion, allowing GOS degrees of polymerization 2 to 6 to be identified based on mass spectra obtained from intact oligosaccharides and confirmed using fragmentation patterns observed in MS/MS data.
34649174	8	38	located	observed	1204:1211	arg2	patterns					1195:1202	fragmentation patterns	1181:1202	fragmentation patterns observed in MS/MS data	1181:1225	Improvement in technology to remove sodium before MS results in minimal peak dispersion, allowing GOS degrees of polymerization 2 to 6 to be identified based on mass spectra obtained from intact oligosaccharides and confirmed using fragmentation patterns observed in MS/MS data.
34649174	2	39	theme	different	312:320	arg1	sizes					322:326	different sizes	312:326	different sizes	312:326	This is especially so as GOS are synthesized enzymatically and contain oligosaccharides of different sizes and linkages.
34649174	6	40	theme	particle	703:710	arg1	size					712:715	4 µm particle size	698:715	4 µm particle size	698:715	Our approach combines high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections to provide in-depth information on GOS composition.
34649174	6	41	from	information	793:803	arg1	composition					812:822	GOS composition	808:822	GOS composition	808:822	Our approach combines high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections to provide in-depth information on GOS composition.
34649174	0	42	theme	oligosaccharide	34:48	arg1	characterization					50:65	galactosyl oligosaccharide characterization	23:65	galactosyl oligosaccharide characterization	23:65	An improved method for galactosyl oligosaccharide characterization.
34649174	7	43	theme	Oligosaccharide	825:839	arg1	resolution					841:850	Oligosaccharide resolution	825:850	Oligosaccharide resolution	825:850	Oligosaccharide resolution, especially in the disaccharide region, is significantly improved and can be routinely achieved.
34649174	6	44	theme	4 µm	698:701	arg1	size					712:715	4 µm particle size	698:715	4 µm particle size	698:715	Our approach combines high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections to provide in-depth information on GOS composition.
34649174	0	45	theme	galactosyl	23:32	arg1	characterization					50:65	galactosyl oligosaccharide characterization	23:65	galactosyl oligosaccharide characterization	23:65	An improved method for galactosyl oligosaccharide characterization.
34649174	8	46	theme	mass	1110:1113	arg1	spectra					1115:1121	mass spectra	1110:1121	mass spectra obtained from intact oligosaccharides	1110:1159	Improvement in technology to remove sodium before MS results in minimal peak dispersion, allowing GOS degrees of polymerization 2 to 6 to be identified based on mass spectra obtained from intact oligosaccharides and confirmed using fragmentation patterns observed in MS/MS data.
34649174	4	47	theme	resolving	490:498	arg1	power					500:504	its high resolving power	481:504	its high resolving power	481:504	With its high resolving power, it can separate structural isomers.
34649174	3	48	with	chromatography	374:387	arg1	HPAE-PAD					425:432	HPAE-PAD	425:432	HPAE-PAD	425:432	High performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) is widely used for GOS characterization.
34649174	3	48	with	chromatography	374:387	arg1	detection					414:422	pulsed amperometric detection	394:422	pulsed amperometric detection (HPAE-PAD)	394:433	High performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) is widely used for GOS characterization.
34649174	5	49	theme	significant	561:571	arg1	improvement					573:583	a significant improvement	559:583	a significant improvement to currently used methods	559:609	Here we present a significant improvement to currently used methods.
34649174	9	50	from	sample	1316:1321	arg1	identification					1258:1271	identification	1258:1271	identification of 28 oligosaccharides in a commercial GOS sample	1258:1321	Combining HPAE with MS led to identification of 28 oligosaccharides in a commercial GOS sample.
34649174	4	51	theme	high	485:488	arg1	power					500:504	its high resolving power	481:504	its high resolving power	481:504	With its high resolving power, it can separate structural isomers.
34649174	10	52	theme	elution	1390:1396	arg1	behavior					1398:1405	observed elution behavior	1381:1405	observed elution behavior	1381:1405	We attempted to correlate oligosaccharide structure with observed elution behavior.
34649174	6	53	theme	resolution	639:648	arg1	separation					655:664	high resolution HPAE separation	634:664	high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections	634:771	Our approach combines high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections to provide in-depth information on GOS composition.
34649174	10	54	theme	observed	1381:1388	arg1	behavior					1398:1405	observed elution behavior	1381:1405	observed elution behavior	1381:1405	We attempted to correlate oligosaccharide structure with observed elution behavior.
34649174	3	55	used	used	445:448	arg2	chromatography					374:387	High performance anion exchange chromatography	342:387	High performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD)	342:433	High performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) is widely used for GOS characterization.
34649174	8	56	from	Improvement	949:959	arg1	technology					964:973	technology	964:973	technology to remove sodium before MS	964:1000	Improvement in technology to remove sodium before MS results in minimal peak dispersion, allowing GOS degrees of polymerization 2 to 6 to be identified based on mass spectra obtained from intact oligosaccharides and confirmed using fragmentation patterns observed in MS/MS data.
34649174	6	57	from	separation	655:664	arg1	column					686:691	a CarboPac PA300 column	669:691	a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections	669:771	Our approach combines high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections to provide in-depth information on GOS composition.
34649174	1	58	theme	oligosaccharides	108:123	arg1	effects					86:92	beneficial effects	75:92	beneficial effects of galactosyl oligosaccharides (GOS) on digestive and immune health	75:160	Due to beneficial effects of galactosyl oligosaccharides (GOS) on digestive and immune health, their characterization has become increasingly important.
34649174	8	59	theme	intact	1137:1142	arg1	oligosaccharides					1144:1159	intact oligosaccharides	1137:1159	intact oligosaccharides	1137:1159	Improvement in technology to remove sodium before MS results in minimal peak dispersion, allowing GOS degrees of polymerization 2 to 6 to be identified based on mass spectra obtained from intact oligosaccharides and confirmed using fragmentation patterns observed in MS/MS data.
34649174	9	60	theme	commercial	1301:1310	arg1	sample					1316:1321	a commercial GOS sample	1299:1321	a commercial GOS sample	1299:1321	Combining HPAE with MS led to identification of 28 oligosaccharides in a commercial GOS sample.
34649174	6	61	theme	in-depth	784:791	arg1	information					793:803	in-depth information	784:803	in-depth information on GOS composition	784:822	Our approach combines high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections to provide in-depth information on GOS composition.
34649174	1	62	theme	digestive	134:142	arg1	health					155:160	digestive and immune health	134:160	digestive and immune health	134:160	Due to beneficial effects of galactosyl oligosaccharides (GOS) on digestive and immune health, their characterization has become increasingly important.
34649174	6	63	theme	PA300	680:684	arg1	column					686:691	a CarboPac PA300 column	669:691	a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections	669:771	Our approach combines high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections to provide in-depth information on GOS composition.
34649174	11	64	theme	elution	1511:1517	arg1	database					1528:1535	a comprehensive structure vs HPAE elution behavior database	1477:1535	database	1528:1535	To our knowledge this is first such attempt and can form a basis for a comprehensive structure vs HPAE elution behavior database.
34649174	3	65	theme	performance	347:357	arg1	chromatography					374:387	High performance anion exchange chromatography	342:387	High performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD)	342:433	High performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) is widely used for GOS characterization.
34649174	9	66	theme	GOS	1312:1314	arg1	sample					1316:1321	a commercial GOS sample	1299:1321	a commercial GOS sample	1299:1321	Combining HPAE with MS led to identification of 28 oligosaccharides in a commercial GOS sample.
34649174	6	67	theme	CarboPac	671:678	arg1	column					686:691	a CarboPac PA300 column	669:691	a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections	669:771	Our approach combines high resolution HPAE separation on a CarboPac PA300 column with 4 µm particle size with PAD and Orbitrap mass spectrometry (MS) detections to provide in-depth information on GOS composition.
34649174	3	68	theme	anion	359:363	arg1	chromatography					374:387	High performance anion exchange chromatography	342:387	High performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD)	342:433	High performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) is widely used for GOS characterization.
34649174	4	69	theme	structural	523:532	arg1	isomers					534:540	structural isomers	523:540	structural isomers	523:540	With its high resolving power, it can separate structural isomers.
34649174	1	70	theme	immune	148:153	arg1	health					155:160	digestive and immune health	134:160	digestive and immune health	134:160	Due to beneficial effects of galactosyl oligosaccharides (GOS) on digestive and immune health, their characterization has become increasingly important.
34649174	9	71	from	identification	1258:1271	arg1	sample					1316:1321	a commercial GOS sample	1299:1321	a commercial GOS sample	1299:1321	Combining HPAE with MS led to identification of 28 oligosaccharides in a commercial GOS sample.
34649174	9	72	from	oligosaccharides	1279:1294	arg1	sample					1316:1321	a commercial GOS sample	1299:1321	a commercial GOS sample	1299:1321	Combining HPAE with MS led to identification of 28 oligosaccharides in a commercial GOS sample.
32228905	0	0	theme	wound-dressing	86:99	arg1	material					101:108	a potential wound-dressing material	74:108	a potential wound-dressing material	74:108	Polymer-based wafers containing in situ synthesized gold nanoparticles as a potential wound-dressing material.
32228905	0	0	theme	wound-dressing	86:99	arg1	nanoparticles					57:69	in situ synthesized gold nanoparticles	32:69	in situ synthesized gold nanoparticles	32:69	Polymer-based wafers containing in situ synthesized gold nanoparticles as a potential wound-dressing material.
32228905	5	1	theme	bell-shaped	708:718	arg1	band					738:741	a single bell-shaped UV-vis absorption band	699:741	a single bell-shaped UV-vis absorption band centered ~ 540 nm	699:759	The AuNPs obtained were spherical in shape (~ 10-15 nm in diameter) and exhibited a single bell-shaped UV-vis absorption band centered ~ 540 nm.
32228905	9	2	theme	promising	1492:1500	arg1	wafers					1457:1462	the produced wafers	1444:1462	the produced wafers containing AuNPs	1444:1479	These findings endorsed the claim that the produced wafers containing AuNPs could be a promising material for wound dressing applications.
32228905	9	2	theme	promising	1492:1500	arg1	material					1502:1509	a promising material	1490:1509	a promising material for wound dressing applications	1490:1541	These findings endorsed the claim that the produced wafers containing AuNPs could be a promising material for wound dressing applications.
32228905	3	3	theme	morphological	464:476	arg1	properties					504:513	their physicochemical, morphological, mechanical, and swelling properties	441:513	their physicochemical, morphological, mechanical, and swelling properties	441:513	The produced wafers containing AuNPs were investigated for their physicochemical, morphological, mechanical, and swelling properties.
32228905	5	4	from	10-15 nm	663:670	arg1	diameter					675:682	diameter	675:682	diameter	675:682	The AuNPs obtained were spherical in shape (~ 10-15 nm in diameter) and exhibited a single bell-shaped UV-vis absorption band centered ~ 540 nm.
32228905	0	5	theme	potential	76:84	arg1	material					101:108	a potential wound-dressing material	74:108	a potential wound-dressing material	74:108	Polymer-based wafers containing in situ synthesized gold nanoparticles as a potential wound-dressing material.
32228905	0	5	theme	potential	76:84	arg1	nanoparticles					57:69	in situ synthesized gold nanoparticles	32:69	in situ synthesized gold nanoparticles	32:69	Polymer-based wafers containing in situ synthesized gold nanoparticles as a potential wound-dressing material.
32228905	1	6	theme	%	298:298	arg1	ratios					262:267	ratios	262:267	ratios of 42/22/13% w/w and 35/15/17% w/w	262:302	Polymer-based wafers containing gold nanoparticles (AuNP) were prepared using κ-carrageenan (κC), locust bean gum (LBG) and polyvinyl alcohol (PVA) at ratios of 42/22/13% w/w and 35/15/17% w/w.
32228905	6	7	contain	containing	790:799	arg1	wafers					783:788	the wafers	779:788	the wafers containing AuNPs	779:805	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	6	7	contain	containing	790:799	arg2	AuNPs					801:805	AuNPs	801:805	AuNPs	801:805	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	1	8	theme	polyvinyl	235:243	arg1	PVA					254:256	PVA	254:256	PVA	254:256	Polymer-based wafers containing gold nanoparticles (AuNP) were prepared using κ-carrageenan (κC), locust bean gum (LBG) and polyvinyl alcohol (PVA) at ratios of 42/22/13% w/w and 35/15/17% w/w.
32228905	1	8	theme	polyvinyl	235:243	arg1	alcohol					245:251	polyvinyl alcohol	235:251	polyvinyl alcohol (PVA)	235:257	Polymer-based wafers containing gold nanoparticles (AuNP) were prepared using κ-carrageenan (κC), locust bean gum (LBG) and polyvinyl alcohol (PVA) at ratios of 42/22/13% w/w and 35/15/17% w/w.
32228905	1	9	theme	w/w	300:302	arg1	%					298:298	35/15/17% w/w	290:302	35/15/17% w/w	290:302	Polymer-based wafers containing gold nanoparticles (AuNP) were prepared using κ-carrageenan (κC), locust bean gum (LBG) and polyvinyl alcohol (PVA) at ratios of 42/22/13% w/w and 35/15/17% w/w.
32228905	6	10	theme	FT-IR	762:766	arg1	spectra					768:774	FT-IR spectra	762:774	FT-IR spectra of the wafers containing AuNPs	762:805	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	6	11	theme	due	943:945	arg1	wavenumber					932:941	a higher wavenumber	923:941	a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively	923:1047	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	4	12	theme	in	560:561	arg1	cytotoxicity					569:580	in vitro cytotoxicity	560:580	in vitro cytotoxicity	560:580	In addition, bacterial barrier activity and in vitro cytotoxicity were also evaluated in this study.
32228905	7	13	theme	porous	1075:1080	arg1	structure					1082:1090	the porous structure	1071:1090	the porous structure of the produced wafers	1071:1113	SEM images confirmed the porous structure of the produced wafers, being the surface area, mechanical properties, and swelling behavior directly affected by changing both the initial amount of [Au+3] and the composition of the wafers.
32228905	6	14	theme	O=S=O	830:834	arg1	band					848:851	ν(O=S=O) absorption band	828:851	ν(O=S=O) absorption band	828:851	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	9	15	theme	wound	1515:1519	arg1	applications					1530:1541	wound dressing applications	1515:1541	wound dressing applications	1515:1541	These findings endorsed the claim that the produced wafers containing AuNPs could be a promising material for wound dressing applications.
32228905	6	16	theme	lower	862:866	arg1	wavenumber					868:877	a lower wavenumber	860:877	a lower wavenumber	860:877	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	5	17	dep	spherical	641:649	arg1	AuNPs					621:625	The AuNPs	617:625	The AuNPs obtained	617:634	The AuNPs obtained were spherical in shape (~ 10-15 nm in diameter) and exhibited a single bell-shaped UV-vis absorption band centered ~ 540 nm.
32228905	5	17	dep	spherical	641:649	arg1	10-15 nm					663:670	~ 10-15 nm	661:670	~ 10-15 nm in diameter	661:682	The AuNPs obtained were spherical in shape (~ 10-15 nm in diameter) and exhibited a single bell-shaped UV-vis absorption band centered ~ 540 nm.
32228905	5	17	dep	spherical	641:649	arg1	spherical					641:649	spherical	641:649	spherical	641:649	The AuNPs obtained were spherical in shape (~ 10-15 nm in diameter) and exhibited a single bell-shaped UV-vis absorption band centered ~ 540 nm.
32228905	5	18	dep	centered	743:750	arg1	540 nm					754:759	~ 540 nm	752:759	~ 540 nm	752:759	The AuNPs obtained were spherical in shape (~ 10-15 nm in diameter) and exhibited a single bell-shaped UV-vis absorption band centered ~ 540 nm.
32228905	8	19	theme	NIH-3T3	1335:1341	arg1	cells					1354:1358	NIH-3T3 fibroblast cells	1335:1358	NIH-3T3 fibroblast cells	1335:1358	Lastly, the produced wafers showed non-toxicity to NIH-3T3 fibroblast cells, and they also serve as a bacterial barrier.
32228905	6	20	theme	higher	925:930	arg1	wavenumber					932:941	a higher wavenumber	923:941	a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively	923:1047	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	3	21	theme	produced	386:393	arg1	wafers					395:400	The produced wafers	382:400	The produced wafers containing AuNPs	382:417	The produced wafers containing AuNPs were investigated for their physicochemical, morphological, mechanical, and swelling properties.
32228905	0	22	theme	gold	52:55	arg1	material					101:108	a potential wound-dressing material	74:108	a potential wound-dressing material	74:108	Polymer-based wafers containing in situ synthesized gold nanoparticles as a potential wound-dressing material.
32228905	0	22	theme	gold	52:55	arg1	nanoparticles					57:69	in situ synthesized gold nanoparticles	32:69	in situ synthesized gold nanoparticles	32:69	Polymer-based wafers containing in situ synthesized gold nanoparticles as a potential wound-dressing material.
32228905	6	23	theme	ν	828:828	arg1	band					848:851	ν(O=S=O) absorption band	828:851	ν(O=S=O) absorption band	828:851	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	5	24	theme	absorption	727:736	arg1	band					738:741	a single bell-shaped UV-vis absorption band	699:741	a single bell-shaped UV-vis absorption band centered ~ 540 nm	699:759	The AuNPs obtained were spherical in shape (~ 10-15 nm in diameter) and exhibited a single bell-shaped UV-vis absorption band centered ~ 540 nm.
32228905	3	25	theme	swelling	495:502	arg1	properties					504:513	their physicochemical, morphological, mechanical, and swelling properties	441:513	their physicochemical, morphological, mechanical, and swelling properties	441:513	The produced wafers containing AuNPs were investigated for their physicochemical, morphological, mechanical, and swelling properties.
32228905	6	26	theme	OH	970:971	arg1	groups					973:978	OH groups	970:978	OH groups	970:978	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	9	27	contain	containing	1464:1473	arg1	wafers					1457:1462	the produced wafers	1444:1462	the produced wafers containing AuNPs	1444:1479	These findings endorsed the claim that the produced wafers containing AuNPs could be a promising material for wound dressing applications.
32228905	9	27	contain	containing	1464:1473	arg2	AuNPs					1475:1479	AuNPs	1475:1479	AuNPs	1475:1479	These findings endorsed the claim that the produced wafers containing AuNPs could be a promising material for wound dressing applications.
32228905	9	27	contain	containing	1464:1473	arg1	material					1502:1509	a promising material	1490:1509	a promising material for wound dressing applications	1490:1541	These findings endorsed the claim that the produced wafers containing AuNPs could be a promising material for wound dressing applications.
32228905	5	28	theme	UV-vis	720:725	arg1	band					738:741	a single bell-shaped UV-vis absorption band	699:741	a single bell-shaped UV-vis absorption band centered ~ 540 nm	699:759	The AuNPs obtained were spherical in shape (~ 10-15 nm in diameter) and exhibited a single bell-shaped UV-vis absorption band centered ~ 540 nm.
32228905	7	29	theme	produced	1099:1106	arg1	wafers					1108:1113	the produced wafers	1095:1113	the produced wafers	1095:1113	SEM images confirmed the porous structure of the produced wafers, being the surface area, mechanical properties, and swelling behavior directly affected by changing both the initial amount of [Au+3] and the composition of the wafers.
32228905	0	30	theme	Polymer-based	0:12	arg1	wafers					14:19	Polymer-based wafers	0:19	Polymer-based wafers	0:19	Polymer-based wafers containing in situ synthesized gold nanoparticles as a potential wound-dressing material.
32228905	6	31	theme	ν	894:894	arg1	band					911:914	ν(OH) absorption band	894:914	ν(OH) absorption band	894:914	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	8	32	theme	fibroblast	1343:1352	arg1	cells					1354:1358	NIH-3T3 fibroblast cells	1335:1358	NIH-3T3 fibroblast cells	1335:1358	Lastly, the produced wafers showed non-toxicity to NIH-3T3 fibroblast cells, and they also serve as a bacterial barrier.
32228905	7	33	theme	wafers	1276:1281	arg1	amount					1232:1237	the initial amount	1220:1237	the initial amount of [Au+3]	1220:1247	SEM images confirmed the porous structure of the produced wafers, being the surface area, mechanical properties, and swelling behavior directly affected by changing both the initial amount of [Au+3] and the composition of the wafers.
32228905	7	33	theme	wafers	1276:1281	arg1	composition					1257:1267	the composition	1253:1267	the composition of the wafers	1253:1281	SEM images confirmed the porous structure of the produced wafers, being the surface area, mechanical properties, and swelling behavior directly affected by changing both the initial amount of [Au+3] and the composition of the wafers.
32228905	7	33	theme	wafers	1276:1281	arg1	wafers					1276:1281	the wafers	1272:1281	the wafers	1272:1281	SEM images confirmed the porous structure of the produced wafers, being the surface area, mechanical properties, and swelling behavior directly affected by changing both the initial amount of [Au+3] and the composition of the wafers.
32228905	7	33	theme	wafers	1276:1281	arg1	[Au+3					1242:1246	[Au+3]	1242:1247	[Au+3]	1242:1247	SEM images confirmed the porous structure of the produced wafers, being the surface area, mechanical properties, and swelling behavior directly affected by changing both the initial amount of [Au+3] and the composition of the wafers.
32228905	6	34	theme	absorption	900:909	arg1	band					911:914	ν(OH) absorption band	894:914	ν(OH) absorption band	894:914	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	6	35	with	coordination	954:965	arg1	κC					1032:1033	κC	1032:1033	κC	1032:1033	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	6	35	with	coordination	954:965	arg1	groups					1022:1027	O=S=O groups	1016:1027	O=S=O groups	1016:1027	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	0	36	theme	in	32:33	arg1	material					101:108	a potential wound-dressing material	74:108	a potential wound-dressing material	74:108	Polymer-based wafers containing in situ synthesized gold nanoparticles as a potential wound-dressing material.
32228905	0	36	theme	in	32:33	arg1	nanoparticles					57:69	in situ synthesized gold nanoparticles	32:69	in situ synthesized gold nanoparticles	32:69	Polymer-based wafers containing in situ synthesized gold nanoparticles as a potential wound-dressing material.
32228905	6	37	with	interaction	999:1009	arg1	κC					1032:1033	κC	1032:1033	κC	1032:1033	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	6	37	with	interaction	999:1009	arg1	groups					1022:1027	O=S=O groups	1016:1027	O=S=O groups	1016:1027	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	7	38	theme	initial	1224:1230	arg1	amount					1232:1237	the initial amount	1220:1237	the initial amount of [Au+3]	1220:1247	SEM images confirmed the porous structure of the produced wafers, being the surface area, mechanical properties, and swelling behavior directly affected by changing both the initial amount of [Au+3] and the composition of the wafers.
32228905	7	38	theme	initial	1224:1230	arg1	wafers					1276:1281	the wafers	1272:1281	the wafers	1272:1281	SEM images confirmed the porous structure of the produced wafers, being the surface area, mechanical properties, and swelling behavior directly affected by changing both the initial amount of [Au+3] and the composition of the wafers.
32228905	7	38	theme	initial	1224:1230	arg1	[Au+3					1242:1246	[Au+3]	1242:1247	[Au+3]	1242:1247	SEM images confirmed the porous structure of the produced wafers, being the surface area, mechanical properties, and swelling behavior directly affected by changing both the initial amount of [Au+3] and the composition of the wafers.
32228905	1	39	theme	Polymer-based	111:123	arg1	wafers					125:130	Polymer-based wafers	111:130	Polymer-based wafers containing gold nanoparticles (AuNP)	111:167	Polymer-based wafers containing gold nanoparticles (AuNP) were prepared using κ-carrageenan (κC), locust bean gum (LBG) and polyvinyl alcohol (PVA) at ratios of 42/22/13% w/w and 35/15/17% w/w.
32228905	7	40	theme	swelling	1167:1174	arg1	behavior					1176:1183	swelling behavior	1167:1183	swelling behavior	1167:1183	SEM images confirmed the porous structure of the produced wafers, being the surface area, mechanical properties, and swelling behavior directly affected by changing both the initial amount of [Au+3] and the composition of the wafers.
32228905	4	41	theme	bacterial	529:537	arg1	activity					547:554	bacterial barrier activity	529:554	bacterial barrier activity	529:554	In addition, bacterial barrier activity and in vitro cytotoxicity were also evaluated in this study.
32228905	5	42	theme	~	661:661	arg1	AuNPs					621:625	The AuNPs	617:625	The AuNPs obtained	617:634	The AuNPs obtained were spherical in shape (~ 10-15 nm in diameter) and exhibited a single bell-shaped UV-vis absorption band centered ~ 540 nm.
32228905	5	42	theme	~	661:661	arg1	10-15 nm					663:670	~ 10-15 nm	661:670	~ 10-15 nm in diameter	661:682	The AuNPs obtained were spherical in shape (~ 10-15 nm in diameter) and exhibited a single bell-shaped UV-vis absorption band centered ~ 540 nm.
32228905	5	42	theme	~	661:661	arg1	spherical					641:649	spherical	641:649	spherical	641:649	The AuNPs obtained were spherical in shape (~ 10-15 nm in diameter) and exhibited a single bell-shaped UV-vis absorption band centered ~ 540 nm.
32228905	4	43	dep	in	560:561	arg1	vitro					563:567	vitro	563:567	vitro	563:567	In addition, bacterial barrier activity and in vitro cytotoxicity were also evaluated in this study.
32228905	6	44	theme	band	848:851	arg1	shift					885:889	a shift	883:889	a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively	883:1047	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	6	44	theme	band	848:851	arg1	shift					819:823	a shift	817:823	a shift of ν(O=S=O) absorption band toward a lower wavenumber	817:877	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	7	45	theme	surface	1126:1132	arg1	area					1134:1137	the surface area	1122:1137	the surface area	1122:1137	SEM images confirmed the porous structure of the produced wafers, being the surface area, mechanical properties, and swelling behavior directly affected by changing both the initial amount of [Au+3] and the composition of the wafers.
32228905	0	46	theme	synthesized	40:50	arg1	material					101:108	a potential wound-dressing material	74:108	a potential wound-dressing material	74:108	Polymer-based wafers containing in situ synthesized gold nanoparticles as a potential wound-dressing material.
32228905	0	46	theme	synthesized	40:50	arg1	nanoparticles					57:69	in situ synthesized gold nanoparticles	32:69	in situ synthesized gold nanoparticles	32:69	Polymer-based wafers containing in situ synthesized gold nanoparticles as a potential wound-dressing material.
32228905	6	47	theme	O=S=O	1016:1020	arg1	κC					1032:1033	κC	1032:1033	κC	1032:1033	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	6	47	theme	O=S=O	1016:1020	arg1	groups					1022:1027	O=S=O groups	1016:1027	O=S=O groups	1016:1027	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	3	48	contain	containing	402:411	arg2	AuNPs					413:417	AuNPs	413:417	AuNPs	413:417	The produced wafers containing AuNPs were investigated for their physicochemical, morphological, mechanical, and swelling properties.
32228905	3	48	contain	containing	402:411	arg1	wafers					395:400	The produced wafers	382:400	The produced wafers containing AuNPs	382:417	The produced wafers containing AuNPs were investigated for their physicochemical, morphological, mechanical, and swelling properties.
32228905	1	49	theme	locust	209:214	arg1	LBG					226:228	LBG	226:228	LBG	226:228	Polymer-based wafers containing gold nanoparticles (AuNP) were prepared using κ-carrageenan (κC), locust bean gum (LBG) and polyvinyl alcohol (PVA) at ratios of 42/22/13% w/w and 35/15/17% w/w.
32228905	1	49	theme	locust	209:214	arg1	gum					221:223	locust bean gum	209:223	locust bean gum (LBG)	209:229	Polymer-based wafers containing gold nanoparticles (AuNP) were prepared using κ-carrageenan (κC), locust bean gum (LBG) and polyvinyl alcohol (PVA) at ratios of 42/22/13% w/w and 35/15/17% w/w.
32228905	6	50	theme	groups	973:978	arg1	interaction					999:1009	their interaction	993:1009	their interaction with O=S=O groups of κC, respectively	993:1047	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	6	50	theme	groups	973:978	arg1	coordination					954:965	the coordination	950:965	the coordination of OH groups to AuNPs	950:987	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	4	51	theme	barrier	539:545	arg1	activity					547:554	bacterial barrier activity	529:554	bacterial barrier activity	529:554	In addition, bacterial barrier activity and in vitro cytotoxicity were also evaluated in this study.
32228905	9	52	theme	produced	1448:1455	arg1	wafers					1457:1462	the produced wafers	1444:1462	the produced wafers containing AuNPs	1444:1479	These findings endorsed the claim that the produced wafers containing AuNPs could be a promising material for wound dressing applications.
32228905	9	52	theme	produced	1448:1455	arg1	material					1502:1509	a promising material	1490:1509	a promising material for wound dressing applications	1490:1541	These findings endorsed the claim that the produced wafers containing AuNPs could be a promising material for wound dressing applications.
32228905	1	53	theme	bean	216:219	arg1	LBG					226:228	LBG	226:228	LBG	226:228	Polymer-based wafers containing gold nanoparticles (AuNP) were prepared using κ-carrageenan (κC), locust bean gum (LBG) and polyvinyl alcohol (PVA) at ratios of 42/22/13% w/w and 35/15/17% w/w.
32228905	1	53	theme	bean	216:219	arg1	gum					221:223	locust bean gum	209:223	locust bean gum (LBG)	209:229	Polymer-based wafers containing gold nanoparticles (AuNP) were prepared using κ-carrageenan (κC), locust bean gum (LBG) and polyvinyl alcohol (PVA) at ratios of 42/22/13% w/w and 35/15/17% w/w.
32228905	5	54	theme	centered	743:750	arg1	band					738:741	a single bell-shaped UV-vis absorption band	699:741	a single bell-shaped UV-vis absorption band centered ~ 540 nm	699:759	The AuNPs obtained were spherical in shape (~ 10-15 nm in diameter) and exhibited a single bell-shaped UV-vis absorption band centered ~ 540 nm.
32228905	7	55	theme	[Au+3	1242:1246	arg1	amount					1232:1237	the initial amount	1220:1237	the initial amount of [Au+3]	1220:1247	SEM images confirmed the porous structure of the produced wafers, being the surface area, mechanical properties, and swelling behavior directly affected by changing both the initial amount of [Au+3] and the composition of the wafers.
32228905	7	55	theme	[Au+3	1242:1246	arg1	composition					1257:1267	the composition	1253:1267	the composition of the wafers	1253:1281	SEM images confirmed the porous structure of the produced wafers, being the surface area, mechanical properties, and swelling behavior directly affected by changing both the initial amount of [Au+3] and the composition of the wafers.
32228905	7	55	theme	[Au+3	1242:1246	arg1	wafers					1276:1281	the wafers	1272:1281	the wafers	1272:1281	SEM images confirmed the porous structure of the produced wafers, being the surface area, mechanical properties, and swelling behavior directly affected by changing both the initial amount of [Au+3] and the composition of the wafers.
32228905	7	55	theme	[Au+3	1242:1246	arg1	[Au+3					1242:1246	[Au+3]	1242:1247	[Au+3]	1242:1247	SEM images confirmed the porous structure of the produced wafers, being the surface area, mechanical properties, and swelling behavior directly affected by changing both the initial amount of [Au+3] and the composition of the wafers.
32228905	8	56	theme	bacterial	1386:1394	arg1	they					1365:1368	they	1365:1368	they	1365:1368	Lastly, the produced wafers showed non-toxicity to NIH-3T3 fibroblast cells, and they also serve as a bacterial barrier.
32228905	8	56	theme	bacterial	1386:1394	arg1	barrier					1396:1402	a bacterial barrier	1384:1402	a bacterial barrier	1384:1402	Lastly, the produced wafers showed non-toxicity to NIH-3T3 fibroblast cells, and they also serve as a bacterial barrier.
32228905	3	57	theme	physicochemical	447:461	arg1	properties					504:513	their physicochemical, morphological, mechanical, and swelling properties	441:513	their physicochemical, morphological, mechanical, and swelling properties	441:513	The produced wafers containing AuNPs were investigated for their physicochemical, morphological, mechanical, and swelling properties.
32228905	1	58	contain	containing	132:141	arg2	nanoparticles					148:160	gold nanoparticles	143:160	gold nanoparticles (AuNP)	143:167	Polymer-based wafers containing gold nanoparticles (AuNP) were prepared using κ-carrageenan (κC), locust bean gum (LBG) and polyvinyl alcohol (PVA) at ratios of 42/22/13% w/w and 35/15/17% w/w.
32228905	1	58	contain	containing	132:141	arg2	AuNP					163:166	AuNP	163:166	AuNP	163:166	Polymer-based wafers containing gold nanoparticles (AuNP) were prepared using κ-carrageenan (κC), locust bean gum (LBG) and polyvinyl alcohol (PVA) at ratios of 42/22/13% w/w and 35/15/17% w/w.
32228905	1	58	contain	containing	132:141	arg1	wafers					125:130	Polymer-based wafers	111:130	Polymer-based wafers containing gold nanoparticles (AuNP)	111:167	Polymer-based wafers containing gold nanoparticles (AuNP) were prepared using κ-carrageenan (κC), locust bean gum (LBG) and polyvinyl alcohol (PVA) at ratios of 42/22/13% w/w and 35/15/17% w/w.
32228905	1	59	theme	%	280:280	arg1	ratios					262:267	ratios	262:267	ratios of 42/22/13% w/w and 35/15/17% w/w	262:302	Polymer-based wafers containing gold nanoparticles (AuNP) were prepared using κ-carrageenan (κC), locust bean gum (LBG) and polyvinyl alcohol (PVA) at ratios of 42/22/13% w/w and 35/15/17% w/w.
32228905	6	60	theme	absorption	837:846	arg1	band					848:851	ν(O=S=O) absorption band	828:851	ν(O=S=O) absorption band	828:851	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	7	61	theme	SEM	1050:1052	arg1	images					1054:1059	SEM images	1050:1059	SEM images	1050:1059	SEM images confirmed the porous structure of the produced wafers, being the surface area, mechanical properties, and swelling behavior directly affected by changing both the initial amount of [Au+3] and the composition of the wafers.
32228905	1	62	theme	gold	143:146	arg1	nanoparticles					148:160	gold nanoparticles	143:160	gold nanoparticles (AuNP)	143:167	Polymer-based wafers containing gold nanoparticles (AuNP) were prepared using κ-carrageenan (κC), locust bean gum (LBG) and polyvinyl alcohol (PVA) at ratios of 42/22/13% w/w and 35/15/17% w/w.
32228905	1	62	theme	gold	143:146	arg1	AuNP					163:166	AuNP	163:166	AuNP	163:166	Polymer-based wafers containing gold nanoparticles (AuNP) were prepared using κ-carrageenan (κC), locust bean gum (LBG) and polyvinyl alcohol (PVA) at ratios of 42/22/13% w/w and 35/15/17% w/w.
32228905	8	63	theme	produced	1296:1303	arg1	wafers					1305:1310	the produced wafers	1292:1310	the produced wafers	1292:1310	Lastly, the produced wafers showed non-toxicity to NIH-3T3 fibroblast cells, and they also serve as a bacterial barrier.
32228905	1	64	theme	w/w	282:284	arg1	%					280:280	42/22/13% w/w	272:284	42/22/13% w/w	272:284	Polymer-based wafers containing gold nanoparticles (AuNP) were prepared using κ-carrageenan (κC), locust bean gum (LBG) and polyvinyl alcohol (PVA) at ratios of 42/22/13% w/w and 35/15/17% w/w.
32228905	6	65	theme	wafers	783:788	arg1	spectra					768:774	FT-IR spectra	762:774	FT-IR spectra of the wafers containing AuNPs	762:805	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	0	66	dep	in	32:33	arg1	situ					35:38	situ	35:38	situ	35:38	Polymer-based wafers containing in situ synthesized gold nanoparticles as a potential wound-dressing material.
32228905	6	67	theme	κC	1032:1033	arg1	κC					1032:1033	κC	1032:1033	κC	1032:1033	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	6	67	theme	κC	1032:1033	arg1	groups					1022:1027	O=S=O groups	1016:1027	O=S=O groups	1016:1027	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	6	68	theme	OH	896:897	arg1	band					911:914	ν(OH) absorption band	894:914	ν(OH) absorption band	894:914	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	7	69	theme	mechanical	1140:1149	arg1	properties					1151:1160	mechanical properties	1140:1160	mechanical properties	1140:1160	SEM images confirmed the porous structure of the produced wafers, being the surface area, mechanical properties, and swelling behavior directly affected by changing both the initial amount of [Au+3] and the composition of the wafers.
32228905	5	70	from	shape	654:658	arg1	10-15 nm					663:670	~ 10-15 nm	661:670	~ 10-15 nm in diameter	661:682	The AuNPs obtained were spherical in shape (~ 10-15 nm in diameter) and exhibited a single bell-shaped UV-vis absorption band centered ~ 540 nm.
32228905	5	70	from	shape	654:658	arg1	AuNPs					621:625	The AuNPs	617:625	The AuNPs obtained	617:634	The AuNPs obtained were spherical in shape (~ 10-15 nm in diameter) and exhibited a single bell-shaped UV-vis absorption band centered ~ 540 nm.
32228905	5	70	from	shape	654:658	arg1	spherical					641:649	spherical	641:649	spherical	641:649	The AuNPs obtained were spherical in shape (~ 10-15 nm in diameter) and exhibited a single bell-shaped UV-vis absorption band centered ~ 540 nm.
32228905	7	71	theme	wafers	1108:1113	arg1	structure					1082:1090	the porous structure	1071:1090	the porous structure of the produced wafers	1071:1113	SEM images confirmed the porous structure of the produced wafers, being the surface area, mechanical properties, and swelling behavior directly affected by changing both the initial amount of [Au+3] and the composition of the wafers.
32228905	2	72	theme	particle	352:359	arg1	size					361:364	their particle size	346:364	their particle size	346:364	The synthesized AuNPs were evaluated for their particle size and morphology.
32228905	3	73	theme	mechanical	479:488	arg1	properties					504:513	their physicochemical, morphological, mechanical, and swelling properties	441:513	their physicochemical, morphological, mechanical, and swelling properties	441:513	The produced wafers containing AuNPs were investigated for their physicochemical, morphological, mechanical, and swelling properties.
32228905	5	74	theme	single	701:706	arg1	band					738:741	a single bell-shaped UV-vis absorption band	699:741	a single bell-shaped UV-vis absorption band centered ~ 540 nm	699:759	The AuNPs obtained were spherical in shape (~ 10-15 nm in diameter) and exhibited a single bell-shaped UV-vis absorption band centered ~ 540 nm.
32228905	9	75	theme	dressing	1521:1528	arg1	applications					1530:1541	wound dressing applications	1515:1541	wound dressing applications	1515:1541	These findings endorsed the claim that the produced wafers containing AuNPs could be a promising material for wound dressing applications.
32228905	6	76	theme	band	911:914	arg1	shift					885:889	a shift	883:889	a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively	883:1047	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	6	76	theme	band	911:914	arg1	shift					819:823	a shift	817:823	a shift of ν(O=S=O) absorption band toward a lower wavenumber	817:877	FT-IR spectra of the wafers containing AuNPs exhibited a shift of ν(O=S=O) absorption band toward a lower wavenumber and a shift of ν(OH) absorption band toward a higher wavenumber due to the coordination of OH groups to AuNPs and their interaction with O=S=O groups of κC, respectively.
32228905	2	77	theme	synthesized	309:319	arg1	AuNPs					321:325	The synthesized AuNPs	305:325	The synthesized AuNPs	305:325	The synthesized AuNPs were evaluated for their particle size and morphology.
32228905	5	78	from	spherical	641:649	arg1	shape					654:658	shape	654:658	shape	654:658	The AuNPs obtained were spherical in shape (~ 10-15 nm in diameter) and exhibited a single bell-shaped UV-vis absorption band centered ~ 540 nm.
32294500	9	0	theme	non-Fickian	1592:1602	arg1	behavior					1614:1621	non-Fickian transport behavior	1592:1621	non-Fickian transport behavior	1592:1621	The pharmacokinetic investigation of the releasing results demonstrated the best fitting with Korsmeyer-Peppas with diffusion exponent (n) values related to non-Fickian transport behavior suggesting a combination of erosion and diffusion mechanisms.
32294500	9	1	dep	fitting	1516:1522	arg1	n					1571:1571	n	1571:1571	n	1571:1571	The pharmacokinetic investigation of the releasing results demonstrated the best fitting with Korsmeyer-Peppas with diffusion exponent (n) values related to non-Fickian transport behavior suggesting a combination of erosion and diffusion mechanisms.
32294500	3	2	theme	420 mg/g	609:616	arg1	loading					626:632	420 mg/g maximum loading	609:632	420 mg/g maximum loading	609:632	The investigated composites are of 328 mg/g, 360 mg/g, 479 mg/g, and 420 mg/g maximum loading capacities for MCM-48, MCM-48/ST, MCM-48/CH, and MCM-48/β-CD, respectively.
32294500	4	3	theme	loading	808:814	arg1	mechanisms					816:825	physisorption loading mechanisms	794:825	physisorption loading mechanisms	794:825	The kinetic studies revealed preference for the Pseudo-first order model suggesting physisorption loading mechanisms.
32294500	5	4	theme	thermodynamic	850:862	arg1	parameters					864:873	the thermodynamic parameters	846:873	the thermodynamic parameters	846:873	This supported by the thermodynamic parameters and the recognized adsorption energies that are 0.55 KJ/mol (MCM-48), 0.63 KJ/mol (MCM-48/ST), 0.65 KJ/mol (MCM-48/CH), and 0.65 KJ/mol (MCM-48/β-CD).
32294500	9	5	theme	transport	1604:1612	arg1	behavior					1614:1621	non-Fickian transport behavior	1592:1621	non-Fickian transport behavior	1592:1621	The pharmacokinetic investigation of the releasing results demonstrated the best fitting with Korsmeyer-Peppas with diffusion exponent (n) values related to non-Fickian transport behavior suggesting a combination of erosion and diffusion mechanisms.
32294500	3	6	theme	maximum	618:624	arg1	loading					626:632	420 mg/g maximum loading	609:632	420 mg/g maximum loading	609:632	The investigated composites are of 328 mg/g, 360 mg/g, 479 mg/g, and 420 mg/g maximum loading capacities for MCM-48, MCM-48/ST, MCM-48/CH, and MCM-48/β-CD, respectively.
32294500	0	7	theme	pharmacokinetic	168:182	arg1	properties					184:193	pharmacokinetic properties	168:193	pharmacokinetic properties	168:193	Insight into the role of integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin) with mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties.
32294500	9	8	theme	results	1486:1492	arg1	investigation					1455:1467	The pharmacokinetic investigation	1435:1467	The pharmacokinetic investigation of the releasing results	1435:1492	The pharmacokinetic investigation of the releasing results demonstrated the best fitting with Korsmeyer-Peppas with diffusion exponent (n) values related to non-Fickian transport behavior suggesting a combination of erosion and diffusion mechanisms.
32294500	6	9	with	fitting	1131:1137	arg1	hypothesis					1157:1166	the Langmuir hypothesis	1144:1166	the Langmuir hypothesis	1144:1166	The equilibrium fitting suggested monolayer loading for ibuprofen by MCM-48 and MCM-48/CH with excellent fitting with the Langmuir hypothesis.
32294500	9	10	theme	fitting	1516:1522	arg1	values					1574:1579	the best fitting with Korsmeyer-Peppas with diffusion exponent (n) values	1507:1579	the best fitting with Korsmeyer-Peppas with diffusion exponent (n) values related to non-Fickian transport behavior	1507:1621	The pharmacokinetic investigation of the releasing results demonstrated the best fitting with Korsmeyer-Peppas with diffusion exponent (n) values related to non-Fickian transport behavior suggesting a combination of erosion and diffusion mechanisms.
32294500	9	11	theme	erosion	1651:1657	arg1	combination					1636:1646	a combination	1634:1646	a combination of erosion and diffusion mechanisms	1634:1682	The pharmacokinetic investigation of the releasing results demonstrated the best fitting with Korsmeyer-Peppas with diffusion exponent (n) values related to non-Fickian transport behavior suggesting a combination of erosion and diffusion mechanisms.
32294500	1	12	dep	nanocomposites	355:368	arg1	MCM-48/β-CD					393:403	MCM-48/β-CD	393:403	MCM-48/β-CD	393:403	Three types of carbohydrate polymers starch (ST), chitosan (CH), and β-cyclodextrin (β-CD) were integrated with mesoporous silica (MCM-48) in some biopolymers nanocomposites (MCM-48/ST, MCM-48/CH, MCM-48/β-CD) as promising delivery systems for ibuprofen drug.
32294500	1	12	dep	nanocomposites	355:368	arg1	MCM-48/CH					382:390	MCM-48/CH	382:390	MCM-48/CH	382:390	Three types of carbohydrate polymers starch (ST), chitosan (CH), and β-cyclodextrin (β-CD) were integrated with mesoporous silica (MCM-48) in some biopolymers nanocomposites (MCM-48/ST, MCM-48/CH, MCM-48/β-CD) as promising delivery systems for ibuprofen drug.
32294500	1	12	dep	nanocomposites	355:368	arg1	MCM-48/ST					371:379	MCM-48/ST	371:379	MCM-48/ST	371:379	Three types of carbohydrate polymers starch (ST), chitosan (CH), and β-cyclodextrin (β-CD) were integrated with mesoporous silica (MCM-48) in some biopolymers nanocomposites (MCM-48/ST, MCM-48/CH, MCM-48/β-CD) as promising delivery systems for ibuprofen drug.
32294500	1	13	theme	mesoporous	308:317	arg1	MCM-48					327:332	MCM-48	327:332	MCM-48	327:332	Three types of carbohydrate polymers starch (ST), chitosan (CH), and β-cyclodextrin (β-CD) were integrated with mesoporous silica (MCM-48) in some biopolymers nanocomposites (MCM-48/ST, MCM-48/CH, MCM-48/β-CD) as promising delivery systems for ibuprofen drug.
32294500	1	13	theme	mesoporous	308:317	arg1	silica					319:324	mesoporous silica	308:324	mesoporous silica (MCM-48) in some biopolymers nanocomposites (MCM-48/ST, MCM-48/CH, MCM-48/β-CD) as promising delivery systems for ibuprofen drug	308:453	Three types of carbohydrate polymers starch (ST), chitosan (CH), and β-cyclodextrin (β-CD) were integrated with mesoporous silica (MCM-48) in some biopolymers nanocomposites (MCM-48/ST, MCM-48/CH, MCM-48/β-CD) as promising delivery systems for ibuprofen drug.
32294500	8	14	theme	releasing	1286:1294	arg1	profiles					1296:1303	The releasing profiles	1282:1303	The releasing profiles of the drug	1282:1315	The releasing profiles of the drug reflected significant controlled properties depending on the ratio and the type of the integrated polymers for 300 h.
32294500	4	15	theme	order	771:775	arg1	model					777:781	the Pseudo-first order model	754:781	the Pseudo-first order model suggesting physisorption loading mechanisms	754:825	The kinetic studies revealed preference for the Pseudo-first order model suggesting physisorption loading mechanisms.
32294500	7	16	theme	Freundlich	1259:1268	arg1	assumption					1270:1279	the Freundlich assumption	1255:1279	the Freundlich assumption	1255:1279	The loading properties of MCM-48/ST and MCM-48/β-CD are of multilayer form and follow the Freundlich assumption.
32294500	1	17	from	silica	319:324	arg1	nanocomposites					355:368	some biopolymers nanocomposites	338:368	some biopolymers nanocomposites (MCM-48/ST, MCM-48/CH, MCM-48/β-CD)	338:404	Three types of carbohydrate polymers starch (ST), chitosan (CH), and β-cyclodextrin (β-CD) were integrated with mesoporous silica (MCM-48) in some biopolymers nanocomposites (MCM-48/ST, MCM-48/CH, MCM-48/β-CD) as promising delivery systems for ibuprofen drug.
32294500	4	18	theme	physisorption	794:806	arg1	mechanisms					816:825	physisorption loading mechanisms	794:825	physisorption loading mechanisms	794:825	The kinetic studies revealed preference for the Pseudo-first order model suggesting physisorption loading mechanisms.
32294500	5	19	theme	adsorption	894:903	arg1	KJ/mol					928:933	0.55 KJ/mol	923:933	0.55 KJ/mol (MCM-48)	923:942	This supported by the thermodynamic parameters and the recognized adsorption energies that are 0.55 KJ/mol (MCM-48), 0.63 KJ/mol (MCM-48/ST), 0.65 KJ/mol (MCM-48/CH), and 0.65 KJ/mol (MCM-48/β-CD).
32294500	5	19	theme	adsorption	894:903	arg1	energies					905:912	the recognized adsorption energies	879:912	the recognized adsorption energies that are 0.55 KJ/mol (MCM-48), 0.63 KJ/mol (MCM-48/ST), 0.65 KJ/mol (MCM-48/CH), and 0.65 KJ/mol (MCM-48/β-CD)	879:1023	This supported by the thermodynamic parameters and the recognized adsorption energies that are 0.55 KJ/mol (MCM-48), 0.63 KJ/mol (MCM-48/ST), 0.65 KJ/mol (MCM-48/CH), and 0.65 KJ/mol (MCM-48/β-CD).
32294500	7	20	theme	MCM-48/ST	1195:1203	arg1	properties					1181:1190	The loading properties	1169:1190	The loading properties of MCM-48/ST and MCM-48/β-CD	1169:1219	The loading properties of MCM-48/ST and MCM-48/β-CD are of multilayer form and follow the Freundlich assumption.
32294500	9	21	theme	diffusion	1551:1559	arg1	exponent					1561:1568	diffusion exponent	1551:1568	diffusion exponent	1551:1568	The pharmacokinetic investigation of the releasing results demonstrated the best fitting with Korsmeyer-Peppas with diffusion exponent (n) values related to non-Fickian transport behavior suggesting a combination of erosion and diffusion mechanisms.
32294500	1	22	theme	chitosan	246:253	arg1	types					202:206	Three types	196:206	Three types of carbohydrate polymers starch (ST), chitosan (CH), and β-cyclodextrin (β-CD)	196:285	Three types of carbohydrate polymers starch (ST), chitosan (CH), and β-cyclodextrin (β-CD) were integrated with mesoporous silica (MCM-48) in some biopolymers nanocomposites (MCM-48/ST, MCM-48/CH, MCM-48/β-CD) as promising delivery systems for ibuprofen drug.
32294500	8	23	theme	polymers	1415:1422	arg1	type					1392:1395	the type	1388:1395	the type	1388:1395	The releasing profiles of the drug reflected significant controlled properties depending on the ratio and the type of the integrated polymers for 300 h.
32294500	8	23	theme	polymers	1415:1422	arg1	ratio					1378:1382	ratio	1378:1382	ratio	1378:1382	The releasing profiles of the drug reflected significant controlled properties depending on the ratio and the type of the integrated polymers for 300 h.
32294500	1	24	theme	promising	409:417	arg1	systems					428:434	promising delivery systems	409:434	promising delivery systems for ibuprofen drug	409:453	Three types of carbohydrate polymers starch (ST), chitosan (CH), and β-cyclodextrin (β-CD) were integrated with mesoporous silica (MCM-48) in some biopolymers nanocomposites (MCM-48/ST, MCM-48/CH, MCM-48/β-CD) as promising delivery systems for ibuprofen drug.
32294500	8	25	theme	integrated	1404:1413	arg1	polymers					1415:1422	the integrated polymers	1400:1422	the integrated polymers	1400:1422	The releasing profiles of the drug reflected significant controlled properties depending on the ratio and the type of the integrated polymers for 300 h.
32294500	3	26	theme	360 mg/g	585:592	arg1	capacities					634:643	328 mg/g, 360 mg/g, 479 mg/g, and 420 mg/g maximum loading capacities	575:643	328 mg/g, 360 mg/g, 479 mg/g, and 420 mg/g maximum loading capacities for MCM-48, MCM-48/ST, MCM-48/CH, and MCM-48/β-CD, respectively	575:707	The investigated composites are of 328 mg/g, 360 mg/g, 479 mg/g, and 420 mg/g maximum loading capacities for MCM-48, MCM-48/ST, MCM-48/CH, and MCM-48/β-CD, respectively.
32294500	9	27	with	fitting	1516:1522	arg1	Korsmeyer-Peppas					1529:1544	Korsmeyer-Peppas	1529:1544	Korsmeyer-Peppas with diffusion exponent	1529:1568	The pharmacokinetic investigation of the releasing results demonstrated the best fitting with Korsmeyer-Peppas with diffusion exponent (n) values related to non-Fickian transport behavior suggesting a combination of erosion and diffusion mechanisms.
32294500	0	28	theme	mesoporous	102:111	arg1	silica					113:118	mesoporous silica	102:118	mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties	102:193	Insight into the role of integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin) with mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties.
32294500	1	29	theme	delivery	419:426	arg1	systems					428:434	promising delivery systems	409:434	promising delivery systems for ibuprofen drug	409:453	Three types of carbohydrate polymers starch (ST), chitosan (CH), and β-cyclodextrin (β-CD) were integrated with mesoporous silica (MCM-48) in some biopolymers nanocomposites (MCM-48/ST, MCM-48/CH, MCM-48/β-CD) as promising delivery systems for ibuprofen drug.
32294500	6	30	theme	monolayer	1060:1068	arg1	loading					1070:1076	monolayer loading	1060:1076	monolayer loading for ibuprofen by MCM-48 and MCM-48/CH	1060:1114	The equilibrium fitting suggested monolayer loading for ibuprofen by MCM-48 and MCM-48/CH with excellent fitting with the Langmuir hypothesis.
32294500	8	31	theme	significant	1327:1337	arg1	properties					1350:1359	significant controlled properties	1327:1359	significant controlled properties	1327:1359	The releasing profiles of the drug reflected significant controlled properties depending on the ratio and the type of the integrated polymers for 300 h.
32294500	7	32	theme	multilayer	1228:1237	arg1	form					1239:1242	multilayer form	1228:1242	multilayer form	1228:1242	The loading properties of MCM-48/ST and MCM-48/β-CD are of multilayer form and follow the Freundlich assumption.
32294500	9	33	theme	diffusion	1663:1671	arg1	combination					1636:1646	a combination	1634:1646	a combination of erosion and diffusion mechanisms	1634:1682	The pharmacokinetic investigation of the releasing results demonstrated the best fitting with Korsmeyer-Peppas with diffusion exponent (n) values related to non-Fickian transport behavior suggesting a combination of erosion and diffusion mechanisms.
32294500	9	34	theme	releasing	1476:1484	arg1	results					1486:1492	the releasing results	1472:1492	the releasing results	1472:1492	The pharmacokinetic investigation of the releasing results demonstrated the best fitting with Korsmeyer-Peppas with diffusion exponent (n) values related to non-Fickian transport behavior suggesting a combination of erosion and diffusion mechanisms.
32294500	0	35	theme	integrated	25:34	arg1	chitosan					67:74	chitosan	67:74	chitosan	67:74	Insight into the role of integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin) with mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties.
32294500	0	35	theme	integrated	25:34	arg1	β-cyclodextrin					81:94	β-cyclodextrin	81:94	β-cyclodextrin	81:94	Insight into the role of integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin) with mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties.
32294500	0	35	theme	integrated	25:34	arg1	starch					59:64	starch	59:64	starch	59:64	Insight into the role of integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin) with mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties.
32294500	0	35	theme	integrated	25:34	arg1	polymers					49:56	integrated carbohydrate polymers	25:56	integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin)	25:95	Insight into the role of integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin) with mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties.
32294500	7	36	theme	MCM-48/β-CD	1209:1219	arg1	properties					1181:1190	The loading properties	1169:1190	The loading properties of MCM-48/ST and MCM-48/β-CD	1169:1219	The loading properties of MCM-48/ST and MCM-48/β-CD are of multilayer form and follow the Freundlich assumption.
32294500	2	37	theme	simple	478:483	arg1	mixing					485:490	simple mixing	478:490	simple mixing of the polymers gels with the MCM-48 fractions	478:537	This was conducted by simple mixing of the polymers gels with the MCM-48 fractions.
32294500	1	38	dep	starch	233:238	arg1	ST					241:242	ST	241:242	ST	241:242	Three types of carbohydrate polymers starch (ST), chitosan (CH), and β-cyclodextrin (β-CD) were integrated with mesoporous silica (MCM-48) in some biopolymers nanocomposites (MCM-48/ST, MCM-48/CH, MCM-48/β-CD) as promising delivery systems for ibuprofen drug.
32294500	0	39	dep	polymers	49:56	arg1	chitosan					67:74	chitosan	67:74	chitosan	67:74	Insight into the role of integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin) with mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties.
32294500	0	39	dep	polymers	49:56	arg1	β-cyclodextrin					81:94	β-cyclodextrin	81:94	β-cyclodextrin	81:94	Insight into the role of integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin) with mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties.
32294500	0	39	dep	polymers	49:56	arg1	starch					59:64	starch	59:64	starch	59:64	Insight into the role of integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin) with mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties.
32294500	0	39	dep	polymers	49:56	arg1	polymers					49:56	integrated carbohydrate polymers	25:56	integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin)	25:95	Insight into the role of integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin) with mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties.
32294500	9	40	dep	erosion	1651:1657	arg1	mechanisms					1673:1682	mechanisms	1673:1682	mechanisms	1673:1682	The pharmacokinetic investigation of the releasing results demonstrated the best fitting with Korsmeyer-Peppas with diffusion exponent (n) values related to non-Fickian transport behavior suggesting a combination of erosion and diffusion mechanisms.
32294500	6	41	theme	Langmuir	1148:1155	arg1	hypothesis					1157:1166	the Langmuir hypothesis	1144:1166	the Langmuir hypothesis	1144:1166	The equilibrium fitting suggested monolayer loading for ibuprofen by MCM-48 and MCM-48/CH with excellent fitting with the Langmuir hypothesis.
32294500	0	42	with	role	17:20	arg1	silica					113:118	mesoporous silica	102:118	mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties	102:193	Insight into the role of integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin) with mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties.
32294500	0	43	theme	polymers	49:56	arg1	role					17:20	the role	13:20	the role of integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin) with mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties	13:193	Insight into the role of integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin) with mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties.
32294500	3	44	theme	328 mg/g	575:582	arg1	capacities					634:643	328 mg/g, 360 mg/g, 479 mg/g, and 420 mg/g maximum loading capacities	575:643	328 mg/g, 360 mg/g, 479 mg/g, and 420 mg/g maximum loading capacities for MCM-48, MCM-48/ST, MCM-48/CH, and MCM-48/β-CD, respectively	575:707	The investigated composites are of 328 mg/g, 360 mg/g, 479 mg/g, and 420 mg/g maximum loading capacities for MCM-48, MCM-48/ST, MCM-48/CH, and MCM-48/β-CD, respectively.
32294500	4	45	theme	Pseudo-first	758:769	arg1	model					777:781	the Pseudo-first order model	754:781	the Pseudo-first order model suggesting physisorption loading mechanisms	754:825	The kinetic studies revealed preference for the Pseudo-first order model suggesting physisorption loading mechanisms.
32294500	2	46	theme	polymers	499:506	arg1	gels					508:511	the polymers gels	495:511	the polymers gels	495:511	This was conducted by simple mixing of the polymers gels with the MCM-48 fractions.
32294500	8	47	dep	ratio	1378:1382	arg1	the					1374:1376	the	1374:1376	the	1374:1376	The releasing profiles of the drug reflected significant controlled properties depending on the ratio and the type of the integrated polymers for 300 h.
32294500	0	48	theme	carbohydrate	36:47	arg1	chitosan					67:74	chitosan	67:74	chitosan	67:74	Insight into the role of integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin) with mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties.
32294500	0	48	theme	carbohydrate	36:47	arg1	β-cyclodextrin					81:94	β-cyclodextrin	81:94	β-cyclodextrin	81:94	Insight into the role of integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin) with mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties.
32294500	0	48	theme	carbohydrate	36:47	arg1	starch					59:64	starch	59:64	starch	59:64	Insight into the role of integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin) with mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties.
32294500	0	48	theme	carbohydrate	36:47	arg1	polymers					49:56	integrated carbohydrate polymers	25:56	integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin)	25:95	Insight into the role of integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin) with mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties.
32294500	1	49	theme	β-cyclodextrin	265:278	arg1	types					202:206	Three types	196:206	Three types of carbohydrate polymers starch (ST), chitosan (CH), and β-cyclodextrin (β-CD)	196:285	Three types of carbohydrate polymers starch (ST), chitosan (CH), and β-cyclodextrin (β-CD) were integrated with mesoporous silica (MCM-48) in some biopolymers nanocomposites (MCM-48/ST, MCM-48/CH, MCM-48/β-CD) as promising delivery systems for ibuprofen drug.
32294500	2	50	theme	gels	508:511	arg1	mixing					485:490	simple mixing	478:490	simple mixing of the polymers gels with the MCM-48 fractions	478:537	This was conducted by simple mixing of the polymers gels with the MCM-48 fractions.
32294500	1	51	theme	biopolymers	343:353	arg1	nanocomposites					355:368	some biopolymers nanocomposites	338:368	some biopolymers nanocomposites (MCM-48/ST, MCM-48/CH, MCM-48/β-CD)	338:404	Three types of carbohydrate polymers starch (ST), chitosan (CH), and β-cyclodextrin (β-CD) were integrated with mesoporous silica (MCM-48) in some biopolymers nanocomposites (MCM-48/ST, MCM-48/CH, MCM-48/β-CD) as promising delivery systems for ibuprofen drug.
32294500	8	52	theme	controlled	1339:1348	arg1	properties					1350:1359	significant controlled properties	1327:1359	significant controlled properties	1327:1359	The releasing profiles of the drug reflected significant controlled properties depending on the ratio and the type of the integrated polymers for 300 h.
32294500	1	53	theme	ibuprofen	440:448	arg1	drug					450:453	ibuprofen drug	440:453	ibuprofen drug	440:453	Three types of carbohydrate polymers starch (ST), chitosan (CH), and β-cyclodextrin (β-CD) were integrated with mesoporous silica (MCM-48) in some biopolymers nanocomposites (MCM-48/ST, MCM-48/CH, MCM-48/β-CD) as promising delivery systems for ibuprofen drug.
32294500	6	54	theme	equilibrium	1030:1040	arg1	fitting					1042:1048	The equilibrium fitting	1026:1048	The equilibrium fitting	1026:1048	The equilibrium fitting suggested monolayer loading for ibuprofen by MCM-48 and MCM-48/CH with excellent fitting with the Langmuir hypothesis.
32294500	0	55	theme	drug	146:149	arg1	equilibrium					152:162	ibuprofen drug; equilibrium	136:162	ibuprofen drug; equilibrium	136:162	Insight into the role of integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin) with mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties.
32294500	4	56	theme	kinetic	714:720	arg1	studies					722:728	The kinetic studies	710:728	The kinetic studies	710:728	The kinetic studies revealed preference for the Pseudo-first order model suggesting physisorption loading mechanisms.
32294500	8	57	theme	drug	1312:1315	arg1	profiles					1296:1303	The releasing profiles	1282:1303	The releasing profiles of the drug	1282:1315	The releasing profiles of the drug reflected significant controlled properties depending on the ratio and the type of the integrated polymers for 300 h.
32294500	0	58	theme	ibuprofen	136:144	arg1	equilibrium					152:162	ibuprofen drug; equilibrium	136:162	ibuprofen drug; equilibrium	136:162	Insight into the role of integrated carbohydrate polymers (starch, chitosan, and β-cyclodextrin) with mesoporous silica as carriers for ibuprofen drug; equilibrium and pharmacokinetic properties.
32294500	6	59	theme	excellent	1121:1129	arg1	fitting					1131:1137	excellent fitting	1121:1137	excellent fitting with the Langmuir hypothesis	1121:1166	The equilibrium fitting suggested monolayer loading for ibuprofen by MCM-48 and MCM-48/CH with excellent fitting with the Langmuir hypothesis.
32294500	5	60	theme	recognized	883:892	arg1	KJ/mol					928:933	0.55 KJ/mol	923:933	0.55 KJ/mol (MCM-48)	923:942	This supported by the thermodynamic parameters and the recognized adsorption energies that are 0.55 KJ/mol (MCM-48), 0.63 KJ/mol (MCM-48/ST), 0.65 KJ/mol (MCM-48/CH), and 0.65 KJ/mol (MCM-48/β-CD).
32294500	5	60	theme	recognized	883:892	arg1	energies					905:912	the recognized adsorption energies	879:912	the recognized adsorption energies that are 0.55 KJ/mol (MCM-48), 0.63 KJ/mol (MCM-48/ST), 0.65 KJ/mol (MCM-48/CH), and 0.65 KJ/mol (MCM-48/β-CD)	879:1023	This supported by the thermodynamic parameters and the recognized adsorption energies that are 0.55 KJ/mol (MCM-48), 0.63 KJ/mol (MCM-48/ST), 0.65 KJ/mol (MCM-48/CH), and 0.65 KJ/mol (MCM-48/β-CD).
32294500	1	61	theme	carbohydrate	211:222	arg1	polymers					224:231	carbohydrate polymers	211:231	carbohydrate polymers starch (ST)	211:243	Three types of carbohydrate polymers starch (ST), chitosan (CH), and β-cyclodextrin (β-CD) were integrated with mesoporous silica (MCM-48) in some biopolymers nanocomposites (MCM-48/ST, MCM-48/CH, MCM-48/β-CD) as promising delivery systems for ibuprofen drug.
32294500	3	62	theme	479 mg/g	595:602	arg1	capacities					634:643	328 mg/g, 360 mg/g, 479 mg/g, and 420 mg/g maximum loading capacities	575:643	328 mg/g, 360 mg/g, 479 mg/g, and 420 mg/g maximum loading capacities for MCM-48, MCM-48/ST, MCM-48/CH, and MCM-48/β-CD, respectively	575:707	The investigated composites are of 328 mg/g, 360 mg/g, 479 mg/g, and 420 mg/g maximum loading capacities for MCM-48, MCM-48/ST, MCM-48/CH, and MCM-48/β-CD, respectively.
32294500	9	63	theme	related	1581:1587	arg1	values					1574:1579	the best fitting with Korsmeyer-Peppas with diffusion exponent (n) values	1507:1579	the best fitting with Korsmeyer-Peppas with diffusion exponent (n) values related to non-Fickian transport behavior	1507:1621	The pharmacokinetic investigation of the releasing results demonstrated the best fitting with Korsmeyer-Peppas with diffusion exponent (n) values related to non-Fickian transport behavior suggesting a combination of erosion and diffusion mechanisms.
32294500	7	64	theme	loading	1173:1179	arg1	properties					1181:1190	The loading properties	1169:1190	The loading properties of MCM-48/ST and MCM-48/β-CD	1169:1219	The loading properties of MCM-48/ST and MCM-48/β-CD are of multilayer form and follow the Freundlich assumption.
32294500	2	65	with	mixing	485:490	arg1	fractions					529:537	the MCM-48 fractions	518:537	the MCM-48 fractions	518:537	This was conducted by simple mixing of the polymers gels with the MCM-48 fractions.
32294500	1	66	theme	polymers	224:231	arg1	starch					233:238	carbohydrate polymers starch	211:238	carbohydrate polymers starch (ST)	211:243	Three types of carbohydrate polymers starch (ST), chitosan (CH), and β-cyclodextrin (β-CD) were integrated with mesoporous silica (MCM-48) in some biopolymers nanocomposites (MCM-48/ST, MCM-48/CH, MCM-48/β-CD) as promising delivery systems for ibuprofen drug.
32294500	9	67	theme	pharmacokinetic	1439:1453	arg1	investigation					1455:1467	The pharmacokinetic investigation	1435:1467	The pharmacokinetic investigation of the releasing results	1435:1492	The pharmacokinetic investigation of the releasing results demonstrated the best fitting with Korsmeyer-Peppas with diffusion exponent (n) values related to non-Fickian transport behavior suggesting a combination of erosion and diffusion mechanisms.
32294500	3	68	theme	loading	626:632	arg1	capacities					634:643	328 mg/g, 360 mg/g, 479 mg/g, and 420 mg/g maximum loading capacities	575:643	328 mg/g, 360 mg/g, 479 mg/g, and 420 mg/g maximum loading capacities for MCM-48, MCM-48/ST, MCM-48/CH, and MCM-48/β-CD, respectively	575:707	The investigated composites are of 328 mg/g, 360 mg/g, 479 mg/g, and 420 mg/g maximum loading capacities for MCM-48, MCM-48/ST, MCM-48/CH, and MCM-48/β-CD, respectively.
32294500	3	69	theme	investigated	544:555	arg1	composites					557:566	The investigated composites	540:566	The investigated composites	540:566	The investigated composites are of 328 mg/g, 360 mg/g, 479 mg/g, and 420 mg/g maximum loading capacities for MCM-48, MCM-48/ST, MCM-48/CH, and MCM-48/β-CD, respectively.
32294500	9	70	with	Korsmeyer-Peppas	1529:1544	arg1	exponent					1561:1568	diffusion exponent	1551:1568	diffusion exponent	1551:1568	The pharmacokinetic investigation of the releasing results demonstrated the best fitting with Korsmeyer-Peppas with diffusion exponent (n) values related to non-Fickian transport behavior suggesting a combination of erosion and diffusion mechanisms.
32294500	1	71	theme	starch	233:238	arg1	types					202:206	Three types	196:206	Three types of carbohydrate polymers starch (ST), chitosan (CH), and β-cyclodextrin (β-CD)	196:285	Three types of carbohydrate polymers starch (ST), chitosan (CH), and β-cyclodextrin (β-CD) were integrated with mesoporous silica (MCM-48) in some biopolymers nanocomposites (MCM-48/ST, MCM-48/CH, MCM-48/β-CD) as promising delivery systems for ibuprofen drug.
34755459	0	0	theme	Nanoparticles	106:118	arg1	Hydrogels					10:18	Composite Hydrogels	0:18	Composite Hydrogels of Alkyl Functionalized Gellan Gum Derivative and Hydroxyapatite/Tricalcium Phosphate Nanoparticles as Injectable Scaffolds for bone Regeneration.	0:165	Composite Hydrogels of Alkyl Functionalized Gellan Gum Derivative and Hydroxyapatite/Tricalcium Phosphate Nanoparticles as Injectable Scaffolds for bone Regeneration.
34755459	6	1	theme	release	1214:1220	arg1	experiments					1222:1232	Loading and release experiments	1202:1232	Loading and release experiments for dexamethasone and stromal cell-derived factor-1	1202:1284	Loading and release experiments for dexamethasone and stromal cell-derived factor-1 demonstrate the drug delivery features of the obtained injectable systems.
34755459	4	2	theme	inorganic	813:821	arg1	nanoparticles					823:835	inorganic nanoparticles	813:835	inorganic nanoparticles	813:835	It is found that the presence of inorganic nanoparticles reinforces the 3D hydrated polymeric networks without influencing their injectability but improving the physicochemical properties of ionotropic crosslinked hydrogels produced with two different curing media.
34755459	6	3	theme	stromal	1256:1262	arg1	factor-1					1277:1284	stromal cell-derived factor-1	1256:1284	stromal cell-derived factor-1	1256:1284	Loading and release experiments for dexamethasone and stromal cell-derived factor-1 demonstrate the drug delivery features of the obtained injectable systems.
34755459	1	4	theme	gellan	191:196	arg1	gum					198:200	An alkyl functionalized gellan gum	167:200	An alkyl functionalized gellan gum derivative	167:211	An alkyl functionalized gellan gum derivative is here used to produce hydrogels containing hydroxyapatite and tricalcium phosphate nanoparticles as injectable nanostructured scaffolds for bone regeneration.
34755459	6	5	theme	Loading	1202:1208	arg1	experiments					1222:1232	Loading and release experiments	1202:1232	Loading and release experiments for dexamethasone and stromal cell-derived factor-1	1202:1284	Loading and release experiments for dexamethasone and stromal cell-derived factor-1 demonstrate the drug delivery features of the obtained injectable systems.
34755459	0	6	theme	Phosphate	96:104	arg1	Nanoparticles					106:118	Alkyl Functionalized Gellan Gum Derivative and Hydroxyapatite/Tricalcium Phosphate Nanoparticles	23:118	Alkyl Functionalized Gellan Gum Derivative and Hydroxyapatite/Tricalcium Phosphate Nanoparticles as Injectable Scaffolds for bone Regeneration	23:164	Composite Hydrogels of Alkyl Functionalized Gellan Gum Derivative and Hydroxyapatite/Tricalcium Phosphate Nanoparticles as Injectable Scaffolds for bone Regeneration.
34755459	4	7	theme	curing	1032:1037	arg1	media					1039:1043	two different curing media	1018:1043	two different curing media	1018:1043	It is found that the presence of inorganic nanoparticles reinforces the 3D hydrated polymeric networks without influencing their injectability but improving the physicochemical properties of ionotropic crosslinked hydrogels produced with two different curing media.
34755459	1	8	theme	gum	198:200	arg1	derivative					202:211	An alkyl functionalized gellan gum derivative	167:211	An alkyl functionalized gellan gum derivative	167:211	An alkyl functionalized gellan gum derivative is here used to produce hydrogels containing hydroxyapatite and tricalcium phosphate nanoparticles as injectable nanostructured scaffolds for bone regeneration.
34755459	3	9	from	influence	641:649	arg1	properties					731:740	the rheological and physicochemical properties	695:740	the rheological and physicochemical properties of the injectable systems	695:766	The influence of different nanoparticles concentration on the rheological and physicochemical properties of the injectable systems is studied.
34755459	0	10	theme	Injectable	123:132	arg1	Scaffolds					134:142	Injectable Scaffolds	123:142	Injectable Scaffolds for bone Regeneration	123:164	Composite Hydrogels of Alkyl Functionalized Gellan Gum Derivative and Hydroxyapatite/Tricalcium Phosphate Nanoparticles as Injectable Scaffolds for bone Regeneration.
34755459	5	11	theme	based	1149:1153	arg1	hydrogels					1155:1163	gellan gum based hydrogels	1138:1163	gellan gum based hydrogels	1138:1163	Preliminary cytocompatibility tests performed with murine preosteoblast cells revealed that gellan gum based hydrogels can safely encapsulate viable cells.
34755459	1	12	theme	injectable	315:324	arg1	scaffolds					341:349	injectable nanostructured scaffolds	315:349	injectable nanostructured scaffolds for bone regeneration	315:371	An alkyl functionalized gellan gum derivative is here used to produce hydrogels containing hydroxyapatite and tricalcium phosphate nanoparticles as injectable nanostructured scaffolds for bone regeneration.
34755459	2	13	theme	osteoinductive	608:621	arg1	biomolecules					623:634	osteoinductive biomolecules	608:634	osteoinductive biomolecules	608:634	The amphiphilic nature of the polysaccharide derivative along with its thermotropic behavior and ionotropic crosslinking features make possible to produce injectable bone mimetic scaffolds that can be used to release viable cells and osteoinductive biomolecules.
34755459	2	14	dep	along	430:434	arg1	with					436:439	with	436:439	with	436:439	The amphiphilic nature of the polysaccharide derivative along with its thermotropic behavior and ionotropic crosslinking features make possible to produce injectable bone mimetic scaffolds that can be used to release viable cells and osteoinductive biomolecules.
34755459	1	15	theme	nanostructured	326:339	arg1	scaffolds					341:349	injectable nanostructured scaffolds	315:349	injectable nanostructured scaffolds for bone regeneration	315:371	An alkyl functionalized gellan gum derivative is here used to produce hydrogels containing hydroxyapatite and tricalcium phosphate nanoparticles as injectable nanostructured scaffolds for bone regeneration.
34755459	5	16	theme	murine	1097:1102	arg1	cells					1118:1122	murine preosteoblast cells	1097:1122	murine preosteoblast cells	1097:1122	Preliminary cytocompatibility tests performed with murine preosteoblast cells revealed that gellan gum based hydrogels can safely encapsulate viable cells.
34755459	5	17	theme	gellan	1138:1143	arg1	hydrogels					1155:1163	gellan gum based hydrogels	1138:1163	gellan gum based hydrogels	1138:1163	Preliminary cytocompatibility tests performed with murine preosteoblast cells revealed that gellan gum based hydrogels can safely encapsulate viable cells.
34755459	4	18	theme	polymeric	864:872	arg1	networks					874:881	the 3D hydrated polymeric networks	848:881	the 3D hydrated polymeric networks	848:881	It is found that the presence of inorganic nanoparticles reinforces the 3D hydrated polymeric networks without influencing their injectability but improving the physicochemical properties of ionotropic crosslinked hydrogels produced with two different curing media.
34755459	2	19	theme	crosslinking	482:493	arg1	features					495:502	ionotropic crosslinking features	471:502	ionotropic crosslinking features	471:502	The amphiphilic nature of the polysaccharide derivative along with its thermotropic behavior and ionotropic crosslinking features make possible to produce injectable bone mimetic scaffolds that can be used to release viable cells and osteoinductive biomolecules.
34755459	6	20	link	cell-derived	1264:1275	arg1	factor-1					1277:1284	stromal cell-derived factor-1	1256:1284	stromal cell-derived factor-1	1256:1284	Loading and release experiments for dexamethasone and stromal cell-derived factor-1 demonstrate the drug delivery features of the obtained injectable systems.
34755459	6	21	theme	drug	1302:1305	arg1	features					1316:1323	the drug delivery features	1298:1323	the drug delivery features of the obtained injectable systems	1298:1358	Loading and release experiments for dexamethasone and stromal cell-derived factor-1 demonstrate the drug delivery features of the obtained injectable systems.
34755459	2	22	theme	bone	540:543	arg1	scaffolds					553:561	injectable bone mimetic scaffolds	529:561	injectable bone mimetic scaffolds that can be used to release viable cells and osteoinductive biomolecules	529:634	The amphiphilic nature of the polysaccharide derivative along with its thermotropic behavior and ionotropic crosslinking features make possible to produce injectable bone mimetic scaffolds that can be used to release viable cells and osteoinductive biomolecules.
34755459	6	23	theme	delivery	1307:1314	arg1	features					1316:1323	the drug delivery features	1298:1323	the drug delivery features of the obtained injectable systems	1298:1358	Loading and release experiments for dexamethasone and stromal cell-derived factor-1 demonstrate the drug delivery features of the obtained injectable systems.
34755459	2	24	theme	ionotropic	471:480	arg1	features					495:502	ionotropic crosslinking features	471:502	ionotropic crosslinking features	471:502	The amphiphilic nature of the polysaccharide derivative along with its thermotropic behavior and ionotropic crosslinking features make possible to produce injectable bone mimetic scaffolds that can be used to release viable cells and osteoinductive biomolecules.
34755459	2	25	theme	mimetic	545:551	arg1	scaffolds					553:561	injectable bone mimetic scaffolds	529:561	injectable bone mimetic scaffolds that can be used to release viable cells and osteoinductive biomolecules	529:634	The amphiphilic nature of the polysaccharide derivative along with its thermotropic behavior and ionotropic crosslinking features make possible to produce injectable bone mimetic scaffolds that can be used to release viable cells and osteoinductive biomolecules.
34755459	4	26	theme	different	1022:1030	arg1	media					1039:1043	two different curing media	1018:1043	two different curing media	1018:1043	It is found that the presence of inorganic nanoparticles reinforces the 3D hydrated polymeric networks without influencing their injectability but improving the physicochemical properties of ionotropic crosslinked hydrogels produced with two different curing media.
34755459	2	27	theme	viable	591:596	arg1	cells					598:602	viable cells	591:602	viable cells	591:602	The amphiphilic nature of the polysaccharide derivative along with its thermotropic behavior and ionotropic crosslinking features make possible to produce injectable bone mimetic scaffolds that can be used to release viable cells and osteoinductive biomolecules.
34755459	3	28	theme	concentration	678:690	arg1	influence					641:649	The influence	637:649	The influence of different nanoparticles concentration on the rheological and physicochemical properties of the injectable systems	637:766	The influence of different nanoparticles concentration on the rheological and physicochemical properties of the injectable systems is studied.
34755459	0	29	theme	Composite	0:8	arg1	Hydrogels					10:18	Composite Hydrogels	0:18	Composite Hydrogels of Alkyl Functionalized Gellan Gum Derivative and Hydroxyapatite/Tricalcium Phosphate Nanoparticles as Injectable Scaffolds for bone Regeneration.	0:165	Composite Hydrogels of Alkyl Functionalized Gellan Gum Derivative and Hydroxyapatite/Tricalcium Phosphate Nanoparticles as Injectable Scaffolds for bone Regeneration.
34755459	2	30	used	used	575:578	arg2	scaffolds					553:561	injectable bone mimetic scaffolds	529:561	injectable bone mimetic scaffolds that can be used to release viable cells and osteoinductive biomolecules	529:634	The amphiphilic nature of the polysaccharide derivative along with its thermotropic behavior and ionotropic crosslinking features make possible to produce injectable bone mimetic scaffolds that can be used to release viable cells and osteoinductive biomolecules.
34755459	5	31	theme	Preliminary	1046:1056	arg1	tests					1076:1080	Preliminary cytocompatibility tests	1046:1080	Preliminary cytocompatibility tests performed with murine preosteoblast cells	1046:1122	Preliminary cytocompatibility tests performed with murine preosteoblast cells revealed that gellan gum based hydrogels can safely encapsulate viable cells.
34755459	0	32	theme	Alkyl	23:27	arg1	Derivative					55:64	Alkyl Functionalized Gellan Gum Derivative	23:64	Alkyl Functionalized Gellan Gum Derivative	23:64	Composite Hydrogels of Alkyl Functionalized Gellan Gum Derivative and Hydroxyapatite/Tricalcium Phosphate Nanoparticles as Injectable Scaffolds for bone Regeneration.
34755459	4	33	theme	hydrated	855:862	arg1	networks					874:881	the 3D hydrated polymeric networks	848:881	the 3D hydrated polymeric networks	848:881	It is found that the presence of inorganic nanoparticles reinforces the 3D hydrated polymeric networks without influencing their injectability but improving the physicochemical properties of ionotropic crosslinked hydrogels produced with two different curing media.
34755459	4	34	theme	crosslinked	982:992	arg1	hydrogels					994:1002	ionotropic crosslinked hydrogels	971:1002	ionotropic crosslinked hydrogels produced with two different curing media	971:1043	It is found that the presence of inorganic nanoparticles reinforces the 3D hydrated polymeric networks without influencing their injectability but improving the physicochemical properties of ionotropic crosslinked hydrogels produced with two different curing media.
34755459	3	35	theme	different	654:662	arg1	nanoparticles					664:676	different nanoparticles	654:676	different nanoparticles concentration	654:690	The influence of different nanoparticles concentration on the rheological and physicochemical properties of the injectable systems is studied.
34755459	6	36	theme	cell-derived	1264:1275	arg1	factor-1					1277:1284	stromal cell-derived factor-1	1256:1284	stromal cell-derived factor-1	1256:1284	Loading and release experiments for dexamethasone and stromal cell-derived factor-1 demonstrate the drug delivery features of the obtained injectable systems.
34755459	2	37	theme	amphiphilic	378:388	arg1	nature					390:395	The amphiphilic nature	374:395	The amphiphilic nature of the polysaccharide derivative along with its thermotropic behavior and ionotropic crosslinking features	374:502	The amphiphilic nature of the polysaccharide derivative along with its thermotropic behavior and ionotropic crosslinking features make possible to produce injectable bone mimetic scaffolds that can be used to release viable cells and osteoinductive biomolecules.
34755459	1	38	theme	bone	355:358	arg1	regeneration					360:371	bone regeneration	355:371	bone regeneration	355:371	An alkyl functionalized gellan gum derivative is here used to produce hydrogels containing hydroxyapatite and tricalcium phosphate nanoparticles as injectable nanostructured scaffolds for bone regeneration.
34755459	0	39	theme	bone	148:151	arg1	Regeneration					153:164	bone Regeneration	148:164	bone Regeneration	148:164	Composite Hydrogels of Alkyl Functionalized Gellan Gum Derivative and Hydroxyapatite/Tricalcium Phosphate Nanoparticles as Injectable Scaffolds for bone Regeneration.
34755459	3	40	theme	nanoparticles	664:676	arg1	concentration					678:690	different nanoparticles concentration	654:690	different nanoparticles concentration	654:690	The influence of different nanoparticles concentration on the rheological and physicochemical properties of the injectable systems is studied.
34755459	2	41	theme	thermotropic	445:456	arg1	behavior					458:465	its thermotropic behavior	441:465	its thermotropic behavior	441:465	The amphiphilic nature of the polysaccharide derivative along with its thermotropic behavior and ionotropic crosslinking features make possible to produce injectable bone mimetic scaffolds that can be used to release viable cells and osteoinductive biomolecules.
34755459	0	42	theme	Gellan	44:49	arg1	Derivative					55:64	Alkyl Functionalized Gellan Gum Derivative	23:64	Alkyl Functionalized Gellan Gum Derivative	23:64	Composite Hydrogels of Alkyl Functionalized Gellan Gum Derivative and Hydroxyapatite/Tricalcium Phosphate Nanoparticles as Injectable Scaffolds for bone Regeneration.
34755459	4	43	theme	nanoparticles	823:835	arg1	presence					801:808	the presence	797:808	the presence of inorganic nanoparticles	797:835	It is found that the presence of inorganic nanoparticles reinforces the 3D hydrated polymeric networks without influencing their injectability but improving the physicochemical properties of ionotropic crosslinked hydrogels produced with two different curing media.
34755459	6	44	theme	systems	1352:1358	arg1	features					1316:1323	the drug delivery features	1298:1323	the drug delivery features of the obtained injectable systems	1298:1358	Loading and release experiments for dexamethasone and stromal cell-derived factor-1 demonstrate the drug delivery features of the obtained injectable systems.
34755459	4	45	theme	ionotropic	971:980	arg1	hydrogels					994:1002	ionotropic crosslinked hydrogels	971:1002	ionotropic crosslinked hydrogels produced with two different curing media	971:1043	It is found that the presence of inorganic nanoparticles reinforces the 3D hydrated polymeric networks without influencing their injectability but improving the physicochemical properties of ionotropic crosslinked hydrogels produced with two different curing media.
34755459	0	46	theme	Functionalized	29:42	arg1	Derivative					55:64	Alkyl Functionalized Gellan Gum Derivative	23:64	Alkyl Functionalized Gellan Gum Derivative	23:64	Composite Hydrogels of Alkyl Functionalized Gellan Gum Derivative and Hydroxyapatite/Tricalcium Phosphate Nanoparticles as Injectable Scaffolds for bone Regeneration.
34755459	3	47	theme	systems	760:766	arg1	properties					731:740	the rheological and physicochemical properties	695:740	the rheological and physicochemical properties of the injectable systems	695:766	The influence of different nanoparticles concentration on the rheological and physicochemical properties of the injectable systems is studied.
34755459	2	48	theme	injectable	529:538	arg1	scaffolds					553:561	injectable bone mimetic scaffolds	529:561	injectable bone mimetic scaffolds that can be used to release viable cells and osteoinductive biomolecules	529:634	The amphiphilic nature of the polysaccharide derivative along with its thermotropic behavior and ionotropic crosslinking features make possible to produce injectable bone mimetic scaffolds that can be used to release viable cells and osteoinductive biomolecules.
34755459	0	49	theme	Derivative	55:64	arg1	Nanoparticles					106:118	Alkyl Functionalized Gellan Gum Derivative and Hydroxyapatite/Tricalcium Phosphate Nanoparticles	23:118	Alkyl Functionalized Gellan Gum Derivative and Hydroxyapatite/Tricalcium Phosphate Nanoparticles as Injectable Scaffolds for bone Regeneration	23:164	Composite Hydrogels of Alkyl Functionalized Gellan Gum Derivative and Hydroxyapatite/Tricalcium Phosphate Nanoparticles as Injectable Scaffolds for bone Regeneration.
34755459	5	50	theme	cytocompatibility	1058:1074	arg1	tests					1076:1080	Preliminary cytocompatibility tests	1046:1080	Preliminary cytocompatibility tests performed with murine preosteoblast cells	1046:1122	Preliminary cytocompatibility tests performed with murine preosteoblast cells revealed that gellan gum based hydrogels can safely encapsulate viable cells.
34755459	4	51	theme	hydrogels	994:1002	arg1	properties					957:966	the physicochemical properties	937:966	the physicochemical properties of ionotropic crosslinked hydrogels produced with two different curing media	937:1043	It is found that the presence of inorganic nanoparticles reinforces the 3D hydrated polymeric networks without influencing their injectability but improving the physicochemical properties of ionotropic crosslinked hydrogels produced with two different curing media.
34755459	2	52	theme	derivative	419:428	arg1	nature					390:395	The amphiphilic nature	374:395	The amphiphilic nature of the polysaccharide derivative along with its thermotropic behavior and ionotropic crosslinking features	374:502	The amphiphilic nature of the polysaccharide derivative along with its thermotropic behavior and ionotropic crosslinking features make possible to produce injectable bone mimetic scaffolds that can be used to release viable cells and osteoinductive biomolecules.
34755459	0	53	theme	Gum	51:53	arg1	Derivative					55:64	Alkyl Functionalized Gellan Gum Derivative	23:64	Alkyl Functionalized Gellan Gum Derivative	23:64	Composite Hydrogels of Alkyl Functionalized Gellan Gum Derivative and Hydroxyapatite/Tricalcium Phosphate Nanoparticles as Injectable Scaffolds for bone Regeneration.
34755459	1	54	contain	containing	247:256	arg1	hydrogels					237:245	hydrogels	237:245	hydrogels containing hydroxyapatite and tricalcium phosphate nanoparticles as injectable nanostructured scaffolds for bone regeneration	237:371	An alkyl functionalized gellan gum derivative is here used to produce hydrogels containing hydroxyapatite and tricalcium phosphate nanoparticles as injectable nanostructured scaffolds for bone regeneration.
34755459	1	54	contain	containing	247:256	arg2	nanoparticles					298:310	hydroxyapatite and tricalcium phosphate nanoparticles	258:310	hydroxyapatite and tricalcium phosphate nanoparticles as injectable nanostructured scaffolds for bone regeneration	258:371	An alkyl functionalized gellan gum derivative is here used to produce hydrogels containing hydroxyapatite and tricalcium phosphate nanoparticles as injectable nanostructured scaffolds for bone regeneration.
34755459	3	55	theme	rheological	699:709	arg1	properties					731:740	the rheological and physicochemical properties	695:740	the rheological and physicochemical properties of the injectable systems	695:766	The influence of different nanoparticles concentration on the rheological and physicochemical properties of the injectable systems is studied.
34755459	2	56	theme	polysaccharide	404:417	arg1	derivative					419:428	the polysaccharide derivative	400:428	the polysaccharide derivative	400:428	The amphiphilic nature of the polysaccharide derivative along with its thermotropic behavior and ionotropic crosslinking features make possible to produce injectable bone mimetic scaffolds that can be used to release viable cells and osteoinductive biomolecules.
34755459	5	57	theme	preosteoblast	1104:1116	arg1	cells					1118:1122	murine preosteoblast cells	1097:1122	murine preosteoblast cells	1097:1122	Preliminary cytocompatibility tests performed with murine preosteoblast cells revealed that gellan gum based hydrogels can safely encapsulate viable cells.
34755459	1	58	used	used	221:224	arg2	derivative					202:211	An alkyl functionalized gellan gum derivative	167:211	An alkyl functionalized gellan gum derivative	167:211	An alkyl functionalized gellan gum derivative is here used to produce hydrogels containing hydroxyapatite and tricalcium phosphate nanoparticles as injectable nanostructured scaffolds for bone regeneration.
34755459	3	59	theme	physicochemical	715:729	arg1	properties					731:740	the rheological and physicochemical properties	695:740	the rheological and physicochemical properties of the injectable systems	695:766	The influence of different nanoparticles concentration on the rheological and physicochemical properties of the injectable systems is studied.
34755459	4	60	theme	3D	852:853	arg1	networks					874:881	the 3D hydrated polymeric networks	848:881	the 3D hydrated polymeric networks	848:881	It is found that the presence of inorganic nanoparticles reinforces the 3D hydrated polymeric networks without influencing their injectability but improving the physicochemical properties of ionotropic crosslinked hydrogels produced with two different curing media.
34755459	5	61	theme	gum	1145:1147	arg1	hydrogels					1155:1163	gellan gum based hydrogels	1138:1163	gellan gum based hydrogels	1138:1163	Preliminary cytocompatibility tests performed with murine preosteoblast cells revealed that gellan gum based hydrogels can safely encapsulate viable cells.
34755459	1	62	theme	hydroxyapatite	258:271	arg1	nanoparticles					298:310	hydroxyapatite and tricalcium phosphate nanoparticles	258:310	hydroxyapatite and tricalcium phosphate nanoparticles as injectable nanostructured scaffolds for bone regeneration	258:371	An alkyl functionalized gellan gum derivative is here used to produce hydrogels containing hydroxyapatite and tricalcium phosphate nanoparticles as injectable nanostructured scaffolds for bone regeneration.
34755459	3	63	theme	injectable	749:758	arg1	systems					760:766	the injectable systems	745:766	the injectable systems	745:766	The influence of different nanoparticles concentration on the rheological and physicochemical properties of the injectable systems is studied.
34755459	6	64	theme	obtained	1332:1339	arg1	systems					1352:1358	the obtained injectable systems	1328:1358	the obtained injectable systems	1328:1358	Loading and release experiments for dexamethasone and stromal cell-derived factor-1 demonstrate the drug delivery features of the obtained injectable systems.
34755459	6	65	theme	injectable	1341:1350	arg1	systems					1352:1358	the obtained injectable systems	1328:1358	the obtained injectable systems	1328:1358	Loading and release experiments for dexamethasone and stromal cell-derived factor-1 demonstrate the drug delivery features of the obtained injectable systems.
34755459	0	66	theme	Hydroxyapatite/Tricalcium	70:94	arg1	Phosphate					96:104	Hydroxyapatite/Tricalcium Phosphate	70:104	Hydroxyapatite/Tricalcium Phosphate	70:104	Composite Hydrogels of Alkyl Functionalized Gellan Gum Derivative and Hydroxyapatite/Tricalcium Phosphate Nanoparticles as Injectable Scaffolds for bone Regeneration.
34755459	1	67	theme	alkyl	170:174	arg1	gum					198:200	An alkyl functionalized gellan gum	167:200	An alkyl functionalized gellan gum derivative	167:211	An alkyl functionalized gellan gum derivative is here used to produce hydrogels containing hydroxyapatite and tricalcium phosphate nanoparticles as injectable nanostructured scaffolds for bone regeneration.
34755459	4	68	link	crosslinked	982:992	arg1	hydrogels					994:1002	ionotropic crosslinked hydrogels	971:1002	ionotropic crosslinked hydrogels produced with two different curing media	971:1043	It is found that the presence of inorganic nanoparticles reinforces the 3D hydrated polymeric networks without influencing their injectability but improving the physicochemical properties of ionotropic crosslinked hydrogels produced with two different curing media.
34755459	1	69	theme	tricalcium	277:286	arg1	phosphate					288:296	tricalcium phosphate	277:296	tricalcium phosphate	277:296	An alkyl functionalized gellan gum derivative is here used to produce hydrogels containing hydroxyapatite and tricalcium phosphate nanoparticles as injectable nanostructured scaffolds for bone regeneration.
34755459	5	70	theme	viable	1188:1193	arg1	cells					1195:1199	viable cells	1188:1199	viable cells	1188:1199	Preliminary cytocompatibility tests performed with murine preosteoblast cells revealed that gellan gum based hydrogels can safely encapsulate viable cells.
34755459	4	71	theme	physicochemical	941:955	arg1	properties					957:966	the physicochemical properties	937:966	the physicochemical properties of ionotropic crosslinked hydrogels produced with two different curing media	937:1043	It is found that the presence of inorganic nanoparticles reinforces the 3D hydrated polymeric networks without influencing their injectability but improving the physicochemical properties of ionotropic crosslinked hydrogels produced with two different curing media.
34755459	1	72	theme	functionalized	176:189	arg1	gum					198:200	An alkyl functionalized gellan gum	167:200	An alkyl functionalized gellan gum derivative	167:211	An alkyl functionalized gellan gum derivative is here used to produce hydrogels containing hydroxyapatite and tricalcium phosphate nanoparticles as injectable nanostructured scaffolds for bone regeneration.
34755459	1	73	theme	phosphate	288:296	arg1	nanoparticles					298:310	hydroxyapatite and tricalcium phosphate nanoparticles	258:310	hydroxyapatite and tricalcium phosphate nanoparticles as injectable nanostructured scaffolds for bone regeneration	258:371	An alkyl functionalized gellan gum derivative is here used to produce hydrogels containing hydroxyapatite and tricalcium phosphate nanoparticles as injectable nanostructured scaffolds for bone regeneration.
34464793	6	0	theme	colloidal	1372:1380	arg1	stability					1382:1390	stronger colloidal stability	1363:1390	stronger colloidal stability	1363:1390	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	6	1	with	concentrations	1091:1104	arg1	strength					1187:1194	the NaCl ionic strength	1172:1194	the NaCl ionic strength	1172:1194	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	6	2	theme	colloids	1419:1426	arg1	stability					1382:1390	stronger colloidal stability	1363:1390	stronger colloidal stability	1363:1390	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	6	2	theme	colloids	1419:1426	arg1	mobility					1396:1403	mobility	1396:1403	mobility	1396:1403	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	1	3	theme	colloids	156:163	arg1	mechanism					135:143	the transport mechanism	121:143	the transport mechanism of biochar colloids in porous medium	121:180	Numerous studies have explored the transport mechanism of biochar colloids in porous medium.
34464793	0	4	theme	biochar	72:78	arg1	colloids					80:87	lignin- and cellulose-rich biochar colloids	45:87	lignin- and cellulose-rich biochar colloids	45:87	Carbon stability and mobility of ball milled lignin- and cellulose-rich biochar colloids.
34464793	6	5	theme	 < PW300	1273:1280	arg1	order					1244:1248	the increase order	1231:1248	the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids	1231:1460	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	6	6	theme	increase	1235:1242	arg1	order					1244:1248	the increase order	1231:1248	the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids	1231:1460	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	6	7	theme	critical	1070:1077	arg1	CCC					1107:1109	CCC	1107:1109	CCC	1107:1109	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	6	7	theme	critical	1070:1077	arg1	concentrations					1091:1104	the critical coagulation concentrations	1066:1104	the critical coagulation concentrations (CCC)	1066:1110	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	5	8	dep	electronegativity	908:924	arg1	-41.6-28.3 mV					940:952	-41.6-28.3 mV	940:952	-41.6-28.3 mV	940:952	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	5	8	dep	electronegativity	908:924	arg1	-44.9 mV					927:934	-44.9 mV	927:934	-44.9 mV	927:934	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	3	9	theme	milled	361:366	arg1	colloids					376:383	four ball milled biochar colloids	351:383	four ball milled biochar colloids pyrolyzed from lignin-rich pinewoods and cellulose-rich corn stalks under 300 °C and 500 °C (termed as PW300, PW500, CS300, CS500)	351:514	This study prepared four ball milled biochar colloids pyrolyzed from lignin-rich pinewoods and cellulose-rich corn stalks under 300 °C and 500 °C (termed as PW300, PW500, CS300, CS500) and analyzed their differences in the chemical stability and transport behaviors.
34464793	3	10	dep	300 °C	459:464	arg1	termed					478:483	termed	478:483	termed as PW300, PW500, CS300, CS500	478:513	This study prepared four ball milled biochar colloids pyrolyzed from lignin-rich pinewoods and cellulose-rich corn stalks under 300 °C and 500 °C (termed as PW300, PW500, CS300, CS500) and analyzed their differences in the chemical stability and transport behaviors.
34464793	4	11	theme	elemental	773:781	arg1	composition					783:793	the elemental composition	769:793	the elemental composition	769:793	The results indicated that high contents of lignin in biomass and pyrolytic temperature could enhance the compact aromatic structures of biochar colloids characterized by the elemental composition, FTIR, 13C NMR and XRD analyses.
34464793	3	12	from	differences	535:545	arg1	behaviors					587:595	transport behaviors	577:595	transport behaviors	577:595	This study prepared four ball milled biochar colloids pyrolyzed from lignin-rich pinewoods and cellulose-rich corn stalks under 300 °C and 500 °C (termed as PW300, PW500, CS300, CS500) and analyzed their differences in the chemical stability and transport behaviors.
34464793	3	12	from	differences	535:545	arg1	stability					563:571	the chemical stability	550:571	the chemical stability	550:571	This study prepared four ball milled biochar colloids pyrolyzed from lignin-rich pinewoods and cellulose-rich corn stalks under 300 °C and 500 °C (termed as PW300, PW500, CS300, CS500) and analyzed their differences in the chemical stability and transport behaviors.
34464793	3	13	theme	biochar	368:374	arg1	colloids					376:383	four ball milled biochar colloids	351:383	four ball milled biochar colloids pyrolyzed from lignin-rich pinewoods and cellulose-rich corn stalks under 300 °C and 500 °C (termed as PW300, PW500, CS300, CS500)	351:514	This study prepared four ball milled biochar colloids pyrolyzed from lignin-rich pinewoods and cellulose-rich corn stalks under 300 °C and 500 °C (termed as PW300, PW500, CS300, CS500) and analyzed their differences in the chemical stability and transport behaviors.
34464793	6	14	theme	maximum	1120:1126	arg1	concentration					1146:1158	the maximum relative effluent concentration	1116:1158	the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM	1116:1202	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	6	14	theme	maximum	1120:1126	arg1	C/C0					1161:1164	C/C0	1161:1164	C/C0	1161:1164	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	5	15	theme	C	892:892	arg1	loss					894:897	least C loss	886:897	least C loss of 13%	886:904	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	6	16	theme	biochar	1411:1417	arg1	colloids					1419:1426	PW biochar colloids	1408:1426	PW biochar colloids	1408:1426	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	7	17	theme	%	1526:1526	arg1	%					1530:1530	about 7.3%-36%	1517:1530	about 7.3%-36%	1517:1530	In addition, the C/C0 for CS300, PW300 and CS500 were about 7.3%-36% lower than that for PW500 with the NaCl ionic strength increasing to 50 mM indicated the notable superiority in the mobility of PW500.
34464793	4	18	theme	colloids	743:750	arg1	structures					721:730	the compact aromatic structures	700:730	the compact aromatic structures of biochar colloids characterized by the elemental composition, FTIR, 13C NMR and XRD analyses	700:825	The results indicated that high contents of lignin in biomass and pyrolytic temperature could enhance the compact aromatic structures of biochar colloids characterized by the elemental composition, FTIR, 13C NMR and XRD analyses.
34464793	7	19	theme	ionic	1572:1576	arg1	strength					1578:1585	the NaCl ionic strength	1563:1585	the NaCl ionic strength	1563:1585	In addition, the C/C0 for CS300, PW300 and CS500 were about 7.3%-36% lower than that for PW500 with the NaCl ionic strength increasing to 50 mM indicated the notable superiority in the mobility of PW500.
34464793	5	20	theme	chemical	864:871	arg1	diameter					981:988	smallest hydrodynamic diameter	959:988	smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm)	959:1016	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	5	20	theme	chemical	864:871	arg1	electronegativity					908:924	electronegativity	908:924	electronegativity (-44.9 mV vs. -41.6-28.3 mV)	908:953	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	5	20	theme	chemical	864:871	arg1	stabilities					873:883	the strongest chemical stabilities	850:883	the strongest chemical stabilities (least C loss of 13%)	850:905	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	1	21	theme	porous	168:173	arg1	medium					175:180	porous medium	168:180	porous medium	168:180	Numerous studies have explored the transport mechanism of biochar colloids in porous medium.
34464793	3	22	theme	ball	356:359	arg1	colloids					376:383	four ball milled biochar colloids	351:383	four ball milled biochar colloids pyrolyzed from lignin-rich pinewoods and cellulose-rich corn stalks under 300 °C and 500 °C (termed as PW300, PW500, CS300, CS500)	351:514	This study prepared four ball milled biochar colloids pyrolyzed from lignin-rich pinewoods and cellulose-rich corn stalks under 300 °C and 500 °C (termed as PW300, PW500, CS300, CS500) and analyzed their differences in the chemical stability and transport behaviors.
34464793	3	23	theme	cellulose-rich	426:439	arg1	stalks					446:451	cellulose-rich corn stalks	426:451	cellulose-rich corn stalks	426:451	This study prepared four ball milled biochar colloids pyrolyzed from lignin-rich pinewoods and cellulose-rich corn stalks under 300 °C and 500 °C (termed as PW300, PW500, CS300, CS500) and analyzed their differences in the chemical stability and transport behaviors.
34464793	3	24	theme	lignin-rich	400:410	arg1	pinewoods					412:420	lignin-rich pinewoods	400:420	lignin-rich pinewoods	400:420	This study prepared four ball milled biochar colloids pyrolyzed from lignin-rich pinewoods and cellulose-rich corn stalks under 300 °C and 500 °C (termed as PW300, PW500, CS300, CS500) and analyzed their differences in the chemical stability and transport behaviors.
34464793	5	25	theme	strongest	854:862	arg1	diameter					981:988	smallest hydrodynamic diameter	959:988	smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm)	959:1016	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	5	25	theme	strongest	854:862	arg1	electronegativity					908:924	electronegativity	908:924	electronegativity (-44.9 mV vs. -41.6-28.3 mV)	908:953	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	5	25	theme	strongest	854:862	arg1	stabilities					873:883	the strongest chemical stabilities	850:883	the strongest chemical stabilities (least C loss of 13%)	850:905	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	6	26	theme	relative	1128:1135	arg1	concentration					1146:1158	the maximum relative effluent concentration	1116:1158	the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM	1116:1202	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	6	26	theme	relative	1128:1135	arg1	C/C0					1161:1164	C/C0	1161:1164	C/C0	1161:1164	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	1	27	from	mechanism	135:143	arg1	medium					175:180	porous medium	168:180	porous medium	168:180	Numerous studies have explored the transport mechanism of biochar colloids in porous medium.
34464793	3	28	theme	chemical	554:561	arg1	stability					563:571	the chemical stability	550:571	the chemical stability	550:571	This study prepared four ball milled biochar colloids pyrolyzed from lignin-rich pinewoods and cellulose-rich corn stalks under 300 °C and 500 °C (termed as PW300, PW500, CS300, CS500) and analyzed their differences in the chemical stability and transport behaviors.
34464793	4	29	theme	pyrolytic	664:672	arg1	temperature					674:684	pyrolytic temperature	664:684	pyrolytic temperature	664:684	The results indicated that high contents of lignin in biomass and pyrolytic temperature could enhance the compact aromatic structures of biochar colloids characterized by the elemental composition, FTIR, 13C NMR and XRD analyses.
34464793	0	30	theme	Carbon	0:5	arg1	stability					7:15	Carbon stability	0:15	Carbon stability	0:15	Carbon stability and mobility of ball milled lignin- and cellulose-rich biochar colloids.
34464793	6	31	with	concentration	1146:1158	arg1	strength					1187:1194	the NaCl ionic strength	1172:1194	the NaCl ionic strength	1172:1194	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	7	32	theme	NaCl	1567:1570	arg1	strength					1578:1585	the NaCl ionic strength	1563:1585	the NaCl ionic strength	1563:1585	In addition, the C/C0 for CS300, PW300 and CS500 were about 7.3%-36% lower than that for PW500 with the NaCl ionic strength increasing to 50 mM indicated the notable superiority in the mobility of PW500.
34464793	1	33	theme	Numerous	90:97	arg1	studies					99:105	Numerous studies	90:105	Numerous studies	90:105	Numerous studies have explored the transport mechanism of biochar colloids in porous medium.
34464793	6	34	dep	 < PW300	1273:1280	arg1	%					1343:1343	563.1 mM, 95%	1331:1343	563.1 mM, 95%	1331:1343	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	6	34	dep	 < PW300	1273:1280	arg1	 < PW500					1321:1328	 < PW500	1321:1328	 < PW500	1321:1328	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	6	34	dep	 < PW300	1273:1280	arg1	%					1319:1319	363.9 mM, 89%	1307:1319	363.9 mM, 89%	1307:1319	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	5	35	with	PW500	839:843	arg1	diameter					981:988	smallest hydrodynamic diameter	959:988	smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm)	959:1016	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	5	35	with	PW500	839:843	arg1	electronegativity					908:924	electronegativity	908:924	electronegativity (-44.9 mV vs. -41.6-28.3 mV)	908:953	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	5	35	with	PW500	839:843	arg1	stabilities					873:883	the strongest chemical stabilities	850:883	the strongest chemical stabilities (least C loss of 13%)	850:905	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	2	36	theme	feedstock	206:214	arg1	biopolymer					216:225	feedstock biopolymer compositions and pyrolytic temperature	206:264	biopolymer	216:225	However, the effect of feedstock biopolymer compositions and pyrolytic temperature on carbon stability and mobility of biochar colloids is limited.
34464793	2	37	theme	colloids	310:317	arg1	mobility					290:297	mobility	290:297	mobility	290:297	However, the effect of feedstock biopolymer compositions and pyrolytic temperature on carbon stability and mobility of biochar colloids is limited.
34464793	2	37	theme	colloids	310:317	arg1	stability					276:284	carbon stability	269:284	carbon stability	269:284	However, the effect of feedstock biopolymer compositions and pyrolytic temperature on carbon stability and mobility of biochar colloids is limited.
34464793	6	38	theme	1 mM	1199:1202	arg1	concentration					1146:1158	the maximum relative effluent concentration	1116:1158	the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM	1116:1202	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	6	38	theme	1 mM	1199:1202	arg1	CCC					1107:1109	CCC	1107:1109	CCC	1107:1109	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	6	38	theme	1 mM	1199:1202	arg1	concentrations					1091:1104	the critical coagulation concentrations	1066:1104	the critical coagulation concentrations (CCC)	1066:1110	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	6	38	theme	1 mM	1199:1202	arg1	C/C0					1161:1164	C/C0	1161:1164	C/C0	1161:1164	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	2	39	dep	biopolymer	216:225	arg1	compositions					227:238	compositions	227:238	compositions	227:238	However, the effect of feedstock biopolymer compositions and pyrolytic temperature on carbon stability and mobility of biochar colloids is limited.
34464793	4	40	theme	biochar	735:741	arg1	colloids					743:750	biochar colloids	735:750	biochar colloids characterized by the elemental composition, FTIR, 13C NMR and XRD analyses	735:825	The results indicated that high contents of lignin in biomass and pyrolytic temperature could enhance the compact aromatic structures of biochar colloids characterized by the elemental composition, FTIR, 13C NMR and XRD analyses.
34464793	2	41	theme	biochar	302:308	arg1	colloids					310:317	biochar colloids	302:317	biochar colloids	302:317	However, the effect of feedstock biopolymer compositions and pyrolytic temperature on carbon stability and mobility of biochar colloids is limited.
34464793	4	42	from	contents	630:637	arg1	biomass					652:658	biomass	652:658	biomass	652:658	The results indicated that high contents of lignin in biomass and pyrolytic temperature could enhance the compact aromatic structures of biochar colloids characterized by the elemental composition, FTIR, 13C NMR and XRD analyses.
34464793	4	42	from	contents	630:637	arg1	temperature					674:684	pyrolytic temperature	664:684	pyrolytic temperature	664:684	The results indicated that high contents of lignin in biomass and pyrolytic temperature could enhance the compact aromatic structures of biochar colloids characterized by the elemental composition, FTIR, 13C NMR and XRD analyses.
34464793	4	43	theme	high	625:628	arg1	contents					630:637	high contents	625:637	high contents of lignin in biomass and pyrolytic temperature	625:684	The results indicated that high contents of lignin in biomass and pyrolytic temperature could enhance the compact aromatic structures of biochar colloids characterized by the elemental composition, FTIR, 13C NMR and XRD analyses.
34464793	0	44	theme	ball	33:36	arg1	mobility					21:28	mobility	21:28	mobility	21:28	Carbon stability and mobility of ball milled lignin- and cellulose-rich biochar colloids.
34464793	0	44	theme	ball	33:36	arg1	stability					7:15	Carbon stability	0:15	Carbon stability	0:15	Carbon stability and mobility of ball milled lignin- and cellulose-rich biochar colloids.
34464793	6	45	theme	PW	1408:1409	arg1	colloids					1419:1426	PW biochar colloids	1408:1426	PW biochar colloids	1408:1426	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	4	46	theme	XRD	814:816	arg1	analyses					818:825	XRD analyses	814:825	XRD analyses	814:825	The results indicated that high contents of lignin in biomass and pyrolytic temperature could enhance the compact aromatic structures of biochar colloids characterized by the elemental composition, FTIR, 13C NMR and XRD analyses.
34464793	6	47	theme	stronger	1363:1370	arg1	stability					1382:1390	stronger colloidal stability	1363:1390	stronger colloidal stability	1363:1390	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	4	48	theme	lignin	642:647	arg1	contents					630:637	high contents	625:637	high contents of lignin in biomass and pyrolytic temperature	625:684	The results indicated that high contents of lignin in biomass and pyrolytic temperature could enhance the compact aromatic structures of biochar colloids characterized by the elemental composition, FTIR, 13C NMR and XRD analyses.
34464793	3	49	theme	corn	441:444	arg1	stalks					446:451	cellulose-rich corn stalks	426:451	cellulose-rich corn stalks	426:451	This study prepared four ball milled biochar colloids pyrolyzed from lignin-rich pinewoods and cellulose-rich corn stalks under 300 °C and 500 °C (termed as PW300, PW500, CS300, CS500) and analyzed their differences in the chemical stability and transport behaviors.
34464793	8	50	theme	biochar	1803:1809	arg1	colloids					1811:1818	biochar colloids	1803:1818	biochar colloids	1803:1818	These findings can provide new insights toward understanding the transformation and migration, and evaluating the environmental risk of biochar colloids.
34464793	5	51	dep	stabilities	873:883	arg1	loss					894:897	least C loss	886:897	least C loss of 13%	886:904	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	7	52	theme	PW500	1660:1664	arg1	mobility					1648:1655	the mobility	1644:1655	the mobility of PW500	1644:1664	In addition, the C/C0 for CS300, PW300 and CS500 were about 7.3%-36% lower than that for PW500 with the NaCl ionic strength increasing to 50 mM indicated the notable superiority in the mobility of PW500.
34464793	2	53	from	effect	196:201	arg1	mobility					290:297	mobility	290:297	mobility	290:297	However, the effect of feedstock biopolymer compositions and pyrolytic temperature on carbon stability and mobility of biochar colloids is limited.
34464793	2	53	from	effect	196:201	arg1	stability					276:284	carbon stability	269:284	carbon stability	269:284	However, the effect of feedstock biopolymer compositions and pyrolytic temperature on carbon stability and mobility of biochar colloids is limited.
34464793	0	54	theme	lignin-	45:51	arg1	colloids					80:87	lignin- and cellulose-rich biochar colloids	45:87	lignin- and cellulose-rich biochar colloids	45:87	Carbon stability and mobility of ball milled lignin- and cellulose-rich biochar colloids.
34464793	2	55	theme	biopolymer	216:225	arg1	effect					196:201	the effect	192:201	the effect of feedstock biopolymer compositions and pyrolytic temperature on carbon stability and mobility of biochar colloids	192:317	However, the effect of feedstock biopolymer compositions and pyrolytic temperature on carbon stability and mobility of biochar colloids is limited.
34464793	4	56	theme	aromatic	712:719	arg1	structures					721:730	the compact aromatic structures	700:730	the compact aromatic structures of biochar colloids characterized by the elemental composition, FTIR, 13C NMR and XRD analyses	700:825	The results indicated that high contents of lignin in biomass and pyrolytic temperature could enhance the compact aromatic structures of biochar colloids characterized by the elemental composition, FTIR, 13C NMR and XRD analyses.
34464793	3	57	theme	transport	577:585	arg1	behaviors					587:595	transport behaviors	577:595	transport behaviors	577:595	This study prepared four ball milled biochar colloids pyrolyzed from lignin-rich pinewoods and cellulose-rich corn stalks under 300 °C and 500 °C (termed as PW300, PW500, CS300, CS500) and analyzed their differences in the chemical stability and transport behaviors.
34464793	6	58	theme	biochar	1445:1451	arg1	colloids					1453:1460	CS biochar colloids	1442:1460	CS biochar colloids	1442:1460	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	4	59	theme	compact	704:710	arg1	structures					721:730	the compact aromatic structures	700:730	the compact aromatic structures of biochar colloids characterized by the elemental composition, FTIR, 13C NMR and XRD analyses	700:825	The results indicated that high contents of lignin in biomass and pyrolytic temperature could enhance the compact aromatic structures of biochar colloids characterized by the elemental composition, FTIR, 13C NMR and XRD analyses.
34464793	6	60	theme	ionic	1181:1185	arg1	strength					1187:1194	the NaCl ionic strength	1172:1194	the NaCl ionic strength	1172:1194	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	4	61	theme	13C	802:804	arg1	NMR					806:808	13C NMR	802:808	13C NMR	802:808	The results indicated that high contents of lignin in biomass and pyrolytic temperature could enhance the compact aromatic structures of biochar colloids characterized by the elemental composition, FTIR, 13C NMR and XRD analyses.
34464793	5	62	theme	smallest	959:966	arg1	diameter					981:988	smallest hydrodynamic diameter	959:988	smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm)	959:1016	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	5	62	theme	smallest	959:966	arg1	stabilities					873:883	the strongest chemical stabilities	850:883	the strongest chemical stabilities (least C loss of 13%)	850:905	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	2	63	theme	carbon	269:274	arg1	stability					276:284	carbon stability	269:284	carbon stability	269:284	However, the effect of feedstock biopolymer compositions and pyrolytic temperature on carbon stability and mobility of biochar colloids is limited.
34464793	6	64	theme	CS	1442:1443	arg1	colloids					1453:1460	CS biochar colloids	1442:1460	CS biochar colloids	1442:1460	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	1	65	theme	transport	125:133	arg1	mechanism					135:143	the transport mechanism	121:143	the transport mechanism of biochar colloids in porous medium	121:180	Numerous studies have explored the transport mechanism of biochar colloids in porous medium.
34464793	6	66	theme	effluent	1137:1144	arg1	concentration					1146:1158	the maximum relative effluent concentration	1116:1158	the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM	1116:1202	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	6	66	theme	effluent	1137:1144	arg1	C/C0					1161:1164	C/C0	1161:1164	C/C0	1161:1164	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	6	67	theme	NaCl	1176:1179	arg1	strength					1187:1194	the NaCl ionic strength	1172:1194	the NaCl ionic strength	1172:1194	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	5	68	theme	hydrodynamic	968:979	arg1	diameter					981:988	smallest hydrodynamic diameter	959:988	smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm)	959:1016	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	5	68	theme	hydrodynamic	968:979	arg1	stabilities					873:883	the strongest chemical stabilities	850:883	the strongest chemical stabilities (least C loss of 13%)	850:905	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	5	69	theme	least	886:890	arg1	loss					894:897	least C loss	886:897	least C loss of 13%	886:904	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	5	70	theme	%	904:904	arg1	loss					894:897	least C loss	886:897	least C loss of 13%	886:904	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	0	71	theme	cellulose-rich	57:70	arg1	colloids					80:87	lignin- and cellulose-rich biochar colloids	45:87	lignin- and cellulose-rich biochar colloids	45:87	Carbon stability and mobility of ball milled lignin- and cellulose-rich biochar colloids.
34464793	5	72	theme	ball	1037:1040	arg1	milling					1042:1048	ball milling	1037:1048	ball milling	1037:1048	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	2	73	theme	temperature	254:264	arg1	effect					196:201	the effect	192:201	the effect of feedstock biopolymer compositions and pyrolytic temperature on carbon stability and mobility of biochar colloids	192:317	However, the effect of feedstock biopolymer compositions and pyrolytic temperature on carbon stability and mobility of biochar colloids is limited.
34464793	5	74	dep	diameter	981:988	arg1	622-997.2 nm					1004:1015	622-997.2 nm	1004:1015	622-997.2 nm	1004:1015	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	5	74	dep	diameter	981:988	arg1	608.7 nm					991:998	608.7 nm	991:998	608.7 nm	991:998	Therefore, PW500 with the strongest chemical stabilities (least C loss of 13%), electronegativity (-44.9 mV vs. -41.6-28.3 mV) and smallest hydrodynamic diameter (608.7 nm vs. 622-997.2 nm) was obtained under ball milling.
34464793	6	75	theme	coagulation	1079:1089	arg1	CCC					1107:1109	CCC	1107:1109	CCC	1107:1109	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	6	75	theme	coagulation	1079:1089	arg1	concentrations					1091:1104	the critical coagulation concentrations	1066:1104	the critical coagulation concentrations (CCC)	1066:1110	Moreover, both the critical coagulation concentrations (CCC) and the maximum relative effluent concentration (C/C0) with the NaCl ionic strength of 1 mM were demonstrated to be in the increase order of CS300 (76.1 mM, 70%) < PW300 (183.1 mM, 78%) < CS500 (363.9 mM, 89%) < PW500 (563.1 mM, 95%), which suggested stronger colloidal stability and mobility of PW biochar colloids than those of CS biochar colloids.
34464793	7	76	from	superiority	1629:1639	arg1	mobility					1648:1655	the mobility	1644:1655	the mobility of PW500	1644:1664	In addition, the C/C0 for CS300, PW300 and CS500 were about 7.3%-36% lower than that for PW500 with the NaCl ionic strength increasing to 50 mM indicated the notable superiority in the mobility of PW500.
34464793	8	77	theme	environmental	1781:1793	arg1	risk					1795:1798	the environmental risk	1777:1798	the environmental risk of biochar colloids	1777:1818	These findings can provide new insights toward understanding the transformation and migration, and evaluating the environmental risk of biochar colloids.
34464793	8	78	theme	new	1694:1696	arg1	insights					1698:1705	new insights	1694:1705	new insights toward understanding the transformation and migration, and evaluating the environmental risk of biochar colloids	1694:1818	These findings can provide new insights toward understanding the transformation and migration, and evaluating the environmental risk of biochar colloids.
34464793	2	79	theme	pyrolytic	244:252	arg1	temperature					254:264	pyrolytic temperature	244:264	pyrolytic temperature	244:264	However, the effect of feedstock biopolymer compositions and pyrolytic temperature on carbon stability and mobility of biochar colloids is limited.
34464793	7	80	theme	notable	1621:1627	arg1	superiority					1629:1639	the notable superiority	1617:1639	the notable superiority in the mobility of PW500	1617:1664	In addition, the C/C0 for CS300, PW300 and CS500 were about 7.3%-36% lower than that for PW500 with the NaCl ionic strength increasing to 50 mM indicated the notable superiority in the mobility of PW500.
34464793	1	81	theme	biochar	148:154	arg1	colloids					156:163	biochar colloids	148:163	biochar colloids	148:163	Numerous studies have explored the transport mechanism of biochar colloids in porous medium.
34464793	8	82	theme	colloids	1811:1818	arg1	risk					1795:1798	the environmental risk	1777:1798	the environmental risk of biochar colloids	1777:1818	These findings can provide new insights toward understanding the transformation and migration, and evaluating the environmental risk of biochar colloids.
34212172	0	0	theme	mechanical	108:117	arg1	properties					119:128	high mechanical properties	103:128	high mechanical properties	103:128	Bioprocess-inspired synthesis of multilayered chitosan/CaCO3 composites with nacre-like structures and high mechanical properties.
34212172	4	1	theme	manufacturing	759:771	arg1	method					788:793	a microscale additive manufacturing mineralization method	737:793	a microscale additive manufacturing mineralization method	737:793	Inspired by the nacre formation process, a microscale additive manufacturing mineralization method is proposed for achieving a multilayered organic-inorganic layered structure.
34212172	4	2	theme	layered	854:860	arg1	structure					862:870	a multilayered organic-inorganic layered structure	821:870	a multilayered organic-inorganic layered structure	821:870	Inspired by the nacre formation process, a microscale additive manufacturing mineralization method is proposed for achieving a multilayered organic-inorganic layered structure.
34212172	8	3	theme	biological	1476:1485	arg1	counterparts					1487:1498	their biological counterparts	1470:1498	their biological counterparts	1470:1498	Impressively, the hardness (2.35 ± 0.03 GPa) and Young's modulus (58.1 ± 0.5 GPa) of the as-prepared (CS/CaCO3) composites are comparable to those of their biological counterparts.
34212172	3	4	theme	calcium	555:561	arg1	carbonate					563:571	crystalline calcium carbonate	543:571	crystalline calcium carbonate lamellae	543:580	Nacre consists of an ordered multilayer structure of crystalline calcium carbonate lamellae separated by organic layers exhibiting mechanical toughness, which transcends that of its constituent components.
34212172	0	5	theme	high	103:106	arg1	properties					119:128	high mechanical properties	103:128	high mechanical properties	103:128	Bioprocess-inspired synthesis of multilayered chitosan/CaCO3 composites with nacre-like structures and high mechanical properties.
34212172	1	6	located	found	167:171	arg1	systems					187:193	biological systems	176:193	biological systems	176:193	The formation of natural structures found in biological systems is wonderful and can be completed at ambient temperatures in contrast to artificial technologies wherein harsh conditions are common prerequisites.
34212172	1	6	located	found	167:171	arg2	structures					156:165	natural structures	148:165	natural structures found in biological systems	148:193	The formation of natural structures found in biological systems is wonderful and can be completed at ambient temperatures in contrast to artificial technologies wherein harsh conditions are common prerequisites.
34212172	9	7	with	materials	1621:1629	arg1	performance					1641:1651	good performance	1636:1651	good performance	1636:1651	This study provides a rational bioprocess-inspired room-temperature mineralization method to develop advanced composite materials with good performance.
34212172	2	8	theme	manufacturing	390:402	arg1	"					403:403	"bioprocess inspired manufacturing"	369:403	"bioprocess inspired manufacturing"	369:403	A new research direction, "bioprocess inspired manufacturing", is proposed for fabricating advanced materials with novel structures and functions.
34212172	2	8	theme	manufacturing	390:402	arg1	direction					358:366	A new research direction	343:366	A new research direction	343:366	A new research direction, "bioprocess inspired manufacturing", is proposed for fabricating advanced materials with novel structures and functions.
34212172	9	9	theme	room-temperature	1552:1567	arg1	method					1584:1589	a rational bioprocess-inspired room-temperature mineralization method	1521:1589	a rational bioprocess-inspired room-temperature mineralization method to develop advanced composite materials with good performance	1521:1651	This study provides a rational bioprocess-inspired room-temperature mineralization method to develop advanced composite materials with good performance.
34212172	8	10	theme	CS/CaCO3	1422:1429	arg1	composites					1432:1441	the as-prepared (CS/CaCO3) composites	1405:1441	the as-prepared (CS/CaCO3) composites	1405:1441	Impressively, the hardness (2.35 ± 0.03 GPa) and Young's modulus (58.1 ± 0.5 GPa) of the as-prepared (CS/CaCO3) composites are comparable to those of their biological counterparts.
34212172	9	11	theme	advanced	1602:1609	arg1	materials					1621:1629	advanced composite materials	1602:1629	advanced composite materials with good performance	1602:1651	This study provides a rational bioprocess-inspired room-temperature mineralization method to develop advanced composite materials with good performance.
34212172	4	12	theme	organic-inorganic	836:852	arg1	structure					862:870	a multilayered organic-inorganic layered structure	821:870	a multilayered organic-inorganic layered structure	821:870	Inspired by the nacre formation process, a microscale additive manufacturing mineralization method is proposed for achieving a multilayered organic-inorganic layered structure.
34212172	1	13	theme	harsh	300:304	arg1	prerequisites					328:340	common prerequisites	321:340	common prerequisites	321:340	The formation of natural structures found in biological systems is wonderful and can be completed at ambient temperatures in contrast to artificial technologies wherein harsh conditions are common prerequisites.
34212172	1	13	theme	harsh	300:304	arg1	conditions					306:315	harsh conditions	300:315	harsh conditions	300:315	The formation of natural structures found in biological systems is wonderful and can be completed at ambient temperatures in contrast to artificial technologies wherein harsh conditions are common prerequisites.
34212172	5	14	theme	ions	1020:1023	arg1	control					999:1005	the coordinated control	983:1005	the coordinated control of magnesium ions (Mg2+) and polyacrylic acid (PAA)	983:1057	In this work, layered calcite was synthesized on the surface of chitosan (CS) films at room temperature under the coordinated control of magnesium ions (Mg2+) and polyacrylic acid (PAA).
34212172	9	15	theme	good	1636:1639	arg1	performance					1641:1651	good performance	1636:1651	good performance	1636:1651	This study provides a rational bioprocess-inspired room-temperature mineralization method to develop advanced composite materials with good performance.
34212172	5	16	theme	magnesium	1010:1018	arg1	Mg2+					1026:1029	Mg2+	1026:1029	Mg2+	1026:1029	In this work, layered calcite was synthesized on the surface of chitosan (CS) films at room temperature under the coordinated control of magnesium ions (Mg2+) and polyacrylic acid (PAA).
34212172	5	16	theme	magnesium	1010:1018	arg1	ions					1020:1023	magnesium ions	1010:1023	magnesium ions (Mg2+)	1010:1030	In this work, layered calcite was synthesized on the surface of chitosan (CS) films at room temperature under the coordinated control of magnesium ions (Mg2+) and polyacrylic acid (PAA).
34212172	5	17	theme	polyacrylic	1036:1046	arg1	PAA					1054:1056	PAA	1054:1056	PAA	1054:1056	In this work, layered calcite was synthesized on the surface of chitosan (CS) films at room temperature under the coordinated control of magnesium ions (Mg2+) and polyacrylic acid (PAA).
34212172	5	17	theme	polyacrylic	1036:1046	arg1	acid					1048:1051	polyacrylic acid	1036:1051	polyacrylic acid (PAA)	1036:1057	In this work, layered calcite was synthesized on the surface of chitosan (CS) films at room temperature under the coordinated control of magnesium ions (Mg2+) and polyacrylic acid (PAA).
34212172	5	18	theme	chitosan	937:944	arg1	films					951:955	chitosan (CS) films	937:955	chitosan (CS) films	937:955	In this work, layered calcite was synthesized on the surface of chitosan (CS) films at room temperature under the coordinated control of magnesium ions (Mg2+) and polyacrylic acid (PAA).
34212172	4	19	theme	mineralization	773:786	arg1	method					788:793	a microscale additive manufacturing mineralization method	737:793	a microscale additive manufacturing mineralization method	737:793	Inspired by the nacre formation process, a microscale additive manufacturing mineralization method is proposed for achieving a multilayered organic-inorganic layered structure.
34212172	2	20	with	materials	443:451	arg1	functions					479:487	functions	479:487	functions	479:487	A new research direction, "bioprocess inspired manufacturing", is proposed for fabricating advanced materials with novel structures and functions.
34212172	2	20	with	materials	443:451	arg1	structures					464:473	novel structures	458:473	novel structures	458:473	A new research direction, "bioprocess inspired manufacturing", is proposed for fabricating advanced materials with novel structures and functions.
34212172	3	21	theme	ordered	511:517	arg1	structure					530:538	an ordered multilayer structure	508:538	an ordered multilayer structure of crystalline calcium carbonate lamellae separated by organic layers exhibiting mechanical toughness, which transcends that of its constituent components	508:693	Nacre consists of an ordered multilayer structure of crystalline calcium carbonate lamellae separated by organic layers exhibiting mechanical toughness, which transcends that of its constituent components.
34212172	5	22	theme	acid	1048:1051	arg1	control					999:1005	the coordinated control	983:1005	the coordinated control of magnesium ions (Mg2+) and polyacrylic acid (PAA)	983:1057	In this work, layered calcite was synthesized on the surface of chitosan (CS) films at room temperature under the coordinated control of magnesium ions (Mg2+) and polyacrylic acid (PAA).
34212172	5	23	theme	films	951:955	arg1	surface					926:932	the surface	922:932	the surface of chitosan (CS) films	922:955	In this work, layered calcite was synthesized on the surface of chitosan (CS) films at room temperature under the coordinated control of magnesium ions (Mg2+) and polyacrylic acid (PAA).
34212172	2	24	theme	advanced	434:441	arg1	materials					443:451	advanced materials	434:451	advanced materials with novel structures and functions	434:487	A new research direction, "bioprocess inspired manufacturing", is proposed for fabricating advanced materials with novel structures and functions.
34212172	6	25	theme	hybrid	1189:1194	arg1	structure					1196:1204	an organic-inorganic hybrid structure	1168:1204	an organic-inorganic hybrid structure	1168:1204	The CS films and layered calcite are sequentially assembled in a layer-by-layer deposition approach to form an organic-inorganic hybrid structure.
34212172	8	26	theme	Young	1369:1373	arg1	GPa					1397:1399	58.1 ± 0.5 GPa	1386:1399	58.1 ± 0.5 GPa	1386:1399	Impressively, the hardness (2.35 ± 0.03 GPa) and Young's modulus (58.1 ± 0.5 GPa) of the as-prepared (CS/CaCO3) composites are comparable to those of their biological counterparts.
34212172	8	26	theme	Young	1369:1373	arg1	modulus					1377:1383	Young's modulus	1369:1383	Young's modulus (58.1 ± 0.5 GPa) of the as-prepared (CS/CaCO3) composites	1369:1441	Impressively, the hardness (2.35 ± 0.03 GPa) and Young's modulus (58.1 ± 0.5 GPa) of the as-prepared (CS/CaCO3) composites are comparable to those of their biological counterparts.
34212172	3	27	theme	organic	595:601	arg1	layers					603:608	organic layers	595:608	organic layers exhibiting mechanical toughness, which transcends that of its constituent components	595:693	Nacre consists of an ordered multilayer structure of crystalline calcium carbonate lamellae separated by organic layers exhibiting mechanical toughness, which transcends that of its constituent components.
34212172	6	28	theme	layer-by-layer	1125:1138	arg1	approach					1151:1158	a layer-by-layer deposition approach	1123:1158	a layer-by-layer deposition approach to form an organic-inorganic hybrid structure	1123:1204	The CS films and layered calcite are sequentially assembled in a layer-by-layer deposition approach to form an organic-inorganic hybrid structure.
34212172	9	29	theme	composite	1611:1619	arg1	materials					1621:1629	advanced composite materials	1602:1629	advanced composite materials with good performance	1602:1651	This study provides a rational bioprocess-inspired room-temperature mineralization method to develop advanced composite materials with good performance.
34212172	6	30	theme	deposition	1140:1149	arg1	approach					1151:1158	a layer-by-layer deposition approach	1123:1158	a layer-by-layer deposition approach to form an organic-inorganic hybrid structure	1123:1204	The CS films and layered calcite are sequentially assembled in a layer-by-layer deposition approach to form an organic-inorganic hybrid structure.
34212172	4	31	theme	multilayered	823:834	arg1	structure					862:870	a multilayered organic-inorganic layered structure	821:870	a multilayered organic-inorganic layered structure	821:870	Inspired by the nacre formation process, a microscale additive manufacturing mineralization method is proposed for achieving a multilayered organic-inorganic layered structure.
34212172	4	32	theme	microscale	739:748	arg1	method					788:793	a microscale additive manufacturing mineralization method	737:793	a microscale additive manufacturing mineralization method	737:793	Inspired by the nacre formation process, a microscale additive manufacturing mineralization method is proposed for achieving a multilayered organic-inorganic layered structure.
34212172	0	33	theme	Bioprocess-inspired	0:18	arg1	synthesis					20:28	Bioprocess-inspired synthesis	0:28	Bioprocess-inspired synthesis of multilayered chitosan/CaCO3 composites with nacre-like structures and high mechanical properties	0:128	Bioprocess-inspired synthesis of multilayered chitosan/CaCO3 composites with nacre-like structures and high mechanical properties.
34212172	7	34	theme	chitosan/CaCO3	1222:1235	arg1	composites					1248:1257	The nacre-like chitosan/CaCO3 (CS/CaCO3) composites	1207:1257	The nacre-like chitosan/CaCO3 (CS/CaCO3) composites	1207:1257	The nacre-like chitosan/CaCO3 (CS/CaCO3) composites exhibit high transparency and underwater superoleophobicity.
34212172	2	35	theme	research	349:356	arg1	"					403:403	"bioprocess inspired manufacturing"	369:403	"bioprocess inspired manufacturing"	369:403	A new research direction, "bioprocess inspired manufacturing", is proposed for fabricating advanced materials with novel structures and functions.
34212172	2	35	theme	research	349:356	arg1	direction					358:366	A new research direction	343:366	A new research direction	343:366	A new research direction, "bioprocess inspired manufacturing", is proposed for fabricating advanced materials with novel structures and functions.
34212172	8	36	theme	±	1353:1353	arg1	GPa					1360:1362	2.35 ± 0.03 GPa	1348:1362	2.35 ± 0.03 GPa	1348:1362	Impressively, the hardness (2.35 ± 0.03 GPa) and Young's modulus (58.1 ± 0.5 GPa) of the as-prepared (CS/CaCO3) composites are comparable to those of their biological counterparts.
34212172	8	36	theme	±	1353:1353	arg1	hardness					1338:1345	the hardness	1334:1345	the hardness (2.35 ± 0.03 GPa)	1334:1363	Impressively, the hardness (2.35 ± 0.03 GPa) and Young's modulus (58.1 ± 0.5 GPa) of the as-prepared (CS/CaCO3) composites are comparable to those of their biological counterparts.
34212172	0	37	theme	multilayered	33:44	arg1	composites					61:70	multilayered chitosan/CaCO3 composites	33:70	multilayered chitosan/CaCO3 composites	33:70	Bioprocess-inspired synthesis of multilayered chitosan/CaCO3 composites with nacre-like structures and high mechanical properties.
34212172	1	38	theme	common	321:326	arg1	prerequisites					328:340	common prerequisites	321:340	common prerequisites	321:340	The formation of natural structures found in biological systems is wonderful and can be completed at ambient temperatures in contrast to artificial technologies wherein harsh conditions are common prerequisites.
34212172	1	38	theme	common	321:326	arg1	conditions					306:315	harsh conditions	300:315	harsh conditions	300:315	The formation of natural structures found in biological systems is wonderful and can be completed at ambient temperatures in contrast to artificial technologies wherein harsh conditions are common prerequisites.
34212172	5	39	theme	coordinated	987:997	arg1	control					999:1005	the coordinated control	983:1005	the coordinated control of magnesium ions (Mg2+) and polyacrylic acid (PAA)	983:1057	In this work, layered calcite was synthesized on the surface of chitosan (CS) films at room temperature under the coordinated control of magnesium ions (Mg2+) and polyacrylic acid (PAA).
34212172	2	40	theme	new	345:347	arg1	"					403:403	"bioprocess inspired manufacturing"	369:403	"bioprocess inspired manufacturing"	369:403	A new research direction, "bioprocess inspired manufacturing", is proposed for fabricating advanced materials with novel structures and functions.
34212172	2	40	theme	new	345:347	arg1	direction					358:366	A new research direction	343:366	A new research direction	343:366	A new research direction, "bioprocess inspired manufacturing", is proposed for fabricating advanced materials with novel structures and functions.
34212172	6	41	theme	organic-inorganic	1171:1187	arg1	structure					1196:1204	an organic-inorganic hybrid structure	1168:1204	an organic-inorganic hybrid structure	1168:1204	The CS films and layered calcite are sequentially assembled in a layer-by-layer deposition approach to form an organic-inorganic hybrid structure.
34212172	3	42	theme	crystalline	543:553	arg1	carbonate					563:571	crystalline calcium carbonate	543:571	crystalline calcium carbonate lamellae	543:580	Nacre consists of an ordered multilayer structure of crystalline calcium carbonate lamellae separated by organic layers exhibiting mechanical toughness, which transcends that of its constituent components.
34212172	2	43	theme	novel	458:462	arg1	structures					464:473	novel structures	458:473	novel structures	458:473	A new research direction, "bioprocess inspired manufacturing", is proposed for fabricating advanced materials with novel structures and functions.
34212172	0	44	theme	composites	61:70	arg1	synthesis					20:28	Bioprocess-inspired synthesis	0:28	Bioprocess-inspired synthesis of multilayered chitosan/CaCO3 composites with nacre-like structures and high mechanical properties	0:128	Bioprocess-inspired synthesis of multilayered chitosan/CaCO3 composites with nacre-like structures and high mechanical properties.
34212172	2	45	theme	bioprocess	370:379	arg1	"					403:403	"bioprocess inspired manufacturing"	369:403	"bioprocess inspired manufacturing"	369:403	A new research direction, "bioprocess inspired manufacturing", is proposed for fabricating advanced materials with novel structures and functions.
34212172	2	45	theme	bioprocess	370:379	arg1	direction					358:366	A new research direction	343:366	A new research direction	343:366	A new research direction, "bioprocess inspired manufacturing", is proposed for fabricating advanced materials with novel structures and functions.
34212172	9	46	theme	mineralization	1569:1582	arg1	method					1584:1589	a rational bioprocess-inspired room-temperature mineralization method	1521:1589	a rational bioprocess-inspired room-temperature mineralization method to develop advanced composite materials with good performance	1521:1651	This study provides a rational bioprocess-inspired room-temperature mineralization method to develop advanced composite materials with good performance.
34212172	4	47	theme	formation	718:726	arg1	process					728:734	the nacre formation process	708:734	the nacre formation process	708:734	Inspired by the nacre formation process, a microscale additive manufacturing mineralization method is proposed for achieving a multilayered organic-inorganic layered structure.
34212172	1	48	theme	ambient	232:238	arg1	temperatures					240:251	ambient temperatures	232:251	ambient temperatures	232:251	The formation of natural structures found in biological systems is wonderful and can be completed at ambient temperatures in contrast to artificial technologies wherein harsh conditions are common prerequisites.
34212172	0	49	theme	chitosan/CaCO3	46:59	arg1	composites					61:70	multilayered chitosan/CaCO3 composites	33:70	multilayered chitosan/CaCO3 composites	33:70	Bioprocess-inspired synthesis of multilayered chitosan/CaCO3 composites with nacre-like structures and high mechanical properties.
34212172	7	50	theme	CS/CaCO3	1238:1245	arg1	composites					1248:1257	The nacre-like chitosan/CaCO3 (CS/CaCO3) composites	1207:1257	The nacre-like chitosan/CaCO3 (CS/CaCO3) composites	1207:1257	The nacre-like chitosan/CaCO3 (CS/CaCO3) composites exhibit high transparency and underwater superoleophobicity.
34212172	4	51	theme	nacre	712:716	arg1	process					728:734	the nacre formation process	708:734	the nacre formation process	708:734	Inspired by the nacre formation process, a microscale additive manufacturing mineralization method is proposed for achieving a multilayered organic-inorganic layered structure.
34212172	0	52	theme	nacre-like	77:86	arg1	structures					88:97	nacre-like structures	77:97	nacre-like structures	77:97	Bioprocess-inspired synthesis of multilayered chitosan/CaCO3 composites with nacre-like structures and high mechanical properties.
34212172	5	53	theme	room	960:963	arg1	temperature					965:975	room temperature	960:975	room temperature	960:975	In this work, layered calcite was synthesized on the surface of chitosan (CS) films at room temperature under the coordinated control of magnesium ions (Mg2+) and polyacrylic acid (PAA).
34212172	7	54	theme	underwater	1289:1298	arg1	superoleophobicity					1300:1317	underwater superoleophobicity	1289:1317	underwater superoleophobicity	1289:1317	The nacre-like chitosan/CaCO3 (CS/CaCO3) composites exhibit high transparency and underwater superoleophobicity.
34212172	9	55	theme	rational	1523:1530	arg1	method					1584:1589	a rational bioprocess-inspired room-temperature mineralization method	1521:1589	a rational bioprocess-inspired room-temperature mineralization method to develop advanced composite materials with good performance	1521:1651	This study provides a rational bioprocess-inspired room-temperature mineralization method to develop advanced composite materials with good performance.
34212172	8	56	theme	as-prepared	1409:1419	arg1	composites					1432:1441	the as-prepared (CS/CaCO3) composites	1405:1441	the as-prepared (CS/CaCO3) composites	1405:1441	Impressively, the hardness (2.35 ± 0.03 GPa) and Young's modulus (58.1 ± 0.5 GPa) of the as-prepared (CS/CaCO3) composites are comparable to those of their biological counterparts.
34212172	2	57	theme	inspired	381:388	arg1	"					403:403	"bioprocess inspired manufacturing"	369:403	"bioprocess inspired manufacturing"	369:403	A new research direction, "bioprocess inspired manufacturing", is proposed for fabricating advanced materials with novel structures and functions.
34212172	2	57	theme	inspired	381:388	arg1	direction					358:366	A new research direction	343:366	A new research direction	343:366	A new research direction, "bioprocess inspired manufacturing", is proposed for fabricating advanced materials with novel structures and functions.
34212172	7	58	theme	nacre-like	1211:1220	arg1	composites					1248:1257	The nacre-like chitosan/CaCO3 (CS/CaCO3) composites	1207:1257	The nacre-like chitosan/CaCO3 (CS/CaCO3) composites	1207:1257	The nacre-like chitosan/CaCO3 (CS/CaCO3) composites exhibit high transparency and underwater superoleophobicity.
34212172	8	59	theme	±	1391:1391	arg1	GPa					1397:1399	58.1 ± 0.5 GPa	1386:1399	58.1 ± 0.5 GPa	1386:1399	Impressively, the hardness (2.35 ± 0.03 GPa) and Young's modulus (58.1 ± 0.5 GPa) of the as-prepared (CS/CaCO3) composites are comparable to those of their biological counterparts.
34212172	8	59	theme	±	1391:1391	arg1	modulus					1377:1383	Young's modulus	1369:1383	Young's modulus (58.1 ± 0.5 GPa) of the as-prepared (CS/CaCO3) composites	1369:1441	Impressively, the hardness (2.35 ± 0.03 GPa) and Young's modulus (58.1 ± 0.5 GPa) of the as-prepared (CS/CaCO3) composites are comparable to those of their biological counterparts.
34212172	0	60	with	synthesis	20:28	arg1	structures					88:97	nacre-like structures	77:97	nacre-like structures	77:97	Bioprocess-inspired synthesis of multilayered chitosan/CaCO3 composites with nacre-like structures and high mechanical properties.
34212172	0	60	with	synthesis	20:28	arg1	properties					119:128	high mechanical properties	103:128	high mechanical properties	103:128	Bioprocess-inspired synthesis of multilayered chitosan/CaCO3 composites with nacre-like structures and high mechanical properties.
34212172	1	61	theme	natural	148:154	arg1	structures					156:165	natural structures	148:165	natural structures found in biological systems	148:193	The formation of natural structures found in biological systems is wonderful and can be completed at ambient temperatures in contrast to artificial technologies wherein harsh conditions are common prerequisites.
34212172	3	62	theme	carbonate	563:571	arg1	lamellae					573:580	crystalline calcium carbonate lamellae	543:580	crystalline calcium carbonate lamellae	543:580	Nacre consists of an ordered multilayer structure of crystalline calcium carbonate lamellae separated by organic layers exhibiting mechanical toughness, which transcends that of its constituent components.
34212172	8	63	theme	composites	1432:1441	arg1	comparable					1447:1456	comparable	1447:1456	comparable	1447:1456	Impressively, the hardness (2.35 ± 0.03 GPa) and Young's modulus (58.1 ± 0.5 GPa) of the as-prepared (CS/CaCO3) composites are comparable to those of their biological counterparts.
34212172	8	63	theme	composites	1432:1441	arg1	GPa					1397:1399	58.1 ± 0.5 GPa	1386:1399	58.1 ± 0.5 GPa	1386:1399	Impressively, the hardness (2.35 ± 0.03 GPa) and Young's modulus (58.1 ± 0.5 GPa) of the as-prepared (CS/CaCO3) composites are comparable to those of their biological counterparts.
34212172	8	63	theme	composites	1432:1441	arg1	modulus					1377:1383	Young's modulus	1369:1383	Young's modulus (58.1 ± 0.5 GPa) of the as-prepared (CS/CaCO3) composites	1369:1441	Impressively, the hardness (2.35 ± 0.03 GPa) and Young's modulus (58.1 ± 0.5 GPa) of the as-prepared (CS/CaCO3) composites are comparable to those of their biological counterparts.
34212172	8	63	theme	composites	1432:1441	arg1	GPa					1360:1362	2.35 ± 0.03 GPa	1348:1362	2.35 ± 0.03 GPa	1348:1362	Impressively, the hardness (2.35 ± 0.03 GPa) and Young's modulus (58.1 ± 0.5 GPa) of the as-prepared (CS/CaCO3) composites are comparable to those of their biological counterparts.
34212172	8	63	theme	composites	1432:1441	arg1	hardness					1338:1345	the hardness	1334:1345	the hardness (2.35 ± 0.03 GPa)	1334:1363	Impressively, the hardness (2.35 ± 0.03 GPa) and Young's modulus (58.1 ± 0.5 GPa) of the as-prepared (CS/CaCO3) composites are comparable to those of their biological counterparts.
34212172	1	64	theme	structures	156:165	arg1	wonderful					198:206	wonderful	198:206	wonderful	198:206	The formation of natural structures found in biological systems is wonderful and can be completed at ambient temperatures in contrast to artificial technologies wherein harsh conditions are common prerequisites.
34212172	1	64	theme	structures	156:165	arg1	formation					135:143	The formation	131:143	The formation of natural structures found in biological systems	131:193	The formation of natural structures found in biological systems is wonderful and can be completed at ambient temperatures in contrast to artificial technologies wherein harsh conditions are common prerequisites.
34212172	3	65	theme	mechanical	621:630	arg1	toughness					632:640	mechanical toughness	621:640	mechanical toughness	621:640	Nacre consists of an ordered multilayer structure of crystalline calcium carbonate lamellae separated by organic layers exhibiting mechanical toughness, which transcends that of its constituent components.
34212172	6	66	theme	layered	1077:1083	arg1	calcite					1085:1091	layered calcite	1077:1091	layered calcite	1077:1091	The CS films and layered calcite are sequentially assembled in a layer-by-layer deposition approach to form an organic-inorganic hybrid structure.
34212172	1	67	dep	technologies	279:290	arg1	wherein					292:298	wherein	292:298	artificial technologies wherein harsh conditions are common prerequisites	268:340	The formation of natural structures found in biological systems is wonderful and can be completed at ambient temperatures in contrast to artificial technologies wherein harsh conditions are common prerequisites.
34212172	4	68	theme	additive	750:757	arg1	method					788:793	a microscale additive manufacturing mineralization method	737:793	a microscale additive manufacturing mineralization method	737:793	Inspired by the nacre formation process, a microscale additive manufacturing mineralization method is proposed for achieving a multilayered organic-inorganic layered structure.
34212172	3	69	theme	multilayer	519:528	arg1	structure					530:538	an ordered multilayer structure	508:538	an ordered multilayer structure of crystalline calcium carbonate lamellae separated by organic layers exhibiting mechanical toughness, which transcends that of its constituent components	508:693	Nacre consists of an ordered multilayer structure of crystalline calcium carbonate lamellae separated by organic layers exhibiting mechanical toughness, which transcends that of its constituent components.
34212172	9	70	theme	bioprocess-inspired	1532:1550	arg1	method					1584:1589	a rational bioprocess-inspired room-temperature mineralization method	1521:1589	a rational bioprocess-inspired room-temperature mineralization method to develop advanced composite materials with good performance	1521:1651	This study provides a rational bioprocess-inspired room-temperature mineralization method to develop advanced composite materials with good performance.
34212172	1	71	dep	wherein	292:298	arg1	prerequisites					328:340	common prerequisites	321:340	common prerequisites	321:340	The formation of natural structures found in biological systems is wonderful and can be completed at ambient temperatures in contrast to artificial technologies wherein harsh conditions are common prerequisites.
34212172	1	71	dep	wherein	292:298	arg1	conditions					306:315	harsh conditions	300:315	harsh conditions	300:315	The formation of natural structures found in biological systems is wonderful and can be completed at ambient temperatures in contrast to artificial technologies wherein harsh conditions are common prerequisites.
34212172	3	72	theme	constituent	672:682	arg1	components					684:693	its constituent components	668:693	its constituent components	668:693	Nacre consists of an ordered multilayer structure of crystalline calcium carbonate lamellae separated by organic layers exhibiting mechanical toughness, which transcends that of its constituent components.
34212172	3	73	theme	lamellae	573:580	arg1	structure					530:538	an ordered multilayer structure	508:538	an ordered multilayer structure of crystalline calcium carbonate lamellae separated by organic layers exhibiting mechanical toughness, which transcends that of its constituent components	508:693	Nacre consists of an ordered multilayer structure of crystalline calcium carbonate lamellae separated by organic layers exhibiting mechanical toughness, which transcends that of its constituent components.
34212172	5	74	theme	layered	887:893	arg1	calcite					895:901	layered calcite	887:901	layered calcite	887:901	In this work, layered calcite was synthesized on the surface of chitosan (CS) films at room temperature under the coordinated control of magnesium ions (Mg2+) and polyacrylic acid (PAA).
34212172	7	75	theme	high	1267:1270	arg1	transparency					1272:1283	high transparency	1267:1283	high transparency	1267:1283	The nacre-like chitosan/CaCO3 (CS/CaCO3) composites exhibit high transparency and underwater superoleophobicity.
34212172	6	76	theme	CS	1064:1065	arg1	films					1067:1071	The CS films	1060:1071	The CS films	1060:1071	The CS films and layered calcite are sequentially assembled in a layer-by-layer deposition approach to form an organic-inorganic hybrid structure.
34212172	1	77	theme	biological	176:185	arg1	systems					187:193	biological systems	176:193	biological systems	176:193	The formation of natural structures found in biological systems is wonderful and can be completed at ambient temperatures in contrast to artificial technologies wherein harsh conditions are common prerequisites.
34212172	1	78	theme	artificial	268:277	arg1	technologies					279:290	artificial technologies wherein harsh conditions are common prerequisites	268:340	artificial technologies wherein harsh conditions are common prerequisites	268:340	The formation of natural structures found in biological systems is wonderful and can be completed at ambient temperatures in contrast to artificial technologies wherein harsh conditions are common prerequisites.
34541592	7	0	theme	wide	1156:1159	arg1	range					1161:1165	a wide range	1154:1165	a wide range of temperatures from 25 K to 1000 K	1154:1201	In addition, we calculated standard thermodynamic properties, including enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K.
34541592	4	1	theme	reaction	653:660	arg1	enthalpies					662:671	the reaction enthalpies	649:671	the reaction enthalpies of bond cleavage	649:688	Using this oligomer, the present work aims to determine if and how the reaction enthalpies of bond cleavage vary with positions within the chain.
34541592	1	2	theme	structure	169:177	arg1	methods					179:185	electronic structure methods	158:185	electronic structure methods	158:185	Computational studies on the pyrolysis of lignin using electronic structure methods have been largely limited to dimeric or trimeric models.
34541592	8	3	with	cross-validation	1328:1343	arg1	experiments					1360:1370	pyrolysis experiments	1350:1370	pyrolysis experiments	1350:1370	The prediction of these thermodynamic properties and the reaction enthalpies will benefit further computational studies and cross-validation with pyrolysis experiments.
34541592	0	4	theme	lignin	85:90	arg1	pyrolysis					92:100	hardwood model lignin pyrolysis	70:100	hardwood model lignin pyrolysis	70:100	Isolating key reaction energetics and thermodynamic properties during hardwood model lignin pyrolysis.
34541592	7	5	theme	formation	1093:1101	arg1	enthalpy					1081:1088	enthalpy	1081:1088	enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K	1081:1201	In addition, we calculated standard thermodynamic properties, including enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K.
34541592	5	6	theme	density	866:872	arg1	calculations					898:909	mechanically based density functional theory (DFT) calculations	847:909	mechanically based density functional theory (DFT) calculations	847:909	To accomplish this, we utilized a composite method using molecular mechanics based conformational sampling and quantum mechanically based density functional theory (DFT) calculations.
34541592	3	7	theme	representative	479:492	arg1	model					494:498	this representative model	474:498	this representative model	474:498	A lignin model of this size is potentially more representative of the polymer in angiosperms; therefore, we used this representative model to examine the behavior of hardwood lignin during the initial steps of pyrolysis.
34541592	6	8	theme	dissociation	960:971	arg1	BDE					985:987	BDE	985:987	BDE	985:987	Our key results show marked differences in bond dissociation enthalpies (BDE) with the position.
34541592	6	8	theme	dissociation	960:971	arg1	enthalpies					973:982	bond dissociation enthalpies	955:982	bond dissociation enthalpies (BDE) with the position	955:1006	Our key results show marked differences in bond dissociation enthalpies (BDE) with the position.
34541592	3	9	theme	initial	554:560	arg1	steps					562:566	the initial steps	550:566	the initial steps of pyrolysis	550:579	A lignin model of this size is potentially more representative of the polymer in angiosperms; therefore, we used this representative model to examine the behavior of hardwood lignin during the initial steps of pyrolysis.
34541592	5	10	theme	based	860:864	arg1	calculations					898:909	mechanically based density functional theory (DFT) calculations	847:909	mechanically based density functional theory (DFT) calculations	847:909	To accomplish this, we utilized a composite method using molecular mechanics based conformational sampling and quantum mechanically based density functional theory (DFT) calculations.
34541592	6	11	theme	bond	955:958	arg1	BDE					985:987	BDE	985:987	BDE	985:987	Our key results show marked differences in bond dissociation enthalpies (BDE) with the position.
34541592	6	11	theme	bond	955:958	arg1	enthalpies					973:982	bond dissociation enthalpies	955:982	bond dissociation enthalpies (BDE) with the position	955:1006	Our key results show marked differences in bond dissociation enthalpies (BDE) with the position.
34541592	7	12	theme	energy	1143:1148	arg1	enthalpy					1081:1088	enthalpy	1081:1088	enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K	1081:1201	In addition, we calculated standard thermodynamic properties, including enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K.
34541592	7	13	dep	K	1201:1201	arg1	to					1193:1194	to	1193:1194	to	1193:1194	In addition, we calculated standard thermodynamic properties, including enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K.
34541592	2	14	theme	lignin	282:287	arg1	oligomer					289:296	a lignin oligomer	280:296	a lignin oligomer consisting of 10 syringyl units linked through 9 β-O-4' bonds	280:358	In the current work we have modeled a lignin oligomer consisting of 10 syringyl units linked through 9 β-O-4' bonds.
34541592	4	15	theme	bond	676:679	arg1	cleavage					681:688	bond cleavage	676:688	bond cleavage	676:688	Using this oligomer, the present work aims to determine if and how the reaction enthalpies of bond cleavage vary with positions within the chain.
34541592	7	16	theme	free	1138:1141	arg1	energy					1143:1148	Gibbs free energy	1132:1148	Gibbs free energy	1132:1148	In addition, we calculated standard thermodynamic properties, including enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K.
34541592	9	17	theme	lignin	1447:1452	arg1	pyrolysis					1454:1462	lignin pyrolysis	1447:1462	lignin pyrolysis	1447:1462	Overall, the results demonstrate the utility of a better understanding of lignin pyrolysis for its effective valorization.
34541592	5	18	theme	molecular	785:793	arg1	sampling					826:833	molecular mechanics based conformational sampling	785:833	molecular mechanics based conformational sampling	785:833	To accomplish this, we utilized a composite method using molecular mechanics based conformational sampling and quantum mechanically based density functional theory (DFT) calculations.
34541592	8	19	theme	enthalpies	1270:1279	arg1	prediction					1208:1217	The prediction	1204:1217	The prediction of these thermodynamic properties and the reaction enthalpies	1204:1279	The prediction of these thermodynamic properties and the reaction enthalpies will benefit further computational studies and cross-validation with pyrolysis experiments.
34541592	5	20	theme	conformational	811:824	arg1	sampling					826:833	molecular mechanics based conformational sampling	785:833	molecular mechanics based conformational sampling	785:833	To accomplish this, we utilized a composite method using molecular mechanics based conformational sampling and quantum mechanically based density functional theory (DFT) calculations.
34541592	7	21	theme	heat	1104:1107	arg1	capacity					1109:1116	heat capacity	1104:1116	heat capacity	1104:1116	In addition, we calculated standard thermodynamic properties, including enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K.
34541592	5	22	theme	functional	874:883	arg1	calculations					898:909	mechanically based density functional theory (DFT) calculations	847:909	mechanically based density functional theory (DFT) calculations	847:909	To accomplish this, we utilized a composite method using molecular mechanics based conformational sampling and quantum mechanically based density functional theory (DFT) calculations.
34541592	7	23	theme	capacity	1109:1116	arg1	enthalpy					1081:1088	enthalpy	1081:1088	enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K	1081:1201	In addition, we calculated standard thermodynamic properties, including enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K.
34541592	0	24	theme	key	10:12	arg1	energetics					23:32	key reaction energetics	10:32	key reaction energetics	10:32	Isolating key reaction energetics and thermodynamic properties during hardwood model lignin pyrolysis.
34541592	5	25	theme	based	805:809	arg1	sampling					826:833	molecular mechanics based conformational sampling	785:833	molecular mechanics based conformational sampling	785:833	To accomplish this, we utilized a composite method using molecular mechanics based conformational sampling and quantum mechanically based density functional theory (DFT) calculations.
34541592	7	26	theme	standard	1036:1043	arg1	properties					1059:1068	standard thermodynamic properties	1036:1068	standard thermodynamic properties	1036:1068	In addition, we calculated standard thermodynamic properties, including enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K.
34541592	7	26	theme	standard	1036:1043	arg1	enthalpy					1081:1088	enthalpy	1081:1088	enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K	1081:1201	In addition, we calculated standard thermodynamic properties, including enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K.
34541592	7	27	from	K	1201:1201	arg1	range					1161:1165	a wide range	1154:1165	a wide range of temperatures from 25 K to 1000 K	1154:1201	In addition, we calculated standard thermodynamic properties, including enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K.
34541592	7	27	from	K	1201:1201	arg1	temperatures					1170:1181	temperatures	1170:1181	temperatures from 25 K to 1000 K	1170:1201	In addition, we calculated standard thermodynamic properties, including enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K.
34541592	2	28	theme	syringyl	315:322	arg1	units					324:328	10 syringyl units	312:328	10 syringyl units linked through 9 β-O-4' bonds	312:358	In the current work we have modeled a lignin oligomer consisting of 10 syringyl units linked through 9 β-O-4' bonds.
34541592	7	29	theme	Gibbs	1132:1136	arg1	energy					1143:1148	Gibbs free energy	1132:1148	Gibbs free energy	1132:1148	In addition, we calculated standard thermodynamic properties, including enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K.
34541592	7	30	theme	thermodynamic	1045:1057	arg1	properties					1059:1068	standard thermodynamic properties	1036:1068	standard thermodynamic properties	1036:1068	In addition, we calculated standard thermodynamic properties, including enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K.
34541592	7	30	theme	thermodynamic	1045:1057	arg1	enthalpy					1081:1088	enthalpy	1081:1088	enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K	1081:1201	In addition, we calculated standard thermodynamic properties, including enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K.
34541592	1	31	theme	Computational	103:115	arg1	studies					117:123	Computational studies	103:123	Computational studies on the pyrolysis of lignin using electronic structure methods	103:185	Computational studies on the pyrolysis of lignin using electronic structure methods have been largely limited to dimeric or trimeric models.
34541592	5	32	theme	composite	762:770	arg1	method					772:777	a composite method	760:777	a composite method using molecular mechanics based conformational sampling and quantum mechanically based density functional theory (DFT) calculations	760:909	To accomplish this, we utilized a composite method using molecular mechanics based conformational sampling and quantum mechanically based density functional theory (DFT) calculations.
34541592	6	33	theme	marked	933:938	arg1	differences					940:950	marked differences	933:950	marked differences in bond dissociation enthalpies (BDE) with the position	933:1006	Our key results show marked differences in bond dissociation enthalpies (BDE) with the position.
34541592	3	34	theme	hardwood	527:534	arg1	lignin					536:541	hardwood lignin	527:541	hardwood lignin	527:541	A lignin model of this size is potentially more representative of the polymer in angiosperms; therefore, we used this representative model to examine the behavior of hardwood lignin during the initial steps of pyrolysis.
34541592	9	35	theme	better	1423:1428	arg1	understanding					1430:1442	a better understanding	1421:1442	a better understanding of lignin pyrolysis	1421:1462	Overall, the results demonstrate the utility of a better understanding of lignin pyrolysis for its effective valorization.
34541592	3	36	theme	lignin	363:368	arg1	model					370:374	A lignin model	361:374	A lignin model of this size	361:387	A lignin model of this size is potentially more representative of the polymer in angiosperms; therefore, we used this representative model to examine the behavior of hardwood lignin during the initial steps of pyrolysis.
34541592	3	36	theme	lignin	363:368	arg1	representative					409:422	representative	409:422	representative	409:422	A lignin model of this size is potentially more representative of the polymer in angiosperms; therefore, we used this representative model to examine the behavior of hardwood lignin during the initial steps of pyrolysis.
34541592	9	37	theme	understanding	1430:1442	arg1	utility					1410:1416	the utility	1406:1416	the utility of a better understanding of lignin pyrolysis for its effective valorization	1406:1493	Overall, the results demonstrate the utility of a better understanding of lignin pyrolysis for its effective valorization.
34541592	8	38	theme	further	1294:1300	arg1	studies					1316:1322	further computational studies	1294:1322	further computational studies	1294:1322	The prediction of these thermodynamic properties and the reaction enthalpies will benefit further computational studies and cross-validation with pyrolysis experiments.
34541592	3	39	theme	lignin	536:541	arg1	behavior					515:522	the behavior	511:522	the behavior of hardwood lignin during the initial steps of pyrolysis	511:579	A lignin model of this size is potentially more representative of the polymer in angiosperms; therefore, we used this representative model to examine the behavior of hardwood lignin during the initial steps of pyrolysis.
34541592	1	40	theme	dimeric	216:222	arg1	models					236:241	dimeric or trimeric models	216:241	dimeric or trimeric models	216:241	Computational studies on the pyrolysis of lignin using electronic structure methods have been largely limited to dimeric or trimeric models.
34541592	0	41	theme	thermodynamic	38:50	arg1	properties					52:61	thermodynamic properties	38:61	thermodynamic properties	38:61	Isolating key reaction energetics and thermodynamic properties during hardwood model lignin pyrolysis.
34541592	6	42	from	differences	940:950	arg1	BDE					985:987	BDE	985:987	BDE	985:987	Our key results show marked differences in bond dissociation enthalpies (BDE) with the position.
34541592	6	42	from	differences	940:950	arg1	enthalpies					973:982	bond dissociation enthalpies	955:982	bond dissociation enthalpies (BDE) with the position	955:1006	Our key results show marked differences in bond dissociation enthalpies (BDE) with the position.
34541592	5	43	theme	mechanics	795:803	arg1	sampling					826:833	molecular mechanics based conformational sampling	785:833	molecular mechanics based conformational sampling	785:833	To accomplish this, we utilized a composite method using molecular mechanics based conformational sampling and quantum mechanically based density functional theory (DFT) calculations.
34541592	8	44	theme	pyrolysis	1350:1358	arg1	experiments					1360:1370	pyrolysis experiments	1350:1370	pyrolysis experiments	1350:1370	The prediction of these thermodynamic properties and the reaction enthalpies will benefit further computational studies and cross-validation with pyrolysis experiments.
34541592	8	45	theme	thermodynamic	1228:1240	arg1	properties					1242:1251	these thermodynamic properties	1222:1251	these thermodynamic properties	1222:1251	The prediction of these thermodynamic properties and the reaction enthalpies will benefit further computational studies and cross-validation with pyrolysis experiments.
34541592	3	46	theme	polymer	431:437	arg1	model					370:374	A lignin model	361:374	A lignin model of this size	361:387	A lignin model of this size is potentially more representative of the polymer in angiosperms; therefore, we used this representative model to examine the behavior of hardwood lignin during the initial steps of pyrolysis.
34541592	3	46	theme	polymer	431:437	arg1	representative					409:422	representative	409:422	representative	409:422	A lignin model of this size is potentially more representative of the polymer in angiosperms; therefore, we used this representative model to examine the behavior of hardwood lignin during the initial steps of pyrolysis.
34541592	0	47	theme	reaction	14:21	arg1	energetics					23:32	key reaction energetics	10:32	key reaction energetics	10:32	Isolating key reaction energetics and thermodynamic properties during hardwood model lignin pyrolysis.
34541592	6	48	theme	key	916:918	arg1	results					920:926	Our key results	912:926	Our key results	912:926	Our key results show marked differences in bond dissociation enthalpies (BDE) with the position.
34541592	8	49	theme	properties	1242:1251	arg1	prediction					1208:1217	The prediction	1204:1217	The prediction of these thermodynamic properties and the reaction enthalpies	1204:1279	The prediction of these thermodynamic properties and the reaction enthalpies will benefit further computational studies and cross-validation with pyrolysis experiments.
34541592	3	50	theme	pyrolysis	571:579	arg1	steps					562:566	the initial steps	550:566	the initial steps of pyrolysis	550:579	A lignin model of this size is potentially more representative of the polymer in angiosperms; therefore, we used this representative model to examine the behavior of hardwood lignin during the initial steps of pyrolysis.
34541592	3	51	from	polymer	431:437	arg1	angiosperms					442:452	angiosperms	442:452	angiosperms	442:452	A lignin model of this size is potentially more representative of the polymer in angiosperms; therefore, we used this representative model to examine the behavior of hardwood lignin during the initial steps of pyrolysis.
34541592	1	52	theme	trimeric	227:234	arg1	models					236:241	dimeric or trimeric models	216:241	dimeric or trimeric models	216:241	Computational studies on the pyrolysis of lignin using electronic structure methods have been largely limited to dimeric or trimeric models.
34541592	7	53	theme	temperatures	1170:1181	arg1	range					1161:1165	a wide range	1154:1165	a wide range of temperatures from 25 K to 1000 K	1154:1201	In addition, we calculated standard thermodynamic properties, including enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K.
34541592	3	54	used	used	469:472	arg2	we					466:467	we	466:467	we	466:467	A lignin model of this size is potentially more representative of the polymer in angiosperms; therefore, we used this representative model to examine the behavior of hardwood lignin during the initial steps of pyrolysis.
34541592	8	55	theme	computational	1302:1314	arg1	studies					1316:1322	further computational studies	1294:1322	further computational studies	1294:1322	The prediction of these thermodynamic properties and the reaction enthalpies will benefit further computational studies and cross-validation with pyrolysis experiments.
34541592	5	56	theme	theory	885:890	arg1	calculations					898:909	mechanically based density functional theory (DFT) calculations	847:909	mechanically based density functional theory (DFT) calculations	847:909	To accomplish this, we utilized a composite method using molecular mechanics based conformational sampling and quantum mechanically based density functional theory (DFT) calculations.
34541592	3	57	theme	size	384:387	arg1	model					370:374	A lignin model	361:374	A lignin model of this size	361:387	A lignin model of this size is potentially more representative of the polymer in angiosperms; therefore, we used this representative model to examine the behavior of hardwood lignin during the initial steps of pyrolysis.
34541592	3	57	theme	size	384:387	arg1	representative					409:422	representative	409:422	representative	409:422	A lignin model of this size is potentially more representative of the polymer in angiosperms; therefore, we used this representative model to examine the behavior of hardwood lignin during the initial steps of pyrolysis.
34541592	5	58	dep	sampling	826:833	arg1	calculations					898:909	mechanically based density functional theory (DFT) calculations	847:909	mechanically based density functional theory (DFT) calculations	847:909	To accomplish this, we utilized a composite method using molecular mechanics based conformational sampling and quantum mechanically based density functional theory (DFT) calculations.
34541592	9	59	theme	pyrolysis	1454:1462	arg1	understanding					1430:1442	a better understanding	1421:1442	a better understanding of lignin pyrolysis	1421:1462	Overall, the results demonstrate the utility of a better understanding of lignin pyrolysis for its effective valorization.
34541592	1	60	from	studies	117:123	arg1	pyrolysis					132:140	the pyrolysis	128:140	the pyrolysis of lignin using electronic structure methods	128:185	Computational studies on the pyrolysis of lignin using electronic structure methods have been largely limited to dimeric or trimeric models.
34541592	7	61	theme	entropy	1119:1125	arg1	enthalpy					1081:1088	enthalpy	1081:1088	enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K	1081:1201	In addition, we calculated standard thermodynamic properties, including enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K.
34541592	7	62	from	range	1161:1165	arg1	K					1201:1201	25 K to 1000 K	1188:1201	25 K to 1000 K	1188:1201	In addition, we calculated standard thermodynamic properties, including enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K.
34541592	4	63	dep	if	638:639	arg1	vary					690:693	vary	690:693	vary with positions within the chain	690:725	Using this oligomer, the present work aims to determine if and how the reaction enthalpies of bond cleavage vary with positions within the chain.
34541592	4	64	theme	cleavage	681:688	arg1	enthalpies					662:671	the reaction enthalpies	649:671	the reaction enthalpies of bond cleavage	649:688	Using this oligomer, the present work aims to determine if and how the reaction enthalpies of bond cleavage vary with positions within the chain.
34541592	5	65	used	utilized	751:758	arg2	we					748:749	we	748:749	we	748:749	To accomplish this, we utilized a composite method using molecular mechanics based conformational sampling and quantum mechanically based density functional theory (DFT) calculations.
34541592	1	66	theme	lignin	145:150	arg1	pyrolysis					132:140	the pyrolysis	128:140	the pyrolysis of lignin using electronic structure methods	128:185	Computational studies on the pyrolysis of lignin using electronic structure methods have been largely limited to dimeric or trimeric models.
34541592	8	67	theme	reaction	1261:1268	arg1	enthalpies					1270:1279	the reaction enthalpies	1257:1279	the reaction enthalpies	1257:1279	The prediction of these thermodynamic properties and the reaction enthalpies will benefit further computational studies and cross-validation with pyrolysis experiments.
34541592	5	68	theme	DFT	893:895	arg1	calculations					898:909	mechanically based density functional theory (DFT) calculations	847:909	mechanically based density functional theory (DFT) calculations	847:909	To accomplish this, we utilized a composite method using molecular mechanics based conformational sampling and quantum mechanically based density functional theory (DFT) calculations.
34541592	0	69	theme	model	79:83	arg1	pyrolysis					92:100	hardwood model lignin pyrolysis	70:100	hardwood model lignin pyrolysis	70:100	Isolating key reaction energetics and thermodynamic properties during hardwood model lignin pyrolysis.
34541592	4	70	theme	present	607:613	arg1	work					615:618	the present work	603:618	the present work	603:618	Using this oligomer, the present work aims to determine if and how the reaction enthalpies of bond cleavage vary with positions within the chain.
34541592	6	71	with	enthalpies	973:982	arg1	position					999:1006	the position	995:1006	the position	995:1006	Our key results show marked differences in bond dissociation enthalpies (BDE) with the position.
34541592	9	72	theme	effective	1472:1480	arg1	valorization					1482:1493	its effective valorization	1468:1493	its effective valorization	1468:1493	Overall, the results demonstrate the utility of a better understanding of lignin pyrolysis for its effective valorization.
34541592	7	73	theme	K	1191:1191	arg1	K					1201:1201	25 K to 1000 K	1188:1201	25 K to 1000 K	1188:1201	In addition, we calculated standard thermodynamic properties, including enthalpy of formation, heat capacity, entropy, and Gibbs free energy for a wide range of temperatures from 25 K to 1000 K.
34541592	0	74	theme	hardwood	70:77	arg1	pyrolysis					92:100	hardwood model lignin pyrolysis	70:100	hardwood model lignin pyrolysis	70:100	Isolating key reaction energetics and thermodynamic properties during hardwood model lignin pyrolysis.
34541592	2	75	theme	current	251:257	arg1	work					259:262	the current work	247:262	the current work	247:262	In the current work we have modeled a lignin oligomer consisting of 10 syringyl units linked through 9 β-O-4' bonds.
34541592	1	76	theme	electronic	158:167	arg1	methods					179:185	electronic structure methods	158:185	electronic structure methods	158:185	Computational studies on the pyrolysis of lignin using electronic structure methods have been largely limited to dimeric or trimeric models.
32971329	5	0	theme	sorbitol	740:747	arg1	yield					749:753	66% sorbitol yield	736:753	The highest 66% sorbitol yield	724:753	The highest 66% sorbitol yield was observed in the presence of 3%Ru-PW/ZrO2 activated at 550 °C and 1/1 of weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure.
32971329	2	1	theme	main	352:355	arg1	parameters					357:366	The main parameters	348:366	The main parameters	348:366	The main parameters impacted the reaction rate and yield of sorbitol, i.e. reaction conditions and type of catalyst were investigated.
32971329	1	2	theme	Ru-containing	246:258	arg1	composites					260:269	Ru-containing composites	246:269	Ru-containing composites based on H3PW12O40 supported on ZrO2 and Nb2O5 (Ru-PW/ZrO2 and Ru-PW/Nb2O5)	246:345	The aim of this work was to study the one-pot synthesis of sorbitol via hydrolysis-hydrogenation of cellulose in the presence of Ru-containing composites based on H3PW12O40 supported on ZrO2 and Nb2O5 (Ru-PW/ZrO2 and Ru-PW/Nb2O5).
32971329	5	3	theme	cellulose/catalyst	847:864	arg1	ratio					838:842	weight ratio	831:842	weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure	831:897	The highest 66% sorbitol yield was observed in the presence of 3%Ru-PW/ZrO2 activated at 550 °C and 1/1 of weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure.
32971329	4	4	theme	acid	683:686	arg1	sites					688:692	acid sites	683:692	acid sites	683:692	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	1	5	theme	composites	260:269	arg1	presence					234:241	the presence	230:241	the presence of Ru-containing composites based on H3PW12O40 supported on ZrO2 and Nb2O5 (Ru-PW/ZrO2 and Ru-PW/Nb2O5)	230:345	The aim of this work was to study the one-pot synthesis of sorbitol via hydrolysis-hydrogenation of cellulose in the presence of Ru-containing composites based on H3PW12O40 supported on ZrO2 and Nb2O5 (Ru-PW/ZrO2 and Ru-PW/Nb2O5).
32971329	0	6	theme	composites	105:114	arg1	presence					79:86	the presence	75:86	the presence of Ru-containing composites	75:114	One-pot synthesis of sorbitol via hydrolysis-hydrogenation of cellulose in the presence of Ru-containing composites.
32971329	0	7	theme	Ru-containing	91:103	arg1	composites					105:114	Ru-containing composites	91:114	Ru-containing composites	91:114	One-pot synthesis of sorbitol via hydrolysis-hydrogenation of cellulose in the presence of Ru-containing composites.
32971329	4	8	theme	sites	688:692	arg1	size					698:701	size	698:701	size of Ru nanoparticles	698:721	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	4	8	theme	sites	688:692	arg1	amount					673:678	the amount	669:678	the amount of acid sites	669:692	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	4	8	theme	sites	688:692	arg1	sites					688:692	acid sites	683:692	acid sites	683:692	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	4	8	theme	sites	688:692	arg1	nanoparticles					709:721	Ru nanoparticles	706:721	Ru nanoparticles	706:721	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	4	8	theme	sites	688:692	arg1	properties					657:666	textural properties	648:666	textural properties	648:666	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	5	9	theme	hydrogen	881:888	arg1	cellulose/catalyst					847:864	cellulose/catalyst	847:864	cellulose/catalyst	847:864	The highest 66% sorbitol yield was observed in the presence of 3%Ru-PW/ZrO2 activated at 550 °C and 1/1 of weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure.
32971329	5	9	theme	hydrogen	881:888	arg1	pressure					890:897	7 MPa hydrogen pressure	875:897	7 MPa hydrogen pressure	875:897	The highest 66% sorbitol yield was observed in the presence of 3%Ru-PW/ZrO2 activated at 550 °C and 1/1 of weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure.
32971329	5	10	theme	7 MPa	875:879	arg1	cellulose/catalyst					847:864	cellulose/catalyst	847:864	cellulose/catalyst	847:864	The highest 66% sorbitol yield was observed in the presence of 3%Ru-PW/ZrO2 activated at 550 °C and 1/1 of weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure.
32971329	5	10	theme	7 MPa	875:879	arg1	pressure					890:897	7 MPa hydrogen pressure	875:897	7 MPa hydrogen pressure	875:897	The highest 66% sorbitol yield was observed in the presence of 3%Ru-PW/ZrO2 activated at 550 °C and 1/1 of weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure.
32971329	1	11	theme	one-pot	155:161	arg1	synthesis					163:171	the one-pot synthesis	151:171	the one-pot synthesis of sorbitol	151:183	The aim of this work was to study the one-pot synthesis of sorbitol via hydrolysis-hydrogenation of cellulose in the presence of Ru-containing composites based on H3PW12O40 supported on ZrO2 and Nb2O5 (Ru-PW/ZrO2 and Ru-PW/Nb2O5).
32971329	5	12	theme	highest	728:734	arg1	yield					749:753	66% sorbitol yield	736:753	The highest 66% sorbitol yield	724:753	The highest 66% sorbitol yield was observed in the presence of 3%Ru-PW/ZrO2 activated at 550 °C and 1/1 of weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure.
32971329	0	13	from	hydrolysis-hydrogenation	34:57	arg1	presence					79:86	the presence	75:86	the presence of Ru-containing composites	75:114	One-pot synthesis of sorbitol via hydrolysis-hydrogenation of cellulose in the presence of Ru-containing composites.
32971329	6	14	theme	reaction	949:956	arg1	cycles					935:940	three cycles	929:940	three cycles of the reaction	929:956	This catalyst was stable for three cycles of the reaction without lost of it's activity.
32971329	5	15	located	observed	759:766	arg2	yield					749:753	66% sorbitol yield	736:753	The highest 66% sorbitol yield	724:753	The highest 66% sorbitol yield was observed in the presence of 3%Ru-PW/ZrO2 activated at 550 °C and 1/1 of weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure.
32971329	5	15	located	observed	759:766	arg1	presence					775:782	the presence	771:782	the presence of 3%Ru-PW/ZrO2 activated at 550 °C and 1/1 of weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure	771:897	The highest 66% sorbitol yield was observed in the presence of 3%Ru-PW/ZrO2 activated at 550 °C and 1/1 of weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure.
32971329	4	16	theme	textural	648:655	arg1	properties					657:666	textural properties	648:666	textural properties	648:666	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	4	16	theme	textural	648:655	arg1	amount					673:678	the amount	669:678	the amount of acid sites	669:692	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	4	16	theme	textural	648:655	arg1	sites					688:692	acid sites	683:692	acid sites	683:692	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	4	16	theme	textural	648:655	arg1	nanoparticles					709:721	Ru nanoparticles	706:721	Ru nanoparticles	706:721	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	4	16	theme	textural	648:655	arg1	size					698:701	size	698:701	size of Ru nanoparticles	698:721	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	1	17	dep	Nb2O5	312:316	arg1	Ru-PW/Nb2O5					334:344	Ru-PW/Nb2O5	334:344	Ru-PW/Nb2O5	334:344	The aim of this work was to study the one-pot synthesis of sorbitol via hydrolysis-hydrogenation of cellulose in the presence of Ru-containing composites based on H3PW12O40 supported on ZrO2 and Nb2O5 (Ru-PW/ZrO2 and Ru-PW/Nb2O5).
32971329	1	17	dep	Nb2O5	312:316	arg1	Ru-PW/ZrO2					319:328	Ru-PW/ZrO2	319:328	Ru-PW/ZrO2	319:328	The aim of this work was to study the one-pot synthesis of sorbitol via hydrolysis-hydrogenation of cellulose in the presence of Ru-containing composites based on H3PW12O40 supported on ZrO2 and Nb2O5 (Ru-PW/ZrO2 and Ru-PW/Nb2O5).
32971329	2	18	theme	reaction	381:388	arg1	rate					390:393	reaction rate	381:393	reaction rate	381:393	The main parameters impacted the reaction rate and yield of sorbitol, i.e. reaction conditions and type of catalyst were investigated.
32971329	0	19	theme	One-pot	0:6	arg1	synthesis					8:16	One-pot synthesis	0:16	One-pot synthesis of sorbitol via hydrolysis-hydrogenation of cellulose in the presence of Ru-containing composites.	0:115	One-pot synthesis of sorbitol via hydrolysis-hydrogenation of cellulose in the presence of Ru-containing composites.
32971329	4	20	theme	sorbitol	550:557	arg1	yield					541:545	The yield	537:545	The yield of sorbitol	537:557	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	2	21	dep	conditions	432:441	arg1	i.e.					418:421	i.e.	418:421	i.e.	418:421	The main parameters impacted the reaction rate and yield of sorbitol, i.e. reaction conditions and type of catalyst were investigated.
32971329	4	22	theme	nanoparticles	709:721	arg1	size					698:701	size	698:701	size of Ru nanoparticles	698:721	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	4	22	theme	nanoparticles	709:721	arg1	amount					673:678	the amount	669:678	the amount of acid sites	669:692	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	4	22	theme	nanoparticles	709:721	arg1	sites					688:692	acid sites	683:692	acid sites	683:692	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	4	22	theme	nanoparticles	709:721	arg1	nanoparticles					709:721	Ru nanoparticles	706:721	Ru nanoparticles	706:721	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	4	22	theme	nanoparticles	709:721	arg1	properties					657:666	textural properties	648:666	textural properties	648:666	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	1	23	theme	sorbitol	176:183	arg1	synthesis					163:171	the one-pot synthesis	151:171	the one-pot synthesis of sorbitol	151:183	The aim of this work was to study the one-pot synthesis of sorbitol via hydrolysis-hydrogenation of cellulose in the presence of Ru-containing composites based on H3PW12O40 supported on ZrO2 and Nb2O5 (Ru-PW/ZrO2 and Ru-PW/Nb2O5).
32971329	0	24	theme	sorbitol	21:28	arg1	synthesis					8:16	One-pot synthesis	0:16	One-pot synthesis of sorbitol via hydrolysis-hydrogenation of cellulose in the presence of Ru-containing composites.	0:115	One-pot synthesis of sorbitol via hydrolysis-hydrogenation of cellulose in the presence of Ru-containing composites.
32971329	4	25	theme	PW/Nb2O5	624:631	arg1	temperature					597:607	the activation temperature	582:607	the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles	582:721	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	5	26	theme	%	788:788	arg1	Ru-PW/ZrO2					789:798	3%Ru-PW/ZrO2	787:798	3%Ru-PW/ZrO2 activated at 550 °C and 1/1 of weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure	787:897	The highest 66% sorbitol yield was observed in the presence of 3%Ru-PW/ZrO2 activated at 550 °C and 1/1 of weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure.
32971329	5	27	theme	3	787:787	arg1	%					788:788	%	788:788	%	788:788	The highest 66% sorbitol yield was observed in the presence of 3%Ru-PW/ZrO2 activated at 550 °C and 1/1 of weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure.
32971329	2	28	theme	sorbitol	408:415	arg1	rate					390:393	reaction rate	381:393	reaction rate	381:393	The main parameters impacted the reaction rate and yield of sorbitol, i.e. reaction conditions and type of catalyst were investigated.
32971329	2	28	theme	sorbitol	408:415	arg1	yield					399:403	yield	399:403	yield	399:403	The main parameters impacted the reaction rate and yield of sorbitol, i.e. reaction conditions and type of catalyst were investigated.
32971329	5	29	theme	weight	831:836	arg1	ratio					838:842	weight ratio	831:842	weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure	831:897	The highest 66% sorbitol yield was observed in the presence of 3%Ru-PW/ZrO2 activated at 550 °C and 1/1 of weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure.
32971329	2	30	theme	catalyst	455:462	arg1	type					447:450	type	447:450	type of catalyst	447:462	The main parameters impacted the reaction rate and yield of sorbitol, i.e. reaction conditions and type of catalyst were investigated.
32971329	2	31	theme	reaction	423:430	arg1	conditions					432:441	reaction conditions	423:441	reaction conditions	423:441	The main parameters impacted the reaction rate and yield of sorbitol, i.e. reaction conditions and type of catalyst were investigated.
32971329	1	32	theme	cellulose	217:225	arg1	hydrolysis-hydrogenation					189:212	hydrolysis-hydrogenation	189:212	hydrolysis-hydrogenation of cellulose	189:225	The aim of this work was to study the one-pot synthesis of sorbitol via hydrolysis-hydrogenation of cellulose in the presence of Ru-containing composites based on H3PW12O40 supported on ZrO2 and Nb2O5 (Ru-PW/ZrO2 and Ru-PW/Nb2O5).
32971329	0	33	theme	cellulose	62:70	arg1	hydrolysis-hydrogenation					34:57	hydrolysis-hydrogenation	34:57	hydrolysis-hydrogenation of cellulose in the presence of Ru-containing composites	34:114	One-pot synthesis of sorbitol via hydrolysis-hydrogenation of cellulose in the presence of Ru-containing composites.
32971329	3	34	theme	Ru-PW/ZrO2	483:492	arg1	systems					494:500	Ru-PW/ZrO2 systems	483:500	Ru-PW/ZrO2 systems	483:500	Ru-PW/ZrO2 systems were more active than Ru-PW/Nb2O5.
32971329	6	35	dep	stable	918:923	arg1	activity					979:986	it's activity	974:986	it's activity	974:986	This catalyst was stable for three cycles of the reaction without lost of it's activity.
32971329	4	36	theme	Ru	706:707	arg1	nanoparticles					709:721	Ru nanoparticles	706:721	Ru nanoparticles	706:721	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	1	37	theme	work	133:136	arg1	aim					121:123	The aim	117:123	The aim of this work	117:136	The aim of this work was to study the one-pot synthesis of sorbitol via hydrolysis-hydrogenation of cellulose in the presence of Ru-containing composites based on H3PW12O40 supported on ZrO2 and Nb2O5 (Ru-PW/ZrO2 and Ru-PW/Nb2O5).
32971329	4	38	theme	activation	586:595	arg1	temperature					597:607	the activation temperature	582:607	the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles	582:721	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	5	39	theme	66	736:737	arg1	%					738:738	%	738:738	%	738:738	The highest 66% sorbitol yield was observed in the presence of 3%Ru-PW/ZrO2 activated at 550 °C and 1/1 of weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure.
32971329	5	40	theme	Ru-PW/ZrO2	789:798	arg1	presence					775:782	the presence	771:782	the presence of 3%Ru-PW/ZrO2 activated at 550 °C and 1/1 of weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure	771:897	The highest 66% sorbitol yield was observed in the presence of 3%Ru-PW/ZrO2 activated at 550 °C and 1/1 of weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure.
32971329	5	41	theme	ratio	838:842	arg1	1/1					824:826	1/1	824:826	1/1	824:826	The highest 66% sorbitol yield was observed in the presence of 3%Ru-PW/ZrO2 activated at 550 °C and 1/1 of weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure.
32971329	5	41	theme	ratio	838:842	arg1	550 °C					813:818	550 °C	813:818	550 °C	813:818	The highest 66% sorbitol yield was observed in the presence of 3%Ru-PW/ZrO2 activated at 550 °C and 1/1 of weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure.
32971329	4	42	theme	PW/ZrO2	612:618	arg1	temperature					597:607	the activation temperature	582:607	the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles	582:721	The yield of sorbitol was found to depend on the activation temperature of PW/ZrO2 and PW/Nb2O5 which affected textural properties, the amount of acid sites and size of Ru nanoparticles.
32971329	5	43	theme	%	738:738	arg1	yield					749:753	66% sorbitol yield	736:753	The highest 66% sorbitol yield	724:753	The highest 66% sorbitol yield was observed in the presence of 3%Ru-PW/ZrO2 activated at 550 °C and 1/1 of weight ratio of cellulose/catalyst, 180 °C, 7 MPa hydrogen pressure.
32971329	2	44	dep	rate	390:393	arg1	the					377:379	the	377:379	the	377:379	The main parameters impacted the reaction rate and yield of sorbitol, i.e. reaction conditions and type of catalyst were investigated.
33338254	0	0	from	lactuca	45:51	arg1	Glycoproteins					21:33	Arabinogalactan-like Glycoproteins	0:33	Arabinogalactan-like Glycoproteins from Ulva lactuca (Chlorophyta)	0:65	Arabinogalactan-like Glycoproteins from Ulva lactuca (Chlorophyta) Show Unique Features Compared to Land Plants AGPs.
33338254	8	1	theme	new	1727:1729	arg1	insight					1731:1737	new insight	1727:1737	new insight into the evolution of the plant cell wall	1727:1779	The exceptional structure of the Ulva lactuca AGP-like glycoproteins implies a specialized adaptation to the marine environment and might bring new insight into the evolution of the plant cell wall.
33338254	6	2	theme	Ulva	1356:1359	arg1	glycoproteins					1378:1390	the Ulva lactuca AGP-like glycoproteins	1352:1390	the Ulva lactuca AGP-like glycoproteins	1352:1390	While the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent showed a similarity between algal and land plant AGP-like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP-like glycoproteins.
33338254	4	3	theme	described	912:920	arg1	AGPs					943:946	scarcely described Solanum lycopersicum AGPs	903:946	scarcely described Solanum lycopersicum AGPs	903:946	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	7	4	theme	3-O-methyl-hexose	1520:1536	arg1	monosaccharides					1459:1473	the most prevalent monosaccharides	1440:1473	the most prevalent monosaccharides	1440:1473	Surprisingly, arabinose and galactose were not the most prevalent monosaccharides and the most outstanding was the presence of 3-O-methyl-hexose, which has never been described in the AGPs.
33338254	7	4	theme	3-O-methyl-hexose	1520:1536	arg1	galactose					1421:1429	galactose	1421:1429	galactose	1421:1429	Surprisingly, arabinose and galactose were not the most prevalent monosaccharides and the most outstanding was the presence of 3-O-methyl-hexose, which has never been described in the AGPs.
33338254	7	4	theme	3-O-methyl-hexose	1520:1536	arg1	outstanding					1488:1498	outstanding	1488:1498	outstanding	1488:1498	Surprisingly, arabinose and galactose were not the most prevalent monosaccharides and the most outstanding was the presence of 3-O-methyl-hexose, which has never been described in the AGPs.
33338254	7	4	theme	3-O-methyl-hexose	1520:1536	arg1	presence					1508:1515	the presence	1504:1515	the presence of 3-O-methyl-hexose, which has never been described in the AGPs	1504:1580	Surprisingly, arabinose and galactose were not the most prevalent monosaccharides and the most outstanding was the presence of 3-O-methyl-hexose, which has never been described in the AGPs.
33338254	7	4	theme	3-O-methyl-hexose	1520:1536	arg1	arabinose					1407:1415	arabinose	1407:1415	arabinose	1407:1415	Surprisingly, arabinose and galactose were not the most prevalent monosaccharides and the most outstanding was the presence of 3-O-methyl-hexose, which has never been described in the AGPs.
33338254	5	5	gly	glycoproteins	1100:1112	arg1	glycoproteins					1100:1112	AGP-like glycoproteins	1091:1112	AGP-like glycoproteins	1091:1112	The reactivity with primary anti-AGP antibodies as well as Yariv reagent showed a great variety between Ulva lactuca and Solanum lycopersicum AGP-like glycoproteins.
33338254	3	6	theme	various	532:538	arg1	applications					540:551	various applications	532:551	various applications	532:551	Ulva species have high economic potential since various applications were previously described including bioremediation, biofuel production, and as a source of bioactive compounds.
33338254	6	7	theme	unique	1328:1333	arg1	glycosylation					1335:1347	unique glycosylation	1328:1347	unique glycosylation of the Ulva lactuca AGP-like glycoproteins	1328:1390	While the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent showed a similarity between algal and land plant AGP-like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP-like glycoproteins.
33338254	0	8	theme	Arabinogalactan-like	0:19	arg1	Glycoproteins					21:33	Arabinogalactan-like Glycoproteins	0:33	Arabinogalactan-like Glycoproteins from Ulva lactuca (Chlorophyta)	0:65	Arabinogalactan-like Glycoproteins from Ulva lactuca (Chlorophyta) Show Unique Features Compared to Land Plants AGPs.
33338254	4	9	dep	Ulva	810:813	arg1	lactuca					815:821	lactuca	815:821	lactuca	815:821	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	4	10	theme	unique	882:887	arg1	glycoproteins					832:844	Ulva lactuca AGP-like glycoproteins	810:844	Ulva lactuca AGP-like glycoproteins	810:844	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	4	10	theme	unique	882:887	arg1	comparison					889:898	unique comparison	882:898	unique comparison to scarcely described Solanum lycopersicum AGPs	882:946	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	1	11	theme	plant	179:183	arg1	proteoglycans					195:207	plant cell wall proteoglycans	179:207	plant cell wall proteoglycans	179:207	Arabinogalactan proteins (AGPs) encompass a diverse group of plant cell wall proteoglycans, which play an essential role in plant development, signaling, plant-microbe interactions, and many others.
33338254	6	12	theme	Yariv	1204:1208	arg1	reagent					1210:1216	the β-d-glucosyl Yariv reagent	1187:1216	the β-d-glucosyl Yariv reagent	1187:1216	While the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent showed a similarity between algal and land plant AGP-like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP-like glycoproteins.
33338254	1	13	theme	wall	190:193	arg1	proteoglycans					195:207	plant cell wall proteoglycans	179:207	plant cell wall proteoglycans	179:207	Arabinogalactan proteins (AGPs) encompass a diverse group of plant cell wall proteoglycans, which play an essential role in plant development, signaling, plant-microbe interactions, and many others.
33338254	1	14	theme	Arabinogalactan	118:132	arg1	proteins					134:141	Arabinogalactan proteins	118:141	Arabinogalactan proteins (AGPs)	118:148	Arabinogalactan proteins (AGPs) encompass a diverse group of plant cell wall proteoglycans, which play an essential role in plant development, signaling, plant-microbe interactions, and many others.
33338254	1	14	theme	Arabinogalactan	118:132	arg1	AGPs					144:147	AGPs	144:147	AGPs	144:147	Arabinogalactan proteins (AGPs) encompass a diverse group of plant cell wall proteoglycans, which play an essential role in plant development, signaling, plant-microbe interactions, and many others.
33338254	6	15	theme	saccharide	1299:1308	arg1	analysis					1310:1317	neutral saccharide analysis	1291:1317	neutral saccharide analysis	1291:1317	While the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent showed a similarity between algal and land plant AGP-like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP-like glycoproteins.
33338254	6	16	dep	algal	1246:1250	arg1	glycoproteins					1276:1288	plant AGP-like glycoproteins	1261:1288	plant AGP-like glycoproteins	1261:1288	While the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent showed a similarity between algal and land plant AGP-like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP-like glycoproteins.
33338254	4	17	gly	glycoproteins	735:747	arg1	glycoproteins					735:747	AGP-like glycoproteins	726:747	AGP-like glycoproteins in Ulva species	726:763	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	3	18	theme	biofuel	605:611	arg1	bioremediation					589:602	bioremediation	589:602	bioremediation	589:602	Ulva species have high economic potential since various applications were previously described including bioremediation, biofuel production, and as a source of bioactive compounds.
33338254	3	18	theme	biofuel	605:611	arg1	production					613:622	biofuel production	605:622	biofuel production	605:622	Ulva species have high economic potential since various applications were previously described including bioremediation, biofuel production, and as a source of bioactive compounds.
33338254	4	19	theme	lycopersicum	930:941	arg1	AGPs					943:946	scarcely described Solanum lycopersicum AGPs	903:946	scarcely described Solanum lycopersicum AGPs	903:946	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	7	20	theme	prevalent	1449:1457	arg1	monosaccharides					1459:1473	the most prevalent monosaccharides	1440:1473	the most prevalent monosaccharides	1440:1473	Surprisingly, arabinose and galactose were not the most prevalent monosaccharides and the most outstanding was the presence of 3-O-methyl-hexose, which has never been described in the AGPs.
33338254	7	20	theme	prevalent	1449:1457	arg1	galactose					1421:1429	galactose	1421:1429	galactose	1421:1429	Surprisingly, arabinose and galactose were not the most prevalent monosaccharides and the most outstanding was the presence of 3-O-methyl-hexose, which has never been described in the AGPs.
33338254	7	20	theme	prevalent	1449:1457	arg1	arabinose					1407:1415	arabinose	1407:1415	arabinose	1407:1415	Surprisingly, arabinose and galactose were not the most prevalent monosaccharides and the most outstanding was the presence of 3-O-methyl-hexose, which has never been described in the AGPs.
33338254	6	21	gly	glycosylation	1335:1347	arg1	glycoproteins					1378:1390	the Ulva lactuca AGP-like glycoproteins	1352:1390	the Ulva lactuca AGP-like glycoproteins	1352:1390	While the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent showed a similarity between algal and land plant AGP-like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP-like glycoproteins.
33338254	6	22	theme	AGP-like	1267:1274	arg1	glycoproteins					1276:1288	plant AGP-like glycoproteins	1261:1288	plant AGP-like glycoproteins	1261:1288	While the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent showed a similarity between algal and land plant AGP-like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP-like glycoproteins.
33338254	4	23	theme	glycoproteins	735:747	arg1	confirmation					710:721	the first experimental confirmation	687:721	the first experimental confirmation of AGP-like glycoproteins in Ulva species	687:763	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	6	24	theme	AGP-like	1369:1376	arg1	glycoproteins					1378:1390	the Ulva lactuca AGP-like glycoproteins	1352:1390	the Ulva lactuca AGP-like glycoproteins	1352:1390	While the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent showed a similarity between algal and land plant AGP-like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP-like glycoproteins.
33338254	5	25	theme	primary	969:975	arg1	antibodies					986:995	primary anti-AGP antibodies	969:995	primary anti-AGP antibodies as well as Yariv reagent	969:1020	The reactivity with primary anti-AGP antibodies as well as Yariv reagent showed a great variety between Ulva lactuca and Solanum lycopersicum AGP-like glycoproteins.
33338254	4	26	theme	experimental	697:708	arg1	confirmation					710:721	the first experimental confirmation	687:721	the first experimental confirmation of AGP-like glycoproteins in Ulva species	687:763	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	3	27	contain	have	497:500	arg1	species					489:495	Ulva species	484:495	Ulva species	484:495	Ulva species have high economic potential since various applications were previously described including bioremediation, biofuel production, and as a source of bioactive compounds.
33338254	3	27	contain	have	497:500	arg2	potential					516:524	high economic potential	502:524	high economic potential	502:524	Ulva species have high economic potential since various applications were previously described including bioremediation, biofuel production, and as a source of bioactive compounds.
33338254	5	28	theme	Yariv	1008:1012	arg1	reagent					1014:1020	Yariv reagent	1008:1020	primary anti-AGP antibodies as well as Yariv reagent	969:1020	The reactivity with primary anti-AGP antibodies as well as Yariv reagent showed a great variety between Ulva lactuca and Solanum lycopersicum AGP-like glycoproteins.
33338254	4	29	theme	extraction	787:796	arg1	protocol					798:805	a simple extraction protocol	778:805	a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs	778:946	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	6	30	theme	amino	1125:1129	arg1	analysis					1136:1143	the amino acid analysis	1121:1143	the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent	1121:1216	While the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent showed a similarity between algal and land plant AGP-like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP-like glycoproteins.
33338254	2	31	from	plants	452:457	arg1	seaweeds					474:481	seaweeds	474:481	seaweeds	474:481	Although they are widely distributed throughout the plant kingdom and extensively studied, they remain largely unexplored in the lower plants, especially in seaweeds.
33338254	6	32	gly	glycoproteins	1161:1173	arg1	glycoproteins					1161:1173	the AGP-like glycoproteins	1148:1173	the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent	1148:1216	While the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent showed a similarity between algal and land plant AGP-like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP-like glycoproteins.
33338254	4	33	theme	Ulva	752:755	arg1	species					757:763	Ulva species	752:763	Ulva species	752:763	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	2	34	theme	plant	369:373	arg1	kingdom					375:381	the plant kingdom	365:381	the plant kingdom	365:381	Although they are widely distributed throughout the plant kingdom and extensively studied, they remain largely unexplored in the lower plants, especially in seaweeds.
33338254	4	35	gly	glycoproteins	832:844	arg1	glycoproteins					832:844	Ulva lactuca AGP-like glycoproteins	810:844	Ulva lactuca AGP-like glycoproteins	810:844	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	4	35	gly	glycoproteins	832:844	arg1	characterization					861:876	their partial characterization	847:876	their partial characterization	847:876	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	4	35	gly	glycoproteins	832:844	arg1	comparison					889:898	unique comparison	882:898	unique comparison to scarcely described Solanum lycopersicum AGPs	882:946	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	8	36	theme	AGP-like	1629:1636	arg1	glycoproteins					1638:1650	the Ulva lactuca AGP-like glycoproteins	1612:1650	the Ulva lactuca AGP-like glycoproteins	1612:1650	The exceptional structure of the Ulva lactuca AGP-like glycoproteins implies a specialized adaptation to the marine environment and might bring new insight into the evolution of the plant cell wall.
33338254	0	37	dep	Show	67:70	arg1	AGPs					112:115	AGPs	112:115	Show Unique Features Compared to Land Plants AGPs	67:115	Arabinogalactan-like Glycoproteins from Ulva lactuca (Chlorophyta) Show Unique Features Compared to Land Plants AGPs.
33338254	3	38	theme	bioactive	644:652	arg1	compounds					654:662	bioactive compounds	644:662	bioactive compounds	644:662	Ulva species have high economic potential since various applications were previously described including bioremediation, biofuel production, and as a source of bioactive compounds.
33338254	4	39	theme	glycoproteins	832:844	arg1	protocol					798:805	a simple extraction protocol	778:805	a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs	778:946	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	2	40	theme	lower	446:450	arg1	plants					452:457	the lower plants	442:457	the lower plants	442:457	Although they are widely distributed throughout the plant kingdom and extensively studied, they remain largely unexplored in the lower plants, especially in seaweeds.
33338254	8	41	theme	specialized	1662:1672	arg1	adaptation					1674:1683	a specialized adaptation	1660:1683	a specialized adaptation to the marine environment	1660:1709	The exceptional structure of the Ulva lactuca AGP-like glycoproteins implies a specialized adaptation to the marine environment and might bring new insight into the evolution of the plant cell wall.
33338254	5	42	theme	great	1031:1035	arg1	variety					1037:1043	a great variety	1029:1043	a great variety between Ulva lactuca and Solanum lycopersicum AGP-like glycoproteins	1029:1112	The reactivity with primary anti-AGP antibodies as well as Yariv reagent showed a great variety between Ulva lactuca and Solanum lycopersicum AGP-like glycoproteins.
33338254	6	43	theme	glycoproteins	1161:1173	arg1	analysis					1136:1143	the amino acid analysis	1121:1143	the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent	1121:1216	While the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent showed a similarity between algal and land plant AGP-like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP-like glycoproteins.
33338254	8	44	theme	marine	1692:1697	arg1	environment					1699:1709	the marine environment	1688:1709	the marine environment	1688:1709	The exceptional structure of the Ulva lactuca AGP-like glycoproteins implies a specialized adaptation to the marine environment and might bring new insight into the evolution of the plant cell wall.
33338254	4	45	from	confirmation	710:721	arg1	species					757:763	Ulva species	752:763	Ulva species	752:763	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	3	46	theme	Ulva	484:487	arg1	species					489:495	Ulva species	484:495	Ulva species	484:495	Ulva species have high economic potential since various applications were previously described including bioremediation, biofuel production, and as a source of bioactive compounds.
33338254	8	47	theme	plant	1765:1769	arg1	wall					1776:1779	the plant cell wall	1761:1779	the plant cell wall	1761:1779	The exceptional structure of the Ulva lactuca AGP-like glycoproteins implies a specialized adaptation to the marine environment and might bring new insight into the evolution of the plant cell wall.
33338254	8	48	theme	wall	1776:1779	arg1	evolution					1748:1756	the evolution	1744:1756	the evolution of the plant cell wall	1744:1779	The exceptional structure of the Ulva lactuca AGP-like glycoproteins implies a specialized adaptation to the marine environment and might bring new insight into the evolution of the plant cell wall.
33338254	1	49	theme	plant	242:246	arg1	development					248:258	plant development	242:258	plant development	242:258	Arabinogalactan proteins (AGPs) encompass a diverse group of plant cell wall proteoglycans, which play an essential role in plant development, signaling, plant-microbe interactions, and many others.
33338254	6	50	gly	glycoproteins	1276:1288	arg1	glycoproteins					1276:1288	plant AGP-like glycoproteins	1261:1288	plant AGP-like glycoproteins	1261:1288	While the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent showed a similarity between algal and land plant AGP-like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP-like glycoproteins.
33338254	3	51	theme	economic	507:514	arg1	potential					516:524	high economic potential	502:524	high economic potential	502:524	Ulva species have high economic potential since various applications were previously described including bioremediation, biofuel production, and as a source of bioactive compounds.
33338254	1	52	theme	diverse	162:168	arg1	proteoglycans					195:207	plant cell wall proteoglycans	179:207	plant cell wall proteoglycans	179:207	Arabinogalactan proteins (AGPs) encompass a diverse group of plant cell wall proteoglycans, which play an essential role in plant development, signaling, plant-microbe interactions, and many others.
33338254	1	52	theme	diverse	162:168	arg1	group					170:174	a diverse group	160:174	a diverse group of plant cell wall proteoglycans, which play an essential role in plant development, signaling, plant-microbe interactions, and many others	160:314	Arabinogalactan proteins (AGPs) encompass a diverse group of plant cell wall proteoglycans, which play an essential role in plant development, signaling, plant-microbe interactions, and many others.
33338254	6	53	dep	Ulva	1356:1359	arg1	lactuca					1361:1367	lactuca	1361:1367	lactuca	1361:1367	While the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent showed a similarity between algal and land plant AGP-like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP-like glycoproteins.
33338254	4	54	theme	partial	853:859	arg1	glycoproteins					832:844	Ulva lactuca AGP-like glycoproteins	810:844	Ulva lactuca AGP-like glycoproteins	810:844	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	4	54	theme	partial	853:859	arg1	characterization					861:876	their partial characterization	847:876	their partial characterization	847:876	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	5	55	dep	lactuca	1058:1064	arg1	glycoproteins					1100:1112	AGP-like glycoproteins	1091:1112	AGP-like glycoproteins	1091:1112	The reactivity with primary anti-AGP antibodies as well as Yariv reagent showed a great variety between Ulva lactuca and Solanum lycopersicum AGP-like glycoproteins.
33338254	1	56	theme	cell	185:188	arg1	proteoglycans					195:207	plant cell wall proteoglycans	179:207	plant cell wall proteoglycans	179:207	Arabinogalactan proteins (AGPs) encompass a diverse group of plant cell wall proteoglycans, which play an essential role in plant development, signaling, plant-microbe interactions, and many others.
33338254	6	57	theme	β-d-glucosyl	1191:1202	arg1	reagent					1210:1216	the β-d-glucosyl Yariv reagent	1187:1216	the β-d-glucosyl Yariv reagent	1187:1216	While the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent showed a similarity between algal and land plant AGP-like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP-like glycoproteins.
33338254	6	58	gly	glycoproteins	1378:1390	arg1	glycoproteins					1378:1390	the Ulva lactuca AGP-like glycoproteins	1352:1390	the Ulva lactuca AGP-like glycoproteins	1352:1390	While the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent showed a similarity between algal and land plant AGP-like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP-like glycoproteins.
33338254	1	59	theme	proteoglycans	195:207	arg1	proteoglycans					195:207	plant cell wall proteoglycans	179:207	plant cell wall proteoglycans	179:207	Arabinogalactan proteins (AGPs) encompass a diverse group of plant cell wall proteoglycans, which play an essential role in plant development, signaling, plant-microbe interactions, and many others.
33338254	1	59	theme	proteoglycans	195:207	arg1	group					170:174	a diverse group	160:174	a diverse group of plant cell wall proteoglycans, which play an essential role in plant development, signaling, plant-microbe interactions, and many others	160:314	Arabinogalactan proteins (AGPs) encompass a diverse group of plant cell wall proteoglycans, which play an essential role in plant development, signaling, plant-microbe interactions, and many others.
33338254	6	60	theme	neutral	1291:1297	arg1	analysis					1310:1317	neutral saccharide analysis	1291:1317	neutral saccharide analysis	1291:1317	While the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent showed a similarity between algal and land plant AGP-like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP-like glycoproteins.
33338254	8	61	theme	exceptional	1587:1597	arg1	structure					1599:1607	The exceptional structure	1583:1607	The exceptional structure of the Ulva lactuca AGP-like glycoproteins	1583:1650	The exceptional structure of the Ulva lactuca AGP-like glycoproteins implies a specialized adaptation to the marine environment and might bring new insight into the evolution of the plant cell wall.
33338254	4	62	theme	Solanum	922:928	arg1	AGPs					943:946	scarcely described Solanum lycopersicum AGPs	903:946	scarcely described Solanum lycopersicum AGPs	903:946	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	6	63	theme	glycoproteins	1378:1390	arg1	glycosylation					1335:1347	unique glycosylation	1328:1347	unique glycosylation of the Ulva lactuca AGP-like glycoproteins	1328:1390	While the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent showed a similarity between algal and land plant AGP-like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP-like glycoproteins.
33338254	5	64	theme	AGP-like	1091:1098	arg1	glycoproteins					1100:1112	AGP-like glycoproteins	1091:1112	AGP-like glycoproteins	1091:1112	The reactivity with primary anti-AGP antibodies as well as Yariv reagent showed a great variety between Ulva lactuca and Solanum lycopersicum AGP-like glycoproteins.
33338254	6	65	theme	plant	1261:1265	arg1	glycoproteins					1276:1288	plant AGP-like glycoproteins	1261:1288	plant AGP-like glycoproteins	1261:1288	While the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent showed a similarity between algal and land plant AGP-like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP-like glycoproteins.
33338254	4	66	theme	AGP-like	726:733	arg1	glycoproteins					735:747	AGP-like glycoproteins	726:747	AGP-like glycoproteins in Ulva species	726:763	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	0	67	theme	Unique	72:77	arg1	Features					79:86	Unique Features	72:86	Unique Features Compared to Land Plants	72:110	Arabinogalactan-like Glycoproteins from Ulva lactuca (Chlorophyta) Show Unique Features Compared to Land Plants AGPs.
33338254	8	68	theme	Ulva	1616:1619	arg1	glycoproteins					1638:1650	the Ulva lactuca AGP-like glycoproteins	1612:1650	the Ulva lactuca AGP-like glycoproteins	1612:1650	The exceptional structure of the Ulva lactuca AGP-like glycoproteins implies a specialized adaptation to the marine environment and might bring new insight into the evolution of the plant cell wall.
33338254	4	69	theme	first	691:695	arg1	confirmation					710:721	the first experimental confirmation	687:721	the first experimental confirmation of AGP-like glycoproteins in Ulva species	687:763	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	0	70	theme	Land	100:103	arg1	Plants					105:110	Land Plants	100:110	Land Plants	100:110	Arabinogalactan-like Glycoproteins from Ulva lactuca (Chlorophyta) Show Unique Features Compared to Land Plants AGPs.
33338254	8	71	gly	glycoproteins	1638:1650	arg1	glycoproteins					1638:1650	the Ulva lactuca AGP-like glycoproteins	1612:1650	the Ulva lactuca AGP-like glycoproteins	1612:1650	The exceptional structure of the Ulva lactuca AGP-like glycoproteins implies a specialized adaptation to the marine environment and might bring new insight into the evolution of the plant cell wall.
33338254	6	72	theme	acid	1131:1134	arg1	analysis					1136:1143	the amino acid analysis	1121:1143	the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent	1121:1216	While the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent showed a similarity between algal and land plant AGP-like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP-like glycoproteins.
33338254	4	73	theme	simple	780:785	arg1	protocol					798:805	a simple extraction protocol	778:805	a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs	778:946	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	4	74	from	species	757:763	arg1	confirmation					710:721	the first experimental confirmation	687:721	the first experimental confirmation of AGP-like glycoproteins in Ulva species	687:763	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	5	75	theme	anti-AGP	977:984	arg1	antibodies					986:995	primary anti-AGP antibodies	969:995	primary anti-AGP antibodies as well as Yariv reagent	969:1020	The reactivity with primary anti-AGP antibodies as well as Yariv reagent showed a great variety between Ulva lactuca and Solanum lycopersicum AGP-like glycoproteins.
33338254	8	76	theme	glycoproteins	1638:1650	arg1	structure					1599:1607	The exceptional structure	1583:1607	The exceptional structure of the Ulva lactuca AGP-like glycoproteins	1583:1650	The exceptional structure of the Ulva lactuca AGP-like glycoproteins implies a specialized adaptation to the marine environment and might bring new insight into the evolution of the plant cell wall.
33338254	5	77	with	reactivity	953:962	arg1	reagent					1014:1020	Yariv reagent	1008:1020	primary anti-AGP antibodies as well as Yariv reagent	969:1020	The reactivity with primary anti-AGP antibodies as well as Yariv reagent showed a great variety between Ulva lactuca and Solanum lycopersicum AGP-like glycoproteins.
33338254	5	77	with	reactivity	953:962	arg1	antibodies					986:995	primary anti-AGP antibodies	969:995	primary anti-AGP antibodies as well as Yariv reagent	969:1020	The reactivity with primary anti-AGP antibodies as well as Yariv reagent showed a great variety between Ulva lactuca and Solanum lycopersicum AGP-like glycoproteins.
33338254	1	78	theme	plant-microbe	272:284	arg1	interactions					286:297	plant-microbe interactions	272:297	plant-microbe interactions	272:297	Arabinogalactan proteins (AGPs) encompass a diverse group of plant cell wall proteoglycans, which play an essential role in plant development, signaling, plant-microbe interactions, and many others.
33338254	4	79	theme	AGP-like	823:830	arg1	glycoproteins					832:844	Ulva lactuca AGP-like glycoproteins	810:844	Ulva lactuca AGP-like glycoproteins	810:844	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	4	79	theme	AGP-like	823:830	arg1	characterization					861:876	their partial characterization	847:876	their partial characterization	847:876	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	4	79	theme	AGP-like	823:830	arg1	comparison					889:898	unique comparison	882:898	unique comparison to scarcely described Solanum lycopersicum AGPs	882:946	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	4	80	from	glycoproteins	735:747	arg1	species					757:763	Ulva species	752:763	Ulva species	752:763	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	1	81	theme	many	304:307	arg1	others					309:314	many others	304:314	many others	304:314	Arabinogalactan proteins (AGPs) encompass a diverse group of plant cell wall proteoglycans, which play an essential role in plant development, signaling, plant-microbe interactions, and many others.
33338254	3	82	theme	compounds	654:662	arg1	source					634:639	a source	632:639	a source of bioactive compounds	632:662	Ulva species have high economic potential since various applications were previously described including bioremediation, biofuel production, and as a source of bioactive compounds.
33338254	8	83	dep	Ulva	1616:1619	arg1	lactuca					1621:1627	lactuca	1621:1627	lactuca	1621:1627	The exceptional structure of the Ulva lactuca AGP-like glycoproteins implies a specialized adaptation to the marine environment and might bring new insight into the evolution of the plant cell wall.
33338254	6	84	theme	AGP-like	1152:1159	arg1	glycoproteins					1161:1173	the AGP-like glycoproteins	1148:1173	the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent	1148:1216	While the amino acid analysis of the AGP-like glycoproteins purified by the β-d-glucosyl Yariv reagent showed a similarity between algal and land plant AGP-like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP-like glycoproteins.
33338254	4	85	theme	Ulva	810:813	arg1	glycoproteins					832:844	Ulva lactuca AGP-like glycoproteins	810:844	Ulva lactuca AGP-like glycoproteins	810:844	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	4	85	theme	Ulva	810:813	arg1	characterization					861:876	their partial characterization	847:876	their partial characterization	847:876	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	4	85	theme	Ulva	810:813	arg1	comparison					889:898	unique comparison	882:898	unique comparison to scarcely described Solanum lycopersicum AGPs	882:946	This article presents the first experimental confirmation of AGP-like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP-like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs.
33338254	3	86	theme	high	502:505	arg1	potential					516:524	high economic potential	502:524	high economic potential	502:524	Ulva species have high economic potential since various applications were previously described including bioremediation, biofuel production, and as a source of bioactive compounds.
33338254	8	87	theme	cell	1771:1774	arg1	wall					1776:1779	the plant cell wall	1761:1779	the plant cell wall	1761:1779	The exceptional structure of the Ulva lactuca AGP-like glycoproteins implies a specialized adaptation to the marine environment and might bring new insight into the evolution of the plant cell wall.
33338254	1	88	theme	essential	224:232	arg1	role					234:237	an essential role	221:237	an essential role	221:237	Arabinogalactan proteins (AGPs) encompass a diverse group of plant cell wall proteoglycans, which play an essential role in plant development, signaling, plant-microbe interactions, and many others.
33910753	4	0	theme	high	965:968	arg1	TC					979:980	a high in-plane TC	963:980	a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance	963:1030	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	3	1	theme	resistance	538:547	arg1	integration					505:515	synergetic integration	494:515	synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites	494:586	Herein, synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites has been successfully achieved by the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP).
33910753	5	2	theme	new	1207:1209	arg1	opportunity					1211:1221	a new opportunity	1205:1221	a new opportunity for the thermal management of flexible electronics	1205:1272	Meantime, this eco-friendly nanocellulose-based composite also exhibits remarkable flexibility, folding endurance, and mechanical robustness, robustness, which may open up a new opportunity for the thermal management of flexible electronics.
33910753	0	3	theme	nanocellulose	82:94	arg1	composites					96:105	nacre-like nanocellulose composites	71:105	nacre-like nanocellulose composites	71:105	Synergetic integration of thermal conductivity and flame resistance in nacre-like nanocellulose composites.
33910753	0	4	from	integration	11:21	arg1	composites					96:105	nacre-like nanocellulose composites	71:105	nacre-like nanocellulose composites	71:105	Synergetic integration of thermal conductivity and flame resistance in nacre-like nanocellulose composites.
33910753	1	5	theme	great	299:303	arg1	promise					305:311	great promise	299:311	great promise in next-generation green and flexible electronics	299:361	Highly thermally conductive and flame resistant nanocellulose-based composites can synchronously achieve efficient thermal dissipation and low fire hazards of electronic devices, which shows great promise in next-generation green and flexible electronics.
33910753	5	6	theme	flexible	1253:1260	arg1	electronics					1262:1272	flexible electronics	1253:1272	flexible electronics	1253:1272	Meantime, this eco-friendly nanocellulose-based composite also exhibits remarkable flexibility, folding endurance, and mechanical robustness, robustness, which may open up a new opportunity for the thermal management of flexible electronics.
33910753	4	7	from	immobilization	859:872	arg1	Benefiting					742:751	Benefiting	742:751	Benefiting	742:751	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	4	8	theme	successful	848:857	arg1	APP					901:903	APP	901:903	APP	901:903	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	4	8	theme	successful	848:857	arg1	immobilization					859:872	successful immobilization	848:872	successful immobilization of ammonium polyphosphate (APP)	848:904	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	5	9	theme	nanocellulose-based	1061:1079	arg1	composite					1081:1089	this eco-friendly nanocellulose-based composite	1043:1089	this eco-friendly nanocellulose-based composite	1043:1089	Meantime, this eco-friendly nanocellulose-based composite also exhibits remarkable flexibility, folding endurance, and mechanical robustness, robustness, which may open up a new opportunity for the thermal management of flexible electronics.
33910753	5	9	theme	nanocellulose-based	1061:1079	arg1	Meantime					1033:1040	Meantime	1033:1040	Meantime	1033:1040	Meantime, this eco-friendly nanocellulose-based composite also exhibits remarkable flexibility, folding endurance, and mechanical robustness, robustness, which may open up a new opportunity for the thermal management of flexible electronics.
33910753	0	10	from	resistance	57:66	arg1	composites					96:105	nacre-like nanocellulose composites	71:105	nacre-like nanocellulose composites	71:105	Synergetic integration of thermal conductivity and flame resistance in nacre-like nanocellulose composites.
33910753	4	11	theme	in-plane	970:977	arg1	TC					979:980	a high in-plane TC	963:980	a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance	963:1030	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	3	12	theme	TC	525:526	arg1	integration					505:515	synergetic integration	494:515	synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites	494:586	Herein, synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites has been successfully achieved by the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP).
33910753	4	13	theme	composite	939:947	arg1	film					949:952	the as-obtained CNFs/BNNS-p-APP composite film	907:952	the as-obtained CNFs/BNNS-p-APP composite film	907:952	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	1	14	theme	efficient	213:221	arg1	dissipation					231:241	efficient thermal dissipation	213:241	efficient thermal dissipation	213:241	Highly thermally conductive and flame resistant nanocellulose-based composites can synchronously achieve efficient thermal dissipation and low fire hazards of electronic devices, which shows great promise in next-generation green and flexible electronics.
33910753	4	15	theme	CNFs/BNNS-p-APP	923:937	arg1	film					949:952	the as-obtained CNFs/BNNS-p-APP composite film	907:952	the as-obtained CNFs/BNNS-p-APP composite film	907:952	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	5	16	theme	remarkable	1105:1114	arg1	flexibility					1116:1126	remarkable flexibility	1105:1126	remarkable flexibility	1105:1126	Meantime, this eco-friendly nanocellulose-based composite also exhibits remarkable flexibility, folding endurance, and mechanical robustness, robustness, which may open up a new opportunity for the thermal management of flexible electronics.
33910753	1	17	theme	thermal	223:229	arg1	dissipation					231:241	efficient thermal dissipation	213:241	efficient thermal dissipation	213:241	Highly thermally conductive and flame resistant nanocellulose-based composites can synchronously achieve efficient thermal dissipation and low fire hazards of electronic devices, which shows great promise in next-generation green and flexible electronics.
33910753	4	18	from	interaction	831:841	arg1	Benefiting					742:751	Benefiting	742:751	Benefiting	742:751	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	4	19	theme	hydrogen-bonding	814:829	arg1	interaction					831:841	strong hydrogen-bonding interaction	807:841	strong hydrogen-bonding interaction	807:841	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	2	20	theme	thermal	423:429	arg1	conductivity					431:442	the high thermal conductivity	414:442	the high thermal conductivity (TC)	414:447	However, it has long been intractable to optimize the high thermal conductivity (TC) and flame resistance simultaneously.
33910753	2	20	theme	thermal	423:429	arg1	TC					445:446	TC	445:446	TC	445:446	However, it has long been intractable to optimize the high thermal conductivity (TC) and flame resistance simultaneously.
33910753	0	21	theme	Synergetic	0:9	arg1	integration					11:21	Synergetic integration	0:21	Synergetic integration of thermal conductivity	0:45	Synergetic integration of thermal conductivity and flame resistance in nacre-like nanocellulose composites.
33910753	1	22	theme	next-generation	316:330	arg1	green					332:336	next-generation green	316:336	next-generation green	316:336	Highly thermally conductive and flame resistant nanocellulose-based composites can synchronously achieve efficient thermal dissipation and low fire hazards of electronic devices, which shows great promise in next-generation green and flexible electronics.
33910753	3	23	theme	vacuum-assisted	626:640	arg1	filtration					642:651	the vacuum-assisted filtration	622:651	the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP)	622:739	Herein, synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites has been successfully achieved by the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP).
33910753	2	24	theme	high	418:421	arg1	conductivity					431:442	the high thermal conductivity	414:442	the high thermal conductivity (TC)	414:447	However, it has long been intractable to optimize the high thermal conductivity (TC) and flame resistance simultaneously.
33910753	2	24	theme	high	418:421	arg1	TC					445:446	TC	445:446	TC	445:446	However, it has long been intractable to optimize the high thermal conductivity (TC) and flame resistance simultaneously.
33910753	0	25	theme	thermal	26:32	arg1	conductivity					34:45	thermal conductivity	26:45	thermal conductivity	26:45	Synergetic integration of thermal conductivity and flame resistance in nacre-like nanocellulose composites.
33910753	1	26	from	promise	305:311	arg1	green					332:336	next-generation green	316:336	next-generation green	316:336	Highly thermally conductive and flame resistant nanocellulose-based composites can synchronously achieve efficient thermal dissipation and low fire hazards of electronic devices, which shows great promise in next-generation green and flexible electronics.
33910753	1	26	from	promise	305:311	arg1	electronics					351:361	flexible electronics	342:361	flexible electronics	342:361	Highly thermally conductive and flame resistant nanocellulose-based composites can synchronously achieve efficient thermal dissipation and low fire hazards of electronic devices, which shows great promise in next-generation green and flexible electronics.
33910753	5	27	theme	eco-friendly	1048:1059	arg1	composite					1081:1089	this eco-friendly nanocellulose-based composite	1043:1089	this eco-friendly nanocellulose-based composite	1043:1089	Meantime, this eco-friendly nanocellulose-based composite also exhibits remarkable flexibility, folding endurance, and mechanical robustness, robustness, which may open up a new opportunity for the thermal management of flexible electronics.
33910753	5	27	theme	eco-friendly	1048:1059	arg1	Meantime					1033:1040	Meantime	1033:1040	Meantime	1033:1040	Meantime, this eco-friendly nanocellulose-based composite also exhibits remarkable flexibility, folding endurance, and mechanical robustness, robustness, which may open up a new opportunity for the thermal management of flexible electronics.
33910753	2	28	theme	flame	453:457	arg1	resistance					459:468	flame resistance	453:468	flame resistance	453:468	However, it has long been intractable to optimize the high thermal conductivity (TC) and flame resistance simultaneously.
33910753	4	29	theme	strong	807:812	arg1	interaction					831:841	strong hydrogen-bonding interaction	807:841	strong hydrogen-bonding interaction	807:841	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	4	30	theme	flame	1015:1019	arg1	resistance					1021:1030	outstanding flame resistance	1003:1030	outstanding flame resistance	1003:1030	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	3	31	theme	nitride	709:715	arg1	BNNS-p-APP					729:738	BNNS-p-APP	729:738	BNNS-p-APP	729:738	Herein, synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites has been successfully achieved by the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP).
33910753	3	31	theme	nitride	709:715	arg1	nanosheets					717:726	functionalized boron nitride nanosheets	688:726	functionalized boron nitride nanosheets (BNNS-p-APP)	688:739	Herein, synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites has been successfully achieved by the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP).
33910753	1	32	theme	conductive	125:134	arg1	composites					176:185	Highly thermally conductive and flame resistant nanocellulose-based composites	108:185	Highly thermally conductive and flame resistant nanocellulose-based composites	108:185	Highly thermally conductive and flame resistant nanocellulose-based composites can synchronously achieve efficient thermal dissipation and low fire hazards of electronic devices, which shows great promise in next-generation green and flexible electronics.
33910753	5	33	theme	mechanical	1152:1161	arg1	robustness					1163:1172	mechanical robustness	1152:1172	mechanical robustness	1152:1172	Meantime, this eco-friendly nanocellulose-based composite also exhibits remarkable flexibility, folding endurance, and mechanical robustness, robustness, which may open up a new opportunity for the thermal management of flexible electronics.
33910753	1	34	theme	fire	251:254	arg1	hazards					256:262	low fire hazards	247:262	low fire hazards	247:262	Highly thermally conductive and flame resistant nanocellulose-based composites can synchronously achieve efficient thermal dissipation and low fire hazards of electronic devices, which shows great promise in next-generation green and flexible electronics.
33910753	1	35	theme	flexible	342:349	arg1	electronics					351:361	flexible electronics	342:361	flexible electronics	342:361	Highly thermally conductive and flame resistant nanocellulose-based composites can synchronously achieve efficient thermal dissipation and low fire hazards of electronic devices, which shows great promise in next-generation green and flexible electronics.
33910753	3	36	theme	nanosheets	717:726	arg1	filtration					642:651	the vacuum-assisted filtration	622:651	the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP)	622:739	Herein, synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites has been successfully achieved by the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP).
33910753	3	37	theme	cellulose	656:664	arg1	CNFs					678:681	CNFs	678:681	CNFs	678:681	Herein, synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites has been successfully achieved by the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP).
33910753	3	37	theme	cellulose	656:664	arg1	nanofibers					666:675	cellulose nanofibers	656:675	cellulose nanofibers (CNFs)	656:682	Herein, synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites has been successfully achieved by the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP).
33910753	0	38	theme	conductivity	34:45	arg1	integration					11:21	Synergetic integration	0:21	Synergetic integration of thermal conductivity	0:45	Synergetic integration of thermal conductivity and flame resistance in nacre-like nanocellulose composites.
33910753	0	38	theme	conductivity	34:45	arg1	resistance					57:66	flame resistance	51:66	flame resistance in nacre-like nanocellulose composites	51:105	Synergetic integration of thermal conductivity and flame resistance in nacre-like nanocellulose composites.
33910753	5	39	theme	electronics	1262:1272	arg1	management					1239:1248	the thermal management	1227:1248	the thermal management of flexible electronics	1227:1272	Meantime, this eco-friendly nanocellulose-based composite also exhibits remarkable flexibility, folding endurance, and mechanical robustness, robustness, which may open up a new opportunity for the thermal management of flexible electronics.
33910753	3	40	theme	functionalized	688:701	arg1	BNNS-p-APP					729:738	BNNS-p-APP	729:738	BNNS-p-APP	729:738	Herein, synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites has been successfully achieved by the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP).
33910753	3	40	theme	functionalized	688:701	arg1	nanosheets					717:726	functionalized boron nitride nanosheets	688:726	functionalized boron nitride nanosheets (BNNS-p-APP)	688:739	Herein, synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites has been successfully achieved by the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP).
33910753	3	41	theme	nanofibers	666:675	arg1	filtration					642:651	the vacuum-assisted filtration	622:651	the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP)	622:739	Herein, synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites has been successfully achieved by the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP).
33910753	3	42	theme	synergetic	494:503	arg1	integration					505:515	synergetic integration	494:515	synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites	494:586	Herein, synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites has been successfully achieved by the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP).
33910753	4	43	theme	9.1 W m-1 K-1	985:997	arg1	TC					979:980	a high in-plane TC	963:980	a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance	963:1030	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	3	44	theme	boron	703:707	arg1	BNNS-p-APP					729:738	BNNS-p-APP	729:738	BNNS-p-APP	729:738	Herein, synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites has been successfully achieved by the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP).
33910753	3	44	theme	boron	703:707	arg1	nanosheets					717:726	functionalized boron nitride nanosheets	688:726	functionalized boron nitride nanosheets (BNNS-p-APP)	688:739	Herein, synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites has been successfully achieved by the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP).
33910753	4	45	theme	as-obtained	911:921	arg1	film					949:952	the as-obtained CNFs/BNNS-p-APP composite film	907:952	the as-obtained CNFs/BNNS-p-APP composite film	907:952	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	4	46	theme	hierarchical	778:789	arg1	microstructure					791:804	the highly oriented hierarchical microstructure	758:804	the highly oriented hierarchical microstructure	758:804	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	0	47	theme	flame	51:55	arg1	resistance					57:66	flame resistance	51:66	flame resistance in nacre-like nanocellulose composites	51:105	Synergetic integration of thermal conductivity and flame resistance in nacre-like nanocellulose composites.
33910753	3	48	theme	high	520:523	arg1	TC					525:526	high TC	520:526	high TC	520:526	Herein, synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites has been successfully achieved by the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP).
33910753	3	49	from	integration	505:515	arg1	composites					577:586	nacre-like nanocellulose composites	552:586	nacre-like nanocellulose composites	552:586	Herein, synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites has been successfully achieved by the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP).
33910753	3	50	theme	flame	532:536	arg1	resistance					538:547	flame resistance	532:547	flame resistance	532:547	Herein, synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites has been successfully achieved by the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP).
33910753	4	51	theme	outstanding	1003:1013	arg1	resistance					1021:1030	outstanding flame resistance	1003:1030	outstanding flame resistance	1003:1030	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	4	52	theme	polyphosphate	886:898	arg1	interaction					831:841	strong hydrogen-bonding interaction	807:841	strong hydrogen-bonding interaction	807:841	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	4	52	theme	polyphosphate	886:898	arg1	immobilization					859:872	successful immobilization	848:872	successful immobilization of ammonium polyphosphate (APP)	848:904	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	4	52	theme	polyphosphate	886:898	arg1	APP					901:903	APP	901:903	APP	901:903	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	4	52	theme	polyphosphate	886:898	arg1	microstructure					791:804	the highly oriented hierarchical microstructure	758:804	the highly oriented hierarchical microstructure	758:804	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	1	53	theme	resistant	146:154	arg1	composites					176:185	Highly thermally conductive and flame resistant nanocellulose-based composites	108:185	Highly thermally conductive and flame resistant nanocellulose-based composites	108:185	Highly thermally conductive and flame resistant nanocellulose-based composites can synchronously achieve efficient thermal dissipation and low fire hazards of electronic devices, which shows great promise in next-generation green and flexible electronics.
33910753	4	54	from	microstructure	791:804	arg1	Benefiting					742:751	Benefiting	742:751	Benefiting	742:751	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	1	55	theme	electronic	267:276	arg1	devices					278:284	electronic devices	267:284	electronic devices	267:284	Highly thermally conductive and flame resistant nanocellulose-based composites can synchronously achieve efficient thermal dissipation and low fire hazards of electronic devices, which shows great promise in next-generation green and flexible electronics.
33910753	4	56	theme	ammonium	877:884	arg1	polyphosphate					886:898	ammonium polyphosphate	877:898	ammonium polyphosphate	877:898	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	1	57	theme	nanocellulose-based	156:174	arg1	composites					176:185	Highly thermally conductive and flame resistant nanocellulose-based composites	108:185	Highly thermally conductive and flame resistant nanocellulose-based composites	108:185	Highly thermally conductive and flame resistant nanocellulose-based composites can synchronously achieve efficient thermal dissipation and low fire hazards of electronic devices, which shows great promise in next-generation green and flexible electronics.
33910753	1	58	theme	devices	278:284	arg1	dissipation					231:241	efficient thermal dissipation	213:241	efficient thermal dissipation	213:241	Highly thermally conductive and flame resistant nanocellulose-based composites can synchronously achieve efficient thermal dissipation and low fire hazards of electronic devices, which shows great promise in next-generation green and flexible electronics.
33910753	1	58	theme	devices	278:284	arg1	hazards					256:262	low fire hazards	247:262	low fire hazards	247:262	Highly thermally conductive and flame resistant nanocellulose-based composites can synchronously achieve efficient thermal dissipation and low fire hazards of electronic devices, which shows great promise in next-generation green and flexible electronics.
33910753	0	59	theme	nacre-like	71:80	arg1	composites					96:105	nacre-like nanocellulose composites	71:105	nacre-like nanocellulose composites	71:105	Synergetic integration of thermal conductivity and flame resistance in nacre-like nanocellulose composites.
33910753	4	60	theme	resistance	1021:1030	arg1	TC					979:980	a high in-plane TC	963:980	a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance	963:1030	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33910753	5	61	theme	folding	1129:1135	arg1	endurance					1137:1145	folding endurance	1129:1145	folding endurance	1129:1145	Meantime, this eco-friendly nanocellulose-based composite also exhibits remarkable flexibility, folding endurance, and mechanical robustness, robustness, which may open up a new opportunity for the thermal management of flexible electronics.
33910753	1	62	theme	low	247:249	arg1	hazards					256:262	low fire hazards	247:262	low fire hazards	247:262	Highly thermally conductive and flame resistant nanocellulose-based composites can synchronously achieve efficient thermal dissipation and low fire hazards of electronic devices, which shows great promise in next-generation green and flexible electronics.
33910753	3	63	theme	nacre-like	552:561	arg1	composites					577:586	nacre-like nanocellulose composites	552:586	nacre-like nanocellulose composites	552:586	Herein, synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites has been successfully achieved by the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP).
33910753	5	64	theme	thermal	1231:1237	arg1	management					1239:1248	the thermal management	1227:1248	the thermal management of flexible electronics	1227:1272	Meantime, this eco-friendly nanocellulose-based composite also exhibits remarkable flexibility, folding endurance, and mechanical robustness, robustness, which may open up a new opportunity for the thermal management of flexible electronics.
33910753	3	65	theme	nanocellulose	563:575	arg1	composites					577:586	nacre-like nanocellulose composites	552:586	nacre-like nanocellulose composites	552:586	Herein, synergetic integration of high TC and flame resistance in nacre-like nanocellulose composites has been successfully achieved by the vacuum-assisted filtration of cellulose nanofibers (CNFs) and functionalized boron nitride nanosheets (BNNS-p-APP).
33910753	4	66	theme	oriented	769:776	arg1	microstructure					791:804	the highly oriented hierarchical microstructure	758:804	the highly oriented hierarchical microstructure	758:804	Benefiting from the highly oriented hierarchical microstructure, strong hydrogen-bonding interaction, and successful immobilization of ammonium polyphosphate (APP), the as-obtained CNFs/BNNS-p-APP composite film achieves a high in-plane TC of 9.1 W m-1 K-1 and outstanding flame resistance.
33019826	6	0	theme	hypoglycemic	1148:1159	arg1	effects					1161:1167	comparable hypoglycemic effects	1137:1167	comparable hypoglycemic effects to free insulin	1137:1183	For instance, subcutaneous injection of nanoparticles comprised of chitosan combined with sodium tripolyphosphate, Pluronic or alginate/calcium chloride, resulted in comparable hypoglycemic effects to free insulin, at two-fold lower dose.
33019826	8	1	theme	natural/synthetic	1526:1542	arg1	polymers					1544:1551	several natural/synthetic polymers	1518:1551	several natural/synthetic polymers	1518:1551	This study indicates that characteristics and delivery efficiency of nanomaterials can be controlled via utilizing several natural/synthetic polymers and by fine-tuning of combination ratio between polymers.
33019826	5	2	theme	oral	798:801	arg1	administration					803:816	subcutaneous and oral administration	781:816	administration	803:816	After subcutaneous and oral administration in diabetic rats, some of the insulin-loaded nanoparticles were able to induce much higher hypoglycemic effect as compared to the unloaded free insulin.
33019826	5	3	from	administration	803:816	arg1	rats					830:833	diabetic rats	821:833	diabetic rats	821:833	After subcutaneous and oral administration in diabetic rats, some of the insulin-loaded nanoparticles were able to induce much higher hypoglycemic effect as compared to the unloaded free insulin.
33019826	2	4	theme	natural	369:375	arg1	polymers					391:398	natural and synthetic polymers	369:398	natural and synthetic polymers	369:398	Several combinations of natural and synthetic polymers have been utilized for preparation of nanoparticles including, chitosan, alginate, albumin and Pluronic.
33019826	1	5	theme	blood	258:262	arg1	levels					272:277	blood glucose levels	258:277	blood glucose levels in diabetic rats following subcutaneous and oral administrations	258:342	The aims of the current study were to develop insulin-loaded nanoparticles comprised of various polymers at different compositions, and to evaluate their ability to lower blood glucose levels in diabetic rats following subcutaneous and oral administrations.
33019826	8	6	theme	delivery	1449:1456	arg1	efficiency					1458:1467	delivery efficiency	1449:1467	delivery efficiency	1449:1467	This study indicates that characteristics and delivery efficiency of nanomaterials can be controlled via utilizing several natural/synthetic polymers and by fine-tuning of combination ratio between polymers.
33019826	3	7	theme	efficiency	573:582	arg1	170 nm-800 nm					516:528	170 nm-800 nm	516:528	170 nm-800 nm	516:528	Nanosized (170 nm-800 nm) spherical particles of high encapsulation efficiency (15-52%) have been prepared.
33019826	3	7	theme	efficiency	573:582	arg1	particles					541:549	Nanosized (170 nm-800 nm) spherical particles	505:549	Nanosized (170 nm-800 nm) spherical particles of high encapsulation efficiency (15-52%)	505:591	Nanosized (170 nm-800 nm) spherical particles of high encapsulation efficiency (15-52%) have been prepared.
33019826	4	8	theme	particles	764:772	arg1	size					705:708	size	705:708	size	705:708	Composition and ratios between the integrated polymers played a pivotal role in determining size, zeta potential, and in vivo hypoglycemic activity of particles.
33019826	4	8	theme	particles	764:772	arg1	potential					716:724	zeta potential	711:724	zeta potential	711:724	Composition and ratios between the integrated polymers played a pivotal role in determining size, zeta potential, and in vivo hypoglycemic activity of particles.
33019826	4	8	theme	particles	764:772	arg1	activity					752:759	in vivo hypoglycemic activity	731:759	in vivo hypoglycemic activity	731:759	Composition and ratios between the integrated polymers played a pivotal role in determining size, zeta potential, and in vivo hypoglycemic activity of particles.
33019826	1	9	theme	glucose	264:270	arg1	levels					272:277	blood glucose levels	258:277	blood glucose levels in diabetic rats following subcutaneous and oral administrations	258:342	The aims of the current study were to develop insulin-loaded nanoparticles comprised of various polymers at different compositions, and to evaluate their ability to lower blood glucose levels in diabetic rats following subcutaneous and oral administrations.
33019826	7	10	theme	alanine	1318:1324	arg1	albumin					1373:1379	albumin	1373:1379	albumin	1373:1379	Nanoparticles were well-tolerated after oral administration in rats, as evidenced by by measuring levels of alanine aminotransferase, aspartate aminotransferases, albumin, creatinine and urea.
33019826	7	10	theme	alanine	1318:1324	arg1	aminotransferase					1326:1341	alanine aminotransferase	1318:1341	alanine aminotransferase	1318:1341	Nanoparticles were well-tolerated after oral administration in rats, as evidenced by by measuring levels of alanine aminotransferase, aspartate aminotransferases, albumin, creatinine and urea.
33019826	7	10	theme	alanine	1318:1324	arg1	aminotransferases					1354:1370	aspartate aminotransferases	1344:1370	aspartate aminotransferases	1344:1370	Nanoparticles were well-tolerated after oral administration in rats, as evidenced by by measuring levels of alanine aminotransferase, aspartate aminotransferases, albumin, creatinine and urea.
33019826	4	11	theme	in vivo	731:737	arg1	activity					752:759	in vivo hypoglycemic activity	731:759	in vivo hypoglycemic activity	731:759	Composition and ratios between the integrated polymers played a pivotal role in determining size, zeta potential, and in vivo hypoglycemic activity of particles.
33019826	4	12	theme	zeta	711:714	arg1	potential					716:724	zeta potential	711:724	zeta potential	711:724	Composition and ratios between the integrated polymers played a pivotal role in determining size, zeta potential, and in vivo hypoglycemic activity of particles.
33019826	4	13	theme	integrated	648:657	arg1	polymers					659:666	the integrated polymers	644:666	the integrated polymers	644:666	Composition and ratios between the integrated polymers played a pivotal role in determining size, zeta potential, and in vivo hypoglycemic activity of particles.
33019826	3	14	theme	high	554:557	arg1	efficiency					573:582	high encapsulation efficiency	554:582	high encapsulation efficiency (15-52%)	554:591	Nanosized (170 nm-800 nm) spherical particles of high encapsulation efficiency (15-52%) have been prepared.
33019826	3	14	theme	high	554:557	arg1	%					590:590	15-52%	585:590	15-52%	585:590	Nanosized (170 nm-800 nm) spherical particles of high encapsulation efficiency (15-52%) have been prepared.
33019826	5	15	theme	unloaded	948:955	arg1	insulin					962:968	the unloaded free insulin	944:968	the unloaded free insulin	944:968	After subcutaneous and oral administration in diabetic rats, some of the insulin-loaded nanoparticles were able to induce much higher hypoglycemic effect as compared to the unloaded free insulin.
33019826	1	16	theme	various	175:181	arg1	polymers					183:190	various polymers	175:190	various polymers at different compositions	175:216	The aims of the current study were to develop insulin-loaded nanoparticles comprised of various polymers at different compositions, and to evaluate their ability to lower blood glucose levels in diabetic rats following subcutaneous and oral administrations.
33019826	6	17	theme	nanoparticles	1011:1023	arg1	injection					998:1006	subcutaneous injection	985:1006	subcutaneous injection	985:1006	For instance, subcutaneous injection of nanoparticles comprised of chitosan combined with sodium tripolyphosphate, Pluronic or alginate/calcium chloride, resulted in comparable hypoglycemic effects to free insulin, at two-fold lower dose.
33019826	6	18	dep	tripolyphosphate	1068:1083	arg1	Pluronic					1086:1093	Pluronic	1086:1093	Pluronic	1086:1093	For instance, subcutaneous injection of nanoparticles comprised of chitosan combined with sodium tripolyphosphate, Pluronic or alginate/calcium chloride, resulted in comparable hypoglycemic effects to free insulin, at two-fold lower dose.
33019826	3	19	theme	encapsulation	559:571	arg1	efficiency					573:582	high encapsulation efficiency	554:582	high encapsulation efficiency (15-52%)	554:591	Nanosized (170 nm-800 nm) spherical particles of high encapsulation efficiency (15-52%) have been prepared.
33019826	3	19	theme	encapsulation	559:571	arg1	%					590:590	15-52%	585:590	15-52%	585:590	Nanosized (170 nm-800 nm) spherical particles of high encapsulation efficiency (15-52%) have been prepared.
33019826	6	20	theme	comparable	1137:1146	arg1	effects					1161:1167	comparable hypoglycemic effects	1137:1167	comparable hypoglycemic effects to free insulin	1137:1183	For instance, subcutaneous injection of nanoparticles comprised of chitosan combined with sodium tripolyphosphate, Pluronic or alginate/calcium chloride, resulted in comparable hypoglycemic effects to free insulin, at two-fold lower dose.
33019826	2	21	theme	polymers	391:398	arg1	combinations					353:364	Several combinations	345:364	Several combinations of natural and synthetic polymers	345:398	Several combinations of natural and synthetic polymers have been utilized for preparation of nanoparticles including, chitosan, alginate, albumin and Pluronic.
33019826	7	22	theme	aminotransferase	1326:1341	arg1	levels					1308:1313	levels	1308:1313	levels of alanine aminotransferase, aspartate aminotransferases, albumin	1308:1379	Nanoparticles were well-tolerated after oral administration in rats, as evidenced by by measuring levels of alanine aminotransferase, aspartate aminotransferases, albumin, creatinine and urea.
33019826	7	22	theme	aminotransferase	1326:1341	arg1	urea					1397:1400	urea	1397:1400	urea	1397:1400	Nanoparticles were well-tolerated after oral administration in rats, as evidenced by by measuring levels of alanine aminotransferase, aspartate aminotransferases, albumin, creatinine and urea.
33019826	7	22	theme	aminotransferase	1326:1341	arg1	creatinine					1382:1391	creatinine	1382:1391	creatinine	1382:1391	Nanoparticles were well-tolerated after oral administration in rats, as evidenced by by measuring levels of alanine aminotransferase, aspartate aminotransferases, albumin, creatinine and urea.
33019826	1	23	theme	different	195:203	arg1	compositions					205:216	different compositions	195:216	different compositions	195:216	The aims of the current study were to develop insulin-loaded nanoparticles comprised of various polymers at different compositions, and to evaluate their ability to lower blood glucose levels in diabetic rats following subcutaneous and oral administrations.
33019826	1	24	theme	diabetic	282:289	arg1	rats					291:294	diabetic rats	282:294	diabetic rats	282:294	The aims of the current study were to develop insulin-loaded nanoparticles comprised of various polymers at different compositions, and to evaluate their ability to lower blood glucose levels in diabetic rats following subcutaneous and oral administrations.
33019826	5	25	theme	free	957:960	arg1	insulin					962:968	the unloaded free insulin	944:968	the unloaded free insulin	944:968	After subcutaneous and oral administration in diabetic rats, some of the insulin-loaded nanoparticles were able to induce much higher hypoglycemic effect as compared to the unloaded free insulin.
33019826	5	26	theme	diabetic	821:828	arg1	rats					830:833	diabetic rats	821:833	diabetic rats	821:833	After subcutaneous and oral administration in diabetic rats, some of the insulin-loaded nanoparticles were able to induce much higher hypoglycemic effect as compared to the unloaded free insulin.
33019826	5	27	theme	insulin-loaded	848:861	arg1	nanoparticles					863:875	the insulin-loaded nanoparticles	844:875	the insulin-loaded nanoparticles	844:875	After subcutaneous and oral administration in diabetic rats, some of the insulin-loaded nanoparticles were able to induce much higher hypoglycemic effect as compared to the unloaded free insulin.
33019826	0	28	theme	natural	26:32	arg1	polymers					48:55	natural and synthetic polymers	26:55	natural and synthetic polymers	26:55	Nanoparticles integrating natural and synthetic polymers for in vivo insulin delivery.
33019826	6	29	theme	subcutaneous	985:996	arg1	injection					998:1006	subcutaneous injection	985:1006	subcutaneous injection	985:1006	For instance, subcutaneous injection of nanoparticles comprised of chitosan combined with sodium tripolyphosphate, Pluronic or alginate/calcium chloride, resulted in comparable hypoglycemic effects to free insulin, at two-fold lower dose.
33019826	1	30	from	levels	272:277	arg1	rats					291:294	diabetic rats	282:294	diabetic rats	282:294	The aims of the current study were to develop insulin-loaded nanoparticles comprised of various polymers at different compositions, and to evaluate their ability to lower blood glucose levels in diabetic rats following subcutaneous and oral administrations.
33019826	1	31	theme	current	103:109	arg1	study					111:115	the current study	99:115	the current study	99:115	The aims of the current study were to develop insulin-loaded nanoparticles comprised of various polymers at different compositions, and to evaluate their ability to lower blood glucose levels in diabetic rats following subcutaneous and oral administrations.
33019826	2	32	theme	nanoparticles	438:450	arg1	albumin					483:489	albumin	483:489	albumin	483:489	Several combinations of natural and synthetic polymers have been utilized for preparation of nanoparticles including, chitosan, alginate, albumin and Pluronic.
33019826	2	32	theme	nanoparticles	438:450	arg1	alginate					473:480	alginate	473:480	alginate	473:480	Several combinations of natural and synthetic polymers have been utilized for preparation of nanoparticles including, chitosan, alginate, albumin and Pluronic.
33019826	2	32	theme	nanoparticles	438:450	arg1	chitosan					463:470	chitosan	463:470	chitosan	463:470	Several combinations of natural and synthetic polymers have been utilized for preparation of nanoparticles including, chitosan, alginate, albumin and Pluronic.
33019826	2	32	theme	nanoparticles	438:450	arg1	preparation					423:433	preparation	423:433	preparation of nanoparticles including	423:460	Several combinations of natural and synthetic polymers have been utilized for preparation of nanoparticles including, chitosan, alginate, albumin and Pluronic.
33019826	7	33	dep	evidenced	1282:1290	arg1	measuring					1298:1306	measuring	1298:1306	measuring levels of alanine aminotransferase, aspartate aminotransferases, albumin, creatinine and urea	1298:1400	Nanoparticles were well-tolerated after oral administration in rats, as evidenced by by measuring levels of alanine aminotransferase, aspartate aminotransferases, albumin, creatinine and urea.
33019826	7	34	from	administration	1255:1268	arg1	rats					1273:1276	rats	1273:1276	rats	1273:1276	Nanoparticles were well-tolerated after oral administration in rats, as evidenced by by measuring levels of alanine aminotransferase, aspartate aminotransferases, albumin, creatinine and urea.
33019826	8	35	theme	nanomaterials	1472:1484	arg1	characteristics					1429:1443	characteristics	1429:1443	characteristics	1429:1443	This study indicates that characteristics and delivery efficiency of nanomaterials can be controlled via utilizing several natural/synthetic polymers and by fine-tuning of combination ratio between polymers.
33019826	8	35	theme	nanomaterials	1472:1484	arg1	efficiency					1458:1467	delivery efficiency	1449:1467	delivery efficiency	1449:1467	This study indicates that characteristics and delivery efficiency of nanomaterials can be controlled via utilizing several natural/synthetic polymers and by fine-tuning of combination ratio between polymers.
33019826	5	36	theme	higher	902:907	arg1	effect					922:927	much higher hypoglycemic effect	897:927	much higher hypoglycemic effect	897:927	After subcutaneous and oral administration in diabetic rats, some of the insulin-loaded nanoparticles were able to induce much higher hypoglycemic effect as compared to the unloaded free insulin.
33019826	0	37	theme	synthetic	38:46	arg1	polymers					48:55	natural and synthetic polymers	26:55	natural and synthetic polymers	26:55	Nanoparticles integrating natural and synthetic polymers for in vivo insulin delivery.
33019826	1	38	theme	subcutaneous	306:317	arg1	administrations					328:342	subcutaneous and oral administrations	306:342	subcutaneous and oral administrations	306:342	The aims of the current study were to develop insulin-loaded nanoparticles comprised of various polymers at different compositions, and to evaluate their ability to lower blood glucose levels in diabetic rats following subcutaneous and oral administrations.
33019826	0	39	theme	in vivo	61:67	arg1	delivery					77:84	in vivo insulin delivery	61:84	in vivo insulin delivery	61:84	Nanoparticles integrating natural and synthetic polymers for in vivo insulin delivery.
33019826	5	40	theme	hypoglycemic	909:920	arg1	effect					922:927	much higher hypoglycemic effect	897:927	much higher hypoglycemic effect	897:927	After subcutaneous and oral administration in diabetic rats, some of the insulin-loaded nanoparticles were able to induce much higher hypoglycemic effect as compared to the unloaded free insulin.
33019826	5	41	theme	subcutaneous	781:792	arg1	administration					803:816	subcutaneous and oral administration	781:816	administration	803:816	After subcutaneous and oral administration in diabetic rats, some of the insulin-loaded nanoparticles were able to induce much higher hypoglycemic effect as compared to the unloaded free insulin.
33019826	1	42	theme	study	111:115	arg1	aims					91:94	The aims	87:94	The aims of the current study	87:115	The aims of the current study were to develop insulin-loaded nanoparticles comprised of various polymers at different compositions, and to evaluate their ability to lower blood glucose levels in diabetic rats following subcutaneous and oral administrations.
33019826	1	43	theme	oral	323:326	arg1	administrations					328:342	subcutaneous and oral administrations	306:342	subcutaneous and oral administrations	306:342	The aims of the current study were to develop insulin-loaded nanoparticles comprised of various polymers at different compositions, and to evaluate their ability to lower blood glucose levels in diabetic rats following subcutaneous and oral administrations.
33019826	6	44	theme	lower	1198:1202	arg1	dose					1204:1207	two-fold lower dose	1189:1207	two-fold lower dose	1189:1207	For instance, subcutaneous injection of nanoparticles comprised of chitosan combined with sodium tripolyphosphate, Pluronic or alginate/calcium chloride, resulted in comparable hypoglycemic effects to free insulin, at two-fold lower dose.
33019826	1	45	from	compositions	205:216	arg1	polymers					183:190	various polymers	175:190	various polymers at different compositions	175:216	The aims of the current study were to develop insulin-loaded nanoparticles comprised of various polymers at different compositions, and to evaluate their ability to lower blood glucose levels in diabetic rats following subcutaneous and oral administrations.
33019826	7	46	theme	aspartate	1344:1352	arg1	aminotransferase					1326:1341	alanine aminotransferase	1318:1341	alanine aminotransferase	1318:1341	Nanoparticles were well-tolerated after oral administration in rats, as evidenced by by measuring levels of alanine aminotransferase, aspartate aminotransferases, albumin, creatinine and urea.
33019826	7	46	theme	aspartate	1344:1352	arg1	aminotransferases					1354:1370	aspartate aminotransferases	1344:1370	aspartate aminotransferases	1344:1370	Nanoparticles were well-tolerated after oral administration in rats, as evidenced by by measuring levels of alanine aminotransferase, aspartate aminotransferases, albumin, creatinine and urea.
33019826	1	47	dep	levels	272:277	arg1	lower					252:256	lower	252:256	lower	252:256	The aims of the current study were to develop insulin-loaded nanoparticles comprised of various polymers at different compositions, and to evaluate their ability to lower blood glucose levels in diabetic rats following subcutaneous and oral administrations.
33019826	2	48	theme	Several	345:351	arg1	combinations					353:364	Several combinations	345:364	Several combinations of natural and synthetic polymers	345:398	Several combinations of natural and synthetic polymers have been utilized for preparation of nanoparticles including, chitosan, alginate, albumin and Pluronic.
33019826	6	49	theme	free	1172:1175	arg1	insulin					1177:1183	free insulin	1172:1183	free insulin	1172:1183	For instance, subcutaneous injection of nanoparticles comprised of chitosan combined with sodium tripolyphosphate, Pluronic or alginate/calcium chloride, resulted in comparable hypoglycemic effects to free insulin, at two-fold lower dose.
33019826	6	50	theme	tripolyphosphate	1068:1083	arg1	sodium					1061:1066	sodium	1061:1066	sodium tripolyphosphate, Pluronic	1061:1093	For instance, subcutaneous injection of nanoparticles comprised of chitosan combined with sodium tripolyphosphate, Pluronic or alginate/calcium chloride, resulted in comparable hypoglycemic effects to free insulin, at two-fold lower dose.
33019826	8	51	theme	ratio	1587:1591	arg1	fine-tuning					1560:1570	fine-tuning	1560:1570	fine-tuning of combination ratio between polymers	1560:1608	This study indicates that characteristics and delivery efficiency of nanomaterials can be controlled via utilizing several natural/synthetic polymers and by fine-tuning of combination ratio between polymers.
33019826	3	52	theme	spherical	531:539	arg1	170 nm-800 nm					516:528	170 nm-800 nm	516:528	170 nm-800 nm	516:528	Nanosized (170 nm-800 nm) spherical particles of high encapsulation efficiency (15-52%) have been prepared.
33019826	3	52	theme	spherical	531:539	arg1	particles					541:549	Nanosized (170 nm-800 nm) spherical particles	505:549	Nanosized (170 nm-800 nm) spherical particles of high encapsulation efficiency (15-52%)	505:591	Nanosized (170 nm-800 nm) spherical particles of high encapsulation efficiency (15-52%) have been prepared.
33019826	3	53	theme	Nanosized	505:513	arg1	170 nm-800 nm					516:528	170 nm-800 nm	516:528	170 nm-800 nm	516:528	Nanosized (170 nm-800 nm) spherical particles of high encapsulation efficiency (15-52%) have been prepared.
33019826	3	53	theme	Nanosized	505:513	arg1	particles					541:549	Nanosized (170 nm-800 nm) spherical particles	505:549	Nanosized (170 nm-800 nm) spherical particles of high encapsulation efficiency (15-52%)	505:591	Nanosized (170 nm-800 nm) spherical particles of high encapsulation efficiency (15-52%) have been prepared.
33019826	4	54	theme	hypoglycemic	739:750	arg1	activity					752:759	in vivo hypoglycemic activity	731:759	in vivo hypoglycemic activity	731:759	Composition and ratios between the integrated polymers played a pivotal role in determining size, zeta potential, and in vivo hypoglycemic activity of particles.
33019826	8	55	theme	combination	1575:1585	arg1	ratio					1587:1591	combination ratio	1575:1591	combination ratio between polymers	1575:1608	This study indicates that characteristics and delivery efficiency of nanomaterials can be controlled via utilizing several natural/synthetic polymers and by fine-tuning of combination ratio between polymers.
33019826	8	56	theme	several	1518:1524	arg1	polymers					1544:1551	several natural/synthetic polymers	1518:1551	several natural/synthetic polymers	1518:1551	This study indicates that characteristics and delivery efficiency of nanomaterials can be controlled via utilizing several natural/synthetic polymers and by fine-tuning of combination ratio between polymers.
33019826	6	57	theme	alginate/calcium	1098:1113	arg1	chloride					1115:1122	alginate/calcium chloride	1098:1122	alginate/calcium chloride	1098:1122	For instance, subcutaneous injection of nanoparticles comprised of chitosan combined with sodium tripolyphosphate, Pluronic or alginate/calcium chloride, resulted in comparable hypoglycemic effects to free insulin, at two-fold lower dose.
33019826	1	58	theme	insulin-loaded	133:146	arg1	nanoparticles					148:160	insulin-loaded nanoparticles	133:160	insulin-loaded nanoparticles comprised of various polymers at different compositions	133:216	The aims of the current study were to develop insulin-loaded nanoparticles comprised of various polymers at different compositions, and to evaluate their ability to lower blood glucose levels in diabetic rats following subcutaneous and oral administrations.
33019826	4	59	theme	pivotal	677:683	arg1	role					685:688	a pivotal role	675:688	a pivotal role	675:688	Composition and ratios between the integrated polymers played a pivotal role in determining size, zeta potential, and in vivo hypoglycemic activity of particles.
33019826	0	60	theme	insulin	69:75	arg1	delivery					77:84	in vivo insulin delivery	61:84	in vivo insulin delivery	61:84	Nanoparticles integrating natural and synthetic polymers for in vivo insulin delivery.
33019826	7	61	theme	oral	1250:1253	arg1	administration					1255:1268	oral administration	1250:1268	oral administration in rats	1250:1276	Nanoparticles were well-tolerated after oral administration in rats, as evidenced by by measuring levels of alanine aminotransferase, aspartate aminotransferases, albumin, creatinine and urea.
33019826	2	62	used	utilized	410:417	arg2	combinations					353:364	Several combinations	345:364	Several combinations of natural and synthetic polymers	345:398	Several combinations of natural and synthetic polymers have been utilized for preparation of nanoparticles including, chitosan, alginate, albumin and Pluronic.
33019826	2	63	theme	synthetic	381:389	arg1	polymers					391:398	natural and synthetic polymers	369:398	natural and synthetic polymers	369:398	Several combinations of natural and synthetic polymers have been utilized for preparation of nanoparticles including, chitosan, alginate, albumin and Pluronic.
32599239	5	0	theme	mRNA	818:821	arg1	levels					823:828	mRNA levels	818:828	mRNA levels	818:828	Protein expression and mRNA levels in NF-kB and Nrf2/HO-1 signaling pathways were detected by western blots and real-time polymerase chain reaction (RT-PCR), respectively.
32599239	3	1	with	β-D-Glcp	530:537	arg1	branches					572:579	the branches	568:579	the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp	568:682	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	6	2	theme	Nrf2-mediated	1096:1108	arg1	stress					1120:1125	Nrf2-mediated oxidative stress	1096:1125	Nrf2-mediated oxidative stress	1096:1125	The results strongly proved that SLPC-1S can be treated as a potential agent for preventing and treating diabetes via regulating Nrf2-mediated oxidative stress and NF-κB-mediated inflammatory responses.
32599239	4	3	theme	antidiabetic	726:737	arg1	activities					739:748	excellent antidiabetic activities	716:748	excellent antidiabetic activities in the streptozotocin-induced diabetic mice	716:792	Furthermore, SLPC-1S exhibited excellent antidiabetic activities in the streptozotocin-induced diabetic mice.
32599239	0	4	theme	antidiabetic	80:91	arg1	activity					93:100	its antidiabetic activity	76:100	its antidiabetic activity via Nrf2/HO-1 and NF-κB pathways	76:133	Structural characterization of a polysaccharide from Suillellus luridus and its antidiabetic activity via Nrf2/HO-1 and NF-κB pathways.
32599239	0	5	from	characterization	11:26	arg1	luridus					64:70	Suillellus luridus	53:70	Suillellus luridus	53:70	Structural characterization of a polysaccharide from Suillellus luridus and its antidiabetic activity via Nrf2/HO-1 and NF-κB pathways.
32599239	1	6	with	SLPC-1S	170:176	arg1	Mw					187:188	the Mw	183:188	the Mw of 9.4 kDa	183:199	A heteropolysaccharide designated SLPC-1S with the Mw of 9.4 kDa was purified from the caps of Suillellus luridus.
32599239	0	7	theme	Nrf2/HO-1	106:114	arg1	pathways					126:133	Nrf2/HO-1 and NF-κB pathways	106:133	Nrf2/HO-1 and NF-κB pathways	106:133	Structural characterization of a polysaccharide from Suillellus luridus and its antidiabetic activity via Nrf2/HO-1 and NF-κB pathways.
32599239	3	8	link	T-linked	666:673	arg1	α-D-Galp					675:682	T-linked α-D-Galp	666:682	T-linked α-D-Galp	666:682	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	6	9	theme	potential	1028:1036	arg1	agent					1038:1042	a potential agent	1026:1042	a potential agent for preventing and treating diabetes via regulating Nrf2-mediated oxidative stress and NF-κB-mediated inflammatory responses	1026:1167	The results strongly proved that SLPC-1S can be treated as a potential agent for preventing and treating diabetes via regulating Nrf2-mediated oxidative stress and NF-κB-mediated inflammatory responses.
32599239	6	9	theme	potential	1028:1036	arg1	SLPC-1S					1000:1006	SLPC-1S	1000:1006	SLPC-1S	1000:1006	The results strongly proved that SLPC-1S can be treated as a potential agent for preventing and treating diabetes via regulating Nrf2-mediated oxidative stress and NF-κB-mediated inflammatory responses.
32599239	0	10	from	luridus	64:70	arg1	activity					93:100	its antidiabetic activity	76:100	its antidiabetic activity via Nrf2/HO-1 and NF-κB pathways	76:133	Structural characterization of a polysaccharide from Suillellus luridus and its antidiabetic activity via Nrf2/HO-1 and NF-κB pathways.
32599239	0	10	from	luridus	64:70	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of a polysaccharide from Suillellus luridus	0:70	Structural characterization of a polysaccharide from Suillellus luridus and its antidiabetic activity via Nrf2/HO-1 and NF-κB pathways.
32599239	0	10	from	luridus	64:70	arg1	polysaccharide					33:46	a polysaccharide	31:46	a polysaccharide from Suillellus luridus	31:70	Structural characterization of a polysaccharide from Suillellus luridus and its antidiabetic activity via Nrf2/HO-1 and NF-κB pathways.
32599239	3	11	link	linked	502:507	arg1	α-D-Galp					509:516	1,3 linked α-D-Galp	498:516	1,3 linked α-D-Galp	498:516	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	5	12	theme	real-time	907:915	arg1	RT-PCR					944:949	RT-PCR	944:949	RT-PCR	944:949	Protein expression and mRNA levels in NF-kB and Nrf2/HO-1 signaling pathways were detected by western blots and real-time polymerase chain reaction (RT-PCR), respectively.
32599239	5	12	theme	real-time	907:915	arg1	reaction					934:941	real-time polymerase chain reaction	907:941	real-time polymerase chain reaction (RT-PCR)	907:950	Protein expression and mRNA levels in NF-kB and Nrf2/HO-1 signaling pathways were detected by western blots and real-time polymerase chain reaction (RT-PCR), respectively.
32599239	0	13	theme	NF-κB	120:124	arg1	pathways					126:133	Nrf2/HO-1 and NF-κB pathways	106:133	Nrf2/HO-1 and NF-κB pathways	106:133	Structural characterization of a polysaccharide from Suillellus luridus and its antidiabetic activity via Nrf2/HO-1 and NF-κB pathways.
32599239	3	14	with	α-D-Galp	509:516	arg1	branches					572:579	the branches	568:579	the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp	568:682	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	5	15	theme	western	889:895	arg1	blots					897:901	western blots	889:901	western blots	889:901	Protein expression and mRNA levels in NF-kB and Nrf2/HO-1 signaling pathways were detected by western blots and real-time polymerase chain reaction (RT-PCR), respectively.
32599239	1	16	theme	9.4 kDa	193:199	arg1	Mw					187:188	the Mw	183:188	the Mw of 9.4 kDa	183:199	A heteropolysaccharide designated SLPC-1S with the Mw of 9.4 kDa was purified from the caps of Suillellus luridus.
32599239	3	17	dep	linked	604:609	arg1	1,3					600:602	1,3	600:602	1,3	600:602	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	5	18	theme	Protein	795:801	arg1	expression					803:812	Protein expression	795:812	Protein expression	795:812	Protein expression and mRNA levels in NF-kB and Nrf2/HO-1 signaling pathways were detected by western blots and real-time polymerase chain reaction (RT-PCR), respectively.
32599239	5	19	theme	signaling	853:861	arg1	pathways					863:870	Nrf2/HO-1 signaling pathways	843:870	Nrf2/HO-1 signaling pathways	843:870	Protein expression and mRNA levels in NF-kB and Nrf2/HO-1 signaling pathways were detected by western blots and real-time polymerase chain reaction (RT-PCR), respectively.
32599239	3	20	with	β-D-Glcp	554:561	arg1	branches					572:579	the branches	568:579	the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp	568:682	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	3	21	theme	Structural	408:417	arg1	characterization					419:434	Structural characterization	408:434	Structural characterization	408:434	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	5	22	theme	polymerase	917:926	arg1	RT-PCR					944:949	RT-PCR	944:949	RT-PCR	944:949	Protein expression and mRNA levels in NF-kB and Nrf2/HO-1 signaling pathways were detected by western blots and real-time polymerase chain reaction (RT-PCR), respectively.
32599239	5	22	theme	polymerase	917:926	arg1	reaction					934:941	real-time polymerase chain reaction	907:941	real-time polymerase chain reaction (RT-PCR)	907:950	Protein expression and mRNA levels in NF-kB and Nrf2/HO-1 signaling pathways were detected by western blots and real-time polymerase chain reaction (RT-PCR), respectively.
32599239	3	23	link	linked	625:630	arg1	α-L-Arap					632:639	1,3 linked α-L-Arap	621:639	1,3 linked α-L-Arap	621:639	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	3	24	theme	linked	547:552	arg1	β-D-Glcp					554:561	1,6 linked β-D-Glcp	543:561	1,6 linked β-D-Glcp	543:561	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	6	25	theme	inflammatory	1146:1157	arg1	responses					1159:1167	NF-κB-mediated inflammatory responses	1131:1167	NF-κB-mediated inflammatory responses	1131:1167	The results strongly proved that SLPC-1S can be treated as a potential agent for preventing and treating diabetes via regulating Nrf2-mediated oxidative stress and NF-κB-mediated inflammatory responses.
32599239	3	26	dep	linked	523:528	arg1	1,3					519:521	1,3	519:521	1,3	519:521	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	2	27	theme	44.9:27.6:14.7:12.8	387:405	arg1	ratio					378:382	a molar ratio	370:382	a molar ratio of 44.9:27.6:14.7:12.8	370:405	Monosaccharide composition analysis revealed that SLPC-1S was composed of galactose, glucose, arabinose and mannose in a molar ratio of 44.9:27.6:14.7:12.8.
32599239	0	28	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of a polysaccharide from Suillellus luridus	0:70	Structural characterization of a polysaccharide from Suillellus luridus and its antidiabetic activity via Nrf2/HO-1 and NF-κB pathways.
32599239	4	29	theme	diabetic	780:787	arg1	mice					789:792	the streptozotocin-induced diabetic mice	753:792	the streptozotocin-induced diabetic mice	753:792	Furthermore, SLPC-1S exhibited excellent antidiabetic activities in the streptozotocin-induced diabetic mice.
32599239	4	30	theme	excellent	716:724	arg1	activities					739:748	excellent antidiabetic activities	716:748	excellent antidiabetic activities in the streptozotocin-induced diabetic mice	716:792	Furthermore, SLPC-1S exhibited excellent antidiabetic activities in the streptozotocin-induced diabetic mice.
32599239	3	31	theme	linked	625:630	arg1	α-L-Arap					632:639	1,3 linked α-L-Arap	621:639	1,3 linked α-L-Arap	621:639	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	6	32	theme	NF-κB-mediated	1131:1144	arg1	responses					1159:1167	NF-κB-mediated inflammatory responses	1131:1167	NF-κB-mediated inflammatory responses	1131:1167	The results strongly proved that SLPC-1S can be treated as a potential agent for preventing and treating diabetes via regulating Nrf2-mediated oxidative stress and NF-κB-mediated inflammatory responses.
32599239	3	33	dep	linked	625:630	arg1	1,3					621:623	1,3	621:623	1,3	621:623	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	0	34	from	activity	93:100	arg1	luridus					64:70	Suillellus luridus	53:70	Suillellus luridus	53:70	Structural characterization of a polysaccharide from Suillellus luridus and its antidiabetic activity via Nrf2/HO-1 and NF-κB pathways.
32599239	3	35	dep	linked	547:552	arg1	1,6					543:545	1,6	543:545	1,6	543:545	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	3	36	theme	linked	502:507	arg1	α-D-Galp					509:516	1,3 linked α-D-Galp	498:516	1,3 linked α-D-Galp	498:516	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	3	37	link	linked	604:609	arg1	β-D-Glcp					611:618	1,3 linked β-D-Glcp	600:618	1,3 linked β-D-Glcp	600:618	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	3	38	theme	T-linked	666:673	arg1	α-D-Galp					675:682	T-linked α-D-Galp	666:682	T-linked α-D-Galp	666:682	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	0	39	theme	polysaccharide	33:46	arg1	activity					93:100	its antidiabetic activity	76:100	its antidiabetic activity via Nrf2/HO-1 and NF-κB pathways	76:133	Structural characterization of a polysaccharide from Suillellus luridus and its antidiabetic activity via Nrf2/HO-1 and NF-κB pathways.
32599239	0	39	theme	polysaccharide	33:46	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of a polysaccharide from Suillellus luridus	0:70	Structural characterization of a polysaccharide from Suillellus luridus and its antidiabetic activity via Nrf2/HO-1 and NF-κB pathways.
32599239	3	40	link	linked	547:552	arg1	β-D-Glcp					554:561	1,6 linked β-D-Glcp	543:561	1,6 linked β-D-Glcp	543:561	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	3	41	theme	linked	646:651	arg1	α-D-Manp					653:660	1,3 linked α-D-Manp	642:660	1,3 linked α-D-Manp	642:660	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	5	42	theme	chain	928:932	arg1	RT-PCR					944:949	RT-PCR	944:949	RT-PCR	944:949	Protein expression and mRNA levels in NF-kB and Nrf2/HO-1 signaling pathways were detected by western blots and real-time polymerase chain reaction (RT-PCR), respectively.
32599239	5	42	theme	chain	928:932	arg1	reaction					934:941	real-time polymerase chain reaction	907:941	real-time polymerase chain reaction (RT-PCR)	907:950	Protein expression and mRNA levels in NF-kB and Nrf2/HO-1 signaling pathways were detected by western blots and real-time polymerase chain reaction (RT-PCR), respectively.
32599239	3	43	theme	linked	604:609	arg1	β-D-Glcp					611:618	1,3 linked β-D-Glcp	600:618	1,3 linked β-D-Glcp	600:618	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	5	44	theme	Nrf2/HO-1	843:851	arg1	pathways					863:870	Nrf2/HO-1 signaling pathways	843:870	Nrf2/HO-1 signaling pathways	843:870	Protein expression and mRNA levels in NF-kB and Nrf2/HO-1 signaling pathways were detected by western blots and real-time polymerase chain reaction (RT-PCR), respectively.
32599239	5	45	from	levels	823:828	arg1	pathways					863:870	Nrf2/HO-1 signaling pathways	843:870	Nrf2/HO-1 signaling pathways	843:870	Protein expression and mRNA levels in NF-kB and Nrf2/HO-1 signaling pathways were detected by western blots and real-time polymerase chain reaction (RT-PCR), respectively.
32599239	5	45	from	levels	823:828	arg1	NF-kB					833:837	NF-kB	833:837	NF-kB	833:837	Protein expression and mRNA levels in NF-kB and Nrf2/HO-1 signaling pathways were detected by western blots and real-time polymerase chain reaction (RT-PCR), respectively.
32599239	3	46	theme	linked	523:528	arg1	β-D-Glcp					530:537	1,3 linked β-D-Glcp	519:537	1,3 linked β-D-Glcp	519:537	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	3	47	dep	linked	502:507	arg1	1,3					498:500	1,3	498:500	1,3	498:500	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	3	48	contain	had	459:461	arg2	backbone					465:472	a backbone	463:472	a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp	463:682	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	3	48	contain	had	459:461	arg1	SLPC-1S					451:457	SLPC-1S	451:457	SLPC-1S	451:457	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	4	49	theme	streptozotocin-induced	757:778	arg1	mice					789:792	the streptozotocin-induced diabetic mice	753:792	the streptozotocin-induced diabetic mice	753:792	Furthermore, SLPC-1S exhibited excellent antidiabetic activities in the streptozotocin-induced diabetic mice.
32599239	3	50	dep	linked	646:651	arg1	1,3					642:644	1,3	642:644	1,3	642:644	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	3	51	link	linked	523:528	arg1	β-D-Glcp					530:537	1,3 linked β-D-Glcp	519:537	1,3 linked β-D-Glcp	519:537	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32599239	5	52	from	expression	803:812	arg1	pathways					863:870	Nrf2/HO-1 signaling pathways	843:870	Nrf2/HO-1 signaling pathways	843:870	Protein expression and mRNA levels in NF-kB and Nrf2/HO-1 signaling pathways were detected by western blots and real-time polymerase chain reaction (RT-PCR), respectively.
32599239	5	52	from	expression	803:812	arg1	NF-kB					833:837	NF-kB	833:837	NF-kB	833:837	Protein expression and mRNA levels in NF-kB and Nrf2/HO-1 signaling pathways were detected by western blots and real-time polymerase chain reaction (RT-PCR), respectively.
32599239	1	53	theme	heteropolysaccharide	138:157	arg1	SLPC-1S					170:176	A heteropolysaccharide designated SLPC-1S	136:176	A heteropolysaccharide designated SLPC-1S with the Mw of 9.4 kDa	136:199	A heteropolysaccharide designated SLPC-1S with the Mw of 9.4 kDa was purified from the caps of Suillellus luridus.
32599239	2	54	theme	composition	266:276	arg1	analysis					278:285	Monosaccharide composition analysis	251:285	Monosaccharide composition analysis	251:285	Monosaccharide composition analysis revealed that SLPC-1S was composed of galactose, glucose, arabinose and mannose in a molar ratio of 44.9:27.6:14.7:12.8.
32599239	6	55	theme	oxidative	1110:1118	arg1	stress					1120:1125	Nrf2-mediated oxidative stress	1096:1125	Nrf2-mediated oxidative stress	1096:1125	The results strongly proved that SLPC-1S can be treated as a potential agent for preventing and treating diabetes via regulating Nrf2-mediated oxidative stress and NF-κB-mediated inflammatory responses.
32599239	1	56	theme	designated	159:168	arg1	SLPC-1S					170:176	A heteropolysaccharide designated SLPC-1S	136:176	A heteropolysaccharide designated SLPC-1S with the Mw of 9.4 kDa	136:199	A heteropolysaccharide designated SLPC-1S with the Mw of 9.4 kDa was purified from the caps of Suillellus luridus.
32599239	4	57	from	activities	739:748	arg1	mice					789:792	the streptozotocin-induced diabetic mice	753:792	the streptozotocin-induced diabetic mice	753:792	Furthermore, SLPC-1S exhibited excellent antidiabetic activities in the streptozotocin-induced diabetic mice.
32599239	1	58	theme	luridus	242:248	arg1	caps					223:226	the caps	219:226	the caps of Suillellus luridus	219:248	A heteropolysaccharide designated SLPC-1S with the Mw of 9.4 kDa was purified from the caps of Suillellus luridus.
32599239	2	59	theme	Monosaccharide	251:264	arg1	analysis					278:285	Monosaccharide composition analysis	251:285	Monosaccharide composition analysis	251:285	Monosaccharide composition analysis revealed that SLPC-1S was composed of galactose, glucose, arabinose and mannose in a molar ratio of 44.9:27.6:14.7:12.8.
32599239	2	60	theme	molar	372:376	arg1	ratio					378:382	a molar ratio	370:382	a molar ratio of 44.9:27.6:14.7:12.8	370:405	Monosaccharide composition analysis revealed that SLPC-1S was composed of galactose, glucose, arabinose and mannose in a molar ratio of 44.9:27.6:14.7:12.8.
32599239	3	61	link	linked	646:651	arg1	α-D-Manp					653:660	1,3 linked α-D-Manp	642:660	1,3 linked α-D-Manp	642:660	Structural characterization indicated that SLPC-1S had a backbone principally composed of 1,3 linked α-D-Galp, 1,3 linked β-D-Glcp and 1,6 linked β-D-Glcp with the branches mainly composed of 1,3 linked β-D-Glcp, 1,3 linked α-L-Arap, 1,3 linked α-D-Manp and T-linked α-D-Galp.
32702068	0	0	theme	baking	79:84	arg1	industry					86:93	the baking industry	75:93	the baking industry	75:93	Selection of cereal-sourced lactic acid bacteria as candidate starters for the baking industry.
32702068	7	1	theme	EPS	1148:1150	arg1	producers					1152:1160	Almost all the EPS producers	1133:1160	Almost all the EPS producers	1133:1160	Almost all the EPS producers carried one or more genes (epsD/E and/or epsA) involved in the production of heteropolysaccharides (HePS), whereas the isolates ascribed to Leuconostoc citreum and W. confusa carried genes involved in the production of both HePS and homopolysaccharides (HoPS).
32702068	4	2	theme	screened	661:668	arg1	isolates					670:677	the screened isolates	657:677	the screened isolates	657:677	There was large heterogeneity in the phytase activity observed among the screened isolates, with 95% showing the ability to degrade sodium phytate on plates containing Sourdough Simulation Medium (SSM).
32702068	7	3	contain	carried	1162:1168	arg1	producers					1152:1160	Almost all the EPS producers	1133:1160	Almost all the EPS producers	1133:1160	Almost all the EPS producers carried one or more genes (epsD/E and/or epsA) involved in the production of heteropolysaccharides (HePS), whereas the isolates ascribed to Leuconostoc citreum and W. confusa carried genes involved in the production of both HePS and homopolysaccharides (HoPS).
32702068	7	3	contain	carried	1162:1168	arg2	genes					1182:1186	one or more genes	1170:1186	one or more genes (epsD/E and/or epsA) involved in the production of heteropolysaccharides (HePS)	1170:1266	Almost all the EPS producers carried one or more genes (epsD/E and/or epsA) involved in the production of heteropolysaccharides (HePS), whereas the isolates ascribed to Leuconostoc citreum and W. confusa carried genes involved in the production of both HePS and homopolysaccharides (HoPS).
32702068	1	4	theme	metabolic	145:153	arg1	activities					155:164	metabolic activities	145:164	metabolic activities of lactic acid bacteria (LAB)	145:194	The quality of sourdough bread mainly depends on metabolic activities of lactic acid bacteria (LAB).
32702068	9	5	theme	flour	1770:1774	arg1	medium					1776:1781	an ad hoc formulated barley flour medium	1742:1781	an ad hoc formulated barley flour medium	1742:1781	Furthermore, a few isolates ascribed to L. citreum and W. confusa and carrying the gtf gene produced β-glucans after fermentation in an ad hoc formulated barley flour medium.
32702068	5	6	theme	highest	875:881	arg1	activity					893:900	the highest enzymatic activity	871:900	the highest enzymatic activity	871:900	The isolates Lactobacillus brevis LD65 and Lactobacillus plantarum PB241 showed the highest enzymatic activity, while the isolates ascribed to Weissella confusa were characterized by low or no phytase activity.
32702068	7	7	theme	HePS	1386:1389	arg1	production					1367:1376	the production	1363:1376	the production of both HePS and homopolysaccharides (HoPS)	1363:1420	Almost all the EPS producers carried one or more genes (epsD/E and/or epsA) involved in the production of heteropolysaccharides (HePS), whereas the isolates ascribed to Leuconostoc citreum and W. confusa carried genes involved in the production of both HePS and homopolysaccharides (HoPS).
32702068	4	8	from	phytate	727:733	arg1	plates					738:743	plates	738:743	plates containing Sourdough Simulation Medium (SSM)	738:788	There was large heterogeneity in the phytase activity observed among the screened isolates, with 95% showing the ability to degrade sodium phytate on plates containing Sourdough Simulation Medium (SSM).
32702068	8	9	theme	different	1591:1599	arg1	ratios					1601:1606	different ratios	1591:1606	different ratios	1591:1606	Monosaccharide composition analysis of the EPS produced by a selected subset of isolates revealed that all the HePS included glucose, mannose, and galactose, though at different ratios.
32702068	11	10	theme	phytase	1977:1983	arg1	activity					1985:1992	high phytase activity	1972:1992	high phytase activity	1972:1992	brevis LD66 and L. citreum PB220, which showed high phytase activity and positive EPS production.
32702068	9	11	dep	isolates	1628:1635	arg1	carrying					1679:1686	carrying	1679:1686	carrying the gtf gene	1679:1699	Furthermore, a few isolates ascribed to L. citreum and W. confusa and carrying the gtf gene produced β-glucans after fermentation in an ad hoc formulated barley flour medium.
32702068	9	11	dep	isolates	1628:1635	arg1	ascribed					1637:1644	ascribed	1637:1644	ascribed to L. citreum and W. confusa	1637:1673	Furthermore, a few isolates ascribed to L. citreum and W. confusa and carrying the gtf gene produced β-glucans after fermentation in an ad hoc formulated barley flour medium.
32702068	5	12	theme	phytase	984:990	arg1	activity					992:999	phytase activity	984:999	phytase activity	984:999	The isolates Lactobacillus brevis LD65 and Lactobacillus plantarum PB241 showed the highest enzymatic activity, while the isolates ascribed to Weissella confusa were characterized by low or no phytase activity.
32702068	2	13	theme	technological	264:276	arg1	properties					294:303	the technological and nutritional properties	260:303	the technological and nutritional properties of the bread	260:316	The exopolysaccharides (EPS) produced by LAB affect positively the technological and nutritional properties of the bread, while phytases improve the bioavailability of the minerals by reducing its phytate content.
32702068	7	14	dep	Leuconostoc	1302:1312	arg1	citreum					1314:1320	Leuconostoc citreum	1302:1320	Leuconostoc citreum	1302:1320	Almost all the EPS producers carried one or more genes (epsD/E and/or epsA) involved in the production of heteropolysaccharides (HePS), whereas the isolates ascribed to Leuconostoc citreum and W. confusa carried genes involved in the production of both HePS and homopolysaccharides (HoPS).
32702068	11	15	theme	high	1972:1975	arg1	activity					1985:1992	high phytase activity	1972:1992	high phytase activity	1972:1992	brevis LD66 and L. citreum PB220, which showed high phytase activity and positive EPS production.
32702068	3	16	theme	EPS	511:513	arg1	production					484:493	production	484:493	production of phytases and EPS	484:513	In the present study, a pool of 152 cereal-sourced LAB were screened for production of phytases and EPS for potential use as sourdough starter cultures for the baking industry.
32702068	10	17	theme	starter	1856:1862	arg1	cultures					1864:1871	candidate sourdough starter cultures	1836:1871	candidate sourdough starter cultures for the baking industry	1836:1895	Based on the overall results collected, a subset of candidate sourdough starter cultures for the baking industry was selected, including Lb.
32702068	10	18	theme	baking	1881:1886	arg1	industry					1888:1895	the baking industry	1877:1895	the baking industry	1877:1895	Based on the overall results collected, a subset of candidate sourdough starter cultures for the baking industry was selected, including Lb.
32702068	6	19	theme	ropy	1073:1076	arg1	phenotypes					1088:1097	ropy or mucoid phenotypes	1073:1097	ropy or mucoid phenotypes	1073:1097	Only 18% of the screened LAB produced EPS, which were distinguished as ropy or mucoid phenotypes on SSM supplemented with sucrose.
32702068	6	19	theme	ropy	1073:1076	arg1	EPS					1040:1042	EPS	1040:1042	EPS	1040:1042	Only 18% of the screened LAB produced EPS, which were distinguished as ropy or mucoid phenotypes on SSM supplemented with sucrose.
32702068	11	20	theme	EPS	2007:2009	arg1	production					2011:2020	positive EPS production	1998:2020	positive EPS production	1998:2020	brevis LD66 and L. citreum PB220, which showed high phytase activity and positive EPS production.
32702068	5	21	dep	low	974:976	arg1	activity					992:999	phytase activity	984:999	phytase activity	984:999	The isolates Lactobacillus brevis LD65 and Lactobacillus plantarum PB241 showed the highest enzymatic activity, while the isolates ascribed to Weissella confusa were characterized by low or no phytase activity.
32702068	5	22	theme	Weissella	934:942	arg1	confusa					944:950	Weissella confusa	934:950	Weissella confusa	934:950	The isolates Lactobacillus brevis LD65 and Lactobacillus plantarum PB241 showed the highest enzymatic activity, while the isolates ascribed to Weissella confusa were characterized by low or no phytase activity.
32702068	5	23	theme	enzymatic	883:891	arg1	activity					893:900	the highest enzymatic activity	871:900	the highest enzymatic activity	871:900	The isolates Lactobacillus brevis LD65 and Lactobacillus plantarum PB241 showed the highest enzymatic activity, while the isolates ascribed to Weissella confusa were characterized by low or no phytase activity.
32702068	6	24	theme	LAB	1027:1029	arg1	%					1009:1009	Only 18%	1002:1009	Only 18% of the screened LAB	1002:1029	Only 18% of the screened LAB produced EPS, which were distinguished as ropy or mucoid phenotypes on SSM supplemented with sucrose.
32702068	6	24	theme	LAB	1027:1029	arg1	LAB					1027:1029	the screened LAB	1014:1029	the screened LAB	1014:1029	Only 18% of the screened LAB produced EPS, which were distinguished as ropy or mucoid phenotypes on SSM supplemented with sucrose.
32702068	3	25	theme	phytases	498:505	arg1	production					484:493	production	484:493	production of phytases and EPS	484:513	In the present study, a pool of 152 cereal-sourced LAB were screened for production of phytases and EPS for potential use as sourdough starter cultures for the baking industry.
32702068	1	26	theme	lactic	169:174	arg1	LAB					191:193	LAB	191:193	LAB	191:193	The quality of sourdough bread mainly depends on metabolic activities of lactic acid bacteria (LAB).
32702068	1	26	theme	lactic	169:174	arg1	bacteria					181:188	lactic acid bacteria	169:188	lactic acid bacteria (LAB)	169:194	The quality of sourdough bread mainly depends on metabolic activities of lactic acid bacteria (LAB).
32702068	7	27	dep	genes	1182:1186	arg1	epsA					1203:1206	epsA	1203:1206	epsA	1203:1206	Almost all the EPS producers carried one or more genes (epsD/E and/or epsA) involved in the production of heteropolysaccharides (HePS), whereas the isolates ascribed to Leuconostoc citreum and W. confusa carried genes involved in the production of both HePS and homopolysaccharides (HoPS).
32702068	7	27	dep	genes	1182:1186	arg1	epsD/E					1189:1194	epsD/E	1189:1194	epsD/E	1189:1194	Almost all the EPS producers carried one or more genes (epsD/E and/or epsA) involved in the production of heteropolysaccharides (HePS), whereas the isolates ascribed to Leuconostoc citreum and W. confusa carried genes involved in the production of both HePS and homopolysaccharides (HoPS).
32702068	9	28	theme	gtf	1692:1694	arg1	gene					1696:1699	the gtf gene	1688:1699	the gtf gene	1688:1699	Furthermore, a few isolates ascribed to L. citreum and W. confusa and carrying the gtf gene produced β-glucans after fermentation in an ad hoc formulated barley flour medium.
32702068	8	29	theme	composition	1438:1448	arg1	analysis					1450:1457	Monosaccharide composition analysis	1423:1457	Monosaccharide composition analysis of the EPS produced by a selected subset of isolates	1423:1510	Monosaccharide composition analysis of the EPS produced by a selected subset of isolates revealed that all the HePS included glucose, mannose, and galactose, though at different ratios.
32702068	1	30	theme	acid	176:179	arg1	LAB					191:193	LAB	191:193	LAB	191:193	The quality of sourdough bread mainly depends on metabolic activities of lactic acid bacteria (LAB).
32702068	1	30	theme	acid	176:179	arg1	bacteria					181:188	lactic acid bacteria	169:188	lactic acid bacteria (LAB)	169:194	The quality of sourdough bread mainly depends on metabolic activities of lactic acid bacteria (LAB).
32702068	8	31	theme	Monosaccharide	1423:1436	arg1	analysis					1450:1457	Monosaccharide composition analysis	1423:1457	Monosaccharide composition analysis of the EPS produced by a selected subset of isolates	1423:1510	Monosaccharide composition analysis of the EPS produced by a selected subset of isolates revealed that all the HePS included glucose, mannose, and galactose, though at different ratios.
32702068	7	32	dep	W.	1326:1327	arg1	confusa					1329:1335	W. confusa	1326:1335	W. confusa	1326:1335	Almost all the EPS producers carried one or more genes (epsD/E and/or epsA) involved in the production of heteropolysaccharides (HePS), whereas the isolates ascribed to Leuconostoc citreum and W. confusa carried genes involved in the production of both HePS and homopolysaccharides (HoPS).
32702068	11	33	theme	positive	1998:2005	arg1	production					2011:2020	positive EPS production	1998:2020	positive EPS production	1998:2020	brevis LD66 and L. citreum PB220, which showed high phytase activity and positive EPS production.
32702068	4	34	from	heterogeneity	604:616	arg1	activity					633:640	the phytase activity	621:640	the phytase activity observed among the screened isolates	621:677	There was large heterogeneity in the phytase activity observed among the screened isolates, with 95% showing the ability to degrade sodium phytate on plates containing Sourdough Simulation Medium (SSM).
32702068	1	35	theme	bacteria	181:188	arg1	activities					155:164	metabolic activities	145:164	metabolic activities of lactic acid bacteria (LAB)	145:194	The quality of sourdough bread mainly depends on metabolic activities of lactic acid bacteria (LAB).
32702068	0	36	theme	lactic	28:33	arg1	bacteria					40:47	cereal-sourced lactic acid bacteria	13:47	cereal-sourced lactic acid bacteria as candidate starters	13:69	Selection of cereal-sourced lactic acid bacteria as candidate starters for the baking industry.
32702068	6	37	theme	screened	1018:1025	arg1	LAB					1027:1029	the screened LAB	1014:1029	the screened LAB	1014:1029	Only 18% of the screened LAB produced EPS, which were distinguished as ropy or mucoid phenotypes on SSM supplemented with sucrose.
32702068	2	38	theme	nutritional	282:292	arg1	properties					294:303	the technological and nutritional properties	260:303	the technological and nutritional properties of the bread	260:316	The exopolysaccharides (EPS) produced by LAB affect positively the technological and nutritional properties of the bread, while phytases improve the bioavailability of the minerals by reducing its phytate content.
32702068	0	39	theme	cereal-sourced	13:26	arg1	bacteria					40:47	cereal-sourced lactic acid bacteria	13:47	cereal-sourced lactic acid bacteria as candidate starters	13:69	Selection of cereal-sourced lactic acid bacteria as candidate starters for the baking industry.
32702068	3	40	theme	potential	519:527	arg1	use					529:531	potential use	519:531	potential use as sourdough starter cultures for the baking industry	519:585	In the present study, a pool of 152 cereal-sourced LAB were screened for production of phytases and EPS for potential use as sourdough starter cultures for the baking industry.
32702068	2	41	theme	phytate	394:400	arg1	content					402:408	its phytate content	390:408	its phytate content	390:408	The exopolysaccharides (EPS) produced by LAB affect positively the technological and nutritional properties of the bread, while phytases improve the bioavailability of the minerals by reducing its phytate content.
32702068	0	42	theme	bacteria	40:47	arg1	Selection					0:8	Selection	0:8	Selection of cereal-sourced lactic acid bacteria as candidate starters for the baking industry	0:93	Selection of cereal-sourced lactic acid bacteria as candidate starters for the baking industry.
32702068	3	43	theme	LAB	462:464	arg1	pool					435:438	a pool	433:438	a pool of 152 cereal-sourced LAB	433:464	In the present study, a pool of 152 cereal-sourced LAB were screened for production of phytases and EPS for potential use as sourdough starter cultures for the baking industry.
32702068	7	44	dep	carried	1162:1168	arg1	whereas					1269:1275	whereas	1269:1275	whereas	1269:1275	Almost all the EPS producers carried one or more genes (epsD/E and/or epsA) involved in the production of heteropolysaccharides (HePS), whereas the isolates ascribed to Leuconostoc citreum and W. confusa carried genes involved in the production of both HePS and homopolysaccharides (HoPS).
32702068	4	45	theme	phytase	625:631	arg1	activity					633:640	the phytase activity	621:640	the phytase activity observed among the screened isolates	621:677	There was large heterogeneity in the phytase activity observed among the screened isolates, with 95% showing the ability to degrade sodium phytate on plates containing Sourdough Simulation Medium (SSM).
32702068	0	46	theme	acid	35:38	arg1	bacteria					40:47	cereal-sourced lactic acid bacteria	13:47	cereal-sourced lactic acid bacteria as candidate starters	13:69	Selection of cereal-sourced lactic acid bacteria as candidate starters for the baking industry.
32702068	4	47	theme	Simulation	766:775	arg1	Medium					777:782	Sourdough Simulation Medium	756:782	Sourdough Simulation Medium (SSM)	756:788	There was large heterogeneity in the phytase activity observed among the screened isolates, with 95% showing the ability to degrade sodium phytate on plates containing Sourdough Simulation Medium (SSM).
32702068	4	47	theme	Simulation	766:775	arg1	SSM					785:787	SSM	785:787	SSM	785:787	There was large heterogeneity in the phytase activity observed among the screened isolates, with 95% showing the ability to degrade sodium phytate on plates containing Sourdough Simulation Medium (SSM).
32702068	4	48	theme	large	598:602	arg1	heterogeneity					604:616	large heterogeneity	598:616	large heterogeneity in the phytase activity observed among the screened isolates	598:677	There was large heterogeneity in the phytase activity observed among the screened isolates, with 95% showing the ability to degrade sodium phytate on plates containing Sourdough Simulation Medium (SSM).
32702068	0	49	theme	candidate	52:60	arg1	starters					62:69	candidate starters	52:69	candidate starters	52:69	Selection of cereal-sourced lactic acid bacteria as candidate starters for the baking industry.
32702068	7	50	contain	carried	1337:1343	arg2	genes					1345:1349	genes	1345:1349	genes involved in the production of both HePS and homopolysaccharides (HoPS)	1345:1420	Almost all the EPS producers carried one or more genes (epsD/E and/or epsA) involved in the production of heteropolysaccharides (HePS), whereas the isolates ascribed to Leuconostoc citreum and W. confusa carried genes involved in the production of both HePS and homopolysaccharides (HoPS).
32702068	7	50	contain	carried	1337:1343	arg1	isolates					1281:1288	the isolates	1277:1288	the isolates ascribed to Leuconostoc citreum and W. confusa	1277:1335	Almost all the EPS producers carried one or more genes (epsD/E and/or epsA) involved in the production of heteropolysaccharides (HePS), whereas the isolates ascribed to Leuconostoc citreum and W. confusa carried genes involved in the production of both HePS and homopolysaccharides (HoPS).
32702068	10	51	theme	cultures	1864:1871	arg1	subset					1826:1831	a subset	1824:1831	a subset of candidate sourdough starter cultures for the baking industry	1824:1895	Based on the overall results collected, a subset of candidate sourdough starter cultures for the baking industry was selected, including Lb.
32702068	8	52	theme	EPS	1466:1468	arg1	analysis					1450:1457	Monosaccharide composition analysis	1423:1457	Monosaccharide composition analysis of the EPS produced by a selected subset of isolates	1423:1510	Monosaccharide composition analysis of the EPS produced by a selected subset of isolates revealed that all the HePS included glucose, mannose, and galactose, though at different ratios.
32702068	3	53	theme	sourdough	536:544	arg1	cultures					554:561	sourdough starter cultures	536:561	sourdough starter cultures	536:561	In the present study, a pool of 152 cereal-sourced LAB were screened for production of phytases and EPS for potential use as sourdough starter cultures for the baking industry.
32702068	3	54	theme	cereal-sourced	447:460	arg1	LAB					462:464	152 cereal-sourced LAB	443:464	152 cereal-sourced LAB	443:464	In the present study, a pool of 152 cereal-sourced LAB were screened for production of phytases and EPS for potential use as sourdough starter cultures for the baking industry.
32702068	8	55	theme	isolates	1503:1510	arg1	subset					1493:1498	a selected subset	1482:1498	a selected subset of isolates	1482:1510	Monosaccharide composition analysis of the EPS produced by a selected subset of isolates revealed that all the HePS included glucose, mannose, and galactose, though at different ratios.
32702068	7	56	theme	heteropolysaccharides	1239:1259	arg1	production					1225:1234	the production	1221:1234	the production of heteropolysaccharides (HePS)	1221:1266	Almost all the EPS producers carried one or more genes (epsD/E and/or epsA) involved in the production of heteropolysaccharides (HePS), whereas the isolates ascribed to Leuconostoc citreum and W. confusa carried genes involved in the production of both HePS and homopolysaccharides (HoPS).
32702068	3	57	theme	starter	546:552	arg1	cultures					554:561	sourdough starter cultures	536:561	sourdough starter cultures	536:561	In the present study, a pool of 152 cereal-sourced LAB were screened for production of phytases and EPS for potential use as sourdough starter cultures for the baking industry.
32702068	10	58	theme	overall	1797:1803	arg1	results					1805:1811	the overall results	1793:1811	the overall results collected	1793:1821	Based on the overall results collected, a subset of candidate sourdough starter cultures for the baking industry was selected, including Lb.
32702068	1	59	theme	sourdough	111:119	arg1	bread					121:125	sourdough bread	111:125	sourdough bread	111:125	The quality of sourdough bread mainly depends on metabolic activities of lactic acid bacteria (LAB).
32702068	10	60	theme	sourdough	1846:1854	arg1	cultures					1864:1871	candidate sourdough starter cultures	1836:1871	candidate sourdough starter cultures for the baking industry	1836:1895	Based on the overall results collected, a subset of candidate sourdough starter cultures for the baking industry was selected, including Lb.
32702068	9	61	theme	formulated	1752:1761	arg1	medium					1776:1781	an ad hoc formulated barley flour medium	1742:1781	an ad hoc formulated barley flour medium	1742:1781	Furthermore, a few isolates ascribed to L. citreum and W. confusa and carrying the gtf gene produced β-glucans after fermentation in an ad hoc formulated barley flour medium.
32702068	9	62	theme	few	1624:1626	arg1	isolates					1628:1635	a few isolates	1622:1635	a few isolates ascribed to L. citreum and W. confusa and carrying the gtf gene	1622:1699	Furthermore, a few isolates ascribed to L. citreum and W. confusa and carrying the gtf gene produced β-glucans after fermentation in an ad hoc formulated barley flour medium.
32702068	11	63	dep	citreum	1944:1950	arg1	showed					1965:1970	showed	1965:1970	showed high phytase activity and positive EPS production	1965:2020	brevis LD66 and L. citreum PB220, which showed high phytase activity and positive EPS production.
32702068	1	64	theme	bread	121:125	arg1	quality					100:106	The quality	96:106	The quality of sourdough bread	96:125	The quality of sourdough bread mainly depends on metabolic activities of lactic acid bacteria (LAB).
32702068	6	65	theme	mucoid	1081:1086	arg1	phenotypes					1088:1097	ropy or mucoid phenotypes	1073:1097	ropy or mucoid phenotypes	1073:1097	Only 18% of the screened LAB produced EPS, which were distinguished as ropy or mucoid phenotypes on SSM supplemented with sucrose.
32702068	6	65	theme	mucoid	1081:1086	arg1	EPS					1040:1042	EPS	1040:1042	EPS	1040:1042	Only 18% of the screened LAB produced EPS, which were distinguished as ropy or mucoid phenotypes on SSM supplemented with sucrose.
32702068	4	66	contain	containing	745:754	arg2	SSM					785:787	SSM	785:787	SSM	785:787	There was large heterogeneity in the phytase activity observed among the screened isolates, with 95% showing the ability to degrade sodium phytate on plates containing Sourdough Simulation Medium (SSM).
32702068	4	66	contain	containing	745:754	arg2	Medium					777:782	Sourdough Simulation Medium	756:782	Sourdough Simulation Medium (SSM)	756:788	There was large heterogeneity in the phytase activity observed among the screened isolates, with 95% showing the ability to degrade sodium phytate on plates containing Sourdough Simulation Medium (SSM).
32702068	4	66	contain	containing	745:754	arg1	plates					738:743	plates	738:743	plates containing Sourdough Simulation Medium (SSM)	738:788	There was large heterogeneity in the phytase activity observed among the screened isolates, with 95% showing the ability to degrade sodium phytate on plates containing Sourdough Simulation Medium (SSM).
32702068	4	67	theme	sodium	720:725	arg1	phytate					727:733	sodium phytate	720:733	sodium phytate on plates containing Sourdough Simulation Medium (SSM)	720:788	There was large heterogeneity in the phytase activity observed among the screened isolates, with 95% showing the ability to degrade sodium phytate on plates containing Sourdough Simulation Medium (SSM).
32702068	5	68	dep	isolates	795:802	arg1	brevis					818:823	Lactobacillus brevis	804:823	Lactobacillus brevis LD65	804:828	The isolates Lactobacillus brevis LD65 and Lactobacillus plantarum PB241 showed the highest enzymatic activity, while the isolates ascribed to Weissella confusa were characterized by low or no phytase activity.
32702068	5	68	dep	isolates	795:802	arg1	plantarum					848:856	Lactobacillus plantarum	834:856	Lactobacillus plantarum PB241	834:862	The isolates Lactobacillus brevis LD65 and Lactobacillus plantarum PB241 showed the highest enzymatic activity, while the isolates ascribed to Weissella confusa were characterized by low or no phytase activity.
32702068	9	69	theme	hoc	1748:1750	arg1	medium					1776:1781	an ad hoc formulated barley flour medium	1742:1781	an ad hoc formulated barley flour medium	1742:1781	Furthermore, a few isolates ascribed to L. citreum and W. confusa and carrying the gtf gene produced β-glucans after fermentation in an ad hoc formulated barley flour medium.
32702068	2	70	theme	bread	312:316	arg1	properties					294:303	the technological and nutritional properties	260:303	the technological and nutritional properties of the bread	260:316	The exopolysaccharides (EPS) produced by LAB affect positively the technological and nutritional properties of the bread, while phytases improve the bioavailability of the minerals by reducing its phytate content.
32702068	3	71	theme	present	418:424	arg1	study					426:430	the present study	414:430	the present study	414:430	In the present study, a pool of 152 cereal-sourced LAB were screened for production of phytases and EPS for potential use as sourdough starter cultures for the baking industry.
32702068	9	72	theme	ad	1745:1746	arg1	medium					1776:1781	an ad hoc formulated barley flour medium	1742:1781	an ad hoc formulated barley flour medium	1742:1781	Furthermore, a few isolates ascribed to L. citreum and W. confusa and carrying the gtf gene produced β-glucans after fermentation in an ad hoc formulated barley flour medium.
32702068	4	73	theme	Sourdough	756:764	arg1	Medium					777:782	Sourdough Simulation Medium	756:782	Sourdough Simulation Medium (SSM)	756:788	There was large heterogeneity in the phytase activity observed among the screened isolates, with 95% showing the ability to degrade sodium phytate on plates containing Sourdough Simulation Medium (SSM).
32702068	4	73	theme	Sourdough	756:764	arg1	SSM					785:787	SSM	785:787	SSM	785:787	There was large heterogeneity in the phytase activity observed among the screened isolates, with 95% showing the ability to degrade sodium phytate on plates containing Sourdough Simulation Medium (SSM).
32702068	2	74	theme	minerals	369:376	arg1	bioavailability					346:360	the bioavailability	342:360	the bioavailability of the minerals	342:376	The exopolysaccharides (EPS) produced by LAB affect positively the technological and nutritional properties of the bread, while phytases improve the bioavailability of the minerals by reducing its phytate content.
32702068	7	75	theme	homopolysaccharides	1395:1413	arg1	production					1367:1376	the production	1363:1376	the production of both HePS and homopolysaccharides (HoPS)	1363:1420	Almost all the EPS producers carried one or more genes (epsD/E and/or epsA) involved in the production of heteropolysaccharides (HePS), whereas the isolates ascribed to Leuconostoc citreum and W. confusa carried genes involved in the production of both HePS and homopolysaccharides (HoPS).
32702068	3	76	theme	baking	571:576	arg1	industry					578:585	the baking industry	567:585	the baking industry	567:585	In the present study, a pool of 152 cereal-sourced LAB were screened for production of phytases and EPS for potential use as sourdough starter cultures for the baking industry.
32702068	10	77	theme	candidate	1836:1844	arg1	cultures					1864:1871	candidate sourdough starter cultures	1836:1871	candidate sourdough starter cultures for the baking industry	1836:1895	Based on the overall results collected, a subset of candidate sourdough starter cultures for the baking industry was selected, including Lb.
32702068	9	78	theme	barley	1763:1768	arg1	medium					1776:1781	an ad hoc formulated barley flour medium	1742:1781	an ad hoc formulated barley flour medium	1742:1781	Furthermore, a few isolates ascribed to L. citreum and W. confusa and carrying the gtf gene produced β-glucans after fermentation in an ad hoc formulated barley flour medium.
32702068	8	79	theme	selected	1484:1491	arg1	subset					1493:1498	a selected subset	1482:1498	a selected subset of isolates	1482:1510	Monosaccharide composition analysis of the EPS produced by a selected subset of isolates revealed that all the HePS included glucose, mannose, and galactose, though at different ratios.
32306780	2	0	dep	composition	550:560	arg1	b					541:541	b	541:541	b	541:541	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	6	1	theme	proteoglycan-4	1381:1394	arg1	concentration					1396:1408	SF proteoglycan-4 concentration	1378:1408	SF proteoglycan-4 concentration	1378:1408	Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test.Results: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis.
32306780	3	2	dep	composition	651:661	arg1	hyaluronan					680:689	hyaluronan	680:689	hyaluronan	680:689	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	3	2	dep	composition	651:661	arg1	proteoglycan-4					664:677	proteoglycan-4	664:677	proteoglycan-4	664:677	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	7	3	theme	serum	1617:1621	arg1	concentrations					1638:1651	SF and serum proteoglycan-4 concentrations	1610:1651	SF and serum proteoglycan-4 concentrations	1610:1651	SF and serum proteoglycan-4 concentrations were correlated in normal horses (r2 = 0.85, p < 0.05), but not in clinical groups.
32306780	3	4	from	samples	856:862	arg1	joints					884:889	various joints	876:889	various joints	876:889	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	7	5	theme	SF	1610:1611	arg1	concentrations					1638:1651	SF and serum proteoglycan-4 concentrations	1610:1651	SF and serum proteoglycan-4 concentrations	1610:1651	SF and serum proteoglycan-4 concentrations were correlated in normal horses (r2 = 0.85, p < 0.05), but not in clinical groups.
32306780	6	6	theme	viscosity	1354:1362	arg1	test.Results					1364:1375	viscosity test.Results	1354:1375	viscosity test.Results	1354:1375	Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test.Results: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis.
32306780	8	7	theme	SF	1888:1889	arg1	Composition					1873:1883	Composition	1873:1883	Composition of SF from cases of equine acute-joint-injury	1873:1929	Cartilage-lubricating ability was unchanged, although steady-shear viscosity of acute-joint-injury SF decreased from normal.Conclusion: Composition of SF from cases of equine acute-joint-injury changed; both proteoglycan-4 concentration and hyaluronan molecular-weight were altered, with decreased SF viscosity, but no associated changes to serum.
32306780	6	8	theme	Biomechanical	1250:1262	arg1	function					1264:1271	Biomechanical function	1250:1271	Biomechanical function of SF	1250:1277	Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test.Results: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis.
32306780	9	9	theme	hyaluronan	2110:2119	arg1	concentrations					2121:2134	hyaluronan concentrations	2110:2134	hyaluronan concentrations	2110:2134	Serum proteoglycan-4 and hyaluronan concentrations alone may not be useful biomarkers for equine joint-injury or disease.
32306780	3	10	from	function	697:704	arg1	horses					734:739	normal horses	727:739	normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods	727:846	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	0	11	theme	cartilage	117:125	arg1	lubrication					136:146	cartilage boundary lubrication	117:146	cartilage boundary lubrication	117:146	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	3	12	dep	groups	762:767	arg1	osteoarthritis					770:783	osteoarthritis	770:783	osteoarthritis	770:783	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	3	12	dep	groups	762:767	arg1	groups					762:767	clinical groups	753:767	clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods	753:846	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	3	12	dep	groups	762:767	arg1	acute-joint-injury					786:803	acute-joint-injury	786:803	acute-joint-injury	786:803	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	3	12	dep	groups	762:767	arg1	osteochondrosis.Materials					810:834	osteochondrosis.Materials	810:834	osteochondrosis.Materials	810:834	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	8	13	theme	equine	1905:1910	arg1	acute-joint-injury					1912:1929	equine acute-joint-injury	1905:1929	equine acute-joint-injury	1905:1929	Cartilage-lubricating ability was unchanged, although steady-shear viscosity of acute-joint-injury SF decreased from normal.Conclusion: Composition of SF from cases of equine acute-joint-injury changed; both proteoglycan-4 concentration and hyaluronan molecular-weight were altered, with decreased SF viscosity, but no associated changes to serum.
32306780	2	14	from	disease	459:465	arg1	composition					489:499	altered SF composition	478:499	altered SF composition	478:499	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	2	14	from	disease	459:465	arg1	function					527:534	diminished mechanical function	505:534	diminished mechanical function	505:534	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	2	14	from	disease	459:465	arg1	composition					550:560	b) serum composition	541:560	b) serum composition	541:560	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	1	15	theme	equine	216:221	arg1	joint-injury					223:234	equine joint-injury	216:234	equine joint-injury	216:234	Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity).
32306780	6	16	theme	cartilage-on-cartilage	1312:1333	arg1	test					1344:1347	an in vitro cartilage-on-cartilage friction test	1300:1347	an in vitro cartilage-on-cartilage friction test	1300:1347	Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test.Results: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis.
32306780	9	17	theme	useful	2153:2158	arg1	biomarkers					2160:2169	useful biomarkers	2153:2169	useful biomarkers for equine joint-injury or disease	2153:2204	Serum proteoglycan-4 and hyaluronan concentrations alone may not be useful biomarkers for equine joint-injury or disease.
32306780	6	18	from	percentage	1519:1528	arg1	osteochondrosis					1593:1607	osteochondrosis	1593:1607	osteochondrosis	1593:1607	Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test.Results: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis.
32306780	6	18	from	percentage	1519:1528	arg1	acute-joint-injury					1570:1587	acute-joint-injury	1570:1587	acute-joint-injury	1570:1587	Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test.Results: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis.
32306780	8	19	theme	associated	2056:2065	arg1	changes					2067:2073	no associated changes	2053:2073	no associated changes to serum	2053:2082	Cartilage-lubricating ability was unchanged, although steady-shear viscosity of acute-joint-injury SF decreased from normal.Conclusion: Composition of SF from cases of equine acute-joint-injury changed; both proteoglycan-4 concentration and hyaluronan molecular-weight were altered, with decreased SF viscosity, but no associated changes to serum.
32306780	6	20	theme	in	1303:1304	arg1	test					1344:1347	an in vitro cartilage-on-cartilage friction test	1300:1347	an in vitro cartilage-on-cartilage friction test	1300:1347	Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test.Results: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis.
32306780	6	21	dep	in	1303:1304	arg1	vitro					1306:1310	vitro	1306:1310	vitro	1306:1310	Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test.Results: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis.
32306780	0	22	theme	synovial	165:172	arg1	fluid					174:178	synovial fluid	165:178	synovial fluid	165:178	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	7	23	theme	clinical	1720:1727	arg1	groups					1729:1734	clinical groups	1720:1734	clinical groups	1720:1734	SF and serum proteoglycan-4 concentrations were correlated in normal horses (r2 = 0.85, p < 0.05), but not in clinical groups.
32306780	2	24	theme	altered	478:484	arg1	composition					489:499	altered SF composition	478:499	altered SF composition	478:499	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	0	25	from	subjects	91:98	arg1	Proteoglycan-4					0:13	Proteoglycan-4	0:13	Proteoglycan-4	0:13	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	0	25	from	subjects	91:98	arg1	composition					30:40	hyaluronan composition	19:40	hyaluronan composition	19:40	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	0	25	from	subjects	91:98	arg1	fluid					54:58	synovial fluid	45:58	synovial fluid	45:58	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	0	25	from	subjects	91:98	arg1	serum					64:68	serum	64:68	serum	64:68	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	3	26	from	joints	884:889	arg1	blood					896:900	blood	896:900	blood	896:900	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	3	26	from	joints	884:889	arg1	SF					867:868	SF	867:868	SF (from various joints)	867:890	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	3	26	from	joints	884:889	arg1	samples					856:862	Equine samples	849:862	Equine samples of SF (from various joints) and blood	849:900	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	6	27	theme	molecular-weight	1539:1554	arg1	hyaluronan					1556:1565	lower molecular-weight hyaluronan	1533:1565	lower molecular-weight hyaluronan	1533:1565	Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test.Results: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis.
32306780	3	28	theme	SF	867:868	arg1	samples					856:862	Equine samples	849:862	Equine samples of SF (from various joints) and blood	849:900	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	3	29	from	horses	734:739	arg1	composition					651:661	composition	651:661	composition (proteoglycan-4, hyaluronan)	651:690	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	3	29	from	horses	734:739	arg1	SF					709:710	SF	709:710	SF	709:710	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	3	29	from	horses	734:739	arg1	serum					716:720	serum	716:720	serum	716:720	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	3	29	from	horses	734:739	arg1	function					697:704	function	697:704	function	697:704	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	0	30	theme	clinical	75:82	arg1	subjects					91:98	clinical equine subjects	75:98	clinical equine subjects	75:98	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	2	31	theme	diminished	505:514	arg1	function					527:534	diminished mechanical function	505:534	diminished mechanical function	505:534	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	0	32	from	Proteoglycan-4	0:13	arg1	serum					64:68	serum	64:68	serum	64:68	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	0	32	from	Proteoglycan-4	0:13	arg1	fluid					54:58	synovial fluid	45:58	synovial fluid	45:58	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	0	32	from	Proteoglycan-4	0:13	arg1	subjects					91:98	clinical equine subjects	75:98	clinical equine subjects	75:98	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	2	33	dep	joint-injury	442:453	arg1	a					423:423	a	423:423	a	423:423	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	2	33	dep	joint-injury	442:453	arg1	hyaluronan					581:590	hyaluronan	581:590	hyaluronan	581:590	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	2	33	dep	joint-injury	442:453	arg1	results					467:473	results	467:473	results	467:473	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	2	33	dep	joint-injury	442:453	arg1	proteoglycan-4					563:576	proteoglycan-4	563:576	proteoglycan-4	563:576	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	1	34	theme	mechanical	351:360	arg1	function					362:369	SF mechanical function	348:369	SF mechanical function (lubrication, viscosity)	348:394	Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity).
32306780	0	35	theme	synovial	45:52	arg1	fluid					54:58	synovial fluid	45:58	synovial fluid	45:58	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	8	36	theme	decreased	2025:2033	arg1	viscosity					2038:2046	decreased SF viscosity	2025:2046	decreased SF viscosity	2025:2046	Cartilage-lubricating ability was unchanged, although steady-shear viscosity of acute-joint-injury SF decreased from normal.Conclusion: Composition of SF from cases of equine acute-joint-injury changed; both proteoglycan-4 concentration and hyaluronan molecular-weight were altered, with decreased SF viscosity, but no associated changes to serum.
32306780	2	37	from	joint-injury	442:453	arg1	composition					489:499	altered SF composition	478:499	altered SF composition	478:499	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	2	37	from	joint-injury	442:453	arg1	function					527:534	diminished mechanical function	505:534	diminished mechanical function	505:534	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	2	37	from	joint-injury	442:453	arg1	composition					550:560	b) serum composition	541:560	b) serum composition	541:560	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	1	38	theme	composition	275:285	arg1	models					206:211	In experimental models	190:211	In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan)	190:314	Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity).
32306780	6	39	theme	normal	1461:1466	arg1	205 ± 106 µg/mL					1468:1482	normal 205 ± 106 µg/mL	1461:1482	normal 205 ± 106 µg/mL	1461:1482	Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test.Results: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis.
32306780	2	40	theme	study	401:405	arg1	changes					593:599	a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes	423:599	a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes	423:599	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	2	40	theme	study	401:405	arg1	hypotheses					407:416	The study hypotheses	397:416	The study hypotheses	397:416	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	0	41	theme	hyaluronan	19:28	arg1	composition					30:40	hyaluronan composition	19:40	hyaluronan composition	19:40	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	5	42	theme	enzyme-linked-immunosorbent-assay	1211:1243	arg1	kit					1245:1247	enzyme-linked-immunosorbent-assay kit	1211:1247	enzyme-linked-immunosorbent-assay kit	1211:1247	Molecular-weight of hyaluronan was characterized by agarose-gel-electrophoresis, and concentrations were measured by enzyme-linked-immunosorbent-assay kit.
32306780	2	43	theme	equine	435:440	arg1	joint-injury					442:453	clinical equine joint-injury	426:453	clinical equine joint-injury	426:453	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	1	44	dep	function	362:369	arg1	viscosity					385:393	viscosity	385:393	viscosity	385:393	Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity).
32306780	1	44	dep	function	362:369	arg1	lubrication					372:382	lubrication	372:382	lubrication	372:382	Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity).
32306780	6	45	theme	increased	1509:1517	arg1	percentage					1519:1528	increased percentage	1509:1528	increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis	1509:1607	Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test.Results: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis.
32306780	8	46	theme	steady-shear	1791:1802	arg1	viscosity					1804:1812	steady-shear viscosity	1791:1812	steady-shear viscosity of acute-joint-injury SF	1791:1837	Cartilage-lubricating ability was unchanged, although steady-shear viscosity of acute-joint-injury SF decreased from normal.Conclusion: Composition of SF from cases of equine acute-joint-injury changed; both proteoglycan-4 concentration and hyaluronan molecular-weight were altered, with decreased SF viscosity, but no associated changes to serum.
32306780	1	47	theme	joint-injury	223:234	arg1	models					206:211	In experimental models	190:211	In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan)	190:314	Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity).
32306780	1	48	dep	experimental	193:204	arg1	In					190:191	In	190:191	In	190:191	Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity).
32306780	1	49	theme	osteoarthritis	240:253	arg1	SF					271:272	SF	271:272	SF	271:272	Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity).
32306780	1	49	theme	osteoarthritis	240:253	arg1	fluid					264:268	osteoarthritis synovial fluid	240:268	osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan)	240:314	Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity).
32306780	7	50	theme	proteoglycan-4	1623:1636	arg1	concentrations					1638:1651	SF and serum proteoglycan-4 concentrations	1610:1651	SF and serum proteoglycan-4 concentrations	1610:1651	SF and serum proteoglycan-4 concentrations were correlated in normal horses (r2 = 0.85, p < 0.05), but not in clinical groups.
32306780	0	51	dep	Proteoglycan-4	0:13	arg1	relationship					101:112	relationship	101:112	relationship to cartilage boundary lubrication	101:146	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	0	51	dep	Proteoglycan-4	0:13	arg1	viscosity					152:160	viscosity	152:160	viscosity of synovial fluid	152:178	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	8	52	theme	Cartilage-lubricating	1737:1757	arg1	ability					1759:1765	Cartilage-lubricating ability	1737:1765	Cartilage-lubricating ability	1737:1765	Cartilage-lubricating ability was unchanged, although steady-shear viscosity of acute-joint-injury SF decreased from normal.Conclusion: Composition of SF from cases of equine acute-joint-injury changed; both proteoglycan-4 concentration and hyaluronan molecular-weight were altered, with decreased SF viscosity, but no associated changes to serum.
32306780	8	53	theme	SF	1836:1837	arg1	viscosity					1804:1812	steady-shear viscosity	1791:1812	steady-shear viscosity of acute-joint-injury SF	1791:1837	Cartilage-lubricating ability was unchanged, although steady-shear viscosity of acute-joint-injury SF decreased from normal.Conclusion: Composition of SF from cases of equine acute-joint-injury changed; both proteoglycan-4 concentration and hyaluronan molecular-weight were altered, with decreased SF viscosity, but no associated changes to serum.
32306780	1	54	theme	fluid	264:268	arg1	composition					275:285	osteoarthritis synovial fluid (SF) composition	240:285	osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan)	240:314	Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity).
32306780	6	55	theme	SF	1378:1379	arg1	concentration					1396:1408	SF proteoglycan-4 concentration	1378:1408	SF proteoglycan-4 concentration	1378:1408	Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test.Results: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis.
32306780	6	56	theme	SF	1276:1277	arg1	function					1264:1271	Biomechanical function	1250:1271	Biomechanical function of SF	1250:1277	Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test.Results: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis.
32306780	9	57	theme	Serum	2085:2089	arg1	proteoglycan-4					2091:2104	Serum proteoglycan-4	2085:2104	Serum proteoglycan-4	2085:2104	Serum proteoglycan-4 and hyaluronan concentrations alone may not be useful biomarkers for equine joint-injury or disease.
32306780	3	58	theme	SF	709:710	arg1	composition					651:661	composition	651:661	composition (proteoglycan-4, hyaluronan)	651:690	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	3	58	theme	SF	709:710	arg1	function					697:704	function	697:704	function	697:704	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	3	59	theme	normal	727:732	arg1	horses					734:739	normal horses	727:739	normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods	727:846	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	0	60	theme	boundary	127:134	arg1	lubrication					136:146	cartilage boundary lubrication	117:146	cartilage boundary lubrication	117:146	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	1	61	theme	experimental	193:204	arg1	models					206:211	In experimental models	190:211	In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan)	190:314	Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity).
32306780	6	62	dep	acute-joint-injury	1423:1440	arg1	1185 ± 276					1443:1452	1185 ± 276	1443:1452	1185 ± 276	1443:1452	Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test.Results: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis.
32306780	6	62	dep	acute-joint-injury	1423:1440	arg1	205 ± 106 µg/mL					1468:1482	normal 205 ± 106 µg/mL	1461:1482	normal 205 ± 106 µg/mL	1461:1482	Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test.Results: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis.
32306780	3	63	theme	serum	716:720	arg1	composition					651:661	composition	651:661	composition (proteoglycan-4, hyaluronan)	651:690	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	3	63	theme	serum	716:720	arg1	function					697:704	function	697:704	function	697:704	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	5	64	theme	hyaluronan	1114:1123	arg1	Molecular-weight					1094:1109	Molecular-weight	1094:1109	Molecular-weight	1094:1109	Molecular-weight of hyaluronan was characterized by agarose-gel-electrophoresis, and concentrations were measured by enzyme-linked-immunosorbent-assay kit.
32306780	6	65	dep	1185 ± 276	1443:1452	arg1	p					1493:1493	p < 0.01	1493:1500	p < 0.01	1493:1500	Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test.Results: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis.
32306780	6	65	dep	1185 ± 276	1443:1452	arg1	µ± SEM					1485:1490	µ± SEM	1485:1490	µ± SEM	1485:1490	Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test.Results: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis.
32306780	2	66	theme	mechanical	516:525	arg1	function					527:534	diminished mechanical function	505:534	diminished mechanical function	505:534	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	6	67	theme	friction	1335:1342	arg1	test					1344:1347	an in vitro cartilage-on-cartilage friction test	1300:1347	an in vitro cartilage-on-cartilage friction test	1300:1347	Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test.Results: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis.
32306780	3	68	theme	clinical	753:760	arg1	osteoarthritis					770:783	osteoarthritis	770:783	osteoarthritis	770:783	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	3	68	theme	clinical	753:760	arg1	groups					762:767	clinical groups	753:767	clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods	753:846	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	3	68	theme	clinical	753:760	arg1	acute-joint-injury					786:803	acute-joint-injury	786:803	acute-joint-injury	786:803	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	8	69	theme	acute-joint-injury	1912:1929	arg1	cases					1896:1900	cases	1896:1900	cases of equine acute-joint-injury	1896:1929	Cartilage-lubricating ability was unchanged, although steady-shear viscosity of acute-joint-injury SF decreased from normal.Conclusion: Composition of SF from cases of equine acute-joint-injury changed; both proteoglycan-4 concentration and hyaluronan molecular-weight were altered, with decreased SF viscosity, but no associated changes to serum.
32306780	2	70	theme	serum	544:548	arg1	composition					550:560	b) serum composition	541:560	b) serum composition	541:560	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	0	71	theme	fluid	174:178	arg1	relationship					101:112	relationship	101:112	relationship to cartilage boundary lubrication	101:146	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	0	71	theme	fluid	174:178	arg1	viscosity					152:160	viscosity	152:160	viscosity of synovial fluid	152:178	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	3	72	theme	Equine	849:854	arg1	samples					856:862	Equine samples	849:862	Equine samples of SF (from various joints) and blood	849:900	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	9	73	theme	equine	2175:2180	arg1	joint-injury					2182:2193	equine joint-injury	2175:2193	equine joint-injury	2175:2193	Serum proteoglycan-4 and hyaluronan concentrations alone may not be useful biomarkers for equine joint-injury or disease.
32306780	8	74	theme	proteoglycan-4	1945:1958	arg1	concentration					1960:1972	proteoglycan-4 concentration	1945:1972	proteoglycan-4 concentration	1945:1972	Cartilage-lubricating ability was unchanged, although steady-shear viscosity of acute-joint-injury SF decreased from normal.Conclusion: Composition of SF from cases of equine acute-joint-injury changed; both proteoglycan-4 concentration and hyaluronan molecular-weight were altered, with decreased SF viscosity, but no associated changes to serum.
32306780	4	75	theme	Proteoglycan-4	939:952	arg1	concentrations					954:967	Proteoglycan-4 concentrations	939:967	Proteoglycan-4 concentrations	939:967	Proteoglycan-4 concentrations were measured by amplified-luminescence-proximity-assay and enzyme-linked-immunosorbent-assay in SF and serum, respectively.
32306780	0	76	from	composition	30:40	arg1	serum					64:68	serum	64:68	serum	64:68	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	0	76	from	composition	30:40	arg1	fluid					54:58	synovial fluid	45:58	synovial fluid	45:58	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	0	76	from	composition	30:40	arg1	subjects					91:98	clinical equine subjects	75:98	clinical equine subjects	75:98	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	7	77	dep	horses	1679:1684	arg1	p					1698:1698	p < 0.05	1698:1705	p < 0.05	1698:1705	SF and serum proteoglycan-4 concentrations were correlated in normal horses (r2 = 0.85, p < 0.05), but not in clinical groups.
32306780	7	77	dep	horses	1679:1684	arg1	r2 = 0.85					1687:1695	r2 = 0.85	1687:1695	r2 = 0.85	1687:1695	SF and serum proteoglycan-4 concentrations were correlated in normal horses (r2 = 0.85, p < 0.05), but not in clinical groups.
32306780	0	78	theme	equine	84:89	arg1	subjects					91:98	clinical equine subjects	75:98	clinical equine subjects	75:98	Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.
32306780	2	79	theme	disease	459:465	arg1	changes					593:599	a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes	423:599	a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes	423:599	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	2	79	theme	disease	459:465	arg1	hypotheses					407:416	The study hypotheses	397:416	The study hypotheses	397:416	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	1	80	dep	composition	275:285	arg1	proteoglycan-4					288:301	proteoglycan-4	288:301	proteoglycan-4	288:301	Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity).
32306780	1	80	dep	composition	275:285	arg1	hyaluronan					304:313	hyaluronan	304:313	hyaluronan	304:313	Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity).
32306780	8	81	theme	molecular-weight	1989:2004	arg1	hyaluronan					1978:1987	hyaluronan molecular-weight	1978:2004	hyaluronan molecular-weight	1978:2004	Cartilage-lubricating ability was unchanged, although steady-shear viscosity of acute-joint-injury SF decreased from normal.Conclusion: Composition of SF from cases of equine acute-joint-injury changed; both proteoglycan-4 concentration and hyaluronan molecular-weight were altered, with decreased SF viscosity, but no associated changes to serum.
32306780	1	82	theme	SF	348:349	arg1	function					362:369	SF mechanical function	348:369	SF mechanical function (lubrication, viscosity)	348:394	Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity).
32306780	2	83	theme	SF	486:487	arg1	composition					489:499	altered SF composition	478:499	altered SF composition	478:499	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	3	84	theme	various	876:882	arg1	joints					884:889	various joints	876:889	various joints	876:889	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	6	85	theme	hyaluronan	1556:1565	arg1	percentage					1519:1528	increased percentage	1509:1528	increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis	1509:1607	Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test.Results: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis.
32306780	8	86	from	cases	1896:1900	arg1	Composition					1873:1883	Composition	1873:1883	Composition of SF from cases of equine acute-joint-injury	1873:1929	Cartilage-lubricating ability was unchanged, although steady-shear viscosity of acute-joint-injury SF decreased from normal.Conclusion: Composition of SF from cases of equine acute-joint-injury changed; both proteoglycan-4 concentration and hyaluronan molecular-weight were altered, with decreased SF viscosity, but no associated changes to serum.
32306780	8	87	theme	SF	2035:2036	arg1	viscosity					2038:2046	decreased SF viscosity	2025:2046	decreased SF viscosity	2025:2046	Cartilage-lubricating ability was unchanged, although steady-shear viscosity of acute-joint-injury SF decreased from normal.Conclusion: Composition of SF from cases of equine acute-joint-injury changed; both proteoglycan-4 concentration and hyaluronan molecular-weight were altered, with decreased SF viscosity, but no associated changes to serum.
32306780	6	88	theme	lower	1533:1537	arg1	hyaluronan					1556:1565	lower molecular-weight hyaluronan	1533:1565	lower molecular-weight hyaluronan	1533:1565	Biomechanical function of SF was characterized by an in vitro cartilage-on-cartilage friction test, and viscosity test.Results: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis.
32306780	3	89	theme	blood	896:900	arg1	samples					856:862	Equine samples	849:862	Equine samples of SF (from various joints) and blood	849:900	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	2	90	theme	joint-injury	442:453	arg1	changes					593:599	a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes	423:599	a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes	423:599	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	2	90	theme	joint-injury	442:453	arg1	hypotheses					407:416	The study hypotheses	397:416	The study hypotheses	397:416	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	2	91	theme	clinical	426:433	arg1	joint-injury					442:453	clinical equine joint-injury	426:453	clinical equine joint-injury	426:453	The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently.
32306780	7	92	theme	normal	1672:1677	arg1	horses					1679:1684	normal horses	1672:1684	normal horses (r2 = 0.85, p < 0.05)	1672:1706	SF and serum proteoglycan-4 concentrations were correlated in normal horses (r2 = 0.85, p < 0.05), but not in clinical groups.
32306780	8	93	theme	acute-joint-injury	1817:1834	arg1	SF					1836:1837	acute-joint-injury SF	1817:1837	acute-joint-injury SF	1817:1837	Cartilage-lubricating ability was unchanged, although steady-shear viscosity of acute-joint-injury SF decreased from normal.Conclusion: Composition of SF from cases of equine acute-joint-injury changed; both proteoglycan-4 concentration and hyaluronan molecular-weight were altered, with decreased SF viscosity, but no associated changes to serum.
32306780	3	94	from	composition	651:661	arg1	horses					734:739	normal horses	727:739	normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods	727:846	The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.Materials and Methods: Equine samples of SF (from various joints) and blood were collected at the point-of-care.
32306780	1	95	theme	synovial	255:262	arg1	SF					271:272	SF	271:272	SF	271:272	Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity).
32306780	1	95	theme	synovial	255:262	arg1	fluid					264:268	osteoarthritis synovial fluid	240:268	osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan)	240:314	Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity).
32289421	7	0	theme	due	1114:1116	arg1	field					1100:1104	the packaging field	1086:1104	the packaging field	1086:1104	According to the above-mentioned experimental results, the g-NCC/PLLA composites can be considered as a potential material in the packaging field, mainly due to its proper biological and physicochemical properties.
32289421	3	1	theme	GPC	391:393	arg1	results					380:386	The results	376:386	The results of GPC	376:393	The results of GPC suggested that the degree of polymerization of the grafted segments was 23, and the degree of hydroxyl substitution of g-NCC was 2.4%.
32289421	6	2	theme	fast	880:883	arg1	method					912:917	a fast and effective modification method	878:917	a fast and effective modification method for g-NCC/PLLA biodegradable composites	878:957	This study provides a fast and effective modification method for g-NCC/PLLA biodegradable composites.
32289421	3	3	theme	polymerization	424:437	arg1	degree					414:419	the degree	410:419	the degree of polymerization of the grafted segments	410:461	The results of GPC suggested that the degree of polymerization of the grafted segments was 23, and the degree of hydroxyl substitution of g-NCC was 2.4%.
32289421	3	3	theme	polymerization	424:437	arg1	23					467:468	23	467:468	23	467:468	The results of GPC suggested that the degree of polymerization of the grafted segments was 23, and the degree of hydroxyl substitution of g-NCC was 2.4%.
32289421	4	4	theme	pure	623:626	arg1	PLLA					628:631	pure PLLA	623:631	pure PLLA	623:631	For the g-NCC/PLLA composite, its crystallization rate increases significantly compared with pure PLLA, indicating that g-NCC acted as a nucleating agent to promote the crystallization.
32289421	0	5	theme	nanocellulose	71:83	arg1	crystal					85:91	nanocellulose crystal	71:91	nanocellulose crystal	71:91	Properties of poly (l-lactic acid) reinforced by l-lactic acid grafted nanocellulose crystal.
32289421	7	6	theme	g-NCC/PLLA	1019:1028	arg1	material					1074:1081	a potential material	1062:1081	a potential material in the packaging field, mainly due to its proper biological and physicochemical properties	1062:1172	According to the above-mentioned experimental results, the g-NCC/PLLA composites can be considered as a potential material in the packaging field, mainly due to its proper biological and physicochemical properties.
32289421	7	6	theme	g-NCC/PLLA	1019:1028	arg1	composites					1030:1039	the g-NCC/PLLA composites	1015:1039	the g-NCC/PLLA composites	1015:1039	According to the above-mentioned experimental results, the g-NCC/PLLA composites can be considered as a potential material in the packaging field, mainly due to its proper biological and physicochemical properties.
32289421	7	7	theme	biological	1132:1141	arg1	properties					1163:1172	its proper biological and physicochemical properties	1121:1172	its proper biological and physicochemical properties	1121:1172	According to the above-mentioned experimental results, the g-NCC/PLLA composites can be considered as a potential material in the packaging field, mainly due to its proper biological and physicochemical properties.
32289421	7	8	theme	packaging	1090:1098	arg1	field					1100:1104	the packaging field	1086:1104	the packaging field	1086:1104	According to the above-mentioned experimental results, the g-NCC/PLLA composites can be considered as a potential material in the packaging field, mainly due to its proper biological and physicochemical properties.
32289421	7	9	theme	physicochemical	1147:1161	arg1	properties					1163:1172	its proper biological and physicochemical properties	1121:1172	its proper biological and physicochemical properties	1121:1172	According to the above-mentioned experimental results, the g-NCC/PLLA composites can be considered as a potential material in the packaging field, mainly due to its proper biological and physicochemical properties.
32289421	6	10	theme	biodegradable	934:946	arg1	composites					948:957	g-NCC/PLLA biodegradable composites	923:957	g-NCC/PLLA biodegradable composites	923:957	This study provides a fast and effective modification method for g-NCC/PLLA biodegradable composites.
32289421	1	11	theme	lactic	153:158	arg1	LA					166:167	LA	166:167	LA	166:167	In this research, nanocellulose crystal (NCC) grafted with lactic acid (LA) oligomer was synthesized by one-pot method and used to reinforce the poly(l-lactic acid) (PLLA) matrix.
32289421	1	11	theme	lactic	153:158	arg1	acid					160:163	lactic acid	153:163	lactic acid (LA) oligomer	153:177	In this research, nanocellulose crystal (NCC) grafted with lactic acid (LA) oligomer was synthesized by one-pot method and used to reinforce the poly(l-lactic acid) (PLLA) matrix.
32289421	5	12	theme	tensile	726:732	arg1	strength					734:741	tensile strength	726:741	tensile strength of the composite materials	726:768	Moreover, tensile strength of the composite materials was significantly improved from 41.9 MPa to 53.9 MPa due to the enhanced compatibility.
32289421	2	13	theme	nanocellulose	345:357	arg1	g-NCC					368:372	g-NCC	368:372	g-NCC	368:372	FT-IR, XRD, and 13C NMR were used to analyze the structure of modified nanocellulose crystal (g-NCC).
32289421	2	13	theme	nanocellulose	345:357	arg1	crystal					359:365	modified nanocellulose crystal	336:365	modified nanocellulose crystal (g-NCC)	336:373	FT-IR, XRD, and 13C NMR were used to analyze the structure of modified nanocellulose crystal (g-NCC).
32289421	7	14	theme	above-mentioned	977:991	arg1	results					1006:1012	the above-mentioned experimental results	973:1012	the above-mentioned experimental results	973:1012	According to the above-mentioned experimental results, the g-NCC/PLLA composites can be considered as a potential material in the packaging field, mainly due to its proper biological and physicochemical properties.
32289421	3	15	theme	segments	454:461	arg1	polymerization					424:437	polymerization	424:437	polymerization of the grafted segments	424:461	The results of GPC suggested that the degree of polymerization of the grafted segments was 23, and the degree of hydroxyl substitution of g-NCC was 2.4%.
32289421	7	16	from	material	1074:1081	arg1	field					1100:1104	the packaging field	1086:1104	the packaging field	1086:1104	According to the above-mentioned experimental results, the g-NCC/PLLA composites can be considered as a potential material in the packaging field, mainly due to its proper biological and physicochemical properties.
32289421	2	17	theme	13C	290:292	arg1	NMR					294:296	13C NMR	290:296	13C NMR	290:296	FT-IR, XRD, and 13C NMR were used to analyze the structure of modified nanocellulose crystal (g-NCC).
32289421	2	18	theme	modified	336:343	arg1	g-NCC					368:372	g-NCC	368:372	g-NCC	368:372	FT-IR, XRD, and 13C NMR were used to analyze the structure of modified nanocellulose crystal (g-NCC).
32289421	2	18	theme	modified	336:343	arg1	crystal					359:365	modified nanocellulose crystal	336:365	modified nanocellulose crystal (g-NCC)	336:373	FT-IR, XRD, and 13C NMR were used to analyze the structure of modified nanocellulose crystal (g-NCC).
32289421	7	19	theme	experimental	993:1004	arg1	results					1006:1012	the above-mentioned experimental results	973:1012	the above-mentioned experimental results	973:1012	According to the above-mentioned experimental results, the g-NCC/PLLA composites can be considered as a potential material in the packaging field, mainly due to its proper biological and physicochemical properties.
32289421	5	20	theme	due	823:825	arg1	53.9 MPa					814:821	53.9 MPa	814:821	53.9 MPa due to the enhanced compatibility	814:855	Moreover, tensile strength of the composite materials was significantly improved from 41.9 MPa to 53.9 MPa due to the enhanced compatibility.
32289421	1	21	theme	acid	160:163	arg1	oligomer					170:177	lactic acid (LA) oligomer	153:177	lactic acid (LA) oligomer	153:177	In this research, nanocellulose crystal (NCC) grafted with lactic acid (LA) oligomer was synthesized by one-pot method and used to reinforce the poly(l-lactic acid) (PLLA) matrix.
32289421	7	22	theme	potential	1064:1072	arg1	material					1074:1081	a potential material	1062:1081	a potential material in the packaging field, mainly due to its proper biological and physicochemical properties	1062:1172	According to the above-mentioned experimental results, the g-NCC/PLLA composites can be considered as a potential material in the packaging field, mainly due to its proper biological and physicochemical properties.
32289421	7	22	theme	potential	1064:1072	arg1	composites					1030:1039	the g-NCC/PLLA composites	1015:1039	the g-NCC/PLLA composites	1015:1039	According to the above-mentioned experimental results, the g-NCC/PLLA composites can be considered as a potential material in the packaging field, mainly due to its proper biological and physicochemical properties.
32289421	5	23	theme	enhanced	834:841	arg1	compatibility					843:855	the enhanced compatibility	830:855	the enhanced compatibility	830:855	Moreover, tensile strength of the composite materials was significantly improved from 41.9 MPa to 53.9 MPa due to the enhanced compatibility.
32289421	0	24	theme	poly	14:17	arg1	Properties					0:9	Properties	0:9	Properties of poly (l-lactic acid)	0:33	Properties of poly (l-lactic acid) reinforced by l-lactic acid grafted nanocellulose crystal.
32289421	1	25	theme	poly	239:242	arg1	matrix					266:271	the poly(l-lactic acid) (PLLA) matrix	235:271	the poly(l-lactic acid) (PLLA) matrix	235:271	In this research, nanocellulose crystal (NCC) grafted with lactic acid (LA) oligomer was synthesized by one-pot method and used to reinforce the poly(l-lactic acid) (PLLA) matrix.
32289421	3	26	theme	grafted	446:452	arg1	segments					454:461	the grafted segments	442:461	the grafted segments	442:461	The results of GPC suggested that the degree of polymerization of the grafted segments was 23, and the degree of hydroxyl substitution of g-NCC was 2.4%.
32289421	5	27	theme	composite	750:758	arg1	materials					760:768	the composite materials	746:768	the composite materials	746:768	Moreover, tensile strength of the composite materials was significantly improved from 41.9 MPa to 53.9 MPa due to the enhanced compatibility.
32289421	1	28	used	used	217:220	arg2	NCC					135:137	nanocellulose crystal (NCC)	112:138	nanocellulose crystal (NCC) grafted with lactic acid (LA) oligomer	112:177	In this research, nanocellulose crystal (NCC) grafted with lactic acid (LA) oligomer was synthesized by one-pot method and used to reinforce the poly(l-lactic acid) (PLLA) matrix.
32289421	6	29	theme	g-NCC/PLLA	923:932	arg1	composites					948:957	g-NCC/PLLA biodegradable composites	923:957	g-NCC/PLLA biodegradable composites	923:957	This study provides a fast and effective modification method for g-NCC/PLLA biodegradable composites.
32289421	0	30	theme	l-lactic	20:27	arg1	poly					14:17	poly	14:17	poly (l-lactic acid)	14:33	Properties of poly (l-lactic acid) reinforced by l-lactic acid grafted nanocellulose crystal.
32289421	0	30	theme	l-lactic	20:27	arg1	acid					29:32	l-lactic acid	20:32	l-lactic acid	20:32	Properties of poly (l-lactic acid) reinforced by l-lactic acid grafted nanocellulose crystal.
32289421	1	31	theme	l-lactic	244:251	arg1	poly					239:242	poly	239:242	the poly(l-lactic acid) (PLLA) matrix	235:271	In this research, nanocellulose crystal (NCC) grafted with lactic acid (LA) oligomer was synthesized by one-pot method and used to reinforce the poly(l-lactic acid) (PLLA) matrix.
32289421	1	31	theme	l-lactic	244:251	arg1	acid					253:256	l-lactic acid	244:256	l-lactic acid	244:256	In this research, nanocellulose crystal (NCC) grafted with lactic acid (LA) oligomer was synthesized by one-pot method and used to reinforce the poly(l-lactic acid) (PLLA) matrix.
32289421	2	32	used	used	303:306	arg2	XRD					281:283	XRD	281:283	XRD	281:283	FT-IR, XRD, and 13C NMR were used to analyze the structure of modified nanocellulose crystal (g-NCC).
32289421	2	32	used	used	303:306	arg2	FT-IR					274:278	FT-IR	274:278	FT-IR	274:278	FT-IR, XRD, and 13C NMR were used to analyze the structure of modified nanocellulose crystal (g-NCC).
32289421	2	32	used	used	303:306	arg2	NMR					294:296	13C NMR	290:296	13C NMR	290:296	FT-IR, XRD, and 13C NMR were used to analyze the structure of modified nanocellulose crystal (g-NCC).
32289421	3	33	theme	g-NCC	514:518	arg1	substitution					498:509	hydroxyl substitution	489:509	hydroxyl substitution of g-NCC	489:518	The results of GPC suggested that the degree of polymerization of the grafted segments was 23, and the degree of hydroxyl substitution of g-NCC was 2.4%.
32289421	2	34	theme	crystal	359:365	arg1	structure					323:331	the structure	319:331	the structure of modified nanocellulose crystal (g-NCC)	319:373	FT-IR, XRD, and 13C NMR were used to analyze the structure of modified nanocellulose crystal (g-NCC).
32289421	4	35	theme	crystallization	564:578	arg1	rate					580:583	its crystallization rate	560:583	its crystallization rate	560:583	For the g-NCC/PLLA composite, its crystallization rate increases significantly compared with pure PLLA, indicating that g-NCC acted as a nucleating agent to promote the crystallization.
32289421	3	36	theme	hydroxyl	489:496	arg1	substitution					498:509	hydroxyl substitution	489:509	hydroxyl substitution of g-NCC	489:518	The results of GPC suggested that the degree of polymerization of the grafted segments was 23, and the degree of hydroxyl substitution of g-NCC was 2.4%.
32289421	3	37	theme	substitution	498:509	arg1	%					527:527	2.4%	524:527	2.4%	524:527	The results of GPC suggested that the degree of polymerization of the grafted segments was 23, and the degree of hydroxyl substitution of g-NCC was 2.4%.
32289421	3	37	theme	substitution	498:509	arg1	degree					479:484	the degree	475:484	the degree of hydroxyl substitution of g-NCC	475:518	The results of GPC suggested that the degree of polymerization of the grafted segments was 23, and the degree of hydroxyl substitution of g-NCC was 2.4%.
32289421	1	38	theme	nanocellulose	112:124	arg1	NCC					135:137	nanocellulose crystal (NCC)	112:138	nanocellulose crystal (NCC) grafted with lactic acid (LA) oligomer	112:177	In this research, nanocellulose crystal (NCC) grafted with lactic acid (LA) oligomer was synthesized by one-pot method and used to reinforce the poly(l-lactic acid) (PLLA) matrix.
32289421	6	39	theme	modification	899:910	arg1	method					912:917	a fast and effective modification method	878:917	a fast and effective modification method for g-NCC/PLLA biodegradable composites	878:957	This study provides a fast and effective modification method for g-NCC/PLLA biodegradable composites.
32289421	0	40	theme	l-lactic	49:56	arg1	acid					58:61	l-lactic acid	49:61	l-lactic acid grafted nanocellulose crystal	49:91	Properties of poly (l-lactic acid) reinforced by l-lactic acid grafted nanocellulose crystal.
32289421	5	41	theme	materials	760:768	arg1	strength					734:741	tensile strength	726:741	tensile strength of the composite materials	726:768	Moreover, tensile strength of the composite materials was significantly improved from 41.9 MPa to 53.9 MPa due to the enhanced compatibility.
32289421	4	42	theme	nucleating	667:676	arg1	agent					678:682	a nucleating agent	665:682	a nucleating agent to promote the crystallization	665:713	For the g-NCC/PLLA composite, its crystallization rate increases significantly compared with pure PLLA, indicating that g-NCC acted as a nucleating agent to promote the crystallization.
32289421	4	42	theme	nucleating	667:676	arg1	g-NCC					650:654	g-NCC	650:654	g-NCC	650:654	For the g-NCC/PLLA composite, its crystallization rate increases significantly compared with pure PLLA, indicating that g-NCC acted as a nucleating agent to promote the crystallization.
32289421	1	43	theme	crystal	126:132	arg1	NCC					135:137	nanocellulose crystal (NCC)	112:138	nanocellulose crystal (NCC) grafted with lactic acid (LA) oligomer	112:177	In this research, nanocellulose crystal (NCC) grafted with lactic acid (LA) oligomer was synthesized by one-pot method and used to reinforce the poly(l-lactic acid) (PLLA) matrix.
32289421	6	44	theme	effective	889:897	arg1	method					912:917	a fast and effective modification method	878:917	a fast and effective modification method for g-NCC/PLLA biodegradable composites	878:957	This study provides a fast and effective modification method for g-NCC/PLLA biodegradable composites.
32289421	7	45	theme	proper	1125:1130	arg1	properties					1163:1172	its proper biological and physicochemical properties	1121:1172	its proper biological and physicochemical properties	1121:1172	According to the above-mentioned experimental results, the g-NCC/PLLA composites can be considered as a potential material in the packaging field, mainly due to its proper biological and physicochemical properties.
32289421	4	46	theme	g-NCC/PLLA	538:547	arg1	composite					549:557	the g-NCC/PLLA composite	534:557	the g-NCC/PLLA composite	534:557	For the g-NCC/PLLA composite, its crystallization rate increases significantly compared with pure PLLA, indicating that g-NCC acted as a nucleating agent to promote the crystallization.
32289421	1	47	theme	one-pot	198:204	arg1	method					206:211	one-pot method	198:211	one-pot method	198:211	In this research, nanocellulose crystal (NCC) grafted with lactic acid (LA) oligomer was synthesized by one-pot method and used to reinforce the poly(l-lactic acid) (PLLA) matrix.
32001283	3	0	dep	functional	401:410	arg1	rheological					413:423	rheological	413:423	rheological	413:423	In this study we described the functional (rheological, mechanical, surface, structural) properties of gels and foils containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry.
32001283	3	0	dep	functional	401:410	arg1	structural					447:456	structural	447:456	structural	447:456	In this study we described the functional (rheological, mechanical, surface, structural) properties of gels and foils containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry.
32001283	3	0	dep	functional	401:410	arg1	surface					438:444	surface	438:444	surface	438:444	In this study we described the functional (rheological, mechanical, surface, structural) properties of gels and foils containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry.
32001283	3	0	dep	functional	401:410	arg1	mechanical					426:435	mechanical	426:435	mechanical	426:435	In this study we described the functional (rheological, mechanical, surface, structural) properties of gels and foils containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry.
32001283	3	1	theme	hyaluronan	532:541	arg1	matrix					567:572	hyaluronan and hyaluronan-lecithin matrix	532:572	hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry	532:618	In this study we described the functional (rheological, mechanical, surface, structural) properties of gels and foils containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry.
32001283	6	2	theme	materials	1025:1033	arg1	adaptation					994:1003	the adaptation	990:1003	the adaptation of the investigated materials to their potential applications	990:1065	The results of the study are significant for the adaptation of the investigated materials to their potential applications.
32001283	1	3	theme	nanomedicine	188:199	arg1	development					173:183	the development	169:183	the development of nanomedicine	169:199	The rapid progress of nanotechnology triggers the development of nanomedicine.
32001283	0	4	from	hyaluronan-lecithin	95:113	arg1	embedded					68:75	embedded	68:75	embedded	68:75	Functional properties of composites containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix.
32001283	3	5	theme	green	604:608	arg1	chemistry					610:618	green chemistry	604:618	green chemistry	604:618	In this study we described the functional (rheological, mechanical, surface, structural) properties of gels and foils containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry.
32001283	3	6	theme	chemistry	610:618	arg1	methods					593:599	the methods	589:599	the methods of green chemistry	589:618	In this study we described the functional (rheological, mechanical, surface, structural) properties of gels and foils containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry.
32001283	0	7	from	embedded	68:75	arg1	hyaluronan-lecithin					95:113	hyaluronan-lecithin	95:113	hyaluronan-lecithin	95:113	Functional properties of composites containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix.
32001283	0	7	from	embedded	68:75	arg1	hyaluronan					80:89	hyaluronan	80:89	hyaluronan	80:89	Functional properties of composites containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix.
32001283	0	8	contain	containing	36:45	arg2	silver					47:52	silver	47:52	silver	47:52	Functional properties of composites containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix.
32001283	0	8	contain	containing	36:45	arg1	composites					25:34	composites	25:34	composites containing silver	25:52	Functional properties of composites containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix.
32001283	4	9	theme	sample	738:743	arg1	stretch					723:729	the stretch	719:729	the stretch of the sample	719:743	The study showed that the addition of silver strengthened the structure of Hyal foil, but reduced the stretch of the sample and that lecithin weakened the mechanical properties of the composites.
32001283	5	10	theme	studied	859:865	arg1	foils					867:871	the studied foils	855:871	the studied foils partially hydrophilic	855:893	Also, the presence of nanosilver made the studied foils partially hydrophilic, while these with lecithin were more hydrophobic.
32001283	3	11	from	matrix	567:572	arg1	embedded					520:527	embedded	520:527	embedded	520:527	In this study we described the functional (rheological, mechanical, surface, structural) properties of gels and foils containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry.
32001283	4	12	theme	composites	805:814	arg1	properties					787:796	the mechanical properties	772:796	the mechanical properties of the composites	772:814	The study showed that the addition of silver strengthened the structure of Hyal foil, but reduced the stretch of the sample and that lecithin weakened the mechanical properties of the composites.
32001283	4	13	theme	foil	701:704	arg1	structure					683:691	the structure	679:691	the structure of Hyal foil	679:704	The study showed that the addition of silver strengthened the structure of Hyal foil, but reduced the stretch of the sample and that lecithin weakened the mechanical properties of the composites.
32001283	6	14	theme	potential	1044:1052	arg1	applications					1054:1065	their potential applications	1038:1065	their potential applications	1038:1065	The results of the study are significant for the adaptation of the investigated materials to their potential applications.
32001283	4	15	theme	Hyal	696:699	arg1	foil					701:704	Hyal foil	696:704	Hyal foil	696:704	The study showed that the addition of silver strengthened the structure of Hyal foil, but reduced the stretch of the sample and that lecithin weakened the mechanical properties of the composites.
32001283	2	16	theme	environmentally-friendly	336:359	arg1	methods					361:367	environmentally-friendly methods	336:367	environmentally-friendly methods	336:367	As the antimicrobial properties of nanosilver are well known, there is a huge interest in the synthesis of silver nanoparticles using environmentally-friendly methods.
32001283	0	17	theme	Functional	0:9	arg1	properties					11:20	Functional properties	0:20	Functional properties of composites containing silver	0:52	Functional properties of composites containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix.
32001283	3	18	from	embedded	520:527	arg1	matrix					567:572	hyaluronan and hyaluronan-lecithin matrix	532:572	hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry	532:618	In this study we described the functional (rheological, mechanical, surface, structural) properties of gels and foils containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry.
32001283	0	19	theme	composites	25:34	arg1	properties					11:20	Functional properties	0:20	Functional properties of composites containing silver	0:52	Functional properties of composites containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix.
32001283	2	20	theme	nanoparticles	316:328	arg1	synthesis					296:304	the synthesis	292:304	the synthesis of silver nanoparticles using environmentally-friendly methods	292:367	As the antimicrobial properties of nanosilver are well known, there is a huge interest in the synthesis of silver nanoparticles using environmentally-friendly methods.
32001283	3	21	theme	foils	482:486	arg1	properties					459:468	the functional (rheological, mechanical, surface, structural) properties	397:468	the functional (rheological, mechanical, surface, structural) properties of gels and foils containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry	397:618	In this study we described the functional (rheological, mechanical, surface, structural) properties of gels and foils containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry.
32001283	2	22	theme	antimicrobial	209:221	arg1	properties					223:232	the antimicrobial properties	205:232	the antimicrobial properties of nanosilver	205:246	As the antimicrobial properties of nanosilver are well known, there is a huge interest in the synthesis of silver nanoparticles using environmentally-friendly methods.
32001283	1	23	theme	rapid	127:131	arg1	progress					133:140	The rapid progress	123:140	The rapid progress of nanotechnology	123:158	The rapid progress of nanotechnology triggers the development of nanomedicine.
32001283	2	24	from	interest	280:287	arg1	synthesis					296:304	the synthesis	292:304	the synthesis of silver nanoparticles using environmentally-friendly methods	292:367	As the antimicrobial properties of nanosilver are well known, there is a huge interest in the synthesis of silver nanoparticles using environmentally-friendly methods.
32001283	5	25	theme	nanosilver	839:848	arg1	presence					827:834	the presence	823:834	the presence of nanosilver	823:848	Also, the presence of nanosilver made the studied foils partially hydrophilic, while these with lecithin were more hydrophobic.
32001283	3	26	theme	gels	473:476	arg1	properties					459:468	the functional (rheological, mechanical, surface, structural) properties	397:468	the functional (rheological, mechanical, surface, structural) properties of gels and foils containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry	397:618	In this study we described the functional (rheological, mechanical, surface, structural) properties of gels and foils containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry.
32001283	5	27	theme	hydrophilic	883:893	arg1	foils					867:871	the studied foils	855:871	the studied foils partially hydrophilic	855:893	Also, the presence of nanosilver made the studied foils partially hydrophilic, while these with lecithin were more hydrophobic.
32001283	6	28	theme	investigated	1012:1023	arg1	materials					1025:1033	the investigated materials	1008:1033	the investigated materials	1008:1033	The results of the study are significant for the adaptation of the investigated materials to their potential applications.
32001283	3	29	contain	containing	488:497	arg2	nanoparticles					506:518	silver nanoparticles	499:518	silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry	499:618	In this study we described the functional (rheological, mechanical, surface, structural) properties of gels and foils containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry.
32001283	3	29	contain	containing	488:497	arg1	gels					473:476	gels	473:476	gels	473:476	In this study we described the functional (rheological, mechanical, surface, structural) properties of gels and foils containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry.
32001283	3	30	theme	functional	401:410	arg1	properties					459:468	the functional (rheological, mechanical, surface, structural) properties	397:468	the functional (rheological, mechanical, surface, structural) properties of gels and foils containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry	397:618	In this study we described the functional (rheological, mechanical, surface, structural) properties of gels and foils containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry.
32001283	4	31	theme	mechanical	776:785	arg1	properties					787:796	the mechanical properties	772:796	the mechanical properties of the composites	772:814	The study showed that the addition of silver strengthened the structure of Hyal foil, but reduced the stretch of the sample and that lecithin weakened the mechanical properties of the composites.
32001283	6	32	theme	study	964:968	arg1	significant					974:984	significant	974:984	significant	974:984	The results of the study are significant for the adaptation of the investigated materials to their potential applications.
32001283	6	32	theme	study	964:968	arg1	results					949:955	The results	945:955	The results of the study	945:968	The results of the study are significant for the adaptation of the investigated materials to their potential applications.
32001283	3	33	theme	embedded	520:527	arg1	nanoparticles					506:518	silver nanoparticles	499:518	silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry	499:618	In this study we described the functional (rheological, mechanical, surface, structural) properties of gels and foils containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry.
32001283	0	34	from	hyaluronan	80:89	arg1	embedded					68:75	embedded	68:75	embedded	68:75	Functional properties of composites containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix.
32001283	2	35	theme	silver	309:314	arg1	nanoparticles					316:328	silver nanoparticles	309:328	silver nanoparticles using environmentally-friendly methods	309:367	As the antimicrobial properties of nanosilver are well known, there is a huge interest in the synthesis of silver nanoparticles using environmentally-friendly methods.
32001283	2	36	theme	nanosilver	237:246	arg1	properties					223:232	the antimicrobial properties	205:232	the antimicrobial properties of nanosilver	205:246	As the antimicrobial properties of nanosilver are well known, there is a huge interest in the synthesis of silver nanoparticles using environmentally-friendly methods.
32001283	1	37	theme	nanotechnology	145:158	arg1	progress					133:140	The rapid progress	123:140	The rapid progress of nanotechnology	123:158	The rapid progress of nanotechnology triggers the development of nanomedicine.
32001283	0	38	dep	hyaluronan	80:89	arg1	matrix					115:120	matrix	115:120	matrix	115:120	Functional properties of composites containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix.
32001283	2	39	theme	huge	275:278	arg1	interest					280:287	a huge interest	273:287	a huge interest in the synthesis of silver nanoparticles using environmentally-friendly methods	273:367	As the antimicrobial properties of nanosilver are well known, there is a huge interest in the synthesis of silver nanoparticles using environmentally-friendly methods.
32001283	3	40	theme	silver	499:504	arg1	nanoparticles					506:518	silver nanoparticles	499:518	silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry	499:618	In this study we described the functional (rheological, mechanical, surface, structural) properties of gels and foils containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry.
32001283	3	41	theme	hyaluronan-lecithin	547:565	arg1	matrix					567:572	hyaluronan and hyaluronan-lecithin matrix	532:572	hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry	532:618	In this study we described the functional (rheological, mechanical, surface, structural) properties of gels and foils containing silver nanoparticles embedded in hyaluronan and hyaluronan-lecithin matrix prepared using the methods of green chemistry.
32001283	4	42	theme	silver	659:664	arg1	addition					647:654	the addition	643:654	the addition of silver	643:664	The study showed that the addition of silver strengthened the structure of Hyal foil, but reduced the stretch of the sample and that lecithin weakened the mechanical properties of the composites.
33314139	10	0	theme	long-chain	1444:1453	arg1	DP					1468:1469	DP 35-60	1468:1475	DP 35-60	1468:1475	By contrast, long branch-chain amylopectin, long-chain amylopectin (DP 35-60), glutelin and prolamin had adverse effects on eating quality of rice.
33314139	10	0	theme	long-chain	1444:1453	arg1	amylopectin					1455:1465	long-chain amylopectin	1444:1465	long-chain amylopectin (DP 35-60)	1444:1476	By contrast, long branch-chain amylopectin, long-chain amylopectin (DP 35-60), glutelin and prolamin had adverse effects on eating quality of rice.
33314139	0	1	with	quality	70:76	arg1	contents					117:124	similar amylose contents	101:124	similar amylose contents	101:124	Influence of starch fine structure and storage proteins on the eating quality of rice varieties with similar amylose contents.
33314139	1	2	theme	excellent	239:247	arg1	quality					256:262	excellent eating quality	239:262	excellent eating quality	239:262	BACKGROUND Rice eating quality largely dictates consumer preference, and the demand for new rice varieties with excellent eating quality from farmers is increasing.
33314139	6	3	theme	eating	847:852	arg1	quality					854:860	high eating quality	842:860	high eating quality	842:860	We found that, in both groups, the rice varieties with high eating quality had more short-chain amylopectin, lower glutelin and prolamin content, and a higher albumin content.
33314139	3	4	with	groups	416:421	arg1	content					463:469	different apparent amylose content	436:469	different apparent amylose content (AACs)	436:476	RESULTS Two groups of rice with different apparent amylose content (AACs) were used in this study.
33314139	3	4	with	groups	416:421	arg1	AACs					472:475	AACs	472:475	AACs	472:475	RESULTS Two groups of rice with different apparent amylose content (AACs) were used in this study.
33314139	2	5	theme	rice	388:391	arg1	varieties					393:401	high-quality rice varieties	375:401	high-quality rice varieties	375:401	Identification of factors contributing to eating quality is helpful for developing high-quality rice varieties.
33314139	4	6	contain	contained	513:521	arg1	group					507:511	One group	503:511	One group	503:511	One group contained four varieties with low AACs (8.8-9.4%), whereas the other contained four traditional varieties with medium AACs (17.2-17.5%).
33314139	4	6	contain	contained	513:521	arg2	varieties					528:536	four varieties	523:536	four varieties with low AACs (8.8-9.4%)	523:561	One group contained four varieties with low AACs (8.8-9.4%), whereas the other contained four traditional varieties with medium AACs (17.2-17.5%).
33314139	6	7	theme	rice	822:825	arg1	varieties					827:835	the rice varieties	818:835	the rice varieties with high eating quality	818:860	We found that, in both groups, the rice varieties with high eating quality had more short-chain amylopectin, lower glutelin and prolamin content, and a higher albumin content.
33314139	8	8	theme	protein	1173:1179	arg1	composition					1181:1191	the storage protein composition	1161:1191	the storage protein composition	1161:1191	CONCLUSIONS Both the fine structure of starch and the storage protein composition were closely related to rice eating quality.
33314139	1	9	from	farmers	269:275	arg1	varieties					224:232	new rice varieties	215:232	new rice varieties with excellent eating quality from farmers	215:275	BACKGROUND Rice eating quality largely dictates consumer preference, and the demand for new rice varieties with excellent eating quality from farmers is increasing.
33314139	9	10	theme	positive	1364:1371	arg1	effects					1373:1379	positive effects	1364:1379	positive effects	1364:1379	In both groups, short branch-chain amylopectin, short-chain amylopectin [degree of polymerization (DP) 6-12], and albumin had positive effects on eating quality.
33314139	6	11	theme	prolamin	915:922	arg1	content					924:930	lower glutelin and prolamin content	896:930	lower glutelin and prolamin content	896:930	We found that, in both groups, the rice varieties with high eating quality had more short-chain amylopectin, lower glutelin and prolamin content, and a higher albumin content.
33314139	4	12	theme	traditional	597:607	arg1	varieties					609:617	four traditional varieties	592:617	four traditional varieties with medium AACs (17.2-17.5%)	592:647	One group contained four varieties with low AACs (8.8-9.4%), whereas the other contained four traditional varieties with medium AACs (17.2-17.5%).
33314139	0	13	theme	similar	101:107	arg1	contents					117:124	similar amylose contents	101:124	similar amylose contents	101:124	Influence of starch fine structure and storage proteins on the eating quality of rice varieties with similar amylose contents.
33314139	6	14	theme	glutelin	902:909	arg1	content					924:930	lower glutelin and prolamin content	896:930	lower glutelin and prolamin content	896:930	We found that, in both groups, the rice varieties with high eating quality had more short-chain amylopectin, lower glutelin and prolamin content, and a higher albumin content.
33314139	10	15	theme	eating	1524:1529	arg1	quality					1531:1537	eating quality	1524:1537	eating quality of rice	1524:1545	By contrast, long branch-chain amylopectin, long-chain amylopectin (DP 35-60), glutelin and prolamin had adverse effects on eating quality of rice.
33314139	5	16	theme	starch	682:687	arg1	structure					694:702	starch fine structure	682:702	starch fine structure	682:702	The physicochemical properties, starch fine structure and crystallinity and storage protein composition of the two groups were analyzed.
33314139	8	17	theme	starch	1150:1155	arg1	structure					1137:1145	the fine structure	1128:1145	the fine structure of starch	1128:1155	CONCLUSIONS Both the fine structure of starch and the storage protein composition were closely related to rice eating quality.
33314139	8	17	theme	starch	1150:1155	arg1	composition					1181:1191	the storage protein composition	1161:1191	the storage protein composition	1161:1191	CONCLUSIONS Both the fine structure of starch and the storage protein composition were closely related to rice eating quality.
33314139	8	18	theme	rice	1217:1220	arg1	quality					1229:1235	rice eating quality	1217:1235	rice eating quality	1217:1235	CONCLUSIONS Both the fine structure of starch and the storage protein composition were closely related to rice eating quality.
33314139	7	19	theme	low-AAC	980:986	arg1	varieties					988:996	the low-AAC varieties	976:996	the low-AAC varieties	976:996	In addition, the low-AAC varieties produced opaque endosperms, which may result from an increased number of pores in the center of starch granules.
33314139	6	20	theme	short-chain	871:881	arg1	amylopectin					883:893	more short-chain amylopectin	866:893	more short-chain amylopectin	866:893	We found that, in both groups, the rice varieties with high eating quality had more short-chain amylopectin, lower glutelin and prolamin content, and a higher albumin content.
33314139	7	21	theme	pores	1071:1075	arg1	number					1061:1066	an increased number	1048:1066	an increased number of pores in the center of starch granules	1048:1108	In addition, the low-AAC varieties produced opaque endosperms, which may result from an increased number of pores in the center of starch granules.
33314139	4	22	contain	contained	582:590	arg1	other					576:580	other	576:580	other	576:580	One group contained four varieties with low AACs (8.8-9.4%), whereas the other contained four traditional varieties with medium AACs (17.2-17.5%).
33314139	4	22	contain	contained	582:590	arg2	varieties					609:617	four traditional varieties	592:617	four traditional varieties with medium AACs (17.2-17.5%)	592:647	One group contained four varieties with low AACs (8.8-9.4%), whereas the other contained four traditional varieties with medium AACs (17.2-17.5%).
33314139	5	23	theme	storage	726:732	arg1	composition					742:752	storage protein composition	726:752	storage protein composition	726:752	The physicochemical properties, starch fine structure and crystallinity and storage protein composition of the two groups were analyzed.
33314139	1	24	theme	Rice	138:141	arg1	quality					150:156	BACKGROUND Rice eating quality	127:156	BACKGROUND Rice eating quality	127:156	BACKGROUND Rice eating quality largely dictates consumer preference, and the demand for new rice varieties with excellent eating quality from farmers is increasing.
33314139	6	25	theme	albumin	946:952	arg1	content					954:960	a higher albumin content	937:960	a higher albumin content	937:960	We found that, in both groups, the rice varieties with high eating quality had more short-chain amylopectin, lower glutelin and prolamin content, and a higher albumin content.
33314139	7	26	from	pores	1071:1075	arg1	center					1084:1089	the center	1080:1089	the center of starch granules	1080:1108	In addition, the low-AAC varieties produced opaque endosperms, which may result from an increased number of pores in the center of starch granules.
33314139	0	27	theme	eating	63:68	arg1	quality					70:76	the eating quality	59:76	the eating quality of rice varieties with similar amylose contents	59:124	Influence of starch fine structure and storage proteins on the eating quality of rice varieties with similar amylose contents.
33314139	4	28	dep	contained	513:521	arg1	whereas					564:570	whereas	564:570	whereas	564:570	One group contained four varieties with low AACs (8.8-9.4%), whereas the other contained four traditional varieties with medium AACs (17.2-17.5%).
33314139	0	29	from	Influence	0:8	arg1	quality					70:76	the eating quality	59:76	the eating quality of rice varieties with similar amylose contents	59:124	Influence of starch fine structure and storage proteins on the eating quality of rice varieties with similar amylose contents.
33314139	3	30	dep	RESULTS	404:410	arg1	used					483:486	used	483:486	were used in this study	478:500	RESULTS Two groups of rice with different apparent amylose content (AACs) were used in this study.
33314139	3	31	theme	different	436:444	arg1	content					463:469	different apparent amylose content	436:469	different apparent amylose content (AACs)	436:476	RESULTS Two groups of rice with different apparent amylose content (AACs) were used in this study.
33314139	3	31	theme	different	436:444	arg1	AACs					472:475	AACs	472:475	AACs	472:475	RESULTS Two groups of rice with different apparent amylose content (AACs) were used in this study.
33314139	0	32	theme	varieties	86:94	arg1	quality					70:76	the eating quality	59:76	the eating quality of rice varieties with similar amylose contents	59:124	Influence of starch fine structure and storage proteins on the eating quality of rice varieties with similar amylose contents.
33314139	3	33	theme	rice	426:429	arg1	rice					426:429	rice	426:429	rice	426:429	RESULTS Two groups of rice with different apparent amylose content (AACs) were used in this study.
33314139	3	33	theme	rice	426:429	arg1	groups					416:421	Two groups	412:421	Two groups of rice with different apparent amylose content (AACs)	412:476	RESULTS Two groups of rice with different apparent amylose content (AACs) were used in this study.
33314139	7	34	theme	granules	1101:1108	arg1	center					1084:1089	the center	1080:1089	the center of starch granules	1080:1108	In addition, the low-AAC varieties produced opaque endosperms, which may result from an increased number of pores in the center of starch granules.
33314139	10	35	theme	rice	1542:1545	arg1	quality					1531:1537	eating quality	1524:1537	eating quality of rice	1524:1545	By contrast, long branch-chain amylopectin, long-chain amylopectin (DP 35-60), glutelin and prolamin had adverse effects on eating quality of rice.
33314139	0	36	theme	starch	13:18	arg1	structure					25:33	starch fine structure	13:33	starch fine structure	13:33	Influence of starch fine structure and storage proteins on the eating quality of rice varieties with similar amylose contents.
33314139	9	37	theme	branch-chain	1260:1271	arg1	amylopectin					1273:1283	short branch-chain amylopectin	1254:1283	short branch-chain amylopectin	1254:1283	In both groups, short branch-chain amylopectin, short-chain amylopectin [degree of polymerization (DP) 6-12], and albumin had positive effects on eating quality.
33314139	3	38	theme	amylose	455:461	arg1	content					463:469	different apparent amylose content	436:469	different apparent amylose content (AACs)	436:476	RESULTS Two groups of rice with different apparent amylose content (AACs) were used in this study.
33314139	3	38	theme	amylose	455:461	arg1	AACs					472:475	AACs	472:475	AACs	472:475	RESULTS Two groups of rice with different apparent amylose content (AACs) were used in this study.
33314139	0	39	theme	structure	25:33	arg1	Influence					0:8	Influence	0:8	Influence of starch fine structure and storage proteins on the eating quality of rice varieties with similar amylose contents.	0:125	Influence of starch fine structure and storage proteins on the eating quality of rice varieties with similar amylose contents.
33314139	5	40	theme	groups	765:770	arg1	properties					670:679	The physicochemical properties	650:679	The physicochemical properties	650:679	The physicochemical properties, starch fine structure and crystallinity and storage protein composition of the two groups were analyzed.
33314139	5	40	theme	groups	765:770	arg1	crystallinity					708:720	crystallinity	708:720	crystallinity	708:720	The physicochemical properties, starch fine structure and crystallinity and storage protein composition of the two groups were analyzed.
33314139	5	40	theme	groups	765:770	arg1	structure					694:702	starch fine structure	682:702	starch fine structure	682:702	The physicochemical properties, starch fine structure and crystallinity and storage protein composition of the two groups were analyzed.
33314139	5	40	theme	groups	765:770	arg1	composition					742:752	storage protein composition	726:752	storage protein composition	726:752	The physicochemical properties, starch fine structure and crystallinity and storage protein composition of the two groups were analyzed.
33314139	9	41	theme	amylopectin	1298:1308	arg1	[degree					1310:1316	short-chain amylopectin [degree	1286:1316	short-chain amylopectin [degree of polymerization (DP) 6-12]	1286:1345	In both groups, short branch-chain amylopectin, short-chain amylopectin [degree of polymerization (DP) 6-12], and albumin had positive effects on eating quality.
33314139	7	42	from	number	1061:1066	arg1	center					1084:1089	the center	1080:1089	the center of starch granules	1080:1108	In addition, the low-AAC varieties produced opaque endosperms, which may result from an increased number of pores in the center of starch granules.
33314139	10	43	theme	branch-chain	1418:1429	arg1	amylopectin					1431:1441	long branch-chain amylopectin	1413:1441	long branch-chain amylopectin	1413:1441	By contrast, long branch-chain amylopectin, long-chain amylopectin (DP 35-60), glutelin and prolamin had adverse effects on eating quality of rice.
33314139	4	44	with	varieties	609:617	arg1	AACs					631:634	medium AACs	624:634	medium AACs (17.2-17.5%)	624:647	One group contained four varieties with low AACs (8.8-9.4%), whereas the other contained four traditional varieties with medium AACs (17.2-17.5%).
33314139	4	44	with	varieties	609:617	arg1	%					646:646	17.2-17.5%	637:646	17.2-17.5%	637:646	One group contained four varieties with low AACs (8.8-9.4%), whereas the other contained four traditional varieties with medium AACs (17.2-17.5%).
33314139	1	45	theme	rice	219:222	arg1	varieties					224:232	new rice varieties	215:232	new rice varieties with excellent eating quality from farmers	215:275	BACKGROUND Rice eating quality largely dictates consumer preference, and the demand for new rice varieties with excellent eating quality from farmers is increasing.
33314139	6	46	theme	more	866:869	arg1	amylopectin					883:893	more short-chain amylopectin	866:893	more short-chain amylopectin	866:893	We found that, in both groups, the rice varieties with high eating quality had more short-chain amylopectin, lower glutelin and prolamin content, and a higher albumin content.
33314139	7	47	theme	increased	1051:1059	arg1	number					1061:1066	an increased number	1048:1066	an increased number of pores in the center of starch granules	1048:1108	In addition, the low-AAC varieties produced opaque endosperms, which may result from an increased number of pores in the center of starch granules.
33314139	1	48	theme	eating	249:254	arg1	quality					256:262	excellent eating quality	239:262	excellent eating quality	239:262	BACKGROUND Rice eating quality largely dictates consumer preference, and the demand for new rice varieties with excellent eating quality from farmers is increasing.
33314139	2	49	theme	factors	310:316	arg1	Identification					292:305	Identification	292:305	Identification of factors contributing to eating quality	292:347	Identification of factors contributing to eating quality is helpful for developing high-quality rice varieties.
33314139	6	50	theme	high	842:845	arg1	quality					854:860	high eating quality	842:860	high eating quality	842:860	We found that, in both groups, the rice varieties with high eating quality had more short-chain amylopectin, lower glutelin and prolamin content, and a higher albumin content.
33314139	11	51	theme	Chemical	1566:1573	arg1	Industry					1575:1582	Chemical Industry	1566:1582	Chemical Industry	1566:1582	© 2020 Society of Chemical Industry.
33314139	6	52	contain	had	862:864	arg2	content					924:930	lower glutelin and prolamin content	896:930	lower glutelin and prolamin content	896:930	We found that, in both groups, the rice varieties with high eating quality had more short-chain amylopectin, lower glutelin and prolamin content, and a higher albumin content.
33314139	6	52	contain	had	862:864	arg2	content					954:960	a higher albumin content	937:960	a higher albumin content	937:960	We found that, in both groups, the rice varieties with high eating quality had more short-chain amylopectin, lower glutelin and prolamin content, and a higher albumin content.
33314139	6	52	contain	had	862:864	arg1	varieties					827:835	the rice varieties	818:835	the rice varieties with high eating quality	818:860	We found that, in both groups, the rice varieties with high eating quality had more short-chain amylopectin, lower glutelin and prolamin content, and a higher albumin content.
33314139	6	52	contain	had	862:864	arg2	amylopectin					883:893	more short-chain amylopectin	866:893	more short-chain amylopectin	866:893	We found that, in both groups, the rice varieties with high eating quality had more short-chain amylopectin, lower glutelin and prolamin content, and a higher albumin content.
33314139	0	53	theme	storage	39:45	arg1	proteins					47:54	storage proteins	39:54	storage proteins	39:54	Influence of starch fine structure and storage proteins on the eating quality of rice varieties with similar amylose contents.
33314139	4	54	theme	medium	624:629	arg1	AACs					631:634	medium AACs	624:634	medium AACs (17.2-17.5%)	624:647	One group contained four varieties with low AACs (8.8-9.4%), whereas the other contained four traditional varieties with medium AACs (17.2-17.5%).
33314139	4	54	theme	medium	624:629	arg1	%					646:646	17.2-17.5%	637:646	17.2-17.5%	637:646	One group contained four varieties with low AACs (8.8-9.4%), whereas the other contained four traditional varieties with medium AACs (17.2-17.5%).
33314139	10	55	theme	adverse	1505:1511	arg1	effects					1513:1519	adverse effects	1505:1519	adverse effects	1505:1519	By contrast, long branch-chain amylopectin, long-chain amylopectin (DP 35-60), glutelin and prolamin had adverse effects on eating quality of rice.
33314139	4	56	with	varieties	528:536	arg1	AACs					547:550	low AACs	543:550	low AACs (8.8-9.4%)	543:561	One group contained four varieties with low AACs (8.8-9.4%), whereas the other contained four traditional varieties with medium AACs (17.2-17.5%).
33314139	4	56	with	varieties	528:536	arg1	%					560:560	8.8-9.4%	553:560	8.8-9.4%	553:560	One group contained four varieties with low AACs (8.8-9.4%), whereas the other contained four traditional varieties with medium AACs (17.2-17.5%).
33314139	1	57	theme	consumer	175:182	arg1	preference					184:193	consumer preference	175:193	consumer preference	175:193	BACKGROUND Rice eating quality largely dictates consumer preference, and the demand for new rice varieties with excellent eating quality from farmers is increasing.
33314139	8	58	theme	storage	1165:1171	arg1	composition					1181:1191	the storage protein composition	1161:1191	the storage protein composition	1161:1191	CONCLUSIONS Both the fine structure of starch and the storage protein composition were closely related to rice eating quality.
33314139	7	59	theme	opaque	1007:1012	arg1	endosperms					1014:1023	opaque endosperms	1007:1023	opaque endosperms	1007:1023	In addition, the low-AAC varieties produced opaque endosperms, which may result from an increased number of pores in the center of starch granules.
33314139	9	60	theme	eating	1384:1389	arg1	quality					1391:1397	eating quality	1384:1397	eating quality	1384:1397	In both groups, short branch-chain amylopectin, short-chain amylopectin [degree of polymerization (DP) 6-12], and albumin had positive effects on eating quality.
33314139	8	61	theme	fine	1132:1135	arg1	structure					1137:1145	the fine structure	1128:1145	the fine structure of starch	1128:1155	CONCLUSIONS Both the fine structure of starch and the storage protein composition were closely related to rice eating quality.
33314139	6	62	theme	lower	896:900	arg1	content					924:930	lower glutelin and prolamin content	896:930	lower glutelin and prolamin content	896:930	We found that, in both groups, the rice varieties with high eating quality had more short-chain amylopectin, lower glutelin and prolamin content, and a higher albumin content.
33314139	5	63	theme	fine	689:692	arg1	structure					694:702	starch fine structure	682:702	starch fine structure	682:702	The physicochemical properties, starch fine structure and crystallinity and storage protein composition of the two groups were analyzed.
33314139	0	64	theme	amylose	109:115	arg1	contents					117:124	similar amylose contents	101:124	similar amylose contents	101:124	Influence of starch fine structure and storage proteins on the eating quality of rice varieties with similar amylose contents.
33314139	8	65	theme	eating	1222:1227	arg1	quality					1229:1235	rice eating quality	1217:1235	rice eating quality	1217:1235	CONCLUSIONS Both the fine structure of starch and the storage protein composition were closely related to rice eating quality.
33314139	1	66	theme	BACKGROUND	127:136	arg1	quality					150:156	BACKGROUND Rice eating quality	127:156	BACKGROUND Rice eating quality	127:156	BACKGROUND Rice eating quality largely dictates consumer preference, and the demand for new rice varieties with excellent eating quality from farmers is increasing.
33314139	5	67	theme	physicochemical	654:668	arg1	properties					670:679	The physicochemical properties	650:679	The physicochemical properties	650:679	The physicochemical properties, starch fine structure and crystallinity and storage protein composition of the two groups were analyzed.
33314139	5	68	theme	protein	734:740	arg1	composition					742:752	storage protein composition	726:752	storage protein composition	726:752	The physicochemical properties, starch fine structure and crystallinity and storage protein composition of the two groups were analyzed.
33314139	1	69	theme	eating	143:148	arg1	quality					150:156	BACKGROUND Rice eating quality	127:156	BACKGROUND Rice eating quality	127:156	BACKGROUND Rice eating quality largely dictates consumer preference, and the demand for new rice varieties with excellent eating quality from farmers is increasing.
33314139	6	70	theme	higher	939:944	arg1	content					954:960	a higher albumin content	937:960	a higher albumin content	937:960	We found that, in both groups, the rice varieties with high eating quality had more short-chain amylopectin, lower glutelin and prolamin content, and a higher albumin content.
33314139	9	71	contain	had	1360:1362	arg1	[degree					1310:1316	short-chain amylopectin [degree	1286:1316	short-chain amylopectin [degree of polymerization (DP) 6-12]	1286:1345	In both groups, short branch-chain amylopectin, short-chain amylopectin [degree of polymerization (DP) 6-12], and albumin had positive effects on eating quality.
33314139	9	71	contain	had	1360:1362	arg1	amylopectin					1273:1283	short branch-chain amylopectin	1254:1283	short branch-chain amylopectin	1254:1283	In both groups, short branch-chain amylopectin, short-chain amylopectin [degree of polymerization (DP) 6-12], and albumin had positive effects on eating quality.
33314139	9	71	contain	had	1360:1362	arg1	albumin					1352:1358	albumin	1352:1358	albumin	1352:1358	In both groups, short branch-chain amylopectin, short-chain amylopectin [degree of polymerization (DP) 6-12], and albumin had positive effects on eating quality.
33314139	9	71	contain	had	1360:1362	arg2	effects					1373:1379	positive effects	1364:1379	positive effects	1364:1379	In both groups, short branch-chain amylopectin, short-chain amylopectin [degree of polymerization (DP) 6-12], and albumin had positive effects on eating quality.
33314139	0	72	theme	rice	81:84	arg1	varieties					86:94	rice varieties	81:94	rice varieties	81:94	Influence of starch fine structure and storage proteins on the eating quality of rice varieties with similar amylose contents.
33314139	7	73	from	center	1084:1089	arg1	number					1061:1066	an increased number	1048:1066	an increased number of pores in the center of starch granules	1048:1108	In addition, the low-AAC varieties produced opaque endosperms, which may result from an increased number of pores in the center of starch granules.
33314139	1	74	theme	new	215:217	arg1	varieties					224:232	new rice varieties	215:232	new rice varieties with excellent eating quality from farmers	215:275	BACKGROUND Rice eating quality largely dictates consumer preference, and the demand for new rice varieties with excellent eating quality from farmers is increasing.
33314139	3	75	theme	apparent	446:453	arg1	content					463:469	different apparent amylose content	436:469	different apparent amylose content (AACs)	436:476	RESULTS Two groups of rice with different apparent amylose content (AACs) were used in this study.
33314139	3	75	theme	apparent	446:453	arg1	AACs					472:475	AACs	472:475	AACs	472:475	RESULTS Two groups of rice with different apparent amylose content (AACs) were used in this study.
33314139	7	76	theme	starch	1094:1099	arg1	granules					1101:1108	starch granules	1094:1108	starch granules	1094:1108	In addition, the low-AAC varieties produced opaque endosperms, which may result from an increased number of pores in the center of starch granules.
33314139	4	77	theme	low	543:545	arg1	AACs					547:550	low AACs	543:550	low AACs (8.8-9.4%)	543:561	One group contained four varieties with low AACs (8.8-9.4%), whereas the other contained four traditional varieties with medium AACs (17.2-17.5%).
33314139	4	77	theme	low	543:545	arg1	%					560:560	8.8-9.4%	553:560	8.8-9.4%	553:560	One group contained four varieties with low AACs (8.8-9.4%), whereas the other contained four traditional varieties with medium AACs (17.2-17.5%).
33314139	2	78	theme	high-quality	375:386	arg1	varieties					393:401	high-quality rice varieties	375:401	high-quality rice varieties	375:401	Identification of factors contributing to eating quality is helpful for developing high-quality rice varieties.
33314139	0	79	theme	fine	20:23	arg1	structure					25:33	starch fine structure	13:33	starch fine structure	13:33	Influence of starch fine structure and storage proteins on the eating quality of rice varieties with similar amylose contents.
33314139	9	80	theme	short	1254:1258	arg1	amylopectin					1273:1283	short branch-chain amylopectin	1254:1283	short branch-chain amylopectin	1254:1283	In both groups, short branch-chain amylopectin, short-chain amylopectin [degree of polymerization (DP) 6-12], and albumin had positive effects on eating quality.
33314139	0	81	theme	proteins	47:54	arg1	Influence					0:8	Influence	0:8	Influence of starch fine structure and storage proteins on the eating quality of rice varieties with similar amylose contents.	0:125	Influence of starch fine structure and storage proteins on the eating quality of rice varieties with similar amylose contents.
33314139	9	82	theme	short-chain	1286:1296	arg1	[degree					1310:1316	short-chain amylopectin [degree	1286:1316	short-chain amylopectin [degree of polymerization (DP) 6-12]	1286:1345	In both groups, short branch-chain amylopectin, short-chain amylopectin [degree of polymerization (DP) 6-12], and albumin had positive effects on eating quality.
33314139	10	83	contain	had	1501:1503	arg1	amylopectin					1431:1441	long branch-chain amylopectin	1413:1441	long branch-chain amylopectin	1413:1441	By contrast, long branch-chain amylopectin, long-chain amylopectin (DP 35-60), glutelin and prolamin had adverse effects on eating quality of rice.
33314139	10	83	contain	had	1501:1503	arg2	effects					1513:1519	adverse effects	1505:1519	adverse effects	1505:1519	By contrast, long branch-chain amylopectin, long-chain amylopectin (DP 35-60), glutelin and prolamin had adverse effects on eating quality of rice.
33314139	10	83	contain	had	1501:1503	arg1	glutelin					1479:1486	glutelin	1479:1486	glutelin	1479:1486	By contrast, long branch-chain amylopectin, long-chain amylopectin (DP 35-60), glutelin and prolamin had adverse effects on eating quality of rice.
33314139	10	83	contain	had	1501:1503	arg1	amylopectin					1455:1465	long-chain amylopectin	1444:1465	long-chain amylopectin (DP 35-60)	1444:1476	By contrast, long branch-chain amylopectin, long-chain amylopectin (DP 35-60), glutelin and prolamin had adverse effects on eating quality of rice.
33314139	10	83	contain	had	1501:1503	arg1	DP					1468:1469	DP 35-60	1468:1475	DP 35-60	1468:1475	By contrast, long branch-chain amylopectin, long-chain amylopectin (DP 35-60), glutelin and prolamin had adverse effects on eating quality of rice.
33314139	10	83	contain	had	1501:1503	arg1	prolamin					1492:1499	prolamin	1492:1499	prolamin	1492:1499	By contrast, long branch-chain amylopectin, long-chain amylopectin (DP 35-60), glutelin and prolamin had adverse effects on eating quality of rice.
33314139	3	84	used	used	483:486	arg2	rice					426:429	rice	426:429	rice	426:429	RESULTS Two groups of rice with different apparent amylose content (AACs) were used in this study.
33314139	3	84	used	used	483:486	arg2	groups					416:421	Two groups	412:421	Two groups of rice with different apparent amylose content (AACs)	412:476	RESULTS Two groups of rice with different apparent amylose content (AACs) were used in this study.
33314139	2	85	theme	eating	334:339	arg1	quality					341:347	eating quality	334:347	eating quality	334:347	Identification of factors contributing to eating quality is helpful for developing high-quality rice varieties.
33314139	10	86	theme	long	1413:1416	arg1	amylopectin					1431:1441	long branch-chain amylopectin	1413:1441	long branch-chain amylopectin	1413:1441	By contrast, long branch-chain amylopectin, long-chain amylopectin (DP 35-60), glutelin and prolamin had adverse effects on eating quality of rice.
33314139	9	87	theme	polymerization	1321:1334	arg1	amylopectin					1273:1283	short branch-chain amylopectin	1254:1283	short branch-chain amylopectin	1254:1283	In both groups, short branch-chain amylopectin, short-chain amylopectin [degree of polymerization (DP) 6-12], and albumin had positive effects on eating quality.
33314139	9	87	theme	polymerization	1321:1334	arg1	albumin					1352:1358	albumin	1352:1358	albumin	1352:1358	In both groups, short branch-chain amylopectin, short-chain amylopectin [degree of polymerization (DP) 6-12], and albumin had positive effects on eating quality.
33314139	9	87	theme	polymerization	1321:1334	arg1	[degree					1310:1316	short-chain amylopectin [degree	1286:1316	short-chain amylopectin [degree of polymerization (DP) 6-12]	1286:1345	In both groups, short branch-chain amylopectin, short-chain amylopectin [degree of polymerization (DP) 6-12], and albumin had positive effects on eating quality.
33314139	1	88	with	varieties	224:232	arg1	quality					256:262	excellent eating quality	239:262	excellent eating quality	239:262	BACKGROUND Rice eating quality largely dictates consumer preference, and the demand for new rice varieties with excellent eating quality from farmers is increasing.
33314139	6	89	with	varieties	827:835	arg1	quality					854:860	high eating quality	842:860	high eating quality	842:860	We found that, in both groups, the rice varieties with high eating quality had more short-chain amylopectin, lower glutelin and prolamin content, and a higher albumin content.
32553968	9	0	from	medium	1336:1341	arg1	cells					1313:1317	separate algal cells	1298:1317	separate algal cells from the culture medium	1298:1341	This first report of using commercial cationic cassava starch as a flocculant provides a low cost and convenient process to separate algal cells from the culture medium.
32553968	1	1	with	composites	165:174	arg1	particles					190:198	magnetic particles	181:198	magnetic particles	181:198	Commercial- and laboratory modified- cationic cassava starches and their composites with magnetic particles were examined for characteristics and separation efficiency.
32553968	7	2	theme	commercial	1006:1015	arg1	starches					1026:1033	the commercial cationic starches	1002:1033	the commercial cationic starches	1002:1033	Their composites showed lower separation efficiency than the commercial cationic starches.
32553968	0	3	theme	biomass	72:78	arg1	separation					80:89	microalgal biomass separation	61:89	microalgal biomass separation	61:89	Cationic cassava starch and its composite as flocculants for microalgal biomass separation.
32553968	9	4	theme	commercial	1201:1210	arg1	starch					1229:1234	commercial cationic cassava starch	1201:1234	commercial cationic cassava starch	1201:1234	This first report of using commercial cationic cassava starch as a flocculant provides a low cost and convenient process to separate algal cells from the culture medium.
32553968	9	4	theme	commercial	1201:1210	arg1	flocculant					1241:1250	a flocculant	1239:1250	a flocculant	1239:1250	This first report of using commercial cationic cassava starch as a flocculant provides a low cost and convenient process to separate algal cells from the culture medium.
32553968	9	5	theme	low	1263:1265	arg1	process					1287:1293	a low cost and convenient process	1261:1293	a low cost and convenient process to separate algal cells from the culture medium	1261:1341	This first report of using commercial cationic cassava starch as a flocculant provides a low cost and convenient process to separate algal cells from the culture medium.
32553968	6	6	theme	lower	918:922	arg1	dosage					924:929	a lower dosage	916:929	a lower dosage of 0.1 g L-1	916:942	The maximum recovery capacity (10.20 ± 0.16 g DCW g starch-1) was recorded by using commercial cationic starch with DS 0.040 at a lower dosage of 0.1 g L-1.
32553968	8	7	theme	cationic	1076:1083	arg1	starch					1093:1098	the commercial cationic cassava starch	1061:1098	the commercial cationic cassava starch with 0.040 DS	1061:1112	The results suggest that the commercial cationic cassava starch with 0.040 DS shows great potential as a flocculant for algal separation.
32553968	7	8	theme	lower	969:973	arg1	efficiency					986:995	lower separation efficiency	969:995	lower separation efficiency than the commercial cationic starches	969:1033	Their composites showed lower separation efficiency than the commercial cationic starches.
32553968	2	9	theme	increasing	327:336	arg1	degree					338:343	an increasing degree	324:343	an increasing degree of substitution (DS) value (0.0180 to 0.91)	324:387	Scanning electron micrographs showed that cationic starch with an increasing degree of substitution (DS) value (0.0180 to 0.91) showed greater clumped polyhedral granules and became markedly enlarged with disintegrated boundaries.
32553968	4	10	theme	commercial	693:702	arg1	starch					713:718	commercial cationic starch	693:718	commercial cationic starch with DS 0.040 at 1.0 g L-1	693:745	The maximum harvesting efficiency of 92.86 ± 0.46% was achieved when commercial cationic starch with DS 0.040 at 1.0 g L-1 was added to the Chlorella sp.
32553968	9	11	theme	first	1179:1183	arg1	report					1185:1190	This first report	1174:1190	This first report of using commercial cationic cassava starch as a flocculant	1174:1250	This first report of using commercial cationic cassava starch as a flocculant provides a low cost and convenient process to separate algal cells from the culture medium.
32553968	9	12	theme	culture	1328:1334	arg1	medium					1336:1341	the culture medium	1324:1341	the culture medium	1324:1341	This first report of using commercial cationic cassava starch as a flocculant provides a low cost and convenient process to separate algal cells from the culture medium.
32553968	8	13	theme	commercial	1065:1074	arg1	starch					1093:1098	the commercial cationic cassava starch	1061:1098	the commercial cationic cassava starch with 0.040 DS	1061:1112	The results suggest that the commercial cationic cassava starch with 0.040 DS shows great potential as a flocculant for algal separation.
32553968	6	14	theme	g	838:838	arg1	starch-1					840:847	10.20 ± 0.16 g DCW g starch-1	819:847	10.20 ± 0.16 g DCW g starch-1	819:847	The maximum recovery capacity (10.20 ± 0.16 g DCW g starch-1) was recorded by using commercial cationic starch with DS 0.040 at a lower dosage of 0.1 g L-1.
32553968	6	14	theme	g	838:838	arg1	capacity					809:816	The maximum recovery capacity	788:816	The maximum recovery capacity (10.20 ± 0.16 g DCW g starch-1)	788:848	The maximum recovery capacity (10.20 ± 0.16 g DCW g starch-1) was recorded by using commercial cationic starch with DS 0.040 at a lower dosage of 0.1 g L-1.
32553968	8	15	with	starch	1093:1098	arg1	DS					1111:1112	0.040 DS	1105:1112	0.040 DS	1105:1112	The results suggest that the commercial cationic cassava starch with 0.040 DS shows great potential as a flocculant for algal separation.
32553968	10	16	from	range	1411:1415	arg1	biotechnologies					1465:1479	food and pharmaceutical biotechnologies	1441:1479	food and pharmaceutical biotechnologies	1441:1479	Moreover, uncontaminated magnetic particle biomass allows for wide range of algal utilization in food and pharmaceutical biotechnologies.
32553968	10	17	from	utilization	1426:1436	arg1	biotechnologies					1465:1479	food and pharmaceutical biotechnologies	1441:1479	food and pharmaceutical biotechnologies	1441:1479	Moreover, uncontaminated magnetic particle biomass allows for wide range of algal utilization in food and pharmaceutical biotechnologies.
32553968	3	18	from	value	553:557	arg1	starches					571:578	cationic starches	562:578	cationic starches	562:578	Zeta potential analysis revealed that the increase in the DS value in cationic starches resulted in an increase in positive charge.
32553968	1	19	theme	magnetic	181:188	arg1	particles					190:198	magnetic particles	181:198	magnetic particles	181:198	Commercial- and laboratory modified- cationic cassava starches and their composites with magnetic particles were examined for characteristics and separation efficiency.
32553968	1	20	with	starches	146:153	arg1	particles					190:198	magnetic particles	181:198	magnetic particles	181:198	Commercial- and laboratory modified- cationic cassava starches and their composites with magnetic particles were examined for characteristics and separation efficiency.
32553968	6	21	theme	10.20 ± 0.16 g	819:832	arg1	starch-1					840:847	10.20 ± 0.16 g DCW g starch-1	819:847	10.20 ± 0.16 g DCW g starch-1	819:847	The maximum recovery capacity (10.20 ± 0.16 g DCW g starch-1) was recorded by using commercial cationic starch with DS 0.040 at a lower dosage of 0.1 g L-1.
32553968	6	21	theme	10.20 ± 0.16 g	819:832	arg1	capacity					809:816	The maximum recovery capacity	788:816	The maximum recovery capacity (10.20 ± 0.16 g DCW g starch-1)	788:848	The maximum recovery capacity (10.20 ± 0.16 g DCW g starch-1) was recorded by using commercial cationic starch with DS 0.040 at a lower dosage of 0.1 g L-1.
32553968	0	22	theme	Cationic	0:7	arg1	starch					17:22	Cationic cassava starch	0:22	Cationic cassava starch	0:22	Cationic cassava starch and its composite as flocculants for microalgal biomass separation.
32553968	9	23	theme	cost	1267:1270	arg1	process					1287:1293	a low cost and convenient process	1261:1293	a low cost and convenient process to separate algal cells from the culture medium	1261:1341	This first report of using commercial cationic cassava starch as a flocculant provides a low cost and convenient process to separate algal cells from the culture medium.
32553968	2	24	theme	clumped	404:410	arg1	granules					423:430	greater clumped polyhedral granules	396:430	greater clumped polyhedral granules	396:430	Scanning electron micrographs showed that cationic starch with an increasing degree of substitution (DS) value (0.0180 to 0.91) showed greater clumped polyhedral granules and became markedly enlarged with disintegrated boundaries.
32553968	0	25	theme	cassava	9:15	arg1	starch					17:22	Cationic cassava starch	0:22	Cationic cassava starch	0:22	Cationic cassava starch and its composite as flocculants for microalgal biomass separation.
32553968	4	26	theme	Chlorella	764:772	arg1	sp					774:775	the Chlorella sp	760:775	the Chlorella sp	760:775	The maximum harvesting efficiency of 92.86 ± 0.46% was achieved when commercial cationic starch with DS 0.040 at 1.0 g L-1 was added to the Chlorella sp.
32553968	2	27	theme	disintegrated	466:478	arg1	boundaries					480:489	disintegrated boundaries	466:489	disintegrated boundaries	466:489	Scanning electron micrographs showed that cationic starch with an increasing degree of substitution (DS) value (0.0180 to 0.91) showed greater clumped polyhedral granules and became markedly enlarged with disintegrated boundaries.
32553968	9	28	theme	separate	1298:1305	arg1	cells					1313:1317	separate algal cells	1298:1317	separate algal cells from the culture medium	1298:1341	This first report of using commercial cationic cassava starch as a flocculant provides a low cost and convenient process to separate algal cells from the culture medium.
32553968	10	29	theme	food	1441:1444	arg1	biotechnologies					1465:1479	food and pharmaceutical biotechnologies	1441:1479	food and pharmaceutical biotechnologies	1441:1479	Moreover, uncontaminated magnetic particle biomass allows for wide range of algal utilization in food and pharmaceutical biotechnologies.
32553968	1	30	theme	Commercial-	92:102	arg1	starches					146:153	Commercial- and laboratory modified- cationic cassava starches	92:153	Commercial- and laboratory modified- cationic cassava starches	92:153	Commercial- and laboratory modified- cationic cassava starches and their composites with magnetic particles were examined for characteristics and separation efficiency.
32553968	4	31	theme	cationic	704:711	arg1	starch					713:718	commercial cationic starch	693:718	commercial cationic starch with DS 0.040 at 1.0 g L-1	693:745	The maximum harvesting efficiency of 92.86 ± 0.46% was achieved when commercial cationic starch with DS 0.040 at 1.0 g L-1 was added to the Chlorella sp.
32553968	10	32	from	biotechnologies	1465:1479	arg1	range					1411:1415	wide range	1406:1415	wide range of algal utilization in food and pharmaceutical biotechnologies	1406:1479	Moreover, uncontaminated magnetic particle biomass allows for wide range of algal utilization in food and pharmaceutical biotechnologies.
32553968	3	33	from	increase	534:541	arg1	value					553:557	the DS value	546:557	the DS value in cationic starches	546:578	Zeta potential analysis revealed that the increase in the DS value in cationic starches resulted in an increase in positive charge.
32553968	10	34	theme	utilization	1426:1436	arg1	range					1411:1415	wide range	1406:1415	wide range of algal utilization in food and pharmaceutical biotechnologies	1406:1479	Moreover, uncontaminated magnetic particle biomass allows for wide range of algal utilization in food and pharmaceutical biotechnologies.
32553968	3	35	theme	potential	497:505	arg1	analysis					507:514	Zeta potential analysis	492:514	Zeta potential analysis	492:514	Zeta potential analysis revealed that the increase in the DS value in cationic starches resulted in an increase in positive charge.
32553968	2	36	theme	substitution	348:359	arg1	value					366:370	substitution (DS) value	348:370	substitution (DS) value (0.0180 to 0.91)	348:387	Scanning electron micrographs showed that cationic starch with an increasing degree of substitution (DS) value (0.0180 to 0.91) showed greater clumped polyhedral granules and became markedly enlarged with disintegrated boundaries.
32553968	2	36	theme	substitution	348:359	arg1	0.91					383:386	0.91	383:386	0.91	383:386	Scanning electron micrographs showed that cationic starch with an increasing degree of substitution (DS) value (0.0180 to 0.91) showed greater clumped polyhedral granules and became markedly enlarged with disintegrated boundaries.
32553968	10	37	theme	uncontaminated	1354:1367	arg1	biomass					1387:1393	uncontaminated magnetic particle biomass	1354:1393	uncontaminated magnetic particle biomass	1354:1393	Moreover, uncontaminated magnetic particle biomass allows for wide range of algal utilization in food and pharmaceutical biotechnologies.
32553968	4	38	with	starch	713:718	arg1	DS					725:726	DS 0.040	725:732	DS 0.040	725:732	The maximum harvesting efficiency of 92.86 ± 0.46% was achieved when commercial cationic starch with DS 0.040 at 1.0 g L-1 was added to the Chlorella sp.
32553968	1	39	theme	laboratory	108:117	arg1	starches					146:153	Commercial- and laboratory modified- cationic cassava starches	92:153	Commercial- and laboratory modified- cationic cassava starches	92:153	Commercial- and laboratory modified- cationic cassava starches and their composites with magnetic particles were examined for characteristics and separation efficiency.
32553968	7	40	theme	separation	975:984	arg1	efficiency					986:995	lower separation efficiency	969:995	lower separation efficiency than the commercial cationic starches	969:1033	Their composites showed lower separation efficiency than the commercial cationic starches.
32553968	2	41	theme	value	366:370	arg1	degree					338:343	an increasing degree	324:343	an increasing degree of substitution (DS) value (0.0180 to 0.91)	324:387	Scanning electron micrographs showed that cationic starch with an increasing degree of substitution (DS) value (0.0180 to 0.91) showed greater clumped polyhedral granules and became markedly enlarged with disintegrated boundaries.
32553968	3	42	theme	cationic	562:569	arg1	starches					571:578	cationic starches	562:578	cationic starches	562:578	Zeta potential analysis revealed that the increase in the DS value in cationic starches resulted in an increase in positive charge.
32553968	2	43	theme	electron	270:277	arg1	micrographs					279:289	Scanning electron micrographs	261:289	Scanning electron micrographs	261:289	Scanning electron micrographs showed that cationic starch with an increasing degree of substitution (DS) value (0.0180 to 0.91) showed greater clumped polyhedral granules and became markedly enlarged with disintegrated boundaries.
32553968	6	44	with	starch	892:897	arg1	DS					904:905	DS 0.040	904:911	DS 0.040	904:911	The maximum recovery capacity (10.20 ± 0.16 g DCW g starch-1) was recorded by using commercial cationic starch with DS 0.040 at a lower dosage of 0.1 g L-1.
32553968	2	45	theme	polyhedral	412:421	arg1	granules					423:430	greater clumped polyhedral granules	396:430	greater clumped polyhedral granules	396:430	Scanning electron micrographs showed that cationic starch with an increasing degree of substitution (DS) value (0.0180 to 0.91) showed greater clumped polyhedral granules and became markedly enlarged with disintegrated boundaries.
32553968	8	46	theme	great	1120:1124	arg1	potential					1126:1134	great potential	1120:1134	great potential	1120:1134	The results suggest that the commercial cationic cassava starch with 0.040 DS shows great potential as a flocculant for algal separation.
32553968	8	46	theme	great	1120:1124	arg1	flocculant					1141:1150	a flocculant	1139:1150	a flocculant for algal separation	1139:1171	The results suggest that the commercial cationic cassava starch with 0.040 DS shows great potential as a flocculant for algal separation.
32553968	6	47	theme	cationic	883:890	arg1	starch					892:897	commercial cationic starch	872:897	commercial cationic starch with DS 0.040	872:911	The maximum recovery capacity (10.20 ± 0.16 g DCW g starch-1) was recorded by using commercial cationic starch with DS 0.040 at a lower dosage of 0.1 g L-1.
32553968	2	48	theme	Scanning	261:268	arg1	micrographs					279:289	Scanning electron micrographs	261:289	Scanning electron micrographs	261:289	Scanning electron micrographs showed that cationic starch with an increasing degree of substitution (DS) value (0.0180 to 0.91) showed greater clumped polyhedral granules and became markedly enlarged with disintegrated boundaries.
32553968	6	49	theme	maximum	792:798	arg1	starch-1					840:847	10.20 ± 0.16 g DCW g starch-1	819:847	10.20 ± 0.16 g DCW g starch-1	819:847	The maximum recovery capacity (10.20 ± 0.16 g DCW g starch-1) was recorded by using commercial cationic starch with DS 0.040 at a lower dosage of 0.1 g L-1.
32553968	6	49	theme	maximum	792:798	arg1	capacity					809:816	The maximum recovery capacity	788:816	The maximum recovery capacity (10.20 ± 0.16 g DCW g starch-1)	788:848	The maximum recovery capacity (10.20 ± 0.16 g DCW g starch-1) was recorded by using commercial cationic starch with DS 0.040 at a lower dosage of 0.1 g L-1.
32553968	1	50	theme	separation	238:247	arg1	efficiency					249:258	separation efficiency	238:258	separation efficiency	238:258	Commercial- and laboratory modified- cationic cassava starches and their composites with magnetic particles were examined for characteristics and separation efficiency.
32553968	9	51	theme	cassava	1221:1227	arg1	starch					1229:1234	commercial cationic cassava starch	1201:1234	commercial cationic cassava starch	1201:1234	This first report of using commercial cationic cassava starch as a flocculant provides a low cost and convenient process to separate algal cells from the culture medium.
32553968	9	51	theme	cassava	1221:1227	arg1	flocculant					1241:1250	a flocculant	1239:1250	a flocculant	1239:1250	This first report of using commercial cationic cassava starch as a flocculant provides a low cost and convenient process to separate algal cells from the culture medium.
32553968	6	52	theme	commercial	872:881	arg1	starch					892:897	commercial cationic starch	872:897	commercial cationic starch with DS 0.040	872:911	The maximum recovery capacity (10.20 ± 0.16 g DCW g starch-1) was recorded by using commercial cationic starch with DS 0.040 at a lower dosage of 0.1 g L-1.
32553968	3	53	theme	positive	607:614	arg1	charge					616:621	positive charge	607:621	positive charge	607:621	Zeta potential analysis revealed that the increase in the DS value in cationic starches resulted in an increase in positive charge.
32553968	10	54	theme	wide	1406:1409	arg1	range					1411:1415	wide range	1406:1415	wide range of algal utilization in food and pharmaceutical biotechnologies	1406:1479	Moreover, uncontaminated magnetic particle biomass allows for wide range of algal utilization in food and pharmaceutical biotechnologies.
32553968	1	55	theme	modified-	119:127	arg1	starches					146:153	Commercial- and laboratory modified- cationic cassava starches	92:153	Commercial- and laboratory modified- cationic cassava starches	92:153	Commercial- and laboratory modified- cationic cassava starches and their composites with magnetic particles were examined for characteristics and separation efficiency.
32553968	4	56	theme	maximum	628:634	arg1	efficiency					647:656	The maximum harvesting efficiency	624:656	The maximum harvesting efficiency of 92.86 ± 0.46%	624:673	The maximum harvesting efficiency of 92.86 ± 0.46% was achieved when commercial cationic starch with DS 0.040 at 1.0 g L-1 was added to the Chlorella sp.
32553968	10	57	theme	algal	1420:1424	arg1	utilization					1426:1436	algal utilization	1420:1436	algal utilization in food and pharmaceutical biotechnologies	1420:1479	Moreover, uncontaminated magnetic particle biomass allows for wide range of algal utilization in food and pharmaceutical biotechnologies.
32553968	9	58	theme	algal	1307:1311	arg1	cells					1313:1317	separate algal cells	1298:1317	separate algal cells from the culture medium	1298:1341	This first report of using commercial cationic cassava starch as a flocculant provides a low cost and convenient process to separate algal cells from the culture medium.
32553968	9	59	theme	convenient	1276:1285	arg1	process					1287:1293	a low cost and convenient process	1261:1293	a low cost and convenient process to separate algal cells from the culture medium	1261:1341	This first report of using commercial cationic cassava starch as a flocculant provides a low cost and convenient process to separate algal cells from the culture medium.
32553968	8	60	theme	cassava	1085:1091	arg1	starch					1093:1098	the commercial cationic cassava starch	1061:1098	the commercial cationic cassava starch with 0.040 DS	1061:1112	The results suggest that the commercial cationic cassava starch with 0.040 DS shows great potential as a flocculant for algal separation.
32553968	6	61	theme	DCW	834:836	arg1	starch-1					840:847	10.20 ± 0.16 g DCW g starch-1	819:847	10.20 ± 0.16 g DCW g starch-1	819:847	The maximum recovery capacity (10.20 ± 0.16 g DCW g starch-1) was recorded by using commercial cationic starch with DS 0.040 at a lower dosage of 0.1 g L-1.
32553968	6	61	theme	DCW	834:836	arg1	capacity					809:816	The maximum recovery capacity	788:816	The maximum recovery capacity (10.20 ± 0.16 g DCW g starch-1)	788:848	The maximum recovery capacity (10.20 ± 0.16 g DCW g starch-1) was recorded by using commercial cationic starch with DS 0.040 at a lower dosage of 0.1 g L-1.
32553968	1	62	theme	cationic	129:136	arg1	starches					146:153	Commercial- and laboratory modified- cationic cassava starches	92:153	Commercial- and laboratory modified- cationic cassava starches	92:153	Commercial- and laboratory modified- cationic cassava starches and their composites with magnetic particles were examined for characteristics and separation efficiency.
32553968	6	63	theme	recovery	800:807	arg1	starch-1					840:847	10.20 ± 0.16 g DCW g starch-1	819:847	10.20 ± 0.16 g DCW g starch-1	819:847	The maximum recovery capacity (10.20 ± 0.16 g DCW g starch-1) was recorded by using commercial cationic starch with DS 0.040 at a lower dosage of 0.1 g L-1.
32553968	6	63	theme	recovery	800:807	arg1	capacity					809:816	The maximum recovery capacity	788:816	The maximum recovery capacity (10.20 ± 0.16 g DCW g starch-1)	788:848	The maximum recovery capacity (10.20 ± 0.16 g DCW g starch-1) was recorded by using commercial cationic starch with DS 0.040 at a lower dosage of 0.1 g L-1.
32553968	10	64	theme	magnetic	1369:1376	arg1	biomass					1387:1393	uncontaminated magnetic particle biomass	1354:1393	uncontaminated magnetic particle biomass	1354:1393	Moreover, uncontaminated magnetic particle biomass allows for wide range of algal utilization in food and pharmaceutical biotechnologies.
32553968	10	65	theme	pharmaceutical	1450:1463	arg1	biotechnologies					1465:1479	food and pharmaceutical biotechnologies	1441:1479	food and pharmaceutical biotechnologies	1441:1479	Moreover, uncontaminated magnetic particle biomass allows for wide range of algal utilization in food and pharmaceutical biotechnologies.
32553968	2	66	theme	cationic	303:310	arg1	starch					312:317	cationic starch	303:317	cationic starch with an increasing degree of substitution (DS) value (0.0180 to 0.91)	303:387	Scanning electron micrographs showed that cationic starch with an increasing degree of substitution (DS) value (0.0180 to 0.91) showed greater clumped polyhedral granules and became markedly enlarged with disintegrated boundaries.
32553968	9	67	theme	cationic	1212:1219	arg1	starch					1229:1234	commercial cationic cassava starch	1201:1234	commercial cationic cassava starch	1201:1234	This first report of using commercial cationic cassava starch as a flocculant provides a low cost and convenient process to separate algal cells from the culture medium.
32553968	9	67	theme	cationic	1212:1219	arg1	flocculant					1241:1250	a flocculant	1239:1250	a flocculant	1239:1250	This first report of using commercial cationic cassava starch as a flocculant provides a low cost and convenient process to separate algal cells from the culture medium.
32553968	1	68	theme	cassava	138:144	arg1	starches					146:153	Commercial- and laboratory modified- cationic cassava starches	92:153	Commercial- and laboratory modified- cationic cassava starches	92:153	Commercial- and laboratory modified- cationic cassava starches and their composites with magnetic particles were examined for characteristics and separation efficiency.
32553968	4	69	theme	harvesting	636:645	arg1	efficiency					647:656	The maximum harvesting efficiency	624:656	The maximum harvesting efficiency of 92.86 ± 0.46%	624:673	The maximum harvesting efficiency of 92.86 ± 0.46% was achieved when commercial cationic starch with DS 0.040 at 1.0 g L-1 was added to the Chlorella sp.
32553968	2	70	dep	0.91	383:386	arg1	to					380:381	to	380:381	to	380:381	Scanning electron micrographs showed that cationic starch with an increasing degree of substitution (DS) value (0.0180 to 0.91) showed greater clumped polyhedral granules and became markedly enlarged with disintegrated boundaries.
32553968	10	71	theme	particle	1378:1385	arg1	biomass					1387:1393	uncontaminated magnetic particle biomass	1354:1393	uncontaminated magnetic particle biomass	1354:1393	Moreover, uncontaminated magnetic particle biomass allows for wide range of algal utilization in food and pharmaceutical biotechnologies.
32553968	7	72	theme	cationic	1017:1024	arg1	starches					1026:1033	the commercial cationic starches	1002:1033	the commercial cationic starches	1002:1033	Their composites showed lower separation efficiency than the commercial cationic starches.
32553968	3	73	from	increase	595:602	arg1	charge					616:621	positive charge	607:621	positive charge	607:621	Zeta potential analysis revealed that the increase in the DS value in cationic starches resulted in an increase in positive charge.
32553968	2	74	with	starch	312:317	arg1	degree					338:343	an increasing degree	324:343	an increasing degree of substitution (DS) value (0.0180 to 0.91)	324:387	Scanning electron micrographs showed that cationic starch with an increasing degree of substitution (DS) value (0.0180 to 0.91) showed greater clumped polyhedral granules and became markedly enlarged with disintegrated boundaries.
32553968	0	75	theme	microalgal	61:70	arg1	separation					80:89	microalgal biomass separation	61:89	microalgal biomass separation	61:89	Cationic cassava starch and its composite as flocculants for microalgal biomass separation.
32553968	3	76	theme	Zeta	492:495	arg1	analysis					507:514	Zeta potential analysis	492:514	Zeta potential analysis	492:514	Zeta potential analysis revealed that the increase in the DS value in cationic starches resulted in an increase in positive charge.
32553968	4	77	theme	%	673:673	arg1	efficiency					647:656	The maximum harvesting efficiency	624:656	The maximum harvesting efficiency of 92.86 ± 0.46%	624:673	The maximum harvesting efficiency of 92.86 ± 0.46% was achieved when commercial cationic starch with DS 0.040 at 1.0 g L-1 was added to the Chlorella sp.
32553968	3	78	theme	DS	550:551	arg1	value					553:557	the DS value	546:557	the DS value in cationic starches	546:578	Zeta potential analysis revealed that the increase in the DS value in cationic starches resulted in an increase in positive charge.
32553968	4	79	from	1.0 g L-1	737:745	arg1	starch					713:718	commercial cationic starch	693:718	commercial cationic starch with DS 0.040 at 1.0 g L-1	693:745	The maximum harvesting efficiency of 92.86 ± 0.46% was achieved when commercial cationic starch with DS 0.040 at 1.0 g L-1 was added to the Chlorella sp.
32553968	8	80	theme	algal	1156:1160	arg1	separation					1162:1171	algal separation	1156:1171	algal separation	1156:1171	The results suggest that the commercial cationic cassava starch with 0.040 DS shows great potential as a flocculant for algal separation.
32553968	6	81	theme	0.1 g L-1	934:942	arg1	dosage					924:929	a lower dosage	916:929	a lower dosage of 0.1 g L-1	916:942	The maximum recovery capacity (10.20 ± 0.16 g DCW g starch-1) was recorded by using commercial cationic starch with DS 0.040 at a lower dosage of 0.1 g L-1.
32553968	2	82	dep	clumped	404:410	arg1	greater					396:402	greater	396:402	greater	396:402	Scanning electron micrographs showed that cationic starch with an increasing degree of substitution (DS) value (0.0180 to 0.91) showed greater clumped polyhedral granules and became markedly enlarged with disintegrated boundaries.
34364549	2	0	theme	%	458:458	arg1	loading					427:433	the loading	423:433	the loading of 0, 1, 3, 5, and 7 wt%	423:458	The prepared CNC-PEGs were further used to reinforce the polyvinyl alcohol (PVA) at the loading of 0, 1, 3, 5, and 7 wt% in order to demonstrate the effects of the PEG length on the properties of the PVA/CNC nanocomposites.
34364549	2	1	theme	nanocomposites	547:560	arg1	properties					521:530	the properties	517:530	the properties of the PVA/CNC nanocomposites	517:560	The prepared CNC-PEGs were further used to reinforce the polyvinyl alcohol (PVA) at the loading of 0, 1, 3, 5, and 7 wt% in order to demonstrate the effects of the PEG length on the properties of the PVA/CNC nanocomposites.
34364549	5	2	theme	better	754:759	arg1	enhancement					761:771	better enhancement	754:771	better enhancement in strength and stiffness	754:797	The shorter graft yields better enhancement in strength and stiffness, attributable to the high efficiency in the stress transfer and high interface adhesion.
34364549	3	3	theme	PEG	563:565	arg1	modification					575:586	PEG surface modification	563:586	PEG surface modification	563:586	PEG surface modification resulted in simultaneous improvements in stiffness and toughness.
34364549	1	4	theme	various	215:221	arg1	lengths					223:229	various lengths	215:229	various lengths (1 k, 2 k, 5 k, and 10 kDa)	215:257	In this study, cellulose nanocrystals (CNCs) with PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa) were prepared via a polydopamine (PDA) mediated method in the aqueous solution.
34364549	1	4	theme	various	215:221	arg1	5 k					242:244	5 k	242:244	5 k	242:244	In this study, cellulose nanocrystals (CNCs) with PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa) were prepared via a polydopamine (PDA) mediated method in the aqueous solution.
34364549	1	4	theme	various	215:221	arg1	10 kDa					251:256	10 kDa	251:256	10 kDa	251:256	In this study, cellulose nanocrystals (CNCs) with PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa) were prepared via a polydopamine (PDA) mediated method in the aqueous solution.
34364549	1	4	theme	various	215:221	arg1	2 k					237:239	2 k	237:239	2 k	237:239	In this study, cellulose nanocrystals (CNCs) with PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa) were prepared via a polydopamine (PDA) mediated method in the aqueous solution.
34364549	1	4	theme	various	215:221	arg1	1 k					232:234	1 k	232:234	1 k	232:234	In this study, cellulose nanocrystals (CNCs) with PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa) were prepared via a polydopamine (PDA) mediated method in the aqueous solution.
34364549	2	5	theme	polyvinyl	396:404	arg1	PVA					415:417	PVA	415:417	PVA	415:417	The prepared CNC-PEGs were further used to reinforce the polyvinyl alcohol (PVA) at the loading of 0, 1, 3, 5, and 7 wt% in order to demonstrate the effects of the PEG length on the properties of the PVA/CNC nanocomposites.
34364549	2	5	theme	polyvinyl	396:404	arg1	alcohol					406:412	the polyvinyl alcohol	392:412	the polyvinyl alcohol (PVA)	392:418	The prepared CNC-PEGs were further used to reinforce the polyvinyl alcohol (PVA) at the loading of 0, 1, 3, 5, and 7 wt% in order to demonstrate the effects of the PEG length on the properties of the PVA/CNC nanocomposites.
34364549	0	6	from	PEG	9:11	arg1	nanocrystals					26:37	cellulose nanocrystals	16:37	cellulose nanocrystals	16:37	Grafting PEG on cellulose nanocrystals via polydopamine chemistry and the effects of PEG graft length on the mechanical performance of composite film.
34364549	4	7	theme	composites	717:726	arg1	properties					703:712	the properties	699:712	the properties of composites	699:726	The graft lengths have noticeable impacts on the properties of composites.
34364549	1	8	theme	lengths	223:229	arg1	grafts					205:210	PEG grafts	201:210	PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa)	201:257	In this study, cellulose nanocrystals (CNCs) with PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa) were prepared via a polydopamine (PDA) mediated method in the aqueous solution.
34364549	0	9	theme	graft	89:93	arg1	length					95:100	PEG graft length	85:100	PEG graft length	85:100	Grafting PEG on cellulose nanocrystals via polydopamine chemistry and the effects of PEG graft length on the mechanical performance of composite film.
34364549	5	10	from	enhancement	761:771	arg1	strength					776:783	strength	776:783	strength	776:783	The shorter graft yields better enhancement in strength and stiffness, attributable to the high efficiency in the stress transfer and high interface adhesion.
34364549	5	10	from	enhancement	761:771	arg1	stiffness					789:797	stiffness	789:797	stiffness	789:797	The shorter graft yields better enhancement in strength and stiffness, attributable to the high efficiency in the stress transfer and high interface adhesion.
34364549	5	11	theme	high	820:823	arg1	efficiency					825:834	the high efficiency	816:834	the high efficiency in the stress transfer and high interface adhesion	816:885	The shorter graft yields better enhancement in strength and stiffness, attributable to the high efficiency in the stress transfer and high interface adhesion.
34364549	5	12	from	efficiency	825:834	arg1	adhesion					878:885	high interface adhesion	863:885	high interface adhesion	863:885	The shorter graft yields better enhancement in strength and stiffness, attributable to the high efficiency in the stress transfer and high interface adhesion.
34364549	5	12	from	efficiency	825:834	arg1	transfer					850:857	the stress transfer	839:857	the stress transfer	839:857	The shorter graft yields better enhancement in strength and stiffness, attributable to the high efficiency in the stress transfer and high interface adhesion.
34364549	0	13	theme	PEG	85:87	arg1	length					95:100	PEG graft length	85:100	PEG graft length	85:100	Grafting PEG on cellulose nanocrystals via polydopamine chemistry and the effects of PEG graft length on the mechanical performance of composite film.
34364549	0	14	from	chemistry	56:64	arg1	performance					120:130	the mechanical performance	105:130	the mechanical performance of composite film	105:148	Grafting PEG on cellulose nanocrystals via polydopamine chemistry and the effects of PEG graft length on the mechanical performance of composite film.
34364549	4	15	contain	have	672:675	arg2	impacts					688:694	noticeable impacts	677:694	noticeable impacts	677:694	The graft lengths have noticeable impacts on the properties of composites.
34364549	4	15	contain	have	672:675	arg1	lengths					664:670	The graft lengths	654:670	The graft lengths	654:670	The graft lengths have noticeable impacts on the properties of composites.
34364549	3	16	theme	surface	567:573	arg1	modification					575:586	PEG surface modification	563:586	PEG surface modification	563:586	PEG surface modification resulted in simultaneous improvements in stiffness and toughness.
34364549	6	17	theme	composites	1034:1043	arg1	toughness					1013:1021	the toughness	1009:1021	the toughness of PVA/CNC composites	1009:1043	The longer PEG graft length yields high graft-matrix entanglement, forming a thicker rubbery coating layer that enhances the toughness of PVA/CNC composites.
34364549	0	18	theme	length	95:100	arg1	effects					74:80	the effects	70:80	the effects of PEG graft length on the mechanical performance of composite film	70:148	Grafting PEG on cellulose nanocrystals via polydopamine chemistry and the effects of PEG graft length on the mechanical performance of composite film.
34364549	0	18	theme	length	95:100	arg1	chemistry					56:64	polydopamine chemistry	43:64	polydopamine chemistry	43:64	Grafting PEG on cellulose nanocrystals via polydopamine chemistry and the effects of PEG graft length on the mechanical performance of composite film.
34364549	6	19	theme	PVA/CNC	1026:1032	arg1	composites					1034:1043	PVA/CNC composites	1026:1043	PVA/CNC composites	1026:1043	The longer PEG graft length yields high graft-matrix entanglement, forming a thicker rubbery coating layer that enhances the toughness of PVA/CNC composites.
34364549	6	20	theme	longer	892:897	arg1	length					909:914	The longer PEG graft length	888:914	The longer PEG graft length	888:914	The longer PEG graft length yields high graft-matrix entanglement, forming a thicker rubbery coating layer that enhances the toughness of PVA/CNC composites.
34364549	2	21	theme	length	507:512	arg1	effects					488:494	the effects	484:494	the effects of the PEG length on the properties of the PVA/CNC nanocomposites	484:560	The prepared CNC-PEGs were further used to reinforce the polyvinyl alcohol (PVA) at the loading of 0, 1, 3, 5, and 7 wt% in order to demonstrate the effects of the PEG length on the properties of the PVA/CNC nanocomposites.
34364549	1	22	theme	cellulose	166:174	arg1	CNCs					190:193	CNCs	190:193	CNCs	190:193	In this study, cellulose nanocrystals (CNCs) with PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa) were prepared via a polydopamine (PDA) mediated method in the aqueous solution.
34364549	1	22	theme	cellulose	166:174	arg1	nanocrystals					176:187	cellulose nanocrystals	166:187	cellulose nanocrystals (CNCs) with PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa)	166:257	In this study, cellulose nanocrystals (CNCs) with PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa) were prepared via a polydopamine (PDA) mediated method in the aqueous solution.
34364549	0	23	theme	cellulose	16:24	arg1	nanocrystals					26:37	cellulose nanocrystals	16:37	cellulose nanocrystals	16:37	Grafting PEG on cellulose nanocrystals via polydopamine chemistry and the effects of PEG graft length on the mechanical performance of composite film.
34364549	3	24	theme	simultaneous	600:611	arg1	improvements					613:624	simultaneous improvements	600:624	simultaneous improvements in stiffness and toughness	600:651	PEG surface modification resulted in simultaneous improvements in stiffness and toughness.
34364549	0	25	theme	mechanical	109:118	arg1	performance					120:130	the mechanical performance	105:130	the mechanical performance of composite film	105:148	Grafting PEG on cellulose nanocrystals via polydopamine chemistry and the effects of PEG graft length on the mechanical performance of composite film.
34364549	2	26	theme	PEG	503:505	arg1	length					507:512	the PEG length	499:512	the PEG length	499:512	The prepared CNC-PEGs were further used to reinforce the polyvinyl alcohol (PVA) at the loading of 0, 1, 3, 5, and 7 wt% in order to demonstrate the effects of the PEG length on the properties of the PVA/CNC nanocomposites.
34364549	2	27	theme	prepared	343:350	arg1	CNC-PEGs					352:359	The prepared CNC-PEGs	339:359	The prepared CNC-PEGs	339:359	The prepared CNC-PEGs were further used to reinforce the polyvinyl alcohol (PVA) at the loading of 0, 1, 3, 5, and 7 wt% in order to demonstrate the effects of the PEG length on the properties of the PVA/CNC nanocomposites.
34364549	1	28	theme	mediated	298:305	arg1	method					307:312	a polydopamine (PDA) mediated method	277:312	a polydopamine (PDA) mediated method in the aqueous solution	277:336	In this study, cellulose nanocrystals (CNCs) with PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa) were prepared via a polydopamine (PDA) mediated method in the aqueous solution.
34364549	6	29	theme	graft	903:907	arg1	length					909:914	The longer PEG graft length	888:914	The longer PEG graft length	888:914	The longer PEG graft length yields high graft-matrix entanglement, forming a thicker rubbery coating layer that enhances the toughness of PVA/CNC composites.
34364549	1	30	with	nanocrystals	176:187	arg1	grafts					205:210	PEG grafts	201:210	PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa)	201:257	In this study, cellulose nanocrystals (CNCs) with PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa) were prepared via a polydopamine (PDA) mediated method in the aqueous solution.
34364549	0	31	from	effects	74:80	arg1	performance					120:130	the mechanical performance	105:130	the mechanical performance of composite film	105:148	Grafting PEG on cellulose nanocrystals via polydopamine chemistry and the effects of PEG graft length on the mechanical performance of composite film.
34364549	5	32	theme	interface	868:876	arg1	adhesion					878:885	high interface adhesion	863:885	high interface adhesion	863:885	The shorter graft yields better enhancement in strength and stiffness, attributable to the high efficiency in the stress transfer and high interface adhesion.
34364549	5	33	theme	shorter	733:739	arg1	graft					741:745	The shorter graft	729:745	The shorter graft	729:745	The shorter graft yields better enhancement in strength and stiffness, attributable to the high efficiency in the stress transfer and high interface adhesion.
34364549	6	34	theme	PEG	899:901	arg1	length					909:914	The longer PEG graft length	888:914	The longer PEG graft length	888:914	The longer PEG graft length yields high graft-matrix entanglement, forming a thicker rubbery coating layer that enhances the toughness of PVA/CNC composites.
34364549	2	35	theme	PVA/CNC	539:545	arg1	nanocomposites					547:560	the PVA/CNC nanocomposites	535:560	the PVA/CNC nanocomposites	535:560	The prepared CNC-PEGs were further used to reinforce the polyvinyl alcohol (PVA) at the loading of 0, 1, 3, 5, and 7 wt% in order to demonstrate the effects of the PEG length on the properties of the PVA/CNC nanocomposites.
34364549	1	36	from	method	307:312	arg1	solution					329:336	the aqueous solution	317:336	the aqueous solution	317:336	In this study, cellulose nanocrystals (CNCs) with PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa) were prepared via a polydopamine (PDA) mediated method in the aqueous solution.
34364549	0	37	theme	film	145:148	arg1	performance					120:130	the mechanical performance	105:130	the mechanical performance of composite film	105:148	Grafting PEG on cellulose nanocrystals via polydopamine chemistry and the effects of PEG graft length on the mechanical performance of composite film.
34364549	2	38	from	effects	488:494	arg1	properties					521:530	the properties	517:530	the properties of the PVA/CNC nanocomposites	517:560	The prepared CNC-PEGs were further used to reinforce the polyvinyl alcohol (PVA) at the loading of 0, 1, 3, 5, and 7 wt% in order to demonstrate the effects of the PEG length on the properties of the PVA/CNC nanocomposites.
34364549	0	39	theme	polydopamine	43:54	arg1	chemistry					56:64	polydopamine chemistry	43:64	polydopamine chemistry	43:64	Grafting PEG on cellulose nanocrystals via polydopamine chemistry and the effects of PEG graft length on the mechanical performance of composite film.
34364549	0	40	theme	composite	135:143	arg1	film					145:148	composite film	135:148	composite film	135:148	Grafting PEG on cellulose nanocrystals via polydopamine chemistry and the effects of PEG graft length on the mechanical performance of composite film.
34364549	1	41	theme	aqueous	321:327	arg1	solution					329:336	the aqueous solution	317:336	the aqueous solution	317:336	In this study, cellulose nanocrystals (CNCs) with PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa) were prepared via a polydopamine (PDA) mediated method in the aqueous solution.
34364549	4	42	theme	noticeable	677:686	arg1	impacts					688:694	noticeable impacts	677:694	noticeable impacts	677:694	The graft lengths have noticeable impacts on the properties of composites.
34364549	5	43	theme	stress	843:848	arg1	transfer					850:857	the stress transfer	839:857	the stress transfer	839:857	The shorter graft yields better enhancement in strength and stiffness, attributable to the high efficiency in the stress transfer and high interface adhesion.
34364549	6	44	theme	coating	981:987	arg1	layer					989:993	a thicker rubbery coating layer	963:993	a thicker rubbery coating layer that enhances the toughness of PVA/CNC composites	963:1043	The longer PEG graft length yields high graft-matrix entanglement, forming a thicker rubbery coating layer that enhances the toughness of PVA/CNC composites.
34364549	6	45	theme	rubbery	973:979	arg1	layer					989:993	a thicker rubbery coating layer	963:993	a thicker rubbery coating layer that enhances the toughness of PVA/CNC composites	963:1043	The longer PEG graft length yields high graft-matrix entanglement, forming a thicker rubbery coating layer that enhances the toughness of PVA/CNC composites.
34364549	1	46	theme	PEG	201:203	arg1	grafts					205:210	PEG grafts	201:210	PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa)	201:257	In this study, cellulose nanocrystals (CNCs) with PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa) were prepared via a polydopamine (PDA) mediated method in the aqueous solution.
34364549	1	47	dep	lengths	223:229	arg1	lengths					223:229	various lengths	215:229	various lengths (1 k, 2 k, 5 k, and 10 kDa)	215:257	In this study, cellulose nanocrystals (CNCs) with PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa) were prepared via a polydopamine (PDA) mediated method in the aqueous solution.
34364549	1	47	dep	lengths	223:229	arg1	5 k					242:244	5 k	242:244	5 k	242:244	In this study, cellulose nanocrystals (CNCs) with PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa) were prepared via a polydopamine (PDA) mediated method in the aqueous solution.
34364549	1	47	dep	lengths	223:229	arg1	10 kDa					251:256	10 kDa	251:256	10 kDa	251:256	In this study, cellulose nanocrystals (CNCs) with PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa) were prepared via a polydopamine (PDA) mediated method in the aqueous solution.
34364549	1	47	dep	lengths	223:229	arg1	2 k					237:239	2 k	237:239	2 k	237:239	In this study, cellulose nanocrystals (CNCs) with PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa) were prepared via a polydopamine (PDA) mediated method in the aqueous solution.
34364549	1	47	dep	lengths	223:229	arg1	1 k					232:234	1 k	232:234	1 k	232:234	In this study, cellulose nanocrystals (CNCs) with PEG grafts of various lengths (1 k, 2 k, 5 k, and 10 kDa) were prepared via a polydopamine (PDA) mediated method in the aqueous solution.
34364549	4	48	theme	graft	658:662	arg1	lengths					664:670	The graft lengths	654:670	The graft lengths	654:670	The graft lengths have noticeable impacts on the properties of composites.
34364549	6	49	theme	thicker	965:971	arg1	layer					989:993	a thicker rubbery coating layer	963:993	a thicker rubbery coating layer that enhances the toughness of PVA/CNC composites	963:1043	The longer PEG graft length yields high graft-matrix entanglement, forming a thicker rubbery coating layer that enhances the toughness of PVA/CNC composites.
34364549	3	50	from	improvements	613:624	arg1	toughness					643:651	toughness	643:651	toughness	643:651	PEG surface modification resulted in simultaneous improvements in stiffness and toughness.
34364549	3	50	from	improvements	613:624	arg1	stiffness					629:637	stiffness	629:637	stiffness	629:637	PEG surface modification resulted in simultaneous improvements in stiffness and toughness.
34364549	2	51	used	used	374:377	arg2	CNC-PEGs					352:359	The prepared CNC-PEGs	339:359	The prepared CNC-PEGs	339:359	The prepared CNC-PEGs were further used to reinforce the polyvinyl alcohol (PVA) at the loading of 0, 1, 3, 5, and 7 wt% in order to demonstrate the effects of the PEG length on the properties of the PVA/CNC nanocomposites.
34364549	5	52	theme	high	863:866	arg1	adhesion					878:885	high interface adhesion	863:885	high interface adhesion	863:885	The shorter graft yields better enhancement in strength and stiffness, attributable to the high efficiency in the stress transfer and high interface adhesion.
32204063	8	0	with	compatible	1025:1034	arg1	compound					1067:1074	an antimicrobially active compound	1041:1074	an antimicrobially active compound	1041:1074	The new materials were moreover shown to be compatible with an antimicrobially active compound, which assures their efficiency in the treatment of highly colonized wounds.
32204063	4	1	theme	moisture	518:525	arg1	loss					527:530	reduced moisture loss	510:530	reduced moisture loss	510:530	To produce materials with reduced moisture loss, BC dressings were impregnated with a secondary hydrophilic component: alginate.
32204063	4	2	with	materials	495:503	arg1	loss					527:530	reduced moisture loss	510:530	reduced moisture loss	510:530	To produce materials with reduced moisture loss, BC dressings were impregnated with a secondary hydrophilic component: alginate.
32204063	7	3	theme	resultant	833:841	arg1	composite					843:851	The resultant composite	829:851	The resultant composite	829:851	The resultant composite possessed improved water-retention properties, providing a smooth dressing exchange as demonstrated by a wound-imitating model.
32204063	4	4	theme	BC	533:534	arg1	dressings					536:544	BC dressings	533:544	BC dressings	533:544	To produce materials with reduced moisture loss, BC dressings were impregnated with a secondary hydrophilic component: alginate.
32204063	7	5	theme	dressing	919:926	arg1	exchange					928:935	a smooth dressing exchange	910:935	a smooth dressing exchange as demonstrated by a wound-imitating model	910:978	The resultant composite possessed improved water-retention properties, providing a smooth dressing exchange as demonstrated by a wound-imitating model.
32204063	7	6	dep	exchange	928:935	arg1	demonstrated					940:951	demonstrated	940:951	demonstrated by a wound-imitating model	940:978	The resultant composite possessed improved water-retention properties, providing a smooth dressing exchange as demonstrated by a wound-imitating model.
32204063	0	7	with	impregnation	86:97	arg1	alginate					104:111	alginate	104:111	alginate	104:111	Fabrication of bacterial cellulose-based wound dressings with improved performance by impregnation with alginate.
32204063	3	8	theme	dressings´	360:369	arg1	ability					388:394	the dressings´ moisture-holding ability	356:394	the dressings´ moisture-holding ability	356:394	Improving the dressings´ moisture-holding ability facilitates its application and leads to an economically preferable extended wear time.
32204063	5	9	theme	pilot	693:697	arg1	equipment					705:713	pilot scale equipment	693:713	pilot scale equipment	693:713	The feasibility of an industrial fabrication of this composite was evaluated on pilot scale equipment.
32204063	2	10	theme	BC	282:283	arg1	dressing					285:292	the BC dressing	278:292	the BC dressing	278:292	The moist environment provided by the BC dressing is a key feature assuring efficient wound recovery.
32204063	5	11	theme	fabrication	646:656	arg1	feasibility					617:627	The feasibility	613:627	The feasibility of an industrial fabrication of this composite	613:674	The feasibility of an industrial fabrication of this composite was evaluated on pilot scale equipment.
32204063	5	12	theme	industrial	635:644	arg1	fabrication					646:656	an industrial fabrication	632:656	an industrial fabrication of this composite	632:674	The feasibility of an industrial fabrication of this composite was evaluated on pilot scale equipment.
32204063	7	13	theme	smooth	912:917	arg1	exchange					928:935	a smooth dressing exchange	910:935	a smooth dressing exchange as demonstrated by a wound-imitating model	910:978	The resultant composite possessed improved water-retention properties, providing a smooth dressing exchange as demonstrated by a wound-imitating model.
32204063	1	14	theme	efficient	168:176	arg1	materials					178:186	the most efficient materials	159:186	the most efficient materials	159:186	Bacterial cellulose (BC) hydrogels are among the most efficient materials already being used for the treatment of complex wounds.
32204063	4	15	theme	reduced	510:516	arg1	loss					527:530	reduced moisture loss	510:530	reduced moisture loss	510:530	To produce materials with reduced moisture loss, BC dressings were impregnated with a secondary hydrophilic component: alginate.
32204063	8	16	theme	active	1060:1065	arg1	compound					1067:1074	an antimicrobially active compound	1041:1074	an antimicrobially active compound	1041:1074	The new materials were moreover shown to be compatible with an antimicrobially active compound, which assures their efficiency in the treatment of highly colonized wounds.
32204063	3	17	theme	extended	464:471	arg1	time					478:481	an economically preferable extended wear time	437:481	an economically preferable extended wear time	437:481	Improving the dressings´ moisture-holding ability facilitates its application and leads to an economically preferable extended wear time.
32204063	6	18	theme	manufacturing	809:821	arg1	time					823:826	the manufacturing time	805:826	the manufacturing time	805:826	It was shown that the procedure can easily be scaled up without significantly increasing the manufacturing time.
32204063	4	19	theme	secondary	570:578	arg1	component					592:600	a secondary hydrophilic component	568:600	a secondary hydrophilic component	568:600	To produce materials with reduced moisture loss, BC dressings were impregnated with a secondary hydrophilic component: alginate.
32204063	5	20	theme	composite	666:674	arg1	fabrication					646:656	an industrial fabrication	632:656	an industrial fabrication of this composite	632:674	The feasibility of an industrial fabrication of this composite was evaluated on pilot scale equipment.
32204063	2	21	theme	key	299:301	arg1	environment					254:264	The moist environment	244:264	The moist environment provided by the BC dressing	244:292	The moist environment provided by the BC dressing is a key feature assuring efficient wound recovery.
32204063	2	21	theme	key	299:301	arg1	feature					303:309	a key feature	297:309	a key feature assuring efficient wound recovery	297:343	The moist environment provided by the BC dressing is a key feature assuring efficient wound recovery.
32204063	7	22	theme	water-retention	872:886	arg1	properties					888:897	improved water-retention properties	863:897	improved water-retention properties	863:897	The resultant composite possessed improved water-retention properties, providing a smooth dressing exchange as demonstrated by a wound-imitating model.
32204063	3	23	theme	moisture-holding	371:386	arg1	ability					388:394	the dressings´ moisture-holding ability	356:394	the dressings´ moisture-holding ability	356:394	Improving the dressings´ moisture-holding ability facilitates its application and leads to an economically preferable extended wear time.
32204063	8	24	theme	colonized	1135:1143	arg1	wounds					1145:1150	highly colonized wounds	1128:1150	highly colonized wounds	1128:1150	The new materials were moreover shown to be compatible with an antimicrobially active compound, which assures their efficiency in the treatment of highly colonized wounds.
32204063	1	25	theme	Bacterial	114:122	arg1	BC					135:136	BC	135:136	BC	135:136	Bacterial cellulose (BC) hydrogels are among the most efficient materials already being used for the treatment of complex wounds.
32204063	1	25	theme	Bacterial	114:122	arg1	cellulose					124:132	Bacterial cellulose	114:132	Bacterial cellulose (BC) hydrogels	114:147	Bacterial cellulose (BC) hydrogels are among the most efficient materials already being used for the treatment of complex wounds.
32204063	0	26	theme	bacterial	15:23	arg1	wound					41:45	bacterial cellulose-based wound	15:45	bacterial cellulose-based wound	15:45	Fabrication of bacterial cellulose-based wound dressings with improved performance by impregnation with alginate.
32204063	7	27	contain	possessed	853:861	arg2	properties					888:897	improved water-retention properties	863:897	improved water-retention properties	863:897	The resultant composite possessed improved water-retention properties, providing a smooth dressing exchange as demonstrated by a wound-imitating model.
32204063	7	27	contain	possessed	853:861	arg1	composite					843:851	The resultant composite	829:851	The resultant composite	829:851	The resultant composite possessed improved water-retention properties, providing a smooth dressing exchange as demonstrated by a wound-imitating model.
32204063	1	28	theme	cellulose	124:132	arg1	hydrogels					139:147	Bacterial cellulose (BC) hydrogels	114:147	Bacterial cellulose (BC) hydrogels	114:147	Bacterial cellulose (BC) hydrogels are among the most efficient materials already being used for the treatment of complex wounds.
32204063	4	29	theme	hydrophilic	580:590	arg1	component					592:600	a secondary hydrophilic component	568:600	a secondary hydrophilic component	568:600	To produce materials with reduced moisture loss, BC dressings were impregnated with a secondary hydrophilic component: alginate.
32204063	7	30	theme	improved	863:870	arg1	properties					888:897	improved water-retention properties	863:897	improved water-retention properties	863:897	The resultant composite possessed improved water-retention properties, providing a smooth dressing exchange as demonstrated by a wound-imitating model.
32204063	3	31	theme	wear	473:476	arg1	time					478:481	an economically preferable extended wear time	437:481	an economically preferable extended wear time	437:481	Improving the dressings´ moisture-holding ability facilitates its application and leads to an economically preferable extended wear time.
32204063	0	32	theme	wound	41:45	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of bacterial cellulose-based wound	0:45	Fabrication of bacterial cellulose-based wound dressings with improved performance by impregnation with alginate.
32204063	8	33	theme	wounds	1145:1150	arg1	treatment					1115:1123	the treatment	1111:1123	the treatment of highly colonized wounds	1111:1150	The new materials were moreover shown to be compatible with an antimicrobially active compound, which assures their efficiency in the treatment of highly colonized wounds.
32204063	0	34	theme	cellulose-based	25:39	arg1	wound					41:45	bacterial cellulose-based wound	15:45	bacterial cellulose-based wound	15:45	Fabrication of bacterial cellulose-based wound dressings with improved performance by impregnation with alginate.
32204063	0	35	theme	improved	62:69	arg1	performance					71:81	improved performance	62:81	improved performance	62:81	Fabrication of bacterial cellulose-based wound dressings with improved performance by impregnation with alginate.
32204063	5	36	theme	scale	699:703	arg1	equipment					705:713	pilot scale equipment	693:713	pilot scale equipment	693:713	The feasibility of an industrial fabrication of this composite was evaluated on pilot scale equipment.
32204063	3	37	theme	preferable	453:462	arg1	time					478:481	an economically preferable extended wear time	437:481	an economically preferable extended wear time	437:481	Improving the dressings´ moisture-holding ability facilitates its application and leads to an economically preferable extended wear time.
32204063	1	38	used	used	202:205	arg2	hydrogels					139:147	Bacterial cellulose (BC) hydrogels	114:147	Bacterial cellulose (BC) hydrogels	114:147	Bacterial cellulose (BC) hydrogels are among the most efficient materials already being used for the treatment of complex wounds.
32204063	2	39	theme	wound	330:334	arg1	recovery					336:343	efficient wound recovery	320:343	efficient wound recovery	320:343	The moist environment provided by the BC dressing is a key feature assuring efficient wound recovery.
32204063	7	40	theme	wound-imitating	958:972	arg1	model					974:978	a wound-imitating model	956:978	a wound-imitating model	956:978	The resultant composite possessed improved water-retention properties, providing a smooth dressing exchange as demonstrated by a wound-imitating model.
32204063	8	41	theme	new	985:987	arg1	materials					989:997	The new materials	981:997	The new materials	981:997	The new materials were moreover shown to be compatible with an antimicrobially active compound, which assures their efficiency in the treatment of highly colonized wounds.
32204063	8	41	theme	new	985:987	arg1	compatible					1025:1034	compatible	1025:1034	compatible	1025:1034	The new materials were moreover shown to be compatible with an antimicrobially active compound, which assures their efficiency in the treatment of highly colonized wounds.
32204063	2	42	theme	efficient	320:328	arg1	recovery					336:343	efficient wound recovery	320:343	efficient wound recovery	320:343	The moist environment provided by the BC dressing is a key feature assuring efficient wound recovery.
32204063	1	43	theme	complex	228:234	arg1	wounds					236:241	complex wounds	228:241	complex wounds	228:241	Bacterial cellulose (BC) hydrogels are among the most efficient materials already being used for the treatment of complex wounds.
32204063	2	44	theme	moist	248:252	arg1	environment					254:264	The moist environment	244:264	The moist environment provided by the BC dressing	244:292	The moist environment provided by the BC dressing is a key feature assuring efficient wound recovery.
32204063	2	44	theme	moist	248:252	arg1	feature					303:309	a key feature	297:309	a key feature assuring efficient wound recovery	297:343	The moist environment provided by the BC dressing is a key feature assuring efficient wound recovery.
32204063	1	45	theme	wounds	236:241	arg1	treatment					215:223	the treatment	211:223	the treatment of complex wounds	211:241	Bacterial cellulose (BC) hydrogels are among the most efficient materials already being used for the treatment of complex wounds.
32204063	8	46	from	efficiency	1097:1106	arg1	treatment					1115:1123	the treatment	1111:1123	the treatment of highly colonized wounds	1111:1150	The new materials were moreover shown to be compatible with an antimicrobially active compound, which assures their efficiency in the treatment of highly colonized wounds.
34280017	6	0	theme	wall-degrading	1128:1141	arg1	anti-M					1143:1148	cell wall-degrading anti-M	1123:1148	cell wall-degrading anti-M	1123:1148	We inserted a bacterial luciferase operon in an auxotrophic M. tuberculosis strain to develop a microtiter assay that allows proper evaluation of cell wall-degrading anti-M.
34280017	4	1	theme	M.	621:622	arg1	assay					666:670	an M. tuberculosis microtiter growth inhibition assay	618:670	an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays	618:826	In this study, we aimed to (i) develop an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays, (ii) explore anti-M.
34280017	10	2	theme	acid	1626:1629	arg1	barrier					1643:1649	the mycolic acid hydrophobic barrier	1614:1649	the mycolic acid hydrophobic barrier	1614:1649	Moreover, the most potent disruption of the mycolic acid hydrophobic barrier was obtained by the highly synergistic combination of LysB, α-amylase, and polysorbate 80.
34280017	8	3	theme	mycobacteriophage	1227:1243	arg1	[LysB					1267:1271	recombinant mycobacteriophage mycolic acid esterase [LysB	1215:1271	recombinant mycobacteriophage mycolic acid esterase [LysB	1215:1271	We assessed growth inhibition by enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB], fungal α-amylase, and human and chicken egg white lysozymes) and combinations thereof in the presence or absence of biopharmaceutically acceptable surfactant.
34280017	8	3	theme	mycobacteriophage	1227:1243	arg1	enzymes					1206:1212	enzymes	1206:1212	enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB]	1206:1272	We assessed growth inhibition by enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB], fungal α-amylase, and human and chicken egg white lysozymes) and combinations thereof in the presence or absence of biopharmaceutically acceptable surfactant.
34280017	4	4	theme	envelope	701:708	arg1	formation					710:718	undisturbed cell envelope formation	684:718	undisturbed cell envelope formation	684:718	In this study, we aimed to (i) develop an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays, (ii) explore anti-M.
34280017	12	5	theme	animal	2025:2030	arg1	models					2032:2037	animal models	2025:2037	animal models for active TB	2025:2051	Airway delivery of protein therapeutics has already been established and should be studied in animal models for active TB.
34280017	9	6	theme	potent	1500:1505	arg1	LysB					1478:1481	LysB	1478:1481	LysB	1478:1481	Our biosafety level 2 assay identified both LysB and lysozymes as potent M. tuberculosis inhibitors but only in the presence of surfactant.
34280017	9	6	theme	potent	1500:1505	arg1	inhibitors					1523:1532	potent M. tuberculosis inhibitors	1500:1532	potent M. tuberculosis inhibitors	1500:1532	Our biosafety level 2 assay identified both LysB and lysozymes as potent M. tuberculosis inhibitors but only in the presence of surfactant.
34280017	9	6	theme	potent	1500:1505	arg1	lysozymes					1487:1495	lysozymes	1487:1495	lysozymes	1487:1495	Our biosafety level 2 assay identified both LysB and lysozymes as potent M. tuberculosis inhibitors but only in the presence of surfactant.
34280017	8	7	theme	acid	1253:1256	arg1	[LysB					1267:1271	recombinant mycobacteriophage mycolic acid esterase [LysB	1215:1271	recombinant mycobacteriophage mycolic acid esterase [LysB	1215:1271	We assessed growth inhibition by enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB], fungal α-amylase, and human and chicken egg white lysozymes) and combinations thereof in the presence or absence of biopharmaceutically acceptable surfactant.
34280017	8	7	theme	acid	1253:1256	arg1	enzymes					1206:1212	enzymes	1206:1212	enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB]	1206:1272	We assessed growth inhibition by enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB], fungal α-amylase, and human and chicken egg white lysozymes) and combinations thereof in the presence or absence of biopharmaceutically acceptable surfactant.
34280017	2	8	theme	acid	337:340	arg1	layer-which					342:352	a mycolic acid layer-which	327:352	a mycolic acid layer-which	327:352	M. tuberculosis has a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics.
34280017	6	9	theme	luciferase	1001:1010	arg1	operon					1012:1017	a bacterial luciferase operon	989:1017	a bacterial luciferase operon	989:1017	We inserted a bacterial luciferase operon in an auxotrophic M. tuberculosis strain to develop a microtiter assay that allows proper evaluation of cell wall-degrading anti-M.
34280017	10	10	theme	barrier	1643:1649	arg1	disruption					1600:1609	the most potent disruption	1584:1609	the most potent disruption of the mycolic acid hydrophobic barrier	1584:1649	Moreover, the most potent disruption of the mycolic acid hydrophobic barrier was obtained by the highly synergistic combination of LysB, α-amylase, and polysorbate 80.
34280017	4	11	dep	M.	621:622	arg1	tuberculosis					624:635	tuberculosis	624:635	tuberculosis	624:635	In this study, we aimed to (i) develop an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays, (ii) explore anti-M.
34280017	5	12	theme	wall	879:882	arg1	enzymes					900:906	cell wall layer-degrading enzymes	874:906	cell wall layer-degrading enzymes	874:906	tuberculosis potency of cell wall layer-degrading enzymes, and (iii) investigate the concerted action of several such enzymes.
34280017	4	13	theme	undisturbed	684:694	arg1	formation					710:718	undisturbed cell envelope formation	684:718	undisturbed cell envelope formation	684:718	In this study, we aimed to (i) develop an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays, (ii) explore anti-M.
34280017	10	14	theme	synergistic	1678:1688	arg1	combination					1690:1700	the highly synergistic combination	1667:1700	the highly synergistic combination of LysB, α-amylase, and polysorbate 80	1667:1739	Moreover, the most potent disruption of the mycolic acid hydrophobic barrier was obtained by the highly synergistic combination of LysB, α-amylase, and polysorbate 80.
34280017	11	15	theme	antimycobacterial	1895:1911	arg1	enzymes					1922:1928	specifically tailored antimycobacterial (fusion) enzymes	1873:1928	specifically tailored antimycobacterial (fusion) enzymes	1873:1928	Synergistically acting cell wall-degrading enzymes are potently inhibiting M. tuberculosis, which sets the scene for the design of specifically tailored antimycobacterial (fusion) enzymes.
34280017	4	16	dep	develop	610:616	arg1	explore					834:840	explore	834:840	(ii) explore anti-M	829:847	In this study, we aimed to (i) develop an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays, (ii) explore anti-M.
34280017	4	16	dep	develop	610:616	arg1	i					607:607	i	607:607	i	607:607	In this study, we aimed to (i) develop an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays, (ii) explore anti-M.
34280017	5	17	theme	enzymes	900:906	arg1	potency					863:869	tuberculosis potency	850:869	tuberculosis potency of cell wall layer-degrading enzymes	850:906	tuberculosis potency of cell wall layer-degrading enzymes, and (iii) investigate the concerted action of several such enzymes.
34280017	4	18	theme	clumped	771:777	arg1	growth					795:800	typical clumped M. tuberculosis growth	763:800	typical clumped M. tuberculosis growth	763:800	In this study, we aimed to (i) develop an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays, (ii) explore anti-M.
34280017	2	19	theme	peptidoglycan	290:302	arg1	composite					260:268	composite	260:268	composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which	260:352	M. tuberculosis has a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics.
34280017	2	19	theme	peptidoglycan	290:302	arg1	peptidoglycan					290:302	peptidoglycan	290:302	peptidoglycan	290:302	M. tuberculosis has a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics.
34280017	2	19	theme	peptidoglycan	290:302	arg1	lipids					309:314	lipids	309:314	lipids	309:314	M. tuberculosis has a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics.
34280017	2	19	theme	peptidoglycan	290:302	arg1	polysaccharides					273:287	polysaccharides	273:287	polysaccharides	273:287	M. tuberculosis has a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics.
34280017	9	20	theme	surfactant	1562:1571	arg1	presence					1550:1557	the presence	1546:1557	the presence of surfactant	1546:1571	Our biosafety level 2 assay identified both LysB and lysozymes as potent M. tuberculosis inhibitors but only in the presence of surfactant.
34280017	4	21	dep	M.	779:780	arg1	tuberculosis					782:793	tuberculosis	782:793	tuberculosis	782:793	In this study, we aimed to (i) develop an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays, (ii) explore anti-M.
34280017	1	22	theme	Mycobacterium	158:170	arg1	tuberculosis					172:183	Mycobacterium tuberculosis	158:183	Mycobacterium tuberculosis	158:183	The major global health threat tuberculosis is caused by Mycobacterium tuberculosis.
34280017	6	23	theme	tuberculosis	1040:1051	arg1	strain					1053:1058	an auxotrophic M. tuberculosis strain	1022:1058	an auxotrophic M. tuberculosis strain	1022:1058	We inserted a bacterial luciferase operon in an auxotrophic M. tuberculosis strain to develop a microtiter assay that allows proper evaluation of cell wall-degrading anti-M.
34280017	5	24	theme	concerted	935:943	arg1	action					945:950	the concerted action	931:950	the concerted action of several such enzymes	931:974	tuberculosis potency of cell wall layer-degrading enzymes, and (iii) investigate the concerted action of several such enzymes.
34280017	6	25	theme	auxotrophic	1025:1035	arg1	strain					1053:1058	an auxotrophic M. tuberculosis strain	1022:1058	an auxotrophic M. tuberculosis strain	1022:1058	We inserted a bacterial luciferase operon in an auxotrophic M. tuberculosis strain to develop a microtiter assay that allows proper evaluation of cell wall-degrading anti-M.
34280017	12	26	theme	active	2043:2048	arg1	TB					2050:2051	active TB	2043:2051	active TB	2043:2051	Airway delivery of protein therapeutics has already been established and should be studied in animal models for active TB.
34280017	5	27	theme	such	963:966	arg1	enzymes					968:974	several such enzymes	955:974	several such enzymes	955:974	tuberculosis potency of cell wall layer-degrading enzymes, and (iii) investigate the concerted action of several such enzymes.
34280017	8	28	theme	acceptable	1411:1420	arg1	surfactant					1422:1431	biopharmaceutically acceptable surfactant	1391:1431	biopharmaceutically acceptable surfactant	1391:1431	We assessed growth inhibition by enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB], fungal α-amylase, and human and chicken egg white lysozymes) and combinations thereof in the presence or absence of biopharmaceutically acceptable surfactant.
34280017	10	29	theme	α-amylase	1711:1719	arg1	combination					1690:1700	the highly synergistic combination	1667:1700	the highly synergistic combination of LysB, α-amylase, and polysorbate 80	1667:1739	Moreover, the most potent disruption of the mycolic acid hydrophobic barrier was obtained by the highly synergistic combination of LysB, α-amylase, and polysorbate 80.
34280017	12	30	theme	therapeutics	1958:1969	arg1	delivery					1938:1945	Airway delivery	1931:1945	Airway delivery of protein therapeutics	1931:1969	Airway delivery of protein therapeutics has already been established and should be studied in animal models for active TB.
34280017	10	31	theme	LysB	1705:1708	arg1	combination					1690:1700	the highly synergistic combination	1667:1700	the highly synergistic combination of LysB, α-amylase, and polysorbate 80	1667:1739	Moreover, the most potent disruption of the mycolic acid hydrophobic barrier was obtained by the highly synergistic combination of LysB, α-amylase, and polysorbate 80.
34280017	5	32	theme	tuberculosis	850:861	arg1	potency					863:869	tuberculosis potency	850:869	tuberculosis potency of cell wall layer-degrading enzymes	850:906	tuberculosis potency of cell wall layer-degrading enzymes, and (iii) investigate the concerted action of several such enzymes.
34280017	1	33	theme	major	105:109	arg1	tuberculosis					132:143	The major global health threat tuberculosis	101:143	The major global health threat tuberculosis	101:143	The major global health threat tuberculosis is caused by Mycobacterium tuberculosis.
34280017	0	34	theme	Synergistic	15:25	arg1	Action					27:32	Synergistic Action	15:32	Synergistic Action of Cell Wall-Degrading Enzymes against Mycobacterium tuberculosis	15:98	Exploration of Synergistic Action of Cell Wall-Degrading Enzymes against Mycobacterium tuberculosis.
34280017	12	35	theme	Airway	1931:1936	arg1	delivery					1938:1945	Airway delivery	1931:1945	Airway delivery of protein therapeutics	1931:1969	Airway delivery of protein therapeutics has already been established and should be studied in animal models for active TB.
34280017	1	36	theme	health	118:123	arg1	tuberculosis					132:143	The major global health threat tuberculosis	101:143	The major global health threat tuberculosis	101:143	The major global health threat tuberculosis is caused by Mycobacterium tuberculosis.
34280017	11	37	theme	cell	1765:1768	arg1	enzymes					1785:1791	Synergistically acting cell wall-degrading enzymes	1742:1791	Synergistically acting cell wall-degrading enzymes	1742:1791	Synergistically acting cell wall-degrading enzymes are potently inhibiting M. tuberculosis, which sets the scene for the design of specifically tailored antimycobacterial (fusion) enzymes.
34280017	9	38	dep	M.	1507:1508	arg1	tuberculosis					1510:1521	tuberculosis	1510:1521	tuberculosis	1510:1521	Our biosafety level 2 assay identified both LysB and lysozymes as potent M. tuberculosis inhibitors but only in the presence of surfactant.
34280017	3	39	theme	-negative	470:478	arg1	infections					480:489	Gram-positive and -negative infections	452:489	Gram-positive and -negative infections	452:489	Given previous successes in treating Gram-positive and -negative infections with cell wall-degrading enzymes, we investigated such an approach for M. tuberculosis.
34280017	8	40	dep	presence	1368:1375	arg1	the					1364:1366	the	1364:1366	the	1364:1366	We assessed growth inhibition by enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB], fungal α-amylase, and human and chicken egg white lysozymes) and combinations thereof in the presence or absence of biopharmaceutically acceptable surfactant.
34280017	2	41	theme	M.	186:187	arg1	tuberculosis					189:200	M. tuberculosis	186:200	M. tuberculosis	186:200	M. tuberculosis has a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics.
34280017	11	42	theme	enzymes	1922:1928	arg1	design					1863:1868	the design	1859:1868	the design of specifically tailored antimycobacterial (fusion) enzymes	1859:1928	Synergistically acting cell wall-degrading enzymes are potently inhibiting M. tuberculosis, which sets the scene for the design of specifically tailored antimycobacterial (fusion) enzymes.
34280017	0	43	theme	Wall-Degrading	42:55	arg1	Enzymes					57:63	Cell Wall-Degrading Enzymes	37:63	Cell Wall-Degrading Enzymes	37:63	Exploration of Synergistic Action of Cell Wall-Degrading Enzymes against Mycobacterium tuberculosis.
34280017	2	44	theme	polysaccharides	273:287	arg1	composite					260:268	composite	260:268	composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which	260:352	M. tuberculosis has a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics.
34280017	2	44	theme	polysaccharides	273:287	arg1	peptidoglycan					290:302	peptidoglycan	290:302	peptidoglycan	290:302	M. tuberculosis has a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics.
34280017	2	44	theme	polysaccharides	273:287	arg1	lipids					309:314	lipids	309:314	lipids	309:314	M. tuberculosis has a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics.
34280017	2	44	theme	polysaccharides	273:287	arg1	polysaccharides					273:287	polysaccharides	273:287	polysaccharides	273:287	M. tuberculosis has a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics.
34280017	11	45	theme	fusion	1914:1919	arg1	enzymes					1922:1928	specifically tailored antimycobacterial (fusion) enzymes	1873:1928	specifically tailored antimycobacterial (fusion) enzymes	1873:1928	Synergistically acting cell wall-degrading enzymes are potently inhibiting M. tuberculosis, which sets the scene for the design of specifically tailored antimycobacterial (fusion) enzymes.
34280017	9	46	theme	level	1448:1452	arg1	assay					1456:1460	Our biosafety level 2 assay	1434:1460	Our biosafety level 2 assay	1434:1460	Our biosafety level 2 assay identified both LysB and lysozymes as potent M. tuberculosis inhibitors but only in the presence of surfactant.
34280017	2	47	theme	complex	208:214	arg1	envelope-a					221:230	a complex cell envelope-a	206:230	a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics	206:412	M. tuberculosis has a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics.
34280017	4	48	dep	explore	834:840	arg1	ii					830:831	ii	830:831	ii	830:831	In this study, we aimed to (i) develop an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays, (ii) explore anti-M.
34280017	6	49	theme	anti-M	1143:1148	arg1	evaluation					1109:1118	proper evaluation	1102:1118	proper evaluation of cell wall-degrading anti-M	1102:1148	We inserted a bacterial luciferase operon in an auxotrophic M. tuberculosis strain to develop a microtiter assay that allows proper evaluation of cell wall-degrading anti-M.
34280017	4	50	theme	growth	648:653	arg1	assay					666:670	an M. tuberculosis microtiter growth inhibition assay	618:670	an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays	618:826	In this study, we aimed to (i) develop an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays, (ii) explore anti-M.
34280017	10	51	theme	potent	1593:1598	arg1	disruption					1600:1609	the most potent disruption	1584:1609	the most potent disruption of the mycolic acid hydrophobic barrier	1584:1649	Moreover, the most potent disruption of the mycolic acid hydrophobic barrier was obtained by the highly synergistic combination of LysB, α-amylase, and polysorbate 80.
34280017	3	52	theme	M.	562:563	arg1	tuberculosis					565:576	M. tuberculosis	562:576	M. tuberculosis	562:576	Given previous successes in treating Gram-positive and -negative infections with cell wall-degrading enzymes, we investigated such an approach for M. tuberculosis.
34280017	6	53	theme	cell	1123:1126	arg1	anti-M					1143:1148	cell wall-degrading anti-M	1123:1148	cell wall-degrading anti-M	1123:1148	We inserted a bacterial luciferase operon in an auxotrophic M. tuberculosis strain to develop a microtiter assay that allows proper evaluation of cell wall-degrading anti-M.
34280017	10	54	theme	hydrophobic	1631:1641	arg1	barrier					1643:1649	the mycolic acid hydrophobic barrier	1614:1649	the mycolic acid hydrophobic barrier	1614:1649	Moreover, the most potent disruption of the mycolic acid hydrophobic barrier was obtained by the highly synergistic combination of LysB, α-amylase, and polysorbate 80.
34280017	10	55	theme	mycolic	1618:1624	arg1	barrier					1643:1649	the mycolic acid hydrophobic barrier	1614:1649	the mycolic acid hydrophobic barrier	1614:1649	Moreover, the most potent disruption of the mycolic acid hydrophobic barrier was obtained by the highly synergistic combination of LysB, α-amylase, and polysorbate 80.
34280017	2	56	theme	lipids	309:314	arg1	composite					260:268	composite	260:268	composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which	260:352	M. tuberculosis has a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics.
34280017	2	56	theme	lipids	309:314	arg1	peptidoglycan					290:302	peptidoglycan	290:302	peptidoglycan	290:302	M. tuberculosis has a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics.
34280017	2	56	theme	lipids	309:314	arg1	lipids					309:314	lipids	309:314	lipids	309:314	M. tuberculosis has a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics.
34280017	2	56	theme	lipids	309:314	arg1	polysaccharides					273:287	polysaccharides	273:287	polysaccharides	273:287	M. tuberculosis has a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics.
34280017	9	57	theme	M.	1507:1508	arg1	LysB					1478:1481	LysB	1478:1481	LysB	1478:1481	Our biosafety level 2 assay identified both LysB and lysozymes as potent M. tuberculosis inhibitors but only in the presence of surfactant.
34280017	9	57	theme	M.	1507:1508	arg1	inhibitors					1523:1532	potent M. tuberculosis inhibitors	1500:1532	potent M. tuberculosis inhibitors	1500:1532	Our biosafety level 2 assay identified both LysB and lysozymes as potent M. tuberculosis inhibitors but only in the presence of surfactant.
34280017	9	57	theme	M.	1507:1508	arg1	lysozymes					1487:1495	lysozymes	1487:1495	lysozymes	1487:1495	Our biosafety level 2 assay identified both LysB and lysozymes as potent M. tuberculosis inhibitors but only in the presence of surfactant.
34280017	8	58	theme	recombinant	1215:1225	arg1	[LysB					1267:1271	recombinant mycobacteriophage mycolic acid esterase [LysB	1215:1271	recombinant mycobacteriophage mycolic acid esterase [LysB	1215:1271	We assessed growth inhibition by enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB], fungal α-amylase, and human and chicken egg white lysozymes) and combinations thereof in the presence or absence of biopharmaceutically acceptable surfactant.
34280017	8	58	theme	recombinant	1215:1225	arg1	enzymes					1206:1212	enzymes	1206:1212	enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB]	1206:1272	We assessed growth inhibition by enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB], fungal α-amylase, and human and chicken egg white lysozymes) and combinations thereof in the presence or absence of biopharmaceutically acceptable surfactant.
34280017	4	59	theme	cell	696:699	arg1	formation					710:718	undisturbed cell envelope formation	684:718	undisturbed cell envelope formation	684:718	In this study, we aimed to (i) develop an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays, (ii) explore anti-M.
34280017	2	60	contain	has	202:204	arg2	envelope-a					221:230	a complex cell envelope-a	206:230	a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics	206:412	M. tuberculosis has a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics.
34280017	2	60	contain	has	202:204	arg1	tuberculosis					189:200	M. tuberculosis	186:200	M. tuberculosis	186:200	M. tuberculosis has a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics.
34280017	8	61	theme	mycolic	1245:1251	arg1	[LysB					1267:1271	recombinant mycobacteriophage mycolic acid esterase [LysB	1215:1271	recombinant mycobacteriophage mycolic acid esterase [LysB	1215:1271	We assessed growth inhibition by enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB], fungal α-amylase, and human and chicken egg white lysozymes) and combinations thereof in the presence or absence of biopharmaceutically acceptable surfactant.
34280017	8	61	theme	mycolic	1245:1251	arg1	enzymes					1206:1212	enzymes	1206:1212	enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB]	1206:1272	We assessed growth inhibition by enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB], fungal α-amylase, and human and chicken egg white lysozymes) and combinations thereof in the presence or absence of biopharmaceutically acceptable surfactant.
34280017	8	62	theme	human	1297:1301	arg1	lysozymes					1325:1333	human and chicken egg white lysozymes	1297:1333	human and chicken egg white lysozymes	1297:1333	We assessed growth inhibition by enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB], fungal α-amylase, and human and chicken egg white lysozymes) and combinations thereof in the presence or absence of biopharmaceutically acceptable surfactant.
34280017	2	63	theme	mycolic	329:335	arg1	layer-which					342:352	a mycolic acid layer-which	327:352	a mycolic acid layer-which	327:352	M. tuberculosis has a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics.
34280017	5	64	theme	cell	874:877	arg1	enzymes					900:906	cell wall layer-degrading enzymes	874:906	cell wall layer-degrading enzymes	874:906	tuberculosis potency of cell wall layer-degrading enzymes, and (iii) investigate the concerted action of several such enzymes.
34280017	8	65	theme	esterase	1258:1265	arg1	[LysB					1267:1271	recombinant mycobacteriophage mycolic acid esterase [LysB	1215:1271	recombinant mycobacteriophage mycolic acid esterase [LysB	1215:1271	We assessed growth inhibition by enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB], fungal α-amylase, and human and chicken egg white lysozymes) and combinations thereof in the presence or absence of biopharmaceutically acceptable surfactant.
34280017	8	65	theme	esterase	1258:1265	arg1	enzymes					1206:1212	enzymes	1206:1212	enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB]	1206:1272	We assessed growth inhibition by enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB], fungal α-amylase, and human and chicken egg white lysozymes) and combinations thereof in the presence or absence of biopharmaceutically acceptable surfactant.
34280017	6	66	theme	bacterial	991:999	arg1	operon					1012:1017	a bacterial luciferase operon	989:1017	a bacterial luciferase operon	989:1017	We inserted a bacterial luciferase operon in an auxotrophic M. tuberculosis strain to develop a microtiter assay that allows proper evaluation of cell wall-degrading anti-M.
34280017	8	67	theme	chicken	1307:1313	arg1	egg					1315:1317	chicken egg	1307:1317	chicken egg	1307:1317	We assessed growth inhibition by enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB], fungal α-amylase, and human and chicken egg white lysozymes) and combinations thereof in the presence or absence of biopharmaceutically acceptable surfactant.
34280017	5	68	theme	layer-degrading	884:898	arg1	enzymes					900:906	cell wall layer-degrading enzymes	874:906	cell wall layer-degrading enzymes	874:906	tuberculosis potency of cell wall layer-degrading enzymes, and (iii) investigate the concerted action of several such enzymes.
34280017	8	69	theme	white	1319:1323	arg1	lysozymes					1325:1333	human and chicken egg white lysozymes	1297:1333	human and chicken egg white lysozymes	1297:1333	We assessed growth inhibition by enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB], fungal α-amylase, and human and chicken egg white lysozymes) and combinations thereof in the presence or absence of biopharmaceutically acceptable surfactant.
34280017	1	70	theme	threat	125:130	arg1	tuberculosis					132:143	The major global health threat tuberculosis	101:143	The major global health threat tuberculosis	101:143	The major global health threat tuberculosis is caused by Mycobacterium tuberculosis.
34280017	8	71	theme	fungal	1275:1280	arg1	α-amylase					1282:1290	fungal α-amylase	1275:1290	fungal α-amylase	1275:1290	We assessed growth inhibition by enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB], fungal α-amylase, and human and chicken egg white lysozymes) and combinations thereof in the presence or absence of biopharmaceutically acceptable surfactant.
34280017	4	72	theme	results	752:758	arg1	invalidation					736:747	the invalidation	732:747	the invalidation of results	732:758	In this study, we aimed to (i) develop an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays, (ii) explore anti-M.
34280017	11	73	theme	tailored	1886:1893	arg1	enzymes					1922:1928	specifically tailored antimycobacterial (fusion) enzymes	1873:1928	specifically tailored antimycobacterial (fusion) enzymes	1873:1928	Synergistically acting cell wall-degrading enzymes are potently inhibiting M. tuberculosis, which sets the scene for the design of specifically tailored antimycobacterial (fusion) enzymes.
34280017	5	74	theme	several	955:961	arg1	enzymes					968:974	several such enzymes	955:974	several such enzymes	955:974	tuberculosis potency of cell wall layer-degrading enzymes, and (iii) investigate the concerted action of several such enzymes.
34280017	0	75	theme	Mycobacterium	73:85	arg1	tuberculosis					87:98	Mycobacterium tuberculosis	73:98	Mycobacterium tuberculosis	73:98	Exploration of Synergistic Action of Cell Wall-Degrading Enzymes against Mycobacterium tuberculosis.
34280017	11	76	theme	M.	1817:1818	arg1	tuberculosis					1820:1831	M. tuberculosis	1817:1831	M. tuberculosis	1817:1831	Synergistically acting cell wall-degrading enzymes are potently inhibiting M. tuberculosis, which sets the scene for the design of specifically tailored antimycobacterial (fusion) enzymes.
34280017	6	77	theme	M.	1037:1038	arg1	strain					1053:1058	an auxotrophic M. tuberculosis strain	1022:1058	an auxotrophic M. tuberculosis strain	1022:1058	We inserted a bacterial luciferase operon in an auxotrophic M. tuberculosis strain to develop a microtiter assay that allows proper evaluation of cell wall-degrading anti-M.
34280017	11	78	theme	wall-degrading	1770:1783	arg1	enzymes					1785:1791	Synergistically acting cell wall-degrading enzymes	1742:1791	Synergistically acting cell wall-degrading enzymes	1742:1791	Synergistically acting cell wall-degrading enzymes are potently inhibiting M. tuberculosis, which sets the scene for the design of specifically tailored antimycobacterial (fusion) enzymes.
34280017	4	79	theme	surfactant-free	805:819	arg1	assays					821:826	surfactant-free assays	805:826	surfactant-free assays	805:826	In this study, we aimed to (i) develop an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays, (ii) explore anti-M.
34280017	3	80	theme	wall-degrading	501:514	arg1	enzymes					516:522	cell wall-degrading enzymes	496:522	cell wall-degrading enzymes	496:522	Given previous successes in treating Gram-positive and -negative infections with cell wall-degrading enzymes, we investigated such an approach for M. tuberculosis.
34280017	5	81	theme	enzymes	968:974	arg1	action					945:950	the concerted action	931:950	the concerted action of several such enzymes	931:974	tuberculosis potency of cell wall layer-degrading enzymes, and (iii) investigate the concerted action of several such enzymes.
34280017	6	82	theme	proper	1102:1107	arg1	evaluation					1109:1118	proper evaluation	1102:1118	proper evaluation of cell wall-degrading anti-M	1102:1148	We inserted a bacterial luciferase operon in an auxotrophic M. tuberculosis strain to develop a microtiter assay that allows proper evaluation of cell wall-degrading anti-M.
34280017	8	83	theme	surfactant	1422:1431	arg1	absence					1380:1386	absence	1380:1386	absence	1380:1386	We assessed growth inhibition by enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB], fungal α-amylase, and human and chicken egg white lysozymes) and combinations thereof in the presence or absence of biopharmaceutically acceptable surfactant.
34280017	8	83	theme	surfactant	1422:1431	arg1	presence					1368:1375	presence	1368:1375	presence	1368:1375	We assessed growth inhibition by enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB], fungal α-amylase, and human and chicken egg white lysozymes) and combinations thereof in the presence or absence of biopharmaceutically acceptable surfactant.
34280017	0	84	theme	Action	27:32	arg1	Exploration					0:10	Exploration	0:10	Exploration of Synergistic Action of Cell Wall-Degrading Enzymes against Mycobacterium tuberculosis	0:98	Exploration of Synergistic Action of Cell Wall-Degrading Enzymes against Mycobacterium tuberculosis.
34280017	1	85	theme	global	111:116	arg1	tuberculosis					132:143	The major global health threat tuberculosis	101:143	The major global health threat tuberculosis	101:143	The major global health threat tuberculosis is caused by Mycobacterium tuberculosis.
34280017	3	86	theme	previous	421:428	arg1	successes					430:438	previous successes	421:438	previous successes in treating Gram-positive and -negative infections with cell wall-degrading enzymes	421:522	Given previous successes in treating Gram-positive and -negative infections with cell wall-degrading enzymes, we investigated such an approach for M. tuberculosis.
34280017	11	87	theme	acting	1758:1763	arg1	enzymes					1785:1791	Synergistically acting cell wall-degrading enzymes	1742:1791	Synergistically acting cell wall-degrading enzymes	1742:1791	Synergistically acting cell wall-degrading enzymes are potently inhibiting M. tuberculosis, which sets the scene for the design of specifically tailored antimycobacterial (fusion) enzymes.
34280017	5	88	dep	investigate	919:929	arg1	iii					914:916	iii	914:916	iii	914:916	tuberculosis potency of cell wall layer-degrading enzymes, and (iii) investigate the concerted action of several such enzymes.
34280017	10	89	theme	polysorbate	1726:1736	arg1	combination					1690:1700	the highly synergistic combination	1667:1700	the highly synergistic combination of LysB, α-amylase, and polysorbate 80	1667:1739	Moreover, the most potent disruption of the mycolic acid hydrophobic barrier was obtained by the highly synergistic combination of LysB, α-amylase, and polysorbate 80.
34280017	0	90	theme	Cell	37:40	arg1	Enzymes					57:63	Cell Wall-Degrading Enzymes	37:63	Cell Wall-Degrading Enzymes	37:63	Exploration of Synergistic Action of Cell Wall-Degrading Enzymes against Mycobacterium tuberculosis.
34280017	12	91	theme	protein	1950:1956	arg1	therapeutics					1958:1969	protein therapeutics	1950:1969	protein therapeutics	1950:1969	Airway delivery of protein therapeutics has already been established and should be studied in animal models for active TB.
34280017	6	92	theme	microtiter	1073:1082	arg1	assay					1084:1088	a microtiter assay	1071:1088	a microtiter assay that allows proper evaluation of cell wall-degrading anti-M	1071:1148	We inserted a bacterial luciferase operon in an auxotrophic M. tuberculosis strain to develop a microtiter assay that allows proper evaluation of cell wall-degrading anti-M.
34280017	3	93	theme	cell	496:499	arg1	enzymes					516:522	cell wall-degrading enzymes	496:522	cell wall-degrading enzymes	496:522	Given previous successes in treating Gram-positive and -negative infections with cell wall-degrading enzymes, we investigated such an approach for M. tuberculosis.
34280017	0	94	theme	Enzymes	57:63	arg1	Action					27:32	Synergistic Action	15:32	Synergistic Action of Cell Wall-Degrading Enzymes against Mycobacterium tuberculosis	15:98	Exploration of Synergistic Action of Cell Wall-Degrading Enzymes against Mycobacterium tuberculosis.
34280017	3	95	theme	Gram-positive	452:464	arg1	infections					480:489	Gram-positive and -negative infections	452:489	Gram-positive and -negative infections	452:489	Given previous successes in treating Gram-positive and -negative infections with cell wall-degrading enzymes, we investigated such an approach for M. tuberculosis.
34280017	9	96	theme	biosafety	1438:1446	arg1	assay					1456:1460	Our biosafety level 2 assay	1434:1460	Our biosafety level 2 assay	1434:1460	Our biosafety level 2 assay identified both LysB and lysozymes as potent M. tuberculosis inhibitors but only in the presence of surfactant.
34280017	4	97	dep	clumped	771:777	arg1	M.					779:780	M.	779:780	M.	779:780	In this study, we aimed to (i) develop an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays, (ii) explore anti-M.
34280017	4	97	dep	clumped	771:777	arg1	typical					763:769	typical	763:769	typical	763:769	In this study, we aimed to (i) develop an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays, (ii) explore anti-M.
34280017	4	98	theme	microtiter	637:646	arg1	assay					666:670	an M. tuberculosis microtiter growth inhibition assay	618:670	an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays	618:826	In this study, we aimed to (i) develop an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays, (ii) explore anti-M.
34280017	2	99	theme	cell	216:219	arg1	envelope-a					221:230	a complex cell envelope-a	206:230	a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics	206:412	M. tuberculosis has a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including a mycolic acid layer-which conveys pathogenicity but also protects against antibiotics.
34280017	8	100	theme	growth	1185:1190	arg1	inhibition					1192:1201	growth inhibition	1185:1201	growth inhibition by enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB], fungal α-amylase, and human and chicken egg white lysozymes)	1185:1334	We assessed growth inhibition by enzymes (recombinant mycobacteriophage mycolic acid esterase [LysB], fungal α-amylase, and human and chicken egg white lysozymes) and combinations thereof in the presence or absence of biopharmaceutically acceptable surfactant.
34280017	4	101	theme	inhibition	655:664	arg1	assay					666:670	an M. tuberculosis microtiter growth inhibition assay	618:670	an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays	618:826	In this study, we aimed to (i) develop an M. tuberculosis microtiter growth inhibition assay that allows undisturbed cell envelope formation to overcome the invalidation of results by typical clumped M. tuberculosis growth in surfactant-free assays, (ii) explore anti-M.
33579504	5	0	dep	response	1200:1207	arg1	i.e.					1181:1184	i.e.	1181:1184	i.e.	1181:1184	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	3	1	theme	polymer	805:811	arg1	ratio					813:817	the polymer ratio	801:817	the polymer ratio	801:817	The architecture and fiber diameter of the non-cross-linked nanofibrous scaffolds remained consistent irrespective of the polymer ratio under different electrospinning conditions, but the fiber diameter changed after cross-linking in association with the FC content.
33579504	2	2	theme	novel	458:462	arg1	scaffolds					484:492	novel bilayer nanofibrous scaffolds	458:492	novel bilayer nanofibrous scaffolds composed of fish collagen (FC) and poly(ε-caprolactone) (PCL)	458:554	In the present study, novel bilayer nanofibrous scaffolds composed of fish collagen (FC) and poly(ε-caprolactone) (PCL) were fabricated using electrospinning, with the covalent attachment of chitooligosaccharides (COS) via carbodiimide chemistry.
33579504	6	3	theme	swelling	1626:1633	arg1	assessment					1592:1601	an assessment	1589:1601	an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds	1589:1733	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	1	4	theme	biological	361:370	arg1	environment					372:382	the material and biological environment	344:382	the material and biological environment is of great interest in wound-healing applications	344:433	The development of tissue-engineered biodegradable artificial tissue substitutes with extracellular matrix-mimicking properties that govern the interaction between the material and biological environment is of great interest in wound-healing applications.
33579504	6	5	theme	nanofibrous	1713:1723	arg1	scaffolds					1725:1733	the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds	1664:1733	the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds	1664:1733	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	5	6	theme	dermal	1362:1367	arg1	NHDF-neo					1390:1397	normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells	1349:1427	NHDF-neo	1390:1397	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	3	7	theme	electrospinning	835:849	arg1	conditions					851:860	different electrospinning conditions	825:860	different electrospinning conditions	825:860	The architecture and fiber diameter of the non-cross-linked nanofibrous scaffolds remained consistent irrespective of the polymer ratio under different electrospinning conditions, but the fiber diameter changed after cross-linking in association with the FC content.
33579504	6	8	theme	FCP	1708:1710	arg1	scaffolds					1725:1733	the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds	1664:1733	the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds	1664:1733	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	6	9	contain	have	1755:1758	arg2	potential					1772:1780	significant potential	1760:1780	significant potential for use as tissue-engineered skin implants for rapid skin regeneration	1760:1851	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	6	9	contain	have	1755:1758	arg1	they					1750:1753	they	1750:1753	they	1750:1753	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	5	10	from	active	1327:1332	arg1	cells					1423:1427	normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells	1349:1427	cells	1423:1427	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	5	10	from	active	1327:1332	arg1	NHDF-neo					1390:1397	normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells	1349:1427	NHDF-neo	1390:1397	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	6	11	theme	FC/PCL	1700:1705	arg1	scaffolds					1725:1733	the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds	1664:1733	the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds	1664:1733	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	1	12	theme	matrix-mimicking	280:295	arg1	properties					297:306	extracellular matrix-mimicking properties	266:306	extracellular matrix-mimicking properties that govern the interaction between the material and biological environment is of great interest in wound-healing applications	266:433	The development of tissue-engineered biodegradable artificial tissue substitutes with extracellular matrix-mimicking properties that govern the interaction between the material and biological environment is of great interest in wound-healing applications.
33579504	6	13	theme	COS-containing	1677:1690	arg1	scaffolds					1725:1733	the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds	1664:1733	the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds	1664:1733	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	2	14	theme	chitooligosaccharides	627:647	arg1	attachment					613:622	the covalent attachment	600:622	the covalent attachment of chitooligosaccharides (COS) via carbodiimide chemistry	600:680	In the present study, novel bilayer nanofibrous scaffolds composed of fish collagen (FC) and poly(ε-caprolactone) (PCL) were fabricated using electrospinning, with the covalent attachment of chitooligosaccharides (COS) via carbodiimide chemistry.
33579504	5	15	theme	normal	1349:1354	arg1	NHDF-neo					1390:1397	normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells	1349:1427	NHDF-neo	1390:1397	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	6	16	theme	significant	1760:1770	arg1	potential					1772:1780	significant potential	1760:1780	significant potential for use as tissue-engineered skin implants for rapid skin regeneration	1760:1851	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	3	17	theme	FC	938:939	arg1	content					941:947	the FC content	934:947	the FC content	934:947	The architecture and fiber diameter of the non-cross-linked nanofibrous scaffolds remained consistent irrespective of the polymer ratio under different electrospinning conditions, but the fiber diameter changed after cross-linking in association with the FC content.
33579504	1	18	theme	biodegradable	217:229	arg1	tissue					242:247	tissue-engineered biodegradable artificial tissue	199:247	tissue-engineered biodegradable artificial tissue	199:247	The development of tissue-engineered biodegradable artificial tissue substitutes with extracellular matrix-mimicking properties that govern the interaction between the material and biological environment is of great interest in wound-healing applications.
33579504	5	19	theme	HaCaT	1404:1408	arg1	cells					1423:1427	normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells	1349:1427	cells	1423:1427	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	0	20	theme	cross-linked	98:109	arg1	chitooligosaccharides					111:131	covalently cross-linked chitooligosaccharides	87:131	covalently cross-linked chitooligosaccharides for full-thickness wound-healing applications	87:177	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite scaffolds with covalently cross-linked chitooligosaccharides for full-thickness wound-healing applications.
33579504	6	21	theme	skin	1835:1838	arg1	regeneration					1840:1851	rapid skin regeneration	1829:1851	rapid skin regeneration	1829:1851	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	1	22	theme	tissue	242:247	arg1	development					184:194	The development	180:194	The development of tissue-engineered biodegradable artificial tissue	180:247	The development of tissue-engineered biodegradable artificial tissue substitutes with extracellular matrix-mimicking properties that govern the interaction between the material and biological environment is of great interest in wound-healing applications.
33579504	5	23	theme	tissue-engineered	1476:1492	arg1	implant					1494:1500	a very effective tissue-engineered implant	1459:1500	a very effective tissue-engineered implant for full-thickness wound-healing applications	1459:1546	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	0	24	theme	wound-healing	152:164	arg1	applications					166:177	full-thickness wound-healing applications	137:177	full-thickness wound-healing applications	137:177	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite scaffolds with covalently cross-linked chitooligosaccharides for full-thickness wound-healing applications.
33579504	6	25	theme	skin	1811:1814	arg1	implants					1816:1823	tissue-engineered skin implants	1793:1823	tissue-engineered skin implants	1793:1823	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	0	26	theme	Electrospun	0:10	arg1	bio-composite					58:70	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite	0:70	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite	0:70	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite scaffolds with covalently cross-linked chitooligosaccharides for full-thickness wound-healing applications.
33579504	0	27	theme	bilayer	19:25	arg1	bio-composite					58:70	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite	0:70	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite	0:70	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite scaffolds with covalently cross-linked chitooligosaccharides for full-thickness wound-healing applications.
33579504	4	28	theme	Fourier-transform	950:966	arg1	analysis					990:997	Fourier-transform infrared spectroscopy analysis	950:997	Fourier-transform infrared spectroscopy analysis	950:997	Fourier-transform infrared spectroscopy analysis indicated that the blend of biomaterials was homogenous, with an increase in COS levels with increasing FC content in the nanofibrous scaffolds.
33579504	0	29	theme	fish	40:43	arg1	bio-composite					58:70	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite	0:70	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite	0:70	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite scaffolds with covalently cross-linked chitooligosaccharides for full-thickness wound-healing applications.
33579504	1	30	theme	wound-healing	408:420	arg1	applications					422:433	wound-healing applications	408:433	wound-healing applications	408:433	The development of tissue-engineered biodegradable artificial tissue substitutes with extracellular matrix-mimicking properties that govern the interaction between the material and biological environment is of great interest in wound-healing applications.
33579504	5	31	theme	high	1293:1296	arg1	content					1301:1307	high FC content	1293:1307	high FC content	1293:1307	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	3	32	theme	nanofibrous	743:753	arg1	scaffolds					755:763	the non-cross-linked nanofibrous scaffolds	722:763	the non-cross-linked nanofibrous scaffolds	722:763	The architecture and fiber diameter of the non-cross-linked nanofibrous scaffolds remained consistent irrespective of the polymer ratio under different electrospinning conditions, but the fiber diameter changed after cross-linking in association with the FC content.
33579504	4	33	theme	spectroscopy	977:988	arg1	analysis					990:997	Fourier-transform infrared spectroscopy analysis	950:997	Fourier-transform infrared spectroscopy analysis	950:997	Fourier-transform infrared spectroscopy analysis indicated that the blend of biomaterials was homogenous, with an increase in COS levels with increasing FC content in the nanofibrous scaffolds.
33579504	6	34	theme	empirical	1570:1578	arg1	results					1580:1586	these empirical results	1564:1586	these empirical results	1564:1586	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	2	35	theme	present	443:449	arg1	study					451:455	the present study	439:455	the present study	439:455	In the present study, novel bilayer nanofibrous scaffolds composed of fish collagen (FC) and poly(ε-caprolactone) (PCL) were fabricated using electrospinning, with the covalent attachment of chitooligosaccharides (COS) via carbodiimide chemistry.
33579504	5	36	theme	fibroblast‑neonatal	1369:1387	arg1	NHDF-neo					1390:1397	normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells	1349:1427	NHDF-neo	1390:1397	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	5	37	theme	wound-healing	1521:1533	arg1	applications					1535:1546	full-thickness wound-healing applications	1506:1546	full-thickness wound-healing applications	1506:1546	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	6	38	theme	mechanical	1640:1649	arg1	integrity					1651:1659	mechanical integrity	1640:1659	mechanical integrity	1640:1659	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	5	39	theme	cytocompatibility	1153:1169	arg1	analysis					1171:1178	cytocompatibility analysis	1153:1178	cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction)	1153:1259	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	3	40	link	non-cross-linked	726:741	arg1	scaffolds					755:763	the non-cross-linked nanofibrous scaffolds	722:763	the non-cross-linked nanofibrous scaffolds	722:763	The architecture and fiber diameter of the non-cross-linked nanofibrous scaffolds remained consistent irrespective of the polymer ratio under different electrospinning conditions, but the fiber diameter changed after cross-linking in association with the FC content.
33579504	3	41	theme	different	825:833	arg1	conditions					851:860	different electrospinning conditions	825:860	different electrospinning conditions	825:860	The architecture and fiber diameter of the non-cross-linked nanofibrous scaffolds remained consistent irrespective of the polymer ratio under different electrospinning conditions, but the fiber diameter changed after cross-linking in association with the FC content.
33579504	2	42	theme	bilayer	464:470	arg1	scaffolds					484:492	novel bilayer nanofibrous scaffolds	458:492	novel bilayer nanofibrous scaffolds composed of fish collagen (FC) and poly(ε-caprolactone) (PCL)	458:554	In the present study, novel bilayer nanofibrous scaffolds composed of fish collagen (FC) and poly(ε-caprolactone) (PCL) were fabricated using electrospinning, with the covalent attachment of chitooligosaccharides (COS) via carbodiimide chemistry.
33579504	4	43	from	content	1106:1112	arg1	scaffolds					1133:1141	the nanofibrous scaffolds	1117:1141	the nanofibrous scaffolds	1117:1141	Fourier-transform infrared spectroscopy analysis indicated that the blend of biomaterials was homogenous, with an increase in COS levels with increasing FC content in the nanofibrous scaffolds.
33579504	5	44	theme	human	1356:1360	arg1	NHDF-neo					1390:1397	normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells	1349:1427	NHDF-neo	1390:1397	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	4	45	theme	biomaterials	1027:1038	arg1	homogenous					1044:1053	homogenous	1044:1053	homogenous	1044:1053	Fourier-transform infrared spectroscopy analysis indicated that the blend of biomaterials was homogenous, with an increase in COS levels with increasing FC content in the nanofibrous scaffolds.
33579504	4	45	theme	biomaterials	1027:1038	arg1	blend					1018:1022	the blend	1014:1022	the blend of biomaterials	1014:1038	Fourier-transform infrared spectroscopy analysis indicated that the blend of biomaterials was homogenous, with an increase in COS levels with increasing FC content in the nanofibrous scaffolds.
33579504	5	46	from	cells	1423:1427	arg1	active					1327:1332	active	1327:1332	active	1327:1332	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	5	46	from	cells	1423:1427	arg1	scaffolds					1278:1286	the nanofibrous scaffolds	1262:1286	the nanofibrous scaffolds with high FC content	1262:1307	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	1	47	from	interest	396:403	arg1	applications					422:433	wound-healing applications	408:433	wound-healing applications	408:433	The development of tissue-engineered biodegradable artificial tissue substitutes with extracellular matrix-mimicking properties that govern the interaction between the material and biological environment is of great interest in wound-healing applications.
33579504	1	48	theme	extracellular	266:278	arg1	properties					297:306	extracellular matrix-mimicking properties	266:306	extracellular matrix-mimicking properties that govern the interaction between the material and biological environment is of great interest in wound-healing applications	266:433	The development of tissue-engineered biodegradable artificial tissue substitutes with extracellular matrix-mimicking properties that govern the interaction between the material and biological environment is of great interest in wound-healing applications.
33579504	3	49	dep	architecture	687:698	arg1	The					683:685	The	683:685	The	683:685	The architecture and fiber diameter of the non-cross-linked nanofibrous scaffolds remained consistent irrespective of the polymer ratio under different electrospinning conditions, but the fiber diameter changed after cross-linking in association with the FC content.
33579504	6	50	theme	FC-rich	1692:1698	arg1	scaffolds					1725:1733	the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds	1664:1733	the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds	1664:1733	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	5	51	dep	NHDF-neo	1390:1397	arg1	response					1337:1344	response	1337:1344	response	1337:1344	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	3	52	theme	fiber	871:875	arg1	diameter					877:884	the fiber diameter	867:884	the fiber diameter	867:884	The architecture and fiber diameter of the non-cross-linked nanofibrous scaffolds remained consistent irrespective of the polymer ratio under different electrospinning conditions, but the fiber diameter changed after cross-linking in association with the FC content.
33579504	5	53	theme	nanofibrous	1216:1226	arg1	scaffolds					1228:1236	the nanofibrous scaffolds	1212:1236	the nanofibrous scaffolds	1212:1236	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	6	54	theme	proposed	1668:1675	arg1	scaffolds					1725:1733	the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds	1664:1733	the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds	1664:1733	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	4	55	theme	COS	1076:1078	arg1	levels					1080:1085	COS levels	1076:1085	COS levels	1076:1085	Fourier-transform infrared spectroscopy analysis indicated that the blend of biomaterials was homogenous, with an increase in COS levels with increasing FC content in the nanofibrous scaffolds.
33579504	5	56	from	NHDF-neo	1390:1397	arg1	active					1327:1332	active	1327:1332	active	1327:1332	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	5	56	from	NHDF-neo	1390:1397	arg1	scaffolds					1278:1286	the nanofibrous scaffolds	1262:1286	the nanofibrous scaffolds with high FC content	1262:1307	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	4	57	from	increase	1064:1071	arg1	levels					1080:1085	COS levels	1076:1085	COS levels	1076:1085	Fourier-transform infrared spectroscopy analysis indicated that the blend of biomaterials was homogenous, with an increase in COS levels with increasing FC content in the nanofibrous scaffolds.
33579504	2	58	theme	carbodiimide	659:670	arg1	chemistry					672:680	carbodiimide chemistry	659:680	carbodiimide chemistry	659:680	In the present study, novel bilayer nanofibrous scaffolds composed of fish collagen (FC) and poly(ε-caprolactone) (PCL) were fabricated using electrospinning, with the covalent attachment of chitooligosaccharides (COS) via carbodiimide chemistry.
33579504	1	59	dep	environment	372:382	arg1	interest					396:403	great interest	390:403	great interest in wound-healing applications	390:433	The development of tissue-engineered biodegradable artificial tissue substitutes with extracellular matrix-mimicking properties that govern the interaction between the material and biological environment is of great interest in wound-healing applications.
33579504	5	60	theme	cellular	1191:1198	arg1	response					1200:1207	the cellular response	1187:1207	the cellular response to the nanofibrous scaffolds	1187:1236	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	3	61	theme	fiber	704:708	arg1	diameter					710:717	fiber diameter	704:717	fiber diameter	704:717	The architecture and fiber diameter of the non-cross-linked nanofibrous scaffolds remained consistent irrespective of the polymer ratio under different electrospinning conditions, but the fiber diameter changed after cross-linking in association with the FC content.
33579504	1	62	theme	tissue-engineered	199:215	arg1	tissue					242:247	tissue-engineered biodegradable artificial tissue	199:247	tissue-engineered biodegradable artificial tissue	199:247	The development of tissue-engineered biodegradable artificial tissue substitutes with extracellular matrix-mimicking properties that govern the interaction between the material and biological environment is of great interest in wound-healing applications.
33579504	5	63	with	scaffolds	1278:1286	arg1	content					1301:1307	high FC content	1293:1307	high FC content	1293:1307	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	5	64	theme	nanofibrous	1266:1276	arg1	active					1327:1332	active	1327:1332	active	1327:1332	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	5	64	theme	nanofibrous	1266:1276	arg1	scaffolds					1278:1286	the nanofibrous scaffolds	1262:1286	the nanofibrous scaffolds with high FC content	1262:1307	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	6	65	theme	scaffolds	1725:1733	arg1	integrity					1651:1659	mechanical integrity	1640:1659	mechanical integrity	1640:1659	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	6	65	theme	scaffolds	1725:1733	arg1	swelling					1626:1633	swelling	1626:1633	swelling	1626:1633	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	6	65	theme	scaffolds	1725:1733	arg1	hydrophilicity					1610:1623	hydrophilicity	1610:1623	hydrophilicity	1610:1623	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	1	66	theme	artificial	231:240	arg1	tissue					242:247	tissue-engineered biodegradable artificial tissue	199:247	tissue-engineered biodegradable artificial tissue	199:247	The development of tissue-engineered biodegradable artificial tissue substitutes with extracellular matrix-mimicking properties that govern the interaction between the material and biological environment is of great interest in wound-healing applications.
33579504	4	67	theme	nanofibrous	1121:1131	arg1	scaffolds					1133:1141	the nanofibrous scaffolds	1117:1141	the nanofibrous scaffolds	1117:1141	Fourier-transform infrared spectroscopy analysis indicated that the blend of biomaterials was homogenous, with an increase in COS levels with increasing FC content in the nanofibrous scaffolds.
33579504	5	68	theme	keratinocyte	1410:1421	arg1	cells					1423:1427	normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells	1349:1427	cells	1423:1427	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	0	69	theme	full-thickness	137:150	arg1	applications					166:177	full-thickness wound-healing applications	137:177	full-thickness wound-healing applications	137:177	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite scaffolds with covalently cross-linked chitooligosaccharides for full-thickness wound-healing applications.
33579504	6	70	theme	rapid	1829:1833	arg1	regeneration					1840:1851	rapid skin regeneration	1829:1851	rapid skin regeneration	1829:1851	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	2	71	theme	covalent	604:611	arg1	attachment					613:622	the covalent attachment	600:622	the covalent attachment of chitooligosaccharides (COS) via carbodiimide chemistry	600:680	In the present study, novel bilayer nanofibrous scaffolds composed of fish collagen (FC) and poly(ε-caprolactone) (PCL) were fabricated using electrospinning, with the covalent attachment of chitooligosaccharides (COS) via carbodiimide chemistry.
33579504	5	72	theme	effective	1466:1474	arg1	implant					1494:1500	a very effective tissue-engineered implant	1459:1500	a very effective tissue-engineered implant for full-thickness wound-healing applications	1459:1546	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	3	73	with	association	917:927	arg1	content					941:947	the FC content	934:947	the FC content	934:947	The architecture and fiber diameter of the non-cross-linked nanofibrous scaffolds remained consistent irrespective of the polymer ratio under different electrospinning conditions, but the fiber diameter changed after cross-linking in association with the FC content.
33579504	4	74	theme	FC	1103:1104	arg1	content					1106:1112	FC content	1103:1112	FC content in the nanofibrous scaffolds	1103:1141	Fourier-transform infrared spectroscopy analysis indicated that the blend of biomaterials was homogenous, with an increase in COS levels with increasing FC content in the nanofibrous scaffolds.
33579504	5	75	theme	implant	1494:1500	arg1	generation					1445:1454	the generation	1441:1454	the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications	1441:1546	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	2	76	theme	fish	506:509	arg1	FC					521:522	FC	521:522	FC	521:522	In the present study, novel bilayer nanofibrous scaffolds composed of fish collagen (FC) and poly(ε-caprolactone) (PCL) were fabricated using electrospinning, with the covalent attachment of chitooligosaccharides (COS) via carbodiimide chemistry.
33579504	2	76	theme	fish	506:509	arg1	collagen					511:518	fish collagen	506:518	fish collagen (FC)	506:523	In the present study, novel bilayer nanofibrous scaffolds composed of fish collagen (FC) and poly(ε-caprolactone) (PCL) were fabricated using electrospinning, with the covalent attachment of chitooligosaccharides (COS) via carbodiimide chemistry.
33579504	0	77	theme	porous	12:17	arg1	bio-composite					58:70	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite	0:70	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite	0:70	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite scaffolds with covalently cross-linked chitooligosaccharides for full-thickness wound-healing applications.
33579504	6	78	theme	tissue-engineered	1793:1809	arg1	implants					1816:1823	tissue-engineered skin implants	1793:1823	tissue-engineered skin implants	1793:1823	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	0	79	theme	nano-fibrous	27:38	arg1	bio-composite					58:70	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite	0:70	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite	0:70	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite scaffolds with covalently cross-linked chitooligosaccharides for full-thickness wound-healing applications.
33579504	1	80	theme	great	390:394	arg1	interest					396:403	great interest	390:403	great interest in wound-healing applications	390:433	The development of tissue-engineered biodegradable artificial tissue substitutes with extracellular matrix-mimicking properties that govern the interaction between the material and biological environment is of great interest in wound-healing applications.
33579504	6	81	dep	results	1580:1586	arg1	addition					1552:1559	addition	1552:1559	addition	1552:1559	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	3	82	theme	scaffolds	755:763	arg1	architecture					687:698	architecture	687:698	architecture	687:698	The architecture and fiber diameter of the non-cross-linked nanofibrous scaffolds remained consistent irrespective of the polymer ratio under different electrospinning conditions, but the fiber diameter changed after cross-linking in association with the FC content.
33579504	3	82	theme	scaffolds	755:763	arg1	diameter					710:717	fiber diameter	704:717	fiber diameter	704:717	The architecture and fiber diameter of the non-cross-linked nanofibrous scaffolds remained consistent irrespective of the polymer ratio under different electrospinning conditions, but the fiber diameter changed after cross-linking in association with the FC content.
33579504	0	83	theme	collagen/PCL	45:56	arg1	bio-composite					58:70	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite	0:70	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite	0:70	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite scaffolds with covalently cross-linked chitooligosaccharides for full-thickness wound-healing applications.
33579504	4	84	with	homogenous	1044:1053	arg1	increase					1064:1071	an increase	1061:1071	an increase in COS levels	1061:1085	Fourier-transform infrared spectroscopy analysis indicated that the blend of biomaterials was homogenous, with an increase in COS levels with increasing FC content in the nanofibrous scaffolds.
33579504	3	85	theme	non-cross-linked	726:741	arg1	scaffolds					755:763	the non-cross-linked nanofibrous scaffolds	722:763	the non-cross-linked nanofibrous scaffolds	722:763	The architecture and fiber diameter of the non-cross-linked nanofibrous scaffolds remained consistent irrespective of the polymer ratio under different electrospinning conditions, but the fiber diameter changed after cross-linking in association with the FC content.
33579504	5	86	theme	FC	1298:1299	arg1	content					1301:1307	high FC content	1293:1307	high FC content	1293:1307	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	0	87	link	cross-linked	98:109	arg1	chitooligosaccharides					111:131	covalently cross-linked chitooligosaccharides	87:131	covalently cross-linked chitooligosaccharides for full-thickness wound-healing applications	87:177	Electrospun porous bilayer nano-fibrous fish collagen/PCL bio-composite scaffolds with covalently cross-linked chitooligosaccharides for full-thickness wound-healing applications.
33579504	4	88	theme	infrared	968:975	arg1	analysis					990:997	Fourier-transform infrared spectroscopy analysis	950:997	Fourier-transform infrared spectroscopy analysis	950:997	Fourier-transform infrared spectroscopy analysis indicated that the blend of biomaterials was homogenous, with an increase in COS levels with increasing FC content in the nanofibrous scaffolds.
33579504	6	89	dep	hydrophilicity	1610:1623	arg1	the					1606:1608	the	1606:1608	the	1606:1608	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	5	90	theme	full-thickness	1506:1519	arg1	applications					1535:1546	full-thickness wound-healing applications	1506:1546	full-thickness wound-healing applications	1506:1546	Based on cytocompatibility analysis (i.e., the cellular response to the nanofibrous scaffolds and their interaction), the nanofibrous scaffolds with high FC content were functionally active in response to normal human dermal fibroblast‑neonatal (NHDF-neo) and HaCaT keratinocyte cells, leading to the generation of a very effective tissue-engineered implant for full-thickness wound-healing applications.
33579504	2	91	theme	nanofibrous	472:482	arg1	scaffolds					484:492	novel bilayer nanofibrous scaffolds	458:492	novel bilayer nanofibrous scaffolds composed of fish collagen (FC) and poly(ε-caprolactone) (PCL)	458:554	In the present study, novel bilayer nanofibrous scaffolds composed of fish collagen (FC) and poly(ε-caprolactone) (PCL) were fabricated using electrospinning, with the covalent attachment of chitooligosaccharides (COS) via carbodiimide chemistry.
33579504	6	92	theme	integrity	1651:1659	arg1	assessment					1592:1601	an assessment	1589:1601	an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds	1589:1733	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	6	93	theme	hydrophilicity	1610:1623	arg1	assessment					1592:1601	an assessment	1589:1601	an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds	1589:1733	In addition to these empirical results, an assessment of the hydrophilicity, swelling, and mechanical integrity of the proposed COS-containing FC-rich FC/PCL (FCP) nanofibrous scaffolds confirmed that they have significant potential for use as tissue-engineered skin implants for rapid skin regeneration.
33579504	1	94	theme	material	348:355	arg1	environment					372:382	the material and biological environment	344:382	the material and biological environment is of great interest in wound-healing applications	344:433	The development of tissue-engineered biodegradable artificial tissue substitutes with extracellular matrix-mimicking properties that govern the interaction between the material and biological environment is of great interest in wound-healing applications.
33673992	3	0	theme	resulting	556:564	arg1	composite					566:574	the resulting composite	552:574	the resulting composite	552:574	The incorporation of such nanoparticles enables us to modify both the strength and the humidity-dependent swelling direction of printed parts, offering a fourth-dimensional property to the resulting composite.
33673992	4	1	theme	functionalized	654:667	arg1	ε-polylysine					677:688	the functionalized peptide ε-polylysine	650:688	the functionalized peptide ε-polylysine	650:688	Antibacterial properties of the hydrogels were obtained by incorporating the functionalized peptide ε-polylysine, modified with the addition of a methacrylate group to ensure UV-immobilization.
33673992	4	2	theme	Antibacterial	577:589	arg1	properties					591:600	Antibacterial properties	577:600	Antibacterial properties of the hydrogels	577:617	Antibacterial properties of the hydrogels were obtained by incorporating the functionalized peptide ε-polylysine, modified with the addition of a methacrylate group to ensure UV-immobilization.
33673992	1	3	theme	antibacterial	154:166	arg1	hydrogels					221:229	antibacterial poly(N-isopropylacrylamide) nanocellulose-reinforced hydrogels	154:229	antibacterial poly(N-isopropylacrylamide) nanocellulose-reinforced hydrogels	154:229	We report on a procedure for the preparation, printing and curing of antibacterial poly(N-isopropylacrylamide) nanocellulose-reinforced hydrogels.
33673992	5	4	theme	properties	827:836	arg1	relevance					788:796	the relevance	784:796	the relevance of well-adapted viscoelastic properties of our material for 3D printing	784:868	We highlight the relevance of well-adapted viscoelastic properties of our material for 3D printing by direct ink writing of self-supporting complex structures reaching inclination angles of 45°.
33673992	6	5	theme	overall	1011:1017	arg1	composition					1023:1033	the overall ink composition	1007:1033	the overall ink composition	1007:1033	The addition of cellulose nanoparticles, the overall ink composition and the printing parameters strongly determine the resulting degree of orientation.
33673992	6	6	theme	orientation	1106:1116	arg1	degree					1096:1101	the resulting degree	1082:1101	the resulting degree of orientation	1082:1116	The addition of cellulose nanoparticles, the overall ink composition and the printing parameters strongly determine the resulting degree of orientation.
33673992	6	7	theme	resulting	1086:1094	arg1	degree					1096:1101	the resulting degree	1082:1101	the resulting degree of orientation	1082:1116	The addition of cellulose nanoparticles, the overall ink composition and the printing parameters strongly determine the resulting degree of orientation.
33673992	6	8	theme	printing	1043:1050	arg1	parameters					1052:1061	the printing parameters	1039:1061	the printing parameters	1039:1061	The addition of cellulose nanoparticles, the overall ink composition and the printing parameters strongly determine the resulting degree of orientation.
33673992	4	9	theme	peptide	669:675	arg1	ε-polylysine					677:688	the functionalized peptide ε-polylysine	650:688	the functionalized peptide ε-polylysine	650:688	Antibacterial properties of the hydrogels were obtained by incorporating the functionalized peptide ε-polylysine, modified with the addition of a methacrylate group to ensure UV-immobilization.
33673992	3	10	theme	such	388:391	arg1	nanoparticles					393:405	such nanoparticles	388:405	such nanoparticles	388:405	The incorporation of such nanoparticles enables us to modify both the strength and the humidity-dependent swelling direction of printed parts, offering a fourth-dimensional property to the resulting composite.
33673992	3	11	dep	strength	437:444	arg1	the					433:435	the	433:435	the	433:435	The incorporation of such nanoparticles enables us to modify both the strength and the humidity-dependent swelling direction of printed parts, offering a fourth-dimensional property to the resulting composite.
33673992	3	12	theme	nanoparticles	393:405	arg1	incorporation					371:383	The incorporation	367:383	The incorporation of such nanoparticles	367:405	The incorporation of such nanoparticles enables us to modify both the strength and the humidity-dependent swelling direction of printed parts, offering a fourth-dimensional property to the resulting composite.
33673992	6	13	theme	nanoparticles	992:1004	arg1	parameters					1052:1061	the printing parameters	1039:1061	the printing parameters	1039:1061	The addition of cellulose nanoparticles, the overall ink composition and the printing parameters strongly determine the resulting degree of orientation.
33673992	6	13	theme	nanoparticles	992:1004	arg1	composition					1023:1033	the overall ink composition	1007:1033	the overall ink composition	1007:1033	The addition of cellulose nanoparticles, the overall ink composition and the printing parameters strongly determine the resulting degree of orientation.
33673992	6	13	theme	nanoparticles	992:1004	arg1	addition					970:977	The addition	966:977	The addition of cellulose nanoparticles	966:1004	The addition of cellulose nanoparticles, the overall ink composition and the printing parameters strongly determine the resulting degree of orientation.
33673992	5	14	theme	direct	873:878	arg1	writing					884:890	direct ink writing	873:890	direct ink writing of self-supporting complex structures reaching inclination angles of 45°	873:963	We highlight the relevance of well-adapted viscoelastic properties of our material for 3D printing by direct ink writing of self-supporting complex structures reaching inclination angles of 45°.
33673992	1	15	dep	preparation	118:128	arg1	the					114:116	the	114:116	the	114:116	We report on a procedure for the preparation, printing and curing of antibacterial poly(N-isopropylacrylamide) nanocellulose-reinforced hydrogels.
33673992	3	16	theme	printed	495:501	arg1	parts					503:507	printed parts	495:507	printed parts	495:507	The incorporation of such nanoparticles enables us to modify both the strength and the humidity-dependent swelling direction of printed parts, offering a fourth-dimensional property to the resulting composite.
33673992	5	17	theme	self-supporting	895:909	arg1	structures					919:928	self-supporting complex structures	895:928	self-supporting complex structures reaching inclination angles of 45°	895:963	We highlight the relevance of well-adapted viscoelastic properties of our material for 3D printing by direct ink writing of self-supporting complex structures reaching inclination angles of 45°.
33673992	6	18	theme	cellulose	982:990	arg1	nanoparticles					992:1004	cellulose nanoparticles	982:1004	cellulose nanoparticles	982:1004	The addition of cellulose nanoparticles, the overall ink composition and the printing parameters strongly determine the resulting degree of orientation.
33673992	7	19	theme	three-dimensional	1206:1222	arg1	structures					1224:1233	complex three-dimensional structures	1198:1233	complex three-dimensional structures with programmable actuation	1198:1261	The achieved control over the anisotropic swelling properties paves the way to complex three-dimensional structures with programmable actuation.
33673992	3	20	theme	swelling	473:480	arg1	direction					482:490	the humidity-dependent swelling direction	450:490	the humidity-dependent swelling direction	450:490	The incorporation of such nanoparticles enables us to modify both the strength and the humidity-dependent swelling direction of printed parts, offering a fourth-dimensional property to the resulting composite.
33673992	0	21	theme	3D	0:1	arg1	printing					3:10	3D printing	0:10	3D printing of shape-morphing and antibacterial anisotropic nanocellulose hydrogels.	0:83	3D printing of shape-morphing and antibacterial anisotropic nanocellulose hydrogels.
33673992	2	22	theme	mechanical	344:353	arg1	properties					355:364	the resulting mechanical properties	330:364	the resulting mechanical properties	330:364	These composites present a highly anisotropic microstructure which allows to control and modulate the resulting mechanical properties.
33673992	0	23	theme	shape-morphing	15:28	arg1	hydrogels					74:82	shape-morphing and antibacterial anisotropic nanocellulose hydrogels	15:82	shape-morphing and antibacterial anisotropic nanocellulose hydrogels	15:82	3D printing of shape-morphing and antibacterial anisotropic nanocellulose hydrogels.
33673992	5	24	theme	complex	911:917	arg1	structures					919:928	self-supporting complex structures	895:928	self-supporting complex structures reaching inclination angles of 45°	895:963	We highlight the relevance of well-adapted viscoelastic properties of our material for 3D printing by direct ink writing of self-supporting complex structures reaching inclination angles of 45°.
33673992	2	25	theme	resulting	334:342	arg1	properties					355:364	the resulting mechanical properties	330:364	the resulting mechanical properties	330:364	These composites present a highly anisotropic microstructure which allows to control and modulate the resulting mechanical properties.
33673992	1	26	theme	nanocellulose-reinforced	196:219	arg1	hydrogels					221:229	antibacterial poly(N-isopropylacrylamide) nanocellulose-reinforced hydrogels	154:229	antibacterial poly(N-isopropylacrylamide) nanocellulose-reinforced hydrogels	154:229	We report on a procedure for the preparation, printing and curing of antibacterial poly(N-isopropylacrylamide) nanocellulose-reinforced hydrogels.
33673992	7	27	theme	complex	1198:1204	arg1	structures					1224:1233	complex three-dimensional structures	1198:1233	complex three-dimensional structures with programmable actuation	1198:1261	The achieved control over the anisotropic swelling properties paves the way to complex three-dimensional structures with programmable actuation.
33673992	1	28	theme	hydrogels	221:229	arg1	curing					144:149	curing	144:149	curing	144:149	We report on a procedure for the preparation, printing and curing of antibacterial poly(N-isopropylacrylamide) nanocellulose-reinforced hydrogels.
33673992	1	28	theme	hydrogels	221:229	arg1	printing					131:138	printing	131:138	printing	131:138	We report on a procedure for the preparation, printing and curing of antibacterial poly(N-isopropylacrylamide) nanocellulose-reinforced hydrogels.
33673992	1	28	theme	hydrogels	221:229	arg1	preparation					118:128	preparation	118:128	preparation	118:128	We report on a procedure for the preparation, printing and curing of antibacterial poly(N-isopropylacrylamide) nanocellulose-reinforced hydrogels.
33673992	0	29	theme	antibacterial	34:46	arg1	hydrogels					74:82	shape-morphing and antibacterial anisotropic nanocellulose hydrogels	15:82	shape-morphing and antibacterial anisotropic nanocellulose hydrogels	15:82	3D printing of shape-morphing and antibacterial anisotropic nanocellulose hydrogels.
33673992	5	30	theme	well-adapted	801:812	arg1	properties					827:836	well-adapted viscoelastic properties	801:836	well-adapted viscoelastic properties of our material	801:852	We highlight the relevance of well-adapted viscoelastic properties of our material for 3D printing by direct ink writing of self-supporting complex structures reaching inclination angles of 45°.
33673992	4	31	theme	methacrylate	723:734	arg1	group					736:740	a methacrylate group	721:740	a methacrylate group	721:740	Antibacterial properties of the hydrogels were obtained by incorporating the functionalized peptide ε-polylysine, modified with the addition of a methacrylate group to ensure UV-immobilization.
33673992	5	32	theme	viscoelastic	814:825	arg1	properties					827:836	well-adapted viscoelastic properties	801:836	well-adapted viscoelastic properties of our material	801:852	We highlight the relevance of well-adapted viscoelastic properties of our material for 3D printing by direct ink writing of self-supporting complex structures reaching inclination angles of 45°.
33673992	3	33	theme	parts	503:507	arg1	direction					482:490	the humidity-dependent swelling direction	450:490	the humidity-dependent swelling direction	450:490	The incorporation of such nanoparticles enables us to modify both the strength and the humidity-dependent swelling direction of printed parts, offering a fourth-dimensional property to the resulting composite.
33673992	3	33	theme	parts	503:507	arg1	strength					437:444	strength	437:444	strength	437:444	The incorporation of such nanoparticles enables us to modify both the strength and the humidity-dependent swelling direction of printed parts, offering a fourth-dimensional property to the resulting composite.
33673992	3	34	theme	humidity-dependent	454:471	arg1	direction					482:490	the humidity-dependent swelling direction	450:490	the humidity-dependent swelling direction	450:490	The incorporation of such nanoparticles enables us to modify both the strength and the humidity-dependent swelling direction of printed parts, offering a fourth-dimensional property to the resulting composite.
33673992	5	35	theme	ink	880:882	arg1	writing					884:890	direct ink writing	873:890	direct ink writing of self-supporting complex structures reaching inclination angles of 45°	873:963	We highlight the relevance of well-adapted viscoelastic properties of our material for 3D printing by direct ink writing of self-supporting complex structures reaching inclination angles of 45°.
33673992	5	36	theme	structures	919:928	arg1	writing					884:890	direct ink writing	873:890	direct ink writing of self-supporting complex structures reaching inclination angles of 45°	873:963	We highlight the relevance of well-adapted viscoelastic properties of our material for 3D printing by direct ink writing of self-supporting complex structures reaching inclination angles of 45°.
33673992	5	37	theme	material	845:852	arg1	properties					827:836	well-adapted viscoelastic properties	801:836	well-adapted viscoelastic properties of our material	801:852	We highlight the relevance of well-adapted viscoelastic properties of our material for 3D printing by direct ink writing of self-supporting complex structures reaching inclination angles of 45°.
33673992	0	38	theme	nanocellulose	60:72	arg1	hydrogels					74:82	shape-morphing and antibacterial anisotropic nanocellulose hydrogels	15:82	shape-morphing and antibacterial anisotropic nanocellulose hydrogels	15:82	3D printing of shape-morphing and antibacterial anisotropic nanocellulose hydrogels.
33673992	5	39	theme	3D	858:859	arg1	printing					861:868	3D printing	858:868	3D printing	858:868	We highlight the relevance of well-adapted viscoelastic properties of our material for 3D printing by direct ink writing of self-supporting complex structures reaching inclination angles of 45°.
33673992	0	40	theme	anisotropic	48:58	arg1	hydrogels					74:82	shape-morphing and antibacterial anisotropic nanocellulose hydrogels	15:82	shape-morphing and antibacterial anisotropic nanocellulose hydrogels	15:82	3D printing of shape-morphing and antibacterial anisotropic nanocellulose hydrogels.
33673992	4	41	theme	hydrogels	609:617	arg1	properties					591:600	Antibacterial properties	577:600	Antibacterial properties of the hydrogels	577:617	Antibacterial properties of the hydrogels were obtained by incorporating the functionalized peptide ε-polylysine, modified with the addition of a methacrylate group to ensure UV-immobilization.
33673992	6	42	theme	ink	1019:1021	arg1	composition					1023:1033	the overall ink composition	1007:1033	the overall ink composition	1007:1033	The addition of cellulose nanoparticles, the overall ink composition and the printing parameters strongly determine the resulting degree of orientation.
33673992	7	43	theme	programmable	1240:1251	arg1	actuation					1253:1261	programmable actuation	1240:1261	programmable actuation	1240:1261	The achieved control over the anisotropic swelling properties paves the way to complex three-dimensional structures with programmable actuation.
33673992	7	44	theme	achieved	1123:1130	arg1	control					1132:1138	The achieved control	1119:1138	The achieved control over the anisotropic swelling properties	1119:1179	The achieved control over the anisotropic swelling properties paves the way to complex three-dimensional structures with programmable actuation.
33673992	7	45	with	structures	1224:1233	arg1	actuation					1253:1261	programmable actuation	1240:1261	programmable actuation	1240:1261	The achieved control over the anisotropic swelling properties paves the way to complex three-dimensional structures with programmable actuation.
33673992	7	46	theme	anisotropic	1149:1159	arg1	properties					1170:1179	the anisotropic swelling properties	1145:1179	the anisotropic swelling properties	1145:1179	The achieved control over the anisotropic swelling properties paves the way to complex three-dimensional structures with programmable actuation.
33673992	3	47	theme	fourth-dimensional	521:538	arg1	property					540:547	a fourth-dimensional property	519:547	a fourth-dimensional property to the resulting composite	519:574	The incorporation of such nanoparticles enables us to modify both the strength and the humidity-dependent swelling direction of printed parts, offering a fourth-dimensional property to the resulting composite.
33673992	7	48	theme	swelling	1161:1168	arg1	properties					1170:1179	the anisotropic swelling properties	1145:1179	the anisotropic swelling properties	1145:1179	The achieved control over the anisotropic swelling properties paves the way to complex three-dimensional structures with programmable actuation.
33673992	5	49	theme	45°	961:963	arg1	angles					951:956	inclination angles	939:956	inclination angles of 45°	939:963	We highlight the relevance of well-adapted viscoelastic properties of our material for 3D printing by direct ink writing of self-supporting complex structures reaching inclination angles of 45°.
33673992	5	50	theme	inclination	939:949	arg1	angles					951:956	inclination angles	939:956	inclination angles of 45°	939:963	We highlight the relevance of well-adapted viscoelastic properties of our material for 3D printing by direct ink writing of self-supporting complex structures reaching inclination angles of 45°.
33673992	4	51	theme	group	736:740	arg1	addition					709:716	the addition	705:716	the addition of a methacrylate group to ensure UV-immobilization	705:768	Antibacterial properties of the hydrogels were obtained by incorporating the functionalized peptide ε-polylysine, modified with the addition of a methacrylate group to ensure UV-immobilization.
33673992	0	52	theme	hydrogels	74:82	arg1	printing					3:10	3D printing	0:10	3D printing of shape-morphing and antibacterial anisotropic nanocellulose hydrogels.	0:83	3D printing of shape-morphing and antibacterial anisotropic nanocellulose hydrogels.
33673992	2	53	theme	anisotropic	266:276	arg1	microstructure					278:291	a highly anisotropic microstructure	257:291	a highly anisotropic microstructure which allows to control and modulate the resulting mechanical properties	257:364	These composites present a highly anisotropic microstructure which allows to control and modulate the resulting mechanical properties.
32617941	0	0	theme	Infrared	79:86	arg1	Spectroscopy					88:99	Infrared Spectroscopy	79:99	Infrared Spectroscopy	79:99	Analysis of Plant Cell Walls by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy.
32617941	2	1	theme	samples	524:530	arg1	analysis					482:489	the nondestructive analysis	463:489	the nondestructive analysis of dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters using chemometric regression models	463:658	It is particularly well suited for the nondestructive analysis of dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters using chemometric regression models.
32617941	3	2	theme	processing	859:868	arg1	effects					834:840	the effects	830:840	the effects of experimental, processing, and environmental treatments	830:898	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	3	2	theme	processing	859:868	arg1	differences					928:938	genotypic and temporal differences	905:938	genotypic and temporal differences on chemical composition including changes in cell wall composition	905:1005	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	3	3	theme	treatments	889:898	arg1	effects					834:840	the effects	830:840	the effects of experimental, processing, and environmental treatments	830:898	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	3	3	theme	treatments	889:898	arg1	differences					928:938	genotypic and temporal differences	905:938	genotypic and temporal differences on chemical composition including changes in cell wall composition	905:1005	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	2	4	theme	plant	518:522	arg1	samples					524:530	dried and finely ground plant samples	494:530	dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters	494:622	It is particularly well suited for the nondestructive analysis of dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters using chemometric regression models.
32617941	5	5	theme	ATR-FTIR	1196:1203	arg1	spectra					1205:1211	ATR-FTIR spectra	1196:1211	ATR-FTIR spectra	1196:1211	Here we describe methods for cell wall sample preparation and generation of ATR-FTIR spectra, and suggest techniques for the statistical analysis and/or chemometric pattern recognition between the analyzed samples.
32617941	5	6	theme	cell	1149:1152	arg1	preparation					1166:1176	cell wall sample preparation	1149:1176	cell wall sample preparation	1149:1176	Here we describe methods for cell wall sample preparation and generation of ATR-FTIR spectra, and suggest techniques for the statistical analysis and/or chemometric pattern recognition between the analyzed samples.
32617941	3	7	theme	differences	928:938	arg1	comparison					816:825	multivariate comparison	803:825	multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition	803:1005	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	5	8	theme	chemometric	1273:1283	arg1	recognition					1293:1303	chemometric pattern recognition	1273:1303	chemometric pattern recognition	1273:1303	Here we describe methods for cell wall sample preparation and generation of ATR-FTIR spectra, and suggest techniques for the statistical analysis and/or chemometric pattern recognition between the analyzed samples.
32617941	2	9	theme	cell	565:568	arg1	wall					570:573	cell wall	565:573	cell wall	565:573	It is particularly well suited for the nondestructive analysis of dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters using chemometric regression models.
32617941	1	10	theme	traditional	375:385	arg1	methods					419:425	traditional wet chemical or chromatographic methods	375:425	traditional wet chemical or chromatographic methods	375:425	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods.
32617941	2	11	theme	regression	642:651	arg1	models					653:658	chemometric regression models	630:658	chemometric regression models	630:658	It is particularly well suited for the nondestructive analysis of dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters using chemometric regression models.
32617941	3	12	from	differences	928:938	arg1	composition					952:962	chemical composition	943:962	chemical composition including changes in cell wall composition	943:1005	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	4	13	theme	great	1022:1026	arg1	potential					1028:1036	great potential	1022:1036	great potential for using ATR-FTIR in the lignocellulosic biomass industry at a number of levels	1022:1117	There is thus great potential for using ATR-FTIR in the lignocellulosic biomass industry at a number of levels.
32617941	1	14	theme	wet	387:389	arg1	methods					419:425	traditional wet chemical or chromatographic methods	375:425	traditional wet chemical or chromatographic methods	375:425	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods.
32617941	2	15	theme	chemometric	630:640	arg1	models					653:658	chemometric regression models	630:658	chemometric regression models	630:658	It is particularly well suited for the nondestructive analysis of dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters using chemometric regression models.
32617941	3	16	theme	environmental	875:887	arg1	treatments					889:898	environmental treatments	875:898	environmental treatments	875:898	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	1	17	theme	chemical	391:398	arg1	methods					419:425	traditional wet chemical or chromatographic methods	375:425	traditional wet chemical or chromatographic methods	375:425	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods.
32617941	4	18	theme	levels	1112:1117	arg1	number					1102:1107	a number	1100:1107	a number of levels	1100:1117	There is thus great potential for using ATR-FTIR in the lignocellulosic biomass industry at a number of levels.
32617941	3	19	theme	cell	985:988	arg1	composition					995:1005	cell wall composition	985:1005	cell wall composition	985:1005	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	2	20	theme	dried	494:498	arg1	samples					524:530	dried and finely ground plant samples	494:530	dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters	494:622	It is particularly well suited for the nondestructive analysis of dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters using chemometric regression models.
32617941	4	21	theme	lignocellulosic	1064:1078	arg1	industry					1088:1095	the lignocellulosic biomass industry	1060:1095	the lignocellulosic biomass industry	1060:1095	There is thus great potential for using ATR-FTIR in the lignocellulosic biomass industry at a number of levels.
32617941	1	22	dep	Fourier	131:137	arg1	transform					139:147	transform	139:147	transform mid-infrared (ATR-FTIR) spectroscopy	139:184	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods.
32617941	5	23	theme	sample	1159:1164	arg1	preparation					1166:1176	cell wall sample preparation	1149:1176	cell wall sample preparation	1149:1176	Here we describe methods for cell wall sample preparation and generation of ATR-FTIR spectra, and suggest techniques for the statistical analysis and/or chemometric pattern recognition between the analyzed samples.
32617941	1	24	theme	chromatographic	403:417	arg1	methods					419:425	traditional wet chemical or chromatographic methods	375:425	traditional wet chemical or chromatographic methods	375:425	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods.
32617941	5	25	theme	statistical	1245:1255	arg1	analysis					1257:1264	the statistical analysis	1241:1264	the statistical analysis	1241:1264	Here we describe methods for cell wall sample preparation and generation of ATR-FTIR spectra, and suggest techniques for the statistical analysis and/or chemometric pattern recognition between the analyzed samples.
32617941	3	26	theme	multivariate	803:814	arg1	comparison					816:825	multivariate comparison	803:825	multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition	803:1005	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	3	27	theme	straightforward	774:788	arg1	approach					790:797	a straightforward approach	772:797	a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition	772:1005	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	3	28	theme	principal	733:741	arg1	analysis					753:760	principal component analysis	733:760	principal component analysis	733:760	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	1	29	theme	simple	296:301	arg1	preparation					310:320	only simple sample preparation	291:320	only simple sample preparation	291:320	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods.
32617941	3	30	from	comparison	816:825	arg1	composition					952:962	chemical composition	943:962	chemical composition including changes in cell wall composition	943:1005	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	0	31	theme	Cell	18:21	arg1	Walls					23:27	Plant Cell Walls	12:27	Plant Cell Walls	12:27	Analysis of Plant Cell Walls by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy.
32617941	1	32	theme	sample	303:308	arg1	preparation					310:320	only simple sample preparation	291:320	only simple sample preparation	291:320	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods.
32617941	2	33	theme	compositional	585:597	arg1	prediction					551:560	the subsequent prediction	536:560	the subsequent prediction of cell wall and other compositional or processing parameters	536:622	It is particularly well suited for the nondestructive analysis of dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters using chemometric regression models.
32617941	1	34	theme	Attenuated	102:111	arg1	Fourier					131:137	Attenuated total reflectance Fourier	102:137	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy	102:184	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods.
32617941	0	35	theme	Plant	12:16	arg1	Walls					23:27	Plant Cell Walls	12:27	Plant Cell Walls	12:27	Analysis of Plant Cell Walls by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy.
32617941	4	36	theme	biomass	1080:1086	arg1	industry					1088:1095	the lignocellulosic biomass industry	1060:1095	the lignocellulosic biomass industry	1060:1095	There is thus great potential for using ATR-FTIR in the lignocellulosic biomass industry at a number of levels.
32617941	2	37	theme	other	579:583	arg1	compositional					585:597	other compositional or processing parameters	579:622	compositional	585:597	It is particularly well suited for the nondestructive analysis of dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters using chemometric regression models.
32617941	3	38	theme	spectra	693:699	arg1	analysis					674:681	analysis	674:681	analysis of mid IR spectra by nonregression methods (e.g., principal component analysis)	674:761	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	1	39	theme	total	113:117	arg1	Fourier					131:137	Attenuated total reflectance Fourier	102:137	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy	102:184	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods.
32617941	3	40	theme	wall	990:993	arg1	composition					995:1005	cell wall composition	985:1005	cell wall composition	985:1005	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	1	41	theme	chemical	215:222	arg1	analysis					224:231	the chemical analysis	211:231	the chemical analysis of biological materials	211:255	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods.
32617941	2	42	theme	ground	511:516	arg1	samples					524:530	dried and finely ground plant samples	494:530	dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters	494:622	It is particularly well suited for the nondestructive analysis of dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters using chemometric regression models.
32617941	3	43	dep	analysis	753:760	arg1	e.g.					727:730	e.g.	727:730	e.g.	727:730	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	5	44	theme	analyzed	1317:1324	arg1	samples					1326:1332	the analyzed samples	1313:1332	the analyzed samples	1313:1332	Here we describe methods for cell wall sample preparation and generation of ATR-FTIR spectra, and suggest techniques for the statistical analysis and/or chemometric pattern recognition between the analyzed samples.
32617941	1	45	dep	applicable	196:205	arg1	high-throughput					347:361	high-throughput	347:361	high-throughput	347:361	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods.
32617941	1	45	dep	applicable	196:205	arg1	requires					282:289	requires	282:289	requires only simple sample preparation	282:320	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods.
32617941	1	45	dep	applicable	196:205	arg1	inexpensive					269:279	inexpensive	269:279	inexpensive	269:279	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods.
32617941	0	46	theme	Walls	23:27	arg1	Analysis					0:7	Analysis	0:7	Analysis of Plant Cell Walls by Attenuated Total Reflectance Fourier	0:67	Analysis of Plant Cell Walls by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy.
32617941	3	47	theme	effects	834:840	arg1	comparison					816:825	multivariate comparison	803:825	multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition	803:1005	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	2	48	theme	wall	570:573	arg1	prediction					551:560	the subsequent prediction	536:560	the subsequent prediction of cell wall and other compositional or processing parameters	536:622	It is particularly well suited for the nondestructive analysis of dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters using chemometric regression models.
32617941	3	49	theme	mid	686:688	arg1	spectra					693:699	mid IR spectra	686:699	mid IR spectra	686:699	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	2	50	theme	nondestructive	467:480	arg1	analysis					482:489	the nondestructive analysis	463:489	the nondestructive analysis of dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters using chemometric regression models	463:658	It is particularly well suited for the nondestructive analysis of dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters using chemometric regression models.
32617941	2	51	theme	subsequent	540:549	arg1	prediction					551:560	the subsequent prediction	536:560	the subsequent prediction of cell wall and other compositional or processing parameters	536:622	It is particularly well suited for the nondestructive analysis of dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters using chemometric regression models.
32617941	0	52	theme	Total	43:47	arg1	Fourier					61:67	Attenuated Total Reflectance Fourier	32:67	Attenuated Total Reflectance Fourier	32:67	Analysis of Plant Cell Walls by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy.
32617941	5	53	theme	wall	1154:1157	arg1	preparation					1166:1176	cell wall sample preparation	1149:1176	cell wall sample preparation	1149:1176	Here we describe methods for cell wall sample preparation and generation of ATR-FTIR spectra, and suggest techniques for the statistical analysis and/or chemometric pattern recognition between the analyzed samples.
32617941	3	54	theme	IR	690:691	arg1	spectra					693:699	mid IR spectra	686:699	mid IR spectra	686:699	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	1	55	theme	reflectance	119:129	arg1	Fourier					131:137	Attenuated total reflectance Fourier	102:137	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy	102:184	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods.
32617941	0	56	theme	Attenuated	32:41	arg1	Fourier					61:67	Attenuated Total Reflectance Fourier	32:67	Attenuated Total Reflectance Fourier	32:67	Analysis of Plant Cell Walls by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy.
32617941	3	57	from	effects	834:840	arg1	composition					952:962	chemical composition	943:962	chemical composition including changes in cell wall composition	943:1005	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	2	58	theme	processing	602:611	arg1	parameters					613:622	other compositional or processing parameters	579:622	parameters	613:622	It is particularly well suited for the nondestructive analysis of dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters using chemometric regression models.
32617941	5	59	theme	pattern	1285:1291	arg1	recognition					1293:1303	chemometric pattern recognition	1273:1303	chemometric pattern recognition	1273:1303	Here we describe methods for cell wall sample preparation and generation of ATR-FTIR spectra, and suggest techniques for the statistical analysis and/or chemometric pattern recognition between the analyzed samples.
32617941	3	60	theme	temporal	919:926	arg1	differences					928:938	genotypic and temporal differences	905:938	genotypic and temporal differences on chemical composition including changes in cell wall composition	905:1005	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	1	61	theme	biological	236:245	arg1	materials					247:255	biological materials	236:255	biological materials	236:255	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods.
32617941	3	62	from	composition	952:962	arg1	comparison					816:825	multivariate comparison	803:825	multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition	803:1005	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	3	63	theme	nonregression	704:716	arg1	methods					718:724	nonregression methods	704:724	nonregression methods (e.g., principal component analysis)	704:761	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	3	64	from	changes	974:980	arg1	composition					995:1005	cell wall composition	985:1005	cell wall composition	985:1005	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	1	65	theme	mid-infrared	149:160	arg1	spectroscopy					173:184	mid-infrared (ATR-FTIR) spectroscopy	149:184	mid-infrared (ATR-FTIR) spectroscopy	149:184	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods.
32617941	1	66	theme	materials	247:255	arg1	analysis					224:231	the chemical analysis	211:231	the chemical analysis of biological materials	211:255	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods.
32617941	5	67	theme	spectra	1205:1211	arg1	preparation					1166:1176	cell wall sample preparation	1149:1176	cell wall sample preparation	1149:1176	Here we describe methods for cell wall sample preparation and generation of ATR-FTIR spectra, and suggest techniques for the statistical analysis and/or chemometric pattern recognition between the analyzed samples.
32617941	5	67	theme	spectra	1205:1211	arg1	generation					1182:1191	generation	1182:1191	generation of ATR-FTIR spectra	1182:1211	Here we describe methods for cell wall sample preparation and generation of ATR-FTIR spectra, and suggest techniques for the statistical analysis and/or chemometric pattern recognition between the analyzed samples.
32617941	3	68	theme	genotypic	905:913	arg1	differences					928:938	genotypic and temporal differences	905:938	genotypic and temporal differences on chemical composition including changes in cell wall composition	905:1005	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	3	69	theme	experimental	845:856	arg1	effects					834:840	the effects	830:840	the effects of experimental, processing, and environmental treatments	830:898	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	3	69	theme	experimental	845:856	arg1	differences					928:938	genotypic and temporal differences	905:938	genotypic and temporal differences on chemical composition including changes in cell wall composition	905:1005	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	0	70	theme	Reflectance	49:59	arg1	Fourier					61:67	Attenuated Total Reflectance Fourier	32:67	Attenuated Total Reflectance Fourier	32:67	Analysis of Plant Cell Walls by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy.
32617941	3	71	theme	component	743:751	arg1	analysis					753:760	principal component analysis	733:760	principal component analysis	733:760	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32617941	1	72	theme	ATR-FTIR	163:170	arg1	spectroscopy					173:184	mid-infrared (ATR-FTIR) spectroscopy	149:184	mid-infrared (ATR-FTIR) spectroscopy	149:184	Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods.
32617941	3	73	theme	chemical	943:950	arg1	composition					952:962	chemical composition	943:962	chemical composition including changes in cell wall composition	943:1005	Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition.
32045528	0	0	theme	Distribution	70:81	arg1	Analysis					42:49	In Situ Analysis	34:49	In Situ Analysis of Structure, Size Distribution, and Dynamics of Prebiotic Oligosaccharides	34:125	13C-DOSY-TOSY NMR Correlation for In Situ Analysis of Structure, Size Distribution, and Dynamics of Prebiotic Oligosaccharides.
32045528	1	1	theme	nutritional	326:336	arg1	effects					360:366	numerous nutritional and health-beneficial effects	317:366	numerous nutritional and health-beneficial effects	317:366	Arabinoxylan oligosaccharides (AXOS) are a complex mixture of cereal derived, water-soluble prebiotics, obtained by enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects.
32045528	1	2	link	derived	197:203	arg1	prebiotics					220:229	cereal derived, water-soluble prebiotics	190:229	cereal derived, water-soluble prebiotics	190:229	Arabinoxylan oligosaccharides (AXOS) are a complex mixture of cereal derived, water-soluble prebiotics, obtained by enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects.
32045528	5	3	theme	compounds	1043:1051	arg1	mixtures					1008:1015	standard mixtures	999:1015	standard mixtures of aliphatic and aromatic compounds	999:1051	We have demonstrated this multifaceted 13C probed correlation strategy in standard mixtures of aliphatic and aromatic compounds, before implementing it on AXOS.
32045528	3	4	theme	AXOS	526:529	arg1	analysis					514:521	the in situ analysis	502:521	the in situ analysis of AXOS using a variety of state-of-the-art sensitivity-enhanced 13C-DOSY methods	502:603	Here we present the in situ analysis of AXOS using a variety of state-of-the-art sensitivity-enhanced 13C-DOSY methods, enabling virtual separation and identification of the components.
32045528	5	5	theme	aliphatic	1020:1028	arg1	compounds					1043:1051	aliphatic and aromatic compounds	1020:1051	aliphatic and aromatic compounds	1020:1051	We have demonstrated this multifaceted 13C probed correlation strategy in standard mixtures of aliphatic and aromatic compounds, before implementing it on AXOS.
32045528	0	6	theme	Size	65:68	arg1	Distribution					70:81	Size Distribution	65:81	Size Distribution	65:81	13C-DOSY-TOSY NMR Correlation for In Situ Analysis of Structure, Size Distribution, and Dynamics of Prebiotic Oligosaccharides.
32045528	4	7	theme	Ordered	752:758	arg1	SpectroscopY					760:771	Diffusion Ordered SpectroscopY	742:771	DOSY: Diffusion Ordered SpectroscopY	736:771	Three dimensional correlation plots displaying 13C diffusivity (DOSY: Diffusion Ordered SpectroscopY), relaxation parameters (TOSY: raTe of relaxation Ordered SpectrscopY), and chemical shift offer a unique way to elucidate the composition of mixtures.
32045528	1	8	theme	enzymatic	244:252	arg1	hydrolysis					254:263	enzymatic hydrolysis	244:263	enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects	244:366	Arabinoxylan oligosaccharides (AXOS) are a complex mixture of cereal derived, water-soluble prebiotics, obtained by enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects.
32045528	1	9	theme	health-beneficial	342:358	arg1	effects					360:366	numerous nutritional and health-beneficial effects	317:366	numerous nutritional and health-beneficial effects	317:366	Arabinoxylan oligosaccharides (AXOS) are a complex mixture of cereal derived, water-soluble prebiotics, obtained by enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects.
32045528	3	10	theme	sensitivity-enhanced	567:586	arg1	methods					597:603	state-of-the-art sensitivity-enhanced 13C-DOSY methods	550:603	state-of-the-art sensitivity-enhanced 13C-DOSY methods	550:603	Here we present the in situ analysis of AXOS using a variety of state-of-the-art sensitivity-enhanced 13C-DOSY methods, enabling virtual separation and identification of the components.
32045528	6	11	theme	3D-DOSY-TOSY	1092:1103	arg1	plots					1105:1109	These 3D-DOSY-TOSY plots	1086:1109	These 3D-DOSY-TOSY plots in combination with 2D-NMR correlation experiments	1086:1160	These 3D-DOSY-TOSY plots in combination with 2D-NMR correlation experiments offer unprecedented clarity for assigning chemical functions, molecular size distribution, and dynamics of oligosaccharide mixtures.
32045528	6	12	theme	size	1234:1237	arg1	distribution					1239:1250	molecular size distribution	1224:1250	molecular size distribution	1224:1250	These 3D-DOSY-TOSY plots in combination with 2D-NMR correlation experiments offer unprecedented clarity for assigning chemical functions, molecular size distribution, and dynamics of oligosaccharide mixtures.
32045528	4	13	theme	mixtures	915:922	arg1	composition					900:910	the composition	896:910	the composition of mixtures	896:922	Three dimensional correlation plots displaying 13C diffusivity (DOSY: Diffusion Ordered SpectroscopY), relaxation parameters (TOSY: raTe of relaxation Ordered SpectrscopY), and chemical shift offer a unique way to elucidate the composition of mixtures.
32045528	4	14	dep	Ordered	752:758	arg1	Diffusion					742:750	Diffusion	742:750	Diffusion	742:750	Three dimensional correlation plots displaying 13C diffusivity (DOSY: Diffusion Ordered SpectroscopY), relaxation parameters (TOSY: raTe of relaxation Ordered SpectrscopY), and chemical shift offer a unique way to elucidate the composition of mixtures.
32045528	3	15	theme	13C-DOSY	588:595	arg1	methods					597:603	state-of-the-art sensitivity-enhanced 13C-DOSY methods	550:603	state-of-the-art sensitivity-enhanced 13C-DOSY methods	550:603	Here we present the in situ analysis of AXOS using a variety of state-of-the-art sensitivity-enhanced 13C-DOSY methods, enabling virtual separation and identification of the components.
32045528	4	16	theme	relaxation	775:784	arg1	parameters					786:795	relaxation parameters	775:795	relaxation parameters (TOSY: raTe of relaxation Ordered SpectrscopY)	775:842	Three dimensional correlation plots displaying 13C diffusivity (DOSY: Diffusion Ordered SpectroscopY), relaxation parameters (TOSY: raTe of relaxation Ordered SpectrscopY), and chemical shift offer a unique way to elucidate the composition of mixtures.
32045528	4	17	theme	dimensional	678:688	arg1	plots					702:706	Three dimensional correlation plots	672:706	Three dimensional correlation plots displaying 13C diffusivity (DOSY: Diffusion Ordered SpectroscopY), relaxation parameters (TOSY: raTe of relaxation Ordered SpectrscopY), and chemical shift	672:862	Three dimensional correlation plots displaying 13C diffusivity (DOSY: Diffusion Ordered SpectroscopY), relaxation parameters (TOSY: raTe of relaxation Ordered SpectrscopY), and chemical shift offer a unique way to elucidate the composition of mixtures.
32045528	4	18	theme	SpectrscopY	831:841	arg1	raTe					804:807	raTe	804:807	TOSY: raTe of relaxation Ordered SpectrscopY	798:841	Three dimensional correlation plots displaying 13C diffusivity (DOSY: Diffusion Ordered SpectroscopY), relaxation parameters (TOSY: raTe of relaxation Ordered SpectrscopY), and chemical shift offer a unique way to elucidate the composition of mixtures.
32045528	5	19	theme	probed	968:973	arg1	strategy					987:994	this multifaceted 13C probed correlation strategy	946:994	this multifaceted 13C probed correlation strategy in standard mixtures of aliphatic and aromatic compounds	946:1051	We have demonstrated this multifaceted 13C probed correlation strategy in standard mixtures of aliphatic and aromatic compounds, before implementing it on AXOS.
32045528	6	20	theme	correlation	1138:1148	arg1	experiments					1150:1160	2D-NMR correlation experiments	1131:1160	2D-NMR correlation experiments	1131:1160	These 3D-DOSY-TOSY plots in combination with 2D-NMR correlation experiments offer unprecedented clarity for assigning chemical functions, molecular size distribution, and dynamics of oligosaccharide mixtures.
32045528	0	21	theme	Dynamics	88:95	arg1	Analysis					42:49	In Situ Analysis	34:49	In Situ Analysis of Structure, Size Distribution, and Dynamics of Prebiotic Oligosaccharides	34:125	13C-DOSY-TOSY NMR Correlation for In Situ Analysis of Structure, Size Distribution, and Dynamics of Prebiotic Oligosaccharides.
32045528	6	22	theme	assigning	1194:1202	arg1	functions					1213:1221	assigning chemical functions	1194:1221	assigning chemical functions	1194:1221	These 3D-DOSY-TOSY plots in combination with 2D-NMR correlation experiments offer unprecedented clarity for assigning chemical functions, molecular size distribution, and dynamics of oligosaccharide mixtures.
32045528	6	23	theme	molecular	1224:1232	arg1	distribution					1239:1250	molecular size distribution	1224:1250	molecular size distribution	1224:1250	These 3D-DOSY-TOSY plots in combination with 2D-NMR correlation experiments offer unprecedented clarity for assigning chemical functions, molecular size distribution, and dynamics of oligosaccharide mixtures.
32045528	3	24	theme	state-of-the-art	550:565	arg1	methods					597:603	state-of-the-art sensitivity-enhanced 13C-DOSY methods	550:603	state-of-the-art sensitivity-enhanced 13C-DOSY methods	550:603	Here we present the in situ analysis of AXOS using a variety of state-of-the-art sensitivity-enhanced 13C-DOSY methods, enabling virtual separation and identification of the components.
32045528	4	25	theme	13C	719:721	arg1	diffusivity					723:733	13C diffusivity	719:733	13C diffusivity (DOSY: Diffusion Ordered SpectroscopY)	719:772	Three dimensional correlation plots displaying 13C diffusivity (DOSY: Diffusion Ordered SpectroscopY), relaxation parameters (TOSY: raTe of relaxation Ordered SpectrscopY), and chemical shift offer a unique way to elucidate the composition of mixtures.
32045528	4	25	theme	13C	719:721	arg1	DOSY					736:739	DOSY	736:739	DOSY: Diffusion Ordered SpectroscopY	736:771	Three dimensional correlation plots displaying 13C diffusivity (DOSY: Diffusion Ordered SpectroscopY), relaxation parameters (TOSY: raTe of relaxation Ordered SpectrscopY), and chemical shift offer a unique way to elucidate the composition of mixtures.
32045528	0	26	theme	NMR	14:16	arg1	Correlation					18:28	13C-DOSY-TOSY NMR Correlation	0:28	13C-DOSY-TOSY NMR Correlation	0:28	13C-DOSY-TOSY NMR Correlation for In Situ Analysis of Structure, Size Distribution, and Dynamics of Prebiotic Oligosaccharides.
32045528	4	27	dep	parameters	786:795	arg1	TOSY					798:801	TOSY	798:801	TOSY: raTe of relaxation Ordered SpectrscopY	798:841	Three dimensional correlation plots displaying 13C diffusivity (DOSY: Diffusion Ordered SpectroscopY), relaxation parameters (TOSY: raTe of relaxation Ordered SpectrscopY), and chemical shift offer a unique way to elucidate the composition of mixtures.
32045528	5	28	theme	aromatic	1034:1041	arg1	compounds					1043:1051	aliphatic and aromatic compounds	1020:1051	aliphatic and aromatic compounds	1020:1051	We have demonstrated this multifaceted 13C probed correlation strategy in standard mixtures of aliphatic and aromatic compounds, before implementing it on AXOS.
32045528	1	29	theme	complex	171:177	arg1	mixture					179:185	a complex mixture	169:185	a complex mixture	169:185	Arabinoxylan oligosaccharides (AXOS) are a complex mixture of cereal derived, water-soluble prebiotics, obtained by enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects.
32045528	1	29	theme	complex	171:177	arg1	oligosaccharides					141:156	Arabinoxylan oligosaccharides	128:156	Arabinoxylan oligosaccharides (AXOS)	128:163	Arabinoxylan oligosaccharides (AXOS) are a complex mixture of cereal derived, water-soluble prebiotics, obtained by enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects.
32045528	0	30	theme	13C-DOSY-TOSY	0:12	arg1	Correlation					18:28	13C-DOSY-TOSY NMR Correlation	0:28	13C-DOSY-TOSY NMR Correlation	0:28	13C-DOSY-TOSY NMR Correlation for In Situ Analysis of Structure, Size Distribution, and Dynamics of Prebiotic Oligosaccharides.
32045528	1	31	theme	arabinoxylan	268:279	arg1	hydrolysis					254:263	enzymatic hydrolysis	244:263	enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects	244:366	Arabinoxylan oligosaccharides (AXOS) are a complex mixture of cereal derived, water-soluble prebiotics, obtained by enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects.
32045528	3	32	theme	virtual	615:621	arg1	separation					623:632	virtual separation	615:632	virtual separation	615:632	Here we present the in situ analysis of AXOS using a variety of state-of-the-art sensitivity-enhanced 13C-DOSY methods, enabling virtual separation and identification of the components.
32045528	2	33	theme	biomolecular	382:393	arg1	mixtures					395:402	Such complex biomolecular mixtures	369:402	Such complex biomolecular mixtures	369:402	Such complex biomolecular mixtures are notoriously difficult to characterize without initial physical fractionation.
32045528	2	34	theme	physical	462:469	arg1	fractionation					471:483	initial physical fractionation	454:483	initial physical fractionation	454:483	Such complex biomolecular mixtures are notoriously difficult to characterize without initial physical fractionation.
32045528	6	35	theme	mixtures	1285:1292	arg1	distribution					1239:1250	molecular size distribution	1224:1250	molecular size distribution	1224:1250	These 3D-DOSY-TOSY plots in combination with 2D-NMR correlation experiments offer unprecedented clarity for assigning chemical functions, molecular size distribution, and dynamics of oligosaccharide mixtures.
32045528	6	35	theme	mixtures	1285:1292	arg1	dynamics					1257:1264	dynamics	1257:1264	dynamics	1257:1264	These 3D-DOSY-TOSY plots in combination with 2D-NMR correlation experiments offer unprecedented clarity for assigning chemical functions, molecular size distribution, and dynamics of oligosaccharide mixtures.
32045528	6	35	theme	mixtures	1285:1292	arg1	functions					1213:1221	assigning chemical functions	1194:1221	assigning chemical functions	1194:1221	These 3D-DOSY-TOSY plots in combination with 2D-NMR correlation experiments offer unprecedented clarity for assigning chemical functions, molecular size distribution, and dynamics of oligosaccharide mixtures.
32045528	0	36	theme	Oligosaccharides	110:125	arg1	Structure					54:62	Structure	54:62	Structure	54:62	13C-DOSY-TOSY NMR Correlation for In Situ Analysis of Structure, Size Distribution, and Dynamics of Prebiotic Oligosaccharides.
32045528	0	36	theme	Oligosaccharides	110:125	arg1	Distribution					70:81	Size Distribution	65:81	Size Distribution	65:81	13C-DOSY-TOSY NMR Correlation for In Situ Analysis of Structure, Size Distribution, and Dynamics of Prebiotic Oligosaccharides.
32045528	0	36	theme	Oligosaccharides	110:125	arg1	Dynamics					88:95	Dynamics	88:95	Dynamics	88:95	13C-DOSY-TOSY NMR Correlation for In Situ Analysis of Structure, Size Distribution, and Dynamics of Prebiotic Oligosaccharides.
32045528	5	37	theme	correlation	975:985	arg1	strategy					987:994	this multifaceted 13C probed correlation strategy	946:994	this multifaceted 13C probed correlation strategy in standard mixtures of aliphatic and aromatic compounds	946:1051	We have demonstrated this multifaceted 13C probed correlation strategy in standard mixtures of aliphatic and aromatic compounds, before implementing it on AXOS.
32045528	3	38	theme	methods	597:603	arg1	methods					597:603	state-of-the-art sensitivity-enhanced 13C-DOSY methods	550:603	state-of-the-art sensitivity-enhanced 13C-DOSY methods	550:603	Here we present the in situ analysis of AXOS using a variety of state-of-the-art sensitivity-enhanced 13C-DOSY methods, enabling virtual separation and identification of the components.
32045528	3	38	theme	methods	597:603	arg1	variety					539:545	a variety	537:545	a variety of state-of-the-art sensitivity-enhanced 13C-DOSY methods	537:603	Here we present the in situ analysis of AXOS using a variety of state-of-the-art sensitivity-enhanced 13C-DOSY methods, enabling virtual separation and identification of the components.
32045528	4	39	theme	correlation	690:700	arg1	plots					702:706	Three dimensional correlation plots	672:706	Three dimensional correlation plots displaying 13C diffusivity (DOSY: Diffusion Ordered SpectroscopY), relaxation parameters (TOSY: raTe of relaxation Ordered SpectrscopY), and chemical shift	672:862	Three dimensional correlation plots displaying 13C diffusivity (DOSY: Diffusion Ordered SpectroscopY), relaxation parameters (TOSY: raTe of relaxation Ordered SpectrscopY), and chemical shift offer a unique way to elucidate the composition of mixtures.
32045528	2	40	theme	initial	454:460	arg1	fractionation					471:483	initial physical fractionation	454:483	initial physical fractionation	454:483	Such complex biomolecular mixtures are notoriously difficult to characterize without initial physical fractionation.
32045528	0	41	theme	Prebiotic	100:108	arg1	Oligosaccharides					110:125	Prebiotic Oligosaccharides	100:125	Prebiotic Oligosaccharides	100:125	13C-DOSY-TOSY NMR Correlation for In Situ Analysis of Structure, Size Distribution, and Dynamics of Prebiotic Oligosaccharides.
32045528	1	42	theme	cereal	190:195	arg1	prebiotics					220:229	cereal derived, water-soluble prebiotics	190:229	cereal derived, water-soluble prebiotics	190:229	Arabinoxylan oligosaccharides (AXOS) are a complex mixture of cereal derived, water-soluble prebiotics, obtained by enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects.
32045528	5	43	theme	13C	964:966	arg1	strategy					987:994	this multifaceted 13C probed correlation strategy	946:994	this multifaceted 13C probed correlation strategy in standard mixtures of aliphatic and aromatic compounds	946:1051	We have demonstrated this multifaceted 13C probed correlation strategy in standard mixtures of aliphatic and aromatic compounds, before implementing it on AXOS.
32045528	6	44	theme	chemical	1204:1211	arg1	functions					1213:1221	assigning chemical functions	1194:1221	assigning chemical functions	1194:1221	These 3D-DOSY-TOSY plots in combination with 2D-NMR correlation experiments offer unprecedented clarity for assigning chemical functions, molecular size distribution, and dynamics of oligosaccharide mixtures.
32045528	1	45	theme	derived	197:203	arg1	prebiotics					220:229	cereal derived, water-soluble prebiotics	190:229	cereal derived, water-soluble prebiotics	190:229	Arabinoxylan oligosaccharides (AXOS) are a complex mixture of cereal derived, water-soluble prebiotics, obtained by enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects.
32045528	0	46	theme	In	34:35	arg1	Analysis					42:49	In Situ Analysis	34:49	In Situ Analysis of Structure, Size Distribution, and Dynamics of Prebiotic Oligosaccharides	34:125	13C-DOSY-TOSY NMR Correlation for In Situ Analysis of Structure, Size Distribution, and Dynamics of Prebiotic Oligosaccharides.
32045528	6	47	with	combination	1114:1124	arg1	experiments					1150:1160	2D-NMR correlation experiments	1131:1160	2D-NMR correlation experiments	1131:1160	These 3D-DOSY-TOSY plots in combination with 2D-NMR correlation experiments offer unprecedented clarity for assigning chemical functions, molecular size distribution, and dynamics of oligosaccharide mixtures.
32045528	1	48	theme	dietary	293:299	arg1	fibers					301:306	dietary fibers	293:306	dietary fibers exerting numerous nutritional and health-beneficial effects	293:366	Arabinoxylan oligosaccharides (AXOS) are a complex mixture of cereal derived, water-soluble prebiotics, obtained by enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects.
32045528	6	49	from	plots	1105:1109	arg1	combination					1114:1124	combination	1114:1124	combination with 2D-NMR correlation experiments	1114:1160	These 3D-DOSY-TOSY plots in combination with 2D-NMR correlation experiments offer unprecedented clarity for assigning chemical functions, molecular size distribution, and dynamics of oligosaccharide mixtures.
32045528	4	50	theme	unique	872:877	arg1	way					879:881	a unique way	870:881	a unique way to elucidate the composition of mixtures	870:922	Three dimensional correlation plots displaying 13C diffusivity (DOSY: Diffusion Ordered SpectroscopY), relaxation parameters (TOSY: raTe of relaxation Ordered SpectrscopY), and chemical shift offer a unique way to elucidate the composition of mixtures.
32045528	5	51	theme	multifaceted	951:962	arg1	strategy					987:994	this multifaceted 13C probed correlation strategy	946:994	this multifaceted 13C probed correlation strategy in standard mixtures of aliphatic and aromatic compounds	946:1051	We have demonstrated this multifaceted 13C probed correlation strategy in standard mixtures of aliphatic and aromatic compounds, before implementing it on AXOS.
32045528	1	52	theme	fibers	301:306	arg1	arabinoxylan					268:279	arabinoxylan	268:279	arabinoxylan	268:279	Arabinoxylan oligosaccharides (AXOS) are a complex mixture of cereal derived, water-soluble prebiotics, obtained by enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects.
32045528	1	52	theme	fibers	301:306	arg1	group					284:288	a group	282:288	a group of dietary fibers exerting numerous nutritional and health-beneficial effects	282:366	Arabinoxylan oligosaccharides (AXOS) are a complex mixture of cereal derived, water-soluble prebiotics, obtained by enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects.
32045528	1	52	theme	fibers	301:306	arg1	fibers					301:306	dietary fibers	293:306	dietary fibers exerting numerous nutritional and health-beneficial effects	293:366	Arabinoxylan oligosaccharides (AXOS) are a complex mixture of cereal derived, water-soluble prebiotics, obtained by enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects.
32045528	3	53	dep	in	506:507	arg1	situ					509:512	situ	509:512	situ	509:512	Here we present the in situ analysis of AXOS using a variety of state-of-the-art sensitivity-enhanced 13C-DOSY methods, enabling virtual separation and identification of the components.
32045528	0	54	dep	In	34:35	arg1	Situ					37:40	Situ	37:40	Situ	37:40	13C-DOSY-TOSY NMR Correlation for In Situ Analysis of Structure, Size Distribution, and Dynamics of Prebiotic Oligosaccharides.
32045528	4	55	dep	TOSY	798:801	arg1	raTe					804:807	raTe	804:807	TOSY: raTe of relaxation Ordered SpectrscopY	798:841	Three dimensional correlation plots displaying 13C diffusivity (DOSY: Diffusion Ordered SpectroscopY), relaxation parameters (TOSY: raTe of relaxation Ordered SpectrscopY), and chemical shift offer a unique way to elucidate the composition of mixtures.
32045528	6	56	theme	oligosaccharide	1269:1283	arg1	mixtures					1285:1292	oligosaccharide mixtures	1269:1292	oligosaccharide mixtures	1269:1292	These 3D-DOSY-TOSY plots in combination with 2D-NMR correlation experiments offer unprecedented clarity for assigning chemical functions, molecular size distribution, and dynamics of oligosaccharide mixtures.
32045528	4	57	dep	DOSY	736:739	arg1	SpectroscopY					760:771	Diffusion Ordered SpectroscopY	742:771	DOSY: Diffusion Ordered SpectroscopY	736:771	Three dimensional correlation plots displaying 13C diffusivity (DOSY: Diffusion Ordered SpectroscopY), relaxation parameters (TOSY: raTe of relaxation Ordered SpectrscopY), and chemical shift offer a unique way to elucidate the composition of mixtures.
32045528	1	58	dep	derived	197:203	arg1	water-soluble					206:218	water-soluble	206:218	water-soluble	206:218	Arabinoxylan oligosaccharides (AXOS) are a complex mixture of cereal derived, water-soluble prebiotics, obtained by enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects.
32045528	3	59	theme	in	506:507	arg1	analysis					514:521	the in situ analysis	502:521	the in situ analysis of AXOS using a variety of state-of-the-art sensitivity-enhanced 13C-DOSY methods	502:603	Here we present the in situ analysis of AXOS using a variety of state-of-the-art sensitivity-enhanced 13C-DOSY methods, enabling virtual separation and identification of the components.
32045528	5	60	theme	standard	999:1006	arg1	mixtures					1008:1015	standard mixtures	999:1015	standard mixtures of aliphatic and aromatic compounds	999:1051	We have demonstrated this multifaceted 13C probed correlation strategy in standard mixtures of aliphatic and aromatic compounds, before implementing it on AXOS.
32045528	4	61	theme	Ordered	823:829	arg1	SpectrscopY					831:841	relaxation Ordered SpectrscopY	812:841	relaxation Ordered SpectrscopY	812:841	Three dimensional correlation plots displaying 13C diffusivity (DOSY: Diffusion Ordered SpectroscopY), relaxation parameters (TOSY: raTe of relaxation Ordered SpectrscopY), and chemical shift offer a unique way to elucidate the composition of mixtures.
32045528	0	62	dep	Correlation	18:28	arg1	Analysis					42:49	In Situ Analysis	34:49	In Situ Analysis of Structure, Size Distribution, and Dynamics of Prebiotic Oligosaccharides	34:125	13C-DOSY-TOSY NMR Correlation for In Situ Analysis of Structure, Size Distribution, and Dynamics of Prebiotic Oligosaccharides.
32045528	1	63	theme	Arabinoxylan	128:139	arg1	mixture					179:185	a complex mixture	169:185	a complex mixture	169:185	Arabinoxylan oligosaccharides (AXOS) are a complex mixture of cereal derived, water-soluble prebiotics, obtained by enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects.
32045528	1	63	theme	Arabinoxylan	128:139	arg1	AXOS					159:162	AXOS	159:162	AXOS	159:162	Arabinoxylan oligosaccharides (AXOS) are a complex mixture of cereal derived, water-soluble prebiotics, obtained by enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects.
32045528	1	63	theme	Arabinoxylan	128:139	arg1	oligosaccharides					141:156	Arabinoxylan oligosaccharides	128:156	Arabinoxylan oligosaccharides (AXOS)	128:163	Arabinoxylan oligosaccharides (AXOS) are a complex mixture of cereal derived, water-soluble prebiotics, obtained by enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects.
32045528	5	64	from	strategy	987:994	arg1	mixtures					1008:1015	standard mixtures	999:1015	standard mixtures of aliphatic and aromatic compounds	999:1051	We have demonstrated this multifaceted 13C probed correlation strategy in standard mixtures of aliphatic and aromatic compounds, before implementing it on AXOS.
32045528	1	65	theme	prebiotics	220:229	arg1	mixture					179:185	a complex mixture	169:185	a complex mixture	169:185	Arabinoxylan oligosaccharides (AXOS) are a complex mixture of cereal derived, water-soluble prebiotics, obtained by enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects.
32045528	1	65	theme	prebiotics	220:229	arg1	oligosaccharides					141:156	Arabinoxylan oligosaccharides	128:156	Arabinoxylan oligosaccharides (AXOS)	128:163	Arabinoxylan oligosaccharides (AXOS) are a complex mixture of cereal derived, water-soluble prebiotics, obtained by enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects.
32045528	6	66	theme	unprecedented	1168:1180	arg1	clarity					1182:1188	unprecedented clarity	1168:1188	unprecedented clarity for assigning chemical functions, molecular size distribution, and dynamics of oligosaccharide mixtures	1168:1292	These 3D-DOSY-TOSY plots in combination with 2D-NMR correlation experiments offer unprecedented clarity for assigning chemical functions, molecular size distribution, and dynamics of oligosaccharide mixtures.
32045528	4	67	theme	chemical	849:856	arg1	shift					858:862	chemical shift	849:862	chemical shift	849:862	Three dimensional correlation plots displaying 13C diffusivity (DOSY: Diffusion Ordered SpectroscopY), relaxation parameters (TOSY: raTe of relaxation Ordered SpectrscopY), and chemical shift offer a unique way to elucidate the composition of mixtures.
32045528	2	68	theme	complex	374:380	arg1	mixtures					395:402	Such complex biomolecular mixtures	369:402	Such complex biomolecular mixtures	369:402	Such complex biomolecular mixtures are notoriously difficult to characterize without initial physical fractionation.
32045528	6	69	theme	2D-NMR	1131:1136	arg1	experiments					1150:1160	2D-NMR correlation experiments	1131:1160	2D-NMR correlation experiments	1131:1160	These 3D-DOSY-TOSY plots in combination with 2D-NMR correlation experiments offer unprecedented clarity for assigning chemical functions, molecular size distribution, and dynamics of oligosaccharide mixtures.
32045528	0	70	theme	Structure	54:62	arg1	Analysis					42:49	In Situ Analysis	34:49	In Situ Analysis of Structure, Size Distribution, and Dynamics of Prebiotic Oligosaccharides	34:125	13C-DOSY-TOSY NMR Correlation for In Situ Analysis of Structure, Size Distribution, and Dynamics of Prebiotic Oligosaccharides.
32045528	1	71	theme	numerous	317:324	arg1	effects					360:366	numerous nutritional and health-beneficial effects	317:366	numerous nutritional and health-beneficial effects	317:366	Arabinoxylan oligosaccharides (AXOS) are a complex mixture of cereal derived, water-soluble prebiotics, obtained by enzymatic hydrolysis of arabinoxylan, a group of dietary fibers exerting numerous nutritional and health-beneficial effects.
32045528	3	72	theme	components	660:669	arg1	identification					638:651	identification	638:651	identification	638:651	Here we present the in situ analysis of AXOS using a variety of state-of-the-art sensitivity-enhanced 13C-DOSY methods, enabling virtual separation and identification of the components.
32045528	3	72	theme	components	660:669	arg1	separation					623:632	virtual separation	615:632	virtual separation	615:632	Here we present the in situ analysis of AXOS using a variety of state-of-the-art sensitivity-enhanced 13C-DOSY methods, enabling virtual separation and identification of the components.
32045528	2	73	theme	Such	369:372	arg1	mixtures					395:402	Such complex biomolecular mixtures	369:402	Such complex biomolecular mixtures	369:402	Such complex biomolecular mixtures are notoriously difficult to characterize without initial physical fractionation.
32813756	2	0	theme	Metagenomic	479:489	arg1	analysis					491:498	Metagenomic analysis	479:498	Metagenomic analysis	479:498	Metagenomic analysis revealed that microbial communities in the two types of inoculum were distinctively different, which led to distinct fermentation characteristics with the polysaccharides.
32813756	4	1	theme	operational	1018:1028	arg1	copri					959:963	Prevotella copri	948:963	Prevotella copri	948:963	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	4	1	theme	operational	1018:1028	arg1	rc4-4					994:998	the unclassified lineage rc4-4	969:998	the unclassified lineage rc4-4	969:998	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	4	1	theme	operational	1018:1028	arg1	OTUs					1047:1050	OTUs	1047:1050	OTUs	1047:1050	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	4	1	theme	operational	1018:1028	arg1	units					1040:1044	the dominant operational taxonomic units	1005:1044	the dominant operational taxonomic units (OTUs) promoted during fermentation	1005:1080	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	4	2	theme	fecal	900:904	arg1	inoculum					906:913	the porcine fecal inoculum	888:913	the porcine fecal inoculum	888:913	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	8	3	theme	fatty	1537:1541	arg1	SCFA					1549:1552	SCFA	1549:1552	SCFA	1549:1552	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	8	3	theme	fatty	1537:1541	arg1	acid					1543:1546	the short chain fatty acid	1521:1546	the short chain fatty acid (SCFA) production	1521:1564	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	6	4	dep	uniformis	1425:1433	arg1	an					1354:1355	an	1354:1355	an	1354:1355	Specifically, WAX promoted the growth of Bacteroides plebeius and a Blautia sp., while GXG promoted an unclassified Bacteroidales, Parabacteroides distasonis, Bacteroides uniformis and Bacteroides sp.
32813756	6	4	dep	uniformis	1425:1433	arg1	sp					1451:1452	sp	1451:1452	sp	1451:1452	Specifically, WAX promoted the growth of Bacteroides plebeius and a Blautia sp., while GXG promoted an unclassified Bacteroidales, Parabacteroides distasonis, Bacteroides uniformis and Bacteroides sp.
32813756	0	5	theme	fecal	79:83	arg1	inoculum					85:92	fecal inoculum	79:92	fecal inoculum more than substrate chemistry	79:122	In vitro fermentation outcomes of arabinoxylan and galactoxyloglucan depend on fecal inoculum more than substrate chemistry.
32813756	1	6	theme	unrestricted	333:344	arg1	diets					346:350	unrestricted diets	333:350	unrestricted diets	333:350	Using in vitro fermentation conditions, this study investigated the fermentation characteristics of arabinoxylan (AX) and xyloglucan (XG) with a fecal inoculum that was collected either from humans consuming unrestricted diets or pigs fed a semi-defined diet with cellulose being the sole non-starch polysaccharide for 10 days prior to fecal collection.
32813756	3	7	theme	microbial	676:684	arg1	communities					686:696	The microbial communities	672:696	The microbial communities fermented with the porcine fecal inoculum	672:738	The microbial communities fermented with the porcine fecal inoculum were clustered according to the fermentation time, while those fermented with the human fecal inoculum were differentiated by the substrates.
32813756	5	8	theme	wheat	1099:1103	arg1	WAX					1109:1111	wheat AX (WAX)	1099:1112	wheat AX (WAX)	1099:1112	Fermentation of wheat AX (WAX) and galacto-XG (GXG) with the human fecal inoculum, however, promoted different OTUs, except for a shared OTU belonging to Lachnospiraceae.
32813756	5	9	theme	AX	1105:1106	arg1	WAX					1109:1111	wheat AX (WAX)	1099:1112	wheat AX (WAX)	1099:1112	Fermentation of wheat AX (WAX) and galacto-XG (GXG) with the human fecal inoculum, however, promoted different OTUs, except for a shared OTU belonging to Lachnospiraceae.
32813756	4	10	theme	unclassified	973:984	arg1	rc4-4					994:998	the unclassified lineage rc4-4	969:998	the unclassified lineage rc4-4	969:998	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	4	10	theme	unclassified	973:984	arg1	copri					959:963	Prevotella copri	948:963	Prevotella copri	948:963	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	4	10	theme	unclassified	973:984	arg1	units					1040:1044	the dominant operational taxonomic units	1005:1044	the dominant operational taxonomic units (OTUs) promoted during fermentation	1005:1080	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	3	11	theme	porcine	717:723	arg1	inoculum					731:738	the porcine fecal inoculum	713:738	the porcine fecal inoculum	713:738	The microbial communities fermented with the porcine fecal inoculum were clustered according to the fermentation time, while those fermented with the human fecal inoculum were differentiated by the substrates.
32813756	4	12	dep	units	1040:1044	arg1	Using					882:886	Using	882:886	Using the porcine fecal inoculum	882:913	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	1	13	dep	in	131:132	arg1	vitro					134:138	vitro	134:138	vitro	134:138	Using in vitro fermentation conditions, this study investigated the fermentation characteristics of arabinoxylan (AX) and xyloglucan (XG) with a fecal inoculum that was collected either from humans consuming unrestricted diets or pigs fed a semi-defined diet with cellulose being the sole non-starch polysaccharide for 10 days prior to fecal collection.
32813756	8	14	theme	SCFA	1691:1694	arg1	amounts					1664:1670	different amounts	1654:1670	different amounts	1654:1670	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	8	14	theme	SCFA	1691:1694	arg1	SCFA					1691:1694	SCFA	1691:1694	SCFA	1691:1694	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	8	14	theme	SCFA	1691:1694	arg1	proportions					1676:1686	proportions	1676:1686	proportions of SCFA	1676:1694	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	0	15	theme	more	94:97	arg1	inoculum					85:92	fecal inoculum	79:92	fecal inoculum more than substrate chemistry	79:122	In vitro fermentation outcomes of arabinoxylan and galactoxyloglucan depend on fecal inoculum more than substrate chemistry.
32813756	8	16	theme	chain	1531:1535	arg1	SCFA					1549:1552	SCFA	1549:1552	SCFA	1549:1552	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	8	16	theme	chain	1531:1535	arg1	acid					1543:1546	the short chain fatty acid	1521:1546	the short chain fatty acid (SCFA) production	1521:1564	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	9	17	theme	fermentation	1908:1919	arg1	outcomes					1921:1928	fermentation outcomes	1908:1928	fermentation outcomes	1908:1928	Altogether, this study indicated that the starting inoculum composition had a greater effect than polysaccharide chemistry in driving fermentation outcomes.
32813756	8	18	theme	fecal	1629:1633	arg1	fermentation					1635:1646	the porcine fecal fermentation	1617:1646	the porcine fecal fermentation	1617:1646	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	2	19	theme	microbial	514:522	arg1	communities					524:534	microbial communities	514:534	microbial communities in the two types of inoculum	514:563	Metagenomic analysis revealed that microbial communities in the two types of inoculum were distinctively different, which led to distinct fermentation characteristics with the polysaccharides.
32813756	5	20	theme	galacto-XG	1118:1127	arg1	Fermentation					1083:1094	Fermentation	1083:1094	Fermentation of wheat AX (WAX) and galacto-XG (GXG) with the human fecal inoculum	1083:1163	Fermentation of wheat AX (WAX) and galacto-XG (GXG) with the human fecal inoculum, however, promoted different OTUs, except for a shared OTU belonging to Lachnospiraceae.
32813756	8	21	with	fermentation	1715:1726	arg1	inoculum					1764:1771	the human fecal inoculum	1748:1771	the human fecal inoculum	1748:1771	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	0	22	theme	substrate	104:112	arg1	chemistry					114:122	substrate chemistry	104:122	substrate chemistry	104:122	In vitro fermentation outcomes of arabinoxylan and galactoxyloglucan depend on fecal inoculum more than substrate chemistry.
32813756	8	23	theme	acid	1543:1546	arg1	production					1555:1564	the short chain fatty acid (SCFA) production	1521:1564	the short chain fatty acid (SCFA) production	1521:1564	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	6	24	dep	Blautia	1322:1328	arg1	sp.					1330:1332	a Blautia sp.	1320:1332	a Blautia sp.	1320:1332	Specifically, WAX promoted the growth of Bacteroides plebeius and a Blautia sp., while GXG promoted an unclassified Bacteroidales, Parabacteroides distasonis, Bacteroides uniformis and Bacteroides sp.
32813756	1	25	theme	prior	452:456	arg1	days					447:450	10 days	444:450	10 days prior to fecal collection	444:476	Using in vitro fermentation conditions, this study investigated the fermentation characteristics of arabinoxylan (AX) and xyloglucan (XG) with a fecal inoculum that was collected either from humans consuming unrestricted diets or pigs fed a semi-defined diet with cellulose being the sole non-starch polysaccharide for 10 days prior to fecal collection.
32813756	4	26	theme	dominant	1009:1016	arg1	copri					959:963	Prevotella copri	948:963	Prevotella copri	948:963	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	4	26	theme	dominant	1009:1016	arg1	rc4-4					994:998	the unclassified lineage rc4-4	969:998	the unclassified lineage rc4-4	969:998	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	4	26	theme	dominant	1009:1016	arg1	OTUs					1047:1050	OTUs	1047:1050	OTUs	1047:1050	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	4	26	theme	dominant	1009:1016	arg1	units					1040:1044	the dominant operational taxonomic units	1005:1044	the dominant operational taxonomic units (OTUs) promoted during fermentation	1005:1080	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	2	27	theme	fermentation	617:628	arg1	characteristics					630:644	distinct fermentation characteristics	608:644	distinct fermentation characteristics with the polysaccharides	608:669	Metagenomic analysis revealed that microbial communities in the two types of inoculum were distinctively different, which led to distinct fermentation characteristics with the polysaccharides.
32813756	2	28	from	communities	524:534	arg1	types					547:551	the two types	539:551	the two types of inoculum	539:563	Metagenomic analysis revealed that microbial communities in the two types of inoculum were distinctively different, which led to distinct fermentation characteristics with the polysaccharides.
32813756	5	29	with	Fermentation	1083:1094	arg1	inoculum					1156:1163	the human fecal inoculum	1140:1163	the human fecal inoculum	1140:1163	Fermentation of wheat AX (WAX) and galacto-XG (GXG) with the human fecal inoculum, however, promoted different OTUs, except for a shared OTU belonging to Lachnospiraceae.
32813756	0	30	theme	In	0:1	arg1	outcomes					22:29	In vitro fermentation outcomes	0:29	In vitro fermentation outcomes of arabinoxylan and galactoxyloglucan	0:67	In vitro fermentation outcomes of arabinoxylan and galactoxyloglucan depend on fecal inoculum more than substrate chemistry.
32813756	2	31	theme	distinct	608:615	arg1	characteristics					630:644	distinct fermentation characteristics	608:644	distinct fermentation characteristics with the polysaccharides	608:669	Metagenomic analysis revealed that microbial communities in the two types of inoculum were distinctively different, which led to distinct fermentation characteristics with the polysaccharides.
32813756	1	32	theme	fecal	461:465	arg1	collection					467:476	fecal collection	461:476	fecal collection	461:476	Using in vitro fermentation conditions, this study investigated the fermentation characteristics of arabinoxylan (AX) and xyloglucan (XG) with a fecal inoculum that was collected either from humans consuming unrestricted diets or pigs fed a semi-defined diet with cellulose being the sole non-starch polysaccharide for 10 days prior to fecal collection.
32813756	1	33	theme	fermentation	193:204	arg1	characteristics					206:220	the fermentation characteristics	189:220	the fermentation characteristics of arabinoxylan (AX) and xyloglucan (XG)	189:261	Using in vitro fermentation conditions, this study investigated the fermentation characteristics of arabinoxylan (AX) and xyloglucan (XG) with a fecal inoculum that was collected either from humans consuming unrestricted diets or pigs fed a semi-defined diet with cellulose being the sole non-starch polysaccharide for 10 days prior to fecal collection.
32813756	1	34	theme	fecal	270:274	arg1	inoculum					276:283	a fecal inoculum	268:283	a fecal inoculum that was collected either from humans consuming unrestricted diets or pigs fed a semi-defined diet with cellulose being the sole non-starch polysaccharide for 10 days prior to fecal collection	268:476	Using in vitro fermentation conditions, this study investigated the fermentation characteristics of arabinoxylan (AX) and xyloglucan (XG) with a fecal inoculum that was collected either from humans consuming unrestricted diets or pigs fed a semi-defined diet with cellulose being the sole non-starch polysaccharide for 10 days prior to fecal collection.
32813756	4	35	theme	Prevotella	948:957	arg1	rc4-4					994:998	the unclassified lineage rc4-4	969:998	the unclassified lineage rc4-4	969:998	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	4	35	theme	Prevotella	948:957	arg1	copri					959:963	Prevotella copri	948:963	Prevotella copri	948:963	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	4	35	theme	Prevotella	948:957	arg1	units					1040:1044	the dominant operational taxonomic units	1005:1044	the dominant operational taxonomic units (OTUs) promoted during fermentation	1005:1080	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	4	36	theme	porcine	892:898	arg1	inoculum					906:913	the porcine fecal inoculum	888:913	the porcine fecal inoculum	888:913	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	8	37	theme	WAX	1731:1733	arg1	fermentation					1715:1726	fermentation	1715:1726	fermentation of WAX and GXG with the human fecal inoculum	1715:1771	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	0	38	theme	fermentation	9:20	arg1	outcomes					22:29	In vitro fermentation outcomes	0:29	In vitro fermentation outcomes of arabinoxylan and galactoxyloglucan	0:67	In vitro fermentation outcomes of arabinoxylan and galactoxyloglucan depend on fecal inoculum more than substrate chemistry.
32813756	1	39	dep	humans	316:321	arg1	either					304:309	either	304:309	either	304:309	Using in vitro fermentation conditions, this study investigated the fermentation characteristics of arabinoxylan (AX) and xyloglucan (XG) with a fecal inoculum that was collected either from humans consuming unrestricted diets or pigs fed a semi-defined diet with cellulose being the sole non-starch polysaccharide for 10 days prior to fecal collection.
32813756	1	40	theme	semi-defined	366:377	arg1	diet					379:382	a semi-defined diet	364:382	a semi-defined diet	364:382	Using in vitro fermentation conditions, this study investigated the fermentation characteristics of arabinoxylan (AX) and xyloglucan (XG) with a fecal inoculum that was collected either from humans consuming unrestricted diets or pigs fed a semi-defined diet with cellulose being the sole non-starch polysaccharide for 10 days prior to fecal collection.
32813756	8	41	theme	GXG	1739:1741	arg1	fermentation					1715:1726	fermentation	1715:1726	fermentation of WAX and GXG with the human fecal inoculum	1715:1771	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	0	42	theme	arabinoxylan	34:45	arg1	outcomes					22:29	In vitro fermentation outcomes	0:29	In vitro fermentation outcomes of arabinoxylan and galactoxyloglucan	0:67	In vitro fermentation outcomes of arabinoxylan and galactoxyloglucan depend on fecal inoculum more than substrate chemistry.
32813756	3	43	theme	fermentation	772:783	arg1	time					785:788	the fermentation time	768:788	the fermentation time	768:788	The microbial communities fermented with the porcine fecal inoculum were clustered according to the fermentation time, while those fermented with the human fecal inoculum were differentiated by the substrates.
32813756	6	44	theme	Blautia	1322:1328	arg1	growth					1285:1290	the growth	1281:1290	the growth of Bacteroides plebeius and a Blautia sp.	1281:1332	Specifically, WAX promoted the growth of Bacteroides plebeius and a Blautia sp., while GXG promoted an unclassified Bacteroidales, Parabacteroides distasonis, Bacteroides uniformis and Bacteroides sp.
32813756	5	45	theme	shared	1213:1218	arg1	OTU					1220:1222	a shared OTU	1211:1222	a shared OTU belonging to Lachnospiraceae	1211:1251	Fermentation of wheat AX (WAX) and galacto-XG (GXG) with the human fecal inoculum, however, promoted different OTUs, except for a shared OTU belonging to Lachnospiraceae.
32813756	8	46	theme	porcine	1621:1627	arg1	fermentation					1635:1646	the porcine fecal fermentation	1617:1646	the porcine fecal fermentation	1617:1646	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	2	47	with	characteristics	630:644	arg1	polysaccharides					655:669	the polysaccharides	651:669	the polysaccharides	651:669	Metagenomic analysis revealed that microbial communities in the two types of inoculum were distinctively different, which led to distinct fermentation characteristics with the polysaccharides.
32813756	1	48	theme	arabinoxylan	225:236	arg1	characteristics					206:220	the fermentation characteristics	189:220	the fermentation characteristics of arabinoxylan (AX) and xyloglucan (XG)	189:261	Using in vitro fermentation conditions, this study investigated the fermentation characteristics of arabinoxylan (AX) and xyloglucan (XG) with a fecal inoculum that was collected either from humans consuming unrestricted diets or pigs fed a semi-defined diet with cellulose being the sole non-starch polysaccharide for 10 days prior to fecal collection.
32813756	4	49	theme	taxonomic	1030:1038	arg1	copri					959:963	Prevotella copri	948:963	Prevotella copri	948:963	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	4	49	theme	taxonomic	1030:1038	arg1	rc4-4					994:998	the unclassified lineage rc4-4	969:998	the unclassified lineage rc4-4	969:998	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	4	49	theme	taxonomic	1030:1038	arg1	OTUs					1047:1050	OTUs	1047:1050	OTUs	1047:1050	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	4	49	theme	taxonomic	1030:1038	arg1	units					1040:1044	the dominant operational taxonomic units	1005:1044	the dominant operational taxonomic units (OTUs) promoted during fermentation	1005:1080	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	5	50	theme	fecal	1150:1154	arg1	inoculum					1156:1163	the human fecal inoculum	1140:1163	the human fecal inoculum	1140:1163	Fermentation of wheat AX (WAX) and galacto-XG (GXG) with the human fecal inoculum, however, promoted different OTUs, except for a shared OTU belonging to Lachnospiraceae.
32813756	9	51	theme	inoculum	1825:1832	arg1	composition					1834:1844	the starting inoculum composition	1812:1844	the starting inoculum composition	1812:1844	Altogether, this study indicated that the starting inoculum composition had a greater effect than polysaccharide chemistry in driving fermentation outcomes.
32813756	5	52	theme	WAX	1109:1111	arg1	Fermentation					1083:1094	Fermentation	1083:1094	Fermentation of wheat AX (WAX) and galacto-XG (GXG) with the human fecal inoculum	1083:1163	Fermentation of wheat AX (WAX) and galacto-XG (GXG) with the human fecal inoculum, however, promoted different OTUs, except for a shared OTU belonging to Lachnospiraceae.
32813756	8	53	theme	human	1752:1756	arg1	inoculum					1764:1771	the human fecal inoculum	1748:1771	the human fecal inoculum	1748:1771	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	3	54	theme	human	822:826	arg1	inoculum					834:841	the human fecal inoculum	818:841	the human fecal inoculum	818:841	The microbial communities fermented with the porcine fecal inoculum were clustered according to the fermentation time, while those fermented with the human fecal inoculum were differentiated by the substrates.
32813756	0	55	theme	galactoxyloglucan	51:67	arg1	outcomes					22:29	In vitro fermentation outcomes	0:29	In vitro fermentation outcomes of arabinoxylan and galactoxyloglucan	0:67	In vitro fermentation outcomes of arabinoxylan and galactoxyloglucan depend on fecal inoculum more than substrate chemistry.
32813756	5	56	theme	human	1144:1148	arg1	inoculum					1156:1163	the human fecal inoculum	1140:1163	the human fecal inoculum	1140:1163	Fermentation of wheat AX (WAX) and galacto-XG (GXG) with the human fecal inoculum, however, promoted different OTUs, except for a shared OTU belonging to Lachnospiraceae.
32813756	8	57	theme	SCFA	1584:1587	arg1	profiles					1589:1596	comparable SCFA profiles	1573:1596	comparable SCFA profiles	1573:1596	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	6	58	theme	plebeius	1307:1314	arg1	growth					1285:1290	the growth	1281:1290	the growth of Bacteroides plebeius and a Blautia sp.	1281:1332	Specifically, WAX promoted the growth of Bacteroides plebeius and a Blautia sp., while GXG promoted an unclassified Bacteroidales, Parabacteroides distasonis, Bacteroides uniformis and Bacteroides sp.
32813756	8	59	theme	bacterial	1475:1483	arg1	communities					1485:1495	bacterial communities	1475:1495	bacterial communities	1475:1495	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	8	60	theme	different	1654:1662	arg1	amounts					1664:1670	different amounts	1654:1670	different amounts	1654:1670	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	8	60	theme	different	1654:1662	arg1	SCFA					1691:1694	SCFA	1691:1694	SCFA	1691:1694	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	8	61	theme	comparable	1573:1582	arg1	profiles					1589:1596	comparable SCFA profiles	1573:1596	comparable SCFA profiles	1573:1596	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	9	62	contain	had	1846:1848	arg1	composition					1834:1844	the starting inoculum composition	1812:1844	the starting inoculum composition	1812:1844	Altogether, this study indicated that the starting inoculum composition had a greater effect than polysaccharide chemistry in driving fermentation outcomes.
32813756	9	62	contain	had	1846:1848	arg2	effect					1860:1865	a greater effect	1850:1865	a greater effect	1850:1865	Altogether, this study indicated that the starting inoculum composition had a greater effect than polysaccharide chemistry in driving fermentation outcomes.
32813756	1	63	dep	arabinoxylan	225:236	arg1	AX					239:240	AX	239:240	AX	239:240	Using in vitro fermentation conditions, this study investigated the fermentation characteristics of arabinoxylan (AX) and xyloglucan (XG) with a fecal inoculum that was collected either from humans consuming unrestricted diets or pigs fed a semi-defined diet with cellulose being the sole non-starch polysaccharide for 10 days prior to fecal collection.
32813756	8	64	theme	fecal	1758:1762	arg1	inoculum					1764:1771	the human fecal inoculum	1748:1771	the human fecal inoculum	1748:1771	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	1	65	theme	in	131:132	arg1	conditions					153:162	in vitro fermentation conditions	131:162	in vitro fermentation conditions	131:162	Using in vitro fermentation conditions, this study investigated the fermentation characteristics of arabinoxylan (AX) and xyloglucan (XG) with a fecal inoculum that was collected either from humans consuming unrestricted diets or pigs fed a semi-defined diet with cellulose being the sole non-starch polysaccharide for 10 days prior to fecal collection.
32813756	9	66	theme	greater	1852:1858	arg1	effect					1860:1865	a greater effect	1850:1865	a greater effect	1850:1865	Altogether, this study indicated that the starting inoculum composition had a greater effect than polysaccharide chemistry in driving fermentation outcomes.
32813756	3	67	theme	fecal	828:832	arg1	inoculum					834:841	the human fecal inoculum	818:841	the human fecal inoculum	818:841	The microbial communities fermented with the porcine fecal inoculum were clustered according to the fermentation time, while those fermented with the human fecal inoculum were differentiated by the substrates.
32813756	2	68	theme	inoculum	556:563	arg1	types					547:551	the two types	539:551	the two types of inoculum	539:563	Metagenomic analysis revealed that microbial communities in the two types of inoculum were distinctively different, which led to distinct fermentation characteristics with the polysaccharides.
32813756	1	69	theme	sole	409:412	arg1	polysaccharide					425:438	the sole non-starch polysaccharide	405:438	the sole non-starch polysaccharide for 10 days prior to fecal collection	405:476	Using in vitro fermentation conditions, this study investigated the fermentation characteristics of arabinoxylan (AX) and xyloglucan (XG) with a fecal inoculum that was collected either from humans consuming unrestricted diets or pigs fed a semi-defined diet with cellulose being the sole non-starch polysaccharide for 10 days prior to fecal collection.
32813756	1	69	theme	sole	409:412	arg1	cellulose					389:397	cellulose	389:397	cellulose	389:397	Using in vitro fermentation conditions, this study investigated the fermentation characteristics of arabinoxylan (AX) and xyloglucan (XG) with a fecal inoculum that was collected either from humans consuming unrestricted diets or pigs fed a semi-defined diet with cellulose being the sole non-starch polysaccharide for 10 days prior to fecal collection.
32813756	8	70	theme	short	1525:1529	arg1	SCFA					1549:1552	SCFA	1549:1552	SCFA	1549:1552	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	8	70	theme	short	1525:1529	arg1	acid					1543:1546	the short chain fatty acid	1521:1546	the short chain fatty acid (SCFA) production	1521:1564	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	9	71	theme	starting	1816:1823	arg1	inoculum					1825:1832	the starting inoculum	1812:1832	the starting inoculum composition	1812:1844	Altogether, this study indicated that the starting inoculum composition had a greater effect than polysaccharide chemistry in driving fermentation outcomes.
32813756	8	72	from	changes	1464:1470	arg1	communities					1485:1495	bacterial communities	1475:1495	bacterial communities	1475:1495	These changes in bacterial communities were in accordance with the short chain fatty acid (SCFA) production, where comparable SCFA profiles were obtained from the porcine fecal fermentation while different amounts and proportions of SCFA were acquired from fermentation of WAX and GXG with the human fecal inoculum.
32813756	1	73	theme	non-starch	414:423	arg1	polysaccharide					425:438	the sole non-starch polysaccharide	405:438	the sole non-starch polysaccharide for 10 days prior to fecal collection	405:476	Using in vitro fermentation conditions, this study investigated the fermentation characteristics of arabinoxylan (AX) and xyloglucan (XG) with a fecal inoculum that was collected either from humans consuming unrestricted diets or pigs fed a semi-defined diet with cellulose being the sole non-starch polysaccharide for 10 days prior to fecal collection.
32813756	1	73	theme	non-starch	414:423	arg1	cellulose					389:397	cellulose	389:397	cellulose	389:397	Using in vitro fermentation conditions, this study investigated the fermentation characteristics of arabinoxylan (AX) and xyloglucan (XG) with a fecal inoculum that was collected either from humans consuming unrestricted diets or pigs fed a semi-defined diet with cellulose being the sole non-starch polysaccharide for 10 days prior to fecal collection.
32813756	1	74	theme	fermentation	140:151	arg1	conditions					153:162	in vitro fermentation conditions	131:162	in vitro fermentation conditions	131:162	Using in vitro fermentation conditions, this study investigated the fermentation characteristics of arabinoxylan (AX) and xyloglucan (XG) with a fecal inoculum that was collected either from humans consuming unrestricted diets or pigs fed a semi-defined diet with cellulose being the sole non-starch polysaccharide for 10 days prior to fecal collection.
32813756	3	75	theme	fecal	725:729	arg1	inoculum					731:738	the porcine fecal inoculum	713:738	the porcine fecal inoculum	713:738	The microbial communities fermented with the porcine fecal inoculum were clustered according to the fermentation time, while those fermented with the human fecal inoculum were differentiated by the substrates.
32813756	0	76	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro fermentation outcomes of arabinoxylan and galactoxyloglucan depend on fecal inoculum more than substrate chemistry.
32813756	5	77	theme	different	1184:1192	arg1	OTUs					1194:1197	different OTUs	1184:1197	different OTUs	1184:1197	Fermentation of wheat AX (WAX) and galacto-XG (GXG) with the human fecal inoculum, however, promoted different OTUs, except for a shared OTU belonging to Lachnospiraceae.
32813756	4	78	theme	lineage	986:992	arg1	rc4-4					994:998	the unclassified lineage rc4-4	969:998	the unclassified lineage rc4-4	969:998	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	4	78	theme	lineage	986:992	arg1	copri					959:963	Prevotella copri	948:963	Prevotella copri	948:963	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	4	78	theme	lineage	986:992	arg1	units					1040:1044	the dominant operational taxonomic units	1005:1044	the dominant operational taxonomic units (OTUs) promoted during fermentation	1005:1080	Using the porcine fecal inoculum, irrespective of the substrates, Prevotella copri and the unclassified lineage rc4-4 were the dominant operational taxonomic units (OTUs) promoted during fermentation.
32813756	9	79	theme	polysaccharide	1872:1885	arg1	chemistry					1887:1895	polysaccharide chemistry	1872:1895	polysaccharide chemistry	1872:1895	Altogether, this study indicated that the starting inoculum composition had a greater effect than polysaccharide chemistry in driving fermentation outcomes.
32813756	1	80	theme	xyloglucan	247:256	arg1	characteristics					206:220	the fermentation characteristics	189:220	the fermentation characteristics of arabinoxylan (AX) and xyloglucan (XG)	189:261	Using in vitro fermentation conditions, this study investigated the fermentation characteristics of arabinoxylan (AX) and xyloglucan (XG) with a fecal inoculum that was collected either from humans consuming unrestricted diets or pigs fed a semi-defined diet with cellulose being the sole non-starch polysaccharide for 10 days prior to fecal collection.
34103550	3	0	theme	mature	741:746	arg1	cell					771:774	mature differentiated phellem cell	741:774	mature differentiated phellem cell	741:774	Since cork oak (Quercus suber) phellogen is seasonally activated and its proliferation and specification to phellem cells is a continuous developmental process, the differentially expressed genes during the cork seasonal growth served us to identify molecular processes embracing from phellogen to mature differentiated phellem cell.
34103550	4	1	theme	cell	905:908	arg1	differentiation					910:924	cell differentiation	905:924	cell differentiation	905:924	At the beginning of cork growth (April), cell cycle regulation, meristem proliferation and maintenance and processes triggering cell differentiation were upregulated, showing an enrichment of phellogenic cells from which phellem cells are specified.
34103550	5	2	theme	cell	1131:1134	arg1	composition					1145:1155	the phellem cell chemical composition	1119:1155	the phellem cell chemical composition	1119:1155	Instead, at maximum (June) and advanced (July) cork growth, metabolic processes paralleling the phellem cell chemical composition, such as the biosynthesis of suberin, lignin, triterpenes and soluble aromatic compounds, were upregulated.
34103550	1	3	theme	cambium	164:170	arg1	cells					177:181	The phellogen or cork cambium stem cells	142:181	cells	177:181	The phellogen or cork cambium stem cells that divide periclinally and outwardly specify phellem or cork.
34103550	5	4	theme	triterpenes	1203:1213	arg1	biosynthesis					1170:1181	the biosynthesis	1166:1181	the biosynthesis of suberin, lignin, triterpenes and soluble aromatic compounds	1166:1244	Instead, at maximum (June) and advanced (July) cork growth, metabolic processes paralleling the phellem cell chemical composition, such as the biosynthesis of suberin, lignin, triterpenes and soluble aromatic compounds, were upregulated.
34103550	0	5	theme	developmental	81:93	arg1	processes					95:103	regulatory and developmental processes	66:103	regulatory and developmental processes	66:103	Transcriptomic analysis of cork during seasonal growth highlights regulatory and developmental processes from phellogen to phellem formation.
34103550	1	6	theme	stem	172:175	arg1	cells					177:181	The phellogen or cork cambium stem cells	142:181	cells	177:181	The phellogen or cork cambium stem cells that divide periclinally and outwardly specify phellem or cork.
34103550	6	7	attach	presented	1353:1361	arg1	July					1281:1284	July	1281:1284	July	1281:1284	Particularly in July, polysaccharides- and lignin-related secondary cell wall processes presented a maximal expression, indicating a cell wall reinforcement in the later stages of cork formation, presumably related with the initiation of latecork development.
34103550	6	7	attach	presented	1353:1361	arg2	processes					1343:1351	polysaccharides- and lignin-related secondary cell wall processes	1287:1351	polysaccharides- and lignin-related secondary cell wall processes	1287:1351	Particularly in July, polysaccharides- and lignin-related secondary cell wall processes presented a maximal expression, indicating a cell wall reinforcement in the later stages of cork formation, presumably related with the initiation of latecork development.
34103550	6	8	theme	lignin-related	1308:1321	arg1	processes					1343:1351	polysaccharides- and lignin-related secondary cell wall processes	1287:1351	polysaccharides- and lignin-related secondary cell wall processes	1287:1351	Particularly in July, polysaccharides- and lignin-related secondary cell wall processes presented a maximal expression, indicating a cell wall reinforcement in the later stages of cork formation, presumably related with the initiation of latecork development.
34103550	6	9	theme	cell	1398:1401	arg1	reinforcement					1408:1420	a cell wall reinforcement	1396:1420	a cell wall reinforcement	1396:1420	Particularly in July, polysaccharides- and lignin-related secondary cell wall processes presented a maximal expression, indicating a cell wall reinforcement in the later stages of cork formation, presumably related with the initiation of latecork development.
34103550	6	10	theme	polysaccharides-	1287:1302	arg1	processes					1343:1351	polysaccharides- and lignin-related secondary cell wall processes	1287:1351	polysaccharides- and lignin-related secondary cell wall processes	1287:1351	Particularly in July, polysaccharides- and lignin-related secondary cell wall processes presented a maximal expression, indicating a cell wall reinforcement in the later stages of cork formation, presumably related with the initiation of latecork development.
34103550	2	11	theme	radially-growing	305:320	arg1	organs					328:333	the radially-growing plant organs	301:333	the radially-growing plant organs	301:333	Despite the vital importance of phellem in protecting the radially-growing plant organs and wounded tissues, practically only the suberin biosynthetic process has been studied molecularly so far.
34103550	3	12	theme	developmental	581:593	arg1	specification					534:546	specification	534:546	specification	534:546	Since cork oak (Quercus suber) phellogen is seasonally activated and its proliferation and specification to phellem cells is a continuous developmental process, the differentially expressed genes during the cork seasonal growth served us to identify molecular processes embracing from phellogen to mature differentiated phellem cell.
34103550	3	12	theme	developmental	581:593	arg1	process					595:601	a continuous developmental process	568:601	a continuous developmental process	568:601	Since cork oak (Quercus suber) phellogen is seasonally activated and its proliferation and specification to phellem cells is a continuous developmental process, the differentially expressed genes during the cork seasonal growth served us to identify molecular processes embracing from phellogen to mature differentiated phellem cell.
34103550	3	12	theme	developmental	581:593	arg1	proliferation					516:528	its proliferation	512:528	its proliferation	512:528	Since cork oak (Quercus suber) phellogen is seasonally activated and its proliferation and specification to phellem cells is a continuous developmental process, the differentially expressed genes during the cork seasonal growth served us to identify molecular processes embracing from phellogen to mature differentiated phellem cell.
34103550	5	13	theme	soluble	1219:1225	arg1	compounds					1236:1244	soluble aromatic compounds	1219:1244	soluble aromatic compounds	1219:1244	Instead, at maximum (June) and advanced (July) cork growth, metabolic processes paralleling the phellem cell chemical composition, such as the biosynthesis of suberin, lignin, triterpenes and soluble aromatic compounds, were upregulated.
34103550	4	14	theme	cork	797:800	arg1	growth					802:807	cork growth	797:807	cork growth (April)	797:815	At the beginning of cork growth (April), cell cycle regulation, meristem proliferation and maintenance and processes triggering cell differentiation were upregulated, showing an enrichment of phellogenic cells from which phellem cells are specified.
34103550	4	14	theme	cork	797:800	arg1	April					810:814	April	810:814	April	810:814	At the beginning of cork growth (April), cell cycle regulation, meristem proliferation and maintenance and processes triggering cell differentiation were upregulated, showing an enrichment of phellogenic cells from which phellem cells are specified.
34103550	2	15	theme	suberin	377:383	arg1	process					398:404	practically only the suberin biosynthetic process	356:404	practically only the suberin biosynthetic process	356:404	Despite the vital importance of phellem in protecting the radially-growing plant organs and wounded tissues, practically only the suberin biosynthetic process has been studied molecularly so far.
34103550	5	16	theme	aromatic	1227:1234	arg1	compounds					1236:1244	soluble aromatic compounds	1219:1244	soluble aromatic compounds	1219:1244	Instead, at maximum (June) and advanced (July) cork growth, metabolic processes paralleling the phellem cell chemical composition, such as the biosynthesis of suberin, lignin, triterpenes and soluble aromatic compounds, were upregulated.
34103550	0	17	theme	phellem	123:129	arg1	formation					131:139	phellem formation	123:139	phellem formation	123:139	Transcriptomic analysis of cork during seasonal growth highlights regulatory and developmental processes from phellogen to phellem formation.
34103550	3	18	theme	phellem	551:557	arg1	cells					559:563	phellem cells	551:563	phellem cells	551:563	Since cork oak (Quercus suber) phellogen is seasonally activated and its proliferation and specification to phellem cells is a continuous developmental process, the differentially expressed genes during the cork seasonal growth served us to identify molecular processes embracing from phellogen to mature differentiated phellem cell.
34103550	4	19	theme	phellem	998:1004	arg1	cells					1006:1010	phellem cells	998:1010	phellem cells	998:1010	At the beginning of cork growth (April), cell cycle regulation, meristem proliferation and maintenance and processes triggering cell differentiation were upregulated, showing an enrichment of phellogenic cells from which phellem cells are specified.
34103550	3	20	theme	differentiated	748:761	arg1	cell					771:774	mature differentiated phellem cell	741:774	mature differentiated phellem cell	741:774	Since cork oak (Quercus suber) phellogen is seasonally activated and its proliferation and specification to phellem cells is a continuous developmental process, the differentially expressed genes during the cork seasonal growth served us to identify molecular processes embracing from phellogen to mature differentiated phellem cell.
34103550	6	21	theme	cork	1445:1448	arg1	formation					1450:1458	cork formation	1445:1458	cork formation	1445:1458	Particularly in July, polysaccharides- and lignin-related secondary cell wall processes presented a maximal expression, indicating a cell wall reinforcement in the later stages of cork formation, presumably related with the initiation of latecork development.
34103550	3	22	theme	Quercus	459:465	arg1	phellogen					474:482	cork oak (Quercus suber) phellogen	449:482	cork oak (Quercus suber) phellogen	449:482	Since cork oak (Quercus suber) phellogen is seasonally activated and its proliferation and specification to phellem cells is a continuous developmental process, the differentially expressed genes during the cork seasonal growth served us to identify molecular processes embracing from phellogen to mature differentiated phellem cell.
34103550	3	23	theme	phellem	763:769	arg1	cell					771:774	mature differentiated phellem cell	741:774	mature differentiated phellem cell	741:774	Since cork oak (Quercus suber) phellogen is seasonally activated and its proliferation and specification to phellem cells is a continuous developmental process, the differentially expressed genes during the cork seasonal growth served us to identify molecular processes embracing from phellogen to mature differentiated phellem cell.
34103550	2	24	theme	wounded	339:345	arg1	tissues					347:353	wounded tissues	339:353	wounded tissues	339:353	Despite the vital importance of phellem in protecting the radially-growing plant organs and wounded tissues, practically only the suberin biosynthetic process has been studied molecularly so far.
34103550	3	25	theme	cork	449:452	arg1	phellogen					474:482	cork oak (Quercus suber) phellogen	449:482	cork oak (Quercus suber) phellogen	449:482	Since cork oak (Quercus suber) phellogen is seasonally activated and its proliferation and specification to phellem cells is a continuous developmental process, the differentially expressed genes during the cork seasonal growth served us to identify molecular processes embracing from phellogen to mature differentiated phellem cell.
34103550	0	26	theme	Transcriptomic	0:13	arg1	analysis					15:22	Transcriptomic analysis	0:22	Transcriptomic analysis of cork during seasonal growth	0:53	Transcriptomic analysis of cork during seasonal growth highlights regulatory and developmental processes from phellogen to phellem formation.
34103550	5	27	theme	suberin	1186:1192	arg1	biosynthesis					1170:1181	the biosynthesis	1166:1181	the biosynthesis of suberin, lignin, triterpenes and soluble aromatic compounds	1166:1244	Instead, at maximum (June) and advanced (July) cork growth, metabolic processes paralleling the phellem cell chemical composition, such as the biosynthesis of suberin, lignin, triterpenes and soluble aromatic compounds, were upregulated.
34103550	5	28	theme	cork	1074:1077	arg1	growth					1079:1084	cork growth	1074:1084	cork growth	1074:1084	Instead, at maximum (June) and advanced (July) cork growth, metabolic processes paralleling the phellem cell chemical composition, such as the biosynthesis of suberin, lignin, triterpenes and soluble aromatic compounds, were upregulated.
34103550	4	29	theme	growth	802:807	arg1	beginning					784:792	the beginning	780:792	the beginning of cork growth (April)	780:815	At the beginning of cork growth (April), cell cycle regulation, meristem proliferation and maintenance and processes triggering cell differentiation were upregulated, showing an enrichment of phellogenic cells from which phellem cells are specified.
34103550	3	30	theme	oak	454:456	arg1	phellogen					474:482	cork oak (Quercus suber) phellogen	449:482	cork oak (Quercus suber) phellogen	449:482	Since cork oak (Quercus suber) phellogen is seasonally activated and its proliferation and specification to phellem cells is a continuous developmental process, the differentially expressed genes during the cork seasonal growth served us to identify molecular processes embracing from phellogen to mature differentiated phellem cell.
34103550	0	31	theme	cork	27:30	arg1	analysis					15:22	Transcriptomic analysis	0:22	Transcriptomic analysis of cork during seasonal growth	0:53	Transcriptomic analysis of cork during seasonal growth highlights regulatory and developmental processes from phellogen to phellem formation.
34103550	4	32	theme	meristem	841:848	arg1	proliferation					850:862	meristem proliferation	841:862	meristem proliferation	841:862	At the beginning of cork growth (April), cell cycle regulation, meristem proliferation and maintenance and processes triggering cell differentiation were upregulated, showing an enrichment of phellogenic cells from which phellem cells are specified.
34103550	3	33	theme	continuous	570:579	arg1	specification					534:546	specification	534:546	specification	534:546	Since cork oak (Quercus suber) phellogen is seasonally activated and its proliferation and specification to phellem cells is a continuous developmental process, the differentially expressed genes during the cork seasonal growth served us to identify molecular processes embracing from phellogen to mature differentiated phellem cell.
34103550	3	33	theme	continuous	570:579	arg1	process					595:601	a continuous developmental process	568:601	a continuous developmental process	568:601	Since cork oak (Quercus suber) phellogen is seasonally activated and its proliferation and specification to phellem cells is a continuous developmental process, the differentially expressed genes during the cork seasonal growth served us to identify molecular processes embracing from phellogen to mature differentiated phellem cell.
34103550	3	33	theme	continuous	570:579	arg1	proliferation					516:528	its proliferation	512:528	its proliferation	512:528	Since cork oak (Quercus suber) phellogen is seasonally activated and its proliferation and specification to phellem cells is a continuous developmental process, the differentially expressed genes during the cork seasonal growth served us to identify molecular processes embracing from phellogen to mature differentiated phellem cell.
34103550	5	34	dep	maximum	1039:1045	arg1	growth					1079:1084	cork growth	1074:1084	cork growth	1074:1084	Instead, at maximum (June) and advanced (July) cork growth, metabolic processes paralleling the phellem cell chemical composition, such as the biosynthesis of suberin, lignin, triterpenes and soluble aromatic compounds, were upregulated.
34103550	6	35	theme	formation	1450:1458	arg1	stages					1435:1440	the later stages	1425:1440	the later stages	1425:1440	Particularly in July, polysaccharides- and lignin-related secondary cell wall processes presented a maximal expression, indicating a cell wall reinforcement in the later stages of cork formation, presumably related with the initiation of latecork development.
34103550	5	36	theme	lignin	1195:1200	arg1	biosynthesis					1170:1181	the biosynthesis	1166:1181	the biosynthesis of suberin, lignin, triterpenes and soluble aromatic compounds	1166:1244	Instead, at maximum (June) and advanced (July) cork growth, metabolic processes paralleling the phellem cell chemical composition, such as the biosynthesis of suberin, lignin, triterpenes and soluble aromatic compounds, were upregulated.
34103550	6	37	theme	maximal	1365:1371	arg1	expression					1373:1382	a maximal expression	1363:1382	a maximal expression	1363:1382	Particularly in July, polysaccharides- and lignin-related secondary cell wall processes presented a maximal expression, indicating a cell wall reinforcement in the later stages of cork formation, presumably related with the initiation of latecork development.
34103550	2	38	theme	plant	322:326	arg1	organs					328:333	the radially-growing plant organs	301:333	the radially-growing plant organs	301:333	Despite the vital importance of phellem in protecting the radially-growing plant organs and wounded tissues, practically only the suberin biosynthetic process has been studied molecularly so far.
34103550	0	39	theme	seasonal	39:46	arg1	growth					48:53	seasonal growth	39:53	seasonal growth	39:53	Transcriptomic analysis of cork during seasonal growth highlights regulatory and developmental processes from phellogen to phellem formation.
34103550	4	40	theme	cells	981:985	arg1	enrichment					955:964	an enrichment	952:964	an enrichment of phellogenic cells from which phellem cells are specified	952:1024	At the beginning of cork growth (April), cell cycle regulation, meristem proliferation and maintenance and processes triggering cell differentiation were upregulated, showing an enrichment of phellogenic cells from which phellem cells are specified.
34103550	3	41	theme	expressed	623:631	arg1	genes					633:637	the differentially expressed genes	604:637	the differentially expressed genes during the cork seasonal growth	604:669	Since cork oak (Quercus suber) phellogen is seasonally activated and its proliferation and specification to phellem cells is a continuous developmental process, the differentially expressed genes during the cork seasonal growth served us to identify molecular processes embracing from phellogen to mature differentiated phellem cell.
34103550	7	42	theme	relevant	1550:1557	arg1	genes					1559:1563	relevant genes	1550:1563	relevant genes identified	1550:1574	The putative function of relevant genes identified are discussed in the context of phellem ontogeny.
34103550	3	43	theme	suber	467:471	arg1	phellogen					474:482	cork oak (Quercus suber) phellogen	449:482	cork oak (Quercus suber) phellogen	449:482	Since cork oak (Quercus suber) phellogen is seasonally activated and its proliferation and specification to phellem cells is a continuous developmental process, the differentially expressed genes during the cork seasonal growth served us to identify molecular processes embracing from phellogen to mature differentiated phellem cell.
34103550	4	44	theme	phellogenic	969:979	arg1	cells					981:985	phellogenic cells	969:985	phellogenic cells from which phellem cells are specified	969:1024	At the beginning of cork growth (April), cell cycle regulation, meristem proliferation and maintenance and processes triggering cell differentiation were upregulated, showing an enrichment of phellogenic cells from which phellem cells are specified.
34103550	5	45	theme	metabolic	1087:1095	arg1	biosynthesis					1170:1181	the biosynthesis	1166:1181	the biosynthesis of suberin, lignin, triterpenes and soluble aromatic compounds	1166:1244	Instead, at maximum (June) and advanced (July) cork growth, metabolic processes paralleling the phellem cell chemical composition, such as the biosynthesis of suberin, lignin, triterpenes and soluble aromatic compounds, were upregulated.
34103550	5	45	theme	metabolic	1087:1095	arg1	processes					1097:1105	metabolic processes	1087:1105	metabolic processes paralleling the phellem cell chemical composition	1087:1155	Instead, at maximum (June) and advanced (July) cork growth, metabolic processes paralleling the phellem cell chemical composition, such as the biosynthesis of suberin, lignin, triterpenes and soluble aromatic compounds, were upregulated.
34103550	6	46	theme	secondary	1323:1331	arg1	processes					1343:1351	polysaccharides- and lignin-related secondary cell wall processes	1287:1351	polysaccharides- and lignin-related secondary cell wall processes	1287:1351	Particularly in July, polysaccharides- and lignin-related secondary cell wall processes presented a maximal expression, indicating a cell wall reinforcement in the later stages of cork formation, presumably related with the initiation of latecork development.
34103550	5	47	theme	phellem	1123:1129	arg1	composition					1145:1155	the phellem cell chemical composition	1119:1155	the phellem cell chemical composition	1119:1155	Instead, at maximum (June) and advanced (July) cork growth, metabolic processes paralleling the phellem cell chemical composition, such as the biosynthesis of suberin, lignin, triterpenes and soluble aromatic compounds, were upregulated.
34103550	6	48	theme	later	1429:1433	arg1	stages					1435:1440	the later stages	1425:1440	the later stages	1425:1440	Particularly in July, polysaccharides- and lignin-related secondary cell wall processes presented a maximal expression, indicating a cell wall reinforcement in the later stages of cork formation, presumably related with the initiation of latecork development.
34103550	6	49	theme	wall	1338:1341	arg1	processes					1343:1351	polysaccharides- and lignin-related secondary cell wall processes	1287:1351	polysaccharides- and lignin-related secondary cell wall processes	1287:1351	Particularly in July, polysaccharides- and lignin-related secondary cell wall processes presented a maximal expression, indicating a cell wall reinforcement in the later stages of cork formation, presumably related with the initiation of latecork development.
34103550	1	50	dep	that divide	183:193	arg1	specify					222:228	specify	222:228	that divide periclinally and outwardly specify phellem or cork	183:244	The phellogen or cork cambium stem cells that divide periclinally and outwardly specify phellem or cork.
34103550	7	51	theme	genes	1559:1563	arg1	function					1538:1545	The putative function	1525:1545	The putative function of relevant genes identified	1525:1574	The putative function of relevant genes identified are discussed in the context of phellem ontogeny.
34103550	4	52	theme	cell	818:821	arg1	regulation					829:838	cell cycle regulation	818:838	cell cycle regulation	818:838	At the beginning of cork growth (April), cell cycle regulation, meristem proliferation and maintenance and processes triggering cell differentiation were upregulated, showing an enrichment of phellogenic cells from which phellem cells are specified.
34103550	6	53	theme	cell	1333:1336	arg1	processes					1343:1351	polysaccharides- and lignin-related secondary cell wall processes	1287:1351	polysaccharides- and lignin-related secondary cell wall processes	1287:1351	Particularly in July, polysaccharides- and lignin-related secondary cell wall processes presented a maximal expression, indicating a cell wall reinforcement in the later stages of cork formation, presumably related with the initiation of latecork development.
34103550	3	54	theme	cork	650:653	arg1	growth					664:669	the cork seasonal growth	646:669	the cork seasonal growth	646:669	Since cork oak (Quercus suber) phellogen is seasonally activated and its proliferation and specification to phellem cells is a continuous developmental process, the differentially expressed genes during the cork seasonal growth served us to identify molecular processes embracing from phellogen to mature differentiated phellem cell.
34103550	2	55	theme	phellem	279:285	arg1	importance					265:274	the vital importance	255:274	the vital importance of phellem in protecting the radially-growing plant organs and wounded tissues	255:353	Despite the vital importance of phellem in protecting the radially-growing plant organs and wounded tissues, practically only the suberin biosynthetic process has been studied molecularly so far.
34103550	5	56	theme	compounds	1236:1244	arg1	biosynthesis					1170:1181	the biosynthesis	1166:1181	the biosynthesis of suberin, lignin, triterpenes and soluble aromatic compounds	1166:1244	Instead, at maximum (June) and advanced (July) cork growth, metabolic processes paralleling the phellem cell chemical composition, such as the biosynthesis of suberin, lignin, triterpenes and soluble aromatic compounds, were upregulated.
34103550	6	57	theme	wall	1403:1406	arg1	reinforcement					1408:1420	a cell wall reinforcement	1396:1420	a cell wall reinforcement	1396:1420	Particularly in July, polysaccharides- and lignin-related secondary cell wall processes presented a maximal expression, indicating a cell wall reinforcement in the later stages of cork formation, presumably related with the initiation of latecork development.
34103550	7	58	theme	putative	1529:1536	arg1	function					1538:1545	The putative function	1525:1545	The putative function of relevant genes identified	1525:1574	The putative function of relevant genes identified are discussed in the context of phellem ontogeny.
34103550	3	59	theme	seasonal	655:662	arg1	growth					664:669	the cork seasonal growth	646:669	the cork seasonal growth	646:669	Since cork oak (Quercus suber) phellogen is seasonally activated and its proliferation and specification to phellem cells is a continuous developmental process, the differentially expressed genes during the cork seasonal growth served us to identify molecular processes embracing from phellogen to mature differentiated phellem cell.
34103550	1	60	theme	cork	159:162	arg1	cells					177:181	The phellogen or cork cambium stem cells	142:181	cells	177:181	The phellogen or cork cambium stem cells that divide periclinally and outwardly specify phellem or cork.
34103550	6	61	theme	development	1512:1522	arg1	initiation					1489:1498	the initiation	1485:1498	the initiation of latecork development	1485:1522	Particularly in July, polysaccharides- and lignin-related secondary cell wall processes presented a maximal expression, indicating a cell wall reinforcement in the later stages of cork formation, presumably related with the initiation of latecork development.
34103550	3	62	theme	molecular	693:701	arg1	processes					703:711	molecular processes	693:711	molecular processes embracing from phellogen to mature differentiated phellem cell	693:774	Since cork oak (Quercus suber) phellogen is seasonally activated and its proliferation and specification to phellem cells is a continuous developmental process, the differentially expressed genes during the cork seasonal growth served us to identify molecular processes embracing from phellogen to mature differentiated phellem cell.
34103550	4	63	theme	cycle	823:827	arg1	regulation					829:838	cell cycle regulation	818:838	cell cycle regulation	818:838	At the beginning of cork growth (April), cell cycle regulation, meristem proliferation and maintenance and processes triggering cell differentiation were upregulated, showing an enrichment of phellogenic cells from which phellem cells are specified.
34103550	6	64	theme	latecork	1503:1510	arg1	development					1512:1522	latecork development	1503:1522	latecork development	1503:1522	Particularly in July, polysaccharides- and lignin-related secondary cell wall processes presented a maximal expression, indicating a cell wall reinforcement in the later stages of cork formation, presumably related with the initiation of latecork development.
34103550	7	65	theme	phellem	1608:1614	arg1	ontogeny					1616:1623	phellem ontogeny	1608:1623	phellem ontogeny	1608:1623	The putative function of relevant genes identified are discussed in the context of phellem ontogeny.
34103550	0	66	theme	regulatory	66:75	arg1	processes					95:103	regulatory and developmental processes	66:103	regulatory and developmental processes	66:103	Transcriptomic analysis of cork during seasonal growth highlights regulatory and developmental processes from phellogen to phellem formation.
34103550	5	67	theme	chemical	1136:1143	arg1	composition					1145:1155	the phellem cell chemical composition	1119:1155	the phellem cell chemical composition	1119:1155	Instead, at maximum (June) and advanced (July) cork growth, metabolic processes paralleling the phellem cell chemical composition, such as the biosynthesis of suberin, lignin, triterpenes and soluble aromatic compounds, were upregulated.
34103550	2	68	theme	vital	259:263	arg1	importance					265:274	the vital importance	255:274	the vital importance of phellem in protecting the radially-growing plant organs and wounded tissues	255:353	Despite the vital importance of phellem in protecting the radially-growing plant organs and wounded tissues, practically only the suberin biosynthetic process has been studied molecularly so far.
34103550	2	69	theme	biosynthetic	385:396	arg1	process					398:404	practically only the suberin biosynthetic process	356:404	practically only the suberin biosynthetic process	356:404	Despite the vital importance of phellem in protecting the radially-growing plant organs and wounded tissues, practically only the suberin biosynthetic process has been studied molecularly so far.
34103550	7	70	theme	ontogeny	1616:1623	arg1	context					1597:1603	the context	1593:1603	the context of phellem ontogeny	1593:1623	The putative function of relevant genes identified are discussed in the context of phellem ontogeny.
34098262	0	0	theme	ionic	98:102	arg1	pollutants					132:141	ionic and non-ionic toxic organic pollutants	98:141	ionic and non-ionic toxic organic pollutants from water	98:152	β-cyclodextrin-containing polymer based on renewable cellulose resources for effective removal of ionic and non-ionic toxic organic pollutants from water.
34098262	8	1	theme	promising	1383:1391	arg1	potential					1393:1401	a promising potential	1381:1401	a promising potential for practical applications	1381:1428	Furthermore, almost complete removal of the pollutants was observed during the treatment of real effluents samples thus the bio-derived, cheap and reusable BAN-EPI-CDP has a promising potential for practical applications.
34098262	1	2	theme	simultaneous	278:289	arg1	removal					291:297	efficient, rapid and simultaneous removal	257:297	efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water	257:404	A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water.
34098262	6	3	theme	Langmuir	983:990	arg1	model					1001:1005	Langmuir isotherm model	983:1005	Langmuir isotherm model	983:1005	According to Langmuir isotherm model, the maximum adsorption capacities were found at 113.64 and 43.10 mg g-1 for BPA and CV, respectively.
34098262	2	4	theme	groups	466:471	arg1	composition					411:421	composition	411:421	composition	411:421	The composition, morphology and the presence of functional groups in the obtained sorption material were characterized by elemental analysis, XRD, SEM, and FTIR spectroscopy.
34098262	2	4	theme	groups	466:471	arg1	morphology					424:433	morphology	424:433	morphology	424:433	The composition, morphology and the presence of functional groups in the obtained sorption material were characterized by elemental analysis, XRD, SEM, and FTIR spectroscopy.
34098262	2	4	theme	groups	466:471	arg1	presence					443:450	the presence	439:450	the presence	439:450	The composition, morphology and the presence of functional groups in the obtained sorption material were characterized by elemental analysis, XRD, SEM, and FTIR spectroscopy.
34098262	4	5	theme	pollutants	765:774	arg1	processes					742:750	The sorption processes	729:750	The sorption processes of the model pollutants	729:774	The sorption processes of the model pollutants were studied with both kinetic and equilibrium models.
34098262	0	6	theme	non-ionic	108:116	arg1	pollutants					132:141	ionic and non-ionic toxic organic pollutants	98:141	ionic and non-ionic toxic organic pollutants from water	98:152	β-cyclodextrin-containing polymer based on renewable cellulose resources for effective removal of ionic and non-ionic toxic organic pollutants from water.
34098262	1	7	theme	non-ionic	366:374	arg1	pollutants					384:393	non-ionic organic pollutants	366:393	non-ionic organic pollutants	366:393	A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water.
34098262	8	8	theme	reusable	1356:1363	arg1	BAN-EPI-CDP					1365:1375	the bio-derived, cheap and reusable BAN-EPI-CDP	1329:1375	the bio-derived, cheap and reusable BAN-EPI-CDP	1329:1375	Furthermore, almost complete removal of the pollutants was observed during the treatment of real effluents samples thus the bio-derived, cheap and reusable BAN-EPI-CDP has a promising potential for practical applications.
34098262	5	9	theme	order	957:961	arg1	model					963:967	a pseudo-second order model	941:967	a pseudo-second order model	941:967	The results showed that the sorption was rapid (less than 1 min) and the time evolution could be fitted using a pseudo-second order model.
34098262	1	10	theme	organic	376:382	arg1	pollutants					384:393	non-ionic organic pollutants	366:393	non-ionic organic pollutants	366:393	A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water.
34098262	7	11	theme	regeneration	1188:1199	arg1	cycles					1201:1206	five regeneration cycles	1183:1206	five regeneration cycles	1183:1206	The adsorption ability of β-CDPs was kept nearly on the same level after five regeneration cycles.
34098262	3	12	dep	compound	684:691	arg1	A					704:704	bisphenol A	694:704	bisphenol A	694:704	The adsorption results were represented by cationic dye (crystal violet, CV) and endocrine disrupting compound (bisphenol A, BPA) as an adsorbate.
34098262	3	12	dep	compound	684:691	arg1	BPA					707:709	BPA	707:709	BPA	707:709	The adsorption results were represented by cationic dye (crystal violet, CV) and endocrine disrupting compound (bisphenol A, BPA) as an adsorbate.
34098262	0	13	theme	organic	124:130	arg1	pollutants					132:141	ionic and non-ionic toxic organic pollutants	98:141	ionic and non-ionic toxic organic pollutants from water	98:152	β-cyclodextrin-containing polymer based on renewable cellulose resources for effective removal of ionic and non-ionic toxic organic pollutants from water.
34098262	1	14	theme	pollutants	384:393	arg1	broad-spectrum					304:317	a broad-spectrum	302:317	a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water	302:404	A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water.
34098262	4	15	theme	model	759:763	arg1	pollutants					765:774	the model pollutants	755:774	the model pollutants	755:774	The sorption processes of the model pollutants were studied with both kinetic and equilibrium models.
34098262	1	16	theme	cyclodextrin-based	176:193	arg1	trifunctional					195:207	A novel, bio-derived cyclodextrin-based trifunctional	155:207	A novel, bio-derived cyclodextrin-based trifunctional adsorbent	155:217	A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water.
34098262	0	17	theme	toxic	118:122	arg1	pollutants					132:141	ionic and non-ionic toxic organic pollutants	98:141	ionic and non-ionic toxic organic pollutants from water	98:152	β-cyclodextrin-containing polymer based on renewable cellulose resources for effective removal of ionic and non-ionic toxic organic pollutants from water.
34098262	8	18	dep	has	1377:1379	arg1	observed					1268:1275	observed	1268:1275	was observed during the treatment of real effluents samples	1264:1322	Furthermore, almost complete removal of the pollutants was observed during the treatment of real effluents samples thus the bio-derived, cheap and reusable BAN-EPI-CDP has a promising potential for practical applications.
34098262	1	19	from	water	400:404	arg1	broad-spectrum					304:317	a broad-spectrum	302:317	a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water	302:404	A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water.
34098262	1	19	from	water	400:404	arg1	ionic					328:332	ionic	328:332	ionic	328:332	A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water.
34098262	1	19	from	water	400:404	arg1	dyes					356:359	anionic and cationic dyes	335:359	anionic and cationic dyes	335:359	A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water.
34098262	7	20	theme	same	1166:1169	arg1	level					1171:1175	the same level	1162:1175	the same level	1162:1175	The adsorption ability of β-CDPs was kept nearly on the same level after five regeneration cycles.
34098262	1	21	from	broad-spectrum	304:317	arg1	water					400:404	water	400:404	water	400:404	A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water.
34098262	6	22	located	found	1047:1051	arg2	capacities					1031:1040	the maximum adsorption capacities	1008:1040	the maximum adsorption capacities	1008:1040	According to Langmuir isotherm model, the maximum adsorption capacities were found at 113.64 and 43.10 mg g-1 for BPA and CV, respectively.
34098262	6	22	located	found	1047:1051	arg1	43.10 mg g-1					1067:1078	43.10 mg g-1	1067:1078	43.10 mg g-1	1067:1078	According to Langmuir isotherm model, the maximum adsorption capacities were found at 113.64 and 43.10 mg g-1 for BPA and CV, respectively.
34098262	6	22	located	found	1047:1051	arg1	113.64					1056:1061	113.64	1056:1061	113.64	1056:1061	According to Langmuir isotherm model, the maximum adsorption capacities were found at 113.64 and 43.10 mg g-1 for BPA and CV, respectively.
34098262	1	23	theme	adsorbent	209:217	arg1	trifunctional					195:207	A novel, bio-derived cyclodextrin-based trifunctional	155:207	A novel, bio-derived cyclodextrin-based trifunctional adsorbent	155:217	A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water.
34098262	0	24	theme	β-cyclodextrin-containing	0:24	arg1	polymer					26:32	β-cyclodextrin-containing polymer	0:32	β-cyclodextrin-containing polymer	0:32	β-cyclodextrin-containing polymer based on renewable cellulose resources for effective removal of ionic and non-ionic toxic organic pollutants from water.
34098262	1	25	theme	broad-spectrum	304:317	arg1	removal					291:297	efficient, rapid and simultaneous removal	257:297	efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water	257:404	A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water.
34098262	3	26	theme	adsorption	586:595	arg1	results					597:603	The adsorption results	582:603	The adsorption results	582:603	The adsorption results were represented by cationic dye (crystal violet, CV) and endocrine disrupting compound (bisphenol A, BPA) as an adsorbate.
34098262	3	26	theme	adsorption	586:595	arg1	adsorbate					718:726	an adsorbate	715:726	an adsorbate	715:726	The adsorption results were represented by cationic dye (crystal violet, CV) and endocrine disrupting compound (bisphenol A, BPA) as an adsorbate.
34098262	8	27	theme	effluents	1306:1314	arg1	samples					1316:1322	real effluents samples	1301:1322	real effluents samples	1301:1322	Furthermore, almost complete removal of the pollutants was observed during the treatment of real effluents samples thus the bio-derived, cheap and reusable BAN-EPI-CDP has a promising potential for practical applications.
34098262	6	28	theme	adsorption	1020:1029	arg1	capacities					1031:1040	the maximum adsorption capacities	1008:1040	the maximum adsorption capacities	1008:1040	According to Langmuir isotherm model, the maximum adsorption capacities were found at 113.64 and 43.10 mg g-1 for BPA and CV, respectively.
34098262	2	29	theme	functional	455:464	arg1	groups					466:471	functional groups	455:471	functional groups	455:471	The composition, morphology and the presence of functional groups in the obtained sorption material were characterized by elemental analysis, XRD, SEM, and FTIR spectroscopy.
34098262	8	30	theme	pollutants	1253:1262	arg1	removal					1238:1244	almost complete removal	1222:1244	almost complete removal of the pollutants	1222:1262	Furthermore, almost complete removal of the pollutants was observed during the treatment of real effluents samples thus the bio-derived, cheap and reusable BAN-EPI-CDP has a promising potential for practical applications.
34098262	2	31	theme	FTIR	563:566	arg1	spectroscopy					568:579	FTIR spectroscopy	563:579	FTIR spectroscopy	563:579	The composition, morphology and the presence of functional groups in the obtained sorption material were characterized by elemental analysis, XRD, SEM, and FTIR spectroscopy.
34098262	4	32	theme	sorption	733:740	arg1	processes					742:750	The sorption processes	729:750	The sorption processes of the model pollutants	729:774	The sorption processes of the model pollutants were studied with both kinetic and equilibrium models.
34098262	6	33	theme	maximum	1012:1018	arg1	capacities					1031:1040	the maximum adsorption capacities	1008:1040	the maximum adsorption capacities	1008:1040	According to Langmuir isotherm model, the maximum adsorption capacities were found at 113.64 and 43.10 mg g-1 for BPA and CV, respectively.
34098262	5	34	theme	time	904:907	arg1	evolution					909:917	the time evolution	900:917	the time evolution	900:917	The results showed that the sorption was rapid (less than 1 min) and the time evolution could be fitted using a pseudo-second order model.
34098262	0	35	theme	pollutants	132:141	arg1	removal					87:93	effective removal	77:93	effective removal of ionic and non-ionic toxic organic pollutants from water	77:152	β-cyclodextrin-containing polymer based on renewable cellulose resources for effective removal of ionic and non-ionic toxic organic pollutants from water.
34098262	1	36	dep	novel	157:161	arg1	bio-derived					164:174	bio-derived	164:174	bio-derived	164:174	A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water.
34098262	3	37	dep	dye	634:636	arg1	CV					655:656	CV	655:656	CV	655:656	The adsorption results were represented by cationic dye (crystal violet, CV) and endocrine disrupting compound (bisphenol A, BPA) as an adsorbate.
34098262	3	37	dep	dye	634:636	arg1	violet					647:652	crystal violet	639:652	crystal violet	639:652	The adsorption results were represented by cationic dye (crystal violet, CV) and endocrine disrupting compound (bisphenol A, BPA) as an adsorbate.
34098262	0	38	theme	cellulose	53:61	arg1	resources					63:71	renewable cellulose resources	43:71	renewable cellulose resources for effective removal of ionic and non-ionic toxic organic pollutants from water	43:152	β-cyclodextrin-containing polymer based on renewable cellulose resources for effective removal of ionic and non-ionic toxic organic pollutants from water.
34098262	1	39	theme	ionic	328:332	arg1	broad-spectrum					304:317	a broad-spectrum	302:317	a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water	302:404	A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water.
34098262	2	40	attach	presence	443:450	arg1	material					498:505	the obtained sorption material	476:505	the obtained sorption material	476:505	The composition, morphology and the presence of functional groups in the obtained sorption material were characterized by elemental analysis, XRD, SEM, and FTIR spectroscopy.
34098262	2	40	attach	presence	443:450	arg2	groups					466:471	functional groups	455:471	functional groups	455:471	The composition, morphology and the presence of functional groups in the obtained sorption material were characterized by elemental analysis, XRD, SEM, and FTIR spectroscopy.
34098262	8	41	theme	cheap	1346:1350	arg1	BAN-EPI-CDP					1365:1375	the bio-derived, cheap and reusable BAN-EPI-CDP	1329:1375	the bio-derived, cheap and reusable BAN-EPI-CDP	1329:1375	Furthermore, almost complete removal of the pollutants was observed during the treatment of real effluents samples thus the bio-derived, cheap and reusable BAN-EPI-CDP has a promising potential for practical applications.
34098262	8	42	contain	has	1377:1379	arg2	potential					1393:1401	a promising potential	1381:1401	a promising potential for practical applications	1381:1428	Furthermore, almost complete removal of the pollutants was observed during the treatment of real effluents samples thus the bio-derived, cheap and reusable BAN-EPI-CDP has a promising potential for practical applications.
34098262	8	42	contain	has	1377:1379	arg1	BAN-EPI-CDP					1365:1375	the bio-derived, cheap and reusable BAN-EPI-CDP	1329:1375	the bio-derived, cheap and reusable BAN-EPI-CDP	1329:1375	Furthermore, almost complete removal of the pollutants was observed during the treatment of real effluents samples thus the bio-derived, cheap and reusable BAN-EPI-CDP has a promising potential for practical applications.
34098262	0	43	theme	renewable	43:51	arg1	resources					63:71	renewable cellulose resources	43:71	renewable cellulose resources for effective removal of ionic and non-ionic toxic organic pollutants from water	43:152	β-cyclodextrin-containing polymer based on renewable cellulose resources for effective removal of ionic and non-ionic toxic organic pollutants from water.
34098262	8	44	link	bio-derived	1333:1343	arg1	BAN-EPI-CDP					1365:1375	the bio-derived, cheap and reusable BAN-EPI-CDP	1329:1375	the bio-derived, cheap and reusable BAN-EPI-CDP	1329:1375	Furthermore, almost complete removal of the pollutants was observed during the treatment of real effluents samples thus the bio-derived, cheap and reusable BAN-EPI-CDP has a promising potential for practical applications.
34098262	2	45	theme	sorption	489:496	arg1	material					498:505	the obtained sorption material	476:505	the obtained sorption material	476:505	The composition, morphology and the presence of functional groups in the obtained sorption material were characterized by elemental analysis, XRD, SEM, and FTIR spectroscopy.
34098262	0	46	from	removal	87:93	arg1	water					148:152	water	148:152	water	148:152	β-cyclodextrin-containing polymer based on renewable cellulose resources for effective removal of ionic and non-ionic toxic organic pollutants from water.
34098262	7	47	theme	adsorption	1114:1123	arg1	ability					1125:1131	The adsorption ability	1110:1131	The adsorption ability of β-CDPs	1110:1141	The adsorption ability of β-CDPs was kept nearly on the same level after five regeneration cycles.
34098262	2	48	theme	obtained	480:487	arg1	material					498:505	the obtained sorption material	476:505	the obtained sorption material	476:505	The composition, morphology and the presence of functional groups in the obtained sorption material were characterized by elemental analysis, XRD, SEM, and FTIR spectroscopy.
34098262	1	49	theme	anionic	335:341	arg1	dyes					356:359	anionic and cationic dyes	335:359	anionic and cationic dyes	335:359	A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water.
34098262	1	49	theme	anionic	335:341	arg1	ionic					328:332	ionic	328:332	ionic	328:332	A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water.
34098262	8	50	theme	practical	1407:1415	arg1	applications					1417:1428	practical applications	1407:1428	practical applications	1407:1428	Furthermore, almost complete removal of the pollutants was observed during the treatment of real effluents samples thus the bio-derived, cheap and reusable BAN-EPI-CDP has a promising potential for practical applications.
34098262	4	51	theme	equilibrium	811:821	arg1	models					823:828	equilibrium models	811:828	equilibrium models	811:828	The sorption processes of the model pollutants were studied with both kinetic and equilibrium models.
34098262	8	52	theme	samples	1316:1322	arg1	treatment					1288:1296	the treatment	1284:1296	the treatment of real effluents samples	1284:1322	Furthermore, almost complete removal of the pollutants was observed during the treatment of real effluents samples thus the bio-derived, cheap and reusable BAN-EPI-CDP has a promising potential for practical applications.
34098262	3	53	theme	bisphenol	694:702	arg1	A					704:704	bisphenol A	694:704	bisphenol A	694:704	The adsorption results were represented by cationic dye (crystal violet, CV) and endocrine disrupting compound (bisphenol A, BPA) as an adsorbate.
34098262	3	53	theme	bisphenol	694:702	arg1	BPA					707:709	BPA	707:709	BPA	707:709	The adsorption results were represented by cationic dye (crystal violet, CV) and endocrine disrupting compound (bisphenol A, BPA) as an adsorbate.
34098262	3	54	theme	disrupting	673:682	arg1	compound					684:691	endocrine disrupting compound	663:691	endocrine disrupting compound (bisphenol A, BPA)	663:710	The adsorption results were represented by cationic dye (crystal violet, CV) and endocrine disrupting compound (bisphenol A, BPA) as an adsorbate.
34098262	1	55	theme	cationic	347:354	arg1	dyes					356:359	anionic and cationic dyes	335:359	anionic and cationic dyes	335:359	A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water.
34098262	1	55	theme	cationic	347:354	arg1	ionic					328:332	ionic	328:332	ionic	328:332	A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water.
34098262	8	56	theme	complete	1229:1236	arg1	removal					1238:1244	almost complete removal	1222:1244	almost complete removal of the pollutants	1222:1262	Furthermore, almost complete removal of the pollutants was observed during the treatment of real effluents samples thus the bio-derived, cheap and reusable BAN-EPI-CDP has a promising potential for practical applications.
34098262	5	57	theme	pseudo-second	943:955	arg1	model					963:967	a pseudo-second order model	941:967	a pseudo-second order model	941:967	The results showed that the sorption was rapid (less than 1 min) and the time evolution could be fitted using a pseudo-second order model.
34098262	2	58	from	composition	411:421	arg1	material					498:505	the obtained sorption material	476:505	the obtained sorption material	476:505	The composition, morphology and the presence of functional groups in the obtained sorption material were characterized by elemental analysis, XRD, SEM, and FTIR spectroscopy.
34098262	5	59	dep	rapid	872:876	arg1	less					879:882	less	879:882	less	879:882	The results showed that the sorption was rapid (less than 1 min) and the time evolution could be fitted using a pseudo-second order model.
34098262	5	59	dep	rapid	872:876	arg1	rapid					872:876	rapid	872:876	rapid	872:876	The results showed that the sorption was rapid (less than 1 min) and the time evolution could be fitted using a pseudo-second order model.
34098262	5	59	dep	rapid	872:876	arg1	sorption					859:866	the sorption	855:866	the sorption	855:866	The results showed that the sorption was rapid (less than 1 min) and the time evolution could be fitted using a pseudo-second order model.
34098262	8	60	theme	bio-derived	1333:1343	arg1	BAN-EPI-CDP					1365:1375	the bio-derived, cheap and reusable BAN-EPI-CDP	1329:1375	the bio-derived, cheap and reusable BAN-EPI-CDP	1329:1375	Furthermore, almost complete removal of the pollutants was observed during the treatment of real effluents samples thus the bio-derived, cheap and reusable BAN-EPI-CDP has a promising potential for practical applications.
34098262	1	61	theme	efficient	257:265	arg1	removal					291:297	efficient, rapid and simultaneous removal	257:297	efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water	257:404	A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water.
34098262	8	62	theme	real	1301:1304	arg1	samples					1316:1322	real effluents samples	1301:1322	real effluents samples	1301:1322	Furthermore, almost complete removal of the pollutants was observed during the treatment of real effluents samples thus the bio-derived, cheap and reusable BAN-EPI-CDP has a promising potential for practical applications.
34098262	3	63	theme	crystal	639:645	arg1	CV					655:656	CV	655:656	CV	655:656	The adsorption results were represented by cationic dye (crystal violet, CV) and endocrine disrupting compound (bisphenol A, BPA) as an adsorbate.
34098262	3	63	theme	crystal	639:645	arg1	violet					647:652	crystal violet	639:652	crystal violet	639:652	The adsorption results were represented by cationic dye (crystal violet, CV) and endocrine disrupting compound (bisphenol A, BPA) as an adsorbate.
34098262	0	64	from	water	148:152	arg1	pollutants					132:141	ionic and non-ionic toxic organic pollutants	98:141	ionic and non-ionic toxic organic pollutants from water	98:152	β-cyclodextrin-containing polymer based on renewable cellulose resources for effective removal of ionic and non-ionic toxic organic pollutants from water.
34098262	0	64	from	water	148:152	arg1	removal					87:93	effective removal	77:93	effective removal of ionic and non-ionic toxic organic pollutants from water	77:152	β-cyclodextrin-containing polymer based on renewable cellulose resources for effective removal of ionic and non-ionic toxic organic pollutants from water.
34098262	2	65	theme	elemental	529:537	arg1	analysis					539:546	elemental analysis	529:546	elemental analysis	529:546	The composition, morphology and the presence of functional groups in the obtained sorption material were characterized by elemental analysis, XRD, SEM, and FTIR spectroscopy.
34098262	3	66	theme	endocrine	663:671	arg1	compound					684:691	endocrine disrupting compound	663:691	endocrine disrupting compound (bisphenol A, BPA)	663:710	The adsorption results were represented by cationic dye (crystal violet, CV) and endocrine disrupting compound (bisphenol A, BPA) as an adsorbate.
34098262	3	67	theme	cationic	625:632	arg1	dye					634:636	cationic dye	625:636	cationic dye (crystal violet, CV)	625:657	The adsorption results were represented by cationic dye (crystal violet, CV) and endocrine disrupting compound (bisphenol A, BPA) as an adsorbate.
34098262	2	68	from	morphology	424:433	arg1	material					498:505	the obtained sorption material	476:505	the obtained sorption material	476:505	The composition, morphology and the presence of functional groups in the obtained sorption material were characterized by elemental analysis, XRD, SEM, and FTIR spectroscopy.
34098262	6	69	theme	isotherm	992:999	arg1	model					1001:1005	Langmuir isotherm model	983:1005	Langmuir isotherm model	983:1005	According to Langmuir isotherm model, the maximum adsorption capacities were found at 113.64 and 43.10 mg g-1 for BPA and CV, respectively.
34098262	1	70	theme	rapid	268:272	arg1	removal					291:297	efficient, rapid and simultaneous removal	257:297	efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water	257:404	A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water.
34098262	0	71	theme	effective	77:85	arg1	removal					87:93	effective removal	77:93	effective removal of ionic and non-ionic toxic organic pollutants from water	77:152	β-cyclodextrin-containing polymer based on renewable cellulose resources for effective removal of ionic and non-ionic toxic organic pollutants from water.
34098262	2	72	dep	composition	411:421	arg1	The					407:409	The	407:409	The	407:409	The composition, morphology and the presence of functional groups in the obtained sorption material were characterized by elemental analysis, XRD, SEM, and FTIR spectroscopy.
34098262	2	73	from	presence	443:450	arg1	material					498:505	the obtained sorption material	476:505	the obtained sorption material	476:505	The composition, morphology and the presence of functional groups in the obtained sorption material were characterized by elemental analysis, XRD, SEM, and FTIR spectroscopy.
34098262	1	74	theme	novel	157:161	arg1	trifunctional					195:207	A novel, bio-derived cyclodextrin-based trifunctional	155:207	A novel, bio-derived cyclodextrin-based trifunctional adsorbent	155:217	A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water.
34098262	7	75	theme	β-CDPs	1136:1141	arg1	ability					1125:1131	The adsorption ability	1110:1131	The adsorption ability of β-CDPs	1110:1141	The adsorption ability of β-CDPs was kept nearly on the same level after five regeneration cycles.
33274228	0	0	theme	Finishing	143:151	arg1	Pigs					153:156	Finishing Pigs	143:156	Finishing Pigs	143:156	A Study on the Preparation of Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed and Its Feeding Efficiency in Finishing Pigs.
33274228	3	1	theme	control	632:638	arg1	group					640:644	the control group	628:644	the control group	628:644	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	8	2	theme	cob	1516:1518	arg1	feed					1520:1523	6% fermented maize cob feed	1497:1523	6% fermented maize cob feed	1497:1523	It could be concluded that the addition of 6% fermented maize cob feed to the diets of finishing pigs could promote their growth, improve their production performance and slaughter performance meat quality, and enhance their intestinal microecological balance and immunity.
33274228	8	3	theme	microecological	1690:1704	arg1	balance					1706:1712	their intestinal microecological balance	1673:1712	their intestinal microecological balance	1673:1712	It could be concluded that the addition of 6% fermented maize cob feed to the diets of finishing pigs could promote their growth, improve their production performance and slaughter performance meat quality, and enhance their intestinal microecological balance and immunity.
33274228	7	4	theme	cytokine	1339:1346	arg1	content					1348:1354	cytokine content	1339:1354	cytokine content	1339:1354	The cytokine content and gene expression of sIgA, IL-8, and TNF-α were found to be significantly increased (p < 0.05).
33274228	2	5	theme	protein	590:596	arg1	content					598:604	the protein content	586:604	the protein content	586:604	The results showed that the appearance, texture, and nutrient quality of maize cobs significantly improved after fermentation, the total number of bacteria was 4.5 × 1010 CFU/g, and the protein content was 7.1%.
33274228	2	5	theme	protein	590:596	arg1	%					613:613	7.1%	610:613	7.1%	610:613	The results showed that the appearance, texture, and nutrient quality of maize cobs significantly improved after fermentation, the total number of bacteria was 4.5 × 1010 CFU/g, and the protein content was 7.1%.
33274228	1	6	theme	maize	166:170	arg1	material					182:189	1000 g maize cob mixed material	159:189	1000 g maize cob mixed material	159:189	1000 g maize cob mixed material was synergistically fermented by adding 2.5% composite probiotics and 0.06-0.08% NSP (nonstarch polysaccharide) enzyme to prepare fermented feed, and its effectiveness as feed for fattening pigs was investigated.
33274228	3	7	theme	maize	676:680	arg1	group					704:708	the 6% fermented maize cob feed experimental group	659:708	the 6% fermented maize cob feed experimental group	659:708	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	8	8	theme	finishing	1541:1549	arg1	pigs					1551:1554	finishing pigs	1541:1554	finishing pigs	1541:1554	It could be concluded that the addition of 6% fermented maize cob feed to the diets of finishing pigs could promote their growth, improve their production performance and slaughter performance meat quality, and enhance their intestinal microecological balance and immunity.
33274228	1	9	theme	mixed	176:180	arg1	material					182:189	1000 g maize cob mixed material	159:189	1000 g maize cob mixed material	159:189	1000 g maize cob mixed material was synergistically fermented by adding 2.5% composite probiotics and 0.06-0.08% NSP (nonstarch polysaccharide) enzyme to prepare fermented feed, and its effectiveness as feed for fattening pigs was investigated.
33274228	3	10	theme	%	664:664	arg1	group					704:708	the 6% fermented maize cob feed experimental group	659:708	the 6% fermented maize cob feed experimental group	659:708	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	6	11	theme	VH/CD	1233:1237	arg1	ratio					1239:1243	VH/CD ratio	1233:1243	VH/CD ratio	1233:1243	The intestinal crypt depth, VH/CD ratio, and ileal mucosal immunity of the finishing pigs also significantly improved (p < 0.05).
33274228	7	12	dep	increased	1432:1440	arg1	<					1445:1445	p < 0.05	1443:1450	p < 0.05	1443:1450	The cytokine content and gene expression of sIgA, IL-8, and TNF-α were found to be significantly increased (p < 0.05).
33274228	1	13	dep	%	234:234	arg1	probiotics					246:255	composite probiotics	236:255	2.5% composite probiotics	231:255	1000 g maize cob mixed material was synergistically fermented by adding 2.5% composite probiotics and 0.06-0.08% NSP (nonstarch polysaccharide) enzyme to prepare fermented feed, and its effectiveness as feed for fattening pigs was investigated.
33274228	0	14	theme	Feeding	121:127	arg1	Efficiency					129:138	Its Feeding Efficiency	117:138	Its Feeding Efficiency in Finishing Pigs	117:156	A Study on the Preparation of Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed and Its Feeding Efficiency in Finishing Pigs.
33274228	1	15	theme	fattening	371:379	arg1	pigs					381:384	fattening pigs	371:384	fattening pigs	371:384	1000 g maize cob mixed material was synergistically fermented by adding 2.5% composite probiotics and 0.06-0.08% NSP (nonstarch polysaccharide) enzyme to prepare fermented feed, and its effectiveness as feed for fattening pigs was investigated.
33274228	5	16	theme	beneficial	966:975	arg1	bacteria					977:984	beneficial bacteria	966:984	beneficial bacteria including Lactobacillus in the intestines of the finishing pigs	966:1048	In addition, beneficial bacteria including Lactobacillus in the intestines of the finishing pigs significantly increased, and pathogenic bacteria including Escherichia coli in the intestines and feces were found to be significantly reduced (p < 0.05).
33274228	5	16	theme	beneficial	966:975	arg1	Lactobacillus					996:1008	Lactobacillus	996:1008	Lactobacillus	996:1008	In addition, beneficial bacteria including Lactobacillus in the intestines of the finishing pigs significantly increased, and pathogenic bacteria including Escherichia coli in the intestines and feces were found to be significantly reduced (p < 0.05).
33274228	2	17	theme	bacteria	551:558	arg1	number					541:546	the total number	531:546	the total number of bacteria	531:558	The results showed that the appearance, texture, and nutrient quality of maize cobs significantly improved after fermentation, the total number of bacteria was 4.5 × 1010 CFU/g, and the protein content was 7.1%.
33274228	2	17	theme	bacteria	551:558	arg1	1010 CFU/g					570:579	4.5 × 1010 CFU/g	564:579	4.5 × 1010 CFU/g	564:579	The results showed that the appearance, texture, and nutrient quality of maize cobs significantly improved after fermentation, the total number of bacteria was 4.5 × 1010 CFU/g, and the protein content was 7.1%.
33274228	3	18	theme	feed	686:689	arg1	group					704:708	the 6% fermented maize cob feed experimental group	659:708	the 6% fermented maize cob feed experimental group	659:708	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	6	19	theme	intestinal	1209:1218	arg1	depth					1226:1230	The intestinal crypt depth	1205:1230	The intestinal crypt depth	1205:1230	The intestinal crypt depth, VH/CD ratio, and ileal mucosal immunity of the finishing pigs also significantly improved (p < 0.05).
33274228	3	20	theme	daily	741:745	arg1	intake					752:757	daily feed intake	741:757	daily feed intake	741:757	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	0	21	theme	Nonstarch	44:52	arg1	Feed					108:111	Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed	30:111	Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed	30:111	A Study on the Preparation of Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed and Its Feeding Efficiency in Finishing Pigs.
33274228	2	22	theme	nutrient	457:464	arg1	quality					466:472	nutrient quality	457:472	nutrient quality	457:472	The results showed that the appearance, texture, and nutrient quality of maize cobs significantly improved after fermentation, the total number of bacteria was 4.5 × 1010 CFU/g, and the protein content was 7.1%.
33274228	3	23	theme	daily	760:764	arg1	gain					773:776	daily weight gain	760:776	daily weight gain	760:776	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	0	24	theme	Enzyme	69:74	arg1	Feed					108:111	Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed	30:111	Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed	30:111	A Study on the Preparation of Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed and Its Feeding Efficiency in Finishing Pigs.
33274228	5	25	theme	pigs	1045:1048	arg1	intestines					1017:1026	the intestines	1013:1026	the intestines of the finishing pigs	1013:1048	In addition, beneficial bacteria including Lactobacillus in the intestines of the finishing pigs significantly increased, and pathogenic bacteria including Escherichia coli in the intestines and feces were found to be significantly reduced (p < 0.05).
33274228	2	26	dep	appearance	432:441	arg1	the					428:430	the	428:430	the	428:430	The results showed that the appearance, texture, and nutrient quality of maize cobs significantly improved after fermentation, the total number of bacteria was 4.5 × 1010 CFU/g, and the protein content was 7.1%.
33274228	8	27	theme	slaughter	1625:1633	arg1	quality					1652:1658	their production performance and slaughter performance meat quality	1592:1658	quality	1652:1658	It could be concluded that the addition of 6% fermented maize cob feed to the diets of finishing pigs could promote their growth, improve their production performance and slaughter performance meat quality, and enhance their intestinal microecological balance and immunity.
33274228	3	28	theme	digestion	792:800	arg1	rate					802:805	nutrient digestion rate	783:805	nutrient digestion rate (p < 0.05)	783:816	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	3	28	theme	digestion	792:800	arg1	<					810:810	p < 0.05	808:815	p < 0.05	808:815	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	0	29	theme	Fermented	88:96	arg1	Feed					108:111	Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed	30:111	Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed	30:111	A Study on the Preparation of Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed and Its Feeding Efficiency in Finishing Pigs.
33274228	8	30	theme	meat	1647:1650	arg1	quality					1652:1658	their production performance and slaughter performance meat quality	1592:1658	quality	1652:1658	It could be concluded that the addition of 6% fermented maize cob feed to the diets of finishing pigs could promote their growth, improve their production performance and slaughter performance meat quality, and enhance their intestinal microecological balance and immunity.
33274228	2	31	theme	cobs	483:486	arg1	quality					466:472	nutrient quality	457:472	nutrient quality	457:472	The results showed that the appearance, texture, and nutrient quality of maize cobs significantly improved after fermentation, the total number of bacteria was 4.5 × 1010 CFU/g, and the protein content was 7.1%.
33274228	2	31	theme	cobs	483:486	arg1	texture					444:450	texture	444:450	texture	444:450	The results showed that the appearance, texture, and nutrient quality of maize cobs significantly improved after fermentation, the total number of bacteria was 4.5 × 1010 CFU/g, and the protein content was 7.1%.
33274228	2	31	theme	cobs	483:486	arg1	appearance					432:441	appearance	432:441	appearance	432:441	The results showed that the appearance, texture, and nutrient quality of maize cobs significantly improved after fermentation, the total number of bacteria was 4.5 × 1010 CFU/g, and the protein content was 7.1%.
33274228	0	32	theme	Cob	104:106	arg1	Feed					108:111	Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed	30:111	Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed	30:111	A Study on the Preparation of Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed and Its Feeding Efficiency in Finishing Pigs.
33274228	0	33	from	Preparation	15:25	arg1	Pigs					153:156	Finishing Pigs	143:156	Finishing Pigs	143:156	A Study on the Preparation of Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed and Its Feeding Efficiency in Finishing Pigs.
33274228	0	34	theme	Polysaccharide	54:67	arg1	Feed					108:111	Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed	30:111	Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed	30:111	A Study on the Preparation of Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed and Its Feeding Efficiency in Finishing Pigs.
33274228	7	35	dep	content	1348:1354	arg1	The					1335:1337	The	1335:1337	The	1335:1337	The cytokine content and gene expression of sIgA, IL-8, and TNF-α were found to be significantly increased (p < 0.05).
33274228	3	36	theme	conversion	835:844	arg1	<					855:855	p < 0.05	853:860	p < 0.05	853:860	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	3	36	theme	conversion	835:844	arg1	ratio					846:850	feed conversion ratio	830:850	feed conversion ratio (p < 0.05)	830:861	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	7	37	theme	TNF-α	1395:1399	arg1	content					1348:1354	cytokine content	1339:1354	cytokine content	1339:1354	The cytokine content and gene expression of sIgA, IL-8, and TNF-α were found to be significantly increased (p < 0.05).
33274228	7	37	theme	TNF-α	1395:1399	arg1	expression					1365:1374	gene expression	1360:1374	gene expression	1360:1374	The cytokine content and gene expression of sIgA, IL-8, and TNF-α were found to be significantly increased (p < 0.05).
33274228	4	38	theme	slaughter	890:898	arg1	performance					900:910	slaughter performance	890:910	slaughter performance	890:910	Most indicators including slaughter performance and meat quality significantly improved.
33274228	1	39	theme	polysaccharide	287:300	arg1	enzyme					303:308	NSP (nonstarch polysaccharide) enzyme	272:308	0.06-0.08% NSP (nonstarch polysaccharide) enzyme	261:308	1000 g maize cob mixed material was synergistically fermented by adding 2.5% composite probiotics and 0.06-0.08% NSP (nonstarch polysaccharide) enzyme to prepare fermented feed, and its effectiveness as feed for fattening pigs was investigated.
33274228	0	40	theme	Microbial	30:38	arg1	Feed					108:111	Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed	30:111	Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed	30:111	A Study on the Preparation of Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed and Its Feeding Efficiency in Finishing Pigs.
33274228	7	41	theme	gene	1360:1363	arg1	expression					1365:1374	gene expression	1360:1374	gene expression	1360:1374	The cytokine content and gene expression of sIgA, IL-8, and TNF-α were found to be significantly increased (p < 0.05).
33274228	8	42	theme	intestinal	1679:1688	arg1	balance					1706:1712	their intestinal microecological balance	1673:1712	their intestinal microecological balance	1673:1712	It could be concluded that the addition of 6% fermented maize cob feed to the diets of finishing pigs could promote their growth, improve their production performance and slaughter performance meat quality, and enhance their intestinal microecological balance and immunity.
33274228	8	43	theme	%	1498:1498	arg1	feed					1520:1523	6% fermented maize cob feed	1497:1523	6% fermented maize cob feed	1497:1523	It could be concluded that the addition of 6% fermented maize cob feed to the diets of finishing pigs could promote their growth, improve their production performance and slaughter performance meat quality, and enhance their intestinal microecological balance and immunity.
33274228	1	44	theme	composite	236:244	arg1	probiotics					246:255	composite probiotics	236:255	2.5% composite probiotics	231:255	1000 g maize cob mixed material was synergistically fermented by adding 2.5% composite probiotics and 0.06-0.08% NSP (nonstarch polysaccharide) enzyme to prepare fermented feed, and its effectiveness as feed for fattening pigs was investigated.
33274228	5	45	theme	finishing	1035:1043	arg1	pigs					1045:1048	the finishing pigs	1031:1048	the finishing pigs	1031:1048	In addition, beneficial bacteria including Lactobacillus in the intestines of the finishing pigs significantly increased, and pathogenic bacteria including Escherichia coli in the intestines and feces were found to be significantly reduced (p < 0.05).
33274228	2	46	theme	×	568:568	arg1	number					541:546	the total number	531:546	the total number of bacteria	531:558	The results showed that the appearance, texture, and nutrient quality of maize cobs significantly improved after fermentation, the total number of bacteria was 4.5 × 1010 CFU/g, and the protein content was 7.1%.
33274228	2	46	theme	×	568:568	arg1	1010 CFU/g					570:579	4.5 × 1010 CFU/g	564:579	4.5 × 1010 CFU/g	564:579	The results showed that the appearance, texture, and nutrient quality of maize cobs significantly improved after fermentation, the total number of bacteria was 4.5 × 1010 CFU/g, and the protein content was 7.1%.
33274228	8	47	theme	maize	1510:1514	arg1	feed					1520:1523	6% fermented maize cob feed	1497:1523	6% fermented maize cob feed	1497:1523	It could be concluded that the addition of 6% fermented maize cob feed to the diets of finishing pigs could promote their growth, improve their production performance and slaughter performance meat quality, and enhance their intestinal microecological balance and immunity.
33274228	3	48	theme	6	663:663	arg1	%					664:664	%	664:664	%	664:664	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	8	49	theme	feed	1520:1523	arg1	addition					1485:1492	the addition	1481:1492	the addition of 6% fermented maize cob feed to the diets of finishing pigs	1481:1554	It could be concluded that the addition of 6% fermented maize cob feed to the diets of finishing pigs could promote their growth, improve their production performance and slaughter performance meat quality, and enhance their intestinal microecological balance and immunity.
33274228	1	50	theme	1000 g	159:164	arg1	material					182:189	1000 g maize cob mixed material	159:189	1000 g maize cob mixed material	159:189	1000 g maize cob mixed material was synergistically fermented by adding 2.5% composite probiotics and 0.06-0.08% NSP (nonstarch polysaccharide) enzyme to prepare fermented feed, and its effectiveness as feed for fattening pigs was investigated.
33274228	6	51	theme	pigs	1290:1293	arg1	immunity					1264:1271	ileal mucosal immunity	1250:1271	ileal mucosal immunity	1250:1271	The intestinal crypt depth, VH/CD ratio, and ileal mucosal immunity of the finishing pigs also significantly improved (p < 0.05).
33274228	6	51	theme	pigs	1290:1293	arg1	depth					1226:1230	The intestinal crypt depth	1205:1230	The intestinal crypt depth	1205:1230	The intestinal crypt depth, VH/CD ratio, and ileal mucosal immunity of the finishing pigs also significantly improved (p < 0.05).
33274228	6	51	theme	pigs	1290:1293	arg1	ratio					1239:1243	VH/CD ratio	1233:1243	VH/CD ratio	1233:1243	The intestinal crypt depth, VH/CD ratio, and ileal mucosal immunity of the finishing pigs also significantly improved (p < 0.05).
33274228	5	52	dep	reduced	1185:1191	arg1	<					1196:1196	p < 0.05	1194:1201	p < 0.05	1194:1201	In addition, beneficial bacteria including Lactobacillus in the intestines of the finishing pigs significantly increased, and pathogenic bacteria including Escherichia coli in the intestines and feces were found to be significantly reduced (p < 0.05).
33274228	3	53	from	pigs	651:654	arg1	group					704:708	the 6% fermented maize cob feed experimental group	659:708	the 6% fermented maize cob feed experimental group	659:708	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	5	54	from	bacteria	1090:1097	arg1	intestines					1133:1142	intestines	1133:1142	intestines	1133:1142	In addition, beneficial bacteria including Lactobacillus in the intestines of the finishing pigs significantly increased, and pathogenic bacteria including Escherichia coli in the intestines and feces were found to be significantly reduced (p < 0.05).
33274228	5	54	from	bacteria	1090:1097	arg1	feces					1148:1152	feces	1148:1152	feces	1148:1152	In addition, beneficial bacteria including Lactobacillus in the intestines of the finishing pigs significantly increased, and pathogenic bacteria including Escherichia coli in the intestines and feces were found to be significantly reduced (p < 0.05).
33274228	1	55	theme	cob	172:174	arg1	material					182:189	1000 g maize cob mixed material	159:189	1000 g maize cob mixed material	159:189	1000 g maize cob mixed material was synergistically fermented by adding 2.5% composite probiotics and 0.06-0.08% NSP (nonstarch polysaccharide) enzyme to prepare fermented feed, and its effectiveness as feed for fattening pigs was investigated.
33274228	1	56	dep	%	270:270	arg1	enzyme					303:308	NSP (nonstarch polysaccharide) enzyme	272:308	0.06-0.08% NSP (nonstarch polysaccharide) enzyme	261:308	1000 g maize cob mixed material was synergistically fermented by adding 2.5% composite probiotics and 0.06-0.08% NSP (nonstarch polysaccharide) enzyme to prepare fermented feed, and its effectiveness as feed for fattening pigs was investigated.
33274228	3	57	theme	cob	682:684	arg1	group					704:708	the 6% fermented maize cob feed experimental group	659:708	the 6% fermented maize cob feed experimental group	659:708	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	3	58	theme	fermented	666:674	arg1	group					704:708	the 6% fermented maize cob feed experimental group	659:708	the 6% fermented maize cob feed experimental group	659:708	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	8	59	theme	pigs	1551:1554	arg1	addition					1485:1492	the addition	1481:1492	the addition of 6% fermented maize cob feed to the diets of finishing pigs	1481:1554	It could be concluded that the addition of 6% fermented maize cob feed to the diets of finishing pigs could promote their growth, improve their production performance and slaughter performance meat quality, and enhance their intestinal microecological balance and immunity.
33274228	6	60	theme	mucosal	1256:1262	arg1	immunity					1264:1271	ileal mucosal immunity	1250:1271	ileal mucosal immunity	1250:1271	The intestinal crypt depth, VH/CD ratio, and ileal mucosal immunity of the finishing pigs also significantly improved (p < 0.05).
33274228	6	61	theme	crypt	1220:1224	arg1	depth					1226:1230	The intestinal crypt depth	1205:1230	The intestinal crypt depth	1205:1230	The intestinal crypt depth, VH/CD ratio, and ileal mucosal immunity of the finishing pigs also significantly improved (p < 0.05).
33274228	5	62	dep	intestines	1133:1142	arg1	the					1129:1131	the	1129:1131	the	1129:1131	In addition, beneficial bacteria including Lactobacillus in the intestines of the finishing pigs significantly increased, and pathogenic bacteria including Escherichia coli in the intestines and feces were found to be significantly reduced (p < 0.05).
33274228	5	63	theme	p	1194:1194	arg1	<					1196:1196	p < 0.05	1194:1201	p < 0.05	1194:1201	In addition, beneficial bacteria including Lactobacillus in the intestines of the finishing pigs significantly increased, and pathogenic bacteria including Escherichia coli in the intestines and feces were found to be significantly reduced (p < 0.05).
33274228	3	64	theme	experimental	691:702	arg1	group					704:708	the 6% fermented maize cob feed experimental group	659:708	the 6% fermented maize cob feed experimental group	659:708	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	3	65	theme	nutrient	783:790	arg1	rate					802:805	nutrient digestion rate	783:805	nutrient digestion rate (p < 0.05)	783:816	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	3	65	theme	nutrient	783:790	arg1	<					810:810	p < 0.05	808:815	p < 0.05	808:815	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	7	66	theme	p	1443:1443	arg1	<					1445:1445	p < 0.05	1443:1450	p < 0.05	1443:1450	The cytokine content and gene expression of sIgA, IL-8, and TNF-α were found to be significantly increased (p < 0.05).
33274228	2	67	theme	total	535:539	arg1	number					541:546	the total number	531:546	the total number of bacteria	531:558	The results showed that the appearance, texture, and nutrient quality of maize cobs significantly improved after fermentation, the total number of bacteria was 4.5 × 1010 CFU/g, and the protein content was 7.1%.
33274228	2	67	theme	total	535:539	arg1	1010 CFU/g					570:579	4.5 × 1010 CFU/g	564:579	4.5 × 1010 CFU/g	564:579	The results showed that the appearance, texture, and nutrient quality of maize cobs significantly improved after fermentation, the total number of bacteria was 4.5 × 1010 CFU/g, and the protein content was 7.1%.
33274228	3	68	theme	feed	747:750	arg1	intake					752:757	daily feed intake	741:757	daily feed intake	741:757	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	6	69	theme	finishing	1280:1288	arg1	pigs					1290:1293	the finishing pigs	1276:1293	the finishing pigs	1276:1293	The intestinal crypt depth, VH/CD ratio, and ileal mucosal immunity of the finishing pigs also significantly improved (p < 0.05).
33274228	6	70	theme	ileal	1250:1254	arg1	immunity					1264:1271	ileal mucosal immunity	1250:1271	ileal mucosal immunity	1250:1271	The intestinal crypt depth, VH/CD ratio, and ileal mucosal immunity of the finishing pigs also significantly improved (p < 0.05).
33274228	0	71	theme	Synergistic	76:86	arg1	Feed					108:111	Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed	30:111	Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed	30:111	A Study on the Preparation of Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed and Its Feeding Efficiency in Finishing Pigs.
33274228	1	72	theme	fermented	321:329	arg1	feed					331:334	fermented feed	321:334	fermented feed	321:334	1000 g maize cob mixed material was synergistically fermented by adding 2.5% composite probiotics and 0.06-0.08% NSP (nonstarch polysaccharide) enzyme to prepare fermented feed, and its effectiveness as feed for fattening pigs was investigated.
33274228	8	73	theme	production	1598:1607	arg1	performance					1609:1619	their production performance and slaughter performance meat quality	1592:1658	performance	1609:1619	It could be concluded that the addition of 6% fermented maize cob feed to the diets of finishing pigs could promote their growth, improve their production performance and slaughter performance meat quality, and enhance their intestinal microecological balance and immunity.
33274228	3	74	theme	p	808:808	arg1	rate					802:805	nutrient digestion rate	783:805	nutrient digestion rate (p < 0.05)	783:816	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	3	74	theme	p	808:808	arg1	<					810:810	p < 0.05	808:815	p < 0.05	808:815	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	4	75	theme	Most	864:867	arg1	performance					900:910	slaughter performance	890:910	slaughter performance	890:910	Most indicators including slaughter performance and meat quality significantly improved.
33274228	4	75	theme	Most	864:867	arg1	quality					921:927	meat quality	916:927	meat quality	916:927	Most indicators including slaughter performance and meat quality significantly improved.
33274228	4	75	theme	Most	864:867	arg1	indicators					869:878	Most indicators	864:878	Most indicators including slaughter performance and meat quality	864:927	Most indicators including slaughter performance and meat quality significantly improved.
33274228	0	76	from	Study	2:6	arg1	Efficiency					129:138	Its Feeding Efficiency	117:138	Its Feeding Efficiency in Finishing Pigs	117:156	A Study on the Preparation of Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed and Its Feeding Efficiency in Finishing Pigs.
33274228	0	76	from	Study	2:6	arg1	Preparation					15:25	the Preparation	11:25	the Preparation of Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed	11:111	A Study on the Preparation of Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed and Its Feeding Efficiency in Finishing Pigs.
33274228	3	77	theme	weight	766:771	arg1	gain					773:776	daily weight gain	760:776	daily weight gain	760:776	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	0	78	theme	Maize	98:102	arg1	Feed					108:111	Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed	30:111	Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed	30:111	A Study on the Preparation of Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed and Its Feeding Efficiency in Finishing Pigs.
33274228	0	79	theme	Feed	108:111	arg1	Efficiency					129:138	Its Feeding Efficiency	117:138	Its Feeding Efficiency in Finishing Pigs	117:156	A Study on the Preparation of Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed and Its Feeding Efficiency in Finishing Pigs.
33274228	0	79	theme	Feed	108:111	arg1	Preparation					15:25	the Preparation	11:25	the Preparation of Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed	11:111	A Study on the Preparation of Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed and Its Feeding Efficiency in Finishing Pigs.
33274228	8	80	theme	performance	1635:1645	arg1	quality					1652:1658	their production performance and slaughter performance meat quality	1592:1658	quality	1652:1658	It could be concluded that the addition of 6% fermented maize cob feed to the diets of finishing pigs could promote their growth, improve their production performance and slaughter performance meat quality, and enhance their intestinal microecological balance and immunity.
33274228	2	81	theme	maize	477:481	arg1	cobs					483:486	maize cobs	477:486	maize cobs	477:486	The results showed that the appearance, texture, and nutrient quality of maize cobs significantly improved after fermentation, the total number of bacteria was 4.5 × 1010 CFU/g, and the protein content was 7.1%.
33274228	3	82	dep	showed	710:715	arg1	increased					731:739	increased	731:739	increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05)	731:816	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	3	82	dep	showed	710:715	arg1	reduced					822:828	reduced	822:828	reduced feed conversion ratio (p < 0.05)	822:861	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	4	83	theme	meat	916:919	arg1	quality					921:927	meat quality	916:927	meat quality	916:927	Most indicators including slaughter performance and meat quality significantly improved.
33274228	6	84	theme	p	1324:1324	arg1	<					1326:1326	p < 0.05	1324:1331	p < 0.05	1324:1331	The intestinal crypt depth, VH/CD ratio, and ileal mucosal immunity of the finishing pigs also significantly improved (p < 0.05).
33274228	1	85	theme	NSP	272:274	arg1	enzyme					303:308	NSP (nonstarch polysaccharide) enzyme	272:308	0.06-0.08% NSP (nonstarch polysaccharide) enzyme	261:308	1000 g maize cob mixed material was synergistically fermented by adding 2.5% composite probiotics and 0.06-0.08% NSP (nonstarch polysaccharide) enzyme to prepare fermented feed, and its effectiveness as feed for fattening pigs was investigated.
33274228	1	86	theme	nonstarch	277:285	arg1	enzyme					303:308	NSP (nonstarch polysaccharide) enzyme	272:308	0.06-0.08% NSP (nonstarch polysaccharide) enzyme	261:308	1000 g maize cob mixed material was synergistically fermented by adding 2.5% composite probiotics and 0.06-0.08% NSP (nonstarch polysaccharide) enzyme to prepare fermented feed, and its effectiveness as feed for fattening pigs was investigated.
33274228	6	87	dep	improved	1314:1321	arg1	<					1326:1326	p < 0.05	1324:1331	p < 0.05	1324:1331	The intestinal crypt depth, VH/CD ratio, and ileal mucosal immunity of the finishing pigs also significantly improved (p < 0.05).
33274228	3	88	theme	feed	830:833	arg1	<					855:855	p < 0.05	853:860	p < 0.05	853:860	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	3	88	theme	feed	830:833	arg1	ratio					846:850	feed conversion ratio	830:850	feed conversion ratio (p < 0.05)	830:861	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	5	89	from	bacteria	977:984	arg1	intestines					1017:1026	the intestines	1013:1026	the intestines of the finishing pigs	1013:1048	In addition, beneficial bacteria including Lactobacillus in the intestines of the finishing pigs significantly increased, and pathogenic bacteria including Escherichia coli in the intestines and feces were found to be significantly reduced (p < 0.05).
33274228	5	90	theme	pathogenic	1079:1088	arg1	bacteria					1090:1097	pathogenic bacteria	1079:1097	pathogenic bacteria including Escherichia coli in the intestines and feces	1079:1152	In addition, beneficial bacteria including Lactobacillus in the intestines of the finishing pigs significantly increased, and pathogenic bacteria including Escherichia coli in the intestines and feces were found to be significantly reduced (p < 0.05).
33274228	5	90	theme	pathogenic	1079:1088	arg1	coli					1121:1124	Escherichia coli	1109:1124	Escherichia coli	1109:1124	In addition, beneficial bacteria including Lactobacillus in the intestines of the finishing pigs significantly increased, and pathogenic bacteria including Escherichia coli in the intestines and feces were found to be significantly reduced (p < 0.05).
33274228	7	91	theme	IL-8	1385:1388	arg1	content					1348:1354	cytokine content	1339:1354	cytokine content	1339:1354	The cytokine content and gene expression of sIgA, IL-8, and TNF-α were found to be significantly increased (p < 0.05).
33274228	7	91	theme	IL-8	1385:1388	arg1	expression					1365:1374	gene expression	1360:1374	gene expression	1360:1374	The cytokine content and gene expression of sIgA, IL-8, and TNF-α were found to be significantly increased (p < 0.05).
33274228	3	92	theme	p	853:853	arg1	<					855:855	p < 0.05	853:860	p < 0.05	853:860	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	3	92	theme	p	853:853	arg1	ratio					846:850	feed conversion ratio	830:850	feed conversion ratio (p < 0.05)	830:861	Compared to the control group, the pigs in the 6% fermented maize cob feed experimental group showed significantly increased daily feed intake, daily weight gain, and nutrient digestion rate (p < 0.05) and reduced feed conversion ratio (p < 0.05).
33274228	0	93	from	Efficiency	129:138	arg1	Pigs					153:156	Finishing Pigs	143:156	Finishing Pigs	143:156	A Study on the Preparation of Microbial and Nonstarch Polysaccharide Enzyme Synergistic Fermented Maize Cob Feed and Its Feeding Efficiency in Finishing Pigs.
33274228	8	94	theme	6	1497:1497	arg1	%					1498:1498	%	1498:1498	%	1498:1498	It could be concluded that the addition of 6% fermented maize cob feed to the diets of finishing pigs could promote their growth, improve their production performance and slaughter performance meat quality, and enhance their intestinal microecological balance and immunity.
33274228	7	95	theme	sIgA	1379:1382	arg1	content					1348:1354	cytokine content	1339:1354	cytokine content	1339:1354	The cytokine content and gene expression of sIgA, IL-8, and TNF-α were found to be significantly increased (p < 0.05).
33274228	7	95	theme	sIgA	1379:1382	arg1	expression					1365:1374	gene expression	1360:1374	gene expression	1360:1374	The cytokine content and gene expression of sIgA, IL-8, and TNF-α were found to be significantly increased (p < 0.05).
33274228	8	96	theme	fermented	1500:1508	arg1	feed					1520:1523	6% fermented maize cob feed	1497:1523	6% fermented maize cob feed	1497:1523	It could be concluded that the addition of 6% fermented maize cob feed to the diets of finishing pigs could promote their growth, improve their production performance and slaughter performance meat quality, and enhance their intestinal microecological balance and immunity.
32842418	3	0	theme	wound	477:481	arg1	dressings					483:491	wound dressings	477:491	wound dressings	477:491	In this paper, the characteristics of chitosan nanofibers as wound dressings and the newly developed chitosan nanofiber composite materials are introduced.
32842418	2	1	theme	other	320:324	arg1	materials					334:342	other medical materials	320:342	other medical materials	320:342	One of the research directions in this field is to composite and modificate chitosan with other medical materials to prepare nanofiber composite materials and to study their properties.
32842418	0	2	from	[Advances	0:8	arg1	research					17:24	the research	13:24	the research of wound dressings	13:43	[Advances in the research of wound dressings based on chitosan nanofibers].
32842418	3	3	theme	chitosan	517:524	arg1	materials					546:554	the newly developed chitosan nanofiber composite materials	497:554	the newly developed chitosan nanofiber composite materials	497:554	In this paper, the characteristics of chitosan nanofibers as wound dressings and the newly developed chitosan nanofiber composite materials are introduced.
32842418	2	4	with	composite	281:289	arg1	materials					334:342	other medical materials	320:342	other medical materials	320:342	One of the research directions in this field is to composite and modificate chitosan with other medical materials to prepare nanofiber composite materials and to study their properties.
32842418	3	5	theme	nanofiber	526:534	arg1	materials					546:554	the newly developed chitosan nanofiber composite materials	497:554	the newly developed chitosan nanofiber composite materials	497:554	In this paper, the characteristics of chitosan nanofibers as wound dressings and the newly developed chitosan nanofiber composite materials are introduced.
32842418	1	6	theme	wound	128:132	arg1	dressings					134:142	wound dressings	128:142	wound dressings	128:142	Chitosan nanofibers are widely used in the field of wound dressings due to their excellent biocompatibility, antibacterial activity, and healing ability.
32842418	2	7	theme	directions	250:259	arg1	One					230:232	One	230:232	One	230:232	One of the research directions in this field is to composite and modificate chitosan with other medical materials to prepare nanofiber composite materials and to study their properties.
32842418	2	7	theme	directions	250:259	arg1	directions					250:259	the research directions	237:259	the research directions in this field	237:273	One of the research directions in this field is to composite and modificate chitosan with other medical materials to prepare nanofiber composite materials and to study their properties.
32842418	2	8	theme	research	241:248	arg1	directions					250:259	the research directions	237:259	the research directions in this field	237:273	One of the research directions in this field is to composite and modificate chitosan with other medical materials to prepare nanofiber composite materials and to study their properties.
32842418	2	9	theme	composite	365:373	arg1	materials					375:383	nanofiber composite materials	355:383	nanofiber composite materials	355:383	One of the research directions in this field is to composite and modificate chitosan with other medical materials to prepare nanofiber composite materials and to study their properties.
32842418	2	10	theme	nanofiber	355:363	arg1	materials					375:383	nanofiber composite materials	355:383	nanofiber composite materials	355:383	One of the research directions in this field is to composite and modificate chitosan with other medical materials to prepare nanofiber composite materials and to study their properties.
32842418	1	11	theme	Chitosan	76:83	arg1	nanofibers					85:94	Chitosan nanofibers	76:94	Chitosan nanofibers	76:94	Chitosan nanofibers are widely used in the field of wound dressings due to their excellent biocompatibility, antibacterial activity, and healing ability.
32842418	1	12	theme	excellent	157:165	arg1	biocompatibility					167:182	their excellent biocompatibility	151:182	their excellent biocompatibility	151:182	Chitosan nanofibers are widely used in the field of wound dressings due to their excellent biocompatibility, antibacterial activity, and healing ability.
32842418	1	13	used	used	107:110	arg2	nanofibers					85:94	Chitosan nanofibers	76:94	Chitosan nanofibers	76:94	Chitosan nanofibers are widely used in the field of wound dressings due to their excellent biocompatibility, antibacterial activity, and healing ability.
32842418	1	14	theme	dressings	134:142	arg1	field					119:123	the field	115:123	the field of wound dressings due to their excellent biocompatibility, antibacterial activity, and healing ability	115:227	Chitosan nanofibers are widely used in the field of wound dressings due to their excellent biocompatibility, antibacterial activity, and healing ability.
32842418	0	15	theme	wound	29:33	arg1	dressings					35:43	wound dressings	29:43	wound dressings	29:43	[Advances in the research of wound dressings based on chitosan nanofibers].
32842418	1	16	theme	antibacterial	185:197	arg1	activity					199:206	antibacterial activity	185:206	antibacterial activity	185:206	Chitosan nanofibers are widely used in the field of wound dressings due to their excellent biocompatibility, antibacterial activity, and healing ability.
32842418	2	17	with	chitosan	306:313	arg1	materials					334:342	other medical materials	320:342	other medical materials	320:342	One of the research directions in this field is to composite and modificate chitosan with other medical materials to prepare nanofiber composite materials and to study their properties.
32842418	3	18	theme	nanofibers	463:472	arg1	characteristics					435:449	the characteristics	431:449	the characteristics of chitosan nanofibers as wound dressings and the newly developed chitosan nanofiber composite materials	431:554	In this paper, the characteristics of chitosan nanofibers as wound dressings and the newly developed chitosan nanofiber composite materials are introduced.
32842418	0	19	theme	dressings	35:43	arg1	research					17:24	the research	13:24	the research of wound dressings	13:43	[Advances in the research of wound dressings based on chitosan nanofibers].
32842418	2	20	from	directions	250:259	arg1	field					269:273	this field	264:273	this field	264:273	One of the research directions in this field is to composite and modificate chitosan with other medical materials to prepare nanofiber composite materials and to study their properties.
32842418	2	21	theme	modificate	295:304	arg1	chitosan					306:313	modificate chitosan	295:313	modificate chitosan	295:313	One of the research directions in this field is to composite and modificate chitosan with other medical materials to prepare nanofiber composite materials and to study their properties.
32842418	3	22	theme	composite	536:544	arg1	materials					546:554	the newly developed chitosan nanofiber composite materials	497:554	the newly developed chitosan nanofiber composite materials	497:554	In this paper, the characteristics of chitosan nanofibers as wound dressings and the newly developed chitosan nanofiber composite materials are introduced.
32842418	3	23	theme	developed	507:515	arg1	materials					546:554	the newly developed chitosan nanofiber composite materials	497:554	the newly developed chitosan nanofiber composite materials	497:554	In this paper, the characteristics of chitosan nanofibers as wound dressings and the newly developed chitosan nanofiber composite materials are introduced.
32842418	3	24	theme	chitosan	454:461	arg1	nanofibers					463:472	chitosan nanofibers	454:472	chitosan nanofibers as wound dressings and the newly developed chitosan nanofiber composite materials	454:554	In this paper, the characteristics of chitosan nanofibers as wound dressings and the newly developed chitosan nanofiber composite materials are introduced.
32842418	0	25	theme	chitosan	54:61	arg1	nanofibers					63:72	chitosan nanofibers	54:72	chitosan nanofibers	54:72	[Advances in the research of wound dressings based on chitosan nanofibers].
32842418	2	26	theme	medical	326:332	arg1	materials					334:342	other medical materials	320:342	other medical materials	320:342	One of the research directions in this field is to composite and modificate chitosan with other medical materials to prepare nanofiber composite materials and to study their properties.
32842418	1	27	theme	healing	213:219	arg1	ability					221:227	healing ability	213:227	healing ability	213:227	Chitosan nanofibers are widely used in the field of wound dressings due to their excellent biocompatibility, antibacterial activity, and healing ability.
34528649	4	0	from	TNF-α	885:889	arg1	mice					910:913	mice	910:913	mice	910:913	JSP modulated oxidative stress and inflammatory responses, which was demonstrated by reduced MPO activity, NO level, and levels of pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 in mice.
34528649	4	1	theme	reduced	802:808	arg1	activity					814:821	reduced MPO activity	802:821	reduced MPO activity	802:821	JSP modulated oxidative stress and inflammatory responses, which was demonstrated by reduced MPO activity, NO level, and levels of pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 in mice.
34528649	0	2	theme	gut	84:86	arg1	microbiota					88:97	the gut microbiota	80:97	the gut microbiota in mice with DSS-induced colitis	80:130	Jellyfish skin polysaccharides enhance intestinal barrier function and modulate the gut microbiota in mice with DSS-induced colitis.
34528649	3	3	theme	epithelial	678:687	arg1	integrity					689:697	intestinal epithelial integrity	667:697	intestinal epithelial integrity	667:697	JSP supplementation reduced the symptoms of colitis in mice, increased colon length, protected goblet cells, and improved intestinal epithelial integrity and permeability.
34528649	4	4	theme	cytokines	865:873	arg1	activity					814:821	reduced MPO activity	802:821	reduced MPO activity	802:821	JSP modulated oxidative stress and inflammatory responses, which was demonstrated by reduced MPO activity, NO level, and levels of pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 in mice.
34528649	4	4	theme	cytokines	865:873	arg1	level					827:831	NO level	824:831	NO level	824:831	JSP modulated oxidative stress and inflammatory responses, which was demonstrated by reduced MPO activity, NO level, and levels of pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 in mice.
34528649	4	4	theme	cytokines	865:873	arg1	levels					838:843	levels	838:843	levels of pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 in mice	838:913	JSP modulated oxidative stress and inflammatory responses, which was demonstrated by reduced MPO activity, NO level, and levels of pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 in mice.
34528649	6	5	theme	proteins	1088:1095	arg1	expression					1059:1068	the expression	1055:1068	the expression of tight junction proteins and mucins	1055:1106	Moreover, JSP supplementation enhanced the expression of tight junction proteins and mucins, and modulated the composition of the gut microbiota and the production of short-chain fatty acids.
34528649	0	6	from	microbiota	88:97	arg1	mice					102:105	mice	102:105	mice with DSS-induced colitis	102:130	Jellyfish skin polysaccharides enhance intestinal barrier function and modulate the gut microbiota in mice with DSS-induced colitis.
34528649	6	7	theme	acids	1201:1205	arg1	production					1169:1178	the production	1165:1178	the production of short-chain fatty acids	1165:1205	Moreover, JSP supplementation enhanced the expression of tight junction proteins and mucins, and modulated the composition of the gut microbiota and the production of short-chain fatty acids.
34528649	6	7	theme	acids	1201:1205	arg1	composition					1127:1137	the composition	1123:1137	the composition of the gut microbiota	1123:1159	Moreover, JSP supplementation enhanced the expression of tight junction proteins and mucins, and modulated the composition of the gut microbiota and the production of short-chain fatty acids.
34528649	3	8	theme	goblet	640:645	arg1	cells					647:651	goblet cells	640:651	goblet cells	640:651	JSP supplementation reduced the symptoms of colitis in mice, increased colon length, protected goblet cells, and improved intestinal epithelial integrity and permeability.
34528649	4	9	theme	inflammatory	752:763	arg1	responses					765:773	inflammatory responses	752:773	inflammatory responses	752:773	JSP modulated oxidative stress and inflammatory responses, which was demonstrated by reduced MPO activity, NO level, and levels of pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 in mice.
34528649	1	10	theme	uronic	268:273	arg1	acid					275:278	uronic acid	268:278	2.26% uronic acid	262:278	Jellyfish skin polysaccharides (JSP) were isolated from Rhopilema esculentum Kishinouye and contained 55.11% polysaccharides and 2.26% uronic acid.
34528649	6	11	theme	microbiota	1150:1159	arg1	production					1169:1178	the production	1165:1178	the production of short-chain fatty acids	1165:1205	Moreover, JSP supplementation enhanced the expression of tight junction proteins and mucins, and modulated the composition of the gut microbiota and the production of short-chain fatty acids.
34528649	6	11	theme	microbiota	1150:1159	arg1	composition					1127:1137	the composition	1123:1137	the composition of the gut microbiota	1123:1159	Moreover, JSP supplementation enhanced the expression of tight junction proteins and mucins, and modulated the composition of the gut microbiota and the production of short-chain fatty acids.
34528649	4	12	from	IL-6	902:905	arg1	mice					910:913	mice	910:913	mice	910:913	JSP modulated oxidative stress and inflammatory responses, which was demonstrated by reduced MPO activity, NO level, and levels of pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 in mice.
34528649	3	13	theme	JSP	545:547	arg1	supplementation					549:563	JSP supplementation	545:563	JSP supplementation	545:563	JSP supplementation reduced the symptoms of colitis in mice, increased colon length, protected goblet cells, and improved intestinal epithelial integrity and permeability.
34528649	2	14	theme	immunomodulatory	331:346	arg1	activities					348:357	the anti-inflammatory, antioxidant and immunomodulatory activities	292:357	the anti-inflammatory, antioxidant and immunomodulatory activities of JSP	292:364	To examine the anti-inflammatory, antioxidant and immunomodulatory activities of JSP in vivo, C57BL/6 mice were induced to develop ulcerative colitis by dextran sulfate sodium (DSS) and the roles of dietary JSP supplementation in modulating colitis were explored.
34528649	0	15	theme	DSS-induced	112:122	arg1	colitis					124:130	DSS-induced colitis	112:130	DSS-induced colitis	112:130	Jellyfish skin polysaccharides enhance intestinal barrier function and modulate the gut microbiota in mice with DSS-induced colitis.
34528649	7	16	theme	dietary	1277:1283	arg1	JSP					1285:1287	dietary JSP	1277:1287	dietary JSP	1277:1287	Taken together, these results reveal the anti-inflammatory effect of dietary JSP in vivo, suggesting the potential of JSP as a nutritional supplement or adjunct strategy in preventing or ameliorating colitis.
34528649	5	17	theme	lower	972:976	arg1	levels					978:983	lower levels	972:983	lower levels of phosphorylated p65 and IKB	972:1013	JSP suppressed NF-κB signaling pathways as evidenced by lower levels of phosphorylated p65 and IKB.
34528649	6	18	theme	junction	1079:1086	arg1	proteins					1088:1095	tight junction proteins	1073:1095	tight junction proteins	1073:1095	Moreover, JSP supplementation enhanced the expression of tight junction proteins and mucins, and modulated the composition of the gut microbiota and the production of short-chain fatty acids.
34528649	2	19	theme	supplementation	492:506	arg1	roles					471:475	the roles	467:475	the roles of dietary JSP supplementation in modulating colitis	467:528	To examine the anti-inflammatory, antioxidant and immunomodulatory activities of JSP in vivo, C57BL/6 mice were induced to develop ulcerative colitis by dextran sulfate sodium (DSS) and the roles of dietary JSP supplementation in modulating colitis were explored.
34528649	7	20	theme	supplement	1347:1356	arg1	strategy					1369:1376	a nutritional supplement or adjunct strategy	1333:1376	a nutritional supplement or adjunct strategy in preventing or ameliorating colitis	1333:1414	Taken together, these results reveal the anti-inflammatory effect of dietary JSP in vivo, suggesting the potential of JSP as a nutritional supplement or adjunct strategy in preventing or ameliorating colitis.
34528649	6	21	theme	tight	1073:1077	arg1	proteins					1088:1095	tight junction proteins	1073:1095	tight junction proteins	1073:1095	Moreover, JSP supplementation enhanced the expression of tight junction proteins and mucins, and modulated the composition of the gut microbiota and the production of short-chain fatty acids.
34528649	0	22	theme	skin	10:13	arg1	polysaccharides					15:29	Jellyfish skin polysaccharides	0:29	Jellyfish skin polysaccharides	0:29	Jellyfish skin polysaccharides enhance intestinal barrier function and modulate the gut microbiota in mice with DSS-induced colitis.
34528649	4	23	theme	pro-inflammatory	848:863	arg1	IL-6					902:905	IL-6	902:905	IL-6	902:905	JSP modulated oxidative stress and inflammatory responses, which was demonstrated by reduced MPO activity, NO level, and levels of pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 in mice.
34528649	4	23	theme	pro-inflammatory	848:863	arg1	IL-1β					892:896	IL-1β	892:896	IL-1β	892:896	JSP modulated oxidative stress and inflammatory responses, which was demonstrated by reduced MPO activity, NO level, and levels of pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 in mice.
34528649	4	23	theme	pro-inflammatory	848:863	arg1	TNF-α					885:889	TNF-α	885:889	TNF-α	885:889	JSP modulated oxidative stress and inflammatory responses, which was demonstrated by reduced MPO activity, NO level, and levels of pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 in mice.
34528649	4	23	theme	pro-inflammatory	848:863	arg1	cytokines					865:873	pro-inflammatory cytokines	848:873	pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 in mice	848:913	JSP modulated oxidative stress and inflammatory responses, which was demonstrated by reduced MPO activity, NO level, and levels of pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 in mice.
34528649	7	24	theme	anti-inflammatory	1249:1265	arg1	effect					1267:1272	the anti-inflammatory effect	1245:1272	the anti-inflammatory effect of dietary JSP	1245:1287	Taken together, these results reveal the anti-inflammatory effect of dietary JSP in vivo, suggesting the potential of JSP as a nutritional supplement or adjunct strategy in preventing or ameliorating colitis.
34528649	5	25	theme	NF-κB	931:935	arg1	pathways					947:954	NF-κB signaling pathways	931:954	NF-κB signaling pathways	931:954	JSP suppressed NF-κB signaling pathways as evidenced by lower levels of phosphorylated p65 and IKB.
34528649	0	26	theme	Jellyfish	0:8	arg1	polysaccharides					15:29	Jellyfish skin polysaccharides	0:29	Jellyfish skin polysaccharides	0:29	Jellyfish skin polysaccharides enhance intestinal barrier function and modulate the gut microbiota in mice with DSS-induced colitis.
34528649	4	27	from	IL-1β	892:896	arg1	mice					910:913	mice	910:913	mice	910:913	JSP modulated oxidative stress and inflammatory responses, which was demonstrated by reduced MPO activity, NO level, and levels of pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 in mice.
34528649	0	28	with	mice	102:105	arg1	colitis					124:130	DSS-induced colitis	112:130	DSS-induced colitis	112:130	Jellyfish skin polysaccharides enhance intestinal barrier function and modulate the gut microbiota in mice with DSS-induced colitis.
34528649	2	29	theme	antioxidant	315:325	arg1	activities					348:357	the anti-inflammatory, antioxidant and immunomodulatory activities	292:357	the anti-inflammatory, antioxidant and immunomodulatory activities of JSP	292:364	To examine the anti-inflammatory, antioxidant and immunomodulatory activities of JSP in vivo, C57BL/6 mice were induced to develop ulcerative colitis by dextran sulfate sodium (DSS) and the roles of dietary JSP supplementation in modulating colitis were explored.
34528649	2	30	theme	sulfate	442:448	arg1	sodium					450:455	dextran sulfate sodium	434:455	dextran sulfate sodium (DSS)	434:461	To examine the anti-inflammatory, antioxidant and immunomodulatory activities of JSP in vivo, C57BL/6 mice were induced to develop ulcerative colitis by dextran sulfate sodium (DSS) and the roles of dietary JSP supplementation in modulating colitis were explored.
34528649	2	30	theme	sulfate	442:448	arg1	DSS					458:460	DSS	458:460	DSS	458:460	To examine the anti-inflammatory, antioxidant and immunomodulatory activities of JSP in vivo, C57BL/6 mice were induced to develop ulcerative colitis by dextran sulfate sodium (DSS) and the roles of dietary JSP supplementation in modulating colitis were explored.
34528649	6	31	theme	fatty	1195:1199	arg1	acids					1201:1205	short-chain fatty acids	1183:1205	short-chain fatty acids	1183:1205	Moreover, JSP supplementation enhanced the expression of tight junction proteins and mucins, and modulated the composition of the gut microbiota and the production of short-chain fatty acids.
34528649	7	32	theme	adjunct	1361:1367	arg1	strategy					1369:1376	a nutritional supplement or adjunct strategy	1333:1376	a nutritional supplement or adjunct strategy in preventing or ameliorating colitis	1333:1414	Taken together, these results reveal the anti-inflammatory effect of dietary JSP in vivo, suggesting the potential of JSP as a nutritional supplement or adjunct strategy in preventing or ameliorating colitis.
34528649	5	33	theme	IKB	1011:1013	arg1	levels					978:983	lower levels	972:983	lower levels of phosphorylated p65 and IKB	972:1013	JSP suppressed NF-κB signaling pathways as evidenced by lower levels of phosphorylated p65 and IKB.
34528649	2	34	theme	dextran	434:440	arg1	sodium					450:455	dextran sulfate sodium	434:455	dextran sulfate sodium (DSS)	434:461	To examine the anti-inflammatory, antioxidant and immunomodulatory activities of JSP in vivo, C57BL/6 mice were induced to develop ulcerative colitis by dextran sulfate sodium (DSS) and the roles of dietary JSP supplementation in modulating colitis were explored.
34528649	2	34	theme	dextran	434:440	arg1	DSS					458:460	DSS	458:460	DSS	458:460	To examine the anti-inflammatory, antioxidant and immunomodulatory activities of JSP in vivo, C57BL/6 mice were induced to develop ulcerative colitis by dextran sulfate sodium (DSS) and the roles of dietary JSP supplementation in modulating colitis were explored.
34528649	5	35	theme	signaling	937:945	arg1	pathways					947:954	NF-κB signaling pathways	931:954	NF-κB signaling pathways	931:954	JSP suppressed NF-κB signaling pathways as evidenced by lower levels of phosphorylated p65 and IKB.
34528649	1	36	attach	isolated	175:182	arg1	Kishinouye					210:219	Kishinouye	210:219	Kishinouye	210:219	Jellyfish skin polysaccharides (JSP) were isolated from Rhopilema esculentum Kishinouye and contained 55.11% polysaccharides and 2.26% uronic acid.
34528649	1	36	attach	isolated	175:182	arg2	JSP					165:167	JSP	165:167	JSP	165:167	Jellyfish skin polysaccharides (JSP) were isolated from Rhopilema esculentum Kishinouye and contained 55.11% polysaccharides and 2.26% uronic acid.
34528649	1	36	attach	isolated	175:182	arg2	polysaccharides					148:162	Jellyfish skin polysaccharides	133:162	Jellyfish skin polysaccharides (JSP)	133:168	Jellyfish skin polysaccharides (JSP) were isolated from Rhopilema esculentum Kishinouye and contained 55.11% polysaccharides and 2.26% uronic acid.
34528649	2	37	theme	anti-inflammatory	296:312	arg1	activities					348:357	the anti-inflammatory, antioxidant and immunomodulatory activities	292:357	the anti-inflammatory, antioxidant and immunomodulatory activities of JSP	292:364	To examine the anti-inflammatory, antioxidant and immunomodulatory activities of JSP in vivo, C57BL/6 mice were induced to develop ulcerative colitis by dextran sulfate sodium (DSS) and the roles of dietary JSP supplementation in modulating colitis were explored.
34528649	6	38	theme	gut	1146:1148	arg1	microbiota					1150:1159	the gut microbiota	1142:1159	the gut microbiota	1142:1159	Moreover, JSP supplementation enhanced the expression of tight junction proteins and mucins, and modulated the composition of the gut microbiota and the production of short-chain fatty acids.
34528649	0	39	theme	barrier	50:56	arg1	function					58:65	intestinal barrier function	39:65	intestinal barrier function	39:65	Jellyfish skin polysaccharides enhance intestinal barrier function and modulate the gut microbiota in mice with DSS-induced colitis.
34528649	4	40	theme	NO	824:825	arg1	level					827:831	NO level	824:831	NO level	824:831	JSP modulated oxidative stress and inflammatory responses, which was demonstrated by reduced MPO activity, NO level, and levels of pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 in mice.
34528649	2	41	theme	ulcerative	412:421	arg1	colitis					423:429	ulcerative colitis	412:429	ulcerative colitis by dextran sulfate sodium (DSS)	412:461	To examine the anti-inflammatory, antioxidant and immunomodulatory activities of JSP in vivo, C57BL/6 mice were induced to develop ulcerative colitis by dextran sulfate sodium (DSS) and the roles of dietary JSP supplementation in modulating colitis were explored.
34528649	0	42	theme	intestinal	39:48	arg1	function					58:65	intestinal barrier function	39:65	intestinal barrier function	39:65	Jellyfish skin polysaccharides enhance intestinal barrier function and modulate the gut microbiota in mice with DSS-induced colitis.
34528649	6	43	theme	JSP	1026:1028	arg1	supplementation					1030:1044	JSP supplementation	1026:1044	JSP supplementation	1026:1044	Moreover, JSP supplementation enhanced the expression of tight junction proteins and mucins, and modulated the composition of the gut microbiota and the production of short-chain fatty acids.
34528649	1	44	dep	%	240:240	arg1	polysaccharides					242:256	polysaccharides	242:256	55.11% polysaccharides	235:256	Jellyfish skin polysaccharides (JSP) were isolated from Rhopilema esculentum Kishinouye and contained 55.11% polysaccharides and 2.26% uronic acid.
34528649	2	45	theme	JSP	362:364	arg1	activities					348:357	the anti-inflammatory, antioxidant and immunomodulatory activities	292:357	the anti-inflammatory, antioxidant and immunomodulatory activities of JSP	292:364	To examine the anti-inflammatory, antioxidant and immunomodulatory activities of JSP in vivo, C57BL/6 mice were induced to develop ulcerative colitis by dextran sulfate sodium (DSS) and the roles of dietary JSP supplementation in modulating colitis were explored.
34528649	3	46	theme	colitis	589:595	arg1	symptoms					577:584	the symptoms	573:584	the symptoms of colitis in mice	573:603	JSP supplementation reduced the symptoms of colitis in mice, increased colon length, protected goblet cells, and improved intestinal epithelial integrity and permeability.
34528649	7	47	theme	JSP	1326:1328	arg1	potential					1313:1321	the potential	1309:1321	the potential of JSP as a nutritional supplement or adjunct strategy in preventing or ameliorating colitis	1309:1414	Taken together, these results reveal the anti-inflammatory effect of dietary JSP in vivo, suggesting the potential of JSP as a nutritional supplement or adjunct strategy in preventing or ameliorating colitis.
34528649	7	48	theme	JSP	1285:1287	arg1	effect					1267:1272	the anti-inflammatory effect	1245:1272	the anti-inflammatory effect of dietary JSP	1245:1287	Taken together, these results reveal the anti-inflammatory effect of dietary JSP in vivo, suggesting the potential of JSP as a nutritional supplement or adjunct strategy in preventing or ameliorating colitis.
34528649	6	49	theme	short-chain	1183:1193	arg1	acids					1201:1205	short-chain fatty acids	1183:1205	short-chain fatty acids	1183:1205	Moreover, JSP supplementation enhanced the expression of tight junction proteins and mucins, and modulated the composition of the gut microbiota and the production of short-chain fatty acids.
34528649	3	50	theme	intestinal	667:676	arg1	integrity					689:697	intestinal epithelial integrity	667:697	intestinal epithelial integrity	667:697	JSP supplementation reduced the symptoms of colitis in mice, increased colon length, protected goblet cells, and improved intestinal epithelial integrity and permeability.
34528649	3	51	theme	colon	616:620	arg1	length					622:627	colon length	616:627	colon length	616:627	JSP supplementation reduced the symptoms of colitis in mice, increased colon length, protected goblet cells, and improved intestinal epithelial integrity and permeability.
34528649	1	52	theme	Jellyfish	133:141	arg1	JSP					165:167	JSP	165:167	JSP	165:167	Jellyfish skin polysaccharides (JSP) were isolated from Rhopilema esculentum Kishinouye and contained 55.11% polysaccharides and 2.26% uronic acid.
34528649	1	52	theme	Jellyfish	133:141	arg1	polysaccharides					148:162	Jellyfish skin polysaccharides	133:162	Jellyfish skin polysaccharides (JSP)	133:168	Jellyfish skin polysaccharides (JSP) were isolated from Rhopilema esculentum Kishinouye and contained 55.11% polysaccharides and 2.26% uronic acid.
34528649	5	53	theme	phosphorylated	988:1001	arg1	p65					1003:1005	p65	1003:1005	p65	1003:1005	JSP suppressed NF-κB signaling pathways as evidenced by lower levels of phosphorylated p65 and IKB.
34528649	1	54	theme	skin	143:146	arg1	JSP					165:167	JSP	165:167	JSP	165:167	Jellyfish skin polysaccharides (JSP) were isolated from Rhopilema esculentum Kishinouye and contained 55.11% polysaccharides and 2.26% uronic acid.
34528649	1	54	theme	skin	143:146	arg1	polysaccharides					148:162	Jellyfish skin polysaccharides	133:162	Jellyfish skin polysaccharides (JSP)	133:168	Jellyfish skin polysaccharides (JSP) were isolated from Rhopilema esculentum Kishinouye and contained 55.11% polysaccharides and 2.26% uronic acid.
34528649	4	55	theme	oxidative	731:739	arg1	stress					741:746	oxidative stress	731:746	oxidative stress	731:746	JSP modulated oxidative stress and inflammatory responses, which was demonstrated by reduced MPO activity, NO level, and levels of pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 in mice.
34528649	2	56	theme	JSP	488:490	arg1	supplementation					492:506	dietary JSP supplementation	480:506	dietary JSP supplementation	480:506	To examine the anti-inflammatory, antioxidant and immunomodulatory activities of JSP in vivo, C57BL/6 mice were induced to develop ulcerative colitis by dextran sulfate sodium (DSS) and the roles of dietary JSP supplementation in modulating colitis were explored.
34528649	1	57	dep	%	266:266	arg1	acid					275:278	uronic acid	268:278	2.26% uronic acid	262:278	Jellyfish skin polysaccharides (JSP) were isolated from Rhopilema esculentum Kishinouye and contained 55.11% polysaccharides and 2.26% uronic acid.
34528649	5	58	theme	p65	1003:1005	arg1	levels					978:983	lower levels	972:983	lower levels of phosphorylated p65 and IKB	972:1013	JSP suppressed NF-κB signaling pathways as evidenced by lower levels of phosphorylated p65 and IKB.
34528649	2	59	theme	C57BL/6	375:381	arg1	mice					383:386	C57BL/6 mice	375:386	C57BL/6 mice	375:386	To examine the anti-inflammatory, antioxidant and immunomodulatory activities of JSP in vivo, C57BL/6 mice were induced to develop ulcerative colitis by dextran sulfate sodium (DSS) and the roles of dietary JSP supplementation in modulating colitis were explored.
34528649	3	60	from	symptoms	577:584	arg1	mice					600:603	mice	600:603	mice	600:603	JSP supplementation reduced the symptoms of colitis in mice, increased colon length, protected goblet cells, and improved intestinal epithelial integrity and permeability.
34528649	2	61	theme	dietary	480:486	arg1	supplementation					492:506	dietary JSP supplementation	480:506	dietary JSP supplementation	480:506	To examine the anti-inflammatory, antioxidant and immunomodulatory activities of JSP in vivo, C57BL/6 mice were induced to develop ulcerative colitis by dextran sulfate sodium (DSS) and the roles of dietary JSP supplementation in modulating colitis were explored.
34528649	7	62	theme	nutritional	1335:1345	arg1	strategy					1369:1376	a nutritional supplement or adjunct strategy	1333:1376	a nutritional supplement or adjunct strategy in preventing or ameliorating colitis	1333:1414	Taken together, these results reveal the anti-inflammatory effect of dietary JSP in vivo, suggesting the potential of JSP as a nutritional supplement or adjunct strategy in preventing or ameliorating colitis.
34528649	4	63	theme	MPO	810:812	arg1	activity					814:821	reduced MPO activity	802:821	reduced MPO activity	802:821	JSP modulated oxidative stress and inflammatory responses, which was demonstrated by reduced MPO activity, NO level, and levels of pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 in mice.
34528649	6	64	theme	mucins	1101:1106	arg1	expression					1059:1068	the expression	1055:1068	the expression of tight junction proteins and mucins	1055:1106	Moreover, JSP supplementation enhanced the expression of tight junction proteins and mucins, and modulated the composition of the gut microbiota and the production of short-chain fatty acids.
32978793	8	0	theme	absorption	1311:1320	arg1	capacity					1322:1329	water absorption capacity	1305:1329	water absorption capacity	1305:1329	According to the results, addition of gums increased moisture, ash, total color difference, water absorption capacity, solubility, failure force, rupture energy, elasticity module, and toughness when reduced lateral expansion was compared to the control.
32978793	8	1	theme	failure	1344:1350	arg1	force					1352:1356	failure force	1344:1356	failure force	1344:1356	According to the results, addition of gums increased moisture, ash, total color difference, water absorption capacity, solubility, failure force, rupture energy, elasticity module, and toughness when reduced lateral expansion was compared to the control.
32978793	0	2	theme	physicochemical	65:79	arg1	properties					107:116	physicochemical, mechanical, and textural properties	65:116	physicochemical, mechanical, and textural properties of extruded rice	65:133	Effect of carboxymethylcellulose and locust bean gums on some of physicochemical, mechanical, and textural properties of extruded rice.
32978793	3	3	with	combination	424:434	arg1	types					463:467	types	463:467	types of additives	463:480	In combination with different amounts and types of additives, this technique can result in functional food products for a broad range of people suffering from micronutrient deficiency.
32978793	3	3	with	combination	424:434	arg1	amounts					451:457	different amounts	441:457	different amounts	441:457	In combination with different amounts and types of additives, this technique can result in functional food products for a broad range of people suffering from micronutrient deficiency.
32978793	3	3	with	combination	424:434	arg1	additives					472:480	additives	472:480	additives	472:480	In combination with different amounts and types of additives, this technique can result in functional food products for a broad range of people suffering from micronutrient deficiency.
32978793	5	4	from	levels	829:834	arg1	effects					761:767	the effects	757:767	the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%)	757:854	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
32978793	4	5	theme	hydrocolloid	610:621	arg1	compounds					623:631	The hydrocolloid compounds	606:631	The hydrocolloid compounds	606:631	The hydrocolloid compounds are now employed to improve the texture and performance properties of many food products.
32978793	5	6	from	comparison	975:984	arg1	properties					944:953	chemical, physical, mechanical, and textural properties	899:953	chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice	899:1036	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
32978793	10	7	theme	tests	1694:1698	arg1	results					1661:1667	the results	1657:1667	the results of pre- and post-cooking tests	1657:1698	According to the results of pre- and post-cooking tests, the specimens containing locust (0.75%) and carboxymethylcellulose (0.75%) gums were the best treatments.
32978793	8	8	theme	gums	1251:1254	arg1	addition					1239:1246	addition	1239:1246	addition of gums	1239:1254	According to the results, addition of gums increased moisture, ash, total color difference, water absorption capacity, solubility, failure force, rupture energy, elasticity module, and toughness when reduced lateral expansion was compared to the control.
32978793	5	9	theme	different	819:827	arg1	levels					829:834	different levels	819:834	different levels (0.5, 0.75, and 1%)	819:854	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
32978793	5	9	theme	different	819:827	arg1	%					853:853	0.5, 0.75, and 1%	837:853	0.5, 0.75, and 1%	837:853	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
32978793	0	10	theme	mechanical	82:91	arg1	properties					107:116	physicochemical, mechanical, and textural properties	65:116	physicochemical, mechanical, and textural properties of extruded rice	65:133	Effect of carboxymethylcellulose and locust bean gums on some of physicochemical, mechanical, and textural properties of extruded rice.
32978793	2	11	with	product	353:359	arg1	taste					414:418	taste	414:418	taste	414:418	In this process, the raw material is forced through a tiny opening to be transformed into a product with a desired and unique shape, texture, color, and taste.
32978793	2	11	with	product	353:359	arg1	texture					394:400	texture	394:400	texture	394:400	In this process, the raw material is forced through a tiny opening to be transformed into a product with a desired and unique shape, texture, color, and taste.
32978793	2	11	with	product	353:359	arg1	shape					387:391	a desired and unique shape	366:391	a desired and unique shape	366:391	In this process, the raw material is forced through a tiny opening to be transformed into a product with a desired and unique shape, texture, color, and taste.
32978793	2	11	with	product	353:359	arg1	color					403:407	color	403:407	color	403:407	In this process, the raw material is forced through a tiny opening to be transformed into a product with a desired and unique shape, texture, color, and taste.
32978793	9	12	theme	cooking	1509:1515	arg1	process					1517:1523	the cooking process	1505:1523	the cooking process	1505:1523	Moreover, the addition of gums after the cooking process increased cooking loss, texture hardness, chewability, and elasticity of the specimens in comparison with the control.
32978793	4	13	theme	performance	677:687	arg1	properties					689:698	the texture and performance properties	661:698	the texture and performance properties of many food products	661:720	The hydrocolloid compounds are now employed to improve the texture and performance properties of many food products.
32978793	9	14	with	comparison	1615:1624	arg1	control					1635:1641	the control	1631:1641	the control	1631:1641	Moreover, the addition of gums after the cooking process increased cooking loss, texture hardness, chewability, and elasticity of the specimens in comparison with the control.
32978793	0	15	theme	textural	98:105	arg1	properties					107:116	physicochemical, mechanical, and textural properties	65:116	physicochemical, mechanical, and textural properties of extruded rice	65:133	Effect of carboxymethylcellulose and locust bean gums on some of physicochemical, mechanical, and textural properties of extruded rice.
32978793	5	16	with	combination	868:878	arg1	other					890:894	other	890:894	other	890:894	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
32978793	7	17	theme	multiple	1180:1187	arg1	%					1209:1209	α = 5%	1204:1209	α = 5%	1204:1209	Then the means were compared through Duncan's multiple range test at (α = 5%).
32978793	7	17	theme	multiple	1180:1187	arg1	at					1200:1201	Duncan's multiple range test at	1171:1201	Duncan's multiple range test at (α = 5%)	1171:1210	Then the means were compared through Duncan's multiple range test at (α = 5%).
32978793	4	18	theme	many	703:706	arg1	products					713:720	many food products	703:720	many food products	703:720	The hydrocolloid compounds are now employed to improve the texture and performance properties of many food products.
32978793	7	19	theme	range	1189:1193	arg1	%					1209:1209	α = 5%	1204:1209	α = 5%	1204:1209	Then the means were compared through Duncan's multiple range test at (α = 5%).
32978793	7	19	theme	range	1189:1193	arg1	at					1200:1201	Duncan's multiple range test at	1171:1201	Duncan's multiple range test at (α = 5%)	1171:1210	Then the means were compared through Duncan's multiple range test at (α = 5%).
32978793	1	20	theme	composite	151:159	arg1	Extrusion					136:144	Extrusion	136:144	Extrusion	136:144	Extrusion is a composite process consisting of many different unit operations such as mixing, cooking, pasting, and molding.
32978793	1	20	theme	composite	151:159	arg1	process					161:167	a composite process	149:167	a composite process consisting of many different unit operations such as mixing, cooking, pasting, and molding	149:258	Extrusion is a composite process consisting of many different unit operations such as mixing, cooking, pasting, and molding.
32978793	10	21	theme	best	1790:1793	arg1	specimens					1705:1713	the specimens	1701:1713	the specimens containing locust (0.75%) and carboxymethylcellulose (0.75%) gums	1701:1779	According to the results of pre- and post-cooking tests, the specimens containing locust (0.75%) and carboxymethylcellulose (0.75%) gums were the best treatments.
32978793	10	21	theme	best	1790:1793	arg1	treatments					1795:1804	the best treatments	1786:1804	the best treatments	1786:1804	According to the results of pre- and post-cooking tests, the specimens containing locust (0.75%) and carboxymethylcellulose (0.75%) gums were the best treatments.
32978793	9	22	theme	specimens	1602:1610	arg1	elasticity					1584:1593	elasticity	1584:1593	elasticity	1584:1593	Moreover, the addition of gums after the cooking process increased cooking loss, texture hardness, chewability, and elasticity of the specimens in comparison with the control.
32978793	9	22	theme	specimens	1602:1610	arg1	chewability					1567:1577	chewability	1567:1577	chewability	1567:1577	Moreover, the addition of gums after the cooking process increased cooking loss, texture hardness, chewability, and elasticity of the specimens in comparison with the control.
32978793	9	22	theme	specimens	1602:1610	arg1	loss					1543:1546	cooking loss	1535:1546	cooking loss	1535:1546	Moreover, the addition of gums after the cooking process increased cooking loss, texture hardness, chewability, and elasticity of the specimens in comparison with the control.
32978793	9	22	theme	specimens	1602:1610	arg1	hardness					1557:1564	texture hardness	1549:1564	texture hardness	1549:1564	Moreover, the addition of gums after the cooking process increased cooking loss, texture hardness, chewability, and elasticity of the specimens in comparison with the control.
32978793	10	23	theme	post-cooking	1681:1692	arg1	tests					1694:1698	pre- and post-cooking tests	1672:1698	pre- and post-cooking tests	1672:1698	According to the results of pre- and post-cooking tests, the specimens containing locust (0.75%) and carboxymethylcellulose (0.75%) gums were the best treatments.
32978793	5	24	theme	locust	799:804	arg1	gums					811:814	locust bean gums	799:814	locust bean gums	799:814	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
32978793	10	25	theme	pre-	1672:1675	arg1	tests					1694:1698	pre- and post-cooking tests	1672:1698	pre- and post-cooking tests	1672:1698	According to the results of pre- and post-cooking tests, the specimens containing locust (0.75%) and carboxymethylcellulose (0.75%) gums were the best treatments.
32978793	3	26	theme	different	441:449	arg1	amounts					451:457	different amounts	441:457	different amounts	441:457	In combination with different amounts and types of additives, this technique can result in functional food products for a broad range of people suffering from micronutrient deficiency.
32978793	3	26	theme	different	441:449	arg1	additives					472:480	additives	472:480	additives	472:480	In combination with different amounts and types of additives, this technique can result in functional food products for a broad range of people suffering from micronutrient deficiency.
32978793	9	27	theme	texture	1549:1555	arg1	hardness					1557:1564	texture hardness	1549:1564	texture hardness	1549:1564	Moreover, the addition of gums after the cooking process increased cooking loss, texture hardness, chewability, and elasticity of the specimens in comparison with the control.
32978793	8	28	theme	lateral	1421:1427	arg1	expansion					1429:1437	reduced lateral expansion	1413:1437	reduced lateral expansion	1413:1437	According to the results, addition of gums increased moisture, ash, total color difference, water absorption capacity, solubility, failure force, rupture energy, elasticity module, and toughness when reduced lateral expansion was compared to the control.
32978793	5	29	theme	bean	806:809	arg1	gums					811:814	locust bean gums	799:814	locust bean gums	799:814	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
32978793	2	30	theme	unique	380:385	arg1	shape					387:391	a desired and unique shape	366:391	a desired and unique shape	366:391	In this process, the raw material is forced through a tiny opening to be transformed into a product with a desired and unique shape, texture, color, and taste.
32978793	10	31	theme	carboxymethylcellulose	1745:1766	arg1	gums					1776:1779	locust (0.75%) and carboxymethylcellulose (0.75%) gums	1726:1779	gums	1776:1779	According to the results of pre- and post-cooking tests, the specimens containing locust (0.75%) and carboxymethylcellulose (0.75%) gums were the best treatments.
32978793	10	31	theme	carboxymethylcellulose	1745:1766	arg1	%					1773:1773	0.75%	1769:1773	0.75%	1769:1773	According to the results of pre- and post-cooking tests, the specimens containing locust (0.75%) and carboxymethylcellulose (0.75%) gums were the best treatments.
32978793	6	32	theme	test	1120:1123	arg1	results					1125:1131	the test results	1116:1131	the test results	1116:1131	A completely random design was used along with three replications to analyze the test results.
32978793	5	33	theme	mechanical	919:928	arg1	properties					944:953	chemical, physical, mechanical, and textural properties	899:953	chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice	899:1036	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
32978793	10	34	contain	containing	1715:1724	arg2	%					1738:1738	0.75%	1734:1738	0.75%	1734:1738	According to the results of pre- and post-cooking tests, the specimens containing locust (0.75%) and carboxymethylcellulose (0.75%) gums were the best treatments.
32978793	10	34	contain	containing	1715:1724	arg2	locust					1726:1731	locust (0.75%) and carboxymethylcellulose (0.75%) gums	1726:1779	locust	1726:1731	According to the results of pre- and post-cooking tests, the specimens containing locust (0.75%) and carboxymethylcellulose (0.75%) gums were the best treatments.
32978793	10	34	contain	containing	1715:1724	arg1	treatments					1795:1804	the best treatments	1786:1804	the best treatments	1786:1804	According to the results of pre- and post-cooking tests, the specimens containing locust (0.75%) and carboxymethylcellulose (0.75%) gums were the best treatments.
32978793	10	34	contain	containing	1715:1724	arg2	gums					1776:1779	locust (0.75%) and carboxymethylcellulose (0.75%) gums	1726:1779	gums	1776:1779	According to the results of pre- and post-cooking tests, the specimens containing locust (0.75%) and carboxymethylcellulose (0.75%) gums were the best treatments.
32978793	10	34	contain	containing	1715:1724	arg1	specimens					1705:1713	the specimens	1701:1713	the specimens containing locust (0.75%) and carboxymethylcellulose (0.75%) gums	1701:1779	According to the results of pre- and post-cooking tests, the specimens containing locust (0.75%) and carboxymethylcellulose (0.75%) gums were the best treatments.
32978793	10	34	contain	containing	1715:1724	arg2	%					1773:1773	0.75%	1769:1773	0.75%	1769:1773	According to the results of pre- and post-cooking tests, the specimens containing locust (0.75%) and carboxymethylcellulose (0.75%) gums were the best treatments.
32978793	5	35	theme	gums	811:814	arg1	effects					761:767	the effects	757:767	the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%)	757:854	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
32978793	7	36	theme	α	1204:1204	arg1	%					1209:1209	α = 5%	1204:1209	α = 5%	1204:1209	Then the means were compared through Duncan's multiple range test at (α = 5%).
32978793	7	36	theme	α	1204:1204	arg1	at					1200:1201	Duncan's multiple range test at	1171:1201	Duncan's multiple range test at (α = 5%)	1171:1210	Then the means were compared through Duncan's multiple range test at (α = 5%).
32978793	9	37	theme	gums	1494:1497	arg1	addition					1482:1489	the addition	1478:1489	the addition of gums after the cooking process	1478:1523	Moreover, the addition of gums after the cooking process increased cooking loss, texture hardness, chewability, and elasticity of the specimens in comparison with the control.
32978793	3	38	theme	additives	472:480	arg1	types					463:467	types	463:467	types of additives	463:480	In combination with different amounts and types of additives, this technique can result in functional food products for a broad range of people suffering from micronutrient deficiency.
32978793	3	38	theme	additives	472:480	arg1	amounts					451:457	different amounts	441:457	different amounts	441:457	In combination with different amounts and types of additives, this technique can result in functional food products for a broad range of people suffering from micronutrient deficiency.
32978793	3	38	theme	additives	472:480	arg1	additives					472:480	additives	472:480	additives	472:480	In combination with different amounts and types of additives, this technique can result in functional food products for a broad range of people suffering from micronutrient deficiency.
32978793	1	39	theme	many	183:186	arg1	mixing					222:227	mixing	222:227	mixing	222:227	Extrusion is a composite process consisting of many different unit operations such as mixing, cooking, pasting, and molding.
32978793	1	39	theme	many	183:186	arg1	pasting					239:245	pasting	239:245	pasting	239:245	Extrusion is a composite process consisting of many different unit operations such as mixing, cooking, pasting, and molding.
32978793	1	39	theme	many	183:186	arg1	molding					252:258	molding	252:258	molding	252:258	Extrusion is a composite process consisting of many different unit operations such as mixing, cooking, pasting, and molding.
32978793	1	39	theme	many	183:186	arg1	cooking					230:236	cooking	230:236	cooking	230:236	Extrusion is a composite process consisting of many different unit operations such as mixing, cooking, pasting, and molding.
32978793	1	39	theme	many	183:186	arg1	operations					203:212	many different unit operations	183:212	many different unit operations such as mixing, cooking, pasting, and molding	183:258	Extrusion is a composite process consisting of many different unit operations such as mixing, cooking, pasting, and molding.
32978793	2	40	theme	desired	368:374	arg1	shape					387:391	a desired and unique shape	366:391	a desired and unique shape	366:391	In this process, the raw material is forced through a tiny opening to be transformed into a product with a desired and unique shape, texture, color, and taste.
32978793	5	41	from	properties	944:953	arg1	comparison					975:984	comparison	975:984	comparison with the qualitative properties of the control rice	975:1036	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
32978793	6	42	theme	random	1052:1057	arg1	design					1059:1064	A completely random design	1039:1064	A completely random design	1039:1064	A completely random design was used along with three replications to analyze the test results.
32978793	4	43	theme	texture	665:671	arg1	properties					689:698	the texture and performance properties	661:698	the texture and performance properties of many food products	661:720	The hydrocolloid compounds are now employed to improve the texture and performance properties of many food products.
32978793	8	44	theme	elasticity	1375:1384	arg1	module					1386:1391	elasticity module	1375:1391	elasticity module	1375:1391	According to the results, addition of gums increased moisture, ash, total color difference, water absorption capacity, solubility, failure force, rupture energy, elasticity module, and toughness when reduced lateral expansion was compared to the control.
32978793	1	45	theme	different	188:196	arg1	mixing					222:227	mixing	222:227	mixing	222:227	Extrusion is a composite process consisting of many different unit operations such as mixing, cooking, pasting, and molding.
32978793	1	45	theme	different	188:196	arg1	pasting					239:245	pasting	239:245	pasting	239:245	Extrusion is a composite process consisting of many different unit operations such as mixing, cooking, pasting, and molding.
32978793	1	45	theme	different	188:196	arg1	molding					252:258	molding	252:258	molding	252:258	Extrusion is a composite process consisting of many different unit operations such as mixing, cooking, pasting, and molding.
32978793	1	45	theme	different	188:196	arg1	cooking					230:236	cooking	230:236	cooking	230:236	Extrusion is a composite process consisting of many different unit operations such as mixing, cooking, pasting, and molding.
32978793	1	45	theme	different	188:196	arg1	operations					203:212	many different unit operations	183:212	many different unit operations such as mixing, cooking, pasting, and molding	183:258	Extrusion is a composite process consisting of many different unit operations such as mixing, cooking, pasting, and molding.
32978793	0	46	theme	bean	44:47	arg1	Effect					0:5	Effect	0:5	Effect of carboxymethylcellulose and locust bean	0:47	Effect of carboxymethylcellulose and locust bean gums on some of physicochemical, mechanical, and textural properties of extruded rice.
32978793	0	47	theme	rice	130:133	arg1	properties					107:116	physicochemical, mechanical, and textural properties	65:116	physicochemical, mechanical, and textural properties of extruded rice	65:133	Effect of carboxymethylcellulose and locust bean gums on some of physicochemical, mechanical, and textural properties of extruded rice.
32978793	3	48	theme	people	558:563	arg1	range					549:553	a broad range	541:553	a broad range of people suffering from micronutrient deficiency	541:603	In combination with different amounts and types of additives, this technique can result in functional food products for a broad range of people suffering from micronutrient deficiency.
32978793	5	49	theme	chemical	899:906	arg1	properties					944:953	chemical, physical, mechanical, and textural properties	899:953	chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice	899:1036	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
32978793	7	50	theme	test	1195:1198	arg1	%					1209:1209	α = 5%	1204:1209	α = 5%	1204:1209	Then the means were compared through Duncan's multiple range test at (α = 5%).
32978793	7	50	theme	test	1195:1198	arg1	at					1200:1201	Duncan's multiple range test at	1171:1201	Duncan's multiple range test at (α = 5%)	1171:1210	Then the means were compared through Duncan's multiple range test at (α = 5%).
32978793	1	51	theme	unit	198:201	arg1	mixing					222:227	mixing	222:227	mixing	222:227	Extrusion is a composite process consisting of many different unit operations such as mixing, cooking, pasting, and molding.
32978793	1	51	theme	unit	198:201	arg1	pasting					239:245	pasting	239:245	pasting	239:245	Extrusion is a composite process consisting of many different unit operations such as mixing, cooking, pasting, and molding.
32978793	1	51	theme	unit	198:201	arg1	molding					252:258	molding	252:258	molding	252:258	Extrusion is a composite process consisting of many different unit operations such as mixing, cooking, pasting, and molding.
32978793	1	51	theme	unit	198:201	arg1	cooking					230:236	cooking	230:236	cooking	230:236	Extrusion is a composite process consisting of many different unit operations such as mixing, cooking, pasting, and molding.
32978793	1	51	theme	unit	198:201	arg1	operations					203:212	many different unit operations	183:212	many different unit operations such as mixing, cooking, pasting, and molding	183:258	Extrusion is a composite process consisting of many different unit operations such as mixing, cooking, pasting, and molding.
32978793	0	52	theme	locust	37:42	arg1	bean					44:47	locust bean	37:47	locust bean	37:47	Effect of carboxymethylcellulose and locust bean gums on some of physicochemical, mechanical, and textural properties of extruded rice.
32978793	5	53	theme	control	1025:1031	arg1	rice					1033:1036	the control rice	1021:1036	the control rice	1021:1036	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
32978793	0	54	theme	extruded	121:128	arg1	rice					130:133	extruded rice	121:133	extruded rice	121:133	Effect of carboxymethylcellulose and locust bean gums on some of physicochemical, mechanical, and textural properties of extruded rice.
32978793	3	55	theme	broad	543:547	arg1	range					549:553	a broad range	541:553	a broad range of people suffering from micronutrient deficiency	541:603	In combination with different amounts and types of additives, this technique can result in functional food products for a broad range of people suffering from micronutrient deficiency.
32978793	3	56	theme	functional	512:521	arg1	products					528:535	functional food products	512:535	functional food products for a broad range of people suffering from micronutrient deficiency	512:603	In combination with different amounts and types of additives, this technique can result in functional food products for a broad range of people suffering from micronutrient deficiency.
32978793	5	57	theme	carboxymethylcellulose	772:793	arg1	effects					761:767	the effects	757:767	the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%)	757:854	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
32978793	5	58	theme	rice	1033:1036	arg1	properties					1007:1016	the qualitative properties	991:1016	the qualitative properties of the control rice	991:1036	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
32978793	5	59	theme	rice	967:970	arg1	properties					944:953	chemical, physical, mechanical, and textural properties	899:953	chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice	899:1036	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
32978793	5	60	theme	physical	909:916	arg1	properties					944:953	chemical, physical, mechanical, and textural properties	899:953	chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice	899:1036	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
32978793	9	61	theme	cooking	1535:1541	arg1	loss					1543:1546	cooking loss	1535:1546	cooking loss	1535:1546	Moreover, the addition of gums after the cooking process increased cooking loss, texture hardness, chewability, and elasticity of the specimens in comparison with the control.
32978793	3	62	theme	micronutrient	580:592	arg1	deficiency					594:603	micronutrient deficiency	580:603	micronutrient deficiency	580:603	In combination with different amounts and types of additives, this technique can result in functional food products for a broad range of people suffering from micronutrient deficiency.
32978793	5	63	theme	extruded	958:965	arg1	rice					967:970	extruded rice	958:970	extruded rice in comparison with the qualitative properties of the control rice	958:1036	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
32978793	6	64	used	used	1070:1073	arg2	design					1059:1064	A completely random design	1039:1064	A completely random design	1039:1064	A completely random design was used along with three replications to analyze the test results.
32978793	7	65	theme	=	1206:1206	arg1	%					1209:1209	α = 5%	1204:1209	α = 5%	1204:1209	Then the means were compared through Duncan's multiple range test at (α = 5%).
32978793	7	65	theme	=	1206:1206	arg1	at					1200:1201	Duncan's multiple range test at	1171:1201	Duncan's multiple range test at (α = 5%)	1171:1210	Then the means were compared through Duncan's multiple range test at (α = 5%).
32978793	8	66	theme	color	1287:1291	arg1	difference					1293:1302	total color difference	1281:1302	total color difference	1281:1302	According to the results, addition of gums increased moisture, ash, total color difference, water absorption capacity, solubility, failure force, rupture energy, elasticity module, and toughness when reduced lateral expansion was compared to the control.
32978793	4	67	theme	products	713:720	arg1	properties					689:698	the texture and performance properties	661:698	the texture and performance properties of many food products	661:720	The hydrocolloid compounds are now employed to improve the texture and performance properties of many food products.
32978793	8	68	theme	reduced	1413:1419	arg1	expansion					1429:1437	reduced lateral expansion	1413:1437	reduced lateral expansion	1413:1437	According to the results, addition of gums increased moisture, ash, total color difference, water absorption capacity, solubility, failure force, rupture energy, elasticity module, and toughness when reduced lateral expansion was compared to the control.
32978793	8	69	theme	water	1305:1309	arg1	capacity					1322:1329	water absorption capacity	1305:1329	water absorption capacity	1305:1329	According to the results, addition of gums increased moisture, ash, total color difference, water absorption capacity, solubility, failure force, rupture energy, elasticity module, and toughness when reduced lateral expansion was compared to the control.
32978793	4	70	theme	food	708:711	arg1	products					713:720	many food products	703:720	many food products	703:720	The hydrocolloid compounds are now employed to improve the texture and performance properties of many food products.
32978793	2	71	theme	raw	282:284	arg1	material					286:293	the raw material	278:293	the raw material	278:293	In this process, the raw material is forced through a tiny opening to be transformed into a product with a desired and unique shape, texture, color, and taste.
32978793	8	72	theme	total	1281:1285	arg1	difference					1293:1302	total color difference	1281:1302	total color difference	1281:1302	According to the results, addition of gums increased moisture, ash, total color difference, water absorption capacity, solubility, failure force, rupture energy, elasticity module, and toughness when reduced lateral expansion was compared to the control.
32978793	5	73	theme	textural	935:942	arg1	properties					944:953	chemical, physical, mechanical, and textural properties	899:953	chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice	899:1036	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
32978793	3	74	theme	food	523:526	arg1	products					528:535	functional food products	512:535	functional food products for a broad range of people suffering from micronutrient deficiency	512:603	In combination with different amounts and types of additives, this technique can result in functional food products for a broad range of people suffering from micronutrient deficiency.
32978793	8	75	theme	rupture	1359:1365	arg1	energy					1367:1372	rupture energy	1359:1372	rupture energy	1359:1372	According to the results, addition of gums increased moisture, ash, total color difference, water absorption capacity, solubility, failure force, rupture energy, elasticity module, and toughness when reduced lateral expansion was compared to the control.
32978793	5	76	with	comparison	975:984	arg1	properties					1007:1016	the qualitative properties	991:1016	the qualitative properties of the control rice	991:1036	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
32978793	5	77	theme	qualitative	995:1005	arg1	properties					1007:1016	the qualitative properties	991:1016	the qualitative properties of the control rice	991:1036	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
32978793	0	78	theme	carboxymethylcellulose	10:31	arg1	Effect					0:5	Effect	0:5	Effect of carboxymethylcellulose and locust bean	0:47	Effect of carboxymethylcellulose and locust bean gums on some of physicochemical, mechanical, and textural properties of extruded rice.
32978793	2	79	theme	tiny	315:318	arg1	opening					320:326	a tiny opening	313:326	a tiny opening to be transformed into a product with a desired and unique shape, texture, color, and taste	313:418	In this process, the raw material is forced through a tiny opening to be transformed into a product with a desired and unique shape, texture, color, and taste.
32978793	5	80	from	rice	967:970	arg1	comparison					975:984	comparison	975:984	comparison with the qualitative properties of the control rice	975:1036	Therefore, this study scrutinized the effects of carboxymethylcellulose and locust bean gums at different levels (0.5, 0.75, and 1%) alone or in combination with each other on chemical, physical, mechanical, and textural properties of extruded rice in comparison with the qualitative properties of the control rice.
33600374	0	0	theme	mussel	92:97	arg1	shell					99:103	waste mussel shell and waste active sludge char	86:132	shell	99:103	Adsorption of reactive blue 49 onto cross-linked chitosan-based composites containing waste mussel shell and waste active sludge char.
33600374	4	1	theme	temperature	838:848	arg1	effects					706:712	The effects	702:712	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites	702:934	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites were investigated.
33600374	4	2	from	effects	706:712	arg1	adsorption					888:897	Reactive Blue 49 (RB49) adsorption	864:897	Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites	864:934	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites were investigated.
33600374	1	3	theme	shell	170:174	arg1	C/WMS					177:181	Cross-linked chitosan/waste mussel shell (C/WMS)	135:182	Cross-linked chitosan/waste mussel shell (C/WMS)	135:182	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	5	4	dep	C/WMS	995:999	arg1	composites					1016:1025	composites	1016:1025	composites	1016:1025	The maximum RB49 adsorption capacity of C/WMS and C/WMS/WASC composites was 54.7 and 38.8 mg/g, respectively.
33600374	0	5	theme	waste	86:90	arg1	shell					99:103	waste mussel shell and waste active sludge char	86:132	shell	99:103	Adsorption of reactive blue 49 onto cross-linked chitosan-based composites containing waste mussel shell and waste active sludge char.
33600374	5	6	theme	RB49	967:970	arg1	mg/g					1045:1048	54.7 and 38.8 mg/g	1031:1048	54.7 and 38.8 mg/g	1031:1048	The maximum RB49 adsorption capacity of C/WMS and C/WMS/WASC composites was 54.7 and 38.8 mg/g, respectively.
33600374	5	6	theme	RB49	967:970	arg1	capacity					983:990	The maximum RB49 adsorption capacity	955:990	The maximum RB49 adsorption capacity of C/WMS and C/WMS/WASC composites	955:1025	The maximum RB49 adsorption capacity of C/WMS and C/WMS/WASC composites was 54.7 and 38.8 mg/g, respectively.
33600374	6	7	theme	isotherm	1116:1123	arg1	models					1125:1130	kinetic and isotherm models	1104:1130	kinetic and isotherm models	1104:1130	The experimental data were analyzed by kinetic and isotherm models.
33600374	4	8	theme	adsorbent	756:764	arg1	ml					788:789	0.01-0.1 g/50 ml	774:789	0.01-0.1 g/50 ml	774:789	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites were investigated.
33600374	4	8	theme	adsorbent	756:764	arg1	dosage					766:771	adsorbent dosage	756:771	adsorbent dosage (0.01-0.1 g/50 ml)	756:790	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites were investigated.
33600374	5	9	theme	adsorption	972:981	arg1	mg/g					1045:1048	54.7 and 38.8 mg/g	1031:1048	54.7 and 38.8 mg/g	1031:1048	The maximum RB49 adsorption capacity of C/WMS and C/WMS/WASC composites was 54.7 and 38.8 mg/g, respectively.
33600374	5	9	theme	adsorption	972:981	arg1	capacity					983:990	The maximum RB49 adsorption capacity	955:990	The maximum RB49 adsorption capacity of C/WMS and C/WMS/WASC composites	955:1025	The maximum RB49 adsorption capacity of C/WMS and C/WMS/WASC composites was 54.7 and 38.8 mg/g, respectively.
33600374	2	10	dep	C/WMS	438:442	arg1	composites					459:468	composites	459:468	composites	459:468	The quantities of chitosan, WMS, and WASC used for the C/WMS and C/WMS/WASC composites were 1:1 and 1:0.5:0.5, respectively.
33600374	2	10	dep	C/WMS	438:442	arg1	the					434:436	the	434:436	the	434:436	The quantities of chitosan, WMS, and WASC used for the C/WMS and C/WMS/WASC composites were 1:1 and 1:0.5:0.5, respectively.
33600374	8	11	theme	effective	1406:1414	arg1	adsorbents					1416:1425	effective adsorbents	1406:1425	effective adsorbents for the treatment of waste water contaminated with RB49	1406:1481	All results showed the preparative adsorbents can be used as effective adsorbents for the treatment of waste water contaminated with RB49 since they are low cost and eco-friendly for the environment.
33600374	4	12	theme	concentration	805:817	arg1	effects					706:712	The effects	702:712	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites	702:934	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites were investigated.
33600374	1	13	dep	sludge	317:322	arg1	WAS					325:327	WAS	325:327	WAS	325:327	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	4	14	theme	Reactive	864:871	arg1	adsorption					888:897	Reactive Blue 49 (RB49) adsorption	864:897	Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites	864:934	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites were investigated.
33600374	6	15	theme	kinetic	1104:1110	arg1	models					1125:1130	kinetic and isotherm models	1104:1130	kinetic and isotherm models	1104:1130	The experimental data were analyzed by kinetic and isotherm models.
33600374	7	16	theme	adsorption	1261:1270	arg1	kinetics					1272:1279	the adsorption kinetics	1257:1279	the adsorption kinetics for both adsorbents	1257:1299	The Freundlich isotherm was a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites, and the adsorption kinetics for both adsorbents were the pseudo-second-order rate equation.
33600374	7	16	theme	adsorption	1261:1270	arg1	equation					1335:1342	the pseudo-second-order rate equation	1306:1342	the pseudo-second-order rate equation	1306:1342	The Freundlich isotherm was a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites, and the adsorption kinetics for both adsorbents were the pseudo-second-order rate equation.
33600374	0	17	theme	active	115:120	arg1	char					129:132	waste mussel shell and waste active sludge char	86:132	char	129:132	Adsorption of reactive blue 49 onto cross-linked chitosan-based composites containing waste mussel shell and waste active sludge char.
33600374	3	18	theme	Emmett	668:673	arg1	analyzer					692:699	a Brunauer, Emmett and Teller (BET) analyzer	656:699	a Brunauer, Emmett and Teller (BET) analyzer	656:699	The two adsorbents were characterized for their quality by a scanning electron microscope (SEM), an energy-dispersive X-ray spectroscopy (EDX), and a Brunauer, Emmett and Teller (BET) analyzer.
33600374	4	19	theme	pH	746:747	arg1	effects					706:712	The effects	702:712	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites	702:934	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites were investigated.
33600374	2	20	theme	chitosan	401:408	arg1	1:1					475:477	1:1	475:477	1:1	475:477	The quantities of chitosan, WMS, and WASC used for the C/WMS and C/WMS/WASC composites were 1:1 and 1:0.5:0.5, respectively.
33600374	2	20	theme	chitosan	401:408	arg1	quantities					387:396	The quantities	383:396	The quantities of chitosan, WMS, and WASC used for the C/WMS and C/WMS/WASC composites	383:468	The quantities of chitosan, WMS, and WASC used for the C/WMS and C/WMS/WASC composites were 1:1 and 1:0.5:0.5, respectively.
33600374	1	21	theme	C/WMS	177:181	arg1	composites					254:263	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites	135:263	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites	135:263	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	0	22	theme	waste	109:113	arg1	char					129:132	waste mussel shell and waste active sludge char	86:132	char	129:132	Adsorption of reactive blue 49 onto cross-linked chitosan-based composites containing waste mussel shell and waste active sludge char.
33600374	4	23	theme	time	725:728	arg1	effects					706:712	The effects	702:712	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites	702:934	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites were investigated.
33600374	2	24	theme	WASC	420:423	arg1	1:1					475:477	1:1	475:477	1:1	475:477	The quantities of chitosan, WMS, and WASC used for the C/WMS and C/WMS/WASC composites were 1:1 and 1:0.5:0.5, respectively.
33600374	2	24	theme	WASC	420:423	arg1	quantities					387:396	The quantities	383:396	The quantities of chitosan, WMS, and WASC used for the C/WMS and C/WMS/WASC composites	383:468	The quantities of chitosan, WMS, and WASC used for the C/WMS and C/WMS/WASC composites were 1:1 and 1:0.5:0.5, respectively.
33600374	8	25	theme	preparative	1368:1378	arg1	adsorbents					1380:1389	the preparative adsorbents	1364:1389	the preparative adsorbents can be used as effective adsorbents for the treatment of waste water contaminated with RB49 since they are low cost and eco-friendly for the environment	1364:1542	All results showed the preparative adsorbents can be used as effective adsorbents for the treatment of waste water contaminated with RB49 since they are low cost and eco-friendly for the environment.
33600374	0	26	link	cross-linked	36:47	arg1	composites					64:73	cross-linked chitosan-based composites	36:73	cross-linked chitosan-based composites	36:73	Adsorption of reactive blue 49 onto cross-linked chitosan-based composites containing waste mussel shell and waste active sludge char.
33600374	7	27	from	data	1193:1196	arg1	C/WMS/WASC					1230:1239	C/WMS/WASC	1230:1239	C/WMS/WASC	1230:1239	The Freundlich isotherm was a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites, and the adsorption kinetics for both adsorbents were the pseudo-second-order rate equation.
33600374	7	27	from	data	1193:1196	arg1	C/WMS					1220:1224	C/WMS	1220:1224	C/WMS	1220:1224	The Freundlich isotherm was a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites, and the adsorption kinetics for both adsorbents were the pseudo-second-order rate equation.
33600374	7	28	dep	C/WMS	1220:1224	arg1	composites					1241:1250	composites	1241:1250	composites	1241:1250	The Freundlich isotherm was a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites, and the adsorption kinetics for both adsorbents were the pseudo-second-order rate equation.
33600374	1	29	theme	waste	284:288	arg1	shell					297:301	waste mussel shell	284:301	waste mussel shell	284:301	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	4	30	dep	C/WMS	904:908	arg1	composites					925:934	composites	925:934	composites	925:934	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites were investigated.
33600374	1	31	theme	chitosan/waste	188:201	arg1	C/WMS/WASC					242:251	C/WMS/WASC	242:251	C/WMS/WASC	242:251	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	1	31	theme	chitosan/waste	188:201	arg1	char					236:239	chitosan/waste mussel shell/waste active sludge char	188:239	chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC)	188:252	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	1	32	theme	mussel	290:295	arg1	shell					297:301	waste mussel shell	284:301	waste mussel shell	284:301	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	6	33	theme	experimental	1069:1080	arg1	data					1082:1085	The experimental data	1065:1085	The experimental data	1065:1085	The experimental data were analyzed by kinetic and isotherm models.
33600374	1	34	theme	mussel	203:208	arg1	C/WMS/WASC					242:251	C/WMS/WASC	242:251	C/WMS/WASC	242:251	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	1	34	theme	mussel	203:208	arg1	char					236:239	chitosan/waste mussel shell/waste active sludge char	188:239	chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC)	188:252	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	2	35	theme	WMS	411:413	arg1	1:1					475:477	1:1	475:477	1:1	475:477	The quantities of chitosan, WMS, and WASC used for the C/WMS and C/WMS/WASC composites were 1:1 and 1:0.5:0.5, respectively.
33600374	2	35	theme	WMS	411:413	arg1	quantities					387:396	The quantities	383:396	The quantities of chitosan, WMS, and WASC used for the C/WMS and C/WMS/WASC composites	383:468	The quantities of chitosan, WMS, and WASC used for the C/WMS and C/WMS/WASC composites were 1:1 and 1:0.5:0.5, respectively.
33600374	5	36	theme	maximum	959:965	arg1	mg/g					1045:1048	54.7 and 38.8 mg/g	1031:1048	54.7 and 38.8 mg/g	1031:1048	The maximum RB49 adsorption capacity of C/WMS and C/WMS/WASC composites was 54.7 and 38.8 mg/g, respectively.
33600374	5	36	theme	maximum	959:965	arg1	capacity					983:990	The maximum RB49 adsorption capacity	955:990	The maximum RB49 adsorption capacity of C/WMS and C/WMS/WASC composites	955:1025	The maximum RB49 adsorption capacity of C/WMS and C/WMS/WASC composites was 54.7 and 38.8 mg/g, respectively.
33600374	0	37	theme	sludge	122:127	arg1	char					129:132	waste mussel shell and waste active sludge char	86:132	char	129:132	Adsorption of reactive blue 49 onto cross-linked chitosan-based composites containing waste mussel shell and waste active sludge char.
33600374	7	38	from	adsorption	1206:1215	arg1	C/WMS/WASC					1230:1239	C/WMS/WASC	1230:1239	C/WMS/WASC	1230:1239	The Freundlich isotherm was a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites, and the adsorption kinetics for both adsorbents were the pseudo-second-order rate equation.
33600374	7	38	from	adsorption	1206:1215	arg1	C/WMS					1220:1224	C/WMS	1220:1224	C/WMS	1220:1224	The Freundlich isotherm was a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites, and the adsorption kinetics for both adsorbents were the pseudo-second-order rate equation.
33600374	1	39	theme	shell/waste	210:220	arg1	C/WMS/WASC					242:251	C/WMS/WASC	242:251	C/WMS/WASC	242:251	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	1	39	theme	shell/waste	210:220	arg1	char					236:239	chitosan/waste mussel shell/waste active sludge char	188:239	chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC)	188:252	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	3	40	theme	Teller	679:684	arg1	analyzer					692:699	a Brunauer, Emmett and Teller (BET) analyzer	656:699	a Brunauer, Emmett and Teller (BET) analyzer	656:699	The two adsorbents were characterized for their quality by a scanning electron microscope (SEM), an energy-dispersive X-ray spectroscopy (EDX), and a Brunauer, Emmett and Teller (BET) analyzer.
33600374	4	41	theme	dye	801:803	arg1	concentration					805:817	initial dye concentration	793:817	initial dye concentration (20-100 mg/L)	793:831	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites were investigated.
33600374	4	41	theme	dye	801:803	arg1	mg/L					827:830	20-100 mg/L	820:830	20-100 mg/L	820:830	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites were investigated.
33600374	4	42	theme	contact	717:723	arg1	time					725:728	contact time	717:728	contact time (0-1,620 mins)	717:743	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites were investigated.
33600374	4	42	theme	contact	717:723	arg1	mins					739:742	0-1,620 mins	731:742	0-1,620 mins	731:742	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites were investigated.
33600374	7	43	theme	rate	1330:1333	arg1	equation					1335:1342	the pseudo-second-order rate equation	1306:1342	the pseudo-second-order rate equation	1306:1342	The Freundlich isotherm was a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites, and the adsorption kinetics for both adsorbents were the pseudo-second-order rate equation.
33600374	7	43	theme	rate	1330:1333	arg1	kinetics					1272:1279	the adsorption kinetics	1257:1279	the adsorption kinetics for both adsorbents	1257:1299	The Freundlich isotherm was a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites, and the adsorption kinetics for both adsorbents were the pseudo-second-order rate equation.
33600374	1	44	theme	active	222:227	arg1	C/WMS/WASC					242:251	C/WMS/WASC	242:251	C/WMS/WASC	242:251	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	1	44	theme	active	222:227	arg1	char					236:239	chitosan/waste mussel shell/waste active sludge char	188:239	chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC)	188:252	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	0	45	theme	49	28:29	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption of reactive blue 49 onto cross-linked chitosan-based composites	0:73	Adsorption of reactive blue 49 onto cross-linked chitosan-based composites containing waste mussel shell and waste active sludge char.
33600374	1	46	theme	waste	304:308	arg1	sludge					317:322	waste active sludge	304:322	waste active sludge (WAS)	304:328	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	4	47	theme	initial	793:799	arg1	concentration					805:817	initial dye concentration	793:817	initial dye concentration (20-100 mg/L)	793:831	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites were investigated.
33600374	4	47	theme	initial	793:799	arg1	mg/L					827:830	20-100 mg/L	820:830	20-100 mg/L	820:830	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites were investigated.
33600374	7	48	theme	good	1163:1166	arg1	isotherm					1148:1155	The Freundlich isotherm	1133:1155	The Freundlich isotherm	1133:1155	The Freundlich isotherm was a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites, and the adsorption kinetics for both adsorbents were the pseudo-second-order rate equation.
33600374	7	48	theme	good	1163:1166	arg1	fit					1168:1170	a good fit	1161:1170	a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites	1161:1250	The Freundlich isotherm was a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites, and the adsorption kinetics for both adsorbents were the pseudo-second-order rate equation.
33600374	1	49	theme	sludge	229:234	arg1	C/WMS/WASC					242:251	C/WMS/WASC	242:251	C/WMS/WASC	242:251	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	1	49	theme	sludge	229:234	arg1	char					236:239	chitosan/waste mussel shell/waste active sludge char	188:239	chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC)	188:252	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	0	50	theme	chitosan-based	49:62	arg1	composites					64:73	cross-linked chitosan-based composites	36:73	cross-linked chitosan-based composites	36:73	Adsorption of reactive blue 49 onto cross-linked chitosan-based composites containing waste mussel shell and waste active sludge char.
33600374	1	51	theme	active	310:315	arg1	sludge					317:322	waste active sludge	304:322	waste active sludge (WAS)	304:328	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	0	52	theme	cross-linked	36:47	arg1	composites					64:73	cross-linked chitosan-based composites	36:73	cross-linked chitosan-based composites	36:73	Adsorption of reactive blue 49 onto cross-linked chitosan-based composites containing waste mussel shell and waste active sludge char.
33600374	5	53	theme	C/WMS	995:999	arg1	mg/g					1045:1048	54.7 and 38.8 mg/g	1031:1048	54.7 and 38.8 mg/g	1031:1048	The maximum RB49 adsorption capacity of C/WMS and C/WMS/WASC composites was 54.7 and 38.8 mg/g, respectively.
33600374	5	53	theme	C/WMS	995:999	arg1	capacity					983:990	The maximum RB49 adsorption capacity	955:990	The maximum RB49 adsorption capacity of C/WMS and C/WMS/WASC composites	955:1025	The maximum RB49 adsorption capacity of C/WMS and C/WMS/WASC composites was 54.7 and 38.8 mg/g, respectively.
33600374	1	54	link	Cross-linked	135:146	arg1	C/WMS					177:181	Cross-linked chitosan/waste mussel shell (C/WMS)	135:182	Cross-linked chitosan/waste mussel shell (C/WMS)	135:182	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	7	55	theme	adsorption	1206:1215	arg1	data					1193:1196	the experimental data	1176:1196	the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites	1176:1250	The Freundlich isotherm was a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites, and the adsorption kinetics for both adsorbents were the pseudo-second-order rate equation.
33600374	7	56	theme	RB49	1201:1204	arg1	adsorption					1206:1215	RB49 adsorption	1201:1215	RB49 adsorption on C/WMS and C/WMS/WASC composites	1201:1250	The Freundlich isotherm was a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites, and the adsorption kinetics for both adsorbents were the pseudo-second-order rate equation.
33600374	4	57	theme	dosage	766:771	arg1	effects					706:712	The effects	702:712	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites	702:934	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites were investigated.
33600374	7	58	from	C/WMS	1220:1224	arg1	data					1193:1196	the experimental data	1176:1196	the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites	1176:1250	The Freundlich isotherm was a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites, and the adsorption kinetics for both adsorbents were the pseudo-second-order rate equation.
33600374	3	59	dep	Emmett	668:673	arg1	a					656:656	a	656:656	a	656:656	The two adsorbents were characterized for their quality by a scanning electron microscope (SEM), an energy-dispersive X-ray spectroscopy (EDX), and a Brunauer, Emmett and Teller (BET) analyzer.
33600374	4	60	theme	Blue	873:876	arg1	adsorption					888:897	Reactive Blue 49 (RB49) adsorption	864:897	Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites	864:934	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites were investigated.
33600374	7	61	theme	experimental	1180:1191	arg1	data					1193:1196	the experimental data	1176:1196	the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites	1176:1250	The Freundlich isotherm was a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites, and the adsorption kinetics for both adsorbents were the pseudo-second-order rate equation.
33600374	1	62	theme	char	236:239	arg1	composites					254:263	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites	135:263	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites	135:263	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	4	63	theme	g/50	783:786	arg1	ml					788:789	0.01-0.1 g/50 ml	774:789	0.01-0.1 g/50 ml	774:789	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites were investigated.
33600374	4	63	theme	g/50	783:786	arg1	dosage					766:771	adsorbent dosage	756:771	adsorbent dosage (0.01-0.1 g/50 ml)	756:790	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites were investigated.
33600374	3	64	theme	energy-dispersive	608:624	arg1	EDX					646:648	EDX	646:648	EDX	646:648	The two adsorbents were characterized for their quality by a scanning electron microscope (SEM), an energy-dispersive X-ray spectroscopy (EDX), and a Brunauer, Emmett and Teller (BET) analyzer.
33600374	3	64	theme	energy-dispersive	608:624	arg1	spectroscopy					632:643	an energy-dispersive X-ray spectroscopy	605:643	an energy-dispersive X-ray spectroscopy (EDX)	605:649	The two adsorbents were characterized for their quality by a scanning electron microscope (SEM), an energy-dispersive X-ray spectroscopy (EDX), and a Brunauer, Emmett and Teller (BET) analyzer.
33600374	1	65	theme	Cross-linked	135:146	arg1	C/WMS					177:181	Cross-linked chitosan/waste mussel shell (C/WMS)	135:182	Cross-linked chitosan/waste mussel shell (C/WMS)	135:182	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	7	66	from	C/WMS/WASC	1230:1239	arg1	data					1193:1196	the experimental data	1176:1196	the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites	1176:1250	The Freundlich isotherm was a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites, and the adsorption kinetics for both adsorbents were the pseudo-second-order rate equation.
33600374	8	67	theme	low	1498:1500	arg1	they					1489:1492	they	1489:1492	they	1489:1492	All results showed the preparative adsorbents can be used as effective adsorbents for the treatment of waste water contaminated with RB49 since they are low cost and eco-friendly for the environment.
33600374	8	67	theme	low	1498:1500	arg1	cost					1502:1505	low cost	1498:1505	low cost	1498:1505	All results showed the preparative adsorbents can be used as effective adsorbents for the treatment of waste water contaminated with RB49 since they are low cost and eco-friendly for the environment.
33600374	3	68	theme	X-ray	626:630	arg1	EDX					646:648	EDX	646:648	EDX	646:648	The two adsorbents were characterized for their quality by a scanning electron microscope (SEM), an energy-dispersive X-ray spectroscopy (EDX), and a Brunauer, Emmett and Teller (BET) analyzer.
33600374	3	68	theme	X-ray	626:630	arg1	spectroscopy					632:643	an energy-dispersive X-ray spectroscopy	605:643	an energy-dispersive X-ray spectroscopy (EDX)	605:649	The two adsorbents were characterized for their quality by a scanning electron microscope (SEM), an energy-dispersive X-ray spectroscopy (EDX), and a Brunauer, Emmett and Teller (BET) analyzer.
33600374	3	69	theme	scanning	569:576	arg1	SEM					599:601	SEM	599:601	SEM	599:601	The two adsorbents were characterized for their quality by a scanning electron microscope (SEM), an energy-dispersive X-ray spectroscopy (EDX), and a Brunauer, Emmett and Teller (BET) analyzer.
33600374	3	69	theme	scanning	569:576	arg1	microscope					587:596	a scanning electron microscope	567:596	a scanning electron microscope (SEM)	567:602	The two adsorbents were characterized for their quality by a scanning electron microscope (SEM), an energy-dispersive X-ray spectroscopy (EDX), and a Brunauer, Emmett and Teller (BET) analyzer.
33600374	7	70	theme	Freundlich	1137:1146	arg1	isotherm					1148:1155	The Freundlich isotherm	1133:1155	The Freundlich isotherm	1133:1155	The Freundlich isotherm was a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites, and the adsorption kinetics for both adsorbents were the pseudo-second-order rate equation.
33600374	7	70	theme	Freundlich	1137:1146	arg1	fit					1168:1170	a good fit	1161:1170	a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites	1161:1250	The Freundlich isotherm was a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites, and the adsorption kinetics for both adsorbents were the pseudo-second-order rate equation.
33600374	1	71	theme	chitosan/waste	148:161	arg1	C/WMS					177:181	Cross-linked chitosan/waste mussel shell (C/WMS)	135:182	Cross-linked chitosan/waste mussel shell (C/WMS)	135:182	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	5	72	theme	C/WMS/WASC	1005:1014	arg1	mg/g					1045:1048	54.7 and 38.8 mg/g	1031:1048	54.7 and 38.8 mg/g	1031:1048	The maximum RB49 adsorption capacity of C/WMS and C/WMS/WASC composites was 54.7 and 38.8 mg/g, respectively.
33600374	5	72	theme	C/WMS/WASC	1005:1014	arg1	capacity					983:990	The maximum RB49 adsorption capacity	955:990	The maximum RB49 adsorption capacity of C/WMS and C/WMS/WASC composites	955:1025	The maximum RB49 adsorption capacity of C/WMS and C/WMS/WASC composites was 54.7 and 38.8 mg/g, respectively.
33600374	8	73	theme	water	1454:1458	arg1	treatment					1435:1443	the treatment	1431:1443	the treatment of waste water contaminated with RB49	1431:1481	All results showed the preparative adsorbents can be used as effective adsorbents for the treatment of waste water contaminated with RB49 since they are low cost and eco-friendly for the environment.
33600374	8	74	dep	adsorbents	1380:1389	arg1	used					1398:1401	used	1398:1401	can be used as effective adsorbents for the treatment of waste water contaminated with RB49 since they are low cost and eco-friendly for the environment	1391:1542	All results showed the preparative adsorbents can be used as effective adsorbents for the treatment of waste water contaminated with RB49 since they are low cost and eco-friendly for the environment.
33600374	7	75	theme	pseudo-second-order	1310:1328	arg1	equation					1335:1342	the pseudo-second-order rate equation	1306:1342	the pseudo-second-order rate equation	1306:1342	The Freundlich isotherm was a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites, and the adsorption kinetics for both adsorbents were the pseudo-second-order rate equation.
33600374	7	75	theme	pseudo-second-order	1310:1328	arg1	kinetics					1272:1279	the adsorption kinetics	1257:1279	the adsorption kinetics for both adsorbents	1257:1299	The Freundlich isotherm was a good fit for the experimental data of RB49 adsorption on C/WMS and C/WMS/WASC composites, and the adsorption kinetics for both adsorbents were the pseudo-second-order rate equation.
33600374	3	76	theme	electron	578:585	arg1	SEM					599:601	SEM	599:601	SEM	599:601	The two adsorbents were characterized for their quality by a scanning electron microscope (SEM), an energy-dispersive X-ray spectroscopy (EDX), and a Brunauer, Emmett and Teller (BET) analyzer.
33600374	3	76	theme	electron	578:585	arg1	microscope					587:596	a scanning electron microscope	567:596	a scanning electron microscope (SEM)	567:602	The two adsorbents were characterized for their quality by a scanning electron microscope (SEM), an energy-dispersive X-ray spectroscopy (EDX), and a Brunauer, Emmett and Teller (BET) analyzer.
33600374	1	77	theme	mussel	163:168	arg1	C/WMS					177:181	Cross-linked chitosan/waste mussel shell (C/WMS)	135:182	Cross-linked chitosan/waste mussel shell (C/WMS)	135:182	Cross-linked chitosan/waste mussel shell (C/WMS) and chitosan/waste mussel shell/waste active sludge char (C/WMS/WASC) composites were prepared from waste mussel shell, waste active sludge (WAS), and chitosan, and cross-linked with glutaraldehyde.
33600374	4	78	theme	RB49	882:885	arg1	adsorption					888:897	Reactive Blue 49 (RB49) adsorption	864:897	Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites	864:934	The effects of contact time (0-1,620 mins), pH (1-5), adsorbent dosage (0.01-0.1 g/50 ml), initial dye concentration (20-100 mg/L), and temperature (25-45 °C) on Reactive Blue 49 (RB49) adsorption onto C/WMS and C/WMS/WASC composites were investigated.
33600374	8	79	theme	waste	1448:1452	arg1	water					1454:1458	waste water	1448:1458	waste water contaminated with RB49	1448:1481	All results showed the preparative adsorbents can be used as effective adsorbents for the treatment of waste water contaminated with RB49 since they are low cost and eco-friendly for the environment.
33212108	2	0	link	crosslinked	364:374	arg1	gels					445:448	chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels	355:448	chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels	355:448	In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin.
33212108	2	1	theme	dialdehyde	376:385	arg1	gum					395:397	dialdehyde xanthan gum	376:397	chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels	355:448	In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin.
33212108	1	2	theme	challenging	280:290	arg1	design					154:159	The design	150:159	The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers	150:273	The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers is a challenging task with vital implications in health sciences.
33212108	1	2	theme	challenging	280:290	arg1	task					292:295	a challenging task	278:295	a challenging task with vital implications in health sciences	278:338	The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers is a challenging task with vital implications in health sciences.
33212108	3	3	theme	rheology	627:634	arg1	method					636:641	rheology method	627:641	rheology method	627:641	The prepared hydrogel scaffolds were characterized by rheology method, FTIR, SEM, TGA and compression analysis.
33212108	0	4	link	Self-crosslinked	0:15	arg1	gum					45:47	Self-crosslinked chitosan/dialdehyde xanthan gum	0:47	Self-crosslinked chitosan/dialdehyde xanthan gum	0:47	Self-crosslinked chitosan/dialdehyde xanthan gum blended hypromellose hydrogel for the controlled delivery of ampicillin, minocycline and rifampicin.
33212108	1	5	theme	materials	201:209	arg1	design					154:159	The design	150:159	The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers	150:273	The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers is a challenging task with vital implications in health sciences.
33212108	1	5	theme	materials	201:209	arg1	task					292:295	a challenging task	278:295	a challenging task with vital implications in health sciences	278:338	The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers is a challenging task with vital implications in health sciences.
33212108	4	6	theme	drug	925:928	arg1	kinetics					938:945	affected drug release kinetics	916:945	affected drug release kinetics	916:945	In addition, gelation kinetics, swelling, in vitro degradation and drug release rate were studied under simulated gastrointestinal fluid conditions of pH 2.0 and 7.4 at 37 °C. Results demonstrated the gel composition and structure affected drug release kinetics.
33212108	4	7	theme	in	727:728	arg1	degradation					736:746	in vitro degradation	727:746	in vitro degradation	727:746	In addition, gelation kinetics, swelling, in vitro degradation and drug release rate were studied under simulated gastrointestinal fluid conditions of pH 2.0 and 7.4 at 37 °C. Results demonstrated the gel composition and structure affected drug release kinetics.
33212108	1	8	from	implications	308:319	arg1	sciences					331:338	health sciences	324:338	health sciences	324:338	The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers is a challenging task with vital implications in health sciences.
33212108	1	9	theme	high	216:219	arg1	load-capacity					221:233	high load-capacity	216:233	high load-capacity to serve as biocompatible drug carriers	216:273	The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers is a challenging task with vital implications in health sciences.
33212108	0	10	theme	rifampicin	138:147	arg1	delivery					98:105	the controlled delivery	83:105	the controlled delivery of ampicillin, minocycline and rifampicin	83:147	Self-crosslinked chitosan/dialdehyde xanthan gum blended hypromellose hydrogel for the controlled delivery of ampicillin, minocycline and rifampicin.
33212108	3	11	theme	prepared	577:584	arg1	scaffolds					595:603	The prepared hydrogel scaffolds	573:603	The prepared hydrogel scaffolds	573:603	The prepared hydrogel scaffolds were characterized by rheology method, FTIR, SEM, TGA and compression analysis.
33212108	8	12	theme	promising	1524:1532	arg1	material					1534:1541	promising material	1524:1541	promising material for controlled antibiotic delivery towards microbial growth inhibition	1524:1612	Overall, the present study demonstrated that the produced hydrogel scaffolds serves as promising material for controlled antibiotic delivery towards microbial growth inhibition.
33212108	8	13	theme	antibiotic	1558:1567	arg1	delivery					1569:1576	controlled antibiotic delivery	1547:1576	controlled antibiotic delivery towards microbial growth inhibition	1547:1612	Overall, the present study demonstrated that the produced hydrogel scaffolds serves as promising material for controlled antibiotic delivery towards microbial growth inhibition.
33212108	5	14	theme	cumulative	987:996	arg1	release					998:1004	more than 50% cumulative release	973:1004	more than 50% cumulative release	973:1004	The release study showed more than 50% cumulative release within 24 h for all investigated antibiotic drugs.
33212108	6	15	theme	mouse	1095:1099	arg1	lines					1127:1131	mouse embryonic fibroblast cell lines	1095:1131	mouse embryonic fibroblast cell lines	1095:1131	In vitro cell cytocompatibility using mouse embryonic fibroblast cell lines depicted ≥80% cell viability, indicating the gels are non-toxic.
33212108	7	16	theme	loaded	1237:1242	arg1	gels					1244:1247	loaded gels	1237:1247	loaded gels	1237:1247	Finally, the antibacterial activity of loaded gels was evaluated against Gram-negative and positive bacteria (Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia), which correlated well with swelling and drug release results.
33212108	1	17	theme	improved	164:171	arg1	materials					201:209	improved biopolymeric based hydrogel materials	164:209	improved biopolymeric based hydrogel materials	164:209	The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers is a challenging task with vital implications in health sciences.
33212108	8	18	theme	growth	1596:1601	arg1	inhibition					1603:1612	microbial growth inhibition	1586:1612	microbial growth inhibition	1586:1612	Overall, the present study demonstrated that the produced hydrogel scaffolds serves as promising material for controlled antibiotic delivery towards microbial growth inhibition.
33212108	4	19	theme	drug	752:755	arg1	rate					765:768	drug release rate	752:768	drug release rate	752:768	In addition, gelation kinetics, swelling, in vitro degradation and drug release rate were studied under simulated gastrointestinal fluid conditions of pH 2.0 and 7.4 at 37 °C. Results demonstrated the gel composition and structure affected drug release kinetics.
33212108	1	20	theme	based	186:190	arg1	materials					201:209	improved biopolymeric based hydrogel materials	164:209	improved biopolymeric based hydrogel materials	164:209	The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers is a challenging task with vital implications in health sciences.
33212108	5	21	dep	%	985:985	arg1	50					983:984	50	983:984	50	983:984	The release study showed more than 50% cumulative release within 24 h for all investigated antibiotic drugs.
33212108	8	22	theme	present	1450:1456	arg1	study					1458:1462	the present study	1446:1462	the present study	1446:1462	Overall, the present study demonstrated that the produced hydrogel scaffolds serves as promising material for controlled antibiotic delivery towards microbial growth inhibition.
33212108	0	23	theme	ampicillin	110:119	arg1	delivery					98:105	the controlled delivery	83:105	the controlled delivery of ampicillin, minocycline and rifampicin	83:147	Self-crosslinked chitosan/dialdehyde xanthan gum blended hypromellose hydrogel for the controlled delivery of ampicillin, minocycline and rifampicin.
33212108	5	24	theme	investigated	1026:1037	arg1	drugs					1050:1054	all investigated antibiotic drugs	1022:1054	all investigated antibiotic drugs	1022:1054	The release study showed more than 50% cumulative release within 24 h for all investigated antibiotic drugs.
33212108	8	25	dep	hydrogel	1495:1502	arg1	scaffolds					1504:1512	scaffolds	1504:1512	scaffolds	1504:1512	Overall, the present study demonstrated that the produced hydrogel scaffolds serves as promising material for controlled antibiotic delivery towards microbial growth inhibition.
33212108	0	26	theme	minocycline	122:132	arg1	delivery					98:105	the controlled delivery	83:105	the controlled delivery of ampicillin, minocycline and rifampicin	83:147	Self-crosslinked chitosan/dialdehyde xanthan gum blended hypromellose hydrogel for the controlled delivery of ampicillin, minocycline and rifampicin.
33212108	1	27	with	design	154:159	arg1	load-capacity					221:233	high load-capacity	216:233	high load-capacity to serve as biocompatible drug carriers	216:273	The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers is a challenging task with vital implications in health sciences.
33212108	7	28	theme	drug	1415:1418	arg1	release					1420:1426	drug release	1415:1426	drug release	1415:1426	Finally, the antibacterial activity of loaded gels was evaluated against Gram-negative and positive bacteria (Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia), which correlated well with swelling and drug release results.
33212108	2	29	theme	antibiotic	506:515	arg1	rifampicin					561:570	rifampicin	561:570	rifampicin	561:570	In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin.
33212108	2	29	theme	antibiotic	506:515	arg1	minocycline					545:555	minocycline	545:555	minocycline	545:555	In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin.
33212108	2	29	theme	antibiotic	506:515	arg1	ampicillin					533:542	ampicillin	533:542	ampicillin	533:542	In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin.
33212108	2	29	theme	antibiotic	506:515	arg1	drugs					517:521	different antibiotic drugs	496:521	different antibiotic drugs including ampicillin, minocycline and rifampicin	496:570	In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin.
33212108	4	30	theme	simulated	789:797	arg1	conditions					822:831	simulated gastrointestinal fluid conditions	789:831	simulated gastrointestinal fluid conditions of pH 2.0 and 7.4	789:849	In addition, gelation kinetics, swelling, in vitro degradation and drug release rate were studied under simulated gastrointestinal fluid conditions of pH 2.0 and 7.4 at 37 °C. Results demonstrated the gel composition and structure affected drug release kinetics.
33212108	2	31	theme	methylcellulose	429:443	arg1	gels					445:448	chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels	355:448	chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels	355:448	In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin.
33212108	0	32	theme	Self-crosslinked	0:15	arg1	gum					45:47	Self-crosslinked chitosan/dialdehyde xanthan gum	0:47	Self-crosslinked chitosan/dialdehyde xanthan gum	0:47	Self-crosslinked chitosan/dialdehyde xanthan gum blended hypromellose hydrogel for the controlled delivery of ampicillin, minocycline and rifampicin.
33212108	1	33	theme	vital	302:306	arg1	implications					308:319	vital implications	302:319	vital implications in health sciences	302:338	The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers is a challenging task with vital implications in health sciences.
33212108	6	34	theme	≥80	1142:1144	arg1	%					1145:1145	%	1145:1145	%	1145:1145	In vitro cell cytocompatibility using mouse embryonic fibroblast cell lines depicted ≥80% cell viability, indicating the gels are non-toxic.
33212108	4	35	theme	fluid	816:820	arg1	conditions					822:831	simulated gastrointestinal fluid conditions	789:831	simulated gastrointestinal fluid conditions of pH 2.0 and 7.4	789:849	In addition, gelation kinetics, swelling, in vitro degradation and drug release rate were studied under simulated gastrointestinal fluid conditions of pH 2.0 and 7.4 at 37 °C. Results demonstrated the gel composition and structure affected drug release kinetics.
33212108	2	36	theme	controlled	473:482	arg1	delivery					484:491	the controlled delivery	469:491	the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin	469:570	In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin.
33212108	0	37	theme	xanthan	37:43	arg1	gum					45:47	Self-crosslinked chitosan/dialdehyde xanthan gum	0:47	Self-crosslinked chitosan/dialdehyde xanthan gum	0:47	Self-crosslinked chitosan/dialdehyde xanthan gum blended hypromellose hydrogel for the controlled delivery of ampicillin, minocycline and rifampicin.
33212108	3	38	dep	method	636:641	arg1	analysis					675:682	analysis	675:682	analysis	675:682	The prepared hydrogel scaffolds were characterized by rheology method, FTIR, SEM, TGA and compression analysis.
33212108	7	39	dep	swelling	1402:1409	arg1	results					1428:1434	results	1428:1434	results	1428:1434	Finally, the antibacterial activity of loaded gels was evaluated against Gram-negative and positive bacteria (Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia), which correlated well with swelling and drug release results.
33212108	1	40	with	task	292:295	arg1	implications					308:319	vital implications	302:319	vital implications in health sciences	302:338	The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers is a challenging task with vital implications in health sciences.
33212108	6	41	theme	fibroblast	1111:1120	arg1	lines					1127:1131	mouse embryonic fibroblast cell lines	1095:1131	mouse embryonic fibroblast cell lines	1095:1131	In vitro cell cytocompatibility using mouse embryonic fibroblast cell lines depicted ≥80% cell viability, indicating the gels are non-toxic.
33212108	8	42	theme	produced	1486:1493	arg1	hydrogel					1495:1502	the produced hydrogel scaffolds	1482:1512	the produced hydrogel scaffolds	1482:1512	Overall, the present study demonstrated that the produced hydrogel scaffolds serves as promising material for controlled antibiotic delivery towards microbial growth inhibition.
33212108	2	43	theme	crosslinked	364:374	arg1	gels					445:448	chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels	355:448	chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels	355:448	In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin.
33212108	1	44	theme	biocompatible	247:259	arg1	carriers					266:273	biocompatible drug carriers	247:273	biocompatible drug carriers	247:273	The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers is a challenging task with vital implications in health sciences.
33212108	3	45	theme	hydrogel	586:593	arg1	scaffolds					595:603	The prepared hydrogel scaffolds	573:603	The prepared hydrogel scaffolds	573:603	The prepared hydrogel scaffolds were characterized by rheology method, FTIR, SEM, TGA and compression analysis.
33212108	4	46	dep	37 °C.	854:859	arg1	demonstrated					869:880	demonstrated	869:880	demonstrated the gel composition and structure affected drug release kinetics	869:945	In addition, gelation kinetics, swelling, in vitro degradation and drug release rate were studied under simulated gastrointestinal fluid conditions of pH 2.0 and 7.4 at 37 °C. Results demonstrated the gel composition and structure affected drug release kinetics.
33212108	2	47	theme	xanthan	387:393	arg1	gum					395:397	dialdehyde xanthan gum	376:397	chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels	355:448	In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin.
33212108	4	48	theme	release	930:936	arg1	kinetics					938:945	affected drug release kinetics	916:945	affected drug release kinetics	916:945	In addition, gelation kinetics, swelling, in vitro degradation and drug release rate were studied under simulated gastrointestinal fluid conditions of pH 2.0 and 7.4 at 37 °C. Results demonstrated the gel composition and structure affected drug release kinetics.
33212108	5	49	dep	release	998:1004	arg1	%					985:985	%	985:985	%	985:985	The release study showed more than 50% cumulative release within 24 h for all investigated antibiotic drugs.
33212108	1	50	theme	hydrogel	192:199	arg1	materials					201:209	improved biopolymeric based hydrogel materials	164:209	improved biopolymeric based hydrogel materials	164:209	The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers is a challenging task with vital implications in health sciences.
33212108	4	51	theme	affected	916:923	arg1	kinetics					938:945	affected drug release kinetics	916:945	affected drug release kinetics	916:945	In addition, gelation kinetics, swelling, in vitro degradation and drug release rate were studied under simulated gastrointestinal fluid conditions of pH 2.0 and 7.4 at 37 °C. Results demonstrated the gel composition and structure affected drug release kinetics.
33212108	4	52	dep	composition	890:900	arg1	kinetics					938:945	affected drug release kinetics	916:945	affected drug release kinetics	916:945	In addition, gelation kinetics, swelling, in vitro degradation and drug release rate were studied under simulated gastrointestinal fluid conditions of pH 2.0 and 7.4 at 37 °C. Results demonstrated the gel composition and structure affected drug release kinetics.
33212108	8	53	theme	controlled	1547:1556	arg1	delivery					1569:1576	controlled antibiotic delivery	1547:1576	controlled antibiotic delivery towards microbial growth inhibition	1547:1612	Overall, the present study demonstrated that the produced hydrogel scaffolds serves as promising material for controlled antibiotic delivery towards microbial growth inhibition.
33212108	4	54	theme	gelation	698:705	arg1	kinetics					707:714	gelation kinetics	698:714	gelation kinetics	698:714	In addition, gelation kinetics, swelling, in vitro degradation and drug release rate were studied under simulated gastrointestinal fluid conditions of pH 2.0 and 7.4 at 37 °C. Results demonstrated the gel composition and structure affected drug release kinetics.
33212108	4	55	theme	release	757:763	arg1	rate					765:768	drug release rate	752:768	drug release rate	752:768	In addition, gelation kinetics, swelling, in vitro degradation and drug release rate were studied under simulated gastrointestinal fluid conditions of pH 2.0 and 7.4 at 37 °C. Results demonstrated the gel composition and structure affected drug release kinetics.
33212108	0	56	theme	hypromellose	57:68	arg1	hydrogel					70:77	hypromellose hydrogel	57:77	hypromellose hydrogel	57:77	Self-crosslinked chitosan/dialdehyde xanthan gum blended hypromellose hydrogel for the controlled delivery of ampicillin, minocycline and rifampicin.
33212108	6	57	theme	embryonic	1101:1109	arg1	lines					1127:1131	mouse embryonic fibroblast cell lines	1095:1131	mouse embryonic fibroblast cell lines	1095:1131	In vitro cell cytocompatibility using mouse embryonic fibroblast cell lines depicted ≥80% cell viability, indicating the gels are non-toxic.
33212108	8	58	theme	microbial	1586:1594	arg1	inhibition					1603:1612	microbial growth inhibition	1586:1612	microbial growth inhibition	1586:1612	Overall, the present study demonstrated that the produced hydrogel scaffolds serves as promising material for controlled antibiotic delivery towards microbial growth inhibition.
33212108	0	59	theme	controlled	87:96	arg1	delivery					98:105	the controlled delivery	83:105	the controlled delivery of ampicillin, minocycline and rifampicin	83:147	Self-crosslinked chitosan/dialdehyde xanthan gum blended hypromellose hydrogel for the controlled delivery of ampicillin, minocycline and rifampicin.
33212108	5	60	theme	release	952:958	arg1	study					960:964	The release study	948:964	The release study	948:964	The release study showed more than 50% cumulative release within 24 h for all investigated antibiotic drugs.
33212108	6	61	dep	In	1057:1058	arg1	vitro					1060:1064	vitro	1060:1064	vitro	1060:1064	In vitro cell cytocompatibility using mouse embryonic fibroblast cell lines depicted ≥80% cell viability, indicating the gels are non-toxic.
33212108	7	62	theme	positive	1289:1296	arg1	bacteria					1298:1305	Gram-negative and positive bacteria	1271:1305	Gram-negative and positive bacteria (Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia)	1271:1372	Finally, the antibacterial activity of loaded gels was evaluated against Gram-negative and positive bacteria (Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia), which correlated well with swelling and drug release results.
33212108	1	63	theme	biopolymeric	173:184	arg1	materials					201:209	improved biopolymeric based hydrogel materials	164:209	improved biopolymeric based hydrogel materials	164:209	The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers is a challenging task with vital implications in health sciences.
33212108	6	64	theme	cell	1066:1069	arg1	cytocompatibility					1071:1087	In vitro cell cytocompatibility	1057:1087	In vitro cell cytocompatibility using mouse embryonic fibroblast cell lines	1057:1131	In vitro cell cytocompatibility using mouse embryonic fibroblast cell lines depicted ≥80% cell viability, indicating the gels are non-toxic.
33212108	7	65	theme	Gram-negative	1271:1283	arg1	bacteria					1298:1305	Gram-negative and positive bacteria	1271:1305	Gram-negative and positive bacteria (Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia)	1271:1372	Finally, the antibacterial activity of loaded gels was evaluated against Gram-negative and positive bacteria (Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia), which correlated well with swelling and drug release results.
33212108	6	66	theme	In	1057:1058	arg1	cytocompatibility					1071:1087	In vitro cell cytocompatibility	1057:1087	In vitro cell cytocompatibility using mouse embryonic fibroblast cell lines	1057:1131	In vitro cell cytocompatibility using mouse embryonic fibroblast cell lines depicted ≥80% cell viability, indicating the gels are non-toxic.
33212108	5	67	theme	antibiotic	1039:1048	arg1	drugs					1050:1054	all investigated antibiotic drugs	1022:1054	all investigated antibiotic drugs	1022:1054	The release study showed more than 50% cumulative release within 24 h for all investigated antibiotic drugs.
33212108	4	68	theme	pH 2.0	836:841	arg1	conditions					822:831	simulated gastrointestinal fluid conditions	789:831	simulated gastrointestinal fluid conditions of pH 2.0 and 7.4	789:849	In addition, gelation kinetics, swelling, in vitro degradation and drug release rate were studied under simulated gastrointestinal fluid conditions of pH 2.0 and 7.4 at 37 °C. Results demonstrated the gel composition and structure affected drug release kinetics.
33212108	0	69	theme	chitosan/dialdehyde	17:35	arg1	gum					45:47	Self-crosslinked chitosan/dialdehyde xanthan gum	0:47	Self-crosslinked chitosan/dialdehyde xanthan gum	0:47	Self-crosslinked chitosan/dialdehyde xanthan gum blended hypromellose hydrogel for the controlled delivery of ampicillin, minocycline and rifampicin.
33212108	6	70	theme	%	1145:1145	arg1	viability					1152:1160	≥80% cell viability	1142:1160	≥80% cell viability	1142:1160	In vitro cell cytocompatibility using mouse embryonic fibroblast cell lines depicted ≥80% cell viability, indicating the gels are non-toxic.
33212108	2	71	theme	different	496:504	arg1	rifampicin					561:570	rifampicin	561:570	rifampicin	561:570	In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin.
33212108	2	71	theme	different	496:504	arg1	minocycline					545:555	minocycline	545:555	minocycline	545:555	In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin.
33212108	2	71	theme	different	496:504	arg1	ampicillin					533:542	ampicillin	533:542	ampicillin	533:542	In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin.
33212108	2	71	theme	different	496:504	arg1	drugs					517:521	different antibiotic drugs	496:521	different antibiotic drugs including ampicillin, minocycline and rifampicin	496:570	In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin.
33212108	2	72	theme	hydroxypropyl	415:427	arg1	gels					445:448	chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels	355:448	chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels	355:448	In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin.
33212108	7	73	dep	bacteria	1298:1305	arg1	coli					1320:1323	Escherichia coli	1308:1323	Escherichia coli	1308:1323	Finally, the antibacterial activity of loaded gels was evaluated against Gram-negative and positive bacteria (Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia), which correlated well with swelling and drug release results.
33212108	7	73	dep	bacteria	1298:1305	arg1	aureus					1341:1346	Staphylococcus aureus	1326:1346	Staphylococcus aureus	1326:1346	Finally, the antibacterial activity of loaded gels was evaluated against Gram-negative and positive bacteria (Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia), which correlated well with swelling and drug release results.
33212108	7	73	dep	bacteria	1298:1305	arg1	pneumonia					1363:1371	Klebsiella pneumonia	1352:1371	Klebsiella pneumonia	1352:1371	Finally, the antibacterial activity of loaded gels was evaluated against Gram-negative and positive bacteria (Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia), which correlated well with swelling and drug release results.
33212108	4	74	theme	gastrointestinal	799:814	arg1	conditions					822:831	simulated gastrointestinal fluid conditions	789:831	simulated gastrointestinal fluid conditions of pH 2.0 and 7.4	789:849	In addition, gelation kinetics, swelling, in vitro degradation and drug release rate were studied under simulated gastrointestinal fluid conditions of pH 2.0 and 7.4 at 37 °C. Results demonstrated the gel composition and structure affected drug release kinetics.
33212108	2	75	theme	interpenetrated	399:413	arg1	gels					445:448	chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels	355:448	chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels	355:448	In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin.
33212108	7	76	theme	antibacterial	1211:1223	arg1	activity					1225:1232	the antibacterial activity	1207:1232	the antibacterial activity of loaded gels	1207:1247	Finally, the antibacterial activity of loaded gels was evaluated against Gram-negative and positive bacteria (Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia), which correlated well with swelling and drug release results.
33212108	1	77	theme	health	324:329	arg1	sciences					331:338	health sciences	324:338	health sciences	324:338	The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers is a challenging task with vital implications in health sciences.
33212108	6	78	theme	cell	1122:1125	arg1	lines					1127:1131	mouse embryonic fibroblast cell lines	1095:1131	mouse embryonic fibroblast cell lines	1095:1131	In vitro cell cytocompatibility using mouse embryonic fibroblast cell lines depicted ≥80% cell viability, indicating the gels are non-toxic.
33212108	2	79	theme	drugs	517:521	arg1	delivery					484:491	the controlled delivery	469:491	the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin	469:570	In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin.
33212108	4	80	theme	gel	886:888	arg1	composition					890:900	the gel composition	882:900	the gel composition	882:900	In addition, gelation kinetics, swelling, in vitro degradation and drug release rate were studied under simulated gastrointestinal fluid conditions of pH 2.0 and 7.4 at 37 °C. Results demonstrated the gel composition and structure affected drug release kinetics.
33212108	2	81	theme	chitosan	355:362	arg1	gels					445:448	chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels	355:448	chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels	355:448	In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin.
33212108	4	82	dep	in	727:728	arg1	vitro					730:734	vitro	730:734	vitro	730:734	In addition, gelation kinetics, swelling, in vitro degradation and drug release rate were studied under simulated gastrointestinal fluid conditions of pH 2.0 and 7.4 at 37 °C. Results demonstrated the gel composition and structure affected drug release kinetics.
33212108	4	83	theme	7.4	847:849	arg1	conditions					822:831	simulated gastrointestinal fluid conditions	789:831	simulated gastrointestinal fluid conditions of pH 2.0 and 7.4	789:849	In addition, gelation kinetics, swelling, in vitro degradation and drug release rate were studied under simulated gastrointestinal fluid conditions of pH 2.0 and 7.4 at 37 °C. Results demonstrated the gel composition and structure affected drug release kinetics.
33212108	2	84	theme	gum	395:397	arg1	gels					445:448	chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels	355:448	chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels	355:448	In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin.
33212108	1	85	theme	drug	261:264	arg1	carriers					266:273	biocompatible drug carriers	247:273	biocompatible drug carriers	247:273	The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers is a challenging task with vital implications in health sciences.
33212108	6	86	theme	cell	1147:1150	arg1	viability					1152:1160	≥80% cell viability	1142:1160	≥80% cell viability	1142:1160	In vitro cell cytocompatibility using mouse embryonic fibroblast cell lines depicted ≥80% cell viability, indicating the gels are non-toxic.
33212108	7	87	theme	gels	1244:1247	arg1	activity					1225:1232	the antibacterial activity	1207:1232	the antibacterial activity of loaded gels	1207:1247	Finally, the antibacterial activity of loaded gels was evaluated against Gram-negative and positive bacteria (Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia), which correlated well with swelling and drug release results.
32006575	4	0	theme	1.0:0.26:0.08	831:843	arg1	ratio					822:826	a molar ratio	814:826	a molar ratio of 1.0:0.26:0.08	814:843	The monosaccharide composition of EPS was mannose, arabinose and glucose at a molar ratio of 1.0:0.26:0.08.
32006575	0	1	theme	properties	173:182	arg1	Study					125:129	Study	125:129	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.	0:253	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	0	2	theme	application	188:198	arg1	Study					125:129	Study	125:129	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.	0:253	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	3	3	theme	%	696:696	arg1	w/v					698:700	0.75% w/v	692:700	0.75% w/v	692:700	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	3	3	theme	%	696:696	arg1	peptone					683:689	peptone	683:689	peptone (0.75% w/v)	683:701	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	3	4	theme	TS/P	566:569	arg1	ratio					571:575	TS/P ratio	566:575	TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1	566:623	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	9	5	theme	production	1380:1389	arg1	enhancement					1348:1358	an enhancement	1345:1358	an enhancement of not only biomass production of S. platensis, but also cellular contents (pigments, proteins and lipids) leading to higher nutritional value	1345:1501	It induced an enhancement of not only biomass production of S. platensis, but also cellular contents (pigments, proteins and lipids) leading to higher nutritional value.
32006575	3	6	theme	viscosity	600:608	arg1	ratio					571:575	TS/P ratio	566:575	TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1	566:623	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	9	7	theme	S.	1394:1395	arg1	platensis					1397:1405	S. platensis	1394:1405	S. platensis	1394:1405	It induced an enhancement of not only biomass production of S. platensis, but also cellular contents (pigments, proteins and lipids) leading to higher nutritional value.
32006575	3	8	theme	incubation	707:716	arg1	period					718:723	incubation period	707:723	incubation period of 4.69 day	707:735	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	3	9	theme	g-1	621:623	arg1	ratio					571:575	TS/P ratio	566:575	TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1	566:623	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	3	10	with	production	536:545	arg1	ratio					571:575	TS/P ratio	566:575	TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1	566:623	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	0	11	theme	potential	138:146	arg1	antioxidant					148:158	its potential antioxidant	134:158	its potential antioxidant	134:158	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	9	12	theme	contents	1426:1433	arg1	enhancement					1348:1358	an enhancement	1345:1358	an enhancement of not only biomass production of S. platensis, but also cellular contents (pigments, proteins and lipids) leading to higher nutritional value	1345:1501	It induced an enhancement of not only biomass production of S. platensis, but also cellular contents (pigments, proteins and lipids) leading to higher nutritional value.
32006575	3	13	theme	optimal	641:647	arg1	conditions					649:658	optimal conditions	641:658	optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day	641:735	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	7	14	from	strength	1166:1173	arg1	activity					1106:1113	The emulsifying activity	1090:1113	The emulsifying activity	1090:1113	The emulsifying activity was stable at different temperatures, pH and ionic strength.
32006575	7	14	from	strength	1166:1173	arg1	stable					1119:1124	stable	1119:1124	stable	1119:1124	The emulsifying activity was stable at different temperatures, pH and ionic strength.
32006575	8	15	theme	carbon	1241:1246	arg1	hydrolysate					1199:1209	the acid hydrolysate	1190:1209	the acid hydrolysate of the EPS	1190:1220	Additionally, the acid hydrolysate of the EPS was evaluated as a carbon source for the mixotrophic cultivation of industrially important Spirulina platensis.
32006575	8	15	theme	carbon	1241:1246	arg1	source					1248:1253	a carbon source	1239:1253	a carbon source for the mixotrophic cultivation of industrially important Spirulina platensis	1239:1331	Additionally, the acid hydrolysate of the EPS was evaluated as a carbon source for the mixotrophic cultivation of industrially important Spirulina platensis.
32006575	0	16	theme	exopolysaccharide	57:73	arg1	production					16:25	production	16:25	production	16:25	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	0	16	theme	exopolysaccharide	57:73	arg1	viscosity					41:49	intrinsic viscosity	31:49	intrinsic viscosity	31:49	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	0	17	from	production	16:25	arg1	high					82:85	high	82:85	high	82:85	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	0	18	theme	platensis	244:252	arg1	cultivation					219:229	the mixotrophic cultivation	203:229	the mixotrophic cultivation of Spirulina platensis	203:252	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	5	19	theme	water	866:870	arg1	solubility					872:881	high water solubility	861:881	high water solubility (38.5%)	861:889	The EPS showed high water solubility (38.5%), water holding capacity (514.46%) and foaming capacity (55.55%).
32006575	5	19	theme	water	866:870	arg1	%					888:888	38.5%	884:888	38.5%	884:888	The EPS showed high water solubility (38.5%), water holding capacity (514.46%) and foaming capacity (55.55%).
32006575	8	20	theme	mixotrophic	1263:1273	arg1	cultivation					1275:1285	the mixotrophic cultivation	1259:1285	the mixotrophic cultivation of industrially important Spirulina platensis	1259:1331	Additionally, the acid hydrolysate of the EPS was evaluated as a carbon source for the mixotrophic cultivation of industrially important Spirulina platensis.
32006575	4	21	from	ratio	822:826	arg1	arabinose					789:797	arabinose	789:797	arabinose	789:797	The monosaccharide composition of EPS was mannose, arabinose and glucose at a molar ratio of 1.0:0.26:0.08.
32006575	4	21	from	ratio	822:826	arg1	composition					757:767	The monosaccharide composition	738:767	The monosaccharide composition of EPS	738:774	The monosaccharide composition of EPS was mannose, arabinose and glucose at a molar ratio of 1.0:0.26:0.08.
32006575	4	21	from	ratio	822:826	arg1	mannose					780:786	mannose	780:786	mannose	780:786	The monosaccharide composition of EPS was mannose, arabinose and glucose at a molar ratio of 1.0:0.26:0.08.
32006575	4	21	from	ratio	822:826	arg1	glucose					803:809	glucose	803:809	glucose	803:809	The monosaccharide composition of EPS was mannose, arabinose and glucose at a molar ratio of 1.0:0.26:0.08.
32006575	7	22	theme	different	1129:1137	arg1	temperatures					1139:1150	different temperatures	1129:1150	different temperatures	1129:1150	The emulsifying activity was stable at different temperatures, pH and ionic strength.
32006575	9	23	theme	higher	1478:1483	arg1	value					1497:1501	higher nutritional value	1478:1501	higher nutritional value	1478:1501	It induced an enhancement of not only biomass production of S. platensis, but also cellular contents (pigments, proteins and lipids) leading to higher nutritional value.
32006575	8	24	theme	important	1303:1311	arg1	platensis					1323:1331	industrially important Spirulina platensis	1290:1331	industrially important Spirulina platensis	1290:1331	Additionally, the acid hydrolysate of the EPS was evaluated as a carbon source for the mixotrophic cultivation of industrially important Spirulina platensis.
32006575	6	25	theme	moderate	971:978	arg1	activity					992:999	moderate antioxidant activity	971:999	moderate antioxidant activity in vitro	971:1008	The EPS showed moderate antioxidant activity in vitro and good emulsion stabilizing properties against several hydrophobic compounds.
32006575	8	26	theme	platensis	1323:1331	arg1	cultivation					1275:1285	the mixotrophic cultivation	1259:1285	the mixotrophic cultivation of industrially important Spirulina platensis	1259:1331	Additionally, the acid hydrolysate of the EPS was evaluated as a carbon source for the mixotrophic cultivation of industrially important Spirulina platensis.
32006575	0	27	theme	production	16:25	arg1	Optimization					0:11	Optimization	0:11	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.	0:253	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	5	28	theme	holding	898:904	arg1	capacity					906:913	water holding capacity	892:913	water holding capacity (514.46%)	892:923	The EPS showed high water solubility (38.5%), water holding capacity (514.46%) and foaming capacity (55.55%).
32006575	5	28	theme	holding	898:904	arg1	%					922:922	514.46%	916:922	514.46%	916:922	The EPS showed high water solubility (38.5%), water holding capacity (514.46%) and foaming capacity (55.55%).
32006575	2	29	theme	Box-Behnken	491:501	arg1	design					516:521	Box-Behnken experimental design	491:521	Box-Behnken experimental design	491:521	The EPS production and its physico-chemical properties (intrinsic viscosity and total sugars/protein (TS/P) ratio) were optimized using Box-Behnken experimental design.
32006575	2	30	theme	total	435:439	arg1	ratio					463:467	total sugars/protein (TS/P) ratio	435:467	total sugars/protein (TS/P) ratio	435:467	The EPS production and its physico-chemical properties (intrinsic viscosity and total sugars/protein (TS/P) ratio) were optimized using Box-Behnken experimental design.
32006575	0	31	theme	intrinsic	31:39	arg1	viscosity					41:49	intrinsic viscosity	31:49	intrinsic viscosity	31:49	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	2	32	theme	EPS	359:361	arg1	production					363:372	EPS production	359:372	EPS production	359:372	The EPS production and its physico-chemical properties (intrinsic viscosity and total sugars/protein (TS/P) ratio) were optimized using Box-Behnken experimental design.
32006575	4	33	theme	EPS	772:774	arg1	mannose					780:786	mannose	780:786	mannose	780:786	The monosaccharide composition of EPS was mannose, arabinose and glucose at a molar ratio of 1.0:0.26:0.08.
32006575	4	33	theme	EPS	772:774	arg1	composition					757:767	The monosaccharide composition	738:767	The monosaccharide composition of EPS	738:774	The monosaccharide composition of EPS was mannose, arabinose and glucose at a molar ratio of 1.0:0.26:0.08.
32006575	6	34	theme	stabilizing	1028:1038	arg1	properties					1040:1049	good emulsion stabilizing properties	1014:1049	good emulsion stabilizing properties	1014:1049	The EPS showed moderate antioxidant activity in vitro and good emulsion stabilizing properties against several hydrophobic compounds.
32006575	3	35	theme	%	675:675	arg1	sucrose					663:669	sucrose	663:669	sucrose (4.0% w/v)	663:680	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	3	35	theme	%	675:675	arg1	w/v					677:679	4.0% w/v	672:679	4.0% w/v	672:679	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	2	36	theme	physico-chemical	382:397	arg1	properties					399:408	its physico-chemical properties	378:408	its physico-chemical properties	378:408	The EPS production and its physico-chemical properties (intrinsic viscosity and total sugars/protein (TS/P) ratio) were optimized using Box-Behnken experimental design.
32006575	0	37	dep	Optimization	0:11	arg1	Study					125:129	Study	125:129	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.	0:253	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	6	38	theme	good	1014:1017	arg1	properties					1040:1049	good emulsion stabilizing properties	1014:1049	good emulsion stabilizing properties	1014:1049	The EPS showed moderate antioxidant activity in vitro and good emulsion stabilizing properties against several hydrophobic compounds.
32006575	3	39	theme	EPS	532:534	arg1	production					536:545	Maximum EPS production	524:545	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1	524:623	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	1	40	theme	salarius	269:276	arg1	BM02					278:281	Virgibacillus salarius BM02	255:281	Virgibacillus salarius BM02	255:281	Virgibacillus salarius BM02 was identified as a highly exopolysaccharide (EPS) producing bacterium.
32006575	1	40	theme	salarius	269:276	arg1	exopolysaccharide					310:326	a highly exopolysaccharide	301:326	a highly exopolysaccharide (EPS) producing bacterium	301:352	Virgibacillus salarius BM02 was identified as a highly exopolysaccharide (EPS) producing bacterium.
32006575	3	41	theme	peptone	683:689	arg1	conditions					649:658	optimal conditions	641:658	optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day	641:735	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	0	42	theme	mixotrophic	207:217	arg1	cultivation					219:229	the mixotrophic cultivation	203:229	the mixotrophic cultivation of Spirulina platensis	203:252	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	9	43	theme	biomass	1372:1378	arg1	production					1380:1389	biomass production	1372:1389	biomass production of S. platensis	1372:1405	It induced an enhancement of not only biomass production of S. platensis, but also cellular contents (pigments, proteins and lipids) leading to higher nutritional value.
32006575	3	44	theme	intrinsic	590:598	arg1	viscosity					600:608	intrinsic viscosity	590:608	intrinsic viscosity	590:608	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	4	45	theme	molar	816:820	arg1	ratio					822:826	a molar ratio	814:826	a molar ratio of 1.0:0.26:0.08	814:843	The monosaccharide composition of EPS was mannose, arabinose and glucose at a molar ratio of 1.0:0.26:0.08.
32006575	3	46	theme	4.69 day	728:735	arg1	sucrose					663:669	sucrose	663:669	sucrose (4.0% w/v)	663:680	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	3	46	theme	4.69 day	728:735	arg1	w/v					698:700	0.75% w/v	692:700	0.75% w/v	692:700	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	3	46	theme	4.69 day	728:735	arg1	w/v					677:679	4.0% w/v	672:679	4.0% w/v	672:679	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	3	46	theme	4.69 day	728:735	arg1	peptone					683:689	peptone	683:689	peptone (0.75% w/v)	683:701	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	3	46	theme	4.69 day	728:735	arg1	period					718:723	incubation period	707:723	incubation period of 4.69 day	707:735	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	3	47	theme	12.56	580:584	arg1	ratio					571:575	TS/P ratio	566:575	TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1	566:623	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	9	48	theme	platensis	1397:1405	arg1	production					1380:1389	biomass production	1372:1389	biomass production of S. platensis	1372:1405	It induced an enhancement of not only biomass production of S. platensis, but also cellular contents (pigments, proteins and lipids) leading to higher nutritional value.
32006575	9	48	theme	platensis	1397:1405	arg1	contents					1426:1433	cellular contents	1417:1433	cellular contents (pigments, proteins and lipids)	1417:1465	It induced an enhancement of not only biomass production of S. platensis, but also cellular contents (pigments, proteins and lipids) leading to higher nutritional value.
32006575	0	49	from	high	82:85	arg1	production					16:25	production	16:25	production	16:25	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	0	49	from	high	82:85	arg1	viscosity					41:49	intrinsic viscosity	31:49	intrinsic viscosity	31:49	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	0	49	from	high	82:85	arg1	exopolysaccharide					57:73	an exopolysaccharide	54:73	an exopolysaccharide from a high yielding Virgibacillus salarius BM02	54:122	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	6	50	theme	hydrophobic	1067:1077	arg1	compounds					1079:1087	several hydrophobic compounds	1059:1087	several hydrophobic compounds	1059:1087	The EPS showed moderate antioxidant activity in vitro and good emulsion stabilizing properties against several hydrophobic compounds.
32006575	8	51	theme	acid	1194:1197	arg1	hydrolysate					1199:1209	the acid hydrolysate	1190:1209	the acid hydrolysate of the EPS	1190:1220	Additionally, the acid hydrolysate of the EPS was evaluated as a carbon source for the mixotrophic cultivation of industrially important Spirulina platensis.
32006575	8	51	theme	acid	1194:1197	arg1	source					1248:1253	a carbon source	1239:1253	a carbon source for the mixotrophic cultivation of industrially important Spirulina platensis	1239:1331	Additionally, the acid hydrolysate of the EPS was evaluated as a carbon source for the mixotrophic cultivation of industrially important Spirulina platensis.
32006575	3	52	theme	period	718:723	arg1	conditions					649:658	optimal conditions	641:658	optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day	641:735	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	3	53	theme	dL	618:619	arg1	g-1					621:623	0.13 dL g-1	613:623	0.13 dL g-1	613:623	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	7	54	from	temperatures	1139:1150	arg1	activity					1106:1113	The emulsifying activity	1090:1113	The emulsifying activity	1090:1113	The emulsifying activity was stable at different temperatures, pH and ionic strength.
32006575	7	54	from	temperatures	1139:1150	arg1	stable					1119:1124	stable	1119:1124	stable	1119:1124	The emulsifying activity was stable at different temperatures, pH and ionic strength.
32006575	0	55	theme	antioxidant	148:158	arg1	Study					125:129	Study	125:129	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.	0:253	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	9	56	theme	cellular	1417:1424	arg1	contents					1426:1433	cellular contents	1417:1433	cellular contents (pigments, proteins and lipids)	1417:1465	It induced an enhancement of not only biomass production of S. platensis, but also cellular contents (pigments, proteins and lipids) leading to higher nutritional value.
32006575	7	57	from	pH	1153:1154	arg1	activity					1106:1113	The emulsifying activity	1090:1113	The emulsifying activity	1090:1113	The emulsifying activity was stable at different temperatures, pH and ionic strength.
32006575	7	57	from	pH	1153:1154	arg1	stable					1119:1124	stable	1119:1124	stable	1119:1124	The emulsifying activity was stable at different temperatures, pH and ionic strength.
32006575	8	58	theme	EPS	1218:1220	arg1	hydrolysate					1199:1209	the acid hydrolysate	1190:1209	the acid hydrolysate of the EPS	1190:1220	Additionally, the acid hydrolysate of the EPS was evaluated as a carbon source for the mixotrophic cultivation of industrially important Spirulina platensis.
32006575	8	58	theme	EPS	1218:1220	arg1	source					1248:1253	a carbon source	1239:1253	a carbon source for the mixotrophic cultivation of industrially important Spirulina platensis	1239:1331	Additionally, the acid hydrolysate of the EPS was evaluated as a carbon source for the mixotrophic cultivation of industrially important Spirulina platensis.
32006575	0	59	theme	emulsifying	161:171	arg1	properties					173:182	emulsifying properties	161:182	emulsifying properties	161:182	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	7	60	theme	ionic	1160:1164	arg1	strength					1166:1173	ionic strength	1160:1173	ionic strength	1160:1173	The emulsifying activity was stable at different temperatures, pH and ionic strength.
32006575	5	61	theme	high	861:864	arg1	solubility					872:881	high water solubility	861:881	high water solubility (38.5%)	861:889	The EPS showed high water solubility (38.5%), water holding capacity (514.46%) and foaming capacity (55.55%).
32006575	5	61	theme	high	861:864	arg1	%					888:888	38.5%	884:888	38.5%	884:888	The EPS showed high water solubility (38.5%), water holding capacity (514.46%) and foaming capacity (55.55%).
32006575	2	62	theme	experimental	503:514	arg1	design					516:521	Box-Behnken experimental design	491:521	Box-Behnken experimental design	491:521	The EPS production and its physico-chemical properties (intrinsic viscosity and total sugars/protein (TS/P) ratio) were optimized using Box-Behnken experimental design.
32006575	0	63	theme	Virgibacillus	96:108	arg1	BM02					119:122	Virgibacillus salarius BM02	96:122	Virgibacillus salarius BM02	96:122	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	0	64	theme	Spirulina	234:242	arg1	platensis					244:252	Spirulina platensis	234:252	Spirulina platensis	234:252	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	2	65	dep	production	363:372	arg1	The					355:357	The	355:357	The	355:357	The EPS production and its physico-chemical properties (intrinsic viscosity and total sugars/protein (TS/P) ratio) were optimized using Box-Behnken experimental design.
32006575	2	65	dep	production	363:372	arg1	ratio					463:467	total sugars/protein (TS/P) ratio	435:467	total sugars/protein (TS/P) ratio	435:467	The EPS production and its physico-chemical properties (intrinsic viscosity and total sugars/protein (TS/P) ratio) were optimized using Box-Behnken experimental design.
32006575	2	65	dep	production	363:372	arg1	viscosity					421:429	intrinsic viscosity	411:429	intrinsic viscosity	411:429	The EPS production and its physico-chemical properties (intrinsic viscosity and total sugars/protein (TS/P) ratio) were optimized using Box-Behnken experimental design.
32006575	0	66	dep	Virgibacillus	96:108	arg1	salarius					110:117	salarius	110:117	salarius	110:117	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	0	67	from	viscosity	41:49	arg1	high					82:85	high	82:85	high	82:85	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	9	68	dep	contents	1426:1433	arg1	proteins					1446:1453	proteins	1446:1453	proteins	1446:1453	It induced an enhancement of not only biomass production of S. platensis, but also cellular contents (pigments, proteins and lipids) leading to higher nutritional value.
32006575	9	68	dep	contents	1426:1433	arg1	pigments					1436:1443	pigments	1436:1443	pigments	1436:1443	It induced an enhancement of not only biomass production of S. platensis, but also cellular contents (pigments, proteins and lipids) leading to higher nutritional value.
32006575	9	68	dep	contents	1426:1433	arg1	lipids					1459:1464	lipids	1459:1464	lipids	1459:1464	It induced an enhancement of not only biomass production of S. platensis, but also cellular contents (pigments, proteins and lipids) leading to higher nutritional value.
32006575	2	69	theme	intrinsic	411:419	arg1	viscosity					421:429	intrinsic viscosity	411:429	intrinsic viscosity	411:429	The EPS production and its physico-chemical properties (intrinsic viscosity and total sugars/protein (TS/P) ratio) were optimized using Box-Behnken experimental design.
32006575	9	70	theme	nutritional	1485:1495	arg1	value					1497:1501	higher nutritional value	1478:1501	higher nutritional value	1478:1501	It induced an enhancement of not only biomass production of S. platensis, but also cellular contents (pigments, proteins and lipids) leading to higher nutritional value.
32006575	6	71	theme	antioxidant	980:990	arg1	activity					992:999	moderate antioxidant activity	971:999	moderate antioxidant activity in vitro	971:1008	The EPS showed moderate antioxidant activity in vitro and good emulsion stabilizing properties against several hydrophobic compounds.
32006575	7	72	theme	emulsifying	1094:1104	arg1	activity					1106:1113	The emulsifying activity	1090:1113	The emulsifying activity	1090:1113	The emulsifying activity was stable at different temperatures, pH and ionic strength.
32006575	7	72	theme	emulsifying	1094:1104	arg1	stable					1119:1124	stable	1119:1124	stable	1119:1124	The emulsifying activity was stable at different temperatures, pH and ionic strength.
32006575	5	73	theme	water	892:896	arg1	capacity					906:913	water holding capacity	892:913	water holding capacity (514.46%)	892:923	The EPS showed high water solubility (38.5%), water holding capacity (514.46%) and foaming capacity (55.55%).
32006575	5	73	theme	water	892:896	arg1	%					922:922	514.46%	916:922	514.46%	916:922	The EPS showed high water solubility (38.5%), water holding capacity (514.46%) and foaming capacity (55.55%).
32006575	3	74	theme	sucrose	663:669	arg1	conditions					649:658	optimal conditions	641:658	optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day	641:735	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	8	75	theme	Spirulina	1313:1321	arg1	platensis					1323:1331	industrially important Spirulina platensis	1290:1331	industrially important Spirulina platensis	1290:1331	Additionally, the acid hydrolysate of the EPS was evaluated as a carbon source for the mixotrophic cultivation of industrially important Spirulina platensis.
32006575	2	76	theme	sugars/protein	441:454	arg1	ratio					463:467	total sugars/protein (TS/P) ratio	435:467	total sugars/protein (TS/P) ratio	435:467	The EPS production and its physico-chemical properties (intrinsic viscosity and total sugars/protein (TS/P) ratio) were optimized using Box-Behnken experimental design.
32006575	0	77	theme	viscosity	41:49	arg1	Optimization					0:11	Optimization	0:11	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.	0:253	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	6	78	theme	several	1059:1065	arg1	compounds					1079:1087	several hydrophobic compounds	1059:1087	several hydrophobic compounds	1059:1087	The EPS showed moderate antioxidant activity in vitro and good emulsion stabilizing properties against several hydrophobic compounds.
32006575	3	79	theme	Maximum	524:530	arg1	production					536:545	Maximum EPS production	524:545	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1	524:623	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	4	80	theme	monosaccharide	742:755	arg1	mannose					780:786	mannose	780:786	mannose	780:786	The monosaccharide composition of EPS was mannose, arabinose and glucose at a molar ratio of 1.0:0.26:0.08.
32006575	4	80	theme	monosaccharide	742:755	arg1	composition					757:767	The monosaccharide composition	738:767	The monosaccharide composition of EPS	738:774	The monosaccharide composition of EPS was mannose, arabinose and glucose at a molar ratio of 1.0:0.26:0.08.
32006575	5	81	theme	foaming	929:935	arg1	capacity					937:944	foaming capacity	929:944	foaming capacity (55.55%)	929:953	The EPS showed high water solubility (38.5%), water holding capacity (514.46%) and foaming capacity (55.55%).
32006575	5	81	theme	foaming	929:935	arg1	%					952:952	55.55%	947:952	55.55%	947:952	The EPS showed high water solubility (38.5%), water holding capacity (514.46%) and foaming capacity (55.55%).
32006575	6	82	theme	emulsion	1019:1026	arg1	properties					1040:1049	good emulsion stabilizing properties	1014:1049	good emulsion stabilizing properties	1014:1049	The EPS showed moderate antioxidant activity in vitro and good emulsion stabilizing properties against several hydrophobic compounds.
32006575	0	83	from	Study	125:129	arg1	cultivation					219:229	the mixotrophic cultivation	203:229	the mixotrophic cultivation of Spirulina platensis	203:252	Optimization of production and intrinsic viscosity of an exopolysaccharide from a high yielding Virgibacillus salarius BM02: Study of its potential antioxidant, emulsifying properties and application in the mixotrophic cultivation of Spirulina platensis.
32006575	3	84	theme	5.87 g L-1	550:559	arg1	production					536:545	Maximum EPS production	524:545	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1	524:623	Maximum EPS production of 5.87 g L-1 with TS/P ratio of 12.56 and intrinsic viscosity of 0.13 dL g-1 was obtained at optimal conditions of sucrose (4.0% w/v), peptone (0.75% w/v) and incubation period of 4.69 day.
32006575	1	85	theme	Virgibacillus	255:267	arg1	BM02					278:281	Virgibacillus salarius BM02	255:281	Virgibacillus salarius BM02	255:281	Virgibacillus salarius BM02 was identified as a highly exopolysaccharide (EPS) producing bacterium.
32006575	1	85	theme	Virgibacillus	255:267	arg1	exopolysaccharide					310:326	a highly exopolysaccharide	301:326	a highly exopolysaccharide (EPS) producing bacterium	301:352	Virgibacillus salarius BM02 was identified as a highly exopolysaccharide (EPS) producing bacterium.
32531909	2	0	theme	scaffolds	365:373	arg1	Nowadays					298:305	Nowadays	298:305	Nowadays	298:305	Nowadays, the metallization of naturally predesigned, 3D chitinous scaffolds originating from marine sponges is drawing focused attention.
32531909	2	0	theme	scaffolds	365:373	arg1	metallization					312:324	the metallization	308:324	the metallization of naturally predesigned, 3D chitinous scaffolds originating from marine sponges	308:405	Nowadays, the metallization of naturally predesigned, 3D chitinous scaffolds originating from marine sponges is drawing focused attention.
32531909	0	1	theme	Silver	76:81	arg1	Nanoparticles					83:95	Silver Nanoparticles	76:95	Silver Nanoparticles	76:95	Functionalization of 3D Chitinous Skeletal Scaffolds of Sponge Origin Using Silver Nanoparticles and Their Antibacterial Properties.
32531909	2	2	theme	chitinous	355:363	arg1	scaffolds					365:373	naturally predesigned, 3D chitinous scaffolds	329:373	naturally predesigned, 3D chitinous scaffolds originating from marine sponges	329:405	Nowadays, the metallization of naturally predesigned, 3D chitinous scaffolds originating from marine sponges is drawing focused attention.
32531909	3	3	theme	farming	558:564	arg1	conditions					566:575	marine farming conditions	551:575	marine farming conditions	551:575	These invertebrates represent a unique, renewable source of specialized chitin due to their ability to grow under marine farming conditions.
32531909	4	4	from	development	597:607	arg1	form					638:641	the form	634:641	the form of 3D chitin-based skeletal scaffolds covered with silver nanoparticles (AgNPs) and Ag-bromide	634:736	In this study, the development of composite material in the form of 3D chitin-based skeletal scaffolds covered with silver nanoparticles (AgNPs) and Ag-bromide is described for the first time.
32531909	5	5	theme	filtration	883:892	arg1	system					894:899	a water filtration system	875:899	a water filtration system	875:899	Additionally, the antibacterial properties of the obtained materials and their possible applications as a water filtration system are also investigated.
32531909	4	6	theme	scaffolds	671:679	arg1	form					638:641	the form	634:641	the form of 3D chitin-based skeletal scaffolds covered with silver nanoparticles (AgNPs) and Ag-bromide	634:736	In this study, the development of composite material in the form of 3D chitin-based skeletal scaffolds covered with silver nanoparticles (AgNPs) and Ag-bromide is described for the first time.
32531909	0	7	theme	Antibacterial	107:119	arg1	Properties					121:130	Their Antibacterial Properties	101:130	Their Antibacterial Properties	101:130	Functionalization of 3D Chitinous Skeletal Scaffolds of Sponge Origin Using Silver Nanoparticles and Their Antibacterial Properties.
32531909	3	8	theme	marine	551:556	arg1	conditions					566:575	marine farming conditions	551:575	marine farming conditions	551:575	These invertebrates represent a unique, renewable source of specialized chitin due to their ability to grow under marine farming conditions.
32531909	1	9	theme	abundant	165:172	arg1	polysaccharides					185:199	nature's most abundant structural polysaccharides	151:199	nature's most abundant structural polysaccharides	151:199	Chitin, as one of nature's most abundant structural polysaccharides, possesses worldwide, high industrial potential and a functionality that is topically pertinent.
32531909	4	10	theme	skeletal	662:669	arg1	scaffolds					671:679	3D chitin-based skeletal scaffolds	646:679	3D chitin-based skeletal scaffolds covered with silver nanoparticles (AgNPs) and Ag-bromide	646:736	In this study, the development of composite material in the form of 3D chitin-based skeletal scaffolds covered with silver nanoparticles (AgNPs) and Ag-bromide is described for the first time.
32531909	2	11	dep	predesigned	339:349	arg1	3D					352:353	3D	352:353	3D	352:353	Nowadays, the metallization of naturally predesigned, 3D chitinous scaffolds originating from marine sponges is drawing focused attention.
32531909	1	12	theme	structural	174:183	arg1	polysaccharides					185:199	nature's most abundant structural polysaccharides	151:199	nature's most abundant structural polysaccharides	151:199	Chitin, as one of nature's most abundant structural polysaccharides, possesses worldwide, high industrial potential and a functionality that is topically pertinent.
32531909	5	13	theme	obtained	821:828	arg1	materials					830:838	the obtained materials	817:838	the obtained materials	817:838	Additionally, the antibacterial properties of the obtained materials and their possible applications as a water filtration system are also investigated.
32531909	5	14	theme	water	877:881	arg1	system					894:899	a water filtration system	875:899	a water filtration system	875:899	Additionally, the antibacterial properties of the obtained materials and their possible applications as a water filtration system are also investigated.
32531909	2	15	theme	marine	392:397	arg1	sponges					399:405	marine sponges	392:405	marine sponges	392:405	Nowadays, the metallization of naturally predesigned, 3D chitinous scaffolds originating from marine sponges is drawing focused attention.
32531909	0	16	theme	Chitinous	24:32	arg1	Scaffolds					43:51	3D Chitinous Skeletal Scaffolds	21:51	3D Chitinous Skeletal Scaffolds of Sponge Origin Using Silver Nanoparticles and Their Antibacterial Properties	21:130	Functionalization of 3D Chitinous Skeletal Scaffolds of Sponge Origin Using Silver Nanoparticles and Their Antibacterial Properties.
32531909	4	17	theme	first	759:763	arg1	time					765:768	the first time	755:768	the first time	755:768	In this study, the development of composite material in the form of 3D chitin-based skeletal scaffolds covered with silver nanoparticles (AgNPs) and Ag-bromide is described for the first time.
32531909	0	18	theme	3D	21:22	arg1	Scaffolds					43:51	3D Chitinous Skeletal Scaffolds	21:51	3D Chitinous Skeletal Scaffolds of Sponge Origin Using Silver Nanoparticles and Their Antibacterial Properties	21:130	Functionalization of 3D Chitinous Skeletal Scaffolds of Sponge Origin Using Silver Nanoparticles and Their Antibacterial Properties.
32531909	5	19	theme	possible	850:857	arg1	applications					859:870	their possible applications	844:870	their possible applications as a water filtration system	844:899	Additionally, the antibacterial properties of the obtained materials and their possible applications as a water filtration system are also investigated.
32531909	0	20	theme	Scaffolds	43:51	arg1	Functionalization					0:16	Functionalization	0:16	Functionalization of 3D Chitinous Skeletal Scaffolds of Sponge Origin Using Silver Nanoparticles and Their Antibacterial Properties.	0:131	Functionalization of 3D Chitinous Skeletal Scaffolds of Sponge Origin Using Silver Nanoparticles and Their Antibacterial Properties.
32531909	4	21	theme	chitin-based	649:660	arg1	scaffolds					671:679	3D chitin-based skeletal scaffolds	646:679	3D chitin-based skeletal scaffolds covered with silver nanoparticles (AgNPs) and Ag-bromide	646:736	In this study, the development of composite material in the form of 3D chitin-based skeletal scaffolds covered with silver nanoparticles (AgNPs) and Ag-bromide is described for the first time.
32531909	0	22	theme	Skeletal	34:41	arg1	Scaffolds					43:51	3D Chitinous Skeletal Scaffolds	21:51	3D Chitinous Skeletal Scaffolds of Sponge Origin Using Silver Nanoparticles and Their Antibacterial Properties	21:130	Functionalization of 3D Chitinous Skeletal Scaffolds of Sponge Origin Using Silver Nanoparticles and Their Antibacterial Properties.
32531909	5	23	theme	antibacterial	789:801	arg1	properties					803:812	the antibacterial properties	785:812	the antibacterial properties of the obtained materials	785:838	Additionally, the antibacterial properties of the obtained materials and their possible applications as a water filtration system are also investigated.
32531909	5	24	theme	materials	830:838	arg1	properties					803:812	the antibacterial properties	785:812	the antibacterial properties of the obtained materials	785:838	Additionally, the antibacterial properties of the obtained materials and their possible applications as a water filtration system are also investigated.
32531909	5	24	theme	materials	830:838	arg1	applications					859:870	their possible applications	844:870	their possible applications as a water filtration system	844:899	Additionally, the antibacterial properties of the obtained materials and their possible applications as a water filtration system are also investigated.
32531909	3	25	theme	renewable	477:485	arg1	source					487:492	a unique, renewable source	467:492	source	487:492	These invertebrates represent a unique, renewable source of specialized chitin due to their ability to grow under marine farming conditions.
32531909	0	26	theme	Sponge	56:61	arg1	Origin					63:68	Sponge Origin	56:68	Sponge Origin	56:68	Functionalization of 3D Chitinous Skeletal Scaffolds of Sponge Origin Using Silver Nanoparticles and Their Antibacterial Properties.
32531909	4	27	theme	composite	612:620	arg1	material					622:629	composite material	612:629	composite material	612:629	In this study, the development of composite material in the form of 3D chitin-based skeletal scaffolds covered with silver nanoparticles (AgNPs) and Ag-bromide is described for the first time.
32531909	3	28	theme	specialized	497:507	arg1	chitin					509:514	specialized chitin	497:514	specialized chitin	497:514	These invertebrates represent a unique, renewable source of specialized chitin due to their ability to grow under marine farming conditions.
32531909	4	29	theme	silver	694:699	arg1	AgNPs					716:720	AgNPs	716:720	AgNPs	716:720	In this study, the development of composite material in the form of 3D chitin-based skeletal scaffolds covered with silver nanoparticles (AgNPs) and Ag-bromide is described for the first time.
32531909	4	29	theme	silver	694:699	arg1	nanoparticles					701:713	silver nanoparticles	694:713	silver nanoparticles (AgNPs)	694:721	In this study, the development of composite material in the form of 3D chitin-based skeletal scaffolds covered with silver nanoparticles (AgNPs) and Ag-bromide is described for the first time.
32531909	2	30	theme	predesigned	339:349	arg1	scaffolds					365:373	naturally predesigned, 3D chitinous scaffolds	329:373	naturally predesigned, 3D chitinous scaffolds originating from marine sponges	329:405	Nowadays, the metallization of naturally predesigned, 3D chitinous scaffolds originating from marine sponges is drawing focused attention.
32531909	1	31	theme	high	223:226	arg1	potential					239:247	high industrial potential	223:247	high industrial potential	223:247	Chitin, as one of nature's most abundant structural polysaccharides, possesses worldwide, high industrial potential and a functionality that is topically pertinent.
32531909	1	32	contain	possesses	202:210	arg2	functionality					255:267	a functionality	253:267	a functionality	253:267	Chitin, as one of nature's most abundant structural polysaccharides, possesses worldwide, high industrial potential and a functionality that is topically pertinent.
32531909	1	32	contain	possesses	202:210	arg2	worldwide					212:220	worldwide	212:220	worldwide	212:220	Chitin, as one of nature's most abundant structural polysaccharides, possesses worldwide, high industrial potential and a functionality that is topically pertinent.
32531909	1	32	contain	possesses	202:210	arg1	one					144:146	one	144:146	one	144:146	Chitin, as one of nature's most abundant structural polysaccharides, possesses worldwide, high industrial potential and a functionality that is topically pertinent.
32531909	1	32	contain	possesses	202:210	arg1	Chitin					133:138	Chitin	133:138	Chitin	133:138	Chitin, as one of nature's most abundant structural polysaccharides, possesses worldwide, high industrial potential and a functionality that is topically pertinent.
32531909	1	32	contain	possesses	202:210	arg2	pertinent					287:295	pertinent	287:295	pertinent	287:295	Chitin, as one of nature's most abundant structural polysaccharides, possesses worldwide, high industrial potential and a functionality that is topically pertinent.
32531909	1	32	contain	possesses	202:210	arg2	potential					239:247	high industrial potential	223:247	high industrial potential	223:247	Chitin, as one of nature's most abundant structural polysaccharides, possesses worldwide, high industrial potential and a functionality that is topically pertinent.
32531909	3	33	theme	chitin	509:514	arg1	source					487:492	a unique, renewable source	467:492	source	487:492	These invertebrates represent a unique, renewable source of specialized chitin due to their ability to grow under marine farming conditions.
32531909	1	34	theme	industrial	228:237	arg1	potential					239:247	high industrial potential	223:247	high industrial potential	223:247	Chitin, as one of nature's most abundant structural polysaccharides, possesses worldwide, high industrial potential and a functionality that is topically pertinent.
32531909	4	35	theme	3D	646:647	arg1	scaffolds					671:679	3D chitin-based skeletal scaffolds	646:679	3D chitin-based skeletal scaffolds covered with silver nanoparticles (AgNPs) and Ag-bromide	646:736	In this study, the development of composite material in the form of 3D chitin-based skeletal scaffolds covered with silver nanoparticles (AgNPs) and Ag-bromide is described for the first time.
32531909	2	36	dep	drawing	410:416	arg1	focused					418:424	focused	418:424	is drawing focused attention	407:434	Nowadays, the metallization of naturally predesigned, 3D chitinous scaffolds originating from marine sponges is drawing focused attention.
32531909	3	37	theme	unique	469:474	arg1	source					487:492	a unique, renewable source	467:492	source	487:492	These invertebrates represent a unique, renewable source of specialized chitin due to their ability to grow under marine farming conditions.
32531909	0	38	theme	Origin	63:68	arg1	Scaffolds					43:51	3D Chitinous Skeletal Scaffolds	21:51	3D Chitinous Skeletal Scaffolds of Sponge Origin Using Silver Nanoparticles and Their Antibacterial Properties	21:130	Functionalization of 3D Chitinous Skeletal Scaffolds of Sponge Origin Using Silver Nanoparticles and Their Antibacterial Properties.
32531909	4	39	theme	material	622:629	arg1	development					597:607	the development	593:607	the development of composite material in the form of 3D chitin-based skeletal scaffolds covered with silver nanoparticles (AgNPs) and Ag-bromide	593:736	In this study, the development of composite material in the form of 3D chitin-based skeletal scaffolds covered with silver nanoparticles (AgNPs) and Ag-bromide is described for the first time.
33125195	4	0	theme	95	1021:1022	arg1	%					1023:1023	%	1023:1023	%	1023:1023	MATERIAL AND METHODS The starch-clay composites were prepared by heating a dispersion of the starch in distilled water, then precipitating the dispersion with an equal volume of 95% ethanol.
33125195	2	1	theme	Schott	562:567	arg1	tubers					569:574	Colocasia esculenta L. Schott tubers	539:574	Colocasia esculenta L. Schott tubers	539:574	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	4	2	theme	ethanol	1025:1031	arg1	volume					1011:1016	an equal volume	1002:1016	an equal volume of 95% ethanol	1002:1031	MATERIAL AND METHODS The starch-clay composites were prepared by heating a dispersion of the starch in distilled water, then precipitating the dispersion with an equal volume of 95% ethanol.
33125195	1	3	from	excipients	307:316	arg1	formulations					328:339	tablet formulations	321:339	tablet formulations	321:339	BACKGROUND Co-processing starch with clay nanocomposite has been shown to yield a new class of materials, potentially with better properties than pristine starch, that could be used as directly compressible excipients in tablet formulations.
33125195	2	4	theme	montmorillonite	600:614	arg1	clay					616:619	montmorillonite clay	600:619	montmorillonite clay	600:619	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	2	5	from	OBJECTIVES	342:351	arg1	study					361:365	this study	356:365	this study	356:365	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	1	6	theme	new	182:184	arg1	class					186:190	a new class	180:190	a new class of materials	180:203	BACKGROUND Co-processing starch with clay nanocomposite has been shown to yield a new class of materials, potentially with better properties than pristine starch, that could be used as directly compressible excipients in tablet formulations.
33125195	2	7	theme	Colocasia	539:547	arg1	tubers					569:574	Colocasia esculenta L. Schott tubers	539:574	Colocasia esculenta L. Schott tubers	539:574	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	11	8	theme	tramadol	1882:1889	arg1	release					1871:1877	the controlled release	1856:1877	the controlled release of tramadol	1856:1889	CONCLUSIONS The study showed that starch-clay biocomposites could be used in the controlled release of tramadol.
33125195	2	9	with	co-processed	582:593	arg1	clay					616:619	montmorillonite clay	600:619	montmorillonite clay	600:619	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	2	10	theme	cocoyam	519:525	arg1	starch					527:532	cocoyam starch	519:532	cocoyam starch from Colocasia esculenta L. Schott tubers	519:574	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	2	11	from	grains	454:459	arg1	starch					416:421	millet starch	409:421	millet starch from Pennistum glaucum (L) RBr grains	409:459	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	2	11	from	grains	454:459	arg1	starch					470:475	sorghum starch	462:475	sorghum starch from Sorghum bicolor L. Moench grains	462:513	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	2	11	from	grains	454:459	arg1	starch					527:532	cocoyam starch	519:532	cocoyam starch from Colocasia esculenta L. Schott tubers	519:574	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	10	12	theme	starch-clay	1698:1708	arg1	composites					1710:1719	the starch-clay composites	1694:1719	the starch-clay composites	1694:1719	The properties of the starch-clay composites were not related to the botanical source of the starches.
33125195	9	13	theme	drug	1526:1529	arg1	properties					1539:1548	The mechanical and drug release properties	1507:1548	properties	1539:1548	The mechanical and drug release properties of tramadol tablets containing starch-clay composites were significantly better than those containing only pristine starches.
33125195	2	14	theme	esculenta	549:557	arg1	tubers					569:574	Colocasia esculenta L. Schott tubers	539:574	Colocasia esculenta L. Schott tubers	539:574	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	3	15	theme	starch-to-clay	736:749	arg1	ratios					751:756	different starch-to-clay ratios	726:756	different starch-to-clay ratios	726:756	The effects of different starch-to-clay ratios on the material and drug release properties of the resulting tablets were evaluated.
33125195	1	16	theme	clay	137:140	arg1	nanocomposite					142:154	clay nanocomposite	137:154	clay nanocomposite	137:154	BACKGROUND Co-processing starch with clay nanocomposite has been shown to yield a new class of materials, potentially with better properties than pristine starch, that could be used as directly compressible excipients in tablet formulations.
33125195	7	17	dep	RESULTS	1237:1243	arg1	yielded					1281:1287	yielded	1281:1287	yielded starch-clay composites with different material and tablet properties than the pristine starches	1281:1383	RESULTS Co-processing MMT with the starches yielded starch-clay composites with different material and tablet properties than the pristine starches.
33125195	3	18	theme	drug	778:781	arg1	properties					791:800	the material and drug release properties	761:800	properties	791:800	The effects of different starch-to-clay ratios on the material and drug release properties of the resulting tablets were evaluated.
33125195	9	19	theme	tramadol	1553:1560	arg1	tablets					1562:1568	tramadol tablets	1553:1568	tramadol tablets containing starch-clay composites	1553:1602	The mechanical and drug release properties of tramadol tablets containing starch-clay composites were significantly better than those containing only pristine starches.
33125195	3	20	theme	tablets	819:825	arg1	material					765:772	material	765:772	material	765:772	The effects of different starch-to-clay ratios on the material and drug release properties of the resulting tablets were evaluated.
33125195	6	21	theme	drug	1182:1185	arg1	properties					1195:1204	The mechanical and drug release properties	1163:1204	properties	1195:1204	The mechanical and drug release properties of the tablets were evaluated.
33125195	11	22	theme	starch-clay	1813:1823	arg1	biocomposites					1825:1837	starch-clay biocomposites	1813:1837	starch-clay biocomposites	1813:1837	CONCLUSIONS The study showed that starch-clay biocomposites could be used in the controlled release of tramadol.
33125195	5	23	theme	direct	1092:1097	arg1	composites					1050:1059	The starch-clay composites	1034:1059	The starch-clay composites	1034:1059	The starch-clay composites were characterized and used as direct compression excipients for the preparation of tramadol tablets.
33125195	5	23	theme	direct	1092:1097	arg1	excipients					1111:1120	direct compression excipients	1092:1120	direct compression excipients for the preparation of tramadol tablets	1092:1160	The starch-clay composites were characterized and used as direct compression excipients for the preparation of tramadol tablets.
33125195	2	24	theme	tramadol	681:688	arg1	formulations					697:708	tramadol tablet formulations	681:708	tramadol tablet formulations	681:708	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	0	25	theme	tablet	79:84	arg1	formulations					86:97	tramadol tablet formulations	70:97	tramadol tablet formulations	70:97	Evaluation of starch-clay composites as a pharmaceutical excipient in tramadol tablet formulations.
33125195	2	26	theme	L.	498:499	arg1	grains					508:513	Sorghum bicolor L. Moench grains	482:513	Sorghum bicolor L. Moench grains	482:513	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	6	27	theme	release	1187:1193	arg1	properties					1195:1204	The mechanical and drug release properties	1163:1204	properties	1195:1204	The mechanical and drug release properties of the tablets were evaluated.
33125195	2	28	theme	Sorghum	482:488	arg1	grains					508:513	Sorghum bicolor L. Moench grains	482:513	Sorghum bicolor L. Moench grains	482:513	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	2	29	dep	starch	416:421	arg1	i.e.					403:406	i.e.	403:406	i.e.	403:406	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	3	30	theme	release	783:789	arg1	properties					791:800	the material and drug release properties	761:800	properties	791:800	The effects of different starch-to-clay ratios on the material and drug release properties of the resulting tablets were evaluated.
33125195	11	31	theme	controlled	1860:1869	arg1	release					1871:1877	the controlled release	1856:1877	the controlled release of tramadol	1856:1889	CONCLUSIONS The study showed that starch-clay biocomposites could be used in the controlled release of tramadol.
33125195	7	32	theme	tablet	1340:1345	arg1	properties					1347:1356	different material and tablet properties	1317:1356	different material and tablet properties	1317:1356	RESULTS Co-processing MMT with the starches yielded starch-clay composites with different material and tablet properties than the pristine starches.
33125195	2	33	theme	glaucum	438:444	arg1	grains					454:459	Pennistum glaucum (L) RBr grains	428:459	Pennistum glaucum (L) RBr grains	428:459	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	5	34	theme	tablets	1154:1160	arg1	preparation					1130:1140	the preparation	1126:1140	the preparation of tramadol tablets	1126:1160	The starch-clay composites were characterized and used as direct compression excipients for the preparation of tramadol tablets.
33125195	1	35	theme	BACKGROUND	100:109	arg1	starch					125:130	BACKGROUND Co-processing starch	100:130	BACKGROUND Co-processing starch with clay nanocomposite	100:154	BACKGROUND Co-processing starch with clay nanocomposite has been shown to yield a new class of materials, potentially with better properties than pristine starch, that could be used as directly compressible excipients in tablet formulations.
33125195	7	36	theme	material	1327:1334	arg1	properties					1347:1356	different material and tablet properties	1317:1356	different material and tablet properties	1317:1356	RESULTS Co-processing MMT with the starches yielded starch-clay composites with different material and tablet properties than the pristine starches.
33125195	8	37	theme	co-processed	1390:1401	arg1	biocomposites					1414:1426	The co-processed starch-MMT biocomposites	1386:1426	The co-processed starch-MMT biocomposites	1386:1426	The co-processed starch-MMT biocomposites exhibited improved flowability and compressibility over the pristine starches.
33125195	2	38	theme	millet	409:414	arg1	starch					416:421	millet starch	409:421	millet starch from Pennistum glaucum (L) RBr grains	409:459	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	0	39	theme	starch-clay	14:24	arg1	composites					26:35	starch-clay composites	14:35	starch-clay composites	14:35	Evaluation of starch-clay composites as a pharmaceutical excipient in tramadol tablet formulations.
33125195	9	40	theme	pristine	1657:1664	arg1	starches					1666:1673	only pristine starches	1652:1673	only pristine starches	1652:1673	The mechanical and drug release properties of tramadol tablets containing starch-clay composites were significantly better than those containing only pristine starches.
33125195	2	41	theme	RBr	450:452	arg1	grains					454:459	Pennistum glaucum (L) RBr grains	428:459	Pennistum glaucum (L) RBr grains	428:459	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	2	42	from	tubers	569:574	arg1	starch					416:421	millet starch	409:421	millet starch from Pennistum glaucum (L) RBr grains	409:459	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	2	42	from	tubers	569:574	arg1	starch					470:475	sorghum starch	462:475	sorghum starch from Sorghum bicolor L. Moench grains	462:513	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	2	42	from	tubers	569:574	arg1	starch					527:532	cocoyam starch	519:532	cocoyam starch from Colocasia esculenta L. Schott tubers	519:574	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	10	43	theme	botanical	1745:1753	arg1	source					1755:1760	the botanical source	1741:1760	the botanical source of the starches	1741:1776	The properties of the starch-clay composites were not related to the botanical source of the starches.
33125195	1	44	theme	compressible	294:305	arg1	excipients					307:316	directly compressible excipients	285:316	directly compressible excipients in tablet formulations	285:339	BACKGROUND Co-processing starch with clay nanocomposite has been shown to yield a new class of materials, potentially with better properties than pristine starch, that could be used as directly compressible excipients in tablet formulations.
33125195	1	44	theme	compressible	294:305	arg1	starch					255:260	pristine starch	246:260	pristine starch	246:260	BACKGROUND Co-processing starch with clay nanocomposite has been shown to yield a new class of materials, potentially with better properties than pristine starch, that could be used as directly compressible excipients in tablet formulations.
33125195	7	45	theme	starch-clay	1289:1299	arg1	composites					1301:1310	starch-clay composites	1289:1310	starch-clay composites with different material and tablet properties	1289:1356	RESULTS Co-processing MMT with the starches yielded starch-clay composites with different material and tablet properties than the pristine starches.
33125195	2	46	theme	sorghum	462:468	arg1	starch					470:475	sorghum starch	462:475	sorghum starch from Sorghum bicolor L. Moench grains	462:513	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	8	47	theme	improved	1438:1445	arg1	flowability					1447:1457	improved flowability	1438:1457	improved flowability	1438:1457	The co-processed starch-MMT biocomposites exhibited improved flowability and compressibility over the pristine starches.
33125195	0	48	theme	pharmaceutical	42:55	arg1	excipient					57:65	a pharmaceutical excipient	40:65	a pharmaceutical excipient in tramadol tablet formulations	40:97	Evaluation of starch-clay composites as a pharmaceutical excipient in tramadol tablet formulations.
33125195	11	49	used	used	1848:1851	arg2	biocomposites					1825:1837	starch-clay biocomposites	1813:1837	starch-clay biocomposites	1813:1837	CONCLUSIONS The study showed that starch-clay biocomposites could be used in the controlled release of tramadol.
33125195	2	50	from	sources	394:400	arg1	OBJECTIVES					342:351	OBJECTIVES	342:351	OBJECTIVES In this study	342:365	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	2	50	from	sources	394:400	arg1	starches					368:375	starches	368:375	starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers	368:574	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	9	51	contain	containing	1570:1579	arg2	composites					1593:1602	starch-clay composites	1581:1602	starch-clay composites	1581:1602	The mechanical and drug release properties of tramadol tablets containing starch-clay composites were significantly better than those containing only pristine starches.
33125195	9	51	contain	containing	1570:1579	arg1	tablets					1562:1568	tramadol tablets	1553:1568	tramadol tablets containing starch-clay composites	1553:1602	The mechanical and drug release properties of tramadol tablets containing starch-clay composites were significantly better than those containing only pristine starches.
33125195	10	52	theme	starches	1769:1776	arg1	source					1755:1760	the botanical source	1741:1760	the botanical source of the starches	1741:1776	The properties of the starch-clay composites were not related to the botanical source of the starches.
33125195	1	53	theme	better	223:228	arg1	properties					230:239	better properties	223:239	better properties than pristine starch, that could be used as directly compressible excipients in tablet formulations	223:339	BACKGROUND Co-processing starch with clay nanocomposite has been shown to yield a new class of materials, potentially with better properties than pristine starch, that could be used as directly compressible excipients in tablet formulations.
33125195	7	54	theme	Co-processing	1245:1257	arg1	MMT					1259:1261	Co-processing MMT	1245:1261	Co-processing MMT with the starches	1245:1279	RESULTS Co-processing MMT with the starches yielded starch-clay composites with different material and tablet properties than the pristine starches.
33125195	4	55	theme	%	1023:1023	arg1	ethanol					1025:1031	95% ethanol	1021:1031	95% ethanol	1021:1031	MATERIAL AND METHODS The starch-clay composites were prepared by heating a dispersion of the starch in distilled water, then precipitating the dispersion with an equal volume of 95% ethanol.
33125195	2	56	theme	L.	559:560	arg1	tubers					569:574	Colocasia esculenta L. Schott tubers	539:574	Colocasia esculenta L. Schott tubers	539:574	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	9	57	contain	containing	1641:1650	arg2	starches					1666:1673	only pristine starches	1652:1673	only pristine starches	1652:1673	The mechanical and drug release properties of tramadol tablets containing starch-clay composites were significantly better than those containing only pristine starches.
33125195	9	57	contain	containing	1641:1650	arg1	those					1635:1639	those	1635:1639	those	1635:1639	The mechanical and drug release properties of tramadol tablets containing starch-clay composites were significantly better than those containing only pristine starches.
33125195	7	58	with	MMT	1259:1261	arg1	starches					1272:1279	the starches	1268:1279	the starches	1268:1279	RESULTS Co-processing MMT with the starches yielded starch-clay composites with different material and tablet properties than the pristine starches.
33125195	4	59	dep	MATERIAL	843:850	arg1	composites					880:889	The starch-clay composites	864:889	The starch-clay composites	864:889	MATERIAL AND METHODS The starch-clay composites were prepared by heating a dispersion of the starch in distilled water, then precipitating the dispersion with an equal volume of 95% ethanol.
33125195	1	60	used	used	277:280	arg2	excipients					307:316	directly compressible excipients	285:316	directly compressible excipients in tablet formulations	285:339	BACKGROUND Co-processing starch with clay nanocomposite has been shown to yield a new class of materials, potentially with better properties than pristine starch, that could be used as directly compressible excipients in tablet formulations.
33125195	1	60	used	used	277:280	arg2	starch					255:260	pristine starch	246:260	pristine starch	246:260	BACKGROUND Co-processing starch with clay nanocomposite has been shown to yield a new class of materials, potentially with better properties than pristine starch, that could be used as directly compressible excipients in tablet formulations.
33125195	1	61	with	starch	125:130	arg1	nanocomposite					142:154	clay nanocomposite	137:154	clay nanocomposite	137:154	BACKGROUND Co-processing starch with clay nanocomposite has been shown to yield a new class of materials, potentially with better properties than pristine starch, that could be used as directly compressible excipients in tablet formulations.
33125195	2	62	from	excipient	668:676	arg1	formulations					697:708	tramadol tablet formulations	681:708	tramadol tablet formulations	681:708	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	3	63	theme	different	726:734	arg1	ratios					751:756	different starch-to-clay ratios	726:756	different starch-to-clay ratios	726:756	The effects of different starch-to-clay ratios on the material and drug release properties of the resulting tablets were evaluated.
33125195	1	64	theme	materials	195:203	arg1	class					186:190	a new class	180:190	a new class of materials	180:203	BACKGROUND Co-processing starch with clay nanocomposite has been shown to yield a new class of materials, potentially with better properties than pristine starch, that could be used as directly compressible excipients in tablet formulations.
33125195	8	65	theme	pristine	1488:1495	arg1	starches					1497:1504	the pristine starches	1484:1504	the pristine starches	1484:1504	The co-processed starch-MMT biocomposites exhibited improved flowability and compressibility over the pristine starches.
33125195	10	66	theme	composites	1710:1719	arg1	related					1730:1736	related	1730:1736	related	1730:1736	The properties of the starch-clay composites were not related to the botanical source of the starches.
33125195	10	66	theme	composites	1710:1719	arg1	properties					1680:1689	The properties	1676:1689	The properties of the starch-clay composites	1676:1719	The properties of the starch-clay composites were not related to the botanical source of the starches.
33125195	7	67	with	composites	1301:1310	arg1	properties					1347:1356	different material and tablet properties	1317:1356	different material and tablet properties	1317:1356	RESULTS Co-processing MMT with the starches yielded starch-clay composites with different material and tablet properties than the pristine starches.
33125195	5	68	theme	starch-clay	1038:1048	arg1	excipients					1111:1120	direct compression excipients	1092:1120	direct compression excipients for the preparation of tramadol tablets	1092:1160	The starch-clay composites were characterized and used as direct compression excipients for the preparation of tramadol tablets.
33125195	5	68	theme	starch-clay	1038:1048	arg1	composites					1050:1059	The starch-clay composites	1034:1059	The starch-clay composites	1034:1059	The starch-clay composites were characterized and used as direct compression excipients for the preparation of tramadol tablets.
33125195	2	69	theme	Moench	501:506	arg1	grains					508:513	Sorghum bicolor L. Moench grains	482:513	Sorghum bicolor L. Moench grains	482:513	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	6	70	theme	tablets	1213:1219	arg1	mechanical					1167:1176	mechanical	1167:1176	mechanical	1167:1176	The mechanical and drug release properties of the tablets were evaluated.
33125195	1	71	theme	tablet	321:326	arg1	formulations					328:339	tablet formulations	321:339	tablet formulations	321:339	BACKGROUND Co-processing starch with clay nanocomposite has been shown to yield a new class of materials, potentially with better properties than pristine starch, that could be used as directly compressible excipients in tablet formulations.
33125195	9	72	theme	release	1531:1537	arg1	properties					1539:1548	The mechanical and drug release properties	1507:1548	properties	1539:1548	The mechanical and drug release properties of tramadol tablets containing starch-clay composites were significantly better than those containing only pristine starches.
33125195	4	73	theme	starch-clay	868:878	arg1	composites					880:889	The starch-clay composites	864:889	The starch-clay composites	864:889	MATERIAL AND METHODS The starch-clay composites were prepared by heating a dispersion of the starch in distilled water, then precipitating the dispersion with an equal volume of 95% ethanol.
33125195	2	74	theme	compressible	655:666	arg1	excipient					668:676	a directly compressible excipient	644:676	a directly compressible excipient in tramadol tablet formulations	644:708	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	2	74	theme	compressible	655:666	arg1	OBJECTIVES					342:351	OBJECTIVES	342:351	OBJECTIVES In this study	342:365	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	3	75	theme	ratios	751:756	arg1	effects					715:721	The effects	711:721	The effects of different starch-to-clay ratios on the material and drug release properties of the resulting tablets	711:825	The effects of different starch-to-clay ratios on the material and drug release properties of the resulting tablets were evaluated.
33125195	7	76	theme	pristine	1367:1374	arg1	starches					1376:1383	the pristine starches	1363:1383	the pristine starches	1363:1383	RESULTS Co-processing MMT with the starches yielded starch-clay composites with different material and tablet properties than the pristine starches.
33125195	5	77	used	used	1084:1087	arg2	excipients					1111:1120	direct compression excipients	1092:1120	direct compression excipients for the preparation of tramadol tablets	1092:1160	The starch-clay composites were characterized and used as direct compression excipients for the preparation of tramadol tablets.
33125195	5	77	used	used	1084:1087	arg2	composites					1050:1059	The starch-clay composites	1034:1059	The starch-clay composites	1034:1059	The starch-clay composites were characterized and used as direct compression excipients for the preparation of tramadol tablets.
33125195	0	78	theme	tramadol	70:77	arg1	formulations					86:97	tramadol tablet formulations	70:97	tramadol tablet formulations	70:97	Evaluation of starch-clay composites as a pharmaceutical excipient in tramadol tablet formulations.
33125195	9	79	theme	tablets	1562:1568	arg1	better					1623:1628	better	1623:1628	better	1623:1628	The mechanical and drug release properties of tramadol tablets containing starch-clay composites were significantly better than those containing only pristine starches.
33125195	9	79	theme	tablets	1562:1568	arg1	mechanical					1511:1520	mechanical	1511:1520	mechanical	1511:1520	The mechanical and drug release properties of tramadol tablets containing starch-clay composites were significantly better than those containing only pristine starches.
33125195	4	80	theme	distilled	946:954	arg1	water					956:960	distilled water	946:960	distilled water	946:960	MATERIAL AND METHODS The starch-clay composites were prepared by heating a dispersion of the starch in distilled water, then precipitating the dispersion with an equal volume of 95% ethanol.
33125195	9	81	theme	only	1652:1655	arg1	starches					1666:1673	only pristine starches	1652:1673	only pristine starches	1652:1673	The mechanical and drug release properties of tramadol tablets containing starch-clay composites were significantly better than those containing only pristine starches.
33125195	2	82	theme	tablet	690:695	arg1	formulations					697:708	tramadol tablet formulations	681:708	tramadol tablet formulations	681:708	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	5	83	theme	compression	1099:1109	arg1	composites					1050:1059	The starch-clay composites	1034:1059	The starch-clay composites	1034:1059	The starch-clay composites were characterized and used as direct compression excipients for the preparation of tramadol tablets.
33125195	5	83	theme	compression	1099:1109	arg1	excipients					1111:1120	direct compression excipients	1092:1120	direct compression excipients for the preparation of tramadol tablets	1092:1160	The starch-clay composites were characterized and used as direct compression excipients for the preparation of tramadol tablets.
33125195	9	84	theme	starch-clay	1581:1591	arg1	composites					1593:1602	starch-clay composites	1581:1602	starch-clay composites	1581:1602	The mechanical and drug release properties of tramadol tablets containing starch-clay composites were significantly better than those containing only pristine starches.
33125195	3	85	theme	resulting	809:817	arg1	tablets					819:825	the resulting tablets	805:825	the resulting tablets	805:825	The effects of different starch-to-clay ratios on the material and drug release properties of the resulting tablets were evaluated.
33125195	2	86	theme	bicolor	490:496	arg1	grains					508:513	Sorghum bicolor L. Moench grains	482:513	Sorghum bicolor L. Moench grains	482:513	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	0	87	from	excipient	57:65	arg1	formulations					86:97	tramadol tablet formulations	70:97	tramadol tablet formulations	70:97	Evaluation of starch-clay composites as a pharmaceutical excipient in tramadol tablet formulations.
33125195	4	88	theme	starch	936:941	arg1	dispersion					918:927	a dispersion	916:927	a dispersion of the starch	916:941	MATERIAL AND METHODS The starch-clay composites were prepared by heating a dispersion of the starch in distilled water, then precipitating the dispersion with an equal volume of 95% ethanol.
33125195	3	89	from	effects	715:721	arg1	material					765:772	material	765:772	material	765:772	The effects of different starch-to-clay ratios on the material and drug release properties of the resulting tablets were evaluated.
33125195	3	89	from	effects	715:721	arg1	properties					791:800	the material and drug release properties	761:800	properties	791:800	The effects of different starch-to-clay ratios on the material and drug release properties of the resulting tablets were evaluated.
33125195	2	90	theme	botanical	384:392	arg1	sources					394:400	3 botanical sources	382:400	3 botanical sources	382:400	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	7	91	theme	different	1317:1325	arg1	properties					1347:1356	different material and tablet properties	1317:1356	different material and tablet properties	1317:1356	RESULTS Co-processing MMT with the starches yielded starch-clay composites with different material and tablet properties than the pristine starches.
33125195	0	92	theme	composites	26:35	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of starch-clay composites as a pharmaceutical excipient in tramadol tablet formulations.	0:98	Evaluation of starch-clay composites as a pharmaceutical excipient in tramadol tablet formulations.
33125195	1	93	theme	Co-processing	111:123	arg1	starch					125:130	BACKGROUND Co-processing starch	100:130	BACKGROUND Co-processing starch with clay nanocomposite	100:154	BACKGROUND Co-processing starch with clay nanocomposite has been shown to yield a new class of materials, potentially with better properties than pristine starch, that could be used as directly compressible excipients in tablet formulations.
33125195	8	94	theme	starch-MMT	1403:1412	arg1	biocomposites					1414:1426	The co-processed starch-MMT biocomposites	1386:1426	The co-processed starch-MMT biocomposites	1386:1426	The co-processed starch-MMT biocomposites exhibited improved flowability and compressibility over the pristine starches.
33125195	5	95	theme	tramadol	1145:1152	arg1	tablets					1154:1160	tramadol tablets	1145:1160	tramadol tablets	1145:1160	The starch-clay composites were characterized and used as direct compression excipients for the preparation of tramadol tablets.
33125195	2	96	from	grains	508:513	arg1	starch					416:421	millet starch	409:421	millet starch from Pennistum glaucum (L) RBr grains	409:459	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	2	96	from	grains	508:513	arg1	starch					470:475	sorghum starch	462:475	sorghum starch from Sorghum bicolor L. Moench grains	462:513	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	2	96	from	grains	508:513	arg1	starch					527:532	cocoyam starch	519:532	cocoyam starch from Colocasia esculenta L. Schott tubers	519:574	OBJECTIVES In this study, starches from 3 botanical sources, i.e., millet starch from Pennistum glaucum (L) RBr grains, sorghum starch from Sorghum bicolor L. Moench grains and cocoyam starch from Colocasia esculenta L. Schott tubers, were co-processed with montmorillonite clay (MMT) and evaluated as a directly compressible excipient in tramadol tablet formulations.
33125195	11	97	dep	CONCLUSIONS	1779:1789	arg1	showed					1801:1806	showed	1801:1806	showed that starch-clay biocomposites could be used in the controlled release of tramadol	1801:1889	CONCLUSIONS The study showed that starch-clay biocomposites could be used in the controlled release of tramadol.
33125195	4	98	theme	equal	1005:1009	arg1	volume					1011:1016	an equal volume	1002:1016	an equal volume of 95% ethanol	1002:1031	MATERIAL AND METHODS The starch-clay composites were prepared by heating a dispersion of the starch in distilled water, then precipitating the dispersion with an equal volume of 95% ethanol.
33125195	1	99	theme	pristine	246:253	arg1	excipients					307:316	directly compressible excipients	285:316	directly compressible excipients in tablet formulations	285:339	BACKGROUND Co-processing starch with clay nanocomposite has been shown to yield a new class of materials, potentially with better properties than pristine starch, that could be used as directly compressible excipients in tablet formulations.
33125195	1	99	theme	pristine	246:253	arg1	starch					255:260	pristine starch	246:260	pristine starch	246:260	BACKGROUND Co-processing starch with clay nanocomposite has been shown to yield a new class of materials, potentially with better properties than pristine starch, that could be used as directly compressible excipients in tablet formulations.
32757263	0	0	theme	Deposited	90:98	arg1	Matrix					128:133	Deposited Cartilaginous Extracellular Matrix	90:133	Deposited Cartilaginous Extracellular Matrix	90:133	Hyaluronic Acid-Based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix.
32757263	8	1	theme	dual	1515:1518	arg1	function					1520:1527	dual function	1515:1527	dual function of a supplement enabling PCL-supported bioprinting and at the same time improving biological properties of the resulting constructs	1515:1659	The study contributes to effective bioink development, demonstrating dual function of a supplement enabling PCL-supported bioprinting and at the same time improving biological properties of the resulting constructs.
32757263	8	2	theme	PCL-supported	1554:1566	arg1	bioprinting					1568:1578	PCL-supported bioprinting	1554:1578	PCL-supported bioprinting	1554:1578	The study contributes to effective bioink development, demonstrating dual function of a supplement enabling PCL-supported bioprinting and at the same time improving biological properties of the resulting constructs.
32757263	1	3	theme	high	168:171	arg1	concentrations					173:186	high concentrations	168:186	high concentrations of polymeric materials	168:209	In 3D bioprinting, bioinks with high concentrations of polymeric materials are frequently used to enable fabrication of 3D cell-hydrogel constructs with sufficient stability.
32757263	1	4	theme	constructs	273:282	arg1	fabrication					241:251	fabrication	241:251	fabrication of 3D cell-hydrogel constructs with sufficient stability	241:308	In 3D bioprinting, bioinks with high concentrations of polymeric materials are frequently used to enable fabrication of 3D cell-hydrogel constructs with sufficient stability.
32757263	5	5	theme	-supported	780:789	arg1	bioprinting					794:804	poly(ε-caprolactone) (PCL)-supported 3D bioprinting	754:804	poly(ε-caprolactone) (PCL)-supported 3D bioprinting	754:804	To adapt bioinks to poly(ε-caprolactone) (PCL)-supported 3D bioprinting, the gels are further supplemented with 1 wt% unmodified high molecular weight HA (hmHA) and chondrogenic differentiation of incorporated human mesenchymal stromal cells is assessed.
32757263	0	6	theme	Extracellular	114:126	arg1	Matrix					128:133	Deposited Cartilaginous Extracellular Matrix	90:133	Deposited Cartilaginous Extracellular Matrix	90:133	Hyaluronic Acid-Based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix.
32757263	1	7	with	bioinks	155:161	arg1	concentrations					173:186	high concentrations	168:186	high concentrations of polymeric materials	168:209	In 3D bioprinting, bioinks with high concentrations of polymeric materials are frequently used to enable fabrication of 3D cell-hydrogel constructs with sufficient stability.
32757263	8	8	theme	constructs	1650:1659	arg1	properties					1622:1631	biological properties	1611:1631	biological properties of the resulting constructs	1611:1659	The study contributes to effective bioink development, demonstrating dual function of a supplement enabling PCL-supported bioprinting and at the same time improving biological properties of the resulting constructs.
32757263	6	9	theme	independent	1174:1184	arg1	constructs					1162:1171	the constructs	1158:1171	the constructs	1158:1171	Strikingly, addition of hmHA to gels with a low polymer content (3 wt%) results in distinct increase of construct quality with a homogeneous ECM distribution throughout the constructs, independent of the printing process.
32757263	6	10	theme	homogeneous	1118:1128	arg1	distribution					1134:1145	a homogeneous ECM distribution	1116:1145	a homogeneous ECM distribution throughout the constructs, independent of the printing process	1116:1208	Strikingly, addition of hmHA to gels with a low polymer content (3 wt%) results in distinct increase of construct quality with a homogeneous ECM distribution throughout the constructs, independent of the printing process.
32757263	1	11	theme	sufficient	289:298	arg1	stability					300:308	sufficient stability	289:308	sufficient stability	289:308	In 3D bioprinting, bioinks with high concentrations of polymeric materials are frequently used to enable fabrication of 3D cell-hydrogel constructs with sufficient stability.
32757263	0	12	theme	Cartilaginous	100:112	arg1	Matrix					128:133	Deposited Cartilaginous Extracellular Matrix	90:133	Deposited Cartilaginous Extracellular Matrix	90:133	Hyaluronic Acid-Based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix.
32757263	3	13	theme	cartilage	571:579	arg1	regeneration					581:592	cartilage regeneration	571:592	cartilage regeneration	571:592	Therefore, this study investigates bioink compositions based on hyaluronic acid (HA), an attractive material for cartilage regeneration, which allow for reduction of polymer content.
32757263	2	14	theme	inhomogeneous	385:397	arg1	distribution					399:410	an inhomogeneous distribution	382:410	an inhomogeneous distribution of newly produced extracellular matrix (ECM)	382:455	However, this is often associated with restricted cell bioactivity and an inhomogeneous distribution of newly produced extracellular matrix (ECM).
32757263	8	15	theme	biological	1611:1620	arg1	properties					1622:1631	biological properties	1611:1631	biological properties of the resulting constructs	1611:1659	The study contributes to effective bioink development, demonstrating dual function of a supplement enabling PCL-supported bioprinting and at the same time improving biological properties of the resulting constructs.
32757263	3	16	theme	hyaluronic	522:531	arg1	acid					533:536	hyaluronic acid	522:536	hyaluronic acid (HA)	522:541	Therefore, this study investigates bioink compositions based on hyaluronic acid (HA), an attractive material for cartilage regeneration, which allow for reduction of polymer content.
32757263	3	16	theme	hyaluronic	522:531	arg1	material					558:565	an attractive material	544:565	an attractive material for cartilage regeneration	544:592	Therefore, this study investigates bioink compositions based on hyaluronic acid (HA), an attractive material for cartilage regeneration, which allow for reduction of polymer content.
32757263	3	16	theme	hyaluronic	522:531	arg1	HA					539:540	HA	539:540	HA	539:540	Therefore, this study investigates bioink compositions based on hyaluronic acid (HA), an attractive material for cartilage regeneration, which allow for reduction of polymer content.
32757263	7	17	theme	increased	1276:1284	arg1	stiffness					1296:1304	increased construct stiffness	1276:1304	increased construct stiffness after chondrogenic differentiation	1276:1339	Improved ECM distribution in those constructs is associated with increased construct stiffness after chondrogenic differentiation, as compared to higher concentrated constructs (10 wt%), which only show pericellular matrix deposition.
32757263	8	18	dep	supplement	1534:1543	arg1	enabling					1545:1552	enabling	1545:1552	enabling PCL-supported bioprinting	1545:1578	The study contributes to effective bioink development, demonstrating dual function of a supplement enabling PCL-supported bioprinting and at the same time improving biological properties of the resulting constructs.
32757263	8	18	dep	supplement	1534:1543	arg1	time					1596:1599	the same time	1587:1599	the same time	1587:1599	The study contributes to effective bioink development, demonstrating dual function of a supplement enabling PCL-supported bioprinting and at the same time improving biological properties of the resulting constructs.
32757263	6	19	theme	distinct	1072:1079	arg1	increase					1081:1088	distinct increase	1072:1088	distinct increase of construct quality with a homogeneous ECM distribution throughout the constructs, independent of the printing process	1072:1208	Strikingly, addition of hmHA to gels with a low polymer content (3 wt%) results in distinct increase of construct quality with a homogeneous ECM distribution throughout the constructs, independent of the printing process.
32757263	3	20	theme	attractive	547:556	arg1	acid					533:536	hyaluronic acid	522:536	hyaluronic acid (HA)	522:541	Therefore, this study investigates bioink compositions based on hyaluronic acid (HA), an attractive material for cartilage regeneration, which allow for reduction of polymer content.
32757263	3	20	theme	attractive	547:556	arg1	material					558:565	an attractive material	544:565	an attractive material for cartilage regeneration	544:592	Therefore, this study investigates bioink compositions based on hyaluronic acid (HA), an attractive material for cartilage regeneration, which allow for reduction of polymer content.
32757263	2	21	theme	produced	421:428	arg1	ECM					452:454	ECM	452:454	ECM	452:454	However, this is often associated with restricted cell bioactivity and an inhomogeneous distribution of newly produced extracellular matrix (ECM).
32757263	2	21	theme	produced	421:428	arg1	matrix					444:449	extracellular matrix	430:449	newly produced extracellular matrix (ECM)	415:455	However, this is often associated with restricted cell bioactivity and an inhomogeneous distribution of newly produced extracellular matrix (ECM).
32757263	2	22	theme	matrix	444:449	arg1	bioactivity					366:376	restricted cell bioactivity	350:376	restricted cell bioactivity	350:376	However, this is often associated with restricted cell bioactivity and an inhomogeneous distribution of newly produced extracellular matrix (ECM).
32757263	2	22	theme	matrix	444:449	arg1	distribution					399:410	an inhomogeneous distribution	382:410	an inhomogeneous distribution of newly produced extracellular matrix (ECM)	382:455	However, this is often associated with restricted cell bioactivity and an inhomogeneous distribution of newly produced extracellular matrix (ECM).
32757263	0	23	theme	Matrix	128:133	arg1	Quality					79:85	Quality	79:85	Quality of Deposited Cartilaginous Extracellular Matrix	79:133	Hyaluronic Acid-Based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix.
32757263	5	24	theme	3D	791:792	arg1	bioprinting					794:804	poly(ε-caprolactone) (PCL)-supported 3D bioprinting	754:804	poly(ε-caprolactone) (PCL)-supported 3D bioprinting	754:804	To adapt bioinks to poly(ε-caprolactone) (PCL)-supported 3D bioprinting, the gels are further supplemented with 1 wt% unmodified high molecular weight HA (hmHA) and chondrogenic differentiation of incorporated human mesenchymal stromal cells is assessed.
32757263	1	25	theme	polymeric	191:199	arg1	materials					201:209	polymeric materials	191:209	polymeric materials	191:209	In 3D bioprinting, bioinks with high concentrations of polymeric materials are frequently used to enable fabrication of 3D cell-hydrogel constructs with sufficient stability.
32757263	0	26	theme	Acid-Based	11:20	arg1	Composition					29:39	Hyaluronic Acid-Based Bioink Composition	0:39	Hyaluronic Acid-Based Bioink Composition	0:39	Hyaluronic Acid-Based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix.
32757263	6	27	with	increase	1081:1088	arg1	distribution					1134:1145	a homogeneous ECM distribution	1116:1145	a homogeneous ECM distribution throughout the constructs, independent of the printing process	1116:1208	Strikingly, addition of hmHA to gels with a low polymer content (3 wt%) results in distinct increase of construct quality with a homogeneous ECM distribution throughout the constructs, independent of the printing process.
32757263	7	28	theme	Improved	1211:1218	arg1	distribution					1224:1235	Improved ECM distribution	1211:1235	Improved ECM distribution in those constructs	1211:1255	Improved ECM distribution in those constructs is associated with increased construct stiffness after chondrogenic differentiation, as compared to higher concentrated constructs (10 wt%), which only show pericellular matrix deposition.
32757263	7	29	theme	ECM	1220:1222	arg1	distribution					1224:1235	Improved ECM distribution	1211:1235	Improved ECM distribution in those constructs	1211:1255	Improved ECM distribution in those constructs is associated with increased construct stiffness after chondrogenic differentiation, as compared to higher concentrated constructs (10 wt%), which only show pericellular matrix deposition.
32757263	5	30	theme	human	944:948	arg1	cells					970:974	incorporated human mesenchymal stromal cells	931:974	incorporated human mesenchymal stromal cells	931:974	To adapt bioinks to poly(ε-caprolactone) (PCL)-supported 3D bioprinting, the gels are further supplemented with 1 wt% unmodified high molecular weight HA (hmHA) and chondrogenic differentiation of incorporated human mesenchymal stromal cells is assessed.
32757263	8	31	theme	supplement	1534:1543	arg1	function					1520:1527	dual function	1515:1527	dual function of a supplement enabling PCL-supported bioprinting and at the same time improving biological properties of the resulting constructs	1515:1659	The study contributes to effective bioink development, demonstrating dual function of a supplement enabling PCL-supported bioprinting and at the same time improving biological properties of the resulting constructs.
32757263	1	32	theme	materials	201:209	arg1	concentrations					173:186	high concentrations	168:186	high concentrations of polymeric materials	168:209	In 3D bioprinting, bioinks with high concentrations of polymeric materials are frequently used to enable fabrication of 3D cell-hydrogel constructs with sufficient stability.
32757263	0	33	theme	Hyaluronic	0:9	arg1	Composition					29:39	Hyaluronic Acid-Based Bioink Composition	0:39	Hyaluronic Acid-Based Bioink Composition	0:39	Hyaluronic Acid-Based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix.
32757263	7	34	from	distribution	1224:1235	arg1	constructs					1246:1255	those constructs	1240:1255	those constructs	1240:1255	Improved ECM distribution in those constructs is associated with increased construct stiffness after chondrogenic differentiation, as compared to higher concentrated constructs (10 wt%), which only show pericellular matrix deposition.
32757263	5	35	theme	molecular	868:876	arg1	weight					878:883	1 wt% unmodified high molecular weight HA	846:886	1 wt% unmodified high molecular weight HA (hmHA)	846:893	To adapt bioinks to poly(ε-caprolactone) (PCL)-supported 3D bioprinting, the gels are further supplemented with 1 wt% unmodified high molecular weight HA (hmHA) and chondrogenic differentiation of incorporated human mesenchymal stromal cells is assessed.
32757263	5	35	theme	molecular	868:876	arg1	hmHA					889:892	hmHA	889:892	hmHA	889:892	To adapt bioinks to poly(ε-caprolactone) (PCL)-supported 3D bioprinting, the gels are further supplemented with 1 wt% unmodified high molecular weight HA (hmHA) and chondrogenic differentiation of incorporated human mesenchymal stromal cells is assessed.
32757263	6	36	theme	hmHA	1013:1016	arg1	addition					1001:1008	addition	1001:1008	addition of hmHA to gels with a low polymer content (3 wt%)	1001:1059	Strikingly, addition of hmHA to gels with a low polymer content (3 wt%) results in distinct increase of construct quality with a homogeneous ECM distribution throughout the constructs, independent of the printing process.
32757263	8	37	theme	resulting	1640:1648	arg1	constructs					1650:1659	the resulting constructs	1636:1659	the resulting constructs	1636:1659	The study contributes to effective bioink development, demonstrating dual function of a supplement enabling PCL-supported bioprinting and at the same time improving biological properties of the resulting constructs.
32757263	7	38	theme	pericellular	1414:1425	arg1	deposition					1434:1443	pericellular matrix deposition	1414:1443	pericellular matrix deposition	1414:1443	Improved ECM distribution in those constructs is associated with increased construct stiffness after chondrogenic differentiation, as compared to higher concentrated constructs (10 wt%), which only show pericellular matrix deposition.
32757263	5	39	theme	incorporated	931:942	arg1	cells					970:974	incorporated human mesenchymal stromal cells	931:974	incorporated human mesenchymal stromal cells	931:974	To adapt bioinks to poly(ε-caprolactone) (PCL)-supported 3D bioprinting, the gels are further supplemented with 1 wt% unmodified high molecular weight HA (hmHA) and chondrogenic differentiation of incorporated human mesenchymal stromal cells is assessed.
32757263	5	40	theme	stromal	962:968	arg1	cells					970:974	incorporated human mesenchymal stromal cells	931:974	incorporated human mesenchymal stromal cells	931:974	To adapt bioinks to poly(ε-caprolactone) (PCL)-supported 3D bioprinting, the gels are further supplemented with 1 wt% unmodified high molecular weight HA (hmHA) and chondrogenic differentiation of incorporated human mesenchymal stromal cells is assessed.
32757263	4	41	from	poly	673:676	arg1	concentrations					699:712	varying concentrations	691:712	varying concentrations	691:712	Thiolated HA and allyl-modified poly(glycidol) in varying concentrations are UV-crosslinked.
32757263	4	42	theme	allyl-modified	658:671	arg1	poly					673:676	allyl-modified poly	658:676	allyl-modified poly(glycidol)	658:686	Thiolated HA and allyl-modified poly(glycidol) in varying concentrations are UV-crosslinked.
32757263	4	42	theme	allyl-modified	658:671	arg1	glycidol					678:685	glycidol	678:685	glycidol	678:685	Thiolated HA and allyl-modified poly(glycidol) in varying concentrations are UV-crosslinked.
32757263	0	43	theme	Bioink	22:27	arg1	Composition					29:39	Hyaluronic Acid-Based Bioink Composition	0:39	Hyaluronic Acid-Based Bioink Composition	0:39	Hyaluronic Acid-Based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix.
32757263	8	44	theme	same	1591:1594	arg1	time					1596:1599	the same time	1587:1599	the same time	1587:1599	The study contributes to effective bioink development, demonstrating dual function of a supplement enabling PCL-supported bioprinting and at the same time improving biological properties of the resulting constructs.
32757263	5	45	theme	mesenchymal	950:960	arg1	cells					970:974	incorporated human mesenchymal stromal cells	931:974	incorporated human mesenchymal stromal cells	931:974	To adapt bioinks to poly(ε-caprolactone) (PCL)-supported 3D bioprinting, the gels are further supplemented with 1 wt% unmodified high molecular weight HA (hmHA) and chondrogenic differentiation of incorporated human mesenchymal stromal cells is assessed.
32757263	5	46	theme	cells	970:974	arg1	differentiation					912:926	chondrogenic differentiation	899:926	chondrogenic differentiation of incorporated human mesenchymal stromal cells	899:974	To adapt bioinks to poly(ε-caprolactone) (PCL)-supported 3D bioprinting, the gels are further supplemented with 1 wt% unmodified high molecular weight HA (hmHA) and chondrogenic differentiation of incorporated human mesenchymal stromal cells is assessed.
32757263	5	47	theme	1 wt	846:849	arg1	weight					878:883	1 wt% unmodified high molecular weight HA	846:886	1 wt% unmodified high molecular weight HA (hmHA)	846:893	To adapt bioinks to poly(ε-caprolactone) (PCL)-supported 3D bioprinting, the gels are further supplemented with 1 wt% unmodified high molecular weight HA (hmHA) and chondrogenic differentiation of incorporated human mesenchymal stromal cells is assessed.
32757263	5	47	theme	1 wt	846:849	arg1	hmHA					889:892	hmHA	889:892	hmHA	889:892	To adapt bioinks to poly(ε-caprolactone) (PCL)-supported 3D bioprinting, the gels are further supplemented with 1 wt% unmodified high molecular weight HA (hmHA) and chondrogenic differentiation of incorporated human mesenchymal stromal cells is assessed.
32757263	0	48	theme	3D	50:51	arg1	Bioprinting					53:63	3D Bioprinting	50:63	3D Bioprinting	50:63	Hyaluronic Acid-Based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix.
32757263	4	49	theme	varying	691:697	arg1	concentrations					699:712	varying concentrations	691:712	varying concentrations	691:712	Thiolated HA and allyl-modified poly(glycidol) in varying concentrations are UV-crosslinked.
32757263	6	50	theme	polymer	1037:1043	arg1	content					1045:1051	a low polymer content	1031:1051	a low polymer content (3 wt%)	1031:1059	Strikingly, addition of hmHA to gels with a low polymer content (3 wt%) results in distinct increase of construct quality with a homogeneous ECM distribution throughout the constructs, independent of the printing process.
32757263	6	50	theme	polymer	1037:1043	arg1	%					1058:1058	3 wt%	1054:1058	3 wt%	1054:1058	Strikingly, addition of hmHA to gels with a low polymer content (3 wt%) results in distinct increase of construct quality with a homogeneous ECM distribution throughout the constructs, independent of the printing process.
32757263	6	51	with	gels	1021:1024	arg1	content					1045:1051	a low polymer content	1031:1051	a low polymer content (3 wt%)	1031:1059	Strikingly, addition of hmHA to gels with a low polymer content (3 wt%) results in distinct increase of construct quality with a homogeneous ECM distribution throughout the constructs, independent of the printing process.
32757263	6	51	with	gels	1021:1024	arg1	%					1058:1058	3 wt%	1054:1058	3 wt%	1054:1058	Strikingly, addition of hmHA to gels with a low polymer content (3 wt%) results in distinct increase of construct quality with a homogeneous ECM distribution throughout the constructs, independent of the printing process.
32757263	5	52	theme	%	850:850	arg1	weight					878:883	1 wt% unmodified high molecular weight HA	846:886	1 wt% unmodified high molecular weight HA (hmHA)	846:893	To adapt bioinks to poly(ε-caprolactone) (PCL)-supported 3D bioprinting, the gels are further supplemented with 1 wt% unmodified high molecular weight HA (hmHA) and chondrogenic differentiation of incorporated human mesenchymal stromal cells is assessed.
32757263	5	52	theme	%	850:850	arg1	hmHA					889:892	hmHA	889:892	hmHA	889:892	To adapt bioinks to poly(ε-caprolactone) (PCL)-supported 3D bioprinting, the gels are further supplemented with 1 wt% unmodified high molecular weight HA (hmHA) and chondrogenic differentiation of incorporated human mesenchymal stromal cells is assessed.
32757263	5	53	theme	high	863:866	arg1	weight					878:883	1 wt% unmodified high molecular weight HA	846:886	1 wt% unmodified high molecular weight HA (hmHA)	846:893	To adapt bioinks to poly(ε-caprolactone) (PCL)-supported 3D bioprinting, the gels are further supplemented with 1 wt% unmodified high molecular weight HA (hmHA) and chondrogenic differentiation of incorporated human mesenchymal stromal cells is assessed.
32757263	5	53	theme	high	863:866	arg1	hmHA					889:892	hmHA	889:892	hmHA	889:892	To adapt bioinks to poly(ε-caprolactone) (PCL)-supported 3D bioprinting, the gels are further supplemented with 1 wt% unmodified high molecular weight HA (hmHA) and chondrogenic differentiation of incorporated human mesenchymal stromal cells is assessed.
32757263	8	54	theme	effective	1471:1479	arg1	development					1488:1498	effective bioink development	1471:1498	effective bioink development	1471:1498	The study contributes to effective bioink development, demonstrating dual function of a supplement enabling PCL-supported bioprinting and at the same time improving biological properties of the resulting constructs.
32757263	3	55	theme	polymer	624:630	arg1	content					632:638	polymer content	624:638	polymer content	624:638	Therefore, this study investigates bioink compositions based on hyaluronic acid (HA), an attractive material for cartilage regeneration, which allow for reduction of polymer content.
32757263	3	56	theme	bioink	493:498	arg1	compositions					500:511	bioink compositions	493:511	bioink compositions based on hyaluronic acid (HA), an attractive material for cartilage regeneration, which allow for reduction of polymer content	493:638	Therefore, this study investigates bioink compositions based on hyaluronic acid (HA), an attractive material for cartilage regeneration, which allow for reduction of polymer content.
32757263	5	57	theme	poly	754:757	arg1	bioprinting					794:804	poly(ε-caprolactone) (PCL)-supported 3D bioprinting	754:804	poly(ε-caprolactone) (PCL)-supported 3D bioprinting	754:804	To adapt bioinks to poly(ε-caprolactone) (PCL)-supported 3D bioprinting, the gels are further supplemented with 1 wt% unmodified high molecular weight HA (hmHA) and chondrogenic differentiation of incorporated human mesenchymal stromal cells is assessed.
32757263	6	58	theme	low	1033:1035	arg1	content					1045:1051	a low polymer content	1031:1051	a low polymer content (3 wt%)	1031:1059	Strikingly, addition of hmHA to gels with a low polymer content (3 wt%) results in distinct increase of construct quality with a homogeneous ECM distribution throughout the constructs, independent of the printing process.
32757263	6	58	theme	low	1033:1035	arg1	%					1058:1058	3 wt%	1054:1058	3 wt%	1054:1058	Strikingly, addition of hmHA to gels with a low polymer content (3 wt%) results in distinct increase of construct quality with a homogeneous ECM distribution throughout the constructs, independent of the printing process.
32757263	1	59	used	used	226:229	arg2	bioinks					155:161	bioinks	155:161	bioinks with high concentrations of polymeric materials	155:209	In 3D bioprinting, bioinks with high concentrations of polymeric materials are frequently used to enable fabrication of 3D cell-hydrogel constructs with sufficient stability.
32757263	5	60	theme	chondrogenic	899:910	arg1	differentiation					912:926	chondrogenic differentiation	899:926	chondrogenic differentiation of incorporated human mesenchymal stromal cells	899:974	To adapt bioinks to poly(ε-caprolactone) (PCL)-supported 3D bioprinting, the gels are further supplemented with 1 wt% unmodified high molecular weight HA (hmHA) and chondrogenic differentiation of incorporated human mesenchymal stromal cells is assessed.
32757263	1	61	with	constructs	273:282	arg1	stability					300:308	sufficient stability	289:308	sufficient stability	289:308	In 3D bioprinting, bioinks with high concentrations of polymeric materials are frequently used to enable fabrication of 3D cell-hydrogel constructs with sufficient stability.
32757263	1	62	theme	3D	139:140	arg1	bioprinting					142:152	3D bioprinting	139:152	3D bioprinting	139:152	In 3D bioprinting, bioinks with high concentrations of polymeric materials are frequently used to enable fabrication of 3D cell-hydrogel constructs with sufficient stability.
32757263	7	63	theme	concentrated	1364:1375	arg1	constructs					1377:1386	higher concentrated constructs	1357:1386	higher concentrated constructs (10 wt%)	1357:1395	Improved ECM distribution in those constructs is associated with increased construct stiffness after chondrogenic differentiation, as compared to higher concentrated constructs (10 wt%), which only show pericellular matrix deposition.
32757263	7	63	theme	concentrated	1364:1375	arg1	%					1394:1394	10 wt%	1389:1394	10 wt%	1389:1394	Improved ECM distribution in those constructs is associated with increased construct stiffness after chondrogenic differentiation, as compared to higher concentrated constructs (10 wt%), which only show pericellular matrix deposition.
32757263	8	64	dep	enabling	1545:1552	arg1	improving					1601:1609	improving	1601:1609	improving biological properties of the resulting constructs	1601:1659	The study contributes to effective bioink development, demonstrating dual function of a supplement enabling PCL-supported bioprinting and at the same time improving biological properties of the resulting constructs.
32757263	8	65	theme	bioink	1481:1486	arg1	development					1488:1498	effective bioink development	1471:1498	effective bioink development	1471:1498	The study contributes to effective bioink development, demonstrating dual function of a supplement enabling PCL-supported bioprinting and at the same time improving biological properties of the resulting constructs.
32757263	6	66	theme	process	1202:1208	arg1	independent					1174:1184	independent	1174:1184	independent	1174:1184	Strikingly, addition of hmHA to gels with a low polymer content (3 wt%) results in distinct increase of construct quality with a homogeneous ECM distribution throughout the constructs, independent of the printing process.
32757263	7	67	theme	matrix	1427:1432	arg1	deposition					1434:1443	pericellular matrix deposition	1414:1443	pericellular matrix deposition	1414:1443	Improved ECM distribution in those constructs is associated with increased construct stiffness after chondrogenic differentiation, as compared to higher concentrated constructs (10 wt%), which only show pericellular matrix deposition.
32757263	2	68	theme	cell	361:364	arg1	bioactivity					366:376	restricted cell bioactivity	350:376	restricted cell bioactivity	350:376	However, this is often associated with restricted cell bioactivity and an inhomogeneous distribution of newly produced extracellular matrix (ECM).
32757263	6	69	theme	ECM	1130:1132	arg1	distribution					1134:1145	a homogeneous ECM distribution	1116:1145	a homogeneous ECM distribution throughout the constructs, independent of the printing process	1116:1208	Strikingly, addition of hmHA to gels with a low polymer content (3 wt%) results in distinct increase of construct quality with a homogeneous ECM distribution throughout the constructs, independent of the printing process.
32757263	6	70	theme	construct	1093:1101	arg1	quality					1103:1109	construct quality	1093:1109	construct quality	1093:1109	Strikingly, addition of hmHA to gels with a low polymer content (3 wt%) results in distinct increase of construct quality with a homogeneous ECM distribution throughout the constructs, independent of the printing process.
32757263	7	71	theme	construct	1286:1294	arg1	stiffness					1296:1304	increased construct stiffness	1276:1304	increased construct stiffness after chondrogenic differentiation	1276:1339	Improved ECM distribution in those constructs is associated with increased construct stiffness after chondrogenic differentiation, as compared to higher concentrated constructs (10 wt%), which only show pericellular matrix deposition.
32757263	6	72	theme	printing	1193:1200	arg1	process					1202:1208	the printing process	1189:1208	the printing process	1189:1208	Strikingly, addition of hmHA to gels with a low polymer content (3 wt%) results in distinct increase of construct quality with a homogeneous ECM distribution throughout the constructs, independent of the printing process.
32757263	2	73	theme	restricted	350:359	arg1	bioactivity					366:376	restricted cell bioactivity	350:376	restricted cell bioactivity	350:376	However, this is often associated with restricted cell bioactivity and an inhomogeneous distribution of newly produced extracellular matrix (ECM).
32757263	6	74	theme	quality	1103:1109	arg1	increase					1081:1088	distinct increase	1072:1088	distinct increase of construct quality with a homogeneous ECM distribution throughout the constructs, independent of the printing process	1072:1208	Strikingly, addition of hmHA to gels with a low polymer content (3 wt%) results in distinct increase of construct quality with a homogeneous ECM distribution throughout the constructs, independent of the printing process.
32757263	4	75	from	HA	651:652	arg1	concentrations					699:712	varying concentrations	691:712	varying concentrations	691:712	Thiolated HA and allyl-modified poly(glycidol) in varying concentrations are UV-crosslinked.
32757263	5	76	theme	unmodified	852:861	arg1	weight					878:883	1 wt% unmodified high molecular weight HA	846:886	1 wt% unmodified high molecular weight HA (hmHA)	846:893	To adapt bioinks to poly(ε-caprolactone) (PCL)-supported 3D bioprinting, the gels are further supplemented with 1 wt% unmodified high molecular weight HA (hmHA) and chondrogenic differentiation of incorporated human mesenchymal stromal cells is assessed.
32757263	5	76	theme	unmodified	852:861	arg1	hmHA					889:892	hmHA	889:892	hmHA	889:892	To adapt bioinks to poly(ε-caprolactone) (PCL)-supported 3D bioprinting, the gels are further supplemented with 1 wt% unmodified high molecular weight HA (hmHA) and chondrogenic differentiation of incorporated human mesenchymal stromal cells is assessed.
32757263	3	77	theme	content	632:638	arg1	reduction					611:619	reduction	611:619	reduction of polymer content	611:638	Therefore, this study investigates bioink compositions based on hyaluronic acid (HA), an attractive material for cartilage regeneration, which allow for reduction of polymer content.
32757263	7	78	theme	chondrogenic	1312:1323	arg1	differentiation					1325:1339	chondrogenic differentiation	1312:1339	chondrogenic differentiation	1312:1339	Improved ECM distribution in those constructs is associated with increased construct stiffness after chondrogenic differentiation, as compared to higher concentrated constructs (10 wt%), which only show pericellular matrix deposition.
32757263	2	79	theme	extracellular	430:442	arg1	ECM					452:454	ECM	452:454	ECM	452:454	However, this is often associated with restricted cell bioactivity and an inhomogeneous distribution of newly produced extracellular matrix (ECM).
32757263	2	79	theme	extracellular	430:442	arg1	matrix					444:449	extracellular matrix	430:449	newly produced extracellular matrix (ECM)	415:455	However, this is often associated with restricted cell bioactivity and an inhomogeneous distribution of newly produced extracellular matrix (ECM).
32757263	1	80	theme	3D	256:257	arg1	constructs					273:282	3D cell-hydrogel constructs	256:282	3D cell-hydrogel constructs with sufficient stability	256:308	In 3D bioprinting, bioinks with high concentrations of polymeric materials are frequently used to enable fabrication of 3D cell-hydrogel constructs with sufficient stability.
32757263	4	81	theme	Thiolated	641:649	arg1	HA					651:652	Thiolated HA	641:652	Thiolated HA	641:652	Thiolated HA and allyl-modified poly(glycidol) in varying concentrations are UV-crosslinked.
32757263	7	82	theme	higher	1357:1362	arg1	constructs					1377:1386	higher concentrated constructs	1357:1386	higher concentrated constructs (10 wt%)	1357:1395	Improved ECM distribution in those constructs is associated with increased construct stiffness after chondrogenic differentiation, as compared to higher concentrated constructs (10 wt%), which only show pericellular matrix deposition.
32757263	7	82	theme	higher	1357:1362	arg1	%					1394:1394	10 wt%	1389:1394	10 wt%	1389:1394	Improved ECM distribution in those constructs is associated with increased construct stiffness after chondrogenic differentiation, as compared to higher concentrated constructs (10 wt%), which only show pericellular matrix deposition.
32757263	1	83	theme	cell-hydrogel	259:271	arg1	constructs					273:282	3D cell-hydrogel constructs	256:282	3D cell-hydrogel constructs with sufficient stability	256:308	In 3D bioprinting, bioinks with high concentrations of polymeric materials are frequently used to enable fabrication of 3D cell-hydrogel constructs with sufficient stability.
33130265	0	0	from	Laminaria	18:26	arg1	Galactofucan					0:11	Galactofucan	0:11	Galactofucan from Laminaria japonica	0:35	Galactofucan from Laminaria japonica is not degraded by the human digestive system but inhibits pancreatic lipase and modifies the intestinal microbiota.
33130265	3	1	theme	human	494:498	arg1	saliva					500:505	human saliva	494:505	human saliva	494:505	In the simulated digestion model for the human saliva and gastrointestinal tract, no obvious changes in the molecular weight or the reducing sugar content of CGF-3 were observed, indicating CGF-3 is resistant to the human digestive system.
33130265	1	2	theme	human	252:256	arg1	microbiota					238:247	intestinal microbiota	227:247	intestinal microbiota	227:247	The effects of galactofucan from Laminaria japonica on the digestion and intestinal microbiota of human were investigated in the present study.
33130265	1	2	theme	human	252:256	arg1	digestion					213:221	digestion	213:221	digestion	213:221	The effects of galactofucan from Laminaria japonica on the digestion and intestinal microbiota of human were investigated in the present study.
33130265	0	3	theme	digestive	66:74	arg1	system					76:81	the human digestive system	56:81	the human digestive system	56:81	Galactofucan from Laminaria japonica is not degraded by the human digestive system but inhibits pancreatic lipase and modifies the intestinal microbiota.
33130265	5	4	with	fermentation	868:879	arg1	microbiota					902:911	the human fecal microbiota	886:911	the human fecal microbiota	886:911	In the in vitro fermentation with the human fecal microbiota, CF did not change the total carbohydrate, reducing sugar and short chain fatty acids contents, which indicated CF was not utilized by the microbiota.
33130265	1	5	dep	digestion	213:221	arg1	the					209:211	the	209:211	the	209:211	The effects of galactofucan from Laminaria japonica on the digestion and intestinal microbiota of human were investigated in the present study.
33130265	1	6	from	effects	158:164	arg1	microbiota					238:247	intestinal microbiota	227:247	intestinal microbiota	227:247	The effects of galactofucan from Laminaria japonica on the digestion and intestinal microbiota of human were investigated in the present study.
33130265	1	6	from	effects	158:164	arg1	Laminaria					187:195	Laminaria	187:195	Laminaria	187:195	The effects of galactofucan from Laminaria japonica on the digestion and intestinal microbiota of human were investigated in the present study.
33130265	1	6	from	effects	158:164	arg1	digestion					213:221	digestion	213:221	digestion	213:221	The effects of galactofucan from Laminaria japonica on the digestion and intestinal microbiota of human were investigated in the present study.
33130265	7	7	theme	dietary	1216:1222	arg1	galactofucan					1224:1235	dietary galactofucan	1216:1235	dietary galactofucan	1216:1235	These findings shed a light on the better understanding of the impacts of dietary galactofucan on the digestion and intestinal microbiota.
33130265	5	8	theme	total	936:940	arg1	carbohydrate					942:953	the total carbohydrate	932:953	the total carbohydrate	932:953	In the in vitro fermentation with the human fecal microbiota, CF did not change the total carbohydrate, reducing sugar and short chain fatty acids contents, which indicated CF was not utilized by the microbiota.
33130265	3	9	theme	obvious	538:544	arg1	changes					546:552	no obvious changes	535:552	no obvious changes in the molecular weight or the reducing sugar content of CGF-3	535:615	In the simulated digestion model for the human saliva and gastrointestinal tract, no obvious changes in the molecular weight or the reducing sugar content of CGF-3 were observed, indicating CGF-3 is resistant to the human digestive system.
33130265	7	10	theme	galactofucan	1224:1235	arg1	impacts					1205:1211	the impacts	1201:1211	the impacts of dietary galactofucan	1201:1235	These findings shed a light on the better understanding of the impacts of dietary galactofucan on the digestion and intestinal microbiota.
33130265	2	11	theme	homogeneous	391:401	arg1	CGF-3					413:417	CGF-3	413:417	CGF-3	413:417	Crude fraction of the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3) were prepared and characterized.
33130265	2	11	theme	homogeneous	391:401	arg1	fraction					403:410	its molecular-weight homogeneous fraction	370:410	its molecular-weight homogeneous fraction (CGF-3)	370:418	Crude fraction of the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3) were prepared and characterized.
33130265	0	12	theme	pancreatic	96:105	arg1	lipase					107:112	pancreatic lipase	96:112	pancreatic lipase	96:112	Galactofucan from Laminaria japonica is not degraded by the human digestive system but inhibits pancreatic lipase and modifies the intestinal microbiota.
33130265	6	13	theme	CF	1125:1126	arg1	intervention					1128:1139	CF intervention	1125:1139	CF intervention	1125:1139	However, the microbiota composition was modulated greatly by CF intervention.
33130265	1	14	theme	galactofucan	169:180	arg1	effects					158:164	The effects	154:164	The effects of galactofucan from Laminaria japonica on the digestion and intestinal microbiota of human	154:256	The effects of galactofucan from Laminaria japonica on the digestion and intestinal microbiota of human were investigated in the present study.
33130265	5	15	theme	fatty	987:991	arg1	acids					993:997	short chain fatty acids	975:997	short chain fatty acids	975:997	In the in vitro fermentation with the human fecal microbiota, CF did not change the total carbohydrate, reducing sugar and short chain fatty acids contents, which indicated CF was not utilized by the microbiota.
33130265	2	16	from	japonica	352:359	arg1	fraction					304:311	Crude fraction	298:311	Crude fraction of the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3)	298:418	Crude fraction of the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3) were prepared and characterized.
33130265	2	16	from	japonica	352:359	arg1	polysaccharide					329:342	the sulfated polysaccharide	316:342	the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3)	316:418	Crude fraction of the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3) were prepared and characterized.
33130265	4	17	theme	sulfate	836:842	arg1	groups					844:849	its sulfate groups	832:849	its sulfate groups	832:849	Then CGF-3 did not affect the α-amylase activity while it dose-dependently inhibited the activity of pancreatic lipase partly depending on its sulfate groups.
33130265	3	18	theme	molecular	561:569	arg1	weight					571:576	the molecular weight	557:576	the molecular weight	557:576	In the simulated digestion model for the human saliva and gastrointestinal tract, no obvious changes in the molecular weight or the reducing sugar content of CGF-3 were observed, indicating CGF-3 is resistant to the human digestive system.
33130265	5	19	theme	in	859:860	arg1	fermentation					868:879	the in vitro fermentation	855:879	the in vitro fermentation with the human fecal microbiota	855:911	In the in vitro fermentation with the human fecal microbiota, CF did not change the total carbohydrate, reducing sugar and short chain fatty acids contents, which indicated CF was not utilized by the microbiota.
33130265	1	20	theme	present	283:289	arg1	study					291:295	the present study	279:295	the present study	279:295	The effects of galactofucan from Laminaria japonica on the digestion and intestinal microbiota of human were investigated in the present study.
33130265	4	21	theme	α-amylase	723:731	arg1	activity					733:740	the α-amylase activity	719:740	the α-amylase activity	719:740	Then CGF-3 did not affect the α-amylase activity while it dose-dependently inhibited the activity of pancreatic lipase partly depending on its sulfate groups.
33130265	5	22	theme	chain	981:985	arg1	acids					993:997	short chain fatty acids	975:997	short chain fatty acids	975:997	In the in vitro fermentation with the human fecal microbiota, CF did not change the total carbohydrate, reducing sugar and short chain fatty acids contents, which indicated CF was not utilized by the microbiota.
33130265	7	23	theme	intestinal	1258:1267	arg1	microbiota					1269:1278	intestinal microbiota	1258:1278	intestinal microbiota	1258:1278	These findings shed a light on the better understanding of the impacts of dietary galactofucan on the digestion and intestinal microbiota.
33130265	2	24	from	fraction	403:410	arg1	fraction					304:311	Crude fraction	298:311	Crude fraction of the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3)	298:418	Crude fraction of the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3) were prepared and characterized.
33130265	2	24	from	fraction	403:410	arg1	polysaccharide					329:342	the sulfated polysaccharide	316:342	the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3)	316:418	Crude fraction of the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3) were prepared and characterized.
33130265	5	25	dep	in	859:860	arg1	vitro					862:866	vitro	862:866	vitro	862:866	In the in vitro fermentation with the human fecal microbiota, CF did not change the total carbohydrate, reducing sugar and short chain fatty acids contents, which indicated CF was not utilized by the microbiota.
33130265	2	26	theme	Crude	298:302	arg1	fraction					304:311	Crude fraction	298:311	Crude fraction of the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3)	298:418	Crude fraction of the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3) were prepared and characterized.
33130265	3	27	theme	simulated	460:468	arg1	model					480:484	the simulated digestion model	456:484	the simulated digestion model for the human saliva and gastrointestinal tract	456:532	In the simulated digestion model for the human saliva and gastrointestinal tract, no obvious changes in the molecular weight or the reducing sugar content of CGF-3 were observed, indicating CGF-3 is resistant to the human digestive system.
33130265	5	28	theme	fecal	896:900	arg1	microbiota					902:911	the human fecal microbiota	886:911	the human fecal microbiota	886:911	In the in vitro fermentation with the human fecal microbiota, CF did not change the total carbohydrate, reducing sugar and short chain fatty acids contents, which indicated CF was not utilized by the microbiota.
33130265	0	29	theme	intestinal	131:140	arg1	microbiota					142:151	the intestinal microbiota	127:151	the intestinal microbiota	127:151	Galactofucan from Laminaria japonica is not degraded by the human digestive system but inhibits pancreatic lipase and modifies the intestinal microbiota.
33130265	6	30	theme	microbiota	1077:1086	arg1	composition					1088:1098	the microbiota composition	1073:1098	the microbiota composition	1073:1098	However, the microbiota composition was modulated greatly by CF intervention.
33130265	4	31	theme	lipase	805:810	arg1	activity					782:789	the activity	778:789	the activity of pancreatic lipase	778:810	Then CGF-3 did not affect the α-amylase activity while it dose-dependently inhibited the activity of pancreatic lipase partly depending on its sulfate groups.
33130265	3	32	theme	CGF-3	611:615	arg1	content					600:606	the reducing sugar content	581:606	the reducing sugar content of CGF-3	581:615	In the simulated digestion model for the human saliva and gastrointestinal tract, no obvious changes in the molecular weight or the reducing sugar content of CGF-3 were observed, indicating CGF-3 is resistant to the human digestive system.
33130265	3	32	theme	CGF-3	611:615	arg1	weight					571:576	the molecular weight	557:576	the molecular weight	557:576	In the simulated digestion model for the human saliva and gastrointestinal tract, no obvious changes in the molecular weight or the reducing sugar content of CGF-3 were observed, indicating CGF-3 is resistant to the human digestive system.
33130265	1	33	dep	Laminaria	187:195	arg1	japonica					197:204	Laminaria japonica	187:204	Laminaria japonica	187:204	The effects of galactofucan from Laminaria japonica on the digestion and intestinal microbiota of human were investigated in the present study.
33130265	5	34	dep	sugar	965:969	arg1	contents					999:1006	contents	999:1006	contents	999:1006	In the in vitro fermentation with the human fecal microbiota, CF did not change the total carbohydrate, reducing sugar and short chain fatty acids contents, which indicated CF was not utilized by the microbiota.
33130265	3	35	located	observed	622:629	arg1	model					480:484	the simulated digestion model	456:484	the simulated digestion model for the human saliva and gastrointestinal tract	456:532	In the simulated digestion model for the human saliva and gastrointestinal tract, no obvious changes in the molecular weight or the reducing sugar content of CGF-3 were observed, indicating CGF-3 is resistant to the human digestive system.
33130265	3	35	located	observed	622:629	arg2	changes					546:552	no obvious changes	535:552	no obvious changes in the molecular weight or the reducing sugar content of CGF-3	535:615	In the simulated digestion model for the human saliva and gastrointestinal tract, no obvious changes in the molecular weight or the reducing sugar content of CGF-3 were observed, indicating CGF-3 is resistant to the human digestive system.
33130265	5	36	used	utilized	1036:1043	arg2	CF					1025:1026	CF	1025:1026	CF	1025:1026	In the in vitro fermentation with the human fecal microbiota, CF did not change the total carbohydrate, reducing sugar and short chain fatty acids contents, which indicated CF was not utilized by the microbiota.
33130265	7	37	theme	better	1177:1182	arg1	understanding					1184:1196	the better understanding	1173:1196	the better understanding of the impacts of dietary galactofucan	1173:1235	These findings shed a light on the better understanding of the impacts of dietary galactofucan on the digestion and intestinal microbiota.
33130265	5	38	theme	human	890:894	arg1	microbiota					902:911	the human fecal microbiota	886:911	the human fecal microbiota	886:911	In the in vitro fermentation with the human fecal microbiota, CF did not change the total carbohydrate, reducing sugar and short chain fatty acids contents, which indicated CF was not utilized by the microbiota.
33130265	3	39	theme	human	669:673	arg1	system					685:690	the human digestive system	665:690	the human digestive system	665:690	In the simulated digestion model for the human saliva and gastrointestinal tract, no obvious changes in the molecular weight or the reducing sugar content of CGF-3 were observed, indicating CGF-3 is resistant to the human digestive system.
33130265	3	40	theme	digestion	470:478	arg1	model					480:484	the simulated digestion model	456:484	the simulated digestion model for the human saliva and gastrointestinal tract	456:532	In the simulated digestion model for the human saliva and gastrointestinal tract, no obvious changes in the molecular weight or the reducing sugar content of CGF-3 were observed, indicating CGF-3 is resistant to the human digestive system.
33130265	3	41	dep	saliva	500:505	arg1	the					490:492	the	490:492	the	490:492	In the simulated digestion model for the human saliva and gastrointestinal tract, no obvious changes in the molecular weight or the reducing sugar content of CGF-3 were observed, indicating CGF-3 is resistant to the human digestive system.
33130265	3	42	from	changes	546:552	arg1	content					600:606	the reducing sugar content	581:606	the reducing sugar content of CGF-3	581:615	In the simulated digestion model for the human saliva and gastrointestinal tract, no obvious changes in the molecular weight or the reducing sugar content of CGF-3 were observed, indicating CGF-3 is resistant to the human digestive system.
33130265	3	42	from	changes	546:552	arg1	weight					571:576	the molecular weight	557:576	the molecular weight	557:576	In the simulated digestion model for the human saliva and gastrointestinal tract, no obvious changes in the molecular weight or the reducing sugar content of CGF-3 were observed, indicating CGF-3 is resistant to the human digestive system.
33130265	4	43	theme	pancreatic	794:803	arg1	lipase					805:810	pancreatic lipase	794:810	pancreatic lipase	794:810	Then CGF-3 did not affect the α-amylase activity while it dose-dependently inhibited the activity of pancreatic lipase partly depending on its sulfate groups.
33130265	3	44	theme	reducing	585:592	arg1	content					600:606	the reducing sugar content	581:606	the reducing sugar content of CGF-3	581:615	In the simulated digestion model for the human saliva and gastrointestinal tract, no obvious changes in the molecular weight or the reducing sugar content of CGF-3 were observed, indicating CGF-3 is resistant to the human digestive system.
33130265	2	45	theme	polysaccharide	329:342	arg1	fraction					304:311	Crude fraction	298:311	Crude fraction of the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3)	298:418	Crude fraction of the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3) were prepared and characterized.
33130265	0	46	dep	Laminaria	18:26	arg1	japonica					28:35	Laminaria japonica	18:35	Laminaria japonica	18:35	Galactofucan from Laminaria japonica is not degraded by the human digestive system but inhibits pancreatic lipase and modifies the intestinal microbiota.
33130265	2	47	theme	sulfated	320:327	arg1	polysaccharide					329:342	the sulfated polysaccharide	316:342	the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3)	316:418	Crude fraction of the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3) were prepared and characterized.
33130265	1	48	theme	intestinal	227:236	arg1	microbiota					238:247	intestinal microbiota	227:247	intestinal microbiota	227:247	The effects of galactofucan from Laminaria japonica on the digestion and intestinal microbiota of human were investigated in the present study.
33130265	0	49	theme	human	60:64	arg1	system					76:81	the human digestive system	56:81	the human digestive system	56:81	Galactofucan from Laminaria japonica is not degraded by the human digestive system but inhibits pancreatic lipase and modifies the intestinal microbiota.
33130265	3	50	theme	sugar	594:598	arg1	content					600:606	the reducing sugar content	581:606	the reducing sugar content of CGF-3	581:615	In the simulated digestion model for the human saliva and gastrointestinal tract, no obvious changes in the molecular weight or the reducing sugar content of CGF-3 were observed, indicating CGF-3 is resistant to the human digestive system.
33130265	1	51	from	Laminaria	187:195	arg1	galactofucan					169:180	galactofucan	169:180	galactofucan from Laminaria japonica	169:204	The effects of galactofucan from Laminaria japonica on the digestion and intestinal microbiota of human were investigated in the present study.
33130265	1	51	from	Laminaria	187:195	arg1	effects					158:164	The effects	154:164	The effects of galactofucan from Laminaria japonica on the digestion and intestinal microbiota of human	154:256	The effects of galactofucan from Laminaria japonica on the digestion and intestinal microbiota of human were investigated in the present study.
33130265	7	52	theme	impacts	1205:1211	arg1	understanding					1184:1196	the better understanding	1173:1196	the better understanding of the impacts of dietary galactofucan	1173:1235	These findings shed a light on the better understanding of the impacts of dietary galactofucan on the digestion and intestinal microbiota.
33130265	2	53	theme	molecular-weight	374:389	arg1	CGF-3					413:417	CGF-3	413:417	CGF-3	413:417	Crude fraction of the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3) were prepared and characterized.
33130265	2	53	theme	molecular-weight	374:389	arg1	fraction					403:410	its molecular-weight homogeneous fraction	370:410	its molecular-weight homogeneous fraction (CGF-3)	370:418	Crude fraction of the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3) were prepared and characterized.
33130265	3	54	theme	digestive	675:683	arg1	system					685:690	the human digestive system	665:690	the human digestive system	665:690	In the simulated digestion model for the human saliva and gastrointestinal tract, no obvious changes in the molecular weight or the reducing sugar content of CGF-3 were observed, indicating CGF-3 is resistant to the human digestive system.
33130265	5	55	theme	short	975:979	arg1	acids					993:997	short chain fatty acids	975:997	short chain fatty acids	975:997	In the in vitro fermentation with the human fecal microbiota, CF did not change the total carbohydrate, reducing sugar and short chain fatty acids contents, which indicated CF was not utilized by the microbiota.
33130265	3	56	theme	gastrointestinal	511:526	arg1	tract					528:532	gastrointestinal tract	511:532	gastrointestinal tract	511:532	In the simulated digestion model for the human saliva and gastrointestinal tract, no obvious changes in the molecular weight or the reducing sugar content of CGF-3 were observed, indicating CGF-3 is resistant to the human digestive system.
33130265	2	57	from	fraction	304:311	arg1	CF					362:363	CF	362:363	CF	362:363	Crude fraction of the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3) were prepared and characterized.
33130265	2	57	from	fraction	304:311	arg1	CGF-3					413:417	CGF-3	413:417	CGF-3	413:417	Crude fraction of the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3) were prepared and characterized.
33130265	2	57	from	fraction	304:311	arg1	japonica					352:359	L. japonica	349:359	L. japonica (CF)	349:364	Crude fraction of the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3) were prepared and characterized.
33130265	2	57	from	fraction	304:311	arg1	fraction					403:410	its molecular-weight homogeneous fraction	370:410	its molecular-weight homogeneous fraction (CGF-3)	370:418	Crude fraction of the sulfated polysaccharide from L. japonica (CF) and its molecular-weight homogeneous fraction (CGF-3) were prepared and characterized.
34075195	9	0	theme	direct	1334:1339	arg1	system					1350:1355	direct delivery system	1334:1355	direct delivery system for the oral application of protein derived therapies	1334:1409	The results of this study are important for application of lysosomes as therapeutic agents, and also it was confirmed that alginate oligosaccharides have potential as direct delivery system for the oral application of protein derived therapies.
34075195	5	1	theme	lysosome-alginate	642:658	arg1	form					687:690	lysosome-alginate oligosaccharides composite form	642:690	lysosome-alginate oligosaccharides composite form	642:690	However, when lysosome-alginate oligosaccharides composite form was used, antimicrobial activity of lysozyme was maintained.
34075195	9	2	contain	have	1316:1319	arg2	potential					1321:1329	potential	1321:1329	potential	1321:1329	The results of this study are important for application of lysosomes as therapeutic agents, and also it was confirmed that alginate oligosaccharides have potential as direct delivery system for the oral application of protein derived therapies.
34075195	9	2	contain	have	1316:1319	arg1	oligosaccharides					1299:1314	alginate oligosaccharides	1290:1314	alginate oligosaccharides	1290:1314	The results of this study are important for application of lysosomes as therapeutic agents, and also it was confirmed that alginate oligosaccharides have potential as direct delivery system for the oral application of protein derived therapies.
34075195	9	3	theme	delivery	1341:1348	arg1	system					1350:1355	direct delivery system	1334:1355	direct delivery system for the oral application of protein derived therapies	1334:1409	The results of this study are important for application of lysosomes as therapeutic agents, and also it was confirmed that alginate oligosaccharides have potential as direct delivery system for the oral application of protein derived therapies.
34075195	5	4	theme	oligosaccharides	660:675	arg1	form					687:690	lysosome-alginate oligosaccharides composite form	642:690	lysosome-alginate oligosaccharides composite form	642:690	However, when lysosome-alginate oligosaccharides composite form was used, antimicrobial activity of lysozyme was maintained.
34075195	8	5	theme	lysosomes	1006:1014	arg1	analysis					985:992	SDS-polyacrylamide gel electrophoresis analysis	946:992	SDS-polyacrylamide gel electrophoresis analysis of released lysosomes	946:1014	SDS-polyacrylamide gel electrophoresis analysis of released lysosomes revealed that alginate oligosaccharide could effectively protect the lysosome from degradation or hydrolysis under acidic conditions for at least 2 h.
34075195	4	6	dep	pH.	568:570	arg1	reduced					619:625	reduced	619:625	was also remarkably reduced	599:625	When lysosome were used as an independent drug, the activity of protein was lost due to influence of low pH. Its antibacterial activity was also remarkably reduced.
34075195	4	7	theme	pH.	568:570	arg1	influence					551:559	influence	551:559	influence of low pH. Its antibacterial activity was also remarkably reduced	551:625	When lysosome were used as an independent drug, the activity of protein was lost due to influence of low pH. Its antibacterial activity was also remarkably reduced.
34075195	5	8	theme	composite	677:685	arg1	form					687:690	lysosome-alginate oligosaccharides composite form	642:690	lysosome-alginate oligosaccharides composite form	642:690	However, when lysosome-alginate oligosaccharides composite form was used, antimicrobial activity of lysozyme was maintained.
34075195	8	9	theme	released	997:1004	arg1	lysosomes					1006:1014	released lysosomes	997:1014	released lysosomes	997:1014	SDS-polyacrylamide gel electrophoresis analysis of released lysosomes revealed that alginate oligosaccharide could effectively protect the lysosome from degradation or hydrolysis under acidic conditions for at least 2 h.
34075195	7	10	theme	alginate	874:881	arg1	oligosaccharides					883:898	alginate oligosaccharides	874:898	alginate oligosaccharides	874:898	When the pH was increased, alginate oligosaccharides were dissolved and the lysosome was released.
34075195	8	11	theme	electrophoresis	969:983	arg1	analysis					985:992	SDS-polyacrylamide gel electrophoresis analysis	946:992	SDS-polyacrylamide gel electrophoresis analysis of released lysosomes	946:1014	SDS-polyacrylamide gel electrophoresis analysis of released lysosomes revealed that alginate oligosaccharide could effectively protect the lysosome from degradation or hydrolysis under acidic conditions for at least 2 h.
34075195	9	12	theme	derived	1393:1399	arg1	therapies					1401:1409	protein derived therapies	1385:1409	protein derived therapies	1385:1409	The results of this study are important for application of lysosomes as therapeutic agents, and also it was confirmed that alginate oligosaccharides have potential as direct delivery system for the oral application of protein derived therapies.
34075195	4	13	theme	antibacterial	576:588	arg1	activity					590:597	Its antibacterial activity	572:597	Its antibacterial activity	572:597	When lysosome were used as an independent drug, the activity of protein was lost due to influence of low pH. Its antibacterial activity was also remarkably reduced.
34075195	1	14	theme	study	109:113	arg1	objective					91:99	The objective	87:99	The objective of this study	87:113	The objective of this study was to report that lysosome extracted from egg white could be used as a drug through oral administration for treating diseases by using pH sensitive alginate oligosaccharides.
34075195	6	15	theme	alginate	796:803	arg1	oligosaccharides					805:820	alginate oligosaccharides	796:820	alginate oligosaccharides	796:820	At low pH, a gel-like matrix was formed by alginate oligosaccharides to protect the lysosome.
34075195	3	16	theme	form	457:460	arg1	availability					425:436	the availability	421:436	the availability of the oral dosage form	421:460	The dissolution test confirmed the availability of the oral dosage form.
34075195	9	17	theme	oral	1365:1368	arg1	application					1370:1380	the oral application	1361:1380	the oral application of protein derived therapies	1361:1409	The results of this study are important for application of lysosomes as therapeutic agents, and also it was confirmed that alginate oligosaccharides have potential as direct delivery system for the oral application of protein derived therapies.
34075195	1	18	theme	white	162:166	arg1	egg					158:160	egg white	158:166	egg white	158:166	The objective of this study was to report that lysosome extracted from egg white could be used as a drug through oral administration for treating diseases by using pH sensitive alginate oligosaccharides.
34075195	4	19	theme	independent	493:503	arg1	lysosome					468:475	lysosome	468:475	lysosome	468:475	When lysosome were used as an independent drug, the activity of protein was lost due to influence of low pH. Its antibacterial activity was also remarkably reduced.
34075195	4	19	theme	independent	493:503	arg1	drug					505:508	an independent drug	490:508	an independent drug	490:508	When lysosome were used as an independent drug, the activity of protein was lost due to influence of low pH. Its antibacterial activity was also remarkably reduced.
34075195	8	20	theme	alginate	1030:1037	arg1	oligosaccharide					1039:1053	alginate oligosaccharide	1030:1053	alginate oligosaccharide	1030:1053	SDS-polyacrylamide gel electrophoresis analysis of released lysosomes revealed that alginate oligosaccharide could effectively protect the lysosome from degradation or hydrolysis under acidic conditions for at least 2 h.
34075195	3	21	theme	oral	445:448	arg1	form					457:460	the oral dosage form	441:460	the oral dosage form	441:460	The dissolution test confirmed the availability of the oral dosage form.
34075195	0	22	theme	Alginate	0:7	arg1	oligosaccharides					9:24	Alginate oligosaccharides	0:24	Alginate oligosaccharides	0:24	Alginate oligosaccharides can maintain activities of lysosomes under low pH condition.
34075195	2	23	theme	oligosaccharides	309:324	arg1	composite					326:334	Lysosome-alginate oligosaccharides composite	291:334	Lysosome-alginate oligosaccharides composite	291:334	Lysosome-alginate oligosaccharides composite were formulated for oral administration of lysosomes.
34075195	3	24	theme	dosage	450:455	arg1	form					457:460	the oral dosage form	441:460	the oral dosage form	441:460	The dissolution test confirmed the availability of the oral dosage form.
34075195	5	25	used	used	696:699	arg2	form					687:690	lysosome-alginate oligosaccharides composite form	642:690	lysosome-alginate oligosaccharides composite form	642:690	However, when lysosome-alginate oligosaccharides composite form was used, antimicrobial activity of lysozyme was maintained.
34075195	1	26	theme	sensitive	254:262	arg1	oligosaccharides					273:288	pH sensitive alginate oligosaccharides	251:288	pH sensitive alginate oligosaccharides	251:288	The objective of this study was to report that lysosome extracted from egg white could be used as a drug through oral administration for treating diseases by using pH sensitive alginate oligosaccharides.
34075195	9	27	theme	therapeutic	1239:1249	arg1	agents					1251:1256	therapeutic agents	1239:1256	therapeutic agents	1239:1256	The results of this study are important for application of lysosomes as therapeutic agents, and also it was confirmed that alginate oligosaccharides have potential as direct delivery system for the oral application of protein derived therapies.
34075195	2	28	theme	Lysosome-alginate	291:307	arg1	composite					326:334	Lysosome-alginate oligosaccharides composite	291:334	Lysosome-alginate oligosaccharides composite	291:334	Lysosome-alginate oligosaccharides composite were formulated for oral administration of lysosomes.
34075195	3	29	theme	dissolution	394:404	arg1	test					406:409	The dissolution test	390:409	The dissolution test	390:409	The dissolution test confirmed the availability of the oral dosage form.
34075195	1	30	theme	alginate	264:271	arg1	oligosaccharides					273:288	pH sensitive alginate oligosaccharides	251:288	pH sensitive alginate oligosaccharides	251:288	The objective of this study was to report that lysosome extracted from egg white could be used as a drug through oral administration for treating diseases by using pH sensitive alginate oligosaccharides.
34075195	8	31	theme	acidic	1131:1136	arg1	conditions					1138:1147	acidic conditions	1131:1147	acidic conditions for at least 2 h	1131:1164	SDS-polyacrylamide gel electrophoresis analysis of released lysosomes revealed that alginate oligosaccharide could effectively protect the lysosome from degradation or hydrolysis under acidic conditions for at least 2 h.
34075195	9	32	link	derived	1393:1399	arg1	therapies					1401:1409	protein derived therapies	1385:1409	protein derived therapies	1385:1409	The results of this study are important for application of lysosomes as therapeutic agents, and also it was confirmed that alginate oligosaccharides have potential as direct delivery system for the oral application of protein derived therapies.
34075195	9	33	theme	therapies	1401:1409	arg1	application					1370:1380	the oral application	1361:1380	the oral application of protein derived therapies	1361:1409	The results of this study are important for application of lysosomes as therapeutic agents, and also it was confirmed that alginate oligosaccharides have potential as direct delivery system for the oral application of protein derived therapies.
34075195	5	34	theme	lysozyme	728:735	arg1	activity					716:723	antimicrobial activity	702:723	antimicrobial activity of lysozyme	702:735	However, when lysosome-alginate oligosaccharides composite form was used, antimicrobial activity of lysozyme was maintained.
34075195	9	35	theme	study	1187:1191	arg1	important					1197:1205	important	1197:1205	important	1197:1205	The results of this study are important for application of lysosomes as therapeutic agents, and also it was confirmed that alginate oligosaccharides have potential as direct delivery system for the oral application of protein derived therapies.
34075195	9	35	theme	study	1187:1191	arg1	results					1171:1177	The results	1167:1177	The results of this study	1167:1191	The results of this study are important for application of lysosomes as therapeutic agents, and also it was confirmed that alginate oligosaccharides have potential as direct delivery system for the oral application of protein derived therapies.
34075195	6	36	theme	gel-like	766:773	arg1	matrix					775:780	a gel-like matrix	764:780	a gel-like matrix	764:780	At low pH, a gel-like matrix was formed by alginate oligosaccharides to protect the lysosome.
34075195	4	37	theme	protein	527:533	arg1	activity					515:522	the activity	511:522	the activity of protein	511:533	When lysosome were used as an independent drug, the activity of protein was lost due to influence of low pH. Its antibacterial activity was also remarkably reduced.
34075195	0	38	theme	low	69:71	arg1	condition					76:84	low pH condition	69:84	low pH condition	69:84	Alginate oligosaccharides can maintain activities of lysosomes under low pH condition.
34075195	2	39	theme	oral	356:359	arg1	administration					361:374	oral administration	356:374	oral administration of lysosomes	356:387	Lysosome-alginate oligosaccharides composite were formulated for oral administration of lysosomes.
34075195	0	40	theme	lysosomes	53:61	arg1	activities					39:48	activities	39:48	activities of lysosomes	39:61	Alginate oligosaccharides can maintain activities of lysosomes under low pH condition.
34075195	9	41	theme	alginate	1290:1297	arg1	oligosaccharides					1299:1314	alginate oligosaccharides	1290:1314	alginate oligosaccharides	1290:1314	The results of this study are important for application of lysosomes as therapeutic agents, and also it was confirmed that alginate oligosaccharides have potential as direct delivery system for the oral application of protein derived therapies.
34075195	1	42	used	used	177:180	arg2	drug					187:190	a drug	185:190	a drug	185:190	The objective of this study was to report that lysosome extracted from egg white could be used as a drug through oral administration for treating diseases by using pH sensitive alginate oligosaccharides.
34075195	1	42	used	used	177:180	arg2	lysosome					134:141	lysosome	134:141	lysosome extracted from egg white	134:166	The objective of this study was to report that lysosome extracted from egg white could be used as a drug through oral administration for treating diseases by using pH sensitive alginate oligosaccharides.
34075195	8	43	theme	SDS-polyacrylamide	946:963	arg1	electrophoresis					969:983	SDS-polyacrylamide gel electrophoresis	946:983	SDS-polyacrylamide gel electrophoresis analysis of released lysosomes	946:1014	SDS-polyacrylamide gel electrophoresis analysis of released lysosomes revealed that alginate oligosaccharide could effectively protect the lysosome from degradation or hydrolysis under acidic conditions for at least 2 h.
34075195	9	44	theme	lysosomes	1226:1234	arg1	application					1211:1221	application	1211:1221	application of lysosomes as therapeutic agents	1211:1256	The results of this study are important for application of lysosomes as therapeutic agents, and also it was confirmed that alginate oligosaccharides have potential as direct delivery system for the oral application of protein derived therapies.
34075195	5	45	theme	antimicrobial	702:714	arg1	activity					716:723	antimicrobial activity	702:723	antimicrobial activity of lysozyme	702:735	However, when lysosome-alginate oligosaccharides composite form was used, antimicrobial activity of lysozyme was maintained.
34075195	6	46	theme	low	756:758	arg1	pH					760:761	low pH	756:761	low pH	756:761	At low pH, a gel-like matrix was formed by alginate oligosaccharides to protect the lysosome.
34075195	1	47	theme	oral	200:203	arg1	administration					205:218	oral administration	200:218	oral administration for treating diseases by using pH sensitive alginate oligosaccharides	200:288	The objective of this study was to report that lysosome extracted from egg white could be used as a drug through oral administration for treating diseases by using pH sensitive alginate oligosaccharides.
34075195	0	48	theme	pH	73:74	arg1	condition					76:84	low pH condition	69:84	low pH condition	69:84	Alginate oligosaccharides can maintain activities of lysosomes under low pH condition.
34075195	8	49	theme	gel	965:967	arg1	electrophoresis					969:983	SDS-polyacrylamide gel electrophoresis	946:983	SDS-polyacrylamide gel electrophoresis analysis of released lysosomes	946:1014	SDS-polyacrylamide gel electrophoresis analysis of released lysosomes revealed that alginate oligosaccharide could effectively protect the lysosome from degradation or hydrolysis under acidic conditions for at least 2 h.
34075195	4	50	used	used	482:485	arg2	lysosome					468:475	lysosome	468:475	lysosome	468:475	When lysosome were used as an independent drug, the activity of protein was lost due to influence of low pH. Its antibacterial activity was also remarkably reduced.
34075195	4	50	used	used	482:485	arg2	drug					505:508	an independent drug	490:508	an independent drug	490:508	When lysosome were used as an independent drug, the activity of protein was lost due to influence of low pH. Its antibacterial activity was also remarkably reduced.
34075195	9	51	dep	derived	1393:1399	arg1	protein					1385:1391	protein	1385:1391	protein	1385:1391	The results of this study are important for application of lysosomes as therapeutic agents, and also it was confirmed that alginate oligosaccharides have potential as direct delivery system for the oral application of protein derived therapies.
34075195	2	52	theme	lysosomes	379:387	arg1	administration					361:374	oral administration	356:374	oral administration of lysosomes	356:387	Lysosome-alginate oligosaccharides composite were formulated for oral administration of lysosomes.
31616929	1	0	theme	human	151:155	arg1	condition					136:144	the health condition	125:144	the health condition of a human being	125:161	Urinary glycosaminoglycans (GAGs) can reflect the health condition of a human being, and the GAGs composition can be directly related to various diseases.
31616929	4	1	theme	total	541:545	arg1	content					581:587	The total creatinine-normalized urinary GAG content	537:587	The total creatinine-normalized urinary GAG content	537:587	The total creatinine-normalized urinary GAG content, molecular weight distribution and disaccharide compositions were determined.
31616929	0	2	from	analysis	11:18	arg1	subjects					69:76	healthy human subjects	55:76	healthy human subjects	55:76	Structural analysis of urinary glycosaminoglycans from healthy human subjects.
31616929	2	3	theme	healthy	328:334	arg1	individuals					336:346	healthy individuals	328:346	healthy individuals	328:346	In order to effectively utilize such information, a detailed understanding of urinary GAGs in healthy individuals can provide insight into the levels and structures of human urinary GAGs.
31616929	2	4	from	understanding	295:307	arg1	individuals					336:346	healthy individuals	328:346	healthy individuals	328:346	In order to effectively utilize such information, a detailed understanding of urinary GAGs in healthy individuals can provide insight into the levels and structures of human urinary GAGs.
31616929	6	5	theme	GAG	888:890	arg1	oligosaccharides					892:907	10 GAG oligosaccharides	885:907	10 GAG oligosaccharides representing the majority of human urinary GAGs	885:955	The structures of 10 GAG oligosaccharides representing the majority of human urinary GAGs were determined.
31616929	4	6	theme	urinary	569:575	arg1	content					581:587	The total creatinine-normalized urinary GAG content	537:587	The total creatinine-normalized urinary GAG content	537:587	The total creatinine-normalized urinary GAG content, molecular weight distribution and disaccharide compositions were determined.
31616929	3	7	theme	adults	512:517	arg1	females					501:507	females	501:507	females	501:507	In this study, urinary GAGs were collected and purified from healthy males and females of adults and young adults.
31616929	3	7	theme	adults	512:517	arg1	males					491:495	males	491:495	males	491:495	In this study, urinary GAGs were collected and purified from healthy males and females of adults and young adults.
31616929	2	8	theme	such	266:269	arg1	information					271:281	such information	266:281	such information	266:281	In order to effectively utilize such information, a detailed understanding of urinary GAGs in healthy individuals can provide insight into the levels and structures of human urinary GAGs.
31616929	4	9	theme	creatinine-normalized	547:567	arg1	content					581:587	The total creatinine-normalized urinary GAG content	537:587	The total creatinine-normalized urinary GAG content	537:587	The total creatinine-normalized urinary GAG content, molecular weight distribution and disaccharide compositions were determined.
31616929	5	10	theme	healthy	823:829	arg1	GAGs					845:848	healthy human urinary GAGs	823:848	healthy human urinary GAGs	823:848	Using capillary zone electrophoresis (CZE)-mass spectrometry (MS) and CZE-MS/MS relying on negative electron transfer dissociation, the major components of healthy human urinary GAGs were determined.
31616929	4	11	theme	weight	600:605	arg1	distribution					607:618	molecular weight distribution	590:618	molecular weight distribution	590:618	The total creatinine-normalized urinary GAG content, molecular weight distribution and disaccharide compositions were determined.
31616929	1	12	theme	Urinary	79:85	arg1	GAGs					107:110	GAGs	107:110	GAGs	107:110	Urinary glycosaminoglycans (GAGs) can reflect the health condition of a human being, and the GAGs composition can be directly related to various diseases.
31616929	1	12	theme	Urinary	79:85	arg1	glycosaminoglycans					87:104	Urinary glycosaminoglycans	79:104	Urinary glycosaminoglycans (GAGs)	79:111	Urinary glycosaminoglycans (GAGs) can reflect the health condition of a human being, and the GAGs composition can be directly related to various diseases.
31616929	5	13	theme	electron	767:774	arg1	dissociation					785:796	negative electron transfer dissociation	758:796	negative electron transfer dissociation	758:796	Using capillary zone electrophoresis (CZE)-mass spectrometry (MS) and CZE-MS/MS relying on negative electron transfer dissociation, the major components of healthy human urinary GAGs were determined.
31616929	4	14	theme	GAG	577:579	arg1	content					581:587	The total creatinine-normalized urinary GAG content	537:587	The total creatinine-normalized urinary GAG content	537:587	The total creatinine-normalized urinary GAG content, molecular weight distribution and disaccharide compositions were determined.
31616929	3	15	theme	adults	529:534	arg1	females					501:507	females	501:507	females	501:507	In this study, urinary GAGs were collected and purified from healthy males and females of adults and young adults.
31616929	3	15	theme	adults	529:534	arg1	males					491:495	males	491:495	males	491:495	In this study, urinary GAGs were collected and purified from healthy males and females of adults and young adults.
31616929	0	16	theme	Structural	0:9	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of urinary glycosaminoglycans from healthy human subjects	0:76	Structural analysis of urinary glycosaminoglycans from healthy human subjects.
31616929	5	17	theme	GAGs	845:848	arg1	components					809:818	the major components	799:818	the major components of healthy human urinary GAGs	799:848	Using capillary zone electrophoresis (CZE)-mass spectrometry (MS) and CZE-MS/MS relying on negative electron transfer dissociation, the major components of healthy human urinary GAGs were determined.
31616929	2	18	theme	detailed	286:293	arg1	understanding					295:307	a detailed understanding	284:307	a detailed understanding of urinary GAGs in healthy individuals	284:346	In order to effectively utilize such information, a detailed understanding of urinary GAGs in healthy individuals can provide insight into the levels and structures of human urinary GAGs.
31616929	1	19	theme	GAGs	172:175	arg1	related					205:211	related	205:211	related	205:211	Urinary glycosaminoglycans (GAGs) can reflect the health condition of a human being, and the GAGs composition can be directly related to various diseases.
31616929	1	19	theme	GAGs	172:175	arg1	composition					177:187	the GAGs composition	168:187	the GAGs composition	168:187	Urinary glycosaminoglycans (GAGs) can reflect the health condition of a human being, and the GAGs composition can be directly related to various diseases.
31616929	0	20	theme	urinary	23:29	arg1	glycosaminoglycans					31:48	urinary glycosaminoglycans	23:48	urinary glycosaminoglycans from healthy human subjects	23:76	Structural analysis of urinary glycosaminoglycans from healthy human subjects.
31616929	2	21	theme	human	402:406	arg1	GAGs					416:419	human urinary GAGs	402:419	human urinary GAGs	402:419	In order to effectively utilize such information, a detailed understanding of urinary GAGs in healthy individuals can provide insight into the levels and structures of human urinary GAGs.
31616929	2	22	theme	urinary	408:414	arg1	GAGs					416:419	human urinary GAGs	402:419	human urinary GAGs	402:419	In order to effectively utilize such information, a detailed understanding of urinary GAGs in healthy individuals can provide insight into the levels and structures of human urinary GAGs.
31616929	3	23	theme	young	523:527	arg1	adults					529:534	young adults	523:534	young adults	523:534	In this study, urinary GAGs were collected and purified from healthy males and females of adults and young adults.
31616929	3	24	theme	urinary	437:443	arg1	GAGs					445:448	urinary GAGs	437:448	urinary GAGs	437:448	In this study, urinary GAGs were collected and purified from healthy males and females of adults and young adults.
31616929	5	25	theme	negative	758:765	arg1	dissociation					785:796	negative electron transfer dissociation	758:796	negative electron transfer dissociation	758:796	Using capillary zone electrophoresis (CZE)-mass spectrometry (MS) and CZE-MS/MS relying on negative electron transfer dissociation, the major components of healthy human urinary GAGs were determined.
31616929	6	26	theme	GAGs	952:955	arg1	majority					926:933	the majority	922:933	the majority of human urinary GAGs	922:955	The structures of 10 GAG oligosaccharides representing the majority of human urinary GAGs were determined.
31616929	5	27	theme	human	831:835	arg1	GAGs					845:848	healthy human urinary GAGs	823:848	healthy human urinary GAGs	823:848	Using capillary zone electrophoresis (CZE)-mass spectrometry (MS) and CZE-MS/MS relying on negative electron transfer dissociation, the major components of healthy human urinary GAGs were determined.
31616929	4	28	theme	disaccharide	624:635	arg1	compositions					637:648	disaccharide compositions	624:648	disaccharide compositions	624:648	The total creatinine-normalized urinary GAG content, molecular weight distribution and disaccharide compositions were determined.
31616929	0	29	theme	glycosaminoglycans	31:48	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of urinary glycosaminoglycans from healthy human subjects	0:76	Structural analysis of urinary glycosaminoglycans from healthy human subjects.
31616929	6	30	theme	urinary	944:950	arg1	GAGs					952:955	human urinary GAGs	938:955	human urinary GAGs	938:955	The structures of 10 GAG oligosaccharides representing the majority of human urinary GAGs were determined.
31616929	5	31	theme	-mass	709:713	arg1	MS					729:730	MS	729:730	MS	729:730	Using capillary zone electrophoresis (CZE)-mass spectrometry (MS) and CZE-MS/MS relying on negative electron transfer dissociation, the major components of healthy human urinary GAGs were determined.
31616929	5	31	theme	-mass	709:713	arg1	spectrometry					715:726	capillary zone electrophoresis (CZE)-mass spectrometry	673:726	capillary zone electrophoresis (CZE)-mass spectrometry (MS)	673:731	Using capillary zone electrophoresis (CZE)-mass spectrometry (MS) and CZE-MS/MS relying on negative electron transfer dissociation, the major components of healthy human urinary GAGs were determined.
31616929	2	32	theme	GAGs	416:419	arg1	structures					388:397	structures	388:397	structures	388:397	In order to effectively utilize such information, a detailed understanding of urinary GAGs in healthy individuals can provide insight into the levels and structures of human urinary GAGs.
31616929	2	32	theme	GAGs	416:419	arg1	levels					377:382	levels	377:382	levels	377:382	In order to effectively utilize such information, a detailed understanding of urinary GAGs in healthy individuals can provide insight into the levels and structures of human urinary GAGs.
31616929	5	33	theme	major	803:807	arg1	components					809:818	the major components	799:818	the major components of healthy human urinary GAGs	799:848	Using capillary zone electrophoresis (CZE)-mass spectrometry (MS) and CZE-MS/MS relying on negative electron transfer dissociation, the major components of healthy human urinary GAGs were determined.
31616929	0	34	theme	human	63:67	arg1	subjects					69:76	healthy human subjects	55:76	healthy human subjects	55:76	Structural analysis of urinary glycosaminoglycans from healthy human subjects.
31616929	6	35	theme	human	938:942	arg1	GAGs					952:955	human urinary GAGs	938:955	human urinary GAGs	938:955	The structures of 10 GAG oligosaccharides representing the majority of human urinary GAGs were determined.
31616929	0	36	from	subjects	69:76	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of urinary glycosaminoglycans from healthy human subjects	0:76	Structural analysis of urinary glycosaminoglycans from healthy human subjects.
31616929	0	36	from	subjects	69:76	arg1	glycosaminoglycans					31:48	urinary glycosaminoglycans	23:48	urinary glycosaminoglycans from healthy human subjects	23:76	Structural analysis of urinary glycosaminoglycans from healthy human subjects.
31616929	0	37	theme	healthy	55:61	arg1	subjects					69:76	healthy human subjects	55:76	healthy human subjects	55:76	Structural analysis of urinary glycosaminoglycans from healthy human subjects.
31616929	5	38	theme	capillary	673:681	arg1	CZE					705:707	CZE	705:707	CZE	705:707	Using capillary zone electrophoresis (CZE)-mass spectrometry (MS) and CZE-MS/MS relying on negative electron transfer dissociation, the major components of healthy human urinary GAGs were determined.
31616929	5	38	theme	capillary	673:681	arg1	electrophoresis					688:702	capillary zone electrophoresis	673:702	capillary zone electrophoresis (CZE)-mass spectrometry (MS)	673:731	Using capillary zone electrophoresis (CZE)-mass spectrometry (MS) and CZE-MS/MS relying on negative electron transfer dissociation, the major components of healthy human urinary GAGs were determined.
31616929	6	39	theme	oligosaccharides	892:907	arg1	structures					871:880	The structures	867:880	The structures of 10 GAG oligosaccharides representing the majority of human urinary GAGs	867:955	The structures of 10 GAG oligosaccharides representing the majority of human urinary GAGs were determined.
31616929	2	40	theme	GAGs	320:323	arg1	understanding					295:307	a detailed understanding	284:307	a detailed understanding of urinary GAGs in healthy individuals	284:346	In order to effectively utilize such information, a detailed understanding of urinary GAGs in healthy individuals can provide insight into the levels and structures of human urinary GAGs.
31616929	5	41	theme	zone	683:686	arg1	CZE					705:707	CZE	705:707	CZE	705:707	Using capillary zone electrophoresis (CZE)-mass spectrometry (MS) and CZE-MS/MS relying on negative electron transfer dissociation, the major components of healthy human urinary GAGs were determined.
31616929	5	41	theme	zone	683:686	arg1	electrophoresis					688:702	capillary zone electrophoresis	673:702	capillary zone electrophoresis (CZE)-mass spectrometry (MS)	673:731	Using capillary zone electrophoresis (CZE)-mass spectrometry (MS) and CZE-MS/MS relying on negative electron transfer dissociation, the major components of healthy human urinary GAGs were determined.
31616929	1	42	theme	health	129:134	arg1	condition					136:144	the health condition	125:144	the health condition of a human being	125:161	Urinary glycosaminoglycans (GAGs) can reflect the health condition of a human being, and the GAGs composition can be directly related to various diseases.
31616929	5	43	theme	electrophoresis	688:702	arg1	MS					729:730	MS	729:730	MS	729:730	Using capillary zone electrophoresis (CZE)-mass spectrometry (MS) and CZE-MS/MS relying on negative electron transfer dissociation, the major components of healthy human urinary GAGs were determined.
31616929	5	43	theme	electrophoresis	688:702	arg1	spectrometry					715:726	capillary zone electrophoresis (CZE)-mass spectrometry	673:726	capillary zone electrophoresis (CZE)-mass spectrometry (MS)	673:731	Using capillary zone electrophoresis (CZE)-mass spectrometry (MS) and CZE-MS/MS relying on negative electron transfer dissociation, the major components of healthy human urinary GAGs were determined.
31616929	2	44	theme	urinary	312:318	arg1	GAGs					320:323	urinary GAGs	312:323	urinary GAGs	312:323	In order to effectively utilize such information, a detailed understanding of urinary GAGs in healthy individuals can provide insight into the levels and structures of human urinary GAGs.
31616929	5	45	theme	urinary	837:843	arg1	GAGs					845:848	healthy human urinary GAGs	823:848	healthy human urinary GAGs	823:848	Using capillary zone electrophoresis (CZE)-mass spectrometry (MS) and CZE-MS/MS relying on negative electron transfer dissociation, the major components of healthy human urinary GAGs were determined.
31616929	2	46	dep	levels	377:382	arg1	the					373:375	the	373:375	the	373:375	In order to effectively utilize such information, a detailed understanding of urinary GAGs in healthy individuals can provide insight into the levels and structures of human urinary GAGs.
31616929	4	47	theme	molecular	590:598	arg1	distribution					607:618	molecular weight distribution	590:618	molecular weight distribution	590:618	The total creatinine-normalized urinary GAG content, molecular weight distribution and disaccharide compositions were determined.
31616929	3	48	theme	healthy	483:489	arg1	males					491:495	males	491:495	males	491:495	In this study, urinary GAGs were collected and purified from healthy males and females of adults and young adults.
31616929	1	49	theme	various	216:222	arg1	diseases					224:231	various diseases	216:231	various diseases	216:231	Urinary glycosaminoglycans (GAGs) can reflect the health condition of a human being, and the GAGs composition can be directly related to various diseases.
31616929	5	50	theme	transfer	776:783	arg1	dissociation					785:796	negative electron transfer dissociation	758:796	negative electron transfer dissociation	758:796	Using capillary zone electrophoresis (CZE)-mass spectrometry (MS) and CZE-MS/MS relying on negative electron transfer dissociation, the major components of healthy human urinary GAGs were determined.
33901559	0	0	theme	conjugate	71:79	arg1	Influence					0:8	Influence	0:8	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.	0:192	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	2	1	theme	chemical	505:512	arg1	composition					514:524	The chemical composition	501:524	The chemical composition	501:524	The chemical composition was optimized using Taguchi design.
33901559	8	2	theme	E	1390:1390	arg1	eugenol					1398:1404	E:SCMDC eugenol	1390:1404	E:SCMDC eugenol	1390:1404	In comparison to native eugenol, E:SCMDC eugenol showed reduced toxicity.
33901559	5	3	from	dispersion	937:946	arg1	buffer					982:987	buffer	982:987	buffer	982:987	The dispersion of SCMDC eugenol nanoparticles in buffer was prepared to study its stability at different pH (3.0, 5.0, and 7.0) and temperature (4°, 37°, 60 °C) range.
33901559	6	4	dep	%	1150:1150	arg1	74					1148:1149	74	1148:1149	74	1148:1149	In-vitro enzymatic release study showed 31 and 74% release of eugenol after 6 h at pH 2.4 and 7.4, respectively.
33901559	6	4	dep	%	1150:1150	arg1	31					1141:1142	31	1141:1142	31	1141:1142	In-vitro enzymatic release study showed 31 and 74% release of eugenol after 6 h at pH 2.4 and 7.4, respectively.
33901559	0	5	theme	Maillard	62:69	arg1	conjugate					71:79	their Maillard conjugate	56:79	their Maillard conjugate	56:79	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	1	6	theme	sodium	317:322	arg1	conjugate-SCMDC					347:361	sodium caseinate maltodextrin conjugate-SCMDC	317:361	sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC	317:402	The influence of protein (sodium caseinate-SC), polysaccharide (maltodextrin-MD; pectin-PC) and their Maillard conjugates (sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC) were studied on the physico-chemical and biological properties of eugenol nanoemulsions/powder.
33901559	4	7	theme	higher	813:818	arg1	stability					820:828	higher stability	813:828	higher stability of nanoemulsion prepared with Maillard conjugate (SCMDC)	813:885	Turbidity studies revealed higher stability of nanoemulsion prepared with Maillard conjugate (SCMDC) compared to protein or polysaccharides alone.
33901559	1	8	theme	physico-chemical	425:440	arg1	properties					457:466	the physico-chemical and biological properties	421:466	the physico-chemical and biological properties of eugenol nanoemulsions/powder	421:498	The influence of protein (sodium caseinate-SC), polysaccharide (maltodextrin-MD; pectin-PC) and their Maillard conjugates (sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC) were studied on the physico-chemical and biological properties of eugenol nanoemulsions/powder.
33901559	0	9	dep	in	99:100	arg1	vitro					102:106	vitro	102:106	vitro	102:106	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	0	10	from	Influence	0:8	arg1	release					108:114	in vitro release	99:114	in vitro release	99:114	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	0	10	from	Influence	0:8	arg1	property					130:137	anti-oxidant property	117:137	anti-oxidant property	117:137	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	0	10	from	Influence	0:8	arg1	viability					148:156	cell viability	143:156	cell viability	143:156	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	0	10	from	Influence	0:8	arg1	stability					88:96	stability	88:96	stability	88:96	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	0	11	theme	oil	175:177	arg1	nanoemulsions					179:191	eugenol-olive oil nanoemulsions	161:191	eugenol-olive oil nanoemulsions	161:191	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	7	12	theme	antioxidant	1223:1233	arg1	capacity					1235:1242	In vitro antioxidant capacity	1214:1242	In vitro antioxidant capacity of SCMDC encapsulated eugenol	1214:1272	In vitro antioxidant capacity of SCMDC encapsulated eugenol was higher than native eugenol, as demonstrated by free radical scavenging assays.
33901559	7	13	theme	eugenol	1266:1272	arg1	capacity					1235:1242	In vitro antioxidant capacity	1214:1242	In vitro antioxidant capacity of SCMDC encapsulated eugenol	1214:1272	In vitro antioxidant capacity of SCMDC encapsulated eugenol was higher than native eugenol, as demonstrated by free radical scavenging assays.
33901559	2	14	theme	Taguchi	546:552	arg1	design					554:559	Taguchi design	546:559	Taguchi design	546:559	The chemical composition was optimized using Taguchi design.
33901559	1	15	theme	maltodextrin	334:345	arg1	conjugate-SCMDC					347:361	sodium caseinate maltodextrin conjugate-SCMDC	317:361	sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC	317:402	The influence of protein (sodium caseinate-SC), polysaccharide (maltodextrin-MD; pectin-PC) and their Maillard conjugates (sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC) were studied on the physico-chemical and biological properties of eugenol nanoemulsions/powder.
33901559	0	16	theme	eugenol-olive	161:173	arg1	nanoemulsions					179:191	eugenol-olive oil nanoemulsions	161:191	eugenol-olive oil nanoemulsions	161:191	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	1	17	theme	biological	446:455	arg1	properties					457:466	the physico-chemical and biological properties	421:466	the physico-chemical and biological properties of eugenol nanoemulsions/powder	421:498	The influence of protein (sodium caseinate-SC), polysaccharide (maltodextrin-MD; pectin-PC) and their Maillard conjugates (sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC) were studied on the physico-chemical and biological properties of eugenol nanoemulsions/powder.
33901559	0	18	dep	stability	88:96	arg1	the					84:86	the	84:86	the	84:86	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	9	19	from	potential	1508:1516	arg1	applications					1551:1562	nutraceutical and therapeutic applications	1521:1562	nutraceutical and therapeutic applications	1521:1562	These findings suggested that nanoencapsulated eugenol (E:SCMDC) have a huge potential in nutraceutical and therapeutic applications.
33901559	9	20	theme	nanoencapsulated	1461:1476	arg1	E					1487:1487	E	1487:1487	E:SCMDC	1487:1493	These findings suggested that nanoencapsulated eugenol (E:SCMDC) have a huge potential in nutraceutical and therapeutic applications.
33901559	9	20	theme	nanoencapsulated	1461:1476	arg1	eugenol					1478:1484	nanoencapsulated eugenol	1461:1484	nanoencapsulated eugenol (E:SCMDC)	1461:1494	These findings suggested that nanoencapsulated eugenol (E:SCMDC) have a huge potential in nutraceutical and therapeutic applications.
33901559	6	21	dep	release	1152:1158	arg1	%					1150:1150	%	1150:1150	%	1150:1150	In-vitro enzymatic release study showed 31 and 74% release of eugenol after 6 h at pH 2.4 and 7.4, respectively.
33901559	4	22	theme	Turbidity	786:794	arg1	studies					796:802	Turbidity studies	786:802	Turbidity studies	786:802	Turbidity studies revealed higher stability of nanoemulsion prepared with Maillard conjugate (SCMDC) compared to protein or polysaccharides alone.
33901559	3	23	dep	SC	611:612	arg1	nm					681:682	104.6, 323.5, 1872, 181.7, and 454.4 nm	644:682	104.6, 323.5, 1872, 181.7, and 454.4 nm	644:682	The particles size of eugenol nanoemulsions with SC, MD, PC, SCMDC and SCPCC were 104.6, 323.5, 1872, 181.7, and 454.4 nm, respectively while their zeta potentials were -31.2, -28.5, -21.4, -40.1 and -25.1 mV, respectively.
33901559	0	24	theme	nanoemulsions	179:191	arg1	release					108:114	in vitro release	99:114	in vitro release	99:114	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	0	24	theme	nanoemulsions	179:191	arg1	property					130:137	anti-oxidant property	117:137	anti-oxidant property	117:137	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	0	24	theme	nanoemulsions	179:191	arg1	viability					148:156	cell viability	143:156	cell viability	143:156	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	0	24	theme	nanoemulsions	179:191	arg1	stability					88:96	stability	88:96	stability	88:96	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	6	25	from	7.4	1195:1197	arg1	h					1179:1179	6 h	1177:1179	6 h at pH 2.4 and 7.4	1177:1197	In-vitro enzymatic release study showed 31 and 74% release of eugenol after 6 h at pH 2.4 and 7.4, respectively.
33901559	7	26	theme	native	1290:1295	arg1	eugenol					1297:1303	native eugenol	1290:1303	native eugenol	1290:1303	In vitro antioxidant capacity of SCMDC encapsulated eugenol was higher than native eugenol, as demonstrated by free radical scavenging assays.
33901559	6	27	from	pH	1184:1185	arg1	h					1179:1179	6 h	1177:1179	6 h at pH 2.4 and 7.4	1177:1197	In-vitro enzymatic release study showed 31 and 74% release of eugenol after 6 h at pH 2.4 and 7.4, respectively.
33901559	1	28	theme	sodium	364:369	arg1	conjugate-SCPCC					388:402	sodium caseinate pectin conjugate-SCPCC	364:402	sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC	317:402	The influence of protein (sodium caseinate-SC), polysaccharide (maltodextrin-MD; pectin-PC) and their Maillard conjugates (sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC) were studied on the physico-chemical and biological properties of eugenol nanoemulsions/powder.
33901559	9	29	theme	nutraceutical	1521:1533	arg1	applications					1551:1562	nutraceutical and therapeutic applications	1521:1562	nutraceutical and therapeutic applications	1521:1562	These findings suggested that nanoencapsulated eugenol (E:SCMDC) have a huge potential in nutraceutical and therapeutic applications.
33901559	1	30	theme	eugenol	471:477	arg1	nanoemulsions/powder					479:498	eugenol nanoemulsions/powder	471:498	eugenol nanoemulsions/powder	471:498	The influence of protein (sodium caseinate-SC), polysaccharide (maltodextrin-MD; pectin-PC) and their Maillard conjugates (sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC) were studied on the physico-chemical and biological properties of eugenol nanoemulsions/powder.
33901559	3	31	theme	nanoemulsions	592:604	arg1	size					576:579	size	576:579	size of eugenol nanoemulsions with SC, MD, PC, SCMDC and SCPCC were 104.6, 323.5, 1872, 181.7, and 454.4 nm, respectively while their zeta potentials were -31.2, -28.5, -21.4, -40.1 and -25.1 mV, respectively	576:783	The particles size of eugenol nanoemulsions with SC, MD, PC, SCMDC and SCPCC were 104.6, 323.5, 1872, 181.7, and 454.4 nm, respectively while their zeta potentials were -31.2, -28.5, -21.4, -40.1 and -25.1 mV, respectively.
33901559	7	32	theme	free	1325:1328	arg1	scavenging					1338:1347	free radical scavenging	1325:1347	free radical scavenging assays	1325:1354	In vitro antioxidant capacity of SCMDC encapsulated eugenol was higher than native eugenol, as demonstrated by free radical scavenging assays.
33901559	9	33	contain	have	1496:1499	arg1	E					1487:1487	E	1487:1487	E:SCMDC	1487:1493	These findings suggested that nanoencapsulated eugenol (E:SCMDC) have a huge potential in nutraceutical and therapeutic applications.
33901559	9	33	contain	have	1496:1499	arg1	eugenol					1478:1484	nanoencapsulated eugenol	1461:1484	nanoencapsulated eugenol (E:SCMDC)	1461:1494	These findings suggested that nanoencapsulated eugenol (E:SCMDC) have a huge potential in nutraceutical and therapeutic applications.
33901559	9	33	contain	have	1496:1499	arg2	potential					1508:1516	a huge potential	1501:1516	a huge potential in nutraceutical and therapeutic applications	1501:1562	These findings suggested that nanoencapsulated eugenol (E:SCMDC) have a huge potential in nutraceutical and therapeutic applications.
33901559	7	34	theme	radical	1330:1336	arg1	scavenging					1338:1347	free radical scavenging	1325:1347	free radical scavenging assays	1325:1354	In vitro antioxidant capacity of SCMDC encapsulated eugenol was higher than native eugenol, as demonstrated by free radical scavenging assays.
33901559	1	35	theme	nanoemulsions/powder	479:498	arg1	properties					457:466	the physico-chemical and biological properties	421:466	the physico-chemical and biological properties of eugenol nanoemulsions/powder	421:498	The influence of protein (sodium caseinate-SC), polysaccharide (maltodextrin-MD; pectin-PC) and their Maillard conjugates (sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC) were studied on the physico-chemical and biological properties of eugenol nanoemulsions/powder.
33901559	7	36	theme	In	1214:1215	arg1	capacity					1235:1242	In vitro antioxidant capacity	1214:1242	In vitro antioxidant capacity of SCMDC encapsulated eugenol	1214:1272	In vitro antioxidant capacity of SCMDC encapsulated eugenol was higher than native eugenol, as demonstrated by free radical scavenging assays.
33901559	5	37	theme	different	1028:1036	arg1	pH					1038:1039	different pH	1028:1039	different pH (3.0, 5.0, and 7.0)	1028:1059	The dispersion of SCMDC eugenol nanoparticles in buffer was prepared to study its stability at different pH (3.0, 5.0, and 7.0) and temperature (4°, 37°, 60 °C) range.
33901559	5	37	theme	different	1028:1036	arg1	°C					1090:1091	4°, 37°, 60 °C	1078:1091	4°, 37°, 60 °C	1078:1091	The dispersion of SCMDC eugenol nanoparticles in buffer was prepared to study its stability at different pH (3.0, 5.0, and 7.0) and temperature (4°, 37°, 60 °C) range.
33901559	0	38	theme	caseinate	20:28	arg1	Influence					0:8	Influence	0:8	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.	0:192	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	8	39	theme	SCMDC	1392:1396	arg1	eugenol					1398:1404	E:SCMDC eugenol	1390:1404	E:SCMDC eugenol	1390:1404	In comparison to native eugenol, E:SCMDC eugenol showed reduced toxicity.
33901559	9	40	theme	therapeutic	1539:1549	arg1	applications					1551:1562	nutraceutical and therapeutic applications	1521:1562	nutraceutical and therapeutic applications	1521:1562	These findings suggested that nanoencapsulated eugenol (E:SCMDC) have a huge potential in nutraceutical and therapeutic applications.
33901559	6	41	theme	eugenol	1163:1169	arg1	release					1152:1158	31 and 74% release	1141:1158	31 and 74% release of eugenol	1141:1169	In-vitro enzymatic release study showed 31 and 74% release of eugenol after 6 h at pH 2.4 and 7.4, respectively.
33901559	7	42	theme	encapsulated	1253:1264	arg1	eugenol					1266:1272	SCMDC encapsulated eugenol	1247:1272	SCMDC encapsulated eugenol	1247:1272	In vitro antioxidant capacity of SCMDC encapsulated eugenol was higher than native eugenol, as demonstrated by free radical scavenging assays.
33901559	0	43	theme	sodium	13:18	arg1	caseinate					20:28	sodium caseinate	13:28	sodium caseinate	13:28	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	8	44	theme	reduced	1413:1419	arg1	toxicity					1421:1428	reduced toxicity	1413:1428	reduced toxicity	1413:1428	In comparison to native eugenol, E:SCMDC eugenol showed reduced toxicity.
33901559	0	45	theme	in	99:100	arg1	release					108:114	in vitro release	99:114	in vitro release	99:114	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	1	46	theme	caseinate	371:379	arg1	conjugate-SCPCC					388:402	sodium caseinate pectin conjugate-SCPCC	364:402	sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC	317:402	The influence of protein (sodium caseinate-SC), polysaccharide (maltodextrin-MD; pectin-PC) and their Maillard conjugates (sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC) were studied on the physico-chemical and biological properties of eugenol nanoemulsions/powder.
33901559	3	47	theme	eugenol	584:590	arg1	nanoemulsions					592:604	eugenol nanoemulsions	584:604	eugenol nanoemulsions with SC, MD, PC, SCMDC and SCPCC were 104.6, 323.5, 1872, 181.7, and 454.4 nm, respectively while their zeta potentials were -31.2, -28.5, -21.4, -40.1 and -25.1 mV, respectively	584:783	The particles size of eugenol nanoemulsions with SC, MD, PC, SCMDC and SCPCC were 104.6, 323.5, 1872, 181.7, and 454.4 nm, respectively while their zeta potentials were -31.2, -28.5, -21.4, -40.1 and -25.1 mV, respectively.
33901559	0	48	theme	maltodextrin	31:42	arg1	Influence					0:8	Influence	0:8	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.	0:192	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	3	49	theme	zeta	710:713	arg1	potentials					715:724	their zeta potentials	704:724	their zeta potentials	704:724	The particles size of eugenol nanoemulsions with SC, MD, PC, SCMDC and SCPCC were 104.6, 323.5, 1872, 181.7, and 454.4 nm, respectively while their zeta potentials were -31.2, -28.5, -21.4, -40.1 and -25.1 mV, respectively.
33901559	1	50	theme	pectin	381:386	arg1	conjugate-SCPCC					388:402	sodium caseinate pectin conjugate-SCPCC	364:402	sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC	317:402	The influence of protein (sodium caseinate-SC), polysaccharide (maltodextrin-MD; pectin-PC) and their Maillard conjugates (sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC) were studied on the physico-chemical and biological properties of eugenol nanoemulsions/powder.
33901559	7	51	dep	In	1214:1215	arg1	vitro					1217:1221	vitro	1217:1221	vitro	1217:1221	In vitro antioxidant capacity of SCMDC encapsulated eugenol was higher than native eugenol, as demonstrated by free radical scavenging assays.
33901559	5	52	theme	temperature	1065:1075	arg1	range					1094:1098	different pH (3.0, 5.0, and 7.0) and temperature (4°, 37°, 60 °C) range	1028:1098	different pH (3.0, 5.0, and 7.0) and temperature (4°, 37°, 60 °C) range	1028:1098	The dispersion of SCMDC eugenol nanoparticles in buffer was prepared to study its stability at different pH (3.0, 5.0, and 7.0) and temperature (4°, 37°, 60 °C) range.
33901559	5	53	from	range	1094:1098	arg1	stability					1015:1023	its stability	1011:1023	its stability at different pH (3.0, 5.0, and 7.0) and temperature (4°, 37°, 60 °C) range	1011:1098	The dispersion of SCMDC eugenol nanoparticles in buffer was prepared to study its stability at different pH (3.0, 5.0, and 7.0) and temperature (4°, 37°, 60 °C) range.
33901559	5	54	theme	pH	1038:1039	arg1	range					1094:1098	different pH (3.0, 5.0, and 7.0) and temperature (4°, 37°, 60 °C) range	1028:1098	different pH (3.0, 5.0, and 7.0) and temperature (4°, 37°, 60 °C) range	1028:1098	The dispersion of SCMDC eugenol nanoparticles in buffer was prepared to study its stability at different pH (3.0, 5.0, and 7.0) and temperature (4°, 37°, 60 °C) range.
33901559	3	55	with	nanoemulsions	592:604	arg1	MD					615:616	SC, MD, PC, SCMDC and SCPCC were 104.6, 323.5, 1872, 181.7, and 454.4 nm, respectively while their zeta potentials were -31.2, -28.5, -21.4, -40.1 and -25.1 mV, respectively	611:783	MD	615:616	The particles size of eugenol nanoemulsions with SC, MD, PC, SCMDC and SCPCC were 104.6, 323.5, 1872, 181.7, and 454.4 nm, respectively while their zeta potentials were -31.2, -28.5, -21.4, -40.1 and -25.1 mV, respectively.
33901559	3	55	with	nanoemulsions	592:604	arg1	SCPCC					633:637	SC, MD, PC, SCMDC and SCPCC were 104.6, 323.5, 1872, 181.7, and 454.4 nm, respectively while their zeta potentials were -31.2, -28.5, -21.4, -40.1 and -25.1 mV, respectively	611:783	SCPCC	633:637	The particles size of eugenol nanoemulsions with SC, MD, PC, SCMDC and SCPCC were 104.6, 323.5, 1872, 181.7, and 454.4 nm, respectively while their zeta potentials were -31.2, -28.5, -21.4, -40.1 and -25.1 mV, respectively.
33901559	3	55	with	nanoemulsions	592:604	arg1	SCMDC					623:627	SC, MD, PC, SCMDC and SCPCC were 104.6, 323.5, 1872, 181.7, and 454.4 nm, respectively while their zeta potentials were -31.2, -28.5, -21.4, -40.1 and -25.1 mV, respectively	611:783	SCMDC	623:627	The particles size of eugenol nanoemulsions with SC, MD, PC, SCMDC and SCPCC were 104.6, 323.5, 1872, 181.7, and 454.4 nm, respectively while their zeta potentials were -31.2, -28.5, -21.4, -40.1 and -25.1 mV, respectively.
33901559	3	55	with	nanoemulsions	592:604	arg1	SC					611:612	SC, MD, PC, SCMDC and SCPCC were 104.6, 323.5, 1872, 181.7, and 454.4 nm, respectively while their zeta potentials were -31.2, -28.5, -21.4, -40.1 and -25.1 mV, respectively	611:783	SC	611:612	The particles size of eugenol nanoemulsions with SC, MD, PC, SCMDC and SCPCC were 104.6, 323.5, 1872, 181.7, and 454.4 nm, respectively while their zeta potentials were -31.2, -28.5, -21.4, -40.1 and -25.1 mV, respectively.
33901559	3	55	with	nanoemulsions	592:604	arg1	PC					619:620	SC, MD, PC, SCMDC and SCPCC were 104.6, 323.5, 1872, 181.7, and 454.4 nm, respectively while their zeta potentials were -31.2, -28.5, -21.4, -40.1 and -25.1 mV, respectively	611:783	PC	619:620	The particles size of eugenol nanoemulsions with SC, MD, PC, SCMDC and SCPCC were 104.6, 323.5, 1872, 181.7, and 454.4 nm, respectively while their zeta potentials were -31.2, -28.5, -21.4, -40.1 and -25.1 mV, respectively.
33901559	1	56	dep	conjugate-SCMDC	347:361	arg1	conjugate-SCPCC					388:402	sodium caseinate pectin conjugate-SCPCC	364:402	sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC	317:402	The influence of protein (sodium caseinate-SC), polysaccharide (maltodextrin-MD; pectin-PC) and their Maillard conjugates (sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC) were studied on the physico-chemical and biological properties of eugenol nanoemulsions/powder.
33901559	0	57	theme	pectin	45:50	arg1	Influence					0:8	Influence	0:8	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.	0:192	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	6	58	theme	release	1120:1126	arg1	study					1128:1132	In-vitro enzymatic release study	1101:1132	In-vitro enzymatic release study	1101:1132	In-vitro enzymatic release study showed 31 and 74% release of eugenol after 6 h at pH 2.4 and 7.4, respectively.
33901559	1	59	theme	caseinate	324:332	arg1	conjugate-SCMDC					347:361	sodium caseinate maltodextrin conjugate-SCMDC	317:361	sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC	317:402	The influence of protein (sodium caseinate-SC), polysaccharide (maltodextrin-MD; pectin-PC) and their Maillard conjugates (sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC) were studied on the physico-chemical and biological properties of eugenol nanoemulsions/powder.
33901559	1	60	dep	maltodextrin-MD	258:272	arg1	pectin-PC					275:283	pectin-PC	275:283	maltodextrin-MD; pectin-PC	258:283	The influence of protein (sodium caseinate-SC), polysaccharide (maltodextrin-MD; pectin-PC) and their Maillard conjugates (sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC) were studied on the physico-chemical and biological properties of eugenol nanoemulsions/powder.
33901559	0	61	theme	anti-oxidant	117:128	arg1	property					130:137	anti-oxidant property	117:137	anti-oxidant property	117:137	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	6	62	theme	enzymatic	1110:1118	arg1	study					1128:1132	In-vitro enzymatic release study	1101:1132	In-vitro enzymatic release study	1101:1132	In-vitro enzymatic release study showed 31 and 74% release of eugenol after 6 h at pH 2.4 and 7.4, respectively.
33901559	9	63	theme	huge	1503:1506	arg1	potential					1508:1516	a huge potential	1501:1516	a huge potential in nutraceutical and therapeutic applications	1501:1562	These findings suggested that nanoencapsulated eugenol (E:SCMDC) have a huge potential in nutraceutical and therapeutic applications.
33901559	4	64	theme	nanoemulsion	833:844	arg1	stability					820:828	higher stability	813:828	higher stability of nanoemulsion prepared with Maillard conjugate (SCMDC)	813:885	Turbidity studies revealed higher stability of nanoemulsion prepared with Maillard conjugate (SCMDC) compared to protein or polysaccharides alone.
33901559	1	65	theme	Maillard	296:303	arg1	conjugates					305:314	their Maillard conjugates	290:314	their Maillard conjugates	290:314	The influence of protein (sodium caseinate-SC), polysaccharide (maltodextrin-MD; pectin-PC) and their Maillard conjugates (sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC) were studied on the physico-chemical and biological properties of eugenol nanoemulsions/powder.
33901559	1	65	theme	Maillard	296:303	arg1	protein					211:217	protein	211:217	protein (sodium caseinate-SC)	211:239	The influence of protein (sodium caseinate-SC), polysaccharide (maltodextrin-MD; pectin-PC) and their Maillard conjugates (sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC) were studied on the physico-chemical and biological properties of eugenol nanoemulsions/powder.
33901559	0	66	theme	cell	143:146	arg1	viability					148:156	cell viability	143:156	cell viability	143:156	Influence of sodium caseinate, maltodextrin, pectin and their Maillard conjugate on the stability, in vitro release, anti-oxidant property and cell viability of eugenol-olive oil nanoemulsions.
33901559	1	67	dep	polysaccharide	242:255	arg1	conjugate-SCMDC					347:361	sodium caseinate maltodextrin conjugate-SCMDC	317:361	sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC	317:402	The influence of protein (sodium caseinate-SC), polysaccharide (maltodextrin-MD; pectin-PC) and their Maillard conjugates (sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC) were studied on the physico-chemical and biological properties of eugenol nanoemulsions/powder.
33901559	1	67	dep	polysaccharide	242:255	arg1	maltodextrin-MD					258:272	maltodextrin-MD	258:272	maltodextrin-MD; pectin-PC	258:283	The influence of protein (sodium caseinate-SC), polysaccharide (maltodextrin-MD; pectin-PC) and their Maillard conjugates (sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC) were studied on the physico-chemical and biological properties of eugenol nanoemulsions/powder.
33901559	6	68	theme	In-vitro	1101:1108	arg1	study					1128:1132	In-vitro enzymatic release study	1101:1132	In-vitro enzymatic release study	1101:1132	In-vitro enzymatic release study showed 31 and 74% release of eugenol after 6 h at pH 2.4 and 7.4, respectively.
33901559	7	69	theme	SCMDC	1247:1251	arg1	eugenol					1266:1272	SCMDC encapsulated eugenol	1247:1272	SCMDC encapsulated eugenol	1247:1272	In vitro antioxidant capacity of SCMDC encapsulated eugenol was higher than native eugenol, as demonstrated by free radical scavenging assays.
33901559	7	70	theme	scavenging	1338:1347	arg1	assays					1349:1354	free radical scavenging assays	1325:1354	free radical scavenging assays	1325:1354	In vitro antioxidant capacity of SCMDC encapsulated eugenol was higher than native eugenol, as demonstrated by free radical scavenging assays.
33901559	5	71	dep	pH	1038:1039	arg1	5.0					1047:1049	5.0	1047:1049	5.0	1047:1049	The dispersion of SCMDC eugenol nanoparticles in buffer was prepared to study its stability at different pH (3.0, 5.0, and 7.0) and temperature (4°, 37°, 60 °C) range.
33901559	5	71	dep	pH	1038:1039	arg1	7.0					1056:1058	7.0	1056:1058	7.0	1056:1058	The dispersion of SCMDC eugenol nanoparticles in buffer was prepared to study its stability at different pH (3.0, 5.0, and 7.0) and temperature (4°, 37°, 60 °C) range.
33901559	4	72	theme	Maillard	860:867	arg1	SCMDC					880:884	SCMDC	880:884	SCMDC	880:884	Turbidity studies revealed higher stability of nanoemulsion prepared with Maillard conjugate (SCMDC) compared to protein or polysaccharides alone.
33901559	4	72	theme	Maillard	860:867	arg1	conjugate					869:877	Maillard conjugate	860:877	Maillard conjugate (SCMDC)	860:885	Turbidity studies revealed higher stability of nanoemulsion prepared with Maillard conjugate (SCMDC) compared to protein or polysaccharides alone.
33901559	5	73	theme	SCMDC	951:955	arg1	nanoparticles					965:977	SCMDC eugenol nanoparticles	951:977	SCMDC eugenol nanoparticles	951:977	The dispersion of SCMDC eugenol nanoparticles in buffer was prepared to study its stability at different pH (3.0, 5.0, and 7.0) and temperature (4°, 37°, 60 °C) range.
33901559	3	74	dep	size	576:579	arg1	particles					566:574	The particles	562:574	The particles	562:574	The particles size of eugenol nanoemulsions with SC, MD, PC, SCMDC and SCPCC were 104.6, 323.5, 1872, 181.7, and 454.4 nm, respectively while their zeta potentials were -31.2, -28.5, -21.4, -40.1 and -25.1 mV, respectively.
33901559	1	75	theme	protein	211:217	arg1	influence					198:206	The influence	194:206	The influence of protein (sodium caseinate-SC), polysaccharide (maltodextrin-MD; pectin-PC) and their Maillard conjugates (sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC)	194:403	The influence of protein (sodium caseinate-SC), polysaccharide (maltodextrin-MD; pectin-PC) and their Maillard conjugates (sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC) were studied on the physico-chemical and biological properties of eugenol nanoemulsions/powder.
33901559	8	76	theme	native	1374:1379	arg1	eugenol					1381:1387	native eugenol	1374:1387	native eugenol	1374:1387	In comparison to native eugenol, E:SCMDC eugenol showed reduced toxicity.
33901559	9	77	dep	E	1487:1487	arg1	SCMDC					1489:1493	SCMDC	1489:1493	E:SCMDC	1487:1493	These findings suggested that nanoencapsulated eugenol (E:SCMDC) have a huge potential in nutraceutical and therapeutic applications.
33901559	5	78	theme	eugenol	957:963	arg1	nanoparticles					965:977	SCMDC eugenol nanoparticles	951:977	SCMDC eugenol nanoparticles	951:977	The dispersion of SCMDC eugenol nanoparticles in buffer was prepared to study its stability at different pH (3.0, 5.0, and 7.0) and temperature (4°, 37°, 60 °C) range.
33901559	5	79	theme	nanoparticles	965:977	arg1	dispersion					937:946	The dispersion	933:946	The dispersion of SCMDC eugenol nanoparticles in buffer	933:987	The dispersion of SCMDC eugenol nanoparticles in buffer was prepared to study its stability at different pH (3.0, 5.0, and 7.0) and temperature (4°, 37°, 60 °C) range.
33901559	1	80	theme	sodium	220:225	arg1	protein					211:217	protein	211:217	protein (sodium caseinate-SC)	211:239	The influence of protein (sodium caseinate-SC), polysaccharide (maltodextrin-MD; pectin-PC) and their Maillard conjugates (sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC) were studied on the physico-chemical and biological properties of eugenol nanoemulsions/powder.
33901559	1	80	theme	sodium	220:225	arg1	caseinate-SC					227:238	sodium caseinate-SC	220:238	sodium caseinate-SC	220:238	The influence of protein (sodium caseinate-SC), polysaccharide (maltodextrin-MD; pectin-PC) and their Maillard conjugates (sodium caseinate maltodextrin conjugate-SCMDC; sodium caseinate pectin conjugate-SCPCC) were studied on the physico-chemical and biological properties of eugenol nanoemulsions/powder.
32302634	8	0	contain	have	959:962	arg1	Gels					935:938	Gels	935:938	Gels of red rice starch	935:957	Gels of red rice starch have higher syneresis in five freeze-thaw cycles, when compared to the others.
32302634	8	0	contain	have	959:962	arg2	syneresis					971:979	higher syneresis	964:979	higher syneresis	964:979	Gels of red rice starch have higher syneresis in five freeze-thaw cycles, when compared to the others.
32302634	6	1	theme	rice	799:802	arg1	starches					766:773	the starches	762:773	the starches of white, red and black rice	762:802	With regard to pasting properties of the starches, differences were observed in initial temperature, which was of 80.6 °C, 79.1 °C and 88.8 °C for the starches of white, red and black rice, respectively.
32302634	8	2	theme	starch	952:957	arg1	Gels					935:938	Gels	935:938	Gels of red rice starch	935:957	Gels of red rice starch have higher syneresis in five freeze-thaw cycles, when compared to the others.
32302634	1	3	from	varieties	136:144	arg1	starch					118:123	starch	118:123	starch	118:123	The aim of this work was to extract and characterize starch from three varieties of pigmented rice (named white, red and black), preserving the bioactive compounds.
32302634	1	4	theme	bioactive	209:217	arg1	compounds					219:227	the bioactive compounds	205:227	the bioactive compounds	205:227	The aim of this work was to extract and characterize starch from three varieties of pigmented rice (named white, red and black), preserving the bioactive compounds.
32302634	4	5	theme	chemical	444:451	arg1	analyzes					453:460	The chemical analyzes	440:460	The chemical analyzes	440:460	The chemical analyzes, showed more than 83.0% of carbohydrates in the three compositions.
32302634	5	6	theme	raw	585:587	arg1	material					589:596	the raw material	581:596	the raw material	581:596	There was retention of the phenolic compounds from the raw material in the starches.
32302634	7	7	theme	higher	858:863	arg1	crystallinity					865:877	slightly higher crystallinity	849:877	slightly higher crystallinity	849:877	Black rice starch also showed slightly higher crystallinity and thermal stability than white and red rice starches.
32302634	7	8	theme	thermal	883:889	arg1	stability					891:899	thermal stability	883:899	thermal stability	883:899	Black rice starch also showed slightly higher crystallinity and thermal stability than white and red rice starches.
32302634	0	9	from	rice	59:62	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Extraction and characterization of starches from pigmented rice.
32302634	0	9	from	rice	59:62	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and characterization of starches from pigmented rice.
32302634	6	10	theme	pasting	630:636	arg1	properties					638:647	pasting properties	630:647	pasting properties of the starches	630:663	With regard to pasting properties of the starches, differences were observed in initial temperature, which was of 80.6 °C, 79.1 °C and 88.8 °C for the starches of white, red and black rice, respectively.
32302634	6	11	theme	red	785:787	arg1	rice					799:802	white, red and black rice	778:802	white, red and black rice	778:802	With regard to pasting properties of the starches, differences were observed in initial temperature, which was of 80.6 °C, 79.1 °C and 88.8 °C for the starches of white, red and black rice, respectively.
32302634	8	12	theme	red	943:945	arg1	starch					952:957	red rice starch	943:957	red rice starch	943:957	Gels of red rice starch have higher syneresis in five freeze-thaw cycles, when compared to the others.
32302634	2	13	theme	extraction	234:243	arg1	yield					245:249	The extraction yield	230:249	The extraction yield	230:249	The extraction yield was 44.0%, 47.0% and 35.7%, respectively.
32302634	2	13	theme	extraction	234:243	arg1	%					259:259	44.0%	255:259	44.0%	255:259	The extraction yield was 44.0%, 47.0% and 35.7%, respectively.
32302634	1	14	theme	pigmented	149:157	arg1	rice					159:162	pigmented rice	149:162	pigmented rice (named white, red and black)	149:191	The aim of this work was to extract and characterize starch from three varieties of pigmented rice (named white, red and black), preserving the bioactive compounds.
32302634	7	15	theme	rice	825:828	arg1	starch					830:835	Black rice starch	819:835	Black rice starch	819:835	Black rice starch also showed slightly higher crystallinity and thermal stability than white and red rice starches.
32302634	1	16	theme	rice	159:162	arg1	varieties					136:144	three varieties	130:144	three varieties of pigmented rice (named white, red and black)	130:191	The aim of this work was to extract and characterize starch from three varieties of pigmented rice (named white, red and black), preserving the bioactive compounds.
32302634	1	16	theme	rice	159:162	arg1	rice					159:162	pigmented rice	149:162	pigmented rice (named white, red and black)	149:191	The aim of this work was to extract and characterize starch from three varieties of pigmented rice (named white, red and black), preserving the bioactive compounds.
32302634	6	17	theme	white	778:782	arg1	rice					799:802	white, red and black rice	778:802	white, red and black rice	778:802	With regard to pasting properties of the starches, differences were observed in initial temperature, which was of 80.6 °C, 79.1 °C and 88.8 °C for the starches of white, red and black rice, respectively.
32302634	5	18	theme	phenolic	557:564	arg1	compounds					566:574	the phenolic compounds	553:574	the phenolic compounds	553:574	There was retention of the phenolic compounds from the raw material in the starches.
32302634	5	19	theme	compounds	566:574	arg1	retention					540:548	retention	540:548	retention of the phenolic compounds from the raw material in the starches	540:612	There was retention of the phenolic compounds from the raw material in the starches.
32302634	6	20	theme	initial	695:701	arg1	temperature					703:713	initial temperature	695:713	initial temperature	695:713	With regard to pasting properties of the starches, differences were observed in initial temperature, which was of 80.6 °C, 79.1 °C and 88.8 °C for the starches of white, red and black rice, respectively.
32302634	1	21	theme	work	81:84	arg1	aim					69:71	The aim	65:71	The aim of this work	65:84	The aim of this work was to extract and characterize starch from three varieties of pigmented rice (named white, red and black), preserving the bioactive compounds.
32302634	1	21	theme	work	81:84	arg1	extract					93:99	extract	93:99	extract	93:99	The aim of this work was to extract and characterize starch from three varieties of pigmented rice (named white, red and black), preserving the bioactive compounds.
32302634	3	22	theme	scanning	297:304	arg1	microscopy					315:324	The scanning electron microscopy	293:324	The scanning electron microscopy	293:324	The scanning electron microscopy showed that the granules of the three varieties presented polygonal and angular format and absence of impurities.
32302634	8	23	theme	higher	964:969	arg1	syneresis					971:979	higher syneresis	964:979	higher syneresis	964:979	Gels of red rice starch have higher syneresis in five freeze-thaw cycles, when compared to the others.
32302634	7	24	theme	Black	819:823	arg1	starch					830:835	Black rice starch	819:835	Black rice starch	819:835	Black rice starch also showed slightly higher crystallinity and thermal stability than white and red rice starches.
32302634	3	25	theme	angular	398:404	arg1	format					406:411	polygonal and angular format	384:411	polygonal and angular format	384:411	The scanning electron microscopy showed that the granules of the three varieties presented polygonal and angular format and absence of impurities.
32302634	3	26	theme	electron	306:313	arg1	microscopy					315:324	The scanning electron microscopy	293:324	The scanning electron microscopy	293:324	The scanning electron microscopy showed that the granules of the three varieties presented polygonal and angular format and absence of impurities.
32302634	4	27	theme	carbohydrates	489:501	arg1	%					484:484	more than 83.0%	470:484	more than 83.0% of carbohydrates	470:501	The chemical analyzes, showed more than 83.0% of carbohydrates in the three compositions.
32302634	4	27	theme	carbohydrates	489:501	arg1	carbohydrates					489:501	carbohydrates	489:501	carbohydrates	489:501	The chemical analyzes, showed more than 83.0% of carbohydrates in the three compositions.
32302634	3	28	theme	polygonal	384:392	arg1	format					406:411	polygonal and angular format	384:411	polygonal and angular format	384:411	The scanning electron microscopy showed that the granules of the three varieties presented polygonal and angular format and absence of impurities.
32302634	0	29	theme	starches	35:42	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Extraction and characterization of starches from pigmented rice.
32302634	0	29	theme	starches	35:42	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and characterization of starches from pigmented rice.
32302634	6	30	located	observed	683:690	arg1	temperature					703:713	initial temperature	695:713	initial temperature	695:713	With regard to pasting properties of the starches, differences were observed in initial temperature, which was of 80.6 °C, 79.1 °C and 88.8 °C for the starches of white, red and black rice, respectively.
32302634	6	30	located	observed	683:690	arg2	differences					666:676	differences	666:676	differences	666:676	With regard to pasting properties of the starches, differences were observed in initial temperature, which was of 80.6 °C, 79.1 °C and 88.8 °C for the starches of white, red and black rice, respectively.
32302634	5	31	from	material	589:596	arg1	retention					540:548	retention	540:548	retention of the phenolic compounds from the raw material in the starches	540:612	There was retention of the phenolic compounds from the raw material in the starches.
32302634	8	32	theme	freeze-thaw	989:999	arg1	cycles					1001:1006	five freeze-thaw cycles	984:1006	five freeze-thaw cycles	984:1006	Gels of red rice starch have higher syneresis in five freeze-thaw cycles, when compared to the others.
32302634	3	33	theme	varieties	364:372	arg1	granules					342:349	the granules	338:349	the granules of the three varieties	338:372	The scanning electron microscopy showed that the granules of the three varieties presented polygonal and angular format and absence of impurities.
32302634	7	34	theme	red	916:918	arg1	starches					925:932	white and red rice starches	906:932	white and red rice starches	906:932	Black rice starch also showed slightly higher crystallinity and thermal stability than white and red rice starches.
32302634	0	35	theme	pigmented	49:57	arg1	rice					59:62	pigmented rice	49:62	pigmented rice	49:62	Extraction and characterization of starches from pigmented rice.
32302634	1	36	dep	rice	159:162	arg1	named					165:169	named	165:169	named white, red and black	165:190	The aim of this work was to extract and characterize starch from three varieties of pigmented rice (named white, red and black), preserving the bioactive compounds.
32302634	3	37	theme	impurities	428:437	arg1	absence					417:423	absence	417:423	absence of impurities	417:437	The scanning electron microscopy showed that the granules of the three varieties presented polygonal and angular format and absence of impurities.
32302634	3	37	theme	impurities	428:437	arg1	format					406:411	polygonal and angular format	384:411	polygonal and angular format	384:411	The scanning electron microscopy showed that the granules of the three varieties presented polygonal and angular format and absence of impurities.
32302634	7	38	theme	rice	920:923	arg1	starches					925:932	white and red rice starches	906:932	white and red rice starches	906:932	Black rice starch also showed slightly higher crystallinity and thermal stability than white and red rice starches.
32302634	7	39	theme	white	906:910	arg1	starches					925:932	white and red rice starches	906:932	white and red rice starches	906:932	Black rice starch also showed slightly higher crystallinity and thermal stability than white and red rice starches.
32302634	6	40	dep	properties	638:647	arg1	regard					620:625	regard	620:625	regard	620:625	With regard to pasting properties of the starches, differences were observed in initial temperature, which was of 80.6 °C, 79.1 °C and 88.8 °C for the starches of white, red and black rice, respectively.
32302634	6	41	theme	starches	656:663	arg1	properties					638:647	pasting properties	630:647	pasting properties of the starches	630:663	With regard to pasting properties of the starches, differences were observed in initial temperature, which was of 80.6 °C, 79.1 °C and 88.8 °C for the starches of white, red and black rice, respectively.
32302634	8	42	theme	rice	947:950	arg1	starch					952:957	red rice starch	943:957	red rice starch	943:957	Gels of red rice starch have higher syneresis in five freeze-thaw cycles, when compared to the others.
32302634	6	43	theme	black	793:797	arg1	rice					799:802	white, red and black rice	778:802	white, red and black rice	778:802	With regard to pasting properties of the starches, differences were observed in initial temperature, which was of 80.6 °C, 79.1 °C and 88.8 °C for the starches of white, red and black rice, respectively.
32302634	5	44	from	retention	540:548	arg1	starches					605:612	the starches	601:612	the starches	601:612	There was retention of the phenolic compounds from the raw material in the starches.
33535598	4	0	theme	%	468:468	arg1	%					479:479	1.0 wt% to 8.0 wt%	462:479	1.0 wt% to 8.0 wt%	462:479	We tested cellulose paper enriched by TiO2 from 1.0 wt% to 8.0 wt%, respectively.
33535598	1	1	theme	cellulose	146:154	arg1	fibers					156:161	cellulose fibers	146:161	cellulose fibers	146:161	Here, we aimed to boost antibacterial performance of cellulose fibers for paper sheet application.
33535598	7	2	theme	industrial	850:859	arg1	applications					861:872	industrial applications	850:872	industrial applications	850:872	The simplicity, low cost, and reproducibility of the prepared method indicates the potential to be scaled up for industrial applications.
33535598	0	3	theme	TiO2	73:76	arg1	Nanoparticles					78:90	TiO2 Nanoparticles	73:90	TiO2 Nanoparticles	73:90	Boosting of Antibacterial Performance of Cellulose Based Paper Sheet via TiO2 Nanoparticles.
33535598	6	4	theme	coli	709:712	arg1	cells					683:687	living cells	676:687	living cells of S. aureus and E. coli bacteria	676:721	Studies showed that all composites exhibit significant capability to reduce living cells of S. aureus and E. coli bacteria at least 60%.
33535598	7	5	theme	prepared	790:797	arg1	method					799:804	the prepared method	786:804	the prepared method	786:804	The simplicity, low cost, and reproducibility of the prepared method indicates the potential to be scaled up for industrial applications.
33535598	1	6	theme	fibers	156:161	arg1	performance					131:141	antibacterial performance	117:141	antibacterial performance of cellulose fibers for paper sheet application	117:189	Here, we aimed to boost antibacterial performance of cellulose fibers for paper sheet application.
33535598	5	7	dep	aureus	562:567	arg1	bacteria					590:597	bacteria	590:597	bacteria	590:597	Antibacterial performance has been studied against Staphylococcus aureus and Escherichia coli bacteria.
33535598	4	8	theme	wt	466:467	arg1	%					479:479	1.0 wt% to 8.0 wt%	462:479	1.0 wt% to 8.0 wt%	462:479	We tested cellulose paper enriched by TiO2 from 1.0 wt% to 8.0 wt%, respectively.
33535598	4	9	theme	1.0	462:464	arg1	wt					466:467	wt	466:467	wt	466:467	We tested cellulose paper enriched by TiO2 from 1.0 wt% to 8.0 wt%, respectively.
33535598	6	10	dep	aureus	695:700	arg1	bacteria					714:721	bacteria	714:721	bacteria	714:721	Studies showed that all composites exhibit significant capability to reduce living cells of S. aureus and E. coli bacteria at least 60%.
33535598	6	11	theme	aureus	695:700	arg1	cells					683:687	living cells	676:687	living cells of S. aureus and E. coli bacteria	676:721	Studies showed that all composites exhibit significant capability to reduce living cells of S. aureus and E. coli bacteria at least 60%.
33535598	1	12	theme	paper	167:171	arg1	application					179:189	paper sheet application	167:189	paper sheet application	167:189	Here, we aimed to boost antibacterial performance of cellulose fibers for paper sheet application.
33535598	3	13	theme	assisted	376:383	arg1	stirring					385:392	mechanical assisted stirring	365:392	mechanical assisted stirring	365:392	A simple and facile composite preparation route based on ultrasound and mechanical assisted stirring has been developed.
33535598	3	14	theme	facile	306:311	arg1	route					335:339	A simple and facile composite preparation route	293:339	A simple and facile composite preparation route based on ultrasound and mechanical assisted stirring	293:392	A simple and facile composite preparation route based on ultrasound and mechanical assisted stirring has been developed.
33535598	1	15	theme	sheet	173:177	arg1	application					179:189	paper sheet application	167:189	paper sheet application	167:189	Here, we aimed to boost antibacterial performance of cellulose fibers for paper sheet application.
33535598	7	16	theme	method	799:804	arg1	simplicity					741:750	simplicity	741:750	simplicity	741:750	The simplicity, low cost, and reproducibility of the prepared method indicates the potential to be scaled up for industrial applications.
33535598	7	16	theme	method	799:804	arg1	cost					757:760	low cost	753:760	low cost	753:760	The simplicity, low cost, and reproducibility of the prepared method indicates the potential to be scaled up for industrial applications.
33535598	7	16	theme	method	799:804	arg1	reproducibility					767:781	reproducibility	767:781	reproducibility	767:781	The simplicity, low cost, and reproducibility of the prepared method indicates the potential to be scaled up for industrial applications.
33535598	0	17	theme	Performance	26:36	arg1	Boosting					0:7	Boosting	0:7	Boosting of Antibacterial Performance of Cellulose Based Paper Sheet via TiO2 Nanoparticles.	0:91	Boosting of Antibacterial Performance of Cellulose Based Paper Sheet via TiO2 Nanoparticles.
33535598	3	18	theme	mechanical	365:374	arg1	stirring					385:392	mechanical assisted stirring	365:392	mechanical assisted stirring	365:392	A simple and facile composite preparation route based on ultrasound and mechanical assisted stirring has been developed.
33535598	2	19	theme	fibers	285:290	arg1	surface					270:276	the surface	266:276	the surface of the fibers	266:290	Therefore, TiO2 nanoparticles have been used with controlled loading onto the surface of the fibers.
33535598	6	20	theme	living	676:681	arg1	cells					683:687	living cells	676:687	living cells of S. aureus and E. coli bacteria	676:721	Studies showed that all composites exhibit significant capability to reduce living cells of S. aureus and E. coli bacteria at least 60%.
33535598	0	21	theme	Cellulose	41:49	arg1	Sheet					63:67	Cellulose Based Paper Sheet	41:67	Cellulose Based Paper Sheet	41:67	Boosting of Antibacterial Performance of Cellulose Based Paper Sheet via TiO2 Nanoparticles.
33535598	2	22	used	used	232:235	arg2	nanoparticles					208:220	TiO2 nanoparticles	203:220	TiO2 nanoparticles	203:220	Therefore, TiO2 nanoparticles have been used with controlled loading onto the surface of the fibers.
33535598	7	23	theme	low	753:755	arg1	cost					757:760	low cost	753:760	low cost	753:760	The simplicity, low cost, and reproducibility of the prepared method indicates the potential to be scaled up for industrial applications.
33535598	0	24	theme	Paper	57:61	arg1	Sheet					63:67	Cellulose Based Paper Sheet	41:67	Cellulose Based Paper Sheet	41:67	Boosting of Antibacterial Performance of Cellulose Based Paper Sheet via TiO2 Nanoparticles.
33535598	4	25	dep	%	479:479	arg1	to					470:471	to	470:471	to	470:471	We tested cellulose paper enriched by TiO2 from 1.0 wt% to 8.0 wt%, respectively.
33535598	0	26	theme	Sheet	63:67	arg1	Performance					26:36	Antibacterial Performance	12:36	Antibacterial Performance of Cellulose Based Paper Sheet	12:67	Boosting of Antibacterial Performance of Cellulose Based Paper Sheet via TiO2 Nanoparticles.
33535598	0	27	theme	Based	51:55	arg1	Sheet					63:67	Cellulose Based Paper Sheet	41:67	Cellulose Based Paper Sheet	41:67	Boosting of Antibacterial Performance of Cellulose Based Paper Sheet via TiO2 Nanoparticles.
33535598	3	28	theme	simple	295:300	arg1	route					335:339	A simple and facile composite preparation route	293:339	A simple and facile composite preparation route based on ultrasound and mechanical assisted stirring	293:392	A simple and facile composite preparation route based on ultrasound and mechanical assisted stirring has been developed.
33535598	2	29	theme	TiO2	203:206	arg1	nanoparticles					208:220	TiO2 nanoparticles	203:220	TiO2 nanoparticles	203:220	Therefore, TiO2 nanoparticles have been used with controlled loading onto the surface of the fibers.
33535598	1	30	theme	antibacterial	117:129	arg1	performance					131:141	antibacterial performance	117:141	antibacterial performance of cellulose fibers for paper sheet application	117:189	Here, we aimed to boost antibacterial performance of cellulose fibers for paper sheet application.
33535598	6	31	theme	significant	643:653	arg1	capability					655:664	significant capability	643:664	significant capability to reduce living cells of S. aureus and E. coli bacteria at least 60%	643:734	Studies showed that all composites exhibit significant capability to reduce living cells of S. aureus and E. coli bacteria at least 60%.
33535598	3	32	theme	composite	313:321	arg1	route					335:339	A simple and facile composite preparation route	293:339	A simple and facile composite preparation route based on ultrasound and mechanical assisted stirring	293:392	A simple and facile composite preparation route based on ultrasound and mechanical assisted stirring has been developed.
33535598	4	33	theme	cellulose	424:432	arg1	paper					434:438	cellulose paper	424:438	cellulose paper enriched by TiO2 from 1.0 wt% to 8.0 wt%, respectively	424:493	We tested cellulose paper enriched by TiO2 from 1.0 wt% to 8.0 wt%, respectively.
33535598	2	34	theme	controlled	242:251	arg1	loading					253:259	controlled loading	242:259	controlled loading onto the surface of the fibers	242:290	Therefore, TiO2 nanoparticles have been used with controlled loading onto the surface of the fibers.
33535598	3	35	theme	preparation	323:333	arg1	route					335:339	A simple and facile composite preparation route	293:339	A simple and facile composite preparation route based on ultrasound and mechanical assisted stirring	293:392	A simple and facile composite preparation route based on ultrasound and mechanical assisted stirring has been developed.
33535598	4	36	theme	wt	477:478	arg1	%					479:479	1.0 wt% to 8.0 wt%	462:479	1.0 wt% to 8.0 wt%	462:479	We tested cellulose paper enriched by TiO2 from 1.0 wt% to 8.0 wt%, respectively.
33535598	0	37	theme	Antibacterial	12:24	arg1	Performance					26:36	Antibacterial Performance	12:36	Antibacterial Performance of Cellulose Based Paper Sheet	12:67	Boosting of Antibacterial Performance of Cellulose Based Paper Sheet via TiO2 Nanoparticles.
33535598	5	38	theme	Antibacterial	496:508	arg1	performance					510:520	Antibacterial performance	496:520	Antibacterial performance	496:520	Antibacterial performance has been studied against Staphylococcus aureus and Escherichia coli bacteria.
34791937	0	0	theme	graphene	82:89	arg1	layers					91:96	functionalized graphene layers	67:96	functionalized graphene layers	67:96	Bionanocomposites based on a covalent network of chitosan and edge functionalized graphene layers.
34791937	1	1	theme	crosslinked	296:306	arg1	network					308:314	a crosslinked network	294:314	a crosslinked network	294:314	In this study, carbon papers and aerogels were prepared from chitosan and graphene layers with aldehydic edge functional groups (G-CHO) able to form chemical bonds with chitosan and thus to form a crosslinked network.
34791937	11	2	theme	graphene	1564:1571	arg1	layers					1573:1578	graphene layers	1564:1578	graphene layers	1564:1578	This work demonstrates the effectiveness of a continuous covalent network between chitosan and graphene layers edge functionalized with tailor made functional groups for the preparation of carbon papers and aerogels and paves the way for the scale up of such a type of composites.
34791937	2	3	theme	high	319:322	arg1	edge					350:353	edge	350:353	edge functionalized with hydroxyl groups (G-OH)	350:396	A high surface area graphite was edge functionalized with hydroxyl groups (G-OH) through the reaction with KOH.
34791937	2	3	theme	high	319:322	arg1	graphite					337:344	A high surface area graphite	317:344	A high surface area graphite	317:344	A high surface area graphite was edge functionalized with hydroxyl groups (G-OH) through the reaction with KOH.
34791937	7	4	contain	had	1052:1054	arg2	layers					1073:1078	about 22 stacked layers	1056:1078	about 22 stacked layers	1056:1078	G-CHO, which had about 22 stacked layers, was extensively exfoliated in the carbon paper, as confirmed by the absence of the 002 reflection of the graphitic crystallites in the XRD pattern.
34791937	7	4	contain	had	1052:1054	arg1	G-CHO					1039:1043	G-CHO	1039:1043	G-CHO	1039:1043	G-CHO, which had about 22 stacked layers, was extensively exfoliated in the carbon paper, as confirmed by the absence of the 002 reflection of the graphitic crystallites in the XRD pattern.
34791937	0	5	theme	functionalized	67:80	arg1	layers					91:96	functionalized graphene layers	67:96	functionalized graphene layers	67:96	Bionanocomposites based on a covalent network of chitosan and edge functionalized graphene layers.
34791937	11	6	theme	covalent	1526:1533	arg1	network					1535:1541	a continuous covalent network	1513:1541	a continuous covalent network between chitosan and graphene layers edge functionalized with tailor made functional groups for the preparation of carbon papers and aerogels	1513:1683	This work demonstrates the effectiveness of a continuous covalent network between chitosan and graphene layers edge functionalized with tailor made functional groups for the preparation of carbon papers and aerogels and paves the way for the scale up of such a type of composites.
34791937	7	7	theme	stacked	1065:1071	arg1	layers					1073:1078	about 22 stacked layers	1056:1078	about 22 stacked layers	1056:1078	G-CHO, which had about 22 stacked layers, was extensively exfoliated in the carbon paper, as confirmed by the absence of the 002 reflection of the graphitic crystallites in the XRD pattern.
34791937	7	8	theme	002	1164:1166	arg1	reflection					1168:1177	the 002 reflection	1160:1177	the 002 reflection of the graphitic crystallites in the XRD pattern	1160:1226	G-CHO, which had about 22 stacked layers, was extensively exfoliated in the carbon paper, as confirmed by the absence of the 002 reflection of the graphitic crystallites in the XRD pattern.
34791937	1	9	theme	able	235:238	arg1	G-CHO					228:232	G-CHO	228:232	G-CHO	228:232	In this study, carbon papers and aerogels were prepared from chitosan and graphene layers with aldehydic edge functional groups (G-CHO) able to form chemical bonds with chitosan and thus to form a crosslinked network.
34791937	1	9	theme	able	235:238	arg1	groups					220:225	aldehydic edge functional groups	194:225	aldehydic edge functional groups (G-CHO) able to form chemical bonds with chitosan and thus to form a crosslinked network	194:314	In this study, carbon papers and aerogels were prepared from chitosan and graphene layers with aldehydic edge functional groups (G-CHO) able to form chemical bonds with chitosan and thus to form a crosslinked network.
34791937	11	10	theme	network	1535:1541	arg1	effectiveness					1496:1508	the effectiveness	1492:1508	the effectiveness of a continuous covalent network between chitosan and graphene layers edge functionalized with tailor made functional groups for the preparation of carbon papers and aerogels	1492:1683	This work demonstrates the effectiveness of a continuous covalent network between chitosan and graphene layers edge functionalized with tailor made functional groups for the preparation of carbon papers and aerogels and paves the way for the scale up of such a type of composites.
34791937	5	11	theme	acidic	825:830	arg1	suspension					838:847	a stable acidic water suspension	816:847	a stable acidic water suspension	816:847	CS and G-CHO were mixed with mortar and pestle and carbon papers and aerogels were obtained from a stable acidic water suspension through casting and liophilization, respectively.
34791937	7	12	from	exfoliated	1097:1106	arg1	paper					1122:1126	the carbon paper	1111:1126	the carbon paper	1111:1126	G-CHO, which had about 22 stacked layers, was extensively exfoliated in the carbon paper, as confirmed by the absence of the 002 reflection of the graphitic crystallites in the XRD pattern.
34791937	4	13	theme	X-ray	630:634	arg1	analysis					636:643	X-ray analysis	630:643	X-ray analysis	630:643	Characterization of the graphitic materials was performed with elemental analysis, titration, X-ray analysis, Raman spectroscopy and by estimating their Hansen solubility parameters.
34791937	4	13	theme	X-ray	630:634	arg1	analysis					609:616	elemental analysis	599:616	elemental analysis	599:616	Characterization of the graphitic materials was performed with elemental analysis, titration, X-ray analysis, Raman spectroscopy and by estimating their Hansen solubility parameters.
34791937	7	14	from	reflection	1168:1177	arg1	pattern					1220:1226	the XRD pattern	1212:1226	the XRD pattern	1212:1226	G-CHO, which had about 22 stacked layers, was extensively exfoliated in the carbon paper, as confirmed by the absence of the 002 reflection of the graphitic crystallites in the XRD pattern.
34791937	5	15	theme	water	832:836	arg1	suspension					838:847	a stable acidic water suspension	816:847	a stable acidic water suspension	816:847	CS and G-CHO were mixed with mortar and pestle and carbon papers and aerogels were obtained from a stable acidic water suspension through casting and liophilization, respectively.
34791937	7	16	theme	graphitic	1186:1194	arg1	crystallites					1196:1207	the graphitic crystallites	1182:1207	the graphitic crystallites in the XRD pattern	1182:1226	G-CHO, which had about 22 stacked layers, was extensively exfoliated in the carbon paper, as confirmed by the absence of the 002 reflection of the graphitic crystallites in the XRD pattern.
34791937	7	17	theme	reflection	1168:1177	arg1	absence					1149:1155	the absence	1145:1155	the absence of the 002 reflection of the graphitic crystallites in the XRD pattern	1145:1226	G-CHO, which had about 22 stacked layers, was extensively exfoliated in the carbon paper, as confirmed by the absence of the 002 reflection of the graphitic crystallites in the XRD pattern.
34791937	3	18	with	G-CHO	429:433	arg1	mmol/g					445:450	4.5 mmol/g	441:450	4.5 mmol/g of functional group	441:470	G-CHO, with 4.5 mmol/g of functional group, was prepared from G-OH by means of the Reimer-Tieman reaction.
34791937	4	19	theme	materials	570:578	arg1	Characterization					536:551	Characterization	536:551	Characterization of the graphitic materials	536:578	Characterization of the graphitic materials was performed with elemental analysis, titration, X-ray analysis, Raman spectroscopy and by estimating their Hansen solubility parameters.
34791937	6	20	theme	continuous	975:984	arg1	network					995:1001	a continuous covalent network	973:1001	a continuous covalent network between G-CHO and CS	973:1022	Free standing and foldable carbon papers and monolithic aerogels based on a continuous covalent network between G-CHO and CS were prepared.
34791937	9	21	theme	electrical	1329:1338	arg1	conductivity					1340:1351	electrical conductivity	1329:1351	electrical conductivity	1329:1351	Composites revealed electrical conductivity.
34791937	11	22	theme	aerogels	1676:1683	arg1	preparation					1643:1653	the preparation	1639:1653	the preparation of carbon papers and aerogels	1639:1683	This work demonstrates the effectiveness of a continuous covalent network between chitosan and graphene layers edge functionalized with tailor made functional groups for the preparation of carbon papers and aerogels and paves the way for the scale up of such a type of composites.
34791937	11	23	theme	carbon	1658:1663	arg1	papers					1665:1670	carbon papers	1658:1670	carbon papers	1658:1670	This work demonstrates the effectiveness of a continuous covalent network between chitosan and graphene layers edge functionalized with tailor made functional groups for the preparation of carbon papers and aerogels and paves the way for the scale up of such a type of composites.
34791937	4	24	theme	elemental	599:607	arg1	analysis					636:643	X-ray analysis	630:643	X-ray analysis	630:643	Characterization of the graphitic materials was performed with elemental analysis, titration, X-ray analysis, Raman spectroscopy and by estimating their Hansen solubility parameters.
34791937	4	24	theme	elemental	599:607	arg1	analysis					609:616	elemental analysis	599:616	elemental analysis	599:616	Characterization of the graphitic materials was performed with elemental analysis, titration, X-ray analysis, Raman spectroscopy and by estimating their Hansen solubility parameters.
34791937	4	24	theme	elemental	599:607	arg1	titration					619:627	titration	619:627	titration	619:627	Characterization of the graphitic materials was performed with elemental analysis, titration, X-ray analysis, Raman spectroscopy and by estimating their Hansen solubility parameters.
34791937	4	24	theme	elemental	599:607	arg1	spectroscopy					652:663	Raman spectroscopy	646:663	Raman spectroscopy	646:663	Characterization of the graphitic materials was performed with elemental analysis, titration, X-ray analysis, Raman spectroscopy and by estimating their Hansen solubility parameters.
34791937	10	25	theme	IR	1428:1429	arg1	findings					1431:1438	the IR findings	1424:1438	the IR findings	1424:1438	The covalent network between the graphene layers and CS, suggested by the IR findings, accounts for these results.
34791937	6	26	theme	carbon	926:931	arg1	papers					933:938	Free standing and foldable carbon papers	899:938	Free standing and foldable carbon papers	899:938	Free standing and foldable carbon papers and monolithic aerogels based on a continuous covalent network between G-CHO and CS were prepared.
34791937	11	27	theme	type	1730:1733	arg1	up					1717:1718	the scale up	1707:1718	the scale up of such a type of composites	1707:1747	This work demonstrates the effectiveness of a continuous covalent network between chitosan and graphene layers edge functionalized with tailor made functional groups for the preparation of carbon papers and aerogels and paves the way for the scale up of such a type of composites.
34791937	7	28	theme	carbon	1115:1120	arg1	paper					1122:1126	the carbon paper	1111:1126	the carbon paper	1111:1126	G-CHO, which had about 22 stacked layers, was extensively exfoliated in the carbon paper, as confirmed by the absence of the 002 reflection of the graphitic crystallites in the XRD pattern.
34791937	1	29	theme	chemical	248:255	arg1	bonds					257:261	chemical bonds	248:261	chemical bonds	248:261	In this study, carbon papers and aerogels were prepared from chitosan and graphene layers with aldehydic edge functional groups (G-CHO) able to form chemical bonds with chitosan and thus to form a crosslinked network.
34791937	10	30	theme	covalent	1358:1365	arg1	network					1367:1373	The covalent network	1354:1373	The covalent network	1354:1373	The covalent network between the graphene layers and CS, suggested by the IR findings, accounts for these results.
34791937	7	31	from	crystallites	1196:1207	arg1	pattern					1220:1226	the XRD pattern	1212:1226	the XRD pattern	1212:1226	G-CHO, which had about 22 stacked layers, was extensively exfoliated in the carbon paper, as confirmed by the absence of the 002 reflection of the graphitic crystallites in the XRD pattern.
34791937	6	32	theme	foldable	917:924	arg1	papers					933:938	Free standing and foldable carbon papers	899:938	Free standing and foldable carbon papers	899:938	Free standing and foldable carbon papers and monolithic aerogels based on a continuous covalent network between G-CHO and CS were prepared.
34791937	1	33	dep	chitosan	160:167	arg1	layers					182:187	layers	182:187	layers	182:187	In this study, carbon papers and aerogels were prepared from chitosan and graphene layers with aldehydic edge functional groups (G-CHO) able to form chemical bonds with chitosan and thus to form a crosslinked network.
34791937	4	34	theme	solubility	696:705	arg1	parameters					707:716	their Hansen solubility parameters	683:716	their Hansen solubility parameters	683:716	Characterization of the graphitic materials was performed with elemental analysis, titration, X-ray analysis, Raman spectroscopy and by estimating their Hansen solubility parameters.
34791937	5	35	theme	carbon	770:775	arg1	papers					777:782	carbon papers	770:782	carbon papers	770:782	CS and G-CHO were mixed with mortar and pestle and carbon papers and aerogels were obtained from a stable acidic water suspension through casting and liophilization, respectively.
34791937	11	36	theme	continuous	1515:1524	arg1	network					1535:1541	a continuous covalent network	1513:1541	a continuous covalent network between chitosan and graphene layers edge functionalized with tailor made functional groups for the preparation of carbon papers and aerogels	1513:1683	This work demonstrates the effectiveness of a continuous covalent network between chitosan and graphene layers edge functionalized with tailor made functional groups for the preparation of carbon papers and aerogels and paves the way for the scale up of such a type of composites.
34791937	7	37	from	paper	1122:1126	arg1	exfoliated					1097:1106	exfoliated	1097:1106	exfoliated	1097:1106	G-CHO, which had about 22 stacked layers, was extensively exfoliated in the carbon paper, as confirmed by the absence of the 002 reflection of the graphitic crystallites in the XRD pattern.
34791937	4	38	theme	Hansen	689:694	arg1	parameters					707:716	their Hansen solubility parameters	683:716	their Hansen solubility parameters	683:716	Characterization of the graphitic materials was performed with elemental analysis, titration, X-ray analysis, Raman spectroscopy and by estimating their Hansen solubility parameters.
34791937	3	39	theme	functional	455:464	arg1	group					466:470	functional group	455:470	functional group	455:470	G-CHO, with 4.5 mmol/g of functional group, was prepared from G-OH by means of the Reimer-Tieman reaction.
34791937	7	40	theme	XRD	1216:1218	arg1	pattern					1220:1226	the XRD pattern	1212:1226	the XRD pattern	1212:1226	G-CHO, which had about 22 stacked layers, was extensively exfoliated in the carbon paper, as confirmed by the absence of the 002 reflection of the graphitic crystallites in the XRD pattern.
34791937	6	41	theme	standing	904:911	arg1	papers					933:938	Free standing and foldable carbon papers	899:938	Free standing and foldable carbon papers	899:938	Free standing and foldable carbon papers and monolithic aerogels based on a continuous covalent network between G-CHO and CS were prepared.
34791937	1	42	theme	aldehydic	194:202	arg1	G-CHO					228:232	G-CHO	228:232	G-CHO	228:232	In this study, carbon papers and aerogels were prepared from chitosan and graphene layers with aldehydic edge functional groups (G-CHO) able to form chemical bonds with chitosan and thus to form a crosslinked network.
34791937	1	42	theme	aldehydic	194:202	arg1	groups					220:225	aldehydic edge functional groups	194:225	aldehydic edge functional groups (G-CHO) able to form chemical bonds with chitosan and thus to form a crosslinked network	194:314	In this study, carbon papers and aerogels were prepared from chitosan and graphene layers with aldehydic edge functional groups (G-CHO) able to form chemical bonds with chitosan and thus to form a crosslinked network.
34791937	0	43	dep	chitosan	49:56	arg1	layers					91:96	functionalized graphene layers	67:96	functionalized graphene layers	67:96	Bionanocomposites based on a covalent network of chitosan and edge functionalized graphene layers.
34791937	1	44	link	crosslinked	296:306	arg1	network					308:314	a crosslinked network	294:314	a crosslinked network	294:314	In this study, carbon papers and aerogels were prepared from chitosan and graphene layers with aldehydic edge functional groups (G-CHO) able to form chemical bonds with chitosan and thus to form a crosslinked network.
34791937	2	45	theme	hydroxyl	375:382	arg1	G-OH					392:395	G-OH	392:395	G-OH	392:395	A high surface area graphite was edge functionalized with hydroxyl groups (G-OH) through the reaction with KOH.
34791937	2	45	theme	hydroxyl	375:382	arg1	groups					384:389	hydroxyl groups	375:389	hydroxyl groups (G-OH)	375:396	A high surface area graphite was edge functionalized with hydroxyl groups (G-OH) through the reaction with KOH.
34791937	0	46	theme	covalent	29:36	arg1	network					38:44	a covalent network	27:44	a covalent network of chitosan and edge functionalized graphene layers	27:96	Bionanocomposites based on a covalent network of chitosan and edge functionalized graphene layers.
34791937	6	47	theme	Free	899:902	arg1	papers					933:938	Free standing and foldable carbon papers	899:938	Free standing and foldable carbon papers	899:938	Free standing and foldable carbon papers and monolithic aerogels based on a continuous covalent network between G-CHO and CS were prepared.
34791937	6	48	theme	monolithic	944:953	arg1	aerogels					955:962	monolithic aerogels	944:962	monolithic aerogels	944:962	Free standing and foldable carbon papers and monolithic aerogels based on a continuous covalent network between G-CHO and CS were prepared.
34791937	8	49	theme	Carbon	1229:1234	arg1	paper					1236:1240	Carbon paper	1229:1240	Carbon paper	1229:1240	Carbon paper was found to be resistant to solvents and to be stable for pH ⩾ 7.
34791937	1	50	theme	edge	204:207	arg1	G-CHO					228:232	G-CHO	228:232	G-CHO	228:232	In this study, carbon papers and aerogels were prepared from chitosan and graphene layers with aldehydic edge functional groups (G-CHO) able to form chemical bonds with chitosan and thus to form a crosslinked network.
34791937	1	50	theme	edge	204:207	arg1	groups					220:225	aldehydic edge functional groups	194:225	aldehydic edge functional groups (G-CHO) able to form chemical bonds with chitosan and thus to form a crosslinked network	194:314	In this study, carbon papers and aerogels were prepared from chitosan and graphene layers with aldehydic edge functional groups (G-CHO) able to form chemical bonds with chitosan and thus to form a crosslinked network.
34791937	0	51	theme	chitosan	49:56	arg1	network					38:44	a covalent network	27:44	a covalent network of chitosan and edge functionalized graphene layers	27:96	Bionanocomposites based on a covalent network of chitosan and edge functionalized graphene layers.
34791937	5	52	theme	stable	818:823	arg1	suspension					838:847	a stable acidic water suspension	816:847	a stable acidic water suspension	816:847	CS and G-CHO were mixed with mortar and pestle and carbon papers and aerogels were obtained from a stable acidic water suspension through casting and liophilization, respectively.
34791937	6	53	theme	covalent	986:993	arg1	network					995:1001	a continuous covalent network	973:1001	a continuous covalent network between G-CHO and CS	973:1022	Free standing and foldable carbon papers and monolithic aerogels based on a continuous covalent network between G-CHO and CS were prepared.
34791937	7	54	theme	crystallites	1196:1207	arg1	reflection					1168:1177	the 002 reflection	1160:1177	the 002 reflection of the graphitic crystallites in the XRD pattern	1160:1226	G-CHO, which had about 22 stacked layers, was extensively exfoliated in the carbon paper, as confirmed by the absence of the 002 reflection of the graphitic crystallites in the XRD pattern.
34791937	10	55	theme	graphene	1387:1394	arg1	layers					1396:1401	the graphene layers	1383:1401	the graphene layers	1383:1401	The covalent network between the graphene layers and CS, suggested by the IR findings, accounts for these results.
34791937	11	56	theme	scale	1711:1715	arg1	up					1717:1718	the scale up	1707:1718	the scale up of such a type of composites	1707:1747	This work demonstrates the effectiveness of a continuous covalent network between chitosan and graphene layers edge functionalized with tailor made functional groups for the preparation of carbon papers and aerogels and paves the way for the scale up of such a type of composites.
34791937	3	57	theme	Reimer-Tieman	512:524	arg1	reaction					526:533	the Reimer-Tieman reaction	508:533	the Reimer-Tieman reaction	508:533	G-CHO, with 4.5 mmol/g of functional group, was prepared from G-OH by means of the Reimer-Tieman reaction.
34791937	7	58	from	pattern	1220:1226	arg1	reflection					1168:1177	the 002 reflection	1160:1177	the 002 reflection of the graphitic crystallites in the XRD pattern	1160:1226	G-CHO, which had about 22 stacked layers, was extensively exfoliated in the carbon paper, as confirmed by the absence of the 002 reflection of the graphitic crystallites in the XRD pattern.
34791937	4	59	theme	Raman	646:650	arg1	spectroscopy					652:663	Raman spectroscopy	646:663	Raman spectroscopy	646:663	Characterization of the graphitic materials was performed with elemental analysis, titration, X-ray analysis, Raman spectroscopy and by estimating their Hansen solubility parameters.
34791937	4	59	theme	Raman	646:650	arg1	analysis					609:616	elemental analysis	599:616	elemental analysis	599:616	Characterization of the graphitic materials was performed with elemental analysis, titration, X-ray analysis, Raman spectroscopy and by estimating their Hansen solubility parameters.
34791937	11	60	theme	composites	1738:1747	arg1	type					1730:1733	such a type	1723:1733	such a type of composites	1723:1747	This work demonstrates the effectiveness of a continuous covalent network between chitosan and graphene layers edge functionalized with tailor made functional groups for the preparation of carbon papers and aerogels and paves the way for the scale up of such a type of composites.
34791937	11	61	dep	chitosan	1551:1558	arg1	edge					1580:1583	edge	1580:1583	edge	1580:1583	This work demonstrates the effectiveness of a continuous covalent network between chitosan and graphene layers edge functionalized with tailor made functional groups for the preparation of carbon papers and aerogels and paves the way for the scale up of such a type of composites.
34791937	1	62	theme	carbon	114:119	arg1	papers					121:126	carbon papers	114:126	carbon papers	114:126	In this study, carbon papers and aerogels were prepared from chitosan and graphene layers with aldehydic edge functional groups (G-CHO) able to form chemical bonds with chitosan and thus to form a crosslinked network.
34791937	1	63	theme	functional	209:218	arg1	G-CHO					228:232	G-CHO	228:232	G-CHO	228:232	In this study, carbon papers and aerogels were prepared from chitosan and graphene layers with aldehydic edge functional groups (G-CHO) able to form chemical bonds with chitosan and thus to form a crosslinked network.
34791937	1	63	theme	functional	209:218	arg1	groups					220:225	aldehydic edge functional groups	194:225	aldehydic edge functional groups (G-CHO) able to form chemical bonds with chitosan and thus to form a crosslinked network	194:314	In this study, carbon papers and aerogels were prepared from chitosan and graphene layers with aldehydic edge functional groups (G-CHO) able to form chemical bonds with chitosan and thus to form a crosslinked network.
34791937	11	64	theme	functional	1617:1626	arg1	groups					1628:1633	functional groups	1617:1633	functional groups	1617:1633	This work demonstrates the effectiveness of a continuous covalent network between chitosan and graphene layers edge functionalized with tailor made functional groups for the preparation of carbon papers and aerogels and paves the way for the scale up of such a type of composites.
34791937	3	65	theme	group	466:470	arg1	mmol/g					445:450	4.5 mmol/g	441:450	4.5 mmol/g of functional group	441:470	G-CHO, with 4.5 mmol/g of functional group, was prepared from G-OH by means of the Reimer-Tieman reaction.
34791937	11	66	theme	papers	1665:1670	arg1	preparation					1643:1653	the preparation	1639:1653	the preparation of carbon papers and aerogels	1639:1683	This work demonstrates the effectiveness of a continuous covalent network between chitosan and graphene layers edge functionalized with tailor made functional groups for the preparation of carbon papers and aerogels and paves the way for the scale up of such a type of composites.
34791937	0	67	theme	edge	62:65	arg1	network					38:44	a covalent network	27:44	a covalent network of chitosan and edge functionalized graphene layers	27:96	Bionanocomposites based on a covalent network of chitosan and edge functionalized graphene layers.
34791937	1	68	dep	able	235:238	arg1	form					243:246	form	243:246	to form chemical bonds with chitosan	240:275	In this study, carbon papers and aerogels were prepared from chitosan and graphene layers with aldehydic edge functional groups (G-CHO) able to form chemical bonds with chitosan and thus to form a crosslinked network.
34791937	1	68	dep	able	235:238	arg1	form					289:292	form	289:292	thus to form a crosslinked network	281:314	In this study, carbon papers and aerogels were prepared from chitosan and graphene layers with aldehydic edge functional groups (G-CHO) able to form chemical bonds with chitosan and thus to form a crosslinked network.
34791937	2	69	with	reaction	410:417	arg1	KOH					424:426	KOH	424:426	KOH	424:426	A high surface area graphite was edge functionalized with hydroxyl groups (G-OH) through the reaction with KOH.
34791937	2	70	theme	area	332:335	arg1	edge					350:353	edge	350:353	edge functionalized with hydroxyl groups (G-OH)	350:396	A high surface area graphite was edge functionalized with hydroxyl groups (G-OH) through the reaction with KOH.
34791937	2	70	theme	area	332:335	arg1	graphite					337:344	A high surface area graphite	317:344	A high surface area graphite	317:344	A high surface area graphite was edge functionalized with hydroxyl groups (G-OH) through the reaction with KOH.
34791937	4	71	theme	graphitic	560:568	arg1	materials					570:578	the graphitic materials	556:578	the graphitic materials	556:578	Characterization of the graphitic materials was performed with elemental analysis, titration, X-ray analysis, Raman spectroscopy and by estimating their Hansen solubility parameters.
34791937	2	72	theme	surface	324:330	arg1	edge					350:353	edge	350:353	edge functionalized with hydroxyl groups (G-OH)	350:396	A high surface area graphite was edge functionalized with hydroxyl groups (G-OH) through the reaction with KOH.
34791937	2	72	theme	surface	324:330	arg1	graphite					337:344	A high surface area graphite	317:344	A high surface area graphite	317:344	A high surface area graphite was edge functionalized with hydroxyl groups (G-OH) through the reaction with KOH.
34791937	8	73	theme	pH	1301:1302	arg1	⩾					1304:1304	pH ⩾ 7	1301:1306	pH ⩾ 7	1301:1306	Carbon paper was found to be resistant to solvents and to be stable for pH ⩾ 7.
32100808	0	0	from	actuation	14:22	arg1	hydrogels					78:86	stiffness adjustable bacterial cellulose composite hydrogels	27:86	stiffness adjustable bacterial cellulose composite hydrogels	27:86	Osmotic squat actuation in stiffness adjustable bacterial cellulose composite hydrogels.
32100808	1	1	theme	external	180:187	arg1	stimuli					189:195	external stimuli	180:195	external stimuli	180:195	Mechanically adaptive hydrogels can change their mechanical characteristics in response to external stimuli and have potential applications in biomechanical fields.
32100808	8	2	theme	smart	1643:1647	arg1	structures					1649:1658	smart structures	1643:1658	smart structures	1643:1658	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability are expected to provide more options in developing novel bioimplants and smart structures.
32100808	3	3	theme	compression	662:672	arg1	%					694:694	compression strain lower than 27%	662:694	compression strain lower than 27% at a stress of 0.1 MPa	662:717	The obtained BC-g-PAA composite hydrogels showed adjustable stiffness in compression, remained soft at pH lower than 6 (compression strain over 49% at a stress of 0.1 MPa), and stiffened when pH reached 7 (compression strain lower than 27% at a stress of 0.1 MPa), while the volume change ratio was consistently lower than 15%.
32100808	8	4	theme	pH-responsive	1420:1432	arg1	hydrogels					1469:1477	The developed pH-responsive mechanically adaptive BC composite hydrogels	1406:1477	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability	1406:1568	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability are expected to provide more options in developing novel bioimplants and smart structures.
32100808	8	5	theme	dimensional	1489:1499	arg1	stability					1501:1509	high dimensional stability	1484:1509	high dimensional stability	1484:1509	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability are expected to provide more options in developing novel bioimplants and smart structures.
32100808	4	6	theme	a	856:856	arg1	weight					858:863	lift a weight	851:863	lift a weight	851:863	Based on this, the hydrogels showed interesting squat actuation to lift a weight.
32100808	5	7	theme	fabricating	1049:1059	arg1	hydrogels					1109:1117	fabricating volumetrically stable and mechanically adaptive hydrogels	1049:1117	fabricating volumetrically stable and mechanically adaptive hydrogels	1049:1117	The BC composite hydrogels exhibited dual pH-responsiveness after grafting PAA with poly[2-(dimethylamino)ethyl methacrylate], confirming the general availability of this strategy in fabricating volumetrically stable and mechanically adaptive hydrogels.
32100808	3	8	theme	change	738:743	arg1	ratio					745:749	the volume change ratio	727:749	the volume change ratio	727:749	The obtained BC-g-PAA composite hydrogels showed adjustable stiffness in compression, remained soft at pH lower than 6 (compression strain over 49% at a stress of 0.1 MPa), and stiffened when pH reached 7 (compression strain lower than 27% at a stress of 0.1 MPa), while the volume change ratio was consistently lower than 15%.
32100808	3	8	theme	change	738:743	arg1	lower					768:772	lower	768:772	lower	768:772	The obtained BC-g-PAA composite hydrogels showed adjustable stiffness in compression, remained soft at pH lower than 6 (compression strain over 49% at a stress of 0.1 MPa), and stiffened when pH reached 7 (compression strain lower than 27% at a stress of 0.1 MPa), while the volume change ratio was consistently lower than 15%.
32100808	4	9	theme	lift	851:854	arg1	weight					858:863	lift a weight	851:863	lift a weight	851:863	Based on this, the hydrogels showed interesting squat actuation to lift a weight.
32100808	8	10	theme	UV-activated	1515:1526	arg1	squat					1543:1547	UV-activated spatiotemporal squat	1515:1547	UV-activated spatiotemporal squat actuating capability	1515:1568	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability are expected to provide more options in developing novel bioimplants and smart structures.
32100808	6	11	theme	UV	1217:1218	arg1	irradiation					1220:1230	UV irradiation	1217:1230	UV irradiation	1217:1230	The surrounding solution-independent softening of BC-g-PAA hydrogels was observed in 8 min under UV irradiation via a photo-triggered pH jump reaction.
32100808	5	12	theme	general	1008:1014	arg1	availability					1016:1027	the general availability	1004:1027	the general availability of this strategy in fabricating volumetrically stable and mechanically adaptive hydrogels	1004:1117	The BC composite hydrogels exhibited dual pH-responsiveness after grafting PAA with poly[2-(dimethylamino)ethyl methacrylate], confirming the general availability of this strategy in fabricating volumetrically stable and mechanically adaptive hydrogels.
32100808	3	13	theme	composite	478:486	arg1	hydrogels					488:496	The obtained BC-g-PAA composite hydrogels	456:496	The obtained BC-g-PAA composite hydrogels	456:496	The obtained BC-g-PAA composite hydrogels showed adjustable stiffness in compression, remained soft at pH lower than 6 (compression strain over 49% at a stress of 0.1 MPa), and stiffened when pH reached 7 (compression strain lower than 27% at a stress of 0.1 MPa), while the volume change ratio was consistently lower than 15%.
32100808	5	14	theme	composite	873:881	arg1	hydrogels					883:891	The BC composite hydrogels	866:891	The BC composite hydrogels	866:891	The BC composite hydrogels exhibited dual pH-responsiveness after grafting PAA with poly[2-(dimethylamino)ethyl methacrylate], confirming the general availability of this strategy in fabricating volumetrically stable and mechanically adaptive hydrogels.
32100808	2	15	theme	bacterial	399:407	arg1	BC					420:421	BC	420:421	BC	420:421	To eliminate the undesired swelling/shrinkage in the responding process, poly(acrylic acid) (PAA) was grafted to acryloyl chloride (AC)-modified bacterial cellulose (BC) by free-radical polymerization.
32100808	2	15	theme	bacterial	399:407	arg1	cellulose					409:417	bacterial cellulose	399:417	acryloyl chloride (AC)-modified bacterial cellulose (BC)	367:422	To eliminate the undesired swelling/shrinkage in the responding process, poly(acrylic acid) (PAA) was grafted to acryloyl chloride (AC)-modified bacterial cellulose (BC) by free-radical polymerization.
32100808	7	16	with	softening	1345:1353	arg1	actuation					1360:1368	actuation	1360:1368	actuation	1360:1368	By virtue of the selective UV irradiation, spatiotemporally controllable softening with actuation in BC-g-PAA hydrogels was realized.
32100808	3	17	theme	strain	674:679	arg1	%					694:694	compression strain lower than 27%	662:694	compression strain lower than 27% at a stress of 0.1 MPa	662:717	The obtained BC-g-PAA composite hydrogels showed adjustable stiffness in compression, remained soft at pH lower than 6 (compression strain over 49% at a stress of 0.1 MPa), and stiffened when pH reached 7 (compression strain lower than 27% at a stress of 0.1 MPa), while the volume change ratio was consistently lower than 15%.
32100808	1	18	from	characteristics	149:163	arg1	response					168:175	response	168:175	response to external stimuli	168:195	Mechanically adaptive hydrogels can change their mechanical characteristics in response to external stimuli and have potential applications in biomechanical fields.
32100808	1	19	theme	Mechanically	89:100	arg1	hydrogels					111:119	Mechanically adaptive hydrogels	89:119	Mechanically adaptive hydrogels	89:119	Mechanically adaptive hydrogels can change their mechanical characteristics in response to external stimuli and have potential applications in biomechanical fields.
32100808	3	20	from	stress	609:614	arg1	%					602:602	49%	600:602	49% at a stress of 0.1 MPa	600:625	The obtained BC-g-PAA composite hydrogels showed adjustable stiffness in compression, remained soft at pH lower than 6 (compression strain over 49% at a stress of 0.1 MPa), and stiffened when pH reached 7 (compression strain lower than 27% at a stress of 0.1 MPa), while the volume change ratio was consistently lower than 15%.
32100808	2	21	theme	-modified	389:397	arg1	BC					420:421	BC	420:421	BC	420:421	To eliminate the undesired swelling/shrinkage in the responding process, poly(acrylic acid) (PAA) was grafted to acryloyl chloride (AC)-modified bacterial cellulose (BC) by free-radical polymerization.
32100808	2	21	theme	-modified	389:397	arg1	cellulose					409:417	bacterial cellulose	399:417	acryloyl chloride (AC)-modified bacterial cellulose (BC)	367:422	To eliminate the undesired swelling/shrinkage in the responding process, poly(acrylic acid) (PAA) was grafted to acryloyl chloride (AC)-modified bacterial cellulose (BC) by free-radical polymerization.
32100808	0	22	theme	squat	8:12	arg1	actuation					14:22	Osmotic squat actuation	0:22	Osmotic squat actuation in stiffness adjustable bacterial cellulose composite hydrogels.	0:87	Osmotic squat actuation in stiffness adjustable bacterial cellulose composite hydrogels.
32100808	8	23	theme	spatiotemporal	1528:1541	arg1	squat					1543:1547	UV-activated spatiotemporal squat	1515:1547	UV-activated spatiotemporal squat actuating capability	1515:1568	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability are expected to provide more options in developing novel bioimplants and smart structures.
32100808	1	24	theme	adaptive	102:109	arg1	hydrogels					111:119	Mechanically adaptive hydrogels	89:119	Mechanically adaptive hydrogels	89:119	Mechanically adaptive hydrogels can change their mechanical characteristics in response to external stimuli and have potential applications in biomechanical fields.
32100808	8	25	theme	novel	1621:1625	arg1	bioimplants					1627:1637	novel bioimplants	1621:1637	novel bioimplants	1621:1637	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability are expected to provide more options in developing novel bioimplants and smart structures.
32100808	1	26	theme	potential	206:214	arg1	applications					216:227	potential applications	206:227	potential applications	206:227	Mechanically adaptive hydrogels can change their mechanical characteristics in response to external stimuli and have potential applications in biomechanical fields.
32100808	0	27	theme	Osmotic	0:6	arg1	actuation					14:22	Osmotic squat actuation	0:22	Osmotic squat actuation in stiffness adjustable bacterial cellulose composite hydrogels.	0:87	Osmotic squat actuation in stiffness adjustable bacterial cellulose composite hydrogels.
32100808	5	28	theme	adaptive	1100:1107	arg1	hydrogels					1109:1117	fabricating volumetrically stable and mechanically adaptive hydrogels	1049:1117	fabricating volumetrically stable and mechanically adaptive hydrogels	1049:1117	The BC composite hydrogels exhibited dual pH-responsiveness after grafting PAA with poly[2-(dimethylamino)ethyl methacrylate], confirming the general availability of this strategy in fabricating volumetrically stable and mechanically adaptive hydrogels.
32100808	3	29	from	stress	701:706	arg1	%					694:694	compression strain lower than 27%	662:694	compression strain lower than 27% at a stress of 0.1 MPa	662:717	The obtained BC-g-PAA composite hydrogels showed adjustable stiffness in compression, remained soft at pH lower than 6 (compression strain over 49% at a stress of 0.1 MPa), and stiffened when pH reached 7 (compression strain lower than 27% at a stress of 0.1 MPa), while the volume change ratio was consistently lower than 15%.
32100808	7	30	dep	irradiation	1302:1312	arg1	virtue					1275:1280	virtue	1275:1280	virtue	1275:1280	By virtue of the selective UV irradiation, spatiotemporally controllable softening with actuation in BC-g-PAA hydrogels was realized.
32100808	3	31	theme	compression	576:586	arg1	strain					588:593	compression strain	576:593	compression strain over 49% at a stress of 0.1 MPa	576:625	The obtained BC-g-PAA composite hydrogels showed adjustable stiffness in compression, remained soft at pH lower than 6 (compression strain over 49% at a stress of 0.1 MPa), and stiffened when pH reached 7 (compression strain lower than 27% at a stress of 0.1 MPa), while the volume change ratio was consistently lower than 15%.
32100808	2	32	theme	free-radical	427:438	arg1	polymerization					440:453	free-radical polymerization	427:453	free-radical polymerization	427:453	To eliminate the undesired swelling/shrinkage in the responding process, poly(acrylic acid) (PAA) was grafted to acryloyl chloride (AC)-modified bacterial cellulose (BC) by free-radical polymerization.
32100808	3	33	dep	7	659:659	arg1	%					694:694	compression strain lower than 27%	662:694	compression strain lower than 27% at a stress of 0.1 MPa	662:717	The obtained BC-g-PAA composite hydrogels showed adjustable stiffness in compression, remained soft at pH lower than 6 (compression strain over 49% at a stress of 0.1 MPa), and stiffened when pH reached 7 (compression strain lower than 27% at a stress of 0.1 MPa), while the volume change ratio was consistently lower than 15%.
32100808	4	34	theme	squat	832:836	arg1	actuation					838:846	interesting squat actuation	820:846	interesting squat actuation to lift a weight	820:863	Based on this, the hydrogels showed interesting squat actuation to lift a weight.
32100808	6	35	theme	BC-g-PAA	1170:1177	arg1	hydrogels					1179:1187	BC-g-PAA hydrogels	1170:1187	BC-g-PAA hydrogels	1170:1187	The surrounding solution-independent softening of BC-g-PAA hydrogels was observed in 8 min under UV irradiation via a photo-triggered pH jump reaction.
32100808	2	36	from	swelling/shrinkage	281:298	arg1	process					318:324	the responding process	303:324	the responding process	303:324	To eliminate the undesired swelling/shrinkage in the responding process, poly(acrylic acid) (PAA) was grafted to acryloyl chloride (AC)-modified bacterial cellulose (BC) by free-radical polymerization.
32100808	3	37	theme	BC-g-PAA	469:476	arg1	hydrogels					488:496	The obtained BC-g-PAA composite hydrogels	456:496	The obtained BC-g-PAA composite hydrogels	456:496	The obtained BC-g-PAA composite hydrogels showed adjustable stiffness in compression, remained soft at pH lower than 6 (compression strain over 49% at a stress of 0.1 MPa), and stiffened when pH reached 7 (compression strain lower than 27% at a stress of 0.1 MPa), while the volume change ratio was consistently lower than 15%.
32100808	7	38	theme	BC-g-PAA	1373:1380	arg1	hydrogels					1382:1390	BC-g-PAA hydrogels	1373:1390	BC-g-PAA hydrogels	1373:1390	By virtue of the selective UV irradiation, spatiotemporally controllable softening with actuation in BC-g-PAA hydrogels was realized.
32100808	5	39	theme	strategy	1037:1044	arg1	availability					1016:1027	the general availability	1004:1027	the general availability of this strategy in fabricating volumetrically stable and mechanically adaptive hydrogels	1004:1117	The BC composite hydrogels exhibited dual pH-responsiveness after grafting PAA with poly[2-(dimethylamino)ethyl methacrylate], confirming the general availability of this strategy in fabricating volumetrically stable and mechanically adaptive hydrogels.
32100808	4	40	theme	interesting	820:830	arg1	actuation					838:846	interesting squat actuation	820:846	interesting squat actuation to lift a weight	820:863	Based on this, the hydrogels showed interesting squat actuation to lift a weight.
32100808	8	41	theme	developed	1410:1418	arg1	hydrogels					1469:1477	The developed pH-responsive mechanically adaptive BC composite hydrogels	1406:1477	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability	1406:1568	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability are expected to provide more options in developing novel bioimplants and smart structures.
32100808	2	42	theme	acrylic	332:338	arg1	poly					327:330	poly	327:330	poly(acrylic acid) (PAA)	327:350	To eliminate the undesired swelling/shrinkage in the responding process, poly(acrylic acid) (PAA) was grafted to acryloyl chloride (AC)-modified bacterial cellulose (BC) by free-radical polymerization.
32100808	2	42	theme	acrylic	332:338	arg1	acid					340:343	acrylic acid	332:343	acrylic acid	332:343	To eliminate the undesired swelling/shrinkage in the responding process, poly(acrylic acid) (PAA) was grafted to acryloyl chloride (AC)-modified bacterial cellulose (BC) by free-radical polymerization.
32100808	1	43	theme	biomechanical	232:244	arg1	fields					246:251	biomechanical fields	232:251	biomechanical fields	232:251	Mechanically adaptive hydrogels can change their mechanical characteristics in response to external stimuli and have potential applications in biomechanical fields.
32100808	0	44	theme	adjustable	37:46	arg1	hydrogels					78:86	stiffness adjustable bacterial cellulose composite hydrogels	27:86	stiffness adjustable bacterial cellulose composite hydrogels	27:86	Osmotic squat actuation in stiffness adjustable bacterial cellulose composite hydrogels.
32100808	8	45	theme	more	1594:1597	arg1	options					1599:1605	more options	1594:1605	more options	1594:1605	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability are expected to provide more options in developing novel bioimplants and smart structures.
32100808	8	46	theme	composite	1459:1467	arg1	hydrogels					1469:1477	The developed pH-responsive mechanically adaptive BC composite hydrogels	1406:1477	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability	1406:1568	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability are expected to provide more options in developing novel bioimplants and smart structures.
32100808	2	47	theme	undesired	271:279	arg1	swelling/shrinkage					281:298	the undesired swelling/shrinkage	267:298	the undesired swelling/shrinkage in the responding process	267:324	To eliminate the undesired swelling/shrinkage in the responding process, poly(acrylic acid) (PAA) was grafted to acryloyl chloride (AC)-modified bacterial cellulose (BC) by free-radical polymerization.
32100808	7	48	from	softening	1345:1353	arg1	hydrogels					1382:1390	BC-g-PAA hydrogels	1373:1390	BC-g-PAA hydrogels	1373:1390	By virtue of the selective UV irradiation, spatiotemporally controllable softening with actuation in BC-g-PAA hydrogels was realized.
32100808	0	49	theme	stiffness	27:35	arg1	hydrogels					78:86	stiffness adjustable bacterial cellulose composite hydrogels	27:86	stiffness adjustable bacterial cellulose composite hydrogels	27:86	Osmotic squat actuation in stiffness adjustable bacterial cellulose composite hydrogels.
32100808	3	50	theme	MPa	715:717	arg1	stress					701:706	a stress	699:706	a stress of 0.1 MPa	699:717	The obtained BC-g-PAA composite hydrogels showed adjustable stiffness in compression, remained soft at pH lower than 6 (compression strain over 49% at a stress of 0.1 MPa), and stiffened when pH reached 7 (compression strain lower than 27% at a stress of 0.1 MPa), while the volume change ratio was consistently lower than 15%.
32100808	1	51	contain	have	201:204	arg1	hydrogels					111:119	Mechanically adaptive hydrogels	89:119	Mechanically adaptive hydrogels	89:119	Mechanically adaptive hydrogels can change their mechanical characteristics in response to external stimuli and have potential applications in biomechanical fields.
32100808	1	51	contain	have	201:204	arg2	applications					216:227	potential applications	206:227	potential applications	206:227	Mechanically adaptive hydrogels can change their mechanical characteristics in response to external stimuli and have potential applications in biomechanical fields.
32100808	3	52	theme	obtained	460:467	arg1	hydrogels					488:496	The obtained BC-g-PAA composite hydrogels	456:496	The obtained BC-g-PAA composite hydrogels	456:496	The obtained BC-g-PAA composite hydrogels showed adjustable stiffness in compression, remained soft at pH lower than 6 (compression strain over 49% at a stress of 0.1 MPa), and stiffened when pH reached 7 (compression strain lower than 27% at a stress of 0.1 MPa), while the volume change ratio was consistently lower than 15%.
32100808	0	53	theme	cellulose	58:66	arg1	hydrogels					78:86	stiffness adjustable bacterial cellulose composite hydrogels	27:86	stiffness adjustable bacterial cellulose composite hydrogels	27:86	Osmotic squat actuation in stiffness adjustable bacterial cellulose composite hydrogels.
32100808	5	54	theme	stable	1076:1081	arg1	hydrogels					1109:1117	fabricating volumetrically stable and mechanically adaptive hydrogels	1049:1117	fabricating volumetrically stable and mechanically adaptive hydrogels	1049:1117	The BC composite hydrogels exhibited dual pH-responsiveness after grafting PAA with poly[2-(dimethylamino)ethyl methacrylate], confirming the general availability of this strategy in fabricating volumetrically stable and mechanically adaptive hydrogels.
32100808	3	55	theme	MPa	623:625	arg1	stress					609:614	a stress	607:614	a stress of 0.1 MPa	607:625	The obtained BC-g-PAA composite hydrogels showed adjustable stiffness in compression, remained soft at pH lower than 6 (compression strain over 49% at a stress of 0.1 MPa), and stiffened when pH reached 7 (compression strain lower than 27% at a stress of 0.1 MPa), while the volume change ratio was consistently lower than 15%.
32100808	6	56	theme	hydrogels	1179:1187	arg1	softening					1157:1165	The surrounding solution-independent softening	1120:1165	The surrounding solution-independent softening of BC-g-PAA hydrogels	1120:1187	The surrounding solution-independent softening of BC-g-PAA hydrogels was observed in 8 min under UV irradiation via a photo-triggered pH jump reaction.
32100808	8	57	theme	high	1484:1487	arg1	stability					1501:1509	high dimensional stability	1484:1509	high dimensional stability	1484:1509	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability are expected to provide more options in developing novel bioimplants and smart structures.
32100808	3	58	theme	adjustable	505:514	arg1	stiffness					516:524	adjustable stiffness	505:524	adjustable stiffness	505:524	The obtained BC-g-PAA composite hydrogels showed adjustable stiffness in compression, remained soft at pH lower than 6 (compression strain over 49% at a stress of 0.1 MPa), and stiffened when pH reached 7 (compression strain lower than 27% at a stress of 0.1 MPa), while the volume change ratio was consistently lower than 15%.
32100808	0	59	theme	bacterial	48:56	arg1	hydrogels					78:86	stiffness adjustable bacterial cellulose composite hydrogels	27:86	stiffness adjustable bacterial cellulose composite hydrogels	27:86	Osmotic squat actuation in stiffness adjustable bacterial cellulose composite hydrogels.
32100808	6	60	theme	jump	1257:1260	arg1	reaction					1262:1269	a photo-triggered pH jump reaction	1236:1269	a photo-triggered pH jump reaction	1236:1269	The surrounding solution-independent softening of BC-g-PAA hydrogels was observed in 8 min under UV irradiation via a photo-triggered pH jump reaction.
32100808	8	61	theme	BC	1456:1457	arg1	hydrogels					1469:1477	The developed pH-responsive mechanically adaptive BC composite hydrogels	1406:1477	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability	1406:1568	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability are expected to provide more options in developing novel bioimplants and smart structures.
32100808	5	62	theme	poly[2-	950:956	arg1	methacrylate					978:989	poly[2-(dimethylamino)ethyl methacrylate	950:989	poly[2-(dimethylamino)ethyl methacrylate	950:989	The BC composite hydrogels exhibited dual pH-responsiveness after grafting PAA with poly[2-(dimethylamino)ethyl methacrylate], confirming the general availability of this strategy in fabricating volumetrically stable and mechanically adaptive hydrogels.
32100808	1	63	theme	mechanical	138:147	arg1	characteristics					149:163	their mechanical characteristics	132:163	their mechanical characteristics in response to external stimuli	132:195	Mechanically adaptive hydrogels can change their mechanical characteristics in response to external stimuli and have potential applications in biomechanical fields.
32100808	8	64	theme	adaptive	1447:1454	arg1	hydrogels					1469:1477	The developed pH-responsive mechanically adaptive BC composite hydrogels	1406:1477	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability	1406:1568	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability are expected to provide more options in developing novel bioimplants and smart structures.
32100808	7	65	theme	selective	1289:1297	arg1	irradiation					1302:1312	the selective UV irradiation	1285:1312	the selective UV irradiation	1285:1312	By virtue of the selective UV irradiation, spatiotemporally controllable softening with actuation in BC-g-PAA hydrogels was realized.
32100808	6	66	theme	pH	1254:1255	arg1	reaction					1262:1269	a photo-triggered pH jump reaction	1236:1269	a photo-triggered pH jump reaction	1236:1269	The surrounding solution-independent softening of BC-g-PAA hydrogels was observed in 8 min under UV irradiation via a photo-triggered pH jump reaction.
32100808	5	67	theme	dimethylamino	958:970	arg1	methacrylate					978:989	poly[2-(dimethylamino)ethyl methacrylate	950:989	poly[2-(dimethylamino)ethyl methacrylate	950:989	The BC composite hydrogels exhibited dual pH-responsiveness after grafting PAA with poly[2-(dimethylamino)ethyl methacrylate], confirming the general availability of this strategy in fabricating volumetrically stable and mechanically adaptive hydrogels.
32100808	2	68	theme	responding	307:316	arg1	process					318:324	the responding process	303:324	the responding process	303:324	To eliminate the undesired swelling/shrinkage in the responding process, poly(acrylic acid) (PAA) was grafted to acryloyl chloride (AC)-modified bacterial cellulose (BC) by free-radical polymerization.
32100808	3	69	dep	pH	559:560	arg1	strain					588:593	compression strain	576:593	compression strain over 49% at a stress of 0.1 MPa	576:625	The obtained BC-g-PAA composite hydrogels showed adjustable stiffness in compression, remained soft at pH lower than 6 (compression strain over 49% at a stress of 0.1 MPa), and stiffened when pH reached 7 (compression strain lower than 27% at a stress of 0.1 MPa), while the volume change ratio was consistently lower than 15%.
32100808	6	70	theme	photo-triggered	1238:1252	arg1	reaction					1262:1269	a photo-triggered pH jump reaction	1236:1269	a photo-triggered pH jump reaction	1236:1269	The surrounding solution-independent softening of BC-g-PAA hydrogels was observed in 8 min under UV irradiation via a photo-triggered pH jump reaction.
32100808	5	71	theme	dual	903:906	arg1	pH-responsiveness					908:924	dual pH-responsiveness	903:924	dual pH-responsiveness	903:924	The BC composite hydrogels exhibited dual pH-responsiveness after grafting PAA with poly[2-(dimethylamino)ethyl methacrylate], confirming the general availability of this strategy in fabricating volumetrically stable and mechanically adaptive hydrogels.
32100808	7	72	theme	controllable	1332:1343	arg1	softening					1345:1353	spatiotemporally controllable softening	1315:1353	spatiotemporally controllable softening with actuation in BC-g-PAA hydrogels	1315:1390	By virtue of the selective UV irradiation, spatiotemporally controllable softening with actuation in BC-g-PAA hydrogels was realized.
32100808	3	73	theme	volume	731:736	arg1	ratio					745:749	the volume change ratio	727:749	the volume change ratio	727:749	The obtained BC-g-PAA composite hydrogels showed adjustable stiffness in compression, remained soft at pH lower than 6 (compression strain over 49% at a stress of 0.1 MPa), and stiffened when pH reached 7 (compression strain lower than 27% at a stress of 0.1 MPa), while the volume change ratio was consistently lower than 15%.
32100808	3	73	theme	volume	731:736	arg1	lower					768:772	lower	768:772	lower	768:772	The obtained BC-g-PAA composite hydrogels showed adjustable stiffness in compression, remained soft at pH lower than 6 (compression strain over 49% at a stress of 0.1 MPa), and stiffened when pH reached 7 (compression strain lower than 27% at a stress of 0.1 MPa), while the volume change ratio was consistently lower than 15%.
32100808	8	74	theme	mechanically	1434:1445	arg1	hydrogels					1469:1477	The developed pH-responsive mechanically adaptive BC composite hydrogels	1406:1477	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability	1406:1568	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability are expected to provide more options in developing novel bioimplants and smart structures.
32100808	5	75	theme	ethyl	972:976	arg1	methacrylate					978:989	poly[2-(dimethylamino)ethyl methacrylate	950:989	poly[2-(dimethylamino)ethyl methacrylate	950:989	The BC composite hydrogels exhibited dual pH-responsiveness after grafting PAA with poly[2-(dimethylamino)ethyl methacrylate], confirming the general availability of this strategy in fabricating volumetrically stable and mechanically adaptive hydrogels.
32100808	6	76	theme	solution-independent	1136:1155	arg1	softening					1157:1165	The surrounding solution-independent softening	1120:1165	The surrounding solution-independent softening of BC-g-PAA hydrogels	1120:1187	The surrounding solution-independent softening of BC-g-PAA hydrogels was observed in 8 min under UV irradiation via a photo-triggered pH jump reaction.
32100808	8	77	with	hydrogels	1469:1477	arg1	stability					1501:1509	high dimensional stability	1484:1509	high dimensional stability	1484:1509	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability are expected to provide more options in developing novel bioimplants and smart structures.
32100808	8	77	with	hydrogels	1469:1477	arg1	squat					1543:1547	UV-activated spatiotemporal squat	1515:1547	UV-activated spatiotemporal squat actuating capability	1515:1568	The developed pH-responsive mechanically adaptive BC composite hydrogels with high dimensional stability and UV-activated spatiotemporal squat actuating capability are expected to provide more options in developing novel bioimplants and smart structures.
32100808	0	78	theme	composite	68:76	arg1	hydrogels					78:86	stiffness adjustable bacterial cellulose composite hydrogels	27:86	stiffness adjustable bacterial cellulose composite hydrogels	27:86	Osmotic squat actuation in stiffness adjustable bacterial cellulose composite hydrogels.
32100808	7	79	theme	UV	1299:1300	arg1	irradiation					1302:1312	the selective UV irradiation	1285:1312	the selective UV irradiation	1285:1312	By virtue of the selective UV irradiation, spatiotemporally controllable softening with actuation in BC-g-PAA hydrogels was realized.
32100808	6	80	theme	surrounding	1124:1134	arg1	softening					1157:1165	The surrounding solution-independent softening	1120:1165	The surrounding solution-independent softening of BC-g-PAA hydrogels	1120:1187	The surrounding solution-independent softening of BC-g-PAA hydrogels was observed in 8 min under UV irradiation via a photo-triggered pH jump reaction.
32100808	5	81	from	availability	1016:1027	arg1	hydrogels					1109:1117	fabricating volumetrically stable and mechanically adaptive hydrogels	1049:1117	fabricating volumetrically stable and mechanically adaptive hydrogels	1049:1117	The BC composite hydrogels exhibited dual pH-responsiveness after grafting PAA with poly[2-(dimethylamino)ethyl methacrylate], confirming the general availability of this strategy in fabricating volumetrically stable and mechanically adaptive hydrogels.
32100808	6	82	located	observed	1193:1200	arg1	min					1207:1209	8 min	1205:1209	8 min	1205:1209	The surrounding solution-independent softening of BC-g-PAA hydrogels was observed in 8 min under UV irradiation via a photo-triggered pH jump reaction.
32100808	6	82	located	observed	1193:1200	arg2	softening					1157:1165	The surrounding solution-independent softening	1120:1165	The surrounding solution-independent softening of BC-g-PAA hydrogels	1120:1187	The surrounding solution-independent softening of BC-g-PAA hydrogels was observed in 8 min under UV irradiation via a photo-triggered pH jump reaction.
32538381	7	0	theme	glucose	1272:1278	arg1	level					1280:1284	a blood glucose level	1264:1284	a blood glucose level	1264:1284	Furthermore, the bio-properties of the insulin were retained and it showed a blood glucose level reducing effect for more than 60 hours after injection to a streptozotocin (STZ)-induced diabetic mouse model.
32538381	2	1	theme	ethylene	372:379	arg1	-b-poly					364:370	an oligomer serine-b-poly(lactide)-b-poly	330:370	an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer	330:462	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	2	1	theme	ethylene	372:379	arg1	glycol					381:386	ethylene glycol	372:386	ethylene glycol	372:386	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	2	2	theme	electrosprayed	496:509	arg1	CIN					524:526	CIN	524:526	CIN	524:526	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	2	2	theme	electrosprayed	496:509	arg1	nanospheres					511:521	matrix and chitosan-insulin electrosprayed nanospheres	468:521	nanospheres	511:521	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	6	3	theme	steady-state	1169:1180	arg1	level					1182:1186	a steady-state level	1167:1186	a steady-state level	1167:1186	The result showed that insulin concentrations in blood plasma were maintained at a steady-state level.
32538381	0	4	contain	containing	53:62	arg2	chitosan-insulin					64:79	chitosan-insulin	64:79	chitosan-insulin	64:79	A novel injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composite for an insulin delivery system in type I diabetes treatment.
32538381	0	4	contain	containing	53:62	arg1	hydrogel					44:51	A novel injectable pH-temperature sensitive hydrogel	0:51	A novel injectable pH-temperature sensitive hydrogel containing chitosan-insulin	0:79	A novel injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composite for an insulin delivery system in type I diabetes treatment.
32538381	1	5	theme	novel	180:184	arg1	system					213:218	A novel insulin composite delivery system	178:218	A novel insulin composite delivery system	178:218	A novel insulin composite delivery system was prepared and characterized.
32538381	8	6	theme	nanosphere	1517:1526	arg1	composites					1528:1537	chitosan-insulin electrosprayed nanosphere composites	1485:1537	chitosan-insulin electrosprayed nanosphere composites	1485:1537	The results suggested that this injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composites has promising potential applications for type 1 diabetes treatment.
32538381	6	7	theme	insulin	1109:1115	arg1	concentrations					1117:1130	insulin concentrations	1109:1130	insulin concentrations in blood plasma	1109:1146	The result showed that insulin concentrations in blood plasma were maintained at a steady-state level.
32538381	0	8	theme	insulin	124:130	arg1	system					141:146	an insulin delivery system	121:146	an insulin delivery system in type I diabetes treatment	121:175	A novel injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composite for an insulin delivery system in type I diabetes treatment.
32538381	7	9	theme	insulin	1228:1234	arg1	bio-properties					1206:1219	the bio-properties	1202:1219	the bio-properties of the insulin	1202:1234	Furthermore, the bio-properties of the insulin were retained and it showed a blood glucose level reducing effect for more than 60 hours after injection to a streptozotocin (STZ)-induced diabetic mouse model.
32538381	8	10	theme	type	1580:1583	arg1	diabetes					1587:1594	type 1 diabetes	1580:1594	type 1 diabetes treatment	1580:1604	The results suggested that this injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composites has promising potential applications for type 1 diabetes treatment.
32538381	8	11	theme	sensitive	1455:1463	arg1	hydrogel					1465:1472	this injectable pH-temperature sensitive hydrogel	1424:1472	this injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composites	1424:1537	The results suggested that this injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composites has promising potential applications for type 1 diabetes treatment.
32538381	4	12	theme	degradation	816:826	arg1	time					828:831	the degradation time	812:831	the degradation time of the hydrogel depot	812:853	The chitosan-insulin nanospheres uniformly distributed in the matrix had a reinforcing effect on the mechanical properties and prolonged the degradation time of the hydrogel depot under body conditions.
32538381	2	13	theme	constituent	532:542	arg1	materials					544:552	constituent materials	532:552	constituent materials	532:552	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	8	14	theme	pH-temperature	1440:1453	arg1	hydrogel					1465:1472	this injectable pH-temperature sensitive hydrogel	1424:1472	this injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composites	1424:1537	The results suggested that this injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composites has promising potential applications for type 1 diabetes treatment.
32538381	5	15	theme	induced	1011:1017	arg1	mice					1035:1038	induced diabetic BALB/c mice	1011:1038	induced diabetic BALB/c mice	1011:1038	The composite solutions accommodating different concentrations of the chitosan-insulin nanospheres were subcutaneously injected into induced diabetic BALB/c mice to study the in vivo insulin-release profile.
32538381	5	16	theme	chitosan-insulin	948:963	arg1	nanospheres					965:975	the chitosan-insulin nanospheres	944:975	the chitosan-insulin nanospheres	944:975	The composite solutions accommodating different concentrations of the chitosan-insulin nanospheres were subcutaneously injected into induced diabetic BALB/c mice to study the in vivo insulin-release profile.
32538381	5	17	theme	BALB/c	1028:1033	arg1	mice					1035:1038	induced diabetic BALB/c mice	1011:1038	induced diabetic BALB/c mice	1011:1038	The composite solutions accommodating different concentrations of the chitosan-insulin nanospheres were subcutaneously injected into induced diabetic BALB/c mice to study the in vivo insulin-release profile.
32538381	5	18	theme	nanospheres	965:975	arg1	concentrations					926:939	different concentrations	916:939	different concentrations of the chitosan-insulin nanospheres	916:975	The composite solutions accommodating different concentrations of the chitosan-insulin nanospheres were subcutaneously injected into induced diabetic BALB/c mice to study the in vivo insulin-release profile.
32538381	4	19	theme	depot	849:853	arg1	time					828:831	the degradation time	812:831	the degradation time of the hydrogel depot	812:853	The chitosan-insulin nanospheres uniformly distributed in the matrix had a reinforcing effect on the mechanical properties and prolonged the degradation time of the hydrogel depot under body conditions.
32538381	1	20	theme	insulin	186:192	arg1	system					213:218	A novel insulin composite delivery system	178:218	A novel insulin composite delivery system	178:218	A novel insulin composite delivery system was prepared and characterized.
32538381	0	21	theme	delivery	132:139	arg1	system					141:146	an insulin delivery system	121:146	an insulin delivery system in type I diabetes treatment	121:175	A novel injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composite for an insulin delivery system in type I diabetes treatment.
32538381	5	22	theme	diabetic	1019:1026	arg1	mice					1035:1038	induced diabetic BALB/c mice	1011:1038	induced diabetic BALB/c mice	1011:1038	The composite solutions accommodating different concentrations of the chitosan-insulin nanospheres were subcutaneously injected into induced diabetic BALB/c mice to study the in vivo insulin-release profile.
32538381	2	23	theme	temperature-sensitive	289:309	arg1	-b-poly					364:370	an oligomer serine-b-poly(lactide)-b-poly	330:370	an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer	330:462	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	2	23	theme	temperature-sensitive	289:309	arg1	hydrogel					311:318	a pH- and temperature-sensitive hydrogel	279:318	a pH- and temperature-sensitive hydrogel	279:318	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	8	24	theme	promising	1543:1551	arg1	applications					1563:1574	promising potential applications	1543:1574	promising potential applications for type 1 diabetes treatment	1543:1604	The results suggested that this injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composites has promising potential applications for type 1 diabetes treatment.
32538381	2	25	theme	lactide	396:402	arg1	copolymer					454:462	-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer	388:462	an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer	330:462	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	0	26	theme	novel	2:6	arg1	hydrogel					44:51	A novel injectable pH-temperature sensitive hydrogel	0:51	A novel injectable pH-temperature sensitive hydrogel containing chitosan-insulin	0:79	A novel injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composite for an insulin delivery system in type I diabetes treatment.
32538381	6	27	from	concentrations	1117:1130	arg1	plasma					1141:1146	blood plasma	1135:1146	blood plasma	1135:1146	The result showed that insulin concentrations in blood plasma were maintained at a steady-state level.
32538381	2	28	theme	serine-b-poly	342:354	arg1	-b-poly					364:370	an oligomer serine-b-poly(lactide)-b-poly	330:370	an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer	330:462	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	2	28	theme	serine-b-poly	342:354	arg1	glycol					381:386	ethylene glycol	372:386	ethylene glycol	372:386	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	2	28	theme	serine-b-poly	342:354	arg1	hydrogel					311:318	a pH- and temperature-sensitive hydrogel	279:318	a pH- and temperature-sensitive hydrogel	279:318	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	1	29	theme	delivery	204:211	arg1	system					213:218	A novel insulin composite delivery system	178:218	A novel insulin composite delivery system	178:218	A novel insulin composite delivery system was prepared and characterized.
32538381	5	30	theme	composite	882:890	arg1	solutions					892:900	The composite solutions	878:900	The composite solutions accommodating different concentrations of the chitosan-insulin nanospheres	878:975	The composite solutions accommodating different concentrations of the chitosan-insulin nanospheres were subcutaneously injected into induced diabetic BALB/c mice to study the in vivo insulin-release profile.
32538381	2	31	theme	oligomer	333:340	arg1	-b-poly					364:370	an oligomer serine-b-poly(lactide)-b-poly	330:370	an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer	330:462	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	2	31	theme	oligomer	333:340	arg1	glycol					381:386	ethylene glycol	372:386	ethylene glycol	372:386	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	2	31	theme	oligomer	333:340	arg1	hydrogel					311:318	a pH- and temperature-sensitive hydrogel	279:318	a pH- and temperature-sensitive hydrogel	279:318	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	2	32	theme	-b-poly	388:394	arg1	copolymer					454:462	-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer	388:462	an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer	330:462	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	0	33	theme	pH-temperature	19:32	arg1	hydrogel					44:51	A novel injectable pH-temperature sensitive hydrogel	0:51	A novel injectable pH-temperature sensitive hydrogel containing chitosan-insulin	0:79	A novel injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composite for an insulin delivery system in type I diabetes treatment.
32538381	0	34	theme	type	151:154	arg1	diabetes					158:165	type I diabetes	151:165	type I diabetes treatment	151:175	A novel injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composite for an insulin delivery system in type I diabetes treatment.
32538381	2	35	dep	-b-poly	364:370	arg1	copolymer					454:462	-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer	388:462	an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer	330:462	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	5	36	theme	insulin-release	1061:1075	arg1	profile					1077:1083	the in vivo insulin-release profile	1049:1083	the in vivo insulin-release profile	1049:1083	The composite solutions accommodating different concentrations of the chitosan-insulin nanospheres were subcutaneously injected into induced diabetic BALB/c mice to study the in vivo insulin-release profile.
32538381	2	37	theme	OS-PLA-PEG-PLA-OS	424:440	arg1	copolymer					454:462	-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer	388:462	an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer	330:462	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	0	38	theme	injectable	8:17	arg1	hydrogel					44:51	A novel injectable pH-temperature sensitive hydrogel	0:51	A novel injectable pH-temperature sensitive hydrogel containing chitosan-insulin	0:79	A novel injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composite for an insulin delivery system in type I diabetes treatment.
32538381	5	39	theme	in	1053:1054	arg1	profile					1077:1083	the in vivo insulin-release profile	1049:1083	the in vivo insulin-release profile	1049:1083	The composite solutions accommodating different concentrations of the chitosan-insulin nanospheres were subcutaneously injected into induced diabetic BALB/c mice to study the in vivo insulin-release profile.
32538381	3	40	theme	OS-PLA-PEG-PLA-OS	577:593	arg1	copolymer					606:614	the OS-PLA-PEG-PLA-OS pentablock copolymer	573:614	the OS-PLA-PEG-PLA-OS pentablock copolymer	573:614	The properties of the OS-PLA-PEG-PLA-OS pentablock copolymer and the chitosan-insulin nanoparticles were characterized.
32538381	4	41	theme	chitosan-insulin	679:694	arg1	nanospheres					696:706	The chitosan-insulin nanospheres	675:706	The chitosan-insulin nanospheres uniformly distributed in the matrix	675:742	The chitosan-insulin nanospheres uniformly distributed in the matrix had a reinforcing effect on the mechanical properties and prolonged the degradation time of the hydrogel depot under body conditions.
32538381	2	42	theme	chitosan-insulin	479:494	arg1	CIN					524:526	CIN	524:526	CIN	524:526	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	2	42	theme	chitosan-insulin	479:494	arg1	nanospheres					511:521	matrix and chitosan-insulin electrosprayed nanospheres	468:521	nanospheres	511:521	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	0	43	theme	diabetes	158:165	arg1	treatment					167:175	type I diabetes treatment	151:175	type I diabetes treatment	151:175	A novel injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composite for an insulin delivery system in type I diabetes treatment.
32538381	3	44	theme	chitosan-insulin	624:639	arg1	nanoparticles					641:653	the chitosan-insulin nanoparticles	620:653	the chitosan-insulin nanoparticles	620:653	The properties of the OS-PLA-PEG-PLA-OS pentablock copolymer and the chitosan-insulin nanoparticles were characterized.
32538381	2	45	theme	serine	416:421	arg1	copolymer					454:462	-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer	388:462	an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer	330:462	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	0	46	theme	sensitive	34:42	arg1	hydrogel					44:51	A novel injectable pH-temperature sensitive hydrogel	0:51	A novel injectable pH-temperature sensitive hydrogel containing chitosan-insulin	0:79	A novel injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composite for an insulin delivery system in type I diabetes treatment.
32538381	7	47	theme	blood	1266:1270	arg1	level					1280:1284	a blood glucose level	1264:1284	a blood glucose level	1264:1284	Furthermore, the bio-properties of the insulin were retained and it showed a blood glucose level reducing effect for more than 60 hours after injection to a streptozotocin (STZ)-induced diabetic mouse model.
32538381	2	48	theme	pentablock	443:452	arg1	copolymer					454:462	-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer	388:462	an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer	330:462	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	2	49	theme	-b-oligomer	404:414	arg1	copolymer					454:462	-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer	388:462	an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer	330:462	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	2	50	theme	matrix	468:473	arg1	CIN					524:526	CIN	524:526	CIN	524:526	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	2	50	theme	matrix	468:473	arg1	nanospheres					511:521	matrix and chitosan-insulin electrosprayed nanospheres	468:521	nanospheres	511:521	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	8	51	theme	electrosprayed	1502:1515	arg1	composites					1528:1537	chitosan-insulin electrosprayed nanosphere composites	1485:1537	chitosan-insulin electrosprayed nanosphere composites	1485:1537	The results suggested that this injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composites has promising potential applications for type 1 diabetes treatment.
32538381	8	52	theme	chitosan-insulin	1485:1500	arg1	composites					1528:1537	chitosan-insulin electrosprayed nanosphere composites	1485:1537	chitosan-insulin electrosprayed nanosphere composites	1485:1537	The results suggested that this injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composites has promising potential applications for type 1 diabetes treatment.
32538381	4	53	theme	mechanical	776:785	arg1	properties					787:796	the mechanical properties	772:796	the mechanical properties	772:796	The chitosan-insulin nanospheres uniformly distributed in the matrix had a reinforcing effect on the mechanical properties and prolonged the degradation time of the hydrogel depot under body conditions.
32538381	5	54	dep	in	1053:1054	arg1	vivo					1056:1059	vivo	1056:1059	vivo	1056:1059	The composite solutions accommodating different concentrations of the chitosan-insulin nanospheres were subcutaneously injected into induced diabetic BALB/c mice to study the in vivo insulin-release profile.
32538381	0	55	from	system	141:146	arg1	treatment					167:175	type I diabetes treatment	151:175	type I diabetes treatment	151:175	A novel injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composite for an insulin delivery system in type I diabetes treatment.
32538381	8	56	theme	potential	1553:1561	arg1	applications					1563:1574	promising potential applications	1543:1574	promising potential applications for type 1 diabetes treatment	1543:1604	The results suggested that this injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composites has promising potential applications for type 1 diabetes treatment.
32538381	7	57	theme	diabetic	1375:1382	arg1	model					1390:1394	a streptozotocin (STZ)-induced diabetic mouse model	1344:1394	a streptozotocin (STZ)-induced diabetic mouse model	1344:1394	Furthermore, the bio-properties of the insulin were retained and it showed a blood glucose level reducing effect for more than 60 hours after injection to a streptozotocin (STZ)-induced diabetic mouse model.
32538381	7	58	theme	mouse	1384:1388	arg1	model					1390:1394	a streptozotocin (STZ)-induced diabetic mouse model	1344:1394	a streptozotocin (STZ)-induced diabetic mouse model	1344:1394	Furthermore, the bio-properties of the insulin were retained and it showed a blood glucose level reducing effect for more than 60 hours after injection to a streptozotocin (STZ)-induced diabetic mouse model.
32538381	3	59	theme	pentablock	595:604	arg1	copolymer					606:614	the OS-PLA-PEG-PLA-OS pentablock copolymer	573:614	the OS-PLA-PEG-PLA-OS pentablock copolymer	573:614	The properties of the OS-PLA-PEG-PLA-OS pentablock copolymer and the chitosan-insulin nanoparticles were characterized.
32538381	4	60	contain	had	744:746	arg1	nanospheres					696:706	The chitosan-insulin nanospheres	675:706	The chitosan-insulin nanospheres uniformly distributed in the matrix	675:742	The chitosan-insulin nanospheres uniformly distributed in the matrix had a reinforcing effect on the mechanical properties and prolonged the degradation time of the hydrogel depot under body conditions.
32538381	4	60	contain	had	744:746	arg2	effect					762:767	a reinforcing effect	748:767	a reinforcing effect	748:767	The chitosan-insulin nanospheres uniformly distributed in the matrix had a reinforcing effect on the mechanical properties and prolonged the degradation time of the hydrogel depot under body conditions.
32538381	4	61	theme	body	861:864	arg1	conditions					866:875	body conditions	861:875	body conditions	861:875	The chitosan-insulin nanospheres uniformly distributed in the matrix had a reinforcing effect on the mechanical properties and prolonged the degradation time of the hydrogel depot under body conditions.
32538381	3	62	theme	nanoparticles	641:653	arg1	properties					559:568	The properties	555:568	The properties of the OS-PLA-PEG-PLA-OS pentablock copolymer and the chitosan-insulin nanoparticles	555:653	The properties of the OS-PLA-PEG-PLA-OS pentablock copolymer and the chitosan-insulin nanoparticles were characterized.
32538381	2	63	theme	lactide	356:362	arg1	-b-poly					364:370	an oligomer serine-b-poly(lactide)-b-poly	330:370	an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer	330:462	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	2	63	theme	lactide	356:362	arg1	glycol					381:386	ethylene glycol	372:386	ethylene glycol	372:386	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	2	63	theme	lactide	356:362	arg1	hydrogel					311:318	a pH- and temperature-sensitive hydrogel	279:318	a pH- and temperature-sensitive hydrogel	279:318	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	4	64	theme	hydrogel	840:847	arg1	depot					849:853	the hydrogel depot	836:853	the hydrogel depot	836:853	The chitosan-insulin nanospheres uniformly distributed in the matrix had a reinforcing effect on the mechanical properties and prolonged the degradation time of the hydrogel depot under body conditions.
32538381	2	65	theme	pH-	281:283	arg1	-b-poly					364:370	an oligomer serine-b-poly(lactide)-b-poly	330:370	an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer	330:462	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	2	65	theme	pH-	281:283	arg1	hydrogel					311:318	a pH- and temperature-sensitive hydrogel	279:318	a pH- and temperature-sensitive hydrogel	279:318	The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials.
32538381	7	66	theme	-induced	1366:1373	arg1	model					1390:1394	a streptozotocin (STZ)-induced diabetic mouse model	1344:1394	a streptozotocin (STZ)-induced diabetic mouse model	1344:1394	Furthermore, the bio-properties of the insulin were retained and it showed a blood glucose level reducing effect for more than 60 hours after injection to a streptozotocin (STZ)-induced diabetic mouse model.
32538381	8	67	theme	injectable	1429:1438	arg1	hydrogel					1465:1472	this injectable pH-temperature sensitive hydrogel	1424:1472	this injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composites	1424:1537	The results suggested that this injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composites has promising potential applications for type 1 diabetes treatment.
32538381	0	68	theme	nanosphere	96:105	arg1	composite					107:115	nanosphere composite	96:115	nanosphere composite	96:115	A novel injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composite for an insulin delivery system in type I diabetes treatment.
32538381	8	69	theme	diabetes	1587:1594	arg1	treatment					1596:1604	type 1 diabetes treatment	1580:1604	type 1 diabetes treatment	1580:1604	The results suggested that this injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composites has promising potential applications for type 1 diabetes treatment.
32538381	5	70	theme	different	916:924	arg1	concentrations					926:939	different concentrations	916:939	different concentrations of the chitosan-insulin nanospheres	916:975	The composite solutions accommodating different concentrations of the chitosan-insulin nanospheres were subcutaneously injected into induced diabetic BALB/c mice to study the in vivo insulin-release profile.
32538381	4	71	theme	reinforcing	750:760	arg1	effect					762:767	a reinforcing effect	748:767	a reinforcing effect	748:767	The chitosan-insulin nanospheres uniformly distributed in the matrix had a reinforcing effect on the mechanical properties and prolonged the degradation time of the hydrogel depot under body conditions.
32538381	1	72	theme	composite	194:202	arg1	system					213:218	A novel insulin composite delivery system	178:218	A novel insulin composite delivery system	178:218	A novel insulin composite delivery system was prepared and characterized.
32538381	6	73	theme	blood	1135:1139	arg1	plasma					1141:1146	blood plasma	1135:1146	blood plasma	1135:1146	The result showed that insulin concentrations in blood plasma were maintained at a steady-state level.
32538381	8	74	contain	has	1539:1541	arg1	hydrogel					1465:1472	this injectable pH-temperature sensitive hydrogel	1424:1472	this injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composites	1424:1537	The results suggested that this injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composites has promising potential applications for type 1 diabetes treatment.
32538381	8	74	contain	has	1539:1541	arg2	applications					1563:1574	promising potential applications	1543:1574	promising potential applications for type 1 diabetes treatment	1543:1604	The results suggested that this injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composites has promising potential applications for type 1 diabetes treatment.
32538381	3	75	theme	copolymer	606:614	arg1	properties					559:568	The properties	555:568	The properties of the OS-PLA-PEG-PLA-OS pentablock copolymer and the chitosan-insulin nanoparticles	555:653	The properties of the OS-PLA-PEG-PLA-OS pentablock copolymer and the chitosan-insulin nanoparticles were characterized.
32538381	8	76	contain	containing	1474:1483	arg1	hydrogel					1465:1472	this injectable pH-temperature sensitive hydrogel	1424:1472	this injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composites	1424:1537	The results suggested that this injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composites has promising potential applications for type 1 diabetes treatment.
32538381	8	76	contain	containing	1474:1483	arg2	composites					1528:1537	chitosan-insulin electrosprayed nanosphere composites	1485:1537	chitosan-insulin electrosprayed nanosphere composites	1485:1537	The results suggested that this injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composites has promising potential applications for type 1 diabetes treatment.
33858576	0	0	theme	crystal	80:86	arg1	morphology					88:97	the crystal morphology	76:97	the crystal morphology of ZnAl-LDHs	76:110	The photothermal stability of CNFs/ZnAl-LDHs composited films: Influence of the crystal morphology of ZnAl-LDHs.
33858576	6	1	from	application	1116:1126	arg1	fields					1135:1140	the fields	1131:1140	the fields of photonics and electronics	1131:1169	This work provided a practical and effective way for preparation of photothermal-stable CNFs-based transparent films for industrial application in the fields of photonics and electronics.
33858576	1	2	theme	groups	152:157	arg1	existence					117:125	The existence	113:125	The existence of hydroxyl and carboxyl groups	113:157	The existence of hydroxyl and carboxyl groups makes the photothermal stability of cellulose nanofibers (CNFs) poor and thus limits its scale application.
33858576	2	3	theme	LDHs	333:336	arg1	nanopowders					339:349	layered double hydroxides (LDHs) nanopowders	306:349	layered double hydroxides (LDHs) nanopowders	306:349	This problem could be solved by doping layered double hydroxides (LDHs) nanopowders with opposite charge on the surface of CNFs.
33858576	6	4	theme	industrial	1105:1114	arg1	application					1116:1126	industrial application	1105:1126	industrial application in the fields of photonics and electronics	1105:1169	This work provided a practical and effective way for preparation of photothermal-stable CNFs-based transparent films for industrial application in the fields of photonics and electronics.
33858576	3	5	dep	ZnAl-LDHs	532:540	arg1	i.e.					527:530	i.e.	527:530	i.e.	527:530	This work mainly focused on investigation of the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films.
33858576	3	6	theme	agent	520:524	arg1	morphology					470:479	the crystal morphology	458:479	the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs)	458:541	This work mainly focused on investigation of the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films.
33858576	5	7	theme	light	936:940	arg1	transmittance					942:954	any serious light transmittance	924:954	any serious light transmittance deteriorating after doping	924:981	Specially, the ZnAl-LDHs with uniform crystal morphology could be prepared by controlling the molar ratio of Zn/Al at 3:1 and thus enhance the photothermal stability of CNFs-based films without any serious light transmittance deteriorating after doping.
33858576	1	8	theme	scale	248:252	arg1	application					254:264	its scale application	244:264	its scale application	244:264	The existence of hydroxyl and carboxyl groups makes the photothermal stability of cellulose nanofibers (CNFs) poor and thus limits its scale application.
33858576	0	9	theme	morphology	88:97	arg1	Influence					63:71	Influence	63:71	films: Influence of the crystal morphology of ZnAl-LDHs	56:110	The photothermal stability of CNFs/ZnAl-LDHs composited films: Influence of the crystal morphology of ZnAl-LDHs.
33858576	3	10	theme	morphology	470:479	arg1	influence					445:453	the influence	441:453	the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films	441:601	This work mainly focused on investigation of the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films.
33858576	2	11	theme	layered	306:312	arg1	nanopowders					339:349	layered double hydroxides (LDHs) nanopowders	306:349	layered double hydroxides (LDHs) nanopowders	306:349	This problem could be solved by doping layered double hydroxides (LDHs) nanopowders with opposite charge on the surface of CNFs.
33858576	6	12	theme	photonics	1145:1153	arg1	fields					1135:1140	the fields	1131:1140	the fields of photonics and electronics	1131:1169	This work provided a practical and effective way for preparation of photothermal-stable CNFs-based transparent films for industrial application in the fields of photonics and electronics.
33858576	5	13	theme	molar	824:828	arg1	ratio					830:834	the molar ratio	820:834	the molar ratio of Zn/Al at 3:1	820:850	Specially, the ZnAl-LDHs with uniform crystal morphology could be prepared by controlling the molar ratio of Zn/Al at 3:1 and thus enhance the photothermal stability of CNFs-based films without any serious light transmittance deteriorating after doping.
33858576	3	14	theme	ultraviolet	498:508	arg1	agent					520:524	the inorganic ultraviolet shielding agent	484:524	the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs)	484:541	This work mainly focused on investigation of the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films.
33858576	3	14	theme	ultraviolet	498:508	arg1	ZnAl-LDHs					532:540	ZnAl-LDHs	532:540	ZnAl-LDHs	532:540	This work mainly focused on investigation of the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films.
33858576	5	15	theme	CNFs-based	899:908	arg1	films					910:914	CNFs-based films	899:914	CNFs-based films	899:914	Specially, the ZnAl-LDHs with uniform crystal morphology could be prepared by controlling the molar ratio of Zn/Al at 3:1 and thus enhance the photothermal stability of CNFs-based films without any serious light transmittance deteriorating after doping.
33858576	5	16	theme	Zn/Al	839:843	arg1	ratio					830:834	the molar ratio	820:834	the molar ratio of Zn/Al at 3:1	820:850	Specially, the ZnAl-LDHs with uniform crystal morphology could be prepared by controlling the molar ratio of Zn/Al at 3:1 and thus enhance the photothermal stability of CNFs-based films without any serious light transmittance deteriorating after doping.
33858576	0	17	theme	ZnAl-LDHs	102:110	arg1	morphology					88:97	the crystal morphology	76:97	the crystal morphology of ZnAl-LDHs	76:110	The photothermal stability of CNFs/ZnAl-LDHs composited films: Influence of the crystal morphology of ZnAl-LDHs.
33858576	3	18	from	influence	445:453	arg1	stability					558:566	the thermal stability	546:566	the thermal stability of CNFs/ZnAl-LDHs composited films	546:601	This work mainly focused on investigation of the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films.
33858576	3	19	theme	shielding	510:518	arg1	agent					520:524	the inorganic ultraviolet shielding agent	484:524	the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs)	484:541	This work mainly focused on investigation of the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films.
33858576	3	19	theme	shielding	510:518	arg1	ZnAl-LDHs					532:540	ZnAl-LDHs	532:540	ZnAl-LDHs	532:540	This work mainly focused on investigation of the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films.
33858576	1	20	theme	photothermal	169:180	arg1	stability					182:190	the photothermal stability	165:190	the photothermal stability of cellulose nanofibers (CNFs) poor	165:226	The existence of hydroxyl and carboxyl groups makes the photothermal stability of cellulose nanofibers (CNFs) poor and thus limits its scale application.
33858576	0	21	theme	photothermal	4:15	arg1	stability					17:25	The photothermal stability	0:25	The photothermal stability of CNFs/ZnAl-LDHs	0:43	The photothermal stability of CNFs/ZnAl-LDHs composited films: Influence of the crystal morphology of ZnAl-LDHs.
33858576	3	22	theme	inorganic	488:496	arg1	agent					520:524	the inorganic ultraviolet shielding agent	484:524	the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs)	484:541	This work mainly focused on investigation of the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films.
33858576	3	22	theme	inorganic	488:496	arg1	ZnAl-LDHs					532:540	ZnAl-LDHs	532:540	ZnAl-LDHs	532:540	This work mainly focused on investigation of the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films.
33858576	6	23	theme	films	1095:1099	arg1	preparation					1037:1047	preparation	1037:1047	preparation of photothermal-stable CNFs-based transparent films for industrial application in the fields of photonics and electronics	1037:1169	This work provided a practical and effective way for preparation of photothermal-stable CNFs-based transparent films for industrial application in the fields of photonics and electronics.
33858576	4	24	theme	films	723:727	arg1	stability					699:707	the photothermal stability	682:707	the photothermal stability of CNFs-based films	682:727	The results showed that the morphology of LDHs was positively correlated with the photothermal stability of CNFs-based films.
33858576	4	25	theme	LDHs	646:649	arg1	morphology					632:641	the morphology	628:641	the morphology of LDHs	628:649	The results showed that the morphology of LDHs was positively correlated with the photothermal stability of CNFs-based films.
33858576	4	26	theme	CNFs-based	712:721	arg1	films					723:727	CNFs-based films	712:727	CNFs-based films	712:727	The results showed that the morphology of LDHs was positively correlated with the photothermal stability of CNFs-based films.
33858576	2	27	theme	opposite	356:363	arg1	charge					365:370	opposite charge	356:370	opposite charge on the surface of CNFs	356:393	This problem could be solved by doping layered double hydroxides (LDHs) nanopowders with opposite charge on the surface of CNFs.
33858576	5	28	theme	films	910:914	arg1	stability					886:894	the photothermal stability	869:894	the photothermal stability of CNFs-based films	869:914	Specially, the ZnAl-LDHs with uniform crystal morphology could be prepared by controlling the molar ratio of Zn/Al at 3:1 and thus enhance the photothermal stability of CNFs-based films without any serious light transmittance deteriorating after doping.
33858576	5	29	theme	serious	928:934	arg1	transmittance					942:954	any serious light transmittance	924:954	any serious light transmittance deteriorating after doping	924:981	Specially, the ZnAl-LDHs with uniform crystal morphology could be prepared by controlling the molar ratio of Zn/Al at 3:1 and thus enhance the photothermal stability of CNFs-based films without any serious light transmittance deteriorating after doping.
33858576	1	30	theme	cellulose	195:203	arg1	stability					182:190	the photothermal stability	165:190	the photothermal stability of cellulose nanofibers (CNFs) poor	165:226	The existence of hydroxyl and carboxyl groups makes the photothermal stability of cellulose nanofibers (CNFs) poor and thus limits its scale application.
33858576	5	31	theme	photothermal	873:884	arg1	stability					886:894	the photothermal stability	869:894	the photothermal stability of CNFs-based films	869:914	Specially, the ZnAl-LDHs with uniform crystal morphology could be prepared by controlling the molar ratio of Zn/Al at 3:1 and thus enhance the photothermal stability of CNFs-based films without any serious light transmittance deteriorating after doping.
33858576	3	32	from	stability	558:566	arg1	investigation					424:436	investigation	424:436	investigation of the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films	424:601	This work mainly focused on investigation of the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films.
33858576	2	33	theme	CNFs	390:393	arg1	surface					379:385	the surface	375:385	the surface of CNFs	375:393	This problem could be solved by doping layered double hydroxides (LDHs) nanopowders with opposite charge on the surface of CNFs.
33858576	5	34	theme	uniform	760:766	arg1	morphology					776:785	uniform crystal morphology	760:785	uniform crystal morphology	760:785	Specially, the ZnAl-LDHs with uniform crystal morphology could be prepared by controlling the molar ratio of Zn/Al at 3:1 and thus enhance the photothermal stability of CNFs-based films without any serious light transmittance deteriorating after doping.
33858576	3	35	theme	crystal	462:468	arg1	morphology					470:479	the crystal morphology	458:479	the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs)	458:541	This work mainly focused on investigation of the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films.
33858576	6	36	theme	photothermal-stable	1052:1070	arg1	films					1095:1099	photothermal-stable CNFs-based transparent films	1052:1099	photothermal-stable CNFs-based transparent films for industrial application in the fields of photonics and electronics	1052:1169	This work provided a practical and effective way for preparation of photothermal-stable CNFs-based transparent films for industrial application in the fields of photonics and electronics.
33858576	0	37	theme	CNFs/ZnAl-LDHs	30:43	arg1	stability					17:25	The photothermal stability	0:25	The photothermal stability of CNFs/ZnAl-LDHs	0:43	The photothermal stability of CNFs/ZnAl-LDHs composited films: Influence of the crystal morphology of ZnAl-LDHs.
33858576	5	38	theme	crystal	768:774	arg1	morphology					776:785	uniform crystal morphology	760:785	uniform crystal morphology	760:785	Specially, the ZnAl-LDHs with uniform crystal morphology could be prepared by controlling the molar ratio of Zn/Al at 3:1 and thus enhance the photothermal stability of CNFs-based films without any serious light transmittance deteriorating after doping.
33858576	0	39	dep	films	56:60	arg1	Influence					63:71	Influence	63:71	films: Influence of the crystal morphology of ZnAl-LDHs	56:110	The photothermal stability of CNFs/ZnAl-LDHs composited films: Influence of the crystal morphology of ZnAl-LDHs.
33858576	2	40	from	charge	365:370	arg1	surface					379:385	the surface	375:385	the surface of CNFs	375:393	This problem could be solved by doping layered double hydroxides (LDHs) nanopowders with opposite charge on the surface of CNFs.
33858576	6	41	theme	CNFs-based	1072:1081	arg1	films					1095:1099	photothermal-stable CNFs-based transparent films	1052:1099	photothermal-stable CNFs-based transparent films for industrial application in the fields of photonics and electronics	1052:1169	This work provided a practical and effective way for preparation of photothermal-stable CNFs-based transparent films for industrial application in the fields of photonics and electronics.
33858576	3	42	from	investigation	424:436	arg1	stability					558:566	the thermal stability	546:566	the thermal stability of CNFs/ZnAl-LDHs composited films	546:601	This work mainly focused on investigation of the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films.
33858576	4	43	theme	photothermal	686:697	arg1	stability					699:707	the photothermal stability	682:707	the photothermal stability of CNFs-based films	682:727	The results showed that the morphology of LDHs was positively correlated with the photothermal stability of CNFs-based films.
33858576	3	44	theme	influence	445:453	arg1	investigation					424:436	investigation	424:436	investigation of the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films	424:601	This work mainly focused on investigation of the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films.
33858576	3	45	theme	thermal	550:556	arg1	stability					558:566	the thermal stability	546:566	the thermal stability of CNFs/ZnAl-LDHs composited films	546:601	This work mainly focused on investigation of the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films.
33858576	2	46	theme	hydroxides	321:330	arg1	nanopowders					339:349	layered double hydroxides (LDHs) nanopowders	306:349	layered double hydroxides (LDHs) nanopowders	306:349	This problem could be solved by doping layered double hydroxides (LDHs) nanopowders with opposite charge on the surface of CNFs.
33858576	5	47	with	ZnAl-LDHs	745:753	arg1	morphology					776:785	uniform crystal morphology	760:785	uniform crystal morphology	760:785	Specially, the ZnAl-LDHs with uniform crystal morphology could be prepared by controlling the molar ratio of Zn/Al at 3:1 and thus enhance the photothermal stability of CNFs-based films without any serious light transmittance deteriorating after doping.
33858576	3	48	theme	films	597:601	arg1	stability					558:566	the thermal stability	546:566	the thermal stability of CNFs/ZnAl-LDHs composited films	546:601	This work mainly focused on investigation of the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films.
33858576	2	49	theme	double	314:319	arg1	nanopowders					339:349	layered double hydroxides (LDHs) nanopowders	306:349	layered double hydroxides (LDHs) nanopowders	306:349	This problem could be solved by doping layered double hydroxides (LDHs) nanopowders with opposite charge on the surface of CNFs.
33858576	6	50	theme	electronics	1159:1169	arg1	fields					1135:1140	the fields	1131:1140	the fields of photonics and electronics	1131:1169	This work provided a practical and effective way for preparation of photothermal-stable CNFs-based transparent films for industrial application in the fields of photonics and electronics.
33858576	6	51	theme	effective	1019:1027	arg1	way					1029:1031	a practical and effective way	1003:1031	a practical and effective way for preparation of photothermal-stable CNFs-based transparent films for industrial application in the fields of photonics and electronics	1003:1169	This work provided a practical and effective way for preparation of photothermal-stable CNFs-based transparent films for industrial application in the fields of photonics and electronics.
33858576	5	52	from	3:1	848:850	arg1	ratio					830:834	the molar ratio	820:834	the molar ratio of Zn/Al at 3:1	820:850	Specially, the ZnAl-LDHs with uniform crystal morphology could be prepared by controlling the molar ratio of Zn/Al at 3:1 and thus enhance the photothermal stability of CNFs-based films without any serious light transmittance deteriorating after doping.
33858576	1	53	theme	hydroxyl	130:137	arg1	groups					152:157	hydroxyl and carboxyl groups	130:157	groups	152:157	The existence of hydroxyl and carboxyl groups makes the photothermal stability of cellulose nanofibers (CNFs) poor and thus limits its scale application.
33858576	1	54	theme	poor	223:226	arg1	stability					182:190	the photothermal stability	165:190	the photothermal stability of cellulose nanofibers (CNFs) poor	165:226	The existence of hydroxyl and carboxyl groups makes the photothermal stability of cellulose nanofibers (CNFs) poor and thus limits its scale application.
33858576	3	55	theme	CNFs/ZnAl-LDHs	571:584	arg1	films					597:601	CNFs/ZnAl-LDHs composited films	571:601	CNFs/ZnAl-LDHs composited films	571:601	This work mainly focused on investigation of the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films.
33858576	6	56	theme	transparent	1083:1093	arg1	films					1095:1099	photothermal-stable CNFs-based transparent films	1052:1099	photothermal-stable CNFs-based transparent films for industrial application in the fields of photonics and electronics	1052:1169	This work provided a practical and effective way for preparation of photothermal-stable CNFs-based transparent films for industrial application in the fields of photonics and electronics.
33858576	6	57	theme	practical	1005:1013	arg1	way					1029:1031	a practical and effective way	1003:1031	a practical and effective way for preparation of photothermal-stable CNFs-based transparent films for industrial application in the fields of photonics and electronics	1003:1169	This work provided a practical and effective way for preparation of photothermal-stable CNFs-based transparent films for industrial application in the fields of photonics and electronics.
33858576	3	58	theme	composited	586:595	arg1	films					597:601	CNFs/ZnAl-LDHs composited films	571:601	CNFs/ZnAl-LDHs composited films	571:601	This work mainly focused on investigation of the influence of the crystal morphology of the inorganic ultraviolet shielding agent (i.e. ZnAl-LDHs) on the thermal stability of CNFs/ZnAl-LDHs composited films.
33858576	1	59	theme	carboxyl	143:150	arg1	groups					152:157	hydroxyl and carboxyl groups	130:157	groups	152:157	The existence of hydroxyl and carboxyl groups makes the photothermal stability of cellulose nanofibers (CNFs) poor and thus limits its scale application.
32745551	0	0	theme	composite	101:109	arg1	films					111:115	nanocellulose/CMC and nanochitosan/CMC composite films	62:115	nanocellulose/CMC and nanochitosan/CMC composite films	62:115	Comparing mechanical, barrier and antimicrobial properties of nanocellulose/CMC and nanochitosan/CMC composite films.
32745551	5	1	theme	polymer	638:644	arg1	permeability					622:633	Water vapor permeability	610:633	Water vapor permeability of polymer and nanofiller	610:659	Water vapor permeability of polymer and nanofiller decreased when nanocomposite concentration increased.
32745551	9	2	theme	nanofiller	1054:1063	arg1	aggregation					1039:1049	aggregation	1039:1049	aggregation of nanofiller in CMC film	1039:1075	However, in high content (1%) cause to create aggregation of nanofiller in CMC film.
32745551	0	3	theme	nanochitosan/CMC	84:99	arg1	films					111:115	nanocellulose/CMC and nanochitosan/CMC composite films	62:115	nanocellulose/CMC and nanochitosan/CMC composite films	62:115	Comparing mechanical, barrier and antimicrobial properties of nanocellulose/CMC and nanochitosan/CMC composite films.
32745551	6	4	from	break	750:754	arg1	Elongation					736:745	Elongation	736:745	Elongation	736:745	Tensile strength and Elongation at break improved in nanocomposite film by increasing concentration.
32745551	6	4	from	break	750:754	arg1	strength					723:730	Tensile strength	715:730	Tensile strength	715:730	Tensile strength and Elongation at break improved in nanocomposite film by increasing concentration.
32745551	10	5	theme	inhibitory	1219:1228	arg1	effect					1230:1235	no inhibitory effect	1216:1235	no inhibitory effect	1216:1235	Finally antibacterial activity against five pathogens was studied and good effective inhibition on CMC/NCH was observed while CMC/NCL had no inhibitory effect.
32745551	9	6	theme	high	1005:1008	arg1	content					1010:1016	high content	1005:1016	high content (1%)	1005:1021	However, in high content (1%) cause to create aggregation of nanofiller in CMC film.
32745551	9	6	theme	high	1005:1008	arg1	%					1020:1020	1%	1019:1020	1%	1019:1020	However, in high content (1%) cause to create aggregation of nanofiller in CMC film.
32745551	5	7	theme	nanofiller	650:659	arg1	permeability					622:633	Water vapor permeability	610:633	Water vapor permeability of polymer and nanofiller	610:659	Water vapor permeability of polymer and nanofiller decreased when nanocomposite concentration increased.
32745551	0	8	theme	films	111:115	arg1	properties					48:57	mechanical, barrier and antimicrobial properties	10:57	properties	48:57	Comparing mechanical, barrier and antimicrobial properties of nanocellulose/CMC and nanochitosan/CMC composite films.
32745551	5	9	theme	Water	610:614	arg1	permeability					622:633	Water vapor permeability	610:633	Water vapor permeability of polymer and nanofiller	610:659	Water vapor permeability of polymer and nanofiller decreased when nanocomposite concentration increased.
32745551	11	10	theme	CMC/NCL	1326:1332	arg1	biopolymer					1334:1343	CMC/NCL biopolymer	1326:1343	CMC/NCL biopolymer	1326:1343	These results show that use of CMC/NCH as a biocompatible film has more advantages than CMC/NCL biopolymer.
32745551	1	11	theme	cellulose	164:172	arg1	nano-cellulose					139:152	nano-cellulose	139:152	nano-cellulose (NCL)	139:158	Nano-chitosan (NCH), nano-cellulose (NCL) and cellulose derivative are biodegradable biopolymer.
32745551	1	11	theme	cellulose	164:172	arg1	Nano-chitosan					118:130	Nano-chitosan	118:130	Nano-chitosan (NCH)	118:136	Nano-chitosan (NCH), nano-cellulose (NCL) and cellulose derivative are biodegradable biopolymer.
32745551	1	11	theme	cellulose	164:172	arg1	derivative					174:183	cellulose derivative	164:183	cellulose derivative	164:183	Nano-chitosan (NCH), nano-cellulose (NCL) and cellulose derivative are biodegradable biopolymer.
32745551	1	11	theme	cellulose	164:172	arg1	biopolymer					203:212	biodegradable biopolymer	189:212	biodegradable biopolymer	189:212	Nano-chitosan (NCH), nano-cellulose (NCL) and cellulose derivative are biodegradable biopolymer.
32745551	3	12	theme	physical	407:414	arg1	solubility					434:443	water solubility	428:443	water solubility	428:443	Both CMC/NCH and NCL decreased physical properties (water solubility, moisture content and moisture absorption) especially in concentration of 1%.
32745551	3	12	theme	physical	407:414	arg1	content					455:461	moisture content	446:461	moisture content	446:461	Both CMC/NCH and NCL decreased physical properties (water solubility, moisture content and moisture absorption) especially in concentration of 1%.
32745551	3	12	theme	physical	407:414	arg1	absorption					476:485	moisture absorption	467:485	moisture absorption	467:485	Both CMC/NCH and NCL decreased physical properties (water solubility, moisture content and moisture absorption) especially in concentration of 1%.
32745551	3	12	theme	physical	407:414	arg1	properties					416:425	physical properties	407:425	physical properties (water solubility, moisture content and moisture absorption)	407:486	Both CMC/NCH and NCL decreased physical properties (water solubility, moisture content and moisture absorption) especially in concentration of 1%.
32745551	10	13	contain	had	1212:1214	arg2	effect					1230:1235	no inhibitory effect	1216:1235	no inhibitory effect	1216:1235	Finally antibacterial activity against five pathogens was studied and good effective inhibition on CMC/NCH was observed while CMC/NCL had no inhibitory effect.
32745551	10	13	contain	had	1212:1214	arg1	CMC/NCL					1204:1210	CMC/NCL	1204:1210	CMC/NCL	1204:1210	Finally antibacterial activity against five pathogens was studied and good effective inhibition on CMC/NCH was observed while CMC/NCL had no inhibitory effect.
32745551	2	14	from	concentrations	260:273	arg1	nano-cellulose					232:245	nano-cellulose	232:245	nano-cellulose	232:245	Nano-chitosan or nano-cellulose at different concentrations (0.1, 0.5 and 1%) incorporated in carboxymethyl cellulose (CMC) film solution using casting methods.
32745551	2	14	from	concentrations	260:273	arg1	Nano-chitosan					215:227	Nano-chitosan	215:227	Nano-chitosan	215:227	Nano-chitosan or nano-cellulose at different concentrations (0.1, 0.5 and 1%) incorporated in carboxymethyl cellulose (CMC) film solution using casting methods.
32745551	0	15	theme	mechanical	10:19	arg1	properties					48:57	mechanical, barrier and antimicrobial properties	10:57	properties	48:57	Comparing mechanical, barrier and antimicrobial properties of nanocellulose/CMC and nanochitosan/CMC composite films.
32745551	8	16	theme	peaks	920:924	arg1	Emersion					896:903	Emersion	896:903	Emersion of crystalline peaks in X-ray analyses	896:942	Emersion of crystalline peaks in X-ray analyses certified the presence of nanofiller in polymer.
32745551	10	17	theme	good	1148:1151	arg1	inhibition					1163:1172	good effective inhibition	1148:1172	good effective inhibition on CMC/NCH	1148:1183	Finally antibacterial activity against five pathogens was studied and good effective inhibition on CMC/NCH was observed while CMC/NCL had no inhibitory effect.
32745551	2	18	theme	cellulose	323:331	arg1	solution					344:351	carboxymethyl cellulose (CMC) film solution	309:351	carboxymethyl cellulose (CMC) film solution using casting methods	309:373	Nano-chitosan or nano-cellulose at different concentrations (0.1, 0.5 and 1%) incorporated in carboxymethyl cellulose (CMC) film solution using casting methods.
32745551	8	19	theme	crystalline	908:918	arg1	peaks					920:924	crystalline peaks	908:924	crystalline peaks	908:924	Emersion of crystalline peaks in X-ray analyses certified the presence of nanofiller in polymer.
32745551	8	20	from	presence	958:965	arg1	polymer					984:990	polymer	984:990	polymer	984:990	Emersion of crystalline peaks in X-ray analyses certified the presence of nanofiller in polymer.
32745551	7	21	theme	CMC/NCH	838:844	arg1	properties					824:833	Thermal properties	816:833	Thermal properties of CMC/NCH	816:844	Thermal properties of CMC/NCH were significantly (p < 0.05) lower than CMC/NCL.
32745551	3	22	dep	properties	416:425	arg1	solubility					434:443	water solubility	428:443	water solubility	428:443	Both CMC/NCH and NCL decreased physical properties (water solubility, moisture content and moisture absorption) especially in concentration of 1%.
32745551	3	22	dep	properties	416:425	arg1	content					455:461	moisture content	446:461	moisture content	446:461	Both CMC/NCH and NCL decreased physical properties (water solubility, moisture content and moisture absorption) especially in concentration of 1%.
32745551	3	22	dep	properties	416:425	arg1	absorption					476:485	moisture absorption	467:485	moisture absorption	467:485	Both CMC/NCH and NCL decreased physical properties (water solubility, moisture content and moisture absorption) especially in concentration of 1%.
32745551	3	22	dep	properties	416:425	arg1	properties					416:425	physical properties	407:425	physical properties (water solubility, moisture content and moisture absorption)	407:486	Both CMC/NCH and NCL decreased physical properties (water solubility, moisture content and moisture absorption) especially in concentration of 1%.
32745551	2	23	theme	carboxymethyl	309:321	arg1	solution					344:351	carboxymethyl cellulose (CMC) film solution	309:351	carboxymethyl cellulose (CMC) film solution using casting methods	309:373	Nano-chitosan or nano-cellulose at different concentrations (0.1, 0.5 and 1%) incorporated in carboxymethyl cellulose (CMC) film solution using casting methods.
32745551	8	24	theme	X-ray	929:933	arg1	analyses					935:942	X-ray analyses	929:942	X-ray analyses	929:942	Emersion of crystalline peaks in X-ray analyses certified the presence of nanofiller in polymer.
32745551	3	25	theme	water	428:432	arg1	solubility					434:443	water solubility	428:443	water solubility	428:443	Both CMC/NCH and NCL decreased physical properties (water solubility, moisture content and moisture absorption) especially in concentration of 1%.
32745551	3	25	theme	water	428:432	arg1	properties					416:425	physical properties	407:425	physical properties (water solubility, moisture content and moisture absorption)	407:486	Both CMC/NCH and NCL decreased physical properties (water solubility, moisture content and moisture absorption) especially in concentration of 1%.
32745551	0	26	theme	barrier	22:28	arg1	properties					48:57	mechanical, barrier and antimicrobial properties	10:57	properties	48:57	Comparing mechanical, barrier and antimicrobial properties of nanocellulose/CMC and nanochitosan/CMC composite films.
32745551	8	27	attach	presence	958:965	arg1	polymer					984:990	polymer	984:990	polymer	984:990	Emersion of crystalline peaks in X-ray analyses certified the presence of nanofiller in polymer.
32745551	8	27	attach	presence	958:965	arg2	nanofiller					970:979	nanofiller	970:979	nanofiller	970:979	Emersion of crystalline peaks in X-ray analyses certified the presence of nanofiller in polymer.
32745551	8	28	from	Emersion	896:903	arg1	analyses					935:942	X-ray analyses	929:942	X-ray analyses	929:942	Emersion of crystalline peaks in X-ray analyses certified the presence of nanofiller in polymer.
32745551	4	29	from	properties	538:547	arg1	CMC/NCH					552:558	CMC/NCH	552:558	CMC/NCH	552:558	However, these properties in CMC/NCH were significantly (p < 0.05) lower than CMC/NCL.
32745551	8	30	theme	nanofiller	970:979	arg1	presence					958:965	the presence	954:965	the presence of nanofiller in polymer	954:990	Emersion of crystalline peaks in X-ray analyses certified the presence of nanofiller in polymer.
32745551	10	31	from	inhibition	1163:1172	arg1	CMC/NCH					1177:1183	CMC/NCH	1177:1183	CMC/NCH	1177:1183	Finally antibacterial activity against five pathogens was studied and good effective inhibition on CMC/NCH was observed while CMC/NCL had no inhibitory effect.
32745551	3	32	theme	moisture	467:474	arg1	absorption					476:485	moisture absorption	467:485	moisture absorption	467:485	Both CMC/NCH and NCL decreased physical properties (water solubility, moisture content and moisture absorption) especially in concentration of 1%.
32745551	3	32	theme	moisture	467:474	arg1	properties					416:425	physical properties	407:425	physical properties (water solubility, moisture content and moisture absorption)	407:486	Both CMC/NCH and NCL decreased physical properties (water solubility, moisture content and moisture absorption) especially in concentration of 1%.
32745551	0	33	theme	antimicrobial	34:46	arg1	properties					48:57	mechanical, barrier and antimicrobial properties	10:57	properties	48:57	Comparing mechanical, barrier and antimicrobial properties of nanocellulose/CMC and nanochitosan/CMC composite films.
32745551	9	34	from	aggregation	1039:1049	arg1	CMC					1068:1070	CMC film	1068:1075	CMC film	1068:1075	However, in high content (1%) cause to create aggregation of nanofiller in CMC film.
32745551	11	35	theme	CMC/NCH	1269:1275	arg1	use					1262:1264	use	1262:1264	use of CMC/NCH as a biocompatible film	1262:1299	These results show that use of CMC/NCH as a biocompatible film has more advantages than CMC/NCL biopolymer.
32745551	1	36	theme	biodegradable	189:201	arg1	nano-cellulose					139:152	nano-cellulose	139:152	nano-cellulose (NCL)	139:158	Nano-chitosan (NCH), nano-cellulose (NCL) and cellulose derivative are biodegradable biopolymer.
32745551	1	36	theme	biodegradable	189:201	arg1	Nano-chitosan					118:130	Nano-chitosan	118:130	Nano-chitosan (NCH)	118:136	Nano-chitosan (NCH), nano-cellulose (NCL) and cellulose derivative are biodegradable biopolymer.
32745551	1	36	theme	biodegradable	189:201	arg1	derivative					174:183	cellulose derivative	164:183	cellulose derivative	164:183	Nano-chitosan (NCH), nano-cellulose (NCL) and cellulose derivative are biodegradable biopolymer.
32745551	1	36	theme	biodegradable	189:201	arg1	biopolymer					203:212	biodegradable biopolymer	189:212	biodegradable biopolymer	189:212	Nano-chitosan (NCH), nano-cellulose (NCL) and cellulose derivative are biodegradable biopolymer.
32745551	3	37	theme	moisture	446:453	arg1	content					455:461	moisture content	446:461	moisture content	446:461	Both CMC/NCH and NCL decreased physical properties (water solubility, moisture content and moisture absorption) especially in concentration of 1%.
32745551	3	37	theme	moisture	446:453	arg1	properties					416:425	physical properties	407:425	physical properties (water solubility, moisture content and moisture absorption)	407:486	Both CMC/NCH and NCL decreased physical properties (water solubility, moisture content and moisture absorption) especially in concentration of 1%.
32745551	2	38	theme	different	250:258	arg1	%					290:290	0.1, 0.5 and 1%	276:290	0.1, 0.5 and 1%	276:290	Nano-chitosan or nano-cellulose at different concentrations (0.1, 0.5 and 1%) incorporated in carboxymethyl cellulose (CMC) film solution using casting methods.
32745551	2	38	theme	different	250:258	arg1	concentrations					260:273	different concentrations	250:273	different concentrations (0.1, 0.5 and 1%)	250:291	Nano-chitosan or nano-cellulose at different concentrations (0.1, 0.5 and 1%) incorporated in carboxymethyl cellulose (CMC) film solution using casting methods.
32745551	7	39	dep	lower	876:880	arg1	p < 0.05					866:873	p < 0.05	866:873	p < 0.05	866:873	Thermal properties of CMC/NCH were significantly (p < 0.05) lower than CMC/NCL.
32745551	7	40	theme	Thermal	816:822	arg1	properties					824:833	Thermal properties	816:833	Thermal properties of CMC/NCH	816:844	Thermal properties of CMC/NCH were significantly (p < 0.05) lower than CMC/NCL.
32745551	2	41	theme	film	339:342	arg1	solution					344:351	carboxymethyl cellulose (CMC) film solution	309:351	carboxymethyl cellulose (CMC) film solution using casting methods	309:373	Nano-chitosan or nano-cellulose at different concentrations (0.1, 0.5 and 1%) incorporated in carboxymethyl cellulose (CMC) film solution using casting methods.
32745551	5	42	theme	nanocomposite	676:688	arg1	concentration					690:702	nanocomposite concentration	676:702	nanocomposite concentration	676:702	Water vapor permeability of polymer and nanofiller decreased when nanocomposite concentration increased.
32745551	5	43	theme	vapor	616:620	arg1	permeability					622:633	Water vapor permeability	610:633	Water vapor permeability of polymer and nanofiller	610:659	Water vapor permeability of polymer and nanofiller decreased when nanocomposite concentration increased.
32745551	6	44	theme	nanocomposite	768:780	arg1	film					782:785	nanocomposite film	768:785	nanocomposite film	768:785	Tensile strength and Elongation at break improved in nanocomposite film by increasing concentration.
32745551	10	45	theme	effective	1153:1161	arg1	inhibition					1163:1172	good effective inhibition	1148:1172	good effective inhibition on CMC/NCH	1148:1183	Finally antibacterial activity against five pathogens was studied and good effective inhibition on CMC/NCH was observed while CMC/NCL had no inhibitory effect.
32745551	2	46	theme	CMC	334:336	arg1	solution					344:351	carboxymethyl cellulose (CMC) film solution	309:351	carboxymethyl cellulose (CMC) film solution using casting methods	309:373	Nano-chitosan or nano-cellulose at different concentrations (0.1, 0.5 and 1%) incorporated in carboxymethyl cellulose (CMC) film solution using casting methods.
32745551	2	47	theme	casting	359:365	arg1	methods					367:373	casting methods	359:373	casting methods	359:373	Nano-chitosan or nano-cellulose at different concentrations (0.1, 0.5 and 1%) incorporated in carboxymethyl cellulose (CMC) film solution using casting methods.
32745551	6	48	theme	Tensile	715:721	arg1	strength					723:730	Tensile strength	715:730	Tensile strength	715:730	Tensile strength and Elongation at break improved in nanocomposite film by increasing concentration.
32745551	4	49	dep	lower	590:594	arg1	p < 0.05					580:587	p < 0.05	580:587	p < 0.05	580:587	However, these properties in CMC/NCH were significantly (p < 0.05) lower than CMC/NCL.
32745551	11	50	theme	biocompatible	1282:1294	arg1	film					1296:1299	a biocompatible film	1280:1299	a biocompatible film	1280:1299	These results show that use of CMC/NCH as a biocompatible film has more advantages than CMC/NCL biopolymer.
32745551	0	51	theme	nanocellulose/CMC	62:78	arg1	films					111:115	nanocellulose/CMC and nanochitosan/CMC composite films	62:115	nanocellulose/CMC and nanochitosan/CMC composite films	62:115	Comparing mechanical, barrier and antimicrobial properties of nanocellulose/CMC and nanochitosan/CMC composite films.
32745551	10	52	theme	antibacterial	1086:1098	arg1	activity					1100:1107	antibacterial activity	1086:1107	antibacterial activity against five pathogens	1086:1130	Finally antibacterial activity against five pathogens was studied and good effective inhibition on CMC/NCH was observed while CMC/NCL had no inhibitory effect.
32745551	3	53	theme	%	520:520	arg1	concentration					502:514	concentration	502:514	concentration of 1%	502:520	Both CMC/NCH and NCL decreased physical properties (water solubility, moisture content and moisture absorption) especially in concentration of 1%.
33775355	5	0	theme	grain	730:734	arg1	development					736:746	the wheat grain development	720:746	the wheat grain development	720:746	While studying the wheat grain development, the cavity content was found to autofluoresce under UV light excitation.
33775355	1	1	theme	calories	133:140	arg1	part					121:124	a substantial part	107:124	a substantial part of the calories for humans and animals	107:163	Cereal grains provide a substantial part of the calories for humans and animals.
33775355	7	2	theme	dry	1106:1108	arg1	grain					1117:1121	dry mature grain	1106:1121	dry mature grain	1106:1121	The cavity content forms a "gel" in the developing grain, which persists in dry mature grain and during subsequent imbibition.
33775355	0	3	contain	contains	37:44	arg1	cavity					14:19	The endosperm cavity	0:19	The endosperm cavity of wheat grains	0:35	The endosperm cavity of wheat grains contains a highly hydrated gel of arabinoxylan.
33775355	0	3	contain	contains	37:44	arg2	gel					64:66	a highly hydrated gel	46:66	a highly hydrated gel of arabinoxylan	46:82	The endosperm cavity of wheat grains contains a highly hydrated gel of arabinoxylan.
33775355	7	4	from	"	1061:1061	arg1	grain					1081:1085	the developing grain	1066:1085	the developing grain	1066:1085	The cavity content forms a "gel" in the developing grain, which persists in dry mature grain and during subsequent imbibition.
33775355	6	5	theme	multispectral	832:844	arg1	immunolabeling					897:910	immunolabeling	897:910	immunolabeling	897:910	Combining multispectral analysis, Fourier-Transform infrared spectroscopy, immunolabeling and laser-dissection coupled with wet chemistry, we identified in the cavity arabinoxylans and hydroxycinnamic acids.
33775355	6	5	theme	multispectral	832:844	arg1	spectroscopy					883:894	Fourier-Transform infrared spectroscopy	856:894	Fourier-Transform infrared spectroscopy	856:894	Combining multispectral analysis, Fourier-Transform infrared spectroscopy, immunolabeling and laser-dissection coupled with wet chemistry, we identified in the cavity arabinoxylans and hydroxycinnamic acids.
33775355	6	5	theme	multispectral	832:844	arg1	laser-dissection					916:931	laser-dissection	916:931	laser-dissection	916:931	Combining multispectral analysis, Fourier-Transform infrared spectroscopy, immunolabeling and laser-dissection coupled with wet chemistry, we identified in the cavity arabinoxylans and hydroxycinnamic acids.
33775355	6	5	theme	multispectral	832:844	arg1	analysis					846:853	multispectral analysis	832:853	multispectral analysis	832:853	Combining multispectral analysis, Fourier-Transform infrared spectroscopy, immunolabeling and laser-dissection coupled with wet chemistry, we identified in the cavity arabinoxylans and hydroxycinnamic acids.
33775355	3	6	theme	vascular	505:512	arg1	tissues					514:520	the vascular tissues	501:520	the vascular tissues of the maternal plant	501:542	In this study, the composition of a structure localized at the interface of the vascular tissues of the maternal plant and the seed endosperm was investigated.
33775355	6	7	theme	cavity	982:987	arg1	arabinoxylans					989:1001	the cavity arabinoxylans	978:1001	the cavity arabinoxylans	978:1001	Combining multispectral analysis, Fourier-Transform infrared spectroscopy, immunolabeling and laser-dissection coupled with wet chemistry, we identified in the cavity arabinoxylans and hydroxycinnamic acids.
33775355	0	8	theme	arabinoxylan	71:82	arg1	gel					64:66	a highly hydrated gel	46:66	a highly hydrated gel of arabinoxylan	46:82	The endosperm cavity of wheat grains contains a highly hydrated gel of arabinoxylan.
33775355	9	9	theme	nucellar	1300:1307	arg1	epidermis					1309:1317	the nucellar epidermis	1296:1317	the nucellar epidermis	1296:1317	Our results suggest that arabinoxylans are synthesized by the nucellar epidermis, released in the cavity where they form a highly hydrated gel which might contribute to regulate grain hydration.
33775355	3	10	theme	tissues	514:520	arg1	interface					488:496	the interface	484:496	the interface of the vascular tissues of the maternal plant	484:542	In this study, the composition of a structure localized at the interface of the vascular tissues of the maternal plant and the seed endosperm was investigated.
33775355	9	11	theme	grain	1416:1420	arg1	hydration					1422:1430	grain hydration	1416:1430	grain hydration	1416:1430	Our results suggest that arabinoxylans are synthesized by the nucellar epidermis, released in the cavity where they form a highly hydrated gel which might contribute to regulate grain hydration.
33775355	8	12	theme	Microscopic	1157:1167	arg1	imaging					1188:1194	Microscopic magnetic resonance imaging	1157:1194	Microscopic magnetic resonance imaging	1157:1194	Microscopic magnetic resonance imaging revealed that the gel is highly hydrated.
33775355	6	13	theme	infrared	874:881	arg1	spectroscopy					883:894	Fourier-Transform infrared spectroscopy	856:894	Fourier-Transform infrared spectroscopy	856:894	Combining multispectral analysis, Fourier-Transform infrared spectroscopy, immunolabeling and laser-dissection coupled with wet chemistry, we identified in the cavity arabinoxylans and hydroxycinnamic acids.
33775355	6	13	theme	infrared	874:881	arg1	analysis					846:853	multispectral analysis	832:853	multispectral analysis	832:853	Combining multispectral analysis, Fourier-Transform infrared spectroscopy, immunolabeling and laser-dissection coupled with wet chemistry, we identified in the cavity arabinoxylans and hydroxycinnamic acids.
33775355	2	14	theme	dietary	250:256	arg1	fibers					258:263	starch but also dietary fibers	234:263	mainly starch but also dietary fibers such as arabinoxylans	227:285	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	2	14	theme	dietary	250:256	arg1	arabinoxylans					273:285	arabinoxylans	273:285	arabinoxylans	273:285	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	2	14	theme	dietary	250:256	arg1	glucans					302:308	mixed-linkage glucans	288:308	mixed-linkage glucans	288:308	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	4	15	theme	endosperm	620:628	arg1	cavity					630:635	the endosperm cavity	616:635	the endosperm cavity where water and nutrients are transferred to support grain filling	616:702	This structure is contained in the endosperm cavity where water and nutrients are transferred to support grain filling.
33775355	7	16	theme	mature	1110:1115	arg1	grain					1117:1121	dry mature grain	1106:1121	dry mature grain	1106:1121	The cavity content forms a "gel" in the developing grain, which persists in dry mature grain and during subsequent imbibition.
33775355	6	17	theme	Fourier-Transform	856:872	arg1	spectroscopy					883:894	Fourier-Transform infrared spectroscopy	856:894	Fourier-Transform infrared spectroscopy	856:894	Combining multispectral analysis, Fourier-Transform infrared spectroscopy, immunolabeling and laser-dissection coupled with wet chemistry, we identified in the cavity arabinoxylans and hydroxycinnamic acids.
33775355	6	17	theme	Fourier-Transform	856:872	arg1	analysis					846:853	multispectral analysis	832:853	multispectral analysis	832:853	Combining multispectral analysis, Fourier-Transform infrared spectroscopy, immunolabeling and laser-dissection coupled with wet chemistry, we identified in the cavity arabinoxylans and hydroxycinnamic acids.
33775355	0	18	theme	endosperm	4:12	arg1	cavity					14:19	The endosperm cavity	0:19	The endosperm cavity of wheat grains	0:35	The endosperm cavity of wheat grains contains a highly hydrated gel of arabinoxylan.
33775355	2	19	theme	mixed-linkage	288:300	arg1	fibers					258:263	starch but also dietary fibers	234:263	mainly starch but also dietary fibers such as arabinoxylans	227:285	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	2	19	theme	mixed-linkage	288:300	arg1	arabinoxylans					273:285	arabinoxylans	273:285	arabinoxylans	273:285	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	2	19	theme	mixed-linkage	288:300	arg1	glucans					302:308	mixed-linkage glucans	288:308	mixed-linkage glucans	288:308	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	8	20	theme	magnetic	1169:1176	arg1	imaging					1188:1194	Microscopic magnetic resonance imaging	1157:1194	Microscopic magnetic resonance imaging	1157:1194	Microscopic magnetic resonance imaging revealed that the gel is highly hydrated.
33775355	7	21	theme	developing	1070:1079	arg1	grain					1081:1085	the developing grain	1066:1085	the developing grain	1066:1085	The cavity content forms a "gel" in the developing grain, which persists in dry mature grain and during subsequent imbibition.
33775355	3	22	theme	plant	538:542	arg1	tissues					514:520	the vascular tissues	501:520	the vascular tissues of the maternal plant	501:542	In this study, the composition of a structure localized at the interface of the vascular tissues of the maternal plant and the seed endosperm was investigated.
33775355	2	23	theme	grain	359:363	arg1	development					365:375	grain development	359:375	grain development	359:375	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	5	24	theme	light	804:808	arg1	excitation					810:819	UV light excitation	801:819	UV light excitation	801:819	While studying the wheat grain development, the cavity content was found to autofluoresce under UV light excitation.
33775355	2	25	dep	proteins	315:322	arg1	accumulated					340:350	accumulated	340:350	accumulated during grain development in a specialized storage tissue	340:407	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	2	25	dep	proteins	315:322	arg1	synthesized					324:334	synthesized	324:334	synthesized	324:334	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	5	26	theme	UV	801:802	arg1	excitation					810:819	UV light excitation	801:819	UV light excitation	801:819	While studying the wheat grain development, the cavity content was found to autofluoresce under UV light excitation.
33775355	0	27	theme	grains	30:35	arg1	cavity					14:19	The endosperm cavity	0:19	The endosperm cavity of wheat grains	0:35	The endosperm cavity of wheat grains contains a highly hydrated gel of arabinoxylan.
33775355	2	28	theme	storage	394:400	arg1	tissue					402:407	a specialized storage tissue	380:407	a specialized storage tissue	380:407	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	3	29	theme	seed	552:555	arg1	endosperm					557:565	the seed endosperm	548:565	the seed endosperm	548:565	In this study, the composition of a structure localized at the interface of the vascular tissues of the maternal plant and the seed endosperm was investigated.
33775355	2	30	dep	polysaccharides	210:224	arg1	fibers					258:263	starch but also dietary fibers	234:263	mainly starch but also dietary fibers such as arabinoxylans	227:285	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	2	30	dep	polysaccharides	210:224	arg1	arabinoxylans					273:285	arabinoxylans	273:285	arabinoxylans	273:285	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	2	30	dep	polysaccharides	210:224	arg1	glucans					302:308	mixed-linkage glucans	288:308	mixed-linkage glucans	288:308	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	2	30	dep	polysaccharides	210:224	arg1	endosperm					414:422	the endosperm	410:422	the endosperm	410:422	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	0	31	theme	wheat	24:28	arg1	grains					30:35	wheat grains	24:35	wheat grains	24:35	The endosperm cavity of wheat grains contains a highly hydrated gel of arabinoxylan.
33775355	7	32	theme	gel	1058:1060	arg1	"					1061:1061	a "gel"	1055:1061	a "gel"	1055:1061	The cavity content forms a "gel" in the developing grain, which persists in dry mature grain and during subsequent imbibition.
33775355	7	33	theme	subsequent	1134:1143	arg1	imbibition					1145:1154	subsequent imbibition	1134:1154	subsequent imbibition	1134:1154	The cavity content forms a "gel" in the developing grain, which persists in dry mature grain and during subsequent imbibition.
33775355	2	34	theme	specialized	382:392	arg1	tissue					402:407	a specialized storage tissue	380:407	a specialized storage tissue	380:407	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	5	35	theme	cavity	753:758	arg1	content					760:766	the cavity content	749:766	the cavity content	749:766	While studying the wheat grain development, the cavity content was found to autofluoresce under UV light excitation.
33775355	6	36	theme	wet	946:948	arg1	chemistry					950:958	wet chemistry	946:958	wet chemistry	946:958	Combining multispectral analysis, Fourier-Transform infrared spectroscopy, immunolabeling and laser-dissection coupled with wet chemistry, we identified in the cavity arabinoxylans and hydroxycinnamic acids.
33775355	2	37	theme	grains	199:204	arg1	polysaccharides					210:224	polysaccharides	210:224	polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans)	210:309	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	2	37	theme	grains	199:204	arg1	determinants					183:194	The main quality determinants	166:194	The main quality determinants of grains	166:204	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	3	38	theme	structure	461:469	arg1	composition					444:454	the composition	440:454	the composition of a structure localized at the interface of the vascular tissues of the maternal plant	440:542	In this study, the composition of a structure localized at the interface of the vascular tissues of the maternal plant and the seed endosperm was investigated.
33775355	3	38	theme	structure	461:469	arg1	endosperm					557:565	the seed endosperm	548:565	the seed endosperm	548:565	In this study, the composition of a structure localized at the interface of the vascular tissues of the maternal plant and the seed endosperm was investigated.
33775355	3	39	theme	maternal	529:536	arg1	plant					538:542	the maternal plant	525:542	the maternal plant	525:542	In this study, the composition of a structure localized at the interface of the vascular tissues of the maternal plant and the seed endosperm was investigated.
33775355	7	40	theme	cavity	1034:1039	arg1	content					1041:1047	The cavity content	1030:1047	The cavity content	1030:1047	The cavity content forms a "gel" in the developing grain, which persists in dry mature grain and during subsequent imbibition.
33775355	2	41	theme	quality	175:181	arg1	polysaccharides					210:224	polysaccharides	210:224	polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans)	210:309	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	2	41	theme	quality	175:181	arg1	determinants					183:194	The main quality determinants	166:194	The main quality determinants of grains	166:204	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	3	42	located	localized	471:479	arg2	structure					461:469	a structure	459:469	a structure localized at the interface of the vascular tissues of the maternal plant	459:542	In this study, the composition of a structure localized at the interface of the vascular tissues of the maternal plant and the seed endosperm was investigated.
33775355	3	42	located	localized	471:479	arg1	interface					488:496	the interface	484:496	the interface of the vascular tissues of the maternal plant	484:542	In this study, the composition of a structure localized at the interface of the vascular tissues of the maternal plant and the seed endosperm was investigated.
33775355	1	43	theme	substantial	109:119	arg1	part					121:124	a substantial part	107:124	a substantial part of the calories for humans and animals	107:163	Cereal grains provide a substantial part of the calories for humans and animals.
33775355	1	44	theme	Cereal	85:90	arg1	grains					92:97	Cereal grains	85:97	Cereal grains	85:97	Cereal grains provide a substantial part of the calories for humans and animals.
33775355	9	45	theme	hydrated	1368:1375	arg1	gel					1377:1379	a highly hydrated gel	1359:1379	a highly hydrated gel which might contribute to regulate grain hydration	1359:1430	Our results suggest that arabinoxylans are synthesized by the nucellar epidermis, released in the cavity where they form a highly hydrated gel which might contribute to regulate grain hydration.
33775355	2	46	theme	main	170:173	arg1	polysaccharides					210:224	polysaccharides	210:224	polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans)	210:309	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	2	46	theme	main	170:173	arg1	determinants					183:194	The main quality determinants	166:194	The main quality determinants of grains	166:204	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	2	47	theme	starch	234:239	arg1	fibers					258:263	starch but also dietary fibers	234:263	mainly starch but also dietary fibers such as arabinoxylans	227:285	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	2	47	theme	starch	234:239	arg1	arabinoxylans					273:285	arabinoxylans	273:285	arabinoxylans	273:285	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	2	47	theme	starch	234:239	arg1	glucans					302:308	mixed-linkage glucans	288:308	mixed-linkage glucans	288:308	The main quality determinants of grains are polysaccharides (mainly starch but also dietary fibers such as arabinoxylans, mixed-linkage glucans) and proteins synthesized and accumulated during grain development in a specialized storage tissue: the endosperm.
33775355	4	48	theme	grain	690:694	arg1	filling					696:702	grain filling	690:702	grain filling	690:702	This structure is contained in the endosperm cavity where water and nutrients are transferred to support grain filling.
33775355	0	49	theme	hydrated	55:62	arg1	gel					64:66	a highly hydrated gel	46:66	a highly hydrated gel of arabinoxylan	46:82	The endosperm cavity of wheat grains contains a highly hydrated gel of arabinoxylan.
33775355	5	50	theme	wheat	724:728	arg1	development					736:746	the wheat grain development	720:746	the wheat grain development	720:746	While studying the wheat grain development, the cavity content was found to autofluoresce under UV light excitation.
33775355	8	51	theme	resonance	1178:1186	arg1	imaging					1188:1194	Microscopic magnetic resonance imaging	1157:1194	Microscopic magnetic resonance imaging	1157:1194	Microscopic magnetic resonance imaging revealed that the gel is highly hydrated.
33775355	6	52	theme	hydroxycinnamic	1007:1021	arg1	acids					1023:1027	hydroxycinnamic acids	1007:1027	hydroxycinnamic acids	1007:1027	Combining multispectral analysis, Fourier-Transform infrared spectroscopy, immunolabeling and laser-dissection coupled with wet chemistry, we identified in the cavity arabinoxylans and hydroxycinnamic acids.
32471515	4	0	theme	high-resolution	793:807	arg1	sequences					853:861	high-resolution clinical standard morphological and dGEMRIC sequences	793:861	high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil	793:947	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	2	1	theme	gadolinium-enhanced	328:346	arg1	resonance					357:365	gadolinium-enhanced magnetic resonance	328:365	delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC)	320:396	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	4	2	theme	standard	818:825	arg1	sequences					853:861	high-resolution clinical standard morphological and dGEMRIC sequences	793:861	high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil	793:947	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	12	3	theme	imaging	2057:2063	arg1	biomarker					2065:2073	an imaging biomarker	2054:2073	an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA	2054:2160	Beyond morphology, advanced MRI techniques may be used to assess cartilage composition in PsA and to identify early changes in the cartilage as an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA.
32471515	12	3	theme	imaging	2057:2063	arg1	changes					2026:2032	early changes	2020:2032	early changes in the cartilage	2020:2049	Beyond morphology, advanced MRI techniques may be used to assess cartilage composition in PsA and to identify early changes in the cartilage as an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA.
32471515	1	4	from	role	281:284	arg1	pathogenesis					293:304	the pathogenesis	289:304	the pathogenesis of PsA	289:311	BACKGROUND Even though cartilage loss is a known feature of psoriatic arthritis (PsA), little is known about its role in the pathogenesis of PsA.
32471515	12	5	from	application	2090:2100	arg1	monitoring					2116:2125	monitoring	2116:2125	monitoring	2116:2125	Beyond morphology, advanced MRI techniques may be used to assess cartilage composition in PsA and to identify early changes in the cartilage as an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA.
32471515	12	5	from	application	2090:2100	arg1	prediction					2132:2141	prediction	2132:2141	prediction	2132:2141	Beyond morphology, advanced MRI techniques may be used to assess cartilage composition in PsA and to identify early changes in the cartilage as an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA.
32471515	12	5	from	application	2090:2100	arg1	detection					2105:2113	detection	2105:2113	detection	2105:2113	Beyond morphology, advanced MRI techniques may be used to assess cartilage composition in PsA and to identify early changes in the cartilage as an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA.
32471515	1	6	theme	cartilage	191:199	arg1	loss					201:204	cartilage loss	191:204	cartilage loss	191:204	BACKGROUND Even though cartilage loss is a known feature of psoriatic arthritis (PsA), little is known about its role in the pathogenesis of PsA.
32471515	1	6	theme	cartilage	191:199	arg1	feature					217:223	a known feature	209:223	a known feature	209:223	BACKGROUND Even though cartilage loss is a known feature of psoriatic arthritis (PsA), little is known about its role in the pathogenesis of PsA.
32471515	9	7	theme	dGEMRIC	1564:1570	arg1	indices					1572:1578	dGEMRIC indices	1564:1578	dGEMRIC indices	1564:1578	No significant correlations were determined between dGEMRIC indices and bone erosion, bone edema, or bone proliferation.
32471515	2	8	theme	rheumatoid	548:557	arg1	RA					570:571	RA	570:571	RA	570:571	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	2	8	theme	rheumatoid	548:557	arg1	arthritis					559:567	rheumatoid arthritis	548:567	rheumatoid arthritis (RA)	548:572	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	12	9	used	used	1960:1963	arg2	techniques					1942:1951	advanced MRI techniques	1929:1951	advanced MRI techniques	1929:1951	Beyond morphology, advanced MRI techniques may be used to assess cartilage composition in PsA and to identify early changes in the cartilage as an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA.
32471515	0	10	theme	compositional	120:132	arg1	resonance					143:151	psoriatic arthritis-a compositional magnetic resonance	98:151	psoriatic arthritis-a compositional magnetic resonance imaging study	98:165	Proteoglycan loss in the articular cartilage is associated with severity of joint inflammation in psoriatic arthritis-a compositional magnetic resonance imaging study.
32471515	1	11	theme	known	211:215	arg1	loss					201:204	cartilage loss	191:204	cartilage loss	191:204	BACKGROUND Even though cartilage loss is a known feature of psoriatic arthritis (PsA), little is known about its role in the pathogenesis of PsA.
32471515	1	11	theme	known	211:215	arg1	feature					217:223	a known feature	209:223	a known feature	209:223	BACKGROUND Even though cartilage loss is a known feature of psoriatic arthritis (PsA), little is known about its role in the pathogenesis of PsA.
32471515	4	12	theme	MRI	874:876	arg1	Siemens					903:909	Siemens	903:909	Siemens	903:909	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	4	12	theme	MRI	874:876	arg1	scanner					878:884	a 3T MRI scanner	869:884	a 3T MRI scanner (Magnetom Skyra, Siemens)	869:910	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	12	13	theme	cartilage	1975:1983	arg1	composition					1985:1995	cartilage composition	1975:1995	cartilage composition in PsA	1975:2002	Beyond morphology, advanced MRI techniques may be used to assess cartilage composition in PsA and to identify early changes in the cartilage as an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA.
32471515	5	14	theme	dGEMRIC	1001:1007	arg1	indices					1009:1015	dGEMRIC indices	1001:1015	dGEMRIC indices	1001:1015	Images were analyzed by two independent raters for dGEMRIC indices, PsA MRI scores (PsAMRIS), and total cartilage thickness (TCT).
32471515	11	15	dep	in	1859:1860	arg1	vivo					1862:1865	vivo	1862:1865	vivo	1862:1865	Thereby, these findings contribute to in vivo concepts of the disease's pathophysiology.
32471515	9	16	theme	bone	1598:1601	arg1	edema					1603:1607	bone edema	1598:1607	bone edema	1598:1607	No significant correlations were determined between dGEMRIC indices and bone erosion, bone edema, or bone proliferation.
32471515	12	17	theme	PsA	2158:2160	arg1	outcomes					2146:2153	outcomes	2146:2153	outcomes of PsA	2146:2160	Beyond morphology, advanced MRI techniques may be used to assess cartilage composition in PsA and to identify early changes in the cartilage as an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA.
32471515	4	18	theme	proximal	672:679	arg1	PIP					698:700	PIP	698:700	PIP	698:700	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	4	18	theme	proximal	672:679	arg1	interphalangeal					681:695	proximal interphalangeal	672:695	proximal interphalangeal (PIP)	672:701	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	4	18	theme	proximal	672:679	arg1	METHODS					633:639	METHODS	633:639	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA	633:773	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	0	19	from	resonance	143:151	arg1	severity					64:71	severity	64:71	severity of joint inflammation in psoriatic arthritis-a compositional magnetic resonance imaging study	64:165	Proteoglycan loss in the articular cartilage is associated with severity of joint inflammation in psoriatic arthritis-a compositional magnetic resonance imaging study.
32471515	11	20	theme	in	1859:1860	arg1	concepts					1867:1874	in vivo concepts	1859:1874	in vivo concepts of the disease's pathophysiology	1859:1907	Thereby, these findings contribute to in vivo concepts of the disease's pathophysiology.
32471515	9	21	theme	bone	1613:1616	arg1	proliferation					1618:1630	bone proliferation	1613:1630	bone proliferation	1613:1630	No significant correlations were determined between dGEMRIC indices and bone erosion, bone edema, or bone proliferation.
32471515	0	22	from	inflammation	82:93	arg1	resonance					143:151	psoriatic arthritis-a compositional magnetic resonance	98:151	psoriatic arthritis-a compositional magnetic resonance imaging study	98:165	Proteoglycan loss in the articular cartilage is associated with severity of joint inflammation in psoriatic arthritis-a compositional magnetic resonance imaging study.
32471515	6	23	theme	tau	1089:1091	arg1	τ					1119:1119	τ	1119:1119	τ	1119:1119	Kendall tau correlation coefficients (τ) were calculated.
32471515	6	23	theme	tau	1089:1091	arg1	coefficients					1105:1116	Kendall tau correlation coefficients	1081:1116	Kendall tau correlation coefficients (τ)	1081:1120	Kendall tau correlation coefficients (τ) were calculated.
32471515	1	24	theme	PsA	309:311	arg1	pathogenesis					293:304	the pathogenesis	289:304	the pathogenesis of PsA	289:311	BACKGROUND Even though cartilage loss is a known feature of psoriatic arthritis (PsA), little is known about its role in the pathogenesis of PsA.
32471515	2	25	theme	early	466:470	arg1	changes					498:504	such early (i.e., pre-morphological) changes	461:504	such early (i.e., pre-morphological) changes	461:504	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	10	26	dep	CONCLUSION	1633:1642	arg1	PsA					1647:1649	PsA	1647:1649	PsA	1647:1649	CONCLUSION In PsA, proteoglycan loss as assessed by dGEMRIC is associated with periarticular inflammation, synovitis, and flexor tenosynovitis, but not with bone erosion or proliferation.
32471515	12	27	with	biomarker	2065:2073	arg1	application					2090:2100	potential application	2080:2100	potential application in detection, monitoring, and prediction of outcomes of PsA	2080:2160	Beyond morphology, advanced MRI techniques may be used to assess cartilage composition in PsA and to identify early changes in the cartilage as an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA.
32471515	12	28	theme	outcomes	2146:2153	arg1	monitoring					2116:2125	monitoring	2116:2125	monitoring	2116:2125	Beyond morphology, advanced MRI techniques may be used to assess cartilage composition in PsA and to identify early changes in the cartilage as an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA.
32471515	12	28	theme	outcomes	2146:2153	arg1	prediction					2132:2141	prediction	2132:2141	prediction	2132:2141	Beyond morphology, advanced MRI techniques may be used to assess cartilage composition in PsA and to identify early changes in the cartilage as an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA.
32471515	12	28	theme	outcomes	2146:2153	arg1	detection					2105:2113	detection	2105:2113	detection	2105:2113	Beyond morphology, advanced MRI techniques may be used to assess cartilage composition in PsA and to identify early changes in the cartilage as an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA.
32471515	0	29	theme	inflammation	82:93	arg1	severity					64:71	severity	64:71	severity of joint inflammation in psoriatic arthritis-a compositional magnetic resonance imaging study	64:165	Proteoglycan loss in the articular cartilage is associated with severity of joint inflammation in psoriatic arthritis-a compositional magnetic resonance imaging study.
32471515	8	30	theme	Significant	1392:1402	arg1	correlations					1413:1424	Significant positive correlations	1392:1424	Significant positive correlations	1392:1424	Significant positive correlations were found between TCT and dGEMRIC indices at all joint levels (τ = 0.43, p < 0.001).
32471515	4	31	theme	interphalangeal	715:729	arg1	joints					737:742	distal interphalangeal (DIP) joints	708:742	distal interphalangeal (DIP) joints	708:742	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	0	32	theme	psoriatic	98:106	arg1	resonance					143:151	psoriatic arthritis-a compositional magnetic resonance	98:151	psoriatic arthritis-a compositional magnetic resonance imaging study	98:165	Proteoglycan loss in the articular cartilage is associated with severity of joint inflammation in psoriatic arthritis-a compositional magnetic resonance imaging study.
32471515	5	33	theme	MRI	1022:1024	arg1	PsAMRIS					1034:1040	PsAMRIS	1034:1040	PsAMRIS	1034:1040	Images were analyzed by two independent raters for dGEMRIC indices, PsA MRI scores (PsAMRIS), and total cartilage thickness (TCT).
32471515	5	33	theme	MRI	1022:1024	arg1	scores					1026:1031	PsA MRI scores	1018:1031	PsA MRI scores (PsAMRIS)	1018:1041	Images were analyzed by two independent raters for dGEMRIC indices, PsA MRI scores (PsAMRIS), and total cartilage thickness (TCT).
32471515	4	34	dep	METHODS	633:639	arg1	PIP					698:700	PIP	698:700	PIP	698:700	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	4	34	dep	METHODS	633:639	arg1	interphalangeal					681:695	proximal interphalangeal	672:695	proximal interphalangeal (PIP)	672:701	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	4	34	dep	METHODS	633:639	arg1	joints					737:742	distal interphalangeal (DIP) joints	708:742	distal interphalangeal (DIP) joints	708:742	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	4	34	dep	METHODS	633:639	arg1	METHODS					633:639	METHODS	633:639	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA	633:773	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	4	34	dep	METHODS	633:639	arg1	MCP					666:668	The metacarpophalangeal (MCP)	641:669	The metacarpophalangeal (MCP)	641:669	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	1	35	dep	BACKGROUND	168:177	arg1	loss					201:204	cartilage loss	191:204	cartilage loss	191:204	BACKGROUND Even though cartilage loss is a known feature of psoriatic arthritis (PsA), little is known about its role in the pathogenesis of PsA.
32471515	1	35	dep	BACKGROUND	168:177	arg1	feature					217:223	a known feature	209:223	a known feature	209:223	BACKGROUND Even though cartilage loss is a known feature of psoriatic arthritis (PsA), little is known about its role in the pathogenesis of PsA.
32471515	2	36	theme	cartilage	378:386	arg1	marker					416:421	a non-invasive marker	401:421	a non-invasive marker of the tissue's proteoglycan content	401:458	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	2	36	theme	cartilage	378:386	arg1	imaging					367:373	delayed gadolinium-enhanced magnetic resonance imaging	320:373	delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC)	320:396	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	0	37	theme	Proteoglycan	0:11	arg1	loss					13:16	Proteoglycan loss	0:16	Proteoglycan loss in the articular cartilage	0:43	Proteoglycan loss in the articular cartilage is associated with severity of joint inflammation in psoriatic arthritis-a compositional magnetic resonance imaging study.
32471515	2	38	theme	non-invasive	403:414	arg1	marker					416:421	a non-invasive marker	401:421	a non-invasive marker of the tissue's proteoglycan content	401:458	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	2	38	theme	non-invasive	403:414	arg1	imaging					367:373	delayed gadolinium-enhanced magnetic resonance imaging	320:373	delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC)	320:396	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	4	39	theme	dedicated	918:926	arg1	coil					944:947	a dedicated 16-channel hand coil	916:947	a dedicated 16-channel hand coil	916:947	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	8	40	theme	TCT	1445:1447	arg1	indices					1461:1467	TCT and dGEMRIC indices	1445:1467	TCT and dGEMRIC indices	1445:1467	Significant positive correlations were found between TCT and dGEMRIC indices at all joint levels (τ = 0.43, p < 0.001).
32471515	13	41	dep	December	2194:2201	arg1	REGISTRATION					2169:2180	TRIAL REGISTRATION 2014123117	2163:2191	TRIAL REGISTRATION 2014123117	2163:2191	TRIAL REGISTRATION 2014123117, December 2014.
32471515	0	42	theme	articular	25:33	arg1	cartilage					35:43	the articular cartilage	21:43	the articular cartilage	21:43	Proteoglycan loss in the articular cartilage is associated with severity of joint inflammation in psoriatic arthritis-a compositional magnetic resonance imaging study.
32471515	8	43	located	found	1431:1435	arg2	correlations					1413:1424	Significant positive correlations	1392:1424	Significant positive correlations	1392:1424	Significant positive correlations were found between TCT and dGEMRIC indices at all joint levels (τ = 0.43, p < 0.001).
32471515	8	43	located	found	1431:1435	arg1	levels					1482:1487	all joint levels	1472:1487	all joint levels (τ = 0.43, p < 0.001)	1472:1509	Significant positive correlations were found between TCT and dGEMRIC indices at all joint levels (τ = 0.43, p < 0.001).
32471515	7	44	theme	dGEMRIC	1198:1204	arg1	indices					1206:1212	dGEMRIC indices	1198:1212	dGEMRIC indices	1198:1212	RESULTS We found significant negative correlations between dGEMRIC indices and total PsAMRIS (τ = - 0.5, p = 0.012), synovitis (τ = - 0.56, p = 0.006), flexor tenosynovitis (τ = - 0.4, p = 0.049), and periarticular inflammation (τ = - 0.72, p < 0.001).
32471515	2	45	theme	proteoglycan	439:450	arg1	content					452:458	the tissue's proteoglycan content	426:458	the tissue's proteoglycan content	426:458	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	8	46	theme	dGEMRIC	1453:1459	arg1	indices					1461:1467	TCT and dGEMRIC indices	1445:1467	TCT and dGEMRIC indices	1445:1467	Significant positive correlations were found between TCT and dGEMRIC indices at all joint levels (τ = 0.43, p < 0.001).
32471515	10	47	theme	periarticular	1712:1724	arg1	inflammation					1726:1737	periarticular inflammation	1712:1737	periarticular inflammation	1712:1737	CONCLUSION In PsA, proteoglycan loss as assessed by dGEMRIC is associated with periarticular inflammation, synovitis, and flexor tenosynovitis, but not with bone erosion or proliferation.
32471515	2	48	theme	delayed	320:326	arg1	marker					416:421	a non-invasive marker	401:421	a non-invasive marker of the tissue's proteoglycan content	401:458	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	2	48	theme	delayed	320:326	arg1	imaging					367:373	delayed gadolinium-enhanced magnetic resonance imaging	320:373	delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC)	320:396	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	5	49	theme	cartilage	1054:1062	arg1	TCT					1075:1077	TCT	1075:1077	TCT	1075:1077	Images were analyzed by two independent raters for dGEMRIC indices, PsA MRI scores (PsAMRIS), and total cartilage thickness (TCT).
32471515	5	49	theme	cartilage	1054:1062	arg1	thickness					1064:1072	total cartilage thickness	1048:1072	total cartilage thickness (TCT)	1048:1078	Images were analyzed by two independent raters for dGEMRIC indices, PsA MRI scores (PsAMRIS), and total cartilage thickness (TCT).
32471515	10	50	theme	flexor	1755:1760	arg1	tenosynovitis					1762:1774	flexor tenosynovitis	1755:1774	flexor tenosynovitis	1755:1774	CONCLUSION In PsA, proteoglycan loss as assessed by dGEMRIC is associated with periarticular inflammation, synovitis, and flexor tenosynovitis, but not with bone erosion or proliferation.
32471515	4	51	theme	DIP	732:734	arg1	joints					737:742	distal interphalangeal (DIP) joints	708:742	distal interphalangeal (DIP) joints	708:742	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	1	52	theme	psoriatic	228:236	arg1	PsA					249:251	PsA	249:251	PsA	249:251	BACKGROUND Even though cartilage loss is a known feature of psoriatic arthritis (PsA), little is known about its role in the pathogenesis of PsA.
32471515	1	52	theme	psoriatic	228:236	arg1	arthritis					238:246	psoriatic arthritis	228:246	psoriatic arthritis (PsA)	228:252	BACKGROUND Even though cartilage loss is a known feature of psoriatic arthritis (PsA), little is known about its role in the pathogenesis of PsA.
32471515	7	53	theme	significant	1156:1166	arg1	correlations					1177:1188	significant negative correlations	1156:1188	significant negative correlations between dGEMRIC indices and total PsAMRIS	1156:1230	RESULTS We found significant negative correlations between dGEMRIC indices and total PsAMRIS (τ = - 0.5, p = 0.012), synovitis (τ = - 0.56, p = 0.006), flexor tenosynovitis (τ = - 0.4, p = 0.049), and periarticular inflammation (τ = - 0.72, p < 0.001).
32471515	8	54	theme	joint	1476:1480	arg1	levels					1482:1487	all joint levels	1472:1487	all joint levels (τ = 0.43, p < 0.001)	1472:1509	Significant positive correlations were found between TCT and dGEMRIC indices at all joint levels (τ = 0.43, p < 0.001).
32471515	4	55	theme	hand	939:942	arg1	coil					944:947	a dedicated 16-channel hand coil	916:947	a dedicated 16-channel hand coil	916:947	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	2	56	theme	magnetic	348:355	arg1	resonance					357:365	gadolinium-enhanced magnetic resonance	328:365	delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC)	320:396	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	7	57	theme	periarticular	1340:1352	arg1	inflammation					1354:1365	periarticular inflammation	1340:1365	periarticular inflammation (τ = - 0.72, p < 0.001)	1340:1389	RESULTS We found significant negative correlations between dGEMRIC indices and total PsAMRIS (τ = - 0.5, p = 0.012), synovitis (τ = - 0.56, p = 0.006), flexor tenosynovitis (τ = - 0.4, p = 0.049), and periarticular inflammation (τ = - 0.72, p < 0.001).
32471515	9	58	theme	significant	1515:1525	arg1	correlations					1527:1538	No significant correlations	1512:1538	No significant correlations	1512:1538	No significant correlations were determined between dGEMRIC indices and bone erosion, bone edema, or bone proliferation.
32471515	2	59	from	inflammation	532:543	arg1	RA					570:571	RA	570:571	RA	570:571	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	2	59	from	inflammation	532:543	arg1	arthritis					559:567	rheumatoid arthritis	548:567	rheumatoid arthritis (RA)	548:572	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	1	60	theme	little	255:260	arg1	loss					201:204	cartilage loss	191:204	cartilage loss	191:204	BACKGROUND Even though cartilage loss is a known feature of psoriatic arthritis (PsA), little is known about its role in the pathogenesis of PsA.
32471515	1	60	theme	little	255:260	arg1	feature					217:223	a known feature	209:223	a known feature	209:223	BACKGROUND Even though cartilage loss is a known feature of psoriatic arthritis (PsA), little is known about its role in the pathogenesis of PsA.
32471515	4	61	theme	clinical	809:816	arg1	sequences					853:861	high-resolution clinical standard morphological and dGEMRIC sequences	793:861	high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil	793:947	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	12	62	theme	advanced	1929:1936	arg1	techniques					1942:1951	advanced MRI techniques	1929:1951	advanced MRI techniques	1929:1951	Beyond morphology, advanced MRI techniques may be used to assess cartilage composition in PsA and to identify early changes in the cartilage as an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA.
32471515	7	63	dep	synovitis	1256:1264	arg1	p = 0.006					1279:1287	p = 0.006	1279:1287	p = 0.006	1279:1287	RESULTS We found significant negative correlations between dGEMRIC indices and total PsAMRIS (τ = - 0.5, p = 0.012), synovitis (τ = - 0.56, p = 0.006), flexor tenosynovitis (τ = - 0.4, p = 0.049), and periarticular inflammation (τ = - 0.72, p < 0.001).
32471515	4	64	theme	active	764:769	arg1	PsA					771:773	active PsA	764:773	active PsA	764:773	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	10	65	theme	bone	1790:1793	arg1	erosion					1795:1801	bone erosion	1790:1801	bone erosion	1790:1801	CONCLUSION In PsA, proteoglycan loss as assessed by dGEMRIC is associated with periarticular inflammation, synovitis, and flexor tenosynovitis, but not with bone erosion or proliferation.
32471515	10	66	theme	proteoglycan	1652:1663	arg1	loss					1665:1668	proteoglycan loss	1652:1668	proteoglycan loss as assessed by dGEMRIC	1652:1691	CONCLUSION In PsA, proteoglycan loss as assessed by dGEMRIC is associated with periarticular inflammation, synovitis, and flexor tenosynovitis, but not with bone erosion or proliferation.
32471515	7	67	theme	flexor	1291:1296	arg1	tenosynovitis					1298:1310	flexor tenosynovitis	1291:1310	flexor tenosynovitis (τ = - 0.4, p = 0.049)	1291:1333	RESULTS We found significant negative correlations between dGEMRIC indices and total PsAMRIS (τ = - 0.5, p = 0.012), synovitis (τ = - 0.56, p = 0.006), flexor tenosynovitis (τ = - 0.4, p = 0.049), and periarticular inflammation (τ = - 0.72, p < 0.001).
32471515	0	68	theme	magnetic	134:141	arg1	resonance					143:151	psoriatic arthritis-a compositional magnetic resonance	98:151	psoriatic arthritis-a compositional magnetic resonance imaging study	98:165	Proteoglycan loss in the articular cartilage is associated with severity of joint inflammation in psoriatic arthritis-a compositional magnetic resonance imaging study.
32471515	9	69	theme	bone	1584:1587	arg1	erosion					1589:1595	bone erosion	1584:1595	bone erosion	1584:1595	No significant correlations were determined between dGEMRIC indices and bone erosion, bone edema, or bone proliferation.
32471515	10	70	dep	loss	1665:1668	arg1	assessed					1673:1680	assessed	1673:1680	assessed by dGEMRIC	1673:1691	CONCLUSION In PsA, proteoglycan loss as assessed by dGEMRIC is associated with periarticular inflammation, synovitis, and flexor tenosynovitis, but not with bone erosion or proliferation.
32471515	13	71	theme	TRIAL	2163:2167	arg1	REGISTRATION					2169:2180	TRIAL REGISTRATION 2014123117	2163:2191	TRIAL REGISTRATION 2014123117	2163:2191	TRIAL REGISTRATION 2014123117, December 2014.
32471515	4	72	theme	3T	871:872	arg1	Siemens					903:909	Siemens	903:909	Siemens	903:909	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	4	72	theme	3T	871:872	arg1	scanner					878:884	a 3T MRI scanner	869:884	a 3T MRI scanner (Magnetom Skyra, Siemens)	869:910	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	0	73	from	severity	64:71	arg1	resonance					143:151	psoriatic arthritis-a compositional magnetic resonance	98:151	psoriatic arthritis-a compositional magnetic resonance imaging study	98:165	Proteoglycan loss in the articular cartilage is associated with severity of joint inflammation in psoriatic arthritis-a compositional magnetic resonance imaging study.
32471515	7	74	dep	tenosynovitis	1298:1310	arg1	p = 0.049					1324:1332	p = 0.049	1324:1332	p = 0.049	1324:1332	RESULTS We found significant negative correlations between dGEMRIC indices and total PsAMRIS (τ = - 0.5, p = 0.012), synovitis (τ = - 0.56, p = 0.006), flexor tenosynovitis (τ = - 0.4, p = 0.049), and periarticular inflammation (τ = - 0.72, p < 0.001).
32471515	4	75	theme	morphological	827:839	arg1	sequences					853:861	high-resolution clinical standard morphological and dGEMRIC sequences	793:861	high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil	793:947	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	12	76	theme	MRI	1938:1940	arg1	techniques					1942:1951	advanced MRI techniques	1929:1951	advanced MRI techniques	1929:1951	Beyond morphology, advanced MRI techniques may be used to assess cartilage composition in PsA and to identify early changes in the cartilage as an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA.
32471515	4	77	theme	dGEMRIC	845:851	arg1	sequences					853:861	high-resolution clinical standard morphological and dGEMRIC sequences	793:861	high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil	793:947	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	6	78	theme	correlation	1093:1103	arg1	τ					1119:1119	τ	1119:1119	τ	1119:1119	Kendall tau correlation coefficients (τ) were calculated.
32471515	6	78	theme	correlation	1093:1103	arg1	coefficients					1105:1116	Kendall tau correlation coefficients	1081:1116	Kendall tau correlation coefficients (τ)	1081:1120	Kendall tau correlation coefficients (τ) were calculated.
32471515	4	79	with	patients	750:757	arg1	PsA					771:773	active PsA	764:773	active PsA	764:773	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	5	80	theme	independent	978:988	arg1	raters					990:995	two independent raters	974:995	two independent raters for dGEMRIC indices, PsA MRI scores (PsAMRIS), and total cartilage thickness (TCT)	974:1078	Images were analyzed by two independent raters for dGEMRIC indices, PsA MRI scores (PsAMRIS), and total cartilage thickness (TCT).
32471515	6	81	theme	Kendall	1081:1087	arg1	τ					1119:1119	τ	1119:1119	τ	1119:1119	Kendall tau correlation coefficients (τ) were calculated.
32471515	6	81	theme	Kendall	1081:1087	arg1	coefficients					1105:1116	Kendall tau correlation coefficients	1081:1116	Kendall tau correlation coefficients (τ)	1081:1120	Kendall tau correlation coefficients (τ) were calculated.
32471515	0	82	theme	joint	76:80	arg1	inflammation					82:93	joint inflammation	76:93	joint inflammation in psoriatic arthritis-a compositional magnetic resonance imaging study	76:165	Proteoglycan loss in the articular cartilage is associated with severity of joint inflammation in psoriatic arthritis-a compositional magnetic resonance imaging study.
32471515	2	83	theme	such	461:464	arg1	changes					498:504	such early (i.e., pre-morphological) changes	461:504	such early (i.e., pre-morphological) changes	461:504	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	0	84	from	loss	13:16	arg1	cartilage					35:43	the articular cartilage	21:43	the articular cartilage	21:43	Proteoglycan loss in the articular cartilage is associated with severity of joint inflammation in psoriatic arthritis-a compositional magnetic resonance imaging study.
32471515	4	85	theme	metacarpophalangeal	645:663	arg1	METHODS					633:639	METHODS	633:639	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA	633:773	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	4	85	theme	metacarpophalangeal	645:663	arg1	MCP					666:668	The metacarpophalangeal (MCP)	641:669	The metacarpophalangeal (MCP)	641:669	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	8	86	theme	positive	1404:1411	arg1	correlations					1413:1424	Significant positive correlations	1392:1424	Significant positive correlations	1392:1424	Significant positive correlations were found between TCT and dGEMRIC indices at all joint levels (τ = 0.43, p < 0.001).
32471515	12	87	theme	potential	2080:2088	arg1	application					2090:2100	potential application	2080:2100	potential application in detection, monitoring, and prediction of outcomes of PsA	2080:2160	Beyond morphology, advanced MRI techniques may be used to assess cartilage composition in PsA and to identify early changes in the cartilage as an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA.
32471515	0	88	theme	arthritis-a	108:118	arg1	resonance					143:151	psoriatic arthritis-a compositional magnetic resonance	98:151	psoriatic arthritis-a compositional magnetic resonance imaging study	98:165	Proteoglycan loss in the articular cartilage is associated with severity of joint inflammation in psoriatic arthritis-a compositional magnetic resonance imaging study.
32471515	5	89	theme	PsA	1018:1020	arg1	PsAMRIS					1034:1040	PsAMRIS	1034:1040	PsAMRIS	1034:1040	Images were analyzed by two independent raters for dGEMRIC indices, PsA MRI scores (PsAMRIS), and total cartilage thickness (TCT).
32471515	5	89	theme	PsA	1018:1020	arg1	scores					1026:1031	PsA MRI scores	1018:1031	PsA MRI scores (PsAMRIS)	1018:1041	Images were analyzed by two independent raters for dGEMRIC indices, PsA MRI scores (PsAMRIS), and total cartilage thickness (TCT).
32471515	12	90	from	composition	1985:1995	arg1	PsA					2000:2002	PsA	2000:2002	PsA	2000:2002	Beyond morphology, advanced MRI techniques may be used to assess cartilage composition in PsA and to identify early changes in the cartilage as an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA.
32471515	4	91	theme	distal	708:713	arg1	joints					737:742	distal interphalangeal (DIP) joints	708:742	distal interphalangeal (DIP) joints	708:742	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	7	92	theme	total	1218:1222	arg1	PsAMRIS					1224:1230	total PsAMRIS	1218:1230	total PsAMRIS	1218:1230	RESULTS We found significant negative correlations between dGEMRIC indices and total PsAMRIS (τ = - 0.5, p = 0.012), synovitis (τ = - 0.56, p = 0.006), flexor tenosynovitis (τ = - 0.4, p = 0.049), and periarticular inflammation (τ = - 0.72, p < 0.001).
32471515	7	93	dep	inflammation	1354:1365	arg1	p < 0.001					1380:1388	p < 0.001	1380:1388	p < 0.001	1380:1388	RESULTS We found significant negative correlations between dGEMRIC indices and total PsAMRIS (τ = - 0.5, p = 0.012), synovitis (τ = - 0.56, p = 0.006), flexor tenosynovitis (τ = - 0.4, p = 0.049), and periarticular inflammation (τ = - 0.72, p < 0.001).
32471515	2	94	dep	pre-morphological	479:495	arg1	i.e.					473:476	i.e.	473:476	i.e.	473:476	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	7	95	dep	RESULTS	1139:1145	arg1	found					1150:1154	found	1150:1154	found significant negative correlations between dGEMRIC indices and total PsAMRIS (τ = - 0.5, p = 0.012), synovitis (τ = - 0.56, p = 0.006), flexor tenosynovitis (τ = - 0.4, p = 0.049), and periarticular inflammation (τ = - 0.72, p < 0.001)	1150:1389	RESULTS We found significant negative correlations between dGEMRIC indices and total PsAMRIS (τ = - 0.5, p = 0.012), synovitis (τ = - 0.56, p = 0.006), flexor tenosynovitis (τ = - 0.4, p = 0.049), and periarticular inflammation (τ = - 0.72, p < 0.001).
32471515	2	96	theme	content	452:458	arg1	marker					416:421	a non-invasive marker	401:421	a non-invasive marker of the tissue's proteoglycan content	401:458	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	2	96	theme	content	452:458	arg1	imaging					367:373	delayed gadolinium-enhanced magnetic resonance imaging	320:373	delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC)	320:396	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	5	97	theme	total	1048:1052	arg1	TCT					1075:1077	TCT	1075:1077	TCT	1075:1077	Images were analyzed by two independent raters for dGEMRIC indices, PsA MRI scores (PsAMRIS), and total cartilage thickness (TCT).
32471515	5	97	theme	total	1048:1052	arg1	thickness					1064:1072	total cartilage thickness	1048:1072	total cartilage thickness (TCT)	1048:1078	Images were analyzed by two independent raters for dGEMRIC indices, PsA MRI scores (PsAMRIS), and total cartilage thickness (TCT).
32471515	2	98	dep	early	466:470	arg1	pre-morphological					479:495	pre-morphological	479:495	pre-morphological	479:495	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	8	99	dep	levels	1482:1487	arg1	τ = 0.43					1490:1497	τ = 0.43	1490:1497	τ = 0.43	1490:1497	Significant positive correlations were found between TCT and dGEMRIC indices at all joint levels (τ = 0.43, p < 0.001).
32471515	7	100	theme	negative	1168:1175	arg1	correlations					1177:1188	significant negative correlations	1156:1188	significant negative correlations between dGEMRIC indices and total PsAMRIS	1156:1230	RESULTS We found significant negative correlations between dGEMRIC indices and total PsAMRIS (τ = - 0.5, p = 0.012), synovitis (τ = - 0.56, p = 0.006), flexor tenosynovitis (τ = - 0.4, p = 0.049), and periarticular inflammation (τ = - 0.72, p < 0.001).
32471515	4	101	theme	16-channel	928:937	arg1	coil					944:947	a dedicated 16-channel hand coil	916:947	a dedicated 16-channel hand coil	916:947	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	4	102	theme	patients	750:757	arg1	METHODS					633:639	METHODS	633:639	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA	633:773	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	4	102	theme	patients	750:757	arg1	interphalangeal					681:695	proximal interphalangeal	672:695	proximal interphalangeal (PIP)	672:701	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	4	102	theme	patients	750:757	arg1	MCP					666:668	The metacarpophalangeal (MCP)	641:669	The metacarpophalangeal (MCP)	641:669	METHODS The metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints of 17 patients with active PsA were evaluated by high-resolution clinical standard morphological and dGEMRIC sequences using a 3T MRI scanner (Magnetom Skyra, Siemens) and a dedicated 16-channel hand coil.
32471515	1	103	theme	arthritis	238:246	arg1	loss					201:204	cartilage loss	191:204	cartilage loss	191:204	BACKGROUND Even though cartilage loss is a known feature of psoriatic arthritis (PsA), little is known about its role in the pathogenesis of PsA.
32471515	1	103	theme	arthritis	238:246	arg1	feature					217:223	a known feature	209:223	a known feature	209:223	BACKGROUND Even though cartilage loss is a known feature of psoriatic arthritis (PsA), little is known about its role in the pathogenesis of PsA.
32471515	12	104	theme	early	2020:2024	arg1	biomarker					2065:2073	an imaging biomarker	2054:2073	an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA	2054:2160	Beyond morphology, advanced MRI techniques may be used to assess cartilage composition in PsA and to identify early changes in the cartilage as an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA.
32471515	12	104	theme	early	2020:2024	arg1	changes					2026:2032	early changes	2020:2032	early changes in the cartilage	2020:2049	Beyond morphology, advanced MRI techniques may be used to assess cartilage composition in PsA and to identify early changes in the cartilage as an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA.
32471515	2	105	theme	resonance	357:365	arg1	marker					416:421	a non-invasive marker	401:421	a non-invasive marker of the tissue's proteoglycan content	401:458	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	2	105	theme	resonance	357:365	arg1	imaging					367:373	delayed gadolinium-enhanced magnetic resonance imaging	320:373	delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC)	320:396	Using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) as a non-invasive marker of the tissue's proteoglycan content, such early (i.e., pre-morphological) changes have been associated with inflammation in rheumatoid arthritis (RA).
32471515	12	106	from	changes	2026:2032	arg1	cartilage					2041:2049	the cartilage	2037:2049	the cartilage	2037:2049	Beyond morphology, advanced MRI techniques may be used to assess cartilage composition in PsA and to identify early changes in the cartilage as an imaging biomarker with potential application in detection, monitoring, and prediction of outcomes of PsA.
32057861	3	0	theme	softening	580:588	arg1	loss					517:520	the weight loss	506:520	the weight loss	506:520	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	3	0	theme	softening	580:588	arg1	process					535:541	respiration process	523:541	respiration process	523:541	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	1	1	theme	β-glucan	194:201	arg1	SABG					223:226	SABG	223:226	SABG	223:226	Apples (Rich Red) were coated with wheat straw arabinoxylan (AX) and β-glucan stearic acid ester (SABG) composite coating material in the concentration range of 1-2%.
32057861	1	1	theme	β-glucan	194:201	arg1	ester					216:220	β-glucan stearic acid ester	194:220	β-glucan stearic acid ester (SABG)	194:227	Apples (Rich Red) were coated with wheat straw arabinoxylan (AX) and β-glucan stearic acid ester (SABG) composite coating material in the concentration range of 1-2%.
32057861	0	2	theme	composite	52:60	arg1	coating					62:68	composite coating	52:68	composite coating	52:68	Improved postharvest quality of apple (Rich Red) by composite coating based on arabinoxylan and β-glucan stearic acid ester.
32057861	3	3	theme	color	544:548	arg1	process					535:541	respiration process	523:541	respiration process	523:541	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	3	3	theme	color	544:548	arg1	degradation					550:560	color degradation	544:560	color degradation	544:560	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	1	4	theme	stearic	203:209	arg1	SABG					223:226	SABG	223:226	SABG	223:226	Apples (Rich Red) were coated with wheat straw arabinoxylan (AX) and β-glucan stearic acid ester (SABG) composite coating material in the concentration range of 1-2%.
32057861	1	4	theme	stearic	203:209	arg1	ester					216:220	β-glucan stearic acid ester	194:220	β-glucan stearic acid ester (SABG)	194:227	Apples (Rich Red) were coated with wheat straw arabinoxylan (AX) and β-glucan stearic acid ester (SABG) composite coating material in the concentration range of 1-2%.
32057861	4	5	theme	sensorial	797:805	arg1	attributes					807:816	sensorial attributes	797:816	sensorial attributes	797:816	However, the AX-SABG coatings were found more effective in preventing the aroma loss, reducing microbial spoilage and maintaining sensorial attributes as compared to shellac and uncoated apples during storage.
32057861	3	6	theme	AX-SABG	432:438	arg1	material					475:482	both AX-SABG (1-2%) and shellac (1-2%) coatings material	427:482	both AX-SABG (1-2%) and shellac (1-2%) coatings material	427:482	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	1	7	theme	acid	211:214	arg1	SABG					223:226	SABG	223:226	SABG	223:226	Apples (Rich Red) were coated with wheat straw arabinoxylan (AX) and β-glucan stearic acid ester (SABG) composite coating material in the concentration range of 1-2%.
32057861	1	7	theme	acid	211:214	arg1	ester					216:220	β-glucan stearic acid ester	194:220	β-glucan stearic acid ester (SABG)	194:227	Apples (Rich Red) were coated with wheat straw arabinoxylan (AX) and β-glucan stearic acid ester (SABG) composite coating material in the concentration range of 1-2%.
32057861	1	8	theme	%	289:289	arg1	range					277:281	the concentration range	259:281	the concentration range of 1-2%	259:289	Apples (Rich Red) were coated with wheat straw arabinoxylan (AX) and β-glucan stearic acid ester (SABG) composite coating material in the concentration range of 1-2%.
32057861	1	9	from	material	247:254	arg1	range					277:281	the concentration range	259:281	the concentration range of 1-2%	259:289	Apples (Rich Red) were coated with wheat straw arabinoxylan (AX) and β-glucan stearic acid ester (SABG) composite coating material in the concentration range of 1-2%.
32057861	4	10	theme	uncoated	845:852	arg1	apples					854:859	uncoated apples	845:859	uncoated apples during storage	845:874	However, the AX-SABG coatings were found more effective in preventing the aroma loss, reducing microbial spoilage and maintaining sensorial attributes as compared to shellac and uncoated apples during storage.
32057861	2	11	theme	coated	324:329	arg1	apples					331:336	coated apples	324:336	coated apples	324:336	The postharvest storage life of coated apples was studied at 22 °C (±2) with 65% and 85% relative humidity for 45 days.
32057861	4	12	theme	AX-SABG	680:686	arg1	coatings					688:695	the AX-SABG coatings	676:695	the AX-SABG coatings	676:695	However, the AX-SABG coatings were found more effective in preventing the aroma loss, reducing microbial spoilage and maintaining sensorial attributes as compared to shellac and uncoated apples during storage.
32057861	0	13	theme	postharvest	9:19	arg1	quality					21:27	Improved postharvest quality	0:27	Improved postharvest quality of apple (Rich Red) by composite coating	0:68	Improved postharvest quality of apple (Rich Red) by composite coating based on arabinoxylan and β-glucan stearic acid ester.
32057861	3	14	theme	fruit	574:578	arg1	softening					580:588	fruit softening	574:588	fruit softening	574:588	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	0	15	theme	Improved	0:7	arg1	quality					21:27	Improved postharvest quality	0:27	Improved postharvest quality of apple (Rich Red) by composite coating	0:68	Improved postharvest quality of apple (Rich Red) by composite coating based on arabinoxylan and β-glucan stearic acid ester.
32057861	2	16	theme	storage	308:314	arg1	life					316:319	The postharvest storage life	292:319	The postharvest storage life of coated apples	292:336	The postharvest storage life of coated apples was studied at 22 °C (±2) with 65% and 85% relative humidity for 45 days.
32057861	0	17	theme	stearic	105:111	arg1	acid					113:116	β-glucan stearic acid	96:116	β-glucan stearic acid ester	96:122	Improved postharvest quality of apple (Rich Red) by composite coating based on arabinoxylan and β-glucan stearic acid ester.
32057861	3	18	theme	ripening	594:601	arg1	loss					517:520	the weight loss	506:520	the weight loss	506:520	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	3	18	theme	ripening	594:601	arg1	process					535:541	respiration process	523:541	respiration process	523:541	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	0	19	theme	β-glucan	96:103	arg1	acid					113:116	β-glucan stearic acid	96:116	β-glucan stearic acid ester	96:122	Improved postharvest quality of apple (Rich Red) by composite coating based on arabinoxylan and β-glucan stearic acid ester.
32057861	3	20	theme	weight	510:515	arg1	loss					517:520	the weight loss	506:520	the weight loss	506:520	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	3	20	theme	weight	510:515	arg1	process					535:541	respiration process	523:541	respiration process	523:541	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	1	21	dep	Apples	125:130	arg1	Red					138:140	Red	138:140	Red	138:140	Apples (Rich Red) were coated with wheat straw arabinoxylan (AX) and β-glucan stearic acid ester (SABG) composite coating material in the concentration range of 1-2%.
32057861	1	22	theme	wheat	160:164	arg1	arabinoxylan					172:183	wheat straw arabinoxylan	160:183	wheat straw arabinoxylan (AX)	160:188	Apples (Rich Red) were coated with wheat straw arabinoxylan (AX) and β-glucan stearic acid ester (SABG) composite coating material in the concentration range of 1-2%.
32057861	1	23	theme	composite	229:237	arg1	material					247:254	composite coating material	229:254	composite coating material in the concentration range of 1-2%	229:289	Apples (Rich Red) were coated with wheat straw arabinoxylan (AX) and β-glucan stearic acid ester (SABG) composite coating material in the concentration range of 1-2%.
32057861	1	24	theme	straw	166:170	arg1	arabinoxylan					172:183	wheat straw arabinoxylan	160:183	wheat straw arabinoxylan (AX)	160:188	Apples (Rich Red) were coated with wheat straw arabinoxylan (AX) and β-glucan stearic acid ester (SABG) composite coating material in the concentration range of 1-2%.
32057861	0	25	theme	apple	32:36	arg1	quality					21:27	Improved postharvest quality	0:27	Improved postharvest quality of apple (Rich Red) by composite coating	0:68	Improved postharvest quality of apple (Rich Red) by composite coating based on arabinoxylan and β-glucan stearic acid ester.
32057861	1	26	theme	coating	239:245	arg1	material					247:254	composite coating material	229:254	composite coating material in the concentration range of 1-2%	229:289	Apples (Rich Red) were coated with wheat straw arabinoxylan (AX) and β-glucan stearic acid ester (SABG) composite coating material in the concentration range of 1-2%.
32057861	0	27	theme	acid	113:116	arg1	ester					118:122	β-glucan stearic acid ester	96:122	β-glucan stearic acid ester	96:122	Improved postharvest quality of apple (Rich Red) by composite coating based on arabinoxylan and β-glucan stearic acid ester.
32057861	2	28	theme	apples	331:336	arg1	life					316:319	The postharvest storage life	292:319	The postharvest storage life of coated apples	292:336	The postharvest storage life of coated apples was studied at 22 °C (±2) with 65% and 85% relative humidity for 45 days.
32057861	3	29	theme	uncoated	628:635	arg1	apples					637:642	the uncoated apples	624:642	the uncoated apples up to 30 days storage	624:664	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	2	30	dep	%	379:379	arg1	humidity					390:397	relative humidity	381:397	85% relative humidity for 45 days	377:409	The postharvest storage life of coated apples was studied at 22 °C (±2) with 65% and 85% relative humidity for 45 days.
32057861	3	31	dep	storage	658:664	arg1	up					644:645	up	644:645	up	644:645	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	3	32	theme	shellac	451:457	arg1	coatings					466:473	both AX-SABG (1-2%) and shellac (1-2%) coatings	427:473	coatings	466:473	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	3	32	theme	shellac	451:457	arg1	%					463:463	1-2%	460:463	1-2%	460:463	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	3	33	dep	softening	580:588	arg1	index					603:607	index	603:607	index	603:607	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	4	34	theme	microbial	762:770	arg1	spoilage					772:779	microbial spoilage	762:779	microbial spoilage	762:779	However, the AX-SABG coatings were found more effective in preventing the aroma loss, reducing microbial spoilage and maintaining sensorial attributes as compared to shellac and uncoated apples during storage.
32057861	0	35	dep	apple	32:36	arg1	Red					44:46	Red	44:46	Red	44:46	Improved postharvest quality of apple (Rich Red) by composite coating based on arabinoxylan and β-glucan stearic acid ester.
32057861	3	36	theme	material	475:482	arg1	Application					412:422	Application	412:422	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material	412:482	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	3	37	theme	30 days	650:656	arg1	storage					658:664	30 days storage	650:664	30 days storage	650:664	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	1	38	dep	arabinoxylan	172:183	arg1	material					247:254	composite coating material	229:254	composite coating material in the concentration range of 1-2%	229:289	Apples (Rich Red) were coated with wheat straw arabinoxylan (AX) and β-glucan stearic acid ester (SABG) composite coating material in the concentration range of 1-2%.
32057861	1	38	dep	arabinoxylan	172:183	arg1	AX					186:187	AX	186:187	AX	186:187	Apples (Rich Red) were coated with wheat straw arabinoxylan (AX) and β-glucan stearic acid ester (SABG) composite coating material in the concentration range of 1-2%.
32057861	3	39	theme	respiration	523:533	arg1	loss					517:520	the weight loss	506:520	the weight loss	506:520	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	3	39	theme	respiration	523:533	arg1	process					563:569	process	563:569	process	563:569	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	3	39	theme	respiration	523:533	arg1	process					535:541	respiration process	523:541	respiration process	523:541	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	3	39	theme	respiration	523:533	arg1	degradation					550:560	color degradation	544:560	color degradation	544:560	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	2	40	theme	postharvest	296:306	arg1	life					316:319	The postharvest storage life	292:319	The postharvest storage life of coated apples	292:336	The postharvest storage life of coated apples was studied at 22 °C (±2) with 65% and 85% relative humidity for 45 days.
32057861	2	41	theme	relative	381:388	arg1	humidity					390:397	relative humidity	381:397	85% relative humidity for 45 days	377:409	The postharvest storage life of coated apples was studied at 22 °C (±2) with 65% and 85% relative humidity for 45 days.
32057861	1	42	theme	concentration	263:275	arg1	range					277:281	the concentration range	259:281	the concentration range of 1-2%	259:289	Apples (Rich Red) were coated with wheat straw arabinoxylan (AX) and β-glucan stearic acid ester (SABG) composite coating material in the concentration range of 1-2%.
32057861	3	43	theme	coatings	466:473	arg1	material					475:482	both AX-SABG (1-2%) and shellac (1-2%) coatings material	427:482	both AX-SABG (1-2%) and shellac (1-2%) coatings material	427:482	Application of both AX-SABG (1-2%) and shellac (1-2%) coatings material significantly reduced the weight loss, respiration process, color degradation, process of fruit softening and ripening index as compared to the uncoated apples up to 30 days storage.
32057861	4	44	theme	aroma	741:745	arg1	loss					747:750	the aroma loss	737:750	the aroma loss	737:750	However, the AX-SABG coatings were found more effective in preventing the aroma loss, reducing microbial spoilage and maintaining sensorial attributes as compared to shellac and uncoated apples during storage.
32337579	5	0	theme	labeled	727:733	arg1	monosaccharides					735:749	six 13C uniformly labeled monosaccharides	709:749	six 13C uniformly labeled monosaccharides	709:749	In this study, we traced the incorporation of six 13C uniformly labeled monosaccharides in the N-glycans, O-glycans and glycosphingolipids of both pluripotent and neural NTERA-2 cells.
32337579	8	1	theme	Co-feeding	1153:1162	arg1	studies					1164:1170	Co-feeding studies	1153:1170	Co-feeding studies	1153:1170	Co-feeding studies revealed that fructose incorporation is drastically decreased by the presence of glucose, while mannose and galactose were much less affected.
32337579	11	2	theme	isotope	1721:1727	arg1	labeling					1729:1736	metabolic isotope labeling	1711:1736	metabolic isotope labeling of glycans	1711:1747	We contend that the analysis of metabolic isotope labeling of glycans can yield new insights about cell metabolism.
32337579	1	3	theme	carbon	152:157	arg1	Saccharides					107:117	Saccharides	107:117	Saccharides in our diet	107:129	Saccharides in our diet are major sources of carbon for the formation of biomass such as proteins, lipids, nucleic acids and glycans.
32337579	1	3	theme	carbon	152:157	arg1	sources					141:147	major sources	135:147	major sources of carbon for the formation of biomass such as proteins, lipids, nucleic acids and glycans	135:238	Saccharides in our diet are major sources of carbon for the formation of biomass such as proteins, lipids, nucleic acids and glycans.
32337579	5	4	theme	monosaccharides	735:749	arg1	incorporation					692:704	the incorporation	688:704	the incorporation of six 13C uniformly labeled monosaccharides in the N-glycans, O-glycans and glycosphingolipids of both pluripotent and neural NTERA-2 cells	688:845	In this study, we traced the incorporation of six 13C uniformly labeled monosaccharides in the N-glycans, O-glycans and glycosphingolipids of both pluripotent and neural NTERA-2 cells.
32337579	8	5	theme	glucose	1253:1259	arg1	presence					1241:1248	the presence	1237:1248	the presence of glucose	1237:1259	Co-feeding studies revealed that fructose incorporation is drastically decreased by the presence of glucose, while mannose and galactose were much less affected.
32337579	10	6	theme	monosaccharide	1468:1481	arg1	utilization					1483:1493	exogenous monosaccharide utilization	1458:1493	exogenous monosaccharide utilization	1458:1493	Our results demonstrated that exogenous monosaccharide utilization can vary markedly depending on the cell differentiation state and monosaccharide availability, and that the incorporation of carbons can also differ among different glycan structures.
32337579	4	7	theme	supply	572:577	arg1	precursors					593:602	supply glycosylation precursors	572:602	supply glycosylation precursors	572:602	Applying metabolic flux analysis on glycan synthesis can reveal the pathways that supply glycosylation precursors and provide a snapshot of the metabolic state of the cell.
32337579	0	8	theme	monosaccharides	90:104	arg1	utilization					75:85	differential utilization	62:85	differential utilization of monosaccharides	62:104	Metabolic flux analysis of the neural cell glycocalyx reveals differential utilization of monosaccharides.
32337579	11	9	theme	labeling	1729:1736	arg1	analysis					1699:1706	the analysis	1695:1706	the analysis of metabolic isotope labeling of glycans	1695:1747	We contend that the analysis of metabolic isotope labeling of glycans can yield new insights about cell metabolism.
32337579	10	10	theme	exogenous	1458:1466	arg1	utilization					1483:1493	exogenous monosaccharide utilization	1458:1493	exogenous monosaccharide utilization	1458:1493	Our results demonstrated that exogenous monosaccharide utilization can vary markedly depending on the cell differentiation state and monosaccharide availability, and that the incorporation of carbons can also differ among different glycan structures.
32337579	2	11	theme	human	327:331	arg1	blood					333:337	human blood	327:337	human blood	327:337	Among the dietary monosaccharides, glucose occupies a central role in metabolism, but human blood contains regulated levels of other monosaccharides as well.
32337579	5	12	theme	NTERA-2	833:839	arg1	cells					841:845	both pluripotent and neural NTERA-2 cells	805:845	cells	841:845	In this study, we traced the incorporation of six 13C uniformly labeled monosaccharides in the N-glycans, O-glycans and glycosphingolipids of both pluripotent and neural NTERA-2 cells.
32337579	8	13	theme	fructose	1186:1193	arg1	incorporation					1195:1207	fructose incorporation	1186:1207	fructose incorporation	1186:1207	Co-feeding studies revealed that fructose incorporation is drastically decreased by the presence of glucose, while mannose and galactose were much less affected.
32337579	6	14	theme	isotopologue	869:880	arg1	data					882:885	detailed isotopologue data	860:885	detailed isotopologue data for hundreds of glycoconjugates	860:917	We gathered detailed isotopologue data for hundreds of glycoconjugates using mass spectrometry methods.
32337579	5	15	dep	N-glycans	758:766	arg1	the					754:756	the	754:756	the	754:756	In this study, we traced the incorporation of six 13C uniformly labeled monosaccharides in the N-glycans, O-glycans and glycosphingolipids of both pluripotent and neural NTERA-2 cells.
32337579	2	16	theme	dietary	251:257	arg1	monosaccharides					259:273	the dietary monosaccharides	247:273	the dietary monosaccharides	247:273	Among the dietary monosaccharides, glucose occupies a central role in metabolism, but human blood contains regulated levels of other monosaccharides as well.
32337579	2	17	theme	other	368:372	arg1	monosaccharides					374:388	other monosaccharides	368:388	other monosaccharides as well	368:396	Among the dietary monosaccharides, glucose occupies a central role in metabolism, but human blood contains regulated levels of other monosaccharides as well.
32337579	6	18	theme	detailed	860:867	arg1	data					882:885	detailed isotopologue data	860:885	detailed isotopologue data for hundreds of glycoconjugates	860:917	We gathered detailed isotopologue data for hundreds of glycoconjugates using mass spectrometry methods.
32337579	10	19	theme	different	1650:1658	arg1	structures					1667:1676	different glycan structures	1650:1676	different glycan structures	1650:1676	Our results demonstrated that exogenous monosaccharide utilization can vary markedly depending on the cell differentiation state and monosaccharide availability, and that the incorporation of carbons can also differ among different glycan structures.
32337579	4	20	theme	metabolic	634:642	arg1	state					644:648	the metabolic state	630:648	the metabolic state of the cell	630:660	Applying metabolic flux analysis on glycan synthesis can reveal the pathways that supply glycosylation precursors and provide a snapshot of the metabolic state of the cell.
32337579	0	21	theme	flux	10:13	arg1	analysis					15:22	Metabolic flux analysis	0:22	Metabolic flux analysis of the neural cell glycocalyx	0:52	Metabolic flux analysis of the neural cell glycocalyx reveals differential utilization of monosaccharides.
32337579	2	22	contain	contains	339:346	arg1	blood					333:337	human blood	327:337	human blood	327:337	Among the dietary monosaccharides, glucose occupies a central role in metabolism, but human blood contains regulated levels of other monosaccharides as well.
32337579	2	22	contain	contains	339:346	arg2	levels					358:363	regulated levels	348:363	regulated levels of other monosaccharides as well	348:396	Among the dietary monosaccharides, glucose occupies a central role in metabolism, but human blood contains regulated levels of other monosaccharides as well.
32337579	5	23	theme	13C	713:715	arg1	monosaccharides					735:749	six 13C uniformly labeled monosaccharides	709:749	six 13C uniformly labeled monosaccharides	709:749	In this study, we traced the incorporation of six 13C uniformly labeled monosaccharides in the N-glycans, O-glycans and glycosphingolipids of both pluripotent and neural NTERA-2 cells.
32337579	2	24	theme	central	295:301	arg1	role					303:306	a central role	293:306	a central role	293:306	Among the dietary monosaccharides, glucose occupies a central role in metabolism, but human blood contains regulated levels of other monosaccharides as well.
32337579	9	25	theme	increased	1328:1336	arg1	sialylation					1338:1348	increased sialylation	1328:1348	increased sialylation	1328:1348	Furthermore, increased sialylation slowed down the turnover of glycans, but fucosylation attenuated this effect.
32337579	0	26	theme	Metabolic	0:8	arg1	analysis					15:22	Metabolic flux analysis	0:22	Metabolic flux analysis of the neural cell glycocalyx	0:52	Metabolic flux analysis of the neural cell glycocalyx reveals differential utilization of monosaccharides.
32337579	7	27	dep	de	973:974	arg1	novo					976:979	novo	976:979	novo	976:979	The contributions of de novo synthesis and direct incorporation pathways for glucose, mannose, fructose, galactose, N-acetylglucosamine and fucose were determined based on their isotope incorporation.
32337579	2	28	theme	regulated	348:356	arg1	levels					358:363	regulated levels	348:363	regulated levels of other monosaccharides as well	348:396	Among the dietary monosaccharides, glucose occupies a central role in metabolism, but human blood contains regulated levels of other monosaccharides as well.
32337579	4	29	theme	glycan	526:531	arg1	synthesis					533:541	glycan synthesis	526:541	glycan synthesis	526:541	Applying metabolic flux analysis on glycan synthesis can reveal the pathways that supply glycosylation precursors and provide a snapshot of the metabolic state of the cell.
32337579	10	30	theme	differentiation	1535:1549	arg1	state					1551:1555	the cell differentiation state	1526:1555	the cell differentiation state	1526:1555	Our results demonstrated that exogenous monosaccharide utilization can vary markedly depending on the cell differentiation state and monosaccharide availability, and that the incorporation of carbons can also differ among different glycan structures.
32337579	5	31	theme	pluripotent	810:820	arg1	N-glycans					758:766	N-glycans	758:766	N-glycans	758:766	In this study, we traced the incorporation of six 13C uniformly labeled monosaccharides in the N-glycans, O-glycans and glycosphingolipids of both pluripotent and neural NTERA-2 cells.
32337579	5	31	theme	pluripotent	810:820	arg1	glycosphingolipids					783:800	glycosphingolipids	783:800	glycosphingolipids	783:800	In this study, we traced the incorporation of six 13C uniformly labeled monosaccharides in the N-glycans, O-glycans and glycosphingolipids of both pluripotent and neural NTERA-2 cells.
32337579	5	31	theme	pluripotent	810:820	arg1	O-glycans					769:777	O-glycans	769:777	O-glycans	769:777	In this study, we traced the incorporation of six 13C uniformly labeled monosaccharides in the N-glycans, O-glycans and glycosphingolipids of both pluripotent and neural NTERA-2 cells.
32337579	0	32	theme	neural	31:36	arg1	glycocalyx					43:52	the neural cell glycocalyx	27:52	the neural cell glycocalyx	27:52	Metabolic flux analysis of the neural cell glycocalyx reveals differential utilization of monosaccharides.
32337579	10	33	theme	cell	1530:1533	arg1	state					1551:1555	the cell differentiation state	1526:1555	the cell differentiation state	1526:1555	Our results demonstrated that exogenous monosaccharide utilization can vary markedly depending on the cell differentiation state and monosaccharide availability, and that the incorporation of carbons can also differ among different glycan structures.
32337579	11	34	theme	new	1759:1761	arg1	insights					1763:1770	new insights	1759:1770	new insights about cell metabolism	1759:1792	We contend that the analysis of metabolic isotope labeling of glycans can yield new insights about cell metabolism.
32337579	1	35	theme	biomass	180:186	arg1	formation					167:175	the formation	163:175	the formation of biomass such as proteins, lipids, nucleic acids and glycans	163:238	Saccharides in our diet are major sources of carbon for the formation of biomass such as proteins, lipids, nucleic acids and glycans.
32337579	4	36	theme	state	644:648	arg1	snapshot					618:625	a snapshot	616:625	a snapshot of the metabolic state of the cell	616:660	Applying metabolic flux analysis on glycan synthesis can reveal the pathways that supply glycosylation precursors and provide a snapshot of the metabolic state of the cell.
32337579	4	37	theme	flux	509:512	arg1	analysis					514:521	metabolic flux analysis	499:521	metabolic flux analysis	499:521	Applying metabolic flux analysis on glycan synthesis can reveal the pathways that supply glycosylation precursors and provide a snapshot of the metabolic state of the cell.
32337579	11	38	theme	glycans	1741:1747	arg1	labeling					1729:1736	metabolic isotope labeling	1711:1736	metabolic isotope labeling of glycans	1711:1747	We contend that the analysis of metabolic isotope labeling of glycans can yield new insights about cell metabolism.
32337579	3	39	used	utilized	446:453	arg2	they					437:440	they	437:440	they	437:440	Their influence on metabolism and how they are utilized have not been explored thoroughly.
32337579	7	40	theme	pathways	1016:1023	arg1	contributions					956:968	The contributions	952:968	The contributions of de novo synthesis and direct incorporation pathways for glucose, mannose, fructose, galactose, N-acetylglucosamine and fucose	952:1097	The contributions of de novo synthesis and direct incorporation pathways for glucose, mannose, fructose, galactose, N-acetylglucosamine and fucose were determined based on their isotope incorporation.
32337579	2	41	theme	monosaccharides	374:388	arg1	levels					358:363	regulated levels	348:363	regulated levels of other monosaccharides as well	348:396	Among the dietary monosaccharides, glucose occupies a central role in metabolism, but human blood contains regulated levels of other monosaccharides as well.
32337579	0	42	theme	glycocalyx	43:52	arg1	analysis					15:22	Metabolic flux analysis	0:22	Metabolic flux analysis of the neural cell glycocalyx	0:52	Metabolic flux analysis of the neural cell glycocalyx reveals differential utilization of monosaccharides.
32337579	4	43	theme	metabolic	499:507	arg1	analysis					514:521	metabolic flux analysis	499:521	metabolic flux analysis	499:521	Applying metabolic flux analysis on glycan synthesis can reveal the pathways that supply glycosylation precursors and provide a snapshot of the metabolic state of the cell.
32337579	5	44	from	incorporation	692:704	arg1	N-glycans					758:766	N-glycans	758:766	N-glycans	758:766	In this study, we traced the incorporation of six 13C uniformly labeled monosaccharides in the N-glycans, O-glycans and glycosphingolipids of both pluripotent and neural NTERA-2 cells.
32337579	5	44	from	incorporation	692:704	arg1	glycosphingolipids					783:800	glycosphingolipids	783:800	glycosphingolipids	783:800	In this study, we traced the incorporation of six 13C uniformly labeled monosaccharides in the N-glycans, O-glycans and glycosphingolipids of both pluripotent and neural NTERA-2 cells.
32337579	5	44	from	incorporation	692:704	arg1	O-glycans					769:777	O-glycans	769:777	O-glycans	769:777	In this study, we traced the incorporation of six 13C uniformly labeled monosaccharides in the N-glycans, O-glycans and glycosphingolipids of both pluripotent and neural NTERA-2 cells.
32337579	11	45	theme	metabolic	1711:1719	arg1	labeling					1729:1736	metabolic isotope labeling	1711:1736	metabolic isotope labeling of glycans	1711:1747	We contend that the analysis of metabolic isotope labeling of glycans can yield new insights about cell metabolism.
32337579	0	46	theme	cell	38:41	arg1	glycocalyx					43:52	the neural cell glycocalyx	27:52	the neural cell glycocalyx	27:52	Metabolic flux analysis of the neural cell glycocalyx reveals differential utilization of monosaccharides.
32337579	6	47	theme	mass	925:928	arg1	methods					943:949	mass spectrometry methods	925:949	mass spectrometry methods	925:949	We gathered detailed isotopologue data for hundreds of glycoconjugates using mass spectrometry methods.
32337579	11	48	theme	cell	1778:1781	arg1	metabolism					1783:1792	cell metabolism	1778:1792	cell metabolism	1778:1792	We contend that the analysis of metabolic isotope labeling of glycans can yield new insights about cell metabolism.
32337579	7	49	theme	direct	995:1000	arg1	pathways					1016:1023	direct incorporation pathways	995:1023	direct incorporation pathways for glucose, mannose, fructose, galactose, N-acetylglucosamine and fucose	995:1097	The contributions of de novo synthesis and direct incorporation pathways for glucose, mannose, fructose, galactose, N-acetylglucosamine and fucose were determined based on their isotope incorporation.
32337579	1	50	from	Saccharides	107:117	arg1	diet					126:129	our diet	122:129	our diet	122:129	Saccharides in our diet are major sources of carbon for the formation of biomass such as proteins, lipids, nucleic acids and glycans.
32337579	10	51	theme	monosaccharide	1561:1574	arg1	availability					1576:1587	monosaccharide availability	1561:1587	monosaccharide availability	1561:1587	Our results demonstrated that exogenous monosaccharide utilization can vary markedly depending on the cell differentiation state and monosaccharide availability, and that the incorporation of carbons can also differ among different glycan structures.
32337579	7	52	theme	incorporation	1002:1014	arg1	pathways					1016:1023	direct incorporation pathways	995:1023	direct incorporation pathways for glucose, mannose, fructose, galactose, N-acetylglucosamine and fucose	995:1097	The contributions of de novo synthesis and direct incorporation pathways for glucose, mannose, fructose, galactose, N-acetylglucosamine and fucose were determined based on their isotope incorporation.
32337579	7	53	theme	synthesis	981:989	arg1	contributions					956:968	The contributions	952:968	The contributions of de novo synthesis and direct incorporation pathways for glucose, mannose, fructose, galactose, N-acetylglucosamine and fucose	952:1097	The contributions of de novo synthesis and direct incorporation pathways for glucose, mannose, fructose, galactose, N-acetylglucosamine and fucose were determined based on their isotope incorporation.
32337579	1	54	theme	major	135:139	arg1	Saccharides					107:117	Saccharides	107:117	Saccharides in our diet	107:129	Saccharides in our diet are major sources of carbon for the formation of biomass such as proteins, lipids, nucleic acids and glycans.
32337579	1	54	theme	major	135:139	arg1	sources					141:147	major sources	135:147	major sources of carbon for the formation of biomass such as proteins, lipids, nucleic acids and glycans	135:238	Saccharides in our diet are major sources of carbon for the formation of biomass such as proteins, lipids, nucleic acids and glycans.
32337579	9	55	theme	glycans	1378:1384	arg1	turnover					1366:1373	the turnover	1362:1373	the turnover of glycans	1362:1384	Furthermore, increased sialylation slowed down the turnover of glycans, but fucosylation attenuated this effect.
32337579	4	56	theme	glycosylation	579:591	arg1	precursors					593:602	supply glycosylation precursors	572:602	supply glycosylation precursors	572:602	Applying metabolic flux analysis on glycan synthesis can reveal the pathways that supply glycosylation precursors and provide a snapshot of the metabolic state of the cell.
32337579	0	57	theme	differential	62:73	arg1	utilization					75:85	differential utilization	62:85	differential utilization of monosaccharides	62:104	Metabolic flux analysis of the neural cell glycocalyx reveals differential utilization of monosaccharides.
32337579	5	58	theme	neural	826:831	arg1	cells					841:845	both pluripotent and neural NTERA-2 cells	805:845	cells	841:845	In this study, we traced the incorporation of six 13C uniformly labeled monosaccharides in the N-glycans, O-glycans and glycosphingolipids of both pluripotent and neural NTERA-2 cells.
32337579	3	59	from	influence	405:413	arg1	utilized					446:453	utilized	446:453	are utilized	442:453	Their influence on metabolism and how they are utilized have not been explored thoroughly.
32337579	3	59	from	influence	405:413	arg1	metabolism					418:427	metabolism	418:427	metabolism	418:427	Their influence on metabolism and how they are utilized have not been explored thoroughly.
32337579	10	60	theme	carbons	1620:1626	arg1	incorporation					1603:1615	the incorporation	1599:1615	the incorporation of carbons	1599:1626	Our results demonstrated that exogenous monosaccharide utilization can vary markedly depending on the cell differentiation state and monosaccharide availability, and that the incorporation of carbons can also differ among different glycan structures.
32337579	5	61	theme	cells	841:845	arg1	N-glycans					758:766	N-glycans	758:766	N-glycans	758:766	In this study, we traced the incorporation of six 13C uniformly labeled monosaccharides in the N-glycans, O-glycans and glycosphingolipids of both pluripotent and neural NTERA-2 cells.
32337579	5	61	theme	cells	841:845	arg1	glycosphingolipids					783:800	glycosphingolipids	783:800	glycosphingolipids	783:800	In this study, we traced the incorporation of six 13C uniformly labeled monosaccharides in the N-glycans, O-glycans and glycosphingolipids of both pluripotent and neural NTERA-2 cells.
32337579	5	61	theme	cells	841:845	arg1	O-glycans					769:777	O-glycans	769:777	O-glycans	769:777	In this study, we traced the incorporation of six 13C uniformly labeled monosaccharides in the N-glycans, O-glycans and glycosphingolipids of both pluripotent and neural NTERA-2 cells.
32337579	1	62	theme	nucleic	214:220	arg1	acids					222:226	nucleic acids	214:226	nucleic acids	214:226	Saccharides in our diet are major sources of carbon for the formation of biomass such as proteins, lipids, nucleic acids and glycans.
32337579	7	63	theme	isotope	1130:1136	arg1	incorporation					1138:1150	their isotope incorporation	1124:1150	their isotope incorporation	1124:1150	The contributions of de novo synthesis and direct incorporation pathways for glucose, mannose, fructose, galactose, N-acetylglucosamine and fucose were determined based on their isotope incorporation.
32337579	6	64	theme	glycoconjugates	903:917	arg1	hundreds					891:898	hundreds	891:898	hundreds of glycoconjugates	891:917	We gathered detailed isotopologue data for hundreds of glycoconjugates using mass spectrometry methods.
32337579	10	65	theme	glycan	1660:1665	arg1	structures					1667:1676	different glycan structures	1650:1676	different glycan structures	1650:1676	Our results demonstrated that exogenous monosaccharide utilization can vary markedly depending on the cell differentiation state and monosaccharide availability, and that the incorporation of carbons can also differ among different glycan structures.
32337579	7	66	theme	de	973:974	arg1	synthesis					981:989	de novo synthesis	973:989	de novo synthesis	973:989	The contributions of de novo synthesis and direct incorporation pathways for glucose, mannose, fructose, galactose, N-acetylglucosamine and fucose were determined based on their isotope incorporation.
32337579	4	67	theme	cell	657:660	arg1	state					644:648	the metabolic state	630:648	the metabolic state of the cell	630:660	Applying metabolic flux analysis on glycan synthesis can reveal the pathways that supply glycosylation precursors and provide a snapshot of the metabolic state of the cell.
32337579	6	68	theme	spectrometry	930:941	arg1	methods					943:949	mass spectrometry methods	925:949	mass spectrometry methods	925:949	We gathered detailed isotopologue data for hundreds of glycoconjugates using mass spectrometry methods.
33463166	10	0	from	presence	1616:1623	arg1	solution					1640:1647	solution	1640:1647	solution	1640:1647	The presence of alginate in solution induced conformational changes in the cCBD-LL37 and LL37 peptides, resulting in increased peptide helicity, and reduced antimicrobial activity against P. aeruginosa.
33463166	7	1	theme	FIBRACOL	1124:1131	arg1	dressings					1113:1121	commercially available collagen-alginate wound dressings	1066:1121	commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing)	1066:1184	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	7	1	theme	FIBRACOL	1124:1131	arg1	Plus-a					1133:1138	FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing	1124:1183	FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing	1124:1183	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	2	2	theme	antimicrobial	354:366	arg1	resistance					368:377	antimicrobial resistance	354:377	antimicrobial resistance	354:377	Antibiotics and antiseptics are conventionally used to treat infected wounds; however, they can be toxic to mammalian cells, and their use can contribute to antimicrobial resistance.
33463166	6	3	theme	wound	806:810	arg1	composites					843:852	composites	843:852	composites with other materials, such as alginate	843:891	Collagen wound dressings are commonly made as composites with other materials, such as alginate.
33463166	6	3	theme	wound	806:810	arg1	dressings					812:820	Collagen wound dressings	797:820	Collagen wound dressings	797:820	Collagen wound dressings are commonly made as composites with other materials, such as alginate.
33463166	10	4	theme	LL37	1701:1704	arg1	peptides					1706:1713	the cCBD-LL37 and LL37 peptides	1683:1713	peptides	1706:1713	The presence of alginate in solution induced conformational changes in the cCBD-LL37 and LL37 peptides, resulting in increased peptide helicity, and reduced antimicrobial activity against P. aeruginosa.
33463166	12	5	theme	crucial	2043:2049	arg1	role					2051:2054	a crucial role	2041:2054	a crucial role	2041:2054	This study demonstrates that CBD-mediated LL37 tethering is a viable strategy to reduce LL37 toxicity, and how substrate composition plays a crucial role in modulating the antimicrobial activity of tethered AMPs.
33463166	12	6	theme	tethered	2100:2107	arg1	AMPs					2109:2112	tethered AMPs	2100:2112	tethered AMPs	2100:2112	This study demonstrates that CBD-mediated LL37 tethering is a viable strategy to reduce LL37 toxicity, and how substrate composition plays a crucial role in modulating the antimicrobial activity of tethered AMPs.
33463166	7	7	theme	collagen-alginate	1089:1105	arg1	dressings					1113:1121	commercially available collagen-alginate wound dressings	1066:1121	commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing)	1066:1184	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	7	7	theme	collagen-alginate	1089:1105	arg1	Plus-a					1133:1138	FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing	1124:1183	FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing	1124:1183	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	4	8	from	collagenase	572:582	arg1	domains					559:565	collagen-binding domains	542:565	collagen-binding domains from collagenase (cCBD) or fibronectin (fCBD)	542:611	In previous work, we modified the human AMP LL37 with collagen-binding domains from collagenase (cCBD) or fibronectin (fCBD) to facilitate peptide tethering and delivery from collagen-based wound dressings.
33463166	4	9	theme	wound	678:682	arg1	dressings					684:692	collagen-based wound dressings	663:692	collagen-based wound dressings	663:692	In previous work, we modified the human AMP LL37 with collagen-binding domains from collagenase (cCBD) or fibronectin (fCBD) to facilitate peptide tethering and delivery from collagen-based wound dressings.
33463166	9	10	theme	Plus	1368:1371	arg1	samples					1373:1379	FIBRACOL Plus samples	1359:1379	FIBRACOL Plus samples loaded with peptides	1359:1400	Additionally, FIBRACOL Plus samples loaded with peptides were bactericidal toward Pseudomonas aeruginosa, even after 14 days in release buffer but demonstrated no antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis.
33463166	4	11	theme	peptide	627:633	arg1	tethering					635:643	peptide tethering	627:643	peptide tethering	627:643	In previous work, we modified the human AMP LL37 with collagen-binding domains from collagenase (cCBD) or fibronectin (fCBD) to facilitate peptide tethering and delivery from collagen-based wound dressings.
33463166	10	12	theme	conformational	1657:1670	arg1	changes					1672:1678	conformational changes	1657:1678	conformational changes	1657:1678	The presence of alginate in solution induced conformational changes in the cCBD-LL37 and LL37 peptides, resulting in increased peptide helicity, and reduced antimicrobial activity against P. aeruginosa.
33463166	12	13	theme	CBD-mediated	1931:1942	arg1	tethering					1949:1957	CBD-mediated LL37 tethering	1931:1957	CBD-mediated LL37 tethering	1931:1957	This study demonstrates that CBD-mediated LL37 tethering is a viable strategy to reduce LL37 toxicity, and how substrate composition plays a crucial role in modulating the antimicrobial activity of tethered AMPs.
33463166	12	13	theme	CBD-mediated	1931:1942	arg1	strategy					1971:1978	a viable strategy	1962:1978	a viable strategy to reduce LL37 toxicity	1962:2002	This study demonstrates that CBD-mediated LL37 tethering is a viable strategy to reduce LL37 toxicity, and how substrate composition plays a crucial role in modulating the antimicrobial activity of tethered AMPs.
33463166	8	14	theme	fCBD-LL37	1238:1246	arg1	cCBD-LL37					1223:1231	cCBD-LL37	1223:1231	cCBD-LL37	1223:1231	We found that over 85% of the LL37, cCBD-LL37, and fCBD-LL37 was retained on FIBRACOL Plus over a 14-day release study (90.3, 85.8, and 98.6%, respectively).
33463166	8	14	theme	fCBD-LL37	1238:1246	arg1	%					1208:1208	over 85%	1201:1208	over 85% of the LL37, cCBD-LL37, and fCBD-LL37	1201:1246	We found that over 85% of the LL37, cCBD-LL37, and fCBD-LL37 was retained on FIBRACOL Plus over a 14-day release study (90.3, 85.8, and 98.6%, respectively).
33463166	8	14	theme	fCBD-LL37	1238:1246	arg1	LL37					1217:1220	LL37	1217:1220	LL37	1217:1220	We found that over 85% of the LL37, cCBD-LL37, and fCBD-LL37 was retained on FIBRACOL Plus over a 14-day release study (90.3, 85.8, and 98.6%, respectively).
33463166	8	14	theme	fCBD-LL37	1238:1246	arg1	fCBD-LL37					1238:1246	fCBD-LL37	1238:1246	fCBD-LL37	1238:1246	We found that over 85% of the LL37, cCBD-LL37, and fCBD-LL37 was retained on FIBRACOL Plus over a 14-day release study (90.3, 85.8, and 98.6%, respectively).
33463166	10	15	theme	peptide	1739:1745	arg1	helicity					1747:1754	increased peptide helicity	1729:1754	increased peptide helicity	1729:1754	The presence of alginate in solution induced conformational changes in the cCBD-LL37 and LL37 peptides, resulting in increased peptide helicity, and reduced antimicrobial activity against P. aeruginosa.
33463166	12	16	theme	substrate	2013:2021	arg1	composition					2023:2033	substrate composition	2013:2033	substrate composition	2013:2033	This study demonstrates that CBD-mediated LL37 tethering is a viable strategy to reduce LL37 toxicity, and how substrate composition plays a crucial role in modulating the antimicrobial activity of tethered AMPs.
33463166	0	17	theme	Peptides	77:84	arg1	Bioactivity					17:27	Bioactivity	17:27	Bioactivity of Chimeric Collagen-Binding LL37 Antimicrobial Peptides Adsorbed to Collagen-Alginate Wound Dressings	17:130	Alginate Affects Bioactivity of Chimeric Collagen-Binding LL37 Antimicrobial Peptides Adsorbed to Collagen-Alginate Wound Dressings.
33463166	7	18	theme	alginate	956:963	arg1	presence					944:951	the presence	940:951	the presence of alginate	940:963	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	2	19	used	used	244:247	arg2	antiseptics					213:223	antiseptics	213:223	antiseptics	213:223	Antibiotics and antiseptics are conventionally used to treat infected wounds; however, they can be toxic to mammalian cells, and their use can contribute to antimicrobial resistance.
33463166	2	19	used	used	244:247	arg2	Antibiotics					197:207	Antibiotics	197:207	Antibiotics	197:207	Antibiotics and antiseptics are conventionally used to treat infected wounds; however, they can be toxic to mammalian cells, and their use can contribute to antimicrobial resistance.
33463166	10	20	attach	presence	1616:1623	arg1	solution					1640:1647	solution	1640:1647	solution	1640:1647	The presence of alginate in solution induced conformational changes in the cCBD-LL37 and LL37 peptides, resulting in increased peptide helicity, and reduced antimicrobial activity against P. aeruginosa.
33463166	10	20	attach	presence	1616:1623	arg2	alginate					1628:1635	alginate	1628:1635	alginate	1628:1635	The presence of alginate in solution induced conformational changes in the cCBD-LL37 and LL37 peptides, resulting in increased peptide helicity, and reduced antimicrobial activity against P. aeruginosa.
33463166	5	21	theme	100	772:774	arg1	%					775:775	%	775:775	%	775:775	We found that cCBD-LL37 and fCBD-LL37 were retained and active when bound to 100% collagen scaffolds.
33463166	8	22	theme	14-day	1285:1290	arg1	study					1300:1304	a 14-day release study	1283:1304	a 14-day release study (90.3, 85.8, and 98.6%, respectively)	1283:1342	We found that over 85% of the LL37, cCBD-LL37, and fCBD-LL37 was retained on FIBRACOL Plus over a 14-day release study (90.3, 85.8, and 98.6%, respectively).
33463166	0	23	theme	Wound	116:120	arg1	Dressings					122:130	Collagen-Alginate Wound Dressings	98:130	Collagen-Alginate Wound Dressings	98:130	Alginate Affects Bioactivity of Chimeric Collagen-Binding LL37 Antimicrobial Peptides Adsorbed to Collagen-Alginate Wound Dressings.
33463166	7	24	dep	Plus-a	1133:1138	arg1	collagen					1144:1151	90% collagen	1140:1151	90% collagen	1140:1151	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	7	24	dep	Plus-a	1133:1138	arg1	dressing					1176:1183	10% alginate wound dressing	1157:1183	10% alginate wound dressing	1157:1183	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	7	25	theme	%	1142:1142	arg1	collagen					1144:1151	90% collagen	1140:1151	90% collagen	1140:1151	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	9	26	theme	release	1473:1479	arg1	buffer					1481:1486	release buffer	1473:1486	release buffer	1473:1486	Additionally, FIBRACOL Plus samples loaded with peptides were bactericidal toward Pseudomonas aeruginosa, even after 14 days in release buffer but demonstrated no antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis.
33463166	7	27	theme	alginate	1161:1168	arg1	dressing					1176:1183	10% alginate wound dressing	1157:1183	10% alginate wound dressing	1157:1183	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	7	28	theme	study	911:915	arg1	goal					898:901	The goal	894:901	The goal of this study	894:915	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	3	29	theme	antibiotics	475:485	arg1	limitations					444:454	the limitations	440:454	the limitations of antiseptics and antibiotics	440:485	Antimicrobial peptides (AMPs) have been utilized to address the limitations of antiseptics and antibiotics.
33463166	7	30	theme	10	1157:1158	arg1	%					1159:1159	%	1159:1159	%	1159:1159	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	1	31	theme	Chronic	133:139	arg1	wounds					150:155	Chronic infected wounds	133:155	Chronic infected wounds	133:155	Chronic infected wounds cause more than 23,000 deaths annually.
33463166	8	32	theme	FIBRACOL	1264:1271	arg1	Plus					1273:1276	FIBRACOL Plus	1264:1276	FIBRACOL Plus	1264:1276	We found that over 85% of the LL37, cCBD-LL37, and fCBD-LL37 was retained on FIBRACOL Plus over a 14-day release study (90.3, 85.8, and 98.6%, respectively).
33463166	12	33	dep	strategy	1971:1978	arg1	plays					2035:2039	plays	2035:2039	plays a crucial role in modulating the antimicrobial activity of tethered AMPs	2035:2112	This study demonstrates that CBD-mediated LL37 tethering is a viable strategy to reduce LL37 toxicity, and how substrate composition plays a crucial role in modulating the antimicrobial activity of tethered AMPs.
33463166	3	34	theme	antiseptics	459:469	arg1	limitations					444:454	the limitations	440:454	the limitations of antiseptics and antibiotics	440:485	Antimicrobial peptides (AMPs) have been utilized to address the limitations of antiseptics and antibiotics.
33463166	7	35	dep	tethering	977:985	arg1	the					973:975	the	973:975	the	973:975	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	11	36	theme	dermal	1882:1887	arg1	fibroblasts					1889:1899	human dermal fibroblasts	1876:1899	human dermal fibroblasts	1876:1899	Peptide-loaded FIBRACOL Plus scaffolds were not cytotoxic to human dermal fibroblasts.
33463166	0	37	theme	Chimeric	32:39	arg1	Peptides					77:84	Chimeric Collagen-Binding LL37 Antimicrobial Peptides	32:84	Chimeric Collagen-Binding LL37 Antimicrobial Peptides Adsorbed to Collagen-Alginate Wound Dressings	32:130	Alginate Affects Bioactivity of Chimeric Collagen-Binding LL37 Antimicrobial Peptides Adsorbed to Collagen-Alginate Wound Dressings.
33463166	5	38	theme	collagen	777:784	arg1	scaffolds					786:794	100% collagen scaffolds	772:794	100% collagen scaffolds	772:794	We found that cCBD-LL37 and fCBD-LL37 were retained and active when bound to 100% collagen scaffolds.
33463166	6	39	theme	other	859:863	arg1	alginate					884:891	alginate	884:891	alginate	884:891	Collagen wound dressings are commonly made as composites with other materials, such as alginate.
33463166	6	39	theme	other	859:863	arg1	materials					865:873	other materials	859:873	other materials	859:873	Collagen wound dressings are commonly made as composites with other materials, such as alginate.
33463166	4	40	theme	AMP	528:530	arg1	LL37					532:535	the human AMP LL37	518:535	the human AMP LL37 with collagen-binding domains from collagenase (cCBD) or fibronectin (fCBD) to facilitate peptide tethering and delivery from collagen-based wound dressings	518:692	In previous work, we modified the human AMP LL37 with collagen-binding domains from collagenase (cCBD) or fibronectin (fCBD) to facilitate peptide tethering and delivery from collagen-based wound dressings.
33463166	7	41	theme	90	1140:1141	arg1	%					1142:1142	%	1142:1142	%	1142:1142	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	10	42	theme	cCBD-LL37	1687:1695	arg1	peptides					1706:1713	the cCBD-LL37 and LL37 peptides	1683:1713	peptides	1706:1713	The presence of alginate in solution induced conformational changes in the cCBD-LL37 and LL37 peptides, resulting in increased peptide helicity, and reduced antimicrobial activity against P. aeruginosa.
33463166	12	43	theme	LL37	1990:1993	arg1	toxicity					1995:2002	LL37 toxicity	1990:2002	LL37 toxicity	1990:2002	This study demonstrates that CBD-mediated LL37 tethering is a viable strategy to reduce LL37 toxicity, and how substrate composition plays a crucial role in modulating the antimicrobial activity of tethered AMPs.
33463166	11	44	theme	FIBRACOL	1830:1837	arg1	scaffolds					1844:1852	Peptide-loaded FIBRACOL Plus scaffolds	1815:1852	Peptide-loaded FIBRACOL Plus scaffolds	1815:1852	Peptide-loaded FIBRACOL Plus scaffolds were not cytotoxic to human dermal fibroblasts.
33463166	7	45	theme	wound	1107:1111	arg1	dressings					1113:1121	commercially available collagen-alginate wound dressings	1066:1121	commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing)	1066:1184	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	7	45	theme	wound	1107:1111	arg1	Plus-a					1133:1138	FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing	1124:1183	FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing	1124:1183	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	2	46	theme	mammalian	305:313	arg1	cells					315:319	mammalian cells	305:319	mammalian cells	305:319	Antibiotics and antiseptics are conventionally used to treat infected wounds; however, they can be toxic to mammalian cells, and their use can contribute to antimicrobial resistance.
33463166	12	47	theme	viable	1964:1969	arg1	tethering					1949:1957	CBD-mediated LL37 tethering	1931:1957	CBD-mediated LL37 tethering	1931:1957	This study demonstrates that CBD-mediated LL37 tethering is a viable strategy to reduce LL37 toxicity, and how substrate composition plays a crucial role in modulating the antimicrobial activity of tethered AMPs.
33463166	12	47	theme	viable	1964:1969	arg1	strategy					1971:1978	a viable strategy	1962:1978	a viable strategy to reduce LL37 toxicity	1962:2002	This study demonstrates that CBD-mediated LL37 tethering is a viable strategy to reduce LL37 toxicity, and how substrate composition plays a crucial role in modulating the antimicrobial activity of tethered AMPs.
33463166	7	48	theme	available	1079:1087	arg1	dressings					1113:1121	commercially available collagen-alginate wound dressings	1066:1121	commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing)	1066:1184	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	7	48	theme	available	1079:1087	arg1	Plus-a					1133:1138	FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing	1124:1183	FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing	1124:1183	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	6	49	theme	Collagen	797:804	arg1	composites					843:852	composites	843:852	composites with other materials, such as alginate	843:891	Collagen wound dressings are commonly made as composites with other materials, such as alginate.
33463166	6	49	theme	Collagen	797:804	arg1	dressings					812:820	Collagen wound dressings	797:820	Collagen wound dressings	797:820	Collagen wound dressings are commonly made as composites with other materials, such as alginate.
33463166	0	50	theme	LL37	58:61	arg1	Peptides					77:84	Chimeric Collagen-Binding LL37 Antimicrobial Peptides	32:84	Chimeric Collagen-Binding LL37 Antimicrobial Peptides Adsorbed to Collagen-Alginate Wound Dressings	32:130	Alginate Affects Bioactivity of Chimeric Collagen-Binding LL37 Antimicrobial Peptides Adsorbed to Collagen-Alginate Wound Dressings.
33463166	10	51	from	changes	1672:1678	arg1	peptides					1706:1713	the cCBD-LL37 and LL37 peptides	1683:1713	peptides	1706:1713	The presence of alginate in solution induced conformational changes in the cCBD-LL37 and LL37 peptides, resulting in increased peptide helicity, and reduced antimicrobial activity against P. aeruginosa.
33463166	10	52	theme	increased	1729:1737	arg1	helicity					1747:1754	increased peptide helicity	1729:1754	increased peptide helicity	1729:1754	The presence of alginate in solution induced conformational changes in the cCBD-LL37 and LL37 peptides, resulting in increased peptide helicity, and reduced antimicrobial activity against P. aeruginosa.
33463166	9	53	theme	FIBRACOL	1359:1366	arg1	samples					1373:1379	FIBRACOL Plus samples	1359:1379	FIBRACOL Plus samples loaded with peptides	1359:1400	Additionally, FIBRACOL Plus samples loaded with peptides were bactericidal toward Pseudomonas aeruginosa, even after 14 days in release buffer but demonstrated no antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis.
33463166	12	54	theme	LL37	1944:1947	arg1	tethering					1949:1957	CBD-mediated LL37 tethering	1931:1957	CBD-mediated LL37 tethering	1931:1957	This study demonstrates that CBD-mediated LL37 tethering is a viable strategy to reduce LL37 toxicity, and how substrate composition plays a crucial role in modulating the antimicrobial activity of tethered AMPs.
33463166	12	54	theme	LL37	1944:1947	arg1	strategy					1971:1978	a viable strategy	1962:1978	a viable strategy to reduce LL37 toxicity	1962:2002	This study demonstrates that CBD-mediated LL37 tethering is a viable strategy to reduce LL37 toxicity, and how substrate composition plays a crucial role in modulating the antimicrobial activity of tethered AMPs.
33463166	12	55	theme	AMPs	2109:2112	arg1	activity					2088:2095	the antimicrobial activity	2070:2095	the antimicrobial activity of tethered AMPs	2070:2112	This study demonstrates that CBD-mediated LL37 tethering is a viable strategy to reduce LL37 toxicity, and how substrate composition plays a crucial role in modulating the antimicrobial activity of tethered AMPs.
33463166	7	56	theme	peptides	1045:1052	arg1	release					988:994	release	988:994	release	988:994	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	7	56	theme	peptides	1045:1052	arg1	tethering					977:985	tethering	977:985	tethering	977:985	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	7	56	theme	peptides	1045:1052	arg1	activity					1015:1022	antimicrobial activity	1001:1022	antimicrobial activity	1001:1022	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	4	57	with	LL37	532:535	arg1	domains					559:565	collagen-binding domains	542:565	collagen-binding domains from collagenase (cCBD) or fibronectin (fCBD)	542:611	In previous work, we modified the human AMP LL37 with collagen-binding domains from collagenase (cCBD) or fibronectin (fCBD) to facilitate peptide tethering and delivery from collagen-based wound dressings.
33463166	8	58	dep	study	1300:1304	arg1	%					1327:1327	90.3, 85.8, and 98.6%	1307:1327	90.3, 85.8, and 98.6%	1307:1327	We found that over 85% of the LL37, cCBD-LL37, and fCBD-LL37 was retained on FIBRACOL Plus over a 14-day release study (90.3, 85.8, and 98.6%, respectively).
33463166	8	59	theme	LL37	1217:1220	arg1	cCBD-LL37					1223:1231	cCBD-LL37	1223:1231	cCBD-LL37	1223:1231	We found that over 85% of the LL37, cCBD-LL37, and fCBD-LL37 was retained on FIBRACOL Plus over a 14-day release study (90.3, 85.8, and 98.6%, respectively).
33463166	8	59	theme	LL37	1217:1220	arg1	%					1208:1208	over 85%	1201:1208	over 85% of the LL37, cCBD-LL37, and fCBD-LL37	1201:1246	We found that over 85% of the LL37, cCBD-LL37, and fCBD-LL37 was retained on FIBRACOL Plus over a 14-day release study (90.3, 85.8, and 98.6%, respectively).
33463166	8	59	theme	LL37	1217:1220	arg1	LL37					1217:1220	LL37	1217:1220	LL37	1217:1220	We found that over 85% of the LL37, cCBD-LL37, and fCBD-LL37 was retained on FIBRACOL Plus over a 14-day release study (90.3, 85.8, and 98.6%, respectively).
33463166	8	59	theme	LL37	1217:1220	arg1	fCBD-LL37					1238:1246	fCBD-LL37	1238:1246	fCBD-LL37	1238:1246	We found that over 85% of the LL37, cCBD-LL37, and fCBD-LL37 was retained on FIBRACOL Plus over a 14-day release study (90.3, 85.8, and 98.6%, respectively).
33463166	4	60	theme	collagen-based	663:676	arg1	dressings					684:692	collagen-based wound dressings	663:692	collagen-based wound dressings	663:692	In previous work, we modified the human AMP LL37 with collagen-binding domains from collagenase (cCBD) or fibronectin (fCBD) to facilitate peptide tethering and delivery from collagen-based wound dressings.
33463166	8	61	theme	cCBD-LL37	1223:1231	arg1	cCBD-LL37					1223:1231	cCBD-LL37	1223:1231	cCBD-LL37	1223:1231	We found that over 85% of the LL37, cCBD-LL37, and fCBD-LL37 was retained on FIBRACOL Plus over a 14-day release study (90.3, 85.8, and 98.6%, respectively).
33463166	8	61	theme	cCBD-LL37	1223:1231	arg1	%					1208:1208	over 85%	1201:1208	over 85% of the LL37, cCBD-LL37, and fCBD-LL37	1201:1246	We found that over 85% of the LL37, cCBD-LL37, and fCBD-LL37 was retained on FIBRACOL Plus over a 14-day release study (90.3, 85.8, and 98.6%, respectively).
33463166	8	61	theme	cCBD-LL37	1223:1231	arg1	LL37					1217:1220	LL37	1217:1220	LL37	1217:1220	We found that over 85% of the LL37, cCBD-LL37, and fCBD-LL37 was retained on FIBRACOL Plus over a 14-day release study (90.3, 85.8, and 98.6%, respectively).
33463166	8	61	theme	cCBD-LL37	1223:1231	arg1	fCBD-LL37					1238:1246	fCBD-LL37	1238:1246	fCBD-LL37	1238:1246	We found that over 85% of the LL37, cCBD-LL37, and fCBD-LL37 was retained on FIBRACOL Plus over a 14-day release study (90.3, 85.8, and 98.6%, respectively).
33463166	2	62	theme	infected	258:265	arg1	wounds					267:272	infected wounds	258:272	infected wounds	258:272	Antibiotics and antiseptics are conventionally used to treat infected wounds; however, they can be toxic to mammalian cells, and their use can contribute to antimicrobial resistance.
33463166	1	63	theme	infected	141:148	arg1	wounds					150:155	Chronic infected wounds	133:155	Chronic infected wounds	133:155	Chronic infected wounds cause more than 23,000 deaths annually.
33463166	7	64	theme	antimicrobial	1001:1013	arg1	activity					1015:1022	antimicrobial activity	1001:1022	antimicrobial activity	1001:1022	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	0	65	theme	Collagen-Binding	41:56	arg1	Peptides					77:84	Chimeric Collagen-Binding LL37 Antimicrobial Peptides	32:84	Chimeric Collagen-Binding LL37 Antimicrobial Peptides Adsorbed to Collagen-Alginate Wound Dressings	32:130	Alginate Affects Bioactivity of Chimeric Collagen-Binding LL37 Antimicrobial Peptides Adsorbed to Collagen-Alginate Wound Dressings.
33463166	10	66	theme	antimicrobial	1769:1781	arg1	activity					1783:1790	antimicrobial activity	1769:1790	antimicrobial activity against P. aeruginosa	1769:1812	The presence of alginate in solution induced conformational changes in the cCBD-LL37 and LL37 peptides, resulting in increased peptide helicity, and reduced antimicrobial activity against P. aeruginosa.
33463166	0	67	theme	Collagen-Alginate	98:114	arg1	Dressings					122:130	Collagen-Alginate Wound Dressings	98:130	Collagen-Alginate Wound Dressings	98:130	Alginate Affects Bioactivity of Chimeric Collagen-Binding LL37 Antimicrobial Peptides Adsorbed to Collagen-Alginate Wound Dressings.
33463166	11	68	theme	Plus	1839:1842	arg1	scaffolds					1844:1852	Peptide-loaded FIBRACOL Plus scaffolds	1815:1852	Peptide-loaded FIBRACOL Plus scaffolds	1815:1852	Peptide-loaded FIBRACOL Plus scaffolds were not cytotoxic to human dermal fibroblasts.
33463166	3	69	used	utilized	420:427	arg2	peptides					394:401	Antimicrobial peptides	380:401	Antimicrobial peptides (AMPs)	380:408	Antimicrobial peptides (AMPs) have been utilized to address the limitations of antiseptics and antibiotics.
33463166	3	69	used	utilized	420:427	arg2	AMPs					404:407	AMPs	404:407	AMPs	404:407	Antimicrobial peptides (AMPs) have been utilized to address the limitations of antiseptics and antibiotics.
33463166	1	70	theme	deaths	180:185	arg1	annually					187:194	more than 23,000 deaths annually	163:194	more than 23,000 deaths annually	163:194	Chronic infected wounds cause more than 23,000 deaths annually.
33463166	4	71	theme	human	522:526	arg1	LL37					532:535	the human AMP LL37	518:535	the human AMP LL37 with collagen-binding domains from collagenase (cCBD) or fibronectin (fCBD) to facilitate peptide tethering and delivery from collagen-based wound dressings	518:692	In previous work, we modified the human AMP LL37 with collagen-binding domains from collagenase (cCBD) or fibronectin (fCBD) to facilitate peptide tethering and delivery from collagen-based wound dressings.
33463166	4	72	from	fibronectin	594:604	arg1	domains					559:565	collagen-binding domains	542:565	collagen-binding domains from collagenase (cCBD) or fibronectin (fCBD)	542:611	In previous work, we modified the human AMP LL37 with collagen-binding domains from collagenase (cCBD) or fibronectin (fCBD) to facilitate peptide tethering and delivery from collagen-based wound dressings.
33463166	9	73	from	days	1465:1468	arg1	buffer					1481:1486	release buffer	1473:1486	release buffer	1473:1486	Additionally, FIBRACOL Plus samples loaded with peptides were bactericidal toward Pseudomonas aeruginosa, even after 14 days in release buffer but demonstrated no antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis.
33463166	3	74	theme	Antimicrobial	380:392	arg1	AMPs					404:407	AMPs	404:407	AMPs	404:407	Antimicrobial peptides (AMPs) have been utilized to address the limitations of antiseptics and antibiotics.
33463166	3	74	theme	Antimicrobial	380:392	arg1	peptides					394:401	Antimicrobial peptides	380:401	Antimicrobial peptides (AMPs)	380:408	Antimicrobial peptides (AMPs) have been utilized to address the limitations of antiseptics and antibiotics.
33463166	4	75	theme	previous	491:498	arg1	work					500:503	previous work	491:503	previous work	491:503	In previous work, we modified the human AMP LL37 with collagen-binding domains from collagenase (cCBD) or fibronectin (fCBD) to facilitate peptide tethering and delivery from collagen-based wound dressings.
33463166	7	76	theme	wound	1170:1174	arg1	dressing					1176:1183	10% alginate wound dressing	1157:1183	10% alginate wound dressing	1157:1183	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	8	77	theme	release	1292:1298	arg1	study					1300:1304	a 14-day release study	1283:1304	a 14-day release study (90.3, 85.8, and 98.6%, respectively)	1283:1342	We found that over 85% of the LL37, cCBD-LL37, and fCBD-LL37 was retained on FIBRACOL Plus over a 14-day release study (90.3, 85.8, and 98.6%, respectively).
33463166	11	78	theme	human	1876:1880	arg1	fibroblasts					1889:1899	human dermal fibroblasts	1876:1899	human dermal fibroblasts	1876:1899	Peptide-loaded FIBRACOL Plus scaffolds were not cytotoxic to human dermal fibroblasts.
33463166	7	79	theme	%	1159:1159	arg1	dressing					1176:1183	10% alginate wound dressing	1157:1183	10% alginate wound dressing	1157:1183	The goal of this study was to investigate how the presence of alginate affects the tethering, release, and antimicrobial activity of LL37 and CBD-LL37 peptides adsorbed to commercially available collagen-alginate wound dressings (FIBRACOL Plus-a 90% collagen and 10% alginate wound dressing).
33463166	5	80	theme	%	775:775	arg1	scaffolds					786:794	100% collagen scaffolds	772:794	100% collagen scaffolds	772:794	We found that cCBD-LL37 and fCBD-LL37 were retained and active when bound to 100% collagen scaffolds.
33463166	9	81	theme	antimicrobial	1508:1520	arg1	activity					1522:1529	no antimicrobial activity	1505:1529	no antimicrobial activity	1505:1529	Additionally, FIBRACOL Plus samples loaded with peptides were bactericidal toward Pseudomonas aeruginosa, even after 14 days in release buffer but demonstrated no antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis.
33463166	6	82	with	composites	843:852	arg1	alginate					884:891	alginate	884:891	alginate	884:891	Collagen wound dressings are commonly made as composites with other materials, such as alginate.
33463166	6	82	with	composites	843:852	arg1	materials					865:873	other materials	859:873	other materials	859:873	Collagen wound dressings are commonly made as composites with other materials, such as alginate.
33463166	0	83	theme	Antimicrobial	63:75	arg1	Peptides					77:84	Chimeric Collagen-Binding LL37 Antimicrobial Peptides	32:84	Chimeric Collagen-Binding LL37 Antimicrobial Peptides Adsorbed to Collagen-Alginate Wound Dressings	32:130	Alginate Affects Bioactivity of Chimeric Collagen-Binding LL37 Antimicrobial Peptides Adsorbed to Collagen-Alginate Wound Dressings.
33463166	4	84	theme	collagen-binding	542:557	arg1	domains					559:565	collagen-binding domains	542:565	collagen-binding domains from collagenase (cCBD) or fibronectin (fCBD)	542:611	In previous work, we modified the human AMP LL37 with collagen-binding domains from collagenase (cCBD) or fibronectin (fCBD) to facilitate peptide tethering and delivery from collagen-based wound dressings.
33463166	11	85	theme	Peptide-loaded	1815:1828	arg1	scaffolds					1844:1852	Peptide-loaded FIBRACOL Plus scaffolds	1815:1852	Peptide-loaded FIBRACOL Plus scaffolds	1815:1852	Peptide-loaded FIBRACOL Plus scaffolds were not cytotoxic to human dermal fibroblasts.
33463166	8	86	dep	LL37	1217:1220	arg1	the					1213:1215	the	1213:1215	the	1213:1215	We found that over 85% of the LL37, cCBD-LL37, and fCBD-LL37 was retained on FIBRACOL Plus over a 14-day release study (90.3, 85.8, and 98.6%, respectively).
33463166	10	87	theme	alginate	1628:1635	arg1	presence					1616:1623	The presence	1612:1623	The presence of alginate in solution	1612:1647	The presence of alginate in solution induced conformational changes in the cCBD-LL37 and LL37 peptides, resulting in increased peptide helicity, and reduced antimicrobial activity against P. aeruginosa.
33463166	12	88	theme	antimicrobial	2074:2086	arg1	activity					2088:2095	the antimicrobial activity	2070:2095	the antimicrobial activity of tethered AMPs	2070:2112	This study demonstrates that CBD-mediated LL37 tethering is a viable strategy to reduce LL37 toxicity, and how substrate composition plays a crucial role in modulating the antimicrobial activity of tethered AMPs.
33345720	7	0	theme	ALG	1356:1358	arg1	CMC-3					1360:1364	@CMC-2 and ALG@CMC-3	1345:1364	CMC-3	1360:1364	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	6	1	theme	ALG	1188:1190	arg1	beads					1196:1200	ALG@CMC beads	1188:1200	ALG@CMC beads	1188:1200	The swelling and drug release behaviors revealed that the swelling and drug release rate of ALG@CMC beads were obviously slower than that of simple-ALG and both have significant pH responsiveness.
33345720	1	2	theme	cellulose	261:269	arg1	CMC					288:290	ALG@CMC	284:290	ALG@CMC	284:290	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	1	2	theme	cellulose	261:269	arg1	shell					277:281	carboxymethyl cellulose (CMC) shell	247:281	carboxymethyl cellulose (CMC) shell (ALG@CMC)	247:291	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	1	3	theme	CMC	272:274	arg1	CMC					288:290	ALG@CMC	284:290	ALG@CMC	284:290	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	1	3	theme	CMC	272:274	arg1	shell					277:281	carboxymethyl cellulose (CMC) shell	247:281	carboxymethyl cellulose (CMC) shell (ALG@CMC)	247:291	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	4	4	dep	buffered	697:704	arg1	phosphate					687:695	phosphate	687:695	phosphate	687:695	The mechanical stability of all samples in phosphate buffered solution (PBS, pH 7.4) was approximately measured through oscillation experiments.
33345720	6	5	theme	drug	1167:1170	arg1	release					1172:1178	drug release	1167:1178	drug release	1167:1178	The swelling and drug release behaviors revealed that the swelling and drug release rate of ALG@CMC beads were obviously slower than that of simple-ALG and both have significant pH responsiveness.
33345720	5	6	theme	drug	813:816	arg1	release					818:824	controlled drug release	802:824	controlled drug release	802:824	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	7	7	theme	drug	1308:1311	arg1	%					1379:1379	about 100%	1370:1379	about 100%	1370:1379	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	7	7	theme	drug	1308:1311	arg1	release					1313:1319	The cumulative drug release	1293:1319	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3	1293:1364	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	6	8	theme	swelling	1154:1161	arg1	slower					1217:1222	slower	1217:1222	slower	1217:1222	The swelling and drug release behaviors revealed that the swelling and drug release rate of ALG@CMC beads were obviously slower than that of simple-ALG and both have significant pH responsiveness.
33345720	6	8	theme	swelling	1154:1161	arg1	rate					1180:1183	the swelling and drug release rate	1150:1183	the swelling and drug release rate of ALG@CMC beads	1150:1200	The swelling and drug release behaviors revealed that the swelling and drug release rate of ALG@CMC beads were obviously slower than that of simple-ALG and both have significant pH responsiveness.
33345720	3	9	theme	infrared	540:547	arg1	spectroscopy					556:567	infrared (FTIR) spectroscopy	540:567	infrared (FTIR) spectroscopy	540:567	The resulting gel samples were characterized by Fourier transforms infrared (FTIR) spectroscopy, thermo-gravimetric (TG) analysis, and scanning electron microscopy (SEM).
33345720	6	10	contain	have	1257:1260	arg1	both					1252:1255	both	1252:1255	both	1252:1255	The swelling and drug release behaviors revealed that the swelling and drug release rate of ALG@CMC beads were obviously slower than that of simple-ALG and both have significant pH responsiveness.
33345720	6	10	contain	have	1257:1260	arg2	responsiveness					1277:1290	significant pH responsiveness	1262:1290	significant pH responsiveness	1262:1290	The swelling and drug release behaviors revealed that the swelling and drug release rate of ALG@CMC beads were obviously slower than that of simple-ALG and both have significant pH responsiveness.
33345720	0	11	theme	drug	124:127	arg1	delivery					129:136	pH responsive drug delivery	110:136	pH responsive drug delivery	110:136	A core-shell structured alginate hydrogel beads with tunable thickness of carboxymethyl cellulose coating for pH responsive drug delivery.
33345720	3	12	dep	thermo-gravimetric	570:587	arg1	TG					590:591	TG	590:591	TG	590:591	The resulting gel samples were characterized by Fourier transforms infrared (FTIR) spectroscopy, thermo-gravimetric (TG) analysis, and scanning electron microscopy (SEM).
33345720	5	13	theme	mechanical	1002:1011	arg1	stability					1013:1021	the mechanical stability	998:1021	the mechanical stability of ALG@CMC beads	998:1038	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	1	14	theme	ALG	225:227	arg1	core					230:233	a alginate (ALG) core	213:233	a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC)	213:291	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	5	15	theme	37 °C.	955:960	arg1	experiments					974:984	37 °C. Oscillation experiments	955:984	37 °C. Oscillation experiments	955:984	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	7	16	theme	intestinal	1412:1421	arg1	fluid					1423:1427	simulated intestinal fluid	1402:1427	simulated intestinal fluid of pH 7.4	1402:1437	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	6	17	theme	release	1118:1124	arg1	behaviors					1126:1134	The swelling and drug release behaviors	1096:1134	The swelling and drug release behaviors	1096:1134	The swelling and drug release behaviors revealed that the swelling and drug release rate of ALG@CMC beads were obviously slower than that of simple-ALG and both have significant pH responsiveness.
33345720	6	18	theme	CMC	1192:1194	arg1	beads					1196:1200	ALG@CMC beads	1188:1200	ALG@CMC beads	1188:1200	The swelling and drug release behaviors revealed that the swelling and drug release rate of ALG@CMC beads were obviously slower than that of simple-ALG and both have significant pH responsiveness.
33345720	8	19	theme	developed	1561:1569	arg1	device					1644:1649	a pH-responsive drug delivery device in vivo	1614:1657	a pH-responsive drug delivery device in vivo	1614:1657	These developed materials could potentially be employed as a pH-responsive drug delivery device in vivo.
33345720	8	19	theme	developed	1561:1569	arg1	materials					1571:1579	These developed materials	1555:1579	These developed materials	1555:1579	These developed materials could potentially be employed as a pH-responsive drug delivery device in vivo.
33345720	1	20	theme	pH-responsive	139:151	arg1	beads					194:198	pH-responsive core-shell structured composite hydrogel beads	139:198	pH-responsive core-shell structured composite hydrogel beads	139:198	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	7	21	theme	gastric	1515:1521	arg1	fluid					1523:1527	gastric fluid	1515:1527	gastric fluid of pH 1.2	1515:1537	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	2	22	theme	model	461:465	arg1	drug					467:470	a model drug	459:470	a model drug	459:470	An anti-inflammatory drug, indomethacin was loaded into the formed hydrogels as a model drug.
33345720	5	23	theme	beads	1034:1038	arg1	stability					1013:1021	the mechanical stability	998:1021	the mechanical stability of ALG@CMC beads	998:1038	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	3	24	theme	thermo-gravimetric	570:587	arg1	analysis					594:601	thermo-gravimetric (TG) analysis	570:601	thermo-gravimetric (TG) analysis	570:601	The resulting gel samples were characterized by Fourier transforms infrared (FTIR) spectroscopy, thermo-gravimetric (TG) analysis, and scanning electron microscopy (SEM).
33345720	3	25	dep	Fourier	521:527	arg1	transforms					529:538	transforms	529:538	transforms infrared (FTIR) spectroscopy, thermo-gravimetric (TG) analysis, and scanning electron microscopy (SEM)	529:641	The resulting gel samples were characterized by Fourier transforms infrared (FTIR) spectroscopy, thermo-gravimetric (TG) analysis, and scanning electron microscopy (SEM).
33345720	0	26	theme	carboxymethyl	74:86	arg1	coating					98:104	carboxymethyl cellulose coating	74:104	carboxymethyl cellulose coating for pH responsive drug delivery	74:136	A core-shell structured alginate hydrogel beads with tunable thickness of carboxymethyl cellulose coating for pH responsive drug delivery.
33345720	1	27	theme	hydrogel	185:192	arg1	beads					194:198	pH-responsive core-shell structured composite hydrogel beads	139:198	pH-responsive core-shell structured composite hydrogel beads	139:198	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	0	28	theme	coating	98:104	arg1	thickness					61:69	tunable thickness	53:69	tunable thickness of carboxymethyl cellulose coating for pH responsive drug delivery	53:136	A core-shell structured alginate hydrogel beads with tunable thickness of carboxymethyl cellulose coating for pH responsive drug delivery.
33345720	1	29	theme	in-situ	317:323	arg1	preparation					329:339	in-situ gel preparation	317:339	in-situ gel preparation technology	317:350	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	7	30	theme	ALG	1331:1333	arg1	CMC-1					1335:1339	ALG@CMC-1	1331:1339	ALG@CMC-1	1331:1339	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	1	31	theme	preparation	329:339	arg1	system					371:376	a drug delivery system	355:376	a drug delivery system	355:376	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	1	31	theme	preparation	329:339	arg1	technology					341:350	in-situ gel preparation technology	317:350	in-situ gel preparation technology	317:350	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	8	32	theme	pH-responsive	1616:1628	arg1	device					1644:1649	a pH-responsive drug delivery device in vivo	1614:1657	a pH-responsive drug delivery device in vivo	1614:1657	These developed materials could potentially be employed as a pH-responsive drug delivery device in vivo.
33345720	8	32	theme	pH-responsive	1616:1628	arg1	materials					1571:1579	These developed materials	1555:1579	These developed materials	1555:1579	These developed materials could potentially be employed as a pH-responsive drug delivery device in vivo.
33345720	5	33	theme	pH	915:916	arg1	fluid					906:910	gastric fluid	898:910	gastric fluid of pH 1.2	898:920	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	5	33	theme	pH	915:916	arg1	fluid					936:940	intestinal fluid	925:940	intestinal fluid of pH 7.4	925:950	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	5	34	theme	shell	1083:1087	arg1	layer					1089:1093	the CMC shell layer	1075:1093	the CMC shell layer	1075:1093	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	8	35	theme	delivery	1635:1642	arg1	device					1644:1649	a pH-responsive drug delivery device in vivo	1614:1657	a pH-responsive drug delivery device in vivo	1614:1657	These developed materials could potentially be employed as a pH-responsive drug delivery device in vivo.
33345720	8	35	theme	delivery	1635:1642	arg1	materials					1571:1579	These developed materials	1555:1579	These developed materials	1555:1579	These developed materials could potentially be employed as a pH-responsive drug delivery device in vivo.
33345720	4	36	theme	buffered	697:704	arg1	solution					706:713	phosphate buffered solution	687:713	phosphate buffered solution (PBS, pH 7.4)	687:727	The mechanical stability of all samples in phosphate buffered solution (PBS, pH 7.4) was approximately measured through oscillation experiments.
33345720	2	37	theme	anti-inflammatory	382:398	arg1	indomethacin					406:417	indomethacin	406:417	indomethacin	406:417	An anti-inflammatory drug, indomethacin was loaded into the formed hydrogels as a model drug.
33345720	2	37	theme	anti-inflammatory	382:398	arg1	drug					400:403	An anti-inflammatory drug	379:403	An anti-inflammatory drug	379:403	An anti-inflammatory drug, indomethacin was loaded into the formed hydrogels as a model drug.
33345720	3	38	dep	infrared	540:547	arg1	FTIR					550:553	FTIR	550:553	FTIR	550:553	The resulting gel samples were characterized by Fourier transforms infrared (FTIR) spectroscopy, thermo-gravimetric (TG) analysis, and scanning electron microscopy (SEM).
33345720	2	39	theme	formed	439:444	arg1	hydrogels					446:454	the formed hydrogels	435:454	the formed hydrogels as a model drug	435:470	An anti-inflammatory drug, indomethacin was loaded into the formed hydrogels as a model drug.
33345720	0	40	theme	hydrogel	33:40	arg1	beads					42:46	alginate hydrogel beads	24:46	alginate hydrogel beads with tunable thickness of carboxymethyl cellulose coating for pH responsive drug delivery	24:136	A core-shell structured alginate hydrogel beads with tunable thickness of carboxymethyl cellulose coating for pH responsive drug delivery.
33345720	1	41	theme	@	287:287	arg1	CMC					288:290	ALG@CMC	284:290	ALG@CMC	284:290	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	1	41	theme	@	287:287	arg1	shell					277:281	carboxymethyl cellulose (CMC) shell	247:281	carboxymethyl cellulose (CMC) shell (ALG@CMC)	247:291	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	7	42	from	%	1389:1389	arg1	fluid					1423:1427	simulated intestinal fluid	1402:1427	simulated intestinal fluid of pH 7.4	1402:1437	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	5	43	theme	pH	945:946	arg1	fluid					906:910	gastric fluid	898:910	gastric fluid of pH 1.2	898:920	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	5	43	theme	pH	945:946	arg1	fluid					936:940	intestinal fluid	925:940	intestinal fluid of pH 7.4	925:950	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	4	44	theme	oscillation	764:774	arg1	experiments					776:786	oscillation experiments	764:786	oscillation experiments	764:786	The mechanical stability of all samples in phosphate buffered solution (PBS, pH 7.4) was approximately measured through oscillation experiments.
33345720	4	45	dep	solution	706:713	arg1	pH					721:722	pH 7.4	721:726	pH 7.4	721:726	The mechanical stability of all samples in phosphate buffered solution (PBS, pH 7.4) was approximately measured through oscillation experiments.
33345720	4	45	dep	solution	706:713	arg1	PBS					716:718	PBS	716:718	PBS	716:718	The mechanical stability of all samples in phosphate buffered solution (PBS, pH 7.4) was approximately measured through oscillation experiments.
33345720	7	46	theme	@	1359:1359	arg1	CMC-3					1360:1364	@CMC-2 and ALG@CMC-3	1345:1364	CMC-3	1360:1364	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	5	47	theme	ALG	839:841	arg1	beads					847:851	ALG@CMC beads	839:851	ALG@CMC beads compared with ALG	839:869	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	3	48	theme	electron	617:624	arg1	SEM					638:640	SEM	638:640	SEM	638:640	The resulting gel samples were characterized by Fourier transforms infrared (FTIR) spectroscopy, thermo-gravimetric (TG) analysis, and scanning electron microscopy (SEM).
33345720	3	48	theme	electron	617:624	arg1	microscopy					626:635	scanning electron microscopy	608:635	scanning electron microscopy (SEM)	608:641	The resulting gel samples were characterized by Fourier transforms infrared (FTIR) spectroscopy, thermo-gravimetric (TG) analysis, and scanning electron microscopy (SEM).
33345720	6	49	theme	pH	1274:1275	arg1	responsiveness					1277:1290	significant pH responsiveness	1262:1290	significant pH responsiveness	1262:1290	The swelling and drug release behaviors revealed that the swelling and drug release rate of ALG@CMC beads were obviously slower than that of simple-ALG and both have significant pH responsiveness.
33345720	6	50	theme	@	1191:1191	arg1	beads					1196:1200	ALG@CMC beads	1188:1200	ALG@CMC beads	1188:1200	The swelling and drug release behaviors revealed that the swelling and drug release rate of ALG@CMC beads were obviously slower than that of simple-ALG and both have significant pH responsiveness.
33345720	1	51	theme	carboxymethyl	247:259	arg1	CMC					288:290	ALG@CMC	284:290	ALG@CMC	284:290	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	1	51	theme	carboxymethyl	247:259	arg1	shell					277:281	carboxymethyl cellulose (CMC) shell	247:281	carboxymethyl cellulose (CMC) shell (ALG@CMC)	247:291	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	7	52	theme	@	1345:1345	arg1	CMC-2					1346:1350	@CMC-2 and ALG@CMC-3	1345:1364	CMC-2	1346:1350	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	7	53	from	%	1379:1379	arg1	fluid					1423:1427	simulated intestinal fluid	1402:1427	simulated intestinal fluid of pH 7.4	1402:1437	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	5	54	theme	@	842:842	arg1	beads					847:851	ALG@CMC beads	839:851	ALG@CMC beads compared with ALG	839:869	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	6	55	theme	release	1172:1178	arg1	slower					1217:1222	slower	1217:1222	slower	1217:1222	The swelling and drug release behaviors revealed that the swelling and drug release rate of ALG@CMC beads were obviously slower than that of simple-ALG and both have significant pH responsiveness.
33345720	6	55	theme	release	1172:1178	arg1	rate					1180:1183	the swelling and drug release rate	1150:1183	the swelling and drug release rate of ALG@CMC beads	1150:1200	The swelling and drug release behaviors revealed that the swelling and drug release rate of ALG@CMC beads were obviously slower than that of simple-ALG and both have significant pH responsiveness.
33345720	3	56	theme	gel	487:489	arg1	samples					491:497	The resulting gel samples	473:497	The resulting gel samples	473:497	The resulting gel samples were characterized by Fourier transforms infrared (FTIR) spectroscopy, thermo-gravimetric (TG) analysis, and scanning electron microscopy (SEM).
33345720	5	57	theme	controlled	802:811	arg1	release					818:824	controlled drug release	802:824	controlled drug release	802:824	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	7	58	theme	@	1334:1334	arg1	CMC-1					1335:1339	ALG@CMC-1	1331:1339	ALG@CMC-1	1331:1339	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	6	59	theme	significant	1262:1272	arg1	responsiveness					1277:1290	significant pH responsiveness	1262:1290	significant pH responsiveness	1262:1290	The swelling and drug release behaviors revealed that the swelling and drug release rate of ALG@CMC beads were obviously slower than that of simple-ALG and both have significant pH responsiveness.
33345720	5	60	theme	release	818:824	arg1	behaviors					826:834	Swelling and controlled drug release behaviors	789:834	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG	789:869	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	7	61	from	CMC-1	1335:1339	arg1	%					1379:1379	about 100%	1370:1379	about 100%	1370:1379	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	7	61	from	CMC-1	1335:1339	arg1	release					1313:1319	The cumulative drug release	1293:1319	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3	1293:1364	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	7	62	theme	pH	1432:1433	arg1	fluid					1423:1427	simulated intestinal fluid	1402:1427	simulated intestinal fluid of pH 7.4	1402:1437	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	5	63	theme	CMC	843:845	arg1	beads					847:851	ALG@CMC beads	839:851	ALG@CMC beads compared with ALG	839:869	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	5	64	theme	Swelling	789:796	arg1	behaviors					826:834	Swelling and controlled drug release behaviors	789:834	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG	789:869	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	7	65	from	CMC-2	1346:1350	arg1	%					1379:1379	about 100%	1370:1379	about 100%	1370:1379	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	7	65	from	CMC-2	1346:1350	arg1	release					1313:1319	The cumulative drug release	1293:1319	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3	1293:1364	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	1	66	theme	delivery	362:369	arg1	system					371:376	a drug delivery system	355:376	a drug delivery system	355:376	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	1	66	theme	delivery	362:369	arg1	technology					341:350	in-situ gel preparation technology	317:350	in-situ gel preparation technology	317:350	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	5	67	theme	beads	847:851	arg1	behaviors					826:834	Swelling and controlled drug release behaviors	789:834	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG	789:869	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	7	68	theme	simulated	1402:1410	arg1	fluid					1423:1427	simulated intestinal fluid	1402:1427	simulated intestinal fluid of pH 7.4	1402:1437	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	4	69	theme	mechanical	648:657	arg1	stability					659:667	The mechanical stability	644:667	The mechanical stability of all samples in phosphate buffered solution (PBS, pH 7.4)	644:727	The mechanical stability of all samples in phosphate buffered solution (PBS, pH 7.4) was approximately measured through oscillation experiments.
33345720	6	70	theme	beads	1196:1200	arg1	slower					1217:1222	slower	1217:1222	slower	1217:1222	The swelling and drug release behaviors revealed that the swelling and drug release rate of ALG@CMC beads were obviously slower than that of simple-ALG and both have significant pH responsiveness.
33345720	6	70	theme	beads	1196:1200	arg1	rate					1180:1183	the swelling and drug release rate	1150:1183	the swelling and drug release rate of ALG@CMC beads	1150:1200	The swelling and drug release behaviors revealed that the swelling and drug release rate of ALG@CMC beads were obviously slower than that of simple-ALG and both have significant pH responsiveness.
33345720	5	71	theme	Oscillation	962:972	arg1	experiments					974:984	37 °C. Oscillation experiments	955:984	37 °C. Oscillation experiments	955:984	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	7	72	theme	pH	1532:1533	arg1	fluid					1523:1527	gastric fluid	1515:1527	gastric fluid of pH 1.2	1515:1537	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	6	73	theme	drug	1113:1116	arg1	release					1118:1124	drug release	1113:1124	drug release	1113:1124	The swelling and drug release behaviors revealed that the swelling and drug release rate of ALG@CMC beads were obviously slower than that of simple-ALG and both have significant pH responsiveness.
33345720	1	74	theme	core-shell	153:162	arg1	beads					194:198	pH-responsive core-shell structured composite hydrogel beads	139:198	pH-responsive core-shell structured composite hydrogel beads	139:198	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	6	75	theme	swelling	1100:1107	arg1	behaviors					1126:1134	The swelling and drug release behaviors	1096:1134	The swelling and drug release behaviors	1096:1134	The swelling and drug release behaviors revealed that the swelling and drug release rate of ALG@CMC beads were obviously slower than that of simple-ALG and both have significant pH responsiveness.
33345720	0	76	theme	cellulose	88:96	arg1	coating					98:104	carboxymethyl cellulose coating	74:104	carboxymethyl cellulose coating for pH responsive drug delivery	74:136	A core-shell structured alginate hydrogel beads with tunable thickness of carboxymethyl cellulose coating for pH responsive drug delivery.
33345720	1	77	theme	composite	175:183	arg1	beads					194:198	pH-responsive core-shell structured composite hydrogel beads	139:198	pH-responsive core-shell structured composite hydrogel beads	139:198	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	7	78	from	ALG	1326:1328	arg1	%					1379:1379	about 100%	1370:1379	about 100%	1370:1379	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	7	78	from	ALG	1326:1328	arg1	release					1313:1319	The cumulative drug release	1293:1319	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3	1293:1364	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	9	79	dep	[Formula	1659:1666	arg1	see					1669:1671	see	1669:1671	see text	1669:1676	[Formula: see text].
33345720	5	80	theme	gastric	898:904	arg1	fluid					906:910	gastric fluid	898:910	gastric fluid of pH 1.2	898:920	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	7	81	theme	cumulative	1297:1306	arg1	%					1379:1379	about 100%	1370:1379	about 100%	1370:1379	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	7	81	theme	cumulative	1297:1306	arg1	release					1313:1319	The cumulative drug release	1293:1319	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3	1293:1364	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	0	82	theme	responsive	113:122	arg1	delivery					129:136	pH responsive drug delivery	110:136	pH responsive drug delivery	110:136	A core-shell structured alginate hydrogel beads with tunable thickness of carboxymethyl cellulose coating for pH responsive drug delivery.
33345720	1	83	theme	gel	325:327	arg1	preparation					329:339	in-situ gel preparation	317:339	in-situ gel preparation technology	317:350	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	1	84	theme	alginate	215:222	arg1	core					230:233	a alginate (ALG) core	213:233	a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC)	213:291	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	5	85	theme	CMC	1030:1032	arg1	beads					1034:1038	ALG@CMC beads	1026:1038	ALG@CMC beads	1026:1038	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	5	86	theme	ALG	1026:1028	arg1	beads					1034:1038	ALG@CMC beads	1026:1038	ALG@CMC beads	1026:1038	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	4	87	theme	samples	676:682	arg1	stability					659:667	The mechanical stability	644:667	The mechanical stability of all samples in phosphate buffered solution (PBS, pH 7.4)	644:727	The mechanical stability of all samples in phosphate buffered solution (PBS, pH 7.4) was approximately measured through oscillation experiments.
33345720	0	88	theme	alginate	24:31	arg1	beads					42:46	alginate hydrogel beads	24:46	alginate hydrogel beads with tunable thickness of carboxymethyl cellulose coating for pH responsive drug delivery	24:136	A core-shell structured alginate hydrogel beads with tunable thickness of carboxymethyl cellulose coating for pH responsive drug delivery.
33345720	7	89	from	%	1384:1384	arg1	fluid					1423:1427	simulated intestinal fluid	1402:1427	simulated intestinal fluid of pH 7.4	1402:1437	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	8	90	theme	drug	1630:1633	arg1	device					1644:1649	a pH-responsive drug delivery device in vivo	1614:1657	a pH-responsive drug delivery device in vivo	1614:1657	These developed materials could potentially be employed as a pH-responsive drug delivery device in vivo.
33345720	8	90	theme	drug	1630:1633	arg1	materials					1571:1579	These developed materials	1555:1579	These developed materials	1555:1579	These developed materials could potentially be employed as a pH-responsive drug delivery device in vivo.
33345720	7	91	theme	drug	1464:1467	arg1	release					1469:1475	the drug release	1460:1475	the drug release	1460:1475	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	1	92	theme	ALG	284:286	arg1	CMC					288:290	ALG@CMC	284:290	ALG@CMC	284:290	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	1	92	theme	ALG	284:286	arg1	shell					277:281	carboxymethyl cellulose (CMC) shell	247:281	carboxymethyl cellulose (CMC) shell (ALG@CMC)	247:291	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	0	93	theme	tunable	53:59	arg1	thickness					61:69	tunable thickness	53:69	tunable thickness of carboxymethyl cellulose coating for pH responsive drug delivery	53:136	A core-shell structured alginate hydrogel beads with tunable thickness of carboxymethyl cellulose coating for pH responsive drug delivery.
33345720	5	94	theme	@	1029:1029	arg1	beads					1034:1038	ALG@CMC beads	1026:1038	ALG@CMC beads	1026:1038	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	1	95	theme	structured	164:173	arg1	beads					194:198	pH-responsive core-shell structured composite hydrogel beads	139:198	pH-responsive core-shell structured composite hydrogel beads	139:198	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	1	96	theme	drug	357:360	arg1	system					371:376	a drug delivery system	355:376	a drug delivery system	355:376	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	1	96	theme	drug	357:360	arg1	technology					341:350	in-situ gel preparation technology	317:350	in-situ gel preparation technology	317:350	pH-responsive core-shell structured composite hydrogel beads, composed of a alginate (ALG) core coated with carboxymethyl cellulose (CMC) shell (ALG@CMC), were prepared by using in-situ gel preparation technology as a drug delivery system.
33345720	5	97	theme	CMC	1079:1081	arg1	layer					1089:1093	the CMC shell layer	1075:1093	the CMC shell layer	1075:1093	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	7	98	from	%	1397:1397	arg1	fluid					1423:1427	simulated intestinal fluid	1402:1427	simulated intestinal fluid of pH 7.4	1402:1437	The cumulative drug release from ALG, ALG@CMC-1, ALG@CMC-2 and ALG@CMC-3 was about 100%, 67%, 46% and 37% in simulated intestinal fluid of pH 7.4, respectively, while the drug release reached only about 2.0% in simulating gastric fluid of pH 1.2 within 720 min.
33345720	3	99	theme	scanning	608:615	arg1	SEM					638:640	SEM	638:640	SEM	638:640	The resulting gel samples were characterized by Fourier transforms infrared (FTIR) spectroscopy, thermo-gravimetric (TG) analysis, and scanning electron microscopy (SEM).
33345720	3	99	theme	scanning	608:615	arg1	microscopy					626:635	scanning electron microscopy	608:635	scanning electron microscopy (SEM)	608:641	The resulting gel samples were characterized by Fourier transforms infrared (FTIR) spectroscopy, thermo-gravimetric (TG) analysis, and scanning electron microscopy (SEM).
33345720	4	100	from	stability	659:667	arg1	solution					706:713	phosphate buffered solution	687:713	phosphate buffered solution (PBS, pH 7.4)	687:727	The mechanical stability of all samples in phosphate buffered solution (PBS, pH 7.4) was approximately measured through oscillation experiments.
33345720	5	101	theme	intestinal	925:934	arg1	fluid					936:940	intestinal fluid	925:940	intestinal fluid of pH 7.4	925:950	Swelling and controlled drug release behaviors of ALG@CMC beads compared with ALG were studied in simulating gastric fluid of pH 1.2 or intestinal fluid of pH 7.4 at 37 °C. Oscillation experiments proved that the mechanical stability of ALG@CMC beads could be significantly improved by the CMC shell layer.
33345720	0	102	with	beads	42:46	arg1	thickness					61:69	tunable thickness	53:69	tunable thickness of carboxymethyl cellulose coating for pH responsive drug delivery	53:136	A core-shell structured alginate hydrogel beads with tunable thickness of carboxymethyl cellulose coating for pH responsive drug delivery.
33345720	3	103	theme	resulting	477:485	arg1	samples					491:497	The resulting gel samples	473:497	The resulting gel samples	473:497	The resulting gel samples were characterized by Fourier transforms infrared (FTIR) spectroscopy, thermo-gravimetric (TG) analysis, and scanning electron microscopy (SEM).
33223210	2	0	theme	cell	534:537	arg1	peroxidase					544:553	cell wall peroxidase	534:553	cell wall peroxidase	534:553	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	10	1	from	immobilization	1396:1409	arg1	wall					1423:1426	the cell wall	1414:1426	the cell wall	1414:1426	Taken together, the results show that AM inoculation may improve host plant growth and increase Pb immobilization in the cell wall by increasing the polysaccharide content within pectin and hemicellulose and by inducing cell wall peroxidase activity.
33223210	10	2	theme	cell	1517:1520	arg1	activity					1538:1545	cell wall peroxidase activity	1517:1545	cell wall peroxidase activity	1517:1545	Taken together, the results show that AM inoculation may improve host plant growth and increase Pb immobilization in the cell wall by increasing the polysaccharide content within pectin and hemicellulose and by inducing cell wall peroxidase activity.
33223210	1	3	theme	cell	245:248	arg1	wall					250:253	the cell wall	241:253	the cell wall	241:253	The mechanism by which arbuscular mycorrhizal (AM) fungi immobilize lead (Pb) within the cell wall is unclear.
33223210	10	4	theme	host	1362:1365	arg1	growth					1373:1378	host plant growth	1362:1378	host plant growth	1362:1378	Taken together, the results show that AM inoculation may improve host plant growth and increase Pb immobilization in the cell wall by increasing the polysaccharide content within pectin and hemicellulose and by inducing cell wall peroxidase activity.
33223210	11	5	theme	mycorrhizal-associated	1659:1680	arg1	plants					1682:1687	mycorrhizal-associated plants	1659:1687	mycorrhizal-associated plants	1659:1687	Both the polysaccharide composition and cell wall peroxidase have important contributions to the resistance of mycorrhizal-associated plants.
33223210	1	6	theme	mycorrhizal	190:200	arg1	fungi					207:211	arbuscular mycorrhizal (AM) fungi	179:211	arbuscular mycorrhizal (AM) fungi	179:211	The mechanism by which arbuscular mycorrhizal (AM) fungi immobilize lead (Pb) within the cell wall is unclear.
33223210	8	7	theme	polysaccharide	1150:1163	arg1	cross-linking					1165:1177	polysaccharide cross-linking	1150:1177	polysaccharide cross-linking	1150:1177	Correlation analysis suggested that MtPrx05 and MtPrx10 may participate in polysaccharide cross-linking and cell wall stiffening.
33223210	2	8	theme	peroxidase	544:553	arg1	contents					471:478	the uronic acid contents	455:478	the uronic acid contents	455:478	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	2	8	theme	peroxidase	544:553	arg1	polysaccharide					436:449	polysaccharide	436:449	polysaccharide	436:449	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	2	8	theme	peroxidase	544:553	arg1	activity					522:529	the activity	518:529	the activity of cell wall peroxidase	518:553	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	2	8	theme	peroxidase	544:553	arg1	content					406:412	the Pb content	399:412	the Pb content in the cell wall	399:429	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	7	9	theme	peroxidase	1027:1036	arg1	activity					1038:1045	the cell wall peroxidase activity	1013:1045	the cell wall peroxidase activity in non-AM-associated roots	1013:1072	However, Pb stress did not affect the cell wall peroxidase activity in non-AM-associated roots.
33223210	1	10	theme	AM	203:204	arg1	fungi					207:211	arbuscular mycorrhizal (AM) fungi	179:211	arbuscular mycorrhizal (AM) fungi	179:211	The mechanism by which arbuscular mycorrhizal (AM) fungi immobilize lead (Pb) within the cell wall is unclear.
33223210	6	11	theme	peroxidase	923:932	arg1	activity					934:941	the cell wall peroxidase activity	909:941	the cell wall peroxidase activity	909:941	In AM-associated roots, the cell wall peroxidase activity increased in response to Pb stress.
33223210	2	12	theme	cell	493:496	arg1	fractions					503:511	different cell wall fractions	483:511	different cell wall fractions	483:511	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	7	13	theme	cell	1017:1020	arg1	activity					1038:1045	the cell wall peroxidase activity	1013:1045	the cell wall peroxidase activity in non-AM-associated roots	1013:1072	However, Pb stress did not affect the cell wall peroxidase activity in non-AM-associated roots.
33223210	8	14	theme	wall	1188:1191	arg1	stiffening					1193:1202	cell wall stiffening	1183:1202	cell wall stiffening	1183:1202	Correlation analysis suggested that MtPrx05 and MtPrx10 may participate in polysaccharide cross-linking and cell wall stiffening.
33223210	6	15	theme	cell	913:916	arg1	peroxidase					923:932	cell wall peroxidase	913:932	the cell wall peroxidase activity	909:941	In AM-associated roots, the cell wall peroxidase activity increased in response to Pb stress.
33223210	9	16	theme	stress	1212:1217	arg1	mechanism					1230:1238	The Pb stress resistance mechanism	1205:1238	The Pb stress resistance mechanism of AM-associated roots	1205:1261	The Pb stress resistance mechanism of AM-associated roots may involve cell wall stiffening.
33223210	6	17	theme	AM-associated	888:900	arg1	roots					902:906	AM-associated roots	888:906	AM-associated roots	888:906	In AM-associated roots, the cell wall peroxidase activity increased in response to Pb stress.
33223210	2	18	theme	fractions	503:511	arg1	contents					471:478	the uronic acid contents	455:478	the uronic acid contents	455:478	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	2	18	theme	fractions	503:511	arg1	polysaccharide					436:449	polysaccharide	436:449	polysaccharide	436:449	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	2	18	theme	fractions	503:511	arg1	activity					522:529	the activity	518:529	the activity of cell wall peroxidase	518:553	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	2	18	theme	fractions	503:511	arg1	content					406:412	the Pb content	399:412	the Pb content in the cell wall	399:429	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	7	19	theme	Pb	988:989	arg1	stress					991:996	Pb stress	988:996	Pb stress	988:996	However, Pb stress did not affect the cell wall peroxidase activity in non-AM-associated roots.
33223210	9	20	theme	AM-associated	1243:1255	arg1	roots					1257:1261	AM-associated roots	1243:1261	AM-associated roots	1243:1261	The Pb stress resistance mechanism of AM-associated roots may involve cell wall stiffening.
33223210	3	21	theme	Mycorrhizal-associated	556:577	arg1	truncatula					588:597	Mycorrhizal-associated Medicago truncatula	556:597	Mycorrhizal-associated Medicago truncatula	556:597	Mycorrhizal-associated Medicago truncatula had higher shoot and root biomass than nonmycorrhizal-associated M. truncatula.
33223210	6	22	theme	Pb	968:969	arg1	stress					971:976	Pb stress	968:976	Pb stress	968:976	In AM-associated roots, the cell wall peroxidase activity increased in response to Pb stress.
33223210	9	23	theme	cell	1275:1278	arg1	stiffening					1285:1294	cell wall stiffening	1275:1294	cell wall stiffening	1275:1294	The Pb stress resistance mechanism of AM-associated roots may involve cell wall stiffening.
33223210	2	24	theme	acid	466:469	arg1	contents					471:478	the uronic acid contents	455:478	the uronic acid contents	455:478	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	4	25	theme	cell	729:732	arg1	wall					734:737	the cell wall	725:737	the cell wall	725:737	AM inoculation increased the content of Pb in the cell wall under Pb stress.
33223210	2	26	from	content	406:412	arg1	wall					426:429	the cell wall	417:429	the cell wall	417:429	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	10	27	theme	polysaccharide	1446:1459	arg1	content					1461:1467	the polysaccharide content	1442:1467	the polysaccharide content	1442:1467	Taken together, the results show that AM inoculation may improve host plant growth and increase Pb immobilization in the cell wall by increasing the polysaccharide content within pectin and hemicellulose and by inducing cell wall peroxidase activity.
33223210	2	28	theme	Pb	403:404	arg1	content					406:412	the Pb content	399:412	the Pb content in the cell wall	399:429	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	0	29	theme	Arbuscular	0:9	arg1	fungi					23:27	Arbuscular mycorrhizal fungi	0:27	Arbuscular mycorrhizal fungi	0:27	Arbuscular mycorrhizal fungi promote lead immobilization by increasing the polysaccharide content within pectin and inducing cell wall peroxidase activity.
33223210	11	30	theme	polysaccharide	1557:1570	arg1	composition					1572:1582	the polysaccharide composition	1553:1582	the polysaccharide composition	1553:1582	Both the polysaccharide composition and cell wall peroxidase have important contributions to the resistance of mycorrhizal-associated plants.
33223210	2	31	theme	cell	421:424	arg1	wall					426:429	the cell wall	417:429	the cell wall	417:429	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	3	32	theme	higher	603:608	arg1	shoot					610:614	higher shoot	603:614	higher shoot	603:614	Mycorrhizal-associated Medicago truncatula had higher shoot and root biomass than nonmycorrhizal-associated M. truncatula.
33223210	0	33	theme	lead	37:40	arg1	immobilization					42:55	lead immobilization	37:55	lead immobilization	37:55	Arbuscular mycorrhizal fungi promote lead immobilization by increasing the polysaccharide content within pectin and inducing cell wall peroxidase activity.
33223210	11	34	theme	wall	1593:1596	arg1	peroxidase					1598:1607	cell wall peroxidase	1588:1607	cell wall peroxidase	1588:1607	Both the polysaccharide composition and cell wall peroxidase have important contributions to the resistance of mycorrhizal-associated plants.
33223210	2	35	from	contents	471:478	arg1	wall					426:429	the cell wall	417:429	the cell wall	417:429	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	10	36	theme	wall	1522:1525	arg1	activity					1538:1545	cell wall peroxidase activity	1517:1545	cell wall peroxidase activity	1517:1545	Taken together, the results show that AM inoculation may improve host plant growth and increase Pb immobilization in the cell wall by increasing the polysaccharide content within pectin and hemicellulose and by inducing cell wall peroxidase activity.
33223210	3	37	theme	nonmycorrhizal-associated	638:662	arg1	M. truncatula					664:676	nonmycorrhizal-associated M. truncatula	638:676	nonmycorrhizal-associated M. truncatula	638:676	Mycorrhizal-associated Medicago truncatula had higher shoot and root biomass than nonmycorrhizal-associated M. truncatula.
33223210	7	38	theme	non-AM-associated	1050:1066	arg1	roots					1068:1072	non-AM-associated roots	1050:1072	non-AM-associated roots	1050:1072	However, Pb stress did not affect the cell wall peroxidase activity in non-AM-associated roots.
33223210	10	39	theme	plant	1367:1371	arg1	growth					1373:1378	host plant growth	1362:1378	host plant growth	1362:1378	Taken together, the results show that AM inoculation may improve host plant growth and increase Pb immobilization in the cell wall by increasing the polysaccharide content within pectin and hemicellulose and by inducing cell wall peroxidase activity.
33223210	1	40	theme	arbuscular	179:188	arg1	fungi					207:211	arbuscular mycorrhizal (AM) fungi	179:211	arbuscular mycorrhizal (AM) fungi	179:211	The mechanism by which arbuscular mycorrhizal (AM) fungi immobilize lead (Pb) within the cell wall is unclear.
33223210	11	41	theme	plants	1682:1687	arg1	resistance					1645:1654	the resistance	1641:1654	the resistance of mycorrhizal-associated plants	1641:1687	Both the polysaccharide composition and cell wall peroxidase have important contributions to the resistance of mycorrhizal-associated plants.
33223210	7	42	theme	wall	1022:1025	arg1	activity					1038:1045	the cell wall peroxidase activity	1013:1045	the cell wall peroxidase activity in non-AM-associated roots	1013:1072	However, Pb stress did not affect the cell wall peroxidase activity in non-AM-associated roots.
33223210	10	43	theme	Pb	1393:1394	arg1	immobilization					1396:1409	Pb immobilization	1393:1409	Pb immobilization in the cell wall by increasing the polysaccharide content within pectin and hemicellulose and by inducing cell wall peroxidase activity	1393:1545	Taken together, the results show that AM inoculation may improve host plant growth and increase Pb immobilization in the cell wall by increasing the polysaccharide content within pectin and hemicellulose and by inducing cell wall peroxidase activity.
33223210	11	44	contain	have	1609:1612	arg2	contributions					1624:1636	important contributions	1614:1636	important contributions	1614:1636	Both the polysaccharide composition and cell wall peroxidase have important contributions to the resistance of mycorrhizal-associated plants.
33223210	11	44	contain	have	1609:1612	arg1	composition					1572:1582	the polysaccharide composition	1553:1582	the polysaccharide composition	1553:1582	Both the polysaccharide composition and cell wall peroxidase have important contributions to the resistance of mycorrhizal-associated plants.
33223210	11	44	contain	have	1609:1612	arg1	peroxidase					1598:1607	cell wall peroxidase	1588:1607	cell wall peroxidase	1588:1607	Both the polysaccharide composition and cell wall peroxidase have important contributions to the resistance of mycorrhizal-associated plants.
33223210	2	45	theme	wall	539:542	arg1	peroxidase					544:553	cell wall peroxidase	534:553	cell wall peroxidase	534:553	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	0	46	theme	peroxidase	135:144	arg1	activity					146:153	cell wall peroxidase activity	125:153	cell wall peroxidase activity	125:153	Arbuscular mycorrhizal fungi promote lead immobilization by increasing the polysaccharide content within pectin and inducing cell wall peroxidase activity.
33223210	5	47	theme	pectin	790:795	arg1	fractions					815:823	the pectin and hemicellulose fractions	786:823	fractions	815:823	The polysaccharide content in the pectin and hemicellulose fractions were increased by AM inoculation with or without Pb stress.
33223210	6	48	theme	wall	918:921	arg1	peroxidase					923:932	cell wall peroxidase	913:932	the cell wall peroxidase activity	909:941	In AM-associated roots, the cell wall peroxidase activity increased in response to Pb stress.
33223210	2	49	theme	different	483:491	arg1	fractions					503:511	different cell wall fractions	483:511	different cell wall fractions	483:511	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	9	50	theme	Pb	1209:1210	arg1	mechanism					1230:1238	The Pb stress resistance mechanism	1205:1238	The Pb stress resistance mechanism of AM-associated roots	1205:1261	The Pb stress resistance mechanism of AM-associated roots may involve cell wall stiffening.
33223210	5	51	theme	hemicellulose	801:813	arg1	fractions					815:823	the pectin and hemicellulose fractions	786:823	fractions	815:823	The polysaccharide content in the pectin and hemicellulose fractions were increased by AM inoculation with or without Pb stress.
33223210	8	52	theme	cell	1183:1186	arg1	stiffening					1193:1202	cell wall stiffening	1183:1202	cell wall stiffening	1183:1202	Correlation analysis suggested that MtPrx05 and MtPrx10 may participate in polysaccharide cross-linking and cell wall stiffening.
33223210	2	53	from	polysaccharide	436:449	arg1	wall					426:429	the cell wall	417:429	the cell wall	417:429	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	3	54	contain	had	599:601	arg2	biomass					625:631	root biomass	620:631	root biomass	620:631	Mycorrhizal-associated Medicago truncatula had higher shoot and root biomass than nonmycorrhizal-associated M. truncatula.
33223210	3	54	contain	had	599:601	arg1	truncatula					588:597	Mycorrhizal-associated Medicago truncatula	556:597	Mycorrhizal-associated Medicago truncatula	556:597	Mycorrhizal-associated Medicago truncatula had higher shoot and root biomass than nonmycorrhizal-associated M. truncatula.
33223210	3	54	contain	had	599:601	arg2	shoot					610:614	higher shoot	603:614	higher shoot	603:614	Mycorrhizal-associated Medicago truncatula had higher shoot and root biomass than nonmycorrhizal-associated M. truncatula.
33223210	9	55	theme	resistance	1219:1228	arg1	mechanism					1230:1238	The Pb stress resistance mechanism	1205:1238	The Pb stress resistance mechanism of AM-associated roots	1205:1261	The Pb stress resistance mechanism of AM-associated roots may involve cell wall stiffening.
33223210	8	56	theme	Correlation	1075:1085	arg1	analysis					1087:1094	Correlation analysis	1075:1094	Correlation analysis	1075:1094	Correlation analysis suggested that MtPrx05 and MtPrx10 may participate in polysaccharide cross-linking and cell wall stiffening.
33223210	5	57	theme	Pb	874:875	arg1	stress					877:882	Pb stress	874:882	Pb stress	874:882	The polysaccharide content in the pectin and hemicellulose fractions were increased by AM inoculation with or without Pb stress.
33223210	4	58	theme	AM	679:680	arg1	inoculation					682:692	AM inoculation	679:692	AM inoculation	679:692	AM inoculation increased the content of Pb in the cell wall under Pb stress.
33223210	2	59	theme	wall	498:501	arg1	fractions					503:511	different cell wall fractions	483:511	different cell wall fractions	483:511	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	0	60	theme	polysaccharide	75:88	arg1	content					90:96	the polysaccharide content	71:96	the polysaccharide content within pectin	71:110	Arbuscular mycorrhizal fungi promote lead immobilization by increasing the polysaccharide content within pectin and inducing cell wall peroxidase activity.
33223210	9	61	theme	roots	1257:1261	arg1	mechanism					1230:1238	The Pb stress resistance mechanism	1205:1238	The Pb stress resistance mechanism of AM-associated roots	1205:1261	The Pb stress resistance mechanism of AM-associated roots may involve cell wall stiffening.
33223210	0	62	theme	wall	130:133	arg1	activity					146:153	cell wall peroxidase activity	125:153	cell wall peroxidase activity	125:153	Arbuscular mycorrhizal fungi promote lead immobilization by increasing the polysaccharide content within pectin and inducing cell wall peroxidase activity.
33223210	10	63	theme	cell	1418:1421	arg1	wall					1423:1426	the cell wall	1414:1426	the cell wall	1414:1426	Taken together, the results show that AM inoculation may improve host plant growth and increase Pb immobilization in the cell wall by increasing the polysaccharide content within pectin and hemicellulose and by inducing cell wall peroxidase activity.
33223210	9	64	theme	wall	1280:1283	arg1	stiffening					1285:1294	cell wall stiffening	1275:1294	cell wall stiffening	1275:1294	The Pb stress resistance mechanism of AM-associated roots may involve cell wall stiffening.
33223210	2	65	theme	uronic	459:464	arg1	contents					471:478	the uronic acid contents	455:478	the uronic acid contents	455:478	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	0	66	theme	cell	125:128	arg1	activity					146:153	cell wall peroxidase activity	125:153	cell wall peroxidase activity	125:153	Arbuscular mycorrhizal fungi promote lead immobilization by increasing the polysaccharide content within pectin and inducing cell wall peroxidase activity.
33223210	5	67	theme	polysaccharide	760:773	arg1	content					775:781	The polysaccharide content	756:781	The polysaccharide content in the pectin and hemicellulose fractions	756:823	The polysaccharide content in the pectin and hemicellulose fractions were increased by AM inoculation with or without Pb stress.
33223210	5	68	from	content	775:781	arg1	fractions					815:823	the pectin and hemicellulose fractions	786:823	fractions	815:823	The polysaccharide content in the pectin and hemicellulose fractions were increased by AM inoculation with or without Pb stress.
33223210	2	69	from	activity	522:529	arg1	wall					426:429	the cell wall	417:429	the cell wall	417:429	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	2	70	theme	AM	342:343	arg1	fungi					345:349	AM fungi	342:349	AM fungi	342:349	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	4	71	from	content	708:714	arg1	wall					734:737	the cell wall	725:737	the cell wall	725:737	AM inoculation increased the content of Pb in the cell wall under Pb stress.
33223210	7	72	from	activity	1038:1045	arg1	roots					1068:1072	non-AM-associated roots	1050:1072	non-AM-associated roots	1050:1072	However, Pb stress did not affect the cell wall peroxidase activity in non-AM-associated roots.
33223210	4	73	theme	Pb	719:720	arg1	content					708:714	the content	704:714	the content of Pb in the cell wall	704:737	AM inoculation increased the content of Pb in the cell wall under Pb stress.
33223210	2	74	theme	cell	376:379	arg1	wall					381:384	the cell wall	372:384	the cell wall	372:384	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	3	75	theme	Medicago	579:586	arg1	truncatula					588:597	Mycorrhizal-associated Medicago truncatula	556:597	Mycorrhizal-associated Medicago truncatula	556:597	Mycorrhizal-associated Medicago truncatula had higher shoot and root biomass than nonmycorrhizal-associated M. truncatula.
33223210	11	76	theme	cell	1588:1591	arg1	peroxidase					1598:1607	cell wall peroxidase	1588:1607	cell wall peroxidase	1588:1607	Both the polysaccharide composition and cell wall peroxidase have important contributions to the resistance of mycorrhizal-associated plants.
33223210	3	77	theme	root	620:623	arg1	biomass					625:631	root biomass	620:631	root biomass	620:631	Mycorrhizal-associated Medicago truncatula had higher shoot and root biomass than nonmycorrhizal-associated M. truncatula.
33223210	2	78	dep	polysaccharide	436:449	arg1	the					432:434	the	432:434	the	432:434	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	4	79	theme	Pb	745:746	arg1	stress					748:753	Pb stress	745:753	Pb stress	745:753	AM inoculation increased the content of Pb in the cell wall under Pb stress.
33223210	11	80	theme	important	1614:1622	arg1	contributions					1624:1636	important contributions	1614:1636	important contributions	1614:1636	Both the polysaccharide composition and cell wall peroxidase have important contributions to the resistance of mycorrhizal-associated plants.
33223210	2	81	theme	study	294:298	arg1	aim					282:284	the aim	278:284	the aim of this study	278:298	Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase.
33223210	10	82	dep	immobilization	1396:1409	arg1	increasing					1431:1440	increasing	1431:1440	increasing the polysaccharide content within pectin and hemicellulose	1431:1499	Taken together, the results show that AM inoculation may improve host plant growth and increase Pb immobilization in the cell wall by increasing the polysaccharide content within pectin and hemicellulose and by inducing cell wall peroxidase activity.
33223210	10	82	dep	immobilization	1396:1409	arg1	inducing					1508:1515	inducing	1508:1515	inducing cell wall peroxidase activity	1508:1545	Taken together, the results show that AM inoculation may improve host plant growth and increase Pb immobilization in the cell wall by increasing the polysaccharide content within pectin and hemicellulose and by inducing cell wall peroxidase activity.
33223210	10	83	theme	peroxidase	1527:1536	arg1	activity					1538:1545	cell wall peroxidase activity	1517:1545	cell wall peroxidase activity	1517:1545	Taken together, the results show that AM inoculation may improve host plant growth and increase Pb immobilization in the cell wall by increasing the polysaccharide content within pectin and hemicellulose and by inducing cell wall peroxidase activity.
33223210	10	84	theme	AM	1335:1336	arg1	inoculation					1338:1348	AM inoculation	1335:1348	AM inoculation	1335:1348	Taken together, the results show that AM inoculation may improve host plant growth and increase Pb immobilization in the cell wall by increasing the polysaccharide content within pectin and hemicellulose and by inducing cell wall peroxidase activity.
33223210	0	85	theme	mycorrhizal	11:21	arg1	fungi					23:27	Arbuscular mycorrhizal fungi	0:27	Arbuscular mycorrhizal fungi	0:27	Arbuscular mycorrhizal fungi promote lead immobilization by increasing the polysaccharide content within pectin and inducing cell wall peroxidase activity.
34619636	1	0	theme	quasi-static	158:169	arg1	compression					180:190	quasi-static uniaxial compression	158:190	quasi-static uniaxial compression	158:190	Constitutive modeling of solid methyl cellulose (MC) hydrogels under quasi-static uniaxial compression is presented for a variety of compositions and test temperatures.
34619636	2	1	theme	accurate	357:364	arg1	representation					375:388	the simplest accurate material representation	344:388	the simplest accurate material representation	344:388	Five constitutive models of varying complexity are examined, with the aim to identify the simplest accurate material representation.
34619636	1	2	theme	uniaxial	171:178	arg1	compression					180:190	quasi-static uniaxial compression	158:190	quasi-static uniaxial compression	158:190	Constitutive modeling of solid methyl cellulose (MC) hydrogels under quasi-static uniaxial compression is presented for a variety of compositions and test temperatures.
34619636	2	3	theme	material	366:373	arg1	representation					375:388	the simplest accurate material representation	344:388	the simplest accurate material representation	344:388	Five constitutive models of varying complexity are examined, with the aim to identify the simplest accurate material representation.
34619636	4	4	theme	tested	574:579	arg1	models					581:586	all the tested models	566:586	all the tested models	566:586	It is found that of all the tested models, the second order polynomial constitutive model fulfills the requirements of simplicity and accuracy both for compression and predicted tension.
34619636	2	5	theme	simplest	348:355	arg1	representation					375:388	the simplest accurate material representation	344:388	the simplest accurate material representation	344:388	Five constitutive models of varying complexity are examined, with the aim to identify the simplest accurate material representation.
34619636	2	6	theme	complexity	294:303	arg1	models					276:281	Five constitutive models	258:281	Five constitutive models of varying complexity	258:303	Five constitutive models of varying complexity are examined, with the aim to identify the simplest accurate material representation.
34619636	3	7	theme	gel	419:421	arg1	viscosity					402:410	the viscosity	398:410	the viscosity of the gel	398:421	Due to the viscosity of the gel, the models were calibrated using compression tests only, with restrictions that ensure stability for other loading modes.
34619636	1	8	theme	Constitutive	89:100	arg1	modeling					102:109	Constitutive modeling	89:109	Constitutive modeling of solid methyl cellulose (MC) hydrogels under quasi-static uniaxial compression	89:190	Constitutive modeling of solid methyl cellulose (MC) hydrogels under quasi-static uniaxial compression is presented for a variety of compositions and test temperatures.
34619636	4	9	theme	simplicity	665:674	arg1	requirements					649:660	the requirements	645:660	the requirements of simplicity and accuracy both for compression and predicted tension	645:730	It is found that of all the tested models, the second order polynomial constitutive model fulfills the requirements of simplicity and accuracy both for compression and predicted tension.
34619636	0	10	theme	Hyperelastic	0:11	arg1	modeling					13:20	Hyperelastic modeling	0:20	Hyperelastic modeling of solid methyl cellulose hydrogel under quasi-static compression	0:86	Hyperelastic modeling of solid methyl cellulose hydrogel under quasi-static compression.
34619636	0	11	theme	solid	25:29	arg1	hydrogel					48:55	solid methyl cellulose hydrogel	25:55	solid methyl cellulose hydrogel	25:55	Hyperelastic modeling of solid methyl cellulose hydrogel under quasi-static compression.
34619636	1	12	theme	solid	114:118	arg1	hydrogels					142:150	solid methyl cellulose (MC) hydrogels	114:150	solid methyl cellulose (MC) hydrogels	114:150	Constitutive modeling of solid methyl cellulose (MC) hydrogels under quasi-static uniaxial compression is presented for a variety of compositions and test temperatures.
34619636	4	13	theme	constitutive	617:628	arg1	model					630:634	the second order polynomial constitutive model	589:634	the second order polynomial constitutive model	589:634	It is found that of all the tested models, the second order polynomial constitutive model fulfills the requirements of simplicity and accuracy both for compression and predicted tension.
34619636	3	14	theme	loading	531:537	arg1	modes					539:543	other loading modes	525:543	other loading modes	525:543	Due to the viscosity of the gel, the models were calibrated using compression tests only, with restrictions that ensure stability for other loading modes.
34619636	1	15	theme	methyl	120:125	arg1	hydrogels					142:150	solid methyl cellulose (MC) hydrogels	114:150	solid methyl cellulose (MC) hydrogels	114:150	Constitutive modeling of solid methyl cellulose (MC) hydrogels under quasi-static uniaxial compression is presented for a variety of compositions and test temperatures.
34619636	4	16	theme	polynomial	606:615	arg1	model					630:634	the second order polynomial constitutive model	589:634	the second order polynomial constitutive model	589:634	It is found that of all the tested models, the second order polynomial constitutive model fulfills the requirements of simplicity and accuracy both for compression and predicted tension.
34619636	0	17	theme	cellulose	38:46	arg1	hydrogel					48:55	solid methyl cellulose hydrogel	25:55	solid methyl cellulose hydrogel	25:55	Hyperelastic modeling of solid methyl cellulose hydrogel under quasi-static compression.
34619636	0	18	theme	methyl	31:36	arg1	hydrogel					48:55	solid methyl cellulose hydrogel	25:55	solid methyl cellulose hydrogel	25:55	Hyperelastic modeling of solid methyl cellulose hydrogel under quasi-static compression.
34619636	4	19	theme	order	600:604	arg1	model					630:634	the second order polynomial constitutive model	589:634	the second order polynomial constitutive model	589:634	It is found that of all the tested models, the second order polynomial constitutive model fulfills the requirements of simplicity and accuracy both for compression and predicted tension.
34619636	1	20	theme	compositions	222:233	arg1	temperatures					244:255	test temperatures	239:255	test temperatures	239:255	Constitutive modeling of solid methyl cellulose (MC) hydrogels under quasi-static uniaxial compression is presented for a variety of compositions and test temperatures.
34619636	1	20	theme	compositions	222:233	arg1	variety					211:217	a variety	209:217	a variety of compositions and test temperatures	209:255	Constitutive modeling of solid methyl cellulose (MC) hydrogels under quasi-static uniaxial compression is presented for a variety of compositions and test temperatures.
34619636	1	20	theme	compositions	222:233	arg1	compositions					222:233	compositions	222:233	compositions	222:233	Constitutive modeling of solid methyl cellulose (MC) hydrogels under quasi-static uniaxial compression is presented for a variety of compositions and test temperatures.
34619636	1	21	theme	cellulose	127:135	arg1	hydrogels					142:150	solid methyl cellulose (MC) hydrogels	114:150	solid methyl cellulose (MC) hydrogels	114:150	Constitutive modeling of solid methyl cellulose (MC) hydrogels under quasi-static uniaxial compression is presented for a variety of compositions and test temperatures.
34619636	0	22	theme	hydrogel	48:55	arg1	modeling					13:20	Hyperelastic modeling	0:20	Hyperelastic modeling of solid methyl cellulose hydrogel under quasi-static compression	0:86	Hyperelastic modeling of solid methyl cellulose hydrogel under quasi-static compression.
34619636	4	23	theme	models	581:586	arg1	fulfills					636:643	fulfills	636:643	fulfills	636:643	It is found that of all the tested models, the second order polynomial constitutive model fulfills the requirements of simplicity and accuracy both for compression and predicted tension.
34619636	4	24	dep	simplicity	665:674	arg1	both					689:692	both	689:692	both	689:692	It is found that of all the tested models, the second order polynomial constitutive model fulfills the requirements of simplicity and accuracy both for compression and predicted tension.
34619636	1	25	theme	MC	138:139	arg1	hydrogels					142:150	solid methyl cellulose (MC) hydrogels	114:150	solid methyl cellulose (MC) hydrogels	114:150	Constitutive modeling of solid methyl cellulose (MC) hydrogels under quasi-static uniaxial compression is presented for a variety of compositions and test temperatures.
34619636	2	26	theme	varying	286:292	arg1	complexity					294:303	varying complexity	286:303	varying complexity	286:303	Five constitutive models of varying complexity are examined, with the aim to identify the simplest accurate material representation.
34619636	4	27	theme	predicted	714:722	arg1	tension					724:730	predicted tension	714:730	predicted tension	714:730	It is found that of all the tested models, the second order polynomial constitutive model fulfills the requirements of simplicity and accuracy both for compression and predicted tension.
34619636	1	28	theme	test	239:242	arg1	temperatures					244:255	test temperatures	239:255	test temperatures	239:255	Constitutive modeling of solid methyl cellulose (MC) hydrogels under quasi-static uniaxial compression is presented for a variety of compositions and test temperatures.
34619636	3	29	theme	compression	457:467	arg1	tests					469:473	compression tests	457:473	compression tests	457:473	Due to the viscosity of the gel, the models were calibrated using compression tests only, with restrictions that ensure stability for other loading modes.
34619636	2	30	theme	constitutive	263:274	arg1	models					276:281	Five constitutive models	258:281	Five constitutive models of varying complexity	258:303	Five constitutive models of varying complexity are examined, with the aim to identify the simplest accurate material representation.
34619636	1	31	theme	hydrogels	142:150	arg1	modeling					102:109	Constitutive modeling	89:109	Constitutive modeling of solid methyl cellulose (MC) hydrogels under quasi-static uniaxial compression	89:190	Constitutive modeling of solid methyl cellulose (MC) hydrogels under quasi-static uniaxial compression is presented for a variety of compositions and test temperatures.
34619636	1	32	theme	temperatures	244:255	arg1	temperatures					244:255	test temperatures	239:255	test temperatures	239:255	Constitutive modeling of solid methyl cellulose (MC) hydrogels under quasi-static uniaxial compression is presented for a variety of compositions and test temperatures.
34619636	1	32	theme	temperatures	244:255	arg1	variety					211:217	a variety	209:217	a variety of compositions and test temperatures	209:255	Constitutive modeling of solid methyl cellulose (MC) hydrogels under quasi-static uniaxial compression is presented for a variety of compositions and test temperatures.
34619636	1	32	theme	temperatures	244:255	arg1	compositions					222:233	compositions	222:233	compositions	222:233	Constitutive modeling of solid methyl cellulose (MC) hydrogels under quasi-static uniaxial compression is presented for a variety of compositions and test temperatures.
34619636	0	33	theme	quasi-static	63:74	arg1	compression					76:86	quasi-static compression	63:86	quasi-static compression	63:86	Hyperelastic modeling of solid methyl cellulose hydrogel under quasi-static compression.
34619636	3	34	theme	other	525:529	arg1	modes					539:543	other loading modes	525:543	other loading modes	525:543	Due to the viscosity of the gel, the models were calibrated using compression tests only, with restrictions that ensure stability for other loading modes.
34619636	4	35	theme	second	593:598	arg1	model					630:634	the second order polynomial constitutive model	589:634	the second order polynomial constitutive model	589:634	It is found that of all the tested models, the second order polynomial constitutive model fulfills the requirements of simplicity and accuracy both for compression and predicted tension.
33321990	11	0	theme	narrower	1316:1323	arg1	weight					1335:1340	a narrower molecular weight	1314:1340	a narrower molecular weight distribution	1314:1353	Moreover, the yield-favoring conditions resulted in a narrower molecular weight distribution.
33321990	5	1	theme	molecular	654:662	arg1	structure					664:672	molecular structure	654:672	molecular structure	654:672	The regenerated residue of the pulp was characterized regarding its chemical composition, molecular structure, and cellulose conformation.
33321990	9	2	theme	0.15	1164:1167	arg1	%					1168:1168	%	1168:1168	%	1168:1168	Decreasing the steeping temperature by 2 °C increased xylan content in the residue with 0.13%-units over the whole span of applied alkali concentrations, while yield increased by 0.15%-units when extracting with 17 wt% aq.
33321990	1	3	theme	viscose	268:274	arg1	fibers					276:281	viscose fibers	268:281	viscose fibers	268:281	Steeping of cellulosic materials in aqueous solution of NaOH is a common pre-treatment in several industrial processes for production of cellulose-based products, including viscose fibers.
33321990	0	4	theme	Fibers	87:92	arg1	Steeping					54:61	Industrial Alkaline Steeping	34:61	Industrial Alkaline Steeping of Wood-Based Cellulose Fibers	34:92	Possibilities for Optimization of Industrial Alkaline Steeping of Wood-Based Cellulose Fibers.
33321990	2	5	contain	has	362:364	arg2	potential					370:378	the potential	366:378	the potential for process optimization regarding purity, yield, and degree of transformation to alkali cellulose	366:477	This study investigated whether the span of commonly applied process settings has the potential for process optimization regarding purity, yield, and degree of transformation to alkali cellulose.
33321990	2	5	contain	has	362:364	arg1	span					320:323	the span	316:323	the span of commonly applied process settings	316:360	This study investigated whether the span of commonly applied process settings has the potential for process optimization regarding purity, yield, and degree of transformation to alkali cellulose.
33321990	12	6	from	settings	1501:1508	arg1	high					1478:1481	high	1478:1481	high	1478:1481	The degree of transformation via alkali cellulose to cellulose II, as determined with Raman spectroscopy, was found to be high at all extraction settings applied.
33321990	12	6	from	settings	1501:1508	arg1	degree					1360:1365	The degree	1356:1365	The degree	1356:1365	The degree of transformation via alkali cellulose to cellulose II, as determined with Raman spectroscopy, was found to be high at all extraction settings applied.
33321990	5	7	theme	pulp	595:598	arg1	residue					580:586	The regenerated residue	564:586	The regenerated residue of the pulp	564:598	The regenerated residue of the pulp was characterized regarding its chemical composition, molecular structure, and cellulose conformation.
33321990	5	7	theme	pulp	595:598	arg1	pulp					595:598	the pulp	591:598	the pulp	591:598	The regenerated residue of the pulp was characterized regarding its chemical composition, molecular structure, and cellulose conformation.
33321990	1	8	theme	common	161:166	arg1	pre-treatment					168:180	a common pre-treatment	159:180	a common pre-treatment	159:180	Steeping of cellulosic materials in aqueous solution of NaOH is a common pre-treatment in several industrial processes for production of cellulose-based products, including viscose fibers.
33321990	1	8	theme	common	161:166	arg1	Steeping					95:102	Steeping	95:102	Steeping of cellulosic materials in aqueous solution of NaOH	95:154	Steeping of cellulosic materials in aqueous solution of NaOH is a common pre-treatment in several industrial processes for production of cellulose-based products, including viscose fibers.
33321990	8	9	theme	yield	867:871	arg1	purity					847:852	Both purity	842:852	Both purity of xylan and yield	842:871	Both purity of xylan and yield varied over the applied span of settings to an extent which makes case-adapted process optimization meaningful.
33321990	9	10	theme	applied	1108:1114	arg1	concentrations					1123:1136	applied alkali concentrations	1108:1136	applied alkali concentrations	1108:1136	Decreasing the steeping temperature by 2 °C increased xylan content in the residue with 0.13%-units over the whole span of applied alkali concentrations, while yield increased by 0.15%-units when extracting with 17 wt% aq.
33321990	9	11	theme	0.13	1073:1076	arg1	%					1077:1077	%	1077:1077	%	1077:1077	Decreasing the steeping temperature by 2 °C increased xylan content in the residue with 0.13%-units over the whole span of applied alkali concentrations, while yield increased by 0.15%-units when extracting with 17 wt% aq.
33321990	9	12	from	content	1045:1051	arg1	residue					1060:1066	the residue	1056:1066	the residue	1056:1066	Decreasing the steeping temperature by 2 °C increased xylan content in the residue with 0.13%-units over the whole span of applied alkali concentrations, while yield increased by 0.15%-units when extracting with 17 wt% aq.
33321990	9	13	with	content	1045:1051	arg1	-units					1078:1083	0.13%-units	1073:1083	0.13%-units	1073:1083	Decreasing the steeping temperature by 2 °C increased xylan content in the residue with 0.13%-units over the whole span of applied alkali concentrations, while yield increased by 0.15%-units when extracting with 17 wt% aq.
33321990	9	14	theme	%	1077:1077	arg1	-units					1078:1083	0.13%-units	1073:1083	0.13%-units	1073:1083	Decreasing the steeping temperature by 2 °C increased xylan content in the residue with 0.13%-units over the whole span of applied alkali concentrations, while yield increased by 0.15%-units when extracting with 17 wt% aq.
33321990	12	15	theme	transformation	1370:1383	arg1	high					1478:1481	high	1478:1481	high	1478:1481	The degree of transformation via alkali cellulose to cellulose II, as determined with Raman spectroscopy, was found to be high at all extraction settings applied.
33321990	12	15	theme	transformation	1370:1383	arg1	degree					1360:1365	The degree	1356:1365	The degree	1356:1365	The degree of transformation via alkali cellulose to cellulose II, as determined with Raman spectroscopy, was found to be high at all extraction settings applied.
33321990	3	16	theme	kraft	491:495	arg1	pulp					508:511	A hardwood kraft dissolving pulp	480:511	A hardwood kraft dissolving pulp	480:511	A hardwood kraft dissolving pulp was extracted with 17-20 wt% aq.
33321990	5	17	theme	chemical	632:639	arg1	composition					641:651	its chemical composition	628:651	its chemical composition	628:651	The regenerated residue of the pulp was characterized regarding its chemical composition, molecular structure, and cellulose conformation.
33321990	5	18	theme	regenerated	568:578	arg1	residue					580:586	The regenerated residue	564:586	The regenerated residue of the pulp	564:598	The regenerated residue of the pulp was characterized regarding its chemical composition, molecular structure, and cellulose conformation.
33321990	5	18	theme	regenerated	568:578	arg1	pulp					595:598	the pulp	591:598	the pulp	591:598	The regenerated residue of the pulp was characterized regarding its chemical composition, molecular structure, and cellulose conformation.
33321990	11	19	theme	yield-favoring	1276:1289	arg1	conditions					1291:1300	the yield-favoring conditions	1272:1300	the yield-favoring conditions	1272:1300	Moreover, the yield-favoring conditions resulted in a narrower molecular weight distribution.
33321990	8	20	theme	applied	889:895	arg1	span					897:900	the applied span	885:900	the applied span of settings	885:912	Both purity of xylan and yield varied over the applied span of settings to an extent which makes case-adapted process optimization meaningful.
33321990	3	21	theme	%	540:540	arg1	aq					542:543	17-20 wt% aq	532:543	17-20 wt% aq	532:543	A hardwood kraft dissolving pulp was extracted with 17-20 wt% aq.
33321990	11	22	theme	weight	1335:1340	arg1	distribution					1342:1353	a narrower molecular weight distribution	1314:1353	a narrower molecular weight distribution	1314:1353	Moreover, the yield-favoring conditions resulted in a narrower molecular weight distribution.
33321990	2	23	theme	applied	337:343	arg1	settings					353:360	commonly applied process settings	328:360	commonly applied process settings	328:360	This study investigated whether the span of commonly applied process settings has the potential for process optimization regarding purity, yield, and degree of transformation to alkali cellulose.
33321990	1	24	theme	several	185:191	arg1	fibers					276:281	viscose fibers	268:281	viscose fibers	268:281	Steeping of cellulosic materials in aqueous solution of NaOH is a common pre-treatment in several industrial processes for production of cellulose-based products, including viscose fibers.
33321990	1	24	theme	several	185:191	arg1	processes					204:212	several industrial processes	185:212	several industrial processes	185:212	Steeping of cellulosic materials in aqueous solution of NaOH is a common pre-treatment in several industrial processes for production of cellulose-based products, including viscose fibers.
33321990	2	25	theme	alkali	462:467	arg1	cellulose					469:477	alkali cellulose	462:477	alkali cellulose	462:477	This study investigated whether the span of commonly applied process settings has the potential for process optimization regarding purity, yield, and degree of transformation to alkali cellulose.
33321990	7	26	theme	xylan	815:819	arg1	Purity					805:810	Purity	805:810	Purity of xylan	805:819	Purity of xylan developed inversely.
33321990	6	27	theme	alkali	783:788	arg1	concentration					790:802	high alkali concentration	778:802	high alkali concentration	778:802	Yield was shown to be favored primarily by low temperature and secondly by high alkali concentration.
33321990	9	28	theme	whole	1094:1098	arg1	span					1100:1103	the whole span	1090:1103	the whole span of applied alkali concentrations	1090:1136	Decreasing the steeping temperature by 2 °C increased xylan content in the residue with 0.13%-units over the whole span of applied alkali concentrations, while yield increased by 0.15%-units when extracting with 17 wt% aq.
33321990	1	29	theme	industrial	193:202	arg1	fibers					276:281	viscose fibers	268:281	viscose fibers	268:281	Steeping of cellulosic materials in aqueous solution of NaOH is a common pre-treatment in several industrial processes for production of cellulose-based products, including viscose fibers.
33321990	1	29	theme	industrial	193:202	arg1	processes					204:212	several industrial processes	185:212	several industrial processes	185:212	Steeping of cellulosic materials in aqueous solution of NaOH is a common pre-treatment in several industrial processes for production of cellulose-based products, including viscose fibers.
33321990	4	30	from	°C	560:561	arg1	NaOH					546:549	NaOH	546:549	NaOH at 40-50 °C.	546:562	NaOH at 40-50 °C.
33321990	9	31	theme	wt	1200:1201	arg1	aq					1204:1205	17 wt% aq	1197:1205	17 wt% aq	1197:1205	Decreasing the steeping temperature by 2 °C increased xylan content in the residue with 0.13%-units over the whole span of applied alkali concentrations, while yield increased by 0.15%-units when extracting with 17 wt% aq.
33321990	6	32	theme	high	778:781	arg1	concentration					790:802	high alkali concentration	778:802	high alkali concentration	778:802	Yield was shown to be favored primarily by low temperature and secondly by high alkali concentration.
33321990	1	33	theme	cellulosic	107:116	arg1	materials					118:126	cellulosic materials	107:126	cellulosic materials	107:126	Steeping of cellulosic materials in aqueous solution of NaOH is a common pre-treatment in several industrial processes for production of cellulose-based products, including viscose fibers.
33321990	9	34	theme	%	1168:1168	arg1	-units					1169:1174	0.15%-units	1164:1174	0.15%-units	1164:1174	Decreasing the steeping temperature by 2 °C increased xylan content in the residue with 0.13%-units over the whole span of applied alkali concentrations, while yield increased by 0.15%-units when extracting with 17 wt% aq.
33321990	3	35	theme	hardwood	482:489	arg1	pulp					508:511	A hardwood kraft dissolving pulp	480:511	A hardwood kraft dissolving pulp	480:511	A hardwood kraft dissolving pulp was extracted with 17-20 wt% aq.
33321990	2	36	theme	transformation	444:457	arg1	yield					423:427	yield	423:427	yield	423:427	This study investigated whether the span of commonly applied process settings has the potential for process optimization regarding purity, yield, and degree of transformation to alkali cellulose.
33321990	2	36	theme	transformation	444:457	arg1	degree					434:439	degree	434:439	degree of transformation to alkali cellulose	434:477	This study investigated whether the span of commonly applied process settings has the potential for process optimization regarding purity, yield, and degree of transformation to alkali cellulose.
33321990	2	36	theme	transformation	444:457	arg1	purity					415:420	purity	415:420	purity	415:420	This study investigated whether the span of commonly applied process settings has the potential for process optimization regarding purity, yield, and degree of transformation to alkali cellulose.
33321990	1	37	theme	materials	118:126	arg1	pre-treatment					168:180	a common pre-treatment	159:180	a common pre-treatment	159:180	Steeping of cellulosic materials in aqueous solution of NaOH is a common pre-treatment in several industrial processes for production of cellulose-based products, including viscose fibers.
33321990	1	37	theme	materials	118:126	arg1	Steeping					95:102	Steeping	95:102	Steeping of cellulosic materials in aqueous solution of NaOH	95:154	Steeping of cellulosic materials in aqueous solution of NaOH is a common pre-treatment in several industrial processes for production of cellulose-based products, including viscose fibers.
33321990	10	38	theme	wt	1257:1258	arg1	%					1259:1259	20 wt%	1254:1259	20 wt%	1254:1259	NaOH, and by 0.20%-units when extracting with 20 wt%.
33321990	3	39	theme	wt	538:539	arg1	aq					542:543	17-20 wt% aq	532:543	17-20 wt% aq	532:543	A hardwood kraft dissolving pulp was extracted with 17-20 wt% aq.
33321990	0	40	theme	Alkaline	45:52	arg1	Steeping					54:61	Industrial Alkaline Steeping	34:61	Industrial Alkaline Steeping of Wood-Based Cellulose Fibers	34:92	Possibilities for Optimization of Industrial Alkaline Steeping of Wood-Based Cellulose Fibers.
33321990	12	41	theme	extraction	1490:1499	arg1	settings					1501:1508	all extraction settings	1486:1508	all extraction settings applied	1486:1516	The degree of transformation via alkali cellulose to cellulose II, as determined with Raman spectroscopy, was found to be high at all extraction settings applied.
33321990	8	42	theme	settings	905:912	arg1	span					897:900	the applied span	885:900	the applied span of settings	885:912	Both purity of xylan and yield varied over the applied span of settings to an extent which makes case-adapted process optimization meaningful.
33321990	0	43	theme	Industrial	34:43	arg1	Steeping					54:61	Industrial Alkaline Steeping	34:61	Industrial Alkaline Steeping of Wood-Based Cellulose Fibers	34:92	Possibilities for Optimization of Industrial Alkaline Steeping of Wood-Based Cellulose Fibers.
33321990	12	44	theme	alkali	1389:1394	arg1	cellulose					1396:1404	alkali cellulose	1389:1404	alkali cellulose to cellulose II	1389:1420	The degree of transformation via alkali cellulose to cellulose II, as determined with Raman spectroscopy, was found to be high at all extraction settings applied.
33321990	9	45	theme	alkali	1116:1121	arg1	concentrations					1123:1136	applied alkali concentrations	1108:1136	applied alkali concentrations	1108:1136	Decreasing the steeping temperature by 2 °C increased xylan content in the residue with 0.13%-units over the whole span of applied alkali concentrations, while yield increased by 0.15%-units when extracting with 17 wt% aq.
33321990	5	46	theme	cellulose	679:687	arg1	conformation					689:700	cellulose conformation	679:700	cellulose conformation	679:700	The regenerated residue of the pulp was characterized regarding its chemical composition, molecular structure, and cellulose conformation.
33321990	9	47	theme	concentrations	1123:1136	arg1	span					1100:1103	the whole span	1090:1103	the whole span of applied alkali concentrations	1090:1136	Decreasing the steeping temperature by 2 °C increased xylan content in the residue with 0.13%-units over the whole span of applied alkali concentrations, while yield increased by 0.15%-units when extracting with 17 wt% aq.
33321990	0	48	theme	Steeping	54:61	arg1	Optimization					18:29	Optimization	18:29	Optimization of Industrial Alkaline Steeping of Wood-Based Cellulose Fibers	18:92	Possibilities for Optimization of Industrial Alkaline Steeping of Wood-Based Cellulose Fibers.
33321990	3	49	theme	dissolving	497:506	arg1	pulp					508:511	A hardwood kraft dissolving pulp	480:511	A hardwood kraft dissolving pulp	480:511	A hardwood kraft dissolving pulp was extracted with 17-20 wt% aq.
33321990	1	50	theme	aqueous	131:137	arg1	solution					139:146	aqueous solution	131:146	aqueous solution	131:146	Steeping of cellulosic materials in aqueous solution of NaOH is a common pre-treatment in several industrial processes for production of cellulose-based products, including viscose fibers.
33321990	9	51	theme	%	1202:1202	arg1	aq					1204:1205	17 wt% aq	1197:1205	17 wt% aq	1197:1205	Decreasing the steeping temperature by 2 °C increased xylan content in the residue with 0.13%-units over the whole span of applied alkali concentrations, while yield increased by 0.15%-units when extracting with 17 wt% aq.
33321990	11	52	theme	molecular	1325:1333	arg1	weight					1335:1340	a narrower molecular weight	1314:1340	a narrower molecular weight distribution	1314:1353	Moreover, the yield-favoring conditions resulted in a narrower molecular weight distribution.
33321990	12	53	dep	degree	1360:1365	arg1	determined					1426:1435	determined	1426:1435	determined with Raman spectroscopy	1426:1459	The degree of transformation via alkali cellulose to cellulose II, as determined with Raman spectroscopy, was found to be high at all extraction settings applied.
33321990	2	54	theme	settings	353:360	arg1	span					320:323	the span	316:323	the span of commonly applied process settings	316:360	This study investigated whether the span of commonly applied process settings has the potential for process optimization regarding purity, yield, and degree of transformation to alkali cellulose.
33321990	8	55	theme	meaningful	973:982	arg1	process					952:958	case-adapted process	939:958	case-adapted process optimization meaningful	939:982	Both purity of xylan and yield varied over the applied span of settings to an extent which makes case-adapted process optimization meaningful.
33321990	1	56	theme	cellulose-based	232:246	arg1	products					248:255	cellulose-based products	232:255	cellulose-based products	232:255	Steeping of cellulosic materials in aqueous solution of NaOH is a common pre-treatment in several industrial processes for production of cellulose-based products, including viscose fibers.
33321990	12	57	theme	Raman	1442:1446	arg1	spectroscopy					1448:1459	Raman spectroscopy	1442:1459	Raman spectroscopy	1442:1459	The degree of transformation via alkali cellulose to cellulose II, as determined with Raman spectroscopy, was found to be high at all extraction settings applied.
33321990	6	58	theme	low	746:748	arg1	temperature					750:760	low temperature	746:760	low temperature	746:760	Yield was shown to be favored primarily by low temperature and secondly by high alkali concentration.
33321990	2	59	theme	process	345:351	arg1	settings					353:360	commonly applied process settings	328:360	commonly applied process settings	328:360	This study investigated whether the span of commonly applied process settings has the potential for process optimization regarding purity, yield, and degree of transformation to alkali cellulose.
33321990	1	60	from	Steeping	95:102	arg1	solution					139:146	aqueous solution	131:146	aqueous solution	131:146	Steeping of cellulosic materials in aqueous solution of NaOH is a common pre-treatment in several industrial processes for production of cellulose-based products, including viscose fibers.
33321990	1	61	theme	products	248:255	arg1	production					218:227	production	218:227	production of cellulose-based products	218:255	Steeping of cellulosic materials in aqueous solution of NaOH is a common pre-treatment in several industrial processes for production of cellulose-based products, including viscose fibers.
33321990	0	62	theme	Cellulose	77:85	arg1	Fibers					87:92	Wood-Based Cellulose Fibers	66:92	Wood-Based Cellulose Fibers	66:92	Possibilities for Optimization of Industrial Alkaline Steeping of Wood-Based Cellulose Fibers.
33321990	8	63	theme	case-adapted	939:950	arg1	process					952:958	case-adapted process	939:958	case-adapted process optimization meaningful	939:982	Both purity of xylan and yield varied over the applied span of settings to an extent which makes case-adapted process optimization meaningful.
33321990	1	64	theme	NaOH	151:154	arg1	pre-treatment					168:180	a common pre-treatment	159:180	a common pre-treatment	159:180	Steeping of cellulosic materials in aqueous solution of NaOH is a common pre-treatment in several industrial processes for production of cellulose-based products, including viscose fibers.
33321990	1	64	theme	NaOH	151:154	arg1	Steeping					95:102	Steeping	95:102	Steeping of cellulosic materials in aqueous solution of NaOH	95:154	Steeping of cellulosic materials in aqueous solution of NaOH is a common pre-treatment in several industrial processes for production of cellulose-based products, including viscose fibers.
33321990	9	65	theme	xylan	1039:1043	arg1	content					1045:1051	xylan content	1039:1051	xylan content in the residue with 0.13%-units	1039:1083	Decreasing the steeping temperature by 2 °C increased xylan content in the residue with 0.13%-units over the whole span of applied alkali concentrations, while yield increased by 0.15%-units when extracting with 17 wt% aq.
33321990	0	66	theme	Wood-Based	66:75	arg1	Fibers					87:92	Wood-Based Cellulose Fibers	66:92	Wood-Based Cellulose Fibers	66:92	Possibilities for Optimization of Industrial Alkaline Steeping of Wood-Based Cellulose Fibers.
33321990	9	67	theme	steeping	1000:1007	arg1	temperature					1009:1019	the steeping temperature	996:1019	the steeping temperature by 2 °C	996:1027	Decreasing the steeping temperature by 2 °C increased xylan content in the residue with 0.13%-units over the whole span of applied alkali concentrations, while yield increased by 0.15%-units when extracting with 17 wt% aq.
33321990	1	68	from	pre-treatment	168:180	arg1	fibers					276:281	viscose fibers	268:281	viscose fibers	268:281	Steeping of cellulosic materials in aqueous solution of NaOH is a common pre-treatment in several industrial processes for production of cellulose-based products, including viscose fibers.
33321990	1	68	from	pre-treatment	168:180	arg1	processes					204:212	several industrial processes	185:212	several industrial processes	185:212	Steeping of cellulosic materials in aqueous solution of NaOH is a common pre-treatment in several industrial processes for production of cellulose-based products, including viscose fibers.
33321990	2	69	theme	process	384:390	arg1	optimization					392:403	process optimization	384:403	process optimization regarding purity, yield, and degree of transformation to alkali cellulose	384:477	This study investigated whether the span of commonly applied process settings has the potential for process optimization regarding purity, yield, and degree of transformation to alkali cellulose.
33321990	8	70	theme	xylan	857:861	arg1	purity					847:852	Both purity	842:852	Both purity of xylan and yield	842:871	Both purity of xylan and yield varied over the applied span of settings to an extent which makes case-adapted process optimization meaningful.
32014480	2	0	theme	4-aminosalicylic	439:454	arg1	acid					456:459	4-aminosalicylic acid	439:459	4-aminosalicylic acid (Derivative 3)	439:474	First, the amino groups of chitosan have reacted with benzaldehyde (Derivative 1); second, hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4).
32014480	2	0	theme	4-aminosalicylic	439:454	arg1	Derivative					462:471	Derivative 3	462:473	Derivative 3	462:473	First, the amino groups of chitosan have reacted with benzaldehyde (Derivative 1); second, hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4).
32014480	8	1	attach	derived	1311:1317	arg2	groups					1304:1309	chitosan and functional groups	1280:1309	chitosan and functional groups derived from the different modifiers in addition to AgNPs within a single structure	1280:1393	Accordingly, combinations between chitosan and functional groups derived from the different modifiers in addition to AgNPs within a single structure have extraordinarily enhanced the efficiency of chitosan.
32014480	8	1	attach	derived	1311:1317	arg1	modifiers					1338:1346	the different modifiers	1324:1346	the different modifiers in addition to AgNPs within a single structure	1324:1393	Accordingly, combinations between chitosan and functional groups derived from the different modifiers in addition to AgNPs within a single structure have extraordinarily enhanced the efficiency of chitosan.
32014480	0	2	theme	biomaterials	71:82	arg1	activity					59:66	the antimicrobial activity	41:66	the antimicrobial activity of biomaterials	41:82	Designing, preparation and evaluation of the antimicrobial activity of biomaterials based on chitosan modified with silver nanoparticles.
32014480	4	3	theme	analysis	847:854	arg1	techniques					898:907	elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques	837:907	elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques	837:907	These derivatives and Derivative 4/AgNP composites were structurally identified using elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques.
32014480	6	4	theme	inhibition	1040:1049	arg1	zone					1051:1054	inhibition zone	1040:1054	inhibition zone analogous or superior	1040:1076	Some of them have inhibition zone analogous or superior than the utilized reference drugs.
32014480	4	5	theme	EDS	886:888	arg1	techniques					898:907	elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques	837:907	elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques	837:907	These derivatives and Derivative 4/AgNP composites were structurally identified using elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques.
32014480	6	6	contain	have	1035:1038	arg1	them					1030:1033	them	1030:1033	them	1030:1033	Some of them have inhibition zone analogous or superior than the utilized reference drugs.
32014480	6	6	contain	have	1035:1038	arg2	zone					1051:1054	inhibition zone	1040:1054	inhibition zone analogous or superior	1040:1076	Some of them have inhibition zone analogous or superior than the utilized reference drugs.
32014480	6	6	contain	have	1035:1038	arg1	Some					1022:1025	Some	1022:1025	Some	1022:1025	Some of them have inhibition zone analogous or superior than the utilized reference drugs.
32014480	6	7	theme	reference	1096:1104	arg1	drugs					1106:1110	the utilized reference drugs	1083:1110	the utilized reference drugs	1083:1110	Some of them have inhibition zone analogous or superior than the utilized reference drugs.
32014480	7	8	theme	composite	1173:1181	arg1	activity					1123:1130	Cytotoxic activity	1113:1130	Cytotoxic activity of Derivative 4 and Derivative 4/AgNPs-5 composite	1113:1181	Cytotoxic activity of Derivative 4 and Derivative 4/AgNPs-5 composite indicated that these materials are safe on normal human cells.
32014480	3	9	theme	impregnating	662:673	arg1	concentrations					691:704	impregnating three different concentrations	662:704	impregnating three different concentrations of silver nanoparticles inside Derivative 4	662:748	For further modification, three nano-biocomposites were synthesized via impregnating three different concentrations of silver nanoparticles inside Derivative 4.
32014480	4	10	theme	FTIR	857:860	arg1	techniques					898:907	elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques	837:907	elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques	837:907	These derivatives and Derivative 4/AgNP composites were structurally identified using elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques.
32014480	4	11	theme	TEM	894:896	arg1	techniques					898:907	elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques	837:907	elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques	837:907	These derivatives and Derivative 4/AgNP composites were structurally identified using elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques.
32014480	6	12	theme	utilized	1087:1094	arg1	drugs					1106:1110	the utilized reference drugs	1083:1110	the utilized reference drugs	1083:1110	Some of them have inhibition zone analogous or superior than the utilized reference drugs.
32014480	8	13	dep	AgNPs	1363:1367	arg1	addition					1351:1358	addition	1351:1358	addition	1351:1358	Accordingly, combinations between chitosan and functional groups derived from the different modifiers in addition to AgNPs within a single structure have extraordinarily enhanced the efficiency of chitosan.
32014480	7	14	theme	Derivative	1152:1161	arg1	composite					1173:1181	Derivative 4 and Derivative 4/AgNPs-5 composite	1135:1181	composite	1173:1181	Cytotoxic activity of Derivative 4 and Derivative 4/AgNPs-5 composite indicated that these materials are safe on normal human cells.
32014480	2	15	theme	amino	561:565	arg1	Derivative					575:584	Derivative 4	575:586	Derivative 4	575:586	First, the amino groups of chitosan have reacted with benzaldehyde (Derivative 1); second, hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4).
32014480	2	15	theme	amino	561:565	arg1	groups					567:572	the amino groups	557:572	the amino groups (Derivative 4)	557:587	First, the amino groups of chitosan have reacted with benzaldehyde (Derivative 1); second, hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4).
32014480	8	16	theme	different	1328:1336	arg1	modifiers					1338:1346	the different modifiers	1324:1346	the different modifiers in addition to AgNPs within a single structure	1324:1393	Accordingly, combinations between chitosan and functional groups derived from the different modifiers in addition to AgNPs within a single structure have extraordinarily enhanced the efficiency of chitosan.
32014480	2	17	theme	amino	213:217	arg1	chitosan					229:236	chitosan	229:236	chitosan	229:236	First, the amino groups of chitosan have reacted with benzaldehyde (Derivative 1); second, hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4).
32014480	2	17	theme	amino	213:217	arg1	groups					219:224	the amino groups	209:224	the amino groups of chitosan	209:236	First, the amino groups of chitosan have reacted with benzaldehyde (Derivative 1); second, hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4).
32014480	1	18	theme	four-step	181:189	arg1	procedure					191:199	a four-step procedure	179:199	a four-step procedure	179:199	Chitosan was chemically modified through a four-step procedure.
32014480	5	19	theme	superior	966:973	arg1	activities					989:998	superior antimicrobial activities	966:998	superior antimicrobial activities	966:998	These derivatives and Derivative 4/AgNP composites have superior antimicrobial activities than virgin chitosan.
32014480	4	20	theme	elemental	837:845	arg1	analysis					847:854	elemental analysis	837:854	elemental analysis	837:854	These derivatives and Derivative 4/AgNP composites were structurally identified using elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques.
32014480	9	21	theme	proper	1532:1537	arg1	frameworks					1502:1511	promising frameworks	1492:1511	promising frameworks which are taken as proper competitors for antimicrobial materials in biomedical fields	1492:1598	It might be deemed as a path to attain promising frameworks which are taken as proper competitors for antimicrobial materials in biomedical fields.
32014480	9	21	theme	proper	1532:1537	arg1	competitors					1539:1549	proper competitors	1532:1549	proper competitors for antimicrobial materials in biomedical fields	1532:1598	It might be deemed as a path to attain promising frameworks which are taken as proper competitors for antimicrobial materials in biomedical fields.
32014480	2	22	theme	Derivative	514:523	arg1	moieties					502:509	benzaldehyde moieties	489:509	benzaldehyde moieties of Derivative 3	489:525	First, the amino groups of chitosan have reacted with benzaldehyde (Derivative 1); second, hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4).
32014480	9	23	theme	biomedical	1582:1591	arg1	fields					1593:1598	biomedical fields	1582:1598	biomedical fields	1582:1598	It might be deemed as a path to attain promising frameworks which are taken as proper competitors for antimicrobial materials in biomedical fields.
32014480	2	24	theme	hydroxyl	293:300	arg1	groups					302:307	hydroxyl groups	293:307	hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4)	293:587	First, the amino groups of chitosan have reacted with benzaldehyde (Derivative 1); second, hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4).
32014480	2	25	dep	benzaldehyde	256:267	arg1	groups					302:307	hydroxyl groups	293:307	hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4)	293:587	First, the amino groups of chitosan have reacted with benzaldehyde (Derivative 1); second, hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4).
32014480	2	26	theme	epoxy	392:396	arg1	groups					398:403	the epoxy groups	388:403	the epoxy groups of Derivative 2	388:419	First, the amino groups of chitosan have reacted with benzaldehyde (Derivative 1); second, hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4).
32014480	2	26	theme	epoxy	392:396	arg1	Derivative					408:417	Derivative 2	408:419	Derivative 2	408:419	First, the amino groups of chitosan have reacted with benzaldehyde (Derivative 1); second, hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4).
32014480	3	27	theme	silver	709:714	arg1	nanoparticles					716:728	silver nanoparticles	709:728	silver nanoparticles inside Derivative 4	709:748	For further modification, three nano-biocomposites were synthesized via impregnating three different concentrations of silver nanoparticles inside Derivative 4.
32014480	2	28	theme	benzaldehyde	489:500	arg1	moieties					502:509	benzaldehyde moieties	489:509	benzaldehyde moieties of Derivative 3	489:525	First, the amino groups of chitosan have reacted with benzaldehyde (Derivative 1); second, hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4).
32014480	9	29	theme	antimicrobial	1555:1567	arg1	materials					1569:1577	antimicrobial materials	1555:1577	antimicrobial materials in biomedical fields	1555:1598	It might be deemed as a path to attain promising frameworks which are taken as proper competitors for antimicrobial materials in biomedical fields.
32014480	3	30	theme	different	681:689	arg1	concentrations					691:704	impregnating three different concentrations	662:704	impregnating three different concentrations of silver nanoparticles inside Derivative 4	662:748	For further modification, three nano-biocomposites were synthesized via impregnating three different concentrations of silver nanoparticles inside Derivative 4.
32014480	0	31	theme	silver	116:121	arg1	nanoparticles					123:135	silver nanoparticles	116:135	silver nanoparticles	116:135	Designing, preparation and evaluation of the antimicrobial activity of biomaterials based on chitosan modified with silver nanoparticles.
32014480	2	32	theme	second	285:290	arg1	groups					302:307	hydroxyl groups	293:307	hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4)	293:587	First, the amino groups of chitosan have reacted with benzaldehyde (Derivative 1); second, hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4).
32014480	8	33	theme	functional	1293:1302	arg1	groups					1304:1309	chitosan and functional groups	1280:1309	chitosan and functional groups derived from the different modifiers in addition to AgNPs within a single structure	1280:1393	Accordingly, combinations between chitosan and functional groups derived from the different modifiers in addition to AgNPs within a single structure have extraordinarily enhanced the efficiency of chitosan.
32014480	4	34	theme	SEM	881:883	arg1	techniques					898:907	elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques	837:907	elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques	837:907	These derivatives and Derivative 4/AgNP composites were structurally identified using elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques.
32014480	4	35	theme	4/AgNP	784:789	arg1	composites					791:800	Derivative 4/AgNP composites	773:800	Derivative 4/AgNP composites	773:800	These derivatives and Derivative 4/AgNP composites were structurally identified using elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques.
32014480	7	36	theme	Cytotoxic	1113:1121	arg1	activity					1123:1130	Cytotoxic activity	1113:1130	Cytotoxic activity of Derivative 4 and Derivative 4/AgNPs-5 composite	1113:1181	Cytotoxic activity of Derivative 4 and Derivative 4/AgNPs-5 composite indicated that these materials are safe on normal human cells.
32014480	2	37	theme	chitosan	229:236	arg1	chitosan					229:236	chitosan	229:236	chitosan	229:236	First, the amino groups of chitosan have reacted with benzaldehyde (Derivative 1); second, hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4).
32014480	2	37	theme	chitosan	229:236	arg1	groups					219:224	the amino groups	209:224	the amino groups of chitosan	209:236	First, the amino groups of chitosan have reacted with benzaldehyde (Derivative 1); second, hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4).
32014480	0	38	theme	antimicrobial	45:57	arg1	activity					59:66	the antimicrobial activity	41:66	the antimicrobial activity of biomaterials	41:82	Designing, preparation and evaluation of the antimicrobial activity of biomaterials based on chitosan modified with silver nanoparticles.
32014480	7	39	theme	4/AgNPs-5	1163:1171	arg1	composite					1173:1181	Derivative 4 and Derivative 4/AgNPs-5 composite	1135:1181	composite	1173:1181	Cytotoxic activity of Derivative 4 and Derivative 4/AgNPs-5 composite indicated that these materials are safe on normal human cells.
32014480	9	40	theme	promising	1492:1500	arg1	frameworks					1502:1511	promising frameworks	1492:1511	promising frameworks which are taken as proper competitors for antimicrobial materials in biomedical fields	1492:1598	It might be deemed as a path to attain promising frameworks which are taken as proper competitors for antimicrobial materials in biomedical fields.
32014480	9	40	theme	promising	1492:1500	arg1	competitors					1539:1549	proper competitors	1532:1549	proper competitors for antimicrobial materials in biomedical fields	1532:1598	It might be deemed as a path to attain promising frameworks which are taken as proper competitors for antimicrobial materials in biomedical fields.
32014480	8	41	theme	chitosan	1443:1450	arg1	efficiency					1429:1438	the efficiency	1425:1438	the efficiency of chitosan	1425:1450	Accordingly, combinations between chitosan and functional groups derived from the different modifiers in addition to AgNPs within a single structure have extraordinarily enhanced the efficiency of chitosan.
32014480	5	42	theme	antimicrobial	975:987	arg1	activities					989:998	superior antimicrobial activities	966:998	superior antimicrobial activities	966:998	These derivatives and Derivative 4/AgNP composites have superior antimicrobial activities than virgin chitosan.
32014480	2	43	theme	Derivative	318:327	arg1	C6					312:313	C6	312:313	C6 of Derivative 1	312:329	First, the amino groups of chitosan have reacted with benzaldehyde (Derivative 1); second, hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4).
32014480	2	44	theme	Derivative	408:417	arg1	groups					398:403	the epoxy groups	388:403	the epoxy groups of Derivative 2	388:419	First, the amino groups of chitosan have reacted with benzaldehyde (Derivative 1); second, hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4).
32014480	2	44	theme	Derivative	408:417	arg1	Derivative					408:417	Derivative 2	408:419	Derivative 2	408:419	First, the amino groups of chitosan have reacted with benzaldehyde (Derivative 1); second, hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic acid (Derivative 3); and fourth, benzaldehyde moieties of Derivative 3 have been removed to retrieve the amino groups (Derivative 4).
32014480	3	45	theme	nanoparticles	716:728	arg1	concentrations					691:704	impregnating three different concentrations	662:704	impregnating three different concentrations of silver nanoparticles inside Derivative 4	662:748	For further modification, three nano-biocomposites were synthesized via impregnating three different concentrations of silver nanoparticles inside Derivative 4.
32014480	4	46	theme	XPS	863:865	arg1	techniques					898:907	elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques	837:907	elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques	837:907	These derivatives and Derivative 4/AgNP composites were structurally identified using elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques.
32014480	3	47	theme	further	594:600	arg1	modification					602:613	further modification	594:613	further modification	594:613	For further modification, three nano-biocomposites were synthesized via impregnating three different concentrations of silver nanoparticles inside Derivative 4.
32014480	8	48	theme	single	1378:1383	arg1	structure					1385:1393	a single structure	1376:1393	a single structure	1376:1393	Accordingly, combinations between chitosan and functional groups derived from the different modifiers in addition to AgNPs within a single structure have extraordinarily enhanced the efficiency of chitosan.
32014480	8	49	from	modifiers	1338:1346	arg1	AgNPs					1363:1367	AgNPs	1363:1367	AgNPs within a single structure	1363:1393	Accordingly, combinations between chitosan and functional groups derived from the different modifiers in addition to AgNPs within a single structure have extraordinarily enhanced the efficiency of chitosan.
32014480	7	50	theme	normal	1226:1231	arg1	cells					1239:1243	normal human cells	1226:1243	normal human cells	1226:1243	Cytotoxic activity of Derivative 4 and Derivative 4/AgNPs-5 composite indicated that these materials are safe on normal human cells.
32014480	4	51	theme	NMR	871:873	arg1	techniques					898:907	elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques	837:907	elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques	837:907	These derivatives and Derivative 4/AgNP composites were structurally identified using elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques.
32014480	5	52	theme	virgin	1005:1010	arg1	chitosan					1012:1019	virgin chitosan	1005:1019	virgin chitosan	1005:1019	These derivatives and Derivative 4/AgNP composites have superior antimicrobial activities than virgin chitosan.
32014480	4	53	theme	Derivative	773:782	arg1	composites					791:800	Derivative 4/AgNP composites	773:800	Derivative 4/AgNP composites	773:800	These derivatives and Derivative 4/AgNP composites were structurally identified using elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques.
32014480	7	54	theme	human	1233:1237	arg1	cells					1239:1243	normal human cells	1226:1243	normal human cells	1226:1243	Cytotoxic activity of Derivative 4 and Derivative 4/AgNPs-5 composite indicated that these materials are safe on normal human cells.
32014480	9	55	from	materials	1569:1577	arg1	fields					1593:1598	biomedical fields	1582:1598	biomedical fields	1582:1598	It might be deemed as a path to attain promising frameworks which are taken as proper competitors for antimicrobial materials in biomedical fields.
32014480	4	56	theme	1H	868:869	arg1	NMR					871:873	1H NMR	868:873	1H NMR	868:873	These derivatives and Derivative 4/AgNP composites were structurally identified using elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques.
32014480	5	57	theme	Derivative	932:941	arg1	composites					950:959	Derivative 4/AgNP composites	932:959	Derivative 4/AgNP composites	932:959	These derivatives and Derivative 4/AgNP composites have superior antimicrobial activities than virgin chitosan.
32014480	6	58	theme	superior	1069:1076	arg1	zone					1051:1054	inhibition zone	1040:1054	inhibition zone analogous or superior	1040:1076	Some of them have inhibition zone analogous or superior than the utilized reference drugs.
32014480	8	59	theme	chitosan	1280:1287	arg1	groups					1304:1309	chitosan and functional groups	1280:1309	chitosan and functional groups derived from the different modifiers in addition to AgNPs within a single structure	1280:1393	Accordingly, combinations between chitosan and functional groups derived from the different modifiers in addition to AgNPs within a single structure have extraordinarily enhanced the efficiency of chitosan.
32014480	4	60	theme	XRD	876:878	arg1	techniques					898:907	elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques	837:907	elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques	837:907	These derivatives and Derivative 4/AgNP composites were structurally identified using elemental analysis, FTIR, XPS, 1H NMR, XRD, SEM, EDS and TEM techniques.
32014480	5	61	theme	4/AgNP	943:948	arg1	composites					950:959	Derivative 4/AgNP composites	932:959	Derivative 4/AgNP composites	932:959	These derivatives and Derivative 4/AgNP composites have superior antimicrobial activities than virgin chitosan.
32014480	7	62	theme	Derivative	1135:1144	arg1	activity					1123:1130	Cytotoxic activity	1113:1130	Cytotoxic activity of Derivative 4 and Derivative 4/AgNPs-5 composite	1113:1181	Cytotoxic activity of Derivative 4 and Derivative 4/AgNPs-5 composite indicated that these materials are safe on normal human cells.
32014480	5	63	contain	have	961:964	arg2	activities					989:998	superior antimicrobial activities	966:998	superior antimicrobial activities	966:998	These derivatives and Derivative 4/AgNP composites have superior antimicrobial activities than virgin chitosan.
32014480	5	63	contain	have	961:964	arg1	composites					950:959	Derivative 4/AgNP composites	932:959	Derivative 4/AgNP composites	932:959	These derivatives and Derivative 4/AgNP composites have superior antimicrobial activities than virgin chitosan.
32014480	5	63	contain	have	961:964	arg1	derivatives					916:926	These derivatives	910:926	These derivatives	910:926	These derivatives and Derivative 4/AgNP composites have superior antimicrobial activities than virgin chitosan.
32014480	6	64	theme	analogous	1056:1064	arg1	zone					1051:1054	inhibition zone	1040:1054	inhibition zone analogous or superior	1040:1076	Some of them have inhibition zone analogous or superior than the utilized reference drugs.
32464206	1	0	theme	tetrachloride	263:275	arg1	mice					292:295	carbon tetrachloride (CCl4) induced mice	256:295	carbon tetrachloride (CCl4) induced mice	256:295	The study was to investigate the anti-inflammatory effect of Cyclocarya paliurus polysaccharides (CP) on carbon tetrachloride (CCl4) induced mice and explore its underlying mechanism.
32464206	5	1	theme	16S	684:686	arg1	rDNA					688:691	16S rDNA	684:691	16S rDNA	684:691	Furthermore, 16S rDNA showed that CP increased the Firmicutes/Bacteroidetes ratio and the abundance of probiotics (Unclassified Clostridiales) as well as decreased abundances of pathogenic bacteria (Proteobacteria, Helicobacter and Aerococcus) compared to model group.
32464206	2	2	with	pretreatment	397:408	arg1	CP					415:416	CP	415:416	CP	415:416	Based on a mouse model of CCl4 induced liver inflammation and pretreatment with CP.
32464206	0	3	theme	microbiota	106:115	arg1	regulation					88:97	beneficial regulation	77:97	beneficial regulation of gut microbiota and TLR4/MAPK signaling pathways	77:148	Cyclocarya paliurus polysaccharide alleviates liver inflammation in mice via beneficial regulation of gut microbiota and TLR4/MAPK signaling pathways.
32464206	2	4	from	Based	335:339	arg1	model					352:356	a mouse model	344:356	a mouse model of CCl4	344:364	Based on a mouse model of CCl4 induced liver inflammation and pretreatment with CP.
32464206	6	5	theme	SCFAs	977:981	arg1	levels					983:988	the SCFAs levels	973:988	the SCFAs levels in CCl4 induced mice	973:1009	Moreover, CP treatment increased the SCFAs levels in CCl4 induced mice.
32464206	0	6	theme	gut	102:104	arg1	microbiota					106:115	gut microbiota	102:115	gut microbiota	102:115	Cyclocarya paliurus polysaccharide alleviates liver inflammation in mice via beneficial regulation of gut microbiota and TLR4/MAPK signaling pathways.
32464206	0	7	theme	TLR4/MAPK	121:129	arg1	pathways					141:148	TLR4/MAPK signaling pathways	121:148	TLR4/MAPK signaling pathways	121:148	Cyclocarya paliurus polysaccharide alleviates liver inflammation in mice via beneficial regulation of gut microbiota and TLR4/MAPK signaling pathways.
32464206	3	8	theme	cytokines	500:508	arg1	levels					564:569	the proteins expression levels	540:569	the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS	466:587	Results showed that CP significantly decreased the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS in the liver.
32464206	3	8	theme	cytokines	500:508	arg1	levels					470:475	the levels	466:475	the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS	466:587	Results showed that CP significantly decreased the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS in the liver.
32464206	5	9	theme	Unclassified	786:797	arg1	probiotics					774:783	probiotics	774:783	probiotics (Unclassified Clostridiales)	774:812	Furthermore, 16S rDNA showed that CP increased the Firmicutes/Bacteroidetes ratio and the abundance of probiotics (Unclassified Clostridiales) as well as decreased abundances of pathogenic bacteria (Proteobacteria, Helicobacter and Aerococcus) compared to model group.
32464206	5	9	theme	Unclassified	786:797	arg1	Clostridiales					799:811	Unclassified Clostridiales	786:811	Unclassified Clostridiales	786:811	Furthermore, 16S rDNA showed that CP increased the Firmicutes/Bacteroidetes ratio and the abundance of probiotics (Unclassified Clostridiales) as well as decreased abundances of pathogenic bacteria (Proteobacteria, Helicobacter and Aerococcus) compared to model group.
32464206	3	10	dep	cytokines	500:508	arg1	IL-1β					522:526	IL-1β	522:526	IL-1β	522:526	Results showed that CP significantly decreased the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS in the liver.
32464206	3	10	dep	cytokines	500:508	arg1	TNF-α					515:519	TNF-α	515:519	TNF-α	515:519	Results showed that CP significantly decreased the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS in the liver.
32464206	3	10	dep	cytokines	500:508	arg1	NO					511:512	NO	511:512	NO	511:512	Results showed that CP significantly decreased the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS in the liver.
32464206	0	11	from	inflammation	52:63	arg1	mice					68:71	mice	68:71	mice	68:71	Cyclocarya paliurus polysaccharide alleviates liver inflammation in mice via beneficial regulation of gut microbiota and TLR4/MAPK signaling pathways.
32464206	7	12	theme	liver	1083:1087	arg1	inflammation					1089:1100	the liver inflammation	1079:1100	the liver inflammation in mice caused by CCl4	1079:1123	These findings suggested that CP act as prebiotics in ameliorating the liver inflammation in mice caused by CCl4 through regulating the gut microbiota composition and increasing the concentration of SCFAs.
32464206	5	13	theme	model	927:931	arg1	group					933:937	model group	927:937	model group	927:937	Furthermore, 16S rDNA showed that CP increased the Firmicutes/Bacteroidetes ratio and the abundance of probiotics (Unclassified Clostridiales) as well as decreased abundances of pathogenic bacteria (Proteobacteria, Helicobacter and Aerococcus) compared to model group.
32464206	1	14	theme	anti-inflammatory	184:200	arg1	effect					202:207	the anti-inflammatory effect	180:207	the anti-inflammatory effect of Cyclocarya paliurus polysaccharides (CP) on carbon tetrachloride (CCl4) induced mice	180:295	The study was to investigate the anti-inflammatory effect of Cyclocarya paliurus polysaccharides (CP) on carbon tetrachloride (CCl4) induced mice and explore its underlying mechanism.
32464206	0	15	theme	paliurus	11:18	arg1	polysaccharide					20:33	Cyclocarya paliurus polysaccharide	0:33	Cyclocarya paliurus polysaccharide	0:33	Cyclocarya paliurus polysaccharide alleviates liver inflammation in mice via beneficial regulation of gut microbiota and TLR4/MAPK signaling pathways.
32464206	7	16	theme	SCFAs	1211:1215	arg1	concentration					1194:1206	the concentration	1190:1206	the concentration of SCFAs	1190:1215	These findings suggested that CP act as prebiotics in ameliorating the liver inflammation in mice caused by CCl4 through regulating the gut microbiota composition and increasing the concentration of SCFAs.
32464206	5	17	theme	Firmicutes/Bacteroidetes	722:745	arg1	ratio					747:751	the Firmicutes/Bacteroidetes ratio	718:751	the Firmicutes/Bacteroidetes ratio	718:751	Furthermore, 16S rDNA showed that CP increased the Firmicutes/Bacteroidetes ratio and the abundance of probiotics (Unclassified Clostridiales) as well as decreased abundances of pathogenic bacteria (Proteobacteria, Helicobacter and Aerococcus) compared to model group.
32464206	3	18	theme	proteins	544:551	arg1	levels					564:569	the proteins expression levels	540:569	the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS	466:587	Results showed that CP significantly decreased the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS in the liver.
32464206	7	19	theme	microbiota	1152:1161	arg1	composition					1163:1173	the gut microbiota composition	1144:1173	the gut microbiota composition	1144:1173	These findings suggested that CP act as prebiotics in ameliorating the liver inflammation in mice caused by CCl4 through regulating the gut microbiota composition and increasing the concentration of SCFAs.
32464206	5	20	theme	bacteria	860:867	arg1	abundances					835:844	abundances	835:844	abundances of pathogenic bacteria	835:867	Furthermore, 16S rDNA showed that CP increased the Firmicutes/Bacteroidetes ratio and the abundance of probiotics (Unclassified Clostridiales) as well as decreased abundances of pathogenic bacteria (Proteobacteria, Helicobacter and Aerococcus) compared to model group.
32464206	0	21	theme	Cyclocarya	0:9	arg1	polysaccharide					20:33	Cyclocarya paliurus polysaccharide	0:33	Cyclocarya paliurus polysaccharide	0:33	Cyclocarya paliurus polysaccharide alleviates liver inflammation in mice via beneficial regulation of gut microbiota and TLR4/MAPK signaling pathways.
32464206	1	22	theme	induced	284:290	arg1	mice					292:295	carbon tetrachloride (CCl4) induced mice	256:295	carbon tetrachloride (CCl4) induced mice	256:295	The study was to investigate the anti-inflammatory effect of Cyclocarya paliurus polysaccharides (CP) on carbon tetrachloride (CCl4) induced mice and explore its underlying mechanism.
32464206	6	23	from	levels	983:988	arg1	mice					1006:1009	CCl4 induced mice	993:1009	CCl4 induced mice	993:1009	Moreover, CP treatment increased the SCFAs levels in CCl4 induced mice.
32464206	3	24	theme	expression	553:562	arg1	levels					564:569	the proteins expression levels	540:569	the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS	466:587	Results showed that CP significantly decreased the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS in the liver.
32464206	2	25	theme	liver	374:378	arg1	inflammation					380:391	liver inflammation	374:391	liver inflammation	374:391	Based on a mouse model of CCl4 induced liver inflammation and pretreatment with CP.
32464206	0	26	theme	pathways	141:148	arg1	regulation					88:97	beneficial regulation	77:97	beneficial regulation of gut microbiota and TLR4/MAPK signaling pathways	77:148	Cyclocarya paliurus polysaccharide alleviates liver inflammation in mice via beneficial regulation of gut microbiota and TLR4/MAPK signaling pathways.
32464206	1	27	theme	Cyclocarya	212:221	arg1	CP					249:250	CP	249:250	CP	249:250	The study was to investigate the anti-inflammatory effect of Cyclocarya paliurus polysaccharides (CP) on carbon tetrachloride (CCl4) induced mice and explore its underlying mechanism.
32464206	1	27	theme	Cyclocarya	212:221	arg1	polysaccharides					232:246	Cyclocarya paliurus polysaccharides	212:246	Cyclocarya paliurus polysaccharides (CP)	212:251	The study was to investigate the anti-inflammatory effect of Cyclocarya paliurus polysaccharides (CP) on carbon tetrachloride (CCl4) induced mice and explore its underlying mechanism.
32464206	7	28	theme	gut	1148:1150	arg1	composition					1163:1173	the gut microbiota composition	1144:1173	the gut microbiota composition	1144:1173	These findings suggested that CP act as prebiotics in ameliorating the liver inflammation in mice caused by CCl4 through regulating the gut microbiota composition and increasing the concentration of SCFAs.
32464206	6	29	theme	CP	950:951	arg1	treatment					953:961	CP treatment	950:961	CP treatment	950:961	Moreover, CP treatment increased the SCFAs levels in CCl4 induced mice.
32464206	0	30	theme	signaling	131:139	arg1	pathways					141:148	TLR4/MAPK signaling pathways	121:148	TLR4/MAPK signaling pathways	121:148	Cyclocarya paliurus polysaccharide alleviates liver inflammation in mice via beneficial regulation of gut microbiota and TLR4/MAPK signaling pathways.
32464206	1	31	theme	paliurus	223:230	arg1	CP					249:250	CP	249:250	CP	249:250	The study was to investigate the anti-inflammatory effect of Cyclocarya paliurus polysaccharides (CP) on carbon tetrachloride (CCl4) induced mice and explore its underlying mechanism.
32464206	1	31	theme	paliurus	223:230	arg1	polysaccharides					232:246	Cyclocarya paliurus polysaccharides	212:246	Cyclocarya paliurus polysaccharides (CP)	212:251	The study was to investigate the anti-inflammatory effect of Cyclocarya paliurus polysaccharides (CP) on carbon tetrachloride (CCl4) induced mice and explore its underlying mechanism.
32464206	4	32	theme	MAPK	612:615	arg1	pathways					617:624	MAPK pathways	612:624	MAPK pathways	612:624	TLR4 and MAPK pathways were down-regulated after CP administration.
32464206	3	33	theme	COX-2	574:578	arg1	levels					564:569	the proteins expression levels	540:569	the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS	466:587	Results showed that CP significantly decreased the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS in the liver.
32464206	3	33	theme	COX-2	574:578	arg1	levels					470:475	the levels	466:475	the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS	466:587	Results showed that CP significantly decreased the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS in the liver.
32464206	1	34	theme	polysaccharides	232:246	arg1	effect					202:207	the anti-inflammatory effect	180:207	the anti-inflammatory effect of Cyclocarya paliurus polysaccharides (CP) on carbon tetrachloride (CCl4) induced mice	180:295	The study was to investigate the anti-inflammatory effect of Cyclocarya paliurus polysaccharides (CP) on carbon tetrachloride (CCl4) induced mice and explore its underlying mechanism.
32464206	0	35	theme	liver	46:50	arg1	inflammation					52:63	liver inflammation	46:63	liver inflammation in mice	46:71	Cyclocarya paliurus polysaccharide alleviates liver inflammation in mice via beneficial regulation of gut microbiota and TLR4/MAPK signaling pathways.
32464206	7	36	from	inflammation	1089:1100	arg1	mice					1105:1108	mice	1105:1108	mice caused by CCl4	1105:1123	These findings suggested that CP act as prebiotics in ameliorating the liver inflammation in mice caused by CCl4 through regulating the gut microbiota composition and increasing the concentration of SCFAs.
32464206	5	37	theme	probiotics	774:783	arg1	ratio					747:751	the Firmicutes/Bacteroidetes ratio	718:751	the Firmicutes/Bacteroidetes ratio	718:751	Furthermore, 16S rDNA showed that CP increased the Firmicutes/Bacteroidetes ratio and the abundance of probiotics (Unclassified Clostridiales) as well as decreased abundances of pathogenic bacteria (Proteobacteria, Helicobacter and Aerococcus) compared to model group.
32464206	5	37	theme	probiotics	774:783	arg1	abundance					761:769	the abundance	757:769	the abundance of probiotics (Unclassified Clostridiales)	757:812	Furthermore, 16S rDNA showed that CP increased the Firmicutes/Bacteroidetes ratio and the abundance of probiotics (Unclassified Clostridiales) as well as decreased abundances of pathogenic bacteria (Proteobacteria, Helicobacter and Aerococcus) compared to model group.
32464206	1	38	theme	underlying	313:322	arg1	mechanism					324:332	its underlying mechanism	309:332	its underlying mechanism	309:332	The study was to investigate the anti-inflammatory effect of Cyclocarya paliurus polysaccharides (CP) on carbon tetrachloride (CCl4) induced mice and explore its underlying mechanism.
32464206	2	39	theme	mouse	346:350	arg1	model					352:356	a mouse model	344:356	a mouse model of CCl4	344:364	Based on a mouse model of CCl4 induced liver inflammation and pretreatment with CP.
32464206	5	40	dep	decreased	825:833	arg1	Helicobacter					886:897	Helicobacter	886:897	Helicobacter	886:897	Furthermore, 16S rDNA showed that CP increased the Firmicutes/Bacteroidetes ratio and the abundance of probiotics (Unclassified Clostridiales) as well as decreased abundances of pathogenic bacteria (Proteobacteria, Helicobacter and Aerococcus) compared to model group.
32464206	5	40	dep	decreased	825:833	arg1	Proteobacteria					870:883	Proteobacteria	870:883	Proteobacteria	870:883	Furthermore, 16S rDNA showed that CP increased the Firmicutes/Bacteroidetes ratio and the abundance of probiotics (Unclassified Clostridiales) as well as decreased abundances of pathogenic bacteria (Proteobacteria, Helicobacter and Aerococcus) compared to model group.
32464206	5	40	dep	decreased	825:833	arg1	Aerococcus					903:912	Aerococcus	903:912	Aerococcus	903:912	Furthermore, 16S rDNA showed that CP increased the Firmicutes/Bacteroidetes ratio and the abundance of probiotics (Unclassified Clostridiales) as well as decreased abundances of pathogenic bacteria (Proteobacteria, Helicobacter and Aerococcus) compared to model group.
32464206	4	41	theme	CP	652:653	arg1	administration					655:668	CP administration	652:668	CP administration	652:668	TLR4 and MAPK pathways were down-regulated after CP administration.
32464206	1	42	from	effect	202:207	arg1	mice					292:295	carbon tetrachloride (CCl4) induced mice	256:295	carbon tetrachloride (CCl4) induced mice	256:295	The study was to investigate the anti-inflammatory effect of Cyclocarya paliurus polysaccharides (CP) on carbon tetrachloride (CCl4) induced mice and explore its underlying mechanism.
32464206	3	43	theme	proinflammatory	484:498	arg1	cytokines					500:508	the proinflammatory cytokines	480:508	the proinflammatory cytokines (NO, TNF-α, IL-1β)	480:527	Results showed that CP significantly decreased the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS in the liver.
32464206	0	44	theme	beneficial	77:86	arg1	regulation					88:97	beneficial regulation	77:97	beneficial regulation of gut microbiota and TLR4/MAPK signaling pathways	77:148	Cyclocarya paliurus polysaccharide alleviates liver inflammation in mice via beneficial regulation of gut microbiota and TLR4/MAPK signaling pathways.
32464206	3	45	theme	iNOS	584:587	arg1	levels					564:569	the proteins expression levels	540:569	the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS	466:587	Results showed that CP significantly decreased the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS in the liver.
32464206	3	45	theme	iNOS	584:587	arg1	levels					470:475	the levels	466:475	the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS	466:587	Results showed that CP significantly decreased the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS in the liver.
32464206	5	46	theme	pathogenic	849:858	arg1	bacteria					860:867	pathogenic bacteria	849:867	pathogenic bacteria	849:867	Furthermore, 16S rDNA showed that CP increased the Firmicutes/Bacteroidetes ratio and the abundance of probiotics (Unclassified Clostridiales) as well as decreased abundances of pathogenic bacteria (Proteobacteria, Helicobacter and Aerococcus) compared to model group.
32464206	6	47	theme	induced	998:1004	arg1	mice					1006:1009	CCl4 induced mice	993:1009	CCl4 induced mice	993:1009	Moreover, CP treatment increased the SCFAs levels in CCl4 induced mice.
32464206	2	48	with	inflammation	380:391	arg1	CP					415:416	CP	415:416	CP	415:416	Based on a mouse model of CCl4 induced liver inflammation and pretreatment with CP.
32464206	6	49	theme	CCl4	993:996	arg1	mice					1006:1009	CCl4 induced mice	993:1009	CCl4 induced mice	993:1009	Moreover, CP treatment increased the SCFAs levels in CCl4 induced mice.
32464206	2	50	theme	CCl4	361:364	arg1	model					352:356	a mouse model	344:356	a mouse model of CCl4	344:364	Based on a mouse model of CCl4 induced liver inflammation and pretreatment with CP.
32464206	1	51	theme	carbon	256:261	arg1	CCl4					278:281	CCl4	278:281	CCl4	278:281	The study was to investigate the anti-inflammatory effect of Cyclocarya paliurus polysaccharides (CP) on carbon tetrachloride (CCl4) induced mice and explore its underlying mechanism.
32464206	1	51	theme	carbon	256:261	arg1	tetrachloride					263:275	carbon tetrachloride	256:275	carbon tetrachloride (CCl4) induced mice	256:295	The study was to investigate the anti-inflammatory effect of Cyclocarya paliurus polysaccharides (CP) on carbon tetrachloride (CCl4) induced mice and explore its underlying mechanism.
32006582	0	0	theme	chitin	49:54	arg1	source					39:44	a new source	33:44	a new source of chitin and chitosan	33:67	Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp.
32006582	9	1	theme	Candida	1184:1190	arg1	albicans					1174:1181	Candida albicans	1166:1181	Candida albicans	1166:1181	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	9	1	theme	Candida	1184:1190	arg1	tropicalis					1192:1201	Candida tropicalis	1184:1201	Candida tropicalis	1184:1201	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	8	2	theme	clinical	948:955	arg1	strains					957:963	twenty isolated clinical strains	932:963	twenty isolated clinical strains of Candida yeast	932:980	The antifungal activity of the chitosan samples and the chitooligomer were tested toward twenty isolated clinical strains of Candida yeast.
32006582	1	3	theme	exoskeleton	209:219	arg1	composition					151:161	the chemical composition	138:161	the chemical composition of the Chilean freshwater crab Aegla cholchol exoskeleton	138:219	In the present work, the chemical composition of the Chilean freshwater crab Aegla cholchol exoskeleton was studied for the first time.
32006582	8	4	theme	isolated	939:946	arg1	strains					957:963	twenty isolated clinical strains	932:963	twenty isolated clinical strains of Candida yeast	932:980	The antifungal activity of the chitosan samples and the chitooligomer were tested toward twenty isolated clinical strains of Candida yeast.
32006582	8	5	theme	samples	883:889	arg1	chitooligomer					899:911	the chitooligomer	895:911	the chitooligomer	895:911	The antifungal activity of the chitosan samples and the chitooligomer were tested toward twenty isolated clinical strains of Candida yeast.
32006582	8	5	theme	samples	883:889	arg1	activity					858:865	The antifungal activity	843:865	The antifungal activity of the chitosan samples	843:889	The antifungal activity of the chitosan samples and the chitooligomer were tested toward twenty isolated clinical strains of Candida yeast.
32006582	9	6	theme	higher	1015:1020	arg1	weight					1032:1037	higher molecular weight	1015:1037	higher molecular weight	1015:1037	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	1	7	theme	present	124:130	arg1	work					132:135	the present work	120:135	the present work	120:135	In the present work, the chemical composition of the Chilean freshwater crab Aegla cholchol exoskeleton was studied for the first time.
32006582	10	8	theme	excellent	1290:1298	arg1	source					1308:1313	an excellent natural source	1287:1313	an excellent natural source for production of bioactive materials with potential applications in the health system	1287:1400	Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains.
32006582	10	8	theme	excellent	1290:1298	arg1	cholchol					1275:1282	Aegla cholchol	1269:1282	Aegla cholchol	1269:1282	Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains.
32006582	0	9	theme	chitosan	60:67	arg1	source					39:44	a new source	33:44	a new source of chitin and chitosan	33:67	Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp.
32006582	9	10	theme	lowest	1050:1055	arg1	MIC					1091:1093	MIC	1091:1093	MIC	1091:1093	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	9	10	theme	lowest	1050:1055	arg1	concentration					1076:1088	lowest minimum inhibitory concentration	1050:1088	the lowest minimum inhibitory concentration (MIC) values	1046:1101	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	9	11	theme	molecular	1022:1030	arg1	weight					1032:1037	higher molecular weight	1015:1037	higher molecular weight	1015:1037	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	9	12	theme	antifungal	1139:1148	arg1	activity					1150:1157	the highest antifungal activity	1127:1157	the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis	1127:1245	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	5	13	theme	Chemical	563:570	arg1	structure					572:580	Chemical structure	563:580	Chemical structure of chitin and chitosan	563:603	Chemical structure of chitin and chitosan was confirmed by NMR spectroscopy.
32006582	8	14	theme	antifungal	847:856	arg1	activity					858:865	The antifungal activity	843:865	The antifungal activity of the chitosan samples	843:889	The antifungal activity of the chitosan samples and the chitooligomer were tested toward twenty isolated clinical strains of Candida yeast.
32006582	3	15	theme	time	458:461	arg1	regimes					463:469	different temperature and time regimes	432:469	regimes	463:469	Moreover, chitosan was extracted by alkaline treatment at different temperature and time regimes.
32006582	9	16	theme	Candida	1204:1210	arg1	albicans					1174:1181	Candida albicans	1166:1181	Candida albicans	1166:1181	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	9	16	theme	Candida	1204:1210	arg1	glabrata					1212:1219	Candida glabrata	1204:1219	Candida glabrata	1204:1219	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	0	17	theme	antifungal	74:83	arg1	properties					85:94	antifungal properties	74:94	antifungal properties against Candida spp	74:114	Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp.
32006582	3	18	theme	different	432:440	arg1	temperature					442:452	different temperature and time regimes	432:469	temperature	442:452	Moreover, chitosan was extracted by alkaline treatment at different temperature and time regimes.
32006582	10	19	from	applications	1368:1379	arg1	system					1395:1400	the health system	1384:1400	the health system	1384:1400	Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains.
32006582	9	20	theme	lower	997:1001	arg1	DA					1003:1004	lower DA	997:1004	lower DA (4%)	997:1009	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	9	20	theme	lower	997:1001	arg1	%					1008:1008	4%	1007:1008	4%	1007:1008	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	9	21	theme	Candida	1226:1232	arg1	parapsilosis					1234:1245	Candida parapsilosis	1226:1245	Candida parapsilosis	1226:1245	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	9	21	theme	Candida	1226:1232	arg1	albicans					1174:1181	Candida albicans	1166:1181	Candida albicans	1166:1181	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	9	22	theme	Candida	1166:1172	arg1	parapsilosis					1234:1245	Candida parapsilosis	1226:1245	Candida parapsilosis	1226:1245	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	9	22	theme	Candida	1166:1172	arg1	albicans					1174:1181	Candida albicans	1166:1181	Candida albicans	1166:1181	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	9	22	theme	Candida	1166:1172	arg1	tropicalis					1192:1201	Candida tropicalis	1184:1201	Candida tropicalis	1184:1201	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	9	22	theme	Candida	1166:1172	arg1	glabrata					1212:1219	Candida glabrata	1204:1219	Candida glabrata	1204:1219	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	7	23	theme	obtained	710:717	arg1	samples					728:734	The obtained chitosan samples	706:734	The obtained chitosan samples	706:734	The obtained chitosan samples had a degree of N-acetylation (DA) between 4 and 15% and molecular weight (Mw) in the range of 65-201 kDa.
32006582	7	24	theme	N-acetylation	752:764	arg1	Mw					811:812	Mw	811:812	Mw	811:812	The obtained chitosan samples had a degree of N-acetylation (DA) between 4 and 15% and molecular weight (Mw) in the range of 65-201 kDa.
32006582	7	24	theme	N-acetylation	752:764	arg1	weight					803:808	molecular weight	793:808	molecular weight (Mw)	793:813	The obtained chitosan samples had a degree of N-acetylation (DA) between 4 and 15% and molecular weight (Mw) in the range of 65-201 kDa.
32006582	7	24	theme	N-acetylation	752:764	arg1	degree					742:747	a degree	740:747	a degree of N-acetylation (DA) between 4 and 15%	740:787	The obtained chitosan samples had a degree of N-acetylation (DA) between 4 and 15% and molecular weight (Mw) in the range of 65-201 kDa.
32006582	0	25	theme	Chilean	0:6	arg1	crab					8:11	Chilean crab	0:11	Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp.	0:115	Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp.
32006582	0	25	theme	Chilean	0:6	arg1	cholchol					20:27	Aegla cholchol	14:27	Aegla cholchol	14:27	Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp.
32006582	2	26	attach	isolated	266:273	arg1	parts					296:300	three main body parts	280:300	three main body parts (pincers, legs, carapace)	280:326	α-Chitin was isolated from three main body parts (pincers, legs, carapace), and its content ranged from 9.0-10.4% (w/w).
32006582	2	26	attach	isolated	266:273	arg2	α-Chitin					253:260	α-Chitin	253:260	α-Chitin	253:260	α-Chitin was isolated from three main body parts (pincers, legs, carapace), and its content ranged from 9.0-10.4% (w/w).
32006582	10	27	theme	natural	1300:1306	arg1	source					1308:1313	an excellent natural source	1287:1313	an excellent natural source for production of bioactive materials with potential applications in the health system	1287:1400	Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains.
32006582	10	27	theme	natural	1300:1306	arg1	cholchol					1275:1282	Aegla cholchol	1269:1282	Aegla cholchol	1269:1282	Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains.
32006582	8	28	theme	chitosan	874:881	arg1	samples					883:889	the chitosan samples	870:889	the chitosan samples	870:889	The antifungal activity of the chitosan samples and the chitooligomer were tested toward twenty isolated clinical strains of Candida yeast.
32006582	1	29	theme	chemical	142:149	arg1	composition					151:161	the chemical composition	138:161	the chemical composition of the Chilean freshwater crab Aegla cholchol exoskeleton	138:219	In the present work, the chemical composition of the Chilean freshwater crab Aegla cholchol exoskeleton was studied for the first time.
32006582	0	30	theme	Aegla	14:18	arg1	crab					8:11	Chilean crab	0:11	Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp.	0:115	Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp.
32006582	0	30	theme	Aegla	14:18	arg1	cholchol					20:27	Aegla cholchol	14:27	Aegla cholchol	14:27	Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp.
32006582	1	31	theme	first	241:245	arg1	time					247:250	the first time	237:250	the first time	237:250	In the present work, the chemical composition of the Chilean freshwater crab Aegla cholchol exoskeleton was studied for the first time.
32006582	5	32	theme	chitosan	596:603	arg1	structure					572:580	Chemical structure	563:580	Chemical structure of chitin and chitosan	563:603	Chemical structure of chitin and chitosan was confirmed by NMR spectroscopy.
32006582	9	33	theme	inhibitory	1065:1074	arg1	MIC					1091:1093	MIC	1091:1093	MIC	1091:1093	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	9	33	theme	inhibitory	1065:1074	arg1	concentration					1076:1088	lowest minimum inhibitory concentration	1050:1088	the lowest minimum inhibitory concentration (MIC) values	1046:1101	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	2	34	dep	parts	296:300	arg1	pincers					303:309	pincers	303:309	pincers	303:309	α-Chitin was isolated from three main body parts (pincers, legs, carapace), and its content ranged from 9.0-10.4% (w/w).
32006582	2	34	dep	parts	296:300	arg1	carapace					318:325	carapace	318:325	carapace	318:325	α-Chitin was isolated from three main body parts (pincers, legs, carapace), and its content ranged from 9.0-10.4% (w/w).
32006582	2	34	dep	parts	296:300	arg1	legs					312:315	legs	312:315	legs	312:315	α-Chitin was isolated from three main body parts (pincers, legs, carapace), and its content ranged from 9.0-10.4% (w/w).
32006582	9	35	theme	minimum	1057:1063	arg1	MIC					1091:1093	MIC	1091:1093	MIC	1091:1093	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	9	35	theme	minimum	1057:1063	arg1	concentration					1076:1088	lowest minimum inhibitory concentration	1050:1088	the lowest minimum inhibitory concentration (MIC) values	1046:1101	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	4	36	theme	cholchol	478:485	arg1	exoskeleton					487:497	Aegla cholchol exoskeleton	472:497	Aegla cholchol exoskeleton	472:497	Aegla cholchol exoskeleton, chitin and chitosans were characterized by FTIR, TGA, and SEM.
32006582	6	37	theme	weight	659:664	arg1	determinations					666:679	Chitosan molecular weight determinations	640:679	Chitosan molecular weight determinations	640:679	Chitosan molecular weight determinations were carried out by GPC.
32006582	9	38	theme	concentration	1076:1088	arg1	values					1096:1101	the lowest minimum inhibitory concentration (MIC) values	1046:1101	the lowest minimum inhibitory concentration (MIC) values	1046:1101	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	3	39	theme	alkaline	410:417	arg1	treatment					419:427	alkaline treatment	410:427	alkaline treatment	410:427	Moreover, chitosan was extracted by alkaline treatment at different temperature and time regimes.
32006582	10	40	theme	health	1388:1393	arg1	system					1395:1400	the health system	1384:1400	the health system	1384:1400	Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains.
32006582	4	41	theme	Aegla	472:476	arg1	exoskeleton					487:497	Aegla cholchol exoskeleton	472:497	Aegla cholchol exoskeleton	472:497	Aegla cholchol exoskeleton, chitin and chitosans were characterized by FTIR, TGA, and SEM.
32006582	10	42	theme	bioactive	1333:1341	arg1	materials					1343:1351	bioactive materials	1333:1351	bioactive materials with potential applications in the health system	1333:1400	Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains.
32006582	9	43	with	Chitosan	983:990	arg1	DA					1003:1004	lower DA	997:1004	lower DA (4%)	997:1009	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	9	43	with	Chitosan	983:990	arg1	weight					1032:1037	higher molecular weight	1015:1037	higher molecular weight	1015:1037	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	9	43	with	Chitosan	983:990	arg1	%					1008:1008	4%	1007:1008	4%	1007:1008	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	0	44	theme	Candida	104:110	arg1	spp					112:114	Candida spp	104:114	Candida spp	104:114	Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp.
32006582	1	45	theme	Chilean	170:176	arg1	exoskeleton					209:219	the Chilean freshwater crab Aegla cholchol exoskeleton	166:219	the Chilean freshwater crab Aegla cholchol exoskeleton	166:219	In the present work, the chemical composition of the Chilean freshwater crab Aegla cholchol exoskeleton was studied for the first time.
32006582	5	46	theme	NMR	622:624	arg1	spectroscopy					626:637	NMR spectroscopy	622:637	NMR spectroscopy	622:637	Chemical structure of chitin and chitosan was confirmed by NMR spectroscopy.
32006582	8	47	theme	yeast	976:980	arg1	strains					957:963	twenty isolated clinical strains	932:963	twenty isolated clinical strains of Candida yeast	932:980	The antifungal activity of the chitosan samples and the chitooligomer were tested toward twenty isolated clinical strains of Candida yeast.
32006582	7	48	theme	chitosan	719:726	arg1	samples					728:734	The obtained chitosan samples	706:734	The obtained chitosan samples	706:734	The obtained chitosan samples had a degree of N-acetylation (DA) between 4 and 15% and molecular weight (Mw) in the range of 65-201 kDa.
32006582	8	49	theme	Candida	968:974	arg1	yeast					976:980	Candida yeast	968:980	Candida yeast	968:980	The antifungal activity of the chitosan samples and the chitooligomer were tested toward twenty isolated clinical strains of Candida yeast.
32006582	9	50	theme	highest	1131:1137	arg1	activity					1150:1157	the highest antifungal activity	1127:1157	the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis	1127:1245	Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis.
32006582	7	51	contain	had	736:738	arg2	weight					803:808	molecular weight	793:808	molecular weight (Mw)	793:813	The obtained chitosan samples had a degree of N-acetylation (DA) between 4 and 15% and molecular weight (Mw) in the range of 65-201 kDa.
32006582	7	51	contain	had	736:738	arg1	samples					728:734	The obtained chitosan samples	706:734	The obtained chitosan samples	706:734	The obtained chitosan samples had a degree of N-acetylation (DA) between 4 and 15% and molecular weight (Mw) in the range of 65-201 kDa.
32006582	7	51	contain	had	736:738	arg2	degree					742:747	a degree	740:747	a degree of N-acetylation (DA) between 4 and 15%	740:787	The obtained chitosan samples had a degree of N-acetylation (DA) between 4 and 15% and molecular weight (Mw) in the range of 65-201 kDa.
32006582	7	51	contain	had	736:738	arg2	Mw					811:812	Mw	811:812	Mw	811:812	The obtained chitosan samples had a degree of N-acetylation (DA) between 4 and 15% and molecular weight (Mw) in the range of 65-201 kDa.
32006582	0	52	with	crab	8:11	arg1	properties					85:94	antifungal properties	74:94	antifungal properties against Candida spp	74:114	Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp.
32006582	2	53	theme	body	291:294	arg1	parts					296:300	three main body parts	280:300	three main body parts (pincers, legs, carapace)	280:326	α-Chitin was isolated from three main body parts (pincers, legs, carapace), and its content ranged from 9.0-10.4% (w/w).
32006582	5	54	theme	chitin	585:590	arg1	structure					572:580	Chemical structure	563:580	Chemical structure of chitin and chitosan	563:603	Chemical structure of chitin and chitosan was confirmed by NMR spectroscopy.
32006582	10	55	theme	Aegla	1269:1273	arg1	source					1308:1313	an excellent natural source	1287:1313	an excellent natural source for production of bioactive materials with potential applications in the health system	1287:1400	Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains.
32006582	10	55	theme	Aegla	1269:1273	arg1	cholchol					1275:1282	Aegla cholchol	1269:1282	Aegla cholchol	1269:1282	Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains.
32006582	6	56	theme	molecular	649:657	arg1	determinations					666:679	Chitosan molecular weight determinations	640:679	Chitosan molecular weight determinations	640:679	Chitosan molecular weight determinations were carried out by GPC.
32006582	2	57	theme	main	286:289	arg1	parts					296:300	three main body parts	280:300	three main body parts (pincers, legs, carapace)	280:326	α-Chitin was isolated from three main body parts (pincers, legs, carapace), and its content ranged from 9.0-10.4% (w/w).
32006582	7	58	theme	molecular	793:801	arg1	Mw					811:812	Mw	811:812	Mw	811:812	The obtained chitosan samples had a degree of N-acetylation (DA) between 4 and 15% and molecular weight (Mw) in the range of 65-201 kDa.
32006582	7	58	theme	molecular	793:801	arg1	weight					803:808	molecular weight	793:808	molecular weight (Mw)	793:813	The obtained chitosan samples had a degree of N-acetylation (DA) between 4 and 15% and molecular weight (Mw) in the range of 65-201 kDa.
32006582	1	59	theme	freshwater	178:187	arg1	exoskeleton					209:219	the Chilean freshwater crab Aegla cholchol exoskeleton	166:219	the Chilean freshwater crab Aegla cholchol exoskeleton	166:219	In the present work, the chemical composition of the Chilean freshwater crab Aegla cholchol exoskeleton was studied for the first time.
32006582	10	60	theme	potential	1358:1366	arg1	applications					1368:1379	potential applications	1358:1379	potential applications in the health system	1358:1400	Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains.
32006582	6	61	theme	Chitosan	640:647	arg1	determinations					666:679	Chitosan molecular weight determinations	640:679	Chitosan molecular weight determinations	640:679	Chitosan molecular weight determinations were carried out by GPC.
32006582	1	62	theme	crab	189:192	arg1	exoskeleton					209:219	the Chilean freshwater crab Aegla cholchol exoskeleton	166:219	the Chilean freshwater crab Aegla cholchol exoskeleton	166:219	In the present work, the chemical composition of the Chilean freshwater crab Aegla cholchol exoskeleton was studied for the first time.
32006582	10	63	theme	materials	1343:1351	arg1	production					1319:1328	production	1319:1328	production of bioactive materials with potential applications in the health system	1319:1400	Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains.
32006582	10	64	with	materials	1343:1351	arg1	applications					1368:1379	potential applications	1358:1379	potential applications in the health system	1358:1400	Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains.
32006582	1	65	theme	Aegla	194:198	arg1	exoskeleton					209:219	the Chilean freshwater crab Aegla cholchol exoskeleton	166:219	the Chilean freshwater crab Aegla cholchol exoskeleton	166:219	In the present work, the chemical composition of the Chilean freshwater crab Aegla cholchol exoskeleton was studied for the first time.
32006582	0	66	theme	new	35:37	arg1	source					39:44	a new source	33:44	a new source of chitin and chitosan	33:67	Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp.
32006582	10	67	theme	Candida	1441:1447	arg1	strains					1449:1455	Candida strains	1441:1455	Candida strains	1441:1455	Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains.
32006582	7	68	theme	65-201 kDa	831:840	arg1	range					822:826	the range	818:826	the range of 65-201 kDa	818:840	The obtained chitosan samples had a degree of N-acetylation (DA) between 4 and 15% and molecular weight (Mw) in the range of 65-201 kDa.
32006582	1	69	theme	cholchol	200:207	arg1	exoskeleton					209:219	the Chilean freshwater crab Aegla cholchol exoskeleton	166:219	the Chilean freshwater crab Aegla cholchol exoskeleton	166:219	In the present work, the chemical composition of the Chilean freshwater crab Aegla cholchol exoskeleton was studied for the first time.
34853926	8	0	theme	Pb	1353:1354	arg1	assessment					1306:1315	The isotherm model assessment	1287:1315	The isotherm model assessment for adsorption experimental data of Pb(II)	1287:1358	The isotherm model assessment for adsorption experimental data of Pb(II) showed the best fit for the Langmuir model on using GG.
34853926	6	1	theme	adsorption	1127:1136	arg1	tests					1138:1142	adsorption tests	1127:1142	adsorption tests beyond 3.0 pH	1127:1156	The experimental results showed that the optimum removal of Pb(II) ions was high at a pH of 3.0 for all blends, and adsorption tests beyond 3.0 pH demonstrated a decline in adsorption performance.
34853926	4	2	theme	guar	831:834	arg1	gum					836:838	guar gum	831:838	guar gum	831:838	Batch adsorption tests were performed to evaluate the adsorption capacity of UBS and the GGBS with various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum (GG) towards the removal of Pb(II) ions.
34853926	9	3	theme	guar	1459:1462	arg1	material					1583:1590	sustainable liner material	1565:1590	sustainable liner material in sanitary landfills	1565:1612	The present research demonstrated that the guar gum-treated blends exhibited potential Pb(II) ion adsorption properties and therefore can be used as sustainable liner material in sanitary landfills.
34853926	9	3	theme	guar	1459:1462	arg1	blends					1476:1481	the guar gum-treated blends	1455:1481	the guar gum-treated blends	1455:1481	The present research demonstrated that the guar gum-treated blends exhibited potential Pb(II) ion adsorption properties and therefore can be used as sustainable liner material in sanitary landfills.
34853926	7	4	theme	%	1251:1251	arg1	efficiency					1235:1244	The maximum Pb(II) removal efficiency	1208:1244	The maximum Pb(II) removal efficiency of 95%	1208:1251	The maximum Pb(II) removal efficiency of 95% was obtained using the 2.0% GGBS.
34853926	7	5	theme	2.0	1276:1278	arg1	%					1279:1279	%	1279:1279	%	1279:1279	The maximum Pb(II) removal efficiency of 95% was obtained using the 2.0% GGBS.
34853926	4	6	theme	gum	836:838	arg1	proportions					788:798	various proportions	780:798	various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum	780:838	Batch adsorption tests were performed to evaluate the adsorption capacity of UBS and the GGBS with various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum (GG) towards the removal of Pb(II) ions.
34853926	4	7	theme	Batch	681:685	arg1	tests					698:702	Batch adsorption tests	681:702	Batch adsorption tests	681:702	Batch adsorption tests were performed to evaluate the adsorption capacity of UBS and the GGBS with various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum (GG) towards the removal of Pb(II) ions.
34853926	6	8	theme	3.0	1103:1105	arg1	pH					1097:1098	a pH	1095:1098	a pH of 3.0 for all blends	1095:1120	The experimental results showed that the optimum removal of Pb(II) ions was high at a pH of 3.0 for all blends, and adsorption tests beyond 3.0 pH demonstrated a decline in adsorption performance.
34853926	2	9	theme	Characterization	296:311	arg1	fluorescence					357:368	X-ray fluorescence	351:368	X-ray fluorescence	351:368	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	2	9	theme	Characterization	296:311	arg1	Fourier					371:377	Fourier	371:377	Fourier transform infrared spectroscopy	371:409	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	2	9	theme	Characterization	296:311	arg1	microscope					433:442	scanning electron microscope	415:442	scanning electron microscope	415:442	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	2	9	theme	Characterization	296:311	arg1	diffraction					338:348	X-ray diffraction	332:348	X-ray diffraction	332:348	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	2	9	theme	Characterization	296:311	arg1	techniques					313:322	Characterization techniques	296:322	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope	296:442	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	1	10	theme	advantageous	173:184	arg1	characteristics					198:212	potentially advantageous engineering characteristics	161:212	potentially advantageous engineering characteristics of hydraulic conductivity and strength for a soil to be used as a liner material	161:293	Guar gum blended soil (GGBS) offers potentially advantageous engineering characteristics of hydraulic conductivity and strength for a soil to be used as a liner material.
34853926	9	11	theme	potential	1493:1501	arg1	II					1506:1507	II	1506:1507	II	1506:1507	The present research demonstrated that the guar gum-treated blends exhibited potential Pb(II) ion adsorption properties and therefore can be used as sustainable liner material in sanitary landfills.
34853926	9	11	theme	potential	1493:1501	arg1	Pb					1503:1504	potential Pb	1493:1504	potential Pb(II) ion adsorption properties	1493:1534	The present research demonstrated that the guar gum-treated blends exhibited potential Pb(II) ion adsorption properties and therefore can be used as sustainable liner material in sanitary landfills.
34853926	7	12	theme	removal	1227:1233	arg1	efficiency					1235:1244	The maximum Pb(II) removal efficiency	1208:1244	The maximum Pb(II) removal efficiency of 95%	1208:1251	The maximum Pb(II) removal efficiency of 95% was obtained using the 2.0% GGBS.
34853926	4	13	with	GGBS	770:773	arg1	proportions					788:798	various proportions	780:798	various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum	780:838	Batch adsorption tests were performed to evaluate the adsorption capacity of UBS and the GGBS with various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum (GG) towards the removal of Pb(II) ions.
34853926	5	14	theme	metal	982:986	arg1	ions					988:991	metal ions	982:991	metal ions	982:991	Batch adsorption experiments were conducted by varying the pH, dosage of adsorbent, concentration of metal ions and contact time.
34853926	1	15	theme	hydraulic	217:225	arg1	conductivity					227:238	conductivity	227:238	conductivity	227:238	Guar gum blended soil (GGBS) offers potentially advantageous engineering characteristics of hydraulic conductivity and strength for a soil to be used as a liner material.
34853926	7	16	theme	Pb	1220:1221	arg1	efficiency					1235:1244	The maximum Pb(II) removal efficiency	1208:1244	The maximum Pb(II) removal efficiency of 95%	1208:1251	The maximum Pb(II) removal efficiency of 95% was obtained using the 2.0% GGBS.
34853926	9	17	theme	adsorption	1514:1523	arg1	properties					1525:1534	potential Pb(II) ion adsorption properties	1493:1534	potential Pb(II) ion adsorption properties	1493:1534	The present research demonstrated that the guar gum-treated blends exhibited potential Pb(II) ion adsorption properties and therefore can be used as sustainable liner material in sanitary landfills.
34853926	2	18	from	changes	526:532	arg1	UBS					557:559	UBS	557:559	UBS	557:559	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	2	18	from	changes	526:532	arg1	GGBS					566:569	GGBS	566:569	GGBS	566:569	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	2	18	from	changes	526:532	arg1	soil					551:554	the unblended soil	537:554	the unblended soil (UBS)	537:560	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	4	19	theme	adsorption	735:744	arg1	capacity					746:753	the adsorption capacity	731:753	the adsorption capacity of UBS and the GGBS with various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum (GG)	731:843	Batch adsorption tests were performed to evaluate the adsorption capacity of UBS and the GGBS with various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum (GG) towards the removal of Pb(II) ions.
34853926	5	20	theme	adsorbent	954:962	arg1	dosage					944:949	dosage	944:949	dosage of adsorbent	944:962	Batch adsorption experiments were conducted by varying the pH, dosage of adsorbent, concentration of metal ions and contact time.
34853926	5	20	theme	adsorbent	954:962	arg1	pH					940:941	pH	940:941	pH	940:941	Batch adsorption experiments were conducted by varying the pH, dosage of adsorbent, concentration of metal ions and contact time.
34853926	3	21	used	used	610:613	arg2	approaches					595:604	These characterization approaches	572:604	These characterization approaches	572:604	These characterization approaches are used to understand adsorption-associated mechanisms of Pb(II) removal.
34853926	2	22	theme	morphological	512:524	arg1	changes					526:532	morphological changes	512:532	morphological changes in the unblended soil (UBS) and GGBS	512:569	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	8	23	theme	isotherm	1291:1298	arg1	assessment					1306:1315	The isotherm model assessment	1287:1315	The isotherm model assessment for adsorption experimental data of Pb(II)	1287:1358	The isotherm model assessment for adsorption experimental data of Pb(II) showed the best fit for the Langmuir model on using GG.
34853926	6	24	theme	ions	1078:1081	arg1	high					1087:1090	high	1087:1090	high	1087:1090	The experimental results showed that the optimum removal of Pb(II) ions was high at a pH of 3.0 for all blends, and adsorption tests beyond 3.0 pH demonstrated a decline in adsorption performance.
34853926	6	24	theme	ions	1078:1081	arg1	removal					1060:1066	the optimum removal	1048:1066	the optimum removal of Pb(II) ions	1048:1081	The experimental results showed that the optimum removal of Pb(II) ions was high at a pH of 3.0 for all blends, and adsorption tests beyond 3.0 pH demonstrated a decline in adsorption performance.
34853926	6	25	from	pH	1097:1098	arg1	removal					1060:1066	the optimum removal	1048:1066	the optimum removal of Pb(II) ions	1048:1081	The experimental results showed that the optimum removal of Pb(II) ions was high at a pH of 3.0 for all blends, and adsorption tests beyond 3.0 pH demonstrated a decline in adsorption performance.
34853926	6	25	from	pH	1097:1098	arg1	high					1087:1090	high	1087:1090	high	1087:1090	The experimental results showed that the optimum removal of Pb(II) ions was high at a pH of 3.0 for all blends, and adsorption tests beyond 3.0 pH demonstrated a decline in adsorption performance.
34853926	9	26	used	used	1557:1560	arg2	material					1583:1590	sustainable liner material	1565:1590	sustainable liner material in sanitary landfills	1565:1612	The present research demonstrated that the guar gum-treated blends exhibited potential Pb(II) ion adsorption properties and therefore can be used as sustainable liner material in sanitary landfills.
34853926	9	26	used	used	1557:1560	arg2	blends					1476:1481	the guar gum-treated blends	1455:1481	the guar gum-treated blends	1455:1481	The present research demonstrated that the guar gum-treated blends exhibited potential Pb(II) ion adsorption properties and therefore can be used as sustainable liner material in sanitary landfills.
34853926	0	27	theme	gum	87:89	arg1	characteristics					63:77	mineralogical and adsorption characteristics	34:77	mineralogical and adsorption characteristics of guar gum	34:89	Understanding the microstructure, mineralogical and adsorption characteristics of guar gum blended soil as a liner material.
34853926	2	28	theme	unblended	541:549	arg1	UBS					557:559	UBS	557:559	UBS	557:559	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	2	28	theme	unblended	541:549	arg1	soil					551:554	the unblended soil	537:554	the unblended soil (UBS)	537:560	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	8	29	theme	adsorption	1321:1330	arg1	data					1345:1348	adsorption experimental data	1321:1348	adsorption experimental data	1321:1348	The isotherm model assessment for adsorption experimental data of Pb(II) showed the best fit for the Langmuir model on using GG.
34853926	4	30	theme	GGBS	770:773	arg1	capacity					746:753	the adsorption capacity	731:753	the adsorption capacity of UBS and the GGBS with various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum (GG)	731:843	Batch adsorption tests were performed to evaluate the adsorption capacity of UBS and the GGBS with various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum (GG) towards the removal of Pb(II) ions.
34853926	9	31	theme	sustainable	1565:1575	arg1	material					1583:1590	sustainable liner material	1565:1590	sustainable liner material in sanitary landfills	1565:1612	The present research demonstrated that the guar gum-treated blends exhibited potential Pb(II) ion adsorption properties and therefore can be used as sustainable liner material in sanitary landfills.
34853926	9	31	theme	sustainable	1565:1575	arg1	blends					1476:1481	the guar gum-treated blends	1455:1481	the guar gum-treated blends	1455:1481	The present research demonstrated that the guar gum-treated blends exhibited potential Pb(II) ion adsorption properties and therefore can be used as sustainable liner material in sanitary landfills.
34853926	4	32	theme	various	780:786	arg1	proportions					788:798	various proportions	780:798	various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum	780:838	Batch adsorption tests were performed to evaluate the adsorption capacity of UBS and the GGBS with various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum (GG) towards the removal of Pb(II) ions.
34853926	5	33	theme	time	1005:1008	arg1	concentration					965:977	the pH, dosage of adsorbent, concentration	936:977	the pH, dosage of adsorbent, concentration of metal ions and contact time	936:1008	Batch adsorption experiments were conducted by varying the pH, dosage of adsorbent, concentration of metal ions and contact time.
34853926	5	34	theme	adsorption	887:896	arg1	experiments					898:908	Batch adsorption experiments	881:908	Batch adsorption experiments	881:908	Batch adsorption experiments were conducted by varying the pH, dosage of adsorbent, concentration of metal ions and contact time.
34853926	2	35	from	composition	477:487	arg1	UBS					557:559	UBS	557:559	UBS	557:559	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	2	35	from	composition	477:487	arg1	GGBS					566:569	GGBS	566:569	GGBS	566:569	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	2	35	from	composition	477:487	arg1	soil					551:554	the unblended soil	537:554	the unblended soil (UBS)	537:560	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	6	36	from	decline	1173:1179	arg1	performance					1195:1205	adsorption performance	1184:1205	adsorption performance	1184:1205	The experimental results showed that the optimum removal of Pb(II) ions was high at a pH of 3.0 for all blends, and adsorption tests beyond 3.0 pH demonstrated a decline in adsorption performance.
34853926	9	37	theme	sanitary	1595:1602	arg1	landfills					1604:1612	sanitary landfills	1595:1612	sanitary landfills	1595:1612	The present research demonstrated that the guar gum-treated blends exhibited potential Pb(II) ion adsorption properties and therefore can be used as sustainable liner material in sanitary landfills.
34853926	6	38	theme	Pb	1071:1072	arg1	ions					1078:1081	Pb(II) ions	1071:1081	Pb(II) ions	1071:1081	The experimental results showed that the optimum removal of Pb(II) ions was high at a pH of 3.0 for all blends, and adsorption tests beyond 3.0 pH demonstrated a decline in adsorption performance.
34853926	2	39	theme	scanning	415:422	arg1	microscope					433:442	scanning electron microscope	415:442	scanning electron microscope	415:442	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	0	40	theme	mineralogical	34:46	arg1	characteristics					63:77	mineralogical and adsorption characteristics	34:77	mineralogical and adsorption characteristics of guar gum	34:89	Understanding the microstructure, mineralogical and adsorption characteristics of guar gum blended soil as a liner material.
34853926	2	41	theme	mineral	469:475	arg1	composition					477:487	the mineral composition	465:487	the mineral composition	465:487	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	8	42	theme	best	1371:1374	arg1	fit					1376:1378	the best fit	1367:1378	the best fit for the Langmuir model	1367:1401	The isotherm model assessment for adsorption experimental data of Pb(II) showed the best fit for the Langmuir model on using GG.
34853926	3	43	theme	Pb	665:666	arg1	removal					672:678	Pb(II) removal	665:678	Pb(II) removal	665:678	These characterization approaches are used to understand adsorption-associated mechanisms of Pb(II) removal.
34853926	0	44	theme	adsorption	52:61	arg1	characteristics					63:77	mineralogical and adsorption characteristics	34:77	mineralogical and adsorption characteristics of guar gum	34:89	Understanding the microstructure, mineralogical and adsorption characteristics of guar gum blended soil as a liner material.
34853926	2	45	dep	transform	379:387	arg1	infrared					389:396	infrared	389:396	transform infrared spectroscopy	379:409	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	1	46	theme	conductivity	227:238	arg1	characteristics					198:212	potentially advantageous engineering characteristics	161:212	potentially advantageous engineering characteristics of hydraulic conductivity and strength for a soil to be used as a liner material	161:293	Guar gum blended soil (GGBS) offers potentially advantageous engineering characteristics of hydraulic conductivity and strength for a soil to be used as a liner material.
34853926	8	47	theme	Langmuir	1388:1395	arg1	model					1397:1401	the Langmuir model	1384:1401	the Langmuir model	1384:1401	The isotherm model assessment for adsorption experimental data of Pb(II) showed the best fit for the Langmuir model on using GG.
34853926	3	48	theme	removal	672:678	arg1	mechanisms					651:660	adsorption-associated mechanisms	629:660	adsorption-associated mechanisms of Pb(II) removal	629:678	These characterization approaches are used to understand adsorption-associated mechanisms of Pb(II) removal.
34853926	1	49	theme	strength	244:251	arg1	characteristics					198:212	potentially advantageous engineering characteristics	161:212	potentially advantageous engineering characteristics of hydraulic conductivity and strength for a soil to be used as a liner material	161:293	Guar gum blended soil (GGBS) offers potentially advantageous engineering characteristics of hydraulic conductivity and strength for a soil to be used as a liner material.
34853926	9	50	theme	present	1420:1426	arg1	research					1428:1435	The present research	1416:1435	The present research	1416:1435	The present research demonstrated that the guar gum-treated blends exhibited potential Pb(II) ion adsorption properties and therefore can be used as sustainable liner material in sanitary landfills.
34853926	2	51	theme	X-ray	351:355	arg1	fluorescence					357:368	X-ray fluorescence	351:368	X-ray fluorescence	351:368	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	7	52	theme	%	1279:1279	arg1	GGBS					1281:1284	the 2.0% GGBS	1272:1284	the 2.0% GGBS	1272:1284	The maximum Pb(II) removal efficiency of 95% was obtained using the 2.0% GGBS.
34853926	2	53	used	used	449:452	arg2	techniques					313:322	Characterization techniques	296:322	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope	296:442	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	2	53	used	used	449:452	arg2	microscope					433:442	scanning electron microscope	415:442	scanning electron microscope	415:442	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	2	53	used	used	449:452	arg2	fluorescence					357:368	X-ray fluorescence	351:368	X-ray fluorescence	351:368	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	2	53	used	used	449:452	arg2	diffraction					338:348	X-ray diffraction	332:348	X-ray diffraction	332:348	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	2	53	used	used	449:452	arg2	Fourier					371:377	Fourier	371:377	Fourier transform infrared spectroscopy	371:409	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	9	54	from	material	1583:1590	arg1	landfills					1604:1612	sanitary landfills	1595:1612	sanitary landfills	1595:1612	The present research demonstrated that the guar gum-treated blends exhibited potential Pb(II) ion adsorption properties and therefore can be used as sustainable liner material in sanitary landfills.
34853926	9	55	theme	gum-treated	1464:1474	arg1	material					1583:1590	sustainable liner material	1565:1590	sustainable liner material in sanitary landfills	1565:1612	The present research demonstrated that the guar gum-treated blends exhibited potential Pb(II) ion adsorption properties and therefore can be used as sustainable liner material in sanitary landfills.
34853926	9	55	theme	gum-treated	1464:1474	arg1	blends					1476:1481	the guar gum-treated blends	1455:1481	the guar gum-treated blends	1455:1481	The present research demonstrated that the guar gum-treated blends exhibited potential Pb(II) ion adsorption properties and therefore can be used as sustainable liner material in sanitary landfills.
34853926	6	56	theme	optimum	1052:1058	arg1	high					1087:1090	high	1087:1090	high	1087:1090	The experimental results showed that the optimum removal of Pb(II) ions was high at a pH of 3.0 for all blends, and adsorption tests beyond 3.0 pH demonstrated a decline in adsorption performance.
34853926	6	56	theme	optimum	1052:1058	arg1	removal					1060:1066	the optimum removal	1048:1066	the optimum removal of Pb(II) ions	1048:1081	The experimental results showed that the optimum removal of Pb(II) ions was high at a pH of 3.0 for all blends, and adsorption tests beyond 3.0 pH demonstrated a decline in adsorption performance.
34853926	6	57	theme	adsorption	1184:1193	arg1	performance					1195:1205	adsorption performance	1184:1205	adsorption performance	1184:1205	The experimental results showed that the optimum removal of Pb(II) ions was high at a pH of 3.0 for all blends, and adsorption tests beyond 3.0 pH demonstrated a decline in adsorption performance.
34853926	1	58	theme	engineering	186:196	arg1	characteristics					198:212	potentially advantageous engineering characteristics	161:212	potentially advantageous engineering characteristics of hydraulic conductivity and strength for a soil to be used as a liner material	161:293	Guar gum blended soil (GGBS) offers potentially advantageous engineering characteristics of hydraulic conductivity and strength for a soil to be used as a liner material.
34853926	9	59	theme	Pb	1503:1504	arg1	properties					1525:1534	potential Pb(II) ion adsorption properties	1493:1534	potential Pb(II) ion adsorption properties	1493:1534	The present research demonstrated that the guar gum-treated blends exhibited potential Pb(II) ion adsorption properties and therefore can be used as sustainable liner material in sanitary landfills.
34853926	2	60	from	groups	501:506	arg1	UBS					557:559	UBS	557:559	UBS	557:559	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	2	60	from	groups	501:506	arg1	GGBS					566:569	GGBS	566:569	GGBS	566:569	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	2	60	from	groups	501:506	arg1	soil					551:554	the unblended soil	537:554	the unblended soil (UBS)	537:560	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	4	61	dep	proportions	788:798	arg1	%					804:804	0.5%	801:804	0.5%	801:804	Batch adsorption tests were performed to evaluate the adsorption capacity of UBS and the GGBS with various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum (GG) towards the removal of Pb(II) ions.
34853926	4	61	dep	proportions	788:798	arg1	%					810:810	1.0%	807:810	1.0%	807:810	Batch adsorption tests were performed to evaluate the adsorption capacity of UBS and the GGBS with various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum (GG) towards the removal of Pb(II) ions.
34853926	4	61	dep	proportions	788:798	arg1	%					816:816	1.5%	813:816	1.5%	813:816	Batch adsorption tests were performed to evaluate the adsorption capacity of UBS and the GGBS with various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum (GG) towards the removal of Pb(II) ions.
34853926	4	61	dep	proportions	788:798	arg1	%					825:825	2.0%	822:825	2.0%	822:825	Batch adsorption tests were performed to evaluate the adsorption capacity of UBS and the GGBS with various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum (GG) towards the removal of Pb(II) ions.
34853926	4	62	with	UBS	758:760	arg1	proportions					788:798	various proportions	780:798	various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum	780:838	Batch adsorption tests were performed to evaluate the adsorption capacity of UBS and the GGBS with various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum (GG) towards the removal of Pb(II) ions.
34853926	5	63	theme	ions	988:991	arg1	concentration					965:977	the pH, dosage of adsorbent, concentration	936:977	the pH, dosage of adsorbent, concentration of metal ions and contact time	936:1008	Batch adsorption experiments were conducted by varying the pH, dosage of adsorbent, concentration of metal ions and contact time.
34853926	3	64	theme	characterization	578:593	arg1	approaches					595:604	These characterization approaches	572:604	These characterization approaches	572:604	These characterization approaches are used to understand adsorption-associated mechanisms of Pb(II) removal.
34853926	9	65	theme	ion	1510:1512	arg1	properties					1525:1534	potential Pb(II) ion adsorption properties	1493:1534	potential Pb(II) ion adsorption properties	1493:1534	The present research demonstrated that the guar gum-treated blends exhibited potential Pb(II) ion adsorption properties and therefore can be used as sustainable liner material in sanitary landfills.
34853926	3	66	theme	adsorption-associated	629:649	arg1	mechanisms					651:660	adsorption-associated mechanisms	629:660	adsorption-associated mechanisms of Pb(II) removal	629:678	These characterization approaches are used to understand adsorption-associated mechanisms of Pb(II) removal.
34853926	1	67	theme	Guar	125:128	arg1	gum					130:132	Guar gum blended soil (GGBS)	125:152	Guar gum blended soil (GGBS)	125:152	Guar gum blended soil (GGBS) offers potentially advantageous engineering characteristics of hydraulic conductivity and strength for a soil to be used as a liner material.
34853926	8	68	theme	model	1300:1304	arg1	assessment					1306:1315	The isotherm model assessment	1287:1315	The isotherm model assessment for adsorption experimental data of Pb(II)	1287:1358	The isotherm model assessment for adsorption experimental data of Pb(II) showed the best fit for the Langmuir model on using GG.
34853926	7	69	theme	maximum	1212:1218	arg1	efficiency					1235:1244	The maximum Pb(II) removal efficiency	1208:1244	The maximum Pb(II) removal efficiency of 95%	1208:1251	The maximum Pb(II) removal efficiency of 95% was obtained using the 2.0% GGBS.
34853926	0	70	theme	guar	82:85	arg1	gum					87:89	guar gum	82:89	guar gum	82:89	Understanding the microstructure, mineralogical and adsorption characteristics of guar gum blended soil as a liner material.
34853926	1	71	theme	blended	134:140	arg1	soil					142:145	blended soil	134:145	Guar gum blended soil (GGBS)	125:152	Guar gum blended soil (GGBS) offers potentially advantageous engineering characteristics of hydraulic conductivity and strength for a soil to be used as a liner material.
34853926	1	71	theme	blended	134:140	arg1	GGBS					148:151	GGBS	148:151	GGBS	148:151	Guar gum blended soil (GGBS) offers potentially advantageous engineering characteristics of hydraulic conductivity and strength for a soil to be used as a liner material.
34853926	4	72	theme	ions	875:878	arg1	removal					857:863	the removal	853:863	the removal of Pb(II) ions	853:878	Batch adsorption tests were performed to evaluate the adsorption capacity of UBS and the GGBS with various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum (GG) towards the removal of Pb(II) ions.
34853926	4	73	theme	adsorption	687:696	arg1	tests					698:702	Batch adsorption tests	681:702	Batch adsorption tests	681:702	Batch adsorption tests were performed to evaluate the adsorption capacity of UBS and the GGBS with various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum (GG) towards the removal of Pb(II) ions.
34853926	2	74	theme	functional	490:499	arg1	groups					501:506	functional groups	490:506	functional groups	490:506	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	8	75	theme	experimental	1332:1343	arg1	data					1345:1348	adsorption experimental data	1321:1348	adsorption experimental data	1321:1348	The isotherm model assessment for adsorption experimental data of Pb(II) showed the best fit for the Langmuir model on using GG.
34853926	4	76	theme	Pb	868:869	arg1	ions					875:878	Pb(II) ions	868:878	Pb(II) ions	868:878	Batch adsorption tests were performed to evaluate the adsorption capacity of UBS and the GGBS with various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum (GG) towards the removal of Pb(II) ions.
34853926	1	77	dep	gum	130:132	arg1	soil					142:145	blended soil	134:145	Guar gum blended soil (GGBS)	125:152	Guar gum blended soil (GGBS) offers potentially advantageous engineering characteristics of hydraulic conductivity and strength for a soil to be used as a liner material.
34853926	1	77	dep	gum	130:132	arg1	GGBS					148:151	GGBS	148:151	GGBS	148:151	Guar gum blended soil (GGBS) offers potentially advantageous engineering characteristics of hydraulic conductivity and strength for a soil to be used as a liner material.
34853926	0	78	theme	liner	109:113	arg1	material					115:122	a liner material	107:122	a liner material	107:122	Understanding the microstructure, mineralogical and adsorption characteristics of guar gum blended soil as a liner material.
34853926	5	79	theme	contact	997:1003	arg1	time					1005:1008	contact time	997:1008	contact time	997:1008	Batch adsorption experiments were conducted by varying the pH, dosage of adsorbent, concentration of metal ions and contact time.
34853926	5	80	theme	Batch	881:885	arg1	experiments					898:908	Batch adsorption experiments	881:908	Batch adsorption experiments	881:908	Batch adsorption experiments were conducted by varying the pH, dosage of adsorbent, concentration of metal ions and contact time.
34853926	9	81	theme	liner	1577:1581	arg1	material					1583:1590	sustainable liner material	1565:1590	sustainable liner material in sanitary landfills	1565:1612	The present research demonstrated that the guar gum-treated blends exhibited potential Pb(II) ion adsorption properties and therefore can be used as sustainable liner material in sanitary landfills.
34853926	9	81	theme	liner	1577:1581	arg1	blends					1476:1481	the guar gum-treated blends	1455:1481	the guar gum-treated blends	1455:1481	The present research demonstrated that the guar gum-treated blends exhibited potential Pb(II) ion adsorption properties and therefore can be used as sustainable liner material in sanitary landfills.
34853926	4	82	theme	UBS	758:760	arg1	capacity					746:753	the adsorption capacity	731:753	the adsorption capacity of UBS and the GGBS with various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum (GG)	731:843	Batch adsorption tests were performed to evaluate the adsorption capacity of UBS and the GGBS with various proportions (0.5%, 1.0%, 1.5% and 2.0%) of guar gum (GG) towards the removal of Pb(II) ions.
34853926	2	83	theme	electron	424:431	arg1	microscope					433:442	scanning electron microscope	415:442	scanning electron microscope	415:442	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	1	84	theme	liner	280:284	arg1	material					286:293	a liner material	278:293	a liner material	278:293	Guar gum blended soil (GGBS) offers potentially advantageous engineering characteristics of hydraulic conductivity and strength for a soil to be used as a liner material.
34853926	5	85	theme	pH	940:941	arg1	concentration					965:977	the pH, dosage of adsorbent, concentration	936:977	the pH, dosage of adsorbent, concentration of metal ions and contact time	936:1008	Batch adsorption experiments were conducted by varying the pH, dosage of adsorbent, concentration of metal ions and contact time.
34853926	2	86	theme	X-ray	332:336	arg1	diffraction					338:348	X-ray diffraction	332:348	X-ray diffraction	332:348	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34853926	6	87	theme	experimental	1015:1026	arg1	results					1028:1034	The experimental results	1011:1034	The experimental results	1011:1034	The experimental results showed that the optimum removal of Pb(II) ions was high at a pH of 3.0 for all blends, and adsorption tests beyond 3.0 pH demonstrated a decline in adsorption performance.
34853926	2	88	dep	Fourier	371:377	arg1	transform					379:387	transform	379:387	transform infrared spectroscopy	379:409	Characterization techniques such as X-ray diffraction, X-ray fluorescence, Fourier transform infrared spectroscopy and scanning electron microscope were used to examine the mineral composition, functional groups and morphological changes in the unblended soil (UBS) and GGBS.
34499468	6	0	theme	PGO	947:949	arg1	layer					951:955	a PGO layer	945:955	a PGO layer onto the wood scaffolds	945:979	The thermal conductivity of the nanocomposite is improved significantly by coating a PGO layer onto the wood scaffolds.
34499468	4	1	theme	wood	580:583	arg1	walls					590:594	thin wood cell walls	575:594	thin wood cell walls	575:594	The highly aligned porous microstructures, with thin wood cell walls and large voids along the cellulose microchannels, allow polymers to enter, resulting in the fabrication of the wood-polymer nanocomposite.
34499468	7	2	theme	volume	1163:1168	arg1	resistivity					1170:1180	volume resistivity	1163:1180	volume resistivity of about 1015 Ω·cm	1163:1199	The nanocomposite exhibits not only ultrahigh thermal conductivity (in-plane about 5.5 W m-1 K-1 and through-plane about 2.1 W m-1 K-1) but also satisfactory electrical insulation (volume resistivity of about 1015 Ω·cm).
34499468	7	3	theme	W	1107:1107	arg1	K-1					1113:1115	2.1 W m-1 K-1	1103:1115	2.1 W m-1 K-1	1103:1115	The nanocomposite exhibits not only ultrahigh thermal conductivity (in-plane about 5.5 W m-1 K-1 and through-plane about 2.1 W m-1 K-1) but also satisfactory electrical insulation (volume resistivity of about 1015 Ω·cm).
34499468	8	4	theme	thermal	1258:1264	arg1	materials					1277:1285	thermal management materials	1258:1285	thermal management materials	1258:1285	Therefore, the results provide a strategy to fabricate thermal management materials with excellent mechanical properties.
34499468	5	5	theme	nanocomposite	788:800	arg1	stiffness					748:756	The tensile stiffness	736:756	The tensile stiffness	736:756	The tensile stiffness and strength of the resulting nanocomposite reach 8.10 GPa and 90.3 MPa with a toughness of 5.0 MJ m-3.
34499468	5	5	theme	nanocomposite	788:800	arg1	strength					762:769	strength	762:769	strength of the resulting nanocomposite	762:800	The tensile stiffness and strength of the resulting nanocomposite reach 8.10 GPa and 90.3 MPa with a toughness of 5.0 MJ m-3.
34499468	4	6	theme	thin	575:578	arg1	walls					590:594	thin wood cell walls	575:594	thin wood cell walls	575:594	The highly aligned porous microstructures, with thin wood cell walls and large voids along the cellulose microchannels, allow polymers to enter, resulting in the fabrication of the wood-polymer nanocomposite.
34499468	7	7	theme	electrical	1140:1149	arg1	insulation					1151:1160	satisfactory electrical insulation	1127:1160	not only ultrahigh thermal conductivity (in-plane about 5.5 W m-1 K-1 and through-plane about 2.1 W m-1 K-1) but also satisfactory electrical insulation (volume resistivity of about 1015 Ω·cm)	1009:1200	The nanocomposite exhibits not only ultrahigh thermal conductivity (in-plane about 5.5 W m-1 K-1 and through-plane about 2.1 W m-1 K-1) but also satisfactory electrical insulation (volume resistivity of about 1015 Ω·cm).
34499468	7	8	theme	ultrahigh	1018:1026	arg1	conductivity					1036:1047	ultrahigh thermal conductivity	1018:1047	not only ultrahigh thermal conductivity (in-plane about 5.5 W m-1 K-1 and through-plane about 2.1 W m-1 K-1) but also satisfactory electrical insulation (volume resistivity of about 1015 Ω·cm)	1009:1200	The nanocomposite exhibits not only ultrahigh thermal conductivity (in-plane about 5.5 W m-1 K-1 and through-plane about 2.1 W m-1 K-1) but also satisfactory electrical insulation (volume resistivity of about 1015 Ω·cm).
34499468	3	9	theme	wood	409:412	arg1	microchannels					414:426	The wood microchannels	405:426	The wood microchannels	405:426	The wood microchannels were coated with polydopamine-surface-modified small graphene oxide (PGO) nanosheets via assembly.
34499468	4	10	theme	aligned	538:544	arg1	microstructures					553:567	The highly aligned porous microstructures	527:567	The highly aligned porous microstructures	527:567	The highly aligned porous microstructures, with thin wood cell walls and large voids along the cellulose microchannels, allow polymers to enter, resulting in the fabrication of the wood-polymer nanocomposite.
34499468	7	11	dep	K-1	1075:1077	arg1	W					1069:1069	W	1069:1069	W	1069:1069	The nanocomposite exhibits not only ultrahigh thermal conductivity (in-plane about 5.5 W m-1 K-1 and through-plane about 2.1 W m-1 K-1) but also satisfactory electrical insulation (volume resistivity of about 1015 Ω·cm).
34499468	7	12	dep	insulation	1151:1160	arg1	resistivity					1170:1180	volume resistivity	1163:1180	volume resistivity of about 1015 Ω·cm	1163:1199	The nanocomposite exhibits not only ultrahigh thermal conductivity (in-plane about 5.5 W m-1 K-1 and through-plane about 2.1 W m-1 K-1) but also satisfactory electrical insulation (volume resistivity of about 1015 Ω·cm).
34499468	2	13	from	wood	383:386	arg1	structure					362:370	a naturally aligned microchannel structure	329:370	a naturally aligned microchannel structure from balsa wood	329:386	In this study, a novel strategy to prepare anisotropic wood-based scaffolds with a naturally aligned microchannel structure from balsa wood is demonstrated.
34499468	4	14	theme	wood-polymer	708:719	arg1	nanocomposite					721:733	the wood-polymer nanocomposite	704:733	the wood-polymer nanocomposite	704:733	The highly aligned porous microstructures, with thin wood cell walls and large voids along the cellulose microchannels, allow polymers to enter, resulting in the fabrication of the wood-polymer nanocomposite.
34499468	3	15	theme	small	475:479	arg1	PGO					497:499	PGO	497:499	PGO	497:499	The wood microchannels were coated with polydopamine-surface-modified small graphene oxide (PGO) nanosheets via assembly.
34499468	3	15	theme	small	475:479	arg1	oxide					490:494	small graphene oxide	475:494	polydopamine-surface-modified small graphene oxide (PGO) nanosheets	445:511	The wood microchannels were coated with polydopamine-surface-modified small graphene oxide (PGO) nanosheets via assembly.
34499468	7	16	theme	satisfactory	1127:1138	arg1	insulation					1151:1160	satisfactory electrical insulation	1127:1160	not only ultrahigh thermal conductivity (in-plane about 5.5 W m-1 K-1 and through-plane about 2.1 W m-1 K-1) but also satisfactory electrical insulation (volume resistivity of about 1015 Ω·cm)	1009:1200	The nanocomposite exhibits not only ultrahigh thermal conductivity (in-plane about 5.5 W m-1 K-1 and through-plane about 2.1 W m-1 K-1) but also satisfactory electrical insulation (volume resistivity of about 1015 Ω·cm).
34499468	1	17	theme	advanced	95:102	arg1	composites					112:121	advanced polymer composites	95:121	advanced polymer composites	95:121	Fabricating advanced polymer composites with remarkable mechanical and thermal conductivity performances is desirable for developing advanced devices and equipment.
34499468	7	18	dep	W	1069:1069	arg1	5.5					1065:1067	5.5	1065:1067	5.5	1065:1067	The nanocomposite exhibits not only ultrahigh thermal conductivity (in-plane about 5.5 W m-1 K-1 and through-plane about 2.1 W m-1 K-1) but also satisfactory electrical insulation (volume resistivity of about 1015 Ω·cm).
34499468	1	19	theme	advanced	216:223	arg1	devices					225:231	advanced devices	216:231	advanced devices	216:231	Fabricating advanced polymer composites with remarkable mechanical and thermal conductivity performances is desirable for developing advanced devices and equipment.
34499468	5	20	theme	MJ	854:855	arg1	m-3					857:859	5.0 MJ m-3	850:859	5.0 MJ m-3	850:859	The tensile stiffness and strength of the resulting nanocomposite reach 8.10 GPa and 90.3 MPa with a toughness of 5.0 MJ m-3.
34499468	4	21	theme	porous	546:551	arg1	microstructures					553:567	The highly aligned porous microstructures	527:567	The highly aligned porous microstructures	527:567	The highly aligned porous microstructures, with thin wood cell walls and large voids along the cellulose microchannels, allow polymers to enter, resulting in the fabrication of the wood-polymer nanocomposite.
34499468	1	22	theme	polymer	104:110	arg1	composites					112:121	advanced polymer composites	95:121	advanced polymer composites	95:121	Fabricating advanced polymer composites with remarkable mechanical and thermal conductivity performances is desirable for developing advanced devices and equipment.
34499468	0	23	theme	Wood-Derived	0:11	arg1	Composites					13:22	Wood-Derived Composites	0:22	Wood-Derived Composites with High Performance for Thermal Management Applications	0:80	Wood-Derived Composites with High Performance for Thermal Management Applications.
34499468	8	24	theme	management	1266:1275	arg1	materials					1277:1285	thermal management materials	1258:1285	thermal management materials	1258:1285	Therefore, the results provide a strategy to fabricate thermal management materials with excellent mechanical properties.
34499468	2	25	theme	microchannel	349:360	arg1	structure					362:370	a naturally aligned microchannel structure	329:370	a naturally aligned microchannel structure from balsa wood	329:386	In this study, a novel strategy to prepare anisotropic wood-based scaffolds with a naturally aligned microchannel structure from balsa wood is demonstrated.
34499468	4	26	theme	nanocomposite	721:733	arg1	fabrication					689:699	the fabrication	685:699	the fabrication of the wood-polymer nanocomposite	685:733	The highly aligned porous microstructures, with thin wood cell walls and large voids along the cellulose microchannels, allow polymers to enter, resulting in the fabrication of the wood-polymer nanocomposite.
34499468	0	27	theme	High	29:32	arg1	Performance					34:44	High Performance	29:44	High Performance	29:44	Wood-Derived Composites with High Performance for Thermal Management Applications.
34499468	5	28	theme	m-3	857:859	arg1	toughness					837:845	a toughness	835:845	a toughness of 5.0 MJ m-3	835:859	The tensile stiffness and strength of the resulting nanocomposite reach 8.10 GPa and 90.3 MPa with a toughness of 5.0 MJ m-3.
34499468	7	29	theme	m-1	1109:1111	arg1	K-1					1113:1115	2.1 W m-1 K-1	1103:1115	2.1 W m-1 K-1	1103:1115	The nanocomposite exhibits not only ultrahigh thermal conductivity (in-plane about 5.5 W m-1 K-1 and through-plane about 2.1 W m-1 K-1) but also satisfactory electrical insulation (volume resistivity of about 1015 Ω·cm).
34499468	2	30	theme	aligned	341:347	arg1	structure					362:370	a naturally aligned microchannel structure	329:370	a naturally aligned microchannel structure from balsa wood	329:386	In this study, a novel strategy to prepare anisotropic wood-based scaffolds with a naturally aligned microchannel structure from balsa wood is demonstrated.
34499468	7	31	dep	conductivity	1036:1047	arg1	through-plane					1083:1095	through-plane	1083:1095	through-plane	1083:1095	The nanocomposite exhibits not only ultrahigh thermal conductivity (in-plane about 5.5 W m-1 K-1 and through-plane about 2.1 W m-1 K-1) but also satisfactory electrical insulation (volume resistivity of about 1015 Ω·cm).
34499468	7	31	dep	conductivity	1036:1047	arg1	K-1					1075:1077	in-plane about 5.5 W m-1 K-1	1050:1077	in-plane about 5.5 W m-1 K-1	1050:1077	The nanocomposite exhibits not only ultrahigh thermal conductivity (in-plane about 5.5 W m-1 K-1 and through-plane about 2.1 W m-1 K-1) but also satisfactory electrical insulation (volume resistivity of about 1015 Ω·cm).
34499468	8	32	theme	mechanical	1302:1311	arg1	properties					1313:1322	excellent mechanical properties	1292:1322	excellent mechanical properties	1292:1322	Therefore, the results provide a strategy to fabricate thermal management materials with excellent mechanical properties.
34499468	4	33	theme	cellulose	622:630	arg1	microchannels					632:644	the cellulose microchannels	618:644	the cellulose microchannels	618:644	The highly aligned porous microstructures, with thin wood cell walls and large voids along the cellulose microchannels, allow polymers to enter, resulting in the fabrication of the wood-polymer nanocomposite.
34499468	1	34	theme	remarkable	128:137	arg1	performances					175:186	remarkable mechanical and thermal conductivity performances	128:186	remarkable mechanical and thermal conductivity performances	128:186	Fabricating advanced polymer composites with remarkable mechanical and thermal conductivity performances is desirable for developing advanced devices and equipment.
34499468	8	35	theme	excellent	1292:1300	arg1	properties					1313:1322	excellent mechanical properties	1292:1322	excellent mechanical properties	1292:1322	Therefore, the results provide a strategy to fabricate thermal management materials with excellent mechanical properties.
34499468	3	36	theme	graphene	481:488	arg1	PGO					497:499	PGO	497:499	PGO	497:499	The wood microchannels were coated with polydopamine-surface-modified small graphene oxide (PGO) nanosheets via assembly.
34499468	3	36	theme	graphene	481:488	arg1	oxide					490:494	small graphene oxide	475:494	polydopamine-surface-modified small graphene oxide (PGO) nanosheets	445:511	The wood microchannels were coated with polydopamine-surface-modified small graphene oxide (PGO) nanosheets via assembly.
34499468	2	37	theme	novel	265:269	arg1	strategy					271:278	a novel strategy	263:278	a novel strategy to prepare anisotropic wood-based scaffolds with a naturally aligned microchannel structure from balsa wood	263:386	In this study, a novel strategy to prepare anisotropic wood-based scaffolds with a naturally aligned microchannel structure from balsa wood is demonstrated.
34499468	3	38	theme	oxide	490:494	arg1	nanosheets					502:511	polydopamine-surface-modified small graphene oxide (PGO) nanosheets	445:511	polydopamine-surface-modified small graphene oxide (PGO) nanosheets	445:511	The wood microchannels were coated with polydopamine-surface-modified small graphene oxide (PGO) nanosheets via assembly.
34499468	5	39	theme	resulting	778:786	arg1	nanocomposite					788:800	the resulting nanocomposite	774:800	the resulting nanocomposite	774:800	The tensile stiffness and strength of the resulting nanocomposite reach 8.10 GPa and 90.3 MPa with a toughness of 5.0 MJ m-3.
34499468	7	40	theme	Ω·cm	1196:1199	arg1	resistivity					1170:1180	volume resistivity	1163:1180	volume resistivity of about 1015 Ω·cm	1163:1199	The nanocomposite exhibits not only ultrahigh thermal conductivity (in-plane about 5.5 W m-1 K-1 and through-plane about 2.1 W m-1 K-1) but also satisfactory electrical insulation (volume resistivity of about 1015 Ω·cm).
34499468	0	41	theme	Management	58:67	arg1	Applications					69:80	Thermal Management Applications	50:80	Thermal Management Applications	50:80	Wood-Derived Composites with High Performance for Thermal Management Applications.
34499468	4	42	theme	cell	585:588	arg1	walls					590:594	thin wood cell walls	575:594	thin wood cell walls	575:594	The highly aligned porous microstructures, with thin wood cell walls and large voids along the cellulose microchannels, allow polymers to enter, resulting in the fabrication of the wood-polymer nanocomposite.
34499468	5	43	theme	5.0	850:852	arg1	MJ					854:855	MJ	854:855	MJ	854:855	The tensile stiffness and strength of the resulting nanocomposite reach 8.10 GPa and 90.3 MPa with a toughness of 5.0 MJ m-3.
34499468	6	44	theme	nanocomposite	894:906	arg1	conductivity					874:885	The thermal conductivity	862:885	The thermal conductivity of the nanocomposite	862:906	The thermal conductivity of the nanocomposite is improved significantly by coating a PGO layer onto the wood scaffolds.
34499468	7	45	theme	m-1	1071:1073	arg1	K-1					1075:1077	in-plane about 5.5 W m-1 K-1	1050:1077	in-plane about 5.5 W m-1 K-1	1050:1077	The nanocomposite exhibits not only ultrahigh thermal conductivity (in-plane about 5.5 W m-1 K-1 and through-plane about 2.1 W m-1 K-1) but also satisfactory electrical insulation (volume resistivity of about 1015 Ω·cm).
34499468	1	46	theme	mechanical	139:148	arg1	performances					175:186	remarkable mechanical and thermal conductivity performances	128:186	remarkable mechanical and thermal conductivity performances	128:186	Fabricating advanced polymer composites with remarkable mechanical and thermal conductivity performances is desirable for developing advanced devices and equipment.
34499468	0	47	theme	Thermal	50:56	arg1	Applications					69:80	Thermal Management Applications	50:80	Thermal Management Applications	50:80	Wood-Derived Composites with High Performance for Thermal Management Applications.
34499468	4	48	theme	large	600:604	arg1	voids					606:610	large voids	600:610	large voids	600:610	The highly aligned porous microstructures, with thin wood cell walls and large voids along the cellulose microchannels, allow polymers to enter, resulting in the fabrication of the wood-polymer nanocomposite.
34499468	5	49	theme	tensile	740:746	arg1	stiffness					748:756	The tensile stiffness	736:756	The tensile stiffness	736:756	The tensile stiffness and strength of the resulting nanocomposite reach 8.10 GPa and 90.3 MPa with a toughness of 5.0 MJ m-3.
34499468	0	50	with	Composites	13:22	arg1	Performance					34:44	High Performance	29:44	High Performance	29:44	Wood-Derived Composites with High Performance for Thermal Management Applications.
34499468	2	51	theme	anisotropic	291:301	arg1	scaffolds					314:322	anisotropic wood-based scaffolds	291:322	anisotropic wood-based scaffolds	291:322	In this study, a novel strategy to prepare anisotropic wood-based scaffolds with a naturally aligned microchannel structure from balsa wood is demonstrated.
34499468	1	52	theme	thermal	154:160	arg1	performances					175:186	remarkable mechanical and thermal conductivity performances	128:186	remarkable mechanical and thermal conductivity performances	128:186	Fabricating advanced polymer composites with remarkable mechanical and thermal conductivity performances is desirable for developing advanced devices and equipment.
34499468	2	53	theme	wood-based	303:312	arg1	scaffolds					314:322	anisotropic wood-based scaffolds	291:322	anisotropic wood-based scaffolds	291:322	In this study, a novel strategy to prepare anisotropic wood-based scaffolds with a naturally aligned microchannel structure from balsa wood is demonstrated.
34499468	1	54	theme	conductivity	162:173	arg1	performances					175:186	remarkable mechanical and thermal conductivity performances	128:186	remarkable mechanical and thermal conductivity performances	128:186	Fabricating advanced polymer composites with remarkable mechanical and thermal conductivity performances is desirable for developing advanced devices and equipment.
34499468	2	55	theme	balsa	377:381	arg1	wood					383:386	balsa wood	377:386	balsa wood	377:386	In this study, a novel strategy to prepare anisotropic wood-based scaffolds with a naturally aligned microchannel structure from balsa wood is demonstrated.
34499468	6	56	theme	thermal	866:872	arg1	conductivity					874:885	The thermal conductivity	862:885	The thermal conductivity of the nanocomposite	862:906	The thermal conductivity of the nanocomposite is improved significantly by coating a PGO layer onto the wood scaffolds.
34499468	7	57	theme	in-plane	1050:1057	arg1	K-1					1075:1077	in-plane about 5.5 W m-1 K-1	1050:1077	in-plane about 5.5 W m-1 K-1	1050:1077	The nanocomposite exhibits not only ultrahigh thermal conductivity (in-plane about 5.5 W m-1 K-1 and through-plane about 2.1 W m-1 K-1) but also satisfactory electrical insulation (volume resistivity of about 1015 Ω·cm).
34499468	4	58	with	microstructures	553:567	arg1	walls					590:594	thin wood cell walls	575:594	thin wood cell walls	575:594	The highly aligned porous microstructures, with thin wood cell walls and large voids along the cellulose microchannels, allow polymers to enter, resulting in the fabrication of the wood-polymer nanocomposite.
34499468	4	58	with	microstructures	553:567	arg1	voids					606:610	large voids	600:610	large voids	600:610	The highly aligned porous microstructures, with thin wood cell walls and large voids along the cellulose microchannels, allow polymers to enter, resulting in the fabrication of the wood-polymer nanocomposite.
34499468	2	59	dep	strategy	271:278	arg1	prepare					283:289	prepare	283:289	to prepare anisotropic wood-based scaffolds with a naturally aligned microchannel structure from balsa wood	280:386	In this study, a novel strategy to prepare anisotropic wood-based scaffolds with a naturally aligned microchannel structure from balsa wood is demonstrated.
34499468	3	60	theme	polydopamine-surface-modified	445:473	arg1	nanosheets					502:511	polydopamine-surface-modified small graphene oxide (PGO) nanosheets	445:511	polydopamine-surface-modified small graphene oxide (PGO) nanosheets	445:511	The wood microchannels were coated with polydopamine-surface-modified small graphene oxide (PGO) nanosheets via assembly.
34499468	6	61	theme	wood	966:969	arg1	scaffolds					971:979	the wood scaffolds	962:979	the wood scaffolds	962:979	The thermal conductivity of the nanocomposite is improved significantly by coating a PGO layer onto the wood scaffolds.
34499468	7	62	theme	thermal	1028:1034	arg1	conductivity					1036:1047	ultrahigh thermal conductivity	1018:1047	not only ultrahigh thermal conductivity (in-plane about 5.5 W m-1 K-1 and through-plane about 2.1 W m-1 K-1) but also satisfactory electrical insulation (volume resistivity of about 1015 Ω·cm)	1009:1200	The nanocomposite exhibits not only ultrahigh thermal conductivity (in-plane about 5.5 W m-1 K-1 and through-plane about 2.1 W m-1 K-1) but also satisfactory electrical insulation (volume resistivity of about 1015 Ω·cm).
34048836	0	0	theme	embedded	97:104	arg1	BMP-2					91:95	BMP-2	91:95	BMP-2 embedded in gelatin-elastin-hyaluronic acid hydrogel scaffold for bone tissue engineering application	91:197	Preparation of microfluidic-based pectin microparticles loaded carbon dots conjugated with BMP-2 embedded in gelatin-elastin-hyaluronic acid hydrogel scaffold for bone tissue engineering application.
34048836	1	1	dep	release	350:356	arg1	the					340:342	the	340:342	the	340:342	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability would overcome most of the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration.
34048836	4	2	theme	narrow	919:924	arg1	distribution					931:942	narrow size distribution	919:942	narrow size distribution	919:942	By using microfluidic approach, we encapsulated BMP-2-CDs in pectin microparticles with narrow size distribution and then incorporated into composite scaffolds composed of gelatin, elastin, and hyaluronic acid.
34048836	3	3	theme	loaded	753:758	arg1	microparticles					767:780	BMP-2-CDs loaded pectin microparticles	743:780	BMP-2-CDs loaded pectin microparticles	743:780	With this in mind, the present work aimed to develop a controlled BMP-2-CDs release system using composite scaffolds containing BMP-2-CDs loaded pectin microparticles, which had been optimized for bone regeneration.
34048836	3	4	contain	containing	732:741	arg1	scaffolds					722:730	composite scaffolds	712:730	composite scaffolds containing BMP-2-CDs loaded pectin microparticles	712:780	With this in mind, the present work aimed to develop a controlled BMP-2-CDs release system using composite scaffolds containing BMP-2-CDs loaded pectin microparticles, which had been optimized for bone regeneration.
34048836	3	4	contain	containing	732:741	arg2	microparticles					767:780	BMP-2-CDs loaded pectin microparticles	743:780	BMP-2-CDs loaded pectin microparticles	743:780	With this in mind, the present work aimed to develop a controlled BMP-2-CDs release system using composite scaffolds containing BMP-2-CDs loaded pectin microparticles, which had been optimized for bone regeneration.
34048836	7	5	theme	bioimaging	1428:1437	arg1	agents					1439:1444	promising bioimaging agents	1418:1444	promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds	1418:1518	By utilizing the pectin microparticles carrying BMP-2-CDs as promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds, this platform has immense potential in the field of bone tissue regeneration.
34048836	7	5	theme	bioimaging	1428:1437	arg1	microparticles					1381:1394	the pectin microparticles	1370:1394	the pectin microparticles carrying BMP-2-CDs	1370:1413	By utilizing the pectin microparticles carrying BMP-2-CDs as promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds, this platform has immense potential in the field of bone tissue regeneration.
34048836	2	6	used	used	476:479	arg2	dots					461:464	BMP-2-conjugated carbon dots	437:464	BMP-2-conjugated carbon dots (CDs)	437:470	Herein, BMP-2-conjugated carbon dots (CDs) was used as noninvasive detection platforms to deliver BMP-2 for therapeutic applications where osteogenesis and bioimaging are both required.
34048836	2	6	used	used	476:479	arg2	platforms					506:514	noninvasive detection platforms	484:514	noninvasive detection platforms	484:514	Herein, BMP-2-conjugated carbon dots (CDs) was used as noninvasive detection platforms to deliver BMP-2 for therapeutic applications where osteogenesis and bioimaging are both required.
34048836	2	6	used	used	476:479	arg2	CDs					467:469	CDs	467:469	CDs	467:469	Herein, BMP-2-conjugated carbon dots (CDs) was used as noninvasive detection platforms to deliver BMP-2 for therapeutic applications where osteogenesis and bioimaging are both required.
34048836	1	7	theme	uncontrolled	362:373	arg1	delivery					375:382	uncontrolled delivery	362:382	uncontrolled delivery	362:382	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability would overcome most of the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration.
34048836	5	8	theme	sustained	1103:1111	arg1	fashion					1113:1119	a sustained fashion	1101:1119	a sustained fashion for up to 21 days exhibited a high controlled delivery capacity	1101:1183	The BMP-2-CDs was released from the composite scaffolds in a sustained fashion for up to 21 days exhibited a high controlled delivery capacity.
34048836	5	9	theme	controlled	1156:1165	arg1	capacity					1176:1183	a high controlled delivery capacity	1149:1183	a high controlled delivery capacity	1149:1183	The BMP-2-CDs was released from the composite scaffolds in a sustained fashion for up to 21 days exhibited a high controlled delivery capacity.
34048836	0	10	theme	gelatin-elastin-hyaluronic	109:134	arg1	scaffold					150:157	gelatin-elastin-hyaluronic acid hydrogel scaffold	109:157	gelatin-elastin-hyaluronic acid hydrogel scaffold for bone tissue engineering application	109:197	Preparation of microfluidic-based pectin microparticles loaded carbon dots conjugated with BMP-2 embedded in gelatin-elastin-hyaluronic acid hydrogel scaffold for bone tissue engineering application.
34048836	3	11	theme	composite	712:720	arg1	scaffolds					722:730	composite scaffolds	712:730	composite scaffolds containing BMP-2-CDs loaded pectin microparticles	712:780	With this in mind, the present work aimed to develop a controlled BMP-2-CDs release system using composite scaffolds containing BMP-2-CDs loaded pectin microparticles, which had been optimized for bone regeneration.
34048836	6	12	theme	pro-osteogenic	1334:1347	arg1	effect					1349:1354	pro-osteogenic effect	1334:1354	pro-osteogenic effect	1334:1354	When tested in vitro with MG-63 cells, these extraction mediums showed the intercellular uptake of BMP-2-CDs and enhanced biological properties and pro-osteogenic effect.
34048836	5	13	theme	composite	1078:1086	arg1	scaffolds					1088:1096	the composite scaffolds	1074:1096	the composite scaffolds	1074:1096	The BMP-2-CDs was released from the composite scaffolds in a sustained fashion for up to 21 days exhibited a high controlled delivery capacity.
34048836	7	14	theme	pectin	1374:1379	arg1	agents					1439:1444	promising bioimaging agents	1418:1444	promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds	1418:1518	By utilizing the pectin microparticles carrying BMP-2-CDs as promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds, this platform has immense potential in the field of bone tissue regeneration.
34048836	7	14	theme	pectin	1374:1379	arg1	microparticles					1381:1394	the pectin microparticles	1370:1394	the pectin microparticles carrying BMP-2-CDs	1370:1413	By utilizing the pectin microparticles carrying BMP-2-CDs as promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds, this platform has immense potential in the field of bone tissue regeneration.
34048836	5	15	attach	released	1060:1067	arg2	BMP-2-CDs					1046:1054	The BMP-2-CDs	1042:1054	The BMP-2-CDs	1042:1054	The BMP-2-CDs was released from the composite scaffolds in a sustained fashion for up to 21 days exhibited a high controlled delivery capacity.
34048836	5	15	attach	released	1060:1067	arg1	scaffolds					1088:1096	the composite scaffolds	1074:1096	the composite scaffolds	1074:1096	The BMP-2-CDs was released from the composite scaffolds in a sustained fashion for up to 21 days exhibited a high controlled delivery capacity.
34048836	2	16	theme	therapeutic	537:547	arg1	applications					549:560	therapeutic applications	537:560	therapeutic applications where osteogenesis and bioimaging are both required	537:612	Herein, BMP-2-conjugated carbon dots (CDs) was used as noninvasive detection platforms to deliver BMP-2 for therapeutic applications where osteogenesis and bioimaging are both required.
34048836	3	17	theme	pectin	760:765	arg1	microparticles					767:780	BMP-2-CDs loaded pectin microparticles	743:780	BMP-2-CDs loaded pectin microparticles	743:780	With this in mind, the present work aimed to develop a controlled BMP-2-CDs release system using composite scaffolds containing BMP-2-CDs loaded pectin microparticles, which had been optimized for bone regeneration.
34048836	4	18	theme	size	926:929	arg1	distribution					931:942	narrow size distribution	919:942	narrow size distribution	919:942	By using microfluidic approach, we encapsulated BMP-2-CDs in pectin microparticles with narrow size distribution and then incorporated into composite scaffolds composed of gelatin, elastin, and hyaluronic acid.
34048836	7	19	theme	bone	1573:1576	arg1	regeneration					1585:1596	bone tissue regeneration	1573:1596	bone tissue regeneration	1573:1596	By utilizing the pectin microparticles carrying BMP-2-CDs as promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds, this platform has immense potential in the field of bone tissue regeneration.
34048836	0	20	theme	hydrogel	141:148	arg1	scaffold					150:157	gelatin-elastin-hyaluronic acid hydrogel scaffold	109:157	gelatin-elastin-hyaluronic acid hydrogel scaffold for bone tissue engineering application	109:197	Preparation of microfluidic-based pectin microparticles loaded carbon dots conjugated with BMP-2 embedded in gelatin-elastin-hyaluronic acid hydrogel scaffold for bone tissue engineering application.
34048836	7	21	theme	immense	1539:1545	arg1	potential					1547:1555	immense potential	1539:1555	immense potential	1539:1555	By utilizing the pectin microparticles carrying BMP-2-CDs as promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds, this platform has immense potential in the field of bone tissue regeneration.
34048836	7	22	theme	growth	1450:1455	arg1	factor					1457:1462	growth factor	1450:1462	growth factor delivery	1450:1471	By utilizing the pectin microparticles carrying BMP-2-CDs as promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds, this platform has immense potential in the field of bone tissue regeneration.
34048836	2	23	theme	BMP-2-conjugated	437:452	arg1	CDs					467:469	CDs	467:469	CDs	467:469	Herein, BMP-2-conjugated carbon dots (CDs) was used as noninvasive detection platforms to deliver BMP-2 for therapeutic applications where osteogenesis and bioimaging are both required.
34048836	2	23	theme	BMP-2-conjugated	437:452	arg1	dots					461:464	BMP-2-conjugated carbon dots	437:464	BMP-2-conjugated carbon dots (CDs)	437:470	Herein, BMP-2-conjugated carbon dots (CDs) was used as noninvasive detection platforms to deliver BMP-2 for therapeutic applications where osteogenesis and bioimaging are both required.
34048836	2	23	theme	BMP-2-conjugated	437:452	arg1	platforms					506:514	noninvasive detection platforms	484:514	noninvasive detection platforms	484:514	Herein, BMP-2-conjugated carbon dots (CDs) was used as noninvasive detection platforms to deliver BMP-2 for therapeutic applications where osteogenesis and bioimaging are both required.
34048836	7	24	theme	promising	1418:1426	arg1	agents					1439:1444	promising bioimaging agents	1418:1444	promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds	1418:1518	By utilizing the pectin microparticles carrying BMP-2-CDs as promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds, this platform has immense potential in the field of bone tissue regeneration.
34048836	7	24	theme	promising	1418:1426	arg1	microparticles					1381:1394	the pectin microparticles	1370:1394	the pectin microparticles carrying BMP-2-CDs	1370:1413	By utilizing the pectin microparticles carrying BMP-2-CDs as promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds, this platform has immense potential in the field of bone tissue regeneration.
34048836	1	25	theme	controlled	204:213	arg1	delivery					215:222	The controlled delivery	200:222	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability	200:288	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability would overcome most of the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration.
34048836	0	26	theme	acid	136:139	arg1	scaffold					150:157	gelatin-elastin-hyaluronic acid hydrogel scaffold	109:157	gelatin-elastin-hyaluronic acid hydrogel scaffold for bone tissue engineering application	109:197	Preparation of microfluidic-based pectin microparticles loaded carbon dots conjugated with BMP-2 embedded in gelatin-elastin-hyaluronic acid hydrogel scaffold for bone tissue engineering application.
34048836	1	27	theme	growth	392:397	arg1	factor					399:404	this growth factor	387:404	this growth factor for bone regeneration	387:426	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability would overcome most of the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration.
34048836	6	28	theme	biological	1308:1317	arg1	properties					1319:1328	biological properties	1308:1328	biological properties	1308:1328	When tested in vitro with MG-63 cells, these extraction mediums showed the intercellular uptake of BMP-2-CDs and enhanced biological properties and pro-osteogenic effect.
34048836	6	29	theme	MG-63	1212:1216	arg1	cells					1218:1222	MG-63 cells	1212:1222	MG-63 cells	1212:1222	When tested in vitro with MG-63 cells, these extraction mediums showed the intercellular uptake of BMP-2-CDs and enhanced biological properties and pro-osteogenic effect.
34048836	1	30	theme	factor	399:404	arg1	release					350:356	burst release	344:356	burst release	344:356	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability would overcome most of the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration.
34048836	1	30	theme	factor	399:404	arg1	delivery					375:382	uncontrolled delivery	362:382	uncontrolled delivery	362:382	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability would overcome most of the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration.
34048836	7	31	theme	tissue	1578:1583	arg1	regeneration					1585:1596	bone tissue regeneration	1573:1596	bone tissue regeneration	1573:1596	By utilizing the pectin microparticles carrying BMP-2-CDs as promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds, this platform has immense potential in the field of bone tissue regeneration.
34048836	4	32	theme	pectin	892:897	arg1	microparticles					899:912	pectin microparticles	892:912	pectin microparticles with narrow size distribution	892:942	By using microfluidic approach, we encapsulated BMP-2-CDs in pectin microparticles with narrow size distribution and then incorporated into composite scaffolds composed of gelatin, elastin, and hyaluronic acid.
34048836	1	33	attach	linked	330:335	arg1	release					350:356	burst release	344:356	burst release	344:356	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability would overcome most of the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration.
34048836	1	33	attach	linked	330:335	arg1	delivery					375:382	uncontrolled delivery	362:382	uncontrolled delivery	362:382	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability would overcome most of the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration.
34048836	1	33	attach	linked	330:335	arg2	effects					322:328	the side effects	313:328	the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration	313:426	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability would overcome most of the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration.
34048836	0	34	theme	pectin	34:39	arg1	microparticles					41:54	microfluidic-based pectin microparticles	15:54	microfluidic-based pectin microparticles	15:54	Preparation of microfluidic-based pectin microparticles loaded carbon dots conjugated with BMP-2 embedded in gelatin-elastin-hyaluronic acid hydrogel scaffold for bone tissue engineering application.
34048836	7	35	contain	carrying	1396:1403	arg1	agents					1439:1444	promising bioimaging agents	1418:1444	promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds	1418:1518	By utilizing the pectin microparticles carrying BMP-2-CDs as promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds, this platform has immense potential in the field of bone tissue regeneration.
34048836	7	35	contain	carrying	1396:1403	arg2	BMP-2-CDs					1405:1413	BMP-2-CDs	1405:1413	BMP-2-CDs	1405:1413	By utilizing the pectin microparticles carrying BMP-2-CDs as promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds, this platform has immense potential in the field of bone tissue regeneration.
34048836	7	35	contain	carrying	1396:1403	arg1	microparticles					1381:1394	the pectin microparticles	1370:1394	the pectin microparticles carrying BMP-2-CDs	1370:1413	By utilizing the pectin microparticles carrying BMP-2-CDs as promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds, this platform has immense potential in the field of bone tissue regeneration.
34048836	7	36	theme	factor	1457:1462	arg1	delivery					1464:1471	growth factor delivery	1450:1471	growth factor delivery	1450:1471	By utilizing the pectin microparticles carrying BMP-2-CDs as promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds, this platform has immense potential in the field of bone tissue regeneration.
34048836	1	37	theme	bone	231:234	arg1	BMP-2					261:265	BMP-2	261:265	BMP-2	261:265	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability would overcome most of the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration.
34048836	1	37	theme	bone	231:234	arg1	protein-2					250:258	bone morphogenetic protein-2	231:258	the bone morphogenetic protein-2 (BMP-2)	227:266	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability would overcome most of the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration.
34048836	0	38	theme	microfluidic-based	15:32	arg1	microparticles					41:54	microfluidic-based pectin microparticles	15:54	microfluidic-based pectin microparticles	15:54	Preparation of microfluidic-based pectin microparticles loaded carbon dots conjugated with BMP-2 embedded in gelatin-elastin-hyaluronic acid hydrogel scaffold for bone tissue engineering application.
34048836	1	39	theme	side	317:320	arg1	effects					322:328	the side effects	313:328	the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration	313:426	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability would overcome most of the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration.
34048836	4	40	theme	composite	971:979	arg1	scaffolds					981:989	composite scaffolds	971:989	composite scaffolds composed of gelatin, elastin, and hyaluronic acid	971:1039	By using microfluidic approach, we encapsulated BMP-2-CDs in pectin microparticles with narrow size distribution and then incorporated into composite scaffolds composed of gelatin, elastin, and hyaluronic acid.
34048836	0	41	from	embedded	97:104	arg1	scaffold					150:157	gelatin-elastin-hyaluronic acid hydrogel scaffold	109:157	gelatin-elastin-hyaluronic acid hydrogel scaffold for bone tissue engineering application	109:197	Preparation of microfluidic-based pectin microparticles loaded carbon dots conjugated with BMP-2 embedded in gelatin-elastin-hyaluronic acid hydrogel scaffold for bone tissue engineering application.
34048836	1	42	theme	morphogenetic	236:248	arg1	BMP-2					261:265	BMP-2	261:265	BMP-2	261:265	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability would overcome most of the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration.
34048836	1	42	theme	morphogenetic	236:248	arg1	protein-2					250:258	bone morphogenetic protein-2	231:258	the bone morphogenetic protein-2 (BMP-2)	227:266	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability would overcome most of the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration.
34048836	2	43	theme	noninvasive	484:494	arg1	dots					461:464	BMP-2-conjugated carbon dots	437:464	BMP-2-conjugated carbon dots (CDs)	437:470	Herein, BMP-2-conjugated carbon dots (CDs) was used as noninvasive detection platforms to deliver BMP-2 for therapeutic applications where osteogenesis and bioimaging are both required.
34048836	2	43	theme	noninvasive	484:494	arg1	platforms					506:514	noninvasive detection platforms	484:514	noninvasive detection platforms	484:514	Herein, BMP-2-conjugated carbon dots (CDs) was used as noninvasive detection platforms to deliver BMP-2 for therapeutic applications where osteogenesis and bioimaging are both required.
34048836	0	44	theme	tissue	168:173	arg1	engineering					175:185	bone tissue engineering	163:185	bone tissue engineering application	163:197	Preparation of microfluidic-based pectin microparticles loaded carbon dots conjugated with BMP-2 embedded in gelatin-elastin-hyaluronic acid hydrogel scaffold for bone tissue engineering application.
34048836	1	45	theme	bone	410:413	arg1	regeneration					415:426	bone regeneration	410:426	bone regeneration	410:426	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability would overcome most of the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration.
34048836	6	46	theme	BMP-2-CDs	1285:1293	arg1	uptake					1275:1280	the intercellular uptake	1257:1280	the intercellular uptake of BMP-2-CDs	1257:1293	When tested in vitro with MG-63 cells, these extraction mediums showed the intercellular uptake of BMP-2-CDs and enhanced biological properties and pro-osteogenic effect.
34048836	2	47	theme	detection	496:504	arg1	dots					461:464	BMP-2-conjugated carbon dots	437:464	BMP-2-conjugated carbon dots (CDs)	437:470	Herein, BMP-2-conjugated carbon dots (CDs) was used as noninvasive detection platforms to deliver BMP-2 for therapeutic applications where osteogenesis and bioimaging are both required.
34048836	2	47	theme	detection	496:504	arg1	platforms					506:514	noninvasive detection platforms	484:514	noninvasive detection platforms	484:514	Herein, BMP-2-conjugated carbon dots (CDs) was used as noninvasive detection platforms to deliver BMP-2 for therapeutic applications where osteogenesis and bioimaging are both required.
34048836	1	48	theme	protein-2	250:258	arg1	delivery					215:222	The controlled delivery	200:222	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability	200:288	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability would overcome most of the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration.
34048836	0	49	theme	microparticles	41:54	arg1	Preparation					0:10	Preparation	0:10	Preparation of microfluidic-based pectin microparticles	0:54	Preparation of microfluidic-based pectin microparticles loaded carbon dots conjugated with BMP-2 embedded in gelatin-elastin-hyaluronic acid hydrogel scaffold for bone tissue engineering application.
34048836	3	50	theme	bone	812:815	arg1	regeneration					817:828	bone regeneration	812:828	bone regeneration	812:828	With this in mind, the present work aimed to develop a controlled BMP-2-CDs release system using composite scaffolds containing BMP-2-CDs loaded pectin microparticles, which had been optimized for bone regeneration.
34048836	0	51	theme	bone	163:166	arg1	engineering					175:185	bone tissue engineering	163:185	bone tissue engineering application	163:197	Preparation of microfluidic-based pectin microparticles loaded carbon dots conjugated with BMP-2 embedded in gelatin-elastin-hyaluronic acid hydrogel scaffold for bone tissue engineering application.
34048836	2	52	dep	required	605:612	arg1	both					600:603	both	600:603	both	600:603	Herein, BMP-2-conjugated carbon dots (CDs) was used as noninvasive detection platforms to deliver BMP-2 for therapeutic applications where osteogenesis and bioimaging are both required.
34048836	5	53	theme	high	1151:1154	arg1	capacity					1176:1183	a high controlled delivery capacity	1149:1183	a high controlled delivery capacity	1149:1183	The BMP-2-CDs was released from the composite scaffolds in a sustained fashion for up to 21 days exhibited a high controlled delivery capacity.
34048836	0	54	theme	carbon	63:68	arg1	dots					70:73	carbon dots	63:73	carbon dots	63:73	Preparation of microfluidic-based pectin microparticles loaded carbon dots conjugated with BMP-2 embedded in gelatin-elastin-hyaluronic acid hydrogel scaffold for bone tissue engineering application.
34048836	0	55	theme	engineering	175:185	arg1	application					187:197	bone tissue engineering application	163:197	bone tissue engineering application	163:197	Preparation of microfluidic-based pectin microparticles loaded carbon dots conjugated with BMP-2 embedded in gelatin-elastin-hyaluronic acid hydrogel scaffold for bone tissue engineering application.
34048836	3	56	theme	release	691:697	arg1	system					699:704	a controlled BMP-2-CDs release system	668:704	a controlled BMP-2-CDs release system using composite scaffolds containing BMP-2-CDs loaded pectin microparticles	668:780	With this in mind, the present work aimed to develop a controlled BMP-2-CDs release system using composite scaffolds containing BMP-2-CDs loaded pectin microparticles, which had been optimized for bone regeneration.
34048836	6	57	theme	intercellular	1261:1273	arg1	uptake					1275:1280	the intercellular uptake	1257:1280	the intercellular uptake of BMP-2-CDs	1257:1293	When tested in vitro with MG-63 cells, these extraction mediums showed the intercellular uptake of BMP-2-CDs and enhanced biological properties and pro-osteogenic effect.
34048836	1	58	theme	burst	344:348	arg1	release					350:356	burst release	344:356	burst release	344:356	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability would overcome most of the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration.
34048836	7	59	theme	regeneration	1585:1596	arg1	field					1564:1568	the field	1560:1568	the field of bone tissue regeneration	1560:1596	By utilizing the pectin microparticles carrying BMP-2-CDs as promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds, this platform has immense potential in the field of bone tissue regeneration.
34048836	3	60	theme	present	638:644	arg1	work					646:649	the present work	634:649	the present work	634:649	With this in mind, the present work aimed to develop a controlled BMP-2-CDs release system using composite scaffolds containing BMP-2-CDs loaded pectin microparticles, which had been optimized for bone regeneration.
34048836	1	61	with	delivery	215:222	arg1	ability					282:288	tracking ability	273:288	tracking ability	273:288	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability would overcome most of the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration.
34048836	3	62	theme	controlled	670:679	arg1	system					699:704	a controlled BMP-2-CDs release system	668:704	a controlled BMP-2-CDs release system using composite scaffolds containing BMP-2-CDs loaded pectin microparticles	668:780	With this in mind, the present work aimed to develop a controlled BMP-2-CDs release system using composite scaffolds containing BMP-2-CDs loaded pectin microparticles, which had been optimized for bone regeneration.
34048836	7	63	theme	scaffolds	1510:1518	arg1	composition					1491:1501	the composition	1487:1501	the composition of the scaffolds	1487:1518	By utilizing the pectin microparticles carrying BMP-2-CDs as promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds, this platform has immense potential in the field of bone tissue regeneration.
34048836	7	63	theme	scaffolds	1510:1518	arg1	tuning					1480:1485	tuning	1480:1485	tuning	1480:1485	By utilizing the pectin microparticles carrying BMP-2-CDs as promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds, this platform has immense potential in the field of bone tissue regeneration.
34048836	7	64	contain	has	1535:1537	arg1	platform					1526:1533	this platform	1521:1533	this platform	1521:1533	By utilizing the pectin microparticles carrying BMP-2-CDs as promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds, this platform has immense potential in the field of bone tissue regeneration.
34048836	7	64	contain	has	1535:1537	arg2	potential					1547:1555	immense potential	1539:1555	immense potential	1539:1555	By utilizing the pectin microparticles carrying BMP-2-CDs as promising bioimaging agents for growth factor delivery and by tuning the composition of the scaffolds, this platform has immense potential in the field of bone tissue regeneration.
34048836	6	65	theme	extraction	1231:1240	arg1	mediums					1242:1248	these extraction mediums	1225:1248	these extraction mediums	1225:1248	When tested in vitro with MG-63 cells, these extraction mediums showed the intercellular uptake of BMP-2-CDs and enhanced biological properties and pro-osteogenic effect.
34048836	4	66	theme	hyaluronic	1025:1034	arg1	acid					1036:1039	hyaluronic acid	1025:1039	hyaluronic acid	1025:1039	By using microfluidic approach, we encapsulated BMP-2-CDs in pectin microparticles with narrow size distribution and then incorporated into composite scaffolds composed of gelatin, elastin, and hyaluronic acid.
34048836	3	67	theme	BMP-2-CDs	681:689	arg1	system					699:704	a controlled BMP-2-CDs release system	668:704	a controlled BMP-2-CDs release system using composite scaffolds containing BMP-2-CDs loaded pectin microparticles	668:780	With this in mind, the present work aimed to develop a controlled BMP-2-CDs release system using composite scaffolds containing BMP-2-CDs loaded pectin microparticles, which had been optimized for bone regeneration.
34048836	4	68	theme	microfluidic	840:851	arg1	approach					853:860	microfluidic approach	840:860	microfluidic approach	840:860	By using microfluidic approach, we encapsulated BMP-2-CDs in pectin microparticles with narrow size distribution and then incorporated into composite scaffolds composed of gelatin, elastin, and hyaluronic acid.
34048836	2	69	theme	carbon	454:459	arg1	CDs					467:469	CDs	467:469	CDs	467:469	Herein, BMP-2-conjugated carbon dots (CDs) was used as noninvasive detection platforms to deliver BMP-2 for therapeutic applications where osteogenesis and bioimaging are both required.
34048836	2	69	theme	carbon	454:459	arg1	dots					461:464	BMP-2-conjugated carbon dots	437:464	BMP-2-conjugated carbon dots (CDs)	437:470	Herein, BMP-2-conjugated carbon dots (CDs) was used as noninvasive detection platforms to deliver BMP-2 for therapeutic applications where osteogenesis and bioimaging are both required.
34048836	2	69	theme	carbon	454:459	arg1	platforms					506:514	noninvasive detection platforms	484:514	noninvasive detection platforms	484:514	Herein, BMP-2-conjugated carbon dots (CDs) was used as noninvasive detection platforms to deliver BMP-2 for therapeutic applications where osteogenesis and bioimaging are both required.
34048836	5	70	theme	delivery	1167:1174	arg1	capacity					1176:1183	a high controlled delivery capacity	1149:1183	a high controlled delivery capacity	1149:1183	The BMP-2-CDs was released from the composite scaffolds in a sustained fashion for up to 21 days exhibited a high controlled delivery capacity.
34048836	0	71	from	scaffold	150:157	arg1	embedded					97:104	embedded	97:104	embedded	97:104	Preparation of microfluidic-based pectin microparticles loaded carbon dots conjugated with BMP-2 embedded in gelatin-elastin-hyaluronic acid hydrogel scaffold for bone tissue engineering application.
34048836	4	72	with	microparticles	899:912	arg1	distribution					931:942	narrow size distribution	919:942	narrow size distribution	919:942	By using microfluidic approach, we encapsulated BMP-2-CDs in pectin microparticles with narrow size distribution and then incorporated into composite scaffolds composed of gelatin, elastin, and hyaluronic acid.
34048836	1	73	theme	tracking	273:280	arg1	ability					282:288	tracking ability	273:288	tracking ability	273:288	The controlled delivery of the bone morphogenetic protein-2 (BMP-2) with tracking ability would overcome most of the side effects linked to the burst release and uncontrolled delivery of this growth factor for bone regeneration.
33142597	3	0	theme	X-ray	487:491	arg1	diffraction					493:503	X-ray diffraction	487:503	X-ray diffraction (XRD)	487:509	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	3	0	theme	X-ray	487:491	arg1	XRD					506:508	XRD	506:508	XRD	506:508	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	3	1	theme	vapor	638:642	arg1	WVP					658:660	WVP	658:660	WVP	658:660	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	3	1	theme	vapor	638:642	arg1	permeability					644:655	water vapor permeability	632:655	water vapor permeability (WVP)	632:661	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	6	2	from	break	937:941	arg1	at					934:935	elongation at break	923:941	elongation at break	923:941	The tensile strength, elongation at break and water resistance of HCS film were also improved significantly.
33142597	6	2	from	break	937:941	arg1	elongation					923:932	elongation at break	923:941	elongation at break	923:941	The tensile strength, elongation at break and water resistance of HCS film were also improved significantly.
33142597	6	3	theme	at	934:935	arg1	break					937:941	elongation at break	923:941	elongation at break	923:941	The tensile strength, elongation at break and water resistance of HCS film were also improved significantly.
33142597	0	4	theme	barrier	101:107	arg1	properties					109:118	improved mechanical and barrier properties	77:118	properties	109:118	Formation of high amylose corn starch/konjac glucomannan composite film with improved mechanical and barrier properties.
33142597	4	5	theme	order	716:720	arg1	structure					722:730	short-range order structure	704:730	short-range order structure of the films	704:743	The crystallinity and the proportion of short-range order structure of the films increased first and then declined with the addition of KGM.
33142597	6	6	theme	elongation	923:932	arg1	break					937:941	elongation at break	923:941	elongation at break	923:941	The tensile strength, elongation at break and water resistance of HCS film were also improved significantly.
33142597	3	7	from	influence	352:360	arg1	properties					389:398	the film-forming properties	372:398	the film-forming properties of HCS	372:405	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	3	7	from	influence	352:360	arg1	properties					431:440	the physicochemical properties	411:440	the physicochemical properties of the resulting films	411:463	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	4	8	theme	short-range	704:714	arg1	structure					722:730	short-range order structure	704:730	short-range order structure of the films	704:743	The crystallinity and the proportion of short-range order structure of the films increased first and then declined with the addition of KGM.
33142597	4	9	theme	films	739:743	arg1	structure					722:730	short-range order structure	704:730	short-range order structure of the films	704:743	The crystallinity and the proportion of short-range order structure of the films increased first and then declined with the addition of KGM.
33142597	8	10	theme	addition	1107:1114	arg1	KGM					1126:1128	KGM	1126:1128	KGM	1126:1128	The optimal addition amount of KGM was 0.3 %.
33142597	8	10	theme	addition	1107:1114	arg1	%					1138:1138	0.3 %	1134:1138	0.3 %	1134:1138	The optimal addition amount of KGM was 0.3 %.
33142597	8	10	theme	addition	1107:1114	arg1	amount					1116:1121	The optimal addition amount	1095:1121	The optimal addition amount of KGM	1095:1128	The optimal addition amount of KGM was 0.3 %.
33142597	0	11	with	film	67:70	arg1	mechanical					86:95	mechanical	86:95	mechanical	86:95	Formation of high amylose corn starch/konjac glucomannan composite film with improved mechanical and barrier properties.
33142597	0	11	with	film	67:70	arg1	properties					109:118	improved mechanical and barrier properties	77:118	properties	109:118	Formation of high amylose corn starch/konjac glucomannan composite film with improved mechanical and barrier properties.
33142597	2	12	theme	corn	285:288	arg1	starch					290:295	high amylose corn starch	272:295	high amylose corn starch (HCS)	272:301	In the present study, glycerol-plasticized composite films based on high amylose corn starch (HCS) and konjac glucomannan (KGM) were developed.
33142597	2	12	theme	corn	285:288	arg1	HCS					298:300	HCS	298:300	HCS	298:300	In the present study, glycerol-plasticized composite films based on high amylose corn starch (HCS) and konjac glucomannan (KGM) were developed.
33142597	3	13	theme	HCS	403:405	arg1	properties					389:398	the film-forming properties	372:398	the film-forming properties of HCS	372:405	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	3	13	theme	HCS	403:405	arg1	properties					431:440	the physicochemical properties	411:440	the physicochemical properties of the resulting films	411:463	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	8	14	theme	optimal	1099:1105	arg1	KGM					1126:1128	KGM	1126:1128	KGM	1126:1128	The optimal addition amount of KGM was 0.3 %.
33142597	8	14	theme	optimal	1099:1105	arg1	%					1138:1138	0.3 %	1134:1138	0.3 %	1134:1138	The optimal addition amount of KGM was 0.3 %.
33142597	8	14	theme	optimal	1099:1105	arg1	amount					1116:1121	The optimal addition amount	1095:1121	The optimal addition amount of KGM	1095:1128	The optimal addition amount of KGM was 0.3 %.
33142597	2	15	theme	amylose	277:283	arg1	starch					290:295	high amylose corn starch	272:295	high amylose corn starch (HCS)	272:301	In the present study, glycerol-plasticized composite films based on high amylose corn starch (HCS) and konjac glucomannan (KGM) were developed.
33142597	2	15	theme	amylose	277:283	arg1	HCS					298:300	HCS	298:300	HCS	298:300	In the present study, glycerol-plasticized composite films based on high amylose corn starch (HCS) and konjac glucomannan (KGM) were developed.
33142597	1	16	theme	research	183:190	arg1	points					196:201	research hot points	183:201	research hot points	183:201	Recently, starch-based packaging materials have become one of research hot points.
33142597	3	17	theme	KGM	365:367	arg1	influence					352:360	The influence	348:360	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films	348:463	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	4	18	theme	KGM	800:802	arg1	addition					788:795	the addition	784:795	the addition of KGM	784:802	The crystallinity and the proportion of short-range order structure of the films increased first and then declined with the addition of KGM.
33142597	2	19	theme	konjac	307:312	arg1	KGM					327:329	KGM	327:329	KGM	327:329	In the present study, glycerol-plasticized composite films based on high amylose corn starch (HCS) and konjac glucomannan (KGM) were developed.
33142597	2	19	theme	konjac	307:312	arg1	glucomannan					314:324	konjac glucomannan	307:324	konjac glucomannan (KGM)	307:330	In the present study, glycerol-plasticized composite films based on high amylose corn starch (HCS) and konjac glucomannan (KGM) were developed.
33142597	1	20	theme	hot	192:194	arg1	points					196:201	research hot points	183:201	research hot points	183:201	Recently, starch-based packaging materials have become one of research hot points.
33142597	7	21	theme	synergistic	1014:1024	arg1	effect					1026:1031	The synergistic effect	1010:1031	The synergistic effect between HCS and KGM	1010:1051	The synergistic effect between HCS and KGM improved the film-forming ability of HCS.
33142597	1	22	theme	points	196:201	arg1	points					196:201	research hot points	183:201	research hot points	183:201	Recently, starch-based packaging materials have become one of research hot points.
33142597	1	22	theme	points	196:201	arg1	one					176:178	one	176:178	one	176:178	Recently, starch-based packaging materials have become one of research hot points.
33142597	0	23	theme	amylose	18:24	arg1	starch/konjac					31:43	high amylose corn starch/konjac	13:43	high amylose corn starch/konjac	13:43	Formation of high amylose corn starch/konjac glucomannan composite film with improved mechanical and barrier properties.
33142597	6	24	theme	tensile	905:911	arg1	strength					913:920	The tensile strength	901:920	The tensile strength	901:920	The tensile strength, elongation at break and water resistance of HCS film were also improved significantly.
33142597	3	25	theme	infrared	530:537	arg1	spectroscopy					539:550	infrared spectroscopy	530:550	infrared spectroscopy (FTIR)	530:557	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	3	25	theme	infrared	530:537	arg1	FTIR					553:556	FTIR	553:556	FTIR	553:556	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	3	26	theme	scanning	560:567	arg1	SEM					590:592	SEM	590:592	SEM	590:592	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	3	26	theme	scanning	560:567	arg1	microscopy					578:587	scanning electron microscopy	560:587	scanning electron microscopy (SEM)	560:593	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	0	27	theme	high	13:16	arg1	starch/konjac					31:43	high amylose corn starch/konjac	13:43	high amylose corn starch/konjac	13:43	Formation of high amylose corn starch/konjac glucomannan composite film with improved mechanical and barrier properties.
33142597	3	28	dep	Fourier	512:518	arg1	transform					520:528	transform	520:528	transform infrared spectroscopy (FTIR)	520:557	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	3	29	theme	resulting	449:457	arg1	films					459:463	the resulting films	445:463	the resulting films	445:463	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	0	30	theme	starch/konjac	31:43	arg1	Formation					0:8	Formation	0:8	Formation of high amylose corn starch/konjac	0:43	Formation of high amylose corn starch/konjac glucomannan composite film with improved mechanical and barrier properties.
33142597	2	31	theme	composite	247:255	arg1	films					257:261	glycerol-plasticized composite films	226:261	glycerol-plasticized composite films based on high amylose corn starch (HCS) and konjac glucomannan (KGM)	226:330	In the present study, glycerol-plasticized composite films based on high amylose corn starch (HCS) and konjac glucomannan (KGM) were developed.
33142597	6	32	theme	film	971:974	arg1	break					937:941	elongation at break	923:941	elongation at break	923:941	The tensile strength, elongation at break and water resistance of HCS film were also improved significantly.
33142597	6	32	theme	film	971:974	arg1	resistance					953:962	water resistance	947:962	water resistance	947:962	The tensile strength, elongation at break and water resistance of HCS film were also improved significantly.
33142597	6	32	theme	film	971:974	arg1	strength					913:920	The tensile strength	901:920	The tensile strength	901:920	The tensile strength, elongation at break and water resistance of HCS film were also improved significantly.
33142597	0	33	theme	corn	26:29	arg1	starch/konjac					31:43	high amylose corn starch/konjac	13:43	high amylose corn starch/konjac	13:43	Formation of high amylose corn starch/konjac glucomannan composite film with improved mechanical and barrier properties.
33142597	7	34	theme	HCS	1090:1092	arg1	ability					1079:1085	the film-forming ability	1062:1085	the film-forming ability of HCS	1062:1092	The synergistic effect between HCS and KGM improved the film-forming ability of HCS.
33142597	2	35	theme	glycerol-plasticized	226:245	arg1	films					257:261	glycerol-plasticized composite films	226:261	glycerol-plasticized composite films based on high amylose corn starch (HCS) and konjac glucomannan (KGM)	226:330	In the present study, glycerol-plasticized composite films based on high amylose corn starch (HCS) and konjac glucomannan (KGM) were developed.
33142597	6	36	theme	HCS	967:969	arg1	film					971:974	HCS film	967:974	HCS film	967:974	The tensile strength, elongation at break and water resistance of HCS film were also improved significantly.
33142597	0	37	theme	composite	57:65	arg1	film					67:70	composite film	57:70	composite film with improved mechanical and barrier properties	57:118	Formation of high amylose corn starch/konjac glucomannan composite film with improved mechanical and barrier properties.
33142597	3	38	theme	thermogravimetric	596:612	arg1	analysis					619:626	thermogravimetric (TG) analysis	596:626	thermogravimetric (TG) analysis	596:626	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	3	38	theme	thermogravimetric	596:612	arg1	TG					615:616	TG	615:616	TG	615:616	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	3	39	theme	film-forming	376:387	arg1	properties					389:398	the film-forming properties	372:398	the film-forming properties of HCS	372:405	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	2	40	theme	present	211:217	arg1	study					219:223	the present study	207:223	the present study	207:223	In the present study, glycerol-plasticized composite films based on high amylose corn starch (HCS) and konjac glucomannan (KGM) were developed.
33142597	5	41	theme	even	857:860	arg1	texture					862:868	the more even texture	848:868	the more even texture	848:868	The micromorphology of the films exhibited the more even texture after KGM was incorporated in.
33142597	3	42	theme	water	632:636	arg1	WVP					658:660	WVP	658:660	WVP	658:660	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	3	42	theme	water	632:636	arg1	permeability					644:655	water vapor permeability	632:655	water vapor permeability (WVP)	632:661	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	2	43	theme	high	272:275	arg1	starch					290:295	high amylose corn starch	272:295	high amylose corn starch (HCS)	272:301	In the present study, glycerol-plasticized composite films based on high amylose corn starch (HCS) and konjac glucomannan (KGM) were developed.
33142597	2	43	theme	high	272:275	arg1	HCS					298:300	HCS	298:300	HCS	298:300	In the present study, glycerol-plasticized composite films based on high amylose corn starch (HCS) and konjac glucomannan (KGM) were developed.
33142597	3	44	theme	electron	569:576	arg1	SEM					590:592	SEM	590:592	SEM	590:592	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	3	44	theme	electron	569:576	arg1	microscopy					578:587	scanning electron microscopy	560:587	scanning electron microscopy (SEM)	560:593	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	1	45	theme	starch-based	131:142	arg1	materials					154:162	starch-based packaging materials	131:162	starch-based packaging materials	131:162	Recently, starch-based packaging materials have become one of research hot points.
33142597	4	46	theme	structure	722:730	arg1	proportion					690:699	the proportion	686:699	the proportion of short-range order structure of the films	686:743	The crystallinity and the proportion of short-range order structure of the films increased first and then declined with the addition of KGM.
33142597	4	46	theme	structure	722:730	arg1	crystallinity					668:680	The crystallinity	664:680	The crystallinity	664:680	The crystallinity and the proportion of short-range order structure of the films increased first and then declined with the addition of KGM.
33142597	1	47	theme	packaging	144:152	arg1	materials					154:162	starch-based packaging materials	131:162	starch-based packaging materials	131:162	Recently, starch-based packaging materials have become one of research hot points.
33142597	3	48	theme	films	459:463	arg1	properties					389:398	the film-forming properties	372:398	the film-forming properties of HCS	372:405	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	3	48	theme	films	459:463	arg1	properties					431:440	the physicochemical properties	411:440	the physicochemical properties of the resulting films	411:463	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	3	49	theme	physicochemical	415:429	arg1	properties					431:440	the physicochemical properties	411:440	the physicochemical properties of the resulting films	411:463	The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP).
33142597	6	50	theme	water	947:951	arg1	resistance					953:962	water resistance	947:962	water resistance	947:962	The tensile strength, elongation at break and water resistance of HCS film were also improved significantly.
33142597	7	51	theme	film-forming	1066:1077	arg1	ability					1079:1085	the film-forming ability	1062:1085	the film-forming ability of HCS	1062:1092	The synergistic effect between HCS and KGM improved the film-forming ability of HCS.
33142597	8	52	theme	KGM	1126:1128	arg1	KGM					1126:1128	KGM	1126:1128	KGM	1126:1128	The optimal addition amount of KGM was 0.3 %.
33142597	8	52	theme	KGM	1126:1128	arg1	%					1138:1138	0.3 %	1134:1138	0.3 %	1134:1138	The optimal addition amount of KGM was 0.3 %.
33142597	8	52	theme	KGM	1126:1128	arg1	amount					1116:1121	The optimal addition amount	1095:1121	The optimal addition amount of KGM	1095:1128	The optimal addition amount of KGM was 0.3 %.
33142597	5	53	theme	films	832:836	arg1	micromorphology					809:823	The micromorphology	805:823	The micromorphology of the films	805:836	The micromorphology of the films exhibited the more even texture after KGM was incorporated in.
33652598	8	0	from	growth	1133:1138	arg1	surface					1147:1153	its surface	1143:1153	its surface	1143:1153	The granulate has been proven in vitro to be non-cytotoxic, suitable for MG63 cell growth on its surface, and increasing alkaline phosphatase activity, an important biological marker of bone tissue growth.
33652598	9	1	theme	bone	1471:1474	arg1	tissue					1476:1481	bone tissue	1471:1481	bone tissue	1471:1481	Moreover, the granulate is suitable for thermal sterilization without losing its form-increasing its convenience for application in surgery for guided bone regeneration in case of minor or non-load bearing voids in bone tissue.
33652598	9	2	theme	non-load	1445:1452	arg1	voids					1462:1466	minor or non-load bearing voids	1436:1466	minor or non-load bearing voids in bone tissue	1436:1481	Moreover, the granulate is suitable for thermal sterilization without losing its form-increasing its convenience for application in surgery for guided bone regeneration in case of minor or non-load bearing voids in bone tissue.
33652598	8	3	theme	growth	1248:1253	arg1	marker					1226:1231	an important biological marker	1202:1231	an important biological marker of bone tissue growth	1202:1253	The granulate has been proven in vitro to be non-cytotoxic, suitable for MG63 cell growth on its surface, and increasing alkaline phosphatase activity, an important biological marker of bone tissue growth.
33652598	8	3	theme	growth	1248:1253	arg1	activity					1192:1199	alkaline phosphatase activity	1171:1199	alkaline phosphatase activity	1171:1199	The granulate has been proven in vitro to be non-cytotoxic, suitable for MG63 cell growth on its surface, and increasing alkaline phosphatase activity, an important biological marker of bone tissue growth.
33652598	9	4	theme	bearing	1454:1460	arg1	voids					1462:1466	minor or non-load bearing voids	1436:1466	minor or non-load bearing voids in bone tissue	1436:1481	Moreover, the granulate is suitable for thermal sterilization without losing its form-increasing its convenience for application in surgery for guided bone regeneration in case of minor or non-load bearing voids in bone tissue.
33652598	9	5	theme	minor	1436:1440	arg1	voids					1462:1466	minor or non-load bearing voids	1436:1466	minor or non-load bearing voids in bone tissue	1436:1481	Moreover, the granulate is suitable for thermal sterilization without losing its form-increasing its convenience for application in surgery for guided bone regeneration in case of minor or non-load bearing voids in bone tissue.
33652598	6	6	theme	potent	842:847	arg1	biopolymer					849:858	chitosan-a potent biopolymer	831:858	chitosan-a potent biopolymer	831:858	A granulate composed of β-tricalcium phosphate, pulverized human bone, and chitosan-a potent biopolymer applied in tissue engineering, regenerative medicine, and biotechnology-has been developed.
33652598	9	7	from	voids	1462:1466	arg1	tissue					1476:1481	bone tissue	1471:1481	bone tissue	1471:1481	Moreover, the granulate is suitable for thermal sterilization without losing its form-increasing its convenience for application in surgery for guided bone regeneration in case of minor or non-load bearing voids in bone tissue.
33652598	6	8	theme	chitosan-a	831:840	arg1	biopolymer					849:858	chitosan-a potent biopolymer	831:858	chitosan-a potent biopolymer	831:858	A granulate composed of β-tricalcium phosphate, pulverized human bone, and chitosan-a potent biopolymer applied in tissue engineering, regenerative medicine, and biotechnology-has been developed.
33652598	1	9	from	topic	134:138	arg1	medicine					164:171	medicine	164:171	medicine	164:171	The search for the perfect bone graft material is an important topic in material science and medicine.
33652598	1	9	from	topic	134:138	arg1	science					152:158	science	152:158	science	152:158	The search for the perfect bone graft material is an important topic in material science and medicine.
33652598	8	10	theme	cell	1128:1131	arg1	growth					1133:1138	MG63 cell growth	1123:1138	MG63 cell growth on its surface	1123:1153	The granulate has been proven in vitro to be non-cytotoxic, suitable for MG63 cell growth on its surface, and increasing alkaline phosphatase activity, an important biological marker of bone tissue growth.
33652598	9	11	theme	form-increasing	1337:1351	arg1	convenience					1357:1367	its form-increasing its convenience	1333:1367	its form-increasing its convenience for application in surgery	1333:1394	Moreover, the granulate is suitable for thermal sterilization without losing its form-increasing its convenience for application in surgery for guided bone regeneration in case of minor or non-load bearing voids in bone tissue.
33652598	5	12	theme	porous	738:743	arg1	structure					745:753	an interconnected porous structure	720:753	an interconnected porous structure	720:753	To repair bone losses, the granulate form of the material is easy to apply and forms an interconnected porous structure.
33652598	9	13	theme	guided	1400:1405	arg1	regeneration					1412:1423	guided bone regeneration	1400:1423	guided bone regeneration in case of minor or non-load bearing voids in bone tissue	1400:1481	Moreover, the granulate is suitable for thermal sterilization without losing its form-increasing its convenience for application in surgery for guided bone regeneration in case of minor or non-load bearing voids in bone tissue.
33652598	3	14	theme	human	406:410	arg1	bone					412:415	human bone	406:415	human bone from tissue banks	406:433	However, human bone from tissue banks can be used to prepare materials in eligible form for transplantation.
33652598	5	15	theme	material	684:691	arg1	easy					696:699	easy	696:699	easy	696:699	To repair bone losses, the granulate form of the material is easy to apply and forms an interconnected porous structure.
33652598	5	15	theme	material	684:691	arg1	form					672:675	the granulate form	658:675	the granulate form of the material	658:691	To repair bone losses, the granulate form of the material is easy to apply and forms an interconnected porous structure.
33652598	4	16	theme	non-immunogenic	552:566	arg1	matrix					568:573	a non-immunogenic matrix	550:573	a non-immunogenic matrix for human cells to repopulate in the place of implantation	550:632	Without proteins and fats, the bone becomes a non-immunogenic matrix for human cells to repopulate in the place of implantation.
33652598	0	17	theme	Bone	15:18	arg1	Composite					20:28	Chitosan-Human Bone Composite	0:28	Chitosan-Human Bone Composite	0:28	Chitosan-Human Bone Composite Granulates for Guided Bone Regeneration.
33652598	7	18	theme	pH	1037:1038	arg1	increase					1040:1047	pH increase	1037:1047	pH increase	1037:1047	A commercial encapsulator was used to obtain granulate, using chitosan gelation upon pH increase.
33652598	4	19	theme	implantation	621:632	arg1	place					612:616	the place	608:616	the place of implantation	608:632	Without proteins and fats, the bone becomes a non-immunogenic matrix for human cells to repopulate in the place of implantation.
33652598	0	20	theme	Chitosan-Human	0:13	arg1	Composite					20:28	Chitosan-Human Bone Composite	0:28	Chitosan-Human Bone Composite	0:28	Chitosan-Human Bone Composite Granulates for Guided Bone Regeneration.
33652598	6	21	theme	tissue	871:876	arg1	engineering					878:888	tissue engineering	871:888	tissue engineering	871:888	A granulate composed of β-tricalcium phosphate, pulverized human bone, and chitosan-a potent biopolymer applied in tissue engineering, regenerative medicine, and biotechnology-has been developed.
33652598	1	22	theme	perfect	90:96	arg1	material					109:116	the perfect bone graft material	86:116	the perfect bone graft material	86:116	The search for the perfect bone graft material is an important topic in material science and medicine.
33652598	8	23	theme	phosphatase	1180:1190	arg1	marker					1226:1231	an important biological marker	1202:1231	an important biological marker of bone tissue growth	1202:1253	The granulate has been proven in vitro to be non-cytotoxic, suitable for MG63 cell growth on its surface, and increasing alkaline phosphatase activity, an important biological marker of bone tissue growth.
33652598	8	23	theme	phosphatase	1180:1190	arg1	activity					1192:1199	alkaline phosphatase activity	1171:1199	alkaline phosphatase activity	1171:1199	The granulate has been proven in vitro to be non-cytotoxic, suitable for MG63 cell growth on its surface, and increasing alkaline phosphatase activity, an important biological marker of bone tissue growth.
33652598	8	24	theme	bone	1236:1239	arg1	growth					1248:1253	bone tissue growth	1236:1253	bone tissue growth	1236:1253	The granulate has been proven in vitro to be non-cytotoxic, suitable for MG63 cell growth on its surface, and increasing alkaline phosphatase activity, an important biological marker of bone tissue growth.
33652598	1	25	theme	bone	98:101	arg1	material					109:116	the perfect bone graft material	86:116	the perfect bone graft material	86:116	The search for the perfect bone graft material is an important topic in material science and medicine.
33652598	7	26	theme	commercial	954:963	arg1	encapsulator					965:976	A commercial encapsulator	952:976	A commercial encapsulator	952:976	A commercial encapsulator was used to obtain granulate, using chitosan gelation upon pH increase.
33652598	6	27	theme	human	815:819	arg1	bone					821:824	pulverized human bone	804:824	pulverized human bone	804:824	A granulate composed of β-tricalcium phosphate, pulverized human bone, and chitosan-a potent biopolymer applied in tissue engineering, regenerative medicine, and biotechnology-has been developed.
33652598	1	28	theme	graft	103:107	arg1	material					109:116	the perfect bone graft material	86:116	the perfect bone graft material	86:116	The search for the perfect bone graft material is an important topic in material science and medicine.
33652598	0	29	theme	Guided	45:50	arg1	Regeneration					57:68	Guided Bone Regeneration	45:68	Guided Bone Regeneration	45:68	Chitosan-Human Bone Composite Granulates for Guided Bone Regeneration.
33652598	9	30	from	convenience	1357:1367	arg1	surgery					1388:1394	surgery	1388:1394	surgery	1388:1394	Moreover, the granulate is suitable for thermal sterilization without losing its form-increasing its convenience for application in surgery for guided bone regeneration in case of minor or non-load bearing voids in bone tissue.
33652598	6	31	theme	pulverized	804:813	arg1	bone					821:824	pulverized human bone	804:824	pulverized human bone	804:824	A granulate composed of β-tricalcium phosphate, pulverized human bone, and chitosan-a potent biopolymer applied in tissue engineering, regenerative medicine, and biotechnology-has been developed.
33652598	3	32	used	used	442:445	arg2	bone					412:415	human bone	406:415	human bone from tissue banks	406:433	However, human bone from tissue banks can be used to prepare materials in eligible form for transplantation.
33652598	9	33	theme	bone	1407:1410	arg1	regeneration					1412:1423	guided bone regeneration	1400:1423	guided bone regeneration in case of minor or non-load bearing voids in bone tissue	1400:1481	Moreover, the granulate is suitable for thermal sterilization without losing its form-increasing its convenience for application in surgery for guided bone regeneration in case of minor or non-load bearing voids in bone tissue.
33652598	2	34	theme	bone	380:383	arg1	harvesting					385:394	bone harvesting	380:394	bone harvesting	380:394	Despite human bone being the ideal material, due to its composition, morphology, and familiarity with cells, autografts are widely considered demanding and cause additional stress to the patient because of bone harvesting.
33652598	8	35	theme	alkaline	1171:1178	arg1	phosphatase					1180:1190	alkaline phosphatase	1171:1190	alkaline phosphatase activity	1171:1199	The granulate has been proven in vitro to be non-cytotoxic, suitable for MG63 cell growth on its surface, and increasing alkaline phosphatase activity, an important biological marker of bone tissue growth.
33652598	6	36	theme	regenerative	891:902	arg1	medicine					904:911	regenerative medicine	891:911	regenerative medicine	891:911	A granulate composed of β-tricalcium phosphate, pulverized human bone, and chitosan-a potent biopolymer applied in tissue engineering, regenerative medicine, and biotechnology-has been developed.
33652598	5	37	theme	granulate	662:670	arg1	easy					696:699	easy	696:699	easy	696:699	To repair bone losses, the granulate form of the material is easy to apply and forms an interconnected porous structure.
33652598	5	37	theme	granulate	662:670	arg1	form					672:675	the granulate form	658:675	the granulate form of the material	658:691	To repair bone losses, the granulate form of the material is easy to apply and forms an interconnected porous structure.
33652598	8	38	theme	biological	1215:1224	arg1	marker					1226:1231	an important biological marker	1202:1231	an important biological marker of bone tissue growth	1202:1253	The granulate has been proven in vitro to be non-cytotoxic, suitable for MG63 cell growth on its surface, and increasing alkaline phosphatase activity, an important biological marker of bone tissue growth.
33652598	8	38	theme	biological	1215:1224	arg1	activity					1192:1199	alkaline phosphatase activity	1171:1199	alkaline phosphatase activity	1171:1199	The granulate has been proven in vitro to be non-cytotoxic, suitable for MG63 cell growth on its surface, and increasing alkaline phosphatase activity, an important biological marker of bone tissue growth.
33652598	4	39	theme	human	579:583	arg1	cells					585:589	human cells	579:589	human cells	579:589	Without proteins and fats, the bone becomes a non-immunogenic matrix for human cells to repopulate in the place of implantation.
33652598	3	40	theme	tissue	422:427	arg1	banks					429:433	tissue banks	422:433	tissue banks	422:433	However, human bone from tissue banks can be used to prepare materials in eligible form for transplantation.
33652598	9	41	theme	thermal	1296:1302	arg1	sterilization					1304:1316	thermal sterilization	1296:1316	thermal sterilization	1296:1316	Moreover, the granulate is suitable for thermal sterilization without losing its form-increasing its convenience for application in surgery for guided bone regeneration in case of minor or non-load bearing voids in bone tissue.
33652598	5	42	theme	interconnected	723:736	arg1	structure					745:753	an interconnected porous structure	720:753	an interconnected porous structure	720:753	To repair bone losses, the granulate form of the material is easy to apply and forms an interconnected porous structure.
33652598	7	43	theme	chitosan	1014:1021	arg1	gelation					1023:1030	chitosan gelation	1014:1030	chitosan gelation	1014:1030	A commercial encapsulator was used to obtain granulate, using chitosan gelation upon pH increase.
33652598	0	44	theme	Bone	52:55	arg1	Regeneration					57:68	Guided Bone Regeneration	45:68	Guided Bone Regeneration	45:68	Chitosan-Human Bone Composite Granulates for Guided Bone Regeneration.
33652598	6	45	theme	β-tricalcium	780:791	arg1	phosphate					793:801	β-tricalcium phosphate	780:801	β-tricalcium phosphate	780:801	A granulate composed of β-tricalcium phosphate, pulverized human bone, and chitosan-a potent biopolymer applied in tissue engineering, regenerative medicine, and biotechnology-has been developed.
33652598	2	46	theme	ideal	203:207	arg1	bone					188:191	human bone	182:191	human bone	182:191	Despite human bone being the ideal material, due to its composition, morphology, and familiarity with cells, autografts are widely considered demanding and cause additional stress to the patient because of bone harvesting.
33652598	2	46	theme	ideal	203:207	arg1	material					209:216	the ideal material	199:216	the ideal material	199:216	Despite human bone being the ideal material, due to its composition, morphology, and familiarity with cells, autografts are widely considered demanding and cause additional stress to the patient because of bone harvesting.
33652598	3	47	from	banks	429:433	arg1	bone					412:415	human bone	406:415	human bone from tissue banks	406:433	However, human bone from tissue banks can be used to prepare materials in eligible form for transplantation.
33652598	2	48	with	composition	230:240	arg1	cells					276:280	cells	276:280	cells	276:280	Despite human bone being the ideal material, due to its composition, morphology, and familiarity with cells, autografts are widely considered demanding and cause additional stress to the patient because of bone harvesting.
33652598	5	49	theme	repair	638:643	arg1	losses					650:655	repair bone losses	638:655	repair bone losses	638:655	To repair bone losses, the granulate form of the material is easy to apply and forms an interconnected porous structure.
33652598	8	50	theme	tissue	1241:1246	arg1	growth					1248:1253	bone tissue growth	1236:1253	bone tissue growth	1236:1253	The granulate has been proven in vitro to be non-cytotoxic, suitable for MG63 cell growth on its surface, and increasing alkaline phosphatase activity, an important biological marker of bone tissue growth.
33652598	8	51	theme	important	1205:1213	arg1	marker					1226:1231	an important biological marker	1202:1231	an important biological marker of bone tissue growth	1202:1253	The granulate has been proven in vitro to be non-cytotoxic, suitable for MG63 cell growth on its surface, and increasing alkaline phosphatase activity, an important biological marker of bone tissue growth.
33652598	8	51	theme	important	1205:1213	arg1	activity					1192:1199	alkaline phosphatase activity	1171:1199	alkaline phosphatase activity	1171:1199	The granulate has been proven in vitro to be non-cytotoxic, suitable for MG63 cell growth on its surface, and increasing alkaline phosphatase activity, an important biological marker of bone tissue growth.
33652598	5	52	theme	bone	645:648	arg1	losses					650:655	repair bone losses	638:655	repair bone losses	638:655	To repair bone losses, the granulate form of the material is easy to apply and forms an interconnected porous structure.
33652598	8	53	theme	MG63	1123:1126	arg1	growth					1133:1138	MG63 cell growth	1123:1138	MG63 cell growth on its surface	1123:1153	The granulate has been proven in vitro to be non-cytotoxic, suitable for MG63 cell growth on its surface, and increasing alkaline phosphatase activity, an important biological marker of bone tissue growth.
33652598	1	54	theme	important	124:132	arg1	search					75:80	The search	71:80	The search for the perfect bone graft material	71:116	The search for the perfect bone graft material is an important topic in material science and medicine.
33652598	1	54	theme	important	124:132	arg1	topic					134:138	an important topic	121:138	an important topic in material science and medicine	121:171	The search for the perfect bone graft material is an important topic in material science and medicine.
33652598	7	55	used	used	982:985	arg2	encapsulator					965:976	A commercial encapsulator	952:976	A commercial encapsulator	952:976	A commercial encapsulator was used to obtain granulate, using chitosan gelation upon pH increase.
33652598	2	56	with	morphology	243:252	arg1	cells					276:280	cells	276:280	cells	276:280	Despite human bone being the ideal material, due to its composition, morphology, and familiarity with cells, autografts are widely considered demanding and cause additional stress to the patient because of bone harvesting.
33652598	2	57	theme	human	182:186	arg1	bone					188:191	human bone	182:191	human bone	182:191	Despite human bone being the ideal material, due to its composition, morphology, and familiarity with cells, autografts are widely considered demanding and cause additional stress to the patient because of bone harvesting.
33652598	2	57	theme	human	182:186	arg1	material					209:216	the ideal material	199:216	the ideal material	199:216	Despite human bone being the ideal material, due to its composition, morphology, and familiarity with cells, autografts are widely considered demanding and cause additional stress to the patient because of bone harvesting.
33652598	3	58	theme	eligible	471:478	arg1	form					480:483	eligible form	471:483	eligible form for transplantation	471:503	However, human bone from tissue banks can be used to prepare materials in eligible form for transplantation.
33652598	2	59	with	familiarity	259:269	arg1	cells					276:280	cells	276:280	cells	276:280	Despite human bone being the ideal material, due to its composition, morphology, and familiarity with cells, autografts are widely considered demanding and cause additional stress to the patient because of bone harvesting.
33652598	2	60	theme	additional	336:345	arg1	stress					347:352	additional stress	336:352	additional stress to the patient	336:367	Despite human bone being the ideal material, due to its composition, morphology, and familiarity with cells, autografts are widely considered demanding and cause additional stress to the patient because of bone harvesting.
32302865	2	0	theme	antioxidant	576:586	arg1	status					588:593	immune and antioxidant status	565:593	status	588:593	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	4	1	theme	enzymes	944:950	arg1	genes					967:971	head kidney and the scavenging enzymes (cat, sod, gr) genes	913:971	head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver	913:984	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	2	2	theme	immune	565:570	arg1	status					588:593	immune and antioxidant status	565:593	status	588:593	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	8	3	theme	present	1539:1545	arg1	findings					1547:1554	The present findings	1535:1554	The present findings	1535:1554	The present findings provided evidence that the tested EPSs with antibacterial and antioxidant activities can enhance immune response without negative effect on the biochemical composition.
32302865	2	4	theme	sea	607:609	arg1	bass					611:614	European sea bass	598:614	European sea bass (Dicentrarchus labrax L.) larvae	598:647	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	2	4	theme	sea	607:609	arg1	labrax					631:636	Dicentrarchus labrax L.	617:639	Dicentrarchus labrax L.	617:639	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	5	5	from	genes	1120:1124	arg1	head-kidney					1133:1143	the head-kidney	1129:1143	the head-kidney	1129:1143	In fact, the dietary supplementation with the tested EPSs, significantly enhances the expression of immune-associated genes in the head-kidney, particularly infg and tcrβ, as well as catalase gene in liver.
32302865	0	6	theme	Dietary	0:6	arg1	effects					23:29	Dietary administration effects	0:29	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).	0:186	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).
32302865	4	7	dep	kidney	918:923	arg1	cat					953:955	cat	953:955	cat	953:955	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	4	7	dep	kidney	918:923	arg1	gr					963:964	gr	963:964	gr	963:964	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	4	7	dep	kidney	918:923	arg1	sod					958:960	sod	958:960	sod	958:960	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	5	8	with	supplementation	1023:1037	arg1	EPSs					1055:1058	the tested EPSs	1044:1058	the tested EPSs	1044:1058	In fact, the dietary supplementation with the tested EPSs, significantly enhances the expression of immune-associated genes in the head-kidney, particularly infg and tcrβ, as well as catalase gene in liver.
32302865	4	9	theme	genes	967:971	arg1	levels					833:838	expression levels	822:838	expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver	822:984	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	8	10	with	EPSs	1590:1593	arg1	activities					1630:1639	antibacterial and antioxidant activities	1600:1639	antibacterial and antioxidant activities	1600:1639	The present findings provided evidence that the tested EPSs with antibacterial and antioxidant activities can enhance immune response without negative effect on the biochemical composition.
32302865	8	11	theme	tested	1583:1588	arg1	EPSs					1590:1593	the tested EPSs	1579:1593	the tested EPSs with antibacterial and antioxidant activities	1579:1639	The present findings provided evidence that the tested EPSs with antibacterial and antioxidant activities can enhance immune response without negative effect on the biochemical composition.
32302865	2	12	theme	EPB	516:518	arg1	"					519:519	"EPB"	515:519	"EPB"	515:519	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	9	13	theme	aquafeed	1796:1803	arg1	ingredients					1805:1815	natural functional aquafeed ingredients	1777:1815	natural functional aquafeed ingredients for European sea bass	1777:1837	The used EPSs can be considered as a good source of natural functional aquafeed ingredients for European sea bass.
32302865	0	14	theme	European	143:150	arg1	bass					156:159	European sea bass	143:159	European sea bass (Dicentrarchus labrax L.)	143:185	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).
32302865	2	15	theme	potential	433:441	arg1	effect					443:448	the potential effect	429:448	the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae	429:647	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	4	16	from	genes	967:971	arg1	liver					980:984	the liver	976:984	the liver	976:984	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	4	16	from	genes	967:971	arg1	gut					905:907	the gut	901:907	the gut	901:907	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	0	17	from	strains	77:83	arg1	effects					23:29	Dietary administration effects	0:29	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).	0:186	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).
32302865	0	17	from	strains	77:83	arg1	status					111:116	immune and antioxidant status	88:116	status	111:116	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).
32302865	0	17	from	strains	77:83	arg1	value					134:138	nutritional value	122:138	nutritional value of European sea bass (Dicentrarchus labrax L.)	122:185	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).
32302865	2	18	theme	potential	534:542	arg1	strains					554:560	potential probiotic strains	534:560	potential probiotic strains	534:560	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	0	19	theme	bass	156:159	arg1	status					111:116	immune and antioxidant status	88:116	status	111:116	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).
32302865	0	19	theme	bass	156:159	arg1	value					134:138	nutritional value	122:138	nutritional value of European sea bass (Dicentrarchus labrax L.)	122:185	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).
32302865	4	20	from	genes	859:863	arg1	liver					980:984	the liver	976:984	the liver	976:984	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	4	20	from	genes	859:863	arg1	gut					905:907	the gut	901:907	the gut	901:907	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	4	21	theme	expression	822:831	arg1	levels					833:838	expression levels	822:838	expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver	822:984	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	4	22	theme	genes	859:863	arg1	levels					833:838	expression levels	822:838	expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver	822:984	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	5	23	from	gene	1194:1197	arg1	head-kidney					1133:1143	the head-kidney	1129:1143	the head-kidney	1129:1143	In fact, the dietary supplementation with the tested EPSs, significantly enhances the expression of immune-associated genes in the head-kidney, particularly infg and tcrβ, as well as catalase gene in liver.
32302865	9	24	theme	sea	1830:1832	arg1	bass					1834:1837	European sea bass	1821:1837	European sea bass	1821:1837	The used EPSs can be considered as a good source of natural functional aquafeed ingredients for European sea bass.
32302865	0	25	theme	Dicentrarchus	162:174	arg1	labrax					176:181	Dicentrarchus labrax	162:181	Dicentrarchus labrax L.	162:184	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).
32302865	0	26	dep	bass	156:159	arg1	labrax					176:181	Dicentrarchus labrax	162:181	Dicentrarchus labrax L.	162:184	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).
32302865	6	27	theme	study	1230:1234	arg1	period					1220:1225	the period	1216:1225	the period of study	1216:1234	During the period of study, EPSs administration did not affect the fatty acid profiles of larvae, which is balanced.
32302865	5	28	from	expression	1088:1097	arg1	liver					1202:1206	liver	1202:1206	liver	1202:1206	In fact, the dietary supplementation with the tested EPSs, significantly enhances the expression of immune-associated genes in the head-kidney, particularly infg and tcrβ, as well as catalase gene in liver.
32302865	5	28	from	expression	1088:1097	arg1	head-kidney					1133:1143	the head-kidney	1129:1143	the head-kidney	1129:1143	In fact, the dietary supplementation with the tested EPSs, significantly enhances the expression of immune-associated genes in the head-kidney, particularly infg and tcrβ, as well as catalase gene in liver.
32302865	0	29	theme	immune	88:93	arg1	status					111:116	immune and antioxidant status	88:116	status	111:116	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).
32302865	2	30	dep	labrax	631:636	arg1	L.					638:639	Dicentrarchus labrax L.	617:639	Dicentrarchus labrax L.	617:639	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	2	31	from	effect	443:448	arg1	status					588:593	immune and antioxidant status	565:593	status	588:593	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	2	32	theme	Dicentrarchus	617:629	arg1	bass					611:614	European sea bass	598:614	European sea bass (Dicentrarchus labrax L.) larvae	598:647	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	2	32	theme	Dicentrarchus	617:629	arg1	labrax					631:636	Dicentrarchus labrax L.	617:639	Dicentrarchus labrax L.	617:639	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	0	33	theme	antioxidant	99:109	arg1	status					111:116	immune and antioxidant status	88:116	status	111:116	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).
32302865	8	34	theme	negative	1677:1684	arg1	effect					1686:1691	negative effect	1677:1691	negative effect on the biochemical composition	1677:1722	The present findings provided evidence that the tested EPSs with antibacterial and antioxidant activities can enhance immune response without negative effect on the biochemical composition.
32302865	7	35	theme	bass	1529:1532	arg1	composition					1501:1511	biochemical composition	1489:1511	biochemical composition of European sea bass	1489:1532	This is confirmed by the Docosahexaenoic acid / Eicosapentaenoic acid ratio and demonstrates that EPLB and EPB can be administrated without any negative effect on biochemical composition of European sea bass.
32302865	7	36	theme	acid	1391:1394	arg1	ratio					1396:1400	the Docosahexaenoic acid / Eicosapentaenoic acid ratio	1347:1400	the Docosahexaenoic acid / Eicosapentaenoic acid ratio	1347:1400	This is confirmed by the Docosahexaenoic acid / Eicosapentaenoic acid ratio and demonstrates that EPLB and EPB can be administrated without any negative effect on biochemical composition of European sea bass.
32302865	2	37	theme	EPLB	505:508	arg1	"					509:509	"EPLB"	504:509	"EPLB"	504:509	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	2	38	theme	bass	611:614	arg1	larvae					642:647	European sea bass (Dicentrarchus labrax L.) larvae	598:647	European sea bass (Dicentrarchus labrax L.) larvae	598:647	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	5	39	theme	catalase	1185:1192	arg1	gene					1194:1197	catalase gene	1185:1197	catalase gene	1185:1197	In fact, the dietary supplementation with the tested EPSs, significantly enhances the expression of immune-associated genes in the head-kidney, particularly infg and tcrβ, as well as catalase gene in liver.
32302865	7	40	theme	European	1516:1523	arg1	bass					1529:1532	European sea bass	1516:1532	European sea bass	1516:1532	This is confirmed by the Docosahexaenoic acid / Eicosapentaenoic acid ratio and demonstrates that EPLB and EPB can be administrated without any negative effect on biochemical composition of European sea bass.
32302865	1	41	theme	aquaculture	294:304	arg1	sector					306:311	aquaculture sector	294:311	aquaculture sector that may help to increase production and maintain healthy environment	294:381	The use of biological immunostimulants is considered a valuable practice to improve culture conditions in aquaculture sector that may help to increase production and maintain healthy environment.
32302865	2	42	theme	larvae	642:647	arg1	status					588:593	immune and antioxidant status	565:593	status	588:593	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	6	43	theme	fatty	1276:1280	arg1	profiles					1287:1294	the fatty acid profiles	1272:1294	the fatty acid profiles	1272:1294	During the period of study, EPSs administration did not affect the fatty acid profiles of larvae, which is balanced.
32302865	6	43	theme	fatty	1276:1280	arg1	balanced					1316:1323	balanced	1316:1323	balanced	1316:1323	During the period of study, EPSs administration did not affect the fatty acid profiles of larvae, which is balanced.
32302865	1	44	theme	culture	272:278	arg1	conditions					280:289	culture conditions	272:289	culture conditions	272:289	The use of biological immunostimulants is considered a valuable practice to improve culture conditions in aquaculture sector that may help to increase production and maintain healthy environment.
32302865	7	45	from	effect	1479:1484	arg1	composition					1501:1511	biochemical composition	1489:1511	biochemical composition of European sea bass	1489:1532	This is confirmed by the Docosahexaenoic acid / Eicosapentaenoic acid ratio and demonstrates that EPLB and EPB can be administrated without any negative effect on biochemical composition of European sea bass.
32302865	8	46	theme	biochemical	1700:1710	arg1	composition					1712:1722	the biochemical composition	1696:1722	the biochemical composition	1696:1722	The present findings provided evidence that the tested EPSs with antibacterial and antioxidant activities can enhance immune response without negative effect on the biochemical composition.
32302865	4	47	theme	scavenging	933:942	arg1	enzymes					944:950	the scavenging enzymes	929:950	the scavenging enzymes	929:950	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	7	48	theme	acid	1367:1370	arg1	ratio					1396:1400	the Docosahexaenoic acid / Eicosapentaenoic acid ratio	1347:1400	the Docosahexaenoic acid / Eicosapentaenoic acid ratio	1347:1400	This is confirmed by the Docosahexaenoic acid / Eicosapentaenoic acid ratio and demonstrates that EPLB and EPB can be administrated without any negative effect on biochemical composition of European sea bass.
32302865	0	49	theme	exopolysaccharide	34:50	arg1	effects					23:29	Dietary administration effects	0:29	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).	0:186	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).
32302865	4	50	theme	head	913:916	arg1	kidney					918:923	head kidney	913:923	head kidney	913:923	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	6	51	theme	EPSs	1237:1240	arg1	administration					1242:1255	EPSs administration	1237:1255	EPSs administration	1237:1255	During the period of study, EPSs administration did not affect the fatty acid profiles of larvae, which is balanced.
32302865	4	52	from	gut	905:907	arg1	levels					833:838	expression levels	822:838	expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver	822:984	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	2	53	dep	exopolysaccharides	479:496	arg1	"					519:519	"EPB"	515:519	"EPB"	515:519	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	2	53	dep	exopolysaccharides	479:496	arg1	"					509:509	"EPLB"	504:509	"EPLB"	504:509	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	1	54	theme	immunostimulants	210:225	arg1	use					192:194	The use	188:194	The use of biological immunostimulants	188:225	The use of biological immunostimulants is considered a valuable practice to improve culture conditions in aquaculture sector that may help to increase production and maintain healthy environment.
32302865	2	55	theme	administration	457:470	arg1	effect					443:448	the potential effect	429:448	the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae	429:647	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	6	56	theme	larvae	1299:1304	arg1	profiles					1287:1294	the fatty acid profiles	1272:1294	the fatty acid profiles	1272:1294	During the period of study, EPSs administration did not affect the fatty acid profiles of larvae, which is balanced.
32302865	6	56	theme	larvae	1299:1304	arg1	balanced					1316:1323	balanced	1316:1323	balanced	1316:1323	During the period of study, EPSs administration did not affect the fatty acid profiles of larvae, which is balanced.
32302865	1	57	theme	valuable	243:250	arg1	practice					252:259	a valuable practice	241:259	a valuable practice to improve culture conditions in aquaculture sector that may help to increase production and maintain healthy environment	241:381	The use of biological immunostimulants is considered a valuable practice to improve culture conditions in aquaculture sector that may help to increase production and maintain healthy environment.
32302865	5	58	from	head-kidney	1133:1143	arg1	expression					1088:1097	the expression	1084:1097	the expression of immune-associated genes in the head-kidney, particularly infg and tcrβ, as well as catalase gene in liver	1084:1206	In fact, the dietary supplementation with the tested EPSs, significantly enhances the expression of immune-associated genes in the head-kidney, particularly infg and tcrβ, as well as catalase gene in liver.
32302865	0	59	theme	potential	57:65	arg1	strains					77:83	potential probiotic strains	57:83	potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.)	57:185	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).
32302865	2	60	theme	European	598:605	arg1	bass					611:614	European sea bass	598:614	European sea bass (Dicentrarchus labrax L.) larvae	598:647	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	2	60	theme	European	598:605	arg1	labrax					631:636	Dicentrarchus labrax L.	617:639	Dicentrarchus labrax L.	617:639	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	9	61	theme	good	1762:1765	arg1	source					1767:1772	a good source	1760:1772	a good source of natural functional aquafeed ingredients for European sea bass	1760:1837	The used EPSs can be considered as a good source of natural functional aquafeed ingredients for European sea bass.
32302865	9	61	theme	good	1762:1765	arg1	EPSs					1734:1737	The used EPSs	1725:1737	The used EPSs	1725:1737	The used EPSs can be considered as a good source of natural functional aquafeed ingredients for European sea bass.
32302865	7	62	theme	negative	1470:1477	arg1	effect					1479:1484	any negative effect	1466:1484	any negative effect on biochemical composition of European sea bass	1466:1532	This is confirmed by the Docosahexaenoic acid / Eicosapentaenoic acid ratio and demonstrates that EPLB and EPB can be administrated without any negative effect on biochemical composition of European sea bass.
32302865	2	63	attach	derived	521:527	arg2	exopolysaccharides					479:496	two exopolysaccharides	475:496	two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains	475:560	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	2	63	attach	derived	521:527	arg2	EPS					499:501	EPS	499:501	EPS	499:501	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	2	63	attach	derived	521:527	arg1	strains					554:560	potential probiotic strains	534:560	potential probiotic strains	534:560	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	4	64	from	liver	980:984	arg1	levels					833:838	expression levels	822:838	expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver	822:984	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	9	65	theme	functional	1785:1794	arg1	ingredients					1805:1815	natural functional aquafeed ingredients	1777:1815	natural functional aquafeed ingredients for European sea bass	1777:1837	The used EPSs can be considered as a good source of natural functional aquafeed ingredients for European sea bass.
32302865	0	66	theme	sea	152:154	arg1	bass					156:159	European sea bass	143:159	European sea bass (Dicentrarchus labrax L.)	143:185	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).
32302865	9	67	theme	ingredients	1805:1815	arg1	source					1767:1772	a good source	1760:1772	a good source of natural functional aquafeed ingredients for European sea bass	1760:1837	The used EPSs can be considered as a good source of natural functional aquafeed ingredients for European sea bass.
32302865	9	67	theme	ingredients	1805:1815	arg1	EPSs					1734:1737	The used EPSs	1725:1737	The used EPSs	1725:1737	The used EPSs can be considered as a good source of natural functional aquafeed ingredients for European sea bass.
32302865	0	68	dep	labrax	176:181	arg1	L					183:183	L	183:183	Dicentrarchus labrax L.	162:184	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).
32302865	2	69	theme	probiotic	544:552	arg1	strains					554:560	potential probiotic strains	534:560	potential probiotic strains	534:560	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	9	70	theme	European	1821:1828	arg1	bass					1834:1837	European sea bass	1821:1837	European sea bass	1821:1837	The used EPSs can be considered as a good source of natural functional aquafeed ingredients for European sea bass.
32302865	3	71	theme	biochemical	705:715	arg1	composition					717:727	the biochemical composition	701:727	the biochemical composition during the trial period	701:751	In order to find out if the EPSs have an effect on the biochemical composition during the trial period, the nutritional value has been evaluated.
32302865	8	72	theme	antibacterial	1600:1612	arg1	activities					1630:1639	antibacterial and antioxidant activities	1600:1639	antibacterial and antioxidant activities	1600:1639	The present findings provided evidence that the tested EPSs with antibacterial and antioxidant activities can enhance immune response without negative effect on the biochemical composition.
32302865	4	73	theme	immune-relevant	843:857	arg1	infg					866:869	infg	866:869	infg	866:869	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	4	73	theme	immune-relevant	843:857	arg1	Il1b					872:875	Il1b	872:875	Il1b	872:875	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	4	73	theme	immune-relevant	843:857	arg1	tcr-β					891:895	tcr-β	891:895	tcr-β	891:895	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	4	73	theme	immune-relevant	843:857	arg1	genes					859:863	immune-relevant genes	843:863	immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut	843:907	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	4	73	theme	immune-relevant	843:857	arg1	Il8					878:880	Il8	878:880	Il8	878:880	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	4	73	theme	immune-relevant	843:857	arg1	Il6					883:885	Il6	883:885	Il6	883:885	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	9	74	theme	natural	1777:1783	arg1	ingredients					1805:1815	natural functional aquafeed ingredients	1777:1815	natural functional aquafeed ingredients for European sea bass	1777:1837	The used EPSs can be considered as a good source of natural functional aquafeed ingredients for European sea bass.
32302865	4	75	dep	genes	859:863	arg1	infg					866:869	infg	866:869	infg	866:869	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	4	75	dep	genes	859:863	arg1	Il1b					872:875	Il1b	872:875	Il1b	872:875	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	4	75	dep	genes	859:863	arg1	tcr-β					891:895	tcr-β	891:895	tcr-β	891:895	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	4	75	dep	genes	859:863	arg1	genes					859:863	immune-relevant genes	843:863	immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut	843:907	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	4	75	dep	genes	859:863	arg1	Il8					878:880	Il8	878:880	Il8	878:880	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	4	75	dep	genes	859:863	arg1	Il6					883:885	Il6	883:885	Il6	883:885	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	4	76	from	levels	833:838	arg1	liver					980:984	the liver	976:984	the liver	976:984	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	4	76	from	levels	833:838	arg1	gut					905:907	the gut	901:907	the gut	901:907	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	8	77	theme	antioxidant	1618:1628	arg1	activities					1630:1639	antibacterial and antioxidant activities	1600:1639	antibacterial and antioxidant activities	1600:1639	The present findings provided evidence that the tested EPSs with antibacterial and antioxidant activities can enhance immune response without negative effect on the biochemical composition.
32302865	5	78	theme	genes	1120:1124	arg1	expression					1088:1097	the expression	1084:1097	the expression of immune-associated genes in the head-kidney, particularly infg and tcrβ, as well as catalase gene in liver	1084:1206	In fact, the dietary supplementation with the tested EPSs, significantly enhances the expression of immune-associated genes in the head-kidney, particularly infg and tcrβ, as well as catalase gene in liver.
32302865	3	79	theme	trial	740:744	arg1	period					746:751	the trial period	736:751	the trial period	736:751	In order to find out if the EPSs have an effect on the biochemical composition during the trial period, the nutritional value has been evaluated.
32302865	2	80	theme	exopolysaccharides	479:496	arg1	administration					457:470	the administration	453:470	the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains	453:560	We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.
32302865	8	81	from	effect	1686:1691	arg1	composition					1712:1722	the biochemical composition	1696:1722	the biochemical composition	1696:1722	The present findings provided evidence that the tested EPSs with antibacterial and antioxidant activities can enhance immune response without negative effect on the biochemical composition.
32302865	8	82	theme	immune	1653:1658	arg1	response					1660:1667	immune response	1653:1667	immune response	1653:1667	The present findings provided evidence that the tested EPSs with antibacterial and antioxidant activities can enhance immune response without negative effect on the biochemical composition.
32302865	7	83	theme	Eicosapentaenoic	1374:1389	arg1	acid					1391:1394	Eicosapentaenoic acid	1374:1394	the Docosahexaenoic acid / Eicosapentaenoic acid ratio	1347:1400	This is confirmed by the Docosahexaenoic acid / Eicosapentaenoic acid ratio and demonstrates that EPLB and EPB can be administrated without any negative effect on biochemical composition of European sea bass.
32302865	3	84	theme	nutritional	758:768	arg1	value					770:774	the nutritional value	754:774	the nutritional value	754:774	In order to find out if the EPSs have an effect on the biochemical composition during the trial period, the nutritional value has been evaluated.
32302865	0	85	theme	nutritional	122:132	arg1	value					134:138	nutritional value	122:138	nutritional value of European sea bass (Dicentrarchus labrax L.)	122:185	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).
32302865	1	86	theme	healthy	363:369	arg1	environment					371:381	healthy environment	363:381	healthy environment	363:381	The use of biological immunostimulants is considered a valuable practice to improve culture conditions in aquaculture sector that may help to increase production and maintain healthy environment.
32302865	5	87	theme	gene	1194:1197	arg1	expression					1088:1097	the expression	1084:1097	the expression of immune-associated genes in the head-kidney, particularly infg and tcrβ, as well as catalase gene in liver	1084:1206	In fact, the dietary supplementation with the tested EPSs, significantly enhances the expression of immune-associated genes in the head-kidney, particularly infg and tcrβ, as well as catalase gene in liver.
32302865	0	88	theme	administration	8:21	arg1	effects					23:29	Dietary administration effects	0:29	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).	0:186	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).
32302865	7	89	theme	sea	1525:1527	arg1	bass					1529:1532	European sea bass	1516:1532	European sea bass	1516:1532	This is confirmed by the Docosahexaenoic acid / Eicosapentaenoic acid ratio and demonstrates that EPLB and EPB can be administrated without any negative effect on biochemical composition of European sea bass.
32302865	4	90	theme	kidney	918:923	arg1	genes					967:971	head kidney and the scavenging enzymes (cat, sod, gr) genes	913:971	head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver	913:984	The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated.
32302865	3	91	contain	have	683:686	arg2	effect					691:696	an effect	688:696	an effect	688:696	In order to find out if the EPSs have an effect on the biochemical composition during the trial period, the nutritional value has been evaluated.
32302865	3	91	contain	have	683:686	arg1	EPSs					678:681	the EPSs	674:681	the EPSs	674:681	In order to find out if the EPSs have an effect on the biochemical composition during the trial period, the nutritional value has been evaluated.
32302865	5	92	theme	tested	1048:1053	arg1	EPSs					1055:1058	the tested EPSs	1044:1058	the tested EPSs	1044:1058	In fact, the dietary supplementation with the tested EPSs, significantly enhances the expression of immune-associated genes in the head-kidney, particularly infg and tcrβ, as well as catalase gene in liver.
32302865	7	93	theme	biochemical	1489:1499	arg1	composition					1501:1511	biochemical composition	1489:1511	biochemical composition of European sea bass	1489:1532	This is confirmed by the Docosahexaenoic acid / Eicosapentaenoic acid ratio and demonstrates that EPLB and EPB can be administrated without any negative effect on biochemical composition of European sea bass.
32302865	0	94	theme	probiotic	67:75	arg1	strains					77:83	potential probiotic strains	57:83	potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.)	57:185	Dietary administration effects of exopolysaccharide from potential probiotic strains on immune and antioxidant status and nutritional value of European sea bass (Dicentrarchus labrax L.).
32302865	7	95	theme	Docosahexaenoic	1351:1365	arg1	acid					1367:1370	Docosahexaenoic acid	1351:1370	the Docosahexaenoic acid / Eicosapentaenoic acid ratio	1347:1400	This is confirmed by the Docosahexaenoic acid / Eicosapentaenoic acid ratio and demonstrates that EPLB and EPB can be administrated without any negative effect on biochemical composition of European sea bass.
32302865	5	96	theme	dietary	1015:1021	arg1	supplementation					1023:1037	the dietary supplementation	1011:1037	the dietary supplementation	1011:1037	In fact, the dietary supplementation with the tested EPSs, significantly enhances the expression of immune-associated genes in the head-kidney, particularly infg and tcrβ, as well as catalase gene in liver.
32302865	1	97	theme	biological	199:208	arg1	immunostimulants					210:225	biological immunostimulants	199:225	biological immunostimulants	199:225	The use of biological immunostimulants is considered a valuable practice to improve culture conditions in aquaculture sector that may help to increase production and maintain healthy environment.
32302865	9	98	theme	used	1729:1732	arg1	source					1767:1772	a good source	1760:1772	a good source of natural functional aquafeed ingredients for European sea bass	1760:1837	The used EPSs can be considered as a good source of natural functional aquafeed ingredients for European sea bass.
32302865	9	98	theme	used	1729:1732	arg1	EPSs					1734:1737	The used EPSs	1725:1737	The used EPSs	1725:1737	The used EPSs can be considered as a good source of natural functional aquafeed ingredients for European sea bass.
32302865	6	99	theme	acid	1282:1285	arg1	profiles					1287:1294	the fatty acid profiles	1272:1294	the fatty acid profiles	1272:1294	During the period of study, EPSs administration did not affect the fatty acid profiles of larvae, which is balanced.
32302865	6	99	theme	acid	1282:1285	arg1	balanced					1316:1323	balanced	1316:1323	balanced	1316:1323	During the period of study, EPSs administration did not affect the fatty acid profiles of larvae, which is balanced.
32302865	5	100	theme	immune-associated	1102:1118	arg1	genes					1120:1124	immune-associated genes	1102:1124	immune-associated genes in the head-kidney	1102:1143	In fact, the dietary supplementation with the tested EPSs, significantly enhances the expression of immune-associated genes in the head-kidney, particularly infg and tcrβ, as well as catalase gene in liver.
32302865	5	100	theme	immune-associated	1102:1118	arg1	infg					1159:1162	infg	1159:1162	infg	1159:1162	In fact, the dietary supplementation with the tested EPSs, significantly enhances the expression of immune-associated genes in the head-kidney, particularly infg and tcrβ, as well as catalase gene in liver.
32302865	5	100	theme	immune-associated	1102:1118	arg1	tcrβ					1168:1171	tcrβ	1168:1171	tcrβ	1168:1171	In fact, the dietary supplementation with the tested EPSs, significantly enhances the expression of immune-associated genes in the head-kidney, particularly infg and tcrβ, as well as catalase gene in liver.
33259842	0	0	theme	full-thickness	116:129	arg1	healing					137:143	full-thickness wound healing	116:143	full-thickness wound healing	116:143	A biodegradable antibacterial alginate/carboxymethyl chitosan/Kangfuxin sponges for promoting blood coagulation and full-thickness wound healing.
33259842	6	1	contain	has	1218:1220	arg2	promise					1228:1234	great promise	1222:1234	great promise for the application of full-thickness wound healing	1222:1286	Thus, the multifunctional ACK-10 composite sponge has great promise for the application of full-thickness wound healing.
33259842	6	1	contain	has	1218:1220	arg1	sponge					1211:1216	the multifunctional ACK-10 composite sponge	1174:1216	the multifunctional ACK-10 composite sponge	1174:1216	Thus, the multifunctional ACK-10 composite sponge has great promise for the application of full-thickness wound healing.
33259842	5	2	theme	%	1161:1161	arg1	KFX					1163:1165	15% KFX	1159:1165	15% KFX	1159:1165	Further, in a rat full-thickness wounds model, the ACK sponge containing 10% KFX (ACK-10) significantly facilitates wound closure compared to the AC group and ACK sponge containing 5% and 15% KFX.
33259842	4	3	theme	rapid	953:957	arg1	hemostasis					959:968	rapid hemostasis	953:968	rapid hemostasis	953:968	It is demonstrated that the AG/CMC/KFX (ACK) sponges exhibit a highly interconnected and porous structure, suitable water vapor transmittance, excellent elastic properties, antibacterial behavior, cytocompatibility, and rapid hemostasis.
33259842	4	4	theme	ACK	773:775	arg1	sponges					778:784	the AG/CMC/KFX (ACK) sponges	757:784	the AG/CMC/KFX (ACK) sponges	757:784	It is demonstrated that the AG/CMC/KFX (ACK) sponges exhibit a highly interconnected and porous structure, suitable water vapor transmittance, excellent elastic properties, antibacterial behavior, cytocompatibility, and rapid hemostasis.
33259842	6	5	theme	wound	1274:1278	arg1	healing					1280:1286	full-thickness wound healing	1259:1286	full-thickness wound healing	1259:1286	Thus, the multifunctional ACK-10 composite sponge has great promise for the application of full-thickness wound healing.
33259842	4	6	theme	suitable	840:847	arg1	vapor					855:859	suitable water vapor	840:859	suitable water vapor transmittance	840:873	It is demonstrated that the AG/CMC/KFX (ACK) sponges exhibit a highly interconnected and porous structure, suitable water vapor transmittance, excellent elastic properties, antibacterial behavior, cytocompatibility, and rapid hemostasis.
33259842	4	7	theme	porous	822:827	arg1	transmittance					861:873	suitable water vapor transmittance	840:873	suitable water vapor transmittance	840:873	It is demonstrated that the AG/CMC/KFX (ACK) sponges exhibit a highly interconnected and porous structure, suitable water vapor transmittance, excellent elastic properties, antibacterial behavior, cytocompatibility, and rapid hemostasis.
33259842	4	7	theme	porous	822:827	arg1	structure					829:837	a highly interconnected and porous structure	794:837	a highly interconnected and porous structure	794:837	It is demonstrated that the AG/CMC/KFX (ACK) sponges exhibit a highly interconnected and porous structure, suitable water vapor transmittance, excellent elastic properties, antibacterial behavior, cytocompatibility, and rapid hemostasis.
33259842	0	8	theme	wound	131:135	arg1	healing					137:143	full-thickness wound healing	116:143	full-thickness wound healing	116:143	A biodegradable antibacterial alginate/carboxymethyl chitosan/Kangfuxin sponges for promoting blood coagulation and full-thickness wound healing.
33259842	6	9	theme	ACK-10	1194:1199	arg1	sponge					1211:1216	the multifunctional ACK-10 composite sponge	1174:1216	the multifunctional ACK-10 composite sponge	1174:1216	Thus, the multifunctional ACK-10 composite sponge has great promise for the application of full-thickness wound healing.
33259842	3	10	dep	medicine	582:589	arg1	Kangfuxin					591:599	Kangfuxin	591:599	traditional Chinese medicine Kangfuxin (KFX)	562:605	Herein, multifunctional composite sponges were developed by incorporation of traditional Chinese medicine Kangfuxin (KFX) into alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods.
33259842	3	10	dep	medicine	582:589	arg1	KFX					602:604	KFX	602:604	KFX	602:604	Herein, multifunctional composite sponges were developed by incorporation of traditional Chinese medicine Kangfuxin (KFX) into alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods.
33259842	3	11	theme	/carboxymethyl	625:638	arg1	CMC					650:652	CMC	650:652	CMC	650:652	Herein, multifunctional composite sponges were developed by incorporation of traditional Chinese medicine Kangfuxin (KFX) into alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods.
33259842	3	11	theme	/carboxymethyl	625:638	arg1	chitosan					640:647	alginate (AG)/carboxymethyl chitosan	612:647	alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods	612:730	Herein, multifunctional composite sponges were developed by incorporation of traditional Chinese medicine Kangfuxin (KFX) into alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods.
33259842	3	12	theme	medicine	582:589	arg1	incorporation					545:557	incorporation	545:557	incorporation of traditional Chinese medicine Kangfuxin (KFX) into alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods	545:730	Herein, multifunctional composite sponges were developed by incorporation of traditional Chinese medicine Kangfuxin (KFX) into alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods.
33259842	5	13	theme	full-thickness	989:1002	arg1	model					1011:1015	a rat full-thickness wounds model	983:1015	a rat full-thickness wounds model	983:1015	Further, in a rat full-thickness wounds model, the ACK sponge containing 10% KFX (ACK-10) significantly facilitates wound closure compared to the AC group and ACK sponge containing 5% and 15% KFX.
33259842	5	14	theme	ACK	1022:1024	arg1	sponge					1026:1031	the ACK sponge	1018:1031	the ACK sponge containing 10% KFX (ACK-10)	1018:1059	Further, in a rat full-thickness wounds model, the ACK sponge containing 10% KFX (ACK-10) significantly facilitates wound closure compared to the AC group and ACK sponge containing 5% and 15% KFX.
33259842	2	15	theme	promising	342:350	arg1	strategy					352:359	a promising strategy	340:359	a promising strategy to accelerate blood coagulation, inhibit bacterial infection, and trigger full-thickness wound into a regenerative process	340:482	The development of multifunctional wound dressings is emerging as a promising strategy to accelerate blood coagulation, inhibit bacterial infection, and trigger full-thickness wound into a regenerative process.
33259842	5	16	theme	15	1159:1160	arg1	%					1161:1161	%	1161:1161	%	1161:1161	Further, in a rat full-thickness wounds model, the ACK sponge containing 10% KFX (ACK-10) significantly facilitates wound closure compared to the AC group and ACK sponge containing 5% and 15% KFX.
33259842	3	17	theme	AG	622:623	arg1	CMC					650:652	CMC	650:652	CMC	650:652	Herein, multifunctional composite sponges were developed by incorporation of traditional Chinese medicine Kangfuxin (KFX) into alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods.
33259842	3	17	theme	AG	622:623	arg1	chitosan					640:647	alginate (AG)/carboxymethyl chitosan	612:647	alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods	612:730	Herein, multifunctional composite sponges were developed by incorporation of traditional Chinese medicine Kangfuxin (KFX) into alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods.
33259842	2	18	theme	regenerative	463:474	arg1	process					476:482	a regenerative process	461:482	a regenerative process	461:482	The development of multifunctional wound dressings is emerging as a promising strategy to accelerate blood coagulation, inhibit bacterial infection, and trigger full-thickness wound into a regenerative process.
33259842	0	19	theme	biodegradable	2:14	arg1	alginate/carboxymethyl					30:51	A biodegradable antibacterial alginate/carboxymethyl	0:51	A biodegradable antibacterial alginate/carboxymethyl	0:51	A biodegradable antibacterial alginate/carboxymethyl chitosan/Kangfuxin sponges for promoting blood coagulation and full-thickness wound healing.
33259842	3	20	theme	alginate	612:619	arg1	CMC					650:652	CMC	650:652	CMC	650:652	Herein, multifunctional composite sponges were developed by incorporation of traditional Chinese medicine Kangfuxin (KFX) into alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods.
33259842	3	20	theme	alginate	612:619	arg1	chitosan					640:647	alginate (AG)/carboxymethyl chitosan	612:647	alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods	612:730	Herein, multifunctional composite sponges were developed by incorporation of traditional Chinese medicine Kangfuxin (KFX) into alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods.
33259842	6	21	theme	composite	1201:1209	arg1	sponge					1211:1216	the multifunctional ACK-10 composite sponge	1174:1216	the multifunctional ACK-10 composite sponge	1174:1216	Thus, the multifunctional ACK-10 composite sponge has great promise for the application of full-thickness wound healing.
33259842	3	22	theme	composite	509:517	arg1	sponges					519:525	multifunctional composite sponges	493:525	multifunctional composite sponges	493:525	Herein, multifunctional composite sponges were developed by incorporation of traditional Chinese medicine Kangfuxin (KFX) into alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods.
33259842	5	23	contain	containing	1141:1150	arg1	group					1120:1124	the AC group	1113:1124	the AC group	1113:1124	Further, in a rat full-thickness wounds model, the ACK sponge containing 10% KFX (ACK-10) significantly facilitates wound closure compared to the AC group and ACK sponge containing 5% and 15% KFX.
33259842	5	23	contain	containing	1141:1150	arg2	%					1153:1153	5%	1152:1153	5%	1152:1153	Further, in a rat full-thickness wounds model, the ACK sponge containing 10% KFX (ACK-10) significantly facilitates wound closure compared to the AC group and ACK sponge containing 5% and 15% KFX.
33259842	5	23	contain	containing	1141:1150	arg2	KFX					1163:1165	15% KFX	1159:1165	15% KFX	1159:1165	Further, in a rat full-thickness wounds model, the ACK sponge containing 10% KFX (ACK-10) significantly facilitates wound closure compared to the AC group and ACK sponge containing 5% and 15% KFX.
33259842	1	24	with	materials	174:182	arg1	deficiencies					215:226	structural and functional deficiencies	189:226	structural and functional deficiencies	189:226	Conventional wound-dressing materials with structural and functional deficiencies are not effective in promoting wound healing.
33259842	4	25	theme	interconnected	803:816	arg1	transmittance					861:873	suitable water vapor transmittance	840:873	suitable water vapor transmittance	840:873	It is demonstrated that the AG/CMC/KFX (ACK) sponges exhibit a highly interconnected and porous structure, suitable water vapor transmittance, excellent elastic properties, antibacterial behavior, cytocompatibility, and rapid hemostasis.
33259842	4	25	theme	interconnected	803:816	arg1	structure					829:837	a highly interconnected and porous structure	794:837	a highly interconnected and porous structure	794:837	It is demonstrated that the AG/CMC/KFX (ACK) sponges exhibit a highly interconnected and porous structure, suitable water vapor transmittance, excellent elastic properties, antibacterial behavior, cytocompatibility, and rapid hemostasis.
33259842	5	26	contain	containing	1033:1042	arg1	sponge					1026:1031	the ACK sponge	1018:1031	the ACK sponge containing 10% KFX (ACK-10)	1018:1059	Further, in a rat full-thickness wounds model, the ACK sponge containing 10% KFX (ACK-10) significantly facilitates wound closure compared to the AC group and ACK sponge containing 5% and 15% KFX.
33259842	5	26	contain	containing	1033:1042	arg2	ACK-10					1053:1058	ACK-10	1053:1058	ACK-10	1053:1058	Further, in a rat full-thickness wounds model, the ACK sponge containing 10% KFX (ACK-10) significantly facilitates wound closure compared to the AC group and ACK sponge containing 5% and 15% KFX.
33259842	5	26	contain	containing	1033:1042	arg2	KFX					1048:1050	10% KFX	1044:1050	10% KFX (ACK-10)	1044:1059	Further, in a rat full-thickness wounds model, the ACK sponge containing 10% KFX (ACK-10) significantly facilitates wound closure compared to the AC group and ACK sponge containing 5% and 15% KFX.
33259842	5	27	theme	rat	985:987	arg1	model					1011:1015	a rat full-thickness wounds model	983:1015	a rat full-thickness wounds model	983:1015	Further, in a rat full-thickness wounds model, the ACK sponge containing 10% KFX (ACK-10) significantly facilitates wound closure compared to the AC group and ACK sponge containing 5% and 15% KFX.
33259842	4	28	theme	elastic	886:892	arg1	properties					894:903	excellent elastic properties	876:903	excellent elastic properties	876:903	It is demonstrated that the AG/CMC/KFX (ACK) sponges exhibit a highly interconnected and porous structure, suitable water vapor transmittance, excellent elastic properties, antibacterial behavior, cytocompatibility, and rapid hemostasis.
33259842	3	29	theme	green	659:663	arg1	crosslinking					665:676	green crosslinking	659:676	green crosslinking	659:676	Herein, multifunctional composite sponges were developed by incorporation of traditional Chinese medicine Kangfuxin (KFX) into alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods.
33259842	2	30	theme	blood	375:379	arg1	coagulation					381:391	blood coagulation	375:391	blood coagulation	375:391	The development of multifunctional wound dressings is emerging as a promising strategy to accelerate blood coagulation, inhibit bacterial infection, and trigger full-thickness wound into a regenerative process.
33259842	5	31	theme	wound	1087:1091	arg1	closure					1093:1099	wound closure	1087:1099	wound closure	1087:1099	Further, in a rat full-thickness wounds model, the ACK sponge containing 10% KFX (ACK-10) significantly facilitates wound closure compared to the AC group and ACK sponge containing 5% and 15% KFX.
33259842	1	32	theme	wound	259:263	arg1	healing					265:271	wound healing	259:271	wound healing	259:271	Conventional wound-dressing materials with structural and functional deficiencies are not effective in promoting wound healing.
33259842	0	33	theme	antibacterial	16:28	arg1	alginate/carboxymethyl					30:51	A biodegradable antibacterial alginate/carboxymethyl	0:51	A biodegradable antibacterial alginate/carboxymethyl	0:51	A biodegradable antibacterial alginate/carboxymethyl chitosan/Kangfuxin sponges for promoting blood coagulation and full-thickness wound healing.
33259842	4	34	theme	vapor	855:859	arg1	transmittance					861:873	suitable water vapor transmittance	840:873	suitable water vapor transmittance	840:873	It is demonstrated that the AG/CMC/KFX (ACK) sponges exhibit a highly interconnected and porous structure, suitable water vapor transmittance, excellent elastic properties, antibacterial behavior, cytocompatibility, and rapid hemostasis.
33259842	4	34	theme	vapor	855:859	arg1	structure					829:837	a highly interconnected and porous structure	794:837	a highly interconnected and porous structure	794:837	It is demonstrated that the AG/CMC/KFX (ACK) sponges exhibit a highly interconnected and porous structure, suitable water vapor transmittance, excellent elastic properties, antibacterial behavior, cytocompatibility, and rapid hemostasis.
33259842	5	35	theme	ACK	1130:1132	arg1	sponge					1134:1139	ACK sponge	1130:1139	ACK sponge	1130:1139	Further, in a rat full-thickness wounds model, the ACK sponge containing 10% KFX (ACK-10) significantly facilitates wound closure compared to the AC group and ACK sponge containing 5% and 15% KFX.
33259842	1	36	theme	Conventional	146:157	arg1	materials					174:182	Conventional wound-dressing materials	146:182	Conventional wound-dressing materials with structural and functional deficiencies	146:226	Conventional wound-dressing materials with structural and functional deficiencies are not effective in promoting wound healing.
33259842	3	37	theme	traditional	562:572	arg1	medicine					582:589	traditional Chinese medicine Kangfuxin (KFX)	562:605	traditional Chinese medicine Kangfuxin (KFX)	562:605	Herein, multifunctional composite sponges were developed by incorporation of traditional Chinese medicine Kangfuxin (KFX) into alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods.
33259842	2	38	theme	wound	309:313	arg1	dressings					315:323	multifunctional wound dressings	293:323	multifunctional wound dressings	293:323	The development of multifunctional wound dressings is emerging as a promising strategy to accelerate blood coagulation, inhibit bacterial infection, and trigger full-thickness wound into a regenerative process.
33259842	3	39	theme	multifunctional	493:507	arg1	sponges					519:525	multifunctional composite sponges	493:525	multifunctional composite sponges	493:525	Herein, multifunctional composite sponges were developed by incorporation of traditional Chinese medicine Kangfuxin (KFX) into alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods.
33259842	6	40	theme	great	1222:1226	arg1	promise					1228:1234	great promise	1222:1234	great promise for the application of full-thickness wound healing	1222:1286	Thus, the multifunctional ACK-10 composite sponge has great promise for the application of full-thickness wound healing.
33259842	3	41	theme	freeze-drying	710:722	arg1	methods					724:730	freeze-drying methods	710:730	freeze-drying methods	710:730	Herein, multifunctional composite sponges were developed by incorporation of traditional Chinese medicine Kangfuxin (KFX) into alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods.
33259842	2	42	theme	multifunctional	293:307	arg1	dressings					315:323	multifunctional wound dressings	293:323	multifunctional wound dressings	293:323	The development of multifunctional wound dressings is emerging as a promising strategy to accelerate blood coagulation, inhibit bacterial infection, and trigger full-thickness wound into a regenerative process.
33259842	4	43	theme	antibacterial	906:918	arg1	behavior					920:927	antibacterial behavior	906:927	antibacterial behavior	906:927	It is demonstrated that the AG/CMC/KFX (ACK) sponges exhibit a highly interconnected and porous structure, suitable water vapor transmittance, excellent elastic properties, antibacterial behavior, cytocompatibility, and rapid hemostasis.
33259842	5	44	theme	%	1046:1046	arg1	ACK-10					1053:1058	ACK-10	1053:1058	ACK-10	1053:1058	Further, in a rat full-thickness wounds model, the ACK sponge containing 10% KFX (ACK-10) significantly facilitates wound closure compared to the AC group and ACK sponge containing 5% and 15% KFX.
33259842	5	44	theme	%	1046:1046	arg1	KFX					1048:1050	10% KFX	1044:1050	10% KFX (ACK-10)	1044:1059	Further, in a rat full-thickness wounds model, the ACK sponge containing 10% KFX (ACK-10) significantly facilitates wound closure compared to the AC group and ACK sponge containing 5% and 15% KFX.
33259842	1	45	theme	wound-dressing	159:172	arg1	materials					174:182	Conventional wound-dressing materials	146:182	Conventional wound-dressing materials with structural and functional deficiencies	146:226	Conventional wound-dressing materials with structural and functional deficiencies are not effective in promoting wound healing.
33259842	6	46	theme	healing	1280:1286	arg1	application					1244:1254	the application	1240:1254	the application of full-thickness wound healing	1240:1286	Thus, the multifunctional ACK-10 composite sponge has great promise for the application of full-thickness wound healing.
33259842	1	47	theme	functional	204:213	arg1	deficiencies					215:226	structural and functional deficiencies	189:226	structural and functional deficiencies	189:226	Conventional wound-dressing materials with structural and functional deficiencies are not effective in promoting wound healing.
33259842	5	48	theme	wounds	1004:1009	arg1	model					1011:1015	a rat full-thickness wounds model	983:1015	a rat full-thickness wounds model	983:1015	Further, in a rat full-thickness wounds model, the ACK sponge containing 10% KFX (ACK-10) significantly facilitates wound closure compared to the AC group and ACK sponge containing 5% and 15% KFX.
33259842	6	49	theme	full-thickness	1259:1272	arg1	healing					1280:1286	full-thickness wound healing	1259:1286	full-thickness wound healing	1259:1286	Thus, the multifunctional ACK-10 composite sponge has great promise for the application of full-thickness wound healing.
33259842	4	50	theme	excellent	876:884	arg1	properties					894:903	excellent elastic properties	876:903	excellent elastic properties	876:903	It is demonstrated that the AG/CMC/KFX (ACK) sponges exhibit a highly interconnected and porous structure, suitable water vapor transmittance, excellent elastic properties, antibacterial behavior, cytocompatibility, and rapid hemostasis.
33259842	5	51	theme	AC	1117:1118	arg1	group					1120:1124	the AC group	1113:1124	the AC group	1113:1124	Further, in a rat full-thickness wounds model, the ACK sponge containing 10% KFX (ACK-10) significantly facilitates wound closure compared to the AC group and ACK sponge containing 5% and 15% KFX.
33259842	4	52	theme	AG/CMC/KFX	761:770	arg1	sponges					778:784	the AG/CMC/KFX (ACK) sponges	757:784	the AG/CMC/KFX (ACK) sponges	757:784	It is demonstrated that the AG/CMC/KFX (ACK) sponges exhibit a highly interconnected and porous structure, suitable water vapor transmittance, excellent elastic properties, antibacterial behavior, cytocompatibility, and rapid hemostasis.
33259842	2	53	theme	bacterial	402:410	arg1	infection					412:420	bacterial infection	402:420	bacterial infection	402:420	The development of multifunctional wound dressings is emerging as a promising strategy to accelerate blood coagulation, inhibit bacterial infection, and trigger full-thickness wound into a regenerative process.
33259842	6	54	theme	multifunctional	1178:1192	arg1	sponge					1211:1216	the multifunctional ACK-10 composite sponge	1174:1216	the multifunctional ACK-10 composite sponge	1174:1216	Thus, the multifunctional ACK-10 composite sponge has great promise for the application of full-thickness wound healing.
33259842	4	55	theme	water	849:853	arg1	vapor					855:859	suitable water vapor	840:859	suitable water vapor transmittance	840:873	It is demonstrated that the AG/CMC/KFX (ACK) sponges exhibit a highly interconnected and porous structure, suitable water vapor transmittance, excellent elastic properties, antibacterial behavior, cytocompatibility, and rapid hemostasis.
33259842	5	56	theme	10	1044:1045	arg1	%					1046:1046	%	1046:1046	%	1046:1046	Further, in a rat full-thickness wounds model, the ACK sponge containing 10% KFX (ACK-10) significantly facilitates wound closure compared to the AC group and ACK sponge containing 5% and 15% KFX.
33259842	1	57	theme	structural	189:198	arg1	deficiencies					215:226	structural and functional deficiencies	189:226	structural and functional deficiencies	189:226	Conventional wound-dressing materials with structural and functional deficiencies are not effective in promoting wound healing.
33259842	3	58	theme	Chinese	574:580	arg1	medicine					582:589	traditional Chinese medicine Kangfuxin (KFX)	562:605	traditional Chinese medicine Kangfuxin (KFX)	562:605	Herein, multifunctional composite sponges were developed by incorporation of traditional Chinese medicine Kangfuxin (KFX) into alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods.
33259842	3	59	theme	electrostatic	679:691	arg1	interaction					693:703	electrostatic interaction	679:703	electrostatic interaction	679:703	Herein, multifunctional composite sponges were developed by incorporation of traditional Chinese medicine Kangfuxin (KFX) into alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods.
33259842	0	60	theme	blood	94:98	arg1	coagulation					100:110	blood coagulation	94:110	blood coagulation	94:110	A biodegradable antibacterial alginate/carboxymethyl chitosan/Kangfuxin sponges for promoting blood coagulation and full-thickness wound healing.
33259842	2	61	theme	dressings	315:323	arg1	development					278:288	The development	274:288	The development of multifunctional wound dressings	274:323	The development of multifunctional wound dressings is emerging as a promising strategy to accelerate blood coagulation, inhibit bacterial infection, and trigger full-thickness wound into a regenerative process.
33259842	2	62	theme	full-thickness	435:448	arg1	wound					450:454	full-thickness wound	435:454	full-thickness wound into a regenerative process	435:482	The development of multifunctional wound dressings is emerging as a promising strategy to accelerate blood coagulation, inhibit bacterial infection, and trigger full-thickness wound into a regenerative process.
34087301	1	0	theme	singular	140:147	arg1	gel					149:151	a singular gel	138:151	a singular gel	138:151	Compounding is a safe method to avoid limitations of a singular gel.
34087301	8	1	theme	Rheological	977:987	arg1	analysis					989:996	Rheological analysis	977:996	Rheological analysis	977:996	Rheological analysis showed the gels were non-Newtonian pseudoplastic fluids, with anti-thixotropy (GA ≤ 3.5%) and thixotropy (GA ≥ 4.0%).
34087301	7	2	theme	4.0	971:973	arg1	%					966:966	%	966:966	%	966:966	Hardness and springiness dropped when GA concentration increased from 0 to 2.0%, but they increased when GA increased from 2.0% to 4.0%.
34087301	1	3	theme	gel	149:151	arg1	limitations					123:133	limitations	123:133	limitations of a singular gel	123:151	Compounding is a safe method to avoid limitations of a singular gel.
34087301	5	4	contain	contained	727:735	arg1	concentrations					762:775	high GA concentrations	754:775	high GA concentrations	754:775	The microstructures became denser and contained smaller holes at high GA concentrations as seen by SEM.
34087301	5	4	contain	contained	727:735	arg2	microstructures					693:707	The microstructures	689:707	The microstructures	689:707	The microstructures became denser and contained smaller holes at high GA concentrations as seen by SEM.
34087301	5	4	contain	contained	727:735	arg1	microstructures					693:707	The microstructures	689:707	The microstructures	689:707	The microstructures became denser and contained smaller holes at high GA concentrations as seen by SEM.
34087301	5	4	contain	contained	727:735	arg2	holes					745:749	smaller holes	737:749	smaller holes	737:749	The microstructures became denser and contained smaller holes at high GA concentrations as seen by SEM.
34087301	7	5	theme	2.0	963:965	arg1	%					966:966	%	966:966	%	966:966	Hardness and springiness dropped when GA concentration increased from 0 to 2.0%, but they increased when GA increased from 2.0% to 4.0%.
34087301	8	6	dep	showed	998:1003	arg1	fluids					1047:1052	non-Newtonian pseudoplastic fluids	1019:1052	non-Newtonian pseudoplastic fluids	1019:1052	Rheological analysis showed the gels were non-Newtonian pseudoplastic fluids, with anti-thixotropy (GA ≤ 3.5%) and thixotropy (GA ≥ 4.0%).
34087301	2	7	dep	Fourier	256:262	arg1	transform					264:272	transform	264:272	transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis	264:454	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	4	8	theme	hydrogen	580:587	arg1	bonds					589:593	hydrogen bonds	580:593	hydrogen bonds	580:593	FTIR indicated that the interactions of KGM and GA were mainly related to hydrogen bonds and acetyl groups, and the solution separated from gels only included GA and water molecules.
34087301	10	9	theme	non-covalent	1236:1247	arg1	linkages					1249:1256	The non-covalent linkages	1232:1256	The non-covalent linkages	1232:1256	The non-covalent linkages included hydrogen bonding and hydrophobic interaction, and hydrogen bonding held the dominated status.
34087301	4	10	theme	GA	554:555	arg1	related					569:575	related	569:575	related	569:575	FTIR indicated that the interactions of KGM and GA were mainly related to hydrogen bonds and acetyl groups, and the solution separated from gels only included GA and water molecules.
34087301	4	10	theme	GA	554:555	arg1	interactions					530:541	the interactions	526:541	the interactions of KGM and GA	526:555	FTIR indicated that the interactions of KGM and GA were mainly related to hydrogen bonds and acetyl groups, and the solution separated from gels only included GA and water molecules.
34087301	2	11	theme	profile	408:414	arg1	analysis					416:423	texture profile analysis	400:423	texture profile analysis (TPA)	400:429	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	2	11	theme	profile	408:414	arg1	TPA					426:428	TPA	426:428	TPA	426:428	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	8	12	theme	non-Newtonian	1019:1031	arg1	fluids					1047:1052	non-Newtonian pseudoplastic fluids	1019:1052	non-Newtonian pseudoplastic fluids	1019:1052	Rheological analysis showed the gels were non-Newtonian pseudoplastic fluids, with anti-thixotropy (GA ≤ 3.5%) and thixotropy (GA ≥ 4.0%).
34087301	7	13	dep	2.0	915:917	arg1	to					912:913	to	912:913	to	912:913	Hardness and springiness dropped when GA concentration increased from 0 to 2.0%, but they increased when GA increased from 2.0% to 4.0%.
34087301	8	14	theme	pseudoplastic	1033:1045	arg1	fluids					1047:1052	non-Newtonian pseudoplastic fluids	1019:1052	non-Newtonian pseudoplastic fluids	1019:1052	Rheological analysis showed the gels were non-Newtonian pseudoplastic fluids, with anti-thixotropy (GA ≤ 3.5%) and thixotropy (GA ≥ 4.0%).
34087301	2	15	theme	texture	400:406	arg1	analysis					416:423	texture profile analysis	400:423	texture profile analysis (TPA)	400:429	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	2	15	theme	texture	400:406	arg1	TPA					426:428	TPA	426:428	TPA	426:428	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	5	16	theme	high	754:757	arg1	concentrations					762:775	high GA concentrations	754:775	high GA concentrations	754:775	The microstructures became denser and contained smaller holes at high GA concentrations as seen by SEM.
34087301	7	17	dep	%	966:966	arg1	to					968:969	to	968:969	to	968:969	Hardness and springiness dropped when GA concentration increased from 0 to 2.0%, but they increased when GA increased from 2.0% to 4.0%.
34087301	10	18	theme	hydrophobic	1288:1298	arg1	interaction					1300:1310	hydrophobic interaction	1288:1310	hydrophobic interaction	1288:1310	The non-covalent linkages included hydrogen bonding and hydrophobic interaction, and hydrogen bonding held the dominated status.
34087301	11	19	theme	synergistic	1405:1415	arg1	effects					1417:1423	antagonistic and synergistic effects	1388:1423	antagonistic and synergistic effects	1388:1423	Therefore, KGM and GA have antagonistic and synergistic effects.
34087301	5	20	theme	GA	759:760	arg1	concentrations					762:775	high GA concentrations	754:775	high GA concentrations	754:775	The microstructures became denser and contained smaller holes at high GA concentrations as seen by SEM.
34087301	9	21	theme	non-covalent	1161:1172	arg1	gels					1174:1177	non-covalent gels	1161:1177	non-covalent gels	1161:1177	Furthermore, the gels could be classified as non-covalent gels, with higher gel strength at high GA concentrations.
34087301	9	21	theme	non-covalent	1161:1172	arg1	gels					1133:1136	the gels	1129:1136	the gels	1129:1136	Furthermore, the gels could be classified as non-covalent gels, with higher gel strength at high GA concentrations.
34087301	11	22	theme	antagonistic	1388:1399	arg1	effects					1417:1423	antagonistic and synergistic effects	1388:1423	antagonistic and synergistic effects	1388:1423	Therefore, KGM and GA have antagonistic and synergistic effects.
34087301	6	23	theme	increasing	828:837	arg1	GA					825:826	GA increasing	825:837	GA increasing	825:837	WSI and WAI both increased with GA increasing.
34087301	2	24	theme	rheological	435:445	arg1	analysis					447:454	rheological analysis	435:454	rheological analysis	435:454	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	2	25	theme	composite	160:168	arg1	gels					170:173	composite gels	160:173	composite gels	160:173	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	1	26	theme	safe	102:105	arg1	method					107:112	a safe method	100:112	a safe method to avoid limitations of a singular gel	100:151	Compounding is a safe method to avoid limitations of a singular gel.
34087301	1	26	theme	safe	102:105	arg1	Compounding					85:95	Compounding	85:95	Compounding	85:95	Compounding is a safe method to avoid limitations of a singular gel.
34087301	2	27	theme	solubility	346:355	arg1	WSI					364:366	WSI	364:366	WSI	364:366	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	2	27	theme	solubility	346:355	arg1	index					357:361	water solubility index	340:361	water solubility index (WSI)	340:367	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	0	28	theme	glucomannan	43:53	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of konjac glucomannan and gum arabic composite gel.
34087301	0	28	theme	glucomannan	43:53	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of konjac glucomannan and gum arabic composite gel.
34087301	4	29	theme	KGM	546:548	arg1	related					569:575	related	569:575	related	569:575	FTIR indicated that the interactions of KGM and GA were mainly related to hydrogen bonds and acetyl groups, and the solution separated from gels only included GA and water molecules.
34087301	4	29	theme	KGM	546:548	arg1	interactions					530:541	the interactions	526:541	the interactions of KGM and GA	526:555	FTIR indicated that the interactions of KGM and GA were mainly related to hydrogen bonds and acetyl groups, and the solution separated from gels only included GA and water molecules.
34087301	2	30	theme	water	340:344	arg1	WSI					364:366	WSI	364:366	WSI	364:366	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	2	30	theme	water	340:344	arg1	index					357:361	water solubility index	340:361	water solubility index (WSI)	340:367	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	0	31	theme	konjac	36:41	arg1	glucomannan					43:53	konjac glucomannan	36:53	konjac glucomannan	36:53	Preparation and characterization of konjac glucomannan and gum arabic composite gel.
34087301	10	32	theme	hydrogen	1317:1324	arg1	bonding					1326:1332	hydrogen bonding	1317:1332	hydrogen bonding	1317:1332	The non-covalent linkages included hydrogen bonding and hydrophobic interaction, and hydrogen bonding held the dominated status.
34087301	5	33	theme	smaller	737:743	arg1	holes					745:749	smaller holes	737:749	smaller holes	737:749	The microstructures became denser and contained smaller holes at high GA concentrations as seen by SEM.
34087301	0	34	theme	gum	59:61	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of konjac glucomannan and gum arabic composite gel.
34087301	0	34	theme	gum	59:61	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of konjac glucomannan and gum arabic composite gel.
34087301	9	35	theme	higher	1185:1190	arg1	strength					1196:1203	higher gel strength	1185:1203	higher gel strength at high GA concentrations	1185:1229	Furthermore, the gels could be classified as non-covalent gels, with higher gel strength at high GA concentrations.
34087301	4	36	theme	acetyl	599:604	arg1	groups					606:611	acetyl groups	599:611	acetyl groups	599:611	FTIR indicated that the interactions of KGM and GA were mainly related to hydrogen bonds and acetyl groups, and the solution separated from gels only included GA and water molecules.
34087301	2	37	theme	water	370:374	arg1	WAI					394:396	WAI	394:396	WAI	394:396	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	2	37	theme	water	370:374	arg1	index					387:391	water absorption index	370:391	water absorption index (WAI)	370:397	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	9	38	theme	gel	1192:1194	arg1	strength					1196:1203	higher gel strength	1185:1203	higher gel strength at high GA concentrations	1185:1229	Furthermore, the gels could be classified as non-covalent gels, with higher gel strength at high GA concentrations.
34087301	9	39	from	concentrations	1216:1229	arg1	strength					1196:1203	higher gel strength	1185:1203	higher gel strength at high GA concentrations	1185:1229	Furthermore, the gels could be classified as non-covalent gels, with higher gel strength at high GA concentrations.
34087301	2	40	theme	konjac	194:199	arg1	KGM					214:216	KGM	214:216	KGM	214:216	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	2	40	theme	konjac	194:199	arg1	glucomannan					201:211	konjac glucomannan	194:211	konjac glucomannan (KGM)	194:217	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	7	41	theme	GA	878:879	arg1	concentration					881:893	GA concentration	878:893	GA concentration	878:893	Hardness and springiness dropped when GA concentration increased from 0 to 2.0%, but they increased when GA increased from 2.0% to 4.0%.
34087301	10	42	theme	hydrogen	1267:1274	arg1	bonding					1276:1282	hydrogen bonding	1267:1282	hydrogen bonding	1267:1282	The non-covalent linkages included hydrogen bonding and hydrophobic interaction, and hydrogen bonding held the dominated status.
34087301	11	43	contain	have	1383:1386	arg2	effects					1417:1423	antagonistic and synergistic effects	1388:1423	antagonistic and synergistic effects	1388:1423	Therefore, KGM and GA have antagonistic and synergistic effects.
34087301	11	43	contain	have	1383:1386	arg1	GA					1380:1381	GA	1380:1381	GA	1380:1381	Therefore, KGM and GA have antagonistic and synergistic effects.
34087301	11	43	contain	have	1383:1386	arg1	KGM					1372:1374	KGM	1372:1374	KGM	1372:1374	Therefore, KGM and GA have antagonistic and synergistic effects.
34087301	10	44	theme	dominated	1343:1351	arg1	status					1353:1358	the dominated status	1339:1358	the dominated status	1339:1358	The non-covalent linkages included hydrogen bonding and hydrophobic interaction, and hydrogen bonding held the dominated status.
34087301	2	45	theme	gum	223:225	arg1	GA					235:236	GA	235:236	GA	235:236	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	2	45	theme	gum	223:225	arg1	arabic					227:232	gum arabic	223:232	gum arabic (GA)	223:237	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	2	46	theme	infrared	274:281	arg1	FTIR					297:300	FTIR	297:300	FTIR	297:300	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	2	46	theme	infrared	274:281	arg1	spectroscopy					283:294	infrared spectroscopy	274:294	infrared spectroscopy (FTIR)	274:301	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	9	47	theme	high	1208:1211	arg1	concentrations					1216:1229	high GA concentrations	1208:1229	high GA concentrations	1208:1229	Furthermore, the gels could be classified as non-covalent gels, with higher gel strength at high GA concentrations.
34087301	0	48	theme	composite	70:78	arg1	gel					80:82	composite gel	70:82	composite gel	70:82	Preparation and characterization of konjac glucomannan and gum arabic composite gel.
34087301	2	49	theme	electron	313:320	arg1	SEM					334:336	SEM	334:336	SEM	334:336	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	2	49	theme	electron	313:320	arg1	microscopy					322:331	scanning electron microscopy	304:331	scanning electron microscopy (SEM)	304:337	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	9	50	theme	GA	1213:1214	arg1	concentrations					1216:1229	high GA concentrations	1208:1229	high GA concentrations	1208:1229	Furthermore, the gels could be classified as non-covalent gels, with higher gel strength at high GA concentrations.
34087301	2	51	theme	absorption	376:385	arg1	WAI					394:396	WAI	394:396	WAI	394:396	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	2	51	theme	absorption	376:385	arg1	index					387:391	water absorption index	370:391	water absorption index (WAI)	370:397	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	4	52	theme	water	672:676	arg1	molecules					678:686	water molecules	672:686	water molecules	672:686	FTIR indicated that the interactions of KGM and GA were mainly related to hydrogen bonds and acetyl groups, and the solution separated from gels only included GA and water molecules.
34087301	2	53	theme	scanning	304:311	arg1	SEM					334:336	SEM	334:336	SEM	334:336	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
34087301	2	53	theme	scanning	304:311	arg1	microscopy					322:331	scanning electron microscopy	304:331	scanning electron microscopy (SEM)	304:337	Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis.
32946219	5	0	theme	complex	1204:1210	arg1	systems					1222:1228	animal-derived or complex synthetic systems	1186:1228	animal-derived or complex synthetic systems	1186:1228	The work therefore shows that MC-HA hydrogels demonstrate potential for in vitro 3D cell culture as inexpensive and well-defined alternatives to animal-derived or complex synthetic systems.
32946219	2	1	theme	components	381:390	arg1	compatibility					355:367	the biological compatibility	340:367	the biological compatibility of hydrogel components and optimization of the cell culture environment	340:439	Initial work focused on the preparation of hydrogels for 3D culture, followed by investigations of the biological compatibility of hydrogel components and optimization of the cell culture environment.
32946219	4	2	theme	tissue	1019:1024	arg1	tests					1034:1038	in vitro tissue culture tests	1010:1038	in vitro tissue culture tests	1010:1038	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	4	3	dep	released/recovered	801:818	arg1	i					763:763	i	763:763	i	763:763	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	4	4	theme	plate	962:966	arg1	format					968:973	a 96-well plate format	952:973	a 96-well plate format	952:973	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	5	5	theme	in	1113:1114	arg1	culture					1130:1136	in vitro 3D cell culture	1113:1136	in vitro 3D cell culture	1113:1136	The work therefore shows that MC-HA hydrogels demonstrate potential for in vitro 3D cell culture as inexpensive and well-defined alternatives to animal-derived or complex synthetic systems.
32946219	2	6	theme	hydrogel	372:379	arg1	components					381:390	hydrogel components	372:390	hydrogel components	372:390	Initial work focused on the preparation of hydrogels for 3D culture, followed by investigations of the biological compatibility of hydrogel components and optimization of the cell culture environment.
32946219	4	7	from	application	937:947	arg1	format					968:973	a 96-well plate format	952:973	a 96-well plate format	952:973	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	3	8	theme	cells	494:498	arg1	proliferation					470:482	proliferation	470:482	proliferation	470:482	Evaluation of viability and proliferation of HCT116 cells cultured in the MC-HA hydrogel was used to adjust the blend composition to design a hydrogel with optimal properties to support cell growth.
32946219	3	8	theme	cells	494:498	arg1	viability					456:464	viability	456:464	viability	456:464	Evaluation of viability and proliferation of HCT116 cells cultured in the MC-HA hydrogel was used to adjust the blend composition to design a hydrogel with optimal properties to support cell growth.
32946219	3	9	used	used	535:538	arg2	Evaluation					442:451	Evaluation	442:451	Evaluation of viability and proliferation of HCT116 cells cultured in the MC-HA hydrogel	442:529	Evaluation of viability and proliferation of HCT116 cells cultured in the MC-HA hydrogel was used to adjust the blend composition to design a hydrogel with optimal properties to support cell growth.
32946219	4	10	theme	3D	766:767	arg1	aggregates					783:792	3D cultured cell aggregates	766:792	3D cultured cell aggregates	766:792	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	4	11	theme	application	679:689	arg1	terms					666:670	terms	666:670	terms of the application of the proposed polymeric matrix in 3D cell culture	666:741	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	4	12	dep	matrix	915:920	arg1	ii					898:899	ii	898:899	ii	898:899	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	4	12	dep	matrix	915:920	arg1	amenable					925:932	amenable	925:932	amenable	925:932	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	4	13	theme	cell	878:881	arg1	viability					883:891	cell viability	878:891	cell viability	878:891	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	3	14	theme	MC-HA	516:520	arg1	hydrogel					522:529	the MC-HA hydrogel	512:529	the MC-HA hydrogel	512:529	Evaluation of viability and proliferation of HCT116 cells cultured in the MC-HA hydrogel was used to adjust the blend composition to design a hydrogel with optimal properties to support cell growth.
32946219	4	15	theme	matrix	717:722	arg1	application					679:689	the application	675:689	the application of the proposed polymeric matrix in 3D cell culture	675:741	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	5	16	theme	MC-HA	1071:1075	arg1	hydrogels					1077:1085	MC-HA hydrogels	1071:1085	MC-HA hydrogels	1071:1085	The work therefore shows that MC-HA hydrogels demonstrate potential for in vitro 3D cell culture as inexpensive and well-defined alternatives to animal-derived or complex synthetic systems.
32946219	4	17	theme	gentle	842:847	arg1	procedure					849:857	a gentle procedure	840:857	a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests	840:1038	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	5	18	link	animal-derived	1186:1199	arg1	systems					1222:1228	animal-derived or complex synthetic systems	1186:1228	animal-derived or complex synthetic systems	1186:1228	The work therefore shows that MC-HA hydrogels demonstrate potential for in vitro 3D cell culture as inexpensive and well-defined alternatives to animal-derived or complex synthetic systems.
32946219	2	19	theme	3D	298:299	arg1	culture					301:307	3D culture	298:307	3D culture	298:307	Initial work focused on the preparation of hydrogels for 3D culture, followed by investigations of the biological compatibility of hydrogel components and optimization of the cell culture environment.
32946219	3	20	theme	viability	456:464	arg1	Evaluation					442:451	Evaluation	442:451	Evaluation of viability and proliferation of HCT116 cells cultured in the MC-HA hydrogel	442:529	Evaluation of viability and proliferation of HCT116 cells cultured in the MC-HA hydrogel was used to adjust the blend composition to design a hydrogel with optimal properties to support cell growth.
32946219	2	21	theme	culture	421:427	arg1	environment					429:439	the cell culture environment	412:439	the cell culture environment	412:439	Initial work focused on the preparation of hydrogels for 3D culture, followed by investigations of the biological compatibility of hydrogel components and optimization of the cell culture environment.
32946219	4	22	theme	standard	980:987	arg1	approach					989:996	a standard approach	978:996	a standard approach employed in in vitro tissue culture tests	978:1038	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	4	23	dep	in	1010:1011	arg1	vitro					1013:1017	vitro	1013:1017	vitro	1013:1017	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	3	24	with	hydrogel	584:591	arg1	properties					606:615	optimal properties	598:615	optimal properties	598:615	Evaluation of viability and proliferation of HCT116 cells cultured in the MC-HA hydrogel was used to adjust the blend composition to design a hydrogel with optimal properties to support cell growth.
32946219	4	25	from	application	679:689	arg1	culture					735:741	3D cell culture	727:741	3D cell culture	727:741	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	2	26	theme	cell	416:419	arg1	environment					429:439	the cell culture environment	412:439	the cell culture environment	412:439	Initial work focused on the preparation of hydrogels for 3D culture, followed by investigations of the biological compatibility of hydrogel components and optimization of the cell culture environment.
32946219	0	27	theme	Methylcellulose-Hyaluronic	23:48	arg1	Hydrogel					50:57	Temperature-Responsive Methylcellulose-Hyaluronic Hydrogel	0:57	Temperature-Responsive Methylcellulose-Hyaluronic Hydrogel	0:57	Temperature-Responsive Methylcellulose-Hyaluronic Hydrogel as a 3D Cell Culture Matrix.
32946219	5	28	dep	in	1113:1114	arg1	vitro					1116:1120	vitro	1116:1120	vitro	1116:1120	The work therefore shows that MC-HA hydrogels demonstrate potential for in vitro 3D cell culture as inexpensive and well-defined alternatives to animal-derived or complex synthetic systems.
32946219	1	29	theme	3D	216:217	arg1	growth					224:229	3D cell growth	216:229	3D cell growth	216:229	This study investigated the application of a temperature-responsive methylcellulose-hyaluronic acid (MC-HA) hydrogel to support 3D cell growth in vitro.
32946219	0	30	theme	Temperature-Responsive	0:21	arg1	Hydrogel					50:57	Temperature-Responsive Methylcellulose-Hyaluronic Hydrogel	0:57	Temperature-Responsive Methylcellulose-Hyaluronic Hydrogel	0:57	Temperature-Responsive Methylcellulose-Hyaluronic Hydrogel as a 3D Cell Culture Matrix.
32946219	5	31	theme	inexpensive	1141:1151	arg1	alternatives					1170:1181	inexpensive and well-defined alternatives	1141:1181	inexpensive and well-defined alternatives to animal-derived or complex synthetic systems	1141:1228	The work therefore shows that MC-HA hydrogels demonstrate potential for in vitro 3D cell culture as inexpensive and well-defined alternatives to animal-derived or complex synthetic systems.
32946219	3	32	theme	optimal	598:604	arg1	properties					606:615	optimal properties	598:615	optimal properties	598:615	Evaluation of viability and proliferation of HCT116 cells cultured in the MC-HA hydrogel was used to adjust the blend composition to design a hydrogel with optimal properties to support cell growth.
32946219	1	33	theme	cell	219:222	arg1	growth					224:229	3D cell growth	216:229	3D cell growth	216:229	This study investigated the application of a temperature-responsive methylcellulose-hyaluronic acid (MC-HA) hydrogel to support 3D cell growth in vitro.
32946219	4	34	theme	cultured	769:776	arg1	aggregates					783:792	3D cultured cell aggregates	766:792	3D cultured cell aggregates	766:792	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	4	35	theme	hydrogel	906:913	arg1	matrix					915:920	the hydrogel matrix	902:920	(ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests	897:1038	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	4	36	from	terms	666:670	arg1	culture					735:741	3D cell culture	727:741	3D cell culture	727:741	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	4	37	theme	in	1010:1011	arg1	tests					1034:1038	in vitro tissue culture tests	1010:1038	in vitro tissue culture tests	1010:1038	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	5	38	theme	well-defined	1157:1168	arg1	alternatives					1170:1181	inexpensive and well-defined alternatives	1141:1181	inexpensive and well-defined alternatives to animal-derived or complex synthetic systems	1141:1228	The work therefore shows that MC-HA hydrogels demonstrate potential for in vitro 3D cell culture as inexpensive and well-defined alternatives to animal-derived or complex synthetic systems.
32946219	3	39	theme	blend	554:558	arg1	composition					560:570	the blend composition	550:570	the blend composition to design a hydrogel with optimal properties to support cell growth	550:638	Evaluation of viability and proliferation of HCT116 cells cultured in the MC-HA hydrogel was used to adjust the blend composition to design a hydrogel with optimal properties to support cell growth.
32946219	1	40	theme	hydrogel	196:203	arg1	application					116:126	the application	112:126	the application of a temperature-responsive methylcellulose-hyaluronic acid (MC-HA) hydrogel to support 3D cell growth in vitro	112:238	This study investigated the application of a temperature-responsive methylcellulose-hyaluronic acid (MC-HA) hydrogel to support 3D cell growth in vitro.
32946219	4	41	theme	polymeric	707:715	arg1	matrix					717:722	the proposed polymeric matrix	694:722	the proposed polymeric matrix	694:722	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	0	42	theme	3D	64:65	arg1	Matrix					80:85	a 3D Cell Culture Matrix	62:85	a 3D Cell Culture Matrix	62:85	Temperature-Responsive Methylcellulose-Hyaluronic Hydrogel as a 3D Cell Culture Matrix.
32946219	2	43	theme	compatibility	355:367	arg1	investigations					322:335	investigations	322:335	investigations of the biological compatibility of hydrogel components and optimization of the cell culture environment	322:439	Initial work focused on the preparation of hydrogels for 3D culture, followed by investigations of the biological compatibility of hydrogel components and optimization of the cell culture environment.
32946219	4	44	from	aspects	655:661	arg1	terms					666:670	terms	666:670	terms of the application of the proposed polymeric matrix in 3D cell culture	666:741	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	3	45	theme	HCT116	487:492	arg1	cells					494:498	HCT116 cells	487:498	HCT116 cells cultured in the MC-HA hydrogel	487:529	Evaluation of viability and proliferation of HCT116 cells cultured in the MC-HA hydrogel was used to adjust the blend composition to design a hydrogel with optimal properties to support cell growth.
32946219	3	46	theme	proliferation	470:482	arg1	Evaluation					442:451	Evaluation	442:451	Evaluation of viability and proliferation of HCT116 cells cultured in the MC-HA hydrogel	442:529	Evaluation of viability and proliferation of HCT116 cells cultured in the MC-HA hydrogel was used to adjust the blend composition to design a hydrogel with optimal properties to support cell growth.
32946219	5	47	theme	3D	1122:1123	arg1	culture					1130:1136	in vitro 3D cell culture	1113:1136	in vitro 3D cell culture	1113:1136	The work therefore shows that MC-HA hydrogels demonstrate potential for in vitro 3D cell culture as inexpensive and well-defined alternatives to animal-derived or complex synthetic systems.
32946219	5	48	theme	animal-derived	1186:1199	arg1	systems					1222:1228	animal-derived or complex synthetic systems	1186:1228	animal-derived or complex synthetic systems	1186:1228	The work therefore shows that MC-HA hydrogels demonstrate potential for in vitro 3D cell culture as inexpensive and well-defined alternatives to animal-derived or complex synthetic systems.
32946219	3	49	theme	cell	628:631	arg1	growth					633:638	cell growth	628:638	cell growth	628:638	Evaluation of viability and proliferation of HCT116 cells cultured in the MC-HA hydrogel was used to adjust the blend composition to design a hydrogel with optimal properties to support cell growth.
32946219	2	50	theme	environment	429:439	arg1	components					381:390	hydrogel components	372:390	hydrogel components	372:390	Initial work focused on the preparation of hydrogels for 3D culture, followed by investigations of the biological compatibility of hydrogel components and optimization of the cell culture environment.
32946219	2	50	theme	environment	429:439	arg1	optimization					396:407	optimization	396:407	optimization of the cell culture environment	396:439	Initial work focused on the preparation of hydrogels for 3D culture, followed by investigations of the biological compatibility of hydrogel components and optimization of the cell culture environment.
32946219	0	51	theme	Culture	72:78	arg1	Matrix					80:85	a 3D Cell Culture Matrix	62:85	a 3D Cell Culture Matrix	62:85	Temperature-Responsive Methylcellulose-Hyaluronic Hydrogel as a 3D Cell Culture Matrix.
32946219	5	52	theme	cell	1125:1128	arg1	culture					1130:1136	in vitro 3D cell culture	1113:1136	in vitro 3D cell culture	1113:1136	The work therefore shows that MC-HA hydrogels demonstrate potential for in vitro 3D cell culture as inexpensive and well-defined alternatives to animal-derived or complex synthetic systems.
32946219	4	53	theme	cell	730:733	arg1	culture					735:741	3D cell culture	727:741	3D cell culture	727:741	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	0	54	theme	Cell	67:70	arg1	Matrix					80:85	a 3D Cell Culture Matrix	62:85	a 3D Cell Culture Matrix	62:85	Temperature-Responsive Methylcellulose-Hyaluronic Hydrogel as a 3D Cell Culture Matrix.
32946219	2	55	theme	Initial	241:247	arg1	work					249:252	Initial work	241:252	Initial work	241:252	Initial work focused on the preparation of hydrogels for 3D culture, followed by investigations of the biological compatibility of hydrogel components and optimization of the cell culture environment.
32946219	1	56	theme	temperature-responsive	133:154	arg1	hydrogel					196:203	a temperature-responsive methylcellulose-hyaluronic acid (MC-HA) hydrogel	131:203	a temperature-responsive methylcellulose-hyaluronic acid (MC-HA) hydrogel	131:203	This study investigated the application of a temperature-responsive methylcellulose-hyaluronic acid (MC-HA) hydrogel to support 3D cell growth in vitro.
32946219	2	57	theme	hydrogels	284:292	arg1	preparation					269:279	the preparation	265:279	the preparation of hydrogels for 3D culture	265:307	Initial work focused on the preparation of hydrogels for 3D culture, followed by investigations of the biological compatibility of hydrogel components and optimization of the cell culture environment.
32946219	4	58	theme	proposed	698:705	arg1	matrix					717:722	the proposed polymeric matrix	694:722	the proposed polymeric matrix	694:722	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	1	59	theme	methylcellulose-hyaluronic	156:181	arg1	hydrogel					196:203	a temperature-responsive methylcellulose-hyaluronic acid (MC-HA) hydrogel	131:203	a temperature-responsive methylcellulose-hyaluronic acid (MC-HA) hydrogel	131:203	This study investigated the application of a temperature-responsive methylcellulose-hyaluronic acid (MC-HA) hydrogel to support 3D cell growth in vitro.
32946219	4	60	from	culture	735:741	arg1	terms					666:670	terms	666:670	terms of the application of the proposed polymeric matrix in 3D cell culture	666:741	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	5	61	theme	synthetic	1212:1220	arg1	systems					1222:1228	animal-derived or complex synthetic systems	1186:1228	animal-derived or complex synthetic systems	1186:1228	The work therefore shows that MC-HA hydrogels demonstrate potential for in vitro 3D cell culture as inexpensive and well-defined alternatives to animal-derived or complex synthetic systems.
32946219	4	62	theme	cell	778:781	arg1	aggregates					783:792	3D cultured cell aggregates	766:792	3D cultured cell aggregates	766:792	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	0	63	dep	Hydrogel	50:57	arg1	Matrix					80:85	a 3D Cell Culture Matrix	62:85	a 3D Cell Culture Matrix	62:85	Temperature-Responsive Methylcellulose-Hyaluronic Hydrogel as a 3D Cell Culture Matrix.
32946219	1	64	theme	acid	183:186	arg1	hydrogel					196:203	a temperature-responsive methylcellulose-hyaluronic acid (MC-HA) hydrogel	131:203	a temperature-responsive methylcellulose-hyaluronic acid (MC-HA) hydrogel	131:203	This study investigated the application of a temperature-responsive methylcellulose-hyaluronic acid (MC-HA) hydrogel to support 3D cell growth in vitro.
32946219	2	65	theme	biological	344:353	arg1	compatibility					355:367	the biological compatibility	340:367	the biological compatibility of hydrogel components and optimization of the cell culture environment	340:439	Initial work focused on the preparation of hydrogels for 3D culture, followed by investigations of the biological compatibility of hydrogel components and optimization of the cell culture environment.
32946219	4	66	theme	96-well	954:960	arg1	format					968:973	a 96-well plate format	952:973	a 96-well plate format	952:973	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	2	67	dep	focused	254:260	arg1	followed					310:317	followed	310:317	followed by investigations of the biological compatibility of hydrogel components and optimization of the cell culture environment	310:439	Initial work focused on the preparation of hydrogels for 3D culture, followed by investigations of the biological compatibility of hydrogel components and optimization of the cell culture environment.
32946219	2	68	theme	optimization	396:407	arg1	compatibility					355:367	the biological compatibility	340:367	the biological compatibility of hydrogel components and optimization of the cell culture environment	340:439	Initial work focused on the preparation of hydrogels for 3D culture, followed by investigations of the biological compatibility of hydrogel components and optimization of the cell culture environment.
32946219	4	69	theme	important	645:653	arg1	aspects					655:661	Two important aspects	641:661	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture	641:741	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	1	70	theme	MC-HA	189:193	arg1	hydrogel					196:203	a temperature-responsive methylcellulose-hyaluronic acid (MC-HA) hydrogel	131:203	a temperature-responsive methylcellulose-hyaluronic acid (MC-HA) hydrogel	131:203	This study investigated the application of a temperature-responsive methylcellulose-hyaluronic acid (MC-HA) hydrogel to support 3D cell growth in vitro.
32946219	4	71	theme	culture	1026:1032	arg1	tests					1034:1038	in vitro tissue culture tests	1010:1038	in vitro tissue culture tests	1010:1038	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
32946219	4	72	theme	3D	727:728	arg1	culture					735:741	3D cell culture	727:741	3D cell culture	727:741	Two important aspects in terms of the application of the proposed polymeric matrix in 3D cell culture were demonstrated: (i) 3D cultured cell aggregates can be released/recovered from the matrix via a gentle procedure that will preserve cell viability and (ii) the hydrogel matrix is amenable to application in a 96-well plate format as a standard approach employed in in vitro tissue culture tests.
34444931	3	0	contain	has	405:407	arg2	popularity					423:432	increased its popularity	409:432	increased its popularity in cuisine and traditional medicine	409:468	More recently, black garlic (BG), made by heat-ageing white garlic bulbs, has increased its popularity in cuisine and traditional medicine around the world, but there is still limited information on its composition and potential beneficial effects.
34444931	3	0	contain	has	405:407	arg1	BG					360:361	BG	360:361	BG	360:361	More recently, black garlic (BG), made by heat-ageing white garlic bulbs, has increased its popularity in cuisine and traditional medicine around the world, but there is still limited information on its composition and potential beneficial effects.
34444931	3	0	contain	has	405:407	arg1	garlic					352:357	black garlic	346:357	black garlic (BG)	346:362	More recently, black garlic (BG), made by heat-ageing white garlic bulbs, has increased its popularity in cuisine and traditional medicine around the world, but there is still limited information on its composition and potential beneficial effects.
34444931	5	1	theme	BGE	802:804	arg1	components					812:821	BGE major components	802:821	BGE major components	802:821	Results allowed to establish that BGE major components were sulfur derivatives, saccharides, peptides, organic acids, a phenylpropanoid derivative, saponins, and compounds typical of glycerophospholipid metabolism.
34444931	5	2	theme	glycerophospholipid	951:969	arg1	metabolism					971:980	glycerophospholipid metabolism	951:980	glycerophospholipid metabolism	951:980	Results allowed to establish that BGE major components were sulfur derivatives, saccharides, peptides, organic acids, a phenylpropanoid derivative, saponins, and compounds typical of glycerophospholipid metabolism.
34444931	6	3	from	Characterization	983:998	arg1	cells					1028:1032	cancer cells	1021:1032	cancer cells	1021:1032	Characterization of the BGE action in cancer cells revealed that antioxidant, metabolic, and hepatoprotective effects occur upon treatment as well as induction of maturation of acute myeloid leukemia cells.
34444931	3	4	theme	increased	409:417	arg1	popularity					423:432	increased its popularity	409:432	increased its popularity in cuisine and traditional medicine	409:468	More recently, black garlic (BG), made by heat-ageing white garlic bulbs, has increased its popularity in cuisine and traditional medicine around the world, but there is still limited information on its composition and potential beneficial effects.
34444931	6	5	theme	action	1011:1016	arg1	Characterization					983:998	Characterization	983:998	Characterization of the BGE action in cancer cells	983:1032	Characterization of the BGE action in cancer cells revealed that antioxidant, metabolic, and hepatoprotective effects occur upon treatment as well as induction of maturation of acute myeloid leukemia cells.
34444931	5	6	theme	phenylpropanoid	888:902	arg1	derivative					904:913	a phenylpropanoid derivative	886:913	a phenylpropanoid derivative	886:913	Results allowed to establish that BGE major components were sulfur derivatives, saccharides, peptides, organic acids, a phenylpropanoid derivative, saponins, and compounds typical of glycerophospholipid metabolism.
34444931	7	7	theme	functional	1273:1282	arg1	food					1284:1287	a functional food	1271:1287	a functional food for potential prevention of metabolic and tumor diseases	1271:1344	These results are interesting from the impact point of view of BG consumption as a functional food for potential prevention of metabolic and tumor diseases.
34444931	7	8	theme	impact	1229:1234	arg1	point					1236:1240	the impact point	1225:1240	the impact point of view of BG consumption	1225:1266	These results are interesting from the impact point of view of BG consumption as a functional food for potential prevention of metabolic and tumor diseases.
34444931	7	9	theme	metabolic	1317:1325	arg1	diseases					1337:1344	metabolic and tumor diseases	1317:1344	metabolic and tumor diseases	1317:1344	These results are interesting from the impact point of view of BG consumption as a functional food for potential prevention of metabolic and tumor diseases.
34444931	5	10	theme	organic	871:877	arg1	acids					879:883	organic acids	871:883	organic acids	871:883	Results allowed to establish that BGE major components were sulfur derivatives, saccharides, peptides, organic acids, a phenylpropanoid derivative, saponins, and compounds typical of glycerophospholipid metabolism.
34444931	3	11	theme	traditional	449:459	arg1	medicine					461:468	traditional medicine	449:468	traditional medicine	449:468	More recently, black garlic (BG), made by heat-ageing white garlic bulbs, has increased its popularity in cuisine and traditional medicine around the world, but there is still limited information on its composition and potential beneficial effects.
34444931	0	12	theme	Polar	87:91	arg1	Extract					93:99	Black Garlic (Allium sativum L.) Polar Extract	54:99	Black Garlic (Allium sativum L.) Polar Extract	54:99	Metabolite Profile and In Vitro Beneficial Effects of Black Garlic (Allium sativum L.) Polar Extract.
34444931	6	13	theme	cells	1183:1187	arg1	maturation					1146:1155	maturation	1146:1155	maturation of acute myeloid leukemia cells	1146:1187	Characterization of the BGE action in cancer cells revealed that antioxidant, metabolic, and hepatoprotective effects occur upon treatment as well as induction of maturation of acute myeloid leukemia cells.
34444931	3	14	from	popularity	423:432	arg1	cuisine					437:443	cuisine	437:443	cuisine	437:443	More recently, black garlic (BG), made by heat-ageing white garlic bulbs, has increased its popularity in cuisine and traditional medicine around the world, but there is still limited information on its composition and potential beneficial effects.
34444931	3	14	from	popularity	423:432	arg1	medicine					461:468	traditional medicine	449:468	traditional medicine	449:468	More recently, black garlic (BG), made by heat-ageing white garlic bulbs, has increased its popularity in cuisine and traditional medicine around the world, but there is still limited information on its composition and potential beneficial effects.
34444931	7	15	theme	tumor	1331:1335	arg1	diseases					1337:1344	metabolic and tumor diseases	1317:1344	metabolic and tumor diseases	1317:1344	These results are interesting from the impact point of view of BG consumption as a functional food for potential prevention of metabolic and tumor diseases.
34444931	5	16	theme	sulfur	828:833	arg1	derivatives					835:845	sulfur derivatives	828:845	sulfur derivatives	828:845	Results allowed to establish that BGE major components were sulfur derivatives, saccharides, peptides, organic acids, a phenylpropanoid derivative, saponins, and compounds typical of glycerophospholipid metabolism.
34444931	6	17	theme	acute	1160:1164	arg1	leukemia					1174:1181	acute myeloid leukemia	1160:1181	acute myeloid leukemia cells	1160:1187	Characterization of the BGE action in cancer cells revealed that antioxidant, metabolic, and hepatoprotective effects occur upon treatment as well as induction of maturation of acute myeloid leukemia cells.
34444931	5	18	theme	major	806:810	arg1	components					812:821	BGE major components	802:821	BGE major components	802:821	Results allowed to establish that BGE major components were sulfur derivatives, saccharides, peptides, organic acids, a phenylpropanoid derivative, saponins, and compounds typical of glycerophospholipid metabolism.
34444931	6	19	theme	leukemia	1174:1181	arg1	cells					1183:1187	acute myeloid leukemia cells	1160:1187	acute myeloid leukemia cells	1160:1187	Characterization of the BGE action in cancer cells revealed that antioxidant, metabolic, and hepatoprotective effects occur upon treatment as well as induction of maturation of acute myeloid leukemia cells.
34444931	6	20	theme	hepatoprotective	1076:1091	arg1	effects					1093:1099	antioxidant, metabolic, and hepatoprotective effects	1048:1099	antioxidant, metabolic, and hepatoprotective effects	1048:1099	Characterization of the BGE action in cancer cells revealed that antioxidant, metabolic, and hepatoprotective effects occur upon treatment as well as induction of maturation of acute myeloid leukemia cells.
34444931	3	21	from	information	515:525	arg1	effects					571:577	potential beneficial effects	550:577	potential beneficial effects	550:577	More recently, black garlic (BG), made by heat-ageing white garlic bulbs, has increased its popularity in cuisine and traditional medicine around the world, but there is still limited information on its composition and potential beneficial effects.
34444931	3	21	from	information	515:525	arg1	composition					534:544	its composition	530:544	its composition	530:544	More recently, black garlic (BG), made by heat-ageing white garlic bulbs, has increased its popularity in cuisine and traditional medicine around the world, but there is still limited information on its composition and potential beneficial effects.
34444931	2	22	theme	bulbs	324:328	arg1	effects					306:312	the therapeutic effects	290:312	the therapeutic effects of garlic bulbs	290:328	To confirm this, many extensive studies recognized the therapeutic effects of garlic bulbs.
34444931	4	23	from	spectrometry	730:741	arg1	mode					762:765	high-resolution mode	746:765	high-resolution mode	746:765	In this study, the metabolite profile of methanol extract of BG (BGE) was determined by high-performance liquid chromatography coupled to tandem mass spectrometry in high-resolution mode.
34444931	6	24	theme	myeloid	1166:1172	arg1	leukemia					1174:1181	acute myeloid leukemia	1160:1181	acute myeloid leukemia cells	1160:1187	Characterization of the BGE action in cancer cells revealed that antioxidant, metabolic, and hepatoprotective effects occur upon treatment as well as induction of maturation of acute myeloid leukemia cells.
34444931	0	25	dep	Allium	68:73	arg1	L.					83:84	Allium sativum L.	68:84	Allium sativum L.	68:84	Metabolite Profile and In Vitro Beneficial Effects of Black Garlic (Allium sativum L.) Polar Extract.
34444931	6	26	theme	BGE	1007:1009	arg1	action					1011:1016	the BGE action	1003:1016	the BGE action in cancer cells	1003:1032	Characterization of the BGE action in cancer cells revealed that antioxidant, metabolic, and hepatoprotective effects occur upon treatment as well as induction of maturation of acute myeloid leukemia cells.
34444931	2	27	theme	garlic	317:322	arg1	bulbs					324:328	garlic bulbs	317:328	garlic bulbs	317:328	To confirm this, many extensive studies recognized the therapeutic effects of garlic bulbs.
34444931	0	28	theme	Metabolite	0:9	arg1	Profile					11:17	Metabolite Profile	0:17	Metabolite Profile	0:17	Metabolite Profile and In Vitro Beneficial Effects of Black Garlic (Allium sativum L.) Polar Extract.
34444931	4	29	theme	high-resolution	746:760	arg1	mode					762:765	high-resolution mode	746:765	high-resolution mode	746:765	In this study, the metabolite profile of methanol extract of BG (BGE) was determined by high-performance liquid chromatography coupled to tandem mass spectrometry in high-resolution mode.
34444931	4	30	theme	extract	630:636	arg1	profile					610:616	the metabolite profile	595:616	the metabolite profile of methanol extract of BG (BGE)	595:648	In this study, the metabolite profile of methanol extract of BG (BGE) was determined by high-performance liquid chromatography coupled to tandem mass spectrometry in high-resolution mode.
34444931	0	31	theme	In	23:24	arg1	Effects					43:49	In Vitro Beneficial Effects	23:49	In Vitro Beneficial Effects of Black Garlic (Allium sativum L.) Polar Extract	23:99	Metabolite Profile and In Vitro Beneficial Effects of Black Garlic (Allium sativum L.) Polar Extract.
34444931	1	32	dep	Allium	161:166	arg1	L.					176:177	Allium sativum L.	161:177	Allium sativum L.	161:177	Over the centuries, humans have traditionally used garlic (Allium sativum L.) as a food ingredient (spice) and remedy for many diseases.
34444931	7	33	theme	consumption	1256:1266	arg1	view					1245:1248	view	1245:1248	view of BG consumption	1245:1266	These results are interesting from the impact point of view of BG consumption as a functional food for potential prevention of metabolic and tumor diseases.
34444931	6	34	theme	metabolic	1061:1069	arg1	effects					1093:1099	antioxidant, metabolic, and hepatoprotective effects	1048:1099	antioxidant, metabolic, and hepatoprotective effects	1048:1099	Characterization of the BGE action in cancer cells revealed that antioxidant, metabolic, and hepatoprotective effects occur upon treatment as well as induction of maturation of acute myeloid leukemia cells.
34444931	1	35	theme	many	224:227	arg1	diseases					229:236	many diseases	224:236	many diseases	224:236	Over the centuries, humans have traditionally used garlic (Allium sativum L.) as a food ingredient (spice) and remedy for many diseases.
34444931	0	36	theme	Extract	93:99	arg1	Effects					43:49	In Vitro Beneficial Effects	23:49	In Vitro Beneficial Effects of Black Garlic (Allium sativum L.) Polar Extract	23:99	Metabolite Profile and In Vitro Beneficial Effects of Black Garlic (Allium sativum L.) Polar Extract.
34444931	0	36	theme	Extract	93:99	arg1	Profile					11:17	Metabolite Profile	0:17	Metabolite Profile	0:17	Metabolite Profile and In Vitro Beneficial Effects of Black Garlic (Allium sativum L.) Polar Extract.
34444931	4	37	theme	BG	641:642	arg1	extract					630:636	methanol extract	621:636	methanol extract of BG (BGE)	621:648	In this study, the metabolite profile of methanol extract of BG (BGE) was determined by high-performance liquid chromatography coupled to tandem mass spectrometry in high-resolution mode.
34444931	3	38	theme	heat-ageing	373:383	arg1	bulbs					398:402	heat-ageing white garlic bulbs	373:402	heat-ageing white garlic bulbs	373:402	More recently, black garlic (BG), made by heat-ageing white garlic bulbs, has increased its popularity in cuisine and traditional medicine around the world, but there is still limited information on its composition and potential beneficial effects.
34444931	0	39	theme	Beneficial	32:41	arg1	Effects					43:49	In Vitro Beneficial Effects	23:49	In Vitro Beneficial Effects of Black Garlic (Allium sativum L.) Polar Extract	23:99	Metabolite Profile and In Vitro Beneficial Effects of Black Garlic (Allium sativum L.) Polar Extract.
34444931	4	40	theme	metabolite	599:608	arg1	profile					610:616	the metabolite profile	595:616	the metabolite profile of methanol extract of BG (BGE)	595:648	In this study, the metabolite profile of methanol extract of BG (BGE) was determined by high-performance liquid chromatography coupled to tandem mass spectrometry in high-resolution mode.
34444931	3	41	theme	potential	550:558	arg1	effects					571:577	potential beneficial effects	550:577	potential beneficial effects	550:577	More recently, black garlic (BG), made by heat-ageing white garlic bulbs, has increased its popularity in cuisine and traditional medicine around the world, but there is still limited information on its composition and potential beneficial effects.
34444931	3	42	theme	black	346:350	arg1	BG					360:361	BG	360:361	BG	360:361	More recently, black garlic (BG), made by heat-ageing white garlic bulbs, has increased its popularity in cuisine and traditional medicine around the world, but there is still limited information on its composition and potential beneficial effects.
34444931	3	42	theme	black	346:350	arg1	garlic					352:357	black garlic	346:357	black garlic (BG)	346:362	More recently, black garlic (BG), made by heat-ageing white garlic bulbs, has increased its popularity in cuisine and traditional medicine around the world, but there is still limited information on its composition and potential beneficial effects.
34444931	7	43	from	point	1236:1240	arg1	interesting					1208:1218	interesting	1208:1218	interesting	1208:1218	These results are interesting from the impact point of view of BG consumption as a functional food for potential prevention of metabolic and tumor diseases.
34444931	7	44	theme	view	1245:1248	arg1	point					1236:1240	the impact point	1225:1240	the impact point of view of BG consumption	1225:1266	These results are interesting from the impact point of view of BG consumption as a functional food for potential prevention of metabolic and tumor diseases.
34444931	6	45	theme	maturation	1146:1155	arg1	treatment					1112:1120	treatment	1112:1120	treatment	1112:1120	Characterization of the BGE action in cancer cells revealed that antioxidant, metabolic, and hepatoprotective effects occur upon treatment as well as induction of maturation of acute myeloid leukemia cells.
34444931	6	45	theme	maturation	1146:1155	arg1	induction					1133:1141	induction	1133:1141	induction of maturation of acute myeloid leukemia cells	1133:1187	Characterization of the BGE action in cancer cells revealed that antioxidant, metabolic, and hepatoprotective effects occur upon treatment as well as induction of maturation of acute myeloid leukemia cells.
34444931	7	46	theme	BG	1253:1254	arg1	consumption					1256:1266	BG consumption	1253:1266	BG consumption	1253:1266	These results are interesting from the impact point of view of BG consumption as a functional food for potential prevention of metabolic and tumor diseases.
34444931	4	47	theme	mass	725:728	arg1	spectrometry					730:741	tandem mass spectrometry	718:741	tandem mass spectrometry in high-resolution mode	718:765	In this study, the metabolite profile of methanol extract of BG (BGE) was determined by high-performance liquid chromatography coupled to tandem mass spectrometry in high-resolution mode.
34444931	6	48	from	cells	1028:1032	arg1	Characterization					983:998	Characterization	983:998	Characterization of the BGE action in cancer cells	983:1032	Characterization of the BGE action in cancer cells revealed that antioxidant, metabolic, and hepatoprotective effects occur upon treatment as well as induction of maturation of acute myeloid leukemia cells.
34444931	6	49	theme	antioxidant	1048:1058	arg1	effects					1093:1099	antioxidant, metabolic, and hepatoprotective effects	1048:1099	antioxidant, metabolic, and hepatoprotective effects	1048:1099	Characterization of the BGE action in cancer cells revealed that antioxidant, metabolic, and hepatoprotective effects occur upon treatment as well as induction of maturation of acute myeloid leukemia cells.
34444931	5	50	theme	typical	940:946	arg1	derivatives					835:845	sulfur derivatives	828:845	sulfur derivatives	828:845	Results allowed to establish that BGE major components were sulfur derivatives, saccharides, peptides, organic acids, a phenylpropanoid derivative, saponins, and compounds typical of glycerophospholipid metabolism.
34444931	4	51	theme	tandem	718:723	arg1	spectrometry					730:741	tandem mass spectrometry	718:741	tandem mass spectrometry in high-resolution mode	718:765	In this study, the metabolite profile of methanol extract of BG (BGE) was determined by high-performance liquid chromatography coupled to tandem mass spectrometry in high-resolution mode.
34444931	2	52	theme	extensive	261:269	arg1	studies					271:277	many extensive studies	256:277	many extensive studies	256:277	To confirm this, many extensive studies recognized the therapeutic effects of garlic bulbs.
34444931	4	53	theme	liquid	685:690	arg1	chromatography					692:705	high-performance liquid chromatography	668:705	high-performance liquid chromatography coupled to tandem mass spectrometry in high-resolution mode	668:765	In this study, the metabolite profile of methanol extract of BG (BGE) was determined by high-performance liquid chromatography coupled to tandem mass spectrometry in high-resolution mode.
34444931	4	54	theme	high-performance	668:683	arg1	chromatography					692:705	high-performance liquid chromatography	668:705	high-performance liquid chromatography coupled to tandem mass spectrometry in high-resolution mode	668:765	In this study, the metabolite profile of methanol extract of BG (BGE) was determined by high-performance liquid chromatography coupled to tandem mass spectrometry in high-resolution mode.
34444931	0	55	dep	In	23:24	arg1	Vitro					26:30	Vitro	26:30	Vitro	26:30	Metabolite Profile and In Vitro Beneficial Effects of Black Garlic (Allium sativum L.) Polar Extract.
34444931	7	56	theme	potential	1293:1301	arg1	prevention					1303:1312	potential prevention	1293:1312	potential prevention of metabolic and tumor diseases	1293:1344	These results are interesting from the impact point of view of BG consumption as a functional food for potential prevention of metabolic and tumor diseases.
34444931	2	57	theme	many	256:259	arg1	studies					271:277	many extensive studies	256:277	many extensive studies	256:277	To confirm this, many extensive studies recognized the therapeutic effects of garlic bulbs.
34444931	7	58	theme	diseases	1337:1344	arg1	prevention					1303:1312	potential prevention	1293:1312	potential prevention of metabolic and tumor diseases	1293:1344	These results are interesting from the impact point of view of BG consumption as a functional food for potential prevention of metabolic and tumor diseases.
34444931	3	59	theme	beneficial	560:569	arg1	effects					571:577	potential beneficial effects	550:577	potential beneficial effects	550:577	More recently, black garlic (BG), made by heat-ageing white garlic bulbs, has increased its popularity in cuisine and traditional medicine around the world, but there is still limited information on its composition and potential beneficial effects.
34444931	4	60	theme	methanol	621:628	arg1	extract					630:636	methanol extract	621:636	methanol extract of BG (BGE)	621:648	In this study, the metabolite profile of methanol extract of BG (BGE) was determined by high-performance liquid chromatography coupled to tandem mass spectrometry in high-resolution mode.
34444931	6	61	from	action	1011:1016	arg1	cells					1028:1032	cancer cells	1021:1032	cancer cells	1021:1032	Characterization of the BGE action in cancer cells revealed that antioxidant, metabolic, and hepatoprotective effects occur upon treatment as well as induction of maturation of acute myeloid leukemia cells.
34444931	3	62	theme	garlic	391:396	arg1	bulbs					398:402	heat-ageing white garlic bulbs	373:402	heat-ageing white garlic bulbs	373:402	More recently, black garlic (BG), made by heat-ageing white garlic bulbs, has increased its popularity in cuisine and traditional medicine around the world, but there is still limited information on its composition and potential beneficial effects.
34444931	3	63	theme	white	385:389	arg1	bulbs					398:402	heat-ageing white garlic bulbs	373:402	heat-ageing white garlic bulbs	373:402	More recently, black garlic (BG), made by heat-ageing white garlic bulbs, has increased its popularity in cuisine and traditional medicine around the world, but there is still limited information on its composition and potential beneficial effects.
34444931	0	64	theme	Garlic	60:65	arg1	Extract					93:99	Black Garlic (Allium sativum L.) Polar Extract	54:99	Black Garlic (Allium sativum L.) Polar Extract	54:99	Metabolite Profile and In Vitro Beneficial Effects of Black Garlic (Allium sativum L.) Polar Extract.
34444931	5	65	theme	metabolism	971:980	arg1	typical					940:946	typical	940:946	typical	940:946	Results allowed to establish that BGE major components were sulfur derivatives, saccharides, peptides, organic acids, a phenylpropanoid derivative, saponins, and compounds typical of glycerophospholipid metabolism.
34444931	1	66	used	used	148:151	arg2	humans					122:127	humans	122:127	humans	122:127	Over the centuries, humans have traditionally used garlic (Allium sativum L.) as a food ingredient (spice) and remedy for many diseases.
34444931	6	67	theme	cancer	1021:1026	arg1	cells					1028:1032	cancer cells	1021:1032	cancer cells	1021:1032	Characterization of the BGE action in cancer cells revealed that antioxidant, metabolic, and hepatoprotective effects occur upon treatment as well as induction of maturation of acute myeloid leukemia cells.
34444931	1	68	theme	food	185:188	arg1	garlic					153:158	garlic	153:158	garlic (Allium sativum L.)	153:178	Over the centuries, humans have traditionally used garlic (Allium sativum L.) as a food ingredient (spice) and remedy for many diseases.
34444931	1	68	theme	food	185:188	arg1	spice					202:206	spice	202:206	spice	202:206	Over the centuries, humans have traditionally used garlic (Allium sativum L.) as a food ingredient (spice) and remedy for many diseases.
34444931	1	68	theme	food	185:188	arg1	ingredient					190:199	a food ingredient	183:199	a food ingredient (spice)	183:207	Over the centuries, humans have traditionally used garlic (Allium sativum L.) as a food ingredient (spice) and remedy for many diseases.
34444931	0	69	theme	Black	54:58	arg1	Allium					68:73	Allium	68:73	Allium	68:73	Metabolite Profile and In Vitro Beneficial Effects of Black Garlic (Allium sativum L.) Polar Extract.
34444931	0	69	theme	Black	54:58	arg1	Garlic					60:65	Black Garlic	54:65	Black Garlic (Allium sativum L.) Polar Extract	54:99	Metabolite Profile and In Vitro Beneficial Effects of Black Garlic (Allium sativum L.) Polar Extract.
34444931	3	70	theme	limited	507:513	arg1	information					515:525	limited information	507:525	limited information on its composition and potential beneficial effects	507:577	More recently, black garlic (BG), made by heat-ageing white garlic bulbs, has increased its popularity in cuisine and traditional medicine around the world, but there is still limited information on its composition and potential beneficial effects.
34444931	2	71	theme	therapeutic	294:304	arg1	effects					306:312	the therapeutic effects	290:312	the therapeutic effects of garlic bulbs	290:328	To confirm this, many extensive studies recognized the therapeutic effects of garlic bulbs.
31954790	0	0	theme	green	88:92	arg1	removal					67:73	efficient removal	57:73	efficient removal of malachite green dye	57:96	Graphite modified sodium alginate hydrogel composite for efficient removal of malachite green dye.
31954790	6	1	theme	Langmuir	1004:1011	arg1	model					1022:1026	the Langmuir isotherm model	1000:1026	the Langmuir isotherm model	1000:1026	Moreover, the adsorption process could be well described by the Langmuir isotherm model and pseudo-second-order kinetic model.
31954790	4	2	theme	photoelectron	764:776	arg1	spectroscopy					778:789	x-ray photoelectron spectroscopy	758:789	x-ray photoelectron spectroscopy	758:789	The NaA-cl-AAc/GP hydrogel composite was characterized by infrared spectroscopy, Raman spectroscopy, thermo-gravimetric analysis, scanning electron microscopy, x-ray photoelectron spectroscopy and x-ray diffraction.
31954790	0	3	theme	malachite	78:86	arg1	green					88:92	malachite green dye	78:96	malachite green dye	78:96	Graphite modified sodium alginate hydrogel composite for efficient removal of malachite green dye.
31954790	3	4	theme	dye	536:538	arg1	removal					504:510	the removal	500:510	the removal of malachite green (MG) dye from aqueous solution	500:560	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite was utilized in the removal of malachite green (MG) dye from aqueous solution using batch adsorption experiments.
31954790	4	5	theme	hydrogel	616:623	arg1	composite					625:633	The NaA-cl-AAc/GP hydrogel composite	598:633	The NaA-cl-AAc/GP hydrogel composite	598:633	The NaA-cl-AAc/GP hydrogel composite was characterized by infrared spectroscopy, Raman spectroscopy, thermo-gravimetric analysis, scanning electron microscopy, x-ray photoelectron spectroscopy and x-ray diffraction.
31954790	8	6	theme	better	1310:1315	arg1	rate					1326:1329	its better swelling rate	1306:1329	its better swelling rate	1306:1329	Therefore, the NaA-cl-AAc/GP hydrogel composite is a potentially favourable material towards dye pollution remediation owing to its better swelling rate, environment friendliness, high adsorption potential and regeneration capability.
31954790	7	7	theme	hydrogel	1071:1078	arg1	composite					1080:1088	The hydrogel composite	1067:1088	The hydrogel composite	1067:1088	The hydrogel composite also showed 91% adsorption after three consecutive cycles of dye adsorption-desorption.
31954790	3	8	theme	batch	568:572	arg1	experiments					585:595	batch adsorption experiments	568:595	batch adsorption experiments	568:595	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite was utilized in the removal of malachite green (MG) dye from aqueous solution using batch adsorption experiments.
31954790	3	9	theme	synthesized	386:396	arg1	composite					474:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite	382:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite	382:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite was utilized in the removal of malachite green (MG) dye from aqueous solution using batch adsorption experiments.
31954790	3	10	theme	adsorption	574:583	arg1	experiments					585:595	batch adsorption experiments	568:595	batch adsorption experiments	568:595	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite was utilized in the removal of malachite green (MG) dye from aqueous solution using batch adsorption experiments.
31954790	3	11	theme	green	525:529	arg1	dye					536:538	malachite green (MG) dye	515:538	malachite green (MG) dye	515:538	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite was utilized in the removal of malachite green (MG) dye from aqueous solution using batch adsorption experiments.
31954790	5	12	theme	optimized	820:828	arg1	conditions					843:852	optimized experimental conditions	820:852	optimized experimental conditions	820:852	Under optimized experimental conditions, a maximum adsorption capacity of 628.93 mg g-1 was attained for malachite green dye.
31954790	2	13	theme	adsorption	359:368	arg1	capability					370:379	its adsorption capability	355:379	its adsorption capability	355:379	Sodium alginate was modified through graft polymerization of acrylic acid and subsequently loaded with graphite powder to enhance its adsorption capability.
31954790	4	14	theme	Raman	679:683	arg1	spectroscopy					685:696	Raman spectroscopy	679:696	Raman spectroscopy	679:696	The NaA-cl-AAc/GP hydrogel composite was characterized by infrared spectroscopy, Raman spectroscopy, thermo-gravimetric analysis, scanning electron microscopy, x-ray photoelectron spectroscopy and x-ray diffraction.
31954790	3	15	used	utilized	488:495	arg2	composite					474:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite	382:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite	382:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite was utilized in the removal of malachite green (MG) dye from aqueous solution using batch adsorption experiments.
31954790	4	16	theme	electron	737:744	arg1	microscopy					746:755	scanning electron microscopy	728:755	scanning electron microscopy	728:755	The NaA-cl-AAc/GP hydrogel composite was characterized by infrared spectroscopy, Raman spectroscopy, thermo-gravimetric analysis, scanning electron microscopy, x-ray photoelectron spectroscopy and x-ray diffraction.
31954790	5	17	theme	experimental	830:841	arg1	conditions					843:852	optimized experimental conditions	820:852	optimized experimental conditions	820:852	Under optimized experimental conditions, a maximum adsorption capacity of 628.93 mg g-1 was attained for malachite green dye.
31954790	5	18	theme	adsorption	865:874	arg1	capacity					876:883	a maximum adsorption capacity	855:883	a maximum adsorption capacity of 628.93 mg g-1	855:900	Under optimized experimental conditions, a maximum adsorption capacity of 628.93 mg g-1 was attained for malachite green dye.
31954790	2	19	theme	acrylic	286:292	arg1	acid					294:297	acrylic acid	286:297	acrylic acid	286:297	Sodium alginate was modified through graft polymerization of acrylic acid and subsequently loaded with graphite powder to enhance its adsorption capability.
31954790	8	20	theme	adsorption	1363:1372	arg1	potential					1374:1382	high adsorption potential	1358:1382	high adsorption potential	1358:1382	Therefore, the NaA-cl-AAc/GP hydrogel composite is a potentially favourable material towards dye pollution remediation owing to its better swelling rate, environment friendliness, high adsorption potential and regeneration capability.
31954790	3	21	theme	cross-linked	414:425	arg1	composite					474:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite	382:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite	382:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite was utilized in the removal of malachite green (MG) dye from aqueous solution using batch adsorption experiments.
31954790	5	22	theme	maximum	857:863	arg1	capacity					876:883	a maximum adsorption capacity	855:883	a maximum adsorption capacity of 628.93 mg g-1	855:900	Under optimized experimental conditions, a maximum adsorption capacity of 628.93 mg g-1 was attained for malachite green dye.
31954790	3	23	theme	hydrogel	465:472	arg1	composite					474:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite	382:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite	382:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite was utilized in the removal of malachite green (MG) dye from aqueous solution using batch adsorption experiments.
31954790	3	24	theme	acrylic	427:433	arg1	composite					474:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite	382:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite	382:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite was utilized in the removal of malachite green (MG) dye from aqueous solution using batch adsorption experiments.
31954790	8	25	theme	hydrogel	1207:1214	arg1	material					1254:1261	a potentially favourable material	1229:1261	a potentially favourable material towards dye pollution remediation owing to its better swelling rate, environment friendliness, high adsorption potential and regeneration capability	1229:1410	Therefore, the NaA-cl-AAc/GP hydrogel composite is a potentially favourable material towards dye pollution remediation owing to its better swelling rate, environment friendliness, high adsorption potential and regeneration capability.
31954790	8	25	theme	hydrogel	1207:1214	arg1	composite					1216:1224	the NaA-cl-AAc/GP hydrogel composite	1189:1224	the NaA-cl-AAc/GP hydrogel composite	1189:1224	Therefore, the NaA-cl-AAc/GP hydrogel composite is a potentially favourable material towards dye pollution remediation owing to its better swelling rate, environment friendliness, high adsorption potential and regeneration capability.
31954790	4	26	theme	x-ray	795:799	arg1	diffraction					801:811	x-ray diffraction	795:811	x-ray diffraction	795:811	The NaA-cl-AAc/GP hydrogel composite was characterized by infrared spectroscopy, Raman spectroscopy, thermo-gravimetric analysis, scanning electron microscopy, x-ray photoelectron spectroscopy and x-ray diffraction.
31954790	2	27	theme	graphite	328:335	arg1	powder					337:342	graphite powder	328:342	graphite powder	328:342	Sodium alginate was modified through graft polymerization of acrylic acid and subsequently loaded with graphite powder to enhance its adsorption capability.
31954790	8	28	theme	pollution	1275:1283	arg1	remediation					1285:1295	dye pollution remediation	1271:1295	dye pollution remediation	1271:1295	Therefore, the NaA-cl-AAc/GP hydrogel composite is a potentially favourable material towards dye pollution remediation owing to its better swelling rate, environment friendliness, high adsorption potential and regeneration capability.
31954790	4	29	theme	scanning	728:735	arg1	microscopy					746:755	scanning electron microscopy	728:755	scanning electron microscopy	728:755	The NaA-cl-AAc/GP hydrogel composite was characterized by infrared spectroscopy, Raman spectroscopy, thermo-gravimetric analysis, scanning electron microscopy, x-ray photoelectron spectroscopy and x-ray diffraction.
31954790	0	30	theme	alginate	25:32	arg1	composite					43:51	sodium alginate hydrogel composite	18:51	sodium alginate hydrogel composite for efficient removal of malachite green dye	18:96	Graphite modified sodium alginate hydrogel composite for efficient removal of malachite green dye.
31954790	2	31	theme	graft	262:266	arg1	polymerization					268:281	graft polymerization	262:281	graft polymerization of acrylic acid	262:297	Sodium alginate was modified through graft polymerization of acrylic acid and subsequently loaded with graphite powder to enhance its adsorption capability.
31954790	8	32	theme	NaA-cl-AAc/GP	1193:1205	arg1	material					1254:1261	a potentially favourable material	1229:1261	a potentially favourable material towards dye pollution remediation owing to its better swelling rate, environment friendliness, high adsorption potential and regeneration capability	1229:1410	Therefore, the NaA-cl-AAc/GP hydrogel composite is a potentially favourable material towards dye pollution remediation owing to its better swelling rate, environment friendliness, high adsorption potential and regeneration capability.
31954790	8	32	theme	NaA-cl-AAc/GP	1193:1205	arg1	composite					1216:1224	the NaA-cl-AAc/GP hydrogel composite	1189:1224	the NaA-cl-AAc/GP hydrogel composite	1189:1224	Therefore, the NaA-cl-AAc/GP hydrogel composite is a potentially favourable material towards dye pollution remediation owing to its better swelling rate, environment friendliness, high adsorption potential and regeneration capability.
31954790	0	33	theme	sodium	18:23	arg1	composite					43:51	sodium alginate hydrogel composite	18:51	sodium alginate hydrogel composite for efficient removal of malachite green dye	18:96	Graphite modified sodium alginate hydrogel composite for efficient removal of malachite green dye.
31954790	4	34	theme	NaA-cl-AAc/GP	602:614	arg1	composite					625:633	The NaA-cl-AAc/GP hydrogel composite	598:633	The NaA-cl-AAc/GP hydrogel composite	598:633	The NaA-cl-AAc/GP hydrogel composite was characterized by infrared spectroscopy, Raman spectroscopy, thermo-gravimetric analysis, scanning electron microscopy, x-ray photoelectron spectroscopy and x-ray diffraction.
31954790	2	35	theme	acid	294:297	arg1	polymerization					268:281	graft polymerization	262:281	graft polymerization of acrylic acid	262:297	Sodium alginate was modified through graft polymerization of acrylic acid and subsequently loaded with graphite powder to enhance its adsorption capability.
31954790	4	36	theme	x-ray	758:762	arg1	spectroscopy					778:789	x-ray photoelectron spectroscopy	758:789	x-ray photoelectron spectroscopy	758:789	The NaA-cl-AAc/GP hydrogel composite was characterized by infrared spectroscopy, Raman spectroscopy, thermo-gravimetric analysis, scanning electron microscopy, x-ray photoelectron spectroscopy and x-ray diffraction.
31954790	1	37	theme	organic	206:212	arg1	pollutant					214:222	organic pollutant	206:222	organic pollutant	206:222	Herein, porous sodium alginate/graphite based hybrid hydrogel was fabricated as an effective adsorbent for organic pollutant.
31954790	3	38	theme	NaA-cl-AAc/GP	450:462	arg1	composite					474:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite	382:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite	382:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite was utilized in the removal of malachite green (MG) dye from aqueous solution using batch adsorption experiments.
31954790	7	39	theme	adsorption-desorption	1155:1175	arg1	cycles					1141:1146	three consecutive cycles	1123:1146	three consecutive cycles of dye adsorption-desorption	1123:1175	The hydrogel composite also showed 91% adsorption after three consecutive cycles of dye adsorption-desorption.
31954790	6	40	theme	adsorption	954:963	arg1	process					965:971	the adsorption process	950:971	the adsorption process	950:971	Moreover, the adsorption process could be well described by the Langmuir isotherm model and pseudo-second-order kinetic model.
31954790	4	41	theme	thermo-gravimetric	699:716	arg1	analysis					718:725	thermo-gravimetric analysis	699:725	thermo-gravimetric analysis	699:725	The NaA-cl-AAc/GP hydrogel composite was characterized by infrared spectroscopy, Raman spectroscopy, thermo-gravimetric analysis, scanning electron microscopy, x-ray photoelectron spectroscopy and x-ray diffraction.
31954790	0	42	theme	hydrogel	34:41	arg1	composite					43:51	sodium alginate hydrogel composite	18:51	sodium alginate hydrogel composite for efficient removal of malachite green dye	18:96	Graphite modified sodium alginate hydrogel composite for efficient removal of malachite green dye.
31954790	6	43	theme	kinetic	1052:1058	arg1	model					1060:1064	pseudo-second-order kinetic model	1032:1064	pseudo-second-order kinetic model	1032:1064	Moreover, the adsorption process could be well described by the Langmuir isotherm model and pseudo-second-order kinetic model.
31954790	8	44	theme	environment	1332:1342	arg1	friendliness					1344:1355	environment friendliness	1332:1355	environment friendliness	1332:1355	Therefore, the NaA-cl-AAc/GP hydrogel composite is a potentially favourable material towards dye pollution remediation owing to its better swelling rate, environment friendliness, high adsorption potential and regeneration capability.
31954790	8	45	theme	dye	1271:1273	arg1	remediation					1285:1295	dye pollution remediation	1271:1295	dye pollution remediation	1271:1295	Therefore, the NaA-cl-AAc/GP hydrogel composite is a potentially favourable material towards dye pollution remediation owing to its better swelling rate, environment friendliness, high adsorption potential and regeneration capability.
31954790	3	46	theme	sodium	398:403	arg1	composite					474:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite	382:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite	382:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite was utilized in the removal of malachite green (MG) dye from aqueous solution using batch adsorption experiments.
31954790	8	47	theme	swelling	1317:1324	arg1	rate					1326:1329	its better swelling rate	1306:1329	its better swelling rate	1306:1329	Therefore, the NaA-cl-AAc/GP hydrogel composite is a potentially favourable material towards dye pollution remediation owing to its better swelling rate, environment friendliness, high adsorption potential and regeneration capability.
31954790	0	48	theme	efficient	57:65	arg1	removal					67:73	efficient removal	57:73	efficient removal of malachite green dye	57:96	Graphite modified sodium alginate hydrogel composite for efficient removal of malachite green dye.
31954790	6	49	theme	pseudo-second-order	1032:1050	arg1	model					1060:1064	pseudo-second-order kinetic model	1032:1064	pseudo-second-order kinetic model	1032:1064	Moreover, the adsorption process could be well described by the Langmuir isotherm model and pseudo-second-order kinetic model.
31954790	8	50	theme	favourable	1243:1252	arg1	material					1254:1261	a potentially favourable material	1229:1261	a potentially favourable material towards dye pollution remediation owing to its better swelling rate, environment friendliness, high adsorption potential and regeneration capability	1229:1410	Therefore, the NaA-cl-AAc/GP hydrogel composite is a potentially favourable material towards dye pollution remediation owing to its better swelling rate, environment friendliness, high adsorption potential and regeneration capability.
31954790	8	50	theme	favourable	1243:1252	arg1	composite					1216:1224	the NaA-cl-AAc/GP hydrogel composite	1189:1224	the NaA-cl-AAc/GP hydrogel composite	1189:1224	Therefore, the NaA-cl-AAc/GP hydrogel composite is a potentially favourable material towards dye pollution remediation owing to its better swelling rate, environment friendliness, high adsorption potential and regeneration capability.
31954790	1	51	theme	porous	107:112	arg1	effective					182:190	effective	182:190	effective	182:190	Herein, porous sodium alginate/graphite based hybrid hydrogel was fabricated as an effective adsorbent for organic pollutant.
31954790	1	51	theme	porous	107:112	arg1	hydrogel					152:159	porous sodium alginate/graphite based hybrid hydrogel	107:159	porous sodium alginate/graphite based hybrid hydrogel	107:159	Herein, porous sodium alginate/graphite based hybrid hydrogel was fabricated as an effective adsorbent for organic pollutant.
31954790	5	52	theme	628.93 mg g-1	888:900	arg1	capacity					876:883	a maximum adsorption capacity	855:883	a maximum adsorption capacity of 628.93 mg g-1	855:900	Under optimized experimental conditions, a maximum adsorption capacity of 628.93 mg g-1 was attained for malachite green dye.
31954790	7	53	theme	dye	1151:1153	arg1	adsorption-desorption					1155:1175	dye adsorption-desorption	1151:1175	dye adsorption-desorption	1151:1175	The hydrogel composite also showed 91% adsorption after three consecutive cycles of dye adsorption-desorption.
31954790	2	54	theme	Sodium	225:230	arg1	alginate					232:239	Sodium alginate	225:239	Sodium alginate	225:239	Sodium alginate was modified through graft polymerization of acrylic acid and subsequently loaded with graphite powder to enhance its adsorption capability.
31954790	7	55	theme	%	1104:1104	arg1	adsorption					1106:1115	91% adsorption	1102:1115	91% adsorption	1102:1115	The hydrogel composite also showed 91% adsorption after three consecutive cycles of dye adsorption-desorption.
31954790	1	56	theme	sodium	114:119	arg1	effective					182:190	effective	182:190	effective	182:190	Herein, porous sodium alginate/graphite based hybrid hydrogel was fabricated as an effective adsorbent for organic pollutant.
31954790	1	56	theme	sodium	114:119	arg1	hydrogel					152:159	porous sodium alginate/graphite based hybrid hydrogel	107:159	porous sodium alginate/graphite based hybrid hydrogel	107:159	Herein, porous sodium alginate/graphite based hybrid hydrogel was fabricated as an effective adsorbent for organic pollutant.
31954790	7	57	theme	consecutive	1129:1139	arg1	cycles					1141:1146	three consecutive cycles	1123:1146	three consecutive cycles of dye adsorption-desorption	1123:1175	The hydrogel composite also showed 91% adsorption after three consecutive cycles of dye adsorption-desorption.
31954790	8	58	theme	regeneration	1388:1399	arg1	capability					1401:1410	regeneration capability	1388:1410	regeneration capability	1388:1410	Therefore, the NaA-cl-AAc/GP hydrogel composite is a potentially favourable material towards dye pollution remediation owing to its better swelling rate, environment friendliness, high adsorption potential and regeneration capability.
31954790	1	59	theme	alginate/graphite	121:137	arg1	effective					182:190	effective	182:190	effective	182:190	Herein, porous sodium alginate/graphite based hybrid hydrogel was fabricated as an effective adsorbent for organic pollutant.
31954790	1	59	theme	alginate/graphite	121:137	arg1	hydrogel					152:159	porous sodium alginate/graphite based hybrid hydrogel	107:159	porous sodium alginate/graphite based hybrid hydrogel	107:159	Herein, porous sodium alginate/graphite based hybrid hydrogel was fabricated as an effective adsorbent for organic pollutant.
31954790	4	60	theme	infrared	656:663	arg1	spectroscopy					665:676	infrared spectroscopy	656:676	infrared spectroscopy	656:676	The NaA-cl-AAc/GP hydrogel composite was characterized by infrared spectroscopy, Raman spectroscopy, thermo-gravimetric analysis, scanning electron microscopy, x-ray photoelectron spectroscopy and x-ray diffraction.
31954790	5	61	theme	malachite	919:927	arg1	green					929:933	malachite green dye	919:937	malachite green dye	919:937	Under optimized experimental conditions, a maximum adsorption capacity of 628.93 mg g-1 was attained for malachite green dye.
31954790	1	62	theme	based	139:143	arg1	effective					182:190	effective	182:190	effective	182:190	Herein, porous sodium alginate/graphite based hybrid hydrogel was fabricated as an effective adsorbent for organic pollutant.
31954790	1	62	theme	based	139:143	arg1	hydrogel					152:159	porous sodium alginate/graphite based hybrid hydrogel	107:159	porous sodium alginate/graphite based hybrid hydrogel	107:159	Herein, porous sodium alginate/graphite based hybrid hydrogel was fabricated as an effective adsorbent for organic pollutant.
31954790	8	63	theme	high	1358:1361	arg1	potential					1374:1382	high adsorption potential	1358:1382	high adsorption potential	1358:1382	Therefore, the NaA-cl-AAc/GP hydrogel composite is a potentially favourable material towards dye pollution remediation owing to its better swelling rate, environment friendliness, high adsorption potential and regeneration capability.
31954790	3	64	theme	malachite	515:523	arg1	MG					532:533	MG	532:533	MG	532:533	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite was utilized in the removal of malachite green (MG) dye from aqueous solution using batch adsorption experiments.
31954790	3	64	theme	malachite	515:523	arg1	green					525:529	malachite green	515:529	malachite green (MG) dye	515:538	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite was utilized in the removal of malachite green (MG) dye from aqueous solution using batch adsorption experiments.
31954790	3	65	link	cross-linked	414:425	arg1	composite					474:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite	382:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite	382:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite was utilized in the removal of malachite green (MG) dye from aqueous solution using batch adsorption experiments.
31954790	7	66	theme	91	1102:1103	arg1	%					1104:1104	%	1104:1104	%	1104:1104	The hydrogel composite also showed 91% adsorption after three consecutive cycles of dye adsorption-desorption.
31954790	1	67	theme	hybrid	145:150	arg1	effective					182:190	effective	182:190	effective	182:190	Herein, porous sodium alginate/graphite based hybrid hydrogel was fabricated as an effective adsorbent for organic pollutant.
31954790	1	67	theme	hybrid	145:150	arg1	hydrogel					152:159	porous sodium alginate/graphite based hybrid hydrogel	107:159	porous sodium alginate/graphite based hybrid hydrogel	107:159	Herein, porous sodium alginate/graphite based hybrid hydrogel was fabricated as an effective adsorbent for organic pollutant.
31954790	3	68	theme	acid/graphite	435:447	arg1	composite					474:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite	382:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite	382:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite was utilized in the removal of malachite green (MG) dye from aqueous solution using batch adsorption experiments.
31954790	6	69	theme	isotherm	1013:1020	arg1	model					1022:1026	the Langmuir isotherm model	1000:1026	the Langmuir isotherm model	1000:1026	Moreover, the adsorption process could be well described by the Langmuir isotherm model and pseudo-second-order kinetic model.
31954790	3	70	theme	alginate	405:412	arg1	composite					474:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite	382:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite	382:482	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite was utilized in the removal of malachite green (MG) dye from aqueous solution using batch adsorption experiments.
31954790	3	71	theme	aqueous	545:551	arg1	solution					553:560	aqueous solution	545:560	aqueous solution	545:560	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite was utilized in the removal of malachite green (MG) dye from aqueous solution using batch adsorption experiments.
31954790	3	72	from	solution	553:560	arg1	removal					504:510	the removal	500:510	the removal of malachite green (MG) dye from aqueous solution	500:560	The synthesized sodium alginate cross-linked acrylic acid/graphite (NaA-cl-AAc/GP) hydrogel composite was utilized in the removal of malachite green (MG) dye from aqueous solution using batch adsorption experiments.
33572006	5	0	with	line	1237:1240	arg1	attributes					1276:1285	some experimental structural attributes	1247:1285	some experimental structural attributes	1247:1285	The properties simulated in this work are meant to provide a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties and are in line with some experimental structural attributes.
33572006	3	1	theme	β	642:642	arg1	ratio					629:633	the ratio	625:633	the ratio of the β 1-4 and β 1-3 linked residues	625:672	In mixed β 1-4/1-3 d-glucopyranosides, as those found in oats and barley, the ratio of the β 1-4 and β 1-3 linked residues is crucial in determining the structural and dynamical attributes.
33572006	3	1	theme	β	642:642	arg1	crucial					677:683	crucial	677:683	crucial	677:683	In mixed β 1-4/1-3 d-glucopyranosides, as those found in oats and barley, the ratio of the β 1-4 and β 1-3 linked residues is crucial in determining the structural and dynamical attributes.
33572006	1	2	theme	several	134:140	arg1	oligosaccharides					142:157	several oligosaccharides	134:157	several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages	134:313	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33572006	3	3	theme	β	652:652	arg1	residues					665:672	the β 1-4 and β 1-3 linked residues	638:672	residues	665:672	In mixed β 1-4/1-3 d-glucopyranosides, as those found in oats and barley, the ratio of the β 1-4 and β 1-3 linked residues is crucial in determining the structural and dynamical attributes.
33572006	4	4	theme	oxygen	849:854	arg1	atoms					856:860	glycosidic oxygen atoms	838:860	glycosidic oxygen atoms	838:860	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms and subsequent K-means clustering of the dynamical space spanned by PC1 to PC2 point to the dynamical and structural disparity in the various types of oligosaccharides studied.
33572006	1	5	theme	glycosidic	295:304	arg1	linkages					306:313	glycosidic linkages	295:313	glycosidic linkages	295:313	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33572006	4	6	theme	internal	786:793	arg1	coordinates					795:805	the internal coordinates	782:805	the internal coordinates of torsion angles subtended by glycosidic oxygen atoms	782:860	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms and subsequent K-means clustering of the dynamical space spanned by PC1 to PC2 point to the dynamical and structural disparity in the various types of oligosaccharides studied.
33572006	1	7	theme	oligosaccharides	142:157	arg1	study					125:129	a molecular dynamics simulation study	93:129	a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages	93:313	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33572006	5	8	theme	oligosaccharide	1193:1207	arg1	properties					1215:1224	the oligosaccharide chain properties	1189:1224	the oligosaccharide chain properties	1189:1224	The properties simulated in this work are meant to provide a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties and are in line with some experimental structural attributes.
33572006	1	9	theme	β-d	230:232	arg1	carbohydrate					206:217	carbohydrate	206:217	carbohydrate	206:217	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33572006	1	9	theme	β-d	230:232	arg1	units					248:252	the α- or β-d glucopyranose units	220:252	the α- or β-d glucopyranose units	220:252	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33572006	2	10	theme	anomeric	493:500	arg1	nature					502:507	anomeric nature	493:507	anomeric nature	493:507	Results indicate that the litheness of the oligosaccharide chain is noticeably controlled by the composition, anomeric nature and glycosidic linkage type of the units.
33572006	4	11	theme	torsion	810:816	arg1	angles					818:823	torsion angles	810:823	torsion angles subtended by glycosidic oxygen atoms	810:860	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms and subsequent K-means clustering of the dynamical space spanned by PC1 to PC2 point to the dynamical and structural disparity in the various types of oligosaccharides studied.
33572006	5	12	theme	chain	1209:1213	arg1	properties					1215:1224	the oligosaccharide chain properties	1189:1224	the oligosaccharide chain properties	1189:1224	The properties simulated in this work are meant to provide a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties and are in line with some experimental structural attributes.
33572006	1	13	theme	glucopyranose	234:246	arg1	carbohydrate					206:217	carbohydrate	206:217	carbohydrate	206:217	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33572006	1	13	theme	glucopyranose	234:246	arg1	units					248:252	the α- or β-d glucopyranose units	220:252	the α- or β-d glucopyranose units	220:252	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33572006	4	14	theme	K-means	877:883	arg1	clustering					885:894	subsequent K-means clustering	866:894	subsequent K-means clustering of the dynamical space spanned by PC1 to PC2	866:939	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms and subsequent K-means clustering of the dynamical space spanned by PC1 to PC2 point to the dynamical and structural disparity in the various types of oligosaccharides studied.
33572006	0	15	from	dynamics	51:58	arg1	water					83:87	water	83:87	water	83:87	Relation between glycosidic linkage, structure and dynamics of α- and β-glucans in water.
33572006	5	16	theme	anomericity	1174:1184	arg1	importance					1148:1157	the importance	1144:1157	the importance of linkage and anomericity on the oligosaccharide chain properties	1144:1224	The properties simulated in this work are meant to provide a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties and are in line with some experimental structural attributes.
33572006	4	17	from	disparity	979:987	arg1	types					1004:1008	the various types	992:1008	the various types of oligosaccharides studied	992:1036	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms and subsequent K-means clustering of the dynamical space spanned by PC1 to PC2 point to the dynamical and structural disparity in the various types of oligosaccharides studied.
33572006	5	18	theme	structural	1265:1274	arg1	attributes					1276:1285	some experimental structural attributes	1247:1285	some experimental structural attributes	1247:1285	The properties simulated in this work are meant to provide a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties and are in line with some experimental structural attributes.
33572006	5	19	theme	importance	1148:1157	arg1	understanding					1127:1139	a systematic yet comparative understanding	1098:1139	a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties	1098:1224	The properties simulated in this work are meant to provide a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties and are in line with some experimental structural attributes.
33572006	2	20	theme	composition	480:490	arg1	type					532:535	the composition, anomeric nature and glycosidic linkage type	476:535	the composition, anomeric nature and glycosidic linkage type of the units	476:548	Results indicate that the litheness of the oligosaccharide chain is noticeably controlled by the composition, anomeric nature and glycosidic linkage type of the units.
33572006	4	21	theme	structural	968:977	arg1	disparity					979:987	the dynamical and structural disparity	950:987	the dynamical and structural disparity in the various types of oligosaccharides studied	950:1036	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms and subsequent K-means clustering of the dynamical space spanned by PC1 to PC2 point to the dynamical and structural disparity in the various types of oligosaccharides studied.
33572006	3	22	from	d-glucopyranosides	570:587	arg1	ratio					629:633	the ratio	625:633	the ratio of the β 1-4 and β 1-3 linked residues	625:672	In mixed β 1-4/1-3 d-glucopyranosides, as those found in oats and barley, the ratio of the β 1-4 and β 1-3 linked residues is crucial in determining the structural and dynamical attributes.
33572006	3	22	from	d-glucopyranosides	570:587	arg1	crucial					677:683	crucial	677:683	crucial	677:683	In mixed β 1-4/1-3 d-glucopyranosides, as those found in oats and barley, the ratio of the β 1-4 and β 1-3 linked residues is crucial in determining the structural and dynamical attributes.
33572006	0	23	from	structure	37:45	arg1	water					83:87	water	83:87	water	83:87	Relation between glycosidic linkage, structure and dynamics of α- and β-glucans in water.
33572006	5	24	theme	simulated	1054:1062	arg1	properties					1043:1052	The properties	1039:1052	The properties simulated in this work	1039:1075	The properties simulated in this work are meant to provide a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties and are in line with some experimental structural attributes.
33572006	5	24	theme	simulated	1054:1062	arg1	meant					1081:1085	meant	1081:1085	meant	1081:1085	The properties simulated in this work are meant to provide a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties and are in line with some experimental structural attributes.
33572006	5	25	theme	comparative	1115:1125	arg1	understanding					1127:1139	a systematic yet comparative understanding	1098:1139	a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties	1098:1224	The properties simulated in this work are meant to provide a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties and are in line with some experimental structural attributes.
33572006	4	26	theme	space	913:917	arg1	PCA					771:773	PCA	771:773	PCA	771:773	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms and subsequent K-means clustering of the dynamical space spanned by PC1 to PC2 point to the dynamical and structural disparity in the various types of oligosaccharides studied.
33572006	4	26	theme	space	913:917	arg1	analysis					761:768	Principal component analysis	741:768	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms	741:860	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms and subsequent K-means clustering of the dynamical space spanned by PC1 to PC2 point to the dynamical and structural disparity in the various types of oligosaccharides studied.
33572006	4	26	theme	space	913:917	arg1	clustering					885:894	subsequent K-means clustering	866:894	subsequent K-means clustering of the dynamical space spanned by PC1 to PC2	866:939	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms and subsequent K-means clustering of the dynamical space spanned by PC1 to PC2 point to the dynamical and structural disparity in the various types of oligosaccharides studied.
33572006	5	27	from	understanding	1127:1139	arg1	properties					1215:1224	the oligosaccharide chain properties	1189:1224	the oligosaccharide chain properties	1189:1224	The properties simulated in this work are meant to provide a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties and are in line with some experimental structural attributes.
33572006	3	28	from	crucial	677:683	arg1	d-glucopyranosides					570:587	mixed β 1-4/1-3 d-glucopyranosides	554:587	mixed β 1-4/1-3 d-glucopyranosides	554:587	In mixed β 1-4/1-3 d-glucopyranosides, as those found in oats and barley, the ratio of the β 1-4 and β 1-3 linked residues is crucial in determining the structural and dynamical attributes.
33572006	5	29	from	importance	1148:1157	arg1	properties					1215:1224	the oligosaccharide chain properties	1189:1224	the oligosaccharide chain properties	1189:1224	The properties simulated in this work are meant to provide a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties and are in line with some experimental structural attributes.
33572006	5	30	from	work	1072:1075	arg1	simulated					1054:1062	simulated	1054:1062	simulated	1054:1062	The properties simulated in this work are meant to provide a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties and are in line with some experimental structural attributes.
33572006	5	31	theme	systematic	1100:1109	arg1	understanding					1127:1139	a systematic yet comparative understanding	1098:1139	a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties	1098:1224	The properties simulated in this work are meant to provide a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties and are in line with some experimental structural attributes.
33572006	2	32	theme	linkage	524:530	arg1	type					532:535	the composition, anomeric nature and glycosidic linkage type	476:535	the composition, anomeric nature and glycosidic linkage type of the units	476:548	Results indicate that the litheness of the oligosaccharide chain is noticeably controlled by the composition, anomeric nature and glycosidic linkage type of the units.
33572006	5	33	from	properties	1215:1224	arg1	understanding					1127:1139	a systematic yet comparative understanding	1098:1139	a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties	1098:1224	The properties simulated in this work are meant to provide a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties and are in line with some experimental structural attributes.
33572006	4	34	theme	component	751:759	arg1	PCA					771:773	PCA	771:773	PCA	771:773	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms and subsequent K-means clustering of the dynamical space spanned by PC1 to PC2 point to the dynamical and structural disparity in the various types of oligosaccharides studied.
33572006	4	34	theme	component	751:759	arg1	analysis					761:768	Principal component analysis	741:768	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms	741:860	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms and subsequent K-means clustering of the dynamical space spanned by PC1 to PC2 point to the dynamical and structural disparity in the various types of oligosaccharides studied.
33572006	1	35	theme	basic	182:186	arg1	block					197:201	the very basic building block	173:201	the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages	173:313	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33572006	0	36	theme	glycosidic	17:26	arg1	linkage					28:34	glycosidic linkage	17:34	glycosidic linkage	17:34	Relation between glycosidic linkage, structure and dynamics of α- and β-glucans in water.
33572006	3	37	theme	linked	658:663	arg1	residues					665:672	the β 1-4 and β 1-3 linked residues	638:672	residues	665:672	In mixed β 1-4/1-3 d-glucopyranosides, as those found in oats and barley, the ratio of the β 1-4 and β 1-3 linked residues is crucial in determining the structural and dynamical attributes.
33572006	2	38	theme	glycosidic	513:522	arg1	linkage					524:530	glycosidic linkage	513:530	glycosidic linkage	513:530	Results indicate that the litheness of the oligosaccharide chain is noticeably controlled by the composition, anomeric nature and glycosidic linkage type of the units.
33572006	0	39	from	β-glucans	70:78	arg1	water					83:87	water	83:87	water	83:87	Relation between glycosidic linkage, structure and dynamics of α- and β-glucans in water.
33572006	1	40	theme	building	188:195	arg1	block					197:201	the very basic building block	173:201	the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages	173:313	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33572006	2	41	theme	chain	442:446	arg1	litheness					409:417	the litheness	405:417	the litheness of the oligosaccharide chain	405:446	Results indicate that the litheness of the oligosaccharide chain is noticeably controlled by the composition, anomeric nature and glycosidic linkage type of the units.
33572006	3	42	theme	residues	665:672	arg1	ratio					629:633	the ratio	625:633	the ratio of the β 1-4 and β 1-3 linked residues	625:672	In mixed β 1-4/1-3 d-glucopyranosides, as those found in oats and barley, the ratio of the β 1-4 and β 1-3 linked residues is crucial in determining the structural and dynamical attributes.
33572006	3	42	theme	residues	665:672	arg1	crucial					677:683	crucial	677:683	crucial	677:683	In mixed β 1-4/1-3 d-glucopyranosides, as those found in oats and barley, the ratio of the β 1-4 and β 1-3 linked residues is crucial in determining the structural and dynamical attributes.
33572006	4	43	theme	subsequent	866:875	arg1	clustering					885:894	subsequent K-means clustering	866:894	subsequent K-means clustering of the dynamical space spanned by PC1 to PC2	866:939	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms and subsequent K-means clustering of the dynamical space spanned by PC1 to PC2 point to the dynamical and structural disparity in the various types of oligosaccharides studied.
33572006	3	44	theme	mixed	554:558	arg1	d-glucopyranosides					570:587	mixed β 1-4/1-3 d-glucopyranosides	554:587	mixed β 1-4/1-3 d-glucopyranosides	554:587	In mixed β 1-4/1-3 d-glucopyranosides, as those found in oats and barley, the ratio of the β 1-4 and β 1-3 linked residues is crucial in determining the structural and dynamical attributes.
33572006	5	45	theme	experimental	1252:1263	arg1	attributes					1276:1285	some experimental structural attributes	1247:1285	some experimental structural attributes	1247:1285	The properties simulated in this work are meant to provide a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties and are in line with some experimental structural attributes.
33572006	2	46	theme	oligosaccharide	426:440	arg1	chain					442:446	the oligosaccharide chain	422:446	the oligosaccharide chain	422:446	Results indicate that the litheness of the oligosaccharide chain is noticeably controlled by the composition, anomeric nature and glycosidic linkage type of the units.
33572006	2	47	theme	nature	502:507	arg1	type					532:535	the composition, anomeric nature and glycosidic linkage type	476:535	the composition, anomeric nature and glycosidic linkage type of the units	476:548	Results indicate that the litheness of the oligosaccharide chain is noticeably controlled by the composition, anomeric nature and glycosidic linkage type of the units.
33572006	1	48	theme	structural	346:355	arg1	properties					371:380	their structural and dynamical properties	340:380	their structural and dynamical properties	340:380	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33572006	3	49	theme	β	560:560	arg1	d-glucopyranosides					570:587	mixed β 1-4/1-3 d-glucopyranosides	554:587	mixed β 1-4/1-3 d-glucopyranosides	554:587	In mixed β 1-4/1-3 d-glucopyranosides, as those found in oats and barley, the ratio of the β 1-4 and β 1-3 linked residues is crucial in determining the structural and dynamical attributes.
33572006	3	50	located	found	599:603	arg2	those					593:597	those	593:597	those	593:597	In mixed β 1-4/1-3 d-glucopyranosides, as those found in oats and barley, the ratio of the β 1-4 and β 1-3 linked residues is crucial in determining the structural and dynamical attributes.
33572006	3	50	located	found	599:603	arg1	oats					608:611	oats	608:611	oats	608:611	In mixed β 1-4/1-3 d-glucopyranosides, as those found in oats and barley, the ratio of the β 1-4 and β 1-3 linked residues is crucial in determining the structural and dynamical attributes.
33572006	3	50	located	found	599:603	arg1	barley					617:622	barley	617:622	barley	617:622	In mixed β 1-4/1-3 d-glucopyranosides, as those found in oats and barley, the ratio of the β 1-4 and β 1-3 linked residues is crucial in determining the structural and dynamical attributes.
33572006	3	51	theme	structural	704:713	arg1	attributes					729:738	the structural and dynamical attributes	700:738	the structural and dynamical attributes	700:738	In mixed β 1-4/1-3 d-glucopyranosides, as those found in oats and barley, the ratio of the β 1-4 and β 1-3 linked residues is crucial in determining the structural and dynamical attributes.
33572006	2	52	theme	units	544:548	arg1	type					532:535	the composition, anomeric nature and glycosidic linkage type	476:535	the composition, anomeric nature and glycosidic linkage type of the units	476:548	Results indicate that the litheness of the oligosaccharide chain is noticeably controlled by the composition, anomeric nature and glycosidic linkage type of the units.
33572006	1	53	dep	one	269:271	arg1	linkages					306:313	glycosidic linkages	295:313	glycosidic linkages	295:313	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33572006	5	54	from	simulated	1054:1062	arg1	work					1072:1075	this work	1067:1075	this work	1067:1075	The properties simulated in this work are meant to provide a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties and are in line with some experimental structural attributes.
33572006	4	55	theme	angles	818:823	arg1	coordinates					795:805	the internal coordinates	782:805	the internal coordinates of torsion angles subtended by glycosidic oxygen atoms	782:860	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms and subsequent K-means clustering of the dynamical space spanned by PC1 to PC2 point to the dynamical and structural disparity in the various types of oligosaccharides studied.
33572006	1	56	theme	dynamical	361:369	arg1	properties					371:380	their structural and dynamical properties	340:380	their structural and dynamical properties	340:380	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33572006	1	57	theme	molecular	95:103	arg1	simulation					114:123	a molecular dynamics simulation	93:123	a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages	93:313	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33572006	1	58	theme	1-3/1-4	280:286	arg1	one					269:271	one	269:271	one	269:271	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33572006	1	58	theme	1-3/1-4	280:286	arg1	1-3/1-4					280:286	the 1-3/1-4	276:286	the 1-3/1-4	276:286	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33572006	4	59	theme	glycosidic	838:847	arg1	atoms					856:860	glycosidic oxygen atoms	838:860	glycosidic oxygen atoms	838:860	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms and subsequent K-means clustering of the dynamical space spanned by PC1 to PC2 point to the dynamical and structural disparity in the various types of oligosaccharides studied.
33572006	4	60	theme	dynamical	954:962	arg1	disparity					979:987	the dynamical and structural disparity	950:987	the dynamical and structural disparity in the various types of oligosaccharides studied	950:1036	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms and subsequent K-means clustering of the dynamical space spanned by PC1 to PC2 point to the dynamical and structural disparity in the various types of oligosaccharides studied.
33572006	4	61	theme	Principal	741:749	arg1	PCA					771:773	PCA	771:773	PCA	771:773	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms and subsequent K-means clustering of the dynamical space spanned by PC1 to PC2 point to the dynamical and structural disparity in the various types of oligosaccharides studied.
33572006	4	61	theme	Principal	741:749	arg1	analysis					761:768	Principal component analysis	741:768	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms	741:860	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms and subsequent K-means clustering of the dynamical space spanned by PC1 to PC2 point to the dynamical and structural disparity in the various types of oligosaccharides studied.
33572006	1	62	theme	dynamics	105:112	arg1	simulation					114:123	a molecular dynamics simulation	93:123	a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages	93:313	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33572006	4	63	theme	various	996:1002	arg1	types					1004:1008	the various types	992:1008	the various types of oligosaccharides studied	992:1036	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms and subsequent K-means clustering of the dynamical space spanned by PC1 to PC2 point to the dynamical and structural disparity in the various types of oligosaccharides studied.
33572006	1	64	theme	carbohydrate	206:217	arg1	block					197:201	the very basic building block	173:201	the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages	173:313	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33572006	3	65	link	linked	658:663	arg1	residues					665:672	the β 1-4 and β 1-3 linked residues	638:672	residues	665:672	In mixed β 1-4/1-3 d-glucopyranosides, as those found in oats and barley, the ratio of the β 1-4 and β 1-3 linked residues is crucial in determining the structural and dynamical attributes.
33572006	1	66	theme	simulation	114:123	arg1	study					125:129	a molecular dynamics simulation study	93:129	a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages	93:313	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33572006	4	67	theme	oligosaccharides	1013:1028	arg1	types					1004:1008	the various types	992:1008	the various types of oligosaccharides studied	992:1036	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms and subsequent K-means clustering of the dynamical space spanned by PC1 to PC2 point to the dynamical and structural disparity in the various types of oligosaccharides studied.
33572006	5	68	theme	linkage	1162:1168	arg1	importance					1148:1157	the importance	1144:1157	the importance of linkage and anomericity on the oligosaccharide chain properties	1144:1224	The properties simulated in this work are meant to provide a systematic yet comparative understanding of the importance of linkage and anomericity on the oligosaccharide chain properties and are in line with some experimental structural attributes.
33572006	0	69	theme	α-	63:64	arg1	structure					37:45	structure	37:45	structure	37:45	Relation between glycosidic linkage, structure and dynamics of α- and β-glucans in water.
33572006	0	69	theme	α-	63:64	arg1	linkage					28:34	glycosidic linkage	17:34	glycosidic linkage	17:34	Relation between glycosidic linkage, structure and dynamics of α- and β-glucans in water.
33572006	0	69	theme	α-	63:64	arg1	β-glucans					70:78	β-glucans	70:78	β-glucans in water	70:87	Relation between glycosidic linkage, structure and dynamics of α- and β-glucans in water.
33572006	0	69	theme	α-	63:64	arg1	dynamics					51:58	dynamics	51:58	dynamics	51:58	Relation between glycosidic linkage, structure and dynamics of α- and β-glucans in water.
33572006	3	70	theme	1-4/1-3	562:568	arg1	d-glucopyranosides					570:587	mixed β 1-4/1-3 d-glucopyranosides	554:587	mixed β 1-4/1-3 d-glucopyranosides	554:587	In mixed β 1-4/1-3 d-glucopyranosides, as those found in oats and barley, the ratio of the β 1-4 and β 1-3 linked residues is crucial in determining the structural and dynamical attributes.
33572006	1	71	dep	contrast	331:338	arg1	properties					371:380	their structural and dynamical properties	340:380	their structural and dynamical properties	340:380	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33572006	4	72	theme	dynamical	903:911	arg1	space					913:917	the dynamical space	899:917	the dynamical space spanned by PC1	899:932	Principal component analysis (PCA) using the internal coordinates of torsion angles subtended by glycosidic oxygen atoms and subsequent K-means clustering of the dynamical space spanned by PC1 to PC2 point to the dynamical and structural disparity in the various types of oligosaccharides studied.
33572006	0	73	from	linkage	28:34	arg1	water					83:87	water	83:87	water	83:87	Relation between glycosidic linkage, structure and dynamics of α- and β-glucans in water.
33572006	3	74	theme	dynamical	719:727	arg1	attributes					729:738	the structural and dynamical attributes	700:738	the structural and dynamical attributes	700:738	In mixed β 1-4/1-3 d-glucopyranosides, as those found in oats and barley, the ratio of the β 1-4 and β 1-3 linked residues is crucial in determining the structural and dynamical attributes.
33572006	1	75	theme	α-	224:225	arg1	carbohydrate					206:217	carbohydrate	206:217	carbohydrate	206:217	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33572006	1	75	theme	α-	224:225	arg1	units					248:252	the α- or β-d glucopyranose units	220:252	the α- or β-d glucopyranose units	220:252	In a molecular dynamics simulation study of several oligosaccharides comprising of the very basic building block of carbohydrate, the α- or β-d glucopyranose units, linked by any one of the 1-3/1-4 or 1-6 glycosidic linkages, we compare and contrast their structural and dynamical properties.
33775764	5	0	theme	ALP	798:800	arg1	staining					802:809	ALP staining	798:809	ALP staining	798:809	Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR).
33775764	6	1	theme	biological	913:922	arg1	results					935:941	The biological experiment results	909:941	The biological experiment results	909:941	The biological experiment results show that LP-Sr can effectively promote proliferation and increase the expression of VEGF and EGFL6 in HUVECs and significantly up-regulate the expression of Col1α1 and OCN in MC3T3-E1.
33775764	9	2	theme	polysaccharide	1459:1472	arg1	LP-Sr					1421:1425	LP-Sr	1421:1425	LP-Sr	1421:1425	Hence, we believe that LP-Sr should be a promising and novel polysaccharide complex in modulating osteogenesis-angiogenesis for bone regeneration.
33775764	9	2	theme	polysaccharide	1459:1472	arg1	complex					1474:1480	a promising and novel polysaccharide complex	1437:1480	a promising and novel polysaccharide complex	1437:1480	Hence, we believe that LP-Sr should be a promising and novel polysaccharide complex in modulating osteogenesis-angiogenesis for bone regeneration.
33775764	7	3	contain	has	1165:1167	arg1	LP-Sr					1159:1163	LP-Sr	1159:1163	LP-Sr	1159:1163	Besides, it is suggested that LP-Sr has positive effects on the suppression of pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1.
33775764	7	3	contain	has	1165:1167	arg2	effects					1178:1184	positive effects	1169:1184	positive effects	1169:1184	Besides, it is suggested that LP-Sr has positive effects on the suppression of pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1.
33775764	8	4	theme	high	1366:1369	arg1	expression					1371:1380	high expression	1366:1380	high expression in LP-Sr group	1366:1395	Moreover, the osteogenic and angiogenic markers, i.e. alkaline phosphatase (ALP) and CD31, exhibited high expression in LP-Sr group.
33775764	6	5	theme	EGFL6	1037:1041	arg1	expression					1014:1023	the expression	1010:1023	the expression of VEGF and EGFL6 in HUVECs	1010:1051	The biological experiment results show that LP-Sr can effectively promote proliferation and increase the expression of VEGF and EGFL6 in HUVECs and significantly up-regulate the expression of Col1α1 and OCN in MC3T3-E1.
33775764	7	6	from	suppression	1193:1203	arg1	MC3T3-E1					1255:1262	MC3T3-E1	1255:1262	MC3T3-E1	1255:1262	Besides, it is suggested that LP-Sr has positive effects on the suppression of pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1.
33775764	7	6	from	suppression	1193:1203	arg1	HUVECs					1244:1249	HUVECs	1244:1249	HUVECs	1244:1249	Besides, it is suggested that LP-Sr has positive effects on the suppression of pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1.
33775764	5	7	theme	biomedical	689:698	arg1	potential					700:708	the biomedical potential	685:708	the biomedical potential of this complex	685:724	Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR).
33775764	2	8	theme	novel	347:351	arg1	complex					374:380	a novel polysaccharide-metal complex	345:380	a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis	345:461	In this study, we synthesized strontium Laminarin polysaccharide (LP-Sr), a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis.
33775764	2	8	theme	novel	347:351	arg1	polysaccharide					321:334	strontium Laminarin polysaccharide	301:334	strontium Laminarin polysaccharide (LP-Sr)	301:342	In this study, we synthesized strontium Laminarin polysaccharide (LP-Sr), a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis.
33775764	1	9	theme	clinical	151:158	arg1	worldwide					171:179	the major clinical challenges worldwide	141:179	the major clinical challenges worldwide	141:179	Bone regeneration and repair has become one of the major clinical challenges worldwide and it involves multiple processes including inflammation, angiogenesis and osteogenesis.
33775764	5	10	theme	cell	772:775	arg1	assay					791:795	cell proliferation assay	772:795	cell proliferation assay	772:795	Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR).
33775764	2	11	contain	have	394:397	arg1	complex					374:380	a novel polysaccharide-metal complex	345:380	a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis	345:461	In this study, we synthesized strontium Laminarin polysaccharide (LP-Sr), a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis.
33775764	2	11	contain	have	394:397	arg2	effects					411:417	therapeutic effects	399:417	therapeutic effects	399:417	In this study, we synthesized strontium Laminarin polysaccharide (LP-Sr), a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis.
33775764	2	11	contain	have	394:397	arg1	polysaccharide					321:334	strontium Laminarin polysaccharide	301:334	strontium Laminarin polysaccharide (LP-Sr)	301:342	In this study, we synthesized strontium Laminarin polysaccharide (LP-Sr), a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis.
33775764	5	12	theme	CD31	842:845	arg1	staining					830:837	immunofluorescent staining	812:837	immunofluorescent staining of CD31	812:845	Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR).
33775764	5	12	theme	CD31	842:845	arg1	reaction					890:897	reverse transcription polymerase chain reaction	851:897	reverse transcription polymerase chain reaction (RT-PCR)	851:906	Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR).
33775764	5	12	theme	CD31	842:845	arg1	RT-PCR					900:905	RT-PCR	900:905	RT-PCR	900:905	Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR).
33775764	5	12	theme	CD31	842:845	arg1	assay					791:795	cell proliferation assay	772:795	cell proliferation assay	772:795	Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR).
33775764	5	12	theme	CD31	842:845	arg1	staining					802:809	ALP staining	798:809	ALP staining	798:809	Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR).
33775764	2	13	theme	Laminarin	311:319	arg1	polysaccharide					321:334	strontium Laminarin polysaccharide	301:334	strontium Laminarin polysaccharide (LP-Sr)	301:342	In this study, we synthesized strontium Laminarin polysaccharide (LP-Sr), a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis.
33775764	2	13	theme	Laminarin	311:319	arg1	complex					374:380	a novel polysaccharide-metal complex	345:380	a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis	345:461	In this study, we synthesized strontium Laminarin polysaccharide (LP-Sr), a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis.
33775764	2	13	theme	Laminarin	311:319	arg1	LP-Sr					337:341	LP-Sr	337:341	LP-Sr	337:341	In this study, we synthesized strontium Laminarin polysaccharide (LP-Sr), a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis.
33775764	6	14	theme	Col1α1	1101:1106	arg1	expression					1087:1096	the expression	1083:1096	the expression of Col1α1 and OCN in MC3T3-E1	1083:1126	The biological experiment results show that LP-Sr can effectively promote proliferation and increase the expression of VEGF and EGFL6 in HUVECs and significantly up-regulate the expression of Col1α1 and OCN in MC3T3-E1.
33775764	5	15	theme	proliferation	777:789	arg1	assay					791:795	cell proliferation assay	772:795	cell proliferation assay	772:795	Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR).
33775764	6	16	theme	experiment	924:933	arg1	results					935:941	The biological experiment results	909:941	The biological experiment results	909:941	The biological experiment results show that LP-Sr can effectively promote proliferation and increase the expression of VEGF and EGFL6 in HUVECs and significantly up-regulate the expression of Col1α1 and OCN in MC3T3-E1.
33775764	2	17	theme	strontium	301:309	arg1	polysaccharide					321:334	strontium Laminarin polysaccharide	301:334	strontium Laminarin polysaccharide (LP-Sr)	301:342	In this study, we synthesized strontium Laminarin polysaccharide (LP-Sr), a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis.
33775764	2	17	theme	strontium	301:309	arg1	complex					374:380	a novel polysaccharide-metal complex	345:380	a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis	345:461	In this study, we synthesized strontium Laminarin polysaccharide (LP-Sr), a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis.
33775764	2	17	theme	strontium	301:309	arg1	LP-Sr					337:341	LP-Sr	337:341	LP-Sr	337:341	In this study, we synthesized strontium Laminarin polysaccharide (LP-Sr), a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis.
33775764	1	18	theme	multiple	197:204	arg1	processes					206:214	multiple processes	197:214	multiple processes including inflammation, angiogenesis and osteogenesis	197:268	Bone regeneration and repair has become one of the major clinical challenges worldwide and it involves multiple processes including inflammation, angiogenesis and osteogenesis.
33775764	1	18	theme	multiple	197:204	arg1	osteogenesis					257:268	osteogenesis	257:268	osteogenesis	257:268	Bone regeneration and repair has become one of the major clinical challenges worldwide and it involves multiple processes including inflammation, angiogenesis and osteogenesis.
33775764	1	18	theme	multiple	197:204	arg1	angiogenesis					240:251	angiogenesis	240:251	angiogenesis	240:251	Bone regeneration and repair has become one of the major clinical challenges worldwide and it involves multiple processes including inflammation, angiogenesis and osteogenesis.
33775764	1	18	theme	multiple	197:204	arg1	inflammation					226:237	inflammation	226:237	inflammation	226:237	Bone regeneration and repair has become one of the major clinical challenges worldwide and it involves multiple processes including inflammation, angiogenesis and osteogenesis.
33775764	0	19	theme	Laminarin	10:18	arg1	polysaccharide					20:33	Strontium Laminarin polysaccharide	0:33	Strontium Laminarin polysaccharide	0:33	Strontium Laminarin polysaccharide modulates osteogenesis-angiogenesis for bone regeneration.
33775764	3	20	theme	1H	554:555	arg1	NMR					557:559	1H NMR	554:559	1H NMR	554:559	The structure and composition of the as-fabricated LP-Sr were analyzed by EDS, XRD, FITR, 1H NMR, HPLC, etc.
33775764	3	20	theme	1H	554:555	arg1	EDS					538:540	EDS	538:540	EDS	538:540	The structure and composition of the as-fabricated LP-Sr were analyzed by EDS, XRD, FITR, 1H NMR, HPLC, etc.
33775764	6	21	from	expression	1087:1096	arg1	MC3T3-E1					1119:1126	MC3T3-E1	1119:1126	MC3T3-E1	1119:1126	The biological experiment results show that LP-Sr can effectively promote proliferation and increase the expression of VEGF and EGFL6 in HUVECs and significantly up-regulate the expression of Col1α1 and OCN in MC3T3-E1.
33775764	7	22	theme	pro-inflammatory	1208:1223	arg1	IL6					1232:1234	pro-inflammatory factor IL6	1208:1234	pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1	1208:1262	Besides, it is suggested that LP-Sr has positive effects on the suppression of pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1.
33775764	9	23	theme	novel	1453:1457	arg1	LP-Sr					1421:1425	LP-Sr	1421:1425	LP-Sr	1421:1425	Hence, we believe that LP-Sr should be a promising and novel polysaccharide complex in modulating osteogenesis-angiogenesis for bone regeneration.
33775764	9	23	theme	novel	1453:1457	arg1	complex					1474:1480	a promising and novel polysaccharide complex	1437:1480	a promising and novel polysaccharide complex	1437:1480	Hence, we believe that LP-Sr should be a promising and novel polysaccharide complex in modulating osteogenesis-angiogenesis for bone regeneration.
33775764	0	24	theme	Strontium	0:8	arg1	polysaccharide					20:33	Strontium Laminarin polysaccharide	0:33	Strontium Laminarin polysaccharide	0:33	Strontium Laminarin polysaccharide modulates osteogenesis-angiogenesis for bone regeneration.
33775764	6	25	theme	VEGF	1028:1031	arg1	expression					1014:1023	the expression	1010:1023	the expression of VEGF and EGFL6 in HUVECs	1010:1051	The biological experiment results show that LP-Sr can effectively promote proliferation and increase the expression of VEGF and EGFL6 in HUVECs and significantly up-regulate the expression of Col1α1 and OCN in MC3T3-E1.
33775764	3	26	dep	structure	468:476	arg1	The					464:466	The	464:466	The	464:466	The structure and composition of the as-fabricated LP-Sr were analyzed by EDS, XRD, FITR, 1H NMR, HPLC, etc.
33775764	8	27	theme	LP-Sr	1385:1389	arg1	group					1391:1395	LP-Sr group	1385:1395	LP-Sr group	1385:1395	Moreover, the osteogenic and angiogenic markers, i.e. alkaline phosphatase (ALP) and CD31, exhibited high expression in LP-Sr group.
33775764	8	28	theme	angiogenic	1294:1303	arg1	markers					1305:1311	the osteogenic and angiogenic markers	1275:1311	the osteogenic and angiogenic markers	1275:1311	Moreover, the osteogenic and angiogenic markers, i.e. alkaline phosphatase (ALP) and CD31, exhibited high expression in LP-Sr group.
33775764	6	29	theme	OCN	1112:1114	arg1	expression					1087:1096	the expression	1083:1096	the expression of Col1α1 and OCN in MC3T3-E1	1083:1126	The biological experiment results show that LP-Sr can effectively promote proliferation and increase the expression of VEGF and EGFL6 in HUVECs and significantly up-regulate the expression of Col1α1 and OCN in MC3T3-E1.
33775764	5	30	theme	reverse	851:857	arg1	reaction					890:897	reverse transcription polymerase chain reaction	851:897	reverse transcription polymerase chain reaction (RT-PCR)	851:906	Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR).
33775764	5	30	theme	reverse	851:857	arg1	RT-PCR					900:905	RT-PCR	900:905	RT-PCR	900:905	Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR).
33775764	6	31	from	expression	1014:1023	arg1	HUVECs					1046:1051	HUVECs	1046:1051	HUVECs	1046:1051	The biological experiment results show that LP-Sr can effectively promote proliferation and increase the expression of VEGF and EGFL6 in HUVECs and significantly up-regulate the expression of Col1α1 and OCN in MC3T3-E1.
33775764	9	32	theme	promising	1439:1447	arg1	LP-Sr					1421:1425	LP-Sr	1421:1425	LP-Sr	1421:1425	Hence, we believe that LP-Sr should be a promising and novel polysaccharide complex in modulating osteogenesis-angiogenesis for bone regeneration.
33775764	9	32	theme	promising	1439:1447	arg1	complex					1474:1480	a promising and novel polysaccharide complex	1437:1480	a promising and novel polysaccharide complex	1437:1480	Hence, we believe that LP-Sr should be a promising and novel polysaccharide complex in modulating osteogenesis-angiogenesis for bone regeneration.
33775764	5	33	theme	polymerase	873:882	arg1	reaction					890:897	reverse transcription polymerase chain reaction	851:897	reverse transcription polymerase chain reaction (RT-PCR)	851:906	Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR).
33775764	5	33	theme	polymerase	873:882	arg1	RT-PCR					900:905	RT-PCR	900:905	RT-PCR	900:905	Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR).
33775764	5	34	theme	transcription	859:871	arg1	reaction					890:897	reverse transcription polymerase chain reaction	851:897	reverse transcription polymerase chain reaction (RT-PCR)	851:906	Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR).
33775764	5	34	theme	transcription	859:871	arg1	RT-PCR					900:905	RT-PCR	900:905	RT-PCR	900:905	Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR).
33775764	7	35	from	IL6	1232:1234	arg1	MC3T3-E1					1255:1262	MC3T3-E1	1255:1262	MC3T3-E1	1255:1262	Besides, it is suggested that LP-Sr has positive effects on the suppression of pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1.
33775764	7	35	from	IL6	1232:1234	arg1	HUVECs					1244:1249	HUVECs	1244:1249	HUVECs	1244:1249	Besides, it is suggested that LP-Sr has positive effects on the suppression of pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1.
33775764	5	36	theme	chain	884:888	arg1	reaction					890:897	reverse transcription polymerase chain reaction	851:897	reverse transcription polymerase chain reaction (RT-PCR)	851:906	Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR).
33775764	5	36	theme	chain	884:888	arg1	RT-PCR					900:905	RT-PCR	900:905	RT-PCR	900:905	Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR).
33775764	9	37	theme	bone	1526:1529	arg1	regeneration					1531:1542	bone regeneration	1526:1542	bone regeneration	1526:1542	Hence, we believe that LP-Sr should be a promising and novel polysaccharide complex in modulating osteogenesis-angiogenesis for bone regeneration.
33775764	7	38	theme	factor	1225:1230	arg1	IL6					1232:1234	pro-inflammatory factor IL6	1208:1234	pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1	1208:1262	Besides, it is suggested that LP-Sr has positive effects on the suppression of pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1.
33775764	8	39	theme	osteogenic	1279:1288	arg1	markers					1305:1311	the osteogenic and angiogenic markers	1275:1311	the osteogenic and angiogenic markers	1275:1311	Moreover, the osteogenic and angiogenic markers, i.e. alkaline phosphatase (ALP) and CD31, exhibited high expression in LP-Sr group.
33775764	3	40	theme	as-fabricated	501:513	arg1	LP-Sr					515:519	the as-fabricated LP-Sr	497:519	the as-fabricated LP-Sr	497:519	The structure and composition of the as-fabricated LP-Sr were analyzed by EDS, XRD, FITR, 1H NMR, HPLC, etc.
33775764	5	41	theme	immunofluorescent	812:828	arg1	staining					830:837	immunofluorescent staining	812:837	immunofluorescent staining of CD31	812:845	Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR).
33775764	3	42	theme	LP-Sr	515:519	arg1	composition					482:492	composition	482:492	composition	482:492	The structure and composition of the as-fabricated LP-Sr were analyzed by EDS, XRD, FITR, 1H NMR, HPLC, etc.
33775764	3	42	theme	LP-Sr	515:519	arg1	structure					468:476	structure	468:476	structure	468:476	The structure and composition of the as-fabricated LP-Sr were analyzed by EDS, XRD, FITR, 1H NMR, HPLC, etc.
33775764	7	43	theme	positive	1169:1176	arg1	effects					1178:1184	positive effects	1169:1184	positive effects	1169:1184	Besides, it is suggested that LP-Sr has positive effects on the suppression of pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1.
33775764	7	44	from	HUVECs	1244:1249	arg1	suppression					1193:1203	the suppression	1189:1203	the suppression of pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1	1189:1262	Besides, it is suggested that LP-Sr has positive effects on the suppression of pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1.
33775764	0	45	theme	bone	75:78	arg1	regeneration					80:91	bone regeneration	75:91	bone regeneration	75:91	Strontium Laminarin polysaccharide modulates osteogenesis-angiogenesis for bone regeneration.
33775764	1	46	theme	worldwide	171:179	arg1	worldwide					171:179	the major clinical challenges worldwide	141:179	the major clinical challenges worldwide	141:179	Bone regeneration and repair has become one of the major clinical challenges worldwide and it involves multiple processes including inflammation, angiogenesis and osteogenesis.
33775764	1	46	theme	worldwide	171:179	arg1	one					134:136	one	134:136	one	134:136	Bone regeneration and repair has become one of the major clinical challenges worldwide and it involves multiple processes including inflammation, angiogenesis and osteogenesis.
33775764	8	47	theme	alkaline	1319:1326	arg1	ALP					1341:1343	ALP	1341:1343	ALP	1341:1343	Moreover, the osteogenic and angiogenic markers, i.e. alkaline phosphatase (ALP) and CD31, exhibited high expression in LP-Sr group.
33775764	8	47	theme	alkaline	1319:1326	arg1	phosphatase					1328:1338	alkaline phosphatase	1319:1338	alkaline phosphatase (ALP)	1319:1344	Moreover, the osteogenic and angiogenic markers, i.e. alkaline phosphatase (ALP) and CD31, exhibited high expression in LP-Sr group.
33775764	7	48	from	MC3T3-E1	1255:1262	arg1	suppression					1193:1203	the suppression	1189:1203	the suppression of pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1	1189:1262	Besides, it is suggested that LP-Sr has positive effects on the suppression of pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1.
33775764	1	49	theme	major	145:149	arg1	worldwide					171:179	the major clinical challenges worldwide	141:179	the major clinical challenges worldwide	141:179	Bone regeneration and repair has become one of the major clinical challenges worldwide and it involves multiple processes including inflammation, angiogenesis and osteogenesis.
33775764	8	50	from	expression	1371:1380	arg1	group					1391:1395	LP-Sr group	1385:1395	LP-Sr group	1385:1395	Moreover, the osteogenic and angiogenic markers, i.e. alkaline phosphatase (ALP) and CD31, exhibited high expression in LP-Sr group.
33775764	5	51	theme	complex	718:724	arg1	potential					700:708	the biomedical potential	685:708	the biomedical potential of this complex	685:724	Moreover, we evaluated the biomedical potential of this complex in promoting osteogenesis and angiogenesis by cell proliferation assay, ALP staining, immunofluorescent staining of CD31 and reverse transcription polymerase chain reaction (RT-PCR).
33775764	1	52	dep	Bone	94:97	arg1	regeneration					99:110	regeneration	99:110	regeneration	99:110	Bone regeneration and repair has become one of the major clinical challenges worldwide and it involves multiple processes including inflammation, angiogenesis and osteogenesis.
33775764	1	52	dep	Bone	94:97	arg1	repair					116:121	repair	116:121	repair	116:121	Bone regeneration and repair has become one of the major clinical challenges worldwide and it involves multiple processes including inflammation, angiogenesis and osteogenesis.
33775764	2	53	theme	polysaccharide-metal	353:372	arg1	complex					374:380	a novel polysaccharide-metal complex	345:380	a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis	345:461	In this study, we synthesized strontium Laminarin polysaccharide (LP-Sr), a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis.
33775764	2	53	theme	polysaccharide-metal	353:372	arg1	polysaccharide					321:334	strontium Laminarin polysaccharide	301:334	strontium Laminarin polysaccharide (LP-Sr)	301:342	In this study, we synthesized strontium Laminarin polysaccharide (LP-Sr), a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis.
33775764	8	54	dep	phosphatase	1328:1338	arg1	i.e.					1314:1317	i.e.	1314:1317	i.e.	1314:1317	Moreover, the osteogenic and angiogenic markers, i.e. alkaline phosphatase (ALP) and CD31, exhibited high expression in LP-Sr group.
33775764	1	55	theme	challenges	160:169	arg1	worldwide					171:179	the major clinical challenges worldwide	141:179	the major clinical challenges worldwide	141:179	Bone regeneration and repair has become one of the major clinical challenges worldwide and it involves multiple processes including inflammation, angiogenesis and osteogenesis.
33775764	4	56	theme	polysaccharide	638:651	arg1	complex					653:659	this novel polysaccharide complex	627:659	this novel polysaccharide complex	627:659	The results indicate that we successfully synthesized this novel polysaccharide complex.
33775764	7	57	theme	IL6	1232:1234	arg1	suppression					1193:1203	the suppression	1189:1203	the suppression of pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1	1189:1262	Besides, it is suggested that LP-Sr has positive effects on the suppression of pro-inflammatory factor IL6 in both HUVECs and MC3T3-E1.
33775764	2	58	theme	therapeutic	399:409	arg1	effects					411:417	therapeutic effects	399:417	therapeutic effects	399:417	In this study, we synthesized strontium Laminarin polysaccharide (LP-Sr), a novel polysaccharide-metal complex that should have therapeutic effects on modulating osteogenesis and angiogenesis.
33775764	4	59	theme	novel	632:636	arg1	complex					653:659	this novel polysaccharide complex	627:659	this novel polysaccharide complex	627:659	The results indicate that we successfully synthesized this novel polysaccharide complex.
32719787	1	0	theme	asparagine	166:175	arg1	tools					241:245	indispensable tools	227:245	indispensable tools	227:245	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins.
32719787	1	0	theme	asparagine	166:175	arg1	EC					210:211	PNGases, N-glycanases, EC 3.5.1.52	187:220	EC	210:211	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins.
32719787	1	0	theme	asparagine	166:175	arg1	amidases					177:184	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases	128:184	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52)	128:221	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins.
32719787	0	1	theme	Rational	70:77	arg1	Exploration					79:89	the Rational Exploration	66:89	the Rational Exploration of Unstudied Acidobacterial Genomes	66:125	Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes.
32719787	4	2	theme	bacterial	668:676	arg1	species					678:684	the bacterial species	664:684	the bacterial species Dyella japonica	664:700	One candidate (originating from the bacterial species Dyella japonica) showed superior properties both in solution-phase and immobilized on amino-, epoxy- and nitrilotriacetate resins when compared to currently acidic available PNGases.
32719787	5	3	theme	good	974:977	arg1	stability					987:995	good storage stability	974:995	good storage stability	974:995	The high expression yield compared to a previously described PNGase H+, broad substrate specificity, and good storage stability of this novel N-glycanase makes it a valuable tool for the analysis of protein glycosylation.
32719787	0	4	theme	Unstudied	94:102	arg1	Genomes					119:125	Unstudied Acidobacterial Genomes	94:125	Unstudied Acidobacterial Genomes	94:125	Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes.
32719787	3	5	theme	pH	512:513	arg1	optimum					515:521	the acidic pH optimum	501:521	the acidic pH optimum of these acidobacterial PNGases	501:553	Herein, a panel of 13 novel PNGase H+ candidates (the suffix H+ refers to the acidic pH optimum of these acidobacterial PNGases) was tested in their recombinant form for their deglycosylation performance.
32719787	5	6	theme	substrate	947:955	arg1	specificity					957:967	broad substrate specificity	941:967	broad substrate specificity	941:967	The high expression yield compared to a previously described PNGase H+, broad substrate specificity, and good storage stability of this novel N-glycanase makes it a valuable tool for the analysis of protein glycosylation.
32719787	5	7	theme	glycosylation	1076:1088	arg1	analysis					1056:1063	the analysis	1052:1063	the analysis of protein glycosylation	1052:1088	The high expression yield compared to a previously described PNGase H+, broad substrate specificity, and good storage stability of this novel N-glycanase makes it a valuable tool for the analysis of protein glycosylation.
32719787	3	8	dep	candidates	465:474	arg1	refers					491:496	refers	491:496	refers to the acidic pH optimum of these acidobacterial PNGases	491:553	Herein, a panel of 13 novel PNGase H+ candidates (the suffix H+ refers to the acidic pH optimum of these acidobacterial PNGases) was tested in their recombinant form for their deglycosylation performance.
32719787	3	9	theme	suffix	481:486	arg1	H+					488:489	the suffix H+	477:489	the suffix H+	477:489	Herein, a panel of 13 novel PNGase H+ candidates (the suffix H+ refers to the acidic pH optimum of these acidobacterial PNGases) was tested in their recombinant form for their deglycosylation performance.
32719787	5	10	theme	PNGase	930:935	arg1	H+					937:938	a previously described PNGase H+	907:938	a previously described PNGase H+	907:938	The high expression yield compared to a previously described PNGase H+, broad substrate specificity, and good storage stability of this novel N-glycanase makes it a valuable tool for the analysis of protein glycosylation.
32719787	0	11	theme	Genomes	119:125	arg1	Exploration					79:89	the Rational Exploration	66:89	the Rational Exploration of Unstudied Acidobacterial Genomes	66:125	Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes.
32719787	2	12	gly	glycoproteins	386:398	arg1	glycoproteins					386:398	glycoproteins	386:398	glycoproteins	386:398	So far, only a limited number of PNGase candidates are available for the structural analysis of glycoproteins and their glycan moieties.
32719787	3	13	theme	PNGase	455:460	arg1	candidates					465:474	13 novel PNGase H+ candidates	446:474	13 novel PNGase H+ candidates (the suffix H+ refers to the acidic pH optimum of these acidobacterial PNGases)	446:554	Herein, a panel of 13 novel PNGase H+ candidates (the suffix H+ refers to the acidic pH optimum of these acidobacterial PNGases) was tested in their recombinant form for their deglycosylation performance.
32719787	5	14	theme	described	920:928	arg1	H+					937:938	a previously described PNGase H+	907:938	a previously described PNGase H+	907:938	The high expression yield compared to a previously described PNGase H+, broad substrate specificity, and good storage stability of this novel N-glycanase makes it a valuable tool for the analysis of protein glycosylation.
32719787	5	15	theme	protein	1068:1074	arg1	glycosylation					1076:1088	protein glycosylation	1068:1088	protein glycosylation	1068:1088	The high expression yield compared to a previously described PNGase H+, broad substrate specificity, and good storage stability of this novel N-glycanase makes it a valuable tool for the analysis of protein glycosylation.
32719787	0	16	theme	Acidobacterial	104:117	arg1	Genomes					119:125	Unstudied Acidobacterial Genomes	94:125	Unstudied Acidobacterial Genomes	94:125	Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes.
32719787	1	17	theme	indispensable	227:239	arg1	tools					241:245	indispensable tools	227:245	indispensable tools	227:245	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins.
32719787	1	17	theme	indispensable	227:239	arg1	amidases					177:184	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases	128:184	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52)	128:221	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins.
32719787	1	18	theme	PNGases	187:193	arg1	EC					210:211	PNGases, N-glycanases, EC 3.5.1.52	187:220	EC	210:211	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins.
32719787	1	18	theme	PNGases	187:193	arg1	N-glycanases					196:207	N-glycanases	196:207	N-glycanases	196:207	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins.
32719787	1	18	theme	PNGases	187:193	arg1	amidases					177:184	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases	128:184	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52)	128:221	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins.
32719787	4	19	theme	available	850:858	arg1	PNGases					860:866	currently acidic available PNGases	833:866	currently acidic available PNGases	833:866	One candidate (originating from the bacterial species Dyella japonica) showed superior properties both in solution-phase and immobilized on amino-, epoxy- and nitrilotriacetate resins when compared to currently acidic available PNGases.
32719787	3	20	theme	acidobacterial	532:545	arg1	PNGases					547:553	these acidobacterial PNGases	526:553	these acidobacterial PNGases	526:553	Herein, a panel of 13 novel PNGase H+ candidates (the suffix H+ refers to the acidic pH optimum of these acidobacterial PNGases) was tested in their recombinant form for their deglycosylation performance.
32719787	4	21	dep	properties	719:728	arg1	both					730:733	both	730:733	both	730:733	One candidate (originating from the bacterial species Dyella japonica) showed superior properties both in solution-phase and immobilized on amino-, epoxy- and nitrilotriacetate resins when compared to currently acidic available PNGases.
32719787	4	21	dep	properties	719:728	arg1	immobilized					757:767	immobilized	757:767	immobilized on amino-, epoxy- and nitrilotriacetate resins when compared to currently acidic available PNGases	757:866	One candidate (originating from the bacterial species Dyella japonica) showed superior properties both in solution-phase and immobilized on amino-, epoxy- and nitrilotriacetate resins when compared to currently acidic available PNGases.
32719787	1	22	gly	glycoproteins	275:287	arg1	glycoproteins					275:287	glycoproteins	275:287	glycoproteins	275:287	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins.
32719787	2	23	theme	limited	305:311	arg1	available					345:353	available	345:353	available	345:353	So far, only a limited number of PNGase candidates are available for the structural analysis of glycoproteins and their glycan moieties.
32719787	2	23	theme	limited	305:311	arg1	number					313:318	only a limited number	298:318	only a limited number of PNGase candidates	298:339	So far, only a limited number of PNGase candidates are available for the structural analysis of glycoproteins and their glycan moieties.
32719787	4	24	dep	amino-	772:777	arg1	resins					809:814	resins	809:814	resins	809:814	One candidate (originating from the bacterial species Dyella japonica) showed superior properties both in solution-phase and immobilized on amino-, epoxy- and nitrilotriacetate resins when compared to currently acidic available PNGases.
32719787	0	25	theme	Active	20:25	arg1	Variants					49:56	Highly Active Recombinant PNGase H+ Variants	13:56	Highly Active Recombinant PNGase H+ Variants	13:56	Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes.
32719787	4	26	theme	superior	710:717	arg1	properties					719:728	superior properties	710:728	superior properties both in solution-phase and immobilized on amino-, epoxy- and nitrilotriacetate resins when compared to currently acidic available PNGases	710:866	One candidate (originating from the bacterial species Dyella japonica) showed superior properties both in solution-phase and immobilized on amino-, epoxy- and nitrilotriacetate resins when compared to currently acidic available PNGases.
32719787	5	27	theme	broad	941:945	arg1	specificity					957:967	broad substrate specificity	941:967	broad substrate specificity	941:967	The high expression yield compared to a previously described PNGase H+, broad substrate specificity, and good storage stability of this novel N-glycanase makes it a valuable tool for the analysis of protein glycosylation.
32719787	3	28	theme	novel	449:453	arg1	candidates					465:474	13 novel PNGase H+ candidates	446:474	13 novel PNGase H+ candidates (the suffix H+ refers to the acidic pH optimum of these acidobacterial PNGases)	446:554	Herein, a panel of 13 novel PNGase H+ candidates (the suffix H+ refers to the acidic pH optimum of these acidobacterial PNGases) was tested in their recombinant form for their deglycosylation performance.
32719787	3	29	theme	PNGases	547:553	arg1	optimum					515:521	the acidic pH optimum	501:521	the acidic pH optimum of these acidobacterial PNGases	501:553	Herein, a panel of 13 novel PNGase H+ candidates (the suffix H+ refers to the acidic pH optimum of these acidobacterial PNGases) was tested in their recombinant form for their deglycosylation performance.
32719787	1	30	from	glycoproteins	275:287	arg1	N-glycans					260:268	N-glycans	260:268	N-glycans from glycoproteins	260:287	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins.
32719787	4	31	dep	Dyella	686:691	arg1	japonica					693:700	japonica	693:700	japonica	693:700	One candidate (originating from the bacterial species Dyella japonica) showed superior properties both in solution-phase and immobilized on amino-, epoxy- and nitrilotriacetate resins when compared to currently acidic available PNGases.
32719787	2	32	theme	moieties	417:424	arg1	analysis					374:381	the structural analysis	359:381	the structural analysis of glycoproteins and their glycan moieties	359:424	So far, only a limited number of PNGase candidates are available for the structural analysis of glycoproteins and their glycan moieties.
32719787	0	33	theme	PNGase	39:44	arg1	Variants					49:56	Highly Active Recombinant PNGase H+ Variants	13:56	Highly Active Recombinant PNGase H+ Variants	13:56	Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes.
32719787	2	34	theme	candidates	330:339	arg1	available					345:353	available	345:353	available	345:353	So far, only a limited number of PNGase candidates are available for the structural analysis of glycoproteins and their glycan moieties.
32719787	2	34	theme	candidates	330:339	arg1	number					313:318	only a limited number	298:318	only a limited number of PNGase candidates	298:339	So far, only a limited number of PNGase candidates are available for the structural analysis of glycoproteins and their glycan moieties.
32719787	5	35	theme	novel	1005:1009	arg1	N-glycanase					1011:1021	this novel N-glycanase	1000:1021	this novel N-glycanase	1000:1021	The high expression yield compared to a previously described PNGase H+, broad substrate specificity, and good storage stability of this novel N-glycanase makes it a valuable tool for the analysis of protein glycosylation.
32719787	5	36	theme	high	873:876	arg1	yield					889:893	The high expression yield	869:893	The high expression yield compared to a previously described PNGase H+, broad substrate specificity, and good storage stability of this novel N-glycanase	869:1021	The high expression yield compared to a previously described PNGase H+, broad substrate specificity, and good storage stability of this novel N-glycanase makes it a valuable tool for the analysis of protein glycosylation.
32719787	2	37	theme	glycan	410:415	arg1	moieties					417:424	their glycan moieties	404:424	their glycan moieties	404:424	So far, only a limited number of PNGase candidates are available for the structural analysis of glycoproteins and their glycan moieties.
32719787	0	38	theme	Recombinant	27:37	arg1	Variants					49:56	Highly Active Recombinant PNGase H+ Variants	13:56	Highly Active Recombinant PNGase H+ Variants	13:56	Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes.
32719787	5	39	theme	N-glycanase	1011:1021	arg1	H+					937:938	a previously described PNGase H+	907:938	a previously described PNGase H+	907:938	The high expression yield compared to a previously described PNGase H+, broad substrate specificity, and good storage stability of this novel N-glycanase makes it a valuable tool for the analysis of protein glycosylation.
32719787	5	39	theme	N-glycanase	1011:1021	arg1	stability					987:995	good storage stability	974:995	good storage stability	974:995	The high expression yield compared to a previously described PNGase H+, broad substrate specificity, and good storage stability of this novel N-glycanase makes it a valuable tool for the analysis of protein glycosylation.
32719787	5	39	theme	N-glycanase	1011:1021	arg1	specificity					957:967	broad substrate specificity	941:967	broad substrate specificity	941:967	The high expression yield compared to a previously described PNGase H+, broad substrate specificity, and good storage stability of this novel N-glycanase makes it a valuable tool for the analysis of protein glycosylation.
32719787	0	40	theme	Variants	49:56	arg1	Discovery					0:8	Discovery	0:8	Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes.	0:126	Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes.
32719787	5	41	theme	expression	878:887	arg1	yield					889:893	The high expression yield	869:893	The high expression yield compared to a previously described PNGase H+, broad substrate specificity, and good storage stability of this novel N-glycanase	869:1021	The high expression yield compared to a previously described PNGase H+, broad substrate specificity, and good storage stability of this novel N-glycanase makes it a valuable tool for the analysis of protein glycosylation.
32719787	3	42	theme	deglycosylation	603:617	arg1	performance					619:629	their deglycosylation performance	597:629	their deglycosylation performance	597:629	Herein, a panel of 13 novel PNGase H+ candidates (the suffix H+ refers to the acidic pH optimum of these acidobacterial PNGases) was tested in their recombinant form for their deglycosylation performance.
32719787	0	43	theme	H+	46:47	arg1	Variants					49:56	Highly Active Recombinant PNGase H+ Variants	13:56	Highly Active Recombinant PNGase H+ Variants	13:56	Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes.
32719787	3	44	theme	H+	462:463	arg1	candidates					465:474	13 novel PNGase H+ candidates	446:474	13 novel PNGase H+ candidates (the suffix H+ refers to the acidic pH optimum of these acidobacterial PNGases)	446:554	Herein, a panel of 13 novel PNGase H+ candidates (the suffix H+ refers to the acidic pH optimum of these acidobacterial PNGases) was tested in their recombinant form for their deglycosylation performance.
32719787	1	45	theme	Peptide-N	128:136	arg1	tools					241:245	indispensable tools	227:245	indispensable tools	227:245	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins.
32719787	1	45	theme	Peptide-N	128:136	arg1	EC					210:211	PNGases, N-glycanases, EC 3.5.1.52	187:220	EC	210:211	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins.
32719787	1	45	theme	Peptide-N	128:136	arg1	amidases					177:184	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases	128:184	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52)	128:221	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins.
32719787	5	46	theme	valuable	1034:1041	arg1	tool					1043:1046	a valuable tool	1032:1046	a valuable tool for the analysis of protein glycosylation	1032:1088	The high expression yield compared to a previously described PNGase H+, broad substrate specificity, and good storage stability of this novel N-glycanase makes it a valuable tool for the analysis of protein glycosylation.
32719787	5	47	theme	storage	979:985	arg1	stability					987:995	good storage stability	974:995	good storage stability	974:995	The high expression yield compared to a previously described PNGase H+, broad substrate specificity, and good storage stability of this novel N-glycanase makes it a valuable tool for the analysis of protein glycosylation.
32719787	1	48	theme	4-	138:139	arg1	tools					241:245	indispensable tools	227:245	indispensable tools	227:245	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins.
32719787	1	48	theme	4-	138:139	arg1	EC					210:211	PNGases, N-glycanases, EC 3.5.1.52	187:220	EC	210:211	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins.
32719787	1	48	theme	4-	138:139	arg1	amidases					177:184	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases	128:184	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52)	128:221	Peptide-N 4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins.
32719787	3	49	theme	acidic	505:510	arg1	optimum					515:521	the acidic pH optimum	501:521	the acidic pH optimum of these acidobacterial PNGases	501:553	Herein, a panel of 13 novel PNGase H+ candidates (the suffix H+ refers to the acidic pH optimum of these acidobacterial PNGases) was tested in their recombinant form for their deglycosylation performance.
32719787	3	50	theme	candidates	465:474	arg1	panel					437:441	a panel	435:441	a panel of 13 novel PNGase H+ candidates (the suffix H+ refers to the acidic pH optimum of these acidobacterial PNGases)	435:554	Herein, a panel of 13 novel PNGase H+ candidates (the suffix H+ refers to the acidic pH optimum of these acidobacterial PNGases) was tested in their recombinant form for their deglycosylation performance.
32719787	2	51	theme	PNGase	323:328	arg1	candidates					330:339	PNGase candidates	323:339	PNGase candidates	323:339	So far, only a limited number of PNGase candidates are available for the structural analysis of glycoproteins and their glycan moieties.
32719787	4	52	dep	both	730:733	arg1	solution-phase					738:751	solution-phase	738:751	solution-phase	738:751	One candidate (originating from the bacterial species Dyella japonica) showed superior properties both in solution-phase and immobilized on amino-, epoxy- and nitrilotriacetate resins when compared to currently acidic available PNGases.
32719787	2	53	theme	structural	363:372	arg1	analysis					374:381	the structural analysis	359:381	the structural analysis of glycoproteins and their glycan moieties	359:424	So far, only a limited number of PNGase candidates are available for the structural analysis of glycoproteins and their glycan moieties.
32719787	2	54	theme	glycoproteins	386:398	arg1	analysis					374:381	the structural analysis	359:381	the structural analysis of glycoproteins and their glycan moieties	359:424	So far, only a limited number of PNGase candidates are available for the structural analysis of glycoproteins and their glycan moieties.
32719787	3	55	theme	recombinant	576:586	arg1	form					588:591	their recombinant form	570:591	their recombinant form	570:591	Herein, a panel of 13 novel PNGase H+ candidates (the suffix H+ refers to the acidic pH optimum of these acidobacterial PNGases) was tested in their recombinant form for their deglycosylation performance.
32719787	4	56	theme	Dyella	686:691	arg1	species					678:684	the bacterial species	664:684	the bacterial species Dyella japonica	664:700	One candidate (originating from the bacterial species Dyella japonica) showed superior properties both in solution-phase and immobilized on amino-, epoxy- and nitrilotriacetate resins when compared to currently acidic available PNGases.
31960003	1	0	contain	having	148:153	arg2	capabilities					213:224	multifunctional capabilities	197:224	multifunctional capabilities	197:224	Sustainably made, flexible and biocompatible composites, having environmentally friendly compositions and multifunctional capabilities, are promising materials for several emerging biomedical applications.
31960003	1	0	contain	having	148:153	arg1	composites					136:145	Sustainably made, flexible and biocompatible composites	91:145	Sustainably made, flexible and biocompatible composites	91:145	Sustainably made, flexible and biocompatible composites, having environmentally friendly compositions and multifunctional capabilities, are promising materials for several emerging biomedical applications.
31960003	1	0	contain	having	148:153	arg2	compositions					180:191	environmentally friendly compositions	155:191	environmentally friendly compositions	155:191	Sustainably made, flexible and biocompatible composites, having environmentally friendly compositions and multifunctional capabilities, are promising materials for several emerging biomedical applications.
31960003	1	0	contain	having	148:153	arg1	materials					241:249	promising materials	231:249	promising materials for several emerging biomedical applications	231:294	Sustainably made, flexible and biocompatible composites, having environmentally friendly compositions and multifunctional capabilities, are promising materials for several emerging biomedical applications.
31960003	5	1	theme	higher	752:757	arg1	activity					732:739	The antioxidant activity	716:739	The antioxidant activity	716:739	The antioxidant activity is 9 times higher than that of pristine chitosan.
31960003	5	1	theme	higher	752:757	arg1	times					746:750	9 times	744:750	9 times higher than that of pristine chitosan	744:788	The antioxidant activity is 9 times higher than that of pristine chitosan.
31960003	6	2	theme	films	824:828	arg1	properties					806:815	The mechanical properties	791:815	The mechanical properties of the films	791:828	The mechanical properties of the films can be tuned from elastic (∼8 MPa) chitosan films to stiff (∼285 MPa) bionanocomposite films with 50% filler.
31960003	6	2	theme	films	824:828	arg1	tuned					837:841	tuned	837:841	tuned	837:841	The mechanical properties of the films can be tuned from elastic (∼8 MPa) chitosan films to stiff (∼285 MPa) bionanocomposite films with 50% filler.
31960003	7	3	theme	hyperthermia	953:964	arg1	tests					966:970	The magnetic hyperthermia tests	940:970	The magnetic hyperthermia tests	940:970	The magnetic hyperthermia tests showed a temperature increase of 40 °C in 45 s for the 50% rGO-Fe3-xO4 film.
31960003	1	4	theme	multifunctional	197:211	arg1	capabilities					213:224	multifunctional capabilities	197:224	multifunctional capabilities	197:224	Sustainably made, flexible and biocompatible composites, having environmentally friendly compositions and multifunctional capabilities, are promising materials for several emerging biomedical applications.
31960003	2	5	with	bionanocomposites	366:382	arg1	filler					445:450	a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler	389:450	a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler	389:450	Here, the development of flexible and multifunctional chitosan-based bionanocomposites with a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler is described.
31960003	7	6	theme	°C	1008:1009	arg1	increase					993:1000	a temperature increase	979:1000	a temperature increase of 40 °C in 45 s	979:1017	The magnetic hyperthermia tests showed a temperature increase of 40 °C in 45 s for the 50% rGO-Fe3-xO4 film.
31960003	2	7	theme	chitosan-based	351:364	arg1	bionanocomposites					366:382	flexible and multifunctional chitosan-based bionanocomposites	322:382	flexible and multifunctional chitosan-based bionanocomposites with a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler	322:450	Here, the development of flexible and multifunctional chitosan-based bionanocomposites with a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler is described.
31960003	7	8	theme	50	1027:1028	arg1	%					1029:1029	%	1029:1029	%	1029:1029	The magnetic hyperthermia tests showed a temperature increase of 40 °C in 45 s for the 50% rGO-Fe3-xO4 film.
31960003	4	9	theme	room	698:701	arg1	temperature					703:713	room temperature	698:713	room temperature	698:713	The resulting bionanocomposites present superparamagnetic response at room temperature.
31960003	3	10	dep	nanoparticles	555:567	arg1	the					543:545	the	543:545	the	543:545	The filler is prepared by one-pot synthesis, ensuring good dispersibility of the Fe3-xO4 nanoparticles and rGO within the chitosan matrix during solvent casting.
31960003	6	11	theme	stiff	883:887	arg1	films					917:921	stiff (∼285 MPa) bionanocomposite films	883:921	stiff (∼285 MPa) bionanocomposite films with 50% filler	883:937	The mechanical properties of the films can be tuned from elastic (∼8 MPa) chitosan films to stiff (∼285 MPa) bionanocomposite films with 50% filler.
31960003	2	12	theme	multifunctional	335:349	arg1	bionanocomposites					366:382	flexible and multifunctional chitosan-based bionanocomposites	322:382	flexible and multifunctional chitosan-based bionanocomposites with a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler	322:450	Here, the development of flexible and multifunctional chitosan-based bionanocomposites with a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler is described.
31960003	2	13	theme	reduced	397:403	arg1	rGO-Fe3-xO4					432:442	rGO-Fe3-xO4	432:442	rGO-Fe3-xO4	432:442	Here, the development of flexible and multifunctional chitosan-based bionanocomposites with a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler is described.
31960003	2	13	theme	reduced	397:403	arg1	oxide					425:429	reduced graphene oxide-iron oxide	397:429	a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler	389:450	Here, the development of flexible and multifunctional chitosan-based bionanocomposites with a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler is described.
31960003	6	14	with	films	917:921	arg1	filler					932:937	50% filler	928:937	50% filler	928:937	The mechanical properties of the films can be tuned from elastic (∼8 MPa) chitosan films to stiff (∼285 MPa) bionanocomposite films with 50% filler.
31960003	7	15	from	increase	993:1000	arg1	s					1017:1017	45 s	1014:1017	45 s	1014:1017	The magnetic hyperthermia tests showed a temperature increase of 40 °C in 45 s for the 50% rGO-Fe3-xO4 film.
31960003	5	16	theme	antioxidant	720:730	arg1	activity					732:739	The antioxidant activity	716:739	The antioxidant activity	716:739	The antioxidant activity is 9 times higher than that of pristine chitosan.
31960003	5	16	theme	antioxidant	720:730	arg1	times					746:750	9 times	744:750	9 times higher than that of pristine chitosan	744:788	The antioxidant activity is 9 times higher than that of pristine chitosan.
31960003	2	17	theme	flexible	322:329	arg1	bionanocomposites					366:382	flexible and multifunctional chitosan-based bionanocomposites	322:382	flexible and multifunctional chitosan-based bionanocomposites with a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler	322:450	Here, the development of flexible and multifunctional chitosan-based bionanocomposites with a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler is described.
31960003	4	18	attach	present	660:666	arg2	bionanocomposites					642:658	The resulting bionanocomposites	628:658	The resulting bionanocomposites	628:658	The resulting bionanocomposites present superparamagnetic response at room temperature.
31960003	4	18	attach	present	660:666	arg1	temperature					703:713	room temperature	698:713	room temperature	698:713	The resulting bionanocomposites present superparamagnetic response at room temperature.
31960003	0	19	theme	chitosan-based	14:27	arg1	composites					29:38	Biocompatible chitosan-based composites	0:38	Biocompatible chitosan-based composites with properties suitable for hyperthermia therapy	0:88	Biocompatible chitosan-based composites with properties suitable for hyperthermia therapy.
31960003	3	20	theme	solvent	611:617	arg1	casting					619:625	solvent casting	611:625	solvent casting	611:625	The filler is prepared by one-pot synthesis, ensuring good dispersibility of the Fe3-xO4 nanoparticles and rGO within the chitosan matrix during solvent casting.
31960003	8	21	theme	nontumorigenic	1105:1118	arg1	line					1133:1136	the nontumorigenic (HaCat) cell line	1101:1136	the nontumorigenic (HaCat) cell line	1101:1136	Furthermore, the composites have no cytotoxicity to the nontumorigenic (HaCat) cell line, which confirms their biocompatibility and highlights the potential of these materials for biomedical applications, such as hyperthermia treatments.
31960003	6	22	theme	chitosan	865:872	arg1	MPa					860:862	∼8 MPa	857:862	∼8 MPa	857:862	The mechanical properties of the films can be tuned from elastic (∼8 MPa) chitosan films to stiff (∼285 MPa) bionanocomposite films with 50% filler.
31960003	6	22	theme	chitosan	865:872	arg1	films					874:878	elastic (∼8 MPa) chitosan films	848:878	elastic (∼8 MPa) chitosan films	848:878	The mechanical properties of the films can be tuned from elastic (∼8 MPa) chitosan films to stiff (∼285 MPa) bionanocomposite films with 50% filler.
31960003	2	23	theme	mixed	391:395	arg1	filler					445:450	a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler	389:450	a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler	389:450	Here, the development of flexible and multifunctional chitosan-based bionanocomposites with a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler is described.
31960003	6	24	theme	mechanical	795:804	arg1	properties					806:815	The mechanical properties	791:815	The mechanical properties of the films	791:828	The mechanical properties of the films can be tuned from elastic (∼8 MPa) chitosan films to stiff (∼285 MPa) bionanocomposite films with 50% filler.
31960003	6	24	theme	mechanical	795:804	arg1	tuned					837:841	tuned	837:841	tuned	837:841	The mechanical properties of the films can be tuned from elastic (∼8 MPa) chitosan films to stiff (∼285 MPa) bionanocomposite films with 50% filler.
31960003	3	25	theme	one-pot	492:498	arg1	synthesis					500:508	one-pot synthesis	492:508	one-pot synthesis	492:508	The filler is prepared by one-pot synthesis, ensuring good dispersibility of the Fe3-xO4 nanoparticles and rGO within the chitosan matrix during solvent casting.
31960003	0	26	theme	Biocompatible	0:12	arg1	composites					29:38	Biocompatible chitosan-based composites	0:38	Biocompatible chitosan-based composites with properties suitable for hyperthermia therapy	0:88	Biocompatible chitosan-based composites with properties suitable for hyperthermia therapy.
31960003	4	27	theme	resulting	632:640	arg1	bionanocomposites					642:658	The resulting bionanocomposites	628:658	The resulting bionanocomposites	628:658	The resulting bionanocomposites present superparamagnetic response at room temperature.
31960003	6	28	dep	stiff	883:887	arg1	MPa					895:897	∼285 MPa	890:897	∼285 MPa	890:897	The mechanical properties of the films can be tuned from elastic (∼8 MPa) chitosan films to stiff (∼285 MPa) bionanocomposite films with 50% filler.
31960003	1	29	theme	promising	231:239	arg1	composites					136:145	Sustainably made, flexible and biocompatible composites	91:145	Sustainably made, flexible and biocompatible composites	91:145	Sustainably made, flexible and biocompatible composites, having environmentally friendly compositions and multifunctional capabilities, are promising materials for several emerging biomedical applications.
31960003	1	29	theme	promising	231:239	arg1	materials					241:249	promising materials	231:249	promising materials for several emerging biomedical applications	231:294	Sustainably made, flexible and biocompatible composites, having environmentally friendly compositions and multifunctional capabilities, are promising materials for several emerging biomedical applications.
31960003	7	30	theme	magnetic	944:951	arg1	tests					966:970	The magnetic hyperthermia tests	940:970	The magnetic hyperthermia tests	940:970	The magnetic hyperthermia tests showed a temperature increase of 40 °C in 45 s for the 50% rGO-Fe3-xO4 film.
31960003	8	31	contain	have	1077:1080	arg1	composites					1066:1075	the composites	1062:1075	the composites	1062:1075	Furthermore, the composites have no cytotoxicity to the nontumorigenic (HaCat) cell line, which confirms their biocompatibility and highlights the potential of these materials for biomedical applications, such as hyperthermia treatments.
31960003	8	31	contain	have	1077:1080	arg2	cytotoxicity					1085:1096	no cytotoxicity	1082:1096	no cytotoxicity	1082:1096	Furthermore, the composites have no cytotoxicity to the nontumorigenic (HaCat) cell line, which confirms their biocompatibility and highlights the potential of these materials for biomedical applications, such as hyperthermia treatments.
31960003	8	32	theme	biomedical	1229:1238	arg1	treatments					1275:1284	hyperthermia treatments	1262:1284	hyperthermia treatments	1262:1284	Furthermore, the composites have no cytotoxicity to the nontumorigenic (HaCat) cell line, which confirms their biocompatibility and highlights the potential of these materials for biomedical applications, such as hyperthermia treatments.
31960003	8	32	theme	biomedical	1229:1238	arg1	applications					1240:1251	biomedical applications	1229:1251	biomedical applications	1229:1251	Furthermore, the composites have no cytotoxicity to the nontumorigenic (HaCat) cell line, which confirms their biocompatibility and highlights the potential of these materials for biomedical applications, such as hyperthermia treatments.
31960003	2	33	theme	bionanocomposites	366:382	arg1	development					307:317	the development	303:317	the development of flexible and multifunctional chitosan-based bionanocomposites with a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler	303:450	Here, the development of flexible and multifunctional chitosan-based bionanocomposites with a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler is described.
31960003	3	34	theme	chitosan	588:595	arg1	matrix					597:602	the chitosan matrix	584:602	the chitosan matrix during solvent casting	584:625	The filler is prepared by one-pot synthesis, ensuring good dispersibility of the Fe3-xO4 nanoparticles and rGO within the chitosan matrix during solvent casting.
31960003	0	35	theme	suitable	56:63	arg1	properties					45:54	properties	45:54	properties suitable for hyperthermia therapy	45:88	Biocompatible chitosan-based composites with properties suitable for hyperthermia therapy.
31960003	1	36	dep	made	103:106	arg1	flexible					109:116	flexible	109:116	flexible	109:116	Sustainably made, flexible and biocompatible composites, having environmentally friendly compositions and multifunctional capabilities, are promising materials for several emerging biomedical applications.
31960003	1	36	dep	made	103:106	arg1	biocompatible					122:134	biocompatible	122:134	biocompatible	122:134	Sustainably made, flexible and biocompatible composites, having environmentally friendly compositions and multifunctional capabilities, are promising materials for several emerging biomedical applications.
31960003	8	37	theme	cell	1128:1131	arg1	line					1133:1136	the nontumorigenic (HaCat) cell line	1101:1136	the nontumorigenic (HaCat) cell line	1101:1136	Furthermore, the composites have no cytotoxicity to the nontumorigenic (HaCat) cell line, which confirms their biocompatibility and highlights the potential of these materials for biomedical applications, such as hyperthermia treatments.
31960003	3	38	theme	good	520:523	arg1	dispersibility					525:538	good dispersibility	520:538	good dispersibility of the Fe3-xO4 nanoparticles and rGO within the chitosan matrix during solvent casting	520:625	The filler is prepared by one-pot synthesis, ensuring good dispersibility of the Fe3-xO4 nanoparticles and rGO within the chitosan matrix during solvent casting.
31960003	1	39	theme	several	255:261	arg1	applications					283:294	several emerging biomedical applications	255:294	several emerging biomedical applications	255:294	Sustainably made, flexible and biocompatible composites, having environmentally friendly compositions and multifunctional capabilities, are promising materials for several emerging biomedical applications.
31960003	4	40	theme	superparamagnetic	668:684	arg1	response					686:693	superparamagnetic response	668:693	superparamagnetic response	668:693	The resulting bionanocomposites present superparamagnetic response at room temperature.
31960003	6	41	theme	50	928:929	arg1	%					930:930	%	930:930	%	930:930	The mechanical properties of the films can be tuned from elastic (∼8 MPa) chitosan films to stiff (∼285 MPa) bionanocomposite films with 50% filler.
31960003	1	42	theme	emerging	263:270	arg1	applications					283:294	several emerging biomedical applications	255:294	several emerging biomedical applications	255:294	Sustainably made, flexible and biocompatible composites, having environmentally friendly compositions and multifunctional capabilities, are promising materials for several emerging biomedical applications.
31960003	0	43	theme	hyperthermia	69:80	arg1	therapy					82:88	hyperthermia therapy	69:88	hyperthermia therapy	69:88	Biocompatible chitosan-based composites with properties suitable for hyperthermia therapy.
31960003	5	44	theme	pristine	772:779	arg1	chitosan					781:788	pristine chitosan	772:788	pristine chitosan	772:788	The antioxidant activity is 9 times higher than that of pristine chitosan.
31960003	0	45	with	composites	29:38	arg1	properties					45:54	properties	45:54	properties suitable for hyperthermia therapy	45:88	Biocompatible chitosan-based composites with properties suitable for hyperthermia therapy.
31960003	2	46	theme	oxide	425:429	arg1	filler					445:450	a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler	389:450	a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler	389:450	Here, the development of flexible and multifunctional chitosan-based bionanocomposites with a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler is described.
31960003	8	47	theme	HaCat	1121:1125	arg1	line					1133:1136	the nontumorigenic (HaCat) cell line	1101:1136	the nontumorigenic (HaCat) cell line	1101:1136	Furthermore, the composites have no cytotoxicity to the nontumorigenic (HaCat) cell line, which confirms their biocompatibility and highlights the potential of these materials for biomedical applications, such as hyperthermia treatments.
31960003	6	48	theme	elastic	848:854	arg1	MPa					860:862	∼8 MPa	857:862	∼8 MPa	857:862	The mechanical properties of the films can be tuned from elastic (∼8 MPa) chitosan films to stiff (∼285 MPa) bionanocomposite films with 50% filler.
31960003	6	48	theme	elastic	848:854	arg1	films					874:878	elastic (∼8 MPa) chitosan films	848:878	elastic (∼8 MPa) chitosan films	848:878	The mechanical properties of the films can be tuned from elastic (∼8 MPa) chitosan films to stiff (∼285 MPa) bionanocomposite films with 50% filler.
31960003	2	49	theme	oxide-iron	414:423	arg1	rGO-Fe3-xO4					432:442	rGO-Fe3-xO4	432:442	rGO-Fe3-xO4	432:442	Here, the development of flexible and multifunctional chitosan-based bionanocomposites with a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler is described.
31960003	2	49	theme	oxide-iron	414:423	arg1	oxide					425:429	reduced graphene oxide-iron oxide	397:429	a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler	389:450	Here, the development of flexible and multifunctional chitosan-based bionanocomposites with a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler is described.
31960003	6	50	theme	bionanocomposite	900:915	arg1	films					917:921	stiff (∼285 MPa) bionanocomposite films	883:921	stiff (∼285 MPa) bionanocomposite films with 50% filler	883:937	The mechanical properties of the films can be tuned from elastic (∼8 MPa) chitosan films to stiff (∼285 MPa) bionanocomposite films with 50% filler.
31960003	2	51	theme	graphene	405:412	arg1	rGO-Fe3-xO4					432:442	rGO-Fe3-xO4	432:442	rGO-Fe3-xO4	432:442	Here, the development of flexible and multifunctional chitosan-based bionanocomposites with a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler is described.
31960003	2	51	theme	graphene	405:412	arg1	oxide					425:429	reduced graphene oxide-iron oxide	397:429	a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler	389:450	Here, the development of flexible and multifunctional chitosan-based bionanocomposites with a mixed reduced graphene oxide-iron oxide (rGO-Fe3-xO4) filler is described.
31960003	7	52	theme	temperature	981:991	arg1	increase					993:1000	a temperature increase	979:1000	a temperature increase of 40 °C in 45 s	979:1017	The magnetic hyperthermia tests showed a temperature increase of 40 °C in 45 s for the 50% rGO-Fe3-xO4 film.
31960003	1	53	theme	biomedical	272:281	arg1	applications					283:294	several emerging biomedical applications	255:294	several emerging biomedical applications	255:294	Sustainably made, flexible and biocompatible composites, having environmentally friendly compositions and multifunctional capabilities, are promising materials for several emerging biomedical applications.
31960003	1	54	theme	made	103:106	arg1	composites					136:145	Sustainably made, flexible and biocompatible composites	91:145	Sustainably made, flexible and biocompatible composites	91:145	Sustainably made, flexible and biocompatible composites, having environmentally friendly compositions and multifunctional capabilities, are promising materials for several emerging biomedical applications.
31960003	1	54	theme	made	103:106	arg1	materials					241:249	promising materials	231:249	promising materials for several emerging biomedical applications	231:294	Sustainably made, flexible and biocompatible composites, having environmentally friendly compositions and multifunctional capabilities, are promising materials for several emerging biomedical applications.
31960003	8	55	theme	materials	1215:1223	arg1	potential					1196:1204	the potential	1192:1204	the potential of these materials for biomedical applications, such as hyperthermia treatments	1192:1284	Furthermore, the composites have no cytotoxicity to the nontumorigenic (HaCat) cell line, which confirms their biocompatibility and highlights the potential of these materials for biomedical applications, such as hyperthermia treatments.
31960003	7	56	theme	%	1029:1029	arg1	film					1043:1046	the 50% rGO-Fe3-xO4 film	1023:1046	the 50% rGO-Fe3-xO4 film	1023:1046	The magnetic hyperthermia tests showed a temperature increase of 40 °C in 45 s for the 50% rGO-Fe3-xO4 film.
31960003	1	57	theme	environmentally	155:169	arg1	compositions					180:191	environmentally friendly compositions	155:191	environmentally friendly compositions	155:191	Sustainably made, flexible and biocompatible composites, having environmentally friendly compositions and multifunctional capabilities, are promising materials for several emerging biomedical applications.
31960003	3	58	theme	Fe3-xO4	547:553	arg1	nanoparticles					555:567	Fe3-xO4 nanoparticles	547:567	Fe3-xO4 nanoparticles	547:567	The filler is prepared by one-pot synthesis, ensuring good dispersibility of the Fe3-xO4 nanoparticles and rGO within the chitosan matrix during solvent casting.
31960003	6	59	theme	%	930:930	arg1	filler					932:937	50% filler	928:937	50% filler	928:937	The mechanical properties of the films can be tuned from elastic (∼8 MPa) chitosan films to stiff (∼285 MPa) bionanocomposite films with 50% filler.
31960003	7	60	theme	rGO-Fe3-xO4	1031:1041	arg1	film					1043:1046	the 50% rGO-Fe3-xO4 film	1023:1046	the 50% rGO-Fe3-xO4 film	1023:1046	The magnetic hyperthermia tests showed a temperature increase of 40 °C in 45 s for the 50% rGO-Fe3-xO4 film.
31960003	1	61	theme	friendly	171:178	arg1	compositions					180:191	environmentally friendly compositions	155:191	environmentally friendly compositions	155:191	Sustainably made, flexible and biocompatible composites, having environmentally friendly compositions and multifunctional capabilities, are promising materials for several emerging biomedical applications.
31960003	3	62	theme	nanoparticles	555:567	arg1	dispersibility					525:538	good dispersibility	520:538	good dispersibility of the Fe3-xO4 nanoparticles and rGO within the chitosan matrix during solvent casting	520:625	The filler is prepared by one-pot synthesis, ensuring good dispersibility of the Fe3-xO4 nanoparticles and rGO within the chitosan matrix during solvent casting.
31960003	8	63	theme	hyperthermia	1262:1273	arg1	treatments					1275:1284	hyperthermia treatments	1262:1284	hyperthermia treatments	1262:1284	Furthermore, the composites have no cytotoxicity to the nontumorigenic (HaCat) cell line, which confirms their biocompatibility and highlights the potential of these materials for biomedical applications, such as hyperthermia treatments.
31960003	6	64	from	films	874:878	arg1	properties					806:815	The mechanical properties	791:815	The mechanical properties of the films	791:828	The mechanical properties of the films can be tuned from elastic (∼8 MPa) chitosan films to stiff (∼285 MPa) bionanocomposite films with 50% filler.
31960003	6	64	from	films	874:878	arg1	tuned					837:841	tuned	837:841	tuned	837:841	The mechanical properties of the films can be tuned from elastic (∼8 MPa) chitosan films to stiff (∼285 MPa) bionanocomposite films with 50% filler.
31960003	3	65	theme	rGO	573:575	arg1	dispersibility					525:538	good dispersibility	520:538	good dispersibility of the Fe3-xO4 nanoparticles and rGO within the chitosan matrix during solvent casting	520:625	The filler is prepared by one-pot synthesis, ensuring good dispersibility of the Fe3-xO4 nanoparticles and rGO within the chitosan matrix during solvent casting.
34255124	3	0	theme	cps4H	683:687	arg1	level					674:678	the expression level	659:678	the expression level of cps4H	659:687	The transcriptomic data revealed that the exposure of L. plantarum VAL6 at pH 3 increased the expression level of cps4H but decreased the expression levels of pgmB1 and cps4E.
34255124	6	1	theme	4	1154:1154	arg1	%					1155:1155	%	1155:1155	%	1155:1155	In the case of NaCl stress, glmU, pgmB1, cps4J, and cps4H were downregulated in exposure to NaCl at 7 and 10% concentrations while cps4E and cps4F were upregulated at 1 h of 10%-NaCl treatment and at 5 h of 4%-NaCl treatment.
34255124	1	2	theme	lactic	194:199	arg1	LAB					216:218	LAB	216:218	LAB	216:218	Environmental factors can alter exopolysaccharide biosynthesis in lactic acid bacteria (LAB).
34255124	1	2	theme	lactic	194:199	arg1	bacteria					206:213	lactic acid bacteria	194:213	lactic acid bacteria (LAB)	194:219	Environmental factors can alter exopolysaccharide biosynthesis in lactic acid bacteria (LAB).
34255124	3	3	theme	expression	663:672	arg1	level					674:678	the expression level	659:678	the expression level of cps4H	659:687	The transcriptomic data revealed that the exposure of L. plantarum VAL6 at pH 3 increased the expression level of cps4H but decreased the expression levels of pgmB1 and cps4E.
34255124	6	4	theme	-NaCl	1124:1128	arg1	treatment					1130:1138	10%-NaCl treatment	1121:1138	10%-NaCl treatment	1121:1138	In the case of NaCl stress, glmU, pgmB1, cps4J, and cps4H were downregulated in exposure to NaCl at 7 and 10% concentrations while cps4E and cps4F were upregulated at 1 h of 10%-NaCl treatment and at 5 h of 4%-NaCl treatment.
34255124	2	5	from	pgmB1	353:357	arg1	VAL6					424:427	Lactiplantibacillus plantarum VAL6	394:427	Lactiplantibacillus plantarum VAL6	394:427	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	1	6	theme	acid	201:204	arg1	LAB					216:218	LAB	216:218	LAB	216:218	Environmental factors can alter exopolysaccharide biosynthesis in lactic acid bacteria (LAB).
34255124	1	6	theme	acid	201:204	arg1	bacteria					206:213	lactic acid bacteria	194:213	lactic acid bacteria (LAB)	194:219	Environmental factors can alter exopolysaccharide biosynthesis in lactic acid bacteria (LAB).
34255124	2	7	from	glmU	347:350	arg1	VAL6					424:427	Lactiplantibacillus plantarum VAL6	394:427	Lactiplantibacillus plantarum VAL6	394:427	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	8	8	theme	cps4E	1311:1315	arg1	expression					1297:1306	expression	1297:1306	expression of cps4E and cps4F under environmental challenges	1297:1356	In addition, simultaneous changes in expression of cps4E and cps4F under environmental challenges may elicit the possibility of an association between the two genes.
34255124	2	9	theme	exopolysaccharide	311:327	arg1	synthesis					329:337	exopolysaccharide synthesis	311:337	exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture	311:554	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	0	10	theme	environmental	104:116	arg1	stresses					118:125	environmental stresses	104:125	environmental stresses	104:125	Expression of genes involved in exopolysaccharide synthesis in Lactiplantibacillus plantarum VAL6 under environmental stresses.
34255124	8	11	theme	association	1391:1401	arg1	possibility					1373:1383	the possibility	1369:1383	the possibility of an association between the two genes	1369:1423	In addition, simultaneous changes in expression of cps4E and cps4F under environmental challenges may elicit the possibility of an association between the two genes.
34255124	2	12	from	cps4E	360:364	arg1	VAL6					424:427	Lactiplantibacillus plantarum VAL6	394:427	Lactiplantibacillus plantarum VAL6	394:427	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	0	13	from	synthesis	50:58	arg1	VAL6					93:96	Lactiplantibacillus plantarum VAL6	63:96	Lactiplantibacillus plantarum VAL6	63:96	Expression of genes involved in exopolysaccharide synthesis in Lactiplantibacillus plantarum VAL6 under environmental stresses.
34255124	7	14	theme	CO2	1185:1187	arg1	culture					1205:1211	CO2 intensification culture	1185:1211	CO2 intensification culture	1185:1211	Remarkably, CO2 intensification culture stimulated the expression of all tested genes.
34255124	0	15	theme	exopolysaccharide	32:48	arg1	synthesis					50:58	exopolysaccharide synthesis	32:58	exopolysaccharide synthesis in Lactiplantibacillus plantarum VAL6 under environmental stresses	32:125	Expression of genes involved in exopolysaccharide synthesis in Lactiplantibacillus plantarum VAL6 under environmental stresses.
34255124	2	16	from	cps4F	367:371	arg1	VAL6					424:427	Lactiplantibacillus plantarum VAL6	394:427	Lactiplantibacillus plantarum VAL6	394:427	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	2	17	theme	genes	293:297	arg1	expression					279:288	the mRNA expression	270:288	the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture	270:554	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	9	18	from	changes	1509:1515	arg1	yield					1524:1528	yield	1524:1528	yield	1524:1528	These findings indicated that the expression level of eps genes is responsible for changes in the yield and monosaccharide composition of exopolysaccharides under environmental stresses.
34255124	9	18	from	changes	1509:1515	arg1	composition					1549:1559	monosaccharide composition	1534:1559	monosaccharide composition	1534:1559	These findings indicated that the expression level of eps genes is responsible for changes in the yield and monosaccharide composition of exopolysaccharides under environmental stresses.
34255124	7	19	theme	intensification	1189:1203	arg1	culture					1205:1211	CO2 intensification culture	1185:1211	CO2 intensification culture	1185:1211	Remarkably, CO2 intensification culture stimulated the expression of all tested genes.
34255124	2	20	dep	temperature	466:476	arg1	culture					548:554	intensification culture	532:554	intensification culture	532:554	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	3	21	from	pH	644:645	arg1	exposure					611:618	the exposure	607:618	the exposure of L. plantarum VAL6 at pH 3	607:647	The transcriptomic data revealed that the exposure of L. plantarum VAL6 at pH 3 increased the expression level of cps4H but decreased the expression levels of pgmB1 and cps4E.
34255124	3	22	theme	expression	707:716	arg1	levels					718:723	the expression levels	703:723	the expression levels of pgmB1 and cps4E	703:742	The transcriptomic data revealed that the exposure of L. plantarum VAL6 at pH 3 increased the expression level of cps4H but decreased the expression levels of pgmB1 and cps4E.
34255124	9	23	theme	expression	1460:1469	arg1	responsible					1493:1503	responsible	1493:1503	responsible	1493:1503	These findings indicated that the expression level of eps genes is responsible for changes in the yield and monosaccharide composition of exopolysaccharides under environmental stresses.
34255124	9	23	theme	expression	1460:1469	arg1	level					1471:1475	the expression level	1456:1475	the expression level of eps genes	1456:1488	These findings indicated that the expression level of eps genes is responsible for changes in the yield and monosaccharide composition of exopolysaccharides under environmental stresses.
34255124	9	24	dep	yield	1524:1528	arg1	the					1520:1522	the	1520:1522	the	1520:1522	These findings indicated that the expression level of eps genes is responsible for changes in the yield and monosaccharide composition of exopolysaccharides under environmental stresses.
34255124	3	25	theme	pgmB1	728:732	arg1	levels					718:723	the expression levels	703:723	the expression levels of pgmB1 and cps4E	703:742	The transcriptomic data revealed that the exposure of L. plantarum VAL6 at pH 3 increased the expression level of cps4H but decreased the expression levels of pgmB1 and cps4E.
34255124	6	26	theme	%	1123:1123	arg1	treatment					1130:1138	10%-NaCl treatment	1121:1138	10%-NaCl treatment	1121:1138	In the case of NaCl stress, glmU, pgmB1, cps4J, and cps4H were downregulated in exposure to NaCl at 7 and 10% concentrations while cps4E and cps4F were upregulated at 1 h of 10%-NaCl treatment and at 5 h of 4%-NaCl treatment.
34255124	2	27	from	cps4J	374:378	arg1	VAL6					424:427	Lactiplantibacillus plantarum VAL6	394:427	Lactiplantibacillus plantarum VAL6	394:427	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	5	28	theme	cps4J	892:896	arg1	levels					864:869	the expression levels	849:869	the expression levels of cps4F, cps4E, and cps4J	849:896	Similarly, the expression levels of cps4F, cps4E, and cps4J increased sharply under stresses at 42 or 47 °C.
34255124	2	29	theme	intensification	532:546	arg1	culture					548:554	intensification culture	532:554	intensification culture	532:554	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	9	30	theme	eps	1480:1482	arg1	genes					1484:1488	eps genes	1480:1488	eps genes	1480:1488	These findings indicated that the expression level of eps genes is responsible for changes in the yield and monosaccharide composition of exopolysaccharides under environmental stresses.
34255124	7	31	theme	tested	1246:1251	arg1	genes					1253:1257	all tested genes	1242:1257	all tested genes	1242:1257	Remarkably, CO2 intensification culture stimulated the expression of all tested genes.
34255124	6	32	dep	concentrations	1057:1070	arg1	%					1055:1055	%	1055:1055	%	1055:1055	In the case of NaCl stress, glmU, pgmB1, cps4J, and cps4H were downregulated in exposure to NaCl at 7 and 10% concentrations while cps4E and cps4F were upregulated at 1 h of 10%-NaCl treatment and at 5 h of 4%-NaCl treatment.
34255124	2	33	theme	different	435:443	arg1	pH					479:480	pH	479:480	pH	479:480	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	2	33	theme	different	435:443	arg1	chloride					490:497	sodium chloride	483:497	sodium chloride (NaCl)	483:504	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	2	33	theme	different	435:443	arg1	temperature					466:476	temperature	466:476	temperature	466:476	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	2	33	theme	different	435:443	arg1	conditions					445:454	different conditions	435:454	different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture	435:554	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	2	33	theme	different	435:443	arg1	dioxide					518:524	carbon dioxide	511:524	carbon dioxide (CO2)	511:530	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	3	34	theme	VAL6	636:639	arg1	exposure					611:618	the exposure	607:618	the exposure of L. plantarum VAL6 at pH 3	607:647	The transcriptomic data revealed that the exposure of L. plantarum VAL6 at pH 3 increased the expression level of cps4H but decreased the expression levels of pgmB1 and cps4E.
34255124	1	35	theme	Environmental	128:140	arg1	factors					142:148	Environmental factors	128:148	Environmental factors	128:148	Environmental factors can alter exopolysaccharide biosynthesis in lactic acid bacteria (LAB).
34255124	8	36	from	changes	1286:1292	arg1	expression					1297:1306	expression	1297:1306	expression of cps4E and cps4F under environmental challenges	1297:1356	In addition, simultaneous changes in expression of cps4E and cps4F under environmental challenges may elicit the possibility of an association between the two genes.
34255124	6	37	dep	%	1055:1055	arg1	10					1053:1054	10	1053:1054	10	1053:1054	In the case of NaCl stress, glmU, pgmB1, cps4J, and cps4H were downregulated in exposure to NaCl at 7 and 10% concentrations while cps4E and cps4F were upregulated at 1 h of 10%-NaCl treatment and at 5 h of 4%-NaCl treatment.
34255124	6	37	dep	%	1055:1055	arg1	7					1047:1047	7	1047:1047	7	1047:1047	In the case of NaCl stress, glmU, pgmB1, cps4J, and cps4H were downregulated in exposure to NaCl at 7 and 10% concentrations while cps4E and cps4F were upregulated at 1 h of 10%-NaCl treatment and at 5 h of 4%-NaCl treatment.
34255124	2	38	theme	sodium	483:488	arg1	NaCl					500:503	NaCl	500:503	NaCl	500:503	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	2	38	theme	sodium	483:488	arg1	chloride					490:497	sodium chloride	483:497	sodium chloride (NaCl)	483:504	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	9	39	theme	environmental	1589:1601	arg1	stresses					1603:1610	environmental stresses	1589:1610	environmental stresses	1589:1610	These findings indicated that the expression level of eps genes is responsible for changes in the yield and monosaccharide composition of exopolysaccharides under environmental stresses.
34255124	6	40	theme	10	1121:1122	arg1	%					1123:1123	%	1123:1123	%	1123:1123	In the case of NaCl stress, glmU, pgmB1, cps4J, and cps4H were downregulated in exposure to NaCl at 7 and 10% concentrations while cps4E and cps4F were upregulated at 1 h of 10%-NaCl treatment and at 5 h of 4%-NaCl treatment.
34255124	2	41	theme	plantarum	414:422	arg1	VAL6					424:427	Lactiplantibacillus plantarum VAL6	394:427	Lactiplantibacillus plantarum VAL6	394:427	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	6	42	theme	treatment	1130:1138	arg1	1 h					1114:1116	1 h	1114:1116	1 h of 10%-NaCl treatment	1114:1138	In the case of NaCl stress, glmU, pgmB1, cps4J, and cps4H were downregulated in exposure to NaCl at 7 and 10% concentrations while cps4E and cps4F were upregulated at 1 h of 10%-NaCl treatment and at 5 h of 4%-NaCl treatment.
34255124	3	43	theme	L.	623:624	arg1	VAL6					636:639	L. plantarum VAL6	623:639	L. plantarum VAL6	623:639	The transcriptomic data revealed that the exposure of L. plantarum VAL6 at pH 3 increased the expression level of cps4H but decreased the expression levels of pgmB1 and cps4E.
34255124	7	44	theme	genes	1253:1257	arg1	expression					1228:1237	the expression	1224:1237	the expression of all tested genes	1224:1257	Remarkably, CO2 intensification culture stimulated the expression of all tested genes.
34255124	2	45	theme	Lactiplantibacillus	394:412	arg1	VAL6					424:427	Lactiplantibacillus plantarum VAL6	394:427	Lactiplantibacillus plantarum VAL6	394:427	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	3	46	theme	plantarum	626:634	arg1	VAL6					636:639	L. plantarum VAL6	623:639	L. plantarum VAL6	623:639	The transcriptomic data revealed that the exposure of L. plantarum VAL6 at pH 3 increased the expression level of cps4H but decreased the expression levels of pgmB1 and cps4E.
34255124	2	47	theme	potential	246:254	arg1	relationship					256:267	this potential relationship	241:267	this potential relationship	241:267	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	5	48	theme	cps4F	874:878	arg1	levels					864:869	the expression levels	849:869	the expression levels of cps4F, cps4E, and cps4J	849:896	Similarly, the expression levels of cps4F, cps4E, and cps4J increased sharply under stresses at 42 or 47 °C.
34255124	3	49	theme	cps4E	738:742	arg1	levels					718:723	the expression levels	703:723	the expression levels of pgmB1 and cps4E	703:742	The transcriptomic data revealed that the exposure of L. plantarum VAL6 at pH 3 increased the expression level of cps4H but decreased the expression levels of pgmB1 and cps4E.
34255124	6	50	theme	treatment	1162:1170	arg1	5 h					1147:1149	5 h	1147:1149	5 h of 4%-NaCl treatment	1147:1170	In the case of NaCl stress, glmU, pgmB1, cps4J, and cps4H were downregulated in exposure to NaCl at 7 and 10% concentrations while cps4E and cps4F were upregulated at 1 h of 10%-NaCl treatment and at 5 h of 4%-NaCl treatment.
34255124	8	51	theme	environmental	1333:1345	arg1	challenges					1347:1356	environmental challenges	1333:1356	environmental challenges	1333:1356	In addition, simultaneous changes in expression of cps4E and cps4F under environmental challenges may elicit the possibility of an association between the two genes.
34255124	3	52	theme	transcriptomic	573:586	arg1	data					588:591	The transcriptomic data	569:591	The transcriptomic data	569:591	The transcriptomic data revealed that the exposure of L. plantarum VAL6 at pH 3 increased the expression level of cps4H but decreased the expression levels of pgmB1 and cps4E.
34255124	8	53	theme	cps4F	1321:1325	arg1	expression					1297:1306	expression	1297:1306	expression of cps4E and cps4F under environmental challenges	1297:1356	In addition, simultaneous changes in expression of cps4E and cps4F under environmental challenges may elicit the possibility of an association between the two genes.
34255124	9	54	theme	exopolysaccharides	1564:1581	arg1	yield					1524:1528	yield	1524:1528	yield	1524:1528	These findings indicated that the expression level of eps genes is responsible for changes in the yield and monosaccharide composition of exopolysaccharides under environmental stresses.
34255124	9	54	theme	exopolysaccharides	1564:1581	arg1	composition					1549:1559	monosaccharide composition	1534:1559	monosaccharide composition	1534:1559	These findings indicated that the expression level of eps genes is responsible for changes in the yield and monosaccharide composition of exopolysaccharides under environmental stresses.
34255124	6	55	theme	-NaCl	1156:1160	arg1	treatment					1162:1170	4%-NaCl treatment	1154:1170	4%-NaCl treatment	1154:1170	In the case of NaCl stress, glmU, pgmB1, cps4J, and cps4H were downregulated in exposure to NaCl at 7 and 10% concentrations while cps4E and cps4F were upregulated at 1 h of 10%-NaCl treatment and at 5 h of 4%-NaCl treatment.
34255124	9	56	theme	genes	1484:1488	arg1	responsible					1493:1503	responsible	1493:1503	responsible	1493:1503	These findings indicated that the expression level of eps genes is responsible for changes in the yield and monosaccharide composition of exopolysaccharides under environmental stresses.
34255124	9	56	theme	genes	1484:1488	arg1	level					1471:1475	the expression level	1456:1475	the expression level of eps genes	1456:1488	These findings indicated that the expression level of eps genes is responsible for changes in the yield and monosaccharide composition of exopolysaccharides under environmental stresses.
34255124	2	57	from	cps4H	385:389	arg1	VAL6					424:427	Lactiplantibacillus plantarum VAL6	394:427	Lactiplantibacillus plantarum VAL6	394:427	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	5	58	theme	cps4E	881:885	arg1	levels					864:869	the expression levels	849:869	the expression levels of cps4F, cps4E, and cps4J	849:896	Similarly, the expression levels of cps4F, cps4E, and cps4J increased sharply under stresses at 42 or 47 °C.
34255124	6	59	theme	NaCl	962:965	arg1	stress					967:972	NaCl stress	962:972	NaCl stress	962:972	In the case of NaCl stress, glmU, pgmB1, cps4J, and cps4H were downregulated in exposure to NaCl at 7 and 10% concentrations while cps4E and cps4F were upregulated at 1 h of 10%-NaCl treatment and at 5 h of 4%-NaCl treatment.
34255124	6	60	theme	%	1155:1155	arg1	treatment					1162:1170	4%-NaCl treatment	1154:1170	4%-NaCl treatment	1154:1170	In the case of NaCl stress, glmU, pgmB1, cps4J, and cps4H were downregulated in exposure to NaCl at 7 and 10% concentrations while cps4E and cps4F were upregulated at 1 h of 10%-NaCl treatment and at 5 h of 4%-NaCl treatment.
34255124	8	61	theme	simultaneous	1273:1284	arg1	changes					1286:1292	simultaneous changes	1273:1292	simultaneous changes in expression of cps4E and cps4F under environmental challenges	1273:1356	In addition, simultaneous changes in expression of cps4E and cps4F under environmental challenges may elicit the possibility of an association between the two genes.
34255124	1	62	theme	exopolysaccharide	160:176	arg1	biosynthesis					178:189	exopolysaccharide biosynthesis	160:189	exopolysaccharide biosynthesis	160:189	Environmental factors can alter exopolysaccharide biosynthesis in lactic acid bacteria (LAB).
34255124	0	63	theme	plantarum	83:91	arg1	VAL6					93:96	Lactiplantibacillus plantarum VAL6	63:96	Lactiplantibacillus plantarum VAL6	63:96	Expression of genes involved in exopolysaccharide synthesis in Lactiplantibacillus plantarum VAL6 under environmental stresses.
34255124	6	64	theme	stress	967:972	arg1	case					954:957	the case	950:957	the case of NaCl stress	950:972	In the case of NaCl stress, glmU, pgmB1, cps4J, and cps4H were downregulated in exposure to NaCl at 7 and 10% concentrations while cps4E and cps4F were upregulated at 1 h of 10%-NaCl treatment and at 5 h of 4%-NaCl treatment.
34255124	2	65	theme	mRNA	274:277	arg1	expression					279:288	the mRNA expression	270:288	the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture	270:554	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	9	66	theme	monosaccharide	1534:1547	arg1	composition					1549:1559	monosaccharide composition	1534:1559	monosaccharide composition	1534:1559	These findings indicated that the expression level of eps genes is responsible for changes in the yield and monosaccharide composition of exopolysaccharides under environmental stresses.
34255124	0	67	theme	Lactiplantibacillus	63:81	arg1	VAL6					93:96	Lactiplantibacillus plantarum VAL6	63:96	Lactiplantibacillus plantarum VAL6	63:96	Expression of genes involved in exopolysaccharide synthesis in Lactiplantibacillus plantarum VAL6 under environmental stresses.
34255124	0	68	theme	genes	14:18	arg1	Expression					0:9	Expression	0:9	Expression of genes	0:18	Expression of genes involved in exopolysaccharide synthesis in Lactiplantibacillus plantarum VAL6 under environmental stresses.
34255124	5	69	theme	expression	853:862	arg1	levels					864:869	the expression levels	849:869	the expression levels of cps4F, cps4E, and cps4J	849:896	Similarly, the expression levels of cps4F, cps4E, and cps4J increased sharply under stresses at 42 or 47 °C.
34255124	2	70	theme	carbon	511:516	arg1	CO2					527:529	CO2	527:529	CO2	527:529	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34255124	2	70	theme	carbon	511:516	arg1	dioxide					518:524	carbon dioxide	511:524	carbon dioxide (CO2)	511:530	To further clarify this potential relationship, the mRNA expression of genes involved in exopolysaccharide synthesis such as glmU, pgmB1, cps4E, cps4F, cps4J, and cps4H in Lactiplantibacillus plantarum VAL6 under different conditions including temperature, pH, sodium chloride (NaCl), and carbon dioxide (CO2) intensification culture was studied.
34082941	8	0	theme	no-lithium	1153:1162	arg1	fibres					1164:1169	no-lithium fibres	1153:1169	no-lithium fibres	1153:1169	Furthermore, the chondrogenic behavior on HA constructs with fibres containing lithium is more marked than in hydrogels with no-lithium fibres.
34082941	4	1	theme	therapeutic	686:696	arg1	lithium-ions					698:709	therapeutic lithium-ions	686:709	therapeutic lithium-ions	686:709	The glass fibres are a reinforcement filler and a platform for the delivery of therapeutic lithium-ions.
34082941	2	2	theme	extracellular	354:366	arg1	matrix					368:373	cartilage extracellular matrix	344:373	cartilage extracellular matrix	344:373	Hydrogels are candidates to repair lesions as they have similar properties to cartilage extracellular matrix but they are unable to meet the mechanical and biological requirements for a successful outcome.
34082941	5	3	theme	composites	739:748	arg1	modulus					724:730	The elastic modulus	712:730	The elastic modulus of the composites	712:748	The elastic modulus of the composites is more than three times higher than in HA hydrogels.
34082941	5	3	theme	composites	739:748	arg1	times					769:773	more than three times	753:773	more than three times higher than in HA hydrogels	753:801	The elastic modulus of the composites is more than three times higher than in HA hydrogels.
34082941	1	4	theme	weight-bearing	156:169	arg1	joints					171:176	weight-bearing joints	156:176	weight-bearing joints	156:176	The repair of articular cartilage lesions in weight-bearing joints remains as a significant challenge due to the low regenerative capacity of this tissue.
34082941	7	5	theme	ATDC5	874:878	arg1	cells					893:897	ATDC5 chondrogenic cells	874:897	ATDC5 chondrogenic cells seeded on the composites	874:922	ATDC5 chondrogenic cells seeded on the composites are viable and more proliferation occurs on the hydrogels containing fibres than in HA hydrogels alone.
34082941	8	6	from	marked	1123:1128	arg1	hydrogels					1138:1146	hydrogels	1138:1146	hydrogels with no-lithium fibres	1138:1169	Furthermore, the chondrogenic behavior on HA constructs with fibres containing lithium is more marked than in hydrogels with no-lithium fibres.
34082941	8	7	theme	chondrogenic	1045:1056	arg1	marked					1123:1128	marked	1123:1128	marked	1123:1128	Furthermore, the chondrogenic behavior on HA constructs with fibres containing lithium is more marked than in hydrogels with no-lithium fibres.
34082941	8	7	theme	chondrogenic	1045:1056	arg1	behavior					1058:1065	the chondrogenic behavior	1041:1065	the chondrogenic behavior on HA constructs with fibres containing lithium	1041:1113	Furthermore, the chondrogenic behavior on HA constructs with fibres containing lithium is more marked than in hydrogels with no-lithium fibres.
34082941	8	8	contain	containing	1096:1105	arg2	lithium					1107:1113	lithium	1107:1113	lithium	1107:1113	Furthermore, the chondrogenic behavior on HA constructs with fibres containing lithium is more marked than in hydrogels with no-lithium fibres.
34082941	8	8	contain	containing	1096:1105	arg1	fibres					1089:1094	fibres	1089:1094	fibres containing lithium	1089:1113	Furthermore, the chondrogenic behavior on HA constructs with fibres containing lithium is more marked than in hydrogels with no-lithium fibres.
34082941	3	9	theme	13-93-lithium	527:539	arg1	micro-					557:562	13-93-lithium bioactive glass micro-	527:562	13-93-lithium bioactive glass micro-	527:562	Here, we reinforce hyaluronic acid (HA) hydrogels with 13-93-lithium bioactive glass micro- and nanofibres produced by laser spinning.
34082941	2	10	theme	repair	294:299	arg1	lesions					301:307	repair lesions	294:307	repair lesions	294:307	Hydrogels are candidates to repair lesions as they have similar properties to cartilage extracellular matrix but they are unable to meet the mechanical and biological requirements for a successful outcome.
34082941	1	11	theme	tissue	258:263	arg1	capacity					241:248	the low regenerative capacity	220:248	the low regenerative capacity of this tissue	220:263	The repair of articular cartilage lesions in weight-bearing joints remains as a significant challenge due to the low regenerative capacity of this tissue.
34082941	3	12	theme	bioactive	541:549	arg1	micro-					557:562	13-93-lithium bioactive glass micro-	527:562	13-93-lithium bioactive glass micro-	527:562	Here, we reinforce hyaluronic acid (HA) hydrogels with 13-93-lithium bioactive glass micro- and nanofibres produced by laser spinning.
34082941	8	13	with	hydrogels	1138:1146	arg1	fibres					1164:1169	no-lithium fibres	1153:1169	no-lithium fibres	1153:1169	Furthermore, the chondrogenic behavior on HA constructs with fibres containing lithium is more marked than in hydrogels with no-lithium fibres.
34082941	3	14	theme	acid	502:505	arg1	hydrogels					512:520	hyaluronic acid (HA) hydrogels	491:520	hyaluronic acid (HA) hydrogels with 13-93-lithium bioactive glass micro- and nanofibres produced by laser spinning	491:604	Here, we reinforce hyaluronic acid (HA) hydrogels with 13-93-lithium bioactive glass micro- and nanofibres produced by laser spinning.
34082941	2	15	theme	successful	452:461	arg1	outcome					463:469	a successful outcome	450:469	a successful outcome	450:469	Hydrogels are candidates to repair lesions as they have similar properties to cartilage extracellular matrix but they are unable to meet the mechanical and biological requirements for a successful outcome.
34082941	3	16	with	hydrogels	512:520	arg1	micro-					557:562	13-93-lithium bioactive glass micro-	527:562	13-93-lithium bioactive glass micro-	527:562	Here, we reinforce hyaluronic acid (HA) hydrogels with 13-93-lithium bioactive glass micro- and nanofibres produced by laser spinning.
34082941	3	16	with	hydrogels	512:520	arg1	nanofibres					568:577	nanofibres	568:577	nanofibres produced by laser spinning	568:604	Here, we reinforce hyaluronic acid (HA) hydrogels with 13-93-lithium bioactive glass micro- and nanofibres produced by laser spinning.
34082941	8	17	from	hydrogels	1138:1146	arg1	marked					1123:1128	marked	1123:1128	marked	1123:1128	Furthermore, the chondrogenic behavior on HA constructs with fibres containing lithium is more marked than in hydrogels with no-lithium fibres.
34082941	8	17	from	hydrogels	1138:1146	arg1	behavior					1058:1065	the chondrogenic behavior	1041:1065	the chondrogenic behavior on HA constructs with fibres containing lithium	1041:1113	Furthermore, the chondrogenic behavior on HA constructs with fibres containing lithium is more marked than in hydrogels with no-lithium fibres.
34082941	7	18	from	fibres	993:998	arg1	hydrogels					1011:1019	HA hydrogels	1008:1019	HA hydrogels alone	1008:1025	ATDC5 chondrogenic cells seeded on the composites are viable and more proliferation occurs on the hydrogels containing fibres than in HA hydrogels alone.
34082941	5	19	theme	HA	790:791	arg1	hydrogels					793:801	HA hydrogels	790:801	HA hydrogels	790:801	The elastic modulus of the composites is more than three times higher than in HA hydrogels.
34082941	0	20	dep	nanofibres	80:89	arg1	doped					91:95	doped	91:95	nanofibres doped with lithium	80:108	Hyaluronic acid hydrogels reinforced with laser spun bioactive glass micro- and nanofibres doped with lithium.
34082941	0	21	theme	Hyaluronic	0:9	arg1	acid					11:14	Hyaluronic acid	0:14	Hyaluronic acid	0:14	Hyaluronic acid hydrogels reinforced with laser spun bioactive glass micro- and nanofibres doped with lithium.
34082941	8	22	theme	HA	1070:1071	arg1	constructs					1073:1082	HA constructs	1070:1082	HA constructs with fibres containing lithium	1070:1113	Furthermore, the chondrogenic behavior on HA constructs with fibres containing lithium is more marked than in hydrogels with no-lithium fibres.
34082941	8	23	with	constructs	1073:1082	arg1	fibres					1089:1094	fibres	1089:1094	fibres containing lithium	1089:1113	Furthermore, the chondrogenic behavior on HA constructs with fibres containing lithium is more marked than in hydrogels with no-lithium fibres.
34082941	1	24	from	repair	115:120	arg1	joints					171:176	weight-bearing joints	156:176	weight-bearing joints	156:176	The repair of articular cartilage lesions in weight-bearing joints remains as a significant challenge due to the low regenerative capacity of this tissue.
34082941	3	25	theme	glass	551:555	arg1	micro-					557:562	13-93-lithium bioactive glass micro-	527:562	13-93-lithium bioactive glass micro-	527:562	Here, we reinforce hyaluronic acid (HA) hydrogels with 13-93-lithium bioactive glass micro- and nanofibres produced by laser spinning.
34082941	1	26	theme	significant	191:201	arg1	challenge					203:211	a significant challenge	189:211	a significant challenge due to the low regenerative capacity of this tissue	189:263	The repair of articular cartilage lesions in weight-bearing joints remains as a significant challenge due to the low regenerative capacity of this tissue.
34082941	2	27	theme	biological	422:431	arg1	requirements					433:444	the mechanical and biological requirements	403:444	the mechanical and biological requirements for a successful outcome	403:469	Hydrogels are candidates to repair lesions as they have similar properties to cartilage extracellular matrix but they are unable to meet the mechanical and biological requirements for a successful outcome.
34082941	2	28	theme	cartilage	344:352	arg1	matrix					368:373	cartilage extracellular matrix	344:373	cartilage extracellular matrix	344:373	Hydrogels are candidates to repair lesions as they have similar properties to cartilage extracellular matrix but they are unable to meet the mechanical and biological requirements for a successful outcome.
34082941	2	29	theme	mechanical	407:416	arg1	requirements					433:444	the mechanical and biological requirements	403:444	the mechanical and biological requirements for a successful outcome	403:469	Hydrogels are candidates to repair lesions as they have similar properties to cartilage extracellular matrix but they are unable to meet the mechanical and biological requirements for a successful outcome.
34082941	0	30	theme	laser	42:46	arg1	micro-					69:74	laser spun bioactive glass micro-	42:74	laser spun bioactive glass micro-	42:74	Hyaluronic acid hydrogels reinforced with laser spun bioactive glass micro- and nanofibres doped with lithium.
34082941	4	31	theme	lithium-ions	698:709	arg1	delivery					674:681	the delivery	670:681	the delivery of therapeutic lithium-ions	670:709	The glass fibres are a reinforcement filler and a platform for the delivery of therapeutic lithium-ions.
34082941	3	32	theme	laser	591:595	arg1	spinning					597:604	laser spinning	591:604	laser spinning	591:604	Here, we reinforce hyaluronic acid (HA) hydrogels with 13-93-lithium bioactive glass micro- and nanofibres produced by laser spinning.
34082941	1	33	theme	due	213:215	arg1	challenge					203:211	a significant challenge	189:211	a significant challenge due to the low regenerative capacity of this tissue	189:263	The repair of articular cartilage lesions in weight-bearing joints remains as a significant challenge due to the low regenerative capacity of this tissue.
34082941	5	34	theme	higher	775:780	arg1	times					769:773	more than three times	753:773	more than three times higher than in HA hydrogels	753:801	The elastic modulus of the composites is more than three times higher than in HA hydrogels.
34082941	5	34	theme	higher	775:780	arg1	modulus					724:730	The elastic modulus	712:730	The elastic modulus of the composites	712:748	The elastic modulus of the composites is more than three times higher than in HA hydrogels.
34082941	5	35	theme	elastic	716:722	arg1	modulus					724:730	The elastic modulus	712:730	The elastic modulus of the composites	712:748	The elastic modulus of the composites is more than three times higher than in HA hydrogels.
34082941	5	35	theme	elastic	716:722	arg1	times					769:773	more than three times	753:773	more than three times higher than in HA hydrogels	753:801	The elastic modulus of the composites is more than three times higher than in HA hydrogels.
34082941	6	36	theme	experimental	852:863	arg1	results					865:871	the experimental results	848:871	the experimental results	848:871	Modelling of the reinforcement corroborates the experimental results.
34082941	4	37	theme	reinforcement	630:642	arg1	filler					644:649	a reinforcement filler	628:649	a reinforcement filler	628:649	The glass fibres are a reinforcement filler and a platform for the delivery of therapeutic lithium-ions.
34082941	4	37	theme	reinforcement	630:642	arg1	fibres					617:622	The glass fibres	607:622	The glass fibres	607:622	The glass fibres are a reinforcement filler and a platform for the delivery of therapeutic lithium-ions.
34082941	0	38	theme	bioactive	53:61	arg1	micro-					69:74	laser spun bioactive glass micro-	42:74	laser spun bioactive glass micro-	42:74	Hyaluronic acid hydrogels reinforced with laser spun bioactive glass micro- and nanofibres doped with lithium.
34082941	7	39	contain	containing	982:991	arg2	fibres					993:998	fibres	993:998	fibres than in HA hydrogels alone	993:1025	ATDC5 chondrogenic cells seeded on the composites are viable and more proliferation occurs on the hydrogels containing fibres than in HA hydrogels alone.
34082941	7	39	contain	containing	982:991	arg1	hydrogels					972:980	the hydrogels	968:980	the hydrogels containing fibres than in HA hydrogels alone	968:1025	ATDC5 chondrogenic cells seeded on the composites are viable and more proliferation occurs on the hydrogels containing fibres than in HA hydrogels alone.
34082941	0	40	theme	spun	48:51	arg1	micro-					69:74	laser spun bioactive glass micro-	42:74	laser spun bioactive glass micro-	42:74	Hyaluronic acid hydrogels reinforced with laser spun bioactive glass micro- and nanofibres doped with lithium.
34082941	7	41	theme	HA	1008:1009	arg1	hydrogels					1011:1019	HA hydrogels	1008:1019	HA hydrogels alone	1008:1025	ATDC5 chondrogenic cells seeded on the composites are viable and more proliferation occurs on the hydrogels containing fibres than in HA hydrogels alone.
34082941	3	42	theme	hyaluronic	491:500	arg1	HA					508:509	HA	508:509	HA	508:509	Here, we reinforce hyaluronic acid (HA) hydrogels with 13-93-lithium bioactive glass micro- and nanofibres produced by laser spinning.
34082941	3	42	theme	hyaluronic	491:500	arg1	acid					502:505	hyaluronic acid	491:505	hyaluronic acid (HA) hydrogels with 13-93-lithium bioactive glass micro- and nanofibres produced by laser spinning	491:604	Here, we reinforce hyaluronic acid (HA) hydrogels with 13-93-lithium bioactive glass micro- and nanofibres produced by laser spinning.
34082941	8	43	from	behavior	1058:1065	arg1	constructs					1073:1082	HA constructs	1070:1082	HA constructs with fibres containing lithium	1070:1113	Furthermore, the chondrogenic behavior on HA constructs with fibres containing lithium is more marked than in hydrogels with no-lithium fibres.
34082941	6	44	theme	reinforcement	821:833	arg1	Modelling					804:812	Modelling	804:812	Modelling of the reinforcement	804:833	Modelling of the reinforcement corroborates the experimental results.
34082941	2	45	theme	similar	322:328	arg1	properties					330:339	similar properties	322:339	similar properties	322:339	Hydrogels are candidates to repair lesions as they have similar properties to cartilage extracellular matrix but they are unable to meet the mechanical and biological requirements for a successful outcome.
34082941	0	46	dep	hydrogels	16:24	arg1	reinforced					26:35	reinforced	26:35	hydrogels reinforced with laser spun bioactive glass micro-	16:74	Hyaluronic acid hydrogels reinforced with laser spun bioactive glass micro- and nanofibres doped with lithium.
34082941	1	47	theme	articular	125:133	arg1	lesions					145:151	articular cartilage lesions	125:151	articular cartilage lesions	125:151	The repair of articular cartilage lesions in weight-bearing joints remains as a significant challenge due to the low regenerative capacity of this tissue.
34082941	1	48	theme	low	224:226	arg1	capacity					241:248	the low regenerative capacity	220:248	the low regenerative capacity of this tissue	220:263	The repair of articular cartilage lesions in weight-bearing joints remains as a significant challenge due to the low regenerative capacity of this tissue.
34082941	7	49	theme	chondrogenic	880:891	arg1	cells					893:897	ATDC5 chondrogenic cells	874:897	ATDC5 chondrogenic cells seeded on the composites	874:922	ATDC5 chondrogenic cells seeded on the composites are viable and more proliferation occurs on the hydrogels containing fibres than in HA hydrogels alone.
34082941	1	50	theme	cartilage	135:143	arg1	lesions					145:151	articular cartilage lesions	125:151	articular cartilage lesions	125:151	The repair of articular cartilage lesions in weight-bearing joints remains as a significant challenge due to the low regenerative capacity of this tissue.
34082941	1	51	theme	regenerative	228:239	arg1	capacity					241:248	the low regenerative capacity	220:248	the low regenerative capacity of this tissue	220:263	The repair of articular cartilage lesions in weight-bearing joints remains as a significant challenge due to the low regenerative capacity of this tissue.
34082941	0	52	theme	glass	63:67	arg1	micro-					69:74	laser spun bioactive glass micro-	42:74	laser spun bioactive glass micro-	42:74	Hyaluronic acid hydrogels reinforced with laser spun bioactive glass micro- and nanofibres doped with lithium.
34082941	2	53	contain	have	317:320	arg2	properties					330:339	similar properties	322:339	similar properties	322:339	Hydrogels are candidates to repair lesions as they have similar properties to cartilage extracellular matrix but they are unable to meet the mechanical and biological requirements for a successful outcome.
34082941	2	53	contain	have	317:320	arg1	they					312:315	they	312:315	they	312:315	Hydrogels are candidates to repair lesions as they have similar properties to cartilage extracellular matrix but they are unable to meet the mechanical and biological requirements for a successful outcome.
34082941	1	54	theme	lesions	145:151	arg1	repair					115:120	The repair	111:120	The repair of articular cartilage lesions in weight-bearing joints	111:176	The repair of articular cartilage lesions in weight-bearing joints remains as a significant challenge due to the low regenerative capacity of this tissue.
34082941	4	55	theme	glass	611:615	arg1	filler					644:649	a reinforcement filler	628:649	a reinforcement filler	628:649	The glass fibres are a reinforcement filler and a platform for the delivery of therapeutic lithium-ions.
34082941	4	55	theme	glass	611:615	arg1	fibres					617:622	The glass fibres	607:622	The glass fibres	607:622	The glass fibres are a reinforcement filler and a platform for the delivery of therapeutic lithium-ions.
34197851	1	0	theme	biocomposite	210:221	arg1	films					223:227	novel photoluminescent and transparent biocomposite films	171:227	novel photoluminescent and transparent biocomposite films based on chitosan reinforced with styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications	171:344	In the present investigation, novel photoluminescent and transparent biocomposite films based on chitosan reinforced with styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications were successfully prepared by casting solvent.
34197851	0	1	theme	anti-counterfeiting	107:125	arg1	applications					127:138	anti-counterfeiting applications	107:138	anti-counterfeiting applications	107:138	Photoluminescent biocomposite films of chitosan based on styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications.
34197851	5	2	from	value	1090:1094	arg1	material					1119:1126	anti-counterfeiting material	1099:1126	anti-counterfeiting material in large-scale	1099:1141	These photoluminescent biocomposite film hold promising applicative value in anti-counterfeiting material in large-scale.
34197851	3	3	theme	photonic	668:675	arg1	materials					677:685	the resulting fluorescent photonic materials	642:685	the resulting fluorescent photonic materials	642:685	These photochromic compounds have been used to functionalize cellulose nanocrystal and the resulting fluorescent photonic materials were characterized by FTIR, 13C-CP/MAS NMR as well as photoluminescent analysis to confirm the successful grafting.
34197851	2	4	theme	styrylbenzothiazolium	405:425	arg1	derivatives					427:437	Three novel styrylbenzothiazolium derivatives	393:437	Three novel styrylbenzothiazolium derivatives	393:437	Three novel styrylbenzothiazolium derivatives were synthesized by Knoevenagel condensation and characterized by FTIR, 1H, 13C NMR and photoluminescence analysis.
34197851	1	5	theme	casting	376:382	arg1	solvent					384:390	casting solvent	376:390	casting solvent	376:390	In the present investigation, novel photoluminescent and transparent biocomposite films based on chitosan reinforced with styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications were successfully prepared by casting solvent.
34197851	2	6	theme	novel	399:403	arg1	derivatives					427:437	Three novel styrylbenzothiazolium derivatives	393:437	Three novel styrylbenzothiazolium derivatives	393:437	Three novel styrylbenzothiazolium derivatives were synthesized by Knoevenagel condensation and characterized by FTIR, 1H, 13C NMR and photoluminescence analysis.
34197851	2	7	theme	FTIR	505:508	arg1	NMR					519:521	FTIR, 1H, 13C NMR	505:521	NMR	519:521	Three novel styrylbenzothiazolium derivatives were synthesized by Knoevenagel condensation and characterized by FTIR, 1H, 13C NMR and photoluminescence analysis.
34197851	2	8	theme	Knoevenagel	459:469	arg1	condensation					471:482	Knoevenagel condensation	459:482	Knoevenagel condensation	459:482	Three novel styrylbenzothiazolium derivatives were synthesized by Knoevenagel condensation and characterized by FTIR, 1H, 13C NMR and photoluminescence analysis.
34197851	4	9	theme	%	848:848	arg1	addition					832:839	the addition	828:839	the addition of 5 wt% of fluorescent modified CNC to chitosan matrix	828:895	It can be concluded that the addition of 5 wt% of fluorescent modified CNC to chitosan matrix increase the photoluminescent properties as well as improved the mechanical properties of the Cs/CNC-dye biocomposite films.
34197851	5	10	theme	anti-counterfeiting	1099:1117	arg1	material					1119:1126	anti-counterfeiting material	1099:1126	anti-counterfeiting material in large-scale	1099:1141	These photoluminescent biocomposite film hold promising applicative value in anti-counterfeiting material in large-scale.
34197851	4	11	theme	films	1015:1019	arg1	properties					927:936	the photoluminescent properties	906:936	the photoluminescent properties as well as improved the mechanical properties of the Cs/CNC-dye biocomposite films	906:1019	It can be concluded that the addition of 5 wt% of fluorescent modified CNC to chitosan matrix increase the photoluminescent properties as well as improved the mechanical properties of the Cs/CNC-dye biocomposite films.
34197851	4	11	theme	films	1015:1019	arg1	properties					973:982	the mechanical properties	958:982	the photoluminescent properties as well as improved the mechanical properties of the Cs/CNC-dye biocomposite films	906:1019	It can be concluded that the addition of 5 wt% of fluorescent modified CNC to chitosan matrix increase the photoluminescent properties as well as improved the mechanical properties of the Cs/CNC-dye biocomposite films.
34197851	3	12	theme	cellulose	616:624	arg1	nanocrystal					626:636	cellulose nanocrystal	616:636	cellulose nanocrystal	616:636	These photochromic compounds have been used to functionalize cellulose nanocrystal and the resulting fluorescent photonic materials were characterized by FTIR, 13C-CP/MAS NMR as well as photoluminescent analysis to confirm the successful grafting.
34197851	5	13	theme	photoluminescent	1028:1043	arg1	film					1058:1061	These photoluminescent biocomposite film	1022:1061	These photoluminescent biocomposite film	1022:1061	These photoluminescent biocomposite film hold promising applicative value in anti-counterfeiting material in large-scale.
34197851	4	14	theme	biocomposite	1002:1013	arg1	films					1015:1019	the Cs/CNC-dye biocomposite films	987:1019	the Cs/CNC-dye biocomposite films	987:1019	It can be concluded that the addition of 5 wt% of fluorescent modified CNC to chitosan matrix increase the photoluminescent properties as well as improved the mechanical properties of the Cs/CNC-dye biocomposite films.
34197851	5	15	theme	biocomposite	1045:1056	arg1	film					1058:1061	These photoluminescent biocomposite film	1022:1061	These photoluminescent biocomposite film	1022:1061	These photoluminescent biocomposite film hold promising applicative value in anti-counterfeiting material in large-scale.
34197851	0	16	theme	biocomposite	17:28	arg1	films					30:34	Photoluminescent biocomposite films	0:34	Photoluminescent biocomposite films of chitosan	0:46	Photoluminescent biocomposite films of chitosan based on styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications.
34197851	3	17	theme	photochromic	561:572	arg1	compounds					574:582	These photochromic compounds	555:582	These photochromic compounds	555:582	These photochromic compounds have been used to functionalize cellulose nanocrystal and the resulting fluorescent photonic materials were characterized by FTIR, 13C-CP/MAS NMR as well as photoluminescent analysis to confirm the successful grafting.
34197851	0	18	theme	Photoluminescent	0:15	arg1	films					30:34	Photoluminescent biocomposite films	0:34	Photoluminescent biocomposite films of chitosan	0:46	Photoluminescent biocomposite films of chitosan based on styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications.
34197851	4	19	theme	CNC	874:876	arg1	%					848:848	5 wt%	844:848	5 wt% of fluorescent modified CNC	844:876	It can be concluded that the addition of 5 wt% of fluorescent modified CNC to chitosan matrix increase the photoluminescent properties as well as improved the mechanical properties of the Cs/CNC-dye biocomposite films.
34197851	4	19	theme	CNC	874:876	arg1	CNC					874:876	fluorescent modified CNC	853:876	fluorescent modified CNC	853:876	It can be concluded that the addition of 5 wt% of fluorescent modified CNC to chitosan matrix increase the photoluminescent properties as well as improved the mechanical properties of the Cs/CNC-dye biocomposite films.
34197851	3	20	used	used	594:597	arg2	compounds					574:582	These photochromic compounds	555:582	These photochromic compounds	555:582	These photochromic compounds have been used to functionalize cellulose nanocrystal and the resulting fluorescent photonic materials were characterized by FTIR, 13C-CP/MAS NMR as well as photoluminescent analysis to confirm the successful grafting.
34197851	5	21	from	material	1119:1126	arg1	large-scale					1131:1141	large-scale	1131:1141	large-scale	1131:1141	These photoluminescent biocomposite film hold promising applicative value in anti-counterfeiting material in large-scale.
34197851	4	22	theme	chitosan	881:888	arg1	matrix					890:895	chitosan matrix	881:895	chitosan matrix	881:895	It can be concluded that the addition of 5 wt% of fluorescent modified CNC to chitosan matrix increase the photoluminescent properties as well as improved the mechanical properties of the Cs/CNC-dye biocomposite films.
34197851	1	23	theme	present	148:154	arg1	investigation					156:168	the present investigation	144:168	the present investigation	144:168	In the present investigation, novel photoluminescent and transparent biocomposite films based on chitosan reinforced with styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications were successfully prepared by casting solvent.
34197851	1	24	theme	styrylbenzothiazolium-g-cellulose	263:295	arg1	nanocrystal					297:307	styrylbenzothiazolium-g-cellulose nanocrystal	263:307	styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications	263:344	In the present investigation, novel photoluminescent and transparent biocomposite films based on chitosan reinforced with styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications were successfully prepared by casting solvent.
34197851	3	25	theme	fluorescent	656:666	arg1	materials					677:685	the resulting fluorescent photonic materials	642:685	the resulting fluorescent photonic materials	642:685	These photochromic compounds have been used to functionalize cellulose nanocrystal and the resulting fluorescent photonic materials were characterized by FTIR, 13C-CP/MAS NMR as well as photoluminescent analysis to confirm the successful grafting.
34197851	4	26	theme	fluorescent	853:863	arg1	CNC					874:876	fluorescent modified CNC	853:876	fluorescent modified CNC	853:876	It can be concluded that the addition of 5 wt% of fluorescent modified CNC to chitosan matrix increase the photoluminescent properties as well as improved the mechanical properties of the Cs/CNC-dye biocomposite films.
34197851	0	27	theme	chitosan	39:46	arg1	films					30:34	Photoluminescent biocomposite films	0:34	Photoluminescent biocomposite films of chitosan	0:46	Photoluminescent biocomposite films of chitosan based on styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications.
34197851	4	28	theme	photoluminescent	910:925	arg1	properties					927:936	the photoluminescent properties	906:936	the photoluminescent properties as well as improved the mechanical properties of the Cs/CNC-dye biocomposite films	906:1019	It can be concluded that the addition of 5 wt% of fluorescent modified CNC to chitosan matrix increase the photoluminescent properties as well as improved the mechanical properties of the Cs/CNC-dye biocomposite films.
34197851	2	29	theme	photoluminescence	527:543	arg1	analysis					545:552	photoluminescence analysis	527:552	photoluminescence analysis	527:552	Three novel styrylbenzothiazolium derivatives were synthesized by Knoevenagel condensation and characterized by FTIR, 1H, 13C NMR and photoluminescence analysis.
34197851	0	30	theme	styrylbenzothiazolium-g-cellulose	57:89	arg1	nanocrystal					91:101	styrylbenzothiazolium-g-cellulose nanocrystal	57:101	styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications	57:138	Photoluminescent biocomposite films of chitosan based on styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications.
34197851	4	31	theme	Cs/CNC-dye	991:1000	arg1	films					1015:1019	the Cs/CNC-dye biocomposite films	987:1019	the Cs/CNC-dye biocomposite films	987:1019	It can be concluded that the addition of 5 wt% of fluorescent modified CNC to chitosan matrix increase the photoluminescent properties as well as improved the mechanical properties of the Cs/CNC-dye biocomposite films.
34197851	4	32	theme	modified	865:872	arg1	CNC					874:876	fluorescent modified CNC	853:876	fluorescent modified CNC	853:876	It can be concluded that the addition of 5 wt% of fluorescent modified CNC to chitosan matrix increase the photoluminescent properties as well as improved the mechanical properties of the Cs/CNC-dye biocomposite films.
34197851	3	33	theme	photoluminescent	741:756	arg1	analysis					758:765	photoluminescent analysis	741:765	FTIR, 13C-CP/MAS NMR as well as photoluminescent analysis to confirm the successful grafting	709:800	These photochromic compounds have been used to functionalize cellulose nanocrystal and the resulting fluorescent photonic materials were characterized by FTIR, 13C-CP/MAS NMR as well as photoluminescent analysis to confirm the successful grafting.
34197851	3	34	theme	resulting	646:654	arg1	materials					677:685	the resulting fluorescent photonic materials	642:685	the resulting fluorescent photonic materials	642:685	These photochromic compounds have been used to functionalize cellulose nanocrystal and the resulting fluorescent photonic materials were characterized by FTIR, 13C-CP/MAS NMR as well as photoluminescent analysis to confirm the successful grafting.
34197851	4	35	theme	improved	949:956	arg1	properties					973:982	the mechanical properties	958:982	the photoluminescent properties as well as improved the mechanical properties of the Cs/CNC-dye biocomposite films	906:1019	It can be concluded that the addition of 5 wt% of fluorescent modified CNC to chitosan matrix increase the photoluminescent properties as well as improved the mechanical properties of the Cs/CNC-dye biocomposite films.
34197851	3	36	theme	successful	782:791	arg1	grafting					793:800	the successful grafting	778:800	the successful grafting	778:800	These photochromic compounds have been used to functionalize cellulose nanocrystal and the resulting fluorescent photonic materials were characterized by FTIR, 13C-CP/MAS NMR as well as photoluminescent analysis to confirm the successful grafting.
34197851	1	37	theme	novel	171:175	arg1	films					223:227	novel photoluminescent and transparent biocomposite films	171:227	novel photoluminescent and transparent biocomposite films based on chitosan reinforced with styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications	171:344	In the present investigation, novel photoluminescent and transparent biocomposite films based on chitosan reinforced with styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications were successfully prepared by casting solvent.
34197851	1	38	theme	anti-counterfeiting	313:331	arg1	applications					333:344	anti-counterfeiting applications	313:344	anti-counterfeiting applications	313:344	In the present investigation, novel photoluminescent and transparent biocomposite films based on chitosan reinforced with styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications were successfully prepared by casting solvent.
34197851	3	39	theme	FTIR	709:712	arg1	NMR					726:728	FTIR, 13C-CP/MAS NMR	709:728	FTIR, 13C-CP/MAS NMR as well as photoluminescent analysis to confirm the successful grafting	709:800	These photochromic compounds have been used to functionalize cellulose nanocrystal and the resulting fluorescent photonic materials were characterized by FTIR, 13C-CP/MAS NMR as well as photoluminescent analysis to confirm the successful grafting.
34197851	1	40	theme	photoluminescent	177:192	arg1	films					223:227	novel photoluminescent and transparent biocomposite films	171:227	novel photoluminescent and transparent biocomposite films based on chitosan reinforced with styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications	171:344	In the present investigation, novel photoluminescent and transparent biocomposite films based on chitosan reinforced with styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications were successfully prepared by casting solvent.
34197851	3	41	dep	FTIR	709:712	arg1	13C-CP/MAS					715:724	13C-CP/MAS	715:724	13C-CP/MAS	715:724	These photochromic compounds have been used to functionalize cellulose nanocrystal and the resulting fluorescent photonic materials were characterized by FTIR, 13C-CP/MAS NMR as well as photoluminescent analysis to confirm the successful grafting.
34197851	5	42	theme	applicative	1078:1088	arg1	value					1090:1094	promising applicative value	1068:1094	promising applicative value in anti-counterfeiting material in large-scale	1068:1141	These photoluminescent biocomposite film hold promising applicative value in anti-counterfeiting material in large-scale.
34197851	4	43	theme	mechanical	962:971	arg1	properties					973:982	the mechanical properties	958:982	the photoluminescent properties as well as improved the mechanical properties of the Cs/CNC-dye biocomposite films	906:1019	It can be concluded that the addition of 5 wt% of fluorescent modified CNC to chitosan matrix increase the photoluminescent properties as well as improved the mechanical properties of the Cs/CNC-dye biocomposite films.
34197851	5	44	theme	promising	1068:1076	arg1	value					1090:1094	promising applicative value	1068:1094	promising applicative value in anti-counterfeiting material in large-scale	1068:1141	These photoluminescent biocomposite film hold promising applicative value in anti-counterfeiting material in large-scale.
34197851	2	45	dep	NMR	519:521	arg1	13C					515:517	FTIR, 1H, 13C NMR	505:521	13C	515:517	Three novel styrylbenzothiazolium derivatives were synthesized by Knoevenagel condensation and characterized by FTIR, 1H, 13C NMR and photoluminescence analysis.
34197851	2	45	dep	NMR	519:521	arg1	1H					511:512	FTIR, 1H, 13C NMR	505:521	1H	511:512	Three novel styrylbenzothiazolium derivatives were synthesized by Knoevenagel condensation and characterized by FTIR, 1H, 13C NMR and photoluminescence analysis.
34197851	1	46	theme	transparent	198:208	arg1	films					223:227	novel photoluminescent and transparent biocomposite films	171:227	novel photoluminescent and transparent biocomposite films based on chitosan reinforced with styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications	171:344	In the present investigation, novel photoluminescent and transparent biocomposite films based on chitosan reinforced with styrylbenzothiazolium-g-cellulose nanocrystal for anti-counterfeiting applications were successfully prepared by casting solvent.
34420735	5	0	theme	detection	988:996	arg1	level					998:1002	the detection level	984:1002	the detection level	984:1002	Under different test temperatures, the nonenzymatic browning and the content of chitosan-citric complex will significantly improve the oxygen barrier property of membranes (P < 0.05), and C-Ch3-F membrane represented the value of oxygen permeation below the detection level.
34420735	5	1	theme	test	746:749	arg1	temperatures					751:762	different test temperatures	736:762	different test temperatures	736:762	Under different test temperatures, the nonenzymatic browning and the content of chitosan-citric complex will significantly improve the oxygen barrier property of membranes (P < 0.05), and C-Ch3-F membrane represented the value of oxygen permeation below the detection level.
34420735	7	2	from	application	1273:1283	arg1	packaging					1293:1301	food packaging	1288:1301	food packaging	1288:1301	The excellent decontamination in meat preservation endowed C-Chx-F films with potential application in food packaging.
34420735	2	3	from	coating	344:350	arg1	surface					386:392	the surface	382:392	the surface of cellulose	382:405	Cellulose-chitosan-citric films (C-Chx-F) were successfully obtained by a facile coating of chitosan-citric complex on the surface of cellulose.
34420735	6	4	theme	excellent	1152:1160	arg1	ability					1176:1182	excellent antibacterial ability	1152:1182	excellent antibacterial ability	1152:1182	Excellent antibacterial capability of C-Chx-F hydrogels demonstrated that polycationic chitosan-citric complex immobilized in films still retained excellent antibacterial ability.
34420735	6	5	theme	C-Chx-F	1043:1049	arg1	hydrogels					1051:1059	C-Chx-F hydrogels	1043:1059	C-Chx-F hydrogels	1043:1059	Excellent antibacterial capability of C-Chx-F hydrogels demonstrated that polycationic chitosan-citric complex immobilized in films still retained excellent antibacterial ability.
34420735	2	6	theme	cellulose	397:405	arg1	surface					386:392	the surface	382:392	the surface of cellulose	382:405	Cellulose-chitosan-citric films (C-Chx-F) were successfully obtained by a facile coating of chitosan-citric complex on the surface of cellulose.
34420735	4	7	theme	module	658:663	arg1	stress					619:624	excellent breaking stress	600:624	excellent breaking stress of 6.03 ± 0.25 MPa, and elastic module of 27.09 ± 1.21 MPa, probably assigned to nonenzymatic browning	600:727	C-Ch3-F hydrogel showed excellent breaking stress of 6.03 ± 0.25 MPa, and elastic module of 27.09 ± 1.21 MPa, probably assigned to nonenzymatic browning.
34420735	0	8	theme	browning	104:111	arg1	Influence					78:86	Influence	78:86	Influence of nonenzymatic browning	78:111	Cellulosic films reinforced by chitosan-citric complex for meat preservation: Influence of nonenzymatic browning.
34420735	6	9	theme	antibacterial	1015:1027	arg1	capability					1029:1038	Excellent antibacterial capability	1005:1038	Excellent antibacterial capability of C-Chx-F hydrogels	1005:1059	Excellent antibacterial capability of C-Chx-F hydrogels demonstrated that polycationic chitosan-citric complex immobilized in films still retained excellent antibacterial ability.
34420735	7	10	theme	food	1288:1291	arg1	packaging					1293:1301	food packaging	1288:1301	food packaging	1288:1301	The excellent decontamination in meat preservation endowed C-Chx-F films with potential application in food packaging.
34420735	2	11	theme	facile	337:342	arg1	coating					344:350	a facile coating	335:350	a facile coating of chitosan-citric complex on the surface of cellulose	335:405	Cellulose-chitosan-citric films (C-Chx-F) were successfully obtained by a facile coating of chitosan-citric complex on the surface of cellulose.
34420735	0	12	theme	nonenzymatic	91:102	arg1	browning					104:111	nonenzymatic browning	91:111	nonenzymatic browning	91:111	Cellulosic films reinforced by chitosan-citric complex for meat preservation: Influence of nonenzymatic browning.
34420735	6	13	theme	Excellent	1005:1013	arg1	capability					1029:1038	Excellent antibacterial capability	1005:1038	Excellent antibacterial capability of C-Chx-F hydrogels	1005:1059	Excellent antibacterial capability of C-Chx-F hydrogels demonstrated that polycationic chitosan-citric complex immobilized in films still retained excellent antibacterial ability.
34420735	4	14	theme	27.09 ± 1.21 MPa	668:683	arg1	module					658:663	elastic module	650:663	elastic module of 27.09 ± 1.21 MPa	650:683	C-Ch3-F hydrogel showed excellent breaking stress of 6.03 ± 0.25 MPa, and elastic module of 27.09 ± 1.21 MPa, probably assigned to nonenzymatic browning.
34420735	4	14	theme	27.09 ± 1.21 MPa	668:683	arg1	6.03 ± 0.25 MPa					629:643	6.03 ± 0.25 MPa	629:643	6.03 ± 0.25 MPa	629:643	C-Ch3-F hydrogel showed excellent breaking stress of 6.03 ± 0.25 MPa, and elastic module of 27.09 ± 1.21 MPa, probably assigned to nonenzymatic browning.
34420735	7	15	with	endowed	1236:1242	arg1	application					1273:1283	potential application	1263:1283	potential application in food packaging	1263:1301	The excellent decontamination in meat preservation endowed C-Chx-F films with potential application in food packaging.
34420735	1	16	dep	methods	118:124	arg1	obtain					129:134	obtain	129:134	to obtain cellulose-chitosan composite films exhibiting excellent water-resisting and antibacterial abilities	126:234	The methods to obtain cellulose-chitosan composite films exhibiting excellent water-resisting and antibacterial abilities have been widely explored.
34420735	1	17	theme	water-resisting	192:206	arg1	abilities					226:234	excellent water-resisting and antibacterial abilities	182:234	excellent water-resisting and antibacterial abilities	182:234	The methods to obtain cellulose-chitosan composite films exhibiting excellent water-resisting and antibacterial abilities have been widely explored.
34420735	7	18	from	decontamination	1199:1213	arg1	preservation					1223:1234	meat preservation	1218:1234	meat preservation	1218:1234	The excellent decontamination in meat preservation endowed C-Chx-F films with potential application in food packaging.
34420735	5	19	theme	permeation	967:976	arg1	value					951:955	the value	947:955	the value of oxygen permeation	947:976	Under different test temperatures, the nonenzymatic browning and the content of chitosan-citric complex will significantly improve the oxygen barrier property of membranes (P < 0.05), and C-Ch3-F membrane represented the value of oxygen permeation below the detection level.
34420735	3	20	theme	browning	439:446	arg1	occurrence					412:421	The occurrence	408:421	The occurrence of nonenzymatic browning at 80 °C	408:455	The occurrence of nonenzymatic browning at 80 °C improved the thermal stability, water-resistance, mechanical property and oxygen-barrier ability of C-Chx-F membranes.
34420735	2	21	theme	complex	371:377	arg1	coating					344:350	a facile coating	335:350	a facile coating of chitosan-citric complex on the surface of cellulose	335:405	Cellulose-chitosan-citric films (C-Chx-F) were successfully obtained by a facile coating of chitosan-citric complex on the surface of cellulose.
34420735	7	22	theme	meat	1218:1221	arg1	preservation					1223:1234	meat preservation	1218:1234	meat preservation	1218:1234	The excellent decontamination in meat preservation endowed C-Chx-F films with potential application in food packaging.
34420735	1	23	theme	antibacterial	212:224	arg1	abilities					226:234	excellent water-resisting and antibacterial abilities	182:234	excellent water-resisting and antibacterial abilities	182:234	The methods to obtain cellulose-chitosan composite films exhibiting excellent water-resisting and antibacterial abilities have been widely explored.
34420735	0	24	theme	Cellulosic	0:9	arg1	films					11:15	Cellulosic films	0:15	Cellulosic films	0:15	Cellulosic films reinforced by chitosan-citric complex for meat preservation: Influence of nonenzymatic browning.
34420735	5	25	theme	different	736:744	arg1	temperatures					751:762	different test temperatures	736:762	different test temperatures	736:762	Under different test temperatures, the nonenzymatic browning and the content of chitosan-citric complex will significantly improve the oxygen barrier property of membranes (P < 0.05), and C-Ch3-F membrane represented the value of oxygen permeation below the detection level.
34420735	5	26	theme	oxygen	960:965	arg1	permeation					967:976	oxygen permeation	960:976	oxygen permeation	960:976	Under different test temperatures, the nonenzymatic browning and the content of chitosan-citric complex will significantly improve the oxygen barrier property of membranes (P < 0.05), and C-Ch3-F membrane represented the value of oxygen permeation below the detection level.
34420735	2	27	theme	chitosan-citric	355:369	arg1	complex					371:377	chitosan-citric complex	355:377	chitosan-citric complex	355:377	Cellulose-chitosan-citric films (C-Chx-F) were successfully obtained by a facile coating of chitosan-citric complex on the surface of cellulose.
34420735	5	28	theme	C-Ch3-F	918:924	arg1	membrane					926:933	C-Ch3-F membrane	918:933	C-Ch3-F membrane	918:933	Under different test temperatures, the nonenzymatic browning and the content of chitosan-citric complex will significantly improve the oxygen barrier property of membranes (P < 0.05), and C-Ch3-F membrane represented the value of oxygen permeation below the detection level.
34420735	4	29	theme	6.03 ± 0.25 MPa	629:643	arg1	stress					619:624	excellent breaking stress	600:624	excellent breaking stress of 6.03 ± 0.25 MPa, and elastic module of 27.09 ± 1.21 MPa, probably assigned to nonenzymatic browning	600:727	C-Ch3-F hydrogel showed excellent breaking stress of 6.03 ± 0.25 MPa, and elastic module of 27.09 ± 1.21 MPa, probably assigned to nonenzymatic browning.
34420735	3	30	theme	nonenzymatic	426:437	arg1	browning					439:446	nonenzymatic browning	426:446	nonenzymatic browning	426:446	The occurrence of nonenzymatic browning at 80 °C improved the thermal stability, water-resistance, mechanical property and oxygen-barrier ability of C-Chx-F membranes.
34420735	4	31	theme	elastic	650:656	arg1	module					658:663	elastic module	650:663	elastic module of 27.09 ± 1.21 MPa	650:683	C-Ch3-F hydrogel showed excellent breaking stress of 6.03 ± 0.25 MPa, and elastic module of 27.09 ± 1.21 MPa, probably assigned to nonenzymatic browning.
34420735	1	32	theme	excellent	182:190	arg1	abilities					226:234	excellent water-resisting and antibacterial abilities	182:234	excellent water-resisting and antibacterial abilities	182:234	The methods to obtain cellulose-chitosan composite films exhibiting excellent water-resisting and antibacterial abilities have been widely explored.
34420735	3	33	theme	oxygen-barrier	531:544	arg1	ability					546:552	oxygen-barrier ability	531:552	oxygen-barrier ability	531:552	The occurrence of nonenzymatic browning at 80 °C improved the thermal stability, water-resistance, mechanical property and oxygen-barrier ability of C-Chx-F membranes.
34420735	5	34	theme	membranes	892:900	arg1	property					880:887	the oxygen barrier property	861:887	the oxygen barrier property of membranes (P < 0.05)	861:911	Under different test temperatures, the nonenzymatic browning and the content of chitosan-citric complex will significantly improve the oxygen barrier property of membranes (P < 0.05), and C-Ch3-F membrane represented the value of oxygen permeation below the detection level.
34420735	6	35	theme	chitosan-citric	1092:1106	arg1	complex					1108:1114	polycationic chitosan-citric complex	1079:1114	polycationic chitosan-citric complex immobilized in films	1079:1135	Excellent antibacterial capability of C-Chx-F hydrogels demonstrated that polycationic chitosan-citric complex immobilized in films still retained excellent antibacterial ability.
34420735	5	36	theme	chitosan-citric	810:824	arg1	complex					826:832	chitosan-citric complex	810:832	chitosan-citric complex	810:832	Under different test temperatures, the nonenzymatic browning and the content of chitosan-citric complex will significantly improve the oxygen barrier property of membranes (P < 0.05), and C-Ch3-F membrane represented the value of oxygen permeation below the detection level.
34420735	3	37	dep	stability	478:486	arg1	the					466:468	the	466:468	the	466:468	The occurrence of nonenzymatic browning at 80 °C improved the thermal stability, water-resistance, mechanical property and oxygen-barrier ability of C-Chx-F membranes.
34420735	3	38	theme	mechanical	507:516	arg1	property					518:525	mechanical property	507:525	mechanical property	507:525	The occurrence of nonenzymatic browning at 80 °C improved the thermal stability, water-resistance, mechanical property and oxygen-barrier ability of C-Chx-F membranes.
34420735	0	39	theme	chitosan-citric	31:45	arg1	complex					47:53	chitosan-citric complex	31:53	chitosan-citric complex for meat preservation	31:75	Cellulosic films reinforced by chitosan-citric complex for meat preservation: Influence of nonenzymatic browning.
34420735	4	40	theme	breaking	610:617	arg1	stress					619:624	excellent breaking stress	600:624	excellent breaking stress of 6.03 ± 0.25 MPa, and elastic module of 27.09 ± 1.21 MPa, probably assigned to nonenzymatic browning	600:727	C-Ch3-F hydrogel showed excellent breaking stress of 6.03 ± 0.25 MPa, and elastic module of 27.09 ± 1.21 MPa, probably assigned to nonenzymatic browning.
34420735	3	41	theme	thermal	470:476	arg1	stability					478:486	thermal stability	470:486	thermal stability	470:486	The occurrence of nonenzymatic browning at 80 °C improved the thermal stability, water-resistance, mechanical property and oxygen-barrier ability of C-Chx-F membranes.
34420735	0	42	theme	meat	59:62	arg1	preservation					64:75	meat preservation	59:75	meat preservation	59:75	Cellulosic films reinforced by chitosan-citric complex for meat preservation: Influence of nonenzymatic browning.
34420735	4	43	theme	excellent	600:608	arg1	stress					619:624	excellent breaking stress	600:624	excellent breaking stress of 6.03 ± 0.25 MPa, and elastic module of 27.09 ± 1.21 MPa, probably assigned to nonenzymatic browning	600:727	C-Ch3-F hydrogel showed excellent breaking stress of 6.03 ± 0.25 MPa, and elastic module of 27.09 ± 1.21 MPa, probably assigned to nonenzymatic browning.
34420735	5	44	theme	nonenzymatic	769:780	arg1	browning					782:789	the nonenzymatic browning	765:789	the nonenzymatic browning	765:789	Under different test temperatures, the nonenzymatic browning and the content of chitosan-citric complex will significantly improve the oxygen barrier property of membranes (P < 0.05), and C-Ch3-F membrane represented the value of oxygen permeation below the detection level.
34420735	2	45	theme	Cellulose-chitosan-citric	263:287	arg1	films					289:293	Cellulose-chitosan-citric films	263:293	Cellulose-chitosan-citric films (C-Chx-F)	263:303	Cellulose-chitosan-citric films (C-Chx-F) were successfully obtained by a facile coating of chitosan-citric complex on the surface of cellulose.
34420735	2	45	theme	Cellulose-chitosan-citric	263:287	arg1	C-Chx-F					296:302	C-Chx-F	296:302	C-Chx-F	296:302	Cellulose-chitosan-citric films (C-Chx-F) were successfully obtained by a facile coating of chitosan-citric complex on the surface of cellulose.
34420735	7	46	theme	excellent	1189:1197	arg1	decontamination					1199:1213	The excellent decontamination	1185:1213	The excellent decontamination in meat preservation	1185:1234	The excellent decontamination in meat preservation endowed C-Chx-F films with potential application in food packaging.
34420735	3	47	theme	C-Chx-F	557:563	arg1	membranes					565:573	C-Chx-F membranes	557:573	C-Chx-F membranes	557:573	The occurrence of nonenzymatic browning at 80 °C improved the thermal stability, water-resistance, mechanical property and oxygen-barrier ability of C-Chx-F membranes.
34420735	5	48	theme	complex	826:832	arg1	browning					782:789	the nonenzymatic browning	765:789	the nonenzymatic browning	765:789	Under different test temperatures, the nonenzymatic browning and the content of chitosan-citric complex will significantly improve the oxygen barrier property of membranes (P < 0.05), and C-Ch3-F membrane represented the value of oxygen permeation below the detection level.
34420735	5	48	theme	complex	826:832	arg1	content					799:805	the content	795:805	the content of chitosan-citric complex	795:832	Under different test temperatures, the nonenzymatic browning and the content of chitosan-citric complex will significantly improve the oxygen barrier property of membranes (P < 0.05), and C-Ch3-F membrane represented the value of oxygen permeation below the detection level.
34420735	4	49	theme	C-Ch3-F	576:582	arg1	hydrogel					584:591	C-Ch3-F hydrogel	576:591	C-Ch3-F hydrogel	576:591	C-Ch3-F hydrogel showed excellent breaking stress of 6.03 ± 0.25 MPa, and elastic module of 27.09 ± 1.21 MPa, probably assigned to nonenzymatic browning.
34420735	3	50	theme	membranes	565:573	arg1	water-resistance					489:504	water-resistance	489:504	water-resistance	489:504	The occurrence of nonenzymatic browning at 80 °C improved the thermal stability, water-resistance, mechanical property and oxygen-barrier ability of C-Chx-F membranes.
34420735	3	50	theme	membranes	565:573	arg1	property					518:525	mechanical property	507:525	mechanical property	507:525	The occurrence of nonenzymatic browning at 80 °C improved the thermal stability, water-resistance, mechanical property and oxygen-barrier ability of C-Chx-F membranes.
34420735	3	50	theme	membranes	565:573	arg1	stability					478:486	thermal stability	470:486	thermal stability	470:486	The occurrence of nonenzymatic browning at 80 °C improved the thermal stability, water-resistance, mechanical property and oxygen-barrier ability of C-Chx-F membranes.
34420735	3	50	theme	membranes	565:573	arg1	ability					546:552	oxygen-barrier ability	531:552	oxygen-barrier ability	531:552	The occurrence of nonenzymatic browning at 80 °C improved the thermal stability, water-resistance, mechanical property and oxygen-barrier ability of C-Chx-F membranes.
34420735	6	51	theme	hydrogels	1051:1059	arg1	capability					1029:1038	Excellent antibacterial capability	1005:1038	Excellent antibacterial capability of C-Chx-F hydrogels	1005:1059	Excellent antibacterial capability of C-Chx-F hydrogels demonstrated that polycationic chitosan-citric complex immobilized in films still retained excellent antibacterial ability.
34420735	6	52	theme	polycationic	1079:1090	arg1	complex					1108:1114	polycationic chitosan-citric complex	1079:1114	polycationic chitosan-citric complex immobilized in films	1079:1135	Excellent antibacterial capability of C-Chx-F hydrogels demonstrated that polycationic chitosan-citric complex immobilized in films still retained excellent antibacterial ability.
34420735	1	53	theme	cellulose-chitosan	136:153	arg1	films					165:169	cellulose-chitosan composite films	136:169	cellulose-chitosan composite films exhibiting excellent water-resisting and antibacterial abilities	136:234	The methods to obtain cellulose-chitosan composite films exhibiting excellent water-resisting and antibacterial abilities have been widely explored.
34420735	7	54	theme	potential	1263:1271	arg1	application					1273:1283	potential application	1263:1283	potential application in food packaging	1263:1301	The excellent decontamination in meat preservation endowed C-Chx-F films with potential application in food packaging.
34420735	5	55	theme	oxygen	865:870	arg1	property					880:887	the oxygen barrier property	861:887	the oxygen barrier property of membranes (P < 0.05)	861:911	Under different test temperatures, the nonenzymatic browning and the content of chitosan-citric complex will significantly improve the oxygen barrier property of membranes (P < 0.05), and C-Ch3-F membrane represented the value of oxygen permeation below the detection level.
34420735	1	56	theme	composite	155:163	arg1	films					165:169	cellulose-chitosan composite films	136:169	cellulose-chitosan composite films exhibiting excellent water-resisting and antibacterial abilities	136:234	The methods to obtain cellulose-chitosan composite films exhibiting excellent water-resisting and antibacterial abilities have been widely explored.
34420735	4	57	theme	nonenzymatic	707:718	arg1	browning					720:727	nonenzymatic browning	707:727	nonenzymatic browning	707:727	C-Ch3-F hydrogel showed excellent breaking stress of 6.03 ± 0.25 MPa, and elastic module of 27.09 ± 1.21 MPa, probably assigned to nonenzymatic browning.
34420735	5	58	theme	barrier	872:878	arg1	property					880:887	the oxygen barrier property	861:887	the oxygen barrier property of membranes (P < 0.05)	861:911	Under different test temperatures, the nonenzymatic browning and the content of chitosan-citric complex will significantly improve the oxygen barrier property of membranes (P < 0.05), and C-Ch3-F membrane represented the value of oxygen permeation below the detection level.
34420735	3	59	from	80 °C	451:455	arg1	occurrence					412:421	The occurrence	408:421	The occurrence of nonenzymatic browning at 80 °C	408:455	The occurrence of nonenzymatic browning at 80 °C improved the thermal stability, water-resistance, mechanical property and oxygen-barrier ability of C-Chx-F membranes.
34420735	6	60	theme	antibacterial	1162:1174	arg1	ability					1176:1182	excellent antibacterial ability	1152:1182	excellent antibacterial ability	1152:1182	Excellent antibacterial capability of C-Chx-F hydrogels demonstrated that polycationic chitosan-citric complex immobilized in films still retained excellent antibacterial ability.
33675824	0	0	theme	starch	84:89	arg1	nanoparticles					91:103	starch nanoparticles	84:103	starch nanoparticles	84:103	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.
33675824	4	1	from	increase	881:888	arg1	properties					908:917	the mechanical properties	893:917	the mechanical properties of films	893:926	The chitosan composite films were reinforced by esterified starch with different ultrasound treatment times, the results indicated that the addition of starch nanoparticles resulted in a significant increase in the mechanical properties of films.
33675824	4	2	theme	different	753:761	arg1	times					784:788	different ultrasound treatment times	753:788	different ultrasound treatment times	753:788	The chitosan composite films were reinforced by esterified starch with different ultrasound treatment times, the results indicated that the addition of starch nanoparticles resulted in a significant increase in the mechanical properties of films.
33675824	0	3	from	characteristics	158:172	arg1	properties					70:79	properties	70:79	properties	70:79	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.
33675824	0	3	from	characteristics	158:172	arg1	formation					56:64	formation	56:64	formation	56:64	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.
33675824	5	4	theme	nanocomposite	1136:1148	arg1	films					1150:1154	nanocomposite films	1136:1154	nanocomposite films	1136:1154	This study indicates that esterification and ultrasound treatment can be used to prepare starch nanoparticles with a higher crystallinity and higher efficiency, which will further promote the application of nanocomposite films in packaging applications.
33675824	4	5	theme	treatment	774:782	arg1	times					784:788	different ultrasound treatment times	753:788	different ultrasound treatment times	753:788	The chitosan composite films were reinforced by esterified starch with different ultrasound treatment times, the results indicated that the addition of starch nanoparticles resulted in a significant increase in the mechanical properties of films.
33675824	5	6	theme	higher	1071:1076	arg1	efficiency					1078:1087	higher efficiency	1071:1087	higher efficiency	1071:1087	This study indicates that esterification and ultrasound treatment can be used to prepare starch nanoparticles with a higher crystallinity and higher efficiency, which will further promote the application of nanocomposite films in packaging applications.
33675824	0	7	from	Influence	0:8	arg1	properties					70:79	properties	70:79	properties	70:79	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.
33675824	0	7	from	Influence	0:8	arg1	formation					56:64	formation	56:64	formation	56:64	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.
33675824	4	8	theme	significant	869:879	arg1	increase					881:888	a significant increase	867:888	a significant increase in the mechanical properties of films	867:926	The chitosan composite films were reinforced by esterified starch with different ultrasound treatment times, the results indicated that the addition of starch nanoparticles resulted in a significant increase in the mechanical properties of films.
33675824	0	9	theme	nanoparticles	91:103	arg1	properties					70:79	properties	70:79	properties	70:79	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.
33675824	0	9	theme	nanoparticles	91:103	arg1	formation					56:64	formation	56:64	formation	56:64	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.
33675824	0	10	from	formation	56:64	arg1	characteristics					158:172	chitosan based films characteristics	137:172	chitosan based films characteristics	137:172	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.
33675824	2	11	dep	size	461:464	arg1	a					454:454	a	454:454	a	454:454	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	2	12	theme	time	304:307	arg1	increase					271:278	the increase	267:278	the increase of ultrasonic treatment time	267:307	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	4	13	theme	composite	695:703	arg1	films					705:709	The chitosan composite films	682:709	The chitosan composite films	682:709	The chitosan composite films were reinforced by esterified starch with different ultrasound treatment times, the results indicated that the addition of starch nanoparticles resulted in a significant increase in the mechanical properties of films.
33675824	0	14	theme	impact	115:120	arg1	properties					70:79	properties	70:79	properties	70:79	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.
33675824	0	14	theme	impact	115:120	arg1	formation					56:64	formation	56:64	formation	56:64	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.
33675824	4	15	theme	chitosan	686:693	arg1	films					705:709	The chitosan composite films	682:709	The chitosan composite films	682:709	The chitosan composite films were reinforced by esterified starch with different ultrasound treatment times, the results indicated that the addition of starch nanoparticles resulted in a significant increase in the mechanical properties of films.
33675824	2	16	contain	had	450:452	arg1	nanoparticles					436:448	the prepared starch nanoparticles	416:448	the prepared starch nanoparticles	416:448	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	2	16	contain	had	450:452	arg2	PDI					470:472	PDI	470:472	PDI	470:472	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	2	16	contain	had	450:452	arg2	size					461:464	mean size	456:464	mean size	456:464	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	2	17	theme	treatment	294:302	arg1	time					304:307	ultrasonic treatment time	283:307	ultrasonic treatment time	283:307	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	2	18	theme	0.292	490:494	arg1	PDI					470:472	PDI	470:472	PDI	470:472	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	2	18	theme	0.292	490:494	arg1	size					461:464	mean size	456:464	mean size	456:464	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	2	19	theme	ultrasonic	283:292	arg1	time					304:307	ultrasonic treatment time	283:307	ultrasonic treatment time	283:307	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	1	20	theme	Starch	175:180	arg1	nanoparticles					182:194	Starch nanoparticles	175:194	Starch nanoparticles	175:194	Starch nanoparticles were prepared by citrate esterification and ultrasound treatment.
33675824	4	21	theme	nanoparticles	841:853	arg1	addition					822:829	the addition	818:829	the addition of starch nanoparticles	818:853	The chitosan composite films were reinforced by esterified starch with different ultrasound treatment times, the results indicated that the addition of starch nanoparticles resulted in a significant increase in the mechanical properties of films.
33675824	2	22	theme	ultrasonic	379:388	arg1	5 min					409:413	5 min	409:413	5 min	409:413	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	2	22	theme	ultrasonic	379:388	arg1	time					400:403	the ultrasonic treatment time	375:403	the ultrasonic treatment time	375:403	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	4	23	theme	starch	834:839	arg1	nanoparticles					841:853	starch nanoparticles	834:853	starch nanoparticles	834:853	The chitosan composite films were reinforced by esterified starch with different ultrasound treatment times, the results indicated that the addition of starch nanoparticles resulted in a significant increase in the mechanical properties of films.
33675824	2	24	theme	starch	429:434	arg1	nanoparticles					436:448	the prepared starch nanoparticles	416:448	the prepared starch nanoparticles	416:448	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	2	25	theme	mean	314:317	arg1	size					319:322	mean size	314:322	mean size	314:322	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	0	26	theme	esterification	13:26	arg1	Influence					0:8	Influence	0:8	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.	0:173	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.
33675824	3	27	theme	X-ray	511:515	arg1	XRD					530:532	XRD	530:532	XRD	530:532	X-ray diffraction (XRD) showed that the starch nanoparticles prepared by ultrasonic treatment for 5 min had an A-type crystalline structure and a crystallinity of 41.42%.
33675824	3	27	theme	X-ray	511:515	arg1	diffraction					517:527	X-ray diffraction	511:527	X-ray diffraction (XRD)	511:533	X-ray diffraction (XRD) showed that the starch nanoparticles prepared by ultrasonic treatment for 5 min had an A-type crystalline structure and a crystallinity of 41.42%.
33675824	3	28	theme	A-type	622:627	arg1	structure					641:649	an A-type crystalline structure	619:649	an A-type crystalline structure	619:649	X-ray diffraction (XRD) showed that the starch nanoparticles prepared by ultrasonic treatment for 5 min had an A-type crystalline structure and a crystallinity of 41.42%.
33675824	3	29	contain	had	615:617	arg2	crystallinity					657:669	a crystallinity	655:669	a crystallinity of 41.42%	655:679	X-ray diffraction (XRD) showed that the starch nanoparticles prepared by ultrasonic treatment for 5 min had an A-type crystalline structure and a crystallinity of 41.42%.
33675824	3	29	contain	had	615:617	arg1	nanoparticles					558:570	the starch nanoparticles	547:570	the starch nanoparticles prepared by ultrasonic treatment for 5 min	547:613	X-ray diffraction (XRD) showed that the starch nanoparticles prepared by ultrasonic treatment for 5 min had an A-type crystalline structure and a crystallinity of 41.42%.
33675824	3	29	contain	had	615:617	arg2	structure					641:649	an A-type crystalline structure	619:649	an A-type crystalline structure	619:649	X-ray diffraction (XRD) showed that the starch nanoparticles prepared by ultrasonic treatment for 5 min had an A-type crystalline structure and a crystallinity of 41.42%.
33675824	0	30	theme	treatment	43:51	arg1	Influence					0:8	Influence	0:8	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.	0:173	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.
33675824	4	31	with	starch	741:746	arg1	times					784:788	different ultrasound treatment times	753:788	different ultrasound treatment times	753:788	The chitosan composite films were reinforced by esterified starch with different ultrasound treatment times, the results indicated that the addition of starch nanoparticles resulted in a significant increase in the mechanical properties of films.
33675824	3	32	theme	crystalline	629:639	arg1	structure					641:649	an A-type crystalline structure	619:649	an A-type crystalline structure	619:649	X-ray diffraction (XRD) showed that the starch nanoparticles prepared by ultrasonic treatment for 5 min had an A-type crystalline structure and a crystallinity of 41.42%.
33675824	1	33	theme	citrate	213:219	arg1	esterification					221:234	citrate esterification	213:234	citrate esterification	213:234	Starch nanoparticles were prepared by citrate esterification and ultrasound treatment.
33675824	0	34	theme	ultrasound	32:41	arg1	treatment					43:51	ultrasound treatment	32:51	ultrasound treatment	32:51	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.
33675824	4	35	theme	films	922:926	arg1	properties					908:917	the mechanical properties	893:917	the mechanical properties of films	893:926	The chitosan composite films were reinforced by esterified starch with different ultrasound treatment times, the results indicated that the addition of starch nanoparticles resulted in a significant increase in the mechanical properties of films.
33675824	0	36	from	properties	70:79	arg1	characteristics					158:172	chitosan based films characteristics	137:172	chitosan based films characteristics	137:172	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.
33675824	5	37	theme	starch	1018:1023	arg1	nanoparticles					1025:1037	starch nanoparticles	1018:1037	starch nanoparticles	1018:1037	This study indicates that esterification and ultrasound treatment can be used to prepare starch nanoparticles with a higher crystallinity and higher efficiency, which will further promote the application of nanocomposite films in packaging applications.
33675824	2	38	theme	mean	456:459	arg1	size					461:464	mean size	456:464	mean size	456:464	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	5	39	theme	packaging	1159:1167	arg1	applications					1169:1180	packaging applications	1159:1180	packaging applications	1159:1180	This study indicates that esterification and ultrasound treatment can be used to prepare starch nanoparticles with a higher crystallinity and higher efficiency, which will further promote the application of nanocomposite films in packaging applications.
33675824	0	40	theme	based	146:150	arg1	characteristics					158:172	chitosan based films characteristics	137:172	chitosan based films characteristics	137:172	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.
33675824	5	41	theme	ultrasound	974:983	arg1	treatment					985:993	ultrasound treatment	974:993	ultrasound treatment	974:993	This study indicates that esterification and ultrasound treatment can be used to prepare starch nanoparticles with a higher crystallinity and higher efficiency, which will further promote the application of nanocomposite films in packaging applications.
33675824	3	42	theme	starch	551:556	arg1	nanoparticles					558:570	the starch nanoparticles	547:570	the starch nanoparticles prepared by ultrasonic treatment for 5 min	547:613	X-ray diffraction (XRD) showed that the starch nanoparticles prepared by ultrasonic treatment for 5 min had an A-type crystalline structure and a crystallinity of 41.42%.
33675824	2	43	theme	particles	339:347	arg1	PDI					328:330	PDI	328:330	PDI	328:330	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	2	43	theme	particles	339:347	arg1	size					319:322	mean size	314:322	mean size	314:322	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	5	44	theme	films	1150:1154	arg1	application					1121:1131	the application	1117:1131	the application of nanocomposite films in packaging applications	1117:1180	This study indicates that esterification and ultrasound treatment can be used to prepare starch nanoparticles with a higher crystallinity and higher efficiency, which will further promote the application of nanocomposite films in packaging applications.
33675824	5	45	from	application	1121:1131	arg1	applications					1169:1180	packaging applications	1159:1180	packaging applications	1159:1180	This study indicates that esterification and ultrasound treatment can be used to prepare starch nanoparticles with a higher crystallinity and higher efficiency, which will further promote the application of nanocomposite films in packaging applications.
33675824	0	46	from	impact	115:120	arg1	characteristics					158:172	chitosan based films characteristics	137:172	chitosan based films characteristics	137:172	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.
33675824	5	47	used	used	1002:1005	arg2	treatment					985:993	ultrasound treatment	974:993	ultrasound treatment	974:993	This study indicates that esterification and ultrasound treatment can be used to prepare starch nanoparticles with a higher crystallinity and higher efficiency, which will further promote the application of nanocomposite films in packaging applications.
33675824	5	47	used	used	1002:1005	arg2	esterification					955:968	esterification	955:968	esterification	955:968	This study indicates that esterification and ultrasound treatment can be used to prepare starch nanoparticles with a higher crystallinity and higher efficiency, which will further promote the application of nanocomposite films in packaging applications.
33675824	4	48	theme	mechanical	897:906	arg1	properties					908:917	the mechanical properties	893:917	the mechanical properties of films	893:926	The chitosan composite films were reinforced by esterified starch with different ultrasound treatment times, the results indicated that the addition of starch nanoparticles resulted in a significant increase in the mechanical properties of films.
33675824	5	49	theme	higher	1046:1051	arg1	crystallinity					1053:1065	a higher crystallinity	1044:1065	a higher crystallinity	1044:1065	This study indicates that esterification and ultrasound treatment can be used to prepare starch nanoparticles with a higher crystallinity and higher efficiency, which will further promote the application of nanocomposite films in packaging applications.
33675824	0	50	dep	based	146:150	arg1	chitosan					137:144	chitosan	137:144	chitosan	137:144	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.
33675824	4	51	theme	esterified	730:739	arg1	starch					741:746	esterified starch	730:746	esterified starch with different ultrasound treatment times	730:788	The chitosan composite films were reinforced by esterified starch with different ultrasound treatment times, the results indicated that the addition of starch nanoparticles resulted in a significant increase in the mechanical properties of films.
33675824	0	52	theme	films	152:156	arg1	characteristics					158:172	chitosan based films characteristics	137:172	chitosan based films characteristics	137:172	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.
33675824	4	53	theme	ultrasound	763:772	arg1	times					784:788	different ultrasound treatment times	753:788	different ultrasound treatment times	753:788	The chitosan composite films were reinforced by esterified starch with different ultrasound treatment times, the results indicated that the addition of starch nanoparticles resulted in a significant increase in the mechanical properties of films.
33675824	2	54	dep	size	319:322	arg1	the					310:312	the	310:312	the	310:312	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	1	55	theme	ultrasound	240:249	arg1	treatment					251:259	ultrasound treatment	240:259	ultrasound treatment	240:259	Starch nanoparticles were prepared by citrate esterification and ultrasound treatment.
33675824	0	56	from	nanoparticles	91:103	arg1	characteristics					158:172	chitosan based films characteristics	137:172	chitosan based films characteristics	137:172	Influence of esterification and ultrasound treatment on formation and properties of starch nanoparticles and their impact as a filler on chitosan based films characteristics.
33675824	3	57	theme	ultrasonic	584:593	arg1	treatment					595:603	ultrasonic treatment	584:603	ultrasonic treatment for 5 min	584:613	X-ray diffraction (XRD) showed that the starch nanoparticles prepared by ultrasonic treatment for 5 min had an A-type crystalline structure and a crystallinity of 41.42%.
33675824	2	58	theme	treatment	390:398	arg1	5 min					409:413	5 min	409:413	5 min	409:413	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	2	58	theme	treatment	390:398	arg1	time					400:403	the ultrasonic treatment time	375:403	the ultrasonic treatment time	375:403	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	2	59	theme	352.8 nm	477:484	arg1	PDI					470:472	PDI	470:472	PDI	470:472	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	2	59	theme	352.8 nm	477:484	arg1	size					461:464	mean size	456:464	mean size	456:464	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
33675824	3	60	theme	%	679:679	arg1	structure					641:649	an A-type crystalline structure	619:649	an A-type crystalline structure	619:649	X-ray diffraction (XRD) showed that the starch nanoparticles prepared by ultrasonic treatment for 5 min had an A-type crystalline structure and a crystallinity of 41.42%.
33675824	3	60	theme	%	679:679	arg1	crystallinity					657:669	a crystallinity	655:669	a crystallinity of 41.42%	655:679	X-ray diffraction (XRD) showed that the starch nanoparticles prepared by ultrasonic treatment for 5 min had an A-type crystalline structure and a crystallinity of 41.42%.
33675824	2	61	theme	prepared	420:427	arg1	nanoparticles					436:448	the prepared starch nanoparticles	416:448	the prepared starch nanoparticles	416:448	With the increase of ultrasonic treatment time, the mean size and PDI of the particles decreased gradually, when the ultrasonic treatment time was 5 min, the prepared starch nanoparticles had a mean size and PDI of 352.8 nm and 0.292, respectively.
32902254	9	0	theme	mass	1708:1711	arg1	plot					1720:1723	the two-dimensional relative mass defect plot	1679:1723	the two-dimensional relative mass defect plot	1679:1723	We show that adding the retention time as a third dimension improves the resolution power of the two-dimensional relative mass defect plot and offers the possibility for a more detailed substance class mapping.
32902254	7	1	theme	defect	1301:1306	arg1	plots					1308:1312	the relative mass defect plots	1283:1312	the relative mass defect plots	1283:1312	Using the Khachyan algorithm, elliptical areas clustering substance classes within the relative mass defect plots were calculated.
32902254	4	2	theme	relative	623:630	arg1	plots					644:648	relative mass defect plots	623:648	relative mass defect plots	623:648	We present a fast approach for the initial characterization and substance class profiling using relative mass defect plots for the visualization of herbal compositions.
32902254	1	3	theme	plant	258:262	arg1	classes					275:281	secondary plant metabolite classes	248:281	secondary plant metabolite classes	248:281	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation is intimately connected to fast chemical profiling and visualization of secondary plant metabolite classes or single compounds.
32902254	9	4	theme	plot	1720:1723	arg1	power					1670:1674	the resolution power	1655:1674	the resolution power of the two-dimensional relative mass defect plot	1655:1723	We show that adding the retention time as a third dimension improves the resolution power of the two-dimensional relative mass defect plot and offers the possibility for a more detailed substance class mapping.
32902254	4	5	theme	defect	637:642	arg1	plots					644:648	relative mass defect plots	623:648	relative mass defect plots	623:648	We present a fast approach for the initial characterization and substance class profiling using relative mass defect plots for the visualization of herbal compositions.
32902254	1	6	theme	classes	275:281	arg1	visualization					231:243	visualization	231:243	visualization of secondary plant metabolite classes or single compounds	231:301	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation is intimately connected to fast chemical profiling and visualization of secondary plant metabolite classes or single compounds.
32902254	1	6	theme	classes	275:281	arg1	profiling					217:225	fast chemical profiling	203:225	fast chemical profiling	203:225	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation is intimately connected to fast chemical profiling and visualization of secondary plant metabolite classes or single compounds.
32902254	5	7	theme	annotated	850:858	arg1	mixture					768:774	a varied mixture	759:774	a varied mixture	759:774	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	1	8	theme	preparation	164:174	arg1	analysis					128:135	A holistic, nontargeted mass spectrometric analysis	85:135	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation	85:174	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation is intimately connected to fast chemical profiling and visualization of secondary plant metabolite classes or single compounds.
32902254	2	9	theme	spectral	325:332	arg1	data					334:337	High-resolution mass spectral data	304:337	High-resolution mass spectral data	304:337	High-resolution mass spectral data enable a broad variety of analytical possibilities.
32902254	4	10	theme	herbal	675:680	arg1	compositions					682:693	herbal compositions	675:693	herbal compositions	675:693	We present a fast approach for the initial characterization and substance class profiling using relative mass defect plots for the visualization of herbal compositions.
32902254	2	11	theme	High-resolution	304:318	arg1	data					334:337	High-resolution mass spectral data	304:337	High-resolution mass spectral data	304:337	High-resolution mass spectral data enable a broad variety of analytical possibilities.
32902254	6	12	theme	defect	1002:1007	arg1	calculation					969:979	the calculation	965:979	the calculation of the relative mass defect	965:1007	For the calculation of the relative mass defect, a new approach incorporating two correction functions to obtain correct relative mass defect results also for large hydrocarbons, and a multitude of polyhalogenated molecules was developed.
32902254	9	13	theme	detailed	1763:1770	arg1	mapping					1788:1794	a more detailed substance class mapping	1756:1794	a more detailed substance class mapping	1756:1794	We show that adding the retention time as a third dimension improves the resolution power of the two-dimensional relative mass defect plot and offers the possibility for a more detailed substance class mapping.
32902254	8	14	theme	novel	1345:1349	arg1	fingerprint					1524:1534	a unique fingerprint	1515:1534	a unique fingerprint for herbals, part of them or herbal preparations	1515:1583	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	8	14	theme	novel	1345:1349	arg1	plots					1372:1376	The resulting novel relative mass defect plots	1331:1376	The resulting novel relative mass defect plots	1331:1376	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	5	15	theme	varied	761:766	arg1	mixture					768:774	a varied mixture	759:774	a varied mixture	759:774	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	5	16	from	mixture	768:774	arg1	alkylation					829:838	alkylation	829:838	alkylation	829:838	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	5	16	from	mixture	768:774	arg1	polarity					800:807	polarity	800:807	polarity	800:807	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	5	16	from	mixture	768:774	arg1	glycosylation					810:822	glycosylation	810:822	glycosylation	810:822	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	7	17	theme	Khachyan	1210:1217	arg1	algorithm					1219:1227	the Khachyan algorithm	1206:1227	the Khachyan algorithm	1206:1227	Using the Khachyan algorithm, elliptical areas clustering substance classes within the relative mass defect plots were calculated.
32902254	5	18	theme	relative	887:894	arg1	defects					901:907	the relative mass defects	883:907	the relative mass defects	883:907	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	6	19	theme	relative	988:995	arg1	defect					1002:1007	the relative mass defect	984:1007	the relative mass defect	984:1007	For the calculation of the relative mass defect, a new approach incorporating two correction functions to obtain correct relative mass defect results also for large hydrocarbons, and a multitude of polyhalogenated molecules was developed.
32902254	9	20	theme	class	1782:1786	arg1	mapping					1788:1794	a more detailed substance class mapping	1756:1794	a more detailed substance class mapping	1756:1794	We show that adding the retention time as a third dimension improves the resolution power of the two-dimensional relative mass defect plot and offers the possibility for a more detailed substance class mapping.
32902254	8	21	theme	mass	1360:1363	arg1	fingerprint					1524:1534	a unique fingerprint	1515:1534	a unique fingerprint for herbals, part of them or herbal preparations	1515:1583	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	8	21	theme	mass	1360:1363	arg1	plots					1372:1376	The resulting novel relative mass defect plots	1331:1376	The resulting novel relative mass defect plots	1331:1376	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	5	22	theme	common	719:724	arg1	metabolites					732:742	1160 common plant metabolites	714:742	1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes	714:880	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	1	23	theme	mass	109:112	arg1	analysis					128:135	A holistic, nontargeted mass spectrometric analysis	85:135	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation	85:174	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation is intimately connected to fast chemical profiling and visualization of secondary plant metabolite classes or single compounds.
32902254	9	24	theme	third	1630:1634	arg1	time					1620:1623	the retention time	1606:1623	the retention time	1606:1623	We show that adding the retention time as a third dimension improves the resolution power of the two-dimensional relative mass defect plot and offers the possibility for a more detailed substance class mapping.
32902254	9	24	theme	third	1630:1634	arg1	dimension					1636:1644	a third dimension	1628:1644	a third dimension	1628:1644	We show that adding the retention time as a third dimension improves the resolution power of the two-dimensional relative mass defect plot and offers the possibility for a more detailed substance class mapping.
32902254	5	25	theme	metabolites	732:742	arg1	dataset					703:709	a dataset	701:709	a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes	701:880	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	1	26	dep	holistic	87:94	arg1	nontargeted					97:107	nontargeted	97:107	nontargeted	97:107	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation is intimately connected to fast chemical profiling and visualization of secondary plant metabolite classes or single compounds.
32902254	7	27	theme	mass	1296:1299	arg1	plots					1308:1312	the relative mass defect plots	1283:1312	the relative mass defect plots	1283:1312	Using the Khachyan algorithm, elliptical areas clustering substance classes within the relative mass defect plots were calculated.
32902254	5	28	from	alkylation	829:838	arg1	mixture					768:774	a varied mixture	759:774	a varied mixture	759:774	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	5	29	theme	theoretical	931:941	arg1	masses					953:958	theoretical molecular masses	931:958	theoretical molecular masses	931:958	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	8	30	theme	them	1557:1560	arg1	preparations					1572:1583	herbal preparations	1565:1583	herbal preparations	1565:1583	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	8	30	theme	them	1557:1560	arg1	part					1549:1552	part	1549:1552	part of them	1549:1560	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	8	30	theme	them	1557:1560	arg1	herbals					1540:1546	herbals	1540:1546	herbals	1540:1546	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	0	31	theme	Defect	71:76	arg1	Plots					78:82	Relative Mass Defect Plots	57:82	Relative Mass Defect Plots	57:82	Fast Evaluation of Herbal Substance Class Composition by Relative Mass Defect Plots.
32902254	1	32	theme	material	151:158	arg1	analysis					128:135	A holistic, nontargeted mass spectrometric analysis	85:135	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation	85:174	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation is intimately connected to fast chemical profiling and visualization of secondary plant metabolite classes or single compounds.
32902254	8	33	theme	herbal	1565:1570	arg1	preparations					1572:1583	herbal preparations	1565:1583	herbal preparations	1565:1583	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	8	34	theme	two-dimensional	1401:1415	arg1	mapping					1433:1439	two-dimensional substance class mapping	1401:1439	two-dimensional substance class mapping	1401:1439	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	8	35	theme	class	1427:1431	arg1	mapping					1433:1439	two-dimensional substance class mapping	1401:1439	two-dimensional substance class mapping	1401:1439	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	3	36	theme	phytochemical	452:464	arg1	classes					443:449	compound classes	434:449	compound classes (phytochemical profiling)	434:475	Often a fast and comprehensive overview on compound classes (phytochemical profiling) is needed before single-substance considerations.
32902254	3	36	theme	phytochemical	452:464	arg1	profiling					466:474	phytochemical profiling	452:474	phytochemical profiling	452:474	Often a fast and comprehensive overview on compound classes (phytochemical profiling) is needed before single-substance considerations.
32902254	5	37	theme	classes	789:795	arg1	mixture					768:774	a varied mixture	759:774	a varied mixture	759:774	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	6	38	theme	large	1120:1124	arg1	hydrocarbons					1126:1137	large hydrocarbons	1120:1137	large hydrocarbons	1120:1137	For the calculation of the relative mass defect, a new approach incorporating two correction functions to obtain correct relative mass defect results also for large hydrocarbons, and a multitude of polyhalogenated molecules was developed.
32902254	3	39	theme	fast	399:402	arg1	overview					422:429	a fast and comprehensive overview	397:429	a fast and comprehensive overview on compound classes (phytochemical profiling)	397:475	Often a fast and comprehensive overview on compound classes (phytochemical profiling) is needed before single-substance considerations.
32902254	0	40	theme	Mass	66:69	arg1	Plots					78:82	Relative Mass Defect Plots	57:82	Relative Mass Defect Plots	57:82	Fast Evaluation of Herbal Substance Class Composition by Relative Mass Defect Plots.
32902254	7	41	theme	elliptical	1230:1239	arg1	areas					1241:1245	elliptical areas	1230:1245	elliptical areas clustering substance classes within the relative mass defect plots	1230:1312	Using the Khachyan algorithm, elliptical areas clustering substance classes within the relative mass defect plots were calculated.
32902254	8	42	theme	high-resolution	1455:1469	arg1	data					1485:1488	high-resolution mass spectral data	1455:1488	high-resolution mass spectral data	1455:1488	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	5	43	theme	substance	864:872	arg1	classes					874:880	substance classes	864:880	substance classes	864:880	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	1	44	theme	compounds	293:301	arg1	visualization					231:243	visualization	231:243	visualization of secondary plant metabolite classes or single compounds	231:301	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation is intimately connected to fast chemical profiling and visualization of secondary plant metabolite classes or single compounds.
32902254	1	44	theme	compounds	293:301	arg1	profiling					217:225	fast chemical profiling	203:225	fast chemical profiling	203:225	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation is intimately connected to fast chemical profiling and visualization of secondary plant metabolite classes or single compounds.
32902254	6	45	theme	defect	1096:1101	arg1	results					1103:1109	correct relative mass defect results	1074:1109	correct relative mass defect results	1074:1109	For the calculation of the relative mass defect, a new approach incorporating two correction functions to obtain correct relative mass defect results also for large hydrocarbons, and a multitude of polyhalogenated molecules was developed.
32902254	4	46	theme	class	601:605	arg1	profiling					607:615	substance class profiling	591:615	substance class profiling	591:615	We present a fast approach for the initial characterization and substance class profiling using relative mass defect plots for the visualization of herbal compositions.
32902254	8	47	theme	spectral	1476:1483	arg1	data					1485:1488	high-resolution mass spectral data	1455:1488	high-resolution mass spectral data	1455:1488	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	0	48	theme	Substance	26:34	arg1	Composition					42:52	Herbal Substance Class Composition	19:52	Herbal Substance Class Composition	19:52	Fast Evaluation of Herbal Substance Class Composition by Relative Mass Defect Plots.
32902254	6	49	theme	relative	1082:1089	arg1	results					1103:1109	correct relative mass defect results	1074:1109	correct relative mass defect results	1074:1109	For the calculation of the relative mass defect, a new approach incorporating two correction functions to obtain correct relative mass defect results also for large hydrocarbons, and a multitude of polyhalogenated molecules was developed.
32902254	3	50	theme	comprehensive	408:420	arg1	overview					422:429	a fast and comprehensive overview	397:429	a fast and comprehensive overview on compound classes (phytochemical profiling)	397:475	Often a fast and comprehensive overview on compound classes (phytochemical profiling) is needed before single-substance considerations.
32902254	0	51	theme	Relative	57:64	arg1	Plots					78:82	Relative Mass Defect Plots	57:82	Relative Mass Defect Plots	57:82	Fast Evaluation of Herbal Substance Class Composition by Relative Mass Defect Plots.
32902254	0	52	theme	Composition	42:52	arg1	Evaluation					5:14	Fast Evaluation	0:14	Fast Evaluation of Herbal Substance Class Composition by Relative Mass Defect Plots.	0:83	Fast Evaluation of Herbal Substance Class Composition by Relative Mass Defect Plots.
32902254	4	53	dep	characterization	570:585	arg1	the					558:560	the	558:560	the	558:560	We present a fast approach for the initial characterization and substance class profiling using relative mass defect plots for the visualization of herbal compositions.
32902254	6	54	theme	molecules	1175:1183	arg1	approach					1016:1023	a new approach	1010:1023	a new approach incorporating two correction functions to obtain correct relative mass defect results also for large hydrocarbons	1010:1137	For the calculation of the relative mass defect, a new approach incorporating two correction functions to obtain correct relative mass defect results also for large hydrocarbons, and a multitude of polyhalogenated molecules was developed.
32902254	6	54	theme	molecules	1175:1183	arg1	multitude					1146:1154	a multitude	1144:1154	a multitude of polyhalogenated molecules	1144:1183	For the calculation of the relative mass defect, a new approach incorporating two correction functions to obtain correct relative mass defect results also for large hydrocarbons, and a multitude of polyhalogenated molecules was developed.
32902254	4	55	theme	initial	562:568	arg1	characterization					570:585	initial characterization	562:585	initial characterization	562:585	We present a fast approach for the initial characterization and substance class profiling using relative mass defect plots for the visualization of herbal compositions.
32902254	3	56	theme	single-substance	494:509	arg1	considerations					511:524	single-substance considerations	494:524	single-substance considerations	494:524	Often a fast and comprehensive overview on compound classes (phytochemical profiling) is needed before single-substance considerations.
32902254	9	57	theme	relative	1699:1706	arg1	plot					1720:1723	the two-dimensional relative mass defect plot	1679:1723	the two-dimensional relative mass defect plot	1679:1723	We show that adding the retention time as a third dimension improves the resolution power of the two-dimensional relative mass defect plot and offers the possibility for a more detailed substance class mapping.
32902254	2	58	theme	analytical	365:374	arg1	possibilities					376:388	analytical possibilities	365:388	analytical possibilities	365:388	High-resolution mass spectral data enable a broad variety of analytical possibilities.
32902254	1	59	theme	secondary	248:256	arg1	classes					275:281	secondary plant metabolite classes	248:281	secondary plant metabolite classes	248:281	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation is intimately connected to fast chemical profiling and visualization of secondary plant metabolite classes or single compounds.
32902254	9	60	theme	defect	1713:1718	arg1	plot					1720:1723	the two-dimensional relative mass defect plot	1679:1723	the two-dimensional relative mass defect plot	1679:1723	We show that adding the retention time as a third dimension improves the resolution power of the two-dimensional relative mass defect plot and offers the possibility for a more detailed substance class mapping.
32902254	1	61	theme	metabolite	264:273	arg1	classes					275:281	secondary plant metabolite classes	248:281	secondary plant metabolite classes	248:281	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation is intimately connected to fast chemical profiling and visualization of secondary plant metabolite classes or single compounds.
32902254	5	62	theme	molecular	943:951	arg1	masses					953:958	theoretical molecular masses	931:958	theoretical molecular masses	931:958	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	4	63	theme	mass	632:635	arg1	plots					644:648	relative mass defect plots	623:648	relative mass defect plots	623:648	We present a fast approach for the initial characterization and substance class profiling using relative mass defect plots for the visualization of herbal compositions.
32902254	5	64	theme	mass	896:899	arg1	defects					901:907	the relative mass defects	883:907	the relative mass defects	883:907	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	6	65	theme	new	1012:1014	arg1	approach					1016:1023	a new approach	1010:1023	a new approach incorporating two correction functions to obtain correct relative mass defect results also for large hydrocarbons	1010:1137	For the calculation of the relative mass defect, a new approach incorporating two correction functions to obtain correct relative mass defect results also for large hydrocarbons, and a multitude of polyhalogenated molecules was developed.
32902254	2	66	theme	mass	320:323	arg1	data					334:337	High-resolution mass spectral data	304:337	High-resolution mass spectral data	304:337	High-resolution mass spectral data enable a broad variety of analytical possibilities.
32902254	8	67	theme	unique	1517:1522	arg1	fingerprint					1524:1534	a unique fingerprint	1515:1534	a unique fingerprint for herbals, part of them or herbal preparations	1515:1583	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	8	67	theme	unique	1517:1522	arg1	plots					1372:1376	The resulting novel relative mass defect plots	1331:1376	The resulting novel relative mass defect plots	1331:1376	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	5	68	from	classes	789:795	arg1	alkylation					829:838	alkylation	829:838	alkylation	829:838	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	5	68	from	classes	789:795	arg1	polarity					800:807	polarity	800:807	polarity	800:807	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	5	68	from	classes	789:795	arg1	glycosylation					810:822	glycosylation	810:822	glycosylation	810:822	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	1	69	theme	fast	203:206	arg1	profiling					217:225	fast chemical profiling	203:225	fast chemical profiling	203:225	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation is intimately connected to fast chemical profiling and visualization of secondary plant metabolite classes or single compounds.
32902254	6	70	theme	mass	997:1000	arg1	defect					1002:1007	the relative mass defect	984:1007	the relative mass defect	984:1007	For the calculation of the relative mass defect, a new approach incorporating two correction functions to obtain correct relative mass defect results also for large hydrocarbons, and a multitude of polyhalogenated molecules was developed.
32902254	9	71	theme	substance	1772:1780	arg1	mapping					1788:1794	a more detailed substance class mapping	1756:1794	a more detailed substance class mapping	1756:1794	We show that adding the retention time as a third dimension improves the resolution power of the two-dimensional relative mass defect plot and offers the possibility for a more detailed substance class mapping.
32902254	4	72	theme	compositions	682:693	arg1	visualization					658:670	the visualization	654:670	the visualization of herbal compositions	654:693	We present a fast approach for the initial characterization and substance class profiling using relative mass defect plots for the visualization of herbal compositions.
32902254	4	73	theme	fast	540:543	arg1	approach					545:552	a fast approach	538:552	a fast approach for the initial characterization and substance class profiling using relative mass defect plots for the visualization of herbal compositions	538:693	We present a fast approach for the initial characterization and substance class profiling using relative mass defect plots for the visualization of herbal compositions.
32902254	8	74	theme	relative	1351:1358	arg1	fingerprint					1524:1534	a unique fingerprint	1515:1534	a unique fingerprint for herbals, part of them or herbal preparations	1515:1583	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	8	74	theme	relative	1351:1358	arg1	plots					1372:1376	The resulting novel relative mass defect plots	1331:1376	The resulting novel relative mass defect plots	1331:1376	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	5	75	theme	plant	726:730	arg1	metabolites					732:742	1160 common plant metabolites	714:742	1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes	714:880	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	5	76	from	glycosylation	810:822	arg1	mixture					768:774	a varied mixture	759:774	a varied mixture	759:774	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	1	77	theme	spectrometric	114:126	arg1	analysis					128:135	A holistic, nontargeted mass spectrometric analysis	85:135	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation	85:174	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation is intimately connected to fast chemical profiling and visualization of secondary plant metabolite classes or single compounds.
32902254	6	78	theme	correct	1074:1080	arg1	results					1103:1109	correct relative mass defect results	1074:1109	correct relative mass defect results	1074:1109	For the calculation of the relative mass defect, a new approach incorporating two correction functions to obtain correct relative mass defect results also for large hydrocarbons, and a multitude of polyhalogenated molecules was developed.
32902254	8	79	theme	defect	1365:1370	arg1	fingerprint					1524:1534	a unique fingerprint	1515:1534	a unique fingerprint for herbals, part of them or herbal preparations	1515:1583	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	8	79	theme	defect	1365:1370	arg1	plots					1372:1376	The resulting novel relative mass defect plots	1331:1376	The resulting novel relative mass defect plots	1331:1376	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	7	80	theme	relative	1287:1294	arg1	plots					1308:1312	the relative mass defect plots	1283:1312	the relative mass defect plots	1283:1312	Using the Khachyan algorithm, elliptical areas clustering substance classes within the relative mass defect plots were calculated.
32902254	5	81	from	polarity	800:807	arg1	mixture					768:774	a varied mixture	759:774	a varied mixture	759:774	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	3	82	from	overview	422:429	arg1	classes					443:449	compound classes	434:449	compound classes (phytochemical profiling)	434:475	Often a fast and comprehensive overview on compound classes (phytochemical profiling) is needed before single-substance considerations.
32902254	3	82	from	overview	422:429	arg1	profiling					466:474	phytochemical profiling	452:474	phytochemical profiling	452:474	Often a fast and comprehensive overview on compound classes (phytochemical profiling) is needed before single-substance considerations.
32902254	1	83	theme	herbal	144:149	arg1	material					151:158	any herbal material	140:158	any herbal material	140:158	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation is intimately connected to fast chemical profiling and visualization of secondary plant metabolite classes or single compounds.
32902254	6	84	theme	correction	1043:1052	arg1	functions					1054:1062	two correction functions	1039:1062	two correction functions	1039:1062	For the calculation of the relative mass defect, a new approach incorporating two correction functions to obtain correct relative mass defect results also for large hydrocarbons, and a multitude of polyhalogenated molecules was developed.
32902254	8	85	theme	quick	1388:1392	arg1	way					1394:1396	a quick way	1386:1396	a quick way of two-dimensional substance class mapping	1386:1439	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	7	86	theme	substance	1258:1266	arg1	classes					1268:1274	substance classes	1258:1274	substance classes within the relative mass defect plots	1258:1312	Using the Khachyan algorithm, elliptical areas clustering substance classes within the relative mass defect plots were calculated.
32902254	0	87	theme	Fast	0:3	arg1	Evaluation					5:14	Fast Evaluation	0:14	Fast Evaluation of Herbal Substance Class Composition by Relative Mass Defect Plots.	0:83	Fast Evaluation of Herbal Substance Class Composition by Relative Mass Defect Plots.
32902254	5	88	theme	molecular	779:787	arg1	classes					789:795	molecular classes	779:795	molecular classes in polarity, glycosylation, and alkylation	779:838	From a dataset of 1160 common plant metabolites that represent a varied mixture of molecular classes in polarity, glycosylation, and alkylation, manually annotated for substance classes, the relative mass defects were calculated using theoretical molecular masses.
32902254	9	89	theme	retention	1610:1618	arg1	time					1620:1623	the retention time	1606:1623	the retention time	1606:1623	We show that adding the retention time as a third dimension improves the resolution power of the two-dimensional relative mass defect plot and offers the possibility for a more detailed substance class mapping.
32902254	9	89	theme	retention	1610:1618	arg1	dimension					1636:1644	a third dimension	1628:1644	a third dimension	1628:1644	We show that adding the retention time as a third dimension improves the resolution power of the two-dimensional relative mass defect plot and offers the possibility for a more detailed substance class mapping.
32902254	8	90	theme	substance	1417:1425	arg1	mapping					1433:1439	two-dimensional substance class mapping	1401:1439	two-dimensional substance class mapping	1401:1439	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	4	91	theme	substance	591:599	arg1	profiling					607:615	substance class profiling	591:615	substance class profiling	591:615	We present a fast approach for the initial characterization and substance class profiling using relative mass defect plots for the visualization of herbal compositions.
32902254	8	92	theme	mapping	1433:1439	arg1	way					1394:1396	a quick way	1386:1396	a quick way of two-dimensional substance class mapping	1386:1439	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	0	93	theme	Herbal	19:24	arg1	Composition					42:52	Herbal Substance Class Composition	19:52	Herbal Substance Class Composition	19:52	Fast Evaluation of Herbal Substance Class Composition by Relative Mass Defect Plots.
32902254	1	94	theme	single	286:291	arg1	compounds					293:301	single compounds	286:301	single compounds	286:301	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation is intimately connected to fast chemical profiling and visualization of secondary plant metabolite classes or single compounds.
32902254	1	95	theme	holistic	87:94	arg1	analysis					128:135	A holistic, nontargeted mass spectrometric analysis	85:135	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation	85:174	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation is intimately connected to fast chemical profiling and visualization of secondary plant metabolite classes or single compounds.
32902254	0	96	theme	Class	36:40	arg1	Composition					42:52	Herbal Substance Class Composition	19:52	Herbal Substance Class Composition	19:52	Fast Evaluation of Herbal Substance Class Composition by Relative Mass Defect Plots.
32902254	6	97	theme	mass	1091:1094	arg1	results					1103:1109	correct relative mass defect results	1074:1109	correct relative mass defect results	1074:1109	For the calculation of the relative mass defect, a new approach incorporating two correction functions to obtain correct relative mass defect results also for large hydrocarbons, and a multitude of polyhalogenated molecules was developed.
32902254	3	98	theme	compound	434:441	arg1	classes					443:449	compound classes	434:449	compound classes (phytochemical profiling)	434:475	Often a fast and comprehensive overview on compound classes (phytochemical profiling) is needed before single-substance considerations.
32902254	3	98	theme	compound	434:441	arg1	profiling					466:474	phytochemical profiling	452:474	phytochemical profiling	452:474	Often a fast and comprehensive overview on compound classes (phytochemical profiling) is needed before single-substance considerations.
32902254	8	99	theme	mass	1471:1474	arg1	data					1485:1488	high-resolution mass spectral data	1455:1488	high-resolution mass spectral data	1455:1488	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	9	100	theme	resolution	1659:1668	arg1	power					1670:1674	the resolution power	1655:1674	the resolution power of the two-dimensional relative mass defect plot	1655:1723	We show that adding the retention time as a third dimension improves the resolution power of the two-dimensional relative mass defect plot and offers the possibility for a more detailed substance class mapping.
32902254	2	101	theme	broad	348:352	arg1	variety					354:360	a broad variety	346:360	a broad variety of analytical possibilities	346:388	High-resolution mass spectral data enable a broad variety of analytical possibilities.
32902254	2	101	theme	broad	348:352	arg1	possibilities					376:388	analytical possibilities	365:388	analytical possibilities	365:388	High-resolution mass spectral data enable a broad variety of analytical possibilities.
32902254	6	102	theme	polyhalogenated	1159:1173	arg1	molecules					1175:1183	polyhalogenated molecules	1159:1183	polyhalogenated molecules	1159:1183	For the calculation of the relative mass defect, a new approach incorporating two correction functions to obtain correct relative mass defect results also for large hydrocarbons, and a multitude of polyhalogenated molecules was developed.
32902254	8	103	theme	resulting	1335:1343	arg1	fingerprint					1524:1534	a unique fingerprint	1515:1534	a unique fingerprint for herbals, part of them or herbal preparations	1515:1583	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	8	103	theme	resulting	1335:1343	arg1	plots					1372:1376	The resulting novel relative mass defect plots	1331:1376	The resulting novel relative mass defect plots	1331:1376	The resulting novel relative mass defect plots provide a quick way of two-dimensional substance class mapping directly from high-resolution mass spectral data and may be considered as a unique fingerprint for herbals, part of them or herbal preparations.
32902254	1	104	theme	chemical	208:215	arg1	profiling					217:225	fast chemical profiling	203:225	fast chemical profiling	203:225	A holistic, nontargeted mass spectrometric analysis of any herbal material and preparation is intimately connected to fast chemical profiling and visualization of secondary plant metabolite classes or single compounds.
32902254	9	105	theme	two-dimensional	1683:1697	arg1	plot					1720:1723	the two-dimensional relative mass defect plot	1679:1723	the two-dimensional relative mass defect plot	1679:1723	We show that adding the retention time as a third dimension improves the resolution power of the two-dimensional relative mass defect plot and offers the possibility for a more detailed substance class mapping.
32902254	2	106	theme	possibilities	376:388	arg1	variety					354:360	a broad variety	346:360	a broad variety of analytical possibilities	346:388	High-resolution mass spectral data enable a broad variety of analytical possibilities.
32902254	2	106	theme	possibilities	376:388	arg1	possibilities					376:388	analytical possibilities	365:388	analytical possibilities	365:388	High-resolution mass spectral data enable a broad variety of analytical possibilities.
34439756	5	0	theme	dismutase	1141:1149	arg1	activity					1151:1158	increased catalase and superoxide dismutase activity	1107:1158	activity	1151:1158	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	3	1	theme	various	484:490	arg1	methods					504:510	various biochemical methods	484:510	various biochemical methods for determination of the nutrient composition	484:556	Intracellular fractions from fungi grown on a medium supplemented with cow milk were analyzed using various biochemical methods for determination of the nutrient composition.
34439756	5	2	theme	catalase	1182:1189	arg1	activity					1191:1198	catalase activity	1182:1198	catalase activity	1182:1198	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	5	3	dep	increase	1170:1177	arg1	7.5-fold					1161:1168	7.5-fold	1161:1168	7.5-fold	1161:1168	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	6	4	theme	cancer	1295:1300	arg1	cells					1302:1306	the cancer cells	1291:1306	the cancer cells	1291:1306	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	6	5	theme	2-fold	1547:1552	arg1	effects					1532:1538	pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects	1423:1538	pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts	1423:1578	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	6	5	theme	2-fold	1547:1552	arg1	increase					1554:1561	about 2-fold increase	1541:1561	about 2-fold increase	1541:1561	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	7	6	theme	biomolecules	1701:1712	arg1	source					1683:1688	a source	1681:1688	a source of healthy biomolecules in modern medicine or functional foods	1681:1751	This study suggests that PSC has multiple nutritional and anti-cancer properties and can be used as a source of healthy biomolecules in modern medicine or functional foods.
34439756	7	6	theme	biomolecules	1701:1712	arg1	PSC					1606:1608	PSC	1606:1608	PSC	1606:1608	This study suggests that PSC has multiple nutritional and anti-cancer properties and can be used as a source of healthy biomolecules in modern medicine or functional foods.
34439756	3	7	theme	biochemical	492:502	arg1	methods					504:510	various biochemical methods	484:510	various biochemical methods for determination of the nutrient composition	484:556	Intracellular fractions from fungi grown on a medium supplemented with cow milk were analyzed using various biochemical methods for determination of the nutrient composition.
34439756	4	8	theme	anti-cancer	572:582	arg1	properties					584:593	anti-cancer properties	572:593	anti-cancer properties of selected extracts	572:614	Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro.
34439756	5	9	theme	Biochemical	703:713	arg1	analysis					715:722	Biochemical analysis	703:722	Biochemical analysis	703:722	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	7	10	from	source	1683:1688	arg1	medicine					1724:1731	modern medicine	1717:1731	modern medicine	1717:1731	This study suggests that PSC has multiple nutritional and anti-cancer properties and can be used as a source of healthy biomolecules in modern medicine or functional foods.
34439756	7	10	from	source	1683:1688	arg1	foods					1747:1751	functional foods	1736:1751	functional foods	1736:1751	This study suggests that PSC has multiple nutritional and anti-cancer properties and can be used as a source of healthy biomolecules in modern medicine or functional foods.
34439756	6	11	from	control	1362:1368	arg1	cells					1390:1394	HT-29 and SW948 cells	1374:1394	cells	1390:1394	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	6	12	from	cells	1390:1394	arg1	control					1362:1368	the control	1358:1368	the control	1358:1368	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	6	12	from	cells	1390:1394	arg1	%					1353:1353	40.0 ± 8.6%	1343:1353	40.0 ± 8.6% of the control, on HT-29 and SW948 cells	1343:1394	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	6	12	from	cells	1390:1394	arg1	%					1337:1337	60.0 ± 6.8%	1327:1337	60.0 ± 6.8%	1327:1337	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	6	13	theme	SW948	1384:1388	arg1	cells					1390:1394	HT-29 and SW948 cells	1374:1394	cells	1390:1394	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	1	14	theme	pro-health	239:248	arg1	substances					250:259	pro-health substances	239:259	pro-health substances	239:259	The present study aimed to demonstrate Lentinus (formerly Pleurotus) sajor-caju (PSC) as a good source of pro-health substances.
34439756	7	15	theme	multiple	1614:1621	arg1	properties					1651:1660	multiple nutritional and anti-cancer properties	1614:1660	multiple nutritional and anti-cancer properties	1614:1660	This study suggests that PSC has multiple nutritional and anti-cancer properties and can be used as a source of healthy biomolecules in modern medicine or functional foods.
34439756	7	16	theme	functional	1736:1745	arg1	foods					1747:1751	functional foods	1736:1751	functional foods	1736:1751	This study suggests that PSC has multiple nutritional and anti-cancer properties and can be used as a source of healthy biomolecules in modern medicine or functional foods.
34439756	3	17	theme	nutrient	537:544	arg1	composition					546:556	the nutrient composition	533:556	the nutrient composition	533:556	Intracellular fractions from fungi grown on a medium supplemented with cow milk were analyzed using various biochemical methods for determination of the nutrient composition.
34439756	1	18	theme	substances	250:259	arg1	sajor-caju					202:211	Lentinus (formerly Pleurotus) sajor-caju	172:211	Lentinus (formerly Pleurotus) sajor-caju (PSC)	172:217	The present study aimed to demonstrate Lentinus (formerly Pleurotus) sajor-caju (PSC) as a good source of pro-health substances.
34439756	1	18	theme	substances	250:259	arg1	source					229:234	a good source	222:234	a good source of pro-health substances	222:259	The present study aimed to demonstrate Lentinus (formerly Pleurotus) sajor-caju (PSC) as a good source of pro-health substances.
34439756	5	19	theme	medium	1053:1058	arg1	mixture					1060:1066	medium mixture	1053:1066	medium mixture (1:1) (PSC3-II)	1053:1082	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	5	19	theme	medium	1053:1058	arg1	1:1					1069:1071	1:1	1069:1071	1:1	1069:1071	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	5	19	theme	medium	1053:1058	arg1	PSC3-II					1075:1081	PSC3-II	1075:1081	PSC3-II	1075:1081	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	7	20	theme	modern	1717:1722	arg1	medicine					1724:1731	modern medicine	1717:1731	modern medicine	1717:1731	This study suggests that PSC has multiple nutritional and anti-cancer properties and can be used as a source of healthy biomolecules in modern medicine or functional foods.
34439756	4	21	theme	colorectal	637:646	arg1	HT-29					667:671	HT-29	667:671	HT-29	667:671	Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro.
34439756	4	21	theme	colorectal	637:646	arg1	lines					660:664	colorectal cancer cell lines	637:664	colorectal cancer cell lines (HT-29, LS 180, and SW948)	637:691	Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro.
34439756	4	21	theme	colorectal	637:646	arg1	SW948					686:690	SW948	686:690	SW948	686:690	Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro.
34439756	4	21	theme	colorectal	637:646	arg1	LS					674:675	LS 180	674:679	LS 180	674:679	Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro.
34439756	5	22	theme	proteins	863:870	arg1	concentration					846:858	concentration	846:858	concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I)	846:942	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	1	23	theme	Lentinus	172:179	arg1	sajor-caju					202:211	Lentinus (formerly Pleurotus) sajor-caju	172:211	Lentinus (formerly Pleurotus) sajor-caju (PSC)	172:217	The present study aimed to demonstrate Lentinus (formerly Pleurotus) sajor-caju (PSC) as a good source of pro-health substances.
34439756	1	23	theme	Lentinus	172:179	arg1	source					229:234	a good source	222:234	a good source of pro-health substances	222:259	The present study aimed to demonstrate Lentinus (formerly Pleurotus) sajor-caju (PSC) as a good source of pro-health substances.
34439756	1	23	theme	Lentinus	172:179	arg1	PSC					214:216	PSC	214:216	PSC	214:216	The present study aimed to demonstrate Lentinus (formerly Pleurotus) sajor-caju (PSC) as a good source of pro-health substances.
34439756	0	24	theme	Pleurotus	110:118	arg1	Cultures					88:95	Milk-Supplemented Cultures	70:95	Milk-Supplemented Cultures of Lentinus (Pleurotus) Sajor-caju	70:130	Pro-Health and Anti-Cancer Activity of Fungal Fractions Isolated from Milk-Supplemented Cultures of Lentinus (Pleurotus) Sajor-caju.
34439756	5	25	theme	mycelia	904:910	arg1	proteins					863:870	proteins	863:870	proteins	863:870	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	5	25	theme	mycelia	904:910	arg1	extracts					892:899	carbohydratesin extracts	876:899	carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I)	876:942	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	2	26	theme	beneficial	361:370	arg1	properties					372:381	its beneficial properties	357:381	its beneficial properties	357:381	It has also shown that supplementation of its culture medium with cow milk may further improve its beneficial properties.
34439756	5	27	from	decrease	967:974	arg1	level					983:987	the level	979:987	the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II))	979:1083	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	6	28	theme	±	1348:1348	arg1	%					1353:1353	40.0 ± 8.6%	1343:1353	40.0 ± 8.6% of the control, on HT-29 and SW948 cells	1343:1394	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	6	28	theme	±	1348:1348	arg1	control					1362:1368	the control	1358:1368	the control	1358:1368	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	5	29	from	decrease	1015:1022	arg1	extract					1027:1033	extract	1027:1033	extract grown on milk and medium mixture (1:1) (PSC3-II)	1027:1082	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	3	30	theme	Intracellular	384:396	arg1	fractions					398:406	Intracellular fractions	384:406	Intracellular fractions from fungi grown on a medium supplemented with cow milk	384:462	Intracellular fractions from fungi grown on a medium supplemented with cow milk were analyzed using various biochemical methods for determination of the nutrient composition.
34439756	5	31	from	3.5-	816:819	arg1	concentration					846:858	concentration	846:858	concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I)	846:942	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	1	32	theme	formerly	182:189	arg1	sajor-caju					202:211	Lentinus (formerly Pleurotus) sajor-caju	172:211	Lentinus (formerly Pleurotus) sajor-caju (PSC)	172:217	The present study aimed to demonstrate Lentinus (formerly Pleurotus) sajor-caju (PSC) as a good source of pro-health substances.
34439756	1	32	theme	formerly	182:189	arg1	source					229:234	a good source	222:234	a good source of pro-health substances	222:259	The present study aimed to demonstrate Lentinus (formerly Pleurotus) sajor-caju (PSC) as a good source of pro-health substances.
34439756	1	32	theme	formerly	182:189	arg1	PSC					214:216	PSC	214:216	PSC	214:216	The present study aimed to demonstrate Lentinus (formerly Pleurotus) sajor-caju (PSC) as a good source of pro-health substances.
34439756	0	33	theme	Pro-Health	0:9	arg1	Activity					27:34	Pro-Health and Anti-Cancer Activity	0:34	Pro-Health and Anti-Cancer Activity of Fungal Fractions	0:54	Pro-Health and Anti-Cancer Activity of Fungal Fractions Isolated from Milk-Supplemented Cultures of Lentinus (Pleurotus) Sajor-caju.
34439756	7	34	used	used	1673:1676	arg2	PSC					1606:1608	PSC	1606:1608	PSC	1606:1608	This study suggests that PSC has multiple nutritional and anti-cancer properties and can be used as a source of healthy biomolecules in modern medicine or functional foods.
34439756	7	34	used	used	1673:1676	arg2	source					1683:1688	a source	1681:1688	a source of healthy biomolecules in modern medicine or functional foods	1681:1751	This study suggests that PSC has multiple nutritional and anti-cancer properties and can be used as a source of healthy biomolecules in modern medicine or functional foods.
34439756	5	35	from	activity	1218:1225	arg1	PSC3-II					1230:1236	PSC3-II	1230:1236	PSC3-II compared to the control	1230:1260	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	1	36	theme	Pleurotus	191:199	arg1	sajor-caju					202:211	Lentinus (formerly Pleurotus) sajor-caju	172:211	Lentinus (formerly Pleurotus) sajor-caju (PSC)	172:217	The present study aimed to demonstrate Lentinus (formerly Pleurotus) sajor-caju (PSC) as a good source of pro-health substances.
34439756	1	36	theme	Pleurotus	191:199	arg1	source					229:234	a good source	222:234	a good source of pro-health substances	222:259	The present study aimed to demonstrate Lentinus (formerly Pleurotus) sajor-caju (PSC) as a good source of pro-health substances.
34439756	1	36	theme	Pleurotus	191:199	arg1	PSC					214:216	PSC	214:216	PSC	214:216	The present study aimed to demonstrate Lentinus (formerly Pleurotus) sajor-caju (PSC) as a good source of pro-health substances.
34439756	6	37	theme	±	1332:1332	arg1	control					1362:1368	the control	1358:1368	the control	1358:1368	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	6	37	theme	±	1332:1332	arg1	%					1337:1337	60.0 ± 6.8%	1327:1337	60.0 ± 6.8%	1327:1337	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	7	38	contain	has	1610:1612	arg1	source					1683:1688	a source	1681:1688	a source of healthy biomolecules in modern medicine or functional foods	1681:1751	This study suggests that PSC has multiple nutritional and anti-cancer properties and can be used as a source of healthy biomolecules in modern medicine or functional foods.
34439756	7	38	contain	has	1610:1612	arg1	PSC					1606:1608	PSC	1606:1608	PSC	1606:1608	This study suggests that PSC has multiple nutritional and anti-cancer properties and can be used as a source of healthy biomolecules in modern medicine or functional foods.
34439756	7	38	contain	has	1610:1612	arg2	properties					1651:1660	multiple nutritional and anti-cancer properties	1614:1660	multiple nutritional and anti-cancer properties	1614:1660	This study suggests that PSC has multiple nutritional and anti-cancer properties and can be used as a source of healthy biomolecules in modern medicine or functional foods.
34439756	6	39	dep	diminished	1312:1321	arg1	control					1362:1368	the control	1358:1368	the control	1358:1368	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	6	39	dep	diminished	1312:1321	arg1	%					1353:1353	40.0 ± 8.6%	1343:1353	40.0 ± 8.6% of the control, on HT-29 and SW948 cells	1343:1394	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	6	39	dep	diminished	1312:1321	arg1	%					1337:1337	60.0 ± 6.8%	1327:1337	60.0 ± 6.8%	1327:1337	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	6	40	theme	±	1462:1462	arg1	%					1467:1467	18.8 ± 11.8 and 14.7 ± 8.0%	1441:1467	%	1467:1467	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	3	41	theme	composition	546:556	arg1	determination					516:528	determination	516:528	determination of the nutrient composition	516:556	Intracellular fractions from fungi grown on a medium supplemented with cow milk were analyzed using various biochemical methods for determination of the nutrient composition.
34439756	6	42	theme	HT-29	1374:1378	arg1	cells					1390:1394	HT-29 and SW948 cells	1374:1394	cells	1390:1394	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	4	43	theme	cancer	648:653	arg1	HT-29					667:671	HT-29	667:671	HT-29	667:671	Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro.
34439756	4	43	theme	cancer	648:653	arg1	lines					660:664	colorectal cancer cell lines	637:664	colorectal cancer cell lines (HT-29, LS 180, and SW948)	637:691	Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro.
34439756	4	43	theme	cancer	648:653	arg1	SW948					686:690	SW948	686:690	SW948	686:690	Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro.
34439756	4	43	theme	cancer	648:653	arg1	LS					674:675	LS 180	674:679	LS 180	674:679	Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro.
34439756	0	44	theme	Anti-Cancer	15:25	arg1	Activity					27:34	Pro-Health and Anti-Cancer Activity	0:34	Pro-Health and Anti-Cancer Activity of Fungal Fractions	0:54	Pro-Health and Anti-Cancer Activity of Fungal Fractions Isolated from Milk-Supplemented Cultures of Lentinus (Pleurotus) Sajor-caju.
34439756	7	45	theme	healthy	1693:1699	arg1	biomolecules					1701:1712	healthy biomolecules	1693:1712	healthy biomolecules	1693:1712	This study suggests that PSC has multiple nutritional and anti-cancer properties and can be used as a source of healthy biomolecules in modern medicine or functional foods.
34439756	2	46	theme	cow	328:330	arg1	milk					332:335	cow milk	328:335	cow milk	328:335	It has also shown that supplementation of its culture medium with cow milk may further improve its beneficial properties.
34439756	3	47	theme	cow	455:457	arg1	milk					459:462	cow milk	455:462	cow milk	455:462	Intracellular fractions from fungi grown on a medium supplemented with cow milk were analyzed using various biochemical methods for determination of the nutrient composition.
34439756	0	48	theme	Fungal	39:44	arg1	Fractions					46:54	Fungal Fractions	39:54	Fungal Fractions	39:54	Pro-Health and Anti-Cancer Activity of Fungal Fractions Isolated from Milk-Supplemented Cultures of Lentinus (Pleurotus) Sajor-caju.
34439756	5	49	theme	increased	1107:1115	arg1	catalase					1117:1124	increased catalase and superoxide dismutase activity	1107:1158	catalase	1117:1124	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	5	50	from	enrichment	731:740	arg1	proteins					781:788	proteins	781:788	proteins	781:788	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	5	50	from	enrichment	731:740	arg1	compounds					762:770	health-enhancing compounds	745:770	health-enhancing compounds	745:770	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	5	50	from	enrichment	731:740	arg1	polysaccharides					793:807	polysaccharides	793:807	polysaccharides	793:807	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	4	51	dep	lines	660:664	arg1	HT-29					667:671	HT-29	667:671	HT-29	667:671	Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro.
34439756	4	51	dep	lines	660:664	arg1	SW948					686:690	SW948	686:690	SW948	686:690	Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro.
34439756	4	51	dep	lines	660:664	arg1	lines					660:664	colorectal cancer cell lines	637:664	colorectal cancer cell lines (HT-29, LS 180, and SW948)	637:691	Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro.
34439756	4	51	dep	lines	660:664	arg1	LS					674:675	LS 180	674:679	LS 180	674:679	Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro.
34439756	7	52	theme	anti-cancer	1639:1649	arg1	properties					1651:1660	multiple nutritional and anti-cancer properties	1614:1660	multiple nutritional and anti-cancer properties	1614:1660	This study suggests that PSC has multiple nutritional and anti-cancer properties and can be used as a source of healthy biomolecules in modern medicine or functional foods.
34439756	6	53	from	%	1337:1337	arg1	cells					1390:1394	HT-29 and SW948 cells	1374:1394	cells	1390:1394	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	6	54	from	%	1353:1353	arg1	cells					1390:1394	HT-29 and SW948 cells	1374:1394	cells	1390:1394	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	4	55	theme	cell	655:658	arg1	HT-29					667:671	HT-29	667:671	HT-29	667:671	Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro.
34439756	4	55	theme	cell	655:658	arg1	lines					660:664	colorectal cancer cell lines	637:664	colorectal cancer cell lines (HT-29, LS 180, and SW948)	637:691	Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro.
34439756	4	55	theme	cell	655:658	arg1	SW948					686:690	SW948	686:690	SW948	686:690	Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro.
34439756	4	55	theme	cell	655:658	arg1	LS					674:675	LS 180	674:679	LS 180	674:679	Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro.
34439756	2	56	with	supplementation	285:299	arg1	milk					332:335	cow milk	328:335	cow milk	328:335	It has also shown that supplementation of its culture medium with cow milk may further improve its beneficial properties.
34439756	6	57	theme	extracts	1571:1578	arg1	effects					1532:1538	pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects	1423:1538	pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts	1423:1578	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	6	57	theme	extracts	1571:1578	arg1	increase					1554:1561	about 2-fold increase	1541:1561	about 2-fold increase	1541:1561	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	5	58	theme	health-enhancing	745:760	arg1	proteins					781:788	proteins	781:788	proteins	781:788	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	5	58	theme	health-enhancing	745:760	arg1	compounds					762:770	health-enhancing compounds	745:770	health-enhancing compounds	745:770	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	5	58	theme	health-enhancing	745:760	arg1	polysaccharides					793:807	polysaccharides	793:807	polysaccharides	793:807	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	5	59	theme	free	992:995	arg1	radicals					997:1004	free radicals	992:1004	free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II))	992:1083	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	6	60	theme	NO-secreting	1519:1530	arg1	effects					1532:1538	pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects	1423:1538	pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts	1423:1578	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	6	60	theme	NO-secreting	1519:1530	arg1	increase					1554:1561	about 2-fold increase	1541:1561	about 2-fold increase	1541:1561	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	0	61	theme	Fractions	46:54	arg1	Activity					27:34	Pro-Health and Anti-Cancer Activity	0:34	Pro-Health and Anti-Cancer Activity of Fungal Fractions	0:54	Pro-Health and Anti-Cancer Activity of Fungal Fractions Isolated from Milk-Supplemented Cultures of Lentinus (Pleurotus) Sajor-caju.
34439756	6	62	theme	SW948	1488:1492	arg1	cells					1494:1498	LS 180 and SW948 cells	1477:1498	cells	1494:1498	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	5	63	theme	radicals	997:1004	arg1	level					983:987	the level	979:987	the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II))	979:1083	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	2	64	theme	medium	316:321	arg1	supplementation					285:299	supplementation	285:299	supplementation of its culture medium with cow milk	285:335	It has also shown that supplementation of its culture medium with cow milk may further improve its beneficial properties.
34439756	6	65	theme	control	1362:1368	arg1	control					1362:1368	the control	1358:1368	the control	1358:1368	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	6	65	theme	control	1362:1368	arg1	%					1353:1353	40.0 ± 8.6%	1343:1353	40.0 ± 8.6% of the control, on HT-29 and SW948 cells	1343:1394	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	6	65	theme	control	1362:1368	arg1	%					1337:1337	60.0 ± 6.8%	1327:1337	60.0 ± 6.8%	1327:1337	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	5	66	theme	10-fold	1007:1013	arg1	decrease					1015:1022	10-fold decrease	1007:1022	10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)	1007:1082	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	5	67	theme	superoxide	1130:1139	arg1	activity					1151:1158	increased catalase and superoxide dismutase activity	1107:1158	activity	1151:1158	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	6	68	theme	pro-apoptotic	1423:1435	arg1	effects					1532:1538	pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects	1423:1538	pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts	1423:1578	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	6	68	theme	pro-apoptotic	1423:1435	arg1	increase					1554:1561	about 2-fold increase	1541:1561	about 2-fold increase	1541:1561	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	2	69	theme	culture	308:314	arg1	medium					316:321	its culture medium	304:321	its culture medium	304:321	It has also shown that supplementation of its culture medium with cow milk may further improve its beneficial properties.
34439756	5	70	theme	whole	924:928	arg1	PSC2-I					936:941	PSC2-I	936:941	PSC2-I	936:941	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	5	70	theme	whole	924:928	arg1	milk					930:933	whole milk	924:933	whole milk (PSC2-I)	924:942	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	5	71	dep	catalase	1117:1124	arg1	increase					1170:1177	increase	1170:1177	increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control	1170:1260	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	6	72	theme	cells	1302:1306	arg1	viability					1278:1286	the viability	1274:1286	the viability of the cancer cells	1274:1306	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	3	73	from	fungi	413:417	arg1	fractions					398:406	Intracellular fractions	384:406	Intracellular fractions from fungi grown on a medium supplemented with cow milk	384:462	Intracellular fractions from fungi grown on a medium supplemented with cow milk were analyzed using various biochemical methods for determination of the nutrient composition.
34439756	1	74	theme	present	137:143	arg1	study					145:149	The present study	133:149	The present study	133:149	The present study aimed to demonstrate Lentinus (formerly Pleurotus) sajor-caju (PSC) as a good source of pro-health substances.
34439756	5	75	theme	SOD	1214:1216	arg1	activity					1218:1225	SOD activity	1214:1225	SOD activity in PSC3-II compared to the control	1214:1260	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	4	76	theme	extracts	607:614	arg1	properties					584:593	anti-cancer properties	572:593	anti-cancer properties of selected extracts	572:614	Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro.
34439756	0	77	theme	Milk-Supplemented	70:86	arg1	Cultures					88:95	Milk-Supplemented Cultures	70:95	Milk-Supplemented Cultures of Lentinus (Pleurotus) Sajor-caju	70:130	Pro-Health and Anti-Cancer Activity of Fungal Fractions Isolated from Milk-Supplemented Cultures of Lentinus (Pleurotus) Sajor-caju.
34439756	7	78	theme	nutritional	1623:1633	arg1	properties					1651:1660	multiple nutritional and anti-cancer properties	1614:1660	multiple nutritional and anti-cancer properties	1614:1660	This study suggests that PSC has multiple nutritional and anti-cancer properties and can be used as a source of healthy biomolecules in modern medicine or functional foods.
34439756	5	79	theme	carbohydratesin	876:890	arg1	extracts					892:899	carbohydratesin extracts	876:899	carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I)	876:942	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	5	80	dep	radicals	997:1004	arg1	decrease					1015:1022	10-fold decrease	1007:1022	10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)	1007:1082	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	6	81	dep	pro-apoptotic	1423:1435	arg1	±					1446:1446	18.8 ± 11.8 and 14.7 ± 8.0%	1441:1467	±	1446:1446	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	6	81	dep	pro-apoptotic	1423:1435	arg1	%					1467:1467	18.8 ± 11.8 and 14.7 ± 8.0%	1441:1467	%	1467:1467	Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts.
34439756	4	82	theme	selected	598:605	arg1	extracts					607:614	selected extracts	598:614	selected extracts	598:614	Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro.
34439756	5	83	theme	extracts	892:899	arg1	concentration					846:858	concentration	846:858	concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I)	846:942	Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control).
34439756	1	84	theme	good	224:227	arg1	sajor-caju					202:211	Lentinus (formerly Pleurotus) sajor-caju	172:211	Lentinus (formerly Pleurotus) sajor-caju (PSC)	172:217	The present study aimed to demonstrate Lentinus (formerly Pleurotus) sajor-caju (PSC) as a good source of pro-health substances.
34439756	1	84	theme	good	224:227	arg1	source					229:234	a good source	222:234	a good source of pro-health substances	222:259	The present study aimed to demonstrate Lentinus (formerly Pleurotus) sajor-caju (PSC) as a good source of pro-health substances.
33635456	0	0	theme	water	81:85	arg1	beads					123:127	modified water treatment residuals-sodium alginate beads	72:127	modified water treatment residuals-sodium alginate beads	72:127	Efficient removal of nitrogen and phosphorus in aqueous solutions using modified water treatment residuals-sodium alginate beads.
33635456	11	1	theme	domestic	1742:1749	arg1	wastewater					1766:1775	domestic and industrial wastewater	1742:1775	domestic and industrial wastewater	1742:1775	Based on these results, it can be concluded that the modified WTR-SA beads are a high efficiency adsorbent for removing nitrogen and phosphorus from domestic and industrial wastewater.
33635456	10	2	theme	WTR-SA	1435:1440	arg1	beads					1442:1446	modified WTR-SA beads	1426:1446	modified WTR-SA beads	1426:1446	While, the adsorption of modified WTR-SA beads on phosphorus is affected by three actions: ligand exchange, chemical precipitation, and ion exchange, which ligand exchange is the main effect.
33635456	1	3	theme	water	167:171	arg1	beads					209:213	modified water treatment residuals-sodium alginate beads	158:213	modified water treatment residuals-sodium alginate beads (WTR-SA beads)	158:228	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	1	3	theme	water	167:171	arg1	beads					223:227	WTR-SA beads	216:227	WTR-SA beads	216:227	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	1	3	theme	water	167:171	arg1	sorbent					149:155	A high-performance sorbent	130:155	A high-performance sorbent	130:155	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	6	4	theme	quasi-second-order	978:995	arg1	model					1005:1009	a quasi-second-order kinetic model	976:1009	a quasi-second-order kinetic model	976:1009	The adsorption behavior can be well described using a quasi-second-order kinetic model and Langmuir isotherm model.
33635456	0	5	theme	modified	72:79	arg1	beads					123:127	modified water treatment residuals-sodium alginate beads	72:127	modified water treatment residuals-sodium alginate beads	72:127	Efficient removal of nitrogen and phosphorus in aqueous solutions using modified water treatment residuals-sodium alginate beads.
33635456	0	6	from	removal	10:16	arg1	solutions					56:64	aqueous solutions	48:64	aqueous solutions using modified water treatment residuals-sodium alginate beads	48:127	Efficient removal of nitrogen and phosphorus in aqueous solutions using modified water treatment residuals-sodium alginate beads.
33635456	10	7	theme	modified	1426:1433	arg1	beads					1442:1446	modified WTR-SA beads	1426:1446	modified WTR-SA beads	1426:1446	While, the adsorption of modified WTR-SA beads on phosphorus is affected by three actions: ligand exchange, chemical precipitation, and ion exchange, which ligand exchange is the main effect.
33635456	1	8	theme	treatment	173:181	arg1	beads					209:213	modified water treatment residuals-sodium alginate beads	158:213	modified water treatment residuals-sodium alginate beads (WTR-SA beads)	158:228	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	1	8	theme	treatment	173:181	arg1	beads					223:227	WTR-SA beads	216:227	WTR-SA beads	216:227	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	1	8	theme	treatment	173:181	arg1	sorbent					149:155	A high-performance sorbent	130:155	A high-performance sorbent	130:155	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	0	9	theme	residuals-sodium	97:112	arg1	beads					123:127	modified water treatment residuals-sodium alginate beads	72:127	modified water treatment residuals-sodium alginate beads	72:127	Efficient removal of nitrogen and phosphorus in aqueous solutions using modified water treatment residuals-sodium alginate beads.
33635456	0	10	attach	removal	10:16	arg3	solutions					56:64	aqueous solutions	48:64	aqueous solutions using modified water treatment residuals-sodium alginate beads	48:127	Efficient removal of nitrogen and phosphorus in aqueous solutions using modified water treatment residuals-sodium alginate beads.
33635456	0	10	attach	removal	10:16	arg2	phosphorus					34:43	phosphorus	34:43	phosphorus	34:43	Efficient removal of nitrogen and phosphorus in aqueous solutions using modified water treatment residuals-sodium alginate beads.
33635456	0	10	attach	removal	10:16	arg2	nitrogen					21:28	nitrogen	21:28	nitrogen	21:28	Efficient removal of nitrogen and phosphorus in aqueous solutions using modified water treatment residuals-sodium alginate beads.
33635456	1	11	theme	salt	264:267	arg1	series					254:259	a series	252:259	a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate	252:374	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	0	12	theme	treatment	87:95	arg1	beads					123:127	modified water treatment residuals-sodium alginate beads	72:127	modified water treatment residuals-sodium alginate beads	72:127	Efficient removal of nitrogen and phosphorus in aqueous solutions using modified water treatment residuals-sodium alginate beads.
33635456	9	13	theme	hydroxyl	1335:1342	arg1	complexation					1344:1355	hydroxyl complexation	1335:1355	hydroxyl complexation	1335:1355	The adsorption of nitrogen by modified WTR-SA beads is mainly carried out through ion exchange and hydroxyl complexation, and ion exchange plays a major role in it.
33635456	11	14	theme	industrial	1755:1764	arg1	wastewater					1766:1775	domestic and industrial wastewater	1742:1775	domestic and industrial wastewater	1742:1775	Based on these results, it can be concluded that the modified WTR-SA beads are a high efficiency adsorbent for removing nitrogen and phosphorus from domestic and industrial wastewater.
33635456	3	15	theme	WTR-SA	510:515	arg1	beads					517:521	WTR-SA beads	510:521	WTR-SA beads	510:521	The adsorption performance of WTR-SA beads was investigated in removing nitrogen and phosphorus from wastewater.
33635456	8	16	theme	adsorption	1175:1184	arg1	process					1186:1192	the adsorption process	1171:1192	the adsorption process of phosphorus	1171:1206	On the contrary, the adsorption process of phosphorus is an endothermic reaction.
33635456	8	16	theme	adsorption	1175:1184	arg1	reaction					1226:1233	an endothermic reaction	1211:1233	an endothermic reaction	1211:1233	On the contrary, the adsorption process of phosphorus is an endothermic reaction.
33635456	8	17	from	reaction	1226:1233	arg1	contrary					1161:1168	the contrary	1157:1168	the contrary	1157:1168	On the contrary, the adsorption process of phosphorus is an endothermic reaction.
33635456	4	18	theme	modified	684:691	arg1	beads					700:704	the modified WTR-SA beads	680:704	the modified WTR-SA beads	680:704	Compared to the unmodified WTR, the removal efficiency of nitrogen and phosphorus onto the modified WTR-SA beads was increased from 22.34 and 77.13% to 95.14 and 98.31%, respectively.
33635456	1	19	theme	residuals-sodium	183:198	arg1	beads					209:213	modified water treatment residuals-sodium alginate beads	158:213	modified water treatment residuals-sodium alginate beads (WTR-SA beads)	158:228	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	1	19	theme	residuals-sodium	183:198	arg1	beads					223:227	WTR-SA beads	216:227	WTR-SA beads	216:227	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	1	19	theme	residuals-sodium	183:198	arg1	sorbent					149:155	A high-performance sorbent	130:155	A high-performance sorbent	130:155	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	0	20	theme	alginate	114:121	arg1	beads					123:127	modified water treatment residuals-sodium alginate beads	72:127	modified water treatment residuals-sodium alginate beads	72:127	Efficient removal of nitrogen and phosphorus in aqueous solutions using modified water treatment residuals-sodium alginate beads.
33635456	5	21	theme	mg/g	890:893	arg1	maximum					874:880	a maximum	872:880	a maximum of 2.52 mg/g and 6.45 mg/g	872:907	The adsorption capacities of nitrogen and phosphorus onto the modified WTR-SA beads were reach a maximum of 2.52 mg/g and 6.45 mg/g, respectively.
33635456	8	22	theme	endothermic	1214:1224	arg1	process					1186:1192	the adsorption process	1171:1192	the adsorption process of phosphorus	1171:1206	On the contrary, the adsorption process of phosphorus is an endothermic reaction.
33635456	8	22	theme	endothermic	1214:1224	arg1	reaction					1226:1233	an endothermic reaction	1211:1233	an endothermic reaction	1211:1233	On the contrary, the adsorption process of phosphorus is an endothermic reaction.
33635456	5	23	theme	nitrogen	806:813	arg1	capacities					792:801	The adsorption capacities	777:801	The adsorption capacities of nitrogen and phosphorus onto the modified WTR-SA beads	777:859	The adsorption capacities of nitrogen and phosphorus onto the modified WTR-SA beads were reach a maximum of 2.52 mg/g and 6.45 mg/g, respectively.
33635456	1	24	theme	alginate	200:207	arg1	beads					209:213	modified water treatment residuals-sodium alginate beads	158:213	modified water treatment residuals-sodium alginate beads (WTR-SA beads)	158:228	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	1	24	theme	alginate	200:207	arg1	beads					223:227	WTR-SA beads	216:227	WTR-SA beads	216:227	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	1	24	theme	alginate	200:207	arg1	sorbent					149:155	A high-performance sorbent	130:155	A high-performance sorbent	130:155	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	11	25	theme	WTR-SA	1655:1660	arg1	efficiency					1679:1688	a high efficiency	1672:1688	a high efficiency adsorbent for removing nitrogen and phosphorus from domestic and industrial wastewater	1672:1775	Based on these results, it can be concluded that the modified WTR-SA beads are a high efficiency adsorbent for removing nitrogen and phosphorus from domestic and industrial wastewater.
33635456	11	25	theme	WTR-SA	1655:1660	arg1	beads					1662:1666	the modified WTR-SA beads	1642:1666	the modified WTR-SA beads	1642:1666	Based on these results, it can be concluded that the modified WTR-SA beads are a high efficiency adsorbent for removing nitrogen and phosphorus from domestic and industrial wastewater.
33635456	10	26	theme	chemical	1509:1516	arg1	actions					1483:1489	three actions	1477:1489	three actions: ligand exchange, chemical precipitation, and ion exchange	1477:1548	While, the adsorption of modified WTR-SA beads on phosphorus is affected by three actions: ligand exchange, chemical precipitation, and ion exchange, which ligand exchange is the main effect.
33635456	10	26	theme	chemical	1509:1516	arg1	precipitation					1518:1530	chemical precipitation	1509:1530	chemical precipitation	1509:1530	While, the adsorption of modified WTR-SA beads on phosphorus is affected by three actions: ligand exchange, chemical precipitation, and ion exchange, which ligand exchange is the main effect.
33635456	7	27	theme	adsorption	1081:1090	arg1	properties					1058:1067	The thermodynamic properties	1040:1067	The thermodynamic properties of nitrogen adsorption	1040:1090	The thermodynamic properties of nitrogen adsorption indicated that the adsorption was spontaneous and exothermic.
33635456	4	28	theme	phosphorus	664:673	arg1	efficiency					637:646	the removal efficiency	625:646	the removal efficiency of nitrogen and phosphorus onto the modified WTR-SA beads	625:704	Compared to the unmodified WTR, the removal efficiency of nitrogen and phosphorus onto the modified WTR-SA beads was increased from 22.34 and 77.13% to 95.14 and 98.31%, respectively.
33635456	9	29	theme	ion	1318:1320	arg1	exchange					1322:1329	ion exchange	1318:1329	ion exchange	1318:1329	The adsorption of nitrogen by modified WTR-SA beads is mainly carried out through ion exchange and hydroxyl complexation, and ion exchange plays a major role in it.
33635456	2	30	theme	WTR-SA	404:409	arg1	composites					417:426	modified WTR-SA beads composites	395:426	modified WTR-SA beads composites	395:426	The properties of modified WTR-SA beads composites were characterized by SEM-EDS, FT-IR, XRD, and BET.
33635456	0	31	theme	Efficient	0:8	arg1	removal					10:16	Efficient removal	0:16	Efficient removal of nitrogen and phosphorus in aqueous solutions using modified water treatment residuals-sodium alginate beads.	0:128	Efficient removal of nitrogen and phosphorus in aqueous solutions using modified water treatment residuals-sodium alginate beads.
33635456	1	32	theme	combined	273:280	arg1	modification					309:320	combined thermal roasting composite modification	273:320	combined thermal roasting composite modification	273:320	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	10	33	theme	beads	1442:1446	arg1	adsorption					1412:1421	the adsorption	1408:1421	the adsorption of modified WTR-SA beads on phosphorus	1408:1460	While, the adsorption of modified WTR-SA beads on phosphorus is affected by three actions: ligand exchange, chemical precipitation, and ion exchange, which ligand exchange is the main effect.
33635456	2	34	theme	modified	395:402	arg1	composites					417:426	modified WTR-SA beads composites	395:426	modified WTR-SA beads composites	395:426	The properties of modified WTR-SA beads composites were characterized by SEM-EDS, FT-IR, XRD, and BET.
33635456	0	35	theme	nitrogen	21:28	arg1	removal					10:16	Efficient removal	0:16	Efficient removal of nitrogen and phosphorus in aqueous solutions using modified water treatment residuals-sodium alginate beads.	0:128	Efficient removal of nitrogen and phosphorus in aqueous solutions using modified water treatment residuals-sodium alginate beads.
33635456	1	36	theme	thermal	282:288	arg1	modification					309:320	combined thermal roasting composite modification	273:320	combined thermal roasting composite modification	273:320	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	5	37	theme	adsorption	781:790	arg1	capacities					792:801	The adsorption capacities	777:801	The adsorption capacities of nitrogen and phosphorus onto the modified WTR-SA beads	777:859	The adsorption capacities of nitrogen and phosphorus onto the modified WTR-SA beads were reach a maximum of 2.52 mg/g and 6.45 mg/g, respectively.
33635456	1	38	theme	WTR-SA	216:221	arg1	beads					209:213	modified water treatment residuals-sodium alginate beads	158:213	modified water treatment residuals-sodium alginate beads (WTR-SA beads)	158:228	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	1	38	theme	WTR-SA	216:221	arg1	beads					223:227	WTR-SA beads	216:227	WTR-SA beads	216:227	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	5	39	theme	phosphorus	819:828	arg1	capacities					792:801	The adsorption capacities	777:801	The adsorption capacities of nitrogen and phosphorus onto the modified WTR-SA beads	777:859	The adsorption capacities of nitrogen and phosphorus onto the modified WTR-SA beads were reach a maximum of 2.52 mg/g and 6.45 mg/g, respectively.
33635456	1	40	theme	roasting	290:297	arg1	modification					309:320	combined thermal roasting composite modification	273:320	combined thermal roasting composite modification	273:320	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	7	41	theme	nitrogen	1072:1079	arg1	adsorption					1081:1090	nitrogen adsorption	1072:1090	nitrogen adsorption	1072:1090	The thermodynamic properties of nitrogen adsorption indicated that the adsorption was spontaneous and exothermic.
33635456	11	42	theme	modified	1646:1653	arg1	efficiency					1679:1688	a high efficiency	1672:1688	a high efficiency adsorbent for removing nitrogen and phosphorus from domestic and industrial wastewater	1672:1775	Based on these results, it can be concluded that the modified WTR-SA beads are a high efficiency adsorbent for removing nitrogen and phosphorus from domestic and industrial wastewater.
33635456	11	42	theme	modified	1646:1653	arg1	beads					1662:1666	the modified WTR-SA beads	1642:1666	the modified WTR-SA beads	1642:1666	Based on these results, it can be concluded that the modified WTR-SA beads are a high efficiency adsorbent for removing nitrogen and phosphorus from domestic and industrial wastewater.
33635456	3	43	theme	beads	517:521	arg1	performance					495:505	The adsorption performance	480:505	The adsorption performance of WTR-SA beads	480:521	The adsorption performance of WTR-SA beads was investigated in removing nitrogen and phosphorus from wastewater.
33635456	0	44	theme	phosphorus	34:43	arg1	removal					10:16	Efficient removal	0:16	Efficient removal of nitrogen and phosphorus in aqueous solutions using modified water treatment residuals-sodium alginate beads.	0:128	Efficient removal of nitrogen and phosphorus in aqueous solutions using modified water treatment residuals-sodium alginate beads.
33635456	1	45	theme	composite	299:307	arg1	modification					309:320	combined thermal roasting composite modification	273:320	combined thermal roasting composite modification	273:320	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	7	46	theme	thermodynamic	1044:1056	arg1	properties					1058:1067	The thermodynamic properties	1040:1067	The thermodynamic properties of nitrogen adsorption	1040:1090	The thermodynamic properties of nitrogen adsorption indicated that the adsorption was spontaneous and exothermic.
33635456	10	47	theme	ligand	1557:1562	arg1	effect					1585:1590	the main effect	1576:1590	the main effect	1576:1590	While, the adsorption of modified WTR-SA beads on phosphorus is affected by three actions: ligand exchange, chemical precipitation, and ion exchange, which ligand exchange is the main effect.
33635456	10	47	theme	ligand	1557:1562	arg1	exchange					1564:1571	ligand exchange	1557:1571	ligand exchange	1557:1571	While, the adsorption of modified WTR-SA beads on phosphorus is affected by three actions: ligand exchange, chemical precipitation, and ion exchange, which ligand exchange is the main effect.
33635456	1	48	theme	sodium	360:365	arg1	alginate					367:374	sodium alginate	360:374	sodium alginate	360:374	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	5	49	theme	modified	839:846	arg1	beads					855:859	the modified WTR-SA beads	835:859	the modified WTR-SA beads	835:859	The adsorption capacities of nitrogen and phosphorus onto the modified WTR-SA beads were reach a maximum of 2.52 mg/g and 6.45 mg/g, respectively.
33635456	2	50	theme	composites	417:426	arg1	properties					381:390	The properties	377:390	The properties of modified WTR-SA beads composites	377:426	The properties of modified WTR-SA beads composites were characterized by SEM-EDS, FT-IR, XRD, and BET.
33635456	1	51	theme	modification	309:320	arg1	series					254:259	a series	252:259	a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate	252:374	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	4	52	theme	nitrogen	651:658	arg1	efficiency					637:646	the removal efficiency	625:646	the removal efficiency of nitrogen and phosphorus onto the modified WTR-SA beads	625:704	Compared to the unmodified WTR, the removal efficiency of nitrogen and phosphorus onto the modified WTR-SA beads was increased from 22.34 and 77.13% to 95.14 and 98.31%, respectively.
33635456	0	53	theme	aqueous	48:54	arg1	solutions					56:64	aqueous solutions	48:64	aqueous solutions using modified water treatment residuals-sodium alginate beads	48:127	Efficient removal of nitrogen and phosphorus in aqueous solutions using modified water treatment residuals-sodium alginate beads.
33635456	6	54	theme	adsorption	928:937	arg1	behavior					939:946	The adsorption behavior	924:946	The adsorption behavior	924:946	The adsorption behavior can be well described using a quasi-second-order kinetic model and Langmuir isotherm model.
33635456	6	55	theme	isotherm	1024:1031	arg1	model					1033:1037	Langmuir isotherm model	1015:1037	Langmuir isotherm model	1015:1037	The adsorption behavior can be well described using a quasi-second-order kinetic model and Langmuir isotherm model.
33635456	9	56	theme	nitrogen	1254:1261	arg1	adsorption					1240:1249	The adsorption	1236:1249	The adsorption of nitrogen by modified WTR-SA beads	1236:1286	The adsorption of nitrogen by modified WTR-SA beads is mainly carried out through ion exchange and hydroxyl complexation, and ion exchange plays a major role in it.
33635456	10	57	from	adsorption	1412:1421	arg1	phosphorus					1451:1460	phosphorus	1451:1460	phosphorus	1451:1460	While, the adsorption of modified WTR-SA beads on phosphorus is affected by three actions: ligand exchange, chemical precipitation, and ion exchange, which ligand exchange is the main effect.
33635456	10	58	dep	actions	1483:1489	arg1	exchange					1541:1548	ion exchange	1537:1548	ion exchange	1537:1548	While, the adsorption of modified WTR-SA beads on phosphorus is affected by three actions: ligand exchange, chemical precipitation, and ion exchange, which ligand exchange is the main effect.
33635456	10	58	dep	actions	1483:1489	arg1	actions					1483:1489	three actions	1477:1489	three actions: ligand exchange, chemical precipitation, and ion exchange	1477:1548	While, the adsorption of modified WTR-SA beads on phosphorus is affected by three actions: ligand exchange, chemical precipitation, and ion exchange, which ligand exchange is the main effect.
33635456	10	58	dep	actions	1483:1489	arg1	exchange					1499:1506	ligand exchange	1492:1506	ligand exchange	1492:1506	While, the adsorption of modified WTR-SA beads on phosphorus is affected by three actions: ligand exchange, chemical precipitation, and ion exchange, which ligand exchange is the main effect.
33635456	10	58	dep	actions	1483:1489	arg1	precipitation					1518:1530	chemical precipitation	1509:1530	chemical precipitation	1509:1530	While, the adsorption of modified WTR-SA beads on phosphorus is affected by three actions: ligand exchange, chemical precipitation, and ion exchange, which ligand exchange is the main effect.
33635456	10	59	theme	main	1580:1583	arg1	effect					1585:1590	the main effect	1576:1590	the main effect	1576:1590	While, the adsorption of modified WTR-SA beads on phosphorus is affected by three actions: ligand exchange, chemical precipitation, and ion exchange, which ligand exchange is the main effect.
33635456	10	59	theme	main	1580:1583	arg1	exchange					1564:1571	ligand exchange	1557:1571	ligand exchange	1557:1571	While, the adsorption of modified WTR-SA beads on phosphorus is affected by three actions: ligand exchange, chemical precipitation, and ion exchange, which ligand exchange is the main effect.
33635456	1	60	theme	water	330:334	arg1	residuals					346:354	water treatment residuals	330:354	water treatment residuals	330:354	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	11	61	theme	high	1674:1677	arg1	efficiency					1679:1688	a high efficiency	1672:1688	a high efficiency adsorbent for removing nitrogen and phosphorus from domestic and industrial wastewater	1672:1775	Based on these results, it can be concluded that the modified WTR-SA beads are a high efficiency adsorbent for removing nitrogen and phosphorus from domestic and industrial wastewater.
33635456	11	61	theme	high	1674:1677	arg1	beads					1662:1666	the modified WTR-SA beads	1642:1666	the modified WTR-SA beads	1642:1666	Based on these results, it can be concluded that the modified WTR-SA beads are a high efficiency adsorbent for removing nitrogen and phosphorus from domestic and industrial wastewater.
33635456	10	62	theme	ion	1537:1539	arg1	exchange					1541:1548	ion exchange	1537:1548	ion exchange	1537:1548	While, the adsorption of modified WTR-SA beads on phosphorus is affected by three actions: ligand exchange, chemical precipitation, and ion exchange, which ligand exchange is the main effect.
33635456	10	62	theme	ion	1537:1539	arg1	actions					1483:1489	three actions	1477:1489	three actions: ligand exchange, chemical precipitation, and ion exchange	1477:1548	While, the adsorption of modified WTR-SA beads on phosphorus is affected by three actions: ligand exchange, chemical precipitation, and ion exchange, which ligand exchange is the main effect.
33635456	1	63	theme	treatment	336:344	arg1	residuals					346:354	water treatment residuals	330:354	water treatment residuals	330:354	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	10	64	theme	ligand	1492:1497	arg1	actions					1483:1489	three actions	1477:1489	three actions: ligand exchange, chemical precipitation, and ion exchange	1477:1548	While, the adsorption of modified WTR-SA beads on phosphorus is affected by three actions: ligand exchange, chemical precipitation, and ion exchange, which ligand exchange is the main effect.
33635456	10	64	theme	ligand	1492:1497	arg1	exchange					1499:1506	ligand exchange	1492:1506	ligand exchange	1492:1506	While, the adsorption of modified WTR-SA beads on phosphorus is affected by three actions: ligand exchange, chemical precipitation, and ion exchange, which ligand exchange is the main effect.
33635456	9	65	theme	modified	1266:1273	arg1	beads					1282:1286	modified WTR-SA beads	1266:1286	modified WTR-SA beads	1266:1286	The adsorption of nitrogen by modified WTR-SA beads is mainly carried out through ion exchange and hydroxyl complexation, and ion exchange plays a major role in it.
33635456	4	66	theme	WTR-SA	693:698	arg1	beads					700:704	the modified WTR-SA beads	680:704	the modified WTR-SA beads	680:704	Compared to the unmodified WTR, the removal efficiency of nitrogen and phosphorus onto the modified WTR-SA beads was increased from 22.34 and 77.13% to 95.14 and 98.31%, respectively.
33635456	1	67	theme	high-performance	132:147	arg1	beads					209:213	modified water treatment residuals-sodium alginate beads	158:213	modified water treatment residuals-sodium alginate beads (WTR-SA beads)	158:228	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	1	67	theme	high-performance	132:147	arg1	sorbent					149:155	A high-performance sorbent	130:155	A high-performance sorbent	130:155	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	9	68	theme	major	1383:1387	arg1	role					1389:1392	a major role	1381:1392	a major role	1381:1392	The adsorption of nitrogen by modified WTR-SA beads is mainly carried out through ion exchange and hydroxyl complexation, and ion exchange plays a major role in it.
33635456	6	69	theme	Langmuir	1015:1022	arg1	model					1033:1037	Langmuir isotherm model	1015:1037	Langmuir isotherm model	1015:1037	The adsorption behavior can be well described using a quasi-second-order kinetic model and Langmuir isotherm model.
33635456	9	70	theme	WTR-SA	1275:1280	arg1	beads					1282:1286	modified WTR-SA beads	1266:1286	modified WTR-SA beads	1266:1286	The adsorption of nitrogen by modified WTR-SA beads is mainly carried out through ion exchange and hydroxyl complexation, and ion exchange plays a major role in it.
33635456	3	71	theme	adsorption	484:493	arg1	performance					495:505	The adsorption performance	480:505	The adsorption performance of WTR-SA beads	480:521	The adsorption performance of WTR-SA beads was investigated in removing nitrogen and phosphorus from wastewater.
33635456	9	72	theme	ion	1362:1364	arg1	exchange					1366:1373	ion exchange	1362:1373	ion exchange	1362:1373	The adsorption of nitrogen by modified WTR-SA beads is mainly carried out through ion exchange and hydroxyl complexation, and ion exchange plays a major role in it.
33635456	5	73	theme	mg/g	904:907	arg1	maximum					874:880	a maximum	872:880	a maximum of 2.52 mg/g and 6.45 mg/g	872:907	The adsorption capacities of nitrogen and phosphorus onto the modified WTR-SA beads were reach a maximum of 2.52 mg/g and 6.45 mg/g, respectively.
33635456	5	74	theme	WTR-SA	848:853	arg1	beads					855:859	the modified WTR-SA beads	835:859	the modified WTR-SA beads	835:859	The adsorption capacities of nitrogen and phosphorus onto the modified WTR-SA beads were reach a maximum of 2.52 mg/g and 6.45 mg/g, respectively.
33635456	11	75	theme	adsorbent	1690:1698	arg1	efficiency					1679:1688	a high efficiency	1672:1688	a high efficiency adsorbent for removing nitrogen and phosphorus from domestic and industrial wastewater	1672:1775	Based on these results, it can be concluded that the modified WTR-SA beads are a high efficiency adsorbent for removing nitrogen and phosphorus from domestic and industrial wastewater.
33635456	11	75	theme	adsorbent	1690:1698	arg1	beads					1662:1666	the modified WTR-SA beads	1642:1666	the modified WTR-SA beads	1642:1666	Based on these results, it can be concluded that the modified WTR-SA beads are a high efficiency adsorbent for removing nitrogen and phosphorus from domestic and industrial wastewater.
33635456	2	76	theme	beads	411:415	arg1	composites					417:426	modified WTR-SA beads composites	395:426	modified WTR-SA beads composites	395:426	The properties of modified WTR-SA beads composites were characterized by SEM-EDS, FT-IR, XRD, and BET.
33635456	4	77	theme	removal	629:635	arg1	efficiency					637:646	the removal efficiency	625:646	the removal efficiency of nitrogen and phosphorus onto the modified WTR-SA beads	625:704	Compared to the unmodified WTR, the removal efficiency of nitrogen and phosphorus onto the modified WTR-SA beads was increased from 22.34 and 77.13% to 95.14 and 98.31%, respectively.
33635456	4	78	theme	unmodified	609:618	arg1	WTR					620:622	the unmodified WTR	605:622	the unmodified WTR	605:622	Compared to the unmodified WTR, the removal efficiency of nitrogen and phosphorus onto the modified WTR-SA beads was increased from 22.34 and 77.13% to 95.14 and 98.31%, respectively.
33635456	8	79	theme	phosphorus	1197:1206	arg1	process					1186:1192	the adsorption process	1171:1192	the adsorption process of phosphorus	1171:1206	On the contrary, the adsorption process of phosphorus is an endothermic reaction.
33635456	8	79	theme	phosphorus	1197:1206	arg1	reaction					1226:1233	an endothermic reaction	1211:1233	an endothermic reaction	1211:1233	On the contrary, the adsorption process of phosphorus is an endothermic reaction.
33635456	1	80	theme	modified	158:165	arg1	beads					209:213	modified water treatment residuals-sodium alginate beads	158:213	modified water treatment residuals-sodium alginate beads (WTR-SA beads)	158:228	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	1	80	theme	modified	158:165	arg1	beads					223:227	WTR-SA beads	216:227	WTR-SA beads	216:227	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	1	80	theme	modified	158:165	arg1	sorbent					149:155	A high-performance sorbent	130:155	A high-performance sorbent	130:155	A high-performance sorbent, modified water treatment residuals-sodium alginate beads (WTR-SA beads), was prepared through a series of salt and combined thermal roasting composite modification between water treatment residuals and sodium alginate.
33635456	6	81	theme	kinetic	997:1003	arg1	model					1005:1009	a quasi-second-order kinetic model	976:1009	a quasi-second-order kinetic model	976:1009	The adsorption behavior can be well described using a quasi-second-order kinetic model and Langmuir isotherm model.
34294313	6	0	contain	contained	926:934	arg1	proliferation					860:872	the proliferation	856:872	the proliferation of NIH/3T3 cells significantly improved with 1% WGO	856:924	Moreover, the proliferation of NIH/3T3 cells significantly improved with 1% WGO contained hydrogels.
34294313	6	0	contain	contained	926:934	arg2	hydrogels					936:944	hydrogels	936:944	hydrogels	936:944	Moreover, the proliferation of NIH/3T3 cells significantly improved with 1% WGO contained hydrogels.
34294313	5	1	theme	no	664:665	arg1	WGO					667:669	no WGO	664:669	no WGO	664:669	Samples with 1% WGO and no WGO showed swelling of 5.9 and 10.5 g/g after 8 h. WGO inclusion resulted in reduced, but more stable fluid handling properties, with more uniform pore distribution (100-200 μm).
34294313	3	2	theme	fluid	475:479	arg1	properties					459:468	their physical properties	444:468	their physical properties	444:468	Following freeze-drying, textile-hydrogel composites have been examined according to their physical properties, pH, fluid handling capacity, water vapour permeability, morphology, chemical structure, and cytotoxicity.
34294313	3	2	theme	fluid	475:479	arg1	capacity					490:497	fluid handling capacity	475:497	fluid handling capacity	475:497	Following freeze-drying, textile-hydrogel composites have been examined according to their physical properties, pH, fluid handling capacity, water vapour permeability, morphology, chemical structure, and cytotoxicity.
34294313	5	3	dep	showed	671:676	arg1	resulted					732:739	resulted	732:739	resulted in reduced, but more stable fluid handling properties	732:793	Samples with 1% WGO and no WGO showed swelling of 5.9 and 10.5 g/g after 8 h. WGO inclusion resulted in reduced, but more stable fluid handling properties, with more uniform pore distribution (100-200 μm).
34294313	3	4	theme	handling	481:488	arg1	properties					459:468	their physical properties	444:468	their physical properties	444:468	Following freeze-drying, textile-hydrogel composites have been examined according to their physical properties, pH, fluid handling capacity, water vapour permeability, morphology, chemical structure, and cytotoxicity.
34294313	3	4	theme	handling	481:488	arg1	capacity					490:497	fluid handling capacity	475:497	fluid handling capacity	475:497	Following freeze-drying, textile-hydrogel composites have been examined according to their physical properties, pH, fluid handling capacity, water vapour permeability, morphology, chemical structure, and cytotoxicity.
34294313	5	5	theme	5.9	690:692	arg1	10.5 g/g					698:705	10.5 g/g	698:705	10.5 g/g after 8 h. WGO inclusion	698:730	Samples with 1% WGO and no WGO showed swelling of 5.9 and 10.5 g/g after 8 h. WGO inclusion resulted in reduced, but more stable fluid handling properties, with more uniform pore distribution (100-200 μm).
34294313	5	5	theme	5.9	690:692	arg1	swelling					678:685	swelling	678:685	swelling of 5.9	678:692	Samples with 1% WGO and no WGO showed swelling of 5.9 and 10.5 g/g after 8 h. WGO inclusion resulted in reduced, but more stable fluid handling properties, with more uniform pore distribution (100-200 μm).
34294313	2	6	theme	sodium	284:289	arg1	SA					301:302	SA	301:302	SA	301:302	Wheat germ oil (WGO) loaded hydrogels have successfully formed on textile nonwovens by cross-linking sodium alginate (SA) with poly(ethylene glycol) diglycidyl ether (PEGDGE).
34294313	2	6	theme	sodium	284:289	arg1	alginate					291:298	sodium alginate	284:298	sodium alginate (SA)	284:303	Wheat germ oil (WGO) loaded hydrogels have successfully formed on textile nonwovens by cross-linking sodium alginate (SA) with poly(ethylene glycol) diglycidyl ether (PEGDGE).
34294313	3	7	theme	chemical	539:546	arg1	properties					459:468	their physical properties	444:468	their physical properties	444:468	Following freeze-drying, textile-hydrogel composites have been examined according to their physical properties, pH, fluid handling capacity, water vapour permeability, morphology, chemical structure, and cytotoxicity.
34294313	3	7	theme	chemical	539:546	arg1	structure					548:556	chemical structure	539:556	chemical structure	539:556	Following freeze-drying, textile-hydrogel composites have been examined according to their physical properties, pH, fluid handling capacity, water vapour permeability, morphology, chemical structure, and cytotoxicity.
34294313	5	8	theme	1	653:653	arg1	%					654:654	%	654:654	%	654:654	Samples with 1% WGO and no WGO showed swelling of 5.9 and 10.5 g/g after 8 h. WGO inclusion resulted in reduced, but more stable fluid handling properties, with more uniform pore distribution (100-200 μm).
34294313	8	9	theme	water	1158:1162	arg1	permeability					1171:1182	water vapour permeability	1158:1182	water vapour permeability	1158:1182	The proposed product demonstrated 8.05 cm3/cm2/s and 541.37 g/m2/day air and water vapour permeability.
34294313	3	10	theme	water	500:504	arg1	permeability					513:524	water vapour permeability	500:524	water vapour permeability	500:524	Following freeze-drying, textile-hydrogel composites have been examined according to their physical properties, pH, fluid handling capacity, water vapour permeability, morphology, chemical structure, and cytotoxicity.
34294313	3	10	theme	water	500:504	arg1	properties					459:468	their physical properties	444:468	their physical properties	444:468	Following freeze-drying, textile-hydrogel composites have been examined according to their physical properties, pH, fluid handling capacity, water vapour permeability, morphology, chemical structure, and cytotoxicity.
34294313	5	11	theme	uniform	806:812	arg1	100-200 μm					833:842	100-200 μm	833:842	100-200 μm	833:842	Samples with 1% WGO and no WGO showed swelling of 5.9 and 10.5 g/g after 8 h. WGO inclusion resulted in reduced, but more stable fluid handling properties, with more uniform pore distribution (100-200 μm).
34294313	5	11	theme	uniform	806:812	arg1	distribution					819:830	more uniform pore distribution	801:830	more uniform pore distribution (100-200 μm)	801:843	Samples with 1% WGO and no WGO showed swelling of 5.9 and 10.5 g/g after 8 h. WGO inclusion resulted in reduced, but more stable fluid handling properties, with more uniform pore distribution (100-200 μm).
34294313	3	12	theme	freeze-drying	369:381	arg1	composites					401:410	Following freeze-drying, textile-hydrogel composites	359:410	Following freeze-drying, textile-hydrogel composites	359:410	Following freeze-drying, textile-hydrogel composites have been examined according to their physical properties, pH, fluid handling capacity, water vapour permeability, morphology, chemical structure, and cytotoxicity.
34294313	3	13	dep	freeze-drying	369:381	arg1	textile-hydrogel					384:399	textile-hydrogel	384:399	textile-hydrogel	384:399	Following freeze-drying, textile-hydrogel composites have been examined according to their physical properties, pH, fluid handling capacity, water vapour permeability, morphology, chemical structure, and cytotoxicity.
34294313	1	14	theme	composite	72:80	arg1	dressing					88:95	A composite wound dressing	70:95	A composite wound dressing	70:95	A composite wound dressing has been developed by combining different layers consisting of polymers and textiles.
34294313	5	15	theme	pore	814:817	arg1	100-200 μm					833:842	100-200 μm	833:842	100-200 μm	833:842	Samples with 1% WGO and no WGO showed swelling of 5.9 and 10.5 g/g after 8 h. WGO inclusion resulted in reduced, but more stable fluid handling properties, with more uniform pore distribution (100-200 μm).
34294313	5	15	theme	pore	814:817	arg1	distribution					819:830	more uniform pore distribution	801:830	more uniform pore distribution (100-200 μm)	801:843	Samples with 1% WGO and no WGO showed swelling of 5.9 and 10.5 g/g after 8 h. WGO inclusion resulted in reduced, but more stable fluid handling properties, with more uniform pore distribution (100-200 μm).
34294313	8	16	theme	vapour	1164:1169	arg1	permeability					1171:1182	water vapour permeability	1158:1182	water vapour permeability	1158:1182	The proposed product demonstrated 8.05 cm3/cm2/s and 541.37 g/m2/day air and water vapour permeability.
34294313	1	17	theme	wound	82:86	arg1	dressing					88:95	A composite wound dressing	70:95	A composite wound dressing	70:95	A composite wound dressing has been developed by combining different layers consisting of polymers and textiles.
34294313	5	18	theme	stable	762:767	arg1	properties					784:793	reduced, but more stable fluid handling properties	744:793	reduced, but more stable fluid handling properties	744:793	Samples with 1% WGO and no WGO showed swelling of 5.9 and 10.5 g/g after 8 h. WGO inclusion resulted in reduced, but more stable fluid handling properties, with more uniform pore distribution (100-200 μm).
34294313	0	19	theme	germ	20:23	arg1	oil					25:27	wheat germ oil	14:27	wheat germ oil	14:27	Production of wheat germ oil containing multilayer hydrogel dressing.
34294313	6	20	theme	%	920:920	arg1	WGO					922:924	1% WGO	919:924	1% WGO	919:924	Moreover, the proliferation of NIH/3T3 cells significantly improved with 1% WGO contained hydrogels.
34294313	7	21	theme	wound	1021:1025	arg1	area					1027:1030	the wound area	1017:1030	the wound area	1017:1030	Also, commercial self-adhesive dressings that secure the hydrogels to the wound area were investigated regarding transfer properties.
34294313	0	22	theme	wheat	14:18	arg1	oil					25:27	wheat germ oil	14:27	wheat germ oil	14:27	Production of wheat germ oil containing multilayer hydrogel dressing.
34294313	6	23	theme	1	919:919	arg1	%					920:920	%	920:920	%	920:920	Moreover, the proliferation of NIH/3T3 cells significantly improved with 1% WGO contained hydrogels.
34294313	5	24	with	Samples	640:646	arg1	WGO					656:658	1% WGO	653:658	1% WGO	653:658	Samples with 1% WGO and no WGO showed swelling of 5.9 and 10.5 g/g after 8 h. WGO inclusion resulted in reduced, but more stable fluid handling properties, with more uniform pore distribution (100-200 μm).
34294313	5	24	with	Samples	640:646	arg1	WGO					667:669	no WGO	664:669	no WGO	664:669	Samples with 1% WGO and no WGO showed swelling of 5.9 and 10.5 g/g after 8 h. WGO inclusion resulted in reduced, but more stable fluid handling properties, with more uniform pore distribution (100-200 μm).
34294313	2	25	theme	Wheat	183:187	arg1	oil					194:196	Wheat germ oil	183:196	Wheat germ oil (WGO) loaded hydrogels	183:219	Wheat germ oil (WGO) loaded hydrogels have successfully formed on textile nonwovens by cross-linking sodium alginate (SA) with poly(ethylene glycol) diglycidyl ether (PEGDGE).
34294313	2	25	theme	Wheat	183:187	arg1	WGO					199:201	WGO	199:201	WGO	199:201	Wheat germ oil (WGO) loaded hydrogels have successfully formed on textile nonwovens by cross-linking sodium alginate (SA) with poly(ethylene glycol) diglycidyl ether (PEGDGE).
34294313	2	26	theme	glycol	324:329	arg1	PEGDGE					350:355	PEGDGE	350:355	PEGDGE	350:355	Wheat germ oil (WGO) loaded hydrogels have successfully formed on textile nonwovens by cross-linking sodium alginate (SA) with poly(ethylene glycol) diglycidyl ether (PEGDGE).
34294313	2	26	theme	glycol	324:329	arg1	ether					343:347	poly(ethylene glycol) diglycidyl ether	310:347	poly(ethylene glycol) diglycidyl ether (PEGDGE)	310:356	Wheat germ oil (WGO) loaded hydrogels have successfully formed on textile nonwovens by cross-linking sodium alginate (SA) with poly(ethylene glycol) diglycidyl ether (PEGDGE).
34294313	3	27	theme	physical	450:457	arg1	cytotoxicity					563:574	cytotoxicity	563:574	cytotoxicity	563:574	Following freeze-drying, textile-hydrogel composites have been examined according to their physical properties, pH, fluid handling capacity, water vapour permeability, morphology, chemical structure, and cytotoxicity.
34294313	3	27	theme	physical	450:457	arg1	capacity					490:497	fluid handling capacity	475:497	fluid handling capacity	475:497	Following freeze-drying, textile-hydrogel composites have been examined according to their physical properties, pH, fluid handling capacity, water vapour permeability, morphology, chemical structure, and cytotoxicity.
34294313	3	27	theme	physical	450:457	arg1	morphology					527:536	morphology	527:536	morphology	527:536	Following freeze-drying, textile-hydrogel composites have been examined according to their physical properties, pH, fluid handling capacity, water vapour permeability, morphology, chemical structure, and cytotoxicity.
34294313	3	27	theme	physical	450:457	arg1	properties					459:468	their physical properties	444:468	their physical properties	444:468	Following freeze-drying, textile-hydrogel composites have been examined according to their physical properties, pH, fluid handling capacity, water vapour permeability, morphology, chemical structure, and cytotoxicity.
34294313	3	27	theme	physical	450:457	arg1	structure					548:556	chemical structure	539:556	chemical structure	539:556	Following freeze-drying, textile-hydrogel composites have been examined according to their physical properties, pH, fluid handling capacity, water vapour permeability, morphology, chemical structure, and cytotoxicity.
34294313	3	27	theme	physical	450:457	arg1	pH					471:472	pH	471:472	pH	471:472	Following freeze-drying, textile-hydrogel composites have been examined according to their physical properties, pH, fluid handling capacity, water vapour permeability, morphology, chemical structure, and cytotoxicity.
34294313	3	27	theme	physical	450:457	arg1	permeability					513:524	water vapour permeability	500:524	water vapour permeability	500:524	Following freeze-drying, textile-hydrogel composites have been examined according to their physical properties, pH, fluid handling capacity, water vapour permeability, morphology, chemical structure, and cytotoxicity.
34294313	0	28	theme	oil	25:27	arg1	Production					0:9	Production	0:9	Production of wheat germ oil	0:27	Production of wheat germ oil containing multilayer hydrogel dressing.
34294313	8	29	dep	8.05 cm3/cm2/s	1115:1128	arg1	air					1150:1152	air	1150:1152	air	1150:1152	The proposed product demonstrated 8.05 cm3/cm2/s and 541.37 g/m2/day air and water vapour permeability.
34294313	5	30	theme	reduced	744:750	arg1	properties					784:793	reduced, but more stable fluid handling properties	744:793	reduced, but more stable fluid handling properties	744:793	Samples with 1% WGO and no WGO showed swelling of 5.9 and 10.5 g/g after 8 h. WGO inclusion resulted in reduced, but more stable fluid handling properties, with more uniform pore distribution (100-200 μm).
34294313	5	31	theme	fluid	769:773	arg1	properties					784:793	reduced, but more stable fluid handling properties	744:793	reduced, but more stable fluid handling properties	744:793	Samples with 1% WGO and no WGO showed swelling of 5.9 and 10.5 g/g after 8 h. WGO inclusion resulted in reduced, but more stable fluid handling properties, with more uniform pore distribution (100-200 μm).
34294313	7	32	theme	self-adhesive	964:976	arg1	dressings					978:986	commercial self-adhesive dressings	953:986	commercial self-adhesive dressings that secure the hydrogels to the wound area	953:1030	Also, commercial self-adhesive dressings that secure the hydrogels to the wound area were investigated regarding transfer properties.
34294313	4	33	theme	pristine	620:627	arg1	hydrogels					629:637	pristine hydrogels	620:637	pristine hydrogels	620:637	Hydrogels containing WGO swelled less than pristine hydrogels.
34294313	0	34	theme	hydrogel	51:58	arg1	dressing					60:67	multilayer hydrogel dressing	40:67	multilayer hydrogel dressing	40:67	Production of wheat germ oil containing multilayer hydrogel dressing.
34294313	5	35	theme	%	654:654	arg1	WGO					656:658	1% WGO	653:658	1% WGO	653:658	Samples with 1% WGO and no WGO showed swelling of 5.9 and 10.5 g/g after 8 h. WGO inclusion resulted in reduced, but more stable fluid handling properties, with more uniform pore distribution (100-200 μm).
34294313	5	36	theme	WGO	718:720	arg1	inclusion					722:730	8 h. WGO inclusion	713:730	8 h. WGO inclusion	713:730	Samples with 1% WGO and no WGO showed swelling of 5.9 and 10.5 g/g after 8 h. WGO inclusion resulted in reduced, but more stable fluid handling properties, with more uniform pore distribution (100-200 μm).
34294313	2	37	theme	loaded	204:209	arg1	hydrogels					211:219	Wheat germ oil (WGO) loaded hydrogels	183:219	Wheat germ oil (WGO) loaded hydrogels	183:219	Wheat germ oil (WGO) loaded hydrogels have successfully formed on textile nonwovens by cross-linking sodium alginate (SA) with poly(ethylene glycol) diglycidyl ether (PEGDGE).
34294313	2	38	theme	ethylene	315:322	arg1	PEGDGE					350:355	PEGDGE	350:355	PEGDGE	350:355	Wheat germ oil (WGO) loaded hydrogels have successfully formed on textile nonwovens by cross-linking sodium alginate (SA) with poly(ethylene glycol) diglycidyl ether (PEGDGE).
34294313	2	38	theme	ethylene	315:322	arg1	ether					343:347	poly(ethylene glycol) diglycidyl ether	310:347	poly(ethylene glycol) diglycidyl ether (PEGDGE)	310:356	Wheat germ oil (WGO) loaded hydrogels have successfully formed on textile nonwovens by cross-linking sodium alginate (SA) with poly(ethylene glycol) diglycidyl ether (PEGDGE).
34294313	0	39	theme	multilayer	40:49	arg1	dressing					60:67	multilayer hydrogel dressing	40:67	multilayer hydrogel dressing	40:67	Production of wheat germ oil containing multilayer hydrogel dressing.
34294313	6	40	theme	cells	885:889	arg1	proliferation					860:872	the proliferation	856:872	the proliferation of NIH/3T3 cells significantly improved with 1% WGO	856:924	Moreover, the proliferation of NIH/3T3 cells significantly improved with 1% WGO contained hydrogels.
34294313	7	41	theme	transfer	1060:1067	arg1	properties					1069:1078	transfer properties	1060:1078	transfer properties	1060:1078	Also, commercial self-adhesive dressings that secure the hydrogels to the wound area were investigated regarding transfer properties.
34294313	6	42	theme	NIH/3T3	877:883	arg1	cells					885:889	NIH/3T3 cells	877:889	NIH/3T3 cells significantly improved with 1% WGO	877:924	Moreover, the proliferation of NIH/3T3 cells significantly improved with 1% WGO contained hydrogels.
34294313	8	43	theme	proposed	1085:1092	arg1	product					1094:1100	The proposed product	1081:1100	The proposed product	1081:1100	The proposed product demonstrated 8.05 cm3/cm2/s and 541.37 g/m2/day air and water vapour permeability.
34294313	2	44	theme	textile	249:255	arg1	nonwovens					257:265	textile nonwovens	249:265	textile nonwovens	249:265	Wheat germ oil (WGO) loaded hydrogels have successfully formed on textile nonwovens by cross-linking sodium alginate (SA) with poly(ethylene glycol) diglycidyl ether (PEGDGE).
34294313	7	45	theme	commercial	953:962	arg1	dressings					978:986	commercial self-adhesive dressings	953:986	commercial self-adhesive dressings that secure the hydrogels to the wound area	953:1030	Also, commercial self-adhesive dressings that secure the hydrogels to the wound area were investigated regarding transfer properties.
34294313	2	46	theme	poly	310:313	arg1	PEGDGE					350:355	PEGDGE	350:355	PEGDGE	350:355	Wheat germ oil (WGO) loaded hydrogels have successfully formed on textile nonwovens by cross-linking sodium alginate (SA) with poly(ethylene glycol) diglycidyl ether (PEGDGE).
34294313	2	46	theme	poly	310:313	arg1	ether					343:347	poly(ethylene glycol) diglycidyl ether	310:347	poly(ethylene glycol) diglycidyl ether (PEGDGE)	310:356	Wheat germ oil (WGO) loaded hydrogels have successfully formed on textile nonwovens by cross-linking sodium alginate (SA) with poly(ethylene glycol) diglycidyl ether (PEGDGE).
34294313	3	47	theme	vapour	506:511	arg1	permeability					513:524	water vapour permeability	500:524	water vapour permeability	500:524	Following freeze-drying, textile-hydrogel composites have been examined according to their physical properties, pH, fluid handling capacity, water vapour permeability, morphology, chemical structure, and cytotoxicity.
34294313	3	47	theme	vapour	506:511	arg1	properties					459:468	their physical properties	444:468	their physical properties	444:468	Following freeze-drying, textile-hydrogel composites have been examined according to their physical properties, pH, fluid handling capacity, water vapour permeability, morphology, chemical structure, and cytotoxicity.
34294313	2	48	theme	oil	194:196	arg1	hydrogels					211:219	Wheat germ oil (WGO) loaded hydrogels	183:219	Wheat germ oil (WGO) loaded hydrogels	183:219	Wheat germ oil (WGO) loaded hydrogels have successfully formed on textile nonwovens by cross-linking sodium alginate (SA) with poly(ethylene glycol) diglycidyl ether (PEGDGE).
34294313	5	49	theme	handling	775:782	arg1	properties					784:793	reduced, but more stable fluid handling properties	744:793	reduced, but more stable fluid handling properties	744:793	Samples with 1% WGO and no WGO showed swelling of 5.9 and 10.5 g/g after 8 h. WGO inclusion resulted in reduced, but more stable fluid handling properties, with more uniform pore distribution (100-200 μm).
34294313	1	50	theme	different	129:137	arg1	layers					139:144	different layers	129:144	different layers consisting of polymers and textiles	129:180	A composite wound dressing has been developed by combining different layers consisting of polymers and textiles.
34294313	5	51	theme	8 h.	713:716	arg1	inclusion					722:730	8 h. WGO inclusion	713:730	8 h. WGO inclusion	713:730	Samples with 1% WGO and no WGO showed swelling of 5.9 and 10.5 g/g after 8 h. WGO inclusion resulted in reduced, but more stable fluid handling properties, with more uniform pore distribution (100-200 μm).
34294313	2	52	theme	germ	189:192	arg1	oil					194:196	Wheat germ oil	183:196	Wheat germ oil (WGO) loaded hydrogels	183:219	Wheat germ oil (WGO) loaded hydrogels have successfully formed on textile nonwovens by cross-linking sodium alginate (SA) with poly(ethylene glycol) diglycidyl ether (PEGDGE).
34294313	2	52	theme	germ	189:192	arg1	WGO					199:201	WGO	199:201	WGO	199:201	Wheat germ oil (WGO) loaded hydrogels have successfully formed on textile nonwovens by cross-linking sodium alginate (SA) with poly(ethylene glycol) diglycidyl ether (PEGDGE).
34294313	3	53	theme	Following	359:367	arg1	composites					401:410	Following freeze-drying, textile-hydrogel composites	359:410	Following freeze-drying, textile-hydrogel composites	359:410	Following freeze-drying, textile-hydrogel composites have been examined according to their physical properties, pH, fluid handling capacity, water vapour permeability, morphology, chemical structure, and cytotoxicity.
34294313	4	54	contain	containing	587:596	arg2	WGO					598:600	WGO	598:600	WGO	598:600	Hydrogels containing WGO swelled less than pristine hydrogels.
34294313	4	54	contain	containing	587:596	arg1	Hydrogels					577:585	Hydrogels	577:585	Hydrogels containing WGO	577:600	Hydrogels containing WGO swelled less than pristine hydrogels.
34294313	2	55	theme	diglycidyl	332:341	arg1	PEGDGE					350:355	PEGDGE	350:355	PEGDGE	350:355	Wheat germ oil (WGO) loaded hydrogels have successfully formed on textile nonwovens by cross-linking sodium alginate (SA) with poly(ethylene glycol) diglycidyl ether (PEGDGE).
34294313	2	55	theme	diglycidyl	332:341	arg1	ether					343:347	poly(ethylene glycol) diglycidyl ether	310:347	poly(ethylene glycol) diglycidyl ether (PEGDGE)	310:356	Wheat germ oil (WGO) loaded hydrogels have successfully formed on textile nonwovens by cross-linking sodium alginate (SA) with poly(ethylene glycol) diglycidyl ether (PEGDGE).
32204107	10	0	dep	medium	1640:1645	arg1	OD590nm					1665:1671	OD590nm	1665:1671	OD590nm	1665:1671	The CA/CNT/Ag samples ensured effective bacterial growth inhibition on agar plates, in liquid medium (optical density, OD590nm) (for 48 h) and in bactericidal assay (relative cell viability, %).
32204107	10	0	dep	medium	1640:1645	arg1	density					1656:1662	optical density	1648:1662	optical density	1648:1662	The CA/CNT/Ag samples ensured effective bacterial growth inhibition on agar plates, in liquid medium (optical density, OD590nm) (for 48 h) and in bactericidal assay (relative cell viability, %).
32204107	10	1	theme	relative	1712:1719	arg1	%					1737:1737	%	1737:1737	%	1737:1737	The CA/CNT/Ag samples ensured effective bacterial growth inhibition on agar plates, in liquid medium (optical density, OD590nm) (for 48 h) and in bactericidal assay (relative cell viability, %).
32204107	10	1	theme	relative	1712:1719	arg1	viability					1726:1734	relative cell viability	1712:1734	relative cell viability	1712:1734	The CA/CNT/Ag samples ensured effective bacterial growth inhibition on agar plates, in liquid medium (optical density, OD590nm) (for 48 h) and in bactericidal assay (relative cell viability, %).
32204107	2	2	from	nanoparticles	367:379	arg1	CNTs					402:405	CNTs	402:405	CNTs	402:405	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	2	2	from	nanoparticles	367:379	arg1	nanotubes					391:399	carbon nanotubes	384:399	carbon nanotubes (CNTs)	384:406	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	6	3	theme	nanocomposite	1193:1205	arg1	Synthesis					1169:1177	Synthesis	1169:1177	Synthesis of the CNT/Ag nanocomposite	1169:1205	Synthesis of the CNT/Ag nanocomposite was confirmed with XPS, XRD, EDS and TEM analysis.
32204107	8	4	theme	nanoparticles	1374:1386	arg1	anchoring					1354:1362	anchoring	1354:1362	anchoring of silver nanoparticles	1354:1386	TEM images depicted anchoring of silver nanoparticles on CNTs and embedding of CNT/Ag in the CA nanofiber matrix.
32204107	3	5	theme	minimal/no	587:596	arg1	contact					605:611	minimal/no direct contact	587:611	minimal/no direct contact of silver nanoparticles with human cells	587:652	The carbon nanotubes/silver nanoparticles (CNT/Ag) nanocomposite localized inside the CA polymer matrix allow minimal/no direct contact of silver nanoparticles with human cells and are expected to show reduced silver leaching.
32204107	8	6	from	CNT/Ag	1413:1418	arg1	matrix					1440:1445	the CA nanofiber matrix	1423:1445	the CA nanofiber matrix	1423:1445	TEM images depicted anchoring of silver nanoparticles on CNTs and embedding of CNT/Ag in the CA nanofiber matrix.
32204107	2	7	theme	harmful	283:289	arg1	effects					291:297	harmful effects	283:297	harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix	283:474	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	10	8	theme	growth	1596:1601	arg1	inhibition					1603:1612	effective bacterial growth inhibition	1576:1612	effective bacterial growth inhibition	1576:1612	The CA/CNT/Ag samples ensured effective bacterial growth inhibition on agar plates, in liquid medium (optical density, OD590nm) (for 48 h) and in bactericidal assay (relative cell viability, %).
32204107	3	9	with	contact	605:611	arg1	cells					648:652	human cells	642:652	human cells	642:652	The carbon nanotubes/silver nanoparticles (CNT/Ag) nanocomposite localized inside the CA polymer matrix allow minimal/no direct contact of silver nanoparticles with human cells and are expected to show reduced silver leaching.
32204107	8	10	from	embedding	1400:1408	arg1	matrix					1440:1445	the CA nanofiber matrix	1423:1445	the CA nanofiber matrix	1423:1445	TEM images depicted anchoring of silver nanoparticles on CNTs and embedding of CNT/Ag in the CA nanofiber matrix.
32204107	4	11	theme	acetate	718:724	arg1	nanofibers					731:740	The cellulose acetate (CA) nanofibers	704:740	The cellulose acetate (CA) nanofibers loaded with silver nanoparticles anchored multiwall carbon nanotubes (CNT/Ag)	704:818	The cellulose acetate (CA) nanofibers loaded with silver nanoparticles anchored multiwall carbon nanotubes (CNT/Ag) were fabricated by electrospinning.
32204107	11	12	theme	potential	1797:1805	arg1	candidate					1807:1815	potential candidate	1797:1815	potential candidate for safer antibacterial applications	1797:1852	Our results suggested CA/CNT/Ag composite nanofibers as potential candidate for safer antibacterial applications.
32204107	3	13	theme	silver	616:621	arg1	nanoparticles					623:635	silver nanoparticles	616:635	silver nanoparticles	616:635	The carbon nanotubes/silver nanoparticles (CNT/Ag) nanocomposite localized inside the CA polymer matrix allow minimal/no direct contact of silver nanoparticles with human cells and are expected to show reduced silver leaching.
32204107	9	14	theme	antibacterial	1452:1464	arg1	results					1471:1477	The antibacterial test results	1448:1477	The antibacterial test results	1448:1477	The antibacterial test results demonstrated excellent antibacterial performance of the CA/CNT/Ag.
32204107	8	15	theme	silver	1367:1372	arg1	nanoparticles					1374:1386	silver nanoparticles	1367:1386	silver nanoparticles	1367:1386	TEM images depicted anchoring of silver nanoparticles on CNTs and embedding of CNT/Ag in the CA nanofiber matrix.
32204107	5	16	theme	scanning	886:893	arg1	microscopy					904:913	scanning electron microscopy	886:913	scanning electron microscopy (SEM)	886:919	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	16	theme	scanning	886:893	arg1	diffraction					928:938	X-ray diffraction	922:938	X-ray diffraction (XRD)	922:944	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	16	theme	scanning	886:893	arg1	spectroscopy					967:978	X-ray photoelectron spectroscopy	947:978	X-ray photoelectron spectroscopy (XPS)	947:984	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	16	theme	scanning	886:893	arg1	microscopy					1053:1062	transmission electron microscopy	1031:1062	transmission electron microscopy (TEM)	1031:1068	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	16	theme	scanning	886:893	arg1	SEM					916:918	SEM	916:918	SEM	916:918	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	16	theme	scanning	886:893	arg1	spectroscopy					1011:1022	energy dispersive X-ray spectroscopy	987:1022	energy dispersive X-ray spectroscopy (EDS)	987:1028	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	10	17	theme	optical	1648:1654	arg1	OD590nm					1665:1671	OD590nm	1665:1671	OD590nm	1665:1671	The CA/CNT/Ag samples ensured effective bacterial growth inhibition on agar plates, in liquid medium (optical density, OD590nm) (for 48 h) and in bactericidal assay (relative cell viability, %).
32204107	10	17	theme	optical	1648:1654	arg1	density					1656:1662	optical density	1648:1662	optical density	1648:1662	The CA/CNT/Ag samples ensured effective bacterial growth inhibition on agar plates, in liquid medium (optical density, OD590nm) (for 48 h) and in bactericidal assay (relative cell viability, %).
32204107	11	18	theme	antibacterial	1827:1839	arg1	applications					1841:1852	safer antibacterial applications	1821:1852	safer antibacterial applications	1821:1852	Our results suggested CA/CNT/Ag composite nanofibers as potential candidate for safer antibacterial applications.
32204107	5	19	theme	infra-red	1089:1097	arg1	FTIR					1113:1116	FTIR	1113:1116	FTIR	1113:1116	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	19	theme	infra-red	1089:1097	arg1	tests					1137:1141	tensile strength tests	1120:1141	tensile strength tests	1120:1141	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	19	theme	infra-red	1089:1097	arg1	assays					1161:1166	antibacterial assays	1147:1166	antibacterial assays	1147:1166	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	19	theme	infra-red	1089:1097	arg1	spectroscopy					1099:1110	infra-red spectroscopy	1089:1110	infra-red spectroscopy (FTIR)	1089:1117	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	9	20	theme	excellent	1492:1500	arg1	performance					1516:1526	excellent antibacterial performance	1492:1526	excellent antibacterial performance of the CA/CNT/Ag	1492:1543	The antibacterial test results demonstrated excellent antibacterial performance of the CA/CNT/Ag.
32204107	3	21	dep	inside	552:557	arg1	allow					581:585	allow	581:585	allow minimal/no direct contact of silver nanoparticles with human cells	581:652	The carbon nanotubes/silver nanoparticles (CNT/Ag) nanocomposite localized inside the CA polymer matrix allow minimal/no direct contact of silver nanoparticles with human cells and are expected to show reduced silver leaching.
32204107	3	21	dep	inside	552:557	arg1	expected					662:669	expected	662:669	are expected to show reduced silver leaching	658:701	The carbon nanotubes/silver nanoparticles (CNT/Ag) nanocomposite localized inside the CA polymer matrix allow minimal/no direct contact of silver nanoparticles with human cells and are expected to show reduced silver leaching.
32204107	2	22	theme	i.e.	310:313	arg1	argyria					315:321	i.e. argyria	310:321	i.e. argyria	310:321	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	5	23	theme	photoelectron	953:965	arg1	XPS					981:983	XPS	981:983	XPS	981:983	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	23	theme	photoelectron	953:965	arg1	spectroscopy					967:978	X-ray photoelectron spectroscopy	947:978	X-ray photoelectron spectroscopy (XPS)	947:984	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	23	theme	photoelectron	953:965	arg1	microscopy					904:913	scanning electron microscopy	886:913	scanning electron microscopy (SEM)	886:919	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	24	theme	tensile	1120:1126	arg1	tests					1137:1141	tensile strength tests	1120:1141	tensile strength tests	1120:1141	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	24	theme	tensile	1120:1126	arg1	spectroscopy					1099:1110	infra-red spectroscopy	1089:1110	infra-red spectroscopy (FTIR)	1089:1117	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	2	25	theme	silver	302:307	arg1	effects					291:297	harmful effects	283:297	harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix	283:474	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	1	26	theme	composite	195:203	arg1	CA/CNT/Ag					174:182	CA/CNT/Ag	174:182	CA/CNT/Ag	174:182	Herein we propose cellulose acetate/carbon nanotube/silver nanoparticles (CA/CNT/Ag) nanofiber composite for antibacterial applications.
32204107	1	26	theme	composite	195:203	arg1	nanoparticles					159:171	cellulose acetate/carbon nanotube/silver nanoparticles	118:171	cellulose acetate/carbon nanotube/silver nanoparticles (CA/CNT/Ag) nanofiber composite for antibacterial applications	118:234	Herein we propose cellulose acetate/carbon nanotube/silver nanoparticles (CA/CNT/Ag) nanofiber composite for antibacterial applications.
32204107	2	27	from	anchoring	347:355	arg1	CNTs					402:405	CNTs	402:405	CNTs	402:405	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	2	27	from	anchoring	347:355	arg1	nanotubes					391:399	carbon nanotubes	384:399	carbon nanotubes (CNTs)	384:406	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	8	28	theme	TEM	1334:1336	arg1	images					1338:1343	TEM images	1334:1343	TEM images	1334:1343	TEM images depicted anchoring of silver nanoparticles on CNTs and embedding of CNT/Ag in the CA nanofiber matrix.
32204107	11	29	theme	CA/CNT/Ag	1763:1771	arg1	nanofibers					1783:1792	CA/CNT/Ag composite nanofibers	1763:1792	CA/CNT/Ag composite nanofibers	1763:1792	Our results suggested CA/CNT/Ag composite nanofibers as potential candidate for safer antibacterial applications.
32204107	8	30	from	matrix	1440:1445	arg1	embedding					1400:1408	embedding	1400:1408	embedding of CNT/Ag in the CA nanofiber matrix	1400:1445	TEM images depicted anchoring of silver nanoparticles on CNTs and embedding of CNT/Ag in the CA nanofiber matrix.
32204107	8	30	from	matrix	1440:1445	arg1	CNTs					1391:1394	CNTs	1391:1394	CNTs	1391:1394	TEM images depicted anchoring of silver nanoparticles on CNTs and embedding of CNT/Ag in the CA nanofiber matrix.
32204107	1	31	theme	antibacterial	209:221	arg1	applications					223:234	antibacterial applications	209:234	antibacterial applications	209:234	Herein we propose cellulose acetate/carbon nanotube/silver nanoparticles (CA/CNT/Ag) nanofiber composite for antibacterial applications.
32204107	10	32	dep	assay	1705:1709	arg1	%					1737:1737	%	1737:1737	%	1737:1737	The CA/CNT/Ag samples ensured effective bacterial growth inhibition on agar plates, in liquid medium (optical density, OD590nm) (for 48 h) and in bactericidal assay (relative cell viability, %).
32204107	10	32	dep	assay	1705:1709	arg1	viability					1726:1734	relative cell viability	1712:1734	relative cell viability	1712:1734	The CA/CNT/Ag samples ensured effective bacterial growth inhibition on agar plates, in liquid medium (optical density, OD590nm) (for 48 h) and in bactericidal assay (relative cell viability, %).
32204107	5	33	theme	energy	987:992	arg1	EDS					1025:1027	EDS	1025:1027	EDS	1025:1027	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	33	theme	energy	987:992	arg1	spectroscopy					1011:1022	energy dispersive X-ray spectroscopy	987:1022	energy dispersive X-ray spectroscopy (EDS)	987:1028	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	33	theme	energy	987:992	arg1	microscopy					904:913	scanning electron microscopy	886:913	scanning electron microscopy (SEM)	886:919	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	2	34	theme	composite	429:437	arg1	matrix					469:474	the composite inside cellulose acetate (CA) matrix	425:474	the composite inside cellulose acetate (CA) matrix	425:474	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	5	35	theme	X-ray	1005:1009	arg1	EDS					1025:1027	EDS	1025:1027	EDS	1025:1027	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	35	theme	X-ray	1005:1009	arg1	spectroscopy					1011:1022	energy dispersive X-ray spectroscopy	987:1022	energy dispersive X-ray spectroscopy (EDS)	987:1028	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	35	theme	X-ray	1005:1009	arg1	microscopy					904:913	scanning electron microscopy	886:913	scanning electron microscopy (SEM)	886:919	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	4	36	theme	multiwall	784:792	arg1	CNT/Ag					812:817	CNT/Ag	812:817	CNT/Ag	812:817	The cellulose acetate (CA) nanofibers loaded with silver nanoparticles anchored multiwall carbon nanotubes (CNT/Ag) were fabricated by electrospinning.
32204107	4	36	theme	multiwall	784:792	arg1	nanotubes					801:809	anchored multiwall carbon nanotubes	775:809	silver nanoparticles anchored multiwall carbon nanotubes (CNT/Ag)	754:818	The cellulose acetate (CA) nanofibers loaded with silver nanoparticles anchored multiwall carbon nanotubes (CNT/Ag) were fabricated by electrospinning.
32204107	0	37	theme	Cellulose	0:8	arg1	nanotube/Ag					36:46	Cellulose acetate/multi-wall carbon nanotube/Ag	0:46	Cellulose acetate/multi-wall carbon nanotube/Ag	0:46	Cellulose acetate/multi-wall carbon nanotube/Ag nanofiber composite for antibacterial applications.
32204107	1	38	theme	cellulose	118:126	arg1	CA/CNT/Ag					174:182	CA/CNT/Ag	174:182	CA/CNT/Ag	174:182	Herein we propose cellulose acetate/carbon nanotube/silver nanoparticles (CA/CNT/Ag) nanofiber composite for antibacterial applications.
32204107	1	38	theme	cellulose	118:126	arg1	nanoparticles					159:171	cellulose acetate/carbon nanotube/silver nanoparticles	118:171	cellulose acetate/carbon nanotube/silver nanoparticles (CA/CNT/Ag) nanofiber composite for antibacterial applications	118:234	Herein we propose cellulose acetate/carbon nanotube/silver nanoparticles (CA/CNT/Ag) nanofiber composite for antibacterial applications.
32204107	7	39	theme	composites	1322:1331	arg1	morphology					1284:1293	regular morphology	1276:1293	regular morphology of the CA/CNT/Ag nanofiber composites	1276:1331	SEM images showed regular morphology of the CA/CNT/Ag nanofiber composites.
32204107	3	40	theme	nanotubes/silver	488:503	arg1	CNT/Ag					520:525	CNT/Ag	520:525	CNT/Ag	520:525	The carbon nanotubes/silver nanoparticles (CNT/Ag) nanocomposite localized inside the CA polymer matrix allow minimal/no direct contact of silver nanoparticles with human cells and are expected to show reduced silver leaching.
32204107	3	40	theme	nanotubes/silver	488:503	arg1	nanoparticles					505:517	The carbon nanotubes/silver nanoparticles	477:517	The carbon nanotubes/silver nanoparticles (CNT/Ag)	477:526	The carbon nanotubes/silver nanoparticles (CNT/Ag) nanocomposite localized inside the CA polymer matrix allow minimal/no direct contact of silver nanoparticles with human cells and are expected to show reduced silver leaching.
32204107	0	41	theme	carbon	29:34	arg1	nanotube/Ag					36:46	Cellulose acetate/multi-wall carbon nanotube/Ag	0:46	Cellulose acetate/multi-wall carbon nanotube/Ag	0:46	Cellulose acetate/multi-wall carbon nanotube/Ag nanofiber composite for antibacterial applications.
32204107	1	42	theme	nanotube/silver	143:157	arg1	CA/CNT/Ag					174:182	CA/CNT/Ag	174:182	CA/CNT/Ag	174:182	Herein we propose cellulose acetate/carbon nanotube/silver nanoparticles (CA/CNT/Ag) nanofiber composite for antibacterial applications.
32204107	1	42	theme	nanotube/silver	143:157	arg1	nanoparticles					159:171	cellulose acetate/carbon nanotube/silver nanoparticles	118:171	cellulose acetate/carbon nanotube/silver nanoparticles (CA/CNT/Ag) nanofiber composite for antibacterial applications	118:234	Herein we propose cellulose acetate/carbon nanotube/silver nanoparticles (CA/CNT/Ag) nanofiber composite for antibacterial applications.
32204107	7	43	theme	CA/CNT/Ag	1302:1310	arg1	composites					1322:1331	the CA/CNT/Ag nanofiber composites	1298:1331	the CA/CNT/Ag nanofiber composites	1298:1331	SEM images showed regular morphology of the CA/CNT/Ag nanofiber composites.
32204107	2	44	theme	cellulose	446:454	arg1	matrix					469:474	the composite inside cellulose acetate (CA) matrix	425:474	the composite inside cellulose acetate (CA) matrix	425:474	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	5	45	theme	antibacterial	1147:1159	arg1	assays					1161:1166	antibacterial assays	1147:1166	antibacterial assays	1147:1166	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	45	theme	antibacterial	1147:1159	arg1	spectroscopy					1099:1110	infra-red spectroscopy	1089:1110	infra-red spectroscopy (FTIR)	1089:1117	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	46	theme	transmission	1031:1042	arg1	microscopy					904:913	scanning electron microscopy	886:913	scanning electron microscopy (SEM)	886:919	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	46	theme	transmission	1031:1042	arg1	TEM					1065:1067	TEM	1065:1067	TEM	1065:1067	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	46	theme	transmission	1031:1042	arg1	microscopy					1053:1062	transmission electron microscopy	1031:1062	transmission electron microscopy (TEM)	1031:1068	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	47	theme	X-ray	922:926	arg1	microscopy					904:913	scanning electron microscopy	886:913	scanning electron microscopy (SEM)	886:919	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	47	theme	X-ray	922:926	arg1	XRD					941:943	XRD	941:943	XRD	941:943	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	47	theme	X-ray	922:926	arg1	diffraction					928:938	X-ray diffraction	922:938	X-ray diffraction (XRD)	922:944	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	10	48	theme	CA/CNT/Ag	1550:1558	arg1	samples					1560:1566	The CA/CNT/Ag samples	1546:1566	The CA/CNT/Ag samples	1546:1566	The CA/CNT/Ag samples ensured effective bacterial growth inhibition on agar plates, in liquid medium (optical density, OD590nm) (for 48 h) and in bactericidal assay (relative cell viability, %).
32204107	3	49	theme	reduced	679:685	arg1	leaching					694:701	reduced silver leaching	679:701	reduced silver leaching	679:701	The carbon nanotubes/silver nanoparticles (CNT/Ag) nanocomposite localized inside the CA polymer matrix allow minimal/no direct contact of silver nanoparticles with human cells and are expected to show reduced silver leaching.
32204107	8	50	theme	CNT/Ag	1413:1418	arg1	embedding					1400:1408	embedding	1400:1408	embedding of CNT/Ag in the CA nanofiber matrix	1400:1445	TEM images depicted anchoring of silver nanoparticles on CNTs and embedding of CNT/Ag in the CA nanofiber matrix.
32204107	8	50	theme	CNT/Ag	1413:1418	arg1	CNTs					1391:1394	CNTs	1391:1394	CNTs	1391:1394	TEM images depicted anchoring of silver nanoparticles on CNTs and embedding of CNT/Ag in the CA nanofiber matrix.
32204107	7	51	theme	regular	1276:1282	arg1	morphology					1284:1293	regular morphology	1276:1293	regular morphology of the CA/CNT/Ag nanofiber composites	1276:1331	SEM images showed regular morphology of the CA/CNT/Ag nanofiber composites.
32204107	10	52	theme	bactericidal	1692:1703	arg1	assay					1705:1709	bactericidal assay	1692:1709	bactericidal assay (relative cell viability, %)	1692:1738	The CA/CNT/Ag samples ensured effective bacterial growth inhibition on agar plates, in liquid medium (optical density, OD590nm) (for 48 h) and in bactericidal assay (relative cell viability, %).
32204107	10	53	theme	bacterial	1586:1594	arg1	inhibition					1603:1612	effective bacterial growth inhibition	1576:1612	effective bacterial growth inhibition	1576:1612	The CA/CNT/Ag samples ensured effective bacterial growth inhibition on agar plates, in liquid medium (optical density, OD590nm) (for 48 h) and in bactericidal assay (relative cell viability, %).
32204107	3	54	theme	carbon	481:486	arg1	CNT/Ag					520:525	CNT/Ag	520:525	CNT/Ag	520:525	The carbon nanotubes/silver nanoparticles (CNT/Ag) nanocomposite localized inside the CA polymer matrix allow minimal/no direct contact of silver nanoparticles with human cells and are expected to show reduced silver leaching.
32204107	3	54	theme	carbon	481:486	arg1	nanoparticles					505:517	The carbon nanotubes/silver nanoparticles	477:517	The carbon nanotubes/silver nanoparticles (CNT/Ag)	477:526	The carbon nanotubes/silver nanoparticles (CNT/Ag) nanocomposite localized inside the CA polymer matrix allow minimal/no direct contact of silver nanoparticles with human cells and are expected to show reduced silver leaching.
32204107	8	55	theme	nanofiber	1430:1438	arg1	matrix					1440:1445	the CA nanofiber matrix	1423:1445	the CA nanofiber matrix	1423:1445	TEM images depicted anchoring of silver nanoparticles on CNTs and embedding of CNT/Ag in the CA nanofiber matrix.
32204107	10	56	theme	cell	1721:1724	arg1	%					1737:1737	%	1737:1737	%	1737:1737	The CA/CNT/Ag samples ensured effective bacterial growth inhibition on agar plates, in liquid medium (optical density, OD590nm) (for 48 h) and in bactericidal assay (relative cell viability, %).
32204107	10	56	theme	cell	1721:1724	arg1	viability					1726:1734	relative cell viability	1712:1734	relative cell viability	1712:1734	The CA/CNT/Ag samples ensured effective bacterial growth inhibition on agar plates, in liquid medium (optical density, OD590nm) (for 48 h) and in bactericidal assay (relative cell viability, %).
32204107	10	57	theme	agar	1617:1620	arg1	plates					1622:1627	agar plates	1617:1627	agar plates	1617:1627	The CA/CNT/Ag samples ensured effective bacterial growth inhibition on agar plates, in liquid medium (optical density, OD590nm) (for 48 h) and in bactericidal assay (relative cell viability, %).
32204107	2	58	from	nanotubes	391:399	arg1	anchoring					347:355	anchoring	347:355	anchoring of silver nanoparticles on carbon nanotubes (CNTs)	347:406	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	6	59	theme	CNT/Ag	1186:1191	arg1	nanocomposite					1193:1205	the CNT/Ag nanocomposite	1182:1205	the CNT/Ag nanocomposite	1182:1205	Synthesis of the CNT/Ag nanocomposite was confirmed with XPS, XRD, EDS and TEM analysis.
32204107	3	60	theme	polymer	566:572	arg1	matrix					574:579	the CA polymer matrix	559:579	the CA polymer matrix	559:579	The carbon nanotubes/silver nanoparticles (CNT/Ag) nanocomposite localized inside the CA polymer matrix allow minimal/no direct contact of silver nanoparticles with human cells and are expected to show reduced silver leaching.
32204107	2	61	dep	silver	302:307	arg1	argyrosis					327:335	argyrosis	327:335	argyrosis	327:335	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	2	61	dep	silver	302:307	arg1	argyria					315:321	i.e. argyria	310:321	i.e. argyria	310:321	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	10	62	theme	liquid	1633:1638	arg1	medium					1640:1645	liquid medium	1633:1645	liquid medium (optical density, OD590nm) (for 48 h)	1633:1683	The CA/CNT/Ag samples ensured effective bacterial growth inhibition on agar plates, in liquid medium (optical density, OD590nm) (for 48 h) and in bactericidal assay (relative cell viability, %).
32204107	6	63	theme	TEM	1244:1246	arg1	analysis					1248:1255	TEM analysis	1244:1255	TEM analysis	1244:1255	Synthesis of the CNT/Ag nanocomposite was confirmed with XPS, XRD, EDS and TEM analysis.
32204107	2	64	theme	silver	360:365	arg1	nanoparticles					367:379	silver nanoparticles	360:379	silver nanoparticles on carbon nanotubes (CNTs)	360:406	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	4	65	theme	cellulose	708:716	arg1	CA					727:728	CA	727:728	CA	727:728	The cellulose acetate (CA) nanofibers loaded with silver nanoparticles anchored multiwall carbon nanotubes (CNT/Ag) were fabricated by electrospinning.
32204107	4	65	theme	cellulose	708:716	arg1	acetate					718:724	cellulose acetate	708:724	The cellulose acetate (CA) nanofibers loaded with silver nanoparticles anchored multiwall carbon nanotubes (CNT/Ag)	704:818	The cellulose acetate (CA) nanofibers loaded with silver nanoparticles anchored multiwall carbon nanotubes (CNT/Ag) were fabricated by electrospinning.
32204107	3	66	theme	direct	598:603	arg1	contact					605:611	minimal/no direct contact	587:611	minimal/no direct contact of silver nanoparticles with human cells	587:652	The carbon nanotubes/silver nanoparticles (CNT/Ag) nanocomposite localized inside the CA polymer matrix allow minimal/no direct contact of silver nanoparticles with human cells and are expected to show reduced silver leaching.
32204107	9	67	theme	test	1466:1469	arg1	results					1471:1477	The antibacterial test results	1448:1477	The antibacterial test results	1448:1477	The antibacterial test results demonstrated excellent antibacterial performance of the CA/CNT/Ag.
32204107	3	68	theme	human	642:646	arg1	cells					648:652	human cells	642:652	human cells	642:652	The carbon nanotubes/silver nanoparticles (CNT/Ag) nanocomposite localized inside the CA polymer matrix allow minimal/no direct contact of silver nanoparticles with human cells and are expected to show reduced silver leaching.
32204107	5	69	theme	electron	895:902	arg1	microscopy					904:913	scanning electron microscopy	886:913	scanning electron microscopy (SEM)	886:919	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	69	theme	electron	895:902	arg1	diffraction					928:938	X-ray diffraction	922:938	X-ray diffraction (XRD)	922:944	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	69	theme	electron	895:902	arg1	spectroscopy					967:978	X-ray photoelectron spectroscopy	947:978	X-ray photoelectron spectroscopy (XPS)	947:984	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	69	theme	electron	895:902	arg1	microscopy					1053:1062	transmission electron microscopy	1031:1062	transmission electron microscopy (TEM)	1031:1068	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	69	theme	electron	895:902	arg1	SEM					916:918	SEM	916:918	SEM	916:918	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	69	theme	electron	895:902	arg1	spectroscopy					1011:1022	energy dispersive X-ray spectroscopy	987:1022	energy dispersive X-ray spectroscopy (EDS)	987:1028	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	3	70	theme	nanoparticles	623:635	arg1	contact					605:611	minimal/no direct contact	587:611	minimal/no direct contact of silver nanoparticles with human cells	587:652	The carbon nanotubes/silver nanoparticles (CNT/Ag) nanocomposite localized inside the CA polymer matrix allow minimal/no direct contact of silver nanoparticles with human cells and are expected to show reduced silver leaching.
32204107	9	71	theme	antibacterial	1502:1514	arg1	performance					1516:1526	excellent antibacterial performance	1492:1526	excellent antibacterial performance of the CA/CNT/Ag	1492:1543	The antibacterial test results demonstrated excellent antibacterial performance of the CA/CNT/Ag.
32204107	11	72	theme	safer	1821:1825	arg1	applications					1841:1852	safer antibacterial applications	1821:1852	safer antibacterial applications	1821:1852	Our results suggested CA/CNT/Ag composite nanofibers as potential candidate for safer antibacterial applications.
32204107	5	73	theme	X-ray	947:951	arg1	XPS					981:983	XPS	981:983	XPS	981:983	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	73	theme	X-ray	947:951	arg1	spectroscopy					967:978	X-ray photoelectron spectroscopy	947:978	X-ray photoelectron spectroscopy (XPS)	947:984	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	73	theme	X-ray	947:951	arg1	microscopy					904:913	scanning electron microscopy	886:913	scanning electron microscopy (SEM)	886:919	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	4	74	theme	silver	754:759	arg1	nanoparticles					761:773	silver nanoparticles anchored multiwall carbon nanotubes (CNT/Ag)	754:818	silver nanoparticles anchored multiwall carbon nanotubes (CNT/Ag)	754:818	The cellulose acetate (CA) nanofibers loaded with silver nanoparticles anchored multiwall carbon nanotubes (CNT/Ag) were fabricated by electrospinning.
32204107	9	75	theme	CA/CNT/Ag	1535:1543	arg1	performance					1516:1526	excellent antibacterial performance	1492:1526	excellent antibacterial performance of the CA/CNT/Ag	1492:1543	The antibacterial test results demonstrated excellent antibacterial performance of the CA/CNT/Ag.
32204107	5	76	theme	strength	1128:1135	arg1	tests					1137:1141	tensile strength tests	1120:1141	tensile strength tests	1120:1141	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	76	theme	strength	1128:1135	arg1	spectroscopy					1099:1110	infra-red spectroscopy	1089:1110	infra-red spectroscopy (FTIR)	1089:1117	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	11	77	theme	composite	1773:1781	arg1	nanofibers					1783:1792	CA/CNT/Ag composite nanofibers	1763:1792	CA/CNT/Ag composite nanofibers	1763:1792	Our results suggested CA/CNT/Ag composite nanofibers as potential candidate for safer antibacterial applications.
32204107	5	78	theme	dispersive	994:1003	arg1	EDS					1025:1027	EDS	1025:1027	EDS	1025:1027	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	78	theme	dispersive	994:1003	arg1	spectroscopy					1011:1022	energy dispersive X-ray spectroscopy	987:1022	energy dispersive X-ray spectroscopy (EDS)	987:1028	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	78	theme	dispersive	994:1003	arg1	microscopy					904:913	scanning electron microscopy	886:913	scanning electron microscopy (SEM)	886:919	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	0	79	theme	acetate/multi-wall	10:27	arg1	nanotube/Ag					36:46	Cellulose acetate/multi-wall carbon nanotube/Ag	0:46	Cellulose acetate/multi-wall carbon nanotube/Ag	0:46	Cellulose acetate/multi-wall carbon nanotube/Ag nanofiber composite for antibacterial applications.
32204107	3	80	dep	localized	542:550	arg1	inside					552:557	inside	552:557	inside the CA polymer matrix allow minimal/no direct contact of silver nanoparticles with human cells and are expected to show reduced silver leaching	552:701	The carbon nanotubes/silver nanoparticles (CNT/Ag) nanocomposite localized inside the CA polymer matrix allow minimal/no direct contact of silver nanoparticles with human cells and are expected to show reduced silver leaching.
32204107	7	81	theme	nanofiber	1312:1320	arg1	composites					1322:1331	the CA/CNT/Ag nanofiber composites	1298:1331	the CA/CNT/Ag nanofiber composites	1298:1331	SEM images showed regular morphology of the CA/CNT/Ag nanofiber composites.
32204107	2	82	theme	matrix	469:474	arg1	effects					291:297	harmful effects	283:297	harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix	283:474	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	4	83	theme	anchored	775:782	arg1	CNT/Ag					812:817	CNT/Ag	812:817	CNT/Ag	812:817	The cellulose acetate (CA) nanofibers loaded with silver nanoparticles anchored multiwall carbon nanotubes (CNT/Ag) were fabricated by electrospinning.
32204107	4	83	theme	anchored	775:782	arg1	nanotubes					801:809	anchored multiwall carbon nanotubes	775:809	silver nanoparticles anchored multiwall carbon nanotubes (CNT/Ag)	754:818	The cellulose acetate (CA) nanofibers loaded with silver nanoparticles anchored multiwall carbon nanotubes (CNT/Ag) were fabricated by electrospinning.
32204107	2	84	theme	embedding	412:420	arg1	effects					291:297	harmful effects	283:297	harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix	283:474	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	1	85	theme	acetate/carbon	128:141	arg1	CA/CNT/Ag					174:182	CA/CNT/Ag	174:182	CA/CNT/Ag	174:182	Herein we propose cellulose acetate/carbon nanotube/silver nanoparticles (CA/CNT/Ag) nanofiber composite for antibacterial applications.
32204107	1	85	theme	acetate/carbon	128:141	arg1	nanoparticles					159:171	cellulose acetate/carbon nanotube/silver nanoparticles	118:171	cellulose acetate/carbon nanotube/silver nanoparticles (CA/CNT/Ag) nanofiber composite for antibacterial applications	118:234	Herein we propose cellulose acetate/carbon nanotube/silver nanoparticles (CA/CNT/Ag) nanofiber composite for antibacterial applications.
32204107	2	86	theme	CA	465:466	arg1	matrix					469:474	the composite inside cellulose acetate (CA) matrix	425:474	the composite inside cellulose acetate (CA) matrix	425:474	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	3	87	theme	silver	687:692	arg1	leaching					694:701	reduced silver leaching	679:701	reduced silver leaching	679:701	The carbon nanotubes/silver nanoparticles (CNT/Ag) nanocomposite localized inside the CA polymer matrix allow minimal/no direct contact of silver nanoparticles with human cells and are expected to show reduced silver leaching.
32204107	4	88	theme	carbon	794:799	arg1	CNT/Ag					812:817	CNT/Ag	812:817	CNT/Ag	812:817	The cellulose acetate (CA) nanofibers loaded with silver nanoparticles anchored multiwall carbon nanotubes (CNT/Ag) were fabricated by electrospinning.
32204107	4	88	theme	carbon	794:799	arg1	nanotubes					801:809	anchored multiwall carbon nanotubes	775:809	silver nanoparticles anchored multiwall carbon nanotubes (CNT/Ag)	754:818	The cellulose acetate (CA) nanofibers loaded with silver nanoparticles anchored multiwall carbon nanotubes (CNT/Ag) were fabricated by electrospinning.
32204107	2	89	theme	acetate	456:462	arg1	matrix					469:474	the composite inside cellulose acetate (CA) matrix	425:474	the composite inside cellulose acetate (CA) matrix	425:474	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	2	90	theme	inside	439:444	arg1	matrix					469:474	the composite inside cellulose acetate (CA) matrix	425:474	the composite inside cellulose acetate (CA) matrix	425:474	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	0	91	theme	antibacterial	72:84	arg1	applications					86:97	antibacterial applications	72:97	antibacterial applications	72:97	Cellulose acetate/multi-wall carbon nanotube/Ag nanofiber composite for antibacterial applications.
32204107	5	92	theme	electron	1044:1051	arg1	microscopy					904:913	scanning electron microscopy	886:913	scanning electron microscopy (SEM)	886:919	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	92	theme	electron	1044:1051	arg1	TEM					1065:1067	TEM	1065:1067	TEM	1065:1067	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	5	92	theme	electron	1044:1051	arg1	microscopy					1053:1062	transmission electron microscopy	1031:1062	transmission electron microscopy (TEM)	1031:1068	The samples were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR), tensile strength tests and antibacterial assays.
32204107	2	93	theme	carbon	384:389	arg1	CNTs					402:405	CNTs	402:405	CNTs	402:405	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	2	93	theme	carbon	384:389	arg1	nanotubes					391:399	carbon nanotubes	384:399	carbon nanotubes (CNTs)	384:406	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	4	94	dep	nanoparticles	761:773	arg1	CNT/Ag					812:817	CNT/Ag	812:817	CNT/Ag	812:817	The cellulose acetate (CA) nanofibers loaded with silver nanoparticles anchored multiwall carbon nanotubes (CNT/Ag) were fabricated by electrospinning.
32204107	4	94	dep	nanoparticles	761:773	arg1	nanotubes					801:809	anchored multiwall carbon nanotubes	775:809	silver nanoparticles anchored multiwall carbon nanotubes (CNT/Ag)	754:818	The cellulose acetate (CA) nanofibers loaded with silver nanoparticles anchored multiwall carbon nanotubes (CNT/Ag) were fabricated by electrospinning.
32204107	7	95	theme	SEM	1258:1260	arg1	images					1262:1267	SEM images	1258:1267	SEM images	1258:1267	SEM images showed regular morphology of the CA/CNT/Ag nanofiber composites.
32204107	2	96	theme	nanoparticles	367:379	arg1	anchoring					347:355	anchoring	347:355	anchoring of silver nanoparticles on carbon nanotubes (CNTs)	347:406	The nanofiber composite are expected to avoid harmful effects of silver (i.e. argyria and argyrosis) owing to anchoring of silver nanoparticles on carbon nanotubes (CNTs) and embedding of the composite inside cellulose acetate (CA) matrix.
32204107	10	97	theme	effective	1576:1584	arg1	inhibition					1603:1612	effective bacterial growth inhibition	1576:1612	effective bacterial growth inhibition	1576:1612	The CA/CNT/Ag samples ensured effective bacterial growth inhibition on agar plates, in liquid medium (optical density, OD590nm) (for 48 h) and in bactericidal assay (relative cell viability, %).
32204107	8	98	theme	CA	1427:1428	arg1	matrix					1440:1445	the CA nanofiber matrix	1423:1445	the CA nanofiber matrix	1423:1445	TEM images depicted anchoring of silver nanoparticles on CNTs and embedding of CNT/Ag in the CA nanofiber matrix.
32204107	3	99	theme	CA	563:564	arg1	matrix					574:579	the CA polymer matrix	559:579	the CA polymer matrix	559:579	The carbon nanotubes/silver nanoparticles (CNT/Ag) nanocomposite localized inside the CA polymer matrix allow minimal/no direct contact of silver nanoparticles with human cells and are expected to show reduced silver leaching.
32204107	8	100	from	CNTs	1391:1394	arg1	matrix					1440:1445	the CA nanofiber matrix	1423:1445	the CA nanofiber matrix	1423:1445	TEM images depicted anchoring of silver nanoparticles on CNTs and embedding of CNT/Ag in the CA nanofiber matrix.
34297578	0	0	theme	Nanocrystals	101:112	arg1	Postsynthesis					0:12	Postsynthesis Self- And Coassembly	0:33	Postsynthesis Self- And Coassembly of Enzymatically Produced Fluorinated Cellodextrins and Cellulose Nanocrystals.	0:113	Postsynthesis Self- And Coassembly of Enzymatically Produced Fluorinated Cellodextrins and Cellulose Nanocrystals.
34297578	0	0	theme	Nanocrystals	101:112	arg1	Coassembly					24:33	Coassembly	24:33	Coassembly	24:33	Postsynthesis Self- And Coassembly of Enzymatically Produced Fluorinated Cellodextrins and Cellulose Nanocrystals.
34297578	0	0	theme	Nanocrystals	101:112	arg1	Self-					14:18	Self-	14:18	Self-	14:18	Postsynthesis Self- And Coassembly of Enzymatically Produced Fluorinated Cellodextrins and Cellulose Nanocrystals.
34297578	4	1	theme	postsynthesis	547:559	arg1	assembly					561:568	postsynthesis assembly	547:568	postsynthesis assembly of the cellodextrins	547:589	Highly ordered 2D structures of large lateral dimensions, unattainable in the polymerization process, can be formed because of postsynthesis assembly of the cellodextrins.
34297578	0	2	theme	Cellulose	91:99	arg1	Nanocrystals					101:112	Cellulose Nanocrystals	91:112	Cellulose Nanocrystals	91:112	Postsynthesis Self- And Coassembly of Enzymatically Produced Fluorinated Cellodextrins and Cellulose Nanocrystals.
34297578	4	3	theme	large	452:456	arg1	dimensions					466:475	large lateral dimensions	452:475	large lateral dimensions	452:475	Highly ordered 2D structures of large lateral dimensions, unattainable in the polymerization process, can be formed because of postsynthesis assembly of the cellodextrins.
34297578	3	4	theme	cellodextrins	405:417	arg1	assembly					375:382	postsynthesis assembly	361:382	postsynthesis assembly of novel fluorinated cellodextrins	361:417	Here we demonstrate the feasibility of postsynthesis assembly of novel fluorinated cellodextrins.
34297578	4	5	theme	dimensions	466:475	arg1	structures					438:447	Highly ordered 2D structures	420:447	Highly ordered 2D structures	420:447	Highly ordered 2D structures of large lateral dimensions, unattainable in the polymerization process, can be formed because of postsynthesis assembly of the cellodextrins.
34297578	3	6	theme	novel	387:391	arg1	cellodextrins					405:417	novel fluorinated cellodextrins	387:417	novel fluorinated cellodextrins	387:417	Here we demonstrate the feasibility of postsynthesis assembly of novel fluorinated cellodextrins.
34297578	4	7	theme	lateral	458:464	arg1	dimensions					466:475	large lateral dimensions	452:475	large lateral dimensions	452:475	Highly ordered 2D structures of large lateral dimensions, unattainable in the polymerization process, can be formed because of postsynthesis assembly of the cellodextrins.
34297578	2	8	theme	polymers	312:319	arg1	synthesis					284:292	the enzymatic synthesis	270:292	the enzymatic synthesis of cellulose-like polymers	270:319	Formation of 2D platelets is known in the enzymatic synthesis of cellulose-like polymers.
34297578	6	9	theme	atoms	775:779	arg1	content					755:761	the content	751:761	the content of fluorine atoms in the fluorinated cellodextrins	751:812	The hybrid architectures obtained depend on the content of fluorine atoms in the fluorinated cellodextrins.
34297578	3	10	theme	fluorinated	393:403	arg1	cellodextrins					405:417	novel fluorinated cellodextrins	387:417	novel fluorinated cellodextrins	387:417	Here we demonstrate the feasibility of postsynthesis assembly of novel fluorinated cellodextrins.
34297578	5	11	theme	hybrid	691:696	arg1	systems					698:704	hybrid systems	691:704	hybrid systems	691:704	These cellodextrins were also involved in coassembly with cellulose nanocrystals (CNCs) leading to hybrid systems.
34297578	4	12	theme	ordered	427:433	arg1	structures					438:447	Highly ordered 2D structures	420:447	Highly ordered 2D structures	420:447	Highly ordered 2D structures of large lateral dimensions, unattainable in the polymerization process, can be formed because of postsynthesis assembly of the cellodextrins.
34297578	2	13	theme	cellulose-like	297:310	arg1	polymers					312:319	cellulose-like polymers	297:319	cellulose-like polymers	297:319	Formation of 2D platelets is known in the enzymatic synthesis of cellulose-like polymers.
34297578	6	14	theme	fluorine	766:773	arg1	atoms					775:779	fluorine atoms	766:779	fluorine atoms	766:779	The hybrid architectures obtained depend on the content of fluorine atoms in the fluorinated cellodextrins.
34297578	3	15	theme	postsynthesis	361:373	arg1	assembly					375:382	postsynthesis assembly	361:382	postsynthesis assembly of novel fluorinated cellodextrins	361:417	Here we demonstrate the feasibility of postsynthesis assembly of novel fluorinated cellodextrins.
34297578	1	16	theme	hierarchical	207:218	arg1	structures					220:229	hierarchical structures	207:229	hierarchical structures	207:229	The design of new functional materials and devices substantially relies on self-assembly of hierarchical structures.
34297578	7	17	theme	multifluorinated	888:903	arg1	cellodextrins					905:917	multifluorinated cellodextrins	888:917	multifluorinated cellodextrins	888:917	Monofluorinated cellodextrins coassemble with CNCs into a nanoweb, while multifluorinated cellodextrins assemble around the CNCs.
34297578	5	18	theme	cellulose	650:658	arg1	CNCs					674:677	CNCs	674:677	CNCs	674:677	These cellodextrins were also involved in coassembly with cellulose nanocrystals (CNCs) leading to hybrid systems.
34297578	5	18	theme	cellulose	650:658	arg1	nanocrystals					660:671	cellulose nanocrystals	650:671	cellulose nanocrystals (CNCs) leading to hybrid systems	650:704	These cellodextrins were also involved in coassembly with cellulose nanocrystals (CNCs) leading to hybrid systems.
34297578	4	19	theme	unattainable	478:489	arg1	structures					438:447	Highly ordered 2D structures	420:447	Highly ordered 2D structures	420:447	Highly ordered 2D structures of large lateral dimensions, unattainable in the polymerization process, can be formed because of postsynthesis assembly of the cellodextrins.
34297578	1	20	theme	structures	220:229	arg1	self-assembly					190:202	self-assembly	190:202	self-assembly of hierarchical structures	190:229	The design of new functional materials and devices substantially relies on self-assembly of hierarchical structures.
34297578	4	21	theme	2D	435:436	arg1	structures					438:447	Highly ordered 2D structures	420:447	Highly ordered 2D structures	420:447	Highly ordered 2D structures of large lateral dimensions, unattainable in the polymerization process, can be formed because of postsynthesis assembly of the cellodextrins.
34297578	6	22	from	content	755:761	arg1	cellodextrins					800:812	the fluorinated cellodextrins	784:812	the fluorinated cellodextrins	784:812	The hybrid architectures obtained depend on the content of fluorine atoms in the fluorinated cellodextrins.
34297578	5	23	with	coassembly	634:643	arg1	CNCs					674:677	CNCs	674:677	CNCs	674:677	These cellodextrins were also involved in coassembly with cellulose nanocrystals (CNCs) leading to hybrid systems.
34297578	5	23	with	coassembly	634:643	arg1	nanocrystals					660:671	cellulose nanocrystals	650:671	cellulose nanocrystals (CNCs) leading to hybrid systems	650:704	These cellodextrins were also involved in coassembly with cellulose nanocrystals (CNCs) leading to hybrid systems.
34297578	4	24	from	process	513:519	arg1	unattainable					478:489	unattainable	478:489	unattainable	478:489	Highly ordered 2D structures of large lateral dimensions, unattainable in the polymerization process, can be formed because of postsynthesis assembly of the cellodextrins.
34297578	4	25	theme	polymerization	498:511	arg1	process					513:519	the polymerization process	494:519	the polymerization process	494:519	Highly ordered 2D structures of large lateral dimensions, unattainable in the polymerization process, can be formed because of postsynthesis assembly of the cellodextrins.
34297578	0	26	theme	Fluorinated	61:71	arg1	Cellodextrins					73:85	Enzymatically Produced Fluorinated Cellodextrins	38:85	Enzymatically Produced Fluorinated Cellodextrins	38:85	Postsynthesis Self- And Coassembly of Enzymatically Produced Fluorinated Cellodextrins and Cellulose Nanocrystals.
34297578	4	27	theme	cellodextrins	577:589	arg1	assembly					561:568	postsynthesis assembly	547:568	postsynthesis assembly of the cellodextrins	547:589	Highly ordered 2D structures of large lateral dimensions, unattainable in the polymerization process, can be formed because of postsynthesis assembly of the cellodextrins.
34297578	1	28	theme	new	129:131	arg1	materials					144:152	new functional materials	129:152	new functional materials	129:152	The design of new functional materials and devices substantially relies on self-assembly of hierarchical structures.
34297578	0	29	theme	Produced	52:59	arg1	Cellodextrins					73:85	Enzymatically Produced Fluorinated Cellodextrins	38:85	Enzymatically Produced Fluorinated Cellodextrins	38:85	Postsynthesis Self- And Coassembly of Enzymatically Produced Fluorinated Cellodextrins and Cellulose Nanocrystals.
34297578	2	30	theme	2D	245:246	arg1	platelets					248:256	2D platelets	245:256	2D platelets	245:256	Formation of 2D platelets is known in the enzymatic synthesis of cellulose-like polymers.
34297578	2	31	theme	platelets	248:256	arg1	Formation					232:240	Formation	232:240	Formation of 2D platelets	232:256	Formation of 2D platelets is known in the enzymatic synthesis of cellulose-like polymers.
34297578	1	32	theme	functional	133:142	arg1	materials					144:152	new functional materials	129:152	new functional materials	129:152	The design of new functional materials and devices substantially relies on self-assembly of hierarchical structures.
34297578	4	33	from	unattainable	478:489	arg1	process					513:519	the polymerization process	494:519	the polymerization process	494:519	Highly ordered 2D structures of large lateral dimensions, unattainable in the polymerization process, can be formed because of postsynthesis assembly of the cellodextrins.
34297578	1	34	theme	materials	144:152	arg1	design					119:124	The design	115:124	The design of new functional materials and devices	115:164	The design of new functional materials and devices substantially relies on self-assembly of hierarchical structures.
34297578	6	35	theme	hybrid	711:716	arg1	architectures					718:730	The hybrid architectures	707:730	The hybrid architectures obtained	707:739	The hybrid architectures obtained depend on the content of fluorine atoms in the fluorinated cellodextrins.
34297578	3	36	theme	assembly	375:382	arg1	feasibility					346:356	the feasibility	342:356	the feasibility of postsynthesis assembly of novel fluorinated cellodextrins	342:417	Here we demonstrate the feasibility of postsynthesis assembly of novel fluorinated cellodextrins.
34297578	2	37	theme	enzymatic	274:282	arg1	synthesis					284:292	the enzymatic synthesis	270:292	the enzymatic synthesis of cellulose-like polymers	270:319	Formation of 2D platelets is known in the enzymatic synthesis of cellulose-like polymers.
34297578	1	38	theme	devices	158:164	arg1	design					119:124	The design	115:124	The design of new functional materials and devices	115:164	The design of new functional materials and devices substantially relies on self-assembly of hierarchical structures.
34297578	7	39	theme	Monofluorinated	815:829	arg1	cellodextrins					831:843	Monofluorinated cellodextrins	815:843	Monofluorinated cellodextrins	815:843	Monofluorinated cellodextrins coassemble with CNCs into a nanoweb, while multifluorinated cellodextrins assemble around the CNCs.
34297578	0	40	theme	Cellodextrins	73:85	arg1	Postsynthesis					0:12	Postsynthesis Self- And Coassembly	0:33	Postsynthesis Self- And Coassembly of Enzymatically Produced Fluorinated Cellodextrins and Cellulose Nanocrystals.	0:113	Postsynthesis Self- And Coassembly of Enzymatically Produced Fluorinated Cellodextrins and Cellulose Nanocrystals.
34297578	0	40	theme	Cellodextrins	73:85	arg1	Coassembly					24:33	Coassembly	24:33	Coassembly	24:33	Postsynthesis Self- And Coassembly of Enzymatically Produced Fluorinated Cellodextrins and Cellulose Nanocrystals.
34297578	0	40	theme	Cellodextrins	73:85	arg1	Self-					14:18	Self-	14:18	Self-	14:18	Postsynthesis Self- And Coassembly of Enzymatically Produced Fluorinated Cellodextrins and Cellulose Nanocrystals.
34297578	0	41	dep	Postsynthesis	0:12	arg1	Postsynthesis					0:12	Postsynthesis Self- And Coassembly	0:33	Postsynthesis Self- And Coassembly of Enzymatically Produced Fluorinated Cellodextrins and Cellulose Nanocrystals.	0:113	Postsynthesis Self- And Coassembly of Enzymatically Produced Fluorinated Cellodextrins and Cellulose Nanocrystals.
34297578	0	41	dep	Postsynthesis	0:12	arg1	Coassembly					24:33	Coassembly	24:33	Coassembly	24:33	Postsynthesis Self- And Coassembly of Enzymatically Produced Fluorinated Cellodextrins and Cellulose Nanocrystals.
34297578	0	41	dep	Postsynthesis	0:12	arg1	Self-					14:18	Self-	14:18	Self-	14:18	Postsynthesis Self- And Coassembly of Enzymatically Produced Fluorinated Cellodextrins and Cellulose Nanocrystals.
34297578	6	42	theme	fluorinated	788:798	arg1	cellodextrins					800:812	the fluorinated cellodextrins	784:812	the fluorinated cellodextrins	784:812	The hybrid architectures obtained depend on the content of fluorine atoms in the fluorinated cellodextrins.
33183596	5	0	theme	certain	703:709	arg1	level					711:715	a certain level	701:715	a certain level of control	701:726	This co-culture approach ensured a certain level of control over the synthesis process.
33183596	8	1	theme	mechanical	993:1002	arg1	analyses					1004:1011	thermal and mechanical analyses	981:1011	thermal and mechanical analyses	981:1011	BC/PHBs were characterized by thermal and mechanical analyses and exhibited a 2.6 times higher capacity for toxic copper adsorption than pure BC.
33183596	2	2	theme	mechanical	377:386	arg1	properties					388:397	enhanced mechanical properties	368:397	enhanced mechanical properties	368:397	BC and PHB can be combined into a composite with enhanced mechanical properties.
33183596	7	3	theme	fibers	918:923	arg1	networks					903:910	networks	903:910	networks of BC fibers connecting PHB particles	903:948	The fabricated composites were networks of BC fibers connecting PHB particles.
33183596	7	3	theme	fibers	918:923	arg1	composites					887:896	The fabricated composites	872:896	The fabricated composites	872:896	The fabricated composites were networks of BC fibers connecting PHB particles.
33183596	4	4	theme	facile	548:553	arg1	xylinus					573:579	a facile Gluconacetobacter xylinus	546:579	a facile Gluconacetobacter xylinus	546:579	Here, BC/PHB composites were synthesized by a facile Gluconacetobacter xylinus and Ralstonia eutropha co-culture method generating BC and PHB simultaneously in situ.
33183596	8	5	theme	toxic	1059:1063	arg1	adsorption					1072:1081	toxic copper adsorption	1059:1081	toxic copper adsorption	1059:1081	BC/PHBs were characterized by thermal and mechanical analyses and exhibited a 2.6 times higher capacity for toxic copper adsorption than pure BC.
33183596	2	6	theme	enhanced	368:375	arg1	properties					388:397	enhanced mechanical properties	368:397	enhanced mechanical properties	368:397	BC and PHB can be combined into a composite with enhanced mechanical properties.
33183596	8	7	theme	copper	1065:1070	arg1	adsorption					1072:1081	toxic copper adsorption	1059:1081	toxic copper adsorption	1059:1081	BC/PHBs were characterized by thermal and mechanical analyses and exhibited a 2.6 times higher capacity for toxic copper adsorption than pure BC.
33183596	1	8	theme	fossil	294:299	arg1	derivatives					306:316	fossil fuel derivatives	294:316	fossil fuel derivatives	294:316	Bacterial cellulose (BC) and polyhydroxybutyrate (PHB) are microbial polymers considered to be promising biodegradable alternatives to fossil fuel derivatives.
33183596	0	9	theme	Gluconacetobacter	109:125	arg1	xylinus					127:133	Gluconacetobacter xylinus	109:133	Gluconacetobacter xylinus	109:133	The facile and controllable synthesis of a bacterial cellulose/polyhydroxybutyrate composite by co-culturing Gluconacetobacter xylinus and Ralstonia eutropha.
33183596	4	10	theme	BC/PHB	508:513	arg1	composites					515:524	BC/PHB composites	508:524	BC/PHB composites	508:524	Here, BC/PHB composites were synthesized by a facile Gluconacetobacter xylinus and Ralstonia eutropha co-culture method generating BC and PHB simultaneously in situ.
33183596	5	11	theme	control	720:726	arg1	level					711:715	a certain level	701:715	a certain level of control	701:726	This co-culture approach ensured a certain level of control over the synthesis process.
33183596	9	12	theme	co-culture	1101:1110	arg1	method					1159:1164	a simple synthesis method	1140:1164	a simple synthesis method to obtain BC/PHB with adjustable characteristics	1140:1213	The co-culture technique described here is a simple synthesis method to obtain BC/PHB with adjustable characteristics.
33183596	9	12	theme	co-culture	1101:1110	arg1	technique					1112:1120	The co-culture technique	1097:1120	The co-culture technique described here	1097:1135	The co-culture technique described here is a simple synthesis method to obtain BC/PHB with adjustable characteristics.
33183596	1	13	theme	fuel	301:304	arg1	derivatives					306:316	fossil fuel derivatives	294:316	fossil fuel derivatives	294:316	Bacterial cellulose (BC) and polyhydroxybutyrate (PHB) are microbial polymers considered to be promising biodegradable alternatives to fossil fuel derivatives.
33183596	0	14	theme	Ralstonia	139:147	arg1	eutropha					149:156	Ralstonia eutropha	139:156	Ralstonia eutropha	139:156	The facile and controllable synthesis of a bacterial cellulose/polyhydroxybutyrate composite by co-culturing Gluconacetobacter xylinus and Ralstonia eutropha.
33183596	5	15	theme	co-culture	673:682	arg1	approach					684:691	This co-culture approach	668:691	This co-culture approach	668:691	This co-culture approach ensured a certain level of control over the synthesis process.
33183596	8	16	theme	pure	1088:1091	arg1	BC					1093:1094	pure BC	1088:1094	pure BC	1088:1094	BC/PHBs were characterized by thermal and mechanical analyses and exhibited a 2.6 times higher capacity for toxic copper adsorption than pure BC.
33183596	8	17	theme	higher	1039:1044	arg1	capacity					1046:1053	a 2.6 times higher capacity	1027:1053	a 2.6 times higher capacity for toxic copper adsorption	1027:1081	BC/PHBs were characterized by thermal and mechanical analyses and exhibited a 2.6 times higher capacity for toxic copper adsorption than pure BC.
33183596	0	18	theme	facile	4:9	arg1	synthesis					28:36	The facile and controllable synthesis	0:36	The facile and controllable synthesis of a bacterial cellulose/polyhydroxybutyrate	0:81	The facile and controllable synthesis of a bacterial cellulose/polyhydroxybutyrate composite by co-culturing Gluconacetobacter xylinus and Ralstonia eutropha.
33183596	6	19	dep	42.88	863:867	arg1	to					860:861	to	860:861	to	860:861	By simply varying the R. eutropha inoculum, the weight ratio of PHB into BC/PHB was adjusted from 15.62 to 42.88 %.
33183596	7	20	theme	fabricated	876:885	arg1	networks					903:910	networks	903:910	networks of BC fibers connecting PHB particles	903:948	The fabricated composites were networks of BC fibers connecting PHB particles.
33183596	7	20	theme	fabricated	876:885	arg1	composites					887:896	The fabricated composites	872:896	The fabricated composites	872:896	The fabricated composites were networks of BC fibers connecting PHB particles.
33183596	4	21	dep	xylinus	573:579	arg1	method					615:620	co-culture method	604:620	co-culture method	604:620	Here, BC/PHB composites were synthesized by a facile Gluconacetobacter xylinus and Ralstonia eutropha co-culture method generating BC and PHB simultaneously in situ.
33183596	4	22	theme	co-culture	604:613	arg1	method					615:620	co-culture method	604:620	co-culture method	604:620	Here, BC/PHB composites were synthesized by a facile Gluconacetobacter xylinus and Ralstonia eutropha co-culture method generating BC and PHB simultaneously in situ.
33183596	6	23	theme	PHB	820:822	arg1	ratio					811:815	the weight ratio	800:815	the weight ratio of PHB into BC/PHB	800:834	By simply varying the R. eutropha inoculum, the weight ratio of PHB into BC/PHB was adjusted from 15.62 to 42.88 %.
33183596	0	24	theme	controllable	15:26	arg1	synthesis					28:36	The facile and controllable synthesis	0:36	The facile and controllable synthesis of a bacterial cellulose/polyhydroxybutyrate	0:81	The facile and controllable synthesis of a bacterial cellulose/polyhydroxybutyrate composite by co-culturing Gluconacetobacter xylinus and Ralstonia eutropha.
33183596	3	25	theme	synthesis	404:412	arg1	processes					414:422	The synthesis processes	400:422	The synthesis processes of BC/PHB composites described until now	400:463	The synthesis processes of BC/PHB composites described until now are complicated with multiple steps.
33183596	9	26	theme	synthesis	1149:1157	arg1	method					1159:1164	a simple synthesis method	1140:1164	a simple synthesis method to obtain BC/PHB with adjustable characteristics	1140:1213	The co-culture technique described here is a simple synthesis method to obtain BC/PHB with adjustable characteristics.
33183596	9	26	theme	synthesis	1149:1157	arg1	technique					1112:1120	The co-culture technique	1097:1120	The co-culture technique described here	1097:1135	The co-culture technique described here is a simple synthesis method to obtain BC/PHB with adjustable characteristics.
33183596	9	27	theme	simple	1142:1147	arg1	method					1159:1164	a simple synthesis method	1140:1164	a simple synthesis method to obtain BC/PHB with adjustable characteristics	1140:1213	The co-culture technique described here is a simple synthesis method to obtain BC/PHB with adjustable characteristics.
33183596	9	27	theme	simple	1142:1147	arg1	technique					1112:1120	The co-culture technique	1097:1120	The co-culture technique described here	1097:1135	The co-culture technique described here is a simple synthesis method to obtain BC/PHB with adjustable characteristics.
33183596	1	28	theme	microbial	218:226	arg1	polyhydroxybutyrate					188:206	polyhydroxybutyrate	188:206	polyhydroxybutyrate (PHB)	188:212	Bacterial cellulose (BC) and polyhydroxybutyrate (PHB) are microbial polymers considered to be promising biodegradable alternatives to fossil fuel derivatives.
33183596	1	28	theme	microbial	218:226	arg1	cellulose					169:177	Bacterial cellulose	159:177	Bacterial cellulose (BC)	159:182	Bacterial cellulose (BC) and polyhydroxybutyrate (PHB) are microbial polymers considered to be promising biodegradable alternatives to fossil fuel derivatives.
33183596	1	28	theme	microbial	218:226	arg1	polymers					228:235	microbial polymers	218:235	microbial polymers considered to be promising biodegradable alternatives to fossil fuel derivatives	218:316	Bacterial cellulose (BC) and polyhydroxybutyrate (PHB) are microbial polymers considered to be promising biodegradable alternatives to fossil fuel derivatives.
33183596	0	29	theme	cellulose/polyhydroxybutyrate	53:81	arg1	synthesis					28:36	The facile and controllable synthesis	0:36	The facile and controllable synthesis of a bacterial cellulose/polyhydroxybutyrate	0:81	The facile and controllable synthesis of a bacterial cellulose/polyhydroxybutyrate composite by co-culturing Gluconacetobacter xylinus and Ralstonia eutropha.
33183596	8	30	theme	thermal	981:987	arg1	analyses					1004:1011	thermal and mechanical analyses	981:1011	thermal and mechanical analyses	981:1011	BC/PHBs were characterized by thermal and mechanical analyses and exhibited a 2.6 times higher capacity for toxic copper adsorption than pure BC.
33183596	3	31	theme	multiple	486:493	arg1	steps					495:499	multiple steps	486:499	multiple steps	486:499	The synthesis processes of BC/PHB composites described until now are complicated with multiple steps.
33183596	9	32	with	BC/PHB	1176:1181	arg1	characteristics					1199:1213	adjustable characteristics	1188:1213	adjustable characteristics	1188:1213	The co-culture technique described here is a simple synthesis method to obtain BC/PHB with adjustable characteristics.
33183596	9	33	theme	adjustable	1188:1197	arg1	characteristics					1199:1213	adjustable characteristics	1188:1213	adjustable characteristics	1188:1213	The co-culture technique described here is a simple synthesis method to obtain BC/PHB with adjustable characteristics.
33183596	3	34	theme	BC/PHB	427:432	arg1	composites					434:443	BC/PHB composites	427:443	BC/PHB composites described until now	427:463	The synthesis processes of BC/PHB composites described until now are complicated with multiple steps.
33183596	4	35	theme	Gluconacetobacter	555:571	arg1	xylinus					573:579	a facile Gluconacetobacter xylinus	546:579	a facile Gluconacetobacter xylinus	546:579	Here, BC/PHB composites were synthesized by a facile Gluconacetobacter xylinus and Ralstonia eutropha co-culture method generating BC and PHB simultaneously in situ.
33183596	2	36	with	composite	353:361	arg1	properties					388:397	enhanced mechanical properties	368:397	enhanced mechanical properties	368:397	BC and PHB can be combined into a composite with enhanced mechanical properties.
33183596	6	37	theme	weight	804:809	arg1	ratio					811:815	the weight ratio	800:815	the weight ratio of PHB into BC/PHB	800:834	By simply varying the R. eutropha inoculum, the weight ratio of PHB into BC/PHB was adjusted from 15.62 to 42.88 %.
33183596	3	38	theme	composites	434:443	arg1	processes					414:422	The synthesis processes	400:422	The synthesis processes of BC/PHB composites described until now	400:463	The synthesis processes of BC/PHB composites described until now are complicated with multiple steps.
33183596	4	39	theme	Ralstonia	585:593	arg1	eutropha					595:602	Ralstonia eutropha	585:602	Ralstonia eutropha	585:602	Here, BC/PHB composites were synthesized by a facile Gluconacetobacter xylinus and Ralstonia eutropha co-culture method generating BC and PHB simultaneously in situ.
33183596	5	40	theme	synthesis	737:745	arg1	process					747:753	the synthesis process	733:753	the synthesis process	733:753	This co-culture approach ensured a certain level of control over the synthesis process.
33183596	1	41	theme	Bacterial	159:167	arg1	BC					180:181	BC	180:181	BC	180:181	Bacterial cellulose (BC) and polyhydroxybutyrate (PHB) are microbial polymers considered to be promising biodegradable alternatives to fossil fuel derivatives.
33183596	1	41	theme	Bacterial	159:167	arg1	polyhydroxybutyrate					188:206	polyhydroxybutyrate	188:206	polyhydroxybutyrate (PHB)	188:212	Bacterial cellulose (BC) and polyhydroxybutyrate (PHB) are microbial polymers considered to be promising biodegradable alternatives to fossil fuel derivatives.
33183596	1	41	theme	Bacterial	159:167	arg1	cellulose					169:177	Bacterial cellulose	159:177	Bacterial cellulose (BC)	159:182	Bacterial cellulose (BC) and polyhydroxybutyrate (PHB) are microbial polymers considered to be promising biodegradable alternatives to fossil fuel derivatives.
33183596	1	41	theme	Bacterial	159:167	arg1	polymers					228:235	microbial polymers	218:235	microbial polymers considered to be promising biodegradable alternatives to fossil fuel derivatives	218:316	Bacterial cellulose (BC) and polyhydroxybutyrate (PHB) are microbial polymers considered to be promising biodegradable alternatives to fossil fuel derivatives.
33183596	4	42	theme	in	659:660	arg1	BC					633:634	BC	633:634	BC	633:634	Here, BC/PHB composites were synthesized by a facile Gluconacetobacter xylinus and Ralstonia eutropha co-culture method generating BC and PHB simultaneously in situ.
33183596	4	43	dep	in	659:660	arg1	situ					662:665	situ	662:665	situ	662:665	Here, BC/PHB composites were synthesized by a facile Gluconacetobacter xylinus and Ralstonia eutropha co-culture method generating BC and PHB simultaneously in situ.
33183596	6	44	theme	eutropha	781:788	arg1	inoculum					790:797	the R. eutropha inoculum	774:797	the R. eutropha inoculum	774:797	By simply varying the R. eutropha inoculum, the weight ratio of PHB into BC/PHB was adjusted from 15.62 to 42.88 %.
33183596	7	45	theme	PHB	936:938	arg1	particles					940:948	PHB particles	936:948	PHB particles	936:948	The fabricated composites were networks of BC fibers connecting PHB particles.
33183596	1	46	theme	promising	254:262	arg1	alternatives					278:289	promising biodegradable alternatives	254:289	promising biodegradable alternatives to fossil fuel derivatives	254:316	Bacterial cellulose (BC) and polyhydroxybutyrate (PHB) are microbial polymers considered to be promising biodegradable alternatives to fossil fuel derivatives.
33183596	0	47	theme	bacterial	43:51	arg1	cellulose/polyhydroxybutyrate					53:81	a bacterial cellulose/polyhydroxybutyrate	41:81	a bacterial cellulose/polyhydroxybutyrate	41:81	The facile and controllable synthesis of a bacterial cellulose/polyhydroxybutyrate composite by co-culturing Gluconacetobacter xylinus and Ralstonia eutropha.
33183596	6	48	theme	R.	778:779	arg1	inoculum					790:797	the R. eutropha inoculum	774:797	the R. eutropha inoculum	774:797	By simply varying the R. eutropha inoculum, the weight ratio of PHB into BC/PHB was adjusted from 15.62 to 42.88 %.
33183596	7	49	theme	BC	915:916	arg1	fibers					918:923	BC fibers	915:923	BC fibers connecting PHB particles	915:948	The fabricated composites were networks of BC fibers connecting PHB particles.
33183596	1	50	theme	biodegradable	264:276	arg1	alternatives					278:289	promising biodegradable alternatives	254:289	promising biodegradable alternatives to fossil fuel derivatives	254:316	Bacterial cellulose (BC) and polyhydroxybutyrate (PHB) are microbial polymers considered to be promising biodegradable alternatives to fossil fuel derivatives.
34619278	7	0	theme	efficient	1199:1207	arg1	strategy					1209:1216	an efficient strategy	1196:1216	an efficient strategy for the production of polysaccharides	1196:1254	This study proposes an efficient strategy for the production of polysaccharides through submerged fermentation of S. commune, and elucidates the detailed mechanism of using Tween 80 as a fermentation stimulatory reagent.
34619278	1	1	with	biomacromolecules	173:189	arg1	activities					216:225	multiple biological activities	196:225	multiple biological activities	196:225	Schizophyllum commune (S. commune) polysaccharides are biomacromolecules with multiple biological activities and wide applications.
34619278	1	1	with	biomacromolecules	173:189	arg1	applications					236:247	wide applications	231:247	wide applications	231:247	Schizophyllum commune (S. commune) polysaccharides are biomacromolecules with multiple biological activities and wide applications.
34619278	0	2	from	Effect	0:5	arg1	production					29:38	the production	25:38	the production of polysaccharides from Schizophyllum commune through submerged fermentation	25:115	Effect of surfactants on the production of polysaccharides from Schizophyllum commune through submerged fermentation.
34619278	2	3	theme	commune	328:334	arg1	fermentation					309:320	submerged fermentation	299:320	submerged fermentation of S. commune using different surfactants	299:362	In this study, polysaccharide production through submerged fermentation of S. commune using different surfactants was investigated.
34619278	5	4	theme	genes	956:960	arg1	levels					905:910	the transcription levels	887:910	the transcription levels of phosphoglucomutase and β-glucan synthase genes in the polysaccharide synthesis pathway	887:1000	Notably, the addition of Tween 80 significantly increased the ATP levels and the transcription levels of phosphoglucomutase and β-glucan synthase genes in the polysaccharide synthesis pathway.
34619278	5	4	theme	genes	956:960	arg1	levels					876:881	the ATP levels	868:881	the ATP levels	868:881	Notably, the addition of Tween 80 significantly increased the ATP levels and the transcription levels of phosphoglucomutase and β-glucan synthase genes in the polysaccharide synthesis pathway.
34619278	3	5	theme	polyoxyethylene	407:421	arg1	Tween					444:448	Tween	444:448	Tween	444:448	The addition of 1 g/L of polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation showed the best promotional effects on collective exopolysaccharide (EPS) production (which increased by 37.17%) while shortening the production cycle by 2 days.
34619278	3	5	theme	polyoxyethylene	407:421	arg1	monooleate					432:441	polyoxyethylene sorbitan monooleate	407:441	polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation	407:489	The addition of 1 g/L of polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation showed the best promotional effects on collective exopolysaccharide (EPS) production (which increased by 37.17%) while shortening the production cycle by 2 days.
34619278	4	6	theme	monosaccharide	657:670	arg1	composition					672:682	The monosaccharide composition	653:682	The monosaccharide composition of the EPS produced when the added Tween 80	653:726	The monosaccharide composition of the EPS produced when the added Tween 80 was similar to that of the control; however, the molecular weight (Mw) was lower.
34619278	4	6	theme	monosaccharide	657:670	arg1	similar					732:738	similar	732:738	similar	732:738	The monosaccharide composition of the EPS produced when the added Tween 80 was similar to that of the control; however, the molecular weight (Mw) was lower.
34619278	6	7	theme	mycelial	1160:1167	arg1	cells					1169:1173	the mycelial cells	1156:1173	the mycelial cells	1156:1173	The addition of Tween 80 reduced the pellet size of the mycelium compared to that of the control, but did not significantly change the microstructure of the mycelial cells.
34619278	2	8	theme	S.	325:326	arg1	commune					328:334	S. commune	325:334	S. commune using different surfactants	325:362	In this study, polysaccharide production through submerged fermentation of S. commune using different surfactants was investigated.
34619278	7	9	theme	submerged	1264:1272	arg1	fermentation					1274:1285	submerged fermentation	1264:1285	submerged fermentation of S. commune	1264:1299	This study proposes an efficient strategy for the production of polysaccharides through submerged fermentation of S. commune, and elucidates the detailed mechanism of using Tween 80 as a fermentation stimulatory reagent.
34619278	3	10	theme	collective	530:539	arg1	EPS					560:562	EPS	560:562	EPS	560:562	The addition of 1 g/L of polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation showed the best promotional effects on collective exopolysaccharide (EPS) production (which increased by 37.17%) while shortening the production cycle by 2 days.
34619278	3	10	theme	collective	530:539	arg1	exopolysaccharide					541:557	collective exopolysaccharide	530:557	collective exopolysaccharide (EPS) production (which increased by 37.17%)	530:602	The addition of 1 g/L of polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation showed the best promotional effects on collective exopolysaccharide (EPS) production (which increased by 37.17%) while shortening the production cycle by 2 days.
34619278	3	11	theme	promotional	507:517	arg1	effects					519:525	the best promotional effects	498:525	the best promotional effects on collective exopolysaccharide (EPS) production (which increased by 37.17%)	498:602	The addition of 1 g/L of polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation showed the best promotional effects on collective exopolysaccharide (EPS) production (which increased by 37.17%) while shortening the production cycle by 2 days.
34619278	5	12	theme	polysaccharide	969:982	arg1	pathway					994:1000	the polysaccharide synthesis pathway	965:1000	the polysaccharide synthesis pathway	965:1000	Notably, the addition of Tween 80 significantly increased the ATP levels and the transcription levels of phosphoglucomutase and β-glucan synthase genes in the polysaccharide synthesis pathway.
34619278	6	13	theme	mycelium	1059:1066	arg1	size					1047:1050	the pellet size	1036:1050	the pellet size of the mycelium	1036:1066	The addition of Tween 80 reduced the pellet size of the mycelium compared to that of the control, but did not significantly change the microstructure of the mycelial cells.
34619278	7	14	theme	detailed	1321:1328	arg1	mechanism					1330:1338	the detailed mechanism	1317:1338	the detailed mechanism of using Tween 80 as a fermentation stimulatory reagent	1317:1394	This study proposes an efficient strategy for the production of polysaccharides through submerged fermentation of S. commune, and elucidates the detailed mechanism of using Tween 80 as a fermentation stimulatory reagent.
34619278	3	15	theme	1 g/L	398:402	arg1	addition					386:393	The addition	382:393	The addition of 1 g/L of polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation	382:489	The addition of 1 g/L of polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation showed the best promotional effects on collective exopolysaccharide (EPS) production (which increased by 37.17%) while shortening the production cycle by 2 days.
34619278	3	16	from	effects	519:525	arg1	production					565:574	collective exopolysaccharide (EPS) production	530:574	collective exopolysaccharide (EPS) production (which increased by 37.17%)	530:602	The addition of 1 g/L of polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation showed the best promotional effects on collective exopolysaccharide (EPS) production (which increased by 37.17%) while shortening the production cycle by 2 days.
34619278	4	17	theme	molecular	777:785	arg1	Mw					795:796	Mw	795:796	Mw	795:796	The monosaccharide composition of the EPS produced when the added Tween 80 was similar to that of the control; however, the molecular weight (Mw) was lower.
34619278	4	17	theme	molecular	777:785	arg1	lower					803:807	lower	803:807	lower	803:807	The monosaccharide composition of the EPS produced when the added Tween 80 was similar to that of the control; however, the molecular weight (Mw) was lower.
34619278	4	17	theme	molecular	777:785	arg1	weight					787:792	the molecular weight	773:792	the molecular weight (Mw)	773:797	The monosaccharide composition of the EPS produced when the added Tween 80 was similar to that of the control; however, the molecular weight (Mw) was lower.
34619278	1	18	theme	multiple	196:203	arg1	activities					216:225	multiple biological activities	196:225	multiple biological activities	196:225	Schizophyllum commune (S. commune) polysaccharides are biomacromolecules with multiple biological activities and wide applications.
34619278	7	19	theme	S.	1290:1291	arg1	commune					1293:1299	S. commune	1290:1299	S. commune	1290:1299	This study proposes an efficient strategy for the production of polysaccharides through submerged fermentation of S. commune, and elucidates the detailed mechanism of using Tween 80 as a fermentation stimulatory reagent.
34619278	4	20	theme	EPS	691:693	arg1	composition					672:682	The monosaccharide composition	653:682	The monosaccharide composition of the EPS produced when the added Tween 80	653:726	The monosaccharide composition of the EPS produced when the added Tween 80 was similar to that of the control; however, the molecular weight (Mw) was lower.
34619278	4	20	theme	EPS	691:693	arg1	similar					732:738	similar	732:738	similar	732:738	The monosaccharide composition of the EPS produced when the added Tween 80 was similar to that of the control; however, the molecular weight (Mw) was lower.
34619278	1	21	theme	biological	205:214	arg1	activities					216:225	multiple biological activities	196:225	multiple biological activities	196:225	Schizophyllum commune (S. commune) polysaccharides are biomacromolecules with multiple biological activities and wide applications.
34619278	5	22	theme	β-glucan	938:945	arg1	genes					956:960	β-glucan synthase genes	938:960	β-glucan synthase genes	938:960	Notably, the addition of Tween 80 significantly increased the ATP levels and the transcription levels of phosphoglucomutase and β-glucan synthase genes in the polysaccharide synthesis pathway.
34619278	3	23	from	1 g/L	398:402	arg1	beginning					461:469	the beginning	457:469	the beginning of the fermentation	457:489	The addition of 1 g/L of polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation showed the best promotional effects on collective exopolysaccharide (EPS) production (which increased by 37.17%) while shortening the production cycle by 2 days.
34619278	3	24	theme	sorbitan	423:430	arg1	Tween					444:448	Tween	444:448	Tween	444:448	The addition of 1 g/L of polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation showed the best promotional effects on collective exopolysaccharide (EPS) production (which increased by 37.17%) while shortening the production cycle by 2 days.
34619278	3	24	theme	sorbitan	423:430	arg1	monooleate					432:441	polyoxyethylene sorbitan monooleate	407:441	polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation	407:489	The addition of 1 g/L of polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation showed the best promotional effects on collective exopolysaccharide (EPS) production (which increased by 37.17%) while shortening the production cycle by 2 days.
34619278	5	25	from	levels	876:881	arg1	pathway					994:1000	the polysaccharide synthesis pathway	965:1000	the polysaccharide synthesis pathway	965:1000	Notably, the addition of Tween 80 significantly increased the ATP levels and the transcription levels of phosphoglucomutase and β-glucan synthase genes in the polysaccharide synthesis pathway.
34619278	2	26	theme	different	342:350	arg1	surfactants					352:362	different surfactants	342:362	different surfactants	342:362	In this study, polysaccharide production through submerged fermentation of S. commune using different surfactants was investigated.
34619278	7	27	theme	fermentation	1363:1374	arg1	Tween					1349:1353	Tween	1349:1353	Tween	1349:1353	This study proposes an efficient strategy for the production of polysaccharides through submerged fermentation of S. commune, and elucidates the detailed mechanism of using Tween 80 as a fermentation stimulatory reagent.
34619278	7	27	theme	fermentation	1363:1374	arg1	reagent					1388:1394	a fermentation stimulatory reagent	1361:1394	a fermentation stimulatory reagent	1361:1394	This study proposes an efficient strategy for the production of polysaccharides through submerged fermentation of S. commune, and elucidates the detailed mechanism of using Tween 80 as a fermentation stimulatory reagent.
34619278	0	28	theme	surfactants	10:20	arg1	Effect					0:5	Effect	0:5	Effect of surfactants on the production of polysaccharides from Schizophyllum commune through submerged fermentation.	0:116	Effect of surfactants on the production of polysaccharides from Schizophyllum commune through submerged fermentation.
34619278	1	29	theme	Schizophyllum	118:130	arg1	commune					132:138	Schizophyllum commune	118:138	Schizophyllum commune (S. commune) polysaccharides	118:167	Schizophyllum commune (S. commune) polysaccharides are biomacromolecules with multiple biological activities and wide applications.
34619278	1	29	theme	Schizophyllum	118:130	arg1	commune					144:150	S. commune	141:150	S. commune	141:150	Schizophyllum commune (S. commune) polysaccharides are biomacromolecules with multiple biological activities and wide applications.
34619278	1	30	theme	wide	231:234	arg1	applications					236:247	wide applications	231:247	wide applications	231:247	Schizophyllum commune (S. commune) polysaccharides are biomacromolecules with multiple biological activities and wide applications.
34619278	3	31	theme	monooleate	432:441	arg1	1 g/L					398:402	1 g/L	398:402	1 g/L of polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation	398:489	The addition of 1 g/L of polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation showed the best promotional effects on collective exopolysaccharide (EPS) production (which increased by 37.17%) while shortening the production cycle by 2 days.
34619278	0	32	from	commune	78:84	arg1	production					29:38	the production	25:38	the production of polysaccharides from Schizophyllum commune through submerged fermentation	25:115	Effect of surfactants on the production of polysaccharides from Schizophyllum commune through submerged fermentation.
34619278	3	33	theme	production	625:634	arg1	cycle					636:640	the production cycle	621:640	the production cycle	621:640	The addition of 1 g/L of polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation showed the best promotional effects on collective exopolysaccharide (EPS) production (which increased by 37.17%) while shortening the production cycle by 2 days.
34619278	6	34	theme	pellet	1040:1045	arg1	size					1047:1050	the pellet size	1036:1050	the pellet size of the mycelium	1036:1066	The addition of Tween 80 reduced the pellet size of the mycelium compared to that of the control, but did not significantly change the microstructure of the mycelial cells.
34619278	7	35	theme	stimulatory	1376:1386	arg1	Tween					1349:1353	Tween	1349:1353	Tween	1349:1353	This study proposes an efficient strategy for the production of polysaccharides through submerged fermentation of S. commune, and elucidates the detailed mechanism of using Tween 80 as a fermentation stimulatory reagent.
34619278	7	35	theme	stimulatory	1376:1386	arg1	reagent					1388:1394	a fermentation stimulatory reagent	1361:1394	a fermentation stimulatory reagent	1361:1394	This study proposes an efficient strategy for the production of polysaccharides through submerged fermentation of S. commune, and elucidates the detailed mechanism of using Tween 80 as a fermentation stimulatory reagent.
34619278	5	36	theme	Tween	835:839	arg1	addition					823:830	the addition	819:830	the addition of Tween 80	819:842	Notably, the addition of Tween 80 significantly increased the ATP levels and the transcription levels of phosphoglucomutase and β-glucan synthase genes in the polysaccharide synthesis pathway.
34619278	0	37	theme	polysaccharides	43:57	arg1	production					29:38	the production	25:38	the production of polysaccharides from Schizophyllum commune through submerged fermentation	25:115	Effect of surfactants on the production of polysaccharides from Schizophyllum commune through submerged fermentation.
34619278	1	38	theme	commune	132:138	arg1	polysaccharides					153:167	Schizophyllum commune (S. commune) polysaccharides	118:167	Schizophyllum commune (S. commune) polysaccharides	118:167	Schizophyllum commune (S. commune) polysaccharides are biomacromolecules with multiple biological activities and wide applications.
34619278	1	38	theme	commune	132:138	arg1	biomacromolecules					173:189	biomacromolecules	173:189	biomacromolecules with multiple biological activities and wide applications	173:247	Schizophyllum commune (S. commune) polysaccharides are biomacromolecules with multiple biological activities and wide applications.
34619278	3	39	theme	exopolysaccharide	541:557	arg1	production					565:574	collective exopolysaccharide (EPS) production	530:574	collective exopolysaccharide (EPS) production (which increased by 37.17%)	530:602	The addition of 1 g/L of polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation showed the best promotional effects on collective exopolysaccharide (EPS) production (which increased by 37.17%) while shortening the production cycle by 2 days.
34619278	5	40	from	levels	905:910	arg1	pathway					994:1000	the polysaccharide synthesis pathway	965:1000	the polysaccharide synthesis pathway	965:1000	Notably, the addition of Tween 80 significantly increased the ATP levels and the transcription levels of phosphoglucomutase and β-glucan synthase genes in the polysaccharide synthesis pathway.
34619278	2	41	theme	submerged	299:307	arg1	fermentation					309:320	submerged fermentation	299:320	submerged fermentation of S. commune using different surfactants	299:362	In this study, polysaccharide production through submerged fermentation of S. commune using different surfactants was investigated.
34619278	1	42	theme	S.	141:142	arg1	commune					132:138	Schizophyllum commune	118:138	Schizophyllum commune (S. commune) polysaccharides	118:167	Schizophyllum commune (S. commune) polysaccharides are biomacromolecules with multiple biological activities and wide applications.
34619278	1	42	theme	S.	141:142	arg1	commune					144:150	S. commune	141:150	S. commune	141:150	Schizophyllum commune (S. commune) polysaccharides are biomacromolecules with multiple biological activities and wide applications.
34619278	5	43	theme	synthase	947:954	arg1	genes					956:960	β-glucan synthase genes	938:960	β-glucan synthase genes	938:960	Notably, the addition of Tween 80 significantly increased the ATP levels and the transcription levels of phosphoglucomutase and β-glucan synthase genes in the polysaccharide synthesis pathway.
34619278	5	44	theme	synthesis	984:992	arg1	pathway					994:1000	the polysaccharide synthesis pathway	965:1000	the polysaccharide synthesis pathway	965:1000	Notably, the addition of Tween 80 significantly increased the ATP levels and the transcription levels of phosphoglucomutase and β-glucan synthase genes in the polysaccharide synthesis pathway.
34619278	5	45	theme	transcription	891:903	arg1	levels					905:910	the transcription levels	887:910	the transcription levels of phosphoglucomutase and β-glucan synthase genes in the polysaccharide synthesis pathway	887:1000	Notably, the addition of Tween 80 significantly increased the ATP levels and the transcription levels of phosphoglucomutase and β-glucan synthase genes in the polysaccharide synthesis pathway.
34619278	6	46	theme	cells	1169:1173	arg1	microstructure					1138:1151	the microstructure	1134:1151	the microstructure of the mycelial cells	1134:1173	The addition of Tween 80 reduced the pellet size of the mycelium compared to that of the control, but did not significantly change the microstructure of the mycelial cells.
34619278	3	47	from	beginning	461:469	arg1	1 g/L					398:402	1 g/L	398:402	1 g/L of polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation	398:489	The addition of 1 g/L of polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation showed the best promotional effects on collective exopolysaccharide (EPS) production (which increased by 37.17%) while shortening the production cycle by 2 days.
34619278	3	47	from	beginning	461:469	arg1	Tween					444:448	Tween	444:448	Tween	444:448	The addition of 1 g/L of polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation showed the best promotional effects on collective exopolysaccharide (EPS) production (which increased by 37.17%) while shortening the production cycle by 2 days.
34619278	3	47	from	beginning	461:469	arg1	monooleate					432:441	polyoxyethylene sorbitan monooleate	407:441	polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation	407:489	The addition of 1 g/L of polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation showed the best promotional effects on collective exopolysaccharide (EPS) production (which increased by 37.17%) while shortening the production cycle by 2 days.
34619278	5	48	theme	phosphoglucomutase	915:932	arg1	levels					905:910	the transcription levels	887:910	the transcription levels of phosphoglucomutase and β-glucan synthase genes in the polysaccharide synthesis pathway	887:1000	Notably, the addition of Tween 80 significantly increased the ATP levels and the transcription levels of phosphoglucomutase and β-glucan synthase genes in the polysaccharide synthesis pathway.
34619278	5	48	theme	phosphoglucomutase	915:932	arg1	levels					876:881	the ATP levels	868:881	the ATP levels	868:881	Notably, the addition of Tween 80 significantly increased the ATP levels and the transcription levels of phosphoglucomutase and β-glucan synthase genes in the polysaccharide synthesis pathway.
34619278	0	49	theme	Schizophyllum	64:76	arg1	commune					78:84	Schizophyllum commune	64:84	Schizophyllum commune	64:84	Effect of surfactants on the production of polysaccharides from Schizophyllum commune through submerged fermentation.
34619278	7	50	theme	commune	1293:1299	arg1	fermentation					1274:1285	submerged fermentation	1264:1285	submerged fermentation of S. commune	1264:1299	This study proposes an efficient strategy for the production of polysaccharides through submerged fermentation of S. commune, and elucidates the detailed mechanism of using Tween 80 as a fermentation stimulatory reagent.
34619278	5	51	theme	ATP	872:874	arg1	levels					876:881	the ATP levels	868:881	the ATP levels	868:881	Notably, the addition of Tween 80 significantly increased the ATP levels and the transcription levels of phosphoglucomutase and β-glucan synthase genes in the polysaccharide synthesis pathway.
34619278	3	52	theme	fermentation	478:489	arg1	beginning					461:469	the beginning	457:469	the beginning of the fermentation	457:489	The addition of 1 g/L of polyoxyethylene sorbitan monooleate (Tween 80) at the beginning of the fermentation showed the best promotional effects on collective exopolysaccharide (EPS) production (which increased by 37.17%) while shortening the production cycle by 2 days.
34619278	6	53	theme	Tween	1019:1023	arg1	addition					1007:1014	The addition	1003:1014	The addition of Tween 80	1003:1026	The addition of Tween 80 reduced the pellet size of the mycelium compared to that of the control, but did not significantly change the microstructure of the mycelial cells.
34619278	2	54	theme	polysaccharide	265:278	arg1	production					280:289	polysaccharide production	265:289	polysaccharide production through submerged fermentation of S. commune using different surfactants	265:362	In this study, polysaccharide production through submerged fermentation of S. commune using different surfactants was investigated.
34619278	7	55	theme	polysaccharides	1240:1254	arg1	production					1226:1235	the production	1222:1235	the production of polysaccharides	1222:1254	This study proposes an efficient strategy for the production of polysaccharides through submerged fermentation of S. commune, and elucidates the detailed mechanism of using Tween 80 as a fermentation stimulatory reagent.
34619278	0	56	theme	submerged	94:102	arg1	fermentation					104:115	submerged fermentation	94:115	submerged fermentation	94:115	Effect of surfactants on the production of polysaccharides from Schizophyllum commune through submerged fermentation.
34008287	0	0	theme	adsorption	79:88	arg1	process					90:96	an adsorption process	76:96	an adsorption process	76:96	Carbon dots crosslinked chitosan/cellulose sponge capture of methyl blue by an adsorption process.
34008287	6	1	theme	adsorption	888:897	arg1	data					908:911	the adsorption isotherm data	884:911	the adsorption isotherm data	884:911	In addition, the pseudo-second-order kinetic model was matched with the adsorption kinetic experimental data, and the adsorption isotherm data can be described by the Langmuir models.
34008287	4	2	theme	tangled	555:561	arg1	units					576:580	tangled fibre bundle units	555:580	tangled fibre bundle units	555:580	The abundant amount of tangled fibre bundle units can offer plentiful active adsorption sites to collect the dye molecules.
34008287	3	3	with	fibre	439:443	arg1	bundle					452:457	a bundle	450:457	a bundle formed by hydrogen bonds between CA-CDs and the CS/MCC composite matrix	450:529	The morphology of the sponge was a tangled fibre with a bundle formed by hydrogen bonds between CA-CDs and the CS/MCC composite matrix.
34008287	7	4	theme	high	1111:1114	arg1	capacity					1127:1134	high adsorption capacity	1111:1134	high adsorption capacity	1111:1134	This study proposed that the CS/MCC/CA-CDs sponge adsorbent creates tremendous potential application value in wastewater treatment due to its fast kinetics, high adsorption capacity, simple preparation, and eco-friendly properties.
34008287	1	5	theme	industrial	158:167	arg1	wastewater					169:178	industrial wastewater	158:178	industrial wastewater	158:178	For environmental protection, organic dyes and solvents of industrial wastewater must be eliminated.
34008287	7	6	theme	fast	1096:1099	arg1	kinetics					1101:1108	its fast kinetics	1092:1108	its fast kinetics	1092:1108	This study proposed that the CS/MCC/CA-CDs sponge adsorbent creates tremendous potential application value in wastewater treatment due to its fast kinetics, high adsorption capacity, simple preparation, and eco-friendly properties.
34008287	2	7	theme	CS/MCC/CA-CDs	296:308	arg1	sponge					311:316	crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge	247:316	a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge	206:316	Here, a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge was synthesized to study its adsorption performance for methyl blue (MB) dye.
34008287	2	8	link	crosslinked	247:257	arg1	sponge					311:316	crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge	247:316	a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge	206:316	Here, a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge was synthesized to study its adsorption performance for methyl blue (MB) dye.
34008287	4	9	theme	fibre	563:567	arg1	units					576:580	tangled fibre bundle units	555:580	tangled fibre bundle units	555:580	The abundant amount of tangled fibre bundle units can offer plentiful active adsorption sites to collect the dye molecules.
34008287	2	10	theme	cellulose	285:293	arg1	sponge					311:316	crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge	247:316	a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge	206:316	Here, a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge was synthesized to study its adsorption performance for methyl blue (MB) dye.
34008287	1	11	theme	wastewater	169:178	arg1	solvents					146:153	solvents	146:153	solvents of industrial wastewater	146:178	For environmental protection, organic dyes and solvents of industrial wastewater must be eliminated.
34008287	1	11	theme	wastewater	169:178	arg1	dyes					137:140	organic dyes	129:140	organic dyes	129:140	For environmental protection, organic dyes and solvents of industrial wastewater must be eliminated.
34008287	2	12	dep	CA-CDs	239:244	arg1	sponge					311:316	crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge	247:316	a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge	206:316	Here, a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge was synthesized to study its adsorption performance for methyl blue (MB) dye.
34008287	6	13	theme	experimental	861:872	arg1	data					874:877	kinetic experimental data	853:877	kinetic experimental data	853:877	In addition, the pseudo-second-order kinetic model was matched with the adsorption kinetic experimental data, and the adsorption isotherm data can be described by the Langmuir models.
34008287	7	14	theme	wastewater	1064:1073	arg1	treatment					1075:1083	wastewater treatment	1064:1083	wastewater treatment due to its fast kinetics, high adsorption capacity, simple preparation, and eco-friendly properties	1064:1183	This study proposed that the CS/MCC/CA-CDs sponge adsorbent creates tremendous potential application value in wastewater treatment due to its fast kinetics, high adsorption capacity, simple preparation, and eco-friendly properties.
34008287	2	15	theme	chitosan/microcrystalline	259:283	arg1	sponge					311:316	crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge	247:316	a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge	206:316	Here, a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge was synthesized to study its adsorption performance for methyl blue (MB) dye.
34008287	6	16	theme	kinetic	853:859	arg1	data					874:877	kinetic experimental data	853:877	kinetic experimental data	853:877	In addition, the pseudo-second-order kinetic model was matched with the adsorption kinetic experimental data, and the adsorption isotherm data can be described by the Langmuir models.
34008287	0	17	theme	Carbon	0:5	arg1	dots					7:10	Carbon dots	0:10	Carbon dots	0:10	Carbon dots crosslinked chitosan/cellulose sponge capture of methyl blue by an adsorption process.
34008287	7	18	theme	simple	1137:1142	arg1	preparation					1144:1154	simple preparation	1137:1154	simple preparation	1137:1154	This study proposed that the CS/MCC/CA-CDs sponge adsorbent creates tremendous potential application value in wastewater treatment due to its fast kinetics, high adsorption capacity, simple preparation, and eco-friendly properties.
34008287	3	19	theme	sponge	418:423	arg1	fibre					439:443	fibre	439:443	fibre	439:443	The morphology of the sponge was a tangled fibre with a bundle formed by hydrogen bonds between CA-CDs and the CS/MCC composite matrix.
34008287	3	19	theme	sponge	418:423	arg1	morphology					400:409	The morphology	396:409	The morphology of the sponge	396:423	The morphology of the sponge was a tangled fibre with a bundle formed by hydrogen bonds between CA-CDs and the CS/MCC composite matrix.
34008287	0	20	theme	chitosan/cellulose	24:41	arg1	capture					50:56	chitosan/cellulose sponge capture	24:56	chitosan/cellulose sponge capture of methyl blue	24:71	Carbon dots crosslinked chitosan/cellulose sponge capture of methyl blue by an adsorption process.
34008287	6	21	theme	Langmuir	937:944	arg1	models					946:951	the Langmuir models	933:951	the Langmuir models	933:951	In addition, the pseudo-second-order kinetic model was matched with the adsorption kinetic experimental data, and the adsorption isotherm data can be described by the Langmuir models.
34008287	5	22	theme	298 K	763:767	arg1	pH 10					736:740	pH 10	736:740	pH 10	736:740	The adsorption capacity of the CS/MCC/CA-CDs sponge toward MB was 306.8 mg/g at pH 10 and a temperature of 298 K.
34008287	5	22	theme	298 K	763:767	arg1	temperature					748:758	a temperature	746:758	a temperature of 298 K	746:767	The adsorption capacity of the CS/MCC/CA-CDs sponge toward MB was 306.8 mg/g at pH 10 and a temperature of 298 K.
34008287	4	23	theme	adsorption	609:618	arg1	sites					620:624	plentiful active adsorption sites	592:624	plentiful active adsorption sites	592:624	The abundant amount of tangled fibre bundle units can offer plentiful active adsorption sites to collect the dye molecules.
34008287	7	24	theme	application	1043:1053	arg1	value					1055:1059	tremendous potential application value	1022:1059	tremendous potential application value	1022:1059	This study proposed that the CS/MCC/CA-CDs sponge adsorbent creates tremendous potential application value in wastewater treatment due to its fast kinetics, high adsorption capacity, simple preparation, and eco-friendly properties.
34008287	2	25	dep	blue	381:384	arg1	MB					387:388	MB	387:388	MB	387:388	Here, a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge was synthesized to study its adsorption performance for methyl blue (MB) dye.
34008287	1	26	theme	environmental	103:115	arg1	protection					117:126	environmental protection	103:126	environmental protection	103:126	For environmental protection, organic dyes and solvents of industrial wastewater must be eliminated.
34008287	4	27	theme	active	602:607	arg1	sites					620:624	plentiful active adsorption sites	592:624	plentiful active adsorption sites	592:624	The abundant amount of tangled fibre bundle units can offer plentiful active adsorption sites to collect the dye molecules.
34008287	4	28	theme	abundant	536:543	arg1	units					576:580	tangled fibre bundle units	555:580	tangled fibre bundle units	555:580	The abundant amount of tangled fibre bundle units can offer plentiful active adsorption sites to collect the dye molecules.
34008287	4	28	theme	abundant	536:543	arg1	amount					545:550	The abundant amount	532:550	The abundant amount of tangled fibre bundle units	532:580	The abundant amount of tangled fibre bundle units can offer plentiful active adsorption sites to collect the dye molecules.
34008287	5	29	theme	CS/MCC/CA-CDs	687:699	arg1	sponge					701:706	the CS/MCC/CA-CDs sponge	683:706	the CS/MCC/CA-CDs sponge	683:706	The adsorption capacity of the CS/MCC/CA-CDs sponge toward MB was 306.8 mg/g at pH 10 and a temperature of 298 K.
34008287	7	30	theme	eco-friendly	1161:1172	arg1	properties					1174:1183	eco-friendly properties	1161:1183	eco-friendly properties	1161:1183	This study proposed that the CS/MCC/CA-CDs sponge adsorbent creates tremendous potential application value in wastewater treatment due to its fast kinetics, high adsorption capacity, simple preparation, and eco-friendly properties.
34008287	2	31	theme	dots	233:236	arg1	CA-CDs					239:244	a citric acid-based carbon dots (CA-CDs)	206:245	a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge	206:316	Here, a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge was synthesized to study its adsorption performance for methyl blue (MB) dye.
34008287	4	32	theme	dye	641:643	arg1	molecules					645:653	the dye molecules	637:653	the dye molecules	637:653	The abundant amount of tangled fibre bundle units can offer plentiful active adsorption sites to collect the dye molecules.
34008287	7	33	theme	adsorption	1116:1125	arg1	capacity					1127:1134	high adsorption capacity	1111:1134	high adsorption capacity	1111:1134	This study proposed that the CS/MCC/CA-CDs sponge adsorbent creates tremendous potential application value in wastewater treatment due to its fast kinetics, high adsorption capacity, simple preparation, and eco-friendly properties.
34008287	0	34	theme	sponge	43:48	arg1	capture					50:56	chitosan/cellulose sponge capture	24:56	chitosan/cellulose sponge capture of methyl blue	24:71	Carbon dots crosslinked chitosan/cellulose sponge capture of methyl blue by an adsorption process.
34008287	5	35	theme	sponge	701:706	arg1	capacity					671:678	The adsorption capacity	656:678	The adsorption capacity of the CS/MCC/CA-CDs sponge toward MB	656:716	The adsorption capacity of the CS/MCC/CA-CDs sponge toward MB was 306.8 mg/g at pH 10 and a temperature of 298 K.
34008287	2	36	theme	carbon	226:231	arg1	CA-CDs					239:244	a citric acid-based carbon dots (CA-CDs)	206:245	a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge	206:316	Here, a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge was synthesized to study its adsorption performance for methyl blue (MB) dye.
34008287	7	37	theme	CS/MCC/CA-CDs	983:995	arg1	sponge					997:1002	the CS/MCC/CA-CDs sponge adsorbent	979:1012	the CS/MCC/CA-CDs sponge adsorbent	979:1012	This study proposed that the CS/MCC/CA-CDs sponge adsorbent creates tremendous potential application value in wastewater treatment due to its fast kinetics, high adsorption capacity, simple preparation, and eco-friendly properties.
34008287	0	38	theme	methyl	61:66	arg1	capture					50:56	chitosan/cellulose sponge capture	24:56	chitosan/cellulose sponge capture of methyl blue	24:71	Carbon dots crosslinked chitosan/cellulose sponge capture of methyl blue by an adsorption process.
34008287	7	39	theme	tremendous	1022:1031	arg1	value					1055:1059	tremendous potential application value	1022:1059	tremendous potential application value	1022:1059	This study proposed that the CS/MCC/CA-CDs sponge adsorbent creates tremendous potential application value in wastewater treatment due to its fast kinetics, high adsorption capacity, simple preparation, and eco-friendly properties.
34008287	2	40	theme	acid-based	215:224	arg1	CA-CDs					239:244	a citric acid-based carbon dots (CA-CDs)	206:245	a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge	206:316	Here, a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge was synthesized to study its adsorption performance for methyl blue (MB) dye.
34008287	7	41	theme	adsorbent	1004:1012	arg1	sponge					997:1002	the CS/MCC/CA-CDs sponge adsorbent	979:1012	the CS/MCC/CA-CDs sponge adsorbent	979:1012	This study proposed that the CS/MCC/CA-CDs sponge adsorbent creates tremendous potential application value in wastewater treatment due to its fast kinetics, high adsorption capacity, simple preparation, and eco-friendly properties.
34008287	2	42	theme	adsorption	347:356	arg1	performance					358:368	its adsorption performance	343:368	its adsorption performance for methyl blue (MB) dye	343:393	Here, a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge was synthesized to study its adsorption performance for methyl blue (MB) dye.
34008287	6	43	dep	adsorption	842:851	arg1	described					920:928	described	920:928	can be described by the Langmuir models	913:951	In addition, the pseudo-second-order kinetic model was matched with the adsorption kinetic experimental data, and the adsorption isotherm data can be described by the Langmuir models.
34008287	7	44	theme	potential	1033:1041	arg1	value					1055:1059	tremendous potential application value	1022:1059	tremendous potential application value	1022:1059	This study proposed that the CS/MCC/CA-CDs sponge adsorbent creates tremendous potential application value in wastewater treatment due to its fast kinetics, high adsorption capacity, simple preparation, and eco-friendly properties.
34008287	4	45	theme	bundle	569:574	arg1	units					576:580	tangled fibre bundle units	555:580	tangled fibre bundle units	555:580	The abundant amount of tangled fibre bundle units can offer plentiful active adsorption sites to collect the dye molecules.
34008287	2	46	theme	citric	208:213	arg1	CA-CDs					239:244	a citric acid-based carbon dots (CA-CDs)	206:245	a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge	206:316	Here, a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge was synthesized to study its adsorption performance for methyl blue (MB) dye.
34008287	3	47	theme	hydrogen	469:476	arg1	bonds					478:482	hydrogen bonds	469:482	hydrogen bonds between CA-CDs and the CS/MCC composite matrix	469:529	The morphology of the sponge was a tangled fibre with a bundle formed by hydrogen bonds between CA-CDs and the CS/MCC composite matrix.
34008287	5	48	theme	adsorption	660:669	arg1	capacity					671:678	The adsorption capacity	656:678	The adsorption capacity of the CS/MCC/CA-CDs sponge toward MB	656:716	The adsorption capacity of the CS/MCC/CA-CDs sponge toward MB was 306.8 mg/g at pH 10 and a temperature of 298 K.
34008287	2	49	theme	crosslinked	247:257	arg1	sponge					311:316	crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge	247:316	a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge	206:316	Here, a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge was synthesized to study its adsorption performance for methyl blue (MB) dye.
34008287	1	50	theme	organic	129:135	arg1	dyes					137:140	organic dyes	129:140	organic dyes	129:140	For environmental protection, organic dyes and solvents of industrial wastewater must be eliminated.
34008287	3	51	theme	CS/MCC	507:512	arg1	matrix					524:529	the CS/MCC composite matrix	503:529	the CS/MCC composite matrix	503:529	The morphology of the sponge was a tangled fibre with a bundle formed by hydrogen bonds between CA-CDs and the CS/MCC composite matrix.
34008287	6	52	theme	kinetic	807:813	arg1	model					815:819	the pseudo-second-order kinetic model	783:819	the pseudo-second-order kinetic model	783:819	In addition, the pseudo-second-order kinetic model was matched with the adsorption kinetic experimental data, and the adsorption isotherm data can be described by the Langmuir models.
34008287	3	53	theme	composite	514:522	arg1	matrix					524:529	the CS/MCC composite matrix	503:529	the CS/MCC composite matrix	503:529	The morphology of the sponge was a tangled fibre with a bundle formed by hydrogen bonds between CA-CDs and the CS/MCC composite matrix.
34008287	4	54	theme	units	576:580	arg1	units					576:580	tangled fibre bundle units	555:580	tangled fibre bundle units	555:580	The abundant amount of tangled fibre bundle units can offer plentiful active adsorption sites to collect the dye molecules.
34008287	4	54	theme	units	576:580	arg1	amount					545:550	The abundant amount	532:550	The abundant amount of tangled fibre bundle units	532:580	The abundant amount of tangled fibre bundle units can offer plentiful active adsorption sites to collect the dye molecules.
34008287	6	55	theme	pseudo-second-order	787:805	arg1	model					815:819	the pseudo-second-order kinetic model	783:819	the pseudo-second-order kinetic model	783:819	In addition, the pseudo-second-order kinetic model was matched with the adsorption kinetic experimental data, and the adsorption isotherm data can be described by the Langmuir models.
34008287	2	56	theme	blue	381:384	arg1	dye					391:393	methyl blue (MB) dye	374:393	methyl blue (MB) dye	374:393	Here, a citric acid-based carbon dots (CA-CDs) crosslinked chitosan/microcrystalline cellulose (CS/MCC/CA-CDs) sponge was synthesized to study its adsorption performance for methyl blue (MB) dye.
34008287	4	57	theme	plentiful	592:600	arg1	sites					620:624	plentiful active adsorption sites	592:624	plentiful active adsorption sites	592:624	The abundant amount of tangled fibre bundle units can offer plentiful active adsorption sites to collect the dye molecules.
34008287	0	58	theme	blue	68:71	arg1	methyl					61:66	methyl blue	61:71	methyl blue	61:71	Carbon dots crosslinked chitosan/cellulose sponge capture of methyl blue by an adsorption process.
34008287	6	59	theme	isotherm	899:906	arg1	data					908:911	the adsorption isotherm data	884:911	the adsorption isotherm data	884:911	In addition, the pseudo-second-order kinetic model was matched with the adsorption kinetic experimental data, and the adsorption isotherm data can be described by the Langmuir models.
32408264	5	0	from	Incorporation	963:975	arg1	monolayers					1008:1017	the raft monolayers	999:1017	the raft monolayers	999:1017	This applies to both a commercial chitosan and chitosans soluble at physiological pH. Incorporation of these chitosans in the raft monolayers was confirmed in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments, where both the tail groups and headgroups were found to interact with chitosan.
32408264	0	1	from	effects	18:24	arg1	models					43:48	cell membrane models	29:48	cell membrane models	29:48	Enhanced chitosan effects on cell membrane models made with lipid raft monolayers.
32408264	6	2	theme	membrane	1228:1235	arg1	models					1237:1242	membrane models	1228:1242	membrane models	1228:1242	Since the effects on membrane models may be observed at such small concentrations for chitosans and probably other molecules, some studies may have to be revisited where neat phospholipids should be replaced by lipid raft compositions.
32408264	5	3	theme	tail	1142:1145	arg1	groups					1147:1152	the tail groups	1138:1152	the tail groups	1138:1152	This applies to both a commercial chitosan and chitosans soluble at physiological pH. Incorporation of these chitosans in the raft monolayers was confirmed in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments, where both the tail groups and headgroups were found to interact with chitosan.
32408264	0	4	theme	raft	66:69	arg1	monolayers					71:80	lipid raft monolayers	60:80	lipid raft monolayers	60:80	Enhanced chitosan effects on cell membrane models made with lipid raft monolayers.
32408264	5	5	theme	absorption	1079:1088	arg1	PM-IRRAS					1104:1111	PM-IRRAS	1104:1111	PM-IRRAS	1104:1111	This applies to both a commercial chitosan and chitosans soluble at physiological pH. Incorporation of these chitosans in the raft monolayers was confirmed in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments, where both the tail groups and headgroups were found to interact with chitosan.
32408264	5	5	theme	absorption	1079:1088	arg1	spectroscopy					1090:1101	infrared reflection absorption spectroscopy	1059:1101	polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments	1036:1124	This applies to both a commercial chitosan and chitosans soluble at physiological pH. Incorporation of these chitosans in the raft monolayers was confirmed in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments, where both the tail groups and headgroups were found to interact with chitosan.
32408264	5	6	theme	spectroscopy	1090:1101	arg1	experiments					1114:1124	polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments	1036:1124	polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments	1036:1124	This applies to both a commercial chitosan and chitosans soluble at physiological pH. Incorporation of these chitosans in the raft monolayers was confirmed in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments, where both the tail groups and headgroups were found to interact with chitosan.
32408264	5	7	theme	physiological	945:957	arg1	Incorporation					963:975	physiological pH. Incorporation	945:975	physiological pH. Incorporation of these chitosans in the raft monolayers	945:1017	This applies to both a commercial chitosan and chitosans soluble at physiological pH. Incorporation of these chitosans in the raft monolayers was confirmed in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments, where both the tail groups and headgroups were found to interact with chitosan.
32408264	3	8	theme	Langmuir	496:503	arg1	monolayers					505:514	Langmuir monolayers	496:514	Langmuir monolayers	496:514	In this study, we demonstrate that effects from chitosans on Langmuir monolayers are considerably higher if lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol) are used.
32408264	3	9	from	effects	470:476	arg1	monolayers					505:514	Langmuir monolayers	496:514	Langmuir monolayers	496:514	In this study, we demonstrate that effects from chitosans on Langmuir monolayers are considerably higher if lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol) are used.
32408264	3	10	used	used	669:672	arg2	compositions					554:565	lipid raft compositions	543:565	lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol)	543:663	In this study, we demonstrate that effects from chitosans on Langmuir monolayers are considerably higher if lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol) are used.
32408264	4	11	theme	lipid	782:786	arg1	rafts					788:792	lipid rafts	782:792	lipid rafts	782:792	Significantly, measurable effects on the surface pressure isotherms start at 10-6 mg mL-1 for chitosans in lipid rafts, to be compared with 10-2 mg mL-1 for neat dipalmitoyl phosphatidylcholine (DPPC).
32408264	4	12	theme	pressure	724:731	arg1	isotherms					733:741	the surface pressure isotherms	712:741	the surface pressure isotherms	712:741	Significantly, measurable effects on the surface pressure isotherms start at 10-6 mg mL-1 for chitosans in lipid rafts, to be compared with 10-2 mg mL-1 for neat dipalmitoyl phosphatidylcholine (DPPC).
32408264	1	13	theme	lipid	149:153	arg1	composition					155:165	lipid composition	149:165	lipid composition	149:165	Langmuir monolayers have been used as cell membrane models, where lipid composition is normally varied to mimic distinct types of membranes.
32408264	4	14	theme	10-6	752:755	arg1	mg					757:758	mg	757:758	mg	757:758	Significantly, measurable effects on the surface pressure isotherms start at 10-6 mg mL-1 for chitosans in lipid rafts, to be compared with 10-2 mg mL-1 for neat dipalmitoyl phosphatidylcholine (DPPC).
32408264	4	15	from	effects	701:707	arg1	isotherms					733:741	the surface pressure isotherms	712:741	the surface pressure isotherms	712:741	Significantly, measurable effects on the surface pressure isotherms start at 10-6 mg mL-1 for chitosans in lipid rafts, to be compared with 10-2 mg mL-1 for neat dipalmitoyl phosphatidylcholine (DPPC).
32408264	4	16	theme	surface	716:722	arg1	isotherms					733:741	the surface pressure isotherms	712:741	the surface pressure isotherms	712:741	Significantly, measurable effects on the surface pressure isotherms start at 10-6 mg mL-1 for chitosans in lipid rafts, to be compared with 10-2 mg mL-1 for neat dipalmitoyl phosphatidylcholine (DPPC).
32408264	0	17	theme	chitosan	9:16	arg1	effects					18:24	Enhanced chitosan effects	0:24	Enhanced chitosan effects on cell membrane models	0:48	Enhanced chitosan effects on cell membrane models made with lipid raft monolayers.
32408264	5	18	theme	polarization-modulated	1036:1057	arg1	experiments					1114:1124	polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments	1036:1124	polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments	1036:1124	This applies to both a commercial chitosan and chitosans soluble at physiological pH. Incorporation of these chitosans in the raft monolayers was confirmed in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments, where both the tail groups and headgroups were found to interact with chitosan.
32408264	2	19	theme	zwitterionic	287:298	arg1	phospholipids					300:312	only zwitterionic phospholipids	282:312	only zwitterionic phospholipids	282:312	For eukaryotic membranes, for instance, rather than using only zwitterionic phospholipids there is now a trend to employ mixtures to simulate the lipid rafts known to be relevant for various cellular processes.
32408264	3	20	dep	compositions	554:565	arg1	mixtures					576:583	ternary mixtures	568:583	ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC)	568:626	In this study, we demonstrate that effects from chitosans on Langmuir monolayers are considerably higher if lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol) are used.
32408264	3	20	dep	compositions	554:565	arg1	cholesterol					652:662	cholesterol	652:662	cholesterol	652:662	In this study, we demonstrate that effects from chitosans on Langmuir monolayers are considerably higher if lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol) are used.
32408264	3	20	dep	compositions	554:565	arg1	sphingomyelin					629:641	sphingomyelin	629:641	sphingomyelin (SM)	629:646	In this study, we demonstrate that effects from chitosans on Langmuir monolayers are considerably higher if lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol) are used.
32408264	0	21	theme	Enhanced	0:7	arg1	effects					18:24	Enhanced chitosan effects	0:24	Enhanced chitosan effects on cell membrane models	0:48	Enhanced chitosan effects on cell membrane models made with lipid raft monolayers.
32408264	5	22	theme	chitosans	986:994	arg1	Incorporation					963:975	physiological pH. Incorporation	945:975	physiological pH. Incorporation of these chitosans in the raft monolayers	945:1017	This applies to both a commercial chitosan and chitosans soluble at physiological pH. Incorporation of these chitosans in the raft monolayers was confirmed in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments, where both the tail groups and headgroups were found to interact with chitosan.
32408264	1	23	theme	Langmuir	83:90	arg1	models					135:140	cell membrane models	121:140	cell membrane models	121:140	Langmuir monolayers have been used as cell membrane models, where lipid composition is normally varied to mimic distinct types of membranes.
32408264	1	23	theme	Langmuir	83:90	arg1	monolayers					92:101	Langmuir monolayers	83:101	Langmuir monolayers	83:101	Langmuir monolayers have been used as cell membrane models, where lipid composition is normally varied to mimic distinct types of membranes.
32408264	6	24	theme	small	1268:1272	arg1	concentrations					1274:1287	such small concentrations	1263:1287	such small concentrations for chitosans and probably other molecules	1263:1330	Since the effects on membrane models may be observed at such small concentrations for chitosans and probably other molecules, some studies may have to be revisited where neat phospholipids should be replaced by lipid raft compositions.
32408264	3	25	theme	ternary	568:574	arg1	mixtures					576:583	ternary mixtures	568:583	ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC)	568:626	In this study, we demonstrate that effects from chitosans on Langmuir monolayers are considerably higher if lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol) are used.
32408264	3	25	theme	ternary	568:574	arg1	cholesterol					652:662	cholesterol	652:662	cholesterol	652:662	In this study, we demonstrate that effects from chitosans on Langmuir monolayers are considerably higher if lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol) are used.
32408264	3	25	theme	ternary	568:574	arg1	sphingomyelin					629:641	sphingomyelin	629:641	sphingomyelin (SM)	629:646	In this study, we demonstrate that effects from chitosans on Langmuir monolayers are considerably higher if lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol) are used.
32408264	4	26	theme	dipalmitoyl	837:847	arg1	DPPC					870:873	DPPC	870:873	DPPC	870:873	Significantly, measurable effects on the surface pressure isotherms start at 10-6 mg mL-1 for chitosans in lipid rafts, to be compared with 10-2 mg mL-1 for neat dipalmitoyl phosphatidylcholine (DPPC).
32408264	4	26	theme	dipalmitoyl	837:847	arg1	phosphatidylcholine					849:867	neat dipalmitoyl phosphatidylcholine	832:867	neat dipalmitoyl phosphatidylcholine (DPPC)	832:874	Significantly, measurable effects on the surface pressure isotherms start at 10-6 mg mL-1 for chitosans in lipid rafts, to be compared with 10-2 mg mL-1 for neat dipalmitoyl phosphatidylcholine (DPPC).
32408264	5	27	theme	pH.	959:961	arg1	Incorporation					963:975	physiological pH. Incorporation	945:975	physiological pH. Incorporation of these chitosans in the raft monolayers	945:1017	This applies to both a commercial chitosan and chitosans soluble at physiological pH. Incorporation of these chitosans in the raft monolayers was confirmed in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments, where both the tail groups and headgroups were found to interact with chitosan.
32408264	1	28	used	used	113:116	arg2	models					135:140	cell membrane models	121:140	cell membrane models	121:140	Langmuir monolayers have been used as cell membrane models, where lipid composition is normally varied to mimic distinct types of membranes.
32408264	1	28	used	used	113:116	arg2	monolayers					92:101	Langmuir monolayers	83:101	Langmuir monolayers	83:101	Langmuir monolayers have been used as cell membrane models, where lipid composition is normally varied to mimic distinct types of membranes.
32408264	6	29	theme	neat	1377:1380	arg1	phospholipids					1382:1394	neat phospholipids	1377:1394	neat phospholipids	1377:1394	Since the effects on membrane models may be observed at such small concentrations for chitosans and probably other molecules, some studies may have to be revisited where neat phospholipids should be replaced by lipid raft compositions.
32408264	5	30	theme	reflection	1068:1077	arg1	PM-IRRAS					1104:1111	PM-IRRAS	1104:1111	PM-IRRAS	1104:1111	This applies to both a commercial chitosan and chitosans soluble at physiological pH. Incorporation of these chitosans in the raft monolayers was confirmed in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments, where both the tail groups and headgroups were found to interact with chitosan.
32408264	5	30	theme	reflection	1068:1077	arg1	spectroscopy					1090:1101	infrared reflection absorption spectroscopy	1059:1101	polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments	1036:1124	This applies to both a commercial chitosan and chitosans soluble at physiological pH. Incorporation of these chitosans in the raft monolayers was confirmed in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments, where both the tail groups and headgroups were found to interact with chitosan.
32408264	4	31	theme	neat	832:835	arg1	DPPC					870:873	DPPC	870:873	DPPC	870:873	Significantly, measurable effects on the surface pressure isotherms start at 10-6 mg mL-1 for chitosans in lipid rafts, to be compared with 10-2 mg mL-1 for neat dipalmitoyl phosphatidylcholine (DPPC).
32408264	4	31	theme	neat	832:835	arg1	phosphatidylcholine					849:867	neat dipalmitoyl phosphatidylcholine	832:867	neat dipalmitoyl phosphatidylcholine (DPPC)	832:874	Significantly, measurable effects on the surface pressure isotherms start at 10-6 mg mL-1 for chitosans in lipid rafts, to be compared with 10-2 mg mL-1 for neat dipalmitoyl phosphatidylcholine (DPPC).
32408264	4	32	theme	measurable	690:699	arg1	effects					701:707	measurable effects	690:707	measurable effects on the surface pressure isotherms	690:741	Significantly, measurable effects on the surface pressure isotherms start at 10-6 mg mL-1 for chitosans in lipid rafts, to be compared with 10-2 mg mL-1 for neat dipalmitoyl phosphatidylcholine (DPPC).
32408264	0	33	theme	membrane	34:41	arg1	models					43:48	cell membrane models	29:48	cell membrane models	29:48	Enhanced chitosan effects on cell membrane models made with lipid raft monolayers.
32408264	6	34	theme	such	1263:1266	arg1	concentrations					1274:1287	such small concentrations	1263:1287	such small concentrations for chitosans and probably other molecules	1263:1330	Since the effects on membrane models may be observed at such small concentrations for chitosans and probably other molecules, some studies may have to be revisited where neat phospholipids should be replaced by lipid raft compositions.
32408264	5	35	theme	soluble	934:940	arg1	chitosans					924:932	chitosans	924:932	chitosans soluble at physiological pH. Incorporation of these chitosans in the raft monolayers was confirmed in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments, where both the tail groups and headgroups were found to interact with chitosan	924:1204	This applies to both a commercial chitosan and chitosans soluble at physiological pH. Incorporation of these chitosans in the raft monolayers was confirmed in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments, where both the tail groups and headgroups were found to interact with chitosan.
32408264	0	36	theme	cell	29:32	arg1	models					43:48	cell membrane models	29:48	cell membrane models	29:48	Enhanced chitosan effects on cell membrane models made with lipid raft monolayers.
32408264	3	37	theme	phosphatidyl	600:611	arg1	DPPC					622:625	DPPC	622:625	DPPC	622:625	In this study, we demonstrate that effects from chitosans on Langmuir monolayers are considerably higher if lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol) are used.
32408264	3	37	theme	phosphatidyl	600:611	arg1	choline					613:619	dipalmitoyl phosphatidyl choline	588:619	dipalmitoyl phosphatidyl choline (DPPC)	588:626	In this study, we demonstrate that effects from chitosans on Langmuir monolayers are considerably higher if lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol) are used.
32408264	4	38	theme	mg	757:758	arg1	mL-1					760:763	10-6 mg mL-1	752:763	10-6 mg mL-1 for chitosans in lipid rafts	752:792	Significantly, measurable effects on the surface pressure isotherms start at 10-6 mg mL-1 for chitosans in lipid rafts, to be compared with 10-2 mg mL-1 for neat dipalmitoyl phosphatidylcholine (DPPC).
32408264	4	39	from	chitosans	769:777	arg1	rafts					788:792	lipid rafts	782:792	lipid rafts	782:792	Significantly, measurable effects on the surface pressure isotherms start at 10-6 mg mL-1 for chitosans in lipid rafts, to be compared with 10-2 mg mL-1 for neat dipalmitoyl phosphatidylcholine (DPPC).
32408264	1	40	theme	distinct	195:202	arg1	types					204:208	distinct types	195:208	distinct types of membranes	195:221	Langmuir monolayers have been used as cell membrane models, where lipid composition is normally varied to mimic distinct types of membranes.
32408264	4	41	theme	mg	820:821	arg1	mL-1					823:826	10-2 mg mL-1	815:826	10-2 mg mL-1 for neat dipalmitoyl phosphatidylcholine (DPPC)	815:874	Significantly, measurable effects on the surface pressure isotherms start at 10-6 mg mL-1 for chitosans in lipid rafts, to be compared with 10-2 mg mL-1 for neat dipalmitoyl phosphatidylcholine (DPPC).
32408264	3	42	theme	choline	613:619	arg1	mixtures					576:583	ternary mixtures	568:583	ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC)	568:626	In this study, we demonstrate that effects from chitosans on Langmuir monolayers are considerably higher if lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol) are used.
32408264	3	42	theme	choline	613:619	arg1	cholesterol					652:662	cholesterol	652:662	cholesterol	652:662	In this study, we demonstrate that effects from chitosans on Langmuir monolayers are considerably higher if lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol) are used.
32408264	3	42	theme	choline	613:619	arg1	sphingomyelin					629:641	sphingomyelin	629:641	sphingomyelin (SM)	629:646	In this study, we demonstrate that effects from chitosans on Langmuir monolayers are considerably higher if lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol) are used.
32408264	3	43	theme	lipid	543:547	arg1	compositions					554:565	lipid raft compositions	543:565	lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol)	543:663	In this study, we demonstrate that effects from chitosans on Langmuir monolayers are considerably higher if lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol) are used.
32408264	2	44	theme	cellular	415:422	arg1	processes					424:432	various cellular processes	407:432	various cellular processes	407:432	For eukaryotic membranes, for instance, rather than using only zwitterionic phospholipids there is now a trend to employ mixtures to simulate the lipid rafts known to be relevant for various cellular processes.
32408264	4	45	theme	10-2	815:818	arg1	mL-1					823:826	10-2 mg mL-1	815:826	10-2 mg mL-1 for neat dipalmitoyl phosphatidylcholine (DPPC)	815:874	Significantly, measurable effects on the surface pressure isotherms start at 10-6 mg mL-1 for chitosans in lipid rafts, to be compared with 10-2 mg mL-1 for neat dipalmitoyl phosphatidylcholine (DPPC).
32408264	3	46	theme	raft	549:552	arg1	compositions					554:565	lipid raft compositions	543:565	lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol)	543:663	In this study, we demonstrate that effects from chitosans on Langmuir monolayers are considerably higher if lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol) are used.
32408264	5	47	theme	raft	1003:1006	arg1	monolayers					1008:1017	the raft monolayers	999:1017	the raft monolayers	999:1017	This applies to both a commercial chitosan and chitosans soluble at physiological pH. Incorporation of these chitosans in the raft monolayers was confirmed in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments, where both the tail groups and headgroups were found to interact with chitosan.
32408264	5	48	theme	commercial	900:909	arg1	chitosan					911:918	a commercial chitosan	898:918	a commercial chitosan	898:918	This applies to both a commercial chitosan and chitosans soluble at physiological pH. Incorporation of these chitosans in the raft monolayers was confirmed in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments, where both the tail groups and headgroups were found to interact with chitosan.
32408264	6	49	located	observed	1251:1258	arg2	effects					1217:1223	the effects	1213:1223	the effects on membrane models	1213:1242	Since the effects on membrane models may be observed at such small concentrations for chitosans and probably other molecules, some studies may have to be revisited where neat phospholipids should be replaced by lipid raft compositions.
32408264	6	49	located	observed	1251:1258	arg1	concentrations					1274:1287	such small concentrations	1263:1287	such small concentrations for chitosans and probably other molecules	1263:1330	Since the effects on membrane models may be observed at such small concentrations for chitosans and probably other molecules, some studies may have to be revisited where neat phospholipids should be replaced by lipid raft compositions.
32408264	6	50	from	effects	1217:1223	arg1	models					1237:1242	membrane models	1228:1242	membrane models	1228:1242	Since the effects on membrane models may be observed at such small concentrations for chitosans and probably other molecules, some studies may have to be revisited where neat phospholipids should be replaced by lipid raft compositions.
32408264	6	51	theme	other	1316:1320	arg1	molecules					1322:1330	other molecules	1316:1330	other molecules	1316:1330	Since the effects on membrane models may be observed at such small concentrations for chitosans and probably other molecules, some studies may have to be revisited where neat phospholipids should be replaced by lipid raft compositions.
32408264	3	52	theme	dipalmitoyl	588:598	arg1	DPPC					622:625	DPPC	622:625	DPPC	622:625	In this study, we demonstrate that effects from chitosans on Langmuir monolayers are considerably higher if lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol) are used.
32408264	3	52	theme	dipalmitoyl	588:598	arg1	choline					613:619	dipalmitoyl phosphatidyl choline	588:619	dipalmitoyl phosphatidyl choline (DPPC)	588:626	In this study, we demonstrate that effects from chitosans on Langmuir monolayers are considerably higher if lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol) are used.
32408264	1	53	theme	cell	121:124	arg1	models					135:140	cell membrane models	121:140	cell membrane models	121:140	Langmuir monolayers have been used as cell membrane models, where lipid composition is normally varied to mimic distinct types of membranes.
32408264	1	53	theme	cell	121:124	arg1	monolayers					92:101	Langmuir monolayers	83:101	Langmuir monolayers	83:101	Langmuir monolayers have been used as cell membrane models, where lipid composition is normally varied to mimic distinct types of membranes.
32408264	6	54	theme	lipid	1418:1422	arg1	compositions					1429:1440	lipid raft compositions	1418:1440	lipid raft compositions	1418:1440	Since the effects on membrane models may be observed at such small concentrations for chitosans and probably other molecules, some studies may have to be revisited where neat phospholipids should be replaced by lipid raft compositions.
32408264	0	55	theme	lipid	60:64	arg1	monolayers					71:80	lipid raft monolayers	60:80	lipid raft monolayers	60:80	Enhanced chitosan effects on cell membrane models made with lipid raft monolayers.
32408264	2	56	theme	lipid	370:374	arg1	rafts					376:380	the lipid rafts	366:380	the lipid rafts known to be relevant for various cellular processes	366:432	For eukaryotic membranes, for instance, rather than using only zwitterionic phospholipids there is now a trend to employ mixtures to simulate the lipid rafts known to be relevant for various cellular processes.
32408264	2	57	theme	eukaryotic	228:237	arg1	membranes					239:247	eukaryotic membranes	228:247	eukaryotic membranes	228:247	For eukaryotic membranes, for instance, rather than using only zwitterionic phospholipids there is now a trend to employ mixtures to simulate the lipid rafts known to be relevant for various cellular processes.
32408264	1	58	theme	membrane	126:133	arg1	models					135:140	cell membrane models	121:140	cell membrane models	121:140	Langmuir monolayers have been used as cell membrane models, where lipid composition is normally varied to mimic distinct types of membranes.
32408264	1	58	theme	membrane	126:133	arg1	monolayers					92:101	Langmuir monolayers	83:101	Langmuir monolayers	83:101	Langmuir monolayers have been used as cell membrane models, where lipid composition is normally varied to mimic distinct types of membranes.
32408264	6	59	theme	raft	1424:1427	arg1	compositions					1429:1440	lipid raft compositions	1418:1440	lipid raft compositions	1418:1440	Since the effects on membrane models may be observed at such small concentrations for chitosans and probably other molecules, some studies may have to be revisited where neat phospholipids should be replaced by lipid raft compositions.
32408264	1	60	theme	membranes	213:221	arg1	types					204:208	distinct types	195:208	distinct types of membranes	195:221	Langmuir monolayers have been used as cell membrane models, where lipid composition is normally varied to mimic distinct types of membranes.
32408264	5	61	theme	infrared	1059:1066	arg1	PM-IRRAS					1104:1111	PM-IRRAS	1104:1111	PM-IRRAS	1104:1111	This applies to both a commercial chitosan and chitosans soluble at physiological pH. Incorporation of these chitosans in the raft monolayers was confirmed in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments, where both the tail groups and headgroups were found to interact with chitosan.
32408264	5	61	theme	infrared	1059:1066	arg1	spectroscopy					1090:1101	infrared reflection absorption spectroscopy	1059:1101	polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments	1036:1124	This applies to both a commercial chitosan and chitosans soluble at physiological pH. Incorporation of these chitosans in the raft monolayers was confirmed in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments, where both the tail groups and headgroups were found to interact with chitosan.
32408264	2	62	theme	various	407:413	arg1	processes					424:432	various cellular processes	407:432	various cellular processes	407:432	For eukaryotic membranes, for instance, rather than using only zwitterionic phospholipids there is now a trend to employ mixtures to simulate the lipid rafts known to be relevant for various cellular processes.
32408264	3	63	from	chitosans	483:491	arg1	effects					470:476	effects	470:476	effects from chitosans on Langmuir monolayers	470:514	In this study, we demonstrate that effects from chitosans on Langmuir monolayers are considerably higher if lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol) are used.
32771508	1	0	theme	composite	324:332	arg1	materials					334:342	lanthanide rare earth ions-doped composite materials	291:342	lanthanide rare earth ions-doped composite materials	291:342	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	2	1	dep	the	463:465	arg1	help					467:470	help	467:470	help	467:470	The photoluminescent materials were prepared via in-situ chemical deposition of rare earth ions onto CMC surface with the help of carboxyl groups contained on the surface of CMC.
32771508	2	2	theme	photoluminescent	349:364	arg1	materials					366:374	The photoluminescent materials	345:374	The photoluminescent materials	345:374	The photoluminescent materials were prepared via in-situ chemical deposition of rare earth ions onto CMC surface with the help of carboxyl groups contained on the surface of CMC.
32771508	5	3	theme	facile	1204:1209	arg1	process					1211:1217	facile process	1204:1217	facile process without expensive material equipment	1204:1254	The method has mild reaction conditions, facile process without expensive material equipment.
32771508	5	3	theme	facile	1204:1209	arg1	conditions					1192:1201	mild reaction conditions	1178:1201	mild reaction conditions	1178:1201	The method has mild reaction conditions, facile process without expensive material equipment.
32771508	1	4	theme	inorganic	232:240	arg1	chloride					211:218	rare earth chloride	200:218	rare earth chloride	200:218	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	1	4	theme	inorganic	232:240	arg1	material					259:266	inorganic nano luminescent material	232:266	inorganic nano luminescent material	232:266	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	2	5	theme	carboxyl	475:482	arg1	groups					484:489	carboxyl groups	475:489	carboxyl groups contained on the surface of CMC	475:521	The photoluminescent materials were prepared via in-situ chemical deposition of rare earth ions onto CMC surface with the help of carboxyl groups contained on the surface of CMC.
32771508	0	6	from	preparation	13:23	arg1	anti-counterfeiting					96:114	anti-counterfeiting	96:114	anti-counterfeiting	96:114	Controllable preparation of carboxymethyl cellulose/LaF3:Eu3+ composites and its application in anti-counterfeiting.
32771508	4	7	theme	fluorescence	1023:1034	arg1	intensity					1036:1044	the fluorescence intensity	1019:1044	the fluorescence intensity	1019:1044	The results showed that CMC/LaF3:Eu3+ composites were successfully prepared, and the fluorescence intensity can be organically controlled by the reaction temperature, doping ratio and doping concentration of rare earth ions.
32771508	6	8	theme	packaging	1454:1462	arg1	films					1464:1468	large-scale functional packaging films	1431:1468	large-scale functional packaging films	1431:1468	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	1	9	theme	nano	242:245	arg1	chloride					211:218	rare earth chloride	200:218	rare earth chloride	200:218	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	1	9	theme	nano	242:245	arg1	material					259:266	inorganic nano luminescent material	232:266	inorganic nano luminescent material	232:266	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	2	10	theme	CMC	519:521	arg1	surface					508:514	the surface	504:514	the surface of CMC	504:521	The photoluminescent materials were prepared via in-situ chemical deposition of rare earth ions onto CMC surface with the help of carboxyl groups contained on the surface of CMC.
32771508	6	11	theme	functional	1443:1452	arg1	films					1464:1468	large-scale functional packaging films	1431:1468	large-scale functional packaging films	1431:1468	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	1	12	theme	luminescent	247:257	arg1	chloride					211:218	rare earth chloride	200:218	rare earth chloride	200:218	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	1	12	theme	luminescent	247:257	arg1	material					259:266	inorganic nano luminescent material	232:266	inorganic nano luminescent material	232:266	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	5	13	contain	has	1174:1176	arg2	conditions					1192:1201	mild reaction conditions	1178:1201	mild reaction conditions	1178:1201	The method has mild reaction conditions, facile process without expensive material equipment.
32771508	5	13	contain	has	1174:1176	arg1	method					1167:1172	The method	1163:1172	The method	1163:1172	The method has mild reaction conditions, facile process without expensive material equipment.
32771508	5	13	contain	has	1174:1176	arg2	process					1211:1217	facile process	1204:1217	facile process without expensive material equipment	1204:1254	The method has mild reaction conditions, facile process without expensive material equipment.
32771508	4	14	theme	reaction	1083:1090	arg1	temperature					1092:1102	the reaction temperature	1079:1102	the reaction temperature	1079:1102	The results showed that CMC/LaF3:Eu3+ composites were successfully prepared, and the fluorescence intensity can be organically controlled by the reaction temperature, doping ratio and doping concentration of rare earth ions.
32771508	6	15	theme	fluorescence	1335:1346	arg1	intensity					1348:1356	good fluorescence intensity	1330:1356	good fluorescence intensity	1330:1356	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	6	15	theme	fluorescence	1335:1346	arg1	properties					1397:1406	excellent mechanical properties	1376:1406	excellent mechanical properties	1376:1406	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	6	15	theme	fluorescence	1335:1346	arg1	transparency					1359:1370	transparency	1359:1370	transparency	1359:1370	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	2	16	theme	chemical	402:409	arg1	deposition					411:420	in-situ chemical deposition	394:420	in-situ chemical deposition of rare earth ions onto CMC surface with the help of carboxyl groups contained on the surface of CMC	394:521	The photoluminescent materials were prepared via in-situ chemical deposition of rare earth ions onto CMC surface with the help of carboxyl groups contained on the surface of CMC.
32771508	5	17	theme	mild	1178:1181	arg1	process					1211:1217	facile process	1204:1217	facile process without expensive material equipment	1204:1254	The method has mild reaction conditions, facile process without expensive material equipment.
32771508	5	17	theme	mild	1178:1181	arg1	conditions					1192:1201	mild reaction conditions	1178:1201	mild reaction conditions	1178:1201	The method has mild reaction conditions, facile process without expensive material equipment.
32771508	3	18	theme	FT-IR	713:717	arg1	spectra					719:725	FT-IR spectra	713:725	FT-IR spectra (FTIR)	713:732	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	3	18	theme	FT-IR	713:717	arg1	FTIR					728:731	FTIR	728:731	FTIR	728:731	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	6	19	theme	good	1330:1333	arg1	intensity					1348:1356	good fluorescence intensity	1330:1356	good fluorescence intensity	1330:1356	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	6	19	theme	good	1330:1333	arg1	properties					1397:1406	excellent mechanical properties	1376:1406	excellent mechanical properties	1376:1406	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	6	19	theme	good	1330:1333	arg1	transparency					1359:1370	transparency	1359:1370	transparency	1359:1370	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	0	20	theme	Controllable	0:11	arg1	preparation					13:23	Controllable preparation	0:23	Controllable preparation of carboxymethyl cellulose/LaF3:Eu3+ composites	0:71	Controllable preparation of carboxymethyl cellulose/LaF3:Eu3+ composites and its application in anti-counterfeiting.
32771508	3	21	theme	X-ray	860:864	arg1	XRD					879:881	XRD	879:881	XRD	879:881	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	3	21	theme	X-ray	860:864	arg1	diffraction					866:876	X-ray diffraction	860:876	X-ray diffraction (XRD)	860:882	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	2	22	theme	in-situ	394:400	arg1	deposition					411:420	in-situ chemical deposition	394:420	in-situ chemical deposition of rare earth ions onto CMC surface with the help of carboxyl groups contained on the surface of CMC	394:521	The photoluminescent materials were prepared via in-situ chemical deposition of rare earth ions onto CMC surface with the help of carboxyl groups contained on the surface of CMC.
32771508	3	23	theme	CMC	649:651	arg1	elements					592:599	surface elements	584:599	surface elements	584:599	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	3	23	theme	CMC	649:651	arg1	stability					636:644	thermal stability	628:644	thermal stability	628:644	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	3	23	theme	CMC	649:651	arg1	properties					552:561	fluorescence properties	539:561	fluorescence properties	539:561	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	3	23	theme	CMC	649:651	arg1	structure					614:622	crystalline structure	602:622	crystalline structure	602:622	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	3	23	theme	CMC	649:651	arg1	structure					528:536	The structure	524:536	The structure	524:536	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	3	23	theme	CMC	649:651	arg1	morphology					572:581	surface morphology	564:581	surface morphology	564:581	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	6	24	used	used	1422:1425	arg2	properties					1397:1406	excellent mechanical properties	1376:1406	excellent mechanical properties	1376:1406	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	6	24	used	used	1422:1425	arg2	transparency					1359:1370	transparency	1359:1370	transparency	1359:1370	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	6	24	used	used	1422:1425	arg2	intensity					1348:1356	good fluorescence intensity	1330:1356	good fluorescence intensity	1330:1356	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	4	25	theme	ions	1157:1160	arg1	temperature					1092:1102	the reaction temperature	1079:1102	the reaction temperature	1079:1102	The results showed that CMC/LaF3:Eu3+ composites were successfully prepared, and the fluorescence intensity can be organically controlled by the reaction temperature, doping ratio and doping concentration of rare earth ions.
32771508	4	25	theme	ions	1157:1160	arg1	ratio					1112:1116	doping ratio	1105:1116	doping ratio	1105:1116	The results showed that CMC/LaF3:Eu3+ composites were successfully prepared, and the fluorescence intensity can be organically controlled by the reaction temperature, doping ratio and doping concentration of rare earth ions.
32771508	4	25	theme	ions	1157:1160	arg1	concentration					1129:1141	doping concentration	1122:1141	doping concentration	1122:1141	The results showed that CMC/LaF3:Eu3+ composites were successfully prepared, and the fluorescence intensity can be organically controlled by the reaction temperature, doping ratio and doping concentration of rare earth ions.
32771508	1	26	theme	materials	334:342	arg1	series					281:286	a series	279:286	a series of lanthanide rare earth ions-doped composite materials	279:342	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	3	27	theme	photoluminescence	735:751	arg1	PL					762:763	PL	762:763	PL	762:763	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	3	27	theme	photoluminescence	735:751	arg1	spectra					753:759	photoluminescence spectra	735:759	photoluminescence spectra (PL)	735:764	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	3	28	theme	surface	564:570	arg1	morphology					572:581	surface morphology	564:581	surface morphology	564:581	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	3	29	theme	thermal	888:894	arg1	TGA					918:920	TGA	918:920	TGA	918:920	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	3	29	theme	thermal	888:894	arg1	analyses					908:915	thermal gravimetric analyses	888:915	thermal gravimetric analyses (TGA)	888:921	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	1	30	theme	raw	186:188	arg1	cellulose					158:166	sodium carboxymethyl cellulose	137:166	sodium carboxymethyl cellulose (CMC)	137:172	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	1	30	theme	raw	186:188	arg1	material					190:197	raw material	186:197	raw material	186:197	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	0	31	theme	carboxymethyl	28:40	arg1	composites					62:71	carboxymethyl cellulose/LaF3:Eu3+ composites	28:71	carboxymethyl cellulose/LaF3:Eu3+ composites	28:71	Controllable preparation of carboxymethyl cellulose/LaF3:Eu3+ composites and its application in anti-counterfeiting.
32771508	2	32	with	surface	450:456	arg1	the					463:465	the	463:465	the	463:465	The photoluminescent materials were prepared via in-situ chemical deposition of rare earth ions onto CMC surface with the help of carboxyl groups contained on the surface of CMC.
32771508	3	33	theme	fluorescence	539:550	arg1	properties					552:561	fluorescence properties	539:561	fluorescence properties	539:561	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	3	34	theme	gravimetric	896:906	arg1	TGA					918:920	TGA	918:920	TGA	918:920	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	3	34	theme	gravimetric	896:906	arg1	analyses					908:915	thermal gravimetric analyses	888:915	thermal gravimetric analyses (TGA)	888:921	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	2	35	theme	earth	430:434	arg1	ions					436:439	rare earth ions	425:439	rare earth ions	425:439	The photoluminescent materials were prepared via in-situ chemical deposition of rare earth ions onto CMC surface with the help of carboxyl groups contained on the surface of CMC.
32771508	2	36	theme	ions	436:439	arg1	deposition					411:420	in-situ chemical deposition	394:420	in-situ chemical deposition of rare earth ions onto CMC surface with the help of carboxyl groups contained on the surface of CMC	394:521	The photoluminescent materials were prepared via in-situ chemical deposition of rare earth ions onto CMC surface with the help of carboxyl groups contained on the surface of CMC.
32771508	2	37	theme	CMC	446:448	arg1	surface					450:456	CMC surface	446:456	CMC surface with the help of carboxyl groups contained on the surface of CMC	446:521	The photoluminescent materials were prepared via in-situ chemical deposition of rare earth ions onto CMC surface with the help of carboxyl groups contained on the surface of CMC.
32771508	2	38	theme	rare	425:428	arg1	ions					436:439	rare earth ions	425:439	rare earth ions	425:439	The photoluminescent materials were prepared via in-situ chemical deposition of rare earth ions onto CMC surface with the help of carboxyl groups contained on the surface of CMC.
32771508	6	39	theme	anti-counterfeiting	1295:1313	arg1	film					1315:1318	the as-prepared transparent anti-counterfeiting film	1267:1318	the as-prepared transparent anti-counterfeiting film	1267:1318	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	0	40	from	application	81:91	arg1	anti-counterfeiting					96:114	anti-counterfeiting	96:114	anti-counterfeiting	96:114	Controllable preparation of carboxymethyl cellulose/LaF3:Eu3+ composites and its application in anti-counterfeiting.
32771508	3	41	theme	surface	584:590	arg1	elements					592:599	surface elements	584:599	surface elements	584:599	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	6	42	contain	possessed	1320:1328	arg2	properties					1397:1406	excellent mechanical properties	1376:1406	excellent mechanical properties	1376:1406	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	6	42	contain	possessed	1320:1328	arg1	film					1315:1318	the as-prepared transparent anti-counterfeiting film	1267:1318	the as-prepared transparent anti-counterfeiting film	1267:1318	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	6	42	contain	possessed	1320:1328	arg2	transparency					1359:1370	transparency	1359:1370	transparency	1359:1370	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	6	42	contain	possessed	1320:1328	arg2	intensity					1348:1356	good fluorescence intensity	1330:1356	good fluorescence intensity	1330:1356	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	1	43	theme	rare	200:203	arg1	earth					205:209	rare earth	200:209	rare earth chloride	200:218	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	0	44	theme	cellulose/LaF3	42:55	arg1	composites					62:71	carboxymethyl cellulose/LaF3:Eu3+ composites	28:71	carboxymethyl cellulose/LaF3:Eu3+ composites	28:71	Controllable preparation of carboxymethyl cellulose/LaF3:Eu3+ composites and its application in anti-counterfeiting.
32771508	6	45	theme	transparent	1283:1293	arg1	film					1315:1318	the as-prepared transparent anti-counterfeiting film	1267:1318	the as-prepared transparent anti-counterfeiting film	1267:1318	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	3	46	theme	thermal	628:634	arg1	stability					636:644	thermal stability	628:644	thermal stability	628:644	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	5	47	theme	reaction	1183:1190	arg1	process					1211:1217	facile process	1204:1217	facile process without expensive material equipment	1204:1254	The method has mild reaction conditions, facile process without expensive material equipment.
32771508	5	47	theme	reaction	1183:1190	arg1	conditions					1192:1201	mild reaction conditions	1178:1201	mild reaction conditions	1178:1201	The method has mild reaction conditions, facile process without expensive material equipment.
32771508	1	48	theme	earth	205:209	arg1	chloride					211:218	rare earth chloride	200:218	rare earth chloride	200:218	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	1	48	theme	earth	205:209	arg1	material					259:266	inorganic nano luminescent material	232:266	inorganic nano luminescent material	232:266	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	0	49	theme	composites	62:71	arg1	application					81:91	its application	77:91	its application in anti-counterfeiting	77:114	Controllable preparation of carboxymethyl cellulose/LaF3:Eu3+ composites and its application in anti-counterfeiting.
32771508	0	49	theme	composites	62:71	arg1	preparation					13:23	Controllable preparation	0:23	Controllable preparation of carboxymethyl cellulose/LaF3:Eu3+ composites	0:71	Controllable preparation of carboxymethyl cellulose/LaF3:Eu3+ composites and its application in anti-counterfeiting.
32771508	1	50	theme	lanthanide	291:300	arg1	earth					307:311	lanthanide rare earth	291:311	lanthanide rare earth ions-doped composite materials	291:342	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	6	51	theme	as-prepared	1271:1281	arg1	film					1315:1318	the as-prepared transparent anti-counterfeiting film	1267:1318	the as-prepared transparent anti-counterfeiting film	1267:1318	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	6	52	theme	excellent	1376:1384	arg1	intensity					1348:1356	good fluorescence intensity	1330:1356	good fluorescence intensity	1330:1356	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	6	52	theme	excellent	1376:1384	arg1	properties					1397:1406	excellent mechanical properties	1376:1406	excellent mechanical properties	1376:1406	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	6	53	theme	large-scale	1431:1441	arg1	films					1464:1468	large-scale functional packaging films	1431:1468	large-scale functional packaging films	1431:1468	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	0	54	theme	Eu3+	57:60	arg1	composites					62:71	carboxymethyl cellulose/LaF3:Eu3+ composites	28:71	carboxymethyl cellulose/LaF3:Eu3+ composites	28:71	Controllable preparation of carboxymethyl cellulose/LaF3:Eu3+ composites and its application in anti-counterfeiting.
32771508	1	55	theme	rare	302:305	arg1	earth					307:311	lanthanide rare earth	291:311	lanthanide rare earth ions-doped composite materials	291:342	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	5	56	theme	expensive	1227:1235	arg1	equipment					1246:1254	expensive material equipment	1227:1254	expensive material equipment	1227:1254	The method has mild reaction conditions, facile process without expensive material equipment.
32771508	3	57	theme	surface	670:676	arg1	modification					678:689	surface modification	670:689	surface modification	670:689	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	2	58	contain	contained	491:499	arg1	surface					508:514	the surface	504:514	the surface of CMC	504:521	The photoluminescent materials were prepared via in-situ chemical deposition of rare earth ions onto CMC surface with the help of carboxyl groups contained on the surface of CMC.
32771508	2	58	contain	contained	491:499	arg2	groups					484:489	carboxyl groups	475:489	carboxyl groups contained on the surface of CMC	475:521	The photoluminescent materials were prepared via in-situ chemical deposition of rare earth ions onto CMC surface with the help of carboxyl groups contained on the surface of CMC.
32771508	3	59	theme	photoelectron	826:838	arg1	XPS					854:856	XPS	854:856	XPS	854:856	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	3	59	theme	photoelectron	826:838	arg1	spectroscopy					840:851	X-ray photoelectron spectroscopy	820:851	X-ray photoelectron spectroscopy (XPS)	820:857	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	1	60	theme	earth	307:311	arg1	materials					334:342	lanthanide rare earth ions-doped composite materials	291:342	lanthanide rare earth ions-doped composite materials	291:342	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	1	61	used	used	224:227	arg2	material					259:266	inorganic nano luminescent material	232:266	inorganic nano luminescent material	232:266	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	1	61	used	used	224:227	arg2	chloride					211:218	rare earth chloride	200:218	rare earth chloride	200:218	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	4	62	theme	rare	1146:1149	arg1	earth					1151:1155	rare earth	1146:1155	rare earth ions	1146:1160	The results showed that CMC/LaF3:Eu3+ composites were successfully prepared, and the fluorescence intensity can be organically controlled by the reaction temperature, doping ratio and doping concentration of rare earth ions.
32771508	1	63	theme	sodium	137:142	arg1	CMC					169:171	CMC	169:171	CMC	169:171	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	1	63	theme	sodium	137:142	arg1	material					190:197	raw material	186:197	raw material	186:197	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	1	63	theme	sodium	137:142	arg1	cellulose					158:166	sodium carboxymethyl cellulose	137:166	sodium carboxymethyl cellulose (CMC)	137:172	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	4	64	theme	earth	1151:1155	arg1	ions					1157:1160	rare earth ions	1146:1160	rare earth ions	1146:1160	The results showed that CMC/LaF3:Eu3+ composites were successfully prepared, and the fluorescence intensity can be organically controlled by the reaction temperature, doping ratio and doping concentration of rare earth ions.
32771508	3	65	theme	scanning	782:789	arg1	FESEM					812:816	FESEM	812:816	FESEM	812:816	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	3	65	theme	scanning	782:789	arg1	microscope					800:809	field emission scanning electron microscope	767:809	field emission scanning electron microscope (FESEM)	767:817	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	5	66	theme	material	1237:1244	arg1	equipment					1246:1254	expensive material equipment	1227:1254	expensive material equipment	1227:1254	The method has mild reaction conditions, facile process without expensive material equipment.
32771508	1	67	theme	carboxymethyl	144:156	arg1	CMC					169:171	CMC	169:171	CMC	169:171	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	1	67	theme	carboxymethyl	144:156	arg1	material					190:197	raw material	186:197	raw material	186:197	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	1	67	theme	carboxymethyl	144:156	arg1	cellulose					158:166	sodium carboxymethyl cellulose	137:166	sodium carboxymethyl cellulose (CMC)	137:172	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	3	68	theme	electron	791:798	arg1	FESEM					812:816	FESEM	812:816	FESEM	812:816	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	3	68	theme	electron	791:798	arg1	microscope					800:809	field emission scanning electron microscope	767:809	field emission scanning electron microscope (FESEM)	767:817	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	6	69	theme	mechanical	1386:1395	arg1	intensity					1348:1356	good fluorescence intensity	1330:1356	good fluorescence intensity	1330:1356	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	6	69	theme	mechanical	1386:1395	arg1	properties					1397:1406	excellent mechanical properties	1376:1406	excellent mechanical properties	1376:1406	Moreover, the as-prepared transparent anti-counterfeiting film possessed good fluorescence intensity, transparency and excellent mechanical properties, which can be used for large-scale functional packaging films.
32771508	1	70	used	used	178:181	arg2	CMC					169:171	CMC	169:171	CMC	169:171	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	1	70	used	used	178:181	arg2	cellulose					158:166	sodium carboxymethyl cellulose	137:166	sodium carboxymethyl cellulose (CMC)	137:172	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	1	70	used	used	178:181	arg2	material					190:197	raw material	186:197	raw material	186:197	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	4	71	theme	doping	1105:1110	arg1	ratio					1112:1116	doping ratio	1105:1116	doping ratio	1105:1116	The results showed that CMC/LaF3:Eu3+ composites were successfully prepared, and the fluorescence intensity can be organically controlled by the reaction temperature, doping ratio and doping concentration of rare earth ions.
32771508	3	72	theme	X-ray	820:824	arg1	XPS					854:856	XPS	854:856	XPS	854:856	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	3	72	theme	X-ray	820:824	arg1	spectroscopy					840:851	X-ray photoelectron spectroscopy	820:851	X-ray photoelectron spectroscopy (XPS)	820:857	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	3	73	theme	field	767:771	arg1	FESEM					812:816	FESEM	812:816	FESEM	812:816	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	3	73	theme	field	767:771	arg1	microscope					800:809	field emission scanning electron microscope	767:809	field emission scanning electron microscope (FESEM)	767:817	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	4	74	theme	CMC/LaF3	962:969	arg1	composites					976:985	CMC/LaF3:Eu3+ composites	962:985	CMC/LaF3:Eu3+ composites	962:985	The results showed that CMC/LaF3:Eu3+ composites were successfully prepared, and the fluorescence intensity can be organically controlled by the reaction temperature, doping ratio and doping concentration of rare earth ions.
32771508	1	75	theme	ions-doped	313:322	arg1	materials					334:342	lanthanide rare earth ions-doped composite materials	291:342	lanthanide rare earth ions-doped composite materials	291:342	In this experiment, sodium carboxymethyl cellulose (CMC) was used as raw material, rare earth chloride was used as inorganic nano luminescent material to prepare a series of lanthanide rare earth ions-doped composite materials.
32771508	3	76	theme	crystalline	602:612	arg1	structure					614:622	crystalline structure	602:622	crystalline structure	602:622	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	4	77	theme	doping	1122:1127	arg1	concentration					1129:1141	doping concentration	1122:1141	doping concentration	1122:1141	The results showed that CMC/LaF3:Eu3+ composites were successfully prepared, and the fluorescence intensity can be organically controlled by the reaction temperature, doping ratio and doping concentration of rare earth ions.
32771508	4	78	theme	Eu3+	971:974	arg1	composites					976:985	CMC/LaF3:Eu3+ composites	962:985	CMC/LaF3:Eu3+ composites	962:985	The results showed that CMC/LaF3:Eu3+ composites were successfully prepared, and the fluorescence intensity can be organically controlled by the reaction temperature, doping ratio and doping concentration of rare earth ions.
32771508	3	79	theme	emission	773:780	arg1	FESEM					812:816	FESEM	812:816	FESEM	812:816	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32771508	3	79	theme	emission	773:780	arg1	microscope					800:809	field emission scanning electron microscope	767:809	field emission scanning electron microscope (FESEM)	767:817	The structure, fluorescence properties, surface morphology, surface elements, crystalline structure and thermal stability of CMC before and after surface modification were characterized by FT-IR spectra (FTIR), photoluminescence spectra (PL), field emission scanning electron microscope (FESEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA), respectively.
32350094	5	0	theme	higher-order	755:766	arg1	structure					768:776	higher-order structure	755:776	higher-order structure of the capsule	755:791	The capsular polysaccharides of C. neoformans strains differ in repeating motifs and therefore vary in the number of hydroxyl groups, which, along with higher-order structure of the capsule, may contribute to the variation in hydrophobicity that we observed.
32350094	10	1	theme	host-pathogen	1860:1872	arg1	interaction					1874:1884	host-pathogen interaction	1860:1884	host-pathogen interaction	1860:1884	Studying variation in biophysical properties like CSH gives us insight into the dynamic host-predator interaction and host-pathogen interaction in a damage-response framework.
32350094	7	2	theme	protective	1105:1114	arg1	18B7					1126:1129	18B7	1126:1129	18B7	1126:1129	For C. neoformans, CSH correlated with phagocytosis by natural soil predator Acanthamoeba castellanii Furthermore, capsular binding of the protective antibody (18B7), but not the nonprotective antibody (13F1), altered the CSH of C. neoformans strains.
32350094	7	2	theme	protective	1105:1114	arg1	antibody					1116:1123	the protective antibody	1101:1123	the protective antibody (18B7)	1101:1130	For C. neoformans, CSH correlated with phagocytosis by natural soil predator Acanthamoeba castellanii Furthermore, capsular binding of the protective antibody (18B7), but not the nonprotective antibody (13F1), altered the CSH of C. neoformans strains.
32350094	9	3	theme	surface	1524:1530	arg1	CSH					1548:1550	CSH	1548:1550	CSH	1548:1550	The affinity of the cell surface for water, defined by the cell surface hydrophobicity (CSH), is a biophysical parameter that varies among different strains of Cryptococcus neoformans The CSH influences the phagocytosis of the yeast by its natural predator in the soil, the amoeba.
32350094	9	3	theme	surface	1524:1530	arg1	hydrophobicity					1532:1545	the cell surface hydrophobicity	1515:1545	the cell surface hydrophobicity (CSH)	1515:1551	The affinity of the cell surface for water, defined by the cell surface hydrophobicity (CSH), is a biophysical parameter that varies among different strains of Cryptococcus neoformans The CSH influences the phagocytosis of the yeast by its natural predator in the soil, the amoeba.
32350094	4	4	theme	large	529:533	arg1	differences					535:545	large differences	529:545	large differences in the CSH of some C. neoformans and C. gattii strains	529:600	In this study, we report large differences in the CSH of some C. neoformans and C. gattii strains.
32350094	7	5	theme	antibody	1116:1123	arg1	binding					1090:1096	capsular binding	1081:1096	capsular binding of the protective antibody (18B7), but not the nonprotective antibody (13F1),	1081:1174	For C. neoformans, CSH correlated with phagocytosis by natural soil predator Acanthamoeba castellanii Furthermore, capsular binding of the protective antibody (18B7), but not the nonprotective antibody (13F1), altered the CSH of C. neoformans strains.
32350094	7	6	theme	predator	1034:1041	arg1	castellanii					1056:1066	natural soil predator Acanthamoeba castellanii	1021:1066	natural soil predator Acanthamoeba castellanii	1021:1066	For C. neoformans, CSH correlated with phagocytosis by natural soil predator Acanthamoeba castellanii Furthermore, capsular binding of the protective antibody (18B7), but not the nonprotective antibody (13F1), altered the CSH of C. neoformans strains.
32350094	8	7	with	interaction	1352:1362	arg1	environment					1393:1403	its environment	1389:1403	its environment	1389:1403	Variability in CSH could be an important characteristic in comparing the biological properties of cryptococcal strains.IMPORTANCE The interaction of a microbial cell with its environment is influenced by the biophysical properties of a cell.
32350094	5	8	theme	groups	729:734	arg1	number					710:715	the number	706:715	the number of hydroxyl groups	706:734	The capsular polysaccharides of C. neoformans strains differ in repeating motifs and therefore vary in the number of hydroxyl groups, which, along with higher-order structure of the capsule, may contribute to the variation in hydrophobicity that we observed.
32350094	0	9	from	Variation	0:8	arg1	Hydrophobicity					26:39	Cell Surface Hydrophobicity	13:39	Cell Surface Hydrophobicity	13:39	Variation in Cell Surface Hydrophobicity among Cryptococcus neoformans Strains Influences Interactions with Amoebas.
32350094	5	10	theme	repeating	667:675	arg1	motifs					677:682	repeating motifs	667:682	repeating motifs	667:682	The capsular polysaccharides of C. neoformans strains differ in repeating motifs and therefore vary in the number of hydroxyl groups, which, along with higher-order structure of the capsule, may contribute to the variation in hydrophobicity that we observed.
32350094	0	11	with	Interactions	90:101	arg1	Amoebas					108:114	Amoebas	108:114	Amoebas	108:114	Variation in Cell Surface Hydrophobicity among Cryptococcus neoformans Strains Influences Interactions with Amoebas.
32350094	4	12	theme	strains	594:600	arg1	CSH					554:556	the CSH	550:556	the CSH of some C. neoformans and C. gattii strains	550:600	In this study, we report large differences in the CSH of some C. neoformans and C. gattii strains.
32350094	9	13	theme	cell	1519:1522	arg1	CSH					1548:1550	CSH	1548:1550	CSH	1548:1550	The affinity of the cell surface for water, defined by the cell surface hydrophobicity (CSH), is a biophysical parameter that varies among different strains of Cryptococcus neoformans The CSH influences the phagocytosis of the yeast by its natural predator in the soil, the amoeba.
32350094	9	13	theme	cell	1519:1522	arg1	hydrophobicity					1532:1545	the cell surface hydrophobicity	1515:1545	the cell surface hydrophobicity (CSH)	1515:1551	The affinity of the cell surface for water, defined by the cell surface hydrophobicity (CSH), is a biophysical parameter that varies among different strains of Cryptococcus neoformans The CSH influences the phagocytosis of the yeast by its natural predator in the soil, the amoeba.
32350094	6	14	theme	wall	881:884	arg1	composition					886:896	cell wall composition	876:896	cell wall composition	876:896	We found that cell wall composition, in the context of chitin-chitosan content, does not influence CSH.
32350094	5	15	theme	capsular	607:614	arg1	polysaccharides					616:630	The capsular polysaccharides	603:630	The capsular polysaccharides of C. neoformans strains	603:655	The capsular polysaccharides of C. neoformans strains differ in repeating motifs and therefore vary in the number of hydroxyl groups, which, along with higher-order structure of the capsule, may contribute to the variation in hydrophobicity that we observed.
32350094	8	16	theme	biological	1291:1300	arg1	properties					1302:1311	the biological properties	1287:1311	the biological properties of cryptococcal strains.IMPORTANCE	1287:1346	Variability in CSH could be an important characteristic in comparing the biological properties of cryptococcal strains.IMPORTANCE The interaction of a microbial cell with its environment is influenced by the biophysical properties of a cell.
32350094	10	17	from	variation	1751:1759	arg1	properties					1776:1785	biophysical properties	1764:1785	biophysical properties like CSH	1764:1794	Studying variation in biophysical properties like CSH gives us insight into the dynamic host-predator interaction and host-pathogen interaction in a damage-response framework.
32350094	4	18	theme	gattii	587:592	arg1	strains					594:600	some C. neoformans and C. gattii strains	561:600	some C. neoformans and C. gattii strains	561:600	In this study, we report large differences in the CSH of some C. neoformans and C. gattii strains.
32350094	7	19	theme	antibody	1159:1166	arg1	binding					1090:1096	capsular binding	1081:1096	capsular binding of the protective antibody (18B7), but not the nonprotective antibody (13F1),	1081:1174	For C. neoformans, CSH correlated with phagocytosis by natural soil predator Acanthamoeba castellanii Furthermore, capsular binding of the protective antibody (18B7), but not the nonprotective antibody (13F1), altered the CSH of C. neoformans strains.
32350094	6	20	theme	cell	876:879	arg1	composition					886:896	cell wall composition	876:896	cell wall composition	876:896	We found that cell wall composition, in the context of chitin-chitosan content, does not influence CSH.
32350094	7	21	theme	capsular	1081:1088	arg1	binding					1090:1096	capsular binding	1081:1096	capsular binding of the protective antibody (18B7), but not the nonprotective antibody (13F1),	1081:1174	For C. neoformans, CSH correlated with phagocytosis by natural soil predator Acanthamoeba castellanii Furthermore, capsular binding of the protective antibody (18B7), but not the nonprotective antibody (13F1), altered the CSH of C. neoformans strains.
32350094	8	22	theme	cryptococcal	1316:1327	arg1	strains.IMPORTANCE					1329:1346	cryptococcal strains.IMPORTANCE	1316:1346	cryptococcal strains.IMPORTANCE	1316:1346	Variability in CSH could be an important characteristic in comparing the biological properties of cryptococcal strains.IMPORTANCE The interaction of a microbial cell with its environment is influenced by the biophysical properties of a cell.
32350094	1	23	theme	significant	197:207	arg1	morbidity					209:217	morbidity	209:217	morbidity	209:217	Cryptococcus neoformans and Cryptococcus gattii are pathogenic fungi that cause significant morbidity and mortality.
32350094	4	24	from	differences	535:545	arg1	CSH					554:556	the CSH	550:556	the CSH of some C. neoformans and C. gattii strains	550:600	In this study, we report large differences in the CSH of some C. neoformans and C. gattii strains.
32350094	9	25	theme	Cryptococcus	1620:1631	arg1	neoformans					1633:1642	Cryptococcus neoformans	1620:1642	Cryptococcus neoformans	1620:1642	The affinity of the cell surface for water, defined by the cell surface hydrophobicity (CSH), is a biophysical parameter that varies among different strains of Cryptococcus neoformans The CSH influences the phagocytosis of the yeast by its natural predator in the soil, the amoeba.
32350094	7	26	theme	nonprotective	1145:1157	arg1	13F1					1169:1172	13F1	1169:1172	13F1	1169:1172	For C. neoformans, CSH correlated with phagocytosis by natural soil predator Acanthamoeba castellanii Furthermore, capsular binding of the protective antibody (18B7), but not the nonprotective antibody (13F1), altered the CSH of C. neoformans strains.
32350094	7	26	theme	nonprotective	1145:1157	arg1	antibody					1159:1166	the nonprotective antibody	1141:1166	the nonprotective antibody (13F1)	1141:1173	For C. neoformans, CSH correlated with phagocytosis by natural soil predator Acanthamoeba castellanii Furthermore, capsular binding of the protective antibody (18B7), but not the nonprotective antibody (13F1), altered the CSH of C. neoformans strains.
32350094	10	27	theme	host-predator	1830:1842	arg1	interaction					1844:1854	the dynamic host-predator interaction	1818:1854	the dynamic host-predator interaction	1818:1854	Studying variation in biophysical properties like CSH gives us insight into the dynamic host-predator interaction and host-pathogen interaction in a damage-response framework.
32350094	10	28	theme	dynamic	1822:1828	arg1	interaction					1844:1854	the dynamic host-predator interaction	1818:1854	the dynamic host-predator interaction	1818:1854	Studying variation in biophysical properties like CSH gives us insight into the dynamic host-predator interaction and host-pathogen interaction in a damage-response framework.
32350094	5	29	theme	neoformans	638:647	arg1	strains					649:655	C. neoformans strains	635:655	C. neoformans strains	635:655	The capsular polysaccharides of C. neoformans strains differ in repeating motifs and therefore vary in the number of hydroxyl groups, which, along with higher-order structure of the capsule, may contribute to the variation in hydrophobicity that we observed.
32350094	2	30	theme	spores	343:348	arg1	adhesion					315:322	the adhesion	311:322	the adhesion of fungal cells or spores to biotic and abiotic surfaces	311:379	Cell surface hydrophobicity (CSH) is a biophysical parameter that influences the adhesion of fungal cells or spores to biotic and abiotic surfaces.
32350094	9	31	theme	neoformans	1633:1642	arg1	strains					1609:1615	different strains	1599:1615	different strains of Cryptococcus neoformans	1599:1642	The affinity of the cell surface for water, defined by the cell surface hydrophobicity (CSH), is a biophysical parameter that varies among different strains of Cryptococcus neoformans The CSH influences the phagocytosis of the yeast by its natural predator in the soil, the amoeba.
32350094	10	32	theme	damage-response	1891:1905	arg1	framework					1907:1915	a damage-response framework	1889:1915	a damage-response framework	1889:1915	Studying variation in biophysical properties like CSH gives us insight into the dynamic host-predator interaction and host-pathogen interaction in a damage-response framework.
32350094	3	33	theme	polysaccharide	410:423	arg1	capsule					425:431	polysaccharide capsule	410:431	polysaccharide capsule that is highly hydrophilic and is a critical determinant of virulence	410:501	C. neoformans is encased by polysaccharide capsule that is highly hydrophilic and is a critical determinant of virulence.
32350094	5	34	theme	strains	649:655	arg1	polysaccharides					616:630	The capsular polysaccharides	603:630	The capsular polysaccharides of C. neoformans strains	603:655	The capsular polysaccharides of C. neoformans strains differ in repeating motifs and therefore vary in the number of hydroxyl groups, which, along with higher-order structure of the capsule, may contribute to the variation in hydrophobicity that we observed.
32350094	9	35	theme	natural	1700:1706	arg1	predator					1708:1715	its natural predator	1696:1715	its natural predator in the soil	1696:1727	The affinity of the cell surface for water, defined by the cell surface hydrophobicity (CSH), is a biophysical parameter that varies among different strains of Cryptococcus neoformans The CSH influences the phagocytosis of the yeast by its natural predator in the soil, the amoeba.
32350094	8	36	theme	strains.IMPORTANCE	1329:1346	arg1	properties					1302:1311	the biological properties	1287:1311	the biological properties of cryptococcal strains.IMPORTANCE	1287:1346	Variability in CSH could be an important characteristic in comparing the biological properties of cryptococcal strains.IMPORTANCE The interaction of a microbial cell with its environment is influenced by the biophysical properties of a cell.
32350094	10	37	theme	us	1802:1803	arg1	insight					1805:1811	us insight	1802:1811	us insight into the dynamic host-predator interaction and host-pathogen interaction	1802:1884	Studying variation in biophysical properties like CSH gives us insight into the dynamic host-predator interaction and host-pathogen interaction in a damage-response framework.
32350094	0	38	theme	Surface	18:24	arg1	Hydrophobicity					26:39	Cell Surface Hydrophobicity	13:39	Cell Surface Hydrophobicity	13:39	Variation in Cell Surface Hydrophobicity among Cryptococcus neoformans Strains Influences Interactions with Amoebas.
32350094	10	39	theme	biophysical	1764:1774	arg1	properties					1776:1785	biophysical properties	1764:1785	biophysical properties like CSH	1764:1794	Studying variation in biophysical properties like CSH gives us insight into the dynamic host-predator interaction and host-pathogen interaction in a damage-response framework.
32350094	7	40	theme	Acanthamoeba	1043:1054	arg1	castellanii					1056:1066	natural soil predator Acanthamoeba castellanii	1021:1066	natural soil predator Acanthamoeba castellanii	1021:1066	For C. neoformans, CSH correlated with phagocytosis by natural soil predator Acanthamoeba castellanii Furthermore, capsular binding of the protective antibody (18B7), but not the nonprotective antibody (13F1), altered the CSH of C. neoformans strains.
32350094	2	41	theme	cells	334:338	arg1	adhesion					315:322	the adhesion	311:322	the adhesion of fungal cells or spores to biotic and abiotic surfaces	311:379	Cell surface hydrophobicity (CSH) is a biophysical parameter that influences the adhesion of fungal cells or spores to biotic and abiotic surfaces.
32350094	0	42	theme	Cell	13:16	arg1	Hydrophobicity					26:39	Cell Surface Hydrophobicity	13:39	Cell Surface Hydrophobicity	13:39	Variation in Cell Surface Hydrophobicity among Cryptococcus neoformans Strains Influences Interactions with Amoebas.
32350094	4	43	theme	neoformans	569:578	arg1	strains					594:600	some C. neoformans and C. gattii strains	561:600	some C. neoformans and C. gattii strains	561:600	In this study, we report large differences in the CSH of some C. neoformans and C. gattii strains.
32350094	2	44	theme	fungal	327:332	arg1	cells					334:338	fungal cells	327:338	fungal cells	327:338	Cell surface hydrophobicity (CSH) is a biophysical parameter that influences the adhesion of fungal cells or spores to biotic and abiotic surfaces.
32350094	3	45	theme	virulence	493:501	arg1	determinant					478:488	a critical determinant	467:488	a critical determinant of virulence	467:501	C. neoformans is encased by polysaccharide capsule that is highly hydrophilic and is a critical determinant of virulence.
32350094	9	46	theme	different	1599:1607	arg1	strains					1609:1615	different strains	1599:1615	different strains of Cryptococcus neoformans	1599:1642	The affinity of the cell surface for water, defined by the cell surface hydrophobicity (CSH), is a biophysical parameter that varies among different strains of Cryptococcus neoformans The CSH influences the phagocytosis of the yeast by its natural predator in the soil, the amoeba.
32350094	7	47	theme	soil	1029:1032	arg1	castellanii					1056:1066	natural soil predator Acanthamoeba castellanii	1021:1066	natural soil predator Acanthamoeba castellanii	1021:1066	For C. neoformans, CSH correlated with phagocytosis by natural soil predator Acanthamoeba castellanii Furthermore, capsular binding of the protective antibody (18B7), but not the nonprotective antibody (13F1), altered the CSH of C. neoformans strains.
32350094	7	48	theme	neoformans	1198:1207	arg1	strains					1209:1215	C. neoformans strains	1195:1215	C. neoformans strains	1195:1215	For C. neoformans, CSH correlated with phagocytosis by natural soil predator Acanthamoeba castellanii Furthermore, capsular binding of the protective antibody (18B7), but not the nonprotective antibody (13F1), altered the CSH of C. neoformans strains.
32350094	8	49	theme	microbial	1369:1377	arg1	cell					1379:1382	a microbial cell	1367:1382	a microbial cell	1367:1382	Variability in CSH could be an important characteristic in comparing the biological properties of cryptococcal strains.IMPORTANCE The interaction of a microbial cell with its environment is influenced by the biophysical properties of a cell.
32350094	3	50	theme	critical	469:476	arg1	determinant					478:488	a critical determinant	467:488	a critical determinant of virulence	467:501	C. neoformans is encased by polysaccharide capsule that is highly hydrophilic and is a critical determinant of virulence.
32350094	6	51	theme	content	933:939	arg1	context					906:912	the context	902:912	the context of chitin-chitosan content	902:939	We found that cell wall composition, in the context of chitin-chitosan content, does not influence CSH.
32350094	5	52	theme	hydroxyl	720:727	arg1	groups					729:734	hydroxyl groups	720:734	hydroxyl groups	720:734	The capsular polysaccharides of C. neoformans strains differ in repeating motifs and therefore vary in the number of hydroxyl groups, which, along with higher-order structure of the capsule, may contribute to the variation in hydrophobicity that we observed.
32350094	8	53	dep	comparing	1277:1285	arg1	influenced					1408:1417	influenced	1408:1417	is influenced by the biophysical properties of a cell	1405:1457	Variability in CSH could be an important characteristic in comparing the biological properties of cryptococcal strains.IMPORTANCE The interaction of a microbial cell with its environment is influenced by the biophysical properties of a cell.
32350094	7	54	theme	C.	1195:1196	arg1	strains					1209:1215	C. neoformans strains	1195:1215	C. neoformans strains	1195:1215	For C. neoformans, CSH correlated with phagocytosis by natural soil predator Acanthamoeba castellanii Furthermore, capsular binding of the protective antibody (18B7), but not the nonprotective antibody (13F1), altered the CSH of C. neoformans strains.
32350094	0	55	theme	neoformans	60:69	arg1	Strains					71:77	Cryptococcus neoformans Strains	47:77	Cryptococcus neoformans Strains	47:77	Variation in Cell Surface Hydrophobicity among Cryptococcus neoformans Strains Influences Interactions with Amoebas.
32350094	6	56	theme	chitin-chitosan	917:931	arg1	content					933:939	chitin-chitosan content	917:939	chitin-chitosan content	917:939	We found that cell wall composition, in the context of chitin-chitosan content, does not influence CSH.
32350094	8	57	theme	biophysical	1426:1436	arg1	properties					1438:1447	the biophysical properties	1422:1447	the biophysical properties of a cell	1422:1457	Variability in CSH could be an important characteristic in comparing the biological properties of cryptococcal strains.IMPORTANCE The interaction of a microbial cell with its environment is influenced by the biophysical properties of a cell.
32350094	5	58	dep	we	849:850	arg1	hydrophobicity					829:842	hydrophobicity	829:842	hydrophobicity that we observed	829:859	The capsular polysaccharides of C. neoformans strains differ in repeating motifs and therefore vary in the number of hydroxyl groups, which, along with higher-order structure of the capsule, may contribute to the variation in hydrophobicity that we observed.
32350094	5	58	dep	we	849:850	arg1	observed					852:859	observed	852:859	observed	852:859	The capsular polysaccharides of C. neoformans strains differ in repeating motifs and therefore vary in the number of hydroxyl groups, which, along with higher-order structure of the capsule, may contribute to the variation in hydrophobicity that we observed.
32350094	2	59	theme	abiotic	364:370	arg1	surfaces					372:379	biotic and abiotic surfaces	353:379	biotic and abiotic surfaces	353:379	Cell surface hydrophobicity (CSH) is a biophysical parameter that influences the adhesion of fungal cells or spores to biotic and abiotic surfaces.
32350094	0	60	theme	Cryptococcus	47:58	arg1	neoformans					60:69	Cryptococcus neoformans	47:69	Cryptococcus neoformans Strains	47:77	Variation in Cell Surface Hydrophobicity among Cryptococcus neoformans Strains Influences Interactions with Amoebas.
32350094	7	61	theme	natural	1021:1027	arg1	castellanii					1056:1066	natural soil predator Acanthamoeba castellanii	1021:1066	natural soil predator Acanthamoeba castellanii	1021:1066	For C. neoformans, CSH correlated with phagocytosis by natural soil predator Acanthamoeba castellanii Furthermore, capsular binding of the protective antibody (18B7), but not the nonprotective antibody (13F1), altered the CSH of C. neoformans strains.
32350094	9	62	from	predator	1708:1715	arg1	soil					1724:1727	the soil	1720:1727	the soil	1720:1727	The affinity of the cell surface for water, defined by the cell surface hydrophobicity (CSH), is a biophysical parameter that varies among different strains of Cryptococcus neoformans The CSH influences the phagocytosis of the yeast by its natural predator in the soil, the amoeba.
32350094	8	63	theme	cell	1379:1382	arg1	interaction					1352:1362	The interaction	1348:1362	The interaction of a microbial cell with its environment	1348:1403	Variability in CSH could be an important characteristic in comparing the biological properties of cryptococcal strains.IMPORTANCE The interaction of a microbial cell with its environment is influenced by the biophysical properties of a cell.
32350094	5	64	from	variation	816:824	arg1	hydrophobicity					829:842	hydrophobicity	829:842	hydrophobicity that we observed	829:859	The capsular polysaccharides of C. neoformans strains differ in repeating motifs and therefore vary in the number of hydroxyl groups, which, along with higher-order structure of the capsule, may contribute to the variation in hydrophobicity that we observed.
32350094	1	65	theme	Cryptococcus	145:156	arg1	gattii					158:163	Cryptococcus gattii	145:163	Cryptococcus gattii	145:163	Cryptococcus neoformans and Cryptococcus gattii are pathogenic fungi that cause significant morbidity and mortality.
32350094	1	65	theme	Cryptococcus	145:156	arg1	fungi					180:184	pathogenic fungi	169:184	pathogenic fungi that cause significant morbidity and mortality	169:231	Cryptococcus neoformans and Cryptococcus gattii are pathogenic fungi that cause significant morbidity and mortality.
32350094	1	65	theme	Cryptococcus	145:156	arg1	neoformans					130:139	Cryptococcus neoformans	117:139	Cryptococcus neoformans	117:139	Cryptococcus neoformans and Cryptococcus gattii are pathogenic fungi that cause significant morbidity and mortality.
32350094	9	66	theme	yeast	1687:1691	arg1	phagocytosis					1667:1678	the phagocytosis	1663:1678	the phagocytosis of the yeast	1663:1691	The affinity of the cell surface for water, defined by the cell surface hydrophobicity (CSH), is a biophysical parameter that varies among different strains of Cryptococcus neoformans The CSH influences the phagocytosis of the yeast by its natural predator in the soil, the amoeba.
32350094	2	67	theme	surface	239:245	arg1	parameter					285:293	a biophysical parameter	271:293	a biophysical parameter that influences the adhesion of fungal cells or spores to biotic and abiotic surfaces	271:379	Cell surface hydrophobicity (CSH) is a biophysical parameter that influences the adhesion of fungal cells or spores to biotic and abiotic surfaces.
32350094	2	67	theme	surface	239:245	arg1	CSH					263:265	CSH	263:265	CSH	263:265	Cell surface hydrophobicity (CSH) is a biophysical parameter that influences the adhesion of fungal cells or spores to biotic and abiotic surfaces.
32350094	2	67	theme	surface	239:245	arg1	hydrophobicity					247:260	Cell surface hydrophobicity	234:260	Cell surface hydrophobicity (CSH)	234:266	Cell surface hydrophobicity (CSH) is a biophysical parameter that influences the adhesion of fungal cells or spores to biotic and abiotic surfaces.
32350094	2	68	theme	biotic	353:358	arg1	surfaces					372:379	biotic and abiotic surfaces	353:379	biotic and abiotic surfaces	353:379	Cell surface hydrophobicity (CSH) is a biophysical parameter that influences the adhesion of fungal cells or spores to biotic and abiotic surfaces.
32350094	1	69	theme	Cryptococcus	117:128	arg1	gattii					158:163	Cryptococcus gattii	145:163	Cryptococcus gattii	145:163	Cryptococcus neoformans and Cryptococcus gattii are pathogenic fungi that cause significant morbidity and mortality.
32350094	1	69	theme	Cryptococcus	117:128	arg1	fungi					180:184	pathogenic fungi	169:184	pathogenic fungi that cause significant morbidity and mortality	169:231	Cryptococcus neoformans and Cryptococcus gattii are pathogenic fungi that cause significant morbidity and mortality.
32350094	1	69	theme	Cryptococcus	117:128	arg1	neoformans					130:139	Cryptococcus neoformans	117:139	Cryptococcus neoformans	117:139	Cryptococcus neoformans and Cryptococcus gattii are pathogenic fungi that cause significant morbidity and mortality.
32350094	2	70	theme	Cell	234:237	arg1	parameter					285:293	a biophysical parameter	271:293	a biophysical parameter that influences the adhesion of fungal cells or spores to biotic and abiotic surfaces	271:379	Cell surface hydrophobicity (CSH) is a biophysical parameter that influences the adhesion of fungal cells or spores to biotic and abiotic surfaces.
32350094	2	70	theme	Cell	234:237	arg1	CSH					263:265	CSH	263:265	CSH	263:265	Cell surface hydrophobicity (CSH) is a biophysical parameter that influences the adhesion of fungal cells or spores to biotic and abiotic surfaces.
32350094	2	70	theme	Cell	234:237	arg1	hydrophobicity					247:260	Cell surface hydrophobicity	234:260	Cell surface hydrophobicity (CSH)	234:266	Cell surface hydrophobicity (CSH) is a biophysical parameter that influences the adhesion of fungal cells or spores to biotic and abiotic surfaces.
32350094	9	71	theme	biophysical	1559:1569	arg1	parameter					1571:1579	a biophysical parameter	1557:1579	a biophysical parameter that varies among different strains of Cryptococcus neoformans	1557:1642	The affinity of the cell surface for water, defined by the cell surface hydrophobicity (CSH), is a biophysical parameter that varies among different strains of Cryptococcus neoformans The CSH influences the phagocytosis of the yeast by its natural predator in the soil, the amoeba.
32350094	9	71	theme	biophysical	1559:1569	arg1	affinity					1464:1471	The affinity	1460:1471	The affinity	1460:1471	The affinity of the cell surface for water, defined by the cell surface hydrophobicity (CSH), is a biophysical parameter that varies among different strains of Cryptococcus neoformans The CSH influences the phagocytosis of the yeast by its natural predator in the soil, the amoeba.
32350094	9	72	theme	surface	1485:1491	arg1	parameter					1571:1579	a biophysical parameter	1557:1579	a biophysical parameter that varies among different strains of Cryptococcus neoformans	1557:1642	The affinity of the cell surface for water, defined by the cell surface hydrophobicity (CSH), is a biophysical parameter that varies among different strains of Cryptococcus neoformans The CSH influences the phagocytosis of the yeast by its natural predator in the soil, the amoeba.
32350094	9	72	theme	surface	1485:1491	arg1	affinity					1464:1471	The affinity	1460:1471	The affinity	1460:1471	The affinity of the cell surface for water, defined by the cell surface hydrophobicity (CSH), is a biophysical parameter that varies among different strains of Cryptococcus neoformans The CSH influences the phagocytosis of the yeast by its natural predator in the soil, the amoeba.
32350094	8	73	theme	important	1249:1257	arg1	Variability					1218:1228	Variability	1218:1228	Variability in CSH	1218:1235	Variability in CSH could be an important characteristic in comparing the biological properties of cryptococcal strains.IMPORTANCE The interaction of a microbial cell with its environment is influenced by the biophysical properties of a cell.
32350094	8	73	theme	important	1249:1257	arg1	characteristic					1259:1272	an important characteristic	1246:1272	an important characteristic	1246:1272	Variability in CSH could be an important characteristic in comparing the biological properties of cryptococcal strains.IMPORTANCE The interaction of a microbial cell with its environment is influenced by the biophysical properties of a cell.
32350094	8	74	theme	cell	1454:1457	arg1	properties					1438:1447	the biophysical properties	1422:1447	the biophysical properties of a cell	1422:1457	Variability in CSH could be an important characteristic in comparing the biological properties of cryptococcal strains.IMPORTANCE The interaction of a microbial cell with its environment is influenced by the biophysical properties of a cell.
32350094	2	75	theme	biophysical	273:283	arg1	parameter					285:293	a biophysical parameter	271:293	a biophysical parameter that influences the adhesion of fungal cells or spores to biotic and abiotic surfaces	271:379	Cell surface hydrophobicity (CSH) is a biophysical parameter that influences the adhesion of fungal cells or spores to biotic and abiotic surfaces.
32350094	2	75	theme	biophysical	273:283	arg1	hydrophobicity					247:260	Cell surface hydrophobicity	234:260	Cell surface hydrophobicity (CSH)	234:266	Cell surface hydrophobicity (CSH) is a biophysical parameter that influences the adhesion of fungal cells or spores to biotic and abiotic surfaces.
32350094	9	76	theme	cell	1480:1483	arg1	surface					1485:1491	the cell surface	1476:1491	the cell surface	1476:1491	The affinity of the cell surface for water, defined by the cell surface hydrophobicity (CSH), is a biophysical parameter that varies among different strains of Cryptococcus neoformans The CSH influences the phagocytosis of the yeast by its natural predator in the soil, the amoeba.
32350094	1	77	theme	pathogenic	169:178	arg1	gattii					158:163	Cryptococcus gattii	145:163	Cryptococcus gattii	145:163	Cryptococcus neoformans and Cryptococcus gattii are pathogenic fungi that cause significant morbidity and mortality.
32350094	1	77	theme	pathogenic	169:178	arg1	fungi					180:184	pathogenic fungi	169:184	pathogenic fungi that cause significant morbidity and mortality	169:231	Cryptococcus neoformans and Cryptococcus gattii are pathogenic fungi that cause significant morbidity and mortality.
32350094	1	77	theme	pathogenic	169:178	arg1	neoformans					130:139	Cryptococcus neoformans	117:139	Cryptococcus neoformans	117:139	Cryptococcus neoformans and Cryptococcus gattii are pathogenic fungi that cause significant morbidity and mortality.
32350094	5	78	theme	capsule	785:791	arg1	structure					768:776	higher-order structure	755:776	higher-order structure of the capsule	755:791	The capsular polysaccharides of C. neoformans strains differ in repeating motifs and therefore vary in the number of hydroxyl groups, which, along with higher-order structure of the capsule, may contribute to the variation in hydrophobicity that we observed.
32350094	8	79	from	Variability	1218:1228	arg1	CSH					1233:1235	CSH	1233:1235	CSH	1233:1235	Variability in CSH could be an important characteristic in comparing the biological properties of cryptococcal strains.IMPORTANCE The interaction of a microbial cell with its environment is influenced by the biophysical properties of a cell.
32350094	7	80	theme	strains	1209:1215	arg1	CSH					1188:1190	the CSH	1184:1190	the CSH of C. neoformans strains	1184:1215	For C. neoformans, CSH correlated with phagocytosis by natural soil predator Acanthamoeba castellanii Furthermore, capsular binding of the protective antibody (18B7), but not the nonprotective antibody (13F1), altered the CSH of C. neoformans strains.
32698596	0	0	theme	human	64:68	arg1	oligosaccharides					75:90	two human milk oligosaccharides	60:90	two human milk oligosaccharides	60:90	Real-world study in infants fed with an infant formula with two human milk oligosaccharides.
32698596	6	1	theme	formula	1219:1225	arg1	satisfaction					1227:1238	formula satisfaction	1219:1238	formula satisfaction	1219:1238	Secondary outcomes included formula satisfaction and adverse events (AEs).
32698596	1	2	theme	intestinal	224:233	arg1	microbiota					235:244	a balanced intestinal microbiota	213:244	a balanced intestinal microbiota	213:244	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	9	3	theme	group	1520:1524	arg1	differences					1526:1536	no significant group differences	1505:1536	no significant group differences at baseline, week 4, or week 8	1505:1567	Composite IGSQ scores demonstrated low GI distress in all groups, with no significant group differences at baseline, week 4, or week 8.
32698596	0	4	from	study	11:15	arg1	infants					20:26	infants	20:26	infants	20:26	Real-world study in infants fed with an infant formula with two human milk oligosaccharides.
32698596	9	5	theme	IGSQ	1444:1447	arg1	scores					1449:1454	Composite IGSQ scores	1434:1454	Composite IGSQ scores	1434:1454	Composite IGSQ scores demonstrated low GI distress in all groups, with no significant group differences at baseline, week 4, or week 8.
32698596	4	6	theme	milk-based	943:952	arg1	formula					954:960	a milk-based formula	941:960	a milk-based formula with 2' FL	941:971	Participants and methods: this open-label, prospective study was conducted at six centers in Spain, and included healthy, exclusively breastfed infants (BF group), an exclusively formula-fed group (FF) who received a milk-based formula with 2' FL and LNnT, and a group mixed fed with both formula and human milk (MF), for 8 weeks.
32698596	1	7	theme	microbiota	235:244	arg1	development					198:208	the development	194:208	the development of a balanced intestinal microbiota and immune protection in breastfed infants	194:287	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	4	8	dep	healthy	839:845	arg1	breastfed					860:868	breastfed	860:868	breastfed	860:868	Participants and methods: this open-label, prospective study was conducted at six centers in Spain, and included healthy, exclusively breastfed infants (BF group), an exclusively formula-fed group (FF) who received a milk-based formula with 2' FL and LNnT, and a group mixed fed with both formula and human milk (MF), for 8 weeks.
32698596	8	9	theme	Mean	1334:1337	arg1	z-scores					1339:1346	Mean z-scores	1334:1346	Mean z-scores for growth	1334:1357	Mean z-scores for growth were similar between all groups and within ± 0.5 of WHO medians at week 8.
32698596	11	10	theme	option	1821:1826	arg1	effectiveness					1785:1797	the effectiveness	1781:1797	the effectiveness of this early feeding option	1781:1826	Conclusions: in this RWE study examining a HMO-supplemented infant formula, growth and tolerance outcomes were similar to RCT findings, supporting the effectiveness of this early feeding option.
32698596	3	11	theme	Real-World	506:515	arg1	study					532:536	This Real-World Evidence (RWE) study	501:536	This Real-World Evidence (RWE) study	501:536	This Real-World Evidence (RWE) study aimed to evaluate growth and tolerance in infants consuming a formula supplemented with 1 g/L of 2'FL and 0.5 g/L of LNnT, and included a mixed-feeding group never studied before in RCTs.
32698596	0	12	theme	milk	70:73	arg1	oligosaccharides					75:90	two human milk oligosaccharides	60:90	two human milk oligosaccharides	60:90	Real-world study in infants fed with an infant formula with two human milk oligosaccharides.
32698596	4	13	with	formula	954:960	arg1	FL					970:971	2' FL	967:971	2' FL	967:971	Participants and methods: this open-label, prospective study was conducted at six centers in Spain, and included healthy, exclusively breastfed infants (BF group), an exclusively formula-fed group (FF) who received a milk-based formula with 2' FL and LNnT, and a group mixed fed with both formula and human milk (MF), for 8 weeks.
32698596	3	14	theme	Evidence	517:524	arg1	study					532:536	This Real-World Evidence (RWE) study	501:536	This Real-World Evidence (RWE) study	501:536	This Real-World Evidence (RWE) study aimed to evaluate growth and tolerance in infants consuming a formula supplemented with 1 g/L of 2'FL and 0.5 g/L of LNnT, and included a mixed-feeding group never studied before in RCTs.
32698596	1	15	theme	immune	250:255	arg1	protection					257:266	immune protection	250:266	immune protection	250:266	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	3	16	theme	LNnT	655:658	arg1	g/L					648:650	0.5 g/L	644:650	0.5 g/L of LNnT	644:658	This Real-World Evidence (RWE) study aimed to evaluate growth and tolerance in infants consuming a formula supplemented with 1 g/L of 2'FL and 0.5 g/L of LNnT, and included a mixed-feeding group never studied before in RCTs.
32698596	3	16	theme	LNnT	655:658	arg1	g/L					628:630	1 g/L	626:630	1 g/L of 2'FL	626:638	This Real-World Evidence (RWE) study aimed to evaluate growth and tolerance in infants consuming a formula supplemented with 1 g/L of 2'FL and 0.5 g/L of LNnT, and included a mixed-feeding group never studied before in RCTs.
32698596	4	17	theme	open-label	757:766	arg1	study					781:785	this open-label, prospective study	752:785	study	781:785	Participants and methods: this open-label, prospective study was conducted at six centers in Spain, and included healthy, exclusively breastfed infants (BF group), an exclusively formula-fed group (FF) who received a milk-based formula with 2' FL and LNnT, and a group mixed fed with both formula and human milk (MF), for 8 weeks.
32698596	0	18	with	formula	47:53	arg1	oligosaccharides					75:90	two human milk oligosaccharides	60:90	two human milk oligosaccharides	60:90	Real-world study in infants fed with an infant formula with two human milk oligosaccharides.
32698596	3	19	from	tolerance	567:575	arg1	infants					580:586	infants	580:586	infants consuming a formula supplemented with 1 g/L of 2'FL and 0.5 g/L of LNnT	580:658	This Real-World Evidence (RWE) study aimed to evaluate growth and tolerance in infants consuming a formula supplemented with 1 g/L of 2'FL and 0.5 g/L of LNnT, and included a mixed-feeding group never studied before in RCTs.
32698596	2	20	theme	HMOs	387:390	arg1	FL					413:414	2'FL	411:414	2'FL	411:414	Randomized controlled trials (RCTs) have demonstrated that infant formulas supplemented with the HMOs 2'-fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) are safe, well-tolerated, and support normal growth.
32698596	2	20	theme	HMOs	387:390	arg1	2'-fucosyllactose					392:408	the HMOs 2'-fucosyllactose	383:408	the HMOs 2'-fucosyllactose (2'FL)	383:415	Randomized controlled trials (RCTs) have demonstrated that infant formulas supplemented with the HMOs 2'-fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) are safe, well-tolerated, and support normal growth.
32698596	6	21	theme	adverse	1244:1250	arg1	AEs					1260:1262	AEs	1260:1262	AEs	1260:1262	Secondary outcomes included formula satisfaction and adverse events (AEs).
32698596	6	21	theme	adverse	1244:1250	arg1	events					1252:1257	adverse events	1244:1257	adverse events (AEs)	1244:1263	Secondary outcomes included formula satisfaction and adverse events (AEs).
32698596	9	22	from	week	1551:1554	arg1	differences					1526:1536	no significant group differences	1505:1536	no significant group differences at baseline, week 4, or week 8	1505:1567	Composite IGSQ scores demonstrated low GI distress in all groups, with no significant group differences at baseline, week 4, or week 8.
32698596	9	23	theme	Composite	1434:1442	arg1	scores					1449:1454	Composite IGSQ scores	1434:1454	Composite IGSQ scores	1434:1454	Composite IGSQ scores demonstrated low GI distress in all groups, with no significant group differences at baseline, week 4, or week 8.
32698596	8	24	from	week	1426:1429	arg1	medians					1415:1421	medians	1415:1421	medians	1415:1421	Mean z-scores for growth were similar between all groups and within ± 0.5 of WHO medians at week 8.
32698596	8	24	from	week	1426:1429	arg1	±					1402:1402	± 0.5	1402:1406	± 0.5 of WHO medians at week 8	1402:1431	Mean z-scores for growth were similar between all groups and within ± 0.5 of WHO medians at week 8.
32698596	1	25	theme	important	149:157	arg1	component					159:167	an important component	146:167	an important component of human milk	146:181	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	1	25	theme	important	149:157	arg1	oligosaccharides					118:133	human milk oligosaccharides	107:133	human milk oligosaccharides (HMOs)	107:140	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	1	25	theme	important	149:157	arg1	Introduction					93:104	Introduction	93:104	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.	93:288	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	4	26	theme	prospective	769:779	arg1	study					781:785	this open-label, prospective study	752:785	study	781:785	Participants and methods: this open-label, prospective study was conducted at six centers in Spain, and included healthy, exclusively breastfed infants (BF group), an exclusively formula-fed group (FF) who received a milk-based formula with 2' FL and LNnT, and a group mixed fed with both formula and human milk (MF), for 8 weeks.
32698596	7	27	dep	study	1301:1305	arg1	MF					1318:1319	48 MF	1315:1319	48 MF	1315:1319	Results: 159 infants completed the study (66 FF, 48 MF, and 45 BF).
32698596	7	27	dep	study	1301:1305	arg1	BF					1329:1330	45 BF	1326:1330	45 BF	1326:1330	Results: 159 infants completed the study (66 FF, 48 MF, and 45 BF).
32698596	7	27	dep	study	1301:1305	arg1	FF					1311:1312	66 FF	1308:1312	66 FF	1308:1312	Results: 159 infants completed the study (66 FF, 48 MF, and 45 BF).
32698596	11	28	theme	RCT	1756:1758	arg1	findings					1760:1767	RCT findings	1756:1767	RCT findings	1756:1767	Conclusions: in this RWE study examining a HMO-supplemented infant formula, growth and tolerance outcomes were similar to RCT findings, supporting the effectiveness of this early feeding option.
32698596	11	29	theme	feeding	1813:1819	arg1	option					1821:1826	this early feeding option	1802:1826	this early feeding option	1802:1826	Conclusions: in this RWE study examining a HMO-supplemented infant formula, growth and tolerance outcomes were similar to RCT findings, supporting the effectiveness of this early feeding option.
32698596	0	30	theme	Real-world	0:9	arg1	study					11:15	Real-world study	0:15	Real-world study in infants	0:26	Real-world study in infants fed with an infant formula with two human milk oligosaccharides.
32698596	1	31	theme	protection	257:266	arg1	development					198:208	the development	194:208	the development of a balanced intestinal microbiota and immune protection in breastfed infants	194:287	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	2	32	theme	controlled	301:310	arg1	RCTs					320:323	RCTs	320:323	RCTs	320:323	Randomized controlled trials (RCTs) have demonstrated that infant formulas supplemented with the HMOs 2'-fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) are safe, well-tolerated, and support normal growth.
32698596	2	32	theme	controlled	301:310	arg1	trials					312:317	Randomized controlled trials	290:317	Randomized controlled trials (RCTs)	290:324	Randomized controlled trials (RCTs) have demonstrated that infant formulas supplemented with the HMOs 2'-fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) are safe, well-tolerated, and support normal growth.
32698596	11	33	from	study	1659:1663	arg1	Conclusions					1634:1644	Conclusions	1634:1644	Conclusions: in this RWE study examining a HMO-supplemented infant formula, growth and tolerance outcomes were similar to RCT findings, supporting the effectiveness of this early feeding option.	1634:1827	Conclusions: in this RWE study examining a HMO-supplemented infant formula, growth and tolerance outcomes were similar to RCT findings, supporting the effectiveness of this early feeding option.
32698596	11	33	from	study	1659:1663	arg1	similar					1745:1751	similar	1745:1751	similar	1745:1751	Conclusions: in this RWE study examining a HMO-supplemented infant formula, growth and tolerance outcomes were similar to RCT findings, supporting the effectiveness of this early feeding option.
32698596	2	34	theme	Randomized	290:299	arg1	RCTs					320:323	RCTs	320:323	RCTs	320:323	Randomized controlled trials (RCTs) have demonstrated that infant formulas supplemented with the HMOs 2'-fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) are safe, well-tolerated, and support normal growth.
32698596	2	34	theme	Randomized	290:299	arg1	trials					312:317	Randomized controlled trials	290:317	Randomized controlled trials (RCTs)	290:324	Randomized controlled trials (RCTs) have demonstrated that infant formulas supplemented with the HMOs 2'-fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) are safe, well-tolerated, and support normal growth.
32698596	1	35	theme	human	172:176	arg1	milk					178:181	human milk	172:181	human milk	172:181	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	1	36	theme	breastfed	271:279	arg1	infants					281:287	breastfed infants	271:287	breastfed infants	271:287	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	9	37	theme	low	1469:1471	arg1	distress					1476:1483	low GI distress	1469:1483	low GI distress	1469:1483	Composite IGSQ scores demonstrated low GI distress in all groups, with no significant group differences at baseline, week 4, or week 8.
32698596	9	38	from	baseline	1541:1548	arg1	differences					1526:1536	no significant group differences	1505:1536	no significant group differences at baseline, week 4, or week 8	1505:1567	Composite IGSQ scores demonstrated low GI distress in all groups, with no significant group differences at baseline, week 4, or week 8.
32698596	3	39	theme	RWE	527:529	arg1	study					532:536	This Real-World Evidence (RWE) study	501:536	This Real-World Evidence (RWE) study	501:536	This Real-World Evidence (RWE) study aimed to evaluate growth and tolerance in infants consuming a formula supplemented with 1 g/L of 2'FL and 0.5 g/L of LNnT, and included a mixed-feeding group never studied before in RCTs.
32698596	1	40	theme	milk	178:181	arg1	component					159:167	an important component	146:167	an important component of human milk	146:181	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	1	40	theme	milk	178:181	arg1	oligosaccharides					118:133	human milk oligosaccharides	107:133	human milk oligosaccharides (HMOs)	107:140	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	1	40	theme	milk	178:181	arg1	Introduction					93:104	Introduction	93:104	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.	93:288	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	9	41	theme	GI	1473:1474	arg1	distress					1476:1483	low GI distress	1469:1483	low GI distress	1469:1483	Composite IGSQ scores demonstrated low GI distress in all groups, with no significant group differences at baseline, week 4, or week 8.
32698596	11	42	theme	tolerance	1721:1729	arg1	outcomes					1731:1738	growth and tolerance outcomes	1710:1738	growth and tolerance outcomes	1710:1738	Conclusions: in this RWE study examining a HMO-supplemented infant formula, growth and tolerance outcomes were similar to RCT findings, supporting the effectiveness of this early feeding option.
32698596	3	43	from	growth	556:561	arg1	infants					580:586	infants	580:586	infants consuming a formula supplemented with 1 g/L of 2'FL and 0.5 g/L of LNnT	580:658	This Real-World Evidence (RWE) study aimed to evaluate growth and tolerance in infants consuming a formula supplemented with 1 g/L of 2'FL and 0.5 g/L of LNnT, and included a mixed-feeding group never studied before in RCTs.
32698596	10	44	theme	AEs	1583:1585	arg1	Incidence					1570:1578	Incidence	1570:1578	Incidence of AEs	1570:1585	Incidence of AEs was low overall, and comparable across groups.
32698596	11	45	theme	HMO-supplemented	1677:1692	arg1	formula					1701:1707	a HMO-supplemented infant formula	1675:1707	a HMO-supplemented infant formula	1675:1707	Conclusions: in this RWE study examining a HMO-supplemented infant formula, growth and tolerance outcomes were similar to RCT findings, supporting the effectiveness of this early feeding option.
32698596	4	46	theme	human	1027:1031	arg1	MF					1039:1040	MF	1039:1040	MF	1039:1040	Participants and methods: this open-label, prospective study was conducted at six centers in Spain, and included healthy, exclusively breastfed infants (BF group), an exclusively formula-fed group (FF) who received a milk-based formula with 2' FL and LNnT, and a group mixed fed with both formula and human milk (MF), for 8 weeks.
32698596	4	46	theme	human	1027:1031	arg1	milk					1033:1036	human milk	1027:1036	human milk (MF)	1027:1041	Participants and methods: this open-label, prospective study was conducted at six centers in Spain, and included healthy, exclusively breastfed infants (BF group), an exclusively formula-fed group (FF) who received a milk-based formula with 2' FL and LNnT, and a group mixed fed with both formula and human milk (MF), for 8 weeks.
32698596	4	47	dep	Participants	726:737	arg1	conducted					791:799	conducted	791:799	was conducted at six centers in Spain	787:823	Participants and methods: this open-label, prospective study was conducted at six centers in Spain, and included healthy, exclusively breastfed infants (BF group), an exclusively formula-fed group (FF) who received a milk-based formula with 2' FL and LNnT, and a group mixed fed with both formula and human milk (MF), for 8 weeks.
32698596	4	47	dep	Participants	726:737	arg1	included					830:837	included	830:837	included healthy, exclusively breastfed infants (BF group), an exclusively formula-fed group (FF) who received a milk-based formula with 2' FL and LNnT, and a group mixed fed with both formula and human milk (MF), for 8 weeks	830:1054	Participants and methods: this open-label, prospective study was conducted at six centers in Spain, and included healthy, exclusively breastfed infants (BF group), an exclusively formula-fed group (FF) who received a milk-based formula with 2' FL and LNnT, and a group mixed fed with both formula and human milk (MF), for 8 weeks.
32698596	0	48	theme	infant	40:45	arg1	formula					47:53	an infant formula	37:53	an infant formula with two human milk oligosaccharides	37:90	Real-world study in infants fed with an infant formula with two human milk oligosaccharides.
32698596	11	49	theme	RWE	1655:1657	arg1	study					1659:1663	this RWE study	1650:1663	this RWE study examining a HMO-supplemented infant formula	1650:1707	Conclusions: in this RWE study examining a HMO-supplemented infant formula, growth and tolerance outcomes were similar to RCT findings, supporting the effectiveness of this early feeding option.
32698596	11	50	theme	early	1807:1811	arg1	option					1821:1826	this early feeding option	1802:1826	this early feeding option	1802:1826	Conclusions: in this RWE study examining a HMO-supplemented infant formula, growth and tolerance outcomes were similar to RCT findings, supporting the effectiveness of this early feeding option.
32698596	5	51	theme	Co-primary	1057:1066	arg1	outcomes					1068:1075	Co-primary outcomes	1057:1075	Co-primary outcomes	1057:1075	Co-primary outcomes were growth (anthropometry) and gastrointestinal tolerance (Infant Gastrointestinal Symptom Questionnaire, IGSQ).
32698596	5	52	theme	gastrointestinal	1109:1124	arg1	tolerance					1126:1134	gastrointestinal tolerance	1109:1134	gastrointestinal tolerance (Infant Gastrointestinal Symptom Questionnaire, IGSQ)	1109:1188	Co-primary outcomes were growth (anthropometry) and gastrointestinal tolerance (Infant Gastrointestinal Symptom Questionnaire, IGSQ).
32698596	3	53	theme	mixed-feeding	676:688	arg1	group					690:694	a mixed-feeding group	674:694	a mixed-feeding group	674:694	This Real-World Evidence (RWE) study aimed to evaluate growth and tolerance in infants consuming a formula supplemented with 1 g/L of 2'FL and 0.5 g/L of LNnT, and included a mixed-feeding group never studied before in RCTs.
32698596	2	54	dep	safe	452:455	arg1	well-tolerated					458:471	well-tolerated	458:471	well-tolerated	458:471	Randomized controlled trials (RCTs) have demonstrated that infant formulas supplemented with the HMOs 2'-fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) are safe, well-tolerated, and support normal growth.
32698596	1	55	theme	human	107:111	arg1	component					159:167	an important component	146:167	an important component of human milk	146:181	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	1	55	theme	human	107:111	arg1	HMOs					136:139	HMOs	136:139	HMOs	136:139	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	1	55	theme	human	107:111	arg1	oligosaccharides					118:133	human milk oligosaccharides	107:133	human milk oligosaccharides (HMOs)	107:140	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	1	55	theme	human	107:111	arg1	Introduction					93:104	Introduction	93:104	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.	93:288	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	11	56	from	similar	1745:1751	arg1	study					1659:1663	this RWE study	1650:1663	this RWE study examining a HMO-supplemented infant formula	1650:1707	Conclusions: in this RWE study examining a HMO-supplemented infant formula, growth and tolerance outcomes were similar to RCT findings, supporting the effectiveness of this early feeding option.
32698596	1	57	from	development	198:208	arg1	infants					281:287	breastfed infants	271:287	breastfed infants	271:287	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	1	58	theme	milk	113:116	arg1	component					159:167	an important component	146:167	an important component of human milk	146:181	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	1	58	theme	milk	113:116	arg1	HMOs					136:139	HMOs	136:139	HMOs	136:139	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	1	58	theme	milk	113:116	arg1	oligosaccharides					118:133	human milk oligosaccharides	107:133	human milk oligosaccharides (HMOs)	107:140	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	1	58	theme	milk	113:116	arg1	Introduction					93:104	Introduction	93:104	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.	93:288	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	8	59	theme	medians	1415:1421	arg1	±					1402:1402	± 0.5	1402:1406	± 0.5 of WHO medians at week 8	1402:1431	Mean z-scores for growth were similar between all groups and within ± 0.5 of WHO medians at week 8.
32698596	9	60	theme	significant	1508:1518	arg1	differences					1526:1536	no significant group differences	1505:1536	no significant group differences at baseline, week 4, or week 8	1505:1567	Composite IGSQ scores demonstrated low GI distress in all groups, with no significant group differences at baseline, week 4, or week 8.
32698596	8	61	from	±	1402:1402	arg1	week					1426:1429	week 8	1426:1431	week 8	1426:1431	Mean z-scores for growth were similar between all groups and within ± 0.5 of WHO medians at week 8.
32698596	4	62	from	centers	808:814	arg1	Spain					819:823	Spain	819:823	Spain	819:823	Participants and methods: this open-label, prospective study was conducted at six centers in Spain, and included healthy, exclusively breastfed infants (BF group), an exclusively formula-fed group (FF) who received a milk-based formula with 2' FL and LNnT, and a group mixed fed with both formula and human milk (MF), for 8 weeks.
32698596	11	63	theme	growth	1710:1715	arg1	outcomes					1731:1738	growth and tolerance outcomes	1710:1738	growth and tolerance outcomes	1710:1738	Conclusions: in this RWE study examining a HMO-supplemented infant formula, growth and tolerance outcomes were similar to RCT findings, supporting the effectiveness of this early feeding option.
32698596	9	64	from	week	1562:1565	arg1	differences					1526:1536	no significant group differences	1505:1536	no significant group differences at baseline, week 4, or week 8	1505:1567	Composite IGSQ scores demonstrated low GI distress in all groups, with no significant group differences at baseline, week 4, or week 8.
32698596	6	65	theme	Secondary	1191:1199	arg1	outcomes					1201:1208	Secondary outcomes	1191:1208	Secondary outcomes	1191:1208	Secondary outcomes included formula satisfaction and adverse events (AEs).
32698596	4	66	theme	BF	879:880	arg1	infants					870:876	infants	870:876	infants (BF group)	870:887	Participants and methods: this open-label, prospective study was conducted at six centers in Spain, and included healthy, exclusively breastfed infants (BF group), an exclusively formula-fed group (FF) who received a milk-based formula with 2' FL and LNnT, and a group mixed fed with both formula and human milk (MF), for 8 weeks.
32698596	4	66	theme	BF	879:880	arg1	group					882:886	BF group	879:886	BF group	879:886	Participants and methods: this open-label, prospective study was conducted at six centers in Spain, and included healthy, exclusively breastfed infants (BF group), an exclusively formula-fed group (FF) who received a milk-based formula with 2' FL and LNnT, and a group mixed fed with both formula and human milk (MF), for 8 weeks.
32698596	11	67	theme	infant	1694:1699	arg1	formula					1701:1707	a HMO-supplemented infant formula	1675:1707	a HMO-supplemented infant formula	1675:1707	Conclusions: in this RWE study examining a HMO-supplemented infant formula, growth and tolerance outcomes were similar to RCT findings, supporting the effectiveness of this early feeding option.
32698596	11	68	dep	similar	1745:1751	arg1	supporting					1770:1779	supporting	1770:1779	supporting the effectiveness of this early feeding option	1770:1826	Conclusions: in this RWE study examining a HMO-supplemented infant formula, growth and tolerance outcomes were similar to RCT findings, supporting the effectiveness of this early feeding option.
32698596	3	69	dep	included	665:672	arg1	studied					702:708	studied	702:708	included a mixed-feeding group never studied before in RCTs	665:723	This Real-World Evidence (RWE) study aimed to evaluate growth and tolerance in infants consuming a formula supplemented with 1 g/L of 2'FL and 0.5 g/L of LNnT, and included a mixed-feeding group never studied before in RCTs.
32698596	4	70	theme	formula-fed	905:915	arg1	FF					924:925	FF	924:925	FF	924:925	Participants and methods: this open-label, prospective study was conducted at six centers in Spain, and included healthy, exclusively breastfed infants (BF group), an exclusively formula-fed group (FF) who received a milk-based formula with 2' FL and LNnT, and a group mixed fed with both formula and human milk (MF), for 8 weeks.
32698596	4	70	theme	formula-fed	905:915	arg1	group					917:921	an exclusively formula-fed group	890:921	an exclusively formula-fed group (FF) who received a milk-based formula with 2' FL	890:971	Participants and methods: this open-label, prospective study was conducted at six centers in Spain, and included healthy, exclusively breastfed infants (BF group), an exclusively formula-fed group (FF) who received a milk-based formula with 2' FL and LNnT, and a group mixed fed with both formula and human milk (MF), for 8 weeks.
32698596	3	71	theme	FL	637:638	arg1	g/L					648:650	0.5 g/L	644:650	0.5 g/L of LNnT	644:658	This Real-World Evidence (RWE) study aimed to evaluate growth and tolerance in infants consuming a formula supplemented with 1 g/L of 2'FL and 0.5 g/L of LNnT, and included a mixed-feeding group never studied before in RCTs.
32698596	3	71	theme	FL	637:638	arg1	g/L					628:630	1 g/L	626:630	1 g/L of 2'FL	626:638	This Real-World Evidence (RWE) study aimed to evaluate growth and tolerance in infants consuming a formula supplemented with 1 g/L of 2'FL and 0.5 g/L of LNnT, and included a mixed-feeding group never studied before in RCTs.
32698596	1	72	theme	balanced	215:222	arg1	microbiota					235:244	a balanced intestinal microbiota	213:244	a balanced intestinal microbiota	213:244	Introduction: human milk oligosaccharides (HMOs) are an important component of human milk supporting the development of a balanced intestinal microbiota and immune protection in breastfed infants.
32698596	5	73	dep	tolerance	1126:1134	arg1	Gastrointestinal					1144:1159	Gastrointestinal	1144:1159	Gastrointestinal	1144:1159	Co-primary outcomes were growth (anthropometry) and gastrointestinal tolerance (Infant Gastrointestinal Symptom Questionnaire, IGSQ).
32698596	2	74	theme	infant	349:354	arg1	formulas					356:363	infant formulas	349:363	infant formulas supplemented with the HMOs 2'-fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT)	349:446	Randomized controlled trials (RCTs) have demonstrated that infant formulas supplemented with the HMOs 2'-fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) are safe, well-tolerated, and support normal growth.
32698596	2	75	theme	normal	486:491	arg1	growth					493:498	normal growth	486:498	normal growth	486:498	Randomized controlled trials (RCTs) have demonstrated that infant formulas supplemented with the HMOs 2'-fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) are safe, well-tolerated, and support normal growth.
34146974	3	0	from	maximization	562:573	arg1	phenolics					597:605	total phenolics	591:605	total phenolics using response surface methodology	591:640	Under this framework, the ultrasound-assisted extraction (UAE) of polyphenols from carob pulp was optimized by the maximization of the yield in total phenolics using response surface methodology.
34146974	1	1	theme	promoting	272:280	arg1	effects					282:288	biological and health promoting effects	250:288	biological and health promoting effects	250:288	Polyphenols in carobs have recently attracted great attention due to their wide range of biological and health promoting effects.
34146974	0	2	theme	Processing	78:87	arg1	parameters					89:98	Processing parameters optimization and identification	78:130	Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.	0:159	Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.
34146974	0	2	theme	Processing	78:87	arg1	identification					117:130	identification	117:130	identification	117:130	Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.
34146974	0	2	theme	Processing	78:87	arg1	optimization					100:111	optimization	100:111	optimization	100:111	Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.
34146974	7	3	from	influence	1197:1205	arg1	composition					1239:1249	carobs composition	1232:1249	carobs composition	1232:1249	The results from the analysis of real samples revealed the predominance of gallic acid and highlighted the great influence of the ripening stage on carobs composition.
34146974	3	4	from	pulp	536:539	arg1	UAE					505:507	UAE	505:507	UAE	505:507	Under this framework, the ultrasound-assisted extraction (UAE) of polyphenols from carob pulp was optimized by the maximization of the yield in total phenolics using response surface methodology.
34146974	3	4	from	pulp	536:539	arg1	polyphenols					513:523	polyphenols	513:523	polyphenols from carob pulp	513:539	Under this framework, the ultrasound-assisted extraction (UAE) of polyphenols from carob pulp was optimized by the maximization of the yield in total phenolics using response surface methodology.
34146974	3	4	from	pulp	536:539	arg1	extraction					493:502	the ultrasound-assisted extraction	469:502	the ultrasound-assisted extraction (UAE) of polyphenols from carob pulp	469:539	Under this framework, the ultrasound-assisted extraction (UAE) of polyphenols from carob pulp was optimized by the maximization of the yield in total phenolics using response surface methodology.
34146974	4	5	theme	time	728:731	arg1	effects					662:668	the effects	658:668	the effects of solid-solvent ratio, solvent concentration, extraction time, sonication amplitude, and sonication mode	658:774	In particular, the effects of solid-solvent ratio, solvent concentration, extraction time, sonication amplitude, and sonication mode were investigated and optimized using a complete experimental design.
34146974	1	6	theme	effects	282:288	arg1	range					241:245	their wide range	230:245	their wide range of biological and health promoting effects	230:288	Polyphenols in carobs have recently attracted great attention due to their wide range of biological and health promoting effects.
34146974	7	7	theme	gallic	1159:1164	arg1	acid					1166:1169	gallic acid	1159:1169	gallic acid	1159:1169	The results from the analysis of real samples revealed the predominance of gallic acid and highlighted the great influence of the ripening stage on carobs composition.
34146974	2	8	theme	optimum	338:344	arg1	method					346:351	an optimum method	335:351	an optimum method for the extraction, purification and characterization of these valuable bioactive substances	335:444	A comprehensive study was conducted to find an optimum method for the extraction, purification and characterization of these valuable bioactive substances.
34146974	7	9	theme	acid	1166:1169	arg1	predominance					1143:1154	the predominance	1139:1154	the predominance of gallic acid	1139:1169	The results from the analysis of real samples revealed the predominance of gallic acid and highlighted the great influence of the ripening stage on carobs composition.
34146974	6	10	theme	prior	1033:1037	arg1	extraction					985:994	Solid-phase extraction	973:994	Solid-phase extraction	973:994	Solid-phase extraction was evaluated as a clean-up strategy prior to the electrophoretic analysis of extracts.
34146974	6	10	theme	prior	1033:1037	arg1	strategy					1024:1031	a clean-up strategy	1013:1031	a clean-up strategy prior to the electrophoretic analysis of extracts	1013:1081	Solid-phase extraction was evaluated as a clean-up strategy prior to the electrophoretic analysis of extracts.
34146974	7	11	theme	carobs	1232:1237	arg1	composition					1239:1249	carobs composition	1232:1249	carobs composition	1232:1249	The results from the analysis of real samples revealed the predominance of gallic acid and highlighted the great influence of the ripening stage on carobs composition.
34146974	5	12	theme	shorter	948:954	arg1	time					967:970	a shorter processing time	946:970	a shorter processing time	946:970	In comparison to conventional extraction techniques, UAE offered a higher yield of antioxidants and a shorter processing time.
34146974	7	13	theme	ripening	1214:1221	arg1	stage					1223:1227	the ripening stage	1210:1227	the ripening stage	1210:1227	The results from the analysis of real samples revealed the predominance of gallic acid and highlighted the great influence of the ripening stage on carobs composition.
34146974	4	14	theme	mode	771:774	arg1	effects					662:668	the effects	658:668	the effects of solid-solvent ratio, solvent concentration, extraction time, sonication amplitude, and sonication mode	658:774	In particular, the effects of solid-solvent ratio, solvent concentration, extraction time, sonication amplitude, and sonication mode were investigated and optimized using a complete experimental design.
34146974	5	15	theme	higher	913:918	arg1	yield					920:924	a higher yield	911:924	a higher yield of antioxidants	911:940	In comparison to conventional extraction techniques, UAE offered a higher yield of antioxidants and a shorter processing time.
34146974	2	16	theme	bioactive	425:433	arg1	substances					435:444	these valuable bioactive substances	410:444	these valuable bioactive substances	410:444	A comprehensive study was conducted to find an optimum method for the extraction, purification and characterization of these valuable bioactive substances.
34146974	6	17	theme	electrophoretic	1046:1060	arg1	analysis					1062:1069	the electrophoretic analysis	1042:1069	the electrophoretic analysis of extracts	1042:1081	Solid-phase extraction was evaluated as a clean-up strategy prior to the electrophoretic analysis of extracts.
34146974	7	18	theme	samples	1122:1128	arg1	analysis					1105:1112	the analysis	1101:1112	the analysis of real samples	1101:1128	The results from the analysis of real samples revealed the predominance of gallic acid and highlighted the great influence of the ripening stage on carobs composition.
34146974	2	19	theme	comprehensive	293:305	arg1	study					307:311	A comprehensive study	291:311	A comprehensive study	291:311	A comprehensive study was conducted to find an optimum method for the extraction, purification and characterization of these valuable bioactive substances.
34146974	1	20	theme	great	207:211	arg1	attention					213:221	great attention	207:221	great attention	207:221	Polyphenols in carobs have recently attracted great attention due to their wide range of biological and health promoting effects.
34146974	7	21	theme	stage	1223:1227	arg1	influence					1197:1205	the great influence	1187:1205	the great influence of the ripening stage on carobs composition	1187:1249	The results from the analysis of real samples revealed the predominance of gallic acid and highlighted the great influence of the ripening stage on carobs composition.
34146974	1	22	from	Polyphenols	161:171	arg1	carobs					176:181	carobs	176:181	carobs	176:181	Polyphenols in carobs have recently attracted great attention due to their wide range of biological and health promoting effects.
34146974	0	23	dep	parameters	89:98	arg1	parameters					89:98	Processing parameters optimization and identification	78:130	Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.	0:159	Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.
34146974	0	23	dep	parameters	89:98	arg1	identification					117:130	identification	117:130	identification	117:130	Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.
34146974	0	23	dep	parameters	89:98	arg1	optimization					100:111	optimization	100:111	optimization	100:111	Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.
34146974	4	24	theme	concentration	702:714	arg1	effects					662:668	the effects	658:668	the effects of solid-solvent ratio, solvent concentration, extraction time, sonication amplitude, and sonication mode	658:774	In particular, the effects of solid-solvent ratio, solvent concentration, extraction time, sonication amplitude, and sonication mode were investigated and optimized using a complete experimental design.
34146974	0	25	theme	Continuous	0:9	arg1	extraction					42:51	Continuous and pulsed ultrasound-assisted extraction	0:51	extraction	42:51	Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.
34146974	6	26	theme	Solid-phase	973:983	arg1	extraction					985:994	Solid-phase extraction	973:994	Solid-phase extraction	973:994	Solid-phase extraction was evaluated as a clean-up strategy prior to the electrophoretic analysis of extracts.
34146974	6	26	theme	Solid-phase	973:983	arg1	strategy					1024:1031	a clean-up strategy	1013:1031	a clean-up strategy prior to the electrophoretic analysis of extracts	1013:1081	Solid-phase extraction was evaluated as a clean-up strategy prior to the electrophoretic analysis of extracts.
34146974	4	27	theme	sonication	760:769	arg1	mode					771:774	sonication mode	760:774	sonication mode	760:774	In particular, the effects of solid-solvent ratio, solvent concentration, extraction time, sonication amplitude, and sonication mode were investigated and optimized using a complete experimental design.
34146974	4	28	theme	solvent	694:700	arg1	concentration					702:714	solvent concentration	694:714	solvent concentration	694:714	In particular, the effects of solid-solvent ratio, solvent concentration, extraction time, sonication amplitude, and sonication mode were investigated and optimized using a complete experimental design.
34146974	0	29	theme	ultrasound-assisted	22:40	arg1	extraction					42:51	Continuous and pulsed ultrasound-assisted extraction	0:51	extraction	42:51	Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.
34146974	6	30	theme	clean-up	1015:1022	arg1	extraction					985:994	Solid-phase extraction	973:994	Solid-phase extraction	973:994	Solid-phase extraction was evaluated as a clean-up strategy prior to the electrophoretic analysis of extracts.
34146974	6	30	theme	clean-up	1015:1022	arg1	strategy					1024:1031	a clean-up strategy	1013:1031	a clean-up strategy prior to the electrophoretic analysis of extracts	1013:1081	Solid-phase extraction was evaluated as a clean-up strategy prior to the electrophoretic analysis of extracts.
34146974	0	31	theme	composition	148:158	arg1	parameters					89:98	Processing parameters optimization and identification	78:130	Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.	0:159	Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.
34146974	0	31	theme	composition	148:158	arg1	identification					117:130	identification	117:130	identification	117:130	Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.
34146974	0	31	theme	composition	148:158	arg1	optimization					100:111	optimization	100:111	optimization	100:111	Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.
34146974	0	32	dep	extraction	42:51	arg1	parameters					89:98	Processing parameters optimization and identification	78:130	Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.	0:159	Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.
34146974	0	32	dep	extraction	42:51	arg1	identification					117:130	identification	117:130	identification	117:130	Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.
34146974	0	32	dep	extraction	42:51	arg1	optimization					100:111	optimization	100:111	optimization	100:111	Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.
34146974	3	33	theme	yield	582:586	arg1	maximization					562:573	the maximization	558:573	the maximization of the yield in total phenolics using response surface methodology	558:640	Under this framework, the ultrasound-assisted extraction (UAE) of polyphenols from carob pulp was optimized by the maximization of the yield in total phenolics using response surface methodology.
34146974	5	34	theme	extraction	876:885	arg1	techniques					887:896	conventional extraction techniques	863:896	conventional extraction techniques	863:896	In comparison to conventional extraction techniques, UAE offered a higher yield of antioxidants and a shorter processing time.
34146974	0	35	theme	pulsed	15:20	arg1	extraction					42:51	Continuous and pulsed ultrasound-assisted extraction	0:51	extraction	42:51	Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.
34146974	0	36	theme	polyphenolic	135:146	arg1	composition					148:158	polyphenolic composition	135:158	polyphenolic composition	135:158	Continuous and pulsed ultrasound-assisted extraction of carob's antioxidants: Processing parameters optimization and identification of polyphenolic composition.
34146974	4	37	theme	ratio	687:691	arg1	effects					662:668	the effects	658:668	the effects of solid-solvent ratio, solvent concentration, extraction time, sonication amplitude, and sonication mode	658:774	In particular, the effects of solid-solvent ratio, solvent concentration, extraction time, sonication amplitude, and sonication mode were investigated and optimized using a complete experimental design.
34146974	4	38	theme	sonication	734:743	arg1	amplitude					745:753	sonication amplitude	734:753	sonication amplitude	734:753	In particular, the effects of solid-solvent ratio, solvent concentration, extraction time, sonication amplitude, and sonication mode were investigated and optimized using a complete experimental design.
34146974	4	39	theme	solid-solvent	673:685	arg1	ratio					687:691	solid-solvent ratio	673:691	solid-solvent ratio	673:691	In particular, the effects of solid-solvent ratio, solvent concentration, extraction time, sonication amplitude, and sonication mode were investigated and optimized using a complete experimental design.
34146974	3	40	theme	surface	622:628	arg1	methodology					630:640	response surface methodology	613:640	response surface methodology	613:640	Under this framework, the ultrasound-assisted extraction (UAE) of polyphenols from carob pulp was optimized by the maximization of the yield in total phenolics using response surface methodology.
34146974	1	41	theme	wide	236:239	arg1	range					241:245	their wide range	230:245	their wide range of biological and health promoting effects	230:288	Polyphenols in carobs have recently attracted great attention due to their wide range of biological and health promoting effects.
34146974	5	42	theme	processing	956:965	arg1	time					967:970	a shorter processing time	946:970	a shorter processing time	946:970	In comparison to conventional extraction techniques, UAE offered a higher yield of antioxidants and a shorter processing time.
34146974	3	43	from	extraction	493:502	arg1	pulp					536:539	carob pulp	530:539	carob pulp	530:539	Under this framework, the ultrasound-assisted extraction (UAE) of polyphenols from carob pulp was optimized by the maximization of the yield in total phenolics using response surface methodology.
34146974	2	44	theme	substances	435:444	arg1	purification					373:384	purification	373:384	purification	373:384	A comprehensive study was conducted to find an optimum method for the extraction, purification and characterization of these valuable bioactive substances.
34146974	2	44	theme	substances	435:444	arg1	extraction					361:370	extraction	361:370	extraction	361:370	A comprehensive study was conducted to find an optimum method for the extraction, purification and characterization of these valuable bioactive substances.
34146974	2	44	theme	substances	435:444	arg1	characterization					390:405	characterization	390:405	characterization	390:405	A comprehensive study was conducted to find an optimum method for the extraction, purification and characterization of these valuable bioactive substances.
34146974	4	45	theme	amplitude	745:753	arg1	effects					662:668	the effects	658:668	the effects of solid-solvent ratio, solvent concentration, extraction time, sonication amplitude, and sonication mode	658:774	In particular, the effects of solid-solvent ratio, solvent concentration, extraction time, sonication amplitude, and sonication mode were investigated and optimized using a complete experimental design.
34146974	7	46	theme	great	1191:1195	arg1	influence					1197:1205	the great influence	1187:1205	the great influence of the ripening stage on carobs composition	1187:1249	The results from the analysis of real samples revealed the predominance of gallic acid and highlighted the great influence of the ripening stage on carobs composition.
34146974	7	47	theme	real	1117:1120	arg1	samples					1122:1128	real samples	1117:1128	real samples	1117:1128	The results from the analysis of real samples revealed the predominance of gallic acid and highlighted the great influence of the ripening stage on carobs composition.
34146974	3	48	theme	response	613:620	arg1	methodology					630:640	response surface methodology	613:640	response surface methodology	613:640	Under this framework, the ultrasound-assisted extraction (UAE) of polyphenols from carob pulp was optimized by the maximization of the yield in total phenolics using response surface methodology.
34146974	3	49	theme	carob	530:534	arg1	pulp					536:539	carob pulp	530:539	carob pulp	530:539	Under this framework, the ultrasound-assisted extraction (UAE) of polyphenols from carob pulp was optimized by the maximization of the yield in total phenolics using response surface methodology.
34146974	3	50	theme	total	591:595	arg1	phenolics					597:605	total phenolics	591:605	total phenolics using response surface methodology	591:640	Under this framework, the ultrasound-assisted extraction (UAE) of polyphenols from carob pulp was optimized by the maximization of the yield in total phenolics using response surface methodology.
34146974	4	51	theme	experimental	825:836	arg1	design					838:843	a complete experimental design	814:843	a complete experimental design	814:843	In particular, the effects of solid-solvent ratio, solvent concentration, extraction time, sonication amplitude, and sonication mode were investigated and optimized using a complete experimental design.
34146974	6	52	theme	extracts	1074:1081	arg1	analysis					1062:1069	the electrophoretic analysis	1042:1069	the electrophoretic analysis of extracts	1042:1081	Solid-phase extraction was evaluated as a clean-up strategy prior to the electrophoretic analysis of extracts.
34146974	1	53	dep	promoting	272:280	arg1	health					265:270	health	265:270	health	265:270	Polyphenols in carobs have recently attracted great attention due to their wide range of biological and health promoting effects.
34146974	4	54	theme	complete	816:823	arg1	design					838:843	a complete experimental design	814:843	a complete experimental design	814:843	In particular, the effects of solid-solvent ratio, solvent concentration, extraction time, sonication amplitude, and sonication mode were investigated and optimized using a complete experimental design.
34146974	3	55	theme	polyphenols	513:523	arg1	UAE					505:507	UAE	505:507	UAE	505:507	Under this framework, the ultrasound-assisted extraction (UAE) of polyphenols from carob pulp was optimized by the maximization of the yield in total phenolics using response surface methodology.
34146974	3	55	theme	polyphenols	513:523	arg1	extraction					493:502	the ultrasound-assisted extraction	469:502	the ultrasound-assisted extraction (UAE) of polyphenols from carob pulp	469:539	Under this framework, the ultrasound-assisted extraction (UAE) of polyphenols from carob pulp was optimized by the maximization of the yield in total phenolics using response surface methodology.
34146974	1	56	theme	biological	250:259	arg1	effects					282:288	biological and health promoting effects	250:288	biological and health promoting effects	250:288	Polyphenols in carobs have recently attracted great attention due to their wide range of biological and health promoting effects.
34146974	3	57	theme	ultrasound-assisted	473:491	arg1	UAE					505:507	UAE	505:507	UAE	505:507	Under this framework, the ultrasound-assisted extraction (UAE) of polyphenols from carob pulp was optimized by the maximization of the yield in total phenolics using response surface methodology.
34146974	3	57	theme	ultrasound-assisted	473:491	arg1	extraction					493:502	the ultrasound-assisted extraction	469:502	the ultrasound-assisted extraction (UAE) of polyphenols from carob pulp	469:539	Under this framework, the ultrasound-assisted extraction (UAE) of polyphenols from carob pulp was optimized by the maximization of the yield in total phenolics using response surface methodology.
34146974	4	58	theme	extraction	717:726	arg1	time					728:731	extraction time	717:731	extraction time	717:731	In particular, the effects of solid-solvent ratio, solvent concentration, extraction time, sonication amplitude, and sonication mode were investigated and optimized using a complete experimental design.
34146974	2	59	theme	valuable	416:423	arg1	substances					435:444	these valuable bioactive substances	410:444	these valuable bioactive substances	410:444	A comprehensive study was conducted to find an optimum method for the extraction, purification and characterization of these valuable bioactive substances.
34146974	5	60	theme	conventional	863:874	arg1	techniques					887:896	conventional extraction techniques	863:896	conventional extraction techniques	863:896	In comparison to conventional extraction techniques, UAE offered a higher yield of antioxidants and a shorter processing time.
34146974	7	61	from	analysis	1105:1112	arg1	results					1088:1094	The results	1084:1094	The results from the analysis of real samples	1084:1128	The results from the analysis of real samples revealed the predominance of gallic acid and highlighted the great influence of the ripening stage on carobs composition.
34146974	5	62	theme	antioxidants	929:940	arg1	time					967:970	a shorter processing time	946:970	a shorter processing time	946:970	In comparison to conventional extraction techniques, UAE offered a higher yield of antioxidants and a shorter processing time.
34146974	5	62	theme	antioxidants	929:940	arg1	yield					920:924	a higher yield	911:924	a higher yield of antioxidants	911:940	In comparison to conventional extraction techniques, UAE offered a higher yield of antioxidants and a shorter processing time.
33188809	5	0	with	arabinogalactan	426:440	arg1	β-D-					447:450	β-D-	447:450	β-D-	447:450	It was identified by chemical and spectroscopic methods as arabinogalactan with β-D-(1→6)-galactan as backbone.
33188809	5	0	with	arabinogalactan	426:440	arg1	1→6					452:454	1→6	452:454	1→6	452:454	It was identified by chemical and spectroscopic methods as arabinogalactan with β-D-(1→6)-galactan as backbone.
33188809	4	1	theme	molecular	318:326	arg1	526.2 kDa					356:364	526.2 kDa	356:364	526.2 kDa	356:364	The molecular weight was determined to be 526.2 kDa.
33188809	4	1	theme	molecular	318:326	arg1	weight					328:333	The molecular weight	314:333	The molecular weight	314:333	The molecular weight was determined to be 526.2 kDa.
33188809	7	2	theme	α-L-Araf-	654:662	arg1	-α-L-Araf-					697:706	α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-	654:706	-α-L-Araf-	697:706	The side chains were identified to be α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-(1→ and α-D-Galp-(1→3)-α-L-Araf-(1→.
33188809	7	2	theme	α-L-Araf-	654:662	arg1	chains					625:630	The side chains	616:630	The side chains	616:630	The side chains were identified to be α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-(1→ and α-D-Galp-(1→3)-α-L-Araf-(1→.
33188809	1	3	contain	has	120:122	arg2	benefits					139:146	diverse health benefits	124:146	diverse health benefits	124:146	It is generally recognized that banana has diverse health benefits.
33188809	1	3	contain	has	120:122	arg1	banana					113:118	banana	113:118	banana	113:118	It is generally recognized that banana has diverse health benefits.
33188809	9	4	dep	drawn	832:836	arg1	below					841:845	below	841:845	below	841:845	The putative structure was drawn as below.
33188809	7	5	theme	1→	664:665	arg1	-α-L-Araf-					697:706	α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-	654:706	-α-L-Araf-	697:706	The side chains were identified to be α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-(1→ and α-D-Galp-(1→3)-α-L-Araf-(1→.
33188809	7	5	theme	1→	664:665	arg1	chains					625:630	The side chains	616:630	The side chains	616:630	The side chains were identified to be α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-(1→ and α-D-Galp-(1→3)-α-L-Araf-(1→.
33188809	9	6	theme	putative	809:816	arg1	structure					818:826	The putative structure	805:826	The putative structure	805:826	The putative structure was drawn as below.
33188809	7	7	dep	-α-L-Araf-	697:706	arg1	β-D-GlcpA-					668:677	α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-	654:706	β-D-GlcpA-	668:677	The side chains were identified to be α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-(1→ and α-D-Galp-(1→3)-α-L-Araf-(1→.
33188809	7	7	dep	-α-L-Araf-	697:706	arg1	α-L-Araf-					683:691	α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-	654:706	α-L-Araf-	683:691	The side chains were identified to be α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-(1→ and α-D-Galp-(1→3)-α-L-Araf-(1→.
33188809	7	7	dep	-α-L-Araf-	697:706	arg1	1→					740:741	1→	740:741	1→	740:741	The side chains were identified to be α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-(1→ and α-D-Galp-(1→3)-α-L-Araf-(1→.
33188809	7	7	dep	-α-L-Araf-	697:706	arg1	1→					708:709	1→	708:709	1→	708:709	The side chains were identified to be α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-(1→ and α-D-Galp-(1→3)-α-L-Araf-(1→.
33188809	11	8	theme	dose-dependent	941:954	arg1	behaviour					956:964	a dose-dependent behaviour	939:964	a dose-dependent behaviour	939:964	It could also inhibit ROS production with a dose-dependent behaviour.
33188809	8	9	theme	β-D-	764:767	arg1	-galactan					773:781	β-D-(1→6)-galactan	764:781	β-D-(1→6)-galactan at O-3	764:788	They were linked to β-D-(1→6)-galactan at O-3, respectively.
33188809	6	10	theme	molar	586:590	arg1	ratio					592:596	a relatively molar ratio	573:596	a relatively molar ratio of 5.8: 5.9: 1.0	573:613	This arabinogalactan was comprised of three monosaccharides, including Ara, Gal and GlcA with a relatively molar ratio of 5.8: 5.9: 1.0.
33188809	8	11	from	O-3	786:788	arg1	-galactan					773:781	β-D-(1→6)-galactan	764:781	β-D-(1→6)-galactan at O-3	764:788	They were linked to β-D-(1→6)-galactan at O-3, respectively.
33188809	8	12	theme	1→6	769:771	arg1	-galactan					773:781	β-D-(1→6)-galactan	764:781	β-D-(1→6)-galactan at O-3	764:788	They were linked to β-D-(1→6)-galactan at O-3, respectively.
33188809	2	13	theme	responsible	180:190	arg1	molecules					170:178	the molecules	166:178	the molecules responsible for the health benefits	166:214	However, most of the molecules responsible for the health benefits remain unknown.
33188809	3	14	theme	important	249:257	arg1	polysaccharide					259:272	an important polysaccharide	246:272	an important polysaccharide	246:272	In this work, an important polysaccharide was extracted from banana and purified.
33188809	0	15	theme	Chemical	27:34	arg1	characterization					36:51	Chemical characterization	27:51	Chemical characterization	27:51	Arabinogalactan in banana: Chemical characterization and pharmaceutical effects.
33188809	7	16	theme	1→	679:680	arg1	-α-L-Araf-					697:706	α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-	654:706	-α-L-Araf-	697:706	The side chains were identified to be α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-(1→ and α-D-Galp-(1→3)-α-L-Araf-(1→.
33188809	7	16	theme	1→	679:680	arg1	chains					625:630	The side chains	616:630	The side chains	616:630	The side chains were identified to be α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-(1→ and α-D-Galp-(1→3)-α-L-Araf-(1→.
33188809	0	17	theme	pharmaceutical	57:70	arg1	effects					72:78	pharmaceutical effects	57:78	pharmaceutical effects	57:78	Arabinogalactan in banana: Chemical characterization and pharmaceutical effects.
33188809	10	18	theme	NO	882:883	arg1	production					885:894	NO production	882:894	NO production	882:894	This arabinogalactan could induce NO production.
33188809	0	19	from	Arabinogalactan	0:14	arg1	banana					19:24	banana	19:24	banana	19:24	Arabinogalactan in banana: Chemical characterization and pharmaceutical effects.
33188809	7	20	theme	1→5	693:695	arg1	-α-L-Araf-					697:706	α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-	654:706	-α-L-Araf-	697:706	The side chains were identified to be α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-(1→ and α-D-Galp-(1→3)-α-L-Araf-(1→.
33188809	7	20	theme	1→5	693:695	arg1	chains					625:630	The side chains	616:630	The side chains	616:630	The side chains were identified to be α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-(1→ and α-D-Galp-(1→3)-α-L-Araf-(1→.
33188809	1	21	theme	diverse	124:130	arg1	benefits					139:146	diverse health benefits	124:146	diverse health benefits	124:146	It is generally recognized that banana has diverse health benefits.
33188809	2	22	theme	health	200:205	arg1	benefits					207:214	the health benefits	196:214	the health benefits	196:214	However, most of the molecules responsible for the health benefits remain unknown.
33188809	1	23	theme	health	132:137	arg1	benefits					139:146	diverse health benefits	124:146	diverse health benefits	124:146	It is generally recognized that banana has diverse health benefits.
33188809	7	24	theme	side	620:623	arg1	-α-L-Araf-					697:706	α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-	654:706	-α-L-Araf-	697:706	The side chains were identified to be α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-(1→ and α-D-Galp-(1→3)-α-L-Araf-(1→.
33188809	7	24	theme	side	620:623	arg1	chains					625:630	The side chains	616:630	The side chains	616:630	The side chains were identified to be α-L-Araf-(1→, β-D-GlcpA-(1→, α-L-Araf-(1→5)-α-L-Araf-(1→ and α-D-Galp-(1→3)-α-L-Araf-(1→.
33188809	6	25	theme	1.0	611:613	arg1	ratio					592:596	a relatively molar ratio	573:596	a relatively molar ratio of 5.8: 5.9: 1.0	573:613	This arabinogalactan was comprised of three monosaccharides, including Ara, Gal and GlcA with a relatively molar ratio of 5.8: 5.9: 1.0.
33188809	5	26	theme	chemical	388:395	arg1	methods					415:421	chemical and spectroscopic methods	388:421	chemical and spectroscopic methods	388:421	It was identified by chemical and spectroscopic methods as arabinogalactan with β-D-(1→6)-galactan as backbone.
33188809	8	27	attach	linked	754:759	arg2	They					744:747	They	744:747	They	744:747	They were linked to β-D-(1→6)-galactan at O-3, respectively.
33188809	8	27	attach	linked	754:759	arg1	-galactan					773:781	β-D-(1→6)-galactan	764:781	β-D-(1→6)-galactan at O-3	764:788	They were linked to β-D-(1→6)-galactan at O-3, respectively.
33188809	0	28	dep	Arabinogalactan	0:14	arg1	characterization					36:51	Chemical characterization	27:51	Chemical characterization	27:51	Arabinogalactan in banana: Chemical characterization and pharmaceutical effects.
33188809	0	28	dep	Arabinogalactan	0:14	arg1	effects					72:78	pharmaceutical effects	57:78	pharmaceutical effects	57:78	Arabinogalactan in banana: Chemical characterization and pharmaceutical effects.
33188809	11	29	theme	ROS	919:921	arg1	production					923:932	ROS production	919:932	ROS production	919:932	It could also inhibit ROS production with a dose-dependent behaviour.
33188809	5	30	theme	spectroscopic	401:413	arg1	methods					415:421	chemical and spectroscopic methods	388:421	chemical and spectroscopic methods	388:421	It was identified by chemical and spectroscopic methods as arabinogalactan with β-D-(1→6)-galactan as backbone.
34810234	7	0	theme	IBSH	1110:1113	arg1	microbiomes					1115:1125	IBSH microbiomes	1110:1125	IBSH microbiomes	1110:1125	IBSH microbiomes were similar to controls.
34810234	5	1	dep	RESULTS	789:795	arg1	analysis					810:817	Unsupervised analysis	797:817	RESULTS Unsupervised analysis of baseline IBS cases pre-diet	789:848	RESULTS Unsupervised analysis of baseline IBS cases pre-diet identified two distinct microbiota profiles, which we refer to as IBSP (pathogenic-like) and IBSH (health-like) subtypes.
34810234	3	2	theme	high-resolution	541:555	arg1	profiles					582:589	high-resolution taxonomic and functional profiles	541:589	high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs)	541:664	DESIGN We used metagenomics to determine high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs) on their usual diet.
34810234	8	3	theme	FODMAP	1164:1169	arg1	diet					1171:1174	the low FODMAP diet	1156:1174	the low FODMAP diet	1156:1174	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	5	4	theme	microbiota	874:883	arg1	profiles					885:892	two distinct microbiota profiles	861:892	two distinct microbiota profiles	861:892	RESULTS Unsupervised analysis of baseline IBS cases pre-diet identified two distinct microbiota profiles, which we refer to as IBSP (pathogenic-like) and IBSH (health-like) subtypes.
34810234	12	5	from	IBSP	1796:1799	arg1	effectiveness					1759:1771	The effectiveness	1755:1771	The effectiveness of FODMAP reduction in IBSP	1755:1799	The effectiveness of FODMAP reduction in IBSP may result from the alterations in gut microbiota and metabolites produced.
34810234	8	6	theme	IBSP	1230:1233	arg1	signature					1235:1243	the IBSP signature	1226:1243	the IBSP signature	1226:1243	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	10	7	theme	CONCLUSION	1529:1538	arg1	%					1542:1542	CONCLUSION 50%	1529:1542	CONCLUSION 50% of IBS cases	1529:1555	CONCLUSION 50% of IBS cases manifested a 'pathogenic' gut microbial signature.
34810234	10	7	theme	CONCLUSION	1529:1538	arg1	cases					1551:1555	IBS cases	1547:1555	IBS cases	1547:1555	CONCLUSION 50% of IBS cases manifested a 'pathogenic' gut microbial signature.
34810234	11	8	theme	enhanced	1698:1705	arg1	responsiveness					1716:1729	an enhanced clinical responsiveness	1695:1729	an enhanced clinical responsiveness to the dietary therapy	1695:1752	This shifted towards the healthy profile on the low FODMAP diet; and IBSP cases showed an enhanced clinical responsiveness to the dietary therapy.
34810234	5	9	theme	IBS	831:833	arg1	cases					835:839	baseline IBS cases	822:839	baseline IBS cases pre-diet	822:848	RESULTS Unsupervised analysis of baseline IBS cases pre-diet identified two distinct microbiota profiles, which we refer to as IBSP (pathogenic-like) and IBSH (health-like) subtypes.
34810234	1	10	theme	OBJECTIVE	111:119	arg1	FODMAPs					130:136	OBJECTIVE Reducing FODMAPs	111:136	OBJECTIVE Reducing FODMAPs (fermentable oligosaccharides, disaccharides, monosaccharides and polyols)	111:211	OBJECTIVE Reducing FODMAPs (fermentable oligosaccharides, disaccharides, monosaccharides and polyols) can be clinically beneficial in IBS but the mechanism is incompletely understood.
34810234	8	11	theme	primary	1401:1407	arg1	genes					1419:1423	primary metabolic genes	1401:1423	primary metabolic genes	1401:1423	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	3	12	theme	IBS	620:622	arg1	cases					624:628	IBS cases	620:628	IBS cases	620:628	DESIGN We used metagenomics to determine high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs) on their usual diet.
34810234	5	13	theme	pre-diet	841:848	arg1	cases					835:839	baseline IBS cases	822:839	baseline IBS cases pre-diet	822:848	RESULTS Unsupervised analysis of baseline IBS cases pre-diet identified two distinct microbiota profiles, which we refer to as IBSP (pathogenic-like) and IBSH (health-like) subtypes.
34810234	8	14	theme	genes	1419:1423	arg1	normalisation					1384:1396	normalisation	1384:1396	normalisation of primary metabolic genes	1384:1423	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	8	14	theme	genes	1419:1423	arg1	increase					1300:1307	an increase	1297:1307	an increase in Bacteroidetes (p=0.009)	1297:1334	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	8	14	theme	genes	1419:1423	arg1	decrease					1339:1346	a decrease	1337:1346	a decrease in Firmicutes species (p=0.004)	1337:1378	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	10	15	theme	IBS	1547:1549	arg1	cases					1551:1555	IBS cases	1547:1555	IBS cases	1547:1555	CONCLUSION 50% of IBS cases manifested a 'pathogenic' gut microbial signature.
34810234	1	16	from	IBS	245:247	arg1	beneficial					231:240	beneficial	231:240	beneficial	231:240	OBJECTIVE Reducing FODMAPs (fermentable oligosaccharides, disaccharides, monosaccharides and polyols) can be clinically beneficial in IBS but the mechanism is incompletely understood.
34810234	3	17	theme	microbiota	604:613	arg1	profiles					582:589	high-resolution taxonomic and functional profiles	541:589	high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs)	541:664	DESIGN We used metagenomics to determine high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs) on their usual diet.
34810234	9	18	theme	low	1455:1457	arg1	diet					1466:1469	the low FODMAP diet	1451:1469	the low FODMAP diet	1451:1469	The clinical response to the low FODMAP diet was greater in IBSP subjects compared with IBSH (p=0.02).
34810234	1	19	from	beneficial	231:240	arg1	IBS					245:247	IBS	245:247	IBS	245:247	OBJECTIVE Reducing FODMAPs (fermentable oligosaccharides, disaccharides, monosaccharides and polyols) can be clinically beneficial in IBS but the mechanism is incompletely understood.
34810234	13	20	theme	IBS	2038:2040	arg1	mechanisms					2053:2062	IBS pathogenic mechanisms	2038:2062	IBS pathogenic mechanisms	2038:2062	Microbiota signatures could be useful as biomarkers to guide IBS treatment; and investigating IBSP species and metabolic pathways might yield insights regarding IBS pathogenic mechanisms.
34810234	1	21	theme	Reducing	121:128	arg1	FODMAPs					130:136	OBJECTIVE Reducing FODMAPs	111:136	OBJECTIVE Reducing FODMAPs (fermentable oligosaccharides, disaccharides, monosaccharides and polyols)	111:211	OBJECTIVE Reducing FODMAPs (fermentable oligosaccharides, disaccharides, monosaccharides and polyols) can be clinically beneficial in IBS but the mechanism is incompletely understood.
34810234	11	22	from	profile	1641:1647	arg1	diet					1667:1670	the low FODMAP diet	1652:1670	the low FODMAP diet	1652:1670	This shifted towards the healthy profile on the low FODMAP diet; and IBSP cases showed an enhanced clinical responsiveness to the dietary therapy.
34810234	4	23	theme	Clinical	687:694	arg1	response					696:703	Clinical response	687:703	Clinical response	687:703	Clinical response and microbiota changes were studied in 41 pairs after 4 weeks on a low FODMAP diet.
34810234	11	24	theme	FODMAP	1660:1665	arg1	diet					1667:1670	the low FODMAP diet	1652:1670	the low FODMAP diet	1652:1670	This shifted towards the healthy profile on the low FODMAP diet; and IBSP cases showed an enhanced clinical responsiveness to the dietary therapy.
34810234	13	25	theme	metabolic	1988:1996	arg1	pathways					1998:2005	metabolic pathways	1988:2005	metabolic pathways	1988:2005	Microbiota signatures could be useful as biomarkers to guide IBS treatment; and investigating IBSP species and metabolic pathways might yield insights regarding IBS pathogenic mechanisms.
34810234	1	26	theme	fermentable	139:149	arg1	oligosaccharides					151:166	fermentable oligosaccharides	139:166	fermentable oligosaccharides	139:166	OBJECTIVE Reducing FODMAPs (fermentable oligosaccharides, disaccharides, monosaccharides and polyols) can be clinically beneficial in IBS but the mechanism is incompletely understood.
34810234	2	27	theme	action	492:497	arg1	mode					484:487	its mode	480:487	its mode of action	480:497	We aimed to detect microbial signatures that might predict response to the low FODMAP diet and assess whether microbiota compositional and functional shifts could provide insights into its mode of action.
34810234	9	28	theme	IBSP	1486:1489	arg1	subjects					1491:1498	IBSP subjects	1486:1498	IBSP subjects	1486:1498	The clinical response to the low FODMAP diet was greater in IBSP subjects compared with IBSH (p=0.02).
34810234	6	29	theme	IBSP	972:975	arg1	microbiomes					977:987	IBSP microbiomes	972:987	IBSP microbiomes	972:987	IBSP microbiomes were enriched in Firmicutes and genes for amino acid and carbohydrate metabolism, but depleted in Bacteroidetes species.
34810234	11	30	theme	IBSP	1677:1680	arg1	cases					1682:1686	IBSP cases	1677:1686	IBSP cases	1677:1686	This shifted towards the healthy profile on the low FODMAP diet; and IBSP cases showed an enhanced clinical responsiveness to the dietary therapy.
34810234	13	31	theme	investigating	1957:1969	arg1	species					1976:1982	investigating IBSP species	1957:1982	investigating IBSP species	1957:1982	Microbiota signatures could be useful as biomarkers to guide IBS treatment; and investigating IBSP species and metabolic pathways might yield insights regarding IBS pathogenic mechanisms.
34810234	0	32	theme	FODMAP	98:103	arg1	diet					105:108	the low FODMAP diet	90:108	the low FODMAP diet	90:108	Two microbiota subtypes identified in irritable bowel syndrome with distinct responses to the low FODMAP diet.
34810234	3	33	dep	DESIGN	500:505	arg1	used					510:513	used	510:513	used metagenomics to determine high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs) on their usual diet	510:684	DESIGN We used metagenomics to determine high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs) on their usual diet.
34810234	6	34	theme	carbohydrate	1046:1057	arg1	metabolism					1059:1068	carbohydrate metabolism	1046:1068	carbohydrate metabolism	1046:1068	IBSP microbiomes were enriched in Firmicutes and genes for amino acid and carbohydrate metabolism, but depleted in Bacteroidetes species.
34810234	3	35	from	controls	644:651	arg1	profiles					582:589	high-resolution taxonomic and functional profiles	541:589	high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs)	541:664	DESIGN We used metagenomics to determine high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs) on their usual diet.
34810234	5	36	theme	Unsupervised	797:808	arg1	analysis					810:817	Unsupervised analysis	797:817	RESULTS Unsupervised analysis of baseline IBS cases pre-diet	789:848	RESULTS Unsupervised analysis of baseline IBS cases pre-diet identified two distinct microbiota profiles, which we refer to as IBSP (pathogenic-like) and IBSH (health-like) subtypes.
34810234	5	37	theme	pathogenic-like	922:936	arg1	IBSP					916:919	IBSP (pathogenic-like) and IBSH (health-like) subtypes	916:969	IBSP	916:919	RESULTS Unsupervised analysis of baseline IBS cases pre-diet identified two distinct microbiota profiles, which we refer to as IBSP (pathogenic-like) and IBSH (health-like) subtypes.
34810234	12	38	theme	reduction	1783:1791	arg1	effectiveness					1759:1771	The effectiveness	1755:1771	The effectiveness of FODMAP reduction in IBSP	1755:1799	The effectiveness of FODMAP reduction in IBSP may result from the alterations in gut microbiota and metabolites produced.
34810234	11	39	theme	healthy	1633:1639	arg1	profile					1641:1647	the healthy profile	1629:1647	the healthy profile on the low FODMAP diet	1629:1670	This shifted towards the healthy profile on the low FODMAP diet; and IBSP cases showed an enhanced clinical responsiveness to the dietary therapy.
34810234	2	40	theme	microbiota	405:414	arg1	shifts					445:450	microbiota compositional and functional shifts	405:450	microbiota compositional and functional shifts	405:450	We aimed to detect microbial signatures that might predict response to the low FODMAP diet and assess whether microbiota compositional and functional shifts could provide insights into its mode of action.
34810234	3	41	theme	n=56	654:657	arg1	pairs					659:663	n=56 pairs	654:663	n=56 pairs	654:663	DESIGN We used metagenomics to determine high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs) on their usual diet.
34810234	3	41	theme	n=56	654:657	arg1	controls					644:651	household controls	634:651	household controls (n=56 pairs)	634:664	DESIGN We used metagenomics to determine high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs) on their usual diet.
34810234	5	42	dep	identified	850:859	arg1	refer					904:908	refer	904:908	refer to as IBSP (pathogenic-like) and IBSH (health-like) subtypes	904:969	RESULTS Unsupervised analysis of baseline IBS cases pre-diet identified two distinct microbiota profiles, which we refer to as IBSP (pathogenic-like) and IBSH (health-like) subtypes.
34810234	0	43	theme	bowel	48:52	arg1	syndrome					54:61	irritable bowel syndrome	38:61	irritable bowel syndrome with distinct responses to the low FODMAP diet	38:108	Two microbiota subtypes identified in irritable bowel syndrome with distinct responses to the low FODMAP diet.
34810234	11	44	theme	low	1656:1658	arg1	diet					1667:1670	the low FODMAP diet	1652:1670	the low FODMAP diet	1652:1670	This shifted towards the healthy profile on the low FODMAP diet; and IBSP cases showed an enhanced clinical responsiveness to the dietary therapy.
34810234	3	45	used	used	510:513	arg2	We					507:508	We	507:508	We	507:508	DESIGN We used metagenomics to determine high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs) on their usual diet.
34810234	6	46	theme	Bacteroidetes	1087:1099	arg1	species					1101:1107	Bacteroidetes species	1087:1107	Bacteroidetes species	1087:1107	IBSP microbiomes were enriched in Firmicutes and genes for amino acid and carbohydrate metabolism, but depleted in Bacteroidetes species.
34810234	2	47	theme	low	370:372	arg1	diet					381:384	the low FODMAP diet	366:384	the low FODMAP diet	366:384	We aimed to detect microbial signatures that might predict response to the low FODMAP diet and assess whether microbiota compositional and functional shifts could provide insights into its mode of action.
34810234	13	48	theme	guide	1932:1936	arg1	treatment					1942:1950	guide IBS treatment	1932:1950	guide IBS treatment	1932:1950	Microbiota signatures could be useful as biomarkers to guide IBS treatment; and investigating IBSP species and metabolic pathways might yield insights regarding IBS pathogenic mechanisms.
34810234	8	49	from	decrease	1339:1346	arg1	p=0.009					1327:1333	p=0.009	1327:1333	p=0.009	1327:1333	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	8	49	from	decrease	1339:1346	arg1	Bacteroidetes					1312:1324	Bacteroidetes	1312:1324	Bacteroidetes (p=0.009)	1312:1334	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	8	49	from	decrease	1339:1346	arg1	p=0.004					1371:1377	p=0.004	1371:1377	p=0.004	1371:1377	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	8	49	from	decrease	1339:1346	arg1	species					1362:1368	Firmicutes species	1351:1368	Firmicutes species (p=0.004)	1351:1378	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	3	50	theme	taxonomic	557:565	arg1	profiles					582:589	high-resolution taxonomic and functional profiles	541:589	high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs)	541:664	DESIGN We used metagenomics to determine high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs) on their usual diet.
34810234	2	51	theme	microbial	314:322	arg1	signatures					324:333	microbial signatures	314:333	microbial signatures that might predict response to the low FODMAP diet and assess whether microbiota compositional and functional shifts could provide insights into its mode of action	314:497	We aimed to detect microbial signatures that might predict response to the low FODMAP diet and assess whether microbiota compositional and functional shifts could provide insights into its mode of action.
34810234	5	52	theme	distinct	865:872	arg1	profiles					885:892	two distinct microbiota profiles	861:892	two distinct microbiota profiles	861:892	RESULTS Unsupervised analysis of baseline IBS cases pre-diet identified two distinct microbiota profiles, which we refer to as IBSP (pathogenic-like) and IBSH (health-like) subtypes.
34810234	8	53	theme	low	1160:1162	arg1	diet					1171:1174	the low FODMAP diet	1156:1174	the low FODMAP diet	1156:1174	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	13	54	theme	Microbiota	1877:1886	arg1	signatures					1888:1897	Microbiota signatures	1877:1897	Microbiota signatures	1877:1897	Microbiota signatures could be useful as biomarkers to guide IBS treatment; and investigating IBSP species and metabolic pathways might yield insights regarding IBS pathogenic mechanisms.
34810234	3	55	theme	functional	571:580	arg1	profiles					582:589	high-resolution taxonomic and functional profiles	541:589	high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs)	541:664	DESIGN We used metagenomics to determine high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs) on their usual diet.
34810234	8	56	from	normalisation	1384:1396	arg1	p=0.009					1327:1333	p=0.009	1327:1333	p=0.009	1327:1333	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	8	56	from	normalisation	1384:1396	arg1	Bacteroidetes					1312:1324	Bacteroidetes	1312:1324	Bacteroidetes (p=0.009)	1312:1334	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	8	56	from	normalisation	1384:1396	arg1	p=0.004					1371:1377	p=0.004	1371:1377	p=0.004	1371:1377	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	8	56	from	normalisation	1384:1396	arg1	species					1362:1368	Firmicutes species	1351:1368	Firmicutes species (p=0.004)	1351:1378	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	5	57	theme	baseline	822:829	arg1	cases					835:839	baseline IBS cases	822:839	baseline IBS cases pre-diet	822:848	RESULTS Unsupervised analysis of baseline IBS cases pre-diet identified two distinct microbiota profiles, which we refer to as IBSP (pathogenic-like) and IBSH (health-like) subtypes.
34810234	8	58	theme	metabolic	1409:1417	arg1	genes					1419:1423	primary metabolic genes	1401:1423	primary metabolic genes	1401:1423	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	11	59	theme	clinical	1707:1714	arg1	responsiveness					1716:1729	an enhanced clinical responsiveness	1695:1729	an enhanced clinical responsiveness to the dietary therapy	1695:1752	This shifted towards the healthy profile on the low FODMAP diet; and IBSP cases showed an enhanced clinical responsiveness to the dietary therapy.
34810234	5	60	theme	cases	835:839	arg1	analysis					810:817	Unsupervised analysis	797:817	RESULTS Unsupervised analysis of baseline IBS cases pre-diet	789:848	RESULTS Unsupervised analysis of baseline IBS cases pre-diet identified two distinct microbiota profiles, which we refer to as IBSP (pathogenic-like) and IBSH (health-like) subtypes.
34810234	10	61	theme	cases	1551:1555	arg1	%					1542:1542	CONCLUSION 50%	1529:1542	CONCLUSION 50% of IBS cases	1529:1555	CONCLUSION 50% of IBS cases manifested a 'pathogenic' gut microbial signature.
34810234	10	61	theme	cases	1551:1555	arg1	cases					1551:1555	IBS cases	1547:1555	IBS cases	1547:1555	CONCLUSION 50% of IBS cases manifested a 'pathogenic' gut microbial signature.
34810234	3	62	theme	stool	598:602	arg1	microbiota					604:613	the stool microbiota	594:613	the stool microbiota	594:613	DESIGN We used metagenomics to determine high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs) on their usual diet.
34810234	8	63	theme	control	1186:1192	arg1	microbiota					1194:1203	control microbiota	1186:1203	control microbiota	1186:1203	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	12	64	from	effectiveness	1759:1771	arg1	IBSP					1796:1799	IBSP	1796:1799	IBSP	1796:1799	The effectiveness of FODMAP reduction in IBSP may result from the alterations in gut microbiota and metabolites produced.
34810234	4	65	theme	microbiota	709:718	arg1	changes					720:726	microbiota changes	709:726	microbiota changes	709:726	Clinical response and microbiota changes were studied in 41 pairs after 4 weeks on a low FODMAP diet.
34810234	11	66	theme	dietary	1738:1744	arg1	therapy					1746:1752	the dietary therapy	1734:1752	the dietary therapy	1734:1752	This shifted towards the healthy profile on the low FODMAP diet; and IBSP cases showed an enhanced clinical responsiveness to the dietary therapy.
34810234	4	67	theme	FODMAP	776:781	arg1	diet					783:786	a low FODMAP diet	770:786	a low FODMAP diet	770:786	Clinical response and microbiota changes were studied in 41 pairs after 4 weeks on a low FODMAP diet.
34810234	3	68	theme	household	634:642	arg1	pairs					659:663	n=56 pairs	654:663	n=56 pairs	654:663	DESIGN We used metagenomics to determine high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs) on their usual diet.
34810234	3	68	theme	household	634:642	arg1	controls					644:651	household controls	634:651	household controls (n=56 pairs)	634:664	DESIGN We used metagenomics to determine high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs) on their usual diet.
34810234	9	69	theme	FODMAP	1459:1464	arg1	diet					1466:1469	the low FODMAP diet	1451:1469	the low FODMAP diet	1451:1469	The clinical response to the low FODMAP diet was greater in IBSP subjects compared with IBSH (p=0.02).
34810234	8	70	theme	health-associated	1263:1279	arg1	microbiome					1281:1290	a health-associated microbiome	1261:1290	a health-associated microbiome	1261:1290	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	0	71	theme	distinct	68:75	arg1	responses					77:85	distinct responses	68:85	distinct responses to the low FODMAP diet	68:108	Two microbiota subtypes identified in irritable bowel syndrome with distinct responses to the low FODMAP diet.
34810234	13	72	theme	pathogenic	2042:2051	arg1	mechanisms					2053:2062	IBS pathogenic mechanisms	2038:2062	IBS pathogenic mechanisms	2038:2062	Microbiota signatures could be useful as biomarkers to guide IBS treatment; and investigating IBSP species and metabolic pathways might yield insights regarding IBS pathogenic mechanisms.
34810234	12	73	theme	gut	1836:1838	arg1	microbiota					1840:1849	gut microbiota	1836:1849	gut microbiota	1836:1849	The effectiveness of FODMAP reduction in IBSP may result from the alterations in gut microbiota and metabolites produced.
34810234	12	74	from	alterations	1821:1831	arg1	metabolites					1855:1865	metabolites	1855:1865	metabolites	1855:1865	The effectiveness of FODMAP reduction in IBSP may result from the alterations in gut microbiota and metabolites produced.
34810234	12	74	from	alterations	1821:1831	arg1	microbiota					1840:1849	gut microbiota	1836:1849	gut microbiota	1836:1849	The effectiveness of FODMAP reduction in IBSP may result from the alterations in gut microbiota and metabolites produced.
34810234	0	75	theme	low	94:96	arg1	diet					105:108	the low FODMAP diet	90:108	the low FODMAP diet	90:108	Two microbiota subtypes identified in irritable bowel syndrome with distinct responses to the low FODMAP diet.
34810234	5	76	theme	IBSH	943:946	arg1	subtypes					962:969	IBSP (pathogenic-like) and IBSH (health-like) subtypes	916:969	subtypes	962:969	RESULTS Unsupervised analysis of baseline IBS cases pre-diet identified two distinct microbiota profiles, which we refer to as IBSP (pathogenic-like) and IBSH (health-like) subtypes.
34810234	1	77	dep	FODMAPs	130:136	arg1	monosaccharides					184:198	monosaccharides	184:198	monosaccharides	184:198	OBJECTIVE Reducing FODMAPs (fermentable oligosaccharides, disaccharides, monosaccharides and polyols) can be clinically beneficial in IBS but the mechanism is incompletely understood.
34810234	1	77	dep	FODMAPs	130:136	arg1	disaccharides					169:181	disaccharides	169:181	disaccharides	169:181	OBJECTIVE Reducing FODMAPs (fermentable oligosaccharides, disaccharides, monosaccharides and polyols) can be clinically beneficial in IBS but the mechanism is incompletely understood.
34810234	1	77	dep	FODMAPs	130:136	arg1	oligosaccharides					151:166	fermentable oligosaccharides	139:166	fermentable oligosaccharides	139:166	OBJECTIVE Reducing FODMAPs (fermentable oligosaccharides, disaccharides, monosaccharides and polyols) can be clinically beneficial in IBS but the mechanism is incompletely understood.
34810234	1	77	dep	FODMAPs	130:136	arg1	polyols					204:210	polyols	204:210	polyols	204:210	OBJECTIVE Reducing FODMAPs (fermentable oligosaccharides, disaccharides, monosaccharides and polyols) can be clinically beneficial in IBS but the mechanism is incompletely understood.
34810234	3	78	from	cases	624:628	arg1	profiles					582:589	high-resolution taxonomic and functional profiles	541:589	high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs)	541:664	DESIGN We used metagenomics to determine high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs) on their usual diet.
34810234	13	79	theme	IBSP	1971:1974	arg1	species					1976:1982	investigating IBSP species	1957:1982	investigating IBSP species	1957:1982	Microbiota signatures could be useful as biomarkers to guide IBS treatment; and investigating IBSP species and metabolic pathways might yield insights regarding IBS pathogenic mechanisms.
34810234	9	80	theme	clinical	1430:1437	arg1	greater					1475:1481	greater	1475:1481	greater	1475:1481	The clinical response to the low FODMAP diet was greater in IBSP subjects compared with IBSH (p=0.02).
34810234	9	80	theme	clinical	1430:1437	arg1	response					1439:1446	The clinical response	1426:1446	The clinical response to the low FODMAP diet	1426:1469	The clinical response to the low FODMAP diet was greater in IBSP subjects compared with IBSH (p=0.02).
34810234	5	81	theme	health-like	949:959	arg1	subtypes					962:969	IBSP (pathogenic-like) and IBSH (health-like) subtypes	916:969	subtypes	962:969	RESULTS Unsupervised analysis of baseline IBS cases pre-diet identified two distinct microbiota profiles, which we refer to as IBSP (pathogenic-like) and IBSH (health-like) subtypes.
34810234	0	82	theme	microbiota	4:13	arg1	subtypes					15:22	Two microbiota subtypes	0:22	Two microbiota subtypes	0:22	Two microbiota subtypes identified in irritable bowel syndrome with distinct responses to the low FODMAP diet.
34810234	12	83	from	reduction	1783:1791	arg1	IBSP					1796:1799	IBSP	1796:1799	IBSP	1796:1799	The effectiveness of FODMAP reduction in IBSP may result from the alterations in gut microbiota and metabolites produced.
34810234	8	84	from	increase	1300:1307	arg1	p=0.009					1327:1333	p=0.009	1327:1333	p=0.009	1327:1333	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	8	84	from	increase	1300:1307	arg1	Bacteroidetes					1312:1324	Bacteroidetes	1312:1324	Bacteroidetes (p=0.009)	1312:1334	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	8	84	from	increase	1300:1307	arg1	p=0.004					1371:1377	p=0.004	1371:1377	p=0.004	1371:1377	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	8	84	from	increase	1300:1307	arg1	species					1362:1368	Firmicutes species	1351:1368	Firmicutes species (p=0.004)	1351:1378	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	2	85	theme	FODMAP	374:379	arg1	diet					381:384	the low FODMAP diet	366:384	the low FODMAP diet	366:384	We aimed to detect microbial signatures that might predict response to the low FODMAP diet and assess whether microbiota compositional and functional shifts could provide insights into its mode of action.
34810234	6	86	theme	amino	1031:1035	arg1	acid					1037:1040	amino acid	1031:1040	amino acid	1031:1040	IBSP microbiomes were enriched in Firmicutes and genes for amino acid and carbohydrate metabolism, but depleted in Bacteroidetes species.
34810234	2	87	theme	functional	434:443	arg1	shifts					445:450	microbiota compositional and functional shifts	405:450	microbiota compositional and functional shifts	405:450	We aimed to detect microbial signatures that might predict response to the low FODMAP diet and assess whether microbiota compositional and functional shifts could provide insights into its mode of action.
34810234	2	88	theme	compositional	416:428	arg1	shifts					445:450	microbiota compositional and functional shifts	405:450	microbiota compositional and functional shifts	405:450	We aimed to detect microbial signatures that might predict response to the low FODMAP diet and assess whether microbiota compositional and functional shifts could provide insights into its mode of action.
34810234	0	89	theme	irritable	38:46	arg1	syndrome					54:61	irritable bowel syndrome	38:61	irritable bowel syndrome with distinct responses to the low FODMAP diet	38:108	Two microbiota subtypes identified in irritable bowel syndrome with distinct responses to the low FODMAP diet.
34810234	8	90	theme	Firmicutes	1351:1360	arg1	p=0.004					1371:1377	p=0.004	1371:1377	p=0.004	1371:1377	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	8	90	theme	Firmicutes	1351:1360	arg1	species					1362:1368	Firmicutes species	1351:1368	Firmicutes species (p=0.004)	1351:1378	On the low FODMAP diet, IBSH and control microbiota were unaffected, but the IBSP signature shifted towards a health-associated microbiome with an increase in Bacteroidetes (p=0.009), a decrease in Firmicutes species (p=0.004) and normalisation of primary metabolic genes.
34810234	13	91	theme	IBS	1938:1940	arg1	treatment					1942:1950	guide IBS treatment	1932:1950	guide IBS treatment	1932:1950	Microbiota signatures could be useful as biomarkers to guide IBS treatment; and investigating IBSP species and metabolic pathways might yield insights regarding IBS pathogenic mechanisms.
34810234	10	92	theme	microbial	1587:1595	arg1	signature					1597:1605	a 'pathogenic' gut microbial signature	1568:1605	a 'pathogenic' gut microbial signature	1568:1605	CONCLUSION 50% of IBS cases manifested a 'pathogenic' gut microbial signature.
34810234	3	93	theme	usual	675:679	arg1	diet					681:684	their usual diet	669:684	their usual diet	669:684	DESIGN We used metagenomics to determine high-resolution taxonomic and functional profiles of the stool microbiota from IBS cases and household controls (n=56 pairs) on their usual diet.
34810234	12	94	theme	FODMAP	1776:1781	arg1	reduction					1783:1791	FODMAP reduction	1776:1791	FODMAP reduction in IBSP	1776:1799	The effectiveness of FODMAP reduction in IBSP may result from the alterations in gut microbiota and metabolites produced.
34810234	4	95	theme	low	772:774	arg1	diet					783:786	a low FODMAP diet	770:786	a low FODMAP diet	770:786	Clinical response and microbiota changes were studied in 41 pairs after 4 weeks on a low FODMAP diet.
34810234	0	96	with	syndrome	54:61	arg1	responses					77:85	distinct responses	68:85	distinct responses to the low FODMAP diet	68:108	Two microbiota subtypes identified in irritable bowel syndrome with distinct responses to the low FODMAP diet.
34419862	2	0	theme	high-capacity	497:509	arg1	sorption					527:534	high-capacity uraniumVI (UVI) sorption	497:534	high-capacity uraniumVI (UVI) sorption	497:534	β-Cyclodextrin was intercalated into the mixed-valent iron-based hydroxide layers to synthesize new green rust materials (β-CD GRs), pursuing high-capacity uraniumVI (UVI) sorption.
34419862	1	1	theme	oxide	338:342	arg1	materials					344:352	FeII/FeIII (oxyhydr)oxide materials	318:352	FeII/FeIII (oxyhydr)oxide materials	318:352	The influence of intercalated anions on the structure and composition of green rusts supplies a theoretical possibility for the investigation of the structural modification of FeII/FeIII (oxyhydr)oxide materials.
34419862	11	2	theme	several	1607:1613	arg1	pathways					1624:1631	several reaction pathways	1607:1631	several reaction pathways	1607:1631	The intercalation of β-CD brought obvious enhancement of UVI species sorption to the GR material, which was combinedly driven by several reaction pathways and different from the unmodified GRs.
34419862	2	3	theme	hydroxide	420:428	arg1	layers					430:435	the mixed-valent iron-based hydroxide layers	392:435	the mixed-valent iron-based hydroxide layers	392:435	β-Cyclodextrin was intercalated into the mixed-valent iron-based hydroxide layers to synthesize new green rust materials (β-CD GRs), pursuing high-capacity uraniumVI (UVI) sorption.
34419862	1	4	theme	materials	344:352	arg1	modification					302:313	the structural modification	287:313	the structural modification of FeII/FeIII (oxyhydr)oxide materials	287:352	The influence of intercalated anions on the structure and composition of green rusts supplies a theoretical possibility for the investigation of the structural modification of FeII/FeIII (oxyhydr)oxide materials.
34419862	6	5	theme	Langmuir	947:954	arg1	model					965:969	Langmuir isotherm model	947:969	Langmuir isotherm model	947:969	The pseudo-first-order kinetic model and Langmuir isotherm model were appropriate in fitting the whole adsorption process.
34419862	11	6	theme	sorption	1547:1554	arg1	enhancement					1520:1530	obvious enhancement	1512:1530	obvious enhancement of UVI species sorption to the GR material, which was combinedly driven by several reaction pathways and different from the unmodified GRs	1512:1669	The intercalation of β-CD brought obvious enhancement of UVI species sorption to the GR material, which was combinedly driven by several reaction pathways and different from the unmodified GRs.
34419862	1	7	theme	intercalated	159:170	arg1	anions					172:177	intercalated anions	159:177	intercalated anions	159:177	The influence of intercalated anions on the structure and composition of green rusts supplies a theoretical possibility for the investigation of the structural modification of FeII/FeIII (oxyhydr)oxide materials.
34419862	2	8	theme	UVI	522:524	arg1	sorption					527:534	high-capacity uraniumVI (UVI) sorption	497:534	high-capacity uraniumVI (UVI) sorption	497:534	β-Cyclodextrin was intercalated into the mixed-valent iron-based hydroxide layers to synthesize new green rust materials (β-CD GRs), pursuing high-capacity uraniumVI (UVI) sorption.
34419862	3	9	theme	GRs	669:671	arg1	synthesis					651:659	the synthesis	647:659	the synthesis of β-CD GRs	647:671	The molar ratios of FeII to FeIII and the molar ratios of β-CD GR to FeII + FeIII had a significant effect on the synthesis of β-CD GRs.
34419862	10	10	theme	β-CD	1436:1439	arg1	GR					1441:1442	β-CD GR	1436:1442	β-CD GR	1436:1442	The peaks at 1159 and 609 cm-1 vanished with the band at 1103 cm-1 being left-shifted to 1117 cm-1 in the FTIR spectra of β-CD GR during the heterogeneous process.
34419862	8	11	dep	constituents	1135:1146	arg1	mimic					1117:1121	mimic	1117:1121	mimic	1117:1121	The presence of mimic groundwater constituents explicitly deteriorated the interaction between β-CD GR and UVI species.
34419862	6	12	theme	isotherm	956:963	arg1	model					965:969	Langmuir isotherm model	947:969	Langmuir isotherm model	947:969	The pseudo-first-order kinetic model and Langmuir isotherm model were appropriate in fitting the whole adsorption process.
34419862	8	13	theme	groundwater	1123:1133	arg1	constituents					1135:1146	mimic groundwater constituents	1117:1146	mimic groundwater constituents	1117:1146	The presence of mimic groundwater constituents explicitly deteriorated the interaction between β-CD GR and UVI species.
34419862	8	14	theme	β-CD	1196:1199	arg1	GR					1201:1202	β-CD GR	1196:1202	β-CD GR	1196:1202	The presence of mimic groundwater constituents explicitly deteriorated the interaction between β-CD GR and UVI species.
34419862	10	15	theme	FTIR	1420:1423	arg1	spectra					1425:1431	the FTIR spectra	1416:1431	the FTIR spectra of β-CD GR	1416:1442	The peaks at 1159 and 609 cm-1 vanished with the band at 1103 cm-1 being left-shifted to 1117 cm-1 in the FTIR spectra of β-CD GR during the heterogeneous process.
34419862	5	16	theme	strong	820:825	arg1	acidity					827:833	strong acidity	820:833	strong acidity	820:833	Both strong acidity and alkalinity were harmful to the adsorption of β-CD GRs towards UVI.
34419862	1	17	from	influence	146:154	arg1	composition					200:210	composition	200:210	composition	200:210	The influence of intercalated anions on the structure and composition of green rusts supplies a theoretical possibility for the investigation of the structural modification of FeII/FeIII (oxyhydr)oxide materials.
34419862	1	17	from	influence	146:154	arg1	structure					186:194	structure	186:194	structure	186:194	The influence of intercalated anions on the structure and composition of green rusts supplies a theoretical possibility for the investigation of the structural modification of FeII/FeIII (oxyhydr)oxide materials.
34419862	8	18	theme	UVI	1208:1210	arg1	species					1212:1218	UVI species	1208:1218	UVI species	1208:1218	The presence of mimic groundwater constituents explicitly deteriorated the interaction between β-CD GR and UVI species.
34419862	1	19	theme	anions	172:177	arg1	influence					146:154	The influence	142:154	The influence of intercalated anions on the structure and composition of green rusts	142:225	The influence of intercalated anions on the structure and composition of green rusts supplies a theoretical possibility for the investigation of the structural modification of FeII/FeIII (oxyhydr)oxide materials.
34419862	0	20	theme	β-cyclodextrin	106:119	arg1	intercalation					89:101	the intercalation	85:101	the intercalation of β-cyclodextrin into the green rust	85:139	Enhancement of the heterogeneous adsorption and incorporation of uraniumVI caused by the intercalation of β-cyclodextrin into the green rust.
34419862	11	21	theme	unmodified	1656:1665	arg1	GRs					1667:1669	the unmodified GRs	1652:1669	the unmodified GRs	1652:1669	The intercalation of β-CD brought obvious enhancement of UVI species sorption to the GR material, which was combinedly driven by several reaction pathways and different from the unmodified GRs.
34419862	3	22	theme	molar	541:545	arg1	ratios					547:552	The molar ratios	537:552	The molar ratios of FeII to FeIII	537:569	The molar ratios of FeII to FeIII and the molar ratios of β-CD GR to FeII + FeIII had a significant effect on the synthesis of β-CD GRs.
34419862	2	23	theme	iron-based	409:418	arg1	layers					430:435	the mixed-valent iron-based hydroxide layers	392:435	the mixed-valent iron-based hydroxide layers	392:435	β-Cyclodextrin was intercalated into the mixed-valent iron-based hydroxide layers to synthesize new green rust materials (β-CD GRs), pursuing high-capacity uraniumVI (UVI) sorption.
34419862	7	24	theme	GRs	1079:1081	arg1	2548.61 mg/g					1087:1098	2548.61 mg/g	1087:1098	2548.61 mg/g	1087:1098	The maximum monolayer adsorption capacity of β-CD GRs was 2548.61 mg/g.
34419862	7	24	theme	GRs	1079:1081	arg1	capacity					1062:1069	The maximum monolayer adsorption capacity	1029:1069	The maximum monolayer adsorption capacity of β-CD GRs	1029:1081	The maximum monolayer adsorption capacity of β-CD GRs was 2548.61 mg/g.
34419862	9	25	theme	Nanoscale	1221:1229	arg1	nodules					1231:1237	Nanoscale nodules	1221:1237	Nanoscale nodules	1221:1237	Nanoscale nodules and particles were formed on the β-CD GR after the adsorption experiments.
34419862	11	26	from	GRs	1667:1669	arg1	different					1637:1645	different	1637:1645	different	1637:1645	The intercalation of β-CD brought obvious enhancement of UVI species sorption to the GR material, which was combinedly driven by several reaction pathways and different from the unmodified GRs.
34419862	10	27	from	609 cm-1	1336:1343	arg1	peaks					1318:1322	The peaks	1314:1322	The peaks at 1159 and 609 cm-1	1314:1343	The peaks at 1159 and 609 cm-1 vanished with the band at 1103 cm-1 being left-shifted to 1117 cm-1 in the FTIR spectra of β-CD GR during the heterogeneous process.
34419862	2	28	theme	mixed-valent	396:407	arg1	layers					430:435	the mixed-valent iron-based hydroxide layers	392:435	the mixed-valent iron-based hydroxide layers	392:435	β-Cyclodextrin was intercalated into the mixed-valent iron-based hydroxide layers to synthesize new green rust materials (β-CD GRs), pursuing high-capacity uraniumVI (UVI) sorption.
34419862	3	29	theme	β-CD	664:667	arg1	GRs					669:671	β-CD GRs	664:671	β-CD GRs	664:671	The molar ratios of FeII to FeIII and the molar ratios of β-CD GR to FeII + FeIII had a significant effect on the synthesis of β-CD GRs.
34419862	0	30	theme	heterogeneous	19:31	arg1	adsorption					33:42	the heterogeneous adsorption	15:42	the heterogeneous adsorption	15:42	Enhancement of the heterogeneous adsorption and incorporation of uraniumVI caused by the intercalation of β-cyclodextrin into the green rust.
34419862	10	31	theme	GR	1441:1442	arg1	spectra					1425:1431	the FTIR spectra	1416:1431	the FTIR spectra of β-CD GR	1416:1442	The peaks at 1159 and 609 cm-1 vanished with the band at 1103 cm-1 being left-shifted to 1117 cm-1 in the FTIR spectra of β-CD GR during the heterogeneous process.
34419862	2	32	theme	rust	461:464	arg1	materials					466:474	new green rust materials	451:474	new green rust materials (β-CD GRs)	451:485	β-Cyclodextrin was intercalated into the mixed-valent iron-based hydroxide layers to synthesize new green rust materials (β-CD GRs), pursuing high-capacity uraniumVI (UVI) sorption.
34419862	2	32	theme	rust	461:464	arg1	GRs					482:484	β-CD GRs	477:484	β-CD GRs	477:484	β-Cyclodextrin was intercalated into the mixed-valent iron-based hydroxide layers to synthesize new green rust materials (β-CD GRs), pursuing high-capacity uraniumVI (UVI) sorption.
34419862	4	33	theme	composite	782:790	arg1	design					792:797	a central composite design	772:797	a central composite design in combination	772:812	The synthesis process was further optimized by the quadric predictor and desirability function in a central composite design in combination.
34419862	0	34	theme	green	130:134	arg1	rust					136:139	the green rust	126:139	the green rust	126:139	Enhancement of the heterogeneous adsorption and incorporation of uraniumVI caused by the intercalation of β-cyclodextrin into the green rust.
34419862	11	35	theme	species	1539:1545	arg1	sorption					1547:1554	UVI species sorption	1535:1554	UVI species sorption	1535:1554	The intercalation of β-CD brought obvious enhancement of UVI species sorption to the GR material, which was combinedly driven by several reaction pathways and different from the unmodified GRs.
34419862	3	36	theme	FeII	557:560	arg1	ratios					547:552	The molar ratios	537:552	The molar ratios of FeII to FeIII	537:569	The molar ratios of FeII to FeIII and the molar ratios of β-CD GR to FeII + FeIII had a significant effect on the synthesis of β-CD GRs.
34419862	3	36	theme	FeII	557:560	arg1	ratios					585:590	the molar ratios	575:590	the molar ratios of β-CD GR to FeII + FeIII	575:617	The molar ratios of FeII to FeIII and the molar ratios of β-CD GR to FeII + FeIII had a significant effect on the synthesis of β-CD GRs.
34419862	10	37	theme	heterogeneous	1455:1467	arg1	process					1469:1475	the heterogeneous process	1451:1475	the heterogeneous process	1451:1475	The peaks at 1159 and 609 cm-1 vanished with the band at 1103 cm-1 being left-shifted to 1117 cm-1 in the FTIR spectra of β-CD GR during the heterogeneous process.
34419862	2	38	theme	new	451:453	arg1	materials					466:474	new green rust materials	451:474	new green rust materials (β-CD GRs)	451:485	β-Cyclodextrin was intercalated into the mixed-valent iron-based hydroxide layers to synthesize new green rust materials (β-CD GRs), pursuing high-capacity uraniumVI (UVI) sorption.
34419862	2	38	theme	new	451:453	arg1	GRs					482:484	β-CD GRs	477:484	β-CD GRs	477:484	β-Cyclodextrin was intercalated into the mixed-valent iron-based hydroxide layers to synthesize new green rust materials (β-CD GRs), pursuing high-capacity uraniumVI (UVI) sorption.
34419862	2	39	theme	green	455:459	arg1	materials					466:474	new green rust materials	451:474	new green rust materials (β-CD GRs)	451:485	β-Cyclodextrin was intercalated into the mixed-valent iron-based hydroxide layers to synthesize new green rust materials (β-CD GRs), pursuing high-capacity uraniumVI (UVI) sorption.
34419862	2	39	theme	green	455:459	arg1	GRs					482:484	β-CD GRs	477:484	β-CD GRs	477:484	β-Cyclodextrin was intercalated into the mixed-valent iron-based hydroxide layers to synthesize new green rust materials (β-CD GRs), pursuing high-capacity uraniumVI (UVI) sorption.
34419862	1	40	theme	structural	291:300	arg1	modification					302:313	the structural modification	287:313	the structural modification of FeII/FeIII (oxyhydr)oxide materials	287:352	The influence of intercalated anions on the structure and composition of green rusts supplies a theoretical possibility for the investigation of the structural modification of FeII/FeIII (oxyhydr)oxide materials.
34419862	7	41	theme	β-CD	1074:1077	arg1	GRs					1079:1081	β-CD GRs	1074:1081	β-CD GRs	1074:1081	The maximum monolayer adsorption capacity of β-CD GRs was 2548.61 mg/g.
34419862	3	42	theme	significant	625:635	arg1	effect					637:642	a significant effect	623:642	a significant effect	623:642	The molar ratios of FeII to FeIII and the molar ratios of β-CD GR to FeII + FeIII had a significant effect on the synthesis of β-CD GRs.
34419862	5	43	theme	β-CD	884:887	arg1	GRs					889:891	β-CD GRs	884:891	β-CD GRs	884:891	Both strong acidity and alkalinity were harmful to the adsorption of β-CD GRs towards UVI.
34419862	3	44	contain	had	619:621	arg1	ratios					547:552	The molar ratios	537:552	The molar ratios of FeII to FeIII	537:569	The molar ratios of FeII to FeIII and the molar ratios of β-CD GR to FeII + FeIII had a significant effect on the synthesis of β-CD GRs.
34419862	3	44	contain	had	619:621	arg1	ratios					585:590	the molar ratios	575:590	the molar ratios of β-CD GR to FeII + FeIII	575:617	The molar ratios of FeII to FeIII and the molar ratios of β-CD GR to FeII + FeIII had a significant effect on the synthesis of β-CD GRs.
34419862	3	44	contain	had	619:621	arg2	effect					637:642	a significant effect	623:642	a significant effect	623:642	The molar ratios of FeII to FeIII and the molar ratios of β-CD GR to FeII + FeIII had a significant effect on the synthesis of β-CD GRs.
34419862	1	45	theme	modification	302:313	arg1	investigation					270:282	the investigation	266:282	the investigation of the structural modification of FeII/FeIII (oxyhydr)oxide materials	266:352	The influence of intercalated anions on the structure and composition of green rusts supplies a theoretical possibility for the investigation of the structural modification of FeII/FeIII (oxyhydr)oxide materials.
34419862	7	46	theme	adsorption	1051:1060	arg1	2548.61 mg/g					1087:1098	2548.61 mg/g	1087:1098	2548.61 mg/g	1087:1098	The maximum monolayer adsorption capacity of β-CD GRs was 2548.61 mg/g.
34419862	7	46	theme	adsorption	1051:1060	arg1	capacity					1062:1069	The maximum monolayer adsorption capacity	1029:1069	The maximum monolayer adsorption capacity of β-CD GRs	1029:1081	The maximum monolayer adsorption capacity of β-CD GRs was 2548.61 mg/g.
34419862	4	47	theme	central	774:780	arg1	design					792:797	a central composite design	772:797	a central composite design in combination	772:812	The synthesis process was further optimized by the quadric predictor and desirability function in a central composite design in combination.
34419862	10	48	from	1103 cm-1	1371:1379	arg1	band					1363:1366	the band	1359:1366	the band at 1103 cm-1	1359:1379	The peaks at 1159 and 609 cm-1 vanished with the band at 1103 cm-1 being left-shifted to 1117 cm-1 in the FTIR spectra of β-CD GR during the heterogeneous process.
34419862	10	48	from	1103 cm-1	1371:1379	arg1	left-shifted					1387:1398	left-shifted	1387:1398	left-shifted	1387:1398	The peaks at 1159 and 609 cm-1 vanished with the band at 1103 cm-1 being left-shifted to 1117 cm-1 in the FTIR spectra of β-CD GR during the heterogeneous process.
34419862	0	49	theme	adsorption	33:42	arg1	Enhancement					0:10	Enhancement	0:10	Enhancement of the heterogeneous adsorption and incorporation of uraniumVI	0:73	Enhancement of the heterogeneous adsorption and incorporation of uraniumVI caused by the intercalation of β-cyclodextrin into the green rust.
34419862	1	50	dep	structure	186:194	arg1	the					182:184	the	182:184	the	182:184	The influence of intercalated anions on the structure and composition of green rusts supplies a theoretical possibility for the investigation of the structural modification of FeII/FeIII (oxyhydr)oxide materials.
34419862	1	51	theme	green	215:219	arg1	rusts					221:225	green rusts	215:225	green rusts	215:225	The influence of intercalated anions on the structure and composition of green rusts supplies a theoretical possibility for the investigation of the structural modification of FeII/FeIII (oxyhydr)oxide materials.
34419862	1	52	theme	FeII/FeIII	318:327	arg1	materials					344:352	FeII/FeIII (oxyhydr)oxide materials	318:352	FeII/FeIII (oxyhydr)oxide materials	318:352	The influence of intercalated anions on the structure and composition of green rusts supplies a theoretical possibility for the investigation of the structural modification of FeII/FeIII (oxyhydr)oxide materials.
34419862	7	53	theme	maximum	1033:1039	arg1	2548.61 mg/g					1087:1098	2548.61 mg/g	1087:1098	2548.61 mg/g	1087:1098	The maximum monolayer adsorption capacity of β-CD GRs was 2548.61 mg/g.
34419862	7	53	theme	maximum	1033:1039	arg1	capacity					1062:1069	The maximum monolayer adsorption capacity	1029:1069	The maximum monolayer adsorption capacity of β-CD GRs	1029:1081	The maximum monolayer adsorption capacity of β-CD GRs was 2548.61 mg/g.
34419862	2	54	theme	uraniumVI	511:519	arg1	sorption					527:534	high-capacity uraniumVI (UVI) sorption	497:534	high-capacity uraniumVI (UVI) sorption	497:534	β-Cyclodextrin was intercalated into the mixed-valent iron-based hydroxide layers to synthesize new green rust materials (β-CD GRs), pursuing high-capacity uraniumVI (UVI) sorption.
34419862	5	55	theme	GRs	889:891	arg1	adsorption					870:879	the adsorption	866:879	the adsorption of β-CD GRs towards UVI	866:903	Both strong acidity and alkalinity were harmful to the adsorption of β-CD GRs towards UVI.
34419862	0	56	theme	incorporation	48:60	arg1	Enhancement					0:10	Enhancement	0:10	Enhancement of the heterogeneous adsorption and incorporation of uraniumVI	0:73	Enhancement of the heterogeneous adsorption and incorporation of uraniumVI caused by the intercalation of β-cyclodextrin into the green rust.
34419862	6	57	theme	pseudo-first-order	910:927	arg1	model					937:941	The pseudo-first-order kinetic model	906:941	The pseudo-first-order kinetic model	906:941	The pseudo-first-order kinetic model and Langmuir isotherm model were appropriate in fitting the whole adsorption process.
34419862	6	57	theme	pseudo-first-order	910:927	arg1	appropriate					976:986	appropriate	976:986	appropriate	976:986	The pseudo-first-order kinetic model and Langmuir isotherm model were appropriate in fitting the whole adsorption process.
34419862	7	58	theme	monolayer	1041:1049	arg1	2548.61 mg/g					1087:1098	2548.61 mg/g	1087:1098	2548.61 mg/g	1087:1098	The maximum monolayer adsorption capacity of β-CD GRs was 2548.61 mg/g.
34419862	7	58	theme	monolayer	1041:1049	arg1	capacity					1062:1069	The maximum monolayer adsorption capacity	1029:1069	The maximum monolayer adsorption capacity of β-CD GRs	1029:1081	The maximum monolayer adsorption capacity of β-CD GRs was 2548.61 mg/g.
34419862	2	59	theme	β-CD	477:480	arg1	materials					466:474	new green rust materials	451:474	new green rust materials (β-CD GRs)	451:485	β-Cyclodextrin was intercalated into the mixed-valent iron-based hydroxide layers to synthesize new green rust materials (β-CD GRs), pursuing high-capacity uraniumVI (UVI) sorption.
34419862	2	59	theme	β-CD	477:480	arg1	GRs					482:484	β-CD GRs	477:484	β-CD GRs	477:484	β-Cyclodextrin was intercalated into the mixed-valent iron-based hydroxide layers to synthesize new green rust materials (β-CD GRs), pursuing high-capacity uraniumVI (UVI) sorption.
34419862	3	60	theme	molar	579:583	arg1	ratios					585:590	the molar ratios	575:590	the molar ratios of β-CD GR to FeII + FeIII	575:617	The molar ratios of FeII to FeIII and the molar ratios of β-CD GR to FeII + FeIII had a significant effect on the synthesis of β-CD GRs.
34419862	9	61	theme	adsorption	1290:1299	arg1	experiments					1301:1311	the adsorption experiments	1286:1311	the adsorption experiments	1286:1311	Nanoscale nodules and particles were formed on the β-CD GR after the adsorption experiments.
34419862	4	62	theme	synthesis	678:686	arg1	process					688:694	The synthesis process	674:694	The synthesis process	674:694	The synthesis process was further optimized by the quadric predictor and desirability function in a central composite design in combination.
34419862	1	63	theme	oxyhydr	330:336	arg1	materials					344:352	FeII/FeIII (oxyhydr)oxide materials	318:352	FeII/FeIII (oxyhydr)oxide materials	318:352	The influence of intercalated anions on the structure and composition of green rusts supplies a theoretical possibility for the investigation of the structural modification of FeII/FeIII (oxyhydr)oxide materials.
34419862	9	64	theme	β-CD	1272:1275	arg1	GR					1277:1278	the β-CD GR	1268:1278	the β-CD GR	1268:1278	Nanoscale nodules and particles were formed on the β-CD GR after the adsorption experiments.
34419862	4	65	from	design	792:797	arg1	combination					802:812	combination	802:812	combination	802:812	The synthesis process was further optimized by the quadric predictor and desirability function in a central composite design in combination.
34419862	10	66	from	1117 cm-1	1403:1411	arg1	spectra					1425:1431	the FTIR spectra	1416:1431	the FTIR spectra of β-CD GR	1416:1442	The peaks at 1159 and 609 cm-1 vanished with the band at 1103 cm-1 being left-shifted to 1117 cm-1 in the FTIR spectra of β-CD GR during the heterogeneous process.
34419862	4	67	theme	quadric	725:731	arg1	predictor					733:741	the quadric predictor	721:741	the quadric predictor	721:741	The synthesis process was further optimized by the quadric predictor and desirability function in a central composite design in combination.
34419862	1	68	theme	rusts	221:225	arg1	composition					200:210	composition	200:210	composition	200:210	The influence of intercalated anions on the structure and composition of green rusts supplies a theoretical possibility for the investigation of the structural modification of FeII/FeIII (oxyhydr)oxide materials.
34419862	1	68	theme	rusts	221:225	arg1	structure					186:194	structure	186:194	structure	186:194	The influence of intercalated anions on the structure and composition of green rusts supplies a theoretical possibility for the investigation of the structural modification of FeII/FeIII (oxyhydr)oxide materials.
34419862	4	69	theme	desirability	747:758	arg1	function					760:767	desirability function	747:767	desirability function	747:767	The synthesis process was further optimized by the quadric predictor and desirability function in a central composite design in combination.
34419862	11	70	theme	reaction	1615:1622	arg1	pathways					1624:1631	several reaction pathways	1607:1631	several reaction pathways	1607:1631	The intercalation of β-CD brought obvious enhancement of UVI species sorption to the GR material, which was combinedly driven by several reaction pathways and different from the unmodified GRs.
34419862	6	71	theme	adsorption	1009:1018	arg1	process					1020:1026	the whole adsorption process	999:1026	the whole adsorption process	999:1026	The pseudo-first-order kinetic model and Langmuir isotherm model were appropriate in fitting the whole adsorption process.
34419862	8	72	theme	constituents	1135:1146	arg1	presence					1105:1112	The presence	1101:1112	The presence of mimic groundwater constituents	1101:1146	The presence of mimic groundwater constituents explicitly deteriorated the interaction between β-CD GR and UVI species.
34419862	11	73	theme	obvious	1512:1518	arg1	enhancement					1520:1530	obvious enhancement	1512:1530	obvious enhancement of UVI species sorption to the GR material, which was combinedly driven by several reaction pathways and different from the unmodified GRs	1512:1669	The intercalation of β-CD brought obvious enhancement of UVI species sorption to the GR material, which was combinedly driven by several reaction pathways and different from the unmodified GRs.
34419862	3	74	theme	β-CD	595:598	arg1	GR					600:601	β-CD GR	595:601	β-CD GR	595:601	The molar ratios of FeII to FeIII and the molar ratios of β-CD GR to FeII + FeIII had a significant effect on the synthesis of β-CD GRs.
34419862	11	75	theme	β-CD	1499:1502	arg1	intercalation					1482:1494	The intercalation	1478:1494	The intercalation of β-CD	1478:1502	The intercalation of β-CD brought obvious enhancement of UVI species sorption to the GR material, which was combinedly driven by several reaction pathways and different from the unmodified GRs.
34419862	10	76	from	1159	1327:1330	arg1	peaks					1318:1322	The peaks	1314:1322	The peaks at 1159 and 609 cm-1	1314:1343	The peaks at 1159 and 609 cm-1 vanished with the band at 1103 cm-1 being left-shifted to 1117 cm-1 in the FTIR spectra of β-CD GR during the heterogeneous process.
34419862	6	77	theme	whole	1003:1007	arg1	process					1020:1026	the whole adsorption process	999:1026	the whole adsorption process	999:1026	The pseudo-first-order kinetic model and Langmuir isotherm model were appropriate in fitting the whole adsorption process.
34419862	0	78	theme	uraniumVI	65:73	arg1	Enhancement					0:10	Enhancement	0:10	Enhancement of the heterogeneous adsorption and incorporation of uraniumVI	0:73	Enhancement of the heterogeneous adsorption and incorporation of uraniumVI caused by the intercalation of β-cyclodextrin into the green rust.
34419862	11	79	theme	GR	1563:1564	arg1	driven					1597:1602	driven	1597:1602	combinedly driven by several reaction pathways	1586:1631	The intercalation of β-CD brought obvious enhancement of UVI species sorption to the GR material, which was combinedly driven by several reaction pathways and different from the unmodified GRs.
34419862	11	79	theme	GR	1563:1564	arg1	material					1566:1573	the GR material	1559:1573	the GR material	1559:1573	The intercalation of β-CD brought obvious enhancement of UVI species sorption to the GR material, which was combinedly driven by several reaction pathways and different from the unmodified GRs.
34419862	3	80	theme	GR	600:601	arg1	ratios					547:552	The molar ratios	537:552	The molar ratios of FeII to FeIII	537:569	The molar ratios of FeII to FeIII and the molar ratios of β-CD GR to FeII + FeIII had a significant effect on the synthesis of β-CD GRs.
34419862	3	80	theme	GR	600:601	arg1	ratios					585:590	the molar ratios	575:590	the molar ratios of β-CD GR to FeII + FeIII	575:617	The molar ratios of FeII to FeIII and the molar ratios of β-CD GR to FeII + FeIII had a significant effect on the synthesis of β-CD GRs.
34419862	6	81	theme	kinetic	929:935	arg1	model					937:941	The pseudo-first-order kinetic model	906:941	The pseudo-first-order kinetic model	906:941	The pseudo-first-order kinetic model and Langmuir isotherm model were appropriate in fitting the whole adsorption process.
34419862	6	81	theme	kinetic	929:935	arg1	appropriate					976:986	appropriate	976:986	appropriate	976:986	The pseudo-first-order kinetic model and Langmuir isotherm model were appropriate in fitting the whole adsorption process.
34419862	1	82	theme	theoretical	238:248	arg1	possibility					250:260	a theoretical possibility	236:260	a theoretical possibility for the investigation of the structural modification of FeII/FeIII (oxyhydr)oxide materials	236:352	The influence of intercalated anions on the structure and composition of green rusts supplies a theoretical possibility for the investigation of the structural modification of FeII/FeIII (oxyhydr)oxide materials.
34047310	5	0	theme	antibacterial	742:754	arg1	tests					756:760	In vitro and in vivo antibacterial tests	721:760	In vitro and in vivo antibacterial tests	721:760	In vitro and in vivo antibacterial tests showed that the HPCH/TA/Fe hydrogel possessed an effective and rapid bactericidal effect with 10 minutes of near-infrared laser irradiation.
34047310	4	1	theme	low	679:681	arg1	dosage					683:688	a low dosage	677:688	a low dosage of the antibacterial agent TA	677:718	The composite hydrogel possessed good cytocompatibility and hemocompatibility with a low dosage of the antibacterial agent TA.
34047310	7	2	theme	composite	1123:1131	arg1	hydrogel					1133:1140	the injectable photothermally active antibacterial composite hydrogel	1072:1140	the injectable photothermally active antibacterial composite hydrogel	1072:1140	Thus, the injectable photothermally active antibacterial composite hydrogel has great potential for the infected skin wound regeneration in clinical applications.
34047310	5	3	dep	In	721:722	arg1	vitro					724:728	vitro	724:728	vitro	724:728	In vitro and in vivo antibacterial tests showed that the HPCH/TA/Fe hydrogel possessed an effective and rapid bactericidal effect with 10 minutes of near-infrared laser irradiation.
34047310	5	4	theme	irradiation	890:900	arg1	minutes					859:865	10 minutes	856:865	10 minutes of near-infrared laser irradiation	856:900	In vitro and in vivo antibacterial tests showed that the HPCH/TA/Fe hydrogel possessed an effective and rapid bactericidal effect with 10 minutes of near-infrared laser irradiation.
34047310	6	5	with	combination	920:930	arg1	hydrogel					970:977	the hydrogel	966:977	the hydrogel	966:977	Furthermore, the combination of a low-level laser therapy with the hydrogel is conducive to the acceleration of wound closure and promotion of skin tissue repair.
34047310	1	6	theme	wound	277:281	arg1	process					291:297	the wound healing process	273:297	the wound healing process	273:297	Prevention of bacterial infection, acceleration of wound closure and promotion of skin regeneration are crucial in the wound healing process.
34047310	7	7	from	potential	1152:1160	arg1	applications					1215:1226	clinical applications	1206:1226	clinical applications	1206:1226	Thus, the injectable photothermally active antibacterial composite hydrogel has great potential for the infected skin wound regeneration in clinical applications.
34047310	2	8	theme	hydrogel	383:390	arg1	activity					331:338	the photothermal activity	314:338	the photothermal activity of an injectable thermosensitive composite hydrogel based on hydroxypropyl chitin (HPCH), tannic acid (TA) and ferric ions (Fe3+)	314:468	In this work, the photothermal activity of an injectable thermosensitive composite hydrogel based on hydroxypropyl chitin (HPCH), tannic acid (TA) and ferric ions (Fe3+) was studied.
34047310	1	9	theme	healing	283:289	arg1	process					291:297	the wound healing process	273:297	the wound healing process	273:297	Prevention of bacterial infection, acceleration of wound closure and promotion of skin regeneration are crucial in the wound healing process.
34047310	0	10	theme	healing	114:120	arg1	process					122:128	the wound healing process	104:128	the wound healing process	104:128	An injectable photothermally active antibacterial composite hydroxypropyl chitin hydrogel for promoting the wound healing process through photobiomodulation.
34047310	7	11	theme	active	1102:1107	arg1	hydrogel					1133:1140	the injectable photothermally active antibacterial composite hydrogel	1072:1140	the injectable photothermally active antibacterial composite hydrogel	1072:1140	Thus, the injectable photothermally active antibacterial composite hydrogel has great potential for the infected skin wound regeneration in clinical applications.
34047310	7	12	contain	has	1142:1144	arg2	potential					1152:1160	great potential	1146:1160	great potential for the infected skin wound regeneration in clinical applications	1146:1226	Thus, the injectable photothermally active antibacterial composite hydrogel has great potential for the infected skin wound regeneration in clinical applications.
34047310	7	12	contain	has	1142:1144	arg1	hydrogel					1133:1140	the injectable photothermally active antibacterial composite hydrogel	1072:1140	the injectable photothermally active antibacterial composite hydrogel	1072:1140	Thus, the injectable photothermally active antibacterial composite hydrogel has great potential for the infected skin wound regeneration in clinical applications.
34047310	5	13	theme	laser	884:888	arg1	irradiation					890:900	near-infrared laser irradiation	870:900	near-infrared laser irradiation	870:900	In vitro and in vivo antibacterial tests showed that the HPCH/TA/Fe hydrogel possessed an effective and rapid bactericidal effect with 10 minutes of near-infrared laser irradiation.
34047310	0	14	theme	wound	108:112	arg1	process					122:128	the wound healing process	104:128	the wound healing process	104:128	An injectable photothermally active antibacterial composite hydroxypropyl chitin hydrogel for promoting the wound healing process through photobiomodulation.
34047310	7	15	theme	antibacterial	1109:1121	arg1	hydrogel					1133:1140	the injectable photothermally active antibacterial composite hydrogel	1072:1140	the injectable photothermally active antibacterial composite hydrogel	1072:1140	Thus, the injectable photothermally active antibacterial composite hydrogel has great potential for the infected skin wound regeneration in clinical applications.
34047310	4	16	theme	good	627:630	arg1	cytocompatibility					632:648	good cytocompatibility	627:648	good cytocompatibility	627:648	The composite hydrogel possessed good cytocompatibility and hemocompatibility with a low dosage of the antibacterial agent TA.
34047310	5	17	theme	bactericidal	831:842	arg1	effect					844:849	an effective and rapid bactericidal effect	808:849	an effective and rapid bactericidal effect	808:849	In vitro and in vivo antibacterial tests showed that the HPCH/TA/Fe hydrogel possessed an effective and rapid bactericidal effect with 10 minutes of near-infrared laser irradiation.
34047310	4	18	theme	antibacterial	697:709	arg1	agent					711:715	the antibacterial agent TA	693:718	the antibacterial agent TA	693:718	The composite hydrogel possessed good cytocompatibility and hemocompatibility with a low dosage of the antibacterial agent TA.
34047310	5	19	theme	rapid	825:829	arg1	effect					844:849	an effective and rapid bactericidal effect	808:849	an effective and rapid bactericidal effect	808:849	In vitro and in vivo antibacterial tests showed that the HPCH/TA/Fe hydrogel possessed an effective and rapid bactericidal effect with 10 minutes of near-infrared laser irradiation.
34047310	4	20	theme	composite	598:606	arg1	hydrogel					608:615	The composite hydrogel	594:615	The composite hydrogel	594:615	The composite hydrogel possessed good cytocompatibility and hemocompatibility with a low dosage of the antibacterial agent TA.
34047310	3	21	theme	photothermal	505:516	arg1	efficiency					518:527	the photothermal efficiency	501:527	the photothermal efficiency	501:527	It was found that the photothermal efficiency was enhanced when the molar ratio of Fe3+/TA increased up to 20.
34047310	6	22	theme	repair	1058:1063	arg1	promotion					1033:1041	promotion	1033:1041	promotion of skin tissue repair	1033:1063	Furthermore, the combination of a low-level laser therapy with the hydrogel is conducive to the acceleration of wound closure and promotion of skin tissue repair.
34047310	6	22	theme	repair	1058:1063	arg1	acceleration					999:1010	the acceleration	995:1010	the acceleration of wound closure	995:1027	Furthermore, the combination of a low-level laser therapy with the hydrogel is conducive to the acceleration of wound closure and promotion of skin tissue repair.
34047310	5	23	theme	HPCH/TA/Fe	778:787	arg1	hydrogel					789:796	the HPCH/TA/Fe hydrogel	774:796	the HPCH/TA/Fe hydrogel	774:796	In vitro and in vivo antibacterial tests showed that the HPCH/TA/Fe hydrogel possessed an effective and rapid bactericidal effect with 10 minutes of near-infrared laser irradiation.
34047310	7	24	theme	clinical	1206:1213	arg1	applications					1215:1226	clinical applications	1206:1226	clinical applications	1206:1226	Thus, the injectable photothermally active antibacterial composite hydrogel has great potential for the infected skin wound regeneration in clinical applications.
34047310	2	25	theme	ferric	451:456	arg1	Fe3+					464:467	Fe3+	464:467	Fe3+	464:467	In this work, the photothermal activity of an injectable thermosensitive composite hydrogel based on hydroxypropyl chitin (HPCH), tannic acid (TA) and ferric ions (Fe3+) was studied.
34047310	2	25	theme	ferric	451:456	arg1	ions					458:461	ferric ions	451:461	ferric ions (Fe3+)	451:468	In this work, the photothermal activity of an injectable thermosensitive composite hydrogel based on hydroxypropyl chitin (HPCH), tannic acid (TA) and ferric ions (Fe3+) was studied.
34047310	1	26	theme	wound	209:213	arg1	closure					215:221	wound closure	209:221	wound closure	209:221	Prevention of bacterial infection, acceleration of wound closure and promotion of skin regeneration are crucial in the wound healing process.
34047310	0	27	theme	injectable	3:12	arg1	hydrogel					81:88	An injectable photothermally active antibacterial composite hydroxypropyl chitin hydrogel	0:88	An injectable photothermally active antibacterial composite hydroxypropyl chitin hydrogel for promoting the wound healing process through photobiomodulation.	0:156	An injectable photothermally active antibacterial composite hydroxypropyl chitin hydrogel for promoting the wound healing process through photobiomodulation.
34047310	3	28	theme	molar	551:555	arg1	ratio					557:561	the molar ratio	547:561	the molar ratio of Fe3+/TA	547:572	It was found that the photothermal efficiency was enhanced when the molar ratio of Fe3+/TA increased up to 20.
34047310	7	29	theme	skin	1179:1182	arg1	regeneration					1190:1201	the infected skin wound regeneration	1166:1201	the infected skin wound regeneration	1166:1201	Thus, the injectable photothermally active antibacterial composite hydrogel has great potential for the infected skin wound regeneration in clinical applications.
34047310	1	30	theme	closure	215:221	arg1	Prevention					158:167	Prevention	158:167	Prevention of bacterial infection	158:190	Prevention of bacterial infection, acceleration of wound closure and promotion of skin regeneration are crucial in the wound healing process.
34047310	1	30	theme	closure	215:221	arg1	promotion					227:235	promotion	227:235	promotion of skin regeneration	227:256	Prevention of bacterial infection, acceleration of wound closure and promotion of skin regeneration are crucial in the wound healing process.
34047310	1	30	theme	closure	215:221	arg1	acceleration					193:204	acceleration	193:204	acceleration of wound closure	193:221	Prevention of bacterial infection, acceleration of wound closure and promotion of skin regeneration are crucial in the wound healing process.
34047310	7	31	theme	wound	1184:1188	arg1	regeneration					1190:1201	the infected skin wound regeneration	1166:1201	the infected skin wound regeneration	1166:1201	Thus, the injectable photothermally active antibacterial composite hydrogel has great potential for the infected skin wound regeneration in clinical applications.
34047310	7	32	theme	injectable	1076:1085	arg1	hydrogel					1133:1140	the injectable photothermally active antibacterial composite hydrogel	1072:1140	the injectable photothermally active antibacterial composite hydrogel	1072:1140	Thus, the injectable photothermally active antibacterial composite hydrogel has great potential for the infected skin wound regeneration in clinical applications.
34047310	6	33	theme	tissue	1051:1056	arg1	repair					1058:1063	skin tissue repair	1046:1063	skin tissue repair	1046:1063	Furthermore, the combination of a low-level laser therapy with the hydrogel is conducive to the acceleration of wound closure and promotion of skin tissue repair.
34047310	0	34	theme	active	29:34	arg1	hydrogel					81:88	An injectable photothermally active antibacterial composite hydroxypropyl chitin hydrogel	0:88	An injectable photothermally active antibacterial composite hydroxypropyl chitin hydrogel for promoting the wound healing process through photobiomodulation.	0:156	An injectable photothermally active antibacterial composite hydroxypropyl chitin hydrogel for promoting the wound healing process through photobiomodulation.
34047310	1	35	from	process	291:297	arg1	crucial					262:268	crucial	262:268	crucial	262:268	Prevention of bacterial infection, acceleration of wound closure and promotion of skin regeneration are crucial in the wound healing process.
34047310	2	36	theme	photothermal	318:329	arg1	activity					331:338	the photothermal activity	314:338	the photothermal activity of an injectable thermosensitive composite hydrogel based on hydroxypropyl chitin (HPCH), tannic acid (TA) and ferric ions (Fe3+)	314:468	In this work, the photothermal activity of an injectable thermosensitive composite hydrogel based on hydroxypropyl chitin (HPCH), tannic acid (TA) and ferric ions (Fe3+) was studied.
34047310	6	37	theme	skin	1046:1049	arg1	repair					1058:1063	skin tissue repair	1046:1063	skin tissue repair	1046:1063	Furthermore, the combination of a low-level laser therapy with the hydrogel is conducive to the acceleration of wound closure and promotion of skin tissue repair.
34047310	5	38	theme	effective	811:819	arg1	effect					844:849	an effective and rapid bactericidal effect	808:849	an effective and rapid bactericidal effect	808:849	In vitro and in vivo antibacterial tests showed that the HPCH/TA/Fe hydrogel possessed an effective and rapid bactericidal effect with 10 minutes of near-infrared laser irradiation.
34047310	4	39	contain	possessed	617:625	arg2	hemocompatibility					654:670	hemocompatibility	654:670	hemocompatibility	654:670	The composite hydrogel possessed good cytocompatibility and hemocompatibility with a low dosage of the antibacterial agent TA.
34047310	4	39	contain	possessed	617:625	arg1	hydrogel					608:615	The composite hydrogel	594:615	The composite hydrogel	594:615	The composite hydrogel possessed good cytocompatibility and hemocompatibility with a low dosage of the antibacterial agent TA.
34047310	4	39	contain	possessed	617:625	arg2	cytocompatibility					632:648	good cytocompatibility	627:648	good cytocompatibility	627:648	The composite hydrogel possessed good cytocompatibility and hemocompatibility with a low dosage of the antibacterial agent TA.
34047310	2	40	theme	hydroxypropyl	401:413	arg1	HPCH					423:426	HPCH	423:426	HPCH	423:426	In this work, the photothermal activity of an injectable thermosensitive composite hydrogel based on hydroxypropyl chitin (HPCH), tannic acid (TA) and ferric ions (Fe3+) was studied.
34047310	2	40	theme	hydroxypropyl	401:413	arg1	chitin					415:420	hydroxypropyl chitin	401:420	hydroxypropyl chitin (HPCH)	401:427	In this work, the photothermal activity of an injectable thermosensitive composite hydrogel based on hydroxypropyl chitin (HPCH), tannic acid (TA) and ferric ions (Fe3+) was studied.
34047310	0	41	theme	composite	50:58	arg1	hydrogel					81:88	An injectable photothermally active antibacterial composite hydroxypropyl chitin hydrogel	0:88	An injectable photothermally active antibacterial composite hydroxypropyl chitin hydrogel for promoting the wound healing process through photobiomodulation.	0:156	An injectable photothermally active antibacterial composite hydroxypropyl chitin hydrogel for promoting the wound healing process through photobiomodulation.
34047310	6	42	theme	therapy	953:959	arg1	conducive					982:990	conducive	982:990	conducive	982:990	Furthermore, the combination of a low-level laser therapy with the hydrogel is conducive to the acceleration of wound closure and promotion of skin tissue repair.
34047310	6	42	theme	therapy	953:959	arg1	combination					920:930	the combination	916:930	the combination of a low-level laser therapy with the hydrogel	916:977	Furthermore, the combination of a low-level laser therapy with the hydrogel is conducive to the acceleration of wound closure and promotion of skin tissue repair.
34047310	5	43	dep	in	734:735	arg1	vivo					737:740	vivo	737:740	vivo	737:740	In vitro and in vivo antibacterial tests showed that the HPCH/TA/Fe hydrogel possessed an effective and rapid bactericidal effect with 10 minutes of near-infrared laser irradiation.
34047310	2	44	theme	tannic	430:435	arg1	TA					443:444	TA	443:444	TA	443:444	In this work, the photothermal activity of an injectable thermosensitive composite hydrogel based on hydroxypropyl chitin (HPCH), tannic acid (TA) and ferric ions (Fe3+) was studied.
34047310	2	44	theme	tannic	430:435	arg1	acid					437:440	tannic acid	430:440	tannic acid (TA)	430:445	In this work, the photothermal activity of an injectable thermosensitive composite hydrogel based on hydroxypropyl chitin (HPCH), tannic acid (TA) and ferric ions (Fe3+) was studied.
34047310	1	45	theme	skin	240:243	arg1	regeneration					245:256	skin regeneration	240:256	skin regeneration	240:256	Prevention of bacterial infection, acceleration of wound closure and promotion of skin regeneration are crucial in the wound healing process.
34047310	0	46	theme	antibacterial	36:48	arg1	hydrogel					81:88	An injectable photothermally active antibacterial composite hydroxypropyl chitin hydrogel	0:88	An injectable photothermally active antibacterial composite hydroxypropyl chitin hydrogel for promoting the wound healing process through photobiomodulation.	0:156	An injectable photothermally active antibacterial composite hydroxypropyl chitin hydrogel for promoting the wound healing process through photobiomodulation.
34047310	6	47	theme	laser	947:951	arg1	therapy					953:959	a low-level laser therapy	935:959	a low-level laser therapy	935:959	Furthermore, the combination of a low-level laser therapy with the hydrogel is conducive to the acceleration of wound closure and promotion of skin tissue repair.
34047310	5	48	contain	possessed	798:806	arg2	effect					844:849	an effective and rapid bactericidal effect	808:849	an effective and rapid bactericidal effect	808:849	In vitro and in vivo antibacterial tests showed that the HPCH/TA/Fe hydrogel possessed an effective and rapid bactericidal effect with 10 minutes of near-infrared laser irradiation.
34047310	5	48	contain	possessed	798:806	arg1	hydrogel					789:796	the HPCH/TA/Fe hydrogel	774:796	the HPCH/TA/Fe hydrogel	774:796	In vitro and in vivo antibacterial tests showed that the HPCH/TA/Fe hydrogel possessed an effective and rapid bactericidal effect with 10 minutes of near-infrared laser irradiation.
34047310	1	49	theme	regeneration	245:256	arg1	Prevention					158:167	Prevention	158:167	Prevention of bacterial infection	158:190	Prevention of bacterial infection, acceleration of wound closure and promotion of skin regeneration are crucial in the wound healing process.
34047310	1	49	theme	regeneration	245:256	arg1	promotion					227:235	promotion	227:235	promotion of skin regeneration	227:256	Prevention of bacterial infection, acceleration of wound closure and promotion of skin regeneration are crucial in the wound healing process.
34047310	1	49	theme	regeneration	245:256	arg1	acceleration					193:204	acceleration	193:204	acceleration of wound closure	193:221	Prevention of bacterial infection, acceleration of wound closure and promotion of skin regeneration are crucial in the wound healing process.
34047310	0	50	theme	chitin	74:79	arg1	hydrogel					81:88	An injectable photothermally active antibacterial composite hydroxypropyl chitin hydrogel	0:88	An injectable photothermally active antibacterial composite hydroxypropyl chitin hydrogel for promoting the wound healing process through photobiomodulation.	0:156	An injectable photothermally active antibacterial composite hydroxypropyl chitin hydrogel for promoting the wound healing process through photobiomodulation.
34047310	5	51	theme	near-infrared	870:882	arg1	irradiation					890:900	near-infrared laser irradiation	870:900	near-infrared laser irradiation	870:900	In vitro and in vivo antibacterial tests showed that the HPCH/TA/Fe hydrogel possessed an effective and rapid bactericidal effect with 10 minutes of near-infrared laser irradiation.
34047310	2	52	theme	composite	373:381	arg1	hydrogel					383:390	an injectable thermosensitive composite hydrogel	343:390	an injectable thermosensitive composite hydrogel based on hydroxypropyl chitin (HPCH), tannic acid (TA) and ferric ions (Fe3+)	343:468	In this work, the photothermal activity of an injectable thermosensitive composite hydrogel based on hydroxypropyl chitin (HPCH), tannic acid (TA) and ferric ions (Fe3+) was studied.
34047310	0	53	theme	hydroxypropyl	60:72	arg1	hydrogel					81:88	An injectable photothermally active antibacterial composite hydroxypropyl chitin hydrogel	0:88	An injectable photothermally active antibacterial composite hydroxypropyl chitin hydrogel for promoting the wound healing process through photobiomodulation.	0:156	An injectable photothermally active antibacterial composite hydroxypropyl chitin hydrogel for promoting the wound healing process through photobiomodulation.
34047310	3	54	theme	Fe3+/TA	566:572	arg1	ratio					557:561	the molar ratio	547:561	the molar ratio of Fe3+/TA	547:572	It was found that the photothermal efficiency was enhanced when the molar ratio of Fe3+/TA increased up to 20.
34047310	2	55	theme	thermosensitive	357:371	arg1	hydrogel					383:390	an injectable thermosensitive composite hydrogel	343:390	an injectable thermosensitive composite hydrogel based on hydroxypropyl chitin (HPCH), tannic acid (TA) and ferric ions (Fe3+)	343:468	In this work, the photothermal activity of an injectable thermosensitive composite hydrogel based on hydroxypropyl chitin (HPCH), tannic acid (TA) and ferric ions (Fe3+) was studied.
34047310	7	56	theme	great	1146:1150	arg1	potential					1152:1160	great potential	1146:1160	great potential for the infected skin wound regeneration in clinical applications	1146:1226	Thus, the injectable photothermally active antibacterial composite hydrogel has great potential for the infected skin wound regeneration in clinical applications.
34047310	2	57	theme	injectable	346:355	arg1	hydrogel					383:390	an injectable thermosensitive composite hydrogel	343:390	an injectable thermosensitive composite hydrogel based on hydroxypropyl chitin (HPCH), tannic acid (TA) and ferric ions (Fe3+)	343:468	In this work, the photothermal activity of an injectable thermosensitive composite hydrogel based on hydroxypropyl chitin (HPCH), tannic acid (TA) and ferric ions (Fe3+) was studied.
34047310	6	58	theme	closure	1021:1027	arg1	promotion					1033:1041	promotion	1033:1041	promotion of skin tissue repair	1033:1063	Furthermore, the combination of a low-level laser therapy with the hydrogel is conducive to the acceleration of wound closure and promotion of skin tissue repair.
34047310	6	58	theme	closure	1021:1027	arg1	acceleration					999:1010	the acceleration	995:1010	the acceleration of wound closure	995:1027	Furthermore, the combination of a low-level laser therapy with the hydrogel is conducive to the acceleration of wound closure and promotion of skin tissue repair.
34047310	1	59	from	crucial	262:268	arg1	process					291:297	the wound healing process	273:297	the wound healing process	273:297	Prevention of bacterial infection, acceleration of wound closure and promotion of skin regeneration are crucial in the wound healing process.
34047310	4	60	theme	agent	711:715	arg1	dosage					683:688	a low dosage	677:688	a low dosage of the antibacterial agent TA	677:718	The composite hydrogel possessed good cytocompatibility and hemocompatibility with a low dosage of the antibacterial agent TA.
34047310	6	61	theme	wound	1015:1019	arg1	closure					1021:1027	wound closure	1015:1027	wound closure	1015:1027	Furthermore, the combination of a low-level laser therapy with the hydrogel is conducive to the acceleration of wound closure and promotion of skin tissue repair.
34047310	5	62	theme	in	734:735	arg1	tests					756:760	In vitro and in vivo antibacterial tests	721:760	In vitro and in vivo antibacterial tests	721:760	In vitro and in vivo antibacterial tests showed that the HPCH/TA/Fe hydrogel possessed an effective and rapid bactericidal effect with 10 minutes of near-infrared laser irradiation.
34047310	1	63	theme	bacterial	172:180	arg1	infection					182:190	bacterial infection	172:190	bacterial infection	172:190	Prevention of bacterial infection, acceleration of wound closure and promotion of skin regeneration are crucial in the wound healing process.
34047310	7	64	theme	infected	1170:1177	arg1	regeneration					1190:1201	the infected skin wound regeneration	1166:1201	the infected skin wound regeneration	1166:1201	Thus, the injectable photothermally active antibacterial composite hydrogel has great potential for the infected skin wound regeneration in clinical applications.
34047310	1	65	theme	infection	182:190	arg1	Prevention					158:167	Prevention	158:167	Prevention of bacterial infection	158:190	Prevention of bacterial infection, acceleration of wound closure and promotion of skin regeneration are crucial in the wound healing process.
34047310	1	65	theme	infection	182:190	arg1	promotion					227:235	promotion	227:235	promotion of skin regeneration	227:256	Prevention of bacterial infection, acceleration of wound closure and promotion of skin regeneration are crucial in the wound healing process.
34047310	1	65	theme	infection	182:190	arg1	acceleration					193:204	acceleration	193:204	acceleration of wound closure	193:221	Prevention of bacterial infection, acceleration of wound closure and promotion of skin regeneration are crucial in the wound healing process.
34047310	6	66	theme	low-level	937:945	arg1	therapy					953:959	a low-level laser therapy	935:959	a low-level laser therapy	935:959	Furthermore, the combination of a low-level laser therapy with the hydrogel is conducive to the acceleration of wound closure and promotion of skin tissue repair.
34047310	5	67	theme	In	721:722	arg1	tests					756:760	In vitro and in vivo antibacterial tests	721:760	In vitro and in vivo antibacterial tests	721:760	In vitro and in vivo antibacterial tests showed that the HPCH/TA/Fe hydrogel possessed an effective and rapid bactericidal effect with 10 minutes of near-infrared laser irradiation.
34782210	5	0	theme	prebiotic	709:717	arg1	mixtures					719:726	commercially available prebiotic mixtures	686:726	commercially available prebiotic mixtures using microcrystalline cellulose stationary phases	686:777	Such preparations were prepared by chromatographic fractionation of commercially available prebiotic mixtures using microcrystalline cellulose stationary phases and aqueous ethanol mobile phases.
34782210	7	1	theme	oligosaccharides	1053:1068	arg1	classes					1042:1048	the different classes	1028:1048	the different classes of oligosaccharides	1028:1068	Methods for the characterization of the different classes of oligosaccharides are also presented including those addressing purity, identity, total carbohydrate content, moles per unit mass, and DP.
34782210	1	2	theme	due	183:185	arg1	industry					174:181	the food industry	165:181	the food industry due to their potential health benefits	165:220	Prebiotic oligosaccharides are of widespread interest in the food industry due to their potential health benefits.
34782210	0	3	theme	polymerization	92:105	arg1	degree					82:87	defined degree	74:87	defined degree of polymerization	74:105	Chromatographic preparation of food-grade prebiotic oligosaccharides with defined degree of polymerization.
34782210	6	4	theme	DP	984:985	arg1	galactooligosaccharides					917:939	galactooligosaccharides	917:939	galactooligosaccharides of DP 3 and 4	917:953	This approach is shown to work for the preparation of food-grade fructooligosaccharides of DP 3 and 4, galactooligosaccharides of DP 3 and 4, and xylooligosaccharides of DP 2-4.
34782210	6	4	theme	DP	984:985	arg1	xylooligosaccharides					960:979	xylooligosaccharides	960:979	xylooligosaccharides of DP 2-4	960:989	This approach is shown to work for the preparation of food-grade fructooligosaccharides of DP 3 and 4, galactooligosaccharides of DP 3 and 4, and xylooligosaccharides of DP 2-4.
34782210	6	4	theme	DP	984:985	arg1	fructooligosaccharides					879:900	food-grade fructooligosaccharides	868:900	food-grade fructooligosaccharides of DP 3 and 4	868:914	This approach is shown to work for the preparation of food-grade fructooligosaccharides of DP 3 and 4, galactooligosaccharides of DP 3 and 4, and xylooligosaccharides of DP 2-4.
34782210	5	5	theme	mixtures	719:726	arg1	fractionation					669:681	chromatographic fractionation	653:681	chromatographic fractionation of commercially available prebiotic mixtures using microcrystalline cellulose stationary phases	653:777	Such preparations were prepared by chromatographic fractionation of commercially available prebiotic mixtures using microcrystalline cellulose stationary phases and aqueous ethanol mobile phases.
34782210	5	5	theme	mixtures	719:726	arg1	phases					806:811	aqueous ethanol mobile phases	783:811	aqueous ethanol mobile phases	783:811	Such preparations were prepared by chromatographic fractionation of commercially available prebiotic mixtures using microcrystalline cellulose stationary phases and aqueous ethanol mobile phases.
34782210	7	6	theme	different	1032:1040	arg1	classes					1042:1048	the different classes	1028:1048	the different classes of oligosaccharides	1028:1068	Methods for the characterization of the different classes of oligosaccharides are also presented including those addressing purity, identity, total carbohydrate content, moles per unit mass, and DP.
34782210	3	7	theme	preparations	384:395	arg1	lack					339:342	the lack	335:342	the lack of available food-grade oligosaccharide preparations with specific degree of polymerization (DP)	335:439	Such research is currently limited due to the lack of available food-grade oligosaccharide preparations with specific degree of polymerization (DP).
34782210	6	8	theme	DP	905:906	arg1	galactooligosaccharides					917:939	galactooligosaccharides	917:939	galactooligosaccharides of DP 3 and 4	917:953	This approach is shown to work for the preparation of food-grade fructooligosaccharides of DP 3 and 4, galactooligosaccharides of DP 3 and 4, and xylooligosaccharides of DP 2-4.
34782210	6	8	theme	DP	905:906	arg1	xylooligosaccharides					960:979	xylooligosaccharides	960:979	xylooligosaccharides of DP 2-4	960:989	This approach is shown to work for the preparation of food-grade fructooligosaccharides of DP 3 and 4, galactooligosaccharides of DP 3 and 4, and xylooligosaccharides of DP 2-4.
34782210	6	8	theme	DP	905:906	arg1	fructooligosaccharides					879:900	food-grade fructooligosaccharides	868:900	food-grade fructooligosaccharides of DP 3 and 4	868:914	This approach is shown to work for the preparation of food-grade fructooligosaccharides of DP 3 and 4, galactooligosaccharides of DP 3 and 4, and xylooligosaccharides of DP 2-4.
34782210	6	9	theme	xylooligosaccharides	960:979	arg1	preparation					853:863	the preparation	849:863	the preparation of food-grade fructooligosaccharides of DP 3 and 4, galactooligosaccharides of DP 3 and 4, and xylooligosaccharides of DP 2-4	849:989	This approach is shown to work for the preparation of food-grade fructooligosaccharides of DP 3 and 4, galactooligosaccharides of DP 3 and 4, and xylooligosaccharides of DP 2-4.
34782210	5	10	theme	microcrystalline	734:749	arg1	cellulose					751:759	microcrystalline cellulose	734:759	microcrystalline cellulose stationary phases	734:777	Such preparations were prepared by chromatographic fractionation of commercially available prebiotic mixtures using microcrystalline cellulose stationary phases and aqueous ethanol mobile phases.
34782210	3	11	theme	food-grade	357:366	arg1	preparations					384:395	available food-grade oligosaccharide preparations	347:395	available food-grade oligosaccharide preparations with specific degree of polymerization (DP)	347:439	Such research is currently limited due to the lack of available food-grade oligosaccharide preparations with specific degree of polymerization (DP).
34782210	4	12	theme	economical	479:488	arg1	approaches					490:499	economical approaches	479:499	economical approaches for the preparation and characterization of prebiotic oligosaccharides differing with respect to composition and DP	479:615	The aim of this study was to develop economical approaches for the preparation and characterization of prebiotic oligosaccharides differing with respect to composition and DP.
34782210	7	13	theme	unit	1172:1175	arg1	mass					1177:1180	unit mass	1172:1180	unit mass	1172:1180	Methods for the characterization of the different classes of oligosaccharides are also presented including those addressing purity, identity, total carbohydrate content, moles per unit mass, and DP.
34782210	5	14	theme	chromatographic	653:667	arg1	fractionation					669:681	chromatographic fractionation	653:681	chromatographic fractionation of commercially available prebiotic mixtures using microcrystalline cellulose stationary phases	653:777	Such preparations were prepared by chromatographic fractionation of commercially available prebiotic mixtures using microcrystalline cellulose stationary phases and aqueous ethanol mobile phases.
34782210	3	15	theme	oligosaccharide	368:382	arg1	preparations					384:395	available food-grade oligosaccharide preparations	347:395	available food-grade oligosaccharide preparations with specific degree of polymerization (DP)	347:439	Such research is currently limited due to the lack of available food-grade oligosaccharide preparations with specific degree of polymerization (DP).
34782210	6	16	theme	food-grade	868:877	arg1	fructooligosaccharides					879:900	food-grade fructooligosaccharides	868:900	food-grade fructooligosaccharides of DP 3 and 4	868:914	This approach is shown to work for the preparation of food-grade fructooligosaccharides of DP 3 and 4, galactooligosaccharides of DP 3 and 4, and xylooligosaccharides of DP 2-4.
34782210	3	17	theme	polymerization	421:434	arg1	degree					411:416	specific degree	402:416	specific degree of polymerization (DP)	402:439	Such research is currently limited due to the lack of available food-grade oligosaccharide preparations with specific degree of polymerization (DP).
34782210	1	18	theme	potential	196:204	arg1	benefits					213:220	their potential health benefits	190:220	their potential health benefits	190:220	Prebiotic oligosaccharides are of widespread interest in the food industry due to their potential health benefits.
34782210	1	19	theme	Prebiotic	108:116	arg1	oligosaccharides					118:133	Prebiotic oligosaccharides	108:133	Prebiotic oligosaccharides	108:133	Prebiotic oligosaccharides are of widespread interest in the food industry due to their potential health benefits.
34782210	1	20	theme	health	206:211	arg1	benefits					213:220	their potential health benefits	190:220	their potential health benefits	190:220	Prebiotic oligosaccharides are of widespread interest in the food industry due to their potential health benefits.
34782210	0	21	theme	Chromatographic	0:14	arg1	preparation					16:26	Chromatographic preparation	0:26	Chromatographic preparation of food-grade prebiotic	0:50	Chromatographic preparation of food-grade prebiotic oligosaccharides with defined degree of polymerization.
34782210	5	22	theme	available	699:707	arg1	mixtures					719:726	commercially available prebiotic mixtures	686:726	commercially available prebiotic mixtures using microcrystalline cellulose stationary phases	686:777	Such preparations were prepared by chromatographic fractionation of commercially available prebiotic mixtures using microcrystalline cellulose stationary phases and aqueous ethanol mobile phases.
34782210	5	23	theme	Such	618:621	arg1	preparations					623:634	Such preparations	618:634	Such preparations	618:634	Such preparations were prepared by chromatographic fractionation of commercially available prebiotic mixtures using microcrystalline cellulose stationary phases and aqueous ethanol mobile phases.
34782210	3	24	theme	specific	402:409	arg1	degree					411:416	specific degree	402:416	specific degree of polymerization (DP)	402:439	Such research is currently limited due to the lack of available food-grade oligosaccharide preparations with specific degree of polymerization (DP).
34782210	5	25	theme	aqueous	783:789	arg1	phases					806:811	aqueous ethanol mobile phases	783:811	aqueous ethanol mobile phases	783:811	Such preparations were prepared by chromatographic fractionation of commercially available prebiotic mixtures using microcrystalline cellulose stationary phases and aqueous ethanol mobile phases.
34782210	1	26	from	interest	153:160	arg1	industry					174:181	the food industry	165:181	the food industry due to their potential health benefits	165:220	Prebiotic oligosaccharides are of widespread interest in the food industry due to their potential health benefits.
34782210	7	27	theme	total	1134:1138	arg1	content					1153:1159	total carbohydrate content	1134:1159	total carbohydrate content	1134:1159	Methods for the characterization of the different classes of oligosaccharides are also presented including those addressing purity, identity, total carbohydrate content, moles per unit mass, and DP.
34782210	5	28	theme	ethanol	791:797	arg1	phases					806:811	aqueous ethanol mobile phases	783:811	aqueous ethanol mobile phases	783:811	Such preparations were prepared by chromatographic fractionation of commercially available prebiotic mixtures using microcrystalline cellulose stationary phases and aqueous ethanol mobile phases.
34782210	5	29	theme	mobile	799:804	arg1	phases					806:811	aqueous ethanol mobile phases	783:811	aqueous ethanol mobile phases	783:811	Such preparations were prepared by chromatographic fractionation of commercially available prebiotic mixtures using microcrystalline cellulose stationary phases and aqueous ethanol mobile phases.
34782210	4	30	theme	study	458:462	arg1	aim					446:448	The aim	442:448	The aim of this study	442:462	The aim of this study was to develop economical approaches for the preparation and characterization of prebiotic oligosaccharides differing with respect to composition and DP.
34782210	0	31	theme	prebiotic	42:50	arg1	preparation					16:26	Chromatographic preparation	0:26	Chromatographic preparation of food-grade prebiotic	0:50	Chromatographic preparation of food-grade prebiotic oligosaccharides with defined degree of polymerization.
34782210	4	32	theme	oligosaccharides	555:570	arg1	characterization					525:540	characterization	525:540	characterization	525:540	The aim of this study was to develop economical approaches for the preparation and characterization of prebiotic oligosaccharides differing with respect to composition and DP.
34782210	4	32	theme	oligosaccharides	555:570	arg1	preparation					509:519	preparation	509:519	preparation	509:519	The aim of this study was to develop economical approaches for the preparation and characterization of prebiotic oligosaccharides differing with respect to composition and DP.
34782210	3	33	theme	Such	293:296	arg1	research					298:305	Such research	293:305	Such research	293:305	Such research is currently limited due to the lack of available food-grade oligosaccharide preparations with specific degree of polymerization (DP).
34782210	6	34	theme	DP	944:945	arg1	galactooligosaccharides					917:939	galactooligosaccharides	917:939	galactooligosaccharides of DP 3 and 4	917:953	This approach is shown to work for the preparation of food-grade fructooligosaccharides of DP 3 and 4, galactooligosaccharides of DP 3 and 4, and xylooligosaccharides of DP 2-4.
34782210	6	34	theme	DP	944:945	arg1	xylooligosaccharides					960:979	xylooligosaccharides	960:979	xylooligosaccharides of DP 2-4	960:989	This approach is shown to work for the preparation of food-grade fructooligosaccharides of DP 3 and 4, galactooligosaccharides of DP 3 and 4, and xylooligosaccharides of DP 2-4.
34782210	6	34	theme	DP	944:945	arg1	fructooligosaccharides					879:900	food-grade fructooligosaccharides	868:900	food-grade fructooligosaccharides of DP 3 and 4	868:914	This approach is shown to work for the preparation of food-grade fructooligosaccharides of DP 3 and 4, galactooligosaccharides of DP 3 and 4, and xylooligosaccharides of DP 2-4.
34782210	0	35	theme	food-grade	31:40	arg1	prebiotic					42:50	food-grade prebiotic	31:50	food-grade prebiotic	31:50	Chromatographic preparation of food-grade prebiotic oligosaccharides with defined degree of polymerization.
34782210	0	36	theme	defined	74:80	arg1	degree					82:87	defined degree	74:87	defined degree of polymerization	74:105	Chromatographic preparation of food-grade prebiotic oligosaccharides with defined degree of polymerization.
34782210	4	37	theme	prebiotic	545:553	arg1	oligosaccharides					555:570	prebiotic oligosaccharides	545:570	prebiotic oligosaccharides differing with respect to composition and DP	545:615	The aim of this study was to develop economical approaches for the preparation and characterization of prebiotic oligosaccharides differing with respect to composition and DP.
34782210	5	38	theme	cellulose	751:759	arg1	phases					772:777	microcrystalline cellulose stationary phases	734:777	microcrystalline cellulose stationary phases	734:777	Such preparations were prepared by chromatographic fractionation of commercially available prebiotic mixtures using microcrystalline cellulose stationary phases and aqueous ethanol mobile phases.
34782210	3	39	with	preparations	384:395	arg1	degree					411:416	specific degree	402:416	specific degree of polymerization (DP)	402:439	Such research is currently limited due to the lack of available food-grade oligosaccharide preparations with specific degree of polymerization (DP).
34782210	1	40	theme	widespread	142:151	arg1	interest					153:160	widespread interest	142:160	widespread interest	142:160	Prebiotic oligosaccharides are of widespread interest in the food industry due to their potential health benefits.
34782210	7	41	theme	carbohydrate	1140:1151	arg1	content					1153:1159	total carbohydrate content	1134:1159	total carbohydrate content	1134:1159	Methods for the characterization of the different classes of oligosaccharides are also presented including those addressing purity, identity, total carbohydrate content, moles per unit mass, and DP.
34782210	5	42	theme	stationary	761:770	arg1	phases					772:777	microcrystalline cellulose stationary phases	734:777	microcrystalline cellulose stationary phases	734:777	Such preparations were prepared by chromatographic fractionation of commercially available prebiotic mixtures using microcrystalline cellulose stationary phases and aqueous ethanol mobile phases.
34782210	6	43	theme	galactooligosaccharides	917:939	arg1	preparation					853:863	the preparation	849:863	the preparation of food-grade fructooligosaccharides of DP 3 and 4, galactooligosaccharides of DP 3 and 4, and xylooligosaccharides of DP 2-4	849:989	This approach is shown to work for the preparation of food-grade fructooligosaccharides of DP 3 and 4, galactooligosaccharides of DP 3 and 4, and xylooligosaccharides of DP 2-4.
34782210	4	44	dep	preparation	509:519	arg1	the					505:507	the	505:507	the	505:507	The aim of this study was to develop economical approaches for the preparation and characterization of prebiotic oligosaccharides differing with respect to composition and DP.
34782210	7	45	theme	classes	1042:1048	arg1	characterization					1008:1023	the characterization	1004:1023	the characterization of the different classes of oligosaccharides	1004:1068	Methods for the characterization of the different classes of oligosaccharides are also presented including those addressing purity, identity, total carbohydrate content, moles per unit mass, and DP.
34782210	2	46	theme	sensory	273:279	arg1	properties					281:290	their sensory properties	267:290	their sensory properties	267:290	This has triggered a need for research into their sensory properties.
34782210	6	47	theme	fructooligosaccharides	879:900	arg1	preparation					853:863	the preparation	849:863	the preparation of food-grade fructooligosaccharides of DP 3 and 4, galactooligosaccharides of DP 3 and 4, and xylooligosaccharides of DP 2-4	849:989	This approach is shown to work for the preparation of food-grade fructooligosaccharides of DP 3 and 4, galactooligosaccharides of DP 3 and 4, and xylooligosaccharides of DP 2-4.
34782210	1	48	theme	food	169:172	arg1	industry					174:181	the food industry	165:181	the food industry due to their potential health benefits	165:220	Prebiotic oligosaccharides are of widespread interest in the food industry due to their potential health benefits.
34782210	3	49	theme	available	347:355	arg1	preparations					384:395	available food-grade oligosaccharide preparations	347:395	available food-grade oligosaccharide preparations with specific degree of polymerization (DP)	347:439	Such research is currently limited due to the lack of available food-grade oligosaccharide preparations with specific degree of polymerization (DP).
34743417	0	0	theme	clove	92:96	arg1	beads					102:106	chitosan/carrageenan@clove oil beads	71:106	chitosan/carrageenan@clove oil beads	71:106	Antimicrobial and antiviral activities with molecular docking study of chitosan/carrageenan@clove oil beads.
34743417	6	1	theme	clove	924:928	arg1	beads					934:938	chitosan/carrageenan@clove oil beads	903:938	chitosan/carrageenan@clove oil beads	903:938	Antibacterial results revealed that chitosan/carrageenan@clove oil beads have antimicrobial activity as well as chitosan/carrageenan without clove oil against Escherichia coli AATCC25922, Pseudomonas aeruginosa AATCC27853, Staphylococcus aureus ATCC25923, Bacillus subtilis AATCC6051, and Candida albicans ATCC90028.
34743417	9	2	theme	engineering	1773:1783	arg1	carriers					1739:1746	carriers	1739:1746	carriers of drug delivery, tissue engineering, and regenerative medicine	1739:1810	CONCLUSIONS The developed chitosan/carrageenan@clove oil beads have shown promising properties to be used as carriers of drug delivery, tissue engineering, and regenerative medicine.
34743417	2	3	theme	chitosan	364:371	arg1	chitosan					364:371	chitosan	364:371	chitosan	364:371	A promising composite of chitosan and carrageenan with self-crosslinking has been prepared.
34743417	2	3	theme	chitosan	364:371	arg1	composite					351:359	A promising composite	339:359	A promising composite of chitosan and carrageenan with self-crosslinking	339:410	A promising composite of chitosan and carrageenan with self-crosslinking has been prepared.
34743417	2	3	theme	chitosan	364:371	arg1	carrageenan					377:387	carrageenan	377:387	carrageenan	377:387	A promising composite of chitosan and carrageenan with self-crosslinking has been prepared.
34743417	0	4	theme	@	91:91	arg1	beads					102:106	chitosan/carrageenan@clove oil beads	71:106	chitosan/carrageenan@clove oil beads	71:106	Antimicrobial and antiviral activities with molecular docking study of chitosan/carrageenan@clove oil beads.
34743417	3	5	from	METHODS	431:437	arg1	study					459:463	this study	454:463	this study	454:463	METHODS AND RESULTS In this study, a rigorous approach for an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared.
34743417	9	6	theme	regenerative	1790:1801	arg1	medicine					1803:1810	regenerative medicine	1790:1810	regenerative medicine	1790:1810	CONCLUSIONS The developed chitosan/carrageenan@clove oil beads have shown promising properties to be used as carriers of drug delivery, tissue engineering, and regenerative medicine.
34743417	0	7	theme	beads	102:106	arg1	study					62:66	molecular docking study	44:66	molecular docking study of chitosan/carrageenan@clove oil beads	44:106	Antimicrobial and antiviral activities with molecular docking study of chitosan/carrageenan@clove oil beads.
34743417	6	8	contain	have	940:943	arg2	chitosan/carrageenan					979:998	chitosan/carrageenan	979:998	antimicrobial activity as well as chitosan/carrageenan	945:998	Antibacterial results revealed that chitosan/carrageenan@clove oil beads have antimicrobial activity as well as chitosan/carrageenan without clove oil against Escherichia coli AATCC25922, Pseudomonas aeruginosa AATCC27853, Staphylococcus aureus ATCC25923, Bacillus subtilis AATCC6051, and Candida albicans ATCC90028.
34743417	6	8	contain	have	940:943	arg1	beads					934:938	chitosan/carrageenan@clove oil beads	903:938	chitosan/carrageenan@clove oil beads	903:938	Antibacterial results revealed that chitosan/carrageenan@clove oil beads have antimicrobial activity as well as chitosan/carrageenan without clove oil against Escherichia coli AATCC25922, Pseudomonas aeruginosa AATCC27853, Staphylococcus aureus ATCC25923, Bacillus subtilis AATCC6051, and Candida albicans ATCC90028.
34743417	6	8	contain	have	940:943	arg2	activity					959:966	antimicrobial activity	945:966	antimicrobial activity as well as chitosan/carrageenan	945:998	Antibacterial results revealed that chitosan/carrageenan@clove oil beads have antimicrobial activity as well as chitosan/carrageenan without clove oil against Escherichia coli AATCC25922, Pseudomonas aeruginosa AATCC27853, Staphylococcus aureus ATCC25923, Bacillus subtilis AATCC6051, and Candida albicans ATCC90028.
34743417	3	9	theme	clove	555:559	arg1	oil					561:563	clove oil	555:563	clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared	555:663	METHODS AND RESULTS In this study, a rigorous approach for an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared.
34743417	4	10	theme	X-ray	750:754	arg1	XRD					769:771	XRD	769:771	XRD	769:771	The structure and the surface morphology of the beads were investigated using FTIR, X-ray diffraction (XRD), and SEM.
34743417	4	10	theme	X-ray	750:754	arg1	diffraction					756:766	X-ray diffraction	750:766	X-ray diffraction (XRD)	750:772	The structure and the surface morphology of the beads were investigated using FTIR, X-ray diffraction (XRD), and SEM.
34743417	6	11	theme	clove	1008:1012	arg1	oil					1014:1016	clove oil	1008:1016	clove oil against Escherichia coli AATCC25922, Pseudomonas aeruginosa AATCC27853, Staphylococcus aureus ATCC25923, Bacillus subtilis AATCC6051, and Candida albicans ATCC90028	1008:1181	Antibacterial results revealed that chitosan/carrageenan@clove oil beads have antimicrobial activity as well as chitosan/carrageenan without clove oil against Escherichia coli AATCC25922, Pseudomonas aeruginosa AATCC27853, Staphylococcus aureus ATCC25923, Bacillus subtilis AATCC6051, and Candida albicans ATCC90028.
34743417	0	12	theme	oil	98:100	arg1	beads					102:106	chitosan/carrageenan@clove oil beads	71:106	chitosan/carrageenan@clove oil beads	71:106	Antimicrobial and antiviral activities with molecular docking study of chitosan/carrageenan@clove oil beads.
34743417	9	13	theme	promising	1704:1712	arg1	properties					1714:1723	promising properties	1704:1723	promising properties	1704:1723	CONCLUSIONS The developed chitosan/carrageenan@clove oil beads have shown promising properties to be used as carriers of drug delivery, tissue engineering, and regenerative medicine.
34743417	3	14	theme	oil	561:563	arg1	amounts					544:550	different amounts	534:550	different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared	534:663	METHODS AND RESULTS In this study, a rigorous approach for an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared.
34743417	3	14	theme	oil	561:563	arg1	oil					561:563	clove oil	555:563	clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared	555:663	METHODS AND RESULTS In this study, a rigorous approach for an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared.
34743417	9	15	theme	medicine	1803:1810	arg1	carriers					1739:1746	carriers	1739:1746	carriers of drug delivery, tissue engineering, and regenerative medicine	1739:1810	CONCLUSIONS The developed chitosan/carrageenan@clove oil beads have shown promising properties to be used as carriers of drug delivery, tissue engineering, and regenerative medicine.
34743417	7	16	theme	promising	1313:1321	arg1	activity					1333:1340	promising antiviral activity	1313:1340	promising antiviral activity against Herpes simplex virus-1 (HSV-1)	1313:1379	Furthermore, maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads was (31.25 μg ml-1 ) which exhibited promising antiviral activity against Herpes simplex virus-1 (HSV-1), and was significantly higher than chitosan/carrageenan without clove oil, where antiviral activity was 82.94% and 57.64%, respectively.
34743417	3	17	theme	amounts	544:550	arg1	combination					519:529	an inexpensive and non-toxic combination	490:529	an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared	490:663	METHODS AND RESULTS In this study, a rigorous approach for an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared.
34743417	3	18	dep	chitosan	601:608	arg1	prepared					656:663	prepared	656:663	has been prepared	647:663	METHODS AND RESULTS In this study, a rigorous approach for an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared.
34743417	3	19	with	oil	561:563	arg1	carrageenan					614:624	carrageenan	614:624	carrageenan	614:624	METHODS AND RESULTS In this study, a rigorous approach for an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared.
34743417	3	19	with	oil	561:563	arg1	chitosan					601:608	chitosan	601:608	chitosan	601:608	METHODS AND RESULTS In this study, a rigorous approach for an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared.
34743417	3	19	with	oil	561:563	arg1	polyelectrolytes					574:589	two polyelectrolytes	570:589	two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared	570:663	METHODS AND RESULTS In this study, a rigorous approach for an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared.
34743417	8	20	used	used	1584:1587	arg2	calculation					1562:1572	computational calculation	1548:1572	computational calculation	1548:1572	Eventually, docking study and computational calculation have been used to show the reactivity of the molecules.
34743417	8	20	used	used	1584:1587	arg2	study					1538:1542	docking study	1530:1542	docking study	1530:1542	Eventually, docking study and computational calculation have been used to show the reactivity of the molecules.
34743417	7	21	theme	chitosan/carrageenan	1239:1258	arg1	beads					1270:1274	chitosan/carrageenan@clove oil beads	1239:1274	chitosan/carrageenan@clove oil beads	1239:1274	Furthermore, maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads was (31.25 μg ml-1 ) which exhibited promising antiviral activity against Herpes simplex virus-1 (HSV-1), and was significantly higher than chitosan/carrageenan without clove oil, where antiviral activity was 82.94% and 57.64%, respectively.
34743417	1	22	theme	hydrophilic	185:195	arg1	matrices					197:204	hydrophilic matrices	185:204	hydrophilic matrices	185:204	BACKGROUND Biopolymers are promising candidates that can be fabricated into hydrophilic matrices and used for many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost.
34743417	6	23	theme	Antibacterial	867:879	arg1	results					881:887	Antibacterial results	867:887	Antibacterial results	867:887	Antibacterial results revealed that chitosan/carrageenan@clove oil beads have antimicrobial activity as well as chitosan/carrageenan without clove oil against Escherichia coli AATCC25922, Pseudomonas aeruginosa AATCC27853, Staphylococcus aureus ATCC25923, Bacillus subtilis AATCC6051, and Candida albicans ATCC90028.
34743417	7	24	theme	oil	1266:1268	arg1	beads					1270:1274	chitosan/carrageenan@clove oil beads	1239:1274	chitosan/carrageenan@clove oil beads	1239:1274	Furthermore, maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads was (31.25 μg ml-1 ) which exhibited promising antiviral activity against Herpes simplex virus-1 (HSV-1), and was significantly higher than chitosan/carrageenan without clove oil, where antiviral activity was 82.94% and 57.64%, respectively.
34743417	7	25	theme	beads	1270:1274	arg1	concentration					1215:1227	maximum non-toxic concentration	1197:1227	maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads	1197:1274	Furthermore, maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads was (31.25 μg ml-1 ) which exhibited promising antiviral activity against Herpes simplex virus-1 (HSV-1), and was significantly higher than chitosan/carrageenan without clove oil, where antiviral activity was 82.94% and 57.64%, respectively.
34743417	7	25	theme	beads	1270:1274	arg1	MNTC					1230:1233	MNTC	1230:1233	MNTC	1230:1233	Furthermore, maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads was (31.25 μg ml-1 ) which exhibited promising antiviral activity against Herpes simplex virus-1 (HSV-1), and was significantly higher than chitosan/carrageenan without clove oil, where antiviral activity was 82.94% and 57.64%, respectively.
34743417	8	26	theme	computational	1548:1560	arg1	calculation					1562:1572	computational calculation	1548:1572	computational calculation	1548:1572	Eventually, docking study and computational calculation have been used to show the reactivity of the molecules.
34743417	6	27	theme	@	923:923	arg1	beads					934:938	chitosan/carrageenan@clove oil beads	903:938	chitosan/carrageenan@clove oil beads	903:938	Antibacterial results revealed that chitosan/carrageenan@clove oil beads have antimicrobial activity as well as chitosan/carrageenan without clove oil against Escherichia coli AATCC25922, Pseudomonas aeruginosa AATCC27853, Staphylococcus aureus ATCC25923, Bacillus subtilis AATCC6051, and Candida albicans ATCC90028.
34743417	9	28	theme	drug	1751:1754	arg1	delivery					1756:1763	drug delivery	1751:1763	drug delivery	1751:1763	CONCLUSIONS The developed chitosan/carrageenan@clove oil beads have shown promising properties to be used as carriers of drug delivery, tissue engineering, and regenerative medicine.
34743417	9	29	theme	@	1676:1676	arg1	beads					1687:1691	The developed chitosan/carrageenan@clove oil beads	1642:1691	CONCLUSIONS The developed chitosan/carrageenan@clove oil beads	1630:1691	CONCLUSIONS The developed chitosan/carrageenan@clove oil beads have shown promising properties to be used as carriers of drug delivery, tissue engineering, and regenerative medicine.
34743417	9	30	dep	CONCLUSIONS	1630:1640	arg1	beads					1687:1691	The developed chitosan/carrageenan@clove oil beads	1642:1691	CONCLUSIONS The developed chitosan/carrageenan@clove oil beads	1630:1691	CONCLUSIONS The developed chitosan/carrageenan@clove oil beads have shown promising properties to be used as carriers of drug delivery, tissue engineering, and regenerative medicine.
34743417	7	31	theme	non-toxic	1205:1213	arg1	concentration					1215:1227	maximum non-toxic concentration	1197:1227	maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads	1197:1274	Furthermore, maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads was (31.25 μg ml-1 ) which exhibited promising antiviral activity against Herpes simplex virus-1 (HSV-1), and was significantly higher than chitosan/carrageenan without clove oil, where antiviral activity was 82.94% and 57.64%, respectively.
34743417	7	31	theme	non-toxic	1205:1213	arg1	MNTC					1230:1233	MNTC	1230:1233	MNTC	1230:1233	Furthermore, maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads was (31.25 μg ml-1 ) which exhibited promising antiviral activity against Herpes simplex virus-1 (HSV-1), and was significantly higher than chitosan/carrageenan without clove oil, where antiviral activity was 82.94% and 57.64%, respectively.
34743417	9	32	theme	clove	1677:1681	arg1	beads					1687:1691	The developed chitosan/carrageenan@clove oil beads	1642:1691	CONCLUSIONS The developed chitosan/carrageenan@clove oil beads	1630:1691	CONCLUSIONS The developed chitosan/carrageenan@clove oil beads have shown promising properties to be used as carriers of drug delivery, tissue engineering, and regenerative medicine.
34743417	9	33	theme	developed	1646:1654	arg1	beads					1687:1691	The developed chitosan/carrageenan@clove oil beads	1642:1691	CONCLUSIONS The developed chitosan/carrageenan@clove oil beads	1630:1691	CONCLUSIONS The developed chitosan/carrageenan@clove oil beads have shown promising properties to be used as carriers of drug delivery, tissue engineering, and regenerative medicine.
34743417	7	34	theme	clove	1445:1449	arg1	oil					1451:1453	clove oil	1445:1453	clove oil	1445:1453	Furthermore, maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads was (31.25 μg ml-1 ) which exhibited promising antiviral activity against Herpes simplex virus-1 (HSV-1), and was significantly higher than chitosan/carrageenan without clove oil, where antiviral activity was 82.94% and 57.64%, respectively.
34743417	3	35	theme	rigorous	468:475	arg1	METHODS					431:437	METHODS	431:437	METHODS	431:437	METHODS AND RESULTS In this study, a rigorous approach for an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared.
34743417	3	35	theme	rigorous	468:475	arg1	approach					477:484	a rigorous approach	466:484	a rigorous approach for an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared	466:663	METHODS AND RESULTS In this study, a rigorous approach for an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared.
34743417	0	36	theme	antiviral	18:26	arg1	activities					28:37	antiviral activities	18:37	antiviral activities	18:37	Antimicrobial and antiviral activities with molecular docking study of chitosan/carrageenan@clove oil beads.
34743417	7	37	theme	antiviral	1462:1470	arg1	activity					1472:1479	antiviral activity	1462:1479	antiviral activity	1462:1479	Furthermore, maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads was (31.25 μg ml-1 ) which exhibited promising antiviral activity against Herpes simplex virus-1 (HSV-1), and was significantly higher than chitosan/carrageenan without clove oil, where antiviral activity was 82.94% and 57.64%, respectively.
34743417	1	38	theme	BACKGROUND	109:118	arg1	Biopolymers					120:130	BACKGROUND Biopolymers	109:130	BACKGROUND Biopolymers	109:130	BACKGROUND Biopolymers are promising candidates that can be fabricated into hydrophilic matrices and used for many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost.
34743417	1	38	theme	BACKGROUND	109:118	arg1	candidates					146:155	promising candidates	136:155	promising candidates that can be fabricated into hydrophilic matrices and used for many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost	136:336	BACKGROUND Biopolymers are promising candidates that can be fabricated into hydrophilic matrices and used for many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost.
34743417	7	39	theme	simplex	1357:1363	arg1	HSV-1					1374:1378	HSV-1	1374:1378	HSV-1	1374:1378	Furthermore, maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads was (31.25 μg ml-1 ) which exhibited promising antiviral activity against Herpes simplex virus-1 (HSV-1), and was significantly higher than chitosan/carrageenan without clove oil, where antiviral activity was 82.94% and 57.64%, respectively.
34743417	7	39	theme	simplex	1357:1363	arg1	virus-1					1365:1371	Herpes simplex virus-1	1350:1371	Herpes simplex virus-1 (HSV-1)	1350:1379	Furthermore, maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads was (31.25 μg ml-1 ) which exhibited promising antiviral activity against Herpes simplex virus-1 (HSV-1), and was significantly higher than chitosan/carrageenan without clove oil, where antiviral activity was 82.94% and 57.64%, respectively.
34743417	0	40	theme	molecular	44:52	arg1	study					62:66	molecular docking study	44:66	molecular docking study of chitosan/carrageenan@clove oil beads	44:106	Antimicrobial and antiviral activities with molecular docking study of chitosan/carrageenan@clove oil beads.
34743417	4	41	theme	beads	714:718	arg1	structure					670:678	structure	670:678	structure	670:678	The structure and the surface morphology of the beads were investigated using FTIR, X-ray diffraction (XRD), and SEM.
34743417	4	41	theme	beads	714:718	arg1	morphology					696:705	the surface morphology	684:705	the surface morphology	684:705	The structure and the surface morphology of the beads were investigated using FTIR, X-ray diffraction (XRD), and SEM.
34743417	8	42	theme	molecules	1619:1627	arg1	reactivity					1601:1610	the reactivity	1597:1610	the reactivity of the molecules	1597:1627	Eventually, docking study and computational calculation have been used to show the reactivity of the molecules.
34743417	1	43	theme	many	219:222	arg1	applications					224:235	many applications	219:235	many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost	219:336	BACKGROUND Biopolymers are promising candidates that can be fabricated into hydrophilic matrices and used for many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost.
34743417	7	44	theme	maximum	1197:1203	arg1	concentration					1215:1227	maximum non-toxic concentration	1197:1227	maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads	1197:1274	Furthermore, maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads was (31.25 μg ml-1 ) which exhibited promising antiviral activity against Herpes simplex virus-1 (HSV-1), and was significantly higher than chitosan/carrageenan without clove oil, where antiviral activity was 82.94% and 57.64%, respectively.
34743417	7	44	theme	maximum	1197:1203	arg1	MNTC					1230:1233	MNTC	1230:1233	MNTC	1230:1233	Furthermore, maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads was (31.25 μg ml-1 ) which exhibited promising antiviral activity against Herpes simplex virus-1 (HSV-1), and was significantly higher than chitosan/carrageenan without clove oil, where antiviral activity was 82.94% and 57.64%, respectively.
34743417	7	45	dep	was	1276:1278	arg1	ml-1					1290:1293	31.25 μg ml-1	1281:1293	31.25 μg ml-1	1281:1293	Furthermore, maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads was (31.25 μg ml-1 ) which exhibited promising antiviral activity against Herpes simplex virus-1 (HSV-1), and was significantly higher than chitosan/carrageenan without clove oil, where antiviral activity was 82.94% and 57.64%, respectively.
34743417	2	46	with	composite	351:359	arg1	self-crosslinking					394:410	self-crosslinking	394:410	self-crosslinking	394:410	A promising composite of chitosan and carrageenan with self-crosslinking has been prepared.
34743417	0	47	with	activities	28:37	arg1	study					62:66	molecular docking study	44:66	molecular docking study of chitosan/carrageenan@clove oil beads	44:106	Antimicrobial and antiviral activities with molecular docking study of chitosan/carrageenan@clove oil beads.
34743417	5	48	theme	molecular	833:841	arg1	docking					843:849	molecular docking	833:849	molecular docking	833:849	Moreover, antimicrobial, antiviral activity, and molecular docking were evaluated.
34743417	3	49	theme	non-toxic	509:517	arg1	combination					519:529	an inexpensive and non-toxic combination	490:529	an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared	490:663	METHODS AND RESULTS In this study, a rigorous approach for an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared.
34743417	9	50	theme	delivery	1756:1763	arg1	carriers					1739:1746	carriers	1739:1746	carriers of drug delivery, tissue engineering, and regenerative medicine	1739:1810	CONCLUSIONS The developed chitosan/carrageenan@clove oil beads have shown promising properties to be used as carriers of drug delivery, tissue engineering, and regenerative medicine.
34743417	3	51	theme	inexpensive	493:503	arg1	combination					519:529	an inexpensive and non-toxic combination	490:529	an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared	490:663	METHODS AND RESULTS In this study, a rigorous approach for an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared.
34743417	0	52	theme	docking	54:60	arg1	study					62:66	molecular docking study	44:66	molecular docking study of chitosan/carrageenan@clove oil beads	44:106	Antimicrobial and antiviral activities with molecular docking study of chitosan/carrageenan@clove oil beads.
34743417	1	53	theme	low	329:331	arg1	cost					333:336	low cost	329:336	low cost	329:336	BACKGROUND Biopolymers are promising candidates that can be fabricated into hydrophilic matrices and used for many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost.
34743417	8	54	theme	docking	1530:1536	arg1	study					1538:1542	docking study	1530:1542	docking study	1530:1542	Eventually, docking study and computational calculation have been used to show the reactivity of the molecules.
34743417	1	55	used	used	210:213	arg2	Biopolymers					120:130	BACKGROUND Biopolymers	109:130	BACKGROUND Biopolymers	109:130	BACKGROUND Biopolymers are promising candidates that can be fabricated into hydrophilic matrices and used for many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost.
34743417	1	55	used	used	210:213	arg2	candidates					146:155	promising candidates	136:155	promising candidates that can be fabricated into hydrophilic matrices and used for many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost	136:336	BACKGROUND Biopolymers are promising candidates that can be fabricated into hydrophilic matrices and used for many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost.
34743417	7	56	theme	@	1259:1259	arg1	beads					1270:1274	chitosan/carrageenan@clove oil beads	1239:1274	chitosan/carrageenan@clove oil beads	1239:1274	Furthermore, maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads was (31.25 μg ml-1 ) which exhibited promising antiviral activity against Herpes simplex virus-1 (HSV-1), and was significantly higher than chitosan/carrageenan without clove oil, where antiviral activity was 82.94% and 57.64%, respectively.
34743417	2	57	theme	promising	341:349	arg1	chitosan					364:371	chitosan	364:371	chitosan	364:371	A promising composite of chitosan and carrageenan with self-crosslinking has been prepared.
34743417	2	57	theme	promising	341:349	arg1	composite					351:359	A promising composite	339:359	A promising composite of chitosan and carrageenan with self-crosslinking	339:410	A promising composite of chitosan and carrageenan with self-crosslinking has been prepared.
34743417	2	57	theme	promising	341:349	arg1	carrageenan					377:387	carrageenan	377:387	carrageenan	377:387	A promising composite of chitosan and carrageenan with self-crosslinking has been prepared.
34743417	9	58	theme	oil	1683:1685	arg1	beads					1687:1691	The developed chitosan/carrageenan@clove oil beads	1642:1691	CONCLUSIONS The developed chitosan/carrageenan@clove oil beads	1630:1691	CONCLUSIONS The developed chitosan/carrageenan@clove oil beads have shown promising properties to be used as carriers of drug delivery, tissue engineering, and regenerative medicine.
34743417	9	59	theme	tissue	1766:1771	arg1	engineering					1773:1783	tissue engineering	1766:1783	tissue engineering	1766:1783	CONCLUSIONS The developed chitosan/carrageenan@clove oil beads have shown promising properties to be used as carriers of drug delivery, tissue engineering, and regenerative medicine.
34743417	9	60	theme	chitosan/carrageenan	1656:1675	arg1	beads					1687:1691	The developed chitosan/carrageenan@clove oil beads	1642:1691	CONCLUSIONS The developed chitosan/carrageenan@clove oil beads	1630:1691	CONCLUSIONS The developed chitosan/carrageenan@clove oil beads have shown promising properties to be used as carriers of drug delivery, tissue engineering, and regenerative medicine.
34743417	0	61	with	Antimicrobial	0:12	arg1	study					62:66	molecular docking study	44:66	molecular docking study of chitosan/carrageenan@clove oil beads	44:106	Antimicrobial and antiviral activities with molecular docking study of chitosan/carrageenan@clove oil beads.
34743417	1	62	theme	promising	136:144	arg1	Biopolymers					120:130	BACKGROUND Biopolymers	109:130	BACKGROUND Biopolymers	109:130	BACKGROUND Biopolymers are promising candidates that can be fabricated into hydrophilic matrices and used for many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost.
34743417	1	62	theme	promising	136:144	arg1	candidates					146:155	promising candidates	136:155	promising candidates that can be fabricated into hydrophilic matrices and used for many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost	136:336	BACKGROUND Biopolymers are promising candidates that can be fabricated into hydrophilic matrices and used for many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost.
34743417	1	63	theme	due	237:239	arg1	applications					224:235	many applications	219:235	many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost	219:336	BACKGROUND Biopolymers are promising candidates that can be fabricated into hydrophilic matrices and used for many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost.
34743417	7	64	theme	clove	1260:1264	arg1	beads					1270:1274	chitosan/carrageenan@clove oil beads	1239:1274	chitosan/carrageenan@clove oil beads	1239:1274	Furthermore, maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads was (31.25 μg ml-1 ) which exhibited promising antiviral activity against Herpes simplex virus-1 (HSV-1), and was significantly higher than chitosan/carrageenan without clove oil, where antiviral activity was 82.94% and 57.64%, respectively.
34743417	7	65	theme	antiviral	1323:1331	arg1	activity					1333:1340	promising antiviral activity	1313:1340	promising antiviral activity against Herpes simplex virus-1 (HSV-1)	1313:1379	Furthermore, maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads was (31.25 μg ml-1 ) which exhibited promising antiviral activity against Herpes simplex virus-1 (HSV-1), and was significantly higher than chitosan/carrageenan without clove oil, where antiviral activity was 82.94% and 57.64%, respectively.
34743417	3	66	theme	beads	641:645	arg1	form					633:636	the form	629:636	the form of beads	629:645	METHODS AND RESULTS In this study, a rigorous approach for an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared.
34743417	3	67	from	RESULTS	443:449	arg1	study					459:463	this study	454:463	this study	454:463	METHODS AND RESULTS In this study, a rigorous approach for an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared.
34743417	4	68	theme	surface	688:694	arg1	morphology					696:705	the surface morphology	684:705	the surface morphology	684:705	The structure and the surface morphology of the beads were investigated using FTIR, X-ray diffraction (XRD), and SEM.
34743417	6	69	theme	Candida	1156:1162	arg1	albicans					1164:1171	Candida albicans ATCC90028	1156:1181	Candida albicans ATCC90028	1156:1181	Antibacterial results revealed that chitosan/carrageenan@clove oil beads have antimicrobial activity as well as chitosan/carrageenan without clove oil against Escherichia coli AATCC25922, Pseudomonas aeruginosa AATCC27853, Staphylococcus aureus ATCC25923, Bacillus subtilis AATCC6051, and Candida albicans ATCC90028.
34743417	0	70	theme	chitosan/carrageenan	71:90	arg1	beads					102:106	chitosan/carrageenan@clove oil beads	71:106	chitosan/carrageenan@clove oil beads	71:106	Antimicrobial and antiviral activities with molecular docking study of chitosan/carrageenan@clove oil beads.
34743417	4	71	dep	structure	670:678	arg1	The					666:668	The	666:668	The	666:668	The structure and the surface morphology of the beads were investigated using FTIR, X-ray diffraction (XRD), and SEM.
34743417	7	72	theme	Herpes	1350:1355	arg1	HSV-1					1374:1378	HSV-1	1374:1378	HSV-1	1374:1378	Furthermore, maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads was (31.25 μg ml-1 ) which exhibited promising antiviral activity against Herpes simplex virus-1 (HSV-1), and was significantly higher than chitosan/carrageenan without clove oil, where antiviral activity was 82.94% and 57.64%, respectively.
34743417	7	72	theme	Herpes	1350:1355	arg1	virus-1					1365:1371	Herpes simplex virus-1	1350:1371	Herpes simplex virus-1 (HSV-1)	1350:1379	Furthermore, maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads was (31.25 μg ml-1 ) which exhibited promising antiviral activity against Herpes simplex virus-1 (HSV-1), and was significantly higher than chitosan/carrageenan without clove oil, where antiviral activity was 82.94% and 57.64%, respectively.
34743417	2	73	theme	carrageenan	377:387	arg1	chitosan					364:371	chitosan	364:371	chitosan	364:371	A promising composite of chitosan and carrageenan with self-crosslinking has been prepared.
34743417	2	73	theme	carrageenan	377:387	arg1	composite					351:359	A promising composite	339:359	A promising composite of chitosan and carrageenan with self-crosslinking	339:410	A promising composite of chitosan and carrageenan with self-crosslinking has been prepared.
34743417	2	73	theme	carrageenan	377:387	arg1	carrageenan					377:387	carrageenan	377:387	carrageenan	377:387	A promising composite of chitosan and carrageenan with self-crosslinking has been prepared.
34743417	6	74	theme	chitosan/carrageenan	903:922	arg1	beads					934:938	chitosan/carrageenan@clove oil beads	903:938	chitosan/carrageenan@clove oil beads	903:938	Antibacterial results revealed that chitosan/carrageenan@clove oil beads have antimicrobial activity as well as chitosan/carrageenan without clove oil against Escherichia coli AATCC25922, Pseudomonas aeruginosa AATCC27853, Staphylococcus aureus ATCC25923, Bacillus subtilis AATCC6051, and Candida albicans ATCC90028.
34743417	6	75	theme	oil	930:932	arg1	beads					934:938	chitosan/carrageenan@clove oil beads	903:938	chitosan/carrageenan@clove oil beads	903:938	Antibacterial results revealed that chitosan/carrageenan@clove oil beads have antimicrobial activity as well as chitosan/carrageenan without clove oil against Escherichia coli AATCC25922, Pseudomonas aeruginosa AATCC27853, Staphylococcus aureus ATCC25923, Bacillus subtilis AATCC6051, and Candida albicans ATCC90028.
34743417	5	76	theme	antiviral	809:817	arg1	activity					819:826	antiviral activity	809:826	antiviral activity	809:826	Moreover, antimicrobial, antiviral activity, and molecular docking were evaluated.
34743417	3	77	theme	different	534:542	arg1	amounts					544:550	different amounts	534:550	different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared	534:663	METHODS AND RESULTS In this study, a rigorous approach for an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared.
34743417	3	77	theme	different	534:542	arg1	oil					561:563	clove oil	555:563	clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared	555:663	METHODS AND RESULTS In this study, a rigorous approach for an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared.
34743417	6	78	theme	antimicrobial	945:957	arg1	activity					959:966	antimicrobial activity	945:966	antimicrobial activity as well as chitosan/carrageenan	945:998	Antibacterial results revealed that chitosan/carrageenan@clove oil beads have antimicrobial activity as well as chitosan/carrageenan without clove oil against Escherichia coli AATCC25922, Pseudomonas aeruginosa AATCC27853, Staphylococcus aureus ATCC25923, Bacillus subtilis AATCC6051, and Candida albicans ATCC90028.
34743417	1	79	theme	distinctive	250:260	arg1	biocompatibility					307:322	biocompatibility	307:322	biocompatibility	307:322	BACKGROUND Biopolymers are promising candidates that can be fabricated into hydrophilic matrices and used for many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost.
34743417	1	79	theme	distinctive	250:260	arg1	non-toxic					281:289	non-toxic	281:289	non-toxic	281:289	BACKGROUND Biopolymers are promising candidates that can be fabricated into hydrophilic matrices and used for many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost.
34743417	1	79	theme	distinctive	250:260	arg1	biodegradable					292:304	biodegradable	292:304	biodegradable	292:304	BACKGROUND Biopolymers are promising candidates that can be fabricated into hydrophilic matrices and used for many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost.
34743417	1	79	theme	distinctive	250:260	arg1	properties					262:271	their distinctive properties	244:271	their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost	244:336	BACKGROUND Biopolymers are promising candidates that can be fabricated into hydrophilic matrices and used for many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost.
34743417	1	79	theme	distinctive	250:260	arg1	cost					333:336	low cost	329:336	low cost	329:336	BACKGROUND Biopolymers are promising candidates that can be fabricated into hydrophilic matrices and used for many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost.
34015714	3	0	with	spherical	629:637	arg1	structure					651:659	porous structure	644:659	porous structure	644:659	The SEM images revealed that the synthesized composite beads were spherical with porous structure.
34015714	5	1	theme	composite	765:773	arg1	beads					775:779	the CMC-C-Ni composite beads	752:779	the CMC-C-Ni composite beads	752:779	The physical characteristics of the CMC-C-Ni composite beads including wet density, porosity, and water content were respectively in the ranges of 1.23-1.63 g/cm3, 82.29-90.75%, and 52-76%.
34015714	5	2	theme	%	895:895	arg1	ranges					857:862	the ranges	853:862	the ranges of 1.23-1.63 g/cm3, 82.29-90.75%, and 52-76%	853:907	The physical characteristics of the CMC-C-Ni composite beads including wet density, porosity, and water content were respectively in the ranges of 1.23-1.63 g/cm3, 82.29-90.75%, and 52-76%.
34015714	5	3	theme	beads	775:779	arg1	characteristics					733:747	The physical characteristics	720:747	The physical characteristics of the CMC-C-Ni composite beads including wet density, porosity, and water content	720:830	The physical characteristics of the CMC-C-Ni composite beads including wet density, porosity, and water content were respectively in the ranges of 1.23-1.63 g/cm3, 82.29-90.75%, and 52-76%.
34015714	5	3	theme	beads	775:779	arg1	density					795:801	wet density	791:801	wet density	791:801	The physical characteristics of the CMC-C-Ni composite beads including wet density, porosity, and water content were respectively in the ranges of 1.23-1.63 g/cm3, 82.29-90.75%, and 52-76%.
34015714	5	3	theme	beads	775:779	arg1	content					824:830	water content	818:830	water content	818:830	The physical characteristics of the CMC-C-Ni composite beads including wet density, porosity, and water content were respectively in the ranges of 1.23-1.63 g/cm3, 82.29-90.75%, and 52-76%.
34015714	5	3	theme	beads	775:779	arg1	porosity					804:811	porosity	804:811	porosity	804:811	The physical characteristics of the CMC-C-Ni composite beads including wet density, porosity, and water content were respectively in the ranges of 1.23-1.63 g/cm3, 82.29-90.75%, and 52-76%.
34015714	1	4	theme	spherical	167:175	arg1	CMC-C-Ni					219:226	CMC-C-Ni	219:226	CMC-C-Ni	219:226	In the present work, spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads as novel adsorbent was synthesized to make a stable expanded bed adsorption (EBA) column for the treatment of the oily wastewater collected from the downstream of rapeseed industry.
34015714	1	4	theme	spherical	167:175	arg1	cellulose-cellulose-nickel					191:216	spherical carboxymethyl cellulose-cellulose-nickel	167:216	spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads as novel adsorbent	167:262	In the present work, spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads as novel adsorbent was synthesized to make a stable expanded bed adsorption (EBA) column for the treatment of the oily wastewater collected from the downstream of rapeseed industry.
34015714	0	5	theme	carboxymethyl	88:100	arg1	beads					139:143	carboxymethyl cellulose-cellulose-nickel composite beads	88:143	carboxymethyl cellulose-cellulose-nickel composite beads	88:143	Treatment of nano-oil polluted wastewater in an expanded bed adsorption column based on carboxymethyl cellulose-cellulose-nickel composite beads.
34015714	0	6	from	Treatment	0:8	arg1	column					72:77	an expanded bed adsorption column	45:77	an expanded bed adsorption column	45:77	Treatment of nano-oil polluted wastewater in an expanded bed adsorption column based on carboxymethyl cellulose-cellulose-nickel composite beads.
34015714	7	7	theme	settling	1078:1085	arg1	velocity					1087:1094	the terminal settling velocity	1065:1094	the terminal settling velocity (Ut)	1065:1099	The results showed that by increasing the fluid viscosity, the terminal settling velocity (Ut) was reduced.
34015714	7	7	theme	settling	1078:1085	arg1	Ut					1097:1098	Ut	1097:1098	Ut	1097:1098	The results showed that by increasing the fluid viscosity, the terminal settling velocity (Ut) was reduced.
34015714	6	8	theme	2-3	954:956	arg1	range					945:949	the range	941:949	the range of 2-3	941:956	The factor of bed expansion in the range of 2-3 was corresponded with Richardson-Zaki equation.
34015714	5	9	theme	physical	724:731	arg1	characteristics					733:747	The physical characteristics	720:747	The physical characteristics of the CMC-C-Ni composite beads including wet density, porosity, and water content	720:830	The physical characteristics of the CMC-C-Ni composite beads including wet density, porosity, and water content were respectively in the ranges of 1.23-1.63 g/cm3, 82.29-90.75%, and 52-76%.
34015714	5	9	theme	physical	724:731	arg1	density					795:801	wet density	791:801	wet density	791:801	The physical characteristics of the CMC-C-Ni composite beads including wet density, porosity, and water content were respectively in the ranges of 1.23-1.63 g/cm3, 82.29-90.75%, and 52-76%.
34015714	5	9	theme	physical	724:731	arg1	content					824:830	water content	818:830	water content	818:830	The physical characteristics of the CMC-C-Ni composite beads including wet density, porosity, and water content were respectively in the ranges of 1.23-1.63 g/cm3, 82.29-90.75%, and 52-76%.
34015714	5	9	theme	physical	724:731	arg1	porosity					804:811	porosity	804:811	porosity	804:811	The physical characteristics of the CMC-C-Ni composite beads including wet density, porosity, and water content were respectively in the ranges of 1.23-1.63 g/cm3, 82.29-90.75%, and 52-76%.
34015714	1	10	theme	oily	359:362	arg1	wastewater					364:373	the oily wastewater	355:373	the oily wastewater collected from the downstream of rapeseed industry	355:424	In the present work, spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads as novel adsorbent was synthesized to make a stable expanded bed adsorption (EBA) column for the treatment of the oily wastewater collected from the downstream of rapeseed industry.
34015714	1	11	theme	stable	290:295	arg1	column					327:332	a stable expanded bed adsorption (EBA) column	288:332	a stable expanded bed adsorption (EBA) column for the treatment of the oily wastewater collected from the downstream of rapeseed industry	288:424	In the present work, spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads as novel adsorbent was synthesized to make a stable expanded bed adsorption (EBA) column for the treatment of the oily wastewater collected from the downstream of rapeseed industry.
34015714	0	12	theme	composite	129:137	arg1	beads					139:143	carboxymethyl cellulose-cellulose-nickel composite beads	88:143	carboxymethyl cellulose-cellulose-nickel composite beads	88:143	Treatment of nano-oil polluted wastewater in an expanded bed adsorption column based on carboxymethyl cellulose-cellulose-nickel composite beads.
34015714	1	13	theme	wastewater	364:373	arg1	treatment					342:350	the treatment	338:350	the treatment of the oily wastewater collected from the downstream of rapeseed industry	338:424	In the present work, spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads as novel adsorbent was synthesized to make a stable expanded bed adsorption (EBA) column for the treatment of the oily wastewater collected from the downstream of rapeseed industry.
34015714	8	14	theme	utilized	1201:1208	arg1	index					1210:1214	commonly utilized index	1192:1214	commonly utilized index in the laminar flow regimes	1192:1242	The expansion index values were between 2.77 and 3.14 that were close to 4.8 (commonly utilized index in the laminar flow regimes).
34015714	1	15	theme	carboxymethyl	177:189	arg1	CMC-C-Ni					219:226	CMC-C-Ni	219:226	CMC-C-Ni	219:226	In the present work, spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads as novel adsorbent was synthesized to make a stable expanded bed adsorption (EBA) column for the treatment of the oily wastewater collected from the downstream of rapeseed industry.
34015714	1	15	theme	carboxymethyl	177:189	arg1	cellulose-cellulose-nickel					191:216	spherical carboxymethyl cellulose-cellulose-nickel	167:216	spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads as novel adsorbent	167:262	In the present work, spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads as novel adsorbent was synthesized to make a stable expanded bed adsorption (EBA) column for the treatment of the oily wastewater collected from the downstream of rapeseed industry.
34015714	0	16	theme	cellulose-cellulose-nickel	102:127	arg1	beads					139:143	carboxymethyl cellulose-cellulose-nickel composite beads	88:143	carboxymethyl cellulose-cellulose-nickel composite beads	88:143	Treatment of nano-oil polluted wastewater in an expanded bed adsorption column based on carboxymethyl cellulose-cellulose-nickel composite beads.
34015714	9	17	theme	composite	1255:1263	arg1	beads					1265:1269	CMC-C-Ni composite beads	1246:1269	CMC-C-Ni composite beads	1246:1269	CMC-C-Ni composite beads were tested when the velocity of fluid was ˂ 700 cm/h, and the Daxl was found to be ˂ 1 × 10-5 m2/s (steady state).
34015714	7	18	theme	terminal	1069:1076	arg1	velocity					1087:1094	the terminal settling velocity	1065:1094	the terminal settling velocity (Ut)	1065:1099	The results showed that by increasing the fluid viscosity, the terminal settling velocity (Ut) was reduced.
34015714	7	18	theme	terminal	1069:1076	arg1	Ut					1097:1098	Ut	1097:1098	Ut	1097:1098	The results showed that by increasing the fluid viscosity, the terminal settling velocity (Ut) was reduced.
34015714	1	19	theme	cellulose-cellulose-nickel	191:216	arg1	beads					239:243	spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads	167:243	spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads as novel adsorbent	167:262	In the present work, spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads as novel adsorbent was synthesized to make a stable expanded bed adsorption (EBA) column for the treatment of the oily wastewater collected from the downstream of rapeseed industry.
34015714	2	20	theme	electron	513:520	arg1	SEM					534:536	SEM	534:536	SEM	534:536	The morphology and structure of the CMC-C-Ni composite beads were studied by scanning electron microscopy (SEM) and optical microscope.
34015714	2	20	theme	electron	513:520	arg1	microscopy					522:531	scanning electron microscopy	504:531	scanning electron microscopy (SEM)	504:537	The morphology and structure of the CMC-C-Ni composite beads were studied by scanning electron microscopy (SEM) and optical microscope.
34015714	9	21	theme	CMC-C-Ni	1246:1253	arg1	beads					1265:1269	CMC-C-Ni composite beads	1246:1269	CMC-C-Ni composite beads	1246:1269	CMC-C-Ni composite beads were tested when the velocity of fluid was ˂ 700 cm/h, and the Daxl was found to be ˂ 1 × 10-5 m2/s (steady state).
34015714	9	22	theme	˂	1355:1355	arg1	Daxl					1334:1337	the Daxl	1330:1337	the Daxl	1330:1337	CMC-C-Ni composite beads were tested when the velocity of fluid was ˂ 700 cm/h, and the Daxl was found to be ˂ 1 × 10-5 m2/s (steady state).
34015714	9	22	theme	˂	1355:1355	arg1	m2/s					1366:1369	˂ 1 × 10-5 m2/s	1355:1369	˂ 1 × 10-5 m2/s (steady state)	1355:1384	CMC-C-Ni composite beads were tested when the velocity of fluid was ˂ 700 cm/h, and the Daxl was found to be ˂ 1 × 10-5 m2/s (steady state).
34015714	9	22	theme	˂	1355:1355	arg1	state					1379:1383	steady state	1372:1383	steady state	1372:1383	CMC-C-Ni composite beads were tested when the velocity of fluid was ˂ 700 cm/h, and the Daxl was found to be ˂ 1 × 10-5 m2/s (steady state).
34015714	1	23	theme	expanded	297:304	arg1	column					327:332	a stable expanded bed adsorption (EBA) column	288:332	a stable expanded bed adsorption (EBA) column for the treatment of the oily wastewater collected from the downstream of rapeseed industry	288:424	In the present work, spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads as novel adsorbent was synthesized to make a stable expanded bed adsorption (EBA) column for the treatment of the oily wastewater collected from the downstream of rapeseed industry.
34015714	4	24	theme	90-200 nm	709:717	arg1	range					700:704	the range	696:704	the range of 90-200 nm	696:717	The pore size of the beads was in the range of 90-200 nm.
34015714	5	25	theme	1.23-1.63 g/cm3	867:881	arg1	ranges					857:862	the ranges	853:862	the ranges of 1.23-1.63 g/cm3, 82.29-90.75%, and 52-76%	853:907	The physical characteristics of the CMC-C-Ni composite beads including wet density, porosity, and water content were respectively in the ranges of 1.23-1.63 g/cm3, 82.29-90.75%, and 52-76%.
34015714	0	26	theme	polluted	22:29	arg1	wastewater					31:40	nano-oil polluted wastewater	13:40	nano-oil polluted wastewater	13:40	Treatment of nano-oil polluted wastewater in an expanded bed adsorption column based on carboxymethyl cellulose-cellulose-nickel composite beads.
34015714	1	27	theme	bed	306:308	arg1	adsorption					310:319	bed adsorption	306:319	a stable expanded bed adsorption (EBA) column for the treatment of the oily wastewater collected from the downstream of rapeseed industry	288:424	In the present work, spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads as novel adsorbent was synthesized to make a stable expanded bed adsorption (EBA) column for the treatment of the oily wastewater collected from the downstream of rapeseed industry.
34015714	1	27	theme	bed	306:308	arg1	EBA					322:324	EBA	322:324	EBA	322:324	In the present work, spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads as novel adsorbent was synthesized to make a stable expanded bed adsorption (EBA) column for the treatment of the oily wastewater collected from the downstream of rapeseed industry.
34015714	8	28	dep	4.8	1187:1189	arg1	index					1210:1214	commonly utilized index	1192:1214	commonly utilized index in the laminar flow regimes	1192:1242	The expansion index values were between 2.77 and 3.14 that were close to 4.8 (commonly utilized index in the laminar flow regimes).
34015714	0	29	theme	nano-oil	13:20	arg1	wastewater					31:40	nano-oil polluted wastewater	13:40	nano-oil polluted wastewater	13:40	Treatment of nano-oil polluted wastewater in an expanded bed adsorption column based on carboxymethyl cellulose-cellulose-nickel composite beads.
34015714	1	30	theme	adsorption	310:319	arg1	column					327:332	a stable expanded bed adsorption (EBA) column	288:332	a stable expanded bed adsorption (EBA) column for the treatment of the oily wastewater collected from the downstream of rapeseed industry	288:424	In the present work, spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads as novel adsorbent was synthesized to make a stable expanded bed adsorption (EBA) column for the treatment of the oily wastewater collected from the downstream of rapeseed industry.
34015714	7	31	theme	fluid	1048:1052	arg1	viscosity					1054:1062	the fluid viscosity	1044:1062	the fluid viscosity	1044:1062	The results showed that by increasing the fluid viscosity, the terminal settling velocity (Ut) was reduced.
34015714	3	32	theme	SEM	567:569	arg1	images					571:576	The SEM images	563:576	The SEM images	563:576	The SEM images revealed that the synthesized composite beads were spherical with porous structure.
34015714	2	33	theme	composite	472:480	arg1	beads					482:486	the CMC-C-Ni composite beads	459:486	the CMC-C-Ni composite beads	459:486	The morphology and structure of the CMC-C-Ni composite beads were studied by scanning electron microscopy (SEM) and optical microscope.
34015714	1	34	theme	composite	229:237	arg1	beads					239:243	spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads	167:243	spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads as novel adsorbent	167:262	In the present work, spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads as novel adsorbent was synthesized to make a stable expanded bed adsorption (EBA) column for the treatment of the oily wastewater collected from the downstream of rapeseed industry.
34015714	6	35	theme	expansion	928:936	arg1	factor					914:919	The factor	910:919	The factor of bed expansion in the range of 2-3	910:956	The factor of bed expansion in the range of 2-3 was corresponded with Richardson-Zaki equation.
34015714	2	36	theme	optical	543:549	arg1	microscope					551:560	optical microscope	543:560	optical microscope	543:560	The morphology and structure of the CMC-C-Ni composite beads were studied by scanning electron microscopy (SEM) and optical microscope.
34015714	3	37	theme	synthesized	596:606	arg1	spherical					629:637	spherical	629:637	spherical	629:637	The SEM images revealed that the synthesized composite beads were spherical with porous structure.
34015714	3	37	theme	synthesized	596:606	arg1	beads					618:622	the synthesized composite beads	592:622	the synthesized composite beads	592:622	The SEM images revealed that the synthesized composite beads were spherical with porous structure.
34015714	9	38	theme	1 × 10-5	1357:1364	arg1	Daxl					1334:1337	the Daxl	1330:1337	the Daxl	1330:1337	CMC-C-Ni composite beads were tested when the velocity of fluid was ˂ 700 cm/h, and the Daxl was found to be ˂ 1 × 10-5 m2/s (steady state).
34015714	9	38	theme	1 × 10-5	1357:1364	arg1	m2/s					1366:1369	˂ 1 × 10-5 m2/s	1355:1369	˂ 1 × 10-5 m2/s (steady state)	1355:1384	CMC-C-Ni composite beads were tested when the velocity of fluid was ˂ 700 cm/h, and the Daxl was found to be ˂ 1 × 10-5 m2/s (steady state).
34015714	9	38	theme	1 × 10-5	1357:1364	arg1	state					1379:1383	steady state	1372:1383	steady state	1372:1383	CMC-C-Ni composite beads were tested when the velocity of fluid was ˂ 700 cm/h, and the Daxl was found to be ˂ 1 × 10-5 m2/s (steady state).
34015714	9	39	theme	steady	1372:1377	arg1	m2/s					1366:1369	˂ 1 × 10-5 m2/s	1355:1369	˂ 1 × 10-5 m2/s (steady state)	1355:1384	CMC-C-Ni composite beads were tested when the velocity of fluid was ˂ 700 cm/h, and the Daxl was found to be ˂ 1 × 10-5 m2/s (steady state).
34015714	9	39	theme	steady	1372:1377	arg1	state					1379:1383	steady state	1372:1383	steady state	1372:1383	CMC-C-Ni composite beads were tested when the velocity of fluid was ˂ 700 cm/h, and the Daxl was found to be ˂ 1 × 10-5 m2/s (steady state).
34015714	0	40	theme	wastewater	31:40	arg1	Treatment					0:8	Treatment	0:8	Treatment of nano-oil polluted wastewater in an expanded bed adsorption column	0:77	Treatment of nano-oil polluted wastewater in an expanded bed adsorption column based on carboxymethyl cellulose-cellulose-nickel composite beads.
34015714	6	41	theme	bed	924:926	arg1	expansion					928:936	bed expansion	924:936	bed expansion	924:936	The factor of bed expansion in the range of 2-3 was corresponded with Richardson-Zaki equation.
34015714	2	42	theme	beads	482:486	arg1	structure					446:454	structure	446:454	structure	446:454	The morphology and structure of the CMC-C-Ni composite beads were studied by scanning electron microscopy (SEM) and optical microscope.
34015714	2	42	theme	beads	482:486	arg1	morphology					431:440	morphology	431:440	morphology	431:440	The morphology and structure of the CMC-C-Ni composite beads were studied by scanning electron microscopy (SEM) and optical microscope.
34015714	3	43	theme	porous	644:649	arg1	structure					651:659	porous structure	644:659	porous structure	644:659	The SEM images revealed that the synthesized composite beads were spherical with porous structure.
34015714	3	44	theme	composite	608:616	arg1	spherical					629:637	spherical	629:637	spherical	629:637	The SEM images revealed that the synthesized composite beads were spherical with porous structure.
34015714	3	44	theme	composite	608:616	arg1	beads					618:622	the synthesized composite beads	592:622	the synthesized composite beads	592:622	The SEM images revealed that the synthesized composite beads were spherical with porous structure.
34015714	8	45	theme	index	1128:1132	arg1	values					1134:1139	The expansion index values	1114:1139	The expansion index values	1114:1139	The expansion index values were between 2.77 and 3.14 that were close to 4.8 (commonly utilized index in the laminar flow regimes).
34015714	0	46	theme	expanded	48:55	arg1	column					72:77	an expanded bed adsorption column	45:77	an expanded bed adsorption column	45:77	Treatment of nano-oil polluted wastewater in an expanded bed adsorption column based on carboxymethyl cellulose-cellulose-nickel composite beads.
34015714	8	47	theme	expansion	1118:1126	arg1	values					1134:1139	The expansion index values	1114:1139	The expansion index values	1114:1139	The expansion index values were between 2.77 and 3.14 that were close to 4.8 (commonly utilized index in the laminar flow regimes).
34015714	6	48	from	factor	914:919	arg1	range					945:949	the range	941:949	the range of 2-3	941:956	The factor of bed expansion in the range of 2-3 was corresponded with Richardson-Zaki equation.
34015714	1	49	theme	rapeseed	408:415	arg1	industry					417:424	rapeseed industry	408:424	rapeseed industry	408:424	In the present work, spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads as novel adsorbent was synthesized to make a stable expanded bed adsorption (EBA) column for the treatment of the oily wastewater collected from the downstream of rapeseed industry.
34015714	2	50	dep	morphology	431:440	arg1	The					427:429	The	427:429	The	427:429	The morphology and structure of the CMC-C-Ni composite beads were studied by scanning electron microscopy (SEM) and optical microscope.
34015714	8	51	theme	flow	1231:1234	arg1	regimes					1236:1242	the laminar flow regimes	1219:1242	the laminar flow regimes	1219:1242	The expansion index values were between 2.77 and 3.14 that were close to 4.8 (commonly utilized index in the laminar flow regimes).
34015714	2	52	theme	CMC-C-Ni	463:470	arg1	beads					482:486	the CMC-C-Ni composite beads	459:486	the CMC-C-Ni composite beads	459:486	The morphology and structure of the CMC-C-Ni composite beads were studied by scanning electron microscopy (SEM) and optical microscope.
34015714	9	53	theme	fluid	1304:1308	arg1	velocity					1292:1299	the velocity	1288:1299	the velocity of fluid	1288:1308	CMC-C-Ni composite beads were tested when the velocity of fluid was ˂ 700 cm/h, and the Daxl was found to be ˂ 1 × 10-5 m2/s (steady state).
34015714	1	54	theme	industry	417:424	arg1	downstream					394:403	downstream	394:403	downstream	394:403	In the present work, spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads as novel adsorbent was synthesized to make a stable expanded bed adsorption (EBA) column for the treatment of the oily wastewater collected from the downstream of rapeseed industry.
34015714	8	55	theme	laminar	1223:1229	arg1	regimes					1236:1242	the laminar flow regimes	1219:1242	the laminar flow regimes	1219:1242	The expansion index values were between 2.77 and 3.14 that were close to 4.8 (commonly utilized index in the laminar flow regimes).
34015714	5	56	theme	CMC-C-Ni	756:763	arg1	beads					775:779	the CMC-C-Ni composite beads	752:779	the CMC-C-Ni composite beads	752:779	The physical characteristics of the CMC-C-Ni composite beads including wet density, porosity, and water content were respectively in the ranges of 1.23-1.63 g/cm3, 82.29-90.75%, and 52-76%.
34015714	5	57	theme	%	907:907	arg1	ranges					857:862	the ranges	853:862	the ranges of 1.23-1.63 g/cm3, 82.29-90.75%, and 52-76%	853:907	The physical characteristics of the CMC-C-Ni composite beads including wet density, porosity, and water content were respectively in the ranges of 1.23-1.63 g/cm3, 82.29-90.75%, and 52-76%.
34015714	5	58	theme	water	818:822	arg1	content					824:830	water content	818:830	water content	818:830	The physical characteristics of the CMC-C-Ni composite beads including wet density, porosity, and water content were respectively in the ranges of 1.23-1.63 g/cm3, 82.29-90.75%, and 52-76%.
34015714	2	59	theme	scanning	504:511	arg1	SEM					534:536	SEM	534:536	SEM	534:536	The morphology and structure of the CMC-C-Ni composite beads were studied by scanning electron microscopy (SEM) and optical microscope.
34015714	2	59	theme	scanning	504:511	arg1	microscopy					522:531	scanning electron microscopy	504:531	scanning electron microscopy (SEM)	504:537	The morphology and structure of the CMC-C-Ni composite beads were studied by scanning electron microscopy (SEM) and optical microscope.
34015714	0	60	theme	adsorption	61:70	arg1	column					72:77	an expanded bed adsorption column	45:77	an expanded bed adsorption column	45:77	Treatment of nano-oil polluted wastewater in an expanded bed adsorption column based on carboxymethyl cellulose-cellulose-nickel composite beads.
34015714	1	61	theme	present	153:159	arg1	work					161:164	the present work	149:164	the present work	149:164	In the present work, spherical carboxymethyl cellulose-cellulose-nickel (CMC-C-Ni) composite beads as novel adsorbent was synthesized to make a stable expanded bed adsorption (EBA) column for the treatment of the oily wastewater collected from the downstream of rapeseed industry.
34015714	4	62	theme	pore	666:669	arg1	size					671:674	The pore size	662:674	The pore size of the beads	662:687	The pore size of the beads was in the range of 90-200 nm.
34015714	6	63	theme	Richardson-Zaki	980:994	arg1	equation					996:1003	Richardson-Zaki equation	980:1003	Richardson-Zaki equation	980:1003	The factor of bed expansion in the range of 2-3 was corresponded with Richardson-Zaki equation.
34015714	0	64	theme	bed	57:59	arg1	column					72:77	an expanded bed adsorption column	45:77	an expanded bed adsorption column	45:77	Treatment of nano-oil polluted wastewater in an expanded bed adsorption column based on carboxymethyl cellulose-cellulose-nickel composite beads.
34015714	5	65	theme	wet	791:793	arg1	density					795:801	wet density	791:801	wet density	791:801	The physical characteristics of the CMC-C-Ni composite beads including wet density, porosity, and water content were respectively in the ranges of 1.23-1.63 g/cm3, 82.29-90.75%, and 52-76%.
34015714	8	66	from	index	1210:1214	arg1	regimes					1236:1242	the laminar flow regimes	1219:1242	the laminar flow regimes	1219:1242	The expansion index values were between 2.77 and 3.14 that were close to 4.8 (commonly utilized index in the laminar flow regimes).
34015714	4	67	theme	beads	683:687	arg1	size					671:674	The pore size	662:674	The pore size of the beads	662:687	The pore size of the beads was in the range of 90-200 nm.
34508964	7	0	theme	quadratic	1162:1170	arg1	model					1172:1176	quadratic model	1162:1176	quadratic model	1162:1176	The results revealed that quadratic model was proper for the prediction removal of malathion.
34508964	4	1	theme	response	851:858	arg1	RSM					881:883	RSM	881:883	RSM	881:883	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	1	theme	response	851:858	arg1	methodology					868:878	the response surface methodology	847:878	the response surface methodology (RSM)	847:884	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	2	2	theme	malathion	373:381	arg1	removal					362:368	the removal	358:368	the removal of malathion from agricultural effluents using microemulsion method	358:436	In this study, the low-cost easily accessible chitosan-alginate adsorbent was prepared for the removal of malathion from agricultural effluents using microemulsion method.
34508964	9	3	theme	 = 0.99	1361:1367	arg1	coefficient					1328:1338	a coefficient	1326:1338	a coefficient of determination (R2) = 0.99 and pseudo-second-order	1326:1391	The Langmuir with a coefficient of determination (R2) = 0.99 and pseudo-second-order with R2 = 0.99 were achieved as the best isotherm and kinetic models, respectively.
34508964	4	4	from	levels	777:782	arg1	0.05-0.25 g					685:695	0.05-0.25 g	685:695	0.05-0.25 g	685:695	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	4	from	levels	777:782	arg1	dosage					677:682	adsorbent dosage	667:682	adsorbent dosage (0.05-0.25 g)	667:696	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	4	from	levels	777:782	arg1	5-25 min					713:720	5-25 min	713:720	5-25 min	713:720	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	4	from	levels	777:782	arg1	time					707:710	contact time	699:710	contact time (5-25 min)	699:721	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	4	from	levels	777:782	arg1	concentration					728:740	concentration	728:740	concentration of malathion (5-25 mg L-1) at five levels	728:782	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	6	5	theme	variance	1053:1060	arg1	analysis					1041:1048	The analysis	1037:1048	The analysis of variance (ANOVA)	1037:1068	The analysis of variance (ANOVA) was applied to assess the significance and adequacy of the model.
34508964	9	6	with	Langmuir	1312:1319	arg1	R2 = 0.99					1398:1406	R2 = 0.99	1398:1406	R2 = 0.99	1398:1406	The Langmuir with a coefficient of determination (R2) = 0.99 and pseudo-second-order with R2 = 0.99 were achieved as the best isotherm and kinetic models, respectively.
34508964	9	6	with	Langmuir	1312:1319	arg1	coefficient					1328:1338	a coefficient	1326:1338	a coefficient of determination (R2) = 0.99 and pseudo-second-order	1326:1391	The Langmuir with a coefficient of determination (R2) = 0.99 and pseudo-second-order with R2 = 0.99 were achieved as the best isotherm and kinetic models, respectively.
34508964	3	7	theme	Fourier-transform	572:588	arg1	FTIR					613:616	FTIR	613:616	FTIR	613:616	The adsorbent was characterized using scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR).
34508964	3	7	theme	Fourier-transform	572:588	arg1	spectroscopy					599:610	Fourier-transform infrared spectroscopy	572:610	Fourier-transform infrared spectroscopy (FTIR)	572:617	The adsorbent was characterized using scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR).
34508964	9	8	theme	pseudo-second-order	1373:1391	arg1	coefficient					1328:1338	a coefficient	1326:1338	a coefficient of determination (R2) = 0.99 and pseudo-second-order	1326:1391	The Langmuir with a coefficient of determination (R2) = 0.99 and pseudo-second-order with R2 = 0.99 were achieved as the best isotherm and kinetic models, respectively.
34508964	4	9	theme	malathion	745:753	arg1	0.05-0.25 g					685:695	0.05-0.25 g	685:695	0.05-0.25 g	685:695	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	9	theme	malathion	745:753	arg1	dosage					677:682	adsorbent dosage	667:682	adsorbent dosage (0.05-0.25 g)	667:696	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	9	theme	malathion	745:753	arg1	5-25 min					713:720	5-25 min	713:720	5-25 min	713:720	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	9	theme	malathion	745:753	arg1	time					707:710	contact time	699:710	contact time (5-25 min)	699:721	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	9	theme	malathion	745:753	arg1	concentration					728:740	concentration	728:740	concentration of malathion (5-25 mg L-1) at five levels	728:782	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	10	theme	surface	860:866	arg1	RSM					881:883	RSM	881:883	RSM	881:883	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	10	theme	surface	860:866	arg1	methodology					868:878	the response surface methodology	847:878	the response surface methodology (RSM)	847:884	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	2	11	theme	low-cost	286:293	arg1	chitosan-alginate					313:329	the low-cost easily accessible chitosan-alginate adsorbent	282:339	the low-cost easily accessible chitosan-alginate adsorbent	282:339	In this study, the low-cost easily accessible chitosan-alginate adsorbent was prepared for the removal of malathion from agricultural effluents using microemulsion method.
34508964	7	12	theme	malathion	1219:1227	arg1	removal					1208:1214	the prediction removal	1193:1214	the prediction removal of malathion	1193:1227	The results revealed that quadratic model was proper for the prediction removal of malathion.
34508964	0	13	theme	surface	122:128	arg1	methodology					130:140	response surface methodology	113:140	response surface methodology	113:140	Application of chitosan-alginate bio composite for adsorption of malathion from wastewater: Characterization and response surface methodology.
34508964	7	14	theme	prediction	1197:1206	arg1	removal					1208:1214	the prediction removal	1193:1214	the prediction removal of malathion	1193:1227	The results revealed that quadratic model was proper for the prediction removal of malathion.
34508964	8	15	theme	adsorption	1234:1243	arg1	kinetics					1245:1252	The adsorption kinetics	1230:1252	The adsorption kinetics	1230:1252	The adsorption kinetics and isotherms were examined under optimal conditions.
34508964	5	16	theme	removal	899:905	arg1	percentage					907:916	The highest removal percentage	887:916	The highest removal percentage	887:916	The highest removal percentage was obtained 82.35 with an adsorbent dosage of 0.18 g, contact time of 20 min, and initial concentration of 10 mg L-1.
34508964	3	17	theme	scanning	477:484	arg1	SEM					507:509	SEM	507:509	SEM	507:509	The adsorbent was characterized using scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR).
34508964	3	17	theme	scanning	477:484	arg1	microscopy					495:504	scanning electron microscopy	477:504	scanning electron microscopy (SEM)	477:510	The adsorbent was characterized using scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR).
34508964	0	18	theme	response	113:120	arg1	methodology					130:140	response surface methodology	113:140	response surface methodology	113:140	Application of chitosan-alginate bio composite for adsorption of malathion from wastewater: Characterization and response surface methodology.
34508964	2	19	theme	microemulsion	417:429	arg1	method					431:436	microemulsion method	417:436	microemulsion method	417:436	In this study, the low-cost easily accessible chitosan-alginate adsorbent was prepared for the removal of malathion from agricultural effluents using microemulsion method.
34508964	1	20	from	effluents	156:164	arg1	media					177:181	aqueous media	169:181	aqueous media	169:181	Agricultural effluents in aqueous media have caused serious threats due to adversely affect human health and the ecosystem.
34508964	3	21	theme	infrared	590:597	arg1	FTIR					613:616	FTIR	613:616	FTIR	613:616	The adsorbent was characterized using scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR).
34508964	3	21	theme	infrared	590:597	arg1	spectroscopy					599:610	Fourier-transform infrared spectroscopy	572:610	Fourier-transform infrared spectroscopy (FTIR)	572:617	The adsorbent was characterized using scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR).
34508964	5	22	theme	10 mg L-1	1026:1034	arg1	0.18 g					965:970	0.18 g	965:970	0.18 g	965:970	The highest removal percentage was obtained 82.35 with an adsorbent dosage of 0.18 g, contact time of 20 min, and initial concentration of 10 mg L-1.
34508964	5	22	theme	10 mg L-1	1026:1034	arg1	time					981:984	contact time	973:984	contact time of 20 min	973:994	The highest removal percentage was obtained 82.35 with an adsorbent dosage of 0.18 g, contact time of 20 min, and initial concentration of 10 mg L-1.
34508964	5	22	theme	10 mg L-1	1026:1034	arg1	concentration					1009:1021	initial concentration	1001:1021	initial concentration of 10 mg L-1	1001:1034	The highest removal percentage was obtained 82.35 with an adsorbent dosage of 0.18 g, contact time of 20 min, and initial concentration of 10 mg L-1.
34508964	2	23	theme	adsorbent	331:339	arg1	chitosan-alginate					313:329	the low-cost easily accessible chitosan-alginate adsorbent	282:339	the low-cost easily accessible chitosan-alginate adsorbent	282:339	In this study, the low-cost easily accessible chitosan-alginate adsorbent was prepared for the removal of malathion from agricultural effluents using microemulsion method.
34508964	1	24	theme	due	211:213	arg1	threats					203:209	serious threats	195:209	serious threats due to adversely affect human health and the ecosystem	195:264	Agricultural effluents in aqueous media have caused serious threats due to adversely affect human health and the ecosystem.
34508964	0	25	theme	bio	33:35	arg1	composite					37:45	chitosan-alginate bio composite	15:45	chitosan-alginate bio composite	15:45	Application of chitosan-alginate bio composite for adsorption of malathion from wastewater: Characterization and response surface methodology.
34508964	9	26	theme	best	1429:1432	arg1	isotherm					1434:1441	the best isotherm	1425:1441	the best isotherm	1425:1441	The Langmuir with a coefficient of determination (R2) = 0.99 and pseudo-second-order with R2 = 0.99 were achieved as the best isotherm and kinetic models, respectively.
34508964	0	27	theme	chitosan-alginate	15:31	arg1	composite					37:45	chitosan-alginate bio composite	15:45	chitosan-alginate bio composite	15:45	Application of chitosan-alginate bio composite for adsorption of malathion from wastewater: Characterization and response surface methodology.
34508964	2	28	theme	accessible	302:311	arg1	chitosan-alginate					313:329	the low-cost easily accessible chitosan-alginate adsorbent	282:339	the low-cost easily accessible chitosan-alginate adsorbent	282:339	In this study, the low-cost easily accessible chitosan-alginate adsorbent was prepared for the removal of malathion from agricultural effluents using microemulsion method.
34508964	3	29	theme	X-ray	543:547	arg1	XRD					562:564	XRD	562:564	XRD	562:564	The adsorbent was characterized using scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR).
34508964	3	29	theme	X-ray	543:547	arg1	diffraction					549:559	X-ray diffraction	543:559	X-ray diffraction (XRD)	543:565	The adsorbent was characterized using scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR).
34508964	5	30	theme	time	981:984	arg1	dosage					955:960	an adsorbent dosage	942:960	an adsorbent dosage of 0.18 g, contact time of 20 min, and initial concentration of 10 mg L-1	942:1034	The highest removal percentage was obtained 82.35 with an adsorbent dosage of 0.18 g, contact time of 20 min, and initial concentration of 10 mg L-1.
34508964	0	31	from	wastewater	80:89	arg1	adsorption					51:60	adsorption	51:60	adsorption of malathion from wastewater	51:89	Application of chitosan-alginate bio composite for adsorption of malathion from wastewater: Characterization and response surface methodology.
34508964	4	32	theme	central	817:823	arg1	CCD					833:835	CCD	833:835	CCD	833:835	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	32	theme	central	817:823	arg1	design					825:830	the composite central design	803:830	the composite central design (CCD) based on the response surface methodology (RSM)	803:884	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	10	33	theme	aqueous	1610:1616	arg1	solution					1618:1625	aqueous solution	1610:1625	aqueous solution	1610:1625	The results showed that the chitosan-alginate biopolymer can be effective and affordable adsorbent for the removal of malathion from aqueous solution.
34508964	9	34	theme	isotherm	1434:1441	arg1	Langmuir					1312:1319	The Langmuir	1308:1319	The Langmuir with a coefficient of determination (R2) = 0.99 and pseudo-second-order with R2 = 0.99	1308:1406	The Langmuir with a coefficient of determination (R2) = 0.99 and pseudo-second-order with R2 = 0.99 were achieved as the best isotherm and kinetic models, respectively.
34508964	9	34	theme	isotherm	1434:1441	arg1	models					1455:1460	the best isotherm and kinetic models	1425:1460	the best isotherm and kinetic models	1425:1460	The Langmuir with a coefficient of determination (R2) = 0.99 and pseudo-second-order with R2 = 0.99 were achieved as the best isotherm and kinetic models, respectively.
34508964	0	35	theme	composite	37:45	arg1	Application					0:10	Application	0:10	Application of chitosan-alginate bio composite for adsorption of malathion from wastewater: Characterization and response surface methodology.	0:141	Application of chitosan-alginate bio composite for adsorption of malathion from wastewater: Characterization and response surface methodology.
34508964	10	36	theme	malathion	1595:1603	arg1	removal					1584:1590	the removal	1580:1590	the removal of malathion from aqueous solution	1580:1625	The results showed that the chitosan-alginate biopolymer can be effective and affordable adsorbent for the removal of malathion from aqueous solution.
34508964	4	37	theme	composite	807:815	arg1	CCD					833:835	CCD	833:835	CCD	833:835	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	37	theme	composite	807:815	arg1	design					825:830	the composite central design	803:830	the composite central design (CCD) based on the response surface methodology (RSM)	803:884	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	10	38	from	solution	1618:1625	arg1	removal					1584:1590	the removal	1580:1590	the removal of malathion from aqueous solution	1580:1625	The results showed that the chitosan-alginate biopolymer can be effective and affordable adsorbent for the removal of malathion from aqueous solution.
34508964	1	39	theme	human	235:239	arg1	health					241:246	human health	235:246	human health	235:246	Agricultural effluents in aqueous media have caused serious threats due to adversely affect human health and the ecosystem.
34508964	10	40	theme	chitosan-alginate	1505:1521	arg1	biopolymer					1523:1532	the chitosan-alginate biopolymer	1501:1532	the chitosan-alginate biopolymer	1501:1532	The results showed that the chitosan-alginate biopolymer can be effective and affordable adsorbent for the removal of malathion from aqueous solution.
34508964	10	40	theme	chitosan-alginate	1505:1521	arg1	effective					1541:1549	effective	1541:1549	effective	1541:1549	The results showed that the chitosan-alginate biopolymer can be effective and affordable adsorbent for the removal of malathion from aqueous solution.
34508964	10	40	theme	chitosan-alginate	1505:1521	arg1	adsorbent					1566:1574	adsorbent	1566:1574	adsorbent	1566:1574	The results showed that the chitosan-alginate biopolymer can be effective and affordable adsorbent for the removal of malathion from aqueous solution.
34508964	5	41	theme	adsorbent	945:953	arg1	dosage					955:960	an adsorbent dosage	942:960	an adsorbent dosage of 0.18 g, contact time of 20 min, and initial concentration of 10 mg L-1	942:1034	The highest removal percentage was obtained 82.35 with an adsorbent dosage of 0.18 g, contact time of 20 min, and initial concentration of 10 mg L-1.
34508964	2	42	from	effluents	401:409	arg1	removal					362:368	the removal	358:368	the removal of malathion from agricultural effluents using microemulsion method	358:436	In this study, the low-cost easily accessible chitosan-alginate adsorbent was prepared for the removal of malathion from agricultural effluents using microemulsion method.
34508964	9	43	theme	kinetic	1447:1453	arg1	Langmuir					1312:1319	The Langmuir	1308:1319	The Langmuir with a coefficient of determination (R2) = 0.99 and pseudo-second-order with R2 = 0.99	1308:1406	The Langmuir with a coefficient of determination (R2) = 0.99 and pseudo-second-order with R2 = 0.99 were achieved as the best isotherm and kinetic models, respectively.
34508964	9	43	theme	kinetic	1447:1453	arg1	models					1455:1460	the best isotherm and kinetic models	1425:1460	the best isotherm and kinetic models	1425:1460	The Langmuir with a coefficient of determination (R2) = 0.99 and pseudo-second-order with R2 = 0.99 were achieved as the best isotherm and kinetic models, respectively.
34508964	10	44	dep	effective	1541:1549	arg1	biopolymer					1523:1532	the chitosan-alginate biopolymer	1501:1532	the chitosan-alginate biopolymer	1501:1532	The results showed that the chitosan-alginate biopolymer can be effective and affordable adsorbent for the removal of malathion from aqueous solution.
34508964	10	44	dep	effective	1541:1549	arg1	effective					1541:1549	effective	1541:1549	effective	1541:1549	The results showed that the chitosan-alginate biopolymer can be effective and affordable adsorbent for the removal of malathion from aqueous solution.
34508964	10	44	dep	effective	1541:1549	arg1	adsorbent					1566:1574	adsorbent	1566:1574	adsorbent	1566:1574	The results showed that the chitosan-alginate biopolymer can be effective and affordable adsorbent for the removal of malathion from aqueous solution.
34508964	6	45	theme	model	1129:1133	arg1	adequacy					1113:1120	adequacy	1113:1120	adequacy	1113:1120	The analysis of variance (ANOVA) was applied to assess the significance and adequacy of the model.
34508964	6	45	theme	model	1129:1133	arg1	significance					1096:1107	significance	1096:1107	significance	1096:1107	The analysis of variance (ANOVA) was applied to assess the significance and adequacy of the model.
34508964	5	46	theme	initial	1001:1007	arg1	concentration					1009:1021	initial concentration	1001:1021	initial concentration of 10 mg L-1	1001:1034	The highest removal percentage was obtained 82.35 with an adsorbent dosage of 0.18 g, contact time of 20 min, and initial concentration of 10 mg L-1.
34508964	5	47	theme	highest	891:897	arg1	percentage					907:916	The highest removal percentage	887:916	The highest removal percentage	887:916	The highest removal percentage was obtained 82.35 with an adsorbent dosage of 0.18 g, contact time of 20 min, and initial concentration of 10 mg L-1.
34508964	0	48	dep	Application	0:10	arg1	methodology					130:140	response surface methodology	113:140	response surface methodology	113:140	Application of chitosan-alginate bio composite for adsorption of malathion from wastewater: Characterization and response surface methodology.
34508964	0	48	dep	Application	0:10	arg1	Characterization					92:107	Characterization	92:107	Characterization	92:107	Application of chitosan-alginate bio composite for adsorption of malathion from wastewater: Characterization and response surface methodology.
34508964	5	49	theme	concentration	1009:1021	arg1	dosage					955:960	an adsorbent dosage	942:960	an adsorbent dosage of 0.18 g, contact time of 20 min, and initial concentration of 10 mg L-1	942:1034	The highest removal percentage was obtained 82.35 with an adsorbent dosage of 0.18 g, contact time of 20 min, and initial concentration of 10 mg L-1.
34508964	5	50	theme	0.18 g	965:970	arg1	dosage					955:960	an adsorbent dosage	942:960	an adsorbent dosage of 0.18 g, contact time of 20 min, and initial concentration of 10 mg L-1	942:1034	The highest removal percentage was obtained 82.35 with an adsorbent dosage of 0.18 g, contact time of 20 min, and initial concentration of 10 mg L-1.
34508964	5	51	theme	20 min	989:994	arg1	0.18 g					965:970	0.18 g	965:970	0.18 g	965:970	The highest removal percentage was obtained 82.35 with an adsorbent dosage of 0.18 g, contact time of 20 min, and initial concentration of 10 mg L-1.
34508964	5	51	theme	20 min	989:994	arg1	time					981:984	contact time	973:984	contact time of 20 min	973:994	The highest removal percentage was obtained 82.35 with an adsorbent dosage of 0.18 g, contact time of 20 min, and initial concentration of 10 mg L-1.
34508964	5	51	theme	20 min	989:994	arg1	concentration					1009:1021	initial concentration	1001:1021	initial concentration of 10 mg L-1	1001:1034	The highest removal percentage was obtained 82.35 with an adsorbent dosage of 0.18 g, contact time of 20 min, and initial concentration of 10 mg L-1.
34508964	1	52	theme	Agricultural	143:154	arg1	effluents					156:164	Agricultural effluents	143:164	Agricultural effluents in aqueous media	143:181	Agricultural effluents in aqueous media have caused serious threats due to adversely affect human health and the ecosystem.
34508964	9	53	theme	determination	1343:1355	arg1	 = 0.99					1361:1367	determination (R2) = 0.99	1343:1367	determination (R2) = 0.99	1343:1367	The Langmuir with a coefficient of determination (R2) = 0.99 and pseudo-second-order with R2 = 0.99 were achieved as the best isotherm and kinetic models, respectively.
34508964	4	54	theme	optimum	624:630	arg1	time					707:710	contact time	699:710	contact time (5-25 min)	699:721	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	54	theme	optimum	624:630	arg1	concentration					728:740	concentration	728:740	concentration of malathion (5-25 mg L-1) at five levels	728:782	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	54	theme	optimum	624:630	arg1	dosage					677:682	adsorbent dosage	667:682	adsorbent dosage (0.05-0.25 g)	667:696	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	54	theme	optimum	624:630	arg1	conditions					645:654	The optimum experimental conditions	620:654	The optimum experimental conditions	620:654	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	55	theme	contact	699:705	arg1	5-25 min					713:720	5-25 min	713:720	5-25 min	713:720	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	55	theme	contact	699:705	arg1	time					707:710	contact time	699:710	contact time (5-25 min)	699:721	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	8	56	theme	optimal	1288:1294	arg1	conditions					1296:1305	optimal conditions	1288:1305	optimal conditions	1288:1305	The adsorption kinetics and isotherms were examined under optimal conditions.
34508964	3	57	theme	electron	486:493	arg1	SEM					507:509	SEM	507:509	SEM	507:509	The adsorbent was characterized using scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR).
34508964	3	57	theme	electron	486:493	arg1	microscopy					495:504	scanning electron microscopy	477:504	scanning electron microscopy (SEM)	477:510	The adsorbent was characterized using scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR).
34508964	4	58	theme	adsorbent	667:675	arg1	0.05-0.25 g					685:695	0.05-0.25 g	685:695	0.05-0.25 g	685:695	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	58	theme	adsorbent	667:675	arg1	dosage					677:682	adsorbent dosage	667:682	adsorbent dosage (0.05-0.25 g)	667:696	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	5	59	theme	contact	973:979	arg1	time					981:984	contact time	973:984	contact time of 20 min	973:994	The highest removal percentage was obtained 82.35 with an adsorbent dosage of 0.18 g, contact time of 20 min, and initial concentration of 10 mg L-1.
34508964	2	60	theme	agricultural	388:399	arg1	effluents					401:409	agricultural effluents	388:409	agricultural effluents using microemulsion method	388:436	In this study, the low-cost easily accessible chitosan-alginate adsorbent was prepared for the removal of malathion from agricultural effluents using microemulsion method.
34508964	1	61	theme	serious	195:201	arg1	threats					203:209	serious threats	195:209	serious threats due to adversely affect human health and the ecosystem	195:264	Agricultural effluents in aqueous media have caused serious threats due to adversely affect human health and the ecosystem.
34508964	9	62	theme	R2	1358:1359	arg1	 = 0.99					1361:1367	determination (R2) = 0.99	1343:1367	determination (R2) = 0.99	1343:1367	The Langmuir with a coefficient of determination (R2) = 0.99 and pseudo-second-order with R2 = 0.99 were achieved as the best isotherm and kinetic models, respectively.
34508964	0	63	theme	malathion	65:73	arg1	adsorption					51:60	adsorption	51:60	adsorption of malathion from wastewater	51:89	Application of chitosan-alginate bio composite for adsorption of malathion from wastewater: Characterization and response surface methodology.
34508964	1	64	dep	adversely	218:226	arg1	affect					228:233	affect	228:233	affect human health and the ecosystem	228:264	Agricultural effluents in aqueous media have caused serious threats due to adversely affect human health and the ecosystem.
34508964	1	65	theme	aqueous	169:175	arg1	media					177:181	aqueous media	169:181	aqueous media	169:181	Agricultural effluents in aqueous media have caused serious threats due to adversely affect human health and the ecosystem.
34508964	4	66	theme	experimental	632:643	arg1	time					707:710	contact time	699:710	contact time (5-25 min)	699:721	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	66	theme	experimental	632:643	arg1	concentration					728:740	concentration	728:740	concentration of malathion (5-25 mg L-1) at five levels	728:782	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	66	theme	experimental	632:643	arg1	dosage					677:682	adsorbent dosage	667:682	adsorbent dosage (0.05-0.25 g)	667:696	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
34508964	4	66	theme	experimental	632:643	arg1	conditions					645:654	The optimum experimental conditions	620:654	The optimum experimental conditions	620:654	The optimum experimental conditions, including adsorbent dosage (0.05-0.25 g), contact time (5-25 min), and concentration of malathion (5-25 mg L-1) at five levels were studied using the composite central design (CCD) based on the response surface methodology (RSM).
32330498	0	0	theme	Ficus	89:93	arg1	tree					105:108	Ficus religiosa tree	89:108	Ficus religiosa tree	89:108	Characterization of surface-modified natural cellulosic fiber extracted from the root of Ficus religiosa tree.
32330498	4	1	theme	analysis	626:633	arg1	results					635:641	The thermogravimetric analysis results	604:641	The thermogravimetric analysis results	604:641	The thermogravimetric analysis results reveal that the alkali-treated FRRF soaked for 60 min holds a maximum range of thermal stability (improved by 9.54%); in turn, the remaining analyses have been carried out with that fiber samples.
32330498	4	2	theme	remaining	774:782	arg1	analyses					784:791	the remaining analyses	770:791	the remaining analyses	770:791	The thermogravimetric analysis results reveal that the alkali-treated FRRF soaked for 60 min holds a maximum range of thermal stability (improved by 9.54%); in turn, the remaining analyses have been carried out with that fiber samples.
32330498	7	3	theme	FRRF	1097:1100	arg1	surface					1074:1080	the surface	1070:1080	the surface of the treated FRRF	1070:1100	The morphology study results ensured that the surface of the treated FRRF became so rough comparably, which confirms the removal of unwanted wax and impurities on the fiber surface.
32330498	1	4	theme	better	222:227	arg1	reinforcement					229:241	the better reinforcement	218:241	the better reinforcement for the natural fiber-reinforced composite structures	218:295	The foremost intention of this work is to test the suitability of the Ficus religiosa Root Fiber (FRRF) as the better reinforcement for the natural fiber-reinforced composite structures.
32330498	0	5	theme	tree	105:108	arg1	root					81:84	the root	77:84	the root of Ficus religiosa tree	77:108	Characterization of surface-modified natural cellulosic fiber extracted from the root of Ficus religiosa tree.
32330498	4	6	theme	maximum	705:711	arg1	range					713:717	a maximum range	703:717	a maximum range of thermal stability	703:738	The thermogravimetric analysis results reveal that the alkali-treated FRRF soaked for 60 min holds a maximum range of thermal stability (improved by 9.54%); in turn, the remaining analyses have been carried out with that fiber samples.
32330498	5	7	theme	cellulose	866:874	arg1	contents					876:883	cellulose contents	866:883	cellulose contents	866:883	The increased quantity of cellulose contents was witnessed over the surface of treated FRRF.
32330498	0	8	theme	religiosa	95:103	arg1	tree					105:108	Ficus religiosa tree	89:108	Ficus religiosa tree	89:108	Characterization of surface-modified natural cellulosic fiber extracted from the root of Ficus religiosa tree.
32330498	7	9	theme	morphology	1032:1041	arg1	results					1049:1055	The morphology study results	1028:1055	The morphology study results	1028:1055	The morphology study results ensured that the surface of the treated FRRF became so rough comparably, which confirms the removal of unwanted wax and impurities on the fiber surface.
32330498	5	10	theme	contents	876:883	arg1	quantity					854:861	The increased quantity	840:861	The increased quantity of cellulose contents	840:883	The increased quantity of cellulose contents was witnessed over the surface of treated FRRF.
32330498	4	11	theme	alkali-treated	659:672	arg1	FRRF					674:677	the alkali-treated FRRF	655:677	the alkali-treated FRRF soaked for 60 min	655:695	The thermogravimetric analysis results reveal that the alkali-treated FRRF soaked for 60 min holds a maximum range of thermal stability (improved by 9.54%); in turn, the remaining analyses have been carried out with that fiber samples.
32330498	4	12	dep	holds	697:701	arg1	improved					741:748	improved	741:748	improved by 9.54%	741:757	The thermogravimetric analysis results reveal that the alkali-treated FRRF soaked for 60 min holds a maximum range of thermal stability (improved by 9.54%); in turn, the remaining analyses have been carried out with that fiber samples.
32330498	6	13	from	%	975:975	arg1	improvement					937:947	The improvement	933:947	The improvement in the CI from 42.92-48.64%	933:975	The improvement in the CI from 42.92-48.64% was noted as the result of X-Ray Diffraction test.
32330498	6	13	from	%	975:975	arg1	CI					956:957	the CI	952:957	the CI from 42.92-48.64%	952:975	The improvement in the CI from 42.92-48.64% was noted as the result of X-Ray Diffraction test.
32330498	6	13	from	%	975:975	arg1	result					994:999	the result	990:999	the result of X-Ray Diffraction test	990:1025	The improvement in the CI from 42.92-48.64% was noted as the result of X-Ray Diffraction test.
32330498	8	14	theme	alkali	1337:1342	arg1	treatment					1344:1352	the optimal alkali treatment	1325:1352	the optimal alkali treatment	1325:1352	All the above observations validated that the proposed fiber is suitable to prepare the composite structures after the optimal alkali treatment.
32330498	3	15	from	fiber	544:548	arg1	solution					564:571	the alkali solution	553:571	the alkali solution	553:571	Five samples of FRRF have been prepared by soaking the raw fiber in the alkali solution under different soaking times.
32330498	2	16	theme	5 wt	461:464	arg1	%					465:465	5 wt%	461:465	5 wt% of NaOH solution	461:482	In the current work, the attempts have been made with the view of improving the physical, thermal, chemical, surface, and crystalline properties of FRRF employing 5 wt% of NaOH solution.
32330498	2	16	theme	5 wt	461:464	arg1	solution					475:482	NaOH solution	470:482	NaOH solution	470:482	In the current work, the attempts have been made with the view of improving the physical, thermal, chemical, surface, and crystalline properties of FRRF employing 5 wt% of NaOH solution.
32330498	0	17	theme	fiber	56:60	arg1	Characterization					0:15	Characterization	0:15	Characterization of surface-modified natural cellulosic fiber	0:60	Characterization of surface-modified natural cellulosic fiber extracted from the root of Ficus religiosa tree.
32330498	8	18	theme	above	1218:1222	arg1	observations					1224:1235	All the above observations	1210:1235	All the above observations	1210:1235	All the above observations validated that the proposed fiber is suitable to prepare the composite structures after the optimal alkali treatment.
32330498	7	19	theme	wax	1169:1171	arg1	removal					1149:1155	the removal	1145:1155	the removal of unwanted wax	1145:1171	The morphology study results ensured that the surface of the treated FRRF became so rough comparably, which confirms the removal of unwanted wax and impurities on the fiber surface.
32330498	6	20	from	improvement	937:947	arg1	CI					956:957	the CI	952:957	the CI from 42.92-48.64%	952:975	The improvement in the CI from 42.92-48.64% was noted as the result of X-Ray Diffraction test.
32330498	6	20	from	improvement	937:947	arg1	%					975:975	42.92-48.64%	964:975	42.92-48.64%	964:975	The improvement in the CI from 42.92-48.64% was noted as the result of X-Ray Diffraction test.
32330498	7	21	theme	study	1043:1047	arg1	results					1049:1055	The morphology study results	1028:1055	The morphology study results	1028:1055	The morphology study results ensured that the surface of the treated FRRF became so rough comparably, which confirms the removal of unwanted wax and impurities on the fiber surface.
32330498	5	22	theme	increased	844:852	arg1	quantity					854:861	The increased quantity	840:861	The increased quantity of cellulose contents	840:883	The increased quantity of cellulose contents was witnessed over the surface of treated FRRF.
32330498	7	23	theme	fiber	1195:1199	arg1	surface					1201:1207	the fiber surface	1191:1207	the fiber surface	1191:1207	The morphology study results ensured that the surface of the treated FRRF became so rough comparably, which confirms the removal of unwanted wax and impurities on the fiber surface.
32330498	0	24	theme	natural	37:43	arg1	fiber					56:60	surface-modified natural cellulosic fiber	20:60	surface-modified natural cellulosic fiber	20:60	Characterization of surface-modified natural cellulosic fiber extracted from the root of Ficus religiosa tree.
32330498	1	25	theme	natural	251:257	arg1	structures					286:295	the natural fiber-reinforced composite structures	247:295	the natural fiber-reinforced composite structures	247:295	The foremost intention of this work is to test the suitability of the Ficus religiosa Root Fiber (FRRF) as the better reinforcement for the natural fiber-reinforced composite structures.
32330498	4	26	theme	fiber	825:829	arg1	samples					831:837	that fiber samples	820:837	that fiber samples	820:837	The thermogravimetric analysis results reveal that the alkali-treated FRRF soaked for 60 min holds a maximum range of thermal stability (improved by 9.54%); in turn, the remaining analyses have been carried out with that fiber samples.
32330498	2	27	dep	the	319:321	arg1	attempts					323:330	attempts	323:330	attempts	323:330	In the current work, the attempts have been made with the view of improving the physical, thermal, chemical, surface, and crystalline properties of FRRF employing 5 wt% of NaOH solution.
32330498	1	28	theme	Ficus	181:185	arg1	FRRF					209:212	FRRF	209:212	FRRF	209:212	The foremost intention of this work is to test the suitability of the Ficus religiosa Root Fiber (FRRF) as the better reinforcement for the natural fiber-reinforced composite structures.
32330498	1	28	theme	Ficus	181:185	arg1	Fiber					202:206	the Ficus religiosa Root Fiber	177:206	the Ficus religiosa Root Fiber (FRRF)	177:213	The foremost intention of this work is to test the suitability of the Ficus religiosa Root Fiber (FRRF) as the better reinforcement for the natural fiber-reinforced composite structures.
32330498	0	29	theme	surface-modified	20:35	arg1	fiber					56:60	surface-modified natural cellulosic fiber	20:60	surface-modified natural cellulosic fiber	20:60	Characterization of surface-modified natural cellulosic fiber extracted from the root of Ficus religiosa tree.
32330498	1	30	theme	fiber-reinforced	259:274	arg1	structures					286:295	the natural fiber-reinforced composite structures	247:295	the natural fiber-reinforced composite structures	247:295	The foremost intention of this work is to test the suitability of the Ficus religiosa Root Fiber (FRRF) as the better reinforcement for the natural fiber-reinforced composite structures.
32330498	3	31	theme	FRRF	501:504	arg1	samples					490:496	Five samples	485:496	Five samples of FRRF	485:504	Five samples of FRRF have been prepared by soaking the raw fiber in the alkali solution under different soaking times.
32330498	2	32	theme	physical	378:385	arg1	surface					407:413	the physical, thermal, chemical, surface, and crystalline properties	374:441	surface	407:413	In the current work, the attempts have been made with the view of improving the physical, thermal, chemical, surface, and crystalline properties of FRRF employing 5 wt% of NaOH solution.
32330498	2	33	theme	crystalline	420:430	arg1	properties					432:441	the physical, thermal, chemical, surface, and crystalline properties	374:441	properties	432:441	In the current work, the attempts have been made with the view of improving the physical, thermal, chemical, surface, and crystalline properties of FRRF employing 5 wt% of NaOH solution.
32330498	1	34	theme	composite	276:284	arg1	structures					286:295	the natural fiber-reinforced composite structures	247:295	the natural fiber-reinforced composite structures	247:295	The foremost intention of this work is to test the suitability of the Ficus religiosa Root Fiber (FRRF) as the better reinforcement for the natural fiber-reinforced composite structures.
32330498	3	35	theme	different	579:587	arg1	times					597:601	different soaking times	579:601	different soaking times	579:601	Five samples of FRRF have been prepared by soaking the raw fiber in the alkali solution under different soaking times.
32330498	5	36	theme	treated	919:925	arg1	FRRF					927:930	treated FRRF	919:930	treated FRRF	919:930	The increased quantity of cellulose contents was witnessed over the surface of treated FRRF.
32330498	1	37	theme	Root	197:200	arg1	FRRF					209:212	FRRF	209:212	FRRF	209:212	The foremost intention of this work is to test the suitability of the Ficus religiosa Root Fiber (FRRF) as the better reinforcement for the natural fiber-reinforced composite structures.
32330498	1	37	theme	Root	197:200	arg1	Fiber					202:206	the Ficus religiosa Root Fiber	177:206	the Ficus religiosa Root Fiber (FRRF)	177:213	The foremost intention of this work is to test the suitability of the Ficus religiosa Root Fiber (FRRF) as the better reinforcement for the natural fiber-reinforced composite structures.
32330498	0	38	theme	cellulosic	45:54	arg1	fiber					56:60	surface-modified natural cellulosic fiber	20:60	surface-modified natural cellulosic fiber	20:60	Characterization of surface-modified natural cellulosic fiber extracted from the root of Ficus religiosa tree.
32330498	2	39	theme	solution	475:482	arg1	%					465:465	5 wt%	461:465	5 wt% of NaOH solution	461:482	In the current work, the attempts have been made with the view of improving the physical, thermal, chemical, surface, and crystalline properties of FRRF employing 5 wt% of NaOH solution.
32330498	2	39	theme	solution	475:482	arg1	solution					475:482	NaOH solution	470:482	NaOH solution	470:482	In the current work, the attempts have been made with the view of improving the physical, thermal, chemical, surface, and crystalline properties of FRRF employing 5 wt% of NaOH solution.
32330498	3	40	theme	alkali	557:562	arg1	solution					564:571	the alkali solution	553:571	the alkali solution	553:571	Five samples of FRRF have been prepared by soaking the raw fiber in the alkali solution under different soaking times.
32330498	5	41	theme	FRRF	927:930	arg1	surface					908:914	the surface	904:914	the surface of treated FRRF	904:930	The increased quantity of cellulose contents was witnessed over the surface of treated FRRF.
32330498	1	42	theme	Fiber	202:206	arg1	suitability					162:172	the suitability	158:172	the suitability of the Ficus religiosa Root Fiber (FRRF) as the better reinforcement for the natural fiber-reinforced composite structures	158:295	The foremost intention of this work is to test the suitability of the Ficus religiosa Root Fiber (FRRF) as the better reinforcement for the natural fiber-reinforced composite structures.
32330498	2	43	theme	NaOH	470:473	arg1	solution					475:482	NaOH solution	470:482	NaOH solution	470:482	In the current work, the attempts have been made with the view of improving the physical, thermal, chemical, surface, and crystalline properties of FRRF employing 5 wt% of NaOH solution.
32330498	8	44	theme	optimal	1329:1335	arg1	treatment					1344:1352	the optimal alkali treatment	1325:1352	the optimal alkali treatment	1325:1352	All the above observations validated that the proposed fiber is suitable to prepare the composite structures after the optimal alkali treatment.
32330498	1	45	theme	foremost	115:122	arg1	intention					124:132	The foremost intention	111:132	The foremost intention of this work	111:145	The foremost intention of this work is to test the suitability of the Ficus religiosa Root Fiber (FRRF) as the better reinforcement for the natural fiber-reinforced composite structures.
32330498	2	46	dep	physical	378:385	arg1	chemical					397:404	chemical	397:404	chemical	397:404	In the current work, the attempts have been made with the view of improving the physical, thermal, chemical, surface, and crystalline properties of FRRF employing 5 wt% of NaOH solution.
32330498	2	46	dep	physical	378:385	arg1	thermal					388:394	thermal	388:394	thermal	388:394	In the current work, the attempts have been made with the view of improving the physical, thermal, chemical, surface, and crystalline properties of FRRF employing 5 wt% of NaOH solution.
32330498	6	47	theme	Diffraction	1010:1020	arg1	test					1022:1025	X-Ray Diffraction test	1004:1025	X-Ray Diffraction test	1004:1025	The improvement in the CI from 42.92-48.64% was noted as the result of X-Ray Diffraction test.
32330498	8	48	theme	composite	1298:1306	arg1	structures					1308:1317	the composite structures	1294:1317	the composite structures	1294:1317	All the above observations validated that the proposed fiber is suitable to prepare the composite structures after the optimal alkali treatment.
32330498	4	49	theme	stability	730:738	arg1	range					713:717	a maximum range	703:717	a maximum range of thermal stability	703:738	The thermogravimetric analysis results reveal that the alkali-treated FRRF soaked for 60 min holds a maximum range of thermal stability (improved by 9.54%); in turn, the remaining analyses have been carried out with that fiber samples.
32330498	6	50	theme	X-Ray	1004:1008	arg1	test					1022:1025	X-Ray Diffraction test	1004:1025	X-Ray Diffraction test	1004:1025	The improvement in the CI from 42.92-48.64% was noted as the result of X-Ray Diffraction test.
32330498	3	51	theme	raw	540:542	arg1	fiber					544:548	the raw fiber	536:548	the raw fiber in the alkali solution	536:571	Five samples of FRRF have been prepared by soaking the raw fiber in the alkali solution under different soaking times.
32330498	3	52	theme	soaking	589:595	arg1	times					597:601	different soaking times	579:601	different soaking times	579:601	Five samples of FRRF have been prepared by soaking the raw fiber in the alkali solution under different soaking times.
32330498	4	53	theme	thermal	722:728	arg1	stability					730:738	thermal stability	722:738	thermal stability	722:738	The thermogravimetric analysis results reveal that the alkali-treated FRRF soaked for 60 min holds a maximum range of thermal stability (improved by 9.54%); in turn, the remaining analyses have been carried out with that fiber samples.
32330498	2	54	theme	current	305:311	arg1	work					313:316	the current work	301:316	the current work	301:316	In the current work, the attempts have been made with the view of improving the physical, thermal, chemical, surface, and crystalline properties of FRRF employing 5 wt% of NaOH solution.
32330498	6	55	theme	test	1022:1025	arg1	improvement					937:947	The improvement	933:947	The improvement in the CI from 42.92-48.64%	933:975	The improvement in the CI from 42.92-48.64% was noted as the result of X-Ray Diffraction test.
32330498	6	55	theme	test	1022:1025	arg1	result					994:999	the result	990:999	the result of X-Ray Diffraction test	990:1025	The improvement in the CI from 42.92-48.64% was noted as the result of X-Ray Diffraction test.
32330498	8	56	theme	proposed	1256:1263	arg1	suitable					1274:1281	suitable	1274:1281	suitable	1274:1281	All the above observations validated that the proposed fiber is suitable to prepare the composite structures after the optimal alkali treatment.
32330498	8	56	theme	proposed	1256:1263	arg1	fiber					1265:1269	the proposed fiber	1252:1269	the proposed fiber	1252:1269	All the above observations validated that the proposed fiber is suitable to prepare the composite structures after the optimal alkali treatment.
32330498	1	57	dep	Ficus	181:185	arg1	religiosa					187:195	religiosa	187:195	religiosa	187:195	The foremost intention of this work is to test the suitability of the Ficus religiosa Root Fiber (FRRF) as the better reinforcement for the natural fiber-reinforced composite structures.
32330498	1	58	theme	work	142:145	arg1	intention					124:132	The foremost intention	111:132	The foremost intention of this work	111:145	The foremost intention of this work is to test the suitability of the Ficus religiosa Root Fiber (FRRF) as the better reinforcement for the natural fiber-reinforced composite structures.
32330498	2	59	theme	FRRF	446:449	arg1	properties					432:441	the physical, thermal, chemical, surface, and crystalline properties	374:441	properties	432:441	In the current work, the attempts have been made with the view of improving the physical, thermal, chemical, surface, and crystalline properties of FRRF employing 5 wt% of NaOH solution.
32330498	2	59	theme	FRRF	446:449	arg1	surface					407:413	the physical, thermal, chemical, surface, and crystalline properties	374:441	surface	407:413	In the current work, the attempts have been made with the view of improving the physical, thermal, chemical, surface, and crystalline properties of FRRF employing 5 wt% of NaOH solution.
32330498	7	60	theme	unwanted	1160:1167	arg1	wax					1169:1171	unwanted wax	1160:1171	unwanted wax	1160:1171	The morphology study results ensured that the surface of the treated FRRF became so rough comparably, which confirms the removal of unwanted wax and impurities on the fiber surface.
32330498	4	61	theme	thermogravimetric	608:624	arg1	results					635:641	The thermogravimetric analysis results	604:641	The thermogravimetric analysis results	604:641	The thermogravimetric analysis results reveal that the alkali-treated FRRF soaked for 60 min holds a maximum range of thermal stability (improved by 9.54%); in turn, the remaining analyses have been carried out with that fiber samples.
32330498	7	62	theme	treated	1089:1095	arg1	FRRF					1097:1100	the treated FRRF	1085:1100	the treated FRRF	1085:1100	The morphology study results ensured that the surface of the treated FRRF became so rough comparably, which confirms the removal of unwanted wax and impurities on the fiber surface.
32414437	0	0	theme	traumatic	98:106	arg1	hemorrhage					108:117	traumatic hemorrhage	98:117	traumatic hemorrhage	98:117	Cytotoxicity and hemostatic activity of chitosan/carrageenan composite wound healing dressing for traumatic hemorrhage.
32414437	5	1	theme	enhanced	697:704	arg1	attachment					711:720	enhanced cell attachment	697:720	enhanced cell attachment	697:720	Further, human dermal fibroblast cells were seeded on dressing and demonstrated non-toxic effects on the cells by showing enhanced cell attachment and proliferation.
32414437	5	2	theme	human	584:588	arg1	cells					608:612	human dermal fibroblast cells	584:612	human dermal fibroblast cells	584:612	Further, human dermal fibroblast cells were seeded on dressing and demonstrated non-toxic effects on the cells by showing enhanced cell attachment and proliferation.
32414437	5	3	theme	cell	706:709	arg1	attachment					711:720	enhanced cell attachment	697:720	enhanced cell attachment	697:720	Further, human dermal fibroblast cells were seeded on dressing and demonstrated non-toxic effects on the cells by showing enhanced cell attachment and proliferation.
32414437	5	4	theme	non-toxic	655:663	arg1	effects					665:671	non-toxic effects	655:671	non-toxic effects on the cells	655:684	Further, human dermal fibroblast cells were seeded on dressing and demonstrated non-toxic effects on the cells by showing enhanced cell attachment and proliferation.
32414437	6	5	theme	dressing	779:786	arg1	properties					761:770	In vitro hemostatic properties	741:770	In vitro hemostatic properties of the dressing	741:786	In vitro hemostatic properties of the dressing were analyzed by human Thrombin-Antithrombin assay.
32414437	8	6	theme	composite	1055:1063	arg1	combination					1090:1100	a potent combination	1081:1100	a potent combination to accelerate hemostatic activity against hemorrhage and promote tissue growth for effective wound healing	1081:1207	Thus, it is concluded that the composite dressing can be a potent combination to accelerate hemostatic activity against hemorrhage and promote tissue growth for effective wound healing.
32414437	8	6	theme	composite	1055:1063	arg1	dressing					1065:1072	the composite dressing	1051:1072	the composite dressing	1051:1072	Thus, it is concluded that the composite dressing can be a potent combination to accelerate hemostatic activity against hemorrhage and promote tissue growth for effective wound healing.
32414437	3	7	theme	promising	374:382	arg1	material					384:391	a promising material	372:391	a promising material	372:391	This dressing was fabricated using freeze-drying that will serve as a promising material to promote hemostasis and tissue growth required during hemorrhage.
32414437	3	7	theme	promising	374:382	arg1	freeze-drying					339:351	freeze-drying	339:351	freeze-drying that will serve as a promising material to promote hemostasis and tissue growth required during hemorrhage	339:458	This dressing was fabricated using freeze-drying that will serve as a promising material to promote hemostasis and tissue growth required during hemorrhage.
32414437	4	8	theme	physiochemical	508:521	arg1	analysis					523:530	its physiochemical analysis	504:530	its physiochemical analysis	504:530	The efficacy of dressing was evaluated for its physiochemical analysis, surface morphology, and biodegradability.
32414437	6	9	dep	In	741:742	arg1	vitro					744:748	vitro	744:748	vitro	744:748	In vitro hemostatic properties of the dressing were analyzed by human Thrombin-Antithrombin assay.
32414437	4	10	theme	surface	533:539	arg1	morphology					541:550	surface morphology	533:550	surface morphology	533:550	The efficacy of dressing was evaluated for its physiochemical analysis, surface morphology, and biodegradability.
32414437	8	11	theme	hemostatic	1116:1125	arg1	activity					1127:1134	hemostatic activity	1116:1134	hemostatic activity against hemorrhage	1116:1153	Thus, it is concluded that the composite dressing can be a potent combination to accelerate hemostatic activity against hemorrhage and promote tissue growth for effective wound healing.
32414437	7	12	theme	platelet	921:928	arg1	adhesion					930:937	platelet adhesion	921:937	platelet adhesion	921:937	The dressing formed showed steady blood coagulation implying red blood cells and platelet adhesion that helped in thrombin formation, which is responsible for enhancing wound healing.
32414437	1	13	theme	combat	186:191	arg1	fields					193:198	combat fields	186:198	combat fields	186:198	Hemorrhage remains a big threat to trauma patients, especially in combat fields.
32414437	3	14	theme	tissue	419:424	arg1	growth					426:431	tissue growth	419:431	tissue growth	419:431	This dressing was fabricated using freeze-drying that will serve as a promising material to promote hemostasis and tissue growth required during hemorrhage.
32414437	6	15	theme	hemostatic	750:759	arg1	properties					761:770	In vitro hemostatic properties	741:770	In vitro hemostatic properties of the dressing	741:786	In vitro hemostatic properties of the dressing were analyzed by human Thrombin-Antithrombin assay.
32414437	8	16	theme	potent	1083:1088	arg1	combination					1090:1100	a potent combination	1081:1100	a potent combination to accelerate hemostatic activity against hemorrhage and promote tissue growth for effective wound healing	1081:1207	Thus, it is concluded that the composite dressing can be a potent combination to accelerate hemostatic activity against hemorrhage and promote tissue growth for effective wound healing.
32414437	8	16	theme	potent	1083:1088	arg1	dressing					1065:1072	the composite dressing	1051:1072	the composite dressing	1051:1072	Thus, it is concluded that the composite dressing can be a potent combination to accelerate hemostatic activity against hemorrhage and promote tissue growth for effective wound healing.
32414437	7	17	theme	wound	1009:1013	arg1	healing					1015:1021	wound healing	1009:1021	wound healing	1009:1021	The dressing formed showed steady blood coagulation implying red blood cells and platelet adhesion that helped in thrombin formation, which is responsible for enhancing wound healing.
32414437	6	18	theme	In	741:742	arg1	properties					761:770	In vitro hemostatic properties	741:770	In vitro hemostatic properties of the dressing	741:786	In vitro hemostatic properties of the dressing were analyzed by human Thrombin-Antithrombin assay.
32414437	0	19	theme	hemostatic	17:26	arg1	activity					28:35	hemostatic activity	17:35	hemostatic activity	17:35	Cytotoxicity and hemostatic activity of chitosan/carrageenan composite wound healing dressing for traumatic hemorrhage.
32414437	7	20	theme	steady	867:872	arg1	coagulation					880:890	steady blood coagulation	867:890	steady blood coagulation	867:890	The dressing formed showed steady blood coagulation implying red blood cells and platelet adhesion that helped in thrombin formation, which is responsible for enhancing wound healing.
32414437	7	21	theme	red	901:903	arg1	cells					911:915	red blood cells	901:915	red blood cells	901:915	The dressing formed showed steady blood coagulation implying red blood cells and platelet adhesion that helped in thrombin formation, which is responsible for enhancing wound healing.
32414437	5	22	from	effects	665:671	arg1	cells					680:684	the cells	676:684	the cells	676:684	Further, human dermal fibroblast cells were seeded on dressing and demonstrated non-toxic effects on the cells by showing enhanced cell attachment and proliferation.
32414437	0	23	theme	chitosan/carrageenan	40:59	arg1	healing					77:83	chitosan/carrageenan composite wound healing	40:83	chitosan/carrageenan composite wound healing	40:83	Cytotoxicity and hemostatic activity of chitosan/carrageenan composite wound healing dressing for traumatic hemorrhage.
32414437	7	24	theme	blood	874:878	arg1	coagulation					880:890	steady blood coagulation	867:890	steady blood coagulation	867:890	The dressing formed showed steady blood coagulation implying red blood cells and platelet adhesion that helped in thrombin formation, which is responsible for enhancing wound healing.
32414437	7	25	theme	blood	905:909	arg1	cells					911:915	red blood cells	901:915	red blood cells	901:915	The dressing formed showed steady blood coagulation implying red blood cells and platelet adhesion that helped in thrombin formation, which is responsible for enhancing wound healing.
32414437	8	26	theme	tissue	1167:1172	arg1	growth					1174:1179	tissue growth	1167:1179	tissue growth for effective wound healing	1167:1207	Thus, it is concluded that the composite dressing can be a potent combination to accelerate hemostatic activity against hemorrhage and promote tissue growth for effective wound healing.
32414437	5	27	theme	dermal	590:595	arg1	cells					608:612	human dermal fibroblast cells	584:612	human dermal fibroblast cells	584:612	Further, human dermal fibroblast cells were seeded on dressing and demonstrated non-toxic effects on the cells by showing enhanced cell attachment and proliferation.
32414437	0	28	theme	wound	71:75	arg1	healing					77:83	chitosan/carrageenan composite wound healing	40:83	chitosan/carrageenan composite wound healing	40:83	Cytotoxicity and hemostatic activity of chitosan/carrageenan composite wound healing dressing for traumatic hemorrhage.
32414437	8	29	theme	effective	1185:1193	arg1	healing					1201:1207	effective wound healing	1185:1207	effective wound healing	1185:1207	Thus, it is concluded that the composite dressing can be a potent combination to accelerate hemostatic activity against hemorrhage and promote tissue growth for effective wound healing.
32414437	2	30	theme	biocompatible	228:240	arg1	dressing					294:301	a biocompatible and biopolymer based chitosan/carrageenan composite dressing	226:301	a biocompatible and biopolymer based chitosan/carrageenan composite dressing	226:301	Therefore, we formulated a biocompatible and biopolymer based chitosan/carrageenan composite dressing.
32414437	5	31	theme	fibroblast	597:606	arg1	cells					608:612	human dermal fibroblast cells	584:612	human dermal fibroblast cells	584:612	Further, human dermal fibroblast cells were seeded on dressing and demonstrated non-toxic effects on the cells by showing enhanced cell attachment and proliferation.
32414437	0	32	theme	composite	61:69	arg1	healing					77:83	chitosan/carrageenan composite wound healing	40:83	chitosan/carrageenan composite wound healing	40:83	Cytotoxicity and hemostatic activity of chitosan/carrageenan composite wound healing dressing for traumatic hemorrhage.
32414437	6	33	theme	Thrombin-Antithrombin	811:831	arg1	assay					833:837	human Thrombin-Antithrombin assay	805:837	human Thrombin-Antithrombin assay	805:837	In vitro hemostatic properties of the dressing were analyzed by human Thrombin-Antithrombin assay.
32414437	1	34	theme	big	141:143	arg1	threat					145:150	a big threat	139:150	a big threat to trauma patients	139:169	Hemorrhage remains a big threat to trauma patients, especially in combat fields.
32414437	6	35	theme	human	805:809	arg1	assay					833:837	human Thrombin-Antithrombin assay	805:837	human Thrombin-Antithrombin assay	805:837	In vitro hemostatic properties of the dressing were analyzed by human Thrombin-Antithrombin assay.
32414437	8	36	theme	wound	1195:1199	arg1	healing					1201:1207	effective wound healing	1185:1207	effective wound healing	1185:1207	Thus, it is concluded that the composite dressing can be a potent combination to accelerate hemostatic activity against hemorrhage and promote tissue growth for effective wound healing.
32414437	4	37	theme	dressing	477:484	arg1	efficacy					465:472	The efficacy	461:472	The efficacy of dressing	461:484	The efficacy of dressing was evaluated for its physiochemical analysis, surface morphology, and biodegradability.
32414437	2	38	theme	composite	284:292	arg1	dressing					294:301	a biocompatible and biopolymer based chitosan/carrageenan composite dressing	226:301	a biocompatible and biopolymer based chitosan/carrageenan composite dressing	226:301	Therefore, we formulated a biocompatible and biopolymer based chitosan/carrageenan composite dressing.
32414437	7	39	theme	thrombin	954:961	arg1	formation					963:971	thrombin formation	954:971	thrombin formation	954:971	The dressing formed showed steady blood coagulation implying red blood cells and platelet adhesion that helped in thrombin formation, which is responsible for enhancing wound healing.
32414437	2	40	theme	chitosan/carrageenan	263:282	arg1	dressing					294:301	a biocompatible and biopolymer based chitosan/carrageenan composite dressing	226:301	a biocompatible and biopolymer based chitosan/carrageenan composite dressing	226:301	Therefore, we formulated a biocompatible and biopolymer based chitosan/carrageenan composite dressing.
32414437	1	41	theme	trauma	155:160	arg1	patients					162:169	trauma patients	155:169	trauma patients	155:169	Hemorrhage remains a big threat to trauma patients, especially in combat fields.
32414437	0	42	theme	healing	77:83	arg1	Cytotoxicity					0:11	Cytotoxicity	0:11	Cytotoxicity	0:11	Cytotoxicity and hemostatic activity of chitosan/carrageenan composite wound healing dressing for traumatic hemorrhage.
32414437	0	42	theme	healing	77:83	arg1	activity					28:35	hemostatic activity	17:35	hemostatic activity	17:35	Cytotoxicity and hemostatic activity of chitosan/carrageenan composite wound healing dressing for traumatic hemorrhage.
32414437	2	43	theme	based	257:261	arg1	dressing					294:301	a biocompatible and biopolymer based chitosan/carrageenan composite dressing	226:301	a biocompatible and biopolymer based chitosan/carrageenan composite dressing	226:301	Therefore, we formulated a biocompatible and biopolymer based chitosan/carrageenan composite dressing.
34048929	9	0	theme	dissolution	1797:1807	arg1	models					1809:1814	mechanically bio-relevant dissolution models	1771:1814	mechanically bio-relevant dissolution models	1771:1814	A considerably higher release rate was also observed from tablets with the weakly basic drugs dipyridamole and propranolol hydrochloride containing pH modifier in case of mechanically bio-relevant dissolution models compared to the USP2 apparatus.
34048929	8	1	theme	GIT	1518:1520	arg1	transit					1522:1528	GIT transit	1518:1528	GIT transit of the mechanically sensitive HPMC tablets with poorly soluble drugs	1518:1597	Our study demonstrates a significantly higher dissolution rate due to mechanical stress during the bio-relevant simulation of GIT transit of the mechanically sensitive HPMC tablets with poorly soluble drugs.
34048929	8	2	theme	soluble	1585:1591	arg1	drugs					1593:1597	poorly soluble drugs	1578:1597	poorly soluble drugs	1578:1597	Our study demonstrates a significantly higher dissolution rate due to mechanical stress during the bio-relevant simulation of GIT transit of the mechanically sensitive HPMC tablets with poorly soluble drugs.
34048929	7	3	theme	orange	1359:1364	arg1	incorporation					1315:1327	the incorporation	1311:1327	the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets	1311:1389	Micro-environmental pH (pHM) was evaluated, using two methods: the cryostatic method with a surface pH electrode, and with the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets.
34048929	4	4	theme	gradient	820:827	arg1	occurrence					801:810	the occurrence	797:810	the occurrence of a pH gradient in the gel layer of the HPMC tablets	797:864	In our study, the occurrence of a pH gradient in the gel layer of the HPMC tablets was observed during simulation of their passage along different compartments of the GIT.
34048929	3	5	theme	research	670:677	arg1	preparation					692:702	the preparation	688:702	the preparation of sustained-release matrix systems	688:738	Moreover, the aim of our research was also the preparation of sustained-release matrix systems with improved - enhanced drug dissolution.
34048929	3	5	theme	research	670:677	arg1	aim					659:661	the aim	655:661	the aim of our research	655:677	Moreover, the aim of our research was also the preparation of sustained-release matrix systems with improved - enhanced drug dissolution.
34048929	9	6	dep	dipyridamole	1694:1705	arg1	hydrochloride					1723:1735	hydrochloride	1723:1735	hydrochloride	1723:1735	A considerably higher release rate was also observed from tablets with the weakly basic drugs dipyridamole and propranolol hydrochloride containing pH modifier in case of mechanically bio-relevant dissolution models compared to the USP2 apparatus.
34048929	6	7	theme	release	1170:1176	arg1	kinetics					1178:1185	the drug release kinetics	1161:1185	the drug release kinetics	1161:1185	Both dissolution methods were also used to evaluate the influence of the mechanical stress on the drug release kinetics.
34048929	2	8	theme	study	250:254	arg1	aim					238:240	The aim	234:240	The aim of this study	234:254	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	1	9	theme	drugs	161:165	arg1	dissolution					146:156	the dissolution	142:156	the dissolution of drugs with pH-dependent solubility	142:194	A commonly used approach to enhance the dissolution of drugs with pH-dependent solubility is the incorporation of pH modifiers.
34048929	7	10	theme	sensitive	1337:1345	arg1	orange					1359:1364	a pH sensitive dye (methyl orange)	1332:1365	a pH sensitive dye (methyl orange)	1332:1365	Micro-environmental pH (pHM) was evaluated, using two methods: the cryostatic method with a surface pH electrode, and with the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets.
34048929	1	11	theme	pH-dependent	172:183	arg1	solubility					185:194	pH-dependent solubility	172:194	pH-dependent solubility	172:194	A commonly used approach to enhance the dissolution of drugs with pH-dependent solubility is the incorporation of pH modifiers.
34048929	6	12	used	used	1102:1105	arg2	methods					1084:1090	Both dissolution methods	1067:1090	Both dissolution methods	1067:1090	Both dissolution methods were also used to evaluate the influence of the mechanical stress on the drug release kinetics.
34048929	4	13	theme	tablets	858:864	arg1	layer					840:844	the gel layer	832:844	the gel layer of the HPMC tablets	832:864	In our study, the occurrence of a pH gradient in the gel layer of the HPMC tablets was observed during simulation of their passage along different compartments of the GIT.
34048929	7	14	theme	surface	1280:1286	arg1	electrode					1291:1299	a surface pH electrode	1278:1299	a surface pH electrode	1278:1299	Micro-environmental pH (pHM) was evaluated, using two methods: the cryostatic method with a surface pH electrode, and with the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets.
34048929	3	15	theme	systems	732:738	arg1	preparation					692:702	the preparation	688:702	the preparation of sustained-release matrix systems	688:738	Moreover, the aim of our research was also the preparation of sustained-release matrix systems with improved - enhanced drug dissolution.
34048929	3	15	theme	systems	732:738	arg1	aim					659:661	the aim	655:661	the aim of our research	655:677	Moreover, the aim of our research was also the preparation of sustained-release matrix systems with improved - enhanced drug dissolution.
34048929	9	16	theme	higher	1615:1620	arg1	rate					1630:1633	A considerably higher release rate	1600:1633	A considerably higher release rate	1600:1633	A considerably higher release rate was also observed from tablets with the weakly basic drugs dipyridamole and propranolol hydrochloride containing pH modifier in case of mechanically bio-relevant dissolution models compared to the USP2 apparatus.
34048929	6	17	theme	mechanical	1140:1149	arg1	stress					1151:1156	the mechanical stress	1136:1156	the mechanical stress	1136:1156	Both dissolution methods were also used to evaluate the influence of the mechanical stress on the drug release kinetics.
34048929	10	18	theme	pHM	2034:2036	arg1	estimation					2038:2047	a rough pHM estimation	2026:2047	a rough pHM estimation	2026:2047	For the assessment of the pHM, the incorporation of a pH indicator dye in the HPMC tablet proved to be more suitable, while the cryostatic method was found to be useful only for a rough pHM estimation.
34048929	3	19	theme	sustained-release	707:723	arg1	systems					732:738	sustained-release matrix systems	707:738	sustained-release matrix systems	707:738	Moreover, the aim of our research was also the preparation of sustained-release matrix systems with improved - enhanced drug dissolution.
34048929	2	20	theme	gastric	549:555	arg1	AGS					568:570	AGS	568:570	AGS	568:570	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	2	20	theme	gastric	549:555	arg1	simulator					557:565	the Advanced gastric simulator	536:565	the Advanced gastric simulator (AGS)	536:571	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	7	21	theme	cryostatic	1255:1264	arg1	method					1266:1271	the cryostatic method	1251:1271	two methods: the cryostatic method with a surface pH electrode, and with the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets	1238:1389	Micro-environmental pH (pHM) was evaluated, using two methods: the cryostatic method with a surface pH electrode, and with the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets.
34048929	7	21	theme	cryostatic	1255:1264	arg1	methods					1242:1248	two methods	1238:1248	two methods: the cryostatic method with a surface pH electrode, and with the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets	1238:1389	Micro-environmental pH (pHM) was evaluated, using two methods: the cryostatic method with a surface pH electrode, and with the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets.
34048929	3	22	theme	enhanced	756:763	arg1	dissolution					770:780	improved - enhanced drug dissolution	745:780	improved - enhanced drug dissolution	745:780	Moreover, the aim of our research was also the preparation of sustained-release matrix systems with improved - enhanced drug dissolution.
34048929	9	23	with	tablets	1658:1664	arg1	propranolol					1711:1721	propranolol	1711:1721	propranolol	1711:1721	A considerably higher release rate was also observed from tablets with the weakly basic drugs dipyridamole and propranolol hydrochloride containing pH modifier in case of mechanically bio-relevant dissolution models compared to the USP2 apparatus.
34048929	9	23	with	tablets	1658:1664	arg1	dipyridamole					1694:1705	the weakly basic drugs dipyridamole	1671:1705	the weakly basic drugs dipyridamole	1671:1705	A considerably higher release rate was also observed from tablets with the weakly basic drugs dipyridamole and propranolol hydrochloride containing pH modifier in case of mechanically bio-relevant dissolution models compared to the USP2 apparatus.
34048929	4	24	located	observed	870:877	arg1	study					790:794	our study	786:794	our study	786:794	In our study, the occurrence of a pH gradient in the gel layer of the HPMC tablets was observed during simulation of their passage along different compartments of the GIT.
34048929	4	24	located	observed	870:877	arg2	occurrence					801:810	the occurrence	797:810	the occurrence of a pH gradient in the gel layer of the HPMC tablets	797:864	In our study, the occurrence of a pH gradient in the gel layer of the HPMC tablets was observed during simulation of their passage along different compartments of the GIT.
34048929	3	25	theme	improved	745:752	arg1	dissolution					770:780	improved - enhanced drug dissolution	745:780	improved - enhanced drug dissolution	745:780	Moreover, the aim of our research was also the preparation of sustained-release matrix systems with improved - enhanced drug dissolution.
34048929	0	26	theme	HPMC	68:71	arg1	tablets					80:86	micro-environmental pH inside HPMC matrix tablets	38:86	micro-environmental pH inside HPMC matrix tablets	38:86	Effect of gastrointestinal transit on micro-environmental pH inside HPMC matrix tablets - in vitro study.
34048929	2	27	from	duration	276:283	arg1	pH					346:347	the micro-environmental pH	322:347	the micro-environmental pH in HPMC matrix	322:362	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	2	28	theme	static	465:470	arg1	USP2					497:500	USP2	497:500	USP2	497:500	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	2	28	theme	static	465:470	arg1	apparatuses					484:494	static dissolution apparatuses	465:494	static dissolution apparatuses (USP2)	465:501	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	2	28	theme	static	465:470	arg1	simulator					557:565	the Advanced gastric simulator	536:565	the Advanced gastric simulator (AGS)	536:571	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	4	29	theme	gel	836:838	arg1	layer					840:844	the gel layer	832:844	the gel layer of the HPMC tablets	832:864	In our study, the occurrence of a pH gradient in the gel layer of the HPMC tablets was observed during simulation of their passage along different compartments of the GIT.
34048929	7	30	with	method	1266:1271	arg1	incorporation					1315:1327	the incorporation	1311:1327	the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets	1311:1389	Micro-environmental pH (pHM) was evaluated, using two methods: the cryostatic method with a surface pH electrode, and with the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets.
34048929	7	30	with	method	1266:1271	arg1	electrode					1291:1299	a surface pH electrode	1278:1299	a surface pH electrode	1278:1299	Micro-environmental pH (pHM) was evaluated, using two methods: the cryostatic method with a surface pH electrode, and with the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets.
34048929	5	31	theme	surrounding	1048:1058	arg1	media					1060:1064	the surrounding media	1044:1064	the surrounding media	1044:1064	The pH gradient was affected by the media composition and duration of tablet exposure to the surrounding media.
34048929	1	32	with	dissolution	146:156	arg1	solubility					185:194	pH-dependent solubility	172:194	pH-dependent solubility	172:194	A commonly used approach to enhance the dissolution of drugs with pH-dependent solubility is the incorporation of pH modifiers.
34048929	2	33	from	extent	289:294	arg1	pH					346:347	the micro-environmental pH	322:347	the micro-environmental pH in HPMC matrix	322:362	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	0	34	from	Effect	0:5	arg1	tablets					80:86	micro-environmental pH inside HPMC matrix tablets	38:86	micro-environmental pH inside HPMC matrix tablets	38:86	Effect of gastrointestinal transit on micro-environmental pH inside HPMC matrix tablets - in vitro study.
34048929	2	35	theme	action	628:633	arg1	simulation					602:611	simulation	602:611	simulation of peristaltic action	602:633	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	6	36	from	influence	1123:1131	arg1	kinetics					1178:1185	the drug release kinetics	1161:1185	the drug release kinetics	1161:1185	Both dissolution methods were also used to evaluate the influence of the mechanical stress on the drug release kinetics.
34048929	10	37	theme	HPMC	1926:1929	arg1	tablet					1931:1936	the HPMC tablet	1922:1936	the HPMC tablet	1922:1936	For the assessment of the pHM, the incorporation of a pH indicator dye in the HPMC tablet proved to be more suitable, while the cryostatic method was found to be useful only for a rough pHM estimation.
34048929	0	38	theme	in	90:91	arg1	study					99:103	in vitro study	90:103	Effect of gastrointestinal transit on micro-environmental pH inside HPMC matrix tablets - in vitro study.	0:104	Effect of gastrointestinal transit on micro-environmental pH inside HPMC matrix tablets - in vitro study.
34048929	4	39	from	occurrence	801:810	arg1	layer					840:844	the gel layer	832:844	the gel layer of the HPMC tablets	832:864	In our study, the occurrence of a pH gradient in the gel layer of the HPMC tablets was observed during simulation of their passage along different compartments of the GIT.
34048929	2	40	dep	formulation	447:457	arg1	USP2					497:500	USP2	497:500	USP2	497:500	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	2	40	dep	formulation	447:457	arg1	apparatuses					484:494	static dissolution apparatuses	465:494	static dissolution apparatuses (USP2)	465:501	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	2	40	dep	formulation	447:457	arg1	simulator					557:565	the Advanced gastric simulator	536:565	the Advanced gastric simulator (AGS)	536:571	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	2	40	dep	formulation	447:457	arg1	approaches					515:524	dynamic approaches	507:524	dynamic approaches including the Advanced gastric simulator (AGS)	507:571	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	2	40	dep	formulation	447:457	arg1	IMSPA					636:640	IMSPA	636:640	IMSPA	636:640	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	5	41	theme	tablet	1025:1030	arg1	exposure					1032:1039	tablet exposure	1025:1039	tablet exposure to the surrounding media	1025:1064	The pH gradient was affected by the media composition and duration of tablet exposure to the surrounding media.
34048929	0	42	dep	in	90:91	arg1	vitro					93:97	vitro	93:97	vitro	93:97	Effect of gastrointestinal transit on micro-environmental pH inside HPMC matrix tablets - in vitro study.
34048929	9	43	theme	drugs	1688:1692	arg1	dipyridamole					1694:1705	the weakly basic drugs dipyridamole	1671:1705	the weakly basic drugs dipyridamole	1671:1705	A considerably higher release rate was also observed from tablets with the weakly basic drugs dipyridamole and propranolol hydrochloride containing pH modifier in case of mechanically bio-relevant dissolution models compared to the USP2 apparatus.
34048929	2	44	theme	HPMC	352:355	arg1	matrix					357:362	HPMC matrix	352:362	HPMC matrix	352:362	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	3	45	with	preparation	692:702	arg1	dissolution					770:780	improved - enhanced drug dissolution	745:780	improved - enhanced drug dissolution	745:780	Moreover, the aim of our research was also the preparation of sustained-release matrix systems with improved - enhanced drug dissolution.
34048929	2	46	theme	hydrodynamic	413:424	arg1	stress					426:431	hydrodynamic stress	413:431	hydrodynamic stress	413:431	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	0	47	theme	gastrointestinal	10:25	arg1	transit					27:33	gastrointestinal transit	10:33	gastrointestinal transit	10:33	Effect of gastrointestinal transit on micro-environmental pH inside HPMC matrix tablets - in vitro study.
34048929	8	48	theme	dissolution	1438:1448	arg1	rate					1450:1453	a significantly higher dissolution rate	1415:1453	a significantly higher dissolution rate due to mechanical stress	1415:1478	Our study demonstrates a significantly higher dissolution rate due to mechanical stress during the bio-relevant simulation of GIT transit of the mechanically sensitive HPMC tablets with poorly soluble drugs.
34048929	10	49	theme	indicator	1905:1913	arg1	dye					1915:1917	a pH indicator dye	1900:1917	a pH indicator dye	1900:1917	For the assessment of the pHM, the incorporation of a pH indicator dye in the HPMC tablet proved to be more suitable, while the cryostatic method was found to be useful only for a rough pHM estimation.
34048929	2	50	theme	tested	440:445	arg1	formulation					447:457	the tested formulation	436:457	the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA))	436:642	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	2	51	theme	effect	312:317	arg1	duration					276:283	the duration	272:283	the duration	272:283	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	2	51	theme	effect	312:317	arg1	extent					289:294	extent	289:294	extent	289:294	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	0	52	theme	pH	58:59	arg1	tablets					80:86	micro-environmental pH inside HPMC matrix tablets	38:86	micro-environmental pH inside HPMC matrix tablets	38:86	Effect of gastrointestinal transit on micro-environmental pH inside HPMC matrix tablets - in vitro study.
34048929	7	53	dep	methods	1242:1248	arg1	method					1266:1271	the cryostatic method	1251:1271	two methods: the cryostatic method with a surface pH electrode, and with the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets	1238:1389	Micro-environmental pH (pHM) was evaluated, using two methods: the cryostatic method with a surface pH electrode, and with the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets.
34048929	7	53	dep	methods	1242:1248	arg1	methods					1242:1248	two methods	1238:1248	two methods: the cryostatic method with a surface pH electrode, and with the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets	1238:1389	Micro-environmental pH (pHM) was evaluated, using two methods: the cryostatic method with a surface pH electrode, and with the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets.
34048929	8	54	theme	mechanical	1462:1471	arg1	stress					1473:1478	mechanical stress	1462:1478	mechanical stress	1462:1478	Our study demonstrates a significantly higher dissolution rate due to mechanical stress during the bio-relevant simulation of GIT transit of the mechanically sensitive HPMC tablets with poorly soluble drugs.
34048929	2	55	theme	pH	299:300	arg1	effect					312:317	pH modifying effect	299:317	pH modifying effect on the micro-environmental pH in HPMC matrix	299:362	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	7	56	theme	Micro-environmental	1188:1206	arg1	pHM					1212:1214	pHM	1212:1214	pHM	1212:1214	Micro-environmental pH (pHM) was evaluated, using two methods: the cryostatic method with a surface pH electrode, and with the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets.
34048929	7	56	theme	Micro-environmental	1188:1206	arg1	pH					1208:1209	Micro-environmental pH	1188:1209	Micro-environmental pH (pHM)	1188:1215	Micro-environmental pH (pHM) was evaluated, using two methods: the cryostatic method with a surface pH electrode, and with the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets.
34048929	8	57	theme	sensitive	1550:1558	arg1	tablets					1565:1571	the mechanically sensitive HPMC tablets	1533:1571	the mechanically sensitive HPMC tablets with poorly soluble drugs	1533:1597	Our study demonstrates a significantly higher dissolution rate due to mechanical stress during the bio-relevant simulation of GIT transit of the mechanically sensitive HPMC tablets with poorly soluble drugs.
34048929	1	58	theme	modifiers	223:231	arg1	approach					122:129	A commonly used approach	106:129	A commonly used approach to enhance the dissolution of drugs with pH-dependent solubility	106:194	A commonly used approach to enhance the dissolution of drugs with pH-dependent solubility is the incorporation of pH modifiers.
34048929	1	58	theme	modifiers	223:231	arg1	incorporation					203:215	the incorporation	199:215	the incorporation of pH modifiers	199:231	A commonly used approach to enhance the dissolution of drugs with pH-dependent solubility is the incorporation of pH modifiers.
34048929	9	59	theme	pH	1748:1749	arg1	modifier					1751:1758	pH modifier	1748:1758	pH modifier in case of mechanically bio-relevant dissolution models compared to the USP2 apparatus	1748:1845	A considerably higher release rate was also observed from tablets with the weakly basic drugs dipyridamole and propranolol hydrochloride containing pH modifier in case of mechanically bio-relevant dissolution models compared to the USP2 apparatus.
34048929	0	60	dep	Effect	0:5	arg1	study					99:103	in vitro study	90:103	Effect of gastrointestinal transit on micro-environmental pH inside HPMC matrix tablets - in vitro study.	0:104	Effect of gastrointestinal transit on micro-environmental pH inside HPMC matrix tablets - in vitro study.
34048929	10	61	theme	dye	1915:1917	arg1	incorporation					1883:1895	the incorporation	1879:1895	the incorporation of a pH indicator dye in the HPMC tablet	1879:1936	For the assessment of the pHM, the incorporation of a pH indicator dye in the HPMC tablet proved to be more suitable, while the cryostatic method was found to be useful only for a rough pHM estimation.
34048929	10	61	theme	dye	1915:1917	arg1	suitable					1956:1963	suitable	1956:1963	suitable	1956:1963	For the assessment of the pHM, the incorporation of a pH indicator dye in the HPMC tablet proved to be more suitable, while the cryostatic method was found to be useful only for a rough pHM estimation.
34048929	9	62	theme	bio-relevant	1784:1795	arg1	models					1809:1814	mechanically bio-relevant dissolution models	1771:1814	mechanically bio-relevant dissolution models	1771:1814	A considerably higher release rate was also observed from tablets with the weakly basic drugs dipyridamole and propranolol hydrochloride containing pH modifier in case of mechanically bio-relevant dissolution models compared to the USP2 apparatus.
34048929	5	63	dep	media	991:995	arg1	media					991:995	the media composition and duration	987:1020	the media composition and duration of tablet exposure to the surrounding media	987:1064	The pH gradient was affected by the media composition and duration of tablet exposure to the surrounding media.
34048929	5	63	dep	media	991:995	arg1	duration					1013:1020	duration	1013:1020	duration	1013:1020	The pH gradient was affected by the media composition and duration of tablet exposure to the surrounding media.
34048929	5	63	dep	media	991:995	arg1	composition					997:1007	composition	997:1007	composition	997:1007	The pH gradient was affected by the media composition and duration of tablet exposure to the surrounding media.
34048929	8	64	theme	tablets	1565:1571	arg1	transit					1522:1528	GIT transit	1518:1528	GIT transit of the mechanically sensitive HPMC tablets with poorly soluble drugs	1518:1597	Our study demonstrates a significantly higher dissolution rate due to mechanical stress during the bio-relevant simulation of GIT transit of the mechanically sensitive HPMC tablets with poorly soluble drugs.
34048929	7	65	theme	methyl	1352:1357	arg1	orange					1359:1364	a pH sensitive dye (methyl orange)	1332:1365	a pH sensitive dye (methyl orange)	1332:1365	Micro-environmental pH (pHM) was evaluated, using two methods: the cryostatic method with a surface pH electrode, and with the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets.
34048929	7	66	theme	dye	1347:1349	arg1	orange					1359:1364	a pH sensitive dye (methyl orange)	1332:1365	a pH sensitive dye (methyl orange)	1332:1365	Micro-environmental pH (pHM) was evaluated, using two methods: the cryostatic method with a surface pH electrode, and with the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets.
34048929	4	67	theme	pH	817:818	arg1	gradient					820:827	a pH gradient	815:827	a pH gradient	815:827	In our study, the occurrence of a pH gradient in the gel layer of the HPMC tablets was observed during simulation of their passage along different compartments of the GIT.
34048929	2	68	from	effect	312:317	arg1	pH					346:347	the micro-environmental pH	322:347	the micro-environmental pH in HPMC matrix	322:362	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	8	69	theme	transit	1522:1528	arg1	simulation					1504:1513	the bio-relevant simulation	1487:1513	the bio-relevant simulation of GIT transit of the mechanically sensitive HPMC tablets with poorly soluble drugs	1487:1597	Our study demonstrates a significantly higher dissolution rate due to mechanical stress during the bio-relevant simulation of GIT transit of the mechanically sensitive HPMC tablets with poorly soluble drugs.
34048929	7	70	theme	pH	1334:1335	arg1	orange					1359:1364	a pH sensitive dye (methyl orange)	1332:1365	a pH sensitive dye (methyl orange)	1332:1365	Micro-environmental pH (pHM) was evaluated, using two methods: the cryostatic method with a surface pH electrode, and with the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets.
34048929	9	71	theme	USP2	1832:1835	arg1	apparatus					1837:1845	the USP2 apparatus	1828:1845	the USP2 apparatus	1828:1845	A considerably higher release rate was also observed from tablets with the weakly basic drugs dipyridamole and propranolol hydrochloride containing pH modifier in case of mechanically bio-relevant dissolution models compared to the USP2 apparatus.
34048929	6	72	theme	drug	1165:1168	arg1	kinetics					1178:1185	the drug release kinetics	1161:1185	the drug release kinetics	1161:1185	Both dissolution methods were also used to evaluate the influence of the mechanical stress on the drug release kinetics.
34048929	2	73	theme	Intestinal	581:590	arg1	model					592:596	the Intestinal model	577:596	the Intestinal model for simulation of peristaltic action	577:633	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	2	74	from	pH	346:347	arg1	matrix					357:362	HPMC matrix	352:362	HPMC matrix	352:362	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	2	74	from	pH	346:347	arg1	duration					276:283	the duration	272:283	the duration	272:283	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	2	74	from	pH	346:347	arg1	extent					289:294	extent	289:294	extent	289:294	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	6	75	theme	dissolution	1072:1082	arg1	methods					1084:1090	Both dissolution methods	1067:1090	Both dissolution methods	1067:1090	Both dissolution methods were also used to evaluate the influence of the mechanical stress on the drug release kinetics.
34048929	10	76	theme	pHM	1874:1876	arg1	assessment					1856:1865	the assessment	1852:1865	the assessment of the pHM	1852:1876	For the assessment of the pHM, the incorporation of a pH indicator dye in the HPMC tablet proved to be more suitable, while the cryostatic method was found to be useful only for a rough pHM estimation.
34048929	5	77	theme	pH	959:960	arg1	gradient					962:969	The pH gradient	955:969	The pH gradient	955:969	The pH gradient was affected by the media composition and duration of tablet exposure to the surrounding media.
34048929	4	78	theme	HPMC	853:856	arg1	tablets					858:864	the HPMC tablets	849:864	the HPMC tablets	849:864	In our study, the occurrence of a pH gradient in the gel layer of the HPMC tablets was observed during simulation of their passage along different compartments of the GIT.
34048929	7	79	theme	pH	1288:1289	arg1	electrode					1291:1299	a surface pH electrode	1278:1299	a surface pH electrode	1278:1299	Micro-environmental pH (pHM) was evaluated, using two methods: the cryostatic method with a surface pH electrode, and with the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets.
34048929	9	80	theme	release	1622:1628	arg1	rate					1630:1633	A considerably higher release rate	1600:1633	A considerably higher release rate	1600:1633	A considerably higher release rate was also observed from tablets with the weakly basic drugs dipyridamole and propranolol hydrochloride containing pH modifier in case of mechanically bio-relevant dissolution models compared to the USP2 apparatus.
34048929	10	81	theme	rough	2028:2032	arg1	estimation					2038:2047	a rough pHM estimation	2026:2047	a rough pHM estimation	2026:2047	For the assessment of the pHM, the incorporation of a pH indicator dye in the HPMC tablet proved to be more suitable, while the cryostatic method was found to be useful only for a rough pHM estimation.
34048929	3	82	theme	matrix	725:730	arg1	systems					732:738	sustained-release matrix systems	707:738	sustained-release matrix systems	707:738	Moreover, the aim of our research was also the preparation of sustained-release matrix systems with improved - enhanced drug dissolution.
34048929	1	83	theme	used	117:120	arg1	approach					122:129	A commonly used approach	106:129	A commonly used approach to enhance the dissolution of drugs with pH-dependent solubility	106:194	A commonly used approach to enhance the dissolution of drugs with pH-dependent solubility is the incorporation of pH modifiers.
34048929	1	83	theme	used	117:120	arg1	incorporation					203:215	the incorporation	199:215	the incorporation of pH modifiers	199:231	A commonly used approach to enhance the dissolution of drugs with pH-dependent solubility is the incorporation of pH modifiers.
34048929	2	84	theme	Advanced	540:547	arg1	AGS					568:570	AGS	568:570	AGS	568:570	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	2	84	theme	Advanced	540:547	arg1	simulator					557:565	the Advanced gastric simulator	536:565	the Advanced gastric simulator (AGS)	536:571	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	3	85	theme	drug	765:768	arg1	dissolution					770:780	improved - enhanced drug dissolution	745:780	improved - enhanced drug dissolution	745:780	Moreover, the aim of our research was also the preparation of sustained-release matrix systems with improved - enhanced drug dissolution.
34048929	0	86	theme	matrix	73:78	arg1	tablets					80:86	micro-environmental pH inside HPMC matrix tablets	38:86	micro-environmental pH inside HPMC matrix tablets	38:86	Effect of gastrointestinal transit on micro-environmental pH inside HPMC matrix tablets - in vitro study.
34048929	9	87	contain	containing	1737:1746	arg2	modifier					1751:1758	pH modifier	1748:1758	pH modifier in case of mechanically bio-relevant dissolution models compared to the USP2 apparatus	1748:1845	A considerably higher release rate was also observed from tablets with the weakly basic drugs dipyridamole and propranolol hydrochloride containing pH modifier in case of mechanically bio-relevant dissolution models compared to the USP2 apparatus.
34048929	9	87	contain	containing	1737:1746	arg1	dipyridamole					1694:1705	the weakly basic drugs dipyridamole	1671:1705	the weakly basic drugs dipyridamole	1671:1705	A considerably higher release rate was also observed from tablets with the weakly basic drugs dipyridamole and propranolol hydrochloride containing pH modifier in case of mechanically bio-relevant dissolution models compared to the USP2 apparatus.
34048929	2	88	theme	dissolution	472:482	arg1	USP2					497:500	USP2	497:500	USP2	497:500	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	2	88	theme	dissolution	472:482	arg1	apparatuses					484:494	static dissolution apparatuses	465:494	static dissolution apparatuses (USP2)	465:501	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	2	88	theme	dissolution	472:482	arg1	simulator					557:565	the Advanced gastric simulator	536:565	the Advanced gastric simulator (AGS)	536:571	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	10	89	theme	pH	1902:1903	arg1	dye					1915:1917	a pH indicator dye	1900:1917	a pH indicator dye	1900:1917	For the assessment of the pHM, the incorporation of a pH indicator dye in the HPMC tablet proved to be more suitable, while the cryostatic method was found to be useful only for a rough pHM estimation.
34048929	4	90	theme	passage	906:912	arg1	simulation					886:895	simulation	886:895	simulation of their passage along different compartments of the GIT	886:952	In our study, the occurrence of a pH gradient in the gel layer of the HPMC tablets was observed during simulation of their passage along different compartments of the GIT.
34048929	2	91	theme	peristaltic	616:626	arg1	action					628:633	peristaltic action	616:633	peristaltic action	616:633	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	10	92	from	incorporation	1883:1895	arg1	tablet					1931:1936	the HPMC tablet	1922:1936	the HPMC tablet	1922:1936	For the assessment of the pHM, the incorporation of a pH indicator dye in the HPMC tablet proved to be more suitable, while the cryostatic method was found to be useful only for a rough pHM estimation.
34048929	9	93	theme	basic	1682:1686	arg1	dipyridamole					1694:1705	the weakly basic drugs dipyridamole	1671:1705	the weakly basic drugs dipyridamole	1671:1705	A considerably higher release rate was also observed from tablets with the weakly basic drugs dipyridamole and propranolol hydrochloride containing pH modifier in case of mechanically bio-relevant dissolution models compared to the USP2 apparatus.
34048929	5	94	theme	exposure	1032:1039	arg1	media					991:995	the media composition and duration	987:1020	the media composition and duration of tablet exposure to the surrounding media	987:1064	The pH gradient was affected by the media composition and duration of tablet exposure to the surrounding media.
34048929	5	94	theme	exposure	1032:1039	arg1	duration					1013:1020	duration	1013:1020	duration	1013:1020	The pH gradient was affected by the media composition and duration of tablet exposure to the surrounding media.
34048929	5	94	theme	exposure	1032:1039	arg1	composition					997:1007	composition	997:1007	composition	997:1007	The pH gradient was affected by the media composition and duration of tablet exposure to the surrounding media.
34048929	4	95	theme	GIT	950:952	arg1	compartments					930:941	different compartments	920:941	different compartments of the GIT	920:952	In our study, the occurrence of a pH gradient in the gel layer of the HPMC tablets was observed during simulation of their passage along different compartments of the GIT.
34048929	8	96	theme	higher	1431:1436	arg1	rate					1450:1453	a significantly higher dissolution rate	1415:1453	a significantly higher dissolution rate due to mechanical stress	1415:1478	Our study demonstrates a significantly higher dissolution rate due to mechanical stress during the bio-relevant simulation of GIT transit of the mechanically sensitive HPMC tablets with poorly soluble drugs.
34048929	0	97	theme	transit	27:33	arg1	Effect					0:5	Effect	0:5	Effect of gastrointestinal transit on micro-environmental pH inside HPMC matrix tablets - in vitro study.	0:104	Effect of gastrointestinal transit on micro-environmental pH inside HPMC matrix tablets - in vitro study.
34048929	2	98	theme	mechanistic	380:390	arg1	approaches					392:401	two mechanistic approaches	376:401	two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA))	376:642	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	0	99	theme	micro-environmental	38:56	arg1	tablets					80:86	micro-environmental pH inside HPMC matrix tablets	38:86	micro-environmental pH inside HPMC matrix tablets	38:86	Effect of gastrointestinal transit on micro-environmental pH inside HPMC matrix tablets - in vitro study.
34048929	4	100	theme	different	920:928	arg1	compartments					930:941	different compartments	920:941	different compartments of the GIT	920:952	In our study, the occurrence of a pH gradient in the gel layer of the HPMC tablets was observed during simulation of their passage along different compartments of the GIT.
34048929	0	101	theme	inside	61:66	arg1	tablets					80:86	micro-environmental pH inside HPMC matrix tablets	38:86	micro-environmental pH inside HPMC matrix tablets	38:86	Effect of gastrointestinal transit on micro-environmental pH inside HPMC matrix tablets - in vitro study.
34048929	2	102	theme	dynamic	507:513	arg1	simulator					557:565	the Advanced gastric simulator	536:565	the Advanced gastric simulator (AGS)	536:571	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	2	102	theme	dynamic	507:513	arg1	approaches					515:524	dynamic approaches	507:524	dynamic approaches including the Advanced gastric simulator (AGS)	507:571	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	2	102	theme	dynamic	507:513	arg1	IMSPA					636:640	IMSPA	636:640	IMSPA	636:640	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	2	103	theme	modifying	302:310	arg1	effect					312:317	pH modifying effect	299:317	pH modifying effect on the micro-environmental pH in HPMC matrix	299:362	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	2	104	dep	apparatuses	484:494	arg1	i.e.					460:463	i.e.	460:463	i.e.	460:463	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
34048929	6	105	theme	stress	1151:1156	arg1	influence					1123:1131	the influence	1119:1131	the influence of the mechanical stress on the drug release kinetics	1119:1185	Both dissolution methods were also used to evaluate the influence of the mechanical stress on the drug release kinetics.
34048929	8	106	with	tablets	1565:1571	arg1	drugs					1593:1597	poorly soluble drugs	1578:1597	poorly soluble drugs	1578:1597	Our study demonstrates a significantly higher dissolution rate due to mechanical stress during the bio-relevant simulation of GIT transit of the mechanically sensitive HPMC tablets with poorly soluble drugs.
34048929	8	107	theme	HPMC	1560:1563	arg1	tablets					1565:1571	the mechanically sensitive HPMC tablets	1533:1571	the mechanically sensitive HPMC tablets with poorly soluble drugs	1533:1597	Our study demonstrates a significantly higher dissolution rate due to mechanical stress during the bio-relevant simulation of GIT transit of the mechanically sensitive HPMC tablets with poorly soluble drugs.
34048929	1	108	theme	pH	220:221	arg1	modifiers					223:231	pH modifiers	220:231	pH modifiers	220:231	A commonly used approach to enhance the dissolution of drugs with pH-dependent solubility is the incorporation of pH modifiers.
34048929	7	109	theme	matrix	1376:1381	arg1	tablets					1383:1389	the matrix tablets	1372:1389	the matrix tablets	1372:1389	Micro-environmental pH (pHM) was evaluated, using two methods: the cryostatic method with a surface pH electrode, and with the incorporation of a pH sensitive dye (methyl orange) into the matrix tablets.
34048929	10	110	theme	cryostatic	1976:1985	arg1	useful					2010:2015	useful	2010:2015	useful	2010:2015	For the assessment of the pHM, the incorporation of a pH indicator dye in the HPMC tablet proved to be more suitable, while the cryostatic method was found to be useful only for a rough pHM estimation.
34048929	10	110	theme	cryostatic	1976:1985	arg1	method					1987:1992	the cryostatic method	1972:1992	the cryostatic method	1972:1992	For the assessment of the pHM, the incorporation of a pH indicator dye in the HPMC tablet proved to be more suitable, while the cryostatic method was found to be useful only for a rough pHM estimation.
34048929	8	111	theme	bio-relevant	1491:1502	arg1	simulation					1504:1513	the bio-relevant simulation	1487:1513	the bio-relevant simulation of GIT transit of the mechanically sensitive HPMC tablets with poorly soluble drugs	1487:1597	Our study demonstrates a significantly higher dissolution rate due to mechanical stress during the bio-relevant simulation of GIT transit of the mechanically sensitive HPMC tablets with poorly soluble drugs.
34048929	2	112	theme	micro-environmental	326:344	arg1	pH					346:347	the micro-environmental pH	322:347	the micro-environmental pH in HPMC matrix	322:362	The aim of this study was to evaluate the duration and extent of pH modifying effect on the micro-environmental pH in HPMC matrix by applying two mechanistic approaches regarding hydrodynamic stress on the tested formulation (i.e. static dissolution apparatuses (USP2) and dynamic approaches including the Advanced gastric simulator (AGS) and the Intestinal model for simulation of peristaltic action (IMSPA)).
32597501	6	0	used	used	1372:1375	arg2	encapsulation					1355:1367	in situ encapsulation	1347:1367	in situ encapsulation	1347:1367	Our results indicate an increased protective effect at gastric pH and targeted release of polyphenols when in situ encapsulation is used to encapsulate the extracts.
32597501	10	1	theme	encapsulation	2139:2151	arg1	methods					2153:2159	efficient encapsulation methods	2129:2159	efficient encapsulation methods	2129:2159	In this sense, capsule design and the development of efficient encapsulation methods are very important to obtain a suitable carrier and protector system for the capsulated bioactive extracts or molecules.
32597501	2	2	from	molecules	535:543	arg1	interaction					483:493	the interaction	479:493	the interaction of polymers on capsules and the payload molecules	479:543	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	3	3	from	encapsulation	659:671	arg1	exocarp					731:737	papaya exocarp	724:737	papaya exocarp	724:737	The present study describes the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite, evaluating the performance of gallic acid encapsulation obtained through in situ and two-step entrapment methods.
32597501	3	3	from	encapsulation	659:671	arg1	composite					760:768	a pectin-alginate composite	742:768	a pectin-alginate composite	742:768	The present study describes the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite, evaluating the performance of gallic acid encapsulation obtained through in situ and two-step entrapment methods.
32597501	8	4	from	recovery	1591:1598	arg1	form					1697:1700	the form	1693:1700	the form of by-product extracts	1693:1723	The recovery of bioactive molecules such as polyphenols, for food supplements or formulation additives in the form of by-product extracts is gaining importance as novel sustainable processes in the agricultural industry.
32597501	1	5	theme	compounds	223:231	arg1	variety					202:208	a wide variety	195:208	a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries	195:300	The papaya fruit (Carica papaya L.) contains a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries.
32597501	1	5	theme	compounds	223:231	arg1	compounds					223:231	bioactive compounds	213:231	bioactive compounds with potential applications in the food and nutraceutical industries	213:300	The papaya fruit (Carica papaya L.) contains a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries.
32597501	4	6	theme	best	889:892	arg1	ratio					910:914	The best alginate:pectin ratio	885:914	The best alginate:pectin ratio for gallic acid encapsulation	885:944	The best alginate:pectin ratio for gallic acid encapsulation was 55:45 and 61:39, achieving 6.1 mg and 28.1 mg GAE/g capsules when the papaya exocarp extract was encapsulated by in situ and two-step, respectively.
32597501	4	6	theme	best	889:892	arg1	55:45					950:954	55:45	950:954	55:45	950:954	The best alginate:pectin ratio for gallic acid encapsulation was 55:45 and 61:39, achieving 6.1 mg and 28.1 mg GAE/g capsules when the papaya exocarp extract was encapsulated by in situ and two-step, respectively.
32597501	1	7	theme	potential	238:246	arg1	applications					248:259	potential applications	238:259	potential applications in the food and nutraceutical industries	238:300	The papaya fruit (Carica papaya L.) contains a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries.
32597501	2	8	theme	operational	603:613	arg1	conditions					615:624	operational conditions	603:624	operational conditions	603:624	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	9	9	theme	storage	1943:1949	arg1	stability					1951:1959	the low storage stability	1935:1959	the low storage stability	1935:1959	Thus, the encapsulation of such bioactive extracts for storage and consumption is an active research field, aiming to overcome the low storage stability and lability to gastric conditions, currently hindering their applications in food or pharmaceutical formulations.
32597501	3	10	theme	acid-rich	700:708	arg1	extracts					710:717	trans-ferulic acid-rich extracts	686:717	trans-ferulic acid-rich extracts from papaya exocarp	686:737	The present study describes the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite, evaluating the performance of gallic acid encapsulation obtained through in situ and two-step entrapment methods.
32597501	6	11	theme	protective	1274:1283	arg1	effect					1285:1290	an increased protective effect	1261:1290	an increased protective effect at gastric pH	1261:1304	Our results indicate an increased protective effect at gastric pH and targeted release of polyphenols when in situ encapsulation is used to encapsulate the extracts.
32597501	9	12	theme	extracts	1850:1857	arg1	field					1909:1913	an active research field	1890:1913	an active research field	1890:1913	Thus, the encapsulation of such bioactive extracts for storage and consumption is an active research field, aiming to overcome the low storage stability and lability to gastric conditions, currently hindering their applications in food or pharmaceutical formulations.
32597501	9	12	theme	extracts	1850:1857	arg1	encapsulation					1818:1830	the encapsulation	1814:1830	the encapsulation of such bioactive extracts for storage and consumption	1814:1885	Thus, the encapsulation of such bioactive extracts for storage and consumption is an active research field, aiming to overcome the low storage stability and lability to gastric conditions, currently hindering their applications in food or pharmaceutical formulations.
32597501	8	13	theme	sustainable	1756:1766	arg1	importance					1736:1745	importance	1736:1745	importance	1736:1745	The recovery of bioactive molecules such as polyphenols, for food supplements or formulation additives in the form of by-product extracts is gaining importance as novel sustainable processes in the agricultural industry.
32597501	8	13	theme	sustainable	1756:1766	arg1	processes					1768:1776	novel sustainable processes	1750:1776	novel sustainable processes in the agricultural industry	1750:1805	The recovery of bioactive molecules such as polyphenols, for food supplements or formulation additives in the form of by-product extracts is gaining importance as novel sustainable processes in the agricultural industry.
32597501	2	14	theme	system	590:595	arg1	performance					563:573	the performance	559:573	the performance of the capsule system	559:595	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	12	15	theme	works	2562:2566	arg1	kind					2554:2557	this kind	2549:2557	this kind of works	2549:2566	In conclusion, this kind of works could allow to the application of the agroindustry byproducts to obtain high added-value products, in the form of polyphenol-loaded capsules.
32597501	2	16	from	interaction	483:493	arg1	molecules					535:543	the payload molecules	523:543	the payload molecules	523:543	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	2	16	from	interaction	483:493	arg1	capsules					510:517	capsules	510:517	capsules	510:517	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	1	17	theme	Carica	168:173	arg1	L.					182:183	Carica papaya L.	168:183	Carica papaya L.	168:183	The papaya fruit (Carica papaya L.) contains a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries.
32597501	1	17	theme	Carica	168:173	arg1	fruit					161:165	The papaya fruit	150:165	The papaya fruit (Carica papaya L.)	150:184	The papaya fruit (Carica papaya L.) contains a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries.
32597501	4	18	theme	gallic	920:925	arg1	acid					927:930	gallic acid	920:930	gallic acid encapsulation	920:944	The best alginate:pectin ratio for gallic acid encapsulation was 55:45 and 61:39, achieving 6.1 mg and 28.1 mg GAE/g capsules when the papaya exocarp extract was encapsulated by in situ and two-step, respectively.
32597501	10	19	theme	suitable	2192:2199	arg1	carrier					2201:2207	a suitable carrier and protector system	2190:2228	carrier	2201:2207	In this sense, capsule design and the development of efficient encapsulation methods are very important to obtain a suitable carrier and protector system for the capsulated bioactive extracts or molecules.
32597501	8	20	theme	agricultural	1785:1796	arg1	industry					1798:1805	the agricultural industry	1781:1805	the agricultural industry	1781:1805	The recovery of bioactive molecules such as polyphenols, for food supplements or formulation additives in the form of by-product extracts is gaining importance as novel sustainable processes in the agricultural industry.
32597501	6	21	dep	in	1347:1348	arg1	situ					1350:1353	situ	1350:1353	situ	1350:1353	Our results indicate an increased protective effect at gastric pH and targeted release of polyphenols when in situ encapsulation is used to encapsulate the extracts.
32597501	0	22	theme	papaya	138:143	arg1	L					145:145	Carica papaya L.	131:146	Carica papaya L.	131:146	Development of alginate-pectin microcapsules by the extrusion for encapsulation and controlled release of polyphenols from papaya (Carica papaya L.).
32597501	0	22	theme	papaya	138:143	arg1	papaya					123:128	papaya	123:128	papaya (Carica papaya L.)	123:147	Development of alginate-pectin microcapsules by the extrusion for encapsulation and controlled release of polyphenols from papaya (Carica papaya L.).
32597501	3	23	theme	two-step	856:863	arg1	methods					876:882	in situ and two-step entrapment methods	844:882	in situ and two-step entrapment methods	844:882	The present study describes the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite, evaluating the performance of gallic acid encapsulation obtained through in situ and two-step entrapment methods.
32597501	11	24	theme	targeted	2471:2478	arg1	release					2480:2486	targeted release	2471:2486	targeted release	2471:2486	This research aims to add value to papaya waste and potentially to other agroindustry wastes such as pectin and alginate, resulting in a polyphenol carrier with excellent encapsulation and targeted release properties under gastrointestinal conditions.
32597501	3	25	theme	gallic	801:806	arg1	encapsulation					813:825	gallic acid encapsulation	801:825	gallic acid encapsulation obtained through in situ and two-step entrapment methods	801:882	The present study describes the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite, evaluating the performance of gallic acid encapsulation obtained through in situ and two-step entrapment methods.
32597501	6	26	theme	targeted	1310:1317	arg1	release					1319:1325	targeted release	1310:1325	targeted release of polyphenols when in situ encapsulation is used to encapsulate the extracts	1310:1403	Our results indicate an increased protective effect at gastric pH and targeted release of polyphenols when in situ encapsulation is used to encapsulate the extracts.
32597501	9	27	theme	bioactive	1840:1848	arg1	extracts					1850:1857	such bioactive extracts	1835:1857	such bioactive extracts	1835:1857	Thus, the encapsulation of such bioactive extracts for storage and consumption is an active research field, aiming to overcome the low storage stability and lability to gastric conditions, currently hindering their applications in food or pharmaceutical formulations.
32597501	10	28	theme	bioactive	2249:2257	arg1	extracts					2259:2266	the capsulated bioactive extracts	2234:2266	the capsulated bioactive extracts	2234:2266	In this sense, capsule design and the development of efficient encapsulation methods are very important to obtain a suitable carrier and protector system for the capsulated bioactive extracts or molecules.
32597501	5	29	theme	gastrointestinal	1211:1226	arg1	conditions					1228:1237	gastrointestinal conditions	1211:1237	gastrointestinal conditions	1211:1237	We also evaluated the payload release performance of the obtained capsules under in vitro conditions simulating gastrointestinal conditions.
32597501	2	30	theme	functional	404:413	arg1	storage					443:449	functional, stable, and cost-effective storage and delivery systems	404:470	storage	443:449	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	2	31	theme	systems	464:470	arg1	development					389:399	the development	385:399	the development of functional, stable, and cost-effective storage and delivery systems	385:470	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	2	32	theme	payload	527:533	arg1	molecules					535:543	the payload molecules	523:543	the payload molecules	523:543	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	10	33	theme	capsule	2091:2097	arg1	design					2099:2104	capsule design	2091:2104	capsule design	2091:2104	In this sense, capsule design and the development of efficient encapsulation methods are very important to obtain a suitable carrier and protector system for the capsulated bioactive extracts or molecules.
32597501	0	34	theme	controlled	84:93	arg1	release					95:101	controlled release	84:101	controlled release of polyphenols from papaya (Carica papaya L.)	84:147	Development of alginate-pectin microcapsules by the extrusion for encapsulation and controlled release of polyphenols from papaya (Carica papaya L.).
32597501	5	35	theme	payload	1121:1127	arg1	performance					1137:1147	the payload release performance	1117:1147	the payload release performance of the obtained capsules	1117:1172	We also evaluated the payload release performance of the obtained capsules under in vitro conditions simulating gastrointestinal conditions.
32597501	4	36	dep	in	1063:1064	arg1	situ					1066:1069	situ	1066:1069	situ	1066:1069	The best alginate:pectin ratio for gallic acid encapsulation was 55:45 and 61:39, achieving 6.1 mg and 28.1 mg GAE/g capsules when the papaya exocarp extract was encapsulated by in situ and two-step, respectively.
32597501	2	37	theme	polymers	498:505	arg1	interaction					483:493	the interaction	479:493	the interaction of polymers on capsules and the payload molecules	479:543	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	11	38	theme	polyphenol	2419:2428	arg1	carrier					2430:2436	a polyphenol carrier	2417:2436	a polyphenol carrier with excellent encapsulation and targeted release properties under gastrointestinal conditions	2417:2531	This research aims to add value to papaya waste and potentially to other agroindustry wastes such as pectin and alginate, resulting in a polyphenol carrier with excellent encapsulation and targeted release properties under gastrointestinal conditions.
32597501	3	39	theme	encapsulation	813:825	arg1	performance					786:796	the performance	782:796	the performance of gallic acid encapsulation obtained through in situ and two-step entrapment methods	782:882	The present study describes the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite, evaluating the performance of gallic acid encapsulation obtained through in situ and two-step entrapment methods.
32597501	11	40	theme	papaya	2317:2322	arg1	waste					2324:2328	papaya waste	2317:2328	papaya waste	2317:2328	This research aims to add value to papaya waste and potentially to other agroindustry wastes such as pectin and alginate, resulting in a polyphenol carrier with excellent encapsulation and targeted release properties under gastrointestinal conditions.
32597501	3	41	from	exocarp	731:737	arg1	extracts					710:717	trans-ferulic acid-rich extracts	686:717	trans-ferulic acid-rich extracts from papaya exocarp	686:737	The present study describes the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite, evaluating the performance of gallic acid encapsulation obtained through in situ and two-step entrapment methods.
32597501	3	41	from	exocarp	731:737	arg1	rutin					676:680	rutin	676:680	rutin	676:680	The present study describes the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite, evaluating the performance of gallic acid encapsulation obtained through in situ and two-step entrapment methods.
32597501	3	41	from	exocarp	731:737	arg1	encapsulation					659:671	the encapsulation	655:671	the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite	655:768	The present study describes the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite, evaluating the performance of gallic acid encapsulation obtained through in situ and two-step entrapment methods.
32597501	4	42	theme	papaya	1020:1025	arg1	extract					1035:1041	the papaya exocarp extract	1016:1041	the papaya exocarp extract	1016:1041	The best alginate:pectin ratio for gallic acid encapsulation was 55:45 and 61:39, achieving 6.1 mg and 28.1 mg GAE/g capsules when the papaya exocarp extract was encapsulated by in situ and two-step, respectively.
32597501	11	43	theme	encapsulation	2453:2465	arg1	properties					2488:2497	excellent encapsulation and targeted release properties	2443:2497	excellent encapsulation and targeted release properties under gastrointestinal conditions	2443:2531	This research aims to add value to papaya waste and potentially to other agroindustry wastes such as pectin and alginate, resulting in a polyphenol carrier with excellent encapsulation and targeted release properties under gastrointestinal conditions.
32597501	2	44	dep	entrapment	307:316	arg1	The					303:305	The	303:305	The	303:305	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	5	45	theme	capsules	1165:1172	arg1	performance					1137:1147	the payload release performance	1117:1147	the payload release performance of the obtained capsules	1117:1172	We also evaluated the payload release performance of the obtained capsules under in vitro conditions simulating gastrointestinal conditions.
32597501	12	46	theme	high	2640:2643	arg1	products					2657:2664	high added-value products	2640:2664	high added-value products	2640:2664	In conclusion, this kind of works could allow to the application of the agroindustry byproducts to obtain high added-value products, in the form of polyphenol-loaded capsules.
32597501	0	47	theme	alginate-pectin	15:29	arg1	Development					0:10	Development	0:10	Development of alginate-pectin	0:29	Development of alginate-pectin microcapsules by the extrusion for encapsulation and controlled release of polyphenols from papaya (Carica papaya L.).
32597501	5	48	theme	in	1180:1181	arg1	conditions					1189:1198	in vitro conditions	1180:1198	in vitro conditions simulating gastrointestinal conditions	1180:1237	We also evaluated the payload release performance of the obtained capsules under in vitro conditions simulating gastrointestinal conditions.
32597501	7	49	theme	food	1572:1575	arg1	industry					1577:1584	a more economically and environmentally sustainable food industry	1520:1584	a more economically and environmentally sustainable food industry	1520:1584	PRACTICAL APPLICATIONS: Currently, adding value to agroindustry processing waste is an important focus to achieve a more economically and environmentally sustainable food industry.
32597501	4	50	theme	GAE/g	996:1000	arg1	capsules					1002:1009	6.1 mg and 28.1 mg GAE/g capsules	977:1009	capsules	1002:1009	The best alginate:pectin ratio for gallic acid encapsulation was 55:45 and 61:39, achieving 6.1 mg and 28.1 mg GAE/g capsules when the papaya exocarp extract was encapsulated by in situ and two-step, respectively.
32597501	3	51	theme	present	631:637	arg1	study					639:643	The present study	627:643	The present study	627:643	The present study describes the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite, evaluating the performance of gallic acid encapsulation obtained through in situ and two-step entrapment methods.
32597501	2	52	theme	storage	443:449	arg1	development					389:399	the development	385:399	the development of functional, stable, and cost-effective storage and delivery systems	385:470	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	2	53	theme	cost-effective	428:441	arg1	storage					443:449	functional, stable, and cost-effective storage and delivery systems	404:470	storage	443:449	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	8	54	theme	bioactive	1603:1611	arg1	molecules					1613:1621	bioactive molecules	1603:1621	bioactive molecules such as polyphenols, for food supplements or formulation additives	1603:1688	The recovery of bioactive molecules such as polyphenols, for food supplements or formulation additives in the form of by-product extracts is gaining importance as novel sustainable processes in the agricultural industry.
32597501	8	54	theme	bioactive	1603:1611	arg1	polyphenols					1631:1641	polyphenols	1631:1641	polyphenols	1631:1641	The recovery of bioactive molecules such as polyphenols, for food supplements or formulation additives in the form of by-product extracts is gaining importance as novel sustainable processes in the agricultural industry.
32597501	7	55	theme	economically	1527:1538	arg1	industry					1577:1584	a more economically and environmentally sustainable food industry	1520:1584	a more economically and environmentally sustainable food industry	1520:1584	PRACTICAL APPLICATIONS: Currently, adding value to agroindustry processing waste is an important focus to achieve a more economically and environmentally sustainable food industry.
32597501	9	56	theme	active	1893:1898	arg1	field					1909:1913	an active research field	1890:1913	an active research field	1890:1913	Thus, the encapsulation of such bioactive extracts for storage and consumption is an active research field, aiming to overcome the low storage stability and lability to gastric conditions, currently hindering their applications in food or pharmaceutical formulations.
32597501	9	56	theme	active	1893:1898	arg1	encapsulation					1818:1830	the encapsulation	1814:1830	the encapsulation of such bioactive extracts for storage and consumption	1814:1885	Thus, the encapsulation of such bioactive extracts for storage and consumption is an active research field, aiming to overcome the low storage stability and lability to gastric conditions, currently hindering their applications in food or pharmaceutical formulations.
32597501	10	57	theme	capsulated	2238:2247	arg1	extracts					2259:2266	the capsulated bioactive extracts	2234:2266	the capsulated bioactive extracts	2234:2266	In this sense, capsule design and the development of efficient encapsulation methods are very important to obtain a suitable carrier and protector system for the capsulated bioactive extracts or molecules.
32597501	11	58	theme	agroindustry	2355:2366	arg1	alginate					2394:2401	alginate	2394:2401	alginate	2394:2401	This research aims to add value to papaya waste and potentially to other agroindustry wastes such as pectin and alginate, resulting in a polyphenol carrier with excellent encapsulation and targeted release properties under gastrointestinal conditions.
32597501	11	58	theme	agroindustry	2355:2366	arg1	wastes					2368:2373	other agroindustry wastes	2349:2373	other agroindustry wastes such as pectin and alginate, resulting in a polyphenol carrier with excellent encapsulation and targeted release properties under gastrointestinal conditions	2349:2531	This research aims to add value to papaya waste and potentially to other agroindustry wastes such as pectin and alginate, resulting in a polyphenol carrier with excellent encapsulation and targeted release properties under gastrointestinal conditions.
32597501	11	58	theme	agroindustry	2355:2366	arg1	pectin					2383:2388	pectin	2383:2388	pectin	2383:2388	This research aims to add value to papaya waste and potentially to other agroindustry wastes such as pectin and alginate, resulting in a polyphenol carrier with excellent encapsulation and targeted release properties under gastrointestinal conditions.
32597501	2	59	theme	compounds	348:356	arg1	release					322:328	release	322:328	release	322:328	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	2	59	theme	compounds	348:356	arg1	entrapment					307:316	entrapment	307:316	entrapment	307:316	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	10	60	theme	methods	2153:2159	arg1	important					2170:2178	important	2170:2178	important	2170:2178	In this sense, capsule design and the development of efficient encapsulation methods are very important to obtain a suitable carrier and protector system for the capsulated bioactive extracts or molecules.
32597501	10	60	theme	methods	2153:2159	arg1	design					2099:2104	capsule design	2091:2104	capsule design	2091:2104	In this sense, capsule design and the development of efficient encapsulation methods are very important to obtain a suitable carrier and protector system for the capsulated bioactive extracts or molecules.
32597501	10	60	theme	methods	2153:2159	arg1	development					2114:2124	the development	2110:2124	the development of efficient encapsulation methods	2110:2159	In this sense, capsule design and the development of efficient encapsulation methods are very important to obtain a suitable carrier and protector system for the capsulated bioactive extracts or molecules.
32597501	3	61	theme	trans-ferulic	686:698	arg1	extracts					710:717	trans-ferulic acid-rich extracts	686:717	trans-ferulic acid-rich extracts from papaya exocarp	686:737	The present study describes the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite, evaluating the performance of gallic acid encapsulation obtained through in situ and two-step entrapment methods.
32597501	2	62	theme	such	333:336	arg1	compounds					348:356	such bioactive compounds	333:356	such bioactive compounds	333:356	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	8	63	theme	by-product	1705:1714	arg1	extracts					1716:1723	by-product extracts	1705:1723	by-product extracts	1705:1723	The recovery of bioactive molecules such as polyphenols, for food supplements or formulation additives in the form of by-product extracts is gaining importance as novel sustainable processes in the agricultural industry.
32597501	1	64	theme	bioactive	213:221	arg1	compounds					223:231	bioactive compounds	213:231	bioactive compounds with potential applications in the food and nutraceutical industries	213:300	The papaya fruit (Carica papaya L.) contains a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries.
32597501	7	65	theme	important	1493:1501	arg1	focus					1503:1507	an important focus	1490:1507	an important focus to achieve a more economically and environmentally sustainable food industry	1490:1584	PRACTICAL APPLICATIONS: Currently, adding value to agroindustry processing waste is an important focus to achieve a more economically and environmentally sustainable food industry.
32597501	1	66	from	applications	248:259	arg1	industries					291:300	the food and nutraceutical industries	264:300	the food and nutraceutical industries	264:300	The papaya fruit (Carica papaya L.) contains a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries.
32597501	10	67	theme	efficient	2129:2137	arg1	methods					2153:2159	efficient encapsulation methods	2129:2159	efficient encapsulation methods	2129:2159	In this sense, capsule design and the development of efficient encapsulation methods are very important to obtain a suitable carrier and protector system for the capsulated bioactive extracts or molecules.
32597501	3	68	theme	rutin	676:680	arg1	encapsulation					659:671	the encapsulation	655:671	the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite	655:768	The present study describes the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite, evaluating the performance of gallic acid encapsulation obtained through in situ and two-step entrapment methods.
32597501	6	69	theme	gastric	1295:1301	arg1	pH					1303:1304	gastric pH	1295:1304	gastric pH	1295:1304	Our results indicate an increased protective effect at gastric pH and targeted release of polyphenols when in situ encapsulation is used to encapsulate the extracts.
32597501	11	70	theme	gastrointestinal	2505:2520	arg1	conditions					2522:2531	gastrointestinal conditions	2505:2531	gastrointestinal conditions	2505:2531	This research aims to add value to papaya waste and potentially to other agroindustry wastes such as pectin and alginate, resulting in a polyphenol carrier with excellent encapsulation and targeted release properties under gastrointestinal conditions.
32597501	4	71	theme	6.1 mg	977:982	arg1	capsules					1002:1009	6.1 mg and 28.1 mg GAE/g capsules	977:1009	capsules	1002:1009	The best alginate:pectin ratio for gallic acid encapsulation was 55:45 and 61:39, achieving 6.1 mg and 28.1 mg GAE/g capsules when the papaya exocarp extract was encapsulated by in situ and two-step, respectively.
32597501	7	72	theme	agroindustry	1457:1468	arg1	waste					1481:1485	agroindustry processing waste	1457:1485	agroindustry processing waste	1457:1485	PRACTICAL APPLICATIONS: Currently, adding value to agroindustry processing waste is an important focus to achieve a more economically and environmentally sustainable food industry.
32597501	9	73	theme	low	1939:1941	arg1	stability					1951:1959	the low storage stability	1935:1959	the low storage stability	1935:1959	Thus, the encapsulation of such bioactive extracts for storage and consumption is an active research field, aiming to overcome the low storage stability and lability to gastric conditions, currently hindering their applications in food or pharmaceutical formulations.
32597501	8	74	theme	formulation	1668:1678	arg1	additives					1680:1688	formulation additives	1668:1688	formulation additives	1668:1688	The recovery of bioactive molecules such as polyphenols, for food supplements or formulation additives in the form of by-product extracts is gaining importance as novel sustainable processes in the agricultural industry.
32597501	3	75	theme	papaya	724:729	arg1	exocarp					731:737	papaya exocarp	724:737	papaya exocarp	724:737	The present study describes the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite, evaluating the performance of gallic acid encapsulation obtained through in situ and two-step entrapment methods.
32597501	1	76	theme	papaya	154:159	arg1	L.					182:183	Carica papaya L.	168:183	Carica papaya L.	168:183	The papaya fruit (Carica papaya L.) contains a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries.
32597501	1	76	theme	papaya	154:159	arg1	fruit					161:165	The papaya fruit	150:165	The papaya fruit (Carica papaya L.)	150:184	The papaya fruit (Carica papaya L.) contains a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries.
32597501	7	77	theme	processing	1470:1479	arg1	waste					1481:1485	agroindustry processing waste	1457:1485	agroindustry processing waste	1457:1485	PRACTICAL APPLICATIONS: Currently, adding value to agroindustry processing waste is an important focus to achieve a more economically and environmentally sustainable food industry.
32597501	3	78	theme	extracts	710:717	arg1	encapsulation					659:671	the encapsulation	655:671	the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite	655:768	The present study describes the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite, evaluating the performance of gallic acid encapsulation obtained through in situ and two-step entrapment methods.
32597501	6	79	theme	increased	1264:1272	arg1	effect					1285:1290	an increased protective effect	1261:1290	an increased protective effect at gastric pH	1261:1304	Our results indicate an increased protective effect at gastric pH and targeted release of polyphenols when in situ encapsulation is used to encapsulate the extracts.
32597501	8	80	theme	novel	1750:1754	arg1	importance					1736:1745	importance	1736:1745	importance	1736:1745	The recovery of bioactive molecules such as polyphenols, for food supplements or formulation additives in the form of by-product extracts is gaining importance as novel sustainable processes in the agricultural industry.
32597501	8	80	theme	novel	1750:1754	arg1	processes					1768:1776	novel sustainable processes	1750:1776	novel sustainable processes in the agricultural industry	1750:1805	The recovery of bioactive molecules such as polyphenols, for food supplements or formulation additives in the form of by-product extracts is gaining importance as novel sustainable processes in the agricultural industry.
32597501	11	81	with	carrier	2430:2436	arg1	properties					2488:2497	excellent encapsulation and targeted release properties	2443:2497	excellent encapsulation and targeted release properties under gastrointestinal conditions	2443:2531	This research aims to add value to papaya waste and potentially to other agroindustry wastes such as pectin and alginate, resulting in a polyphenol carrier with excellent encapsulation and targeted release properties under gastrointestinal conditions.
32597501	2	82	theme	capsule	582:588	arg1	system					590:595	the capsule system	578:595	the capsule system	578:595	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	3	83	theme	pectin-alginate	744:758	arg1	composite					760:768	a pectin-alginate composite	742:768	a pectin-alginate composite	742:768	The present study describes the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite, evaluating the performance of gallic acid encapsulation obtained through in situ and two-step entrapment methods.
32597501	0	84	theme	Carica	131:136	arg1	L					145:145	Carica papaya L.	131:146	Carica papaya L.	131:146	Development of alginate-pectin microcapsules by the extrusion for encapsulation and controlled release of polyphenols from papaya (Carica papaya L.).
32597501	0	84	theme	Carica	131:136	arg1	papaya					123:128	papaya	123:128	papaya (Carica papaya L.)	123:147	Development of alginate-pectin microcapsules by the extrusion for encapsulation and controlled release of polyphenols from papaya (Carica papaya L.).
32597501	1	85	theme	papaya	175:180	arg1	L.					182:183	Carica papaya L.	168:183	Carica papaya L.	168:183	The papaya fruit (Carica papaya L.) contains a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries.
32597501	1	85	theme	papaya	175:180	arg1	fruit					161:165	The papaya fruit	150:165	The papaya fruit (Carica papaya L.)	150:184	The papaya fruit (Carica papaya L.) contains a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries.
32597501	9	86	theme	gastric	1977:1983	arg1	conditions					1985:1994	gastric conditions	1977:1994	gastric conditions	1977:1994	Thus, the encapsulation of such bioactive extracts for storage and consumption is an active research field, aiming to overcome the low storage stability and lability to gastric conditions, currently hindering their applications in food or pharmaceutical formulations.
32597501	6	87	theme	in	1347:1348	arg1	encapsulation					1355:1367	in situ encapsulation	1347:1367	in situ encapsulation	1347:1367	Our results indicate an increased protective effect at gastric pH and targeted release of polyphenols when in situ encapsulation is used to encapsulate the extracts.
32597501	4	88	theme	acid	927:930	arg1	encapsulation					932:944	gallic acid encapsulation	920:944	gallic acid encapsulation	920:944	The best alginate:pectin ratio for gallic acid encapsulation was 55:45 and 61:39, achieving 6.1 mg and 28.1 mg GAE/g capsules when the papaya exocarp extract was encapsulated by in situ and two-step, respectively.
32597501	7	89	theme	PRACTICAL	1406:1414	arg1	APPLICATIONS					1416:1427	PRACTICAL APPLICATIONS	1406:1427	PRACTICAL APPLICATIONS: Currently, adding value to agroindustry processing waste is an important focus to achieve a more economically and environmentally sustainable food industry.	1406:1585	PRACTICAL APPLICATIONS: Currently, adding value to agroindustry processing waste is an important focus to achieve a more economically and environmentally sustainable food industry.
32597501	11	90	theme	release	2480:2486	arg1	properties					2488:2497	excellent encapsulation and targeted release properties	2443:2497	excellent encapsulation and targeted release properties under gastrointestinal conditions	2443:2531	This research aims to add value to papaya waste and potentially to other agroindustry wastes such as pectin and alginate, resulting in a polyphenol carrier with excellent encapsulation and targeted release properties under gastrointestinal conditions.
32597501	6	91	theme	polyphenols	1330:1340	arg1	effect					1285:1290	an increased protective effect	1261:1290	an increased protective effect at gastric pH	1261:1304	Our results indicate an increased protective effect at gastric pH and targeted release of polyphenols when in situ encapsulation is used to encapsulate the extracts.
32597501	6	91	theme	polyphenols	1330:1340	arg1	release					1319:1325	targeted release	1310:1325	targeted release of polyphenols when in situ encapsulation is used to encapsulate the extracts	1310:1403	Our results indicate an increased protective effect at gastric pH and targeted release of polyphenols when in situ encapsulation is used to encapsulate the extracts.
32597501	4	92	theme	alginate	894:901	arg1	ratio					910:914	The best alginate:pectin ratio	885:914	The best alginate:pectin ratio for gallic acid encapsulation	885:944	The best alginate:pectin ratio for gallic acid encapsulation was 55:45 and 61:39, achieving 6.1 mg and 28.1 mg GAE/g capsules when the papaya exocarp extract was encapsulated by in situ and two-step, respectively.
32597501	4	92	theme	alginate	894:901	arg1	55:45					950:954	55:45	950:954	55:45	950:954	The best alginate:pectin ratio for gallic acid encapsulation was 55:45 and 61:39, achieving 6.1 mg and 28.1 mg GAE/g capsules when the papaya exocarp extract was encapsulated by in situ and two-step, respectively.
32597501	0	93	from	papaya	123:128	arg1	encapsulation					66:78	encapsulation	66:78	encapsulation	66:78	Development of alginate-pectin microcapsules by the extrusion for encapsulation and controlled release of polyphenols from papaya (Carica papaya L.).
32597501	0	93	from	papaya	123:128	arg1	release					95:101	controlled release	84:101	controlled release of polyphenols from papaya (Carica papaya L.)	84:147	Development of alginate-pectin microcapsules by the extrusion for encapsulation and controlled release of polyphenols from papaya (Carica papaya L.).
32597501	10	94	theme	protector	2213:2221	arg1	system					2223:2228	a suitable carrier and protector system	2190:2228	system	2223:2228	In this sense, capsule design and the development of efficient encapsulation methods are very important to obtain a suitable carrier and protector system for the capsulated bioactive extracts or molecules.
32597501	3	95	theme	entrapment	865:874	arg1	methods					876:882	in situ and two-step entrapment methods	844:882	in situ and two-step entrapment methods	844:882	The present study describes the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite, evaluating the performance of gallic acid encapsulation obtained through in situ and two-step entrapment methods.
32597501	4	96	theme	pectin	903:908	arg1	ratio					910:914	The best alginate:pectin ratio	885:914	The best alginate:pectin ratio for gallic acid encapsulation	885:944	The best alginate:pectin ratio for gallic acid encapsulation was 55:45 and 61:39, achieving 6.1 mg and 28.1 mg GAE/g capsules when the papaya exocarp extract was encapsulated by in situ and two-step, respectively.
32597501	4	96	theme	pectin	903:908	arg1	55:45					950:954	55:45	950:954	55:45	950:954	The best alginate:pectin ratio for gallic acid encapsulation was 55:45 and 61:39, achieving 6.1 mg and 28.1 mg GAE/g capsules when the papaya exocarp extract was encapsulated by in situ and two-step, respectively.
32597501	2	97	from	polymers	498:505	arg1	molecules					535:543	the payload molecules	523:543	the payload molecules	523:543	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	2	97	from	polymers	498:505	arg1	capsules					510:517	capsules	510:517	capsules	510:517	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	11	98	theme	other	2349:2353	arg1	alginate					2394:2401	alginate	2394:2401	alginate	2394:2401	This research aims to add value to papaya waste and potentially to other agroindustry wastes such as pectin and alginate, resulting in a polyphenol carrier with excellent encapsulation and targeted release properties under gastrointestinal conditions.
32597501	11	98	theme	other	2349:2353	arg1	wastes					2368:2373	other agroindustry wastes	2349:2373	other agroindustry wastes such as pectin and alginate, resulting in a polyphenol carrier with excellent encapsulation and targeted release properties under gastrointestinal conditions	2349:2531	This research aims to add value to papaya waste and potentially to other agroindustry wastes such as pectin and alginate, resulting in a polyphenol carrier with excellent encapsulation and targeted release properties under gastrointestinal conditions.
32597501	11	98	theme	other	2349:2353	arg1	pectin					2383:2388	pectin	2383:2388	pectin	2383:2388	This research aims to add value to papaya waste and potentially to other agroindustry wastes such as pectin and alginate, resulting in a polyphenol carrier with excellent encapsulation and targeted release properties under gastrointestinal conditions.
32597501	3	99	theme	acid	808:811	arg1	encapsulation					813:825	gallic acid encapsulation	801:825	gallic acid encapsulation obtained through in situ and two-step entrapment methods	801:882	The present study describes the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite, evaluating the performance of gallic acid encapsulation obtained through in situ and two-step entrapment methods.
32597501	3	100	dep	in	844:845	arg1	situ					847:850	situ	847:850	situ	847:850	The present study describes the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite, evaluating the performance of gallic acid encapsulation obtained through in situ and two-step entrapment methods.
32597501	7	101	dep	APPLICATIONS	1416:1427	arg1	focus					1503:1507	an important focus	1490:1507	an important focus to achieve a more economically and environmentally sustainable food industry	1490:1584	PRACTICAL APPLICATIONS: Currently, adding value to agroindustry processing waste is an important focus to achieve a more economically and environmentally sustainable food industry.
32597501	5	102	dep	in	1180:1181	arg1	vitro					1183:1187	vitro	1183:1187	vitro	1183:1187	We also evaluated the payload release performance of the obtained capsules under in vitro conditions simulating gastrointestinal conditions.
32597501	9	103	theme	food	2039:2042	arg1	formulations					2062:2073	food or pharmaceutical formulations	2039:2073	food or pharmaceutical formulations	2039:2073	Thus, the encapsulation of such bioactive extracts for storage and consumption is an active research field, aiming to overcome the low storage stability and lability to gastric conditions, currently hindering their applications in food or pharmaceutical formulations.
32597501	2	104	theme	delivery	455:462	arg1	systems					464:470	functional, stable, and cost-effective storage and delivery systems	404:470	systems	464:470	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	12	105	theme	capsules	2700:2707	arg1	form					2674:2677	the form	2670:2677	the form of polyphenol-loaded capsules	2670:2707	In conclusion, this kind of works could allow to the application of the agroindustry byproducts to obtain high added-value products, in the form of polyphenol-loaded capsules.
32597501	9	106	theme	pharmaceutical	2047:2060	arg1	formulations					2062:2073	food or pharmaceutical formulations	2039:2073	food or pharmaceutical formulations	2039:2073	Thus, the encapsulation of such bioactive extracts for storage and consumption is an active research field, aiming to overcome the low storage stability and lability to gastric conditions, currently hindering their applications in food or pharmaceutical formulations.
32597501	3	107	theme	in	844:845	arg1	methods					876:882	in situ and two-step entrapment methods	844:882	in situ and two-step entrapment methods	844:882	The present study describes the encapsulation of rutin and trans-ferulic acid-rich extracts from papaya exocarp in a pectin-alginate composite, evaluating the performance of gallic acid encapsulation obtained through in situ and two-step entrapment methods.
32597501	0	108	theme	polyphenols	106:116	arg1	encapsulation					66:78	encapsulation	66:78	encapsulation	66:78	Development of alginate-pectin microcapsules by the extrusion for encapsulation and controlled release of polyphenols from papaya (Carica papaya L.).
32597501	0	108	theme	polyphenols	106:116	arg1	release					95:101	controlled release	84:101	controlled release of polyphenols from papaya (Carica papaya L.)	84:147	Development of alginate-pectin microcapsules by the extrusion for encapsulation and controlled release of polyphenols from papaya (Carica papaya L.).
32597501	5	109	theme	release	1129:1135	arg1	performance					1137:1147	the payload release performance	1117:1147	the payload release performance of the obtained capsules	1117:1172	We also evaluated the payload release performance of the obtained capsules under in vitro conditions simulating gastrointestinal conditions.
32597501	12	110	theme	byproducts	2619:2628	arg1	application					2587:2597	the application	2583:2597	the application of the agroindustry byproducts	2583:2628	In conclusion, this kind of works could allow to the application of the agroindustry byproducts to obtain high added-value products, in the form of polyphenol-loaded capsules.
32597501	9	111	theme	such	1835:1838	arg1	extracts					1850:1857	such bioactive extracts	1835:1857	such bioactive extracts	1835:1857	Thus, the encapsulation of such bioactive extracts for storage and consumption is an active research field, aiming to overcome the low storage stability and lability to gastric conditions, currently hindering their applications in food or pharmaceutical formulations.
32597501	11	112	theme	excellent	2443:2451	arg1	encapsulation					2453:2465	excellent encapsulation	2443:2465	excellent encapsulation	2443:2465	This research aims to add value to papaya waste and potentially to other agroindustry wastes such as pectin and alginate, resulting in a polyphenol carrier with excellent encapsulation and targeted release properties under gastrointestinal conditions.
32597501	2	113	theme	critical	367:374	arg1	step					376:379	a critical step	365:379	a critical step for the development of functional, stable, and cost-effective storage and delivery systems	365:470	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	9	114	from	applications	2023:2034	arg1	formulations					2062:2073	food or pharmaceutical formulations	2039:2073	food or pharmaceutical formulations	2039:2073	Thus, the encapsulation of such bioactive extracts for storage and consumption is an active research field, aiming to overcome the low storage stability and lability to gastric conditions, currently hindering their applications in food or pharmaceutical formulations.
32597501	2	115	theme	stable	416:421	arg1	storage					443:449	functional, stable, and cost-effective storage and delivery systems	404:470	storage	443:449	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	12	116	theme	added-value	2645:2655	arg1	products					2657:2664	high added-value products	2640:2664	high added-value products	2640:2664	In conclusion, this kind of works could allow to the application of the agroindustry byproducts to obtain high added-value products, in the form of polyphenol-loaded capsules.
32597501	4	117	theme	exocarp	1027:1033	arg1	extract					1035:1041	the papaya exocarp extract	1016:1041	the papaya exocarp extract	1016:1041	The best alginate:pectin ratio for gallic acid encapsulation was 55:45 and 61:39, achieving 6.1 mg and 28.1 mg GAE/g capsules when the papaya exocarp extract was encapsulated by in situ and two-step, respectively.
32597501	1	118	theme	food	268:271	arg1	industries					291:300	the food and nutraceutical industries	264:300	the food and nutraceutical industries	264:300	The papaya fruit (Carica papaya L.) contains a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries.
32597501	4	119	theme	28.1 mg	988:994	arg1	capsules					1002:1009	6.1 mg and 28.1 mg GAE/g capsules	977:1009	capsules	1002:1009	The best alginate:pectin ratio for gallic acid encapsulation was 55:45 and 61:39, achieving 6.1 mg and 28.1 mg GAE/g capsules when the papaya exocarp extract was encapsulated by in situ and two-step, respectively.
32597501	8	120	theme	molecules	1613:1621	arg1	recovery					1591:1598	The recovery	1587:1598	The recovery of bioactive molecules such as polyphenols, for food supplements or formulation additives in the form of by-product extracts	1587:1723	The recovery of bioactive molecules such as polyphenols, for food supplements or formulation additives in the form of by-product extracts is gaining importance as novel sustainable processes in the agricultural industry.
32597501	1	121	theme	nutraceutical	277:289	arg1	industries					291:300	the food and nutraceutical industries	264:300	the food and nutraceutical industries	264:300	The papaya fruit (Carica papaya L.) contains a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries.
32597501	10	122	from	sense	2084:2088	arg1	important					2170:2178	important	2170:2178	important	2170:2178	In this sense, capsule design and the development of efficient encapsulation methods are very important to obtain a suitable carrier and protector system for the capsulated bioactive extracts or molecules.
32597501	10	122	from	sense	2084:2088	arg1	development					2114:2124	the development	2110:2124	the development of efficient encapsulation methods	2110:2159	In this sense, capsule design and the development of efficient encapsulation methods are very important to obtain a suitable carrier and protector system for the capsulated bioactive extracts or molecules.
32597501	7	123	theme	sustainable	1560:1570	arg1	industry					1577:1584	a more economically and environmentally sustainable food industry	1520:1584	a more economically and environmentally sustainable food industry	1520:1584	PRACTICAL APPLICATIONS: Currently, adding value to agroindustry processing waste is an important focus to achieve a more economically and environmentally sustainable food industry.
32597501	1	124	contain	contains	186:193	arg1	L.					182:183	Carica papaya L.	168:183	Carica papaya L.	168:183	The papaya fruit (Carica papaya L.) contains a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries.
32597501	1	124	contain	contains	186:193	arg2	compounds					223:231	bioactive compounds	213:231	bioactive compounds with potential applications in the food and nutraceutical industries	213:300	The papaya fruit (Carica papaya L.) contains a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries.
32597501	1	124	contain	contains	186:193	arg1	fruit					161:165	The papaya fruit	150:165	The papaya fruit (Carica papaya L.)	150:184	The papaya fruit (Carica papaya L.) contains a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries.
32597501	1	124	contain	contains	186:193	arg2	variety					202:208	a wide variety	195:208	a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries	195:300	The papaya fruit (Carica papaya L.) contains a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries.
32597501	10	125	from	important	2170:2178	arg1	sense					2084:2088	this sense	2079:2088	this sense	2079:2088	In this sense, capsule design and the development of efficient encapsulation methods are very important to obtain a suitable carrier and protector system for the capsulated bioactive extracts or molecules.
32597501	5	126	theme	obtained	1156:1163	arg1	capsules					1165:1172	the obtained capsules	1152:1172	the obtained capsules	1152:1172	We also evaluated the payload release performance of the obtained capsules under in vitro conditions simulating gastrointestinal conditions.
32597501	1	127	with	compounds	223:231	arg1	applications					248:259	potential applications	238:259	potential applications in the food and nutraceutical industries	238:300	The papaya fruit (Carica papaya L.) contains a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries.
32597501	9	128	theme	research	1900:1907	arg1	field					1909:1913	an active research field	1890:1913	an active research field	1890:1913	Thus, the encapsulation of such bioactive extracts for storage and consumption is an active research field, aiming to overcome the low storage stability and lability to gastric conditions, currently hindering their applications in food or pharmaceutical formulations.
32597501	9	128	theme	research	1900:1907	arg1	encapsulation					1818:1830	the encapsulation	1814:1830	the encapsulation of such bioactive extracts for storage and consumption	1814:1885	Thus, the encapsulation of such bioactive extracts for storage and consumption is an active research field, aiming to overcome the low storage stability and lability to gastric conditions, currently hindering their applications in food or pharmaceutical formulations.
32597501	2	129	from	capsules	510:517	arg1	interaction					483:493	the interaction	479:493	the interaction of polymers on capsules and the payload molecules	479:543	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	12	130	theme	polyphenol-loaded	2682:2698	arg1	capsules					2700:2707	polyphenol-loaded capsules	2682:2707	polyphenol-loaded capsules	2682:2707	In conclusion, this kind of works could allow to the application of the agroindustry byproducts to obtain high added-value products, in the form of polyphenol-loaded capsules.
32597501	1	131	theme	wide	197:200	arg1	variety					202:208	a wide variety	195:208	a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries	195:300	The papaya fruit (Carica papaya L.) contains a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries.
32597501	1	131	theme	wide	197:200	arg1	compounds					223:231	bioactive compounds	213:231	bioactive compounds with potential applications in the food and nutraceutical industries	213:300	The papaya fruit (Carica papaya L.) contains a wide variety of bioactive compounds with potential applications in the food and nutraceutical industries.
32597501	12	132	theme	agroindustry	2606:2617	arg1	byproducts					2619:2628	the agroindustry byproducts	2602:2628	the agroindustry byproducts	2602:2628	In conclusion, this kind of works could allow to the application of the agroindustry byproducts to obtain high added-value products, in the form of polyphenol-loaded capsules.
32597501	8	133	theme	food	1648:1651	arg1	supplements					1653:1663	food supplements	1648:1663	food supplements	1648:1663	The recovery of bioactive molecules such as polyphenols, for food supplements or formulation additives in the form of by-product extracts is gaining importance as novel sustainable processes in the agricultural industry.
32597501	8	134	from	processes	1768:1776	arg1	industry					1798:1805	the agricultural industry	1781:1805	the agricultural industry	1781:1805	The recovery of bioactive molecules such as polyphenols, for food supplements or formulation additives in the form of by-product extracts is gaining importance as novel sustainable processes in the agricultural industry.
32597501	2	135	theme	bioactive	338:346	arg1	compounds					348:356	such bioactive compounds	333:356	such bioactive compounds	333:356	The entrapment and release of such bioactive compounds remain a critical step for the development of functional, stable, and cost-effective storage and delivery systems, since the interaction of polymers on capsules and the payload molecules can influence the performance of the capsule system under operational conditions.
32597501	8	136	theme	extracts	1716:1723	arg1	form					1697:1700	the form	1693:1700	the form of by-product extracts	1693:1723	The recovery of bioactive molecules such as polyphenols, for food supplements or formulation additives in the form of by-product extracts is gaining importance as novel sustainable processes in the agricultural industry.
32597501	6	137	from	pH	1303:1304	arg1	effect					1285:1290	an increased protective effect	1261:1290	an increased protective effect at gastric pH	1261:1304	Our results indicate an increased protective effect at gastric pH and targeted release of polyphenols when in situ encapsulation is used to encapsulate the extracts.
32597501	6	137	from	pH	1303:1304	arg1	release					1319:1325	targeted release	1310:1325	targeted release of polyphenols when in situ encapsulation is used to encapsulate the extracts	1310:1403	Our results indicate an increased protective effect at gastric pH and targeted release of polyphenols when in situ encapsulation is used to encapsulate the extracts.
34973989	6	0	dep	MCF-7	1069:1073	arg1	cancer					1088:1093	human breast cancer	1075:1093	human breast cancer	1075:1093	Additionally, we evaluated the anti-cancer activity of these nanocarriers through WST-1 analysis and acridine orange staining in MCF-7 human breast cancer and HUVEC control cell lines.
34973989	0	1	theme	cancer	199:204	arg1	therapy					206:212	breast cancer therapy	192:212	breast cancer therapy	192:212	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	2	2	used	used	430:433	arg2	approach					417:424	Impregnation approach	404:424	Impregnation approach	404:424	Impregnation approach was used for encapsulation of 5-Fluorouracil alone and with GEM inside the MIL-100.
34973989	4	3	theme	hybrid	739:744	arg1	nascency					758:765	The hybrid nanocarrier nascency	735:765	The hybrid nanocarrier nascency	735:765	The hybrid nanocarrier nascency was verified by different characterization results.
34973989	0	4	theme	breast	192:197	arg1	therapy					206:212	breast cancer therapy	192:212	breast cancer therapy	192:212	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	1	5	theme	therapeutic	272:282	arg1	efficiency					284:293	the bioavailability and therapeutic efficiency	248:293	the bioavailability and therapeutic efficiency of Gemcitabine (GEM) and 5-Fluorouracil with decreased side effects using MIL-100 nano-composite as carrier	248:401	This study was planned to evolve the bioavailability and therapeutic efficiency of Gemcitabine (GEM) and 5-Fluorouracil with decreased side effects using MIL-100 nano-composite as carrier.
34973989	0	6	theme	advanced	120:127	arg1	composite					104:112	chitosan-coated MIL-100 composite	80:112	chitosan-coated MIL-100 composite	80:112	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	0	6	theme	advanced	120:127	arg1	system					182:187	an advanced, biocompatible, pH-sensitive and smart drug delivery system	117:187	an advanced, biocompatible, pH-sensitive and smart drug delivery system	117:187	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	11	7	theme	chemotherapeutic	1660:1675	arg1	drugs					1677:1681	promising chemotherapeutic drugs	1650:1681	a promising chemotherapeutic drugs delivery	1648:1690	Therefore, MIL-100 could be used for a promising chemotherapeutic drugs delivery and chitosan coated drugs with quercetin could be useful for reducing toxicity on normal cells.
34973989	6	8	theme	human	1075:1079	arg1	cancer					1088:1093	human breast cancer	1075:1093	human breast cancer	1075:1093	Additionally, we evaluated the anti-cancer activity of these nanocarriers through WST-1 analysis and acridine orange staining in MCF-7 human breast cancer and HUVEC control cell lines.
34973989	8	9	theme	5-Fluorouracil	1245:1258	arg1	MIL-100					1260:1266	5-Fluorouracil@MIL-100	1245:1266	5-Fluorouracil@MIL-100	1245:1266	However, 5-Fluorouracil@MIL-100 and chitosan-coated 5-Fluorouracil@MIL-100 with quercetin were more effective than other nanocarriers in MCF-7 cells (p < 0.05).
34973989	6	10	theme	anti-cancer	971:981	arg1	activity					983:990	the anti-cancer activity	967:990	the anti-cancer activity of these nanocarriers	967:1012	Additionally, we evaluated the anti-cancer activity of these nanocarriers through WST-1 analysis and acridine orange staining in MCF-7 human breast cancer and HUVEC control cell lines.
34973989	10	11	theme	coated	1520:1525	arg1	nanocarriers					1527:1538	chitosan coated nanocarriers	1511:1538	chitosan coated nanocarriers with quercetin	1511:1553	However, chitosan coated nanocarriers with quercetin were less toxic on HUVEC cells at particularly 1 µg/mL.
34973989	10	12	with	nanocarriers	1527:1538	arg1	quercetin					1545:1553	quercetin	1545:1553	quercetin	1545:1553	However, chitosan coated nanocarriers with quercetin were less toxic on HUVEC cells at particularly 1 µg/mL.
34973989	8	13	theme	MCF-7	1373:1377	arg1	p < 0.05					1386:1393	p < 0.05	1386:1393	p < 0.05	1386:1393	However, 5-Fluorouracil@MIL-100 and chitosan-coated 5-Fluorouracil@MIL-100 with quercetin were more effective than other nanocarriers in MCF-7 cells (p < 0.05).
34973989	8	13	theme	MCF-7	1373:1377	arg1	cells					1379:1383	MCF-7 cells	1373:1383	MCF-7 cells (p < 0.05)	1373:1394	However, 5-Fluorouracil@MIL-100 and chitosan-coated 5-Fluorouracil@MIL-100 with quercetin were more effective than other nanocarriers in MCF-7 cells (p < 0.05).
34973989	0	14	theme	biocompatible	130:142	arg1	composite					104:112	chitosan-coated MIL-100 composite	80:112	chitosan-coated MIL-100 composite	80:112	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	0	14	theme	biocompatible	130:142	arg1	system					182:187	an advanced, biocompatible, pH-sensitive and smart drug delivery system	117:187	an advanced, biocompatible, pH-sensitive and smart drug delivery system	117:187	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	11	15	theme	normal	1774:1779	arg1	cells					1781:1785	normal cells	1774:1785	normal cells	1774:1785	Therefore, MIL-100 could be used for a promising chemotherapeutic drugs delivery and chitosan coated drugs with quercetin could be useful for reducing toxicity on normal cells.
34973989	8	16	from	cells	1379:1383	arg1	effective					1336:1344	effective	1336:1344	effective	1336:1344	However, 5-Fluorouracil@MIL-100 and chitosan-coated 5-Fluorouracil@MIL-100 with quercetin were more effective than other nanocarriers in MCF-7 cells (p < 0.05).
34973989	5	17	theme	MIL-100	931:937	arg1	stability					918:926	the inherent pH-dependent stability	892:926	the inherent pH-dependent stability of MIL-100	892:937	pH-sensitive releases of 5-Fluorouracil and GEM were observed because of the inherent pH-dependent stability of MIL-100.
34973989	11	18	with	drugs	1712:1716	arg1	quercetin					1723:1731	quercetin	1723:1731	quercetin	1723:1731	Therefore, MIL-100 could be used for a promising chemotherapeutic drugs delivery and chitosan coated drugs with quercetin could be useful for reducing toxicity on normal cells.
34973989	5	19	theme	5-Fluorouracil	844:857	arg1	pH-sensitive					819:830	pH-sensitive	819:830	pH-sensitive	819:830	pH-sensitive releases of 5-Fluorouracil and GEM were observed because of the inherent pH-dependent stability of MIL-100.
34973989	10	20	theme	chitosan	1511:1518	arg1	nanocarriers					1527:1538	chitosan coated nanocarriers	1511:1538	chitosan coated nanocarriers with quercetin	1511:1553	However, chitosan coated nanocarriers with quercetin were less toxic on HUVEC cells at particularly 1 µg/mL.
34973989	9	21	from	cytotoxicity	1419:1430	arg1	cells					1441:1445	HUVEC cells	1435:1445	HUVEC cells	1435:1445	Moreover, we observed cytotoxicity in HUVEC cells due to the adverse side effects of chemotherapy drugs.
34973989	9	22	theme	adverse	1458:1464	arg1	effects					1471:1477	the adverse side effects	1454:1477	the adverse side effects of chemotherapy drugs	1454:1499	Moreover, we observed cytotoxicity in HUVEC cells due to the adverse side effects of chemotherapy drugs.
34973989	6	23	theme	nanocarriers	1001:1012	arg1	activity					983:990	the anti-cancer activity	967:990	the anti-cancer activity of these nanocarriers	967:1012	Additionally, we evaluated the anti-cancer activity of these nanocarriers through WST-1 analysis and acridine orange staining in MCF-7 human breast cancer and HUVEC control cell lines.
34973989	8	24	theme	5-Fluorouracil	1288:1301	arg1	MIL-100					1303:1309	chitosan-coated 5-Fluorouracil@MIL-100	1272:1309	chitosan-coated 5-Fluorouracil@MIL-100	1272:1309	However, 5-Fluorouracil@MIL-100 and chitosan-coated 5-Fluorouracil@MIL-100 with quercetin were more effective than other nanocarriers in MCF-7 cells (p < 0.05).
34973989	6	25	theme	acridine	1041:1048	arg1	staining					1057:1064	acridine orange staining	1041:1064	acridine orange staining	1041:1064	Additionally, we evaluated the anti-cancer activity of these nanocarriers through WST-1 analysis and acridine orange staining in MCF-7 human breast cancer and HUVEC control cell lines.
34973989	8	26	with	MIL-100	1260:1266	arg1	quercetin					1316:1324	quercetin	1316:1324	quercetin	1316:1324	However, 5-Fluorouracil@MIL-100 and chitosan-coated 5-Fluorouracil@MIL-100 with quercetin were more effective than other nanocarriers in MCF-7 cells (p < 0.05).
34973989	4	27	theme	different	783:791	arg1	results					810:816	different characterization results	783:816	different characterization results	783:816	The hybrid nanocarrier nascency was verified by different characterization results.
34973989	8	28	theme	chitosan-coated	1272:1286	arg1	MIL-100					1303:1309	chitosan-coated 5-Fluorouracil@MIL-100	1272:1309	chitosan-coated 5-Fluorouracil@MIL-100	1272:1309	However, 5-Fluorouracil@MIL-100 and chitosan-coated 5-Fluorouracil@MIL-100 with quercetin were more effective than other nanocarriers in MCF-7 cells (p < 0.05).
34973989	1	29	theme	Gemcitabine	298:308	arg1	efficiency					284:293	the bioavailability and therapeutic efficiency	248:293	the bioavailability and therapeutic efficiency of Gemcitabine (GEM) and 5-Fluorouracil with decreased side effects using MIL-100 nano-composite as carrier	248:401	This study was planned to evolve the bioavailability and therapeutic efficiency of Gemcitabine (GEM) and 5-Fluorouracil with decreased side effects using MIL-100 nano-composite as carrier.
34973989	7	30	theme	anti-cancer	1177:1187	arg1	activity					1189:1196	anti-cancer activity	1177:1196	anti-cancer activity	1177:1196	Our findings showed that all nanocarriers exhibited anti-cancer activity and induced apoptosis in MCF-7 cells.
34973989	7	31	from	apoptosis	1210:1218	arg1	cells					1229:1233	MCF-7 cells	1223:1233	MCF-7 cells	1223:1233	Our findings showed that all nanocarriers exhibited anti-cancer activity and induced apoptosis in MCF-7 cells.
34973989	2	32	theme	Impregnation	404:415	arg1	approach					417:424	Impregnation approach	404:424	Impregnation approach	404:424	Impregnation approach was used for encapsulation of 5-Fluorouracil alone and with GEM inside the MIL-100.
34973989	9	33	theme	HUVEC	1435:1439	arg1	cells					1441:1445	HUVEC cells	1435:1445	HUVEC cells	1435:1445	Moreover, we observed cytotoxicity in HUVEC cells due to the adverse side effects of chemotherapy drugs.
34973989	0	34	theme	pH-sensitive	145:156	arg1	composite					104:112	chitosan-coated MIL-100 composite	80:112	chitosan-coated MIL-100 composite	80:112	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	0	34	theme	pH-sensitive	145:156	arg1	system					182:187	an advanced, biocompatible, pH-sensitive and smart drug delivery system	117:187	an advanced, biocompatible, pH-sensitive and smart drug delivery system	117:187	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	2	35	theme	5-Fluorouracil	456:469	arg1	encapsulation					439:451	encapsulation	439:451	encapsulation of 5-Fluorouracil alone	439:475	Impregnation approach was used for encapsulation of 5-Fluorouracil alone and with GEM inside the MIL-100.
34973989	0	36	theme	5-Fluorouracil	17:30	arg1	Effectiveness					0:12	Effectiveness	0:12	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded	0:67	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	6	37	theme	orange	1050:1055	arg1	staining					1057:1064	acridine orange staining	1041:1064	acridine orange staining	1041:1064	Additionally, we evaluated the anti-cancer activity of these nanocarriers through WST-1 analysis and acridine orange staining in MCF-7 human breast cancer and HUVEC control cell lines.
34973989	11	38	theme	drugs	1677:1681	arg1	delivery					1683:1690	a promising chemotherapeutic drugs delivery	1648:1690	a promising chemotherapeutic drugs delivery	1648:1690	Therefore, MIL-100 could be used for a promising chemotherapeutic drugs delivery and chitosan coated drugs with quercetin could be useful for reducing toxicity on normal cells.
34973989	4	39	theme	characterization	793:808	arg1	results					810:816	different characterization results	783:816	different characterization results	783:816	The hybrid nanocarrier nascency was verified by different characterization results.
34973989	8	40	theme	@	1259:1259	arg1	MIL-100					1260:1266	5-Fluorouracil@MIL-100	1245:1266	5-Fluorouracil@MIL-100	1245:1266	However, 5-Fluorouracil@MIL-100 and chitosan-coated 5-Fluorouracil@MIL-100 with quercetin were more effective than other nanocarriers in MCF-7 cells (p < 0.05).
34973989	5	41	theme	pH-dependent	905:916	arg1	stability					918:926	the inherent pH-dependent stability	892:926	the inherent pH-dependent stability of MIL-100	892:937	pH-sensitive releases of 5-Fluorouracil and GEM were observed because of the inherent pH-dependent stability of MIL-100.
34973989	0	42	theme	hydrochloride	48:60	arg1	Effectiveness					0:12	Effectiveness	0:12	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded	0:67	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	4	43	theme	nanocarrier	746:756	arg1	nascency					758:765	The hybrid nanocarrier nascency	735:765	The hybrid nanocarrier nascency	735:765	The hybrid nanocarrier nascency was verified by different characterization results.
34973989	0	44	theme	smart	162:166	arg1	composite					104:112	chitosan-coated MIL-100 composite	80:112	chitosan-coated MIL-100 composite	80:112	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	0	44	theme	smart	162:166	arg1	system					182:187	an advanced, biocompatible, pH-sensitive and smart drug delivery system	117:187	an advanced, biocompatible, pH-sensitive and smart drug delivery system	117:187	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	8	45	theme	other	1351:1355	arg1	nanocarriers					1357:1368	other nanocarriers	1351:1368	other nanocarriers	1351:1368	However, 5-Fluorouracil@MIL-100 and chitosan-coated 5-Fluorouracil@MIL-100 with quercetin were more effective than other nanocarriers in MCF-7 cells (p < 0.05).
34973989	0	46	theme	gemcitabine	36:46	arg1	hydrochloride					48:60	gemcitabine hydrochloride	36:60	gemcitabine hydrochloride loaded	36:67	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	5	47	dep	pH-sensitive	819:830	arg1	releases					832:839	releases	832:839	releases	832:839	pH-sensitive releases of 5-Fluorouracil and GEM were observed because of the inherent pH-dependent stability of MIL-100.
34973989	6	48	theme	breast	1081:1086	arg1	cancer					1088:1093	human breast cancer	1075:1093	human breast cancer	1075:1093	Additionally, we evaluated the anti-cancer activity of these nanocarriers through WST-1 analysis and acridine orange staining in MCF-7 human breast cancer and HUVEC control cell lines.
34973989	9	49	theme	chemotherapy	1482:1493	arg1	drugs					1495:1499	chemotherapy drugs	1482:1499	chemotherapy drugs	1482:1499	Moreover, we observed cytotoxicity in HUVEC cells due to the adverse side effects of chemotherapy drugs.
34973989	1	50	with	efficiency	284:293	arg1	effects					355:361	decreased side effects	340:361	decreased side effects using MIL-100 nano-composite as carrier	340:401	This study was planned to evolve the bioavailability and therapeutic efficiency of Gemcitabine (GEM) and 5-Fluorouracil with decreased side effects using MIL-100 nano-composite as carrier.
34973989	9	51	theme	side	1466:1469	arg1	effects					1471:1477	the adverse side effects	1454:1477	the adverse side effects of chemotherapy drugs	1454:1499	Moreover, we observed cytotoxicity in HUVEC cells due to the adverse side effects of chemotherapy drugs.
34973989	5	52	theme	GEM	863:865	arg1	pH-sensitive					819:830	pH-sensitive	819:830	pH-sensitive	819:830	pH-sensitive releases of 5-Fluorouracil and GEM were observed because of the inherent pH-dependent stability of MIL-100.
34973989	1	53	theme	5-Fluorouracil	320:333	arg1	efficiency					284:293	the bioavailability and therapeutic efficiency	248:293	the bioavailability and therapeutic efficiency of Gemcitabine (GEM) and 5-Fluorouracil with decreased side effects using MIL-100 nano-composite as carrier	248:401	This study was planned to evolve the bioavailability and therapeutic efficiency of Gemcitabine (GEM) and 5-Fluorouracil with decreased side effects using MIL-100 nano-composite as carrier.
34973989	0	54	theme	delivery	173:180	arg1	composite					104:112	chitosan-coated MIL-100 composite	80:112	chitosan-coated MIL-100 composite	80:112	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	0	54	theme	delivery	173:180	arg1	system					182:187	an advanced, biocompatible, pH-sensitive and smart drug delivery system	117:187	an advanced, biocompatible, pH-sensitive and smart drug delivery system	117:187	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	11	55	theme	chitosan	1696:1703	arg1	drugs					1712:1716	chitosan coated drugs	1696:1716	chitosan coated drugs with quercetin	1696:1731	Therefore, MIL-100 could be used for a promising chemotherapeutic drugs delivery and chitosan coated drugs with quercetin could be useful for reducing toxicity on normal cells.
34973989	3	56	theme	5-Fluorouracil	521:534	arg1	MIL-100					536:542	The formed 5-Fluorouracil@MIL-100	510:542	The formed 5-Fluorouracil@MIL-100	510:542	The formed 5-Fluorouracil@MIL-100 and 5-Fluorouracil-GEM@MIL-100 were then coated with chitosan, sequentially chelated with iron(III) and conjugated with quercetin, eventually obtaining a multifunctional MIL-100 nanocarrier.
34973989	10	57	theme	HUVEC	1574:1578	arg1	cells					1580:1584	HUVEC cells	1574:1584	HUVEC cells	1574:1584	However, chitosan coated nanocarriers with quercetin were less toxic on HUVEC cells at particularly 1 µg/mL.
34973989	6	58	theme	WST-1	1022:1026	arg1	analysis					1028:1035	WST-1 analysis	1022:1035	WST-1 analysis	1022:1035	Additionally, we evaluated the anti-cancer activity of these nanocarriers through WST-1 analysis and acridine orange staining in MCF-7 human breast cancer and HUVEC control cell lines.
34973989	0	59	theme	drug	168:171	arg1	composite					104:112	chitosan-coated MIL-100 composite	80:112	chitosan-coated MIL-100 composite	80:112	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	0	59	theme	drug	168:171	arg1	system					182:187	an advanced, biocompatible, pH-sensitive and smart drug delivery system	117:187	an advanced, biocompatible, pH-sensitive and smart drug delivery system	117:187	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	3	60	theme	multifunctional	698:712	arg1	nanocarrier					722:732	a multifunctional MIL-100 nanocarrier	696:732	a multifunctional MIL-100 nanocarrier	696:732	The formed 5-Fluorouracil@MIL-100 and 5-Fluorouracil-GEM@MIL-100 were then coated with chitosan, sequentially chelated with iron(III) and conjugated with quercetin, eventually obtaining a multifunctional MIL-100 nanocarrier.
34973989	7	61	theme	MCF-7	1223:1227	arg1	cells					1229:1233	MCF-7 cells	1223:1233	MCF-7 cells	1223:1233	Our findings showed that all nanocarriers exhibited anti-cancer activity and induced apoptosis in MCF-7 cells.
34973989	10	62	from	1 µg/mL	1602:1608	arg1	toxic					1565:1569	toxic	1565:1569	toxic	1565:1569	However, chitosan coated nanocarriers with quercetin were less toxic on HUVEC cells at particularly 1 µg/mL.
34973989	3	63	theme	@	535:535	arg1	MIL-100					536:542	The formed 5-Fluorouracil@MIL-100	510:542	The formed 5-Fluorouracil@MIL-100	510:542	The formed 5-Fluorouracil@MIL-100 and 5-Fluorouracil-GEM@MIL-100 were then coated with chitosan, sequentially chelated with iron(III) and conjugated with quercetin, eventually obtaining a multifunctional MIL-100 nanocarrier.
34973989	1	64	theme	decreased	340:348	arg1	effects					355:361	decreased side effects	340:361	decreased side effects using MIL-100 nano-composite as carrier	340:401	This study was planned to evolve the bioavailability and therapeutic efficiency of Gemcitabine (GEM) and 5-Fluorouracil with decreased side effects using MIL-100 nano-composite as carrier.
34973989	11	65	theme	promising	1650:1658	arg1	drugs					1677:1681	promising chemotherapeutic drugs	1650:1681	a promising chemotherapeutic drugs delivery	1648:1690	Therefore, MIL-100 could be used for a promising chemotherapeutic drugs delivery and chitosan coated drugs with quercetin could be useful for reducing toxicity on normal cells.
34973989	3	66	theme	MIL-100	714:720	arg1	nanocarrier					722:732	a multifunctional MIL-100 nanocarrier	696:732	a multifunctional MIL-100 nanocarrier	696:732	The formed 5-Fluorouracil@MIL-100 and 5-Fluorouracil-GEM@MIL-100 were then coated with chitosan, sequentially chelated with iron(III) and conjugated with quercetin, eventually obtaining a multifunctional MIL-100 nanocarrier.
34973989	8	67	from	effective	1336:1344	arg1	p < 0.05					1386:1393	p < 0.05	1386:1393	p < 0.05	1386:1393	However, 5-Fluorouracil@MIL-100 and chitosan-coated 5-Fluorouracil@MIL-100 with quercetin were more effective than other nanocarriers in MCF-7 cells (p < 0.05).
34973989	8	67	from	effective	1336:1344	arg1	cells					1379:1383	MCF-7 cells	1373:1383	MCF-7 cells (p < 0.05)	1373:1394	However, 5-Fluorouracil@MIL-100 and chitosan-coated 5-Fluorouracil@MIL-100 with quercetin were more effective than other nanocarriers in MCF-7 cells (p < 0.05).
34973989	11	68	used	used	1639:1642	arg2	MIL-100					1622:1628	MIL-100	1622:1628	MIL-100	1622:1628	Therefore, MIL-100 could be used for a promising chemotherapeutic drugs delivery and chitosan coated drugs with quercetin could be useful for reducing toxicity on normal cells.
34973989	6	69	theme	cell	1113:1116	arg1	lines					1118:1122	HUVEC control cell lines	1099:1122	HUVEC control cell lines	1099:1122	Additionally, we evaluated the anti-cancer activity of these nanocarriers through WST-1 analysis and acridine orange staining in MCF-7 human breast cancer and HUVEC control cell lines.
34973989	8	70	with	MIL-100	1303:1309	arg1	quercetin					1316:1324	quercetin	1316:1324	quercetin	1316:1324	However, 5-Fluorouracil@MIL-100 and chitosan-coated 5-Fluorouracil@MIL-100 with quercetin were more effective than other nanocarriers in MCF-7 cells (p < 0.05).
34973989	3	71	theme	formed	514:519	arg1	MIL-100					536:542	The formed 5-Fluorouracil@MIL-100	510:542	The formed 5-Fluorouracil@MIL-100	510:542	The formed 5-Fluorouracil@MIL-100 and 5-Fluorouracil-GEM@MIL-100 were then coated with chitosan, sequentially chelated with iron(III) and conjugated with quercetin, eventually obtaining a multifunctional MIL-100 nanocarrier.
34973989	6	72	theme	control	1105:1111	arg1	lines					1118:1122	HUVEC control cell lines	1099:1122	HUVEC control cell lines	1099:1122	Additionally, we evaluated the anti-cancer activity of these nanocarriers through WST-1 analysis and acridine orange staining in MCF-7 human breast cancer and HUVEC control cell lines.
34973989	0	73	theme	MIL-100	96:102	arg1	composite					104:112	chitosan-coated MIL-100 composite	80:112	chitosan-coated MIL-100 composite	80:112	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	0	73	theme	MIL-100	96:102	arg1	system					182:187	an advanced, biocompatible, pH-sensitive and smart drug delivery system	117:187	an advanced, biocompatible, pH-sensitive and smart drug delivery system	117:187	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	5	74	theme	inherent	896:903	arg1	stability					918:926	the inherent pH-dependent stability	892:926	the inherent pH-dependent stability of MIL-100	892:937	pH-sensitive releases of 5-Fluorouracil and GEM were observed because of the inherent pH-dependent stability of MIL-100.
34973989	3	75	theme	5-Fluorouracil-GEM	548:565	arg1	MIL-100					567:573	5-Fluorouracil-GEM@MIL-100	548:573	5-Fluorouracil-GEM@MIL-100	548:573	The formed 5-Fluorouracil@MIL-100 and 5-Fluorouracil-GEM@MIL-100 were then coated with chitosan, sequentially chelated with iron(III) and conjugated with quercetin, eventually obtaining a multifunctional MIL-100 nanocarrier.
34973989	6	76	theme	HUVEC	1099:1103	arg1	lines					1118:1122	HUVEC control cell lines	1099:1122	HUVEC control cell lines	1099:1122	Additionally, we evaluated the anti-cancer activity of these nanocarriers through WST-1 analysis and acridine orange staining in MCF-7 human breast cancer and HUVEC control cell lines.
34973989	0	77	theme	chitosan-coated	80:94	arg1	composite					104:112	chitosan-coated MIL-100 composite	80:112	chitosan-coated MIL-100 composite	80:112	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	0	77	theme	chitosan-coated	80:94	arg1	system					182:187	an advanced, biocompatible, pH-sensitive and smart drug delivery system	117:187	an advanced, biocompatible, pH-sensitive and smart drug delivery system	117:187	Effectiveness of 5-Fluorouracil and gemcitabine hydrochloride loaded iron‑based chitosan-coated MIL-100 composite as an advanced, biocompatible, pH-sensitive and smart drug delivery system on breast cancer therapy.
34973989	1	78	theme	side	350:353	arg1	effects					355:361	decreased side effects	340:361	decreased side effects using MIL-100 nano-composite as carrier	340:401	This study was planned to evolve the bioavailability and therapeutic efficiency of Gemcitabine (GEM) and 5-Fluorouracil with decreased side effects using MIL-100 nano-composite as carrier.
34973989	8	79	theme	@	1302:1302	arg1	MIL-100					1303:1309	chitosan-coated 5-Fluorouracil@MIL-100	1272:1309	chitosan-coated 5-Fluorouracil@MIL-100	1272:1309	However, 5-Fluorouracil@MIL-100 and chitosan-coated 5-Fluorouracil@MIL-100 with quercetin were more effective than other nanocarriers in MCF-7 cells (p < 0.05).
34973989	11	80	theme	coated	1705:1710	arg1	drugs					1712:1716	chitosan coated drugs	1696:1716	chitosan coated drugs with quercetin	1696:1731	Therefore, MIL-100 could be used for a promising chemotherapeutic drugs delivery and chitosan coated drugs with quercetin could be useful for reducing toxicity on normal cells.
34973989	3	81	theme	@	566:566	arg1	MIL-100					567:573	5-Fluorouracil-GEM@MIL-100	548:573	5-Fluorouracil-GEM@MIL-100	548:573	The formed 5-Fluorouracil@MIL-100 and 5-Fluorouracil-GEM@MIL-100 were then coated with chitosan, sequentially chelated with iron(III) and conjugated with quercetin, eventually obtaining a multifunctional MIL-100 nanocarrier.
34973989	9	82	theme	drugs	1495:1499	arg1	effects					1471:1477	the adverse side effects	1454:1477	the adverse side effects of chemotherapy drugs	1454:1499	Moreover, we observed cytotoxicity in HUVEC cells due to the adverse side effects of chemotherapy drugs.
34973989	1	83	theme	bioavailability	252:266	arg1	efficiency					284:293	the bioavailability and therapeutic efficiency	248:293	the bioavailability and therapeutic efficiency of Gemcitabine (GEM) and 5-Fluorouracil with decreased side effects using MIL-100 nano-composite as carrier	248:401	This study was planned to evolve the bioavailability and therapeutic efficiency of Gemcitabine (GEM) and 5-Fluorouracil with decreased side effects using MIL-100 nano-composite as carrier.
32409038	10	0	theme	bioactive	1628:1636	arg1	compound					1638:1645	the bioactive compound	1624:1645	the bioactive compound above 87%	1624:1655	Incorporation efficiencies of the bioactive compound above 87% were achieved, and the addition of P and S to the membrane formulation changed the release of the A. chica extract kinetics.
32409038	6	1	theme	obtaining	1109:1117	arg1	matrices					1126:1133	obtaining porous matrices	1109:1133	obtaining porous matrices	1109:1133	The results show that the inclusion of P in the formulation allows obtaining porous matrices, promotes greater homogeneity of the mixture of the silicone gel with the suspension of polysaccharides, and increases the swelling of the polymer matrix.
32409038	4	2	contain	have	644:647	arg1	matrices					610:617	composite matrices	600:617	composite matrices of these polysaccharides	600:642	Furthermore, composite matrices of these polysaccharides have potential applications as devices for releasing bioactive compounds to skin lesions.
32409038	4	2	contain	have	644:647	arg2	applications					659:670	potential applications	649:670	potential applications as devices	649:681	Furthermore, composite matrices of these polysaccharides have potential applications as devices for releasing bioactive compounds to skin lesions.
32409038	6	3	theme	gel	1196:1198	arg1	mixture					1172:1178	the mixture	1168:1178	the mixture of the silicone gel with the suspension of polysaccharides	1168:1237	The results show that the inclusion of P in the formulation allows obtaining porous matrices, promotes greater homogeneity of the mixture of the silicone gel with the suspension of polysaccharides, and increases the swelling of the polymer matrix.
32409038	10	4	theme	compound	1638:1645	arg1	efficiencies					1608:1619	Incorporation efficiencies	1594:1619	Incorporation efficiencies of the bioactive compound above 87%	1594:1655	Incorporation efficiencies of the bioactive compound above 87% were achieved, and the addition of P and S to the membrane formulation changed the release of the A. chica extract kinetics.
32409038	7	5	theme	maximum	1344:1350	arg1	loss					1357:1360	a maximum mass loss	1342:1360	a maximum mass loss of 18%	1342:1367	All formulations presented high stability, reaching a maximum mass loss of 18% after seven days.
32409038	6	6	gly	homogeneity	1153:1163	arg1	mixture					1172:1178	the mixture	1168:1178	the mixture of the silicone gel with the suspension of polysaccharides	1168:1237	The results show that the inclusion of P in the formulation allows obtaining porous matrices, promotes greater homogeneity of the mixture of the silicone gel with the suspension of polysaccharides, and increases the swelling of the polymer matrix.
32409038	2	7	with	structure	348:356	arg1	characteristics					364:378	characteristics	364:378	characteristics tunable for different applications	364:413	CA films can show a dense or porous flexible structure, with characteristics tunable for different applications.
32409038	9	8	theme	membranes	1583:1591	arg1	characteristics					1560:1574	the characteristics	1556:1574	the characteristics of the membranes	1556:1591	The presence of A. chica standardized extract did not affect negatively the characteristics of the membranes.
32409038	6	9	theme	mixture	1172:1178	arg1	homogeneity					1153:1163	greater homogeneity	1145:1163	greater homogeneity of the mixture of the silicone gel with the suspension of polysaccharides	1145:1237	The results show that the inclusion of P in the formulation allows obtaining porous matrices, promotes greater homogeneity of the mixture of the silicone gel with the suspension of polysaccharides, and increases the swelling of the polymer matrix.
32409038	3	10	theme	Kolliphor®	511:520	arg1	P					528:528	P	528:528	P	528:528	Porosity and flexibility can be achieved, respectively, by the addition of surfactants such as Kolliphor® P188 (P) and silicone-based compounds as Silpuran® 2130 A/B (S).
32409038	3	10	theme	Kolliphor®	511:520	arg1	P188					522:525	Kolliphor® P188	511:525	Kolliphor® P188 (P)	511:529	Porosity and flexibility can be achieved, respectively, by the addition of surfactants such as Kolliphor® P188 (P) and silicone-based compounds as Silpuran® 2130 A/B (S).
32409038	1	11	theme	chitosan	176:183	arg1	combination					161:171	The combination	157:171	The combination of chitosan (C) with alginate (A)	157:205	The combination of chitosan (C) with alginate (A) has been explored for the production of dressings due to the positive results on wound healing.
32409038	0	12	theme	Verlot	115:120	arg1	extract					87:93	the standardized extract	70:93	the standardized extract of Arrabidaea chica Verlot for the treatment of skin lesions	70:154	Flexible, dense and porous chitosan and alginate membranes containing the standardized extract of Arrabidaea chica Verlot for the treatment of skin lesions.
32409038	10	13	theme	S	1698:1698	arg1	addition					1680:1687	the addition	1676:1687	the addition of P and S to the membrane formulation	1676:1726	Incorporation efficiencies of the bioactive compound above 87% were achieved, and the addition of P and S to the membrane formulation changed the release of the A. chica extract kinetics.
32409038	4	14	theme	polysaccharides	628:642	arg1	matrices					610:617	composite matrices	600:617	composite matrices of these polysaccharides	600:642	Furthermore, composite matrices of these polysaccharides have potential applications as devices for releasing bioactive compounds to skin lesions.
32409038	10	15	theme	P	1692:1692	arg1	addition					1680:1687	the addition	1676:1687	the addition of P and S to the membrane formulation	1676:1726	Incorporation efficiencies of the bioactive compound above 87% were achieved, and the addition of P and S to the membrane formulation changed the release of the A. chica extract kinetics.
32409038	5	16	theme	CA	856:857	arg1	membranes					859:867	flexible dense and porous CA membranes	830:867	flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica)	830:944	The purpose of this study was to evaluate the physicochemical and biological characteristics of flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica), and also to analyze the release mechanism of the extract from different membrane formulations.
32409038	4	17	theme	potential	649:657	arg1	applications					659:670	potential applications	649:670	potential applications as devices	649:681	Furthermore, composite matrices of these polysaccharides have potential applications as devices for releasing bioactive compounds to skin lesions.
32409038	0	18	theme	lesions	148:154	arg1	treatment					130:138	the treatment	126:138	the treatment of skin lesions	126:154	Flexible, dense and porous chitosan and alginate membranes containing the standardized extract of Arrabidaea chica Verlot for the treatment of skin lesions.
32409038	5	19	theme	study	754:758	arg1	purpose					738:744	The purpose	734:744	The purpose of this study	734:758	The purpose of this study was to evaluate the physicochemical and biological characteristics of flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica), and also to analyze the release mechanism of the extract from different membrane formulations.
32409038	4	20	theme	composite	600:608	arg1	matrices					610:617	composite matrices	600:617	composite matrices of these polysaccharides	600:642	Furthermore, composite matrices of these polysaccharides have potential applications as devices for releasing bioactive compounds to skin lesions.
32409038	0	21	theme	dense	10:14	arg1	membranes					49:57	Flexible, dense and porous chitosan and alginate membranes	0:57	Flexible, dense and porous chitosan and alginate membranes	0:57	Flexible, dense and porous chitosan and alginate membranes containing the standardized extract of Arrabidaea chica Verlot for the treatment of skin lesions.
32409038	5	22	theme	flexible	830:837	arg1	membranes					859:867	flexible dense and porous CA membranes	830:867	flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica)	830:944	The purpose of this study was to evaluate the physicochemical and biological characteristics of flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica), and also to analyze the release mechanism of the extract from different membrane formulations.
32409038	1	23	theme	wound	288:292	arg1	healing					294:300	wound healing	288:300	wound healing	288:300	The combination of chitosan (C) with alginate (A) has been explored for the production of dressings due to the positive results on wound healing.
32409038	4	24	theme	bioactive	697:705	arg1	compounds					707:715	bioactive compounds	697:715	bioactive compounds	697:715	Furthermore, composite matrices of these polysaccharides have potential applications as devices for releasing bioactive compounds to skin lesions.
32409038	6	25	theme	matrix	1282:1287	arg1	swelling					1258:1265	the swelling	1254:1265	the swelling of the polymer matrix	1254:1287	The results show that the inclusion of P in the formulation allows obtaining porous matrices, promotes greater homogeneity of the mixture of the silicone gel with the suspension of polysaccharides, and increases the swelling of the polymer matrix.
32409038	5	26	theme	membrane	1019:1026	arg1	formulations					1028:1039	different membrane formulations	1009:1039	different membrane formulations	1009:1039	The purpose of this study was to evaluate the physicochemical and biological characteristics of flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica), and also to analyze the release mechanism of the extract from different membrane formulations.
32409038	8	27	theme	best	1422:1425	arg1	performance					1427:1437	the best performance	1418:1437	the best performance	1418:1437	The formulations with S showed the best performance in terms of flexibility and strain at break.
32409038	11	28	theme	developed	1799:1807	arg1	formulations					1809:1820	the developed formulations	1795:1820	the developed formulations	1795:1820	In addition, the developed formulations did not significantly affect Vero cells proliferation.
32409038	0	29	theme	Flexible	0:7	arg1	membranes					49:57	Flexible, dense and porous chitosan and alginate membranes	0:57	Flexible, dense and porous chitosan and alginate membranes	0:57	Flexible, dense and porous chitosan and alginate membranes containing the standardized extract of Arrabidaea chica Verlot for the treatment of skin lesions.
32409038	5	30	theme	standardized	887:898	arg1	extract					900:906	the standardized extract	883:906	the standardized extract of Arrabidaea chica Verlot (A. chica)	883:944	The purpose of this study was to evaluate the physicochemical and biological characteristics of flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica), and also to analyze the release mechanism of the extract from different membrane formulations.
32409038	11	31	theme	cells	1856:1860	arg1	proliferation					1862:1874	Vero cells proliferation	1851:1874	Vero cells proliferation	1851:1874	In addition, the developed formulations did not significantly affect Vero cells proliferation.
32409038	6	32	from	inclusion	1068:1076	arg1	formulation					1090:1100	the formulation	1086:1100	the formulation	1086:1100	The results show that the inclusion of P in the formulation allows obtaining porous matrices, promotes greater homogeneity of the mixture of the silicone gel with the suspension of polysaccharides, and increases the swelling of the polymer matrix.
32409038	5	33	from	formulations	1028:1039	arg1	mechanism					979:987	the release mechanism	967:987	the release mechanism of the extract from different membrane formulations	967:1039	The purpose of this study was to evaluate the physicochemical and biological characteristics of flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica), and also to analyze the release mechanism of the extract from different membrane formulations.
32409038	5	33	from	formulations	1028:1039	arg1	extract					996:1002	the extract	992:1002	the extract from different membrane formulations	992:1039	The purpose of this study was to evaluate the physicochemical and biological characteristics of flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica), and also to analyze the release mechanism of the extract from different membrane formulations.
32409038	2	34	theme	flexible	339:346	arg1	structure					348:356	a dense or porous flexible structure	321:356	a dense or porous flexible structure	321:356	CA films can show a dense or porous flexible structure, with characteristics tunable for different applications.
32409038	0	35	theme	porous	20:25	arg1	membranes					49:57	Flexible, dense and porous chitosan and alginate membranes	0:57	Flexible, dense and porous chitosan and alginate membranes	0:57	Flexible, dense and porous chitosan and alginate membranes containing the standardized extract of Arrabidaea chica Verlot for the treatment of skin lesions.
32409038	1	36	theme	due	257:259	arg1	dressings					247:255	dressings	247:255	dressings due to the positive results on wound healing	247:300	The combination of chitosan (C) with alginate (A) has been explored for the production of dressings due to the positive results on wound healing.
32409038	3	37	theme	2130	573:576	arg1	S					583:583	Silpuran® 2130 A/B (S)	563:584	Silpuran® 2130 A/B (S)	563:584	Porosity and flexibility can be achieved, respectively, by the addition of surfactants such as Kolliphor® P188 (P) and silicone-based compounds as Silpuran® 2130 A/B (S).
32409038	9	38	theme	chica	1503:1507	arg1	extract					1522:1528	A. chica standardized extract	1500:1528	A. chica standardized extract	1500:1528	The presence of A. chica standardized extract did not affect negatively the characteristics of the membranes.
32409038	3	39	theme	silicone-based	535:548	arg1	compounds					550:558	silicone-based compounds	535:558	silicone-based compounds as Silpuran® 2130 A/B (S)	535:584	Porosity and flexibility can be achieved, respectively, by the addition of surfactants such as Kolliphor® P188 (P) and silicone-based compounds as Silpuran® 2130 A/B (S).
32409038	10	40	theme	extract	1764:1770	arg1	kinetics					1772:1779	the A. chica extract kinetics	1751:1779	the A. chica extract kinetics	1751:1779	Incorporation efficiencies of the bioactive compound above 87% were achieved, and the addition of P and S to the membrane formulation changed the release of the A. chica extract kinetics.
32409038	1	41	with	combination	161:171	arg1	A					204:204	A	204:204	A	204:204	The combination of chitosan (C) with alginate (A) has been explored for the production of dressings due to the positive results on wound healing.
32409038	1	41	with	combination	161:171	arg1	alginate					194:201	alginate	194:201	alginate (A)	194:205	The combination of chitosan (C) with alginate (A) has been explored for the production of dressings due to the positive results on wound healing.
32409038	9	42	theme	extract	1522:1528	arg1	presence					1488:1495	The presence	1484:1495	The presence of A. chica standardized extract	1484:1528	The presence of A. chica standardized extract did not affect negatively the characteristics of the membranes.
32409038	10	43	dep	A.	1755:1756	arg1	chica					1758:1762	chica	1758:1762	chica	1758:1762	Incorporation efficiencies of the bioactive compound above 87% were achieved, and the addition of P and S to the membrane formulation changed the release of the A. chica extract kinetics.
32409038	10	44	theme	A.	1755:1756	arg1	kinetics					1772:1779	the A. chica extract kinetics	1751:1779	the A. chica extract kinetics	1751:1779	Incorporation efficiencies of the bioactive compound above 87% were achieved, and the addition of P and S to the membrane formulation changed the release of the A. chica extract kinetics.
32409038	8	45	with	formulations	1391:1402	arg1	S					1409:1409	S	1409:1409	S	1409:1409	The formulations with S showed the best performance in terms of flexibility and strain at break.
32409038	1	46	from	results	277:283	arg1	healing					294:300	wound healing	288:300	wound healing	288:300	The combination of chitosan (C) with alginate (A) has been explored for the production of dressings due to the positive results on wound healing.
32409038	7	47	theme	%	1367:1367	arg1	loss					1357:1360	a maximum mass loss	1342:1360	a maximum mass loss of 18%	1342:1367	All formulations presented high stability, reaching a maximum mass loss of 18% after seven days.
32409038	2	48	theme	CA	303:304	arg1	films					306:310	CA films	303:310	CA films	303:310	CA films can show a dense or porous flexible structure, with characteristics tunable for different applications.
32409038	6	49	with	mixture	1172:1178	arg1	suspension					1209:1218	the suspension	1205:1218	the suspension of polysaccharides	1205:1237	The results show that the inclusion of P in the formulation allows obtaining porous matrices, promotes greater homogeneity of the mixture of the silicone gel with the suspension of polysaccharides, and increases the swelling of the polymer matrix.
32409038	6	50	theme	silicone	1187:1194	arg1	gel					1196:1198	the silicone gel	1183:1198	the silicone gel	1183:1198	The results show that the inclusion of P in the formulation allows obtaining porous matrices, promotes greater homogeneity of the mixture of the silicone gel with the suspension of polysaccharides, and increases the swelling of the polymer matrix.
32409038	5	51	theme	physicochemical	780:794	arg1	characteristics					811:825	the physicochemical and biological characteristics	776:825	the physicochemical and biological characteristics of flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica)	776:944	The purpose of this study was to evaluate the physicochemical and biological characteristics of flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica), and also to analyze the release mechanism of the extract from different membrane formulations.
32409038	7	52	theme	mass	1352:1355	arg1	loss					1357:1360	a maximum mass loss	1342:1360	a maximum mass loss of 18%	1342:1367	All formulations presented high stability, reaching a maximum mass loss of 18% after seven days.
32409038	5	53	theme	biological	800:809	arg1	characteristics					811:825	the physicochemical and biological characteristics	776:825	the physicochemical and biological characteristics of flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica)	776:944	The purpose of this study was to evaluate the physicochemical and biological characteristics of flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica), and also to analyze the release mechanism of the extract from different membrane formulations.
32409038	6	54	theme	P	1081:1081	arg1	inclusion					1068:1076	the inclusion	1064:1076	the inclusion of P in the formulation	1064:1100	The results show that the inclusion of P in the formulation allows obtaining porous matrices, promotes greater homogeneity of the mixture of the silicone gel with the suspension of polysaccharides, and increases the swelling of the polymer matrix.
32409038	3	55	theme	surfactants	491:501	arg1	addition					479:486	the addition	475:486	the addition of surfactants such as Kolliphor® P188 (P) and silicone-based compounds as Silpuran® 2130 A/B (S)	475:584	Porosity and flexibility can be achieved, respectively, by the addition of surfactants such as Kolliphor® P188 (P) and silicone-based compounds as Silpuran® 2130 A/B (S).
32409038	5	56	theme	extract	996:1002	arg1	mechanism					979:987	the release mechanism	967:987	the release mechanism of the extract from different membrane formulations	967:1039	The purpose of this study was to evaluate the physicochemical and biological characteristics of flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica), and also to analyze the release mechanism of the extract from different membrane formulations.
32409038	7	57	theme	high	1317:1320	arg1	stability					1322:1330	high stability	1317:1330	high stability	1317:1330	All formulations presented high stability, reaching a maximum mass loss of 18% after seven days.
32409038	0	58	theme	skin	143:146	arg1	lesions					148:154	skin lesions	143:154	skin lesions	143:154	Flexible, dense and porous chitosan and alginate membranes containing the standardized extract of Arrabidaea chica Verlot for the treatment of skin lesions.
32409038	5	59	theme	different	1009:1017	arg1	formulations					1028:1039	different membrane formulations	1009:1039	different membrane formulations	1009:1039	The purpose of this study was to evaluate the physicochemical and biological characteristics of flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica), and also to analyze the release mechanism of the extract from different membrane formulations.
32409038	5	60	theme	porous	849:854	arg1	membranes					859:867	flexible dense and porous CA membranes	830:867	flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica)	830:944	The purpose of this study was to evaluate the physicochemical and biological characteristics of flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica), and also to analyze the release mechanism of the extract from different membrane formulations.
32409038	6	61	theme	greater	1145:1151	arg1	homogeneity					1153:1163	greater homogeneity	1145:1163	greater homogeneity of the mixture of the silicone gel with the suspension of polysaccharides	1145:1237	The results show that the inclusion of P in the formulation allows obtaining porous matrices, promotes greater homogeneity of the mixture of the silicone gel with the suspension of polysaccharides, and increases the swelling of the polymer matrix.
32409038	6	62	theme	polysaccharides	1223:1237	arg1	suspension					1209:1218	the suspension	1205:1218	the suspension of polysaccharides	1205:1237	The results show that the inclusion of P in the formulation allows obtaining porous matrices, promotes greater homogeneity of the mixture of the silicone gel with the suspension of polysaccharides, and increases the swelling of the polymer matrix.
32409038	5	63	theme	release	971:977	arg1	mechanism					979:987	the release mechanism	967:987	the release mechanism of the extract from different membrane formulations	967:1039	The purpose of this study was to evaluate the physicochemical and biological characteristics of flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica), and also to analyze the release mechanism of the extract from different membrane formulations.
32409038	5	64	theme	membranes	859:867	arg1	characteristics					811:825	the physicochemical and biological characteristics	776:825	the physicochemical and biological characteristics of flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica)	776:944	The purpose of this study was to evaluate the physicochemical and biological characteristics of flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica), and also to analyze the release mechanism of the extract from different membrane formulations.
32409038	5	65	from	mechanism	979:987	arg1	formulations					1028:1039	different membrane formulations	1009:1039	different membrane formulations	1009:1039	The purpose of this study was to evaluate the physicochemical and biological characteristics of flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica), and also to analyze the release mechanism of the extract from different membrane formulations.
32409038	10	66	theme	membrane	1707:1714	arg1	formulation					1716:1726	the membrane formulation	1703:1726	the membrane formulation	1703:1726	Incorporation efficiencies of the bioactive compound above 87% were achieved, and the addition of P and S to the membrane formulation changed the release of the A. chica extract kinetics.
32409038	6	67	theme	porous	1119:1124	arg1	matrices					1126:1133	obtaining porous matrices	1109:1133	obtaining porous matrices	1109:1133	The results show that the inclusion of P in the formulation allows obtaining porous matrices, promotes greater homogeneity of the mixture of the silicone gel with the suspension of polysaccharides, and increases the swelling of the polymer matrix.
32409038	0	68	theme	standardized	74:85	arg1	extract					87:93	the standardized extract	70:93	the standardized extract of Arrabidaea chica Verlot for the treatment of skin lesions	70:154	Flexible, dense and porous chitosan and alginate membranes containing the standardized extract of Arrabidaea chica Verlot for the treatment of skin lesions.
32409038	5	69	theme	dense	839:843	arg1	membranes					859:867	flexible dense and porous CA membranes	830:867	flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica)	830:944	The purpose of this study was to evaluate the physicochemical and biological characteristics of flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica), and also to analyze the release mechanism of the extract from different membrane formulations.
32409038	6	70	theme	polymer	1274:1280	arg1	matrix					1282:1287	the polymer matrix	1270:1287	the polymer matrix	1270:1287	The results show that the inclusion of P in the formulation allows obtaining porous matrices, promotes greater homogeneity of the mixture of the silicone gel with the suspension of polysaccharides, and increases the swelling of the polymer matrix.
32409038	5	71	theme	Verlot	928:933	arg1	extract					900:906	the standardized extract	883:906	the standardized extract of Arrabidaea chica Verlot (A. chica)	883:944	The purpose of this study was to evaluate the physicochemical and biological characteristics of flexible dense and porous CA membranes incorporating the standardized extract of Arrabidaea chica Verlot (A. chica), and also to analyze the release mechanism of the extract from different membrane formulations.
32409038	2	72	theme	dense	323:327	arg1	structure					348:356	a dense or porous flexible structure	321:356	a dense or porous flexible structure	321:356	CA films can show a dense or porous flexible structure, with characteristics tunable for different applications.
32409038	2	73	theme	tunable	380:386	arg1	characteristics					364:378	characteristics	364:378	characteristics tunable for different applications	364:413	CA films can show a dense or porous flexible structure, with characteristics tunable for different applications.
32409038	8	74	theme	flexibility	1451:1461	arg1	terms					1442:1446	terms	1442:1446	terms of flexibility and strain	1442:1472	The formulations with S showed the best performance in terms of flexibility and strain at break.
32409038	8	75	theme	strain	1467:1472	arg1	terms					1442:1446	terms	1442:1446	terms of flexibility and strain	1442:1472	The formulations with S showed the best performance in terms of flexibility and strain at break.
32409038	3	76	theme	A/B	578:580	arg1	S					583:583	Silpuran® 2130 A/B (S)	563:584	Silpuran® 2130 A/B (S)	563:584	Porosity and flexibility can be achieved, respectively, by the addition of surfactants such as Kolliphor® P188 (P) and silicone-based compounds as Silpuran® 2130 A/B (S).
32409038	0	77	theme	chitosan	27:34	arg1	membranes					49:57	Flexible, dense and porous chitosan and alginate membranes	0:57	Flexible, dense and porous chitosan and alginate membranes	0:57	Flexible, dense and porous chitosan and alginate membranes containing the standardized extract of Arrabidaea chica Verlot for the treatment of skin lesions.
32409038	1	78	theme	dressings	247:255	arg1	production					233:242	the production	229:242	the production of dressings due to the positive results on wound healing	229:300	The combination of chitosan (C) with alginate (A) has been explored for the production of dressings due to the positive results on wound healing.
32409038	3	79	theme	Silpuran®	563:571	arg1	S					583:583	Silpuran® 2130 A/B (S)	563:584	Silpuran® 2130 A/B (S)	563:584	Porosity and flexibility can be achieved, respectively, by the addition of surfactants such as Kolliphor® P188 (P) and silicone-based compounds as Silpuran® 2130 A/B (S).
32409038	2	80	theme	porous	332:337	arg1	structure					348:356	a dense or porous flexible structure	321:356	a dense or porous flexible structure	321:356	CA films can show a dense or porous flexible structure, with characteristics tunable for different applications.
32409038	0	81	theme	alginate	40:47	arg1	membranes					49:57	Flexible, dense and porous chitosan and alginate membranes	0:57	Flexible, dense and porous chitosan and alginate membranes	0:57	Flexible, dense and porous chitosan and alginate membranes containing the standardized extract of Arrabidaea chica Verlot for the treatment of skin lesions.
32409038	9	82	theme	A.	1500:1501	arg1	extract					1522:1528	A. chica standardized extract	1500:1528	A. chica standardized extract	1500:1528	The presence of A. chica standardized extract did not affect negatively the characteristics of the membranes.
32409038	2	83	theme	different	392:400	arg1	applications					402:413	different applications	392:413	different applications	392:413	CA films can show a dense or porous flexible structure, with characteristics tunable for different applications.
32409038	10	84	theme	kinetics	1772:1779	arg1	release					1740:1746	the release	1736:1746	the release of the A. chica extract kinetics	1736:1779	Incorporation efficiencies of the bioactive compound above 87% were achieved, and the addition of P and S to the membrane formulation changed the release of the A. chica extract kinetics.
32409038	1	85	theme	positive	268:275	arg1	results					277:283	the positive results	264:283	the positive results on wound healing	264:300	The combination of chitosan (C) with alginate (A) has been explored for the production of dressings due to the positive results on wound healing.
32409038	9	86	theme	standardized	1509:1520	arg1	extract					1522:1528	A. chica standardized extract	1500:1528	A. chica standardized extract	1500:1528	The presence of A. chica standardized extract did not affect negatively the characteristics of the membranes.
32409038	11	87	theme	Vero	1851:1854	arg1	cells					1856:1860	Vero cells	1851:1860	Vero cells proliferation	1851:1874	In addition, the developed formulations did not significantly affect Vero cells proliferation.
32409038	4	88	theme	skin	720:723	arg1	lesions					725:731	skin lesions	720:731	skin lesions	720:731	Furthermore, composite matrices of these polysaccharides have potential applications as devices for releasing bioactive compounds to skin lesions.
32409038	10	89	theme	Incorporation	1594:1606	arg1	efficiencies					1608:1619	Incorporation efficiencies	1594:1619	Incorporation efficiencies of the bioactive compound above 87%	1594:1655	Incorporation efficiencies of the bioactive compound above 87% were achieved, and the addition of P and S to the membrane formulation changed the release of the A. chica extract kinetics.
32399726	7	0	theme	hydrophilic	1251:1261	arg1	pGC					1279:1281	pGC	1279:1281	pGC	1279:1281	Graphical abstract Schematic representation of fabrication for porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS).
32399726	7	0	theme	hydrophilic	1251:1261	arg1	beads					1272:1276	porous hydrophilic affinity beads	1244:1276	porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS)	1244:1369	Graphical abstract Schematic representation of fabrication for porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS).
32399726	1	1	gly	glycopeptides	356:368	arg2	glycopeptides					356:368	glycopeptides	356:368	glycopeptides	356:368	A porous hydrophilic affinity bead consisting of graphene oxide and chitosan (pGC) with the honeycomb-biomimetic microchannels has been synthesized and applied as hydrophilic adsorbent for selective capture of glycopeptides.
32399726	4	2	theme	molar	813:817	arg1	ratio					819:823	molar ratio	813:823	molar ratio of human IgG to BSA tryptic digests of 1:200	813:868	The pGC beads exhibit high sensitivity (detection limit, 5 fmol), binding capacity (111.1 mg/g), enrichment selectivity (molar ratio of human IgG to BSA tryptic digests of 1:200), and recovery yield (89.78%).
32399726	8	3	gly	glycopeptides	1447:1459	arg2	glycopeptides					1447:1459	capturing and identifying low-abundant glycopeptides	1408:1459	capturing and identifying low-abundant glycopeptides from biological samples	1408:1483	The pGC was successfully applied to capturing and identifying low-abundant glycopeptides from biological samples.
32399726	8	3	gly	glycopeptides	1447:1459	arg1	samples					1477:1483	biological samples	1466:1483	biological samples	1466:1483	The pGC was successfully applied to capturing and identifying low-abundant glycopeptides from biological samples.
32399726	7	4	theme	affinity	1263:1270	arg1	pGC					1279:1281	pGC	1279:1281	pGC	1279:1281	Graphical abstract Schematic representation of fabrication for porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS).
32399726	7	4	theme	affinity	1263:1270	arg1	beads					1272:1276	porous hydrophilic affinity beads	1244:1276	porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS)	1244:1369	Graphical abstract Schematic representation of fabrication for porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS).
32399726	4	5	theme	recovery	876:883	arg1	yield					885:889	recovery yield	876:889	recovery yield (89.78%)	876:898	The pGC beads exhibit high sensitivity (detection limit, 5 fmol), binding capacity (111.1 mg/g), enrichment selectivity (molar ratio of human IgG to BSA tryptic digests of 1:200), and recovery yield (89.78%).
32399726	4	5	theme	recovery	876:883	arg1	%					897:897	89.78%	892:897	89.78%	892:897	The pGC beads exhibit high sensitivity (detection limit, 5 fmol), binding capacity (111.1 mg/g), enrichment selectivity (molar ratio of human IgG to BSA tryptic digests of 1:200), and recovery yield (89.78%).
32399726	3	6	from	glycopeptides	568:580	arg1	chitosan					622:629	chitosan	622:629	chitosan	622:629	Based on the multivalent hydrophilic interactions between glycan moieties on glycopeptides and amino groups and hydroxyl groups on chitosan, the glycopeptides were enriched and separated by pGC beads.
32399726	2	7	theme	open-porous	390:400	arg1	structure					402:410	open-porous structure	390:410	open-porous structure	390:410	The pGC beads have open-porous structure, honeycomb-like microchannels, large interior voids, and hydrophilic property.
32399726	3	8	from	groups	612:617	arg1	chitosan					622:629	chitosan	622:629	chitosan	622:629	Based on the multivalent hydrophilic interactions between glycan moieties on glycopeptides and amino groups and hydroxyl groups on chitosan, the glycopeptides were enriched and separated by pGC beads.
32399726	2	9	theme	pGC	375:377	arg1	beads					379:383	The pGC beads	371:383	The pGC beads	371:383	The pGC beads have open-porous structure, honeycomb-like microchannels, large interior voids, and hydrophilic property.
32399726	2	10	contain	have	385:388	arg1	beads					379:383	The pGC beads	371:383	The pGC beads	371:383	The pGC beads have open-porous structure, honeycomb-like microchannels, large interior voids, and hydrophilic property.
32399726	2	10	contain	have	385:388	arg2	property					481:488	hydrophilic property	469:488	hydrophilic property	469:488	The pGC beads have open-porous structure, honeycomb-like microchannels, large interior voids, and hydrophilic property.
32399726	2	10	contain	have	385:388	arg2	structure					402:410	open-porous structure	390:410	open-porous structure	390:410	The pGC beads have open-porous structure, honeycomb-like microchannels, large interior voids, and hydrophilic property.
32399726	2	10	contain	have	385:388	arg2	voids					458:462	large interior voids	443:462	large interior voids	443:462	The pGC beads have open-porous structure, honeycomb-like microchannels, large interior voids, and hydrophilic property.
32399726	2	10	contain	have	385:388	arg2	microchannels					428:440	honeycomb-like microchannels	413:440	honeycomb-like microchannels	413:440	The pGC beads have open-porous structure, honeycomb-like microchannels, large interior voids, and hydrophilic property.
32399726	3	11	theme	amino	586:590	arg1	groups					592:597	amino groups	586:597	amino groups	586:597	Based on the multivalent hydrophilic interactions between glycan moieties on glycopeptides and amino groups and hydroxyl groups on chitosan, the glycopeptides were enriched and separated by pGC beads.
32399726	8	12	theme	biological	1466:1475	arg1	samples					1477:1483	biological samples	1466:1483	biological samples	1466:1483	The pGC was successfully applied to capturing and identifying low-abundant glycopeptides from biological samples.
32399726	0	13	theme	selective	110:118	arg1	capture					120:126	the selective capture	106:126	the selective capture of glycopeptides	106:143	Porous graphene oxide/chitosan beads with honeycomb-biomimetic microchannels as hydrophilic adsorbent for the selective capture of glycopeptides.
32399726	6	14	from	application	1126:1136	arg1	research					1171:1178	glycoproteomics research	1155:1178	glycoproteomics research	1155:1178	These experimental results demonstrate the practical application of the method in glycoproteomics research.
32399726	3	15	theme	glycan	549:554	arg1	moieties					556:563	glycan moieties	549:563	glycan moieties on glycopeptides and amino groups and hydroxyl groups on chitosan	549:629	Based on the multivalent hydrophilic interactions between glycan moieties on glycopeptides and amino groups and hydroxyl groups on chitosan, the glycopeptides were enriched and separated by pGC beads.
32399726	0	16	theme	glycopeptides	131:143	arg1	capture					120:126	the selective capture	106:126	the selective capture of glycopeptides	106:143	Porous graphene oxide/chitosan beads with honeycomb-biomimetic microchannels as hydrophilic adsorbent for the selective capture of glycopeptides.
32399726	4	17	theme	binding	758:764	arg1	111.1 mg/g					776:785	111.1 mg/g	776:785	111.1 mg/g	776:785	The pGC beads exhibit high sensitivity (detection limit, 5 fmol), binding capacity (111.1 mg/g), enrichment selectivity (molar ratio of human IgG to BSA tryptic digests of 1:200), and recovery yield (89.78%).
32399726	4	17	theme	binding	758:764	arg1	capacity					766:773	binding capacity	758:773	binding capacity (111.1 mg/g)	758:786	The pGC beads exhibit high sensitivity (detection limit, 5 fmol), binding capacity (111.1 mg/g), enrichment selectivity (molar ratio of human IgG to BSA tryptic digests of 1:200), and recovery yield (89.78%).
32399726	3	18	from	groups	592:597	arg1	chitosan					622:629	chitosan	622:629	chitosan	622:629	Based on the multivalent hydrophilic interactions between glycan moieties on glycopeptides and amino groups and hydroxyl groups on chitosan, the glycopeptides were enriched and separated by pGC beads.
32399726	1	19	theme	graphene	195:202	arg1	pGC					224:226	pGC	224:226	pGC	224:226	A porous hydrophilic affinity bead consisting of graphene oxide and chitosan (pGC) with the honeycomb-biomimetic microchannels has been synthesized and applied as hydrophilic adsorbent for selective capture of glycopeptides.
32399726	1	19	theme	graphene	195:202	arg1	oxide					204:208	graphene oxide	195:208	graphene oxide	195:208	A porous hydrophilic affinity bead consisting of graphene oxide and chitosan (pGC) with the honeycomb-biomimetic microchannels has been synthesized and applied as hydrophilic adsorbent for selective capture of glycopeptides.
32399726	7	20	theme	fabrication	1228:1238	arg1	representation					1210:1223	Graphical abstract Schematic representation	1181:1223	Graphical abstract Schematic representation of fabrication for porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS).	1181:1370	Graphical abstract Schematic representation of fabrication for porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS).
32399726	4	21	theme	1:200	864:868	arg1	digests					853:859	BSA tryptic digests	841:859	BSA tryptic digests of 1:200	841:868	The pGC beads exhibit high sensitivity (detection limit, 5 fmol), binding capacity (111.1 mg/g), enrichment selectivity (molar ratio of human IgG to BSA tryptic digests of 1:200), and recovery yield (89.78%).
32399726	4	22	theme	pGC	696:698	arg1	beads					700:704	The pGC beads	692:704	The pGC beads	692:704	The pGC beads exhibit high sensitivity (detection limit, 5 fmol), binding capacity (111.1 mg/g), enrichment selectivity (molar ratio of human IgG to BSA tryptic digests of 1:200), and recovery yield (89.78%).
32399726	3	23	gly	glycopeptides	568:580	arg2	glycopeptides					568:580	glycopeptides	568:580	glycopeptides	568:580	Based on the multivalent hydrophilic interactions between glycan moieties on glycopeptides and amino groups and hydroxyl groups on chitosan, the glycopeptides were enriched and separated by pGC beads.
32399726	2	24	theme	large	443:447	arg1	voids					458:462	large interior voids	443:462	large interior voids	443:462	The pGC beads have open-porous structure, honeycomb-like microchannels, large interior voids, and hydrophilic property.
32399726	5	25	gly	N-glycosylated	1010:1023	arg1	proteins					1025:1032	152 N-glycosylated proteins	1006:1032	152 N-glycosylated proteins	1006:1032	By combing pGC beads and nano LC-MS/MS analysis, a total of 325 N-glycosylated peptides corresponding to 152 N-glycosylated proteins were identified from 2 μL human serum.
32399726	6	26	theme	experimental	1079:1090	arg1	results					1092:1098	These experimental results	1073:1098	These experimental results	1073:1098	These experimental results demonstrate the practical application of the method in glycoproteomics research.
32399726	7	27	theme	honeycomb-biomimetic	1289:1308	arg1	microchannels					1310:1322	honeycomb-biomimetic microchannels	1289:1322	honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS)	1289:1369	Graphical abstract Schematic representation of fabrication for porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS).
32399726	5	28	theme	nano	926:929	arg1	analysis					940:947	nano LC-MS/MS analysis	926:947	nano LC-MS/MS analysis	926:947	By combing pGC beads and nano LC-MS/MS analysis, a total of 325 N-glycosylated peptides corresponding to 152 N-glycosylated proteins were identified from 2 μL human serum.
32399726	4	29	theme	detection	732:740	arg1	5 fmol					749:754	5 fmol	749:754	5 fmol	749:754	The pGC beads exhibit high sensitivity (detection limit, 5 fmol), binding capacity (111.1 mg/g), enrichment selectivity (molar ratio of human IgG to BSA tryptic digests of 1:200), and recovery yield (89.78%).
32399726	4	29	theme	detection	732:740	arg1	limit					742:746	detection limit	732:746	detection limit	732:746	The pGC beads exhibit high sensitivity (detection limit, 5 fmol), binding capacity (111.1 mg/g), enrichment selectivity (molar ratio of human IgG to BSA tryptic digests of 1:200), and recovery yield (89.78%).
32399726	0	30	theme	Porous	0:5	arg1	graphene					7:14	Porous graphene	0:14	Porous graphene	0:14	Porous graphene oxide/chitosan beads with honeycomb-biomimetic microchannels as hydrophilic adsorbent for the selective capture of glycopeptides.
32399726	6	31	theme	method	1145:1150	arg1	application					1126:1136	the practical application	1112:1136	the practical application of the method in glycoproteomics research	1112:1178	These experimental results demonstrate the practical application of the method in glycoproteomics research.
32399726	3	32	from	moieties	556:563	arg1	glycopeptides					568:580	glycopeptides	568:580	glycopeptides	568:580	Based on the multivalent hydrophilic interactions between glycan moieties on glycopeptides and amino groups and hydroxyl groups on chitosan, the glycopeptides were enriched and separated by pGC beads.
32399726	3	32	from	moieties	556:563	arg1	groups					592:597	amino groups	586:597	amino groups	586:597	Based on the multivalent hydrophilic interactions between glycan moieties on glycopeptides and amino groups and hydroxyl groups on chitosan, the glycopeptides were enriched and separated by pGC beads.
32399726	5	33	theme	LC-MS/MS	931:938	arg1	analysis					940:947	nano LC-MS/MS analysis	926:947	nano LC-MS/MS analysis	926:947	By combing pGC beads and nano LC-MS/MS analysis, a total of 325 N-glycosylated peptides corresponding to 152 N-glycosylated proteins were identified from 2 μL human serum.
32399726	8	34	from	samples	1477:1483	arg1	glycopeptides					1447:1459	capturing and identifying low-abundant glycopeptides	1408:1459	capturing and identifying low-abundant glycopeptides from biological samples	1408:1483	The pGC was successfully applied to capturing and identifying low-abundant glycopeptides from biological samples.
32399726	7	35	theme	Graphical	1181:1189	arg1	representation					1210:1223	Graphical abstract Schematic representation	1181:1223	Graphical abstract Schematic representation of fabrication for porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS).	1181:1370	Graphical abstract Schematic representation of fabrication for porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS).
32399726	7	36	with	beads	1272:1276	arg1	microchannels					1310:1322	honeycomb-biomimetic microchannels	1289:1322	honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS)	1289:1369	Graphical abstract Schematic representation of fabrication for porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS).
32399726	4	37	theme	enrichment	789:798	arg1	selectivity					800:810	enrichment selectivity	789:810	enrichment selectivity (molar ratio of human IgG to BSA tryptic digests of 1:200)	789:869	The pGC beads exhibit high sensitivity (detection limit, 5 fmol), binding capacity (111.1 mg/g), enrichment selectivity (molar ratio of human IgG to BSA tryptic digests of 1:200), and recovery yield (89.78%).
32399726	2	38	theme	hydrophilic	469:479	arg1	property					481:488	hydrophilic property	469:488	hydrophilic property	469:488	The pGC beads have open-porous structure, honeycomb-like microchannels, large interior voids, and hydrophilic property.
32399726	7	39	theme	abstract	1191:1198	arg1	representation					1210:1223	Graphical abstract Schematic representation	1181:1223	Graphical abstract Schematic representation of fabrication for porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS).	1181:1370	Graphical abstract Schematic representation of fabrication for porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS).
32399726	8	40	theme	capturing	1408:1416	arg1	glycopeptides					1447:1459	capturing and identifying low-abundant glycopeptides	1408:1459	capturing and identifying low-abundant glycopeptides from biological samples	1408:1483	The pGC was successfully applied to capturing and identifying low-abundant glycopeptides from biological samples.
32399726	0	41	gly	glycopeptides	131:143	arg2	glycopeptides					131:143	glycopeptides	131:143	glycopeptides	131:143	Porous graphene oxide/chitosan beads with honeycomb-biomimetic microchannels as hydrophilic adsorbent for the selective capture of glycopeptides.
32399726	1	42	theme	affinity	167:174	arg1	bead					176:179	A porous hydrophilic affinity bead	146:179	A porous hydrophilic affinity bead consisting of graphene oxide and chitosan (pGC) with the honeycomb-biomimetic microchannels	146:271	A porous hydrophilic affinity bead consisting of graphene oxide and chitosan (pGC) with the honeycomb-biomimetic microchannels has been synthesized and applied as hydrophilic adsorbent for selective capture of glycopeptides.
32399726	0	43	theme	honeycomb-biomimetic	42:61	arg1	microchannels					63:75	honeycomb-biomimetic microchannels	42:75	honeycomb-biomimetic microchannels	42:75	Porous graphene oxide/chitosan beads with honeycomb-biomimetic microchannels as hydrophilic adsorbent for the selective capture of glycopeptides.
32399726	1	44	theme	selective	335:343	arg1	capture					345:351	selective capture	335:351	selective capture of glycopeptides	335:368	A porous hydrophilic affinity bead consisting of graphene oxide and chitosan (pGC) with the honeycomb-biomimetic microchannels has been synthesized and applied as hydrophilic adsorbent for selective capture of glycopeptides.
32399726	7	45	dep	oxide	1342:1346	arg1	GO					1349:1350	GO	1349:1350	GO	1349:1350	Graphical abstract Schematic representation of fabrication for porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS).
32399726	3	46	theme	hydroxyl	603:610	arg1	groups					612:617	hydroxyl groups	603:617	hydroxyl groups	603:617	Based on the multivalent hydrophilic interactions between glycan moieties on glycopeptides and amino groups and hydroxyl groups on chitosan, the glycopeptides were enriched and separated by pGC beads.
32399726	4	47	theme	tryptic	845:851	arg1	digests					853:859	BSA tryptic digests	841:859	BSA tryptic digests of 1:200	841:868	The pGC beads exhibit high sensitivity (detection limit, 5 fmol), binding capacity (111.1 mg/g), enrichment selectivity (molar ratio of human IgG to BSA tryptic digests of 1:200), and recovery yield (89.78%).
32399726	2	48	theme	interior	449:456	arg1	voids					458:462	large interior voids	443:462	large interior voids	443:462	The pGC beads have open-porous structure, honeycomb-like microchannels, large interior voids, and hydrophilic property.
32399726	5	49	gly	N-glycosylated	965:978	arg1	peptides					980:987	325 N-glycosylated peptides	961:987	325 N-glycosylated peptides corresponding to 152 N-glycosylated proteins	961:1032	By combing pGC beads and nano LC-MS/MS analysis, a total of 325 N-glycosylated peptides corresponding to 152 N-glycosylated proteins were identified from 2 μL human serum.
32399726	4	50	theme	BSA	841:843	arg1	digests					853:859	BSA tryptic digests	841:859	BSA tryptic digests of 1:200	841:868	The pGC beads exhibit high sensitivity (detection limit, 5 fmol), binding capacity (111.1 mg/g), enrichment selectivity (molar ratio of human IgG to BSA tryptic digests of 1:200), and recovery yield (89.78%).
32399726	0	51	with	beads	31:35	arg1	microchannels					63:75	honeycomb-biomimetic microchannels	42:75	honeycomb-biomimetic microchannels	42:75	Porous graphene oxide/chitosan beads with honeycomb-biomimetic microchannels as hydrophilic adsorbent for the selective capture of glycopeptides.
32399726	3	52	theme	multivalent	504:514	arg1	interactions					528:539	the multivalent hydrophilic interactions	500:539	the multivalent hydrophilic interactions between glycan moieties on glycopeptides and amino groups and hydroxyl groups on chitosan	500:629	Based on the multivalent hydrophilic interactions between glycan moieties on glycopeptides and amino groups and hydroxyl groups on chitosan, the glycopeptides were enriched and separated by pGC beads.
32399726	8	53	theme	low-abundant	1434:1445	arg1	glycopeptides					1447:1459	capturing and identifying low-abundant glycopeptides	1408:1459	capturing and identifying low-abundant glycopeptides from biological samples	1408:1483	The pGC was successfully applied to capturing and identifying low-abundant glycopeptides from biological samples.
32399726	6	54	theme	practical	1116:1124	arg1	application					1126:1136	the practical application	1112:1136	the practical application of the method in glycoproteomics research	1112:1178	These experimental results demonstrate the practical application of the method in glycoproteomics research.
32399726	3	55	gly	glycopeptides	636:648	arg2	glycopeptides					636:648	the glycopeptides	632:648	the glycopeptides	632:648	Based on the multivalent hydrophilic interactions between glycan moieties on glycopeptides and amino groups and hydroxyl groups on chitosan, the glycopeptides were enriched and separated by pGC beads.
32399726	1	56	theme	glycopeptides	356:368	arg1	capture					345:351	selective capture	335:351	selective capture of glycopeptides	335:368	A porous hydrophilic affinity bead consisting of graphene oxide and chitosan (pGC) with the honeycomb-biomimetic microchannels has been synthesized and applied as hydrophilic adsorbent for selective capture of glycopeptides.
32399726	1	57	with	chitosan	214:221	arg1	microchannels					259:271	the honeycomb-biomimetic microchannels	234:271	the honeycomb-biomimetic microchannels	234:271	A porous hydrophilic affinity bead consisting of graphene oxide and chitosan (pGC) with the honeycomb-biomimetic microchannels has been synthesized and applied as hydrophilic adsorbent for selective capture of glycopeptides.
32399726	8	58	theme	identifying	1422:1432	arg1	glycopeptides					1447:1459	capturing and identifying low-abundant glycopeptides	1408:1459	capturing and identifying low-abundant glycopeptides from biological samples	1408:1483	The pGC was successfully applied to capturing and identifying low-abundant glycopeptides from biological samples.
32399726	5	59	theme	pGC	912:914	arg1	beads					916:920	combing pGC beads	904:920	combing pGC beads	904:920	By combing pGC beads and nano LC-MS/MS analysis, a total of 325 N-glycosylated peptides corresponding to 152 N-glycosylated proteins were identified from 2 μL human serum.
32399726	4	60	theme	IgG	834:836	arg1	ratio					819:823	molar ratio	813:823	molar ratio of human IgG to BSA tryptic digests of 1:200	813:868	The pGC beads exhibit high sensitivity (detection limit, 5 fmol), binding capacity (111.1 mg/g), enrichment selectivity (molar ratio of human IgG to BSA tryptic digests of 1:200), and recovery yield (89.78%).
32399726	4	61	dep	selectivity	800:810	arg1	ratio					819:823	molar ratio	813:823	molar ratio of human IgG to BSA tryptic digests of 1:200	813:868	The pGC beads exhibit high sensitivity (detection limit, 5 fmol), binding capacity (111.1 mg/g), enrichment selectivity (molar ratio of human IgG to BSA tryptic digests of 1:200), and recovery yield (89.78%).
32399726	5	62	theme	N-glycosylated	965:978	arg1	peptides					980:987	325 N-glycosylated peptides	961:987	325 N-glycosylated peptides corresponding to 152 N-glycosylated proteins	961:1032	By combing pGC beads and nano LC-MS/MS analysis, a total of 325 N-glycosylated peptides corresponding to 152 N-glycosylated proteins were identified from 2 μL human serum.
32399726	4	63	theme	human	828:832	arg1	IgG					834:836	human IgG	828:836	human IgG	828:836	The pGC beads exhibit high sensitivity (detection limit, 5 fmol), binding capacity (111.1 mg/g), enrichment selectivity (molar ratio of human IgG to BSA tryptic digests of 1:200), and recovery yield (89.78%).
32399726	5	64	theme	N-glycosylated	1010:1023	arg1	proteins					1025:1032	152 N-glycosylated proteins	1006:1032	152 N-glycosylated proteins	1006:1032	By combing pGC beads and nano LC-MS/MS analysis, a total of 325 N-glycosylated peptides corresponding to 152 N-glycosylated proteins were identified from 2 μL human serum.
32399726	1	65	theme	porous	148:153	arg1	bead					176:179	A porous hydrophilic affinity bead	146:179	A porous hydrophilic affinity bead consisting of graphene oxide and chitosan (pGC) with the honeycomb-biomimetic microchannels	146:271	A porous hydrophilic affinity bead consisting of graphene oxide and chitosan (pGC) with the honeycomb-biomimetic microchannels has been synthesized and applied as hydrophilic adsorbent for selective capture of glycopeptides.
32399726	6	66	theme	glycoproteomics	1155:1169	arg1	research					1171:1178	glycoproteomics research	1155:1178	glycoproteomics research	1155:1178	These experimental results demonstrate the practical application of the method in glycoproteomics research.
32399726	1	67	with	oxide	204:208	arg1	microchannels					259:271	the honeycomb-biomimetic microchannels	234:271	the honeycomb-biomimetic microchannels	234:271	A porous hydrophilic affinity bead consisting of graphene oxide and chitosan (pGC) with the honeycomb-biomimetic microchannels has been synthesized and applied as hydrophilic adsorbent for selective capture of glycopeptides.
32399726	5	68	theme	combing	904:910	arg1	beads					916:920	combing pGC beads	904:920	combing pGC beads	904:920	By combing pGC beads and nano LC-MS/MS analysis, a total of 325 N-glycosylated peptides corresponding to 152 N-glycosylated proteins were identified from 2 μL human serum.
32399726	5	69	theme	peptides	980:987	arg1	total					952:956	a total	950:956	a total of 325 N-glycosylated peptides corresponding to 152 N-glycosylated proteins	950:1032	By combing pGC beads and nano LC-MS/MS analysis, a total of 325 N-glycosylated peptides corresponding to 152 N-glycosylated proteins were identified from 2 μL human serum.
32399726	1	70	theme	hydrophilic	155:165	arg1	bead					176:179	A porous hydrophilic affinity bead	146:179	A porous hydrophilic affinity bead consisting of graphene oxide and chitosan (pGC) with the honeycomb-biomimetic microchannels	146:271	A porous hydrophilic affinity bead consisting of graphene oxide and chitosan (pGC) with the honeycomb-biomimetic microchannels has been synthesized and applied as hydrophilic adsorbent for selective capture of glycopeptides.
32399726	5	71	theme	2 μL	1055:1058	arg1	serum					1066:1070	2 μL human serum	1055:1070	2 μL human serum	1055:1070	By combing pGC beads and nano LC-MS/MS analysis, a total of 325 N-glycosylated peptides corresponding to 152 N-glycosylated proteins were identified from 2 μL human serum.
32399726	1	72	theme	honeycomb-biomimetic	238:257	arg1	microchannels					259:271	the honeycomb-biomimetic microchannels	234:271	the honeycomb-biomimetic microchannels	234:271	A porous hydrophilic affinity bead consisting of graphene oxide and chitosan (pGC) with the honeycomb-biomimetic microchannels has been synthesized and applied as hydrophilic adsorbent for selective capture of glycopeptides.
32399726	5	73	theme	human	1060:1064	arg1	serum					1066:1070	2 μL human serum	1055:1070	2 μL human serum	1055:1070	By combing pGC beads and nano LC-MS/MS analysis, a total of 325 N-glycosylated peptides corresponding to 152 N-glycosylated proteins were identified from 2 μL human serum.
32399726	4	74	dep	sensitivity	719:729	arg1	5 fmol					749:754	5 fmol	749:754	5 fmol	749:754	The pGC beads exhibit high sensitivity (detection limit, 5 fmol), binding capacity (111.1 mg/g), enrichment selectivity (molar ratio of human IgG to BSA tryptic digests of 1:200), and recovery yield (89.78%).
32399726	4	74	dep	sensitivity	719:729	arg1	limit					742:746	detection limit	732:746	detection limit	732:746	The pGC beads exhibit high sensitivity (detection limit, 5 fmol), binding capacity (111.1 mg/g), enrichment selectivity (molar ratio of human IgG to BSA tryptic digests of 1:200), and recovery yield (89.78%).
32399726	3	75	theme	hydrophilic	516:526	arg1	interactions					528:539	the multivalent hydrophilic interactions	500:539	the multivalent hydrophilic interactions between glycan moieties on glycopeptides and amino groups and hydroxyl groups on chitosan	500:629	Based on the multivalent hydrophilic interactions between glycan moieties on glycopeptides and amino groups and hydroxyl groups on chitosan, the glycopeptides were enriched and separated by pGC beads.
32399726	2	76	theme	honeycomb-like	413:426	arg1	microchannels					428:440	honeycomb-like microchannels	413:440	honeycomb-like microchannels	413:440	The pGC beads have open-porous structure, honeycomb-like microchannels, large interior voids, and hydrophilic property.
32399726	4	77	theme	high	714:717	arg1	sensitivity					719:729	high sensitivity	714:729	high sensitivity (detection limit, 5 fmol)	714:755	The pGC beads exhibit high sensitivity (detection limit, 5 fmol), binding capacity (111.1 mg/g), enrichment selectivity (molar ratio of human IgG to BSA tryptic digests of 1:200), and recovery yield (89.78%).
32399726	3	78	theme	pGC	681:683	arg1	beads					685:689	pGC beads	681:689	pGC beads	681:689	Based on the multivalent hydrophilic interactions between glycan moieties on glycopeptides and amino groups and hydroxyl groups on chitosan, the glycopeptides were enriched and separated by pGC beads.
32399726	7	79	theme	Schematic	1200:1208	arg1	representation					1210:1223	Graphical abstract Schematic representation	1181:1223	Graphical abstract Schematic representation of fabrication for porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS).	1181:1370	Graphical abstract Schematic representation of fabrication for porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS).
32399726	7	80	theme	graphene	1333:1340	arg1	oxide					1342:1346	graphene oxide	1333:1346	graphene oxide (GO)	1333:1351	Graphical abstract Schematic representation of fabrication for porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS).
32399726	7	81	theme	porous	1244:1249	arg1	pGC					1279:1281	pGC	1279:1281	pGC	1279:1281	Graphical abstract Schematic representation of fabrication for porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS).
32399726	7	81	theme	porous	1244:1249	arg1	beads					1272:1276	porous hydrophilic affinity beads	1244:1276	porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS)	1244:1369	Graphical abstract Schematic representation of fabrication for porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS).
33486179	9	0	theme	content	1647:1653	arg1	processing					1615:1624	the complex processing	1603:1624	the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol	1603:1719	Sorbitol was found to be many times more impacting than glycerol in most categories, which is due to the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol.
33486179	4	1	theme	Previous	587:594	arg1	studies					596:602	Previous studies	587:602	Previous studies evaluating the Life Cycle Assessment (LCA) of starch-based composites	587:672	Previous studies evaluating the Life Cycle Assessment (LCA) of starch-based composites have focused on commercially available starches, although other non-conventional starches can also be used to produce biopolymers.
33486179	10	2	theme	Brazil	1736:1741	arg1	fibers					1747:1752	Brazil nut fibers	1736:1752	Brazil nut fibers	1736:1752	Additionally, Brazil nut fibers are presented as an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin.
33486179	10	2	theme	Brazil	1736:1741	arg1	filler					1810:1815	an eco-friendly and low-burden natural filler	1771:1815	an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin	1771:1874	Additionally, Brazil nut fibers are presented as an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin.
33486179	6	3	theme	fiber	1199:1203	arg1	composites					1205:1214	conventional PLA-Brazil nut fiber and PP-glass fiber composites	1152:1214	composites	1205:1214	Results indicated that the starch-based biocomposites were less impacting than conventional PLA-Brazil nut fiber and PP-glass fiber composites.
33486179	9	4	dep	impacting	1543:1551	arg1	more					1538:1541	more	1538:1541	more	1538:1541	Sorbitol was found to be many times more impacting than glycerol in most categories, which is due to the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol.
33486179	3	5	theme	sustainable	548:558	arg1	nature					560:565	the sustainable nature	544:565	the sustainable nature of these materials	544:584	However, the environmental burden of the many production processes biopolymers and their components undergo better reflect the sustainable nature of these materials.
33486179	9	6	theme	similar	1677:1683	arg1	properties					1696:1705	similar mechanical properties	1677:1705	similar mechanical properties than glycerol	1677:1719	Sorbitol was found to be many times more impacting than glycerol in most categories, which is due to the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol.
33486179	4	7	theme	composites	663:672	arg1	LCA					642:644	LCA	642:644	LCA	642:644	Previous studies evaluating the Life Cycle Assessment (LCA) of starch-based composites have focused on commercially available starches, although other non-conventional starches can also be used to produce biopolymers.
33486179	4	7	theme	composites	663:672	arg1	Assessment					630:639	the Life Cycle Assessment	615:639	the Life Cycle Assessment (LCA) of starch-based composites	615:672	Previous studies evaluating the Life Cycle Assessment (LCA) of starch-based composites have focused on commercially available starches, although other non-conventional starches can also be used to produce biopolymers.
33486179	6	8	theme	nut	1176:1178	arg1	fiber					1180:1184	conventional PLA-Brazil nut fiber and PP-glass fiber composites	1152:1214	fiber	1180:1184	Results indicated that the starch-based biocomposites were less impacting than conventional PLA-Brazil nut fiber and PP-glass fiber composites.
33486179	3	9	theme	materials	576:584	arg1	nature					560:565	the sustainable nature	544:565	the sustainable nature of these materials	544:584	However, the environmental burden of the many production processes biopolymers and their components undergo better reflect the sustainable nature of these materials.
33486179	1	10	theme	global	176:181	arg1	crisis					201:206	the global plastic pollution crisis	172:206	the global plastic pollution crisis	172:206	Amidst the global plastic pollution crisis, bio-based polymers have been proposed as a potential substitute to tackle this issue.
33486179	6	11	theme	conventional	1152:1163	arg1	fiber					1180:1184	conventional PLA-Brazil nut fiber and PP-glass fiber composites	1152:1214	fiber	1180:1184	Results indicated that the starch-based biocomposites were less impacting than conventional PLA-Brazil nut fiber and PP-glass fiber composites.
33486179	8	12	theme	multiple	1384:1391	arg1	irrigation					1425:1434	irrigation	1425:1434	irrigation	1425:1434	The main drivers of these differences were the multiple agricultural practices, such as irrigation and fertilization, and the crop efficiency for starch extraction.
33486179	8	12	theme	multiple	1384:1391	arg1	drivers					1346:1352	The main drivers	1337:1352	The main drivers of these differences	1337:1373	The main drivers of these differences were the multiple agricultural practices, such as irrigation and fertilization, and the crop efficiency for starch extraction.
33486179	8	12	theme	multiple	1384:1391	arg1	practices					1406:1414	the multiple agricultural practices	1380:1414	the multiple agricultural practices	1380:1414	The main drivers of these differences were the multiple agricultural practices, such as irrigation and fertilization, and the crop efficiency for starch extraction.
33486179	8	12	theme	multiple	1384:1391	arg1	fertilization					1440:1452	fertilization	1440:1452	fertilization	1440:1452	The main drivers of these differences were the multiple agricultural practices, such as irrigation and fertilization, and the crop efficiency for starch extraction.
33486179	0	13	theme	biocomposite	106:117	arg1	production					119:128	starch-Brazil nut fiber biocomposite production	82:128	starch-Brazil nut fiber biocomposite production	82:128	Influence of the source of starch and plasticizers on the environmental burden of starch-Brazil nut fiber biocomposite production: A life cycle assessment approach.
33486179	5	14	theme	present	843:849	arg1	study					851:855	the present study	839:855	the present study	839:855	To address this knowledge gap, in the present study we evaluated the LCA of starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol.
33486179	8	15	theme	crop	1463:1466	arg1	efficiency					1468:1477	the crop efficiency	1459:1477	the crop efficiency for starch extraction	1459:1499	The main drivers of these differences were the multiple agricultural practices, such as irrigation and fertilization, and the crop efficiency for starch extraction.
33486179	0	16	theme	life	133:136	arg1	approach					155:162	A life cycle assessment approach	131:162	Influence of the source of starch and plasticizers on the environmental burden of starch-Brazil nut fiber biocomposite production: A life cycle assessment approach.	0:163	Influence of the source of starch and plasticizers on the environmental burden of starch-Brazil nut fiber biocomposite production: A life cycle assessment approach.
33486179	5	17	theme	different	1026:1034	arg1	sorbitol					1063:1070	sorbitol	1063:1070	sorbitol	1063:1070	To address this knowledge gap, in the present study we evaluated the LCA of starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol.
33486179	5	17	theme	different	1026:1034	arg1	glycerol					1050:1057	glycerol	1050:1057	glycerol	1050:1057	To address this knowledge gap, in the present study we evaluated the LCA of starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol.
33486179	5	17	theme	different	1026:1034	arg1	plasticizers					1036:1047	two different plasticizers	1022:1047	two different plasticizers	1022:1047	To address this knowledge gap, in the present study we evaluated the LCA of starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol.
33486179	10	18	theme	due	1817:1819	arg1	fibers					1747:1752	Brazil nut fibers	1736:1752	Brazil nut fibers	1736:1752	Additionally, Brazil nut fibers are presented as an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin.
33486179	10	18	theme	due	1817:1819	arg1	filler					1810:1815	an eco-friendly and low-burden natural filler	1771:1815	an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin	1771:1874	Additionally, Brazil nut fibers are presented as an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin.
33486179	1	19	dep	potential	252:260	arg1	substitute					262:271	substitute	262:271	substitute to tackle this issue	262:292	Amidst the global plastic pollution crisis, bio-based polymers have been proposed as a potential substitute to tackle this issue.
33486179	4	20	theme	non-conventional	738:753	arg1	starches					755:762	other non-conventional starches	732:762	other non-conventional starches	732:762	Previous studies evaluating the Life Cycle Assessment (LCA) of starch-based composites have focused on commercially available starches, although other non-conventional starches can also be used to produce biopolymers.
33486179	0	21	theme	assessment	144:153	arg1	approach					155:162	A life cycle assessment approach	131:162	Influence of the source of starch and plasticizers on the environmental burden of starch-Brazil nut fiber biocomposite production: A life cycle assessment approach.	0:163	Influence of the source of starch and plasticizers on the environmental burden of starch-Brazil nut fiber biocomposite production: A life cycle assessment approach.
33486179	10	22	theme	agricultural	1850:1861	arg1	origin					1869:1874	agricultural waste origin	1850:1874	agricultural waste origin	1850:1874	Additionally, Brazil nut fibers are presented as an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin.
33486179	9	23	theme	many	1527:1530	arg1	times					1532:1536	many times	1527:1536	many times more impacting than glycerol in most categories, which is due to the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol	1527:1719	Sorbitol was found to be many times more impacting than glycerol in most categories, which is due to the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol.
33486179	8	24	theme	main	1341:1344	arg1	irrigation					1425:1434	irrigation	1425:1434	irrigation	1425:1434	The main drivers of these differences were the multiple agricultural practices, such as irrigation and fertilization, and the crop efficiency for starch extraction.
33486179	8	24	theme	main	1341:1344	arg1	practices					1406:1414	the multiple agricultural practices	1380:1414	the multiple agricultural practices	1380:1414	The main drivers of these differences were the multiple agricultural practices, such as irrigation and fertilization, and the crop efficiency for starch extraction.
33486179	8	24	theme	main	1341:1344	arg1	drivers					1346:1352	The main drivers	1337:1352	The main drivers of these differences	1337:1373	The main drivers of these differences were the multiple agricultural practices, such as irrigation and fertilization, and the crop efficiency for starch extraction.
33486179	8	24	theme	main	1341:1344	arg1	fertilization					1440:1452	fertilization	1440:1452	fertilization	1440:1452	The main drivers of these differences were the multiple agricultural practices, such as irrigation and fertilization, and the crop efficiency for starch extraction.
33486179	7	25	theme	plasticizer	1240:1250	arg1	type					1221:1224	The type	1217:1224	The type of starch and plasticizer	1217:1250	The type of starch and plasticizer significantly influenced the environmental load of the production of the composites.
33486179	5	26	theme	nut	895:897	arg1	biocomposites					905:917	starch-Brazil nut fiber biocomposites	881:917	starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol	881:1070	To address this knowledge gap, in the present study we evaluated the LCA of starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol.
33486179	0	27	theme	environmental	58:70	arg1	burden					72:77	the environmental burden	54:77	the environmental burden of starch-Brazil nut fiber biocomposite production	54:128	Influence of the source of starch and plasticizers on the environmental burden of starch-Brazil nut fiber biocomposite production: A life cycle assessment approach.
33486179	5	28	theme	biocomposites	905:917	arg1	LCA					874:876	the LCA	870:876	the LCA of starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol	870:1070	To address this knowledge gap, in the present study we evaluated the LCA of starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol.
33486179	9	29	from	glycerol	1558:1565	arg1	categories					1575:1584	most categories	1570:1584	most categories	1570:1584	Sorbitol was found to be many times more impacting than glycerol in most categories, which is due to the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol.
33486179	0	30	from	Influence	0:8	arg1	burden					72:77	the environmental burden	54:77	the environmental burden of starch-Brazil nut fiber biocomposite production	54:128	Influence of the source of starch and plasticizers on the environmental burden of starch-Brazil nut fiber biocomposite production: A life cycle assessment approach.
33486179	8	31	theme	differences	1363:1373	arg1	irrigation					1425:1434	irrigation	1425:1434	irrigation	1425:1434	The main drivers of these differences were the multiple agricultural practices, such as irrigation and fertilization, and the crop efficiency for starch extraction.
33486179	8	31	theme	differences	1363:1373	arg1	practices					1406:1414	the multiple agricultural practices	1380:1414	the multiple agricultural practices	1380:1414	The main drivers of these differences were the multiple agricultural practices, such as irrigation and fertilization, and the crop efficiency for starch extraction.
33486179	8	31	theme	differences	1363:1373	arg1	drivers					1346:1352	The main drivers	1337:1352	The main drivers of these differences	1337:1373	The main drivers of these differences were the multiple agricultural practices, such as irrigation and fertilization, and the crop efficiency for starch extraction.
33486179	8	31	theme	differences	1363:1373	arg1	fertilization					1440:1452	fertilization	1440:1452	fertilization	1440:1452	The main drivers of these differences were the multiple agricultural practices, such as irrigation and fertilization, and the crop efficiency for starch extraction.
33486179	2	32	theme	post-consumer	389:401	arg1	stage					414:418	the post-consumer (disposal) stage	385:418	the post-consumer (disposal) stage	385:418	Owed to their biodegradability, biopolymers are generally regarded as eco-friendly during the post-consumer (disposal) stage.
33486179	0	33	theme	nut	96:98	arg1	production					119:128	starch-Brazil nut fiber biocomposite production	82:128	starch-Brazil nut fiber biocomposite production	82:128	Influence of the source of starch and plasticizers on the environmental burden of starch-Brazil nut fiber biocomposite production: A life cycle assessment approach.
33486179	11	34	dep	limitations	1881:1891	arg1	The					1877:1879	The	1877:1879	The	1877:1879	The limitations, applications, and significance of the results were discussed.
33486179	9	35	theme	impacting	1543:1551	arg1	times					1532:1536	many times	1527:1536	many times more impacting than glycerol in most categories, which is due to the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol	1527:1719	Sorbitol was found to be many times more impacting than glycerol in most categories, which is due to the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol.
33486179	8	36	theme	agricultural	1393:1404	arg1	irrigation					1425:1434	irrigation	1425:1434	irrigation	1425:1434	The main drivers of these differences were the multiple agricultural practices, such as irrigation and fertilization, and the crop efficiency for starch extraction.
33486179	8	36	theme	agricultural	1393:1404	arg1	drivers					1346:1352	The main drivers	1337:1352	The main drivers of these differences	1337:1373	The main drivers of these differences were the multiple agricultural practices, such as irrigation and fertilization, and the crop efficiency for starch extraction.
33486179	8	36	theme	agricultural	1393:1404	arg1	practices					1406:1414	the multiple agricultural practices	1380:1414	the multiple agricultural practices	1380:1414	The main drivers of these differences were the multiple agricultural practices, such as irrigation and fertilization, and the crop efficiency for starch extraction.
33486179	8	36	theme	agricultural	1393:1404	arg1	fertilization					1440:1452	fertilization	1440:1452	fertilization	1440:1452	The main drivers of these differences were the multiple agricultural practices, such as irrigation and fertilization, and the crop efficiency for starch extraction.
33486179	5	37	theme	different	951:959	arg1	sources					961:967	three different sources	945:967	three different sources	945:967	To address this knowledge gap, in the present study we evaluated the LCA of starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol.
33486179	5	37	theme	different	951:959	arg1	potato					977:982	Andean potato	970:982	Andean potato	970:982	To address this knowledge gap, in the present study we evaluated the LCA of starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol.
33486179	5	37	theme	different	951:959	arg1	potato					1001:1006	sweet potato	995:1006	sweet potato	995:1006	To address this knowledge gap, in the present study we evaluated the LCA of starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol.
33486179	5	37	theme	different	951:959	arg1	corn					985:988	corn	985:988	corn	985:988	To address this knowledge gap, in the present study we evaluated the LCA of starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol.
33486179	3	38	dep	undergo	521:527	arg1	reflect					536:542	reflect	536:542	undergo better reflect the sustainable nature of these materials	521:584	However, the environmental burden of the many production processes biopolymers and their components undergo better reflect the sustainable nature of these materials.
33486179	2	39	theme	disposal	404:411	arg1	stage					414:418	the post-consumer (disposal) stage	385:418	the post-consumer (disposal) stage	385:418	Owed to their biodegradability, biopolymers are generally regarded as eco-friendly during the post-consumer (disposal) stage.
33486179	3	40	theme	environmental	434:446	arg1	burden					448:453	the environmental burden	430:453	the environmental burden of the many production processes biopolymers and their components	430:519	However, the environmental burden of the many production processes biopolymers and their components undergo better reflect the sustainable nature of these materials.
33486179	3	41	theme	processes	478:486	arg1	biopolymers					488:498	the many production processes biopolymers	458:498	the many production processes biopolymers	458:498	However, the environmental burden of the many production processes biopolymers and their components undergo better reflect the sustainable nature of these materials.
33486179	7	42	theme	production	1307:1316	arg1	load					1295:1298	the environmental load	1277:1298	the environmental load of the production of the composites	1277:1334	The type of starch and plasticizer significantly influenced the environmental load of the production of the composites.
33486179	3	43	theme	many	462:465	arg1	biopolymers					488:498	the many production processes biopolymers	458:498	the many production processes biopolymers	458:498	However, the environmental burden of the many production processes biopolymers and their components undergo better reflect the sustainable nature of these materials.
33486179	1	44	theme	bio-based	209:217	arg1	polymers					219:226	bio-based polymers	209:226	bio-based polymers	209:226	Amidst the global plastic pollution crisis, bio-based polymers have been proposed as a potential substitute to tackle this issue.
33486179	1	44	theme	bio-based	209:217	arg1	potential					252:260	a potential	250:260	a potential substitute to tackle this issue	250:292	Amidst the global plastic pollution crisis, bio-based polymers have been proposed as a potential substitute to tackle this issue.
33486179	9	45	theme	sorbitol	1629:1636	arg1	processing					1615:1624	the complex processing	1603:1624	the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol	1603:1719	Sorbitol was found to be many times more impacting than glycerol in most categories, which is due to the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol.
33486179	6	46	dep	impacting	1137:1145	arg1	less					1132:1135	less	1132:1135	less	1132:1135	Results indicated that the starch-based biocomposites were less impacting than conventional PLA-Brazil nut fiber and PP-glass fiber composites.
33486179	9	47	with	biocomposites	1658:1670	arg1	properties					1696:1705	similar mechanical properties	1677:1705	similar mechanical properties than glycerol	1677:1719	Sorbitol was found to be many times more impacting than glycerol in most categories, which is due to the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol.
33486179	9	48	theme	high	1642:1645	arg1	content					1647:1653	high content	1642:1653	high content in biocomposites with similar mechanical properties than glycerol	1642:1719	Sorbitol was found to be many times more impacting than glycerol in most categories, which is due to the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol.
33486179	7	49	theme	environmental	1281:1293	arg1	load					1295:1298	the environmental load	1277:1298	the environmental load of the production of the composites	1277:1334	The type of starch and plasticizer significantly influenced the environmental load of the production of the composites.
33486179	4	50	theme	Cycle	624:628	arg1	LCA					642:644	LCA	642:644	LCA	642:644	Previous studies evaluating the Life Cycle Assessment (LCA) of starch-based composites have focused on commercially available starches, although other non-conventional starches can also be used to produce biopolymers.
33486179	4	50	theme	Cycle	624:628	arg1	Assessment					630:639	the Life Cycle Assessment	615:639	the Life Cycle Assessment (LCA) of starch-based composites	615:672	Previous studies evaluating the Life Cycle Assessment (LCA) of starch-based composites have focused on commercially available starches, although other non-conventional starches can also be used to produce biopolymers.
33486179	10	51	theme	nut	1743:1745	arg1	fibers					1747:1752	Brazil nut fibers	1736:1752	Brazil nut fibers	1736:1752	Additionally, Brazil nut fibers are presented as an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin.
33486179	10	51	theme	nut	1743:1745	arg1	filler					1810:1815	an eco-friendly and low-burden natural filler	1771:1815	an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin	1771:1874	Additionally, Brazil nut fibers are presented as an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin.
33486179	10	52	theme	eco-friendly	1774:1785	arg1	fibers					1747:1752	Brazil nut fibers	1736:1752	Brazil nut fibers	1736:1752	Additionally, Brazil nut fibers are presented as an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin.
33486179	10	52	theme	eco-friendly	1774:1785	arg1	filler					1810:1815	an eco-friendly and low-burden natural filler	1771:1815	an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin	1771:1874	Additionally, Brazil nut fibers are presented as an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin.
33486179	6	53	theme	PP-glass	1190:1197	arg1	composites					1205:1214	conventional PLA-Brazil nut fiber and PP-glass fiber composites	1152:1214	composites	1205:1214	Results indicated that the starch-based biocomposites were less impacting than conventional PLA-Brazil nut fiber and PP-glass fiber composites.
33486179	9	54	theme	mechanical	1685:1694	arg1	properties					1696:1705	similar mechanical properties	1677:1705	similar mechanical properties than glycerol	1677:1719	Sorbitol was found to be many times more impacting than glycerol in most categories, which is due to the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol.
33486179	4	55	theme	starch-based	650:661	arg1	composites					663:672	starch-based composites	650:672	starch-based composites	650:672	Previous studies evaluating the Life Cycle Assessment (LCA) of starch-based composites have focused on commercially available starches, although other non-conventional starches can also be used to produce biopolymers.
33486179	7	56	theme	starch	1229:1234	arg1	type					1221:1224	The type	1217:1224	The type of starch and plasticizer	1217:1250	The type of starch and plasticizer significantly influenced the environmental load of the production of the composites.
33486179	6	57	theme	PLA-Brazil	1165:1174	arg1	fiber					1180:1184	conventional PLA-Brazil nut fiber and PP-glass fiber composites	1152:1214	fiber	1180:1184	Results indicated that the starch-based biocomposites were less impacting than conventional PLA-Brazil nut fiber and PP-glass fiber composites.
33486179	0	58	theme	production	119:128	arg1	burden					72:77	the environmental burden	54:77	the environmental burden of starch-Brazil nut fiber biocomposite production	54:128	Influence of the source of starch and plasticizers on the environmental burden of starch-Brazil nut fiber biocomposite production: A life cycle assessment approach.
33486179	10	59	theme	low-burden	1791:1800	arg1	fibers					1747:1752	Brazil nut fibers	1736:1752	Brazil nut fibers	1736:1752	Additionally, Brazil nut fibers are presented as an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin.
33486179	10	59	theme	low-burden	1791:1800	arg1	filler					1810:1815	an eco-friendly and low-burden natural filler	1771:1815	an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin	1771:1874	Additionally, Brazil nut fibers are presented as an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin.
33486179	1	60	theme	plastic	183:189	arg1	crisis					201:206	the global plastic pollution crisis	172:206	the global plastic pollution crisis	172:206	Amidst the global plastic pollution crisis, bio-based polymers have been proposed as a potential substitute to tackle this issue.
33486179	10	61	theme	natural	1802:1808	arg1	fibers					1747:1752	Brazil nut fibers	1736:1752	Brazil nut fibers	1736:1752	Additionally, Brazil nut fibers are presented as an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin.
33486179	10	61	theme	natural	1802:1808	arg1	filler					1810:1815	an eco-friendly and low-burden natural filler	1771:1815	an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin	1771:1874	Additionally, Brazil nut fibers are presented as an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin.
33486179	10	62	theme	easy	1830:1833	arg1	processing					1835:1844	their easy processing	1824:1844	their easy processing	1824:1844	Additionally, Brazil nut fibers are presented as an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin.
33486179	9	63	from	biocomposites	1658:1670	arg1	processing					1615:1624	the complex processing	1603:1624	the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol	1603:1719	Sorbitol was found to be many times more impacting than glycerol in most categories, which is due to the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol.
33486179	8	64	theme	starch	1483:1488	arg1	extraction					1490:1499	starch extraction	1483:1499	starch extraction	1483:1499	The main drivers of these differences were the multiple agricultural practices, such as irrigation and fertilization, and the crop efficiency for starch extraction.
33486179	0	65	theme	cycle	138:142	arg1	approach					155:162	A life cycle assessment approach	131:162	Influence of the source of starch and plasticizers on the environmental burden of starch-Brazil nut fiber biocomposite production: A life cycle assessment approach.	0:163	Influence of the source of starch and plasticizers on the environmental burden of starch-Brazil nut fiber biocomposite production: A life cycle assessment approach.
33486179	9	66	from	content	1647:1653	arg1	biocomposites					1658:1670	biocomposites	1658:1670	biocomposites with similar mechanical properties than glycerol	1658:1719	Sorbitol was found to be many times more impacting than glycerol in most categories, which is due to the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol.
33486179	10	67	theme	waste	1863:1867	arg1	origin					1869:1874	agricultural waste origin	1850:1874	agricultural waste origin	1850:1874	Additionally, Brazil nut fibers are presented as an eco-friendly and low-burden natural filler due to their easy processing and agricultural waste origin.
33486179	4	68	theme	other	732:736	arg1	starches					755:762	other non-conventional starches	732:762	other non-conventional starches	732:762	Previous studies evaluating the Life Cycle Assessment (LCA) of starch-based composites have focused on commercially available starches, although other non-conventional starches can also be used to produce biopolymers.
33486179	5	69	theme	starch-Brazil	881:893	arg1	biocomposites					905:917	starch-Brazil nut fiber biocomposites	881:917	starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol	881:1070	To address this knowledge gap, in the present study we evaluated the LCA of starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol.
33486179	5	70	theme	fiber	899:903	arg1	biocomposites					905:917	starch-Brazil nut fiber biocomposites	881:917	starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol	881:1070	To address this knowledge gap, in the present study we evaluated the LCA of starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol.
33486179	4	71	theme	available	703:711	arg1	starches					713:720	commercially available starches	690:720	commercially available starches	690:720	Previous studies evaluating the Life Cycle Assessment (LCA) of starch-based composites have focused on commercially available starches, although other non-conventional starches can also be used to produce biopolymers.
33486179	0	72	theme	starch-Brazil	82:94	arg1	production					119:128	starch-Brazil nut fiber biocomposite production	82:128	starch-Brazil nut fiber biocomposite production	82:128	Influence of the source of starch and plasticizers on the environmental burden of starch-Brazil nut fiber biocomposite production: A life cycle assessment approach.
33486179	5	73	theme	Andean	970:975	arg1	sources					961:967	three different sources	945:967	three different sources	945:967	To address this knowledge gap, in the present study we evaluated the LCA of starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol.
33486179	5	73	theme	Andean	970:975	arg1	potato					977:982	Andean potato	970:982	Andean potato	970:982	To address this knowledge gap, in the present study we evaluated the LCA of starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol.
33486179	0	74	theme	fiber	100:104	arg1	production					119:128	starch-Brazil nut fiber biocomposite production	82:128	starch-Brazil nut fiber biocomposite production	82:128	Influence of the source of starch and plasticizers on the environmental burden of starch-Brazil nut fiber biocomposite production: A life cycle assessment approach.
33486179	1	75	theme	pollution	191:199	arg1	crisis					201:206	the global plastic pollution crisis	172:206	the global plastic pollution crisis	172:206	Amidst the global plastic pollution crisis, bio-based polymers have been proposed as a potential substitute to tackle this issue.
33486179	9	76	theme	most	1570:1573	arg1	categories					1575:1584	most categories	1570:1584	most categories	1570:1584	Sorbitol was found to be many times more impacting than glycerol in most categories, which is due to the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol.
33486179	11	77	theme	results	1932:1938	arg1	applications					1894:1905	applications	1894:1905	applications	1894:1905	The limitations, applications, and significance of the results were discussed.
33486179	11	77	theme	results	1932:1938	arg1	significance					1912:1923	significance	1912:1923	significance	1912:1923	The limitations, applications, and significance of the results were discussed.
33486179	11	77	theme	results	1932:1938	arg1	limitations					1881:1891	limitations	1881:1891	limitations	1881:1891	The limitations, applications, and significance of the results were discussed.
33486179	5	78	theme	knowledge	821:829	arg1	gap					831:833	this knowledge gap	816:833	this knowledge gap	816:833	To address this knowledge gap, in the present study we evaluated the LCA of starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol.
33486179	9	79	from	sorbitol	1629:1636	arg1	biocomposites					1658:1670	biocomposites	1658:1670	biocomposites with similar mechanical properties than glycerol	1658:1719	Sorbitol was found to be many times more impacting than glycerol in most categories, which is due to the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol.
33486179	5	80	dep	biocomposites	905:917	arg1	applying					1013:1020	applying	1013:1020	applying two different plasticizers, glycerol and sorbitol	1013:1070	To address this knowledge gap, in the present study we evaluated the LCA of starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol.
33486179	5	80	dep	biocomposites	905:917	arg1	prepared					919:926	prepared	919:926	prepared with starch from three different sources, Andean potato, corn, and sweet potato,	919:1007	To address this knowledge gap, in the present study we evaluated the LCA of starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol.
33486179	0	81	theme	source	17:22	arg1	Influence					0:8	Influence	0:8	Influence of the source of starch and plasticizers on the environmental burden of starch-Brazil nut fiber biocomposite production: A life cycle assessment approach.	0:163	Influence of the source of starch and plasticizers on the environmental burden of starch-Brazil nut fiber biocomposite production: A life cycle assessment approach.
33486179	9	82	from	processing	1615:1624	arg1	biocomposites					1658:1670	biocomposites	1658:1670	biocomposites with similar mechanical properties than glycerol	1658:1719	Sorbitol was found to be many times more impacting than glycerol in most categories, which is due to the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol.
33486179	7	83	theme	composites	1325:1334	arg1	production					1307:1316	the production	1303:1316	the production of the composites	1303:1334	The type of starch and plasticizer significantly influenced the environmental load of the production of the composites.
33486179	3	84	theme	components	510:519	arg1	burden					448:453	the environmental burden	430:453	the environmental burden of the many production processes biopolymers and their components	430:519	However, the environmental burden of the many production processes biopolymers and their components undergo better reflect the sustainable nature of these materials.
33486179	0	85	theme	starch	27:32	arg1	source					17:22	the source	13:22	the source of starch and plasticizers	13:49	Influence of the source of starch and plasticizers on the environmental burden of starch-Brazil nut fiber biocomposite production: A life cycle assessment approach.
33486179	0	86	theme	plasticizers	38:49	arg1	source					17:22	the source	13:22	the source of starch and plasticizers	13:49	Influence of the source of starch and plasticizers on the environmental burden of starch-Brazil nut fiber biocomposite production: A life cycle assessment approach.
33486179	9	87	theme	complex	1607:1613	arg1	processing					1615:1624	the complex processing	1603:1624	the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol	1603:1719	Sorbitol was found to be many times more impacting than glycerol in most categories, which is due to the complex processing of sorbitol and high content in biocomposites with similar mechanical properties than glycerol.
33486179	3	88	theme	biopolymers	488:498	arg1	burden					448:453	the environmental burden	430:453	the environmental burden of the many production processes biopolymers and their components	430:519	However, the environmental burden of the many production processes biopolymers and their components undergo better reflect the sustainable nature of these materials.
33486179	0	89	dep	Influence	0:8	arg1	approach					155:162	A life cycle assessment approach	131:162	Influence of the source of starch and plasticizers on the environmental burden of starch-Brazil nut fiber biocomposite production: A life cycle assessment approach.	0:163	Influence of the source of starch and plasticizers on the environmental burden of starch-Brazil nut fiber biocomposite production: A life cycle assessment approach.
33486179	4	90	theme	Life	619:622	arg1	LCA					642:644	LCA	642:644	LCA	642:644	Previous studies evaluating the Life Cycle Assessment (LCA) of starch-based composites have focused on commercially available starches, although other non-conventional starches can also be used to produce biopolymers.
33486179	4	90	theme	Life	619:622	arg1	Assessment					630:639	the Life Cycle Assessment	615:639	the Life Cycle Assessment (LCA) of starch-based composites	615:672	Previous studies evaluating the Life Cycle Assessment (LCA) of starch-based composites have focused on commercially available starches, although other non-conventional starches can also be used to produce biopolymers.
33486179	3	91	theme	production	467:476	arg1	biopolymers					488:498	the many production processes biopolymers	458:498	the many production processes biopolymers	458:498	However, the environmental burden of the many production processes biopolymers and their components undergo better reflect the sustainable nature of these materials.
33486179	4	92	used	used	776:779	arg2	starches					755:762	other non-conventional starches	732:762	other non-conventional starches	732:762	Previous studies evaluating the Life Cycle Assessment (LCA) of starch-based composites have focused on commercially available starches, although other non-conventional starches can also be used to produce biopolymers.
33486179	5	93	theme	sweet	995:999	arg1	sources					961:967	three different sources	945:967	three different sources	945:967	To address this knowledge gap, in the present study we evaluated the LCA of starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol.
33486179	5	93	theme	sweet	995:999	arg1	potato					1001:1006	sweet potato	995:1006	sweet potato	995:1006	To address this knowledge gap, in the present study we evaluated the LCA of starch-Brazil nut fiber biocomposites prepared with starch from three different sources, Andean potato, corn, and sweet potato, and applying two different plasticizers, glycerol and sorbitol.
33486179	6	94	theme	starch-based	1100:1111	arg1	biocomposites					1113:1125	the starch-based biocomposites	1096:1125	the starch-based biocomposites	1096:1125	Results indicated that the starch-based biocomposites were less impacting than conventional PLA-Brazil nut fiber and PP-glass fiber composites.
33486179	6	94	theme	starch-based	1100:1111	arg1	impacting					1137:1145	impacting	1137:1145	impacting	1137:1145	Results indicated that the starch-based biocomposites were less impacting than conventional PLA-Brazil nut fiber and PP-glass fiber composites.
34299003	0	0	theme	Antibacterial	97:109	arg1	Properties					126:135	Enhanced Antibacterial and Mechanical Properties	88:135	Enhanced Antibacterial and Mechanical Properties	88:135	Fabrication of Paper Sheets Coatings Based on Chitosan/Bacterial Nanocellulose/ZnO with Enhanced Antibacterial and Mechanical Properties.
34299003	5	1	theme	standard	764:771	arg1	ISO					755:757	the ISO	751:757	the ISO 5270 standard	751:771	The mechanical properties of the paper sheets were measured by comparing tearing resistance, tensile strength, and bursting strength according to the ISO 5270 standard.
34299003	3	2	theme	nanofibers	493:502	arg1	formation					480:488	the formation	476:488	the formation of nanofibers	476:502	The proposed processing of bacterial cellulose resulted in the formation of nanofibers.
34299003	7	3	theme	food	1043:1046	arg1	applications					1058:1069	food packaging applications	1043:1069	food packaging applications	1043:1069	Therefore, the proposed composition is an interesting multifunctional mixture for coatings in food packaging applications.
34299003	1	4	theme	antibacterial	246:258	arg1	activity					275:282	boosted antibacterial and mechanical activity	238:282	boosted antibacterial and mechanical activity	238:282	Here, we designed paper sheets coated with chitosan, bacterial cellulose (nanofibers), and ZnO with boosted antibacterial and mechanical activity.
34299003	0	5	theme	Enhanced	88:95	arg1	Properties					126:135	Enhanced Antibacterial and Mechanical Properties	88:135	Enhanced Antibacterial and Mechanical Properties	88:135	Fabrication of Paper Sheets Coatings Based on Chitosan/Bacterial Nanocellulose/ZnO with Enhanced Antibacterial and Mechanical Properties.
34299003	0	6	theme	Mechanical	115:124	arg1	Properties					126:135	Enhanced Antibacterial and Mechanical Properties	88:135	Enhanced Antibacterial and Mechanical Properties	88:135	Fabrication of Paper Sheets Coatings Based on Chitosan/Bacterial Nanocellulose/ZnO with Enhanced Antibacterial and Mechanical Properties.
34299003	4	7	theme	Antimicrobial	505:517	arg1	behavior					519:526	Antimicrobial behavior	505:526	Antimicrobial behavior	505:526	Antimicrobial behavior was tested using E. coli ATCC® 25922™ following the ASTM E2149-13a standard.
34299003	6	8	theme	antibacterial	806:818	arg1	response					820:827	an increased antibacterial response	793:827	an increased antibacterial response (assigned to the combination of chitosan and ZnO, independent of its shape and size)	793:912	The results showed an increased antibacterial response (assigned to the combination of chitosan and ZnO, independent of its shape and size) and boosted mechanical properties.
34299003	1	9	theme	mechanical	264:273	arg1	activity					275:282	boosted antibacterial and mechanical activity	238:282	boosted antibacterial and mechanical activity	238:282	Here, we designed paper sheets coated with chitosan, bacterial cellulose (nanofibers), and ZnO with boosted antibacterial and mechanical activity.
34299003	5	10	theme	mechanical	609:618	arg1	properties					620:629	The mechanical properties	605:629	The mechanical properties of the paper sheets	605:649	The mechanical properties of the paper sheets were measured by comparing tearing resistance, tensile strength, and bursting strength according to the ISO 5270 standard.
34299003	6	11	theme	increased	796:804	arg1	response					820:827	an increased antibacterial response	793:827	an increased antibacterial response (assigned to the combination of chitosan and ZnO, independent of its shape and size)	793:912	The results showed an increased antibacterial response (assigned to the combination of chitosan and ZnO, independent of its shape and size) and boosted mechanical properties.
34299003	3	12	theme	proposed	421:428	arg1	processing					430:439	The proposed processing	417:439	The proposed processing of bacterial cellulose	417:462	The proposed processing of bacterial cellulose resulted in the formation of nanofibers.
34299003	6	13	theme	independent	879:889	arg1	chitosan					861:868	chitosan	861:868	chitosan	861:868	The results showed an increased antibacterial response (assigned to the combination of chitosan and ZnO, independent of its shape and size) and boosted mechanical properties.
34299003	5	14	theme	paper	638:642	arg1	sheets					644:649	the paper sheets	634:649	the paper sheets	634:649	The mechanical properties of the paper sheets were measured by comparing tearing resistance, tensile strength, and bursting strength according to the ISO 5270 standard.
34299003	1	15	theme	bacterial	191:199	arg1	nanofibers					212:221	nanofibers	212:221	nanofibers	212:221	Here, we designed paper sheets coated with chitosan, bacterial cellulose (nanofibers), and ZnO with boosted antibacterial and mechanical activity.
34299003	1	15	theme	bacterial	191:199	arg1	cellulose					201:209	bacterial cellulose	191:209	bacterial cellulose (nanofibers)	191:222	Here, we designed paper sheets coated with chitosan, bacterial cellulose (nanofibers), and ZnO with boosted antibacterial and mechanical activity.
34299003	4	16	theme	ATCC®	553:557	arg1	25922™					559:564	E. coli ATCC® 25922™	545:564	E. coli ATCC® 25922™ following the ASTM E2149-13a standard	545:602	Antimicrobial behavior was tested using E. coli ATCC® 25922™ following the ASTM E2149-13a standard.
34299003	5	17	theme	sheets	644:649	arg1	properties					620:629	The mechanical properties	605:629	The mechanical properties of the paper sheets	605:649	The mechanical properties of the paper sheets were measured by comparing tearing resistance, tensile strength, and bursting strength according to the ISO 5270 standard.
34299003	0	18	theme	Sheets	21:26	arg1	Coatings					28:35	Paper Sheets Coatings	15:35	Paper Sheets Coatings	15:35	Fabrication of Paper Sheets Coatings Based on Chitosan/Bacterial Nanocellulose/ZnO with Enhanced Antibacterial and Mechanical Properties.
34299003	2	19	dep	sizes/shapes	353:364	arg1	rods					371:374	rods	371:374	rods	371:374	We investigated the compositions, with ZnO exhibiting two different sizes/shapes: (1) rods and (2) irregular sphere-like particles.
34299003	2	19	dep	sizes/shapes	353:364	arg1	particles					406:414	irregular sphere-like particles	384:414	(2) irregular sphere-like particles	380:414	We investigated the compositions, with ZnO exhibiting two different sizes/shapes: (1) rods and (2) irregular sphere-like particles.
34299003	3	20	theme	cellulose	454:462	arg1	processing					430:439	The proposed processing	417:439	The proposed processing of bacterial cellulose	417:462	The proposed processing of bacterial cellulose resulted in the formation of nanofibers.
34299003	0	21	theme	Paper	15:19	arg1	Coatings					28:35	Paper Sheets Coatings	15:35	Paper Sheets Coatings	15:35	Fabrication of Paper Sheets Coatings Based on Chitosan/Bacterial Nanocellulose/ZnO with Enhanced Antibacterial and Mechanical Properties.
34299003	6	22	theme	ZnO	874:876	arg1	combination					846:856	the combination	842:856	the combination of chitosan and ZnO, independent of its shape and size	842:911	The results showed an increased antibacterial response (assigned to the combination of chitosan and ZnO, independent of its shape and size) and boosted mechanical properties.
34299003	6	23	theme	size	908:911	arg1	independent					879:889	independent	879:889	independent	879:889	The results showed an increased antibacterial response (assigned to the combination of chitosan and ZnO, independent of its shape and size) and boosted mechanical properties.
34299003	2	24	theme	sphere-like	394:404	arg1	particles					406:414	irregular sphere-like particles	384:414	(2) irregular sphere-like particles	380:414	We investigated the compositions, with ZnO exhibiting two different sizes/shapes: (1) rods and (2) irregular sphere-like particles.
34299003	6	25	dep	response	820:827	arg1	assigned					830:837	assigned	830:837	assigned to the combination of chitosan and ZnO, independent of its shape and size	830:911	The results showed an increased antibacterial response (assigned to the combination of chitosan and ZnO, independent of its shape and size) and boosted mechanical properties.
34299003	2	26	theme	irregular	384:392	arg1	particles					406:414	irregular sphere-like particles	384:414	(2) irregular sphere-like particles	380:414	We investigated the compositions, with ZnO exhibiting two different sizes/shapes: (1) rods and (2) irregular sphere-like particles.
34299003	0	27	theme	Coatings	28:35	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of Paper Sheets Coatings	0:35	Fabrication of Paper Sheets Coatings Based on Chitosan/Bacterial Nanocellulose/ZnO with Enhanced Antibacterial and Mechanical Properties.
34299003	5	28	theme	bursting	720:727	arg1	strength					729:736	bursting strength	720:736	bursting strength	720:736	The mechanical properties of the paper sheets were measured by comparing tearing resistance, tensile strength, and bursting strength according to the ISO 5270 standard.
34299003	6	29	theme	chitosan	861:868	arg1	combination					846:856	the combination	842:856	the combination of chitosan and ZnO, independent of its shape and size	842:911	The results showed an increased antibacterial response (assigned to the combination of chitosan and ZnO, independent of its shape and size) and boosted mechanical properties.
34299003	3	30	theme	bacterial	444:452	arg1	cellulose					454:462	bacterial cellulose	444:462	bacterial cellulose	444:462	The proposed processing of bacterial cellulose resulted in the formation of nanofibers.
34299003	6	31	theme	mechanical	926:935	arg1	properties					937:946	mechanical properties	926:946	mechanical properties	926:946	The results showed an increased antibacterial response (assigned to the combination of chitosan and ZnO, independent of its shape and size) and boosted mechanical properties.
34299003	5	32	theme	tearing	678:684	arg1	resistance					686:695	tearing resistance	678:695	tearing resistance	678:695	The mechanical properties of the paper sheets were measured by comparing tearing resistance, tensile strength, and bursting strength according to the ISO 5270 standard.
34299003	2	33	dep	particles	406:414	arg1	2					381:381	2	381:381	2	381:381	We investigated the compositions, with ZnO exhibiting two different sizes/shapes: (1) rods and (2) irregular sphere-like particles.
34299003	6	34	theme	shape	898:902	arg1	independent					879:889	independent	879:889	independent	879:889	The results showed an increased antibacterial response (assigned to the combination of chitosan and ZnO, independent of its shape and size) and boosted mechanical properties.
34299003	4	35	theme	E.	545:546	arg1	25922™					559:564	E. coli ATCC® 25922™	545:564	E. coli ATCC® 25922™ following the ASTM E2149-13a standard	545:602	Antimicrobial behavior was tested using E. coli ATCC® 25922™ following the ASTM E2149-13a standard.
34299003	7	36	theme	packaging	1048:1056	arg1	applications					1058:1069	food packaging applications	1043:1069	food packaging applications	1043:1069	Therefore, the proposed composition is an interesting multifunctional mixture for coatings in food packaging applications.
34299003	7	37	theme	interesting	991:1001	arg1	mixture					1019:1025	an interesting multifunctional mixture	988:1025	an interesting multifunctional mixture for coatings in food packaging applications	988:1069	Therefore, the proposed composition is an interesting multifunctional mixture for coatings in food packaging applications.
34299003	7	37	theme	interesting	991:1001	arg1	composition					973:983	the proposed composition	960:983	the proposed composition	960:983	Therefore, the proposed composition is an interesting multifunctional mixture for coatings in food packaging applications.
34299003	2	38	theme	different	343:351	arg1	sizes/shapes					353:364	two different sizes/shapes	339:364	two different sizes/shapes: (1) rods and (2) irregular sphere-like particles	339:414	We investigated the compositions, with ZnO exhibiting two different sizes/shapes: (1) rods and (2) irregular sphere-like particles.
34299003	7	39	theme	multifunctional	1003:1017	arg1	mixture					1019:1025	an interesting multifunctional mixture	988:1025	an interesting multifunctional mixture for coatings in food packaging applications	988:1069	Therefore, the proposed composition is an interesting multifunctional mixture for coatings in food packaging applications.
34299003	7	39	theme	multifunctional	1003:1017	arg1	composition					973:983	the proposed composition	960:983	the proposed composition	960:983	Therefore, the proposed composition is an interesting multifunctional mixture for coatings in food packaging applications.
34299003	7	40	from	coatings	1031:1038	arg1	applications					1058:1069	food packaging applications	1043:1069	food packaging applications	1043:1069	Therefore, the proposed composition is an interesting multifunctional mixture for coatings in food packaging applications.
34299003	5	41	theme	tensile	698:704	arg1	strength					706:713	tensile strength	698:713	tensile strength	698:713	The mechanical properties of the paper sheets were measured by comparing tearing resistance, tensile strength, and bursting strength according to the ISO 5270 standard.
34299003	2	42	dep	rods	371:374	arg1	1					368:368	1	368:368	1	368:368	We investigated the compositions, with ZnO exhibiting two different sizes/shapes: (1) rods and (2) irregular sphere-like particles.
34299003	1	43	theme	paper	156:160	arg1	sheets					162:167	paper sheets	156:167	paper sheets coated with chitosan, bacterial cellulose (nanofibers), and ZnO with boosted antibacterial and mechanical activity	156:282	Here, we designed paper sheets coated with chitosan, bacterial cellulose (nanofibers), and ZnO with boosted antibacterial and mechanical activity.
34299003	4	44	theme	standard	595:602	arg1	ASTM					580:583	the ASTM	576:583	the ASTM E2149-13a standard	576:602	Antimicrobial behavior was tested using E. coli ATCC® 25922™ following the ASTM E2149-13a standard.
34299003	4	45	dep	E.	545:546	arg1	coli					548:551	coli	548:551	coli	548:551	Antimicrobial behavior was tested using E. coli ATCC® 25922™ following the ASTM E2149-13a standard.
34299003	7	46	theme	proposed	964:971	arg1	mixture					1019:1025	an interesting multifunctional mixture	988:1025	an interesting multifunctional mixture for coatings in food packaging applications	988:1069	Therefore, the proposed composition is an interesting multifunctional mixture for coatings in food packaging applications.
34299003	7	46	theme	proposed	964:971	arg1	composition					973:983	the proposed composition	960:983	the proposed composition	960:983	Therefore, the proposed composition is an interesting multifunctional mixture for coatings in food packaging applications.
34299003	1	47	theme	boosted	238:244	arg1	activity					275:282	boosted antibacterial and mechanical activity	238:282	boosted antibacterial and mechanical activity	238:282	Here, we designed paper sheets coated with chitosan, bacterial cellulose (nanofibers), and ZnO with boosted antibacterial and mechanical activity.
33253535	1	0	theme	reliable	151:158	arg1	materials					207:215	reliable, sustainable, and cost-effective antibacterial materials	151:215	reliable, sustainable, and cost-effective antibacterial materials	151:215	In modern society, there is a constant need for developing reliable, sustainable, and cost-effective antibacterial materials.
33253535	4	1	theme	loading	615:621	arg1	levels					623:628	higher lysozyme loading levels	599:628	higher lysozyme loading levels (ca. 10 wt %)	599:642	Although at higher lysozyme loading levels (ca. 10 wt %), composite films lost their characteristic chiral nematic structuring, these films demonstrated improved mechanical properties and antibacterial activity with respect to CNC-only films, regardless of lysozyme aggregation state.
33253535	4	1	theme	loading	615:621	arg1	%					641:641	10 wt %	635:641	10 wt %	635:641	Although at higher lysozyme loading levels (ca. 10 wt %), composite films lost their characteristic chiral nematic structuring, these films demonstrated improved mechanical properties and antibacterial activity with respect to CNC-only films, regardless of lysozyme aggregation state.
33253535	2	2	theme	self-assembly	366:378	arg1	method					336:341	the well-established method	315:341	the well-established method of evaporation-induced self-assembly	315:378	Here, we investigate the preparation of cellulose nanocrystal (CNC)-lysozyme composite films via the well-established method of evaporation-induced self-assembly.
33253535	4	3	theme	lysozyme	606:613	arg1	levels					623:628	higher lysozyme loading levels	599:628	higher lysozyme loading levels (ca. 10 wt %)	599:642	Although at higher lysozyme loading levels (ca. 10 wt %), composite films lost their characteristic chiral nematic structuring, these films demonstrated improved mechanical properties and antibacterial activity with respect to CNC-only films, regardless of lysozyme aggregation state.
33253535	4	3	theme	lysozyme	606:613	arg1	%					641:641	10 wt %	635:641	10 wt %	635:641	Although at higher lysozyme loading levels (ca. 10 wt %), composite films lost their characteristic chiral nematic structuring, these films demonstrated improved mechanical properties and antibacterial activity with respect to CNC-only films, regardless of lysozyme aggregation state.
33253535	4	4	theme	nematic	694:700	arg1	structuring					702:712	their characteristic chiral nematic structuring	666:712	their characteristic chiral nematic structuring	666:712	Although at higher lysozyme loading levels (ca. 10 wt %), composite films lost their characteristic chiral nematic structuring, these films demonstrated improved mechanical properties and antibacterial activity with respect to CNC-only films, regardless of lysozyme aggregation state.
33253535	2	5	theme	-lysozyme	285:293	arg1	films					305:309	cellulose nanocrystal (CNC)-lysozyme composite films	258:309	cellulose nanocrystal (CNC)-lysozyme composite films	258:309	Here, we investigate the preparation of cellulose nanocrystal (CNC)-lysozyme composite films via the well-established method of evaporation-induced self-assembly.
33253535	4	6	theme	antibacterial	775:787	arg1	activity					789:796	antibacterial activity	775:796	antibacterial activity	775:796	Although at higher lysozyme loading levels (ca. 10 wt %), composite films lost their characteristic chiral nematic structuring, these films demonstrated improved mechanical properties and antibacterial activity with respect to CNC-only films, regardless of lysozyme aggregation state.
33253535	3	7	theme	sonicated	500:508	arg1	lysozyme					510:517	sonicated lysozyme	500:517	sonicated lysozyme	500:517	We consider the effects of lysozyme concentration and aggregation state (native lysozyme, lysozyme amyloid fibers, and sonicated lysozyme amyloid fibers) on suspension aggregation and film-forming ability.
33253535	3	8	from	effects	397:403	arg1	aggregation					549:559	suspension aggregation	538:559	suspension aggregation	538:559	We consider the effects of lysozyme concentration and aggregation state (native lysozyme, lysozyme amyloid fibers, and sonicated lysozyme amyloid fibers) on suspension aggregation and film-forming ability.
33253535	3	8	from	effects	397:403	arg1	ability					578:584	film-forming ability	565:584	film-forming ability	565:584	We consider the effects of lysozyme concentration and aggregation state (native lysozyme, lysozyme amyloid fibers, and sonicated lysozyme amyloid fibers) on suspension aggregation and film-forming ability.
33253535	3	9	dep	concentration	417:429	arg1	lysozyme					510:517	sonicated lysozyme	500:517	sonicated lysozyme	500:517	We consider the effects of lysozyme concentration and aggregation state (native lysozyme, lysozyme amyloid fibers, and sonicated lysozyme amyloid fibers) on suspension aggregation and film-forming ability.
33253535	3	9	dep	concentration	417:429	arg1	lysozyme					461:468	native lysozyme	454:468	native lysozyme	454:468	We consider the effects of lysozyme concentration and aggregation state (native lysozyme, lysozyme amyloid fibers, and sonicated lysozyme amyloid fibers) on suspension aggregation and film-forming ability.
33253535	3	9	dep	concentration	417:429	arg1	lysozyme					471:478	lysozyme amyloid fibers	471:493	lysozyme amyloid fibers	471:493	We consider the effects of lysozyme concentration and aggregation state (native lysozyme, lysozyme amyloid fibers, and sonicated lysozyme amyloid fibers) on suspension aggregation and film-forming ability.
33253535	4	10	theme	CNC-only	814:821	arg1	films					823:827	CNC-only films	814:827	CNC-only films	814:827	Although at higher lysozyme loading levels (ca. 10 wt %), composite films lost their characteristic chiral nematic structuring, these films demonstrated improved mechanical properties and antibacterial activity with respect to CNC-only films, regardless of lysozyme aggregation state.
33253535	5	11	theme	other	964:968	arg1	hydrogels					1016:1024	hydrogels	1016:1024	hydrogels	1016:1024	We anticipate that the results presented herein could also contribute to the preparation of other CNC-protein-based materials, including films, hydrogels, and aerogels, with improved mechanical performance and antibacterial activity.
33253535	5	11	theme	other	964:968	arg1	materials					988:996	other CNC-protein-based materials	964:996	other CNC-protein-based materials	964:996	We anticipate that the results presented herein could also contribute to the preparation of other CNC-protein-based materials, including films, hydrogels, and aerogels, with improved mechanical performance and antibacterial activity.
33253535	5	11	theme	other	964:968	arg1	films					1009:1013	films	1009:1013	films	1009:1013	We anticipate that the results presented herein could also contribute to the preparation of other CNC-protein-based materials, including films, hydrogels, and aerogels, with improved mechanical performance and antibacterial activity.
33253535	5	11	theme	other	964:968	arg1	aerogels					1031:1038	aerogels	1031:1038	aerogels	1031:1038	We anticipate that the results presented herein could also contribute to the preparation of other CNC-protein-based materials, including films, hydrogels, and aerogels, with improved mechanical performance and antibacterial activity.
33253535	1	12	theme	sustainable	161:171	arg1	materials					207:215	reliable, sustainable, and cost-effective antibacterial materials	151:215	reliable, sustainable, and cost-effective antibacterial materials	151:215	In modern society, there is a constant need for developing reliable, sustainable, and cost-effective antibacterial materials.
33253535	0	13	theme	Films	53:57	arg1	Assembly					0:7	Assembly	0:7	Assembly of Cellulose Nanocrystal-Lysozyme Composite Films with Varied Lysozyme Morphology.	0:90	Assembly of Cellulose Nanocrystal-Lysozyme Composite Films with Varied Lysozyme Morphology.
33253535	5	14	with	preparation	949:959	arg1	performance					1066:1076	improved mechanical performance	1046:1076	improved mechanical performance	1046:1076	We anticipate that the results presented herein could also contribute to the preparation of other CNC-protein-based materials, including films, hydrogels, and aerogels, with improved mechanical performance and antibacterial activity.
33253535	5	14	with	preparation	949:959	arg1	activity					1096:1103	antibacterial activity	1082:1103	antibacterial activity	1082:1103	We anticipate that the results presented herein could also contribute to the preparation of other CNC-protein-based materials, including films, hydrogels, and aerogels, with improved mechanical performance and antibacterial activity.
33253535	5	15	theme	materials	988:996	arg1	preparation					949:959	the preparation	945:959	the preparation of other CNC-protein-based materials, including films, hydrogels, and aerogels, with improved mechanical performance and antibacterial activity	945:1103	We anticipate that the results presented herein could also contribute to the preparation of other CNC-protein-based materials, including films, hydrogels, and aerogels, with improved mechanical performance and antibacterial activity.
33253535	4	16	theme	chiral	687:692	arg1	structuring					702:712	their characteristic chiral nematic structuring	666:712	their characteristic chiral nematic structuring	666:712	Although at higher lysozyme loading levels (ca. 10 wt %), composite films lost their characteristic chiral nematic structuring, these films demonstrated improved mechanical properties and antibacterial activity with respect to CNC-only films, regardless of lysozyme aggregation state.
33253535	3	17	theme	lysozyme	408:415	arg1	concentration					417:429	lysozyme concentration	408:429	lysozyme concentration	408:429	We consider the effects of lysozyme concentration and aggregation state (native lysozyme, lysozyme amyloid fibers, and sonicated lysozyme amyloid fibers) on suspension aggregation and film-forming ability.
33253535	4	18	theme	improved	740:747	arg1	properties					760:769	improved mechanical properties	740:769	improved mechanical properties	740:769	Although at higher lysozyme loading levels (ca. 10 wt %), composite films lost their characteristic chiral nematic structuring, these films demonstrated improved mechanical properties and antibacterial activity with respect to CNC-only films, regardless of lysozyme aggregation state.
33253535	1	19	theme	cost-effective	178:191	arg1	materials					207:215	reliable, sustainable, and cost-effective antibacterial materials	151:215	reliable, sustainable, and cost-effective antibacterial materials	151:215	In modern society, there is a constant need for developing reliable, sustainable, and cost-effective antibacterial materials.
33253535	0	20	theme	Nanocrystal-Lysozyme	22:41	arg1	Films					53:57	Cellulose Nanocrystal-Lysozyme Composite Films	12:57	Cellulose Nanocrystal-Lysozyme Composite Films	12:57	Assembly of Cellulose Nanocrystal-Lysozyme Composite Films with Varied Lysozyme Morphology.
33253535	5	21	theme	CNC-protein-based	970:986	arg1	hydrogels					1016:1024	hydrogels	1016:1024	hydrogels	1016:1024	We anticipate that the results presented herein could also contribute to the preparation of other CNC-protein-based materials, including films, hydrogels, and aerogels, with improved mechanical performance and antibacterial activity.
33253535	5	21	theme	CNC-protein-based	970:986	arg1	materials					988:996	other CNC-protein-based materials	964:996	other CNC-protein-based materials	964:996	We anticipate that the results presented herein could also contribute to the preparation of other CNC-protein-based materials, including films, hydrogels, and aerogels, with improved mechanical performance and antibacterial activity.
33253535	5	21	theme	CNC-protein-based	970:986	arg1	films					1009:1013	films	1009:1013	films	1009:1013	We anticipate that the results presented herein could also contribute to the preparation of other CNC-protein-based materials, including films, hydrogels, and aerogels, with improved mechanical performance and antibacterial activity.
33253535	5	21	theme	CNC-protein-based	970:986	arg1	aerogels					1031:1038	aerogels	1031:1038	aerogels	1031:1038	We anticipate that the results presented herein could also contribute to the preparation of other CNC-protein-based materials, including films, hydrogels, and aerogels, with improved mechanical performance and antibacterial activity.
33253535	2	22	theme	films	305:309	arg1	preparation					243:253	the preparation	239:253	the preparation of cellulose nanocrystal (CNC)-lysozyme composite films	239:309	Here, we investigate the preparation of cellulose nanocrystal (CNC)-lysozyme composite films via the well-established method of evaporation-induced self-assembly.
33253535	5	23	theme	mechanical	1055:1064	arg1	performance					1066:1076	improved mechanical performance	1046:1076	improved mechanical performance	1046:1076	We anticipate that the results presented herein could also contribute to the preparation of other CNC-protein-based materials, including films, hydrogels, and aerogels, with improved mechanical performance and antibacterial activity.
33253535	1	24	theme	antibacterial	193:205	arg1	materials					207:215	reliable, sustainable, and cost-effective antibacterial materials	151:215	reliable, sustainable, and cost-effective antibacterial materials	151:215	In modern society, there is a constant need for developing reliable, sustainable, and cost-effective antibacterial materials.
33253535	0	25	theme	Cellulose	12:20	arg1	Films					53:57	Cellulose Nanocrystal-Lysozyme Composite Films	12:57	Cellulose Nanocrystal-Lysozyme Composite Films	12:57	Assembly of Cellulose Nanocrystal-Lysozyme Composite Films with Varied Lysozyme Morphology.
33253535	4	26	theme	higher	599:604	arg1	levels					623:628	higher lysozyme loading levels	599:628	higher lysozyme loading levels (ca. 10 wt %)	599:642	Although at higher lysozyme loading levels (ca. 10 wt %), composite films lost their characteristic chiral nematic structuring, these films demonstrated improved mechanical properties and antibacterial activity with respect to CNC-only films, regardless of lysozyme aggregation state.
33253535	4	26	theme	higher	599:604	arg1	%					641:641	10 wt %	635:641	10 wt %	635:641	Although at higher lysozyme loading levels (ca. 10 wt %), composite films lost their characteristic chiral nematic structuring, these films demonstrated improved mechanical properties and antibacterial activity with respect to CNC-only films, regardless of lysozyme aggregation state.
33253535	2	27	theme	composite	295:303	arg1	films					305:309	cellulose nanocrystal (CNC)-lysozyme composite films	258:309	cellulose nanocrystal (CNC)-lysozyme composite films	258:309	Here, we investigate the preparation of cellulose nanocrystal (CNC)-lysozyme composite films via the well-established method of evaporation-induced self-assembly.
33253535	3	28	theme	aggregation	435:445	arg1	state					447:451	aggregation state	435:451	aggregation state	435:451	We consider the effects of lysozyme concentration and aggregation state (native lysozyme, lysozyme amyloid fibers, and sonicated lysozyme amyloid fibers) on suspension aggregation and film-forming ability.
33253535	4	29	theme	aggregation	853:863	arg1	state					865:869	lysozyme aggregation state	844:869	lysozyme aggregation state	844:869	Although at higher lysozyme loading levels (ca. 10 wt %), composite films lost their characteristic chiral nematic structuring, these films demonstrated improved mechanical properties and antibacterial activity with respect to CNC-only films, regardless of lysozyme aggregation state.
33253535	3	30	theme	native	454:459	arg1	lysozyme					461:468	native lysozyme	454:468	native lysozyme	454:468	We consider the effects of lysozyme concentration and aggregation state (native lysozyme, lysozyme amyloid fibers, and sonicated lysozyme amyloid fibers) on suspension aggregation and film-forming ability.
33253535	0	31	theme	Composite	43:51	arg1	Films					53:57	Cellulose Nanocrystal-Lysozyme Composite Films	12:57	Cellulose Nanocrystal-Lysozyme Composite Films	12:57	Assembly of Cellulose Nanocrystal-Lysozyme Composite Films with Varied Lysozyme Morphology.
33253535	3	32	theme	state	447:451	arg1	effects					397:403	the effects	393:403	the effects of lysozyme concentration and aggregation state (native lysozyme, lysozyme amyloid fibers, and sonicated lysozyme amyloid fibers) on suspension aggregation and film-forming ability	393:584	We consider the effects of lysozyme concentration and aggregation state (native lysozyme, lysozyme amyloid fibers, and sonicated lysozyme amyloid fibers) on suspension aggregation and film-forming ability.
33253535	4	33	theme	lysozyme	844:851	arg1	state					865:869	lysozyme aggregation state	844:869	lysozyme aggregation state	844:869	Although at higher lysozyme loading levels (ca. 10 wt %), composite films lost their characteristic chiral nematic structuring, these films demonstrated improved mechanical properties and antibacterial activity with respect to CNC-only films, regardless of lysozyme aggregation state.
33253535	3	34	theme	amyloid	480:486	arg1	fibers					488:493	amyloid fibers	480:493	lysozyme amyloid fibers	471:493	We consider the effects of lysozyme concentration and aggregation state (native lysozyme, lysozyme amyloid fibers, and sonicated lysozyme amyloid fibers) on suspension aggregation and film-forming ability.
33253535	3	35	theme	concentration	417:429	arg1	effects					397:403	the effects	393:403	the effects of lysozyme concentration and aggregation state (native lysozyme, lysozyme amyloid fibers, and sonicated lysozyme amyloid fibers) on suspension aggregation and film-forming ability	393:584	We consider the effects of lysozyme concentration and aggregation state (native lysozyme, lysozyme amyloid fibers, and sonicated lysozyme amyloid fibers) on suspension aggregation and film-forming ability.
33253535	0	36	theme	Lysozyme	71:78	arg1	Morphology					80:89	Varied Lysozyme Morphology	64:89	Varied Lysozyme Morphology	64:89	Assembly of Cellulose Nanocrystal-Lysozyme Composite Films with Varied Lysozyme Morphology.
33253535	3	37	dep	lysozyme	461:468	arg1	fibers					527:532	amyloid fibers	519:532	amyloid fibers	519:532	We consider the effects of lysozyme concentration and aggregation state (native lysozyme, lysozyme amyloid fibers, and sonicated lysozyme amyloid fibers) on suspension aggregation and film-forming ability.
33253535	2	38	theme	evaporation-induced	346:364	arg1	self-assembly					366:378	evaporation-induced self-assembly	346:378	evaporation-induced self-assembly	346:378	Here, we investigate the preparation of cellulose nanocrystal (CNC)-lysozyme composite films via the well-established method of evaporation-induced self-assembly.
33253535	4	39	dep	%	641:641	arg1	ca.					631:633	ca.	631:633	ca.	631:633	Although at higher lysozyme loading levels (ca. 10 wt %), composite films lost their characteristic chiral nematic structuring, these films demonstrated improved mechanical properties and antibacterial activity with respect to CNC-only films, regardless of lysozyme aggregation state.
33253535	5	40	theme	antibacterial	1082:1094	arg1	activity					1096:1103	antibacterial activity	1082:1103	antibacterial activity	1082:1103	We anticipate that the results presented herein could also contribute to the preparation of other CNC-protein-based materials, including films, hydrogels, and aerogels, with improved mechanical performance and antibacterial activity.
33253535	3	41	theme	amyloid	519:525	arg1	fibers					527:532	amyloid fibers	519:532	amyloid fibers	519:532	We consider the effects of lysozyme concentration and aggregation state (native lysozyme, lysozyme amyloid fibers, and sonicated lysozyme amyloid fibers) on suspension aggregation and film-forming ability.
33253535	4	42	theme	characteristic	672:685	arg1	structuring					702:712	their characteristic chiral nematic structuring	666:712	their characteristic chiral nematic structuring	666:712	Although at higher lysozyme loading levels (ca. 10 wt %), composite films lost their characteristic chiral nematic structuring, these films demonstrated improved mechanical properties and antibacterial activity with respect to CNC-only films, regardless of lysozyme aggregation state.
33253535	3	43	dep	lysozyme	471:478	arg1	fibers					488:493	amyloid fibers	480:493	lysozyme amyloid fibers	471:493	We consider the effects of lysozyme concentration and aggregation state (native lysozyme, lysozyme amyloid fibers, and sonicated lysozyme amyloid fibers) on suspension aggregation and film-forming ability.
33253535	0	44	with	Assembly	0:7	arg1	Morphology					80:89	Varied Lysozyme Morphology	64:89	Varied Lysozyme Morphology	64:89	Assembly of Cellulose Nanocrystal-Lysozyme Composite Films with Varied Lysozyme Morphology.
33253535	1	45	theme	constant	122:129	arg1	need					131:134	a constant need	120:134	a constant need for developing reliable, sustainable, and cost-effective antibacterial materials	120:215	In modern society, there is a constant need for developing reliable, sustainable, and cost-effective antibacterial materials.
33253535	3	46	theme	film-forming	565:576	arg1	ability					578:584	film-forming ability	565:584	film-forming ability	565:584	We consider the effects of lysozyme concentration and aggregation state (native lysozyme, lysozyme amyloid fibers, and sonicated lysozyme amyloid fibers) on suspension aggregation and film-forming ability.
33253535	2	47	theme	well-established	319:334	arg1	method					336:341	the well-established method	315:341	the well-established method of evaporation-induced self-assembly	315:378	Here, we investigate the preparation of cellulose nanocrystal (CNC)-lysozyme composite films via the well-established method of evaporation-induced self-assembly.
33253535	4	48	theme	mechanical	749:758	arg1	properties					760:769	improved mechanical properties	740:769	improved mechanical properties	740:769	Although at higher lysozyme loading levels (ca. 10 wt %), composite films lost their characteristic chiral nematic structuring, these films demonstrated improved mechanical properties and antibacterial activity with respect to CNC-only films, regardless of lysozyme aggregation state.
33253535	4	49	theme	composite	645:653	arg1	films					655:659	composite films	645:659	composite films	645:659	Although at higher lysozyme loading levels (ca. 10 wt %), composite films lost their characteristic chiral nematic structuring, these films demonstrated improved mechanical properties and antibacterial activity with respect to CNC-only films, regardless of lysozyme aggregation state.
33253535	0	50	theme	Varied	64:69	arg1	Morphology					80:89	Varied Lysozyme Morphology	64:89	Varied Lysozyme Morphology	64:89	Assembly of Cellulose Nanocrystal-Lysozyme Composite Films with Varied Lysozyme Morphology.
33253535	5	51	theme	improved	1046:1053	arg1	performance					1066:1076	improved mechanical performance	1046:1076	improved mechanical performance	1046:1076	We anticipate that the results presented herein could also contribute to the preparation of other CNC-protein-based materials, including films, hydrogels, and aerogels, with improved mechanical performance and antibacterial activity.
33253535	3	52	theme	suspension	538:547	arg1	aggregation					549:559	suspension aggregation	538:559	suspension aggregation	538:559	We consider the effects of lysozyme concentration and aggregation state (native lysozyme, lysozyme amyloid fibers, and sonicated lysozyme amyloid fibers) on suspension aggregation and film-forming ability.
33253535	4	53	theme	wt	638:639	arg1	levels					623:628	higher lysozyme loading levels	599:628	higher lysozyme loading levels (ca. 10 wt %)	599:642	Although at higher lysozyme loading levels (ca. 10 wt %), composite films lost their characteristic chiral nematic structuring, these films demonstrated improved mechanical properties and antibacterial activity with respect to CNC-only films, regardless of lysozyme aggregation state.
33253535	4	53	theme	wt	638:639	arg1	%					641:641	10 wt %	635:641	10 wt %	635:641	Although at higher lysozyme loading levels (ca. 10 wt %), composite films lost their characteristic chiral nematic structuring, these films demonstrated improved mechanical properties and antibacterial activity with respect to CNC-only films, regardless of lysozyme aggregation state.
33838822	2	0	theme	composition	427:437	arg1	analysis					439:446	monosaccharide composition analysis	412:446	monosaccharide composition analysis	412:446	By combining monosaccharide composition analysis, methylation analysis and 1D & 2D NMR, the structure of sub-fractions was systematically characterized.
33838822	3	1	theme	1.5:1.6:1.0	644:654	arg1	ratio					635:639	the molar ratio	625:639	the molar ratio of 1.5:1.6:1.0 and 10.6:1.0:13.8, respectively	625:686	Both MPS-1 and MPS-2 were comprised of mannose, glucose and galactose in the molar ratio of 1.5:1.6:1.0 and 10.6:1.0:13.8, respectively.
33838822	6	2	theme	MPS-4	925:929	arg1	structures					901:910	The proposed structures	888:910	The proposed structures of MPS-3 and MPS-4	888:929	The proposed structures of MPS-3 and MPS-4 were not obtained due to the fairly complex molecular structure and relatively low yield.
33838822	2	3	theme	monosaccharide	412:425	arg1	analysis					439:446	monosaccharide composition analysis	412:446	monosaccharide composition analysis	412:446	By combining monosaccharide composition analysis, methylation analysis and 1D & 2D NMR, the structure of sub-fractions was systematically characterized.
33838822	0	4	from	characterization	11:26	arg1	fermentation					97:108	liquid fermentation	90:108	liquid fermentation of Monascus purpureus (Hong Qu)	90:140	Structural characterization and immunomodulatory activity of mycelium polysaccharide from liquid fermentation of Monascus purpureus (Hong Qu).
33838822	6	5	theme	low	1010:1012	arg1	yield					1014:1018	relatively low yield	999:1018	relatively low yield	999:1018	The proposed structures of MPS-3 and MPS-4 were not obtained due to the fairly complex molecular structure and relatively low yield.
33838822	0	6	theme	purpureus	122:130	arg1	fermentation					97:108	liquid fermentation	90:108	liquid fermentation of Monascus purpureus (Hong Qu)	90:140	Structural characterization and immunomodulatory activity of mycelium polysaccharide from liquid fermentation of Monascus purpureus (Hong Qu).
33838822	6	7	theme	MPS-3	915:919	arg1	structures					901:910	The proposed structures	888:910	The proposed structures of MPS-3 and MPS-4	888:929	The proposed structures of MPS-3 and MPS-4 were not obtained due to the fairly complex molecular structure and relatively low yield.
33838822	0	8	theme	Monascus	113:120	arg1	purpureus					122:130	Monascus purpureus	113:130	Monascus purpureus (Hong Qu)	113:140	Structural characterization and immunomodulatory activity of mycelium polysaccharide from liquid fermentation of Monascus purpureus (Hong Qu).
33838822	0	8	theme	Monascus	113:120	arg1	Qu					138:139	Qu	138:139	Qu	138:139	Structural characterization and immunomodulatory activity of mycelium polysaccharide from liquid fermentation of Monascus purpureus (Hong Qu).
33838822	1	9	theme	DEAE-Cellulose	361:374	arg1	chromatography					383:396	DEAE-Cellulose column chromatography	361:396	DEAE-Cellulose column chromatography	361:396	Alkaline extracted endopolysaccharides (MPS) from Monascus purpureus (Hong Qu) mycelium were successfully separated into four sub-fractions, namely MPS-1 (18.0 %), MPS-2 (27.1 %), MPS-3 (12.6 %) and MPS-4 (14.7 %), by DEAE-Cellulose column chromatography.
33838822	3	10	theme	10.6:1.0:13.8	660:672	arg1	ratio					635:639	the molar ratio	625:639	the molar ratio of 1.5:1.6:1.0 and 10.6:1.0:13.8, respectively	625:686	Both MPS-1 and MPS-2 were comprised of mannose, glucose and galactose in the molar ratio of 1.5:1.6:1.0 and 10.6:1.0:13.8, respectively.
33838822	1	11	theme	column	376:381	arg1	chromatography					383:396	DEAE-Cellulose column chromatography	361:396	DEAE-Cellulose column chromatography	361:396	Alkaline extracted endopolysaccharides (MPS) from Monascus purpureus (Hong Qu) mycelium were successfully separated into four sub-fractions, namely MPS-1 (18.0 %), MPS-2 (27.1 %), MPS-3 (12.6 %) and MPS-4 (14.7 %), by DEAE-Cellulose column chromatography.
33838822	2	12	theme	methylation	449:459	arg1	analysis					461:468	methylation analysis	449:468	methylation analysis	449:468	By combining monosaccharide composition analysis, methylation analysis and 1D & 2D NMR, the structure of sub-fractions was systematically characterized.
33838822	4	13	theme	them	705:708	arg1	backbone					693:700	The backbone	689:700	The backbone of them both	689:713	The backbone of them both consisted of 2-α-Manp with several different branched chains.
33838822	5	14	theme	glucose	802:808	arg1	4-Glcp					850:855	4-Glcp	850:855	4-Glcp	850:855	However, MPS-1 contained glucose based sugar residues such as 3-Glcp and 4-Glcp which were not shown on MPS-2.
33838822	5	14	theme	glucose	802:808	arg1	residues					822:829	glucose based sugar residues	802:829	glucose based sugar residues such as 3-Glcp and 4-Glcp which were not shown on MPS-2	802:885	However, MPS-1 contained glucose based sugar residues such as 3-Glcp and 4-Glcp which were not shown on MPS-2.
33838822	5	14	theme	glucose	802:808	arg1	3-Glcp					839:844	3-Glcp	839:844	3-Glcp	839:844	However, MPS-1 contained glucose based sugar residues such as 3-Glcp and 4-Glcp which were not shown on MPS-2.
33838822	0	15	from	activity	49:56	arg1	fermentation					97:108	liquid fermentation	90:108	liquid fermentation of Monascus purpureus (Hong Qu)	90:140	Structural characterization and immunomodulatory activity of mycelium polysaccharide from liquid fermentation of Monascus purpureus (Hong Qu).
33838822	5	16	theme	based	810:814	arg1	4-Glcp					850:855	4-Glcp	850:855	4-Glcp	850:855	However, MPS-1 contained glucose based sugar residues such as 3-Glcp and 4-Glcp which were not shown on MPS-2.
33838822	5	16	theme	based	810:814	arg1	residues					822:829	glucose based sugar residues	802:829	glucose based sugar residues such as 3-Glcp and 4-Glcp which were not shown on MPS-2	802:885	However, MPS-1 contained glucose based sugar residues such as 3-Glcp and 4-Glcp which were not shown on MPS-2.
33838822	5	16	theme	based	810:814	arg1	3-Glcp					839:844	3-Glcp	839:844	3-Glcp	839:844	However, MPS-1 contained glucose based sugar residues such as 3-Glcp and 4-Glcp which were not shown on MPS-2.
33838822	0	17	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunomodulatory activity of mycelium polysaccharide from liquid fermentation of Monascus purpureus (Hong Qu).
33838822	1	18	theme	purpureus	202:210	arg1	mycelium					222:229	Monascus purpureus (Hong Qu) mycelium	193:229	Monascus purpureus (Hong Qu) mycelium	193:229	Alkaline extracted endopolysaccharides (MPS) from Monascus purpureus (Hong Qu) mycelium were successfully separated into four sub-fractions, namely MPS-1 (18.0 %), MPS-2 (27.1 %), MPS-3 (12.6 %) and MPS-4 (14.7 %), by DEAE-Cellulose column chromatography.
33838822	0	19	theme	immunomodulatory	32:47	arg1	activity					49:56	immunomodulatory activity	32:56	immunomodulatory activity	32:56	Structural characterization and immunomodulatory activity of mycelium polysaccharide from liquid fermentation of Monascus purpureus (Hong Qu).
33838822	6	20	theme	proposed	892:899	arg1	structures					901:910	The proposed structures	888:910	The proposed structures of MPS-3 and MPS-4	888:929	The proposed structures of MPS-3 and MPS-4 were not obtained due to the fairly complex molecular structure and relatively low yield.
33838822	5	21	contain	contained	792:800	arg2	residues					822:829	glucose based sugar residues	802:829	glucose based sugar residues such as 3-Glcp and 4-Glcp which were not shown on MPS-2	802:885	However, MPS-1 contained glucose based sugar residues such as 3-Glcp and 4-Glcp which were not shown on MPS-2.
33838822	5	21	contain	contained	792:800	arg2	4-Glcp					850:855	4-Glcp	850:855	4-Glcp	850:855	However, MPS-1 contained glucose based sugar residues such as 3-Glcp and 4-Glcp which were not shown on MPS-2.
33838822	5	21	contain	contained	792:800	arg1	MPS-1					786:790	MPS-1	786:790	MPS-1	786:790	However, MPS-1 contained glucose based sugar residues such as 3-Glcp and 4-Glcp which were not shown on MPS-2.
33838822	5	21	contain	contained	792:800	arg2	3-Glcp					839:844	3-Glcp	839:844	3-Glcp	839:844	However, MPS-1 contained glucose based sugar residues such as 3-Glcp and 4-Glcp which were not shown on MPS-2.
33838822	6	22	theme	complex	967:973	arg1	structure					985:993	the fairly complex molecular structure	956:993	the fairly complex molecular structure	956:993	The proposed structures of MPS-3 and MPS-4 were not obtained due to the fairly complex molecular structure and relatively low yield.
33838822	2	23	theme	2D	479:480	arg1	NMR					482:484	1D & 2D NMR	474:484	1D & 2D NMR	474:484	By combining monosaccharide composition analysis, methylation analysis and 1D & 2D NMR, the structure of sub-fractions was systematically characterized.
33838822	0	24	from	fermentation	97:108	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunomodulatory activity of mycelium polysaccharide from liquid fermentation of Monascus purpureus (Hong Qu).
33838822	0	24	from	fermentation	97:108	arg1	activity					49:56	immunomodulatory activity	32:56	immunomodulatory activity	32:56	Structural characterization and immunomodulatory activity of mycelium polysaccharide from liquid fermentation of Monascus purpureus (Hong Qu).
33838822	0	24	from	fermentation	97:108	arg1	polysaccharide					70:83	mycelium polysaccharide	61:83	mycelium polysaccharide from liquid fermentation of Monascus purpureus (Hong Qu)	61:140	Structural characterization and immunomodulatory activity of mycelium polysaccharide from liquid fermentation of Monascus purpureus (Hong Qu).
33838822	4	25	theme	different	750:758	arg1	chains					769:774	several different branched chains	742:774	several different branched chains	742:774	The backbone of them both consisted of 2-α-Manp with several different branched chains.
33838822	5	26	theme	sugar	816:820	arg1	4-Glcp					850:855	4-Glcp	850:855	4-Glcp	850:855	However, MPS-1 contained glucose based sugar residues such as 3-Glcp and 4-Glcp which were not shown on MPS-2.
33838822	5	26	theme	sugar	816:820	arg1	residues					822:829	glucose based sugar residues	802:829	glucose based sugar residues such as 3-Glcp and 4-Glcp which were not shown on MPS-2	802:885	However, MPS-1 contained glucose based sugar residues such as 3-Glcp and 4-Glcp which were not shown on MPS-2.
33838822	5	26	theme	sugar	816:820	arg1	3-Glcp					839:844	3-Glcp	839:844	3-Glcp	839:844	However, MPS-1 contained glucose based sugar residues such as 3-Glcp and 4-Glcp which were not shown on MPS-2.
33838822	7	27	theme	related	1191:1197	arg1	mRNA					1199:1202	the related mRNA	1187:1202	the related mRNA	1187:1202	Moreover, based on the RAW 264.7 cells model, MPS-2 could significantly promote cytokines secretion including IL-6, TNF-α, and IL-10 and improve expression levels of the related mRNA.
33838822	7	28	theme	model	1060:1064	arg1	cells					1054:1058	the RAW 264.7 cells model	1040:1064	the RAW 264.7 cells model	1040:1064	Moreover, based on the RAW 264.7 cells model, MPS-2 could significantly promote cytokines secretion including IL-6, TNF-α, and IL-10 and improve expression levels of the related mRNA.
33838822	2	29	theme	&	477:477	arg1	NMR					482:484	1D & 2D NMR	474:484	1D & 2D NMR	474:484	By combining monosaccharide composition analysis, methylation analysis and 1D & 2D NMR, the structure of sub-fractions was systematically characterized.
33838822	4	30	theme	several	742:748	arg1	chains					769:774	several different branched chains	742:774	several different branched chains	742:774	The backbone of them both consisted of 2-α-Manp with several different branched chains.
33838822	7	31	theme	mRNA	1199:1202	arg1	levels					1177:1182	expression levels	1166:1182	expression levels of the related mRNA	1166:1202	Moreover, based on the RAW 264.7 cells model, MPS-2 could significantly promote cytokines secretion including IL-6, TNF-α, and IL-10 and improve expression levels of the related mRNA.
33838822	0	32	theme	polysaccharide	70:83	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunomodulatory activity of mycelium polysaccharide from liquid fermentation of Monascus purpureus (Hong Qu).
33838822	0	32	theme	polysaccharide	70:83	arg1	activity					49:56	immunomodulatory activity	32:56	immunomodulatory activity	32:56	Structural characterization and immunomodulatory activity of mycelium polysaccharide from liquid fermentation of Monascus purpureus (Hong Qu).
33838822	2	33	theme	1D	474:475	arg1	NMR					482:484	1D & 2D NMR	474:484	1D & 2D NMR	474:484	By combining monosaccharide composition analysis, methylation analysis and 1D & 2D NMR, the structure of sub-fractions was systematically characterized.
33838822	2	34	theme	sub-fractions	504:516	arg1	structure					491:499	the structure	487:499	the structure of sub-fractions	487:516	By combining monosaccharide composition analysis, methylation analysis and 1D & 2D NMR, the structure of sub-fractions was systematically characterized.
33838822	7	35	dep	secretion	1111:1119	arg1	including					1121:1129	including	1121:1129	including	1121:1129	Moreover, based on the RAW 264.7 cells model, MPS-2 could significantly promote cytokines secretion including IL-6, TNF-α, and IL-10 and improve expression levels of the related mRNA.
33838822	7	35	dep	secretion	1111:1119	arg1	TNF-α					1137:1141	TNF-α	1137:1141	TNF-α	1137:1141	Moreover, based on the RAW 264.7 cells model, MPS-2 could significantly promote cytokines secretion including IL-6, TNF-α, and IL-10 and improve expression levels of the related mRNA.
33838822	7	35	dep	secretion	1111:1119	arg1	IL-10					1148:1152	IL-10	1148:1152	IL-10	1148:1152	Moreover, based on the RAW 264.7 cells model, MPS-2 could significantly promote cytokines secretion including IL-6, TNF-α, and IL-10 and improve expression levels of the related mRNA.
33838822	7	35	dep	secretion	1111:1119	arg1	IL-6					1131:1134	IL-6	1131:1134	IL-6	1131:1134	Moreover, based on the RAW 264.7 cells model, MPS-2 could significantly promote cytokines secretion including IL-6, TNF-α, and IL-10 and improve expression levels of the related mRNA.
33838822	0	36	theme	mycelium	61:68	arg1	polysaccharide					70:83	mycelium polysaccharide	61:83	mycelium polysaccharide from liquid fermentation of Monascus purpureus (Hong Qu)	61:140	Structural characterization and immunomodulatory activity of mycelium polysaccharide from liquid fermentation of Monascus purpureus (Hong Qu).
33838822	4	37	theme	branched	760:767	arg1	chains					769:774	several different branched chains	742:774	several different branched chains	742:774	The backbone of them both consisted of 2-α-Manp with several different branched chains.
33838822	1	38	from	mycelium	222:229	arg1	endopolysaccharides					162:180	Alkaline extracted endopolysaccharides	143:180	Alkaline extracted endopolysaccharides (MPS) from Monascus purpureus (Hong Qu) mycelium	143:229	Alkaline extracted endopolysaccharides (MPS) from Monascus purpureus (Hong Qu) mycelium were successfully separated into four sub-fractions, namely MPS-1 (18.0 %), MPS-2 (27.1 %), MPS-3 (12.6 %) and MPS-4 (14.7 %), by DEAE-Cellulose column chromatography.
33838822	1	38	from	mycelium	222:229	arg1	MPS					183:185	MPS	183:185	MPS	183:185	Alkaline extracted endopolysaccharides (MPS) from Monascus purpureus (Hong Qu) mycelium were successfully separated into four sub-fractions, namely MPS-1 (18.0 %), MPS-2 (27.1 %), MPS-3 (12.6 %) and MPS-4 (14.7 %), by DEAE-Cellulose column chromatography.
33838822	7	39	theme	expression	1166:1175	arg1	levels					1177:1182	expression levels	1166:1182	expression levels of the related mRNA	1166:1202	Moreover, based on the RAW 264.7 cells model, MPS-2 could significantly promote cytokines secretion including IL-6, TNF-α, and IL-10 and improve expression levels of the related mRNA.
33838822	6	40	theme	molecular	975:983	arg1	structure					985:993	the fairly complex molecular structure	956:993	the fairly complex molecular structure	956:993	The proposed structures of MPS-3 and MPS-4 were not obtained due to the fairly complex molecular structure and relatively low yield.
33838822	1	41	theme	Alkaline	143:150	arg1	endopolysaccharides					162:180	Alkaline extracted endopolysaccharides	143:180	Alkaline extracted endopolysaccharides (MPS) from Monascus purpureus (Hong Qu) mycelium	143:229	Alkaline extracted endopolysaccharides (MPS) from Monascus purpureus (Hong Qu) mycelium were successfully separated into four sub-fractions, namely MPS-1 (18.0 %), MPS-2 (27.1 %), MPS-3 (12.6 %) and MPS-4 (14.7 %), by DEAE-Cellulose column chromatography.
33838822	1	41	theme	Alkaline	143:150	arg1	MPS					183:185	MPS	183:185	MPS	183:185	Alkaline extracted endopolysaccharides (MPS) from Monascus purpureus (Hong Qu) mycelium were successfully separated into four sub-fractions, namely MPS-1 (18.0 %), MPS-2 (27.1 %), MPS-3 (12.6 %) and MPS-4 (14.7 %), by DEAE-Cellulose column chromatography.
33838822	0	42	theme	liquid	90:95	arg1	fermentation					97:108	liquid fermentation	90:108	liquid fermentation of Monascus purpureus (Hong Qu)	90:140	Structural characterization and immunomodulatory activity of mycelium polysaccharide from liquid fermentation of Monascus purpureus (Hong Qu).
33838822	1	43	theme	extracted	152:160	arg1	endopolysaccharides					162:180	Alkaline extracted endopolysaccharides	143:180	Alkaline extracted endopolysaccharides (MPS) from Monascus purpureus (Hong Qu) mycelium	143:229	Alkaline extracted endopolysaccharides (MPS) from Monascus purpureus (Hong Qu) mycelium were successfully separated into four sub-fractions, namely MPS-1 (18.0 %), MPS-2 (27.1 %), MPS-3 (12.6 %) and MPS-4 (14.7 %), by DEAE-Cellulose column chromatography.
33838822	1	43	theme	extracted	152:160	arg1	MPS					183:185	MPS	183:185	MPS	183:185	Alkaline extracted endopolysaccharides (MPS) from Monascus purpureus (Hong Qu) mycelium were successfully separated into four sub-fractions, namely MPS-1 (18.0 %), MPS-2 (27.1 %), MPS-3 (12.6 %) and MPS-4 (14.7 %), by DEAE-Cellulose column chromatography.
33838822	7	44	theme	RAW	1044:1046	arg1	cells					1054:1058	the RAW 264.7 cells model	1040:1064	the RAW 264.7 cells model	1040:1064	Moreover, based on the RAW 264.7 cells model, MPS-2 could significantly promote cytokines secretion including IL-6, TNF-α, and IL-10 and improve expression levels of the related mRNA.
33838822	3	45	theme	molar	629:633	arg1	ratio					635:639	the molar ratio	625:639	the molar ratio of 1.5:1.6:1.0 and 10.6:1.0:13.8, respectively	625:686	Both MPS-1 and MPS-2 were comprised of mannose, glucose and galactose in the molar ratio of 1.5:1.6:1.0 and 10.6:1.0:13.8, respectively.
32466232	5	0	theme	thermal	744:750	arg1	treatment					752:760	thermal treatment	744:760	thermal treatment	744:760	After thermal treatment and freeze-drying, the resulting product showed condensation and cross-linking, which was studied with infrared spectroscopy and 13C and 29Si solid-state nuclear magnetic resonance (NMR) spectroscopy.
32466232	3	1	theme	cellulose	484:492	arg1	DAC					529:531	DAC	529:531	DAC	529:531	Silanization has been widely used for cellulosic and nano-structured celluloses, but was a problem so far if to be applied to the common cellulose derivative "dialdehyde cellulose" (DAC), i.e., highly periodate-oxidized celluloses.
32466232	3	1	theme	cellulose	484:492	arg1	"					526:526	the common cellulose derivative "dialdehyde cellulose"	473:526	the common cellulose derivative "dialdehyde cellulose" (DAC)	473:532	Silanization has been widely used for cellulosic and nano-structured celluloses, but was a problem so far if to be applied to the common cellulose derivative "dialdehyde cellulose" (DAC), i.e., highly periodate-oxidized celluloses.
32466232	5	2	theme	13C	891:893	arg1	spectroscopy					949:960	13C and 29Si solid-state nuclear magnetic resonance (NMR) spectroscopy	891:960	spectroscopy	949:960	After thermal treatment and freeze-drying, the resulting product showed condensation and cross-linking, which was studied with infrared spectroscopy and 13C and 29Si solid-state nuclear magnetic resonance (NMR) spectroscopy.
32466232	3	3	theme	derivative	494:503	arg1	DAC					529:531	DAC	529:531	DAC	529:531	Silanization has been widely used for cellulosic and nano-structured celluloses, but was a problem so far if to be applied to the common cellulose derivative "dialdehyde cellulose" (DAC), i.e., highly periodate-oxidized celluloses.
32466232	3	3	theme	derivative	494:503	arg1	"					526:526	the common cellulose derivative "dialdehyde cellulose"	473:526	the common cellulose derivative "dialdehyde cellulose" (DAC)	473:532	Silanization has been widely used for cellulosic and nano-structured celluloses, but was a problem so far if to be applied to the common cellulose derivative "dialdehyde cellulose" (DAC), i.e., highly periodate-oxidized celluloses.
32466232	5	4	theme	resonance	933:941	arg1	spectroscopy					949:960	13C and 29Si solid-state nuclear magnetic resonance (NMR) spectroscopy	891:960	spectroscopy	949:960	After thermal treatment and freeze-drying, the resulting product showed condensation and cross-linking, which was studied with infrared spectroscopy and 13C and 29Si solid-state nuclear magnetic resonance (NMR) spectroscopy.
32466232	8	5	theme	modified	1394:1401	arg1	structure					1424:1432	the modified dialdehyde cellulose structure	1390:1432	the modified dialdehyde cellulose structure	1390:1432	Different treatments cause different levels of hydrolysis of the organosilane compound, which resulted in diverse condensed silica networks in the modified dialdehyde cellulose structure.
32466232	2	6	theme	routes	277:282	arg1	routes					277:282	the most promising routes	258:282	the most promising routes	258:282	Silanization in aqueous media is one of the most promising routes to create multipurpose and organic-inorganic hybrid materials.
32466232	2	6	theme	routes	277:282	arg1	one					251:253	one	251:253	one	251:253	Silanization in aqueous media is one of the most promising routes to create multipurpose and organic-inorganic hybrid materials.
32466232	8	7	theme	diverse	1353:1359	arg1	networks					1378:1385	diverse condensed silica networks	1353:1385	diverse condensed silica networks in the modified dialdehyde cellulose structure	1353:1432	Different treatments cause different levels of hydrolysis of the organosilane compound, which resulted in diverse condensed silica networks in the modified dialdehyde cellulose structure.
32466232	4	8	theme	3-aminopropyl	707:719	arg1	triethoxysilane					721:735	(3-aminopropyl)triethoxysilane	706:735	(3-aminopropyl)triethoxysilane	706:735	In this work, a straightforward silanization protocol for dialdehyde cellulose is proposed, which can be readily modified with (3-aminopropyl)triethoxysilane.
32466232	6	9	theme	keto	1131:1134	arg1	functions					1136:1144	keto functions	1131:1144	keto functions of the DAC (-HC=N-)	1131:1164	The cross-linking involves both links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C) and imine-type bonds between the amino group and keto functions of the DAC (-HC=N-).
32466232	6	10	theme	DAC	1153:1155	arg1	group					1121:1125	the amino group	1111:1125	the amino group	1111:1125	The cross-linking involves both links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C) and imine-type bonds between the amino group and keto functions of the DAC (-HC=N-).
32466232	6	10	theme	DAC	1153:1155	arg1	functions					1136:1144	keto functions	1131:1144	keto functions of the DAC (-HC=N-)	1131:1164	The cross-linking involves both links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C) and imine-type bonds between the amino group and keto functions of the DAC (-HC=N-).
32466232	6	10	theme	DAC	1153:1155	arg1	DAC					1153:1155	the DAC	1149:1155	the DAC (-HC=N-)	1149:1164	The cross-linking involves both links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C) and imine-type bonds between the amino group and keto functions of the DAC (-HC=N-).
32466232	6	10	theme	DAC	1153:1155	arg1	-HC=N-					1158:1163	-HC=N-	1158:1163	-HC=N-	1158:1163	The cross-linking involves both links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C) and imine-type bonds between the amino group and keto functions of the DAC (-HC=N-).
32466232	2	11	theme	promising	267:275	arg1	routes					277:282	the most promising routes	258:282	the most promising routes	258:282	Silanization in aqueous media is one of the most promising routes to create multipurpose and organic-inorganic hybrid materials.
32466232	2	12	from	Silanization	218:229	arg1	media					242:246	aqueous media	234:246	aqueous media	234:246	Silanization in aqueous media is one of the most promising routes to create multipurpose and organic-inorganic hybrid materials.
32466232	5	13	theme	magnetic	924:931	arg1	NMR					944:946	NMR	944:946	NMR	944:946	After thermal treatment and freeze-drying, the resulting product showed condensation and cross-linking, which was studied with infrared spectroscopy and 13C and 29Si solid-state nuclear magnetic resonance (NMR) spectroscopy.
32466232	5	13	theme	magnetic	924:931	arg1	resonance					933:941	nuclear magnetic resonance	916:941	nuclear magnetic resonance (NMR)	916:947	After thermal treatment and freeze-drying, the resulting product showed condensation and cross-linking, which was studied with infrared spectroscopy and 13C and 29Si solid-state nuclear magnetic resonance (NMR) spectroscopy.
32466232	6	14	theme	amino	1115:1119	arg1	group					1121:1125	the amino group	1111:1125	the amino group	1111:1125	The cross-linking involves both links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C) and imine-type bonds between the amino group and keto functions of the DAC (-HC=N-).
32466232	6	14	theme	amino	1115:1119	arg1	DAC					1153:1155	the DAC	1149:1155	the DAC (-HC=N-)	1149:1164	The cross-linking involves both links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C) and imine-type bonds between the amino group and keto functions of the DAC (-HC=N-).
32466232	6	14	theme	amino	1115:1119	arg1	-HC=N-					1158:1163	-HC=N-	1158:1163	-HC=N-	1158:1163	The cross-linking involves both links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C) and imine-type bonds between the amino group and keto functions of the DAC (-HC=N-).
32466232	5	15	theme	resulting	785:793	arg1	product					795:801	the resulting product	781:801	the resulting product	781:801	After thermal treatment and freeze-drying, the resulting product showed condensation and cross-linking, which was studied with infrared spectroscopy and 13C and 29Si solid-state nuclear magnetic resonance (NMR) spectroscopy.
32466232	3	16	theme	cellulose	517:525	arg1	DAC					529:531	DAC	529:531	DAC	529:531	Silanization has been widely used for cellulosic and nano-structured celluloses, but was a problem so far if to be applied to the common cellulose derivative "dialdehyde cellulose" (DAC), i.e., highly periodate-oxidized celluloses.
32466232	3	16	theme	cellulose	517:525	arg1	"					526:526	the common cellulose derivative "dialdehyde cellulose"	473:526	the common cellulose derivative "dialdehyde cellulose" (DAC)	473:532	Silanization has been widely used for cellulosic and nano-structured celluloses, but was a problem so far if to be applied to the common cellulose derivative "dialdehyde cellulose" (DAC), i.e., highly periodate-oxidized celluloses.
32466232	5	17	theme	nuclear	916:922	arg1	NMR					944:946	NMR	944:946	NMR	944:946	After thermal treatment and freeze-drying, the resulting product showed condensation and cross-linking, which was studied with infrared spectroscopy and 13C and 29Si solid-state nuclear magnetic resonance (NMR) spectroscopy.
32466232	5	17	theme	nuclear	916:922	arg1	resonance					933:941	nuclear magnetic resonance	916:941	nuclear magnetic resonance (NMR)	916:947	After thermal treatment and freeze-drying, the resulting product showed condensation and cross-linking, which was studied with infrared spectroscopy and 13C and 29Si solid-state nuclear magnetic resonance (NMR) spectroscopy.
32466232	4	18	theme	dialdehyde	637:646	arg1	cellulose					648:656	dialdehyde cellulose	637:656	dialdehyde cellulose	637:656	In this work, a straightforward silanization protocol for dialdehyde cellulose is proposed, which can be readily modified with (3-aminopropyl)triethoxysilane.
32466232	8	19	theme	hydrolysis	1294:1303	arg1	levels					1284:1289	different levels	1274:1289	different levels	1274:1289	Different treatments cause different levels of hydrolysis of the organosilane compound, which resulted in diverse condensed silica networks in the modified dialdehyde cellulose structure.
32466232	1	20	theme	many	84:87	arg1	applications					99:110	many potential applications	84:110	many potential applications	84:110	Cellulose derivatives have many potential applications in the field of biomaterials and composites, in addition to several ways of modification leading to them.
32466232	8	21	from	networks	1378:1385	arg1	structure					1424:1432	the modified dialdehyde cellulose structure	1390:1432	the modified dialdehyde cellulose structure	1390:1432	Different treatments cause different levels of hydrolysis of the organosilane compound, which resulted in diverse condensed silica networks in the modified dialdehyde cellulose structure.
32466232	1	22	theme	several	172:178	arg1	ways					180:183	several ways	172:183	several ways of modification leading to them	172:215	Cellulose derivatives have many potential applications in the field of biomaterials and composites, in addition to several ways of modification leading to them.
32466232	0	23	theme	Direct	2:7	arg1	Protocol					22:29	A Direct Silanization Protocol	0:29	A Direct Silanization Protocol for Dialdehyde Cellulose	0:54	A Direct Silanization Protocol for Dialdehyde Cellulose.
32466232	1	24	theme	potential	89:97	arg1	applications					99:110	many potential applications	84:110	many potential applications	84:110	Cellulose derivatives have many potential applications in the field of biomaterials and composites, in addition to several ways of modification leading to them.
32466232	3	25	dep	celluloses	567:576	arg1	i.e.					535:538	i.e.	535:538	i.e.	535:538	Silanization has been widely used for cellulosic and nano-structured celluloses, but was a problem so far if to be applied to the common cellulose derivative "dialdehyde cellulose" (DAC), i.e., highly periodate-oxidized celluloses.
32466232	2	26	theme	multipurpose	294:305	arg1	materials					336:344	multipurpose and organic-inorganic hybrid materials	294:344	multipurpose and organic-inorganic hybrid materials	294:344	Silanization in aqueous media is one of the most promising routes to create multipurpose and organic-inorganic hybrid materials.
32466232	5	27	theme	infrared	865:872	arg1	spectroscopy					874:885	infrared spectroscopy	865:885	infrared spectroscopy	865:885	After thermal treatment and freeze-drying, the resulting product showed condensation and cross-linking, which was studied with infrared spectroscopy and 13C and 29Si solid-state nuclear magnetic resonance (NMR) spectroscopy.
32466232	1	28	contain	have	79:82	arg2	applications					99:110	many potential applications	84:110	many potential applications	84:110	Cellulose derivatives have many potential applications in the field of biomaterials and composites, in addition to several ways of modification leading to them.
32466232	1	28	contain	have	79:82	arg1	derivatives					67:77	Cellulose derivatives	57:77	Cellulose derivatives	57:77	Cellulose derivatives have many potential applications in the field of biomaterials and composites, in addition to several ways of modification leading to them.
32466232	8	29	theme	different	1274:1282	arg1	levels					1284:1289	different levels	1274:1289	different levels	1274:1289	Different treatments cause different levels of hydrolysis of the organosilane compound, which resulted in diverse condensed silica networks in the modified dialdehyde cellulose structure.
32466232	6	30	with	group	1017:1021	arg1	Si-O-C					1074:1079	Si-O-C	1074:1079	Si-O-C	1074:1079	The cross-linking involves both links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C) and imine-type bonds between the amino group and keto functions of the DAC (-HC=N-).
32466232	6	30	with	group	1017:1021	arg1	groups					1066:1071	the silanol groups	1054:1071	the silanol groups (Si-O-C)	1054:1080	The cross-linking involves both links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C) and imine-type bonds between the amino group and keto functions of the DAC (-HC=N-).
32466232	8	31	theme	condensed	1361:1369	arg1	networks					1378:1385	diverse condensed silica networks	1353:1385	diverse condensed silica networks in the modified dialdehyde cellulose structure	1353:1432	Different treatments cause different levels of hydrolysis of the organosilane compound, which resulted in diverse condensed silica networks in the modified dialdehyde cellulose structure.
32466232	1	32	dep	ways	180:183	arg1	addition					160:167	addition	160:167	addition	160:167	Cellulose derivatives have many potential applications in the field of biomaterials and composites, in addition to several ways of modification leading to them.
32466232	1	33	theme	modification	188:199	arg1	ways					180:183	several ways	172:183	several ways of modification leading to them	172:215	Cellulose derivatives have many potential applications in the field of biomaterials and composites, in addition to several ways of modification leading to them.
32466232	0	34	theme	Silanization	9:20	arg1	Protocol					22:29	A Direct Silanization Protocol	0:29	A Direct Silanization Protocol for Dialdehyde Cellulose	0:54	A Direct Silanization Protocol for Dialdehyde Cellulose.
32466232	3	35	used	used	376:379	arg2	Silanization					347:358	Silanization	347:358	Silanization	347:358	Silanization has been widely used for cellulosic and nano-structured celluloses, but was a problem so far if to be applied to the common cellulose derivative "dialdehyde cellulose" (DAC), i.e., highly periodate-oxidized celluloses.
32466232	7	36	theme	aqueous	1200:1206	arg1	medium					1208:1213	aqueous medium	1200:1213	aqueous medium	1200:1213	The modification was achieved in aqueous medium under mild reaction conditions.
32466232	6	37	theme	cellulose	1039:1047	arg1	cellulose					1039:1047	the oxidized cellulose	1026:1047	the oxidized cellulose	1026:1047	The cross-linking involves both links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C) and imine-type bonds between the amino group and keto functions of the DAC (-HC=N-).
32466232	6	37	theme	cellulose	1039:1047	arg1	group					1017:1021	the hydroxyl group	1004:1021	the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C)	1004:1080	The cross-linking involves both links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C) and imine-type bonds between the amino group and keto functions of the DAC (-HC=N-).
32466232	0	38	theme	Dialdehyde	35:44	arg1	Cellulose					46:54	Dialdehyde Cellulose	35:54	Dialdehyde Cellulose	35:54	A Direct Silanization Protocol for Dialdehyde Cellulose.
32466232	5	39	theme	solid-state	904:914	arg1	spectroscopy					949:960	13C and 29Si solid-state nuclear magnetic resonance (NMR) spectroscopy	891:960	spectroscopy	949:960	After thermal treatment and freeze-drying, the resulting product showed condensation and cross-linking, which was studied with infrared spectroscopy and 13C and 29Si solid-state nuclear magnetic resonance (NMR) spectroscopy.
32466232	8	40	theme	Different	1247:1255	arg1	treatments					1257:1266	Different treatments	1247:1266	Different treatments	1247:1266	Different treatments cause different levels of hydrolysis of the organosilane compound, which resulted in diverse condensed silica networks in the modified dialdehyde cellulose structure.
32466232	2	41	theme	aqueous	234:240	arg1	media					242:246	aqueous media	234:246	aqueous media	234:246	Silanization in aqueous media is one of the most promising routes to create multipurpose and organic-inorganic hybrid materials.
32466232	6	42	theme	oxidized	1030:1037	arg1	cellulose					1039:1047	the oxidized cellulose	1026:1047	the oxidized cellulose	1026:1047	The cross-linking involves both links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C) and imine-type bonds between the amino group and keto functions of the DAC (-HC=N-).
32466232	6	43	theme	silanol	1058:1064	arg1	Si-O-C					1074:1079	Si-O-C	1074:1079	Si-O-C	1074:1079	The cross-linking involves both links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C) and imine-type bonds between the amino group and keto functions of the DAC (-HC=N-).
32466232	6	43	theme	silanol	1058:1064	arg1	groups					1066:1071	the silanol groups	1054:1071	the silanol groups (Si-O-C)	1054:1080	The cross-linking involves both links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C) and imine-type bonds between the amino group and keto functions of the DAC (-HC=N-).
32466232	8	44	theme	silica	1371:1376	arg1	networks					1378:1385	diverse condensed silica networks	1353:1385	diverse condensed silica networks in the modified dialdehyde cellulose structure	1353:1432	Different treatments cause different levels of hydrolysis of the organosilane compound, which resulted in diverse condensed silica networks in the modified dialdehyde cellulose structure.
32466232	4	45	theme	silanization	611:622	arg1	protocol					624:631	a straightforward silanization protocol	593:631	a straightforward silanization protocol for dialdehyde cellulose	593:656	In this work, a straightforward silanization protocol for dialdehyde cellulose is proposed, which can be readily modified with (3-aminopropyl)triethoxysilane.
32466232	3	46	theme	nano-structured	400:414	arg1	celluloses					416:425	cellulosic and nano-structured celluloses	385:425	cellulosic and nano-structured celluloses	385:425	Silanization has been widely used for cellulosic and nano-structured celluloses, but was a problem so far if to be applied to the common cellulose derivative "dialdehyde cellulose" (DAC), i.e., highly periodate-oxidized celluloses.
32466232	8	47	theme	compound	1325:1332	arg1	hydrolysis					1294:1303	hydrolysis	1294:1303	hydrolysis of the organosilane compound	1294:1332	Different treatments cause different levels of hydrolysis of the organosilane compound, which resulted in diverse condensed silica networks in the modified dialdehyde cellulose structure.
32466232	6	48	theme	imine-type	1086:1095	arg1	bonds					1097:1101	imine-type bonds	1086:1101	imine-type bonds between the amino group and keto functions of the DAC (-HC=N-)	1086:1164	The cross-linking involves both links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C) and imine-type bonds between the amino group and keto functions of the DAC (-HC=N-).
32466232	5	49	theme	29Si	899:902	arg1	spectroscopy					949:960	13C and 29Si solid-state nuclear magnetic resonance (NMR) spectroscopy	891:960	spectroscopy	949:960	After thermal treatment and freeze-drying, the resulting product showed condensation and cross-linking, which was studied with infrared spectroscopy and 13C and 29Si solid-state nuclear magnetic resonance (NMR) spectroscopy.
32466232	3	50	theme	periodate-oxidized	548:565	arg1	celluloses					567:576	highly periodate-oxidized celluloses	541:576	highly periodate-oxidized celluloses	541:576	Silanization has been widely used for cellulosic and nano-structured celluloses, but was a problem so far if to be applied to the common cellulose derivative "dialdehyde cellulose" (DAC), i.e., highly periodate-oxidized celluloses.
32466232	3	51	theme	common	477:482	arg1	DAC					529:531	DAC	529:531	DAC	529:531	Silanization has been widely used for cellulosic and nano-structured celluloses, but was a problem so far if to be applied to the common cellulose derivative "dialdehyde cellulose" (DAC), i.e., highly periodate-oxidized celluloses.
32466232	3	51	theme	common	477:482	arg1	"					526:526	the common cellulose derivative "dialdehyde cellulose"	473:526	the common cellulose derivative "dialdehyde cellulose" (DAC)	473:532	Silanization has been widely used for cellulosic and nano-structured celluloses, but was a problem so far if to be applied to the common cellulose derivative "dialdehyde cellulose" (DAC), i.e., highly periodate-oxidized celluloses.
32466232	7	52	theme	mild	1221:1224	arg1	conditions					1235:1244	mild reaction conditions	1221:1244	mild reaction conditions	1221:1244	The modification was achieved in aqueous medium under mild reaction conditions.
32466232	8	53	theme	dialdehyde	1403:1412	arg1	structure					1424:1432	the modified dialdehyde cellulose structure	1390:1432	the modified dialdehyde cellulose structure	1390:1432	Different treatments cause different levels of hydrolysis of the organosilane compound, which resulted in diverse condensed silica networks in the modified dialdehyde cellulose structure.
32466232	6	54	theme	group	1017:1021	arg1	links					995:999	links	995:999	links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C)	995:1080	The cross-linking involves both links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C) and imine-type bonds between the amino group and keto functions of the DAC (-HC=N-).
32466232	6	54	theme	group	1017:1021	arg1	bonds					1097:1101	imine-type bonds	1086:1101	imine-type bonds between the amino group and keto functions of the DAC (-HC=N-)	1086:1164	The cross-linking involves both links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C) and imine-type bonds between the amino group and keto functions of the DAC (-HC=N-).
32466232	1	55	theme	biomaterials	128:139	arg1	field					119:123	the field	115:123	the field of biomaterials and composites	115:154	Cellulose derivatives have many potential applications in the field of biomaterials and composites, in addition to several ways of modification leading to them.
32466232	1	56	theme	Cellulose	57:65	arg1	derivatives					67:77	Cellulose derivatives	57:77	Cellulose derivatives	57:77	Cellulose derivatives have many potential applications in the field of biomaterials and composites, in addition to several ways of modification leading to them.
32466232	2	57	theme	organic-inorganic	311:327	arg1	materials					336:344	multipurpose and organic-inorganic hybrid materials	294:344	multipurpose and organic-inorganic hybrid materials	294:344	Silanization in aqueous media is one of the most promising routes to create multipurpose and organic-inorganic hybrid materials.
32466232	8	58	theme	organosilane	1312:1323	arg1	compound					1325:1332	the organosilane compound	1308:1332	the organosilane compound	1308:1332	Different treatments cause different levels of hydrolysis of the organosilane compound, which resulted in diverse condensed silica networks in the modified dialdehyde cellulose structure.
32466232	2	59	theme	hybrid	329:334	arg1	materials					336:344	multipurpose and organic-inorganic hybrid materials	294:344	multipurpose and organic-inorganic hybrid materials	294:344	Silanization in aqueous media is one of the most promising routes to create multipurpose and organic-inorganic hybrid materials.
32466232	4	60	theme	straightforward	595:609	arg1	protocol					624:631	a straightforward silanization protocol	593:631	a straightforward silanization protocol for dialdehyde cellulose	593:656	In this work, a straightforward silanization protocol for dialdehyde cellulose is proposed, which can be readily modified with (3-aminopropyl)triethoxysilane.
32466232	1	61	theme	composites	145:154	arg1	field					119:123	the field	115:123	the field of biomaterials and composites	115:154	Cellulose derivatives have many potential applications in the field of biomaterials and composites, in addition to several ways of modification leading to them.
32466232	3	62	theme	cellulosic	385:394	arg1	celluloses					416:425	cellulosic and nano-structured celluloses	385:425	cellulosic and nano-structured celluloses	385:425	Silanization has been widely used for cellulosic and nano-structured celluloses, but was a problem so far if to be applied to the common cellulose derivative "dialdehyde cellulose" (DAC), i.e., highly periodate-oxidized celluloses.
32466232	6	63	theme	hydroxyl	1008:1015	arg1	cellulose					1039:1047	the oxidized cellulose	1026:1047	the oxidized cellulose	1026:1047	The cross-linking involves both links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C) and imine-type bonds between the amino group and keto functions of the DAC (-HC=N-).
32466232	6	63	theme	hydroxyl	1008:1015	arg1	group					1017:1021	the hydroxyl group	1004:1021	the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C)	1004:1080	The cross-linking involves both links of the hydroxyl group of the oxidized cellulose with the silanol groups (Si-O-C) and imine-type bonds between the amino group and keto functions of the DAC (-HC=N-).
32466232	8	64	theme	cellulose	1414:1422	arg1	structure					1424:1432	the modified dialdehyde cellulose structure	1390:1432	the modified dialdehyde cellulose structure	1390:1432	Different treatments cause different levels of hydrolysis of the organosilane compound, which resulted in diverse condensed silica networks in the modified dialdehyde cellulose structure.
32466232	7	65	theme	reaction	1226:1233	arg1	conditions					1235:1244	mild reaction conditions	1221:1244	mild reaction conditions	1221:1244	The modification was achieved in aqueous medium under mild reaction conditions.
32466232	3	66	theme	dialdehyde	506:515	arg1	DAC					529:531	DAC	529:531	DAC	529:531	Silanization has been widely used for cellulosic and nano-structured celluloses, but was a problem so far if to be applied to the common cellulose derivative "dialdehyde cellulose" (DAC), i.e., highly periodate-oxidized celluloses.
32466232	3	66	theme	dialdehyde	506:515	arg1	"					526:526	the common cellulose derivative "dialdehyde cellulose"	473:526	the common cellulose derivative "dialdehyde cellulose" (DAC)	473:532	Silanization has been widely used for cellulosic and nano-structured celluloses, but was a problem so far if to be applied to the common cellulose derivative "dialdehyde cellulose" (DAC), i.e., highly periodate-oxidized celluloses.
32466232	4	67	mod	modified	692:699	arg1	which					671:675	which	671:675	which	671:675	In this work, a straightforward silanization protocol for dialdehyde cellulose is proposed, which can be readily modified with (3-aminopropyl)triethoxysilane.
32466232	4	67	mod	modified	692:699	arg3	triethoxysilane					721:735	(3-aminopropyl)triethoxysilane	706:735	(3-aminopropyl)triethoxysilane	706:735	In this work, a straightforward silanization protocol for dialdehyde cellulose is proposed, which can be readily modified with (3-aminopropyl)triethoxysilane.
35181098	0	0	theme	microbiota	99:108	arg1	homeostasis					110:120	gut microbiota homeostasis	95:120	gut microbiota homeostasis	95:120	Alginate oligosaccharides protect against fumonisin B1-induced intestinal damage via promoting gut microbiota homeostasis.
35181098	5	1	theme	epithelial	629:638	arg1	function					648:655	intestinal epithelial barrier function	618:655	intestinal epithelial barrier function	618:655	Results show that FB1 degraded intestinal epithelial barrier function as evidenced by increased pathological epithelial cell shedding, reduced the number of goblet cells, and promoted intestinal cell apoptosis.
35181098	1	2	theme	intestinal	231:240	arg1	degradation					250:260	intestinal barrier degradation	231:260	intestinal barrier degradation	231:260	Fumonisin B1 (FB1), one of the most common mycotoxins contaminating feed and food, has been shown to induce intestinal barrier degradation.
35181098	0	3	theme	gut	95:97	arg1	homeostasis					110:120	gut microbiota homeostasis	95:120	gut microbiota homeostasis	95:120	Alginate oligosaccharides protect against fumonisin B1-induced intestinal damage via promoting gut microbiota homeostasis.
35181098	12	4	theme	FB1-induced	1573:1583	arg1	dysbiosis					1599:1607	FB1-induced gut microbial dysbiosis	1573:1607	FB1-induced gut microbial dysbiosis	1573:1607	We have thus provided a novel insight into the protective role of AOS on FB1-induced gut microbial dysbiosis.
35181098	5	5	theme	barrier	640:646	arg1	function					648:655	intestinal epithelial barrier function	618:655	intestinal epithelial barrier function	618:655	Results show that FB1 degraded intestinal epithelial barrier function as evidenced by increased pathological epithelial cell shedding, reduced the number of goblet cells, and promoted intestinal cell apoptosis.
35181098	4	6	theme	gut	553:555	arg1	microbiota					557:566	gut microbiota	553:566	gut microbiota upon FB1 exposure	553:584	However, little is known about the beneficial effect of AOS on gut microbiota upon FB1 exposure.
35181098	3	7	theme	Alginate	333:340	arg1	AOS					360:362	AOS	360:362	AOS	360:362	Alginate oligosaccharides (AOS) have been reported to exert their anti-inflammatory and anti-apoptotic function partially via modulation the gut microbiota.
35181098	3	7	theme	Alginate	333:340	arg1	oligosaccharides					342:357	Alginate oligosaccharides	333:357	Alginate oligosaccharides (AOS)	333:363	Alginate oligosaccharides (AOS) have been reported to exert their anti-inflammatory and anti-apoptotic function partially via modulation the gut microbiota.
35181098	10	8	theme	microbes	1230:1237	arg1	growth					1209:1214	the growth	1205:1214	the growth of beneficial microbes such as Roseburia, Bifidobacterium, and Akkermansia	1205:1289	AOS alleviated gut microbial dysbiosis by promoting the growth of beneficial microbes such as Roseburia, Bifidobacterium, and Akkermansia, and increasing SCFAs production upon FB1 exposure.
35181098	4	9	theme	FB1	573:575	arg1	exposure					577:584	FB1 exposure	573:584	FB1 exposure	573:584	However, little is known about the beneficial effect of AOS on gut microbiota upon FB1 exposure.
35181098	11	10	theme	AOS-treated	1399:1409	arg1	alteration					1426:1435	FB1- and AOS-treated gut microbiota alteration	1390:1435	FB1- and AOS-treated gut microbiota alteration	1390:1435	Moreover, the correlation analysis showed that FB1- and AOS-treated gut microbiota alteration is closely associated with the change of intestinal phenotype.
35181098	7	11	theme	beneficial	947:956	arg1	microbes					958:965	beneficial microbes	947:965	beneficial microbes	947:965	FB1 increased the level of Lactobacillus and decreased the relative abundance of beneficial microbes.
35181098	9	12	theme	FB1-induced	1065:1075	arg1	damage					1088:1093	FB1-induced intestinal damage	1065:1093	FB1-induced intestinal damage	1065:1093	AOS greatly ameliorated FB1-induced intestinal damage, inflammation, and oxidative stress (eg., T-SOD and MDA).
35181098	10	13	theme	microbial	1172:1180	arg1	dysbiosis					1182:1190	gut microbial dysbiosis	1168:1190	gut microbial dysbiosis	1168:1190	AOS alleviated gut microbial dysbiosis by promoting the growth of beneficial microbes such as Roseburia, Bifidobacterium, and Akkermansia, and increasing SCFAs production upon FB1 exposure.
35181098	7	14	theme	microbes	958:965	arg1	abundance					934:942	the relative abundance	921:942	the relative abundance of beneficial microbes	921:965	FB1 increased the level of Lactobacillus and decreased the relative abundance of beneficial microbes.
35181098	9	15	theme	intestinal	1077:1086	arg1	damage					1088:1093	FB1-induced intestinal damage	1065:1093	FB1-induced intestinal damage	1065:1093	AOS greatly ameliorated FB1-induced intestinal damage, inflammation, and oxidative stress (eg., T-SOD and MDA).
35181098	1	16	theme	common	159:164	arg1	mycotoxins					166:175	the most common mycotoxins	150:175	the most common mycotoxins contaminating feed and food	150:203	Fumonisin B1 (FB1), one of the most common mycotoxins contaminating feed and food, has been shown to induce intestinal barrier degradation.
35181098	11	17	theme	FB1-	1390:1393	arg1	alteration					1426:1435	FB1- and AOS-treated gut microbiota alteration	1390:1435	FB1- and AOS-treated gut microbiota alteration	1390:1435	Moreover, the correlation analysis showed that FB1- and AOS-treated gut microbiota alteration is closely associated with the change of intestinal phenotype.
35181098	8	18	theme	short	1008:1012	arg1	SCFAs					1033:1037	SCFAs	1033:1037	SCFAs	1033:1037	FB1 largely inhibited the production of short chain fatty acids (SCFAs).
35181098	8	18	theme	short	1008:1012	arg1	acids					1026:1030	short chain fatty acids	1008:1030	short chain fatty acids (SCFAs)	1008:1038	FB1 largely inhibited the production of short chain fatty acids (SCFAs).
35181098	9	19	dep	stress	1124:1129	arg1	eg.					1132:1134	eg.	1132:1134	eg.	1132:1134	AOS greatly ameliorated FB1-induced intestinal damage, inflammation, and oxidative stress (eg., T-SOD and MDA).
35181098	9	19	dep	stress	1124:1129	arg1	MDA					1147:1149	MDA	1147:1149	MDA	1147:1149	AOS greatly ameliorated FB1-induced intestinal damage, inflammation, and oxidative stress (eg., T-SOD and MDA).
35181098	9	19	dep	stress	1124:1129	arg1	T-SOD					1137:1141	T-SOD	1137:1141	T-SOD	1137:1141	AOS greatly ameliorated FB1-induced intestinal damage, inflammation, and oxidative stress (eg., T-SOD and MDA).
35181098	1	20	theme	mycotoxins	166:175	arg1	mycotoxins					166:175	the most common mycotoxins	150:175	the most common mycotoxins contaminating feed and food	150:203	Fumonisin B1 (FB1), one of the most common mycotoxins contaminating feed and food, has been shown to induce intestinal barrier degradation.
35181098	1	20	theme	mycotoxins	166:175	arg1	one					143:145	one	143:145	one	143:145	Fumonisin B1 (FB1), one of the most common mycotoxins contaminating feed and food, has been shown to induce intestinal barrier degradation.
35181098	1	20	theme	mycotoxins	166:175	arg1	B1					133:134	Fumonisin B1	123:134	Fumonisin B1 (FB1)	123:140	Fumonisin B1 (FB1), one of the most common mycotoxins contaminating feed and food, has been shown to induce intestinal barrier degradation.
35181098	11	21	theme	correlation	1357:1367	arg1	analysis					1369:1376	the correlation analysis	1353:1376	the correlation analysis	1353:1376	Moreover, the correlation analysis showed that FB1- and AOS-treated gut microbiota alteration is closely associated with the change of intestinal phenotype.
35181098	11	22	theme	microbiota	1415:1424	arg1	alteration					1426:1435	FB1- and AOS-treated gut microbiota alteration	1390:1435	FB1- and AOS-treated gut microbiota alteration	1390:1435	Moreover, the correlation analysis showed that FB1- and AOS-treated gut microbiota alteration is closely associated with the change of intestinal phenotype.
35181098	0	23	theme	Alginate	0:7	arg1	oligosaccharides					9:24	Alginate oligosaccharides	0:24	Alginate oligosaccharides	0:24	Alginate oligosaccharides protect against fumonisin B1-induced intestinal damage via promoting gut microbiota homeostasis.
35181098	12	24	theme	microbial	1589:1597	arg1	dysbiosis					1599:1607	FB1-induced gut microbial dysbiosis	1573:1607	FB1-induced gut microbial dysbiosis	1573:1607	We have thus provided a novel insight into the protective role of AOS on FB1-induced gut microbial dysbiosis.
35181098	5	25	theme	intestinal	618:627	arg1	function					648:655	intestinal epithelial barrier function	618:655	intestinal epithelial barrier function	618:655	Results show that FB1 degraded intestinal epithelial barrier function as evidenced by increased pathological epithelial cell shedding, reduced the number of goblet cells, and promoted intestinal cell apoptosis.
35181098	8	26	theme	fatty	1020:1024	arg1	SCFAs					1033:1037	SCFAs	1033:1037	SCFAs	1033:1037	FB1 largely inhibited the production of short chain fatty acids (SCFAs).
35181098	8	26	theme	fatty	1020:1024	arg1	acids					1026:1030	short chain fatty acids	1008:1030	short chain fatty acids (SCFAs)	1008:1038	FB1 largely inhibited the production of short chain fatty acids (SCFAs).
35181098	3	27	theme	anti-inflammatory	399:415	arg1	function					436:443	their anti-inflammatory and anti-apoptotic function	393:443	their anti-inflammatory and anti-apoptotic function	393:443	Alginate oligosaccharides (AOS) have been reported to exert their anti-inflammatory and anti-apoptotic function partially via modulation the gut microbiota.
35181098	12	28	theme	gut	1585:1587	arg1	dysbiosis					1599:1607	FB1-induced gut microbial dysbiosis	1573:1607	FB1-induced gut microbial dysbiosis	1573:1607	We have thus provided a novel insight into the protective role of AOS on FB1-induced gut microbial dysbiosis.
35181098	5	29	theme	epithelial	696:705	arg1	shedding					712:719	pathological epithelial cell shedding	683:719	pathological epithelial cell shedding	683:719	Results show that FB1 degraded intestinal epithelial barrier function as evidenced by increased pathological epithelial cell shedding, reduced the number of goblet cells, and promoted intestinal cell apoptosis.
35181098	7	30	theme	relative	925:932	arg1	abundance					934:942	the relative abundance	921:942	the relative abundance of beneficial microbes	921:965	FB1 increased the level of Lactobacillus and decreased the relative abundance of beneficial microbes.
35181098	2	31	theme	gut	284:286	arg1	microbiota					288:297	gut microbiota	284:297	gut microbiota	284:297	However, its role on gut microbiota in this process is still unclear.
35181098	11	32	theme	intestinal	1478:1487	arg1	phenotype					1489:1497	intestinal phenotype	1478:1497	intestinal phenotype	1478:1497	Moreover, the correlation analysis showed that FB1- and AOS-treated gut microbiota alteration is closely associated with the change of intestinal phenotype.
35181098	4	33	from	effect	536:541	arg1	microbiota					557:566	gut microbiota	553:566	gut microbiota upon FB1 exposure	553:584	However, little is known about the beneficial effect of AOS on gut microbiota upon FB1 exposure.
35181098	11	34	theme	phenotype	1489:1497	arg1	change					1468:1473	the change	1464:1473	the change of intestinal phenotype	1464:1497	Moreover, the correlation analysis showed that FB1- and AOS-treated gut microbiota alteration is closely associated with the change of intestinal phenotype.
35181098	5	35	theme	cell	707:710	arg1	shedding					712:719	pathological epithelial cell shedding	683:719	pathological epithelial cell shedding	683:719	Results show that FB1 degraded intestinal epithelial barrier function as evidenced by increased pathological epithelial cell shedding, reduced the number of goblet cells, and promoted intestinal cell apoptosis.
35181098	9	36	theme	oxidative	1114:1122	arg1	stress					1124:1129	oxidative stress	1114:1129	oxidative stress (eg., T-SOD and MDA)	1114:1150	AOS greatly ameliorated FB1-induced intestinal damage, inflammation, and oxidative stress (eg., T-SOD and MDA).
35181098	0	37	theme	B1-induced	52:61	arg1	damage					74:79	fumonisin B1-induced intestinal damage	42:79	fumonisin B1-induced intestinal damage	42:79	Alginate oligosaccharides protect against fumonisin B1-induced intestinal damage via promoting gut microbiota homeostasis.
35181098	4	38	theme	AOS	546:548	arg1	effect					536:541	the beneficial effect	521:541	the beneficial effect of AOS on gut microbiota upon FB1 exposure	521:584	However, little is known about the beneficial effect of AOS on gut microbiota upon FB1 exposure.
35181098	7	39	theme	Lactobacillus	893:905	arg1	level					884:888	the level	880:888	the level of Lactobacillus	880:905	FB1 increased the level of Lactobacillus and decreased the relative abundance of beneficial microbes.
35181098	0	40	theme	fumonisin	42:50	arg1	damage					74:79	fumonisin B1-induced intestinal damage	42:79	fumonisin B1-induced intestinal damage	42:79	Alginate oligosaccharides protect against fumonisin B1-induced intestinal damage via promoting gut microbiota homeostasis.
35181098	12	41	theme	AOS	1566:1568	arg1	role					1558:1561	the protective role	1543:1561	the protective role of AOS on FB1-induced gut microbial dysbiosis	1543:1607	We have thus provided a novel insight into the protective role of AOS on FB1-induced gut microbial dysbiosis.
35181098	1	42	theme	barrier	242:248	arg1	degradation					250:260	intestinal barrier degradation	231:260	intestinal barrier degradation	231:260	Fumonisin B1 (FB1), one of the most common mycotoxins contaminating feed and food, has been shown to induce intestinal barrier degradation.
35181098	6	43	theme	microbiota	842:851	arg1	composition					853:863	the cecal and fecal microbiota composition	822:863	the cecal and fecal microbiota composition	822:863	Markedly, FB1 disturbed the cecal and fecal microbiota composition.
35181098	10	44	theme	FB1	1329:1331	arg1	exposure					1333:1340	FB1 exposure	1329:1340	FB1 exposure	1329:1340	AOS alleviated gut microbial dysbiosis by promoting the growth of beneficial microbes such as Roseburia, Bifidobacterium, and Akkermansia, and increasing SCFAs production upon FB1 exposure.
35181098	5	45	theme	cell	782:785	arg1	apoptosis					787:795	intestinal cell apoptosis	771:795	intestinal cell apoptosis	771:795	Results show that FB1 degraded intestinal epithelial barrier function as evidenced by increased pathological epithelial cell shedding, reduced the number of goblet cells, and promoted intestinal cell apoptosis.
35181098	6	46	theme	fecal	836:840	arg1	composition					853:863	the cecal and fecal microbiota composition	822:863	the cecal and fecal microbiota composition	822:863	Markedly, FB1 disturbed the cecal and fecal microbiota composition.
35181098	0	47	theme	intestinal	63:72	arg1	damage					74:79	fumonisin B1-induced intestinal damage	42:79	fumonisin B1-induced intestinal damage	42:79	Alginate oligosaccharides protect against fumonisin B1-induced intestinal damage via promoting gut microbiota homeostasis.
35181098	4	48	theme	beneficial	525:534	arg1	effect					536:541	the beneficial effect	521:541	the beneficial effect of AOS on gut microbiota upon FB1 exposure	521:584	However, little is known about the beneficial effect of AOS on gut microbiota upon FB1 exposure.
35181098	12	49	from	role	1558:1561	arg1	dysbiosis					1599:1607	FB1-induced gut microbial dysbiosis	1573:1607	FB1-induced gut microbial dysbiosis	1573:1607	We have thus provided a novel insight into the protective role of AOS on FB1-induced gut microbial dysbiosis.
35181098	10	50	theme	beneficial	1219:1228	arg1	Roseburia					1247:1255	Roseburia	1247:1255	Roseburia	1247:1255	AOS alleviated gut microbial dysbiosis by promoting the growth of beneficial microbes such as Roseburia, Bifidobacterium, and Akkermansia, and increasing SCFAs production upon FB1 exposure.
35181098	10	50	theme	beneficial	1219:1228	arg1	Bifidobacterium					1258:1272	Bifidobacterium	1258:1272	Bifidobacterium	1258:1272	AOS alleviated gut microbial dysbiosis by promoting the growth of beneficial microbes such as Roseburia, Bifidobacterium, and Akkermansia, and increasing SCFAs production upon FB1 exposure.
35181098	10	50	theme	beneficial	1219:1228	arg1	microbes					1230:1237	beneficial microbes	1219:1237	beneficial microbes such as Roseburia, Bifidobacterium, and Akkermansia	1219:1289	AOS alleviated gut microbial dysbiosis by promoting the growth of beneficial microbes such as Roseburia, Bifidobacterium, and Akkermansia, and increasing SCFAs production upon FB1 exposure.
35181098	10	50	theme	beneficial	1219:1228	arg1	Akkermansia					1279:1289	Akkermansia	1279:1289	Akkermansia	1279:1289	AOS alleviated gut microbial dysbiosis by promoting the growth of beneficial microbes such as Roseburia, Bifidobacterium, and Akkermansia, and increasing SCFAs production upon FB1 exposure.
35181098	8	51	theme	chain	1014:1018	arg1	SCFAs					1033:1037	SCFAs	1033:1037	SCFAs	1033:1037	FB1 largely inhibited the production of short chain fatty acids (SCFAs).
35181098	8	51	theme	chain	1014:1018	arg1	acids					1026:1030	short chain fatty acids	1008:1030	short chain fatty acids (SCFAs)	1008:1038	FB1 largely inhibited the production of short chain fatty acids (SCFAs).
35181098	6	52	theme	cecal	826:830	arg1	composition					853:863	the cecal and fecal microbiota composition	822:863	the cecal and fecal microbiota composition	822:863	Markedly, FB1 disturbed the cecal and fecal microbiota composition.
35181098	5	53	theme	intestinal	771:780	arg1	apoptosis					787:795	intestinal cell apoptosis	771:795	intestinal cell apoptosis	771:795	Results show that FB1 degraded intestinal epithelial barrier function as evidenced by increased pathological epithelial cell shedding, reduced the number of goblet cells, and promoted intestinal cell apoptosis.
35181098	10	54	theme	SCFAs	1307:1311	arg1	production					1313:1322	SCFAs production	1307:1322	SCFAs production upon FB1 exposure	1307:1340	AOS alleviated gut microbial dysbiosis by promoting the growth of beneficial microbes such as Roseburia, Bifidobacterium, and Akkermansia, and increasing SCFAs production upon FB1 exposure.
35181098	1	55	theme	Fumonisin	123:131	arg1	mycotoxins					166:175	the most common mycotoxins	150:175	the most common mycotoxins contaminating feed and food	150:203	Fumonisin B1 (FB1), one of the most common mycotoxins contaminating feed and food, has been shown to induce intestinal barrier degradation.
35181098	1	55	theme	Fumonisin	123:131	arg1	FB1					137:139	FB1	137:139	FB1	137:139	Fumonisin B1 (FB1), one of the most common mycotoxins contaminating feed and food, has been shown to induce intestinal barrier degradation.
35181098	1	55	theme	Fumonisin	123:131	arg1	one					143:145	one	143:145	one	143:145	Fumonisin B1 (FB1), one of the most common mycotoxins contaminating feed and food, has been shown to induce intestinal barrier degradation.
35181098	1	55	theme	Fumonisin	123:131	arg1	B1					133:134	Fumonisin B1	123:134	Fumonisin B1 (FB1)	123:140	Fumonisin B1 (FB1), one of the most common mycotoxins contaminating feed and food, has been shown to induce intestinal barrier degradation.
35181098	10	56	theme	gut	1168:1170	arg1	dysbiosis					1182:1190	gut microbial dysbiosis	1168:1190	gut microbial dysbiosis	1168:1190	AOS alleviated gut microbial dysbiosis by promoting the growth of beneficial microbes such as Roseburia, Bifidobacterium, and Akkermansia, and increasing SCFAs production upon FB1 exposure.
35181098	5	57	theme	pathological	683:694	arg1	shedding					712:719	pathological epithelial cell shedding	683:719	pathological epithelial cell shedding	683:719	Results show that FB1 degraded intestinal epithelial barrier function as evidenced by increased pathological epithelial cell shedding, reduced the number of goblet cells, and promoted intestinal cell apoptosis.
35181098	3	58	theme	gut	474:476	arg1	microbiota					478:487	the gut microbiota	470:487	modulation the gut microbiota	459:487	Alginate oligosaccharides (AOS) have been reported to exert their anti-inflammatory and anti-apoptotic function partially via modulation the gut microbiota.
35181098	2	59	from	role	276:279	arg1	microbiota					288:297	gut microbiota	284:297	gut microbiota	284:297	However, its role on gut microbiota in this process is still unclear.
35181098	2	59	from	role	276:279	arg1	process					307:313	this process	302:313	this process	302:313	However, its role on gut microbiota in this process is still unclear.
35181098	11	60	theme	gut	1411:1413	arg1	alteration					1426:1435	FB1- and AOS-treated gut microbiota alteration	1390:1435	FB1- and AOS-treated gut microbiota alteration	1390:1435	Moreover, the correlation analysis showed that FB1- and AOS-treated gut microbiota alteration is closely associated with the change of intestinal phenotype.
35181098	5	61	theme	goblet	744:749	arg1	cells					751:755	goblet cells	744:755	goblet cells	744:755	Results show that FB1 degraded intestinal epithelial barrier function as evidenced by increased pathological epithelial cell shedding, reduced the number of goblet cells, and promoted intestinal cell apoptosis.
35181098	12	62	theme	novel	1524:1528	arg1	insight					1530:1536	a novel insight	1522:1536	a novel insight into the protective role of AOS on FB1-induced gut microbial dysbiosis	1522:1607	We have thus provided a novel insight into the protective role of AOS on FB1-induced gut microbial dysbiosis.
35181098	3	63	theme	anti-apoptotic	421:434	arg1	function					436:443	their anti-inflammatory and anti-apoptotic function	393:443	their anti-inflammatory and anti-apoptotic function	393:443	Alginate oligosaccharides (AOS) have been reported to exert their anti-inflammatory and anti-apoptotic function partially via modulation the gut microbiota.
35181098	8	64	theme	acids	1026:1030	arg1	production					994:1003	the production	990:1003	the production of short chain fatty acids (SCFAs)	990:1038	FB1 largely inhibited the production of short chain fatty acids (SCFAs).
35181098	5	65	theme	cells	751:755	arg1	number					734:739	the number	730:739	the number of goblet cells	730:755	Results show that FB1 degraded intestinal epithelial barrier function as evidenced by increased pathological epithelial cell shedding, reduced the number of goblet cells, and promoted intestinal cell apoptosis.
35181098	3	66	dep	modulation	459:468	arg1	microbiota					478:487	the gut microbiota	470:487	modulation the gut microbiota	459:487	Alginate oligosaccharides (AOS) have been reported to exert their anti-inflammatory and anti-apoptotic function partially via modulation the gut microbiota.
35181098	12	67	theme	protective	1547:1556	arg1	role					1558:1561	the protective role	1543:1561	the protective role of AOS on FB1-induced gut microbial dysbiosis	1543:1607	We have thus provided a novel insight into the protective role of AOS on FB1-induced gut microbial dysbiosis.
33307544	5	0	theme	cuticle	846:852	arg1	analysis					830:837	Imaging analysis	822:837	Imaging analysis of the cuticle	822:852	Imaging analysis of the cuticle reveals an intricate multilayer laminated structure, with varying chitin-protein fiber orientations, arranged in eight hierarchical levels.
33307544	2	1	from	nanoplatelets	285:297	arg1	structures					234:243	these structures	228:243	these structures	228:243	In these structures, the basic components are nanofibers or nanoplatelets, which are strong and stiff but anisotropic, whereas at the macrolevel, isotropy is preferred because the direction and magnitude of loads is unpredictable.
33307544	4	2	theme	toughness	811:819	arg1	isotropy					770:777	the macroscale isotropy	755:777	the macroscale isotropy of the strength, stiffness, and toughness	755:819	Focusing on the tibia (fixed finger) of the scorpion pincer, bending tests of cuticle samples confirm the macroscale isotropy of the strength, stiffness, and toughness.
33307544	2	3	dep	nanofibers	271:280	arg1	stiff					321:325	stiff	321:325	stiff	321:325	In these structures, the basic components are nanofibers or nanoplatelets, which are strong and stiff but anisotropic, whereas at the macrolevel, isotropy is preferred because the direction and magnitude of loads is unpredictable.
33307544	2	3	dep	nanofibers	271:280	arg1	strong					310:315	strong	310:315	strong	310:315	In these structures, the basic components are nanofibers or nanoplatelets, which are strong and stiff but anisotropic, whereas at the macrolevel, isotropy is preferred because the direction and magnitude of loads is unpredictable.
33307544	2	3	dep	nanofibers	271:280	arg1	whereas					344:350	whereas	344:350	whereas	344:350	In these structures, the basic components are nanofibers or nanoplatelets, which are strong and stiff but anisotropic, whereas at the macrolevel, isotropy is preferred because the direction and magnitude of loads is unpredictable.
33307544	2	3	dep	nanofibers	271:280	arg1	anisotropic					331:341	anisotropic	331:341	anisotropic	331:341	In these structures, the basic components are nanofibers or nanoplatelets, which are strong and stiff but anisotropic, whereas at the macrolevel, isotropy is preferred because the direction and magnitude of loads is unpredictable.
33307544	7	4	theme	different	1321:1329	arg1	scales					1338:1343	different length scales	1321:1343	different length scales	1321:1343	Using laminate analysis to model the cuticle structure, we were able to correlate the nanostructure to the macro-mechanical properties, uncovering shear enhancing mechanisms at different length scales.
33307544	10	5	theme	composites	1672:1681	arg1	design					1645:1650	the design	1641:1650	the design of future synthetic composites with balanced strength, stiffness, toughness, and isotropy	1641:1740	A similar structural approach can be adopted to the design of future synthetic composites with balanced strength, stiffness, toughness, and isotropy.
33307544	7	6	theme	length	1331:1336	arg1	scales					1338:1343	different length scales	1321:1343	different length scales	1321:1343	Using laminate analysis to model the cuticle structure, we were able to correlate the nanostructure to the macro-mechanical properties, uncovering shear enhancing mechanisms at different length scales.
33307544	5	7	theme	chitin-protein	920:933	arg1	orientations					941:952	varying chitin-protein fiber orientations	912:952	varying chitin-protein fiber orientations	912:952	Imaging analysis of the cuticle reveals an intricate multilayer laminated structure, with varying chitin-protein fiber orientations, arranged in eight hierarchical levels.
33307544	3	8	from	anisotropy	528:537	arg1	transition					512:521	the transition	508:521	the transition from anisotropy to isotropy	508:549	The structural features and mechanisms, which drive the transition from anisotropy to isotropy across length scales, raise fundamental questions and are therefore the subject of the current study.
33307544	10	9	theme	future	1655:1660	arg1	composites					1672:1681	future synthetic composites	1655:1681	future synthetic composites with balanced strength, stiffness, toughness, and isotropy	1655:1740	A similar structural approach can be adopted to the design of future synthetic composites with balanced strength, stiffness, toughness, and isotropy.
33307544	9	10	theme	matrix	1545:1550	arg1	strength					1558:1565	the protein matrix shear strength	1533:1565	the protein matrix shear strength	1533:1565	Interlaminar failure (ILF) analysis of the cuticle leads to an estimation of the protein matrix shear strength, previously not measured.
33307544	5	11	theme	fiber	935:939	arg1	orientations					941:952	varying chitin-protein fiber orientations	912:952	varying chitin-protein fiber orientations	912:952	Imaging analysis of the cuticle reveals an intricate multilayer laminated structure, with varying chitin-protein fiber orientations, arranged in eight hierarchical levels.
33307544	4	12	theme	bending	714:720	arg1	tests					722:726	bending tests	714:726	bending tests of cuticle samples	714:745	Focusing on the tibia (fixed finger) of the scorpion pincer, bending tests of cuticle samples confirm the macroscale isotropy of the strength, stiffness, and toughness.
33307544	5	13	theme	laminated	886:894	arg1	structure					896:904	an intricate multilayer laminated structure	862:904	an intricate multilayer laminated structure	862:904	Imaging analysis of the cuticle reveals an intricate multilayer laminated structure, with varying chitin-protein fiber orientations, arranged in eight hierarchical levels.
33307544	10	14	theme	synthetic	1662:1670	arg1	composites					1672:1681	future synthetic composites	1655:1681	future synthetic composites with balanced strength, stiffness, toughness, and isotropy	1655:1740	A similar structural approach can be adopted to the design of future synthetic composites with balanced strength, stiffness, toughness, and isotropy.
33307544	4	15	theme	stiffness	796:804	arg1	isotropy					770:777	the macroscale isotropy	755:777	the macroscale isotropy of the strength, stiffness, and toughness	755:819	Focusing on the tibia (fixed finger) of the scorpion pincer, bending tests of cuticle samples confirm the macroscale isotropy of the strength, stiffness, and toughness.
33307544	7	16	theme	macro-mechanical	1251:1266	arg1	properties					1268:1277	the macro-mechanical properties	1247:1277	the macro-mechanical properties	1247:1277	Using laminate analysis to model the cuticle structure, we were able to correlate the nanostructure to the macro-mechanical properties, uncovering shear enhancing mechanisms at different length scales.
33307544	4	17	theme	cuticle	731:737	arg1	samples					739:745	cuticle samples	731:745	cuticle samples	731:745	Focusing on the tibia (fixed finger) of the scorpion pincer, bending tests of cuticle samples confirm the macroscale isotropy of the strength, stiffness, and toughness.
33307544	7	18	theme	enhancing	1297:1305	arg1	mechanisms					1307:1316	shear enhancing mechanisms	1291:1316	shear enhancing mechanisms	1291:1316	Using laminate analysis to model the cuticle structure, we were able to correlate the nanostructure to the macro-mechanical properties, uncovering shear enhancing mechanisms at different length scales.
33307544	2	19	dep	direction	405:413	arg1	the					401:403	the	401:403	the	401:403	In these structures, the basic components are nanofibers or nanoplatelets, which are strong and stiff but anisotropic, whereas at the macrolevel, isotropy is preferred because the direction and magnitude of loads is unpredictable.
33307544	1	20	theme	Biological	73:82	arg1	exoskeletons					119:130	exoskeletons	119:130	exoskeletons	119:130	Biological structures such as bone, nacre and exoskeletons are organized hierarchically, with the degree of isotropy correlating with the length-scale.
33307544	1	20	theme	Biological	73:82	arg1	nacre					109:113	nacre	109:113	nacre	109:113	Biological structures such as bone, nacre and exoskeletons are organized hierarchically, with the degree of isotropy correlating with the length-scale.
33307544	1	20	theme	Biological	73:82	arg1	structures					84:93	Biological structures	73:93	Biological structures such as bone, nacre and exoskeletons	73:130	Biological structures such as bone, nacre and exoskeletons are organized hierarchically, with the degree of isotropy correlating with the length-scale.
33307544	1	20	theme	Biological	73:82	arg1	bone					103:106	bone	103:106	bone	103:106	Biological structures such as bone, nacre and exoskeletons are organized hierarchically, with the degree of isotropy correlating with the length-scale.
33307544	7	21	theme	shear	1291:1295	arg1	mechanisms					1307:1316	shear enhancing mechanisms	1291:1316	shear enhancing mechanisms	1291:1316	Using laminate analysis to model the cuticle structure, we were able to correlate the nanostructure to the macro-mechanical properties, uncovering shear enhancing mechanisms at different length scales.
33307544	4	22	theme	scorpion	697:704	arg1	pincer					706:711	the scorpion pincer	693:711	the scorpion pincer	693:711	Focusing on the tibia (fixed finger) of the scorpion pincer, bending tests of cuticle samples confirm the macroscale isotropy of the strength, stiffness, and toughness.
33307544	5	23	theme	Imaging	822:828	arg1	analysis					830:837	Imaging analysis	822:837	Imaging analysis of the cuticle	822:852	Imaging analysis of the cuticle reveals an intricate multilayer laminated structure, with varying chitin-protein fiber orientations, arranged in eight hierarchical levels.
33307544	0	24	theme	Structural	0:9	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis	0:18	Structural analysis across length scales of the scorpion pincer cuticle.
33307544	3	25	theme	length	558:563	arg1	scales					565:570	length scales	558:570	length scales	558:570	The structural features and mechanisms, which drive the transition from anisotropy to isotropy across length scales, raise fundamental questions and are therefore the subject of the current study.
33307544	10	26	theme	similar	1595:1601	arg1	approach					1614:1621	A similar structural approach	1593:1621	A similar structural approach	1593:1621	A similar structural approach can be adopted to the design of future synthetic composites with balanced strength, stiffness, toughness, and isotropy.
33307544	0	27	theme	length	27:32	arg1	scales					34:39	length scales	27:39	length scales of the scorpion pincer cuticle	27:70	Structural analysis across length scales of the scorpion pincer cuticle.
33307544	4	28	theme	macroscale	759:768	arg1	isotropy					770:777	the macroscale isotropy	755:777	the macroscale isotropy of the strength, stiffness, and toughness	755:819	Focusing on the tibia (fixed finger) of the scorpion pincer, bending tests of cuticle samples confirm the macroscale isotropy of the strength, stiffness, and toughness.
33307544	10	29	theme	structural	1603:1612	arg1	approach					1614:1621	A similar structural approach	1593:1621	A similar structural approach	1593:1621	A similar structural approach can be adopted to the design of future synthetic composites with balanced strength, stiffness, toughness, and isotropy.
33307544	9	30	theme	protein	1537:1543	arg1	strength					1558:1565	the protein matrix shear strength	1533:1565	the protein matrix shear strength	1533:1565	Interlaminar failure (ILF) analysis of the cuticle leads to an estimation of the protein matrix shear strength, previously not measured.
33307544	1	31	theme	isotropy	181:188	arg1	degree					171:176	the degree	167:176	the degree of isotropy correlating with the length-scale	167:222	Biological structures such as bone, nacre and exoskeletons are organized hierarchically, with the degree of isotropy correlating with the length-scale.
33307544	6	32	theme	thick	1073:1077	arg1	layer					1092:1096	a thick intermediate layer	1071:1096	a thick intermediate layer	1071:1096	We show that the cuticle flexural stiffness is increased by the existence of a thick intermediate layer, not seen before in the claws of crustaceans.
33307544	6	33	theme	crustaceans	1131:1141	arg1	claws					1122:1126	the claws	1118:1126	the claws of crustaceans	1118:1141	We show that the cuticle flexural stiffness is increased by the existence of a thick intermediate layer, not seen before in the claws of crustaceans.
33307544	4	34	theme	pincer	706:711	arg1	tibia					669:673	the tibia	665:673	the tibia (fixed finger) of the scorpion pincer	665:711	Focusing on the tibia (fixed finger) of the scorpion pincer, bending tests of cuticle samples confirm the macroscale isotropy of the strength, stiffness, and toughness.
33307544	4	34	theme	pincer	706:711	arg1	finger					682:687	fixed finger	676:687	fixed finger	676:687	Focusing on the tibia (fixed finger) of the scorpion pincer, bending tests of cuticle samples confirm the macroscale isotropy of the strength, stiffness, and toughness.
33307544	9	35	theme	cuticle	1499:1505	arg1	analysis					1483:1490	Interlaminar failure (ILF) analysis	1456:1490	Interlaminar failure (ILF) analysis of the cuticle	1456:1505	Interlaminar failure (ILF) analysis of the cuticle leads to an estimation of the protein matrix shear strength, previously not measured.
33307544	6	36	theme	cuticle	1011:1017	arg1	stiffness					1028:1036	the cuticle flexural stiffness	1007:1036	the cuticle flexural stiffness	1007:1036	We show that the cuticle flexural stiffness is increased by the existence of a thick intermediate layer, not seen before in the claws of crustaceans.
33307544	5	37	theme	intricate	865:873	arg1	structure					896:904	an intricate multilayer laminated structure	862:904	an intricate multilayer laminated structure	862:904	Imaging analysis of the cuticle reveals an intricate multilayer laminated structure, with varying chitin-protein fiber orientations, arranged in eight hierarchical levels.
33307544	6	38	theme	layer	1092:1096	arg1	existence					1058:1066	the existence	1054:1066	the existence of a thick intermediate layer, not seen before in the claws of crustaceans	1054:1141	We show that the cuticle flexural stiffness is increased by the existence of a thick intermediate layer, not seen before in the claws of crustaceans.
33307544	9	39	theme	shear	1552:1556	arg1	strength					1558:1565	the protein matrix shear strength	1533:1565	the protein matrix shear strength	1533:1565	Interlaminar failure (ILF) analysis of the cuticle leads to an estimation of the protein matrix shear strength, previously not measured.
33307544	3	40	theme	fundamental	579:589	arg1	questions					591:599	fundamental questions	579:599	fundamental questions	579:599	The structural features and mechanisms, which drive the transition from anisotropy to isotropy across length scales, raise fundamental questions and are therefore the subject of the current study.
33307544	9	41	theme	Interlaminar	1456:1467	arg1	ILF					1478:1480	ILF	1478:1480	ILF	1478:1480	Interlaminar failure (ILF) analysis of the cuticle leads to an estimation of the protein matrix shear strength, previously not measured.
33307544	9	41	theme	Interlaminar	1456:1467	arg1	failure					1469:1475	Interlaminar failure	1456:1475	Interlaminar failure (ILF) analysis of the cuticle	1456:1505	Interlaminar failure (ILF) analysis of the cuticle leads to an estimation of the protein matrix shear strength, previously not measured.
33307544	0	42	theme	scorpion	48:55	arg1	cuticle					64:70	the scorpion pincer cuticle	44:70	the scorpion pincer cuticle	44:70	Structural analysis across length scales of the scorpion pincer cuticle.
33307544	7	43	theme	cuticle	1181:1187	arg1	structure					1189:1197	the cuticle structure	1177:1197	the cuticle structure	1177:1197	Using laminate analysis to model the cuticle structure, we were able to correlate the nanostructure to the macro-mechanical properties, uncovering shear enhancing mechanisms at different length scales.
33307544	4	44	theme	strength	786:793	arg1	isotropy					770:777	the macroscale isotropy	755:777	the macroscale isotropy of the strength, stiffness, and toughness	755:819	Focusing on the tibia (fixed finger) of the scorpion pincer, bending tests of cuticle samples confirm the macroscale isotropy of the strength, stiffness, and toughness.
33307544	9	45	theme	failure	1469:1475	arg1	analysis					1483:1490	Interlaminar failure (ILF) analysis	1456:1490	Interlaminar failure (ILF) analysis of the cuticle	1456:1505	Interlaminar failure (ILF) analysis of the cuticle leads to an estimation of the protein matrix shear strength, previously not measured.
33307544	2	46	theme	loads	432:436	arg1	magnitude					419:427	magnitude	419:427	magnitude	419:427	In these structures, the basic components are nanofibers or nanoplatelets, which are strong and stiff but anisotropic, whereas at the macrolevel, isotropy is preferred because the direction and magnitude of loads is unpredictable.
33307544	2	46	theme	loads	432:436	arg1	direction					405:413	direction	405:413	direction	405:413	In these structures, the basic components are nanofibers or nanoplatelets, which are strong and stiff but anisotropic, whereas at the macrolevel, isotropy is preferred because the direction and magnitude of loads is unpredictable.
33307544	10	47	with	composites	1672:1681	arg1	toughness					1718:1726	toughness	1718:1726	toughness	1718:1726	A similar structural approach can be adopted to the design of future synthetic composites with balanced strength, stiffness, toughness, and isotropy.
33307544	10	47	with	composites	1672:1681	arg1	isotropy					1733:1740	isotropy	1733:1740	isotropy	1733:1740	A similar structural approach can be adopted to the design of future synthetic composites with balanced strength, stiffness, toughness, and isotropy.
33307544	10	47	with	composites	1672:1681	arg1	stiffness					1707:1715	stiffness	1707:1715	stiffness	1707:1715	A similar structural approach can be adopted to the design of future synthetic composites with balanced strength, stiffness, toughness, and isotropy.
33307544	10	47	with	composites	1672:1681	arg1	strength					1697:1704	balanced strength	1688:1704	balanced strength	1688:1704	A similar structural approach can be adopted to the design of future synthetic composites with balanced strength, stiffness, toughness, and isotropy.
33307544	4	48	theme	fixed	676:680	arg1	tibia					669:673	the tibia	665:673	the tibia (fixed finger) of the scorpion pincer	665:711	Focusing on the tibia (fixed finger) of the scorpion pincer, bending tests of cuticle samples confirm the macroscale isotropy of the strength, stiffness, and toughness.
33307544	4	48	theme	fixed	676:680	arg1	finger					682:687	fixed finger	676:687	fixed finger	676:687	Focusing on the tibia (fixed finger) of the scorpion pincer, bending tests of cuticle samples confirm the macroscale isotropy of the strength, stiffness, and toughness.
33307544	8	49	theme	hierarchical	1382:1393	arg1	structure					1395:1403	the hierarchical structure	1378:1403	the hierarchical structure	1378:1403	These mechanisms, together with the hierarchical structure, are essential for achieving macro-scale isotropy.
33307544	8	50	theme	macro-scale	1434:1444	arg1	isotropy					1446:1453	macro-scale isotropy	1434:1453	macro-scale isotropy	1434:1453	These mechanisms, together with the hierarchical structure, are essential for achieving macro-scale isotropy.
33307544	2	51	theme	basic	250:254	arg1	nanofibers					271:280	nanofibers	271:280	nanofibers	271:280	In these structures, the basic components are nanofibers or nanoplatelets, which are strong and stiff but anisotropic, whereas at the macrolevel, isotropy is preferred because the direction and magnitude of loads is unpredictable.
33307544	2	51	theme	basic	250:254	arg1	components					256:265	the basic components	246:265	the basic components	246:265	In these structures, the basic components are nanofibers or nanoplatelets, which are strong and stiff but anisotropic, whereas at the macrolevel, isotropy is preferred because the direction and magnitude of loads is unpredictable.
33307544	9	52	theme	strength	1558:1565	arg1	estimation					1519:1528	an estimation	1516:1528	an estimation of the protein matrix shear strength	1516:1565	Interlaminar failure (ILF) analysis of the cuticle leads to an estimation of the protein matrix shear strength, previously not measured.
33307544	5	53	theme	multilayer	875:884	arg1	structure					896:904	an intricate multilayer laminated structure	862:904	an intricate multilayer laminated structure	862:904	Imaging analysis of the cuticle reveals an intricate multilayer laminated structure, with varying chitin-protein fiber orientations, arranged in eight hierarchical levels.
33307544	7	54	dep	analysis	1159:1166	arg1	laminate					1150:1157	laminate	1150:1157	laminate	1150:1157	Using laminate analysis to model the cuticle structure, we were able to correlate the nanostructure to the macro-mechanical properties, uncovering shear enhancing mechanisms at different length scales.
33307544	3	55	theme	current	638:644	arg1	study					646:650	the current study	634:650	the current study	634:650	The structural features and mechanisms, which drive the transition from anisotropy to isotropy across length scales, raise fundamental questions and are therefore the subject of the current study.
33307544	4	56	theme	samples	739:745	arg1	tests					722:726	bending tests	714:726	bending tests of cuticle samples	714:745	Focusing on the tibia (fixed finger) of the scorpion pincer, bending tests of cuticle samples confirm the macroscale isotropy of the strength, stiffness, and toughness.
33307544	3	57	theme	structural	460:469	arg1	features					471:478	The structural features	456:478	The structural features	456:478	The structural features and mechanisms, which drive the transition from anisotropy to isotropy across length scales, raise fundamental questions and are therefore the subject of the current study.
33307544	6	58	theme	intermediate	1079:1090	arg1	layer					1092:1096	a thick intermediate layer	1071:1096	a thick intermediate layer	1071:1096	We show that the cuticle flexural stiffness is increased by the existence of a thick intermediate layer, not seen before in the claws of crustaceans.
33307544	0	59	theme	cuticle	64:70	arg1	scales					34:39	length scales	27:39	length scales of the scorpion pincer cuticle	27:70	Structural analysis across length scales of the scorpion pincer cuticle.
33307544	3	60	theme	study	646:650	arg1	subject					623:629	the subject	619:629	the subject of the current study	619:650	The structural features and mechanisms, which drive the transition from anisotropy to isotropy across length scales, raise fundamental questions and are therefore the subject of the current study.
33307544	0	61	dep	analysis	11:18	arg1	scales					34:39	length scales	27:39	length scales of the scorpion pincer cuticle	27:70	Structural analysis across length scales of the scorpion pincer cuticle.
33307544	6	62	theme	flexural	1019:1026	arg1	stiffness					1028:1036	the cuticle flexural stiffness	1007:1036	the cuticle flexural stiffness	1007:1036	We show that the cuticle flexural stiffness is increased by the existence of a thick intermediate layer, not seen before in the claws of crustaceans.
33307544	5	63	theme	varying	912:918	arg1	orientations					941:952	varying chitin-protein fiber orientations	912:952	varying chitin-protein fiber orientations	912:952	Imaging analysis of the cuticle reveals an intricate multilayer laminated structure, with varying chitin-protein fiber orientations, arranged in eight hierarchical levels.
33307544	2	64	from	nanofibers	271:280	arg1	structures					234:243	these structures	228:243	these structures	228:243	In these structures, the basic components are nanofibers or nanoplatelets, which are strong and stiff but anisotropic, whereas at the macrolevel, isotropy is preferred because the direction and magnitude of loads is unpredictable.
33307544	0	65	theme	pincer	57:62	arg1	cuticle					64:70	the scorpion pincer cuticle	44:70	the scorpion pincer cuticle	44:70	Structural analysis across length scales of the scorpion pincer cuticle.
33307544	5	66	theme	hierarchical	973:984	arg1	levels					986:991	eight hierarchical levels	967:991	eight hierarchical levels	967:991	Imaging analysis of the cuticle reveals an intricate multilayer laminated structure, with varying chitin-protein fiber orientations, arranged in eight hierarchical levels.
33307544	10	67	theme	balanced	1688:1695	arg1	strength					1697:1704	balanced strength	1688:1704	balanced strength	1688:1704	A similar structural approach can be adopted to the design of future synthetic composites with balanced strength, stiffness, toughness, and isotropy.
31926236	4	0	theme	composite	516:524	arg1	film					526:529	the composite film	512:529	the composite film show reasonable mechanical properties, when the ratio of pullulan derivative:chitosan = 2:8 and the tensile strength was 21 MPa	512:657	Also, the addition of chitosan made the composite film show reasonable mechanical properties, when the ratio of pullulan derivative:chitosan = 2:8 and the tensile strength was 21 MPa.
31926236	0	1	theme	antimicrobial	94:106	arg1	applications					108:119	potential antimicrobial applications	84:119	potential antimicrobial applications	84:119	Preparation and characterization of pullulan derivative/chitosan composite film for potential antimicrobial applications.
31926236	4	2	theme	reasonable	536:545	arg1	properties					558:567	reasonable mechanical properties	536:567	reasonable mechanical properties	536:567	Also, the addition of chitosan made the composite film show reasonable mechanical properties, when the ratio of pullulan derivative:chitosan = 2:8 and the tensile strength was 21 MPa.
31926236	6	3	theme	packaging	850:858	arg1	film					860:863	a novel antibacterial packaging film	828:863	a novel antibacterial packaging film	828:863	This work is opening the door on preparing a novel antibacterial packaging film.
31926236	6	4	theme	novel	830:834	arg1	film					860:863	a novel antibacterial packaging film	828:863	a novel antibacterial packaging film	828:863	This work is opening the door on preparing a novel antibacterial packaging film.
31926236	4	5	theme	derivative	597:606	arg1	ratio					579:583	the ratio	575:583	the ratio of pullulan derivative	575:606	Also, the addition of chitosan made the composite film show reasonable mechanical properties, when the ratio of pullulan derivative:chitosan = 2:8 and the tensile strength was 21 MPa.
31926236	4	5	theme	derivative	597:606	arg1	21 MPa					652:657	21 MPa	652:657	21 MPa	652:657	Also, the addition of chitosan made the composite film show reasonable mechanical properties, when the ratio of pullulan derivative:chitosan = 2:8 and the tensile strength was 21 MPa.
31926236	4	5	theme	derivative	597:606	arg1	strength					639:646	the tensile strength	627:646	the tensile strength	627:646	Also, the addition of chitosan made the composite film show reasonable mechanical properties, when the ratio of pullulan derivative:chitosan = 2:8 and the tensile strength was 21 MPa.
31926236	4	6	theme	chitosan	498:505	arg1	addition					486:493	the addition	482:493	the addition of chitosan	482:505	Also, the addition of chitosan made the composite film show reasonable mechanical properties, when the ratio of pullulan derivative:chitosan = 2:8 and the tensile strength was 21 MPa.
31926236	3	7	theme	amino	365:369	arg1	groups					371:376	The active amino groups	354:376	The active amino groups on the pullulan derivative backbone and chitosan	354:425	The active amino groups on the pullulan derivative backbone and chitosan impart antimicrobial activity against S. aureus.
31926236	1	8	theme	shelf	200:204	arg1	life					206:209	shelf life	200:209	shelf life	200:209	Antimicrobial food-packaging films can improve the safety of food and prolong shelf life.
31926236	5	9	theme	barrier	765:771	arg1	properties					773:782	light barrier properties	759:782	light barrier properties	759:782	The composite film also behaved with the excellent film-forming property, Water vapor barrier, and light barrier properties.
31926236	5	10	theme	light	759:763	arg1	properties					773:782	light barrier properties	759:782	light barrier properties	759:782	The composite film also behaved with the excellent film-forming property, Water vapor barrier, and light barrier properties.
31926236	2	11	theme	derivative/chitosan	318:336	arg1	film					348:351	a pullulan derivative/chitosan composite film	307:351	a pullulan derivative/chitosan composite film	307:351	In the present work, we first prepared the pullulan derivative and then added chitosan to form a pullulan derivative/chitosan composite film.
31926236	2	12	theme	composite	338:346	arg1	film					348:351	a pullulan derivative/chitosan composite film	307:351	a pullulan derivative/chitosan composite film	307:351	In the present work, we first prepared the pullulan derivative and then added chitosan to form a pullulan derivative/chitosan composite film.
31926236	5	13	theme	composite	664:672	arg1	film					674:677	The composite film	660:677	The composite film	660:677	The composite film also behaved with the excellent film-forming property, Water vapor barrier, and light barrier properties.
31926236	1	14	theme	Antimicrobial	122:134	arg1	films					151:155	Antimicrobial food-packaging films	122:155	Antimicrobial food-packaging films	122:155	Antimicrobial food-packaging films can improve the safety of food and prolong shelf life.
31926236	3	15	theme	pullulan	385:392	arg1	backbone					405:412	the pullulan derivative backbone	381:412	the pullulan derivative backbone	381:412	The active amino groups on the pullulan derivative backbone and chitosan impart antimicrobial activity against S. aureus.
31926236	2	16	theme	pullulan	309:316	arg1	film					348:351	a pullulan derivative/chitosan composite film	307:351	a pullulan derivative/chitosan composite film	307:351	In the present work, we first prepared the pullulan derivative and then added chitosan to form a pullulan derivative/chitosan composite film.
31926236	3	17	theme	derivative	394:403	arg1	backbone					405:412	the pullulan derivative backbone	381:412	the pullulan derivative backbone	381:412	The active amino groups on the pullulan derivative backbone and chitosan impart antimicrobial activity against S. aureus.
31926236	4	18	theme	pullulan	588:595	arg1	derivative					597:606	pullulan derivative	588:606	pullulan derivative	588:606	Also, the addition of chitosan made the composite film show reasonable mechanical properties, when the ratio of pullulan derivative:chitosan = 2:8 and the tensile strength was 21 MPa.
31926236	5	19	theme	Water	734:738	arg1	barrier					746:752	Water vapor barrier	734:752	Water vapor barrier	734:752	The composite film also behaved with the excellent film-forming property, Water vapor barrier, and light barrier properties.
31926236	3	20	theme	antimicrobial	434:446	arg1	activity					448:455	antimicrobial activity	434:455	antimicrobial activity against S. aureus	434:473	The active amino groups on the pullulan derivative backbone and chitosan impart antimicrobial activity against S. aureus.
31926236	5	21	theme	vapor	740:744	arg1	barrier					746:752	Water vapor barrier	734:752	Water vapor barrier	734:752	The composite film also behaved with the excellent film-forming property, Water vapor barrier, and light barrier properties.
31926236	3	22	from	groups	371:376	arg1	backbone					405:412	the pullulan derivative backbone	381:412	the pullulan derivative backbone	381:412	The active amino groups on the pullulan derivative backbone and chitosan impart antimicrobial activity against S. aureus.
31926236	3	22	from	groups	371:376	arg1	chitosan					418:425	chitosan	418:425	chitosan	418:425	The active amino groups on the pullulan derivative backbone and chitosan impart antimicrobial activity against S. aureus.
31926236	0	23	theme	pullulan	36:43	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of pullulan derivative/chitosan composite film for potential antimicrobial applications.
31926236	0	23	theme	pullulan	36:43	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of pullulan derivative/chitosan composite film for potential antimicrobial applications.
31926236	4	24	theme	mechanical	547:556	arg1	properties					558:567	reasonable mechanical properties	536:567	reasonable mechanical properties	536:567	Also, the addition of chitosan made the composite film show reasonable mechanical properties, when the ratio of pullulan derivative:chitosan = 2:8 and the tensile strength was 21 MPa.
31926236	0	25	theme	composite	65:73	arg1	film					75:78	composite film	65:78	composite film for potential antimicrobial applications	65:119	Preparation and characterization of pullulan derivative/chitosan composite film for potential antimicrobial applications.
31926236	4	26	dep	film	526:529	arg1	show					531:534	show	531:534	show	531:534	Also, the addition of chitosan made the composite film show reasonable mechanical properties, when the ratio of pullulan derivative:chitosan = 2:8 and the tensile strength was 21 MPa.
31926236	5	27	theme	excellent	701:709	arg1	property					724:731	the excellent film-forming property	697:731	the excellent film-forming property	697:731	The composite film also behaved with the excellent film-forming property, Water vapor barrier, and light barrier properties.
31926236	5	28	theme	film-forming	711:722	arg1	property					724:731	the excellent film-forming property	697:731	the excellent film-forming property	697:731	The composite film also behaved with the excellent film-forming property, Water vapor barrier, and light barrier properties.
31926236	1	29	theme	food-packaging	136:149	arg1	films					151:155	Antimicrobial food-packaging films	122:155	Antimicrobial food-packaging films	122:155	Antimicrobial food-packaging films can improve the safety of food and prolong shelf life.
31926236	4	30	theme	tensile	631:637	arg1	21 MPa					652:657	21 MPa	652:657	21 MPa	652:657	Also, the addition of chitosan made the composite film show reasonable mechanical properties, when the ratio of pullulan derivative:chitosan = 2:8 and the tensile strength was 21 MPa.
31926236	4	30	theme	tensile	631:637	arg1	strength					639:646	the tensile strength	627:646	the tensile strength	627:646	Also, the addition of chitosan made the composite film show reasonable mechanical properties, when the ratio of pullulan derivative:chitosan = 2:8 and the tensile strength was 21 MPa.
31926236	2	31	theme	present	219:225	arg1	work					227:230	the present work	215:230	the present work	215:230	In the present work, we first prepared the pullulan derivative and then added chitosan to form a pullulan derivative/chitosan composite film.
31926236	0	32	theme	potential	84:92	arg1	applications					108:119	potential antimicrobial applications	84:119	potential antimicrobial applications	84:119	Preparation and characterization of pullulan derivative/chitosan composite film for potential antimicrobial applications.
31926236	6	33	theme	antibacterial	836:848	arg1	film					860:863	a novel antibacterial packaging film	828:863	a novel antibacterial packaging film	828:863	This work is opening the door on preparing a novel antibacterial packaging film.
31926236	3	34	theme	active	358:363	arg1	groups					371:376	The active amino groups	354:376	The active amino groups on the pullulan derivative backbone and chitosan	354:425	The active amino groups on the pullulan derivative backbone and chitosan impart antimicrobial activity against S. aureus.
31926236	2	35	theme	pullulan	255:262	arg1	derivative					264:273	the pullulan derivative	251:273	the pullulan derivative	251:273	In the present work, we first prepared the pullulan derivative and then added chitosan to form a pullulan derivative/chitosan composite film.
31926236	1	36	theme	food	183:186	arg1	safety					173:178	the safety	169:178	the safety of food	169:186	Antimicrobial food-packaging films can improve the safety of food and prolong shelf life.
33455763	7	0	theme	MelB	1358:1361	arg1	expression					1344:1353	the increased expression	1330:1353	the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase	1330:1430	Specially, the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase provided substrates for the EPS synthesis.
33455763	4	1	theme	molecular	763:771	arg1	components					780:789	decreased high molecular weight components	748:789	decreased high molecular weight components	748:789	The presence of Ca2+ also changed the monosaccharide composition of the EPS with decreased high molecular weight components and more content of rhamnose, though the functional groups of the polymer were not altered as revealed by Fourier transform infrared spectral analysis.
33455763	8	2	theme	significant	1517:1527	arg1	change					1529:1534	significant change	1517:1534	significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism	1517:1647	Meanwhile, metabolomics analysis revealed significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism.
33455763	11	3	theme	EPS-producing	2207:2219	arg1	potential					2221:2229	its EPS-producing potential	2203:2229	its EPS-producing potential for application in probiotic dairy products	2203:2273	This study provides a basis for understanding the overall change of metabolic pathways related to the EPS biosynthesis in L. plantarum K25 in response to Ca2+, facilitating exploitation of its EPS-producing potential for application in probiotic dairy products.
33455763	4	4	theme	EPS	739:741	arg1	composition					720:730	the monosaccharide composition	701:730	the monosaccharide composition of the EPS	701:741	The presence of Ca2+ also changed the monosaccharide composition of the EPS with decreased high molecular weight components and more content of rhamnose, though the functional groups of the polymer were not altered as revealed by Fourier transform infrared spectral analysis.
33455763	9	5	theme	5-phosphate	1732:1742	arg1	ribose					1744:1749	5-phosphate ribose	1732:1749	5-phosphate ribose	1732:1749	Among them the content of active small molecules such as polygalitol, lyxose, and 5-phosphate ribose increased, facilitating the EPS biosynthesis.
33455763	3	6	theme	EPS	610:612	arg1	yield					614:618	the EPS yield	606:618	the EPS yield	606:618	The results showed that addition of CaCl2 at 20 mg/L in a semi-defined medium did not affect the growth of strain K25, but it increased the EPS yield and changed the microstructure of the polymer.
33455763	1	7	from	component	263:271	arg1	milk					276:279	milk	276:279	milk	276:279	Exopolysaccharide (EPS)-producing lactic acid bacteria have been widely used in dairy products, but how calcium, the main metal ion component in milk, regulates the EPS biosynthesis in lactic acid bacteria is not clear.
33455763	8	8	theme	small	1543:1547	arg1	metabolites					1559:1569	the small molecular metabolites	1539:1569	the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism	1539:1647	Meanwhile, metabolomics analysis revealed significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism.
33455763	1	9	theme	acid	172:175	arg1	bacteria					177:184	Exopolysaccharide (EPS)-producing lactic acid bacteria	131:184	Exopolysaccharide (EPS)-producing lactic acid bacteria	131:184	Exopolysaccharide (EPS)-producing lactic acid bacteria have been widely used in dairy products, but how calcium, the main metal ion component in milk, regulates the EPS biosynthesis in lactic acid bacteria is not clear.
33455763	4	10	theme	decreased	748:756	arg1	components					780:789	decreased high molecular weight components	748:789	decreased high molecular weight components	748:789	The presence of Ca2+ also changed the monosaccharide composition of the EPS with decreased high molecular weight components and more content of rhamnose, though the functional groups of the polymer were not altered as revealed by Fourier transform infrared spectral analysis.
33455763	8	11	from	cycle	1593:1597	arg1	change					1529:1534	significant change	1517:1534	significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism	1517:1647	Meanwhile, metabolomics analysis revealed significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism.
33455763	4	12	dep	transform	905:913	arg1	infrared					915:922	infrared	915:922	transform infrared spectral analysis	905:940	The presence of Ca2+ also changed the monosaccharide composition of the EPS with decreased high molecular weight components and more content of rhamnose, though the functional groups of the polymer were not altered as revealed by Fourier transform infrared spectral analysis.
33455763	3	13	theme	CaCl2	506:510	arg1	addition					494:501	addition	494:501	addition of CaCl2 at 20 mg/L in a semi-defined medium	494:546	The results showed that addition of CaCl2 at 20 mg/L in a semi-defined medium did not affect the growth of strain K25, but it increased the EPS yield and changed the microstructure of the polymer.
33455763	8	14	theme	metabolites	1559:1569	arg1	change					1529:1534	significant change	1517:1534	significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism	1517:1647	Meanwhile, metabolomics analysis revealed significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism.
33455763	7	15	theme	EPS	1460:1462	arg1	synthesis					1464:1472	the EPS synthesis	1456:1472	the EPS synthesis	1456:1472	Specially, the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase provided substrates for the EPS synthesis.
33455763	6	16	theme	carbon	1212:1217	arg1	transport					1219:1227	carbon transport	1212:1227	carbon transport	1212:1227	Proteomics analysis showed that Ca2+ upregulated most of the proteins related to carbon transport and metabolism, fatty acid synthesis, amino acid synthesis, ion transport, UMP synthesis.
33455763	8	17	theme	tricarboxylic	1574:1586	arg1	cycle					1593:1597	tricarboxylic acid cycle	1574:1597	tricarboxylic acid cycle	1574:1597	Meanwhile, metabolomics analysis revealed significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism.
33455763	9	18	theme	EPS	1779:1781	arg1	biosynthesis					1783:1794	the EPS biosynthesis	1775:1794	the EPS biosynthesis	1775:1794	Among them the content of active small molecules such as polygalitol, lyxose, and 5-phosphate ribose increased, facilitating the EPS biosynthesis.
33455763	10	19	theme	signaling	1830:1838	arg1	pathway					1840:1846	HipB signaling pathway	1825:1846	HipB signaling pathway	1825:1846	Furthermore, Ca2+ activated HipB signaling pathway to inhibit the expression of manipulator repressor such as ArsR, LytR/AlgR, IscR, and RafR, and activated the expression of GntR to regulate the EPS synthesis genes.
33455763	6	20	theme	related	1201:1207	arg1	proteins					1192:1199	the proteins	1188:1199	the proteins related to carbon transport and metabolism, fatty acid synthesis, amino acid synthesis, ion transport, UMP synthesis	1188:1316	Proteomics analysis showed that Ca2+ upregulated most of the proteins related to carbon transport and metabolism, fatty acid synthesis, amino acid synthesis, ion transport, UMP synthesis.
33455763	3	21	theme	polymer	658:664	arg1	microstructure					636:649	the microstructure	632:649	the microstructure of the polymer	632:664	The results showed that addition of CaCl2 at 20 mg/L in a semi-defined medium did not affect the growth of strain K25, but it increased the EPS yield and changed the microstructure of the polymer.
33455763	8	22	theme	glucose	1600:1606	arg1	metabolism					1608:1617	glucose metabolism	1600:1617	glucose metabolism	1600:1617	Meanwhile, metabolomics analysis revealed significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism.
33455763	10	23	theme	GntR	1972:1975	arg1	expression					1958:1967	the expression	1954:1967	the expression of GntR	1954:1975	Furthermore, Ca2+ activated HipB signaling pathway to inhibit the expression of manipulator repressor such as ArsR, LytR/AlgR, IscR, and RafR, and activated the expression of GntR to regulate the EPS synthesis genes.
33455763	11	24	dep	Ca2+	2168:2171	arg1	response					2156:2163	response	2156:2163	response	2156:2163	This study provides a basis for understanding the overall change of metabolic pathways related to the EPS biosynthesis in L. plantarum K25 in response to Ca2+, facilitating exploitation of its EPS-producing potential for application in probiotic dairy products.
33455763	1	25	theme	Exopolysaccharide	131:147	arg1	bacteria					177:184	Exopolysaccharide (EPS)-producing lactic acid bacteria	131:184	Exopolysaccharide (EPS)-producing lactic acid bacteria	131:184	Exopolysaccharide (EPS)-producing lactic acid bacteria have been widely used in dairy products, but how calcium, the main metal ion component in milk, regulates the EPS biosynthesis in lactic acid bacteria is not clear.
33455763	7	26	theme	DhaK	1415:1418	arg1	expression					1344:1353	the increased expression	1330:1353	the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase	1330:1430	Specially, the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase provided substrates for the EPS synthesis.
33455763	2	27	theme	Lactobacillus	429:441	arg1	K25					453:455	the probiotic Lactobacillus plantarum K25	415:455	the probiotic Lactobacillus plantarum K25	415:455	In this study, the effect of Ca2+ on the biosynthesis of EPS in the probiotic Lactobacillus plantarum K25 was studied.
33455763	0	28	theme	probiotic	59:67	arg1	K25					93:95	probiotic Lactobacillus plantarum K25	59:95	probiotic Lactobacillus plantarum K25	59:95	Calcium (Ca2+)-regulated exopolysaccharide biosynthesis in probiotic Lactobacillus plantarum K25 as analyzed by an omics approach.
33455763	5	29	theme	cps	1010:1012	arg1	rfbD					1078:1081	rfbD	1078:1081	rfbD	1078:1081	These were further confirmed by analysis of the mRNA expression of cps genes, 9 of which were upregulated by Ca2+, including cps4F and rfbD associated with EPS biosynthesis with rhamnose.
33455763	5	29	theme	cps	1010:1012	arg1	genes					1014:1018	cps genes	1010:1018	cps genes	1010:1018	These were further confirmed by analysis of the mRNA expression of cps genes, 9 of which were upregulated by Ca2+, including cps4F and rfbD associated with EPS biosynthesis with rhamnose.
33455763	5	29	theme	cps	1010:1012	arg1	cps4F					1068:1072	cps4F	1068:1072	cps4F	1068:1072	These were further confirmed by analysis of the mRNA expression of cps genes, 9 of which were upregulated by Ca2+, including cps4F and rfbD associated with EPS biosynthesis with rhamnose.
33455763	3	30	theme	semi-defined	528:539	arg1	medium					541:546	a semi-defined medium	526:546	a semi-defined medium	526:546	The results showed that addition of CaCl2 at 20 mg/L in a semi-defined medium did not affect the growth of strain K25, but it increased the EPS yield and changed the microstructure of the polymer.
33455763	8	31	theme	acid	1633:1636	arg1	metabolism					1638:1647	propionic acid metabolism	1623:1647	propionic acid metabolism	1623:1647	Meanwhile, metabolomics analysis revealed significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism.
33455763	7	32	theme	MalFGK	1407:1412	arg1	expression					1344:1353	the increased expression	1330:1353	the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase	1330:1430	Specially, the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase provided substrates for the EPS synthesis.
33455763	2	33	from	effect	370:375	arg1	biosynthesis					392:403	the biosynthesis	388:403	the biosynthesis of EPS in the probiotic Lactobacillus plantarum K25	388:455	In this study, the effect of Ca2+ on the biosynthesis of EPS in the probiotic Lactobacillus plantarum K25 was studied.
33455763	10	34	theme	EPS	1993:1995	arg1	genes					2007:2011	the EPS synthesis genes	1989:2011	the EPS synthesis genes	1989:2011	Furthermore, Ca2+ activated HipB signaling pathway to inhibit the expression of manipulator repressor such as ArsR, LytR/AlgR, IscR, and RafR, and activated the expression of GntR to regulate the EPS synthesis genes.
33455763	10	35	theme	repressor	1889:1897	arg1	expression					1863:1872	the expression	1859:1872	the expression of manipulator repressor such as ArsR, LytR/AlgR, IscR, and RafR	1859:1937	Furthermore, Ca2+ activated HipB signaling pathway to inhibit the expression of manipulator repressor such as ArsR, LytR/AlgR, IscR, and RafR, and activated the expression of GntR to regulate the EPS synthesis genes.
33455763	0	36	theme	plantarum	83:91	arg1	K25					93:95	probiotic Lactobacillus plantarum K25	59:95	probiotic Lactobacillus plantarum K25	59:95	Calcium (Ca2+)-regulated exopolysaccharide biosynthesis in probiotic Lactobacillus plantarum K25 as analyzed by an omics approach.
33455763	1	37	theme	-producing	154:163	arg1	bacteria					177:184	Exopolysaccharide (EPS)-producing lactic acid bacteria	131:184	Exopolysaccharide (EPS)-producing lactic acid bacteria	131:184	Exopolysaccharide (EPS)-producing lactic acid bacteria have been widely used in dairy products, but how calcium, the main metal ion component in milk, regulates the EPS biosynthesis in lactic acid bacteria is not clear.
33455763	7	38	theme	GH1	1402:1404	arg1	expression					1344:1353	the increased expression	1330:1353	the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase	1330:1430	Specially, the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase provided substrates for the EPS synthesis.
33455763	1	39	theme	lactic	316:321	arg1	bacteria					328:335	lactic acid bacteria	316:335	lactic acid bacteria	316:335	Exopolysaccharide (EPS)-producing lactic acid bacteria have been widely used in dairy products, but how calcium, the main metal ion component in milk, regulates the EPS biosynthesis in lactic acid bacteria is not clear.
33455763	7	40	theme	Bgl	1397:1399	arg1	expression					1344:1353	the increased expression	1330:1353	the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase	1330:1430	Specially, the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase provided substrates for the EPS synthesis.
33455763	5	41	theme	expression	996:1005	arg1	analysis					975:982	analysis	975:982	analysis of the mRNA expression of cps genes, 9 of which were upregulated by Ca2+, including cps4F and rfbD associated with EPS biosynthesis with rhamnose	975:1128	These were further confirmed by analysis of the mRNA expression of cps genes, 9 of which were upregulated by Ca2+, including cps4F and rfbD associated with EPS biosynthesis with rhamnose.
33455763	0	42	theme	exopolysaccharide	25:41	arg1	biosynthesis					43:54	exopolysaccharide biosynthesis	25:54	exopolysaccharide biosynthesis	25:54	Calcium (Ca2+)-regulated exopolysaccharide biosynthesis in probiotic Lactobacillus plantarum K25 as analyzed by an omics approach.
33455763	0	43	from	-regulated	14:23	arg1	K25					93:95	probiotic Lactobacillus plantarum K25	59:95	probiotic Lactobacillus plantarum K25	59:95	Calcium (Ca2+)-regulated exopolysaccharide biosynthesis in probiotic Lactobacillus plantarum K25 as analyzed by an omics approach.
33455763	0	44	theme	Lactobacillus	69:81	arg1	K25					93:95	probiotic Lactobacillus plantarum K25	59:95	probiotic Lactobacillus plantarum K25	59:95	Calcium (Ca2+)-regulated exopolysaccharide biosynthesis in probiotic Lactobacillus plantarum K25 as analyzed by an omics approach.
33455763	6	45	theme	UMP	1304:1306	arg1	synthesis					1308:1316	UMP synthesis	1304:1316	UMP synthesis	1304:1316	Proteomics analysis showed that Ca2+ upregulated most of the proteins related to carbon transport and metabolism, fatty acid synthesis, amino acid synthesis, ion transport, UMP synthesis.
33455763	1	46	theme	main	248:251	arg1	calcium					235:241	calcium	235:241	calcium	235:241	Exopolysaccharide (EPS)-producing lactic acid bacteria have been widely used in dairy products, but how calcium, the main metal ion component in milk, regulates the EPS biosynthesis in lactic acid bacteria is not clear.
33455763	1	46	theme	main	248:251	arg1	component					263:271	the main metal ion component	244:271	the main metal ion component in milk	244:279	Exopolysaccharide (EPS)-producing lactic acid bacteria have been widely used in dairy products, but how calcium, the main metal ion component in milk, regulates the EPS biosynthesis in lactic acid bacteria is not clear.
33455763	1	47	theme	ion	259:261	arg1	calcium					235:241	calcium	235:241	calcium	235:241	Exopolysaccharide (EPS)-producing lactic acid bacteria have been widely used in dairy products, but how calcium, the main metal ion component in milk, regulates the EPS biosynthesis in lactic acid bacteria is not clear.
33455763	1	47	theme	ion	259:261	arg1	component					263:271	the main metal ion component	244:271	the main metal ion component in milk	244:279	Exopolysaccharide (EPS)-producing lactic acid bacteria have been widely used in dairy products, but how calcium, the main metal ion component in milk, regulates the EPS biosynthesis in lactic acid bacteria is not clear.
33455763	2	48	theme	EPS	408:410	arg1	biosynthesis					392:403	the biosynthesis	388:403	the biosynthesis of EPS in the probiotic Lactobacillus plantarum K25	388:455	In this study, the effect of Ca2+ on the biosynthesis of EPS in the probiotic Lactobacillus plantarum K25 was studied.
33455763	11	49	theme	metabolic	2082:2090	arg1	pathways					2092:2099	metabolic pathways	2082:2099	metabolic pathways related to the EPS biosynthesis in L. plantarum K25 in response to Ca2+	2082:2171	This study provides a basis for understanding the overall change of metabolic pathways related to the EPS biosynthesis in L. plantarum K25 in response to Ca2+, facilitating exploitation of its EPS-producing potential for application in probiotic dairy products.
33455763	4	50	theme	monosaccharide	705:718	arg1	composition					720:730	the monosaccharide composition	701:730	the monosaccharide composition of the EPS	701:741	The presence of Ca2+ also changed the monosaccharide composition of the EPS with decreased high molecular weight components and more content of rhamnose, though the functional groups of the polymer were not altered as revealed by Fourier transform infrared spectral analysis.
33455763	11	51	from	application	2235:2245	arg1	products					2266:2273	probiotic dairy products	2250:2273	probiotic dairy products	2250:2273	This study provides a basis for understanding the overall change of metabolic pathways related to the EPS biosynthesis in L. plantarum K25 in response to Ca2+, facilitating exploitation of its EPS-producing potential for application in probiotic dairy products.
33455763	4	52	theme	polymer	857:863	arg1	groups					843:848	the functional groups	828:848	the functional groups of the polymer	828:863	The presence of Ca2+ also changed the monosaccharide composition of the EPS with decreased high molecular weight components and more content of rhamnose, though the functional groups of the polymer were not altered as revealed by Fourier transform infrared spectral analysis.
33455763	4	52	theme	polymer	857:863	arg1	polymer					857:863	the polymer	853:863	the polymer	853:863	The presence of Ca2+ also changed the monosaccharide composition of the EPS with decreased high molecular weight components and more content of rhamnose, though the functional groups of the polymer were not altered as revealed by Fourier transform infrared spectral analysis.
33455763	6	53	theme	acid	1273:1276	arg1	synthesis					1278:1286	amino acid synthesis	1267:1286	amino acid synthesis	1267:1286	Proteomics analysis showed that Ca2+ upregulated most of the proteins related to carbon transport and metabolism, fatty acid synthesis, amino acid synthesis, ion transport, UMP synthesis.
33455763	6	54	theme	Proteomics	1131:1140	arg1	analysis					1142:1149	Proteomics analysis	1131:1149	Proteomics analysis	1131:1149	Proteomics analysis showed that Ca2+ upregulated most of the proteins related to carbon transport and metabolism, fatty acid synthesis, amino acid synthesis, ion transport, UMP synthesis.
33455763	9	55	theme	small	1683:1687	arg1	ribose					1744:1749	5-phosphate ribose	1732:1749	5-phosphate ribose	1732:1749	Among them the content of active small molecules such as polygalitol, lyxose, and 5-phosphate ribose increased, facilitating the EPS biosynthesis.
33455763	9	55	theme	small	1683:1687	arg1	lyxose					1720:1725	lyxose	1720:1725	lyxose	1720:1725	Among them the content of active small molecules such as polygalitol, lyxose, and 5-phosphate ribose increased, facilitating the EPS biosynthesis.
33455763	9	55	theme	small	1683:1687	arg1	molecules					1689:1697	active small molecules	1676:1697	active small molecules such as polygalitol, lyxose, and 5-phosphate ribose	1676:1749	Among them the content of active small molecules such as polygalitol, lyxose, and 5-phosphate ribose increased, facilitating the EPS biosynthesis.
33455763	9	55	theme	small	1683:1687	arg1	polygalitol					1707:1717	polygalitol	1707:1717	polygalitol	1707:1717	Among them the content of active small molecules such as polygalitol, lyxose, and 5-phosphate ribose increased, facilitating the EPS biosynthesis.
33455763	4	56	theme	Ca2+	683:686	arg1	presence					671:678	The presence	667:678	The presence of Ca2+	667:686	The presence of Ca2+ also changed the monosaccharide composition of the EPS with decreased high molecular weight components and more content of rhamnose, though the functional groups of the polymer were not altered as revealed by Fourier transform infrared spectral analysis.
33455763	11	57	theme	probiotic	2250:2258	arg1	products					2266:2273	probiotic dairy products	2250:2273	probiotic dairy products	2250:2273	This study provides a basis for understanding the overall change of metabolic pathways related to the EPS biosynthesis in L. plantarum K25 in response to Ca2+, facilitating exploitation of its EPS-producing potential for application in probiotic dairy products.
33455763	1	58	theme	dairy	211:215	arg1	products					217:224	dairy products	211:224	dairy products	211:224	Exopolysaccharide (EPS)-producing lactic acid bacteria have been widely used in dairy products, but how calcium, the main metal ion component in milk, regulates the EPS biosynthesis in lactic acid bacteria is not clear.
33455763	11	59	theme	EPS	2116:2118	arg1	biosynthesis					2120:2131	the EPS biosynthesis	2112:2131	the EPS biosynthesis in L. plantarum K25 in response to Ca2+	2112:2171	This study provides a basis for understanding the overall change of metabolic pathways related to the EPS biosynthesis in L. plantarum K25 in response to Ca2+, facilitating exploitation of its EPS-producing potential for application in probiotic dairy products.
33455763	3	60	theme	strain	577:582	arg1	growth					567:572	the growth	563:572	the growth of strain K25	563:586	The results showed that addition of CaCl2 at 20 mg/L in a semi-defined medium did not affect the growth of strain K25, but it increased the EPS yield and changed the microstructure of the polymer.
33455763	8	61	from	metabolites	1559:1569	arg1	metabolism					1638:1647	propionic acid metabolism	1623:1647	propionic acid metabolism	1623:1647	Meanwhile, metabolomics analysis revealed significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism.
33455763	8	61	from	metabolites	1559:1569	arg1	cycle					1593:1597	tricarboxylic acid cycle	1574:1597	tricarboxylic acid cycle	1574:1597	Meanwhile, metabolomics analysis revealed significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism.
33455763	8	61	from	metabolites	1559:1569	arg1	metabolism					1608:1617	glucose metabolism	1600:1617	glucose metabolism	1600:1617	Meanwhile, metabolomics analysis revealed significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism.
33455763	4	62	dep	Fourier	897:903	arg1	transform					905:913	transform	905:913	transform infrared spectral analysis	905:940	The presence of Ca2+ also changed the monosaccharide composition of the EPS with decreased high molecular weight components and more content of rhamnose, though the functional groups of the polymer were not altered as revealed by Fourier transform infrared spectral analysis.
33455763	8	63	theme	metabolomics	1486:1497	arg1	Meanwhile					1475:1483	Meanwhile	1475:1483	Meanwhile	1475:1483	Meanwhile, metabolomics analysis revealed significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism.
33455763	8	63	theme	metabolomics	1486:1497	arg1	analysis					1499:1506	metabolomics analysis	1486:1506	metabolomics analysis	1486:1506	Meanwhile, metabolomics analysis revealed significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism.
33455763	5	64	theme	genes	1014:1018	arg1	expression					996:1005	the mRNA expression	987:1005	the mRNA expression of cps genes, 9 of which were upregulated by Ca2+, including cps4F and rfbD associated with EPS biosynthesis with rhamnose	987:1128	These were further confirmed by analysis of the mRNA expression of cps genes, 9 of which were upregulated by Ca2+, including cps4F and rfbD associated with EPS biosynthesis with rhamnose.
33455763	5	64	theme	genes	1014:1018	arg1	genes					1014:1018	cps genes	1010:1018	cps genes	1010:1018	These were further confirmed by analysis of the mRNA expression of cps genes, 9 of which were upregulated by Ca2+, including cps4F and rfbD associated with EPS biosynthesis with rhamnose.
33455763	5	64	theme	genes	1014:1018	arg1	cps4F					1068:1072	cps4F	1068:1072	cps4F	1068:1072	These were further confirmed by analysis of the mRNA expression of cps genes, 9 of which were upregulated by Ca2+, including cps4F and rfbD associated with EPS biosynthesis with rhamnose.
33455763	5	64	theme	genes	1014:1018	arg1	rfbD					1078:1081	rfbD	1078:1081	rfbD	1078:1081	These were further confirmed by analysis of the mRNA expression of cps genes, 9 of which were upregulated by Ca2+, including cps4F and rfbD associated with EPS biosynthesis with rhamnose.
33455763	5	64	theme	genes	1014:1018	arg1	9					1021:1021	9	1021:1021	9	1021:1021	These were further confirmed by analysis of the mRNA expression of cps genes, 9 of which were upregulated by Ca2+, including cps4F and rfbD associated with EPS biosynthesis with rhamnose.
33455763	7	65	theme	increased	1334:1342	arg1	expression					1344:1353	the increased expression	1330:1353	the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase	1330:1430	Specially, the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase provided substrates for the EPS synthesis.
33455763	4	66	theme	high	758:761	arg1	components					780:789	decreased high molecular weight components	748:789	decreased high molecular weight components	748:789	The presence of Ca2+ also changed the monosaccharide composition of the EPS with decreased high molecular weight components and more content of rhamnose, though the functional groups of the polymer were not altered as revealed by Fourier transform infrared spectral analysis.
33455763	11	67	theme	plantarum	2139:2147	arg1	K25					2149:2151	L. plantarum K25	2136:2151	L. plantarum K25	2136:2151	This study provides a basis for understanding the overall change of metabolic pathways related to the EPS biosynthesis in L. plantarum K25 in response to Ca2+, facilitating exploitation of its EPS-producing potential for application in probiotic dairy products.
33455763	11	68	theme	dairy	2260:2264	arg1	products					2266:2273	probiotic dairy products	2250:2273	probiotic dairy products	2250:2273	This study provides a basis for understanding the overall change of metabolic pathways related to the EPS biosynthesis in L. plantarum K25 in response to Ca2+, facilitating exploitation of its EPS-producing potential for application in probiotic dairy products.
33455763	4	69	theme	weight	773:778	arg1	components					780:789	decreased high molecular weight components	748:789	decreased high molecular weight components	748:789	The presence of Ca2+ also changed the monosaccharide composition of the EPS with decreased high molecular weight components and more content of rhamnose, though the functional groups of the polymer were not altered as revealed by Fourier transform infrared spectral analysis.
33455763	1	70	theme	lactic	165:170	arg1	bacteria					177:184	Exopolysaccharide (EPS)-producing lactic acid bacteria	131:184	Exopolysaccharide (EPS)-producing lactic acid bacteria	131:184	Exopolysaccharide (EPS)-producing lactic acid bacteria have been widely used in dairy products, but how calcium, the main metal ion component in milk, regulates the EPS biosynthesis in lactic acid bacteria is not clear.
33455763	11	71	theme	overall	2064:2070	arg1	change					2072:2077	the overall change	2060:2077	the overall change of metabolic pathways related to the EPS biosynthesis in L. plantarum K25 in response to Ca2+	2060:2171	This study provides a basis for understanding the overall change of metabolic pathways related to the EPS biosynthesis in L. plantarum K25 in response to Ca2+, facilitating exploitation of its EPS-producing potential for application in probiotic dairy products.
33455763	6	72	theme	acid	1251:1254	arg1	synthesis					1256:1264	fatty acid synthesis	1245:1264	fatty acid synthesis	1245:1264	Proteomics analysis showed that Ca2+ upregulated most of the proteins related to carbon transport and metabolism, fatty acid synthesis, amino acid synthesis, ion transport, UMP synthesis.
33455763	8	73	theme	molecular	1549:1557	arg1	metabolites					1559:1569	the small molecular metabolites	1539:1569	the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism	1539:1647	Meanwhile, metabolomics analysis revealed significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism.
33455763	11	74	from	biosynthesis	2120:2131	arg1	K25					2149:2151	L. plantarum K25	2136:2151	L. plantarum K25	2136:2151	This study provides a basis for understanding the overall change of metabolic pathways related to the EPS biosynthesis in L. plantarum K25 in response to Ca2+, facilitating exploitation of its EPS-producing potential for application in probiotic dairy products.
33455763	4	75	theme	rhamnose	811:818	arg1	content					800:806	more content	795:806	more content of rhamnose	795:818	The presence of Ca2+ also changed the monosaccharide composition of the EPS with decreased high molecular weight components and more content of rhamnose, though the functional groups of the polymer were not altered as revealed by Fourier transform infrared spectral analysis.
33455763	4	75	theme	rhamnose	811:818	arg1	components					780:789	decreased high molecular weight components	748:789	decreased high molecular weight components	748:789	The presence of Ca2+ also changed the monosaccharide composition of the EPS with decreased high molecular weight components and more content of rhamnose, though the functional groups of the polymer were not altered as revealed by Fourier transform infrared spectral analysis.
33455763	0	76	from	K25	93:95	arg1	-regulated					14:23	-regulated	14:23	-regulated	14:23	Calcium (Ca2+)-regulated exopolysaccharide biosynthesis in probiotic Lactobacillus plantarum K25 as analyzed by an omics approach.
33455763	11	77	theme	pathways	2092:2099	arg1	change					2072:2077	the overall change	2060:2077	the overall change of metabolic pathways related to the EPS biosynthesis in L. plantarum K25 in response to Ca2+	2060:2171	This study provides a basis for understanding the overall change of metabolic pathways related to the EPS biosynthesis in L. plantarum K25 in response to Ca2+, facilitating exploitation of its EPS-producing potential for application in probiotic dairy products.
33455763	7	78	theme	FBPase	1425:1430	arg1	expression					1344:1353	the increased expression	1330:1353	the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase	1330:1430	Specially, the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase provided substrates for the EPS synthesis.
33455763	1	79	used	used	203:206	arg2	bacteria					177:184	Exopolysaccharide (EPS)-producing lactic acid bacteria	131:184	Exopolysaccharide (EPS)-producing lactic acid bacteria	131:184	Exopolysaccharide (EPS)-producing lactic acid bacteria have been widely used in dairy products, but how calcium, the main metal ion component in milk, regulates the EPS biosynthesis in lactic acid bacteria is not clear.
33455763	8	80	from	metabolism	1608:1617	arg1	change					1529:1534	significant change	1517:1534	significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism	1517:1647	Meanwhile, metabolomics analysis revealed significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism.
33455763	10	81	theme	HipB	1825:1828	arg1	pathway					1840:1846	HipB signaling pathway	1825:1846	HipB signaling pathway	1825:1846	Furthermore, Ca2+ activated HipB signaling pathway to inhibit the expression of manipulator repressor such as ArsR, LytR/AlgR, IscR, and RafR, and activated the expression of GntR to regulate the EPS synthesis genes.
33455763	8	82	theme	acid	1588:1591	arg1	cycle					1593:1597	tricarboxylic acid cycle	1574:1597	tricarboxylic acid cycle	1574:1597	Meanwhile, metabolomics analysis revealed significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism.
33455763	11	83	theme	potential	2221:2229	arg1	exploitation					2187:2198	exploitation	2187:2198	exploitation of its EPS-producing potential for application in probiotic dairy products	2187:2273	This study provides a basis for understanding the overall change of metabolic pathways related to the EPS biosynthesis in L. plantarum K25 in response to Ca2+, facilitating exploitation of its EPS-producing potential for application in probiotic dairy products.
33455763	1	84	from	biosynthesis	300:311	arg1	bacteria					328:335	lactic acid bacteria	316:335	lactic acid bacteria	316:335	Exopolysaccharide (EPS)-producing lactic acid bacteria have been widely used in dairy products, but how calcium, the main metal ion component in milk, regulates the EPS biosynthesis in lactic acid bacteria is not clear.
33455763	8	85	from	metabolism	1638:1647	arg1	change					1529:1534	significant change	1517:1534	significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism	1517:1647	Meanwhile, metabolomics analysis revealed significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism.
33455763	6	86	theme	amino	1267:1271	arg1	synthesis					1278:1286	amino acid synthesis	1267:1286	amino acid synthesis	1267:1286	Proteomics analysis showed that Ca2+ upregulated most of the proteins related to carbon transport and metabolism, fatty acid synthesis, amino acid synthesis, ion transport, UMP synthesis.
33455763	11	87	theme	L.	2136:2137	arg1	K25					2149:2151	L. plantarum K25	2136:2151	L. plantarum K25	2136:2151	This study provides a basis for understanding the overall change of metabolic pathways related to the EPS biosynthesis in L. plantarum K25 in response to Ca2+, facilitating exploitation of its EPS-producing potential for application in probiotic dairy products.
33455763	2	88	theme	probiotic	419:427	arg1	K25					453:455	the probiotic Lactobacillus plantarum K25	415:455	the probiotic Lactobacillus plantarum K25	415:455	In this study, the effect of Ca2+ on the biosynthesis of EPS in the probiotic Lactobacillus plantarum K25 was studied.
33455763	10	89	theme	synthesis	1997:2005	arg1	genes					2007:2011	the EPS synthesis genes	1989:2011	the EPS synthesis genes	1989:2011	Furthermore, Ca2+ activated HipB signaling pathway to inhibit the expression of manipulator repressor such as ArsR, LytR/AlgR, IscR, and RafR, and activated the expression of GntR to regulate the EPS synthesis genes.
33455763	1	90	theme	EPS	296:298	arg1	biosynthesis					300:311	the EPS biosynthesis	292:311	the EPS biosynthesis in lactic acid bacteria	292:335	Exopolysaccharide (EPS)-producing lactic acid bacteria have been widely used in dairy products, but how calcium, the main metal ion component in milk, regulates the EPS biosynthesis in lactic acid bacteria is not clear.
33455763	8	91	theme	propionic	1623:1631	arg1	acid					1633:1636	propionic acid	1623:1636	propionic acid metabolism	1623:1647	Meanwhile, metabolomics analysis revealed significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism.
33455763	0	92	theme	omics	115:119	arg1	approach					121:128	an omics approach	112:128	an omics approach	112:128	Calcium (Ca2+)-regulated exopolysaccharide biosynthesis in probiotic Lactobacillus plantarum K25 as analyzed by an omics approach.
33455763	10	93	theme	manipulator	1877:1887	arg1	repressor					1889:1897	manipulator repressor	1877:1897	manipulator repressor	1877:1897	Furthermore, Ca2+ activated HipB signaling pathway to inhibit the expression of manipulator repressor such as ArsR, LytR/AlgR, IscR, and RafR, and activated the expression of GntR to regulate the EPS synthesis genes.
33455763	6	94	theme	fatty	1245:1249	arg1	synthesis					1256:1264	fatty acid synthesis	1245:1264	fatty acid synthesis	1245:1264	Proteomics analysis showed that Ca2+ upregulated most of the proteins related to carbon transport and metabolism, fatty acid synthesis, amino acid synthesis, ion transport, UMP synthesis.
33455763	4	95	theme	functional	832:841	arg1	groups					843:848	the functional groups	828:848	the functional groups of the polymer	828:863	The presence of Ca2+ also changed the monosaccharide composition of the EPS with decreased high molecular weight components and more content of rhamnose, though the functional groups of the polymer were not altered as revealed by Fourier transform infrared spectral analysis.
33455763	4	95	theme	functional	832:841	arg1	polymer					857:863	the polymer	853:863	the polymer	853:863	The presence of Ca2+ also changed the monosaccharide composition of the EPS with decreased high molecular weight components and more content of rhamnose, though the functional groups of the polymer were not altered as revealed by Fourier transform infrared spectral analysis.
33455763	2	96	from	biosynthesis	392:403	arg1	K25					453:455	the probiotic Lactobacillus plantarum K25	415:455	the probiotic Lactobacillus plantarum K25	415:455	In this study, the effect of Ca2+ on the biosynthesis of EPS in the probiotic Lactobacillus plantarum K25 was studied.
33455763	1	97	theme	acid	323:326	arg1	bacteria					328:335	lactic acid bacteria	316:335	lactic acid bacteria	316:335	Exopolysaccharide (EPS)-producing lactic acid bacteria have been widely used in dairy products, but how calcium, the main metal ion component in milk, regulates the EPS biosynthesis in lactic acid bacteria is not clear.
33455763	2	98	theme	plantarum	443:451	arg1	K25					453:455	the probiotic Lactobacillus plantarum K25	415:455	the probiotic Lactobacillus plantarum K25	415:455	In this study, the effect of Ca2+ on the biosynthesis of EPS in the probiotic Lactobacillus plantarum K25 was studied.
33455763	5	99	theme	mRNA	991:994	arg1	expression					996:1005	the mRNA expression	987:1005	the mRNA expression of cps genes, 9 of which were upregulated by Ca2+, including cps4F and rfbD associated with EPS biosynthesis with rhamnose	987:1128	These were further confirmed by analysis of the mRNA expression of cps genes, 9 of which were upregulated by Ca2+, including cps4F and rfbD associated with EPS biosynthesis with rhamnose.
33455763	2	100	theme	Ca2+	380:383	arg1	effect					370:375	the effect	366:375	the effect of Ca2+ on the biosynthesis of EPS in the probiotic Lactobacillus plantarum K25	366:455	In this study, the effect of Ca2+ on the biosynthesis of EPS in the probiotic Lactobacillus plantarum K25 was studied.
33455763	1	101	theme	metal	253:257	arg1	calcium					235:241	calcium	235:241	calcium	235:241	Exopolysaccharide (EPS)-producing lactic acid bacteria have been widely used in dairy products, but how calcium, the main metal ion component in milk, regulates the EPS biosynthesis in lactic acid bacteria is not clear.
33455763	1	101	theme	metal	253:257	arg1	component					263:271	the main metal ion component	244:271	the main metal ion component in milk	244:279	Exopolysaccharide (EPS)-producing lactic acid bacteria have been widely used in dairy products, but how calcium, the main metal ion component in milk, regulates the EPS biosynthesis in lactic acid bacteria is not clear.
33455763	4	102	theme	spectral	924:931	arg1	analysis					933:940	spectral analysis	924:940	spectral analysis	924:940	The presence of Ca2+ also changed the monosaccharide composition of the EPS with decreased high molecular weight components and more content of rhamnose, though the functional groups of the polymer were not altered as revealed by Fourier transform infrared spectral analysis.
33455763	11	103	theme	related	2101:2107	arg1	pathways					2092:2099	metabolic pathways	2082:2099	metabolic pathways related to the EPS biosynthesis in L. plantarum K25 in response to Ca2+	2082:2171	This study provides a basis for understanding the overall change of metabolic pathways related to the EPS biosynthesis in L. plantarum K25 in response to Ca2+, facilitating exploitation of its EPS-producing potential for application in probiotic dairy products.
33455763	3	104	from	mg/L	518:521	arg1	addition					494:501	addition	494:501	addition of CaCl2 at 20 mg/L in a semi-defined medium	494:546	The results showed that addition of CaCl2 at 20 mg/L in a semi-defined medium did not affect the growth of strain K25, but it increased the EPS yield and changed the microstructure of the polymer.
33455763	3	104	from	mg/L	518:521	arg1	medium					541:546	a semi-defined medium	526:546	a semi-defined medium	526:546	The results showed that addition of CaCl2 at 20 mg/L in a semi-defined medium did not affect the growth of strain K25, but it increased the EPS yield and changed the microstructure of the polymer.
33455763	6	105	theme	ion	1289:1291	arg1	transport					1293:1301	ion transport	1289:1301	ion transport	1289:1301	Proteomics analysis showed that Ca2+ upregulated most of the proteins related to carbon transport and metabolism, fatty acid synthesis, amino acid synthesis, ion transport, UMP synthesis.
33455763	7	106	theme	PtlIID	1389:1394	arg1	expression					1344:1353	the increased expression	1330:1353	the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase	1330:1430	Specially, the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase provided substrates for the EPS synthesis.
33455763	9	107	theme	active	1676:1681	arg1	ribose					1744:1749	5-phosphate ribose	1732:1749	5-phosphate ribose	1732:1749	Among them the content of active small molecules such as polygalitol, lyxose, and 5-phosphate ribose increased, facilitating the EPS biosynthesis.
33455763	9	107	theme	active	1676:1681	arg1	lyxose					1720:1725	lyxose	1720:1725	lyxose	1720:1725	Among them the content of active small molecules such as polygalitol, lyxose, and 5-phosphate ribose increased, facilitating the EPS biosynthesis.
33455763	9	107	theme	active	1676:1681	arg1	molecules					1689:1697	active small molecules	1676:1697	active small molecules such as polygalitol, lyxose, and 5-phosphate ribose	1676:1749	Among them the content of active small molecules such as polygalitol, lyxose, and 5-phosphate ribose increased, facilitating the EPS biosynthesis.
33455763	9	107	theme	active	1676:1681	arg1	polygalitol					1707:1717	polygalitol	1707:1717	polygalitol	1707:1717	Among them the content of active small molecules such as polygalitol, lyxose, and 5-phosphate ribose increased, facilitating the EPS biosynthesis.
33455763	8	108	from	change	1529:1534	arg1	metabolism					1638:1647	propionic acid metabolism	1623:1647	propionic acid metabolism	1623:1647	Meanwhile, metabolomics analysis revealed significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism.
33455763	8	108	from	change	1529:1534	arg1	cycle					1593:1597	tricarboxylic acid cycle	1574:1597	tricarboxylic acid cycle	1574:1597	Meanwhile, metabolomics analysis revealed significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism.
33455763	8	108	from	change	1529:1534	arg1	metabolism					1608:1617	glucose metabolism	1600:1617	glucose metabolism	1600:1617	Meanwhile, metabolomics analysis revealed significant change of the small molecular metabolites in tricarboxylic acid cycle, glucose metabolism and propionic acid metabolism.
33455763	7	109	theme	EIIABC	1373:1378	arg1	expression					1344:1353	the increased expression	1330:1353	the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase	1330:1430	Specially, the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase provided substrates for the EPS synthesis.
33455763	9	110	theme	molecules	1689:1697	arg1	content					1665:1671	the content	1661:1671	the content of active small molecules such as polygalitol, lyxose, and 5-phosphate ribose	1661:1749	Among them the content of active small molecules such as polygalitol, lyxose, and 5-phosphate ribose increased, facilitating the EPS biosynthesis.
33455763	5	111	with	biosynthesis	1103:1114	arg1	rhamnose					1121:1128	rhamnose	1121:1128	rhamnose	1121:1128	These were further confirmed by analysis of the mRNA expression of cps genes, 9 of which were upregulated by Ca2+, including cps4F and rfbD associated with EPS biosynthesis with rhamnose.
33455763	5	112	theme	EPS	1099:1101	arg1	biosynthesis					1103:1114	EPS biosynthesis	1099:1114	EPS biosynthesis with rhamnose	1099:1128	These were further confirmed by analysis of the mRNA expression of cps genes, 9 of which were upregulated by Ca2+, including cps4F and rfbD associated with EPS biosynthesis with rhamnose.
33455763	7	113	theme	PtlIIBC	1364:1370	arg1	expression					1344:1353	the increased expression	1330:1353	the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase	1330:1430	Specially, the increased expression of MelB, PtlIIBC, EIIABC, PtlIIC, PtlIID, Bgl, GH1, MalFGK, DhaK, and FBPase provided substrates for the EPS synthesis.
34294304	5	0	theme	wound	1283:1287	arg1	potential					1260:1268	significant potential	1248:1268	significant potential for use	1248:1276	This study thus indicates that the composite Pκ-CaC hydrogel has significant potential for use as a wound dressing.
34294304	5	0	theme	wound	1283:1287	arg1	dressing					1289:1296	a wound dressing	1281:1296	a wound dressing	1281:1296	This study thus indicates that the composite Pκ-CaC hydrogel has significant potential for use as a wound dressing.
34294304	3	1	theme	three-dimensional	724:740	arg1	architecture					742:753	a uniformly thick, highly porous three-dimensional architecture	691:753	a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores	691:786	The results showed that the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel had a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores, a high fluid absorption, and retention capacity without disturbing its mechanical stability, and good in vitro biocompatibility.
34294304	4	2	theme	full-thickness	946:959	arg1	model					972:976	the full-thickness skin wound model	942:976	the full-thickness skin wound model	942:976	Macroscopic images from the full-thickness skin wound model revealed that the wounds dressed with the proposed Pκ-CaC hydrogel were completely healed by day 14, while the histomorphological results confirmed full re-epithelization and rapid skin-tissue remodeling.
34294304	1	3	theme	wound	179:183	arg1	dressing					185:192	an effective and mechanically robust wound dressing	142:192	an effective and mechanically robust wound dressing	142:192	To develop an effective and mechanically robust wound dressing, a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS) was prepared in a cassette via repeated freeze/thaw cycles, photo-crosslinking, and chemical cross-linking.
34294304	4	4	theme	Pκ-CaC	1029:1034	arg1	hydrogel					1036:1043	the proposed Pκ-CaC hydrogel	1016:1043	the proposed Pκ-CaC hydrogel	1016:1043	Macroscopic images from the full-thickness skin wound model revealed that the wounds dressed with the proposed Pκ-CaC hydrogel were completely healed by day 14, while the histomorphological results confirmed full re-epithelization and rapid skin-tissue remodeling.
34294304	1	5	theme	κ-CaMA	256:261	arg1	hydrogel					274:281	a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel	195:281	a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS)	195:328	To develop an effective and mechanically robust wound dressing, a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS) was prepared in a cassette via repeated freeze/thaw cycles, photo-crosslinking, and chemical cross-linking.
34294304	2	6	dep	in	474:475	arg1	vitro					477:481	vitro	477:481	vitro	477:481	The chemical, physical, mechanical, in vitro biocompatibility, in vivo wound-healing properties, and antibacterial activity of triple-crosslinked hydrogel were subsequently characterized.
34294304	3	7	with	absorption	802:811	arg1	pores					782:786	uniformly distributed pores	760:786	uniformly distributed pores	760:786	The results showed that the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel had a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores, a high fluid absorption, and retention capacity without disturbing its mechanical stability, and good in vitro biocompatibility.
34294304	5	8	theme	Pκ-CaC	1228:1233	arg1	hydrogel					1235:1242	the composite Pκ-CaC hydrogel	1214:1242	the composite Pκ-CaC hydrogel	1214:1242	This study thus indicates that the composite Pκ-CaC hydrogel has significant potential for use as a wound dressing.
34294304	0	9	theme	kappa-carrageenan/chitooligosaccharide	82:119	arg1	hydrogel					121:128	kappa-carrageenan/chitooligosaccharide hydrogel	82:128	kappa-carrageenan/chitooligosaccharide hydrogel	82:128	Wound healing properties of triple cross-linked poly (vinyl alcohol)/methacrylate kappa-carrageenan/chitooligosaccharide hydrogel.
34294304	4	10	theme	Macroscopic	918:928	arg1	images					930:935	Macroscopic images	918:935	Macroscopic images from the full-thickness skin wound model	918:976	Macroscopic images from the full-thickness skin wound model revealed that the wounds dressed with the proposed Pκ-CaC hydrogel were completely healed by day 14, while the histomorphological results confirmed full re-epithelization and rapid skin-tissue remodeling.
34294304	3	11	dep	in	891:892	arg1	vitro					894:898	vitro	894:898	vitro	894:898	The results showed that the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel had a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores, a high fluid absorption, and retention capacity without disturbing its mechanical stability, and good in vitro biocompatibility.
34294304	4	12	theme	proposed	1020:1027	arg1	hydrogel					1036:1043	the proposed Pκ-CaC hydrogel	1016:1043	the proposed Pκ-CaC hydrogel	1016:1043	Macroscopic images from the full-thickness skin wound model revealed that the wounds dressed with the proposed Pκ-CaC hydrogel were completely healed by day 14, while the histomorphological results confirmed full re-epithelization and rapid skin-tissue remodeling.
34294304	1	13	theme	composite	264:272	arg1	hydrogel					274:281	a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel	195:281	a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS)	195:328	To develop an effective and mechanically robust wound dressing, a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS) was prepared in a cassette via repeated freeze/thaw cycles, photo-crosslinking, and chemical cross-linking.
34294304	2	14	theme	wound-healing	509:521	arg1	properties					523:532	in vivo wound-healing properties	501:532	in vivo wound-healing properties	501:532	The chemical, physical, mechanical, in vitro biocompatibility, in vivo wound-healing properties, and antibacterial activity of triple-crosslinked hydrogel were subsequently characterized.
34294304	3	15	theme	distributed	770:780	arg1	pores					782:786	uniformly distributed pores	760:786	uniformly distributed pores	760:786	The results showed that the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel had a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores, a high fluid absorption, and retention capacity without disturbing its mechanical stability, and good in vitro biocompatibility.
34294304	2	16	dep	chemical	442:449	arg1	mechanical					462:471	mechanical	462:471	mechanical	462:471	The chemical, physical, mechanical, in vitro biocompatibility, in vivo wound-healing properties, and antibacterial activity of triple-crosslinked hydrogel were subsequently characterized.
34294304	2	16	dep	chemical	442:449	arg1	physical					452:459	physical	452:459	physical	452:459	The chemical, physical, mechanical, in vitro biocompatibility, in vivo wound-healing properties, and antibacterial activity of triple-crosslinked hydrogel were subsequently characterized.
34294304	2	16	dep	chemical	442:449	arg1	in					474:475	in	474:475	in	474:475	The chemical, physical, mechanical, in vitro biocompatibility, in vivo wound-healing properties, and antibacterial activity of triple-crosslinked hydrogel were subsequently characterized.
34294304	3	17	dep	thick	703:707	arg1	porous					717:722	porous	717:722	porous	717:722	The results showed that the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel had a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores, a high fluid absorption, and retention capacity without disturbing its mechanical stability, and good in vitro biocompatibility.
34294304	3	18	theme	mechanical	860:869	arg1	stability					871:879	mechanical stability	860:879	mechanical stability	860:879	The results showed that the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel had a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores, a high fluid absorption, and retention capacity without disturbing its mechanical stability, and good in vitro biocompatibility.
34294304	1	19	theme	repeated	361:368	arg1	cycles					382:387	repeated freeze/thaw cycles	361:387	repeated freeze/thaw cycles	361:387	To develop an effective and mechanically robust wound dressing, a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS) was prepared in a cassette via repeated freeze/thaw cycles, photo-crosslinking, and chemical cross-linking.
34294304	3	20	contain	had	687:689	arg2	absorption					802:811	a high fluid absorption	789:811	a high fluid absorption	789:811	The results showed that the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel had a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores, a high fluid absorption, and retention capacity without disturbing its mechanical stability, and good in vitro biocompatibility.
34294304	3	20	contain	had	687:689	arg1	hydrogel					678:685	the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel	650:685	the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel	650:685	The results showed that the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel had a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores, a high fluid absorption, and retention capacity without disturbing its mechanical stability, and good in vitro biocompatibility.
34294304	3	20	contain	had	687:689	arg2	architecture					742:753	a uniformly thick, highly porous three-dimensional architecture	691:753	a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores	691:786	The results showed that the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel had a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores, a high fluid absorption, and retention capacity without disturbing its mechanical stability, and good in vitro biocompatibility.
34294304	3	20	contain	had	687:689	arg2	capacity					828:835	retention capacity	818:835	retention capacity	818:835	The results showed that the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel had a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores, a high fluid absorption, and retention capacity without disturbing its mechanical stability, and good in vitro biocompatibility.
34294304	0	21	theme	healing	6:12	arg1	properties					14:23	Wound healing properties	0:23	Wound healing properties of triple cross-linked poly (vinyl alcohol)	0:67	Wound healing properties of triple cross-linked poly (vinyl alcohol)/methacrylate kappa-carrageenan/chitooligosaccharide hydrogel.
34294304	1	22	theme	freeze/thaw	370:380	arg1	cycles					382:387	repeated freeze/thaw cycles	361:387	repeated freeze/thaw cycles	361:387	To develop an effective and mechanically robust wound dressing, a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS) was prepared in a cassette via repeated freeze/thaw cycles, photo-crosslinking, and chemical cross-linking.
34294304	5	23	theme	significant	1248:1258	arg1	potential					1260:1268	significant potential	1248:1268	significant potential for use	1248:1276	This study thus indicates that the composite Pκ-CaC hydrogel has significant potential for use as a wound dressing.
34294304	5	23	theme	significant	1248:1258	arg1	dressing					1289:1296	a wound dressing	1281:1296	a wound dressing	1281:1296	This study thus indicates that the composite Pκ-CaC hydrogel has significant potential for use as a wound dressing.
34294304	2	24	theme	in	501:502	arg1	properties					523:532	in vivo wound-healing properties	501:532	in vivo wound-healing properties	501:532	The chemical, physical, mechanical, in vitro biocompatibility, in vivo wound-healing properties, and antibacterial activity of triple-crosslinked hydrogel were subsequently characterized.
34294304	1	25	theme	poly	197:200	arg1	hydrogel					274:281	a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel	195:281	a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS)	195:328	To develop an effective and mechanically robust wound dressing, a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS) was prepared in a cassette via repeated freeze/thaw cycles, photo-crosslinking, and chemical cross-linking.
34294304	0	26	theme	Wound	0:4	arg1	healing					6:12	Wound healing	0:12	Wound healing properties of triple cross-linked poly (vinyl alcohol)	0:67	Wound healing properties of triple cross-linked poly (vinyl alcohol)/methacrylate kappa-carrageenan/chitooligosaccharide hydrogel.
34294304	0	27	link	cross-linked	35:46	arg1	alcohol					60:66	triple cross-linked poly (vinyl alcohol)	28:67	triple cross-linked poly (vinyl alcohol)	28:67	Wound healing properties of triple cross-linked poly (vinyl alcohol)/methacrylate kappa-carrageenan/chitooligosaccharide hydrogel.
34294304	2	28	theme	chemical	442:449	arg1	biocompatibility					483:498	The chemical, physical, mechanical, in vitro biocompatibility	438:498	The chemical, physical, mechanical, in vitro biocompatibility	438:498	The chemical, physical, mechanical, in vitro biocompatibility, in vivo wound-healing properties, and antibacterial activity of triple-crosslinked hydrogel were subsequently characterized.
34294304	3	29	theme	high	791:794	arg1	absorption					802:811	a high fluid absorption	789:811	a high fluid absorption	789:811	The results showed that the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel had a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores, a high fluid absorption, and retention capacity without disturbing its mechanical stability, and good in vitro biocompatibility.
34294304	2	30	link	triple-crosslinked	565:582	arg1	hydrogel					584:591	triple-crosslinked hydrogel	565:591	triple-crosslinked hydrogel	565:591	The chemical, physical, mechanical, in vitro biocompatibility, in vivo wound-healing properties, and antibacterial activity of triple-crosslinked hydrogel were subsequently characterized.
34294304	1	31	theme	vinyl	203:207	arg1	hydrogel					274:281	a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel	195:281	a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS)	195:328	To develop an effective and mechanically robust wound dressing, a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS) was prepared in a cassette via repeated freeze/thaw cycles, photo-crosslinking, and chemical cross-linking.
34294304	4	32	theme	wound	966:970	arg1	model					972:976	the full-thickness skin wound model	942:976	the full-thickness skin wound model	942:976	Macroscopic images from the full-thickness skin wound model revealed that the wounds dressed with the proposed Pκ-CaC hydrogel were completely healed by day 14, while the histomorphological results confirmed full re-epithelization and rapid skin-tissue remodeling.
34294304	1	33	theme	alcohol	209:215	arg1	hydrogel					274:281	a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel	195:281	a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS)	195:328	To develop an effective and mechanically robust wound dressing, a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS) was prepared in a cassette via repeated freeze/thaw cycles, photo-crosslinking, and chemical cross-linking.
34294304	0	34	theme	cross-linked	35:46	arg1	alcohol					60:66	triple cross-linked poly (vinyl alcohol)	28:67	triple cross-linked poly (vinyl alcohol)	28:67	Wound healing properties of triple cross-linked poly (vinyl alcohol)/methacrylate kappa-carrageenan/chitooligosaccharide hydrogel.
34294304	2	35	theme	antibacterial	539:551	arg1	activity					553:560	antibacterial activity	539:560	antibacterial activity of triple-crosslinked hydrogel	539:591	The chemical, physical, mechanical, in vitro biocompatibility, in vivo wound-healing properties, and antibacterial activity of triple-crosslinked hydrogel were subsequently characterized.
34294304	0	36	theme	triple	28:33	arg1	alcohol					60:66	triple cross-linked poly (vinyl alcohol)	28:67	triple cross-linked poly (vinyl alcohol)	28:67	Wound healing properties of triple cross-linked poly (vinyl alcohol)/methacrylate kappa-carrageenan/chitooligosaccharide hydrogel.
34294304	5	37	contain	has	1244:1246	arg2	potential					1260:1268	significant potential	1248:1268	significant potential for use	1248:1276	This study thus indicates that the composite Pκ-CaC hydrogel has significant potential for use as a wound dressing.
34294304	5	37	contain	has	1244:1246	arg1	hydrogel					1235:1242	the composite Pκ-CaC hydrogel	1214:1242	the composite Pκ-CaC hydrogel	1214:1242	This study thus indicates that the composite Pκ-CaC hydrogel has significant potential for use as a wound dressing.
34294304	5	37	contain	has	1244:1246	arg2	dressing					1289:1296	a wound dressing	1281:1296	a wound dressing	1281:1296	This study thus indicates that the composite Pκ-CaC hydrogel has significant potential for use as a wound dressing.
34294304	3	38	with	architecture	742:753	arg1	pores					782:786	uniformly distributed pores	760:786	uniformly distributed pores	760:786	The results showed that the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel had a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores, a high fluid absorption, and retention capacity without disturbing its mechanical stability, and good in vitro biocompatibility.
34294304	3	39	theme	retention	818:826	arg1	capacity					828:835	retention capacity	818:835	retention capacity	818:835	The results showed that the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel had a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores, a high fluid absorption, and retention capacity without disturbing its mechanical stability, and good in vitro biocompatibility.
34294304	5	40	theme	composite	1218:1226	arg1	hydrogel					1235:1242	the composite Pκ-CaC hydrogel	1214:1242	the composite Pκ-CaC hydrogel	1214:1242	This study thus indicates that the composite Pκ-CaC hydrogel has significant potential for use as a wound dressing.
34294304	3	41	theme	Pκ-CaC	670:675	arg1	hydrogel					678:685	the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel	650:685	the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel	650:685	The results showed that the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel had a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores, a high fluid absorption, and retention capacity without disturbing its mechanical stability, and good in vitro biocompatibility.
34294304	2	42	dep	in	501:502	arg1	vivo					504:507	vivo	504:507	vivo	504:507	The chemical, physical, mechanical, in vitro biocompatibility, in vivo wound-healing properties, and antibacterial activity of triple-crosslinked hydrogel were subsequently characterized.
34294304	0	43	theme	poly	48:51	arg1	alcohol					60:66	triple cross-linked poly (vinyl alcohol)	28:67	triple cross-linked poly (vinyl alcohol)	28:67	Wound healing properties of triple cross-linked poly (vinyl alcohol)/methacrylate kappa-carrageenan/chitooligosaccharide hydrogel.
34294304	3	44	theme	fluid	796:800	arg1	absorption					802:811	a high fluid absorption	789:811	a high fluid absorption	789:811	The results showed that the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel had a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores, a high fluid absorption, and retention capacity without disturbing its mechanical stability, and good in vitro biocompatibility.
34294304	3	45	theme	thick	703:707	arg1	architecture					742:753	a uniformly thick, highly porous three-dimensional architecture	691:753	a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores	691:786	The results showed that the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel had a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores, a high fluid absorption, and retention capacity without disturbing its mechanical stability, and good in vitro biocompatibility.
34294304	4	46	from	model	972:976	arg1	images					930:935	Macroscopic images	918:935	Macroscopic images from the full-thickness skin wound model	918:976	Macroscopic images from the full-thickness skin wound model revealed that the wounds dressed with the proposed Pκ-CaC hydrogel were completely healed by day 14, while the histomorphological results confirmed full re-epithelization and rapid skin-tissue remodeling.
34294304	3	47	theme	in	891:892	arg1	biocompatibility					900:915	good in vitro biocompatibility	886:915	good in vitro biocompatibility	886:915	The results showed that the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel had a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores, a high fluid absorption, and retention capacity without disturbing its mechanical stability, and good in vitro biocompatibility.
34294304	2	48	theme	hydrogel	584:591	arg1	activity					553:560	antibacterial activity	539:560	antibacterial activity of triple-crosslinked hydrogel	539:591	The chemical, physical, mechanical, in vitro biocompatibility, in vivo wound-healing properties, and antibacterial activity of triple-crosslinked hydrogel were subsequently characterized.
34294304	2	48	theme	hydrogel	584:591	arg1	properties					523:532	in vivo wound-healing properties	501:532	in vivo wound-healing properties	501:532	The chemical, physical, mechanical, in vitro biocompatibility, in vivo wound-healing properties, and antibacterial activity of triple-crosslinked hydrogel were subsequently characterized.
34294304	2	48	theme	hydrogel	584:591	arg1	biocompatibility					483:498	The chemical, physical, mechanical, in vitro biocompatibility	438:498	The chemical, physical, mechanical, in vitro biocompatibility	438:498	The chemical, physical, mechanical, in vitro biocompatibility, in vivo wound-healing properties, and antibacterial activity of triple-crosslinked hydrogel were subsequently characterized.
34294304	1	49	theme	chemical	414:421	arg1	cross-linking					423:435	chemical cross-linking	414:435	chemical cross-linking	414:435	To develop an effective and mechanically robust wound dressing, a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS) was prepared in a cassette via repeated freeze/thaw cycles, photo-crosslinking, and chemical cross-linking.
34294304	1	50	theme	effective	145:153	arg1	dressing					185:192	an effective and mechanically robust wound dressing	142:192	an effective and mechanically robust wound dressing	142:192	To develop an effective and mechanically robust wound dressing, a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS) was prepared in a cassette via repeated freeze/thaw cycles, photo-crosslinking, and chemical cross-linking.
34294304	4	51	theme	full	1126:1129	arg1	re-epithelization					1131:1147	full re-epithelization	1126:1147	full re-epithelization	1126:1147	Macroscopic images from the full-thickness skin wound model revealed that the wounds dressed with the proposed Pκ-CaC hydrogel were completely healed by day 14, while the histomorphological results confirmed full re-epithelization and rapid skin-tissue remodeling.
34294304	1	52	theme	PVA	219:221	arg1	hydrogel					274:281	a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel	195:281	a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS)	195:328	To develop an effective and mechanically robust wound dressing, a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS) was prepared in a cassette via repeated freeze/thaw cycles, photo-crosslinking, and chemical cross-linking.
34294304	2	53	theme	triple-crosslinked	565:582	arg1	hydrogel					584:591	triple-crosslinked hydrogel	565:591	triple-crosslinked hydrogel	565:591	The chemical, physical, mechanical, in vitro biocompatibility, in vivo wound-healing properties, and antibacterial activity of triple-crosslinked hydrogel were subsequently characterized.
34294304	3	54	theme	PVA/κ-CaMA/COS	654:667	arg1	hydrogel					678:685	the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel	650:685	the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel	650:685	The results showed that the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel had a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores, a high fluid absorption, and retention capacity without disturbing its mechanical stability, and good in vitro biocompatibility.
34294304	4	55	theme	skin	961:964	arg1	model					972:976	the full-thickness skin wound model	942:976	the full-thickness skin wound model	942:976	Macroscopic images from the full-thickness skin wound model revealed that the wounds dressed with the proposed Pκ-CaC hydrogel were completely healed by day 14, while the histomorphological results confirmed full re-epithelization and rapid skin-tissue remodeling.
34294304	4	56	theme	skin-tissue	1159:1169	arg1	remodeling					1171:1180	rapid skin-tissue remodeling	1153:1180	rapid skin-tissue remodeling	1153:1180	Macroscopic images from the full-thickness skin wound model revealed that the wounds dressed with the proposed Pκ-CaC hydrogel were completely healed by day 14, while the histomorphological results confirmed full re-epithelization and rapid skin-tissue remodeling.
34294304	4	57	theme	rapid	1153:1157	arg1	remodeling					1171:1180	rapid skin-tissue remodeling	1153:1180	rapid skin-tissue remodeling	1153:1180	Macroscopic images from the full-thickness skin wound model revealed that the wounds dressed with the proposed Pκ-CaC hydrogel were completely healed by day 14, while the histomorphological results confirmed full re-epithelization and rapid skin-tissue remodeling.
34294304	0	58	theme	alcohol	60:66	arg1	properties					14:23	Wound healing properties	0:23	Wound healing properties of triple cross-linked poly (vinyl alcohol)	0:67	Wound healing properties of triple cross-linked poly (vinyl alcohol)/methacrylate kappa-carrageenan/chitooligosaccharide hydrogel.
34294304	3	59	with	capacity	828:835	arg1	pores					782:786	uniformly distributed pores	760:786	uniformly distributed pores	760:786	The results showed that the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel had a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores, a high fluid absorption, and retention capacity without disturbing its mechanical stability, and good in vitro biocompatibility.
34294304	3	60	theme	good	886:889	arg1	biocompatibility					900:915	good in vitro biocompatibility	886:915	good in vitro biocompatibility	886:915	The results showed that the PVA/κ-CaMA/COS (Pκ-CaC) hydrogel had a uniformly thick, highly porous three-dimensional architecture with uniformly distributed pores, a high fluid absorption, and retention capacity without disturbing its mechanical stability, and good in vitro biocompatibility.
34294304	1	61	theme	/methacrylate	223:235	arg1	hydrogel					274:281	a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel	195:281	a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS)	195:328	To develop an effective and mechanically robust wound dressing, a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS) was prepared in a cassette via repeated freeze/thaw cycles, photo-crosslinking, and chemical cross-linking.
34294304	0	62	theme	vinyl	54:58	arg1	alcohol					60:66	triple cross-linked poly (vinyl alcohol)	28:67	triple cross-linked poly (vinyl alcohol)	28:67	Wound healing properties of triple cross-linked poly (vinyl alcohol)/methacrylate kappa-carrageenan/chitooligosaccharide hydrogel.
34294304	1	63	theme	robust	172:177	arg1	dressing					185:192	an effective and mechanically robust wound dressing	142:192	an effective and mechanically robust wound dressing	142:192	To develop an effective and mechanically robust wound dressing, a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS) was prepared in a cassette via repeated freeze/thaw cycles, photo-crosslinking, and chemical cross-linking.
34294304	4	64	theme	histomorphological	1089:1106	arg1	results					1108:1114	the histomorphological results	1085:1114	the histomorphological results	1085:1114	Macroscopic images from the full-thickness skin wound model revealed that the wounds dressed with the proposed Pκ-CaC hydrogel were completely healed by day 14, while the histomorphological results confirmed full re-epithelization and rapid skin-tissue remodeling.
34294304	1	65	theme	kappa-carrageenan	237:253	arg1	hydrogel					274:281	a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel	195:281	a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS)	195:328	To develop an effective and mechanically robust wound dressing, a poly (vinyl alcohol) (PVA)/methacrylate kappa-carrageenan (κ-CaMA) composite hydrogel encapsulated with a chitooligosaccharide (COS) was prepared in a cassette via repeated freeze/thaw cycles, photo-crosslinking, and chemical cross-linking.
32919574	3	0	theme	NaBH4	669:673	arg1	presence					657:664	the presence	653:664	the presence of NaBH4	653:673	The AgNPs were synthesized in situ in the PVA-GG hydrogel from various concentrations of the AgNO3 precursor solution in the presence of NaBH4.
32919574	6	1	theme	PVA-GG-AgNPs	995:1006	arg1	composite					1017:1025	The PVA-GG-AgNPs hydrogel composite	991:1025	The PVA-GG-AgNPs hydrogel composite	991:1025	The PVA-GG-AgNPs hydrogel composite exhibited excellent catalytic activity and antibacterial property, which makes them a suitable candidate for industrial applications.
32919574	2	2	theme	rapid	346:350	arg1	synthesis					366:374	a rapid and efficient synthesis	344:374	a rapid and efficient synthesis of AgNPs containing polyvinyl alcohol (PVA)-guar gum (GG) smart hydrogel composite, which exhibited pH-dependent swelling and enhanced mechanical strength	344:529	We report a rapid and efficient synthesis of AgNPs containing polyvinyl alcohol (PVA)-guar gum (GG) smart hydrogel composite, which exhibited pH-dependent swelling and enhanced mechanical strength.
32919574	2	3	theme	mechanical	511:520	arg1	strength					522:529	enhanced mechanical strength	502:529	enhanced mechanical strength	502:529	We report a rapid and efficient synthesis of AgNPs containing polyvinyl alcohol (PVA)-guar gum (GG) smart hydrogel composite, which exhibited pH-dependent swelling and enhanced mechanical strength.
32919574	5	4	theme	tensile	832:838	arg1	strength					840:847	tensile strength	832:847	tensile strength	832:847	Simultaneously, at the optimum concentration of AgNO3 (0.01 M), the tensile strength and elongation at break were enhanced by 74 % and 11 %, and swelling capacity was increased by 18 % as compared to PVA-GG hydrogel (control).
32919574	0	5	theme	catalytic	90:98	arg1	applications					115:126	catalytic and biomedical applications	90:126	catalytic and biomedical applications	90:126	Highly stable silver nanoparticles containing guar gum modified dual network hydrogel for catalytic and biomedical applications.
32919574	0	6	contain	containing	35:44	arg2	gum					51:53	guar gum	46:53	guar gum	46:53	Highly stable silver nanoparticles containing guar gum modified dual network hydrogel for catalytic and biomedical applications.
32919574	0	6	contain	containing	35:44	arg1	nanoparticles					21:33	Highly stable silver nanoparticles	0:33	Highly stable silver nanoparticles containing guar gum	0:53	Highly stable silver nanoparticles containing guar gum modified dual network hydrogel for catalytic and biomedical applications.
32919574	1	7	contain	possess	283:289	arg2	applicability					319:331	enhanced applicability	310:331	enhanced applicability	310:331	Although many preparation methods have been reported till date, it is still a great challenge to prepare silver nanoparticles (AgNPs) that simultaneously possess high stability and enhanced applicability.
32919574	1	7	contain	possess	283:289	arg1	AgNPs					256:260	AgNPs	256:260	AgNPs	256:260	Although many preparation methods have been reported till date, it is still a great challenge to prepare silver nanoparticles (AgNPs) that simultaneously possess high stability and enhanced applicability.
32919574	1	7	contain	possess	283:289	arg2	stability					296:304	high stability	291:304	high stability	291:304	Although many preparation methods have been reported till date, it is still a great challenge to prepare silver nanoparticles (AgNPs) that simultaneously possess high stability and enhanced applicability.
32919574	1	7	contain	possess	283:289	arg1	nanoparticles					241:253	silver nanoparticles	234:253	silver nanoparticles (AgNPs) that simultaneously possess high stability and enhanced applicability	234:331	Although many preparation methods have been reported till date, it is still a great challenge to prepare silver nanoparticles (AgNPs) that simultaneously possess high stability and enhanced applicability.
32919574	4	8	theme	Stable	676:681	arg1	AgNPs					683:687	Stable AgNPs	676:687	Stable AgNPs (90 days) of 10-20 nm uniformly dispersed in PVA-GG hydrogel	676:748	Stable AgNPs (90 days) of 10-20 nm uniformly dispersed in PVA-GG hydrogel was obtained.
32919574	4	8	theme	Stable	676:681	arg1	days					693:696	90 days	690:696	90 days	690:696	Stable AgNPs (90 days) of 10-20 nm uniformly dispersed in PVA-GG hydrogel was obtained.
32919574	1	9	theme	till	182:185	arg1	date					187:190	till date	182:190	till date	182:190	Although many preparation methods have been reported till date, it is still a great challenge to prepare silver nanoparticles (AgNPs) that simultaneously possess high stability and enhanced applicability.
32919574	0	10	theme	biomedical	104:113	arg1	applications					115:126	catalytic and biomedical applications	90:126	catalytic and biomedical applications	90:126	Highly stable silver nanoparticles containing guar gum modified dual network hydrogel for catalytic and biomedical applications.
32919574	5	11	from	break	867:871	arg1	strength					840:847	tensile strength	832:847	tensile strength	832:847	Simultaneously, at the optimum concentration of AgNO3 (0.01 M), the tensile strength and elongation at break were enhanced by 74 % and 11 %, and swelling capacity was increased by 18 % as compared to PVA-GG hydrogel (control).
32919574	5	11	from	break	867:871	arg1	elongation					853:862	elongation	853:862	elongation	853:862	Simultaneously, at the optimum concentration of AgNO3 (0.01 M), the tensile strength and elongation at break were enhanced by 74 % and 11 %, and swelling capacity was increased by 18 % as compared to PVA-GG hydrogel (control).
32919574	4	12	theme	PVA-GG	734:739	arg1	hydrogel					741:748	PVA-GG hydrogel	734:748	PVA-GG hydrogel	734:748	Stable AgNPs (90 days) of 10-20 nm uniformly dispersed in PVA-GG hydrogel was obtained.
32919574	0	13	theme	stable	7:12	arg1	nanoparticles					21:33	Highly stable silver nanoparticles	0:33	Highly stable silver nanoparticles containing guar gum	0:53	Highly stable silver nanoparticles containing guar gum modified dual network hydrogel for catalytic and biomedical applications.
32919574	2	14	theme	pH-dependent	476:487	arg1	swelling					489:496	pH-dependent swelling	476:496	pH-dependent swelling	476:496	We report a rapid and efficient synthesis of AgNPs containing polyvinyl alcohol (PVA)-guar gum (GG) smart hydrogel composite, which exhibited pH-dependent swelling and enhanced mechanical strength.
32919574	5	15	dep	strength	840:847	arg1	the					828:830	the	828:830	the	828:830	Simultaneously, at the optimum concentration of AgNO3 (0.01 M), the tensile strength and elongation at break were enhanced by 74 % and 11 %, and swelling capacity was increased by 18 % as compared to PVA-GG hydrogel (control).
32919574	2	16	theme	AgNPs	379:383	arg1	synthesis					366:374	a rapid and efficient synthesis	344:374	a rapid and efficient synthesis of AgNPs containing polyvinyl alcohol (PVA)-guar gum (GG) smart hydrogel composite, which exhibited pH-dependent swelling and enhanced mechanical strength	344:529	We report a rapid and efficient synthesis of AgNPs containing polyvinyl alcohol (PVA)-guar gum (GG) smart hydrogel composite, which exhibited pH-dependent swelling and enhanced mechanical strength.
32919574	6	17	theme	antibacterial	1070:1082	arg1	property					1084:1091	antibacterial property	1070:1091	antibacterial property	1070:1091	The PVA-GG-AgNPs hydrogel composite exhibited excellent catalytic activity and antibacterial property, which makes them a suitable candidate for industrial applications.
32919574	2	18	theme	gum	425:427	arg1	composite					449:457	-guar gum (GG) smart hydrogel composite	419:457	-guar gum (GG) smart hydrogel composite	419:457	We report a rapid and efficient synthesis of AgNPs containing polyvinyl alcohol (PVA)-guar gum (GG) smart hydrogel composite, which exhibited pH-dependent swelling and enhanced mechanical strength.
32919574	0	19	theme	silver	14:19	arg1	nanoparticles					21:33	Highly stable silver nanoparticles	0:33	Highly stable silver nanoparticles containing guar gum	0:53	Highly stable silver nanoparticles containing guar gum modified dual network hydrogel for catalytic and biomedical applications.
32919574	3	20	theme	precursor	631:639	arg1	solution					641:648	the AgNO3 precursor solution	621:648	the AgNO3 precursor solution	621:648	The AgNPs were synthesized in situ in the PVA-GG hydrogel from various concentrations of the AgNO3 precursor solution in the presence of NaBH4.
32919574	4	21	theme	nm	708:709	arg1	AgNPs					683:687	Stable AgNPs	676:687	Stable AgNPs (90 days) of 10-20 nm uniformly dispersed in PVA-GG hydrogel	676:748	Stable AgNPs (90 days) of 10-20 nm uniformly dispersed in PVA-GG hydrogel was obtained.
32919574	4	21	theme	nm	708:709	arg1	days					693:696	90 days	690:696	90 days	690:696	Stable AgNPs (90 days) of 10-20 nm uniformly dispersed in PVA-GG hydrogel was obtained.
32919574	0	22	theme	guar	46:49	arg1	gum					51:53	guar gum	46:53	guar gum	46:53	Highly stable silver nanoparticles containing guar gum modified dual network hydrogel for catalytic and biomedical applications.
32919574	1	23	theme	high	291:294	arg1	stability					296:304	high stability	291:304	high stability	291:304	Although many preparation methods have been reported till date, it is still a great challenge to prepare silver nanoparticles (AgNPs) that simultaneously possess high stability and enhanced applicability.
32919574	3	24	theme	solution	641:648	arg1	concentrations					603:616	various concentrations	595:616	various concentrations of the AgNO3 precursor solution	595:648	The AgNPs were synthesized in situ in the PVA-GG hydrogel from various concentrations of the AgNO3 precursor solution in the presence of NaBH4.
32919574	3	25	theme	PVA-GG	574:579	arg1	hydrogel					581:588	the PVA-GG hydrogel	570:588	the PVA-GG hydrogel	570:588	The AgNPs were synthesized in situ in the PVA-GG hydrogel from various concentrations of the AgNO3 precursor solution in the presence of NaBH4.
32919574	5	26	theme	AgNO3	812:816	arg1	M					824:824	0.01 M	819:824	0.01 M	819:824	Simultaneously, at the optimum concentration of AgNO3 (0.01 M), the tensile strength and elongation at break were enhanced by 74 % and 11 %, and swelling capacity was increased by 18 % as compared to PVA-GG hydrogel (control).
32919574	5	26	theme	AgNO3	812:816	arg1	concentration					795:807	the optimum concentration	783:807	the optimum concentration of AgNO3 (0.01 M)	783:825	Simultaneously, at the optimum concentration of AgNO3 (0.01 M), the tensile strength and elongation at break were enhanced by 74 % and 11 %, and swelling capacity was increased by 18 % as compared to PVA-GG hydrogel (control).
32919574	2	27	theme	polyvinyl	396:404	arg1	PVA					415:417	PVA	415:417	PVA	415:417	We report a rapid and efficient synthesis of AgNPs containing polyvinyl alcohol (PVA)-guar gum (GG) smart hydrogel composite, which exhibited pH-dependent swelling and enhanced mechanical strength.
32919574	2	27	theme	polyvinyl	396:404	arg1	alcohol					406:412	polyvinyl alcohol	396:412	polyvinyl alcohol (PVA)	396:418	We report a rapid and efficient synthesis of AgNPs containing polyvinyl alcohol (PVA)-guar gum (GG) smart hydrogel composite, which exhibited pH-dependent swelling and enhanced mechanical strength.
32919574	2	28	theme	hydrogel	440:447	arg1	composite					449:457	-guar gum (GG) smart hydrogel composite	419:457	-guar gum (GG) smart hydrogel composite	419:457	We report a rapid and efficient synthesis of AgNPs containing polyvinyl alcohol (PVA)-guar gum (GG) smart hydrogel composite, which exhibited pH-dependent swelling and enhanced mechanical strength.
32919574	2	29	contain	containing	385:394	arg2	PVA					415:417	PVA	415:417	PVA	415:417	We report a rapid and efficient synthesis of AgNPs containing polyvinyl alcohol (PVA)-guar gum (GG) smart hydrogel composite, which exhibited pH-dependent swelling and enhanced mechanical strength.
32919574	2	29	contain	containing	385:394	arg1	AgNPs					379:383	AgNPs	379:383	AgNPs containing polyvinyl alcohol (PVA)	379:418	We report a rapid and efficient synthesis of AgNPs containing polyvinyl alcohol (PVA)-guar gum (GG) smart hydrogel composite, which exhibited pH-dependent swelling and enhanced mechanical strength.
32919574	2	29	contain	containing	385:394	arg2	alcohol					406:412	polyvinyl alcohol	396:412	polyvinyl alcohol (PVA)	396:418	We report a rapid and efficient synthesis of AgNPs containing polyvinyl alcohol (PVA)-guar gum (GG) smart hydrogel composite, which exhibited pH-dependent swelling and enhanced mechanical strength.
32919574	1	30	theme	great	207:211	arg1	challenge					213:221	a great challenge	205:221	a great challenge to prepare silver nanoparticles (AgNPs) that simultaneously possess high stability and enhanced applicability	205:331	Although many preparation methods have been reported till date, it is still a great challenge to prepare silver nanoparticles (AgNPs) that simultaneously possess high stability and enhanced applicability.
32919574	1	30	theme	great	207:211	arg1	it					193:194	it	193:194	it	193:194	Although many preparation methods have been reported till date, it is still a great challenge to prepare silver nanoparticles (AgNPs) that simultaneously possess high stability and enhanced applicability.
32919574	2	31	theme	smart	434:438	arg1	composite					449:457	-guar gum (GG) smart hydrogel composite	419:457	-guar gum (GG) smart hydrogel composite	419:457	We report a rapid and efficient synthesis of AgNPs containing polyvinyl alcohol (PVA)-guar gum (GG) smart hydrogel composite, which exhibited pH-dependent swelling and enhanced mechanical strength.
32919574	5	32	theme	optimum	787:793	arg1	M					824:824	0.01 M	819:824	0.01 M	819:824	Simultaneously, at the optimum concentration of AgNO3 (0.01 M), the tensile strength and elongation at break were enhanced by 74 % and 11 %, and swelling capacity was increased by 18 % as compared to PVA-GG hydrogel (control).
32919574	5	32	theme	optimum	787:793	arg1	concentration					795:807	the optimum concentration	783:807	the optimum concentration of AgNO3 (0.01 M)	783:825	Simultaneously, at the optimum concentration of AgNO3 (0.01 M), the tensile strength and elongation at break were enhanced by 74 % and 11 %, and swelling capacity was increased by 18 % as compared to PVA-GG hydrogel (control).
32919574	3	33	theme	AgNO3	625:629	arg1	solution					641:648	the AgNO3 precursor solution	621:648	the AgNO3 precursor solution	621:648	The AgNPs were synthesized in situ in the PVA-GG hydrogel from various concentrations of the AgNO3 precursor solution in the presence of NaBH4.
32919574	2	34	theme	enhanced	502:509	arg1	strength					522:529	enhanced mechanical strength	502:529	enhanced mechanical strength	502:529	We report a rapid and efficient synthesis of AgNPs containing polyvinyl alcohol (PVA)-guar gum (GG) smart hydrogel composite, which exhibited pH-dependent swelling and enhanced mechanical strength.
32919574	6	35	theme	catalytic	1047:1055	arg1	activity					1057:1064	excellent catalytic activity	1037:1064	excellent catalytic activity	1037:1064	The PVA-GG-AgNPs hydrogel composite exhibited excellent catalytic activity and antibacterial property, which makes them a suitable candidate for industrial applications.
32919574	2	36	theme	efficient	356:364	arg1	synthesis					366:374	a rapid and efficient synthesis	344:374	a rapid and efficient synthesis of AgNPs containing polyvinyl alcohol (PVA)-guar gum (GG) smart hydrogel composite, which exhibited pH-dependent swelling and enhanced mechanical strength	344:529	We report a rapid and efficient synthesis of AgNPs containing polyvinyl alcohol (PVA)-guar gum (GG) smart hydrogel composite, which exhibited pH-dependent swelling and enhanced mechanical strength.
32919574	1	37	theme	enhanced	310:317	arg1	applicability					319:331	enhanced applicability	310:331	enhanced applicability	310:331	Although many preparation methods have been reported till date, it is still a great challenge to prepare silver nanoparticles (AgNPs) that simultaneously possess high stability and enhanced applicability.
32919574	6	38	theme	industrial	1136:1145	arg1	applications					1147:1158	industrial applications	1136:1158	industrial applications	1136:1158	The PVA-GG-AgNPs hydrogel composite exhibited excellent catalytic activity and antibacterial property, which makes them a suitable candidate for industrial applications.
32919574	6	39	theme	excellent	1037:1045	arg1	activity					1057:1064	excellent catalytic activity	1037:1064	excellent catalytic activity	1037:1064	The PVA-GG-AgNPs hydrogel composite exhibited excellent catalytic activity and antibacterial property, which makes them a suitable candidate for industrial applications.
32919574	3	40	theme	various	595:601	arg1	concentrations					603:616	various concentrations	595:616	various concentrations of the AgNO3 precursor solution	595:648	The AgNPs were synthesized in situ in the PVA-GG hydrogel from various concentrations of the AgNO3 precursor solution in the presence of NaBH4.
32919574	2	41	dep	AgNPs	379:383	arg1	composite					449:457	-guar gum (GG) smart hydrogel composite	419:457	-guar gum (GG) smart hydrogel composite	419:457	We report a rapid and efficient synthesis of AgNPs containing polyvinyl alcohol (PVA)-guar gum (GG) smart hydrogel composite, which exhibited pH-dependent swelling and enhanced mechanical strength.
32919574	1	42	theme	many	138:141	arg1	methods					155:161	many preparation methods	138:161	many preparation methods	138:161	Although many preparation methods have been reported till date, it is still a great challenge to prepare silver nanoparticles (AgNPs) that simultaneously possess high stability and enhanced applicability.
32919574	5	43	theme	swelling	909:916	arg1	capacity					918:925	swelling capacity	909:925	swelling capacity	909:925	Simultaneously, at the optimum concentration of AgNO3 (0.01 M), the tensile strength and elongation at break were enhanced by 74 % and 11 %, and swelling capacity was increased by 18 % as compared to PVA-GG hydrogel (control).
32919574	1	44	theme	preparation	143:153	arg1	methods					155:161	many preparation methods	138:161	many preparation methods	138:161	Although many preparation methods have been reported till date, it is still a great challenge to prepare silver nanoparticles (AgNPs) that simultaneously possess high stability and enhanced applicability.
32919574	0	45	theme	network	69:75	arg1	hydrogel					77:84	dual network hydrogel	64:84	dual network hydrogel for catalytic and biomedical applications	64:126	Highly stable silver nanoparticles containing guar gum modified dual network hydrogel for catalytic and biomedical applications.
32919574	5	46	theme	PVA-GG	964:969	arg1	control					981:987	control	981:987	control	981:987	Simultaneously, at the optimum concentration of AgNO3 (0.01 M), the tensile strength and elongation at break were enhanced by 74 % and 11 %, and swelling capacity was increased by 18 % as compared to PVA-GG hydrogel (control).
32919574	5	46	theme	PVA-GG	964:969	arg1	hydrogel					971:978	PVA-GG hydrogel	964:978	PVA-GG hydrogel (control)	964:988	Simultaneously, at the optimum concentration of AgNO3 (0.01 M), the tensile strength and elongation at break were enhanced by 74 % and 11 %, and swelling capacity was increased by 18 % as compared to PVA-GG hydrogel (control).
32919574	6	47	theme	suitable	1113:1120	arg1	candidate					1122:1130	a suitable candidate	1111:1130	a suitable candidate for industrial applications	1111:1158	The PVA-GG-AgNPs hydrogel composite exhibited excellent catalytic activity and antibacterial property, which makes them a suitable candidate for industrial applications.
32919574	6	48	theme	hydrogel	1008:1015	arg1	composite					1017:1025	The PVA-GG-AgNPs hydrogel composite	991:1025	The PVA-GG-AgNPs hydrogel composite	991:1025	The PVA-GG-AgNPs hydrogel composite exhibited excellent catalytic activity and antibacterial property, which makes them a suitable candidate for industrial applications.
32919574	0	49	theme	dual	64:67	arg1	hydrogel					77:84	dual network hydrogel	64:84	dual network hydrogel for catalytic and biomedical applications	64:126	Highly stable silver nanoparticles containing guar gum modified dual network hydrogel for catalytic and biomedical applications.
32919574	2	50	theme	-guar	419:423	arg1	composite					449:457	-guar gum (GG) smart hydrogel composite	419:457	-guar gum (GG) smart hydrogel composite	419:457	We report a rapid and efficient synthesis of AgNPs containing polyvinyl alcohol (PVA)-guar gum (GG) smart hydrogel composite, which exhibited pH-dependent swelling and enhanced mechanical strength.
32919574	2	51	theme	GG	430:431	arg1	composite					449:457	-guar gum (GG) smart hydrogel composite	419:457	-guar gum (GG) smart hydrogel composite	419:457	We report a rapid and efficient synthesis of AgNPs containing polyvinyl alcohol (PVA)-guar gum (GG) smart hydrogel composite, which exhibited pH-dependent swelling and enhanced mechanical strength.
32919574	1	52	theme	silver	234:239	arg1	AgNPs					256:260	AgNPs	256:260	AgNPs	256:260	Although many preparation methods have been reported till date, it is still a great challenge to prepare silver nanoparticles (AgNPs) that simultaneously possess high stability and enhanced applicability.
32919574	1	52	theme	silver	234:239	arg1	nanoparticles					241:253	silver nanoparticles	234:253	silver nanoparticles (AgNPs) that simultaneously possess high stability and enhanced applicability	234:331	Although many preparation methods have been reported till date, it is still a great challenge to prepare silver nanoparticles (AgNPs) that simultaneously possess high stability and enhanced applicability.
32495470	7	0	theme	compressive	1253:1263	arg1	strength					1265:1272	higher compressive strength	1246:1272	higher compressive strength	1246:1272	However, higher compressive strength was obtained for scaffolds cross-linked with STMP signifying that they have a better ability to resist compressive forces.
32495470	8	1	located	observed	1425:1432	arg1	scaffolds					1454:1462	STMP-crosslinked scaffolds	1437:1462	STMP-crosslinked scaffolds	1437:1462	Superior cell viability was observed in STMP-crosslinked scaffolds.
32495470	8	1	located	observed	1425:1432	arg2	viability					1411:1419	Superior cell viability	1397:1419	Superior cell viability	1397:1419	Superior cell viability was observed in STMP-crosslinked scaffolds.
32495470	0	2	theme	gum-based	16:24	arg1	scaffolds					36:44	gum-based composite scaffolds	16:44	gum-based composite scaffolds	16:44	Crosslinking of gum-based composite scaffolds for enhanced strength and stability: A comparative study between sodium trimetaphosphate and glutaraldehyde.
32495470	5	3	dep	in	1098:1099	arg1	vitro					1101:1105	vitro	1101:1105	vitro	1101:1105	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP) were fabricated to study the efficiency of crosslinkers and were characterized for degradation profile, swelling capacity, porosity, mechanical strength, morphology, X-ray diffraction, Fourier-transform infrared, and in vitro biocompatibility.
32495470	5	4	theme	crosslinkers	924:935	arg1	efficiency					910:919	the efficiency	906:919	the efficiency of crosslinkers	906:935	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP) were fabricated to study the efficiency of crosslinkers and were characterized for degradation profile, swelling capacity, porosity, mechanical strength, morphology, X-ray diffraction, Fourier-transform infrared, and in vitro biocompatibility.
32495470	5	5	theme	degradation	964:974	arg1	profile					976:982	degradation profile	964:982	degradation profile	964:982	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP) were fabricated to study the efficiency of crosslinkers and were characterized for degradation profile, swelling capacity, porosity, mechanical strength, morphology, X-ray diffraction, Fourier-transform infrared, and in vitro biocompatibility.
32495470	7	6	theme	compressive	1377:1387	arg1	forces					1389:1394	compressive forces	1377:1394	compressive forces	1377:1394	However, higher compressive strength was obtained for scaffolds cross-linked with STMP signifying that they have a better ability to resist compressive forces.
32495470	8	7	theme	Superior	1397:1404	arg1	viability					1411:1419	Superior cell viability	1397:1419	Superior cell viability	1397:1419	Superior cell viability was observed in STMP-crosslinked scaffolds.
32495470	2	8	theme	conventional	302:313	arg1	use					315:317	the conventional use	298:317	the conventional use of bone grafts	298:332	Engineered scaffolds are an excellent substitute for the conventional use of bone grafts as they are biocompatible, economic, and provide limitless supply with no risk of disease transmission.
32495470	4	9	theme	biodegradability	682:697	arg1	nature					699:704	biodegradability nature	682:704	biodegradability nature	682:704	Uniform blending of the gums and the presence of the optimal concentration of appropriate crosslinkers are very crucial for biodegradability nature.
32495470	0	10	theme	comparative	85:95	arg1	study					97:101	A comparative study	83:101	Crosslinking of gum-based composite scaffolds for enhanced strength and stability: A comparative study between sodium trimetaphosphate and glutaraldehyde.	0:153	Crosslinking of gum-based composite scaffolds for enhanced strength and stability: A comparative study between sodium trimetaphosphate and glutaraldehyde.
32495470	9	11	theme	bone	1588:1591	arg1	engineering					1600:1610	bone tissue engineering	1588:1610	bone tissue engineering	1588:1610	In conclusion, STMP serves as a better crosslinker in comparison to GA and can be used in the fabrication of scaffolds for bone tissue engineering.
32495470	9	12	used	used	1547:1550	arg2	STMP					1480:1483	STMP	1480:1483	STMP	1480:1483	In conclusion, STMP serves as a better crosslinker in comparison to GA and can be used in the fabrication of scaffolds for bone tissue engineering.
32495470	9	12	used	used	1547:1550	arg2	crosslinker					1504:1514	a better crosslinker	1495:1514	a better crosslinker in comparison to GA	1495:1534	In conclusion, STMP serves as a better crosslinker in comparison to GA and can be used in the fabrication of scaffolds for bone tissue engineering.
32495470	0	13	theme	sodium	111:116	arg1	trimetaphosphate					118:133	sodium trimetaphosphate	111:133	sodium trimetaphosphate	111:133	Crosslinking of gum-based composite scaffolds for enhanced strength and stability: A comparative study between sodium trimetaphosphate and glutaraldehyde.
32495470	4	14	theme	crosslinkers	648:659	arg1	concentration					619:631	the optimal concentration	607:631	the optimal concentration of appropriate crosslinkers	607:659	Uniform blending of the gums and the presence of the optimal concentration of appropriate crosslinkers are very crucial for biodegradability nature.
32495470	9	15	theme	tissue	1593:1598	arg1	engineering					1600:1610	bone tissue engineering	1588:1610	bone tissue engineering	1588:1610	In conclusion, STMP serves as a better crosslinker in comparison to GA and can be used in the fabrication of scaffolds for bone tissue engineering.
32495470	2	16	dep	excellent	273:281	arg1	substitute					283:292	substitute	283:292	substitute for the conventional use of bone grafts as they are biocompatible, economic, and provide limitless supply with no risk of disease transmission	283:435	Engineered scaffolds are an excellent substitute for the conventional use of bone grafts as they are biocompatible, economic, and provide limitless supply with no risk of disease transmission.
32495470	3	17	theme	Gum-based	438:446	arg1	scaffolds					448:456	Gum-based scaffolds	438:456	Gum-based scaffolds	438:456	Gum-based scaffolds present a good scope for studying tissue-engineering models and analyzing controlled drug delivery.
32495470	3	18	theme	good	468:471	arg1	scope					473:477	a good scope	466:477	a good scope for studying tissue-engineering models and analyzing controlled drug delivery	466:555	Gum-based scaffolds present a good scope for studying tissue-engineering models and analyzing controlled drug delivery.
32495470	8	19	theme	cell	1406:1409	arg1	viability					1411:1419	Superior cell viability	1397:1419	Superior cell viability	1397:1419	Superior cell viability was observed in STMP-crosslinked scaffolds.
32495470	4	20	theme	optimal	611:617	arg1	concentration					619:631	the optimal concentration	607:631	the optimal concentration of appropriate crosslinkers	607:659	Uniform blending of the gums and the presence of the optimal concentration of appropriate crosslinkers are very crucial for biodegradability nature.
32495470	9	21	theme	better	1497:1502	arg1	crosslinker					1504:1514	a better crosslinker	1495:1514	a better crosslinker in comparison to GA	1495:1534	In conclusion, STMP serves as a better crosslinker in comparison to GA and can be used in the fabrication of scaffolds for bone tissue engineering.
32495470	9	21	theme	better	1497:1502	arg1	STMP					1480:1483	STMP	1480:1483	STMP	1480:1483	In conclusion, STMP serves as a better crosslinker in comparison to GA and can be used in the fabrication of scaffolds for bone tissue engineering.
32495470	2	22	theme	transmission	424:435	arg1	risk					408:411	no risk	405:411	no risk of disease transmission	405:435	Engineered scaffolds are an excellent substitute for the conventional use of bone grafts as they are biocompatible, economic, and provide limitless supply with no risk of disease transmission.
32495470	2	23	theme	grafts	327:332	arg1	use					315:317	the conventional use	298:317	the conventional use of bone grafts	298:332	Engineered scaffolds are an excellent substitute for the conventional use of bone grafts as they are biocompatible, economic, and provide limitless supply with no risk of disease transmission.
32495470	2	24	theme	limitless	383:391	arg1	supply					393:398	limitless supply	383:398	limitless supply	383:398	Engineered scaffolds are an excellent substitute for the conventional use of bone grafts as they are biocompatible, economic, and provide limitless supply with no risk of disease transmission.
32495470	2	25	theme	bone	322:325	arg1	grafts					327:332	bone grafts	322:332	bone grafts	322:332	Engineered scaffolds are an excellent substitute for the conventional use of bone grafts as they are biocompatible, economic, and provide limitless supply with no risk of disease transmission.
32495470	1	26	theme	fields	198:203	arg1	fields					198:203	the potential fields	184:203	the potential fields in the domain of regenerative medicine	184:242	Tissue engineering is one of the potential fields in the domain of regenerative medicine.
32495470	1	26	theme	fields	198:203	arg1	one					177:179	one	177:179	one	177:179	Tissue engineering is one of the potential fields in the domain of regenerative medicine.
32495470	0	27	theme	composite	26:34	arg1	scaffolds					36:44	gum-based composite scaffolds	16:44	gum-based composite scaffolds	16:44	Crosslinking of gum-based composite scaffolds for enhanced strength and stability: A comparative study between sodium trimetaphosphate and glutaraldehyde.
32495470	2	28	dep	biocompatible	346:358	arg1	economic					361:368	economic	361:368	economic	361:368	Engineered scaffolds are an excellent substitute for the conventional use of bone grafts as they are biocompatible, economic, and provide limitless supply with no risk of disease transmission.
32495470	2	29	theme	disease	416:422	arg1	transmission					424:435	disease transmission	416:435	disease transmission	416:435	Engineered scaffolds are an excellent substitute for the conventional use of bone grafts as they are biocompatible, economic, and provide limitless supply with no risk of disease transmission.
32495470	1	30	theme	potential	188:196	arg1	fields					198:203	the potential fields	184:203	the potential fields in the domain of regenerative medicine	184:242	Tissue engineering is one of the potential fields in the domain of regenerative medicine.
32495470	4	31	theme	concentration	619:631	arg1	crucial					670:676	crucial	670:676	crucial	670:676	Uniform blending of the gums and the presence of the optimal concentration of appropriate crosslinkers are very crucial for biodegradability nature.
32495470	4	31	theme	concentration	619:631	arg1	blending					566:573	Uniform blending	558:573	Uniform blending of the gums	558:585	Uniform blending of the gums and the presence of the optimal concentration of appropriate crosslinkers are very crucial for biodegradability nature.
32495470	4	31	theme	concentration	619:631	arg1	presence					595:602	the presence	591:602	the presence of the optimal concentration of appropriate crosslinkers	591:659	Uniform blending of the gums and the presence of the optimal concentration of appropriate crosslinkers are very crucial for biodegradability nature.
32495470	5	32	theme	in	1098:1099	arg1	biocompatibility					1107:1122	in vitro biocompatibility	1098:1122	in vitro biocompatibility	1098:1122	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP) were fabricated to study the efficiency of crosslinkers and were characterized for degradation profile, swelling capacity, porosity, mechanical strength, morphology, X-ray diffraction, Fourier-transform infrared, and in vitro biocompatibility.
32495470	5	33	theme	swelling	985:992	arg1	capacity					994:1001	swelling capacity	985:1001	swelling capacity	985:1001	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP) were fabricated to study the efficiency of crosslinkers and were characterized for degradation profile, swelling capacity, porosity, mechanical strength, morphology, X-ray diffraction, Fourier-transform infrared, and in vitro biocompatibility.
32495470	9	34	theme	scaffolds	1574:1582	arg1	fabrication					1559:1569	the fabrication	1555:1569	the fabrication of scaffolds for bone tissue engineering	1555:1610	In conclusion, STMP serves as a better crosslinker in comparison to GA and can be used in the fabrication of scaffolds for bone tissue engineering.
32495470	3	35	theme	controlled	532:541	arg1	delivery					548:555	controlled drug delivery	532:555	controlled drug delivery	532:555	Gum-based scaffolds present a good scope for studying tissue-engineering models and analyzing controlled drug delivery.
32495470	4	36	theme	gums	582:585	arg1	crucial					670:676	crucial	670:676	crucial	670:676	Uniform blending of the gums and the presence of the optimal concentration of appropriate crosslinkers are very crucial for biodegradability nature.
32495470	4	36	theme	gums	582:585	arg1	blending					566:573	Uniform blending	558:573	Uniform blending of the gums	558:585	Uniform blending of the gums and the presence of the optimal concentration of appropriate crosslinkers are very crucial for biodegradability nature.
32495470	4	36	theme	gums	582:585	arg1	presence					595:602	the presence	591:602	the presence of the optimal concentration of appropriate crosslinkers	591:659	Uniform blending of the gums and the presence of the optimal concentration of appropriate crosslinkers are very crucial for biodegradability nature.
32495470	5	37	theme	xanthan	750:756	arg1	gum					758:760	xanthan gum	750:760	xanthan gum	750:760	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP) were fabricated to study the efficiency of crosslinkers and were characterized for degradation profile, swelling capacity, porosity, mechanical strength, morphology, X-ray diffraction, Fourier-transform infrared, and in vitro biocompatibility.
32495470	7	38	contain	have	1345:1348	arg2	ability					1359:1365	a better ability	1350:1365	a better ability to resist compressive forces	1350:1394	However, higher compressive strength was obtained for scaffolds cross-linked with STMP signifying that they have a better ability to resist compressive forces.
32495470	7	38	contain	have	1345:1348	arg1	they					1340:1343	they	1340:1343	they	1340:1343	However, higher compressive strength was obtained for scaffolds cross-linked with STMP signifying that they have a better ability to resist compressive forces.
32495470	2	39	theme	Engineered	245:254	arg1	scaffolds					256:264	Engineered scaffolds	245:264	Engineered scaffolds	245:264	Engineered scaffolds are an excellent substitute for the conventional use of bone grafts as they are biocompatible, economic, and provide limitless supply with no risk of disease transmission.
32495470	2	39	theme	Engineered	245:254	arg1	excellent					273:281	excellent	273:281	excellent	273:281	Engineered scaffolds are an excellent substitute for the conventional use of bone grafts as they are biocompatible, economic, and provide limitless supply with no risk of disease transmission.
32495470	0	40	theme	scaffolds	36:44	arg1	Crosslinking					0:11	Crosslinking	0:11	Crosslinking of gum-based composite scaffolds for enhanced strength and stability: A comparative study between sodium trimetaphosphate and glutaraldehyde.	0:153	Crosslinking of gum-based composite scaffolds for enhanced strength and stability: A comparative study between sodium trimetaphosphate and glutaraldehyde.
32495470	8	41	link	STMP-crosslinked	1437:1452	arg1	scaffolds					1454:1462	STMP-crosslinked scaffolds	1437:1462	STMP-crosslinked scaffolds	1437:1462	Superior cell viability was observed in STMP-crosslinked scaffolds.
32495470	5	42	theme	X-ray	1047:1051	arg1	diffraction					1053:1063	X-ray diffraction	1047:1063	X-ray diffraction	1047:1063	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP) were fabricated to study the efficiency of crosslinkers and were characterized for degradation profile, swelling capacity, porosity, mechanical strength, morphology, X-ray diffraction, Fourier-transform infrared, and in vitro biocompatibility.
32495470	5	43	contain	containing	727:736	arg2	gum					758:760	xanthan gum	750:760	xanthan gum	750:760	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP) were fabricated to study the efficiency of crosslinkers and were characterized for degradation profile, swelling capacity, porosity, mechanical strength, morphology, X-ray diffraction, Fourier-transform infrared, and in vitro biocompatibility.
32495470	5	43	contain	containing	727:736	arg2	hydroxyapatite					786:799	hydroxyapatite	786:799	hydroxyapatite	786:799	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP) were fabricated to study the efficiency of crosslinkers and were characterized for degradation profile, swelling capacity, porosity, mechanical strength, morphology, X-ray diffraction, Fourier-transform infrared, and in vitro biocompatibility.
32495470	5	43	contain	containing	727:736	arg2	alcohol					773:779	polyvinyl alcohol	763:779	polyvinyl alcohol	763:779	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP) were fabricated to study the efficiency of crosslinkers and were characterized for degradation profile, swelling capacity, porosity, mechanical strength, morphology, X-ray diffraction, Fourier-transform infrared, and in vitro biocompatibility.
32495470	5	43	contain	containing	727:736	arg1	scaffolds					717:725	Gum-based scaffolds	707:725	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP)	707:879	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP) were fabricated to study the efficiency of crosslinkers and were characterized for degradation profile, swelling capacity, porosity, mechanical strength, morphology, X-ray diffraction, Fourier-transform infrared, and in vitro biocompatibility.
32495470	5	43	contain	containing	727:736	arg2	gum					745:747	gellan gum	738:747	gellan gum	738:747	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP) were fabricated to study the efficiency of crosslinkers and were characterized for degradation profile, swelling capacity, porosity, mechanical strength, morphology, X-ray diffraction, Fourier-transform infrared, and in vitro biocompatibility.
32495470	5	44	theme	Gum-based	707:715	arg1	scaffolds					717:725	Gum-based scaffolds	707:725	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP)	707:879	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP) were fabricated to study the efficiency of crosslinkers and were characterized for degradation profile, swelling capacity, porosity, mechanical strength, morphology, X-ray diffraction, Fourier-transform infrared, and in vitro biocompatibility.
32495470	3	45	theme	tissue-engineering	492:509	arg1	models					511:516	tissue-engineering models	492:516	tissue-engineering models	492:516	Gum-based scaffolds present a good scope for studying tissue-engineering models and analyzing controlled drug delivery.
32495470	0	46	theme	enhanced	50:57	arg1	strength					59:66	strength	59:66	strength	59:66	Crosslinking of gum-based composite scaffolds for enhanced strength and stability: A comparative study between sodium trimetaphosphate and glutaraldehyde.
32495470	5	47	theme	Fourier-transform	1066:1082	arg1	infrared					1084:1091	Fourier-transform infrared	1066:1091	Fourier-transform infrared	1066:1091	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP) were fabricated to study the efficiency of crosslinkers and were characterized for degradation profile, swelling capacity, porosity, mechanical strength, morphology, X-ray diffraction, Fourier-transform infrared, and in vitro biocompatibility.
32495470	7	48	theme	higher	1246:1251	arg1	strength					1265:1272	higher compressive strength	1246:1272	higher compressive strength	1246:1272	However, higher compressive strength was obtained for scaffolds cross-linked with STMP signifying that they have a better ability to resist compressive forces.
32495470	6	49	theme	degradation	1174:1184	arg1	rate					1186:1189	higher degradation rate	1167:1189	higher degradation rate	1167:1189	Scaffolds crosslinked with STMP exhibited higher degradation rate at Day 21 than scaffolds crosslinked with GA.
32495470	4	50	theme	Uniform	558:564	arg1	blending					566:573	Uniform blending	558:573	Uniform blending of the gums	558:585	Uniform blending of the gums and the presence of the optimal concentration of appropriate crosslinkers are very crucial for biodegradability nature.
32495470	5	51	theme	mechanical	1014:1023	arg1	strength					1025:1032	mechanical strength	1014:1032	mechanical strength	1014:1032	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP) were fabricated to study the efficiency of crosslinkers and were characterized for degradation profile, swelling capacity, porosity, mechanical strength, morphology, X-ray diffraction, Fourier-transform infrared, and in vitro biocompatibility.
32495470	6	52	theme	higher	1167:1172	arg1	rate					1186:1189	higher degradation rate	1167:1189	higher degradation rate	1167:1189	Scaffolds crosslinked with STMP exhibited higher degradation rate at Day 21 than scaffolds crosslinked with GA.
32495470	8	53	theme	STMP-crosslinked	1437:1452	arg1	scaffolds					1454:1462	STMP-crosslinked scaffolds	1437:1462	STMP-crosslinked scaffolds	1437:1462	Superior cell viability was observed in STMP-crosslinked scaffolds.
32495470	1	54	theme	regenerative	222:233	arg1	medicine					235:242	regenerative medicine	222:242	regenerative medicine	222:242	Tissue engineering is one of the potential fields in the domain of regenerative medicine.
32495470	5	55	theme	gellan	738:743	arg1	gum					745:747	gellan gum	738:747	gellan gum	738:747	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP) were fabricated to study the efficiency of crosslinkers and were characterized for degradation profile, swelling capacity, porosity, mechanical strength, morphology, X-ray diffraction, Fourier-transform infrared, and in vitro biocompatibility.
32495470	5	56	theme	sodium	850:855	arg1	STMP					875:878	STMP	875:878	STMP	875:878	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP) were fabricated to study the efficiency of crosslinkers and were characterized for degradation profile, swelling capacity, porosity, mechanical strength, morphology, X-ray diffraction, Fourier-transform infrared, and in vitro biocompatibility.
32495470	5	56	theme	sodium	850:855	arg1	trimetaphosphate					857:872	sodium trimetaphosphate	850:872	sodium trimetaphosphate (STMP)	850:879	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP) were fabricated to study the efficiency of crosslinkers and were characterized for degradation profile, swelling capacity, porosity, mechanical strength, morphology, X-ray diffraction, Fourier-transform infrared, and in vitro biocompatibility.
32495470	9	57	from	crosslinker	1504:1514	arg1	comparison					1519:1528	comparison	1519:1528	comparison to GA	1519:1534	In conclusion, STMP serves as a better crosslinker in comparison to GA and can be used in the fabrication of scaffolds for bone tissue engineering.
32495470	1	58	theme	medicine	235:242	arg1	domain					212:217	the domain	208:217	the domain of regenerative medicine	208:242	Tissue engineering is one of the potential fields in the domain of regenerative medicine.
32495470	1	58	theme	medicine	235:242	arg1	medicine					235:242	regenerative medicine	222:242	regenerative medicine	222:242	Tissue engineering is one of the potential fields in the domain of regenerative medicine.
32495470	1	59	from	fields	198:203	arg1	domain					212:217	the domain	208:217	the domain of regenerative medicine	208:242	Tissue engineering is one of the potential fields in the domain of regenerative medicine.
32495470	1	59	from	fields	198:203	arg1	medicine					235:242	regenerative medicine	222:242	regenerative medicine	222:242	Tissue engineering is one of the potential fields in the domain of regenerative medicine.
32495470	1	60	theme	Tissue	155:160	arg1	engineering					162:172	Tissue engineering	155:172	Tissue engineering	155:172	Tissue engineering is one of the potential fields in the domain of regenerative medicine.
32495470	7	61	theme	better	1352:1357	arg1	ability					1359:1365	a better ability	1350:1365	a better ability to resist compressive forces	1350:1394	However, higher compressive strength was obtained for scaffolds cross-linked with STMP signifying that they have a better ability to resist compressive forces.
32495470	4	62	theme	appropriate	636:646	arg1	crosslinkers					648:659	appropriate crosslinkers	636:659	appropriate crosslinkers	636:659	Uniform blending of the gums and the presence of the optimal concentration of appropriate crosslinkers are very crucial for biodegradability nature.
32495470	0	63	dep	Crosslinking	0:11	arg1	study					97:101	A comparative study	83:101	Crosslinking of gum-based composite scaffolds for enhanced strength and stability: A comparative study between sodium trimetaphosphate and glutaraldehyde.	0:153	Crosslinking of gum-based composite scaffolds for enhanced strength and stability: A comparative study between sodium trimetaphosphate and glutaraldehyde.
32495470	3	64	theme	drug	543:546	arg1	delivery					548:555	controlled drug delivery	532:555	controlled drug delivery	532:555	Gum-based scaffolds present a good scope for studying tissue-engineering models and analyzing controlled drug delivery.
32495470	5	65	theme	polyvinyl	763:771	arg1	alcohol					773:779	polyvinyl alcohol	763:779	polyvinyl alcohol	763:779	Gum-based scaffolds containing gellan gum, xanthan gum, polyvinyl alcohol, and hydroxyapatite, cross-linked with either glutaraldehyde (GA) or sodium trimetaphosphate (STMP) were fabricated to study the efficiency of crosslinkers and were characterized for degradation profile, swelling capacity, porosity, mechanical strength, morphology, X-ray diffraction, Fourier-transform infrared, and in vitro biocompatibility.
33594917	5	0	theme	wound	742:746	arg1	composite					758:766	the wound bandaging composite	738:766	the wound bandaging composite	738:766	Additionally, glycerol was added to the Ce-nGels, and the resulting compositions (Ce-nGel-Glu) were coated on cotton fabrics to generate the wound bandaging composite.
33594917	4	1	theme	nanogel	484:490	arg1	sizes					501:505	The nanogel particle sizes	480:505	The nanogel particle sizes	480:505	The nanogel particle sizes were evaluated by transmission electron microscopy and determined to range from ∼10 to 50 nm.
33594917	9	2	theme	rapid	1118:1122	arg1	healing					1124:1130	rapid healing	1118:1130	rapid healing	1118:1130	In contrast to the untreated wounds, rapid healing was perceived in the Ce-nGel-Glu-treated wound with less damage.
33594917	0	3	theme	care	87:90	arg1	systems					92:98	wound healing care systems	73:98	wound healing care systems	73:98	Cerium oxide nanoparticle-loaded polyvinyl alcohol nanogels delivery for wound healing care systems on surgery.
33594917	6	4	theme	total	864:868	arg1	loading					870:876	the total loading	860:876	the total loading	860:876	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	3	5	from	influences	390:399	arg1	formations					454:463	PVA nanogel formations	442:463	PVA nanogel formations	442:463	The influences of different experimental limitations on PVA nanogel formations were examined.
33594917	5	6	theme	cotton	711:716	arg1	fabrics					718:724	cotton fabrics	711:724	cotton fabrics	711:724	Additionally, glycerol was added to the Ce-nGels, and the resulting compositions (Ce-nGel-Glu) were coated on cotton fabrics to generate the wound bandaging composite.
33594917	6	7	theme	release	789:795	arg1	%					855:855	∼38%	852:855	∼38% of the total loading	852:876	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	6	7	theme	release	789:795	arg1	loading					870:876	the total loading	860:876	the total loading	860:876	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	6	7	theme	release	789:795	arg1	profile					797:803	The cumulative drug release profile	769:803	The cumulative drug release profile of the Cerium from the bandage	769:834	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	2	8	theme	CeNP	264:267	arg1	Ce-nGel					278:284	Ce-nGel	278:284	Ce-nGel	278:284	The CeNP nanogel (Ce-nGel) was fabricated by the fructose-mediated reduction of Cerium oxide solutions within the PVA matrix.
33594917	2	8	theme	CeNP	264:267	arg1	nanogel					269:275	The CeNP nanogel	260:275	The CeNP nanogel (Ce-nGel)	260:285	The CeNP nanogel (Ce-nGel) was fabricated by the fructose-mediated reduction of Cerium oxide solutions within the PVA matrix.
33594917	5	9	theme	bandaging	748:756	arg1	composite					758:766	the wound bandaging composite	738:766	the wound bandaging composite	738:766	Additionally, glycerol was added to the Ce-nGels, and the resulting compositions (Ce-nGel-Glu) were coated on cotton fabrics to generate the wound bandaging composite.
33594917	6	10	theme	drug	784:787	arg1	%					855:855	∼38%	852:855	∼38% of the total loading	852:876	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	6	10	theme	drug	784:787	arg1	loading					870:876	the total loading	860:876	the total loading	860:876	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	6	10	theme	drug	784:787	arg1	profile					797:803	The cumulative drug release profile	769:803	The cumulative drug release profile of the Cerium from the bandage	769:834	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	1	11	theme	wound	168:172	arg1	bandages					174:181	wound bandages	168:181	wound bandages	168:181	This study was designed to establish the composition of wound bandages based on Cerium nanoparticle (CeNP)-loaded polyvinyl alcohol (PVA) nanogels.
33594917	8	12	theme	in vivo	1013:1019	arg1	healing					1021:1027	in vivo healing	1013:1027	in vivo healing of skin wounds formed in mouse models over 24 days	1013:1078	Moreover, we examined in vivo healing of skin wounds formed in mouse models over 24 days.
33594917	6	13	theme	cumulative	773:782	arg1	%					855:855	∼38%	852:855	∼38% of the total loading	852:876	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	6	13	theme	cumulative	773:782	arg1	loading					870:876	the total loading	860:876	the total loading	860:876	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	6	13	theme	cumulative	773:782	arg1	profile					797:803	The cumulative drug release profile	769:803	The cumulative drug release profile of the Cerium from the bandage	769:834	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	6	14	from	bandage	828:834	arg1	%					855:855	∼38%	852:855	∼38% of the total loading	852:876	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	6	14	from	bandage	828:834	arg1	loading					870:876	the total loading	860:876	the total loading	860:876	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	6	14	from	bandage	828:834	arg1	profile					797:803	The cumulative drug release profile	769:803	The cumulative drug release profile of the Cerium from the bandage	769:834	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	6	14	from	bandage	828:834	arg1	Cerium					812:817	the Cerium	808:817	the Cerium from the bandage	808:834	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	3	15	theme	limitations	427:437	arg1	influences					390:399	The influences	386:399	The influences of different experimental limitations on PVA nanogel formations	386:463	The influences of different experimental limitations on PVA nanogel formations were examined.
33594917	0	16	theme	oxide	7:11	arg1	alcohol					43:49	Cerium oxide nanoparticle-loaded polyvinyl alcohol	0:49	Cerium oxide nanoparticle-loaded polyvinyl alcohol	0:49	Cerium oxide nanoparticle-loaded polyvinyl alcohol nanogels delivery for wound healing care systems on surgery.
33594917	4	17	from	50 nm	594:598	arg1	range					576:580	range	576:580	range from ∼10 to 50 nm	576:598	The nanogel particle sizes were evaluated by transmission electron microscopy and determined to range from ∼10 to 50 nm.
33594917	8	18	theme	skin	1032:1035	arg1	wounds					1037:1042	skin wounds	1032:1042	skin wounds formed in mouse models over 24 days	1032:1078	Moreover, we examined in vivo healing of skin wounds formed in mouse models over 24 days.
33594917	6	19	from	profile	797:803	arg1	bandage					828:834	the bandage	824:834	the bandage	824:834	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	10	20	theme	bandaging	1244:1252	arg1	candidate					1285:1293	a potential candidate	1273:1293	a potential candidate for wound healing applications	1273:1324	These findings indicate that Ce-nGel-Glu-based bandaging materials could be a potential candidate for wound healing applications in the future.
33594917	10	20	theme	bandaging	1244:1252	arg1	materials					1254:1262	Ce-nGel-Glu-based bandaging materials	1226:1262	Ce-nGel-Glu-based bandaging materials	1226:1262	These findings indicate that Ce-nGel-Glu-based bandaging materials could be a potential candidate for wound healing applications in the future.
33594917	0	21	theme	Cerium	0:5	arg1	alcohol					43:49	Cerium oxide nanoparticle-loaded polyvinyl alcohol	0:49	Cerium oxide nanoparticle-loaded polyvinyl alcohol	0:49	Cerium oxide nanoparticle-loaded polyvinyl alcohol nanogels delivery for wound healing care systems on surgery.
33594917	9	22	with	wound	1173:1177	arg1	damage					1189:1194	less damage	1184:1194	less damage	1184:1194	In contrast to the untreated wounds, rapid healing was perceived in the Ce-nGel-Glu-treated wound with less damage.
33594917	3	23	theme	different	404:412	arg1	limitations					427:437	different experimental limitations	404:437	different experimental limitations	404:437	The influences of different experimental limitations on PVA nanogel formations were examined.
33594917	0	24	theme	polyvinyl	33:41	arg1	alcohol					43:49	Cerium oxide nanoparticle-loaded polyvinyl alcohol	0:49	Cerium oxide nanoparticle-loaded polyvinyl alcohol	0:49	Cerium oxide nanoparticle-loaded polyvinyl alcohol nanogels delivery for wound healing care systems on surgery.
33594917	9	25	theme	untreated	1100:1108	arg1	wounds					1110:1115	the untreated wounds	1096:1115	the untreated wounds	1096:1115	In contrast to the untreated wounds, rapid healing was perceived in the Ce-nGel-Glu-treated wound with less damage.
33594917	7	26	theme	antibacterial	908:920	arg1	efficacy					922:929	antibacterial efficacy	908:929	antibacterial efficacy	908:929	Additionally, antibacterial efficacy was developed for Gam positive and negative microorganisms.
33594917	10	27	theme	potential	1275:1283	arg1	candidate					1285:1293	a potential candidate	1273:1293	a potential candidate for wound healing applications	1273:1324	These findings indicate that Ce-nGel-Glu-based bandaging materials could be a potential candidate for wound healing applications in the future.
33594917	10	27	theme	potential	1275:1283	arg1	materials					1254:1262	Ce-nGel-Glu-based bandaging materials	1226:1262	Ce-nGel-Glu-based bandaging materials	1226:1262	These findings indicate that Ce-nGel-Glu-based bandaging materials could be a potential candidate for wound healing applications in the future.
33594917	3	28	theme	nanogel	446:452	arg1	formations					454:463	PVA nanogel formations	442:463	PVA nanogel formations	442:463	The influences of different experimental limitations on PVA nanogel formations were examined.
33594917	0	29	theme	nanoparticle-loaded	13:31	arg1	alcohol					43:49	Cerium oxide nanoparticle-loaded polyvinyl alcohol	0:49	Cerium oxide nanoparticle-loaded polyvinyl alcohol	0:49	Cerium oxide nanoparticle-loaded polyvinyl alcohol nanogels delivery for wound healing care systems on surgery.
33594917	8	30	theme	wounds	1037:1042	arg1	healing					1021:1027	in vivo healing	1013:1027	in vivo healing of skin wounds formed in mouse models over 24 days	1013:1078	Moreover, we examined in vivo healing of skin wounds formed in mouse models over 24 days.
33594917	9	31	theme	less	1184:1187	arg1	damage					1189:1194	less damage	1184:1194	less damage	1184:1194	In contrast to the untreated wounds, rapid healing was perceived in the Ce-nGel-Glu-treated wound with less damage.
33594917	9	32	dep	wounds	1110:1115	arg1	contrast					1084:1091	contrast	1084:1091	contrast	1084:1091	In contrast to the untreated wounds, rapid healing was perceived in the Ce-nGel-Glu-treated wound with less damage.
33594917	6	33	theme	loading	870:876	arg1	%					855:855	∼38%	852:855	∼38% of the total loading	852:876	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	6	33	theme	loading	870:876	arg1	profile					797:803	The cumulative drug release profile	769:803	The cumulative drug release profile of the Cerium from the bandage	769:834	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	6	33	theme	loading	870:876	arg1	loading					870:876	the total loading	860:876	the total loading	860:876	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	8	34	theme	mouse	1054:1058	arg1	models					1060:1065	mouse models	1054:1065	mouse models	1054:1065	Moreover, we examined in vivo healing of skin wounds formed in mouse models over 24 days.
33594917	2	35	theme	solutions	353:361	arg1	reduction					327:335	the fructose-mediated reduction	305:335	the fructose-mediated reduction of Cerium oxide solutions within the PVA matrix	305:383	The CeNP nanogel (Ce-nGel) was fabricated by the fructose-mediated reduction of Cerium oxide solutions within the PVA matrix.
33594917	2	36	theme	PVA	374:376	arg1	matrix					378:383	the PVA matrix	370:383	the PVA matrix	370:383	The CeNP nanogel (Ce-nGel) was fabricated by the fructose-mediated reduction of Cerium oxide solutions within the PVA matrix.
33594917	3	37	theme	PVA	442:444	arg1	formations					454:463	PVA nanogel formations	442:463	PVA nanogel formations	442:463	The influences of different experimental limitations on PVA nanogel formations were examined.
33594917	9	38	theme	Ce-nGel-Glu-treated	1153:1171	arg1	wound					1173:1177	the Ce-nGel-Glu-treated wound	1149:1177	the Ce-nGel-Glu-treated wound with less damage	1149:1194	In contrast to the untreated wounds, rapid healing was perceived in the Ce-nGel-Glu-treated wound with less damage.
33594917	3	39	theme	experimental	414:425	arg1	limitations					427:437	different experimental limitations	404:437	different experimental limitations	404:437	The influences of different experimental limitations on PVA nanogel formations were examined.
33594917	2	40	theme	fructose-mediated	309:325	arg1	reduction					327:335	the fructose-mediated reduction	305:335	the fructose-mediated reduction of Cerium oxide solutions within the PVA matrix	305:383	The CeNP nanogel (Ce-nGel) was fabricated by the fructose-mediated reduction of Cerium oxide solutions within the PVA matrix.
33594917	4	41	theme	electron	538:545	arg1	microscopy					547:556	transmission electron microscopy	525:556	transmission electron microscopy	525:556	The nanogel particle sizes were evaluated by transmission electron microscopy and determined to range from ∼10 to 50 nm.
33594917	5	42	theme	resulting	659:667	arg1	compositions					669:680	the resulting compositions	655:680	the resulting compositions (Ce-nGel-Glu)	655:694	Additionally, glycerol was added to the Ce-nGels, and the resulting compositions (Ce-nGel-Glu) were coated on cotton fabrics to generate the wound bandaging composite.
33594917	5	42	theme	resulting	659:667	arg1	Ce-nGel-Glu					683:693	Ce-nGel-Glu	683:693	Ce-nGel-Glu	683:693	Additionally, glycerol was added to the Ce-nGels, and the resulting compositions (Ce-nGel-Glu) were coated on cotton fabrics to generate the wound bandaging composite.
33594917	10	43	theme	healing	1305:1311	arg1	applications					1313:1324	wound healing applications	1299:1324	wound healing applications	1299:1324	These findings indicate that Ce-nGel-Glu-based bandaging materials could be a potential candidate for wound healing applications in the future.
33594917	4	44	theme	transmission	525:536	arg1	microscopy					547:556	transmission electron microscopy	525:556	transmission electron microscopy	525:556	The nanogel particle sizes were evaluated by transmission electron microscopy and determined to range from ∼10 to 50 nm.
33594917	7	45	theme	negative	966:973	arg1	microorganisms					975:988	Gam positive and negative microorganisms	949:988	Gam positive and negative microorganisms	949:988	Additionally, antibacterial efficacy was developed for Gam positive and negative microorganisms.
33594917	10	46	theme	wound	1299:1303	arg1	healing					1305:1311	wound healing	1299:1311	wound healing applications	1299:1324	These findings indicate that Ce-nGel-Glu-based bandaging materials could be a potential candidate for wound healing applications in the future.
33594917	6	47	theme	Cerium	812:817	arg1	%					855:855	∼38%	852:855	∼38% of the total loading	852:876	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	6	47	theme	Cerium	812:817	arg1	loading					870:876	the total loading	860:876	the total loading	860:876	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	6	47	theme	Cerium	812:817	arg1	profile					797:803	The cumulative drug release profile	769:803	The cumulative drug release profile of the Cerium from the bandage	769:834	The cumulative drug release profile of the Cerium from the bandage was found to be ∼38% of the total loading after two days.
33594917	7	48	theme	Gam	949:951	arg1	microorganisms					975:988	Gam positive and negative microorganisms	949:988	Gam positive and negative microorganisms	949:988	Additionally, antibacterial efficacy was developed for Gam positive and negative microorganisms.
33594917	1	49	theme	-loaded	218:224	arg1	nanogels					250:257	Cerium nanoparticle (CeNP)-loaded polyvinyl alcohol (PVA) nanogels	192:257	Cerium nanoparticle (CeNP)-loaded polyvinyl alcohol (PVA) nanogels	192:257	This study was designed to establish the composition of wound bandages based on Cerium nanoparticle (CeNP)-loaded polyvinyl alcohol (PVA) nanogels.
33594917	0	50	theme	healing	79:85	arg1	systems					92:98	wound healing care systems	73:98	wound healing care systems	73:98	Cerium oxide nanoparticle-loaded polyvinyl alcohol nanogels delivery for wound healing care systems on surgery.
33594917	4	51	dep	50 nm	594:598	arg1	to					591:592	to	591:592	to	591:592	The nanogel particle sizes were evaluated by transmission electron microscopy and determined to range from ∼10 to 50 nm.
33594917	7	52	theme	positive	953:960	arg1	microorganisms					975:988	Gam positive and negative microorganisms	949:988	Gam positive and negative microorganisms	949:988	Additionally, antibacterial efficacy was developed for Gam positive and negative microorganisms.
33594917	2	53	theme	oxide	347:351	arg1	solutions					353:361	Cerium oxide solutions	340:361	Cerium oxide solutions	340:361	The CeNP nanogel (Ce-nGel) was fabricated by the fructose-mediated reduction of Cerium oxide solutions within the PVA matrix.
33594917	1	54	theme	bandages	174:181	arg1	composition					153:163	the composition	149:163	the composition of wound bandages based on Cerium nanoparticle (CeNP)-loaded polyvinyl alcohol (PVA) nanogels	149:257	This study was designed to establish the composition of wound bandages based on Cerium nanoparticle (CeNP)-loaded polyvinyl alcohol (PVA) nanogels.
33594917	1	55	theme	polyvinyl	226:234	arg1	PVA					245:247	PVA	245:247	PVA	245:247	This study was designed to establish the composition of wound bandages based on Cerium nanoparticle (CeNP)-loaded polyvinyl alcohol (PVA) nanogels.
33594917	1	55	theme	polyvinyl	226:234	arg1	alcohol					236:242	polyvinyl alcohol	226:242	Cerium nanoparticle (CeNP)-loaded polyvinyl alcohol (PVA) nanogels	192:257	This study was designed to establish the composition of wound bandages based on Cerium nanoparticle (CeNP)-loaded polyvinyl alcohol (PVA) nanogels.
33594917	0	56	theme	wound	73:77	arg1	healing					79:85	wound healing	73:85	wound healing care systems	73:98	Cerium oxide nanoparticle-loaded polyvinyl alcohol nanogels delivery for wound healing care systems on surgery.
33594917	4	57	theme	particle	492:499	arg1	sizes					501:505	The nanogel particle sizes	480:505	The nanogel particle sizes	480:505	The nanogel particle sizes were evaluated by transmission electron microscopy and determined to range from ∼10 to 50 nm.
33594917	10	58	from	candidate	1285:1293	arg1	future					1333:1338	future	1333:1338	future	1333:1338	These findings indicate that Ce-nGel-Glu-based bandaging materials could be a potential candidate for wound healing applications in the future.
33594917	2	59	theme	Cerium	340:345	arg1	solutions					353:361	Cerium oxide solutions	340:361	Cerium oxide solutions	340:361	The CeNP nanogel (Ce-nGel) was fabricated by the fructose-mediated reduction of Cerium oxide solutions within the PVA matrix.
33594917	10	60	theme	Ce-nGel-Glu-based	1226:1242	arg1	candidate					1285:1293	a potential candidate	1273:1293	a potential candidate for wound healing applications	1273:1324	These findings indicate that Ce-nGel-Glu-based bandaging materials could be a potential candidate for wound healing applications in the future.
33594917	10	60	theme	Ce-nGel-Glu-based	1226:1242	arg1	materials					1254:1262	Ce-nGel-Glu-based bandaging materials	1226:1262	Ce-nGel-Glu-based bandaging materials	1226:1262	These findings indicate that Ce-nGel-Glu-based bandaging materials could be a potential candidate for wound healing applications in the future.
33594917	1	61	theme	alcohol	236:242	arg1	nanogels					250:257	Cerium nanoparticle (CeNP)-loaded polyvinyl alcohol (PVA) nanogels	192:257	Cerium nanoparticle (CeNP)-loaded polyvinyl alcohol (PVA) nanogels	192:257	This study was designed to establish the composition of wound bandages based on Cerium nanoparticle (CeNP)-loaded polyvinyl alcohol (PVA) nanogels.
33677972	0	0	theme	Oligosaccharides	80:95	arg1	Composition					122:132	the Fecal Microbiota Composition	101:132	the Fecal Microbiota Composition of Breastfed Infants	101:153	Maternal Fucosyltransferase 2 Status Associates with the Profiles of Human Milk Oligosaccharides and the Fecal Microbiota Composition of Breastfed Infants.
33677972	0	0	theme	Oligosaccharides	80:95	arg1	Profiles					57:64	the Profiles	53:64	the Profiles of Human Milk Oligosaccharides	53:95	Maternal Fucosyltransferase 2 Status Associates with the Profiles of Human Milk Oligosaccharides and the Fecal Microbiota Composition of Breastfed Infants.
33677972	1	1	theme	glycosyltransferase	313:331	arg1	phenotype					333:341	the mother's glycosyltransferase phenotype	300:341	the mother's glycosyltransferase phenotype	300:341	Human milk oligosaccharides (HMOs) play key roles in shaping infant fecal microbiota, and HMOs profiles have been reported to vary according to the mother's glycosyltransferase phenotype.
33677972	4	2	from	infants	788:794	arg1	enriched					776:783	enriched	776:783	enriched	776:783	The amount of total and fucosylated HMOs were higher in secretor than non-secretor mothers, while Bifidobacterium genus were highly enriched in infants fed by non-secretor mothers.
33677972	1	3	theme	milk	162:165	arg1	HMOs					185:188	HMOs	185:188	HMOs	185:188	Human milk oligosaccharides (HMOs) play key roles in shaping infant fecal microbiota, and HMOs profiles have been reported to vary according to the mother's glycosyltransferase phenotype.
33677972	1	3	theme	milk	162:165	arg1	oligosaccharides					167:182	Human milk oligosaccharides	156:182	Human milk oligosaccharides (HMOs)	156:189	Human milk oligosaccharides (HMOs) play key roles in shaping infant fecal microbiota, and HMOs profiles have been reported to vary according to the mother's glycosyltransferase phenotype.
33677972	6	4	from	strains	1300:1306	arg1	manner					1328:1333	species-specific manner	1311:1333	species-specific manner	1311:1333	The present study provides the HMO profiles from Chinese mothers and their associations with infant fecal microbiota composition, suggesting that HMO compositions are associated with different Bifidobacterium strains in species-specific manner.
33677972	0	5	theme	Milk	75:78	arg1	Oligosaccharides					80:95	Human Milk Oligosaccharides	69:95	Human Milk Oligosaccharides	69:95	Maternal Fucosyltransferase 2 Status Associates with the Profiles of Human Milk Oligosaccharides and the Fecal Microbiota Composition of Breastfed Infants.
33677972	2	6	from	mothers	424:430	arg1	milk					389:392	human milk	383:392	human milk from secretor or non-secretor mothers collected at 2 months postpartum	383:463	In this study, the profiles of HMOs in human milk from secretor or non-secretor mothers collected at 2 months postpartum were analyzed by liquid chromatography quadrupole time-of-flight tandem mass spectrometry.
33677972	2	6	from	mothers	424:430	arg1	profiles					363:370	the profiles	359:370	the profiles of HMOs in human milk from secretor or non-secretor mothers collected at 2 months postpartum	359:463	In this study, the profiles of HMOs in human milk from secretor or non-secretor mothers collected at 2 months postpartum were analyzed by liquid chromatography quadrupole time-of-flight tandem mass spectrometry.
33677972	6	7	theme	infant	1184:1189	arg1	composition					1208:1218	infant fecal microbiota composition	1184:1218	infant fecal microbiota composition	1184:1218	The present study provides the HMO profiles from Chinese mothers and their associations with infant fecal microbiota composition, suggesting that HMO compositions are associated with different Bifidobacterium strains in species-specific manner.
33677972	5	8	theme	Bifidobacterium-OTU158	917:938	arg1	abundance					904:912	the relative abundance	891:912	the relative abundance of Bifidobacterium-OTU158	891:938	Associations between HMOs and infant fecal microbiota showed that the relative abundance of Bifidobacterium-OTU158 was positively associated with 2'-fucosyllactose and 3-fucosyllactose, and Bifidobacterium-OTU90 was negatively associated with lacto-N-difucohexaose.
33677972	1	9	theme	HMOs	246:249	arg1	profiles					251:258	HMOs profiles	246:258	HMOs profiles	246:258	Human milk oligosaccharides (HMOs) play key roles in shaping infant fecal microbiota, and HMOs profiles have been reported to vary according to the mother's glycosyltransferase phenotype.
33677972	2	10	theme	time-of-flight	515:528	arg1	spectrometry					542:553	liquid chromatography quadrupole time-of-flight tandem mass spectrometry	482:553	liquid chromatography quadrupole time-of-flight tandem mass spectrometry	482:553	In this study, the profiles of HMOs in human milk from secretor or non-secretor mothers collected at 2 months postpartum were analyzed by liquid chromatography quadrupole time-of-flight tandem mass spectrometry.
33677972	6	11	theme	species-specific	1311:1326	arg1	manner					1328:1333	species-specific manner	1311:1333	species-specific manner	1311:1333	The present study provides the HMO profiles from Chinese mothers and their associations with infant fecal microbiota composition, suggesting that HMO compositions are associated with different Bifidobacterium strains in species-specific manner.
33677972	4	12	from	enriched	776:783	arg1	infants					788:794	infants	788:794	infants fed by non-secretor mothers	788:822	The amount of total and fucosylated HMOs were higher in secretor than non-secretor mothers, while Bifidobacterium genus were highly enriched in infants fed by non-secretor mothers.
33677972	6	13	theme	Bifidobacterium	1284:1298	arg1	strains					1300:1306	different Bifidobacterium strains	1274:1306	different Bifidobacterium strains in species-specific manner	1274:1333	The present study provides the HMO profiles from Chinese mothers and their associations with infant fecal microbiota composition, suggesting that HMO compositions are associated with different Bifidobacterium strains in species-specific manner.
33677972	6	14	theme	HMO	1122:1124	arg1	profiles					1126:1133	the HMO profiles	1118:1133	the HMO profiles from Chinese mothers and their associations with infant fecal microbiota composition	1118:1218	The present study provides the HMO profiles from Chinese mothers and their associations with infant fecal microbiota composition, suggesting that HMO compositions are associated with different Bifidobacterium strains in species-specific manner.
33677972	2	15	theme	quadrupole	504:513	arg1	spectrometry					542:553	liquid chromatography quadrupole time-of-flight tandem mass spectrometry	482:553	liquid chromatography quadrupole time-of-flight tandem mass spectrometry	482:553	In this study, the profiles of HMOs in human milk from secretor or non-secretor mothers collected at 2 months postpartum were analyzed by liquid chromatography quadrupole time-of-flight tandem mass spectrometry.
33677972	4	16	gly	fucosylated	668:678	arg1	HMOs					680:683	total and fucosylated HMOs	658:683	total and fucosylated HMOs	658:683	The amount of total and fucosylated HMOs were higher in secretor than non-secretor mothers, while Bifidobacterium genus were highly enriched in infants fed by non-secretor mothers.
33677972	6	17	theme	different	1274:1282	arg1	strains					1300:1306	different Bifidobacterium strains	1274:1306	different Bifidobacterium strains in species-specific manner	1274:1333	The present study provides the HMO profiles from Chinese mothers and their associations with infant fecal microbiota composition, suggesting that HMO compositions are associated with different Bifidobacterium strains in species-specific manner.
33677972	4	18	theme	non-secretor	714:725	arg1	mothers					727:733	non-secretor mothers	714:733	non-secretor mothers	714:733	The amount of total and fucosylated HMOs were higher in secretor than non-secretor mothers, while Bifidobacterium genus were highly enriched in infants fed by non-secretor mothers.
33677972	2	19	theme	chromatography	489:502	arg1	spectrometry					542:553	liquid chromatography quadrupole time-of-flight tandem mass spectrometry	482:553	liquid chromatography quadrupole time-of-flight tandem mass spectrometry	482:553	In this study, the profiles of HMOs in human milk from secretor or non-secretor mothers collected at 2 months postpartum were analyzed by liquid chromatography quadrupole time-of-flight tandem mass spectrometry.
33677972	6	20	with	associations	1166:1177	arg1	composition					1208:1218	infant fecal microbiota composition	1184:1218	infant fecal microbiota composition	1184:1218	The present study provides the HMO profiles from Chinese mothers and their associations with infant fecal microbiota composition, suggesting that HMO compositions are associated with different Bifidobacterium strains in species-specific manner.
33677972	2	21	theme	HMOs	375:378	arg1	profiles					363:370	the profiles	359:370	the profiles of HMOs in human milk from secretor or non-secretor mothers collected at 2 months postpartum	359:463	In this study, the profiles of HMOs in human milk from secretor or non-secretor mothers collected at 2 months postpartum were analyzed by liquid chromatography quadrupole time-of-flight tandem mass spectrometry.
33677972	0	22	theme	Fecal	105:109	arg1	Composition					122:132	the Fecal Microbiota Composition	101:132	the Fecal Microbiota Composition of Breastfed Infants	101:153	Maternal Fucosyltransferase 2 Status Associates with the Profiles of Human Milk Oligosaccharides and the Fecal Microbiota Composition of Breastfed Infants.
33677972	2	23	theme	non-secretor	411:422	arg1	mothers					424:430	secretor or non-secretor mothers	399:430	secretor or non-secretor mothers collected at 2 months postpartum	399:463	In this study, the profiles of HMOs in human milk from secretor or non-secretor mothers collected at 2 months postpartum were analyzed by liquid chromatography quadrupole time-of-flight tandem mass spectrometry.
33677972	0	24	theme	Microbiota	111:120	arg1	Composition					122:132	the Fecal Microbiota Composition	101:132	the Fecal Microbiota Composition of Breastfed Infants	101:153	Maternal Fucosyltransferase 2 Status Associates with the Profiles of Human Milk Oligosaccharides and the Fecal Microbiota Composition of Breastfed Infants.
33677972	0	25	theme	Fucosyltransferase	9:26	arg1	Status					30:35	Maternal Fucosyltransferase 2 Status	0:35	Maternal Fucosyltransferase 2 Status	0:35	Maternal Fucosyltransferase 2 Status Associates with the Profiles of Human Milk Oligosaccharides and the Fecal Microbiota Composition of Breastfed Infants.
33677972	4	26	theme	fucosylated	668:678	arg1	HMOs					680:683	total and fucosylated HMOs	658:683	total and fucosylated HMOs	658:683	The amount of total and fucosylated HMOs were higher in secretor than non-secretor mothers, while Bifidobacterium genus were highly enriched in infants fed by non-secretor mothers.
33677972	0	27	theme	Maternal	0:7	arg1	Status					30:35	Maternal Fucosyltransferase 2 Status	0:35	Maternal Fucosyltransferase 2 Status	0:35	Maternal Fucosyltransferase 2 Status Associates with the Profiles of Human Milk Oligosaccharides and the Fecal Microbiota Composition of Breastfed Infants.
33677972	6	28	theme	Chinese	1140:1146	arg1	mothers					1148:1154	Chinese mothers	1140:1154	Chinese mothers	1140:1154	The present study provides the HMO profiles from Chinese mothers and their associations with infant fecal microbiota composition, suggesting that HMO compositions are associated with different Bifidobacterium strains in species-specific manner.
33677972	2	29	theme	mass	537:540	arg1	spectrometry					542:553	liquid chromatography quadrupole time-of-flight tandem mass spectrometry	482:553	liquid chromatography quadrupole time-of-flight tandem mass spectrometry	482:553	In this study, the profiles of HMOs in human milk from secretor or non-secretor mothers collected at 2 months postpartum were analyzed by liquid chromatography quadrupole time-of-flight tandem mass spectrometry.
33677972	4	30	theme	non-secretor	803:814	arg1	mothers					816:822	non-secretor mothers	803:822	non-secretor mothers	803:822	The amount of total and fucosylated HMOs were higher in secretor than non-secretor mothers, while Bifidobacterium genus were highly enriched in infants fed by non-secretor mothers.
33677972	3	31	theme	16S	556:558	arg1	sequencing					565:574	16S rRNA sequencing	556:574	16S rRNA sequencing	556:574	16S rRNA sequencing was used to characterize the fecal microbiota of breastfed infants.
33677972	6	32	from	mothers	1148:1154	arg1	profiles					1126:1133	the HMO profiles	1118:1133	the HMO profiles from Chinese mothers and their associations with infant fecal microbiota composition	1118:1218	The present study provides the HMO profiles from Chinese mothers and their associations with infant fecal microbiota composition, suggesting that HMO compositions are associated with different Bifidobacterium strains in species-specific manner.
33677972	1	33	theme	key	196:198	arg1	roles					200:204	key roles	196:204	key roles	196:204	Human milk oligosaccharides (HMOs) play key roles in shaping infant fecal microbiota, and HMOs profiles have been reported to vary according to the mother's glycosyltransferase phenotype.
33677972	2	34	theme	secretor	399:406	arg1	mothers					424:430	secretor or non-secretor mothers	399:430	secretor or non-secretor mothers collected at 2 months postpartum	399:463	In this study, the profiles of HMOs in human milk from secretor or non-secretor mothers collected at 2 months postpartum were analyzed by liquid chromatography quadrupole time-of-flight tandem mass spectrometry.
33677972	6	35	with	mothers	1148:1154	arg1	composition					1208:1218	infant fecal microbiota composition	1184:1218	infant fecal microbiota composition	1184:1218	The present study provides the HMO profiles from Chinese mothers and their associations with infant fecal microbiota composition, suggesting that HMO compositions are associated with different Bifidobacterium strains in species-specific manner.
33677972	3	36	theme	rRNA	560:563	arg1	sequencing					565:574	16S rRNA sequencing	556:574	16S rRNA sequencing	556:574	16S rRNA sequencing was used to characterize the fecal microbiota of breastfed infants.
33677972	6	37	theme	fecal	1191:1195	arg1	composition					1208:1218	infant fecal microbiota composition	1184:1218	infant fecal microbiota composition	1184:1218	The present study provides the HMO profiles from Chinese mothers and their associations with infant fecal microbiota composition, suggesting that HMO compositions are associated with different Bifidobacterium strains in species-specific manner.
33677972	2	38	theme	months	447:452	arg1	postpartum					454:463	2 months postpartum	445:463	2 months postpartum	445:463	In this study, the profiles of HMOs in human milk from secretor or non-secretor mothers collected at 2 months postpartum were analyzed by liquid chromatography quadrupole time-of-flight tandem mass spectrometry.
33677972	0	39	theme	Infants	147:153	arg1	Composition					122:132	the Fecal Microbiota Composition	101:132	the Fecal Microbiota Composition of Breastfed Infants	101:153	Maternal Fucosyltransferase 2 Status Associates with the Profiles of Human Milk Oligosaccharides and the Fecal Microbiota Composition of Breastfed Infants.
33677972	0	39	theme	Infants	147:153	arg1	Profiles					57:64	the Profiles	53:64	the Profiles of Human Milk Oligosaccharides	53:95	Maternal Fucosyltransferase 2 Status Associates with the Profiles of Human Milk Oligosaccharides and the Fecal Microbiota Composition of Breastfed Infants.
33677972	5	40	theme	infant	855:860	arg1	microbiota					868:877	infant fecal microbiota	855:877	infant fecal microbiota	855:877	Associations between HMOs and infant fecal microbiota showed that the relative abundance of Bifidobacterium-OTU158 was positively associated with 2'-fucosyllactose and 3-fucosyllactose, and Bifidobacterium-OTU90 was negatively associated with lacto-N-difucohexaose.
33677972	2	41	theme	human	383:387	arg1	milk					389:392	human milk	383:392	human milk from secretor or non-secretor mothers collected at 2 months postpartum	383:463	In this study, the profiles of HMOs in human milk from secretor or non-secretor mothers collected at 2 months postpartum were analyzed by liquid chromatography quadrupole time-of-flight tandem mass spectrometry.
33677972	4	42	theme	total	658:662	arg1	HMOs					680:683	total and fucosylated HMOs	658:683	total and fucosylated HMOs	658:683	The amount of total and fucosylated HMOs were higher in secretor than non-secretor mothers, while Bifidobacterium genus were highly enriched in infants fed by non-secretor mothers.
33677972	0	43	theme	Breastfed	137:145	arg1	Infants					147:153	Breastfed Infants	137:153	Breastfed Infants	137:153	Maternal Fucosyltransferase 2 Status Associates with the Profiles of Human Milk Oligosaccharides and the Fecal Microbiota Composition of Breastfed Infants.
33677972	3	44	theme	infants	635:641	arg1	microbiota					611:620	the fecal microbiota	601:620	the fecal microbiota of breastfed infants	601:641	16S rRNA sequencing was used to characterize the fecal microbiota of breastfed infants.
33677972	2	45	from	profiles	363:370	arg1	mothers					424:430	secretor or non-secretor mothers	399:430	secretor or non-secretor mothers collected at 2 months postpartum	399:463	In this study, the profiles of HMOs in human milk from secretor or non-secretor mothers collected at 2 months postpartum were analyzed by liquid chromatography quadrupole time-of-flight tandem mass spectrometry.
33677972	2	45	from	profiles	363:370	arg1	milk					389:392	human milk	383:392	human milk from secretor or non-secretor mothers collected at 2 months postpartum	383:463	In this study, the profiles of HMOs in human milk from secretor or non-secretor mothers collected at 2 months postpartum were analyzed by liquid chromatography quadrupole time-of-flight tandem mass spectrometry.
33677972	1	46	theme	shaping	209:215	arg1	microbiota					230:239	shaping infant fecal microbiota	209:239	shaping infant fecal microbiota	209:239	Human milk oligosaccharides (HMOs) play key roles in shaping infant fecal microbiota, and HMOs profiles have been reported to vary according to the mother's glycosyltransferase phenotype.
33677972	2	47	theme	tandem	530:535	arg1	spectrometry					542:553	liquid chromatography quadrupole time-of-flight tandem mass spectrometry	482:553	liquid chromatography quadrupole time-of-flight tandem mass spectrometry	482:553	In this study, the profiles of HMOs in human milk from secretor or non-secretor mothers collected at 2 months postpartum were analyzed by liquid chromatography quadrupole time-of-flight tandem mass spectrometry.
33677972	6	48	theme	HMO	1237:1239	arg1	compositions					1241:1252	HMO compositions	1237:1252	HMO compositions	1237:1252	The present study provides the HMO profiles from Chinese mothers and their associations with infant fecal microbiota composition, suggesting that HMO compositions are associated with different Bifidobacterium strains in species-specific manner.
33677972	3	49	used	used	580:583	arg2	sequencing					565:574	16S rRNA sequencing	556:574	16S rRNA sequencing	556:574	16S rRNA sequencing was used to characterize the fecal microbiota of breastfed infants.
33677972	2	50	theme	liquid	482:487	arg1	spectrometry					542:553	liquid chromatography quadrupole time-of-flight tandem mass spectrometry	482:553	liquid chromatography quadrupole time-of-flight tandem mass spectrometry	482:553	In this study, the profiles of HMOs in human milk from secretor or non-secretor mothers collected at 2 months postpartum were analyzed by liquid chromatography quadrupole time-of-flight tandem mass spectrometry.
33677972	1	51	theme	infant	217:222	arg1	microbiota					230:239	shaping infant fecal microbiota	209:239	shaping infant fecal microbiota	209:239	Human milk oligosaccharides (HMOs) play key roles in shaping infant fecal microbiota, and HMOs profiles have been reported to vary according to the mother's glycosyltransferase phenotype.
33677972	3	52	theme	breastfed	625:633	arg1	infants					635:641	breastfed infants	625:641	breastfed infants	625:641	16S rRNA sequencing was used to characterize the fecal microbiota of breastfed infants.
33677972	4	53	theme	HMOs	680:683	arg1	higher					690:695	higher	690:695	higher	690:695	The amount of total and fucosylated HMOs were higher in secretor than non-secretor mothers, while Bifidobacterium genus were highly enriched in infants fed by non-secretor mothers.
33677972	4	53	theme	HMOs	680:683	arg1	HMOs					680:683	total and fucosylated HMOs	658:683	total and fucosylated HMOs	658:683	The amount of total and fucosylated HMOs were higher in secretor than non-secretor mothers, while Bifidobacterium genus were highly enriched in infants fed by non-secretor mothers.
33677972	4	53	theme	HMOs	680:683	arg1	amount					648:653	The amount	644:653	The amount of total and fucosylated HMOs	644:683	The amount of total and fucosylated HMOs were higher in secretor than non-secretor mothers, while Bifidobacterium genus were highly enriched in infants fed by non-secretor mothers.
33677972	1	54	theme	fecal	224:228	arg1	microbiota					230:239	shaping infant fecal microbiota	209:239	shaping infant fecal microbiota	209:239	Human milk oligosaccharides (HMOs) play key roles in shaping infant fecal microbiota, and HMOs profiles have been reported to vary according to the mother's glycosyltransferase phenotype.
33677972	0	55	theme	Human	69:73	arg1	Oligosaccharides					80:95	Human Milk Oligosaccharides	69:95	Human Milk Oligosaccharides	69:95	Maternal Fucosyltransferase 2 Status Associates with the Profiles of Human Milk Oligosaccharides and the Fecal Microbiota Composition of Breastfed Infants.
33677972	5	56	theme	fecal	862:866	arg1	microbiota					868:877	infant fecal microbiota	855:877	infant fecal microbiota	855:877	Associations between HMOs and infant fecal microbiota showed that the relative abundance of Bifidobacterium-OTU158 was positively associated with 2'-fucosyllactose and 3-fucosyllactose, and Bifidobacterium-OTU90 was negatively associated with lacto-N-difucohexaose.
33677972	3	57	theme	fecal	605:609	arg1	microbiota					611:620	the fecal microbiota	601:620	the fecal microbiota of breastfed infants	601:641	16S rRNA sequencing was used to characterize the fecal microbiota of breastfed infants.
33677972	6	58	from	associations	1166:1177	arg1	profiles					1126:1133	the HMO profiles	1118:1133	the HMO profiles from Chinese mothers and their associations with infant fecal microbiota composition	1118:1218	The present study provides the HMO profiles from Chinese mothers and their associations with infant fecal microbiota composition, suggesting that HMO compositions are associated with different Bifidobacterium strains in species-specific manner.
33677972	5	59	theme	relative	895:902	arg1	abundance					904:912	the relative abundance	891:912	the relative abundance of Bifidobacterium-OTU158	891:938	Associations between HMOs and infant fecal microbiota showed that the relative abundance of Bifidobacterium-OTU158 was positively associated with 2'-fucosyllactose and 3-fucosyllactose, and Bifidobacterium-OTU90 was negatively associated with lacto-N-difucohexaose.
33677972	6	60	theme	present	1095:1101	arg1	study					1103:1107	The present study	1091:1107	The present study	1091:1107	The present study provides the HMO profiles from Chinese mothers and their associations with infant fecal microbiota composition, suggesting that HMO compositions are associated with different Bifidobacterium strains in species-specific manner.
33677972	6	61	theme	microbiota	1197:1206	arg1	composition					1208:1218	infant fecal microbiota composition	1184:1218	infant fecal microbiota composition	1184:1218	The present study provides the HMO profiles from Chinese mothers and their associations with infant fecal microbiota composition, suggesting that HMO compositions are associated with different Bifidobacterium strains in species-specific manner.
33677972	1	62	theme	Human	156:160	arg1	HMOs					185:188	HMOs	185:188	HMOs	185:188	Human milk oligosaccharides (HMOs) play key roles in shaping infant fecal microbiota, and HMOs profiles have been reported to vary according to the mother's glycosyltransferase phenotype.
33677972	1	62	theme	Human	156:160	arg1	oligosaccharides					167:182	Human milk oligosaccharides	156:182	Human milk oligosaccharides (HMOs)	156:189	Human milk oligosaccharides (HMOs) play key roles in shaping infant fecal microbiota, and HMOs profiles have been reported to vary according to the mother's glycosyltransferase phenotype.
32723024	8	0	theme	cell	1233:1236	arg1	viability					1238:1246	higher cell viability	1226:1246	higher cell viability	1226:1246	When cultured with porcine valve interstitial cells (VICs), the fiber-reinforced composites were able to maintain higher cell viability compared with fiber-only samples.
32723024	4	1	theme	interconnected	634:647	arg1	structures					656:665	interconnected porous structures	634:665	interconnected porous structures	634:665	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC possessed interconnected porous structures and porosity higher than fiber-only conditions.
32723024	7	2	theme	steady-state	1070:1081	arg1	release					1083:1089	a steady-state release	1068:1089	a steady-state release after the first day	1068:1109	Elution studies revealed that less than 20% of incorporated gelatin methacrylate and GAGs were released over 2 weeks, with a steady-state release after the first day.
32723024	8	3	theme	higher	1226:1231	arg1	viability					1238:1246	higher cell viability	1226:1246	higher cell viability	1226:1246	When cultured with porcine valve interstitial cells (VICs), the fiber-reinforced composites were able to maintain higher cell viability compared with fiber-only samples.
32723024	4	4	theme	gelatin	583:589	arg1	GAG					610:612	GAG	610:612	GAG	610:612	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC possessed interconnected porous structures and porosity higher than fiber-only conditions.
32723024	4	4	theme	gelatin	583:589	arg1	glycosaminoglycan					591:607	fiber-reinforced gelatin glycosaminoglycan	566:607	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC	562:622	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC possessed interconnected porous structures and porosity higher than fiber-only conditions.
32723024	5	5	theme	compressive	752:762	arg1	modulus					764:770	compressive modulus	752:770	compressive modulus	752:770	These fiber-reinforced HCs exhibited compressive modulus and biaxial mechanical behavior comparable to that of native porcine aortic valves.
32723024	4	6	theme	porous	649:654	arg1	structures					656:665	interconnected porous structures	634:665	interconnected porous structures	634:665	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC possessed interconnected porous structures and porosity higher than fiber-only conditions.
32723024	5	7	theme	porcine	833:839	arg1	valves					848:853	native porcine aortic valves	826:853	native porcine aortic valves	826:853	These fiber-reinforced HCs exhibited compressive modulus and biaxial mechanical behavior comparable to that of native porcine aortic valves.
32723024	4	8	theme	fiber-reinforced	566:581	arg1	GAG					610:612	GAG	610:612	GAG	610:612	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC possessed interconnected porous structures and porosity higher than fiber-only conditions.
32723024	4	8	theme	fiber-reinforced	566:581	arg1	glycosaminoglycan					591:607	fiber-reinforced gelatin glycosaminoglycan	566:607	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC	562:622	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC possessed interconnected porous structures and porosity higher than fiber-only conditions.
32723024	8	9	theme	porcine	1131:1137	arg1	VICs					1165:1168	VICs	1165:1168	VICs	1165:1168	When cultured with porcine valve interstitial cells (VICs), the fiber-reinforced composites were able to maintain higher cell viability compared with fiber-only samples.
32723024	8	9	theme	porcine	1131:1137	arg1	cells					1158:1162	porcine valve interstitial cells	1131:1162	porcine valve interstitial cells (VICs)	1131:1169	When cultured with porcine valve interstitial cells (VICs), the fiber-reinforced composites were able to maintain higher cell viability compared with fiber-only samples.
32723024	2	10	contain	possessed	319:327	arg2	diameters					335:343	fiber diameters	329:343	fiber diameters	329:343	Polycaprolactone and gelatin (3:1) blends were jet-spun to fabricate aligned fibers that possessed fiber diameters in the range found in the native heart valve.
32723024	2	10	contain	possessed	319:327	arg1	fibers					307:312	aligned fibers	299:312	aligned fibers that possessed fiber diameters in the range found in the native heart valve	299:388	Polycaprolactone and gelatin (3:1) blends were jet-spun to fabricate aligned fibers that possessed fiber diameters in the range found in the native heart valve.
32723024	4	11	contain	possessed	624:632	arg1	HC					621:622	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC	562:622	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC	562:622	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC possessed interconnected porous structures and porosity higher than fiber-only conditions.
32723024	4	11	contain	possessed	624:632	arg2	porosity					671:678	porosity	671:678	porosity higher than fiber-only conditions	671:712	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC possessed interconnected porous structures and porosity higher than fiber-only conditions.
32723024	4	11	contain	possessed	624:632	arg2	structures					656:665	interconnected porous structures	634:665	interconnected porous structures	634:665	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC possessed interconnected porous structures and porosity higher than fiber-only conditions.
32723024	9	12	theme	Quiescent	1282:1290	arg1	VICs					1292:1295	Quiescent VICs	1282:1295	Quiescent VICs	1282:1295	Quiescent VICs expressed alpha smooth muscle actin and calponin showing an activated phenotype, along with a few cells expressing the proliferation marker Ki67 and negative expression for RUNX2, an osteogenic marker.
32723024	2	13	theme	heart	378:382	arg1	valve					384:388	the native heart valve	367:388	the native heart valve	367:388	Polycaprolactone and gelatin (3:1) blends were jet-spun to fabricate aligned fibers that possessed fiber diameters in the range found in the native heart valve.
32723024	8	14	theme	fiber-only	1262:1271	arg1	samples					1273:1279	fiber-only samples	1262:1279	fiber-only samples	1262:1279	When cultured with porcine valve interstitial cells (VICs), the fiber-reinforced composites were able to maintain higher cell viability compared with fiber-only samples.
32723024	3	15	from	hydrogels	435:443	arg1	embedded					409:416	embedded	409:416	embedded	409:416	These fibers were embedded in methacrylated hydrogels made from gelatin, sodium hyaluronate, and chondroitin sulfate to create fiber-reinforced hydrogel composites (HCs).
32723024	8	16	theme	interstitial	1145:1156	arg1	VICs					1165:1168	VICs	1165:1168	VICs	1165:1168	When cultured with porcine valve interstitial cells (VICs), the fiber-reinforced composites were able to maintain higher cell viability compared with fiber-only samples.
32723024	8	16	theme	interstitial	1145:1156	arg1	cells					1158:1162	porcine valve interstitial cells	1131:1162	porcine valve interstitial cells (VICs)	1131:1169	When cultured with porcine valve interstitial cells (VICs), the fiber-reinforced composites were able to maintain higher cell viability compared with fiber-only samples.
32723024	10	17	theme	high	1702:1705	arg1	viability					1712:1720	high cell viability	1702:1720	high cell viability	1702:1720	Our study demonstrated that compared with the hydrogels and fibers alone, combining both components can yield durable, reinforced composites that mimic heart valve mechanical behavior, while maintaining high cell viability and expressing positive activation as well as proliferation markers.
32723024	3	18	theme	chondroitin	488:498	arg1	sulfate					500:506	chondroitin sulfate	488:506	chondroitin sulfate	488:506	These fibers were embedded in methacrylated hydrogels made from gelatin, sodium hyaluronate, and chondroitin sulfate to create fiber-reinforced hydrogel composites (HCs).
32723024	10	19	theme	valve	1657:1661	arg1	behavior					1674:1681	heart valve mechanical behavior	1651:1681	heart valve mechanical behavior	1651:1681	Our study demonstrated that compared with the hydrogels and fibers alone, combining both components can yield durable, reinforced composites that mimic heart valve mechanical behavior, while maintaining high cell viability and expressing positive activation as well as proliferation markers.
32723024	8	20	theme	valve	1139:1143	arg1	VICs					1165:1168	VICs	1165:1168	VICs	1165:1168	When cultured with porcine valve interstitial cells (VICs), the fiber-reinforced composites were able to maintain higher cell viability compared with fiber-only samples.
32723024	8	20	theme	valve	1139:1143	arg1	cells					1158:1162	porcine valve interstitial cells	1131:1162	porcine valve interstitial cells (VICs)	1131:1169	When cultured with porcine valve interstitial cells (VICs), the fiber-reinforced composites were able to maintain higher cell viability compared with fiber-only samples.
32723024	9	21	theme	osteogenic	1480:1489	arg1	marker					1491:1496	an osteogenic marker	1477:1496	an osteogenic marker	1477:1496	Quiescent VICs expressed alpha smooth muscle actin and calponin showing an activated phenotype, along with a few cells expressing the proliferation marker Ki67 and negative expression for RUNX2, an osteogenic marker.
32723024	9	21	theme	osteogenic	1480:1489	arg1	RUNX2					1470:1474	RUNX2	1470:1474	RUNX2	1470:1474	Quiescent VICs expressed alpha smooth muscle actin and calponin showing an activated phenotype, along with a few cells expressing the proliferation marker Ki67 and negative expression for RUNX2, an osteogenic marker.
32723024	10	22	theme	mechanical	1663:1672	arg1	behavior					1674:1681	heart valve mechanical behavior	1651:1681	heart valve mechanical behavior	1651:1681	Our study demonstrated that compared with the hydrogels and fibers alone, combining both components can yield durable, reinforced composites that mimic heart valve mechanical behavior, while maintaining high cell viability and expressing positive activation as well as proliferation markers.
32723024	0	23	theme	Fiber-Reinforced	12:27	arg1	Hydrogels					47:55	Anisotropic Fiber-Reinforced Glycosaminoglycan Hydrogels	0:55	Anisotropic Fiber-Reinforced Glycosaminoglycan Hydrogels for Heart	0:65	Anisotropic Fiber-Reinforced Glycosaminoglycan Hydrogels for Heart Valve Tissue Engineering.
32723024	9	24	theme	proliferation	1416:1428	arg1	Ki67					1437:1440	proliferation marker Ki67	1416:1440	proliferation marker Ki67	1416:1440	Quiescent VICs expressed alpha smooth muscle actin and calponin showing an activated phenotype, along with a few cells expressing the proliferation marker Ki67 and negative expression for RUNX2, an osteogenic marker.
32723024	5	25	theme	fiber-reinforced	721:736	arg1	HCs					738:740	These fiber-reinforced HCs	715:740	These fiber-reinforced HCs	715:740	These fiber-reinforced HCs exhibited compressive modulus and biaxial mechanical behavior comparable to that of native porcine aortic valves.
32723024	9	26	theme	alpha	1307:1311	arg1	actin					1327:1331	alpha smooth muscle actin	1307:1331	alpha smooth muscle actin	1307:1331	Quiescent VICs expressed alpha smooth muscle actin and calponin showing an activated phenotype, along with a few cells expressing the proliferation marker Ki67 and negative expression for RUNX2, an osteogenic marker.
32723024	2	27	theme	native	371:376	arg1	valve					384:388	the native heart valve	367:388	the native heart valve	367:388	Polycaprolactone and gelatin (3:1) blends were jet-spun to fabricate aligned fibers that possessed fiber diameters in the range found in the native heart valve.
32723024	8	28	theme	fiber-reinforced	1176:1191	arg1	composites					1193:1202	the fiber-reinforced composites	1172:1202	the fiber-reinforced composites	1172:1202	When cultured with porcine valve interstitial cells (VICs), the fiber-reinforced composites were able to maintain higher cell viability compared with fiber-only samples.
32723024	8	28	theme	fiber-reinforced	1176:1191	arg1	able					1209:1212	able	1209:1212	able	1209:1212	When cultured with porcine valve interstitial cells (VICs), the fiber-reinforced composites were able to maintain higher cell viability compared with fiber-only samples.
32723024	0	29	theme	Anisotropic	0:10	arg1	Hydrogels					47:55	Anisotropic Fiber-Reinforced Glycosaminoglycan Hydrogels	0:55	Anisotropic Fiber-Reinforced Glycosaminoglycan Hydrogels for Heart	0:65	Anisotropic Fiber-Reinforced Glycosaminoglycan Hydrogels for Heart Valve Tissue Engineering.
32723024	9	30	theme	marker	1430:1435	arg1	Ki67					1437:1440	proliferation marker Ki67	1416:1440	proliferation marker Ki67	1416:1440	Quiescent VICs expressed alpha smooth muscle actin and calponin showing an activated phenotype, along with a few cells expressing the proliferation marker Ki67 and negative expression for RUNX2, an osteogenic marker.
32723024	7	31	theme	GAGs	1030:1033	arg1	%					987:987	less than 20%	975:987	less than 20% of incorporated gelatin methacrylate and GAGs	975:1033	Elution studies revealed that less than 20% of incorporated gelatin methacrylate and GAGs were released over 2 weeks, with a steady-state release after the first day.
32723024	7	31	theme	GAGs	1030:1033	arg1	GAGs					1030:1033	GAGs	1030:1033	GAGs	1030:1033	Elution studies revealed that less than 20% of incorporated gelatin methacrylate and GAGs were released over 2 weeks, with a steady-state release after the first day.
32723024	7	31	theme	GAGs	1030:1033	arg1	methacrylate					1013:1024	incorporated gelatin methacrylate	992:1024	incorporated gelatin methacrylate	992:1024	Elution studies revealed that less than 20% of incorporated gelatin methacrylate and GAGs were released over 2 weeks, with a steady-state release after the first day.
32723024	1	32	theme	polymer	117:123	arg1	hydrogels					171:179	polymer fiber-reinforced protein-polysaccharide-based hydrogels	117:179	polymer fiber-reinforced protein-polysaccharide-based hydrogels for heart valve tissue engineering applications	117:227	This study investigates polymer fiber-reinforced protein-polysaccharide-based hydrogels for heart valve tissue engineering applications.
32723024	3	33	theme	methacrylated	421:433	arg1	hydrogels					435:443	methacrylated hydrogels	421:443	methacrylated hydrogels made from gelatin, sodium hyaluronate, and chondroitin sulfate to create fiber-reinforced hydrogel composites (HCs)	421:559	These fibers were embedded in methacrylated hydrogels made from gelatin, sodium hyaluronate, and chondroitin sulfate to create fiber-reinforced hydrogel composites (HCs).
32723024	4	34	theme	-based	614:619	arg1	HC					621:622	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC	562:622	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC	562:622	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC possessed interconnected porous structures and porosity higher than fiber-only conditions.
32723024	9	35	theme	muscle	1320:1325	arg1	actin					1327:1331	alpha smooth muscle actin	1307:1331	alpha smooth muscle actin	1307:1331	Quiescent VICs expressed alpha smooth muscle actin and calponin showing an activated phenotype, along with a few cells expressing the proliferation marker Ki67 and negative expression for RUNX2, an osteogenic marker.
32723024	1	36	theme	fiber-reinforced	125:140	arg1	hydrogels					171:179	polymer fiber-reinforced protein-polysaccharide-based hydrogels	117:179	polymer fiber-reinforced protein-polysaccharide-based hydrogels for heart valve tissue engineering applications	117:227	This study investigates polymer fiber-reinforced protein-polysaccharide-based hydrogels for heart valve tissue engineering applications.
32723024	7	37	theme	Elution	945:951	arg1	studies					953:959	Elution studies	945:959	Elution studies	945:959	Elution studies revealed that less than 20% of incorporated gelatin methacrylate and GAGs were released over 2 weeks, with a steady-state release after the first day.
32723024	3	38	theme	fiber-reinforced	518:533	arg1	HCs					556:558	HCs	556:558	HCs	556:558	These fibers were embedded in methacrylated hydrogels made from gelatin, sodium hyaluronate, and chondroitin sulfate to create fiber-reinforced hydrogel composites (HCs).
32723024	3	38	theme	fiber-reinforced	518:533	arg1	composites					544:553	fiber-reinforced hydrogel composites	518:553	fiber-reinforced hydrogel composites (HCs)	518:559	These fibers were embedded in methacrylated hydrogels made from gelatin, sodium hyaluronate, and chondroitin sulfate to create fiber-reinforced hydrogel composites (HCs).
32723024	0	39	theme	Glycosaminoglycan	29:45	arg1	Hydrogels					47:55	Anisotropic Fiber-Reinforced Glycosaminoglycan Hydrogels	0:55	Anisotropic Fiber-Reinforced Glycosaminoglycan Hydrogels for Heart	0:65	Anisotropic Fiber-Reinforced Glycosaminoglycan Hydrogels for Heart Valve Tissue Engineering.
32723024	2	40	located	found	358:362	arg2	range					352:356	the range	348:356	the range found in the native heart valve	348:388	Polycaprolactone and gelatin (3:1) blends were jet-spun to fabricate aligned fibers that possessed fiber diameters in the range found in the native heart valve.
32723024	2	40	located	found	358:362	arg1	valve					384:388	the native heart valve	367:388	the native heart valve	367:388	Polycaprolactone and gelatin (3:1) blends were jet-spun to fabricate aligned fibers that possessed fiber diameters in the range found in the native heart valve.
32723024	10	41	theme	positive	1737:1744	arg1	activation					1746:1755	positive activation	1737:1755	positive activation	1737:1755	Our study demonstrated that compared with the hydrogels and fibers alone, combining both components can yield durable, reinforced composites that mimic heart valve mechanical behavior, while maintaining high cell viability and expressing positive activation as well as proliferation markers.
32723024	1	42	theme	protein-polysaccharide-based	142:169	arg1	hydrogels					171:179	polymer fiber-reinforced protein-polysaccharide-based hydrogels	117:179	polymer fiber-reinforced protein-polysaccharide-based hydrogels for heart valve tissue engineering applications	117:227	This study investigates polymer fiber-reinforced protein-polysaccharide-based hydrogels for heart valve tissue engineering applications.
32723024	3	43	theme	hydrogel	535:542	arg1	HCs					556:558	HCs	556:558	HCs	556:558	These fibers were embedded in methacrylated hydrogels made from gelatin, sodium hyaluronate, and chondroitin sulfate to create fiber-reinforced hydrogel composites (HCs).
32723024	3	43	theme	hydrogel	535:542	arg1	composites					544:553	fiber-reinforced hydrogel composites	518:553	fiber-reinforced hydrogel composites (HCs)	518:559	These fibers were embedded in methacrylated hydrogels made from gelatin, sodium hyaluronate, and chondroitin sulfate to create fiber-reinforced hydrogel composites (HCs).
32723024	3	44	theme	sodium	464:469	arg1	hyaluronate					471:481	sodium hyaluronate	464:481	sodium hyaluronate	464:481	These fibers were embedded in methacrylated hydrogels made from gelatin, sodium hyaluronate, and chondroitin sulfate to create fiber-reinforced hydrogel composites (HCs).
32723024	9	45	theme	negative	1446:1453	arg1	expression					1455:1464	negative expression	1446:1464	negative expression	1446:1464	Quiescent VICs expressed alpha smooth muscle actin and calponin showing an activated phenotype, along with a few cells expressing the proliferation marker Ki67 and negative expression for RUNX2, an osteogenic marker.
32723024	2	46	theme	gelatin	251:257	arg1	blends					265:270	Polycaprolactone and gelatin (3:1) blends	230:270	Polycaprolactone and gelatin (3:1) blends	230:270	Polycaprolactone and gelatin (3:1) blends were jet-spun to fabricate aligned fibers that possessed fiber diameters in the range found in the native heart valve.
32723024	2	47	theme	aligned	299:305	arg1	fibers					307:312	aligned fibers	299:312	aligned fibers that possessed fiber diameters in the range found in the native heart valve	299:388	Polycaprolactone and gelatin (3:1) blends were jet-spun to fabricate aligned fibers that possessed fiber diameters in the range found in the native heart valve.
32723024	5	48	theme	native	826:831	arg1	valves					848:853	native porcine aortic valves	826:853	native porcine aortic valves	826:853	These fiber-reinforced HCs exhibited compressive modulus and biaxial mechanical behavior comparable to that of native porcine aortic valves.
32723024	3	49	from	embedded	409:416	arg1	hydrogels					435:443	methacrylated hydrogels	421:443	methacrylated hydrogels made from gelatin, sodium hyaluronate, and chondroitin sulfate to create fiber-reinforced hydrogel composites (HCs)	421:559	These fibers were embedded in methacrylated hydrogels made from gelatin, sodium hyaluronate, and chondroitin sulfate to create fiber-reinforced hydrogel composites (HCs).
32723024	10	50	theme	proliferation	1768:1780	arg1	markers					1782:1788	proliferation markers	1768:1788	both components can yield durable, reinforced composites that mimic heart valve mechanical behavior, while maintaining high cell viability and expressing positive activation as well as proliferation markers	1583:1788	Our study demonstrated that compared with the hydrogels and fibers alone, combining both components can yield durable, reinforced composites that mimic heart valve mechanical behavior, while maintaining high cell viability and expressing positive activation as well as proliferation markers.
32723024	0	51	theme	Tissue	73:78	arg1	Engineering					80:90	Tissue Engineering	73:90	Tissue Engineering	73:90	Anisotropic Fiber-Reinforced Glycosaminoglycan Hydrogels for Heart Valve Tissue Engineering.
32723024	4	52	theme	glycosaminoglycan	591:607	arg1	HC					621:622	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC	562:622	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC	562:622	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC possessed interconnected porous structures and porosity higher than fiber-only conditions.
32723024	5	53	theme	biaxial	776:782	arg1	behavior					795:802	biaxial mechanical behavior	776:802	biaxial mechanical behavior	776:802	These fiber-reinforced HCs exhibited compressive modulus and biaxial mechanical behavior comparable to that of native porcine aortic valves.
32723024	2	54	theme	Polycaprolactone	230:245	arg1	blends					265:270	Polycaprolactone and gelatin (3:1) blends	230:270	Polycaprolactone and gelatin (3:1) blends	230:270	Polycaprolactone and gelatin (3:1) blends were jet-spun to fabricate aligned fibers that possessed fiber diameters in the range found in the native heart valve.
32723024	9	55	theme	few	1391:1393	arg1	cells					1395:1399	a few cells	1389:1399	a few cells expressing the proliferation marker Ki67 and negative expression for RUNX2, an osteogenic marker	1389:1496	Quiescent VICs expressed alpha smooth muscle actin and calponin showing an activated phenotype, along with a few cells expressing the proliferation marker Ki67 and negative expression for RUNX2, an osteogenic marker.
32723024	9	56	theme	smooth	1313:1318	arg1	actin					1327:1331	alpha smooth muscle actin	1307:1331	alpha smooth muscle actin	1307:1331	Quiescent VICs expressed alpha smooth muscle actin and calponin showing an activated phenotype, along with a few cells expressing the proliferation marker Ki67 and negative expression for RUNX2, an osteogenic marker.
32723024	5	57	theme	mechanical	784:793	arg1	behavior					795:802	biaxial mechanical behavior	776:802	biaxial mechanical behavior	776:802	These fiber-reinforced HCs exhibited compressive modulus and biaxial mechanical behavior comparable to that of native porcine aortic valves.
32723024	6	58	theme	fiber-reinforced	860:875	arg1	able					886:889	able	886:889	able	886:889	The fiber-reinforced HCs were able to swell higher and degraded less than the hydrogels.
32723024	6	58	theme	fiber-reinforced	860:875	arg1	HCs					877:879	The fiber-reinforced HCs	856:879	The fiber-reinforced HCs	856:879	The fiber-reinforced HCs were able to swell higher and degraded less than the hydrogels.
32723024	7	59	theme	methacrylate	1013:1024	arg1	%					987:987	less than 20%	975:987	less than 20% of incorporated gelatin methacrylate and GAGs	975:1033	Elution studies revealed that less than 20% of incorporated gelatin methacrylate and GAGs were released over 2 weeks, with a steady-state release after the first day.
32723024	7	59	theme	methacrylate	1013:1024	arg1	GAGs					1030:1033	GAGs	1030:1033	GAGs	1030:1033	Elution studies revealed that less than 20% of incorporated gelatin methacrylate and GAGs were released over 2 weeks, with a steady-state release after the first day.
32723024	7	59	theme	methacrylate	1013:1024	arg1	methacrylate					1013:1024	incorporated gelatin methacrylate	992:1024	incorporated gelatin methacrylate	992:1024	Elution studies revealed that less than 20% of incorporated gelatin methacrylate and GAGs were released over 2 weeks, with a steady-state release after the first day.
32723024	10	60	theme	durable	1609:1615	arg1	composites					1629:1638	durable, reinforced composites	1609:1638	durable, reinforced composites that mimic heart valve mechanical behavior, while maintaining high cell viability and expressing positive activation	1609:1755	Our study demonstrated that compared with the hydrogels and fibers alone, combining both components can yield durable, reinforced composites that mimic heart valve mechanical behavior, while maintaining high cell viability and expressing positive activation as well as proliferation markers.
32723024	9	61	theme	activated	1357:1365	arg1	phenotype					1367:1375	an activated phenotype	1354:1375	an activated phenotype	1354:1375	Quiescent VICs expressed alpha smooth muscle actin and calponin showing an activated phenotype, along with a few cells expressing the proliferation marker Ki67 and negative expression for RUNX2, an osteogenic marker.
32723024	2	62	theme	fiber	329:333	arg1	diameters					335:343	fiber diameters	329:343	fiber diameters	329:343	Polycaprolactone and gelatin (3:1) blends were jet-spun to fabricate aligned fibers that possessed fiber diameters in the range found in the native heart valve.
32723024	7	63	theme	first	1101:1105	arg1	day					1107:1109	the first day	1097:1109	the first day	1097:1109	Elution studies revealed that less than 20% of incorporated gelatin methacrylate and GAGs were released over 2 weeks, with a steady-state release after the first day.
32723024	1	64	theme	heart	185:189	arg1	applications					216:227	heart valve tissue engineering applications	185:227	heart valve tissue engineering applications	185:227	This study investigates polymer fiber-reinforced protein-polysaccharide-based hydrogels for heart valve tissue engineering applications.
32723024	10	65	dep	durable	1609:1615	arg1	reinforced					1618:1627	reinforced	1618:1627	reinforced	1618:1627	Our study demonstrated that compared with the hydrogels and fibers alone, combining both components can yield durable, reinforced composites that mimic heart valve mechanical behavior, while maintaining high cell viability and expressing positive activation as well as proliferation markers.
32723024	5	66	theme	comparable	804:813	arg1	modulus					764:770	compressive modulus	752:770	compressive modulus	752:770	These fiber-reinforced HCs exhibited compressive modulus and biaxial mechanical behavior comparable to that of native porcine aortic valves.
32723024	1	67	theme	valve	191:195	arg1	applications					216:227	heart valve tissue engineering applications	185:227	heart valve tissue engineering applications	185:227	This study investigates polymer fiber-reinforced protein-polysaccharide-based hydrogels for heart valve tissue engineering applications.
32723024	7	68	theme	incorporated	992:1003	arg1	methacrylate					1013:1024	incorporated gelatin methacrylate	992:1024	incorporated gelatin methacrylate	992:1024	Elution studies revealed that less than 20% of incorporated gelatin methacrylate and GAGs were released over 2 weeks, with a steady-state release after the first day.
32723024	4	69	theme	higher	680:685	arg1	porosity					671:678	porosity	671:678	porosity higher than fiber-only conditions	671:712	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC possessed interconnected porous structures and porosity higher than fiber-only conditions.
32723024	10	70	theme	heart	1651:1655	arg1	behavior					1674:1681	heart valve mechanical behavior	1651:1681	heart valve mechanical behavior	1651:1681	Our study demonstrated that compared with the hydrogels and fibers alone, combining both components can yield durable, reinforced composites that mimic heart valve mechanical behavior, while maintaining high cell viability and expressing positive activation as well as proliferation markers.
32723024	5	71	theme	aortic	841:846	arg1	valves					848:853	native porcine aortic valves	826:853	native porcine aortic valves	826:853	These fiber-reinforced HCs exhibited compressive modulus and biaxial mechanical behavior comparable to that of native porcine aortic valves.
32723024	10	72	dep	components	1588:1597	arg1	yield					1603:1607	yield	1603:1607	can yield durable, reinforced composites that mimic heart valve mechanical behavior, while maintaining high cell viability and expressing positive activation	1599:1755	Our study demonstrated that compared with the hydrogels and fibers alone, combining both components can yield durable, reinforced composites that mimic heart valve mechanical behavior, while maintaining high cell viability and expressing positive activation as well as proliferation markers.
32723024	1	73	theme	tissue	197:202	arg1	applications					216:227	heart valve tissue engineering applications	185:227	heart valve tissue engineering applications	185:227	This study investigates polymer fiber-reinforced protein-polysaccharide-based hydrogels for heart valve tissue engineering applications.
32723024	7	74	theme	gelatin	1005:1011	arg1	methacrylate					1013:1024	incorporated gelatin methacrylate	992:1024	incorporated gelatin methacrylate	992:1024	Elution studies revealed that less than 20% of incorporated gelatin methacrylate and GAGs were released over 2 weeks, with a steady-state release after the first day.
32723024	10	75	theme	cell	1707:1710	arg1	viability					1712:1720	high cell viability	1702:1720	high cell viability	1702:1720	Our study demonstrated that compared with the hydrogels and fibers alone, combining both components can yield durable, reinforced composites that mimic heart valve mechanical behavior, while maintaining high cell viability and expressing positive activation as well as proliferation markers.
32723024	9	76	dep	Ki67	1437:1440	arg1	the					1412:1414	the	1412:1414	the	1412:1414	Quiescent VICs expressed alpha smooth muscle actin and calponin showing an activated phenotype, along with a few cells expressing the proliferation marker Ki67 and negative expression for RUNX2, an osteogenic marker.
32723024	1	77	theme	engineering	204:214	arg1	applications					216:227	heart valve tissue engineering applications	185:227	heart valve tissue engineering applications	185:227	This study investigates polymer fiber-reinforced protein-polysaccharide-based hydrogels for heart valve tissue engineering applications.
32723024	4	78	theme	fiber-only	692:701	arg1	conditions					703:712	fiber-only conditions	692:712	fiber-only conditions	692:712	The fiber-reinforced gelatin glycosaminoglycan (GAG)-based HC possessed interconnected porous structures and porosity higher than fiber-only conditions.
32735930	0	0	theme	ultrasonic	92:101	arg1	degradation					103:113	ultrasonic degradation	92:113	ultrasonic degradation	92:113	Changes of digestive and fermentation properties of Sargassum pallidum polysaccharide after ultrasonic degradation and its impacts on gut microbiota.
32735930	4	1	dep	in	740:741	arg1	vitro					743:747	vitro	743:747	vitro	743:747	Furthermore, in vitro fermentation assay indicated that SPP and its degraded fractions showed good fermentability.
32735930	2	2	theme	human	368:372	arg1	saliva					374:379	human saliva	368:379	human saliva	368:379	The results showed that SPP and its degraded fractions were not affected by human saliva, but slightly degraded by breaking glycosidic bonds under simulated gastrointestinal digestion.
32735930	6	3	theme	degraded	1083:1090	arg1	fractions					1092:1100	its degraded fractions	1079:1100	its degraded fractions	1079:1100	SPP and its degraded fractions could modulate gut health by decreasing the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of some beneficial genera, such as Prevotella, Dialister, Phascolarctobacterium, Ruminococcus, and Bacteroides.
32735930	5	4	theme	uronic	941:946	arg1	arabinose					898:906	arabinose	898:906	arabinose	898:906	The predominant compositional monosaccharides including arabinose, galactose, glucose, xylose, and uronic acid were significantly decreased, and the degraded SPP fractions were more easily fermented and utilized by gut bacteria.
32735930	5	4	theme	uronic	941:946	arg1	acid					948:951	uronic acid	941:951	uronic acid	941:951	The predominant compositional monosaccharides including arabinose, galactose, glucose, xylose, and uronic acid were significantly decreased, and the degraded SPP fractions were more easily fermented and utilized by gut bacteria.
32735930	3	5	theme	DPPH	481:484	arg1	activity					505:512	DPPH radical scavenging activity	481:512	DPPH radical scavenging activity	481:512	The DPPH radical scavenging activity, α-glucosidase inhibitory activity, and bile acid-binding capacity of SPP and its degraded fractions were decreased after digestion, which was attributed to the reduction of molecular weights (MWs) and viscosity.
32735930	7	6	theme	microbiota	1424:1433	arg1	community					1435:1443	gut microbiota community	1420:1443	gut microbiota community	1420:1443	These findings suggested that SPP and its degraded fractions exhibited similar influence on gut microbiota community, but appropriate degraded SPP fractions were more easily fermented by gut microbiota.
32735930	3	7	dep	activity	505:512	arg1	The					477:479	The	477:479	The	477:479	The DPPH radical scavenging activity, α-glucosidase inhibitory activity, and bile acid-binding capacity of SPP and its degraded fractions were decreased after digestion, which was attributed to the reduction of molecular weights (MWs) and viscosity.
32735930	5	8	theme	degraded	991:998	arg1	fractions					1004:1012	the degraded SPP fractions	987:1012	the degraded SPP fractions	987:1012	The predominant compositional monosaccharides including arabinose, galactose, glucose, xylose, and uronic acid were significantly decreased, and the degraded SPP fractions were more easily fermented and utilized by gut bacteria.
32735930	3	9	theme	degraded	596:603	arg1	fractions					605:613	its degraded fractions	592:613	its degraded fractions	592:613	The DPPH radical scavenging activity, α-glucosidase inhibitory activity, and bile acid-binding capacity of SPP and its degraded fractions were decreased after digestion, which was attributed to the reduction of molecular weights (MWs) and viscosity.
32735930	6	10	theme	genera	1235:1240	arg1	abundances					1205:1214	the relative abundances	1192:1214	the relative abundances	1192:1214	SPP and its degraded fractions could modulate gut health by decreasing the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of some beneficial genera, such as Prevotella, Dialister, Phascolarctobacterium, Ruminococcus, and Bacteroides.
32735930	6	10	theme	genera	1235:1240	arg1	Ruminococcus					1297:1308	Ruminococcus	1297:1308	Ruminococcus	1297:1308	SPP and its degraded fractions could modulate gut health by decreasing the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of some beneficial genera, such as Prevotella, Dialister, Phascolarctobacterium, Ruminococcus, and Bacteroides.
32735930	6	10	theme	genera	1235:1240	arg1	Bacteroides					1315:1325	Bacteroides	1315:1325	Bacteroides	1315:1325	SPP and its degraded fractions could modulate gut health by decreasing the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of some beneficial genera, such as Prevotella, Dialister, Phascolarctobacterium, Ruminococcus, and Bacteroides.
32735930	6	10	theme	genera	1235:1240	arg1	Phascolarctobacterium					1274:1294	Phascolarctobacterium	1274:1294	Phascolarctobacterium	1274:1294	SPP and its degraded fractions could modulate gut health by decreasing the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of some beneficial genera, such as Prevotella, Dialister, Phascolarctobacterium, Ruminococcus, and Bacteroides.
32735930	6	10	theme	genera	1235:1240	arg1	Dialister					1263:1271	Dialister	1263:1271	Dialister	1263:1271	SPP and its degraded fractions could modulate gut health by decreasing the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of some beneficial genera, such as Prevotella, Dialister, Phascolarctobacterium, Ruminococcus, and Bacteroides.
32735930	3	11	theme	SPP	584:586	arg1	activity					505:512	DPPH radical scavenging activity	481:512	DPPH radical scavenging activity	481:512	The DPPH radical scavenging activity, α-glucosidase inhibitory activity, and bile acid-binding capacity of SPP and its degraded fractions were decreased after digestion, which was attributed to the reduction of molecular weights (MWs) and viscosity.
32735930	3	11	theme	SPP	584:586	arg1	capacity					572:579	bile acid-binding capacity	554:579	bile acid-binding capacity	554:579	The DPPH radical scavenging activity, α-glucosidase inhibitory activity, and bile acid-binding capacity of SPP and its degraded fractions were decreased after digestion, which was attributed to the reduction of molecular weights (MWs) and viscosity.
32735930	3	11	theme	SPP	584:586	arg1	activity					540:547	α-glucosidase inhibitory activity	515:547	α-glucosidase inhibitory activity	515:547	The DPPH radical scavenging activity, α-glucosidase inhibitory activity, and bile acid-binding capacity of SPP and its degraded fractions were decreased after digestion, which was attributed to the reduction of molecular weights (MWs) and viscosity.
32735930	4	12	theme	in	740:741	arg1	assay					762:766	in vitro fermentation assay	740:766	in vitro fermentation assay	740:766	Furthermore, in vitro fermentation assay indicated that SPP and its degraded fractions showed good fermentability.
32735930	5	13	used	utilized	1045:1052	arg2	fractions					1004:1012	the degraded SPP fractions	987:1012	the degraded SPP fractions	987:1012	The predominant compositional monosaccharides including arabinose, galactose, glucose, xylose, and uronic acid were significantly decreased, and the degraded SPP fractions were more easily fermented and utilized by gut bacteria.
32735930	3	14	theme	scavenging	494:503	arg1	activity					505:512	DPPH radical scavenging activity	481:512	DPPH radical scavenging activity	481:512	The DPPH radical scavenging activity, α-glucosidase inhibitory activity, and bile acid-binding capacity of SPP and its degraded fractions were decreased after digestion, which was attributed to the reduction of molecular weights (MWs) and viscosity.
32735930	3	15	theme	acid-binding	559:570	arg1	capacity					572:579	bile acid-binding capacity	554:579	bile acid-binding capacity	554:579	The DPPH radical scavenging activity, α-glucosidase inhibitory activity, and bile acid-binding capacity of SPP and its degraded fractions were decreased after digestion, which was attributed to the reduction of molecular weights (MWs) and viscosity.
32735930	6	16	theme	gut	1117:1119	arg1	health					1121:1126	gut health	1117:1126	gut health	1117:1126	SPP and its degraded fractions could modulate gut health by decreasing the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of some beneficial genera, such as Prevotella, Dialister, Phascolarctobacterium, Ruminococcus, and Bacteroides.
32735930	7	17	theme	gut	1515:1517	arg1	microbiota					1519:1528	gut microbiota	1515:1528	gut microbiota	1515:1528	These findings suggested that SPP and its degraded fractions exhibited similar influence on gut microbiota community, but appropriate degraded SPP fractions were more easily fermented by gut microbiota.
32735930	1	18	theme	fermentation	177:188	arg1	properties					190:199	The in vitro digestive and fermentation properties	150:199	properties	190:199	The in vitro digestive and fermentation properties of Sargassum pallidum polysaccharide (SPP) after ultrasound degradation were investigated.
32735930	2	19	theme	degraded	328:335	arg1	fractions					337:345	its degraded fractions	324:345	its degraded fractions	324:345	The results showed that SPP and its degraded fractions were not affected by human saliva, but slightly degraded by breaking glycosidic bonds under simulated gastrointestinal digestion.
32735930	7	20	theme	degraded	1370:1377	arg1	fractions					1379:1387	its degraded fractions	1366:1387	its degraded fractions	1366:1387	These findings suggested that SPP and its degraded fractions exhibited similar influence on gut microbiota community, but appropriate degraded SPP fractions were more easily fermented by gut microbiota.
32735930	3	21	theme	weights	698:704	arg1	reduction					675:683	the reduction	671:683	the reduction of molecular weights (MWs) and viscosity	671:724	The DPPH radical scavenging activity, α-glucosidase inhibitory activity, and bile acid-binding capacity of SPP and its degraded fractions were decreased after digestion, which was attributed to the reduction of molecular weights (MWs) and viscosity.
32735930	3	22	theme	fractions	605:613	arg1	activity					505:512	DPPH radical scavenging activity	481:512	DPPH radical scavenging activity	481:512	The DPPH radical scavenging activity, α-glucosidase inhibitory activity, and bile acid-binding capacity of SPP and its degraded fractions were decreased after digestion, which was attributed to the reduction of molecular weights (MWs) and viscosity.
32735930	3	22	theme	fractions	605:613	arg1	capacity					572:579	bile acid-binding capacity	554:579	bile acid-binding capacity	554:579	The DPPH radical scavenging activity, α-glucosidase inhibitory activity, and bile acid-binding capacity of SPP and its degraded fractions were decreased after digestion, which was attributed to the reduction of molecular weights (MWs) and viscosity.
32735930	3	22	theme	fractions	605:613	arg1	activity					540:547	α-glucosidase inhibitory activity	515:547	α-glucosidase inhibitory activity	515:547	The DPPH radical scavenging activity, α-glucosidase inhibitory activity, and bile acid-binding capacity of SPP and its degraded fractions were decreased after digestion, which was attributed to the reduction of molecular weights (MWs) and viscosity.
32735930	2	23	theme	gastrointestinal	449:464	arg1	digestion					466:474	simulated gastrointestinal digestion	439:474	simulated gastrointestinal digestion	439:474	The results showed that SPP and its degraded fractions were not affected by human saliva, but slightly degraded by breaking glycosidic bonds under simulated gastrointestinal digestion.
32735930	4	24	theme	fermentation	749:760	arg1	assay					762:766	in vitro fermentation assay	740:766	in vitro fermentation assay	740:766	Furthermore, in vitro fermentation assay indicated that SPP and its degraded fractions showed good fermentability.
32735930	0	25	theme	gut	134:136	arg1	microbiota					138:147	gut microbiota	134:147	gut microbiota	134:147	Changes of digestive and fermentation properties of Sargassum pallidum polysaccharide after ultrasonic degradation and its impacts on gut microbiota.
32735930	6	26	theme	relative	1196:1203	arg1	abundances					1205:1214	the relative abundances	1192:1214	the relative abundances	1192:1214	SPP and its degraded fractions could modulate gut health by decreasing the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of some beneficial genera, such as Prevotella, Dialister, Phascolarctobacterium, Ruminococcus, and Bacteroides.
32735930	6	26	theme	relative	1196:1203	arg1	Ruminococcus					1297:1308	Ruminococcus	1297:1308	Ruminococcus	1297:1308	SPP and its degraded fractions could modulate gut health by decreasing the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of some beneficial genera, such as Prevotella, Dialister, Phascolarctobacterium, Ruminococcus, and Bacteroides.
32735930	6	26	theme	relative	1196:1203	arg1	Bacteroides					1315:1325	Bacteroides	1315:1325	Bacteroides	1315:1325	SPP and its degraded fractions could modulate gut health by decreasing the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of some beneficial genera, such as Prevotella, Dialister, Phascolarctobacterium, Ruminococcus, and Bacteroides.
32735930	6	26	theme	relative	1196:1203	arg1	Phascolarctobacterium					1274:1294	Phascolarctobacterium	1274:1294	Phascolarctobacterium	1274:1294	SPP and its degraded fractions could modulate gut health by decreasing the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of some beneficial genera, such as Prevotella, Dialister, Phascolarctobacterium, Ruminococcus, and Bacteroides.
32735930	6	26	theme	relative	1196:1203	arg1	Dialister					1263:1271	Dialister	1263:1271	Dialister	1263:1271	SPP and its degraded fractions could modulate gut health by decreasing the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of some beneficial genera, such as Prevotella, Dialister, Phascolarctobacterium, Ruminococcus, and Bacteroides.
32735930	1	27	theme	Sargassum	204:212	arg1	SPP					239:241	SPP	239:241	SPP	239:241	The in vitro digestive and fermentation properties of Sargassum pallidum polysaccharide (SPP) after ultrasound degradation were investigated.
32735930	1	27	theme	Sargassum	204:212	arg1	polysaccharide					223:236	Sargassum pallidum polysaccharide	204:236	Sargassum pallidum polysaccharide (SPP) after ultrasound degradation	204:271	The in vitro digestive and fermentation properties of Sargassum pallidum polysaccharide (SPP) after ultrasound degradation were investigated.
32735930	2	28	theme	simulated	439:447	arg1	digestion					466:474	simulated gastrointestinal digestion	439:474	simulated gastrointestinal digestion	439:474	The results showed that SPP and its degraded fractions were not affected by human saliva, but slightly degraded by breaking glycosidic bonds under simulated gastrointestinal digestion.
32735930	4	29	theme	degraded	795:802	arg1	fractions					804:812	its degraded fractions	791:812	its degraded fractions	791:812	Furthermore, in vitro fermentation assay indicated that SPP and its degraded fractions showed good fermentability.
32735930	1	30	dep	in	154:155	arg1	vitro					157:161	vitro	157:161	vitro	157:161	The in vitro digestive and fermentation properties of Sargassum pallidum polysaccharide (SPP) after ultrasound degradation were investigated.
32735930	1	31	theme	pallidum	214:221	arg1	SPP					239:241	SPP	239:241	SPP	239:241	The in vitro digestive and fermentation properties of Sargassum pallidum polysaccharide (SPP) after ultrasound degradation were investigated.
32735930	1	31	theme	pallidum	214:221	arg1	polysaccharide					223:236	Sargassum pallidum polysaccharide	204:236	Sargassum pallidum polysaccharide (SPP) after ultrasound degradation	204:271	The in vitro digestive and fermentation properties of Sargassum pallidum polysaccharide (SPP) after ultrasound degradation were investigated.
32735930	0	32	theme	properties	38:47	arg1	Changes					0:6	Changes	0:6	Changes of digestive and fermentation properties of Sargassum pallidum polysaccharide after ultrasonic degradation and its impacts on gut microbiota.	0:148	Changes of digestive and fermentation properties of Sargassum pallidum polysaccharide after ultrasonic degradation and its impacts on gut microbiota.
32735930	1	33	theme	polysaccharide	223:236	arg1	properties					190:199	The in vitro digestive and fermentation properties	150:199	properties	190:199	The in vitro digestive and fermentation properties of Sargassum pallidum polysaccharide (SPP) after ultrasound degradation were investigated.
32735930	1	33	theme	polysaccharide	223:236	arg1	digestive					163:171	The in vitro digestive and fermentation properties	150:199	digestive	163:171	The in vitro digestive and fermentation properties of Sargassum pallidum polysaccharide (SPP) after ultrasound degradation were investigated.
32735930	0	34	dep	Sargassum	52:60	arg1	pallidum					62:69	pallidum	62:69	pallidum	62:69	Changes of digestive and fermentation properties of Sargassum pallidum polysaccharide after ultrasonic degradation and its impacts on gut microbiota.
32735930	2	35	theme	glycosidic	416:425	arg1	bonds					427:431	breaking glycosidic bonds	407:431	breaking glycosidic bonds	407:431	The results showed that SPP and its degraded fractions were not affected by human saliva, but slightly degraded by breaking glycosidic bonds under simulated gastrointestinal digestion.
32735930	0	36	theme	fermentation	25:36	arg1	properties					38:47	fermentation properties	25:47	fermentation properties	25:47	Changes of digestive and fermentation properties of Sargassum pallidum polysaccharide after ultrasonic degradation and its impacts on gut microbiota.
32735930	7	37	theme	degraded	1462:1469	arg1	fractions					1475:1483	appropriate degraded SPP fractions	1450:1483	appropriate degraded SPP fractions	1450:1483	These findings suggested that SPP and its degraded fractions exhibited similar influence on gut microbiota community, but appropriate degraded SPP fractions were more easily fermented by gut microbiota.
32735930	5	38	theme	gut	1057:1059	arg1	bacteria					1061:1068	gut bacteria	1057:1068	gut bacteria	1057:1068	The predominant compositional monosaccharides including arabinose, galactose, glucose, xylose, and uronic acid were significantly decreased, and the degraded SPP fractions were more easily fermented and utilized by gut bacteria.
32735930	2	39	theme	breaking	407:414	arg1	bonds					427:431	breaking glycosidic bonds	407:431	breaking glycosidic bonds	407:431	The results showed that SPP and its degraded fractions were not affected by human saliva, but slightly degraded by breaking glycosidic bonds under simulated gastrointestinal digestion.
32735930	0	40	theme	Sargassum	52:60	arg1	polysaccharide					71:84	Sargassum pallidum polysaccharide	52:84	Sargassum pallidum polysaccharide	52:84	Changes of digestive and fermentation properties of Sargassum pallidum polysaccharide after ultrasonic degradation and its impacts on gut microbiota.
32735930	5	41	theme	compositional	858:870	arg1	arabinose					898:906	arabinose	898:906	arabinose	898:906	The predominant compositional monosaccharides including arabinose, galactose, glucose, xylose, and uronic acid were significantly decreased, and the degraded SPP fractions were more easily fermented and utilized by gut bacteria.
32735930	5	41	theme	compositional	858:870	arg1	monosaccharides					872:886	The predominant compositional monosaccharides	842:886	The predominant compositional monosaccharides including arabinose, galactose, glucose, xylose, and uronic acid	842:951	The predominant compositional monosaccharides including arabinose, galactose, glucose, xylose, and uronic acid were significantly decreased, and the degraded SPP fractions were more easily fermented and utilized by gut bacteria.
32735930	3	42	theme	α-glucosidase	515:527	arg1	activity					540:547	α-glucosidase inhibitory activity	515:547	α-glucosidase inhibitory activity	515:547	The DPPH radical scavenging activity, α-glucosidase inhibitory activity, and bile acid-binding capacity of SPP and its degraded fractions were decreased after digestion, which was attributed to the reduction of molecular weights (MWs) and viscosity.
32735930	0	43	from	impacts	123:129	arg1	microbiota					138:147	gut microbiota	134:147	gut microbiota	134:147	Changes of digestive and fermentation properties of Sargassum pallidum polysaccharide after ultrasonic degradation and its impacts on gut microbiota.
32735930	5	44	theme	predominant	846:856	arg1	arabinose					898:906	arabinose	898:906	arabinose	898:906	The predominant compositional monosaccharides including arabinose, galactose, glucose, xylose, and uronic acid were significantly decreased, and the degraded SPP fractions were more easily fermented and utilized by gut bacteria.
32735930	5	44	theme	predominant	846:856	arg1	monosaccharides					872:886	The predominant compositional monosaccharides	842:886	The predominant compositional monosaccharides including arabinose, galactose, glucose, xylose, and uronic acid	842:951	The predominant compositional monosaccharides including arabinose, galactose, glucose, xylose, and uronic acid were significantly decreased, and the degraded SPP fractions were more easily fermented and utilized by gut bacteria.
32735930	7	45	theme	similar	1399:1405	arg1	influence					1407:1415	similar influence	1399:1415	similar influence	1399:1415	These findings suggested that SPP and its degraded fractions exhibited similar influence on gut microbiota community, but appropriate degraded SPP fractions were more easily fermented by gut microbiota.
32735930	3	46	theme	inhibitory	529:538	arg1	activity					540:547	α-glucosidase inhibitory activity	515:547	α-glucosidase inhibitory activity	515:547	The DPPH radical scavenging activity, α-glucosidase inhibitory activity, and bile acid-binding capacity of SPP and its degraded fractions were decreased after digestion, which was attributed to the reduction of molecular weights (MWs) and viscosity.
32735930	4	47	theme	good	821:824	arg1	fermentability					826:839	good fermentability	821:839	good fermentability	821:839	Furthermore, in vitro fermentation assay indicated that SPP and its degraded fractions showed good fermentability.
32735930	7	48	theme	gut	1420:1422	arg1	community					1435:1443	gut microbiota community	1420:1443	gut microbiota community	1420:1443	These findings suggested that SPP and its degraded fractions exhibited similar influence on gut microbiota community, but appropriate degraded SPP fractions were more easily fermented by gut microbiota.
32735930	7	49	theme	appropriate	1450:1460	arg1	fractions					1475:1483	appropriate degraded SPP fractions	1450:1483	appropriate degraded SPP fractions	1450:1483	These findings suggested that SPP and its degraded fractions exhibited similar influence on gut microbiota community, but appropriate degraded SPP fractions were more easily fermented by gut microbiota.
32735930	6	50	theme	Firmicutes/Bacteroidetes	1146:1169	arg1	ratio					1171:1175	the Firmicutes/Bacteroidetes ratio	1142:1175	the Firmicutes/Bacteroidetes ratio	1142:1175	SPP and its degraded fractions could modulate gut health by decreasing the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of some beneficial genera, such as Prevotella, Dialister, Phascolarctobacterium, Ruminococcus, and Bacteroides.
32735930	7	51	theme	SPP	1471:1473	arg1	fractions					1475:1483	appropriate degraded SPP fractions	1450:1483	appropriate degraded SPP fractions	1450:1483	These findings suggested that SPP and its degraded fractions exhibited similar influence on gut microbiota community, but appropriate degraded SPP fractions were more easily fermented by gut microbiota.
32735930	3	52	theme	molecular	688:696	arg1	weights					698:704	molecular weights	688:704	molecular weights (MWs)	688:710	The DPPH radical scavenging activity, α-glucosidase inhibitory activity, and bile acid-binding capacity of SPP and its degraded fractions were decreased after digestion, which was attributed to the reduction of molecular weights (MWs) and viscosity.
32735930	3	52	theme	molecular	688:696	arg1	MWs					707:709	MWs	707:709	MWs	707:709	The DPPH radical scavenging activity, α-glucosidase inhibitory activity, and bile acid-binding capacity of SPP and its degraded fractions were decreased after digestion, which was attributed to the reduction of molecular weights (MWs) and viscosity.
32735930	5	53	theme	SPP	1000:1002	arg1	fractions					1004:1012	the degraded SPP fractions	987:1012	the degraded SPP fractions	987:1012	The predominant compositional monosaccharides including arabinose, galactose, glucose, xylose, and uronic acid were significantly decreased, and the degraded SPP fractions were more easily fermented and utilized by gut bacteria.
32735930	3	54	theme	bile	554:557	arg1	capacity					572:579	bile acid-binding capacity	554:579	bile acid-binding capacity	554:579	The DPPH radical scavenging activity, α-glucosidase inhibitory activity, and bile acid-binding capacity of SPP and its degraded fractions were decreased after digestion, which was attributed to the reduction of molecular weights (MWs) and viscosity.
32735930	0	55	theme	polysaccharide	71:84	arg1	digestive					11:19	digestive	11:19	digestive	11:19	Changes of digestive and fermentation properties of Sargassum pallidum polysaccharide after ultrasonic degradation and its impacts on gut microbiota.
32735930	0	55	theme	polysaccharide	71:84	arg1	properties					38:47	fermentation properties	25:47	fermentation properties	25:47	Changes of digestive and fermentation properties of Sargassum pallidum polysaccharide after ultrasonic degradation and its impacts on gut microbiota.
32735930	0	56	from	degradation	103:113	arg1	microbiota					138:147	gut microbiota	134:147	gut microbiota	134:147	Changes of digestive and fermentation properties of Sargassum pallidum polysaccharide after ultrasonic degradation and its impacts on gut microbiota.
32735930	3	57	theme	radical	486:492	arg1	activity					505:512	DPPH radical scavenging activity	481:512	DPPH radical scavenging activity	481:512	The DPPH radical scavenging activity, α-glucosidase inhibitory activity, and bile acid-binding capacity of SPP and its degraded fractions were decreased after digestion, which was attributed to the reduction of molecular weights (MWs) and viscosity.
32735930	6	58	theme	beneficial	1224:1233	arg1	genera					1235:1240	some beneficial genera	1219:1240	some beneficial genera	1219:1240	SPP and its degraded fractions could modulate gut health by decreasing the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of some beneficial genera, such as Prevotella, Dialister, Phascolarctobacterium, Ruminococcus, and Bacteroides.
32735930	3	59	theme	viscosity	716:724	arg1	reduction					675:683	the reduction	671:683	the reduction of molecular weights (MWs) and viscosity	671:724	The DPPH radical scavenging activity, α-glucosidase inhibitory activity, and bile acid-binding capacity of SPP and its degraded fractions were decreased after digestion, which was attributed to the reduction of molecular weights (MWs) and viscosity.
32735930	0	60	theme	digestive	11:19	arg1	Changes					0:6	Changes	0:6	Changes of digestive and fermentation properties of Sargassum pallidum polysaccharide after ultrasonic degradation and its impacts on gut microbiota.	0:148	Changes of digestive and fermentation properties of Sargassum pallidum polysaccharide after ultrasonic degradation and its impacts on gut microbiota.
32735930	1	61	theme	in	154:155	arg1	digestive					163:171	The in vitro digestive and fermentation properties	150:199	digestive	163:171	The in vitro digestive and fermentation properties of Sargassum pallidum polysaccharide (SPP) after ultrasound degradation were investigated.
32735930	1	62	theme	ultrasound	250:259	arg1	degradation					261:271	ultrasound degradation	250:271	ultrasound degradation	250:271	The in vitro digestive and fermentation properties of Sargassum pallidum polysaccharide (SPP) after ultrasound degradation were investigated.
34651560	12	0	dep	ciprofloxacin	1648:1660	arg1	standard					1663:1670	standard	1663:1670	standard	1663:1670	Silver-ciprofloxacin nano-composites exhibited comparable activity (zone of inhibition 19-30 mm) against E. coli to that of ciprofloxacin (standard, 21-35 mm) and relatively lower activity in case of S. aureus (zone of inhabitation 11-17 mm).
34651560	2	1	with	Lack	241:244	arg1	wound					304:308	the wound	300:308	the wound	300:308	Lack of absorptive potential and sticking of dressing with the wound causes pain and slows the healing process.
34651560	4	2	theme	wound	559:563	arg1	dressing					565:572	a wound dressing	557:572	a wound dressing	557:572	The hemicelluloses from Lallemantia royleana seeds possess desirable properties for a wound dressing.
34651560	7	3	theme	electron	930:937	arg1	microscopy					939:948	scanning electron microscopy	921:948	scanning electron microscopy	921:948	The composites were characterized by elemental analysis, Fourier-transform infrared spectroscopy and scanning electron microscopy, and evaluated for their antibacterial activity against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive).
34651560	7	3	theme	electron	930:937	arg1	analysis					867:874	elemental analysis	857:874	elemental analysis	857:874	The composites were characterized by elemental analysis, Fourier-transform infrared spectroscopy and scanning electron microscopy, and evaluated for their antibacterial activity against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive).
34651560	8	4	dep	in	1114:1115	arg1	vivo					1117:1120	vivo	1117:1120	vivo	1117:1120	The dressings were subjected to in vivo studies on Albino rats.
34651560	8	5	theme	in	1114:1115	arg1	studies					1122:1128	in vivo studies	1114:1128	in vivo studies on Albino rats	1114:1143	The dressings were subjected to in vivo studies on Albino rats.
34651560	14	6	theme	improved	1979:1986	arg1	properties					1988:1997	improved properties	1979:1997	improved properties because of having high swelling index, porosity and spongy texture	1979:2064	Thus the use of hemicellulose from L. royleana seeds proved to be beneficial for preparing wound dressings with improved properties because of having high swelling index, porosity and spongy texture.
34651560	7	7	dep	coli	1018:1021	arg1	Gram-negative					1024:1036	Gram-negative	1024:1036	Gram-negative	1024:1036	The composites were characterized by elemental analysis, Fourier-transform infrared spectroscopy and scanning electron microscopy, and evaluated for their antibacterial activity against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive).
34651560	12	8	dep	activity	1582:1589	arg1	zone					1592:1595	zone	1592:1595	zone of inhibition 19-30 mm	1592:1618	Silver-ciprofloxacin nano-composites exhibited comparable activity (zone of inhibition 19-30 mm) against E. coli to that of ciprofloxacin (standard, 21-35 mm) and relatively lower activity in case of S. aureus (zone of inhabitation 11-17 mm).
34651560	5	9	theme	absorptive	660:669	arg1	properties					671:680	the absorptive properties	656:680	the absorptive properties of the hemicellulose	656:701	The hemicellulose was blended with chitosan/chitin and glutaraldehyde to enhance the absorptive properties of the hemicellulose through cross-linking.
34651560	14	10	from	seeds	1914:1918	arg1	use					1876:1878	the use	1872:1878	the use of hemicellulose from L. royleana seeds	1872:1918	Thus the use of hemicellulose from L. royleana seeds proved to be beneficial for preparing wound dressings with improved properties because of having high swelling index, porosity and spongy texture.
34651560	14	10	from	seeds	1914:1918	arg1	beneficial					1933:1942	beneficial	1933:1942	beneficial	1933:1942	Thus the use of hemicellulose from L. royleana seeds proved to be beneficial for preparing wound dressings with improved properties because of having high swelling index, porosity and spongy texture.
34651560	14	10	from	seeds	1914:1918	arg1	hemicellulose					1883:1895	hemicellulose	1883:1895	hemicellulose from L. royleana seeds	1883:1918	Thus the use of hemicellulose from L. royleana seeds proved to be beneficial for preparing wound dressings with improved properties because of having high swelling index, porosity and spongy texture.
34651560	14	11	theme	spongy	2051:2056	arg1	texture					2058:2064	spongy texture	2051:2064	spongy texture	2051:2064	Thus the use of hemicellulose from L. royleana seeds proved to be beneficial for preparing wound dressings with improved properties because of having high swelling index, porosity and spongy texture.
34651560	9	12	theme	silver	1192:1197	arg1	nanoparticles					1199:1211	the silver nanoparticles	1188:1211	the silver nanoparticles	1188:1211	The dressings were found to be porous and the silver nanoparticles and drug particles were found to be uniformly distributed in the polymeric matrix.
34651560	2	13	theme	potential	260:268	arg1	sticking					274:281	sticking	274:281	sticking	274:281	Lack of absorptive potential and sticking of dressing with the wound causes pain and slows the healing process.
34651560	2	13	theme	potential	260:268	arg1	Lack					241:244	Lack	241:244	Lack of absorptive potential	241:268	Lack of absorptive potential and sticking of dressing with the wound causes pain and slows the healing process.
34651560	7	14	theme	scanning	921:928	arg1	microscopy					939:948	scanning electron microscopy	921:948	scanning electron microscopy	921:948	The composites were characterized by elemental analysis, Fourier-transform infrared spectroscopy and scanning electron microscopy, and evaluated for their antibacterial activity against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive).
34651560	7	14	theme	scanning	921:928	arg1	analysis					867:874	elemental analysis	857:874	elemental analysis	857:874	The composites were characterized by elemental analysis, Fourier-transform infrared spectroscopy and scanning electron microscopy, and evaluated for their antibacterial activity against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive).
34651560	11	15	theme	data	1417:1420	arg1	extrapolation					1396:1408	extrapolation	1396:1408	extrapolation of the data	1396:1420	From extrapolation of the data, it was discovered that the formulation would release around 80% of ciprofloxacin in about two weeks.
34651560	0	16	theme	wound	111:115	arg1	dressings					117:125	wound dressings	111:125	wound dressings formed from Lallemantia royleana seeds' mucilage	111:174	Preparation, characterization and biological evaluation of silver nanoparticles and drug loaded composites for wound dressings formed from Lallemantia royleana seeds' mucilage.
34651560	14	17	with	dressings	1964:1972	arg1	properties					1988:1997	improved properties	1979:1997	improved properties because of having high swelling index, porosity and spongy texture	1979:2064	Thus the use of hemicellulose from L. royleana seeds proved to be beneficial for preparing wound dressings with improved properties because of having high swelling index, porosity and spongy texture.
34651560	3	18	with	sticking	448:455	arg1	wound					466:470	the wound	462:470	the wound	462:470	The aim of this study was to develop wound dressings having more absorptive potential and less sticking with the wound.
34651560	12	19	theme	inhibition	1600:1609	arg1	zone					1592:1595	zone	1592:1595	zone of inhibition 19-30 mm	1592:1618	Silver-ciprofloxacin nano-composites exhibited comparable activity (zone of inhibition 19-30 mm) against E. coli to that of ciprofloxacin (standard, 21-35 mm) and relatively lower activity in case of S. aureus (zone of inhabitation 11-17 mm).
34651560	2	20	theme	absorptive	249:258	arg1	potential					260:268	absorptive potential	249:268	absorptive potential	249:268	Lack of absorptive potential and sticking of dressing with the wound causes pain and slows the healing process.
34651560	7	21	theme	infrared	895:902	arg1	spectroscopy					904:915	Fourier-transform infrared spectroscopy	877:915	Fourier-transform infrared spectroscopy	877:915	The composites were characterized by elemental analysis, Fourier-transform infrared spectroscopy and scanning electron microscopy, and evaluated for their antibacterial activity against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive).
34651560	7	21	theme	infrared	895:902	arg1	analysis					867:874	elemental analysis	857:874	elemental analysis	857:874	The composites were characterized by elemental analysis, Fourier-transform infrared spectroscopy and scanning electron microscopy, and evaluated for their antibacterial activity against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive).
34651560	12	22	dep	standard	1663:1670	arg1	21-35 mm					1673:1680	21-35 mm	1673:1680	21-35 mm	1673:1680	Silver-ciprofloxacin nano-composites exhibited comparable activity (zone of inhibition 19-30 mm) against E. coli to that of ciprofloxacin (standard, 21-35 mm) and relatively lower activity in case of S. aureus (zone of inhabitation 11-17 mm).
34651560	8	23	theme	Albino	1133:1138	arg1	rats					1140:1143	Albino rats	1133:1143	Albino rats	1133:1143	The dressings were subjected to in vivo studies on Albino rats.
34651560	14	24	theme	swelling	2022:2029	arg1	index					2031:2035	high swelling index	2017:2035	high swelling index	2017:2035	Thus the use of hemicellulose from L. royleana seeds proved to be beneficial for preparing wound dressings with improved properties because of having high swelling index, porosity and spongy texture.
34651560	4	25	theme	desirable	532:540	arg1	properties					542:551	desirable properties	532:551	desirable properties for a wound dressing	532:572	The hemicelluloses from Lallemantia royleana seeds possess desirable properties for a wound dressing.
34651560	14	26	theme	high	2017:2020	arg1	index					2031:2035	high swelling index	2017:2035	high swelling index	2017:2035	Thus the use of hemicellulose from L. royleana seeds proved to be beneficial for preparing wound dressings with improved properties because of having high swelling index, porosity and spongy texture.
34651560	13	27	theme	healing	1850:1856	arg1	process					1858:1864	the healing process	1846:1864	the healing process	1846:1864	The dressings did not stick to the wound site and the site remained wet during the healing process.
34651560	3	28	theme	wound	390:394	arg1	dressings					396:404	wound dressings	390:404	wound dressings having more absorptive potential and less sticking with the wound	390:470	The aim of this study was to develop wound dressings having more absorptive potential and less sticking with the wound.
34651560	12	29	dep	aureus	1727:1732	arg1	zone					1735:1738	zone	1735:1738	zone of inhabitation 11-17 mm	1735:1763	Silver-ciprofloxacin nano-composites exhibited comparable activity (zone of inhibition 19-30 mm) against E. coli to that of ciprofloxacin (standard, 21-35 mm) and relatively lower activity in case of S. aureus (zone of inhabitation 11-17 mm).
34651560	4	30	contain	possess	524:530	arg2	properties					542:551	desirable properties	532:551	desirable properties for a wound dressing	532:572	The hemicelluloses from Lallemantia royleana seeds possess desirable properties for a wound dressing.
34651560	4	30	contain	possess	524:530	arg1	hemicelluloses					477:490	The hemicelluloses	473:490	The hemicelluloses from Lallemantia royleana seeds	473:522	The hemicelluloses from Lallemantia royleana seeds possess desirable properties for a wound dressing.
34651560	7	31	theme	Fourier-transform	877:893	arg1	spectroscopy					904:915	Fourier-transform infrared spectroscopy	877:915	Fourier-transform infrared spectroscopy	877:915	The composites were characterized by elemental analysis, Fourier-transform infrared spectroscopy and scanning electron microscopy, and evaluated for their antibacterial activity against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive).
34651560	7	31	theme	Fourier-transform	877:893	arg1	analysis					867:874	elemental analysis	857:874	elemental analysis	857:874	The composites were characterized by elemental analysis, Fourier-transform infrared spectroscopy and scanning electron microscopy, and evaluated for their antibacterial activity against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive).
34651560	13	32	theme	wound	1802:1806	arg1	site					1808:1811	the wound site	1798:1811	the wound site	1798:1811	The dressings did not stick to the wound site and the site remained wet during the healing process.
34651560	9	33	theme	polymeric	1278:1286	arg1	matrix					1288:1293	the polymeric matrix	1274:1293	the polymeric matrix	1274:1293	The dressings were found to be porous and the silver nanoparticles and drug particles were found to be uniformly distributed in the polymeric matrix.
34651560	12	34	theme	lower	1698:1702	arg1	activity					1704:1711	relatively lower activity	1687:1711	relatively lower activity in case of S. aureus (zone of inhabitation 11-17 mm)	1687:1764	Silver-ciprofloxacin nano-composites exhibited comparable activity (zone of inhibition 19-30 mm) against E. coli to that of ciprofloxacin (standard, 21-35 mm) and relatively lower activity in case of S. aureus (zone of inhabitation 11-17 mm).
34651560	7	35	theme	elemental	857:865	arg1	spectroscopy					904:915	Fourier-transform infrared spectroscopy	877:915	Fourier-transform infrared spectroscopy	877:915	The composites were characterized by elemental analysis, Fourier-transform infrared spectroscopy and scanning electron microscopy, and evaluated for their antibacterial activity against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive).
34651560	7	35	theme	elemental	857:865	arg1	analysis					867:874	elemental analysis	857:874	elemental analysis	857:874	The composites were characterized by elemental analysis, Fourier-transform infrared spectroscopy and scanning electron microscopy, and evaluated for their antibacterial activity against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive).
34651560	7	35	theme	elemental	857:865	arg1	microscopy					939:948	scanning electron microscopy	921:948	scanning electron microscopy	921:948	The composites were characterized by elemental analysis, Fourier-transform infrared spectroscopy and scanning electron microscopy, and evaluated for their antibacterial activity against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive).
34651560	14	36	theme	royleana	1905:1912	arg1	seeds					1914:1918	L. royleana seeds	1902:1918	L. royleana seeds	1902:1918	Thus the use of hemicellulose from L. royleana seeds proved to be beneficial for preparing wound dressings with improved properties because of having high swelling index, porosity and spongy texture.
34651560	4	37	from	seeds	518:522	arg1	hemicelluloses					477:490	The hemicelluloses	473:490	The hemicelluloses from Lallemantia royleana seeds	473:522	The hemicelluloses from Lallemantia royleana seeds possess desirable properties for a wound dressing.
34651560	11	38	theme	ciprofloxacin	1490:1502	arg1	ciprofloxacin					1490:1502	ciprofloxacin	1490:1502	ciprofloxacin	1490:1502	From extrapolation of the data, it was discovered that the formulation would release around 80% of ciprofloxacin in about two weeks.
34651560	11	38	theme	ciprofloxacin	1490:1502	arg1	%					1485:1485	80%	1483:1485	80% of ciprofloxacin	1483:1502	From extrapolation of the data, it was discovered that the formulation would release around 80% of ciprofloxacin in about two weeks.
34651560	0	39	theme	royleana	151:158	arg1	seeds					160:164	Lallemantia royleana seeds'	139:165	Lallemantia royleana seeds' mucilage	139:174	Preparation, characterization and biological evaluation of silver nanoparticles and drug loaded composites for wound dressings formed from Lallemantia royleana seeds' mucilage.
34651560	10	40	theme	containing	1310:1319	arg1	ciprofloxacin					1321:1333	The composite containing ciprofloxacin	1296:1333	The composite containing ciprofloxacin	1296:1333	The composite containing ciprofloxacin released the drug in a sustained manner for 14-16 days.
34651560	10	41	from	drug	1348:1351	arg1	manner					1368:1373	a sustained manner	1356:1373	a sustained manner	1356:1373	The composite containing ciprofloxacin released the drug in a sustained manner for 14-16 days.
34651560	10	42	theme	sustained	1358:1366	arg1	manner					1368:1373	a sustained manner	1356:1373	a sustained manner	1356:1373	The composite containing ciprofloxacin released the drug in a sustained manner for 14-16 days.
34651560	14	43	theme	L.	1902:1903	arg1	seeds					1914:1918	L. royleana seeds	1902:1918	L. royleana seeds	1902:1918	Thus the use of hemicellulose from L. royleana seeds proved to be beneficial for preparing wound dressings with improved properties because of having high swelling index, porosity and spongy texture.
34651560	0	44	theme	biological	34:43	arg1	evaluation					45:54	biological evaluation	34:54	biological evaluation	34:54	Preparation, characterization and biological evaluation of silver nanoparticles and drug loaded composites for wound dressings formed from Lallemantia royleana seeds' mucilage.
34651560	4	45	theme	Lallemantia	497:507	arg1	seeds					518:522	Lallemantia royleana seeds	497:522	Lallemantia royleana seeds	497:522	The hemicelluloses from Lallemantia royleana seeds possess desirable properties for a wound dressing.
34651560	0	46	theme	Lallemantia	139:149	arg1	seeds					160:164	Lallemantia royleana seeds'	139:165	Lallemantia royleana seeds' mucilage	139:174	Preparation, characterization and biological evaluation of silver nanoparticles and drug loaded composites for wound dressings formed from Lallemantia royleana seeds' mucilage.
34651560	3	47	theme	absorptive	418:427	arg1	potential					429:437	more absorptive potential	413:437	more absorptive potential	413:437	The aim of this study was to develop wound dressings having more absorptive potential and less sticking with the wound.
34651560	0	48	theme	silver	59:64	arg1	nanoparticles					66:78	silver nanoparticles	59:78	silver nanoparticles	59:78	Preparation, characterization and biological evaluation of silver nanoparticles and drug loaded composites for wound dressings formed from Lallemantia royleana seeds' mucilage.
34651560	3	49	dep	dressings	396:404	arg1	having					406:411	having	406:411	having more absorptive potential	406:437	The aim of this study was to develop wound dressings having more absorptive potential and less sticking with the wound.
34651560	3	49	dep	dressings	396:404	arg1	sticking					448:455	sticking	448:455	sticking	448:455	The aim of this study was to develop wound dressings having more absorptive potential and less sticking with the wound.
34651560	14	50	theme	hemicellulose	1883:1895	arg1	use					1876:1878	the use	1872:1878	the use of hemicellulose from L. royleana seeds	1872:1918	Thus the use of hemicellulose from L. royleana seeds proved to be beneficial for preparing wound dressings with improved properties because of having high swelling index, porosity and spongy texture.
34651560	14	50	theme	hemicellulose	1883:1895	arg1	beneficial					1933:1942	beneficial	1933:1942	beneficial	1933:1942	Thus the use of hemicellulose from L. royleana seeds proved to be beneficial for preparing wound dressings with improved properties because of having high swelling index, porosity and spongy texture.
34651560	3	51	theme	study	369:373	arg1	aim					357:359	The aim	353:359	The aim of this study	353:373	The aim of this study was to develop wound dressings having more absorptive potential and less sticking with the wound.
34651560	8	52	from	studies	1122:1128	arg1	rats					1140:1143	Albino rats	1133:1143	Albino rats	1133:1143	The dressings were subjected to in vivo studies on Albino rats.
34651560	12	53	theme	Silver-ciprofloxacin	1524:1543	arg1	nano-composites					1545:1559	Silver-ciprofloxacin nano-composites	1524:1559	Silver-ciprofloxacin nano-composites	1524:1559	Silver-ciprofloxacin nano-composites exhibited comparable activity (zone of inhibition 19-30 mm) against E. coli to that of ciprofloxacin (standard, 21-35 mm) and relatively lower activity in case of S. aureus (zone of inhabitation 11-17 mm).
34651560	14	54	from	use	1876:1878	arg1	seeds					1914:1918	L. royleana seeds	1902:1918	L. royleana seeds	1902:1918	Thus the use of hemicellulose from L. royleana seeds proved to be beneficial for preparing wound dressings with improved properties because of having high swelling index, porosity and spongy texture.
34651560	12	55	theme	11-17 mm	1756:1763	arg1	zone					1735:1738	zone	1735:1738	zone of inhabitation 11-17 mm	1735:1763	Silver-ciprofloxacin nano-composites exhibited comparable activity (zone of inhibition 19-30 mm) against E. coli to that of ciprofloxacin (standard, 21-35 mm) and relatively lower activity in case of S. aureus (zone of inhabitation 11-17 mm).
34651560	4	56	theme	royleana	509:516	arg1	seeds					518:522	Lallemantia royleana seeds	497:522	Lallemantia royleana seeds	497:522	The hemicelluloses from Lallemantia royleana seeds possess desirable properties for a wound dressing.
34651560	6	57	theme	silver	766:771	arg1	nanoparticles					773:785	silver nanoparticles	766:785	silver nanoparticles	766:785	Two types of formulations incorporating silver nanoparticles and ciprofloxacin were prepared.
34651560	2	58	theme	dressing	286:293	arg1	sticking					274:281	sticking	274:281	sticking	274:281	Lack of absorptive potential and sticking of dressing with the wound causes pain and slows the healing process.
34651560	2	58	theme	dressing	286:293	arg1	Lack					241:244	Lack	241:244	Lack of absorptive potential	241:268	Lack of absorptive potential and sticking of dressing with the wound causes pain and slows the healing process.
34651560	5	59	theme	hemicellulose	689:701	arg1	properties					671:680	the absorptive properties	656:680	the absorptive properties of the hemicellulose	656:701	The hemicellulose was blended with chitosan/chitin and glutaraldehyde to enhance the absorptive properties of the hemicellulose through cross-linking.
34651560	14	60	theme	wound	1958:1962	arg1	dressings					1964:1972	wound dressings	1958:1972	wound dressings with improved properties because of having high swelling index, porosity and spongy texture	1958:2064	Thus the use of hemicellulose from L. royleana seeds proved to be beneficial for preparing wound dressings with improved properties because of having high swelling index, porosity and spongy texture.
34651560	12	61	theme	comparable	1571:1580	arg1	activity					1582:1589	comparable activity	1571:1589	comparable activity (zone of inhibition 19-30 mm) against E. coli to that of ciprofloxacin (standard, 21-35 mm)	1571:1681	Silver-ciprofloxacin nano-composites exhibited comparable activity (zone of inhibition 19-30 mm) against E. coli to that of ciprofloxacin (standard, 21-35 mm) and relatively lower activity in case of S. aureus (zone of inhabitation 11-17 mm).
34651560	10	62	theme	composite	1300:1308	arg1	ciprofloxacin					1321:1333	The composite containing ciprofloxacin	1296:1333	The composite containing ciprofloxacin	1296:1333	The composite containing ciprofloxacin released the drug in a sustained manner for 14-16 days.
34651560	6	63	theme	formulations	739:750	arg1	types					730:734	Two types	726:734	Two types of formulations incorporating silver nanoparticles and ciprofloxacin	726:803	Two types of formulations incorporating silver nanoparticles and ciprofloxacin were prepared.
34651560	7	64	theme	antibacterial	975:987	arg1	activity					989:996	their antibacterial activity	969:996	their antibacterial activity against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive)	969:1079	The composites were characterized by elemental analysis, Fourier-transform infrared spectroscopy and scanning electron microscopy, and evaluated for their antibacterial activity against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive).
34651560	2	65	with	sticking	274:281	arg1	wound					304:308	the wound	300:308	the wound	300:308	Lack of absorptive potential and sticking of dressing with the wound causes pain and slows the healing process.
34651560	0	66	theme	nanoparticles	66:78	arg1	drug					84:87	drug	84:87	drug	84:87	Preparation, characterization and biological evaluation of silver nanoparticles and drug loaded composites for wound dressings formed from Lallemantia royleana seeds' mucilage.
34651560	0	66	theme	nanoparticles	66:78	arg1	characterization					13:28	characterization	13:28	characterization	13:28	Preparation, characterization and biological evaluation of silver nanoparticles and drug loaded composites for wound dressings formed from Lallemantia royleana seeds' mucilage.
34651560	0	66	theme	nanoparticles	66:78	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, characterization and biological evaluation of silver nanoparticles and drug loaded composites for wound dressings formed from Lallemantia royleana seeds' mucilage.
34651560	0	66	theme	nanoparticles	66:78	arg1	evaluation					45:54	biological evaluation	34:54	biological evaluation	34:54	Preparation, characterization and biological evaluation of silver nanoparticles and drug loaded composites for wound dressings formed from Lallemantia royleana seeds' mucilage.
34651560	9	67	theme	drug	1217:1220	arg1	particles					1222:1230	drug particles	1217:1230	drug particles	1217:1230	The dressings were found to be porous and the silver nanoparticles and drug particles were found to be uniformly distributed in the polymeric matrix.
34651560	2	68	theme	healing	336:342	arg1	process					344:350	the healing process	332:350	the healing process	332:350	Lack of absorptive potential and sticking of dressing with the wound causes pain and slows the healing process.
34651560	12	69	theme	inhabitation	1743:1754	arg1	11-17 mm					1756:1763	inhabitation 11-17 mm	1743:1763	inhabitation 11-17 mm	1743:1763	Silver-ciprofloxacin nano-composites exhibited comparable activity (zone of inhibition 19-30 mm) against E. coli to that of ciprofloxacin (standard, 21-35 mm) and relatively lower activity in case of S. aureus (zone of inhabitation 11-17 mm).
31970866	12	0	theme	extracellular	1495:1507	arg1	%					1493:1493	18% extracellular	1491:1507	18% extracellular	1491:1507	Enrichment from human serum recovers 18% extracellular and 72% secreted glycoproteins via bottom-up approach and online tools.
31970866	10	1	theme	protein	1239:1245	arg1	coverage					1204:1211	41% sequence coverage	1191:1211	41% sequence coverage	1191:1211	Moreover, protein binding capacity is determined as 213 mg/g and 41% sequence coverage of horseradish peroxidase protein with all eight glycosylation sites detected.
31970866	10	1	theme	protein	1239:1245	arg1	213 mg/g					1178:1185	213 mg/g	1178:1185	213 mg/g	1178:1185	Moreover, protein binding capacity is determined as 213 mg/g and 41% sequence coverage of horseradish peroxidase protein with all eight glycosylation sites detected.
31970866	10	1	theme	protein	1239:1245	arg1	capacity					1152:1159	protein binding capacity	1136:1159	protein binding capacity	1136:1159	Moreover, protein binding capacity is determined as 213 mg/g and 41% sequence coverage of horseradish peroxidase protein with all eight glycosylation sites detected.
31970866	10	2	theme	horseradish	1216:1226	arg1	peroxidase					1228:1237	horseradish peroxidase	1216:1237	horseradish peroxidase protein with all eight glycosylation sites detected	1216:1289	Moreover, protein binding capacity is determined as 213 mg/g and 41% sequence coverage of horseradish peroxidase protein with all eight glycosylation sites detected.
31970866	10	3	gly	glycosylation	1262:1274	arg2	sites					1276:1280	all eight glycosylation sites	1252:1280	all eight glycosylation sites detected	1252:1289	Moreover, protein binding capacity is determined as 213 mg/g and 41% sequence coverage of horseradish peroxidase protein with all eight glycosylation sites detected.
31970866	10	3	gly	glycosylation	1262:1274	arg2	eight					1256:1260	eight	1256:1260	eight	1256:1260	Moreover, protein binding capacity is determined as 213 mg/g and 41% sequence coverage of horseradish peroxidase protein with all eight glycosylation sites detected.
31970866	12	4	theme	human	1470:1474	arg1	serum					1476:1480	human serum	1470:1480	human serum	1470:1480	Enrichment from human serum recovers 18% extracellular and 72% secreted glycoproteins via bottom-up approach and online tools.
31970866	2	5	dep	glycosylation	262:274	arg1	the					245:247	the	245:247	the	245:247	Study of glycoproteins is complex because of the simultaneous glycosylation and deglycosylation inside the body.
31970866	9	6	theme	albumin	1042:1048	arg1	digest					1050:1055	bovine serum albumin digest	1029:1055	bovine serum albumin digest	1029:1055	Boronic acid@fibrous cellulose is selective up to 1:250 for spiked horseradish peroxidase in bovine serum albumin digest, sensitive down to 0.1 femtomol and recovering 88.15% glycopeptides.
31970866	4	7	from	structure	503:511	arg1	variants					553:560	different variants	543:560	different variants	543:560	Cellulose, a natural polysaccharide, has rich surface chemistry, stable structure, low cost and availability in different variants.
31970866	1	8	theme	biological	180:189	arg1	systems					191:197	biological systems	180:197	biological systems	180:197	Enrichment of glycoproteins has been important because of their dynamicity and role in biological systems.
31970866	11	9	theme	acid	1385:1388	arg1	cellulose					1398:1406	boronic acid@fibrous cellulose	1377:1406	boronic acid@fibrous cellulose	1377:1406	Total of 18 glycopeptides are enriched from immunoglobulin digest showing ability of boronic acid@fibrous cellulose to enrich glycoproteins from multiglycoforms.
31970866	12	10	dep	%	1493:1493	arg1	glycoproteins					1526:1538	secreted glycoproteins	1517:1538	secreted glycoproteins	1517:1538	Enrichment from human serum recovers 18% extracellular and 72% secreted glycoproteins via bottom-up approach and online tools.
31970866	11	11	gly	glycopeptides	1304:1316	arg2	glycopeptides					1304:1316	18 glycopeptides	1301:1316	18 glycopeptides	1301:1316	Total of 18 glycopeptides are enriched from immunoglobulin digest showing ability of boronic acid@fibrous cellulose to enrich glycoproteins from multiglycoforms.
31970866	9	12	theme	bovine	1029:1034	arg1	albumin					1042:1048	bovine serum albumin	1029:1048	bovine serum albumin digest	1029:1055	Boronic acid@fibrous cellulose is selective up to 1:250 for spiked horseradish peroxidase in bovine serum albumin digest, sensitive down to 0.1 femtomol and recovering 88.15% glycopeptides.
31970866	4	13	theme	surface	477:483	arg1	chemistry					485:493	rich surface chemistry	472:493	rich surface chemistry	472:493	Cellulose, a natural polysaccharide, has rich surface chemistry, stable structure, low cost and availability in different variants.
31970866	11	14	theme	fibrous	1390:1396	arg1	cellulose					1398:1406	boronic acid@fibrous cellulose	1377:1406	boronic acid@fibrous cellulose	1377:1406	Total of 18 glycopeptides are enriched from immunoglobulin digest showing ability of boronic acid@fibrous cellulose to enrich glycoproteins from multiglycoforms.
31970866	10	15	theme	glycosylation	1262:1274	arg1	sites					1276:1280	all eight glycosylation sites	1252:1280	all eight glycosylation sites detected	1252:1289	Moreover, protein binding capacity is determined as 213 mg/g and 41% sequence coverage of horseradish peroxidase protein with all eight glycosylation sites detected.
31970866	3	16	from	hydrazide	361:369	arg1	materials					420:428	support materials	412:428	support materials	412:428	Often employed affinities for glycopeptides are hydrazide, boronic acid, or physiosorbed lectin on support materials.
31970866	6	17	theme	boronic	670:676	arg1	acid					678:681	boronic acid	670:681	boronic acid	670:681	Attachment of boronic acid is confirmed by Fourier transform infrared spectroscopy.
31970866	9	18	theme	serum	1036:1040	arg1	albumin					1042:1048	bovine serum albumin	1029:1048	bovine serum albumin digest	1029:1055	Boronic acid@fibrous cellulose is selective up to 1:250 for spiked horseradish peroxidase in bovine serum albumin digest, sensitive down to 0.1 femtomol and recovering 88.15% glycopeptides.
31970866	10	19	theme	peroxidase	1228:1237	arg1	protein					1239:1245	horseradish peroxidase protein	1216:1245	horseradish peroxidase protein with all eight glycosylation sites detected	1216:1289	Moreover, protein binding capacity is determined as 213 mg/g and 41% sequence coverage of horseradish peroxidase protein with all eight glycosylation sites detected.
31970866	2	20	theme	simultaneous	249:260	arg1	glycosylation					262:274	simultaneous glycosylation	249:274	simultaneous glycosylation	249:274	Study of glycoproteins is complex because of the simultaneous glycosylation and deglycosylation inside the body.
31970866	4	21	theme	low	514:516	arg1	cost					518:521	low cost	514:521	low cost	514:521	Cellulose, a natural polysaccharide, has rich surface chemistry, stable structure, low cost and availability in different variants.
31970866	0	22	theme	glycopeptides	78:90	arg1	enrichment					64:73	the selective enrichment	50:73	the selective enrichment of glycopeptides	50:90	Boronic acid functionalized fibrous cellulose for the selective enrichment of glycopeptides.
31970866	10	23	theme	protein	1136:1142	arg1	coverage					1204:1211	41% sequence coverage	1191:1211	41% sequence coverage	1191:1211	Moreover, protein binding capacity is determined as 213 mg/g and 41% sequence coverage of horseradish peroxidase protein with all eight glycosylation sites detected.
31970866	10	23	theme	protein	1136:1142	arg1	213 mg/g					1178:1185	213 mg/g	1178:1185	213 mg/g	1178:1185	Moreover, protein binding capacity is determined as 213 mg/g and 41% sequence coverage of horseradish peroxidase protein with all eight glycosylation sites detected.
31970866	10	23	theme	protein	1136:1142	arg1	capacity					1152:1159	protein binding capacity	1136:1159	protein binding capacity	1136:1159	Moreover, protein binding capacity is determined as 213 mg/g and 41% sequence coverage of horseradish peroxidase protein with all eight glycosylation sites detected.
31970866	3	24	theme	boronic	372:378	arg1	acid					380:383	boronic acid	372:383	boronic acid	372:383	Often employed affinities for glycopeptides are hydrazide, boronic acid, or physiosorbed lectin on support materials.
31970866	9	25	from	peroxidase	1015:1024	arg1	digest					1050:1055	bovine serum albumin digest	1029:1055	bovine serum albumin digest	1029:1055	Boronic acid@fibrous cellulose is selective up to 1:250 for spiked horseradish peroxidase in bovine serum albumin digest, sensitive down to 0.1 femtomol and recovering 88.15% glycopeptides.
31970866	9	26	theme	88.15	1104:1108	arg1	%					1109:1109	%	1109:1109	%	1109:1109	Boronic acid@fibrous cellulose is selective up to 1:250 for spiked horseradish peroxidase in bovine serum albumin digest, sensitive down to 0.1 femtomol and recovering 88.15% glycopeptides.
31970866	1	27	gly	glycoproteins	107:119	arg1	glycoproteins					107:119	glycoproteins	107:119	glycoproteins	107:119	Enrichment of glycoproteins has been important because of their dynamicity and role in biological systems.
31970866	5	28	theme	fibrous	581:587	arg1	cellulose					589:597	fibrous cellulose	581:597	fibrous cellulose	581:597	In present study, fibrous cellulose is oxidized using periodate to modify with boronic acid.
31970866	8	29	theme	model	921:925	arg1	protein					927:933	model protein	921:933	model protein	921:933	The enrichment efficiency is evaluated by using horseradish peroxidase as model protein.
31970866	7	30	theme	scanning	817:824	arg1	microscopy					835:844	scanning electron microscopy	817:844	scanning electron microscopy	817:844	Particle size and morphology of boronic acid@fibrous cellulose is studied by scanning electron microscopy.
31970866	6	31	dep	transform	707:715	arg1	infrared					717:724	infrared	717:724	transform infrared spectroscopy	707:737	Attachment of boronic acid is confirmed by Fourier transform infrared spectroscopy.
31970866	0	32	theme	Boronic	0:6	arg1	acid					8:11	Boronic acid	0:11	Boronic acid	0:11	Boronic acid functionalized fibrous cellulose for the selective enrichment of glycopeptides.
31970866	7	33	theme	electron	826:833	arg1	microscopy					835:844	scanning electron microscopy	817:844	scanning electron microscopy	817:844	Particle size and morphology of boronic acid@fibrous cellulose is studied by scanning electron microscopy.
31970866	11	34	gly	glycoproteins	1418:1430	arg1	glycoproteins					1418:1430	glycoproteins	1418:1430	glycoproteins from multiglycoforms	1418:1451	Total of 18 glycopeptides are enriched from immunoglobulin digest showing ability of boronic acid@fibrous cellulose to enrich glycoproteins from multiglycoforms.
31970866	3	35	theme	physiosorbed	389:400	arg1	lectin					402:407	physiosorbed lectin	389:407	physiosorbed lectin	389:407	Often employed affinities for glycopeptides are hydrazide, boronic acid, or physiosorbed lectin on support materials.
31970866	4	36	contain	has	468:470	arg2	structure					503:511	stable structure	496:511	stable structure	496:511	Cellulose, a natural polysaccharide, has rich surface chemistry, stable structure, low cost and availability in different variants.
31970866	4	36	contain	has	468:470	arg2	chemistry					485:493	rich surface chemistry	472:493	rich surface chemistry	472:493	Cellulose, a natural polysaccharide, has rich surface chemistry, stable structure, low cost and availability in different variants.
31970866	4	36	contain	has	468:470	arg2	cost					518:521	low cost	514:521	low cost	514:521	Cellulose, a natural polysaccharide, has rich surface chemistry, stable structure, low cost and availability in different variants.
31970866	4	36	contain	has	468:470	arg2	availability					527:538	availability	527:538	availability	527:538	Cellulose, a natural polysaccharide, has rich surface chemistry, stable structure, low cost and availability in different variants.
31970866	4	36	contain	has	468:470	arg1	polysaccharide					452:465	a natural polysaccharide	442:465	a natural polysaccharide	442:465	Cellulose, a natural polysaccharide, has rich surface chemistry, stable structure, low cost and availability in different variants.
31970866	4	36	contain	has	468:470	arg1	Cellulose					431:439	Cellulose	431:439	Cellulose	431:439	Cellulose, a natural polysaccharide, has rich surface chemistry, stable structure, low cost and availability in different variants.
31970866	10	37	theme	41	1191:1192	arg1	%					1193:1193	%	1193:1193	%	1193:1193	Moreover, protein binding capacity is determined as 213 mg/g and 41% sequence coverage of horseradish peroxidase protein with all eight glycosylation sites detected.
31970866	7	38	theme	fibrous	785:791	arg1	cellulose					793:801	boronic acid@fibrous cellulose	772:801	boronic acid@fibrous cellulose	772:801	Particle size and morphology of boronic acid@fibrous cellulose is studied by scanning electron microscopy.
31970866	9	39	dep	1:250	986:990	arg1	up					980:981	up	980:981	up	980:981	Boronic acid@fibrous cellulose is selective up to 1:250 for spiked horseradish peroxidase in bovine serum albumin digest, sensitive down to 0.1 femtomol and recovering 88.15% glycopeptides.
31970866	5	40	theme	boronic	642:648	arg1	acid					650:653	boronic acid	642:653	boronic acid	642:653	In present study, fibrous cellulose is oxidized using periodate to modify with boronic acid.
31970866	9	41	theme	Boronic	936:942	arg1	cellulose					957:965	Boronic acid@fibrous cellulose	936:965	Boronic acid@fibrous cellulose	936:965	Boronic acid@fibrous cellulose is selective up to 1:250 for spiked horseradish peroxidase in bovine serum albumin digest, sensitive down to 0.1 femtomol and recovering 88.15% glycopeptides.
31970866	2	42	theme	glycoproteins	209:221	arg1	Study					200:204	Study	200:204	Study of glycoproteins	200:221	Study of glycoproteins is complex because of the simultaneous glycosylation and deglycosylation inside the body.
31970866	7	43	theme	acid	780:783	arg1	cellulose					793:801	boronic acid@fibrous cellulose	772:801	boronic acid@fibrous cellulose	772:801	Particle size and morphology of boronic acid@fibrous cellulose is studied by scanning electron microscopy.
31970866	3	44	gly	glycopeptides	343:355	arg2	glycopeptides					343:355	glycopeptides	343:355	glycopeptides	343:355	Often employed affinities for glycopeptides are hydrazide, boronic acid, or physiosorbed lectin on support materials.
31970866	12	45	theme	bottom-up	1544:1552	arg1	approach					1554:1561	bottom-up approach	1544:1561	bottom-up approach	1544:1561	Enrichment from human serum recovers 18% extracellular and 72% secreted glycoproteins via bottom-up approach and online tools.
31970866	9	46	theme	@	948:948	arg1	cellulose					957:965	Boronic acid@fibrous cellulose	936:965	Boronic acid@fibrous cellulose	936:965	Boronic acid@fibrous cellulose is selective up to 1:250 for spiked horseradish peroxidase in bovine serum albumin digest, sensitive down to 0.1 femtomol and recovering 88.15% glycopeptides.
31970866	10	47	theme	sequence	1195:1202	arg1	coverage					1204:1211	41% sequence coverage	1191:1211	41% sequence coverage	1191:1211	Moreover, protein binding capacity is determined as 213 mg/g and 41% sequence coverage of horseradish peroxidase protein with all eight glycosylation sites detected.
31970866	10	47	theme	sequence	1195:1202	arg1	capacity					1152:1159	protein binding capacity	1136:1159	protein binding capacity	1136:1159	Moreover, protein binding capacity is determined as 213 mg/g and 41% sequence coverage of horseradish peroxidase protein with all eight glycosylation sites detected.
31970866	4	48	from	availability	527:538	arg1	variants					553:560	different variants	543:560	different variants	543:560	Cellulose, a natural polysaccharide, has rich surface chemistry, stable structure, low cost and availability in different variants.
31970866	4	49	from	cost	518:521	arg1	variants					553:560	different variants	543:560	different variants	543:560	Cellulose, a natural polysaccharide, has rich surface chemistry, stable structure, low cost and availability in different variants.
31970866	7	50	theme	Particle	740:747	arg1	size					749:752	Particle size	740:752	Particle size	740:752	Particle size and morphology of boronic acid@fibrous cellulose is studied by scanning electron microscopy.
31970866	12	51	from	serum	1476:1480	arg1	Enrichment					1454:1463	Enrichment	1454:1463	Enrichment from human serum	1454:1480	Enrichment from human serum recovers 18% extracellular and 72% secreted glycoproteins via bottom-up approach and online tools.
31970866	9	52	theme	spiked	996:1001	arg1	peroxidase					1015:1024	spiked horseradish peroxidase	996:1024	spiked horseradish peroxidase in bovine serum albumin digest	996:1055	Boronic acid@fibrous cellulose is selective up to 1:250 for spiked horseradish peroxidase in bovine serum albumin digest, sensitive down to 0.1 femtomol and recovering 88.15% glycopeptides.
31970866	11	53	theme	boronic	1377:1383	arg1	cellulose					1398:1406	boronic acid@fibrous cellulose	1377:1406	boronic acid@fibrous cellulose	1377:1406	Total of 18 glycopeptides are enriched from immunoglobulin digest showing ability of boronic acid@fibrous cellulose to enrich glycoproteins from multiglycoforms.
31970866	9	54	theme	horseradish	1003:1013	arg1	peroxidase					1015:1024	spiked horseradish peroxidase	996:1024	spiked horseradish peroxidase in bovine serum albumin digest	996:1055	Boronic acid@fibrous cellulose is selective up to 1:250 for spiked horseradish peroxidase in bovine serum albumin digest, sensitive down to 0.1 femtomol and recovering 88.15% glycopeptides.
31970866	4	55	from	chemistry	485:493	arg1	variants					553:560	different variants	543:560	different variants	543:560	Cellulose, a natural polysaccharide, has rich surface chemistry, stable structure, low cost and availability in different variants.
31970866	4	56	theme	rich	472:475	arg1	chemistry					485:493	rich surface chemistry	472:493	rich surface chemistry	472:493	Cellulose, a natural polysaccharide, has rich surface chemistry, stable structure, low cost and availability in different variants.
31970866	11	57	theme	cellulose	1398:1406	arg1	ability					1366:1372	ability	1366:1372	ability of boronic acid@fibrous cellulose to enrich glycoproteins from multiglycoforms	1366:1451	Total of 18 glycopeptides are enriched from immunoglobulin digest showing ability of boronic acid@fibrous cellulose to enrich glycoproteins from multiglycoforms.
31970866	3	58	from	acid	380:383	arg1	materials					420:428	support materials	412:428	support materials	412:428	Often employed affinities for glycopeptides are hydrazide, boronic acid, or physiosorbed lectin on support materials.
31970866	11	59	theme	@	1389:1389	arg1	cellulose					1398:1406	boronic acid@fibrous cellulose	1377:1406	boronic acid@fibrous cellulose	1377:1406	Total of 18 glycopeptides are enriched from immunoglobulin digest showing ability of boronic acid@fibrous cellulose to enrich glycoproteins from multiglycoforms.
31970866	5	60	theme	present	566:572	arg1	study					574:578	present study	566:578	present study	566:578	In present study, fibrous cellulose is oxidized using periodate to modify with boronic acid.
31970866	4	61	theme	natural	444:450	arg1	polysaccharide					452:465	a natural polysaccharide	442:465	a natural polysaccharide	442:465	Cellulose, a natural polysaccharide, has rich surface chemistry, stable structure, low cost and availability in different variants.
31970866	4	61	theme	natural	444:450	arg1	Cellulose					431:439	Cellulose	431:439	Cellulose	431:439	Cellulose, a natural polysaccharide, has rich surface chemistry, stable structure, low cost and availability in different variants.
31970866	6	62	theme	acid	678:681	arg1	Attachment					656:665	Attachment	656:665	Attachment of boronic acid	656:681	Attachment of boronic acid is confirmed by Fourier transform infrared spectroscopy.
31970866	3	63	theme	employed	319:326	arg1	hydrazide					361:369	hydrazide	361:369	hydrazide	361:369	Often employed affinities for glycopeptides are hydrazide, boronic acid, or physiosorbed lectin on support materials.
31970866	3	63	theme	employed	319:326	arg1	affinities					328:337	Often employed affinities	313:337	Often employed affinities for glycopeptides	313:355	Often employed affinities for glycopeptides are hydrazide, boronic acid, or physiosorbed lectin on support materials.
31970866	2	64	gly	glycoproteins	209:221	arg1	glycoproteins					209:221	glycoproteins	209:221	glycoproteins	209:221	Study of glycoproteins is complex because of the simultaneous glycosylation and deglycosylation inside the body.
31970866	11	65	from	multiglycoforms	1437:1451	arg1	glycoproteins					1418:1430	glycoproteins	1418:1430	glycoproteins from multiglycoforms	1418:1451	Total of 18 glycopeptides are enriched from immunoglobulin digest showing ability of boronic acid@fibrous cellulose to enrich glycoproteins from multiglycoforms.
31970866	8	66	theme	enrichment	851:860	arg1	efficiency					862:871	The enrichment efficiency	847:871	The enrichment efficiency	847:871	The enrichment efficiency is evaluated by using horseradish peroxidase as model protein.
31970866	1	67	from	role	172:175	arg1	systems					191:197	biological systems	180:197	biological systems	180:197	Enrichment of glycoproteins has been important because of their dynamicity and role in biological systems.
31970866	4	68	theme	stable	496:501	arg1	structure					503:511	stable structure	496:511	stable structure	496:511	Cellulose, a natural polysaccharide, has rich surface chemistry, stable structure, low cost and availability in different variants.
31970866	11	69	theme	glycopeptides	1304:1316	arg1	Total					1292:1296	Total	1292:1296	Total of 18 glycopeptides	1292:1316	Total of 18 glycopeptides are enriched from immunoglobulin digest showing ability of boronic acid@fibrous cellulose to enrich glycoproteins from multiglycoforms.
31970866	6	70	dep	Fourier	699:705	arg1	transform					707:715	transform	707:715	transform infrared spectroscopy	707:737	Attachment of boronic acid is confirmed by Fourier transform infrared spectroscopy.
31970866	3	71	from	lectin	402:407	arg1	materials					420:428	support materials	412:428	support materials	412:428	Often employed affinities for glycopeptides are hydrazide, boronic acid, or physiosorbed lectin on support materials.
31970866	1	72	from	dynamicity	157:166	arg1	systems					191:197	biological systems	180:197	biological systems	180:197	Enrichment of glycoproteins has been important because of their dynamicity and role in biological systems.
31970866	0	73	gly	glycopeptides	78:90	arg2	glycopeptides					78:90	glycopeptides	78:90	glycopeptides	78:90	Boronic acid functionalized fibrous cellulose for the selective enrichment of glycopeptides.
31970866	8	74	theme	horseradish	895:905	arg1	peroxidase					907:916	horseradish peroxidase	895:916	horseradish peroxidase	895:916	The enrichment efficiency is evaluated by using horseradish peroxidase as model protein.
31970866	9	75	theme	%	1109:1109	arg1	glycopeptides					1111:1123	88.15% glycopeptides	1104:1123	88.15% glycopeptides	1104:1123	Boronic acid@fibrous cellulose is selective up to 1:250 for spiked horseradish peroxidase in bovine serum albumin digest, sensitive down to 0.1 femtomol and recovering 88.15% glycopeptides.
31970866	3	76	theme	support	412:418	arg1	materials					420:428	support materials	412:428	support materials	412:428	Often employed affinities for glycopeptides are hydrazide, boronic acid, or physiosorbed lectin on support materials.
31970866	11	77	theme	immunoglobulin	1336:1349	arg1	digest					1351:1356	immunoglobulin digest	1336:1356	immunoglobulin digest showing ability of boronic acid@fibrous cellulose to enrich glycoproteins from multiglycoforms	1336:1451	Total of 18 glycopeptides are enriched from immunoglobulin digest showing ability of boronic acid@fibrous cellulose to enrich glycoproteins from multiglycoforms.
31970866	0	78	theme	fibrous	28:34	arg1	cellulose					36:44	fibrous cellulose	28:44	fibrous cellulose	28:44	Boronic acid functionalized fibrous cellulose for the selective enrichment of glycopeptides.
31970866	1	79	theme	glycoproteins	107:119	arg1	Enrichment					93:102	Enrichment	93:102	Enrichment of glycoproteins	93:119	Enrichment of glycoproteins has been important because of their dynamicity and role in biological systems.
31970866	10	80	with	protein	1239:1245	arg1	sites					1276:1280	all eight glycosylation sites	1252:1280	all eight glycosylation sites detected	1252:1289	Moreover, protein binding capacity is determined as 213 mg/g and 41% sequence coverage of horseradish peroxidase protein with all eight glycosylation sites detected.
31970866	12	81	gly	glycoproteins	1526:1538	arg1	glycoproteins					1526:1538	secreted glycoproteins	1517:1538	secreted glycoproteins	1517:1538	Enrichment from human serum recovers 18% extracellular and 72% secreted glycoproteins via bottom-up approach and online tools.
31970866	10	82	theme	binding	1144:1150	arg1	coverage					1204:1211	41% sequence coverage	1191:1211	41% sequence coverage	1191:1211	Moreover, protein binding capacity is determined as 213 mg/g and 41% sequence coverage of horseradish peroxidase protein with all eight glycosylation sites detected.
31970866	10	82	theme	binding	1144:1150	arg1	213 mg/g					1178:1185	213 mg/g	1178:1185	213 mg/g	1178:1185	Moreover, protein binding capacity is determined as 213 mg/g and 41% sequence coverage of horseradish peroxidase protein with all eight glycosylation sites detected.
31970866	10	82	theme	binding	1144:1150	arg1	capacity					1152:1159	protein binding capacity	1136:1159	protein binding capacity	1136:1159	Moreover, protein binding capacity is determined as 213 mg/g and 41% sequence coverage of horseradish peroxidase protein with all eight glycosylation sites detected.
31970866	9	83	gly	glycopeptides	1111:1123	arg2	glycopeptides					1111:1123	88.15% glycopeptides	1104:1123	88.15% glycopeptides	1104:1123	Boronic acid@fibrous cellulose is selective up to 1:250 for spiked horseradish peroxidase in bovine serum albumin digest, sensitive down to 0.1 femtomol and recovering 88.15% glycopeptides.
31970866	4	84	theme	different	543:551	arg1	variants					553:560	different variants	543:560	different variants	543:560	Cellulose, a natural polysaccharide, has rich surface chemistry, stable structure, low cost and availability in different variants.
31970866	0	85	theme	selective	54:62	arg1	enrichment					64:73	the selective enrichment	50:73	the selective enrichment of glycopeptides	50:90	Boronic acid functionalized fibrous cellulose for the selective enrichment of glycopeptides.
31970866	12	86	theme	online	1567:1572	arg1	tools					1574:1578	online tools	1567:1578	online tools	1567:1578	Enrichment from human serum recovers 18% extracellular and 72% secreted glycoproteins via bottom-up approach and online tools.
31970866	7	87	theme	cellulose	793:801	arg1	morphology					758:767	morphology	758:767	morphology	758:767	Particle size and morphology of boronic acid@fibrous cellulose is studied by scanning electron microscopy.
31970866	7	87	theme	cellulose	793:801	arg1	size					749:752	Particle size	740:752	Particle size	740:752	Particle size and morphology of boronic acid@fibrous cellulose is studied by scanning electron microscopy.
31970866	12	88	theme	secreted	1517:1524	arg1	glycoproteins					1526:1538	secreted glycoproteins	1517:1538	secreted glycoproteins	1517:1538	Enrichment from human serum recovers 18% extracellular and 72% secreted glycoproteins via bottom-up approach and online tools.
31970866	9	89	theme	acid	944:947	arg1	cellulose					957:965	Boronic acid@fibrous cellulose	936:965	Boronic acid@fibrous cellulose	936:965	Boronic acid@fibrous cellulose is selective up to 1:250 for spiked horseradish peroxidase in bovine serum albumin digest, sensitive down to 0.1 femtomol and recovering 88.15% glycopeptides.
31970866	7	90	theme	@	784:784	arg1	cellulose					793:801	boronic acid@fibrous cellulose	772:801	boronic acid@fibrous cellulose	772:801	Particle size and morphology of boronic acid@fibrous cellulose is studied by scanning electron microscopy.
31970866	9	91	theme	fibrous	949:955	arg1	cellulose					957:965	Boronic acid@fibrous cellulose	936:965	Boronic acid@fibrous cellulose	936:965	Boronic acid@fibrous cellulose is selective up to 1:250 for spiked horseradish peroxidase in bovine serum albumin digest, sensitive down to 0.1 femtomol and recovering 88.15% glycopeptides.
31970866	7	92	theme	boronic	772:778	arg1	cellulose					793:801	boronic acid@fibrous cellulose	772:801	boronic acid@fibrous cellulose	772:801	Particle size and morphology of boronic acid@fibrous cellulose is studied by scanning electron microscopy.
31970866	10	93	theme	%	1193:1193	arg1	coverage					1204:1211	41% sequence coverage	1191:1211	41% sequence coverage	1191:1211	Moreover, protein binding capacity is determined as 213 mg/g and 41% sequence coverage of horseradish peroxidase protein with all eight glycosylation sites detected.
31970866	10	93	theme	%	1193:1193	arg1	capacity					1152:1159	protein binding capacity	1136:1159	protein binding capacity	1136:1159	Moreover, protein binding capacity is determined as 213 mg/g and 41% sequence coverage of horseradish peroxidase protein with all eight glycosylation sites detected.
32565301	8	0	located	localized	891:899	arg2	residues					878:885	Galf residues	873:885	Galf residues	873:885	Galf residues are localized as terminal units predominantly in the β configuration, while α-D-Galp and α-L-Araf may be partially O-methylated.
32565301	8	0	located	localized	891:899	arg1	configuration					942:954	the β configuration	936:954	the β configuration	936:954	Galf residues are localized as terminal units predominantly in the β configuration, while α-D-Galp and α-L-Araf may be partially O-methylated.
32565301	8	0	located	localized	891:899	arg2	units					913:917	terminal units	904:917	terminal units	904:917	Galf residues are localized as terminal units predominantly in the β configuration, while α-D-Galp and α-L-Araf may be partially O-methylated.
32565301	9	1	theme	guinea	1187:1192	arg1	pig					1194:1196	guinea pig	1187:1196	guinea pig model experimental asthma	1187:1222	Ex vivo biological assays showed increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels corresponding to transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma.
32565301	1	2	theme	great	184:188	arg1	attention					190:198	great attention	184:198	great attention	184:198	Microalgal exopolysaccharides (EPSs) are given great attention due to their potential biotechnology applications.
32565301	10	3	theme	EPS	1287:1289	arg1	effect					1267:1272	the anti-remodelling effect	1246:1272	the anti-remodelling effect of Chlorella EPS	1246:1289	These facts point to the anti-remodelling effect of Chlorella EPS and suggest its possible application in the treatment of asthma and chronic obstructive pulmonary disorder.
32565301	9	4	theme	growth	1148:1153	arg1	TGF-β					1168:1172	TGF-β	1168:1172	TGF-β	1168:1172	Ex vivo biological assays showed increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels corresponding to transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma.
32565301	9	4	theme	growth	1148:1153	arg1	beta					1162:1165	transforming growth factor beta	1135:1165	transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma	1135:1222	Ex vivo biological assays showed increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels corresponding to transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma.
32565301	0	5	theme	anti-inflammatory	89:105	arg1	effects					128:134	its anti-inflammatory and anti-remodelling effects	85:134	its anti-inflammatory and anti-remodelling effects	85:134	Chlorella vulgaris α-L-arabino-α-L-rhamno-α,β-D-galactan structure and mechanisms of its anti-inflammatory and anti-remodelling effects.
32565301	6	6	dep	α-L-Araf	766:773	arg1	residues					798:805	residues	798:805	residues	798:805	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	9	7	theme	pig	1194:1196	arg1	asthma					1217:1222	guinea pig model experimental asthma	1187:1222	guinea pig model experimental asthma	1187:1222	Ex vivo biological assays showed increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels corresponding to transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma.
32565301	6	8	theme	branched	680:687	arg1	1 → sequence					659:670	→2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence	631:670	→2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence	631:670	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	6	8	theme	branched	680:687	arg1	C2					750:751	highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2	673:751	highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2	673:751	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	10	9	theme	disorder	1389:1396	arg1	treatment					1335:1343	the treatment	1331:1343	the treatment of asthma and chronic obstructive pulmonary disorder	1331:1396	These facts point to the anti-remodelling effect of Chlorella EPS and suggest its possible application in the treatment of asthma and chronic obstructive pulmonary disorder.
32565301	0	10	theme	effects	128:134	arg1	α-L-arabino-α-L-rhamno-α					19:42	Chlorella vulgaris α-L-arabino-α-L-rhamno-α	0:42	Chlorella vulgaris α-L-arabino-α-L-rhamno-α	0:42	Chlorella vulgaris α-L-arabino-α-L-rhamno-α,β-D-galactan structure and mechanisms of its anti-inflammatory and anti-remodelling effects.
32565301	0	10	theme	effects	128:134	arg1	structure					57:65	β-D-galactan structure	44:65	β-D-galactan structure	44:65	Chlorella vulgaris α-L-arabino-α-L-rhamno-α,β-D-galactan structure and mechanisms of its anti-inflammatory and anti-remodelling effects.
32565301	0	10	theme	effects	128:134	arg1	mechanisms					71:80	mechanisms	71:80	mechanisms	71:80	Chlorella vulgaris α-L-arabino-α-L-rhamno-α,β-D-galactan structure and mechanisms of its anti-inflammatory and anti-remodelling effects.
32565301	9	11	theme	model	1198:1202	arg1	asthma					1217:1222	guinea pig model experimental asthma	1187:1222	guinea pig model experimental asthma	1187:1222	Ex vivo biological assays showed increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels corresponding to transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma.
32565301	8	12	theme	Galf	873:876	arg1	units					913:917	terminal units	904:917	terminal units	904:917	Galf residues are localized as terminal units predominantly in the β configuration, while α-D-Galp and α-L-Araf may be partially O-methylated.
32565301	8	12	theme	Galf	873:876	arg1	residues					878:885	Galf residues	873:885	Galf residues	873:885	Galf residues are localized as terminal units predominantly in the β configuration, while α-D-Galp and α-L-Araf may be partially O-methylated.
32565301	3	13	theme	oligosaccharide	418:432	arg1	fractions					434:442	oligosaccharide fractions	418:442	oligosaccharide fractions	418:442	Hydrolysate separation by gel chromatography afforded oligosaccharide fractions.
32565301	9	14	theme	beta	1162:1165	arg1	decrease					1175:1182	transforming growth factor beta (TGF-β) decrease	1135:1182	transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma	1135:1222	Ex vivo biological assays showed increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels corresponding to transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma.
32565301	9	15	theme	experimental	1204:1215	arg1	asthma					1217:1222	guinea pig model experimental asthma	1187:1222	guinea pig model experimental asthma	1187:1222	Ex vivo biological assays showed increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels corresponding to transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma.
32565301	6	16	from	C2	750:751	arg1	at					747:748	highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2	673:751	highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2	673:751	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	3	17	theme	gel	390:392	arg1	chromatography					394:407	gel chromatography	390:407	gel chromatography	390:407	Hydrolysate separation by gel chromatography afforded oligosaccharide fractions.
32565301	9	18	dep	showed	1042:1047	arg1	corresponding					1118:1130	corresponding	1118:1130	showed increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels corresponding to transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma	1042:1222	Ex vivo biological assays showed increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels corresponding to transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma.
32565301	2	19	theme	linkage	317:323	arg1	analyses					325:332	sugar linkage analyses	311:332	sugar linkage analyses	311:332	Purified C. vulgaris EPS was subjected to compositional and sugar linkage analyses, and partial acid hydrolysis.
32565301	9	20	theme	interleukin-12	1059:1072	arg1	levels					1111:1116	increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels	1049:1116	increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels	1049:1116	Ex vivo biological assays showed increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels corresponding to transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma.
32565301	6	21	theme	Backbone	594:601	arg1	unit					613:616	Backbone repeating unit	594:616	Backbone repeating unit	594:616	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	2	22	theme	sugar	311:315	arg1	analyses					325:332	sugar linkage analyses	311:332	sugar linkage analyses	311:332	Purified C. vulgaris EPS was subjected to compositional and sugar linkage analyses, and partial acid hydrolysis.
32565301	8	23	theme	terminal	904:911	arg1	units					913:917	terminal units	904:917	terminal units	904:917	Galf residues are localized as terminal units predominantly in the β configuration, while α-D-Galp and α-L-Araf may be partially O-methylated.
32565301	8	23	theme	terminal	904:911	arg1	residues					878:885	Galf residues	873:885	Galf residues	873:885	Galf residues are localized as terminal units predominantly in the β configuration, while α-D-Galp and α-L-Araf may be partially O-methylated.
32565301	6	24	theme	repeating	603:611	arg1	unit					613:616	Backbone repeating unit	594:616	Backbone repeating unit	594:616	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	0	25	theme	vulgaris	10:17	arg1	α-L-arabino-α-L-rhamno-α					19:42	Chlorella vulgaris α-L-arabino-α-L-rhamno-α	0:42	Chlorella vulgaris α-L-arabino-α-L-rhamno-α	0:42	Chlorella vulgaris α-L-arabino-α-L-rhamno-α,β-D-galactan structure and mechanisms of its anti-inflammatory and anti-remodelling effects.
32565301	0	25	theme	vulgaris	10:17	arg1	structure					57:65	β-D-galactan structure	44:65	β-D-galactan structure	44:65	Chlorella vulgaris α-L-arabino-α-L-rhamno-α,β-D-galactan structure and mechanisms of its anti-inflammatory and anti-remodelling effects.
32565301	0	25	theme	vulgaris	10:17	arg1	mechanisms					71:80	mechanisms	71:80	mechanisms	71:80	Chlorella vulgaris α-L-arabino-α-L-rhamno-α,β-D-galactan structure and mechanisms of its anti-inflammatory and anti-remodelling effects.
32565301	3	26	theme	Hydrolysate	364:374	arg1	separation					376:385	Hydrolysate separation	364:385	Hydrolysate separation by gel chromatography	364:407	Hydrolysate separation by gel chromatography afforded oligosaccharide fractions.
32565301	9	27	dep	Ex	1016:1017	arg1	vivo					1019:1022	vivo	1019:1022	vivo	1019:1022	Ex vivo biological assays showed increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels corresponding to transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma.
32565301	0	28	theme	Chlorella	0:8	arg1	α-L-arabino-α-L-rhamno-α					19:42	Chlorella vulgaris α-L-arabino-α-L-rhamno-α	0:42	Chlorella vulgaris α-L-arabino-α-L-rhamno-α	0:42	Chlorella vulgaris α-L-arabino-α-L-rhamno-α,β-D-galactan structure and mechanisms of its anti-inflammatory and anti-remodelling effects.
32565301	0	28	theme	Chlorella	0:8	arg1	structure					57:65	β-D-galactan structure	44:65	β-D-galactan structure	44:65	Chlorella vulgaris α-L-arabino-α-L-rhamno-α,β-D-galactan structure and mechanisms of its anti-inflammatory and anti-remodelling effects.
32565301	0	28	theme	Chlorella	0:8	arg1	mechanisms					71:80	mechanisms	71:80	mechanisms	71:80	Chlorella vulgaris α-L-arabino-α-L-rhamno-α,β-D-galactan structure and mechanisms of its anti-inflammatory and anti-remodelling effects.
32565301	10	29	theme	pulmonary	1379:1387	arg1	disorder					1389:1396	chronic obstructive pulmonary disorder	1359:1396	chronic obstructive pulmonary disorder	1359:1396	These facts point to the anti-remodelling effect of Chlorella EPS and suggest its possible application in the treatment of asthma and chronic obstructive pulmonary disorder.
32565301	2	30	theme	acid	347:350	arg1	hydrolysis					352:361	partial acid hydrolysis	339:361	partial acid hydrolysis	339:361	Purified C. vulgaris EPS was subjected to compositional and sugar linkage analyses, and partial acid hydrolysis.
32565301	5	31	theme	complex	521:527	arg1	structure					583:591	very complex highly branched α-L-arabino-α-L-rhamno-α,β-D-galactan structure	516:591	very complex highly branched α-L-arabino-α-L-rhamno-α,β-D-galactan structure	516:591	Data suggest very complex highly branched α-L-arabino-α-L-rhamno-α,β-D-galactan structure.
32565301	1	32	theme	potential	213:221	arg1	applications					237:248	their potential biotechnology applications	207:248	their potential biotechnology applications	207:248	Microalgal exopolysaccharides (EPSs) are given great attention due to their potential biotechnology applications.
32565301	10	33	theme	anti-remodelling	1250:1265	arg1	effect					1267:1272	the anti-remodelling effect	1246:1272	the anti-remodelling effect of Chlorella EPS	1246:1289	These facts point to the anti-remodelling effect of Chlorella EPS and suggest its possible application in the treatment of asthma and chronic obstructive pulmonary disorder.
32565301	10	34	theme	possible	1307:1314	arg1	application					1316:1326	its possible application	1303:1326	its possible application in the treatment of asthma and chronic obstructive pulmonary disorder	1303:1396	These facts point to the anti-remodelling effect of Chlorella EPS and suggest its possible application in the treatment of asthma and chronic obstructive pulmonary disorder.
32565301	10	35	theme	obstructive	1367:1377	arg1	disorder					1389:1396	chronic obstructive pulmonary disorder	1359:1396	chronic obstructive pulmonary disorder	1359:1396	These facts point to the anti-remodelling effect of Chlorella EPS and suggest its possible application in the treatment of asthma and chronic obstructive pulmonary disorder.
32565301	2	36	theme	partial	339:345	arg1	hydrolysis					352:361	partial acid hydrolysis	339:361	partial acid hydrolysis	339:361	Purified C. vulgaris EPS was subjected to compositional and sugar linkage analyses, and partial acid hydrolysis.
32565301	1	37	theme	biotechnology	223:235	arg1	applications					237:248	their potential biotechnology applications	207:248	their potential biotechnology applications	207:248	Microalgal exopolysaccharides (EPSs) are given great attention due to their potential biotechnology applications.
32565301	6	38	theme	-α-L-Rha	650:657	arg1	C3					754:755	C3	754:755	C3	754:755	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	6	38	theme	-α-L-Rha	650:657	arg1	C4					760:761	C4	760:761	C4	760:761	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	6	38	theme	-α-L-Rha	650:657	arg1	1 → sequence					659:670	→2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence	631:670	→2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence	631:670	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	6	38	theme	-α-L-Rha	650:657	arg1	C2					750:751	highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2	673:751	highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2	673:751	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	7	39	theme	1,2-linked	829:838	arg1	chains					840:845	longer 1,2-linked chains	822:845	longer 1,2-linked chains	822:845	α-L-Araf form longer 1,2-linked chains branched at C3, C4 or C5.
32565301	6	40	link	1,6-linked	697:706	arg1	chains					722:727	long 1,6-linked α-D-Galp side chains	692:727	long 1,6-linked α-D-Galp side chains	692:727	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	1	41	theme	Microalgal	137:146	arg1	EPSs					168:171	EPSs	168:171	EPSs	168:171	Microalgal exopolysaccharides (EPSs) are given great attention due to their potential biotechnology applications.
32565301	1	41	theme	Microalgal	137:146	arg1	exopolysaccharides					148:165	Microalgal exopolysaccharides	137:165	Microalgal exopolysaccharides (EPSs)	137:172	Microalgal exopolysaccharides (EPSs) are given great attention due to their potential biotechnology applications.
32565301	9	42	theme	transforming	1135:1146	arg1	TGF-β					1168:1172	TGF-β	1168:1172	TGF-β	1168:1172	Ex vivo biological assays showed increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels corresponding to transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma.
32565301	9	42	theme	transforming	1135:1146	arg1	beta					1162:1165	transforming growth factor beta	1135:1165	transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma	1135:1222	Ex vivo biological assays showed increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels corresponding to transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma.
32565301	6	43	theme	at	747:748	arg1	1 → sequence					659:670	→2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence	631:670	→2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence	631:670	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	6	43	theme	at	747:748	arg1	C2					750:751	highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2	673:751	highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2	673:751	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	9	44	theme	Ex	1016:1017	arg1	assays					1035:1040	Ex vivo biological assays	1016:1040	Ex vivo biological assays	1016:1040	Ex vivo biological assays showed increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels corresponding to transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma.
32565301	9	45	theme	increased	1049:1057	arg1	levels					1111:1116	increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels	1049:1116	increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels	1049:1116	Ex vivo biological assays showed increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels corresponding to transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma.
32565301	10	46	theme	Chlorella	1277:1285	arg1	EPS					1287:1289	Chlorella EPS	1277:1289	Chlorella EPS	1277:1289	These facts point to the anti-remodelling effect of Chlorella EPS and suggest its possible application in the treatment of asthma and chronic obstructive pulmonary disorder.
32565301	5	47	theme	α-L-arabino-α-L-rhamno-α	545:568	arg1	structure					583:591	very complex highly branched α-L-arabino-α-L-rhamno-α,β-D-galactan structure	516:591	very complex highly branched α-L-arabino-α-L-rhamno-α,β-D-galactan structure	516:591	Data suggest very complex highly branched α-L-arabino-α-L-rhamno-α,β-D-galactan structure.
32565301	0	48	theme	β-D-galactan	44:55	arg1	α-L-arabino-α-L-rhamno-α					19:42	Chlorella vulgaris α-L-arabino-α-L-rhamno-α	0:42	Chlorella vulgaris α-L-arabino-α-L-rhamno-α	0:42	Chlorella vulgaris α-L-arabino-α-L-rhamno-α,β-D-galactan structure and mechanisms of its anti-inflammatory and anti-remodelling effects.
32565301	0	48	theme	β-D-galactan	44:55	arg1	structure					57:65	β-D-galactan structure	44:65	β-D-galactan structure	44:65	Chlorella vulgaris α-L-arabino-α-L-rhamno-α,β-D-galactan structure and mechanisms of its anti-inflammatory and anti-remodelling effects.
32565301	6	49	theme	1 → 3	644:648	arg1	C3					754:755	C3	754:755	C3	754:755	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	6	49	theme	1 → 3	644:648	arg1	C4					760:761	C4	760:761	C4	760:761	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	6	49	theme	1 → 3	644:648	arg1	1 → sequence					659:670	→2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence	631:670	→2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence	631:670	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	6	49	theme	1 → 3	644:648	arg1	C2					750:751	highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2	673:751	highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2	673:751	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	6	50	dep	branched	680:687	arg1	branched					738:745	branched	738:745	branched	738:745	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	9	51	theme	biological	1024:1033	arg1	assays					1035:1040	Ex vivo biological assays	1016:1040	Ex vivo biological assays	1016:1040	Ex vivo biological assays showed increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels corresponding to transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma.
32565301	7	52	theme	longer	822:827	arg1	chains					840:845	longer 1,2-linked chains	822:845	longer 1,2-linked chains	822:845	α-L-Araf form longer 1,2-linked chains branched at C3, C4 or C5.
32565301	2	53	theme	vulgaris	263:270	arg1	EPS					272:274	Purified C. vulgaris EPS	251:274	Purified C. vulgaris EPS	251:274	Purified C. vulgaris EPS was subjected to compositional and sugar linkage analyses, and partial acid hydrolysis.
32565301	8	54	theme	β	940:940	arg1	configuration					942:954	the β configuration	936:954	the β configuration	936:954	Galf residues are localized as terminal units predominantly in the β configuration, while α-D-Galp and α-L-Araf may be partially O-methylated.
32565301	10	55	theme	chronic	1359:1365	arg1	disorder					1389:1396	chronic obstructive pulmonary disorder	1359:1396	chronic obstructive pulmonary disorder	1359:1396	These facts point to the anti-remodelling effect of Chlorella EPS and suggest its possible application in the treatment of asthma and chronic obstructive pulmonary disorder.
32565301	6	56	theme	-α-L-Rha	634:641	arg1	C3					754:755	C3	754:755	C3	754:755	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	6	56	theme	-α-L-Rha	634:641	arg1	C4					760:761	C4	760:761	C4	760:761	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	6	56	theme	-α-L-Rha	634:641	arg1	1 → sequence					659:670	→2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence	631:670	→2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence	631:670	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	6	56	theme	-α-L-Rha	634:641	arg1	C2					750:751	highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2	673:751	highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2	673:751	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	2	57	theme	Purified	251:258	arg1	EPS					272:274	Purified C. vulgaris EPS	251:274	Purified C. vulgaris EPS	251:274	Purified C. vulgaris EPS was subjected to compositional and sugar linkage analyses, and partial acid hydrolysis.
32565301	2	58	theme	C.	260:261	arg1	EPS					272:274	Purified C. vulgaris EPS	251:274	Purified C. vulgaris EPS	251:274	Purified C. vulgaris EPS was subjected to compositional and sugar linkage analyses, and partial acid hydrolysis.
32565301	9	59	from	decrease	1175:1182	arg1	asthma					1217:1222	guinea pig model experimental asthma	1187:1222	guinea pig model experimental asthma	1187:1222	Ex vivo biological assays showed increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels corresponding to transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma.
32565301	9	60	theme	interferon-gamma	1086:1101	arg1	levels					1111:1116	increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels	1049:1116	increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels	1049:1116	Ex vivo biological assays showed increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels corresponding to transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma.
32565301	10	61	theme	asthma	1348:1353	arg1	treatment					1335:1343	the treatment	1331:1343	the treatment of asthma and chronic obstructive pulmonary disorder	1331:1396	These facts point to the anti-remodelling effect of Chlorella EPS and suggest its possible application in the treatment of asthma and chronic obstructive pulmonary disorder.
32565301	6	62	theme	side	717:720	arg1	chains					722:727	long 1,6-linked α-D-Galp side chains	692:727	long 1,6-linked α-D-Galp side chains	692:727	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	9	63	theme	factor	1155:1160	arg1	TGF-β					1168:1172	TGF-β	1168:1172	TGF-β	1168:1172	Ex vivo biological assays showed increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels corresponding to transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma.
32565301	9	63	theme	factor	1155:1160	arg1	beta					1162:1165	transforming growth factor beta	1135:1165	transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma	1135:1222	Ex vivo biological assays showed increased interleukin-12 (IL-12) and interferon-gamma (INF-γ) levels corresponding to transforming growth factor beta (TGF-β) decrease in guinea pig model experimental asthma.
32565301	7	64	link	1,2-linked	829:838	arg1	chains					840:845	longer 1,2-linked chains	822:845	longer 1,2-linked chains	822:845	α-L-Araf form longer 1,2-linked chains branched at C3, C4 or C5.
32565301	6	65	theme	α-D-Galp	708:715	arg1	chains					722:727	long 1,6-linked α-D-Galp side chains	692:727	long 1,6-linked α-D-Galp side chains	692:727	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	4	66	theme	NMR	485:487	arg1	spectroscopy					489:500	NMR spectroscopy	485:500	NMR spectroscopy	485:500	Both, EPS and oligomers were studied by NMR spectroscopy.
32565301	5	67	dep	α-L-arabino-α-L-rhamno-α	545:568	arg1	β-D-galactan					570:581	β-D-galactan	570:581	β-D-galactan	570:581	Data suggest very complex highly branched α-L-arabino-α-L-rhamno-α,β-D-galactan structure.
32565301	6	68	theme	1,6-linked	697:706	arg1	chains					722:727	long 1,6-linked α-D-Galp side chains	692:727	long 1,6-linked α-D-Galp side chains	692:727	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	6	69	theme	→2	631:632	arg1	C3					754:755	C3	754:755	C3	754:755	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	6	69	theme	→2	631:632	arg1	C4					760:761	C4	760:761	C4	760:761	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	6	69	theme	→2	631:632	arg1	1 → sequence					659:670	→2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence	631:670	→2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence	631:670	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	6	69	theme	→2	631:632	arg1	C2					750:751	highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2	673:751	highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2	673:751	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	5	70	dep	complex	521:527	arg1	branched					536:543	branched	536:543	branched	536:543	Data suggest very complex highly branched α-L-arabino-α-L-rhamno-α,β-D-galactan structure.
32565301	10	71	from	application	1316:1326	arg1	treatment					1335:1343	the treatment	1331:1343	the treatment of asthma and chronic obstructive pulmonary disorder	1331:1396	These facts point to the anti-remodelling effect of Chlorella EPS and suggest its possible application in the treatment of asthma and chronic obstructive pulmonary disorder.
32565301	6	72	theme	long	692:695	arg1	chains					722:727	long 1,6-linked α-D-Galp side chains	692:727	long 1,6-linked α-D-Galp side chains	692:727	Backbone repeating unit is formed by →2)-α-L-Rha (1 → 3)-α-L-Rha(1 → sequence, highly branched by long 1,6-linked α-D-Galp side chains, further branched at C2, C3 or C4 by α-L-Araf, α-D-Galf and β-D-Galf residues.
32565301	0	73	theme	anti-remodelling	111:126	arg1	effects					128:134	its anti-inflammatory and anti-remodelling effects	85:134	its anti-inflammatory and anti-remodelling effects	85:134	Chlorella vulgaris α-L-arabino-α-L-rhamno-α,β-D-galactan structure and mechanisms of its anti-inflammatory and anti-remodelling effects.
34536940	2	0	contain	have	381:384	arg2	properties					424:433	promising bioactive and biodegradable properties	386:433	promising bioactive and biodegradable properties	386:433	Recent studies focused on composite coatings incorporating biocompatible elements that can increase the nucleation of hydroxyapatite (HA), the mineral component of bone, and have promising bioactive and biodegradable properties.
34536940	2	0	contain	have	381:384	arg1	elements					280:287	biocompatible elements	266:287	biocompatible elements that can increase the nucleation of hydroxyapatite (HA), the mineral component of bone, and have promising bioactive and biodegradable properties	266:433	Recent studies focused on composite coatings incorporating biocompatible elements that can increase the nucleation of hydroxyapatite (HA), the mineral component of bone, and have promising bioactive and biodegradable properties.
34536940	2	1	theme	bone	371:374	arg1	component					358:366	the mineral component	346:366	the mineral component of bone	346:374	Recent studies focused on composite coatings incorporating biocompatible elements that can increase the nucleation of hydroxyapatite (HA), the mineral component of bone, and have promising bioactive and biodegradable properties.
34536940	2	1	theme	bone	371:374	arg1	hydroxyapatite					325:338	hydroxyapatite	325:338	hydroxyapatite (HA)	325:343	Recent studies focused on composite coatings incorporating biocompatible elements that can increase the nucleation of hydroxyapatite (HA), the mineral component of bone, and have promising bioactive and biodegradable properties.
34536940	7	2	theme	higher	1393:1398	arg1	concentrations					1400:1413	higher concentrations	1393:1413	higher concentrations	1393:1413	The mass of the monolayer varied depending on the polymer (collagen, CTS and collagen/CTS) and combination of polymer + PG (collagen-PG, CTS-PG and collagen/CTS-PG), while the presence of copper led to agglomerates during deposition at higher concentrations.
34536940	9	3	theme	PG	1583:1584	arg1	solution					1586:1593	the PG solution	1579:1593	the PG solution	1579:1593	Increasing the concentration of collagen in the PG solution allows for a higher deposition yield, while pure collagen solutions resulted in hydrogen gas evolution at the cathode.
34536940	7	4	theme	copper	1345:1350	arg1	presence					1333:1340	the presence	1329:1340	the presence of copper	1329:1350	The mass of the monolayer varied depending on the polymer (collagen, CTS and collagen/CTS) and combination of polymer + PG (collagen-PG, CTS-PG and collagen/CTS-PG), while the presence of copper led to agglomerates during deposition at higher concentrations.
34536940	10	5	theme	tailored	1855:1862	arg1	properties					1875:1884	tailored biological properties	1855:1884	tailored biological properties with lower risk of rejection from the host	1855:1927	The ability to deposit polymer-PG coatings that can mimic native bone tissue allows for the potential to fabricate orthopaedic implants with tailored biological properties with lower risk of rejection from the host and exhibit increased bioactivity.
34536940	1	6	theme	orthopaedic	185:195	arg1	implants					197:204	orthopaedic implants	185:204	orthopaedic implants	185:204	Coatings with bioactive properties play a key role in the success of orthopaedic implants.
34536940	5	7	theme	coating	848:854	arg1	composition					856:866	The coating composition	844:866	The coating composition	844:866	The coating composition was varied by altering the collagen/CTS concentrations in the solutions, as well as depositing PG with 0, 5 and 10 mol% CuO dopant.
34536940	9	8	theme	deposition	1615:1624	arg1	yield					1626:1630	a higher deposition yield	1606:1630	a higher deposition yield	1606:1630	Increasing the concentration of collagen in the PG solution allows for a higher deposition yield, while pure collagen solutions resulted in hydrogen gas evolution at the cathode.
34536940	10	9	theme	lower	1891:1895	arg1	risk					1897:1900	lower risk	1891:1900	lower risk of rejection from the host	1891:1927	The ability to deposit polymer-PG coatings that can mimic native bone tissue allows for the potential to fabricate orthopaedic implants with tailored biological properties with lower risk of rejection from the host and exhibit increased bioactivity.
34536940	3	10	theme	electrophoretic	585:599	arg1	EPD					613:615	EPD	613:615	EPD	613:615	Here we report a method of fabricating composite collagen, chitosan and copper-doped phosphate glass (PG) coatings for biomedical applications using electrophoretic deposition (EPD).
34536940	3	10	theme	electrophoretic	585:599	arg1	deposition					601:610	electrophoretic deposition	585:610	electrophoretic deposition (EPD)	585:616	Here we report a method of fabricating composite collagen, chitosan and copper-doped phosphate glass (PG) coatings for biomedical applications using electrophoretic deposition (EPD).
34536940	7	11	dep	polymer + PG	1267:1278	arg1	CTS-PG					1294:1299	CTS-PG	1294:1299	CTS-PG	1294:1299	The mass of the monolayer varied depending on the polymer (collagen, CTS and collagen/CTS) and combination of polymer + PG (collagen-PG, CTS-PG and collagen/CTS-PG), while the presence of copper led to agglomerates during deposition at higher concentrations.
34536940	7	11	dep	polymer + PG	1267:1278	arg1	collagen-PG					1281:1291	collagen-PG	1281:1291	collagen-PG	1281:1291	The mass of the monolayer varied depending on the polymer (collagen, CTS and collagen/CTS) and combination of polymer + PG (collagen-PG, CTS-PG and collagen/CTS-PG), while the presence of copper led to agglomerates during deposition at higher concentrations.
34536940	7	11	dep	polymer + PG	1267:1278	arg1	collagen/CTS-PG					1305:1319	collagen/CTS-PG	1305:1319	collagen/CTS-PG	1305:1319	The mass of the monolayer varied depending on the polymer (collagen, CTS and collagen/CTS) and combination of polymer + PG (collagen-PG, CTS-PG and collagen/CTS-PG), while the presence of copper led to agglomerates during deposition at higher concentrations.
34536940	6	12	with	conjunction	1117:1127	arg1	polymer					1136:1142	a polymer	1134:1142	a polymer	1134:1142	A monolayer of collagen/CTS containing PG was obtained on stainless steel cathodes, showing that deposition of PG in conjunction with a polymer is feasible.
34536940	7	13	from	concentrations	1400:1413	arg1	deposition					1379:1388	deposition	1379:1388	deposition at higher concentrations	1379:1413	The mass of the monolayer varied depending on the polymer (collagen, CTS and collagen/CTS) and combination of polymer + PG (collagen-PG, CTS-PG and collagen/CTS-PG), while the presence of copper led to agglomerates during deposition at higher concentrations.
34536940	9	14	theme	pure	1639:1642	arg1	solutions					1653:1661	pure collagen solutions	1639:1661	pure collagen solutions	1639:1661	Increasing the concentration of collagen in the PG solution allows for a higher deposition yield, while pure collagen solutions resulted in hydrogen gas evolution at the cathode.
34536940	4	15	theme	ambient	715:721	arg1	temperature					723:733	standard ambient temperature	706:733	standard ambient temperature	706:733	The use of collagen and chitosan (CTS) allows for the co-deposition of PG particles at standard ambient temperature and pressure (1 kPa, 25 °C), and the addition of collagen led to the steric stabilization of PG in solution.
34536940	5	16	theme	CuO	988:990	arg1	dopant					992:997	10 mol% CuO dopant	980:997	10 mol% CuO dopant	980:997	The coating composition was varied by altering the collagen/CTS concentrations in the solutions, as well as depositing PG with 0, 5 and 10 mol% CuO dopant.
34536940	2	17	theme	composite	233:241	arg1	coatings					243:250	composite coatings	233:250	composite coatings incorporating biocompatible elements that can increase the nucleation of hydroxyapatite (HA), the mineral component of bone, and have promising bioactive and biodegradable properties	233:433	Recent studies focused on composite coatings incorporating biocompatible elements that can increase the nucleation of hydroxyapatite (HA), the mineral component of bone, and have promising bioactive and biodegradable properties.
34536940	3	18	theme	biomedical	555:564	arg1	applications					566:577	biomedical applications	555:577	biomedical applications using electrophoretic deposition (EPD)	555:616	Here we report a method of fabricating composite collagen, chitosan and copper-doped phosphate glass (PG) coatings for biomedical applications using electrophoretic deposition (EPD).
34536940	4	19	dep	pressure	739:746	arg1	1 kPa					749:753	1 kPa	749:753	1 kPa	749:753	The use of collagen and chitosan (CTS) allows for the co-deposition of PG particles at standard ambient temperature and pressure (1 kPa, 25 °C), and the addition of collagen led to the steric stabilization of PG in solution.
34536940	4	19	dep	pressure	739:746	arg1	25 °C					756:760	25 °C	756:760	25 °C	756:760	The use of collagen and chitosan (CTS) allows for the co-deposition of PG particles at standard ambient temperature and pressure (1 kPa, 25 °C), and the addition of collagen led to the steric stabilization of PG in solution.
34536940	4	20	theme	PG	690:691	arg1	particles					693:701	PG particles	690:701	PG particles	690:701	The use of collagen and chitosan (CTS) allows for the co-deposition of PG particles at standard ambient temperature and pressure (1 kPa, 25 °C), and the addition of collagen led to the steric stabilization of PG in solution.
34536940	10	21	theme	polymer-PG	1737:1746	arg1	coatings					1748:1755	polymer-PG coatings	1737:1755	polymer-PG coatings that can mimic native bone tissue	1737:1789	The ability to deposit polymer-PG coatings that can mimic native bone tissue allows for the potential to fabricate orthopaedic implants with tailored biological properties with lower risk of rejection from the host and exhibit increased bioactivity.
34536940	9	22	theme	gas	1684:1686	arg1	evolution					1688:1696	hydrogen gas evolution	1675:1696	hydrogen gas evolution at the cathode	1675:1711	Increasing the concentration of collagen in the PG solution allows for a higher deposition yield, while pure collagen solutions resulted in hydrogen gas evolution at the cathode.
34536940	0	23	theme	orthopaedic	94:104	arg1	implants					106:113	orthopaedic implants	94:113	orthopaedic implants	94:113	Electrophoretic deposition of collagen/chitosan films with copper-doped phosphate glasses for orthopaedic implants.
34536940	6	24	contain	containing	1028:1037	arg2	PG					1039:1040	PG	1039:1040	PG	1039:1040	A monolayer of collagen/CTS containing PG was obtained on stainless steel cathodes, showing that deposition of PG in conjunction with a polymer is feasible.
34536940	6	24	contain	containing	1028:1037	arg1	collagen/CTS					1015:1026	collagen/CTS	1015:1026	collagen/CTS containing PG	1015:1040	A monolayer of collagen/CTS containing PG was obtained on stainless steel cathodes, showing that deposition of PG in conjunction with a polymer is feasible.
34536940	10	25	from	risk	1897:1900	arg1	host					1924:1927	the host	1920:1927	the host	1920:1927	The ability to deposit polymer-PG coatings that can mimic native bone tissue allows for the potential to fabricate orthopaedic implants with tailored biological properties with lower risk of rejection from the host and exhibit increased bioactivity.
34536940	1	26	theme	key	158:160	arg1	role					162:165	a key role	156:165	a key role	156:165	Coatings with bioactive properties play a key role in the success of orthopaedic implants.
34536940	2	27	theme	biodegradable	410:422	arg1	properties					424:433	promising bioactive and biodegradable properties	386:433	promising bioactive and biodegradable properties	386:433	Recent studies focused on composite coatings incorporating biocompatible elements that can increase the nucleation of hydroxyapatite (HA), the mineral component of bone, and have promising bioactive and biodegradable properties.
34536940	10	28	theme	increased	1941:1949	arg1	bioactivity					1951:1961	increased bioactivity	1941:1961	increased bioactivity	1941:1961	The ability to deposit polymer-PG coatings that can mimic native bone tissue allows for the potential to fabricate orthopaedic implants with tailored biological properties with lower risk of rejection from the host and exhibit increased bioactivity.
34536940	6	29	theme	collagen/CTS	1015:1026	arg1	monolayer					1002:1010	A monolayer	1000:1010	A monolayer of collagen/CTS containing PG	1000:1040	A monolayer of collagen/CTS containing PG was obtained on stainless steel cathodes, showing that deposition of PG in conjunction with a polymer is feasible.
34536940	8	30	theme	different	1452:1460	arg1	points					1467:1472	different time points	1452:1472	different time points	1452:1472	The deposition yield was studied at different time points and showed a profile typical of constant voltage deposition.
34536940	2	31	theme	bioactive	396:404	arg1	properties					424:433	promising bioactive and biodegradable properties	386:433	promising bioactive and biodegradable properties	386:433	Recent studies focused on composite coatings incorporating biocompatible elements that can increase the nucleation of hydroxyapatite (HA), the mineral component of bone, and have promising bioactive and biodegradable properties.
34536940	0	32	theme	Electrophoretic	0:14	arg1	deposition					16:25	Electrophoretic deposition	0:25	Electrophoretic deposition of collagen/chitosan films with copper-doped phosphate glasses for orthopaedic implants.	0:114	Electrophoretic deposition of collagen/chitosan films with copper-doped phosphate glasses for orthopaedic implants.
34536940	3	33	theme	collagen	485:492	arg1	coatings					542:549	fabricating composite collagen, chitosan and copper-doped phosphate glass (PG) coatings	463:549	fabricating composite collagen, chitosan and copper-doped phosphate glass (PG) coatings	463:549	Here we report a method of fabricating composite collagen, chitosan and copper-doped phosphate glass (PG) coatings for biomedical applications using electrophoretic deposition (EPD).
34536940	9	34	from	cathode	1705:1711	arg1	evolution					1688:1696	hydrogen gas evolution	1675:1696	hydrogen gas evolution at the cathode	1675:1711	Increasing the concentration of collagen in the PG solution allows for a higher deposition yield, while pure collagen solutions resulted in hydrogen gas evolution at the cathode.
34536940	1	35	theme	bioactive	130:138	arg1	properties					140:149	bioactive properties	130:149	bioactive properties	130:149	Coatings with bioactive properties play a key role in the success of orthopaedic implants.
34536940	10	36	theme	bone	1779:1782	arg1	tissue					1784:1789	native bone tissue	1772:1789	native bone tissue	1772:1789	The ability to deposit polymer-PG coatings that can mimic native bone tissue allows for the potential to fabricate orthopaedic implants with tailored biological properties with lower risk of rejection from the host and exhibit increased bioactivity.
34536940	6	37	from	deposition	1097:1106	arg1	conjunction					1117:1127	conjunction	1117:1127	conjunction with a polymer	1117:1142	A monolayer of collagen/CTS containing PG was obtained on stainless steel cathodes, showing that deposition of PG in conjunction with a polymer is feasible.
34536940	3	38	theme	fabricating	463:473	arg1	collagen					485:492	fabricating composite collagen	463:492	fabricating composite collagen	463:492	Here we report a method of fabricating composite collagen, chitosan and copper-doped phosphate glass (PG) coatings for biomedical applications using electrophoretic deposition (EPD).
34536940	0	39	theme	films	48:52	arg1	deposition					16:25	Electrophoretic deposition	0:25	Electrophoretic deposition of collagen/chitosan films with copper-doped phosphate glasses for orthopaedic implants.	0:114	Electrophoretic deposition of collagen/chitosan films with copper-doped phosphate glasses for orthopaedic implants.
34536940	4	40	theme	steric	804:809	arg1	stabilization					811:823	the steric stabilization	800:823	the steric stabilization of PG in solution	800:841	The use of collagen and chitosan (CTS) allows for the co-deposition of PG particles at standard ambient temperature and pressure (1 kPa, 25 °C), and the addition of collagen led to the steric stabilization of PG in solution.
34536940	3	41	theme	copper-doped	508:519	arg1	PG					538:539	PG	538:539	PG	538:539	Here we report a method of fabricating composite collagen, chitosan and copper-doped phosphate glass (PG) coatings for biomedical applications using electrophoretic deposition (EPD).
34536940	3	41	theme	copper-doped	508:519	arg1	glass					531:535	copper-doped phosphate glass	508:535	copper-doped phosphate glass (PG)	508:540	Here we report a method of fabricating composite collagen, chitosan and copper-doped phosphate glass (PG) coatings for biomedical applications using electrophoretic deposition (EPD).
34536940	0	42	theme	copper-doped	59:70	arg1	glasses					82:88	copper-doped phosphate glasses	59:88	copper-doped phosphate glasses for orthopaedic implants	59:113	Electrophoretic deposition of collagen/chitosan films with copper-doped phosphate glasses for orthopaedic implants.
34536940	5	43	theme	%	986:986	arg1	dopant					992:997	10 mol% CuO dopant	980:997	10 mol% CuO dopant	980:997	The coating composition was varied by altering the collagen/CTS concentrations in the solutions, as well as depositing PG with 0, 5 and 10 mol% CuO dopant.
34536940	2	44	theme	mineral	350:356	arg1	component					358:366	the mineral component	346:366	the mineral component of bone	346:374	Recent studies focused on composite coatings incorporating biocompatible elements that can increase the nucleation of hydroxyapatite (HA), the mineral component of bone, and have promising bioactive and biodegradable properties.
34536940	2	44	theme	mineral	350:356	arg1	hydroxyapatite					325:338	hydroxyapatite	325:338	hydroxyapatite (HA)	325:343	Recent studies focused on composite coatings incorporating biocompatible elements that can increase the nucleation of hydroxyapatite (HA), the mineral component of bone, and have promising bioactive and biodegradable properties.
34536940	6	45	theme	stainless	1058:1066	arg1	cathodes					1074:1081	stainless steel cathodes	1058:1081	stainless steel cathodes	1058:1081	A monolayer of collagen/CTS containing PG was obtained on stainless steel cathodes, showing that deposition of PG in conjunction with a polymer is feasible.
34536940	8	46	theme	voltage	1515:1521	arg1	deposition					1523:1532	constant voltage deposition	1506:1532	constant voltage deposition	1506:1532	The deposition yield was studied at different time points and showed a profile typical of constant voltage deposition.
34536940	2	47	theme	Recent	207:212	arg1	studies					214:220	Recent studies	207:220	Recent studies	207:220	Recent studies focused on composite coatings incorporating biocompatible elements that can increase the nucleation of hydroxyapatite (HA), the mineral component of bone, and have promising bioactive and biodegradable properties.
34536940	4	48	theme	collagen	784:791	arg1	addition					772:779	the addition	768:779	the addition of collagen	768:791	The use of collagen and chitosan (CTS) allows for the co-deposition of PG particles at standard ambient temperature and pressure (1 kPa, 25 °C), and the addition of collagen led to the steric stabilization of PG in solution.
34536940	9	49	theme	collagen	1567:1574	arg1	concentration					1550:1562	the concentration	1546:1562	the concentration of collagen in the PG solution	1546:1593	Increasing the concentration of collagen in the PG solution allows for a higher deposition yield, while pure collagen solutions resulted in hydrogen gas evolution at the cathode.
34536940	4	50	theme	collagen	630:637	arg1	use					623:625	The use	619:625	The use of collagen and chitosan (CTS)	619:656	The use of collagen and chitosan (CTS) allows for the co-deposition of PG particles at standard ambient temperature and pressure (1 kPa, 25 °C), and the addition of collagen led to the steric stabilization of PG in solution.
34536940	10	51	theme	orthopaedic	1829:1839	arg1	implants					1841:1848	orthopaedic implants	1829:1848	orthopaedic implants with tailored biological properties with lower risk of rejection from the host	1829:1927	The ability to deposit polymer-PG coatings that can mimic native bone tissue allows for the potential to fabricate orthopaedic implants with tailored biological properties with lower risk of rejection from the host and exhibit increased bioactivity.
34536940	3	52	theme	glass	531:535	arg1	coatings					542:549	fabricating composite collagen, chitosan and copper-doped phosphate glass (PG) coatings	463:549	fabricating composite collagen, chitosan and copper-doped phosphate glass (PG) coatings	463:549	Here we report a method of fabricating composite collagen, chitosan and copper-doped phosphate glass (PG) coatings for biomedical applications using electrophoretic deposition (EPD).
34536940	7	53	theme	monolayer	1173:1181	arg1	mass					1161:1164	The mass	1157:1164	The mass of the monolayer	1157:1181	The mass of the monolayer varied depending on the polymer (collagen, CTS and collagen/CTS) and combination of polymer + PG (collagen-PG, CTS-PG and collagen/CTS-PG), while the presence of copper led to agglomerates during deposition at higher concentrations.
34536940	2	54	theme	hydroxyapatite	325:338	arg1	nucleation					311:320	the nucleation	307:320	the nucleation of hydroxyapatite (HA), the mineral component of bone	307:374	Recent studies focused on composite coatings incorporating biocompatible elements that can increase the nucleation of hydroxyapatite (HA), the mineral component of bone, and have promising bioactive and biodegradable properties.
34536940	10	55	theme	biological	1864:1873	arg1	properties					1875:1884	tailored biological properties	1855:1884	tailored biological properties with lower risk of rejection from the host	1855:1927	The ability to deposit polymer-PG coatings that can mimic native bone tissue allows for the potential to fabricate orthopaedic implants with tailored biological properties with lower risk of rejection from the host and exhibit increased bioactivity.
34536940	3	56	theme	coatings	542:549	arg1	method					453:458	a method	451:458	a method of fabricating composite collagen, chitosan and copper-doped phosphate glass (PG) coatings for biomedical applications using electrophoretic deposition (EPD)	451:616	Here we report a method of fabricating composite collagen, chitosan and copper-doped phosphate glass (PG) coatings for biomedical applications using electrophoretic deposition (EPD).
34536940	6	57	theme	PG	1111:1112	arg1	deposition					1097:1106	deposition	1097:1106	deposition of PG in conjunction with a polymer	1097:1142	A monolayer of collagen/CTS containing PG was obtained on stainless steel cathodes, showing that deposition of PG in conjunction with a polymer is feasible.
34536940	1	58	theme	implants	197:204	arg1	success					174:180	the success	170:180	the success of orthopaedic implants	170:204	Coatings with bioactive properties play a key role in the success of orthopaedic implants.
34536940	4	59	theme	PG	828:829	arg1	stabilization					811:823	the steric stabilization	800:823	the steric stabilization of PG in solution	800:841	The use of collagen and chitosan (CTS) allows for the co-deposition of PG particles at standard ambient temperature and pressure (1 kPa, 25 °C), and the addition of collagen led to the steric stabilization of PG in solution.
34536940	1	60	with	Coatings	116:123	arg1	properties					140:149	bioactive properties	130:149	bioactive properties	130:149	Coatings with bioactive properties play a key role in the success of orthopaedic implants.
34536940	4	61	theme	chitosan	643:650	arg1	use					623:625	The use	619:625	The use of collagen and chitosan (CTS)	619:656	The use of collagen and chitosan (CTS) allows for the co-deposition of PG particles at standard ambient temperature and pressure (1 kPa, 25 °C), and the addition of collagen led to the steric stabilization of PG in solution.
34536940	9	62	theme	higher	1608:1613	arg1	yield					1626:1630	a higher deposition yield	1606:1630	a higher deposition yield	1606:1630	Increasing the concentration of collagen in the PG solution allows for a higher deposition yield, while pure collagen solutions resulted in hydrogen gas evolution at the cathode.
34536940	2	63	theme	biocompatible	266:278	arg1	elements					280:287	biocompatible elements	266:287	biocompatible elements that can increase the nucleation of hydroxyapatite (HA), the mineral component of bone, and have promising bioactive and biodegradable properties	266:433	Recent studies focused on composite coatings incorporating biocompatible elements that can increase the nucleation of hydroxyapatite (HA), the mineral component of bone, and have promising bioactive and biodegradable properties.
34536940	9	64	theme	collagen	1644:1651	arg1	solutions					1653:1661	pure collagen solutions	1639:1661	pure collagen solutions	1639:1661	Increasing the concentration of collagen in the PG solution allows for a higher deposition yield, while pure collagen solutions resulted in hydrogen gas evolution at the cathode.
34536940	4	65	theme	standard	706:713	arg1	temperature					723:733	standard ambient temperature	706:733	standard ambient temperature	706:733	The use of collagen and chitosan (CTS) allows for the co-deposition of PG particles at standard ambient temperature and pressure (1 kPa, 25 °C), and the addition of collagen led to the steric stabilization of PG in solution.
34536940	10	66	theme	rejection	1905:1913	arg1	risk					1897:1900	lower risk	1891:1900	lower risk of rejection from the host	1891:1927	The ability to deposit polymer-PG coatings that can mimic native bone tissue allows for the potential to fabricate orthopaedic implants with tailored biological properties with lower risk of rejection from the host and exhibit increased bioactivity.
34536940	5	67	theme	collagen/CTS	895:906	arg1	concentrations					908:921	the collagen/CTS concentrations	891:921	the collagen/CTS concentrations in the solutions	891:938	The coating composition was varied by altering the collagen/CTS concentrations in the solutions, as well as depositing PG with 0, 5 and 10 mol% CuO dopant.
34536940	3	68	theme	chitosan	495:502	arg1	coatings					542:549	fabricating composite collagen, chitosan and copper-doped phosphate glass (PG) coatings	463:549	fabricating composite collagen, chitosan and copper-doped phosphate glass (PG) coatings	463:549	Here we report a method of fabricating composite collagen, chitosan and copper-doped phosphate glass (PG) coatings for biomedical applications using electrophoretic deposition (EPD).
34536940	9	69	theme	hydrogen	1675:1682	arg1	evolution					1688:1696	hydrogen gas evolution	1675:1696	hydrogen gas evolution at the cathode	1675:1711	Increasing the concentration of collagen in the PG solution allows for a higher deposition yield, while pure collagen solutions resulted in hydrogen gas evolution at the cathode.
34536940	8	70	theme	deposition	1420:1429	arg1	yield					1431:1435	The deposition yield	1416:1435	The deposition yield	1416:1435	The deposition yield was studied at different time points and showed a profile typical of constant voltage deposition.
34536940	7	71	theme	polymer + PG	1267:1278	arg1	polymer					1207:1213	the polymer	1203:1213	the polymer (collagen, CTS and collagen/CTS)	1203:1246	The mass of the monolayer varied depending on the polymer (collagen, CTS and collagen/CTS) and combination of polymer + PG (collagen-PG, CTS-PG and collagen/CTS-PG), while the presence of copper led to agglomerates during deposition at higher concentrations.
34536940	7	71	theme	polymer + PG	1267:1278	arg1	combination					1252:1262	combination	1252:1262	combination of polymer + PG (collagen-PG, CTS-PG and collagen/CTS-PG)	1252:1320	The mass of the monolayer varied depending on the polymer (collagen, CTS and collagen/CTS) and combination of polymer + PG (collagen-PG, CTS-PG and collagen/CTS-PG), while the presence of copper led to agglomerates during deposition at higher concentrations.
34536940	4	72	theme	particles	693:701	arg1	co-deposition					673:685	the co-deposition	669:685	the co-deposition of PG particles	669:701	The use of collagen and chitosan (CTS) allows for the co-deposition of PG particles at standard ambient temperature and pressure (1 kPa, 25 °C), and the addition of collagen led to the steric stabilization of PG in solution.
34536940	7	73	dep	polymer	1207:1213	arg1	collagen					1216:1223	collagen	1216:1223	collagen	1216:1223	The mass of the monolayer varied depending on the polymer (collagen, CTS and collagen/CTS) and combination of polymer + PG (collagen-PG, CTS-PG and collagen/CTS-PG), while the presence of copper led to agglomerates during deposition at higher concentrations.
34536940	8	74	theme	time	1462:1465	arg1	points					1467:1472	different time points	1452:1472	different time points	1452:1472	The deposition yield was studied at different time points and showed a profile typical of constant voltage deposition.
34536940	4	75	from	stabilization	811:823	arg1	solution					834:841	solution	834:841	solution	834:841	The use of collagen and chitosan (CTS) allows for the co-deposition of PG particles at standard ambient temperature and pressure (1 kPa, 25 °C), and the addition of collagen led to the steric stabilization of PG in solution.
34536940	10	76	from	host	1924:1927	arg1	risk					1897:1900	lower risk	1891:1900	lower risk of rejection from the host	1891:1927	The ability to deposit polymer-PG coatings that can mimic native bone tissue allows for the potential to fabricate orthopaedic implants with tailored biological properties with lower risk of rejection from the host and exhibit increased bioactivity.
34536940	10	76	from	host	1924:1927	arg1	rejection					1905:1913	rejection	1905:1913	rejection from the host	1905:1927	The ability to deposit polymer-PG coatings that can mimic native bone tissue allows for the potential to fabricate orthopaedic implants with tailored biological properties with lower risk of rejection from the host and exhibit increased bioactivity.
34536940	5	77	from	concentrations	908:921	arg1	solutions					930:938	the solutions	926:938	the solutions	926:938	The coating composition was varied by altering the collagen/CTS concentrations in the solutions, as well as depositing PG with 0, 5 and 10 mol% CuO dopant.
34536940	9	78	from	concentration	1550:1562	arg1	solution					1586:1593	the PG solution	1579:1593	the PG solution	1579:1593	Increasing the concentration of collagen in the PG solution allows for a higher deposition yield, while pure collagen solutions resulted in hydrogen gas evolution at the cathode.
34536940	10	79	with	implants	1841:1848	arg1	properties					1875:1884	tailored biological properties	1855:1884	tailored biological properties with lower risk of rejection from the host	1855:1927	The ability to deposit polymer-PG coatings that can mimic native bone tissue allows for the potential to fabricate orthopaedic implants with tailored biological properties with lower risk of rejection from the host and exhibit increased bioactivity.
34536940	2	80	theme	promising	386:394	arg1	properties					424:433	promising bioactive and biodegradable properties	386:433	promising bioactive and biodegradable properties	386:433	Recent studies focused on composite coatings incorporating biocompatible elements that can increase the nucleation of hydroxyapatite (HA), the mineral component of bone, and have promising bioactive and biodegradable properties.
34536940	0	81	theme	collagen/chitosan	30:46	arg1	films					48:52	collagen/chitosan films	30:52	collagen/chitosan films	30:52	Electrophoretic deposition of collagen/chitosan films with copper-doped phosphate glasses for orthopaedic implants.
34536940	10	82	with	properties	1875:1884	arg1	risk					1897:1900	lower risk	1891:1900	lower risk of rejection from the host	1891:1927	The ability to deposit polymer-PG coatings that can mimic native bone tissue allows for the potential to fabricate orthopaedic implants with tailored biological properties with lower risk of rejection from the host and exhibit increased bioactivity.
34536940	8	83	theme	typical	1495:1501	arg1	profile					1487:1493	a profile	1485:1493	a profile typical of constant voltage deposition	1485:1532	The deposition yield was studied at different time points and showed a profile typical of constant voltage deposition.
34536940	10	84	theme	native	1772:1777	arg1	tissue					1784:1789	native bone tissue	1772:1789	native bone tissue	1772:1789	The ability to deposit polymer-PG coatings that can mimic native bone tissue allows for the potential to fabricate orthopaedic implants with tailored biological properties with lower risk of rejection from the host and exhibit increased bioactivity.
34536940	3	85	theme	composite	475:483	arg1	collagen					485:492	fabricating composite collagen	463:492	fabricating composite collagen	463:492	Here we report a method of fabricating composite collagen, chitosan and copper-doped phosphate glass (PG) coatings for biomedical applications using electrophoretic deposition (EPD).
34536940	0	86	theme	phosphate	72:80	arg1	glasses					82:88	copper-doped phosphate glasses	59:88	copper-doped phosphate glasses for orthopaedic implants	59:113	Electrophoretic deposition of collagen/chitosan films with copper-doped phosphate glasses for orthopaedic implants.
34536940	5	87	theme	10 mol	980:985	arg1	dopant					992:997	10 mol% CuO dopant	980:997	10 mol% CuO dopant	980:997	The coating composition was varied by altering the collagen/CTS concentrations in the solutions, as well as depositing PG with 0, 5 and 10 mol% CuO dopant.
34536940	8	88	theme	constant	1506:1513	arg1	deposition					1523:1532	constant voltage deposition	1506:1532	constant voltage deposition	1506:1532	The deposition yield was studied at different time points and showed a profile typical of constant voltage deposition.
34536940	0	89	with	deposition	16:25	arg1	glasses					82:88	copper-doped phosphate glasses	59:88	copper-doped phosphate glasses for orthopaedic implants	59:113	Electrophoretic deposition of collagen/chitosan films with copper-doped phosphate glasses for orthopaedic implants.
34536940	3	90	theme	phosphate	521:529	arg1	PG					538:539	PG	538:539	PG	538:539	Here we report a method of fabricating composite collagen, chitosan and copper-doped phosphate glass (PG) coatings for biomedical applications using electrophoretic deposition (EPD).
34536940	3	90	theme	phosphate	521:529	arg1	glass					531:535	copper-doped phosphate glass	508:535	copper-doped phosphate glass (PG)	508:540	Here we report a method of fabricating composite collagen, chitosan and copper-doped phosphate glass (PG) coatings for biomedical applications using electrophoretic deposition (EPD).
34536940	7	91	dep	collagen	1216:1223	arg1	CTS					1226:1228	CTS	1226:1228	CTS	1226:1228	The mass of the monolayer varied depending on the polymer (collagen, CTS and collagen/CTS) and combination of polymer + PG (collagen-PG, CTS-PG and collagen/CTS-PG), while the presence of copper led to agglomerates during deposition at higher concentrations.
34536940	7	91	dep	collagen	1216:1223	arg1	collagen/CTS					1234:1245	collagen/CTS	1234:1245	collagen/CTS	1234:1245	The mass of the monolayer varied depending on the polymer (collagen, CTS and collagen/CTS) and combination of polymer + PG (collagen-PG, CTS-PG and collagen/CTS-PG), while the presence of copper led to agglomerates during deposition at higher concentrations.
34536940	6	92	theme	steel	1068:1072	arg1	cathodes					1074:1081	stainless steel cathodes	1058:1081	stainless steel cathodes	1058:1081	A monolayer of collagen/CTS containing PG was obtained on stainless steel cathodes, showing that deposition of PG in conjunction with a polymer is feasible.
34536940	8	93	theme	deposition	1523:1532	arg1	typical					1495:1501	typical	1495:1501	typical	1495:1501	The deposition yield was studied at different time points and showed a profile typical of constant voltage deposition.
34008607	8	0	with	treatment	1181:1189	arg1	PSPPs					1196:1200	PSPPs	1196:1200	PSPPs	1196:1200	In addition, treatment with PSPPs decreased the levels of IL-1β, IL-6 and TNF-α but increased the level of IL-10 in the intestines of mice (p < 0.01).
34008607	5	1	theme	0.4	863:865	arg1	g					867:867	g	867:867	g	867:867	The diarrhea model was established via intragastric administration of lincomycin and different concentrations of PSPPs (0.1 g kg-1, 0.2 g kg-1, and 0.4 g kg-1) to Balb/C mice.
34008607	1	2	theme	flora	269:273	arg1	disorders					241:249	disorders	241:249	disorders of the intestinal flora	241:273	Antibiotic treatment causes antibiotic-associated diarrhea (AAD), which is usually accompanied by disorders of the intestinal flora, aggravating the patient's condition.
34008607	0	3	theme	intestinal	76:85	arg1	microbiota					87:96	intestinal microbiota	76:96	intestinal microbiota of mice with antibiotic-associated diarrhea	76:140	Regulatory effect of non-starch polysaccharides from purple sweet potato on intestinal microbiota of mice with antibiotic-associated diarrhea.
34008607	0	4	from	effect	11:16	arg1	potato					66:71	purple sweet potato	53:71	purple sweet potato	53:71	Regulatory effect of non-starch polysaccharides from purple sweet potato on intestinal microbiota of mice with antibiotic-associated diarrhea.
34008607	0	4	from	effect	11:16	arg1	microbiota					87:96	intestinal microbiota	76:96	intestinal microbiota of mice with antibiotic-associated diarrhea	76:140	Regulatory effect of non-starch polysaccharides from purple sweet potato on intestinal microbiota of mice with antibiotic-associated diarrhea.
34008607	7	5	dep	In	1025:1026	arg1	vivo					1028:1031	vivo	1028:1031	vivo	1028:1031	In vivo experiments showed that PSPP could relieve diarrhea and improve the structural damage in the ileum caused by lincomycin hydrochloride.
34008607	4	6	theme	intestinal	680:689	arg1	flora					691:695	the intestinal flora	676:695	the intestinal flora of mice with AAD	676:712	This study aimed to extract a non-starch polysaccharide from purple sweet potato and analyze its structure and ability to regulate the intestinal flora of mice with AAD.
34008607	0	7	theme	mice	101:104	arg1	microbiota					87:96	intestinal microbiota	76:96	intestinal microbiota of mice with antibiotic-associated diarrhea	76:140	Regulatory effect of non-starch polysaccharides from purple sweet potato on intestinal microbiota of mice with antibiotic-associated diarrhea.
34008607	10	8	theme	fatty	1517:1521	arg1	acids					1523:1527	short-chain fatty acids	1505:1527	short-chain fatty acids (p < 0.01)	1505:1538	In addition, PSPP treatment increased the content of short-chain fatty acids (p < 0.01).
34008607	10	8	theme	fatty	1517:1521	arg1	<					1532:1532	p < 0.01	1530:1537	p < 0.01	1530:1537	In addition, PSPP treatment increased the content of short-chain fatty acids (p < 0.01).
34008607	9	9	theme	sequencing	1343:1352	arg1	results					1323:1329	The results	1319:1329	The results of 16S rRNA sequencing	1319:1352	The results of 16S rRNA sequencing showed that PSPPs changed the composition and diversity of the intestinal flora of mice with AAD.
34008607	4	10	theme	non-starch	575:584	arg1	polysaccharide					586:599	a non-starch polysaccharide	573:599	a non-starch polysaccharide from purple sweet potato	573:624	This study aimed to extract a non-starch polysaccharide from purple sweet potato and analyze its structure and ability to regulate the intestinal flora of mice with AAD.
34008607	7	11	theme	structural	1101:1110	arg1	damage					1112:1117	the structural damage	1097:1117	the structural damage	1097:1117	In vivo experiments showed that PSPP could relieve diarrhea and improve the structural damage in the ileum caused by lincomycin hydrochloride.
34008607	9	12	theme	flora	1428:1432	arg1	diversity					1400:1408	diversity	1400:1408	diversity	1400:1408	The results of 16S rRNA sequencing showed that PSPPs changed the composition and diversity of the intestinal flora of mice with AAD.
34008607	9	12	theme	flora	1428:1432	arg1	composition					1384:1394	composition	1384:1394	composition	1384:1394	The results of 16S rRNA sequencing showed that PSPPs changed the composition and diversity of the intestinal flora of mice with AAD.
34008607	5	13	theme	Balb/C	878:883	arg1	mice					885:888	Balb/C mice	878:888	Balb/C mice	878:888	The diarrhea model was established via intragastric administration of lincomycin and different concentrations of PSPPs (0.1 g kg-1, 0.2 g kg-1, and 0.4 g kg-1) to Balb/C mice.
34008607	0	14	theme	antibiotic-associated	111:131	arg1	diarrhea					133:140	antibiotic-associated diarrhea	111:140	antibiotic-associated diarrhea	111:140	Regulatory effect of non-starch polysaccharides from purple sweet potato on intestinal microbiota of mice with antibiotic-associated diarrhea.
34008607	3	15	theme	potato	486:491	arg1	PSPPs					510:514	PSPPs	510:514	PSPPs	510:514	However, reports on whether purple sweet potato polysaccharides (PSPPs) can improve AAD are scarce.
34008607	3	15	theme	potato	486:491	arg1	polysaccharides					493:507	purple sweet potato polysaccharides	473:507	purple sweet potato polysaccharides (PSPPs)	473:515	However, reports on whether purple sweet potato polysaccharides (PSPPs) can improve AAD are scarce.
34008607	7	16	theme	In	1025:1026	arg1	experiments					1033:1043	In vivo experiments	1025:1043	In vivo experiments	1025:1043	In vivo experiments showed that PSPP could relieve diarrhea and improve the structural damage in the ileum caused by lincomycin hydrochloride.
34008607	11	17	theme	diarrhea	1668:1675	arg1	symptoms					1656:1663	the symptoms	1652:1663	the symptoms of diarrhea	1652:1675	These results revealed that PSPPs regulated the intestinal flora, balanced fatty acid metabolism, and relieved the symptoms of diarrhea to a certain extent in mice.
34008607	4	18	with	mice	700:703	arg1	AAD					710:712	AAD	710:712	AAD	710:712	This study aimed to extract a non-starch polysaccharide from purple sweet potato and analyze its structure and ability to regulate the intestinal flora of mice with AAD.
34008607	5	19	theme	concentrations	810:823	arg1	administration					767:780	intragastric administration	754:780	intragastric administration of lincomycin and different concentrations of PSPPs (0.1 g kg-1, 0.2 g kg-1, and 0.4 g kg-1) to Balb/C mice	754:888	The diarrhea model was established via intragastric administration of lincomycin and different concentrations of PSPPs (0.1 g kg-1, 0.2 g kg-1, and 0.4 g kg-1) to Balb/C mice.
34008607	5	20	theme	0.2	847:849	arg1	g					851:851	g	851:851	g	851:851	The diarrhea model was established via intragastric administration of lincomycin and different concentrations of PSPPs (0.1 g kg-1, 0.2 g kg-1, and 0.4 g kg-1) to Balb/C mice.
34008607	5	21	theme	different	800:808	arg1	concentrations					810:823	different concentrations	800:823	different concentrations of PSPPs (0.1 g kg-1, 0.2 g kg-1, and 0.4 g kg-1)	800:873	The diarrhea model was established via intragastric administration of lincomycin and different concentrations of PSPPs (0.1 g kg-1, 0.2 g kg-1, and 0.4 g kg-1) to Balb/C mice.
34008607	5	22	theme	intragastric	754:765	arg1	administration					767:780	intragastric administration	754:780	intragastric administration of lincomycin and different concentrations of PSPPs (0.1 g kg-1, 0.2 g kg-1, and 0.4 g kg-1) to Balb/C mice	754:888	The diarrhea model was established via intragastric administration of lincomycin and different concentrations of PSPPs (0.1 g kg-1, 0.2 g kg-1, and 0.4 g kg-1) to Balb/C mice.
34008607	9	23	theme	16S	1334:1336	arg1	sequencing					1343:1352	16S rRNA sequencing	1334:1352	16S rRNA sequencing	1334:1352	The results of 16S rRNA sequencing showed that PSPPs changed the composition and diversity of the intestinal flora of mice with AAD.
34008607	8	24	theme	IL-1β	1226:1230	arg1	levels					1216:1221	the levels	1212:1221	the levels of IL-1β, IL-6 and TNF-α	1212:1246	In addition, treatment with PSPPs decreased the levels of IL-1β, IL-6 and TNF-α but increased the level of IL-10 in the intestines of mice (p < 0.01).
34008607	5	25	theme	g	851:851	arg1	kg-1					853:856	0.2 g kg-1	847:856	0.2 g kg-1	847:856	The diarrhea model was established via intragastric administration of lincomycin and different concentrations of PSPPs (0.1 g kg-1, 0.2 g kg-1, and 0.4 g kg-1) to Balb/C mice.
34008607	0	26	theme	Regulatory	0:9	arg1	effect					11:16	Regulatory effect	0:16	Regulatory effect of non-starch polysaccharides from purple sweet potato on intestinal microbiota of mice with antibiotic-associated diarrhea	0:140	Regulatory effect of non-starch polysaccharides from purple sweet potato on intestinal microbiota of mice with antibiotic-associated diarrhea.
34008607	0	27	with	mice	101:104	arg1	diarrhea					133:140	antibiotic-associated diarrhea	111:140	antibiotic-associated diarrhea	111:140	Regulatory effect of non-starch polysaccharides from purple sweet potato on intestinal microbiota of mice with antibiotic-associated diarrhea.
34008607	2	28	theme	polysaccharides	379:393	arg1	ability					362:368	the ability	358:368	the ability of plant polysaccharides to improve the body's flora and enhance immunity	358:442	Recently, more attention has been devoted to the ability of plant polysaccharides to improve the body's flora and enhance immunity.
34008607	0	29	theme	non-starch	21:30	arg1	polysaccharides					32:46	non-starch polysaccharides	21:46	non-starch polysaccharides from purple sweet potato	21:71	Regulatory effect of non-starch polysaccharides from purple sweet potato on intestinal microbiota of mice with antibiotic-associated diarrhea.
34008607	9	30	theme	mice	1437:1440	arg1	flora					1428:1432	the intestinal flora	1413:1432	the intestinal flora of mice with AAD	1413:1449	The results of 16S rRNA sequencing showed that PSPPs changed the composition and diversity of the intestinal flora of mice with AAD.
34008607	9	31	theme	intestinal	1417:1426	arg1	flora					1428:1432	the intestinal flora	1413:1432	the intestinal flora of mice with AAD	1413:1449	The results of 16S rRNA sequencing showed that PSPPs changed the composition and diversity of the intestinal flora of mice with AAD.
34008607	8	32	theme	IL-6	1233:1236	arg1	levels					1216:1221	the levels	1212:1221	the levels of IL-1β, IL-6 and TNF-α	1212:1246	In addition, treatment with PSPPs decreased the levels of IL-1β, IL-6 and TNF-α but increased the level of IL-10 in the intestines of mice (p < 0.01).
34008607	6	33	from	bonds	996:1000	arg1	α-configuration					1008:1022	an α-configuration	1005:1022	an α-configuration	1005:1022	The results showed that PSPP was a pyran polysaccharide with 1 → 2, 1 → 2, 6, 1 → 4, 1 → 4, 6 glycosidic bonds in an α-configuration.
34008607	5	34	theme	PSPPs	828:832	arg1	lincomycin					785:794	lincomycin	785:794	lincomycin	785:794	The diarrhea model was established via intragastric administration of lincomycin and different concentrations of PSPPs (0.1 g kg-1, 0.2 g kg-1, and 0.4 g kg-1) to Balb/C mice.
34008607	5	34	theme	PSPPs	828:832	arg1	concentrations					810:823	different concentrations	800:823	different concentrations of PSPPs (0.1 g kg-1, 0.2 g kg-1, and 0.4 g kg-1)	800:873	The diarrhea model was established via intragastric administration of lincomycin and different concentrations of PSPPs (0.1 g kg-1, 0.2 g kg-1, and 0.4 g kg-1) to Balb/C mice.
34008607	10	35	theme	p	1530:1530	arg1	acids					1523:1527	short-chain fatty acids	1505:1527	short-chain fatty acids (p < 0.01)	1505:1538	In addition, PSPP treatment increased the content of short-chain fatty acids (p < 0.01).
34008607	10	35	theme	p	1530:1530	arg1	<					1532:1532	p < 0.01	1530:1537	p < 0.01	1530:1537	In addition, PSPP treatment increased the content of short-chain fatty acids (p < 0.01).
34008607	5	36	theme	g	867:867	arg1	kg-1					869:872	0.4 g kg-1	863:872	0.4 g kg-1	863:872	The diarrhea model was established via intragastric administration of lincomycin and different concentrations of PSPPs (0.1 g kg-1, 0.2 g kg-1, and 0.4 g kg-1) to Balb/C mice.
34008607	9	37	theme	rRNA	1338:1341	arg1	sequencing					1343:1352	16S rRNA sequencing	1334:1352	16S rRNA sequencing	1334:1352	The results of 16S rRNA sequencing showed that PSPPs changed the composition and diversity of the intestinal flora of mice with AAD.
34008607	8	38	dep	increased	1252:1260	arg1	<					1310:1310	p < 0.01	1308:1315	p < 0.01	1308:1315	In addition, treatment with PSPPs decreased the levels of IL-1β, IL-6 and TNF-α but increased the level of IL-10 in the intestines of mice (p < 0.01).
34008607	10	39	theme	PSPP	1465:1468	arg1	treatment					1470:1478	PSPP treatment	1465:1478	PSPP treatment	1465:1478	In addition, PSPP treatment increased the content of short-chain fatty acids (p < 0.01).
34008607	8	40	theme	TNF-α	1242:1246	arg1	levels					1216:1221	the levels	1212:1221	the levels of IL-1β, IL-6 and TNF-α	1212:1246	In addition, treatment with PSPPs decreased the levels of IL-1β, IL-6 and TNF-α but increased the level of IL-10 in the intestines of mice (p < 0.01).
34008607	4	41	from	potato	619:624	arg1	polysaccharide					586:599	a non-starch polysaccharide	573:599	a non-starch polysaccharide from purple sweet potato	573:624	This study aimed to extract a non-starch polysaccharide from purple sweet potato and analyze its structure and ability to regulate the intestinal flora of mice with AAD.
34008607	7	42	theme	lincomycin	1142:1151	arg1	hydrochloride					1153:1165	lincomycin hydrochloride	1142:1165	lincomycin hydrochloride	1142:1165	In vivo experiments showed that PSPP could relieve diarrhea and improve the structural damage in the ileum caused by lincomycin hydrochloride.
34008607	0	43	theme	polysaccharides	32:46	arg1	effect					11:16	Regulatory effect	0:16	Regulatory effect of non-starch polysaccharides from purple sweet potato on intestinal microbiota of mice with antibiotic-associated diarrhea	0:140	Regulatory effect of non-starch polysaccharides from purple sweet potato on intestinal microbiota of mice with antibiotic-associated diarrhea.
34008607	11	44	theme	balanced	1607:1614	arg1	metabolism					1627:1636	balanced fatty acid metabolism	1607:1636	balanced fatty acid metabolism	1607:1636	These results revealed that PSPPs regulated the intestinal flora, balanced fatty acid metabolism, and relieved the symptoms of diarrhea to a certain extent in mice.
34008607	11	44	theme	balanced	1607:1614	arg1	flora					1600:1604	the intestinal flora	1585:1604	the intestinal flora	1585:1604	These results revealed that PSPPs regulated the intestinal flora, balanced fatty acid metabolism, and relieved the symptoms of diarrhea to a certain extent in mice.
34008607	11	45	from	extent	1690:1695	arg1	mice					1700:1703	mice	1700:1703	mice	1700:1703	These results revealed that PSPPs regulated the intestinal flora, balanced fatty acid metabolism, and relieved the symptoms of diarrhea to a certain extent in mice.
34008607	0	46	theme	sweet	60:64	arg1	potato					66:71	purple sweet potato	53:71	purple sweet potato	53:71	Regulatory effect of non-starch polysaccharides from purple sweet potato on intestinal microbiota of mice with antibiotic-associated diarrhea.
34008607	11	47	theme	fatty	1616:1620	arg1	metabolism					1627:1636	balanced fatty acid metabolism	1607:1636	balanced fatty acid metabolism	1607:1636	These results revealed that PSPPs regulated the intestinal flora, balanced fatty acid metabolism, and relieved the symptoms of diarrhea to a certain extent in mice.
34008607	11	47	theme	fatty	1616:1620	arg1	flora					1600:1604	the intestinal flora	1585:1604	the intestinal flora	1585:1604	These results revealed that PSPPs regulated the intestinal flora, balanced fatty acid metabolism, and relieved the symptoms of diarrhea to a certain extent in mice.
34008607	0	48	from	potato	66:71	arg1	effect					11:16	Regulatory effect	0:16	Regulatory effect of non-starch polysaccharides from purple sweet potato on intestinal microbiota of mice with antibiotic-associated diarrhea	0:140	Regulatory effect of non-starch polysaccharides from purple sweet potato on intestinal microbiota of mice with antibiotic-associated diarrhea.
34008607	0	48	from	potato	66:71	arg1	polysaccharides					32:46	non-starch polysaccharides	21:46	non-starch polysaccharides from purple sweet potato	21:71	Regulatory effect of non-starch polysaccharides from purple sweet potato on intestinal microbiota of mice with antibiotic-associated diarrhea.
34008607	10	49	theme	short-chain	1505:1515	arg1	acids					1523:1527	short-chain fatty acids	1505:1527	short-chain fatty acids (p < 0.01)	1505:1538	In addition, PSPP treatment increased the content of short-chain fatty acids (p < 0.01).
34008607	10	49	theme	short-chain	1505:1515	arg1	<					1532:1532	p < 0.01	1530:1537	p < 0.01	1530:1537	In addition, PSPP treatment increased the content of short-chain fatty acids (p < 0.01).
34008607	4	50	theme	mice	700:703	arg1	flora					691:695	the intestinal flora	676:695	the intestinal flora of mice with AAD	676:712	This study aimed to extract a non-starch polysaccharide from purple sweet potato and analyze its structure and ability to regulate the intestinal flora of mice with AAD.
34008607	0	51	theme	purple	53:58	arg1	potato					66:71	purple sweet potato	53:71	purple sweet potato	53:71	Regulatory effect of non-starch polysaccharides from purple sweet potato on intestinal microbiota of mice with antibiotic-associated diarrhea.
34008607	9	52	dep	composition	1384:1394	arg1	the					1380:1382	the	1380:1382	the	1380:1382	The results of 16S rRNA sequencing showed that PSPPs changed the composition and diversity of the intestinal flora of mice with AAD.
34008607	6	53	theme	pyran	926:930	arg1	polysaccharide					932:945	a pyran polysaccharide	924:945	a pyran polysaccharide with 1 → 2, 1 → 2, 6, 1 → 4, 1 → 4, 6 glycosidic bonds in an α-configuration	924:1022	The results showed that PSPP was a pyran polysaccharide with 1 → 2, 1 → 2, 6, 1 → 4, 1 → 4, 6 glycosidic bonds in an α-configuration.
34008607	6	53	theme	pyran	926:930	arg1	PSPP					915:918	PSPP	915:918	PSPP	915:918	The results showed that PSPP was a pyran polysaccharide with 1 → 2, 1 → 2, 6, 1 → 4, 1 → 4, 6 glycosidic bonds in an α-configuration.
34008607	6	54	with	polysaccharide	932:945	arg1	bonds					996:1000	1 → 2, 1 → 2, 6, 1 → 4, 1 → 4, 6 glycosidic bonds	952:1000	1 → 2, 1 → 2, 6, 1 → 4, 1 → 4, 6 glycosidic bonds in an α-configuration	952:1022	The results showed that PSPP was a pyran polysaccharide with 1 → 2, 1 → 2, 6, 1 → 4, 1 → 4, 6 glycosidic bonds in an α-configuration.
34008607	10	55	theme	acids	1523:1527	arg1	content					1494:1500	the content	1490:1500	the content of short-chain fatty acids (p < 0.01)	1490:1538	In addition, PSPP treatment increased the content of short-chain fatty acids (p < 0.01).
34008607	8	56	theme	mice	1302:1305	arg1	intestines					1288:1297	the intestines	1284:1297	the intestines of mice	1284:1305	In addition, treatment with PSPPs decreased the levels of IL-1β, IL-6 and TNF-α but increased the level of IL-10 in the intestines of mice (p < 0.01).
34008607	5	57	dep	g	839:839	arg1	kg-1					841:844	kg-1	841:844	kg-1	841:844	The diarrhea model was established via intragastric administration of lincomycin and different concentrations of PSPPs (0.1 g kg-1, 0.2 g kg-1, and 0.4 g kg-1) to Balb/C mice.
34008607	5	57	dep	g	839:839	arg1	kg-1					869:872	0.4 g kg-1	863:872	0.4 g kg-1	863:872	The diarrhea model was established via intragastric administration of lincomycin and different concentrations of PSPPs (0.1 g kg-1, 0.2 g kg-1, and 0.4 g kg-1) to Balb/C mice.
34008607	5	57	dep	g	839:839	arg1	kg-1					853:856	0.2 g kg-1	847:856	0.2 g kg-1	847:856	The diarrhea model was established via intragastric administration of lincomycin and different concentrations of PSPPs (0.1 g kg-1, 0.2 g kg-1, and 0.4 g kg-1) to Balb/C mice.
34008607	6	58	theme	2	956:956	arg1	bonds					996:1000	1 → 2, 1 → 2, 6, 1 → 4, 1 → 4, 6 glycosidic bonds	952:1000	1 → 2, 1 → 2, 6, 1 → 4, 1 → 4, 6 glycosidic bonds in an α-configuration	952:1022	The results showed that PSPP was a pyran polysaccharide with 1 → 2, 1 → 2, 6, 1 → 4, 1 → 4, 6 glycosidic bonds in an α-configuration.
34008607	1	59	theme	Antibiotic	143:152	arg1	treatment					154:162	Antibiotic treatment	143:162	Antibiotic treatment	143:162	Antibiotic treatment causes antibiotic-associated diarrhea (AAD), which is usually accompanied by disorders of the intestinal flora, aggravating the patient's condition.
34008607	5	60	theme	diarrhea	719:726	arg1	model					728:732	The diarrhea model	715:732	The diarrhea model	715:732	The diarrhea model was established via intragastric administration of lincomycin and different concentrations of PSPPs (0.1 g kg-1, 0.2 g kg-1, and 0.4 g kg-1) to Balb/C mice.
34008607	11	61	theme	intestinal	1589:1598	arg1	metabolism					1627:1636	balanced fatty acid metabolism	1607:1636	balanced fatty acid metabolism	1607:1636	These results revealed that PSPPs regulated the intestinal flora, balanced fatty acid metabolism, and relieved the symptoms of diarrhea to a certain extent in mice.
34008607	11	61	theme	intestinal	1589:1598	arg1	flora					1600:1604	the intestinal flora	1585:1604	the intestinal flora	1585:1604	These results revealed that PSPPs regulated the intestinal flora, balanced fatty acid metabolism, and relieved the symptoms of diarrhea to a certain extent in mice.
34008607	3	62	theme	sweet	480:484	arg1	PSPPs					510:514	PSPPs	510:514	PSPPs	510:514	However, reports on whether purple sweet potato polysaccharides (PSPPs) can improve AAD are scarce.
34008607	3	62	theme	sweet	480:484	arg1	polysaccharides					493:507	purple sweet potato polysaccharides	473:507	purple sweet potato polysaccharides (PSPPs)	473:515	However, reports on whether purple sweet potato polysaccharides (PSPPs) can improve AAD are scarce.
34008607	6	63	theme	glycosidic	985:994	arg1	bonds					996:1000	1 → 2, 1 → 2, 6, 1 → 4, 1 → 4, 6 glycosidic bonds	952:1000	1 → 2, 1 → 2, 6, 1 → 4, 1 → 4, 6 glycosidic bonds in an α-configuration	952:1022	The results showed that PSPP was a pyran polysaccharide with 1 → 2, 1 → 2, 6, 1 → 4, 1 → 4, 6 glycosidic bonds in an α-configuration.
34008607	3	64	theme	purple	473:478	arg1	PSPPs					510:514	PSPPs	510:514	PSPPs	510:514	However, reports on whether purple sweet potato polysaccharides (PSPPs) can improve AAD are scarce.
34008607	3	64	theme	purple	473:478	arg1	polysaccharides					493:507	purple sweet potato polysaccharides	473:507	purple sweet potato polysaccharides (PSPPs)	473:515	However, reports on whether purple sweet potato polysaccharides (PSPPs) can improve AAD are scarce.
34008607	9	65	with	mice	1437:1440	arg1	AAD					1447:1449	AAD	1447:1449	AAD	1447:1449	The results of 16S rRNA sequencing showed that PSPPs changed the composition and diversity of the intestinal flora of mice with AAD.
34008607	8	66	theme	p	1308:1308	arg1	<					1310:1310	p < 0.01	1308:1315	p < 0.01	1308:1315	In addition, treatment with PSPPs decreased the levels of IL-1β, IL-6 and TNF-α but increased the level of IL-10 in the intestines of mice (p < 0.01).
34008607	8	67	theme	IL-10	1275:1279	arg1	level					1266:1270	the level	1262:1270	the level of IL-10	1262:1279	In addition, treatment with PSPPs decreased the levels of IL-1β, IL-6 and TNF-α but increased the level of IL-10 in the intestines of mice (p < 0.01).
34008607	2	68	theme	plant	373:377	arg1	polysaccharides					379:393	plant polysaccharides	373:393	plant polysaccharides	373:393	Recently, more attention has been devoted to the ability of plant polysaccharides to improve the body's flora and enhance immunity.
34008607	11	69	theme	certain	1682:1688	arg1	extent					1690:1695	a certain extent	1680:1695	a certain extent in mice	1680:1703	These results revealed that PSPPs regulated the intestinal flora, balanced fatty acid metabolism, and relieved the symptoms of diarrhea to a certain extent in mice.
34008607	4	70	theme	sweet	613:617	arg1	potato					619:624	purple sweet potato	606:624	purple sweet potato	606:624	This study aimed to extract a non-starch polysaccharide from purple sweet potato and analyze its structure and ability to regulate the intestinal flora of mice with AAD.
34008607	5	71	theme	lincomycin	785:794	arg1	administration					767:780	intragastric administration	754:780	intragastric administration of lincomycin and different concentrations of PSPPs (0.1 g kg-1, 0.2 g kg-1, and 0.4 g kg-1) to Balb/C mice	754:888	The diarrhea model was established via intragastric administration of lincomycin and different concentrations of PSPPs (0.1 g kg-1, 0.2 g kg-1, and 0.4 g kg-1) to Balb/C mice.
34008607	4	72	theme	purple	606:611	arg1	potato					619:624	purple sweet potato	606:624	purple sweet potato	606:624	This study aimed to extract a non-starch polysaccharide from purple sweet potato and analyze its structure and ability to regulate the intestinal flora of mice with AAD.
34008607	1	73	theme	antibiotic-associated	171:191	arg1	AAD					203:205	AAD	203:205	AAD	203:205	Antibiotic treatment causes antibiotic-associated diarrhea (AAD), which is usually accompanied by disorders of the intestinal flora, aggravating the patient's condition.
34008607	1	73	theme	antibiotic-associated	171:191	arg1	diarrhea					193:200	antibiotic-associated diarrhea	171:200	antibiotic-associated diarrhea (AAD)	171:206	Antibiotic treatment causes antibiotic-associated diarrhea (AAD), which is usually accompanied by disorders of the intestinal flora, aggravating the patient's condition.
34008607	11	74	theme	acid	1622:1625	arg1	metabolism					1627:1636	balanced fatty acid metabolism	1607:1636	balanced fatty acid metabolism	1607:1636	These results revealed that PSPPs regulated the intestinal flora, balanced fatty acid metabolism, and relieved the symptoms of diarrhea to a certain extent in mice.
34008607	11	74	theme	acid	1622:1625	arg1	flora					1600:1604	the intestinal flora	1585:1604	the intestinal flora	1585:1604	These results revealed that PSPPs regulated the intestinal flora, balanced fatty acid metabolism, and relieved the symptoms of diarrhea to a certain extent in mice.
34008607	1	75	theme	intestinal	258:267	arg1	flora					269:273	the intestinal flora	254:273	the intestinal flora	254:273	Antibiotic treatment causes antibiotic-associated diarrhea (AAD), which is usually accompanied by disorders of the intestinal flora, aggravating the patient's condition.
34419545	0	0	theme	sweet	83:87	arg1	cultivars					114:122	five sweet potato (Ipomoea batatas) cultivars	78:122	five sweet potato (Ipomoea batatas) cultivars	78:122	Comparative study on physicochemical properties of starch films prepared from five sweet potato (Ipomoea batatas) cultivars.
34419545	1	1	theme	potato	277:282	arg1	starch					284:289	extract sweet potato starch	263:289	extract sweet potato starch (SPS) for developing starch-based films	263:329	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	1	theme	potato	277:282	arg1	SPS					292:294	SPS	292:294	SPS	292:294	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	2	theme	potato	146:151	arg1	Singeonmi					219:227	Singeonmi	219:227	Singeonmi	219:227	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	2	theme	potato	146:151	arg1	Sinyulmi					234:241	Sinyulmi	234:241	Sinyulmi	234:241	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	2	theme	potato	146:151	arg1	cultivars					171:179	Five different sweet potato (Ipomoea batatas) cultivars	125:179	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM])	125:248	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	2	theme	potato	146:151	arg1	Sincheonmi					201:210	Sincheonmi	201:210	Sincheonmi	201:210	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	2	theme	potato	146:151	arg1	Gogeonmi					191:198	Gogeonmi	191:198	Gogeonmi	191:198	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	3	3	theme	amylose	629:635	arg1	content					637:643	the highest amylose content	617:643	the highest amylose content (26.34%)	617:652	As one of the film characteristics, the X-ray diffractograms revealed that the SCM-based film with the highest amylose content (26.34%) had the highest relative crystallinity (24.31%).
34419545	3	3	theme	amylose	629:635	arg1	%					651:651	26.34%	646:651	26.34%	646:651	As one of the film characteristics, the X-ray diffractograms revealed that the SCM-based film with the highest amylose content (26.34%) had the highest relative crystallinity (24.31%).
34419545	0	4	from	study	12:16	arg1	properties					37:46	physicochemical properties	21:46	physicochemical properties of starch films prepared from five sweet potato (Ipomoea batatas) cultivars	21:122	Comparative study on physicochemical properties of starch films prepared from five sweet potato (Ipomoea batatas) cultivars.
34419545	2	5	theme	morphological	416:428	arg1	moisture					431:438	the morphological, moisture, mechanical, and barrier properties	412:474	moisture	431:438	After the chemical composition and amylose contents of all SPSs were evaluated, the morphological, moisture, mechanical, and barrier properties of the SPS-based films were investigated.
34419545	5	6	theme	water	872:876	arg1	vapor					878:882	The water vapor and oxygen permeabilities	868:908	vapor	878:882	The water vapor and oxygen permeabilities of the SPS-based films were negatively correlated with the amylose content.
34419545	4	7	theme	elastic	774:780	arg1	2.38-fold					791:799	2.38-fold	791:799	2.38-fold	791:799	The SCM-based film also showed higher tensile strength (3.05-fold) and elastic modulus (2.38-fold) than the SYM-based film with the lowest amylose content (21.84%).
34419545	4	7	theme	elastic	774:780	arg1	modulus					782:788	elastic modulus	774:788	elastic modulus (2.38-fold)	774:800	The SCM-based film also showed higher tensile strength (3.05-fold) and elastic modulus (2.38-fold) than the SYM-based film with the lowest amylose content (21.84%).
34419545	7	8	theme	significant	1205:1215	arg1	role					1217:1220	a significant role	1203:1220	a significant role	1203:1220	These results demonstrated that the sweet potato cultivar, especially the amylose content, plays a significant role in determining the physicochemical properties of the SPS-based films.
34419545	7	9	theme	films	1285:1289	arg1	properties					1257:1266	the physicochemical properties	1237:1266	the physicochemical properties of the SPS-based films	1237:1289	These results demonstrated that the sweet potato cultivar, especially the amylose content, plays a significant role in determining the physicochemical properties of the SPS-based films.
34419545	0	10	theme	potato	89:94	arg1	cultivars					114:122	five sweet potato (Ipomoea batatas) cultivars	78:122	five sweet potato (Ipomoea batatas) cultivars	78:122	Comparative study on physicochemical properties of starch films prepared from five sweet potato (Ipomoea batatas) cultivars.
34419545	0	11	theme	batatas	105:111	arg1	cultivars					114:122	five sweet potato (Ipomoea batatas) cultivars	78:122	five sweet potato (Ipomoea batatas) cultivars	78:122	Comparative study on physicochemical properties of starch films prepared from five sweet potato (Ipomoea batatas) cultivars.
34419545	5	12	theme	amylose	969:975	arg1	content					977:983	the amylose content	965:983	the amylose content	965:983	The water vapor and oxygen permeabilities of the SPS-based films were negatively correlated with the amylose content.
34419545	1	13	used	used	255:258	arg2	Gogeonmi					191:198	Gogeonmi	191:198	Gogeonmi	191:198	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	13	used	used	255:258	arg2	Sinyulmi					234:241	Sinyulmi	234:241	Sinyulmi	234:241	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	13	used	used	255:258	arg2	Singeonmi					219:227	Singeonmi	219:227	Singeonmi	219:227	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	13	used	used	255:258	arg2	cultivars					171:179	Five different sweet potato (Ipomoea batatas) cultivars	125:179	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM])	125:248	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	13	used	used	255:258	arg2	Sincheonmi					201:210	Sincheonmi	201:210	Sincheonmi	201:210	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	6	14	theme	water	1034:1038	arg1	3.16-fold					1047:1055	3.16-fold	1047:1055	3.16-fold	1047:1055	Thus, the SCM-based film was less permeable for water vapor (3.16-fold) and oxygen (1.81-fold) than the SYM-based film.
34419545	6	14	theme	water	1034:1038	arg1	vapor					1040:1044	water vapor	1034:1044	water vapor (3.16-fold)	1034:1056	Thus, the SCM-based film was less permeable for water vapor (3.16-fold) and oxygen (1.81-fold) than the SYM-based film.
34419545	1	15	theme	Ipomoea	154:160	arg1	Singeonmi					219:227	Singeonmi	219:227	Singeonmi	219:227	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	15	theme	Ipomoea	154:160	arg1	Sinyulmi					234:241	Sinyulmi	234:241	Sinyulmi	234:241	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	15	theme	Ipomoea	154:160	arg1	cultivars					171:179	Five different sweet potato (Ipomoea batatas) cultivars	125:179	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM])	125:248	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	15	theme	Ipomoea	154:160	arg1	Sincheonmi					201:210	Sincheonmi	201:210	Sincheonmi	201:210	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	15	theme	Ipomoea	154:160	arg1	Gogeonmi					191:198	Gogeonmi	191:198	Gogeonmi	191:198	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	0	16	theme	Ipomoea	97:103	arg1	cultivars					114:122	five sweet potato (Ipomoea batatas) cultivars	78:122	five sweet potato (Ipomoea batatas) cultivars	78:122	Comparative study on physicochemical properties of starch films prepared from five sweet potato (Ipomoea batatas) cultivars.
34419545	4	17	theme	higher	734:739	arg1	strength					749:756	higher tensile strength	734:756	higher tensile strength (3.05-fold)	734:768	The SCM-based film also showed higher tensile strength (3.05-fold) and elastic modulus (2.38-fold) than the SYM-based film with the lowest amylose content (21.84%).
34419545	4	17	theme	higher	734:739	arg1	3.05-fold					759:767	3.05-fold	759:767	3.05-fold	759:767	The SCM-based film also showed higher tensile strength (3.05-fold) and elastic modulus (2.38-fold) than the SYM-based film with the lowest amylose content (21.84%).
34419545	7	18	theme	SPS-based	1275:1283	arg1	films					1285:1289	the SPS-based films	1271:1289	the SPS-based films	1271:1289	These results demonstrated that the sweet potato cultivar, especially the amylose content, plays a significant role in determining the physicochemical properties of the SPS-based films.
34419545	1	19	theme	batatas	162:168	arg1	Singeonmi					219:227	Singeonmi	219:227	Singeonmi	219:227	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	19	theme	batatas	162:168	arg1	Sinyulmi					234:241	Sinyulmi	234:241	Sinyulmi	234:241	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	19	theme	batatas	162:168	arg1	cultivars					171:179	Five different sweet potato (Ipomoea batatas) cultivars	125:179	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM])	125:248	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	19	theme	batatas	162:168	arg1	Sincheonmi					201:210	Sincheonmi	201:210	Sincheonmi	201:210	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	19	theme	batatas	162:168	arg1	Gogeonmi					191:198	Gogeonmi	191:198	Gogeonmi	191:198	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	6	20	theme	SYM-based	1090:1098	arg1	film					1100:1103	the SYM-based film	1086:1103	the SYM-based film	1086:1103	Thus, the SCM-based film was less permeable for water vapor (3.16-fold) and oxygen (1.81-fold) than the SYM-based film.
34419545	3	21	contain	had	654:656	arg2	%					699:699	24.31%	694:699	24.31%	694:699	As one of the film characteristics, the X-ray diffractograms revealed that the SCM-based film with the highest amylose content (26.34%) had the highest relative crystallinity (24.31%).
34419545	3	21	contain	had	654:656	arg2	crystallinity					679:691	the highest relative crystallinity	658:691	the highest relative crystallinity (24.31%)	658:700	As one of the film characteristics, the X-ray diffractograms revealed that the SCM-based film with the highest amylose content (26.34%) had the highest relative crystallinity (24.31%).
34419545	3	21	contain	had	654:656	arg1	film					607:610	the SCM-based film	593:610	the SCM-based film with the highest amylose content (26.34%)	593:652	As one of the film characteristics, the X-ray diffractograms revealed that the SCM-based film with the highest amylose content (26.34%) had the highest relative crystallinity (24.31%).
34419545	3	22	with	film	607:610	arg1	content					637:643	the highest amylose content	617:643	the highest amylose content (26.34%)	617:652	As one of the film characteristics, the X-ray diffractograms revealed that the SCM-based film with the highest amylose content (26.34%) had the highest relative crystallinity (24.31%).
34419545	3	22	with	film	607:610	arg1	%					651:651	26.34%	646:651	26.34%	646:651	As one of the film characteristics, the X-ray diffractograms revealed that the SCM-based film with the highest amylose content (26.34%) had the highest relative crystallinity (24.31%).
34419545	0	23	theme	Comparative	0:10	arg1	study					12:16	Comparative study	0:16	Comparative study on physicochemical properties of starch films prepared from five sweet potato (Ipomoea batatas) cultivars.	0:123	Comparative study on physicochemical properties of starch films prepared from five sweet potato (Ipomoea batatas) cultivars.
34419545	3	24	theme	SCM-based	597:605	arg1	film					607:610	the SCM-based film	593:610	the SCM-based film with the highest amylose content (26.34%)	593:652	As one of the film characteristics, the X-ray diffractograms revealed that the SCM-based film with the highest amylose content (26.34%) had the highest relative crystallinity (24.31%).
34419545	6	25	dep	less	1015:1018	arg1	permeable					1020:1028	permeable	1020:1028	permeable	1020:1028	Thus, the SCM-based film was less permeable for water vapor (3.16-fold) and oxygen (1.81-fold) than the SYM-based film.
34419545	0	26	theme	physicochemical	21:35	arg1	properties					37:46	physicochemical properties	21:46	physicochemical properties of starch films prepared from five sweet potato (Ipomoea batatas) cultivars	21:122	Comparative study on physicochemical properties of starch films prepared from five sweet potato (Ipomoea batatas) cultivars.
34419545	3	27	theme	highest	662:668	arg1	crystallinity					679:691	the highest relative crystallinity	658:691	the highest relative crystallinity (24.31%)	658:700	As one of the film characteristics, the X-ray diffractograms revealed that the SCM-based film with the highest amylose content (26.34%) had the highest relative crystallinity (24.31%).
34419545	3	27	theme	highest	662:668	arg1	%					699:699	24.31%	694:699	24.31%	694:699	As one of the film characteristics, the X-ray diffractograms revealed that the SCM-based film with the highest amylose content (26.34%) had the highest relative crystallinity (24.31%).
34419545	7	28	theme	potato	1148:1153	arg1	content					1188:1194	the amylose content	1176:1194	especially the amylose content	1165:1194	These results demonstrated that the sweet potato cultivar, especially the amylose content, plays a significant role in determining the physicochemical properties of the SPS-based films.
34419545	7	28	theme	potato	1148:1153	arg1	cultivar					1155:1162	the sweet potato cultivar	1138:1162	the sweet potato cultivar	1138:1162	These results demonstrated that the sweet potato cultivar, especially the amylose content, plays a significant role in determining the physicochemical properties of the SPS-based films.
34419545	2	29	theme	amylose	367:373	arg1	contents					375:382	amylose contents	367:382	amylose contents	367:382	After the chemical composition and amylose contents of all SPSs were evaluated, the morphological, moisture, mechanical, and barrier properties of the SPS-based films were investigated.
34419545	1	30	theme	starch-based	312:323	arg1	films					325:329	starch-based films	312:329	starch-based films	312:329	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	7	31	theme	physicochemical	1241:1255	arg1	properties					1257:1266	the physicochemical properties	1237:1266	the physicochemical properties of the SPS-based films	1237:1289	These results demonstrated that the sweet potato cultivar, especially the amylose content, plays a significant role in determining the physicochemical properties of the SPS-based films.
34419545	7	32	theme	sweet	1142:1146	arg1	content					1188:1194	the amylose content	1176:1194	especially the amylose content	1165:1194	These results demonstrated that the sweet potato cultivar, especially the amylose content, plays a significant role in determining the physicochemical properties of the SPS-based films.
34419545	7	32	theme	sweet	1142:1146	arg1	cultivar					1155:1162	the sweet potato cultivar	1138:1162	the sweet potato cultivar	1138:1162	These results demonstrated that the sweet potato cultivar, especially the amylose content, plays a significant role in determining the physicochemical properties of the SPS-based films.
34419545	4	33	theme	tensile	741:747	arg1	strength					749:756	higher tensile strength	734:756	higher tensile strength (3.05-fold)	734:768	The SCM-based film also showed higher tensile strength (3.05-fold) and elastic modulus (2.38-fold) than the SYM-based film with the lowest amylose content (21.84%).
34419545	4	33	theme	tensile	741:747	arg1	3.05-fold					759:767	3.05-fold	759:767	3.05-fold	759:767	The SCM-based film also showed higher tensile strength (3.05-fold) and elastic modulus (2.38-fold) than the SYM-based film with the lowest amylose content (21.84%).
34419545	4	34	theme	SYM-based	811:819	arg1	film					821:824	the SYM-based film	807:824	the SYM-based film	807:824	The SCM-based film also showed higher tensile strength (3.05-fold) and elastic modulus (2.38-fold) than the SYM-based film with the lowest amylose content (21.84%).
34419545	2	35	theme	chemical	342:349	arg1	composition					351:361	chemical composition	342:361	chemical composition	342:361	After the chemical composition and amylose contents of all SPSs were evaluated, the morphological, moisture, mechanical, and barrier properties of the SPS-based films were investigated.
34419545	2	36	theme	barrier	457:463	arg1	properties					465:474	the morphological, moisture, mechanical, and barrier properties	412:474	properties	465:474	After the chemical composition and amylose contents of all SPSs were evaluated, the morphological, moisture, mechanical, and barrier properties of the SPS-based films were investigated.
34419545	1	37	dep	cultivars	171:179	arg1	Singeonmi					219:227	Singeonmi	219:227	Singeonmi	219:227	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	37	dep	cultivars	171:179	arg1	Sinyulmi					234:241	Sinyulmi	234:241	Sinyulmi	234:241	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	37	dep	cultivars	171:179	arg1	cultivars					171:179	Five different sweet potato (Ipomoea batatas) cultivars	125:179	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM])	125:248	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	37	dep	cultivars	171:179	arg1	Sincheonmi					201:210	Sincheonmi	201:210	Sincheonmi	201:210	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	37	dep	cultivars	171:179	arg1	Gogeonmi					191:198	Gogeonmi	191:198	Gogeonmi	191:198	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	4	38	theme	lowest	835:840	arg1	content					850:856	the lowest amylose content	831:856	the lowest amylose content (21.84%)	831:865	The SCM-based film also showed higher tensile strength (3.05-fold) and elastic modulus (2.38-fold) than the SYM-based film with the lowest amylose content (21.84%).
34419545	4	38	theme	lowest	835:840	arg1	%					864:864	21.84%	859:864	21.84%	859:864	The SCM-based film also showed higher tensile strength (3.05-fold) and elastic modulus (2.38-fold) than the SYM-based film with the lowest amylose content (21.84%).
34419545	0	39	theme	films	58:62	arg1	properties					37:46	physicochemical properties	21:46	physicochemical properties of starch films prepared from five sweet potato (Ipomoea batatas) cultivars	21:122	Comparative study on physicochemical properties of starch films prepared from five sweet potato (Ipomoea batatas) cultivars.
34419545	3	40	theme	X-ray	558:562	arg1	diffractograms					564:577	the X-ray diffractograms	554:577	the X-ray diffractograms	554:577	As one of the film characteristics, the X-ray diffractograms revealed that the SCM-based film with the highest amylose content (26.34%) had the highest relative crystallinity (24.31%).
34419545	5	41	theme	oxygen	888:893	arg1	permeabilities					895:908	The water vapor and oxygen permeabilities	868:908	permeabilities	895:908	The water vapor and oxygen permeabilities of the SPS-based films were negatively correlated with the amylose content.
34419545	6	42	theme	SCM-based	996:1004	arg1	less					1015:1018	less	1015:1018	less	1015:1018	Thus, the SCM-based film was less permeable for water vapor (3.16-fold) and oxygen (1.81-fold) than the SYM-based film.
34419545	6	42	theme	SCM-based	996:1004	arg1	film					1006:1009	the SCM-based film	992:1009	the SCM-based film	992:1009	Thus, the SCM-based film was less permeable for water vapor (3.16-fold) and oxygen (1.81-fold) than the SYM-based film.
34419545	0	43	theme	starch	51:56	arg1	films					58:62	starch films	51:62	starch films prepared from five sweet potato (Ipomoea batatas) cultivars	51:122	Comparative study on physicochemical properties of starch films prepared from five sweet potato (Ipomoea batatas) cultivars.
34419545	3	44	theme	relative	670:677	arg1	crystallinity					679:691	the highest relative crystallinity	658:691	the highest relative crystallinity (24.31%)	658:700	As one of the film characteristics, the X-ray diffractograms revealed that the SCM-based film with the highest amylose content (26.34%) had the highest relative crystallinity (24.31%).
34419545	3	44	theme	relative	670:677	arg1	%					699:699	24.31%	694:699	24.31%	694:699	As one of the film characteristics, the X-ray diffractograms revealed that the SCM-based film with the highest amylose content (26.34%) had the highest relative crystallinity (24.31%).
34419545	3	45	theme	highest	621:627	arg1	content					637:643	the highest amylose content	617:643	the highest amylose content (26.34%)	617:652	As one of the film characteristics, the X-ray diffractograms revealed that the SCM-based film with the highest amylose content (26.34%) had the highest relative crystallinity (24.31%).
34419545	3	45	theme	highest	621:627	arg1	%					651:651	26.34%	646:651	26.34%	646:651	As one of the film characteristics, the X-ray diffractograms revealed that the SCM-based film with the highest amylose content (26.34%) had the highest relative crystallinity (24.31%).
34419545	2	46	theme	SPSs	391:394	arg1	composition					351:361	chemical composition	342:361	chemical composition	342:361	After the chemical composition and amylose contents of all SPSs were evaluated, the morphological, moisture, mechanical, and barrier properties of the SPS-based films were investigated.
34419545	2	46	theme	SPSs	391:394	arg1	contents					375:382	amylose contents	367:382	amylose contents	367:382	After the chemical composition and amylose contents of all SPSs were evaluated, the morphological, moisture, mechanical, and barrier properties of the SPS-based films were investigated.
34419545	2	47	dep	composition	351:361	arg1	the					338:340	the	338:340	the	338:340	After the chemical composition and amylose contents of all SPSs were evaluated, the morphological, moisture, mechanical, and barrier properties of the SPS-based films were investigated.
34419545	4	48	theme	amylose	842:848	arg1	content					850:856	the lowest amylose content	831:856	the lowest amylose content (21.84%)	831:865	The SCM-based film also showed higher tensile strength (3.05-fold) and elastic modulus (2.38-fold) than the SYM-based film with the lowest amylose content (21.84%).
34419545	4	48	theme	amylose	842:848	arg1	%					864:864	21.84%	859:864	21.84%	859:864	The SCM-based film also showed higher tensile strength (3.05-fold) and elastic modulus (2.38-fold) than the SYM-based film with the lowest amylose content (21.84%).
34419545	1	49	theme	extract	263:269	arg1	starch					284:289	extract sweet potato starch	263:289	extract sweet potato starch (SPS) for developing starch-based films	263:329	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	49	theme	extract	263:269	arg1	SPS					292:294	SPS	292:294	SPS	292:294	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	2	50	theme	films	493:497	arg1	moisture					431:438	the morphological, moisture, mechanical, and barrier properties	412:474	moisture	431:438	After the chemical composition and amylose contents of all SPSs were evaluated, the morphological, moisture, mechanical, and barrier properties of the SPS-based films were investigated.
34419545	2	50	theme	films	493:497	arg1	properties					465:474	the morphological, moisture, mechanical, and barrier properties	412:474	properties	465:474	After the chemical composition and amylose contents of all SPSs were evaluated, the morphological, moisture, mechanical, and barrier properties of the SPS-based films were investigated.
34419545	3	51	theme	characteristics	537:551	arg1	one					521:523	one	521:523	one	521:523	As one of the film characteristics, the X-ray diffractograms revealed that the SCM-based film with the highest amylose content (26.34%) had the highest relative crystallinity (24.31%).
34419545	3	51	theme	characteristics	537:551	arg1	characteristics					537:551	the film characteristics	528:551	the film characteristics	528:551	As one of the film characteristics, the X-ray diffractograms revealed that the SCM-based film with the highest amylose content (26.34%) had the highest relative crystallinity (24.31%).
34419545	4	52	theme	SCM-based	707:715	arg1	film					717:720	The SCM-based film	703:720	The SCM-based film	703:720	The SCM-based film also showed higher tensile strength (3.05-fold) and elastic modulus (2.38-fold) than the SYM-based film with the lowest amylose content (21.84%).
34419545	2	53	theme	SPS-based	483:491	arg1	films					493:497	the SPS-based films	479:497	the SPS-based films	479:497	After the chemical composition and amylose contents of all SPSs were evaluated, the morphological, moisture, mechanical, and barrier properties of the SPS-based films were investigated.
34419545	5	54	theme	SPS-based	917:925	arg1	films					927:931	the SPS-based films	913:931	the SPS-based films	913:931	The water vapor and oxygen permeabilities of the SPS-based films were negatively correlated with the amylose content.
34419545	2	55	theme	mechanical	441:450	arg1	moisture					431:438	the morphological, moisture, mechanical, and barrier properties	412:474	moisture	431:438	After the chemical composition and amylose contents of all SPSs were evaluated, the morphological, moisture, mechanical, and barrier properties of the SPS-based films were investigated.
34419545	5	56	theme	films	927:931	arg1	vapor					878:882	The water vapor and oxygen permeabilities	868:908	vapor	878:882	The water vapor and oxygen permeabilities of the SPS-based films were negatively correlated with the amylose content.
34419545	5	56	theme	films	927:931	arg1	permeabilities					895:908	The water vapor and oxygen permeabilities	868:908	permeabilities	895:908	The water vapor and oxygen permeabilities of the SPS-based films were negatively correlated with the amylose content.
34419545	1	57	theme	different	130:138	arg1	Singeonmi					219:227	Singeonmi	219:227	Singeonmi	219:227	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	57	theme	different	130:138	arg1	Sinyulmi					234:241	Sinyulmi	234:241	Sinyulmi	234:241	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	57	theme	different	130:138	arg1	cultivars					171:179	Five different sweet potato (Ipomoea batatas) cultivars	125:179	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM])	125:248	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	57	theme	different	130:138	arg1	Sincheonmi					201:210	Sincheonmi	201:210	Sincheonmi	201:210	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	57	theme	different	130:138	arg1	Gogeonmi					191:198	Gogeonmi	191:198	Gogeonmi	191:198	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	7	58	theme	amylose	1180:1186	arg1	content					1188:1194	the amylose content	1176:1194	especially the amylose content	1165:1194	These results demonstrated that the sweet potato cultivar, especially the amylose content, plays a significant role in determining the physicochemical properties of the SPS-based films.
34419545	7	58	theme	amylose	1180:1186	arg1	cultivar					1155:1162	the sweet potato cultivar	1138:1162	the sweet potato cultivar	1138:1162	These results demonstrated that the sweet potato cultivar, especially the amylose content, plays a significant role in determining the physicochemical properties of the SPS-based films.
34419545	1	59	theme	sweet	271:275	arg1	starch					284:289	extract sweet potato starch	263:289	extract sweet potato starch (SPS) for developing starch-based films	263:329	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	59	theme	sweet	271:275	arg1	SPS					292:294	SPS	292:294	SPS	292:294	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	3	60	theme	film	532:535	arg1	characteristics					537:551	the film characteristics	528:551	the film characteristics	528:551	As one of the film characteristics, the X-ray diffractograms revealed that the SCM-based film with the highest amylose content (26.34%) had the highest relative crystallinity (24.31%).
34419545	1	61	theme	sweet	140:144	arg1	Singeonmi					219:227	Singeonmi	219:227	Singeonmi	219:227	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	61	theme	sweet	140:144	arg1	Sinyulmi					234:241	Sinyulmi	234:241	Sinyulmi	234:241	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	61	theme	sweet	140:144	arg1	cultivars					171:179	Five different sweet potato (Ipomoea batatas) cultivars	125:179	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM])	125:248	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	61	theme	sweet	140:144	arg1	Sincheonmi					201:210	Sincheonmi	201:210	Sincheonmi	201:210	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34419545	1	61	theme	sweet	140:144	arg1	Gogeonmi					191:198	Gogeonmi	191:198	Gogeonmi	191:198	Five different sweet potato (Ipomoea batatas) cultivars (Daeyumi, Gogeonmi, Sincheonmi [SCM], Singeonmi, and Sinyulmi [SYM]) were used to extract sweet potato starch (SPS) for developing starch-based films.
34157519	13	0	theme	immune	1922:1927	arg1	cells					1929:1933	immune cells	1922:1933	immune cells	1922:1933	ALG-CS-CHY scaffolds also increased collagen deposition, hexosamine synthesis, accelerates angiogenesis, and recruiting immune cells at the site of a wound.
34157519	9	1	theme	minimized	1154:1162	arg1	re-application					1164:1177	minimized re-application	1154:1177	minimized re-application	1154:1177	The ALG-CS scaffold showed sustained drug availability and minimized re-application, which contributes to effective healing and treatment.
34157519	11	2	theme	Calcein	1359:1365	arg1	AM					1367:1368	Calcein AM	1359:1368	Calcein AM	1359:1368	Calcein AM, Propidium iodide, was used for live and dead cell staining, which confirmed that fabricated ALG-CS-CHY scaffolds are biocompatible and facilitate cell growth and cell proliferation.
34157519	11	2	theme	Calcein	1359:1365	arg1	iodide					1381:1386	Propidium iodide	1371:1386	Propidium iodide	1371:1386	Calcein AM, Propidium iodide, was used for live and dead cell staining, which confirmed that fabricated ALG-CS-CHY scaffolds are biocompatible and facilitate cell growth and cell proliferation.
34157519	12	3	dep	significantly	1750:1762	arg1	p					1765:1765	p < 0.001	1765:1773	p < 0.001	1765:1773	In-vivo and in-vitro observations show that the experimental group treated using the ALG-CS-CHY reduces the period of re-epithelization, accelerated fibroblast cell migration, and contracted wound significantly (p < 0.001) compared to other groups.
34157519	1	4	theme	dermal	259:264	arg1	management					272:281	dermal wound management	259:281	dermal wound management	259:281	The present study explores the therapeutic efficacy of sodium alginate-chitosan scaffolds loaded with Chrysin (ALG-CS-CHY) for dermal wound management.
34157519	0	5	theme	alginate	72:79	arg1	-chitosan					81:89	alginate -chitosan	72:89	alginate -chitosan	72:89	Fabrication and characterization of Chrysin - A plant polyphenol loaded alginate -chitosan composite for wound healing application.
34157519	8	6	theme	high	1070:1073	arg1	degree					1075:1080	a high degree	1068:1080	a high degree of porosity	1068:1092	The swelling ratio was highest in the first hour and decreased slowly with time, and the porosity analysis showed a high degree of porosity.
34157519	5	7	theme	X-ray	621:625	arg1	Diffraction					627:637	X-ray Diffraction	621:637	X-ray Diffraction (XRD)	621:643	X-ray Diffraction (XRD) showed an ionic interaction between ALG-CS leading to their excellent compatibility.
34157519	5	7	theme	X-ray	621:625	arg1	XRD					640:642	XRD	640:642	XRD	640:642	X-ray Diffraction (XRD) showed an ionic interaction between ALG-CS leading to their excellent compatibility.
34157519	1	8	theme	therapeutic	163:173	arg1	efficacy					175:182	the therapeutic efficacy	159:182	the therapeutic efficacy of sodium alginate-chitosan scaffolds loaded with Chrysin (ALG-CS-CHY) for dermal wound management	159:281	The present study explores the therapeutic efficacy of sodium alginate-chitosan scaffolds loaded with Chrysin (ALG-CS-CHY) for dermal wound management.
34157519	9	9	theme	ALG-CS	1099:1104	arg1	scaffold					1106:1113	The ALG-CS scaffold	1095:1113	The ALG-CS scaffold	1095:1113	The ALG-CS scaffold showed sustained drug availability and minimized re-application, which contributes to effective healing and treatment.
34157519	1	10	theme	wound	266:270	arg1	management					272:281	dermal wound management	259:281	dermal wound management	259:281	The present study explores the therapeutic efficacy of sodium alginate-chitosan scaffolds loaded with Chrysin (ALG-CS-CHY) for dermal wound management.
34157519	4	11	theme	Electron	541:548	arg1	SEM					562:564	SEM	562:564	SEM	562:564	Surface Electron microscopy (SEM) showed a porous architecture varying from 200-400 μm.
34157519	4	11	theme	Electron	541:548	arg1	microscopy					550:559	Surface Electron microscopy	533:559	Surface Electron microscopy (SEM)	533:565	Surface Electron microscopy (SEM) showed a porous architecture varying from 200-400 μm.
34157519	8	12	theme	porosity	1085:1092	arg1	degree					1075:1080	a high degree	1068:1080	a high degree of porosity	1068:1092	The swelling ratio was highest in the first hour and decreased slowly with time, and the porosity analysis showed a high degree of porosity.
34157519	8	13	theme	first	992:996	arg1	hour					998:1001	the first hour	988:1001	the first hour	988:1001	The swelling ratio was highest in the first hour and decreased slowly with time, and the porosity analysis showed a high degree of porosity.
34157519	6	14	theme	Calorimetry	752:762	arg1	results					807:813	Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) results	730:813	Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) results	730:813	Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) results suggest increased ALG-CS scaffold's thermal stability.
34157519	10	15	theme	whole	1262:1266	arg1	blood					1268:1272	whole blood	1262:1272	whole blood	1262:1272	The blood compatibility and whole blood clotting ability of the scaffold significantly improved after incorporating the drug.
34157519	0	16	theme	healing	111:117	arg1	application					119:129	wound healing application	105:129	wound healing application	105:129	Fabrication and characterization of Chrysin - A plant polyphenol loaded alginate -chitosan composite for wound healing application.
34157519	7	17	theme	controlled	916:925	arg1	degradation					927:937	controlled degradation	916:937	controlled degradation with lysozyme	916:951	In-vitro biodegradation behavior demonstrated controlled degradation with lysozyme.
34157519	6	18	theme	ALG-CS	833:838	arg1	stability					859:867	increased ALG-CS scaffold's thermal stability	823:867	increased ALG-CS scaffold's thermal stability	823:867	Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) results suggest increased ALG-CS scaffold's thermal stability.
34157519	6	19	theme	Scanning	743:750	arg1	DSC					765:767	DSC	765:767	DSC	765:767	Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) results suggest increased ALG-CS scaffold's thermal stability.
34157519	6	19	theme	Scanning	743:750	arg1	Calorimetry					752:762	Differential Scanning Calorimetry	730:762	Differential Scanning Calorimetry (DSC)	730:768	Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) results suggest increased ALG-CS scaffold's thermal stability.
34157519	14	20	theme	effective	2014:2022	arg1	dressing					2024:2031	an effective dressing	2011:2031	an effective dressing for dermal wound management	2011:2059	These results suggest ALG-CS-CHY scaffold serves as an effective dressing for dermal wound management.
34157519	0	21	theme	wound	105:109	arg1	healing					111:117	wound healing	105:117	wound healing application	105:129	Fabrication and characterization of Chrysin - A plant polyphenol loaded alginate -chitosan composite for wound healing application.
34157519	6	22	theme	increased	823:831	arg1	stability					859:867	increased ALG-CS scaffold's thermal stability	823:867	increased ALG-CS scaffold's thermal stability	823:867	Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) results suggest increased ALG-CS scaffold's thermal stability.
34157519	12	23	theme	fibroblast	1702:1711	arg1	migration					1718:1726	accelerated fibroblast cell migration	1690:1726	accelerated fibroblast cell migration	1690:1726	In-vivo and in-vitro observations show that the experimental group treated using the ALG-CS-CHY reduces the period of re-epithelization, accelerated fibroblast cell migration, and contracted wound significantly (p < 0.001) compared to other groups.
34157519	6	24	theme	Differential	730:741	arg1	DSC					765:767	DSC	765:767	DSC	765:767	Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) results suggest increased ALG-CS scaffold's thermal stability.
34157519	6	24	theme	Differential	730:741	arg1	Calorimetry					752:762	Differential Scanning Calorimetry	730:762	Differential Scanning Calorimetry (DSC)	730:768	Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) results suggest increased ALG-CS scaffold's thermal stability.
34157519	11	25	theme	cell	1517:1520	arg1	growth					1522:1527	cell growth	1517:1527	cell growth	1517:1527	Calcein AM, Propidium iodide, was used for live and dead cell staining, which confirmed that fabricated ALG-CS-CHY scaffolds are biocompatible and facilitate cell growth and cell proliferation.
34157519	12	26	theme	<	1767:1767	arg1	p					1765:1765	p < 0.001	1765:1773	p < 0.001	1765:1773	In-vivo and in-vitro observations show that the experimental group treated using the ALG-CS-CHY reduces the period of re-epithelization, accelerated fibroblast cell migration, and contracted wound significantly (p < 0.001) compared to other groups.
34157519	12	27	theme	cell	1713:1716	arg1	migration					1718:1726	accelerated fibroblast cell migration	1690:1726	accelerated fibroblast cell migration	1690:1726	In-vivo and in-vitro observations show that the experimental group treated using the ALG-CS-CHY reduces the period of re-epithelization, accelerated fibroblast cell migration, and contracted wound significantly (p < 0.001) compared to other groups.
34157519	8	28	theme	porosity	1043:1050	arg1	analysis					1052:1059	the porosity analysis	1039:1059	the porosity analysis	1039:1059	The swelling ratio was highest in the first hour and decreased slowly with time, and the porosity analysis showed a high degree of porosity.
34157519	1	29	theme	sodium	187:192	arg1	scaffolds					212:220	sodium alginate-chitosan scaffolds	187:220	sodium alginate-chitosan scaffolds loaded with Chrysin (ALG-CS-CHY)	187:253	The present study explores the therapeutic efficacy of sodium alginate-chitosan scaffolds loaded with Chrysin (ALG-CS-CHY) for dermal wound management.
34157519	11	30	theme	live	1402:1405	arg1	staining					1421:1428	live and dead cell staining	1402:1428	live and dead cell staining	1402:1428	Calcein AM, Propidium iodide, was used for live and dead cell staining, which confirmed that fabricated ALG-CS-CHY scaffolds are biocompatible and facilitate cell growth and cell proliferation.
34157519	4	31	theme	porous	576:581	arg1	architecture					583:594	a porous architecture	574:594	a porous architecture varying from 200-400 μm	574:618	Surface Electron microscopy (SEM) showed a porous architecture varying from 200-400 μm.
34157519	5	32	theme	ionic	655:659	arg1	interaction					661:671	an ionic interaction	652:671	an ionic interaction between ALG-CS leading to their excellent compatibility	652:727	X-ray Diffraction (XRD) showed an ionic interaction between ALG-CS leading to their excellent compatibility.
34157519	2	33	theme	freeze-drying	322:334	arg1	method					336:341	the vacuum freeze-drying method	311:341	the vacuum freeze-drying method	311:341	Scaffolds were prepared by the vacuum freeze-drying method.
34157519	7	34	theme	In-vitro	870:877	arg1	behavior					894:901	In-vitro biodegradation behavior	870:901	In-vitro biodegradation behavior	870:901	In-vitro biodegradation behavior demonstrated controlled degradation with lysozyme.
34157519	1	35	theme	alginate-chitosan	194:210	arg1	scaffolds					212:220	sodium alginate-chitosan scaffolds	187:220	sodium alginate-chitosan scaffolds loaded with Chrysin (ALG-CS-CHY)	187:253	The present study explores the therapeutic efficacy of sodium alginate-chitosan scaffolds loaded with Chrysin (ALG-CS-CHY) for dermal wound management.
34157519	13	36	theme	hexosamine	1859:1868	arg1	deposition					1847:1856	increased collagen deposition	1828:1856	increased collagen deposition	1828:1856	ALG-CS-CHY scaffolds also increased collagen deposition, hexosamine synthesis, accelerates angiogenesis, and recruiting immune cells at the site of a wound.
34157519	13	36	theme	hexosamine	1859:1868	arg1	synthesis					1870:1878	hexosamine synthesis	1859:1878	hexosamine synthesis	1859:1878	ALG-CS-CHY scaffolds also increased collagen deposition, hexosamine synthesis, accelerates angiogenesis, and recruiting immune cells at the site of a wound.
34157519	12	37	theme	accelerated	1690:1700	arg1	migration					1718:1726	accelerated fibroblast cell migration	1690:1726	accelerated fibroblast cell migration	1690:1726	In-vivo and in-vitro observations show that the experimental group treated using the ALG-CS-CHY reduces the period of re-epithelization, accelerated fibroblast cell migration, and contracted wound significantly (p < 0.001) compared to other groups.
34157519	2	38	theme	vacuum	315:320	arg1	method					336:341	the vacuum freeze-drying method	311:341	the vacuum freeze-drying method	311:341	Scaffolds were prepared by the vacuum freeze-drying method.
34157519	6	39	theme	thermal	851:857	arg1	stability					859:867	increased ALG-CS scaffold's thermal stability	823:867	increased ALG-CS scaffold's thermal stability	823:867	Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) results suggest increased ALG-CS scaffold's thermal stability.
34157519	1	40	theme	scaffolds	212:220	arg1	efficacy					175:182	the therapeutic efficacy	159:182	the therapeutic efficacy of sodium alginate-chitosan scaffolds loaded with Chrysin (ALG-CS-CHY) for dermal wound management	159:281	The present study explores the therapeutic efficacy of sodium alginate-chitosan scaffolds loaded with Chrysin (ALG-CS-CHY) for dermal wound management.
34157519	3	41	theme	Infra-Red	415:423	arg1	Spectroscopy					425:436	Infra-Red Spectroscopy	415:436	Infra-Red Spectroscopy (FTIR)	415:443	The physiochemical characterization was done through Fourier Transform Infra-Red Spectroscopy (FTIR), which revealed the interactions between the scaffold's functional groups and the drug.
34157519	3	41	theme	Infra-Red	415:423	arg1	FTIR					439:442	FTIR	439:442	FTIR	439:442	The physiochemical characterization was done through Fourier Transform Infra-Red Spectroscopy (FTIR), which revealed the interactions between the scaffold's functional groups and the drug.
34157519	12	42	theme	migration	1718:1726	arg1	period					1661:1666	the period	1657:1666	the period of re-epithelization, accelerated fibroblast cell migration, and contracted wound	1657:1748	In-vivo and in-vitro observations show that the experimental group treated using the ALG-CS-CHY reduces the period of re-epithelization, accelerated fibroblast cell migration, and contracted wound significantly (p < 0.001) compared to other groups.
34157519	12	43	theme	wound	1744:1748	arg1	period					1661:1666	the period	1657:1666	the period of re-epithelization, accelerated fibroblast cell migration, and contracted wound	1657:1748	In-vivo and in-vitro observations show that the experimental group treated using the ALG-CS-CHY reduces the period of re-epithelization, accelerated fibroblast cell migration, and contracted wound significantly (p < 0.001) compared to other groups.
34157519	3	44	theme	functional	501:510	arg1	groups					512:517	the scaffold's functional groups	486:517	the scaffold's functional groups	486:517	The physiochemical characterization was done through Fourier Transform Infra-Red Spectroscopy (FTIR), which revealed the interactions between the scaffold's functional groups and the drug.
34157519	11	45	theme	cell	1416:1419	arg1	staining					1421:1428	live and dead cell staining	1402:1428	live and dead cell staining	1402:1428	Calcein AM, Propidium iodide, was used for live and dead cell staining, which confirmed that fabricated ALG-CS-CHY scaffolds are biocompatible and facilitate cell growth and cell proliferation.
34157519	9	46	theme	sustained	1122:1130	arg1	availability					1137:1148	sustained drug availability	1122:1148	sustained drug availability	1122:1148	The ALG-CS scaffold showed sustained drug availability and minimized re-application, which contributes to effective healing and treatment.
34157519	12	47	theme	contracted	1733:1742	arg1	wound					1744:1748	contracted wound	1733:1748	contracted wound	1733:1748	In-vivo and in-vitro observations show that the experimental group treated using the ALG-CS-CHY reduces the period of re-epithelization, accelerated fibroblast cell migration, and contracted wound significantly (p < 0.001) compared to other groups.
34157519	9	48	theme	effective	1201:1209	arg1	healing					1211:1217	effective healing	1201:1217	effective healing	1201:1217	The ALG-CS scaffold showed sustained drug availability and minimized re-application, which contributes to effective healing and treatment.
34157519	0	49	theme	Chrysin	36:42	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of Chrysin - A plant polyphenol loaded alginate -chitosan composite for wound healing application.
34157519	0	49	theme	Chrysin	36:42	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of Chrysin - A plant polyphenol loaded alginate -chitosan composite for wound healing application.
34157519	12	50	theme	experimental	1601:1612	arg1	group					1614:1618	the experimental group	1597:1618	the experimental group treated using the ALG-CS-CHY	1597:1647	In-vivo and in-vitro observations show that the experimental group treated using the ALG-CS-CHY reduces the period of re-epithelization, accelerated fibroblast cell migration, and contracted wound significantly (p < 0.001) compared to other groups.
34157519	11	51	theme	ALG-CS-CHY	1463:1472	arg1	scaffolds					1474:1482	fabricated ALG-CS-CHY scaffolds	1452:1482	fabricated ALG-CS-CHY scaffolds	1452:1482	Calcein AM, Propidium iodide, was used for live and dead cell staining, which confirmed that fabricated ALG-CS-CHY scaffolds are biocompatible and facilitate cell growth and cell proliferation.
34157519	0	52	theme	plant	48:52	arg1	polyphenol					54:63	A plant polyphenol	46:63	Fabrication and characterization of Chrysin - A plant polyphenol loaded alginate -chitosan composite for wound healing application.	0:130	Fabrication and characterization of Chrysin - A plant polyphenol loaded alginate -chitosan composite for wound healing application.
34157519	7	53	theme	biodegradation	879:892	arg1	behavior					894:901	In-vitro biodegradation behavior	870:901	In-vitro biodegradation behavior	870:901	In-vitro biodegradation behavior demonstrated controlled degradation with lysozyme.
34157519	14	54	dep	suggest	1973:1979	arg1	serves					2001:2006	serves	2001:2006	suggest ALG-CS-CHY scaffold serves as an effective dressing for dermal wound management	1973:2059	These results suggest ALG-CS-CHY scaffold serves as an effective dressing for dermal wound management.
34157519	11	55	theme	dead	1411:1414	arg1	staining					1421:1428	live and dead cell staining	1402:1428	live and dead cell staining	1402:1428	Calcein AM, Propidium iodide, was used for live and dead cell staining, which confirmed that fabricated ALG-CS-CHY scaffolds are biocompatible and facilitate cell growth and cell proliferation.
34157519	11	56	used	used	1393:1396	arg2	iodide					1381:1386	Propidium iodide	1371:1386	Propidium iodide	1371:1386	Calcein AM, Propidium iodide, was used for live and dead cell staining, which confirmed that fabricated ALG-CS-CHY scaffolds are biocompatible and facilitate cell growth and cell proliferation.
34157519	11	56	used	used	1393:1396	arg2	AM					1367:1368	Calcein AM	1359:1368	Calcein AM	1359:1368	Calcein AM, Propidium iodide, was used for live and dead cell staining, which confirmed that fabricated ALG-CS-CHY scaffolds are biocompatible and facilitate cell growth and cell proliferation.
34157519	10	57	dep	blood	1238:1242	arg1	compatibility					1244:1256	compatibility	1244:1256	compatibility	1244:1256	The blood compatibility and whole blood clotting ability of the scaffold significantly improved after incorporating the drug.
34157519	13	58	theme	collagen	1838:1845	arg1	deposition					1847:1856	increased collagen deposition	1828:1856	increased collagen deposition	1828:1856	ALG-CS-CHY scaffolds also increased collagen deposition, hexosamine synthesis, accelerates angiogenesis, and recruiting immune cells at the site of a wound.
34157519	13	58	theme	collagen	1838:1845	arg1	synthesis					1870:1878	hexosamine synthesis	1859:1878	hexosamine synthesis	1859:1878	ALG-CS-CHY scaffolds also increased collagen deposition, hexosamine synthesis, accelerates angiogenesis, and recruiting immune cells at the site of a wound.
34157519	6	59	theme	Analysis	792:799	arg1	results					807:813	Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) results	730:813	Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) results	730:813	Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) results suggest increased ALG-CS scaffold's thermal stability.
34157519	12	60	theme	re-epithelization	1671:1687	arg1	period					1661:1666	the period	1657:1666	the period of re-epithelization, accelerated fibroblast cell migration, and contracted wound	1657:1748	In-vivo and in-vitro observations show that the experimental group treated using the ALG-CS-CHY reduces the period of re-epithelization, accelerated fibroblast cell migration, and contracted wound significantly (p < 0.001) compared to other groups.
34157519	5	61	theme	excellent	705:713	arg1	compatibility					715:727	their excellent compatibility	699:727	their excellent compatibility	699:727	X-ray Diffraction (XRD) showed an ionic interaction between ALG-CS leading to their excellent compatibility.
34157519	14	62	theme	wound	2044:2048	arg1	management					2050:2059	dermal wound management	2037:2059	dermal wound management	2037:2059	These results suggest ALG-CS-CHY scaffold serves as an effective dressing for dermal wound management.
34157519	6	63	theme	Thermogravimetric	774:790	arg1	Analysis					792:799	Thermogravimetric Analysis	774:799	Thermogravimetric Analysis (TGA)	774:805	Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) results suggest increased ALG-CS scaffold's thermal stability.
34157519	6	63	theme	Thermogravimetric	774:790	arg1	TGA					802:804	TGA	802:804	TGA	802:804	Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) results suggest increased ALG-CS scaffold's thermal stability.
34157519	13	64	theme	increased	1828:1836	arg1	deposition					1847:1856	increased collagen deposition	1828:1856	increased collagen deposition	1828:1856	ALG-CS-CHY scaffolds also increased collagen deposition, hexosamine synthesis, accelerates angiogenesis, and recruiting immune cells at the site of a wound.
34157519	13	64	theme	increased	1828:1836	arg1	synthesis					1870:1878	hexosamine synthesis	1859:1878	hexosamine synthesis	1859:1878	ALG-CS-CHY scaffolds also increased collagen deposition, hexosamine synthesis, accelerates angiogenesis, and recruiting immune cells at the site of a wound.
34157519	10	65	theme	scaffold	1298:1305	arg1	ability					1283:1289	ability	1283:1289	ability of the scaffold	1283:1305	The blood compatibility and whole blood clotting ability of the scaffold significantly improved after incorporating the drug.
34157519	3	66	dep	Fourier	397:403	arg1	Transform					405:413	Transform	405:413	Transform Infra-Red Spectroscopy (FTIR)	405:443	The physiochemical characterization was done through Fourier Transform Infra-Red Spectroscopy (FTIR), which revealed the interactions between the scaffold's functional groups and the drug.
34157519	12	67	theme	in-vitro	1565:1572	arg1	observations					1574:1585	in-vitro observations	1565:1585	in-vitro observations	1565:1585	In-vivo and in-vitro observations show that the experimental group treated using the ALG-CS-CHY reduces the period of re-epithelization, accelerated fibroblast cell migration, and contracted wound significantly (p < 0.001) compared to other groups.
34157519	14	68	theme	dermal	2037:2042	arg1	management					2050:2059	dermal wound management	2037:2059	dermal wound management	2037:2059	These results suggest ALG-CS-CHY scaffold serves as an effective dressing for dermal wound management.
34157519	14	69	theme	ALG-CS-CHY	1981:1990	arg1	scaffold					1992:1999	ALG-CS-CHY scaffold	1981:1999	ALG-CS-CHY scaffold	1981:1999	These results suggest ALG-CS-CHY scaffold serves as an effective dressing for dermal wound management.
34157519	1	70	theme	present	136:142	arg1	study					144:148	The present study	132:148	The present study	132:148	The present study explores the therapeutic efficacy of sodium alginate-chitosan scaffolds loaded with Chrysin (ALG-CS-CHY) for dermal wound management.
34157519	9	71	theme	drug	1132:1135	arg1	availability					1137:1148	sustained drug availability	1122:1148	sustained drug availability	1122:1148	The ALG-CS scaffold showed sustained drug availability and minimized re-application, which contributes to effective healing and treatment.
34157519	3	72	theme	physiochemical	348:361	arg1	characterization					363:378	The physiochemical characterization	344:378	The physiochemical characterization	344:378	The physiochemical characterization was done through Fourier Transform Infra-Red Spectroscopy (FTIR), which revealed the interactions between the scaffold's functional groups and the drug.
34157519	7	73	with	degradation	927:937	arg1	lysozyme					944:951	lysozyme	944:951	lysozyme	944:951	In-vitro biodegradation behavior demonstrated controlled degradation with lysozyme.
34157519	11	74	theme	Propidium	1371:1379	arg1	AM					1367:1368	Calcein AM	1359:1368	Calcein AM	1359:1368	Calcein AM, Propidium iodide, was used for live and dead cell staining, which confirmed that fabricated ALG-CS-CHY scaffolds are biocompatible and facilitate cell growth and cell proliferation.
34157519	11	74	theme	Propidium	1371:1379	arg1	iodide					1381:1386	Propidium iodide	1371:1386	Propidium iodide	1371:1386	Calcein AM, Propidium iodide, was used for live and dead cell staining, which confirmed that fabricated ALG-CS-CHY scaffolds are biocompatible and facilitate cell growth and cell proliferation.
34157519	13	75	theme	wound	1952:1956	arg1	site					1942:1945	the site	1938:1945	the site of a wound	1938:1956	ALG-CS-CHY scaffolds also increased collagen deposition, hexosamine synthesis, accelerates angiogenesis, and recruiting immune cells at the site of a wound.
34157519	13	75	theme	wound	1952:1956	arg1	wound					1952:1956	a wound	1950:1956	a wound	1950:1956	ALG-CS-CHY scaffolds also increased collagen deposition, hexosamine synthesis, accelerates angiogenesis, and recruiting immune cells at the site of a wound.
34157519	11	76	theme	cell	1533:1536	arg1	proliferation					1538:1550	cell proliferation	1533:1550	cell proliferation	1533:1550	Calcein AM, Propidium iodide, was used for live and dead cell staining, which confirmed that fabricated ALG-CS-CHY scaffolds are biocompatible and facilitate cell growth and cell proliferation.
34157519	8	77	theme	swelling	958:965	arg1	ratio					967:971	The swelling ratio	954:971	The swelling ratio	954:971	The swelling ratio was highest in the first hour and decreased slowly with time, and the porosity analysis showed a high degree of porosity.
34157519	8	77	theme	swelling	958:965	arg1	highest					977:983	highest	977:983	highest	977:983	The swelling ratio was highest in the first hour and decreased slowly with time, and the porosity analysis showed a high degree of porosity.
34157519	4	78	theme	Surface	533:539	arg1	SEM					562:564	SEM	562:564	SEM	562:564	Surface Electron microscopy (SEM) showed a porous architecture varying from 200-400 μm.
34157519	4	78	theme	Surface	533:539	arg1	microscopy					550:559	Surface Electron microscopy	533:559	Surface Electron microscopy (SEM)	533:565	Surface Electron microscopy (SEM) showed a porous architecture varying from 200-400 μm.
34157519	0	79	dep	Fabrication	0:10	arg1	polyphenol					54:63	A plant polyphenol	46:63	Fabrication and characterization of Chrysin - A plant polyphenol loaded alginate -chitosan composite for wound healing application.	0:130	Fabrication and characterization of Chrysin - A plant polyphenol loaded alginate -chitosan composite for wound healing application.
34157519	11	80	theme	fabricated	1452:1461	arg1	scaffolds					1474:1482	fabricated ALG-CS-CHY scaffolds	1452:1482	fabricated ALG-CS-CHY scaffolds	1452:1482	Calcein AM, Propidium iodide, was used for live and dead cell staining, which confirmed that fabricated ALG-CS-CHY scaffolds are biocompatible and facilitate cell growth and cell proliferation.
34157519	12	81	theme	other	1788:1792	arg1	groups					1794:1799	other groups	1788:1799	other groups	1788:1799	In-vivo and in-vitro observations show that the experimental group treated using the ALG-CS-CHY reduces the period of re-epithelization, accelerated fibroblast cell migration, and contracted wound significantly (p < 0.001) compared to other groups.
34601367	0	0	theme	facile	84:89	arg1	preparation					91:101	facile preparation	84:101	facile preparation of novel and green enzyme mimic	84:133	Simultaneously colorimetric detection and effective removal of mercury ion based on facile preparation of novel and green enzyme mimic.
34601367	4	1	theme	portable	739:746	arg1	tube					759:762	a portable analytical tube	737:762	a portable analytical tube containing oxidase mimic	737:787	Benefiting from the thermal stability and the compatibility with carbohydrate, the CS/Cu/Fe composite was further integrated with agarose hydrogel to fabricate a portable analytical tube containing oxidase mimic.
34601367	0	2	dep	enzyme	122:127	arg1	mimic					129:133	mimic	129:133	mimic	129:133	Simultaneously colorimetric detection and effective removal of mercury ion based on facile preparation of novel and green enzyme mimic.
34601367	5	3	from	presence	855:862	arg1	inhibition					803:812	the inhibition	799:812	the inhibition of the catalytic oxidation of TMB in the presence of cysteine	799:874	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	5	3	from	presence	855:862	arg1	recovery					892:899	the recovery	888:899	the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+)	888:1004	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	8	4	theme	Hg2+	1426:1429	arg1	removal					1415:1421	the effective removal	1401:1421	the effective removal of Hg2+	1401:1429	Furthermore, it was found that the effective removal of Hg2+ could be realized in the analytical tube based on efficient Hg2+ adsorption by CS/Cu/Fe composite and agarose hydrogel.
34601367	9	5	attach	remove	1681:1686	arg2	strategy					1642:1649	a smart strategy	1634:1649	a smart strategy to simultaneously monitor and remove toxic Hg2+ from contaminated water	1634:1721	This study not only prepared a robust and low-cost enzyme mimic, but also proposed a smart strategy to simultaneously monitor and remove toxic Hg2+ from contaminated water.
34601367	9	5	attach	remove	1681:1686	arg1	water					1717:1721	contaminated water	1704:1721	contaminated water	1704:1721	This study not only prepared a robust and low-cost enzyme mimic, but also proposed a smart strategy to simultaneously monitor and remove toxic Hg2+ from contaminated water.
34601367	8	6	theme	Hg2+	1491:1494	arg1	adsorption					1496:1505	efficient Hg2+ adsorption	1481:1505	efficient Hg2+ adsorption by CS/Cu/Fe composite and agarose hydrogel	1481:1548	Furthermore, it was found that the effective removal of Hg2+ could be realized in the analytical tube based on efficient Hg2+ adsorption by CS/Cu/Fe composite and agarose hydrogel.
34601367	6	7	theme	colorimetric	1106:1117	arg1	method					1119:1124	This colorimetric method	1101:1124	This colorimetric method	1101:1124	This colorimetric method showed good linear response to Hg2+ over the range from 40 nM to 8.0 μM with a detection limit of 8.9 nM.
34601367	5	8	theme	CS/Cu/Fe	929:936	arg1	activity					917:924	oxidase-like activity	904:924	oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+)	904:1004	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	8	9	theme	efficient	1481:1489	arg1	adsorption					1496:1505	efficient Hg2+ adsorption	1481:1505	efficient Hg2+ adsorption by CS/Cu/Fe composite and agarose hydrogel	1481:1548	Furthermore, it was found that the effective removal of Hg2+ could be realized in the analytical tube based on efficient Hg2+ adsorption by CS/Cu/Fe composite and agarose hydrogel.
34601367	1	10	theme	chitosan/Cu/Fe	256:269	arg1	composite					282:290	chitosan/Cu/Fe (CS/Cu/Fe) composite	256:290	chitosan/Cu/Fe (CS/Cu/Fe) composite	256:290	In this work, an environmentally-friendly and cost-effective enzyme mimic was obtained by facile one-pot preparation of chitosan/Cu/Fe (CS/Cu/Fe) composite.
34601367	9	11	theme	toxic	1688:1692	arg1	Hg2+					1694:1697	toxic Hg2+	1688:1697	toxic Hg2+	1688:1697	This study not only prepared a robust and low-cost enzyme mimic, but also proposed a smart strategy to simultaneously monitor and remove toxic Hg2+ from contaminated water.
34601367	5	12	from	oxidation	831:839	arg1	presence					855:862	the presence	851:862	the presence of cysteine	851:874	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	8	13	theme	effective	1405:1413	arg1	removal					1415:1421	the effective removal	1401:1421	the effective removal of Hg2+	1401:1429	Furthermore, it was found that the effective removal of Hg2+ could be realized in the analytical tube based on efficient Hg2+ adsorption by CS/Cu/Fe composite and agarose hydrogel.
34601367	5	14	theme	mercury	987:993	arg1	ion					995:997	mercury ion	987:997	mercury ion	987:997	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	4	15	dep	oxidase	775:781	arg1	mimic					783:787	mimic	783:787	mimic	783:787	Benefiting from the thermal stability and the compatibility with carbohydrate, the CS/Cu/Fe composite was further integrated with agarose hydrogel to fabricate a portable analytical tube containing oxidase mimic.
34601367	4	16	contain	containing	764:773	arg2	oxidase					775:781	oxidase mimic	775:787	oxidase mimic	775:787	Benefiting from the thermal stability and the compatibility with carbohydrate, the CS/Cu/Fe composite was further integrated with agarose hydrogel to fabricate a portable analytical tube containing oxidase mimic.
34601367	4	16	contain	containing	764:773	arg1	tube					759:762	a portable analytical tube	737:762	a portable analytical tube containing oxidase mimic	737:787	Benefiting from the thermal stability and the compatibility with carbohydrate, the CS/Cu/Fe composite was further integrated with agarose hydrogel to fabricate a portable analytical tube containing oxidase mimic.
34601367	5	17	theme	ion	995:997	arg1	complexation					958:969	the specific complexation	945:969	the specific complexation of cysteine and mercury ion (Hg2+)	945:1004	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	3	18	theme	catalytic	518:526	arg1	mechanism					528:536	the possible catalytic mechanism	505:536	the possible catalytic mechanism	505:536	The CS/Cu/Fe composite was comprehensively characterized and the possible catalytic mechanism was reasonably explored and discussed.
34601367	1	19	theme	CS/Cu/Fe	272:279	arg1	composite					282:290	chitosan/Cu/Fe (CS/Cu/Fe) composite	256:290	chitosan/Cu/Fe (CS/Cu/Fe) composite	256:290	In this work, an environmentally-friendly and cost-effective enzyme mimic was obtained by facile one-pot preparation of chitosan/Cu/Fe (CS/Cu/Fe) composite.
34601367	9	20	theme	smart	1636:1640	arg1	strategy					1642:1649	a smart strategy	1634:1649	a smart strategy to simultaneously monitor and remove toxic Hg2+ from contaminated water	1634:1721	This study not only prepared a robust and low-cost enzyme mimic, but also proposed a smart strategy to simultaneously monitor and remove toxic Hg2+ from contaminated water.
34601367	6	21	from	8.0 μM	1191:1196	arg1	range					1171:1175	the range	1167:1175	the range from 40 nM to 8.0 μM	1167:1196	This colorimetric method showed good linear response to Hg2+ over the range from 40 nM to 8.0 μM with a detection limit of 8.9 nM.
34601367	7	22	theme	tap	1344:1346	arg1	water					1348:1352	tap water	1344:1352	tap water	1344:1352	The method also revealed high selectivity and satisfactory results in recovery experiments of Hg2+ detection in tap water and lake water.
34601367	7	23	theme	detection	1331:1339	arg1	experiments					1311:1321	recovery experiments	1302:1321	recovery experiments of Hg2+ detection in tap water and lake water	1302:1367	The method also revealed high selectivity and satisfactory results in recovery experiments of Hg2+ detection in tap water and lake water.
34601367	0	24	theme	novel	106:110	arg1	enzyme					122:127	novel and green enzyme	106:127	novel and green enzyme mimic	106:133	Simultaneously colorimetric detection and effective removal of mercury ion based on facile preparation of novel and green enzyme mimic.
34601367	8	25	theme	composite	1519:1527	arg1	CS/Cu/Fe					1510:1517	CS/Cu/Fe composite	1510:1527	CS/Cu/Fe composite	1510:1527	Furthermore, it was found that the effective removal of Hg2+ could be realized in the analytical tube based on efficient Hg2+ adsorption by CS/Cu/Fe composite and agarose hydrogel.
34601367	9	26	theme	low-cost	1593:1600	arg1	enzyme					1602:1607	a robust and low-cost enzyme	1580:1607	a robust and low-cost enzyme mimic	1580:1613	This study not only prepared a robust and low-cost enzyme mimic, but also proposed a smart strategy to simultaneously monitor and remove toxic Hg2+ from contaminated water.
34601367	4	27	with	Benefiting	577:586	arg1	carbohydrate					642:653	carbohydrate	642:653	carbohydrate	642:653	Benefiting from the thermal stability and the compatibility with carbohydrate, the CS/Cu/Fe composite was further integrated with agarose hydrogel to fabricate a portable analytical tube containing oxidase mimic.
34601367	4	27	with	Benefiting	577:586	arg1	composite					669:677	the CS/Cu/Fe composite	656:677	the CS/Cu/Fe composite	656:677	Benefiting from the thermal stability and the compatibility with carbohydrate, the CS/Cu/Fe composite was further integrated with agarose hydrogel to fabricate a portable analytical tube containing oxidase mimic.
34601367	1	28	theme	environmentally-friendly	153:176	arg1	enzyme					197:202	an environmentally-friendly and cost-effective enzyme	150:202	an environmentally-friendly and cost-effective enzyme mimic	150:208	In this work, an environmentally-friendly and cost-effective enzyme mimic was obtained by facile one-pot preparation of chitosan/Cu/Fe (CS/Cu/Fe) composite.
34601367	0	29	theme	colorimetric	15:26	arg1	detection					28:36	colorimetric detection	15:36	colorimetric detection	15:36	Simultaneously colorimetric detection and effective removal of mercury ion based on facile preparation of novel and green enzyme mimic.
34601367	2	30	theme	enhanced	332:339	arg1	activity					359:366	significantly enhanced oxidase-mimicking activity	318:366	significantly enhanced oxidase-mimicking activity	318:366	This composite exhibited significantly enhanced oxidase-mimicking activity during catalyzing the oxidation of 3, 3', 5, 5'-tetramethylbenzidine (TMB).
34601367	8	31	theme	agarose	1533:1539	arg1	hydrogel					1541:1548	agarose hydrogel	1533:1548	agarose hydrogel	1533:1548	Furthermore, it was found that the effective removal of Hg2+ could be realized in the analytical tube based on efficient Hg2+ adsorption by CS/Cu/Fe composite and agarose hydrogel.
34601367	8	32	theme	analytical	1456:1465	arg1	tube					1467:1470	the analytical tube	1452:1470	the analytical tube based on efficient Hg2+ adsorption by CS/Cu/Fe composite and agarose hydrogel	1452:1548	Furthermore, it was found that the effective removal of Hg2+ could be realized in the analytical tube based on efficient Hg2+ adsorption by CS/Cu/Fe composite and agarose hydrogel.
34601367	7	33	from	water	1363:1367	arg1	experiments					1311:1321	recovery experiments	1302:1321	recovery experiments of Hg2+ detection in tap water and lake water	1302:1367	The method also revealed high selectivity and satisfactory results in recovery experiments of Hg2+ detection in tap water and lake water.
34601367	1	34	theme	cost-effective	182:195	arg1	enzyme					197:202	an environmentally-friendly and cost-effective enzyme	150:202	an environmentally-friendly and cost-effective enzyme mimic	150:208	In this work, an environmentally-friendly and cost-effective enzyme mimic was obtained by facile one-pot preparation of chitosan/Cu/Fe (CS/Cu/Fe) composite.
34601367	1	35	theme	composite	282:290	arg1	preparation					241:251	facile one-pot preparation	226:251	facile one-pot preparation of chitosan/Cu/Fe (CS/Cu/Fe) composite	226:290	In this work, an environmentally-friendly and cost-effective enzyme mimic was obtained by facile one-pot preparation of chitosan/Cu/Fe (CS/Cu/Fe) composite.
34601367	0	36	theme	enzyme	122:127	arg1	preparation					91:101	facile preparation	84:101	facile preparation of novel and green enzyme mimic	84:133	Simultaneously colorimetric detection and effective removal of mercury ion based on facile preparation of novel and green enzyme mimic.
34601367	5	37	theme	specific	949:956	arg1	complexation					958:969	the specific complexation	945:969	the specific complexation of cysteine and mercury ion (Hg2+)	945:1004	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	5	38	theme	cysteine	974:981	arg1	complexation					958:969	the specific complexation	945:969	the specific complexation of cysteine and mercury ion (Hg2+)	945:1004	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	5	39	theme	cysteine	867:874	arg1	presence					855:862	the presence	851:862	the presence of cysteine	851:874	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	4	40	theme	thermal	597:603	arg1	stability					605:613	the thermal stability	593:613	the thermal stability	593:613	Benefiting from the thermal stability and the compatibility with carbohydrate, the CS/Cu/Fe composite was further integrated with agarose hydrogel to fabricate a portable analytical tube containing oxidase mimic.
34601367	0	41	theme	green	116:120	arg1	enzyme					122:127	novel and green enzyme	106:127	novel and green enzyme mimic	106:133	Simultaneously colorimetric detection and effective removal of mercury ion based on facile preparation of novel and green enzyme mimic.
34601367	9	42	dep	enzyme	1602:1607	arg1	mimic					1609:1613	mimic	1609:1613	mimic	1609:1613	This study not only prepared a robust and low-cost enzyme mimic, but also proposed a smart strategy to simultaneously monitor and remove toxic Hg2+ from contaminated water.
34601367	4	43	theme	CS/Cu/Fe	660:667	arg1	carbohydrate					642:653	carbohydrate	642:653	carbohydrate	642:653	Benefiting from the thermal stability and the compatibility with carbohydrate, the CS/Cu/Fe composite was further integrated with agarose hydrogel to fabricate a portable analytical tube containing oxidase mimic.
34601367	4	43	theme	CS/Cu/Fe	660:667	arg1	composite					669:677	the CS/Cu/Fe composite	656:677	the CS/Cu/Fe composite	656:677	Benefiting from the thermal stability and the compatibility with carbohydrate, the CS/Cu/Fe composite was further integrated with agarose hydrogel to fabricate a portable analytical tube containing oxidase mimic.
34601367	5	44	theme	catalytic	821:829	arg1	oxidation					831:839	the catalytic oxidation	817:839	the catalytic oxidation of TMB in the presence of cysteine	817:874	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	4	45	theme	analytical	748:757	arg1	tube					759:762	a portable analytical tube	737:762	a portable analytical tube containing oxidase mimic	737:787	Benefiting from the thermal stability and the compatibility with carbohydrate, the CS/Cu/Fe composite was further integrated with agarose hydrogel to fabricate a portable analytical tube containing oxidase mimic.
34601367	7	46	from	water	1348:1352	arg1	experiments					1311:1321	recovery experiments	1302:1321	recovery experiments of Hg2+ detection in tap water and lake water	1302:1367	The method also revealed high selectivity and satisfactory results in recovery experiments of Hg2+ detection in tap water and lake water.
34601367	6	47	theme	linear	1138:1143	arg1	response					1145:1152	good linear response	1133:1152	good linear response to Hg2+	1133:1160	This colorimetric method showed good linear response to Hg2+ over the range from 40 nM to 8.0 μM with a detection limit of 8.9 nM.
34601367	5	48	theme	colorimetric	1017:1028	arg1	detection					1030:1038	the rapid colorimetric detection	1007:1038	the rapid colorimetric detection of Hg2+	1007:1046	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	4	49	theme	agarose	707:713	arg1	hydrogel					715:722	agarose hydrogel	707:722	agarose hydrogel	707:722	Benefiting from the thermal stability and the compatibility with carbohydrate, the CS/Cu/Fe composite was further integrated with agarose hydrogel to fabricate a portable analytical tube containing oxidase mimic.
34601367	3	50	theme	CS/Cu/Fe	448:455	arg1	composite					457:465	The CS/Cu/Fe composite	444:465	The CS/Cu/Fe composite	444:465	The CS/Cu/Fe composite was comprehensively characterized and the possible catalytic mechanism was reasonably explored and discussed.
34601367	0	51	theme	effective	42:50	arg1	removal					52:58	effective removal	42:58	effective removal of mercury ion	42:73	Simultaneously colorimetric detection and effective removal of mercury ion based on facile preparation of novel and green enzyme mimic.
34601367	5	52	theme	oxidation	831:839	arg1	inhibition					803:812	the inhibition	799:812	the inhibition of the catalytic oxidation of TMB in the presence of cysteine	799:874	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	5	52	theme	oxidation	831:839	arg1	recovery					892:899	the recovery	888:899	the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+)	888:1004	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	5	53	theme	analytical	1084:1093	arg1	tube					1095:1098	the analytical tube	1080:1098	the analytical tube	1080:1098	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	6	54	theme	8.9 nM	1224:1229	arg1	limit					1215:1219	a detection limit	1203:1219	a detection limit of 8.9 nM	1203:1229	This colorimetric method showed good linear response to Hg2+ over the range from 40 nM to 8.0 μM with a detection limit of 8.9 nM.
34601367	4	55	from	stability	605:613	arg1	compatibility					623:635	the compatibility	619:635	the compatibility with carbohydrate, the CS/Cu/Fe composite	619:677	Benefiting from the thermal stability and the compatibility with carbohydrate, the CS/Cu/Fe composite was further integrated with agarose hydrogel to fabricate a portable analytical tube containing oxidase mimic.
34601367	4	55	from	stability	605:613	arg1	Benefiting					577:586	Benefiting	577:586	Benefiting from the thermal stability	577:613	Benefiting from the thermal stability and the compatibility with carbohydrate, the CS/Cu/Fe composite was further integrated with agarose hydrogel to fabricate a portable analytical tube containing oxidase mimic.
34601367	0	56	theme	mercury	63:69	arg1	ion					71:73	mercury ion	63:73	mercury ion	63:73	Simultaneously colorimetric detection and effective removal of mercury ion based on facile preparation of novel and green enzyme mimic.
34601367	5	57	theme	oxidase-like	904:915	arg1	activity					917:924	oxidase-like activity	904:924	oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+)	904:1004	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	9	58	theme	robust	1582:1587	arg1	enzyme					1602:1607	a robust and low-cost enzyme	1580:1607	a robust and low-cost enzyme mimic	1580:1613	This study not only prepared a robust and low-cost enzyme mimic, but also proposed a smart strategy to simultaneously monitor and remove toxic Hg2+ from contaminated water.
34601367	5	59	theme	due	938:940	arg1	CS/Cu/Fe					929:936	CS/Cu/Fe	929:936	CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+)	929:1004	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	5	60	theme	TMB	844:846	arg1	oxidation					831:839	the catalytic oxidation	817:839	the catalytic oxidation of TMB in the presence of cysteine	817:874	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	7	61	theme	Hg2+	1326:1329	arg1	detection					1331:1339	Hg2+ detection	1326:1339	Hg2+ detection in tap water and lake water	1326:1367	The method also revealed high selectivity and satisfactory results in recovery experiments of Hg2+ detection in tap water and lake water.
34601367	5	62	theme	Hg2+	1043:1046	arg1	detection					1030:1038	the rapid colorimetric detection	1007:1038	the rapid colorimetric detection of Hg2+	1007:1046	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	3	63	theme	possible	509:516	arg1	mechanism					528:536	the possible catalytic mechanism	505:536	the possible catalytic mechanism	505:536	The CS/Cu/Fe composite was comprehensively characterized and the possible catalytic mechanism was reasonably explored and discussed.
34601367	9	64	theme	contaminated	1704:1715	arg1	water					1717:1721	contaminated water	1704:1721	contaminated water	1704:1721	This study not only prepared a robust and low-cost enzyme mimic, but also proposed a smart strategy to simultaneously monitor and remove toxic Hg2+ from contaminated water.
34601367	6	65	theme	detection	1205:1213	arg1	limit					1215:1219	a detection limit	1203:1219	a detection limit of 8.9 nM	1203:1229	This colorimetric method showed good linear response to Hg2+ over the range from 40 nM to 8.0 μM with a detection limit of 8.9 nM.
34601367	4	66	with	compatibility	623:635	arg1	carbohydrate					642:653	carbohydrate	642:653	carbohydrate	642:653	Benefiting from the thermal stability and the compatibility with carbohydrate, the CS/Cu/Fe composite was further integrated with agarose hydrogel to fabricate a portable analytical tube containing oxidase mimic.
34601367	4	66	with	compatibility	623:635	arg1	composite					669:677	the CS/Cu/Fe composite	656:677	the CS/Cu/Fe composite	656:677	Benefiting from the thermal stability and the compatibility with carbohydrate, the CS/Cu/Fe composite was further integrated with agarose hydrogel to fabricate a portable analytical tube containing oxidase mimic.
34601367	7	67	from	experiments	1311:1321	arg1	water					1348:1352	tap water	1344:1352	tap water	1344:1352	The method also revealed high selectivity and satisfactory results in recovery experiments of Hg2+ detection in tap water and lake water.
34601367	7	67	from	experiments	1311:1321	arg1	water					1363:1367	lake water	1358:1367	lake water	1358:1367	The method also revealed high selectivity and satisfactory results in recovery experiments of Hg2+ detection in tap water and lake water.
34601367	6	68	theme	good	1133:1136	arg1	response					1145:1152	good linear response	1133:1152	good linear response to Hg2+	1133:1160	This colorimetric method showed good linear response to Hg2+ over the range from 40 nM to 8.0 μM with a detection limit of 8.9 nM.
34601367	7	69	from	detection	1331:1339	arg1	water					1348:1352	tap water	1344:1352	tap water	1344:1352	The method also revealed high selectivity and satisfactory results in recovery experiments of Hg2+ detection in tap water and lake water.
34601367	7	69	from	detection	1331:1339	arg1	water					1363:1367	lake water	1358:1367	lake water	1358:1367	The method also revealed high selectivity and satisfactory results in recovery experiments of Hg2+ detection in tap water and lake water.
34601367	6	70	dep	8.0 μM	1191:1196	arg1	to					1188:1189	to	1188:1189	to	1188:1189	This colorimetric method showed good linear response to Hg2+ over the range from 40 nM to 8.0 μM with a detection limit of 8.9 nM.
34601367	7	71	theme	satisfactory	1278:1289	arg1	results					1291:1297	satisfactory results	1278:1297	satisfactory results	1278:1297	The method also revealed high selectivity and satisfactory results in recovery experiments of Hg2+ detection in tap water and lake water.
34601367	7	72	theme	lake	1358:1361	arg1	water					1363:1367	lake water	1358:1367	lake water	1358:1367	The method also revealed high selectivity and satisfactory results in recovery experiments of Hg2+ detection in tap water and lake water.
34601367	5	73	from	recovery	892:899	arg1	presence					855:862	the presence	851:862	the presence of cysteine	851:874	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	2	74	theme	oxidase-mimicking	341:357	arg1	activity					359:366	significantly enhanced oxidase-mimicking activity	318:366	significantly enhanced oxidase-mimicking activity	318:366	This composite exhibited significantly enhanced oxidase-mimicking activity during catalyzing the oxidation of 3, 3', 5, 5'-tetramethylbenzidine (TMB).
34601367	1	75	theme	facile	226:231	arg1	preparation					241:251	facile one-pot preparation	226:251	facile one-pot preparation of chitosan/Cu/Fe (CS/Cu/Fe) composite	226:290	In this work, an environmentally-friendly and cost-effective enzyme mimic was obtained by facile one-pot preparation of chitosan/Cu/Fe (CS/Cu/Fe) composite.
34601367	5	76	from	inhibition	803:812	arg1	presence					855:862	the presence	851:862	the presence of cysteine	851:874	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	1	77	dep	enzyme	197:202	arg1	mimic					204:208	mimic	204:208	mimic	204:208	In this work, an environmentally-friendly and cost-effective enzyme mimic was obtained by facile one-pot preparation of chitosan/Cu/Fe (CS/Cu/Fe) composite.
34601367	7	78	theme	recovery	1302:1309	arg1	experiments					1311:1321	recovery experiments	1302:1321	recovery experiments of Hg2+ detection in tap water and lake water	1302:1367	The method also revealed high selectivity and satisfactory results in recovery experiments of Hg2+ detection in tap water and lake water.
34601367	2	79	theme	3	403:403	arg1	oxidation					390:398	the oxidation	386:398	the oxidation of 3, 3', 5, 5'-tetramethylbenzidine (TMB)	386:441	This composite exhibited significantly enhanced oxidase-mimicking activity during catalyzing the oxidation of 3, 3', 5, 5'-tetramethylbenzidine (TMB).
34601367	1	80	theme	one-pot	233:239	arg1	preparation					241:251	facile one-pot preparation	226:251	facile one-pot preparation of chitosan/Cu/Fe (CS/Cu/Fe) composite	226:290	In this work, an environmentally-friendly and cost-effective enzyme mimic was obtained by facile one-pot preparation of chitosan/Cu/Fe (CS/Cu/Fe) composite.
34601367	0	81	theme	ion	71:73	arg1	detection					28:36	colorimetric detection	15:36	colorimetric detection	15:36	Simultaneously colorimetric detection and effective removal of mercury ion based on facile preparation of novel and green enzyme mimic.
34601367	0	81	theme	ion	71:73	arg1	removal					52:58	effective removal	42:58	effective removal of mercury ion	42:73	Simultaneously colorimetric detection and effective removal of mercury ion based on facile preparation of novel and green enzyme mimic.
34601367	5	82	theme	rapid	1011:1015	arg1	detection					1030:1038	the rapid colorimetric detection	1007:1038	the rapid colorimetric detection of Hg2+	1007:1046	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	7	83	theme	high	1257:1260	arg1	selectivity					1262:1272	high selectivity	1257:1272	high selectivity	1257:1272	The method also revealed high selectivity and satisfactory results in recovery experiments of Hg2+ detection in tap water and lake water.
34601367	5	84	theme	activity	917:924	arg1	inhibition					803:812	the inhibition	799:812	the inhibition of the catalytic oxidation of TMB in the presence of cysteine	799:874	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
34601367	5	84	theme	activity	917:924	arg1	recovery					892:899	the recovery	888:899	the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+)	888:1004	Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg2+), the rapid colorimetric detection of Hg2+ was successfully carried out in the analytical tube.
33190295	5	0	theme	thaliana	836:843	arg1	walls					815:819	the cell walls	806:819	the cell walls of Arabidopsis thaliana	806:843	In the present study, we used a combination of immunohistochemical, chemical and spectroscopic analyses to characterize the cell walls of Arabidopsis thaliana and characterized a mutant in the XTH19 gene, encoding a xyloglucan endotransglucosylase/hydrolase (XTH).
33190295	7	1	theme	LM25	1307:1310	arg1	epitopes					1262:1269	epitopes	1262:1269	epitopes of the xyloglucan-specific antibody LM25	1262:1310	Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant.
33190295	2	2	dep	low	335:337	arg1	non-freezing					340:351	non-freezing	340:351	non-freezing	340:351	Many plant species increase their freezing tolerance during exposure to low, non-freezing temperatures, a process termed cold acclimation.
33190295	8	3	theme	temperature	1571:1581	arg1	acclimation					1583:1593	low temperature acclimation	1567:1593	low temperature acclimation	1567:1593	Taken together, our data shed new light on the potential roles of cell wall remodelling for the increased freezing tolerance observed after low temperature acclimation.
33190295	7	4	theme	3D	1207:1208	arg1	reconstruction					1210:1223	3D reconstruction	1207:1223	3D reconstruction of centres of rosette	1207:1245	Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant.
33190295	7	5	theme	xyloglucan-specific	1278:1296	arg1	LM25					1307:1310	the xyloglucan-specific antibody LM25	1274:1310	the xyloglucan-specific antibody LM25	1274:1310	Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant.
33190295	4	6	theme	transcriptome	558:570	arg1	analyses					585:592	Previous transcriptome and proteome analyses	549:592	Previous transcriptome and proteome analyses	549:592	Previous transcriptome and proteome analyses indicate that cell wall remodelling may be particularly important for sub-zero acclimation.
33190295	1	7	dep	cell	202:205	arg1	deformation					223:233	deformation	223:233	deformation	223:233	Freezing triggers extracellular ice formation leading to cell dehydration and deformation during a freeze-thaw cycle.
33190295	1	7	dep	cell	202:205	arg1	dehydration					207:217	dehydration	207:217	dehydration	207:217	Freezing triggers extracellular ice formation leading to cell dehydration and deformation during a freeze-thaw cycle.
33190295	2	8	theme	low	335:337	arg1	temperatures					353:364	low, non-freezing temperatures	335:364	low, non-freezing temperatures	335:364	Many plant species increase their freezing tolerance during exposure to low, non-freezing temperatures, a process termed cold acclimation.
33190295	2	8	theme	low	335:337	arg1	process					369:375	a process	367:375	a process termed cold acclimation	367:399	Many plant species increase their freezing tolerance during exposure to low, non-freezing temperatures, a process termed cold acclimation.
33190295	6	9	theme	wild	1060:1063	arg1	type					1065:1068	the Col-0 wild type	1050:1068	the Col-0 wild type	1050:1068	The mutant showed reduced freezing tolerance after both cold and sub-zero acclimation, compared to the Col-0 wild type, which was associated with differences in cell wall composition and structure.
33190295	7	10	theme	antibody	1298:1305	arg1	LM25					1307:1310	the xyloglucan-specific antibody LM25	1274:1310	the xyloglucan-specific antibody LM25	1274:1310	Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant.
33190295	6	11	theme	reduced	969:975	arg1	tolerance					986:994	reduced freezing tolerance	969:994	reduced freezing tolerance	969:994	The mutant showed reduced freezing tolerance after both cold and sub-zero acclimation, compared to the Col-0 wild type, which was associated with differences in cell wall composition and structure.
33190295	6	12	theme	Col-0	1054:1058	arg1	type					1065:1068	the Col-0 wild type	1050:1068	the Col-0 wild type	1050:1068	The mutant showed reduced freezing tolerance after both cold and sub-zero acclimation, compared to the Col-0 wild type, which was associated with differences in cell wall composition and structure.
33190295	7	13	theme	centres	1228:1234	arg1	reconstruction					1210:1223	3D reconstruction	1207:1223	3D reconstruction of centres of rosette	1207:1245	Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant.
33190295	3	14	theme	sub-zero	526:533	arg1	acclimation					535:545	sub-zero acclimation	526:545	sub-zero acclimation	526:545	In addition, exposure to mild freezing temperatures after cold acclimation evokes a further increase in freezing tolerance (sub-zero acclimation).
33190295	3	14	theme	sub-zero	526:533	arg1	tolerance					515:523	freezing tolerance	506:523	freezing tolerance (sub-zero acclimation)	506:546	In addition, exposure to mild freezing temperatures after cold acclimation evokes a further increase in freezing tolerance (sub-zero acclimation).
33190295	5	15	used	used	711:714	arg2	we					708:709	we	708:709	we	708:709	In the present study, we used a combination of immunohistochemical, chemical and spectroscopic analyses to characterize the cell walls of Arabidopsis thaliana and characterized a mutant in the XTH19 gene, encoding a xyloglucan endotransglucosylase/hydrolase (XTH).
33190295	6	16	dep	wall	1117:1120	arg1	structure					1138:1146	structure	1138:1146	structure	1138:1146	The mutant showed reduced freezing tolerance after both cold and sub-zero acclimation, compared to the Col-0 wild type, which was associated with differences in cell wall composition and structure.
33190295	6	16	dep	wall	1117:1120	arg1	composition					1122:1132	composition	1122:1132	composition	1122:1132	The mutant showed reduced freezing tolerance after both cold and sub-zero acclimation, compared to the Col-0 wild type, which was associated with differences in cell wall composition and structure.
33190295	0	17	theme	sub-zero	123:130	arg1	acclimation					132:142	sub-zero acclimation	123:142	sub-zero acclimation	123:142	Cell wall modification by the xyloglucan endotransglucosylase/hydrolase XTH19 influences freezing tolerance after cold and sub-zero acclimation.
33190295	5	18	theme	present	693:699	arg1	study					701:705	the present study	689:705	the present study	689:705	In the present study, we used a combination of immunohistochemical, chemical and spectroscopic analyses to characterize the cell walls of Arabidopsis thaliana and characterized a mutant in the XTH19 gene, encoding a xyloglucan endotransglucosylase/hydrolase (XTH).
33190295	0	19	theme	Cell	0:3	arg1	wall					5:8	Cell wall	0:8	Cell wall modification by the xyloglucan endotransglucosylase/hydrolase XTH19	0:76	Cell wall modification by the xyloglucan endotransglucosylase/hydrolase XTH19 influences freezing tolerance after cold and sub-zero acclimation.
33190295	3	20	theme	further	486:492	arg1	increase					494:501	a further increase	484:501	a further increase in freezing tolerance (sub-zero acclimation)	484:546	In addition, exposure to mild freezing temperatures after cold acclimation evokes a further increase in freezing tolerance (sub-zero acclimation).
33190295	0	21	theme	wall	5:8	arg1	modification					10:21	Cell wall modification	0:21	Cell wall modification by the xyloglucan endotransglucosylase/hydrolase XTH19	0:76	Cell wall modification by the xyloglucan endotransglucosylase/hydrolase XTH19 influences freezing tolerance after cold and sub-zero acclimation.
33190295	6	22	from	differences	1097:1107	arg1	wall					1117:1120	cell wall composition and structure	1112:1146	cell wall composition and structure	1112:1146	The mutant showed reduced freezing tolerance after both cold and sub-zero acclimation, compared to the Col-0 wild type, which was associated with differences in cell wall composition and structure.
33190295	7	23	theme	plants	1361:1366	arg1	vasculature					1340:1350	the vasculature	1336:1350	the vasculature of Col-0 plants after sub-zero acclimation	1336:1393	Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant.
33190295	3	24	theme	mild	427:430	arg1	temperatures					441:452	mild freezing temperatures	427:452	mild freezing temperatures	427:452	In addition, exposure to mild freezing temperatures after cold acclimation evokes a further increase in freezing tolerance (sub-zero acclimation).
33190295	4	25	theme	wall	613:616	arg1	remodelling					618:628	cell wall remodelling	608:628	cell wall remodelling	608:628	Previous transcriptome and proteome analyses indicate that cell wall remodelling may be particularly important for sub-zero acclimation.
33190295	5	26	theme	XTH19	879:883	arg1	gene					885:888	the XTH19 gene	875:888	the XTH19 gene	875:888	In the present study, we used a combination of immunohistochemical, chemical and spectroscopic analyses to characterize the cell walls of Arabidopsis thaliana and characterized a mutant in the XTH19 gene, encoding a xyloglucan endotransglucosylase/hydrolase (XTH).
33190295	7	27	theme	XTH19	1413:1417	arg1	mutant					1419:1424	the XTH19 mutant	1409:1424	the XTH19 mutant	1409:1424	Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant.
33190295	7	28	theme	rosette	1239:1245	arg1	centres					1228:1234	centres	1228:1234	centres of rosette	1228:1245	Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant.
33190295	4	29	theme	cell	608:611	arg1	remodelling					618:628	cell wall remodelling	608:628	cell wall remodelling	608:628	Previous transcriptome and proteome analyses indicate that cell wall remodelling may be particularly important for sub-zero acclimation.
33190295	3	30	theme	freezing	506:513	arg1	acclimation					535:545	sub-zero acclimation	526:545	sub-zero acclimation	526:545	In addition, exposure to mild freezing temperatures after cold acclimation evokes a further increase in freezing tolerance (sub-zero acclimation).
33190295	3	30	theme	freezing	506:513	arg1	tolerance					515:523	freezing tolerance	506:523	freezing tolerance (sub-zero acclimation)	506:546	In addition, exposure to mild freezing temperatures after cold acclimation evokes a further increase in freezing tolerance (sub-zero acclimation).
33190295	5	31	theme	spectroscopic	767:779	arg1	analyses					781:788	immunohistochemical, chemical and spectroscopic analyses	733:788	immunohistochemical, chemical and spectroscopic analyses	733:788	In the present study, we used a combination of immunohistochemical, chemical and spectroscopic analyses to characterize the cell walls of Arabidopsis thaliana and characterized a mutant in the XTH19 gene, encoding a xyloglucan endotransglucosylase/hydrolase (XTH).
33190295	8	32	theme	new	1457:1459	arg1	light					1461:1465	new light	1457:1465	new light	1457:1465	Taken together, our data shed new light on the potential roles of cell wall remodelling for the increased freezing tolerance observed after low temperature acclimation.
33190295	6	33	theme	cell	1112:1115	arg1	wall					1117:1120	cell wall composition and structure	1112:1146	cell wall composition and structure	1112:1146	The mutant showed reduced freezing tolerance after both cold and sub-zero acclimation, compared to the Col-0 wild type, which was associated with differences in cell wall composition and structure.
33190295	0	34	theme	xyloglucan	30:39	arg1	XTH19					72:76	the xyloglucan endotransglucosylase/hydrolase XTH19	26:76	the xyloglucan endotransglucosylase/hydrolase XTH19	26:76	Cell wall modification by the xyloglucan endotransglucosylase/hydrolase XTH19 influences freezing tolerance after cold and sub-zero acclimation.
33190295	7	35	from	immunohistochemistry	1166:1185	arg1	combination					1190:1200	combination	1190:1200	combination with 3D reconstruction of centres of rosette	1190:1245	Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant.
33190295	5	36	theme	analyses	781:788	arg1	combination					718:728	a combination	716:728	a combination of immunohistochemical, chemical and spectroscopic analyses	716:788	In the present study, we used a combination of immunohistochemical, chemical and spectroscopic analyses to characterize the cell walls of Arabidopsis thaliana and characterized a mutant in the XTH19 gene, encoding a xyloglucan endotransglucosylase/hydrolase (XTH).
33190295	5	37	theme	xyloglucan	902:911	arg1	XTH					945:947	XTH	945:947	XTH	945:947	In the present study, we used a combination of immunohistochemical, chemical and spectroscopic analyses to characterize the cell walls of Arabidopsis thaliana and characterized a mutant in the XTH19 gene, encoding a xyloglucan endotransglucosylase/hydrolase (XTH).
33190295	5	37	theme	xyloglucan	902:911	arg1	endotransglucosylase/hydrolase					913:942	a xyloglucan endotransglucosylase/hydrolase	900:942	a xyloglucan endotransglucosylase/hydrolase (XTH)	900:948	In the present study, we used a combination of immunohistochemical, chemical and spectroscopic analyses to characterize the cell walls of Arabidopsis thaliana and characterized a mutant in the XTH19 gene, encoding a xyloglucan endotransglucosylase/hydrolase (XTH).
33190295	1	38	theme	freeze-thaw	244:254	arg1	cycle					256:260	a freeze-thaw cycle	242:260	a freeze-thaw cycle	242:260	Freezing triggers extracellular ice formation leading to cell dehydration and deformation during a freeze-thaw cycle.
33190295	3	39	theme	cold	460:463	arg1	acclimation					465:475	cold acclimation	460:475	cold acclimation	460:475	In addition, exposure to mild freezing temperatures after cold acclimation evokes a further increase in freezing tolerance (sub-zero acclimation).
33190295	8	40	theme	freezing	1533:1540	arg1	tolerance					1542:1550	the increased freezing tolerance	1519:1550	the increased freezing tolerance observed after low temperature acclimation	1519:1593	Taken together, our data shed new light on the potential roles of cell wall remodelling for the increased freezing tolerance observed after low temperature acclimation.
33190295	2	41	theme	plant	268:272	arg1	species					274:280	Many plant species	263:280	Many plant species	263:280	Many plant species increase their freezing tolerance during exposure to low, non-freezing temperatures, a process termed cold acclimation.
33190295	3	42	from	increase	494:501	arg1	acclimation					535:545	sub-zero acclimation	526:545	sub-zero acclimation	526:545	In addition, exposure to mild freezing temperatures after cold acclimation evokes a further increase in freezing tolerance (sub-zero acclimation).
33190295	3	42	from	increase	494:501	arg1	tolerance					515:523	freezing tolerance	506:523	freezing tolerance (sub-zero acclimation)	506:546	In addition, exposure to mild freezing temperatures after cold acclimation evokes a further increase in freezing tolerance (sub-zero acclimation).
33190295	3	43	theme	freezing	432:439	arg1	temperatures					441:452	mild freezing temperatures	427:452	mild freezing temperatures	427:452	In addition, exposure to mild freezing temperatures after cold acclimation evokes a further increase in freezing tolerance (sub-zero acclimation).
33190295	4	44	theme	proteome	576:583	arg1	analyses					585:592	Previous transcriptome and proteome analyses	549:592	Previous transcriptome and proteome analyses	549:592	Previous transcriptome and proteome analyses indicate that cell wall remodelling may be particularly important for sub-zero acclimation.
33190295	8	45	theme	increased	1523:1531	arg1	tolerance					1542:1550	the increased freezing tolerance	1519:1550	the increased freezing tolerance observed after low temperature acclimation	1519:1593	Taken together, our data shed new light on the potential roles of cell wall remodelling for the increased freezing tolerance observed after low temperature acclimation.
33190295	2	46	theme	Many	263:266	arg1	species					274:280	Many plant species	263:280	Many plant species	263:280	Many plant species increase their freezing tolerance during exposure to low, non-freezing temperatures, a process termed cold acclimation.
33190295	2	47	theme	cold	384:387	arg1	acclimation					389:399	cold acclimation	384:399	cold acclimation	384:399	Many plant species increase their freezing tolerance during exposure to low, non-freezing temperatures, a process termed cold acclimation.
33190295	0	48	theme	endotransglucosylase/hydrolase	41:70	arg1	XTH19					72:76	the xyloglucan endotransglucosylase/hydrolase XTH19	26:76	the xyloglucan endotransglucosylase/hydrolase XTH19	26:76	Cell wall modification by the xyloglucan endotransglucosylase/hydrolase XTH19 influences freezing tolerance after cold and sub-zero acclimation.
33190295	5	49	theme	immunohistochemical	733:751	arg1	analyses					781:788	immunohistochemical, chemical and spectroscopic analyses	733:788	immunohistochemical, chemical and spectroscopic analyses	733:788	In the present study, we used a combination of immunohistochemical, chemical and spectroscopic analyses to characterize the cell walls of Arabidopsis thaliana and characterized a mutant in the XTH19 gene, encoding a xyloglucan endotransglucosylase/hydrolase (XTH).
33190295	7	50	from	vasculature	1340:1350	arg1	abundant					1324:1331	abundant	1324:1331	abundant	1324:1331	Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant.
33190295	8	51	theme	potential	1474:1482	arg1	roles					1484:1488	the potential roles	1470:1488	the potential roles of cell wall remodelling for the increased freezing tolerance observed after low temperature acclimation	1470:1593	Taken together, our data shed new light on the potential roles of cell wall remodelling for the increased freezing tolerance observed after low temperature acclimation.
33190295	7	52	from	absent	1399:1404	arg1	mutant					1419:1424	the XTH19 mutant	1409:1424	the XTH19 mutant	1409:1424	Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant.
33190295	7	53	theme	sub-zero	1374:1381	arg1	acclimation					1383:1393	sub-zero acclimation	1374:1393	sub-zero acclimation	1374:1393	Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant.
33190295	8	54	theme	cell	1493:1496	arg1	remodelling					1503:1513	cell wall remodelling	1493:1513	cell wall remodelling	1493:1513	Taken together, our data shed new light on the potential roles of cell wall remodelling for the increased freezing tolerance observed after low temperature acclimation.
33190295	7	55	theme	Col-0	1355:1359	arg1	plants					1361:1366	Col-0 plants	1355:1366	Col-0 plants	1355:1366	Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant.
33190295	5	56	theme	cell	810:813	arg1	walls					815:819	the cell walls	806:819	the cell walls of Arabidopsis thaliana	806:843	In the present study, we used a combination of immunohistochemical, chemical and spectroscopic analyses to characterize the cell walls of Arabidopsis thaliana and characterized a mutant in the XTH19 gene, encoding a xyloglucan endotransglucosylase/hydrolase (XTH).
33190295	5	57	theme	chemical	754:761	arg1	analyses					781:788	immunohistochemical, chemical and spectroscopic analyses	733:788	immunohistochemical, chemical and spectroscopic analyses	733:788	In the present study, we used a combination of immunohistochemical, chemical and spectroscopic analyses to characterize the cell walls of Arabidopsis thaliana and characterized a mutant in the XTH19 gene, encoding a xyloglucan endotransglucosylase/hydrolase (XTH).
33190295	8	58	theme	low	1567:1569	arg1	acclimation					1583:1593	low temperature acclimation	1567:1593	low temperature acclimation	1567:1593	Taken together, our data shed new light on the potential roles of cell wall remodelling for the increased freezing tolerance observed after low temperature acclimation.
33190295	2	59	theme	freezing	297:304	arg1	tolerance					306:314	their freezing tolerance	291:314	their freezing tolerance	291:314	Many plant species increase their freezing tolerance during exposure to low, non-freezing temperatures, a process termed cold acclimation.
33190295	1	60	theme	extracellular	163:175	arg1	formation					181:189	extracellular ice formation	163:189	extracellular ice formation leading to cell dehydration and deformation	163:233	Freezing triggers extracellular ice formation leading to cell dehydration and deformation during a freeze-thaw cycle.
33190295	4	61	theme	sub-zero	664:671	arg1	acclimation					673:683	sub-zero acclimation	664:683	sub-zero acclimation	664:683	Previous transcriptome and proteome analyses indicate that cell wall remodelling may be particularly important for sub-zero acclimation.
33190295	0	62	theme	freezing	89:96	arg1	tolerance					98:106	freezing tolerance	89:106	freezing tolerance	89:106	Cell wall modification by the xyloglucan endotransglucosylase/hydrolase XTH19 influences freezing tolerance after cold and sub-zero acclimation.
33190295	7	63	from	abundant	1324:1331	arg1	vasculature					1340:1350	the vasculature	1336:1350	the vasculature of Col-0 plants after sub-zero acclimation	1336:1393	Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant.
33190295	8	64	theme	remodelling	1503:1513	arg1	roles					1484:1488	the potential roles	1470:1488	the potential roles of cell wall remodelling for the increased freezing tolerance observed after low temperature acclimation	1470:1593	Taken together, our data shed new light on the potential roles of cell wall remodelling for the increased freezing tolerance observed after low temperature acclimation.
33190295	1	65	theme	ice	177:179	arg1	formation					181:189	extracellular ice formation	163:189	extracellular ice formation leading to cell dehydration and deformation	163:233	Freezing triggers extracellular ice formation leading to cell dehydration and deformation during a freeze-thaw cycle.
33190295	6	66	theme	freezing	977:984	arg1	tolerance					986:994	reduced freezing tolerance	969:994	reduced freezing tolerance	969:994	The mutant showed reduced freezing tolerance after both cold and sub-zero acclimation, compared to the Col-0 wild type, which was associated with differences in cell wall composition and structure.
33190295	5	67	theme	Arabidopsis	824:834	arg1	thaliana					836:843	Arabidopsis thaliana	824:843	Arabidopsis thaliana	824:843	In the present study, we used a combination of immunohistochemical, chemical and spectroscopic analyses to characterize the cell walls of Arabidopsis thaliana and characterized a mutant in the XTH19 gene, encoding a xyloglucan endotransglucosylase/hydrolase (XTH).
33190295	4	68	theme	Previous	549:556	arg1	analyses					585:592	Previous transcriptome and proteome analyses	549:592	Previous transcriptome and proteome analyses	549:592	Previous transcriptome and proteome analyses indicate that cell wall remodelling may be particularly important for sub-zero acclimation.
33190295	8	69	theme	wall	1498:1501	arg1	remodelling					1503:1513	cell wall remodelling	1493:1513	cell wall remodelling	1493:1513	Taken together, our data shed new light on the potential roles of cell wall remodelling for the increased freezing tolerance observed after low temperature acclimation.
33190295	7	70	from	mutant	1419:1424	arg1	absent					1399:1404	absent	1399:1404	absent	1399:1404	Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant.
33190295	7	71	with	combination	1190:1200	arg1	reconstruction					1210:1223	3D reconstruction	1207:1223	3D reconstruction of centres of rosette	1207:1245	Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant.
33190295	6	72	theme	sub-zero	1016:1023	arg1	acclimation					1025:1035	sub-zero acclimation	1016:1035	sub-zero acclimation	1016:1035	The mutant showed reduced freezing tolerance after both cold and sub-zero acclimation, compared to the Col-0 wild type, which was associated with differences in cell wall composition and structure.
34053531	1	0	theme	production	259:268	arg1	process					229:235	the sedimentation process	211:235	the sedimentation process of traditional starch production, where bacteria play a critical role in starch flocculation	211:328	Sour liquid fermentation is commonly used in the sedimentation process of traditional starch production, where bacteria play a critical role in starch flocculation.
34053531	2	1	dep	Leuconostoc	528:538	arg1	citreum					540:546	Leuconostoc citreum	528:546	Leuconostoc citreum	528:546	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	2	2	theme	Leuconostoc	683:693	arg1	citreum					695:701	Leuconostoc citreum	683:701	Leuconostoc citreum	683:701	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	2	3	theme	strong	715:720	arg1	correlation					731:741	a strong positive correlation	713:741	a strong positive correlation with starch flocculation rate (FR)	713:776	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	2	4	theme	dominant	639:646	arg1	lactis					594:599	Lactococcus lactis	582:599	Lactococcus lactis	582:599	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	2	4	theme	dominant	639:646	arg1	microorganisms					648:661	the dominant microorganisms	635:661	the dominant microorganisms in the process	635:676	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	2	4	theme	dominant	639:646	arg1	Lactobacillus					606:618	Lactobacillus	606:618	Lactobacillus	606:618	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	2	4	theme	dominant	639:646	arg1	Leuconostoc					528:538	Leuconostoc	528:538	Leuconostoc	528:538	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	2	4	theme	dominant	639:646	arg1	Leuconostoc					549:559	Leuconostoc	549:559	Leuconostoc	549:559	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	2	5	theme	starch	748:753	arg1	FR					774:775	FR	774:775	FR	774:775	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	2	5	theme	starch	748:753	arg1	rate					768:771	starch flocculation rate	748:771	starch flocculation rate (FR)	748:776	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	5	6	from	understanding	1198:1210	arg1	level					1274:1278	the species level	1262:1278	the species level	1262:1278	This study provides a comprehensive understanding of the bacterial dynamics in SPSL fermentation at the species level.
34053531	5	6	from	understanding	1198:1210	arg1	fermentation					1246:1257	SPSL fermentation	1241:1257	SPSL fermentation	1241:1257	This study provides a comprehensive understanding of the bacterial dynamics in SPSL fermentation at the species level.
34053531	8	7	theme	efficient	1676:1684	arg1	sedimentation					1693:1705	rapid and highly efficient starch sedimentation	1659:1705	rapid and highly efficient starch sedimentation	1659:1705	These results suggest that this novel bioflocculant is more suitable for modernizing the traditional SPSL fermentation process and achieving rapid and highly efficient starch sedimentation.
34053531	1	8	theme	liquid	171:176	arg1	fermentation					178:189	Sour liquid fermentation	166:189	Sour liquid fermentation	166:189	Sour liquid fermentation is commonly used in the sedimentation process of traditional starch production, where bacteria play a critical role in starch flocculation.
34053531	4	9	dep	abilities	1001:1009	arg1	FR					1025:1026	up to 55.56% FR	1012:1026	up to 55.56% FR in 20 min	1012:1036	For the first time, Leuconostoc citreum strains were reported with excellent starch-flocculating abilities (up to 55.56% FR in 20 min), which might be attributed to their ability to connect starch granules through the cell surface to form large aggregation.
34053531	5	10	from	dynamics	1229:1236	arg1	fermentation					1246:1257	SPSL fermentation	1241:1257	SPSL fermentation	1241:1257	This study provides a comprehensive understanding of the bacterial dynamics in SPSL fermentation at the species level.
34053531	6	11	theme	starch	1283:1288	arg1	yield					1303:1307	A starch flocculation yield	1281:1307	A starch flocculation yield of 93.63%	1281:1317	A starch flocculation yield of 93.63% was achieved within 1 h by using the newly discovered Leuconostoc citreum SJ-57.
34053531	6	12	theme	Leuconostoc	1373:1383	arg1	SJ-57					1393:1397	the newly discovered Leuconostoc citreum SJ-57	1352:1397	the newly discovered Leuconostoc citreum SJ-57	1352:1397	A starch flocculation yield of 93.63% was achieved within 1 h by using the newly discovered Leuconostoc citreum SJ-57.
34053531	2	13	dep	Lactobacillus	606:618	arg1	plantarum					620:628	Lactobacillus plantarum	606:628	Lactobacillus plantarum	606:628	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	2	14	theme	Lactococcus	582:592	arg1	lactis					594:599	Lactococcus lactis	582:599	Lactococcus lactis	582:599	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	2	14	theme	Lactococcus	582:592	arg1	Lactobacillus					606:618	Lactobacillus	606:618	Lactobacillus	606:618	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	2	14	theme	Lactococcus	582:592	arg1	microorganisms					648:661	the dominant microorganisms	635:661	the dominant microorganisms in the process	635:676	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	2	14	theme	Lactococcus	582:592	arg1	Leuconostoc					549:559	Leuconostoc	549:559	Leuconostoc	549:559	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	2	14	theme	Lactococcus	582:592	arg1	Leuconostoc					528:538	Leuconostoc	528:538	Leuconostoc	528:538	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	1	15	used	used	203:206	arg2	fermentation					178:189	Sour liquid fermentation	166:189	Sour liquid fermentation	166:189	Sour liquid fermentation is commonly used in the sedimentation process of traditional starch production, where bacteria play a critical role in starch flocculation.
34053531	4	16	theme	cell	1122:1125	arg1	surface					1127:1133	the cell surface	1118:1133	the cell surface to form large aggregation	1118:1159	For the first time, Leuconostoc citreum strains were reported with excellent starch-flocculating abilities (up to 55.56% FR in 20 min), which might be attributed to their ability to connect starch granules through the cell surface to form large aggregation.
34053531	7	17	theme	total	1422:1426	arg1	sedimentation					1435:1447	total starch sedimentation	1422:1447	total starch sedimentation	1422:1447	The time required for total starch sedimentation was reduced from 10 h to 4 h, compared with the traditional process.
34053531	0	18	theme	efficient	137:145	arg1	production					154:163	highly efficient starch production	130:163	highly efficient starch production	130:163	A new Leuconostoc citreum strain discovered in the traditional sweet potato sour liquid fermentation as a novel bioflocculant for highly efficient starch production.
34053531	3	19	theme	acid	799:802	arg1	LAB					815:817	LAB	815:817	LAB	815:817	In total, 75 lactic acid bacterial (LAB) strains were isolated from the SPSL, but only 7 of them caused starch flocculation.
34053531	3	19	theme	acid	799:802	arg1	strains					820:826	75 lactic acid bacterial (LAB) strains	789:826	75 lactic acid bacterial (LAB) strains	789:826	In total, 75 lactic acid bacterial (LAB) strains were isolated from the SPSL, but only 7 of them caused starch flocculation.
34053531	4	20	theme	citreum	936:942	arg1	strains					944:950	Leuconostoc citreum strains	924:950	Leuconostoc citreum strains	924:950	For the first time, Leuconostoc citreum strains were reported with excellent starch-flocculating abilities (up to 55.56% FR in 20 min), which might be attributed to their ability to connect starch granules through the cell surface to form large aggregation.
34053531	4	21	theme	starch	1094:1099	arg1	granules					1101:1108	starch granules	1094:1108	starch granules	1094:1108	For the first time, Leuconostoc citreum strains were reported with excellent starch-flocculating abilities (up to 55.56% FR in 20 min), which might be attributed to their ability to connect starch granules through the cell surface to form large aggregation.
34053531	0	22	theme	traditional	51:61	arg1	fermentation					88:99	the traditional sweet potato sour liquid fermentation	47:99	the traditional sweet potato sour liquid fermentation	47:99	A new Leuconostoc citreum strain discovered in the traditional sweet potato sour liquid fermentation as a novel bioflocculant for highly efficient starch production.
34053531	0	23	theme	Leuconostoc	6:16	arg1	strain					26:31	A new Leuconostoc citreum strain	0:31	A new Leuconostoc citreum strain	0:31	A new Leuconostoc citreum strain discovered in the traditional sweet potato sour liquid fermentation as a novel bioflocculant for highly efficient starch production.
34053531	4	24	from	FR	1025:1026	arg1	20 min					1031:1036	20 min	1031:1036	20 min	1031:1036	For the first time, Leuconostoc citreum strains were reported with excellent starch-flocculating abilities (up to 55.56% FR in 20 min), which might be attributed to their ability to connect starch granules through the cell surface to form large aggregation.
34053531	2	25	theme	real-time	484:492	arg1	sequencing					501:510	the single-molecule real-time (SMRT) sequencing	464:510	the single-molecule real-time (SMRT) sequencing	464:510	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	0	26	theme	potato	69:74	arg1	fermentation					88:99	the traditional sweet potato sour liquid fermentation	47:99	the traditional sweet potato sour liquid fermentation	47:99	A new Leuconostoc citreum strain discovered in the traditional sweet potato sour liquid fermentation as a novel bioflocculant for highly efficient starch production.
34053531	4	27	theme	first	912:916	arg1	time					918:921	the first time	908:921	the first time	908:921	For the first time, Leuconostoc citreum strains were reported with excellent starch-flocculating abilities (up to 55.56% FR in 20 min), which might be attributed to their ability to connect starch granules through the cell surface to form large aggregation.
34053531	5	28	theme	bacterial	1219:1227	arg1	dynamics					1229:1236	the bacterial dynamics	1215:1236	the bacterial dynamics in SPSL fermentation at the species level	1215:1278	This study provides a comprehensive understanding of the bacterial dynamics in SPSL fermentation at the species level.
34053531	0	29	theme	liquid	81:86	arg1	fermentation					88:99	the traditional sweet potato sour liquid fermentation	47:99	the traditional sweet potato sour liquid fermentation	47:99	A new Leuconostoc citreum strain discovered in the traditional sweet potato sour liquid fermentation as a novel bioflocculant for highly efficient starch production.
34053531	4	30	dep	%	1023:1023	arg1	55.56					1018:1022	55.56	1018:1022	55.56	1018:1022	For the first time, Leuconostoc citreum strains were reported with excellent starch-flocculating abilities (up to 55.56% FR in 20 min), which might be attributed to their ability to connect starch granules through the cell surface to form large aggregation.
34053531	3	31	theme	them	871:874	arg1	them					871:874	them	871:874	them	871:874	In total, 75 lactic acid bacterial (LAB) strains were isolated from the SPSL, but only 7 of them caused starch flocculation.
34053531	3	31	theme	them	871:874	arg1	7					866:866	7	866:866	7	866:866	In total, 75 lactic acid bacterial (LAB) strains were isolated from the SPSL, but only 7 of them caused starch flocculation.
34053531	4	32	theme	large	1143:1147	arg1	aggregation					1149:1159	large aggregation	1143:1159	large aggregation	1143:1159	For the first time, Leuconostoc citreum strains were reported with excellent starch-flocculating abilities (up to 55.56% FR in 20 min), which might be attributed to their ability to connect starch granules through the cell surface to form large aggregation.
34053531	2	33	theme	bacterial	369:377	arg1	compositions					379:390	bacterial compositions	369:390	bacterial compositions	369:390	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	6	34	theme	citreum	1385:1391	arg1	SJ-57					1393:1397	the newly discovered Leuconostoc citreum SJ-57	1352:1397	the newly discovered Leuconostoc citreum SJ-57	1352:1397	A starch flocculation yield of 93.63% was achieved within 1 h by using the newly discovered Leuconostoc citreum SJ-57.
34053531	2	35	theme	SPSL	425:428	arg1	fermentation					431:442	sweet potato sour liquid (SPSL) fermentation	399:442	sweet potato sour liquid (SPSL) fermentation	399:442	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	2	36	theme	liquid	417:422	arg1	fermentation					431:442	sweet potato sour liquid (SPSL) fermentation	399:442	sweet potato sour liquid (SPSL) fermentation	399:442	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	6	37	theme	discovered	1362:1371	arg1	SJ-57					1393:1397	the newly discovered Leuconostoc citreum SJ-57	1352:1397	the newly discovered Leuconostoc citreum SJ-57	1352:1397	A starch flocculation yield of 93.63% was achieved within 1 h by using the newly discovered Leuconostoc citreum SJ-57.
34053531	3	38	theme	starch	883:888	arg1	flocculation					890:901	starch flocculation	883:901	starch flocculation	883:901	In total, 75 lactic acid bacterial (LAB) strains were isolated from the SPSL, but only 7 of them caused starch flocculation.
34053531	2	39	theme	potato	405:410	arg1	fermentation					431:442	sweet potato sour liquid (SPSL) fermentation	399:442	sweet potato sour liquid (SPSL) fermentation	399:442	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	2	40	with	correlation	731:741	arg1	FR					774:775	FR	774:775	FR	774:775	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	2	40	with	correlation	731:741	arg1	rate					768:771	starch flocculation rate	748:771	starch flocculation rate (FR)	748:776	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	4	41	theme	starch-flocculating	981:999	arg1	abilities					1001:1009	excellent starch-flocculating abilities	971:1009	excellent starch-flocculating abilities (up to 55.56% FR in 20 min)	971:1037	For the first time, Leuconostoc citreum strains were reported with excellent starch-flocculating abilities (up to 55.56% FR in 20 min), which might be attributed to their ability to connect starch granules through the cell surface to form large aggregation.
34053531	1	42	theme	starch	310:315	arg1	flocculation					317:328	starch flocculation	310:328	starch flocculation	310:328	Sour liquid fermentation is commonly used in the sedimentation process of traditional starch production, where bacteria play a critical role in starch flocculation.
34053531	1	43	theme	sedimentation	215:227	arg1	process					229:235	the sedimentation process	211:235	the sedimentation process of traditional starch production, where bacteria play a critical role in starch flocculation	211:328	Sour liquid fermentation is commonly used in the sedimentation process of traditional starch production, where bacteria play a critical role in starch flocculation.
34053531	8	44	theme	traditional	1607:1617	arg1	process					1637:1643	the traditional SPSL fermentation process	1603:1643	the traditional SPSL fermentation process	1603:1643	These results suggest that this novel bioflocculant is more suitable for modernizing the traditional SPSL fermentation process and achieving rapid and highly efficient starch sedimentation.
34053531	2	45	dep	Leuconostoc	549:559	arg1	pseudomesenteroides					561:579	Leuconostoc pseudomesenteroides	549:579	Leuconostoc pseudomesenteroides	549:579	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	7	46	theme	starch	1428:1433	arg1	sedimentation					1435:1447	total starch sedimentation	1422:1447	total starch sedimentation	1422:1447	The time required for total starch sedimentation was reduced from 10 h to 4 h, compared with the traditional process.
34053531	8	47	theme	novel	1550:1554	arg1	bioflocculant					1556:1568	this novel bioflocculant	1545:1568	this novel bioflocculant	1545:1568	These results suggest that this novel bioflocculant is more suitable for modernizing the traditional SPSL fermentation process and achieving rapid and highly efficient starch sedimentation.
34053531	8	48	theme	fermentation	1624:1635	arg1	process					1637:1643	the traditional SPSL fermentation process	1603:1643	the traditional SPSL fermentation process	1603:1643	These results suggest that this novel bioflocculant is more suitable for modernizing the traditional SPSL fermentation process and achieving rapid and highly efficient starch sedimentation.
34053531	1	49	theme	starch	252:257	arg1	production					259:268	traditional starch production	240:268	traditional starch production	240:268	Sour liquid fermentation is commonly used in the sedimentation process of traditional starch production, where bacteria play a critical role in starch flocculation.
34053531	8	50	theme	rapid	1659:1663	arg1	sedimentation					1693:1705	rapid and highly efficient starch sedimentation	1659:1705	rapid and highly efficient starch sedimentation	1659:1705	These results suggest that this novel bioflocculant is more suitable for modernizing the traditional SPSL fermentation process and achieving rapid and highly efficient starch sedimentation.
34053531	1	51	theme	Sour	166:169	arg1	fermentation					178:189	Sour liquid fermentation	166:189	Sour liquid fermentation	166:189	Sour liquid fermentation is commonly used in the sedimentation process of traditional starch production, where bacteria play a critical role in starch flocculation.
34053531	5	52	from	fermentation	1246:1257	arg1	understanding					1198:1210	a comprehensive understanding	1182:1210	a comprehensive understanding of the bacterial dynamics in SPSL fermentation at the species level	1182:1278	This study provides a comprehensive understanding of the bacterial dynamics in SPSL fermentation at the species level.
34053531	6	53	theme	flocculation	1290:1301	arg1	yield					1303:1307	A starch flocculation yield	1281:1307	A starch flocculation yield of 93.63%	1281:1317	A starch flocculation yield of 93.63% was achieved within 1 h by using the newly discovered Leuconostoc citreum SJ-57.
34053531	8	54	theme	starch	1686:1691	arg1	sedimentation					1693:1705	rapid and highly efficient starch sedimentation	1659:1705	rapid and highly efficient starch sedimentation	1659:1705	These results suggest that this novel bioflocculant is more suitable for modernizing the traditional SPSL fermentation process and achieving rapid and highly efficient starch sedimentation.
34053531	5	55	from	level	1274:1278	arg1	understanding					1198:1210	a comprehensive understanding	1182:1210	a comprehensive understanding of the bacterial dynamics in SPSL fermentation at the species level	1182:1278	This study provides a comprehensive understanding of the bacterial dynamics in SPSL fermentation at the species level.
34053531	5	55	from	level	1274:1278	arg1	dynamics					1229:1236	the bacterial dynamics	1215:1236	the bacterial dynamics in SPSL fermentation at the species level	1215:1278	This study provides a comprehensive understanding of the bacterial dynamics in SPSL fermentation at the species level.
34053531	2	56	theme	positive	722:729	arg1	correlation					731:741	a strong positive correlation	713:741	a strong positive correlation with starch flocculation rate (FR)	713:776	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	0	57	theme	starch	147:152	arg1	production					154:163	highly efficient starch production	130:163	highly efficient starch production	130:163	A new Leuconostoc citreum strain discovered in the traditional sweet potato sour liquid fermentation as a novel bioflocculant for highly efficient starch production.
34053531	2	58	from	microorganisms	648:661	arg1	process					670:676	the process	666:676	the process	666:676	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	5	59	theme	dynamics	1229:1236	arg1	understanding					1198:1210	a comprehensive understanding	1182:1210	a comprehensive understanding of the bacterial dynamics in SPSL fermentation at the species level	1182:1278	This study provides a comprehensive understanding of the bacterial dynamics in SPSL fermentation at the species level.
34053531	2	60	theme	flocculation	755:766	arg1	FR					774:775	FR	774:775	FR	774:775	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	2	60	theme	flocculation	755:766	arg1	rate					768:771	starch flocculation rate	748:771	starch flocculation rate (FR)	748:776	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	5	61	theme	SPSL	1241:1244	arg1	fermentation					1246:1257	SPSL fermentation	1241:1257	SPSL fermentation	1241:1257	This study provides a comprehensive understanding of the bacterial dynamics in SPSL fermentation at the species level.
34053531	3	62	theme	bacterial	804:812	arg1	LAB					815:817	LAB	815:817	LAB	815:817	In total, 75 lactic acid bacterial (LAB) strains were isolated from the SPSL, but only 7 of them caused starch flocculation.
34053531	3	62	theme	bacterial	804:812	arg1	strains					820:826	75 lactic acid bacterial (LAB) strains	789:826	75 lactic acid bacterial (LAB) strains	789:826	In total, 75 lactic acid bacterial (LAB) strains were isolated from the SPSL, but only 7 of them caused starch flocculation.
34053531	0	63	theme	sweet	63:67	arg1	fermentation					88:99	the traditional sweet potato sour liquid fermentation	47:99	the traditional sweet potato sour liquid fermentation	47:99	A new Leuconostoc citreum strain discovered in the traditional sweet potato sour liquid fermentation as a novel bioflocculant for highly efficient starch production.
34053531	3	64	attach	isolated	833:840	arg2	strains					820:826	75 lactic acid bacterial (LAB) strains	789:826	75 lactic acid bacterial (LAB) strains	789:826	In total, 75 lactic acid bacterial (LAB) strains were isolated from the SPSL, but only 7 of them caused starch flocculation.
34053531	3	64	attach	isolated	833:840	arg2	LAB					815:817	LAB	815:817	LAB	815:817	In total, 75 lactic acid bacterial (LAB) strains were isolated from the SPSL, but only 7 of them caused starch flocculation.
34053531	3	64	attach	isolated	833:840	arg1	SPSL					851:854	the SPSL	847:854	the SPSL	847:854	In total, 75 lactic acid bacterial (LAB) strains were isolated from the SPSL, but only 7 of them caused starch flocculation.
34053531	4	65	theme	Leuconostoc	924:934	arg1	strains					944:950	Leuconostoc citreum strains	924:950	Leuconostoc citreum strains	924:950	For the first time, Leuconostoc citreum strains were reported with excellent starch-flocculating abilities (up to 55.56% FR in 20 min), which might be attributed to their ability to connect starch granules through the cell surface to form large aggregation.
34053531	3	66	theme	lactic	792:797	arg1	acid					799:802	lactic acid	792:802	75 lactic acid bacterial (LAB) strains	789:826	In total, 75 lactic acid bacterial (LAB) strains were isolated from the SPSL, but only 7 of them caused starch flocculation.
34053531	0	67	theme	sour	76:79	arg1	fermentation					88:99	the traditional sweet potato sour liquid fermentation	47:99	the traditional sweet potato sour liquid fermentation	47:99	A new Leuconostoc citreum strain discovered in the traditional sweet potato sour liquid fermentation as a novel bioflocculant for highly efficient starch production.
34053531	7	68	theme	traditional	1497:1507	arg1	process					1509:1515	the traditional process	1493:1515	the traditional process	1493:1515	The time required for total starch sedimentation was reduced from 10 h to 4 h, compared with the traditional process.
34053531	2	69	theme	single-molecule	468:482	arg1	sequencing					501:510	the single-molecule real-time (SMRT) sequencing	464:510	the single-molecule real-time (SMRT) sequencing	464:510	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	6	70	theme	%	1317:1317	arg1	yield					1303:1307	A starch flocculation yield	1281:1307	A starch flocculation yield of 93.63%	1281:1317	A starch flocculation yield of 93.63% was achieved within 1 h by using the newly discovered Leuconostoc citreum SJ-57.
34053531	4	71	dep	FR	1025:1026	arg1	%					1023:1023	%	1023:1023	%	1023:1023	For the first time, Leuconostoc citreum strains were reported with excellent starch-flocculating abilities (up to 55.56% FR in 20 min), which might be attributed to their ability to connect starch granules through the cell surface to form large aggregation.
34053531	4	72	dep	55.56	1018:1022	arg1	to					1015:1016	to	1015:1016	to	1015:1016	For the first time, Leuconostoc citreum strains were reported with excellent starch-flocculating abilities (up to 55.56% FR in 20 min), which might be attributed to their ability to connect starch granules through the cell surface to form large aggregation.
34053531	2	73	theme	compositions	379:390	arg1	changes					358:364	the dynamic changes	346:364	the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation	346:442	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	0	74	theme	new	2:4	arg1	strain					26:31	A new Leuconostoc citreum strain	0:31	A new Leuconostoc citreum strain	0:31	A new Leuconostoc citreum strain discovered in the traditional sweet potato sour liquid fermentation as a novel bioflocculant for highly efficient starch production.
34053531	0	75	theme	citreum	18:24	arg1	strain					26:31	A new Leuconostoc citreum strain	0:31	A new Leuconostoc citreum strain	0:31	A new Leuconostoc citreum strain discovered in the traditional sweet potato sour liquid fermentation as a novel bioflocculant for highly efficient starch production.
34053531	5	76	theme	species	1266:1272	arg1	level					1274:1278	the species level	1262:1278	the species level	1262:1278	This study provides a comprehensive understanding of the bacterial dynamics in SPSL fermentation at the species level.
34053531	2	77	theme	sour	412:415	arg1	fermentation					431:442	sweet potato sour liquid (SPSL) fermentation	399:442	sweet potato sour liquid (SPSL) fermentation	399:442	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	1	78	theme	critical	293:300	arg1	role					302:305	a critical role	291:305	a critical role	291:305	Sour liquid fermentation is commonly used in the sedimentation process of traditional starch production, where bacteria play a critical role in starch flocculation.
34053531	2	79	theme	sweet	399:403	arg1	fermentation					431:442	sweet potato sour liquid (SPSL) fermentation	399:442	sweet potato sour liquid (SPSL) fermentation	399:442	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	2	80	theme	SMRT	495:498	arg1	sequencing					501:510	the single-molecule real-time (SMRT) sequencing	464:510	the single-molecule real-time (SMRT) sequencing	464:510	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	4	81	theme	excellent	971:979	arg1	abilities					1001:1009	excellent starch-flocculating abilities	971:1009	excellent starch-flocculating abilities (up to 55.56% FR in 20 min)	971:1037	For the first time, Leuconostoc citreum strains were reported with excellent starch-flocculating abilities (up to 55.56% FR in 20 min), which might be attributed to their ability to connect starch granules through the cell surface to form large aggregation.
34053531	8	82	theme	SPSL	1619:1622	arg1	process					1637:1643	the traditional SPSL fermentation process	1603:1643	the traditional SPSL fermentation process	1603:1643	These results suggest that this novel bioflocculant is more suitable for modernizing the traditional SPSL fermentation process and achieving rapid and highly efficient starch sedimentation.
34053531	7	83	dep	4 h	1474:1476	arg1	to					1471:1472	to	1471:1472	to	1471:1472	The time required for total starch sedimentation was reduced from 10 h to 4 h, compared with the traditional process.
34053531	0	84	theme	novel	106:110	arg1	bioflocculant					112:124	a novel bioflocculant	104:124	a novel bioflocculant for highly efficient starch production	104:163	A new Leuconostoc citreum strain discovered in the traditional sweet potato sour liquid fermentation as a novel bioflocculant for highly efficient starch production.
34053531	1	85	theme	traditional	240:250	arg1	production					259:268	traditional starch production	240:268	traditional starch production	240:268	Sour liquid fermentation is commonly used in the sedimentation process of traditional starch production, where bacteria play a critical role in starch flocculation.
34053531	2	86	theme	dynamic	350:356	arg1	changes					358:364	the dynamic changes	346:364	the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation	346:442	In this study, the dynamic changes of bacterial compositions during sweet potato sour liquid (SPSL) fermentation were profiled using the single-molecule real-time (SMRT) sequencing, unveiling that Leuconostoc citreum, Leuconostoc pseudomesenteroides, Lactococcus lactis, and Lactobacillus plantarum were the dominant microorganisms in the process, and Leuconostoc citreum exhibited a strong positive correlation with starch flocculation rate (FR).
34053531	5	87	theme	comprehensive	1184:1196	arg1	understanding					1198:1210	a comprehensive understanding	1182:1210	a comprehensive understanding of the bacterial dynamics in SPSL fermentation at the species level	1182:1278	This study provides a comprehensive understanding of the bacterial dynamics in SPSL fermentation at the species level.
32307448	9	0	theme	simulated	1622:1630	arg1	fluid					1637:1641	simulated body fluid	1622:1641	simulated body fluid	1622:1641	Chemical stability and bioactivity of materials were demonstrated during in vitro studies in simulated body fluid.
32307448	1	1	theme	cement	270:275	arg1	matrix					277:282	a bone cement matrix	263:282	a bone cement matrix	263:282	PURPOSE Recently, the attention has been drawn to complex systems - biomicroconcretes composed of a bone cement matrix and resorbable granules or microspheres.
32307448	2	2	theme	material	596:603	arg1	properties					605:614	material properties	596:614	material properties	596:614	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	6	3	theme	highest	1264:1270	arg1	MPa					1300:1302	12 ± 2 MPa	1293:1302	12 ± 2 MPa	1293:1302	Biomicroconcretes consisting of sodium alginate possessed the highest mechanical strength (12 ± 2 MPa).
32307448	6	3	theme	highest	1264:1270	arg1	strength					1283:1290	the highest mechanical strength	1260:1290	the highest mechanical strength (12 ± 2 MPa)	1260:1303	Biomicroconcretes consisting of sodium alginate possessed the highest mechanical strength (12 ± 2 MPa).
32307448	7	4	theme	acid	1442:1445	arg1	addition					1410:1417	the addition	1406:1417	the addition of chitosan and acetic acid	1406:1445	It has also been found that the dissolution-precipitation reactions of the α-TCP were retarded with the addition of chitosan and acetic acid.
32307448	3	5	theme	organic	712:718	arg1	additives					720:728	organic additives	712:728	organic additives	712:728	The aim of this work was to study α-TCP-GCSD-polymer interactions and to compare the impact of organic additives on the physicochemical properties of biomicroconcretes.
32307448	10	6	theme	physicochemical	1752:1766	arg1	properties					1768:1777	promising physicochemical properties	1742:1777	promising physicochemical properties	1742:1777	CONCLUSIONS Materials containing calcium sulphate-based granules were surgically handy, possessed promising physicochemical properties and are supposed to ensure desired macroporosity as well as gradual resorption in vivo.
32307448	10	7	contain	containing	1666:1675	arg1	Materials					1656:1664	CONCLUSIONS Materials	1644:1664	CONCLUSIONS Materials containing calcium sulphate-based granules	1644:1707	CONCLUSIONS Materials containing calcium sulphate-based granules were surgically handy, possessed promising physicochemical properties and are supposed to ensure desired macroporosity as well as gradual resorption in vivo.
32307448	10	7	contain	containing	1666:1675	arg2	granules					1700:1707	calcium sulphate-based granules	1677:1707	calcium sulphate-based granules	1677:1707	CONCLUSIONS Materials containing calcium sulphate-based granules were surgically handy, possessed promising physicochemical properties and are supposed to ensure desired macroporosity as well as gradual resorption in vivo.
32307448	6	8	theme	sodium	1234:1239	arg1	alginate					1241:1248	sodium alginate	1234:1248	sodium alginate	1234:1248	Biomicroconcretes consisting of sodium alginate possessed the highest mechanical strength (12 ± 2 MPa).
32307448	4	9	theme	X-ray	867:871	arg1	METHODS					786:792	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	9	theme	X-ray	867:871	arg1	diffractometry					873:886	X-ray diffractometry	867:886	X-ray diffractometry (XRD) as well as universal testing machine (INSTRON)	867:939	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	9	theme	X-ray	867:871	arg1	XRD					889:891	XRD	889:891	XRD	889:891	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	10	theme	testing	915:921	arg1	METHODS					786:792	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	10	theme	testing	915:921	arg1	INSTRON					932:938	INSTRON	932:938	INSTRON	932:938	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	10	theme	testing	915:921	arg1	machine					923:929	universal testing machine	905:929	X-ray diffractometry (XRD) as well as universal testing machine (INSTRON)	867:939	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	3	11	theme	physicochemical	737:751	arg1	properties					753:762	the physicochemical properties	733:762	the physicochemical properties of biomicroconcretes	733:783	The aim of this work was to study α-TCP-GCSD-polymer interactions and to compare the impact of organic additives on the physicochemical properties of biomicroconcretes.
32307448	1	12	theme	complex	215:221	arg1	systems					223:229	complex systems	215:229	complex systems	215:229	PURPOSE Recently, the attention has been drawn to complex systems - biomicroconcretes composed of a bone cement matrix and resorbable granules or microspheres.
32307448	9	13	dep	in	1602:1603	arg1	vitro					1605:1609	vitro	1605:1609	vitro	1605:1609	Chemical stability and bioactivity of materials were demonstrated during in vitro studies in simulated body fluid.
32307448	5	14	theme	α-TCP	1030:1034	arg1	matrix					1036:1041	α-TCP matrix and CSD granules	1030:1058	matrix	1036:1041	RESULTS The chemical bonding between α-TCP matrix and CSD granules resulted in a compressive strength appropriate for low-load bearing applications (7-12 MPa) and clinically relevant setting times (8-33 min).
32307448	10	15	contain	possessed	1732:1740	arg1	Materials					1656:1664	CONCLUSIONS Materials	1644:1664	CONCLUSIONS Materials containing calcium sulphate-based granules	1644:1707	CONCLUSIONS Materials containing calcium sulphate-based granules were surgically handy, possessed promising physicochemical properties and are supposed to ensure desired macroporosity as well as gradual resorption in vivo.
32307448	10	15	contain	possessed	1732:1740	arg2	properties					1768:1777	promising physicochemical properties	1742:1777	promising physicochemical properties	1742:1777	CONCLUSIONS Materials containing calcium sulphate-based granules were surgically handy, possessed promising physicochemical properties and are supposed to ensure desired macroporosity as well as gradual resorption in vivo.
32307448	2	16	theme	sulphate	435:442	arg1	GCSD					464:467	GCSD	464:467	GCSD; aggregates in biomicroconcrete	464:499	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	2	16	theme	sulphate	435:442	arg1	phosphate					393:401	α-tricalcium phosphate	380:401	α-tricalcium phosphate (α-TCP; cement matrix)	380:424	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	2	16	theme	sulphate	435:442	arg1	granules					454:461	calcium sulphate dihydrate granules	427:461	calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete)	427:500	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	0	17	theme	calcium	63:69	arg1	biomicroconcretes					90:106	calcium sulphate dihydrate biomicroconcretes	63:106	calcium sulphate dihydrate biomicroconcretes containing chitosan, sodium alginate or methylcellulose	63:162	Comparative study on physicochemical properties of alpha-TCP / calcium sulphate dihydrate biomicroconcretes containing chitosan, sodium alginate or methylcellulose.
32307448	5	18	theme	low-load	1111:1118	arg1	applications					1128:1139	low-load bearing applications	1111:1139	low-load bearing applications (7-12 MPa)	1111:1150	RESULTS The chemical bonding between α-TCP matrix and CSD granules resulted in a compressive strength appropriate for low-load bearing applications (7-12 MPa) and clinically relevant setting times (8-33 min).
32307448	5	18	theme	low-load	1111:1118	arg1	MPa					1147:1149	7-12 MPa	1142:1149	7-12 MPa	1142:1149	RESULTS The chemical bonding between α-TCP matrix and CSD granules resulted in a compressive strength appropriate for low-load bearing applications (7-12 MPa) and clinically relevant setting times (8-33 min).
32307448	10	19	theme	gradual	1839:1845	arg1	resorption					1847:1856	gradual resorption	1839:1856	desired macroporosity as well as gradual resorption in vivo	1806:1864	CONCLUSIONS Materials containing calcium sulphate-based granules were surgically handy, possessed promising physicochemical properties and are supposed to ensure desired macroporosity as well as gradual resorption in vivo.
32307448	0	20	theme	dihydrate	80:88	arg1	biomicroconcretes					90:106	calcium sulphate dihydrate biomicroconcretes	63:106	calcium sulphate dihydrate biomicroconcretes containing chitosan, sodium alginate or methylcellulose	63:162	Comparative study on physicochemical properties of alpha-TCP / calcium sulphate dihydrate biomicroconcretes containing chitosan, sodium alginate or methylcellulose.
32307448	2	21	theme	sodium	534:539	arg1	chitosan					524:531	chitosan	524:531	chitosan	524:531	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	2	21	theme	sodium	534:539	arg1	alginate					541:548	sodium alginate	534:548	sodium alginate	534:548	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	1	22	dep	Recently	173:180	arg1	PURPOSE					165:171	PURPOSE	165:171	PURPOSE	165:171	PURPOSE Recently, the attention has been drawn to complex systems - biomicroconcretes composed of a bone cement matrix and resorbable granules or microspheres.
32307448	5	23	theme	compressive	1074:1084	arg1	strength					1086:1093	a compressive strength	1072:1093	a compressive strength appropriate for low-load bearing applications (7-12 MPa) and clinically relevant setting times (8-33 min)	1072:1199	RESULTS The chemical bonding between α-TCP matrix and CSD granules resulted in a compressive strength appropriate for low-load bearing applications (7-12 MPa) and clinically relevant setting times (8-33 min).
32307448	9	24	from	studies	1611:1617	arg1	fluid					1637:1641	simulated body fluid	1622:1641	simulated body fluid	1622:1641	Chemical stability and bioactivity of materials were demonstrated during in vitro studies in simulated body fluid.
32307448	4	25	theme	Gilmore	942:948	arg1	METHODS					786:792	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	25	theme	Gilmore	942:948	arg1	apparatus					950:958	Gilmore apparatus	942:958	Gilmore apparatus	942:958	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	11	26	theme	physicochemical	1954:1968	arg1	properties					1970:1979	the physicochemical properties	1950:1979	the physicochemical properties of composites	1950:1993	It has been demonstrated that the presence of CSD granules and polymers influenced the physicochemical properties of composites.
32307448	4	27	theme	electron	803:810	arg1	SEM					824:826	SEM	824:826	SEM	824:826	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	27	theme	electron	803:810	arg1	METHODS					786:792	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	27	theme	electron	803:810	arg1	microscopy					812:821	Scanning electron microscopy	794:821	Scanning electron microscopy (SEM)	794:827	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	11	28	theme	granules	1917:1924	arg1	presence					1901:1908	the presence	1897:1908	the presence of CSD granules and polymers	1897:1937	It has been demonstrated that the presence of CSD granules and polymers influenced the physicochemical properties of composites.
32307448	5	29	theme	appropriate	1095:1105	arg1	strength					1086:1093	a compressive strength	1072:1093	a compressive strength appropriate for low-load bearing applications (7-12 MPa) and clinically relevant setting times (8-33 min)	1072:1199	RESULTS The chemical bonding between α-TCP matrix and CSD granules resulted in a compressive strength appropriate for low-load bearing applications (7-12 MPa) and clinically relevant setting times (8-33 min).
32307448	9	30	theme	Chemical	1529:1536	arg1	stability					1538:1546	Chemical stability	1529:1546	Chemical stability	1529:1546	Chemical stability and bioactivity of materials were demonstrated during in vitro studies in simulated body fluid.
32307448	8	31	theme	methylcellulose	1492:1506	arg1	case					1484:1487	the case	1480:1487	the case of methylcellulose and sodium alginate	1480:1526	This effect was not observed in the case of methylcellulose and sodium alginate.
32307448	0	32	theme	Comparative	0:10	arg1	study					12:16	Comparative study	0:16	Comparative study on physicochemical properties of alpha-TCP	0:59	Comparative study on physicochemical properties of alpha-TCP / calcium sulphate dihydrate biomicroconcretes containing chitosan, sodium alginate or methylcellulose.
32307448	1	33	theme	resorbable	288:297	arg1	granules					299:306	granules	299:306	granules	299:306	PURPOSE Recently, the attention has been drawn to complex systems - biomicroconcretes composed of a bone cement matrix and resorbable granules or microspheres.
32307448	8	34	theme	sodium	1512:1517	arg1	case					1484:1487	the case	1480:1487	the case of methylcellulose and sodium alginate	1480:1526	This effect was not observed in the case of methylcellulose and sodium alginate.
32307448	10	35	theme	calcium	1677:1683	arg1	granules					1700:1707	calcium sulphate-based granules	1677:1707	calcium sulphate-based granules	1677:1707	CONCLUSIONS Materials containing calcium sulphate-based granules were surgically handy, possessed promising physicochemical properties and are supposed to ensure desired macroporosity as well as gradual resorption in vivo.
32307448	5	36	theme	setting	1176:1182	arg1	min					1196:1198	8-33 min	1191:1198	8-33 min	1191:1198	RESULTS The chemical bonding between α-TCP matrix and CSD granules resulted in a compressive strength appropriate for low-load bearing applications (7-12 MPa) and clinically relevant setting times (8-33 min).
32307448	5	36	theme	setting	1176:1182	arg1	times					1184:1188	clinically relevant setting times	1156:1188	clinically relevant setting times (8-33 min)	1156:1199	RESULTS The chemical bonding between α-TCP matrix and CSD granules resulted in a compressive strength appropriate for low-load bearing applications (7-12 MPa) and clinically relevant setting times (8-33 min).
32307448	6	37	contain	possessed	1250:1258	arg1	Biomicroconcretes					1202:1218	Biomicroconcretes	1202:1218	Biomicroconcretes consisting of sodium alginate	1202:1248	Biomicroconcretes consisting of sodium alginate possessed the highest mechanical strength (12 ± 2 MPa).
32307448	6	37	contain	possessed	1250:1258	arg2	strength					1283:1290	the highest mechanical strength	1260:1290	the highest mechanical strength (12 ± 2 MPa)	1260:1303	Biomicroconcretes consisting of sodium alginate possessed the highest mechanical strength (12 ± 2 MPa).
32307448	6	37	contain	possessed	1250:1258	arg2	MPa					1300:1302	12 ± 2 MPa	1293:1302	12 ± 2 MPa	1293:1302	Biomicroconcretes consisting of sodium alginate possessed the highest mechanical strength (12 ± 2 MPa).
32307448	4	38	theme	mercury	830:836	arg1	METHODS					786:792	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	38	theme	mercury	830:836	arg1	MIP					861:863	MIP	861:863	MIP	861:863	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	38	theme	mercury	830:836	arg1	porosimetry					848:858	mercury intrusion porosimetry	830:858	mercury intrusion porosimetry (MIP)	830:864	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	11	39	theme	polymers	1930:1937	arg1	presence					1901:1908	the presence	1897:1908	the presence of CSD granules and polymers	1897:1937	It has been demonstrated that the presence of CSD granules and polymers influenced the physicochemical properties of composites.
32307448	5	40	theme	chemical	1005:1012	arg1	bonding					1014:1020	The chemical bonding	1001:1020	The chemical bonding between α-TCP matrix and CSD granules	1001:1058	RESULTS The chemical bonding between α-TCP matrix and CSD granules resulted in a compressive strength appropriate for low-load bearing applications (7-12 MPa) and clinically relevant setting times (8-33 min).
32307448	0	41	theme	alpha-TCP	51:59	arg1	properties					37:46	physicochemical properties	21:46	physicochemical properties of alpha-TCP	21:59	Comparative study on physicochemical properties of alpha-TCP / calcium sulphate dihydrate biomicroconcretes containing chitosan, sodium alginate or methylcellulose.
32307448	10	42	theme	promising	1742:1750	arg1	properties					1768:1777	promising physicochemical properties	1742:1777	promising physicochemical properties	1742:1777	CONCLUSIONS Materials containing calcium sulphate-based granules were surgically handy, possessed promising physicochemical properties and are supposed to ensure desired macroporosity as well as gradual resorption in vivo.
32307448	2	43	theme	α-tricalcium	380:391	arg1	polymers					514:521	various polymers	506:521	various polymers (chitosan, sodium alginate, methylcellulose)	506:566	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	2	43	theme	α-tricalcium	380:391	arg1	phosphate					393:401	α-tricalcium phosphate	380:401	α-tricalcium phosphate (α-TCP; cement matrix)	380:424	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	2	43	theme	α-tricalcium	380:391	arg1	granules					454:461	calcium sulphate dihydrate granules	427:461	calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete)	427:500	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	1	44	theme	bone	265:268	arg1	matrix					277:282	a bone cement matrix	263:282	a bone cement matrix	263:282	PURPOSE Recently, the attention has been drawn to complex systems - biomicroconcretes composed of a bone cement matrix and resorbable granules or microspheres.
32307448	2	45	theme	properties	605:614	arg1	improvement					581:591	the improvement	577:591	the improvement of material properties	577:614	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	6	46	theme	mechanical	1272:1281	arg1	MPa					1300:1302	12 ± 2 MPa	1293:1302	12 ± 2 MPa	1293:1302	Biomicroconcretes consisting of sodium alginate possessed the highest mechanical strength (12 ± 2 MPa).
32307448	6	46	theme	mechanical	1272:1281	arg1	strength					1283:1290	the highest mechanical strength	1260:1290	the highest mechanical strength (12 ± 2 MPa)	1260:1303	Biomicroconcretes consisting of sodium alginate possessed the highest mechanical strength (12 ± 2 MPa).
32307448	7	47	theme	acetic	1435:1440	arg1	acid					1442:1445	acetic acid	1435:1445	acetic acid	1435:1445	It has also been found that the dissolution-precipitation reactions of the α-TCP were retarded with the addition of chitosan and acetic acid.
32307448	7	48	theme	chitosan	1422:1429	arg1	addition					1410:1417	the addition	1406:1417	the addition of chitosan and acetic acid	1406:1445	It has also been found that the dissolution-precipitation reactions of the α-TCP were retarded with the addition of chitosan and acetic acid.
32307448	3	49	theme	additives	720:728	arg1	impact					702:707	the impact	698:707	the impact of organic additives on the physicochemical properties of biomicroconcretes	698:783	The aim of this work was to study α-TCP-GCSD-polymer interactions and to compare the impact of organic additives on the physicochemical properties of biomicroconcretes.
32307448	10	50	theme	desired	1806:1812	arg1	macroporosity					1814:1826	desired macroporosity	1806:1826	desired macroporosity as well as gradual resorption in vivo	1806:1864	CONCLUSIONS Materials containing calcium sulphate-based granules were surgically handy, possessed promising physicochemical properties and are supposed to ensure desired macroporosity as well as gradual resorption in vivo.
32307448	3	51	theme	biomicroconcretes	767:783	arg1	properties					753:762	the physicochemical properties	733:762	the physicochemical properties of biomicroconcretes	733:783	The aim of this work was to study α-TCP-GCSD-polymer interactions and to compare the impact of organic additives on the physicochemical properties of biomicroconcretes.
32307448	4	52	theme	universal	905:913	arg1	METHODS					786:792	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	52	theme	universal	905:913	arg1	INSTRON					932:938	INSTRON	932:938	INSTRON	932:938	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	52	theme	universal	905:913	arg1	machine					923:929	universal testing machine	905:929	X-ray diffractometry (XRD) as well as universal testing machine (INSTRON)	867:939	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	7	53	theme	α-TCP	1381:1385	arg1	reactions					1364:1372	the dissolution-precipitation reactions	1334:1372	the dissolution-precipitation reactions of the α-TCP	1334:1385	It has also been found that the dissolution-precipitation reactions of the α-TCP were retarded with the addition of chitosan and acetic acid.
32307448	5	54	dep	RESULTS	993:999	arg1	resulted					1060:1067	resulted	1060:1067	resulted in a compressive strength appropriate for low-load bearing applications (7-12 MPa) and clinically relevant setting times (8-33 min)	1060:1199	RESULTS The chemical bonding between α-TCP matrix and CSD granules resulted in a compressive strength appropriate for low-load bearing applications (7-12 MPa) and clinically relevant setting times (8-33 min).
32307448	11	55	theme	composites	1984:1993	arg1	properties					1970:1979	the physicochemical properties	1950:1979	the physicochemical properties of composites	1950:1993	It has been demonstrated that the presence of CSD granules and polymers influenced the physicochemical properties of composites.
32307448	1	56	dep	biomicroconcretes	233:249	arg1	composed					251:258	composed	251:258	biomicroconcretes composed of a bone cement matrix and resorbable granules or microspheres	233:322	PURPOSE Recently, the attention has been drawn to complex systems - biomicroconcretes composed of a bone cement matrix and resorbable granules or microspheres.
32307448	3	57	from	impact	702:707	arg1	properties					753:762	the physicochemical properties	733:762	the physicochemical properties of biomicroconcretes	733:783	The aim of this work was to study α-TCP-GCSD-polymer interactions and to compare the impact of organic additives on the physicochemical properties of biomicroconcretes.
32307448	7	58	theme	dissolution-precipitation	1338:1362	arg1	reactions					1364:1372	the dissolution-precipitation reactions	1334:1372	the dissolution-precipitation reactions of the α-TCP	1334:1385	It has also been found that the dissolution-precipitation reactions of the α-TCP were retarded with the addition of chitosan and acetic acid.
32307448	9	59	theme	body	1632:1635	arg1	fluid					1637:1641	simulated body fluid	1622:1641	simulated body fluid	1622:1641	Chemical stability and bioactivity of materials were demonstrated during in vitro studies in simulated body fluid.
32307448	6	60	theme	±	1296:1296	arg1	MPa					1300:1302	12 ± 2 MPa	1293:1302	12 ± 2 MPa	1293:1302	Biomicroconcretes consisting of sodium alginate possessed the highest mechanical strength (12 ± 2 MPa).
32307448	6	60	theme	±	1296:1296	arg1	strength					1283:1290	the highest mechanical strength	1260:1290	the highest mechanical strength (12 ± 2 MPa)	1260:1303	Biomicroconcretes consisting of sodium alginate possessed the highest mechanical strength (12 ± 2 MPa).
32307448	0	61	theme	sulphate	71:78	arg1	biomicroconcretes					90:106	calcium sulphate dihydrate biomicroconcretes	63:106	calcium sulphate dihydrate biomicroconcretes containing chitosan, sodium alginate or methylcellulose	63:162	Comparative study on physicochemical properties of alpha-TCP / calcium sulphate dihydrate biomicroconcretes containing chitosan, sodium alginate or methylcellulose.
32307448	5	62	theme	CSD	1047:1049	arg1	granules					1051:1058	α-TCP matrix and CSD granules	1030:1058	granules	1051:1058	RESULTS The chemical bonding between α-TCP matrix and CSD granules resulted in a compressive strength appropriate for low-load bearing applications (7-12 MPa) and clinically relevant setting times (8-33 min).
32307448	2	63	theme	various	506:512	arg1	polymers					514:521	various polymers	506:521	various polymers (chitosan, sodium alginate, methylcellulose)	506:566	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	2	63	theme	various	506:512	arg1	phosphate					393:401	α-tricalcium phosphate	380:401	α-tricalcium phosphate (α-TCP; cement matrix)	380:424	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	5	64	theme	bearing	1120:1126	arg1	applications					1128:1139	low-load bearing applications	1111:1139	low-load bearing applications (7-12 MPa)	1111:1150	RESULTS The chemical bonding between α-TCP matrix and CSD granules resulted in a compressive strength appropriate for low-load bearing applications (7-12 MPa) and clinically relevant setting times (8-33 min).
32307448	5	64	theme	bearing	1120:1126	arg1	MPa					1147:1149	7-12 MPa	1142:1149	7-12 MPa	1142:1149	RESULTS The chemical bonding between α-TCP matrix and CSD granules resulted in a compressive strength appropriate for low-load bearing applications (7-12 MPa) and clinically relevant setting times (8-33 min).
32307448	4	65	dep	METHODS	786:792	arg1	SEM					824:826	SEM	824:826	SEM	824:826	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	65	dep	METHODS	786:792	arg1	MIP					861:863	MIP	861:863	MIP	861:863	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	65	dep	METHODS	786:792	arg1	apparatus					950:958	Gilmore apparatus	942:958	Gilmore apparatus	942:958	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	65	dep	METHODS	786:792	arg1	METHODS					786:792	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	65	dep	METHODS	786:792	arg1	conduct-meter					968:980	pH/ conduct-meter	964:980	pH/ conduct-meter	964:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	65	dep	METHODS	786:792	arg1	porosimetry					848:858	mercury intrusion porosimetry	830:858	mercury intrusion porosimetry (MIP)	830:864	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	65	dep	METHODS	786:792	arg1	diffractometry					873:886	X-ray diffractometry	867:886	X-ray diffractometry (XRD) as well as universal testing machine (INSTRON)	867:939	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	65	dep	METHODS	786:792	arg1	microscopy					812:821	Scanning electron microscopy	794:821	Scanning electron microscopy (SEM)	794:827	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	65	dep	METHODS	786:792	arg1	XRD					889:891	XRD	889:891	XRD	889:891	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	66	theme	pH/	964:966	arg1	METHODS					786:792	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	66	theme	pH/	964:966	arg1	conduct-meter					968:980	pH/ conduct-meter	964:980	pH/ conduct-meter	964:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	0	67	from	study	12:16	arg1	properties					37:46	physicochemical properties	21:46	physicochemical properties of alpha-TCP	21:59	Comparative study on physicochemical properties of alpha-TCP / calcium sulphate dihydrate biomicroconcretes containing chitosan, sodium alginate or methylcellulose.
32307448	2	68	from	aggregates	470:479	arg1	biomicroconcrete					484:499	biomicroconcrete	484:499	biomicroconcrete	484:499	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	10	69	theme	sulphate-based	1685:1698	arg1	granules					1700:1707	calcium sulphate-based granules	1677:1707	calcium sulphate-based granules	1677:1707	CONCLUSIONS Materials containing calcium sulphate-based granules were surgically handy, possessed promising physicochemical properties and are supposed to ensure desired macroporosity as well as gradual resorption in vivo.
32307448	0	70	theme	sodium	129:134	arg1	alginate					136:143	sodium alginate	129:143	sodium alginate	129:143	Comparative study on physicochemical properties of alpha-TCP / calcium sulphate dihydrate biomicroconcretes containing chitosan, sodium alginate or methylcellulose.
32307448	8	71	located	observed	1468:1475	arg2	effect					1453:1458	This effect	1448:1458	This effect	1448:1458	This effect was not observed in the case of methylcellulose and sodium alginate.
32307448	8	71	located	observed	1468:1475	arg1	case					1484:1487	the case	1480:1487	the case of methylcellulose and sodium alginate	1480:1526	This effect was not observed in the case of methylcellulose and sodium alginate.
32307448	2	72	dep	phosphate	393:401	arg1	α-TCP					404:408	α-TCP	404:408	α-TCP; cement matrix	404:423	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	2	73	theme	cement	411:416	arg1	matrix					418:423	cement matrix	411:423	α-TCP; cement matrix	404:423	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	4	74	used	used	987:990	arg2	porosimetry					848:858	mercury intrusion porosimetry	830:858	mercury intrusion porosimetry (MIP)	830:864	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	74	used	used	987:990	arg2	diffractometry					873:886	X-ray diffractometry	867:886	X-ray diffractometry (XRD) as well as universal testing machine (INSTRON)	867:939	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	74	used	used	987:990	arg2	microscopy					812:821	Scanning electron microscopy	794:821	Scanning electron microscopy (SEM)	794:827	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	74	used	used	987:990	arg2	METHODS					786:792	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	74	used	used	987:990	arg2	machine					923:929	universal testing machine	905:929	X-ray diffractometry (XRD) as well as universal testing machine (INSTRON)	867:939	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	74	used	used	987:990	arg2	apparatus					950:958	Gilmore apparatus	942:958	Gilmore apparatus	942:958	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	74	used	used	987:990	arg2	conduct-meter					968:980	pH/ conduct-meter	964:980	pH/ conduct-meter	964:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	2	75	dep	GCSD	464:467	arg1	aggregates					470:479	aggregates	470:479	GCSD; aggregates in biomicroconcrete	464:499	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	0	76	dep	biomicroconcretes	90:106	arg1	study					12:16	Comparative study	0:16	Comparative study on physicochemical properties of alpha-TCP	0:59	Comparative study on physicochemical properties of alpha-TCP / calcium sulphate dihydrate biomicroconcretes containing chitosan, sodium alginate or methylcellulose.
32307448	11	77	theme	CSD	1913:1915	arg1	granules					1917:1924	CSD granules	1913:1924	CSD granules	1913:1924	It has been demonstrated that the presence of CSD granules and polymers influenced the physicochemical properties of composites.
32307448	0	78	theme	physicochemical	21:35	arg1	properties					37:46	physicochemical properties	21:46	physicochemical properties of alpha-TCP	21:59	Comparative study on physicochemical properties of alpha-TCP / calcium sulphate dihydrate biomicroconcretes containing chitosan, sodium alginate or methylcellulose.
32307448	5	79	theme	relevant	1167:1174	arg1	min					1196:1198	8-33 min	1191:1198	8-33 min	1191:1198	RESULTS The chemical bonding between α-TCP matrix and CSD granules resulted in a compressive strength appropriate for low-load bearing applications (7-12 MPa) and clinically relevant setting times (8-33 min).
32307448	5	79	theme	relevant	1167:1174	arg1	times					1184:1188	clinically relevant setting times	1156:1188	clinically relevant setting times (8-33 min)	1156:1199	RESULTS The chemical bonding between α-TCP matrix and CSD granules resulted in a compressive strength appropriate for low-load bearing applications (7-12 MPa) and clinically relevant setting times (8-33 min).
32307448	2	80	theme	dihydrate	444:452	arg1	GCSD					464:467	GCSD	464:467	GCSD; aggregates in biomicroconcrete	464:499	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	2	80	theme	dihydrate	444:452	arg1	phosphate					393:401	α-tricalcium phosphate	380:401	α-tricalcium phosphate (α-TCP; cement matrix)	380:424	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	2	80	theme	dihydrate	444:452	arg1	granules					454:461	calcium sulphate dihydrate granules	427:461	calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete)	427:500	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	2	81	dep	substitutes	356:366	arg1	composed					368:375	composed	368:375	substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties	356:614	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	2	82	theme	calcium	427:433	arg1	GCSD					464:467	GCSD	464:467	GCSD; aggregates in biomicroconcrete	464:499	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	2	82	theme	calcium	427:433	arg1	phosphate					393:401	α-tricalcium phosphate	380:401	α-tricalcium phosphate (α-TCP; cement matrix)	380:424	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	2	82	theme	calcium	427:433	arg1	granules					454:461	calcium sulphate dihydrate granules	427:461	calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete)	427:500	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	9	83	theme	materials	1567:1575	arg1	bioactivity					1552:1562	bioactivity	1552:1562	bioactivity	1552:1562	Chemical stability and bioactivity of materials were demonstrated during in vitro studies in simulated body fluid.
32307448	9	83	theme	materials	1567:1575	arg1	stability					1538:1546	Chemical stability	1529:1546	Chemical stability	1529:1546	Chemical stability and bioactivity of materials were demonstrated during in vitro studies in simulated body fluid.
32307448	3	84	theme	work	633:636	arg1	aim					621:623	The aim	617:623	The aim of this work	617:636	The aim of this work was to study α-TCP-GCSD-polymer interactions and to compare the impact of organic additives on the physicochemical properties of biomicroconcretes.
32307448	10	85	theme	CONCLUSIONS	1644:1654	arg1	Materials					1656:1664	CONCLUSIONS Materials	1644:1664	CONCLUSIONS Materials containing calcium sulphate-based granules	1644:1707	CONCLUSIONS Materials containing calcium sulphate-based granules were surgically handy, possessed promising physicochemical properties and are supposed to ensure desired macroporosity as well as gradual resorption in vivo.
32307448	4	86	theme	intrusion	838:846	arg1	METHODS					786:792	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	86	theme	intrusion	838:846	arg1	MIP					861:863	MIP	861:863	MIP	861:863	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	86	theme	intrusion	838:846	arg1	porosimetry					848:858	mercury intrusion porosimetry	830:858	mercury intrusion porosimetry (MIP)	830:864	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	0	87	contain	containing	108:117	arg2	alginate					136:143	sodium alginate	129:143	sodium alginate	129:143	Comparative study on physicochemical properties of alpha-TCP / calcium sulphate dihydrate biomicroconcretes containing chitosan, sodium alginate or methylcellulose.
32307448	0	87	contain	containing	108:117	arg2	chitosan					119:126	chitosan	119:126	chitosan	119:126	Comparative study on physicochemical properties of alpha-TCP / calcium sulphate dihydrate biomicroconcretes containing chitosan, sodium alginate or methylcellulose.
32307448	0	87	contain	containing	108:117	arg1	biomicroconcretes					90:106	calcium sulphate dihydrate biomicroconcretes	63:106	calcium sulphate dihydrate biomicroconcretes containing chitosan, sodium alginate or methylcellulose	63:162	Comparative study on physicochemical properties of alpha-TCP / calcium sulphate dihydrate biomicroconcretes containing chitosan, sodium alginate or methylcellulose.
32307448	0	87	contain	containing	108:117	arg2	methylcellulose					148:162	methylcellulose	148:162	methylcellulose	148:162	Comparative study on physicochemical properties of alpha-TCP / calcium sulphate dihydrate biomicroconcretes containing chitosan, sodium alginate or methylcellulose.
32307448	2	88	dep	polymers	514:521	arg1	methylcellulose					551:565	methylcellulose	551:565	methylcellulose	551:565	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	2	88	dep	polymers	514:521	arg1	chitosan					524:531	chitosan	524:531	chitosan	524:531	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	2	88	dep	polymers	514:521	arg1	alginate					541:548	sodium alginate	534:548	sodium alginate	534:548	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	9	89	theme	in	1602:1603	arg1	studies					1611:1617	in vitro studies	1602:1617	in vitro studies in simulated body fluid	1602:1641	Chemical stability and bioactivity of materials were demonstrated during in vitro studies in simulated body fluid.
32307448	4	90	theme	Scanning	794:801	arg1	SEM					824:826	SEM	824:826	SEM	824:826	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	90	theme	Scanning	794:801	arg1	METHODS					786:792	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter	786:980	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	4	90	theme	Scanning	794:801	arg1	microscopy					812:821	Scanning electron microscopy	794:821	Scanning electron microscopy (SEM)	794:827	METHODS Scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP), X-ray diffractometry (XRD) as well as universal testing machine (INSTRON), Gilmore apparatus and pH/ conduct-meter were used.
32307448	3	91	theme	α-TCP-GCSD-polymer	651:668	arg1	interactions					670:681	α-TCP-GCSD-polymer interactions	651:681	α-TCP-GCSD-polymer interactions	651:681	The aim of this work was to study α-TCP-GCSD-polymer interactions and to compare the impact of organic additives on the physicochemical properties of biomicroconcretes.
32307448	2	92	dep	α-TCP	404:408	arg1	matrix					418:423	cement matrix	411:423	α-TCP; cement matrix	404:423	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	2	93	theme	novel	345:349	arg1	bone					351:354	novel bone	345:354	novel bone	345:354	This paper presents novel bone substitutes composed of α-tricalcium phosphate (α-TCP; cement matrix), calcium sulphate dihydrate granules (GCSD; aggregates in biomicroconcrete) and various polymers (chitosan, sodium alginate, methylcellulose) used for the improvement of material properties.
32307448	8	94	dep	methylcellulose	1492:1506	arg1	alginate					1519:1526	alginate	1519:1526	alginate	1519:1526	This effect was not observed in the case of methylcellulose and sodium alginate.
33938391	7	0	theme	3-week	1357:1362	arg1	intervention					1364:1375	the 3-week intervention	1353:1375	the 3-week intervention	1353:1375	OTUs corresponding to the species Bifidobacterium longum, Bifidobacterium adolescentis and Ruminococcus gnavus were not present in the control after the 3-week intervention, but were shared among the donor and specific disaccharide groups, indicating that their survival is dependent on disaccharide supplementation.
33938391	2	1	theme	infant	282:287	arg1	transplantation					295:309	infant fecal transplantation	282:309	infant fecal transplantation	282:309	A humanized mouse model generated by infant fecal transplantation was utilized here to evaluate the impact of fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto-N-biose (LNB) and galacto-N-biose on the fecal microbiota and host-microbiota interactions.
33938391	9	2	theme	large	1814:1818	arg1	intestine					1820:1828	the large intestine	1810:1828	the large intestine tissue	1810:1835	3FN also down-regulated the pro-inflammatory cytokine TNF-α and up-regulated the anti-inflammatory cytokines IL-10 and IL-13, and the Toll-like receptor 2 in the large intestine tissue.
33938391	3	3	theme	bacterial	550:558	arg1	genera					560:565	certain bacterial genera	542:565	certain bacterial genera	542:565	16S rRNA amplicon sequencing showed that certain bacterial genera significantly increased (Ruminococcus and Oscillospira) or decreased (Eubacterium and Clostridium) in all disaccharide-supplemented groups.
33938391	9	4	dep	cytokines	1751:1759	arg1	cytokines					1751:1759	the anti-inflammatory cytokines	1729:1759	the anti-inflammatory cytokines IL-10 and IL-13	1729:1775	3FN also down-regulated the pro-inflammatory cytokine TNF-α and up-regulated the anti-inflammatory cytokines IL-10 and IL-13, and the Toll-like receptor 2 in the large intestine tissue.
33938391	9	4	dep	cytokines	1751:1759	arg1	IL-13					1771:1775	IL-13	1771:1775	IL-13	1771:1775	3FN also down-regulated the pro-inflammatory cytokine TNF-α and up-regulated the anti-inflammatory cytokines IL-10 and IL-13, and the Toll-like receptor 2 in the large intestine tissue.
33938391	9	4	dep	cytokines	1751:1759	arg1	IL-10					1761:1765	IL-10	1761:1765	IL-10	1761:1765	3FN also down-regulated the pro-inflammatory cytokine TNF-α and up-regulated the anti-inflammatory cytokines IL-10 and IL-13, and the Toll-like receptor 2 in the large intestine tissue.
33938391	8	5	from	butyrate	1570:1577	arg1	colon					1598:1602	the colon	1594:1602	the colon	1594:1602	The 3FN-feeding group showed increased levels of butyrate and acetate in the colon, and decreased levels of serum HDL-cholesterol.
33938391	3	6	dep	decreased	626:634	arg1	Eubacterium					637:647	Eubacterium	637:647	Eubacterium	637:647	16S rRNA amplicon sequencing showed that certain bacterial genera significantly increased (Ruminococcus and Oscillospira) or decreased (Eubacterium and Clostridium) in all disaccharide-supplemented groups.
33938391	3	6	dep	decreased	626:634	arg1	Clostridium					653:663	Clostridium	653:663	Clostridium	653:663	16S rRNA amplicon sequencing showed that certain bacterial genera significantly increased (Ruminococcus and Oscillospira) or decreased (Eubacterium and Clostridium) in all disaccharide-supplemented groups.
33938391	7	7	dep	species	1230:1236	arg1	longum					1254:1259	Bifidobacterium longum	1238:1259	Bifidobacterium longum	1238:1259	OTUs corresponding to the species Bifidobacterium longum, Bifidobacterium adolescentis and Ruminococcus gnavus were not present in the control after the 3-week intervention, but were shared among the donor and specific disaccharide groups, indicating that their survival is dependent on disaccharide supplementation.
33938391	7	7	dep	species	1230:1236	arg1	adolescentis					1278:1289	Bifidobacterium adolescentis	1262:1289	Bifidobacterium adolescentis	1262:1289	OTUs corresponding to the species Bifidobacterium longum, Bifidobacterium adolescentis and Ruminococcus gnavus were not present in the control after the 3-week intervention, but were shared among the donor and specific disaccharide groups, indicating that their survival is dependent on disaccharide supplementation.
33938391	7	7	dep	species	1230:1236	arg1	gnavus					1308:1313	Ruminococcus gnavus	1295:1313	Ruminococcus gnavus	1295:1313	OTUs corresponding to the species Bifidobacterium longum, Bifidobacterium adolescentis and Ruminococcus gnavus were not present in the control after the 3-week intervention, but were shared among the donor and specific disaccharide groups, indicating that their survival is dependent on disaccharide supplementation.
33938391	10	8	theme	beneficial	1920:1929	arg1	shifts					1945:1950	beneficial compositional shifts	1920:1950	beneficial compositional shifts of the gut microbiota	1920:1972	The present study revealed that the four disaccharides show efficacy in producing beneficial compositional shifts of the gut microbiota and in addition, the 3FN demonstrated physiological and immunomodulatory roles.
33938391	8	9	from	levels	1560:1565	arg1	colon					1598:1602	the colon	1594:1602	the colon	1594:1602	The 3FN-feeding group showed increased levels of butyrate and acetate in the colon, and decreased levels of serum HDL-cholesterol.
33938391	5	10	theme	abundance	947:955	arg1	increment					921:929	An increment	918:929	An increment of the relative abundance of Coprococcus genus	918:976	An increment of the relative abundance of Coprococcus genus was only observed with 3FN.
33938391	10	11	theme	microbiota	1963:1972	arg1	shifts					1945:1950	beneficial compositional shifts	1920:1950	beneficial compositional shifts of the gut microbiota	1920:1972	The present study revealed that the four disaccharides show efficacy in producing beneficial compositional shifts of the gut microbiota and in addition, the 3FN demonstrated physiological and immunomodulatory roles.
33938391	5	12	theme	Coprococcus	960:970	arg1	genus					972:976	Coprococcus genus	960:976	Coprococcus genus	960:976	An increment of the relative abundance of Coprococcus genus was only observed with 3FN.
33938391	6	13	theme	relative	1047:1054	arg1	abundance					1056:1064	the relative abundance	1043:1064	the relative abundance of Bifidobacterium	1043:1083	As well, LNB significantly increased the relative abundance of Bifidobacterium, whereas the absolute levels of this genus, as measured by quantitative real-time PCR, did not significantly increase.
33938391	7	14	attach	present	1324:1330	arg1	control					1339:1345	the control	1335:1345	the control after the 3-week intervention	1335:1375	OTUs corresponding to the species Bifidobacterium longum, Bifidobacterium adolescentis and Ruminococcus gnavus were not present in the control after the 3-week intervention, but were shared among the donor and specific disaccharide groups, indicating that their survival is dependent on disaccharide supplementation.
33938391	7	14	attach	present	1324:1330	arg2	OTUs					1204:1207	OTUs	1204:1207	OTUs corresponding to the species Bifidobacterium longum, Bifidobacterium adolescentis and Ruminococcus gnavus	1204:1313	OTUs corresponding to the species Bifidobacterium longum, Bifidobacterium adolescentis and Ruminococcus gnavus were not present in the control after the 3-week intervention, but were shared among the donor and specific disaccharide groups, indicating that their survival is dependent on disaccharide supplementation.
33938391	7	15	from	control	1339:1345	arg1	present					1324:1330	present	1324:1330	present	1324:1330	OTUs corresponding to the species Bifidobacterium longum, Bifidobacterium adolescentis and Ruminococcus gnavus were not present in the control after the 3-week intervention, but were shared among the donor and specific disaccharide groups, indicating that their survival is dependent on disaccharide supplementation.
33938391	2	16	theme	mouse	257:261	arg1	model					263:267	A humanized mouse model	245:267	A humanized mouse model generated by infant fecal transplantation	245:309	A humanized mouse model generated by infant fecal transplantation was utilized here to evaluate the impact of fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto-N-biose (LNB) and galacto-N-biose on the fecal microbiota and host-microbiota interactions.
33938391	0	17	theme	microbiota	11:20	arg1	modulation					22:31	Infant gut microbiota modulation	0:31	Infant gut microbiota modulation by human milk	0:45	Infant gut microbiota modulation by human milk disaccharides in humanized microbiome mice.
33938391	1	18	theme	structures	140:149	arg1	diversity					127:135	a unique diversity	118:135	a unique diversity of structures that suggest different mechanisms by which they may affect the infant microbiome development	118:242	Human milk glycans present a unique diversity of structures that suggest different mechanisms by which they may affect the infant microbiome development.
33938391	0	19	theme	microbiome	74:83	arg1	mice					85:88	humanized microbiome mice	64:88	humanized microbiome mice	64:88	Infant gut microbiota modulation by human milk disaccharides in humanized microbiome mice.
33938391	8	20	theme	serum	1629:1633	arg1	HDL-cholesterol					1635:1649	serum HDL-cholesterol	1629:1649	serum HDL-cholesterol	1629:1649	The 3FN-feeding group showed increased levels of butyrate and acetate in the colon, and decreased levels of serum HDL-cholesterol.
33938391	9	21	theme	pro-inflammatory	1680:1695	arg1	cytokine					1697:1704	the pro-inflammatory cytokine	1676:1704	the pro-inflammatory cytokine TNF-α	1676:1710	3FN also down-regulated the pro-inflammatory cytokine TNF-α and up-regulated the anti-inflammatory cytokines IL-10 and IL-13, and the Toll-like receptor 2 in the large intestine tissue.
33938391	7	22	theme	disaccharide	1423:1434	arg1	groups					1436:1441	the donor and specific disaccharide groups	1400:1441	groups	1436:1441	OTUs corresponding to the species Bifidobacterium longum, Bifidobacterium adolescentis and Ruminococcus gnavus were not present in the control after the 3-week intervention, but were shared among the donor and specific disaccharide groups, indicating that their survival is dependent on disaccharide supplementation.
33938391	4	23	theme	genus	878:882	arg1	disappearance					849:861	the disappearance	845:861	the disappearance of Akkermansia genus in both fucosyl-oligosaccharides	845:915	Interestingly, cluster analysis differentiates the consumption of fucosyl-oligosaccharides from galactosyl-oligosaccharides, highlighting the disappearance of Akkermansia genus in both fucosyl-oligosaccharides.
33938391	8	24	from	colon	1598:1602	arg1	levels					1560:1565	increased levels	1550:1565	increased levels of butyrate and acetate in the colon	1550:1602	The 3FN-feeding group showed increased levels of butyrate and acetate in the colon, and decreased levels of serum HDL-cholesterol.
33938391	8	24	from	colon	1598:1602	arg1	levels					1619:1624	decreased levels	1609:1624	decreased levels of serum HDL-cholesterol	1609:1649	The 3FN-feeding group showed increased levels of butyrate and acetate in the colon, and decreased levels of serum HDL-cholesterol.
33938391	0	25	theme	Infant	0:5	arg1	modulation					22:31	Infant gut microbiota modulation	0:31	Infant gut microbiota modulation by human milk	0:45	Infant gut microbiota modulation by human milk disaccharides in humanized microbiome mice.
33938391	1	26	theme	Human	91:95	arg1	glycans					102:108	Human milk glycans	91:108	Human milk glycans	91:108	Human milk glycans present a unique diversity of structures that suggest different mechanisms by which they may affect the infant microbiome development.
33938391	10	27	theme	physiological	2012:2024	arg1	roles					2047:2051	physiological and immunomodulatory roles	2012:2051	physiological and immunomodulatory roles	2012:2051	The present study revealed that the four disaccharides show efficacy in producing beneficial compositional shifts of the gut microbiota and in addition, the 3FN demonstrated physiological and immunomodulatory roles.
33938391	2	28	theme	galacto-N-biose	427:441	arg1	impact					345:350	the impact	341:350	the impact of fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto-N-biose (LNB) and galacto-N-biose on the fecal microbiota and host-microbiota interactions	341:498	A humanized mouse model generated by infant fecal transplantation was utilized here to evaluate the impact of fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto-N-biose (LNB) and galacto-N-biose on the fecal microbiota and host-microbiota interactions.
33938391	8	29	from	levels	1619:1624	arg1	colon					1598:1602	the colon	1594:1602	the colon	1594:1602	The 3FN-feeding group showed increased levels of butyrate and acetate in the colon, and decreased levels of serum HDL-cholesterol.
33938391	9	30	theme	anti-inflammatory	1733:1749	arg1	cytokines					1751:1759	the anti-inflammatory cytokines	1729:1759	the anti-inflammatory cytokines IL-10 and IL-13	1729:1775	3FN also down-regulated the pro-inflammatory cytokine TNF-α and up-regulated the anti-inflammatory cytokines IL-10 and IL-13, and the Toll-like receptor 2 in the large intestine tissue.
33938391	9	30	theme	anti-inflammatory	1733:1749	arg1	IL-13					1771:1775	IL-13	1771:1775	IL-13	1771:1775	3FN also down-regulated the pro-inflammatory cytokine TNF-α and up-regulated the anti-inflammatory cytokines IL-10 and IL-13, and the Toll-like receptor 2 in the large intestine tissue.
33938391	9	30	theme	anti-inflammatory	1733:1749	arg1	IL-10					1761:1765	IL-10	1761:1765	IL-10	1761:1765	3FN also down-regulated the pro-inflammatory cytokine TNF-α and up-regulated the anti-inflammatory cytokines IL-10 and IL-13, and the Toll-like receptor 2 in the large intestine tissue.
33938391	9	31	from	cytokines	1751:1759	arg1	tissue					1830:1835	the large intestine tissue	1810:1835	the large intestine tissue	1810:1835	3FN also down-regulated the pro-inflammatory cytokine TNF-α and up-regulated the anti-inflammatory cytokines IL-10 and IL-13, and the Toll-like receptor 2 in the large intestine tissue.
33938391	6	32	theme	quantitative	1144:1155	arg1	PCR					1167:1169	quantitative real-time PCR	1144:1169	quantitative real-time PCR	1144:1169	As well, LNB significantly increased the relative abundance of Bifidobacterium, whereas the absolute levels of this genus, as measured by quantitative real-time PCR, did not significantly increase.
33938391	2	33	theme	host-microbiota	471:485	arg1	interactions					487:498	the fecal microbiota and host-microbiota interactions	446:498	interactions	487:498	A humanized mouse model generated by infant fecal transplantation was utilized here to evaluate the impact of fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto-N-biose (LNB) and galacto-N-biose on the fecal microbiota and host-microbiota interactions.
33938391	3	34	theme	disaccharide-supplemented	673:697	arg1	groups					699:704	all disaccharide-supplemented groups	669:704	all disaccharide-supplemented groups	669:704	16S rRNA amplicon sequencing showed that certain bacterial genera significantly increased (Ruminococcus and Oscillospira) or decreased (Eubacterium and Clostridium) in all disaccharide-supplemented groups.
33938391	8	35	theme	3FN-feeding	1525:1535	arg1	group					1537:1541	The 3FN-feeding group	1521:1541	The 3FN-feeding group	1521:1541	The 3FN-feeding group showed increased levels of butyrate and acetate in the colon, and decreased levels of serum HDL-cholesterol.
33938391	5	36	theme	genus	972:976	arg1	abundance					947:955	the relative abundance	934:955	the relative abundance of Coprococcus genus	934:976	An increment of the relative abundance of Coprococcus genus was only observed with 3FN.
33938391	7	37	located	present	1324:1330	arg1	control					1339:1345	the control	1335:1345	the control after the 3-week intervention	1335:1375	OTUs corresponding to the species Bifidobacterium longum, Bifidobacterium adolescentis and Ruminococcus gnavus were not present in the control after the 3-week intervention, but were shared among the donor and specific disaccharide groups, indicating that their survival is dependent on disaccharide supplementation.
33938391	7	37	located	present	1324:1330	arg2	OTUs					1204:1207	OTUs	1204:1207	OTUs corresponding to the species Bifidobacterium longum, Bifidobacterium adolescentis and Ruminococcus gnavus	1204:1313	OTUs corresponding to the species Bifidobacterium longum, Bifidobacterium adolescentis and Ruminococcus gnavus were not present in the control after the 3-week intervention, but were shared among the donor and specific disaccharide groups, indicating that their survival is dependent on disaccharide supplementation.
33938391	3	38	theme	16S	501:503	arg1	sequencing					519:528	16S rRNA amplicon sequencing	501:528	16S rRNA amplicon sequencing	501:528	16S rRNA amplicon sequencing showed that certain bacterial genera significantly increased (Ruminococcus and Oscillospira) or decreased (Eubacterium and Clostridium) in all disaccharide-supplemented groups.
33938391	10	39	theme	present	1842:1848	arg1	study					1850:1854	The present study	1838:1854	The present study	1838:1854	The present study revealed that the four disaccharides show efficacy in producing beneficial compositional shifts of the gut microbiota and in addition, the 3FN demonstrated physiological and immunomodulatory roles.
33938391	1	40	theme	infant	214:219	arg1	development					232:242	the infant microbiome development	210:242	the infant microbiome development	210:242	Human milk glycans present a unique diversity of structures that suggest different mechanisms by which they may affect the infant microbiome development.
33938391	2	41	used	utilized	315:322	arg2	model					263:267	A humanized mouse model	245:267	A humanized mouse model generated by infant fecal transplantation	245:309	A humanized mouse model generated by infant fecal transplantation was utilized here to evaluate the impact of fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto-N-biose (LNB) and galacto-N-biose on the fecal microbiota and host-microbiota interactions.
33938391	10	42	theme	immunomodulatory	2030:2045	arg1	roles					2047:2051	physiological and immunomodulatory roles	2012:2051	physiological and immunomodulatory roles	2012:2051	The present study revealed that the four disaccharides show efficacy in producing beneficial compositional shifts of the gut microbiota and in addition, the 3FN demonstrated physiological and immunomodulatory roles.
33938391	8	43	theme	butyrate	1570:1577	arg1	levels					1560:1565	increased levels	1550:1565	increased levels of butyrate and acetate in the colon	1550:1602	The 3FN-feeding group showed increased levels of butyrate and acetate in the colon, and decreased levels of serum HDL-cholesterol.
33938391	8	43	theme	butyrate	1570:1577	arg1	levels					1619:1624	decreased levels	1609:1624	decreased levels of serum HDL-cholesterol	1609:1649	The 3FN-feeding group showed increased levels of butyrate and acetate in the colon, and decreased levels of serum HDL-cholesterol.
33938391	4	44	from	consumption	758:768	arg1	galactosyl-oligosaccharides					803:829	galactosyl-oligosaccharides	803:829	galactosyl-oligosaccharides	803:829	Interestingly, cluster analysis differentiates the consumption of fucosyl-oligosaccharides from galactosyl-oligosaccharides, highlighting the disappearance of Akkermansia genus in both fucosyl-oligosaccharides.
33938391	9	45	theme	Toll-like	1786:1794	arg1	receptor					1796:1803	the Toll-like receptor 2	1782:1805	the Toll-like receptor 2 in the large intestine tissue	1782:1835	3FN also down-regulated the pro-inflammatory cytokine TNF-α and up-regulated the anti-inflammatory cytokines IL-10 and IL-13, and the Toll-like receptor 2 in the large intestine tissue.
33938391	2	46	theme	fucosyl-α1,3-GlcNAc	355:373	arg1	impact					345:350	the impact	341:350	the impact of fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto-N-biose (LNB) and galacto-N-biose on the fecal microbiota and host-microbiota interactions	341:498	A humanized mouse model generated by infant fecal transplantation was utilized here to evaluate the impact of fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto-N-biose (LNB) and galacto-N-biose on the fecal microbiota and host-microbiota interactions.
33938391	2	47	theme	fecal	289:293	arg1	transplantation					295:309	infant fecal transplantation	282:309	infant fecal transplantation	282:309	A humanized mouse model generated by infant fecal transplantation was utilized here to evaluate the impact of fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto-N-biose (LNB) and galacto-N-biose on the fecal microbiota and host-microbiota interactions.
33938391	2	48	from	impact	345:350	arg1	microbiota					456:465	the fecal microbiota and host-microbiota interactions	446:498	microbiota	456:465	A humanized mouse model generated by infant fecal transplantation was utilized here to evaluate the impact of fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto-N-biose (LNB) and galacto-N-biose on the fecal microbiota and host-microbiota interactions.
33938391	2	48	from	impact	345:350	arg1	interactions					487:498	the fecal microbiota and host-microbiota interactions	446:498	interactions	487:498	A humanized mouse model generated by infant fecal transplantation was utilized here to evaluate the impact of fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto-N-biose (LNB) and galacto-N-biose on the fecal microbiota and host-microbiota interactions.
33938391	3	49	theme	certain	542:548	arg1	genera					560:565	certain bacterial genera	542:565	certain bacterial genera	542:565	16S rRNA amplicon sequencing showed that certain bacterial genera significantly increased (Ruminococcus and Oscillospira) or decreased (Eubacterium and Clostridium) in all disaccharide-supplemented groups.
33938391	1	50	theme	different	164:172	arg1	mechanisms					174:183	different mechanisms	164:183	different mechanisms by which they may affect the infant microbiome development	164:242	Human milk glycans present a unique diversity of structures that suggest different mechanisms by which they may affect the infant microbiome development.
33938391	9	51	theme	intestine	1820:1828	arg1	tissue					1830:1835	the large intestine tissue	1810:1835	the large intestine tissue	1810:1835	3FN also down-regulated the pro-inflammatory cytokine TNF-α and up-regulated the anti-inflammatory cytokines IL-10 and IL-13, and the Toll-like receptor 2 in the large intestine tissue.
33938391	10	52	theme	compositional	1931:1943	arg1	shifts					1945:1950	beneficial compositional shifts	1920:1950	beneficial compositional shifts of the gut microbiota	1920:1972	The present study revealed that the four disaccharides show efficacy in producing beneficial compositional shifts of the gut microbiota and in addition, the 3FN demonstrated physiological and immunomodulatory roles.
33938391	5	53	theme	relative	938:945	arg1	abundance					947:955	the relative abundance	934:955	the relative abundance of Coprococcus genus	934:976	An increment of the relative abundance of Coprococcus genus was only observed with 3FN.
33938391	6	54	theme	Bifidobacterium	1069:1083	arg1	abundance					1056:1064	the relative abundance	1043:1064	the relative abundance of Bifidobacterium	1043:1083	As well, LNB significantly increased the relative abundance of Bifidobacterium, whereas the absolute levels of this genus, as measured by quantitative real-time PCR, did not significantly increase.
33938391	8	55	theme	acetate	1583:1589	arg1	levels					1560:1565	increased levels	1550:1565	increased levels of butyrate and acetate in the colon	1550:1602	The 3FN-feeding group showed increased levels of butyrate and acetate in the colon, and decreased levels of serum HDL-cholesterol.
33938391	8	55	theme	acetate	1583:1589	arg1	levels					1619:1624	decreased levels	1609:1624	decreased levels of serum HDL-cholesterol	1609:1649	The 3FN-feeding group showed increased levels of butyrate and acetate in the colon, and decreased levels of serum HDL-cholesterol.
33938391	3	56	theme	amplicon	510:517	arg1	sequencing					519:528	16S rRNA amplicon sequencing	501:528	16S rRNA amplicon sequencing	501:528	16S rRNA amplicon sequencing showed that certain bacterial genera significantly increased (Ruminococcus and Oscillospira) or decreased (Eubacterium and Clostridium) in all disaccharide-supplemented groups.
33938391	7	57	from	present	1324:1330	arg1	control					1339:1345	the control	1335:1345	the control after the 3-week intervention	1335:1375	OTUs corresponding to the species Bifidobacterium longum, Bifidobacterium adolescentis and Ruminococcus gnavus were not present in the control after the 3-week intervention, but were shared among the donor and specific disaccharide groups, indicating that their survival is dependent on disaccharide supplementation.
33938391	10	58	theme	gut	1959:1961	arg1	microbiota					1963:1972	the gut microbiota	1955:1972	the gut microbiota	1955:1972	The present study revealed that the four disaccharides show efficacy in producing beneficial compositional shifts of the gut microbiota and in addition, the 3FN demonstrated physiological and immunomodulatory roles.
33938391	6	59	dep	levels	1107:1112	arg1	measured					1132:1139	measured	1132:1139	measured by quantitative real-time PCR	1132:1169	As well, LNB significantly increased the relative abundance of Bifidobacterium, whereas the absolute levels of this genus, as measured by quantitative real-time PCR, did not significantly increase.
33938391	9	60	from	receptor	1796:1803	arg1	tissue					1830:1835	the large intestine tissue	1810:1835	the large intestine tissue	1810:1835	3FN also down-regulated the pro-inflammatory cytokine TNF-α and up-regulated the anti-inflammatory cytokines IL-10 and IL-13, and the Toll-like receptor 2 in the large intestine tissue.
33938391	7	61	theme	disaccharide	1491:1502	arg1	supplementation					1504:1518	disaccharide supplementation	1491:1518	disaccharide supplementation	1491:1518	OTUs corresponding to the species Bifidobacterium longum, Bifidobacterium adolescentis and Ruminococcus gnavus were not present in the control after the 3-week intervention, but were shared among the donor and specific disaccharide groups, indicating that their survival is dependent on disaccharide supplementation.
33938391	1	62	theme	unique	120:125	arg1	diversity					127:135	a unique diversity	118:135	a unique diversity of structures that suggest different mechanisms by which they may affect the infant microbiome development	118:242	Human milk glycans present a unique diversity of structures that suggest different mechanisms by which they may affect the infant microbiome development.
33938391	8	63	theme	decreased	1609:1617	arg1	levels					1619:1624	decreased levels	1609:1624	decreased levels of serum HDL-cholesterol	1609:1649	The 3FN-feeding group showed increased levels of butyrate and acetate in the colon, and decreased levels of serum HDL-cholesterol.
33938391	4	64	theme	fucosyl-oligosaccharides	773:796	arg1	consumption					758:768	the consumption	754:768	the consumption of fucosyl-oligosaccharides from galactosyl-oligosaccharides	754:829	Interestingly, cluster analysis differentiates the consumption of fucosyl-oligosaccharides from galactosyl-oligosaccharides, highlighting the disappearance of Akkermansia genus in both fucosyl-oligosaccharides.
33938391	0	65	theme	humanized	64:72	arg1	mice					85:88	humanized microbiome mice	64:88	humanized microbiome mice	64:88	Infant gut microbiota modulation by human milk disaccharides in humanized microbiome mice.
33938391	2	66	theme	humanized	247:255	arg1	model					263:267	A humanized mouse model	245:267	A humanized mouse model generated by infant fecal transplantation	245:309	A humanized mouse model generated by infant fecal transplantation was utilized here to evaluate the impact of fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto-N-biose (LNB) and galacto-N-biose on the fecal microbiota and host-microbiota interactions.
33938391	3	67	dep	increased	581:589	arg1	Oscillospira					609:620	Oscillospira	609:620	Oscillospira	609:620	16S rRNA amplicon sequencing showed that certain bacterial genera significantly increased (Ruminococcus and Oscillospira) or decreased (Eubacterium and Clostridium) in all disaccharide-supplemented groups.
33938391	3	67	dep	increased	581:589	arg1	Ruminococcus					592:603	Ruminococcus	592:603	Ruminococcus	592:603	16S rRNA amplicon sequencing showed that certain bacterial genera significantly increased (Ruminococcus and Oscillospira) or decreased (Eubacterium and Clostridium) in all disaccharide-supplemented groups.
33938391	6	68	theme	genus	1122:1126	arg1	levels					1107:1112	the absolute levels	1094:1112	the absolute levels	1094:1112	As well, LNB significantly increased the relative abundance of Bifidobacterium, whereas the absolute levels of this genus, as measured by quantitative real-time PCR, did not significantly increase.
33938391	4	69	theme	cluster	722:728	arg1	analysis					730:737	cluster analysis	722:737	cluster analysis	722:737	Interestingly, cluster analysis differentiates the consumption of fucosyl-oligosaccharides from galactosyl-oligosaccharides, highlighting the disappearance of Akkermansia genus in both fucosyl-oligosaccharides.
33938391	8	70	from	acetate	1583:1589	arg1	colon					1598:1602	the colon	1594:1602	the colon	1594:1602	The 3FN-feeding group showed increased levels of butyrate and acetate in the colon, and decreased levels of serum HDL-cholesterol.
33938391	8	71	theme	HDL-cholesterol	1635:1649	arg1	levels					1560:1565	increased levels	1550:1565	increased levels of butyrate and acetate in the colon	1550:1602	The 3FN-feeding group showed increased levels of butyrate and acetate in the colon, and decreased levels of serum HDL-cholesterol.
33938391	8	71	theme	HDL-cholesterol	1635:1649	arg1	levels					1619:1624	decreased levels	1609:1624	decreased levels of serum HDL-cholesterol	1609:1649	The 3FN-feeding group showed increased levels of butyrate and acetate in the colon, and decreased levels of serum HDL-cholesterol.
33938391	6	72	theme	absolute	1098:1105	arg1	levels					1107:1112	the absolute levels	1094:1112	the absolute levels	1094:1112	As well, LNB significantly increased the relative abundance of Bifidobacterium, whereas the absolute levels of this genus, as measured by quantitative real-time PCR, did not significantly increase.
33938391	4	73	from	disappearance	849:861	arg1	fucosyl-oligosaccharides					892:915	both fucosyl-oligosaccharides	887:915	both fucosyl-oligosaccharides	887:915	Interestingly, cluster analysis differentiates the consumption of fucosyl-oligosaccharides from galactosyl-oligosaccharides, highlighting the disappearance of Akkermansia genus in both fucosyl-oligosaccharides.
33938391	9	74	theme	cytokine	1697:1704	arg1	TNF-α					1706:1710	the pro-inflammatory cytokine TNF-α	1676:1710	the pro-inflammatory cytokine TNF-α	1676:1710	3FN also down-regulated the pro-inflammatory cytokine TNF-α and up-regulated the anti-inflammatory cytokines IL-10 and IL-13, and the Toll-like receptor 2 in the large intestine tissue.
33938391	4	75	theme	Akkermansia	866:876	arg1	genus					878:882	Akkermansia genus	866:882	Akkermansia genus	866:882	Interestingly, cluster analysis differentiates the consumption of fucosyl-oligosaccharides from galactosyl-oligosaccharides, highlighting the disappearance of Akkermansia genus in both fucosyl-oligosaccharides.
33938391	2	76	theme	fucosyl-α1,6-GlcNAc	382:400	arg1	impact					345:350	the impact	341:350	the impact of fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto-N-biose (LNB) and galacto-N-biose on the fecal microbiota and host-microbiota interactions	341:498	A humanized mouse model generated by infant fecal transplantation was utilized here to evaluate the impact of fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto-N-biose (LNB) and galacto-N-biose on the fecal microbiota and host-microbiota interactions.
33938391	0	77	theme	gut	7:9	arg1	modulation					22:31	Infant gut microbiota modulation	0:31	Infant gut microbiota modulation by human milk	0:45	Infant gut microbiota modulation by human milk disaccharides in humanized microbiome mice.
33938391	4	78	from	galactosyl-oligosaccharides	803:829	arg1	fucosyl-oligosaccharides					773:796	fucosyl-oligosaccharides	773:796	fucosyl-oligosaccharides from galactosyl-oligosaccharides	773:829	Interestingly, cluster analysis differentiates the consumption of fucosyl-oligosaccharides from galactosyl-oligosaccharides, highlighting the disappearance of Akkermansia genus in both fucosyl-oligosaccharides.
33938391	4	78	from	galactosyl-oligosaccharides	803:829	arg1	consumption					758:768	the consumption	754:768	the consumption of fucosyl-oligosaccharides from galactosyl-oligosaccharides	754:829	Interestingly, cluster analysis differentiates the consumption of fucosyl-oligosaccharides from galactosyl-oligosaccharides, highlighting the disappearance of Akkermansia genus in both fucosyl-oligosaccharides.
33938391	1	79	theme	milk	97:100	arg1	glycans					102:108	Human milk glycans	91:108	Human milk glycans	91:108	Human milk glycans present a unique diversity of structures that suggest different mechanisms by which they may affect the infant microbiome development.
33938391	7	80	theme	specific	1414:1421	arg1	groups					1436:1441	the donor and specific disaccharide groups	1400:1441	groups	1436:1441	OTUs corresponding to the species Bifidobacterium longum, Bifidobacterium adolescentis and Ruminococcus gnavus were not present in the control after the 3-week intervention, but were shared among the donor and specific disaccharide groups, indicating that their survival is dependent on disaccharide supplementation.
33938391	0	81	theme	human	36:40	arg1	milk					42:45	human milk	36:45	human milk	36:45	Infant gut microbiota modulation by human milk disaccharides in humanized microbiome mice.
33938391	2	82	theme	lacto-N-biose	403:415	arg1	impact					345:350	the impact	341:350	the impact of fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto-N-biose (LNB) and galacto-N-biose on the fecal microbiota and host-microbiota interactions	341:498	A humanized mouse model generated by infant fecal transplantation was utilized here to evaluate the impact of fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto-N-biose (LNB) and galacto-N-biose on the fecal microbiota and host-microbiota interactions.
33938391	2	83	theme	fecal	450:454	arg1	microbiota					456:465	the fecal microbiota and host-microbiota interactions	446:498	microbiota	456:465	A humanized mouse model generated by infant fecal transplantation was utilized here to evaluate the impact of fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto-N-biose (LNB) and galacto-N-biose on the fecal microbiota and host-microbiota interactions.
33938391	3	84	theme	rRNA	505:508	arg1	sequencing					519:528	16S rRNA amplicon sequencing	501:528	16S rRNA amplicon sequencing	501:528	16S rRNA amplicon sequencing showed that certain bacterial genera significantly increased (Ruminococcus and Oscillospira) or decreased (Eubacterium and Clostridium) in all disaccharide-supplemented groups.
33938391	6	85	dep	quantitative	1144:1155	arg1	real-time					1157:1165	real-time	1157:1165	real-time	1157:1165	As well, LNB significantly increased the relative abundance of Bifidobacterium, whereas the absolute levels of this genus, as measured by quantitative real-time PCR, did not significantly increase.
33938391	8	86	theme	increased	1550:1558	arg1	levels					1560:1565	increased levels	1550:1565	increased levels of butyrate and acetate in the colon	1550:1602	The 3FN-feeding group showed increased levels of butyrate and acetate in the colon, and decreased levels of serum HDL-cholesterol.
33938391	1	87	theme	microbiome	221:230	arg1	development					232:242	the infant microbiome development	210:242	the infant microbiome development	210:242	Human milk glycans present a unique diversity of structures that suggest different mechanisms by which they may affect the infant microbiome development.
32536391	3	0	theme	phosphate	542:550	arg1	composites					552:561	chitosan-based calcium phosphate composites	519:561	chitosan-based calcium phosphate composites	519:561	In this work, chitosan-based calcium phosphate composites were prepared and loaded with moxifloxacin hydrochloride.
32536391	5	1	theme	complete	864:871	arg1	release					878:884	complete drug release	864:884	complete drug release	864:884	Results showed that the composites provided complete drug release over three days, and the selected composite formulation induced differentiation and proliferation of osteoblasts, while reducing bacterial count, inflammation and intra-medullary fibrosis in bone tissue specimens of osteomyelitis-induced animal model.
32536391	6	2	theme	in	1170:1171	arg1	composite					1232:1240	the in situ prepared antibiotic-loaded calcium phosphate chitosan composite	1166:1240	the in situ prepared antibiotic-loaded calcium phosphate chitosan composite	1166:1240	Hence, we can conclude that the in situ prepared antibiotic-loaded calcium phosphate chitosan composite is promising in preventing post-operative osteomyelitis, and is worthy of clinical experimentation.
32536391	6	2	theme	in	1170:1171	arg1	promising					1245:1253	promising	1245:1253	promising	1245:1253	Hence, we can conclude that the in situ prepared antibiotic-loaded calcium phosphate chitosan composite is promising in preventing post-operative osteomyelitis, and is worthy of clinical experimentation.
32536391	2	3	theme	biodegradable	365:377	arg1	carrier					379:385	a biodegradable carrier	363:385	a biodegradable carrier at surgery sites	363:402	The use of locally applied antibiotics loaded on a biodegradable carrier at surgery sites is hypothesized to prevent post-operative osteomyelitis, while providing site-specific drug release.
32536391	6	4	theme	chitosan	1223:1230	arg1	composite					1232:1240	the in situ prepared antibiotic-loaded calcium phosphate chitosan composite	1166:1240	the in situ prepared antibiotic-loaded calcium phosphate chitosan composite	1166:1240	Hence, we can conclude that the in situ prepared antibiotic-loaded calcium phosphate chitosan composite is promising in preventing post-operative osteomyelitis, and is worthy of clinical experimentation.
32536391	6	4	theme	chitosan	1223:1230	arg1	promising					1245:1253	promising	1245:1253	promising	1245:1253	Hence, we can conclude that the in situ prepared antibiotic-loaded calcium phosphate chitosan composite is promising in preventing post-operative osteomyelitis, and is worthy of clinical experimentation.
32536391	5	5	theme	animal	1124:1129	arg1	model					1131:1135	osteomyelitis-induced animal model	1102:1135	osteomyelitis-induced animal model	1102:1135	Results showed that the composites provided complete drug release over three days, and the selected composite formulation induced differentiation and proliferation of osteoblasts, while reducing bacterial count, inflammation and intra-medullary fibrosis in bone tissue specimens of osteomyelitis-induced animal model.
32536391	0	6	dep	in	127:128	arg1	vivo					139:142	vivo	139:142	vivo	139:142	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
32536391	4	7	theme	phosphates	654:663	arg1	formation					633:641	The in-situ formation	621:641	The in-situ formation of calcium phosphates within the composite	621:684	The in-situ formation of calcium phosphates within the composite was experimentally confirmed by Fourier transform infra-red spectroscopy, X-ray powder diffraction, and scanning electron microscopy.
32536391	5	8	theme	tissue	1082:1087	arg1	specimens					1089:1097	bone tissue specimens	1077:1097	bone tissue specimens of osteomyelitis-induced animal model	1077:1135	Results showed that the composites provided complete drug release over three days, and the selected composite formulation induced differentiation and proliferation of osteoblasts, while reducing bacterial count, inflammation and intra-medullary fibrosis in bone tissue specimens of osteomyelitis-induced animal model.
32536391	3	9	theme	moxifloxacin	593:604	arg1	hydrochloride					606:618	moxifloxacin hydrochloride	593:618	moxifloxacin hydrochloride	593:618	In this work, chitosan-based calcium phosphate composites were prepared and loaded with moxifloxacin hydrochloride.
32536391	4	10	theme	electron	799:806	arg1	microscopy					808:817	scanning electron microscopy	790:817	scanning electron microscopy	790:817	The in-situ formation of calcium phosphates within the composite was experimentally confirmed by Fourier transform infra-red spectroscopy, X-ray powder diffraction, and scanning electron microscopy.
32536391	0	11	theme	in	127:128	arg1	evaluation					144:153	in vitro-in vivo evaluation	127:153	in vitro-in vivo evaluation	127:153	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
32536391	0	11	theme	in	127:128	arg1	scaffolds					37:45	Chitosan-calcium phosphate composite scaffolds	0:45	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.	0:154	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
32536391	4	12	theme	scanning	790:797	arg1	microscopy					808:817	scanning electron microscopy	790:817	scanning electron microscopy	790:817	The in-situ formation of calcium phosphates within the composite was experimentally confirmed by Fourier transform infra-red spectroscopy, X-ray powder diffraction, and scanning electron microscopy.
32536391	5	13	theme	composite	920:928	arg1	formulation					930:940	the selected composite formulation	907:940	the selected composite formulation	907:940	Results showed that the composites provided complete drug release over three days, and the selected composite formulation induced differentiation and proliferation of osteoblasts, while reducing bacterial count, inflammation and intra-medullary fibrosis in bone tissue specimens of osteomyelitis-induced animal model.
32536391	6	14	dep	in	1170:1171	arg1	situ					1173:1176	situ	1173:1176	situ	1173:1176	Hence, we can conclude that the in situ prepared antibiotic-loaded calcium phosphate chitosan composite is promising in preventing post-operative osteomyelitis, and is worthy of clinical experimentation.
32536391	1	15	theme	inflammatory	187:198	arg1	disease					200:206	a progressive inflammatory disease	173:206	a progressive inflammatory disease requiring prolonged systemic treatment with high antibiotic doses	173:272	Osteomyelitis is a progressive inflammatory disease requiring prolonged systemic treatment with high antibiotic doses, and is very challenging to be treated.
32536391	1	15	theme	inflammatory	187:198	arg1	Osteomyelitis					156:168	Osteomyelitis	156:168	Osteomyelitis	156:168	Osteomyelitis is a progressive inflammatory disease requiring prolonged systemic treatment with high antibiotic doses, and is very challenging to be treated.
32536391	4	16	theme	calcium	646:652	arg1	phosphates					654:663	calcium phosphates	646:663	calcium phosphates	646:663	The in-situ formation of calcium phosphates within the composite was experimentally confirmed by Fourier transform infra-red spectroscopy, X-ray powder diffraction, and scanning electron microscopy.
32536391	0	17	theme	Chitosan-calcium	0:15	arg1	evaluation					144:153	in vitro-in vivo evaluation	127:153	in vitro-in vivo evaluation	127:153	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
32536391	0	17	theme	Chitosan-calcium	0:15	arg1	Fabrication					92:102	Fabrication	92:102	Fabrication	92:102	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
32536391	0	17	theme	Chitosan-calcium	0:15	arg1	scaffolds					37:45	Chitosan-calcium phosphate composite scaffolds	0:45	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.	0:154	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
32536391	0	17	theme	Chitosan-calcium	0:15	arg1	characterization					105:120	characterization	105:120	characterization	105:120	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
32536391	6	18	theme	phosphate	1213:1221	arg1	composite					1232:1240	the in situ prepared antibiotic-loaded calcium phosphate chitosan composite	1166:1240	the in situ prepared antibiotic-loaded calcium phosphate chitosan composite	1166:1240	Hence, we can conclude that the in situ prepared antibiotic-loaded calcium phosphate chitosan composite is promising in preventing post-operative osteomyelitis, and is worthy of clinical experimentation.
32536391	6	18	theme	phosphate	1213:1221	arg1	promising					1245:1253	promising	1245:1253	promising	1245:1253	Hence, we can conclude that the in situ prepared antibiotic-loaded calcium phosphate chitosan composite is promising in preventing post-operative osteomyelitis, and is worthy of clinical experimentation.
32536391	6	19	theme	antibiotic-loaded	1187:1203	arg1	composite					1232:1240	the in situ prepared antibiotic-loaded calcium phosphate chitosan composite	1166:1240	the in situ prepared antibiotic-loaded calcium phosphate chitosan composite	1166:1240	Hence, we can conclude that the in situ prepared antibiotic-loaded calcium phosphate chitosan composite is promising in preventing post-operative osteomyelitis, and is worthy of clinical experimentation.
32536391	6	19	theme	antibiotic-loaded	1187:1203	arg1	promising					1245:1253	promising	1245:1253	promising	1245:1253	Hence, we can conclude that the in situ prepared antibiotic-loaded calcium phosphate chitosan composite is promising in preventing post-operative osteomyelitis, and is worthy of clinical experimentation.
32536391	2	20	theme	drug	491:494	arg1	release					496:502	site-specific drug release	477:502	site-specific drug release	477:502	The use of locally applied antibiotics loaded on a biodegradable carrier at surgery sites is hypothesized to prevent post-operative osteomyelitis, while providing site-specific drug release.
32536391	6	21	theme	calcium	1205:1211	arg1	composite					1232:1240	the in situ prepared antibiotic-loaded calcium phosphate chitosan composite	1166:1240	the in situ prepared antibiotic-loaded calcium phosphate chitosan composite	1166:1240	Hence, we can conclude that the in situ prepared antibiotic-loaded calcium phosphate chitosan composite is promising in preventing post-operative osteomyelitis, and is worthy of clinical experimentation.
32536391	6	21	theme	calcium	1205:1211	arg1	promising					1245:1253	promising	1245:1253	promising	1245:1253	Hence, we can conclude that the in situ prepared antibiotic-loaded calcium phosphate chitosan composite is promising in preventing post-operative osteomyelitis, and is worthy of clinical experimentation.
32536391	5	22	from	inflammation	1032:1043	arg1	specimens					1089:1097	bone tissue specimens	1077:1097	bone tissue specimens of osteomyelitis-induced animal model	1077:1135	Results showed that the composites provided complete drug release over three days, and the selected composite formulation induced differentiation and proliferation of osteoblasts, while reducing bacterial count, inflammation and intra-medullary fibrosis in bone tissue specimens of osteomyelitis-induced animal model.
32536391	2	23	theme	applied	333:339	arg1	antibiotics					341:351	locally applied antibiotics	325:351	locally applied antibiotics loaded on a biodegradable carrier at surgery sites	325:402	The use of locally applied antibiotics loaded on a biodegradable carrier at surgery sites is hypothesized to prevent post-operative osteomyelitis, while providing site-specific drug release.
32536391	6	24	theme	prepared	1178:1185	arg1	composite					1232:1240	the in situ prepared antibiotic-loaded calcium phosphate chitosan composite	1166:1240	the in situ prepared antibiotic-loaded calcium phosphate chitosan composite	1166:1240	Hence, we can conclude that the in situ prepared antibiotic-loaded calcium phosphate chitosan composite is promising in preventing post-operative osteomyelitis, and is worthy of clinical experimentation.
32536391	6	24	theme	prepared	1178:1185	arg1	promising					1245:1253	promising	1245:1253	promising	1245:1253	Hence, we can conclude that the in situ prepared antibiotic-loaded calcium phosphate chitosan composite is promising in preventing post-operative osteomyelitis, and is worthy of clinical experimentation.
32536391	2	25	theme	site-specific	477:489	arg1	release					496:502	site-specific drug release	477:502	site-specific drug release	477:502	The use of locally applied antibiotics loaded on a biodegradable carrier at surgery sites is hypothesized to prevent post-operative osteomyelitis, while providing site-specific drug release.
32536391	5	26	theme	intra-medullary	1049:1063	arg1	fibrosis					1065:1072	intra-medullary fibrosis	1049:1072	intra-medullary fibrosis	1049:1072	Results showed that the composites provided complete drug release over three days, and the selected composite formulation induced differentiation and proliferation of osteoblasts, while reducing bacterial count, inflammation and intra-medullary fibrosis in bone tissue specimens of osteomyelitis-induced animal model.
32536391	5	26	theme	intra-medullary	1049:1063	arg1	count					1025:1029	bacterial count	1015:1029	bacterial count	1015:1029	Results showed that the composites provided complete drug release over three days, and the selected composite formulation induced differentiation and proliferation of osteoblasts, while reducing bacterial count, inflammation and intra-medullary fibrosis in bone tissue specimens of osteomyelitis-induced animal model.
32536391	1	27	theme	prolonged	218:226	arg1	treatment					237:245	prolonged systemic treatment	218:245	prolonged systemic treatment with high antibiotic doses	218:272	Osteomyelitis is a progressive inflammatory disease requiring prolonged systemic treatment with high antibiotic doses, and is very challenging to be treated.
32536391	1	28	theme	systemic	228:235	arg1	treatment					237:245	prolonged systemic treatment	218:245	prolonged systemic treatment with high antibiotic doses	218:272	Osteomyelitis is a progressive inflammatory disease requiring prolonged systemic treatment with high antibiotic doses, and is very challenging to be treated.
32536391	0	29	theme	composite	27:35	arg1	evaluation					144:153	in vitro-in vivo evaluation	127:153	in vitro-in vivo evaluation	127:153	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
32536391	0	29	theme	composite	27:35	arg1	Fabrication					92:102	Fabrication	92:102	Fabrication	92:102	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
32536391	0	29	theme	composite	27:35	arg1	scaffolds					37:45	Chitosan-calcium phosphate composite scaffolds	0:45	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.	0:154	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
32536391	0	29	theme	composite	27:35	arg1	characterization					105:120	characterization	105:120	characterization	105:120	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
32536391	4	30	theme	in-situ	625:631	arg1	formation					633:641	The in-situ formation	621:641	The in-situ formation of calcium phosphates within the composite	621:684	The in-situ formation of calcium phosphates within the composite was experimentally confirmed by Fourier transform infra-red spectroscopy, X-ray powder diffraction, and scanning electron microscopy.
32536391	6	31	theme	experimentation	1325:1339	arg1	worthy					1306:1311	worthy	1306:1311	worthy of clinical experimentation	1306:1339	Hence, we can conclude that the in situ prepared antibiotic-loaded calcium phosphate chitosan composite is promising in preventing post-operative osteomyelitis, and is worthy of clinical experimentation.
32536391	1	32	theme	progressive	175:185	arg1	disease					200:206	a progressive inflammatory disease	173:206	a progressive inflammatory disease requiring prolonged systemic treatment with high antibiotic doses	173:272	Osteomyelitis is a progressive inflammatory disease requiring prolonged systemic treatment with high antibiotic doses, and is very challenging to be treated.
32536391	1	32	theme	progressive	175:185	arg1	Osteomyelitis					156:168	Osteomyelitis	156:168	Osteomyelitis	156:168	Osteomyelitis is a progressive inflammatory disease requiring prolonged systemic treatment with high antibiotic doses, and is very challenging to be treated.
32536391	4	33	theme	powder	766:771	arg1	diffraction					773:783	X-ray powder diffraction	760:783	X-ray powder diffraction	760:783	The in-situ formation of calcium phosphates within the composite was experimentally confirmed by Fourier transform infra-red spectroscopy, X-ray powder diffraction, and scanning electron microscopy.
32536391	5	34	theme	drug	873:876	arg1	release					878:884	complete drug release	864:884	complete drug release	864:884	Results showed that the composites provided complete drug release over three days, and the selected composite formulation induced differentiation and proliferation of osteoblasts, while reducing bacterial count, inflammation and intra-medullary fibrosis in bone tissue specimens of osteomyelitis-induced animal model.
32536391	4	35	dep	Fourier	718:724	arg1	transform					726:734	transform	726:734	transform infra-red spectroscopy, X-ray powder diffraction, and scanning electron microscopy	726:817	The in-situ formation of calcium phosphates within the composite was experimentally confirmed by Fourier transform infra-red spectroscopy, X-ray powder diffraction, and scanning electron microscopy.
32536391	2	36	theme	post-operative	431:444	arg1	osteomyelitis					446:458	post-operative osteomyelitis	431:458	post-operative osteomyelitis	431:458	The use of locally applied antibiotics loaded on a biodegradable carrier at surgery sites is hypothesized to prevent post-operative osteomyelitis, while providing site-specific drug release.
32536391	4	37	theme	infra-red	736:744	arg1	spectroscopy					746:757	infra-red spectroscopy	736:757	infra-red spectroscopy	736:757	The in-situ formation of calcium phosphates within the composite was experimentally confirmed by Fourier transform infra-red spectroscopy, X-ray powder diffraction, and scanning electron microscopy.
32536391	1	38	with	treatment	237:245	arg1	doses					268:272	high antibiotic doses	252:272	high antibiotic doses	252:272	Osteomyelitis is a progressive inflammatory disease requiring prolonged systemic treatment with high antibiotic doses, and is very challenging to be treated.
32536391	1	39	theme	high	252:255	arg1	doses					268:272	high antibiotic doses	252:272	high antibiotic doses	252:272	Osteomyelitis is a progressive inflammatory disease requiring prolonged systemic treatment with high antibiotic doses, and is very challenging to be treated.
32536391	0	40	theme	post-operative	62:75	arg1	osteomyelitis					77:89	post-operative osteomyelitis	62:89	post-operative osteomyelitis	62:89	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
32536391	5	41	theme	osteoblasts	987:997	arg1	proliferation					970:982	proliferation	970:982	proliferation	970:982	Results showed that the composites provided complete drug release over three days, and the selected composite formulation induced differentiation and proliferation of osteoblasts, while reducing bacterial count, inflammation and intra-medullary fibrosis in bone tissue specimens of osteomyelitis-induced animal model.
32536391	5	41	theme	osteoblasts	987:997	arg1	differentiation					950:964	differentiation	950:964	differentiation	950:964	Results showed that the composites provided complete drug release over three days, and the selected composite formulation induced differentiation and proliferation of osteoblasts, while reducing bacterial count, inflammation and intra-medullary fibrosis in bone tissue specimens of osteomyelitis-induced animal model.
32536391	2	42	from	sites	398:402	arg1	carrier					379:385	a biodegradable carrier	363:385	a biodegradable carrier at surgery sites	363:402	The use of locally applied antibiotics loaded on a biodegradable carrier at surgery sites is hypothesized to prevent post-operative osteomyelitis, while providing site-specific drug release.
32536391	3	43	theme	chitosan-based	519:532	arg1	composites					552:561	chitosan-based calcium phosphate composites	519:561	chitosan-based calcium phosphate composites	519:561	In this work, chitosan-based calcium phosphate composites were prepared and loaded with moxifloxacin hydrochloride.
32536391	6	44	theme	clinical	1316:1323	arg1	experimentation					1325:1339	clinical experimentation	1316:1339	clinical experimentation	1316:1339	Hence, we can conclude that the in situ prepared antibiotic-loaded calcium phosphate chitosan composite is promising in preventing post-operative osteomyelitis, and is worthy of clinical experimentation.
32536391	3	45	theme	calcium	534:540	arg1	composites					552:561	chitosan-based calcium phosphate composites	519:561	chitosan-based calcium phosphate composites	519:561	In this work, chitosan-based calcium phosphate composites were prepared and loaded with moxifloxacin hydrochloride.
32536391	5	46	from	fibrosis	1065:1072	arg1	specimens					1089:1097	bone tissue specimens	1077:1097	bone tissue specimens of osteomyelitis-induced animal model	1077:1135	Results showed that the composites provided complete drug release over three days, and the selected composite formulation induced differentiation and proliferation of osteoblasts, while reducing bacterial count, inflammation and intra-medullary fibrosis in bone tissue specimens of osteomyelitis-induced animal model.
32536391	5	47	theme	osteomyelitis-induced	1102:1122	arg1	model					1131:1135	osteomyelitis-induced animal model	1102:1135	osteomyelitis-induced animal model	1102:1135	Results showed that the composites provided complete drug release over three days, and the selected composite formulation induced differentiation and proliferation of osteoblasts, while reducing bacterial count, inflammation and intra-medullary fibrosis in bone tissue specimens of osteomyelitis-induced animal model.
32536391	0	48	dep	scaffolds	37:45	arg1	evaluation					144:153	in vitro-in vivo evaluation	127:153	in vitro-in vivo evaluation	127:153	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
32536391	0	48	dep	scaffolds	37:45	arg1	Fabrication					92:102	Fabrication	92:102	Fabrication	92:102	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
32536391	0	48	dep	scaffolds	37:45	arg1	scaffolds					37:45	Chitosan-calcium phosphate composite scaffolds	0:45	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.	0:154	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
32536391	0	48	dep	scaffolds	37:45	arg1	characterization					105:120	characterization	105:120	characterization	105:120	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
32536391	4	49	theme	X-ray	760:764	arg1	diffraction					773:783	X-ray powder diffraction	760:783	X-ray powder diffraction	760:783	The in-situ formation of calcium phosphates within the composite was experimentally confirmed by Fourier transform infra-red spectroscopy, X-ray powder diffraction, and scanning electron microscopy.
32536391	1	50	theme	antibiotic	257:266	arg1	doses					268:272	high antibiotic doses	252:272	high antibiotic doses	252:272	Osteomyelitis is a progressive inflammatory disease requiring prolonged systemic treatment with high antibiotic doses, and is very challenging to be treated.
32536391	5	51	theme	bacterial	1015:1023	arg1	fibrosis					1065:1072	intra-medullary fibrosis	1049:1072	intra-medullary fibrosis	1049:1072	Results showed that the composites provided complete drug release over three days, and the selected composite formulation induced differentiation and proliferation of osteoblasts, while reducing bacterial count, inflammation and intra-medullary fibrosis in bone tissue specimens of osteomyelitis-induced animal model.
32536391	5	51	theme	bacterial	1015:1023	arg1	count					1025:1029	bacterial count	1015:1029	bacterial count	1015:1029	Results showed that the composites provided complete drug release over three days, and the selected composite formulation induced differentiation and proliferation of osteoblasts, while reducing bacterial count, inflammation and intra-medullary fibrosis in bone tissue specimens of osteomyelitis-induced animal model.
32536391	5	51	theme	bacterial	1015:1023	arg1	inflammation					1032:1043	inflammation	1032:1043	inflammation	1032:1043	Results showed that the composites provided complete drug release over three days, and the selected composite formulation induced differentiation and proliferation of osteoblasts, while reducing bacterial count, inflammation and intra-medullary fibrosis in bone tissue specimens of osteomyelitis-induced animal model.
32536391	5	52	theme	bone	1077:1080	arg1	specimens					1089:1097	bone tissue specimens	1077:1097	bone tissue specimens of osteomyelitis-induced animal model	1077:1135	Results showed that the composites provided complete drug release over three days, and the selected composite formulation induced differentiation and proliferation of osteoblasts, while reducing bacterial count, inflammation and intra-medullary fibrosis in bone tissue specimens of osteomyelitis-induced animal model.
32536391	2	53	theme	antibiotics	341:351	arg1	use					318:320	The use	314:320	The use of locally applied antibiotics loaded on a biodegradable carrier at surgery sites	314:402	The use of locally applied antibiotics loaded on a biodegradable carrier at surgery sites is hypothesized to prevent post-operative osteomyelitis, while providing site-specific drug release.
32536391	6	54	theme	post-operative	1269:1282	arg1	osteomyelitis					1284:1296	post-operative osteomyelitis	1269:1296	post-operative osteomyelitis	1269:1296	Hence, we can conclude that the in situ prepared antibiotic-loaded calcium phosphate chitosan composite is promising in preventing post-operative osteomyelitis, and is worthy of clinical experimentation.
32536391	5	55	theme	model	1131:1135	arg1	specimens					1089:1097	bone tissue specimens	1077:1097	bone tissue specimens of osteomyelitis-induced animal model	1077:1135	Results showed that the composites provided complete drug release over three days, and the selected composite formulation induced differentiation and proliferation of osteoblasts, while reducing bacterial count, inflammation and intra-medullary fibrosis in bone tissue specimens of osteomyelitis-induced animal model.
32536391	5	56	theme	selected	911:918	arg1	formulation					930:940	the selected composite formulation	907:940	the selected composite formulation	907:940	Results showed that the composites provided complete drug release over three days, and the selected composite formulation induced differentiation and proliferation of osteoblasts, while reducing bacterial count, inflammation and intra-medullary fibrosis in bone tissue specimens of osteomyelitis-induced animal model.
32536391	2	57	theme	surgery	390:396	arg1	sites					398:402	surgery sites	390:402	surgery sites	390:402	The use of locally applied antibiotics loaded on a biodegradable carrier at surgery sites is hypothesized to prevent post-operative osteomyelitis, while providing site-specific drug release.
32536391	0	58	theme	osteomyelitis	77:89	arg1	control					51:57	control	51:57	control of post-operative osteomyelitis	51:89	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
32536391	0	59	theme	phosphate	17:25	arg1	evaluation					144:153	in vitro-in vivo evaluation	127:153	in vitro-in vivo evaluation	127:153	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
32536391	0	59	theme	phosphate	17:25	arg1	Fabrication					92:102	Fabrication	92:102	Fabrication	92:102	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
32536391	0	59	theme	phosphate	17:25	arg1	scaffolds					37:45	Chitosan-calcium phosphate composite scaffolds	0:45	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.	0:154	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
32536391	0	59	theme	phosphate	17:25	arg1	characterization					105:120	characterization	105:120	characterization	105:120	Chitosan-calcium phosphate composite scaffolds for control of post-operative osteomyelitis: Fabrication, characterization, and in vitro-in vivo evaluation.
33977982	7	0	theme	amylose	1293:1299	arg1	chains					1307:1312	amylose short chains	1293:1312	amylose short chains (degree of polymerization (DP) < 500)	1293:1350	On the other hand, the slowly digestible starch fraction was largely formed by intramolecular interactions among amylose short chains (degree of polymerization (DP) < 500).
33977982	4	1	theme	starch	762:767	arg1	fractions					780:788	two starch digestible fractions	758:788	two starch digestible fractions with distinct rate constants	758:817	By fitting starch digestograms to the logarithm of slop (LOS) plot and the combination of parallel and sequential kinetics model (CPS), two starch digestible fractions with distinct rate constants were identified.
33977982	2	2	theme	characteristics	374:388	arg1	alterations					279:289	The alterations	275:289	The alterations of chemical compositions, starch chain-length distributions (CLDs) and rheological characteristics of quinoa flours after the germination treatment	275:437	The alterations of chemical compositions, starch chain-length distributions (CLDs) and rheological characteristics of quinoa flours after the germination treatment were characterized.
33977982	1	3	theme	cooked	260:265	arg1	quinoa					267:272	cooked quinoa	260:272	cooked quinoa	260:272	A germination treatment was explored in this study as a green strategy to reduce the in vitro starch digestibility of cooked quinoa.
33977982	0	4	theme	gel	93:95	arg1	viscoelasticity					97:111	gel viscoelasticity	93:111	gel viscoelasticity	93:111	Chemical components and chain-length distributions affecting quinoa starch digestibility and gel viscoelasticity after germination treatment.
33977982	7	5	theme	other	1187:1191	arg1	hand					1193:1196	the other hand	1183:1196	the other hand	1183:1196	On the other hand, the slowly digestible starch fraction was largely formed by intramolecular interactions among amylose short chains (degree of polymerization (DP) < 500).
33977982	2	6	theme	rheological	362:372	arg1	characteristics					374:388	rheological characteristics	362:388	rheological characteristics of quinoa flours after the germination treatment	362:437	The alterations of chemical compositions, starch chain-length distributions (CLDs) and rheological characteristics of quinoa flours after the germination treatment were characterized.
33977982	1	7	theme	quinoa	267:272	arg1	digestibility					243:255	the in vitro starch digestibility	223:255	the in vitro starch digestibility of cooked quinoa	223:272	A germination treatment was explored in this study as a green strategy to reduce the in vitro starch digestibility of cooked quinoa.
33977982	0	8	theme	germination	119:129	arg1	treatment					131:139	germination treatment	119:139	germination treatment	119:139	Chemical components and chain-length distributions affecting quinoa starch digestibility and gel viscoelasticity after germination treatment.
33977982	4	9	theme	rate	804:807	arg1	constants					809:817	distinct rate constants	795:817	distinct rate constants	795:817	By fitting starch digestograms to the logarithm of slop (LOS) plot and the combination of parallel and sequential kinetics model (CPS), two starch digestible fractions with distinct rate constants were identified.
33977982	2	10	theme	compositions	303:314	arg1	alterations					279:289	The alterations	275:289	The alterations of chemical compositions, starch chain-length distributions (CLDs) and rheological characteristics of quinoa flours after the germination treatment	275:437	The alterations of chemical compositions, starch chain-length distributions (CLDs) and rheological characteristics of quinoa flours after the germination treatment were characterized.
33977982	8	11	theme	cereal	1426:1431	arg1	products					1433:1440	cereal products	1426:1440	cereal products with slower starch digestibility	1426:1473	These results suggest that germination may be a promising way to develop cereal products with slower starch digestibility.
33977982	5	12	theme	amylose	979:985	arg1	CLDs					987:990	amylose CLDs	979:990	amylose CLDs	979:990	Pearson correlation analysis further found that the observed starch digestive characteristics could be largely explained by the alterations of amylose CLDs caused by the germination treatment.
33977982	4	13	theme	digestible	769:778	arg1	fractions					780:788	two starch digestible fractions	758:788	two starch digestible fractions with distinct rate constants	758:817	By fitting starch digestograms to the logarithm of slop (LOS) plot and the combination of parallel and sequential kinetics model (CPS), two starch digestible fractions with distinct rate constants were identified.
33977982	2	14	theme	chemical	294:301	arg1	compositions					303:314	chemical compositions	294:314	chemical compositions	294:314	The alterations of chemical compositions, starch chain-length distributions (CLDs) and rheological characteristics of quinoa flours after the germination treatment were characterized.
33977982	4	15	theme	starch	633:638	arg1	digestograms					640:651	starch digestograms	633:651	starch digestograms to the logarithm of slop (LOS) plot	633:687	By fitting starch digestograms to the logarithm of slop (LOS) plot and the combination of parallel and sequential kinetics model (CPS), two starch digestible fractions with distinct rate constants were identified.
33977982	4	16	theme	slop	673:676	arg1	plot					684:687	slop (LOS) plot	673:687	slop (LOS) plot	673:687	By fitting starch digestograms to the logarithm of slop (LOS) plot and the combination of parallel and sequential kinetics model (CPS), two starch digestible fractions with distinct rate constants were identified.
33977982	3	17	theme	cooked	566:571	arg1	flours					580:585	cooked quinoa flours	566:585	cooked quinoa flours	566:585	Results showed that a significant alteration of amylose CLDs and the starch digestibility was observed for cooked quinoa flours after different germination times.
33977982	5	18	theme	digestive	904:912	arg1	characteristics					914:928	the observed starch digestive characteristics	884:928	the observed starch digestive characteristics	884:928	Pearson correlation analysis further found that the observed starch digestive characteristics could be largely explained by the alterations of amylose CLDs caused by the germination treatment.
33977982	2	19	theme	germination	417:427	arg1	treatment					429:437	the germination treatment	413:437	the germination treatment	413:437	The alterations of chemical compositions, starch chain-length distributions (CLDs) and rheological characteristics of quinoa flours after the germination treatment were characterized.
33977982	7	20	theme	intramolecular	1259:1272	arg1	interactions					1274:1285	intramolecular interactions	1259:1285	intramolecular interactions among amylose short chains (degree of polymerization (DP) < 500)	1259:1350	On the other hand, the slowly digestible starch fraction was largely formed by intramolecular interactions among amylose short chains (degree of polymerization (DP) < 500).
33977982	8	21	with	products	1433:1440	arg1	digestibility					1461:1473	slower starch digestibility	1447:1473	slower starch digestibility	1447:1473	These results suggest that germination may be a promising way to develop cereal products with slower starch digestibility.
33977982	4	22	theme	sequential	725:734	arg1	model					745:749	sequential kinetics model	725:749	sequential kinetics model	725:749	By fitting starch digestograms to the logarithm of slop (LOS) plot and the combination of parallel and sequential kinetics model (CPS), two starch digestible fractions with distinct rate constants were identified.
33977982	3	23	theme	quinoa	573:578	arg1	flours					580:585	cooked quinoa flours	566:585	cooked quinoa flours	566:585	Results showed that a significant alteration of amylose CLDs and the starch digestibility was observed for cooked quinoa flours after different germination times.
33977982	6	24	theme	intermolecular	1151:1164	arg1	crystallites					1166:1177	amylose intermolecular crystallites	1143:1177	amylose intermolecular crystallites	1143:1177	More specifically, the rapidly digestible starch fraction mainly consisted of amorphous amylopectin molecules and amylose intermolecular crystallites.
33977982	4	25	theme	plot	684:687	arg1	logarithm					660:668	the logarithm	656:668	the logarithm of slop (LOS) plot	656:687	By fitting starch digestograms to the logarithm of slop (LOS) plot and the combination of parallel and sequential kinetics model (CPS), two starch digestible fractions with distinct rate constants were identified.
33977982	7	26	theme	short	1301:1305	arg1	chains					1307:1312	amylose short chains	1293:1312	amylose short chains (degree of polymerization (DP) < 500)	1293:1350	On the other hand, the slowly digestible starch fraction was largely formed by intramolecular interactions among amylose short chains (degree of polymerization (DP) < 500).
33977982	6	27	theme	amylose	1143:1149	arg1	crystallites					1166:1177	amylose intermolecular crystallites	1143:1177	amylose intermolecular crystallites	1143:1177	More specifically, the rapidly digestible starch fraction mainly consisted of amorphous amylopectin molecules and amylose intermolecular crystallites.
33977982	2	28	theme	distributions	337:349	arg1	alterations					279:289	The alterations	275:289	The alterations of chemical compositions, starch chain-length distributions (CLDs) and rheological characteristics of quinoa flours after the germination treatment	275:437	The alterations of chemical compositions, starch chain-length distributions (CLDs) and rheological characteristics of quinoa flours after the germination treatment were characterized.
33977982	1	29	theme	green	198:202	arg1	treatment					156:164	A germination treatment	142:164	A germination treatment	142:164	A germination treatment was explored in this study as a green strategy to reduce the in vitro starch digestibility of cooked quinoa.
33977982	1	29	theme	green	198:202	arg1	strategy					204:211	a green strategy	196:211	a green strategy to reduce the in vitro starch digestibility of cooked quinoa	196:272	A germination treatment was explored in this study as a green strategy to reduce the in vitro starch digestibility of cooked quinoa.
33977982	0	30	theme	Chemical	0:7	arg1	components					9:18	Chemical components	0:18	Chemical components	0:18	Chemical components and chain-length distributions affecting quinoa starch digestibility and gel viscoelasticity after germination treatment.
33977982	5	31	theme	observed	888:895	arg1	characteristics					914:928	the observed starch digestive characteristics	884:928	the observed starch digestive characteristics	884:928	Pearson correlation analysis further found that the observed starch digestive characteristics could be largely explained by the alterations of amylose CLDs caused by the germination treatment.
33977982	2	32	theme	chain-length	324:335	arg1	CLDs					352:355	CLDs	352:355	CLDs	352:355	The alterations of chemical compositions, starch chain-length distributions (CLDs) and rheological characteristics of quinoa flours after the germination treatment were characterized.
33977982	2	32	theme	chain-length	324:335	arg1	distributions					337:349	starch chain-length distributions	317:349	starch chain-length distributions (CLDs)	317:356	The alterations of chemical compositions, starch chain-length distributions (CLDs) and rheological characteristics of quinoa flours after the germination treatment were characterized.
33977982	2	33	theme	flours	400:405	arg1	characteristics					374:388	rheological characteristics	362:388	rheological characteristics of quinoa flours after the germination treatment	362:437	The alterations of chemical compositions, starch chain-length distributions (CLDs) and rheological characteristics of quinoa flours after the germination treatment were characterized.
33977982	2	33	theme	flours	400:405	arg1	compositions					303:314	chemical compositions	294:314	chemical compositions	294:314	The alterations of chemical compositions, starch chain-length distributions (CLDs) and rheological characteristics of quinoa flours after the germination treatment were characterized.
33977982	2	33	theme	flours	400:405	arg1	CLDs					352:355	CLDs	352:355	CLDs	352:355	The alterations of chemical compositions, starch chain-length distributions (CLDs) and rheological characteristics of quinoa flours after the germination treatment were characterized.
33977982	2	33	theme	flours	400:405	arg1	distributions					337:349	starch chain-length distributions	317:349	starch chain-length distributions (CLDs)	317:356	The alterations of chemical compositions, starch chain-length distributions (CLDs) and rheological characteristics of quinoa flours after the germination treatment were characterized.
33977982	0	34	theme	chain-length	24:35	arg1	distributions					37:49	chain-length distributions	24:49	chain-length distributions	24:49	Chemical components and chain-length distributions affecting quinoa starch digestibility and gel viscoelasticity after germination treatment.
33977982	5	35	theme	Pearson	836:842	arg1	correlation					844:854	Pearson correlation	836:854	Pearson correlation analysis	836:863	Pearson correlation analysis further found that the observed starch digestive characteristics could be largely explained by the alterations of amylose CLDs caused by the germination treatment.
33977982	2	36	theme	starch	317:322	arg1	CLDs					352:355	CLDs	352:355	CLDs	352:355	The alterations of chemical compositions, starch chain-length distributions (CLDs) and rheological characteristics of quinoa flours after the germination treatment were characterized.
33977982	2	36	theme	starch	317:322	arg1	distributions					337:349	starch chain-length distributions	317:349	starch chain-length distributions (CLDs)	317:356	The alterations of chemical compositions, starch chain-length distributions (CLDs) and rheological characteristics of quinoa flours after the germination treatment were characterized.
33977982	6	37	theme	amorphous	1107:1115	arg1	molecules					1129:1137	amorphous amylopectin molecules	1107:1137	amorphous amylopectin molecules	1107:1137	More specifically, the rapidly digestible starch fraction mainly consisted of amorphous amylopectin molecules and amylose intermolecular crystallites.
33977982	5	38	theme	CLDs	987:990	arg1	alterations					964:974	the alterations	960:974	the alterations of amylose CLDs caused by the germination treatment	960:1026	Pearson correlation analysis further found that the observed starch digestive characteristics could be largely explained by the alterations of amylose CLDs caused by the germination treatment.
33977982	4	39	with	fractions	780:788	arg1	constants					809:817	distinct rate constants	795:817	distinct rate constants	795:817	By fitting starch digestograms to the logarithm of slop (LOS) plot and the combination of parallel and sequential kinetics model (CPS), two starch digestible fractions with distinct rate constants were identified.
33977982	4	40	theme	distinct	795:802	arg1	constants					809:817	distinct rate constants	795:817	distinct rate constants	795:817	By fitting starch digestograms to the logarithm of slop (LOS) plot and the combination of parallel and sequential kinetics model (CPS), two starch digestible fractions with distinct rate constants were identified.
33977982	3	41	theme	germination	603:613	arg1	times					615:619	different germination times	593:619	different germination times	593:619	Results showed that a significant alteration of amylose CLDs and the starch digestibility was observed for cooked quinoa flours after different germination times.
33977982	3	42	theme	amylose	507:513	arg1	CLDs					515:518	amylose CLDs	507:518	amylose CLDs	507:518	Results showed that a significant alteration of amylose CLDs and the starch digestibility was observed for cooked quinoa flours after different germination times.
33977982	7	43	theme	digestible	1210:1219	arg1	starch					1221:1226	the slowly digestible starch	1199:1226	the slowly digestible starch fraction	1199:1235	On the other hand, the slowly digestible starch fraction was largely formed by intramolecular interactions among amylose short chains (degree of polymerization (DP) < 500).
33977982	3	44	theme	starch	528:533	arg1	digestibility					535:547	the starch digestibility	524:547	the starch digestibility	524:547	Results showed that a significant alteration of amylose CLDs and the starch digestibility was observed for cooked quinoa flours after different germination times.
33977982	7	45	dep	chains	1307:1312	arg1	degree					1315:1320	degree	1315:1320	degree of polymerization (DP) < 500	1315:1349	On the other hand, the slowly digestible starch fraction was largely formed by intramolecular interactions among amylose short chains (degree of polymerization (DP) < 500).
33977982	7	46	theme	polymerization	1325:1338	arg1	degree					1315:1320	degree	1315:1320	degree of polymerization (DP) < 500	1315:1349	On the other hand, the slowly digestible starch fraction was largely formed by intramolecular interactions among amylose short chains (degree of polymerization (DP) < 500).
33977982	8	47	theme	slower	1447:1452	arg1	digestibility					1461:1473	slower starch digestibility	1447:1473	slower starch digestibility	1447:1473	These results suggest that germination may be a promising way to develop cereal products with slower starch digestibility.
33977982	6	48	theme	amylopectin	1117:1127	arg1	molecules					1129:1137	amorphous amylopectin molecules	1107:1137	amorphous amylopectin molecules	1107:1137	More specifically, the rapidly digestible starch fraction mainly consisted of amorphous amylopectin molecules and amylose intermolecular crystallites.
33977982	3	49	theme	significant	481:491	arg1	alteration					493:502	a significant alteration	479:502	a significant alteration of amylose CLDs	479:518	Results showed that a significant alteration of amylose CLDs and the starch digestibility was observed for cooked quinoa flours after different germination times.
33977982	4	50	theme	parallel	712:719	arg1	CPS					752:754	CPS	752:754	CPS	752:754	By fitting starch digestograms to the logarithm of slop (LOS) plot and the combination of parallel and sequential kinetics model (CPS), two starch digestible fractions with distinct rate constants were identified.
33977982	4	50	theme	parallel	712:719	arg1	model					745:749	sequential kinetics model	725:749	sequential kinetics model	725:749	By fitting starch digestograms to the logarithm of slop (LOS) plot and the combination of parallel and sequential kinetics model (CPS), two starch digestible fractions with distinct rate constants were identified.
33977982	4	50	theme	parallel	712:719	arg1	digestograms					640:651	starch digestograms	633:651	starch digestograms to the logarithm of slop (LOS) plot	633:687	By fitting starch digestograms to the logarithm of slop (LOS) plot and the combination of parallel and sequential kinetics model (CPS), two starch digestible fractions with distinct rate constants were identified.
33977982	4	50	theme	parallel	712:719	arg1	combination					697:707	the combination	693:707	the combination of parallel	693:719	By fitting starch digestograms to the logarithm of slop (LOS) plot and the combination of parallel and sequential kinetics model (CPS), two starch digestible fractions with distinct rate constants were identified.
33977982	7	51	theme	starch	1221:1226	arg1	fraction					1228:1235	the slowly digestible starch fraction	1199:1235	the slowly digestible starch fraction	1199:1235	On the other hand, the slowly digestible starch fraction was largely formed by intramolecular interactions among amylose short chains (degree of polymerization (DP) < 500).
33977982	5	52	theme	correlation	844:854	arg1	analysis					856:863	Pearson correlation analysis	836:863	Pearson correlation analysis	836:863	Pearson correlation analysis further found that the observed starch digestive characteristics could be largely explained by the alterations of amylose CLDs caused by the germination treatment.
33977982	1	53	theme	in	227:228	arg1	digestibility					243:255	the in vitro starch digestibility	223:255	the in vitro starch digestibility of cooked quinoa	223:272	A germination treatment was explored in this study as a green strategy to reduce the in vitro starch digestibility of cooked quinoa.
33977982	0	54	theme	starch	68:73	arg1	digestibility					75:87	quinoa starch digestibility	61:87	quinoa starch digestibility	61:87	Chemical components and chain-length distributions affecting quinoa starch digestibility and gel viscoelasticity after germination treatment.
33977982	5	55	theme	germination	1006:1016	arg1	treatment					1018:1026	the germination treatment	1002:1026	the germination treatment	1002:1026	Pearson correlation analysis further found that the observed starch digestive characteristics could be largely explained by the alterations of amylose CLDs caused by the germination treatment.
33977982	1	56	theme	A	142:142	arg1	treatment					156:164	A germination treatment	142:164	A germination treatment	142:164	A germination treatment was explored in this study as a green strategy to reduce the in vitro starch digestibility of cooked quinoa.
33977982	1	56	theme	A	142:142	arg1	strategy					204:211	a green strategy	196:211	a green strategy to reduce the in vitro starch digestibility of cooked quinoa	196:272	A germination treatment was explored in this study as a green strategy to reduce the in vitro starch digestibility of cooked quinoa.
33977982	0	57	theme	quinoa	61:66	arg1	digestibility					75:87	quinoa starch digestibility	61:87	quinoa starch digestibility	61:87	Chemical components and chain-length distributions affecting quinoa starch digestibility and gel viscoelasticity after germination treatment.
33977982	8	58	theme	starch	1454:1459	arg1	digestibility					1461:1473	slower starch digestibility	1447:1473	slower starch digestibility	1447:1473	These results suggest that germination may be a promising way to develop cereal products with slower starch digestibility.
33977982	1	59	theme	germination	144:154	arg1	treatment					156:164	A germination treatment	142:164	A germination treatment	142:164	A germination treatment was explored in this study as a green strategy to reduce the in vitro starch digestibility of cooked quinoa.
33977982	1	59	theme	germination	144:154	arg1	strategy					204:211	a green strategy	196:211	a green strategy to reduce the in vitro starch digestibility of cooked quinoa	196:272	A germination treatment was explored in this study as a green strategy to reduce the in vitro starch digestibility of cooked quinoa.
33977982	6	60	theme	starch	1071:1076	arg1	fraction					1078:1085	the rapidly digestible starch fraction	1048:1085	the rapidly digestible starch fraction	1048:1085	More specifically, the rapidly digestible starch fraction mainly consisted of amorphous amylopectin molecules and amylose intermolecular crystallites.
33977982	3	61	theme	CLDs	515:518	arg1	alteration					493:502	a significant alteration	479:502	a significant alteration of amylose CLDs	479:518	Results showed that a significant alteration of amylose CLDs and the starch digestibility was observed for cooked quinoa flours after different germination times.
33977982	3	61	theme	CLDs	515:518	arg1	digestibility					535:547	the starch digestibility	524:547	the starch digestibility	524:547	Results showed that a significant alteration of amylose CLDs and the starch digestibility was observed for cooked quinoa flours after different germination times.
33977982	3	62	theme	different	593:601	arg1	times					615:619	different germination times	593:619	different germination times	593:619	Results showed that a significant alteration of amylose CLDs and the starch digestibility was observed for cooked quinoa flours after different germination times.
33977982	8	63	theme	promising	1401:1409	arg1	way					1411:1413	a promising way	1399:1413	a promising way to develop cereal products with slower starch digestibility	1399:1473	These results suggest that germination may be a promising way to develop cereal products with slower starch digestibility.
33977982	8	63	theme	promising	1401:1409	arg1	germination					1380:1390	germination	1380:1390	germination	1380:1390	These results suggest that germination may be a promising way to develop cereal products with slower starch digestibility.
33977982	1	64	dep	in	227:228	arg1	vitro					230:234	vitro	230:234	vitro	230:234	A germination treatment was explored in this study as a green strategy to reduce the in vitro starch digestibility of cooked quinoa.
33977982	1	65	theme	starch	236:241	arg1	digestibility					243:255	the in vitro starch digestibility	223:255	the in vitro starch digestibility of cooked quinoa	223:272	A germination treatment was explored in this study as a green strategy to reduce the in vitro starch digestibility of cooked quinoa.
33977982	4	66	theme	LOS	679:681	arg1	plot					684:687	slop (LOS) plot	673:687	slop (LOS) plot	673:687	By fitting starch digestograms to the logarithm of slop (LOS) plot and the combination of parallel and sequential kinetics model (CPS), two starch digestible fractions with distinct rate constants were identified.
33977982	2	67	theme	quinoa	393:398	arg1	flours					400:405	quinoa flours	393:405	quinoa flours after the germination treatment	393:437	The alterations of chemical compositions, starch chain-length distributions (CLDs) and rheological characteristics of quinoa flours after the germination treatment were characterized.
33977982	5	68	theme	starch	897:902	arg1	characteristics					914:928	the observed starch digestive characteristics	884:928	the observed starch digestive characteristics	884:928	Pearson correlation analysis further found that the observed starch digestive characteristics could be largely explained by the alterations of amylose CLDs caused by the germination treatment.
33977982	6	69	theme	digestible	1060:1069	arg1	starch					1071:1076	the rapidly digestible starch	1048:1076	the rapidly digestible starch fraction	1048:1085	More specifically, the rapidly digestible starch fraction mainly consisted of amorphous amylopectin molecules and amylose intermolecular crystallites.
33977982	4	70	theme	kinetics	736:743	arg1	model					745:749	sequential kinetics model	725:749	sequential kinetics model	725:749	By fitting starch digestograms to the logarithm of slop (LOS) plot and the combination of parallel and sequential kinetics model (CPS), two starch digestible fractions with distinct rate constants were identified.
34010697	12	0	theme	SDS-polysaccharide	1773:1790	arg1	defensin					1736:1743	the defensin	1732:1743	Inflammation Drosophila especially the defensin (4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group: SDS group, p < 0.001)	1697:1819	In addition, pPMTLs significantly improved expression level of immune-related genes in Inflammation Drosophila especially the defensin (4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group: SDS group, p < 0.001).
34010697	12	0	theme	SDS-polysaccharide	1773:1790	arg1	group					1792:1796	4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group	1746:1796	4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group	1746:1796	In addition, pPMTLs significantly improved expression level of immune-related genes in Inflammation Drosophila especially the defensin (4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group: SDS group, p < 0.001).
34010697	6	1	theme	polysaccharide	805:818	arg1	extract					829:835	Premna microphylla turcz polysaccharide (pPMTLs) extract	780:835	Premna microphylla turcz polysaccharide (pPMTLs) extract	780:835	The treatment group contained Premna microphylla turcz polysaccharide (pPMTLs) extract.
34010697	8	2	theme	electron	1135:1142	arg1	microscope					1144:1153	transmission electron microscope	1122:1153	transmission electron microscope	1122:1153	The microvilli and nucleus of the midgut epithelial cells of different treatments were observed by transmission electron microscope, and the expression of inflammation-related genes was detected by real-time quantitative PCR (qRT-PCR).
34010697	1	3	used	used	197:200	arg2	remedy					212:217	a folk remedy	205:217	a folk remedy	205:217	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz is traditionally used as a folk remedy.
34010697	1	3	used	used	197:200	arg2	turcz					174:178	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz	124:178	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz	124:178	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz is traditionally used as a folk remedy.
34010697	4	4	from	reports	479:485	arg1	effects					532:538	the anti-inflammatory effects	510:538	the anti-inflammatory effects of polysaccharide, which was the main component in Premna microphylla turcz	510:614	However, there are few reports of in-depth studies on the anti-inflammatory effects of polysaccharide, which was the main component in Premna microphylla turcz.
34010697	6	5	theme	microphylla	787:797	arg1	extract					829:835	Premna microphylla turcz polysaccharide (pPMTLs) extract	780:835	Premna microphylla turcz polysaccharide (pPMTLs) extract	780:835	The treatment group contained Premna microphylla turcz polysaccharide (pPMTLs) extract.
34010697	14	6	dep	CONCLUSIONS	1964:1974	arg1	revealed					2002:2009	revealed	2002:2009	revealed the potential application of pPMTLs in enhancing inflammation defense, which would be enormous significance for the inflammation-related disorders treatment	2002:2166	CONCLUSIONS Collectively, our results revealed the potential application of pPMTLs in enhancing inflammation defense, which would be enormous significance for the inflammation-related disorders treatment.
34010697	7	7	theme	survival	842:849	arg1	rate					851:854	The survival rate	838:854	The survival rate	838:854	The survival rate was obtained by feeding a vial containing five layers of filter paper, which was infiltrated with the 5% sucrose solution contaminated with SDS or SDS polysaccharide.
34010697	8	8	theme	real-time	1221:1229	arg1	qRT-PCR					1249:1255	qRT-PCR	1249:1255	qRT-PCR	1249:1255	The microvilli and nucleus of the midgut epithelial cells of different treatments were observed by transmission electron microscope, and the expression of inflammation-related genes was detected by real-time quantitative PCR (qRT-PCR).
34010697	8	8	theme	real-time	1221:1229	arg1	PCR					1244:1246	real-time quantitative PCR	1221:1246	real-time quantitative PCR (qRT-PCR)	1221:1256	The microvilli and nucleus of the midgut epithelial cells of different treatments were observed by transmission electron microscope, and the expression of inflammation-related genes was detected by real-time quantitative PCR (qRT-PCR).
34010697	10	9	theme	current	1395:1401	arg1	study					1403:1407	the current study	1391:1407	the current study	1391:1407	RESULTS In the current study, we showed that pPMTLs significantly prolonged the life span of SDS-inflamed flies from 5 days to 6 days.
34010697	6	10	theme	treatment	754:762	arg1	group					764:768	The treatment group	750:768	The treatment group	750:768	The treatment group contained Premna microphylla turcz polysaccharide (pPMTLs) extract.
34010697	2	11	theme	detoxification	305:318	arg1	functions					292:300	the functions	288:300	the functions of detoxification, swelling and hemostasis	288:343	Its roots, stems and leaves can be invoked as medicines, which have the functions of detoxification, swelling and hemostasis.
34010697	11	12	from	rupture	1538:1544	arg1	midgut					1567:1572	the midgut	1563:1572	the midgut	1563:1572	And pPMTLs reduced the rupture of microvilli in the midgut and restored the nuclear structure.
34010697	8	13	theme	genes	1199:1203	arg1	expression					1164:1173	the expression	1160:1173	the expression of inflammation-related genes	1160:1203	The microvilli and nucleus of the midgut epithelial cells of different treatments were observed by transmission electron microscope, and the expression of inflammation-related genes was detected by real-time quantitative PCR (qRT-PCR).
34010697	14	14	theme	disorders	2148:2156	arg1	treatment					2158:2166	the inflammation-related disorders treatment	2123:2166	the inflammation-related disorders treatment	2123:2166	CONCLUSIONS Collectively, our results revealed the potential application of pPMTLs in enhancing inflammation defense, which would be enormous significance for the inflammation-related disorders treatment.
34010697	5	15	theme	standard	663:670	arg1	medium					689:694	standard corn flour-yeast medium	663:694	standard corn flour-yeast medium	663:694	MATERIALS AND METHODS The flies were fed with standard corn flour-yeast medium to cause inflammation by sodium lauryl sulfate (SDS).
34010697	7	16	theme	sucrose	961:967	arg1	solution					969:976	the 5% sucrose solution	954:976	the 5% sucrose solution contaminated with SDS or SDS polysaccharide	954:1020	The survival rate was obtained by feeding a vial containing five layers of filter paper, which was infiltrated with the 5% sucrose solution contaminated with SDS or SDS polysaccharide.
34010697	1	17	theme	RELEVANCE	145:153	arg1	remedy					212:217	a folk remedy	205:217	a folk remedy	205:217	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz is traditionally used as a folk remedy.
34010697	1	17	theme	RELEVANCE	145:153	arg1	turcz					174:178	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz	124:178	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz	124:178	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz is traditionally used as a folk remedy.
34010697	0	18	from	host	118:121	arg1	enhancement					78:88	the enhancement	74:88	the enhancement of intestinal resistance in host	74:121	Polysaccharides from Premna microphylla turcz ameliorate inflammation via the enhancement of intestinal resistance in host.
34010697	12	19	dep	Drosophila	1710:1719	arg1	defensin					1736:1743	the defensin	1732:1743	Inflammation Drosophila especially the defensin (4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group: SDS group, p < 0.001)	1697:1819	In addition, pPMTLs significantly improved expression level of immune-related genes in Inflammation Drosophila especially the defensin (4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group: SDS group, p < 0.001).
34010697	12	19	dep	Drosophila	1710:1719	arg1	group					1792:1796	4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group	1746:1796	4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group	1746:1796	In addition, pPMTLs significantly improved expression level of immune-related genes in Inflammation Drosophila especially the defensin (4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group: SDS group, p < 0.001).
34010697	5	20	theme	flour-yeast	677:687	arg1	medium					689:694	standard corn flour-yeast medium	663:694	standard corn flour-yeast medium	663:694	MATERIALS AND METHODS The flies were fed with standard corn flour-yeast medium to cause inflammation by sodium lauryl sulfate (SDS).
34010697	7	21	theme	5	958:958	arg1	%					959:959	%	959:959	%	959:959	The survival rate was obtained by feeding a vial containing five layers of filter paper, which was infiltrated with the 5% sucrose solution contaminated with SDS or SDS polysaccharide.
34010697	1	22	theme	microphylla	162:172	arg1	remedy					212:217	a folk remedy	205:217	a folk remedy	205:217	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz is traditionally used as a folk remedy.
34010697	1	22	theme	microphylla	162:172	arg1	turcz					174:178	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz	124:178	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz	124:178	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz is traditionally used as a folk remedy.
34010697	12	23	theme	genes	1688:1692	arg1	level					1664:1668	expression level	1653:1668	expression level of immune-related genes in Inflammation Drosophila especially the defensin (4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group: SDS group, p < 0.001)	1653:1819	In addition, pPMTLs significantly improved expression level of immune-related genes in Inflammation Drosophila especially the defensin (4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group: SDS group, p < 0.001).
34010697	0	24	from	resistance	104:113	arg1	host					118:121	host	118:121	host	118:121	Polysaccharides from Premna microphylla turcz ameliorate inflammation via the enhancement of intestinal resistance in host.
34010697	13	25	theme	relative	1893:1900	arg1	abundance					1902:1910	the relative abundance	1889:1910	the relative abundance of Raoultella	1889:1924	The analysis of intestinal microbiota showed that pPMTLs decreased the relative abundance of Raoultella while Wolbachia increased (p < 0.05).
34010697	14	26	theme	inflammation-related	2127:2146	arg1	treatment					2158:2166	the inflammation-related disorders treatment	2123:2166	the inflammation-related disorders treatment	2123:2166	CONCLUSIONS Collectively, our results revealed the potential application of pPMTLs in enhancing inflammation defense, which would be enormous significance for the inflammation-related disorders treatment.
34010697	9	27	theme	16S	1268:1270	arg1	analysis					1277:1284	16S rDNA analysis	1268:1284	16S rDNA analysis	1268:1284	Finally, 16S rDNA analysis was conducted on the differences in the composition of the intestinal microbes of Drosophila.
34010697	12	28	theme	expression	1653:1662	arg1	level					1664:1668	expression level	1653:1668	expression level of immune-related genes in Inflammation Drosophila especially the defensin (4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group: SDS group, p < 0.001)	1653:1819	In addition, pPMTLs significantly improved expression level of immune-related genes in Inflammation Drosophila especially the defensin (4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group: SDS group, p < 0.001).
34010697	4	29	theme	in-depth	490:497	arg1	studies					499:505	in-depth studies	490:505	in-depth studies	490:505	However, there are few reports of in-depth studies on the anti-inflammatory effects of polysaccharide, which was the main component in Premna microphylla turcz.
34010697	0	30	theme	resistance	104:113	arg1	enhancement					78:88	the enhancement	74:88	the enhancement of intestinal resistance in host	74:121	Polysaccharides from Premna microphylla turcz ameliorate inflammation via the enhancement of intestinal resistance in host.
34010697	3	31	from	Premna	364:369	arg1	Verbenaceae					378:388	the Verbenaceae	374:388	the Verbenaceae	374:388	It belongs to the Premna in the Verbenaceae and is mainly distributed in the mountains of southeastern China.
34010697	14	32	theme	enormous	2097:2104	arg1	significance					2106:2117	enormous significance	2097:2117	enormous significance	2097:2117	CONCLUSIONS Collectively, our results revealed the potential application of pPMTLs in enhancing inflammation defense, which would be enormous significance for the inflammation-related disorders treatment.
34010697	7	33	theme	filter	913:918	arg1	paper					920:924	filter paper	913:924	filter paper	913:924	The survival rate was obtained by feeding a vial containing five layers of filter paper, which was infiltrated with the 5% sucrose solution contaminated with SDS or SDS polysaccharide.
34010697	0	34	from	turcz	40:44	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from Premna microphylla turcz	0:44	Polysaccharides from Premna microphylla turcz ameliorate inflammation via the enhancement of intestinal resistance in host.
34010697	13	35	theme	microbiota	1849:1858	arg1	analysis					1826:1833	The analysis	1822:1833	The analysis of intestinal microbiota	1822:1858	The analysis of intestinal microbiota showed that pPMTLs decreased the relative abundance of Raoultella while Wolbachia increased (p < 0.05).
34010697	14	36	theme	inflammation	2060:2071	arg1	defense					2073:2079	inflammation defense	2060:2079	inflammation defense	2060:2079	CONCLUSIONS Collectively, our results revealed the potential application of pPMTLs in enhancing inflammation defense, which would be enormous significance for the inflammation-related disorders treatment.
34010697	9	37	from	differences	1307:1317	arg1	composition					1326:1336	the composition	1322:1336	the composition of the intestinal microbes of Drosophila	1322:1377	Finally, 16S rDNA analysis was conducted on the differences in the composition of the intestinal microbes of Drosophila.
34010697	0	38	theme	Premna	21:26	arg1	turcz					40:44	Premna microphylla turcz	21:44	Premna microphylla turcz	21:44	Polysaccharides from Premna microphylla turcz ameliorate inflammation via the enhancement of intestinal resistance in host.
34010697	2	39	contain	have	283:286	arg1	leaves					241:246	leaves	241:246	leaves	241:246	Its roots, stems and leaves can be invoked as medicines, which have the functions of detoxification, swelling and hemostasis.
34010697	2	39	contain	have	283:286	arg1	stems					231:235	stems	231:235	stems	231:235	Its roots, stems and leaves can be invoked as medicines, which have the functions of detoxification, swelling and hemostasis.
34010697	2	39	contain	have	283:286	arg1	roots					224:228	Its roots	220:228	Its roots	220:228	Its roots, stems and leaves can be invoked as medicines, which have the functions of detoxification, swelling and hemostasis.
34010697	2	39	contain	have	283:286	arg2	functions					292:300	the functions	288:300	the functions of detoxification, swelling and hemostasis	288:343	Its roots, stems and leaves can be invoked as medicines, which have the functions of detoxification, swelling and hemostasis.
34010697	2	39	contain	have	283:286	arg1	medicines					266:274	medicines	266:274	medicines	266:274	Its roots, stems and leaves can be invoked as medicines, which have the functions of detoxification, swelling and hemostasis.
34010697	10	40	theme	life	1460:1463	arg1	span					1465:1468	the life span	1456:1468	the life span of SDS-inflamed flies from 5 days to 6 days	1456:1512	RESULTS In the current study, we showed that pPMTLs significantly prolonged the life span of SDS-inflamed flies from 5 days to 6 days.
34010697	3	41	theme	southeastern	436:447	arg1	China					449:453	southeastern China	436:453	southeastern China	436:453	It belongs to the Premna in the Verbenaceae and is mainly distributed in the mountains of southeastern China.
34010697	12	42	theme	SDS	1799:1801	arg1	group					1803:1807	SDS group	1799:1807	SDS group	1799:1807	In addition, pPMTLs significantly improved expression level of immune-related genes in Inflammation Drosophila especially the defensin (4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group: SDS group, p < 0.001).
34010697	11	43	theme	microvilli	1549:1558	arg1	rupture					1538:1544	the rupture	1534:1544	the rupture of microvilli in the midgut	1534:1572	And pPMTLs reduced the rupture of microvilli in the midgut and restored the nuclear structure.
34010697	8	44	theme	epithelial	1064:1073	arg1	cells					1075:1079	the midgut epithelial cells	1053:1079	the midgut epithelial cells of different treatments	1053:1103	The microvilli and nucleus of the midgut epithelial cells of different treatments were observed by transmission electron microscope, and the expression of inflammation-related genes was detected by real-time quantitative PCR (qRT-PCR).
34010697	4	45	theme	anti-inflammatory	514:530	arg1	effects					532:538	the anti-inflammatory effects	510:538	the anti-inflammatory effects of polysaccharide, which was the main component in Premna microphylla turcz	510:614	However, there are few reports of in-depth studies on the anti-inflammatory effects of polysaccharide, which was the main component in Premna microphylla turcz.
34010697	10	46	theme	flies	1486:1490	arg1	span					1465:1468	the life span	1456:1468	the life span of SDS-inflamed flies from 5 days to 6 days	1456:1512	RESULTS In the current study, we showed that pPMTLs significantly prolonged the life span of SDS-inflamed flies from 5 days to 6 days.
34010697	13	47	theme	Raoultella	1915:1924	arg1	abundance					1902:1910	the relative abundance	1889:1910	the relative abundance of Raoultella	1889:1924	The analysis of intestinal microbiota showed that pPMTLs decreased the relative abundance of Raoultella while Wolbachia increased (p < 0.05).
34010697	10	48	dep	RESULTS	1380:1386	arg1	showed					1413:1418	showed	1413:1418	showed that pPMTLs significantly prolonged the life span of SDS-inflamed flies from 5 days to 6 days	1413:1512	RESULTS In the current study, we showed that pPMTLs significantly prolonged the life span of SDS-inflamed flies from 5 days to 6 days.
34010697	9	49	theme	microbes	1356:1363	arg1	composition					1326:1336	the composition	1322:1336	the composition of the intestinal microbes of Drosophila	1322:1377	Finally, 16S rDNA analysis was conducted on the differences in the composition of the intestinal microbes of Drosophila.
34010697	6	50	theme	pPMTLs	821:826	arg1	extract					829:835	Premna microphylla turcz polysaccharide (pPMTLs) extract	780:835	Premna microphylla turcz polysaccharide (pPMTLs) extract	780:835	The treatment group contained Premna microphylla turcz polysaccharide (pPMTLs) extract.
34010697	9	51	theme	Drosophila	1368:1377	arg1	microbes					1356:1363	the intestinal microbes	1341:1363	the intestinal microbes of Drosophila	1341:1377	Finally, 16S rDNA analysis was conducted on the differences in the composition of the intestinal microbes of Drosophila.
34010697	8	52	theme	treatments	1094:1103	arg1	cells					1075:1079	the midgut epithelial cells	1053:1079	the midgut epithelial cells of different treatments	1053:1103	The microvilli and nucleus of the midgut epithelial cells of different treatments were observed by transmission electron microscope, and the expression of inflammation-related genes was detected by real-time quantitative PCR (qRT-PCR).
34010697	4	53	theme	microphylla	598:608	arg1	turcz					610:614	Premna microphylla turcz	591:614	Premna microphylla turcz	591:614	However, there are few reports of in-depth studies on the anti-inflammatory effects of polysaccharide, which was the main component in Premna microphylla turcz.
34010697	6	54	theme	turcz	799:803	arg1	extract					829:835	Premna microphylla turcz polysaccharide (pPMTLs) extract	780:835	Premna microphylla turcz polysaccharide (pPMTLs) extract	780:835	The treatment group contained Premna microphylla turcz polysaccharide (pPMTLs) extract.
34010697	8	55	theme	transmission	1122:1133	arg1	microscope					1144:1153	transmission electron microscope	1122:1153	transmission electron microscope	1122:1153	The microvilli and nucleus of the midgut epithelial cells of different treatments were observed by transmission electron microscope, and the expression of inflammation-related genes was detected by real-time quantitative PCR (qRT-PCR).
34010697	4	56	theme	main	573:576	arg1	polysaccharide					543:556	polysaccharide	543:556	polysaccharide	543:556	However, there are few reports of in-depth studies on the anti-inflammatory effects of polysaccharide, which was the main component in Premna microphylla turcz.
34010697	4	56	theme	main	573:576	arg1	component					578:586	the main component	569:586	the main component in Premna microphylla turcz	569:614	However, there are few reports of in-depth studies on the anti-inflammatory effects of polysaccharide, which was the main component in Premna microphylla turcz.
34010697	6	57	theme	Premna	780:785	arg1	extract					829:835	Premna microphylla turcz polysaccharide (pPMTLs) extract	780:835	Premna microphylla turcz polysaccharide (pPMTLs) extract	780:835	The treatment group contained Premna microphylla turcz polysaccharide (pPMTLs) extract.
34010697	12	58	theme	Inflammation	1697:1708	arg1	Drosophila					1710:1719	Inflammation Drosophila	1697:1719	Inflammation Drosophila especially the defensin (4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group: SDS group, p < 0.001)	1697:1819	In addition, pPMTLs significantly improved expression level of immune-related genes in Inflammation Drosophila especially the defensin (4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group: SDS group, p < 0.001).
34010697	1	59	theme	folk	207:210	arg1	remedy					212:217	a folk remedy	205:217	a folk remedy	205:217	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz is traditionally used as a folk remedy.
34010697	1	59	theme	folk	207:210	arg1	turcz					174:178	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz	124:178	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz	124:178	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz is traditionally used as a folk remedy.
34010697	8	60	theme	quantitative	1231:1242	arg1	qRT-PCR					1249:1255	qRT-PCR	1249:1255	qRT-PCR	1249:1255	The microvilli and nucleus of the midgut epithelial cells of different treatments were observed by transmission electron microscope, and the expression of inflammation-related genes was detected by real-time quantitative PCR (qRT-PCR).
34010697	8	60	theme	quantitative	1231:1242	arg1	PCR					1244:1246	real-time quantitative PCR	1221:1246	real-time quantitative PCR (qRT-PCR)	1221:1256	The microvilli and nucleus of the midgut epithelial cells of different treatments were observed by transmission electron microscope, and the expression of inflammation-related genes was detected by real-time quantitative PCR (qRT-PCR).
34010697	8	61	theme	inflammation-related	1178:1197	arg1	genes					1199:1203	inflammation-related genes	1178:1203	inflammation-related genes	1178:1203	The microvilli and nucleus of the midgut epithelial cells of different treatments were observed by transmission electron microscope, and the expression of inflammation-related genes was detected by real-time quantitative PCR (qRT-PCR).
34010697	0	62	from	enhancement	78:88	arg1	host					118:121	host	118:121	host	118:121	Polysaccharides from Premna microphylla turcz ameliorate inflammation via the enhancement of intestinal resistance in host.
34010697	7	63	theme	SDS	1003:1005	arg1	polysaccharide					1007:1020	SDS polysaccharide	1003:1020	SDS polysaccharide	1003:1020	The survival rate was obtained by feeding a vial containing five layers of filter paper, which was infiltrated with the 5% sucrose solution contaminated with SDS or SDS polysaccharide.
34010697	1	64	theme	ETHNOPHARMACOLOGICAL	124:143	arg1	remedy					212:217	a folk remedy	205:217	a folk remedy	205:217	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz is traditionally used as a folk remedy.
34010697	1	64	theme	ETHNOPHARMACOLOGICAL	124:143	arg1	turcz					174:178	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz	124:178	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz	124:178	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz is traditionally used as a folk remedy.
34010697	12	65	from	level	1664:1668	arg1	Drosophila					1710:1719	Inflammation Drosophila	1697:1719	Inflammation Drosophila especially the defensin (4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group: SDS group, p < 0.001)	1697:1819	In addition, pPMTLs significantly improved expression level of immune-related genes in Inflammation Drosophila especially the defensin (4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group: SDS group, p < 0.001).
34010697	5	66	theme	corn	672:675	arg1	medium					689:694	standard corn flour-yeast medium	663:694	standard corn flour-yeast medium	663:694	MATERIALS AND METHODS The flies were fed with standard corn flour-yeast medium to cause inflammation by sodium lauryl sulfate (SDS).
34010697	1	67	theme	Premna	155:160	arg1	remedy					212:217	a folk remedy	205:217	a folk remedy	205:217	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz is traditionally used as a folk remedy.
34010697	1	67	theme	Premna	155:160	arg1	turcz					174:178	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz	124:178	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz	124:178	ETHNOPHARMACOLOGICAL RELEVANCE Premna microphylla turcz is traditionally used as a folk remedy.
34010697	7	68	theme	%	959:959	arg1	solution					969:976	the 5% sucrose solution	954:976	the 5% sucrose solution contaminated with SDS or SDS polysaccharide	954:1020	The survival rate was obtained by feeding a vial containing five layers of filter paper, which was infiltrated with the 5% sucrose solution contaminated with SDS or SDS polysaccharide.
34010697	12	69	theme	immune-related	1673:1686	arg1	genes					1688:1692	immune-related genes	1673:1692	immune-related genes	1673:1692	In addition, pPMTLs significantly improved expression level of immune-related genes in Inflammation Drosophila especially the defensin (4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group: SDS group, p < 0.001).
34010697	5	70	theme	sodium	721:726	arg1	SDS					744:746	SDS	744:746	SDS	744:746	MATERIALS AND METHODS The flies were fed with standard corn flour-yeast medium to cause inflammation by sodium lauryl sulfate (SDS).
34010697	5	70	theme	sodium	721:726	arg1	sulfate					735:741	sodium lauryl sulfate	721:741	sodium lauryl sulfate (SDS)	721:747	MATERIALS AND METHODS The flies were fed with standard corn flour-yeast medium to cause inflammation by sodium lauryl sulfate (SDS).
34010697	0	71	theme	intestinal	93:102	arg1	resistance					104:113	intestinal resistance	93:113	intestinal resistance in host	93:121	Polysaccharides from Premna microphylla turcz ameliorate inflammation via the enhancement of intestinal resistance in host.
34010697	13	72	dep	increased	1942:1950	arg1	p < 0.05					1953:1960	p < 0.05	1953:1960	p < 0.05	1953:1960	The analysis of intestinal microbiota showed that pPMTLs decreased the relative abundance of Raoultella while Wolbachia increased (p < 0.05).
34010697	4	73	theme	studies	499:505	arg1	reports					479:485	few reports	475:485	few reports of in-depth studies on the anti-inflammatory effects of polysaccharide, which was the main component in Premna microphylla turcz	475:614	However, there are few reports of in-depth studies on the anti-inflammatory effects of polysaccharide, which was the main component in Premna microphylla turcz.
34010697	4	74	from	component	578:586	arg1	turcz					610:614	Premna microphylla turcz	591:614	Premna microphylla turcz	591:614	However, there are few reports of in-depth studies on the anti-inflammatory effects of polysaccharide, which was the main component in Premna microphylla turcz.
34010697	9	75	theme	rDNA	1272:1275	arg1	analysis					1277:1284	16S rDNA analysis	1268:1284	16S rDNA analysis	1268:1284	Finally, 16S rDNA analysis was conducted on the differences in the composition of the intestinal microbes of Drosophila.
34010697	14	76	theme	potential	2015:2023	arg1	application					2025:2035	the potential application	2011:2035	the potential application of pPMTLs in enhancing inflammation defense, which would be enormous significance for the inflammation-related disorders treatment	2011:2166	CONCLUSIONS Collectively, our results revealed the potential application of pPMTLs in enhancing inflammation defense, which would be enormous significance for the inflammation-related disorders treatment.
34010697	12	77	dep	group	1792:1796	arg1	p < 0.001					1810:1818	p < 0.001	1810:1818	p < 0.001	1810:1818	In addition, pPMTLs significantly improved expression level of immune-related genes in Inflammation Drosophila especially the defensin (4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group: SDS group, p < 0.001).
34010697	12	77	dep	group	1792:1796	arg1	group					1803:1807	SDS group	1799:1807	SDS group	1799:1807	In addition, pPMTLs significantly improved expression level of immune-related genes in Inflammation Drosophila especially the defensin (4.32 ± 0.75 vs 9.97 ± 0.52 SDS-polysaccharide group: SDS group, p < 0.001).
34010697	5	78	theme	lauryl	728:733	arg1	SDS					744:746	SDS	744:746	SDS	744:746	MATERIALS AND METHODS The flies were fed with standard corn flour-yeast medium to cause inflammation by sodium lauryl sulfate (SDS).
34010697	5	78	theme	lauryl	728:733	arg1	sulfate					735:741	sodium lauryl sulfate	721:741	sodium lauryl sulfate (SDS)	721:747	MATERIALS AND METHODS The flies were fed with standard corn flour-yeast medium to cause inflammation by sodium lauryl sulfate (SDS).
34010697	6	79	contain	contained	770:778	arg1	group					764:768	The treatment group	750:768	The treatment group	750:768	The treatment group contained Premna microphylla turcz polysaccharide (pPMTLs) extract.
34010697	6	79	contain	contained	770:778	arg2	extract					829:835	Premna microphylla turcz polysaccharide (pPMTLs) extract	780:835	Premna microphylla turcz polysaccharide (pPMTLs) extract	780:835	The treatment group contained Premna microphylla turcz polysaccharide (pPMTLs) extract.
34010697	4	80	theme	few	475:477	arg1	reports					479:485	few reports	475:485	few reports of in-depth studies on the anti-inflammatory effects of polysaccharide, which was the main component in Premna microphylla turcz	475:614	However, there are few reports of in-depth studies on the anti-inflammatory effects of polysaccharide, which was the main component in Premna microphylla turcz.
34010697	13	81	theme	intestinal	1838:1847	arg1	microbiota					1849:1858	intestinal microbiota	1838:1858	intestinal microbiota	1838:1858	The analysis of intestinal microbiota showed that pPMTLs decreased the relative abundance of Raoultella while Wolbachia increased (p < 0.05).
34010697	7	82	theme	paper	920:924	arg1	layers					903:908	five layers	898:908	five layers of filter paper	898:924	The survival rate was obtained by feeding a vial containing five layers of filter paper, which was infiltrated with the 5% sucrose solution contaminated with SDS or SDS polysaccharide.
34010697	4	83	theme	polysaccharide	543:556	arg1	effects					532:538	the anti-inflammatory effects	510:538	the anti-inflammatory effects of polysaccharide, which was the main component in Premna microphylla turcz	510:614	However, there are few reports of in-depth studies on the anti-inflammatory effects of polysaccharide, which was the main component in Premna microphylla turcz.
34010697	7	84	contain	containing	887:896	arg1	vial					882:885	a vial	880:885	a vial	880:885	The survival rate was obtained by feeding a vial containing five layers of filter paper, which was infiltrated with the 5% sucrose solution contaminated with SDS or SDS polysaccharide.
34010697	7	84	contain	containing	887:896	arg2	layers					903:908	five layers	898:908	five layers of filter paper	898:924	The survival rate was obtained by feeding a vial containing five layers of filter paper, which was infiltrated with the 5% sucrose solution contaminated with SDS or SDS polysaccharide.
34010697	0	85	theme	microphylla	28:38	arg1	turcz					40:44	Premna microphylla turcz	21:44	Premna microphylla turcz	21:44	Polysaccharides from Premna microphylla turcz ameliorate inflammation via the enhancement of intestinal resistance in host.
34010697	8	86	theme	midgut	1057:1062	arg1	cells					1075:1079	the midgut epithelial cells	1053:1079	the midgut epithelial cells of different treatments	1053:1103	The microvilli and nucleus of the midgut epithelial cells of different treatments were observed by transmission electron microscope, and the expression of inflammation-related genes was detected by real-time quantitative PCR (qRT-PCR).
34010697	10	87	from	days	1499:1502	arg1	span					1465:1468	the life span	1456:1468	the life span of SDS-inflamed flies from 5 days to 6 days	1456:1512	RESULTS In the current study, we showed that pPMTLs significantly prolonged the life span of SDS-inflamed flies from 5 days to 6 days.
34010697	3	88	theme	China	449:453	arg1	mountains					423:431	the mountains	419:431	the mountains of southeastern China	419:453	It belongs to the Premna in the Verbenaceae and is mainly distributed in the mountains of southeastern China.
34010697	8	89	theme	cells	1075:1079	arg1	nucleus					1042:1048	nucleus	1042:1048	nucleus	1042:1048	The microvilli and nucleus of the midgut epithelial cells of different treatments were observed by transmission electron microscope, and the expression of inflammation-related genes was detected by real-time quantitative PCR (qRT-PCR).
34010697	8	89	theme	cells	1075:1079	arg1	microvilli					1027:1036	microvilli	1027:1036	microvilli	1027:1036	The microvilli and nucleus of the midgut epithelial cells of different treatments were observed by transmission electron microscope, and the expression of inflammation-related genes was detected by real-time quantitative PCR (qRT-PCR).
34010697	5	90	dep	MATERIALS	617:625	arg1	flies					643:647	The flies	639:647	The flies	639:647	MATERIALS AND METHODS The flies were fed with standard corn flour-yeast medium to cause inflammation by sodium lauryl sulfate (SDS).
34010697	14	91	theme	pPMTLs	2040:2045	arg1	application					2025:2035	the potential application	2011:2035	the potential application of pPMTLs in enhancing inflammation defense, which would be enormous significance for the inflammation-related disorders treatment	2011:2166	CONCLUSIONS Collectively, our results revealed the potential application of pPMTLs in enhancing inflammation defense, which would be enormous significance for the inflammation-related disorders treatment.
34010697	2	92	theme	hemostasis	334:343	arg1	functions					292:300	the functions	288:300	the functions of detoxification, swelling and hemostasis	288:343	Its roots, stems and leaves can be invoked as medicines, which have the functions of detoxification, swelling and hemostasis.
34010697	9	93	theme	intestinal	1345:1354	arg1	microbes					1356:1363	the intestinal microbes	1341:1363	the intestinal microbes of Drosophila	1341:1377	Finally, 16S rDNA analysis was conducted on the differences in the composition of the intestinal microbes of Drosophila.
34010697	8	94	theme	different	1084:1092	arg1	treatments					1094:1103	different treatments	1084:1103	different treatments	1084:1103	The microvilli and nucleus of the midgut epithelial cells of different treatments were observed by transmission electron microscope, and the expression of inflammation-related genes was detected by real-time quantitative PCR (qRT-PCR).
34010697	4	95	theme	Premna	591:596	arg1	turcz					610:614	Premna microphylla turcz	591:614	Premna microphylla turcz	591:614	However, there are few reports of in-depth studies on the anti-inflammatory effects of polysaccharide, which was the main component in Premna microphylla turcz.
34010697	10	96	theme	SDS-inflamed	1473:1484	arg1	flies					1486:1490	SDS-inflamed flies	1473:1490	SDS-inflamed flies	1473:1490	RESULTS In the current study, we showed that pPMTLs significantly prolonged the life span of SDS-inflamed flies from 5 days to 6 days.
34010697	2	97	theme	swelling	321:328	arg1	functions					292:300	the functions	288:300	the functions of detoxification, swelling and hemostasis	288:343	Its roots, stems and leaves can be invoked as medicines, which have the functions of detoxification, swelling and hemostasis.
34010697	8	98	dep	microvilli	1027:1036	arg1	The					1023:1025	The	1023:1025	The	1023:1025	The microvilli and nucleus of the midgut epithelial cells of different treatments were observed by transmission electron microscope, and the expression of inflammation-related genes was detected by real-time quantitative PCR (qRT-PCR).
34010697	11	99	theme	nuclear	1591:1597	arg1	structure					1599:1607	the nuclear structure	1587:1607	the nuclear structure	1587:1607	And pPMTLs reduced the rupture of microvilli in the midgut and restored the nuclear structure.
34890854	0	0	theme	resonance	117:125	arg1	spectroscopy					127:138	nuclear magnetic resonance spectroscopy	100:138	nuclear magnetic resonance spectroscopy	100:138	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.
34890854	2	1	theme	migration	530:538	arg1	order					540:544	Opposite enantiomer migration order	510:544	Opposite enantiomer migration order	510:544	Opposite enantiomer migration order was observed for randomly substituted CDs compared to 2,6-DM-β-CD as well as methylated β-CDs with different composition according to the specifications of the manufacturers.
34890854	0	2	theme	daclatasvir	235:245	arg1	enantioseparation					214:230	the capillary electrophoresis enantioseparation	184:230	the capillary electrophoresis enantioseparation of daclatasvir	184:245	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.
34890854	4	3	theme	2,6-DM-β-CD	916:926	arg1	content					935:941	low 2,6-DM-β-CD isomer content	912:941	low 2,6-DM-β-CD isomer content	912:941	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	1	4	from	selectors	463:471	arg1	electrolyte					497:507	an acidic background electrolyte	476:507	an acidic background electrolyte	476:507	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	2	5	theme	methylated	623:632	arg1	β-CDs					634:638	methylated β-CDs	623:638	2,6-DM-β-CD as well as methylated β-CDs with different composition	600:665	Opposite enantiomer migration order was observed for randomly substituted CDs compared to 2,6-DM-β-CD as well as methylated β-CDs with different composition according to the specifications of the manufacturers.
34890854	0	6	from	enantioseparation	214:230	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.	0:246	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.
34890854	0	7	theme	magnetic	108:115	arg1	resonance					117:125	nuclear magnetic resonance	100:125	nuclear magnetic resonance spectroscopy	100:138	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.
34890854	0	8	from	characterization	11:26	arg1	enantioseparation					214:230	the capillary electrophoresis enantioseparation	184:230	the capillary electrophoresis enantioseparation of daclatasvir	184:245	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.
34890854	2	9	theme	different	645:653	arg1	composition					655:665	different composition	645:665	different composition	645:665	Opposite enantiomer migration order was observed for randomly substituted CDs compared to 2,6-DM-β-CD as well as methylated β-CDs with different composition according to the specifications of the manufacturers.
34890854	5	10	theme	derivatized	1238:1248	arg1	CDs					1250:1252	derivatized CDs	1238:1252	derivatized CDs	1238:1252	The study indicated the importance of the type and composition of derivatized CDs on chiral separations in capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers.
34890854	5	11	dep	type	1214:1217	arg1	the					1210:1212	the	1210:1212	the	1210:1212	The study indicated the importance of the type and composition of derivatized CDs on chiral separations in capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers.
34890854	2	12	theme	substituted	572:582	arg1	CDs					584:586	randomly substituted CDs	563:586	randomly substituted CDs compared to 2,6-DM-β-CD as well as methylated β-CDs with different composition according to the specifications of the manufacturers	563:718	Opposite enantiomer migration order was observed for randomly substituted CDs compared to 2,6-DM-β-CD as well as methylated β-CDs with different composition according to the specifications of the manufacturers.
34890854	2	13	theme	manufacturers	706:718	arg1	specifications					684:697	the specifications	680:697	the specifications of the manufacturers	680:718	Opposite enantiomer migration order was observed for randomly substituted CDs compared to 2,6-DM-β-CD as well as methylated β-CDs with different composition according to the specifications of the manufacturers.
34890854	5	14	theme	CDs	1250:1252	arg1	composition					1223:1233	composition	1223:1233	composition	1223:1233	The study indicated the importance of the type and composition of derivatized CDs on chiral separations in capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers.
34890854	5	14	theme	CDs	1250:1252	arg1	type					1214:1217	type	1214:1217	type	1214:1217	The study indicated the importance of the type and composition of derivatized CDs on chiral separations in capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers.
34890854	4	15	theme	CD	995:996	arg1	isomers					998:1004	methylated CD isomers	984:1004	methylated CD isomers	984:1004	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	1	16	theme	single	394:399	arg1	-β-CD					433:437	the single isomer heptakis(2,6-di-O-methyl)-β-CD	390:437	the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD)	390:451	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	1	16	theme	single	394:399	arg1	selectors					463:471	chiral selectors	456:471	chiral selectors in an acidic background electrolyte	456:507	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	1	16	theme	single	394:399	arg1	β-CDs					380:384	various randomly methylated β-CDs	352:384	various randomly methylated β-CDs	352:384	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	1	16	theme	single	394:399	arg1	2,6-DM-β-CD					440:450	2,6-DM-β-CD	440:450	2,6-DM-β-CD	440:450	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	3	17	theme	R-enantiomer	857:868	arg1	daclatasvir					872:882	R-enantiomer > daclatasvir	857:882	R-enantiomer > daclatasvir	857:882	HPLC and NMR analyses confirmed that the presence of a high 2,6-DM-β-CD content in the CDs enables to achieve the migration order R,R,R,R-enantiomer > daclatasvir.
34890854	3	17	theme	R-enantiomer	857:868	arg1	R					851:851	R	851:851	R	851:851	HPLC and NMR analyses confirmed that the presence of a high 2,6-DM-β-CD content in the CDs enables to achieve the migration order R,R,R,R-enantiomer > daclatasvir.
34890854	4	18	theme	daclatasvir	1138:1148	arg1	R-enantiomer					1158:1169	R-enantiomer	1158:1169	R-enantiomer	1158:1169	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	18	theme	daclatasvir	1138:1148	arg1	R					1156:1156	R	1156:1156	R	1156:1156	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	18	theme	daclatasvir	1138:1148	arg1	R					1154:1154	R	1154:1154	R	1154:1154	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	18	theme	daclatasvir	1138:1148	arg1	R					1152:1152	the opposite enantiomer migration order daclatasvir > R	1098:1152	the opposite enantiomer migration order daclatasvir > R	1098:1152	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	19	theme	>	1150:1150	arg1	R-enantiomer					1158:1169	R-enantiomer	1158:1169	R-enantiomer	1158:1169	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	19	theme	>	1150:1150	arg1	R					1156:1156	R	1156:1156	R	1156:1156	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	19	theme	>	1150:1150	arg1	R					1154:1154	R	1154:1154	R	1154:1154	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	19	theme	>	1150:1150	arg1	R					1152:1152	the opposite enantiomer migration order daclatasvir > R	1098:1152	the opposite enantiomer migration order daclatasvir > R	1098:1152	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	20	theme	amount	974:979	arg1	presence					954:961	the presence	950:961	the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6,	950:1086	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	1	21	theme	isomer	401:406	arg1	-β-CD					433:437	the single isomer heptakis(2,6-di-O-methyl)-β-CD	390:437	the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD)	390:451	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	1	21	theme	isomer	401:406	arg1	selectors					463:471	chiral selectors	456:471	chiral selectors in an acidic background electrolyte	456:507	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	1	21	theme	isomer	401:406	arg1	β-CDs					380:384	various randomly methylated β-CDs	352:384	various randomly methylated β-CDs	352:384	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	1	21	theme	isomer	401:406	arg1	2,6-DM-β-CD					440:450	2,6-DM-β-CD	440:450	2,6-DM-β-CD	440:450	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	1	22	theme	acidic	479:484	arg1	electrolyte					497:507	an acidic background electrolyte	476:507	an acidic background electrolyte	476:507	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	6	23	theme	observed	1403:1410	arg1	order					1422:1426	the observed migration order	1399:1426	the observed migration order	1399:1426	Moreover, the observed migration order could be rationalized based on the composition and substitution pattern of the CDs.
34890854	2	24	with	β-CDs	634:638	arg1	composition					655:665	different composition	645:665	different composition	645:665	Opposite enantiomer migration order was observed for randomly substituted CDs compared to 2,6-DM-β-CD as well as methylated β-CDs with different composition according to the specifications of the manufacturers.
34890854	0	25	theme	effect	144:149	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.	0:246	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.
34890854	1	26	theme	heptakis	408:415	arg1	-β-CD					433:437	the single isomer heptakis(2,6-di-O-methyl)-β-CD	390:437	the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD)	390:451	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	1	26	theme	heptakis	408:415	arg1	selectors					463:471	chiral selectors	456:471	chiral selectors in an acidic background electrolyte	456:507	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	1	26	theme	heptakis	408:415	arg1	β-CDs					380:384	various randomly methylated β-CDs	352:384	various randomly methylated β-CDs	352:384	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	1	26	theme	heptakis	408:415	arg1	2,6-DM-β-CD					440:450	2,6-DM-β-CD	440:450	2,6-DM-β-CD	440:450	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	2	27	theme	Opposite	510:517	arg1	order					540:544	Opposite enantiomer migration order	510:544	Opposite enantiomer migration order	510:544	Opposite enantiomer migration order was observed for randomly substituted CDs compared to 2,6-DM-β-CD as well as methylated β-CDs with different composition according to the specifications of the manufacturers.
34890854	1	28	theme	background	486:495	arg1	electrolyte					497:507	an acidic background electrolyte	476:507	an acidic background electrolyte	476:507	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	5	29	theme	chiral	1257:1262	arg1	separations					1264:1274	chiral separations	1257:1274	chiral separations in capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers	1257:1386	The study indicated the importance of the type and composition of derivatized CDs on chiral separations in capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers.
34890854	6	30	theme	migration	1412:1420	arg1	order					1422:1426	the observed migration order	1399:1426	the observed migration order	1399:1426	Moreover, the observed migration order could be rationalized based on the composition and substitution pattern of the CDs.
34890854	2	31	theme	enantiomer	519:528	arg1	order					540:544	Opposite enantiomer migration order	510:544	Opposite enantiomer migration order	510:544	Opposite enantiomer migration order was observed for randomly substituted CDs compared to 2,6-DM-β-CD as well as methylated β-CDs with different composition according to the specifications of the manufacturers.
34890854	4	32	theme	methylated	984:993	arg1	isomers					998:1004	methylated CD isomers	984:1004	methylated CD isomers	984:1004	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	5	33	from	separations	1264:1274	arg1	importance					1320:1329	the importance	1316:1329	capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers	1279:1386	The study indicated the importance of the type and composition of derivatized CDs on chiral separations in capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers.
34890854	5	33	from	separations	1264:1274	arg1	electrophoresis					1289:1303	capillary electrophoresis	1279:1303	capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers	1279:1386	The study indicated the importance of the type and composition of derivatized CDs on chiral separations in capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers.
34890854	3	34	theme	2,6-DM-β-CD	781:791	arg1	content					793:799	a high 2,6-DM-β-CD content	774:799	a high 2,6-DM-β-CD content	774:799	HPLC and NMR analyses confirmed that the presence of a high 2,6-DM-β-CD content in the CDs enables to achieve the migration order R,R,R,R-enantiomer > daclatasvir.
34890854	0	35	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.	0:246	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.
34890854	4	36	theme	opposite	1102:1109	arg1	R-enantiomer					1158:1169	R-enantiomer	1158:1169	R-enantiomer	1158:1169	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	36	theme	opposite	1102:1109	arg1	R					1156:1156	R	1156:1156	R	1156:1156	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	36	theme	opposite	1102:1109	arg1	R					1154:1154	R	1154:1154	R	1154:1154	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	36	theme	opposite	1102:1109	arg1	R					1152:1152	the opposite enantiomer migration order daclatasvir > R	1098:1152	the opposite enantiomer migration order daclatasvir > R	1098:1152	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	0	37	theme	methyl-β-cyclodextrins	31:52	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.	0:246	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.
34890854	0	38	theme	isomeric	160:167	arg1	composition					169:179	their isomeric composition	154:179	their isomeric composition	154:179	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.
34890854	4	39	theme	isomers	998:1004	arg1	amount					974:979	a large amount	966:979	a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6,	966:1086	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	39	theme	isomers	998:1004	arg1	isomers					998:1004	methylated CD isomers	984:1004	methylated CD isomers	984:1004	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	3	40	theme	content	793:799	arg1	presence					762:769	the presence	758:769	the presence of a high 2,6-DM-β-CD content in the CDs	758:810	HPLC and NMR analyses confirmed that the presence of a high 2,6-DM-β-CD content in the CDs enables to achieve the migration order R,R,R,R-enantiomer > daclatasvir.
34890854	5	41	from	importance	1196:1205	arg1	separations					1264:1274	chiral separations	1257:1274	chiral separations in capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers	1257:1386	The study indicated the importance of the type and composition of derivatized CDs on chiral separations in capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers.
34890854	1	42	theme	2,6-di-O-methyl	417:431	arg1	-β-CD					433:437	the single isomer heptakis(2,6-di-O-methyl)-β-CD	390:437	the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD)	390:451	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	1	42	theme	2,6-di-O-methyl	417:431	arg1	selectors					463:471	chiral selectors	456:471	chiral selectors in an acidic background electrolyte	456:507	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	1	42	theme	2,6-di-O-methyl	417:431	arg1	β-CDs					380:384	various randomly methylated β-CDs	352:384	various randomly methylated β-CDs	352:384	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	1	42	theme	2,6-di-O-methyl	417:431	arg1	2,6-DM-β-CD					440:450	2,6-DM-β-CD	440:450	2,6-DM-β-CD	440:450	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	4	43	theme	migration	1122:1130	arg1	R-enantiomer					1158:1169	R-enantiomer	1158:1169	R-enantiomer	1158:1169	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	43	theme	migration	1122:1130	arg1	R					1156:1156	R	1156:1156	R	1156:1156	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	43	theme	migration	1122:1130	arg1	R					1154:1154	R	1154:1154	R	1154:1154	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	43	theme	migration	1122:1130	arg1	R					1152:1152	the opposite enantiomer migration order daclatasvir > R	1098:1152	the opposite enantiomer migration order daclatasvir > R	1098:1152	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	5	44	theme	capillary	1279:1287	arg1	electrophoresis					1289:1303	capillary electrophoresis	1279:1303	capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers	1279:1386	The study indicated the importance of the type and composition of derivatized CDs on chiral separations in capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers.
34890854	0	45	theme	high-performance	57:72	arg1	chromatography					81:94	high-performance liquid chromatography	57:94	high-performance liquid chromatography	57:94	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.
34890854	1	46	theme	capillary	320:328	arg1	electrophoresis					330:344	capillary electrophoresis	320:344	capillary electrophoresis	320:344	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	3	47	dep	order	845:849	arg1	R					851:851	R	851:851	R	851:851	HPLC and NMR analyses confirmed that the presence of a high 2,6-DM-β-CD content in the CDs enables to achieve the migration order R,R,R,R-enantiomer > daclatasvir.
34890854	3	47	dep	order	845:849	arg1	daclatasvir					872:882	R-enantiomer > daclatasvir	857:882	R-enantiomer > daclatasvir	857:882	HPLC and NMR analyses confirmed that the presence of a high 2,6-DM-β-CD content in the CDs enables to achieve the migration order R,R,R,R-enantiomer > daclatasvir.
34890854	3	47	dep	order	845:849	arg1	R					855:855	R	855:855	R	855:855	HPLC and NMR analyses confirmed that the presence of a high 2,6-DM-β-CD content in the CDs enables to achieve the migration order R,R,R,R-enantiomer > daclatasvir.
34890854	3	47	dep	order	845:849	arg1	R					853:853	R	853:853	R	853:853	HPLC and NMR analyses confirmed that the presence of a high 2,6-DM-β-CD content in the CDs enables to achieve the migration order R,R,R,R-enantiomer > daclatasvir.
34890854	3	48	theme	high	776:779	arg1	content					793:799	a high 2,6-DM-β-CD content	774:799	a high 2,6-DM-β-CD content	774:799	HPLC and NMR analyses confirmed that the presence of a high 2,6-DM-β-CD content in the CDs enables to achieve the migration order R,R,R,R-enantiomer > daclatasvir.
34890854	4	49	theme	enantiomer	1111:1120	arg1	R-enantiomer					1158:1169	R-enantiomer	1158:1169	R-enantiomer	1158:1169	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	49	theme	enantiomer	1111:1120	arg1	R					1156:1156	R	1156:1156	R	1156:1156	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	49	theme	enantiomer	1111:1120	arg1	R					1154:1154	R	1154:1154	R	1154:1154	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	49	theme	enantiomer	1111:1120	arg1	R					1152:1152	the opposite enantiomer migration order daclatasvir > R	1098:1152	the opposite enantiomer migration order daclatasvir > R	1098:1152	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	1	50	theme	daclatasvir	266:276	arg1	separation					252:261	The separation	248:261	The separation of daclatasvir and its R,R,R,R-enantiomer	248:303	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	4	51	theme	order	1132:1136	arg1	R-enantiomer					1158:1169	R-enantiomer	1158:1169	R-enantiomer	1158:1169	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	51	theme	order	1132:1136	arg1	R					1156:1156	R	1156:1156	R	1156:1156	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	51	theme	order	1132:1136	arg1	R					1154:1154	R	1154:1154	R	1154:1154	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	51	theme	order	1132:1136	arg1	R					1152:1152	the opposite enantiomer migration order daclatasvir > R	1098:1152	the opposite enantiomer migration order daclatasvir > R	1098:1152	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	0	52	theme	composition	169:179	arg1	methyl-β-cyclodextrins					31:52	methyl-β-cyclodextrins	31:52	methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy	31:138	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.
34890854	0	52	theme	composition	169:179	arg1	effect					144:149	effect	144:149	effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir	144:245	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.
34890854	5	53	theme	type	1214:1217	arg1	importance					1196:1205	the importance	1192:1205	the importance of the type and composition of derivatized CDs on chiral separations in capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers	1192:1386	The study indicated the importance of the type and composition of derivatized CDs on chiral separations in capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers.
34890854	5	54	theme	cyclodextrin	1361:1372	arg1	manufacturers					1374:1386	cyclodextrin manufacturers	1361:1386	cyclodextrin manufacturers	1361:1386	The study indicated the importance of the type and composition of derivatized CDs on chiral separations in capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers.
34890854	4	55	theme	low	912:914	arg1	content					935:941	low 2,6-DM-β-CD isomer content	912:941	low 2,6-DM-β-CD isomer content	912:941	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	56	theme	d-glucopyranose	1016:1030	arg1	moieties					1032:1039	d-glucopyranose moieties	1016:1039	d-glucopyranose moieties	1016:1039	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	3	57	attach	presence	762:769	arg2	content					793:799	a high 2,6-DM-β-CD content	774:799	a high 2,6-DM-β-CD content	774:799	HPLC and NMR analyses confirmed that the presence of a high 2,6-DM-β-CD content in the CDs enables to achieve the migration order R,R,R,R-enantiomer > daclatasvir.
34890854	3	57	attach	presence	762:769	arg1	CDs					808:810	the CDs	804:810	the CDs	804:810	HPLC and NMR analyses confirmed that the presence of a high 2,6-DM-β-CD content in the CDs enables to achieve the migration order R,R,R,R-enantiomer > daclatasvir.
34890854	0	58	theme	capillary	188:196	arg1	enantioseparation					214:230	the capillary electrophoresis enantioseparation	184:230	the capillary electrophoresis enantioseparation of daclatasvir	184:245	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.
34890854	0	59	theme	liquid	74:79	arg1	chromatography					81:94	high-performance liquid chromatography	57:94	high-performance liquid chromatography	57:94	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.
34890854	1	60	theme	various	352:358	arg1	-β-CD					433:437	the single isomer heptakis(2,6-di-O-methyl)-β-CD	390:437	the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD)	390:451	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	1	60	theme	various	352:358	arg1	selectors					463:471	chiral selectors	456:471	chiral selectors in an acidic background electrolyte	456:507	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	1	60	theme	various	352:358	arg1	β-CDs					380:384	various randomly methylated β-CDs	352:384	various randomly methylated β-CDs	352:384	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	1	61	theme	methylated	369:378	arg1	-β-CD					433:437	the single isomer heptakis(2,6-di-O-methyl)-β-CD	390:437	the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD)	390:451	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	1	61	theme	methylated	369:378	arg1	selectors					463:471	chiral selectors	456:471	chiral selectors in an acidic background electrolyte	456:507	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	1	61	theme	methylated	369:378	arg1	β-CDs					380:384	various randomly methylated β-CDs	352:384	various randomly methylated β-CDs	352:384	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	3	62	theme	NMR	730:732	arg1	analyses					734:741	HPLC and NMR analyses	721:741	HPLC and NMR analyses	721:741	HPLC and NMR analyses confirmed that the presence of a high 2,6-DM-β-CD content in the CDs enables to achieve the migration order R,R,R,R-enantiomer > daclatasvir.
34890854	6	63	dep	composition	1463:1473	arg1	the					1459:1461	the	1459:1461	the	1459:1461	Moreover, the observed migration order could be rationalized based on the composition and substitution pattern of the CDs.
34890854	4	64	with	products	898:905	arg1	content					935:941	low 2,6-DM-β-CD isomer content	912:941	low 2,6-DM-β-CD isomer content	912:941	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	65	theme	isomer	928:933	arg1	content					935:941	low 2,6-DM-β-CD isomer content	912:941	low 2,6-DM-β-CD isomer content	912:941	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	0	66	from	effect	144:149	arg1	enantioseparation					214:230	the capillary electrophoresis enantioseparation	184:230	the capillary electrophoresis enantioseparation of daclatasvir	184:245	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.
34890854	3	67	from	presence	762:769	arg1	CDs					808:810	the CDs	804:810	the CDs	804:810	HPLC and NMR analyses confirmed that the presence of a high 2,6-DM-β-CD content in the CDs enables to achieve the migration order R,R,R,R-enantiomer > daclatasvir.
34890854	0	68	theme	electrophoresis	198:212	arg1	enantioseparation					214:230	the capillary electrophoresis enantioseparation	184:230	the capillary electrophoresis enantioseparation of daclatasvir	184:245	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.
34890854	3	69	theme	HPLC	721:724	arg1	analyses					734:741	HPLC and NMR analyses	721:741	HPLC and NMR analyses	721:741	HPLC and NMR analyses confirmed that the presence of a high 2,6-DM-β-CD content in the CDs enables to achieve the migration order R,R,R,R-enantiomer > daclatasvir.
34890854	5	70	theme	proper	1334:1339	arg1	control					1349:1355	proper quality control	1334:1355	proper quality control	1334:1355	The study indicated the importance of the type and composition of derivatized CDs on chiral separations in capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers.
34890854	6	71	theme	CDs	1507:1509	arg1	composition					1463:1473	composition	1463:1473	composition	1463:1473	Moreover, the observed migration order could be rationalized based on the composition and substitution pattern of the CDs.
34890854	6	71	theme	CDs	1507:1509	arg1	pattern					1492:1498	substitution pattern	1479:1498	substitution pattern	1479:1498	Moreover, the observed migration order could be rationalized based on the composition and substitution pattern of the CDs.
34890854	2	72	with	2,6-DM-β-CD	600:610	arg1	composition					655:665	different composition	645:665	different composition	645:665	Opposite enantiomer migration order was observed for randomly substituted CDs compared to 2,6-DM-β-CD as well as methylated β-CDs with different composition according to the specifications of the manufacturers.
34890854	1	73	theme	R	286:286	arg1	separation					252:261	The separation	248:261	The separation of daclatasvir and its R,R,R,R-enantiomer	248:303	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	0	74	theme	nuclear	100:106	arg1	resonance					117:125	nuclear magnetic resonance	100:125	nuclear magnetic resonance spectroscopy	100:138	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.
34890854	1	75	theme	chiral	456:461	arg1	-β-CD					433:437	the single isomer heptakis(2,6-di-O-methyl)-β-CD	390:437	the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD)	390:451	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	1	75	theme	chiral	456:461	arg1	selectors					463:471	chiral selectors	456:471	chiral selectors in an acidic background electrolyte	456:507	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	1	75	theme	chiral	456:461	arg1	β-CDs					380:384	various randomly methylated β-CDs	352:384	various randomly methylated β-CDs	352:384	The separation of daclatasvir and its R,R,R,R-enantiomer was studied by capillary electrophoresis using various randomly methylated β-CDs and the single isomer heptakis(2,6-di-O-methyl)-β-CD (2,6-DM-β-CD) as chiral selectors in an acidic background electrolyte.
34890854	5	76	theme	quality	1341:1347	arg1	control					1349:1355	proper quality control	1334:1355	proper quality control	1334:1355	The study indicated the importance of the type and composition of derivatized CDs on chiral separations in capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers.
34890854	6	77	theme	substitution	1479:1490	arg1	pattern					1492:1498	substitution pattern	1479:1498	substitution pattern	1479:1498	Moreover, the observed migration order could be rationalized based on the composition and substitution pattern of the CDs.
34890854	3	78	theme	>	870:870	arg1	daclatasvir					872:882	R-enantiomer > daclatasvir	857:882	R-enantiomer > daclatasvir	857:882	HPLC and NMR analyses confirmed that the presence of a high 2,6-DM-β-CD content in the CDs enables to achieve the migration order R,R,R,R-enantiomer > daclatasvir.
34890854	3	78	theme	>	870:870	arg1	R					851:851	R	851:851	R	851:851	HPLC and NMR analyses confirmed that the presence of a high 2,6-DM-β-CD content in the CDs enables to achieve the migration order R,R,R,R-enantiomer > daclatasvir.
34890854	3	79	theme	migration	835:843	arg1	order					845:849	the migration order R,R,R,R-enantiomer > daclatasvir	831:882	the migration order R,R,R,R-enantiomer > daclatasvir	831:882	HPLC and NMR analyses confirmed that the presence of a high 2,6-DM-β-CD content in the CDs enables to achieve the migration order R,R,R,R-enantiomer > daclatasvir.
34890854	0	80	from	methyl-β-cyclodextrins	31:52	arg1	enantioseparation					214:230	the capillary electrophoresis enantioseparation	184:230	the capillary electrophoresis enantioseparation of daclatasvir	184:245	Structural characterization of methyl-β-cyclodextrins by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy and effect of their isomeric composition on the capillary electrophoresis enantioseparation of daclatasvir.
34890854	5	81	theme	control	1349:1355	arg1	importance					1320:1329	the importance	1316:1329	capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers	1279:1386	The study indicated the importance of the type and composition of derivatized CDs on chiral separations in capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers.
34890854	5	81	theme	control	1349:1355	arg1	electrophoresis					1289:1303	capillary electrophoresis	1279:1303	capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers	1279:1386	The study indicated the importance of the type and composition of derivatized CDs on chiral separations in capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers.
34890854	4	82	theme	large	968:972	arg1	amount					974:979	a large amount	966:979	a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6,	966:1086	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	4	82	theme	large	968:972	arg1	isomers					998:1004	methylated CD isomers	984:1004	methylated CD isomers	984:1004	In contrast, products with low 2,6-DM-β-CD isomer content and/or the presence of a large amount of methylated CD isomers, in which d-glucopyranose moieties are not substituted in either position 2 or 6, displayed the opposite enantiomer migration order daclatasvir > R,R,R,R-enantiomer.
34890854	5	83	theme	composition	1223:1233	arg1	importance					1196:1205	the importance	1192:1205	the importance of the type and composition of derivatized CDs on chiral separations in capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers	1192:1386	The study indicated the importance of the type and composition of derivatized CDs on chiral separations in capillary electrophoresis as well as the importance of proper quality control for cyclodextrin manufacturers.
32070555	0	0	theme	biomechanical	97:109	arg1	hotspots					111:118	"biomechanical hotspots	96:118	"biomechanical hotspots"	96:119	Isolation and characterization of microfibrillated cellulose and nanofibrillated cellulose with "biomechanical hotspots".
32070555	6	1	theme	nanocellulose-xyloglucan-pectin	991:1021	arg1	configuration					1023:1035	nanocellulose-xyloglucan-pectin configuration	991:1035	nanocellulose-xyloglucan-pectin configuration	991:1035	Presence of calcium elements in two types of NFCs provided important evidence of exposure of nanocellulose-xyloglucan-pectin configuration responsible for coil-shaped fine-spots resembling "biomechanical hot spots".
32070555	3	2	theme	carbonaceous	529:540	arg1	structures					542:551	carbonaceous structures	529:551	carbonaceous structures	529:551	WA-XRD and FESEM analysis of MFCs further revealed presence of highly crystalline, fine-shaped materials that resemble carbonaceous structures.
32070555	5	3	theme	NFCs	797:800	arg1	STEM					769:772	STEM	769:772	STEM	769:772	Morphology even got clearer when STEM and EDX mapping of the NFCs assured that observed fine-spots are integral and possibly due to certain biosynthetic process.
32070555	5	3	theme	NFCs	797:800	arg1	mapping					782:788	EDX mapping	778:788	EDX mapping	778:788	Morphology even got clearer when STEM and EDX mapping of the NFCs assured that observed fine-spots are integral and possibly due to certain biosynthetic process.
32070555	4	4	theme	HR-TEM	554:559	arg1	analysis					561:568	HR-TEM analysis	554:568	HR-TEM analysis	554:568	HR-TEM analysis further exposed morphology of the two nanofibrils possessing a well entangled network (diameter ≤80 nm) comprising coil shaped fine spots of size up to 80 nm-220 nm.
32070555	1	5	theme	nano-fibrillated	275:290	arg1	NFCs					313:316	NFCs	313:316	NFCs	313:316	Waste biomass of two major milk weeds was separately given combination of chemical-mechanical treatments in order to expose natural cellulosic micro and nano-fibrillated structures (MFCs and NFCs).
32070555	1	5	theme	nano-fibrillated	275:290	arg1	MFCs					304:307	MFCs	304:307	MFCs	304:307	Waste biomass of two major milk weeds was separately given combination of chemical-mechanical treatments in order to expose natural cellulosic micro and nano-fibrillated structures (MFCs and NFCs).
32070555	1	5	theme	nano-fibrillated	275:290	arg1	structures					292:301	nano-fibrillated structures	275:301	nano-fibrillated structures (MFCs and NFCs)	275:317	Waste biomass of two major milk weeds was separately given combination of chemical-mechanical treatments in order to expose natural cellulosic micro and nano-fibrillated structures (MFCs and NFCs).
32070555	4	6	theme	entangled	638:646	arg1	network					648:654	a well entangled network	631:654	a well entangled network (diameter ≤80 nm) comprising coil shaped fine spots of size up to 80 nm-220 nm	631:733	HR-TEM analysis further exposed morphology of the two nanofibrils possessing a well entangled network (diameter ≤80 nm) comprising coil shaped fine spots of size up to 80 nm-220 nm.
32070555	4	6	theme	entangled	638:646	arg1	≤80 nm					666:671	diameter ≤80 nm	657:671	diameter ≤80 nm	657:671	HR-TEM analysis further exposed morphology of the two nanofibrils possessing a well entangled network (diameter ≤80 nm) comprising coil shaped fine spots of size up to 80 nm-220 nm.
32070555	3	7	theme	materials	505:513	arg1	presence					461:468	presence	461:468	presence of highly crystalline, fine-shaped materials that resemble carbonaceous structures	461:551	WA-XRD and FESEM analysis of MFCs further revealed presence of highly crystalline, fine-shaped materials that resemble carbonaceous structures.
32070555	3	8	theme	MFCs	439:442	arg1	analysis					427:434	WA-XRD and FESEM analysis	410:434	WA-XRD and FESEM analysis of MFCs	410:442	WA-XRD and FESEM analysis of MFCs further revealed presence of highly crystalline, fine-shaped materials that resemble carbonaceous structures.
32070555	5	9	theme	EDX	778:780	arg1	mapping					782:788	EDX mapping	778:788	EDX mapping	778:788	Morphology even got clearer when STEM and EDX mapping of the NFCs assured that observed fine-spots are integral and possibly due to certain biosynthetic process.
32070555	6	10	theme	elements	918:925	arg1	Presence					898:905	Presence	898:905	Presence of calcium elements in two types of NFCs	898:946	Presence of calcium elements in two types of NFCs provided important evidence of exposure of nanocellulose-xyloglucan-pectin configuration responsible for coil-shaped fine-spots resembling "biomechanical hot spots".
32070555	6	11	theme	hot	1102:1104	arg1	spots					1106:1110	"biomechanical hot spots	1087:1110	"biomechanical hot spots"	1087:1111	Presence of calcium elements in two types of NFCs provided important evidence of exposure of nanocellulose-xyloglucan-pectin configuration responsible for coil-shaped fine-spots resembling "biomechanical hot spots".
32070555	4	12	theme	fine	697:700	arg1	spots					702:706	coil shaped fine spots	685:706	coil shaped fine spots of size up to 80 nm-220 nm	685:733	HR-TEM analysis further exposed morphology of the two nanofibrils possessing a well entangled network (diameter ≤80 nm) comprising coil shaped fine spots of size up to 80 nm-220 nm.
32070555	6	13	theme	calcium	910:916	arg1	elements					918:925	calcium elements	910:925	calcium elements	910:925	Presence of calcium elements in two types of NFCs provided important evidence of exposure of nanocellulose-xyloglucan-pectin configuration responsible for coil-shaped fine-spots resembling "biomechanical hot spots".
32070555	5	14	dep	assured	802:808	arg1	Morphology					736:745	Morphology	736:745	Morphology	736:745	Morphology even got clearer when STEM and EDX mapping of the NFCs assured that observed fine-spots are integral and possibly due to certain biosynthetic process.
32070555	5	14	dep	assured	802:808	arg1	clearer					756:762	clearer	756:762	clearer	756:762	Morphology even got clearer when STEM and EDX mapping of the NFCs assured that observed fine-spots are integral and possibly due to certain biosynthetic process.
32070555	2	15	theme	FTIR	338:341	arg1	analysis					343:350	FTIR analysis	338:350	FTIR analysis	338:350	Compositional and FTIR analysis were initially used to confirm the removal of impurities.
32070555	2	16	theme	impurities	398:407	arg1	removal					387:393	the removal	383:393	the removal of impurities	383:407	Compositional and FTIR analysis were initially used to confirm the removal of impurities.
32070555	3	17	theme	WA-XRD	410:415	arg1	analysis					427:434	WA-XRD and FESEM analysis	410:434	WA-XRD and FESEM analysis of MFCs	410:442	WA-XRD and FESEM analysis of MFCs further revealed presence of highly crystalline, fine-shaped materials that resemble carbonaceous structures.
32070555	6	18	theme	responsible	1037:1047	arg1	evidence					967:974	important evidence	957:974	important evidence of exposure of nanocellulose-xyloglucan-pectin configuration responsible for coil-shaped fine-spots resembling "biomechanical hot spots"	957:1111	Presence of calcium elements in two types of NFCs provided important evidence of exposure of nanocellulose-xyloglucan-pectin configuration responsible for coil-shaped fine-spots resembling "biomechanical hot spots".
32070555	1	19	theme	chemical-mechanical	196:214	arg1	treatments					216:225	chemical-mechanical treatments	196:225	chemical-mechanical treatments	196:225	Waste biomass of two major milk weeds was separately given combination of chemical-mechanical treatments in order to expose natural cellulosic micro and nano-fibrillated structures (MFCs and NFCs).
32070555	6	20	theme	configuration	1023:1035	arg1	exposure					979:986	exposure	979:986	exposure of nanocellulose-xyloglucan-pectin configuration	979:1035	Presence of calcium elements in two types of NFCs provided important evidence of exposure of nanocellulose-xyloglucan-pectin configuration responsible for coil-shaped fine-spots resembling "biomechanical hot spots".
32070555	2	21	used	used	367:370	arg2	analysis					343:350	FTIR analysis	338:350	FTIR analysis	338:350	Compositional and FTIR analysis were initially used to confirm the removal of impurities.
32070555	2	21	used	used	367:370	arg2	Compositional					320:332	Compositional	320:332	Compositional	320:332	Compositional and FTIR analysis were initially used to confirm the removal of impurities.
32070555	5	22	theme	observed	815:822	arg1	fine-spots					824:833	observed fine-spots	815:833	observed fine-spots	815:833	Morphology even got clearer when STEM and EDX mapping of the NFCs assured that observed fine-spots are integral and possibly due to certain biosynthetic process.
32070555	1	23	theme	treatments	216:225	arg1	combination					181:191	combination	181:191	combination of chemical-mechanical treatments	181:225	Waste biomass of two major milk weeds was separately given combination of chemical-mechanical treatments in order to expose natural cellulosic micro and nano-fibrillated structures (MFCs and NFCs).
32070555	6	24	from	Presence	898:905	arg1	types					934:938	two types	930:938	two types of NFCs	930:946	Presence of calcium elements in two types of NFCs provided important evidence of exposure of nanocellulose-xyloglucan-pectin configuration responsible for coil-shaped fine-spots resembling "biomechanical hot spots".
32070555	4	25	theme	nanofibrils	608:618	arg1	morphology					586:595	morphology	586:595	morphology of the two nanofibrils possessing a well entangled network (diameter ≤80 nm) comprising coil shaped fine spots of size up to 80 nm-220 nm	586:733	HR-TEM analysis further exposed morphology of the two nanofibrils possessing a well entangled network (diameter ≤80 nm) comprising coil shaped fine spots of size up to 80 nm-220 nm.
32070555	1	26	theme	Waste	122:126	arg1	biomass					128:134	Waste biomass	122:134	Waste biomass of two major milk weeds	122:158	Waste biomass of two major milk weeds was separately given combination of chemical-mechanical treatments in order to expose natural cellulosic micro and nano-fibrillated structures (MFCs and NFCs).
32070555	6	27	theme	NFCs	943:946	arg1	types					934:938	two types	930:938	two types of NFCs	930:946	Presence of calcium elements in two types of NFCs provided important evidence of exposure of nanocellulose-xyloglucan-pectin configuration responsible for coil-shaped fine-spots resembling "biomechanical hot spots".
32070555	0	28	theme	cellulose	51:59	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Isolation and characterization of microfibrillated cellulose and nanofibrillated cellulose with "biomechanical hotspots".
32070555	0	28	theme	cellulose	51:59	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and characterization of microfibrillated cellulose and nanofibrillated cellulose with "biomechanical hotspots".
32070555	4	29	theme	size	711:714	arg1	spots					702:706	coil shaped fine spots	685:706	coil shaped fine spots of size up to 80 nm-220 nm	685:733	HR-TEM analysis further exposed morphology of the two nanofibrils possessing a well entangled network (diameter ≤80 nm) comprising coil shaped fine spots of size up to 80 nm-220 nm.
32070555	5	30	theme	certain	868:874	arg1	process					889:895	certain biosynthetic process	868:895	certain biosynthetic process	868:895	Morphology even got clearer when STEM and EDX mapping of the NFCs assured that observed fine-spots are integral and possibly due to certain biosynthetic process.
32070555	4	31	theme	shaped	690:695	arg1	spots					702:706	coil shaped fine spots	685:706	coil shaped fine spots of size up to 80 nm-220 nm	685:733	HR-TEM analysis further exposed morphology of the two nanofibrils possessing a well entangled network (diameter ≤80 nm) comprising coil shaped fine spots of size up to 80 nm-220 nm.
32070555	0	32	theme	microfibrillated	34:49	arg1	cellulose					51:59	microfibrillated cellulose	34:59	microfibrillated cellulose	34:59	Isolation and characterization of microfibrillated cellulose and nanofibrillated cellulose with "biomechanical hotspots".
32070555	6	33	theme	exposure	979:986	arg1	evidence					967:974	important evidence	957:974	important evidence of exposure of nanocellulose-xyloglucan-pectin configuration responsible for coil-shaped fine-spots resembling "biomechanical hot spots"	957:1111	Presence of calcium elements in two types of NFCs provided important evidence of exposure of nanocellulose-xyloglucan-pectin configuration responsible for coil-shaped fine-spots resembling "biomechanical hot spots".
32070555	6	34	theme	biomechanical	1088:1100	arg1	spots					1106:1110	"biomechanical hot spots	1087:1110	"biomechanical hot spots"	1087:1111	Presence of calcium elements in two types of NFCs provided important evidence of exposure of nanocellulose-xyloglucan-pectin configuration responsible for coil-shaped fine-spots resembling "biomechanical hot spots".
32070555	4	35	theme	coil	685:688	arg1	spots					702:706	coil shaped fine spots	685:706	coil shaped fine spots of size up to 80 nm-220 nm	685:733	HR-TEM analysis further exposed morphology of the two nanofibrils possessing a well entangled network (diameter ≤80 nm) comprising coil shaped fine spots of size up to 80 nm-220 nm.
32070555	0	36	theme	nanofibrillated	65:79	arg1	cellulose					81:89	nanofibrillated cellulose	65:89	nanofibrillated cellulose	65:89	Isolation and characterization of microfibrillated cellulose and nanofibrillated cellulose with "biomechanical hotspots".
32070555	4	37	contain	possessing	620:629	arg2	≤80 nm					666:671	diameter ≤80 nm	657:671	diameter ≤80 nm	657:671	HR-TEM analysis further exposed morphology of the two nanofibrils possessing a well entangled network (diameter ≤80 nm) comprising coil shaped fine spots of size up to 80 nm-220 nm.
32070555	4	37	contain	possessing	620:629	arg2	network					648:654	a well entangled network	631:654	a well entangled network (diameter ≤80 nm) comprising coil shaped fine spots of size up to 80 nm-220 nm	631:733	HR-TEM analysis further exposed morphology of the two nanofibrils possessing a well entangled network (diameter ≤80 nm) comprising coil shaped fine spots of size up to 80 nm-220 nm.
32070555	4	37	contain	possessing	620:629	arg1	nanofibrils					608:618	the two nanofibrils	600:618	the two nanofibrils possessing a well entangled network (diameter ≤80 nm) comprising coil shaped fine spots of size up to 80 nm-220 nm	600:733	HR-TEM analysis further exposed morphology of the two nanofibrils possessing a well entangled network (diameter ≤80 nm) comprising coil shaped fine spots of size up to 80 nm-220 nm.
32070555	0	38	with	characterization	14:29	arg1	hotspots					111:118	"biomechanical hotspots	96:118	"biomechanical hotspots"	96:119	Isolation and characterization of microfibrillated cellulose and nanofibrillated cellulose with "biomechanical hotspots".
32070555	3	39	theme	FESEM	421:425	arg1	analysis					427:434	WA-XRD and FESEM analysis	410:434	WA-XRD and FESEM analysis of MFCs	410:442	WA-XRD and FESEM analysis of MFCs further revealed presence of highly crystalline, fine-shaped materials that resemble carbonaceous structures.
32070555	1	40	dep	structures	292:301	arg1	NFCs					313:316	NFCs	313:316	NFCs	313:316	Waste biomass of two major milk weeds was separately given combination of chemical-mechanical treatments in order to expose natural cellulosic micro and nano-fibrillated structures (MFCs and NFCs).
32070555	1	40	dep	structures	292:301	arg1	MFCs					304:307	MFCs	304:307	MFCs	304:307	Waste biomass of two major milk weeds was separately given combination of chemical-mechanical treatments in order to expose natural cellulosic micro and nano-fibrillated structures (MFCs and NFCs).
32070555	1	40	dep	structures	292:301	arg1	structures					292:301	nano-fibrillated structures	275:301	nano-fibrillated structures (MFCs and NFCs)	275:317	Waste biomass of two major milk weeds was separately given combination of chemical-mechanical treatments in order to expose natural cellulosic micro and nano-fibrillated structures (MFCs and NFCs).
32070555	6	41	theme	important	957:965	arg1	evidence					967:974	important evidence	957:974	important evidence of exposure of nanocellulose-xyloglucan-pectin configuration responsible for coil-shaped fine-spots resembling "biomechanical hot spots"	957:1111	Presence of calcium elements in two types of NFCs provided important evidence of exposure of nanocellulose-xyloglucan-pectin configuration responsible for coil-shaped fine-spots resembling "biomechanical hot spots".
32070555	0	42	with	Isolation	0:8	arg1	hotspots					111:118	"biomechanical hotspots	96:118	"biomechanical hotspots"	96:119	Isolation and characterization of microfibrillated cellulose and nanofibrillated cellulose with "biomechanical hotspots".
32070555	6	43	theme	coil-shaped	1053:1063	arg1	fine-spots					1065:1074	coil-shaped fine-spots	1053:1074	coil-shaped fine-spots resembling "biomechanical hot spots"	1053:1111	Presence of calcium elements in two types of NFCs provided important evidence of exposure of nanocellulose-xyloglucan-pectin configuration responsible for coil-shaped fine-spots resembling "biomechanical hot spots".
32070555	1	44	theme	major	143:147	arg1	weeds					154:158	two major milk weeds	139:158	two major milk weeds	139:158	Waste biomass of two major milk weeds was separately given combination of chemical-mechanical treatments in order to expose natural cellulosic micro and nano-fibrillated structures (MFCs and NFCs).
32070555	1	45	theme	natural	246:252	arg1	micro					265:269	natural cellulosic micro	246:269	natural cellulosic micro	246:269	Waste biomass of two major milk weeds was separately given combination of chemical-mechanical treatments in order to expose natural cellulosic micro and nano-fibrillated structures (MFCs and NFCs).
32070555	5	46	theme	biosynthetic	876:887	arg1	process					889:895	certain biosynthetic process	868:895	certain biosynthetic process	868:895	Morphology even got clearer when STEM and EDX mapping of the NFCs assured that observed fine-spots are integral and possibly due to certain biosynthetic process.
32070555	3	47	dep	crystalline	480:490	arg1	fine-shaped					493:503	fine-shaped	493:503	fine-shaped	493:503	WA-XRD and FESEM analysis of MFCs further revealed presence of highly crystalline, fine-shaped materials that resemble carbonaceous structures.
32070555	3	48	theme	crystalline	480:490	arg1	materials					505:513	highly crystalline, fine-shaped materials	473:513	highly crystalline, fine-shaped materials that resemble carbonaceous structures	473:551	WA-XRD and FESEM analysis of MFCs further revealed presence of highly crystalline, fine-shaped materials that resemble carbonaceous structures.
32070555	1	49	theme	milk	149:152	arg1	weeds					154:158	two major milk weeds	139:158	two major milk weeds	139:158	Waste biomass of two major milk weeds was separately given combination of chemical-mechanical treatments in order to expose natural cellulosic micro and nano-fibrillated structures (MFCs and NFCs).
32070555	1	50	theme	cellulosic	254:263	arg1	micro					265:269	natural cellulosic micro	246:269	natural cellulosic micro	246:269	Waste biomass of two major milk weeds was separately given combination of chemical-mechanical treatments in order to expose natural cellulosic micro and nano-fibrillated structures (MFCs and NFCs).
32070555	0	51	theme	cellulose	81:89	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Isolation and characterization of microfibrillated cellulose and nanofibrillated cellulose with "biomechanical hotspots".
32070555	0	51	theme	cellulose	81:89	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and characterization of microfibrillated cellulose and nanofibrillated cellulose with "biomechanical hotspots".
32070555	1	52	theme	weeds	154:158	arg1	biomass					128:134	Waste biomass	122:134	Waste biomass of two major milk weeds	122:158	Waste biomass of two major milk weeds was separately given combination of chemical-mechanical treatments in order to expose natural cellulosic micro and nano-fibrillated structures (MFCs and NFCs).
32070555	4	53	theme	diameter	657:664	arg1	network					648:654	a well entangled network	631:654	a well entangled network (diameter ≤80 nm) comprising coil shaped fine spots of size up to 80 nm-220 nm	631:733	HR-TEM analysis further exposed morphology of the two nanofibrils possessing a well entangled network (diameter ≤80 nm) comprising coil shaped fine spots of size up to 80 nm-220 nm.
32070555	4	53	theme	diameter	657:664	arg1	≤80 nm					666:671	diameter ≤80 nm	657:671	diameter ≤80 nm	657:671	HR-TEM analysis further exposed morphology of the two nanofibrils possessing a well entangled network (diameter ≤80 nm) comprising coil shaped fine spots of size up to 80 nm-220 nm.
32814582	11	0	theme	gut	2720:2722	arg1	features					2735:2742	relevant gut microbiota features	2711:2742	relevant gut microbiota features	2711:2742	This provides a basis to personalize the use of dietary fiber in nutritional application and to stratify human populations by relevant gut microbiota features to account for the inconsistent health effects in human intervention studies.
32814582	0	1	attach	linked	106:111	arg1	increase					143:150	an individualized temporal increase	116:150	an individualized temporal increase in fecal propionate	116:170	Gut microbiota modulation with long-chain corn bran arabinoxylan in adults with overweight and obesity is linked to an individualized temporal increase in fecal propionate.
32814582	0	1	attach	linked	106:111	arg2	modulation					15:24	Gut microbiota modulation	0:24	Gut microbiota modulation with long-chain corn bran arabinoxylan in adults with overweight and obesity	0:101	Gut microbiota modulation with long-chain corn bran arabinoxylan in adults with overweight and obesity is linked to an individualized temporal increase in fecal propionate.
32814582	7	2	attach	derived	1761:1767	arg2	PCs					1756:1758	PCs	1756:1758	PCs	1756:1758	Principal components (PCs) derived from community data were better predictors in MLR models as compared to single taxa, indicating that arabinoxylan fermentation is the result of multi-species interactions within microbiomes.
32814582	7	2	attach	derived	1761:1767	arg1	data					1784:1787	community data	1774:1787	community data	1774:1787	Principal components (PCs) derived from community data were better predictors in MLR models as compared to single taxa, indicating that arabinoxylan fermentation is the result of multi-species interactions within microbiomes.
32814582	7	2	attach	derived	1761:1767	arg2	components					1744:1753	Principal components	1734:1753	Principal components (PCs) derived from community data	1734:1787	Principal components (PCs) derived from community data were better predictors in MLR models as compared to single taxa, indicating that arabinoxylan fermentation is the result of multi-species interactions within microbiomes.
32814582	3	3	theme	complex	721:727	arg1	n					743:743	n = 15	743:748	n = 15	743:748	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	3	3	theme	complex	721:727	arg1	arabinoxylan					729:740	complex arabinoxylan	721:740	complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition	721:844	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	1	4	theme	inter-individual	252:267	arg1	differences					269:279	inter-individual differences	252:279	inter-individual differences in the gut microbiota's ability to ferment these substrates into beneficial metabolites	252:367	BACKGROUND Variability in the health effects of dietary fiber might arise from inter-individual differences in the gut microbiota's ability to ferment these substrates into beneficial metabolites.
32814582	8	5	dep	CONCLUSION	1960:1969	arg1	showed					1982:1987	showed	1982:1987	showed that long-chain arabinoxylan modulates both microbiota composition and the output of health-relevant SCFAs, providing information for a more targeted application of this fiber	1982:2163	CONCLUSION This study showed that long-chain arabinoxylan modulates both microbiota composition and the output of health-relevant SCFAs, providing information for a more targeted application of this fiber.
32814582	3	6	theme	ecological	998:1007	arg1	framework					1009:1017	an ecological framework	995:1017	an ecological framework	995:1017	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	0	7	with	adults	68:73	arg1	obesity					95:101	obesity	95:101	obesity	95:101	Gut microbiota modulation with long-chain corn bran arabinoxylan in adults with overweight and obesity is linked to an individualized temporal increase in fecal propionate.
32814582	0	7	with	adults	68:73	arg1	overweight					80:89	overweight	80:89	overweight	80:89	Gut microbiota modulation with long-chain corn bran arabinoxylan in adults with overweight and obesity is linked to an individualized temporal increase in fecal propionate.
32814582	6	8	theme	≤	1533:1533	arg1	PERMANOVA					1542:1550	PERMANOVA	1542:1550	PERMANOVA	1542:1550	The two groups showed differences in compositional shifts of the microbiota (p ≤ 0.025, PERMANOVA), and multiple linear regression (MLR) analyses revealed that the propionate response was predictable through shifts and, to a lesser degree, baseline composition of the microbiota.
32814582	6	8	theme	≤	1533:1533	arg1	p					1531:1531	p ≤ 0.025	1531:1539	p ≤ 0.025	1531:1539	The two groups showed differences in compositional shifts of the microbiota (p ≤ 0.025, PERMANOVA), and multiple linear regression (MLR) analyses revealed that the propionate response was predictable through shifts and, to a lesser degree, baseline composition of the microbiota.
32814582	10	9	theme	community	2503:2511	arg1	features					2467:2474	features	2467:2474	features of the wider gut microbial community for the prediction of metabolic outcomes of dietary fiber fermentation	2467:2582	These findings constitute a proof-of-concept for the merit of an ecological framework that considers features of the wider gut microbial community for the prediction of metabolic outcomes of dietary fiber fermentation.
32814582	3	10	theme	=	745:745	arg1	n					743:743	n = 15	743:748	n = 15	743:748	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	3	10	theme	=	745:745	arg1	arabinoxylan					729:740	complex arabinoxylan	721:740	complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition	721:844	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	1	11	theme	BACKGROUND	173:182	arg1	Variability					184:194	BACKGROUND Variability	173:194	BACKGROUND Variability in the health effects of dietary fiber	173:233	BACKGROUND Variability in the health effects of dietary fiber might arise from inter-individual differences in the gut microbiota's ability to ferment these substrates into beneficial metabolites.
32814582	6	12	dep	microbiota	1519:1528	arg1	PERMANOVA					1542:1550	PERMANOVA	1542:1550	PERMANOVA	1542:1550	The two groups showed differences in compositional shifts of the microbiota (p ≤ 0.025, PERMANOVA), and multiple linear regression (MLR) analyses revealed that the propionate response was predictable through shifts and, to a lesser degree, baseline composition of the microbiota.
32814582	6	12	dep	microbiota	1519:1528	arg1	p					1531:1531	p ≤ 0.025	1531:1539	p ≤ 0.025	1531:1539	The two groups showed differences in compositional shifts of the microbiota (p ≤ 0.025, PERMANOVA), and multiple linear regression (MLR) analyses revealed that the propionate response was predictable through shifts and, to a lesser degree, baseline composition of the microbiota.
32814582	10	13	theme	gut	2489:2491	arg1	community					2503:2511	the wider gut microbial community	2479:2511	the wider gut microbial community for the prediction of metabolic outcomes of dietary fiber fermentation	2479:2582	These findings constitute a proof-of-concept for the merit of an ecological framework that considers features of the wider gut microbial community for the prediction of metabolic outcomes of dietary fiber fermentation.
32814582	5	14	theme	distinct	1396:1403	arg1	groupings					1405:1413	two distinct groupings	1392:1413	two distinct groupings of temporal responses in participants	1392:1451	Arabinoxylan further increased fecal propionate concentrations (p = 0.012, Friedman's test), an effect that showed two distinct groupings of temporal responses in participants.
32814582	3	15	theme	controlled	612:621	arg1	trial					623:627	exploratory randomized controlled trial	589:627	exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control)	589:958	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	3	15	theme	controlled	612:621	arg1	two-arm					580:586	a parallel two-arm	569:586	a parallel two-arm	569:586	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	4	16	theme	community	1087:1095	arg1	composition					1097:1107	fecal bacterial community composition	1071:1107	fecal bacterial community composition	1071:1107	RESULTS Arabinoxylan resulted in a global shift in fecal bacterial community composition, reduced α-diversity, and the promotion of specific taxa, including operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri.
32814582	3	17	theme	high	754:757	arg1	doses					775:779	high supplementation doses	754:779	high supplementation doses (female: 25 g/day; male: 35 g/day)	754:814	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	3	18	theme	exploratory	589:599	arg1	trial					623:627	exploratory randomized controlled trial	589:627	exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control)	589:958	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	3	18	theme	exploratory	589:599	arg1	two-arm					580:586	a parallel two-arm	569:586	a parallel two-arm	569:586	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	1	19	theme	health	203:208	arg1	effects					210:216	the health effects	199:216	the health effects of dietary fiber	199:233	BACKGROUND Variability in the health effects of dietary fiber might arise from inter-individual differences in the gut microbiota's ability to ferment these substrates into beneficial metabolites.
32814582	11	20	theme	health	2776:2781	arg1	effects					2783:2789	the inconsistent health effects	2759:2789	the inconsistent health effects in human intervention studies	2759:2819	This provides a basis to personalize the use of dietary fiber in nutritional application and to stratify human populations by relevant gut microbiota features to account for the inconsistent health effects in human intervention studies.
32814582	3	21	from	arabinoxylan	729:740	arg1	composition					834:844	gut microbiota composition	819:844	gut microbiota composition	819:844	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	9	22	theme	propionate	2179:2188	arg1	production					2190:2199	propionate production	2179:2199	propionate production	2179:2199	Variation in propionate production was linked to both compositional shifts and baseline composition, with PCs derived from shifts of the global microbial community showing the strongest associations.
32814582	0	23	theme	individualized	119:132	arg1	increase					143:150	an individualized temporal increase	116:150	an individualized temporal increase in fecal propionate	116:170	Gut microbiota modulation with long-chain corn bran arabinoxylan in adults with overweight and obesity is linked to an individualized temporal increase in fecal propionate.
32814582	6	24	theme	linear	1567:1572	arg1	MLR					1586:1588	MLR	1586:1588	MLR	1586:1588	The two groups showed differences in compositional shifts of the microbiota (p ≤ 0.025, PERMANOVA), and multiple linear regression (MLR) analyses revealed that the propionate response was predictable through shifts and, to a lesser degree, baseline composition of the microbiota.
32814582	6	24	theme	linear	1567:1572	arg1	regression					1574:1583	multiple linear regression	1558:1583	multiple linear regression (MLR) analyses	1558:1598	The two groups showed differences in compositional shifts of the microbiota (p ≤ 0.025, PERMANOVA), and multiple linear regression (MLR) analyses revealed that the propionate response was predictable through shifts and, to a lesser degree, baseline composition of the microbiota.
32814582	7	25	theme	multi-species	1913:1925	arg1	interactions					1927:1938	multi-species interactions	1913:1938	multi-species interactions within microbiomes	1913:1957	Principal components (PCs) derived from community data were better predictors in MLR models as compared to single taxa, indicating that arabinoxylan fermentation is the result of multi-species interactions within microbiomes.
32814582	3	26	from	trial	623:627	arg1	adults					635:640	31 adults	632:640	31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control)	632:958	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	10	27	theme	dietary	2557:2563	arg1	fermentation					2571:2582	dietary fiber fermentation	2557:2582	dietary fiber fermentation	2557:2582	These findings constitute a proof-of-concept for the merit of an ecological framework that considers features of the wider gut microbial community for the prediction of metabolic outcomes of dietary fiber fermentation.
32814582	4	28	theme	operational	1177:1187	arg1	units					1199:1203	operational taxonomic units	1177:1203	operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri	1177:1274	RESULTS Arabinoxylan resulted in a global shift in fecal bacterial community composition, reduced α-diversity, and the promotion of specific taxa, including operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri.
32814582	10	29	theme	outcomes	2545:2552	arg1	prediction					2521:2530	the prediction	2517:2530	the prediction of metabolic outcomes of dietary fiber fermentation	2517:2582	These findings constitute a proof-of-concept for the merit of an ecological framework that considers features of the wider gut microbial community for the prediction of metabolic outcomes of dietary fiber fermentation.
32814582	0	30	theme	fecal	155:159	arg1	propionate					161:170	fecal propionate	155:170	fecal propionate	155:170	Gut microbiota modulation with long-chain corn bran arabinoxylan in adults with overweight and obesity is linked to an individualized temporal increase in fecal propionate.
32814582	3	31	theme	class-I	662:668	arg1	obesity					670:676	class-I obesity	662:676	class-I obesity	662:676	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	1	32	from	Variability	184:194	arg1	effects					210:216	the health effects	199:216	the health effects of dietary fiber	199:233	BACKGROUND Variability in the health effects of dietary fiber might arise from inter-individual differences in the gut microbiota's ability to ferment these substrates into beneficial metabolites.
32814582	4	33	from	shift	1062:1066	arg1	promotion					1139:1147	the promotion	1135:1147	the promotion of specific taxa, including operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri	1135:1274	RESULTS Arabinoxylan resulted in a global shift in fecal bacterial community composition, reduced α-diversity, and the promotion of specific taxa, including operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri.
32814582	4	33	from	shift	1062:1066	arg1	composition					1097:1107	fecal bacterial community composition	1071:1107	fecal bacterial community composition	1071:1107	RESULTS Arabinoxylan resulted in a global shift in fecal bacterial community composition, reduced α-diversity, and the promotion of specific taxa, including operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri.
32814582	4	33	from	shift	1062:1066	arg1	α-diversity					1118:1128	reduced α-diversity	1110:1128	reduced α-diversity	1110:1128	RESULTS Arabinoxylan resulted in a global shift in fecal bacterial community composition, reduced α-diversity, and the promotion of specific taxa, including operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri.
32814582	3	34	theme	microbiota	823:832	arg1	composition					834:844	gut microbiota composition	819:844	gut microbiota composition	819:844	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	11	35	theme	human	2690:2694	arg1	populations					2696:2706	human populations	2690:2706	human populations	2690:2706	This provides a basis to personalize the use of dietary fiber in nutritional application and to stratify human populations by relevant gut microbiota features to account for the inconsistent health effects in human intervention studies.
32814582	6	36	theme	propionate	1618:1627	arg1	response					1629:1636	the propionate response	1614:1636	the propionate response	1614:1636	The two groups showed differences in compositional shifts of the microbiota (p ≤ 0.025, PERMANOVA), and multiple linear regression (MLR) analyses revealed that the propionate response was predictable through shifts and, to a lesser degree, baseline composition of the microbiota.
32814582	9	37	theme	compositional	2220:2232	arg1	shifts					2234:2239	compositional shifts	2220:2239	compositional shifts	2220:2239	Variation in propionate production was linked to both compositional shifts and baseline composition, with PCs derived from shifts of the global microbial community showing the strongest associations.
32814582	3	38	theme	production	873:882	arg1	effects					698:704	the effects	694:704	the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production	694:882	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	2	39	theme	complex	513:519	arg1	communities					537:547	complex inter-connected communities	513:547	complex inter-connected communities	513:547	Our understanding of what drives this individuality is vastly incomplete and will require an ecological perspective as microbiomes function as complex inter-connected communities.
32814582	2	39	theme	complex	513:519	arg1	microbiomes					489:499	microbiomes	489:499	microbiomes	489:499	Our understanding of what drives this individuality is vastly incomplete and will require an ecological perspective as microbiomes function as complex inter-connected communities.
32814582	0	40	from	modulation	15:24	arg1	adults					68:73	adults	68:73	adults with overweight and obesity	68:101	Gut microbiota modulation with long-chain corn bran arabinoxylan in adults with overweight and obesity is linked to an individualized temporal increase in fecal propionate.
32814582	4	41	theme	specific	1152:1159	arg1	units					1199:1203	operational taxonomic units	1177:1203	operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri	1177:1274	RESULTS Arabinoxylan resulted in a global shift in fecal bacterial community composition, reduced α-diversity, and the promotion of specific taxa, including operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri.
32814582	4	41	theme	specific	1152:1159	arg1	taxa					1161:1164	specific taxa	1152:1164	specific taxa	1152:1164	RESULTS Arabinoxylan resulted in a global shift in fecal bacterial community composition, reduced α-diversity, and the promotion of specific taxa, including operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri.
32814582	10	42	theme	fermentation	2571:2582	arg1	outcomes					2545:2552	metabolic outcomes	2535:2552	metabolic outcomes of dietary fiber fermentation	2535:2582	These findings constitute a proof-of-concept for the merit of an ecological framework that considers features of the wider gut microbial community for the prediction of metabolic outcomes of dietary fiber fermentation.
32814582	1	43	theme	beneficial	346:355	arg1	metabolites					357:367	beneficial metabolites	346:367	beneficial metabolites	346:367	BACKGROUND Variability in the health effects of dietary fiber might arise from inter-individual differences in the gut microbiota's ability to ferment these substrates into beneficial metabolites.
32814582	3	44	theme	fatty	862:866	arg1	acid					868:871	short-chain fatty acid	850:871	short-chain fatty acid	850:871	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	5	45	theme	responses	1427:1435	arg1	groupings					1405:1413	two distinct groupings	1392:1413	two distinct groupings of temporal responses in participants	1392:1451	Arabinoxylan further increased fecal propionate concentrations (p = 0.012, Friedman's test), an effect that showed two distinct groupings of temporal responses in participants.
32814582	3	46	dep	female	782:787	arg1	male					800:803	male	800:803	female: 25 g/day; male: 35 g/day	782:813	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	3	46	dep	female	782:787	arg1	g/day					809:813	35 g/day	806:813	female: 25 g/day; male: 35 g/day	782:813	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	3	46	dep	female	782:787	arg1	g/day					793:797	25 g/day	790:797	female: 25 g/day; male: 35 g/day	782:813	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	8	47	theme	fiber	2159:2163	arg1	application					2139:2149	a more targeted application	2123:2149	a more targeted application of this fiber	2123:2163	CONCLUSION This study showed that long-chain arabinoxylan modulates both microbiota composition and the output of health-relevant SCFAs, providing information for a more targeted application of this fiber.
32814582	3	48	theme	microcrystalline	899:914	arg1	n					927:927	n = 16	927:932	n = 16	927:932	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	3	48	theme	microcrystalline	899:914	arg1	cellulose					916:924	microcrystalline cellulose	899:924	microcrystalline cellulose (n = 16, non-fermentable control)	899:958	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	6	49	theme	lesser	1679:1684	arg1	degree					1686:1691	a lesser degree	1677:1691	a lesser degree	1677:1691	The two groups showed differences in compositional shifts of the microbiota (p ≤ 0.025, PERMANOVA), and multiple linear regression (MLR) analyses revealed that the propionate response was predictable through shifts and, to a lesser degree, baseline composition of the microbiota.
32814582	5	50	theme	=	1343:1343	arg1	concentrations					1325:1338	fecal propionate concentrations	1308:1338	fecal propionate concentrations (p = 0.012, Friedman's test)	1308:1367	Arabinoxylan further increased fecal propionate concentrations (p = 0.012, Friedman's test), an effect that showed two distinct groupings of temporal responses in participants.
32814582	5	50	theme	=	1343:1343	arg1	0.012					1345:1349	p = 0.012	1341:1349	p = 0.012	1341:1349	Arabinoxylan further increased fecal propionate concentrations (p = 0.012, Friedman's test), an effect that showed two distinct groupings of temporal responses in participants.
32814582	3	51	with	adults	635:640	arg1	overweight					647:656	overweight	647:656	overweight	647:656	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	3	51	with	adults	635:640	arg1	obesity					670:676	class-I obesity	662:676	class-I obesity	662:676	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	2	52	theme	ecological	463:472	arg1	perspective					474:484	an ecological perspective	460:484	an ecological perspective	460:484	Our understanding of what drives this individuality is vastly incomplete and will require an ecological perspective as microbiomes function as complex inter-connected communities.
32814582	3	53	from	doses	775:779	arg1	acid					868:871	short-chain fatty acid	850:871	short-chain fatty acid	850:871	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	3	53	from	doses	775:779	arg1	n					743:743	n = 15	743:748	n = 15	743:748	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	3	53	from	doses	775:779	arg1	long-chain					709:718	long-chain	709:718	long-chain	709:718	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	3	53	from	doses	775:779	arg1	arabinoxylan					729:740	complex arabinoxylan	721:740	complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition	721:844	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	0	54	theme	Gut	0:2	arg1	modulation					15:24	Gut microbiota modulation	0:24	Gut microbiota modulation with long-chain corn bran arabinoxylan in adults with overweight and obesity	0:101	Gut microbiota modulation with long-chain corn bran arabinoxylan in adults with overweight and obesity is linked to an individualized temporal increase in fecal propionate.
32814582	3	55	theme	=	929:929	arg1	n					927:927	n = 16	927:932	n = 16	927:932	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	3	55	theme	=	929:929	arg1	cellulose					916:924	microcrystalline cellulose	899:924	microcrystalline cellulose (n = 16, non-fermentable control)	899:958	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	10	56	theme	ecological	2431:2440	arg1	framework					2442:2450	an ecological framework	2428:2450	an ecological framework that considers features of the wider gut microbial community for the prediction of metabolic outcomes of dietary fiber fermentation	2428:2582	These findings constitute a proof-of-concept for the merit of an ecological framework that considers features of the wider gut microbial community for the prediction of metabolic outcomes of dietary fiber fermentation.
32814582	11	57	theme	nutritional	2650:2660	arg1	application					2662:2672	nutritional application	2650:2672	nutritional application	2650:2672	This provides a basis to personalize the use of dietary fiber in nutritional application and to stratify human populations by relevant gut microbiota features to account for the inconsistent health effects in human intervention studies.
32814582	5	58	theme	propionate	1314:1323	arg1	concentrations					1325:1338	fecal propionate concentrations	1308:1338	fecal propionate concentrations (p = 0.012, Friedman's test)	1308:1367	Arabinoxylan further increased fecal propionate concentrations (p = 0.012, Friedman's test), an effect that showed two distinct groupings of temporal responses in participants.
32814582	5	58	theme	propionate	1314:1323	arg1	0.012					1345:1349	p = 0.012	1341:1349	p = 0.012	1341:1349	Arabinoxylan further increased fecal propionate concentrations (p = 0.012, Friedman's test), an effect that showed two distinct groupings of temporal responses in participants.
32814582	5	58	theme	propionate	1314:1323	arg1	effect					1373:1378	an effect	1370:1378	an effect that showed two distinct groupings of temporal responses in participants	1370:1451	Arabinoxylan further increased fecal propionate concentrations (p = 0.012, Friedman's test), an effect that showed two distinct groupings of temporal responses in participants.
32814582	8	59	theme	long-chain	1994:2003	arg1	arabinoxylan					2005:2016	long-chain arabinoxylan	1994:2016	long-chain arabinoxylan	1994:2016	CONCLUSION This study showed that long-chain arabinoxylan modulates both microbiota composition and the output of health-relevant SCFAs, providing information for a more targeted application of this fiber.
32814582	8	60	theme	health-relevant	2074:2088	arg1	SCFAs					2090:2094	health-relevant SCFAs	2074:2094	health-relevant SCFAs	2074:2094	CONCLUSION This study showed that long-chain arabinoxylan modulates both microbiota composition and the output of health-relevant SCFAs, providing information for a more targeted application of this fiber.
32814582	11	61	theme	fiber	2641:2645	arg1	use					2626:2628	the use	2622:2628	the use of dietary fiber in nutritional application	2622:2672	This provides a basis to personalize the use of dietary fiber in nutritional application and to stratify human populations by relevant gut microbiota features to account for the inconsistent health effects in human intervention studies.
32814582	0	62	theme	long-chain	31:40	arg1	arabinoxylan					52:63	long-chain corn bran arabinoxylan	31:63	long-chain corn bran arabinoxylan	31:63	Gut microbiota modulation with long-chain corn bran arabinoxylan in adults with overweight and obesity is linked to an individualized temporal increase in fecal propionate.
32814582	7	63	theme	single	1841:1846	arg1	taxa					1848:1851	single taxa	1841:1851	single taxa	1841:1851	Principal components (PCs) derived from community data were better predictors in MLR models as compared to single taxa, indicating that arabinoxylan fermentation is the result of multi-species interactions within microbiomes.
32814582	11	64	dep	basis	2601:2605	arg1	personalize					2610:2620	personalize	2610:2620	to personalize the use of dietary fiber in nutritional application	2607:2672	This provides a basis to personalize the use of dietary fiber in nutritional application and to stratify human populations by relevant gut microbiota features to account for the inconsistent health effects in human intervention studies.
32814582	11	64	dep	basis	2601:2605	arg1	stratify					2681:2688	stratify	2681:2688	to stratify human populations by relevant gut microbiota features to account for the inconsistent health effects in human intervention studies	2678:2819	This provides a basis to personalize the use of dietary fiber in nutritional application and to stratify human populations by relevant gut microbiota features to account for the inconsistent health effects in human intervention studies.
32814582	5	65	dep	0.012	1345:1349	arg1	test					1363:1366	Friedman's test	1352:1366	Friedman's test	1352:1366	Arabinoxylan further increased fecal propionate concentrations (p = 0.012, Friedman's test), an effect that showed two distinct groupings of temporal responses in participants.
32814582	11	66	theme	intervention	2800:2811	arg1	studies					2813:2819	human intervention studies	2794:2819	human intervention studies	2794:2819	This provides a basis to personalize the use of dietary fiber in nutritional application and to stratify human populations by relevant gut microbiota features to account for the inconsistent health effects in human intervention studies.
32814582	0	67	with	modulation	15:24	arg1	arabinoxylan					52:63	long-chain corn bran arabinoxylan	31:63	long-chain corn bran arabinoxylan	31:63	Gut microbiota modulation with long-chain corn bran arabinoxylan in adults with overweight and obesity is linked to an individualized temporal increase in fecal propionate.
32814582	9	68	theme	microbial	2310:2318	arg1	community					2320:2328	the global microbial community	2299:2328	the global microbial community showing the strongest associations	2299:2363	Variation in propionate production was linked to both compositional shifts and baseline composition, with PCs derived from shifts of the global microbial community showing the strongest associations.
32814582	0	69	theme	bran	47:50	arg1	arabinoxylan					52:63	long-chain corn bran arabinoxylan	31:63	long-chain corn bran arabinoxylan	31:63	Gut microbiota modulation with long-chain corn bran arabinoxylan in adults with overweight and obesity is linked to an individualized temporal increase in fecal propionate.
32814582	6	70	from	differences	1476:1486	arg1	shifts					1505:1510	compositional shifts	1491:1510	compositional shifts of the microbiota (p ≤ 0.025, PERMANOVA)	1491:1551	The two groups showed differences in compositional shifts of the microbiota (p ≤ 0.025, PERMANOVA), and multiple linear regression (MLR) analyses revealed that the propionate response was predictable through shifts and, to a lesser degree, baseline composition of the microbiota.
32814582	5	71	from	groupings	1405:1413	arg1	participants					1440:1451	participants	1440:1451	participants	1440:1451	Arabinoxylan further increased fecal propionate concentrations (p = 0.012, Friedman's test), an effect that showed two distinct groupings of temporal responses in participants.
32814582	9	72	attach	linked	2205:2210	arg1	shifts					2234:2239	compositional shifts	2220:2239	compositional shifts	2220:2239	Variation in propionate production was linked to both compositional shifts and baseline composition, with PCs derived from shifts of the global microbial community showing the strongest associations.
32814582	9	72	attach	linked	2205:2210	arg1	composition					2254:2264	baseline composition	2245:2264	baseline composition	2245:2264	Variation in propionate production was linked to both compositional shifts and baseline composition, with PCs derived from shifts of the global microbial community showing the strongest associations.
32814582	9	72	attach	linked	2205:2210	arg2	Variation					2166:2174	Variation	2166:2174	Variation in propionate production	2166:2199	Variation in propionate production was linked to both compositional shifts and baseline composition, with PCs derived from shifts of the global microbial community showing the strongest associations.
32814582	1	73	theme	dietary	221:227	arg1	fiber					229:233	dietary fiber	221:233	dietary fiber	221:233	BACKGROUND Variability in the health effects of dietary fiber might arise from inter-individual differences in the gut microbiota's ability to ferment these substrates into beneficial metabolites.
32814582	7	74	theme	community	1774:1782	arg1	data					1784:1787	community data	1774:1787	community data	1774:1787	Principal components (PCs) derived from community data were better predictors in MLR models as compared to single taxa, indicating that arabinoxylan fermentation is the result of multi-species interactions within microbiomes.
32814582	4	75	theme	bacterial	1077:1085	arg1	composition					1097:1107	fecal bacterial community composition	1071:1107	fecal bacterial community composition	1071:1107	RESULTS Arabinoxylan resulted in a global shift in fecal bacterial community composition, reduced α-diversity, and the promotion of specific taxa, including operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri.
32814582	9	76	theme	strongest	2342:2350	arg1	associations					2352:2363	the strongest associations	2338:2363	the strongest associations	2338:2363	Variation in propionate production was linked to both compositional shifts and baseline composition, with PCs derived from shifts of the global microbial community showing the strongest associations.
32814582	11	77	theme	inconsistent	2763:2774	arg1	effects					2783:2789	the inconsistent health effects	2759:2789	the inconsistent health effects in human intervention studies	2759:2819	This provides a basis to personalize the use of dietary fiber in nutritional application and to stratify human populations by relevant gut microbiota features to account for the inconsistent health effects in human intervention studies.
32814582	8	78	theme	targeted	2130:2137	arg1	application					2139:2149	a more targeted application	2123:2149	a more targeted application of this fiber	2123:2163	CONCLUSION This study showed that long-chain arabinoxylan modulates both microbiota composition and the output of health-relevant SCFAs, providing information for a more targeted application of this fiber.
32814582	3	79	from	long-chain	709:718	arg1	composition					834:844	gut microbiota composition	819:844	gut microbiota composition	819:844	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	12	80	dep	TRIAL	2822:2826	arg1	Clinicaltrials.gov					2841:2858	Clinicaltrials.gov	2841:2858	Clinicaltrials.gov	2841:2858	TRIAL REGISTRATION Clinicaltrials.gov, NCT02322112 , registered on July 3, 2015.
32814582	4	81	theme	global	1055:1060	arg1	shift					1062:1066	a global shift	1053:1066	a global shift in fecal bacterial community composition, reduced α-diversity, and the promotion of specific taxa, including operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri	1053:1274	RESULTS Arabinoxylan resulted in a global shift in fecal bacterial community composition, reduced α-diversity, and the promotion of specific taxa, including operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri.
32814582	3	82	theme	arabinoxylan	729:740	arg1	production					873:882	long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production	709:882	long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production	709:882	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	5	83	theme	temporal	1418:1425	arg1	responses					1427:1435	temporal responses	1418:1435	temporal responses in participants	1418:1451	Arabinoxylan further increased fecal propionate concentrations (p = 0.012, Friedman's test), an effect that showed two distinct groupings of temporal responses in participants.
32814582	3	84	theme	parallel	571:578	arg1	trial					623:627	exploratory randomized controlled trial	589:627	exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control)	589:958	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	3	84	theme	parallel	571:578	arg1	two-arm					580:586	a parallel two-arm	569:586	a parallel two-arm	569:586	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	9	85	attach	derived	2276:2282	arg1	shifts					2289:2294	shifts	2289:2294	shifts of the global microbial community showing the strongest associations	2289:2363	Variation in propionate production was linked to both compositional shifts and baseline composition, with PCs derived from shifts of the global microbial community showing the strongest associations.
32814582	9	85	attach	derived	2276:2282	arg2	PCs					2272:2274	PCs	2272:2274	PCs derived from shifts of the global microbial community showing the strongest associations	2272:2363	Variation in propionate production was linked to both compositional shifts and baseline composition, with PCs derived from shifts of the global microbial community showing the strongest associations.
32814582	3	86	from	acid	868:871	arg1	composition					834:844	gut microbiota composition	819:844	gut microbiota composition	819:844	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	0	87	from	increase	143:150	arg1	propionate					161:170	fecal propionate	155:170	fecal propionate	155:170	Gut microbiota modulation with long-chain corn bran arabinoxylan in adults with overweight and obesity is linked to an individualized temporal increase in fecal propionate.
32814582	9	88	from	Variation	2166:2174	arg1	production					2190:2199	propionate production	2179:2199	propionate production	2179:2199	Variation in propionate production was linked to both compositional shifts and baseline composition, with PCs derived from shifts of the global microbial community showing the strongest associations.
32814582	8	89	theme	microbiota	2033:2042	arg1	composition					2044:2054	microbiota composition	2033:2054	microbiota composition	2033:2054	CONCLUSION This study showed that long-chain arabinoxylan modulates both microbiota composition and the output of health-relevant SCFAs, providing information for a more targeted application of this fiber.
32814582	10	90	theme	microbial	2493:2501	arg1	community					2503:2511	the wider gut microbial community	2479:2511	the wider gut microbial community for the prediction of metabolic outcomes of dietary fiber fermentation	2479:2582	These findings constitute a proof-of-concept for the merit of an ecological framework that considers features of the wider gut microbial community for the prediction of metabolic outcomes of dietary fiber fermentation.
32814582	3	91	theme	randomized	601:610	arg1	trial					623:627	exploratory randomized controlled trial	589:627	exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control)	589:958	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	3	91	theme	randomized	601:610	arg1	two-arm					580:586	a parallel two-arm	569:586	a parallel two-arm	569:586	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	6	92	theme	microbiota	1519:1528	arg1	shifts					1505:1510	compositional shifts	1491:1510	compositional shifts of the microbiota (p ≤ 0.025, PERMANOVA)	1491:1551	The two groups showed differences in compositional shifts of the microbiota (p ≤ 0.025, PERMANOVA), and multiple linear regression (MLR) analyses revealed that the propionate response was predictable through shifts and, to a lesser degree, baseline composition of the microbiota.
32814582	3	93	dep	doses	775:779	arg1	female					782:787	female	782:787	female: 25 g/day; male: 35 g/day	782:813	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	10	94	theme	wider	2483:2487	arg1	community					2503:2511	the wider gut microbial community	2479:2511	the wider gut microbial community for the prediction of metabolic outcomes of dietary fiber fermentation	2479:2582	These findings constitute a proof-of-concept for the merit of an ecological framework that considers features of the wider gut microbial community for the prediction of metabolic outcomes of dietary fiber fermentation.
32814582	1	95	from	differences	269:279	arg1	ability					305:311	the gut microbiota's ability to ferment these substrates into beneficial metabolites	284:367	the gut microbiota's ability to ferment these substrates into beneficial metabolites	284:367	BACKGROUND Variability in the health effects of dietary fiber might arise from inter-individual differences in the gut microbiota's ability to ferment these substrates into beneficial metabolites.
32814582	5	96	from	participants	1440:1451	arg1	groupings					1405:1413	two distinct groupings	1392:1413	two distinct groupings of temporal responses in participants	1392:1451	Arabinoxylan further increased fecal propionate concentrations (p = 0.012, Friedman's test), an effect that showed two distinct groupings of temporal responses in participants.
32814582	11	97	theme	microbiota	2724:2733	arg1	features					2735:2742	relevant gut microbiota features	2711:2742	relevant gut microbiota features	2711:2742	This provides a basis to personalize the use of dietary fiber in nutritional application and to stratify human populations by relevant gut microbiota features to account for the inconsistent health effects in human intervention studies.
32814582	4	98	theme	reduced	1110:1116	arg1	α-diversity					1118:1128	reduced α-diversity	1110:1128	reduced α-diversity	1110:1128	RESULTS Arabinoxylan resulted in a global shift in fecal bacterial community composition, reduced α-diversity, and the promotion of specific taxa, including operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri.
32814582	11	99	theme	human	2794:2798	arg1	studies					2813:2819	human intervention studies	2794:2819	human intervention studies	2794:2819	This provides a basis to personalize the use of dietary fiber in nutritional application and to stratify human populations by relevant gut microbiota features to account for the inconsistent health effects in human intervention studies.
32814582	3	100	theme	supplementation	759:773	arg1	doses					775:779	high supplementation doses	754:779	high supplementation doses (female: 25 g/day; male: 35 g/day)	754:814	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	0	101	theme	temporal	134:141	arg1	increase					143:150	an individualized temporal increase	116:150	an individualized temporal increase in fecal propionate	116:170	Gut microbiota modulation with long-chain corn bran arabinoxylan in adults with overweight and obesity is linked to an individualized temporal increase in fecal propionate.
32814582	6	102	theme	compositional	1491:1503	arg1	shifts					1505:1510	compositional shifts	1491:1510	compositional shifts of the microbiota (p ≤ 0.025, PERMANOVA)	1491:1551	The two groups showed differences in compositional shifts of the microbiota (p ≤ 0.025, PERMANOVA), and multiple linear regression (MLR) analyses revealed that the propionate response was predictable through shifts and, to a lesser degree, baseline composition of the microbiota.
32814582	9	103	theme	baseline	2245:2252	arg1	composition					2254:2264	baseline composition	2245:2264	baseline composition	2245:2264	Variation in propionate production was linked to both compositional shifts and baseline composition, with PCs derived from shifts of the global microbial community showing the strongest associations.
32814582	6	104	theme	multiple	1558:1565	arg1	MLR					1586:1588	MLR	1586:1588	MLR	1586:1588	The two groups showed differences in compositional shifts of the microbiota (p ≤ 0.025, PERMANOVA), and multiple linear regression (MLR) analyses revealed that the propionate response was predictable through shifts and, to a lesser degree, baseline composition of the microbiota.
32814582	6	104	theme	multiple	1558:1565	arg1	regression					1574:1583	multiple linear regression	1558:1583	multiple linear regression (MLR) analyses	1558:1598	The two groups showed differences in compositional shifts of the microbiota (p ≤ 0.025, PERMANOVA), and multiple linear regression (MLR) analyses revealed that the propionate response was predictable through shifts and, to a lesser degree, baseline composition of the microbiota.
32814582	7	105	theme	interactions	1927:1938	arg1	fermentation					1883:1894	arabinoxylan fermentation	1870:1894	arabinoxylan fermentation	1870:1894	Principal components (PCs) derived from community data were better predictors in MLR models as compared to single taxa, indicating that arabinoxylan fermentation is the result of multi-species interactions within microbiomes.
32814582	7	105	theme	interactions	1927:1938	arg1	result					1903:1908	the result	1899:1908	the result of multi-species interactions within microbiomes	1899:1957	Principal components (PCs) derived from community data were better predictors in MLR models as compared to single taxa, indicating that arabinoxylan fermentation is the result of multi-species interactions within microbiomes.
32814582	7	106	theme	Principal	1734:1742	arg1	PCs					1756:1758	PCs	1756:1758	PCs	1756:1758	Principal components (PCs) derived from community data were better predictors in MLR models as compared to single taxa, indicating that arabinoxylan fermentation is the result of multi-species interactions within microbiomes.
32814582	7	106	theme	Principal	1734:1742	arg1	components					1744:1753	Principal components	1734:1753	Principal components (PCs) derived from community data	1734:1787	Principal components (PCs) derived from community data were better predictors in MLR models as compared to single taxa, indicating that arabinoxylan fermentation is the result of multi-species interactions within microbiomes.
32814582	4	107	theme	taxonomic	1189:1197	arg1	units					1199:1203	operational taxonomic units	1177:1203	operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri	1177:1274	RESULTS Arabinoxylan resulted in a global shift in fecal bacterial community composition, reduced α-diversity, and the promotion of specific taxa, including operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri.
32814582	11	108	theme	relevant	2711:2718	arg1	features					2735:2742	relevant gut microbiota features	2711:2742	relevant gut microbiota features	2711:2742	This provides a basis to personalize the use of dietary fiber in nutritional application and to stratify human populations by relevant gut microbiota features to account for the inconsistent health effects in human intervention studies.
32814582	13	109	dep	Abstract	2909:2916	arg1	Video					2903:2907	Video	2903:2907	Video	2903:2907	Video Abstract.
32814582	3	110	theme	short-chain	850:860	arg1	acid					868:871	short-chain fatty acid	850:871	short-chain fatty acid	850:871	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	7	111	theme	better	1794:1799	arg1	predictors					1801:1810	better predictors	1794:1810	better predictors in MLR models	1794:1824	Principal components (PCs) derived from community data were better predictors in MLR models as compared to single taxa, indicating that arabinoxylan fermentation is the result of multi-species interactions within microbiomes.
32814582	10	112	theme	metabolic	2535:2543	arg1	outcomes					2545:2552	metabolic outcomes	2535:2552	metabolic outcomes of dietary fiber fermentation	2535:2582	These findings constitute a proof-of-concept for the merit of an ecological framework that considers features of the wider gut microbial community for the prediction of metabolic outcomes of dietary fiber fermentation.
32814582	3	113	dep	n	927:927	arg1	control					951:957	non-fermentable control	935:957	non-fermentable control	935:957	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	4	114	theme	taxa	1161:1164	arg1	promotion					1139:1147	the promotion	1135:1147	the promotion of specific taxa, including operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri	1135:1274	RESULTS Arabinoxylan resulted in a global shift in fecal bacterial community composition, reduced α-diversity, and the promotion of specific taxa, including operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri.
32814582	4	114	theme	taxa	1161:1164	arg1	composition					1097:1107	fecal bacterial community composition	1071:1107	fecal bacterial community composition	1071:1107	RESULTS Arabinoxylan resulted in a global shift in fecal bacterial community composition, reduced α-diversity, and the promotion of specific taxa, including operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri.
32814582	4	114	theme	taxa	1161:1164	arg1	α-diversity					1118:1128	reduced α-diversity	1110:1128	reduced α-diversity	1110:1128	RESULTS Arabinoxylan resulted in a global shift in fecal bacterial community composition, reduced α-diversity, and the promotion of specific taxa, including operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri.
32814582	6	115	theme	baseline	1694:1701	arg1	composition					1703:1713	baseline composition	1694:1713	baseline composition of the microbiota	1694:1731	The two groups showed differences in compositional shifts of the microbiota (p ≤ 0.025, PERMANOVA), and multiple linear regression (MLR) analyses revealed that the propionate response was predictable through shifts and, to a lesser degree, baseline composition of the microbiota.
32814582	10	116	theme	fiber	2565:2569	arg1	fermentation					2571:2582	dietary fiber fermentation	2557:2582	dietary fiber fermentation	2557:2582	These findings constitute a proof-of-concept for the merit of an ecological framework that considers features of the wider gut microbial community for the prediction of metabolic outcomes of dietary fiber fermentation.
32814582	3	117	theme	gut	819:821	arg1	composition					834:844	gut microbiota composition	819:844	gut microbiota composition	819:844	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	3	118	theme	acid	868:871	arg1	production					873:882	long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production	709:882	long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production	709:882	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	5	119	theme	p	1341:1341	arg1	concentrations					1325:1338	fecal propionate concentrations	1308:1338	fecal propionate concentrations (p = 0.012, Friedman's test)	1308:1367	Arabinoxylan further increased fecal propionate concentrations (p = 0.012, Friedman's test), an effect that showed two distinct groupings of temporal responses in participants.
32814582	5	119	theme	p	1341:1341	arg1	0.012					1345:1349	p = 0.012	1341:1349	p = 0.012	1341:1349	Arabinoxylan further increased fecal propionate concentrations (p = 0.012, Friedman's test), an effect that showed two distinct groupings of temporal responses in participants.
32814582	7	120	theme	arabinoxylan	1870:1881	arg1	fermentation					1883:1894	arabinoxylan fermentation	1870:1894	arabinoxylan fermentation	1870:1894	Principal components (PCs) derived from community data were better predictors in MLR models as compared to single taxa, indicating that arabinoxylan fermentation is the result of multi-species interactions within microbiomes.
32814582	7	120	theme	arabinoxylan	1870:1881	arg1	result					1903:1908	the result	1899:1908	the result of multi-species interactions within microbiomes	1899:1957	Principal components (PCs) derived from community data were better predictors in MLR models as compared to single taxa, indicating that arabinoxylan fermentation is the result of multi-species interactions within microbiomes.
32814582	4	121	theme	related	1205:1211	arg1	units					1199:1203	operational taxonomic units	1177:1203	operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri	1177:1274	RESULTS Arabinoxylan resulted in a global shift in fecal bacterial community composition, reduced α-diversity, and the promotion of specific taxa, including operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri.
32814582	0	122	theme	microbiota	4:13	arg1	modulation					15:24	Gut microbiota modulation	0:24	Gut microbiota modulation with long-chain corn bran arabinoxylan in adults with overweight and obesity	0:101	Gut microbiota modulation with long-chain corn bran arabinoxylan in adults with overweight and obesity is linked to an individualized temporal increase in fecal propionate.
32814582	10	123	theme	framework	2442:2450	arg1	merit					2419:2423	the merit	2415:2423	the merit of an ecological framework that considers features of the wider gut microbial community for the prediction of metabolic outcomes of dietary fiber fermentation	2415:2582	These findings constitute a proof-of-concept for the merit of an ecological framework that considers features of the wider gut microbial community for the prediction of metabolic outcomes of dietary fiber fermentation.
32814582	5	124	theme	fecal	1308:1312	arg1	concentrations					1325:1338	fecal propionate concentrations	1308:1338	fecal propionate concentrations (p = 0.012, Friedman's test)	1308:1367	Arabinoxylan further increased fecal propionate concentrations (p = 0.012, Friedman's test), an effect that showed two distinct groupings of temporal responses in participants.
32814582	5	124	theme	fecal	1308:1312	arg1	0.012					1345:1349	p = 0.012	1341:1349	p = 0.012	1341:1349	Arabinoxylan further increased fecal propionate concentrations (p = 0.012, Friedman's test), an effect that showed two distinct groupings of temporal responses in participants.
32814582	5	124	theme	fecal	1308:1312	arg1	effect					1373:1378	an effect	1370:1378	an effect that showed two distinct groupings of temporal responses in participants	1370:1451	Arabinoxylan further increased fecal propionate concentrations (p = 0.012, Friedman's test), an effect that showed two distinct groupings of temporal responses in participants.
32814582	1	125	theme	gut	288:290	arg1	ability					305:311	the gut microbiota's ability to ferment these substrates into beneficial metabolites	284:367	the gut microbiota's ability to ferment these substrates into beneficial metabolites	284:367	BACKGROUND Variability in the health effects of dietary fiber might arise from inter-individual differences in the gut microbiota's ability to ferment these substrates into beneficial metabolites.
32814582	6	126	theme	regression	1574:1583	arg1	analyses					1591:1598	multiple linear regression (MLR) analyses	1558:1598	multiple linear regression (MLR) analyses	1558:1598	The two groups showed differences in compositional shifts of the microbiota (p ≤ 0.025, PERMANOVA), and multiple linear regression (MLR) analyses revealed that the propionate response was predictable through shifts and, to a lesser degree, baseline composition of the microbiota.
32814582	8	127	theme	SCFAs	2090:2094	arg1	output					2064:2069	the output	2060:2069	the output of health-relevant SCFAs	2060:2094	CONCLUSION This study showed that long-chain arabinoxylan modulates both microbiota composition and the output of health-relevant SCFAs, providing information for a more targeted application of this fiber.
32814582	8	127	theme	SCFAs	2090:2094	arg1	composition					2044:2054	microbiota composition	2033:2054	microbiota composition	2033:2054	CONCLUSION This study showed that long-chain arabinoxylan modulates both microbiota composition and the output of health-relevant SCFAs, providing information for a more targeted application of this fiber.
32814582	4	128	theme	RESULTS	1020:1026	arg1	Arabinoxylan					1028:1039	RESULTS Arabinoxylan	1020:1039	RESULTS Arabinoxylan	1020:1039	RESULTS Arabinoxylan resulted in a global shift in fecal bacterial community composition, reduced α-diversity, and the promotion of specific taxa, including operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri.
32814582	11	129	theme	dietary	2633:2639	arg1	fiber					2641:2645	dietary fiber	2633:2645	dietary fiber	2633:2645	This provides a basis to personalize the use of dietary fiber in nutritional application and to stratify human populations by relevant gut microbiota features to account for the inconsistent health effects in human intervention studies.
32814582	0	130	theme	corn	42:45	arg1	arabinoxylan					52:63	long-chain corn bran arabinoxylan	31:63	long-chain corn bran arabinoxylan	31:63	Gut microbiota modulation with long-chain corn bran arabinoxylan in adults with overweight and obesity is linked to an individualized temporal increase in fecal propionate.
32814582	11	131	from	use	2626:2628	arg1	application					2662:2672	nutritional application	2650:2672	nutritional application	2650:2672	This provides a basis to personalize the use of dietary fiber in nutritional application and to stratify human populations by relevant gut microbiota features to account for the inconsistent health effects in human intervention studies.
32814582	7	132	from	predictors	1801:1810	arg1	models					1819:1824	MLR models	1815:1824	MLR models	1815:1824	Principal components (PCs) derived from community data were better predictors in MLR models as compared to single taxa, indicating that arabinoxylan fermentation is the result of multi-species interactions within microbiomes.
32814582	9	133	theme	global	2303:2308	arg1	community					2320:2328	the global microbial community	2299:2328	the global microbial community showing the strongest associations	2299:2363	Variation in propionate production was linked to both compositional shifts and baseline composition, with PCs derived from shifts of the global microbial community showing the strongest associations.
32814582	11	134	from	effects	2783:2789	arg1	studies					2813:2819	human intervention studies	2794:2819	human intervention studies	2794:2819	This provides a basis to personalize the use of dietary fiber in nutritional application and to stratify human populations by relevant gut microbiota features to account for the inconsistent health effects in human intervention studies.
32814582	6	135	theme	microbiota	1722:1731	arg1	composition					1703:1713	baseline composition	1694:1713	baseline composition of the microbiota	1694:1731	The two groups showed differences in compositional shifts of the microbiota (p ≤ 0.025, PERMANOVA), and multiple linear regression (MLR) analyses revealed that the propionate response was predictable through shifts and, to a lesser degree, baseline composition of the microbiota.
32814582	3	136	theme	long-chain	709:718	arg1	production					873:882	long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production	709:882	long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production	709:882	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	9	137	theme	community	2320:2328	arg1	shifts					2289:2294	shifts	2289:2294	shifts of the global microbial community showing the strongest associations	2289:2363	Variation in propionate production was linked to both compositional shifts and baseline composition, with PCs derived from shifts of the global microbial community showing the strongest associations.
32814582	7	138	theme	MLR	1815:1817	arg1	models					1819:1824	MLR models	1815:1824	MLR models	1815:1824	Principal components (PCs) derived from community data were better predictors in MLR models as compared to single taxa, indicating that arabinoxylan fermentation is the result of multi-species interactions within microbiomes.
32814582	3	139	theme	non-fermentable	935:949	arg1	control					951:957	non-fermentable control	935:957	non-fermentable control	935:957	Here, we performed a parallel two-arm, exploratory randomized controlled trial in 31 adults with overweight and class-I obesity to characterize the effects of long-chain, complex arabinoxylan (n = 15) at high supplementation doses (female: 25 g/day; male: 35 g/day) on gut microbiota composition and short-chain fatty acid production as compared to microcrystalline cellulose (n = 16, non-fermentable control), and integrated the findings using an ecological framework.
32814582	1	140	theme	fiber	229:233	arg1	effects					210:216	the health effects	199:216	the health effects of dietary fiber	199:233	BACKGROUND Variability in the health effects of dietary fiber might arise from inter-individual differences in the gut microbiota's ability to ferment these substrates into beneficial metabolites.
32814582	5	141	from	responses	1427:1435	arg1	participants					1440:1451	participants	1440:1451	participants	1440:1451	Arabinoxylan further increased fecal propionate concentrations (p = 0.012, Friedman's test), an effect that showed two distinct groupings of temporal responses in participants.
32814582	4	142	theme	fecal	1071:1075	arg1	composition					1097:1107	fecal bacterial community composition	1071:1107	fecal bacterial community composition	1071:1107	RESULTS Arabinoxylan resulted in a global shift in fecal bacterial community composition, reduced α-diversity, and the promotion of specific taxa, including operational taxonomic units related to Bifidobacterium longum, Blautia obeum, and Prevotella copri.
34011985	3	0	theme	antagonistic	518:529	arg1	capacity					531:538	its antagonistic capacity	514:538	its antagonistic capacity	514:538	Our results confirmed its antagonistic capacity on other yeast species isolated from fruits or flowers and demonstrated that the antagonistic capacity was well correlated with the size of the red pigmented zone.
34011985	1	1	theme	chemicals	267:275	arg1	approach					236:243	an alternative approach	221:243	an alternative approach	221:243	Biological control against microbial infections has a great potential as an alternative approach instead of fungicidal chemicals, which can cause environmental pollution.
34011985	10	2	theme	culture	1499:1505	arg1	media					1507:1511	the culture media	1495:1511	the culture media	1495:1511	In addition, our results suggest that other mechanisms besides the iron depletion of the culture media may contribute to the antagonistic capacity of M. andauensis.
34011985	9	3	theme	pH	1317:1318	arg1	effect					1307:1312	The effect	1303:1312	The effect of pH on pigment production	1303:1340	The effect of pH on pigment production varied depending of whether it was tested in liquid or solid media.
34011985	10	4	theme	other	1448:1452	arg1	mechanisms					1454:1463	other mechanisms	1448:1463	other mechanisms besides the iron depletion of the culture media	1448:1511	In addition, our results suggest that other mechanisms besides the iron depletion of the culture media may contribute to the antagonistic capacity of M. andauensis.
34011985	2	5	theme	antagonistic	380:391	arg1	yeasts					393:398	the antagonistic yeasts	376:398	the antagonistic yeasts	376:398	The pigment producer Metschnikowia andauensis belongs to the antagonistic yeasts, but details of the mechanism by which it inhibits growth of other microbes are less known.
34011985	6	6	theme	pulcherrimin	1006:1017	arg1	Production					971:980	Production	971:980	Production	971:980	Production and localisation of the pulcherrimin strongly depended on composition of the media and other culture factors.
34011985	6	6	theme	pulcherrimin	1006:1017	arg1	localisation					986:997	localisation	986:997	localisation	986:997	Production and localisation of the pulcherrimin strongly depended on composition of the media and other culture factors.
34011985	5	7	theme	properties	959:968	arg1	vineyards					920:928	vineyards	920:928	vineyards	920:928	Its production was possible even in the presence of 0.05 mg/ml copper sulphate, which is widely used in organic vineyards because of its antimicrobial properties.
34011985	9	8	theme	pigment	1323:1329	arg1	production					1331:1340	pigment production	1323:1340	pigment production	1323:1340	The effect of pH on pigment production varied depending of whether it was tested in liquid or solid media.
34011985	3	9	theme	yeast	549:553	arg1	species					555:561	other yeast species	543:561	other yeast species isolated from fruits or flowers	543:593	Our results confirmed its antagonistic capacity on other yeast species isolated from fruits or flowers and demonstrated that the antagonistic capacity was well correlated with the size of the red pigmented zone.
34011985	6	10	theme	factors	1083:1089	arg1	composition					1040:1050	composition	1040:1050	composition of the media and other culture factors	1040:1089	Production and localisation of the pulcherrimin strongly depended on composition of the media and other culture factors.
34011985	2	11	theme	mechanism	420:428	arg1	details					405:411	details	405:411	details of the mechanism by which it inhibits growth of other microbes	405:474	The pigment producer Metschnikowia andauensis belongs to the antagonistic yeasts, but details of the mechanism by which it inhibits growth of other microbes are less known.
34011985	10	12	theme	andauensis	1563:1572	arg1	capacity					1548:1555	the antagonistic capacity	1531:1555	the antagonistic capacity of M. andauensis	1531:1572	In addition, our results suggest that other mechanisms besides the iron depletion of the culture media may contribute to the antagonistic capacity of M. andauensis.
34011985	9	13	dep	tested	1377:1382	arg1	depending					1349:1357	depending	1349:1357	depending	1349:1357	The effect of pH on pigment production varied depending of whether it was tested in liquid or solid media.
34011985	7	14	theme	certain	1156:1162	arg1	acids					1170:1174	certain amino acids	1156:1174	certain amino acids	1156:1174	Glucose, galactose, disaccharides and the presence of pectin or certain amino acids clearly promoted pigment production.
34011985	7	15	theme	pigment	1193:1199	arg1	production					1201:1210	pigment production	1193:1210	pigment production	1193:1210	Glucose, galactose, disaccharides and the presence of pectin or certain amino acids clearly promoted pigment production.
34011985	5	16	dep	sulphate	878:885	arg1	presence					848:855	presence	848:855	presence	848:855	Its production was possible even in the presence of 0.05 mg/ml copper sulphate, which is widely used in organic vineyards because of its antimicrobial properties.
34011985	5	16	dep	sulphate	878:885	arg1	the					844:846	the	844:846	the	844:846	Its production was possible even in the presence of 0.05 mg/ml copper sulphate, which is widely used in organic vineyards because of its antimicrobial properties.
34011985	5	17	theme	copper	871:876	arg1	sulphate					878:885	0.05 mg/ml copper sulphate	860:885	0.05 mg/ml copper sulphate	860:885	Its production was possible even in the presence of 0.05 mg/ml copper sulphate, which is widely used in organic vineyards because of its antimicrobial properties.
34011985	0	18	theme	antagonistic	4:15	arg1	andauensis					31:40	The antagonistic Metschnikowia andauensis	0:40	The antagonistic Metschnikowia andauensis	0:40	The antagonistic Metschnikowia andauensis produces extracellular enzymes and pulcherrimin, whose production can be promoted by the culture factors.
34011985	10	19	theme	antagonistic	1535:1546	arg1	capacity					1548:1555	the antagonistic capacity	1531:1555	the antagonistic capacity of M. andauensis	1531:1572	In addition, our results suggest that other mechanisms besides the iron depletion of the culture media may contribute to the antagonistic capacity of M. andauensis.
34011985	2	20	theme	Metschnikowia	340:352	arg1	andauensis					354:363	The pigment producer Metschnikowia andauensis	319:363	The pigment producer Metschnikowia andauensis	319:363	The pigment producer Metschnikowia andauensis belongs to the antagonistic yeasts, but details of the mechanism by which it inhibits growth of other microbes are less known.
34011985	5	21	theme	sulphate	878:885	arg1	possible					827:834	possible	827:834	possible	827:834	Its production was possible even in the presence of 0.05 mg/ml copper sulphate, which is widely used in organic vineyards because of its antimicrobial properties.
34011985	2	22	theme	other	461:465	arg1	microbes					467:474	other microbes	461:474	other microbes	461:474	The pigment producer Metschnikowia andauensis belongs to the antagonistic yeasts, but details of the mechanism by which it inhibits growth of other microbes are less known.
34011985	2	23	theme	producer	331:338	arg1	andauensis					354:363	The pigment producer Metschnikowia andauensis	319:363	The pigment producer Metschnikowia andauensis	319:363	The pigment producer Metschnikowia andauensis belongs to the antagonistic yeasts, but details of the mechanism by which it inhibits growth of other microbes are less known.
34011985	1	24	theme	great	202:206	arg1	potential					208:216	a great potential	200:216	a great potential as an alternative approach instead of fungicidal chemicals, which can cause environmental pollution	200:316	Biological control against microbial infections has a great potential as an alternative approach instead of fungicidal chemicals, which can cause environmental pollution.
34011985	7	25	theme	acids	1170:1174	arg1	disaccharides					1112:1124	disaccharides	1112:1124	disaccharides	1112:1124	Glucose, galactose, disaccharides and the presence of pectin or certain amino acids clearly promoted pigment production.
34011985	7	25	theme	acids	1170:1174	arg1	presence					1134:1141	the presence	1130:1141	the presence of pectin or certain amino acids	1130:1174	Glucose, galactose, disaccharides and the presence of pectin or certain amino acids clearly promoted pigment production.
34011985	7	25	theme	acids	1170:1174	arg1	galactose					1101:1109	galactose	1101:1109	galactose	1101:1109	Glucose, galactose, disaccharides and the presence of pectin or certain amino acids clearly promoted pigment production.
34011985	7	25	theme	acids	1170:1174	arg1	Glucose					1092:1098	Glucose	1092:1098	Glucose	1092:1098	Glucose, galactose, disaccharides and the presence of pectin or certain amino acids clearly promoted pigment production.
34011985	1	26	theme	environmental	294:306	arg1	pollution					308:316	environmental pollution	294:316	environmental pollution	294:316	Biological control against microbial infections has a great potential as an alternative approach instead of fungicidal chemicals, which can cause environmental pollution.
34011985	0	27	theme	culture	131:137	arg1	factors					139:145	the culture factors	127:145	the culture factors	127:145	The antagonistic Metschnikowia andauensis produces extracellular enzymes and pulcherrimin, whose production can be promoted by the culture factors.
34011985	2	28	theme	pigment	323:329	arg1	andauensis					354:363	The pigment producer Metschnikowia andauensis	319:363	The pigment producer Metschnikowia andauensis	319:363	The pigment producer Metschnikowia andauensis belongs to the antagonistic yeasts, but details of the mechanism by which it inhibits growth of other microbes are less known.
34011985	3	29	attach	isolated	563:570	arg1	flowers					587:593	flowers	587:593	flowers	587:593	Our results confirmed its antagonistic capacity on other yeast species isolated from fruits or flowers and demonstrated that the antagonistic capacity was well correlated with the size of the red pigmented zone.
34011985	3	29	attach	isolated	563:570	arg1	fruits					577:582	fruits	577:582	fruits	577:582	Our results confirmed its antagonistic capacity on other yeast species isolated from fruits or flowers and demonstrated that the antagonistic capacity was well correlated with the size of the red pigmented zone.
34011985	3	29	attach	isolated	563:570	arg2	species					555:561	other yeast species	543:561	other yeast species isolated from fruits or flowers	543:593	Our results confirmed its antagonistic capacity on other yeast species isolated from fruits or flowers and demonstrated that the antagonistic capacity was well correlated with the size of the red pigmented zone.
34011985	6	30	theme	other	1069:1073	arg1	factors					1083:1089	other culture factors	1069:1089	other culture factors	1069:1089	Production and localisation of the pulcherrimin strongly depended on composition of the media and other culture factors.
34011985	0	31	theme	Metschnikowia	17:29	arg1	andauensis					31:40	The antagonistic Metschnikowia andauensis	0:40	The antagonistic Metschnikowia andauensis	0:40	The antagonistic Metschnikowia andauensis produces extracellular enzymes and pulcherrimin, whose production can be promoted by the culture factors.
34011985	4	32	theme	iron	779:782	arg1	pigment					747:753	its red pigment	739:753	its red pigment	739:753	We have isolated and characterized its red pigment, which proved to be the iron chelating pulcherrimin.
34011985	4	32	theme	iron	779:782	arg1	pulcherrimin					794:805	the iron chelating pulcherrimin	775:805	the iron chelating pulcherrimin	775:805	We have isolated and characterized its red pigment, which proved to be the iron chelating pulcherrimin.
34011985	1	33	theme	microbial	175:183	arg1	infections					185:194	microbial infections	175:194	microbial infections	175:194	Biological control against microbial infections has a great potential as an alternative approach instead of fungicidal chemicals, which can cause environmental pollution.
34011985	8	34	theme	pigmented	1286:1294	arg1	zones					1296:1300	red pigmented zones	1282:1300	red pigmented zones	1282:1300	Higher temperatures and iron concentration decreased the diameter of red pigmented zones.
34011985	0	35	theme	extracellular	51:63	arg1	enzymes					65:71	extracellular enzymes	51:71	extracellular enzymes	51:71	The antagonistic Metschnikowia andauensis produces extracellular enzymes and pulcherrimin, whose production can be promoted by the culture factors.
34011985	5	36	used	used	904:907	arg2	sulphate					878:885	0.05 mg/ml copper sulphate	860:885	0.05 mg/ml copper sulphate	860:885	Its production was possible even in the presence of 0.05 mg/ml copper sulphate, which is widely used in organic vineyards because of its antimicrobial properties.
34011985	6	37	theme	culture	1075:1081	arg1	factors					1083:1089	other culture factors	1069:1089	other culture factors	1069:1089	Production and localisation of the pulcherrimin strongly depended on composition of the media and other culture factors.
34011985	6	38	theme	media	1059:1063	arg1	composition					1040:1050	composition	1040:1050	composition of the media and other culture factors	1040:1089	Production and localisation of the pulcherrimin strongly depended on composition of the media and other culture factors.
34011985	5	39	theme	0.05 mg/ml	860:869	arg1	sulphate					878:885	0.05 mg/ml copper sulphate	860:885	0.05 mg/ml copper sulphate	860:885	Its production was possible even in the presence of 0.05 mg/ml copper sulphate, which is widely used in organic vineyards because of its antimicrobial properties.
34011985	7	40	theme	amino	1164:1168	arg1	acids					1170:1174	certain amino acids	1156:1174	certain amino acids	1156:1174	Glucose, galactose, disaccharides and the presence of pectin or certain amino acids clearly promoted pigment production.
34011985	1	41	contain	has	196:198	arg1	control					159:165	Biological control	148:165	Biological control against microbial infections	148:194	Biological control against microbial infections has a great potential as an alternative approach instead of fungicidal chemicals, which can cause environmental pollution.
34011985	1	41	contain	has	196:198	arg2	potential					208:216	a great potential	200:216	a great potential as an alternative approach instead of fungicidal chemicals, which can cause environmental pollution	200:316	Biological control against microbial infections has a great potential as an alternative approach instead of fungicidal chemicals, which can cause environmental pollution.
34011985	3	42	theme	antagonistic	621:632	arg1	capacity					634:641	the antagonistic capacity	617:641	the antagonistic capacity	617:641	Our results confirmed its antagonistic capacity on other yeast species isolated from fruits or flowers and demonstrated that the antagonistic capacity was well correlated with the size of the red pigmented zone.
34011985	1	43	theme	alternative	224:234	arg1	approach					236:243	an alternative approach	221:243	an alternative approach	221:243	Biological control against microbial infections has a great potential as an alternative approach instead of fungicidal chemicals, which can cause environmental pollution.
34011985	7	44	theme	pectin	1146:1151	arg1	disaccharides					1112:1124	disaccharides	1112:1124	disaccharides	1112:1124	Glucose, galactose, disaccharides and the presence of pectin or certain amino acids clearly promoted pigment production.
34011985	7	44	theme	pectin	1146:1151	arg1	presence					1134:1141	the presence	1130:1141	the presence of pectin or certain amino acids	1130:1174	Glucose, galactose, disaccharides and the presence of pectin or certain amino acids clearly promoted pigment production.
34011985	7	44	theme	pectin	1146:1151	arg1	galactose					1101:1109	galactose	1101:1109	galactose	1101:1109	Glucose, galactose, disaccharides and the presence of pectin or certain amino acids clearly promoted pigment production.
34011985	7	44	theme	pectin	1146:1151	arg1	Glucose					1092:1098	Glucose	1092:1098	Glucose	1092:1098	Glucose, galactose, disaccharides and the presence of pectin or certain amino acids clearly promoted pigment production.
34011985	9	45	theme	liquid	1387:1392	arg1	media					1403:1407	liquid or solid media	1387:1407	liquid or solid media	1387:1407	The effect of pH on pigment production varied depending of whether it was tested in liquid or solid media.
34011985	10	46	theme	media	1507:1511	arg1	depletion					1482:1490	the iron depletion	1473:1490	the iron depletion of the culture media	1473:1511	In addition, our results suggest that other mechanisms besides the iron depletion of the culture media may contribute to the antagonistic capacity of M. andauensis.
34011985	3	47	theme	pigmented	688:696	arg1	zone					698:701	the red pigmented zone	680:701	the red pigmented zone	680:701	Our results confirmed its antagonistic capacity on other yeast species isolated from fruits or flowers and demonstrated that the antagonistic capacity was well correlated with the size of the red pigmented zone.
34011985	10	48	theme	iron	1477:1480	arg1	depletion					1482:1490	the iron depletion	1473:1490	the iron depletion of the culture media	1473:1511	In addition, our results suggest that other mechanisms besides the iron depletion of the culture media may contribute to the antagonistic capacity of M. andauensis.
34011985	8	49	theme	iron	1237:1240	arg1	concentration					1242:1254	iron concentration	1237:1254	iron concentration	1237:1254	Higher temperatures and iron concentration decreased the diameter of red pigmented zones.
34011985	3	50	theme	zone	698:701	arg1	size					672:675	the size	668:675	the size of the red pigmented zone	668:701	Our results confirmed its antagonistic capacity on other yeast species isolated from fruits or flowers and demonstrated that the antagonistic capacity was well correlated with the size of the red pigmented zone.
34011985	8	51	theme	red	1282:1284	arg1	zones					1296:1300	red pigmented zones	1282:1300	red pigmented zones	1282:1300	Higher temperatures and iron concentration decreased the diameter of red pigmented zones.
34011985	4	52	theme	red	743:745	arg1	pigment					747:753	its red pigment	739:753	its red pigment	739:753	We have isolated and characterized its red pigment, which proved to be the iron chelating pulcherrimin.
34011985	4	52	theme	red	743:745	arg1	pulcherrimin					794:805	the iron chelating pulcherrimin	775:805	the iron chelating pulcherrimin	775:805	We have isolated and characterized its red pigment, which proved to be the iron chelating pulcherrimin.
34011985	5	53	theme	antimicrobial	945:957	arg1	properties					959:968	its antimicrobial properties	941:968	its antimicrobial properties	941:968	Its production was possible even in the presence of 0.05 mg/ml copper sulphate, which is widely used in organic vineyards because of its antimicrobial properties.
34011985	9	54	theme	solid	1397:1401	arg1	media					1403:1407	liquid or solid media	1387:1407	liquid or solid media	1387:1407	The effect of pH on pigment production varied depending of whether it was tested in liquid or solid media.
34011985	4	55	theme	chelating	784:792	arg1	pigment					747:753	its red pigment	739:753	its red pigment	739:753	We have isolated and characterized its red pigment, which proved to be the iron chelating pulcherrimin.
34011985	4	55	theme	chelating	784:792	arg1	pulcherrimin					794:805	the iron chelating pulcherrimin	775:805	the iron chelating pulcherrimin	775:805	We have isolated and characterized its red pigment, which proved to be the iron chelating pulcherrimin.
34011985	3	56	theme	other	543:547	arg1	species					555:561	other yeast species	543:561	other yeast species isolated from fruits or flowers	543:593	Our results confirmed its antagonistic capacity on other yeast species isolated from fruits or flowers and demonstrated that the antagonistic capacity was well correlated with the size of the red pigmented zone.
34011985	10	57	theme	M.	1560:1561	arg1	andauensis					1563:1572	M. andauensis	1560:1572	M. andauensis	1560:1572	In addition, our results suggest that other mechanisms besides the iron depletion of the culture media may contribute to the antagonistic capacity of M. andauensis.
34011985	3	58	theme	red	684:686	arg1	zone					698:701	the red pigmented zone	680:701	the red pigmented zone	680:701	Our results confirmed its antagonistic capacity on other yeast species isolated from fruits or flowers and demonstrated that the antagonistic capacity was well correlated with the size of the red pigmented zone.
34011985	8	59	theme	zones	1296:1300	arg1	diameter					1270:1277	the diameter	1266:1277	the diameter of red pigmented zones	1266:1300	Higher temperatures and iron concentration decreased the diameter of red pigmented zones.
34011985	1	60	theme	Biological	148:157	arg1	control					159:165	Biological control	148:165	Biological control against microbial infections	148:194	Biological control against microbial infections has a great potential as an alternative approach instead of fungicidal chemicals, which can cause environmental pollution.
34011985	9	61	from	effect	1307:1312	arg1	production					1331:1340	pigment production	1323:1340	pigment production	1323:1340	The effect of pH on pigment production varied depending of whether it was tested in liquid or solid media.
34011985	8	62	theme	Higher	1213:1218	arg1	temperatures					1220:1231	Higher temperatures	1213:1231	Higher temperatures	1213:1231	Higher temperatures and iron concentration decreased the diameter of red pigmented zones.
34011985	2	63	theme	microbes	467:474	arg1	growth					451:456	growth	451:456	growth of other microbes	451:474	The pigment producer Metschnikowia andauensis belongs to the antagonistic yeasts, but details of the mechanism by which it inhibits growth of other microbes are less known.
34011985	1	64	theme	fungicidal	256:265	arg1	chemicals					267:275	fungicidal chemicals	256:275	fungicidal chemicals	256:275	Biological control against microbial infections has a great potential as an alternative approach instead of fungicidal chemicals, which can cause environmental pollution.
34639165	6	0	theme	flame	1170:1174	arg1	characteristics					1187:1201	improved flame resistance characteristics	1161:1201	improved flame resistance characteristics	1161:1201	Most importantly, the polyurethane composites filled with vermiculite filler and modified vermiculite fillers exhibited improved flame resistance characteristics (the value of total smoke release was reduced by ~34%, the value of peak heat release was reduced by ~25%).
34639165	3	1	dep	flame-retardant	487:501	arg1	release					558:564	heat peak release	548:564	heat peak release	548:564	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	1	dep	flame-retardant	487:501	arg1	index					526:530	limiting oxygen index	510:530	limiting oxygen index	510:530	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	1	dep	flame-retardant	487:501	arg1	time					542:545	ignition time	533:545	ignition time	533:545	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	5	2	theme	compressive	931:941	arg1	strength					943:950	the foams' compressive strength	920:950	the foams' compressive strength	920:950	For example, after the addition of modified vermiculite fillers, the foams' compressive strength was enhanced by ~6-18%, their flexural strength by ~2-10%, and their toughness by ~1-5%.
34639165	6	3	theme	total	1217:1221	arg1	release					1229:1235	total smoke release	1217:1235	total smoke release	1217:1235	Most importantly, the polyurethane composites filled with vermiculite filler and modified vermiculite fillers exhibited improved flame resistance characteristics (the value of total smoke release was reduced by ~34%, the value of peak heat release was reduced by ~25%).
34639165	6	4	theme	improved	1161:1168	arg1	characteristics					1187:1201	improved flame resistance characteristics	1161:1201	improved flame resistance characteristics	1161:1201	Most importantly, the polyurethane composites filled with vermiculite filler and modified vermiculite fillers exhibited improved flame resistance characteristics (the value of total smoke release was reduced by ~34%, the value of peak heat release was reduced by ~25%).
34639165	6	5	theme	release	1281:1287	arg1	value					1262:1266	the value	1258:1266	the value of peak heat release	1258:1287	Most importantly, the polyurethane composites filled with vermiculite filler and modified vermiculite fillers exhibited improved flame resistance characteristics (the value of total smoke release was reduced by ~34%, the value of peak heat release was reduced by ~25%).
34639165	0	6	theme	Flame	75:79	arg1	Retardant					81:89	a Flame Retardant	73:89	a Flame Retardant for Polyurethane Foams	73:112	Vermiculite Filler Modified with Casein, Chitosan, and Potato Protein as a Flame Retardant for Polyurethane Foams.
34639165	5	7	theme	fillers	911:917	arg1	addition					878:885	the addition	874:885	the addition of modified vermiculite fillers	874:917	For example, after the addition of modified vermiculite fillers, the foams' compressive strength was enhanced by ~6-18%, their flexural strength by ~2-10%, and their toughness by ~1-5%.
34639165	3	8	theme	selected	317:324	arg1	thermal					432:438	thermal	432:438	thermal	432:438	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	8	theme	selected	317:324	arg1	properties					583:592	mechanical properties	572:592	mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength)	572:674	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	8	theme	selected	317:324	arg1	flame-retardant					487:501	flame-retardant	487:501	flame-retardant	487:501	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	8	theme	selected	317:324	arg1	rheological					381:391	rheological	381:391	rheological	381:391	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	8	theme	selected	317:324	arg1	properties					326:335	selected properties	317:335	selected properties	317:335	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	9	theme	decomposition	464:476	arg1	stages					478:483	thermal decomposition stages	456:483	thermal decomposition stages	456:483	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	6	10	theme	vermiculite	1099:1109	arg1	filler					1111:1116	vermiculite filler	1099:1116	vermiculite filler	1099:1116	Most importantly, the polyurethane composites filled with vermiculite filler and modified vermiculite fillers exhibited improved flame resistance characteristics (the value of total smoke release was reduced by ~34%, the value of peak heat release was reduced by ~25%).
34639165	3	11	theme	stages	478:483	arg1	temperature					441:451	temperature	441:451	temperature of thermal decomposition stages	441:483	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	6	12	theme	vermiculite	1131:1141	arg1	fillers					1143:1149	modified vermiculite fillers	1122:1149	modified vermiculite fillers	1122:1149	Most importantly, the polyurethane composites filled with vermiculite filler and modified vermiculite fillers exhibited improved flame resistance characteristics (the value of total smoke release was reduced by ~34%, the value of peak heat release was reduced by ~25%).
34639165	6	13	theme	release	1229:1235	arg1	value					1208:1212	the value	1204:1212	the value of total smoke release	1204:1235	Most importantly, the polyurethane composites filled with vermiculite filler and modified vermiculite fillers exhibited improved flame resistance characteristics (the value of total smoke release was reduced by ~34%, the value of peak heat release was reduced by ~25%).
34639165	3	14	theme	compressive	606:616	arg1	toughness					595:603	toughness	595:603	toughness	595:603	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	14	theme	compressive	606:616	arg1	strength					618:625	compressive strength	606:625	compressive strength (parallel and perpendicular)	606:654	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	6	15	theme	modified	1122:1129	arg1	fillers					1143:1149	modified vermiculite fillers	1122:1149	modified vermiculite fillers	1122:1149	Most importantly, the polyurethane composites filled with vermiculite filler and modified vermiculite fillers exhibited improved flame resistance characteristics (the value of total smoke release was reduced by ~34%, the value of peak heat release was reduced by ~25%).
34639165	3	16	theme	thermal	456:462	arg1	stages					478:483	thermal decomposition stages	456:483	thermal decomposition stages	456:483	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	17	theme	composites	353:362	arg1	thermal					432:438	thermal	432:438	thermal	432:438	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	17	theme	composites	353:362	arg1	properties					583:592	mechanical properties	572:592	mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength)	572:674	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	17	theme	composites	353:362	arg1	flame-retardant					487:501	flame-retardant	487:501	flame-retardant	487:501	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	17	theme	composites	353:362	arg1	rheological					381:391	rheological	381:391	rheological	381:391	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	17	theme	composites	353:362	arg1	properties					326:335	selected properties	317:335	selected properties	317:335	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	0	18	theme	Vermiculite	0:10	arg1	Filler					12:17	Vermiculite Filler	0:17	Vermiculite Filler	0:17	Vermiculite Filler Modified with Casein, Chitosan, and Potato Protein as a Flame Retardant for Polyurethane Foams.
34639165	3	19	dep	properties	583:592	arg1	toughness					595:603	toughness	595:603	toughness	595:603	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	19	dep	properties	583:592	arg1	strength					666:673	flexural strength	657:673	flexural strength	657:673	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	19	dep	properties	583:592	arg1	strength					618:625	compressive strength	606:625	compressive strength (parallel and perpendicular)	606:654	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	4	20	theme	greatest	747:754	arg1	improvement					756:766	the greatest improvement	743:766	the greatest improvement	743:766	Among all the modified polyurethane composites, the greatest improvement was noticed in the PU foams filled with vermiculite modified with casein and chitosan.
34639165	3	21	dep	thermal	432:438	arg1	temperature					441:451	temperature	441:451	temperature of thermal decomposition stages	441:483	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	1	22	theme	composite	148:156	arg1	foams					158:162	polyurethane (PU) composite foams	130:162	polyurethane (PU) composite foams	130:162	In this study, polyurethane (PU) composite foams were modified with 2 wt.
34639165	5	23	theme	modified	890:897	arg1	fillers					911:917	modified vermiculite fillers	890:917	modified vermiculite fillers	890:917	For example, after the addition of modified vermiculite fillers, the foams' compressive strength was enhanced by ~6-18%, their flexural strength by ~2-10%, and their toughness by ~1-5%.
34639165	6	24	theme	smoke	1223:1227	arg1	release					1229:1235	total smoke release	1217:1235	total smoke release	1217:1235	Most importantly, the polyurethane composites filled with vermiculite filler and modified vermiculite fillers exhibited improved flame resistance characteristics (the value of total smoke release was reduced by ~34%, the value of peak heat release was reduced by ~25%).
34639165	4	25	theme	modified	709:716	arg1	composites					731:740	all the modified polyurethane composites	701:740	all the modified polyurethane composites	701:740	Among all the modified polyurethane composites, the greatest improvement was noticed in the PU foams filled with vermiculite modified with casein and chitosan.
34639165	3	26	theme	foaming	394:400	arg1	viscosity					420:428	dynamic viscosity	412:428	dynamic viscosity	412:428	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	26	theme	foaming	394:400	arg1	behavior					402:409	foaming behavior	394:409	foaming behavior	394:409	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	27	theme	fillers	306:312	arg1	impact					292:297	The impact	288:297	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength)	288:674	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	0	28	theme	Polyurethane	95:106	arg1	Foams					108:112	Polyurethane Foams	95:112	Polyurethane Foams	95:112	Vermiculite Filler Modified with Casein, Chitosan, and Potato Protein as a Flame Retardant for Polyurethane Foams.
34639165	3	29	theme	peak	553:556	arg1	release					558:564	heat peak release	548:564	heat peak release	548:564	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	29	theme	peak	553:556	arg1	index					526:530	limiting oxygen index	510:530	limiting oxygen index	510:530	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	6	30	theme	heat	1276:1279	arg1	release					1281:1287	peak heat release	1271:1287	peak heat release	1271:1287	Most importantly, the polyurethane composites filled with vermiculite filler and modified vermiculite fillers exhibited improved flame resistance characteristics (the value of total smoke release was reduced by ~34%, the value of peak heat release was reduced by ~25%).
34639165	3	31	theme	limiting	510:517	arg1	release					558:564	heat peak release	548:564	heat peak release	548:564	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	31	theme	limiting	510:517	arg1	index					526:530	limiting oxygen index	510:530	limiting oxygen index	510:530	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	31	theme	limiting	510:517	arg1	time					542:545	ignition time	533:545	ignition time	533:545	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	32	theme	obtained	344:351	arg1	composites					353:362	the obtained composites	340:362	the obtained composites	340:362	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	6	33	theme	peak	1271:1274	arg1	release					1281:1287	peak heat release	1271:1287	peak heat release	1271:1287	Most importantly, the polyurethane composites filled with vermiculite filler and modified vermiculite fillers exhibited improved flame resistance characteristics (the value of total smoke release was reduced by ~34%, the value of peak heat release was reduced by ~25%).
34639165	3	34	from	impact	292:297	arg1	thermal					432:438	thermal	432:438	thermal	432:438	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	34	from	impact	292:297	arg1	properties					583:592	mechanical properties	572:592	mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength)	572:674	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	34	from	impact	292:297	arg1	flame-retardant					487:501	flame-retardant	487:501	flame-retardant	487:501	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	34	from	impact	292:297	arg1	rheological					381:391	rheological	381:391	rheological	381:391	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	34	from	impact	292:297	arg1	properties					326:335	selected properties	317:335	selected properties	317:335	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	35	theme	oxygen	519:524	arg1	release					558:564	heat peak release	548:564	heat peak release	548:564	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	35	theme	oxygen	519:524	arg1	index					526:530	limiting oxygen index	510:530	limiting oxygen index	510:530	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	35	theme	oxygen	519:524	arg1	time					542:545	ignition time	533:545	ignition time	533:545	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	36	dep	strength	618:625	arg1	perpendicular					641:653	perpendicular	641:653	perpendicular	641:653	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	36	dep	strength	618:625	arg1	parallel					628:635	parallel	628:635	parallel	628:635	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	37	theme	heat	548:551	arg1	release					558:564	heat peak release	548:564	heat peak release	548:564	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	37	theme	heat	548:551	arg1	index					526:530	limiting oxygen index	510:530	limiting oxygen index	510:530	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	5	38	theme	flexural	982:989	arg1	strength					991:998	their flexural strength	976:998	their flexural strength by ~2-10%	976:1008	For example, after the addition of modified vermiculite fillers, the foams' compressive strength was enhanced by ~6-18%, their flexural strength by ~2-10%, and their toughness by ~1-5%.
34639165	3	39	dep	rheological	381:391	arg1	viscosity					420:428	dynamic viscosity	412:428	dynamic viscosity	412:428	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	39	dep	rheological	381:391	arg1	behavior					402:409	foaming behavior	394:409	foaming behavior	394:409	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	40	theme	flexural	657:664	arg1	toughness					595:603	toughness	595:603	toughness	595:603	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	40	theme	flexural	657:664	arg1	strength					666:673	flexural strength	657:673	flexural strength	657:673	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	4	41	theme	PU	787:788	arg1	foams					790:794	the PU foams	783:794	the PU foams filled with vermiculite modified with casein and chitosan	783:852	Among all the modified polyurethane composites, the greatest improvement was noticed in the PU foams filled with vermiculite modified with casein and chitosan.
34639165	4	42	theme	polyurethane	718:729	arg1	composites					731:740	all the modified polyurethane composites	701:740	all the modified polyurethane composites	701:740	Among all the modified polyurethane composites, the greatest improvement was noticed in the PU foams filled with vermiculite modified with casein and chitosan.
34639165	2	43	theme	potato	272:277	arg1	protein					279:285	potato protein	272:285	potato protein	272:285	% of vermiculite fillers, which were themselves modified with casein, chitosan, and potato protein.
34639165	3	44	theme	mechanical	572:581	arg1	properties					583:592	mechanical properties	572:592	mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength)	572:674	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	45	theme	ignition	533:540	arg1	index					526:530	limiting oxygen index	510:530	limiting oxygen index	510:530	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	45	theme	ignition	533:540	arg1	time					542:545	ignition time	533:545	ignition time	533:545	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	6	46	theme	resistance	1176:1185	arg1	characteristics					1187:1201	improved flame resistance characteristics	1161:1201	improved flame resistance characteristics	1161:1201	Most importantly, the polyurethane composites filled with vermiculite filler and modified vermiculite fillers exhibited improved flame resistance characteristics (the value of total smoke release was reduced by ~34%, the value of peak heat release was reduced by ~25%).
34639165	6	47	theme	polyurethane	1063:1074	arg1	composites					1076:1085	the polyurethane composites	1059:1085	the polyurethane composites filled with vermiculite filler and modified vermiculite fillers	1059:1149	Most importantly, the polyurethane composites filled with vermiculite filler and modified vermiculite fillers exhibited improved flame resistance characteristics (the value of total smoke release was reduced by ~34%, the value of peak heat release was reduced by ~25%).
34639165	2	48	theme	fillers	205:211	arg1	%					188:188	%	188:188	% of vermiculite fillers, which were themselves	188:234	% of vermiculite fillers, which were themselves modified with casein, chitosan, and potato protein.
34639165	2	48	theme	fillers	205:211	arg1	fillers					205:211	vermiculite fillers	193:211	vermiculite fillers	193:211	% of vermiculite fillers, which were themselves modified with casein, chitosan, and potato protein.
34639165	3	49	theme	dynamic	412:418	arg1	viscosity					420:428	dynamic viscosity	412:428	dynamic viscosity	412:428	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	3	49	theme	dynamic	412:418	arg1	behavior					402:409	foaming behavior	394:409	foaming behavior	394:409	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	0	50	theme	Potato	55:60	arg1	Protein					62:68	Potato Protein	55:68	Potato Protein	55:68	Vermiculite Filler Modified with Casein, Chitosan, and Potato Protein as a Flame Retardant for Polyurethane Foams.
34639165	2	51	theme	vermiculite	193:203	arg1	fillers					205:211	vermiculite fillers	193:211	vermiculite fillers	193:211	% of vermiculite fillers, which were themselves modified with casein, chitosan, and potato protein.
34639165	3	52	dep	index	526:530	arg1	e.g.					504:507	e.g.	504:507	e.g.	504:507	The impact of the fillers on selected properties of the obtained composites, including their rheological (foaming behavior, dynamic viscosity), thermal (temperature of thermal decomposition stages), flame-retardant (e.g., limiting oxygen index, ignition time, heat peak release), and mechanical properties (toughness, compressive strength (parallel and perpendicular), flexural strength) were investigated.
34639165	5	53	theme	vermiculite	899:909	arg1	fillers					911:917	modified vermiculite fillers	890:917	modified vermiculite fillers	890:917	For example, after the addition of modified vermiculite fillers, the foams' compressive strength was enhanced by ~6-18%, their flexural strength by ~2-10%, and their toughness by ~1-5%.
34639165	1	54	mod	modified	169:176	arg3	wt					185:186	2 wt	183:186	2 wt	183:186	In this study, polyurethane (PU) composite foams were modified with 2 wt.
34639165	1	54	mod	modified	169:176	arg1	foams					158:162	polyurethane (PU) composite foams	130:162	polyurethane (PU) composite foams	130:162	In this study, polyurethane (PU) composite foams were modified with 2 wt.
33381142	9	0	theme	HRGP	1644:1647	arg1	biosynthesis					1656:1667	HRGP glycan biosynthesis	1644:1667	HRGP glycan biosynthesis	1644:1667	These results reveal the diverse changes in HRGP-related processes that occur during wood formation at the gene expression and HRGP glycan biosynthesis levels, and relate HRGPs and glycosylation processes to the developmental processes of wood formation.
33381142	6	1	theme	ray	1097:1099	arg1	side					1106:1109	the ray cell side	1093:1109	the ray cell side of the vessel walls	1093:1129	In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits.
33381142	3	2	theme	data	491:494	arg1	Analysis					444:451	Analysis	444:451	Analysis of publicly available gene expression data	444:494	Analysis of publicly available gene expression data revealed that many HRGP encoding genes show tight spatio-temporal expression patterns in the developing wood of Populus that are indicative of specific functions during wood formation.
33381142	9	3	theme	biosynthesis	1656:1667	arg1	levels					1669:1674	the gene expression and HRGP glycan biosynthesis levels	1620:1674	the gene expression and HRGP glycan biosynthesis levels	1620:1674	These results reveal the diverse changes in HRGP-related processes that occur during wood formation at the gene expression and HRGP glycan biosynthesis levels, and relate HRGPs and glycosylation processes to the developmental processes of wood formation.
33381142	7	4	from	phloem/cambium	1220:1233	arg1	mass					1175:1178	Molecular mass and glycan analysis	1165:1198	mass	1175:1178	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	7	4	from	phloem/cambium	1220:1233	arg1	analysis					1191:1198	Molecular mass and glycan analysis	1165:1198	analysis	1191:1198	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	3	5	theme	expression	480:489	arg1	data					491:494	publicly available gene expression data	456:494	publicly available gene expression data	456:494	Analysis of publicly available gene expression data revealed that many HRGP encoding genes show tight spatio-temporal expression patterns in the developing wood of Populus that are indicative of specific functions during wood formation.
33381142	1	6	theme	associated	121:130	arg1	glycoproteins					152:164	Plant cell wall associated hydroxyproline-rich glycoproteins	105:164	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs)	105:172	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	1	6	theme	associated	121:130	arg1	HRGPs					167:171	HRGPs	167:171	HRGPs	167:171	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	3	7	theme	HRGP	515:518	arg1	genes					529:533	many HRGP encoding genes	510:533	many HRGP encoding genes	510:533	Analysis of publicly available gene expression data revealed that many HRGP encoding genes show tight spatio-temporal expression patterns in the developing wood of Populus that are indicative of specific functions during wood formation.
33381142	1	8	theme	several	190:196	arg1	aspects					198:204	several aspects	190:204	several aspects	190:204	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	1	8	theme	several	190:196	arg1	formation					254:262	wood formation	249:262	wood formation in trees	249:271	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	8	9	with	electrophoresis	1383:1397	arg1	Yariv					1430:1434	β-D-glucosyl Yariv	1417:1434	β-D-glucosyl Yariv	1417:1434	Separation of AGPs by agarose gel electrophoresis and staining with β-D-glucosyl Yariv confirmed the presence of different AGP populations in phloem/cambium and xylem.
33381142	3	10	theme	expression	562:571	arg1	patterns					573:580	tight spatio-temporal expression patterns	540:580	tight spatio-temporal expression patterns	540:580	Analysis of publicly available gene expression data revealed that many HRGP encoding genes show tight spatio-temporal expression patterns in the developing wood of Populus that are indicative of specific functions during wood formation.
33381142	5	11	theme	cell	892:895	arg1	specificity					902:912	the cell type specificity	888:912	the cell type specificity of different epitopes	888:934	In situ immunolabelling of transverse wood sections using AGP and EXT antibodies revealed the cell type specificity of different epitopes.
33381142	6	12	theme	xylem	981:985	arg1	walls					996:1000	xylem ray cell walls	981:1000	xylem ray cell walls	981:1000	In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits.
33381142	3	13	theme	tight	540:544	arg1	patterns					573:580	tight spatio-temporal expression patterns	540:580	tight spatio-temporal expression patterns	540:580	Analysis of publicly available gene expression data revealed that many HRGP encoding genes show tight spatio-temporal expression patterns in the developing wood of Populus that are indicative of specific functions during wood formation.
33381142	5	14	theme	AGP	856:858	arg1	antibodies					868:877	AGP and EXT antibodies	856:877	AGP and EXT antibodies	856:877	In situ immunolabelling of transverse wood sections using AGP and EXT antibodies revealed the cell type specificity of different epitopes.
33381142	1	15	gly	glycoproteins	152:164	arg1	glycoproteins					152:164	Plant cell wall associated hydroxyproline-rich glycoproteins	105:164	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs)	105:172	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	1	15	gly	glycoproteins	152:164	arg1	HRGPs					167:171	HRGPs	167:171	HRGPs	167:171	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	3	16	theme	developing	589:598	arg1	wood					600:603	the developing wood	585:603	the developing wood of Populus that are indicative of specific functions during wood formation	585:678	Analysis of publicly available gene expression data revealed that many HRGP encoding genes show tight spatio-temporal expression patterns in the developing wood of Populus that are indicative of specific functions during wood formation.
33381142	1	17	theme	development	226:236	arg1	aspects					198:204	several aspects	190:204	several aspects	190:204	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	1	17	theme	development	226:236	arg1	formation					254:262	wood formation	249:262	wood formation in trees	249:271	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	7	18	from	analysis	1191:1198	arg1	xylem					1265:1269	mature xylem	1258:1269	mature xylem	1258:1269	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	7	18	from	analysis	1191:1198	arg1	phloem/cambium					1220:1233	phloem/cambium	1220:1233	phloem/cambium	1220:1233	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	7	18	from	analysis	1191:1198	arg1	xylem					1247:1251	developing xylem	1236:1251	developing xylem	1236:1251	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	8	19	with	staining	1403:1410	arg1	Yariv					1430:1434	β-D-glucosyl Yariv	1417:1434	β-D-glucosyl Yariv	1417:1434	Separation of AGPs by agarose gel electrophoresis and staining with β-D-glucosyl Yariv confirmed the presence of different AGP populations in phloem/cambium and xylem.
33381142	1	20	from	formation	254:262	arg1	trees					267:271	trees	267:271	trees	267:271	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	1	21	theme	hydroxyproline-rich	132:150	arg1	glycoproteins					152:164	Plant cell wall associated hydroxyproline-rich glycoproteins	105:164	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs)	105:172	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	1	21	theme	hydroxyproline-rich	132:150	arg1	HRGPs					167:171	HRGPs	167:171	HRGPs	167:171	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	6	22	theme	wood	947:950	arg1	epitopes					956:963	mature wood AGP epitopes	940:963	mature wood AGP epitopes	940:963	In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits.
33381142	7	23	from	AGPs	1203:1206	arg1	xylem					1265:1269	mature xylem	1258:1269	mature xylem	1258:1269	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	7	23	from	AGPs	1203:1206	arg1	phloem/cambium					1220:1233	phloem/cambium	1220:1233	phloem/cambium	1220:1233	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	7	23	from	AGPs	1203:1206	arg1	xylem					1247:1251	developing xylem	1236:1251	developing xylem	1236:1251	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	9	24	theme	wood	1756:1759	arg1	formation					1761:1769	wood formation	1756:1769	wood formation	1756:1769	These results reveal the diverse changes in HRGP-related processes that occur during wood formation at the gene expression and HRGP glycan biosynthesis levels, and relate HRGPs and glycosylation processes to the developmental processes of wood formation.
33381142	7	25	from	EXTs	1212:1215	arg1	xylem					1265:1269	mature xylem	1258:1269	mature xylem	1258:1269	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	7	25	from	EXTs	1212:1215	arg1	phloem/cambium					1220:1233	phloem/cambium	1220:1233	phloem/cambium	1220:1233	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	7	25	from	EXTs	1212:1215	arg1	xylem					1247:1251	developing xylem	1236:1251	developing xylem	1236:1251	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	7	26	theme	clear	1280:1284	arg1	differences					1286:1296	clear differences	1280:1296	clear differences in glycan structures and size between the tissues	1280:1346	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	4	27	theme	Similar	681:687	arg1	results					689:695	Similar results	681:695	Similar results	681:695	Similar results were obtained for the expression of glycosyl transferases putatively involved in HRGP glycosylation.
33381142	7	28	theme	EXTs	1212:1215	arg1	mass					1175:1178	Molecular mass and glycan analysis	1165:1198	mass	1175:1178	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	7	28	theme	EXTs	1212:1215	arg1	analysis					1191:1198	Molecular mass and glycan analysis	1165:1198	analysis	1191:1198	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	9	29	from	changes	1550:1556	arg1	processes					1574:1582	HRGP-related processes	1561:1582	HRGP-related processes that occur during wood formation at the gene expression and HRGP glycan biosynthesis levels	1561:1674	These results reveal the diverse changes in HRGP-related processes that occur during wood formation at the gene expression and HRGP glycan biosynthesis levels, and relate HRGPs and glycosylation processes to the developmental processes of wood formation.
33381142	6	30	theme	EXT	1011:1013	arg1	epitopes					1015:1022	EXT epitopes	1011:1022	EXT epitopes	1011:1022	In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits.
33381142	7	31	theme	AGPs	1203:1206	arg1	mass					1175:1178	Molecular mass and glycan analysis	1165:1198	mass	1175:1178	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	7	31	theme	AGPs	1203:1206	arg1	analysis					1191:1198	Molecular mass and glycan analysis	1165:1198	analysis	1191:1198	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	5	32	theme	different	917:925	arg1	epitopes					927:934	different epitopes	917:934	different epitopes	917:934	In situ immunolabelling of transverse wood sections using AGP and EXT antibodies revealed the cell type specificity of different epitopes.
33381142	9	33	theme	diverse	1542:1548	arg1	changes					1550:1556	the diverse changes	1538:1556	the diverse changes in HRGP-related processes that occur during wood formation at the gene expression and HRGP glycan biosynthesis levels	1538:1674	These results reveal the diverse changes in HRGP-related processes that occur during wood formation at the gene expression and HRGP glycan biosynthesis levels, and relate HRGPs and glycosylation processes to the developmental processes of wood formation.
33381142	6	34	theme	cell	991:994	arg1	walls					996:1000	xylem ray cell walls	981:1000	xylem ray cell walls	981:1000	In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits.
33381142	6	35	from	walls	996:1000	arg1	located					970:976	located	970:976	located	970:976	In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits.
33381142	2	36	theme	plant	426:430	arg1	walls					437:441	plant cell walls	426:441	plant cell walls	426:441	HRGPs such as arabinogalactan-proteins (AGPs), extensins (EXTs), and proline rich proteins (PRPs) are important for the development and architecture of plant cell walls.
33381142	4	37	theme	glycosyl	733:740	arg1	transferases					742:753	glycosyl transferases	733:753	glycosyl transferases putatively involved in HRGP glycosylation	733:795	Similar results were obtained for the expression of glycosyl transferases putatively involved in HRGP glycosylation.
33381142	5	38	theme	transverse	825:834	arg1	sections					841:848	transverse wood sections	825:848	transverse wood sections using AGP and EXT antibodies	825:877	In situ immunolabelling of transverse wood sections using AGP and EXT antibodies revealed the cell type specificity of different epitopes.
33381142	8	39	theme	AGP	1472:1474	arg1	populations					1476:1486	different AGP populations	1462:1486	different AGP populations	1462:1486	Separation of AGPs by agarose gel electrophoresis and staining with β-D-glucosyl Yariv confirmed the presence of different AGP populations in phloem/cambium and xylem.
33381142	1	40	theme	Plant	105:109	arg1	glycoproteins					152:164	Plant cell wall associated hydroxyproline-rich glycoproteins	105:164	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs)	105:172	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	1	40	theme	Plant	105:109	arg1	HRGPs					167:171	HRGPs	167:171	HRGPs	167:171	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	7	41	theme	glycan	1301:1306	arg1	structures					1308:1317	glycan structures	1301:1317	glycan structures	1301:1317	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	8	42	theme	AGPs	1363:1366	arg1	Separation					1349:1358	Separation	1349:1358	Separation of AGPs by agarose gel electrophoresis and staining with β-D-glucosyl Yariv	1349:1434	Separation of AGPs by agarose gel electrophoresis and staining with β-D-glucosyl Yariv confirmed the presence of different AGP populations in phloem/cambium and xylem.
33381142	5	43	theme	sections	841:848	arg1	immunolabelling					806:820	In situ immunolabelling	798:820	In situ immunolabelling of transverse wood sections using AGP and EXT antibodies	798:877	In situ immunolabelling of transverse wood sections using AGP and EXT antibodies revealed the cell type specificity of different epitopes.
33381142	1	44	theme	wall	116:119	arg1	glycoproteins					152:164	Plant cell wall associated hydroxyproline-rich glycoproteins	105:164	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs)	105:172	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	1	44	theme	wall	116:119	arg1	HRGPs					167:171	HRGPs	167:171	HRGPs	167:171	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	6	45	theme	neighboring	1059:1069	arg1	vessels					1077:1083	neighboring xylem vessels	1059:1083	neighboring xylem vessels	1059:1083	In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits.
33381142	3	46	theme	specific	639:646	arg1	functions					648:656	specific functions	639:656	specific functions	639:656	Analysis of publicly available gene expression data revealed that many HRGP encoding genes show tight spatio-temporal expression patterns in the developing wood of Populus that are indicative of specific functions during wood formation.
33381142	8	47	theme	agarose	1371:1377	arg1	electrophoresis					1383:1397	agarose gel electrophoresis	1371:1397	agarose gel electrophoresis	1371:1397	Separation of AGPs by agarose gel electrophoresis and staining with β-D-glucosyl Yariv confirmed the presence of different AGP populations in phloem/cambium and xylem.
33381142	5	48	theme	In	798:799	arg1	immunolabelling					806:820	In situ immunolabelling	798:820	In situ immunolabelling of transverse wood sections using AGP and EXT antibodies	798:877	In situ immunolabelling of transverse wood sections using AGP and EXT antibodies revealed the cell type specificity of different epitopes.
33381142	0	49	theme	Cell	36:39	arg1	Glycoproteins					57:69	Cell Wall-Associated Glycoproteins	36:69	Cell Wall-Associated Glycoproteins	36:69	The Spatio-Temporal Distribution of Cell Wall-Associated Glycoproteins During Wood Formation in Populus.
33381142	9	50	theme	wood	1602:1605	arg1	formation					1607:1615	wood formation	1602:1615	wood formation	1602:1615	These results reveal the diverse changes in HRGP-related processes that occur during wood formation at the gene expression and HRGP glycan biosynthesis levels, and relate HRGPs and glycosylation processes to the developmental processes of wood formation.
33381142	7	51	from	xylem	1265:1269	arg1	mass					1175:1178	Molecular mass and glycan analysis	1165:1198	mass	1175:1178	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	7	51	from	xylem	1265:1269	arg1	analysis					1191:1198	Molecular mass and glycan analysis	1165:1198	analysis	1191:1198	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	2	52	theme	walls	437:441	arg1	architecture					410:421	architecture	410:421	architecture	410:421	HRGPs such as arabinogalactan-proteins (AGPs), extensins (EXTs), and proline rich proteins (PRPs) are important for the development and architecture of plant cell walls.
33381142	2	52	theme	walls	437:441	arg1	development					394:404	the development	390:404	the development	390:404	HRGPs such as arabinogalactan-proteins (AGPs), extensins (EXTs), and proline rich proteins (PRPs) are important for the development and architecture of plant cell walls.
33381142	0	53	theme	Wood	78:81	arg1	Formation					83:91	Wood Formation	78:91	Wood Formation in Populus	78:102	The Spatio-Temporal Distribution of Cell Wall-Associated Glycoproteins During Wood Formation in Populus.
33381142	3	54	theme	available	465:473	arg1	data					491:494	publicly available gene expression data	456:494	publicly available gene expression data	456:494	Analysis of publicly available gene expression data revealed that many HRGP encoding genes show tight spatio-temporal expression patterns in the developing wood of Populus that are indicative of specific functions during wood formation.
33381142	0	55	theme	Glycoproteins	57:69	arg1	Distribution					20:31	The Spatio-Temporal Distribution	0:31	The Spatio-Temporal Distribution of Cell Wall-Associated Glycoproteins During Wood Formation in Populus.	0:103	The Spatio-Temporal Distribution of Cell Wall-Associated Glycoproteins During Wood Formation in Populus.
33381142	6	56	theme	vessel	1118:1123	arg1	walls					1125:1129	the vessel walls	1114:1129	the vessel walls	1114:1129	In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits.
33381142	9	57	theme	gene	1624:1627	arg1	expression					1629:1638	gene expression	1624:1638	gene expression	1624:1638	These results reveal the diverse changes in HRGP-related processes that occur during wood formation at the gene expression and HRGP glycan biosynthesis levels, and relate HRGPs and glycosylation processes to the developmental processes of wood formation.
33381142	8	58	theme	β-D-glucosyl	1417:1428	arg1	Yariv					1430:1434	β-D-glucosyl Yariv	1417:1434	β-D-glucosyl Yariv	1417:1434	Separation of AGPs by agarose gel electrophoresis and staining with β-D-glucosyl Yariv confirmed the presence of different AGP populations in phloem/cambium and xylem.
33381142	8	59	attach	presence	1450:1457	arg2	populations					1476:1486	different AGP populations	1462:1486	different AGP populations	1462:1486	Separation of AGPs by agarose gel electrophoresis and staining with β-D-glucosyl Yariv confirmed the presence of different AGP populations in phloem/cambium and xylem.
33381142	8	59	attach	presence	1450:1457	arg1	xylem					1510:1514	xylem	1510:1514	xylem	1510:1514	Separation of AGPs by agarose gel electrophoresis and staining with β-D-glucosyl Yariv confirmed the presence of different AGP populations in phloem/cambium and xylem.
33381142	8	59	attach	presence	1450:1457	arg1	phloem/cambium					1491:1504	phloem/cambium	1491:1504	phloem/cambium	1491:1504	Separation of AGPs by agarose gel electrophoresis and staining with β-D-glucosyl Yariv confirmed the presence of different AGP populations in phloem/cambium and xylem.
33381142	1	60	theme	wood	249:252	arg1	formation					254:262	wood formation	249:262	wood formation in trees	249:271	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	6	61	theme	mature	940:945	arg1	epitopes					956:963	mature wood AGP epitopes	940:963	mature wood AGP epitopes	940:963	In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits.
33381142	6	62	theme	cell	1101:1104	arg1	side					1106:1109	the ray cell side	1093:1109	the ray cell side of the vessel walls	1093:1129	In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits.
33381142	2	63	theme	proline	343:349	arg1	PRPs					366:369	PRPs	366:369	PRPs	366:369	HRGPs such as arabinogalactan-proteins (AGPs), extensins (EXTs), and proline rich proteins (PRPs) are important for the development and architecture of plant cell walls.
33381142	2	63	theme	proline	343:349	arg1	proteins					356:363	proline rich proteins	343:363	proline rich proteins (PRPs)	343:370	HRGPs such as arabinogalactan-proteins (AGPs), extensins (EXTs), and proline rich proteins (PRPs) are important for the development and architecture of plant cell walls.
33381142	9	64	theme	glycan	1649:1654	arg1	biosynthesis					1656:1667	HRGP glycan biosynthesis	1644:1667	HRGP glycan biosynthesis	1644:1667	These results reveal the diverse changes in HRGP-related processes that occur during wood formation at the gene expression and HRGP glycan biosynthesis levels, and relate HRGPs and glycosylation processes to the developmental processes of wood formation.
33381142	3	65	theme	encoding	520:527	arg1	genes					529:533	many HRGP encoding genes	510:533	many HRGP encoding genes	510:533	Analysis of publicly available gene expression data revealed that many HRGP encoding genes show tight spatio-temporal expression patterns in the developing wood of Populus that are indicative of specific functions during wood formation.
33381142	6	66	theme	AGP	952:954	arg1	epitopes					956:963	mature wood AGP epitopes	940:963	mature wood AGP epitopes	940:963	In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits.
33381142	3	67	theme	many	510:513	arg1	genes					529:533	many HRGP encoding genes	510:533	many HRGP encoding genes	510:533	Analysis of publicly available gene expression data revealed that many HRGP encoding genes show tight spatio-temporal expression patterns in the developing wood of Populus that are indicative of specific functions during wood formation.
33381142	7	68	from	xylem	1247:1251	arg1	mass					1175:1178	Molecular mass and glycan analysis	1165:1198	mass	1175:1178	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	7	68	from	xylem	1247:1251	arg1	analysis					1191:1198	Molecular mass and glycan analysis	1165:1198	analysis	1191:1198	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	3	69	theme	spatio-temporal	546:560	arg1	patterns					573:580	tight spatio-temporal expression patterns	540:580	tight spatio-temporal expression patterns	540:580	Analysis of publicly available gene expression data revealed that many HRGP encoding genes show tight spatio-temporal expression patterns in the developing wood of Populus that are indicative of specific functions during wood formation.
33381142	6	70	theme	ray	987:989	arg1	walls					996:1000	xylem ray cell walls	981:1000	xylem ray cell walls	981:1000	In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits.
33381142	5	71	dep	In	798:799	arg1	situ					801:804	situ	801:804	situ	801:804	In situ immunolabelling of transverse wood sections using AGP and EXT antibodies revealed the cell type specificity of different epitopes.
33381142	1	72	theme	plant	209:213	arg1	growth					215:220	plant growth	209:220	plant growth	209:220	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	9	73	theme	glycosylation	1698:1710	arg1	processes					1712:1720	HRGPs and glycosylation processes	1688:1720	HRGPs and glycosylation processes	1688:1720	These results reveal the diverse changes in HRGP-related processes that occur during wood formation at the gene expression and HRGP glycan biosynthesis levels, and relate HRGPs and glycosylation processes to the developmental processes of wood formation.
33381142	7	74	from	mass	1175:1178	arg1	xylem					1265:1269	mature xylem	1258:1269	mature xylem	1258:1269	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	7	74	from	mass	1175:1178	arg1	phloem/cambium					1220:1233	phloem/cambium	1220:1233	phloem/cambium	1220:1233	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	7	74	from	mass	1175:1178	arg1	xylem					1247:1251	developing xylem	1236:1251	developing xylem	1236:1251	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	7	75	theme	mature	1258:1263	arg1	xylem					1265:1269	mature xylem	1258:1269	mature xylem	1258:1269	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	9	76	theme	HRGPs	1688:1692	arg1	processes					1712:1720	HRGPs and glycosylation processes	1688:1720	HRGPs and glycosylation processes	1688:1720	These results reveal the diverse changes in HRGP-related processes that occur during wood formation at the gene expression and HRGP glycan biosynthesis levels, and relate HRGPs and glycosylation processes to the developmental processes of wood formation.
33381142	0	77	from	Formation	83:91	arg1	Populus					96:102	Populus	96:102	Populus	96:102	The Spatio-Temporal Distribution of Cell Wall-Associated Glycoproteins During Wood Formation in Populus.
33381142	5	78	theme	epitopes	927:934	arg1	specificity					902:912	the cell type specificity	888:912	the cell type specificity of different epitopes	888:934	In situ immunolabelling of transverse wood sections using AGP and EXT antibodies revealed the cell type specificity of different epitopes.
33381142	6	79	with	association	1142:1152	arg1	pits					1159:1162	pits	1159:1162	pits	1159:1162	In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits.
33381142	9	80	theme	developmental	1729:1741	arg1	processes					1743:1751	the developmental processes	1725:1751	the developmental processes of wood formation	1725:1769	These results reveal the diverse changes in HRGP-related processes that occur during wood formation at the gene expression and HRGP glycan biosynthesis levels, and relate HRGPs and glycosylation processes to the developmental processes of wood formation.
33381142	8	81	theme	different	1462:1470	arg1	populations					1476:1486	different AGP populations	1462:1486	different AGP populations	1462:1486	Separation of AGPs by agarose gel electrophoresis and staining with β-D-glucosyl Yariv confirmed the presence of different AGP populations in phloem/cambium and xylem.
33381142	3	82	theme	Populus	608:614	arg1	wood					600:603	the developing wood	585:603	the developing wood of Populus that are indicative of specific functions during wood formation	585:678	Analysis of publicly available gene expression data revealed that many HRGP encoding genes show tight spatio-temporal expression patterns in the developing wood of Populus that are indicative of specific functions during wood formation.
33381142	8	83	theme	populations	1476:1486	arg1	presence					1450:1457	the presence	1446:1457	the presence of different AGP populations in phloem/cambium and xylem	1446:1514	Separation of AGPs by agarose gel electrophoresis and staining with β-D-glucosyl Yariv confirmed the presence of different AGP populations in phloem/cambium and xylem.
33381142	8	84	from	presence	1450:1457	arg1	xylem					1510:1514	xylem	1510:1514	xylem	1510:1514	Separation of AGPs by agarose gel electrophoresis and staining with β-D-glucosyl Yariv confirmed the presence of different AGP populations in phloem/cambium and xylem.
33381142	8	84	from	presence	1450:1457	arg1	phloem/cambium					1491:1504	phloem/cambium	1491:1504	phloem/cambium	1491:1504	Separation of AGPs by agarose gel electrophoresis and staining with β-D-glucosyl Yariv confirmed the presence of different AGP populations in phloem/cambium and xylem.
33381142	5	85	theme	type	897:900	arg1	specificity					902:912	the cell type specificity	888:912	the cell type specificity of different epitopes	888:934	In situ immunolabelling of transverse wood sections using AGP and EXT antibodies revealed the cell type specificity of different epitopes.
33381142	9	86	theme	formation	1761:1769	arg1	processes					1743:1751	the developmental processes	1725:1751	the developmental processes of wood formation	1725:1769	These results reveal the diverse changes in HRGP-related processes that occur during wood formation at the gene expression and HRGP glycan biosynthesis levels, and relate HRGPs and glycosylation processes to the developmental processes of wood formation.
33381142	6	87	located	observed	1042:1049	arg2	epitopes					1015:1022	EXT epitopes	1011:1022	EXT epitopes	1011:1022	In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits.
33381142	6	87	located	observed	1042:1049	arg1	association					1142:1152	association	1142:1152	association with pits	1142:1162	In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits.
33381142	7	88	theme	glycan	1184:1189	arg1	analysis					1191:1198	Molecular mass and glycan analysis	1165:1198	analysis	1191:1198	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	1	89	theme	growth	215:220	arg1	aspects					198:204	several aspects	190:204	several aspects	190:204	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	1	89	theme	growth	215:220	arg1	formation					254:262	wood formation	249:262	wood formation in trees	249:271	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	4	90	theme	HRGP	778:781	arg1	glycosylation					783:795	HRGP glycosylation	778:795	HRGP glycosylation	778:795	Similar results were obtained for the expression of glycosyl transferases putatively involved in HRGP glycosylation.
33381142	0	91	theme	Spatio-Temporal	4:18	arg1	Distribution					20:31	The Spatio-Temporal Distribution	0:31	The Spatio-Temporal Distribution of Cell Wall-Associated Glycoproteins During Wood Formation in Populus.	0:103	The Spatio-Temporal Distribution of Cell Wall-Associated Glycoproteins During Wood Formation in Populus.
33381142	9	92	theme	HRGP-related	1561:1572	arg1	processes					1574:1582	HRGP-related processes	1561:1582	HRGP-related processes that occur during wood formation at the gene expression and HRGP glycan biosynthesis levels	1561:1674	These results reveal the diverse changes in HRGP-related processes that occur during wood formation at the gene expression and HRGP glycan biosynthesis levels, and relate HRGPs and glycosylation processes to the developmental processes of wood formation.
33381142	5	93	theme	wood	836:839	arg1	sections					841:848	transverse wood sections	825:848	transverse wood sections using AGP and EXT antibodies	825:877	In situ immunolabelling of transverse wood sections using AGP and EXT antibodies revealed the cell type specificity of different epitopes.
33381142	1	94	theme	cell	111:114	arg1	glycoproteins					152:164	Plant cell wall associated hydroxyproline-rich glycoproteins	105:164	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs)	105:172	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	1	94	theme	cell	111:114	arg1	HRGPs					167:171	HRGPs	167:171	HRGPs	167:171	Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees.
33381142	6	95	theme	xylem	1071:1075	arg1	vessels					1077:1083	neighboring xylem vessels	1059:1083	neighboring xylem vessels	1059:1083	In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits.
33381142	6	96	from	located	970:976	arg1	epitopes					956:963	mature wood AGP epitopes	940:963	mature wood AGP epitopes	940:963	In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits.
33381142	6	96	from	located	970:976	arg1	walls					996:1000	xylem ray cell walls	981:1000	xylem ray cell walls	981:1000	In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits.
33381142	4	97	theme	transferases	742:753	arg1	expression					719:728	the expression	715:728	the expression of glycosyl transferases putatively involved in HRGP glycosylation	715:795	Similar results were obtained for the expression of glycosyl transferases putatively involved in HRGP glycosylation.
33381142	0	98	theme	Wall-Associated	41:55	arg1	Glycoproteins					57:69	Cell Wall-Associated Glycoproteins	36:69	Cell Wall-Associated Glycoproteins	36:69	The Spatio-Temporal Distribution of Cell Wall-Associated Glycoproteins During Wood Formation in Populus.
33381142	6	99	theme	walls	1125:1129	arg1	side					1106:1109	the ray cell side	1093:1109	the ray cell side of the vessel walls	1093:1129	In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits.
33381142	2	100	theme	cell	432:435	arg1	walls					437:441	plant cell walls	426:441	plant cell walls	426:441	HRGPs such as arabinogalactan-proteins (AGPs), extensins (EXTs), and proline rich proteins (PRPs) are important for the development and architecture of plant cell walls.
33381142	3	101	theme	wood	665:668	arg1	formation					670:678	wood formation	665:678	wood formation	665:678	Analysis of publicly available gene expression data revealed that many HRGP encoding genes show tight spatio-temporal expression patterns in the developing wood of Populus that are indicative of specific functions during wood formation.
33381142	8	102	theme	gel	1379:1381	arg1	electrophoresis					1383:1397	agarose gel electrophoresis	1371:1397	agarose gel electrophoresis	1371:1397	Separation of AGPs by agarose gel electrophoresis and staining with β-D-glucosyl Yariv confirmed the presence of different AGP populations in phloem/cambium and xylem.
33381142	3	103	theme	gene	475:478	arg1	data					491:494	publicly available gene expression data	456:494	publicly available gene expression data	456:494	Analysis of publicly available gene expression data revealed that many HRGP encoding genes show tight spatio-temporal expression patterns in the developing wood of Populus that are indicative of specific functions during wood formation.
33381142	7	104	from	differences	1286:1296	arg1	size					1323:1326	size	1323:1326	size	1323:1326	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	7	104	from	differences	1286:1296	arg1	structures					1308:1317	glycan structures	1301:1317	glycan structures	1301:1317	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	6	105	from	epitopes	956:963	arg1	located					970:976	located	970:976	located	970:976	In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits.
33381142	3	106	theme	functions	648:656	arg1	indicative					625:634	indicative	625:634	indicative	625:634	Analysis of publicly available gene expression data revealed that many HRGP encoding genes show tight spatio-temporal expression patterns in the developing wood of Populus that are indicative of specific functions during wood formation.
33381142	7	107	theme	developing	1236:1245	arg1	xylem					1247:1251	developing xylem	1236:1251	developing xylem	1236:1251	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
33381142	5	108	theme	EXT	864:866	arg1	antibodies					868:877	AGP and EXT antibodies	856:877	AGP and EXT antibodies	856:877	In situ immunolabelling of transverse wood sections using AGP and EXT antibodies revealed the cell type specificity of different epitopes.
33381142	9	109	theme	expression	1629:1638	arg1	levels					1669:1674	the gene expression and HRGP glycan biosynthesis levels	1620:1674	the gene expression and HRGP glycan biosynthesis levels	1620:1674	These results reveal the diverse changes in HRGP-related processes that occur during wood formation at the gene expression and HRGP glycan biosynthesis levels, and relate HRGPs and glycosylation processes to the developmental processes of wood formation.
33381142	2	110	theme	rich	351:354	arg1	PRPs					366:369	PRPs	366:369	PRPs	366:369	HRGPs such as arabinogalactan-proteins (AGPs), extensins (EXTs), and proline rich proteins (PRPs) are important for the development and architecture of plant cell walls.
33381142	2	110	theme	rich	351:354	arg1	proteins					356:363	proline rich proteins	343:363	proline rich proteins (PRPs)	343:370	HRGPs such as arabinogalactan-proteins (AGPs), extensins (EXTs), and proline rich proteins (PRPs) are important for the development and architecture of plant cell walls.
33381142	7	111	theme	Molecular	1165:1173	arg1	mass					1175:1178	Molecular mass and glycan analysis	1165:1198	mass	1175:1178	Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues.
32800955	7	0	theme	Zn-based	1022:1029	arg1	paint					1043:1047	Zn-based antifouling paint	1022:1047	Zn-based antifouling paint in the experiments with B. subtilis and E. coli	1022:1095	CHT/ZnO composite coated fiberglass panels showed the strongest antimicrobial activity compared to chitosan, ZnO NRs coatings, and Zn-based antifouling paint in the experiments with B. subtilis and E. coli under light conditions.
32800955	9	1	theme	good	1220:1223	arg1	alternatives					1225:1236	good alternatives	1220:1236	good alternatives to the toxic antifouling paints	1220:1268	CHT/ZnO composites can be good alternatives to the toxic antifouling paints.
32800955	4	2	dep	ZnO	546:548	arg1	the					542:544	the	542:544	the	542:544	XRD and FTIR results showed the presence of the ZnO and chitosan.
32800955	7	3	theme	strongest	945:953	arg1	activity					969:976	the strongest antimicrobial activity	941:976	the strongest antimicrobial activity	941:976	CHT/ZnO composite coated fiberglass panels showed the strongest antimicrobial activity compared to chitosan, ZnO NRs coatings, and Zn-based antifouling paint in the experiments with B. subtilis and E. coli under light conditions.
32800955	2	4	with	microrods	280:288	arg1	size					240:243	size	240:243	size ranging from 20 to 100 nm	240:269	ZnO nanorods (NRs) with size ranging from 20 to 100 nm and some microrods with an approximate size of 0.5-1 μm were grown on fiberglass panels.
32800955	2	4	with	microrods	280:288	arg1	size					310:313	an approximate size	295:313	an approximate size of 0.5-1 μm	295:325	ZnO nanorods (NRs) with size ranging from 20 to 100 nm and some microrods with an approximate size of 0.5-1 μm were grown on fiberglass panels.
32800955	6	5	theme	biofilm-forming	789:803	arg1	bacteria					805:812	biofilm-forming bacteria Bacillus subtilis and Escherichia coli	789:851	biofilm-forming bacteria Bacillus subtilis and Escherichia coli	789:851	The antimicrobial activities of the coated fiberglass panels were investigated using biofilm-forming bacteria Bacillus subtilis and Escherichia coli under both light and dark conditions.
32800955	7	6	theme	antifouling	1031:1041	arg1	paint					1043:1047	Zn-based antifouling paint	1022:1047	Zn-based antifouling paint in the experiments with B. subtilis and E. coli	1022:1095	CHT/ZnO composite coated fiberglass panels showed the strongest antimicrobial activity compared to chitosan, ZnO NRs coatings, and Zn-based antifouling paint in the experiments with B. subtilis and E. coli under light conditions.
32800955	5	7	theme	CHT	669:671	arg1	NRs					680:682	CHT 2%/ZnO NRs	669:682	CHT 2%/ZnO NRs	669:682	The water contact angle decreased from 80° ± 2° (control) to 65° ± 2° for CHT 1%/ZnO NRs and 42 ± 2° for CHT 2%/ZnO NRs composite coatings.
32800955	6	8	theme	dark	874:877	arg1	conditions					879:888	both light and dark conditions	859:888	both light and dark conditions	859:888	The antimicrobial activities of the coated fiberglass panels were investigated using biofilm-forming bacteria Bacillus subtilis and Escherichia coli under both light and dark conditions.
32800955	4	9	theme	XRD	498:500	arg1	results					511:517	XRD and FTIR results	498:517	XRD and FTIR results	498:517	XRD and FTIR results showed the presence of the ZnO and chitosan.
32800955	7	10	dep	showed	934:939	arg1	compared					978:985	compared	978:985	showed the strongest antimicrobial activity compared to chitosan, ZnO NRs coatings, and Zn-based antifouling paint in the experiments with B. subtilis and E. coli under light conditions	934:1118	CHT/ZnO composite coated fiberglass panels showed the strongest antimicrobial activity compared to chitosan, ZnO NRs coatings, and Zn-based antifouling paint in the experiments with B. subtilis and E. coli under light conditions.
32800955	9	11	theme	CHT/ZnO	1194:1200	arg1	composites					1202:1211	CHT/ZnO composites	1194:1211	CHT/ZnO composites	1194:1211	CHT/ZnO composites can be good alternatives to the toxic antifouling paints.
32800955	5	12	theme	water	568:572	arg1	angle					582:586	The water contact angle	564:586	The water contact angle	564:586	The water contact angle decreased from 80° ± 2° (control) to 65° ± 2° for CHT 1%/ZnO NRs and 42 ± 2° for CHT 2%/ZnO NRs composite coatings.
32800955	3	13	from	visible	457:463	arg1	NRs					483:485	the CHT 2%/ZnO NRs	468:485	the CHT 2%/ZnO NRs composite	468:495	CHT 1%/ZnO composite had ZnO NRs incorporated into chitosan (CHT) coating while ZnO NRs were not visible in the CHT 2%/ZnO NRs composite.
32800955	3	14	theme	2	476:476	arg1	%					477:477	%	477:477	%	477:477	CHT 1%/ZnO composite had ZnO NRs incorporated into chitosan (CHT) coating while ZnO NRs were not visible in the CHT 2%/ZnO NRs composite.
32800955	7	15	theme	light	1103:1107	arg1	conditions					1109:1118	light conditions	1103:1118	light conditions	1103:1118	CHT/ZnO composite coated fiberglass panels showed the strongest antimicrobial activity compared to chitosan, ZnO NRs coatings, and Zn-based antifouling paint in the experiments with B. subtilis and E. coli under light conditions.
32800955	5	16	theme	contact	574:580	arg1	angle					582:586	The water contact angle	564:586	The water contact angle	564:586	The water contact angle decreased from 80° ± 2° (control) to 65° ± 2° for CHT 1%/ZnO NRs and 42 ± 2° for CHT 2%/ZnO NRs composite coatings.
32800955	3	17	contain	had	381:383	arg1	composite					371:379	CHT 1%/ZnO composite	360:379	CHT 1%/ZnO composite	360:379	CHT 1%/ZnO composite had ZnO NRs incorporated into chitosan (CHT) coating while ZnO NRs were not visible in the CHT 2%/ZnO NRs composite.
32800955	3	17	contain	had	381:383	arg2	NRs					389:391	ZnO NRs	385:391	ZnO NRs incorporated into chitosan (CHT) coating while ZnO NRs were not visible in the CHT 2%/ZnO NRs composite	385:495	CHT 1%/ZnO composite had ZnO NRs incorporated into chitosan (CHT) coating while ZnO NRs were not visible in the CHT 2%/ZnO NRs composite.
32800955	3	18	theme	%	477:477	arg1	NRs					483:485	the CHT 2%/ZnO NRs	468:485	the CHT 2%/ZnO NRs composite	468:495	CHT 1%/ZnO composite had ZnO NRs incorporated into chitosan (CHT) coating while ZnO NRs were not visible in the CHT 2%/ZnO NRs composite.
32800955	1	19	theme	composite	154:162	arg1	fabrication					110:120	the fabrication	106:120	the fabrication of chitosan‑zinc oxide nanorods composite (CHT/ZnO) on fiberglass panels (support substrate)	106:213	The current study describes the fabrication of chitosan‑zinc oxide nanorods composite (CHT/ZnO) on fiberglass panels (support substrate).
32800955	5	20	theme	CHT	638:640	arg1	NRs					649:651	CHT 1%/ZnO NRs	638:651	CHT 1%/ZnO NRs	638:651	The water contact angle decreased from 80° ± 2° (control) to 65° ± 2° for CHT 1%/ZnO NRs and 42 ± 2° for CHT 2%/ZnO NRs composite coatings.
32800955	1	21	theme	chitosan‑zinc	125:137	arg1	CHT/ZnO					165:171	CHT/ZnO	165:171	CHT/ZnO	165:171	The current study describes the fabrication of chitosan‑zinc oxide nanorods composite (CHT/ZnO) on fiberglass panels (support substrate).
32800955	1	21	theme	chitosan‑zinc	125:137	arg1	composite					154:162	chitosan‑zinc oxide nanorods composite	125:162	chitosan‑zinc oxide nanorods composite (CHT/ZnO)	125:172	The current study describes the fabrication of chitosan‑zinc oxide nanorods composite (CHT/ZnO) on fiberglass panels (support substrate).
32800955	7	22	theme	NRs	1004:1006	arg1	coatings					1008:1015	ZnO NRs coatings	1000:1015	ZnO NRs coatings	1000:1015	CHT/ZnO composite coated fiberglass panels showed the strongest antimicrobial activity compared to chitosan, ZnO NRs coatings, and Zn-based antifouling paint in the experiments with B. subtilis and E. coli under light conditions.
32800955	5	23	theme	%	674:674	arg1	NRs					680:682	CHT 2%/ZnO NRs	669:682	CHT 2%/ZnO NRs	669:682	The water contact angle decreased from 80° ± 2° (control) to 65° ± 2° for CHT 1%/ZnO NRs and 42 ± 2° for CHT 2%/ZnO NRs composite coatings.
32800955	1	24	theme	current	82:88	arg1	study					90:94	The current study	78:94	The current study	78:94	The current study describes the fabrication of chitosan‑zinc oxide nanorods composite (CHT/ZnO) on fiberglass panels (support substrate).
32800955	1	25	theme	fiberglass	177:186	arg1	panels					188:193	fiberglass panels	177:193	fiberglass panels (support substrate)	177:213	The current study describes the fabrication of chitosan‑zinc oxide nanorods composite (CHT/ZnO) on fiberglass panels (support substrate).
32800955	1	25	theme	fiberglass	177:186	arg1	substrate					204:212	support substrate	196:212	support substrate	196:212	The current study describes the fabrication of chitosan‑zinc oxide nanorods composite (CHT/ZnO) on fiberglass panels (support substrate).
32800955	3	26	theme	/ZnO	366:369	arg1	composite					371:379	CHT 1%/ZnO composite	360:379	CHT 1%/ZnO composite	360:379	CHT 1%/ZnO composite had ZnO NRs incorporated into chitosan (CHT) coating while ZnO NRs were not visible in the CHT 2%/ZnO NRs composite.
32800955	6	27	theme	panels	758:763	arg1	activities					722:731	The antimicrobial activities	704:731	The antimicrobial activities of the coated fiberglass panels	704:763	The antimicrobial activities of the coated fiberglass panels were investigated using biofilm-forming bacteria Bacillus subtilis and Escherichia coli under both light and dark conditions.
32800955	3	28	theme	CHT	472:474	arg1	NRs					483:485	the CHT 2%/ZnO NRs	468:485	the CHT 2%/ZnO NRs composite	468:495	CHT 1%/ZnO composite had ZnO NRs incorporated into chitosan (CHT) coating while ZnO NRs were not visible in the CHT 2%/ZnO NRs composite.
32800955	7	29	from	chitosan	990:997	arg1	experiments					1056:1066	the experiments	1052:1066	the experiments with B. subtilis and E. coli	1052:1095	CHT/ZnO composite coated fiberglass panels showed the strongest antimicrobial activity compared to chitosan, ZnO NRs coatings, and Zn-based antifouling paint in the experiments with B. subtilis and E. coli under light conditions.
32800955	8	30	theme	antifouling	1133:1143	arg1	activity					1145:1152	The highest antifouling activity	1121:1152	The highest antifouling activity	1121:1152	The highest antifouling activity was observed for CHT 2%/ZnO composites.
32800955	3	31	theme	CHT	360:362	arg1	composite					371:379	CHT 1%/ZnO composite	360:379	CHT 1%/ZnO composite	360:379	CHT 1%/ZnO composite had ZnO NRs incorporated into chitosan (CHT) coating while ZnO NRs were not visible in the CHT 2%/ZnO NRs composite.
32800955	6	32	theme	fiberglass	747:756	arg1	panels					758:763	the coated fiberglass panels	736:763	the coated fiberglass panels	736:763	The antimicrobial activities of the coated fiberglass panels were investigated using biofilm-forming bacteria Bacillus subtilis and Escherichia coli under both light and dark conditions.
32800955	3	33	theme	ZnO	385:387	arg1	NRs					389:391	ZnO NRs	385:391	ZnO NRs incorporated into chitosan (CHT) coating while ZnO NRs were not visible in the CHT 2%/ZnO NRs composite	385:495	CHT 1%/ZnO composite had ZnO NRs incorporated into chitosan (CHT) coating while ZnO NRs were not visible in the CHT 2%/ZnO NRs composite.
32800955	3	34	theme	1	364:364	arg1	%					365:365	%	365:365	%	365:365	CHT 1%/ZnO composite had ZnO NRs incorporated into chitosan (CHT) coating while ZnO NRs were not visible in the CHT 2%/ZnO NRs composite.
32800955	6	35	dep	bacteria	805:812	arg1	subtilis					823:830	Bacillus subtilis	814:830	Bacillus subtilis	814:830	The antimicrobial activities of the coated fiberglass panels were investigated using biofilm-forming bacteria Bacillus subtilis and Escherichia coli under both light and dark conditions.
32800955	6	35	dep	bacteria	805:812	arg1	coli					848:851	Escherichia coli	836:851	Escherichia coli	836:851	The antimicrobial activities of the coated fiberglass panels were investigated using biofilm-forming bacteria Bacillus subtilis and Escherichia coli under both light and dark conditions.
32800955	5	36	theme	/ZnO	675:678	arg1	NRs					680:682	CHT 2%/ZnO NRs	669:682	CHT 2%/ZnO NRs	669:682	The water contact angle decreased from 80° ± 2° (control) to 65° ± 2° for CHT 1%/ZnO NRs and 42 ± 2° for CHT 2%/ZnO NRs composite coatings.
32800955	1	37	theme	support	196:202	arg1	panels					188:193	fiberglass panels	177:193	fiberglass panels (support substrate)	177:213	The current study describes the fabrication of chitosan‑zinc oxide nanorods composite (CHT/ZnO) on fiberglass panels (support substrate).
32800955	1	37	theme	support	196:202	arg1	substrate					204:212	support substrate	196:212	support substrate	196:212	The current study describes the fabrication of chitosan‑zinc oxide nanorods composite (CHT/ZnO) on fiberglass panels (support substrate).
32800955	2	38	with	nanorods	220:227	arg1	size					240:243	size	240:243	size ranging from 20 to 100 nm	240:269	ZnO nanorods (NRs) with size ranging from 20 to 100 nm and some microrods with an approximate size of 0.5-1 μm were grown on fiberglass panels.
32800955	2	38	with	nanorods	220:227	arg1	size					310:313	an approximate size	295:313	an approximate size of 0.5-1 μm	295:325	ZnO nanorods (NRs) with size ranging from 20 to 100 nm and some microrods with an approximate size of 0.5-1 μm were grown on fiberglass panels.
32800955	3	39	theme	/ZnO	478:481	arg1	NRs					483:485	the CHT 2%/ZnO NRs	468:485	the CHT 2%/ZnO NRs composite	468:495	CHT 1%/ZnO composite had ZnO NRs incorporated into chitosan (CHT) coating while ZnO NRs were not visible in the CHT 2%/ZnO NRs composite.
32800955	4	40	theme	ZnO	546:548	arg1	presence					530:537	the presence	526:537	the presence of the ZnO and chitosan	526:561	XRD and FTIR results showed the presence of the ZnO and chitosan.
32800955	0	41	theme	composite	21:29	arg1	coatings					31:38	composite coatings	21:38	composite coatings with enhanced antifouling properties	21:75	ZnO nanorod-chitosan composite coatings with enhanced antifouling properties.
32800955	6	42	theme	antimicrobial	708:720	arg1	activities					722:731	The antimicrobial activities	704:731	The antimicrobial activities of the coated fiberglass panels	704:763	The antimicrobial activities of the coated fiberglass panels were investigated using biofilm-forming bacteria Bacillus subtilis and Escherichia coli under both light and dark conditions.
32800955	8	43	theme	CHT	1171:1173	arg1	composites					1182:1191	CHT 2%/ZnO composites	1171:1191	CHT 2%/ZnO composites	1171:1191	The highest antifouling activity was observed for CHT 2%/ZnO composites.
32800955	6	44	theme	coated	740:745	arg1	panels					758:763	the coated fiberglass panels	736:763	the coated fiberglass panels	736:763	The antimicrobial activities of the coated fiberglass panels were investigated using biofilm-forming bacteria Bacillus subtilis and Escherichia coli under both light and dark conditions.
32800955	0	45	theme	enhanced	45:52	arg1	properties					66:75	enhanced antifouling properties	45:75	enhanced antifouling properties	45:75	ZnO nanorod-chitosan composite coatings with enhanced antifouling properties.
32800955	7	46	from	coatings	1008:1015	arg1	experiments					1056:1066	the experiments	1052:1066	the experiments with B. subtilis and E. coli	1052:1095	CHT/ZnO composite coated fiberglass panels showed the strongest antimicrobial activity compared to chitosan, ZnO NRs coatings, and Zn-based antifouling paint in the experiments with B. subtilis and E. coli under light conditions.
32800955	8	47	theme	%	1176:1176	arg1	composites					1182:1191	CHT 2%/ZnO composites	1171:1191	CHT 2%/ZnO composites	1171:1191	The highest antifouling activity was observed for CHT 2%/ZnO composites.
32800955	4	48	theme	chitosan	554:561	arg1	presence					530:537	the presence	526:537	the presence of the ZnO and chitosan	526:561	XRD and FTIR results showed the presence of the ZnO and chitosan.
32800955	9	49	theme	antifouling	1251:1261	arg1	paints					1263:1268	the toxic antifouling paints	1241:1268	the toxic antifouling paints	1241:1268	CHT/ZnO composites can be good alternatives to the toxic antifouling paints.
32800955	0	50	with	coatings	31:38	arg1	properties					66:75	enhanced antifouling properties	45:75	enhanced antifouling properties	45:75	ZnO nanorod-chitosan composite coatings with enhanced antifouling properties.
32800955	5	51	theme	1	642:642	arg1	%					643:643	%	643:643	%	643:643	The water contact angle decreased from 80° ± 2° (control) to 65° ± 2° for CHT 1%/ZnO NRs and 42 ± 2° for CHT 2%/ZnO NRs composite coatings.
32800955	3	52	theme	%	365:365	arg1	composite					371:379	CHT 1%/ZnO composite	360:379	CHT 1%/ZnO composite	360:379	CHT 1%/ZnO composite had ZnO NRs incorporated into chitosan (CHT) coating while ZnO NRs were not visible in the CHT 2%/ZnO NRs composite.
32800955	5	53	theme	2	673:673	arg1	%					674:674	%	674:674	%	674:674	The water contact angle decreased from 80° ± 2° (control) to 65° ± 2° for CHT 1%/ZnO NRs and 42 ± 2° for CHT 2%/ZnO NRs composite coatings.
32800955	6	54	theme	light	864:868	arg1	conditions					879:888	both light and dark conditions	859:888	both light and dark conditions	859:888	The antimicrobial activities of the coated fiberglass panels were investigated using biofilm-forming bacteria Bacillus subtilis and Escherichia coli under both light and dark conditions.
32800955	2	55	theme	0.5-1 μm	318:325	arg1	size					310:313	an approximate size	295:313	an approximate size of 0.5-1 μm	295:325	ZnO nanorods (NRs) with size ranging from 20 to 100 nm and some microrods with an approximate size of 0.5-1 μm were grown on fiberglass panels.
32800955	3	56	theme	ZnO	440:442	arg1	NRs					444:446	ZnO NRs	440:446	ZnO NRs	440:446	CHT 1%/ZnO composite had ZnO NRs incorporated into chitosan (CHT) coating while ZnO NRs were not visible in the CHT 2%/ZnO NRs composite.
32800955	0	57	theme	antifouling	54:64	arg1	properties					66:75	enhanced antifouling properties	45:75	enhanced antifouling properties	45:75	ZnO nanorod-chitosan composite coatings with enhanced antifouling properties.
32800955	7	58	theme	antimicrobial	955:967	arg1	activity					969:976	the strongest antimicrobial activity	941:976	the strongest antimicrobial activity	941:976	CHT/ZnO composite coated fiberglass panels showed the strongest antimicrobial activity compared to chitosan, ZnO NRs coatings, and Zn-based antifouling paint in the experiments with B. subtilis and E. coli under light conditions.
32800955	2	59	theme	ZnO	216:218	arg1	NRs					230:232	NRs	230:232	NRs	230:232	ZnO nanorods (NRs) with size ranging from 20 to 100 nm and some microrods with an approximate size of 0.5-1 μm were grown on fiberglass panels.
32800955	2	59	theme	ZnO	216:218	arg1	nanorods					220:227	ZnO nanorods	216:227	ZnO nanorods (NRs) with size ranging from 20 to 100 nm	216:269	ZnO nanorods (NRs) with size ranging from 20 to 100 nm and some microrods with an approximate size of 0.5-1 μm were grown on fiberglass panels.
32800955	7	60	theme	composite	899:907	arg1	panels					927:932	CHT/ZnO composite coated fiberglass panels	891:932	CHT/ZnO composite coated fiberglass panels	891:932	CHT/ZnO composite coated fiberglass panels showed the strongest antimicrobial activity compared to chitosan, ZnO NRs coatings, and Zn-based antifouling paint in the experiments with B. subtilis and E. coli under light conditions.
32800955	1	61	theme	oxide	139:143	arg1	CHT/ZnO					165:171	CHT/ZnO	165:171	CHT/ZnO	165:171	The current study describes the fabrication of chitosan‑zinc oxide nanorods composite (CHT/ZnO) on fiberglass panels (support substrate).
32800955	1	61	theme	oxide	139:143	arg1	composite					154:162	chitosan‑zinc oxide nanorods composite	125:162	chitosan‑zinc oxide nanorods composite (CHT/ZnO)	125:172	The current study describes the fabrication of chitosan‑zinc oxide nanorods composite (CHT/ZnO) on fiberglass panels (support substrate).
32800955	1	62	from	fabrication	110:120	arg1	panels					188:193	fiberglass panels	177:193	fiberglass panels (support substrate)	177:213	The current study describes the fabrication of chitosan‑zinc oxide nanorods composite (CHT/ZnO) on fiberglass panels (support substrate).
32800955	1	62	from	fabrication	110:120	arg1	substrate					204:212	support substrate	196:212	support substrate	196:212	The current study describes the fabrication of chitosan‑zinc oxide nanorods composite (CHT/ZnO) on fiberglass panels (support substrate).
32800955	5	63	dep	65° ± 2°	625:632	arg1	coatings					694:701	composite coatings	684:701	composite coatings	684:701	The water contact angle decreased from 80° ± 2° (control) to 65° ± 2° for CHT 1%/ZnO NRs and 42 ± 2° for CHT 2%/ZnO NRs composite coatings.
32800955	9	64	theme	toxic	1245:1249	arg1	paints					1263:1268	the toxic antifouling paints	1241:1268	the toxic antifouling paints	1241:1268	CHT/ZnO composites can be good alternatives to the toxic antifouling paints.
32800955	7	65	theme	coated	909:914	arg1	panels					927:932	CHT/ZnO composite coated fiberglass panels	891:932	CHT/ZnO composite coated fiberglass panels	891:932	CHT/ZnO composite coated fiberglass panels showed the strongest antimicrobial activity compared to chitosan, ZnO NRs coatings, and Zn-based antifouling paint in the experiments with B. subtilis and E. coli under light conditions.
32800955	1	66	theme	nanorods	145:152	arg1	CHT/ZnO					165:171	CHT/ZnO	165:171	CHT/ZnO	165:171	The current study describes the fabrication of chitosan‑zinc oxide nanorods composite (CHT/ZnO) on fiberglass panels (support substrate).
32800955	1	66	theme	nanorods	145:152	arg1	composite					154:162	chitosan‑zinc oxide nanorods composite	125:162	chitosan‑zinc oxide nanorods composite (CHT/ZnO)	125:172	The current study describes the fabrication of chitosan‑zinc oxide nanorods composite (CHT/ZnO) on fiberglass panels (support substrate).
32800955	3	67	from	NRs	483:485	arg1	visible					457:463	visible	457:463	visible	457:463	CHT 1%/ZnO composite had ZnO NRs incorporated into chitosan (CHT) coating while ZnO NRs were not visible in the CHT 2%/ZnO NRs composite.
32800955	7	68	with	experiments	1056:1066	arg1	subtilis					1076:1083	B. subtilis	1073:1083	B. subtilis	1073:1083	CHT/ZnO composite coated fiberglass panels showed the strongest antimicrobial activity compared to chitosan, ZnO NRs coatings, and Zn-based antifouling paint in the experiments with B. subtilis and E. coli under light conditions.
32800955	7	68	with	experiments	1056:1066	arg1	coli					1092:1095	E. coli	1089:1095	E. coli	1089:1095	CHT/ZnO composite coated fiberglass panels showed the strongest antimicrobial activity compared to chitosan, ZnO NRs coatings, and Zn-based antifouling paint in the experiments with B. subtilis and E. coli under light conditions.
32800955	7	69	theme	fiberglass	916:925	arg1	panels					927:932	CHT/ZnO composite coated fiberglass panels	891:932	CHT/ZnO composite coated fiberglass panels	891:932	CHT/ZnO composite coated fiberglass panels showed the strongest antimicrobial activity compared to chitosan, ZnO NRs coatings, and Zn-based antifouling paint in the experiments with B. subtilis and E. coli under light conditions.
32800955	2	70	theme	approximate	298:308	arg1	size					310:313	an approximate size	295:313	an approximate size of 0.5-1 μm	295:325	ZnO nanorods (NRs) with size ranging from 20 to 100 nm and some microrods with an approximate size of 0.5-1 μm were grown on fiberglass panels.
32800955	8	71	theme	highest	1125:1131	arg1	activity					1145:1152	The highest antifouling activity	1121:1152	The highest antifouling activity	1121:1152	The highest antifouling activity was observed for CHT 2%/ZnO composites.
32800955	5	72	theme	%	643:643	arg1	NRs					649:651	CHT 1%/ZnO NRs	638:651	CHT 1%/ZnO NRs	638:651	The water contact angle decreased from 80° ± 2° (control) to 65° ± 2° for CHT 1%/ZnO NRs and 42 ± 2° for CHT 2%/ZnO NRs composite coatings.
32800955	8	73	theme	2	1175:1175	arg1	%					1176:1176	%	1176:1176	%	1176:1176	The highest antifouling activity was observed for CHT 2%/ZnO composites.
32800955	7	74	theme	CHT/ZnO	891:897	arg1	panels					927:932	CHT/ZnO composite coated fiberglass panels	891:932	CHT/ZnO composite coated fiberglass panels	891:932	CHT/ZnO composite coated fiberglass panels showed the strongest antimicrobial activity compared to chitosan, ZnO NRs coatings, and Zn-based antifouling paint in the experiments with B. subtilis and E. coli under light conditions.
32800955	2	75	theme	fiberglass	341:350	arg1	panels					352:357	fiberglass panels	341:357	fiberglass panels	341:357	ZnO nanorods (NRs) with size ranging from 20 to 100 nm and some microrods with an approximate size of 0.5-1 μm were grown on fiberglass panels.
32800955	4	76	theme	FTIR	506:509	arg1	results					511:517	XRD and FTIR results	498:517	XRD and FTIR results	498:517	XRD and FTIR results showed the presence of the ZnO and chitosan.
32800955	5	77	theme	/ZnO	644:647	arg1	NRs					649:651	CHT 1%/ZnO NRs	638:651	CHT 1%/ZnO NRs	638:651	The water contact angle decreased from 80° ± 2° (control) to 65° ± 2° for CHT 1%/ZnO NRs and 42 ± 2° for CHT 2%/ZnO NRs composite coatings.
32800955	7	78	from	paint	1043:1047	arg1	experiments					1056:1066	the experiments	1052:1066	the experiments with B. subtilis and E. coli	1052:1095	CHT/ZnO composite coated fiberglass panels showed the strongest antimicrobial activity compared to chitosan, ZnO NRs coatings, and Zn-based antifouling paint in the experiments with B. subtilis and E. coli under light conditions.
32800955	5	79	theme	composite	684:692	arg1	coatings					694:701	composite coatings	684:701	composite coatings	684:701	The water contact angle decreased from 80° ± 2° (control) to 65° ± 2° for CHT 1%/ZnO NRs and 42 ± 2° for CHT 2%/ZnO NRs composite coatings.
32800955	3	80	dep	NRs	483:485	arg1	composite					487:495	composite	487:495	the CHT 2%/ZnO NRs composite	468:495	CHT 1%/ZnO composite had ZnO NRs incorporated into chitosan (CHT) coating while ZnO NRs were not visible in the CHT 2%/ZnO NRs composite.
32800955	8	81	theme	/ZnO	1177:1180	arg1	composites					1182:1191	CHT 2%/ZnO composites	1171:1191	CHT 2%/ZnO composites	1171:1191	The highest antifouling activity was observed for CHT 2%/ZnO composites.
32534086	0	0	theme	slow-release	83:94	arg1	fertilizer					100:109	slow-release DAP fertilizer	83:109	slow-release DAP fertilizer	83:109	Sustainable coating material based on chitosan-clay composite and paraffin wax for slow-release DAP fertilizer.
32534086	6	1	theme	wax	916:918	arg1	property					895:902	the hydrophobic property	879:902	the hydrophobic property of paraffin wax (outer coating)	879:934	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	6	1	theme	wax	916:918	arg1	combination					791:801	The combination	787:801	The combination of the swelling behavior of the chitosan-clay composite (inner coating)	787:873	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	3	2	from	double	418:423	arg1	paper					354:358	the present paper	342:358	the present paper	342:358	In the present paper, hydro-soluble diammonium phosphates (DAP) fertilizer was double coated following the dip-coating process by chitosan-clay composites as inner coating and paraffin wax as an outer coating.
32534086	4	3	theme	composite	562:570	arg1	preparation					572:582	The chitosan composite preparation	549:582	The chitosan composite preparation	549:582	The chitosan composite preparation and characterization were deeply investigated.
32534086	6	4	theme	paraffin	907:914	arg1	wax					916:918	paraffin wax	907:918	paraffin wax (outer coating)	907:934	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	6	4	theme	paraffin	907:914	arg1	coating					927:933	outer coating	921:933	outer coating	921:933	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	6	5	theme	behavior	819:826	arg1	property					895:902	the hydrophobic property	879:902	the hydrophobic property of paraffin wax (outer coating)	879:934	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	6	5	theme	behavior	819:826	arg1	combination					791:801	The combination	787:801	The combination of the swelling behavior of the chitosan-clay composite (inner coating)	787:873	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	3	6	theme	inner	497:501	arg1	coating					503:509	inner coating	497:509	inner coating	497:509	In the present paper, hydro-soluble diammonium phosphates (DAP) fertilizer was double coated following the dip-coating process by chitosan-clay composites as inner coating and paraffin wax as an outer coating.
32534086	4	7	theme	chitosan	553:560	arg1	preparation					572:582	The chitosan composite preparation	549:582	The chitosan composite preparation	549:582	The chitosan composite preparation and characterization were deeply investigated.
32534086	5	8	theme	mechanical	724:733	arg1	properties					735:744	mechanical properties	724:744	mechanical properties	724:744	The montmorillonite (MMT) clay incorporation as filler improves the water barrier diffusion, mechanical properties, and thermal stability of the composite.
32534086	5	9	theme	clay	657:660	arg1	incorporation					662:674	The montmorillonite (MMT) clay incorporation	631:674	The montmorillonite (MMT) clay incorporation as filler	631:684	The montmorillonite (MMT) clay incorporation as filler improves the water barrier diffusion, mechanical properties, and thermal stability of the composite.
32534086	6	10	theme	swelling	810:817	arg1	behavior					819:826	the swelling behavior	806:826	the swelling behavior of the chitosan-clay composite (inner coating)	806:873	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	8	11	theme	carbon	1342:1347	arg1	system					1301:1306	the coating system	1289:1306	the coating system proposed	1289:1315	Moreover, the biodegradation study of composite material in soil and the biochemical oxygen demand (BOD) tests revealed that the coating system proposed could be considered as a carbon source for microorganisms after the fertilization process, which confirms its sustainability.
32534086	8	11	theme	carbon	1342:1347	arg1	source					1349:1354	a carbon source	1340:1354	a carbon source	1340:1354	Moreover, the biodegradation study of composite material in soil and the biochemical oxygen demand (BOD) tests revealed that the coating system proposed could be considered as a carbon source for microorganisms after the fertilization process, which confirms its sustainability.
32534086	6	12	theme	capacity	971:978	arg1	evaluation					980:989	the water holding capacity evaluation	953:989	the water holding capacity evaluation	953:989	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	0	13	theme	DAP	96:98	arg1	fertilizer					100:109	slow-release DAP fertilizer	83:109	slow-release DAP fertilizer	83:109	Sustainable coating material based on chitosan-clay composite and paraffin wax for slow-release DAP fertilizer.
32534086	7	14	theme	DAP	1095:1097	arg1	granules					1099:1106	the coated DAP granules	1084:1106	the coated DAP granules	1084:1106	Indeed, the phosphorus dissolution from the coated DAP granules was significantly delayed compared to the uncoated DAP.
32534086	6	15	theme	holding	963:969	arg1	evaluation					980:989	the water holding capacity evaluation	953:989	the water holding capacity evaluation	953:989	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	3	16	from	paper	354:358	arg1	double					418:423	double	418:423	double	418:423	In the present paper, hydro-soluble diammonium phosphates (DAP) fertilizer was double coated following the dip-coating process by chitosan-clay composites as inner coating and paraffin wax as an outer coating.
32534086	8	17	theme	biochemical	1237:1247	arg1	BOD					1264:1266	BOD	1264:1266	BOD	1264:1266	Moreover, the biodegradation study of composite material in soil and the biochemical oxygen demand (BOD) tests revealed that the coating system proposed could be considered as a carbon source for microorganisms after the fertilization process, which confirms its sustainability.
32534086	8	17	theme	biochemical	1237:1247	arg1	demand					1256:1261	the biochemical oxygen demand	1233:1261	the biochemical oxygen demand (BOD)	1233:1267	Moreover, the biodegradation study of composite material in soil and the biochemical oxygen demand (BOD) tests revealed that the coating system proposed could be considered as a carbon source for microorganisms after the fertilization process, which confirms its sustainability.
32534086	3	18	theme	paraffin	515:522	arg1	wax					524:526	paraffin wax	515:526	paraffin wax	515:526	In the present paper, hydro-soluble diammonium phosphates (DAP) fertilizer was double coated following the dip-coating process by chitosan-clay composites as inner coating and paraffin wax as an outer coating.
32534086	0	19	theme	composite	52:60	arg1	wax					75:77	chitosan-clay composite and paraffin wax	38:77	chitosan-clay composite and paraffin wax for slow-release DAP fertilizer	38:109	Sustainable coating material based on chitosan-clay composite and paraffin wax for slow-release DAP fertilizer.
32534086	5	20	theme	composite	776:784	arg1	stability					759:767	thermal stability	751:767	thermal stability	751:767	The montmorillonite (MMT) clay incorporation as filler improves the water barrier diffusion, mechanical properties, and thermal stability of the composite.
32534086	5	20	theme	composite	776:784	arg1	diffusion					713:721	the water barrier diffusion	695:721	the water barrier diffusion	695:721	The montmorillonite (MMT) clay incorporation as filler improves the water barrier diffusion, mechanical properties, and thermal stability of the composite.
32534086	5	20	theme	composite	776:784	arg1	properties					735:744	mechanical properties	724:744	mechanical properties	724:744	The montmorillonite (MMT) clay incorporation as filler improves the water barrier diffusion, mechanical properties, and thermal stability of the composite.
32534086	2	21	theme	excessive	253:261	arg1	use					263:265	excessive use	253:265	excessive use of the fertilizers	253:284	This strategy avoids excessive use of the fertilizers and increases their availability to the crops needs.
32534086	8	22	from	study	1193:1197	arg1	BOD					1264:1266	BOD	1264:1266	BOD	1264:1266	Moreover, the biodegradation study of composite material in soil and the biochemical oxygen demand (BOD) tests revealed that the coating system proposed could be considered as a carbon source for microorganisms after the fertilization process, which confirms its sustainability.
32534086	8	22	from	study	1193:1197	arg1	demand					1256:1261	the biochemical oxygen demand	1233:1261	the biochemical oxygen demand (BOD)	1233:1267	Moreover, the biodegradation study of composite material in soil and the biochemical oxygen demand (BOD) tests revealed that the coating system proposed could be considered as a carbon source for microorganisms after the fertilization process, which confirms its sustainability.
32534086	8	22	from	study	1193:1197	arg1	soil					1224:1227	soil	1224:1227	soil	1224:1227	Moreover, the biodegradation study of composite material in soil and the biochemical oxygen demand (BOD) tests revealed that the coating system proposed could be considered as a carbon source for microorganisms after the fertilization process, which confirms its sustainability.
32534086	7	23	theme	phosphorus	1056:1065	arg1	dissolution					1067:1077	the phosphorus dissolution	1052:1077	the phosphorus dissolution from the coated DAP granules	1052:1106	Indeed, the phosphorus dissolution from the coated DAP granules was significantly delayed compared to the uncoated DAP.
32534086	6	24	theme	hydrophobic	883:893	arg1	property					895:902	the hydrophobic property	879:902	the hydrophobic property of paraffin wax (outer coating)	879:934	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	1	25	theme	efficient	170:178	arg1	tools					180:184	the most efficient tools	161:184	the most efficient tools for their slow and control release into soil	161:229	The coating of fertilizers by polymers is one of the most efficient tools for their slow and control release into soil.
32534086	3	26	theme	hydro-soluble	361:373	arg1	phosphates					386:395	hydro-soluble diammonium phosphates	361:395	hydro-soluble diammonium phosphates (DAP) fertilizer	361:412	In the present paper, hydro-soluble diammonium phosphates (DAP) fertilizer was double coated following the dip-coating process by chitosan-clay composites as inner coating and paraffin wax as an outer coating.
32534086	3	26	theme	hydro-soluble	361:373	arg1	DAP					398:400	DAP	398:400	DAP	398:400	In the present paper, hydro-soluble diammonium phosphates (DAP) fertilizer was double coated following the dip-coating process by chitosan-clay composites as inner coating and paraffin wax as an outer coating.
32534086	6	27	theme	water	957:961	arg1	evaluation					980:989	the water holding capacity evaluation	953:989	the water holding capacity evaluation	953:989	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	6	28	from	evaluation	980:989	arg1	soil					1038:1041	soil	1038:1041	soil	1038:1041	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	6	28	from	evaluation	980:989	arg1	water					1028:1032	water	1028:1032	water	1028:1032	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	1	29	theme	tools	180:184	arg1	coating					116:122	The coating	112:122	The coating of fertilizers by polymers	112:149	The coating of fertilizers by polymers is one of the most efficient tools for their slow and control release into soil.
32534086	1	29	theme	tools	180:184	arg1	tools					180:184	the most efficient tools	161:184	the most efficient tools for their slow and control release into soil	161:229	The coating of fertilizers by polymers is one of the most efficient tools for their slow and control release into soil.
32534086	1	29	theme	tools	180:184	arg1	one					154:156	one	154:156	one	154:156	The coating of fertilizers by polymers is one of the most efficient tools for their slow and control release into soil.
32534086	3	30	theme	present	346:352	arg1	paper					354:358	the present paper	342:358	the present paper	342:358	In the present paper, hydro-soluble diammonium phosphates (DAP) fertilizer was double coated following the dip-coating process by chitosan-clay composites as inner coating and paraffin wax as an outer coating.
32534086	5	31	theme	water	699:703	arg1	diffusion					713:721	the water barrier diffusion	695:721	the water barrier diffusion	695:721	The montmorillonite (MMT) clay incorporation as filler improves the water barrier diffusion, mechanical properties, and thermal stability of the composite.
32534086	3	32	theme	outer	534:538	arg1	coating					540:546	an outer coating	531:546	an outer coating	531:546	In the present paper, hydro-soluble diammonium phosphates (DAP) fertilizer was double coated following the dip-coating process by chitosan-clay composites as inner coating and paraffin wax as an outer coating.
32534086	8	33	theme	oxygen	1249:1254	arg1	BOD					1264:1266	BOD	1264:1266	BOD	1264:1266	Moreover, the biodegradation study of composite material in soil and the biochemical oxygen demand (BOD) tests revealed that the coating system proposed could be considered as a carbon source for microorganisms after the fertilization process, which confirms its sustainability.
32534086	8	33	theme	oxygen	1249:1254	arg1	demand					1256:1261	the biochemical oxygen demand	1233:1261	the biochemical oxygen demand (BOD)	1233:1267	Moreover, the biodegradation study of composite material in soil and the biochemical oxygen demand (BOD) tests revealed that the coating system proposed could be considered as a carbon source for microorganisms after the fertilization process, which confirms its sustainability.
32534086	7	34	theme	uncoated	1150:1157	arg1	DAP					1159:1161	the uncoated DAP	1146:1161	the uncoated DAP	1146:1161	Indeed, the phosphorus dissolution from the coated DAP granules was significantly delayed compared to the uncoated DAP.
32534086	5	35	theme	barrier	705:711	arg1	diffusion					713:721	the water barrier diffusion	695:721	the water barrier diffusion	695:721	The montmorillonite (MMT) clay incorporation as filler improves the water barrier diffusion, mechanical properties, and thermal stability of the composite.
32534086	2	36	theme	fertilizers	274:284	arg1	use					263:265	excessive use	253:265	excessive use of the fertilizers	253:284	This strategy avoids excessive use of the fertilizers and increases their availability to the crops needs.
32534086	3	37	theme	dip-coating	446:456	arg1	process					458:464	the dip-coating process	442:464	the dip-coating process by chitosan-clay composites as inner coating and paraffin wax as an outer coating	442:546	In the present paper, hydro-soluble diammonium phosphates (DAP) fertilizer was double coated following the dip-coating process by chitosan-clay composites as inner coating and paraffin wax as an outer coating.
32534086	0	38	theme	chitosan-clay	38:50	arg1	wax					75:77	chitosan-clay composite and paraffin wax	38:77	chitosan-clay composite and paraffin wax for slow-release DAP fertilizer	38:109	Sustainable coating material based on chitosan-clay composite and paraffin wax for slow-release DAP fertilizer.
32534086	8	39	theme	coating	1293:1299	arg1	source					1349:1354	a carbon source	1340:1354	a carbon source	1340:1354	Moreover, the biodegradation study of composite material in soil and the biochemical oxygen demand (BOD) tests revealed that the coating system proposed could be considered as a carbon source for microorganisms after the fertilization process, which confirms its sustainability.
32534086	8	39	theme	coating	1293:1299	arg1	system					1301:1306	the coating system	1289:1306	the coating system proposed	1289:1315	Moreover, the biodegradation study of composite material in soil and the biochemical oxygen demand (BOD) tests revealed that the coating system proposed could be considered as a carbon source for microorganisms after the fertilization process, which confirms its sustainability.
32534086	1	40	theme	slow	196:199	arg1	release					213:219	their slow and control release	190:219	their slow and control release into soil	190:229	The coating of fertilizers by polymers is one of the most efficient tools for their slow and control release into soil.
32534086	6	41	theme	inner	860:864	arg1	composite					849:857	the chitosan-clay composite	831:857	the chitosan-clay composite (inner coating)	831:873	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	6	41	theme	inner	860:864	arg1	coating					866:872	inner coating	860:872	inner coating	860:872	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	8	42	theme	biodegradation	1178:1191	arg1	study					1193:1197	the biodegradation study	1174:1197	the biodegradation study of composite material in soil and the biochemical oxygen demand (BOD) tests	1174:1273	Moreover, the biodegradation study of composite material in soil and the biochemical oxygen demand (BOD) tests revealed that the coating system proposed could be considered as a carbon source for microorganisms after the fertilization process, which confirms its sustainability.
32534086	3	43	theme	diammonium	375:384	arg1	phosphates					386:395	hydro-soluble diammonium phosphates	361:395	hydro-soluble diammonium phosphates (DAP) fertilizer	361:412	In the present paper, hydro-soluble diammonium phosphates (DAP) fertilizer was double coated following the dip-coating process by chitosan-clay composites as inner coating and paraffin wax as an outer coating.
32534086	3	43	theme	diammonium	375:384	arg1	DAP					398:400	DAP	398:400	DAP	398:400	In the present paper, hydro-soluble diammonium phosphates (DAP) fertilizer was double coated following the dip-coating process by chitosan-clay composites as inner coating and paraffin wax as an outer coating.
32534086	3	44	theme	chitosan-clay	469:481	arg1	composites					483:492	chitosan-clay composites	469:492	chitosan-clay composites as inner coating and paraffin wax	469:526	In the present paper, hydro-soluble diammonium phosphates (DAP) fertilizer was double coated following the dip-coating process by chitosan-clay composites as inner coating and paraffin wax as an outer coating.
32534086	3	45	theme	phosphates	386:395	arg1	fertilizer					403:412	hydro-soluble diammonium phosphates (DAP) fertilizer	361:412	hydro-soluble diammonium phosphates (DAP) fertilizer	361:412	In the present paper, hydro-soluble diammonium phosphates (DAP) fertilizer was double coated following the dip-coating process by chitosan-clay composites as inner coating and paraffin wax as an outer coating.
32534086	7	46	from	granules	1099:1106	arg1	dissolution					1067:1077	the phosphorus dissolution	1052:1077	the phosphorus dissolution from the coated DAP granules	1052:1106	Indeed, the phosphorus dissolution from the coated DAP granules was significantly delayed compared to the uncoated DAP.
32534086	6	47	theme	composite	849:857	arg1	behavior					819:826	the swelling behavior	806:826	the swelling behavior of the chitosan-clay composite (inner coating)	806:873	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	6	48	from	essays	1018:1023	arg1	soil					1038:1041	soil	1038:1041	soil	1038:1041	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	6	48	from	essays	1018:1023	arg1	water					1028:1032	water	1028:1032	water	1028:1032	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	6	49	theme	outer	921:925	arg1	wax					916:918	paraffin wax	907:918	paraffin wax (outer coating)	907:934	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	6	49	theme	outer	921:925	arg1	coating					927:933	outer coating	921:933	outer coating	921:933	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	6	50	theme	chitosan-clay	835:847	arg1	composite					849:857	the chitosan-clay composite	831:857	the chitosan-clay composite (inner coating)	831:873	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	6	50	theme	chitosan-clay	835:847	arg1	coating					866:872	inner coating	860:872	inner coating	860:872	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	1	51	theme	fertilizers	127:137	arg1	coating					116:122	The coating	112:122	The coating of fertilizers by polymers	112:149	The coating of fertilizers by polymers is one of the most efficient tools for their slow and control release into soil.
32534086	1	51	theme	fertilizers	127:137	arg1	tools					180:184	the most efficient tools	161:184	the most efficient tools for their slow and control release into soil	161:229	The coating of fertilizers by polymers is one of the most efficient tools for their slow and control release into soil.
32534086	1	51	theme	fertilizers	127:137	arg1	one					154:156	one	154:156	one	154:156	The coating of fertilizers by polymers is one of the most efficient tools for their slow and control release into soil.
32534086	2	52	dep	crops	326:330	arg1	needs					332:336	needs	332:336	needs	332:336	This strategy avoids excessive use of the fertilizers and increases their availability to the crops needs.
32534086	1	53	theme	control	205:211	arg1	release					213:219	their slow and control release	190:219	their slow and control release into soil	190:229	The coating of fertilizers by polymers is one of the most efficient tools for their slow and control release into soil.
32534086	8	54	theme	composite	1202:1210	arg1	material					1212:1219	composite material	1202:1219	composite material	1202:1219	Moreover, the biodegradation study of composite material in soil and the biochemical oxygen demand (BOD) tests revealed that the coating system proposed could be considered as a carbon source for microorganisms after the fertilization process, which confirms its sustainability.
32534086	8	55	theme	fertilization	1385:1397	arg1	process					1399:1405	the fertilization process	1381:1405	the fertilization process	1381:1405	Moreover, the biodegradation study of composite material in soil and the biochemical oxygen demand (BOD) tests revealed that the coating system proposed could be considered as a carbon source for microorganisms after the fertilization process, which confirms its sustainability.
32534086	7	56	theme	coated	1088:1093	arg1	granules					1099:1106	the coated DAP granules	1084:1106	the coated DAP granules	1084:1106	Indeed, the phosphorus dissolution from the coated DAP granules was significantly delayed compared to the uncoated DAP.
32534086	8	57	theme	material	1212:1219	arg1	study					1193:1197	the biodegradation study	1174:1197	the biodegradation study of composite material in soil and the biochemical oxygen demand (BOD) tests	1174:1273	Moreover, the biodegradation study of composite material in soil and the biochemical oxygen demand (BOD) tests revealed that the coating system proposed could be considered as a carbon source for microorganisms after the fertilization process, which confirms its sustainability.
32534086	6	58	theme	release	1010:1016	arg1	essays					1018:1023	the phosphorus release essays	995:1023	the phosphorus release essays in water and soil	995:1041	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	5	59	theme	thermal	751:757	arg1	stability					759:767	thermal stability	751:767	thermal stability	751:767	The montmorillonite (MMT) clay incorporation as filler improves the water barrier diffusion, mechanical properties, and thermal stability of the composite.
32534086	8	60	dep	soil	1224:1227	arg1	tests					1269:1273	tests	1269:1273	tests	1269:1273	Moreover, the biodegradation study of composite material in soil and the biochemical oxygen demand (BOD) tests revealed that the coating system proposed could be considered as a carbon source for microorganisms after the fertilization process, which confirms its sustainability.
32534086	6	61	theme	phosphorus	999:1008	arg1	essays					1018:1023	the phosphorus release essays	995:1023	the phosphorus release essays in water and soil	995:1041	The combination of the swelling behavior of the chitosan-clay composite (inner coating) and the hydrophobic property of paraffin wax (outer coating) was confirmed by the water holding capacity evaluation and the phosphorus release essays in water and soil.
32534086	0	62	theme	paraffin	66:73	arg1	wax					75:77	chitosan-clay composite and paraffin wax	38:77	chitosan-clay composite and paraffin wax for slow-release DAP fertilizer	38:109	Sustainable coating material based on chitosan-clay composite and paraffin wax for slow-release DAP fertilizer.
33142567	0	0	theme	Recyclable	81:90	arg1	catalysis					92:100	Recyclable catalysis	81:100	Recyclable catalysis	81:100	In situ synthesis of gold nanoparticles on novel nanocomposite lactose/alginate: Recyclable catalysis and colorimetric detection of Fe(III).
33142567	0	1	from	synthesis	8:16	arg1	lactose/alginate					63:78	novel nanocomposite lactose/alginate	43:78	novel nanocomposite lactose/alginate	43:78	In situ synthesis of gold nanoparticles on novel nanocomposite lactose/alginate: Recyclable catalysis and colorimetric detection of Fe(III).
33142567	5	2	theme	methyl	748:753	arg1	orange					755:760	methyl orange	748:760	methyl orange	748:760	The nanocomposite powder possesses highly catalytic performance for degradation of contaminants including 4-nitrophenol, methyl orange, rhodamine 6 G and rhodamine B.
33142567	3	3	theme	reducing	465:472	arg1	role					455:458	a crucial role	445:458	a crucial role	445:458	Lactose plays a crucial role as a reducing reagent which are demonstrated by FTIR analysis.
33142567	3	3	theme	reducing	465:472	arg1	reagent					474:480	a reducing reagent	463:480	a reducing reagent which are demonstrated by FTIR analysis	463:520	Lactose plays a crucial role as a reducing reagent which are demonstrated by FTIR analysis.
33142567	6	4	theme	effective	850:858	arg1	probe					860:864	an effective probe	847:864	an effective probe for highly selective detection of Fe3+ ions	847:908	The dispersion solution of AuNPs@Lac/Alg was used as an effective probe for highly selective detection of Fe3+ ions.
33142567	6	4	theme	effective	850:858	arg1	solution					809:816	The dispersion solution	794:816	The dispersion solution of AuNPs@Lac/Alg	794:833	The dispersion solution of AuNPs@Lac/Alg was used as an effective probe for highly selective detection of Fe3+ ions.
33142567	7	5	theme	detection	915:923	arg1	replies					935:941	The detection mechanism replies	911:941	The detection mechanism replies on the aggregation of	911:963	The detection mechanism replies on the aggregation of nanocomposite in the presence of Fe3+ ions.
33142567	5	6	theme	rhodamine	763:771	arg1	G					775:775	rhodamine 6 G	763:775	rhodamine 6 G	763:775	The nanocomposite powder possesses highly catalytic performance for degradation of contaminants including 4-nitrophenol, methyl orange, rhodamine 6 G and rhodamine B.
33142567	8	7	from	μM	1039:1040	arg1	range					1054:1058	a linear range	1045:1058	a linear range of 2.0-80.0 μM	1045:1073	LOD value was found to be 0.8 μM in a linear range of 2.0-80.0 μM.
33142567	8	8	theme	μM	1072:1073	arg1	range					1054:1058	a linear range	1045:1058	a linear range of 2.0-80.0 μM	1045:1073	LOD value was found to be 0.8 μM in a linear range of 2.0-80.0 μM.
33142567	3	9	theme	FTIR	508:511	arg1	analysis					513:520	FTIR analysis	508:520	FTIR analysis	508:520	Lactose plays a crucial role as a reducing reagent which are demonstrated by FTIR analysis.
33142567	3	10	theme	crucial	447:453	arg1	role					455:458	a crucial role	445:458	a crucial role	445:458	Lactose plays a crucial role as a reducing reagent which are demonstrated by FTIR analysis.
33142567	3	10	theme	crucial	447:453	arg1	reagent					474:480	a reducing reagent	463:480	a reducing reagent which are demonstrated by FTIR analysis	463:520	Lactose plays a crucial role as a reducing reagent which are demonstrated by FTIR analysis.
33142567	4	11	theme	AuNPs	552:556	arg1	structure					539:547	The crystalline structure	523:547	The crystalline structure of AuNPs with a mean size of 10 nm	523:582	The crystalline structure of AuNPs with a mean size of 10 nm has been confirmed by analysis techniques.
33142567	0	12	theme	colorimetric	106:117	arg1	detection					119:127	colorimetric detection	106:127	colorimetric detection	106:127	In situ synthesis of gold nanoparticles on novel nanocomposite lactose/alginate: Recyclable catalysis and colorimetric detection of Fe(III).
33142567	0	13	from	nanoparticles	26:38	arg1	lactose/alginate					63:78	novel nanocomposite lactose/alginate	43:78	novel nanocomposite lactose/alginate	43:78	In situ synthesis of gold nanoparticles on novel nanocomposite lactose/alginate: Recyclable catalysis and colorimetric detection of Fe(III).
33142567	2	14	theme	gelation	365:372	arg1	mechanism					374:382	ionotropic gelation mechanism	354:382	ionotropic gelation mechanism which can reduce in situ gold ions into AuNPs	354:428	A novel composite of lactose/alginate (Lac/Alg) could be prepared easily through ionotropic gelation mechanism which can reduce in situ gold ions into AuNPs.
33142567	6	15	theme	ions	905:908	arg1	detection					887:895	highly selective detection	870:895	highly selective detection of Fe3+ ions	870:908	The dispersion solution of AuNPs@Lac/Alg was used as an effective probe for highly selective detection of Fe3+ ions.
33142567	7	16	theme	ions	1003:1006	arg1	presence					986:993	the presence	982:993	the presence of Fe3+ ions	982:1006	The detection mechanism replies on the aggregation of nanocomposite in the presence of Fe3+ ions.
33142567	2	17	theme	ionotropic	354:363	arg1	mechanism					374:382	ionotropic gelation mechanism	354:382	ionotropic gelation mechanism which can reduce in situ gold ions into AuNPs	354:428	A novel composite of lactose/alginate (Lac/Alg) could be prepared easily through ionotropic gelation mechanism which can reduce in situ gold ions into AuNPs.
33142567	0	18	theme	In	0:1	arg1	synthesis					8:16	In situ synthesis	0:16	In situ synthesis of gold nanoparticles on novel nanocomposite lactose/alginate: Recyclable catalysis and colorimetric detection of Fe(III).	0:139	In situ synthesis of gold nanoparticles on novel nanocomposite lactose/alginate: Recyclable catalysis and colorimetric detection of Fe(III).
33142567	6	19	theme	selective	877:885	arg1	detection					887:895	highly selective detection	870:895	highly selective detection of Fe3+ ions	870:908	The dispersion solution of AuNPs@Lac/Alg was used as an effective probe for highly selective detection of Fe3+ ions.
33142567	1	20	theme	new	169:171	arg1	method					173:178	a simply new method	160:178	a simply new method for in situ synthesis of gold nanoparticles (AuNPs) using the biodegradable polysaccharides	160:270	This work presents a simply new method for in situ synthesis of gold nanoparticles (AuNPs) using the biodegradable polysaccharides.
33142567	2	21	theme	gold	409:412	arg1	ions					414:417	in situ gold ions	401:417	in situ gold ions	401:417	A novel composite of lactose/alginate (Lac/Alg) could be prepared easily through ionotropic gelation mechanism which can reduce in situ gold ions into AuNPs.
33142567	1	22	theme	biodegradable	242:254	arg1	polysaccharides					256:270	the biodegradable polysaccharides	238:270	the biodegradable polysaccharides	238:270	This work presents a simply new method for in situ synthesis of gold nanoparticles (AuNPs) using the biodegradable polysaccharides.
33142567	0	23	theme	Fe	132:133	arg1	catalysis					92:100	Recyclable catalysis	81:100	Recyclable catalysis	81:100	In situ synthesis of gold nanoparticles on novel nanocomposite lactose/alginate: Recyclable catalysis and colorimetric detection of Fe(III).
33142567	0	23	theme	Fe	132:133	arg1	detection					119:127	colorimetric detection	106:127	colorimetric detection	106:127	In situ synthesis of gold nanoparticles on novel nanocomposite lactose/alginate: Recyclable catalysis and colorimetric detection of Fe(III).
33142567	2	24	theme	novel	275:279	arg1	lactose/alginate					294:309	lactose/alginate	294:309	lactose/alginate (Lac/Alg)	294:319	A novel composite of lactose/alginate (Lac/Alg) could be prepared easily through ionotropic gelation mechanism which can reduce in situ gold ions into AuNPs.
33142567	2	24	theme	novel	275:279	arg1	composite					281:289	A novel composite	273:289	A novel composite of lactose/alginate (Lac/Alg)	273:319	A novel composite of lactose/alginate (Lac/Alg) could be prepared easily through ionotropic gelation mechanism which can reduce in situ gold ions into AuNPs.
33142567	2	25	theme	in	401:402	arg1	ions					414:417	in situ gold ions	401:417	in situ gold ions	401:417	A novel composite of lactose/alginate (Lac/Alg) could be prepared easily through ionotropic gelation mechanism which can reduce in situ gold ions into AuNPs.
33142567	4	26	theme	crystalline	527:537	arg1	structure					539:547	The crystalline structure	523:547	The crystalline structure of AuNPs with a mean size of 10 nm	523:582	The crystalline structure of AuNPs with a mean size of 10 nm has been confirmed by analysis techniques.
33142567	6	27	theme	Lac/Alg	827:833	arg1	probe					860:864	an effective probe	847:864	an effective probe for highly selective detection of Fe3+ ions	847:908	The dispersion solution of AuNPs@Lac/Alg was used as an effective probe for highly selective detection of Fe3+ ions.
33142567	6	27	theme	Lac/Alg	827:833	arg1	solution					809:816	The dispersion solution	794:816	The dispersion solution of AuNPs@Lac/Alg	794:833	The dispersion solution of AuNPs@Lac/Alg was used as an effective probe for highly selective detection of Fe3+ ions.
33142567	0	28	theme	nanoparticles	26:38	arg1	synthesis					8:16	In situ synthesis	0:16	In situ synthesis of gold nanoparticles on novel nanocomposite lactose/alginate: Recyclable catalysis and colorimetric detection of Fe(III).	0:139	In situ synthesis of gold nanoparticles on novel nanocomposite lactose/alginate: Recyclable catalysis and colorimetric detection of Fe(III).
33142567	5	29	contain	possesses	652:660	arg2	performance					679:689	highly catalytic performance	662:689	highly catalytic performance for degradation of contaminants including 4-nitrophenol, methyl orange, rhodamine 6 G and rhodamine B	662:791	The nanocomposite powder possesses highly catalytic performance for degradation of contaminants including 4-nitrophenol, methyl orange, rhodamine 6 G and rhodamine B.
33142567	5	29	contain	possesses	652:660	arg1	powder					645:650	The nanocomposite powder	627:650	The nanocomposite powder	627:650	The nanocomposite powder possesses highly catalytic performance for degradation of contaminants including 4-nitrophenol, methyl orange, rhodamine 6 G and rhodamine B.
33142567	5	30	theme	catalytic	669:677	arg1	performance					679:689	highly catalytic performance	662:689	highly catalytic performance for degradation of contaminants including 4-nitrophenol, methyl orange, rhodamine 6 G and rhodamine B	662:791	The nanocomposite powder possesses highly catalytic performance for degradation of contaminants including 4-nitrophenol, methyl orange, rhodamine 6 G and rhodamine B.
33142567	6	31	theme	@	826:826	arg1	Lac/Alg					827:833	AuNPs@Lac/Alg	821:833	AuNPs@Lac/Alg	821:833	The dispersion solution of AuNPs@Lac/Alg was used as an effective probe for highly selective detection of Fe3+ ions.
33142567	4	32	theme	analysis	606:613	arg1	techniques					615:624	analysis techniques	606:624	analysis techniques	606:624	The crystalline structure of AuNPs with a mean size of 10 nm has been confirmed by analysis techniques.
33142567	1	33	theme	in	184:185	arg1	synthesis					192:200	in situ synthesis	184:200	in situ synthesis of gold nanoparticles (AuNPs) using the biodegradable polysaccharides	184:270	This work presents a simply new method for in situ synthesis of gold nanoparticles (AuNPs) using the biodegradable polysaccharides.
33142567	0	34	theme	gold	21:24	arg1	nanoparticles					26:38	gold nanoparticles	21:38	gold nanoparticles on novel nanocomposite lactose/alginate	21:78	In situ synthesis of gold nanoparticles on novel nanocomposite lactose/alginate: Recyclable catalysis and colorimetric detection of Fe(III).
33142567	6	35	theme	Fe3+	900:903	arg1	ions					905:908	Fe3+ ions	900:908	Fe3+ ions	900:908	The dispersion solution of AuNPs@Lac/Alg was used as an effective probe for highly selective detection of Fe3+ ions.
33142567	0	36	from	lactose/alginate	63:78	arg1	synthesis					8:16	In situ synthesis	0:16	In situ synthesis of gold nanoparticles on novel nanocomposite lactose/alginate: Recyclable catalysis and colorimetric detection of Fe(III).	0:139	In situ synthesis of gold nanoparticles on novel nanocomposite lactose/alginate: Recyclable catalysis and colorimetric detection of Fe(III).
33142567	5	37	theme	rhodamine	781:789	arg1	B					791:791	rhodamine B	781:791	rhodamine B	781:791	The nanocomposite powder possesses highly catalytic performance for degradation of contaminants including 4-nitrophenol, methyl orange, rhodamine 6 G and rhodamine B.
33142567	5	38	theme	contaminants	710:721	arg1	degradation					695:705	degradation	695:705	degradation of contaminants including 4-nitrophenol, methyl orange, rhodamine 6 G and rhodamine B	695:791	The nanocomposite powder possesses highly catalytic performance for degradation of contaminants including 4-nitrophenol, methyl orange, rhodamine 6 G and rhodamine B.
33142567	0	39	theme	novel	43:47	arg1	lactose/alginate					63:78	novel nanocomposite lactose/alginate	43:78	novel nanocomposite lactose/alginate	43:78	In situ synthesis of gold nanoparticles on novel nanocomposite lactose/alginate: Recyclable catalysis and colorimetric detection of Fe(III).
33142567	6	40	used	used	839:842	arg2	probe					860:864	an effective probe	847:864	an effective probe for highly selective detection of Fe3+ ions	847:908	The dispersion solution of AuNPs@Lac/Alg was used as an effective probe for highly selective detection of Fe3+ ions.
33142567	6	40	used	used	839:842	arg2	solution					809:816	The dispersion solution	794:816	The dispersion solution of AuNPs@Lac/Alg	794:833	The dispersion solution of AuNPs@Lac/Alg was used as an effective probe for highly selective detection of Fe3+ ions.
33142567	2	41	dep	in	401:402	arg1	situ					404:407	situ	404:407	situ	404:407	A novel composite of lactose/alginate (Lac/Alg) could be prepared easily through ionotropic gelation mechanism which can reduce in situ gold ions into AuNPs.
33142567	7	42	theme	Fe3+	998:1001	arg1	ions					1003:1006	Fe3+ ions	998:1006	Fe3+ ions	998:1006	The detection mechanism replies on the aggregation of nanocomposite in the presence of Fe3+ ions.
33142567	6	43	theme	AuNPs	821:825	arg1	Lac/Alg					827:833	AuNPs@Lac/Alg	821:833	AuNPs@Lac/Alg	821:833	The dispersion solution of AuNPs@Lac/Alg was used as an effective probe for highly selective detection of Fe3+ ions.
33142567	4	44	with	structure	539:547	arg1	size					570:573	a mean size	563:573	a mean size of 10 nm	563:582	The crystalline structure of AuNPs with a mean size of 10 nm has been confirmed by analysis techniques.
33142567	5	45	theme	nanocomposite	631:643	arg1	powder					645:650	The nanocomposite powder	627:650	The nanocomposite powder	627:650	The nanocomposite powder possesses highly catalytic performance for degradation of contaminants including 4-nitrophenol, methyl orange, rhodamine 6 G and rhodamine B.
33142567	6	46	theme	dispersion	798:807	arg1	probe					860:864	an effective probe	847:864	an effective probe for highly selective detection of Fe3+ ions	847:908	The dispersion solution of AuNPs@Lac/Alg was used as an effective probe for highly selective detection of Fe3+ ions.
33142567	6	46	theme	dispersion	798:807	arg1	solution					809:816	The dispersion solution	794:816	The dispersion solution of AuNPs@Lac/Alg	794:833	The dispersion solution of AuNPs@Lac/Alg was used as an effective probe for highly selective detection of Fe3+ ions.
33142567	1	47	dep	in	184:185	arg1	situ					187:190	situ	187:190	situ	187:190	This work presents a simply new method for in situ synthesis of gold nanoparticles (AuNPs) using the biodegradable polysaccharides.
33142567	4	48	theme	mean	565:568	arg1	size					570:573	a mean size	563:573	a mean size of 10 nm	563:582	The crystalline structure of AuNPs with a mean size of 10 nm has been confirmed by analysis techniques.
33142567	0	49	dep	synthesis	8:16	arg1	catalysis					92:100	Recyclable catalysis	81:100	Recyclable catalysis	81:100	In situ synthesis of gold nanoparticles on novel nanocomposite lactose/alginate: Recyclable catalysis and colorimetric detection of Fe(III).
33142567	0	49	dep	synthesis	8:16	arg1	detection					119:127	colorimetric detection	106:127	colorimetric detection	106:127	In situ synthesis of gold nanoparticles on novel nanocomposite lactose/alginate: Recyclable catalysis and colorimetric detection of Fe(III).
33142567	2	50	theme	lactose/alginate	294:309	arg1	lactose/alginate					294:309	lactose/alginate	294:309	lactose/alginate (Lac/Alg)	294:319	A novel composite of lactose/alginate (Lac/Alg) could be prepared easily through ionotropic gelation mechanism which can reduce in situ gold ions into AuNPs.
33142567	2	50	theme	lactose/alginate	294:309	arg1	composite					281:289	A novel composite	273:289	A novel composite of lactose/alginate (Lac/Alg)	273:319	A novel composite of lactose/alginate (Lac/Alg) could be prepared easily through ionotropic gelation mechanism which can reduce in situ gold ions into AuNPs.
33142567	8	51	theme	linear	1047:1052	arg1	range					1054:1058	a linear range	1045:1058	a linear range of 2.0-80.0 μM	1045:1073	LOD value was found to be 0.8 μM in a linear range of 2.0-80.0 μM.
33142567	8	52	theme	LOD	1009:1011	arg1	value					1013:1017	LOD value	1009:1017	LOD value	1009:1017	LOD value was found to be 0.8 μM in a linear range of 2.0-80.0 μM.
33142567	4	53	theme	nm	581:582	arg1	size					570:573	a mean size	563:573	a mean size of 10 nm	563:582	The crystalline structure of AuNPs with a mean size of 10 nm has been confirmed by analysis techniques.
33142567	7	54	from	replies	935:941	arg1	aggregation					950:960	the aggregation	946:960	the aggregation of	946:963	The detection mechanism replies on the aggregation of nanocomposite in the presence of Fe3+ ions.
33142567	1	55	theme	gold	205:208	arg1	AuNPs					225:229	AuNPs	225:229	AuNPs	225:229	This work presents a simply new method for in situ synthesis of gold nanoparticles (AuNPs) using the biodegradable polysaccharides.
33142567	1	55	theme	gold	205:208	arg1	nanoparticles					210:222	gold nanoparticles	205:222	gold nanoparticles (AuNPs) using the biodegradable polysaccharides	205:270	This work presents a simply new method for in situ synthesis of gold nanoparticles (AuNPs) using the biodegradable polysaccharides.
33142567	0	56	theme	nanocomposite	49:61	arg1	lactose/alginate					63:78	novel nanocomposite lactose/alginate	43:78	novel nanocomposite lactose/alginate	43:78	In situ synthesis of gold nanoparticles on novel nanocomposite lactose/alginate: Recyclable catalysis and colorimetric detection of Fe(III).
33142567	0	57	dep	In	0:1	arg1	situ					3:6	situ	3:6	situ	3:6	In situ synthesis of gold nanoparticles on novel nanocomposite lactose/alginate: Recyclable catalysis and colorimetric detection of Fe(III).
33142567	7	58	theme	mechanism	925:933	arg1	replies					935:941	The detection mechanism replies	911:941	The detection mechanism replies on the aggregation of	911:963	The detection mechanism replies on the aggregation of nanocomposite in the presence of Fe3+ ions.
33142567	1	59	theme	nanoparticles	210:222	arg1	synthesis					192:200	in situ synthesis	184:200	in situ synthesis of gold nanoparticles (AuNPs) using the biodegradable polysaccharides	184:270	This work presents a simply new method for in situ synthesis of gold nanoparticles (AuNPs) using the biodegradable polysaccharides.
33358974	7	0	theme	polymer	1199:1205	arg1	wafers					1207:1212	the CMC/ALG binary blend polymer wafers	1174:1212	the CMC/ALG binary blend polymer wafers	1174:1212	These findings established that the CMC/ALG binary blend polymer wafers have the potential to improve the sublingual delivery and storage stability of protein-based vaccines.
33358974	7	1	contain	have	1214:1217	arg1	wafers					1207:1212	the CMC/ALG binary blend polymer wafers	1174:1212	the CMC/ALG binary blend polymer wafers	1174:1212	These findings established that the CMC/ALG binary blend polymer wafers have the potential to improve the sublingual delivery and storage stability of protein-based vaccines.
33358974	7	1	contain	have	1214:1217	arg2	potential					1223:1231	the potential to improve the sublingual delivery and storage stability of protein-based vaccines	1219:1314	the potential to improve the sublingual delivery and storage stability of protein-based vaccines	1219:1314	These findings established that the CMC/ALG binary blend polymer wafers have the potential to improve the sublingual delivery and storage stability of protein-based vaccines.
33358974	6	2	theme	gp140	944:948	arg1	protein					950:956	HIV gp140 protein	940:956	HIV gp140 protein loaded in wafers of the optimal composition	940:1000	HIV gp140 protein loaded in wafers of the optimal composition could be stored and transported without cold chain, while maintaining antigen-specific immunogenicity after sublingual vaccination in mice.
33358974	5	3	theme	other	763:767	arg1	hand					769:772	the other hand	759:772	the other hand	759:772	On the other hand, wafers with high ALG content were not only mechanically robust, but also able to protect a model enzyme (β-galactosidase) against lyophilization and heat challenge.
33358974	2	4	theme	blends	346:351	arg1	series					321:326	a series	319:326	a series of binary polymer blends of carboxymethylcellulose (CMC) and alginate (ALG)	319:402	The wafers were composed of a series of binary polymer blends of carboxymethylcellulose (CMC) and alginate (ALG).
33358974	0	5	theme	protein	98:104	arg1	vaccines					106:113	protein vaccines	98:113	protein vaccines	98:113	Mucoadhesive wafers composed of binary polymer blends for sublingual delivery and preservation of protein vaccines.
33358974	3	6	theme	mechanical	493:502	arg1	properties					504:513	mechanical properties	493:513	mechanical properties	493:513	Varying the ratio between CMC and ALG resulted in wafers with different microstructure, mechanical properties, disintegration time, and release kinetics of model compounds.
33358974	5	7	theme	heat	924:927	arg1	challenge					929:937	heat challenge	924:937	heat challenge	924:937	On the other hand, wafers with high ALG content were not only mechanically robust, but also able to protect a model enzyme (β-galactosidase) against lyophilization and heat challenge.
33358974	4	8	theme	mucosal	646:652	arg1	tissue					654:659	sublingual mucosal tissue	635:659	sublingual mucosal tissue	635:659	Wafers with high CMC content were highly mucoadhesive to sublingual mucosal tissue and could withstand extensive washing, leading to improved protein permeation into the tissue.
33358974	7	9	theme	sublingual	1248:1257	arg1	delivery					1259:1266	sublingual delivery	1248:1266	sublingual delivery	1248:1266	These findings established that the CMC/ALG binary blend polymer wafers have the potential to improve the sublingual delivery and storage stability of protein-based vaccines.
33358974	7	10	dep	delivery	1259:1266	arg1	the					1244:1246	the	1244:1246	the	1244:1246	These findings established that the CMC/ALG binary blend polymer wafers have the potential to improve the sublingual delivery and storage stability of protein-based vaccines.
33358974	5	11	with	wafers	775:780	arg1	content					796:802	high ALG content	787:802	high ALG content	787:802	On the other hand, wafers with high ALG content were not only mechanically robust, but also able to protect a model enzyme (β-galactosidase) against lyophilization and heat challenge.
33358974	1	12	theme	protein	273:279	arg1	vaccines					281:288	protein vaccines	273:288	protein vaccines	273:288	The objective of this study is to develop a simple biopolymer platform of mucoadhesive wafers that enables effective sublingual delivery and preservation of protein vaccines.
33358974	5	13	theme	model	866:870	arg1	enzyme					872:877	a model enzyme	864:877	a model enzyme (β-galactosidase)	864:895	On the other hand, wafers with high ALG content were not only mechanically robust, but also able to protect a model enzyme (β-galactosidase) against lyophilization and heat challenge.
33358974	5	13	theme	model	866:870	arg1	β-galactosidase					880:894	β-galactosidase	880:894	β-galactosidase	880:894	On the other hand, wafers with high ALG content were not only mechanically robust, but also able to protect a model enzyme (β-galactosidase) against lyophilization and heat challenge.
33358974	6	14	theme	cold	1042:1045	arg1	chain					1047:1051	cold chain	1042:1051	cold chain	1042:1051	HIV gp140 protein loaded in wafers of the optimal composition could be stored and transported without cold chain, while maintaining antigen-specific immunogenicity after sublingual vaccination in mice.
33358974	6	15	theme	optimal	982:988	arg1	composition					990:1000	the optimal composition	978:1000	the optimal composition	978:1000	HIV gp140 protein loaded in wafers of the optimal composition could be stored and transported without cold chain, while maintaining antigen-specific immunogenicity after sublingual vaccination in mice.
33358974	2	16	theme	alginate	389:396	arg1	blends					346:351	binary polymer blends	331:351	binary polymer blends of carboxymethylcellulose (CMC) and alginate (ALG)	331:402	The wafers were composed of a series of binary polymer blends of carboxymethylcellulose (CMC) and alginate (ALG).
33358974	0	17	theme	vaccines	106:113	arg1	preservation					82:93	preservation	82:93	preservation of protein vaccines	82:113	Mucoadhesive wafers composed of binary polymer blends for sublingual delivery and preservation of protein vaccines.
33358974	0	17	theme	vaccines	106:113	arg1	delivery					69:76	sublingual delivery	58:76	sublingual delivery	58:76	Mucoadhesive wafers composed of binary polymer blends for sublingual delivery and preservation of protein vaccines.
33358974	3	18	with	wafers	455:460	arg1	time					531:534	disintegration time	516:534	disintegration time	516:534	Varying the ratio between CMC and ALG resulted in wafers with different microstructure, mechanical properties, disintegration time, and release kinetics of model compounds.
33358974	3	18	with	wafers	455:460	arg1	kinetics					549:556	release kinetics	541:556	release kinetics of model compounds	541:575	Varying the ratio between CMC and ALG resulted in wafers with different microstructure, mechanical properties, disintegration time, and release kinetics of model compounds.
33358974	3	18	with	wafers	455:460	arg1	microstructure					477:490	different microstructure	467:490	different microstructure	467:490	Varying the ratio between CMC and ALG resulted in wafers with different microstructure, mechanical properties, disintegration time, and release kinetics of model compounds.
33358974	3	18	with	wafers	455:460	arg1	properties					504:513	mechanical properties	493:513	mechanical properties	493:513	Varying the ratio between CMC and ALG resulted in wafers with different microstructure, mechanical properties, disintegration time, and release kinetics of model compounds.
33358974	7	19	theme	vaccines	1307:1314	arg1	delivery					1259:1266	sublingual delivery	1248:1266	sublingual delivery	1248:1266	These findings established that the CMC/ALG binary blend polymer wafers have the potential to improve the sublingual delivery and storage stability of protein-based vaccines.
33358974	7	19	theme	vaccines	1307:1314	arg1	stability					1280:1288	storage stability	1272:1288	storage stability	1272:1288	These findings established that the CMC/ALG binary blend polymer wafers have the potential to improve the sublingual delivery and storage stability of protein-based vaccines.
33358974	3	20	theme	disintegration	516:529	arg1	time					531:534	disintegration time	516:534	disintegration time	516:534	Varying the ratio between CMC and ALG resulted in wafers with different microstructure, mechanical properties, disintegration time, and release kinetics of model compounds.
33358974	1	21	theme	simple	160:165	arg1	platform					178:185	a simple biopolymer platform	158:185	a simple biopolymer platform of mucoadhesive wafers that enables effective sublingual delivery and preservation of protein vaccines	158:288	The objective of this study is to develop a simple biopolymer platform of mucoadhesive wafers that enables effective sublingual delivery and preservation of protein vaccines.
33358974	6	22	theme	HIV	940:942	arg1	protein					950:956	HIV gp140 protein	940:956	HIV gp140 protein loaded in wafers of the optimal composition	940:1000	HIV gp140 protein loaded in wafers of the optimal composition could be stored and transported without cold chain, while maintaining antigen-specific immunogenicity after sublingual vaccination in mice.
33358974	1	23	theme	biopolymer	167:176	arg1	platform					178:185	a simple biopolymer platform	158:185	a simple biopolymer platform of mucoadhesive wafers that enables effective sublingual delivery and preservation of protein vaccines	158:288	The objective of this study is to develop a simple biopolymer platform of mucoadhesive wafers that enables effective sublingual delivery and preservation of protein vaccines.
33358974	0	24	theme	Mucoadhesive	0:11	arg1	wafers					13:18	Mucoadhesive wafers	0:18	Mucoadhesive wafers	0:18	Mucoadhesive wafers composed of binary polymer blends for sublingual delivery and preservation of protein vaccines.
33358974	1	25	theme	vaccines	281:288	arg1	preservation					257:268	preservation	257:268	preservation of protein vaccines	257:288	The objective of this study is to develop a simple biopolymer platform of mucoadhesive wafers that enables effective sublingual delivery and preservation of protein vaccines.
33358974	1	25	theme	vaccines	281:288	arg1	delivery					244:251	effective sublingual delivery	223:251	effective sublingual delivery	223:251	The objective of this study is to develop a simple biopolymer platform of mucoadhesive wafers that enables effective sublingual delivery and preservation of protein vaccines.
33358974	4	26	theme	protein	720:726	arg1	permeation					728:737	improved protein permeation	711:737	improved protein permeation into the tissue	711:753	Wafers with high CMC content were highly mucoadhesive to sublingual mucosal tissue and could withstand extensive washing, leading to improved protein permeation into the tissue.
33358974	5	27	theme	ALG	792:794	arg1	content					796:802	high ALG content	787:802	high ALG content	787:802	On the other hand, wafers with high ALG content were not only mechanically robust, but also able to protect a model enzyme (β-galactosidase) against lyophilization and heat challenge.
33358974	5	28	theme	high	787:790	arg1	content					796:802	high ALG content	787:802	high ALG content	787:802	On the other hand, wafers with high ALG content were not only mechanically robust, but also able to protect a model enzyme (β-galactosidase) against lyophilization and heat challenge.
33358974	6	29	from	vaccination	1121:1131	arg1	mice					1136:1139	mice	1136:1139	mice	1136:1139	HIV gp140 protein loaded in wafers of the optimal composition could be stored and transported without cold chain, while maintaining antigen-specific immunogenicity after sublingual vaccination in mice.
33358974	7	30	theme	protein-based	1293:1305	arg1	vaccines					1307:1314	protein-based vaccines	1293:1314	protein-based vaccines	1293:1314	These findings established that the CMC/ALG binary blend polymer wafers have the potential to improve the sublingual delivery and storage stability of protein-based vaccines.
33358974	3	31	theme	release	541:547	arg1	kinetics					549:556	release kinetics	541:556	release kinetics of model compounds	541:575	Varying the ratio between CMC and ALG resulted in wafers with different microstructure, mechanical properties, disintegration time, and release kinetics of model compounds.
33358974	1	32	theme	mucoadhesive	190:201	arg1	wafers					203:208	mucoadhesive wafers	190:208	mucoadhesive wafers	190:208	The objective of this study is to develop a simple biopolymer platform of mucoadhesive wafers that enables effective sublingual delivery and preservation of protein vaccines.
33358974	0	33	theme	polymer	39:45	arg1	blends					47:52	binary polymer blends	32:52	binary polymer blends for sublingual delivery and preservation of protein vaccines	32:113	Mucoadhesive wafers composed of binary polymer blends for sublingual delivery and preservation of protein vaccines.
33358974	3	34	theme	different	467:475	arg1	microstructure					477:490	different microstructure	467:490	different microstructure	467:490	Varying the ratio between CMC and ALG resulted in wafers with different microstructure, mechanical properties, disintegration time, and release kinetics of model compounds.
33358974	1	35	theme	wafers	203:208	arg1	platform					178:185	a simple biopolymer platform	158:185	a simple biopolymer platform of mucoadhesive wafers that enables effective sublingual delivery and preservation of protein vaccines	158:288	The objective of this study is to develop a simple biopolymer platform of mucoadhesive wafers that enables effective sublingual delivery and preservation of protein vaccines.
33358974	0	36	theme	binary	32:37	arg1	blends					47:52	binary polymer blends	32:52	binary polymer blends for sublingual delivery and preservation of protein vaccines	32:113	Mucoadhesive wafers composed of binary polymer blends for sublingual delivery and preservation of protein vaccines.
33358974	6	37	theme	composition	990:1000	arg1	wafers					968:973	wafers	968:973	wafers of the optimal composition	968:1000	HIV gp140 protein loaded in wafers of the optimal composition could be stored and transported without cold chain, while maintaining antigen-specific immunogenicity after sublingual vaccination in mice.
33358974	7	38	theme	binary	1186:1191	arg1	wafers					1207:1212	the CMC/ALG binary blend polymer wafers	1174:1212	the CMC/ALG binary blend polymer wafers	1174:1212	These findings established that the CMC/ALG binary blend polymer wafers have the potential to improve the sublingual delivery and storage stability of protein-based vaccines.
33358974	3	39	theme	model	561:565	arg1	compounds					567:575	model compounds	561:575	model compounds	561:575	Varying the ratio between CMC and ALG resulted in wafers with different microstructure, mechanical properties, disintegration time, and release kinetics of model compounds.
33358974	2	40	theme	polymer	338:344	arg1	blends					346:351	binary polymer blends	331:351	binary polymer blends of carboxymethylcellulose (CMC) and alginate (ALG)	331:402	The wafers were composed of a series of binary polymer blends of carboxymethylcellulose (CMC) and alginate (ALG).
33358974	7	41	theme	CMC/ALG	1178:1184	arg1	wafers					1207:1212	the CMC/ALG binary blend polymer wafers	1174:1212	the CMC/ALG binary blend polymer wafers	1174:1212	These findings established that the CMC/ALG binary blend polymer wafers have the potential to improve the sublingual delivery and storage stability of protein-based vaccines.
33358974	3	42	theme	compounds	567:575	arg1	time					531:534	disintegration time	516:534	disintegration time	516:534	Varying the ratio between CMC and ALG resulted in wafers with different microstructure, mechanical properties, disintegration time, and release kinetics of model compounds.
33358974	3	42	theme	compounds	567:575	arg1	kinetics					549:556	release kinetics	541:556	release kinetics of model compounds	541:575	Varying the ratio between CMC and ALG resulted in wafers with different microstructure, mechanical properties, disintegration time, and release kinetics of model compounds.
33358974	3	42	theme	compounds	567:575	arg1	microstructure					477:490	different microstructure	467:490	different microstructure	467:490	Varying the ratio between CMC and ALG resulted in wafers with different microstructure, mechanical properties, disintegration time, and release kinetics of model compounds.
33358974	3	42	theme	compounds	567:575	arg1	properties					504:513	mechanical properties	493:513	mechanical properties	493:513	Varying the ratio between CMC and ALG resulted in wafers with different microstructure, mechanical properties, disintegration time, and release kinetics of model compounds.
33358974	7	43	theme	blend	1193:1197	arg1	wafers					1207:1212	the CMC/ALG binary blend polymer wafers	1174:1212	the CMC/ALG binary blend polymer wafers	1174:1212	These findings established that the CMC/ALG binary blend polymer wafers have the potential to improve the sublingual delivery and storage stability of protein-based vaccines.
33358974	2	44	theme	binary	331:336	arg1	blends					346:351	binary polymer blends	331:351	binary polymer blends of carboxymethylcellulose (CMC) and alginate (ALG)	331:402	The wafers were composed of a series of binary polymer blends of carboxymethylcellulose (CMC) and alginate (ALG).
33358974	4	45	with	Wafers	578:583	arg1	content					599:605	high CMC content	590:605	high CMC content	590:605	Wafers with high CMC content were highly mucoadhesive to sublingual mucosal tissue and could withstand extensive washing, leading to improved protein permeation into the tissue.
33358974	4	46	theme	improved	711:718	arg1	permeation					728:737	improved protein permeation	711:737	improved protein permeation into the tissue	711:753	Wafers with high CMC content were highly mucoadhesive to sublingual mucosal tissue and could withstand extensive washing, leading to improved protein permeation into the tissue.
33358974	4	47	theme	high	590:593	arg1	content					599:605	high CMC content	590:605	high CMC content	590:605	Wafers with high CMC content were highly mucoadhesive to sublingual mucosal tissue and could withstand extensive washing, leading to improved protein permeation into the tissue.
33358974	6	48	theme	antigen-specific	1072:1087	arg1	immunogenicity					1089:1102	antigen-specific immunogenicity	1072:1102	antigen-specific immunogenicity	1072:1102	HIV gp140 protein loaded in wafers of the optimal composition could be stored and transported without cold chain, while maintaining antigen-specific immunogenicity after sublingual vaccination in mice.
33358974	6	49	theme	sublingual	1110:1119	arg1	vaccination					1121:1131	sublingual vaccination	1110:1131	sublingual vaccination in mice	1110:1139	HIV gp140 protein loaded in wafers of the optimal composition could be stored and transported without cold chain, while maintaining antigen-specific immunogenicity after sublingual vaccination in mice.
33358974	1	50	theme	effective	223:231	arg1	delivery					244:251	effective sublingual delivery	223:251	effective sublingual delivery	223:251	The objective of this study is to develop a simple biopolymer platform of mucoadhesive wafers that enables effective sublingual delivery and preservation of protein vaccines.
33358974	1	51	theme	study	138:142	arg1	objective					120:128	The objective	116:128	The objective of this study	116:142	The objective of this study is to develop a simple biopolymer platform of mucoadhesive wafers that enables effective sublingual delivery and preservation of protein vaccines.
33358974	4	52	theme	sublingual	635:644	arg1	tissue					654:659	sublingual mucosal tissue	635:659	sublingual mucosal tissue	635:659	Wafers with high CMC content were highly mucoadhesive to sublingual mucosal tissue and could withstand extensive washing, leading to improved protein permeation into the tissue.
33358974	1	53	theme	sublingual	233:242	arg1	delivery					244:251	effective sublingual delivery	223:251	effective sublingual delivery	223:251	The objective of this study is to develop a simple biopolymer platform of mucoadhesive wafers that enables effective sublingual delivery and preservation of protein vaccines.
33358974	0	54	theme	sublingual	58:67	arg1	delivery					69:76	sublingual delivery	58:76	sublingual delivery	58:76	Mucoadhesive wafers composed of binary polymer blends for sublingual delivery and preservation of protein vaccines.
33358974	7	55	theme	storage	1272:1278	arg1	stability					1280:1288	storage stability	1272:1288	storage stability	1272:1288	These findings established that the CMC/ALG binary blend polymer wafers have the potential to improve the sublingual delivery and storage stability of protein-based vaccines.
33358974	2	56	theme	carboxymethylcellulose	356:377	arg1	blends					346:351	binary polymer blends	331:351	binary polymer blends of carboxymethylcellulose (CMC) and alginate (ALG)	331:402	The wafers were composed of a series of binary polymer blends of carboxymethylcellulose (CMC) and alginate (ALG).
33358974	4	57	theme	CMC	595:597	arg1	content					599:605	high CMC content	590:605	high CMC content	590:605	Wafers with high CMC content were highly mucoadhesive to sublingual mucosal tissue and could withstand extensive washing, leading to improved protein permeation into the tissue.
32825570	12	0	theme	topical	1478:1484	arg1	patches					1486:1492	local topical patches	1472:1492	local topical patches for wound care, periodontitis and oral cancer treatment	1472:1548	These versatile films can be further developed to achieve desirable characteristics for local topical patches for wound care, periodontitis and oral cancer treatment.
32825570	8	1	located	found	936:940	arg2	time					927:930	In vitro mucoadhesion time	905:930	In vitro mucoadhesion time	905:930	In vitro mucoadhesion time was found in the range of 0.5-6 h with porcine mucosa as model membrane under artificial saliva medium.
32825570	8	1	located	found	936:940	arg1	range					949:953	the range	945:953	the range of 0.5-6 h with porcine mucosa as model membrane under artificial saliva medium	945:1033	In vitro mucoadhesion time was found in the range of 0.5-6 h with porcine mucosa as model membrane under artificial saliva medium.
32825570	11	2	theme	skin	1365:1368	arg1	applications					1370:1381	leave-on skin applications	1356:1381	leave-on skin applications	1356:1381	Therefore, these curcumin-loaded films showed their potential for use as leave-on skin applications.
32825570	5	3	theme	No	621:622	arg1	release					624:630	No release	621:630	No release of curcumin from the films	621:657	No release of curcumin from the films was observed during the immersion in PBS and artificial saliva, and the fluid uptakes were in the range of 100-700%.
32825570	8	4	theme	In	905:906	arg1	time					927:930	In vitro mucoadhesion time	905:930	In vitro mucoadhesion time	905:930	In vitro mucoadhesion time was found in the range of 0.5-6 h with porcine mucosa as model membrane under artificial saliva medium.
32825570	1	5	theme	mechanical	325:334	arg1	blending					336:343	a simple, facile, cost-effective mechanical blending	292:343	a simple, facile, cost-effective mechanical blending	292:343	Multifunctional biopolymer composites comprising mechanically-disintegrated bacterial cellulose, alginate, gelatin and curcumin plasticized with glycerol were successfully fabricated through a simple, facile, cost-effective mechanical blending and casting method.
32825570	4	6	theme	commercial	591:600	arg1	products					611:618	commercial dressing products	591:618	commercial dressing products	591:618	Measured water vapor permeability values were 300-800 g/m2/24 h, which were comparable with those of commercial dressing products.
32825570	11	7	theme	leave-on	1356:1363	arg1	applications					1370:1381	leave-on skin applications	1356:1381	leave-on skin applications	1356:1381	Therefore, these curcumin-loaded films showed their potential for use as leave-on skin applications.
32825570	3	8	theme	contact	443:449	arg1	angles					451:456	The water contact angles	433:456	The water contact angles	433:456	The water contact angles existed in the range of 50-70°.
32825570	9	9	contain	had	1062:1064	arg1	films					1056:1060	The curcumin-loaded films	1036:1060	The curcumin-loaded films	1036:1060	The curcumin-loaded films had substantial antibacterial activity against E. coli and S. aureus.
32825570	9	9	contain	had	1062:1064	arg2	activity					1092:1099	substantial antibacterial activity	1066:1099	substantial antibacterial activity	1066:1099	The curcumin-loaded films had substantial antibacterial activity against E. coli and S. aureus.
32825570	8	10	theme	saliva	1021:1026	arg1	medium					1028:1033	artificial saliva medium	1010:1033	artificial saliva medium	1010:1033	In vitro mucoadhesion time was found in the range of 0.5-6 h with porcine mucosa as model membrane under artificial saliva medium.
32825570	9	11	theme	substantial	1066:1076	arg1	activity					1092:1099	substantial antibacterial activity	1066:1099	substantial antibacterial activity	1066:1099	The curcumin-loaded films had substantial antibacterial activity against E. coli and S. aureus.
32825570	8	12	theme	mucoadhesion	914:925	arg1	time					927:930	In vitro mucoadhesion time	905:930	In vitro mucoadhesion time	905:930	In vitro mucoadhesion time was found in the range of 0.5-6 h with porcine mucosa as model membrane under artificial saliva medium.
32825570	11	13	theme	curcumin-loaded	1300:1314	arg1	films					1316:1320	these curcumin-loaded films	1294:1320	these curcumin-loaded films	1294:1320	Therefore, these curcumin-loaded films showed their potential for use as leave-on skin applications.
32825570	5	14	theme	fluid	731:735	arg1	uptakes					737:743	the fluid uptakes	727:743	the fluid uptakes	727:743	No release of curcumin from the films was observed during the immersion in PBS and artificial saliva, and the fluid uptakes were in the range of 100-700%.
32825570	4	15	theme	Measured	490:497	arg1	values					524:529	Measured water vapor permeability values	490:529	Measured water vapor permeability values	490:529	Measured water vapor permeability values were 300-800 g/m2/24 h, which were comparable with those of commercial dressing products.
32825570	2	16	theme	SEM	365:367	arg1	images					369:374	SEM images	365:374	SEM images	365:374	SEM images indicate a well-distributed structure of the composites.
32825570	1	17	theme	casting	349:355	arg1	method					357:362	casting method	349:362	casting method	349:362	Multifunctional biopolymer composites comprising mechanically-disintegrated bacterial cellulose, alginate, gelatin and curcumin plasticized with glycerol were successfully fabricated through a simple, facile, cost-effective mechanical blending and casting method.
32825570	10	18	theme	human	1169:1173	arg1	keratinocytes					1175:1187	human keratinocytes	1169:1187	human keratinocytes	1169:1187	The films showed non-cytotoxicity to human keratinocytes and human gingival fibroblasts but exhibited potent anticancer activity in oral cancer cells.
32825570	1	19	theme	Multifunctional	101:115	arg1	composites					128:137	Multifunctional biopolymer composites	101:137	Multifunctional biopolymer composites comprising mechanically-disintegrated bacterial cellulose, alginate, gelatin and curcumin plasticized with glycerol	101:253	Multifunctional biopolymer composites comprising mechanically-disintegrated bacterial cellulose, alginate, gelatin and curcumin plasticized with glycerol were successfully fabricated through a simple, facile, cost-effective mechanical blending and casting method.
32825570	3	20	theme	50-70°	482:487	arg1	range					473:477	the range	469:477	the range of 50-70°	469:487	The water contact angles existed in the range of 50-70°.
32825570	12	21	theme	cancer	1533:1538	arg1	treatment					1540:1548	oral cancer treatment	1528:1548	oral cancer treatment	1528:1548	These versatile films can be further developed to achieve desirable characteristics for local topical patches for wound care, periodontitis and oral cancer treatment.
32825570	9	22	theme	curcumin-loaded	1040:1054	arg1	films					1056:1060	The curcumin-loaded films	1036:1060	The curcumin-loaded films	1036:1060	The curcumin-loaded films had substantial antibacterial activity against E. coli and S. aureus.
32825570	1	23	theme	biopolymer	117:126	arg1	composites					128:137	Multifunctional biopolymer composites	101:137	Multifunctional biopolymer composites comprising mechanically-disintegrated bacterial cellulose, alginate, gelatin and curcumin plasticized with glycerol	101:253	Multifunctional biopolymer composites comprising mechanically-disintegrated bacterial cellulose, alginate, gelatin and curcumin plasticized with glycerol were successfully fabricated through a simple, facile, cost-effective mechanical blending and casting method.
32825570	0	24	theme	Bacterial	16:24	arg1	Cellulose/Alginate/Gelatin					26:51	Bacterial Cellulose/Alginate/Gelatin	16:51	Bacterial Cellulose/Alginate/Gelatin	16:51	Curcumin-Loaded Bacterial Cellulose/Alginate/Gelatin as A Multifunctional Biopolymer Composite Film.
32825570	0	24	theme	Bacterial	16:24	arg1	Film					95:98	A Multifunctional Biopolymer Composite Film	56:98	A Multifunctional Biopolymer Composite Film	56:98	Curcumin-Loaded Bacterial Cellulose/Alginate/Gelatin as A Multifunctional Biopolymer Composite Film.
32825570	12	25	theme	oral	1528:1531	arg1	treatment					1540:1548	oral cancer treatment	1528:1548	oral cancer treatment	1528:1548	These versatile films can be further developed to achieve desirable characteristics for local topical patches for wound care, periodontitis and oral cancer treatment.
32825570	5	26	theme	artificial	704:713	arg1	saliva					715:720	artificial saliva	704:720	artificial saliva	704:720	No release of curcumin from the films was observed during the immersion in PBS and artificial saliva, and the fluid uptakes were in the range of 100-700%.
32825570	12	27	theme	local	1472:1476	arg1	patches					1486:1492	local topical patches	1472:1492	local topical patches for wound care, periodontitis and oral cancer treatment	1472:1548	These versatile films can be further developed to achieve desirable characteristics for local topical patches for wound care, periodontitis and oral cancer treatment.
32825570	12	28	theme	wound	1498:1502	arg1	care					1504:1507	wound care	1498:1507	wound care	1498:1507	These versatile films can be further developed to achieve desirable characteristics for local topical patches for wound care, periodontitis and oral cancer treatment.
32825570	8	29	with	range	949:953	arg1	mucosa					979:984	porcine mucosa	971:984	porcine mucosa as model membrane under artificial saliva medium	971:1033	In vitro mucoadhesion time was found in the range of 0.5-6 h with porcine mucosa as model membrane under artificial saliva medium.
32825570	6	30	theme	enduring	838:845	arg1	deformation					847:857	enduring deformation	838:857	enduring deformation	838:857	Films were stretchable and provided appropriate stiffness and enduring deformation.
32825570	2	31	theme	well-distributed	387:402	arg1	structure					404:412	a well-distributed structure	385:412	a well-distributed structure of the composites	385:430	SEM images indicate a well-distributed structure of the composites.
32825570	12	32	theme	versatile	1390:1398	arg1	films					1400:1404	These versatile films	1384:1404	These versatile films	1384:1404	These versatile films can be further developed to achieve desirable characteristics for local topical patches for wound care, periodontitis and oral cancer treatment.
32825570	10	33	theme	gingival	1199:1206	arg1	fibroblasts					1208:1218	human gingival fibroblasts	1193:1218	human gingival fibroblasts	1193:1218	The films showed non-cytotoxicity to human keratinocytes and human gingival fibroblasts but exhibited potent anticancer activity in oral cancer cells.
32825570	1	34	theme	mechanically-disintegrated	150:175	arg1	cellulose					187:195	mechanically-disintegrated bacterial cellulose	150:195	mechanically-disintegrated bacterial cellulose	150:195	Multifunctional biopolymer composites comprising mechanically-disintegrated bacterial cellulose, alginate, gelatin and curcumin plasticized with glycerol were successfully fabricated through a simple, facile, cost-effective mechanical blending and casting method.
32825570	7	35	theme	Hydrated	860:867	arg1	films					869:873	Hydrated films	860:873	Hydrated films	860:873	Hydrated films adhered firmly onto the skin.
32825570	1	36	theme	bacterial	177:185	arg1	cellulose					187:195	mechanically-disintegrated bacterial cellulose	150:195	mechanically-disintegrated bacterial cellulose	150:195	Multifunctional biopolymer composites comprising mechanically-disintegrated bacterial cellulose, alginate, gelatin and curcumin plasticized with glycerol were successfully fabricated through a simple, facile, cost-effective mechanical blending and casting method.
32825570	8	37	theme	h	964:964	arg1	range					949:953	the range	945:953	the range of 0.5-6 h with porcine mucosa as model membrane under artificial saliva medium	945:1033	In vitro mucoadhesion time was found in the range of 0.5-6 h with porcine mucosa as model membrane under artificial saliva medium.
32825570	5	38	from	immersion	683:691	arg1	PBS					696:698	PBS	696:698	PBS	696:698	No release of curcumin from the films was observed during the immersion in PBS and artificial saliva, and the fluid uptakes were in the range of 100-700%.
32825570	5	38	from	immersion	683:691	arg1	saliva					715:720	artificial saliva	704:720	artificial saliva	704:720	No release of curcumin from the films was observed during the immersion in PBS and artificial saliva, and the fluid uptakes were in the range of 100-700%.
32825570	0	39	theme	Multifunctional	58:72	arg1	Cellulose/Alginate/Gelatin					26:51	Bacterial Cellulose/Alginate/Gelatin	16:51	Bacterial Cellulose/Alginate/Gelatin	16:51	Curcumin-Loaded Bacterial Cellulose/Alginate/Gelatin as A Multifunctional Biopolymer Composite Film.
32825570	0	39	theme	Multifunctional	58:72	arg1	Film					95:98	A Multifunctional Biopolymer Composite Film	56:98	A Multifunctional Biopolymer Composite Film	56:98	Curcumin-Loaded Bacterial Cellulose/Alginate/Gelatin as A Multifunctional Biopolymer Composite Film.
32825570	10	40	theme	anticancer	1241:1250	arg1	activity					1252:1259	potent anticancer activity	1234:1259	potent anticancer activity	1234:1259	The films showed non-cytotoxicity to human keratinocytes and human gingival fibroblasts but exhibited potent anticancer activity in oral cancer cells.
32825570	5	41	theme	%	773:773	arg1	range					757:761	the range	753:761	the range of 100-700%	753:773	No release of curcumin from the films was observed during the immersion in PBS and artificial saliva, and the fluid uptakes were in the range of 100-700%.
32825570	8	42	theme	porcine	971:977	arg1	mucosa					979:984	porcine mucosa	971:984	porcine mucosa as model membrane under artificial saliva medium	971:1033	In vitro mucoadhesion time was found in the range of 0.5-6 h with porcine mucosa as model membrane under artificial saliva medium.
32825570	1	43	theme	simple	294:299	arg1	blending					336:343	a simple, facile, cost-effective mechanical blending	292:343	a simple, facile, cost-effective mechanical blending	292:343	Multifunctional biopolymer composites comprising mechanically-disintegrated bacterial cellulose, alginate, gelatin and curcumin plasticized with glycerol were successfully fabricated through a simple, facile, cost-effective mechanical blending and casting method.
32825570	0	44	theme	Composite	85:93	arg1	Cellulose/Alginate/Gelatin					26:51	Bacterial Cellulose/Alginate/Gelatin	16:51	Bacterial Cellulose/Alginate/Gelatin	16:51	Curcumin-Loaded Bacterial Cellulose/Alginate/Gelatin as A Multifunctional Biopolymer Composite Film.
32825570	0	44	theme	Composite	85:93	arg1	Film					95:98	A Multifunctional Biopolymer Composite Film	56:98	A Multifunctional Biopolymer Composite Film	56:98	Curcumin-Loaded Bacterial Cellulose/Alginate/Gelatin as A Multifunctional Biopolymer Composite Film.
32825570	4	45	theme	dressing	602:609	arg1	products					611:618	commercial dressing products	591:618	commercial dressing products	591:618	Measured water vapor permeability values were 300-800 g/m2/24 h, which were comparable with those of commercial dressing products.
32825570	6	46	theme	appropriate	812:822	arg1	stiffness					824:832	appropriate stiffness	812:832	appropriate stiffness	812:832	Films were stretchable and provided appropriate stiffness and enduring deformation.
32825570	0	47	theme	Biopolymer	74:83	arg1	Cellulose/Alginate/Gelatin					26:51	Bacterial Cellulose/Alginate/Gelatin	16:51	Bacterial Cellulose/Alginate/Gelatin	16:51	Curcumin-Loaded Bacterial Cellulose/Alginate/Gelatin as A Multifunctional Biopolymer Composite Film.
32825570	0	47	theme	Biopolymer	74:83	arg1	Film					95:98	A Multifunctional Biopolymer Composite Film	56:98	A Multifunctional Biopolymer Composite Film	56:98	Curcumin-Loaded Bacterial Cellulose/Alginate/Gelatin as A Multifunctional Biopolymer Composite Film.
32825570	3	48	theme	water	437:441	arg1	angles					451:456	The water contact angles	433:456	The water contact angles	433:456	The water contact angles existed in the range of 50-70°.
32825570	4	49	theme	vapor	505:509	arg1	values					524:529	Measured water vapor permeability values	490:529	Measured water vapor permeability values	490:529	Measured water vapor permeability values were 300-800 g/m2/24 h, which were comparable with those of commercial dressing products.
32825570	12	50	theme	desirable	1442:1450	arg1	characteristics					1452:1466	desirable characteristics	1442:1466	desirable characteristics	1442:1466	These versatile films can be further developed to achieve desirable characteristics for local topical patches for wound care, periodontitis and oral cancer treatment.
32825570	5	51	theme	curcumin	635:642	arg1	release					624:630	No release	621:630	No release of curcumin from the films	621:657	No release of curcumin from the films was observed during the immersion in PBS and artificial saliva, and the fluid uptakes were in the range of 100-700%.
32825570	2	52	theme	composites	421:430	arg1	structure					404:412	a well-distributed structure	385:412	a well-distributed structure of the composites	385:430	SEM images indicate a well-distributed structure of the composites.
32825570	9	53	theme	antibacterial	1078:1090	arg1	activity					1092:1099	substantial antibacterial activity	1066:1099	substantial antibacterial activity	1066:1099	The curcumin-loaded films had substantial antibacterial activity against E. coli and S. aureus.
32825570	4	54	theme	water	499:503	arg1	values					524:529	Measured water vapor permeability values	490:529	Measured water vapor permeability values	490:529	Measured water vapor permeability values were 300-800 g/m2/24 h, which were comparable with those of commercial dressing products.
32825570	8	55	dep	In	905:906	arg1	vitro					908:912	vitro	908:912	vitro	908:912	In vitro mucoadhesion time was found in the range of 0.5-6 h with porcine mucosa as model membrane under artificial saliva medium.
32825570	10	56	theme	cancer	1269:1274	arg1	cells					1276:1280	oral cancer cells	1264:1280	oral cancer cells	1264:1280	The films showed non-cytotoxicity to human keratinocytes and human gingival fibroblasts but exhibited potent anticancer activity in oral cancer cells.
32825570	10	57	theme	human	1193:1197	arg1	fibroblasts					1208:1218	human gingival fibroblasts	1193:1218	human gingival fibroblasts	1193:1218	The films showed non-cytotoxicity to human keratinocytes and human gingival fibroblasts but exhibited potent anticancer activity in oral cancer cells.
32825570	1	58	dep	simple	294:299	arg1	cost-effective					310:323	cost-effective	310:323	cost-effective	310:323	Multifunctional biopolymer composites comprising mechanically-disintegrated bacterial cellulose, alginate, gelatin and curcumin plasticized with glycerol were successfully fabricated through a simple, facile, cost-effective mechanical blending and casting method.
32825570	1	58	dep	simple	294:299	arg1	facile					302:307	facile	302:307	facile	302:307	Multifunctional biopolymer composites comprising mechanically-disintegrated bacterial cellulose, alginate, gelatin and curcumin plasticized with glycerol were successfully fabricated through a simple, facile, cost-effective mechanical blending and casting method.
32825570	5	59	from	films	653:657	arg1	release					624:630	No release	621:630	No release of curcumin from the films	621:657	No release of curcumin from the films was observed during the immersion in PBS and artificial saliva, and the fluid uptakes were in the range of 100-700%.
32825570	10	60	theme	oral	1264:1267	arg1	cells					1276:1280	oral cancer cells	1264:1280	oral cancer cells	1264:1280	The films showed non-cytotoxicity to human keratinocytes and human gingival fibroblasts but exhibited potent anticancer activity in oral cancer cells.
32825570	4	61	theme	permeability	511:522	arg1	values					524:529	Measured water vapor permeability values	490:529	Measured water vapor permeability values	490:529	Measured water vapor permeability values were 300-800 g/m2/24 h, which were comparable with those of commercial dressing products.
32825570	8	62	theme	model	989:993	arg1	membrane					995:1002	model membrane	989:1002	model membrane under artificial saliva medium	989:1033	In vitro mucoadhesion time was found in the range of 0.5-6 h with porcine mucosa as model membrane under artificial saliva medium.
32825570	4	63	with	comparable	566:575	arg1	those					582:586	those	582:586	those	582:586	Measured water vapor permeability values were 300-800 g/m2/24 h, which were comparable with those of commercial dressing products.
32825570	8	64	theme	artificial	1010:1019	arg1	medium					1028:1033	artificial saliva medium	1010:1033	artificial saliva medium	1010:1033	In vitro mucoadhesion time was found in the range of 0.5-6 h with porcine mucosa as model membrane under artificial saliva medium.
32825570	10	65	theme	potent	1234:1239	arg1	activity					1252:1259	potent anticancer activity	1234:1259	potent anticancer activity	1234:1259	The films showed non-cytotoxicity to human keratinocytes and human gingival fibroblasts but exhibited potent anticancer activity in oral cancer cells.
34959807	6	0	theme	cord	1215:1218	arg1	blood					1220:1224	GDM cord blood	1211:1224	GDM cord blood	1211:1224	(3) Results: 3'SL was higher in GDM cord blood.
34959807	0	1	from	Oligosaccharides	11:26	arg1	Blood					36:40	Cord Blood	31:40	Cord Blood	31:40	Human Milk Oligosaccharides in Cord Blood Are Altered in Gestational Diabetes and Stimulate Feto-Placental Angiogenesis In Vitro.
34959807	8	2	theme	spheroid	1411:1418	arg1	assays					1420:1425	spheroid assays	1411:1425	spheroid assays	1411:1425	In fibrin assays, HMOs and 3'SL increased total tube length by 24% and 25% (p < 0.05), in spheroid assays, by 32% (p < 0.05) and 21% (p = 0.056), respectively.
34959807	2	3	theme	cord	315:318	arg1	blood					320:324	fetal cord blood	309:324	fetal cord blood	309:324	HMOs are also in fetal cord blood and in contact with the feto-placental endothelium, potentially affecting its functions, such as angiogenesis.
34959807	10	4	theme	HMO	1624:1626	arg1	composition					1628:1638	HMO composition	1624:1638	HMO composition	1624:1638	(4) Conclusions: Our study suggests a novel role of HMOs in feto-placental angiogenesis and indicates a contribution of HMO composition to altered feto-placental vascularization in GDM.
34959807	8	5	theme	fibrin	1324:1329	arg1	assays					1331:1336	fibrin assays	1324:1336	fibrin assays	1324:1336	In fibrin assays, HMOs and 3'SL increased total tube length by 24% and 25% (p < 0.05), in spheroid assays, by 32% (p < 0.05) and 21% (p = 0.056), respectively.
34959807	10	6	dep	Conclusions	1508:1518	arg1	indicates					1596:1604	indicates	1596:1604	indicates a contribution of HMO composition to altered feto-placental vascularization in GDM	1596:1687	(4) Conclusions: Our study suggests a novel role of HMOs in feto-placental angiogenesis and indicates a contribution of HMO composition to altered feto-placental vascularization in GDM.
34959807	10	6	dep	Conclusions	1508:1518	arg1	suggests					1531:1538	suggests	1531:1538	suggests a novel role of HMOs in feto-placental angiogenesis	1531:1590	(4) Conclusions: Our study suggests a novel role of HMOs in feto-placental angiogenesis and indicates a contribution of HMO composition to altered feto-placental vascularization in GDM.
34959807	1	7	theme	Human	146:150	arg1	HMOs					175:178	HMOs	175:178	HMOs	175:178	(1) Background: Human milk oligosaccharides (HMOs) are present in maternal serum during pregnancy and their composition is altered in gestational diabetes (GDM).
34959807	1	7	theme	Human	146:150	arg1	oligosaccharides					157:172	Human milk oligosaccharides	146:172	Human milk oligosaccharides (HMOs)	146:179	(1) Background: Human milk oligosaccharides (HMOs) are present in maternal serum during pregnancy and their composition is altered in gestational diabetes (GDM).
34959807	5	8	theme	MTT	1040:1042	arg1	proliferation					1025:1037	proliferation	1025:1037	proliferation (MTT assays)	1025:1050	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	8	theme	MTT	1040:1042	arg1	assays					1044:1049	MTT assays	1040:1049	MTT assays	1040:1049	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	9	theme	network	989:995	arg1	formation					997:1005	network formation	989:1005	network formation (Matrigel assay)	989:1022	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	9	theme	network	989:995	arg1	assay					1017:1021	Matrigel assay	1008:1021	Matrigel assay	1008:1021	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	0	10	theme	Feto-Placental	92:105	arg1	Angiogenesis					107:118	Feto-Placental Angiogenesis	92:118	Feto-Placental Angiogenesis	92:118	Human Milk Oligosaccharides in Cord Blood Are Altered in Gestational Diabetes and Stimulate Feto-Placental Angiogenesis In Vitro.
34959807	4	11	with	women	628:632	arg1	tolerance					654:662	normal glucose tolerance	639:662	normal glucose tolerance (NGT, n = 25)	639:676	(2) Methods: Using HPLC, we quantified HMOs in cord blood of women with normal glucose tolerance (NGT, n = 25) or GDM (n = 26).
34959807	4	11	with	women	628:632	arg1	n					686:686	n = 26	686:691	n = 26	686:691	(2) Methods: Using HPLC, we quantified HMOs in cord blood of women with normal glucose tolerance (NGT, n = 25) or GDM (n = 26).
34959807	4	11	with	women	628:632	arg1	GDM					681:683	GDM	681:683	GDM (n = 26)	681:692	(2) Methods: Using HPLC, we quantified HMOs in cord blood of women with normal glucose tolerance (NGT, n = 25) or GDM (n = 26).
34959807	10	12	from	vascularization	1666:1680	arg1	GDM					1685:1687	GDM	1685:1687	GDM	1685:1687	(4) Conclusions: Our study suggests a novel role of HMOs in feto-placental angiogenesis and indicates a contribution of HMO composition to altered feto-placental vascularization in GDM.
34959807	1	13	dep	Background	134:143	arg1	altered					253:259	altered	253:259	is altered in gestational diabetes (GDM)	250:289	(1) Background: Human milk oligosaccharides (HMOs) are present in maternal serum during pregnancy and their composition is altered in gestational diabetes (GDM).
34959807	1	13	dep	Background	134:143	arg1	1					131:131	1	131:131	1	131:131	(1) Background: Human milk oligosaccharides (HMOs) are present in maternal serum during pregnancy and their composition is altered in gestational diabetes (GDM).
34959807	1	13	dep	Background	134:143	arg1	present					185:191	present	185:191	present	185:191	(1) Background: Human milk oligosaccharides (HMOs) are present in maternal serum during pregnancy and their composition is altered in gestational diabetes (GDM).
34959807	10	14	theme	HMOs	1556:1559	arg1	role					1548:1551	a novel role	1540:1551	a novel role of HMOs in feto-placental angiogenesis	1540:1590	(4) Conclusions: Our study suggests a novel role of HMOs in feto-placental angiogenesis and indicates a contribution of HMO composition to altered feto-placental vascularization in GDM.
34959807	5	15	theme	=	835:835	arg1	pregnancy					822:830	healthy pregnancy	814:830	healthy pregnancy (n = 10)	814:839	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	15	theme	=	835:835	arg1	n					833:833	n = 10	833:838	n = 10	833:838	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	3	16	theme	blood	463:467	arg1	HMOs					469:472	cord blood HMOs	458:472	cord blood HMOs	458:472	We hypothesized that cord blood HMOs are changed in GDM and contribute to increased feto-placental angiogenesis, hallmark of GDM.
34959807	4	17	dep	tolerance	654:662	arg1	=					672:672	=	672:672	=	672:672	(2) Methods: Using HPLC, we quantified HMOs in cord blood of women with normal glucose tolerance (NGT, n = 25) or GDM (n = 26).
34959807	8	18	theme	=	1457:1457	arg1	p					1455:1455	p = 0.056	1455:1463	p = 0.056	1455:1463	In fibrin assays, HMOs and 3'SL increased total tube length by 24% and 25% (p < 0.05), in spheroid assays, by 32% (p < 0.05) and 21% (p = 0.056), respectively.
34959807	8	18	theme	=	1457:1457	arg1	%					1452:1452	21%	1450:1452	21% (p = 0.056)	1450:1464	In fibrin assays, HMOs and 3'SL increased total tube length by 24% and 25% (p < 0.05), in spheroid assays, by 32% (p < 0.05) and 21% (p = 0.056), respectively.
34959807	5	19	theme	tube	1092:1095	arg1	assay					1130:1134	fibrin tube formation assay	1108:1134	fibrin tube formation assay	1108:1134	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	19	theme	tube	1092:1095	arg1	formation					1097:1105	tube formation	1092:1105	tube formation (fibrin tube formation assay)	1092:1135	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	1	20	theme	milk	152:155	arg1	HMOs					175:178	HMOs	175:178	HMOs	175:178	(1) Background: Human milk oligosaccharides (HMOs) are present in maternal serum during pregnancy and their composition is altered in gestational diabetes (GDM).
34959807	1	20	theme	milk	152:155	arg1	oligosaccharides					157:172	Human milk oligosaccharides	146:172	Human milk oligosaccharides (HMOs)	146:179	(1) Background: Human milk oligosaccharides (HMOs) are present in maternal serum during pregnancy and their composition is altered in gestational diabetes (GDM).
34959807	9	21	contain	had	1489:1491	arg2	effect					1496:1501	no effect	1493:1501	no effect	1493:1501	Lactose had no effect.
34959807	9	21	contain	had	1489:1491	arg1	Lactose					1481:1487	Lactose	1481:1487	Lactose	1481:1487	Lactose had no effect.
34959807	10	22	theme	feto-placental	1564:1577	arg1	angiogenesis					1579:1590	feto-placental angiogenesis	1564:1590	feto-placental angiogenesis	1564:1590	(4) Conclusions: Our study suggests a novel role of HMOs in feto-placental angiogenesis and indicates a contribution of HMO composition to altered feto-placental vascularization in GDM.
34959807	5	23	theme	spheroid	1152:1159	arg1	assay					1171:1175	spheroid sprouting assay	1152:1175	spheroid sprouting assay	1152:1175	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	23	theme	spheroid	1152:1159	arg1	sprouting					1141:1149	sprouting	1141:1149	sprouting (spheroid sprouting assay)	1141:1176	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	10	24	theme	novel	1542:1546	arg1	role					1548:1551	a novel role	1540:1551	a novel role of HMOs in feto-placental angiogenesis	1540:1590	(4) Conclusions: Our study suggests a novel role of HMOs in feto-placental angiogenesis and indicates a contribution of HMO composition to altered feto-placental vascularization in GDM.
34959807	10	25	theme	altered	1643:1649	arg1	vascularization					1666:1680	altered feto-placental vascularization	1643:1680	altered feto-placental vascularization in GDM	1643:1687	(4) Conclusions: Our study suggests a novel role of HMOs in feto-placental angiogenesis and indicates a contribution of HMO composition to altered feto-placental vascularization in GDM.
34959807	0	26	theme	Milk	6:9	arg1	Oligosaccharides					11:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides in Cord Blood	0:40	Human Milk Oligosaccharides in Cord Blood Are Altered in Gestational Diabetes and Stimulate Feto-Placental Angiogenesis In Vitro.
34959807	5	27	theme	vitro	714:718	arg1	SL					931:932	3'SL	929:932	3'SL	929:932	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	27	theme	vitro	714:718	arg1	angiogenesis					720:731	vitro angiogenesis	714:731	vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL)	714:943	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	28	theme	sprouting	1161:1169	arg1	assay					1171:1175	spheroid sprouting assay	1152:1175	spheroid sprouting assay	1152:1175	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	28	theme	sprouting	1161:1169	arg1	sprouting					1141:1149	sprouting	1141:1149	sprouting (spheroid sprouting assay)	1141:1176	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	3	29	theme	feto-placental	521:534	arg1	hallmark					550:557	hallmark	550:557	hallmark of GDM	550:564	We hypothesized that cord blood HMOs are changed in GDM and contribute to increased feto-placental angiogenesis, hallmark of GDM.
34959807	3	29	theme	feto-placental	521:534	arg1	angiogenesis					536:547	increased feto-placental angiogenesis	511:547	increased feto-placental angiogenesis	511:547	We hypothesized that cord blood HMOs are changed in GDM and contribute to increased feto-placental angiogenesis, hallmark of GDM.
34959807	5	30	theme	primary	739:745	arg1	fpECs					781:785	fpECs	781:785	fpECs	781:785	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	30	theme	primary	739:745	arg1	cells					774:778	primary feto-placental endothelial cells	739:778	primary feto-placental endothelial cells (fpECs)	739:786	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	0	31	theme	Human	0:4	arg1	Oligosaccharides					11:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides in Cord Blood	0:40	Human Milk Oligosaccharides in Cord Blood Are Altered in Gestational Diabetes and Stimulate Feto-Placental Angiogenesis In Vitro.
34959807	3	32	theme	increased	511:519	arg1	hallmark					550:557	hallmark	550:557	hallmark of GDM	550:564	We hypothesized that cord blood HMOs are changed in GDM and contribute to increased feto-placental angiogenesis, hallmark of GDM.
34959807	3	32	theme	increased	511:519	arg1	angiogenesis					536:547	increased feto-placental angiogenesis	511:547	increased feto-placental angiogenesis	511:547	We hypothesized that cord blood HMOs are changed in GDM and contribute to increased feto-placental angiogenesis, hallmark of GDM.
34959807	1	33	attach	present	185:191	arg2	HMOs					175:178	HMOs	175:178	HMOs	175:178	(1) Background: Human milk oligosaccharides (HMOs) are present in maternal serum during pregnancy and their composition is altered in gestational diabetes (GDM).
34959807	1	33	attach	present	185:191	arg1	serum					205:209	maternal serum	196:209	maternal serum during pregnancy	196:226	(1) Background: Human milk oligosaccharides (HMOs) are present in maternal serum during pregnancy and their composition is altered in gestational diabetes (GDM).
34959807	1	33	attach	present	185:191	arg2	oligosaccharides					157:172	Human milk oligosaccharides	146:172	Human milk oligosaccharides (HMOs)	146:179	(1) Background: Human milk oligosaccharides (HMOs) are present in maternal serum during pregnancy and their composition is altered in gestational diabetes (GDM).
34959807	5	34	theme	healthy	814:820	arg1	pregnancy					822:830	healthy pregnancy	814:830	healthy pregnancy (n = 10)	814:839	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	34	theme	healthy	814:820	arg1	n					833:833	n = 10	833:838	n = 10	833:838	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	35	theme	feto-placental	747:760	arg1	fpECs					781:785	fpECs	781:785	fpECs	781:785	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	35	theme	feto-placental	747:760	arg1	cells					774:778	primary feto-placental endothelial cells	739:778	primary feto-placental endothelial cells (fpECs)	739:786	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	4	36	dep	Methods	571:577	arg1	quantified					595:604	quantified	595:604	quantified HMOs in cord blood of women with normal glucose tolerance (NGT, n = 25) or GDM (n = 26)	595:692	(2) Methods: Using HPLC, we quantified HMOs in cord blood of women with normal glucose tolerance (NGT, n = 25) or GDM (n = 26).
34959807	4	36	dep	Methods	571:577	arg1	2					568:568	2	568:568	2	568:568	(2) Methods: Using HPLC, we quantified HMOs in cord blood of women with normal glucose tolerance (NGT, n = 25) or GDM (n = 26).
34959807	5	37	theme	F-actin	1073:1079	arg1	staining					1081:1088	F-actin staining	1073:1088	F-actin staining	1073:1088	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	37	theme	F-actin	1073:1079	arg1	organization					1059:1070	actin organization	1053:1070	actin organization (F-actin staining)	1053:1089	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	10	38	theme	feto-placental	1651:1664	arg1	vascularization					1666:1680	altered feto-placental vascularization	1643:1680	altered feto-placental vascularization in GDM	1643:1687	(4) Conclusions: Our study suggests a novel role of HMOs in feto-placental angiogenesis and indicates a contribution of HMO composition to altered feto-placental vascularization in GDM.
34959807	1	39	theme	gestational	264:274	arg1	GDM					286:288	GDM	286:288	GDM	286:288	(1) Background: Human milk oligosaccharides (HMOs) are present in maternal serum during pregnancy and their composition is altered in gestational diabetes (GDM).
34959807	1	39	theme	gestational	264:274	arg1	diabetes					276:283	gestational diabetes	264:283	gestational diabetes (GDM)	264:289	(1) Background: Human milk oligosaccharides (HMOs) are present in maternal serum during pregnancy and their composition is altered in gestational diabetes (GDM).
34959807	7	40	theme	network	1242:1248	arg1	formation					1250:1258	network formation	1242:1258	network formation	1242:1258	HMOs increased network formation, HMOs and 3'SL increased proliferation and F-actin staining.
34959807	2	41	theme	fetal	309:313	arg1	blood					320:324	fetal cord blood	309:324	fetal cord blood	309:324	HMOs are also in fetal cord blood and in contact with the feto-placental endothelium, potentially affecting its functions, such as angiogenesis.
34959807	5	42	dep	SL	931:932	arg1	µg/mL					938:942	30 µg/mL	935:942	30 µg/mL	935:942	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	43	theme	endothelial	762:772	arg1	fpECs					781:785	fpECs	781:785	fpECs	781:785	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	43	theme	endothelial	762:772	arg1	cells					774:778	primary feto-placental endothelial cells	739:778	primary feto-placental endothelial cells (fpECs)	739:786	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	4	44	theme	women	628:632	arg1	blood					619:623	cord blood	614:623	cord blood of women with normal glucose tolerance (NGT, n = 25) or GDM (n = 26)	614:692	(2) Methods: Using HPLC, we quantified HMOs in cord blood of women with normal glucose tolerance (NGT, n = 25) or GDM (n = 26).
34959807	10	45	from	role	1548:1551	arg1	angiogenesis					1579:1590	feto-placental angiogenesis	1564:1590	feto-placental angiogenesis	1564:1590	(4) Conclusions: Our study suggests a novel role of HMOs in feto-placental angiogenesis and indicates a contribution of HMO composition to altered feto-placental vascularization in GDM.
34959807	5	46	theme	human	899:903	arg1	milk					905:908	human milk	899:908	human milk	899:908	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	4	47	dep	=	672:672	arg1	NGT					665:667	NGT	665:667	NGT	665:667	(2) Methods: Using HPLC, we quantified HMOs in cord blood of women with normal glucose tolerance (NGT, n = 25) or GDM (n = 26).
34959807	6	48	theme	GDM	1211:1213	arg1	blood					1220:1224	GDM cord blood	1211:1224	GDM cord blood	1211:1224	(3) Results: 3'SL was higher in GDM cord blood.
34959807	5	49	attach	isolated	885:892	arg2	HMOs					868:871	HMOs	868:871	HMOs (100 µg/mL) isolated from human milk	868:908	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	49	attach	isolated	885:892	arg1	milk					905:908	human milk	899:908	human milk	899:908	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	49	attach	isolated	885:892	arg2	µg/mL					878:882	100 µg/mL	874:882	100 µg/mL	874:882	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	0	50	theme	Cord	31:34	arg1	Blood					36:40	Cord Blood	31:40	Cord Blood	31:40	Human Milk Oligosaccharides in Cord Blood Are Altered in Gestational Diabetes and Stimulate Feto-Placental Angiogenesis In Vitro.
34959807	3	51	theme	GDM	562:564	arg1	hallmark					550:557	hallmark	550:557	hallmark of GDM	550:564	We hypothesized that cord blood HMOs are changed in GDM and contribute to increased feto-placental angiogenesis, hallmark of GDM.
34959807	3	51	theme	GDM	562:564	arg1	angiogenesis					536:547	increased feto-placental angiogenesis	511:547	increased feto-placental angiogenesis	511:547	We hypothesized that cord blood HMOs are changed in GDM and contribute to increased feto-placental angiogenesis, hallmark of GDM.
34959807	1	52	from	present	185:191	arg1	serum					205:209	maternal serum	196:209	maternal serum during pregnancy	196:226	(1) Background: Human milk oligosaccharides (HMOs) are present in maternal serum during pregnancy and their composition is altered in gestational diabetes (GDM).
34959807	2	53	theme	feto-placental	350:363	arg1	endothelium					365:375	the feto-placental endothelium	346:375	the feto-placental endothelium	346:375	HMOs are also in fetal cord blood and in contact with the feto-placental endothelium, potentially affecting its functions, such as angiogenesis.
34959807	5	54	theme	HMOs	868:871	arg1	presence					845:852	presence	845:852	presence	845:852	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	54	theme	HMOs	868:871	arg1	absence					857:863	absence	857:863	absence	857:863	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	6	55	dep	Results	1183:1189	arg1	higher					1201:1206	higher	1201:1206	higher	1201:1206	(3) Results: 3'SL was higher in GDM cord blood.
34959807	8	56	theme	tube	1369:1372	arg1	length					1374:1379	total tube length	1363:1379	total tube length by 24%	1363:1386	In fibrin assays, HMOs and 3'SL increased total tube length by 24% and 25% (p < 0.05), in spheroid assays, by 32% (p < 0.05) and 21% (p = 0.056), respectively.
34959807	2	57	with	blood	320:324	arg1	endothelium					365:375	the feto-placental endothelium	346:375	the feto-placental endothelium	346:375	HMOs are also in fetal cord blood and in contact with the feto-placental endothelium, potentially affecting its functions, such as angiogenesis.
34959807	3	58	theme	cord	458:461	arg1	HMOs					469:472	cord blood HMOs	458:472	cord blood HMOs	458:472	We hypothesized that cord blood HMOs are changed in GDM and contribute to increased feto-placental angiogenesis, hallmark of GDM.
34959807	10	59	theme	composition	1628:1638	arg1	contribution					1608:1619	a contribution	1606:1619	a contribution of HMO composition to altered feto-placental vascularization in GDM	1606:1687	(4) Conclusions: Our study suggests a novel role of HMOs in feto-placental angiogenesis and indicates a contribution of HMO composition to altered feto-placental vascularization in GDM.
34959807	7	60	theme	F-actin	1303:1309	arg1	staining					1311:1318	F-actin staining	1303:1318	F-actin staining	1303:1318	HMOs increased network formation, HMOs and 3'SL increased proliferation and F-actin staining.
34959807	8	61	theme	p	1436:1436	arg1	%					1433:1433	32%	1431:1433	32% (p < 0.05)	1431:1444	In fibrin assays, HMOs and 3'SL increased total tube length by 24% and 25% (p < 0.05), in spheroid assays, by 32% (p < 0.05) and 21% (p = 0.056), respectively.
34959807	8	61	theme	p	1436:1436	arg1	<					1438:1438	p < 0.05	1436:1443	p < 0.05	1436:1443	In fibrin assays, HMOs and 3'SL increased total tube length by 24% and 25% (p < 0.05), in spheroid assays, by 32% (p < 0.05) and 21% (p = 0.056), respectively.
34959807	4	62	theme	=	688:688	arg1	n					686:686	n = 26	686:691	n = 26	686:691	(2) Methods: Using HPLC, we quantified HMOs in cord blood of women with normal glucose tolerance (NGT, n = 25) or GDM (n = 26).
34959807	4	62	theme	=	688:688	arg1	GDM					681:683	GDM	681:683	GDM (n = 26)	681:692	(2) Methods: Using HPLC, we quantified HMOs in cord blood of women with normal glucose tolerance (NGT, n = 25) or GDM (n = 26).
34959807	4	63	theme	glucose	646:652	arg1	tolerance					654:662	normal glucose tolerance	639:662	normal glucose tolerance (NGT, n = 25)	639:676	(2) Methods: Using HPLC, we quantified HMOs in cord blood of women with normal glucose tolerance (NGT, n = 25) or GDM (n = 26).
34959807	1	64	theme	maternal	196:203	arg1	serum					205:209	maternal serum	196:209	maternal serum during pregnancy	196:226	(1) Background: Human milk oligosaccharides (HMOs) are present in maternal serum during pregnancy and their composition is altered in gestational diabetes (GDM).
34959807	1	65	from	serum	205:209	arg1	present					185:191	present	185:191	present	185:191	(1) Background: Human milk oligosaccharides (HMOs) are present in maternal serum during pregnancy and their composition is altered in gestational diabetes (GDM).
34959807	5	66	theme	fibrin	1108:1113	arg1	assay					1130:1134	fibrin tube formation assay	1108:1134	fibrin tube formation assay	1108:1134	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	66	theme	fibrin	1108:1113	arg1	formation					1097:1105	tube formation	1092:1105	tube formation (fibrin tube formation assay)	1092:1135	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	4	67	theme	normal	639:644	arg1	tolerance					654:662	normal glucose tolerance	639:662	normal glucose tolerance (NGT, n = 25)	639:676	(2) Methods: Using HPLC, we quantified HMOs in cord blood of women with normal glucose tolerance (NGT, n = 25) or GDM (n = 26).
34959807	5	68	theme	actin	1053:1057	arg1	staining					1081:1088	F-actin staining	1073:1088	F-actin staining	1073:1088	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	68	theme	actin	1053:1057	arg1	organization					1059:1070	actin organization	1053:1070	actin organization (F-actin staining)	1053:1089	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	69	theme	term	793:796	arg1	placentas					798:806	term placentas	793:806	term placentas	793:806	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	0	70	theme	Gestational	57:67	arg1	Diabetes					69:76	Gestational Diabetes	57:76	Gestational Diabetes	57:76	Human Milk Oligosaccharides in Cord Blood Are Altered in Gestational Diabetes and Stimulate Feto-Placental Angiogenesis In Vitro.
34959807	1	71	located	present	185:191	arg2	HMOs					175:178	HMOs	175:178	HMOs	175:178	(1) Background: Human milk oligosaccharides (HMOs) are present in maternal serum during pregnancy and their composition is altered in gestational diabetes (GDM).
34959807	1	71	located	present	185:191	arg1	serum					205:209	maternal serum	196:209	maternal serum during pregnancy	196:226	(1) Background: Human milk oligosaccharides (HMOs) are present in maternal serum during pregnancy and their composition is altered in gestational diabetes (GDM).
34959807	1	71	located	present	185:191	arg2	oligosaccharides					157:172	Human milk oligosaccharides	146:172	Human milk oligosaccharides (HMOs)	146:179	(1) Background: Human milk oligosaccharides (HMOs) are present in maternal serum during pregnancy and their composition is altered in gestational diabetes (GDM).
34959807	5	72	theme	tube	1115:1118	arg1	assay					1130:1134	fibrin tube formation assay	1108:1134	fibrin tube formation assay	1108:1134	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	72	theme	tube	1115:1118	arg1	formation					1097:1105	tube formation	1092:1105	tube formation (fibrin tube formation assay)	1092:1135	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	4	73	theme	cord	614:617	arg1	blood					619:623	cord blood	614:623	cord blood of women with normal glucose tolerance (NGT, n = 25) or GDM (n = 26)	614:692	(2) Methods: Using HPLC, we quantified HMOs in cord blood of women with normal glucose tolerance (NGT, n = 25) or GDM (n = 26).
34959807	5	74	theme	glycan	958:963	arg1	lactose					949:955	lactose	949:955	lactose (glycan control)	949:972	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	74	theme	glycan	958:963	arg1	control					965:971	glycan control	958:971	glycan control	958:971	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	8	75	theme	total	1363:1367	arg1	length					1374:1379	total tube length	1363:1379	total tube length by 24%	1363:1386	In fibrin assays, HMOs and 3'SL increased total tube length by 24% and 25% (p < 0.05), in spheroid assays, by 32% (p < 0.05) and 21% (p = 0.056), respectively.
34959807	2	76	with	contact	333:339	arg1	endothelium					365:375	the feto-placental endothelium	346:375	the feto-placental endothelium	346:375	HMOs are also in fetal cord blood and in contact with the feto-placental endothelium, potentially affecting its functions, such as angiogenesis.
34959807	5	77	theme	formation	1120:1128	arg1	assay					1130:1134	fibrin tube formation assay	1108:1134	fibrin tube formation assay	1108:1134	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	77	theme	formation	1120:1128	arg1	formation					1097:1105	tube formation	1092:1105	tube formation (fibrin tube formation assay)	1092:1135	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	78	theme	Matrigel	1008:1015	arg1	formation					997:1005	network formation	989:1005	network formation (Matrigel assay)	989:1022	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	5	78	theme	Matrigel	1008:1015	arg1	assay					1017:1021	Matrigel assay	1008:1021	Matrigel assay	1008:1021	We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay).
34959807	8	79	theme	p	1397:1397	arg1	%					1394:1394	25%	1392:1394	25% (p < 0.05)	1392:1405	In fibrin assays, HMOs and 3'SL increased total tube length by 24% and 25% (p < 0.05), in spheroid assays, by 32% (p < 0.05) and 21% (p = 0.056), respectively.
34959807	8	79	theme	p	1397:1397	arg1	<					1399:1399	p < 0.05	1397:1404	p < 0.05	1397:1404	In fibrin assays, HMOs and 3'SL increased total tube length by 24% and 25% (p < 0.05), in spheroid assays, by 32% (p < 0.05) and 21% (p = 0.056), respectively.
34347566	8	0	theme	21°	1034:1036	arg1	angle					1019:1023	water contact angle	1005:1023	water contact angle (WCA) of 21°	1005:1036	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	8	0	theme	21°	1034:1036	arg1	capacity					983:990	water retention capacity	967:990	water retention capacity of 1101%	967:999	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	8	0	theme	21°	1034:1036	arg1	WCA					1026:1028	WCA	1026:1028	WCA	1026:1028	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	8	0	theme	21°	1034:1036	arg1	degree					950:955	the water swelling degree	931:955	the water swelling degree of 2004%	931:964	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	8	1	theme	contact	1011:1017	arg1	angle					1019:1023	water contact angle	1005:1023	water contact angle (WCA) of 21°	1005:1036	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	8	1	theme	contact	1011:1017	arg1	WCA					1026:1028	WCA	1026:1028	WCA	1026:1028	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	1	2	theme	polyelectrolyte	175:189	arg1	PCGO					200:203	PCGO	200:203	PCGO	200:203	Three-dimensional (3D) porous scaffolds based on graphene oxide (GO) incorporated pectin/chitosan polyelectrolyte complex (PCGO) were prepared by the freeze-drying technique.
34347566	1	2	theme	polyelectrolyte	175:189	arg1	complex					191:197	pectin/chitosan polyelectrolyte complex	159:197	pectin/chitosan polyelectrolyte complex (PCGO)	159:204	Three-dimensional (3D) porous scaffolds based on graphene oxide (GO) incorporated pectin/chitosan polyelectrolyte complex (PCGO) were prepared by the freeze-drying technique.
34347566	8	3	theme	water	1005:1009	arg1	angle					1019:1023	water contact angle	1005:1023	water contact angle (WCA) of 21°	1005:1036	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	8	3	theme	water	1005:1009	arg1	WCA					1026:1028	WCA	1026:1028	WCA	1026:1028	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	8	4	theme	water	967:971	arg1	capacity					983:990	water retention capacity	967:990	water retention capacity of 1101%	967:999	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	5	5	theme	PCGO	692:695	arg1	scaffolds					697:705	PCGO scaffolds	692:705	PCGO scaffolds	692:705	SEM analysis confirmed the uniform pore distribution of PCGO scaffolds.
34347566	2	6	theme	scaffolds	317:325	arg1	microstructure					281:294	microstructure	281:294	microstructure	281:294	The chemical composition and microstructure of the prepared PCGO scaffolds were studied by FTIR and XRD analysis.
34347566	2	6	theme	scaffolds	317:325	arg1	composition					265:275	chemical composition	256:275	chemical composition	256:275	The chemical composition and microstructure of the prepared PCGO scaffolds were studied by FTIR and XRD analysis.
34347566	10	7	theme	hemocompatibility	1231:1247	arg1	level					1214:1218	an acceptable level	1200:1218	an acceptable level of bio-and hemocompatibility	1200:1247	The PCGO scaffolds presented an acceptable level of bio-and hemocompatibility and GO concentration-dependent cell attachment ability.
34347566	4	8	theme	pectin/chitosan	611:625	arg1	complex					627:633	the pectin/chitosan complex	607:633	the pectin/chitosan complex	607:633	TGA analysis revealed that the addition of GO improves the thermal stability of the pectin/chitosan complex.
34347566	3	9	theme	GO	382:383	arg1	presence					370:377	The presence	366:377	The presence of GO	366:383	The presence of GO and its uniform distribution within the polymer matrix was confirmed by Raman spectroscopy and confocal Raman mapping analysis, respectively.
34347566	3	9	theme	GO	382:383	arg1	distribution					401:412	its uniform distribution	389:412	its uniform distribution within the polymer matrix	389:438	The presence of GO and its uniform distribution within the polymer matrix was confirmed by Raman spectroscopy and confocal Raman mapping analysis, respectively.
34347566	5	10	theme	scaffolds	697:705	arg1	distribution					676:687	the uniform pore distribution	659:687	the uniform pore distribution of PCGO scaffolds	659:705	SEM analysis confirmed the uniform pore distribution of PCGO scaffolds.
34347566	5	11	theme	SEM	636:638	arg1	analysis					640:647	SEM analysis	636:647	SEM analysis	636:647	SEM analysis confirmed the uniform pore distribution of PCGO scaffolds.
34347566	2	12	theme	PCGO	312:315	arg1	scaffolds					317:325	the prepared PCGO scaffolds	299:325	the prepared PCGO scaffolds	299:325	The chemical composition and microstructure of the prepared PCGO scaffolds were studied by FTIR and XRD analysis.
34347566	6	13	theme	pore	737:740	arg1	size					742:745	the pore size	733:745	the pore size of the scaffolds	733:762	Moreover, it showed that the pore size of the scaffolds was decreased with the increase in GO content.
34347566	10	14	theme	cell	1280:1283	arg1	ability					1296:1302	concentration-dependent cell attachment ability	1256:1302	concentration-dependent cell attachment ability	1256:1302	The PCGO scaffolds presented an acceptable level of bio-and hemocompatibility and GO concentration-dependent cell attachment ability.
34347566	3	15	theme	Raman	489:493	arg1	analysis					503:510	confocal Raman mapping analysis	480:510	confocal Raman mapping analysis	480:510	The presence of GO and its uniform distribution within the polymer matrix was confirmed by Raman spectroscopy and confocal Raman mapping analysis, respectively.
34347566	1	16	dep	oxide	135:139	arg1	GO					142:143	GO	142:143	GO	142:143	Three-dimensional (3D) porous scaffolds based on graphene oxide (GO) incorporated pectin/chitosan polyelectrolyte complex (PCGO) were prepared by the freeze-drying technique.
34347566	11	17	theme	tissue	1369:1374	arg1	engineering					1376:1386	tissue engineering	1369:1386	tissue engineering	1369:1386	These results demonstrate the suitability of PCGO scaffolds for tissue engineering.
34347566	10	18	theme	PCGO	1175:1178	arg1	scaffolds					1180:1188	The PCGO scaffolds	1171:1188	The PCGO scaffolds	1171:1188	The PCGO scaffolds presented an acceptable level of bio-and hemocompatibility and GO concentration-dependent cell attachment ability.
34347566	1	19	theme	porous	100:105	arg1	scaffolds					107:115	Three-dimensional (3D) porous scaffolds	77:115	Three-dimensional (3D) porous scaffolds based on graphene oxide (GO) incorporated pectin/chitosan polyelectrolyte complex (PCGO)	77:204	Three-dimensional (3D) porous scaffolds based on graphene oxide (GO) incorporated pectin/chitosan polyelectrolyte complex (PCGO) were prepared by the freeze-drying technique.
34347566	6	20	dep	content	802:808	arg1	GO					799:800	GO	799:800	GO	799:800	Moreover, it showed that the pore size of the scaffolds was decreased with the increase in GO content.
34347566	2	21	dep	composition	265:275	arg1	The					252:254	The	252:254	The	252:254	The chemical composition and microstructure of the prepared PCGO scaffolds were studied by FTIR and XRD analysis.
34347566	3	22	theme	uniform	393:399	arg1	distribution					401:412	its uniform distribution	389:412	its uniform distribution within the polymer matrix	389:438	The presence of GO and its uniform distribution within the polymer matrix was confirmed by Raman spectroscopy and confocal Raman mapping analysis, respectively.
34347566	9	23	attach	presented	1068:1076	arg1	addition					1042:1049	addition	1042:1049	addition	1042:1049	In addition, these scaffolds presented better compressive strength (∼283 kPa) and resistance towards lysozyme-mediated degradation.
34347566	9	23	attach	presented	1068:1076	arg2	scaffolds					1058:1066	these scaffolds	1052:1066	these scaffolds	1052:1066	In addition, these scaffolds presented better compressive strength (∼283 kPa) and resistance towards lysozyme-mediated degradation.
34347566	5	24	theme	uniform	663:669	arg1	distribution					676:687	the uniform pore distribution	659:687	the uniform pore distribution of PCGO scaffolds	659:705	SEM analysis confirmed the uniform pore distribution of PCGO scaffolds.
34347566	2	25	theme	FTIR	343:346	arg1	analysis					356:363	FTIR and XRD analysis	343:363	FTIR and XRD analysis	343:363	The chemical composition and microstructure of the prepared PCGO scaffolds were studied by FTIR and XRD analysis.
34347566	8	26	theme	GO	876:877	arg1	GO					876:877	GO	876:877	GO	876:877	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	8	26	theme	GO	876:877	arg1	%					871:871	%	871:871	% of GO	871:877	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	11	27	theme	scaffolds	1355:1363	arg1	suitability					1335:1345	the suitability	1331:1345	the suitability of PCGO scaffolds for tissue engineering	1331:1386	These results demonstrate the suitability of PCGO scaffolds for tissue engineering.
34347566	5	28	theme	pore	671:674	arg1	distribution					676:687	the uniform pore distribution	659:687	the uniform pore distribution of PCGO scaffolds	659:705	SEM analysis confirmed the uniform pore distribution of PCGO scaffolds.
34347566	8	29	theme	improved	893:900	arg1	hydrophilicity					902:915	the improved hydrophilicity	889:915	the improved hydrophilicity	889:915	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	0	30	theme	polyelectrolyte	42:56	arg1	scaffolds					66:74	pectin/chitosan polyelectrolyte complex scaffolds	26:74	pectin/chitosan polyelectrolyte complex scaffolds	26:74	Graphene oxide-reinforced pectin/chitosan polyelectrolyte complex scaffolds.
34347566	4	31	theme	GO	570:571	arg1	addition					558:565	the addition	554:565	the addition of GO	554:571	TGA analysis revealed that the addition of GO improves the thermal stability of the pectin/chitosan complex.
34347566	8	32	theme	retention	973:981	arg1	capacity					983:990	water retention capacity	967:990	water retention capacity of 1101%	967:999	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	9	33	theme	lysozyme-mediated	1140:1156	arg1	degradation					1158:1168	lysozyme-mediated degradation	1140:1168	lysozyme-mediated degradation	1140:1168	In addition, these scaffolds presented better compressive strength (∼283 kPa) and resistance towards lysozyme-mediated degradation.
34347566	3	34	theme	confocal	480:487	arg1	analysis					503:510	confocal Raman mapping analysis	480:510	confocal Raman mapping analysis	480:510	The presence of GO and its uniform distribution within the polymer matrix was confirmed by Raman spectroscopy and confocal Raman mapping analysis, respectively.
34347566	0	35	theme	pectin/chitosan	26:40	arg1	scaffolds					66:74	pectin/chitosan polyelectrolyte complex scaffolds	26:74	pectin/chitosan polyelectrolyte complex scaffolds	26:74	Graphene oxide-reinforced pectin/chitosan polyelectrolyte complex scaffolds.
34347566	4	36	theme	thermal	586:592	arg1	stability					594:602	the thermal stability	582:602	the thermal stability of the pectin/chitosan complex	582:633	TGA analysis revealed that the addition of GO improves the thermal stability of the pectin/chitosan complex.
34347566	2	37	theme	prepared	303:310	arg1	scaffolds					317:325	the prepared PCGO scaffolds	299:325	the prepared PCGO scaffolds	299:325	The chemical composition and microstructure of the prepared PCGO scaffolds were studied by FTIR and XRD analysis.
34347566	1	38	theme	graphene	126:133	arg1	oxide					135:139	graphene oxide	126:139	graphene oxide (GO) incorporated pectin/chitosan polyelectrolyte complex (PCGO)	126:204	Three-dimensional (3D) porous scaffolds based on graphene oxide (GO) incorporated pectin/chitosan polyelectrolyte complex (PCGO) were prepared by the freeze-drying technique.
34347566	7	39	theme	PCGO	831:834	arg1	scaffolds					836:844	the developed PCGO scaffolds	817:844	the developed PCGO scaffolds	817:844	Among the developed PCGO scaffolds, the scaffolds with 1 wt.
34347566	1	40	dep	Three-dimensional	77:93	arg1	3D					96:97	3D	96:97	3D	96:97	Three-dimensional (3D) porous scaffolds based on graphene oxide (GO) incorporated pectin/chitosan polyelectrolyte complex (PCGO) were prepared by the freeze-drying technique.
34347566	3	41	theme	polymer	425:431	arg1	matrix					433:438	the polymer matrix	421:438	the polymer matrix	421:438	The presence of GO and its uniform distribution within the polymer matrix was confirmed by Raman spectroscopy and confocal Raman mapping analysis, respectively.
34347566	2	42	theme	chemical	256:263	arg1	composition					265:275	chemical composition	256:275	chemical composition	256:275	The chemical composition and microstructure of the prepared PCGO scaffolds were studied by FTIR and XRD analysis.
34347566	9	43	theme	better	1078:1083	arg1	∼283 kPa					1107:1114	∼283 kPa	1107:1114	∼283 kPa	1107:1114	In addition, these scaffolds presented better compressive strength (∼283 kPa) and resistance towards lysozyme-mediated degradation.
34347566	9	43	theme	better	1078:1083	arg1	strength					1097:1104	better compressive strength	1078:1104	better compressive strength (∼283 kPa)	1078:1115	In addition, these scaffolds presented better compressive strength (∼283 kPa) and resistance towards lysozyme-mediated degradation.
34347566	0	44	theme	complex	58:64	arg1	scaffolds					66:74	pectin/chitosan polyelectrolyte complex scaffolds	26:74	pectin/chitosan polyelectrolyte complex scaffolds	26:74	Graphene oxide-reinforced pectin/chitosan polyelectrolyte complex scaffolds.
34347566	10	45	theme	bio-and	1223:1229	arg1	hemocompatibility					1231:1247	bio-and hemocompatibility	1223:1247	bio-and hemocompatibility	1223:1247	The PCGO scaffolds presented an acceptable level of bio-and hemocompatibility and GO concentration-dependent cell attachment ability.
34347566	9	46	theme	compressive	1085:1095	arg1	∼283 kPa					1107:1114	∼283 kPa	1107:1114	∼283 kPa	1107:1114	In addition, these scaffolds presented better compressive strength (∼283 kPa) and resistance towards lysozyme-mediated degradation.
34347566	9	46	theme	compressive	1085:1095	arg1	strength					1097:1104	better compressive strength	1078:1104	better compressive strength (∼283 kPa)	1078:1115	In addition, these scaffolds presented better compressive strength (∼283 kPa) and resistance towards lysozyme-mediated degradation.
34347566	1	47	theme	freeze-drying	227:239	arg1	technique					241:249	the freeze-drying technique	223:249	the freeze-drying technique	223:249	Three-dimensional (3D) porous scaffolds based on graphene oxide (GO) incorporated pectin/chitosan polyelectrolyte complex (PCGO) were prepared by the freeze-drying technique.
34347566	10	48	theme	attachment	1285:1294	arg1	ability					1296:1302	concentration-dependent cell attachment ability	1256:1302	concentration-dependent cell attachment ability	1256:1302	The PCGO scaffolds presented an acceptable level of bio-and hemocompatibility and GO concentration-dependent cell attachment ability.
34347566	7	49	theme	developed	821:829	arg1	scaffolds					836:844	the developed PCGO scaffolds	817:844	the developed PCGO scaffolds	817:844	Among the developed PCGO scaffolds, the scaffolds with 1 wt.
34347566	10	50	theme	acceptable	1203:1212	arg1	level					1214:1218	an acceptable level	1200:1218	an acceptable level of bio-and hemocompatibility	1200:1247	The PCGO scaffolds presented an acceptable level of bio-and hemocompatibility and GO concentration-dependent cell attachment ability.
34347566	4	51	theme	complex	627:633	arg1	stability					594:602	the thermal stability	582:602	the thermal stability of the pectin/chitosan complex	582:633	TGA analysis revealed that the addition of GO improves the thermal stability of the pectin/chitosan complex.
34347566	8	52	theme	swelling	941:948	arg1	degree					950:955	the water swelling degree	931:955	the water swelling degree of 2004%	931:964	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	6	53	from	increase	787:794	arg1	content					802:808	GO content	799:808	GO content	799:808	Moreover, it showed that the pore size of the scaffolds was decreased with the increase in GO content.
34347566	8	54	theme	%	999:999	arg1	angle					1019:1023	water contact angle	1005:1023	water contact angle (WCA) of 21°	1005:1036	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	8	54	theme	%	999:999	arg1	capacity					983:990	water retention capacity	967:990	water retention capacity of 1101%	967:999	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	8	54	theme	%	999:999	arg1	WCA					1026:1028	WCA	1026:1028	WCA	1026:1028	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	8	54	theme	%	999:999	arg1	degree					950:955	the water swelling degree	931:955	the water swelling degree of 2004%	931:964	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	8	55	theme	water	935:939	arg1	degree					950:955	the water swelling degree	931:955	the water swelling degree of 2004%	931:964	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	2	56	theme	XRD	352:354	arg1	analysis					356:363	FTIR and XRD analysis	343:363	FTIR and XRD analysis	343:363	The chemical composition and microstructure of the prepared PCGO scaffolds were studied by FTIR and XRD analysis.
34347566	11	57	theme	PCGO	1350:1353	arg1	scaffolds					1355:1363	PCGO scaffolds	1350:1363	PCGO scaffolds	1350:1363	These results demonstrate the suitability of PCGO scaffolds for tissue engineering.
34347566	6	58	theme	scaffolds	754:762	arg1	size					742:745	the pore size	733:745	the pore size of the scaffolds	733:762	Moreover, it showed that the pore size of the scaffolds was decreased with the increase in GO content.
34347566	3	59	theme	mapping	495:501	arg1	analysis					503:510	confocal Raman mapping analysis	480:510	confocal Raman mapping analysis	480:510	The presence of GO and its uniform distribution within the polymer matrix was confirmed by Raman spectroscopy and confocal Raman mapping analysis, respectively.
34347566	3	60	theme	Raman	457:461	arg1	spectroscopy					463:474	Raman spectroscopy	457:474	Raman spectroscopy	457:474	The presence of GO and its uniform distribution within the polymer matrix was confirmed by Raman spectroscopy and confocal Raman mapping analysis, respectively.
34347566	4	61	theme	TGA	527:529	arg1	analysis					531:538	TGA analysis	527:538	TGA analysis	527:538	TGA analysis revealed that the addition of GO improves the thermal stability of the pectin/chitosan complex.
34347566	1	62	theme	Three-dimensional	77:93	arg1	scaffolds					107:115	Three-dimensional (3D) porous scaffolds	77:115	Three-dimensional (3D) porous scaffolds based on graphene oxide (GO) incorporated pectin/chitosan polyelectrolyte complex (PCGO)	77:204	Three-dimensional (3D) porous scaffolds based on graphene oxide (GO) incorporated pectin/chitosan polyelectrolyte complex (PCGO) were prepared by the freeze-drying technique.
34347566	8	63	theme	%	964:964	arg1	angle					1019:1023	water contact angle	1005:1023	water contact angle (WCA) of 21°	1005:1036	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	8	63	theme	%	964:964	arg1	capacity					983:990	water retention capacity	967:990	water retention capacity of 1101%	967:999	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	8	63	theme	%	964:964	arg1	WCA					1026:1028	WCA	1026:1028	WCA	1026:1028	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	8	63	theme	%	964:964	arg1	degree					950:955	the water swelling degree	931:955	the water swelling degree of 2004%	931:964	% of GO presented the improved hydrophilicity by exhibiting the water swelling degree of 2004%, water retention capacity of 1101% and water contact angle (WCA) of 21°.
34347566	1	64	theme	pectin/chitosan	159:173	arg1	PCGO					200:203	PCGO	200:203	PCGO	200:203	Three-dimensional (3D) porous scaffolds based on graphene oxide (GO) incorporated pectin/chitosan polyelectrolyte complex (PCGO) were prepared by the freeze-drying technique.
34347566	1	64	theme	pectin/chitosan	159:173	arg1	complex					191:197	pectin/chitosan polyelectrolyte complex	159:197	pectin/chitosan polyelectrolyte complex (PCGO)	159:204	Three-dimensional (3D) porous scaffolds based on graphene oxide (GO) incorporated pectin/chitosan polyelectrolyte complex (PCGO) were prepared by the freeze-drying technique.
34347566	10	65	theme	concentration-dependent	1256:1278	arg1	ability					1296:1302	concentration-dependent cell attachment ability	1256:1302	concentration-dependent cell attachment ability	1256:1302	The PCGO scaffolds presented an acceptable level of bio-and hemocompatibility and GO concentration-dependent cell attachment ability.
33374249	0	0	theme	Enhanced	85:92	arg1	Applications					108:119	Enhanced Antimicrobial Applications	85:119	Enhanced Antimicrobial Applications	85:119	Rational Design of Ag/ZnO Hybrid Nanoparticles on Sericin/Agarose Composite Film for Enhanced Antimicrobial Applications.
33374249	6	1	theme	composite	1131:1139	arg1	film					1141:1144	the composite film	1127:1144	the composite film	1127:1144	In addition, the antibacterial activity of the composite film was evaluated against Gram-positive and Gram-negative bacteria.
33374249	6	2	theme	antibacterial	1101:1113	arg1	activity					1115:1122	the antibacterial activity	1097:1122	the antibacterial activity of the composite film	1097:1144	In addition, the antibacterial activity of the composite film was evaluated against Gram-positive and Gram-negative bacteria.
33374249	4	3	dep	composition	837:847	arg1	the					833:835	the	833:835	the	833:835	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively.
33374249	5	4	theme	absorption	1014:1023	arg1	ability					1025:1031	water absorption ability	1008:1031	water absorption ability	1008:1031	Water contact angle, swelling ratio, and mechanical property were determined to characterize the hydrophilicity, water absorption ability, and mechanical properties of the composite films.
33374249	0	5	from	Design	9:14	arg1	Film					76:79	Sericin/Agarose Composite Film	50:79	Sericin/Agarose Composite Film for Enhanced Antimicrobial Applications	50:119	Rational Design of Ag/ZnO Hybrid Nanoparticles on Sericin/Agarose Composite Film for Enhanced Antimicrobial Applications.
33374249	1	6	theme	potential	194:202	arg1	attention					181:189	attention	181:189	attention	181:189	Silver-based hybrid nanomaterials are receiving increasing attention as potential alternatives for traditional antimicrobial agents.
33374249	1	6	theme	potential	194:202	arg1	alternatives					204:215	potential alternatives	194:215	potential alternatives for traditional antimicrobial agents	194:252	Silver-based hybrid nanomaterials are receiving increasing attention as potential alternatives for traditional antimicrobial agents.
33374249	8	7	theme	great	1478:1482	arg1	biomaterial					1519:1529	a novel antimicrobial biomaterial	1497:1529	a novel antimicrobial biomaterial for wound dressing, artificial skin, and tissue engineering	1497:1589	It has shown great potential as a novel antimicrobial biomaterial for wound dressing, artificial skin, and tissue engineering.
33374249	8	7	theme	great	1478:1482	arg1	potential					1484:1492	great potential	1478:1492	great potential	1478:1492	It has shown great potential as a novel antimicrobial biomaterial for wound dressing, artificial skin, and tissue engineering.
33374249	4	8	theme	microscopy	642:651	arg1	spectroscopy					721:732	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy	624:732	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy	624:732	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively.
33374249	7	9	theme	desirable	1266:1274	arg1	hydrophilicity					1276:1289	desirable hydrophilicity	1266:1289	desirable hydrophilicity	1266:1289	The results showed that the composite film not only has desirable hydrophilicity, high water absorption ability, and favorable mechanical properties but also exhibits excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria.
33374249	7	10	theme	Gram-positive	1423:1435	arg1	bacteria					1455:1462	both Gram-positive and Gram-negative bacteria	1418:1462	both Gram-positive and Gram-negative bacteria	1418:1462	The results showed that the composite film not only has desirable hydrophilicity, high water absorption ability, and favorable mechanical properties but also exhibits excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria.
33374249	5	11	theme	films	1077:1081	arg1	hydrophilicity					992:1005	the hydrophilicity	988:1005	the hydrophilicity	988:1005	Water contact angle, swelling ratio, and mechanical property were determined to characterize the hydrophilicity, water absorption ability, and mechanical properties of the composite films.
33374249	5	11	theme	films	1077:1081	arg1	properties					1049:1058	mechanical properties	1038:1058	mechanical properties of the composite films	1038:1081	Water contact angle, swelling ratio, and mechanical property were determined to characterize the hydrophilicity, water absorption ability, and mechanical properties of the composite films.
33374249	5	11	theme	films	1077:1081	arg1	ability					1025:1031	water absorption ability	1008:1031	water absorption ability	1008:1031	Water contact angle, swelling ratio, and mechanical property were determined to characterize the hydrophilicity, water absorption ability, and mechanical properties of the composite films.
33374249	2	12	theme	oxide	337:341	arg1	ZnO					358:360	ZnO	358:360	ZnO	358:360	Here, we proposed a simple and eco-friendly strategy to efficiently assemble zinc oxide nanoparticles (ZnO) and silver nanoparticles (AgNPs) on sericin-agarose composite film to impart superior antimicrobial activity.
33374249	2	12	theme	oxide	337:341	arg1	nanoparticles					343:355	zinc oxide nanoparticles	332:355	zinc oxide nanoparticles (ZnO)	332:361	Here, we proposed a simple and eco-friendly strategy to efficiently assemble zinc oxide nanoparticles (ZnO) and silver nanoparticles (AgNPs) on sericin-agarose composite film to impart superior antimicrobial activity.
33374249	4	13	theme	composite	806:814	arg1	film					816:819	the composite film	802:819	the composite film	802:819	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively.
33374249	4	14	theme	X-ray	672:676	arg1	spectroscopy					678:689	energy-dispersive X-ray spectroscopy	654:689	energy-dispersive X-ray spectroscopy	654:689	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively.
33374249	0	15	theme	Antimicrobial	94:106	arg1	Applications					108:119	Enhanced Antimicrobial Applications	85:119	Enhanced Antimicrobial Applications	85:119	Rational Design of Ag/ZnO Hybrid Nanoparticles on Sericin/Agarose Composite Film for Enhanced Antimicrobial Applications.
33374249	2	16	theme	zinc	332:335	arg1	oxide					337:341	zinc oxide	332:341	zinc oxide nanoparticles (ZnO)	332:361	Here, we proposed a simple and eco-friendly strategy to efficiently assemble zinc oxide nanoparticles (ZnO) and silver nanoparticles (AgNPs) on sericin-agarose composite film to impart superior antimicrobial activity.
33374249	4	17	theme	energy-dispersive	654:670	arg1	spectroscopy					678:689	energy-dispersive X-ray spectroscopy	654:689	energy-dispersive X-ray spectroscopy	654:689	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively.
33374249	4	18	theme	AgNPs	770:774	arg1	morphology					756:765	the morphology	752:765	the morphology of AgNPs and ZnO on the surface of the composite film	752:819	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively.
33374249	7	19	theme	composite	1238:1246	arg1	film					1248:1251	the composite film	1234:1251	the composite film	1234:1251	The results showed that the composite film not only has desirable hydrophilicity, high water absorption ability, and favorable mechanical properties but also exhibits excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria.
33374249	5	20	theme	Water	895:899	arg1	angle					909:913	Water contact angle	895:913	Water contact angle	895:913	Water contact angle, swelling ratio, and mechanical property were determined to characterize the hydrophilicity, water absorption ability, and mechanical properties of the composite films.
33374249	7	21	theme	Gram-negative	1441:1453	arg1	bacteria					1455:1462	both Gram-positive and Gram-negative bacteria	1418:1462	both Gram-positive and Gram-negative bacteria	1418:1462	The results showed that the composite film not only has desirable hydrophilicity, high water absorption ability, and favorable mechanical properties but also exhibits excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria.
33374249	6	22	theme	film	1141:1144	arg1	activity					1115:1122	the antibacterial activity	1097:1122	the antibacterial activity of the composite film	1097:1144	In addition, the antibacterial activity of the composite film was evaluated against Gram-positive and Gram-negative bacteria.
33374249	6	23	theme	Gram-negative	1186:1198	arg1	bacteria					1200:1207	Gram-positive and Gram-negative bacteria	1168:1207	Gram-positive and Gram-negative bacteria	1168:1207	In addition, the antibacterial activity of the composite film was evaluated against Gram-positive and Gram-negative bacteria.
33374249	3	24	theme	adhesion	589:596	arg1	property					598:605	the adhesion property	585:605	the adhesion property of polydopamine	585:621	Based on a layer-by-layer self-assembly strategy, AgNPs and ZnO were immobilized on sericin-agarose films using the adhesion property of polydopamine.
33374249	3	25	theme	layer-by-layer	484:497	arg1	strategy					513:520	a layer-by-layer self-assembly strategy	482:520	a layer-by-layer self-assembly strategy	482:520	Based on a layer-by-layer self-assembly strategy, AgNPs and ZnO were immobilized on sericin-agarose films using the adhesion property of polydopamine.
33374249	7	26	theme	high	1292:1295	arg1	ability					1314:1320	high water absorption ability	1292:1320	high water absorption ability	1292:1320	The results showed that the composite film not only has desirable hydrophilicity, high water absorption ability, and favorable mechanical properties but also exhibits excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria.
33374249	1	27	theme	traditional	221:231	arg1	agents					247:252	traditional antimicrobial agents	221:252	traditional antimicrobial agents	221:252	Silver-based hybrid nanomaterials are receiving increasing attention as potential alternatives for traditional antimicrobial agents.
33374249	0	28	theme	Rational	0:7	arg1	Design					9:14	Rational Design	0:14	Rational Design of Ag/ZnO Hybrid Nanoparticles on Sericin/Agarose Composite Film for Enhanced Antimicrobial Applications.	0:120	Rational Design of Ag/ZnO Hybrid Nanoparticles on Sericin/Agarose Composite Film for Enhanced Antimicrobial Applications.
33374249	2	29	theme	composite	415:423	arg1	film					425:428	sericin-agarose composite film	399:428	sericin-agarose composite film to impart superior antimicrobial activity	399:470	Here, we proposed a simple and eco-friendly strategy to efficiently assemble zinc oxide nanoparticles (ZnO) and silver nanoparticles (AgNPs) on sericin-agarose composite film to impart superior antimicrobial activity.
33374249	0	30	theme	Ag/ZnO	19:24	arg1	Nanoparticles					33:45	Ag/ZnO Hybrid Nanoparticles	19:45	Ag/ZnO Hybrid Nanoparticles	19:45	Rational Design of Ag/ZnO Hybrid Nanoparticles on Sericin/Agarose Composite Film for Enhanced Antimicrobial Applications.
33374249	4	31	theme	AgNPs	866:870	arg1	structure					853:861	structure	853:861	structure	853:861	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively.
33374249	4	31	theme	AgNPs	866:870	arg1	composition					837:847	composition	837:847	composition	837:847	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively.
33374249	8	32	theme	novel	1499:1503	arg1	biomaterial					1519:1529	a novel antimicrobial biomaterial	1497:1529	a novel antimicrobial biomaterial for wound dressing, artificial skin, and tissue engineering	1497:1589	It has shown great potential as a novel antimicrobial biomaterial for wound dressing, artificial skin, and tissue engineering.
33374249	8	32	theme	novel	1499:1503	arg1	potential					1484:1492	great potential	1478:1492	great potential	1478:1492	It has shown great potential as a novel antimicrobial biomaterial for wound dressing, artificial skin, and tissue engineering.
33374249	3	33	theme	polydopamine	610:621	arg1	property					598:605	the adhesion property	585:605	the adhesion property of polydopamine	585:621	Based on a layer-by-layer self-assembly strategy, AgNPs and ZnO were immobilized on sericin-agarose films using the adhesion property of polydopamine.
33374249	4	34	theme	ZnO	876:878	arg1	structure					853:861	structure	853:861	structure	853:861	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively.
33374249	4	34	theme	ZnO	876:878	arg1	composition					837:847	composition	837:847	composition	837:847	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively.
33374249	4	35	theme	diffraction	709:719	arg1	spectroscopy					721:732	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy	624:732	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy	624:732	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively.
33374249	7	36	theme	excellent	1377:1385	arg1	activity					1401:1408	excellent antimicrobial activity	1377:1408	excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria	1377:1462	The results showed that the composite film not only has desirable hydrophilicity, high water absorption ability, and favorable mechanical properties but also exhibits excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria.
33374249	4	37	theme	electron	633:640	arg1	microscopy					642:651	Scanning electron microscopy	624:651	Scanning electron microscopy	624:651	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively.
33374249	4	38	used	used	739:742	arg2	spectroscopy					721:732	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy	624:732	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy	624:732	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively.
33374249	8	39	theme	artificial	1551:1560	arg1	skin					1562:1565	artificial skin	1551:1565	artificial skin	1551:1565	It has shown great potential as a novel antimicrobial biomaterial for wound dressing, artificial skin, and tissue engineering.
33374249	1	40	theme	Silver-based	122:133	arg1	nanomaterials					142:154	Silver-based hybrid nanomaterials	122:154	Silver-based hybrid nanomaterials	122:154	Silver-based hybrid nanomaterials are receiving increasing attention as potential alternatives for traditional antimicrobial agents.
33374249	4	41	theme	powder	702:707	arg1	diffraction					709:719	X-ray powder diffraction	696:719	X-ray powder diffraction	696:719	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively.
33374249	0	42	theme	Nanoparticles	33:45	arg1	Design					9:14	Rational Design	0:14	Rational Design of Ag/ZnO Hybrid Nanoparticles on Sericin/Agarose Composite Film for Enhanced Antimicrobial Applications.	0:120	Rational Design of Ag/ZnO Hybrid Nanoparticles on Sericin/Agarose Composite Film for Enhanced Antimicrobial Applications.
33374249	4	43	theme	Scanning	624:631	arg1	microscopy					642:651	Scanning electron microscopy	624:651	Scanning electron microscopy	624:651	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively.
33374249	3	44	theme	self-assembly	499:511	arg1	strategy					513:520	a layer-by-layer self-assembly strategy	482:520	a layer-by-layer self-assembly strategy	482:520	Based on a layer-by-layer self-assembly strategy, AgNPs and ZnO were immobilized on sericin-agarose films using the adhesion property of polydopamine.
33374249	2	45	theme	simple	275:280	arg1	strategy					299:306	a simple and eco-friendly strategy	273:306	a simple and eco-friendly strategy to efficiently assemble zinc oxide nanoparticles (ZnO) and silver nanoparticles (AgNPs) on sericin-agarose composite film to impart superior antimicrobial activity	273:470	Here, we proposed a simple and eco-friendly strategy to efficiently assemble zinc oxide nanoparticles (ZnO) and silver nanoparticles (AgNPs) on sericin-agarose composite film to impart superior antimicrobial activity.
33374249	1	46	theme	antimicrobial	233:245	arg1	agents					247:252	traditional antimicrobial agents	221:252	traditional antimicrobial agents	221:252	Silver-based hybrid nanomaterials are receiving increasing attention as potential alternatives for traditional antimicrobial agents.
33374249	2	47	theme	antimicrobial	449:461	arg1	activity					463:470	superior antimicrobial activity	440:470	superior antimicrobial activity	440:470	Here, we proposed a simple and eco-friendly strategy to efficiently assemble zinc oxide nanoparticles (ZnO) and silver nanoparticles (AgNPs) on sericin-agarose composite film to impart superior antimicrobial activity.
33374249	0	48	theme	Hybrid	26:31	arg1	Nanoparticles					33:45	Ag/ZnO Hybrid Nanoparticles	19:45	Ag/ZnO Hybrid Nanoparticles	19:45	Rational Design of Ag/ZnO Hybrid Nanoparticles on Sericin/Agarose Composite Film for Enhanced Antimicrobial Applications.
33374249	7	49	contain	has	1262:1264	arg2	hydrophilicity					1276:1289	desirable hydrophilicity	1266:1289	desirable hydrophilicity	1266:1289	The results showed that the composite film not only has desirable hydrophilicity, high water absorption ability, and favorable mechanical properties but also exhibits excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria.
33374249	7	49	contain	has	1262:1264	arg2	ability					1314:1320	high water absorption ability	1292:1320	high water absorption ability	1292:1320	The results showed that the composite film not only has desirable hydrophilicity, high water absorption ability, and favorable mechanical properties but also exhibits excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria.
33374249	7	49	contain	has	1262:1264	arg2	properties					1348:1357	favorable mechanical properties	1327:1357	favorable mechanical properties	1327:1357	The results showed that the composite film not only has desirable hydrophilicity, high water absorption ability, and favorable mechanical properties but also exhibits excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria.
33374249	7	49	contain	has	1262:1264	arg1	film					1248:1251	the composite film	1234:1251	the composite film	1234:1251	The results showed that the composite film not only has desirable hydrophilicity, high water absorption ability, and favorable mechanical properties but also exhibits excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria.
33374249	2	50	theme	superior	440:447	arg1	activity					463:470	superior antimicrobial activity	440:470	superior antimicrobial activity	440:470	Here, we proposed a simple and eco-friendly strategy to efficiently assemble zinc oxide nanoparticles (ZnO) and silver nanoparticles (AgNPs) on sericin-agarose composite film to impart superior antimicrobial activity.
33374249	0	51	theme	Sericin/Agarose	50:64	arg1	Film					76:79	Sericin/Agarose Composite Film	50:79	Sericin/Agarose Composite Film for Enhanced Antimicrobial Applications	50:119	Rational Design of Ag/ZnO Hybrid Nanoparticles on Sericin/Agarose Composite Film for Enhanced Antimicrobial Applications.
33374249	7	52	theme	absorption	1303:1312	arg1	ability					1314:1320	high water absorption ability	1292:1320	high water absorption ability	1292:1320	The results showed that the composite film not only has desirable hydrophilicity, high water absorption ability, and favorable mechanical properties but also exhibits excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria.
33374249	8	53	theme	antimicrobial	1505:1517	arg1	biomaterial					1519:1529	a novel antimicrobial biomaterial	1497:1529	a novel antimicrobial biomaterial for wound dressing, artificial skin, and tissue engineering	1497:1589	It has shown great potential as a novel antimicrobial biomaterial for wound dressing, artificial skin, and tissue engineering.
33374249	8	53	theme	antimicrobial	1505:1517	arg1	potential					1484:1492	great potential	1478:1492	great potential	1478:1492	It has shown great potential as a novel antimicrobial biomaterial for wound dressing, artificial skin, and tissue engineering.
33374249	6	54	theme	Gram-positive	1168:1180	arg1	bacteria					1200:1207	Gram-positive and Gram-negative bacteria	1168:1207	Gram-positive and Gram-negative bacteria	1168:1207	In addition, the antibacterial activity of the composite film was evaluated against Gram-positive and Gram-negative bacteria.
33374249	1	55	theme	hybrid	135:140	arg1	nanomaterials					142:154	Silver-based hybrid nanomaterials	122:154	Silver-based hybrid nanomaterials	122:154	Silver-based hybrid nanomaterials are receiving increasing attention as potential alternatives for traditional antimicrobial agents.
33374249	5	56	theme	water	1008:1012	arg1	ability					1025:1031	water absorption ability	1008:1031	water absorption ability	1008:1031	Water contact angle, swelling ratio, and mechanical property were determined to characterize the hydrophilicity, water absorption ability, and mechanical properties of the composite films.
33374249	8	57	theme	tissue	1572:1577	arg1	engineering					1579:1589	tissue engineering	1572:1589	tissue engineering	1572:1589	It has shown great potential as a novel antimicrobial biomaterial for wound dressing, artificial skin, and tissue engineering.
33374249	8	58	theme	wound	1535:1539	arg1	dressing					1541:1548	wound dressing	1535:1548	wound dressing	1535:1548	It has shown great potential as a novel antimicrobial biomaterial for wound dressing, artificial skin, and tissue engineering.
33374249	3	59	theme	sericin-agarose	557:571	arg1	films					573:577	sericin-agarose films	557:577	sericin-agarose films using the adhesion property of polydopamine	557:621	Based on a layer-by-layer self-assembly strategy, AgNPs and ZnO were immobilized on sericin-agarose films using the adhesion property of polydopamine.
33374249	4	60	theme	ZnO	780:782	arg1	morphology					756:765	the morphology	752:765	the morphology of AgNPs and ZnO on the surface of the composite film	752:819	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively.
33374249	5	61	theme	contact	901:907	arg1	angle					909:913	Water contact angle	895:913	Water contact angle	895:913	Water contact angle, swelling ratio, and mechanical property were determined to characterize the hydrophilicity, water absorption ability, and mechanical properties of the composite films.
33374249	2	62	theme	silver	367:372	arg1	nanoparticles					374:386	silver nanoparticles	367:386	silver nanoparticles (AgNPs)	367:394	Here, we proposed a simple and eco-friendly strategy to efficiently assemble zinc oxide nanoparticles (ZnO) and silver nanoparticles (AgNPs) on sericin-agarose composite film to impart superior antimicrobial activity.
33374249	2	62	theme	silver	367:372	arg1	AgNPs					389:393	AgNPs	389:393	AgNPs	389:393	Here, we proposed a simple and eco-friendly strategy to efficiently assemble zinc oxide nanoparticles (ZnO) and silver nanoparticles (AgNPs) on sericin-agarose composite film to impart superior antimicrobial activity.
33374249	4	63	theme	film	816:819	arg1	surface					791:797	the surface	787:797	the surface of the composite film	787:819	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively.
33374249	5	64	theme	mechanical	1038:1047	arg1	properties					1049:1058	mechanical properties	1038:1058	mechanical properties of the composite films	1038:1081	Water contact angle, swelling ratio, and mechanical property were determined to characterize the hydrophilicity, water absorption ability, and mechanical properties of the composite films.
33374249	7	65	theme	favorable	1327:1335	arg1	properties					1348:1357	favorable mechanical properties	1327:1357	favorable mechanical properties	1327:1357	The results showed that the composite film not only has desirable hydrophilicity, high water absorption ability, and favorable mechanical properties but also exhibits excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria.
33374249	4	66	theme	spectroscopy	678:689	arg1	spectroscopy					721:732	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy	624:732	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy	624:732	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively.
33374249	7	67	theme	water	1297:1301	arg1	ability					1314:1320	high water absorption ability	1292:1320	high water absorption ability	1292:1320	The results showed that the composite film not only has desirable hydrophilicity, high water absorption ability, and favorable mechanical properties but also exhibits excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria.
33374249	4	68	from	morphology	756:765	arg1	surface					791:797	the surface	787:797	the surface of the composite film	787:819	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively.
33374249	2	69	theme	eco-friendly	286:297	arg1	strategy					299:306	a simple and eco-friendly strategy	273:306	a simple and eco-friendly strategy to efficiently assemble zinc oxide nanoparticles (ZnO) and silver nanoparticles (AgNPs) on sericin-agarose composite film to impart superior antimicrobial activity	273:470	Here, we proposed a simple and eco-friendly strategy to efficiently assemble zinc oxide nanoparticles (ZnO) and silver nanoparticles (AgNPs) on sericin-agarose composite film to impart superior antimicrobial activity.
33374249	7	70	theme	antimicrobial	1387:1399	arg1	activity					1401:1408	excellent antimicrobial activity	1377:1408	excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria	1377:1462	The results showed that the composite film not only has desirable hydrophilicity, high water absorption ability, and favorable mechanical properties but also exhibits excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria.
33374249	2	71	theme	sericin-agarose	399:413	arg1	film					425:428	sericin-agarose composite film	399:428	sericin-agarose composite film to impart superior antimicrobial activity	399:470	Here, we proposed a simple and eco-friendly strategy to efficiently assemble zinc oxide nanoparticles (ZnO) and silver nanoparticles (AgNPs) on sericin-agarose composite film to impart superior antimicrobial activity.
33374249	5	72	theme	swelling	916:923	arg1	ratio					925:929	swelling ratio	916:929	swelling ratio	916:929	Water contact angle, swelling ratio, and mechanical property were determined to characterize the hydrophilicity, water absorption ability, and mechanical properties of the composite films.
33374249	4	73	theme	X-ray	696:700	arg1	diffraction					709:719	X-ray powder diffraction	696:719	X-ray powder diffraction	696:719	Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively.
33374249	0	74	theme	Composite	66:74	arg1	Film					76:79	Sericin/Agarose Composite Film	50:79	Sericin/Agarose Composite Film for Enhanced Antimicrobial Applications	50:119	Rational Design of Ag/ZnO Hybrid Nanoparticles on Sericin/Agarose Composite Film for Enhanced Antimicrobial Applications.
33374249	5	75	theme	composite	1067:1075	arg1	films					1077:1081	the composite films	1063:1081	the composite films	1063:1081	Water contact angle, swelling ratio, and mechanical property were determined to characterize the hydrophilicity, water absorption ability, and mechanical properties of the composite films.
33374249	7	76	theme	mechanical	1337:1346	arg1	properties					1348:1357	favorable mechanical properties	1327:1357	favorable mechanical properties	1327:1357	The results showed that the composite film not only has desirable hydrophilicity, high water absorption ability, and favorable mechanical properties but also exhibits excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria.
33374249	5	77	theme	mechanical	936:945	arg1	property					947:954	mechanical property	936:954	mechanical property	936:954	Water contact angle, swelling ratio, and mechanical property were determined to characterize the hydrophilicity, water absorption ability, and mechanical properties of the composite films.
34694716	0	0	theme	gum	96:98	arg1	nanocomposite					113:125	aminated gum acacia based nanocomposite	87:125	aminated gum acacia based nanocomposite	87:125	Antibiofilm and antimicrobial efficacy evaluation of polypyrrole nanotubes embedded in aminated gum acacia based nanocomposite.
34694716	4	1	theme	promising	965:973	arg1	performance					989:999	promising antimicrobial performance	965:999	promising antimicrobial performance	965:999	Interestingly, the prepared PPyNT@AGA nanocomposite exhibited 90% biofilm inhibition against gram-negative Pseudomonas aeruginosa, gram-positive Streptococcus pneumoniae and fungal strain Candida albicans with promising antimicrobial performance.
34694716	1	2	theme	hybrid	188:193	arg1	composites					195:204	natural polysaccharide-based hybrid composites	159:204	natural polysaccharide-based hybrid composites	159:204	The sustainable development of natural polysaccharide-based hybrid composites is highly important for the effective replacement of metal nanoparticles in diverse applications.
34694716	0	3	theme	aminated	87:94	arg1	nanocomposite					113:125	aminated gum acacia based nanocomposite	87:125	aminated gum acacia based nanocomposite	87:125	Antibiofilm and antimicrobial efficacy evaluation of polypyrrole nanotubes embedded in aminated gum acacia based nanocomposite.
34694716	2	4	theme	gum	382:384	arg1	acacia					386:391	aminated gum acacia	373:391	aminated gum acacia (AGA)	373:397	Here, polypyrrole nanotubes (PPyNTs) were embedded on the surface of aminated gum acacia (AGA) to produce ecofriendly nanocomposites for biomedical applications.
34694716	2	4	theme	gum	382:384	arg1	AGA					394:396	AGA	394:396	AGA	394:396	Here, polypyrrole nanotubes (PPyNTs) were embedded on the surface of aminated gum acacia (AGA) to produce ecofriendly nanocomposites for biomedical applications.
34694716	5	5	theme	various	1085:1091	arg1	microorganisms					1093:1106	various microorganisms	1085:1106	various microorganisms	1085:1106	This study establishes the good inhibition of a PPyNT@AGA hybrid composite against various microorganisms.
34694716	0	6	theme	based	107:111	arg1	nanocomposite					113:125	aminated gum acacia based nanocomposite	87:125	aminated gum acacia based nanocomposite	87:125	Antibiofilm and antimicrobial efficacy evaluation of polypyrrole nanotubes embedded in aminated gum acacia based nanocomposite.
34694716	3	7	theme	electron	562:569	arg1	microscopy					571:580	scanning electron microscopy	553:580	scanning electron microscopy	553:580	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite was studied by scanning electron microscopy and transmission electron microscopy and their affirmed interactions were characterised by X-ray diffraction, Raman, Fourier transform-infrared and UV-visible spectroscopy.
34694716	3	8	theme	transmission	586:597	arg1	microscopy					608:617	transmission electron microscopy	586:617	transmission electron microscopy	586:617	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite was studied by scanning electron microscopy and transmission electron microscopy and their affirmed interactions were characterised by X-ray diffraction, Raman, Fourier transform-infrared and UV-visible spectroscopy.
34694716	0	9	theme	acacia	100:105	arg1	nanocomposite					113:125	aminated gum acacia based nanocomposite	87:125	aminated gum acacia based nanocomposite	87:125	Antibiofilm and antimicrobial efficacy evaluation of polypyrrole nanotubes embedded in aminated gum acacia based nanocomposite.
34694716	4	10	theme	antimicrobial	975:987	arg1	performance					989:999	promising antimicrobial performance	965:999	promising antimicrobial performance	965:999	Interestingly, the prepared PPyNT@AGA nanocomposite exhibited 90% biofilm inhibition against gram-negative Pseudomonas aeruginosa, gram-positive Streptococcus pneumoniae and fungal strain Candida albicans with promising antimicrobial performance.
34694716	2	11	theme	acacia	386:391	arg1	surface					362:368	the surface	358:368	the surface of aminated gum acacia (AGA) to produce ecofriendly nanocomposites for biomedical applications	358:463	Here, polypyrrole nanotubes (PPyNTs) were embedded on the surface of aminated gum acacia (AGA) to produce ecofriendly nanocomposites for biomedical applications.
34694716	4	12	theme	PPyNT	783:787	arg1	nanocomposite					793:805	the prepared PPyNT@AGA nanocomposite	770:805	the prepared PPyNT@AGA nanocomposite	770:805	Interestingly, the prepared PPyNT@AGA nanocomposite exhibited 90% biofilm inhibition against gram-negative Pseudomonas aeruginosa, gram-positive Streptococcus pneumoniae and fungal strain Candida albicans with promising antimicrobial performance.
34694716	4	13	theme	prepared	774:781	arg1	nanocomposite					793:805	the prepared PPyNT@AGA nanocomposite	770:805	the prepared PPyNT@AGA nanocomposite	770:805	Interestingly, the prepared PPyNT@AGA nanocomposite exhibited 90% biofilm inhibition against gram-negative Pseudomonas aeruginosa, gram-positive Streptococcus pneumoniae and fungal strain Candida albicans with promising antimicrobial performance.
34694716	4	14	theme	strain	936:941	arg1	albicans					951:958	fungal strain Candida albicans	929:958	fungal strain Candida albicans	929:958	Interestingly, the prepared PPyNT@AGA nanocomposite exhibited 90% biofilm inhibition against gram-negative Pseudomonas aeruginosa, gram-positive Streptococcus pneumoniae and fungal strain Candida albicans with promising antimicrobial performance.
34694716	2	15	theme	biomedical	441:450	arg1	applications					452:463	biomedical applications	441:463	biomedical applications	441:463	Here, polypyrrole nanotubes (PPyNTs) were embedded on the surface of aminated gum acacia (AGA) to produce ecofriendly nanocomposites for biomedical applications.
34694716	2	16	theme	ecofriendly	410:420	arg1	nanocomposites					422:435	ecofriendly nanocomposites	410:435	ecofriendly nanocomposites for biomedical applications	410:463	Here, polypyrrole nanotubes (PPyNTs) were embedded on the surface of aminated gum acacia (AGA) to produce ecofriendly nanocomposites for biomedical applications.
34694716	4	17	theme	fungal	929:934	arg1	albicans					951:958	fungal strain Candida albicans	929:958	fungal strain Candida albicans	929:958	Interestingly, the prepared PPyNT@AGA nanocomposite exhibited 90% biofilm inhibition against gram-negative Pseudomonas aeruginosa, gram-positive Streptococcus pneumoniae and fungal strain Candida albicans with promising antimicrobial performance.
34694716	2	18	theme	polypyrrole	310:320	arg1	PPyNTs					333:338	PPyNTs	333:338	PPyNTs	333:338	Here, polypyrrole nanotubes (PPyNTs) were embedded on the surface of aminated gum acacia (AGA) to produce ecofriendly nanocomposites for biomedical applications.
34694716	2	18	theme	polypyrrole	310:320	arg1	nanotubes					322:330	polypyrrole nanotubes	310:330	polypyrrole nanotubes (PPyNTs)	310:339	Here, polypyrrole nanotubes (PPyNTs) were embedded on the surface of aminated gum acacia (AGA) to produce ecofriendly nanocomposites for biomedical applications.
34694716	3	19	theme	electron	599:606	arg1	microscopy					608:617	transmission electron microscopy	586:617	transmission electron microscopy	586:617	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite was studied by scanning electron microscopy and transmission electron microscopy and their affirmed interactions were characterised by X-ray diffraction, Raman, Fourier transform-infrared and UV-visible spectroscopy.
34694716	6	20	theme	effective	1191:1199	arg1	strategy					1201:1208	an effective strategy	1188:1208	an effective strategy for diagnosing and controlling pathogens	1188:1249	The stability of the nanocomposite coupled with antimicrobial activity enables an effective strategy for diagnosing and controlling pathogens.
34694716	4	21	theme	Candida	943:949	arg1	albicans					951:958	fungal strain Candida albicans	929:958	fungal strain Candida albicans	929:958	Interestingly, the prepared PPyNT@AGA nanocomposite exhibited 90% biofilm inhibition against gram-negative Pseudomonas aeruginosa, gram-positive Streptococcus pneumoniae and fungal strain Candida albicans with promising antimicrobial performance.
34694716	4	22	theme	AGA	789:791	arg1	nanocomposite					793:805	the prepared PPyNT@AGA nanocomposite	770:805	the prepared PPyNT@AGA nanocomposite	770:805	Interestingly, the prepared PPyNT@AGA nanocomposite exhibited 90% biofilm inhibition against gram-negative Pseudomonas aeruginosa, gram-positive Streptococcus pneumoniae and fungal strain Candida albicans with promising antimicrobial performance.
34694716	4	23	theme	biofilm	821:827	arg1	inhibition					829:838	90% biofilm inhibition	817:838	90% biofilm inhibition	817:838	Interestingly, the prepared PPyNT@AGA nanocomposite exhibited 90% biofilm inhibition against gram-negative Pseudomonas aeruginosa, gram-positive Streptococcus pneumoniae and fungal strain Candida albicans with promising antimicrobial performance.
34694716	0	24	theme	efficacy	30:37	arg1	evaluation					39:48	antimicrobial efficacy evaluation	16:48	antimicrobial efficacy evaluation	16:48	Antibiofilm and antimicrobial efficacy evaluation of polypyrrole nanotubes embedded in aminated gum acacia based nanocomposite.
34694716	4	25	theme	90	817:818	arg1	%					819:819	%	819:819	%	819:819	Interestingly, the prepared PPyNT@AGA nanocomposite exhibited 90% biofilm inhibition against gram-negative Pseudomonas aeruginosa, gram-positive Streptococcus pneumoniae and fungal strain Candida albicans with promising antimicrobial performance.
34694716	5	26	theme	AGA	1056:1058	arg1	composite					1067:1075	a PPyNT@AGA hybrid composite	1048:1075	a PPyNT@AGA hybrid composite	1048:1075	This study establishes the good inhibition of a PPyNT@AGA hybrid composite against various microorganisms.
34694716	3	27	theme	AGA	501:503	arg1	nanocomposite					524:536	a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite	484:536	a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite	484:536	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite was studied by scanning electron microscopy and transmission electron microscopy and their affirmed interactions were characterised by X-ray diffraction, Raman, Fourier transform-infrared and UV-visible spectroscopy.
34694716	0	28	theme	antimicrobial	16:28	arg1	evaluation					39:48	antimicrobial efficacy evaluation	16:48	antimicrobial efficacy evaluation	16:48	Antibiofilm and antimicrobial efficacy evaluation of polypyrrole nanotubes embedded in aminated gum acacia based nanocomposite.
34694716	5	29	theme	PPyNT	1050:1054	arg1	composite					1067:1075	a PPyNT@AGA hybrid composite	1048:1075	a PPyNT@AGA hybrid composite	1048:1075	This study establishes the good inhibition of a PPyNT@AGA hybrid composite against various microorganisms.
34694716	1	30	theme	composites	195:204	arg1	development					144:154	The sustainable development	128:154	The sustainable development of natural polysaccharide-based hybrid composites	128:204	The sustainable development of natural polysaccharide-based hybrid composites is highly important for the effective replacement of metal nanoparticles in diverse applications.
34694716	1	30	theme	composites	195:204	arg1	important					216:224	important	216:224	important	216:224	The sustainable development of natural polysaccharide-based hybrid composites is highly important for the effective replacement of metal nanoparticles in diverse applications.
34694716	1	31	theme	effective	234:242	arg1	replacement					244:254	the effective replacement	230:254	the effective replacement of metal nanoparticles in diverse applications	230:301	The sustainable development of natural polysaccharide-based hybrid composites is highly important for the effective replacement of metal nanoparticles in diverse applications.
34694716	3	32	theme	hybrid	517:522	arg1	nanocomposite					524:536	a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite	484:536	a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite	484:536	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite was studied by scanning electron microscopy and transmission electron microscopy and their affirmed interactions were characterised by X-ray diffraction, Raman, Fourier transform-infrared and UV-visible spectroscopy.
34694716	5	33	theme	hybrid	1060:1065	arg1	composite					1067:1075	a PPyNT@AGA hybrid composite	1048:1075	a PPyNT@AGA hybrid composite	1048:1075	This study establishes the good inhibition of a PPyNT@AGA hybrid composite against various microorganisms.
34694716	3	34	theme	affirmed	629:636	arg1	interactions					638:649	their affirmed interactions	623:649	their affirmed interactions	623:649	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite was studied by scanning electron microscopy and transmission electron microscopy and their affirmed interactions were characterised by X-ray diffraction, Raman, Fourier transform-infrared and UV-visible spectroscopy.
34694716	6	35	theme	antimicrobial	1157:1169	arg1	activity					1171:1178	antimicrobial activity	1157:1178	antimicrobial activity	1157:1178	The stability of the nanocomposite coupled with antimicrobial activity enables an effective strategy for diagnosing and controlling pathogens.
34694716	4	36	theme	%	819:819	arg1	inhibition					829:838	90% biofilm inhibition	817:838	90% biofilm inhibition	817:838	Interestingly, the prepared PPyNT@AGA nanocomposite exhibited 90% biofilm inhibition against gram-negative Pseudomonas aeruginosa, gram-positive Streptococcus pneumoniae and fungal strain Candida albicans with promising antimicrobial performance.
34694716	5	37	theme	good	1029:1032	arg1	inhibition					1034:1043	the good inhibition	1025:1043	the good inhibition of a PPyNT@AGA hybrid composite against various microorganisms	1025:1106	This study establishes the good inhibition of a PPyNT@AGA hybrid composite against various microorganisms.
34694716	1	38	from	replacement	244:254	arg1	applications					290:301	diverse applications	282:301	diverse applications	282:301	The sustainable development of natural polysaccharide-based hybrid composites is highly important for the effective replacement of metal nanoparticles in diverse applications.
34694716	3	39	theme	PPyNT-enhanced	486:499	arg1	AGA					512:514	PPyNT@AGA	506:514	PPyNT@AGA	506:514	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite was studied by scanning electron microscopy and transmission electron microscopy and their affirmed interactions were characterised by X-ray diffraction, Raman, Fourier transform-infrared and UV-visible spectroscopy.
34694716	3	39	theme	PPyNT-enhanced	486:499	arg1	AGA					501:503	PPyNT-enhanced AGA	486:503	a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite	484:536	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite was studied by scanning electron microscopy and transmission electron microscopy and their affirmed interactions were characterised by X-ray diffraction, Raman, Fourier transform-infrared and UV-visible spectroscopy.
34694716	0	40	from	embedded	75:82	arg1	nanocomposite					113:125	aminated gum acacia based nanocomposite	87:125	aminated gum acacia based nanocomposite	87:125	Antibiofilm and antimicrobial efficacy evaluation of polypyrrole nanotubes embedded in aminated gum acacia based nanocomposite.
34694716	5	41	theme	composite	1067:1075	arg1	inhibition					1034:1043	the good inhibition	1025:1043	the good inhibition of a PPyNT@AGA hybrid composite against various microorganisms	1025:1106	This study establishes the good inhibition of a PPyNT@AGA hybrid composite against various microorganisms.
34694716	2	42	theme	aminated	373:380	arg1	acacia					386:391	aminated gum acacia	373:391	aminated gum acacia (AGA)	373:397	Here, polypyrrole nanotubes (PPyNTs) were embedded on the surface of aminated gum acacia (AGA) to produce ecofriendly nanocomposites for biomedical applications.
34694716	2	42	theme	aminated	373:380	arg1	AGA					394:396	AGA	394:396	AGA	394:396	Here, polypyrrole nanotubes (PPyNTs) were embedded on the surface of aminated gum acacia (AGA) to produce ecofriendly nanocomposites for biomedical applications.
34694716	0	43	theme	polypyrrole	53:63	arg1	Antibiofilm					0:10	Antibiofilm	0:10	Antibiofilm	0:10	Antibiofilm and antimicrobial efficacy evaluation of polypyrrole nanotubes embedded in aminated gum acacia based nanocomposite.
34694716	0	43	theme	polypyrrole	53:63	arg1	evaluation					39:48	antimicrobial efficacy evaluation	16:48	antimicrobial efficacy evaluation	16:48	Antibiofilm and antimicrobial efficacy evaluation of polypyrrole nanotubes embedded in aminated gum acacia based nanocomposite.
34694716	3	44	theme	UV-visible	730:739	arg1	spectroscopy					741:752	Fourier transform-infrared and UV-visible spectroscopy	699:752	spectroscopy	741:752	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite was studied by scanning electron microscopy and transmission electron microscopy and their affirmed interactions were characterised by X-ray diffraction, Raman, Fourier transform-infrared and UV-visible spectroscopy.
34694716	3	44	theme	UV-visible	730:739	arg1	diffraction					679:689	X-ray diffraction	673:689	X-ray diffraction	673:689	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite was studied by scanning electron microscopy and transmission electron microscopy and their affirmed interactions were characterised by X-ray diffraction, Raman, Fourier transform-infrared and UV-visible spectroscopy.
34694716	3	45	theme	nanocomposite	524:536	arg1	morphology					470:479	The morphology	466:479	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite	466:536	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite was studied by scanning electron microscopy and transmission electron microscopy and their affirmed interactions were characterised by X-ray diffraction, Raman, Fourier transform-infrared and UV-visible spectroscopy.
34694716	1	46	theme	sustainable	132:142	arg1	development					144:154	The sustainable development	128:154	The sustainable development of natural polysaccharide-based hybrid composites	128:204	The sustainable development of natural polysaccharide-based hybrid composites is highly important for the effective replacement of metal nanoparticles in diverse applications.
34694716	1	46	theme	sustainable	132:142	arg1	important					216:224	important	216:224	important	216:224	The sustainable development of natural polysaccharide-based hybrid composites is highly important for the effective replacement of metal nanoparticles in diverse applications.
34694716	4	47	theme	@	788:788	arg1	nanocomposite					793:805	the prepared PPyNT@AGA nanocomposite	770:805	the prepared PPyNT@AGA nanocomposite	770:805	Interestingly, the prepared PPyNT@AGA nanocomposite exhibited 90% biofilm inhibition against gram-negative Pseudomonas aeruginosa, gram-positive Streptococcus pneumoniae and fungal strain Candida albicans with promising antimicrobial performance.
34694716	0	48	from	nanocomposite	113:125	arg1	embedded					75:82	embedded	75:82	embedded	75:82	Antibiofilm and antimicrobial efficacy evaluation of polypyrrole nanotubes embedded in aminated gum acacia based nanocomposite.
34694716	6	49	theme	nanocomposite	1130:1142	arg1	stability					1113:1121	The stability	1109:1121	The stability of the nanocomposite coupled with antimicrobial activity	1109:1178	The stability of the nanocomposite coupled with antimicrobial activity enables an effective strategy for diagnosing and controlling pathogens.
34694716	3	50	theme	PPyNT	506:510	arg1	AGA					512:514	PPyNT@AGA	506:514	PPyNT@AGA	506:514	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite was studied by scanning electron microscopy and transmission electron microscopy and their affirmed interactions were characterised by X-ray diffraction, Raman, Fourier transform-infrared and UV-visible spectroscopy.
34694716	3	50	theme	PPyNT	506:510	arg1	AGA					501:503	PPyNT-enhanced AGA	486:503	a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite	484:536	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite was studied by scanning electron microscopy and transmission electron microscopy and their affirmed interactions were characterised by X-ray diffraction, Raman, Fourier transform-infrared and UV-visible spectroscopy.
34694716	5	51	theme	@	1055:1055	arg1	composite					1067:1075	a PPyNT@AGA hybrid composite	1048:1075	a PPyNT@AGA hybrid composite	1048:1075	This study establishes the good inhibition of a PPyNT@AGA hybrid composite against various microorganisms.
34694716	1	52	theme	metal	259:263	arg1	nanoparticles					265:277	metal nanoparticles	259:277	metal nanoparticles	259:277	The sustainable development of natural polysaccharide-based hybrid composites is highly important for the effective replacement of metal nanoparticles in diverse applications.
34694716	3	53	theme	scanning	553:560	arg1	microscopy					571:580	scanning electron microscopy	553:580	scanning electron microscopy	553:580	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite was studied by scanning electron microscopy and transmission electron microscopy and their affirmed interactions were characterised by X-ray diffraction, Raman, Fourier transform-infrared and UV-visible spectroscopy.
34694716	3	54	theme	X-ray	673:677	arg1	Raman					692:696	Raman	692:696	Raman	692:696	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite was studied by scanning electron microscopy and transmission electron microscopy and their affirmed interactions were characterised by X-ray diffraction, Raman, Fourier transform-infrared and UV-visible spectroscopy.
34694716	3	54	theme	X-ray	673:677	arg1	transform-infrared					707:724	transform-infrared	707:724	transform-infrared	707:724	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite was studied by scanning electron microscopy and transmission electron microscopy and their affirmed interactions were characterised by X-ray diffraction, Raman, Fourier transform-infrared and UV-visible spectroscopy.
34694716	3	54	theme	X-ray	673:677	arg1	diffraction					679:689	X-ray diffraction	673:689	X-ray diffraction	673:689	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite was studied by scanning electron microscopy and transmission electron microscopy and their affirmed interactions were characterised by X-ray diffraction, Raman, Fourier transform-infrared and UV-visible spectroscopy.
34694716	3	54	theme	X-ray	673:677	arg1	spectroscopy					741:752	Fourier transform-infrared and UV-visible spectroscopy	699:752	spectroscopy	741:752	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite was studied by scanning electron microscopy and transmission electron microscopy and their affirmed interactions were characterised by X-ray diffraction, Raman, Fourier transform-infrared and UV-visible spectroscopy.
34694716	3	55	theme	@	511:511	arg1	AGA					512:514	PPyNT@AGA	506:514	PPyNT@AGA	506:514	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite was studied by scanning electron microscopy and transmission electron microscopy and their affirmed interactions were characterised by X-ray diffraction, Raman, Fourier transform-infrared and UV-visible spectroscopy.
34694716	3	55	theme	@	511:511	arg1	AGA					501:503	PPyNT-enhanced AGA	486:503	a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite	484:536	The morphology of a PPyNT-enhanced AGA (PPyNT@AGA) hybrid nanocomposite was studied by scanning electron microscopy and transmission electron microscopy and their affirmed interactions were characterised by X-ray diffraction, Raman, Fourier transform-infrared and UV-visible spectroscopy.
34694716	1	56	theme	nanoparticles	265:277	arg1	replacement					244:254	the effective replacement	230:254	the effective replacement of metal nanoparticles in diverse applications	230:301	The sustainable development of natural polysaccharide-based hybrid composites is highly important for the effective replacement of metal nanoparticles in diverse applications.
34694716	1	57	theme	natural	159:165	arg1	composites					195:204	natural polysaccharide-based hybrid composites	159:204	natural polysaccharide-based hybrid composites	159:204	The sustainable development of natural polysaccharide-based hybrid composites is highly important for the effective replacement of metal nanoparticles in diverse applications.
34694716	1	58	theme	polysaccharide-based	167:186	arg1	composites					195:204	natural polysaccharide-based hybrid composites	159:204	natural polysaccharide-based hybrid composites	159:204	The sustainable development of natural polysaccharide-based hybrid composites is highly important for the effective replacement of metal nanoparticles in diverse applications.
34694716	1	59	theme	diverse	282:288	arg1	applications					290:301	diverse applications	282:301	diverse applications	282:301	The sustainable development of natural polysaccharide-based hybrid composites is highly important for the effective replacement of metal nanoparticles in diverse applications.
34408229	0	0	theme	Chlorella	80:88	arg1	promotion					106:114	Chlorella vulgaris growth promotion	80:114	Chlorella vulgaris growth promotion	80:114	Characterization of alginate extracted from Sargassum latifolium and its use in Chlorella vulgaris growth promotion and riboflavin drug delivery.
34408229	12	1	theme	simulated	1397:1405	arg1	fluid					1415:1419	simulated gastric fluid	1397:1419	simulated gastric fluid	1397:1419	All formulations exhibited prolonged riboflavin release over 120 min in simulated gastric fluid, followed Higuchi model (R2 = 0.962-0.887) and Korsmeyer-Peppas model with Fickian release (n ranges from 0.204 to 0.3885).
34408229	11	2	theme	89.15	1276:1280	arg1	%					1281:1281	%	1281:1281	%	1281:1281	Riboflavin-entrapped barium alginate-Arabic gum polymeric matrix (beads) was formulated to achieve 89.15% optimum drug entrapment efficiency (EE%).
34408229	8	3	theme	dry	976:978	arg1	wt					980:981	620.33 mg/g dry wt	964:981	620.33 mg/g dry wt	964:981	The highest total soluble proteins and total carbohydrates were 179.22 mg/g dry wt and 620.33 mg/g dry wt, respectively.
34408229	11	4	dep	barium	1198:1203	arg1	gum					1221:1223	gum	1221:1223	gum	1221:1223	Riboflavin-entrapped barium alginate-Arabic gum polymeric matrix (beads) was formulated to achieve 89.15% optimum drug entrapment efficiency (EE%).
34408229	7	5	theme	alginate	839:846	arg1	supplementation					860:874	0.3 g/L alginate hydrolysate supplementation	831:874	0.3 g/L alginate hydrolysate supplementation	831:874	The highest stimulation (0.7 g/L biomass) was achieved by using 0.3 g/L alginate hydrolysate supplementation.
34408229	3	6	from	rich	425:428	arg1	acids					466:470	uronic acids	459:470	uronic acids (47.4%)	459:478	The chemical composition of S. latifolium is rich in total sugars (41.08%) and uronic acids (47.4%); while, proteins, lipids and sulfates contents are 4.61, 1.13 and 0.09%, respectively.
34408229	3	6	from	rich	425:428	arg1	%					477:477	47.4%	473:477	47.4%	473:477	The chemical composition of S. latifolium is rich in total sugars (41.08%) and uronic acids (47.4%); while, proteins, lipids and sulfates contents are 4.61, 1.13 and 0.09%, respectively.
34408229	3	6	from	rich	425:428	arg1	sugars					439:444	total sugars	433:444	total sugars (41.08%)	433:453	The chemical composition of S. latifolium is rich in total sugars (41.08%) and uronic acids (47.4%); while, proteins, lipids and sulfates contents are 4.61, 1.13 and 0.09%, respectively.
34408229	3	6	from	rich	425:428	arg1	%					452:452	41.08%	447:452	41.08%	447:452	The chemical composition of S. latifolium is rich in total sugars (41.08%) and uronic acids (47.4%); while, proteins, lipids and sulfates contents are 4.61, 1.13 and 0.09%, respectively.
34408229	11	7	theme	%	1281:1281	arg1	%					1321:1321	EE%	1319:1321	EE%	1319:1321	Riboflavin-entrapped barium alginate-Arabic gum polymeric matrix (beads) was formulated to achieve 89.15% optimum drug entrapment efficiency (EE%).
34408229	11	7	theme	%	1281:1281	arg1	efficiency					1307:1316	89.15% optimum drug entrapment efficiency	1276:1316	89.15% optimum drug entrapment efficiency (EE%)	1276:1322	Riboflavin-entrapped barium alginate-Arabic gum polymeric matrix (beads) was formulated to achieve 89.15% optimum drug entrapment efficiency (EE%).
34408229	8	8	theme	dry	953:955	arg1	wt					957:958	179.22 mg/g dry wt	941:958	179.22 mg/g dry wt	941:958	The highest total soluble proteins and total carbohydrates were 179.22 mg/g dry wt and 620.33 mg/g dry wt, respectively.
34408229	8	8	theme	dry	953:955	arg1	proteins					903:910	The highest total soluble proteins	877:910	The highest total soluble proteins	877:910	The highest total soluble proteins and total carbohydrates were 179.22 mg/g dry wt and 620.33 mg/g dry wt, respectively.
34408229	0	9	theme	growth	99:104	arg1	promotion					106:114	Chlorella vulgaris growth promotion	80:114	Chlorella vulgaris growth promotion	80:114	Characterization of alginate extracted from Sargassum latifolium and its use in Chlorella vulgaris growth promotion and riboflavin drug delivery.
34408229	8	10	theme	620.33 mg/g	964:974	arg1	wt					980:981	620.33 mg/g dry wt	964:981	620.33 mg/g dry wt	964:981	The highest total soluble proteins and total carbohydrates were 179.22 mg/g dry wt and 620.33 mg/g dry wt, respectively.
34408229	8	11	theme	179.22 mg/g	941:951	arg1	wt					957:958	179.22 mg/g dry wt	941:958	179.22 mg/g dry wt	941:958	The highest total soluble proteins and total carbohydrates were 179.22 mg/g dry wt and 620.33 mg/g dry wt, respectively.
34408229	8	11	theme	179.22 mg/g	941:951	arg1	proteins					903:910	The highest total soluble proteins	877:910	The highest total soluble proteins	877:910	The highest total soluble proteins and total carbohydrates were 179.22 mg/g dry wt and 620.33 mg/g dry wt, respectively.
34408229	12	12	theme	prolonged	1352:1360	arg1	release					1373:1379	prolonged riboflavin release	1352:1379	prolonged riboflavin release	1352:1379	All formulations exhibited prolonged riboflavin release over 120 min in simulated gastric fluid, followed Higuchi model (R2 = 0.962-0.887) and Korsmeyer-Peppas model with Fickian release (n ranges from 0.204 to 0.3885).
34408229	0	13	theme	vulgaris	90:97	arg1	promotion					106:114	Chlorella vulgaris growth promotion	80:114	Chlorella vulgaris growth promotion	80:114	Characterization of alginate extracted from Sargassum latifolium and its use in Chlorella vulgaris growth promotion and riboflavin drug delivery.
34408229	1	14	attach	derived	156:162	arg2	Alginates					146:154	Alginates	146:154	Alginates derived from macroalgae	146:178	Alginates derived from macroalgae have been widely used in a variety of applications due to their stability, biodegradability and biocompatibility.
34408229	1	14	attach	derived	156:162	arg1	macroalgae					169:178	macroalgae	169:178	macroalgae	169:178	Alginates derived from macroalgae have been widely used in a variety of applications due to their stability, biodegradability and biocompatibility.
34408229	2	15	dep	%	373:373	arg1	w/w					375:377	w/w	375:377	17.5% w/w	369:377	Alginate was extracted from Egyptian Sargassum latifolium thallus yielding 17.5% w/w.
34408229	9	16	theme	27.697 mg/g	1035:1045	arg1	wt					1051:1052	27.697 mg/g dry wt.	1035:1053	27.697 mg/g dry wt.	1035:1053	The highest total phenolics content (27.697 mg/g dry wt.)
34408229	9	16	theme	27.697 mg/g	1035:1045	arg1	content					1026:1032	The highest total phenolics content	998:1032	The highest total phenolics content (27.697 mg/g dry wt.)	998:1054	The highest total phenolics content (27.697 mg/g dry wt.)
34408229	10	17	dep	activity	1077:1084	arg1	guaiacol					1057:1064	, guaiacol peroxidase activity	1055:1084	guaiacol	1057:1064	, guaiacol peroxidase activity (2.899 µmol min-1 g-1) were recorded also to 0.3 g/L alginate hydrolysate supplementation.
34408229	11	18	theme	entrapment	1296:1305	arg1	%					1321:1321	EE%	1319:1321	EE%	1319:1321	Riboflavin-entrapped barium alginate-Arabic gum polymeric matrix (beads) was formulated to achieve 89.15% optimum drug entrapment efficiency (EE%).
34408229	11	18	theme	entrapment	1296:1305	arg1	efficiency					1307:1316	89.15% optimum drug entrapment efficiency	1276:1316	89.15% optimum drug entrapment efficiency (EE%)	1276:1322	Riboflavin-entrapped barium alginate-Arabic gum polymeric matrix (beads) was formulated to achieve 89.15% optimum drug entrapment efficiency (EE%).
34408229	6	19	theme	growth	750:755	arg1	promoter					757:764	Chlorella vulgaris growth promoter	731:764	Chlorella vulgaris growth promoter	731:764	Sodium alginate hydrolysate was evaluated as Chlorella vulgaris growth promoter.
34408229	6	19	theme	growth	750:755	arg1	hydrolysate					702:712	Sodium alginate hydrolysate	686:712	Sodium alginate hydrolysate	686:712	Sodium alginate hydrolysate was evaluated as Chlorella vulgaris growth promoter.
34408229	12	20	theme	gastric	1407:1413	arg1	fluid					1415:1419	simulated gastric fluid	1397:1419	simulated gastric fluid	1397:1419	All formulations exhibited prolonged riboflavin release over 120 min in simulated gastric fluid, followed Higuchi model (R2 = 0.962-0.887) and Korsmeyer-Peppas model with Fickian release (n ranges from 0.204 to 0.3885).
34408229	9	21	theme	dry	1047:1049	arg1	wt					1051:1052	27.697 mg/g dry wt.	1035:1053	27.697 mg/g dry wt.	1035:1053	The highest total phenolics content (27.697 mg/g dry wt.)
34408229	9	21	theme	dry	1047:1049	arg1	content					1026:1032	The highest total phenolics content	998:1032	The highest total phenolics content (27.697 mg/g dry wt.)	998:1054	The highest total phenolics content (27.697 mg/g dry wt.)
34408229	12	22	theme	Higuchi	1431:1437	arg1	R2 = 0.962-0.887					1446:1461	R2 = 0.962-0.887	1446:1461	R2 = 0.962-0.887	1446:1461	All formulations exhibited prolonged riboflavin release over 120 min in simulated gastric fluid, followed Higuchi model (R2 = 0.962-0.887) and Korsmeyer-Peppas model with Fickian release (n ranges from 0.204 to 0.3885).
34408229	12	22	theme	Higuchi	1431:1437	arg1	model					1439:1443	Higuchi model	1431:1443	Higuchi model (R2 = 0.962-0.887)	1431:1462	All formulations exhibited prolonged riboflavin release over 120 min in simulated gastric fluid, followed Higuchi model (R2 = 0.962-0.887) and Korsmeyer-Peppas model with Fickian release (n ranges from 0.204 to 0.3885).
34408229	3	23	from	acids	466:470	arg1	rich					425:428	rich	425:428	rich	425:428	The chemical composition of S. latifolium is rich in total sugars (41.08%) and uronic acids (47.4%); while, proteins, lipids and sulfates contents are 4.61, 1.13 and 0.09%, respectively.
34408229	3	23	from	acids	466:470	arg1	composition					393:403	The chemical composition	380:403	The chemical composition of S. latifolium	380:420	The chemical composition of S. latifolium is rich in total sugars (41.08%) and uronic acids (47.4%); while, proteins, lipids and sulfates contents are 4.61, 1.13 and 0.09%, respectively.
34408229	10	24	theme	hydrolysate	1148:1158	arg1	supplementation					1160:1174	0.3 g/L alginate hydrolysate supplementation	1131:1174	0.3 g/L alginate hydrolysate supplementation	1131:1174	, guaiacol peroxidase activity (2.899 µmol min-1 g-1) were recorded also to 0.3 g/L alginate hydrolysate supplementation.
34408229	11	25	theme	optimum	1283:1289	arg1	%					1321:1321	EE%	1319:1321	EE%	1319:1321	Riboflavin-entrapped barium alginate-Arabic gum polymeric matrix (beads) was formulated to achieve 89.15% optimum drug entrapment efficiency (EE%).
34408229	11	25	theme	optimum	1283:1289	arg1	efficiency					1307:1316	89.15% optimum drug entrapment efficiency	1276:1316	89.15% optimum drug entrapment efficiency (EE%)	1276:1322	Riboflavin-entrapped barium alginate-Arabic gum polymeric matrix (beads) was formulated to achieve 89.15% optimum drug entrapment efficiency (EE%).
34408229	6	26	theme	Chlorella	731:739	arg1	promoter					757:764	Chlorella vulgaris growth promoter	731:764	Chlorella vulgaris growth promoter	731:764	Sodium alginate hydrolysate was evaluated as Chlorella vulgaris growth promoter.
34408229	6	26	theme	Chlorella	731:739	arg1	hydrolysate					702:712	Sodium alginate hydrolysate	686:712	Sodium alginate hydrolysate	686:712	Sodium alginate hydrolysate was evaluated as Chlorella vulgaris growth promoter.
34408229	12	27	theme	riboflavin	1362:1371	arg1	release					1373:1379	prolonged riboflavin release	1352:1379	prolonged riboflavin release	1352:1379	All formulations exhibited prolonged riboflavin release over 120 min in simulated gastric fluid, followed Higuchi model (R2 = 0.962-0.887) and Korsmeyer-Peppas model with Fickian release (n ranges from 0.204 to 0.3885).
34408229	12	28	from	120 min	1386:1392	arg1	fluid					1415:1419	simulated gastric fluid	1397:1419	simulated gastric fluid	1397:1419	All formulations exhibited prolonged riboflavin release over 120 min in simulated gastric fluid, followed Higuchi model (R2 = 0.962-0.887) and Korsmeyer-Peppas model with Fickian release (n ranges from 0.204 to 0.3885).
34408229	3	29	theme	total	433:437	arg1	sugars					439:444	total sugars	433:444	total sugars (41.08%)	433:453	The chemical composition of S. latifolium is rich in total sugars (41.08%) and uronic acids (47.4%); while, proteins, lipids and sulfates contents are 4.61, 1.13 and 0.09%, respectively.
34408229	3	29	theme	total	433:437	arg1	%					452:452	41.08%	447:452	41.08%	447:452	The chemical composition of S. latifolium is rich in total sugars (41.08%) and uronic acids (47.4%); while, proteins, lipids and sulfates contents are 4.61, 1.13 and 0.09%, respectively.
34408229	11	30	theme	drug	1291:1294	arg1	%					1321:1321	EE%	1319:1321	EE%	1319:1321	Riboflavin-entrapped barium alginate-Arabic gum polymeric matrix (beads) was formulated to achieve 89.15% optimum drug entrapment efficiency (EE%).
34408229	11	30	theme	drug	1291:1294	arg1	efficiency					1307:1316	89.15% optimum drug entrapment efficiency	1276:1316	89.15% optimum drug entrapment efficiency (EE%)	1276:1322	Riboflavin-entrapped barium alginate-Arabic gum polymeric matrix (beads) was formulated to achieve 89.15% optimum drug entrapment efficiency (EE%).
34408229	2	31	theme	Egyptian	322:329	arg1	Sargassum					331:339	Egyptian Sargassum	322:339	Egyptian Sargassum	322:339	Alginate was extracted from Egyptian Sargassum latifolium thallus yielding 17.5% w/w.
34408229	3	32	theme	chemical	384:391	arg1	rich					425:428	rich	425:428	rich	425:428	The chemical composition of S. latifolium is rich in total sugars (41.08%) and uronic acids (47.4%); while, proteins, lipids and sulfates contents are 4.61, 1.13 and 0.09%, respectively.
34408229	3	32	theme	chemical	384:391	arg1	composition					393:403	The chemical composition	380:403	The chemical composition of S. latifolium	380:420	The chemical composition of S. latifolium is rich in total sugars (41.08%) and uronic acids (47.4%); while, proteins, lipids and sulfates contents are 4.61, 1.13 and 0.09%, respectively.
34408229	11	33	theme	EE	1319:1320	arg1	%					1321:1321	EE%	1319:1321	EE%	1319:1321	Riboflavin-entrapped barium alginate-Arabic gum polymeric matrix (beads) was formulated to achieve 89.15% optimum drug entrapment efficiency (EE%).
34408229	11	33	theme	EE	1319:1320	arg1	efficiency					1307:1316	89.15% optimum drug entrapment efficiency	1276:1316	89.15% optimum drug entrapment efficiency (EE%)	1276:1322	Riboflavin-entrapped barium alginate-Arabic gum polymeric matrix (beads) was formulated to achieve 89.15% optimum drug entrapment efficiency (EE%).
34408229	0	34	theme	drug	131:134	arg1	delivery					136:143	riboflavin drug delivery	120:143	riboflavin drug delivery	120:143	Characterization of alginate extracted from Sargassum latifolium and its use in Chlorella vulgaris growth promotion and riboflavin drug delivery.
34408229	7	35	theme	0.7 g/L	792:798	arg1	stimulation					779:789	The highest stimulation	767:789	The highest stimulation (0.7 g/L biomass)	767:807	The highest stimulation (0.7 g/L biomass) was achieved by using 0.3 g/L alginate hydrolysate supplementation.
34408229	7	35	theme	0.7 g/L	792:798	arg1	biomass					800:806	0.7 g/L biomass	792:806	0.7 g/L biomass	792:806	The highest stimulation (0.7 g/L biomass) was achieved by using 0.3 g/L alginate hydrolysate supplementation.
34408229	0	36	theme	alginate	20:27	arg1	Characterization					0:15	Characterization	0:15	Characterization of alginate	0:27	Characterization of alginate extracted from Sargassum latifolium and its use in Chlorella vulgaris growth promotion and riboflavin drug delivery.
34408229	0	37	theme	riboflavin	120:129	arg1	delivery					136:143	riboflavin drug delivery	120:143	riboflavin drug delivery	120:143	Characterization of alginate extracted from Sargassum latifolium and its use in Chlorella vulgaris growth promotion and riboflavin drug delivery.
34408229	11	38	theme	Riboflavin-entrapped	1177:1196	arg1	barium					1198:1203	Riboflavin-entrapped barium	1177:1203	Riboflavin-entrapped barium alginate-Arabic gum polymeric matrix (beads)	1177:1248	Riboflavin-entrapped barium alginate-Arabic gum polymeric matrix (beads) was formulated to achieve 89.15% optimum drug entrapment efficiency (EE%).
34408229	7	39	theme	highest	771:777	arg1	stimulation					779:789	The highest stimulation	767:789	The highest stimulation (0.7 g/L biomass)	767:807	The highest stimulation (0.7 g/L biomass) was achieved by using 0.3 g/L alginate hydrolysate supplementation.
34408229	7	39	theme	highest	771:777	arg1	biomass					800:806	0.7 g/L biomass	792:806	0.7 g/L biomass	792:806	The highest stimulation (0.7 g/L biomass) was achieved by using 0.3 g/L alginate hydrolysate supplementation.
34408229	3	40	theme	uronic	459:464	arg1	acids					466:470	uronic acids	459:470	uronic acids (47.4%)	459:478	The chemical composition of S. latifolium is rich in total sugars (41.08%) and uronic acids (47.4%); while, proteins, lipids and sulfates contents are 4.61, 1.13 and 0.09%, respectively.
34408229	3	40	theme	uronic	459:464	arg1	%					477:477	47.4%	473:477	47.4%	473:477	The chemical composition of S. latifolium is rich in total sugars (41.08%) and uronic acids (47.4%); while, proteins, lipids and sulfates contents are 4.61, 1.13 and 0.09%, respectively.
34408229	12	41	theme	Fickian	1496:1502	arg1	release					1504:1510	Fickian release	1496:1510	Fickian release	1496:1510	All formulations exhibited prolonged riboflavin release over 120 min in simulated gastric fluid, followed Higuchi model (R2 = 0.962-0.887) and Korsmeyer-Peppas model with Fickian release (n ranges from 0.204 to 0.3885).
34408229	9	42	theme	total	1010:1014	arg1	wt					1051:1052	27.697 mg/g dry wt.	1035:1053	27.697 mg/g dry wt.	1035:1053	The highest total phenolics content (27.697 mg/g dry wt.)
34408229	9	42	theme	total	1010:1014	arg1	content					1026:1032	The highest total phenolics content	998:1032	The highest total phenolics content (27.697 mg/g dry wt.)	998:1054	The highest total phenolics content (27.697 mg/g dry wt.)
34408229	10	43	theme	peroxidase	1066:1075	arg1	2.899 µmol min-1 g-1					1087:1106	2.899 µmol min-1 g-1	1087:1106	2.899 µmol min-1 g-1	1087:1106	, guaiacol peroxidase activity (2.899 µmol min-1 g-1) were recorded also to 0.3 g/L alginate hydrolysate supplementation.
34408229	10	43	theme	peroxidase	1066:1075	arg1	activity					1077:1084	, guaiacol peroxidase activity	1055:1084	activity	1077:1084	, guaiacol peroxidase activity (2.899 µmol min-1 g-1) were recorded also to 0.3 g/L alginate hydrolysate supplementation.
34408229	11	44	theme	polymeric	1225:1233	arg1	beads					1243:1247	polymeric matrix (beads)	1225:1248	polymeric matrix (beads)	1225:1248	Riboflavin-entrapped barium alginate-Arabic gum polymeric matrix (beads) was formulated to achieve 89.15% optimum drug entrapment efficiency (EE%).
34408229	0	45	from	use	73:75	arg1	delivery					136:143	riboflavin drug delivery	120:143	riboflavin drug delivery	120:143	Characterization of alginate extracted from Sargassum latifolium and its use in Chlorella vulgaris growth promotion and riboflavin drug delivery.
34408229	0	45	from	use	73:75	arg1	promotion					106:114	Chlorella vulgaris growth promotion	80:114	Chlorella vulgaris growth promotion	80:114	Characterization of alginate extracted from Sargassum latifolium and its use in Chlorella vulgaris growth promotion and riboflavin drug delivery.
34408229	10	46	theme	alginate	1139:1146	arg1	supplementation					1160:1174	0.3 g/L alginate hydrolysate supplementation	1131:1174	0.3 g/L alginate hydrolysate supplementation	1131:1174	, guaiacol peroxidase activity (2.899 µmol min-1 g-1) were recorded also to 0.3 g/L alginate hydrolysate supplementation.
34408229	8	47	theme	total	889:893	arg1	wt					957:958	179.22 mg/g dry wt	941:958	179.22 mg/g dry wt	941:958	The highest total soluble proteins and total carbohydrates were 179.22 mg/g dry wt and 620.33 mg/g dry wt, respectively.
34408229	8	47	theme	total	889:893	arg1	proteins					903:910	The highest total soluble proteins	877:910	The highest total soluble proteins	877:910	The highest total soluble proteins and total carbohydrates were 179.22 mg/g dry wt and 620.33 mg/g dry wt, respectively.
34408229	3	48	theme	latifolium	411:420	arg1	rich					425:428	rich	425:428	rich	425:428	The chemical composition of S. latifolium is rich in total sugars (41.08%) and uronic acids (47.4%); while, proteins, lipids and sulfates contents are 4.61, 1.13 and 0.09%, respectively.
34408229	3	48	theme	latifolium	411:420	arg1	composition					393:403	The chemical composition	380:403	The chemical composition of S. latifolium	380:420	The chemical composition of S. latifolium is rich in total sugars (41.08%) and uronic acids (47.4%); while, proteins, lipids and sulfates contents are 4.61, 1.13 and 0.09%, respectively.
34408229	5	49	theme	alginate	653:660	arg1	nature					678:683	alginate semicrystalline nature	653:683	alginate semicrystalline nature	653:683	Crystallinity index (0.334) indicates alginate semicrystalline nature.
34408229	8	50	theme	highest	881:887	arg1	wt					957:958	179.22 mg/g dry wt	941:958	179.22 mg/g dry wt	941:958	The highest total soluble proteins and total carbohydrates were 179.22 mg/g dry wt and 620.33 mg/g dry wt, respectively.
34408229	8	50	theme	highest	881:887	arg1	proteins					903:910	The highest total soluble proteins	877:910	The highest total soluble proteins	877:910	The highest total soluble proteins and total carbohydrates were 179.22 mg/g dry wt and 620.33 mg/g dry wt, respectively.
34408229	3	51	dep	proteins	488:495	arg1	contents					518:525	contents	518:525	contents	518:525	The chemical composition of S. latifolium is rich in total sugars (41.08%) and uronic acids (47.4%); while, proteins, lipids and sulfates contents are 4.61, 1.13 and 0.09%, respectively.
34408229	11	52	theme	matrix	1235:1240	arg1	beads					1243:1247	polymeric matrix (beads)	1225:1248	polymeric matrix (beads)	1225:1248	Riboflavin-entrapped barium alginate-Arabic gum polymeric matrix (beads) was formulated to achieve 89.15% optimum drug entrapment efficiency (EE%).
34408229	12	53	theme	Korsmeyer-Peppas	1468:1483	arg1	model					1485:1489	Korsmeyer-Peppas model	1468:1489	Korsmeyer-Peppas model	1468:1489	All formulations exhibited prolonged riboflavin release over 120 min in simulated gastric fluid, followed Higuchi model (R2 = 0.962-0.887) and Korsmeyer-Peppas model with Fickian release (n ranges from 0.204 to 0.3885).
34408229	4	54	dep	NMR	567:569	arg1	analyses					585:592	analyses	585:592	analyses	585:592	NMR, FTIR and TGA analyses were also performed.
34408229	6	55	theme	alginate	693:700	arg1	promoter					757:764	Chlorella vulgaris growth promoter	731:764	Chlorella vulgaris growth promoter	731:764	Sodium alginate hydrolysate was evaluated as Chlorella vulgaris growth promoter.
34408229	6	55	theme	alginate	693:700	arg1	hydrolysate					702:712	Sodium alginate hydrolysate	686:712	Sodium alginate hydrolysate	686:712	Sodium alginate hydrolysate was evaluated as Chlorella vulgaris growth promoter.
34408229	5	56	theme	Crystallinity	615:627	arg1	0.334					636:640	0.334	636:640	0.334	636:640	Crystallinity index (0.334) indicates alginate semicrystalline nature.
34408229	5	56	theme	Crystallinity	615:627	arg1	index					629:633	Crystallinity index	615:633	Crystallinity index (0.334)	615:641	Crystallinity index (0.334) indicates alginate semicrystalline nature.
34408229	6	57	theme	vulgaris	741:748	arg1	promoter					757:764	Chlorella vulgaris growth promoter	731:764	Chlorella vulgaris growth promoter	731:764	Sodium alginate hydrolysate was evaluated as Chlorella vulgaris growth promoter.
34408229	6	57	theme	vulgaris	741:748	arg1	hydrolysate					702:712	Sodium alginate hydrolysate	686:712	Sodium alginate hydrolysate	686:712	Sodium alginate hydrolysate was evaluated as Chlorella vulgaris growth promoter.
34408229	1	58	used	used	197:200	arg2	Alginates					146:154	Alginates	146:154	Alginates derived from macroalgae	146:178	Alginates derived from macroalgae have been widely used in a variety of applications due to their stability, biodegradability and biocompatibility.
34408229	6	59	theme	Sodium	686:691	arg1	promoter					757:764	Chlorella vulgaris growth promoter	731:764	Chlorella vulgaris growth promoter	731:764	Sodium alginate hydrolysate was evaluated as Chlorella vulgaris growth promoter.
34408229	6	59	theme	Sodium	686:691	arg1	hydrolysate					702:712	Sodium alginate hydrolysate	686:712	Sodium alginate hydrolysate	686:712	Sodium alginate hydrolysate was evaluated as Chlorella vulgaris growth promoter.
34408229	1	60	theme	applications	218:229	arg1	applications					218:229	applications	218:229	applications due to their stability, biodegradability and biocompatibility	218:291	Alginates derived from macroalgae have been widely used in a variety of applications due to their stability, biodegradability and biocompatibility.
34408229	1	60	theme	applications	218:229	arg1	variety					207:213	a variety	205:213	a variety of applications due to their stability, biodegradability and biocompatibility	205:291	Alginates derived from macroalgae have been widely used in a variety of applications due to their stability, biodegradability and biocompatibility.
34408229	8	61	theme	soluble	895:901	arg1	wt					957:958	179.22 mg/g dry wt	941:958	179.22 mg/g dry wt	941:958	The highest total soluble proteins and total carbohydrates were 179.22 mg/g dry wt and 620.33 mg/g dry wt, respectively.
34408229	8	61	theme	soluble	895:901	arg1	proteins					903:910	The highest total soluble proteins	877:910	The highest total soluble proteins	877:910	The highest total soluble proteins and total carbohydrates were 179.22 mg/g dry wt and 620.33 mg/g dry wt, respectively.
34408229	8	62	theme	total	916:920	arg1	carbohydrates					922:934	total carbohydrates	916:934	total carbohydrates	916:934	The highest total soluble proteins and total carbohydrates were 179.22 mg/g dry wt and 620.33 mg/g dry wt, respectively.
34408229	7	63	theme	hydrolysate	848:858	arg1	supplementation					860:874	0.3 g/L alginate hydrolysate supplementation	831:874	0.3 g/L alginate hydrolysate supplementation	831:874	The highest stimulation (0.7 g/L biomass) was achieved by using 0.3 g/L alginate hydrolysate supplementation.
34408229	1	64	theme	due	231:233	arg1	applications					218:229	applications	218:229	applications due to their stability, biodegradability and biocompatibility	218:291	Alginates derived from macroalgae have been widely used in a variety of applications due to their stability, biodegradability and biocompatibility.
34408229	11	65	dep	gum	1221:1223	arg1	alginate-Arabic					1205:1219	alginate-Arabic	1205:1219	alginate-Arabic	1205:1219	Riboflavin-entrapped barium alginate-Arabic gum polymeric matrix (beads) was formulated to achieve 89.15% optimum drug entrapment efficiency (EE%).
34408229	12	66	dep	0.3885	1536:1541	arg1	to					1533:1534	to	1533:1534	to	1533:1534	All formulations exhibited prolonged riboflavin release over 120 min in simulated gastric fluid, followed Higuchi model (R2 = 0.962-0.887) and Korsmeyer-Peppas model with Fickian release (n ranges from 0.204 to 0.3885).
34408229	3	67	from	sugars	439:444	arg1	rich					425:428	rich	425:428	rich	425:428	The chemical composition of S. latifolium is rich in total sugars (41.08%) and uronic acids (47.4%); while, proteins, lipids and sulfates contents are 4.61, 1.13 and 0.09%, respectively.
34408229	3	67	from	sugars	439:444	arg1	composition					393:403	The chemical composition	380:403	The chemical composition of S. latifolium	380:420	The chemical composition of S. latifolium is rich in total sugars (41.08%) and uronic acids (47.4%); while, proteins, lipids and sulfates contents are 4.61, 1.13 and 0.09%, respectively.
34408229	9	68	theme	highest	1002:1008	arg1	wt					1051:1052	27.697 mg/g dry wt.	1035:1053	27.697 mg/g dry wt.	1035:1053	The highest total phenolics content (27.697 mg/g dry wt.)
34408229	9	68	theme	highest	1002:1008	arg1	content					1026:1032	The highest total phenolics content	998:1032	The highest total phenolics content (27.697 mg/g dry wt.)	998:1054	The highest total phenolics content (27.697 mg/g dry wt.)
34408229	9	69	theme	phenolics	1016:1024	arg1	wt					1051:1052	27.697 mg/g dry wt.	1035:1053	27.697 mg/g dry wt.	1035:1053	The highest total phenolics content (27.697 mg/g dry wt.)
34408229	9	69	theme	phenolics	1016:1024	arg1	content					1026:1032	The highest total phenolics content	998:1032	The highest total phenolics content (27.697 mg/g dry wt.)	998:1054	The highest total phenolics content (27.697 mg/g dry wt.)
34408229	10	70	theme	0.3 g/L	1131:1137	arg1	supplementation					1160:1174	0.3 g/L alginate hydrolysate supplementation	1131:1174	0.3 g/L alginate hydrolysate supplementation	1131:1174	, guaiacol peroxidase activity (2.899 µmol min-1 g-1) were recorded also to 0.3 g/L alginate hydrolysate supplementation.
34408229	7	71	theme	0.3 g/L	831:837	arg1	supplementation					860:874	0.3 g/L alginate hydrolysate supplementation	831:874	0.3 g/L alginate hydrolysate supplementation	831:874	The highest stimulation (0.7 g/L biomass) was achieved by using 0.3 g/L alginate hydrolysate supplementation.
34408229	5	72	theme	semicrystalline	662:676	arg1	nature					678:683	alginate semicrystalline nature	653:683	alginate semicrystalline nature	653:683	Crystallinity index (0.334) indicates alginate semicrystalline nature.
33142583	7	0	theme	osteogenic	929:938	arg1	ability					956:962	the osteogenic differentiation ability	925:962	the osteogenic differentiation ability	925:962	The alkaline phosphatase (ALP) and alizarin red (ARS) staining results suggested that the osteogenic differentiation ability was improved in rCL/CS composite scaffold.
33142583	4	1	theme	nanofibers	549:558	arg1	matrix					571:576	rCL nanofibers imbibed CS matrix	545:576	rCL nanofibers imbibed CS matrix	545:576	As-prepared rCL/CS composite scaffold showed unique porous morphology with rCL nanofibers imbibed CS matrix.
33142583	8	2	theme	fabrication	1024:1034	arg1	idea					1036:1039	the novel fabrication idea	1014:1039	the novel fabrication idea	1014:1039	Hence, the novel fabrication idea and the obtained results suggested that the rCL/CS composite hydrogel scaffolds could be a promising three-dimensional bio-scaffold for bone tissue engineering.
33142583	1	3	theme	major	192:196	arg1	challenge					198:206	a major challenge	190:206	a major challenge in bone tissue engineering	190:233	Natural hydrogel scaffolds usually exhibit insufficient mechanical strength which remains a major challenge in bone tissue engineering.
33142583	3	4	theme	rCL	369:371	arg1	nanofibers					373:382	The rCL nanofibers	365:382	The rCL nanofibers	365:382	The rCL nanofibers were regenerated from deacetylation of electrospun cellulose acetate (CA) nanofibers.
33142583	4	5	theme	rCL	545:547	arg1	matrix					571:576	rCL nanofibers imbibed CS matrix	545:576	rCL nanofibers imbibed CS matrix	545:576	As-prepared rCL/CS composite scaffold showed unique porous morphology with rCL nanofibers imbibed CS matrix.
33142583	8	6	theme	composite	1092:1100	arg1	scaffolds					1111:1119	the rCL/CS composite hydrogel scaffolds	1081:1119	the rCL/CS composite hydrogel scaffolds	1081:1119	Hence, the novel fabrication idea and the obtained results suggested that the rCL/CS composite hydrogel scaffolds could be a promising three-dimensional bio-scaffold for bone tissue engineering.
33142583	8	6	theme	composite	1092:1100	arg1	bio-scaffold					1160:1171	a promising three-dimensional bio-scaffold	1130:1171	a promising three-dimensional bio-scaffold for bone tissue engineering	1130:1199	Hence, the novel fabrication idea and the obtained results suggested that the rCL/CS composite hydrogel scaffolds could be a promising three-dimensional bio-scaffold for bone tissue engineering.
33142583	8	7	theme	novel	1018:1022	arg1	idea					1036:1039	the novel fabrication idea	1014:1039	the novel fabrication idea	1014:1039	Hence, the novel fabrication idea and the obtained results suggested that the rCL/CS composite hydrogel scaffolds could be a promising three-dimensional bio-scaffold for bone tissue engineering.
33142583	2	8	theme	rCL	320:322	arg1	nanofibers					325:334	regenerated cellulose (rCL) nanofibers	297:334	regenerated cellulose (rCL) nanofibers	297:334	In this study, the limitation was addressed by incorporating regenerated cellulose (rCL) nanofibers into chitosan (CS) hydrogel.
33142583	2	9	theme	chitosan	341:348	arg1	hydrogel					355:362	chitosan (CS) hydrogel	341:362	chitosan (CS) hydrogel	341:362	In this study, the limitation was addressed by incorporating regenerated cellulose (rCL) nanofibers into chitosan (CS) hydrogel.
33142583	8	10	theme	rCL/CS	1085:1090	arg1	scaffolds					1111:1119	the rCL/CS composite hydrogel scaffolds	1081:1119	the rCL/CS composite hydrogel scaffolds	1081:1119	Hence, the novel fabrication idea and the obtained results suggested that the rCL/CS composite hydrogel scaffolds could be a promising three-dimensional bio-scaffold for bone tissue engineering.
33142583	8	10	theme	rCL/CS	1085:1090	arg1	bio-scaffold					1160:1171	a promising three-dimensional bio-scaffold	1130:1171	a promising three-dimensional bio-scaffold for bone tissue engineering	1130:1199	Hence, the novel fabrication idea and the obtained results suggested that the rCL/CS composite hydrogel scaffolds could be a promising three-dimensional bio-scaffold for bone tissue engineering.
33142583	5	11	theme	higher	649:654	arg1	strength					668:675	higher compressive strength	649:675	higher compressive strength	649:675	The compressive strength test exhibited that the rCL/CS scaffold have higher compressive strength compared to pure CS.
33142583	7	12	theme	staining	893:900	arg1	results					902:908	The alkaline phosphatase (ALP) and alizarin red (ARS) staining results	839:908	The alkaline phosphatase (ALP) and alizarin red (ARS) staining results	839:908	The alkaline phosphatase (ALP) and alizarin red (ARS) staining results suggested that the osteogenic differentiation ability was improved in rCL/CS composite scaffold.
33142583	5	13	theme	compressive	656:666	arg1	strength					668:675	higher compressive strength	649:675	higher compressive strength	649:675	The compressive strength test exhibited that the rCL/CS scaffold have higher compressive strength compared to pure CS.
33142583	2	14	theme	cellulose	309:317	arg1	nanofibers					325:334	regenerated cellulose (rCL) nanofibers	297:334	regenerated cellulose (rCL) nanofibers	297:334	In this study, the limitation was addressed by incorporating regenerated cellulose (rCL) nanofibers into chitosan (CS) hydrogel.
33142583	5	15	contain	have	644:647	arg2	strength					668:675	higher compressive strength	649:675	higher compressive strength	649:675	The compressive strength test exhibited that the rCL/CS scaffold have higher compressive strength compared to pure CS.
33142583	5	15	contain	have	644:647	arg1	scaffold					635:642	the rCL/CS scaffold	624:642	the rCL/CS scaffold	624:642	The compressive strength test exhibited that the rCL/CS scaffold have higher compressive strength compared to pure CS.
33142583	4	16	theme	porous	522:527	arg1	morphology					529:538	unique porous morphology	515:538	unique porous morphology with rCL nanofibers imbibed CS matrix	515:576	As-prepared rCL/CS composite scaffold showed unique porous morphology with rCL nanofibers imbibed CS matrix.
33142583	0	17	theme	hydrogel	52:59	arg1	scaffolds					61:69	chitosan hydrogel scaffolds	43:69	chitosan hydrogel scaffolds for bone tissue engineering	43:97	Regenerated cellulose nanofiber reinforced chitosan hydrogel scaffolds for bone tissue engineering.
33142583	7	18	theme	composite	987:995	arg1	scaffold					997:1004	rCL/CS composite scaffold	980:1004	rCL/CS composite scaffold	980:1004	The alkaline phosphatase (ALP) and alizarin red (ARS) staining results suggested that the osteogenic differentiation ability was improved in rCL/CS composite scaffold.
33142583	2	19	theme	regenerated	297:307	arg1	nanofibers					325:334	regenerated cellulose (rCL) nanofibers	297:334	regenerated cellulose (rCL) nanofibers	297:334	In this study, the limitation was addressed by incorporating regenerated cellulose (rCL) nanofibers into chitosan (CS) hydrogel.
33142583	6	20	theme	enhanced	757:764	arg1	MC3T3-E1					787:794	MC3T3-E1	787:794	MC3T3-E1	787:794	The rCL/CS scaffold showed increased biomineralization and enhanced pre-osteoblast cell (MC3T3-E1) viability, attachment, and proliferation.
33142583	6	20	theme	enhanced	757:764	arg1	cell					781:784	enhanced pre-osteoblast cell	757:784	enhanced pre-osteoblast cell	757:784	The rCL/CS scaffold showed increased biomineralization and enhanced pre-osteoblast cell (MC3T3-E1) viability, attachment, and proliferation.
33142583	3	21	theme	nanofibers	458:467	arg1	deacetylation					406:418	deacetylation	406:418	deacetylation of electrospun cellulose acetate (CA) nanofibers	406:467	The rCL nanofibers were regenerated from deacetylation of electrospun cellulose acetate (CA) nanofibers.
33142583	0	22	theme	cellulose	12:20	arg1	nanofiber					22:30	Regenerated cellulose nanofiber	0:30	Regenerated cellulose nanofiber	0:30	Regenerated cellulose nanofiber reinforced chitosan hydrogel scaffolds for bone tissue engineering.
33142583	4	23	theme	unique	515:520	arg1	morphology					529:538	unique porous morphology	515:538	unique porous morphology with rCL nanofibers imbibed CS matrix	515:576	As-prepared rCL/CS composite scaffold showed unique porous morphology with rCL nanofibers imbibed CS matrix.
33142583	1	24	theme	Natural	100:106	arg1	scaffolds					117:125	Natural hydrogel scaffolds	100:125	Natural hydrogel scaffolds	100:125	Natural hydrogel scaffolds usually exhibit insufficient mechanical strength which remains a major challenge in bone tissue engineering.
33142583	1	25	theme	bone	211:214	arg1	engineering					223:233	bone tissue engineering	211:233	bone tissue engineering	211:233	Natural hydrogel scaffolds usually exhibit insufficient mechanical strength which remains a major challenge in bone tissue engineering.
33142583	0	26	theme	Regenerated	0:10	arg1	nanofiber					22:30	Regenerated cellulose nanofiber	0:30	Regenerated cellulose nanofiber	0:30	Regenerated cellulose nanofiber reinforced chitosan hydrogel scaffolds for bone tissue engineering.
33142583	5	27	theme	pure	689:692	arg1	CS					694:695	pure CS	689:695	pure CS	689:695	The compressive strength test exhibited that the rCL/CS scaffold have higher compressive strength compared to pure CS.
33142583	1	28	theme	hydrogel	108:115	arg1	scaffolds					117:125	Natural hydrogel scaffolds	100:125	Natural hydrogel scaffolds	100:125	Natural hydrogel scaffolds usually exhibit insufficient mechanical strength which remains a major challenge in bone tissue engineering.
33142583	7	29	theme	red	883:885	arg1	staining					893:900	alizarin red (ARS) staining	874:900	alizarin red (ARS) staining	874:900	The alkaline phosphatase (ALP) and alizarin red (ARS) staining results suggested that the osteogenic differentiation ability was improved in rCL/CS composite scaffold.
33142583	1	30	theme	tissue	216:221	arg1	engineering					223:233	bone tissue engineering	211:233	bone tissue engineering	211:233	Natural hydrogel scaffolds usually exhibit insufficient mechanical strength which remains a major challenge in bone tissue engineering.
33142583	8	31	theme	promising	1132:1140	arg1	scaffolds					1111:1119	the rCL/CS composite hydrogel scaffolds	1081:1119	the rCL/CS composite hydrogel scaffolds	1081:1119	Hence, the novel fabrication idea and the obtained results suggested that the rCL/CS composite hydrogel scaffolds could be a promising three-dimensional bio-scaffold for bone tissue engineering.
33142583	8	31	theme	promising	1132:1140	arg1	bio-scaffold					1160:1171	a promising three-dimensional bio-scaffold	1130:1171	a promising three-dimensional bio-scaffold for bone tissue engineering	1130:1199	Hence, the novel fabrication idea and the obtained results suggested that the rCL/CS composite hydrogel scaffolds could be a promising three-dimensional bio-scaffold for bone tissue engineering.
33142583	5	32	theme	rCL/CS	628:633	arg1	scaffold					635:642	the rCL/CS scaffold	624:642	the rCL/CS scaffold	624:642	The compressive strength test exhibited that the rCL/CS scaffold have higher compressive strength compared to pure CS.
33142583	6	33	theme	increased	725:733	arg1	biomineralization					735:751	increased biomineralization	725:751	increased biomineralization	725:751	The rCL/CS scaffold showed increased biomineralization and enhanced pre-osteoblast cell (MC3T3-E1) viability, attachment, and proliferation.
33142583	7	34	theme	phosphatase	852:862	arg1	results					902:908	The alkaline phosphatase (ALP) and alizarin red (ARS) staining results	839:908	The alkaline phosphatase (ALP) and alizarin red (ARS) staining results	839:908	The alkaline phosphatase (ALP) and alizarin red (ARS) staining results suggested that the osteogenic differentiation ability was improved in rCL/CS composite scaffold.
33142583	4	35	theme	As-prepared	470:480	arg1	scaffold					499:506	As-prepared rCL/CS composite scaffold	470:506	As-prepared rCL/CS composite scaffold	470:506	As-prepared rCL/CS composite scaffold showed unique porous morphology with rCL nanofibers imbibed CS matrix.
33142583	7	36	theme	alizarin	874:881	arg1	staining					893:900	alizarin red (ARS) staining	874:900	alizarin red (ARS) staining	874:900	The alkaline phosphatase (ALP) and alizarin red (ARS) staining results suggested that the osteogenic differentiation ability was improved in rCL/CS composite scaffold.
33142583	3	37	theme	cellulose	435:443	arg1	nanofibers					458:467	electrospun cellulose acetate (CA) nanofibers	423:467	electrospun cellulose acetate (CA) nanofibers	423:467	The rCL nanofibers were regenerated from deacetylation of electrospun cellulose acetate (CA) nanofibers.
33142583	6	38	theme	pre-osteoblast	766:779	arg1	MC3T3-E1					787:794	MC3T3-E1	787:794	MC3T3-E1	787:794	The rCL/CS scaffold showed increased biomineralization and enhanced pre-osteoblast cell (MC3T3-E1) viability, attachment, and proliferation.
33142583	6	38	theme	pre-osteoblast	766:779	arg1	cell					781:784	enhanced pre-osteoblast cell	757:784	enhanced pre-osteoblast cell	757:784	The rCL/CS scaffold showed increased biomineralization and enhanced pre-osteoblast cell (MC3T3-E1) viability, attachment, and proliferation.
33142583	3	39	theme	CA	454:455	arg1	nanofibers					458:467	electrospun cellulose acetate (CA) nanofibers	423:467	electrospun cellulose acetate (CA) nanofibers	423:467	The rCL nanofibers were regenerated from deacetylation of electrospun cellulose acetate (CA) nanofibers.
33142583	0	40	theme	chitosan	43:50	arg1	scaffolds					61:69	chitosan hydrogel scaffolds	43:69	chitosan hydrogel scaffolds for bone tissue engineering	43:97	Regenerated cellulose nanofiber reinforced chitosan hydrogel scaffolds for bone tissue engineering.
33142583	4	41	theme	CS	568:569	arg1	matrix					571:576	rCL nanofibers imbibed CS matrix	545:576	rCL nanofibers imbibed CS matrix	545:576	As-prepared rCL/CS composite scaffold showed unique porous morphology with rCL nanofibers imbibed CS matrix.
33142583	3	42	theme	acetate	445:451	arg1	nanofibers					458:467	electrospun cellulose acetate (CA) nanofibers	423:467	electrospun cellulose acetate (CA) nanofibers	423:467	The rCL nanofibers were regenerated from deacetylation of electrospun cellulose acetate (CA) nanofibers.
33142583	4	43	theme	composite	489:497	arg1	scaffold					499:506	As-prepared rCL/CS composite scaffold	470:506	As-prepared rCL/CS composite scaffold	470:506	As-prepared rCL/CS composite scaffold showed unique porous morphology with rCL nanofibers imbibed CS matrix.
33142583	4	44	theme	imbibed	560:566	arg1	matrix					571:576	rCL nanofibers imbibed CS matrix	545:576	rCL nanofibers imbibed CS matrix	545:576	As-prepared rCL/CS composite scaffold showed unique porous morphology with rCL nanofibers imbibed CS matrix.
33142583	8	45	theme	bone	1177:1180	arg1	engineering					1189:1199	bone tissue engineering	1177:1199	bone tissue engineering	1177:1199	Hence, the novel fabrication idea and the obtained results suggested that the rCL/CS composite hydrogel scaffolds could be a promising three-dimensional bio-scaffold for bone tissue engineering.
33142583	4	46	theme	rCL/CS	482:487	arg1	scaffold					499:506	As-prepared rCL/CS composite scaffold	470:506	As-prepared rCL/CS composite scaffold	470:506	As-prepared rCL/CS composite scaffold showed unique porous morphology with rCL nanofibers imbibed CS matrix.
33142583	5	47	theme	compressive	583:593	arg1	test					604:607	The compressive strength test	579:607	The compressive strength test	579:607	The compressive strength test exhibited that the rCL/CS scaffold have higher compressive strength compared to pure CS.
33142583	3	48	theme	electrospun	423:433	arg1	nanofibers					458:467	electrospun cellulose acetate (CA) nanofibers	423:467	electrospun cellulose acetate (CA) nanofibers	423:467	The rCL nanofibers were regenerated from deacetylation of electrospun cellulose acetate (CA) nanofibers.
33142583	8	49	theme	hydrogel	1102:1109	arg1	scaffolds					1111:1119	the rCL/CS composite hydrogel scaffolds	1081:1119	the rCL/CS composite hydrogel scaffolds	1081:1119	Hence, the novel fabrication idea and the obtained results suggested that the rCL/CS composite hydrogel scaffolds could be a promising three-dimensional bio-scaffold for bone tissue engineering.
33142583	8	49	theme	hydrogel	1102:1109	arg1	bio-scaffold					1160:1171	a promising three-dimensional bio-scaffold	1130:1171	a promising three-dimensional bio-scaffold for bone tissue engineering	1130:1199	Hence, the novel fabrication idea and the obtained results suggested that the rCL/CS composite hydrogel scaffolds could be a promising three-dimensional bio-scaffold for bone tissue engineering.
33142583	5	50	theme	strength	595:602	arg1	test					604:607	The compressive strength test	579:607	The compressive strength test	579:607	The compressive strength test exhibited that the rCL/CS scaffold have higher compressive strength compared to pure CS.
33142583	1	51	theme	insufficient	143:154	arg1	strength					167:174	insufficient mechanical strength	143:174	insufficient mechanical strength which remains a major challenge in bone tissue engineering	143:233	Natural hydrogel scaffolds usually exhibit insufficient mechanical strength which remains a major challenge in bone tissue engineering.
33142583	6	52	theme	rCL/CS	702:707	arg1	scaffold					709:716	The rCL/CS scaffold	698:716	The rCL/CS scaffold	698:716	The rCL/CS scaffold showed increased biomineralization and enhanced pre-osteoblast cell (MC3T3-E1) viability, attachment, and proliferation.
33142583	7	53	theme	rCL/CS	980:985	arg1	scaffold					997:1004	rCL/CS composite scaffold	980:1004	rCL/CS composite scaffold	980:1004	The alkaline phosphatase (ALP) and alizarin red (ARS) staining results suggested that the osteogenic differentiation ability was improved in rCL/CS composite scaffold.
33142583	8	54	theme	obtained	1049:1056	arg1	results					1058:1064	the obtained results	1045:1064	the obtained results	1045:1064	Hence, the novel fabrication idea and the obtained results suggested that the rCL/CS composite hydrogel scaffolds could be a promising three-dimensional bio-scaffold for bone tissue engineering.
33142583	1	55	theme	mechanical	156:165	arg1	strength					167:174	insufficient mechanical strength	143:174	insufficient mechanical strength which remains a major challenge in bone tissue engineering	143:233	Natural hydrogel scaffolds usually exhibit insufficient mechanical strength which remains a major challenge in bone tissue engineering.
33142583	8	56	theme	tissue	1182:1187	arg1	engineering					1189:1199	bone tissue engineering	1177:1199	bone tissue engineering	1177:1199	Hence, the novel fabrication idea and the obtained results suggested that the rCL/CS composite hydrogel scaffolds could be a promising three-dimensional bio-scaffold for bone tissue engineering.
33142583	4	57	with	morphology	529:538	arg1	matrix					571:576	rCL nanofibers imbibed CS matrix	545:576	rCL nanofibers imbibed CS matrix	545:576	As-prepared rCL/CS composite scaffold showed unique porous morphology with rCL nanofibers imbibed CS matrix.
33142583	7	58	theme	differentiation	940:954	arg1	ability					956:962	the osteogenic differentiation ability	925:962	the osteogenic differentiation ability	925:962	The alkaline phosphatase (ALP) and alizarin red (ARS) staining results suggested that the osteogenic differentiation ability was improved in rCL/CS composite scaffold.
33142583	7	59	theme	ARS	888:890	arg1	staining					893:900	alizarin red (ARS) staining	874:900	alizarin red (ARS) staining	874:900	The alkaline phosphatase (ALP) and alizarin red (ARS) staining results suggested that the osteogenic differentiation ability was improved in rCL/CS composite scaffold.
33142583	0	60	theme	tissue	80:85	arg1	engineering					87:97	bone tissue engineering	75:97	bone tissue engineering	75:97	Regenerated cellulose nanofiber reinforced chitosan hydrogel scaffolds for bone tissue engineering.
33142583	0	61	theme	bone	75:78	arg1	engineering					87:97	bone tissue engineering	75:97	bone tissue engineering	75:97	Regenerated cellulose nanofiber reinforced chitosan hydrogel scaffolds for bone tissue engineering.
33142583	1	62	from	challenge	198:206	arg1	engineering					223:233	bone tissue engineering	211:233	bone tissue engineering	211:233	Natural hydrogel scaffolds usually exhibit insufficient mechanical strength which remains a major challenge in bone tissue engineering.
33142583	8	63	theme	three-dimensional	1142:1158	arg1	scaffolds					1111:1119	the rCL/CS composite hydrogel scaffolds	1081:1119	the rCL/CS composite hydrogel scaffolds	1081:1119	Hence, the novel fabrication idea and the obtained results suggested that the rCL/CS composite hydrogel scaffolds could be a promising three-dimensional bio-scaffold for bone tissue engineering.
33142583	8	63	theme	three-dimensional	1142:1158	arg1	bio-scaffold					1160:1171	a promising three-dimensional bio-scaffold	1130:1171	a promising three-dimensional bio-scaffold for bone tissue engineering	1130:1199	Hence, the novel fabrication idea and the obtained results suggested that the rCL/CS composite hydrogel scaffolds could be a promising three-dimensional bio-scaffold for bone tissue engineering.
33142583	7	64	theme	alkaline	843:850	arg1	ALP					865:867	ALP	865:867	ALP	865:867	The alkaline phosphatase (ALP) and alizarin red (ARS) staining results suggested that the osteogenic differentiation ability was improved in rCL/CS composite scaffold.
33142583	7	64	theme	alkaline	843:850	arg1	phosphatase					852:862	alkaline phosphatase	843:862	alkaline phosphatase (ALP)	843:868	The alkaline phosphatase (ALP) and alizarin red (ARS) staining results suggested that the osteogenic differentiation ability was improved in rCL/CS composite scaffold.
34181999	11	0	contain	had	1798:1800	arg2	peak					1806:1809	the peak	1802:1809	the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event	1802:2027	Films with KL had the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event.
34181999	11	0	contain	had	1798:1800	arg1	Films					1784:1788	Films	1784:1788	Films with KL	1784:1796	Films with KL had the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event.
34181999	11	1	theme	starch	1868:1873	arg1	film					1875:1878	the starch film	1864:1878	the starch film	1864:1878	Films with KL had the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event.
34181999	12	2	theme	modulus	2308:2314	arg1	values					2316:2321	storage modulus values	2300:2321	storage modulus values	2300:2321	There was also in the percentage of residue as the addition of KL was increased The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS.
34181999	11	3	from	peak	1925:1928	arg1	TPS					1937:1939	the TPS	1933:1939	the TPS	1933:1939	Films with KL had the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event.
34181999	3	4	theme	final	608:612	arg1	compounds					614:622	the final compounds	604:622	the final compounds	604:622	Thus, Kraft Lignin (KL), can be an excellent filler to be incorporated, since it presents mechanical and thermal properties and reduces the cost and weight of the final compounds.
34181999	2	5	theme	natural	330:336	arg1	origin					338:343	natural origin	330:343	natural origin	330:343	In this sense, the incorporation of fillers or reinforcements coming preferably from other substances of natural origin, can be an alternative to try to improve some mechanical and thermal properties of starch polymers.
34181999	12	6	from	TPS	2343:2345	arg1	TPS					2326:2328	TPS	2326:2328	TPS	2326:2328	There was also in the percentage of residue as the addition of KL was increased The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS.
34181999	7	7	theme	bands	1306:1310	arg1	intensity					1287:1295	the intensity	1283:1295	the intensity of these bands varying among the samples studied	1283:1344	The FT-IR spectra of the films showed bands typical of functional groups derived from starch and lignin, with the intensity of these bands varying among the samples studied.
34181999	12	8	theme	TPS	2178:2180	arg1	film					2182:2185	TPS film	2178:2185	TPS film	2178:2185	There was also in the percentage of residue as the addition of KL was increased The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS.
34181999	10	9	theme	TPS	1740:1742	arg1	event					1683:1687	the main degradation event	1662:1687	the main degradation event	1662:1687	In the TGA analysis, it was possible to observe a change in the main degradation event of the films for lower temperatures, especially of TPS - 4 and 8% KL compared to the TPS film.
34181999	3	10	theme	excellent	480:488	arg1	Lignin					457:462	Kraft Lignin	451:462	Kraft Lignin (KL)	451:467	Thus, Kraft Lignin (KL), can be an excellent filler to be incorporated, since it presents mechanical and thermal properties and reduces the cost and weight of the final compounds.
34181999	3	10	theme	excellent	480:488	arg1	filler					490:495	an excellent filler	477:495	an excellent filler	477:495	Thus, Kraft Lignin (KL), can be an excellent filler to be incorporated, since it presents mechanical and thermal properties and reduces the cost and weight of the final compounds.
34181999	12	11	theme	KL	2172:2173	arg1	presence					2160:2167	presence	2160:2167	presence of KL in TPS film	2160:2185	There was also in the percentage of residue as the addition of KL was increased The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS.
34181999	6	12	dep	Spectroscopy	1150:1161	arg1	UV-Vis					1164:1169	UV-Vis	1164:1169	UV-Vis	1164:1169	Samples were also analyzed for biodegradation and for the ability to remove contaminants in water, Metil Orange (MO), by Ultraviolet-Visible Spectroscopy (UV-Vis).
34181999	13	13	contain	has	2399:2401	arg1	KL					2396:2397	4% KL	2393:2397	4% KL	2393:2397	For the tensile strength property only TPS - 4% KL has significant improvement, and the elongation at break showed an increase for TPS - 4 and 8% KL compared to TPS.
34181999	13	13	contain	has	2399:2401	arg2	improvement					2415:2425	significant improvement	2403:2425	significant improvement	2403:2425	For the tensile strength property only TPS - 4% KL has significant improvement, and the elongation at break showed an increase for TPS - 4 and 8% KL compared to TPS.
34181999	5	14	theme	films	762:766	arg1	Characterization					738:753	Characterization	738:753	Characterization of TPS films and compositions with KL	738:791	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	7	15	theme	functional	1228:1237	arg1	groups					1239:1244	functional groups	1228:1244	functional groups derived from starch and lignin	1228:1275	The FT-IR spectra of the films showed bands typical of functional groups derived from starch and lignin, with the intensity of these bands varying among the samples studied.
34181999	5	16	theme	Dynamic	932:938	arg1	DMTA					967:970	DMTA	967:970	DMTA	967:970	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	5	16	theme	Dynamic	932:938	arg1	Analysis					957:964	Dynamic Thermomechanical Analysis	932:964	Dynamic Thermomechanical Analysis (DMTA)	932:971	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	12	17	theme	%	2333:2333	arg1	KL					2335:2336	8% KL	2332:2336	8% KL	2332:2336	There was also in the percentage of residue as the addition of KL was increased The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS.
34181999	7	18	theme	typical	1217:1223	arg1	bands					1211:1215	bands	1211:1215	bands typical of functional groups derived from starch and lignin	1211:1275	The FT-IR spectra of the films showed bands typical of functional groups derived from starch and lignin, with the intensity of these bands varying among the samples studied.
34181999	14	19	theme	biodegradation	2558:2571	arg1	process					2573:2579	a continuous and progressive biodegradation process	2529:2579	a continuous and progressive biodegradation process	2529:2579	Samples showed a continuous and progressive biodegradation process, being completely biodegraded within 10 days.
34181999	9	20	theme	angle	1551:1555	arg1	values					1557:1562	contact angle values	1543:1562	contact angle values	1543:1562	Increasing the percentage of KL led to an increase in contact angle values, showing a more hydrophobic behavior.
34181999	10	21	theme	films	1696:1700	arg1	event					1683:1687	the main degradation event	1662:1687	the main degradation event	1662:1687	In the TGA analysis, it was possible to observe a change in the main degradation event of the films for lower temperatures, especially of TPS - 4 and 8% KL compared to the TPS film.
34181999	13	22	dep	showed	2456:2461	arg1	compared					2497:2504	compared	2497:2504	showed an increase for TPS - 4 and 8% KL compared to TPS	2456:2511	For the tensile strength property only TPS - 4% KL has significant improvement, and the elongation at break showed an increase for TPS - 4 and 8% KL compared to TPS.
34181999	11	23	theme	degradation	1822:1832	arg1	peak					1806:1809	the peak	1802:1809	the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event	1802:2027	Films with KL had the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event.
34181999	6	24	theme	Ultraviolet-Visible	1130:1148	arg1	Spectroscopy					1150:1161	Ultraviolet-Visible Spectroscopy	1130:1161	Ultraviolet-Visible Spectroscopy (UV-Vis)	1130:1170	Samples were also analyzed for biodegradation and for the ability to remove contaminants in water, Metil Orange (MO), by Ultraviolet-Visible Spectroscopy (UV-Vis).
34181999	13	25	theme	significant	2403:2413	arg1	improvement					2415:2425	significant improvement	2403:2425	significant improvement	2403:2425	For the tensile strength property only TPS - 4% KL has significant improvement, and the elongation at break showed an increase for TPS - 4 and 8% KL compared to TPS.
34181999	11	26	theme	4	1943:1943	arg1	%					1944:1944	%	1944:1944	%	1944:1944	Films with KL had the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event.
34181999	10	27	theme	TPS	1774:1776	arg1	film					1778:1781	the TPS film	1770:1781	the TPS film	1770:1781	In the TGA analysis, it was possible to observe a change in the main degradation event of the films for lower temperatures, especially of TPS - 4 and 8% KL compared to the TPS film.
34181999	14	28	theme	progressive	2546:2556	arg1	process					2573:2579	a continuous and progressive biodegradation process	2529:2579	a continuous and progressive biodegradation process	2529:2579	Samples showed a continuous and progressive biodegradation process, being completely biodegraded within 10 days.
34181999	5	29	theme	compositions	772:783	arg1	Characterization					738:753	Characterization	738:753	Characterization of TPS films and compositions with KL	738:791	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	15	30	theme	promising	2714:2722	arg1	results					2724:2730	promising results	2714:2730	promising results of compounds for this application	2714:2764	The monitoring of the ability to remove contaminants from water by UV-Vis, also showed promising results of compounds for this application.
34181999	9	31	theme	hydrophobic	1580:1590	arg1	behavior					1592:1599	a more hydrophobic behavior	1573:1599	a more hydrophobic behavior	1573:1599	Increasing the percentage of KL led to an increase in contact angle values, showing a more hydrophobic behavior.
34181999	10	32	dep	%	1753:1753	arg1	KL					1755:1756	KL	1755:1756	4 and 8% KL	1746:1756	In the TGA analysis, it was possible to observe a change in the main degradation event of the films for lower temperatures, especially of TPS - 4 and 8% KL compared to the TPS film.
34181999	4	33	theme	TPS	625:627	arg1	films					629:633	TPS films	625:633	TPS films	625:633	TPS films were prepared by casting using dimethyl sulfoxide (DMSO) as solvent and additives with 2, 4 and 8% KL.
34181999	13	34	dep	KL	2494:2495	arg1	%					2492:2492	%	2492:2492	%	2492:2492	For the tensile strength property only TPS - 4% KL has significant improvement, and the elongation at break showed an increase for TPS - 4 and 8% KL compared to TPS.
34181999	0	35	theme	thermoplastic	73:85	arg1	starch					87:92	thermoplastic starch	73:92	thermoplastic starch	73:92	Improvements in thermal and mechanical properties of composites based on thermoplastic starch and Kraft Lignin.
34181999	5	36	theme	contact	994:1000	arg1	angle					1002:1006	contact angle	994:1006	contact angle	994:1006	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	13	37	theme	4	2393:2393	arg1	%					2394:2394	%	2394:2394	%	2394:2394	For the tensile strength property only TPS - 4% KL has significant improvement, and the elongation at break showed an increase for TPS - 4 and 8% KL compared to TPS.
34181999	0	38	from	Improvements	0:11	arg1	properties					39:48	thermal and mechanical properties	16:48	thermal and mechanical properties of composites	16:62	Improvements in thermal and mechanical properties of composites based on thermoplastic starch and Kraft Lignin.
34181999	12	39	from	presence	2160:2167	arg1	film					2182:2185	TPS film	2178:2185	TPS film	2178:2185	There was also in the percentage of residue as the addition of KL was increased The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS.
34181999	9	40	from	increase	1531:1538	arg1	values					1557:1562	contact angle values	1543:1562	contact angle values	1543:1562	Increasing the percentage of KL led to an increase in contact angle values, showing a more hydrophobic behavior.
34181999	11	41	theme	KL	1946:1947	arg1	intensity					1903:1911	intensity	1903:1911	intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples	1903:1971	Films with KL had the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event.
34181999	16	42	theme	KL	2830:2831	arg1	films					2833:2837	the TPS- 4% KL films	2818:2837	the TPS- 4% KL films	2818:2837	The best results were obtained, in most tests, for the TPS- 4% KL films.
34181999	5	43	theme	Scanning	862:869	arg1	SEM					892:894	SEM	892:894	SEM	892:894	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	5	43	theme	Scanning	862:869	arg1	Microscope					880:889	Scanning Electron Microscope	862:889	Scanning Electron Microscope (SEM)	862:895	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	7	44	theme	FT-IR	1177:1181	arg1	spectra					1183:1189	The FT-IR spectra	1173:1189	The FT-IR spectra of the films	1173:1202	The FT-IR spectra of the films showed bands typical of functional groups derived from starch and lignin, with the intensity of these bands varying among the samples studied.
34181999	4	45	dep	KL	734:735	arg1	%					732:732	%	732:732	%	732:732	TPS films were prepared by casting using dimethyl sulfoxide (DMSO) as solvent and additives with 2, 4 and 8% KL.
34181999	15	46	from	water	2685:2689	arg1	contaminants					2667:2678	contaminants	2667:2678	contaminants from water	2667:2689	The monitoring of the ability to remove contaminants from water by UV-Vis, also showed promising results of compounds for this application.
34181999	11	47	theme	main	1920:1923	arg1	peak					1925:1928	the main peak	1916:1928	the main peak in the TPS	1916:1939	Films with KL had the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event.
34181999	5	48	theme	Infrared	831:838	arg1	Spectroscopy					840:851	Fourier-Transform Infrared Spectroscopy	813:851	Fourier-Transform Infrared Spectroscopy (FT-IR)	813:859	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	5	48	theme	Infrared	831:838	arg1	FT-IR					854:858	FT-IR	854:858	FT-IR	854:858	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	12	49	theme	energy	2218:2223	arg1	property					2233:2240	its energy storage property	2214:2240	its energy storage property	2214:2240	There was also in the percentage of residue as the addition of KL was increased The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS.
34181999	0	50	theme	thermal	16:22	arg1	properties					39:48	thermal and mechanical properties	16:48	thermal and mechanical properties of composites	16:62	Improvements in thermal and mechanical properties of composites based on thermoplastic starch and Kraft Lignin.
34181999	13	51	from	break	2450:2454	arg1	elongation					2436:2445	the elongation	2432:2445	the elongation at break	2432:2454	For the tensile strength property only TPS - 4% KL has significant improvement, and the elongation at break showed an increase for TPS - 4 and 8% KL compared to TPS.
34181999	0	52	theme	mechanical	28:37	arg1	properties					39:48	thermal and mechanical properties	16:48	thermal and mechanical properties of composites	16:62	Improvements in thermal and mechanical properties of composites based on thermoplastic starch and Kraft Lignin.
34181999	14	53	theme	continuous	2531:2540	arg1	process					2573:2579	a continuous and progressive biodegradation process	2529:2579	a continuous and progressive biodegradation process	2529:2579	Samples showed a continuous and progressive biodegradation process, being completely biodegraded within 10 days.
34181999	2	54	theme	starch	428:433	arg1	polymers					435:442	starch polymers	428:442	starch polymers	428:442	In this sense, the incorporation of fillers or reinforcements coming preferably from other substances of natural origin, can be an alternative to try to improve some mechanical and thermal properties of starch polymers.
34181999	11	55	dep	KL	1946:1947	arg1	samples					1965:1971	KL samples	1962:1971	KL samples	1962:1971	Films with KL had the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event.
34181999	3	56	theme	Kraft	451:455	arg1	KL					465:466	KL	465:466	KL	465:466	Thus, Kraft Lignin (KL), can be an excellent filler to be incorporated, since it presents mechanical and thermal properties and reduces the cost and weight of the final compounds.
34181999	3	56	theme	Kraft	451:455	arg1	Lignin					457:462	Kraft Lignin	451:462	Kraft Lignin (KL)	451:467	Thus, Kraft Lignin (KL), can be an excellent filler to be incorporated, since it presents mechanical and thermal properties and reduces the cost and weight of the final compounds.
34181999	3	56	theme	Kraft	451:455	arg1	filler					490:495	an excellent filler	477:495	an excellent filler	477:495	Thus, Kraft Lignin (KL), can be an excellent filler to be incorporated, since it presents mechanical and thermal properties and reduces the cost and weight of the final compounds.
34181999	2	57	theme	other	310:314	arg1	substances					316:325	other substances	310:325	other substances of natural origin	310:343	In this sense, the incorporation of fillers or reinforcements coming preferably from other substances of natural origin, can be an alternative to try to improve some mechanical and thermal properties of starch polymers.
34181999	15	58	theme	compounds	2735:2743	arg1	results					2724:2730	promising results	2714:2730	promising results of compounds for this application	2714:2764	The monitoring of the ability to remove contaminants from water by UV-Vis, also showed promising results of compounds for this application.
34181999	8	59	theme	irregular	1432:1440	arg1	shapes					1442:1447	irregular shapes	1432:1447	irregular shapes with structures that appeared as plots	1432:1486	Micrographs revealed slightly different morphologies among the films, but all showed irregular shapes with structures that appeared as plots.
34181999	10	60	theme	degradation	1671:1681	arg1	event					1683:1687	the main degradation event	1662:1687	the main degradation event	1662:1687	In the TGA analysis, it was possible to observe a change in the main degradation event of the films for lower temperatures, especially of TPS - 4 and 8% KL compared to the TPS film.
34181999	1	61	theme	polymers	215:222	arg1	use					195:197	the use	191:197	the use of conventional polymers	191:222	Thermoplastic starch (TPS) is a widely studied biopolymer as an alternative to the use of conventional polymers.
34181999	10	62	from	change	1652:1657	arg1	event					1683:1687	the main degradation event	1662:1687	the main degradation event	1662:1687	In the TGA analysis, it was possible to observe a change in the main degradation event of the films for lower temperatures, especially of TPS - 4 and 8% KL compared to the TPS film.
34181999	12	63	theme	residue	2066:2072	arg1	percentage					2052:2061	the percentage	2048:2061	the percentage of residue	2048:2072	There was also in the percentage of residue as the addition of KL was increased The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS.
34181999	10	64	theme	lower	1706:1710	arg1	temperatures					1712:1723	lower temperatures	1706:1723	lower temperatures	1706:1723	In the TGA analysis, it was possible to observe a change in the main degradation event of the films for lower temperatures, especially of TPS - 4 and 8% KL compared to the TPS film.
34181999	2	65	theme	origin	338:343	arg1	substances					316:325	other substances	310:325	other substances of natural origin	310:343	In this sense, the incorporation of fillers or reinforcements coming preferably from other substances of natural origin, can be an alternative to try to improve some mechanical and thermal properties of starch polymers.
34181999	3	66	theme	compounds	614:622	arg1	weight					594:599	weight	594:599	weight	594:599	Thus, Kraft Lignin (KL), can be an excellent filler to be incorporated, since it presents mechanical and thermal properties and reduces the cost and weight of the final compounds.
34181999	3	66	theme	compounds	614:622	arg1	cost					585:588	cost	585:588	cost	585:588	Thus, Kraft Lignin (KL), can be an excellent filler to be incorporated, since it presents mechanical and thermal properties and reduces the cost and weight of the final compounds.
34181999	2	67	theme	fillers	261:267	arg1	alternative					356:366	an alternative	353:366	an alternative to try to improve some mechanical and thermal properties of starch polymers	353:442	In this sense, the incorporation of fillers or reinforcements coming preferably from other substances of natural origin, can be an alternative to try to improve some mechanical and thermal properties of starch polymers.
34181999	2	67	theme	fillers	261:267	arg1	incorporation					244:256	the incorporation	240:256	the incorporation	240:256	In this sense, the incorporation of fillers or reinforcements coming preferably from other substances of natural origin, can be an alternative to try to improve some mechanical and thermal properties of starch polymers.
34181999	11	68	theme	KL	1962:1963	arg1	samples					1965:1971	KL samples	1962:1971	KL samples	1962:1971	Films with KL had the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event.
34181999	11	69	from	intensity	1903:1911	arg1	TPS					1937:1939	the TPS	1933:1939	the TPS	1933:1939	Films with KL had the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event.
34181999	8	70	with	shapes	1442:1447	arg1	structures					1454:1463	structures	1454:1463	structures that appeared as plots	1454:1486	Micrographs revealed slightly different morphologies among the films, but all showed irregular shapes with structures that appeared as plots.
34181999	1	71	theme	studied	151:157	arg1	biopolymer					159:168	a widely studied biopolymer	142:168	a widely studied biopolymer as an alternative to the use of conventional polymers	142:222	Thermoplastic starch (TPS) is a widely studied biopolymer as an alternative to the use of conventional polymers.
34181999	1	71	theme	studied	151:157	arg1	starch					126:131	Thermoplastic starch	112:131	Thermoplastic starch (TPS)	112:137	Thermoplastic starch (TPS) is a widely studied biopolymer as an alternative to the use of conventional polymers.
34181999	9	72	theme	KL	1518:1519	arg1	percentage					1504:1513	the percentage	1500:1513	the percentage of KL	1500:1519	Increasing the percentage of KL led to an increase in contact angle values, showing a more hydrophobic behavior.
34181999	6	73	theme	Metil	1108:1112	arg1	Orange					1114:1119	Metil Orange	1108:1119	Metil Orange (MO)	1108:1124	Samples were also analyzed for biodegradation and for the ability to remove contaminants in water, Metil Orange (MO), by Ultraviolet-Visible Spectroscopy (UV-Vis).
34181999	6	73	theme	Metil	1108:1112	arg1	MO					1122:1123	MO	1122:1123	MO	1122:1123	Samples were also analyzed for biodegradation and for the ability to remove contaminants in water, Metil Orange (MO), by Ultraviolet-Visible Spectroscopy (UV-Vis).
34181999	6	73	theme	Metil	1108:1112	arg1	water					1101:1105	water	1101:1105	water	1101:1105	Samples were also analyzed for biodegradation and for the ability to remove contaminants in water, Metil Orange (MO), by Ultraviolet-Visible Spectroscopy (UV-Vis).
34181999	10	74	theme	TGA	1609:1611	arg1	analysis					1613:1620	the TGA analysis	1605:1620	the TGA analysis	1605:1620	In the TGA analysis, it was possible to observe a change in the main degradation event of the films for lower temperatures, especially of TPS - 4 and 8% KL compared to the TPS film.
34181999	5	75	theme	TPS	758:760	arg1	films					762:766	TPS films	758:766	TPS films	758:766	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	16	76	theme	%	2828:2828	arg1	films					2833:2837	the TPS- 4% KL films	2818:2837	the TPS- 4% KL films	2818:2837	The best results were obtained, in most tests, for the TPS- 4% KL films.
34181999	7	77	attach	derived	1246:1252	arg1	lignin					1270:1275	lignin	1270:1275	lignin	1270:1275	The FT-IR spectra of the films showed bands typical of functional groups derived from starch and lignin, with the intensity of these bands varying among the samples studied.
34181999	7	77	attach	derived	1246:1252	arg1	starch					1259:1264	starch	1259:1264	starch	1259:1264	The FT-IR spectra of the films showed bands typical of functional groups derived from starch and lignin, with the intensity of these bands varying among the samples studied.
34181999	7	77	attach	derived	1246:1252	arg2	groups					1239:1244	functional groups	1228:1244	functional groups derived from starch and lignin	1228:1275	The FT-IR spectra of the films showed bands typical of functional groups derived from starch and lignin, with the intensity of these bands varying among the samples studied.
34181999	2	78	theme	reinforcements	272:285	arg1	alternative					356:366	an alternative	353:366	an alternative to try to improve some mechanical and thermal properties of starch polymers	353:442	In this sense, the incorporation of fillers or reinforcements coming preferably from other substances of natural origin, can be an alternative to try to improve some mechanical and thermal properties of starch polymers.
34181999	2	78	theme	reinforcements	272:285	arg1	incorporation					244:256	the incorporation	240:256	the incorporation	240:256	In this sense, the incorporation of fillers or reinforcements coming preferably from other substances of natural origin, can be an alternative to try to improve some mechanical and thermal properties of starch polymers.
34181999	11	79	theme	maximum	1814:1820	arg1	degradation					1822:1832	maximum degradation	1814:1832	maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event	1814:2027	Films with KL had the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event.
34181999	7	80	theme	groups	1239:1244	arg1	typical					1217:1223	typical	1217:1223	typical	1217:1223	The FT-IR spectra of the films showed bands typical of functional groups derived from starch and lignin, with the intensity of these bands varying among the samples studied.
34181999	11	81	from	decrease	1891:1898	arg1	intensity					1903:1911	intensity	1903:1911	intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples	1903:1971	Films with KL had the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event.
34181999	5	82	theme	Thermomechanical	940:955	arg1	DMTA					967:970	DMTA	967:970	DMTA	967:970	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	5	82	theme	Thermomechanical	940:955	arg1	Analysis					957:964	Dynamic Thermomechanical Analysis	932:964	Dynamic Thermomechanical Analysis (DMTA)	932:971	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	15	83	theme	ability	2649:2655	arg1	monitoring					2631:2640	The monitoring	2627:2640	The monitoring of the ability to remove contaminants from water by UV-Vis, also	2627:2705	The monitoring of the ability to remove contaminants from water by UV-Vis, also showed promising results of compounds for this application.
34181999	9	84	theme	contact	1543:1549	arg1	values					1557:1562	contact angle values	1543:1562	contact angle values	1543:1562	Increasing the percentage of KL led to an increase in contact angle values, showing a more hydrophobic behavior.
34181999	12	85	theme	decreasing	2280:2289	arg1	order					2291:2295	a decreasing order	2278:2295	a decreasing order of storage modulus values to TPS - 8% KL from TPS	2278:2345	There was also in the percentage of residue as the addition of KL was increased The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS.
34181999	1	86	theme	Thermoplastic	112:124	arg1	TPS					134:136	TPS	134:136	TPS	134:136	Thermoplastic starch (TPS) is a widely studied biopolymer as an alternative to the use of conventional polymers.
34181999	1	86	theme	Thermoplastic	112:124	arg1	starch					126:131	Thermoplastic starch	112:131	Thermoplastic starch (TPS)	112:137	Thermoplastic starch (TPS) is a widely studied biopolymer as an alternative to the use of conventional polymers.
34181999	1	86	theme	Thermoplastic	112:124	arg1	biopolymer					159:168	a widely studied biopolymer	142:168	a widely studied biopolymer as an alternative to the use of conventional polymers	142:222	Thermoplastic starch (TPS) is a widely studied biopolymer as an alternative to the use of conventional polymers.
34181999	16	87	theme	4	2827:2827	arg1	%					2828:2828	%	2828:2828	%	2828:2828	The best results were obtained, in most tests, for the TPS- 4% KL films.
34181999	12	88	theme	storage	2300:2306	arg1	modulus					2308:2314	storage modulus	2300:2314	storage modulus values	2300:2321	There was also in the percentage of residue as the addition of KL was increased The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS.
34181999	6	89	from	contaminants	1085:1096	arg1	Orange					1114:1119	Metil Orange	1108:1119	Metil Orange (MO)	1108:1124	Samples were also analyzed for biodegradation and for the ability to remove contaminants in water, Metil Orange (MO), by Ultraviolet-Visible Spectroscopy (UV-Vis).
34181999	6	89	from	contaminants	1085:1096	arg1	water					1101:1105	water	1101:1105	water	1101:1105	Samples were also analyzed for biodegradation and for the ability to remove contaminants in water, Metil Orange (MO), by Ultraviolet-Visible Spectroscopy (UV-Vis).
34181999	3	90	theme	thermal	550:556	arg1	properties					558:567	mechanical and thermal properties	535:567	mechanical and thermal properties	535:567	Thus, Kraft Lignin (KL), can be an excellent filler to be incorporated, since it presents mechanical and thermal properties and reduces the cost and weight of the final compounds.
34181999	12	91	theme	values	2316:2321	arg1	order					2291:2295	a decreasing order	2278:2295	a decreasing order of storage modulus values to TPS - 8% KL from TPS	2278:2345	There was also in the percentage of residue as the addition of KL was increased The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS.
34181999	7	92	theme	films	1198:1202	arg1	spectra					1183:1189	The FT-IR spectra	1173:1189	The FT-IR spectra of the films	1173:1202	The FT-IR spectra of the films showed bands typical of functional groups derived from starch and lignin, with the intensity of these bands varying among the samples studied.
34181999	12	93	theme	loss	2256:2259	arg1	modulus					2261:2267	the loss modulus	2252:2267	the loss modulus	2252:2267	There was also in the percentage of residue as the addition of KL was increased The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS.
34181999	3	94	theme	mechanical	535:544	arg1	properties					558:567	mechanical and thermal properties	535:567	mechanical and thermal properties	535:567	Thus, Kraft Lignin (KL), can be an excellent filler to be incorporated, since it presents mechanical and thermal properties and reduces the cost and weight of the final compounds.
34181999	16	95	theme	most	2802:2805	arg1	tests					2807:2811	most tests	2802:2811	most tests	2802:2811	The best results were obtained, in most tests, for the TPS- 4% KL films.
34181999	11	96	theme	%	1944:1944	arg1	KL					1946:1947	4% KL	1943:1947	4% KL	1943:1947	Films with KL had the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event.
34181999	16	97	theme	TPS-	2822:2825	arg1	films					2833:2837	the TPS- 4% KL films	2818:2837	the TPS- 4% KL films	2818:2837	The best results were obtained, in most tests, for the TPS- 4% KL films.
34181999	12	98	attach	presence	2160:2167	arg2	KL					2172:2173	KL	2172:2173	KL	2172:2173	There was also in the percentage of residue as the addition of KL was increased The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS.
34181999	12	98	attach	presence	2160:2167	arg1	film					2182:2185	TPS film	2178:2185	TPS film	2178:2185	There was also in the percentage of residue as the addition of KL was increased The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS.
34181999	4	99	with	additives	707:715	arg1	KL					734:735	2, 4 and 8% KL	722:735	2, 4 and 8% KL	722:735	TPS films were prepared by casting using dimethyl sulfoxide (DMSO) as solvent and additives with 2, 4 and 8% KL.
34181999	0	100	theme	Kraft	98:102	arg1	Lignin					104:109	Kraft Lignin	98:109	Kraft Lignin	98:109	Improvements in thermal and mechanical properties of composites based on thermoplastic starch and Kraft Lignin.
34181999	13	101	theme	%	2394:2394	arg1	KL					2396:2397	4% KL	2393:2397	4% KL	2393:2397	For the tensile strength property only TPS - 4% KL has significant improvement, and the elongation at break showed an increase for TPS - 4 and 8% KL compared to TPS.
34181999	5	102	with	compositions	772:783	arg1	KL					790:791	KL	790:791	KL	790:791	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	4	103	with	solvent	695:701	arg1	KL					734:735	2, 4 and 8% KL	722:735	2, 4 and 8% KL	722:735	TPS films were prepared by casting using dimethyl sulfoxide (DMSO) as solvent and additives with 2, 4 and 8% KL.
34181999	10	104	from	analysis	1613:1620	arg1	possible					1630:1637	possible	1630:1637	possible	1630:1637	In the TGA analysis, it was possible to observe a change in the main degradation event of the films for lower temperatures, especially of TPS - 4 and 8% KL compared to the TPS film.
34181999	12	105	theme	8	2332:2332	arg1	%					2333:2333	%	2333:2333	%	2333:2333	There was also in the percentage of residue as the addition of KL was increased The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS.
34181999	13	106	dep	%	2492:2492	arg1	8					2491:2491	8	2491:2491	8	2491:2491	For the tensile strength property only TPS - 4% KL has significant improvement, and the elongation at break showed an increase for TPS - 4 and 8% KL compared to TPS.
34181999	13	106	dep	%	2492:2492	arg1	4					2485:2485	4	2485:2485	4	2485:2485	For the tensile strength property only TPS - 4% KL has significant improvement, and the elongation at break showed an increase for TPS - 4 and 8% KL compared to TPS.
34181999	8	107	theme	different	1377:1385	arg1	morphologies					1387:1398	slightly different morphologies	1368:1398	slightly different morphologies	1368:1398	Micrographs revealed slightly different morphologies among the films, but all showed irregular shapes with structures that appeared as plots.
34181999	11	108	with	Films	1784:1788	arg1	KL					1795:1796	KL	1795:1796	KL	1795:1796	Films with KL had the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event.
34181999	5	109	theme	tensile	974:980	arg1	testing					982:988	tensile testing	974:988	tensile testing	974:988	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	5	110	theme	Fourier-Transform	813:829	arg1	Spectroscopy					840:851	Fourier-Transform Infrared Spectroscopy	813:851	Fourier-Transform Infrared Spectroscopy (FT-IR)	813:859	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	5	110	theme	Fourier-Transform	813:829	arg1	FT-IR					854:858	FT-IR	854:858	FT-IR	854:858	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	2	111	theme	thermal	406:412	arg1	properties					414:423	some mechanical and thermal properties	386:423	some mechanical and thermal properties of starch polymers	386:442	In this sense, the incorporation of fillers or reinforcements coming preferably from other substances of natural origin, can be an alternative to try to improve some mechanical and thermal properties of starch polymers.
34181999	12	112	theme	storage	2225:2231	arg1	property					2233:2240	its energy storage property	2214:2240	its energy storage property	2214:2240	There was also in the percentage of residue as the addition of KL was increased The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS.
34181999	4	113	dep	%	732:732	arg1	8					731:731	8	731:731	8	731:731	TPS films were prepared by casting using dimethyl sulfoxide (DMSO) as solvent and additives with 2, 4 and 8% KL.
34181999	4	113	dep	%	732:732	arg1	4					725:725	4	725:725	4	725:725	TPS films were prepared by casting using dimethyl sulfoxide (DMSO) as solvent and additives with 2, 4 and 8% KL.
34181999	16	114	theme	best	2771:2774	arg1	results					2776:2782	The best results	2767:2782	The best results	2767:2782	The best results were obtained, in most tests, for the TPS- 4% KL films.
34181999	10	115	from	possible	1630:1637	arg1	analysis					1613:1620	the TGA analysis	1605:1620	the TGA analysis	1605:1620	In the TGA analysis, it was possible to observe a change in the main degradation event of the films for lower temperatures, especially of TPS - 4 and 8% KL compared to the TPS film.
34181999	2	116	theme	mechanical	391:400	arg1	properties					414:423	some mechanical and thermal properties	386:423	some mechanical and thermal properties of starch polymers	386:442	In this sense, the incorporation of fillers or reinforcements coming preferably from other substances of natural origin, can be an alternative to try to improve some mechanical and thermal properties of starch polymers.
34181999	12	117	theme	DMTA	2114:2117	arg1	analyses					2119:2126	The DMTA analyses	2110:2126	The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS	2110:2345	There was also in the percentage of residue as the addition of KL was increased The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS.
34181999	10	118	theme	main	1666:1669	arg1	event					1683:1687	the main degradation event	1662:1687	the main degradation event	1662:1687	In the TGA analysis, it was possible to observe a change in the main degradation event of the films for lower temperatures, especially of TPS - 4 and 8% KL compared to the TPS film.
34181999	4	119	theme	dimethyl	666:673	arg1	additives					707:715	additives	707:715	additives with 2, 4 and 8% KL	707:735	TPS films were prepared by casting using dimethyl sulfoxide (DMSO) as solvent and additives with 2, 4 and 8% KL.
34181999	4	119	theme	dimethyl	666:673	arg1	DMSO					686:689	DMSO	686:689	DMSO	686:689	TPS films were prepared by casting using dimethyl sulfoxide (DMSO) as solvent and additives with 2, 4 and 8% KL.
34181999	4	119	theme	dimethyl	666:673	arg1	sulfoxide					675:683	dimethyl sulfoxide	666:683	dimethyl sulfoxide (DMSO)	666:690	TPS films were prepared by casting using dimethyl sulfoxide (DMSO) as solvent and additives with 2, 4 and 8% KL.
34181999	12	120	from	increase	2202:2209	arg1	property					2233:2240	its energy storage property	2214:2240	its energy storage property	2214:2240	There was also in the percentage of residue as the addition of KL was increased The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS.
34181999	2	121	theme	polymers	435:442	arg1	properties					414:423	some mechanical and thermal properties	386:423	some mechanical and thermal properties of starch polymers	386:442	In this sense, the incorporation of fillers or reinforcements coming preferably from other substances of natural origin, can be an alternative to try to improve some mechanical and thermal properties of starch polymers.
34181999	0	122	theme	composites	53:62	arg1	properties					39:48	thermal and mechanical properties	16:48	thermal and mechanical properties of composites	16:62	Improvements in thermal and mechanical properties of composites based on thermoplastic starch and Kraft Lignin.
34181999	5	123	theme	Thermogravimetric	898:914	arg1	TGA					926:928	TGA	926:928	TGA	926:928	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	5	123	theme	Thermogravimetric	898:914	arg1	Analysis					916:923	Thermogravimetric Analysis	898:923	Thermogravimetric Analysis (TGA)	898:929	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	11	124	theme	peak	1925:1928	arg1	intensity					1903:1911	intensity	1903:1911	intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples	1903:1971	Films with KL had the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event.
34181999	12	125	theme	KL	2093:2094	arg1	addition					2081:2088	the addition	2077:2088	the addition of KL	2077:2094	There was also in the percentage of residue as the addition of KL was increased The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS.
34181999	5	126	with	films	762:766	arg1	KL					790:791	KL	790:791	KL	790:791	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	11	127	from	TPS	1937:1939	arg1	intensity					1903:1911	intensity	1903:1911	intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples	1903:1971	Films with KL had the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event.
34181999	5	128	theme	Electron	871:878	arg1	SEM					892:894	SEM	892:894	SEM	892:894	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	5	128	theme	Electron	871:878	arg1	Microscope					880:889	Scanning Electron Microscope	862:889	Scanning Electron Microscope (SEM)	862:895	Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle.
34181999	2	129	from	alternative	356:366	arg1	sense					233:237	this sense	228:237	this sense	228:237	In this sense, the incorporation of fillers or reinforcements coming preferably from other substances of natural origin, can be an alternative to try to improve some mechanical and thermal properties of starch polymers.
34181999	13	130	theme	tensile	2356:2362	arg1	strength					2364:2371	the tensile strength	2352:2371	the tensile strength	2352:2371	For the tensile strength property only TPS - 4% KL has significant improvement, and the elongation at break showed an increase for TPS - 4 and 8% KL compared to TPS.
34181999	1	131	theme	conventional	202:213	arg1	polymers					215:222	conventional polymers	202:222	conventional polymers	202:222	Thermoplastic starch (TPS) is a widely studied biopolymer as an alternative to the use of conventional polymers.
34181999	11	132	theme	mass	2006:2009	arg1	loss					2011:2014	less mass loss	2001:2014	less mass loss	2001:2014	Films with KL had the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event.
34181999	3	133	dep	cost	585:588	arg1	the					581:583	the	581:583	the	581:583	Thus, Kraft Lignin (KL), can be an excellent filler to be incorporated, since it presents mechanical and thermal properties and reduces the cost and weight of the final compounds.
32422263	3	0	theme	GAG	362:364	arg1	disaccharides					366:378	GAG disaccharides	362:378	GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model	362:515	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	3	0	theme	GAG	362:364	arg1	sulfate					398:404	heparan sulfate	390:404	heparan sulfate (HS)	390:409	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	3	0	theme	GAG	362:364	arg1	sulfate					427:433	chondroitin sulfate	415:433	chondroitin sulfate (CS)	415:438	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	6	1	theme	CS	1119:1120	arg1	under-sulfation					1088:1102	under-sulfation	1088:1102	under-sulfation of both HS and CS disaccharides	1088:1134	At day 15, under-sulfation of both HS and CS disaccharides was persisted.
32422263	3	2	theme	lung	475:478	arg1	tissues					480:486	bleomycin-induced and control lung tissues	445:486	tissues	480:486	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	8	3	theme	defective	1483:1491	arg1	molecules					1503:1511	defective signaling molecules	1483:1511	defective signaling molecules involved in bleomycin-induced lung fibrosis	1483:1555	In conclusion, GAGs, especially HS, from fibrotic lungs induced by a single injection of bleomycin were significantly under-sulfated up to 30 days, suggesting GAGs might be another class of defective signaling molecules involved in bleomycin-induced lung fibrosis.
32422263	3	4	from	sulfate	398:404	arg1	model					511:515	lung fibrosis mouse model	491:515	lung fibrosis mouse model	491:515	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	5	5	dep	disaccharides	951:963	arg1	U0A6					966:969	U0A6	966:969	U0A6	966:969	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	0	6	theme	C57BL/6	89:95	arg1	model					103:107	the C57BL/6 mouse model	85:107	the C57BL/6 mouse model of lung fibrosis	85:124	A single injection of bleomycin reduces glycosaminoglycan sulfation up to 30 days in the C57BL/6 mouse model of lung fibrosis.
32422263	6	7	theme	HS	1112:1113	arg1	under-sulfation					1088:1102	under-sulfation	1088:1102	under-sulfation of both HS and CS disaccharides	1088:1134	At day 15, under-sulfation of both HS and CS disaccharides was persisted.
32422263	3	8	from	tissues	480:486	arg1	CS					436:437	CS	436:437	CS	436:437	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	3	8	from	tissues	480:486	arg1	HS					407:408	HS	407:408	HS	407:408	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	3	8	from	tissues	480:486	arg1	sulfate					398:404	heparan sulfate	390:404	heparan sulfate (HS)	390:409	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	3	8	from	tissues	480:486	arg1	sulfate					427:433	chondroitin sulfate	415:433	chondroitin sulfate (CS)	415:438	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	5	9	theme	CS	884:885	arg1	disaccharide					887:898	CS disaccharide	884:898	CS disaccharide (U0a2)	884:905	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	9	theme	CS	884:885	arg1	U0a2					901:904	U0a2	901:904	U0a2	901:904	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	8	10	theme	molecules	1503:1511	arg1	class					1474:1478	another class	1466:1478	another class of defective signaling molecules involved in bleomycin-induced lung fibrosis	1466:1555	In conclusion, GAGs, especially HS, from fibrotic lungs induced by a single injection of bleomycin were significantly under-sulfated up to 30 days, suggesting GAGs might be another class of defective signaling molecules involved in bleomycin-induced lung fibrosis.
32422263	1	11	theme	unknown	236:242	arg1	mechanism					244:252	unknown mechanism	236:252	unknown mechanism	236:252	Bleomycin is a clinically used anticancer drug, but it induces lung fibrosis in certain cancer patients with unknown mechanism.
32422263	3	12	theme	current	347:353	arg1	study					355:359	current study	347:359	current study	347:359	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	8	13	theme	fibrotic	1334:1341	arg1	lungs					1343:1347	fibrotic lungs	1334:1347	fibrotic lungs induced by a single injection of bleomycin	1334:1390	In conclusion, GAGs, especially HS, from fibrotic lungs induced by a single injection of bleomycin were significantly under-sulfated up to 30 days, suggesting GAGs might be another class of defective signaling molecules involved in bleomycin-induced lung fibrosis.
32422263	5	14	theme	disaccharide	887:898	arg1	disaccharide					919:930	hyaluranan disaccharide	908:930	hyaluranan disaccharide (UβA0)	908:937	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	14	theme	disaccharide	887:898	arg1	disaccharide					887:898	CS disaccharide	884:898	CS disaccharide (U0a2)	884:905	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	14	theme	disaccharide	887:898	arg1	disaccharides					951:963	six HS disaccharides	944:963	six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6)	944:1004	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	14	theme	disaccharide	887:898	arg1	U0a2					901:904	U0a2	901:904	U0a2	901:904	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	14	theme	disaccharide	887:898	arg1	UβA0					933:936	UβA0	933:936	UβA0	933:936	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	14	theme	disaccharide	887:898	arg1	amount					874:879	the amount	870:879	the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6)	870:1004	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	8	15	theme	bleomycin-induced	1525:1541	arg1	fibrosis					1548:1555	bleomycin-induced lung fibrosis	1525:1555	bleomycin-induced lung fibrosis	1525:1555	In conclusion, GAGs, especially HS, from fibrotic lungs induced by a single injection of bleomycin were significantly under-sulfated up to 30 days, suggesting GAGs might be another class of defective signaling molecules involved in bleomycin-induced lung fibrosis.
32422263	3	16	theme	bleomycin-induced	445:461	arg1	tissues					480:486	bleomycin-induced and control lung tissues	445:486	tissues	480:486	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	5	17	theme	disaccharides	720:732	arg1	U0A0					791:794	U0A0	791:794	U0A0	791:794	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	17	theme	disaccharides	720:732	arg1	amount					707:712	the amount	703:712	the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0)	703:795	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	17	theme	disaccharides	720:732	arg1	U0a6					741:744	U0a6	741:744	U0a6	741:744	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	17	theme	disaccharides	720:732	arg1	disaccharide					777:788	non-sulfated HS disaccharide	761:788	non-sulfated HS disaccharide (U0A0)	761:795	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	17	theme	disaccharides	720:732	arg1	disaccharides					720:732	CS disaccharides	717:732	CS disaccharides (U0a0, U0a6, and U0a4)	717:755	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	17	theme	disaccharides	720:732	arg1	U0a4					751:754	U0a4	751:754	U0a4	751:754	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	18	theme	non-sulfated	761:772	arg1	U0A0					791:794	U0A0	791:794	U0A0	791:794	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	18	theme	non-sulfated	761:772	arg1	disaccharide					777:788	non-sulfated HS disaccharide	761:788	non-sulfated HS disaccharide (U0A0)	761:795	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	3	19	theme	fibrosis	496:503	arg1	model					511:515	lung fibrosis mouse model	491:515	lung fibrosis mouse model	491:515	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	1	20	theme	used	153:156	arg1	drug					169:172	a clinically used anticancer drug	140:172	a clinically used anticancer drug	140:172	Bleomycin is a clinically used anticancer drug, but it induces lung fibrosis in certain cancer patients with unknown mechanism.
32422263	1	20	theme	used	153:156	arg1	Bleomycin					127:135	Bleomycin	127:135	Bleomycin	127:135	Bleomycin is a clinically used anticancer drug, but it induces lung fibrosis in certain cancer patients with unknown mechanism.
32422263	0	21	theme	mouse	97:101	arg1	model					103:107	the C57BL/6 mouse model	85:107	the C57BL/6 mouse model of lung fibrosis	85:124	A single injection of bleomycin reduces glycosaminoglycan sulfation up to 30 days in the C57BL/6 mouse model of lung fibrosis.
32422263	8	22	from	lungs	1343:1347	arg1	HS					1325:1326	HS	1325:1326	especially HS	1314:1326	In conclusion, GAGs, especially HS, from fibrotic lungs induced by a single injection of bleomycin were significantly under-sulfated up to 30 days, suggesting GAGs might be another class of defective signaling molecules involved in bleomycin-induced lung fibrosis.
32422263	8	22	from	lungs	1343:1347	arg1	GAGs					1308:1311	GAGs	1308:1311	GAGs	1308:1311	In conclusion, GAGs, especially HS, from fibrotic lungs induced by a single injection of bleomycin were significantly under-sulfated up to 30 days, suggesting GAGs might be another class of defective signaling molecules involved in bleomycin-induced lung fibrosis.
32422263	3	23	theme	mouse	505:509	arg1	model					511:515	lung fibrosis mouse model	491:515	lung fibrosis mouse model	491:515	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	1	24	theme	anticancer	158:167	arg1	drug					169:172	a clinically used anticancer drug	140:172	a clinically used anticancer drug	140:172	Bleomycin is a clinically used anticancer drug, but it induces lung fibrosis in certain cancer patients with unknown mechanism.
32422263	1	24	theme	anticancer	158:167	arg1	Bleomycin					127:135	Bleomycin	127:135	Bleomycin	127:135	Bleomycin is a clinically used anticancer drug, but it induces lung fibrosis in certain cancer patients with unknown mechanism.
32422263	0	25	theme	single	2:7	arg1	injection					9:17	A single injection	0:17	A single injection of bleomycin	0:30	A single injection of bleomycin reduces glycosaminoglycan sulfation up to 30 days in the C57BL/6 mouse model of lung fibrosis.
32422263	7	26	theme	control	1225:1231	arg1	levels					1233:1238	the control levels	1221:1238	the control levels	1221:1238	At day 30, the CS disaccharide compositions were recovered to that of the control levels whereas the HS were still remarkably under-sulfated.
32422263	5	27	dep	U0A6	966:969	arg1	U2S0					990:993	U2S0	990:993	U2S0	990:993	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	27	dep	U0A6	966:969	arg1	U2S6					1000:1003	U2S6	1000:1003	U2S6	1000:1003	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	27	dep	U0A6	966:969	arg1	U0H6					978:981	U0H6	978:981	U0H6	978:981	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	28	theme	HS	774:775	arg1	U0A0					791:794	U0A0	791:794	U0A0	791:794	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	28	theme	HS	774:775	arg1	disaccharide					777:788	non-sulfated HS disaccharide	761:788	non-sulfated HS disaccharide (U0A0)	761:795	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	29	theme	disaccharides	951:963	arg1	disaccharide					919:930	hyaluranan disaccharide	908:930	hyaluranan disaccharide (UβA0)	908:937	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	29	theme	disaccharides	951:963	arg1	disaccharide					887:898	CS disaccharide	884:898	CS disaccharide (U0a2)	884:905	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	29	theme	disaccharides	951:963	arg1	disaccharides					951:963	six HS disaccharides	944:963	six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6)	944:1004	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	29	theme	disaccharides	951:963	arg1	U0a2					901:904	U0a2	901:904	U0a2	901:904	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	29	theme	disaccharides	951:963	arg1	UβA0					933:936	UβA0	933:936	UβA0	933:936	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	29	theme	disaccharides	951:963	arg1	amount					874:879	the amount	870:879	the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6)	870:1004	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	0	30	dep	30 days	74:80	arg1	up					68:69	up	68:69	up	68:69	A single injection of bleomycin reduces glycosaminoglycan sulfation up to 30 days in the C57BL/6 mouse model of lung fibrosis.
32422263	0	31	theme	glycosaminoglycan	40:56	arg1	sulfation					58:66	glycosaminoglycan sulfation	40:66	glycosaminoglycan sulfation	40:66	A single injection of bleomycin reduces glycosaminoglycan sulfation up to 30 days in the C57BL/6 mouse model of lung fibrosis.
32422263	5	32	theme	disaccharide	777:788	arg1	U0A0					791:794	U0A0	791:794	U0A0	791:794	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	32	theme	disaccharide	777:788	arg1	amount					707:712	the amount	703:712	the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0)	703:795	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	32	theme	disaccharide	777:788	arg1	U0a6					741:744	U0a6	741:744	U0a6	741:744	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	32	theme	disaccharide	777:788	arg1	disaccharide					777:788	non-sulfated HS disaccharide	761:788	non-sulfated HS disaccharide (U0A0)	761:795	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	32	theme	disaccharide	777:788	arg1	disaccharides					720:732	CS disaccharides	717:732	CS disaccharides (U0a0, U0a6, and U0a4)	717:755	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	32	theme	disaccharide	777:788	arg1	U0a4					751:754	U0a4	751:754	U0a4	751:754	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	0	33	theme	lung	112:115	arg1	fibrosis					117:124	lung fibrosis	112:124	lung fibrosis	112:124	A single injection of bleomycin reduces glycosaminoglycan sulfation up to 30 days in the C57BL/6 mouse model of lung fibrosis.
32422263	8	34	dep	30 days	1432:1438	arg1	up					1426:1427	up	1426:1427	up	1426:1427	In conclusion, GAGs, especially HS, from fibrotic lungs induced by a single injection of bleomycin were significantly under-sulfated up to 30 days, suggesting GAGs might be another class of defective signaling molecules involved in bleomycin-induced lung fibrosis.
32422263	8	35	theme	lung	1543:1546	arg1	fibrosis					1548:1555	bleomycin-induced lung fibrosis	1525:1555	bleomycin-induced lung fibrosis	1525:1555	In conclusion, GAGs, especially HS, from fibrotic lungs induced by a single injection of bleomycin were significantly under-sulfated up to 30 days, suggesting GAGs might be another class of defective signaling molecules involved in bleomycin-induced lung fibrosis.
32422263	8	36	theme	single	1362:1367	arg1	injection					1369:1377	a single injection	1360:1377	a single injection of bleomycin	1360:1390	In conclusion, GAGs, especially HS, from fibrotic lungs induced by a single injection of bleomycin were significantly under-sulfated up to 30 days, suggesting GAGs might be another class of defective signaling molecules involved in bleomycin-induced lung fibrosis.
32422263	0	37	theme	bleomycin	22:30	arg1	injection					9:17	A single injection	0:17	A single injection of bleomycin	0:30	A single injection of bleomycin reduces glycosaminoglycan sulfation up to 30 days in the C57BL/6 mouse model of lung fibrosis.
32422263	8	38	theme	bleomycin	1382:1390	arg1	injection					1369:1377	a single injection	1360:1377	a single injection of bleomycin	1360:1390	In conclusion, GAGs, especially HS, from fibrotic lungs induced by a single injection of bleomycin were significantly under-sulfated up to 30 days, suggesting GAGs might be another class of defective signaling molecules involved in bleomycin-induced lung fibrosis.
32422263	0	39	theme	fibrosis	117:124	arg1	model					103:107	the C57BL/6 mouse model	85:107	the C57BL/6 mouse model of lung fibrosis	85:124	A single injection of bleomycin reduces glycosaminoglycan sulfation up to 30 days in the C57BL/6 mouse model of lung fibrosis.
32422263	5	40	theme	CS	717:718	arg1	disaccharides					720:732	CS disaccharides	717:732	CS disaccharides (U0a0, U0a6, and U0a4)	717:755	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	40	theme	CS	717:718	arg1	U0a6					741:744	U0a6	741:744	U0a6	741:744	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	40	theme	CS	717:718	arg1	U0a4					751:754	U0a4	751:754	U0a4	751:754	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	3	41	theme	deuterated	575:584	arg1	PMP					586:588	deuterated PMP	575:588	deuterated PMP	575:588	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	3	42	from	sulfate	427:433	arg1	model					511:515	lung fibrosis mouse model	491:515	lung fibrosis mouse model	491:515	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	1	43	theme	lung	190:193	arg1	fibrosis					195:202	lung fibrosis	190:202	lung fibrosis	190:202	Bleomycin is a clinically used anticancer drug, but it induces lung fibrosis in certain cancer patients with unknown mechanism.
32422263	6	44	dep	HS	1112:1113	arg1	disaccharides					1122:1134	disaccharides	1122:1134	disaccharides	1122:1134	At day 15, under-sulfation of both HS and CS disaccharides was persisted.
32422263	2	45	theme	animal	324:329	arg1	development					331:341	animal development	324:341	animal development	324:341	Glycosaminoglycans (GAGs) are required for lung morphogenesis during animal development.
32422263	3	46	theme	heparan	390:396	arg1	HS					407:408	HS	407:408	HS	407:408	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	3	46	theme	heparan	390:396	arg1	sulfate					398:404	heparan sulfate	390:404	heparan sulfate (HS)	390:409	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	1	47	with	patients	222:229	arg1	mechanism					244:252	unknown mechanism	236:252	unknown mechanism	236:252	Bleomycin is a clinically used anticancer drug, but it induces lung fibrosis in certain cancer patients with unknown mechanism.
32422263	3	48	theme	control	467:473	arg1	tissues					480:486	bleomycin-induced and control lung tissues	445:486	tissues	480:486	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	7	49	theme	CS	1166:1167	arg1	compositions					1182:1193	the CS disaccharide compositions	1162:1193	the CS disaccharide compositions	1162:1193	At day 30, the CS disaccharide compositions were recovered to that of the control levels whereas the HS were still remarkably under-sulfated.
32422263	7	50	theme	disaccharide	1169:1180	arg1	compositions					1182:1193	the CS disaccharide compositions	1162:1193	the CS disaccharide compositions	1162:1193	At day 30, the CS disaccharide compositions were recovered to that of the control levels whereas the HS were still remarkably under-sulfated.
32422263	5	51	theme	HS	948:949	arg1	disaccharides					951:963	six HS disaccharides	944:963	six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6)	944:1004	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	52	dep	increased	802:810	arg1	whereas					862:868	whereas	862:868	whereas	862:868	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	2	53	theme	lung	298:301	arg1	morphogenesis					303:315	lung morphogenesis	298:315	lung morphogenesis during animal development	298:341	Glycosaminoglycans (GAGs) are required for lung morphogenesis during animal development.
32422263	4	54	theme	isotope-tagged	624:637	arg1	disaccharides					639:651	The differentially isotope-tagged disaccharides	605:651	The differentially isotope-tagged disaccharides	605:651	The differentially isotope-tagged disaccharides were quantitatively compared by LC-MS.
32422263	5	55	theme	hyaluranan	908:917	arg1	UβA0					933:936	UβA0	933:936	UβA0	933:936	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	55	theme	hyaluranan	908:917	arg1	disaccharide					919:930	hyaluranan disaccharide	908:930	hyaluranan disaccharide (UβA0)	908:937	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	3	56	theme	chondroitin	415:425	arg1	CS					436:437	CS	436:437	CS	436:437	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	3	56	theme	chondroitin	415:425	arg1	sulfate					427:433	chondroitin sulfate	415:433	chondroitin sulfate (CS)	415:438	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	1	57	theme	certain	207:213	arg1	patients					222:229	certain cancer patients	207:229	certain cancer patients with unknown mechanism	207:252	Bleomycin is a clinically used anticancer drug, but it induces lung fibrosis in certain cancer patients with unknown mechanism.
32422263	5	58	theme	disaccharide	919:930	arg1	disaccharide					919:930	hyaluranan disaccharide	908:930	hyaluranan disaccharide (UβA0)	908:937	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	58	theme	disaccharide	919:930	arg1	disaccharide					887:898	CS disaccharide	884:898	CS disaccharide (U0a2)	884:905	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	58	theme	disaccharide	919:930	arg1	disaccharides					951:963	six HS disaccharides	944:963	six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6)	944:1004	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	58	theme	disaccharide	919:930	arg1	U0a2					901:904	U0a2	901:904	U0a2	901:904	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	58	theme	disaccharide	919:930	arg1	UβA0					933:936	UβA0	933:936	UβA0	933:936	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	5	58	theme	disaccharide	919:930	arg1	amount					874:879	the amount	870:879	the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6)	870:1004	At day 10, the amount of CS disaccharides (U0a0, U0a6, and U0a4) and non-sulfated HS disaccharide (U0A0) were increased by 1.3-, 1.6-, 11.7-, and 2.2-fold, respectively, whereas the amount of CS disaccharide (U0a2), hyaluranan disaccharide (UβA0), and six HS disaccharides (U0A6, U2A0, U0H6, U0S0, U2S0, and U2S6) were decreased from1.1- to 14.3-fold compared to that of the controls.
32422263	3	59	theme	lung	491:494	arg1	model					511:515	lung fibrosis mouse model	491:515	lung fibrosis mouse model	491:515	In current study, GAG disaccharides including heparan sulfate (HS) and chondroitin sulfate (CS) from bleomycin-induced and control lung tissues in lung fibrosis mouse model were tagged with 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated PMP, respectively.
32422263	8	60	theme	signaling	1493:1501	arg1	molecules					1503:1511	defective signaling molecules	1483:1511	defective signaling molecules involved in bleomycin-induced lung fibrosis	1483:1555	In conclusion, GAGs, especially HS, from fibrotic lungs induced by a single injection of bleomycin were significantly under-sulfated up to 30 days, suggesting GAGs might be another class of defective signaling molecules involved in bleomycin-induced lung fibrosis.
32422263	1	61	theme	cancer	215:220	arg1	patients					222:229	certain cancer patients	207:229	certain cancer patients with unknown mechanism	207:252	Bleomycin is a clinically used anticancer drug, but it induces lung fibrosis in certain cancer patients with unknown mechanism.
32541028	5	0	gly	Tn-OTS8-glycopeptide	971:990	arg2	Tn-OTS8-glycopeptide					971:990	the 237 Fab:Tn-OTS8-glycopeptide complex	959:998	the 237 Fab:Tn-OTS8-glycopeptide complex	959:998	Guided by the structure of the 237 Fab:Tn-OTS8-glycopeptide complex, here we conducted a deep mutational scan showing that residues flanking the Tn-glycan contributed significant binding energy to the interaction.
32541028	7	1	gly	Tn-glycoproteins	1388:1403	arg1	Tn-glycoproteins					1388:1403	multiple Tn-glycoproteins	1379:1403	multiple Tn-glycoproteins	1379:1403	Selection with a noncognate human antigen, Tn-MUC1, yielded scFv variants that were broadly reactive with multiple Tn-glycoproteins.
32541028	4	2	theme	mouse	835:839	arg1	cells					847:851	mouse tumor cells	835:851	mouse tumor cells	835:851	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	7	3	theme	multiple	1379:1386	arg1	Tn-glycoproteins					1388:1403	multiple Tn-glycoproteins	1379:1403	multiple Tn-glycoproteins	1379:1403	Selection with a noncognate human antigen, Tn-MUC1, yielded scFv variants that were broadly reactive with multiple Tn-glycoproteins.
32541028	3	4	contain	containing	575:584	arg1	proteins					566:573	cell surface proteins	553:573	cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate	553:715	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	3	4	contain	containing	575:584	arg2	O-glycans					586:594	O-glycans	586:594	O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate	586:715	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	3	5	theme	extended	695:702	arg1	carbohydrate					704:715	the normally extended carbohydrate	682:715	a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate	609:715	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	9	6	from	efficacy	1744:1751	arg1	mice					1735:1738	mice	1735:1738	mice	1735:1738	This strategy provides CARs with Tn-peptide specificities, all based on a single scFv scaffold, that allows the same CAR to be tested for toxicity in mice and efficacy against mouse and human tumors.
32541028	4	7	theme	cognate	867:873	arg1	epitope					891:897	its cognate Tn-glycopeptide epitope	863:897	its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8	863:929	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	8	8	from	glycosylation	1570:1582	arg1	defective					1548:1556	defective	1548:1556	defective	1548:1556	When configured as CARs, engineered T cells expressing these scFv variants showed improved activity against mouse and human cancer cell lines defective in O-linked glycosylation.
32541028	4	9	from	epitope	891:897	arg1	podoplanin					902:911	podoplanin	902:911	podoplanin	902:911	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	1	10	theme	hematopoietic	201:213	arg1	cancers					215:221	hematopoietic cancers	201:221	hematopoietic cancers	201:221	The potency of adoptive T cell therapies targeting the cell surface antigen CD19 has been demonstrated in hematopoietic cancers.
32541028	6	11	theme	display	1188:1194	arg1	system					1196:1201	the yeast display system	1178:1201	the yeast display system	1178:1201	Design of 237-scFv libraries in the yeast display system allowed us to isolate scFv variants with higher affinity for Tn-OTS8.
32541028	4	12	theme	cells	847:851	arg1	recognition					820:830	recognition	820:830	recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8	820:929	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	8	13	link	O-linked	1561:1568	arg1	glycosylation					1570:1582	O-linked glycosylation	1561:1582	O-linked glycosylation	1561:1582	When configured as CARs, engineered T cells expressing these scFv variants showed improved activity against mouse and human cancer cell lines defective in O-linked glycosylation.
32541028	7	14	with	Selection	1273:1281	arg1	antigen					1307:1313	a noncognate human antigen	1288:1313	a noncognate human antigen	1288:1313	Selection with a noncognate human antigen, Tn-MUC1, yielded scFv variants that were broadly reactive with multiple Tn-glycoproteins.
32541028	7	14	with	Selection	1273:1281	arg1	Tn-MUC1					1316:1322	Tn-MUC1	1316:1322	Tn-MUC1	1316:1322	Selection with a noncognate human antigen, Tn-MUC1, yielded scFv variants that were broadly reactive with multiple Tn-glycoproteins.
32541028	5	15	theme	Tn-OTS8-glycopeptide	971:990	arg1	complex					992:998	the 237 Fab:Tn-OTS8-glycopeptide complex	959:998	the 237 Fab:Tn-OTS8-glycopeptide complex	959:998	Guided by the structure of the 237 Fab:Tn-OTS8-glycopeptide complex, here we conducted a deep mutational scan showing that residues flanking the Tn-glycan contributed significant binding energy to the interaction.
32541028	3	16	theme	surface	558:564	arg1	proteins					566:573	cell surface proteins	553:573	cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate	553:715	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	1	17	theme	cell	121:124	arg1	therapies					126:134	adoptive T cell therapies	110:134	adoptive T cell therapies targeting the cell surface antigen CD19	110:174	The potency of adoptive T cell therapies targeting the cell surface antigen CD19 has been demonstrated in hematopoietic cancers.
32541028	6	18	theme	libraries	1165:1173	arg1	Design					1146:1151	Design	1146:1151	Design of 237-scFv libraries in the yeast display system	1146:1201	Design of 237-scFv libraries in the yeast display system allowed us to isolate scFv variants with higher affinity for Tn-OTS8.
32541028	6	19	theme	higher	1244:1249	arg1	affinity					1251:1258	higher affinity	1244:1258	higher affinity for Tn-OTS8	1244:1270	Design of 237-scFv libraries in the yeast display system allowed us to isolate scFv variants with higher affinity for Tn-OTS8.
32541028	2	20	theme	appropriate	258:268	arg1	targets					270:276	appropriate targets	258:276	appropriate targets in nonhematopoietic tumors	258:303	It has been difficult to identify appropriate targets in nonhematopoietic tumors, but one class of antigens that have shown promise is aberrant O-glycoprotein epitopes.
32541028	1	21	theme	therapies	126:134	arg1	potency					99:105	The potency	95:105	The potency of adoptive T cell therapies targeting the cell surface antigen CD19	95:174	The potency of adoptive T cell therapies targeting the cell surface antigen CD19 has been demonstrated in hematopoietic cancers.
32541028	8	22	theme	engineered	1431:1440	arg1	cells					1444:1448	engineered T cells	1431:1448	engineered T cells expressing these scFv variants	1431:1479	When configured as CARs, engineered T cells expressing these scFv variants showed improved activity against mouse and human cancer cell lines defective in O-linked glycosylation.
32541028	7	23	theme	noncognate	1290:1299	arg1	antigen					1307:1313	a noncognate human antigen	1288:1313	a noncognate human antigen	1288:1313	Selection with a noncognate human antigen, Tn-MUC1, yielded scFv variants that were broadly reactive with multiple Tn-glycoproteins.
32541028	7	23	theme	noncognate	1290:1299	arg1	Tn-MUC1					1316:1322	Tn-MUC1	1316:1322	Tn-MUC1	1316:1322	Selection with a noncognate human antigen, Tn-MUC1, yielded scFv variants that were broadly reactive with multiple Tn-glycoproteins.
32541028	0	24	theme	CARs	64:67	arg1	specificity					49:59	specificity	49:59	specificity	49:59	Structure-guided engineering of the affinity and specificity of CARs against Tn-glycopeptides.
32541028	0	24	theme	CARs	64:67	arg1	affinity					36:43	affinity	36:43	affinity	36:43	Structure-guided engineering of the affinity and specificity of CARs against Tn-glycopeptides.
32541028	1	25	theme	cell	150:153	arg1	CD19					171:174	the cell surface antigen CD19	146:174	the cell surface antigen CD19	146:174	The potency of adoptive T cell therapies targeting the cell surface antigen CD19 has been demonstrated in hematopoietic cancers.
32541028	6	26	from	Design	1146:1151	arg1	system					1196:1201	the yeast display system	1178:1201	the yeast display system	1178:1201	Design of 237-scFv libraries in the yeast display system allowed us to isolate scFv variants with higher affinity for Tn-OTS8.
32541028	1	27	theme	antigen	163:169	arg1	CD19					171:174	the cell surface antigen CD19	146:174	the cell surface antigen CD19	146:174	The potency of adoptive T cell therapies targeting the cell surface antigen CD19 has been demonstrated in hematopoietic cancers.
32541028	3	28	dep	sugars	478:483	arg1	threonine					457:465	threonine	457:465	threonine	457:465	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	3	28	dep	sugars	478:483	arg1	sugars					478:483	O-linked (threonine or serine) sugars	447:483	O-linked (threonine or serine) sugars	447:483	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	3	28	dep	sugars	478:483	arg1	serine					470:475	serine	470:475	serine	470:475	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	8	29	theme	improved	1488:1495	arg1	activity					1497:1504	improved activity	1488:1504	improved activity against mouse and human cancer cell lines defective in O-linked glycosylation	1488:1582	When configured as CARs, engineered T cells expressing these scFv variants showed improved activity against mouse and human cancer cell lines defective in O-linked glycosylation.
32541028	3	30	theme	dysregulated	421:432	arg1	synthesis					434:442	dysregulated synthesis	421:442	dysregulated synthesis of O-linked (threonine or serine) sugars	421:483	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	0	31	theme	Structure-guided	0:15	arg1	engineering					17:27	Structure-guided engineering	0:27	Structure-guided engineering of the affinity and specificity of CARs against Tn-glycopeptides	0:92	Structure-guided engineering of the affinity and specificity of CARs against Tn-glycopeptides.
32541028	1	32	theme	adoptive	110:117	arg1	therapies					126:134	adoptive T cell therapies	110:134	adoptive T cell therapies targeting the cell surface antigen CD19	110:174	The potency of adoptive T cell therapies targeting the cell surface antigen CD19 has been demonstrated in hematopoietic cancers.
32541028	2	33	from	targets	270:276	arg1	tumors					298:303	nonhematopoietic tumors	281:303	nonhematopoietic tumors	281:303	It has been difficult to identify appropriate targets in nonhematopoietic tumors, but one class of antigens that have shown promise is aberrant O-glycoprotein epitopes.
32541028	4	34	theme	chimeric	777:784	arg1	fragment					747:754	the scFv fragment	738:754	the scFv fragment of antibody 237	738:770	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	4	34	theme	chimeric	777:784	arg1	CAR					804:806	CAR	804:806	CAR	804:806	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	4	34	theme	chimeric	777:784	arg1	receptor					794:801	a chimeric antigen receptor	775:801	a chimeric antigen receptor (CAR)	775:807	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	0	35	theme	affinity	36:43	arg1	engineering					17:27	Structure-guided engineering	0:27	Structure-guided engineering of the affinity and specificity of CARs against Tn-glycopeptides	0:92	Structure-guided engineering of the affinity and specificity of CARs against Tn-glycopeptides.
32541028	8	36	theme	cancer	1530:1535	arg1	lines					1542:1546	mouse and human cancer cell lines	1514:1546	lines	1542:1546	When configured as CARs, engineered T cells expressing these scFv variants showed improved activity against mouse and human cancer cell lines defective in O-linked glycosylation.
32541028	0	37	theme	specificity	49:59	arg1	engineering					17:27	Structure-guided engineering	0:27	Structure-guided engineering of the affinity and specificity of CARs against Tn-glycopeptides	0:92	Structure-guided engineering of the affinity and specificity of CARs against Tn-glycopeptides.
32541028	5	38	theme	binding	1111:1117	arg1	energy					1119:1124	significant binding energy	1099:1124	significant binding energy	1099:1124	Guided by the structure of the 237 Fab:Tn-OTS8-glycopeptide complex, here we conducted a deep mutational scan showing that residues flanking the Tn-glycan contributed significant binding energy to the interaction.
32541028	0	39	dep	affinity	36:43	arg1	the					32:34	the	32:34	the	32:34	Structure-guided engineering of the affinity and specificity of CARs against Tn-glycopeptides.
32541028	3	40	theme	Tn	658:659	arg1	antigen					661:667	Tn antigen	658:667	Tn antigen	658:667	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	4	41	theme	antibody	759:766	arg1	237					768:770	antibody 237	759:770	antibody 237	759:770	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	2	42	theme	O-glycoprotein	368:381	arg1	epitopes					383:390	aberrant O-glycoprotein epitopes	359:390	aberrant O-glycoprotein epitopes	359:390	It has been difficult to identify appropriate targets in nonhematopoietic tumors, but one class of antigens that have shown promise is aberrant O-glycoprotein epitopes.
32541028	3	43	theme	many	495:498	arg1	cancers					500:506	many cancers	495:506	many cancers	495:506	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	9	44	theme	Tn-peptide	1618:1627	arg1	specificities					1629:1641	Tn-peptide specificities	1618:1641	Tn-peptide specificities	1618:1641	This strategy provides CARs with Tn-peptide specificities, all based on a single scFv scaffold, that allows the same CAR to be tested for toxicity in mice and efficacy against mouse and human tumors.
32541028	9	44	theme	Tn-peptide	1618:1627	arg1	all					1644:1646	all	1644:1646	all	1644:1646	This strategy provides CARs with Tn-peptide specificities, all based on a single scFv scaffold, that allows the same CAR to be tested for toxicity in mice and efficacy against mouse and human tumors.
32541028	6	45	theme	scFv	1225:1228	arg1	variants					1230:1237	scFv variants	1225:1237	scFv variants	1225:1237	Design of 237-scFv libraries in the yeast display system allowed us to isolate scFv variants with higher affinity for Tn-OTS8.
32541028	8	46	from	defective	1548:1556	arg1	glycosylation					1570:1582	O-linked glycosylation	1561:1582	O-linked glycosylation	1561:1582	When configured as CARs, engineered T cells expressing these scFv variants showed improved activity against mouse and human cancer cell lines defective in O-linked glycosylation.
32541028	2	47	theme	nonhematopoietic	281:296	arg1	tumors					298:303	nonhematopoietic tumors	281:303	nonhematopoietic tumors	281:303	It has been difficult to identify appropriate targets in nonhematopoietic tumors, but one class of antigens that have shown promise is aberrant O-glycoprotein epitopes.
32541028	8	48	theme	defective	1548:1556	arg1	lines					1542:1546	mouse and human cancer cell lines	1514:1546	lines	1542:1546	When configured as CARs, engineered T cells expressing these scFv variants showed improved activity against mouse and human cancer cell lines defective in O-linked glycosylation.
32541028	3	49	link	O-linked	447:454	arg1	threonine					457:465	threonine	457:465	threonine	457:465	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	3	49	link	O-linked	447:454	arg1	sugars					478:483	O-linked (threonine or serine) sugars	447:483	O-linked (threonine or serine) sugars	447:483	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	3	49	link	O-linked	447:454	arg1	serine					470:475	serine	470:475	serine	470:475	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	9	50	theme	scFv	1666:1669	arg1	scaffold					1671:1678	a single scFv scaffold	1657:1678	a single scFv scaffold	1657:1678	This strategy provides CARs with Tn-peptide specificities, all based on a single scFv scaffold, that allows the same CAR to be tested for toxicity in mice and efficacy against mouse and human tumors.
32541028	3	51	theme	proteins	566:573	arg1	expression					539:548	the expression	535:548	the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate	535:715	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	4	52	theme	Tn-glycopeptide	875:889	arg1	epitope					891:897	its cognate Tn-glycopeptide epitope	863:897	its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8	863:929	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	6	53	theme	yeast	1182:1186	arg1	system					1196:1201	the yeast display system	1178:1201	the yeast display system	1178:1201	Design of 237-scFv libraries in the yeast display system allowed us to isolate scFv variants with higher affinity for Tn-OTS8.
32541028	3	54	theme	cell	553:556	arg1	proteins					566:573	cell surface proteins	553:573	cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate	553:715	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	1	55	theme	T	119:119	arg1	therapies					126:134	adoptive T cell therapies	110:134	adoptive T cell therapies targeting the cell surface antigen CD19	110:174	The potency of adoptive T cell therapies targeting the cell surface antigen CD19 has been demonstrated in hematopoietic cancers.
32541028	4	56	theme	tumor	841:845	arg1	cells					847:851	mouse tumor cells	835:851	mouse tumor cells	835:851	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	5	57	theme	complex	992:998	arg1	structure					946:954	the structure	942:954	the structure of the 237 Fab:Tn-OTS8-glycopeptide complex	942:998	Guided by the structure of the 237 Fab:Tn-OTS8-glycopeptide complex, here we conducted a deep mutational scan showing that residues flanking the Tn-glycan contributed significant binding energy to the interaction.
32541028	4	58	gly	Tn-glycopeptide	875:889	arg2	Tn-glycopeptide					875:889	its cognate Tn-glycopeptide epitope	863:897	its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8	863:929	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	9	59	theme	same	1697:1700	arg1	CAR					1702:1704	the same CAR	1693:1704	the same CAR	1693:1704	This strategy provides CARs with Tn-peptide specificities, all based on a single scFv scaffold, that allows the same CAR to be tested for toxicity in mice and efficacy against mouse and human tumors.
32541028	8	60	theme	T	1442:1442	arg1	cells					1444:1448	engineered T cells	1431:1448	engineered T cells expressing these scFv variants	1431:1479	When configured as CARs, engineered T cells expressing these scFv variants showed improved activity against mouse and human cancer cell lines defective in O-linked glycosylation.
32541028	6	61	theme	237-scFv	1156:1163	arg1	libraries					1165:1173	237-scFv libraries	1156:1173	237-scFv libraries	1156:1173	Design of 237-scFv libraries in the yeast display system allowed us to isolate scFv variants with higher affinity for Tn-OTS8.
32541028	7	62	theme	human	1301:1305	arg1	antigen					1307:1313	a noncognate human antigen	1288:1313	a noncognate human antigen	1288:1313	Selection with a noncognate human antigen, Tn-MUC1, yielded scFv variants that were broadly reactive with multiple Tn-glycoproteins.
32541028	7	62	theme	human	1301:1305	arg1	Tn-MUC1					1316:1322	Tn-MUC1	1316:1322	Tn-MUC1	1316:1322	Selection with a noncognate human antigen, Tn-MUC1, yielded scFv variants that were broadly reactive with multiple Tn-glycoproteins.
32541028	1	63	theme	surface	155:161	arg1	CD19					171:174	the cell surface antigen CD19	146:174	the cell surface antigen CD19	146:174	The potency of adoptive T cell therapies targeting the cell surface antigen CD19 has been demonstrated in hematopoietic cancers.
32541028	8	64	theme	scFv	1467:1470	arg1	variants					1472:1479	these scFv variants	1461:1479	these scFv variants	1461:1479	When configured as CARs, engineered T cells expressing these scFv variants showed improved activity against mouse and human cancer cell lines defective in O-linked glycosylation.
32541028	2	65	theme	antigens	323:330	arg1	class					314:318	one class	310:318	one class of antigens that have shown promise	310:354	It has been difficult to identify appropriate targets in nonhematopoietic tumors, but one class of antigens that have shown promise is aberrant O-glycoprotein epitopes.
32541028	0	66	gly	Tn-glycopeptides	77:92	arg2	Tn-glycopeptides					77:92	Tn-glycopeptides	77:92	Tn-glycopeptides	77:92	Structure-guided engineering of the affinity and specificity of CARs against Tn-glycopeptides.
32541028	4	67	contain	bear	858:861	arg2	epitope					891:897	its cognate Tn-glycopeptide epitope	863:897	its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8	863:929	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	4	67	contain	bear	858:861	arg1	recognition					820:830	recognition	820:830	recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8	820:929	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	4	68	theme	scFv	742:745	arg1	receptor					794:801	a chimeric antigen receptor	775:801	a chimeric antigen receptor (CAR)	775:807	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	4	68	theme	scFv	742:745	arg1	fragment					747:754	the scFv fragment	738:754	the scFv fragment of antibody 237	738:770	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	3	69	theme	O-linked	447:454	arg1	threonine					457:465	threonine	457:465	threonine	457:465	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	3	69	theme	O-linked	447:454	arg1	sugars					478:483	O-linked (threonine or serine) sugars	447:483	O-linked (threonine or serine) sugars	447:483	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	3	69	theme	O-linked	447:454	arg1	serine					470:475	serine	470:475	serine	470:475	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	8	70	theme	mouse	1514:1518	arg1	lines					1542:1546	mouse and human cancer cell lines	1514:1546	lines	1542:1546	When configured as CARs, engineered T cells expressing these scFv variants showed improved activity against mouse and human cancer cell lines defective in O-linked glycosylation.
32541028	9	71	theme	mouse	1761:1765	arg1	tumors					1777:1782	mouse and human tumors	1761:1782	mouse and human tumors	1761:1782	This strategy provides CARs with Tn-peptide specificities, all based on a single scFv scaffold, that allows the same CAR to be tested for toxicity in mice and efficacy against mouse and human tumors.
32541028	8	72	theme	human	1524:1528	arg1	lines					1542:1546	mouse and human cancer cell lines	1514:1546	lines	1542:1546	When configured as CARs, engineered T cells expressing these scFv variants showed improved activity against mouse and human cancer cell lines defective in O-linked glycosylation.
32541028	2	73	gly	O-glycoprotein	368:381	arg1	O-glycoprotein					368:381	aberrant O-glycoprotein epitopes	359:390	aberrant O-glycoprotein epitopes	359:390	It has been difficult to identify appropriate targets in nonhematopoietic tumors, but one class of antigens that have shown promise is aberrant O-glycoprotein epitopes.
32541028	7	74	with	reactive	1365:1372	arg1	Tn-glycoproteins					1388:1403	multiple Tn-glycoproteins	1379:1403	multiple Tn-glycoproteins	1379:1403	Selection with a noncognate human antigen, Tn-MUC1, yielded scFv variants that were broadly reactive with multiple Tn-glycoproteins.
32541028	9	75	theme	human	1771:1775	arg1	tumors					1777:1782	mouse and human tumors	1761:1782	mouse and human tumors	1761:1782	This strategy provides CARs with Tn-peptide specificities, all based on a single scFv scaffold, that allows the same CAR to be tested for toxicity in mice and efficacy against mouse and human tumors.
32541028	8	76	theme	cell	1537:1540	arg1	lines					1542:1546	mouse and human cancer cell lines	1514:1546	lines	1542:1546	When configured as CARs, engineered T cells expressing these scFv variants showed improved activity against mouse and human cancer cell lines defective in O-linked glycosylation.
32541028	3	77	theme	single	611:616	arg1	N-acetylgalactosamine					618:638	a single N-acetylgalactosamine	609:638	a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate	609:715	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	5	78	theme	significant	1099:1109	arg1	energy					1119:1124	significant binding energy	1099:1124	significant binding energy	1099:1124	Guided by the structure of the 237 Fab:Tn-OTS8-glycopeptide complex, here we conducted a deep mutational scan showing that residues flanking the Tn-glycan contributed significant binding energy to the interaction.
32541028	9	79	from	toxicity	1723:1730	arg1	mice					1735:1738	mice	1735:1738	mice	1735:1738	This strategy provides CARs with Tn-peptide specificities, all based on a single scFv scaffold, that allows the same CAR to be tested for toxicity in mice and efficacy against mouse and human tumors.
32541028	5	80	theme	Fab	967:969	arg1	complex					992:998	the 237 Fab:Tn-OTS8-glycopeptide complex	959:998	the 237 Fab:Tn-OTS8-glycopeptide complex	959:998	Guided by the structure of the 237 Fab:Tn-OTS8-glycopeptide complex, here we conducted a deep mutational scan showing that residues flanking the Tn-glycan contributed significant binding energy to the interaction.
32541028	5	81	dep	mutational	1026:1035	arg1	scan					1037:1040	scan	1037:1040	scan	1037:1040	Guided by the structure of the 237 Fab:Tn-OTS8-glycopeptide complex, here we conducted a deep mutational scan showing that residues flanking the Tn-glycan contributed significant binding energy to the interaction.
32541028	4	82	theme	antigen	786:792	arg1	fragment					747:754	the scFv fragment	738:754	the scFv fragment of antibody 237	738:770	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	4	82	theme	antigen	786:792	arg1	CAR					804:806	CAR	804:806	CAR	804:806	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	4	82	theme	antigen	786:792	arg1	receptor					794:801	a chimeric antigen receptor	775:801	a chimeric antigen receptor (CAR)	775:807	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	9	83	theme	single	1659:1664	arg1	scaffold					1671:1678	a single scFv scaffold	1657:1678	a single scFv scaffold	1657:1678	This strategy provides CARs with Tn-peptide specificities, all based on a single scFv scaffold, that allows the same CAR to be tested for toxicity in mice and efficacy against mouse and human tumors.
32541028	7	84	theme	scFv	1333:1336	arg1	variants					1338:1345	scFv variants	1333:1345	scFv variants that were broadly reactive with multiple Tn-glycoproteins	1333:1403	Selection with a noncognate human antigen, Tn-MUC1, yielded scFv variants that were broadly reactive with multiple Tn-glycoproteins.
32541028	8	85	theme	O-linked	1561:1568	arg1	glycosylation					1570:1582	O-linked glycosylation	1561:1582	O-linked glycosylation	1561:1582	When configured as CARs, engineered T cells expressing these scFv variants showed improved activity against mouse and human cancer cell lines defective in O-linked glycosylation.
32541028	3	86	theme	sugars	478:483	arg1	synthesis					434:442	dysregulated synthesis	421:442	dysregulated synthesis of O-linked (threonine or serine) sugars	421:483	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	4	87	used	used	733:736	arg2	we					730:731	we	730:731	we	730:731	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	3	88	dep	N-acetylgalactosamine	618:638	arg1	GalNAc					641:646	GalNAc	641:646	GalNAc	641:646	It has long been known that dysregulated synthesis of O-linked (threonine or serine) sugars occurs in many cancers, and that this can lead to the expression of cell surface proteins containing O-glycans comprised of a single N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydrate.
32541028	9	89	with	CARs	1608:1611	arg1	specificities					1629:1641	Tn-peptide specificities	1618:1641	Tn-peptide specificities	1618:1641	This strategy provides CARs with Tn-peptide specificities, all based on a single scFv scaffold, that allows the same CAR to be tested for toxicity in mice and efficacy against mouse and human tumors.
32541028	9	89	with	CARs	1608:1611	arg1	all					1644:1646	all	1644:1646	all	1644:1646	This strategy provides CARs with Tn-peptide specificities, all based on a single scFv scaffold, that allows the same CAR to be tested for toxicity in mice and efficacy against mouse and human tumors.
32541028	4	90	theme	237	768:770	arg1	receptor					794:801	a chimeric antigen receptor	775:801	a chimeric antigen receptor (CAR)	775:807	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	4	90	theme	237	768:770	arg1	fragment					747:754	the scFv fragment	738:754	the scFv fragment of antibody 237	738:770	Previously, we used the scFv fragment of antibody 237 as a chimeric antigen receptor (CAR) to mediate recognition of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.
32541028	2	91	theme	aberrant	359:366	arg1	epitopes					383:390	aberrant O-glycoprotein epitopes	359:390	aberrant O-glycoprotein epitopes	359:390	It has been difficult to identify appropriate targets in nonhematopoietic tumors, but one class of antigens that have shown promise is aberrant O-glycoprotein epitopes.
33797224	2	0	theme	antiadhesion	497:508	arg1	agents					510:515	antiadhesion agents	497:515	antiadhesion agents	497:515	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	3	1	theme	solution	739:746	arg1	type					748:751	the solution type	735:751	the solution type (ST)	735:756	This article represents the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST).
33797224	7	2	theme	antiadhesion	1430:1441	arg1	agents					1443:1448	these antiadhesion agents	1424:1448	these antiadhesion agents	1424:1448	Thus, we believe these antiadhesion agents could be promising to reduce adhesion-related complications during and post-surgical operations and deserve consideration for further study for clinical purposes.
33797224	6	3	theme	ST	1320:1321	arg1	2.25-10					1323:1329	ST 2.25-10	1320:1329	ST 2.25-10	1320:1329	Medicurtain effectively prevented postlaparotomy adhesion in ∼42% of experimental animals, whereas ST 2.25-10, ST 2.5-5, ST 2.5-10, FT 20, and TST 1.5 were effective in 100% of animals.
33797224	1	4	theme	treatment	191:199	arg1	approaches					201:210	Pharmacological-based treatment approaches	169:210	Pharmacological-based treatment approaches	169:210	Pharmacological-based treatment approaches have been used over time to prevent postlaparotomy adhesion.
33797224	4	5	theme	novel	1043:1047	arg1	agents					1062:1067	novel antiadhesion agents	1043:1067	novel antiadhesion agents	1043:1067	These antiadhesion agents that contain no drugs exhibit excellent physical characteristics and superior stability over 30 days in the operative sites without any toxicity and side effects that make the compositions strong candidates as novel antiadhesion agents.
33797224	5	6	theme	tissue	1130:1135	arg1	properties					1150:1159	tissue regeneration properties	1130:1159	tissue regeneration properties	1130:1159	Also, the proposed samples reveal superior antiadhesion and tissue regeneration properties in Sprague-Dawley (SD) rats after surgery over Medicurtain.
33797224	5	7	theme	regeneration	1137:1148	arg1	properties					1150:1159	tissue regeneration properties	1130:1159	tissue regeneration properties	1130:1159	Also, the proposed samples reveal superior antiadhesion and tissue regeneration properties in Sprague-Dawley (SD) rats after surgery over Medicurtain.
33797224	6	8	theme	experimental	1290:1301	arg1	animals					1303:1309	experimental animals	1290:1309	experimental animals	1290:1309	Medicurtain effectively prevented postlaparotomy adhesion in ∼42% of experimental animals, whereas ST 2.25-10, ST 2.5-5, ST 2.5-10, FT 20, and TST 1.5 were effective in 100% of animals.
33797224	7	9	dep	operations	1535:1544	arg1	post-surgical					1521:1533	post-surgical	1521:1533	post-surgical	1521:1533	Thus, we believe these antiadhesion agents could be promising to reduce adhesion-related complications during and post-surgical operations and deserve consideration for further study for clinical purposes.
33797224	0	10	theme	Regeneration	111:122	arg1	Properties					124:133	Tissue Regeneration Properties	104:133	Tissue Regeneration Properties	104:133	Carboxymethyl Cellulose, Pluronic, and Pullulan-Based Compositions Efficiently Enhance Antiadhesion and Tissue Regeneration Properties without Using Any Drug Molecules.
33797224	4	11	theme	physical	873:880	arg1	characteristics					882:896	excellent physical characteristics	863:896	excellent physical characteristics	863:896	These antiadhesion agents that contain no drugs exhibit excellent physical characteristics and superior stability over 30 days in the operative sites without any toxicity and side effects that make the compositions strong candidates as novel antiadhesion agents.
33797224	3	12	theme	biocompatible	561:573	arg1	agents					606:611	biocompatible and biodegradable antiadhesion agents	561:611	biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST)	561:804	This article represents the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST).
33797224	0	13	theme	Tissue	104:109	arg1	Properties					124:133	Tissue Regeneration Properties	104:133	Tissue Regeneration Properties	104:133	Carboxymethyl Cellulose, Pluronic, and Pullulan-Based Compositions Efficiently Enhance Antiadhesion and Tissue Regeneration Properties without Using Any Drug Molecules.
33797224	4	14	theme	excellent	863:871	arg1	characteristics					882:896	excellent physical characteristics	863:896	excellent physical characteristics	863:896	These antiadhesion agents that contain no drugs exhibit excellent physical characteristics and superior stability over 30 days in the operative sites without any toxicity and side effects that make the compositions strong candidates as novel antiadhesion agents.
33797224	0	15	theme	Carboxymethyl	0:12	arg1	Cellulose					14:22	Carboxymethyl Cellulose	0:22	Carboxymethyl Cellulose	0:22	Carboxymethyl Cellulose, Pluronic, and Pullulan-Based Compositions Efficiently Enhance Antiadhesion and Tissue Regeneration Properties without Using Any Drug Molecules.
33797224	3	16	with	cellulose	643:651	arg1	types					693:697	three different types	677:697	three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST)	677:804	This article represents the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST).
33797224	5	17	theme	superior	1104:1111	arg1	antiadhesion					1113:1124	superior antiadhesion	1104:1124	superior antiadhesion	1104:1124	Also, the proposed samples reveal superior antiadhesion and tissue regeneration properties in Sprague-Dawley (SD) rats after surgery over Medicurtain.
33797224	2	18	theme	therapeutics	307:318	arg1	elimination					292:302	the rapid elimination	282:302	the rapid elimination of therapeutics from the peritoneum	282:338	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	2	18	theme	therapeutics	307:318	arg1	effects					365:371	their unwanted side effects	345:371	their unwanted side effects	345:371	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	2	18	theme	therapeutics	307:318	arg1	flow					379:382	easy flow	374:382	easy flow from the wound site by gravity	374:413	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	2	18	theme	therapeutics	307:318	arg1	efficacy					436:443	low therapeutic efficacy	420:443	low therapeutic efficacy	420:443	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	4	19	theme	strong	1022:1027	arg1	candidates					1029:1038	the compositions strong candidates	1005:1038	the compositions strong candidates as novel antiadhesion agents	1005:1067	These antiadhesion agents that contain no drugs exhibit excellent physical characteristics and superior stability over 30 days in the operative sites without any toxicity and side effects that make the compositions strong candidates as novel antiadhesion agents.
33797224	2	20	theme	unwanted	351:358	arg1	effects					365:371	their unwanted side effects	345:371	their unwanted side effects	345:371	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	2	21	theme	urgent	458:463	arg1	need					465:468	the urgent need	454:468	the urgent need for the next generation of antiadhesion agents	454:515	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	4	22	theme	compositions	1009:1020	arg1	candidates					1029:1038	the compositions strong candidates	1005:1038	the compositions strong candidates as novel antiadhesion agents	1005:1067	These antiadhesion agents that contain no drugs exhibit excellent physical characteristics and superior stability over 30 days in the operative sites without any toxicity and side effects that make the compositions strong candidates as novel antiadhesion agents.
33797224	3	23	with	pullulan	663:670	arg1	types					693:697	three different types	677:697	three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST)	677:804	This article represents the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST).
33797224	4	24	from	days	929:932	arg1	sites					951:955	the operative sites	937:955	the operative sites without any toxicity and side effects that make the compositions strong candidates as novel antiadhesion agents	937:1067	These antiadhesion agents that contain no drugs exhibit excellent physical characteristics and superior stability over 30 days in the operative sites without any toxicity and side effects that make the compositions strong candidates as novel antiadhesion agents.
33797224	6	25	theme	animals	1398:1404	arg1	animals					1398:1404	animals	1398:1404	animals	1398:1404	Medicurtain effectively prevented postlaparotomy adhesion in ∼42% of experimental animals, whereas ST 2.25-10, ST 2.5-5, ST 2.5-10, FT 20, and TST 1.5 were effective in 100% of animals.
33797224	6	25	theme	animals	1398:1404	arg1	%					1393:1393	100%	1390:1393	100% of animals	1390:1404	Medicurtain effectively prevented postlaparotomy adhesion in ∼42% of experimental animals, whereas ST 2.25-10, ST 2.5-5, ST 2.5-10, FT 20, and TST 1.5 were effective in 100% of animals.
33797224	3	26	theme	biodegradable	579:591	arg1	agents					606:611	biocompatible and biodegradable antiadhesion agents	561:611	biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST)	561:804	This article represents the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST).
33797224	5	27	from	properties	1150:1159	arg1	rats					1184:1187	Sprague-Dawley (SD) rats	1164:1187	Sprague-Dawley (SD) rats after surgery over Medicurtain	1164:1218	Also, the proposed samples reveal superior antiadhesion and tissue regeneration properties in Sprague-Dawley (SD) rats after surgery over Medicurtain.
33797224	3	28	theme	antiadhesion	593:604	arg1	agents					606:611	biocompatible and biodegradable antiadhesion agents	561:611	biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST)	561:804	This article represents the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST).
33797224	2	29	theme	therapeutic	424:434	arg1	efficacy					436:443	low therapeutic efficacy	420:443	low therapeutic efficacy	420:443	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	3	30	theme	film	759:762	arg1	type					764:767	film type	759:767	film type (FT)	759:772	This article represents the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST).
33797224	3	30	theme	film	759:762	arg1	FT					770:771	FT	770:771	FT	770:771	This article represents the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST).
33797224	3	31	theme	different	683:691	arg1	types					693:697	three different types	677:697	three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST)	677:804	This article represents the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST).
33797224	3	32	theme	carboxymethyl	629:641	arg1	CMC					654:656	CMC	654:656	CMC	654:656	This article represents the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST).
33797224	3	32	theme	carboxymethyl	629:641	arg1	cellulose					643:651	carboxymethyl cellulose	629:651	carboxymethyl cellulose (CMC)	629:657	This article represents the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST).
33797224	7	33	theme	adhesion-related	1479:1494	arg1	complications					1496:1508	adhesion-related complications	1479:1508	adhesion-related complications	1479:1508	Thus, we believe these antiadhesion agents could be promising to reduce adhesion-related complications during and post-surgical operations and deserve consideration for further study for clinical purposes.
33797224	2	34	theme	low	420:422	arg1	efficacy					436:443	low therapeutic efficacy	420:443	low therapeutic efficacy	420:443	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	2	35	theme	next	478:481	arg1	generation					483:492	the next generation	474:492	the next generation of antiadhesion agents	474:515	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	3	36	dep	type	748:751	arg1	ST					754:755	ST	754:755	ST	754:755	This article represents the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST).
33797224	0	37	theme	Drug	153:156	arg1	Molecules					158:166	Any Drug Molecules	149:166	Any Drug Molecules	149:166	Carboxymethyl Cellulose, Pluronic, and Pullulan-Based Compositions Efficiently Enhance Antiadhesion and Tissue Regeneration Properties without Using Any Drug Molecules.
33797224	6	38	theme	postlaparotomy	1255:1268	arg1	adhesion					1270:1277	postlaparotomy adhesion	1255:1277	postlaparotomy adhesion	1255:1277	Medicurtain effectively prevented postlaparotomy adhesion in ∼42% of experimental animals, whereas ST 2.25-10, ST 2.5-5, ST 2.5-10, FT 20, and TST 1.5 were effective in 100% of animals.
33797224	1	39	theme	postlaparotomy	248:261	arg1	adhesion					263:270	postlaparotomy adhesion	248:270	postlaparotomy adhesion	248:270	Pharmacological-based treatment approaches have been used over time to prevent postlaparotomy adhesion.
33797224	4	40	theme	operative	941:949	arg1	sites					951:955	the operative sites	937:955	the operative sites without any toxicity and side effects that make the compositions strong candidates as novel antiadhesion agents	937:1067	These antiadhesion agents that contain no drugs exhibit excellent physical characteristics and superior stability over 30 days in the operative sites without any toxicity and side effects that make the compositions strong candidates as novel antiadhesion agents.
33797224	5	41	theme	proposed	1080:1087	arg1	samples					1089:1095	the proposed samples	1076:1095	the proposed samples	1076:1095	Also, the proposed samples reveal superior antiadhesion and tissue regeneration properties in Sprague-Dawley (SD) rats after surgery over Medicurtain.
33797224	4	42	theme	antiadhesion	813:824	arg1	agents					826:831	These antiadhesion agents	807:831	These antiadhesion agents that contain no drugs	807:853	These antiadhesion agents that contain no drugs exhibit excellent physical characteristics and superior stability over 30 days in the operative sites without any toxicity and side effects that make the compositions strong candidates as novel antiadhesion agents.
33797224	2	43	from	site	399:402	arg1	elimination					292:302	the rapid elimination	282:302	the rapid elimination of therapeutics from the peritoneum	282:338	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	2	43	from	site	399:402	arg1	effects					365:371	their unwanted side effects	345:371	their unwanted side effects	345:371	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	2	43	from	site	399:402	arg1	flow					379:382	easy flow	374:382	easy flow from the wound site by gravity	374:413	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	2	43	from	site	399:402	arg1	efficacy					436:443	low therapeutic efficacy	420:443	low therapeutic efficacy	420:443	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	2	44	theme	easy	374:377	arg1	flow					379:382	easy flow	374:382	easy flow from the wound site by gravity	374:413	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	0	45	theme	Pullulan-Based	39:52	arg1	Compositions					54:65	Pullulan-Based Compositions	39:65	Pullulan-Based Compositions	39:65	Carboxymethyl Cellulose, Pluronic, and Pullulan-Based Compositions Efficiently Enhance Antiadhesion and Tissue Regeneration Properties without Using Any Drug Molecules.
33797224	7	46	theme	clinical	1594:1601	arg1	purposes					1603:1610	clinical purposes	1594:1610	clinical purposes	1594:1610	Thus, we believe these antiadhesion agents could be promising to reduce adhesion-related complications during and post-surgical operations and deserve consideration for further study for clinical purposes.
33797224	3	47	theme	physical	702:709	arg1	type					764:767	film type	759:767	film type (FT)	759:772	This article represents the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST).
33797224	3	47	theme	physical	702:709	arg1	type					795:798	thermosensitive type	779:798	thermosensitive type (TST)	779:804	This article represents the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST).
33797224	3	47	theme	physical	702:709	arg1	type					748:751	the solution type	735:751	the solution type (ST)	735:756	This article represents the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST).
33797224	3	47	theme	physical	702:709	arg1	characteristics					711:725	physical characteristics	702:725	physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST)	702:804	This article represents the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST).
33797224	6	48	from	effective	1377:1385	arg1	animals					1398:1404	animals	1398:1404	animals	1398:1404	Medicurtain effectively prevented postlaparotomy adhesion in ∼42% of experimental animals, whereas ST 2.25-10, ST 2.5-5, ST 2.5-10, FT 20, and TST 1.5 were effective in 100% of animals.
33797224	6	48	from	effective	1377:1385	arg1	%					1393:1393	100%	1390:1393	100% of animals	1390:1404	Medicurtain effectively prevented postlaparotomy adhesion in ∼42% of experimental animals, whereas ST 2.25-10, ST 2.5-5, ST 2.5-10, FT 20, and TST 1.5 were effective in 100% of animals.
33797224	6	49	theme	animals	1303:1309	arg1	%					1285:1285	∼42%	1282:1285	∼42% of experimental animals	1282:1309	Medicurtain effectively prevented postlaparotomy adhesion in ∼42% of experimental animals, whereas ST 2.25-10, ST 2.5-5, ST 2.5-10, FT 20, and TST 1.5 were effective in 100% of animals.
33797224	6	49	theme	animals	1303:1309	arg1	animals					1303:1309	experimental animals	1290:1309	experimental animals	1290:1309	Medicurtain effectively prevented postlaparotomy adhesion in ∼42% of experimental animals, whereas ST 2.25-10, ST 2.5-5, ST 2.5-10, FT 20, and TST 1.5 were effective in 100% of animals.
33797224	4	50	contain	contain	838:844	arg1	agents					826:831	These antiadhesion agents	807:831	These antiadhesion agents that contain no drugs	807:853	These antiadhesion agents that contain no drugs exhibit excellent physical characteristics and superior stability over 30 days in the operative sites without any toxicity and side effects that make the compositions strong candidates as novel antiadhesion agents.
33797224	4	50	contain	contain	838:844	arg2	drugs					849:853	no drugs	846:853	no drugs	846:853	These antiadhesion agents that contain no drugs exhibit excellent physical characteristics and superior stability over 30 days in the operative sites without any toxicity and side effects that make the compositions strong candidates as novel antiadhesion agents.
33797224	3	51	theme	agents	606:611	arg1	development					546:556	the development	542:556	the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST)	542:804	This article represents the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST).
33797224	4	52	dep	toxicity	969:976	arg1	any					965:967	any	965:967	any	965:967	These antiadhesion agents that contain no drugs exhibit excellent physical characteristics and superior stability over 30 days in the operative sites without any toxicity and side effects that make the compositions strong candidates as novel antiadhesion agents.
33797224	3	53	theme	characteristics	711:725	arg1	types					693:697	three different types	677:697	three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST)	677:804	This article represents the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST).
33797224	4	54	theme	superior	902:909	arg1	stability					911:919	superior stability	902:919	superior stability	902:919	These antiadhesion agents that contain no drugs exhibit excellent physical characteristics and superior stability over 30 days in the operative sites without any toxicity and side effects that make the compositions strong candidates as novel antiadhesion agents.
33797224	6	55	from	%	1393:1393	arg1	effective					1377:1385	effective	1377:1385	effective	1377:1385	Medicurtain effectively prevented postlaparotomy adhesion in ∼42% of experimental animals, whereas ST 2.25-10, ST 2.5-5, ST 2.5-10, FT 20, and TST 1.5 were effective in 100% of animals.
33797224	1	56	used	used	222:225	arg2	approaches					201:210	Pharmacological-based treatment approaches	169:210	Pharmacological-based treatment approaches	169:210	Pharmacological-based treatment approaches have been used over time to prevent postlaparotomy adhesion.
33797224	2	57	theme	side	360:363	arg1	effects					365:371	their unwanted side effects	345:371	their unwanted side effects	345:371	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	2	58	from	peritoneum	329:338	arg1	elimination					292:302	the rapid elimination	282:302	the rapid elimination of therapeutics from the peritoneum	282:338	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	2	58	from	peritoneum	329:338	arg1	effects					365:371	their unwanted side effects	345:371	their unwanted side effects	345:371	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	2	58	from	peritoneum	329:338	arg1	flow					379:382	easy flow	374:382	easy flow from the wound site by gravity	374:413	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	2	58	from	peritoneum	329:338	arg1	efficacy					436:443	low therapeutic efficacy	420:443	low therapeutic efficacy	420:443	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	5	59	from	antiadhesion	1113:1124	arg1	rats					1184:1187	Sprague-Dawley (SD) rats	1164:1187	Sprague-Dawley (SD) rats after surgery over Medicurtain	1164:1218	Also, the proposed samples reveal superior antiadhesion and tissue regeneration properties in Sprague-Dawley (SD) rats after surgery over Medicurtain.
33797224	2	60	theme	rapid	286:290	arg1	elimination					292:302	the rapid elimination	282:302	the rapid elimination of therapeutics from the peritoneum	282:338	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	2	61	theme	wound	393:397	arg1	site					399:402	the wound site	389:402	the wound site	389:402	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	3	62	theme	thermosensitive	779:793	arg1	TST					801:803	TST	801:803	TST	801:803	This article represents the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST).
33797224	3	62	theme	thermosensitive	779:793	arg1	type					795:798	thermosensitive type	779:798	thermosensitive type (TST)	779:804	This article represents the development of biocompatible and biodegradable antiadhesion agents that consist of carboxymethyl cellulose (CMC) and pullulan with three different types of physical characteristics such as the solution type (ST), film type (FT), and thermosensitive type (TST).
33797224	2	63	theme	agents	510:515	arg1	generation					483:492	the next generation	474:492	the next generation of antiadhesion agents	474:515	However, the rapid elimination of therapeutics from the peritoneum, and their unwanted side effects, easy flow from the wound site by gravity, and low therapeutic efficacy increase the urgent need for the next generation of antiadhesion agents.
33797224	4	64	theme	side	982:985	arg1	effects					987:993	side effects	982:993	side effects	982:993	These antiadhesion agents that contain no drugs exhibit excellent physical characteristics and superior stability over 30 days in the operative sites without any toxicity and side effects that make the compositions strong candidates as novel antiadhesion agents.
33797224	7	65	theme	further	1576:1582	arg1	study					1584:1588	further study	1576:1588	further study	1576:1588	Thus, we believe these antiadhesion agents could be promising to reduce adhesion-related complications during and post-surgical operations and deserve consideration for further study for clinical purposes.
33797224	1	66	theme	Pharmacological-based	169:189	arg1	approaches					201:210	Pharmacological-based treatment approaches	169:210	Pharmacological-based treatment approaches	169:210	Pharmacological-based treatment approaches have been used over time to prevent postlaparotomy adhesion.
33797224	4	67	theme	antiadhesion	1049:1060	arg1	agents					1062:1067	novel antiadhesion agents	1043:1067	novel antiadhesion agents	1043:1067	These antiadhesion agents that contain no drugs exhibit excellent physical characteristics and superior stability over 30 days in the operative sites without any toxicity and side effects that make the compositions strong candidates as novel antiadhesion agents.
33797224	5	68	theme	Sprague-Dawley	1164:1177	arg1	rats					1184:1187	Sprague-Dawley (SD) rats	1164:1187	Sprague-Dawley (SD) rats after surgery over Medicurtain	1164:1218	Also, the proposed samples reveal superior antiadhesion and tissue regeneration properties in Sprague-Dawley (SD) rats after surgery over Medicurtain.
33183613	1	0	theme	infections	175:184	arg1	difficulty					151:160	treatment difficulty	141:160	treatment difficulty of S. aureus infections	141:184	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	0	1	theme	enrofloxacin	98:109	arg1	release					111:117	dual responsive enrofloxacin release	82:117	dual responsive enrofloxacin release	82:117	Composite inclusion complexes containing hyaluronic acid/chitosan nanosystems for dual responsive enrofloxacin release.
33183613	3	2	theme	responsive	696:705	arg1	release					707:713	responsive release	696:713	responsive release	696:713	The optimal nanosystems of 118.8 ± 30.7 nm showed excellent stability and responsive release in the acid medium, hyaluronidase containing medium, and LB broth medium where S. aureus present.
33183613	5	3	theme	single	1034:1039	arg1	nanogels					1047:1054	the single HA/CS nanogels	1030:1054	the single HA/CS nanogels	1030:1054	It had stronger sustained release than the polymeric nanoparticles formulated by entrapping of IC into F68 and the single HA/CS nanogels.
33183613	6	4	theme	aureus	1164:1169	arg1	challenge					1148:1156	the treatment challenge	1134:1156	the treatment challenge of S. aureus and other bacterial infections	1134:1200	This study provides a promising multi-functionalized nanosystems to overcome the treatment challenge of S. aureus and other bacterial infections.
33183613	6	5	theme	treatment	1138:1146	arg1	challenge					1148:1156	the treatment challenge	1134:1156	the treatment challenge of S. aureus and other bacterial infections	1134:1200	This study provides a promising multi-functionalized nanosystems to overcome the treatment challenge of S. aureus and other bacterial infections.
33183613	3	6	theme	hyaluronidase	735:747	arg1	medium					760:765	hyaluronidase containing medium	735:765	hyaluronidase containing medium	735:765	The optimal nanosystems of 118.8 ± 30.7 nm showed excellent stability and responsive release in the acid medium, hyaluronidase containing medium, and LB broth medium where S. aureus present.
33183613	6	7	theme	multi-functionalized	1089:1108	arg1	nanosystems					1110:1120	a promising multi-functionalized nanosystems	1077:1120	a promising multi-functionalized nanosystems to overcome the treatment challenge of S. aureus and other bacterial infections	1077:1200	This study provides a promising multi-functionalized nanosystems to overcome the treatment challenge of S. aureus and other bacterial infections.
33183613	3	8	theme	acid	722:725	arg1	medium					727:732	the acid medium	718:732	the acid medium	718:732	The optimal nanosystems of 118.8 ± 30.7 nm showed excellent stability and responsive release in the acid medium, hyaluronidase containing medium, and LB broth medium where S. aureus present.
33183613	4	9	theme	surface	846:852	arg1	adsorption					854:863	strong surface adsorption	839:863	strong surface adsorption	839:863	The nanosystems displayed strong surface adsorption on S. aureus and enhanced activity against S. aureus.
33183613	6	10	theme	infections	1191:1200	arg1	challenge					1148:1156	the treatment challenge	1134:1156	the treatment challenge of S. aureus and other bacterial infections	1134:1200	This study provides a promising multi-functionalized nanosystems to overcome the treatment challenge of S. aureus and other bacterial infections.
33183613	1	11	theme	pH/hyaluronidase	189:204	arg1	β-CD					249:252	β-CD	249:252	β-CD	249:252	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	1	11	theme	pH/hyaluronidase	189:204	arg1	enrofloxacin-cyclodextrin					222:246	a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin	187:246	a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68)	187:389	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	0	12	theme	inclusion	10:18	arg1	complexes					20:28	Composite inclusion complexes	0:28	Composite inclusion complexes	0:28	Composite inclusion complexes containing hyaluronic acid/chitosan nanosystems for dual responsive enrofloxacin release.
33183613	6	13	theme	bacterial	1181:1189	arg1	infections					1191:1200	other bacterial infections	1175:1200	other bacterial infections	1175:1200	This study provides a promising multi-functionalized nanosystems to overcome the treatment challenge of S. aureus and other bacterial infections.
33183613	1	14	theme	dual	206:209	arg1	β-CD					249:252	β-CD	249:252	β-CD	249:252	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	1	14	theme	dual	206:209	arg1	enrofloxacin-cyclodextrin					222:246	a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin	187:246	a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68)	187:389	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	0	15	theme	Composite	0:8	arg1	complexes					20:28	Composite inclusion complexes	0:28	Composite inclusion complexes	0:28	Composite inclusion complexes containing hyaluronic acid/chitosan nanosystems for dual responsive enrofloxacin release.
33183613	6	16	theme	other	1175:1179	arg1	infections					1191:1200	other bacterial infections	1175:1200	other bacterial infections	1175:1200	This study provides a promising multi-functionalized nanosystems to overcome the treatment challenge of S. aureus and other bacterial infections.
33183613	1	17	theme	responsive	211:220	arg1	β-CD					249:252	β-CD	249:252	β-CD	249:252	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	1	17	theme	responsive	211:220	arg1	enrofloxacin-cyclodextrin					222:246	a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin	187:246	a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68)	187:389	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	3	18	theme	nm	662:663	arg1	nanosystems					634:644	The optimal nanosystems	622:644	The optimal nanosystems of 118.8 ± 30.7 nm	622:663	The optimal nanosystems of 118.8 ± 30.7 nm showed excellent stability and responsive release in the acid medium, hyaluronidase containing medium, and LB broth medium where S. aureus present.
33183613	1	19	theme	enrofloxacin-cyclodextrin	222:246	arg1	IC					276:277	IC	276:277	IC	276:277	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	1	19	theme	enrofloxacin-cyclodextrin	222:246	arg1	complexes					265:273	a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes	187:273	a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68)	187:389	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	3	20	theme	containing	749:758	arg1	medium					760:765	hyaluronidase containing medium	735:765	hyaluronidase containing medium	735:765	The optimal nanosystems of 118.8 ± 30.7 nm showed excellent stability and responsive release in the acid medium, hyaluronidase containing medium, and LB broth medium where S. aureus present.
33183613	5	21	theme	polymeric	962:970	arg1	nanoparticles					972:984	the polymeric nanoparticles	958:984	the polymeric nanoparticles formulated by entrapping of IC into F68 and the single HA/CS nanogels	958:1054	It had stronger sustained release than the polymeric nanoparticles formulated by entrapping of IC into F68 and the single HA/CS nanogels.
33183613	0	22	theme	acid/chitosan	52:64	arg1	nanosystems					66:76	hyaluronic acid/chitosan nanosystems	41:76	hyaluronic acid/chitosan nanosystems	41:76	Composite inclusion complexes containing hyaluronic acid/chitosan nanosystems for dual responsive enrofloxacin release.
33183613	1	23	theme	self-assemble	324:336	arg1	nanosystems					348:358	hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems	291:358	hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68)	291:389	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	2	24	theme	coating	546:552	arg1	nanogels					554:561	F68 coating nanogels	542:561	F68 coating nanogels formulated by electrostatic interaction between CS and HA	542:619	The FTIR, DSC and PXRD showed that enrofloxacin was embedded into IC and then distributed into F68 coating nanogels formulated by electrostatic interaction between CS and HA.
33183613	1	25	theme	targeted	416:423	arg1	delivery					437:444	targeted "on-demand" delivery	416:444	targeted "on-demand" delivery	416:444	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	0	26	theme	hyaluronic	41:50	arg1	nanosystems					66:76	hyaluronic acid/chitosan nanosystems	41:76	hyaluronic acid/chitosan nanosystems	41:76	Composite inclusion complexes containing hyaluronic acid/chitosan nanosystems for dual responsive enrofloxacin release.
33183613	1	27	theme	composite	338:346	arg1	nanosystems					348:358	hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems	291:358	hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68)	291:389	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	2	28	theme	F68	542:544	arg1	nanogels					554:561	F68 coating nanogels	542:561	F68 coating nanogels formulated by electrostatic interaction between CS and HA	542:619	The FTIR, DSC and PXRD showed that enrofloxacin was embedded into IC and then distributed into F68 coating nanogels formulated by electrostatic interaction between CS and HA.
33183613	1	29	theme	on-demand	426:434	arg1	delivery					437:444	targeted "on-demand" delivery	416:444	targeted "on-demand" delivery	416:444	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	4	30	theme	strong	839:844	arg1	adsorption					854:863	strong surface adsorption	839:863	strong surface adsorption	839:863	The nanosystems displayed strong surface adsorption on S. aureus and enhanced activity against S. aureus.
33183613	3	31	theme	LB	772:773	arg1	medium					781:786	LB broth medium	772:786	LB broth medium	772:786	The optimal nanosystems of 118.8 ± 30.7 nm showed excellent stability and responsive release in the acid medium, hyaluronidase containing medium, and LB broth medium where S. aureus present.
33183613	1	32	theme	"	435:435	arg1	delivery					437:444	targeted "on-demand" delivery	416:444	targeted "on-demand" delivery	416:444	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	5	33	theme	HA/CS	1041:1045	arg1	nanogels					1047:1054	the single HA/CS nanogels	1030:1054	the single HA/CS nanogels	1030:1054	It had stronger sustained release than the polymeric nanoparticles formulated by entrapping of IC into F68 and the single HA/CS nanogels.
33183613	5	34	contain	had	922:924	arg2	release					945:951	stronger sustained release	926:951	stronger sustained release	926:951	It had stronger sustained release than the polymeric nanoparticles formulated by entrapping of IC into F68 and the single HA/CS nanogels.
33183613	5	34	contain	had	922:924	arg1	It					919:920	It	919:920	It	919:920	It had stronger sustained release than the polymeric nanoparticles formulated by entrapping of IC into F68 and the single HA/CS nanogels.
33183613	1	35	theme	treatment	141:149	arg1	difficulty					151:160	treatment difficulty	141:160	treatment difficulty of S. aureus infections	141:184	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	3	36	theme	broth	775:779	arg1	medium					781:786	LB broth medium	772:786	LB broth medium	772:786	The optimal nanosystems of 118.8 ± 30.7 nm showed excellent stability and responsive release in the acid medium, hyaluronidase containing medium, and LB broth medium where S. aureus present.
33183613	2	37	theme	electrostatic	577:589	arg1	interaction					591:601	electrostatic interaction	577:601	electrostatic interaction between CS and HA	577:619	The FTIR, DSC and PXRD showed that enrofloxacin was embedded into IC and then distributed into F68 coating nanogels formulated by electrostatic interaction between CS and HA.
33183613	5	38	theme	stronger	926:933	arg1	release					945:951	stronger sustained release	926:951	stronger sustained release	926:951	It had stronger sustained release than the polymeric nanoparticles formulated by entrapping of IC into F68 and the single HA/CS nanogels.
33183613	1	39	theme	inclusion	255:263	arg1	IC					276:277	IC	276:277	IC	276:277	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	1	39	theme	inclusion	255:263	arg1	complexes					265:273	a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes	187:273	a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68)	187:389	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	5	40	theme	sustained	935:943	arg1	release					945:951	stronger sustained release	926:951	stronger sustained release	926:951	It had stronger sustained release than the polymeric nanoparticles formulated by entrapping of IC into F68 and the single HA/CS nanogels.
33183613	0	41	theme	responsive	87:96	arg1	release					111:117	dual responsive enrofloxacin release	82:117	dual responsive enrofloxacin release	82:117	Composite inclusion complexes containing hyaluronic acid/chitosan nanosystems for dual responsive enrofloxacin release.
33183613	3	42	theme	excellent	672:680	arg1	stability					682:690	excellent stability	672:690	excellent stability	672:690	The optimal nanosystems of 118.8 ± 30.7 nm showed excellent stability and responsive release in the acid medium, hyaluronidase containing medium, and LB broth medium where S. aureus present.
33183613	1	43	contain	containing	280:289	arg2	nanosystems					348:358	hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems	291:358	hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68)	291:389	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	1	43	contain	containing	280:289	arg1	IC					276:277	IC	276:277	IC	276:277	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	1	43	contain	containing	280:289	arg1	complexes					265:273	a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes	187:273	a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68)	187:389	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	1	44	theme	S.	165:166	arg1	infections					175:184	S. aureus infections	165:184	S. aureus infections	165:184	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	6	45	theme	promising	1079:1087	arg1	nanosystems					1110:1120	a promising multi-functionalized nanosystems	1077:1120	a promising multi-functionalized nanosystems to overcome the treatment challenge of S. aureus and other bacterial infections	1077:1200	This study provides a promising multi-functionalized nanosystems to overcome the treatment challenge of S. aureus and other bacterial infections.
33183613	0	46	theme	dual	82:85	arg1	release					111:117	dual responsive enrofloxacin release	82:117	dual responsive enrofloxacin release	82:117	Composite inclusion complexes containing hyaluronic acid/chitosan nanosystems for dual responsive enrofloxacin release.
33183613	1	47	theme	poloxamer	371:379	arg1	F68					386:388	F68	386:388	F68	386:388	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	1	47	theme	poloxamer	371:379	arg1	188					381:383	poloxamer 188	371:383	poloxamer 188 (F68)	371:389	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
33183613	3	48	theme	optimal	626:632	arg1	nanosystems					634:644	The optimal nanosystems	622:644	The optimal nanosystems of 118.8 ± 30.7 nm	622:663	The optimal nanosystems of 118.8 ± 30.7 nm showed excellent stability and responsive release in the acid medium, hyaluronidase containing medium, and LB broth medium where S. aureus present.
33183613	1	49	theme	aureus	168:173	arg1	infections					175:184	S. aureus infections	165:184	S. aureus infections	165:184	In order to overcome treatment difficulty of S. aureus infections, a pH/hyaluronidase dual responsive enrofloxacin-cyclodextrin (β-CD) inclusion complexes (IC) containing hyaluronic acid/chitosan (HA/CS) self-assemble composite nanosystems covered by poloxamer 188 (F68) was firstly explored for targeted "on-demand" delivery.
34865793	3	0	theme	MPCs	633:636	arg1	addition					599:606	the addition	595:606	the addition of untreated and treated MPCs	595:636	The results showed that the addition of untreated and treated MPCs reduced the hardness, the stiffness and the solid-like behavior of the alginate gels.
34865793	2	1	theme	surface	531:537	arg1	morphology					539:548	surface morphology	531:548	surface morphology	531:548	The gels were characterized for the degree of syneresis, swelling behavior, hardness, stiffness, viscoelastic behavior, and surface morphology of freeze-dried gel.
34865793	9	2	theme	composite	1386:1394	arg1	gels					1396:1399	only cold-renneted MPC composite gels	1363:1399	only cold-renneted MPC composite gels	1363:1399	Moreover, only cold-renneted MPC composite gels showed lower shrinkage and wrinkles on the surface of the beads during lyophilization.
34865793	7	3	theme	simulated	1089:1097	arg1	SIF					1117:1119	SIF	1117:1119	SIF	1117:1119	After incubation in simulated intestinal fluid (SIF), the gel containing alginate only swelled while gels containing untreated MPC and pre-heated MPC experienced degradation and a severe mass loss.
34865793	7	3	theme	simulated	1089:1097	arg1	fluid					1110:1114	simulated intestinal fluid	1089:1114	simulated intestinal fluid (SIF)	1089:1120	After incubation in simulated intestinal fluid (SIF), the gel containing alginate only swelled while gels containing untreated MPC and pre-heated MPC experienced degradation and a severe mass loss.
34865793	3	4	theme	untreated	611:619	arg1	MPCs					633:636	untreated and treated MPCs	611:636	untreated and treated MPCs	611:636	The results showed that the addition of untreated and treated MPCs reduced the hardness, the stiffness and the solid-like behavior of the alginate gels.
34865793	1	5	dep	untreated	201:209	arg1	30 min					250:255	30 min	250:255	30 min	250:255	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	1	5	dep	untreated	201:209	arg1	80 °C					243:247	80 °C	243:247	80 °C	243:247	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	1	6	theme	w/w	329:331	arg1	gels					344:347	sodium alginate (2% w/w) composite gels	309:347	sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms	309:404	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	1	6	theme	w/w	329:331	arg1	%					327:327	2% w/w	326:331	2% w/w	326:331	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	1	7	theme	sodium	309:314	arg1	gels					344:347	sodium alginate (2% w/w) composite gels	309:347	sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms	309:404	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	1	7	theme	sodium	309:314	arg1	%					327:327	2% w/w	326:331	2% w/w	326:331	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	4	8	theme	gels	799:802	arg1	syneresis					777:785	syneresis	777:785	syneresis of alginate gels	777:802	Untreated MPC and pre-heated MPC caused no effect on syneresis of alginate gels.
34865793	5	9	theme	composite	874:882	arg1	gels					884:887	composite gels	874:887	composite gels	874:887	The addition of cold-renneted MPC reduced the degree of syneresis in composite gels by 13.7%.
34865793	1	10	from	gels	344:347	arg1	forms					400:404	millimeter-size (bead) and centrimeter-size gel forms	352:404	millimeter-size (bead) and centrimeter-size gel forms	352:404	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	1	11	theme	alginate	316:323	arg1	gels					344:347	sodium alginate (2% w/w) composite gels	309:347	sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms	309:404	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	1	11	theme	alginate	316:323	arg1	%					327:327	2% w/w	326:331	2% w/w	326:331	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	4	12	theme	alginate	790:797	arg1	gels					799:802	alginate gels	790:802	alginate gels	790:802	Untreated MPC and pre-heated MPC caused no effect on syneresis of alginate gels.
34865793	1	13	theme	centrimeter-size	379:394	arg1	forms					400:404	millimeter-size (bead) and centrimeter-size gel forms	352:404	millimeter-size (bead) and centrimeter-size gel forms	352:404	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	9	14	theme	lower	1408:1412	arg1	shrinkage					1414:1422	lower shrinkage	1408:1422	lower shrinkage	1408:1422	Moreover, only cold-renneted MPC composite gels showed lower shrinkage and wrinkles on the surface of the beads during lyophilization.
34865793	10	15	theme	gelation	1564:1571	arg1	method					1573:1578	cold-rennet induced gelation method	1544:1578	cold-rennet induced gelation method	1544:1578	Therefore, based on these results, it is indicated that cold-rennet induced gelation method could increase the effectiveness of MPC as a composite material for alginate gels.
34865793	1	16	dep	millimeter-size	352:366	arg1	bead					369:372	bead	369:372	bead	369:372	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	4	17	from	effect	767:772	arg1	syneresis					777:785	syneresis	777:785	syneresis of alginate gels	777:802	Untreated MPC and pre-heated MPC caused no effect on syneresis of alginate gels.
34865793	2	18	theme	gel	566:568	arg1	stiffness					493:501	stiffness	493:501	stiffness	493:501	The gels were characterized for the degree of syneresis, swelling behavior, hardness, stiffness, viscoelastic behavior, and surface morphology of freeze-dried gel.
34865793	2	18	theme	gel	566:568	arg1	behavior					517:524	viscoelastic behavior	504:524	viscoelastic behavior	504:524	The gels were characterized for the degree of syneresis, swelling behavior, hardness, stiffness, viscoelastic behavior, and surface morphology of freeze-dried gel.
34865793	2	18	theme	gel	566:568	arg1	hardness					483:490	hardness	483:490	hardness	483:490	The gels were characterized for the degree of syneresis, swelling behavior, hardness, stiffness, viscoelastic behavior, and surface morphology of freeze-dried gel.
34865793	2	18	theme	gel	566:568	arg1	behavior					473:480	behavior	473:480	behavior	473:480	The gels were characterized for the degree of syneresis, swelling behavior, hardness, stiffness, viscoelastic behavior, and surface morphology of freeze-dried gel.
34865793	2	18	theme	gel	566:568	arg1	morphology					539:548	surface morphology	531:548	surface morphology	531:548	The gels were characterized for the degree of syneresis, swelling behavior, hardness, stiffness, viscoelastic behavior, and surface morphology of freeze-dried gel.
34865793	1	19	theme	gel	396:398	arg1	forms					400:404	millimeter-size (bead) and centrimeter-size gel forms	352:404	millimeter-size (bead) and centrimeter-size gel forms	352:404	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	7	20	theme	pre-heated	1204:1213	arg1	MPC					1215:1217	pre-heated MPC	1204:1217	pre-heated MPC	1204:1217	After incubation in simulated intestinal fluid (SIF), the gel containing alginate only swelled while gels containing untreated MPC and pre-heated MPC experienced degradation and a severe mass loss.
34865793	1	21	from	properties	295:304	arg1	forms					400:404	millimeter-size (bead) and centrimeter-size gel forms	352:404	millimeter-size (bead) and centrimeter-size gel forms	352:404	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	10	22	theme	cold-rennet	1544:1554	arg1	method					1573:1578	cold-rennet induced gelation method	1544:1578	cold-rennet induced gelation method	1544:1578	Therefore, based on these results, it is indicated that cold-rennet induced gelation method could increase the effectiveness of MPC as a composite material for alginate gels.
34865793	2	23	theme	freeze-dried	553:564	arg1	gel					566:568	freeze-dried gel	553:568	freeze-dried gel	553:568	The gels were characterized for the degree of syneresis, swelling behavior, hardness, stiffness, viscoelastic behavior, and surface morphology of freeze-dried gel.
34865793	6	24	theme	gastric	1048:1054	arg1	fluid					1056:1060	simulated gastric fluid	1038:1060	simulated gastric fluid (SGF)	1038:1066	Similarly, the addition of cold-renneted MPC reduced (by 16.4%) the degree of syneresis of the alginate composite gels after incubation in simulated gastric fluid (SGF).
34865793	6	24	theme	gastric	1048:1054	arg1	SGF					1063:1065	SGF	1063:1065	SGF	1063:1065	Similarly, the addition of cold-renneted MPC reduced (by 16.4%) the degree of syneresis of the alginate composite gels after incubation in simulated gastric fluid (SGF).
34865793	8	25	dep	swelled	1316:1322	arg1	eroded					1345:1350	eroded	1345:1350	eroded	1345:1350	Meanwhile, the gels containing cold-renneted MPC swelled and at the same time eroded.
34865793	8	26	contain	containing	1287:1296	arg1	Meanwhile					1267:1275	Meanwhile	1267:1275	Meanwhile	1267:1275	Meanwhile, the gels containing cold-renneted MPC swelled and at the same time eroded.
34865793	8	26	contain	containing	1287:1296	arg1	gels					1282:1285	the gels	1278:1285	the gels containing cold-renneted MPC	1278:1314	Meanwhile, the gels containing cold-renneted MPC swelled and at the same time eroded.
34865793	8	26	contain	containing	1287:1296	arg2	MPC					1312:1314	cold-renneted MPC	1298:1314	cold-renneted MPC	1298:1314	Meanwhile, the gels containing cold-renneted MPC swelled and at the same time eroded.
34865793	6	27	theme	simulated	1038:1046	arg1	fluid					1056:1060	simulated gastric fluid	1038:1060	simulated gastric fluid (SGF)	1038:1066	Similarly, the addition of cold-renneted MPC reduced (by 16.4%) the degree of syneresis of the alginate composite gels after incubation in simulated gastric fluid (SGF).
34865793	6	27	theme	simulated	1038:1046	arg1	SGF					1063:1065	SGF	1063:1065	SGF	1063:1065	Similarly, the addition of cold-renneted MPC reduced (by 16.4%) the degree of syneresis of the alginate composite gels after incubation in simulated gastric fluid (SGF).
34865793	1	28	theme	milk	167:170	arg1	MPC85					193:197	MPC85	193:197	MPC85	193:197	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	1	28	theme	milk	167:170	arg1	concentrate					180:190	milk protein concentrate	167:190	milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition	167:266	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	6	29	theme	gels	1013:1016	arg1	syneresis					977:985	syneresis	977:985	syneresis of the alginate composite gels	977:1016	Similarly, the addition of cold-renneted MPC reduced (by 16.4%) the degree of syneresis of the alginate composite gels after incubation in simulated gastric fluid (SGF).
34865793	8	30	theme	same	1335:1338	arg1	time					1340:1343	the same time	1331:1343	the same time	1331:1343	Meanwhile, the gels containing cold-renneted MPC swelled and at the same time eroded.
34865793	6	31	from	incubation	1024:1033	arg1	fluid					1056:1060	simulated gastric fluid	1038:1060	simulated gastric fluid (SGF)	1038:1066	Similarly, the addition of cold-renneted MPC reduced (by 16.4%) the degree of syneresis of the alginate composite gels after incubation in simulated gastric fluid (SGF).
34865793	6	31	from	incubation	1024:1033	arg1	SGF					1063:1065	SGF	1063:1065	SGF	1063:1065	Similarly, the addition of cold-renneted MPC reduced (by 16.4%) the degree of syneresis of the alginate composite gels after incubation in simulated gastric fluid (SGF).
34865793	1	32	theme	protein	172:178	arg1	MPC85					193:197	MPC85	193:197	MPC85	193:197	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	1	32	theme	protein	172:178	arg1	concentrate					180:190	milk protein concentrate	167:190	milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition	167:266	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	4	33	theme	Untreated	724:732	arg1	MPC					734:736	Untreated MPC	724:736	Untreated MPC	724:736	Untreated MPC and pre-heated MPC caused no effect on syneresis of alginate gels.
34865793	0	34	theme	composite	112:120	arg1	gels					122:125	alginate composite gels	103:125	alginate composite gels	103:125	Effects of cold-renneted and pre-heated milk protein concentrates (MPCs) addition on the properties of alginate composite gels.
34865793	10	35	theme	alginate	1648:1655	arg1	gels					1657:1660	alginate gels	1648:1660	alginate gels	1648:1660	Therefore, based on these results, it is indicated that cold-rennet induced gelation method could increase the effectiveness of MPC as a composite material for alginate gels.
34865793	5	36	theme	syneresis	861:869	arg1	degree					851:856	the degree	847:856	the degree of syneresis in composite gels	847:887	The addition of cold-renneted MPC reduced the degree of syneresis in composite gels by 13.7%.
34865793	1	37	theme	concentrate	180:190	arg1	addition					259:266	milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition	167:266	milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition	167:266	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	0	38	theme	cold-renneted	11:23	arg1	MPCs					67:70	MPCs	67:70	MPCs	67:70	Effects of cold-renneted and pre-heated milk protein concentrates (MPCs) addition on the properties of alginate composite gels.
34865793	0	38	theme	cold-renneted	11:23	arg1	concentrates					53:64	cold-renneted and pre-heated milk protein concentrates	11:64	cold-renneted and pre-heated milk protein concentrates (MPCs)	11:71	Effects of cold-renneted and pre-heated milk protein concentrates (MPCs) addition on the properties of alginate composite gels.
34865793	0	39	theme	alginate	103:110	arg1	gels					122:125	alginate composite gels	103:125	alginate composite gels	103:125	Effects of cold-renneted and pre-heated milk protein concentrates (MPCs) addition on the properties of alginate composite gels.
34865793	0	40	from	addition	73:80	arg1	properties					89:98	the properties	85:98	the properties of alginate composite gels	85:125	Effects of cold-renneted and pre-heated milk protein concentrates (MPCs) addition on the properties of alginate composite gels.
34865793	10	41	theme	MPC	1616:1618	arg1	effectiveness					1599:1611	the effectiveness	1595:1611	the effectiveness of MPC	1595:1618	Therefore, based on these results, it is indicated that cold-rennet induced gelation method could increase the effectiveness of MPC as a composite material for alginate gels.
34865793	10	41	theme	MPC	1616:1618	arg1	material					1635:1642	a composite material	1623:1642	a composite material for alginate gels	1623:1660	Therefore, based on these results, it is indicated that cold-rennet induced gelation method could increase the effectiveness of MPC as a composite material for alginate gels.
34865793	8	42	theme	cold-renneted	1298:1310	arg1	MPC					1312:1314	cold-renneted MPC	1298:1314	cold-renneted MPC	1298:1314	Meanwhile, the gels containing cold-renneted MPC swelled and at the same time eroded.
34865793	0	43	theme	milk	40:43	arg1	MPCs					67:70	MPCs	67:70	MPCs	67:70	Effects of cold-renneted and pre-heated milk protein concentrates (MPCs) addition on the properties of alginate composite gels.
34865793	0	43	theme	milk	40:43	arg1	concentrates					53:64	cold-renneted and pre-heated milk protein concentrates	11:64	cold-renneted and pre-heated milk protein concentrates (MPCs)	11:71	Effects of cold-renneted and pre-heated milk protein concentrates (MPCs) addition on the properties of alginate composite gels.
34865793	6	44	theme	composite	1003:1011	arg1	gels					1013:1016	the alginate composite gels	990:1016	the alginate composite gels	990:1016	Similarly, the addition of cold-renneted MPC reduced (by 16.4%) the degree of syneresis of the alginate composite gels after incubation in simulated gastric fluid (SGF).
34865793	7	45	theme	severe	1249:1254	arg1	loss					1261:1264	a severe mass loss	1247:1264	a severe mass loss	1247:1264	After incubation in simulated intestinal fluid (SIF), the gel containing alginate only swelled while gels containing untreated MPC and pre-heated MPC experienced degradation and a severe mass loss.
34865793	7	46	theme	untreated	1186:1194	arg1	MPC					1196:1198	untreated MPC	1186:1198	untreated MPC	1186:1198	After incubation in simulated intestinal fluid (SIF), the gel containing alginate only swelled while gels containing untreated MPC and pre-heated MPC experienced degradation and a severe mass loss.
34865793	0	47	theme	pre-heated	29:38	arg1	MPCs					67:70	MPCs	67:70	MPCs	67:70	Effects of cold-renneted and pre-heated milk protein concentrates (MPCs) addition on the properties of alginate composite gels.
34865793	0	47	theme	pre-heated	29:38	arg1	concentrates					53:64	cold-renneted and pre-heated milk protein concentrates	11:64	cold-renneted and pre-heated milk protein concentrates (MPCs)	11:71	Effects of cold-renneted and pre-heated milk protein concentrates (MPCs) addition on the properties of alginate composite gels.
34865793	0	48	theme	gels	122:125	arg1	properties					89:98	the properties	85:98	the properties of alginate composite gels	85:125	Effects of cold-renneted and pre-heated milk protein concentrates (MPCs) addition on the properties of alginate composite gels.
34865793	1	49	theme	composite	334:342	arg1	gels					344:347	sodium alginate (2% w/w) composite gels	309:347	sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms	309:404	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	1	49	theme	composite	334:342	arg1	%					327:327	2% w/w	326:331	2% w/w	326:331	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	7	50	theme	mass	1256:1259	arg1	loss					1261:1264	a severe mass loss	1247:1264	a severe mass loss	1247:1264	After incubation in simulated intestinal fluid (SIF), the gel containing alginate only swelled while gels containing untreated MPC and pre-heated MPC experienced degradation and a severe mass loss.
34865793	6	51	theme	MPC	940:942	arg1	addition					914:921	the addition	910:921	the addition of cold-renneted MPC	910:942	Similarly, the addition of cold-renneted MPC reduced (by 16.4%) the degree of syneresis of the alginate composite gels after incubation in simulated gastric fluid (SGF).
34865793	0	52	theme	concentrates	53:64	arg1	Effects					0:6	Effects	0:6	Effects of cold-renneted and pre-heated milk protein concentrates (MPCs)	0:71	Effects of cold-renneted and pre-heated milk protein concentrates (MPCs) addition on the properties of alginate composite gels.
34865793	1	53	theme	addition	259:266	arg1	effects					156:162	the effects	152:162	the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms	152:404	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	2	54	theme	viscoelastic	504:515	arg1	behavior					517:524	viscoelastic behavior	504:524	viscoelastic behavior	504:524	The gels were characterized for the degree of syneresis, swelling behavior, hardness, stiffness, viscoelastic behavior, and surface morphology of freeze-dried gel.
34865793	1	55	theme	gels	344:347	arg1	properties					295:304	the physical/mechanical properties	271:304	the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms	271:404	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	9	56	theme	beads	1459:1463	arg1	surface					1444:1450	the surface	1440:1450	the surface of the beads during lyophilization	1440:1485	Moreover, only cold-renneted MPC composite gels showed lower shrinkage and wrinkles on the surface of the beads during lyophilization.
34865793	5	57	theme	cold-renneted	821:833	arg1	MPC					835:837	cold-renneted MPC	821:837	cold-renneted MPC	821:837	The addition of cold-renneted MPC reduced the degree of syneresis in composite gels by 13.7%.
34865793	0	58	theme	protein	45:51	arg1	MPCs					67:70	MPCs	67:70	MPCs	67:70	Effects of cold-renneted and pre-heated milk protein concentrates (MPCs) addition on the properties of alginate composite gels.
34865793	0	58	theme	protein	45:51	arg1	concentrates					53:64	cold-renneted and pre-heated milk protein concentrates	11:64	cold-renneted and pre-heated milk protein concentrates (MPCs)	11:71	Effects of cold-renneted and pre-heated milk protein concentrates (MPCs) addition on the properties of alginate composite gels.
34865793	3	59	theme	solid-like	682:691	arg1	behavior					693:700	the solid-like behavior	678:700	the solid-like behavior of the alginate gels	678:721	The results showed that the addition of untreated and treated MPCs reduced the hardness, the stiffness and the solid-like behavior of the alginate gels.
34865793	3	60	theme	treated	625:631	arg1	MPCs					633:636	untreated and treated MPCs	611:636	untreated and treated MPCs	611:636	The results showed that the addition of untreated and treated MPCs reduced the hardness, the stiffness and the solid-like behavior of the alginate gels.
34865793	7	61	contain	containing	1175:1184	arg2	MPC					1196:1198	untreated MPC	1186:1198	untreated MPC	1186:1198	After incubation in simulated intestinal fluid (SIF), the gel containing alginate only swelled while gels containing untreated MPC and pre-heated MPC experienced degradation and a severe mass loss.
34865793	7	61	contain	containing	1175:1184	arg1	gels					1170:1173	gels	1170:1173	gels containing untreated MPC and pre-heated MPC	1170:1217	After incubation in simulated intestinal fluid (SIF), the gel containing alginate only swelled while gels containing untreated MPC and pre-heated MPC experienced degradation and a severe mass loss.
34865793	7	61	contain	containing	1175:1184	arg2	MPC					1215:1217	pre-heated MPC	1204:1217	pre-heated MPC	1204:1217	After incubation in simulated intestinal fluid (SIF), the gel containing alginate only swelled while gels containing untreated MPC and pre-heated MPC experienced degradation and a severe mass loss.
34865793	4	62	theme	pre-heated	742:751	arg1	MPC					753:755	pre-heated MPC	742:755	pre-heated MPC	742:755	Untreated MPC and pre-heated MPC caused no effect on syneresis of alginate gels.
34865793	7	63	theme	containing	1131:1140	arg1	alginate					1142:1149	the gel containing alginate	1123:1149	the gel containing alginate	1123:1149	After incubation in simulated intestinal fluid (SIF), the gel containing alginate only swelled while gels containing untreated MPC and pre-heated MPC experienced degradation and a severe mass loss.
34865793	5	64	theme	MPC	835:837	arg1	addition					809:816	The addition	805:816	The addition of cold-renneted MPC	805:837	The addition of cold-renneted MPC reduced the degree of syneresis in composite gels by 13.7%.
34865793	1	65	theme	millimeter-size	352:366	arg1	forms					400:404	millimeter-size (bead) and centrimeter-size gel forms	352:404	millimeter-size (bead) and centrimeter-size gel forms	352:404	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	9	66	theme	MPC	1382:1384	arg1	gels					1396:1399	only cold-renneted MPC composite gels	1363:1399	only cold-renneted MPC composite gels	1363:1399	Moreover, only cold-renneted MPC composite gels showed lower shrinkage and wrinkles on the surface of the beads during lyophilization.
34865793	1	67	from	forms	400:404	arg1	properties					295:304	the physical/mechanical properties	271:304	the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms	271:404	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	10	68	theme	composite	1625:1633	arg1	effectiveness					1599:1611	the effectiveness	1595:1611	the effectiveness of MPC	1595:1618	Therefore, based on these results, it is indicated that cold-rennet induced gelation method could increase the effectiveness of MPC as a composite material for alginate gels.
34865793	10	68	theme	composite	1625:1633	arg1	material					1635:1642	a composite material	1623:1642	a composite material for alginate gels	1623:1660	Therefore, based on these results, it is indicated that cold-rennet induced gelation method could increase the effectiveness of MPC as a composite material for alginate gels.
34865793	6	69	theme	cold-renneted	926:938	arg1	MPC					940:942	cold-renneted MPC	926:942	cold-renneted MPC	926:942	Similarly, the addition of cold-renneted MPC reduced (by 16.4%) the degree of syneresis of the alginate composite gels after incubation in simulated gastric fluid (SGF).
34865793	7	70	from	incubation	1075:1084	arg1	SIF					1117:1119	SIF	1117:1119	SIF	1117:1119	After incubation in simulated intestinal fluid (SIF), the gel containing alginate only swelled while gels containing untreated MPC and pre-heated MPC experienced degradation and a severe mass loss.
34865793	7	70	from	incubation	1075:1084	arg1	fluid					1110:1114	simulated intestinal fluid	1089:1114	simulated intestinal fluid (SIF)	1089:1120	After incubation in simulated intestinal fluid (SIF), the gel containing alginate only swelled while gels containing untreated MPC and pre-heated MPC experienced degradation and a severe mass loss.
34865793	6	71	dep	reduced	944:950	arg1	%					960:960	16.4%	956:960	16.4%	956:960	Similarly, the addition of cold-renneted MPC reduced (by 16.4%) the degree of syneresis of the alginate composite gels after incubation in simulated gastric fluid (SGF).
34865793	7	72	theme	gel	1127:1129	arg1	alginate					1142:1149	the gel containing alginate	1123:1149	the gel containing alginate	1123:1149	After incubation in simulated intestinal fluid (SIF), the gel containing alginate only swelled while gels containing untreated MPC and pre-heated MPC experienced degradation and a severe mass loss.
34865793	5	73	from	degree	851:856	arg1	gels					884:887	composite gels	874:887	composite gels	874:887	The addition of cold-renneted MPC reduced the degree of syneresis in composite gels by 13.7%.
34865793	6	74	theme	alginate	994:1001	arg1	gels					1013:1016	the alginate composite gels	990:1016	the alginate composite gels	990:1016	Similarly, the addition of cold-renneted MPC reduced (by 16.4%) the degree of syneresis of the alginate composite gels after incubation in simulated gastric fluid (SGF).
34865793	7	75	theme	intestinal	1099:1108	arg1	SIF					1117:1119	SIF	1117:1119	SIF	1117:1119	After incubation in simulated intestinal fluid (SIF), the gel containing alginate only swelled while gels containing untreated MPC and pre-heated MPC experienced degradation and a severe mass loss.
34865793	7	75	theme	intestinal	1099:1108	arg1	fluid					1110:1114	simulated intestinal fluid	1089:1114	simulated intestinal fluid (SIF)	1089:1120	After incubation in simulated intestinal fluid (SIF), the gel containing alginate only swelled while gels containing untreated MPC and pre-heated MPC experienced degradation and a severe mass loss.
34865793	10	76	theme	induced	1556:1562	arg1	method					1573:1578	cold-rennet induced gelation method	1544:1578	cold-rennet induced gelation method	1544:1578	Therefore, based on these results, it is indicated that cold-rennet induced gelation method could increase the effectiveness of MPC as a composite material for alginate gels.
34865793	1	77	dep	concentrate	180:190	arg1	cold-renneted					212:224	cold-renneted	212:224	cold-renneted	212:224	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	1	77	dep	concentrate	180:190	arg1	pre-heated					231:240	pre-heated	231:240	pre-heated	231:240	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	1	77	dep	concentrate	180:190	arg1	untreated					201:209	untreated	201:209	untreated	201:209	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	9	78	theme	cold-renneted	1368:1380	arg1	gels					1396:1399	only cold-renneted MPC composite gels	1363:1399	only cold-renneted MPC composite gels	1363:1399	Moreover, only cold-renneted MPC composite gels showed lower shrinkage and wrinkles on the surface of the beads during lyophilization.
34865793	3	79	theme	alginate	709:716	arg1	gels					718:721	the alginate gels	705:721	the alginate gels	705:721	The results showed that the addition of untreated and treated MPCs reduced the hardness, the stiffness and the solid-like behavior of the alginate gels.
34865793	1	80	theme	physical/mechanical	275:293	arg1	properties					295:304	the physical/mechanical properties	271:304	the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms	271:404	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	3	81	theme	gels	718:721	arg1	behavior					693:700	the solid-like behavior	678:700	the solid-like behavior of the alginate gels	678:721	The results showed that the addition of untreated and treated MPCs reduced the hardness, the stiffness and the solid-like behavior of the alginate gels.
34865793	3	81	theme	gels	718:721	arg1	hardness					650:657	the hardness	646:657	the hardness	646:657	The results showed that the addition of untreated and treated MPCs reduced the hardness, the stiffness and the solid-like behavior of the alginate gels.
34865793	3	81	theme	gels	718:721	arg1	stiffness					664:672	the stiffness	660:672	the stiffness	660:672	The results showed that the addition of untreated and treated MPCs reduced the hardness, the stiffness and the solid-like behavior of the alginate gels.
34865793	1	82	from	effects	156:162	arg1	properties					295:304	the physical/mechanical properties	271:304	the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms	271:404	This study investigated the effects of milk protein concentrate (MPC85) (untreated, cold-renneted, and pre-heated (80 °C, 30 min)) addition on the physical/mechanical properties of sodium alginate (2% w/w) composite gels in millimeter-size (bead) and centrimeter-size gel forms.
34865793	6	83	theme	syneresis	977:985	arg1	degree					967:972	the degree	963:972	the degree of syneresis of the alginate composite gels	963:1016	Similarly, the addition of cold-renneted MPC reduced (by 16.4%) the degree of syneresis of the alginate composite gels after incubation in simulated gastric fluid (SGF).
34865793	2	84	theme	syneresis	453:461	arg1	degree					443:448	the degree	439:448	the degree of syneresis	439:461	The gels were characterized for the degree of syneresis, swelling behavior, hardness, stiffness, viscoelastic behavior, and surface morphology of freeze-dried gel.
33058985	5	0	theme	gene	703:706	arg1	cluster					708:714	the gene cluster	699:714	the gene cluster	699:714	Functions of the proteins encoded in the gene cluster, including the enzymes involved in the O-polysaccharide biosynthesis and glycosyl transferases, were putatively assigned by comparison with available database sequences.
33058985	6	1	from	A-6-5	979:983	arg1	uranyl					931:936	uranyl	931:936	uranyl	931:936	Formation of a new coordination bond between uranyl and the O-polysaccharide from P. veronii A-6-5 was demonstrated using FTIR spectroscopy; it may affect uranyl migration in the groundwaters due to its immobilization on microbial biofilms.
33058985	6	1	from	A-6-5	979:983	arg1	O-polysaccharide					946:961	the O-polysaccharide	942:961	the O-polysaccharide from P. veronii A-6-5	942:983	Formation of a new coordination bond between uranyl and the O-polysaccharide from P. veronii A-6-5 was demonstrated using FTIR spectroscopy; it may affect uranyl migration in the groundwaters due to its immobilization on microbial biofilms.
33058985	3	2	theme	following	475:483	arg1	structure					485:493	the following structure	471:493	the following structure: [Formula: see text]	471:514	The trisaccharide O-repeating unit was found to have the following structure: [Formula: see text] [Formula: see text] where Hb is 3-hydroxybutanoyl.
33058985	6	3	theme	P.	968:969	arg1	A-6-5					979:983	P. veronii A-6-5	968:983	P. veronii A-6-5	968:983	Formation of a new coordination bond between uranyl and the O-polysaccharide from P. veronii A-6-5 was demonstrated using FTIR spectroscopy; it may affect uranyl migration in the groundwaters due to its immobilization on microbial biofilms.
33058985	4	4	theme	gene	633:636	arg1	cluster					638:644	a respective OPS gene cluster	616:644	a respective OPS gene cluster	616:644	The genome of P. veronii A-6-5 was sequenced and a respective OPS gene cluster was identified.
33058985	8	5	theme	sludge	1514:1519	arg1	treatment					1454:1462	treatment	1454:1462	treatment of uranium-contaminated groundwater and activated sludge	1454:1519	The data obtained are of importance for development of the biotechnologies for treatment of uranium-contaminated groundwater and activated sludge.
33058985	0	6	theme	uranium	90:96	arg1	bonding					98:104	its uranium bonding	86:104	its uranium bonding	86:104	Structure and gene cluster of the O-polysaccharide from Pseudomonas veronii A-6-5 and its uranium bonding.
33058985	7	7	theme	uranyl	1347:1352	arg1	ion					1354:1356	the uranyl ion	1343:1356	the uranyl ion	1343:1356	Applied importance of this work is that the structure of the O-polysaccharide of a strain isolated from uranium-contaminated groundwater was determined and the character of interaction between the polysaccharide and the uranyl ion was established.
33058985	2	8	theme	acid	279:282	arg1	degradation					284:294	mild acid degradation	274:294	mild acid degradation of the lipopolysaccharide of P. veronii A-6-5	274:340	The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of P. veronii A-6-5 and studied using sugar analysis and 1D and 2D 1H and 13C NMR spectroscopy.
33058985	5	9	with	comparison	840:849	arg1	sequences					875:883	available database sequences	856:883	available database sequences	856:883	Functions of the proteins encoded in the gene cluster, including the enzymes involved in the O-polysaccharide biosynthesis and glycosyl transferases, were putatively assigned by comparison with available database sequences.
33058985	2	10	theme	mild	274:277	arg1	degradation					284:294	mild acid degradation	274:294	mild acid degradation of the lipopolysaccharide of P. veronii A-6-5	274:340	The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of P. veronii A-6-5 and studied using sugar analysis and 1D and 2D 1H and 13C NMR spectroscopy.
33058985	4	11	theme	A-6-5	592:596	arg1	genome					571:576	The genome	567:576	The genome of P. veronii A-6-5	567:596	The genome of P. veronii A-6-5 was sequenced and a respective OPS gene cluster was identified.
33058985	5	12	theme	proteins	679:686	arg1	transferases					798:809	glycosyl transferases	789:809	glycosyl transferases	789:809	Functions of the proteins encoded in the gene cluster, including the enzymes involved in the O-polysaccharide biosynthesis and glycosyl transferases, were putatively assigned by comparison with available database sequences.
33058985	5	12	theme	proteins	679:686	arg1	enzymes					731:737	the enzymes	727:737	the enzymes involved in the O-polysaccharide biosynthesis	727:783	Functions of the proteins encoded in the gene cluster, including the enzymes involved in the O-polysaccharide biosynthesis and glycosyl transferases, were putatively assigned by comparison with available database sequences.
33058985	5	12	theme	proteins	679:686	arg1	Functions					662:670	Functions	662:670	Functions	662:670	Functions of the proteins encoded in the gene cluster, including the enzymes involved in the O-polysaccharide biosynthesis and glycosyl transferases, were putatively assigned by comparison with available database sequences.
33058985	5	13	theme	database	866:873	arg1	sequences					875:883	available database sequences	856:883	available database sequences	856:883	Functions of the proteins encoded in the gene cluster, including the enzymes involved in the O-polysaccharide biosynthesis and glycosyl transferases, were putatively assigned by comparison with available database sequences.
33058985	6	14	theme	uranyl	1041:1046	arg1	migration					1048:1056	uranyl migration	1041:1056	uranyl migration	1041:1056	Formation of a new coordination bond between uranyl and the O-polysaccharide from P. veronii A-6-5 was demonstrated using FTIR spectroscopy; it may affect uranyl migration in the groundwaters due to its immobilization on microbial biofilms.
33058985	1	15	attach	isolated	162:169	arg1	aquifer					183:189	a deep aquifer	176:189	a deep aquifer contaminated with nitrates and uranium	176:228	A denitrifying bacterium Pseudomonas veronii A-6-5 was isolated from a deep aquifer contaminated with nitrates and uranium.
33058985	1	15	attach	isolated	162:169	arg2	bacterium					122:130	A denitrifying bacterium	107:130	A denitrifying bacterium Pseudomonas veronii A-6-5	107:156	A denitrifying bacterium Pseudomonas veronii A-6-5 was isolated from a deep aquifer contaminated with nitrates and uranium.
33058985	1	16	dep	bacterium	122:130	arg1	A-6-5					152:156	Pseudomonas veronii A-6-5	132:156	A denitrifying bacterium Pseudomonas veronii A-6-5	107:156	A denitrifying bacterium Pseudomonas veronii A-6-5 was isolated from a deep aquifer contaminated with nitrates and uranium.
33058985	3	17	dep	see	506:508	arg1	[Formula					496:503	[Formula	496:503	[Formula	496:503	The trisaccharide O-repeating unit was found to have the following structure: [Formula: see text] [Formula: see text] where Hb is 3-hydroxybutanoyl.
33058985	6	18	theme	bond	918:921	arg1	Formation					886:894	Formation	886:894	Formation of a new coordination bond between uranyl and the O-polysaccharide from P. veronii A-6-5	886:983	Formation of a new coordination bond between uranyl and the O-polysaccharide from P. veronii A-6-5 was demonstrated using FTIR spectroscopy; it may affect uranyl migration in the groundwaters due to its immobilization on microbial biofilms.
33058985	7	19	theme	work	1154:1157	arg1	importance					1135:1144	Applied importance	1127:1144	Applied importance of this work	1127:1157	Applied importance of this work is that the structure of the O-polysaccharide of a strain isolated from uranium-contaminated groundwater was determined and the character of interaction between the polysaccharide and the uranyl ion was established.
33058985	3	20	dep	see	526:528	arg1	[Formula					516:523	[Formula	516:523	[Formula	516:523	The trisaccharide O-repeating unit was found to have the following structure: [Formula: see text] [Formula: see text] where Hb is 3-hydroxybutanoyl.
33058985	3	21	contain	have	466:469	arg2	structure					485:493	the following structure	471:493	the following structure: [Formula: see text]	471:514	The trisaccharide O-repeating unit was found to have the following structure: [Formula: see text] [Formula: see text] where Hb is 3-hydroxybutanoyl.
33058985	3	21	contain	have	466:469	arg1	unit					448:451	The trisaccharide O-repeating unit	418:451	The trisaccharide O-repeating unit	418:451	The trisaccharide O-repeating unit was found to have the following structure: [Formula: see text] [Formula: see text] where Hb is 3-hydroxybutanoyl.
33058985	2	22	theme	lipopolysaccharide	303:320	arg1	degradation					284:294	mild acid degradation	274:294	mild acid degradation of the lipopolysaccharide of P. veronii A-6-5	274:340	The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of P. veronii A-6-5 and studied using sugar analysis and 1D and 2D 1H and 13C NMR spectroscopy.
33058985	7	23	theme	uranium-contaminated	1231:1250	arg1	groundwater					1252:1262	uranium-contaminated groundwater	1231:1262	uranium-contaminated groundwater	1231:1262	Applied importance of this work is that the structure of the O-polysaccharide of a strain isolated from uranium-contaminated groundwater was determined and the character of interaction between the polysaccharide and the uranyl ion was established.
33058985	1	24	theme	deep	178:181	arg1	aquifer					183:189	a deep aquifer	176:189	a deep aquifer contaminated with nitrates and uranium	176:228	A denitrifying bacterium Pseudomonas veronii A-6-5 was isolated from a deep aquifer contaminated with nitrates and uranium.
33058985	8	25	theme	uranium-contaminated	1467:1486	arg1	groundwater					1488:1498	uranium-contaminated groundwater	1467:1498	uranium-contaminated groundwater	1467:1498	The data obtained are of importance for development of the biotechnologies for treatment of uranium-contaminated groundwater and activated sludge.
33058985	3	26	dep	structure	485:493	arg1	see					506:508	see	506:508	see text	506:513	The trisaccharide O-repeating unit was found to have the following structure: [Formula: see text] [Formula: see text] where Hb is 3-hydroxybutanoyl.
33058985	5	27	theme	available	856:864	arg1	sequences					875:883	available database sequences	856:883	available database sequences	856:883	Functions of the proteins encoded in the gene cluster, including the enzymes involved in the O-polysaccharide biosynthesis and glycosyl transferases, were putatively assigned by comparison with available database sequences.
33058985	5	28	theme	glycosyl	789:796	arg1	transferases					798:809	glycosyl transferases	789:809	glycosyl transferases	789:809	Functions of the proteins encoded in the gene cluster, including the enzymes involved in the O-polysaccharide biosynthesis and glycosyl transferases, were putatively assigned by comparison with available database sequences.
33058985	8	29	theme	biotechnologies	1434:1448	arg1	development					1415:1425	development	1415:1425	development of the biotechnologies for treatment of uranium-contaminated groundwater and activated sludge	1415:1519	The data obtained are of importance for development of the biotechnologies for treatment of uranium-contaminated groundwater and activated sludge.
33058985	7	30	theme	strain	1210:1215	arg1	O-polysaccharide					1188:1203	the O-polysaccharide	1184:1203	the O-polysaccharide of a strain isolated from uranium-contaminated groundwater	1184:1262	Applied importance of this work is that the structure of the O-polysaccharide of a strain isolated from uranium-contaminated groundwater was determined and the character of interaction between the polysaccharide and the uranyl ion was established.
33058985	6	31	theme	FTIR	1008:1011	arg1	spectroscopy					1013:1024	FTIR spectroscopy	1008:1024	FTIR spectroscopy	1008:1024	Formation of a new coordination bond between uranyl and the O-polysaccharide from P. veronii A-6-5 was demonstrated using FTIR spectroscopy; it may affect uranyl migration in the groundwaters due to its immobilization on microbial biofilms.
33058985	4	32	theme	P.	581:582	arg1	A-6-5					592:596	P. veronii A-6-5	581:596	P. veronii A-6-5	581:596	The genome of P. veronii A-6-5 was sequenced and a respective OPS gene cluster was identified.
33058985	2	33	theme	NMR	400:402	arg1	spectroscopy					404:415	NMR spectroscopy	400:415	NMR spectroscopy	400:415	The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of P. veronii A-6-5 and studied using sugar analysis and 1D and 2D 1H and 13C NMR spectroscopy.
33058985	0	34	dep	Pseudomonas	56:66	arg1	veronii					68:74	veronii	68:74	veronii	68:74	Structure and gene cluster of the O-polysaccharide from Pseudomonas veronii A-6-5 and its uranium bonding.
33058985	2	35	theme	sugar	360:364	arg1	analysis					366:373	sugar analysis	360:373	sugar analysis	360:373	The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of P. veronii A-6-5 and studied using sugar analysis and 1D and 2D 1H and 13C NMR spectroscopy.
33058985	2	36	theme	veronii	328:334	arg1	A-6-5					336:340	P. veronii A-6-5	325:340	P. veronii A-6-5	325:340	The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of P. veronii A-6-5 and studied using sugar analysis and 1D and 2D 1H and 13C NMR spectroscopy.
33058985	0	37	from	A-6-5	76:80	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and gene cluster of the O-polysaccharide from Pseudomonas veronii A-6-5 and its uranium bonding.
33058985	0	37	from	A-6-5	76:80	arg1	cluster					19:25	gene cluster	14:25	gene cluster	14:25	Structure and gene cluster of the O-polysaccharide from Pseudomonas veronii A-6-5 and its uranium bonding.
33058985	2	38	dep	1H	389:390	arg1	spectroscopy					404:415	NMR spectroscopy	400:415	NMR spectroscopy	400:415	The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of P. veronii A-6-5 and studied using sugar analysis and 1D and 2D 1H and 13C NMR spectroscopy.
33058985	7	39	theme	interaction	1300:1310	arg1	character					1287:1295	the character	1283:1295	the character of interaction between the polysaccharide and the uranyl ion	1283:1356	Applied importance of this work is that the structure of the O-polysaccharide of a strain isolated from uranium-contaminated groundwater was determined and the character of interaction between the polysaccharide and the uranyl ion was established.
33058985	3	40	theme	trisaccharide	422:434	arg1	unit					448:451	The trisaccharide O-repeating unit	418:451	The trisaccharide O-repeating unit	418:451	The trisaccharide O-repeating unit was found to have the following structure: [Formula: see text] [Formula: see text] where Hb is 3-hydroxybutanoyl.
33058985	4	41	theme	OPS	629:631	arg1	cluster					638:644	a respective OPS gene cluster	616:644	a respective OPS gene cluster	616:644	The genome of P. veronii A-6-5 was sequenced and a respective OPS gene cluster was identified.
33058985	6	42	theme	coordination	905:916	arg1	bond					918:921	a new coordination bond	899:921	a new coordination bond between uranyl and the O-polysaccharide from P. veronii A-6-5	899:983	Formation of a new coordination bond between uranyl and the O-polysaccharide from P. veronii A-6-5 was demonstrated using FTIR spectroscopy; it may affect uranyl migration in the groundwaters due to its immobilization on microbial biofilms.
33058985	7	43	theme	Applied	1127:1133	arg1	importance					1135:1144	Applied importance	1127:1144	Applied importance of this work	1127:1157	Applied importance of this work is that the structure of the O-polysaccharide of a strain isolated from uranium-contaminated groundwater was determined and the character of interaction between the polysaccharide and the uranyl ion was established.
33058985	3	44	theme	O-repeating	436:446	arg1	unit					448:451	The trisaccharide O-repeating unit	418:451	The trisaccharide O-repeating unit	418:451	The trisaccharide O-repeating unit was found to have the following structure: [Formula: see text] [Formula: see text] where Hb is 3-hydroxybutanoyl.
33058985	6	45	theme	new	901:903	arg1	bond					918:921	a new coordination bond	899:921	a new coordination bond between uranyl and the O-polysaccharide from P. veronii A-6-5	899:983	Formation of a new coordination bond between uranyl and the O-polysaccharide from P. veronii A-6-5 was demonstrated using FTIR spectroscopy; it may affect uranyl migration in the groundwaters due to its immobilization on microbial biofilms.
33058985	5	46	theme	O-polysaccharide	755:770	arg1	biosynthesis					772:783	the O-polysaccharide biosynthesis	751:783	the O-polysaccharide biosynthesis	751:783	Functions of the proteins encoded in the gene cluster, including the enzymes involved in the O-polysaccharide biosynthesis and glycosyl transferases, were putatively assigned by comparison with available database sequences.
33058985	7	47	theme	O-polysaccharide	1188:1203	arg1	structure					1171:1179	the structure	1167:1179	the structure of the O-polysaccharide of a strain isolated from uranium-contaminated groundwater	1167:1262	Applied importance of this work is that the structure of the O-polysaccharide of a strain isolated from uranium-contaminated groundwater was determined and the character of interaction between the polysaccharide and the uranyl ion was established.
33058985	2	48	theme	A-6-5	336:340	arg1	lipopolysaccharide					303:320	the lipopolysaccharide	299:320	the lipopolysaccharide of P. veronii A-6-5	299:340	The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of P. veronii A-6-5 and studied using sugar analysis and 1D and 2D 1H and 13C NMR spectroscopy.
33058985	0	49	theme	O-polysaccharide	34:49	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and gene cluster of the O-polysaccharide from Pseudomonas veronii A-6-5 and its uranium bonding.
33058985	0	49	theme	O-polysaccharide	34:49	arg1	cluster					19:25	gene cluster	14:25	gene cluster	14:25	Structure and gene cluster of the O-polysaccharide from Pseudomonas veronii A-6-5 and its uranium bonding.
33058985	8	50	theme	activated	1504:1512	arg1	sludge					1514:1519	activated sludge	1504:1519	activated sludge	1504:1519	The data obtained are of importance for development of the biotechnologies for treatment of uranium-contaminated groundwater and activated sludge.
33058985	6	51	theme	microbial	1107:1115	arg1	biofilms					1117:1124	microbial biofilms	1107:1124	microbial biofilms	1107:1124	Formation of a new coordination bond between uranyl and the O-polysaccharide from P. veronii A-6-5 was demonstrated using FTIR spectroscopy; it may affect uranyl migration in the groundwaters due to its immobilization on microbial biofilms.
33058985	6	52	from	immobilization	1089:1102	arg1	biofilms					1117:1124	microbial biofilms	1107:1124	microbial biofilms	1107:1124	Formation of a new coordination bond between uranyl and the O-polysaccharide from P. veronii A-6-5 was demonstrated using FTIR spectroscopy; it may affect uranyl migration in the groundwaters due to its immobilization on microbial biofilms.
33058985	1	53	theme	denitrifying	109:120	arg1	bacterium					122:130	A denitrifying bacterium	107:130	A denitrifying bacterium Pseudomonas veronii A-6-5	107:156	A denitrifying bacterium Pseudomonas veronii A-6-5 was isolated from a deep aquifer contaminated with nitrates and uranium.
33058985	0	54	from	bonding	98:104	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and gene cluster of the O-polysaccharide from Pseudomonas veronii A-6-5 and its uranium bonding.
33058985	0	54	from	bonding	98:104	arg1	cluster					19:25	gene cluster	14:25	gene cluster	14:25	Structure and gene cluster of the O-polysaccharide from Pseudomonas veronii A-6-5 and its uranium bonding.
33058985	2	55	theme	P.	325:326	arg1	A-6-5					336:340	P. veronii A-6-5	325:340	P. veronii A-6-5	325:340	The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of P. veronii A-6-5 and studied using sugar analysis and 1D and 2D 1H and 13C NMR spectroscopy.
33058985	6	56	theme	due	1078:1080	arg1	groundwaters					1065:1076	the groundwaters	1061:1076	the groundwaters due to its immobilization on microbial biofilms	1061:1124	Formation of a new coordination bond between uranyl and the O-polysaccharide from P. veronii A-6-5 was demonstrated using FTIR spectroscopy; it may affect uranyl migration in the groundwaters due to its immobilization on microbial biofilms.
33058985	8	57	theme	groundwater	1488:1498	arg1	treatment					1454:1462	treatment	1454:1462	treatment of uranium-contaminated groundwater and activated sludge	1454:1519	The data obtained are of importance for development of the biotechnologies for treatment of uranium-contaminated groundwater and activated sludge.
33058985	2	58	dep	analysis	366:373	arg1	1H					389:390	1H	389:390	1H	389:390	The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of P. veronii A-6-5 and studied using sugar analysis and 1D and 2D 1H and 13C NMR spectroscopy.
33058985	2	58	dep	analysis	366:373	arg1	13C					396:398	13C	396:398	13C	396:398	The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of P. veronii A-6-5 and studied using sugar analysis and 1D and 2D 1H and 13C NMR spectroscopy.
33058985	1	59	theme	Pseudomonas	132:142	arg1	A-6-5					152:156	Pseudomonas veronii A-6-5	132:156	A denitrifying bacterium Pseudomonas veronii A-6-5	107:156	A denitrifying bacterium Pseudomonas veronii A-6-5 was isolated from a deep aquifer contaminated with nitrates and uranium.
33058985	4	60	theme	veronii	584:590	arg1	A-6-5					592:596	P. veronii A-6-5	581:596	P. veronii A-6-5	581:596	The genome of P. veronii A-6-5 was sequenced and a respective OPS gene cluster was identified.
33058985	1	61	theme	veronii	144:150	arg1	A-6-5					152:156	Pseudomonas veronii A-6-5	132:156	A denitrifying bacterium Pseudomonas veronii A-6-5	107:156	A denitrifying bacterium Pseudomonas veronii A-6-5 was isolated from a deep aquifer contaminated with nitrates and uranium.
33058985	0	62	theme	Pseudomonas	56:66	arg1	A-6-5					76:80	Pseudomonas veronii A-6-5	56:80	Pseudomonas veronii A-6-5	56:80	Structure and gene cluster of the O-polysaccharide from Pseudomonas veronii A-6-5 and its uranium bonding.
33058985	7	63	attach	isolated	1217:1224	arg1	groundwater					1252:1262	uranium-contaminated groundwater	1231:1262	uranium-contaminated groundwater	1231:1262	Applied importance of this work is that the structure of the O-polysaccharide of a strain isolated from uranium-contaminated groundwater was determined and the character of interaction between the polysaccharide and the uranyl ion was established.
33058985	7	63	attach	isolated	1217:1224	arg2	strain					1210:1215	a strain	1208:1215	a strain isolated from uranium-contaminated groundwater	1208:1262	Applied importance of this work is that the structure of the O-polysaccharide of a strain isolated from uranium-contaminated groundwater was determined and the character of interaction between the polysaccharide and the uranyl ion was established.
33058985	0	64	theme	gene	14:17	arg1	cluster					19:25	gene cluster	14:25	gene cluster	14:25	Structure and gene cluster of the O-polysaccharide from Pseudomonas veronii A-6-5 and its uranium bonding.
33058985	4	65	theme	respective	618:627	arg1	cluster					638:644	a respective OPS gene cluster	616:644	a respective OPS gene cluster	616:644	The genome of P. veronii A-6-5 was sequenced and a respective OPS gene cluster was identified.
33058985	6	66	theme	veronii	971:977	arg1	A-6-5					979:983	P. veronii A-6-5	968:983	P. veronii A-6-5	968:983	Formation of a new coordination bond between uranyl and the O-polysaccharide from P. veronii A-6-5 was demonstrated using FTIR spectroscopy; it may affect uranyl migration in the groundwaters due to its immobilization on microbial biofilms.
35074120	4	0	theme	apparent	554:561	arg1	behavior					573:580	its apparent viscosity behavior	550:580	its apparent viscosity behavior	550:580	The correlation between the morphological and structural properties throughout its pasting profile explains its apparent viscosity behavior.
35074120	0	1	theme	pasting	74:80	arg1	profile					82:88	its pasting profile	70:88	its pasting profile	70:88	Influence of physicochemical changes of the avocado starch throughout its pasting profile: Combined extraction.
35074120	4	2	theme	morphological	470:482	arg1	properties					499:508	the morphological and structural properties	466:508	the morphological and structural properties throughout its pasting profile	466:539	The correlation between the morphological and structural properties throughout its pasting profile explains its apparent viscosity behavior.
35074120	1	3	from	effect	146:151	arg1	profile					189:195	the pasting profile	177:195	the pasting profile of isolated avocado starch (C-type) obtained by a combined mechanical and ultrasonic process	177:288	This work focused on studying the effect of lamellae presence on the pasting profile of isolated avocado starch (C-type) obtained by a combined mechanical and ultrasonic process.
35074120	7	4	theme	lamellae	874:881	arg1	presence					862:869	the presence	858:869	the presence of lamellae	858:881	After gelatinization, the presence of lamellae originated from the partial fragmentation of the crystals.
35074120	0	5	theme	Combined	91:98	arg1	extraction					100:109	Combined extraction	91:109	Combined extraction	91:109	Influence of physicochemical changes of the avocado starch throughout its pasting profile: Combined extraction.
35074120	6	6	theme	amylose-lipid	799:811	arg1	nanocrystals					772:783	the nanocrystals solvation and amylose-lipid complex	768:819	the nanocrystals solvation and amylose-lipid complex	768:819	DSC reveals endothermal transitions at 68 °C and 119 °C associated with the nanocrystals solvation and amylose-lipid complex, respectively.
35074120	6	6	theme	amylose-lipid	799:811	arg1	complex					813:819	amylose-lipid complex	799:819	amylose-lipid complex	799:819	DSC reveals endothermal transitions at 68 °C and 119 °C associated with the nanocrystals solvation and amylose-lipid complex, respectively.
35074120	0	7	dep	extraction	100:109	arg1	Influence					0:8	Influence	0:8	Influence of physicochemical changes of the avocado starch throughout its pasting profile	0:88	Influence of physicochemical changes of the avocado starch throughout its pasting profile: Combined extraction.
35074120	4	8	theme	viscosity	563:571	arg1	behavior					573:580	its apparent viscosity behavior	550:580	its apparent viscosity behavior	550:580	The correlation between the morphological and structural properties throughout its pasting profile explains its apparent viscosity behavior.
35074120	5	9	theme	non-conventional	602:617	arg1	morphology					629:638	a non-conventional irregular morphology	600:638	a non-conventional irregular morphology with sizes ranging from 35 to 40 μm in their long side	600:693	Granules exhibit a non-conventional irregular morphology with sizes ranging from 35 to 40 μm in their long side.
35074120	9	10	theme	pasting	1091:1097	arg1	behaviors					1099:1107	the pasting behaviors	1087:1107	the pasting behaviors in any starch	1087:1121	This correlation could be considered a new methodology to understand the pasting behaviors in any starch.
35074120	1	11	theme	lamellae	156:163	arg1	presence					165:172	lamellae presence	156:172	lamellae presence	156:172	This work focused on studying the effect of lamellae presence on the pasting profile of isolated avocado starch (C-type) obtained by a combined mechanical and ultrasonic process.
35074120	5	12	dep	40 μm	670:674	arg1	to					667:668	to	667:668	to	667:668	Granules exhibit a non-conventional irregular morphology with sizes ranging from 35 to 40 μm in their long side.
35074120	5	13	theme	irregular	619:627	arg1	morphology					629:638	a non-conventional irregular morphology	600:638	a non-conventional irregular morphology with sizes ranging from 35 to 40 μm in their long side	600:693	Granules exhibit a non-conventional irregular morphology with sizes ranging from 35 to 40 μm in their long side.
35074120	1	14	theme	presence	165:172	arg1	effect					146:151	the effect	142:151	the effect of lamellae presence on the pasting profile of isolated avocado starch (C-type) obtained by a combined mechanical and ultrasonic process	142:288	This work focused on studying the effect of lamellae presence on the pasting profile of isolated avocado starch (C-type) obtained by a combined mechanical and ultrasonic process.
35074120	4	15	theme	pasting	525:531	arg1	profile					533:539	its pasting profile	521:539	its pasting profile	521:539	The correlation between the morphological and structural properties throughout its pasting profile explains its apparent viscosity behavior.
35074120	6	16	dep	nanocrystals	772:783	arg1	nanocrystals					772:783	the nanocrystals solvation and amylose-lipid complex	768:819	the nanocrystals solvation and amylose-lipid complex	768:819	DSC reveals endothermal transitions at 68 °C and 119 °C associated with the nanocrystals solvation and amylose-lipid complex, respectively.
35074120	6	16	dep	nanocrystals	772:783	arg1	solvation					785:793	solvation	785:793	solvation	785:793	DSC reveals endothermal transitions at 68 °C and 119 °C associated with the nanocrystals solvation and amylose-lipid complex, respectively.
35074120	6	16	dep	nanocrystals	772:783	arg1	complex					813:819	amylose-lipid complex	799:819	amylose-lipid complex	799:819	DSC reveals endothermal transitions at 68 °C and 119 °C associated with the nanocrystals solvation and amylose-lipid complex, respectively.
35074120	1	17	theme	combined	247:254	arg1	process					282:288	a combined mechanical and ultrasonic process	245:288	a combined mechanical and ultrasonic process	245:288	This work focused on studying the effect of lamellae presence on the pasting profile of isolated avocado starch (C-type) obtained by a combined mechanical and ultrasonic process.
35074120	7	18	theme	crystals	932:939	arg1	fragmentation					911:923	the partial fragmentation	899:923	the partial fragmentation of the crystals	899:939	After gelatinization, the presence of lamellae originated from the partial fragmentation of the crystals.
35074120	0	19	theme	changes	29:35	arg1	Influence					0:8	Influence	0:8	Influence of physicochemical changes of the avocado starch throughout its pasting profile	0:88	Influence of physicochemical changes of the avocado starch throughout its pasting profile: Combined extraction.
35074120	1	20	theme	mechanical	256:265	arg1	process					282:288	a combined mechanical and ultrasonic process	245:288	a combined mechanical and ultrasonic process	245:288	This work focused on studying the effect of lamellae presence on the pasting profile of isolated avocado starch (C-type) obtained by a combined mechanical and ultrasonic process.
35074120	5	21	theme	long	685:688	arg1	side					690:693	their long side	679:693	their long side	679:693	Granules exhibit a non-conventional irregular morphology with sizes ranging from 35 to 40 μm in their long side.
35074120	2	22	from	rich	347:350	arg1	K					358:358	K	358:358	K	358:358	Inductively Couple Plasma indicates that this starch is rich in P, K, Na, and Ca.
35074120	2	22	from	rich	347:350	arg1	P					355:355	P	355:355	P	355:355	Inductively Couple Plasma indicates that this starch is rich in P, K, Na, and Ca.
35074120	2	22	from	rich	347:350	arg1	Na					361:362	Na	361:362	Na	361:362	Inductively Couple Plasma indicates that this starch is rich in P, K, Na, and Ca.
35074120	2	22	from	rich	347:350	arg1	Ca.					369:371	Ca.	369:371	Ca.	369:371	Inductively Couple Plasma indicates that this starch is rich in P, K, Na, and Ca.
35074120	1	23	theme	pasting	181:187	arg1	profile					189:195	the pasting profile	177:195	the pasting profile of isolated avocado starch (C-type) obtained by a combined mechanical and ultrasonic process	177:288	This work focused on studying the effect of lamellae presence on the pasting profile of isolated avocado starch (C-type) obtained by a combined mechanical and ultrasonic process.
35074120	0	24	theme	physicochemical	13:27	arg1	changes					29:35	physicochemical changes	13:35	physicochemical changes of the avocado starch	13:57	Influence of physicochemical changes of the avocado starch throughout its pasting profile: Combined extraction.
35074120	1	25	theme	ultrasonic	271:280	arg1	process					282:288	a combined mechanical and ultrasonic process	245:288	a combined mechanical and ultrasonic process	245:288	This work focused on studying the effect of lamellae presence on the pasting profile of isolated avocado starch (C-type) obtained by a combined mechanical and ultrasonic process.
35074120	3	26	theme	most	398:401	arg1	structure					415:423	the most crystalline structure	394:423	the most crystalline structure reported so far	394:439	This starch exhibits the most crystalline structure reported so far.
35074120	4	27	theme	structural	488:497	arg1	properties					499:508	the morphological and structural properties	466:508	the morphological and structural properties throughout its pasting profile	466:539	The correlation between the morphological and structural properties throughout its pasting profile explains its apparent viscosity behavior.
35074120	1	28	theme	isolated	200:207	arg1	starch					217:222	isolated avocado starch	200:222	isolated avocado starch (C-type) obtained by a combined mechanical and ultrasonic process	200:288	This work focused on studying the effect of lamellae presence on the pasting profile of isolated avocado starch (C-type) obtained by a combined mechanical and ultrasonic process.
35074120	0	29	theme	starch	52:57	arg1	changes					29:35	physicochemical changes	13:35	physicochemical changes of the avocado starch	13:57	Influence of physicochemical changes of the avocado starch throughout its pasting profile: Combined extraction.
35074120	1	30	dep	starch	217:222	arg1	C-type					225:230	C-type	225:230	C-type	225:230	This work focused on studying the effect of lamellae presence on the pasting profile of isolated avocado starch (C-type) obtained by a combined mechanical and ultrasonic process.
35074120	2	31	from	P	355:355	arg1	rich					347:350	rich	347:350	rich	347:350	Inductively Couple Plasma indicates that this starch is rich in P, K, Na, and Ca.
35074120	5	32	with	morphology	629:638	arg1	sizes					645:649	sizes	645:649	sizes ranging from 35 to 40 μm in their long side	645:693	Granules exhibit a non-conventional irregular morphology with sizes ranging from 35 to 40 μm in their long side.
35074120	0	33	theme	avocado	44:50	arg1	starch					52:57	the avocado starch	40:57	the avocado starch	40:57	Influence of physicochemical changes of the avocado starch throughout its pasting profile: Combined extraction.
35074120	8	34	theme	pasting	946:952	arg1	end					954:956	The pasting end	942:956	The pasting end	942:956	The pasting end exhibited a combined behavior between custard and hydrogel.
35074120	8	35	theme	combined	970:977	arg1	behavior					979:986	a combined behavior	968:986	a combined behavior between custard and hydrogel	968:1015	The pasting end exhibited a combined behavior between custard and hydrogel.
35074120	9	36	from	behaviors	1099:1107	arg1	starch					1116:1121	any starch	1112:1121	any starch	1112:1121	This correlation could be considered a new methodology to understand the pasting behaviors in any starch.
35074120	2	37	from	K	358:358	arg1	rich					347:350	rich	347:350	rich	347:350	Inductively Couple Plasma indicates that this starch is rich in P, K, Na, and Ca.
35074120	6	38	theme	endothermal	708:718	arg1	transitions					720:730	endothermal transitions	708:730	endothermal transitions	708:730	DSC reveals endothermal transitions at 68 °C and 119 °C associated with the nanocrystals solvation and amylose-lipid complex, respectively.
35074120	1	39	theme	avocado	209:215	arg1	starch					217:222	isolated avocado starch	200:222	isolated avocado starch (C-type) obtained by a combined mechanical and ultrasonic process	200:288	This work focused on studying the effect of lamellae presence on the pasting profile of isolated avocado starch (C-type) obtained by a combined mechanical and ultrasonic process.
35074120	7	40	theme	partial	903:909	arg1	fragmentation					911:923	the partial fragmentation	899:923	the partial fragmentation of the crystals	899:939	After gelatinization, the presence of lamellae originated from the partial fragmentation of the crystals.
35074120	1	41	theme	starch	217:222	arg1	profile					189:195	the pasting profile	177:195	the pasting profile of isolated avocado starch (C-type) obtained by a combined mechanical and ultrasonic process	177:288	This work focused on studying the effect of lamellae presence on the pasting profile of isolated avocado starch (C-type) obtained by a combined mechanical and ultrasonic process.
35074120	3	42	theme	crystalline	403:413	arg1	structure					415:423	the most crystalline structure	394:423	the most crystalline structure reported so far	394:439	This starch exhibits the most crystalline structure reported so far.
35074120	2	43	from	Na	361:362	arg1	rich					347:350	rich	347:350	rich	347:350	Inductively Couple Plasma indicates that this starch is rich in P, K, Na, and Ca.
35074120	2	44	from	Ca.	369:371	arg1	rich					347:350	rich	347:350	rich	347:350	Inductively Couple Plasma indicates that this starch is rich in P, K, Na, and Ca.
35074120	9	45	theme	new	1057:1059	arg1	methodology					1061:1071	a new methodology	1055:1071	a new methodology to understand the pasting behaviors in any starch	1055:1121	This correlation could be considered a new methodology to understand the pasting behaviors in any starch.
32718657	0	0	theme	silica	132:137	arg1	nanoparticle					139:150	silica nanoparticle	132:150	silica nanoparticle	132:150	Toward high-performance nanofibrillated cellulose/aramid fibrid paper-based composites via polyethyleneimine-assisted decoration of silica nanoparticle onto aramid fibrid.
32718657	5	1	theme	-AF	946:948	arg1	composites					962:971	NFC/(PEI/SiO2)-AF paper-based composites	932:971	NFC/(PEI/SiO2)-AF paper-based composites	932:971	Consequently, NFC/(PEI/SiO2)-AF paper-based composites showed better tensile toughness (∼6 % elongation at break) and mechanical strength (∼36.28 MPa), in comparison with NFC/AF.
32718657	1	2	theme	crucial	227:233	arg1	importance					235:244	crucial importance	227:244	crucial importance	227:244	Flexible paper-based nanocomposites dielectrics are of crucial importance in electrical insulation and advanced electrical power systems.
32718657	5	3	theme	NFC/	932:935	arg1	composites					962:971	NFC/(PEI/SiO2)-AF paper-based composites	932:971	NFC/(PEI/SiO2)-AF paper-based composites	932:971	Consequently, NFC/(PEI/SiO2)-AF paper-based composites showed better tensile toughness (∼6 % elongation at break) and mechanical strength (∼36.28 MPa), in comparison with NFC/AF.
32718657	4	4	theme	PEI/SiO2	803:810	arg1	AF					777:778	(PEI/SiO2)-modified AF	757:778	(PEI/SiO2)-modified AF (after crosslinking) ((PEI/SiO2)-AF)	757:815	It was found that composites prepared by NFC and (PEI/SiO2)-modified AF (after crosslinking) ((PEI/SiO2)-AF) showed dense structure, which was mainly due to enhanced interfacial interaction between AF and NFC.
32718657	4	4	theme	PEI/SiO2	803:810	arg1	-AF					812:814	(PEI/SiO2)-AF	802:814	(PEI/SiO2)-AF	802:814	It was found that composites prepared by NFC and (PEI/SiO2)-modified AF (after crosslinking) ((PEI/SiO2)-AF) showed dense structure, which was mainly due to enhanced interfacial interaction between AF and NFC.
32718657	0	5	theme	nanoparticle	139:150	arg1	decoration					118:127	polyethyleneimine-assisted decoration	91:127	polyethyleneimine-assisted decoration of silica nanoparticle onto aramid fibrid	91:169	Toward high-performance nanofibrillated cellulose/aramid fibrid paper-based composites via polyethyleneimine-assisted decoration of silica nanoparticle onto aramid fibrid.
32718657	5	6	theme	∼6	1006:1007	arg1	%					1009:1009	%	1009:1009	%	1009:1009	Consequently, NFC/(PEI/SiO2)-AF paper-based composites showed better tensile toughness (∼6 % elongation at break) and mechanical strength (∼36.28 MPa), in comparison with NFC/AF.
32718657	2	7	theme	fibrid	365:370	arg1	composite					381:389	a novel nanofibrillated cellulose/aramid fibrid (NFC/AF) composite	324:389	a novel nanofibrillated cellulose/aramid fibrid (NFC/AF) composite	324:389	In this work, a novel nanofibrillated cellulose/aramid fibrid (NFC/AF) composite was fabricated by vacuum-assisted filtration process.
32718657	6	8	theme	insulation	1130:1139	arg1	performance					1141:1151	the electrical insulation performance	1115:1151	the electrical insulation performance	1115:1151	More importantly, the electrical insulation performance and thermal stability of the composites were significantly improved.
32718657	4	9	theme	-modified	767:775	arg1	AF					777:778	(PEI/SiO2)-modified AF	757:778	(PEI/SiO2)-modified AF (after crosslinking) ((PEI/SiO2)-AF)	757:815	It was found that composites prepared by NFC and (PEI/SiO2)-modified AF (after crosslinking) ((PEI/SiO2)-AF) showed dense structure, which was mainly due to enhanced interfacial interaction between AF and NFC.
32718657	4	9	theme	-modified	767:775	arg1	-AF					812:814	(PEI/SiO2)-AF	802:814	(PEI/SiO2)-AF	802:814	It was found that composites prepared by NFC and (PEI/SiO2)-modified AF (after crosslinking) ((PEI/SiO2)-AF) showed dense structure, which was mainly due to enhanced interfacial interaction between AF and NFC.
32718657	3	10	theme	molecular	576:584	arg1	self-assembly					586:598	molecular self-assembly	576:598	molecular self-assembly with the aid of phosphoric acid (PA) pretreatment and subsequent polyethyleneimine (PEI) functionalization	576:705	In order to improve the dielectric property of the composites, carboxylated nano-SiO2 was chemically coated onto aramid fibrid via molecular self-assembly with the aid of phosphoric acid (PA) pretreatment and subsequent polyethyleneimine (PEI) functionalization.
32718657	5	11	theme	better	980:985	arg1	toughness					995:1003	better tensile toughness	980:1003	better tensile toughness (∼6 % elongation at break)	980:1030	Consequently, NFC/(PEI/SiO2)-AF paper-based composites showed better tensile toughness (∼6 % elongation at break) and mechanical strength (∼36.28 MPa), in comparison with NFC/AF.
32718657	3	12	theme	aramid	558:563	arg1	fibrid					565:570	aramid fibrid	558:570	aramid fibrid	558:570	In order to improve the dielectric property of the composites, carboxylated nano-SiO2 was chemically coated onto aramid fibrid via molecular self-assembly with the aid of phosphoric acid (PA) pretreatment and subsequent polyethyleneimine (PEI) functionalization.
32718657	2	13	theme	cellulose/aramid	348:363	arg1	composite					381:389	a novel nanofibrillated cellulose/aramid fibrid (NFC/AF) composite	324:389	a novel nanofibrillated cellulose/aramid fibrid (NFC/AF) composite	324:389	In this work, a novel nanofibrillated cellulose/aramid fibrid (NFC/AF) composite was fabricated by vacuum-assisted filtration process.
32718657	5	14	with	comparison	1073:1082	arg1	NFC/AF					1089:1094	NFC/AF	1089:1094	NFC/AF	1089:1094	Consequently, NFC/(PEI/SiO2)-AF paper-based composites showed better tensile toughness (∼6 % elongation at break) and mechanical strength (∼36.28 MPa), in comparison with NFC/AF.
32718657	0	15	theme	aramid	157:162	arg1	fibrid					164:169	aramid fibrid	157:169	aramid fibrid	157:169	Toward high-performance nanofibrillated cellulose/aramid fibrid paper-based composites via polyethyleneimine-assisted decoration of silica nanoparticle onto aramid fibrid.
32718657	5	16	theme	PEI/SiO2	937:944	arg1	composites					962:971	NFC/(PEI/SiO2)-AF paper-based composites	932:971	NFC/(PEI/SiO2)-AF paper-based composites	932:971	Consequently, NFC/(PEI/SiO2)-AF paper-based composites showed better tensile toughness (∼6 % elongation at break) and mechanical strength (∼36.28 MPa), in comparison with NFC/AF.
32718657	4	17	theme	interfacial	874:884	arg1	interaction					886:896	enhanced interfacial interaction	865:896	enhanced interfacial interaction between AF and NFC	865:915	It was found that composites prepared by NFC and (PEI/SiO2)-modified AF (after crosslinking) ((PEI/SiO2)-AF) showed dense structure, which was mainly due to enhanced interfacial interaction between AF and NFC.
32718657	2	18	theme	nanofibrillated	332:346	arg1	composite					381:389	a novel nanofibrillated cellulose/aramid fibrid (NFC/AF) composite	324:389	a novel nanofibrillated cellulose/aramid fibrid (NFC/AF) composite	324:389	In this work, a novel nanofibrillated cellulose/aramid fibrid (NFC/AF) composite was fabricated by vacuum-assisted filtration process.
32718657	0	19	theme	high-performance	7:22	arg1	composites					76:85	high-performance nanofibrillated cellulose/aramid fibrid paper-based composites	7:85	high-performance nanofibrillated cellulose/aramid fibrid paper-based composites via polyethyleneimine-assisted decoration of silica nanoparticle onto aramid fibrid	7:169	Toward high-performance nanofibrillated cellulose/aramid fibrid paper-based composites via polyethyleneimine-assisted decoration of silica nanoparticle onto aramid fibrid.
32718657	7	20	theme	electrical	1356:1365	arg1	applications					1378:1389	high-temperature electrical insulation applications	1339:1389	high-temperature electrical insulation applications	1339:1389	Accordingly, this work provides a facile approach to fabricate high-performance dielectric composites especially for high-temperature electrical insulation applications.
32718657	6	21	theme	composites	1182:1191	arg1	performance					1141:1151	the electrical insulation performance	1115:1151	the electrical insulation performance	1115:1151	More importantly, the electrical insulation performance and thermal stability of the composites were significantly improved.
32718657	6	21	theme	composites	1182:1191	arg1	stability					1165:1173	thermal stability	1157:1173	thermal stability	1157:1173	More importantly, the electrical insulation performance and thermal stability of the composites were significantly improved.
32718657	5	22	from	break	1025:1029	arg1	elongation					1011:1020	∼6 % elongation	1006:1020	∼6 % elongation	1006:1020	Consequently, NFC/(PEI/SiO2)-AF paper-based composites showed better tensile toughness (∼6 % elongation at break) and mechanical strength (∼36.28 MPa), in comparison with NFC/AF.
32718657	1	23	theme	electrical	249:258	arg1	insulation					260:269	electrical insulation	249:269	electrical insulation	249:269	Flexible paper-based nanocomposites dielectrics are of crucial importance in electrical insulation and advanced electrical power systems.
32718657	3	24	theme	dielectric	469:478	arg1	property					480:487	the dielectric property	465:487	the dielectric property of the composites	465:505	In order to improve the dielectric property of the composites, carboxylated nano-SiO2 was chemically coated onto aramid fibrid via molecular self-assembly with the aid of phosphoric acid (PA) pretreatment and subsequent polyethyleneimine (PEI) functionalization.
32718657	3	25	with	self-assembly	586:598	arg1	the					605:607	the	605:607	the	605:607	In order to improve the dielectric property of the composites, carboxylated nano-SiO2 was chemically coated onto aramid fibrid via molecular self-assembly with the aid of phosphoric acid (PA) pretreatment and subsequent polyethyleneimine (PEI) functionalization.
32718657	3	26	theme	subsequent	654:663	arg1	PEI					684:686	PEI	684:686	PEI	684:686	In order to improve the dielectric property of the composites, carboxylated nano-SiO2 was chemically coated onto aramid fibrid via molecular self-assembly with the aid of phosphoric acid (PA) pretreatment and subsequent polyethyleneimine (PEI) functionalization.
32718657	3	26	theme	subsequent	654:663	arg1	polyethyleneimine					665:681	subsequent polyethyleneimine	654:681	subsequent polyethyleneimine (PEI)	654:687	In order to improve the dielectric property of the composites, carboxylated nano-SiO2 was chemically coated onto aramid fibrid via molecular self-assembly with the aid of phosphoric acid (PA) pretreatment and subsequent polyethyleneimine (PEI) functionalization.
32718657	5	27	dep	toughness	995:1003	arg1	elongation					1011:1020	∼6 % elongation	1006:1020	∼6 % elongation	1006:1020	Consequently, NFC/(PEI/SiO2)-AF paper-based composites showed better tensile toughness (∼6 % elongation at break) and mechanical strength (∼36.28 MPa), in comparison with NFC/AF.
32718657	3	28	theme	carboxylated	508:519	arg1	nano-SiO2					521:529	carboxylated nano-SiO2	508:529	carboxylated nano-SiO2	508:529	In order to improve the dielectric property of the composites, carboxylated nano-SiO2 was chemically coated onto aramid fibrid via molecular self-assembly with the aid of phosphoric acid (PA) pretreatment and subsequent polyethyleneimine (PEI) functionalization.
32718657	0	29	theme	cellulose/aramid	40:55	arg1	composites					76:85	high-performance nanofibrillated cellulose/aramid fibrid paper-based composites	7:85	high-performance nanofibrillated cellulose/aramid fibrid paper-based composites via polyethyleneimine-assisted decoration of silica nanoparticle onto aramid fibrid	7:169	Toward high-performance nanofibrillated cellulose/aramid fibrid paper-based composites via polyethyleneimine-assisted decoration of silica nanoparticle onto aramid fibrid.
32718657	5	30	theme	tensile	987:993	arg1	toughness					995:1003	better tensile toughness	980:1003	better tensile toughness (∼6 % elongation at break)	980:1030	Consequently, NFC/(PEI/SiO2)-AF paper-based composites showed better tensile toughness (∼6 % elongation at break) and mechanical strength (∼36.28 MPa), in comparison with NFC/AF.
32718657	0	31	theme	nanofibrillated	24:38	arg1	composites					76:85	high-performance nanofibrillated cellulose/aramid fibrid paper-based composites	7:85	high-performance nanofibrillated cellulose/aramid fibrid paper-based composites via polyethyleneimine-assisted decoration of silica nanoparticle onto aramid fibrid	7:169	Toward high-performance nanofibrillated cellulose/aramid fibrid paper-based composites via polyethyleneimine-assisted decoration of silica nanoparticle onto aramid fibrid.
32718657	3	32	theme	phosphoric	616:625	arg1	PA					633:634	PA	633:634	PA	633:634	In order to improve the dielectric property of the composites, carboxylated nano-SiO2 was chemically coated onto aramid fibrid via molecular self-assembly with the aid of phosphoric acid (PA) pretreatment and subsequent polyethyleneimine (PEI) functionalization.
32718657	3	32	theme	phosphoric	616:625	arg1	acid					627:630	phosphoric acid	616:630	phosphoric acid (PA) pretreatment	616:648	In order to improve the dielectric property of the composites, carboxylated nano-SiO2 was chemically coated onto aramid fibrid via molecular self-assembly with the aid of phosphoric acid (PA) pretreatment and subsequent polyethyleneimine (PEI) functionalization.
32718657	2	33	theme	NFC/AF	373:378	arg1	composite					381:389	a novel nanofibrillated cellulose/aramid fibrid (NFC/AF) composite	324:389	a novel nanofibrillated cellulose/aramid fibrid (NFC/AF) composite	324:389	In this work, a novel nanofibrillated cellulose/aramid fibrid (NFC/AF) composite was fabricated by vacuum-assisted filtration process.
32718657	1	34	theme	advanced	275:282	arg1	systems					301:307	advanced electrical power systems	275:307	advanced electrical power systems	275:307	Flexible paper-based nanocomposites dielectrics are of crucial importance in electrical insulation and advanced electrical power systems.
32718657	0	35	theme	paper-based	64:74	arg1	composites					76:85	high-performance nanofibrillated cellulose/aramid fibrid paper-based composites	7:85	high-performance nanofibrillated cellulose/aramid fibrid paper-based composites via polyethyleneimine-assisted decoration of silica nanoparticle onto aramid fibrid	7:169	Toward high-performance nanofibrillated cellulose/aramid fibrid paper-based composites via polyethyleneimine-assisted decoration of silica nanoparticle onto aramid fibrid.
32718657	2	36	theme	vacuum-assisted	409:423	arg1	process					436:442	vacuum-assisted filtration process	409:442	vacuum-assisted filtration process	409:442	In this work, a novel nanofibrillated cellulose/aramid fibrid (NFC/AF) composite was fabricated by vacuum-assisted filtration process.
32718657	7	37	theme	high-performance	1285:1300	arg1	composites					1313:1322	high-performance dielectric composites	1285:1322	high-performance dielectric composites	1285:1322	Accordingly, this work provides a facile approach to fabricate high-performance dielectric composites especially for high-temperature electrical insulation applications.
32718657	5	38	theme	mechanical	1036:1045	arg1	strength					1047:1054	mechanical strength	1036:1054	mechanical strength (∼36.28 MPa)	1036:1067	Consequently, NFC/(PEI/SiO2)-AF paper-based composites showed better tensile toughness (∼6 % elongation at break) and mechanical strength (∼36.28 MPa), in comparison with NFC/AF.
32718657	5	38	theme	mechanical	1036:1045	arg1	∼36.28 MPa					1057:1066	∼36.28 MPa	1057:1066	∼36.28 MPa	1057:1066	Consequently, NFC/(PEI/SiO2)-AF paper-based composites showed better tensile toughness (∼6 % elongation at break) and mechanical strength (∼36.28 MPa), in comparison with NFC/AF.
32718657	7	39	theme	insulation	1367:1376	arg1	applications					1378:1389	high-temperature electrical insulation applications	1339:1389	high-temperature electrical insulation applications	1339:1389	Accordingly, this work provides a facile approach to fabricate high-performance dielectric composites especially for high-temperature electrical insulation applications.
32718657	1	40	theme	electrical	284:293	arg1	systems					301:307	advanced electrical power systems	275:307	advanced electrical power systems	275:307	Flexible paper-based nanocomposites dielectrics are of crucial importance in electrical insulation and advanced electrical power systems.
32718657	0	41	theme	fibrid	57:62	arg1	composites					76:85	high-performance nanofibrillated cellulose/aramid fibrid paper-based composites	7:85	high-performance nanofibrillated cellulose/aramid fibrid paper-based composites via polyethyleneimine-assisted decoration of silica nanoparticle onto aramid fibrid	7:169	Toward high-performance nanofibrillated cellulose/aramid fibrid paper-based composites via polyethyleneimine-assisted decoration of silica nanoparticle onto aramid fibrid.
32718657	3	42	theme	composites	496:505	arg1	property					480:487	the dielectric property	465:487	the dielectric property of the composites	465:505	In order to improve the dielectric property of the composites, carboxylated nano-SiO2 was chemically coated onto aramid fibrid via molecular self-assembly with the aid of phosphoric acid (PA) pretreatment and subsequent polyethyleneimine (PEI) functionalization.
32718657	1	43	theme	power	295:299	arg1	systems					301:307	advanced electrical power systems	275:307	advanced electrical power systems	275:307	Flexible paper-based nanocomposites dielectrics are of crucial importance in electrical insulation and advanced electrical power systems.
32718657	4	44	theme	enhanced	865:872	arg1	interaction					886:896	enhanced interfacial interaction	865:896	enhanced interfacial interaction between AF and NFC	865:915	It was found that composites prepared by NFC and (PEI/SiO2)-modified AF (after crosslinking) ((PEI/SiO2)-AF) showed dense structure, which was mainly due to enhanced interfacial interaction between AF and NFC.
32718657	2	45	theme	filtration	425:434	arg1	process					436:442	vacuum-assisted filtration process	409:442	vacuum-assisted filtration process	409:442	In this work, a novel nanofibrillated cellulose/aramid fibrid (NFC/AF) composite was fabricated by vacuum-assisted filtration process.
32718657	3	46	dep	the	605:607	arg1	aid					609:611	aid	609:611	aid	609:611	In order to improve the dielectric property of the composites, carboxylated nano-SiO2 was chemically coated onto aramid fibrid via molecular self-assembly with the aid of phosphoric acid (PA) pretreatment and subsequent polyethyleneimine (PEI) functionalization.
32718657	1	47	theme	Flexible	172:179	arg1	dielectrics					208:218	Flexible paper-based nanocomposites dielectrics	172:218	Flexible paper-based nanocomposites dielectrics	172:218	Flexible paper-based nanocomposites dielectrics are of crucial importance in electrical insulation and advanced electrical power systems.
32718657	7	48	theme	dielectric	1302:1311	arg1	composites					1313:1322	high-performance dielectric composites	1285:1322	high-performance dielectric composites	1285:1322	Accordingly, this work provides a facile approach to fabricate high-performance dielectric composites especially for high-temperature electrical insulation applications.
32718657	2	49	theme	novel	326:330	arg1	composite					381:389	a novel nanofibrillated cellulose/aramid fibrid (NFC/AF) composite	324:389	a novel nanofibrillated cellulose/aramid fibrid (NFC/AF) composite	324:389	In this work, a novel nanofibrillated cellulose/aramid fibrid (NFC/AF) composite was fabricated by vacuum-assisted filtration process.
32718657	4	50	theme	PEI/SiO2	758:765	arg1	AF					777:778	(PEI/SiO2)-modified AF	757:778	(PEI/SiO2)-modified AF (after crosslinking) ((PEI/SiO2)-AF)	757:815	It was found that composites prepared by NFC and (PEI/SiO2)-modified AF (after crosslinking) ((PEI/SiO2)-AF) showed dense structure, which was mainly due to enhanced interfacial interaction between AF and NFC.
32718657	4	50	theme	PEI/SiO2	758:765	arg1	-AF					812:814	(PEI/SiO2)-AF	802:814	(PEI/SiO2)-AF	802:814	It was found that composites prepared by NFC and (PEI/SiO2)-modified AF (after crosslinking) ((PEI/SiO2)-AF) showed dense structure, which was mainly due to enhanced interfacial interaction between AF and NFC.
32718657	1	51	theme	paper-based	181:191	arg1	dielectrics					208:218	Flexible paper-based nanocomposites dielectrics	172:218	Flexible paper-based nanocomposites dielectrics	172:218	Flexible paper-based nanocomposites dielectrics are of crucial importance in electrical insulation and advanced electrical power systems.
32718657	3	52	theme	acid	627:630	arg1	pretreatment					637:648	phosphoric acid (PA) pretreatment	616:648	phosphoric acid (PA) pretreatment	616:648	In order to improve the dielectric property of the composites, carboxylated nano-SiO2 was chemically coated onto aramid fibrid via molecular self-assembly with the aid of phosphoric acid (PA) pretreatment and subsequent polyethyleneimine (PEI) functionalization.
32718657	4	53	theme	dense	824:828	arg1	structure					830:838	dense structure	824:838	dense structure	824:838	It was found that composites prepared by NFC and (PEI/SiO2)-modified AF (after crosslinking) ((PEI/SiO2)-AF) showed dense structure, which was mainly due to enhanced interfacial interaction between AF and NFC.
32718657	1	54	theme	nanocomposites	193:206	arg1	dielectrics					208:218	Flexible paper-based nanocomposites dielectrics	172:218	Flexible paper-based nanocomposites dielectrics	172:218	Flexible paper-based nanocomposites dielectrics are of crucial importance in electrical insulation and advanced electrical power systems.
32718657	6	55	theme	thermal	1157:1163	arg1	stability					1165:1173	thermal stability	1157:1173	thermal stability	1157:1173	More importantly, the electrical insulation performance and thermal stability of the composites were significantly improved.
32718657	5	56	theme	paper-based	950:960	arg1	composites					962:971	NFC/(PEI/SiO2)-AF paper-based composites	932:971	NFC/(PEI/SiO2)-AF paper-based composites	932:971	Consequently, NFC/(PEI/SiO2)-AF paper-based composites showed better tensile toughness (∼6 % elongation at break) and mechanical strength (∼36.28 MPa), in comparison with NFC/AF.
32718657	7	57	theme	facile	1256:1261	arg1	approach					1263:1270	a facile approach	1254:1270	a facile approach to fabricate high-performance dielectric composites especially for high-temperature electrical insulation applications	1254:1389	Accordingly, this work provides a facile approach to fabricate high-performance dielectric composites especially for high-temperature electrical insulation applications.
32718657	5	58	theme	%	1009:1009	arg1	elongation					1011:1020	∼6 % elongation	1006:1020	∼6 % elongation	1006:1020	Consequently, NFC/(PEI/SiO2)-AF paper-based composites showed better tensile toughness (∼6 % elongation at break) and mechanical strength (∼36.28 MPa), in comparison with NFC/AF.
32718657	6	59	theme	electrical	1119:1128	arg1	performance					1141:1151	the electrical insulation performance	1115:1151	the electrical insulation performance	1115:1151	More importantly, the electrical insulation performance and thermal stability of the composites were significantly improved.
32718657	0	60	theme	polyethyleneimine-assisted	91:116	arg1	decoration					118:127	polyethyleneimine-assisted decoration	91:127	polyethyleneimine-assisted decoration of silica nanoparticle onto aramid fibrid	91:169	Toward high-performance nanofibrillated cellulose/aramid fibrid paper-based composites via polyethyleneimine-assisted decoration of silica nanoparticle onto aramid fibrid.
32718657	7	61	theme	high-temperature	1339:1354	arg1	applications					1378:1389	high-temperature electrical insulation applications	1339:1389	high-temperature electrical insulation applications	1339:1389	Accordingly, this work provides a facile approach to fabricate high-performance dielectric composites especially for high-temperature electrical insulation applications.
32718657	1	62	from	importance	235:244	arg1	systems					301:307	advanced electrical power systems	275:307	advanced electrical power systems	275:307	Flexible paper-based nanocomposites dielectrics are of crucial importance in electrical insulation and advanced electrical power systems.
32718657	1	62	from	importance	235:244	arg1	insulation					260:269	electrical insulation	249:269	electrical insulation	249:269	Flexible paper-based nanocomposites dielectrics are of crucial importance in electrical insulation and advanced electrical power systems.
32718657	3	63	dep	pretreatment	637:648	arg1	functionalization					689:705	functionalization	689:705	functionalization	689:705	In order to improve the dielectric property of the composites, carboxylated nano-SiO2 was chemically coated onto aramid fibrid via molecular self-assembly with the aid of phosphoric acid (PA) pretreatment and subsequent polyethyleneimine (PEI) functionalization.
32118530	1	0	theme	spread	340:345	arg1	tracking					324:331	accurate tracking	315:331	accurate tracking of the spread of specific lineages	315:366	Multiply antibiotic-resistant Acinetobacter baumannii infections are a global public health concern and accurate tracking of the spread of specific lineages is needed.
32118530	9	1	theme	OCL	1911:1913	arg1	type					1915:1918	a KL or OCL type	1903:1918	type	1915:1918	The failure to assign a KL or OCL type may indicate incomplete or poor-quality genomes.
32118530	0	2	theme	reference	166:174	arg1	databases					176:184	curated reference databases	158:184	curated reference databases compatible with Kaptive	158:208	Identification of Acinetobacter baumannii loci for capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive.
32118530	1	3	theme	global	282:287	arg1	infections					265:274	Multiply antibiotic-resistant Acinetobacter baumannii infections	211:274	Multiply antibiotic-resistant Acinetobacter baumannii infections	211:274	Multiply antibiotic-resistant Acinetobacter baumannii infections are a global public health concern and accurate tracking of the spread of specific lineages is needed.
32118530	1	3	theme	global	282:287	arg1	concern					303:309	a global public health concern	280:309	a global public health concern	280:309	Multiply antibiotic-resistant Acinetobacter baumannii infections are a global public health concern and accurate tracking of the spread of specific lineages is needed.
32118530	0	4	theme	compatible	186:195	arg1	databases					176:184	curated reference databases	158:184	curated reference databases compatible with Kaptive	158:208	Identification of Acinetobacter baumannii loci for capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive.
32118530	3	5	theme	outer	569:573	arg1	OC					581:582	OC	581:582	OC	581:582	The outer core (OC) of lipooligosaccharide also exhibits variation.
32118530	3	5	theme	outer	569:573	arg1	core					575:578	The outer core	565:578	The outer core (OC) of lipooligosaccharide	565:606	The outer core (OC) of lipooligosaccharide also exhibits variation.
32118530	1	6	theme	health	296:301	arg1	infections					265:274	Multiply antibiotic-resistant Acinetobacter baumannii infections	211:274	Multiply antibiotic-resistant Acinetobacter baumannii infections	211:274	Multiply antibiotic-resistant Acinetobacter baumannii infections are a global public health concern and accurate tracking of the spread of specific lineages is needed.
32118530	1	6	theme	health	296:301	arg1	concern					303:309	a global public health concern	280:309	a global public health concern	280:309	Multiply antibiotic-resistant Acinetobacter baumannii infections are a global public health concern and accurate tracking of the spread of specific lineages is needed.
32118530	4	7	theme	information	674:684	arg1	advantage					648:656	better advantage	641:656	better advantage of the untapped information available in whole genome sequences	641:720	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	8	8	theme	common	1723:1728	arg1	KL					1730:1731	common KL	1723:1731	common KL	1723:1731	Among the genomes retrieved from NCBI, Kaptive detected known KL and OCL in 87 and 90 % of genomes, respectively, indicating that the majority of common KL and OCL types are captured within the databases; 13 of the 92 KL in the database were not detected in any publicly available whole genome assembly.
32118530	6	9	theme	genome	1424:1429	arg1	assemblies					1431:1440	3386 A. baumannii genome assemblies	1406:1440	(b) 3386 A. baumannii genome assemblies downloaded from NCBI	1402:1461	These databases are compatible with Kaptive, a tool for in silico typing of bacterial surface polysaccharide loci, and their utility was validated using (a) >630 assembled A. baumannii draft genomes for which the KL and OCL regions had been previously typed manually, and (b) 3386 A. baumannii genome assemblies downloaded from NCBI.
32118530	11	10	theme	sequence	2064:2071	arg1	typing					2073:2078	multilocus sequence typing	2053:2078	multilocus sequence typing (Institut Pasteur scheme)	2053:2104	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	11	10	theme	sequence	2064:2071	arg1	scheme					2098:2103	Institut Pasteur scheme	2081:2103	Institut Pasteur scheme	2081:2103	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	6	11	theme	A.	1411:1412	arg1	assemblies					1431:1440	3386 A. baumannii genome assemblies	1406:1440	(b) 3386 A. baumannii genome assemblies downloaded from NCBI	1402:1461	These databases are compatible with Kaptive, a tool for in silico typing of bacterial surface polysaccharide loci, and their utility was validated using (a) >630 assembled A. baumannii draft genomes for which the KL and OCL regions had been previously typed manually, and (b) 3386 A. baumannii genome assemblies downloaded from NCBI.
32118530	6	12	theme	surface	1216:1222	arg1	loci					1239:1242	bacterial surface polysaccharide loci	1206:1242	bacterial surface polysaccharide loci	1206:1242	These databases are compatible with Kaptive, a tool for in silico typing of bacterial surface polysaccharide loci, and their utility was validated using (a) >630 assembled A. baumannii draft genomes for which the KL and OCL regions had been previously typed manually, and (b) 3386 A. baumannii genome assemblies downloaded from NCBI.
32118530	6	13	dep	assemblies	1431:1440	arg1	b					1403:1403	b	1403:1403	b	1403:1403	These databases are compatible with Kaptive, a tool for in silico typing of bacterial surface polysaccharide loci, and their utility was validated using (a) >630 assembled A. baumannii draft genomes for which the KL and OCL regions had been previously typed manually, and (b) 3386 A. baumannii genome assemblies downloaded from NCBI.
32118530	0	14	theme	OCL	116:118	arg1	synthesis					121:129	capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis	51:129	capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive	51:208	Identification of Acinetobacter baumannii loci for capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive.
32118530	5	15	theme	unique	1023:1028	arg1	KL					1030:1031	a unique KL	1021:1031	a unique KL	1021:1031	Each entry has been assigned a unique KL or OCL number, and is fully annotated using a simple, transparent and standardized nomenclature.
32118530	11	16	theme	Pasteur	2090:2096	arg1	typing					2073:2078	multilocus sequence typing	2053:2078	multilocus sequence typing (Institut Pasteur scheme)	2053:2104	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	11	16	theme	Pasteur	2090:2096	arg1	scheme					2098:2103	Institut Pasteur scheme	2081:2103	Institut Pasteur scheme	2081:2103	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	4	17	from	database	909:916	arg1	locus					814:818	the locus	810:818	the locus encoding proteins responsible for biosynthesis and export of CPS (K locus)	810:893	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	8	18	theme	known	1633:1637	arg1	KL					1639:1640	known KL	1633:1640	known KL	1633:1640	Among the genomes retrieved from NCBI, Kaptive detected known KL and OCL in 87 and 90 % of genomes, respectively, indicating that the majority of common KL and OCL types are captured within the databases; 13 of the 92 KL in the database were not detected in any publicly available whole genome assembly.
32118530	1	19	theme	antibiotic-resistant	220:239	arg1	infections					265:274	Multiply antibiotic-resistant Acinetobacter baumannii infections	211:274	Multiply antibiotic-resistant Acinetobacter baumannii infections	211:274	Multiply antibiotic-resistant Acinetobacter baumannii infections are a global public health concern and accurate tracking of the spread of specific lineages is needed.
32118530	1	19	theme	antibiotic-resistant	220:239	arg1	concern					303:309	a global public health concern	280:309	a global public health concern	280:309	Multiply antibiotic-resistant Acinetobacter baumannii infections are a global public health concern and accurate tracking of the spread of specific lineages is needed.
32118530	0	20	theme	genome	134:139	arg1	assemblies					141:150	genome assemblies	134:150	genome assemblies using curated reference databases compatible with Kaptive	134:208	Identification of Acinetobacter baumannii loci for capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive.
32118530	3	21	theme	lipooligosaccharide	588:606	arg1	OC					581:582	OC	581:582	OC	581:582	The outer core (OC) of lipooligosaccharide also exhibits variation.
32118530	3	21	theme	lipooligosaccharide	588:606	arg1	core					575:578	The outer core	565:578	The outer core (OC) of lipooligosaccharide	565:606	The outer core (OC) of lipooligosaccharide also exhibits variation.
32118530	11	22	theme	clones	2312:2317	arg1	collections					2291:2301	collections	2291:2301	collections of other clones comprising >20 isolates each (ST10, ST25, and ST140)	2291:2370	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	8	23	theme	types	1741:1745	arg1	majority					1711:1718	the majority	1707:1718	the majority of common KL and OCL types	1707:1745	Among the genomes retrieved from NCBI, Kaptive detected known KL and OCL in 87 and 90 % of genomes, respectively, indicating that the majority of common KL and OCL types are captured within the databases; 13 of the 92 KL in the database were not detected in any publicly available whole genome assembly.
32118530	6	24	theme	loci	1239:1242	arg1	typing					1196:1201	in silico typing	1186:1201	in silico typing of bacterial surface polysaccharide loci	1186:1242	These databases are compatible with Kaptive, a tool for in silico typing of bacterial surface polysaccharide loci, and their utility was validated using (a) >630 assembled A. baumannii draft genomes for which the KL and OCL regions had been previously typed manually, and (b) 3386 A. baumannii genome assemblies downloaded from NCBI.
32118530	4	25	from	locus	814:818	arg1	database					759:766	a curated reference database	739:766	a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus)	739:893	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	4	25	from	locus	814:818	arg1	database					909:916	a second database	900:916	a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus)	900:989	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	4	25	from	locus	814:818	arg1	clusters					798:805	92 publicly available gene clusters	771:805	92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus)	771:893	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	11	26	theme	distributed	2237:2247	arg1	ST1					2257:2259	ST1	2257:2259	ST1 of GC1 and ST2 of GC2	2257:2281	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	11	26	theme	distributed	2237:2247	arg1	clones					2249:2254	the two predominant globally distributed clones	2208:2254	the two predominant globally distributed clones	2208:2254	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	4	27	theme	genome	705:710	arg1	sequences					712:720	whole genome sequences	699:720	whole genome sequences	699:720	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	1	28	theme	specific	350:357	arg1	lineages					359:366	specific lineages	350:366	specific lineages	350:366	Multiply antibiotic-resistant Acinetobacter baumannii infections are a global public health concern and accurate tracking of the spread of specific lineages is needed.
32118530	2	29	theme	susceptibility	502:515	arg1	polysaccharide					434:447	capsular polysaccharide	425:447	capsular polysaccharide (CPS)	425:453	Variation in the composition and structure of capsular polysaccharide (CPS), a critical determinant of virulence and phage susceptibility, makes it an attractive epidemiological marker.
32118530	2	29	theme	susceptibility	502:515	arg1	determinant					467:477	a critical determinant	456:477	a critical determinant of virulence and phage susceptibility	456:515	Variation in the composition and structure of capsular polysaccharide (CPS), a critical determinant of virulence and phage susceptibility, makes it an attractive epidemiological marker.
32118530	10	30	theme	further	1978:1984	arg1	variants					1992:1999	further novel variants	1978:1999	further novel variants	1978:1999	However, further novel variants may remain to be documented.
32118530	2	31	theme	epidemiological	541:555	arg1	marker					557:562	an attractive epidemiological marker	527:562	an attractive epidemiological marker	527:562	Variation in the composition and structure of capsular polysaccharide (CPS), a critical determinant of virulence and phage susceptibility, makes it an attractive epidemiological marker.
32118530	11	32	theme	sequence	2168:2175	arg1	type					2177:2180	a single sequence type	2159:2180	a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2	2159:2281	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	0	33	theme	lipooligosaccharide	84:102	arg1	synthesis					121:129	capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis	51:129	capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive	51:208	Identification of Acinetobacter baumannii loci for capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive.
32118530	8	34	theme	OCL	1737:1739	arg1	types					1741:1745	OCL types	1737:1745	OCL types	1737:1745	Among the genomes retrieved from NCBI, Kaptive detected known KL and OCL in 87 and 90 % of genomes, respectively, indicating that the majority of common KL and OCL types are captured within the databases; 13 of the 92 KL in the database were not detected in any publicly available whole genome assembly.
32118530	8	35	theme	genomes	1668:1674	arg1	genomes					1668:1674	genomes	1668:1674	genomes	1668:1674	Among the genomes retrieved from NCBI, Kaptive detected known KL and OCL in 87 and 90 % of genomes, respectively, indicating that the majority of common KL and OCL types are captured within the databases; 13 of the 92 KL in the database were not detected in any publicly available whole genome assembly.
32118530	8	35	theme	genomes	1668:1674	arg1	%					1663:1663	87 and 90 %	1653:1663	87 and 90 % of genomes	1653:1674	Among the genomes retrieved from NCBI, Kaptive detected known KL and OCL in 87 and 90 % of genomes, respectively, indicating that the majority of common KL and OCL types are captured within the databases; 13 of the 92 KL in the database were not detected in any publicly available whole genome assembly.
32118530	6	36	with	compatible	1150:1159	arg1	Kaptive					1166:1172	Kaptive	1166:1172	Kaptive	1166:1172	These databases are compatible with Kaptive, a tool for in silico typing of bacterial surface polysaccharide loci, and their utility was validated using (a) >630 assembled A. baumannii draft genomes for which the KL and OCL regions had been previously typed manually, and (b) 3386 A. baumannii genome assemblies downloaded from NCBI.
32118530	6	36	with	compatible	1150:1159	arg1	tool					1177:1180	a tool	1175:1180	a tool for in silico typing of bacterial surface polysaccharide loci	1175:1242	These databases are compatible with Kaptive, a tool for in silico typing of bacterial surface polysaccharide loci, and their utility was validated using (a) >630 assembled A. baumannii draft genomes for which the KL and OCL regions had been previously typed manually, and (b) 3386 A. baumannii genome assemblies downloaded from NCBI.
32118530	4	37	theme	gene	793:796	arg1	clusters					798:805	92 publicly available gene clusters	771:805	92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus)	771:893	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	0	38	with	compatible	186:195	arg1	Kaptive					202:208	Kaptive	202:208	Kaptive	202:208	Identification of Acinetobacter baumannii loci for capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive.
32118530	0	39	theme	core	110:113	arg1	synthesis					121:129	capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis	51:129	capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive	51:208	Identification of Acinetobacter baumannii loci for capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive.
32118530	7	40	theme	100 	1561:1564	arg1	%					1565:1565	%	1565:1565	%	1565:1565	Among the previously typed genomes, Kaptive was able to confidently assign KL and OCL types with 100 % accuracy.
32118530	2	41	theme	critical	458:465	arg1	polysaccharide					434:447	capsular polysaccharide	425:447	capsular polysaccharide (CPS)	425:453	Variation in the composition and structure of capsular polysaccharide (CPS), a critical determinant of virulence and phage susceptibility, makes it an attractive epidemiological marker.
32118530	2	41	theme	critical	458:465	arg1	determinant					467:477	a critical determinant	456:477	a critical determinant of virulence and phage susceptibility	456:515	Variation in the composition and structure of capsular polysaccharide (CPS), a critical determinant of virulence and phage susceptibility, makes it an attractive epidemiological marker.
32118530	4	42	theme	reference	749:757	arg1	database					759:766	a curated reference database	739:766	a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus)	739:893	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	6	43	theme	OCL	1350:1352	arg1	regions					1354:1360	the KL and OCL regions	1339:1360	regions	1354:1360	These databases are compatible with Kaptive, a tool for in silico typing of bacterial surface polysaccharide loci, and their utility was validated using (a) >630 assembled A. baumannii draft genomes for which the KL and OCL regions had been previously typed manually, and (b) 3386 A. baumannii genome assemblies downloaded from NCBI.
32118530	5	44	theme	standardized	1103:1114	arg1	nomenclature					1116:1127	a simple, transparent and standardized nomenclature	1077:1127	a simple, transparent and standardized nomenclature	1077:1127	Each entry has been assigned a unique KL or OCL number, and is fully annotated using a simple, transparent and standardized nomenclature.
32118530	6	45	theme	KL	1343:1344	arg1	regions					1354:1360	the KL and OCL regions	1339:1360	regions	1354:1360	These databases are compatible with Kaptive, a tool for in silico typing of bacterial surface polysaccharide loci, and their utility was validated using (a) >630 assembled A. baumannii draft genomes for which the KL and OCL regions had been previously typed manually, and (b) 3386 A. baumannii genome assemblies downloaded from NCBI.
32118530	1	46	theme	accurate	315:322	arg1	tracking					324:331	accurate tracking	315:331	accurate tracking of the spread of specific lineages	315:366	Multiply antibiotic-resistant Acinetobacter baumannii infections are a global public health concern and accurate tracking of the spread of specific lineages is needed.
32118530	2	47	theme	polysaccharide	434:447	arg1	structure					412:420	structure	412:420	structure	412:420	Variation in the composition and structure of capsular polysaccharide (CPS), a critical determinant of virulence and phage susceptibility, makes it an attractive epidemiological marker.
32118530	2	47	theme	polysaccharide	434:447	arg1	composition					396:406	composition	396:406	composition	396:406	Variation in the composition and structure of capsular polysaccharide (CPS), a critical determinant of virulence and phage susceptibility, makes it an attractive epidemiological marker.
32118530	0	48	theme	loci	42:45	arg1	Identification					0:13	Identification	0:13	Identification of Acinetobacter baumannii loci for capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive.	0:209	Identification of Acinetobacter baumannii loci for capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive.
32118530	5	49	theme	simple	1079:1084	arg1	nomenclature					1116:1127	a simple, transparent and standardized nomenclature	1077:1127	a simple, transparent and standardized nomenclature	1077:1127	Each entry has been assigned a unique KL or OCL number, and is fully annotated using a simple, transparent and standardized nomenclature.
32118530	11	50	dep	isolates	2334:2341	arg1	ST140					2365:2369	ST140	2365:2369	ST140	2365:2369	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	11	50	dep	isolates	2334:2341	arg1	ST10					2349:2352	ST10	2349:2352	ST10	2349:2352	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	11	50	dep	isolates	2334:2341	arg1	each					2343:2346	each	2343:2346	each	2343:2346	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	11	50	dep	isolates	2334:2341	arg1	isolates					2334:2341	>20 isolates	2330:2341	>20 isolates each (ST10, ST25, and ST140)	2330:2370	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	11	50	dep	isolates	2334:2341	arg1	ST25					2355:2358	ST25	2355:2358	ST25	2355:2358	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	12	51	theme	locus	2531:2535	arg1	types					2537:2541	further locus types	2523:2541	further locus types	2523:2541	The databases are available at https://github.com/katholt/Kaptive and will be updated as further locus types become available.
32118530	11	52	theme	within-clone	2394:2405	arg1	replacement					2407:2417	extensive within-clone replacement	2384:2417	extensive within-clone replacement of these loci	2384:2431	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	0	53	theme	capsular	51:58	arg1	KL					76:77	KL	76:77	KL	76:77	Identification of Acinetobacter baumannii loci for capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive.
32118530	0	53	theme	capsular	51:58	arg1	polysaccharide					60:73	capsular polysaccharide	51:73	capsular polysaccharide (KL)	51:78	Identification of Acinetobacter baumannii loci for capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive.
32118530	5	54	theme	transparent	1087:1097	arg1	nomenclature					1116:1127	a simple, transparent and standardized nomenclature	1077:1127	a simple, transparent and standardized nomenclature	1077:1127	Each entry has been assigned a unique KL or OCL number, and is fully annotated using a simple, transparent and standardized nomenclature.
32118530	0	55	from	synthesis	121:129	arg1	assemblies					141:150	genome assemblies	134:150	genome assemblies using curated reference databases compatible with Kaptive	134:208	Identification of Acinetobacter baumannii loci for capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive.
32118530	9	56	theme	KL	1905:1906	arg1	type					1915:1918	a KL or OCL type	1903:1918	type	1915:1918	The failure to assign a KL or OCL type may indicate incomplete or poor-quality genomes.
32118530	8	57	theme	genome	1864:1869	arg1	assembly					1871:1878	any publicly available whole genome assembly	1835:1878	any publicly available whole genome assembly	1835:1878	Among the genomes retrieved from NCBI, Kaptive detected known KL and OCL in 87 and 90 % of genomes, respectively, indicating that the majority of common KL and OCL types are captured within the databases; 13 of the 92 KL in the database were not detected in any publicly available whole genome assembly.
32118530	4	58	theme	core	962:965	arg1	biosynthesis					967:978	outer core biosynthesis	956:978	outer core biosynthesis (OC locus)	956:989	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	4	58	theme	core	962:965	arg1	locus					984:988	OC locus	981:988	OC locus	981:988	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	6	59	theme	polysaccharide	1224:1237	arg1	loci					1239:1242	bacterial surface polysaccharide loci	1206:1242	bacterial surface polysaccharide loci	1206:1242	These databases are compatible with Kaptive, a tool for in silico typing of bacterial surface polysaccharide loci, and their utility was validated using (a) >630 assembled A. baumannii draft genomes for which the KL and OCL regions had been previously typed manually, and (b) 3386 A. baumannii genome assemblies downloaded from NCBI.
32118530	9	60	theme	poor-quality	1947:1958	arg1	genomes					1960:1966	incomplete or poor-quality genomes	1933:1966	incomplete or poor-quality genomes	1933:1966	The failure to assign a KL or OCL type may indicate incomplete or poor-quality genomes.
32118530	4	61	from	sequences	712:720	arg1	available					686:694	available	686:694	available	686:694	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	4	62	theme	CPS	881:883	arg1	export					871:876	export	871:876	export	871:876	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	4	62	theme	CPS	881:883	arg1	biosynthesis					854:865	biosynthesis	854:865	biosynthesis	854:865	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	11	63	theme	OCL	2131:2133	arg1	types					2135:2139	multiple KL and OCL types	2115:2139	types	2135:2139	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	6	64	theme	bacterial	1206:1214	arg1	loci					1239:1242	bacterial surface polysaccharide loci	1206:1242	bacterial surface polysaccharide loci	1206:1242	These databases are compatible with Kaptive, a tool for in silico typing of bacterial surface polysaccharide loci, and their utility was validated using (a) >630 assembled A. baumannii draft genomes for which the KL and OCL regions had been previously typed manually, and (b) 3386 A. baumannii genome assemblies downloaded from NCBI.
32118530	11	65	theme	predominant	2216:2226	arg1	ST1					2257:2259	ST1	2257:2259	ST1 of GC1 and ST2 of GC2	2257:2281	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	11	65	theme	predominant	2216:2226	arg1	clones					2249:2254	the two predominant globally distributed clones	2208:2254	the two predominant globally distributed clones	2208:2254	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	1	66	theme	public	289:294	arg1	infections					265:274	Multiply antibiotic-resistant Acinetobacter baumannii infections	211:274	Multiply antibiotic-resistant Acinetobacter baumannii infections	211:274	Multiply antibiotic-resistant Acinetobacter baumannii infections are a global public health concern and accurate tracking of the spread of specific lineages is needed.
32118530	1	66	theme	public	289:294	arg1	concern					303:309	a global public health concern	280:309	a global public health concern	280:309	Multiply antibiotic-resistant Acinetobacter baumannii infections are a global public health concern and accurate tracking of the spread of specific lineages is needed.
32118530	4	67	theme	K	886:886	arg1	CPS					881:883	CPS	881:883	CPS (K locus)	881:893	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	4	67	theme	K	886:886	arg1	locus					888:892	K locus	886:892	K locus	886:892	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	6	68	dep	A.	1411:1412	arg1	baumannii					1414:1422	baumannii	1414:1422	baumannii	1414:1422	These databases are compatible with Kaptive, a tool for in silico typing of bacterial surface polysaccharide loci, and their utility was validated using (a) >630 assembled A. baumannii draft genomes for which the KL and OCL regions had been previously typed manually, and (b) 3386 A. baumannii genome assemblies downloaded from NCBI.
32118530	9	69	theme	incomplete	1933:1942	arg1	genomes					1960:1966	incomplete or poor-quality genomes	1933:1966	incomplete or poor-quality genomes	1933:1966	The failure to assign a KL or OCL type may indicate incomplete or poor-quality genomes.
32118530	8	70	theme	KL	1730:1731	arg1	majority					1711:1718	the majority	1707:1718	the majority of common KL and OCL types	1707:1745	Among the genomes retrieved from NCBI, Kaptive detected known KL and OCL in 87 and 90 % of genomes, respectively, indicating that the majority of common KL and OCL types are captured within the databases; 13 of the 92 KL in the database were not detected in any publicly available whole genome assembly.
32118530	4	71	from	available	686:694	arg1	sequences					712:720	whole genome sequences	699:720	whole genome sequences	699:720	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	8	72	theme	whole	1858:1862	arg1	assembly					1871:1878	any publicly available whole genome assembly	1835:1878	any publicly available whole genome assembly	1835:1878	Among the genomes retrieved from NCBI, Kaptive detected known KL and OCL in 87 and 90 % of genomes, respectively, indicating that the majority of common KL and OCL types are captured within the databases; 13 of the 92 KL in the database were not detected in any publicly available whole genome assembly.
32118530	2	73	from	Variation	379:387	arg1	structure					412:420	structure	412:420	structure	412:420	Variation in the composition and structure of capsular polysaccharide (CPS), a critical determinant of virulence and phage susceptibility, makes it an attractive epidemiological marker.
32118530	2	73	from	Variation	379:387	arg1	composition					396:406	composition	396:406	composition	396:406	Variation in the composition and structure of capsular polysaccharide (CPS), a critical determinant of virulence and phage susceptibility, makes it an attractive epidemiological marker.
32118530	11	74	theme	multilocus	2053:2062	arg1	typing					2073:2078	multilocus sequence typing	2053:2078	multilocus sequence typing (Institut Pasteur scheme)	2053:2104	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	11	74	theme	multilocus	2053:2062	arg1	scheme					2098:2103	Institut Pasteur scheme	2081:2103	Institut Pasteur scheme	2081:2103	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	4	75	theme	untapped	665:672	arg1	information					674:684	the untapped information	661:684	the untapped information available in whole genome sequences	661:720	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	4	76	theme	outer	956:960	arg1	biosynthesis					967:978	outer core biosynthesis	956:978	outer core biosynthesis (OC locus)	956:989	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	4	76	theme	outer	956:960	arg1	locus					984:988	OC locus	981:988	OC locus	981:988	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	6	77	theme	in	1186:1187	arg1	typing					1196:1201	in silico typing	1186:1201	in silico typing of bacterial surface polysaccharide loci	1186:1242	These databases are compatible with Kaptive, a tool for in silico typing of bacterial surface polysaccharide loci, and their utility was validated using (a) >630 assembled A. baumannii draft genomes for which the KL and OCL regions had been previously typed manually, and (b) 3386 A. baumannii genome assemblies downloaded from NCBI.
32118530	12	78	theme	https	2465:2469	arg1	//github.com/katholt/Kaptive					2471:2498	https://github.com/katholt/Kaptive	2465:2498	https://github.com/katholt/Kaptive	2465:2498	The databases are available at https://github.com/katholt/Kaptive and will be updated as further locus types become available.
32118530	4	79	theme	available	686:694	arg1	information					674:684	the untapped information	661:684	the untapped information available in whole genome sequences	661:720	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	4	80	theme	second	902:907	arg1	database					909:916	a second database	900:916	a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus)	900:989	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	8	81	from	KL	1795:1796	arg1	database					1805:1812	the database	1801:1812	the database	1801:1812	Among the genomes retrieved from NCBI, Kaptive detected known KL and OCL in 87 and 90 % of genomes, respectively, indicating that the majority of common KL and OCL types are captured within the databases; 13 of the 92 KL in the database were not detected in any publicly available whole genome assembly.
32118530	11	82	theme	Institut	2081:2088	arg1	typing					2073:2078	multilocus sequence typing	2053:2078	multilocus sequence typing (Institut Pasteur scheme)	2053:2104	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	11	82	theme	Institut	2081:2088	arg1	scheme					2098:2103	Institut Pasteur scheme	2081:2103	Institut Pasteur scheme	2081:2103	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	4	83	theme	better	641:646	arg1	advantage					648:656	better advantage	641:656	better advantage of the untapped information available in whole genome sequences	641:720	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	1	84	theme	Multiply	211:218	arg1	infections					265:274	Multiply antibiotic-resistant Acinetobacter baumannii infections	211:274	Multiply antibiotic-resistant Acinetobacter baumannii infections	211:274	Multiply antibiotic-resistant Acinetobacter baumannii infections are a global public health concern and accurate tracking of the spread of specific lineages is needed.
32118530	1	84	theme	Multiply	211:218	arg1	concern					303:309	a global public health concern	280:309	a global public health concern	280:309	Multiply antibiotic-resistant Acinetobacter baumannii infections are a global public health concern and accurate tracking of the spread of specific lineages is needed.
32118530	11	85	theme	GC2	2279:2281	arg1	ST2					2272:2274	ST2	2272:2274	ST2	2272:2274	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	11	85	theme	GC2	2279:2281	arg1	GC1					2264:2266	GC1	2264:2266	GC1	2264:2266	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	8	86	located	detected	1823:1830	arg2	13					1782:1783	13	1782:1783	13	1782:1783	Among the genomes retrieved from NCBI, Kaptive detected known KL and OCL in 87 and 90 % of genomes, respectively, indicating that the majority of common KL and OCL types are captured within the databases; 13 of the 92 KL in the database were not detected in any publicly available whole genome assembly.
32118530	8	86	located	detected	1823:1830	arg1	assembly					1871:1878	any publicly available whole genome assembly	1835:1878	any publicly available whole genome assembly	1835:1878	Among the genomes retrieved from NCBI, Kaptive detected known KL and OCL in 87 and 90 % of genomes, respectively, indicating that the majority of common KL and OCL types are captured within the databases; 13 of the 92 KL in the database were not detected in any publicly available whole genome assembly.
32118530	8	86	located	detected	1823:1830	arg2	KL					1795:1796	the 92 KL	1788:1796	the 92 KL in the database	1788:1812	Among the genomes retrieved from NCBI, Kaptive detected known KL and OCL in 87 and 90 % of genomes, respectively, indicating that the majority of common KL and OCL types are captured within the databases; 13 of the 92 KL in the database were not detected in any publicly available whole genome assembly.
32118530	5	87	theme	OCL	1036:1038	arg1	number					1040:1045	OCL number	1036:1045	OCL number	1036:1045	Each entry has been assigned a unique KL or OCL number, and is fully annotated using a simple, transparent and standardized nomenclature.
32118530	11	88	theme	other	2306:2310	arg1	clones					2312:2317	other clones	2306:2317	other clones comprising >20 isolates each (ST10, ST25, and ST140)	2306:2370	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	11	89	theme	loci	2428:2431	arg1	replacement					2407:2417	extensive within-clone replacement	2384:2417	extensive within-clone replacement of these loci	2384:2431	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	0	90	theme	curated	158:164	arg1	databases					176:184	curated reference databases	158:184	curated reference databases compatible with Kaptive	158:208	Identification of Acinetobacter baumannii loci for capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive.
32118530	7	91	theme	typed	1485:1489	arg1	genomes					1491:1497	the previously typed genomes	1470:1497	the previously typed genomes	1470:1497	Among the previously typed genomes, Kaptive was able to confidently assign KL and OCL types with 100 % accuracy.
32118530	6	92	theme	draft	1315:1319	arg1	genomes					1321:1327	A. baumannii draft genomes	1302:1327	A. baumannii draft genomes for which the KL and OCL regions had been previously typed manually	1302:1395	These databases are compatible with Kaptive, a tool for in silico typing of bacterial surface polysaccharide loci, and their utility was validated using (a) >630 assembled A. baumannii draft genomes for which the KL and OCL regions had been previously typed manually, and (b) 3386 A. baumannii genome assemblies downloaded from NCBI.
32118530	10	93	theme	novel	1986:1990	arg1	variants					1992:1999	further novel variants	1978:1999	further novel variants	1978:1999	However, further novel variants may remain to be documented.
32118530	4	94	theme	whole	699:703	arg1	sequences					712:720	whole genome sequences	699:720	whole genome sequences	699:720	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	11	95	dep	type	2177:2180	arg1	ST					2183:2184	ST	2183:2184	ST	2183:2184	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	2	96	theme	attractive	530:539	arg1	marker					557:562	an attractive epidemiological marker	527:562	an attractive epidemiological marker	527:562	Variation in the composition and structure of capsular polysaccharide (CPS), a critical determinant of virulence and phage susceptibility, makes it an attractive epidemiological marker.
32118530	6	97	theme	A.	1302:1303	arg1	genomes					1321:1327	A. baumannii draft genomes	1302:1327	A. baumannii draft genomes for which the KL and OCL regions had been previously typed manually	1302:1395	These databases are compatible with Kaptive, a tool for in silico typing of bacterial surface polysaccharide loci, and their utility was validated using (a) >630 assembled A. baumannii draft genomes for which the KL and OCL regions had been previously typed manually, and (b) 3386 A. baumannii genome assemblies downloaded from NCBI.
32118530	1	98	theme	lineages	359:366	arg1	spread					340:345	the spread	336:345	the spread of specific lineages	336:366	Multiply antibiotic-resistant Acinetobacter baumannii infections are a global public health concern and accurate tracking of the spread of specific lineages is needed.
32118530	2	99	theme	phage	496:500	arg1	susceptibility					502:515	phage susceptibility	496:515	phage susceptibility	496:515	Variation in the composition and structure of capsular polysaccharide (CPS), a critical determinant of virulence and phage susceptibility, makes it an attractive epidemiological marker.
32118530	11	100	theme	single	2161:2166	arg1	type					2177:2180	a single sequence type	2159:2180	a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2	2159:2281	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	0	101	theme	outer	104:108	arg1	synthesis					121:129	capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis	51:129	capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive	51:208	Identification of Acinetobacter baumannii loci for capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive.
32118530	2	102	dep	composition	396:406	arg1	the					392:394	the	392:394	the	392:394	Variation in the composition and structure of capsular polysaccharide (CPS), a critical determinant of virulence and phage susceptibility, makes it an attractive epidemiological marker.
32118530	6	103	dep	>630	1287:1290	arg1	a					1284:1284	a	1284:1284	a	1284:1284	These databases are compatible with Kaptive, a tool for in silico typing of bacterial surface polysaccharide loci, and their utility was validated using (a) >630 assembled A. baumannii draft genomes for which the KL and OCL regions had been previously typed manually, and (b) 3386 A. baumannii genome assemblies downloaded from NCBI.
32118530	4	104	theme	gene	925:928	arg1	clusters					930:937	12 gene clusters	922:937	12 gene clusters at the locus for outer core biosynthesis (OC locus)	922:989	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	11	105	theme	ST2	2272:2274	arg1	ST1					2257:2259	ST1	2257:2259	ST1 of GC1 and ST2 of GC2	2257:2281	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	11	105	theme	ST2	2272:2274	arg1	clones					2249:2254	the two predominant globally distributed clones	2208:2254	the two predominant globally distributed clones	2208:2254	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	6	106	dep	A.	1302:1303	arg1	baumannii					1305:1313	baumannii	1305:1313	baumannii	1305:1313	These databases are compatible with Kaptive, a tool for in silico typing of bacterial surface polysaccharide loci, and their utility was validated using (a) >630 assembled A. baumannii draft genomes for which the KL and OCL regions had been previously typed manually, and (b) 3386 A. baumannii genome assemblies downloaded from NCBI.
32118530	11	107	theme	GC1	2264:2266	arg1	ST1					2257:2259	ST1	2257:2259	ST1 of GC1 and ST2 of GC2	2257:2281	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	11	107	theme	GC1	2264:2266	arg1	clones					2249:2254	the two predominant globally distributed clones	2208:2254	the two predominant globally distributed clones	2208:2254	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	4	108	theme	available	783:791	arg1	clusters					798:805	92 publicly available gene clusters	771:805	92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus)	771:893	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	4	109	from	database	759:766	arg1	locus					814:818	the locus	810:818	the locus encoding proteins responsible for biosynthesis and export of CPS (K locus)	810:893	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	7	110	theme	%	1565:1565	arg1	accuracy					1567:1574	100 % accuracy	1561:1574	100 % accuracy	1561:1574	Among the previously typed genomes, Kaptive was able to confidently assign KL and OCL types with 100 % accuracy.
32118530	11	111	theme	type	2177:2180	arg1	collections					2144:2154	collections	2144:2154	collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2	2144:2281	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	4	112	theme	curated	741:747	arg1	database					759:766	a curated reference database	739:766	a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus)	739:893	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	0	113	theme	baumannii	32:40	arg1	loci					42:45	Acinetobacter baumannii loci	18:45	Acinetobacter baumannii loci for capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive	18:208	Identification of Acinetobacter baumannii loci for capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive.
32118530	8	114	theme	available	1848:1856	arg1	assembly					1871:1878	any publicly available whole genome assembly	1835:1878	any publicly available whole genome assembly	1835:1878	Among the genomes retrieved from NCBI, Kaptive detected known KL and OCL in 87 and 90 % of genomes, respectively, indicating that the majority of common KL and OCL types are captured within the databases; 13 of the 92 KL in the database were not detected in any publicly available whole genome assembly.
32118530	7	115	theme	OCL	1546:1548	arg1	types					1550:1554	OCL types	1546:1554	OCL types	1546:1554	Among the previously typed genomes, Kaptive was able to confidently assign KL and OCL types with 100 % accuracy.
32118530	2	116	theme	virulence	482:490	arg1	polysaccharide					434:447	capsular polysaccharide	425:447	capsular polysaccharide (CPS)	425:453	Variation in the composition and structure of capsular polysaccharide (CPS), a critical determinant of virulence and phage susceptibility, makes it an attractive epidemiological marker.
32118530	2	116	theme	virulence	482:490	arg1	determinant					467:477	a critical determinant	456:477	a critical determinant of virulence and phage susceptibility	456:515	Variation in the composition and structure of capsular polysaccharide (CPS), a critical determinant of virulence and phage susceptibility, makes it an attractive epidemiological marker.
32118530	11	117	theme	extensive	2384:2392	arg1	replacement					2407:2417	extensive within-clone replacement	2384:2417	extensive within-clone replacement of these loci	2384:2431	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	8	118	theme	KL	1795:1796	arg1	KL					1795:1796	the 92 KL	1788:1796	the 92 KL in the database	1788:1812	Among the genomes retrieved from NCBI, Kaptive detected known KL and OCL in 87 and 90 % of genomes, respectively, indicating that the majority of common KL and OCL types are captured within the databases; 13 of the 92 KL in the database were not detected in any publicly available whole genome assembly.
32118530	8	118	theme	KL	1795:1796	arg1	13					1782:1783	13	1782:1783	13	1782:1783	Among the genomes retrieved from NCBI, Kaptive detected known KL and OCL in 87 and 90 % of genomes, respectively, indicating that the majority of common KL and OCL types are captured within the databases; 13 of the 92 KL in the database were not detected in any publicly available whole genome assembly.
32118530	2	119	theme	capsular	425:432	arg1	polysaccharide					434:447	capsular polysaccharide	425:447	capsular polysaccharide (CPS)	425:453	Variation in the composition and structure of capsular polysaccharide (CPS), a critical determinant of virulence and phage susceptibility, makes it an attractive epidemiological marker.
32118530	2	119	theme	capsular	425:432	arg1	determinant					467:477	a critical determinant	456:477	a critical determinant of virulence and phage susceptibility	456:515	Variation in the composition and structure of capsular polysaccharide (CPS), a critical determinant of virulence and phage susceptibility, makes it an attractive epidemiological marker.
32118530	2	119	theme	capsular	425:432	arg1	CPS					450:452	CPS	450:452	CPS	450:452	Variation in the composition and structure of capsular polysaccharide (CPS), a critical determinant of virulence and phage susceptibility, makes it an attractive epidemiological marker.
32118530	0	120	theme	polysaccharide	60:73	arg1	synthesis					121:129	capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis	51:129	capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive	51:208	Identification of Acinetobacter baumannii loci for capsular polysaccharide (KL) and lipooligosaccharide outer core (OCL) synthesis in genome assemblies using curated reference databases compatible with Kaptive.
32118530	12	121	theme	further	2523:2529	arg1	types					2537:2541	further locus types	2523:2541	further locus types	2523:2541	The databases are available at https://github.com/katholt/Kaptive and will be updated as further locus types become available.
32118530	4	122	theme	responsible	838:848	arg1	proteins					829:836	proteins	829:836	proteins responsible for biosynthesis and export of CPS (K locus)	829:893	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	4	123	theme	clusters	798:805	arg1	database					759:766	a curated reference database	739:766	a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus)	739:893	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	4	123	theme	clusters	798:805	arg1	database					909:916	a second database	900:916	a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus)	900:989	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
32118530	1	124	theme	baumannii	255:263	arg1	infections					265:274	Multiply antibiotic-resistant Acinetobacter baumannii infections	211:274	Multiply antibiotic-resistant Acinetobacter baumannii infections	211:274	Multiply antibiotic-resistant Acinetobacter baumannii infections are a global public health concern and accurate tracking of the spread of specific lineages is needed.
32118530	1	124	theme	baumannii	255:263	arg1	concern					303:309	a global public health concern	280:309	a global public health concern	280:309	Multiply antibiotic-resistant Acinetobacter baumannii infections are a global public health concern and accurate tracking of the spread of specific lineages is needed.
32118530	6	125	dep	in	1186:1187	arg1	silico					1189:1194	silico	1189:1194	silico	1189:1194	These databases are compatible with Kaptive, a tool for in silico typing of bacterial surface polysaccharide loci, and their utility was validated using (a) >630 assembled A. baumannii draft genomes for which the KL and OCL regions had been previously typed manually, and (b) 3386 A. baumannii genome assemblies downloaded from NCBI.
32118530	11	126	theme	multiple	2115:2122	arg1	KL					2124:2125	multiple KL and OCL types	2115:2139	KL	2124:2125	Combining outputs with multilocus sequence typing (Institut Pasteur scheme) revealed multiple KL and OCL types in collections of a single sequence type (ST) representing each of the two predominant globally distributed clones, ST1 of GC1 and ST2 of GC2, and in collections of other clones comprising >20 isolates each (ST10, ST25, and ST140), indicating extensive within-clone replacement of these loci.
32118530	12	127	from	//github.com/katholt/Kaptive	2471:2498	arg1	available					2452:2460	available	2452:2460	available	2452:2460	The databases are available at https://github.com/katholt/Kaptive and will be updated as further locus types become available.
32118530	12	127	from	//github.com/katholt/Kaptive	2471:2498	arg1	databases					2438:2446	The databases	2434:2446	The databases	2434:2446	The databases are available at https://github.com/katholt/Kaptive and will be updated as further locus types become available.
32118530	4	128	from	locus	946:950	arg1	clusters					930:937	12 gene clusters	922:937	12 gene clusters at the locus for outer core biosynthesis (OC locus)	922:989	To take better advantage of the untapped information available in whole genome sequences, we have created a curated reference database of 92 publicly available gene clusters at the locus encoding proteins responsible for biosynthesis and export of CPS (K locus), and a second database for 12 gene clusters at the locus for outer core biosynthesis (OC locus).
34628317	3	0	theme	removal	532:538	arg1	capacity					540:547	its lead (Pb2+) removal capacity	516:547	its lead (Pb2+) removal capacity from aqueous solution	516:569	In the present study, a magnetic chitosan-palygorskite (MCP) nanocomposite was prepared through a straight-forward one pot synthesis approach to evaluate its lead (Pb2+) removal capacity from aqueous solution.
34628317	4	1	theme	MCP	649:651	arg1	composite					653:661	the newly-developed MCP composite	629:661	the newly-developed MCP composite	629:661	The nano-architectural and physicochemical properties of the newly-developed MCP composite were described via micro- and nano-morphological analyses, and crystallinity, surface porosity and magnetic susceptibility measurements.
34628317	7	2	theme	Thermodynamic	1163:1175	arg1	analysis					1177:1184	Thermodynamic analysis	1163:1184	Thermodynamic analysis	1163:1184	Thermodynamic analysis suggested that the adsorption process at low Pb2+ concentration was controlled by chemisorption, whereas that at high Pb2+ concentration was dominated by physical adsorption.
34628317	5	3	theme	data	923:926	arg1	agreement					897:905	a good agreement	890:905	a good agreement of experimental data	890:926	The MCP nanocomposite was capable to remove up to 58.5 mg Pb2+ g-1 of MCP from water with a good agreement of experimental data to the Langmuir isotherm model (R2 = 0.98).
34628317	5	4	with	water	879:883	arg1	agreement					897:905	a good agreement	890:905	a good agreement of experimental data	890:926	The MCP nanocomposite was capable to remove up to 58.5 mg Pb2+ g-1 of MCP from water with a good agreement of experimental data to the Langmuir isotherm model (R2 = 0.98).
34628317	2	5	theme	preparation	343:353	arg1	steps					355:359	tedious preparation steps	335:359	tedious preparation steps	335:359	However, the synthesis of magnetic composites involving multiple components frequently involves tedious preparation steps.
34628317	1	6	theme	efficient	196:204	arg1	treatment					206:214	efficient treatment	196:214	efficient treatment of contaminated water	196:236	Development of polymeric magnetic adsorbents is a promising approach to obtain efficient treatment of contaminated water.
34628317	5	7	theme	good	892:895	arg1	agreement					897:905	a good agreement	890:905	a good agreement of experimental data	890:926	The MCP nanocomposite was capable to remove up to 58.5 mg Pb2+ g-1 of MCP from water with a good agreement of experimental data to the Langmuir isotherm model (R2 = 0.98).
34628317	4	8	theme	susceptibility	771:784	arg1	measurements					786:797	magnetic susceptibility measurements	762:797	magnetic susceptibility measurements	762:797	The nano-architectural and physicochemical properties of the newly-developed MCP composite were described via micro- and nano-morphological analyses, and crystallinity, surface porosity and magnetic susceptibility measurements.
34628317	10	9	theme	structural	1765:1774	arg1	properties					1776:1785	the enhanced structural properties	1752:1785	the enhanced structural properties of the easily-prepared nanocomposite which holds outstanding potential to be used as an inexpensive and green adsorbent for remediating Pb2+ contaminated water	1752:1945	These findings highlight the enhanced structural properties of the easily-prepared nanocomposite which holds outstanding potential to be used as an inexpensive and green adsorbent for remediating Pb2+ contaminated water.
34628317	8	10	theme	X-ray	1361:1365	arg1	photoelectron					1367:1379	X-ray photoelectron	1361:1379	X-ray photoelectron	1361:1379	X-ray photoelectron and Fourier transform infrared spectroscopy results suggested that the Pb adsorption on MCP was governed by surface complexation and chemical reduction mechanisms.
34628317	9	11	theme	%	1585:1585	arg1	capacity					1603:1610	82% Pb2+ adsorption capacity	1583:1610	82% Pb2+ adsorption capacity	1583:1610	During regeneration, the MCP retained 82% Pb2+ adsorption capacity following four adsorption-desorption cycles with ease to recover the adsorbent using its strong magnetic property.
34628317	9	12	theme	adsorption	1592:1601	arg1	capacity					1603:1610	82% Pb2+ adsorption capacity	1583:1610	82% Pb2+ adsorption capacity	1583:1610	During regeneration, the MCP retained 82% Pb2+ adsorption capacity following four adsorption-desorption cycles with ease to recover the adsorbent using its strong magnetic property.
34628317	6	13	theme	Pb2+	976:979	arg1	process					992:998	The Pb2+ adsorption process	972:998	The Pb2+ adsorption process on MCP	972:1005	The Pb2+ adsorption process on MCP was a multistep diffusion-controlled phenomenon evidenced by the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96).
34628317	6	13	theme	Pb2+	976:979	arg1	phenomenon					1044:1053	a multistep diffusion-controlled phenomenon	1011:1053	a multistep diffusion-controlled phenomenon evidenced by the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96)	1011:1160	The Pb2+ adsorption process on MCP was a multistep diffusion-controlled phenomenon evidenced by the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96).
34628317	0	14	from	water	110:114	arg1	removal					89:95	efficient removal	79:95	efficient removal of lead from water	79:114	Facile one pot preparation of magnetic chitosan-palygorskite nanocomposite for efficient removal of lead from water.
34628317	4	15	theme	surface	741:747	arg1	porosity					749:756	surface porosity	741:756	surface porosity	741:756	The nano-architectural and physicochemical properties of the newly-developed MCP composite were described via micro- and nano-morphological analyses, and crystallinity, surface porosity and magnetic susceptibility measurements.
34628317	2	16	theme	magnetic	265:272	arg1	composites					274:283	magnetic composites	265:283	magnetic composites involving multiple components	265:313	However, the synthesis of magnetic composites involving multiple components frequently involves tedious preparation steps.
34628317	1	17	theme	magnetic	142:149	arg1	adsorbents					151:160	polymeric magnetic adsorbents	132:160	polymeric magnetic adsorbents	132:160	Development of polymeric magnetic adsorbents is a promising approach to obtain efficient treatment of contaminated water.
34628317	7	18	theme	Pb2+	1304:1307	arg1	concentration					1309:1321	high Pb2+ concentration	1299:1321	high Pb2+ concentration	1299:1321	Thermodynamic analysis suggested that the adsorption process at low Pb2+ concentration was controlled by chemisorption, whereas that at high Pb2+ concentration was dominated by physical adsorption.
34628317	5	19	theme	isotherm	944:951	arg1	model					953:957	the Langmuir isotherm model	931:957	the Langmuir isotherm model (R2 = 0.98)	931:969	The MCP nanocomposite was capable to remove up to 58.5 mg Pb2+ g-1 of MCP from water with a good agreement of experimental data to the Langmuir isotherm model (R2 = 0.98).
34628317	5	19	theme	isotherm	944:951	arg1	R2 = 0.98					960:968	R2 = 0.98	960:968	R2 = 0.98	960:968	The MCP nanocomposite was capable to remove up to 58.5 mg Pb2+ g-1 of MCP from water with a good agreement of experimental data to the Langmuir isotherm model (R2 = 0.98).
34628317	9	20	with	cycles	1649:1654	arg1	ease					1661:1664	ease	1661:1664	ease	1661:1664	During regeneration, the MCP retained 82% Pb2+ adsorption capacity following four adsorption-desorption cycles with ease to recover the adsorbent using its strong magnetic property.
34628317	4	21	theme	magnetic	762:769	arg1	measurements					786:797	magnetic susceptibility measurements	762:797	magnetic susceptibility measurements	762:797	The nano-architectural and physicochemical properties of the newly-developed MCP composite were described via micro- and nano-morphological analyses, and crystallinity, surface porosity and magnetic susceptibility measurements.
34628317	3	22	from	solution	562:569	arg1	capacity					540:547	its lead (Pb2+) removal capacity	516:547	its lead (Pb2+) removal capacity from aqueous solution	516:569	In the present study, a magnetic chitosan-palygorskite (MCP) nanocomposite was prepared through a straight-forward one pot synthesis approach to evaluate its lead (Pb2+) removal capacity from aqueous solution.
34628317	1	23	theme	promising	167:175	arg1	approach					177:184	a promising approach	165:184	a promising approach to obtain efficient treatment of contaminated water	165:236	Development of polymeric magnetic adsorbents is a promising approach to obtain efficient treatment of contaminated water.
34628317	1	23	theme	promising	167:175	arg1	Development					117:127	Development	117:127	Development of polymeric magnetic adsorbents	117:160	Development of polymeric magnetic adsorbents is a promising approach to obtain efficient treatment of contaminated water.
34628317	0	24	theme	lead	100:103	arg1	removal					89:95	efficient removal	79:95	efficient removal of lead from water	79:114	Facile one pot preparation of magnetic chitosan-palygorskite nanocomposite for efficient removal of lead from water.
34628317	6	25	theme	diffusion-controlled	1023:1042	arg1	process					992:998	The Pb2+ adsorption process	972:998	The Pb2+ adsorption process on MCP	972:1005	The Pb2+ adsorption process on MCP was a multistep diffusion-controlled phenomenon evidenced by the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96).
34628317	6	25	theme	diffusion-controlled	1023:1042	arg1	phenomenon					1044:1053	a multistep diffusion-controlled phenomenon	1011:1053	a multistep diffusion-controlled phenomenon evidenced by the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96)	1011:1160	The Pb2+ adsorption process on MCP was a multistep diffusion-controlled phenomenon evidenced by the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96).
34628317	7	26	from	concentration	1236:1248	arg1	process					1216:1222	the adsorption process	1201:1222	the adsorption process at low Pb2+ concentration	1201:1248	Thermodynamic analysis suggested that the adsorption process at low Pb2+ concentration was controlled by chemisorption, whereas that at high Pb2+ concentration was dominated by physical adsorption.
34628317	10	27	dep	inexpensive	1875:1885	arg1	adsorbent					1897:1905	adsorbent	1897:1905	adsorbent	1897:1905	These findings highlight the enhanced structural properties of the easily-prepared nanocomposite which holds outstanding potential to be used as an inexpensive and green adsorbent for remediating Pb2+ contaminated water.
34628317	0	28	theme	magnetic	30:37	arg1	chitosan-palygorskite					39:59	magnetic chitosan-palygorskite	30:59	magnetic chitosan-palygorskite	30:59	Facile one pot preparation of magnetic chitosan-palygorskite nanocomposite for efficient removal of lead from water.
34628317	3	29	theme	MCP	418:420	arg1	nanocomposite					423:435	a magnetic chitosan-palygorskite (MCP) nanocomposite	384:435	a magnetic chitosan-palygorskite (MCP) nanocomposite	384:435	In the present study, a magnetic chitosan-palygorskite (MCP) nanocomposite was prepared through a straight-forward one pot synthesis approach to evaluate its lead (Pb2+) removal capacity from aqueous solution.
34628317	0	30	theme	efficient	79:87	arg1	removal					89:95	efficient removal	79:95	efficient removal of lead from water	79:114	Facile one pot preparation of magnetic chitosan-palygorskite nanocomposite for efficient removal of lead from water.
34628317	0	31	theme	Facile	0:5	arg1	preparation					15:25	Facile one pot preparation	0:25	Facile one pot preparation of magnetic chitosan-palygorskite	0:59	Facile one pot preparation of magnetic chitosan-palygorskite nanocomposite for efficient removal of lead from water.
34628317	6	32	theme	intra-particle	1119:1132	arg1	R2 = 0.96					1151:1159	R2 = 0.96	1151:1159	R2 = 0.96	1151:1159	The Pb2+ adsorption process on MCP was a multistep diffusion-controlled phenomenon evidenced by the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96).
34628317	6	32	theme	intra-particle	1119:1132	arg1	model					1144:1148	the intra-particle diffusion model	1115:1148	the intra-particle diffusion model (R2 = 0.96)	1115:1160	The Pb2+ adsorption process on MCP was a multistep diffusion-controlled phenomenon evidenced by the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96).
34628317	3	33	theme	chitosan-palygorskite	395:415	arg1	nanocomposite					423:435	a magnetic chitosan-palygorskite (MCP) nanocomposite	384:435	a magnetic chitosan-palygorskite (MCP) nanocomposite	384:435	In the present study, a magnetic chitosan-palygorskite (MCP) nanocomposite was prepared through a straight-forward one pot synthesis approach to evaluate its lead (Pb2+) removal capacity from aqueous solution.
34628317	4	34	theme	nano-architectural	576:593	arg1	properties					615:624	The nano-architectural and physicochemical properties	572:624	The nano-architectural and physicochemical properties of the newly-developed MCP composite	572:661	The nano-architectural and physicochemical properties of the newly-developed MCP composite were described via micro- and nano-morphological analyses, and crystallinity, surface porosity and magnetic susceptibility measurements.
34628317	0	35	theme	pot	11:13	arg1	preparation					15:25	Facile one pot preparation	0:25	Facile one pot preparation of magnetic chitosan-palygorskite	0:59	Facile one pot preparation of magnetic chitosan-palygorskite nanocomposite for efficient removal of lead from water.
34628317	3	36	theme	aqueous	554:560	arg1	solution					562:569	aqueous solution	554:569	aqueous solution	554:569	In the present study, a magnetic chitosan-palygorskite (MCP) nanocomposite was prepared through a straight-forward one pot synthesis approach to evaluate its lead (Pb2+) removal capacity from aqueous solution.
34628317	9	37	theme	strong	1701:1706	arg1	property					1717:1724	its strong magnetic property	1697:1724	its strong magnetic property	1697:1724	During regeneration, the MCP retained 82% Pb2+ adsorption capacity following four adsorption-desorption cycles with ease to recover the adsorbent using its strong magnetic property.
34628317	1	38	theme	adsorbents	151:160	arg1	Development					117:127	Development	117:127	Development of polymeric magnetic adsorbents	117:160	Development of polymeric magnetic adsorbents is a promising approach to obtain efficient treatment of contaminated water.
34628317	1	38	theme	adsorbents	151:160	arg1	approach					177:184	a promising approach	165:184	a promising approach to obtain efficient treatment of contaminated water	165:236	Development of polymeric magnetic adsorbents is a promising approach to obtain efficient treatment of contaminated water.
34628317	4	39	theme	physicochemical	599:613	arg1	properties					615:624	The nano-architectural and physicochemical properties	572:624	The nano-architectural and physicochemical properties of the newly-developed MCP composite	572:661	The nano-architectural and physicochemical properties of the newly-developed MCP composite were described via micro- and nano-morphological analyses, and crystallinity, surface porosity and magnetic susceptibility measurements.
34628317	10	40	theme	nanocomposite	1810:1822	arg1	properties					1776:1785	the enhanced structural properties	1752:1785	the enhanced structural properties of the easily-prepared nanocomposite which holds outstanding potential to be used as an inexpensive and green adsorbent for remediating Pb2+ contaminated water	1752:1945	These findings highlight the enhanced structural properties of the easily-prepared nanocomposite which holds outstanding potential to be used as an inexpensive and green adsorbent for remediating Pb2+ contaminated water.
34628317	8	41	theme	surface	1489:1495	arg1	complexation					1497:1508	surface complexation	1489:1508	surface complexation	1489:1508	X-ray photoelectron and Fourier transform infrared spectroscopy results suggested that the Pb adsorption on MCP was governed by surface complexation and chemical reduction mechanisms.
34628317	3	42	theme	synthesis	485:493	arg1	approach					495:502	a straight-forward one pot synthesis approach	458:502	a straight-forward one pot synthesis approach to evaluate its lead (Pb2+) removal capacity from aqueous solution	458:569	In the present study, a magnetic chitosan-palygorskite (MCP) nanocomposite was prepared through a straight-forward one pot synthesis approach to evaluate its lead (Pb2+) removal capacity from aqueous solution.
34628317	5	43	theme	Pb2+	858:861	arg1	g-1					863:865	58.5 mg Pb2+ g-1	850:865	58.5 mg Pb2+ g-1 of MCP	850:872	The MCP nanocomposite was capable to remove up to 58.5 mg Pb2+ g-1 of MCP from water with a good agreement of experimental data to the Langmuir isotherm model (R2 = 0.98).
34628317	7	44	theme	low	1227:1229	arg1	concentration					1236:1248	low Pb2+ concentration	1227:1248	low Pb2+ concentration	1227:1248	Thermodynamic analysis suggested that the adsorption process at low Pb2+ concentration was controlled by chemisorption, whereas that at high Pb2+ concentration was dominated by physical adsorption.
34628317	6	45	theme	adsorption	1096:1105	arg1	data					1107:1110	kinetic adsorption data	1088:1110	kinetic adsorption data	1088:1110	The Pb2+ adsorption process on MCP was a multistep diffusion-controlled phenomenon evidenced by the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96).
34628317	0	46	theme	chitosan-palygorskite	39:59	arg1	preparation					15:25	Facile one pot preparation	0:25	Facile one pot preparation of magnetic chitosan-palygorskite	0:59	Facile one pot preparation of magnetic chitosan-palygorskite nanocomposite for efficient removal of lead from water.
34628317	8	47	theme	reduction	1523:1531	arg1	mechanisms					1533:1542	chemical reduction mechanisms	1514:1542	chemical reduction mechanisms	1514:1542	X-ray photoelectron and Fourier transform infrared spectroscopy results suggested that the Pb adsorption on MCP was governed by surface complexation and chemical reduction mechanisms.
34628317	8	48	dep	Fourier	1385:1391	arg1	transform					1393:1401	transform	1393:1401	transform infrared spectroscopy	1393:1423	X-ray photoelectron and Fourier transform infrared spectroscopy results suggested that the Pb adsorption on MCP was governed by surface complexation and chemical reduction mechanisms.
34628317	9	49	theme	adsorption-desorption	1627:1647	arg1	cycles					1649:1654	four adsorption-desorption cycles	1622:1654	four adsorption-desorption cycles with ease to recover the adsorbent using its strong magnetic property	1622:1724	During regeneration, the MCP retained 82% Pb2+ adsorption capacity following four adsorption-desorption cycles with ease to recover the adsorbent using its strong magnetic property.
34628317	4	50	theme	composite	653:661	arg1	properties					615:624	The nano-architectural and physicochemical properties	572:624	The nano-architectural and physicochemical properties of the newly-developed MCP composite	572:661	The nano-architectural and physicochemical properties of the newly-developed MCP composite were described via micro- and nano-morphological analyses, and crystallinity, surface porosity and magnetic susceptibility measurements.
34628317	10	51	dep	contaminated	1928:1939	arg1	Pb2+					1923:1926	Pb2+	1923:1926	Pb2+	1923:1926	These findings highlight the enhanced structural properties of the easily-prepared nanocomposite which holds outstanding potential to be used as an inexpensive and green adsorbent for remediating Pb2+ contaminated water.
34628317	4	52	theme	newly-developed	633:647	arg1	composite					653:661	the newly-developed MCP composite	629:661	the newly-developed MCP composite	629:661	The nano-architectural and physicochemical properties of the newly-developed MCP composite were described via micro- and nano-morphological analyses, and crystallinity, surface porosity and magnetic susceptibility measurements.
34628317	2	53	theme	multiple	295:302	arg1	components					304:313	multiple components	295:313	multiple components	295:313	However, the synthesis of magnetic composites involving multiple components frequently involves tedious preparation steps.
34628317	5	54	theme	experimental	910:921	arg1	data					923:926	experimental data	910:926	experimental data	910:926	The MCP nanocomposite was capable to remove up to 58.5 mg Pb2+ g-1 of MCP from water with a good agreement of experimental data to the Langmuir isotherm model (R2 = 0.98).
34628317	6	55	theme	multistep	1013:1021	arg1	process					992:998	The Pb2+ adsorption process	972:998	The Pb2+ adsorption process on MCP	972:1005	The Pb2+ adsorption process on MCP was a multistep diffusion-controlled phenomenon evidenced by the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96).
34628317	6	55	theme	multistep	1013:1021	arg1	phenomenon					1044:1053	a multistep diffusion-controlled phenomenon	1011:1053	a multistep diffusion-controlled phenomenon evidenced by the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96)	1011:1160	The Pb2+ adsorption process on MCP was a multistep diffusion-controlled phenomenon evidenced by the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96).
34628317	2	56	theme	tedious	335:341	arg1	steps					355:359	tedious preparation steps	335:359	tedious preparation steps	335:359	However, the synthesis of magnetic composites involving multiple components frequently involves tedious preparation steps.
34628317	10	57	theme	contaminated	1928:1939	arg1	water					1941:1945	Pb2+ contaminated water	1923:1945	Pb2+ contaminated water	1923:1945	These findings highlight the enhanced structural properties of the easily-prepared nanocomposite which holds outstanding potential to be used as an inexpensive and green adsorbent for remediating Pb2+ contaminated water.
34628317	4	58	theme	micro-	682:687	arg1	analyses					712:719	micro- and nano-morphological analyses	682:719	micro- and nano-morphological analyses	682:719	The nano-architectural and physicochemical properties of the newly-developed MCP composite were described via micro- and nano-morphological analyses, and crystallinity, surface porosity and magnetic susceptibility measurements.
34628317	10	59	theme	enhanced	1756:1763	arg1	properties					1776:1785	the enhanced structural properties	1752:1785	the enhanced structural properties of the easily-prepared nanocomposite which holds outstanding potential to be used as an inexpensive and green adsorbent for remediating Pb2+ contaminated water	1752:1945	These findings highlight the enhanced structural properties of the easily-prepared nanocomposite which holds outstanding potential to be used as an inexpensive and green adsorbent for remediating Pb2+ contaminated water.
34628317	1	60	theme	contaminated	219:230	arg1	water					232:236	contaminated water	219:236	contaminated water	219:236	Development of polymeric magnetic adsorbents is a promising approach to obtain efficient treatment of contaminated water.
34628317	4	61	theme	nano-morphological	693:710	arg1	analyses					712:719	micro- and nano-morphological analyses	682:719	micro- and nano-morphological analyses	682:719	The nano-architectural and physicochemical properties of the newly-developed MCP composite were described via micro- and nano-morphological analyses, and crystallinity, surface porosity and magnetic susceptibility measurements.
34628317	9	62	theme	Pb2+	1587:1590	arg1	capacity					1603:1610	82% Pb2+ adsorption capacity	1583:1610	82% Pb2+ adsorption capacity	1583:1610	During regeneration, the MCP retained 82% Pb2+ adsorption capacity following four adsorption-desorption cycles with ease to recover the adsorbent using its strong magnetic property.
34628317	6	63	theme	adsorption	981:990	arg1	process					992:998	The Pb2+ adsorption process	972:998	The Pb2+ adsorption process on MCP	972:1005	The Pb2+ adsorption process on MCP was a multistep diffusion-controlled phenomenon evidenced by the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96).
34628317	6	63	theme	adsorption	981:990	arg1	phenomenon					1044:1053	a multistep diffusion-controlled phenomenon	1011:1053	a multistep diffusion-controlled phenomenon evidenced by the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96)	1011:1160	The Pb2+ adsorption process on MCP was a multistep diffusion-controlled phenomenon evidenced by the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96).
34628317	8	64	theme	photoelectron	1367:1379	arg1	results					1425:1431	X-ray photoelectron and Fourier transform infrared spectroscopy results	1361:1431	X-ray photoelectron and Fourier transform infrared spectroscopy results	1361:1431	X-ray photoelectron and Fourier transform infrared spectroscopy results suggested that the Pb adsorption on MCP was governed by surface complexation and chemical reduction mechanisms.
34628317	2	65	theme	composites	274:283	arg1	synthesis					252:260	the synthesis	248:260	the synthesis of magnetic composites involving multiple components	248:313	However, the synthesis of magnetic composites involving multiple components frequently involves tedious preparation steps.
34628317	1	66	theme	polymeric	132:140	arg1	adsorbents					151:160	polymeric magnetic adsorbents	132:160	polymeric magnetic adsorbents	132:160	Development of polymeric magnetic adsorbents is a promising approach to obtain efficient treatment of contaminated water.
34628317	6	67	theme	kinetic	1088:1094	arg1	data					1107:1110	kinetic adsorption data	1088:1110	kinetic adsorption data	1088:1110	The Pb2+ adsorption process on MCP was a multistep diffusion-controlled phenomenon evidenced by the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96).
34628317	8	68	theme	Fourier	1385:1391	arg1	results					1425:1431	X-ray photoelectron and Fourier transform infrared spectroscopy results	1361:1431	X-ray photoelectron and Fourier transform infrared spectroscopy results	1361:1431	X-ray photoelectron and Fourier transform infrared spectroscopy results suggested that the Pb adsorption on MCP was governed by surface complexation and chemical reduction mechanisms.
34628317	7	69	theme	high	1299:1302	arg1	concentration					1309:1321	high Pb2+ concentration	1299:1321	high Pb2+ concentration	1299:1321	Thermodynamic analysis suggested that the adsorption process at low Pb2+ concentration was controlled by chemisorption, whereas that at high Pb2+ concentration was dominated by physical adsorption.
34628317	5	70	theme	Langmuir	935:942	arg1	model					953:957	the Langmuir isotherm model	931:957	the Langmuir isotherm model (R2 = 0.98)	931:969	The MCP nanocomposite was capable to remove up to 58.5 mg Pb2+ g-1 of MCP from water with a good agreement of experimental data to the Langmuir isotherm model (R2 = 0.98).
34628317	5	70	theme	Langmuir	935:942	arg1	R2 = 0.98					960:968	R2 = 0.98	960:968	R2 = 0.98	960:968	The MCP nanocomposite was capable to remove up to 58.5 mg Pb2+ g-1 of MCP from water with a good agreement of experimental data to the Langmuir isotherm model (R2 = 0.98).
34628317	3	71	theme	present	369:375	arg1	study					377:381	the present study	365:381	the present study	365:381	In the present study, a magnetic chitosan-palygorskite (MCP) nanocomposite was prepared through a straight-forward one pot synthesis approach to evaluate its lead (Pb2+) removal capacity from aqueous solution.
34628317	8	72	theme	infrared	1403:1410	arg1	spectroscopy					1412:1423	infrared spectroscopy	1403:1423	infrared spectroscopy	1403:1423	X-ray photoelectron and Fourier transform infrared spectroscopy results suggested that the Pb adsorption on MCP was governed by surface complexation and chemical reduction mechanisms.
34628317	5	73	theme	MCP	804:806	arg1	capable					826:832	capable	826:832	capable	826:832	The MCP nanocomposite was capable to remove up to 58.5 mg Pb2+ g-1 of MCP from water with a good agreement of experimental data to the Langmuir isotherm model (R2 = 0.98).
34628317	5	73	theme	MCP	804:806	arg1	nanocomposite					808:820	The MCP nanocomposite	800:820	The MCP nanocomposite	800:820	The MCP nanocomposite was capable to remove up to 58.5 mg Pb2+ g-1 of MCP from water with a good agreement of experimental data to the Langmuir isotherm model (R2 = 0.98).
34628317	3	74	theme	magnetic	386:393	arg1	nanocomposite					423:435	a magnetic chitosan-palygorskite (MCP) nanocomposite	384:435	a magnetic chitosan-palygorskite (MCP) nanocomposite	384:435	In the present study, a magnetic chitosan-palygorskite (MCP) nanocomposite was prepared through a straight-forward one pot synthesis approach to evaluate its lead (Pb2+) removal capacity from aqueous solution.
34628317	10	75	theme	outstanding	1836:1846	arg1	potential					1848:1856	outstanding potential	1836:1856	outstanding potential to be used as an inexpensive and green adsorbent for remediating Pb2+ contaminated water	1836:1945	These findings highlight the enhanced structural properties of the easily-prepared nanocomposite which holds outstanding potential to be used as an inexpensive and green adsorbent for remediating Pb2+ contaminated water.
34628317	8	76	theme	Pb	1452:1453	arg1	adsorption					1455:1464	the Pb adsorption	1448:1464	the Pb adsorption on MCP	1448:1471	X-ray photoelectron and Fourier transform infrared spectroscopy results suggested that the Pb adsorption on MCP was governed by surface complexation and chemical reduction mechanisms.
34628317	3	77	theme	pot	481:483	arg1	approach					495:502	a straight-forward one pot synthesis approach	458:502	a straight-forward one pot synthesis approach to evaluate its lead (Pb2+) removal capacity from aqueous solution	458:569	In the present study, a magnetic chitosan-palygorskite (MCP) nanocomposite was prepared through a straight-forward one pot synthesis approach to evaluate its lead (Pb2+) removal capacity from aqueous solution.
34628317	5	78	theme	MCP	870:872	arg1	g-1					863:865	58.5 mg Pb2+ g-1	850:865	58.5 mg Pb2+ g-1 of MCP	850:872	The MCP nanocomposite was capable to remove up to 58.5 mg Pb2+ g-1 of MCP from water with a good agreement of experimental data to the Langmuir isotherm model (R2 = 0.98).
34628317	6	79	theme	diffusion	1134:1142	arg1	R2 = 0.96					1151:1159	R2 = 0.96	1151:1159	R2 = 0.96	1151:1159	The Pb2+ adsorption process on MCP was a multistep diffusion-controlled phenomenon evidenced by the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96).
34628317	6	79	theme	diffusion	1134:1142	arg1	model					1144:1148	the intra-particle diffusion model	1115:1148	the intra-particle diffusion model (R2 = 0.96)	1115:1160	The Pb2+ adsorption process on MCP was a multistep diffusion-controlled phenomenon evidenced by the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96).
34628317	3	80	theme	straight-forward	460:475	arg1	approach					495:502	a straight-forward one pot synthesis approach	458:502	a straight-forward one pot synthesis approach to evaluate its lead (Pb2+) removal capacity from aqueous solution	458:569	In the present study, a magnetic chitosan-palygorskite (MCP) nanocomposite was prepared through a straight-forward one pot synthesis approach to evaluate its lead (Pb2+) removal capacity from aqueous solution.
34628317	7	81	theme	Pb2+	1231:1234	arg1	concentration					1236:1248	low Pb2+ concentration	1227:1248	low Pb2+ concentration	1227:1248	Thermodynamic analysis suggested that the adsorption process at low Pb2+ concentration was controlled by chemisorption, whereas that at high Pb2+ concentration was dominated by physical adsorption.
34628317	5	82	theme	58.5 mg	850:856	arg1	g-1					863:865	58.5 mg Pb2+ g-1	850:865	58.5 mg Pb2+ g-1 of MCP	850:872	The MCP nanocomposite was capable to remove up to 58.5 mg Pb2+ g-1 of MCP from water with a good agreement of experimental data to the Langmuir isotherm model (R2 = 0.98).
34628317	9	83	theme	82	1583:1584	arg1	%					1585:1585	%	1585:1585	%	1585:1585	During regeneration, the MCP retained 82% Pb2+ adsorption capacity following four adsorption-desorption cycles with ease to recover the adsorbent using its strong magnetic property.
34628317	9	84	theme	magnetic	1708:1715	arg1	property					1717:1724	its strong magnetic property	1697:1724	its strong magnetic property	1697:1724	During regeneration, the MCP retained 82% Pb2+ adsorption capacity following four adsorption-desorption cycles with ease to recover the adsorbent using its strong magnetic property.
34628317	6	85	theme	data	1107:1110	arg1	well-fitting					1072:1083	the well-fitting	1068:1083	the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96)	1068:1160	The Pb2+ adsorption process on MCP was a multistep diffusion-controlled phenomenon evidenced by the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96).
34628317	10	86	theme	easily-prepared	1794:1808	arg1	nanocomposite					1810:1822	the easily-prepared nanocomposite	1790:1822	the easily-prepared nanocomposite which holds outstanding potential to be used as an inexpensive and green adsorbent for remediating Pb2+ contaminated water	1790:1945	These findings highlight the enhanced structural properties of the easily-prepared nanocomposite which holds outstanding potential to be used as an inexpensive and green adsorbent for remediating Pb2+ contaminated water.
34628317	7	87	theme	adsorption	1205:1214	arg1	process					1216:1222	the adsorption process	1201:1222	the adsorption process at low Pb2+ concentration	1201:1248	Thermodynamic analysis suggested that the adsorption process at low Pb2+ concentration was controlled by chemisorption, whereas that at high Pb2+ concentration was dominated by physical adsorption.
34628317	8	88	from	adsorption	1455:1464	arg1	MCP					1469:1471	MCP	1469:1471	MCP	1469:1471	X-ray photoelectron and Fourier transform infrared spectroscopy results suggested that the Pb adsorption on MCP was governed by surface complexation and chemical reduction mechanisms.
34628317	7	89	theme	physical	1340:1347	arg1	adsorption					1349:1358	physical adsorption	1340:1358	physical adsorption	1340:1358	Thermodynamic analysis suggested that the adsorption process at low Pb2+ concentration was controlled by chemisorption, whereas that at high Pb2+ concentration was dominated by physical adsorption.
34628317	6	90	from	process	992:998	arg1	MCP					1003:1005	MCP	1003:1005	MCP	1003:1005	The Pb2+ adsorption process on MCP was a multistep diffusion-controlled phenomenon evidenced by the well-fitting of kinetic adsorption data to the intra-particle diffusion model (R2 = 0.96).
34628317	8	91	theme	chemical	1514:1521	arg1	mechanisms					1533:1542	chemical reduction mechanisms	1514:1542	chemical reduction mechanisms	1514:1542	X-ray photoelectron and Fourier transform infrared spectroscopy results suggested that the Pb adsorption on MCP was governed by surface complexation and chemical reduction mechanisms.
34628317	3	92	theme	lead	520:523	arg1	capacity					540:547	its lead (Pb2+) removal capacity	516:547	its lead (Pb2+) removal capacity from aqueous solution	516:569	In the present study, a magnetic chitosan-palygorskite (MCP) nanocomposite was prepared through a straight-forward one pot synthesis approach to evaluate its lead (Pb2+) removal capacity from aqueous solution.
34628317	1	93	theme	water	232:236	arg1	treatment					206:214	efficient treatment	196:214	efficient treatment of contaminated water	196:236	Development of polymeric magnetic adsorbents is a promising approach to obtain efficient treatment of contaminated water.
34655594	7	0	theme	UV	1175:1176	arg1	radiation					1178:1186	5 h. UV radiation	1170:1186	5 h. UV radiation	1170:1186	The results demonstrated that the highest dye removal was recorded at pH 10, with the equilibrium condition achieved within 5 h. UV radiation was shown to enhance dye removal.
34655594	6	1	theme	GGH	897:899	arg1	efficiency					866:875	The dye removal efficiency	850:875	The dye removal efficiency of the GGH and TiO2@GGH	850:899	The dye removal efficiency of the GGH and TiO2@GGH was evaluated in batch mode at ambient temperature under varying pH. The effect of UV radiation on the dye removal efficiency was also assessed.
34655594	4	2	from	presence	519:526	arg1	GGH					575:577	TiO2@GGH	570:577	TiO2@GGH	570:577	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH) was confirmed by scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis.
34655594	4	2	from	presence	519:526	arg1	matrix					562:567	the hydrogel matrix	549:567	the hydrogel matrix (TiO2@GGH)	549:578	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH) was confirmed by scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis.
34655594	5	3	theme	pore	830:833	arg1	sizes					835:839	pore sizes ~420 μm	830:847	pore sizes ~420 μm	830:847	The mercury intrusion and N2 sorption isotherm indicate the macroporous structure of the TiO2@GGH composite, showing the presence of pore sizes ~420 μm.
34655594	2	4	from	combination	314:324	arg1	hydrogel					296:303	composite hydrogel	286:303	composite hydrogel from the combination of guar gum (a neutral galactomannan polysaccharide) and TiO2	286:386	This work aims to synthesize composite hydrogel from the combination of guar gum (a neutral galactomannan polysaccharide) and TiO2.
34655594	4	5	theme	@	574:574	arg1	GGH					575:577	TiO2@GGH	570:577	TiO2@GGH	570:577	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH) was confirmed by scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis.
34655594	4	5	theme	@	574:574	arg1	matrix					562:567	the hydrogel matrix	549:567	the hydrogel matrix (TiO2@GGH)	549:578	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH) was confirmed by scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis.
34655594	0	6	theme	blue	82:85	arg1	methylene					72:80	methylene blue	72:85	methylene blue	72:85	TiO2/guar gum hydrogel composite for adsorption and photodegradation of methylene blue.
34655594	6	7	theme	@	896:896	arg1	GGH					897:899	the GGH and TiO2@GGH	880:899	GGH	897:899	The dye removal efficiency of the GGH and TiO2@GGH was evaluated in batch mode at ambient temperature under varying pH. The effect of UV radiation on the dye removal efficiency was also assessed.
34655594	4	8	theme	TiO2	570:573	arg1	GGH					575:577	TiO2@GGH	570:577	TiO2@GGH	570:577	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH) was confirmed by scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis.
34655594	4	8	theme	TiO2	570:573	arg1	matrix					562:567	the hydrogel matrix	549:567	the hydrogel matrix (TiO2@GGH)	549:578	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH) was confirmed by scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis.
34655594	6	9	theme	TiO2	892:895	arg1	GGH					897:899	the GGH and TiO2@GGH	880:899	GGH	897:899	The dye removal efficiency of the GGH and TiO2@GGH was evaluated in batch mode at ambient temperature under varying pH. The effect of UV radiation on the dye removal efficiency was also assessed.
34655594	3	10	theme	guar	407:410	arg1	gum					412:414	The TiO2-embedded guar gum hydrogel (TiO2@GGH)	389:434	The TiO2-embedded guar gum hydrogel (TiO2@GGH)	389:434	The TiO2-embedded guar gum hydrogel (TiO2@GGH) was utilized to remove methylene blue through adsorption and photodegradation.
34655594	8	11	theme	GGH	1290:1292	arg1	sorbent					1294:1300	GGH sorbent	1290:1300	GGH sorbent	1290:1300	The maximum adsorption capacity of TiO2@GGH is 198.61 mg g-1, while GGH sorbent is 188.53 mg g-1.
34655594	4	12	theme	X-ray	654:658	arg1	analysis					687:694	scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis	597:694	analysis	687:694	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH) was confirmed by scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis.
34655594	7	13	theme	5 h.	1170:1173	arg1	radiation					1178:1186	5 h. UV radiation	1170:1186	5 h. UV radiation	1170:1186	The results demonstrated that the highest dye removal was recorded at pH 10, with the equilibrium condition achieved within 5 h. UV radiation was shown to enhance dye removal.
34655594	8	14	theme	TiO2	1257:1260	arg1	GGH					1262:1264	TiO2@GGH	1257:1264	TiO2@GGH	1257:1264	The maximum adsorption capacity of TiO2@GGH is 198.61 mg g-1, while GGH sorbent is 188.53 mg g-1.
34655594	7	15	theme	highest	1080:1086	arg1	removal					1092:1098	the highest dye removal	1076:1098	the highest dye removal	1076:1098	The results demonstrated that the highest dye removal was recorded at pH 10, with the equilibrium condition achieved within 5 h. UV radiation was shown to enhance dye removal.
34655594	1	16	from	resources	149:157	arg1	development					92:102	The development	88:102	The development of porous adsorbent materials from renewable resources for water and wastewater treatment	88:192	The development of porous adsorbent materials from renewable resources for water and wastewater treatment has received considerable interest from academia and industry.
34655594	1	16	from	resources	149:157	arg1	materials					124:132	porous adsorbent materials	107:132	porous adsorbent materials from renewable resources for water and wastewater treatment	107:192	The development of porous adsorbent materials from renewable resources for water and wastewater treatment has received considerable interest from academia and industry.
34655594	6	17	from	temperature	940:950	arg1	mode					924:927	batch mode	918:927	batch mode at ambient temperature	918:950	The dye removal efficiency of the GGH and TiO2@GGH was evaluated in batch mode at ambient temperature under varying pH. The effect of UV radiation on the dye removal efficiency was also assessed.
34655594	4	18	attach	presence	519:526	arg1	GGH					575:577	TiO2@GGH	570:577	TiO2@GGH	570:577	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH) was confirmed by scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis.
34655594	4	18	attach	presence	519:526	arg1	matrix					562:567	the hydrogel matrix	549:567	the hydrogel matrix (TiO2@GGH)	549:578	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH) was confirmed by scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis.
34655594	4	18	attach	presence	519:526	arg2	particles					536:544	TiO2 particles	531:544	TiO2 particles	531:544	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH) was confirmed by scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis.
34655594	2	19	theme	galactomannan	349:361	arg1	polysaccharide					363:376	a neutral galactomannan polysaccharide	339:376	a neutral galactomannan polysaccharide	339:376	This work aims to synthesize composite hydrogel from the combination of guar gum (a neutral galactomannan polysaccharide) and TiO2.
34655594	2	19	theme	galactomannan	349:361	arg1	gum					334:336	guar gum	329:336	guar gum (a neutral galactomannan polysaccharide)	329:377	This work aims to synthesize composite hydrogel from the combination of guar gum (a neutral galactomannan polysaccharide) and TiO2.
34655594	3	20	dep	gum	412:414	arg1	hydrogel					416:423	hydrogel	416:423	The TiO2-embedded guar gum hydrogel (TiO2@GGH)	389:434	The TiO2-embedded guar gum hydrogel (TiO2@GGH) was utilized to remove methylene blue through adsorption and photodegradation.
34655594	3	20	dep	gum	412:414	arg1	GGH					431:433	TiO2@GGH	426:433	TiO2@GGH	426:433	The TiO2-embedded guar gum hydrogel (TiO2@GGH) was utilized to remove methylene blue through adsorption and photodegradation.
34655594	5	21	theme	sizes	835:839	arg1	presence					818:825	the presence	814:825	the presence of pore sizes ~420 μm	814:847	The mercury intrusion and N2 sorption isotherm indicate the macroporous structure of the TiO2@GGH composite, showing the presence of pore sizes ~420 μm.
34655594	4	22	theme	spectroscopy	674:685	arg1	analysis					687:694	scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis	597:694	analysis	687:694	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH) was confirmed by scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis.
34655594	1	23	theme	wastewater	173:182	arg1	treatment					184:192	wastewater treatment	173:192	wastewater treatment	173:192	The development of porous adsorbent materials from renewable resources for water and wastewater treatment has received considerable interest from academia and industry.
34655594	5	24	theme	macroporous	757:767	arg1	structure					769:777	the macroporous structure	753:777	the macroporous structure of the TiO2@GGH composite	753:803	The mercury intrusion and N2 sorption isotherm indicate the macroporous structure of the TiO2@GGH composite, showing the presence of pore sizes ~420 μm.
34655594	2	25	theme	composite	286:294	arg1	hydrogel					296:303	composite hydrogel	286:303	composite hydrogel from the combination of guar gum (a neutral galactomannan polysaccharide) and TiO2	286:386	This work aims to synthesize composite hydrogel from the combination of guar gum (a neutral galactomannan polysaccharide) and TiO2.
34655594	3	26	theme	TiO2-embedded	393:405	arg1	gum					412:414	The TiO2-embedded guar gum hydrogel (TiO2@GGH)	389:434	The TiO2-embedded guar gum hydrogel (TiO2@GGH)	389:434	The TiO2-embedded guar gum hydrogel (TiO2@GGH) was utilized to remove methylene blue through adsorption and photodegradation.
34655594	5	27	theme	TiO2	786:789	arg1	composite					795:803	the TiO2@GGH composite	782:803	the TiO2@GGH composite	782:803	The mercury intrusion and N2 sorption isotherm indicate the macroporous structure of the TiO2@GGH composite, showing the presence of pore sizes ~420 μm.
34655594	6	28	from	effect	974:979	arg1	efficiency					1016:1025	the dye removal efficiency	1000:1025	the dye removal efficiency	1000:1025	The dye removal efficiency of the GGH and TiO2@GGH was evaluated in batch mode at ambient temperature under varying pH. The effect of UV radiation on the dye removal efficiency was also assessed.
34655594	2	29	theme	gum	334:336	arg1	combination					314:324	the combination	310:324	the combination of guar gum (a neutral galactomannan polysaccharide) and TiO2	310:386	This work aims to synthesize composite hydrogel from the combination of guar gum (a neutral galactomannan polysaccharide) and TiO2.
34655594	6	30	theme	GGH	884:886	arg1	efficiency					866:875	The dye removal efficiency	850:875	The dye removal efficiency of the GGH and TiO2@GGH	850:899	The dye removal efficiency of the GGH and TiO2@GGH was evaluated in batch mode at ambient temperature under varying pH. The effect of UV radiation on the dye removal efficiency was also assessed.
34655594	3	31	theme	@	430:430	arg1	hydrogel					416:423	hydrogel	416:423	The TiO2-embedded guar gum hydrogel (TiO2@GGH)	389:434	The TiO2-embedded guar gum hydrogel (TiO2@GGH) was utilized to remove methylene blue through adsorption and photodegradation.
34655594	3	31	theme	@	430:430	arg1	GGH					431:433	TiO2@GGH	426:433	TiO2@GGH	426:433	The TiO2-embedded guar gum hydrogel (TiO2@GGH) was utilized to remove methylene blue through adsorption and photodegradation.
34655594	0	32	theme	TiO2/guar	0:8	arg1	gum					10:12	TiO2/guar gum	0:12	TiO2/guar gum	0:12	TiO2/guar gum hydrogel composite for adsorption and photodegradation of methylene blue.
34655594	4	33	theme	hydrogel	553:560	arg1	GGH					575:577	TiO2@GGH	570:577	TiO2@GGH	570:577	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH) was confirmed by scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis.
34655594	4	33	theme	hydrogel	553:560	arg1	matrix					562:567	the hydrogel matrix	549:567	the hydrogel matrix (TiO2@GGH)	549:578	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH) was confirmed by scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis.
34655594	2	34	theme	guar	329:332	arg1	polysaccharide					363:376	a neutral galactomannan polysaccharide	339:376	a neutral galactomannan polysaccharide	339:376	This work aims to synthesize composite hydrogel from the combination of guar gum (a neutral galactomannan polysaccharide) and TiO2.
34655594	2	34	theme	guar	329:332	arg1	gum					334:336	guar gum	329:336	guar gum (a neutral galactomannan polysaccharide)	329:377	This work aims to synthesize composite hydrogel from the combination of guar gum (a neutral galactomannan polysaccharide) and TiO2.
34655594	4	35	theme	photoelectron	660:672	arg1	analysis					687:694	scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis	597:694	analysis	687:694	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH) was confirmed by scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis.
34655594	7	36	theme	dye	1088:1090	arg1	removal					1092:1098	the highest dye removal	1076:1098	the highest dye removal	1076:1098	The results demonstrated that the highest dye removal was recorded at pH 10, with the equilibrium condition achieved within 5 h. UV radiation was shown to enhance dye removal.
34655594	7	37	theme	dye	1209:1211	arg1	removal					1213:1219	dye removal	1209:1219	dye removal	1209:1219	The results demonstrated that the highest dye removal was recorded at pH 10, with the equilibrium condition achieved within 5 h. UV radiation was shown to enhance dye removal.
34655594	1	38	theme	considerable	207:218	arg1	interest					220:227	considerable interest	207:227	considerable interest	207:227	The development of porous adsorbent materials from renewable resources for water and wastewater treatment has received considerable interest from academia and industry.
34655594	3	39	used	utilized	440:447	arg2	gum					412:414	The TiO2-embedded guar gum hydrogel (TiO2@GGH)	389:434	The TiO2-embedded guar gum hydrogel (TiO2@GGH)	389:434	The TiO2-embedded guar gum hydrogel (TiO2@GGH) was utilized to remove methylene blue through adsorption and photodegradation.
34655594	6	40	theme	ambient	932:938	arg1	temperature					940:950	ambient temperature	932:950	ambient temperature	932:950	The dye removal efficiency of the GGH and TiO2@GGH was evaluated in batch mode at ambient temperature under varying pH. The effect of UV radiation on the dye removal efficiency was also assessed.
34655594	1	41	theme	porous	107:112	arg1	materials					124:132	porous adsorbent materials	107:132	porous adsorbent materials from renewable resources for water and wastewater treatment	107:192	The development of porous adsorbent materials from renewable resources for water and wastewater treatment has received considerable interest from academia and industry.
34655594	4	42	theme	electron	606:613	arg1	X-ray					644:648	scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis	597:694	X-ray	644:648	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH) was confirmed by scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis.
34655594	5	43	theme	@	790:790	arg1	composite					795:803	the TiO2@GGH composite	782:803	the TiO2@GGH composite	782:803	The mercury intrusion and N2 sorption isotherm indicate the macroporous structure of the TiO2@GGH composite, showing the presence of pore sizes ~420 μm.
34655594	2	44	theme	neutral	341:347	arg1	polysaccharide					363:376	a neutral galactomannan polysaccharide	339:376	a neutral galactomannan polysaccharide	339:376	This work aims to synthesize composite hydrogel from the combination of guar gum (a neutral galactomannan polysaccharide) and TiO2.
34655594	2	44	theme	neutral	341:347	arg1	gum					334:336	guar gum	329:336	guar gum (a neutral galactomannan polysaccharide)	329:377	This work aims to synthesize composite hydrogel from the combination of guar gum (a neutral galactomannan polysaccharide) and TiO2.
34655594	5	45	theme	N2	723:724	arg1	isotherm					735:742	N2 sorption isotherm	723:742	N2 sorption isotherm	723:742	The mercury intrusion and N2 sorption isotherm indicate the macroporous structure of the TiO2@GGH composite, showing the presence of pore sizes ~420 μm.
34655594	6	46	theme	removal	858:864	arg1	efficiency					866:875	The dye removal efficiency	850:875	The dye removal efficiency of the GGH and TiO2@GGH	850:899	The dye removal efficiency of the GGH and TiO2@GGH was evaluated in batch mode at ambient temperature under varying pH. The effect of UV radiation on the dye removal efficiency was also assessed.
34655594	6	47	theme	dye	1004:1006	arg1	efficiency					1016:1025	the dye removal efficiency	1000:1025	the dye removal efficiency	1000:1025	The dye removal efficiency of the GGH and TiO2@GGH was evaluated in batch mode at ambient temperature under varying pH. The effect of UV radiation on the dye removal efficiency was also assessed.
34655594	3	48	theme	TiO2	426:429	arg1	hydrogel					416:423	hydrogel	416:423	The TiO2-embedded guar gum hydrogel (TiO2@GGH)	389:434	The TiO2-embedded guar gum hydrogel (TiO2@GGH) was utilized to remove methylene blue through adsorption and photodegradation.
34655594	3	48	theme	TiO2	426:429	arg1	GGH					431:433	TiO2@GGH	426:433	TiO2@GGH	426:433	The TiO2-embedded guar gum hydrogel (TiO2@GGH) was utilized to remove methylene blue through adsorption and photodegradation.
34655594	4	49	theme	TiO2	531:534	arg1	particles					536:544	TiO2 particles	531:544	TiO2 particles	531:544	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH) was confirmed by scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis.
34655594	5	50	theme	GGH	791:793	arg1	composite					795:803	the TiO2@GGH composite	782:803	the TiO2@GGH composite	782:803	The mercury intrusion and N2 sorption isotherm indicate the macroporous structure of the TiO2@GGH composite, showing the presence of pore sizes ~420 μm.
34655594	6	51	theme	dye	854:856	arg1	efficiency					866:875	The dye removal efficiency	850:875	The dye removal efficiency of the GGH and TiO2@GGH	850:899	The dye removal efficiency of the GGH and TiO2@GGH was evaluated in batch mode at ambient temperature under varying pH. The effect of UV radiation on the dye removal efficiency was also assessed.
34655594	6	52	theme	UV	984:985	arg1	radiation					987:995	UV radiation	984:995	UV radiation	984:995	The dye removal efficiency of the GGH and TiO2@GGH was evaluated in batch mode at ambient temperature under varying pH. The effect of UV radiation on the dye removal efficiency was also assessed.
34655594	6	53	theme	removal	1008:1014	arg1	efficiency					1016:1025	the dye removal efficiency	1000:1025	the dye removal efficiency	1000:1025	The dye removal efficiency of the GGH and TiO2@GGH was evaluated in batch mode at ambient temperature under varying pH. The effect of UV radiation on the dye removal efficiency was also assessed.
34655594	4	54	theme	dispersive	633:642	arg1	X-ray					644:648	scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis	597:694	X-ray	644:648	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH) was confirmed by scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis.
34655594	5	55	theme	composite	795:803	arg1	structure					769:777	the macroporous structure	753:777	the macroporous structure of the TiO2@GGH composite	753:803	The mercury intrusion and N2 sorption isotherm indicate the macroporous structure of the TiO2@GGH composite, showing the presence of pore sizes ~420 μm.
34655594	6	56	theme	batch	918:922	arg1	mode					924:927	batch mode	918:927	batch mode at ambient temperature	918:950	The dye removal efficiency of the GGH and TiO2@GGH was evaluated in batch mode at ambient temperature under varying pH. The effect of UV radiation on the dye removal efficiency was also assessed.
34655594	9	57	theme	photodegradation	1374:1389	arg1	effect					1391:1396	the photodegradation effect	1370:1396	the photodegradation effect	1370:1396	The results imply that UV radiation gives rise to the photodegradation effect.
34655594	1	58	theme	adsorbent	114:122	arg1	materials					124:132	porous adsorbent materials	107:132	porous adsorbent materials from renewable resources for water and wastewater treatment	107:192	The development of porous adsorbent materials from renewable resources for water and wastewater treatment has received considerable interest from academia and industry.
34655594	5	59	theme	sorption	726:733	arg1	isotherm					735:742	N2 sorption isotherm	723:742	N2 sorption isotherm	723:742	The mercury intrusion and N2 sorption isotherm indicate the macroporous structure of the TiO2@GGH composite, showing the presence of pore sizes ~420 μm.
34655594	1	60	theme	materials	124:132	arg1	development					92:102	The development	88:102	The development of porous adsorbent materials from renewable resources for water and wastewater treatment	88:192	The development of porous adsorbent materials from renewable resources for water and wastewater treatment has received considerable interest from academia and industry.
34655594	4	61	theme	particles	536:544	arg1	presence					519:526	The presence	515:526	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH)	515:578	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH) was confirmed by scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis.
34655594	8	62	theme	adsorption	1234:1243	arg1	capacity					1245:1252	The maximum adsorption capacity	1222:1252	The maximum adsorption capacity of TiO2@GGH	1222:1264	The maximum adsorption capacity of TiO2@GGH is 198.61 mg g-1, while GGH sorbent is 188.53 mg g-1.
34655594	8	62	theme	adsorption	1234:1243	arg1	198.61 mg g-1					1269:1281	198.61 mg g-1	1269:1281	198.61 mg g-1	1269:1281	The maximum adsorption capacity of TiO2@GGH is 198.61 mg g-1, while GGH sorbent is 188.53 mg g-1.
34655594	8	63	theme	@	1261:1261	arg1	GGH					1262:1264	TiO2@GGH	1257:1264	TiO2@GGH	1257:1264	The maximum adsorption capacity of TiO2@GGH is 198.61 mg g-1, while GGH sorbent is 188.53 mg g-1.
34655594	5	64	theme	mercury	701:707	arg1	intrusion					709:717	The mercury intrusion	697:717	The mercury intrusion	697:717	The mercury intrusion and N2 sorption isotherm indicate the macroporous structure of the TiO2@GGH composite, showing the presence of pore sizes ~420 μm.
34655594	0	65	dep	hydrogel	14:21	arg1	composite					23:31	composite	23:31	composite	23:31	TiO2/guar gum hydrogel composite for adsorption and photodegradation of methylene blue.
34655594	9	66	theme	UV	1343:1344	arg1	radiation					1346:1354	UV radiation	1343:1354	UV radiation	1343:1354	The results imply that UV radiation gives rise to the photodegradation effect.
34655594	1	67	theme	renewable	139:147	arg1	resources					149:157	renewable resources	139:157	renewable resources for water and wastewater treatment	139:192	The development of porous adsorbent materials from renewable resources for water and wastewater treatment has received considerable interest from academia and industry.
34655594	6	68	theme	radiation	987:995	arg1	effect					974:979	The effect	970:979	The effect of UV radiation on the dye removal efficiency	970:1025	The dye removal efficiency of the GGH and TiO2@GGH was evaluated in batch mode at ambient temperature under varying pH. The effect of UV radiation on the dye removal efficiency was also assessed.
34655594	0	69	theme	methylene	72:80	arg1	adsorption					37:46	adsorption	37:46	adsorption	37:46	TiO2/guar gum hydrogel composite for adsorption and photodegradation of methylene blue.
34655594	0	69	theme	methylene	72:80	arg1	photodegradation					52:67	photodegradation	52:67	photodegradation	52:67	TiO2/guar gum hydrogel composite for adsorption and photodegradation of methylene blue.
34655594	8	70	theme	maximum	1226:1232	arg1	capacity					1245:1252	The maximum adsorption capacity	1222:1252	The maximum adsorption capacity of TiO2@GGH	1222:1264	The maximum adsorption capacity of TiO2@GGH is 198.61 mg g-1, while GGH sorbent is 188.53 mg g-1.
34655594	8	70	theme	maximum	1226:1232	arg1	198.61 mg g-1					1269:1281	198.61 mg g-1	1269:1281	198.61 mg g-1	1269:1281	The maximum adsorption capacity of TiO2@GGH is 198.61 mg g-1, while GGH sorbent is 188.53 mg g-1.
34655594	4	71	theme	microscopy-energy	615:631	arg1	X-ray					644:648	scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis	597:694	X-ray	644:648	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH) was confirmed by scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis.
34655594	2	72	theme	TiO2	383:386	arg1	combination					314:324	the combination	310:324	the combination of guar gum (a neutral galactomannan polysaccharide) and TiO2	310:386	This work aims to synthesize composite hydrogel from the combination of guar gum (a neutral galactomannan polysaccharide) and TiO2.
34655594	1	73	from	development	92:102	arg1	resources					149:157	renewable resources	139:157	renewable resources for water and wastewater treatment	139:192	The development of porous adsorbent materials from renewable resources for water and wastewater treatment has received considerable interest from academia and industry.
34655594	4	74	theme	scanning	597:604	arg1	X-ray					644:648	scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis	597:694	X-ray	644:648	The presence of TiO2 particles in the hydrogel matrix (TiO2@GGH) was confirmed by scanning electron microscopy-energy dispersive X-ray and X-ray photoelectron spectroscopy analysis.
34655594	6	75	dep	pH.	966:968	arg1	assessed					1036:1043	assessed	1036:1043	was also assessed	1027:1043	The dye removal efficiency of the GGH and TiO2@GGH was evaluated in batch mode at ambient temperature under varying pH. The effect of UV radiation on the dye removal efficiency was also assessed.
34655594	8	76	theme	GGH	1262:1264	arg1	capacity					1245:1252	The maximum adsorption capacity	1222:1252	The maximum adsorption capacity of TiO2@GGH	1222:1264	The maximum adsorption capacity of TiO2@GGH is 198.61 mg g-1, while GGH sorbent is 188.53 mg g-1.
34655594	8	76	theme	GGH	1262:1264	arg1	198.61 mg g-1					1269:1281	198.61 mg g-1	1269:1281	198.61 mg g-1	1269:1281	The maximum adsorption capacity of TiO2@GGH is 198.61 mg g-1, while GGH sorbent is 188.53 mg g-1.
34655594	7	77	theme	equilibrium	1132:1142	arg1	condition					1144:1152	the equilibrium condition	1128:1152	the equilibrium condition achieved within 5 h. UV radiation	1128:1186	The results demonstrated that the highest dye removal was recorded at pH 10, with the equilibrium condition achieved within 5 h. UV radiation was shown to enhance dye removal.
32578166	6	0	theme	angina	898:903	arg1	risk					842:845	30-day risk	835:845	30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs	835:1021	Outcomes included 30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs.
32578166	11	1	theme	NSTEMI	1874:1879	arg1	patients					1881:1888	NSTEMI patients	1874:1888	NSTEMI patients	1874:1888	CONCLUSIONS Enoxaparin was associated with a lower risk of ischemic complications (including death), lower costs, and better safety than UFH among NSTEMI patients.
32578166	1	2	theme	unstable	296:303	arg1	UA					313:314	UA	313:314	UA	313:314	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	1	2	theme	unstable	296:303	arg1	angina					305:310	unstable angina	296:310	unstable angina (UA)	296:315	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	10	3	theme	Cost	1577:1580	arg1	savings					1582:1588	Cost savings	1577:1588	Cost savings per patient during index admission and 30-day follow-up for enoxaparin over UFH	1577:1668	Cost savings per patient during index admission and 30-day follow-up for enoxaparin over UFH was $2972 for UA, $2475 for NSTEMI, and $3050 for STEMI.
32578166	7	4	used	used	1053:1056	arg2	regression					1038:1047	Multivariable regression	1024:1047	Multivariable regression	1024:1047	Multivariable regression was used to compare outcomes between enoxaparin and UFH monotherapy.
32578166	6	5	theme	infarction 	871:881	arg1	risk					842:845	30-day risk	835:845	30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs	835:1021	Outcomes included 30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs.
32578166	12	6	theme	anticoagulants	1923:1936	arg1	selection					1910:1918	Improving upstream selection	1891:1918	Improving upstream selection of anticoagulants in appropriate populations	1891:1963	Improving upstream selection of anticoagulants in appropriate populations may help optimize clinical outcomes and costs.
32578166	11	7	theme	lower	1828:1832	arg1	costs					1834:1838	lower costs	1828:1838	lower costs	1828:1838	CONCLUSIONS Enoxaparin was associated with a lower risk of ischemic complications (including death), lower costs, and better safety than UFH among NSTEMI patients.
32578166	8	8	theme	enoxaparin	1226:1235	arg1	monotherapy					1237:1247	enoxaparin monotherapy	1226:1247	enoxaparin monotherapy	1226:1247	RESULTS Among 1,048,053 eligible patients (UA: 219,259; NSTEMI: 582,134; STEMI: 246,660), the prevalence of enoxaparin monotherapy was 12.0%, 13.9%, and 5.1%, and the prevalence of UFH monotherapy was 45.1%, 43.1% and 59.8%, for UA, NSTEMI, and STEMI patients, respectively.
32578166	5	9	theme	inpatients	703:712	arg1	hospitals					692:700	859 US hospitals	685:700	859 US hospitals	685:700	METHODS Using data from 859 US hospitals, inpatients ≥ 18 years of age with a diagnosis of an initial episode of ACS between 2010 and 2016 were identified.
32578166	5	9	theme	inpatients	703:712	arg1	≥ 18 years					714:723	inpatients ≥ 18 years	703:723	inpatients ≥ 18 years of age with a diagnosis of an initial episode of ACS between 2010 and 2016	703:798	METHODS Using data from 859 US hospitals, inpatients ≥ 18 years of age with a diagnosis of an initial episode of ACS between 2010 and 2016 were identified.
32578166	6	10	theme	ischemic	939:946	arg1	complication					948:959	composite ischemic complication	929:959	composite ischemic complication (having MI/recurrent angina/death)	929:994	Outcomes included 30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs.
32578166	12	11	theme	Improving	1891:1899	arg1	selection					1910:1918	Improving upstream selection	1891:1918	Improving upstream selection of anticoagulants in appropriate populations	1891:1963	Improving upstream selection of anticoagulants in appropriate populations may help optimize clinical outcomes and costs.
32578166	1	12	theme	acute	255:259	arg1	ACS					280:282	ACS	280:282	ACS	280:282	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	1	12	theme	acute	255:259	arg1	syndrome					270:277	acute coronary syndrome	255:277	acute coronary syndrome (ACS)	255:283	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	10	13	theme	30-day	1629:1634	arg1	follow-up					1636:1644	30-day follow-up	1629:1644	30-day follow-up for enoxaparin over UFH	1629:1668	Cost savings per patient during index admission and 30-day follow-up for enoxaparin over UFH was $2972 for UA, $2475 for NSTEMI, and $3050 for STEMI.
32578166	11	14	dep	complications	1795:1807	arg1	including					1810:1818	including	1810:1818	including death	1810:1824	CONCLUSIONS Enoxaparin was associated with a lower risk of ischemic complications (including death), lower costs, and better safety than UFH among NSTEMI patients.
32578166	6	15	theme	in-hospital	906:916	arg1	mortality					918:926	in-hospital mortality	906:926	in-hospital mortality	906:926	Outcomes included 30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs.
32578166	4	16	dep	enoxaparin	607:616	arg1	monotherapy					626:636	monotherapy	626:636	monotherapy	626:636	This study examined the differences in utilization, effectiveness, safety, and costs in treating ACS between enoxaparin and UFH monotherapy using real-world data.
32578166	0	17	theme	Unfractionated	69:82	arg1	Heparin					84:90	Unfractionated Heparin	69:90	Unfractionated Heparin	69:90	Comparative Effectiveness and Costs of Enoxaparin Monotherapy Versus Unfractionated Heparin Monotherapy in Treating Acute Coronary Syndrome.
32578166	11	18	theme	lower	1772:1776	arg1	risk					1778:1781	a lower risk	1770:1781	a lower risk of ischemic complications (including death)	1770:1825	CONCLUSIONS Enoxaparin was associated with a lower risk of ischemic complications (including death), lower costs, and better safety than UFH among NSTEMI patients.
32578166	8	19	theme	STEMI	1363:1367	arg1	patients					1369:1376	STEMI patients	1363:1376	STEMI patients	1363:1376	RESULTS Among 1,048,053 eligible patients (UA: 219,259; NSTEMI: 582,134; STEMI: 246,660), the prevalence of enoxaparin monotherapy was 12.0%, 13.9%, and 5.1%, and the prevalence of UFH monotherapy was 45.1%, 43.1% and 59.8%, for UA, NSTEMI, and STEMI patients, respectively.
32578166	5	20	theme	episode	763:769	arg1	diagnosis					739:747	a diagnosis	737:747	a diagnosis of an initial episode of ACS between 2010 and 2016	737:798	METHODS Using data from 859 US hospitals, inpatients ≥ 18 years of age with a diagnosis of an initial episode of ACS between 2010 and 2016 were identified.
32578166	9	21	from	bleeding	1551:1558	arg1	patients					1567:1574	all patients	1563:1574	all patients	1563:1574	Enoxaparin was associated with a lower risk of ischemic complications and death among NSTEMI, but not in UA or STEMI patients, and with a lower risk of major bleeding in all patients.
32578166	8	22	dep	UA	1161:1162	arg1	582,134					1182:1188	582,134	1182:1188	582,134	1182:1188	RESULTS Among 1,048,053 eligible patients (UA: 219,259; NSTEMI: 582,134; STEMI: 246,660), the prevalence of enoxaparin monotherapy was 12.0%, 13.9%, and 5.1%, and the prevalence of UFH monotherapy was 45.1%, 43.1% and 59.8%, for UA, NSTEMI, and STEMI patients, respectively.
32578166	8	22	dep	UA	1161:1162	arg1	STEMI					1191:1195	STEMI	1191:1195	UA: 219,259; NSTEMI: 582,134; STEMI: 246,660	1161:1204	RESULTS Among 1,048,053 eligible patients (UA: 219,259; NSTEMI: 582,134; STEMI: 246,660), the prevalence of enoxaparin monotherapy was 12.0%, 13.9%, and 5.1%, and the prevalence of UFH monotherapy was 45.1%, 43.1% and 59.8%, for UA, NSTEMI, and STEMI patients, respectively.
32578166	8	22	dep	UA	1161:1162	arg1	219,259					1165:1171	219,259	1165:1171	219,259	1165:1171	RESULTS Among 1,048,053 eligible patients (UA: 219,259; NSTEMI: 582,134; STEMI: 246,660), the prevalence of enoxaparin monotherapy was 12.0%, 13.9%, and 5.1%, and the prevalence of UFH monotherapy was 45.1%, 43.1% and 59.8%, for UA, NSTEMI, and STEMI patients, respectively.
32578166	8	22	dep	UA	1161:1162	arg1	NSTEMI					1174:1179	NSTEMI	1174:1179	UA: 219,259; NSTEMI: 582,134; STEMI: 246,660	1161:1204	RESULTS Among 1,048,053 eligible patients (UA: 219,259; NSTEMI: 582,134; STEMI: 246,660), the prevalence of enoxaparin monotherapy was 12.0%, 13.9%, and 5.1%, and the prevalence of UFH monotherapy was 45.1%, 43.1% and 59.8%, for UA, NSTEMI, and STEMI patients, respectively.
32578166	8	22	dep	UA	1161:1162	arg1	246,660					1198:1204	246,660	1198:1204	246,660	1198:1204	RESULTS Among 1,048,053 eligible patients (UA: 219,259; NSTEMI: 582,134; STEMI: 246,660), the prevalence of enoxaparin monotherapy was 12.0%, 13.9%, and 5.1%, and the prevalence of UFH monotherapy was 45.1%, 43.1% and 59.8%, for UA, NSTEMI, and STEMI patients, respectively.
32578166	12	23	from	selection	1910:1918	arg1	populations					1953:1963	appropriate populations	1941:1963	appropriate populations	1941:1963	Improving upstream selection of anticoagulants in appropriate populations may help optimize clinical outcomes and costs.
32578166	6	24	theme	MI/recurrent	969:980	arg1	angina/death					982:993	MI/recurrent angina/death	969:993	MI/recurrent angina/death	969:993	Outcomes included 30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs.
32578166	8	25	dep	patients	1151:1158	arg1	UA					1161:1162	UA	1161:1162	UA: 219,259; NSTEMI: 582,134; STEMI: 246,660	1161:1204	RESULTS Among 1,048,053 eligible patients (UA: 219,259; NSTEMI: 582,134; STEMI: 246,660), the prevalence of enoxaparin monotherapy was 12.0%, 13.9%, and 5.1%, and the prevalence of UFH monotherapy was 45.1%, 43.1% and 59.8%, for UA, NSTEMI, and STEMI patients, respectively.
32578166	11	26	theme	CONCLUSIONS	1727:1737	arg1	Enoxaparin					1739:1748	CONCLUSIONS Enoxaparin	1727:1748	CONCLUSIONS Enoxaparin	1727:1748	CONCLUSIONS Enoxaparin was associated with a lower risk of ischemic complications (including death), lower costs, and better safety than UFH among NSTEMI patients.
32578166	2	27	theme	Prior	398:402	arg1	evidence					424:431	Prior efficacy and safety evidence	398:431	Prior efficacy and safety evidence	398:431	Prior efficacy and safety evidence are mainly from clinical trials.
32578166	4	28	from	costs	577:581	arg1	ACS					595:597	treating ACS	586:597	treating ACS	586:597	This study examined the differences in utilization, effectiveness, safety, and costs in treating ACS between enoxaparin and UFH monotherapy using real-world data.
32578166	0	29	theme	Enoxaparin	39:48	arg1	Monotherapy					50:60	Enoxaparin Monotherapy	39:60	Enoxaparin Monotherapy	39:60	Comparative Effectiveness and Costs of Enoxaparin Monotherapy Versus Unfractionated Heparin Monotherapy in Treating Acute Coronary Syndrome.
32578166	2	30	theme	clinical	449:456	arg1	trials					458:463	clinical trials	449:463	clinical trials	449:463	Prior efficacy and safety evidence are mainly from clinical trials.
32578166	0	31	theme	Comparative	0:10	arg1	Effectiveness					12:24	Comparative Effectiveness	0:24	Comparative Effectiveness	0:24	Comparative Effectiveness and Costs of Enoxaparin Monotherapy Versus Unfractionated Heparin Monotherapy in Treating Acute Coronary Syndrome.
32578166	9	32	theme	ischemic	1440:1447	arg1	complications					1449:1461	ischemic complications	1440:1461	ischemic complications	1440:1461	Enoxaparin was associated with a lower risk of ischemic complications and death among NSTEMI, but not in UA or STEMI patients, and with a lower risk of major bleeding in all patients.
32578166	8	33	theme	monotherapy	1303:1313	arg1	%					1323:1323	45.1%	1319:1323	45.1%	1319:1323	RESULTS Among 1,048,053 eligible patients (UA: 219,259; NSTEMI: 582,134; STEMI: 246,660), the prevalence of enoxaparin monotherapy was 12.0%, 13.9%, and 5.1%, and the prevalence of UFH monotherapy was 45.1%, 43.1% and 59.8%, for UA, NSTEMI, and STEMI patients, respectively.
32578166	8	33	theme	monotherapy	1303:1313	arg1	prevalence					1285:1294	the prevalence	1281:1294	the prevalence of UFH monotherapy	1281:1313	RESULTS Among 1,048,053 eligible patients (UA: 219,259; NSTEMI: 582,134; STEMI: 246,660), the prevalence of enoxaparin monotherapy was 12.0%, 13.9%, and 5.1%, and the prevalence of UFH monotherapy was 45.1%, 43.1% and 59.8%, for UA, NSTEMI, and STEMI patients, respectively.
32578166	3	34	theme	Economic	466:473	arg1	data					475:478	Economic data	466:478	Economic data	466:478	Economic data are insufficient.
32578166	6	35	theme	myocardial	860:869	arg1	MI					883:884	MI	883:884	MI	883:884	Outcomes included 30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs.
32578166	6	35	theme	myocardial	860:869	arg1	infarction 					871:881	non-fatal myocardial infarction 	850:881	non-fatal myocardial infarction (MI)	850:885	Outcomes included 30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs.
32578166	5	36	theme	ACS	774:776	arg1	episode					763:769	an initial episode	752:769	an initial episode of ACS between 2010 and 2016	752:798	METHODS Using data from 859 US hospitals, inpatients ≥ 18 years of age with a diagnosis of an initial episode of ACS between 2010 and 2016 were identified.
32578166	1	37	with	patients	241:248	arg1	ACS					280:282	ACS	280:282	ACS	280:282	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	1	37	with	patients	241:248	arg1	syndrome					270:277	acute coronary syndrome	255:277	acute coronary syndrome (ACS)	255:283	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	6	38	theme	costs	1017:1021	arg1	risk					842:845	30-day risk	835:845	30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs	835:1021	Outcomes included 30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs.
32578166	0	39	theme	Monotherapy	50:60	arg1	Costs					30:34	Costs	30:34	Costs	30:34	Comparative Effectiveness and Costs of Enoxaparin Monotherapy Versus Unfractionated Heparin Monotherapy in Treating Acute Coronary Syndrome.
32578166	0	39	theme	Monotherapy	50:60	arg1	Effectiveness					12:24	Comparative Effectiveness	0:24	Comparative Effectiveness	0:24	Comparative Effectiveness and Costs of Enoxaparin Monotherapy Versus Unfractionated Heparin Monotherapy in Treating Acute Coronary Syndrome.
32578166	6	40	theme	30-day	835:840	arg1	risk					842:845	30-day risk	835:845	30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs	835:1021	Outcomes included 30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs.
32578166	12	41	theme	appropriate	1941:1951	arg1	populations					1953:1963	appropriate populations	1941:1963	appropriate populations	1941:1963	Improving upstream selection of anticoagulants in appropriate populations may help optimize clinical outcomes and costs.
32578166	1	42	theme	coronary	261:268	arg1	ACS					280:282	ACS	280:282	ACS	280:282	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	1	42	theme	coronary	261:268	arg1	syndrome					270:277	acute coronary syndrome	255:277	acute coronary syndrome (ACS)	255:283	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	11	43	theme	complications	1795:1807	arg1	costs					1834:1838	lower costs	1828:1838	lower costs	1828:1838	CONCLUSIONS Enoxaparin was associated with a lower risk of ischemic complications (including death), lower costs, and better safety than UFH among NSTEMI patients.
32578166	11	43	theme	complications	1795:1807	arg1	risk					1778:1781	a lower risk	1770:1781	a lower risk of ischemic complications (including death)	1770:1825	CONCLUSIONS Enoxaparin was associated with a lower risk of ischemic complications (including death), lower costs, and better safety than UFH among NSTEMI patients.
32578166	11	43	theme	complications	1795:1807	arg1	safety					1852:1857	better safety	1845:1857	better safety	1845:1857	CONCLUSIONS Enoxaparin was associated with a lower risk of ischemic complications (including death), lower costs, and better safety than UFH among NSTEMI patients.
32578166	2	44	theme	efficacy	404:411	arg1	evidence					424:431	Prior efficacy and safety evidence	398:431	Prior efficacy and safety evidence	398:431	Prior efficacy and safety evidence are mainly from clinical trials.
32578166	4	45	from	differences	522:532	arg1	safety					565:570	safety	565:570	safety	565:570	This study examined the differences in utilization, effectiveness, safety, and costs in treating ACS between enoxaparin and UFH monotherapy using real-world data.
32578166	4	45	from	differences	522:532	arg1	costs					577:581	costs	577:581	costs	577:581	This study examined the differences in utilization, effectiveness, safety, and costs in treating ACS between enoxaparin and UFH monotherapy using real-world data.
32578166	4	45	from	differences	522:532	arg1	effectiveness					550:562	effectiveness	550:562	effectiveness	550:562	This study examined the differences in utilization, effectiveness, safety, and costs in treating ACS between enoxaparin and UFH monotherapy using real-world data.
32578166	4	45	from	differences	522:532	arg1	utilization					537:547	utilization	537:547	utilization	537:547	This study examined the differences in utilization, effectiveness, safety, and costs in treating ACS between enoxaparin and UFH monotherapy using real-world data.
32578166	8	46	theme	eligible	1142:1149	arg1	patients					1151:1158	1,048,053 eligible patients	1132:1158	1,048,053 eligible patients (UA: 219,259; NSTEMI: 582,134; STEMI: 246,660)	1132:1205	RESULTS Among 1,048,053 eligible patients (UA: 219,259; NSTEMI: 582,134; STEMI: 246,660), the prevalence of enoxaparin monotherapy was 12.0%, 13.9%, and 5.1%, and the prevalence of UFH monotherapy was 45.1%, 43.1% and 59.8%, for UA, NSTEMI, and STEMI patients, respectively.
32578166	8	46	theme	eligible	1142:1149	arg1	prevalence					1212:1221	the prevalence	1208:1221	the prevalence of enoxaparin monotherapy	1208:1247	RESULTS Among 1,048,053 eligible patients (UA: 219,259; NSTEMI: 582,134; STEMI: 246,660), the prevalence of enoxaparin monotherapy was 12.0%, 13.9%, and 5.1%, and the prevalence of UFH monotherapy was 45.1%, 43.1% and 59.8%, for UA, NSTEMI, and STEMI patients, respectively.
32578166	6	47	theme	recurrent	888:896	arg1	angina					898:903	recurrent angina	888:903	recurrent angina	888:903	Outcomes included 30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs.
32578166	9	48	theme	STEMI	1504:1508	arg1	patients					1510:1517	STEMI patients	1504:1517	STEMI patients	1504:1517	Enoxaparin was associated with a lower risk of ischemic complications and death among NSTEMI, but not in UA or STEMI patients, and with a lower risk of major bleeding in all patients.
32578166	4	49	theme	treating	586:593	arg1	ACS					595:597	treating ACS	586:597	treating ACS	586:597	This study examined the differences in utilization, effectiveness, safety, and costs in treating ACS between enoxaparin and UFH monotherapy using real-world data.
32578166	11	50	theme	ischemic	1786:1793	arg1	complications					1795:1807	ischemic complications	1786:1807	ischemic complications (including death)	1786:1825	CONCLUSIONS Enoxaparin was associated with a lower risk of ischemic complications (including death), lower costs, and better safety than UFH among NSTEMI patients.
32578166	12	51	theme	upstream	1901:1908	arg1	selection					1910:1918	Improving upstream selection	1891:1918	Improving upstream selection of anticoagulants in appropriate populations	1891:1963	Improving upstream selection of anticoagulants in appropriate populations may help optimize clinical outcomes and costs.
32578166	1	52	theme	guideline-recommended	200:220	arg1	Enoxaparin					152:161	BACKGROUND Enoxaparin	141:161	BACKGROUND Enoxaparin	141:161	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	1	52	theme	guideline-recommended	200:220	arg1	heparin					182:188	unfractionated heparin	167:188	unfractionated heparin (UFH)	167:194	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	1	52	theme	guideline-recommended	200:220	arg1	anticoagulants					222:235	guideline-recommended anticoagulants	200:235	guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI)	200:395	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	9	53	theme	lower	1531:1535	arg1	risk					1537:1540	a lower risk	1529:1540	a lower risk of major bleeding in all patients	1529:1574	Enoxaparin was associated with a lower risk of ischemic complications and death among NSTEMI, but not in UA or STEMI patients, and with a lower risk of major bleeding in all patients.
32578166	6	54	contain	having	962:967	arg1	complication					948:959	composite ischemic complication	929:959	composite ischemic complication (having MI/recurrent angina/death)	929:994	Outcomes included 30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs.
32578166	6	54	contain	having	962:967	arg2	angina/death					982:993	MI/recurrent angina/death	969:993	MI/recurrent angina/death	969:993	Outcomes included 30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs.
32578166	10	55	theme	index	1609:1613	arg1	admission					1615:1623	index admission	1609:1623	index admission	1609:1623	Cost savings per patient during index admission and 30-day follow-up for enoxaparin over UFH was $2972 for UA, $2475 for NSTEMI, and $3050 for STEMI.
32578166	6	56	theme	complication	948:959	arg1	risk					842:845	30-day risk	835:845	30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs	835:1021	Outcomes included 30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs.
32578166	11	57	theme	better	1845:1850	arg1	safety					1852:1857	better safety	1845:1857	better safety	1845:1857	CONCLUSIONS Enoxaparin was associated with a lower risk of ischemic complications (including death), lower costs, and better safety than UFH among NSTEMI patients.
32578166	6	58	theme	composite	929:937	arg1	complication					948:959	composite ischemic complication	929:959	composite ischemic complication (having MI/recurrent angina/death)	929:994	Outcomes included 30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs.
32578166	9	59	theme	bleeding	1551:1558	arg1	risk					1537:1540	a lower risk	1529:1540	a lower risk of major bleeding in all patients	1529:1574	Enoxaparin was associated with a lower risk of ischemic complications and death among NSTEMI, but not in UA or STEMI patients, and with a lower risk of major bleeding in all patients.
32578166	8	60	theme	monotherapy	1237:1247	arg1	patients					1151:1158	1,048,053 eligible patients	1132:1158	1,048,053 eligible patients (UA: 219,259; NSTEMI: 582,134; STEMI: 246,660)	1132:1205	RESULTS Among 1,048,053 eligible patients (UA: 219,259; NSTEMI: 582,134; STEMI: 246,660), the prevalence of enoxaparin monotherapy was 12.0%, 13.9%, and 5.1%, and the prevalence of UFH monotherapy was 45.1%, 43.1% and 59.8%, for UA, NSTEMI, and STEMI patients, respectively.
32578166	8	60	theme	monotherapy	1237:1247	arg1	prevalence					1212:1221	the prevalence	1208:1221	the prevalence of enoxaparin monotherapy	1208:1247	RESULTS Among 1,048,053 eligible patients (UA: 219,259; NSTEMI: 582,134; STEMI: 246,660), the prevalence of enoxaparin monotherapy was 12.0%, 13.9%, and 5.1%, and the prevalence of UFH monotherapy was 45.1%, 43.1% and 59.8%, for UA, NSTEMI, and STEMI patients, respectively.
32578166	7	61	dep	enoxaparin	1086:1095	arg1	monotherapy					1105:1115	monotherapy	1105:1115	monotherapy	1105:1115	Multivariable regression was used to compare outcomes between enoxaparin and UFH monotherapy.
32578166	5	62	theme	age	728:730	arg1	hospitals					692:700	859 US hospitals	685:700	859 US hospitals	685:700	METHODS Using data from 859 US hospitals, inpatients ≥ 18 years of age with a diagnosis of an initial episode of ACS between 2010 and 2016 were identified.
32578166	5	62	theme	age	728:730	arg1	≥ 18 years					714:723	inpatients ≥ 18 years	703:723	inpatients ≥ 18 years of age with a diagnosis of an initial episode of ACS between 2010 and 2016	703:798	METHODS Using data from 859 US hospitals, inpatients ≥ 18 years of age with a diagnosis of an initial episode of ACS between 2010 and 2016 were identified.
32578166	7	63	theme	Multivariable	1024:1036	arg1	regression					1038:1047	Multivariable regression	1024:1047	Multivariable regression	1024:1047	Multivariable regression was used to compare outcomes between enoxaparin and UFH monotherapy.
32578166	6	64	theme	mortality	918:926	arg1	risk					842:845	30-day risk	835:845	30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs	835:1021	Outcomes included 30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs.
32578166	5	65	theme	US	689:690	arg1	hospitals					692:700	859 US hospitals	685:700	859 US hospitals	685:700	METHODS Using data from 859 US hospitals, inpatients ≥ 18 years of age with a diagnosis of an initial episode of ACS between 2010 and 2016 were identified.
32578166	5	65	theme	US	689:690	arg1	≥ 18 years					714:723	inpatients ≥ 18 years	703:723	inpatients ≥ 18 years of age with a diagnosis of an initial episode of ACS between 2010 and 2016	703:798	METHODS Using data from 859 US hospitals, inpatients ≥ 18 years of age with a diagnosis of an initial episode of ACS between 2010 and 2016 were identified.
32578166	9	66	from	patients	1567:1574	arg1	risk					1537:1540	a lower risk	1529:1540	a lower risk of major bleeding in all patients	1529:1574	Enoxaparin was associated with a lower risk of ischemic complications and death among NSTEMI, but not in UA or STEMI patients, and with a lower risk of major bleeding in all patients.
32578166	9	66	from	patients	1567:1574	arg1	bleeding					1551:1558	bleeding	1551:1558	bleeding	1551:1558	Enoxaparin was associated with a lower risk of ischemic complications and death among NSTEMI, but not in UA or STEMI patients, and with a lower risk of major bleeding in all patients.
32578166	1	67	theme	unfractionated	167:180	arg1	Enoxaparin					152:161	BACKGROUND Enoxaparin	141:161	BACKGROUND Enoxaparin	141:161	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	1	67	theme	unfractionated	167:180	arg1	heparin					182:188	unfractionated heparin	167:188	unfractionated heparin (UFH)	167:194	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	1	67	theme	unfractionated	167:180	arg1	anticoagulants					222:235	guideline-recommended anticoagulants	200:235	guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI)	200:395	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	1	67	theme	unfractionated	167:180	arg1	UFH					191:193	UFH	191:193	UFH	191:193	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	1	68	theme	BACKGROUND	141:150	arg1	Enoxaparin					152:161	BACKGROUND Enoxaparin	141:161	BACKGROUND Enoxaparin	141:161	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	1	68	theme	BACKGROUND	141:150	arg1	heparin					182:188	unfractionated heparin	167:188	unfractionated heparin (UFH)	167:194	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	1	68	theme	BACKGROUND	141:150	arg1	anticoagulants					222:235	guideline-recommended anticoagulants	200:235	guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI)	200:395	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	6	69	theme	bleeding	1003:1010	arg1	risk					842:845	30-day risk	835:845	30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs	835:1021	Outcomes included 30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs.
32578166	5	70	theme	initial	755:761	arg1	episode					763:769	an initial episode	752:769	an initial episode of ACS between 2010 and 2016	752:798	METHODS Using data from 859 US hospitals, inpatients ≥ 18 years of age with a diagnosis of an initial episode of ACS between 2010 and 2016 were identified.
32578166	0	71	theme	Acute	116:120	arg1	Syndrome					131:138	Acute Coronary Syndrome	116:138	Acute Coronary Syndrome	116:138	Comparative Effectiveness and Costs of Enoxaparin Monotherapy Versus Unfractionated Heparin Monotherapy in Treating Acute Coronary Syndrome.
32578166	9	72	from	risk	1537:1540	arg1	patients					1567:1574	all patients	1563:1574	all patients	1563:1574	Enoxaparin was associated with a lower risk of ischemic complications and death among NSTEMI, but not in UA or STEMI patients, and with a lower risk of major bleeding in all patients.
32578166	1	73	theme	ST-segment	367:376	arg1	NSTEMI					389:394	NSTEMI	389:394	NSTEMI	389:394	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	1	73	theme	ST-segment	367:376	arg1	elevation					378:386	ST-segment elevation	367:386	ST-segment elevation (NSTEMI)	367:395	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	9	74	theme	lower	1426:1430	arg1	risk					1432:1435	a lower risk	1424:1435	a lower risk of ischemic complications	1424:1461	Enoxaparin was associated with a lower risk of ischemic complications and death among NSTEMI, but not in UA or STEMI patients, and with a lower risk of major bleeding in all patients.
32578166	4	75	from	safety	565:570	arg1	ACS					595:597	treating ACS	586:597	treating ACS	586:597	This study examined the differences in utilization, effectiveness, safety, and costs in treating ACS between enoxaparin and UFH monotherapy using real-world data.
32578166	9	76	theme	complications	1449:1461	arg1	risk					1432:1435	a lower risk	1424:1435	a lower risk of ischemic complications	1424:1461	Enoxaparin was associated with a lower risk of ischemic complications and death among NSTEMI, but not in UA or STEMI patients, and with a lower risk of major bleeding in all patients.
32578166	5	77	with	≥ 18 years	714:723	arg1	diagnosis					739:747	a diagnosis	737:747	a diagnosis of an initial episode of ACS between 2010 and 2016	737:798	METHODS Using data from 859 US hospitals, inpatients ≥ 18 years of age with a diagnosis of an initial episode of ACS between 2010 and 2016 were identified.
32578166	8	78	theme	UFH	1299:1301	arg1	monotherapy					1303:1313	UFH monotherapy	1299:1313	UFH monotherapy	1299:1313	RESULTS Among 1,048,053 eligible patients (UA: 219,259; NSTEMI: 582,134; STEMI: 246,660), the prevalence of enoxaparin monotherapy was 12.0%, 13.9%, and 5.1%, and the prevalence of UFH monotherapy was 45.1%, 43.1% and 59.8%, for UA, NSTEMI, and STEMI patients, respectively.
32578166	2	79	theme	safety	417:422	arg1	evidence					424:431	Prior efficacy and safety evidence	398:431	Prior efficacy and safety evidence	398:431	Prior efficacy and safety evidence are mainly from clinical trials.
32578166	4	80	from	effectiveness	550:562	arg1	ACS					595:597	treating ACS	586:597	treating ACS	586:597	This study examined the differences in utilization, effectiveness, safety, and costs in treating ACS between enoxaparin and UFH monotherapy using real-world data.
32578166	6	81	theme	non-fatal	850:858	arg1	MI					883:884	MI	883:884	MI	883:884	Outcomes included 30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs.
32578166	6	81	theme	non-fatal	850:858	arg1	infarction 					871:881	non-fatal myocardial infarction 	850:881	non-fatal myocardial infarction (MI)	850:885	Outcomes included 30-day risk of non-fatal myocardial infarction (MI), recurrent angina, in-hospital mortality, composite ischemic complication (having MI/recurrent angina/death), major bleeding, and costs.
32578166	0	82	theme	Coronary	122:129	arg1	Syndrome					131:138	Acute Coronary Syndrome	116:138	Acute Coronary Syndrome	116:138	Comparative Effectiveness and Costs of Enoxaparin Monotherapy Versus Unfractionated Heparin Monotherapy in Treating Acute Coronary Syndrome.
32578166	4	83	from	utilization	537:547	arg1	ACS					595:597	treating ACS	586:597	treating ACS	586:597	This study examined the differences in utilization, effectiveness, safety, and costs in treating ACS between enoxaparin and UFH monotherapy using real-world data.
32578166	1	84	theme	myocardial	321:330	arg1	infarction					332:341	myocardial infarction with	321:346	myocardial infarction with (STEMI)	321:354	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	1	84	theme	myocardial	321:330	arg1	STEMI					349:353	STEMI	349:353	STEMI	349:353	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	12	85	theme	clinical	1983:1990	arg1	outcomes					1992:1999	clinical outcomes	1983:1999	clinical outcomes	1983:1999	Improving upstream selection of anticoagulants in appropriate populations may help optimize clinical outcomes and costs.
32578166	1	86	theme	with	343:346	arg1	infarction					332:341	myocardial infarction with	321:346	myocardial infarction with (STEMI)	321:354	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	1	86	theme	with	343:346	arg1	STEMI					349:353	STEMI	349:353	STEMI	349:353	BACKGROUND Enoxaparin and unfractionated heparin (UFH) are guideline-recommended anticoagulants for patients with acute coronary syndrome (ACS), including unstable angina (UA) and myocardial infarction with (STEMI) or without ST-segment elevation (NSTEMI).
32578166	5	87	from	hospitals	692:700	arg1	data					675:678	data	675:678	data from 859 US hospitals, inpatients ≥ 18 years of age with a diagnosis of an initial episode of ACS between 2010 and 2016	675:798	METHODS Using data from 859 US hospitals, inpatients ≥ 18 years of age with a diagnosis of an initial episode of ACS between 2010 and 2016 were identified.
32578166	4	88	theme	real-world	644:653	arg1	data					655:658	real-world data	644:658	real-world data	644:658	This study examined the differences in utilization, effectiveness, safety, and costs in treating ACS between enoxaparin and UFH monotherapy using real-world data.
32683238	0	0	theme	keratin/chitosan	65:80	arg1	properties					51:60	the gel properties	43:60	the gel properties of keratin/chitosan	43:80	Effect of varied hair protein fractions on the gel properties of keratin/chitosan hydrogels for the use in tissue engineering.
32683238	5	1	theme	compressive	806:816	arg1	modulus					830:836	The various compressive and tensile modulus	794:836	The various compressive and tensile modulus of the gel	794:847	The various compressive and tensile modulus of the gel was observed based on the selection of different protein fractions as the significant gel components.
32683238	2	2	theme	hybrid	353:358	arg1	hydrogels					360:368	the hybrid hydrogels	349:368	the hybrid hydrogels	349:368	While human hair keratins could provide biological functions, chitosan could further enhance the mechanical strength of the hybrid hydrogels.
32683238	4	3	theme	study	562:566	arg1	purpose					546:552	The purpose	542:552	The purpose of this study	542:566	The purpose of this study was to investigate the role of hair protein composition, including the keratin-associated proteins (KAPs, 6-30 kDa) and keratin intermediate filaments (KIFs, 45-60 kDa) on gel characteristics of the keratin/chitosan hydrogel.
32683238	0	4	from	Effect	0:5	arg1	properties					51:60	the gel properties	43:60	the gel properties of keratin/chitosan	43:80	Effect of varied hair protein fractions on the gel properties of keratin/chitosan hydrogels for the use in tissue engineering.
32683238	3	5	theme	gel	526:528	arg1	properties					530:539	the batch-to-batch inconsistent gel properties	494:539	the batch-to-batch inconsistent gel properties	494:539	However, hair keratin is a group of natural proteins, and the uncontrolled hair protein contents in a hydrogel may lead to the batch-to-batch inconsistent gel properties.
32683238	4	6	theme	hair	599:602	arg1	composition					612:622	hair protein composition	599:622	hair protein composition	599:622	The purpose of this study was to investigate the role of hair protein composition, including the keratin-associated proteins (KAPs, 6-30 kDa) and keratin intermediate filaments (KIFs, 45-60 kDa) on gel characteristics of the keratin/chitosan hydrogel.
32683238	4	7	dep	filaments	709:717	arg1	KIFs					720:723	KIFs	720:723	KIFs	720:723	The purpose of this study was to investigate the role of hair protein composition, including the keratin-associated proteins (KAPs, 6-30 kDa) and keratin intermediate filaments (KIFs, 45-60 kDa) on gel characteristics of the keratin/chitosan hydrogel.
32683238	4	7	dep	filaments	709:717	arg1	45-60 kDa					726:734	45-60 kDa	726:734	45-60 kDa	726:734	The purpose of this study was to investigate the role of hair protein composition, including the keratin-associated proteins (KAPs, 6-30 kDa) and keratin intermediate filaments (KIFs, 45-60 kDa) on gel characteristics of the keratin/chitosan hydrogel.
32683238	3	8	theme	protein	451:457	arg1	contents					459:466	the uncontrolled hair protein contents	429:466	the uncontrolled hair protein contents in a hydrogel	429:480	However, hair keratin is a group of natural proteins, and the uncontrolled hair protein contents in a hydrogel may lead to the batch-to-batch inconsistent gel properties.
32683238	2	9	theme	biological	269:278	arg1	functions					280:288	biological functions	269:288	biological functions	269:288	While human hair keratins could provide biological functions, chitosan could further enhance the mechanical strength of the hybrid hydrogels.
32683238	5	10	theme	gel	845:847	arg1	modulus					830:836	The various compressive and tensile modulus	794:836	The various compressive and tensile modulus of the gel	794:847	The various compressive and tensile modulus of the gel was observed based on the selection of different protein fractions as the significant gel components.
32683238	6	11	theme	gel	1075:1077	arg1	properties					1079:1088	much more controllable gel properties	1052:1088	much more controllable gel properties	1052:1088	These results thus suggest a straightforward method of preparing hair keratin/chitosan hydrogel with much more controllable gel properties by merely modulating the KAPs/KIFs ratios in a gel.
32683238	6	12	theme	hair	1016:1019	arg1	hydrogel					1038:1045	hair keratin/chitosan hydrogel	1016:1045	hair keratin/chitosan hydrogel with much more controllable gel properties	1016:1088	These results thus suggest a straightforward method of preparing hair keratin/chitosan hydrogel with much more controllable gel properties by merely modulating the KAPs/KIFs ratios in a gel.
32683238	6	13	theme	KAPs/KIFs	1115:1123	arg1	ratios					1125:1130	the KAPs/KIFs ratios	1111:1130	the KAPs/KIFs ratios	1111:1130	These results thus suggest a straightforward method of preparing hair keratin/chitosan hydrogel with much more controllable gel properties by merely modulating the KAPs/KIFs ratios in a gel.
32683238	3	14	from	contents	459:466	arg1	hydrogel					473:480	a hydrogel	471:480	a hydrogel	471:480	However, hair keratin is a group of natural proteins, and the uncontrolled hair protein contents in a hydrogel may lead to the batch-to-batch inconsistent gel properties.
32683238	3	15	theme	inconsistent	513:524	arg1	properties					530:539	the batch-to-batch inconsistent gel properties	494:539	the batch-to-batch inconsistent gel properties	494:539	However, hair keratin is a group of natural proteins, and the uncontrolled hair protein contents in a hydrogel may lead to the batch-to-batch inconsistent gel properties.
32683238	1	16	theme	available	167:175	arg1	source					177:182	a readily available source	157:182	a readily available source for a hybrid hydrogel in tissue engineering	157:226	Keratin/chitosan composite is a readily available source for a hybrid hydrogel in tissue engineering.
32683238	1	16	theme	available	167:175	arg1	composite					144:152	Keratin/chitosan composite	127:152	Keratin/chitosan composite	127:152	Keratin/chitosan composite is a readily available source for a hybrid hydrogel in tissue engineering.
32683238	3	17	theme	hair	380:383	arg1	keratin					385:391	hair keratin	380:391	hair keratin	380:391	However, hair keratin is a group of natural proteins, and the uncontrolled hair protein contents in a hydrogel may lead to the batch-to-batch inconsistent gel properties.
32683238	3	17	theme	hair	380:383	arg1	group					398:402	a group	396:402	a group of natural proteins	396:422	However, hair keratin is a group of natural proteins, and the uncontrolled hair protein contents in a hydrogel may lead to the batch-to-batch inconsistent gel properties.
32683238	3	17	theme	hair	380:383	arg1	proteins					415:422	natural proteins	407:422	natural proteins	407:422	However, hair keratin is a group of natural proteins, and the uncontrolled hair protein contents in a hydrogel may lead to the batch-to-batch inconsistent gel properties.
32683238	5	18	theme	fractions	906:914	arg1	selection					875:883	the selection	871:883	the selection of different protein fractions as the significant gel components	871:948	The various compressive and tensile modulus of the gel was observed based on the selection of different protein fractions as the significant gel components.
32683238	3	19	theme	natural	407:413	arg1	proteins					415:422	natural proteins	407:422	natural proteins	407:422	However, hair keratin is a group of natural proteins, and the uncontrolled hair protein contents in a hydrogel may lead to the batch-to-batch inconsistent gel properties.
32683238	6	20	with	hydrogel	1038:1045	arg1	properties					1079:1088	much more controllable gel properties	1052:1088	much more controllable gel properties	1052:1088	These results thus suggest a straightforward method of preparing hair keratin/chitosan hydrogel with much more controllable gel properties by merely modulating the KAPs/KIFs ratios in a gel.
32683238	3	21	theme	proteins	415:422	arg1	keratin					385:391	hair keratin	380:391	hair keratin	380:391	However, hair keratin is a group of natural proteins, and the uncontrolled hair protein contents in a hydrogel may lead to the batch-to-batch inconsistent gel properties.
32683238	3	21	theme	proteins	415:422	arg1	group					398:402	a group	396:402	a group of natural proteins	396:422	However, hair keratin is a group of natural proteins, and the uncontrolled hair protein contents in a hydrogel may lead to the batch-to-batch inconsistent gel properties.
32683238	3	21	theme	proteins	415:422	arg1	proteins					415:422	natural proteins	407:422	natural proteins	407:422	However, hair keratin is a group of natural proteins, and the uncontrolled hair protein contents in a hydrogel may lead to the batch-to-batch inconsistent gel properties.
32683238	0	22	theme	hair	17:20	arg1	fractions					30:38	varied hair protein fractions	10:38	varied hair protein fractions	10:38	Effect of varied hair protein fractions on the gel properties of keratin/chitosan hydrogels for the use in tissue engineering.
32683238	4	23	theme	keratin/chitosan	767:782	arg1	hydrogel					784:791	the keratin/chitosan hydrogel	763:791	the keratin/chitosan hydrogel	763:791	The purpose of this study was to investigate the role of hair protein composition, including the keratin-associated proteins (KAPs, 6-30 kDa) and keratin intermediate filaments (KIFs, 45-60 kDa) on gel characteristics of the keratin/chitosan hydrogel.
32683238	0	24	theme	tissue	107:112	arg1	engineering					114:124	tissue engineering	107:124	tissue engineering	107:124	Effect of varied hair protein fractions on the gel properties of keratin/chitosan hydrogels for the use in tissue engineering.
32683238	5	25	theme	protein	898:904	arg1	fractions					906:914	different protein fractions	888:914	different protein fractions	888:914	The various compressive and tensile modulus of the gel was observed based on the selection of different protein fractions as the significant gel components.
32683238	3	26	theme	hair	446:449	arg1	contents					459:466	the uncontrolled hair protein contents	429:466	the uncontrolled hair protein contents in a hydrogel	429:480	However, hair keratin is a group of natural proteins, and the uncontrolled hair protein contents in a hydrogel may lead to the batch-to-batch inconsistent gel properties.
32683238	0	27	theme	varied	10:15	arg1	fractions					30:38	varied hair protein fractions	10:38	varied hair protein fractions	10:38	Effect of varied hair protein fractions on the gel properties of keratin/chitosan hydrogels for the use in tissue engineering.
32683238	3	28	theme	batch-to-batch	498:511	arg1	properties					530:539	the batch-to-batch inconsistent gel properties	494:539	the batch-to-batch inconsistent gel properties	494:539	However, hair keratin is a group of natural proteins, and the uncontrolled hair protein contents in a hydrogel may lead to the batch-to-batch inconsistent gel properties.
32683238	5	29	theme	significant	923:933	arg1	components					939:948	the significant gel components	919:948	the significant gel components	919:948	The various compressive and tensile modulus of the gel was observed based on the selection of different protein fractions as the significant gel components.
32683238	1	30	theme	hybrid	190:195	arg1	hydrogel					197:204	a hybrid hydrogel	188:204	a hybrid hydrogel in tissue engineering	188:226	Keratin/chitosan composite is a readily available source for a hybrid hydrogel in tissue engineering.
32683238	0	31	theme	fractions	30:38	arg1	Effect					0:5	Effect	0:5	Effect of varied hair protein fractions on the gel properties of keratin/chitosan	0:80	Effect of varied hair protein fractions on the gel properties of keratin/chitosan hydrogels for the use in tissue engineering.
32683238	6	32	theme	keratin/chitosan	1021:1036	arg1	hydrogel					1038:1045	hair keratin/chitosan hydrogel	1016:1045	hair keratin/chitosan hydrogel with much more controllable gel properties	1016:1088	These results thus suggest a straightforward method of preparing hair keratin/chitosan hydrogel with much more controllable gel properties by merely modulating the KAPs/KIFs ratios in a gel.
32683238	4	33	theme	keratin-associated	639:656	arg1	proteins					658:665	the keratin-associated proteins	635:665	the keratin-associated proteins (KAPs, 6-30 kDa)	635:682	The purpose of this study was to investigate the role of hair protein composition, including the keratin-associated proteins (KAPs, 6-30 kDa) and keratin intermediate filaments (KIFs, 45-60 kDa) on gel characteristics of the keratin/chitosan hydrogel.
32683238	5	34	theme	tensile	822:828	arg1	modulus					830:836	The various compressive and tensile modulus	794:836	The various compressive and tensile modulus of the gel	794:847	The various compressive and tensile modulus of the gel was observed based on the selection of different protein fractions as the significant gel components.
32683238	0	35	theme	protein	22:28	arg1	fractions					30:38	varied hair protein fractions	10:38	varied hair protein fractions	10:38	Effect of varied hair protein fractions on the gel properties of keratin/chitosan hydrogels for the use in tissue engineering.
32683238	4	36	theme	hydrogel	784:791	arg1	characteristics					744:758	gel characteristics	740:758	gel characteristics of the keratin/chitosan hydrogel	740:791	The purpose of this study was to investigate the role of hair protein composition, including the keratin-associated proteins (KAPs, 6-30 kDa) and keratin intermediate filaments (KIFs, 45-60 kDa) on gel characteristics of the keratin/chitosan hydrogel.
32683238	1	37	from	hydrogel	197:204	arg1	engineering					216:226	tissue engineering	209:226	tissue engineering	209:226	Keratin/chitosan composite is a readily available source for a hybrid hydrogel in tissue engineering.
32683238	3	38	theme	uncontrolled	433:444	arg1	contents					459:466	the uncontrolled hair protein contents	429:466	the uncontrolled hair protein contents in a hydrogel	429:480	However, hair keratin is a group of natural proteins, and the uncontrolled hair protein contents in a hydrogel may lead to the batch-to-batch inconsistent gel properties.
32683238	5	39	theme	various	798:804	arg1	modulus					830:836	The various compressive and tensile modulus	794:836	The various compressive and tensile modulus of the gel	794:847	The various compressive and tensile modulus of the gel was observed based on the selection of different protein fractions as the significant gel components.
32683238	4	40	from	role	591:594	arg1	characteristics					744:758	gel characteristics	740:758	gel characteristics of the keratin/chitosan hydrogel	740:791	The purpose of this study was to investigate the role of hair protein composition, including the keratin-associated proteins (KAPs, 6-30 kDa) and keratin intermediate filaments (KIFs, 45-60 kDa) on gel characteristics of the keratin/chitosan hydrogel.
32683238	0	41	from	use	100:102	arg1	engineering					114:124	tissue engineering	107:124	tissue engineering	107:124	Effect of varied hair protein fractions on the gel properties of keratin/chitosan hydrogels for the use in tissue engineering.
32683238	5	42	theme	different	888:896	arg1	fractions					906:914	different protein fractions	888:914	different protein fractions	888:914	The various compressive and tensile modulus of the gel was observed based on the selection of different protein fractions as the significant gel components.
32683238	4	43	dep	proteins	658:665	arg1	6-30 kDa					674:681	6-30 kDa	674:681	6-30 kDa	674:681	The purpose of this study was to investigate the role of hair protein composition, including the keratin-associated proteins (KAPs, 6-30 kDa) and keratin intermediate filaments (KIFs, 45-60 kDa) on gel characteristics of the keratin/chitosan hydrogel.
32683238	4	43	dep	proteins	658:665	arg1	KAPs					668:671	KAPs	668:671	KAPs	668:671	The purpose of this study was to investigate the role of hair protein composition, including the keratin-associated proteins (KAPs, 6-30 kDa) and keratin intermediate filaments (KIFs, 45-60 kDa) on gel characteristics of the keratin/chitosan hydrogel.
32683238	2	44	theme	mechanical	326:335	arg1	strength					337:344	the mechanical strength	322:344	the mechanical strength of the hybrid hydrogels	322:368	While human hair keratins could provide biological functions, chitosan could further enhance the mechanical strength of the hybrid hydrogels.
32683238	1	45	theme	tissue	209:214	arg1	engineering					216:226	tissue engineering	209:226	tissue engineering	209:226	Keratin/chitosan composite is a readily available source for a hybrid hydrogel in tissue engineering.
32683238	4	46	theme	gel	740:742	arg1	characteristics					744:758	gel characteristics	740:758	gel characteristics of the keratin/chitosan hydrogel	740:791	The purpose of this study was to investigate the role of hair protein composition, including the keratin-associated proteins (KAPs, 6-30 kDa) and keratin intermediate filaments (KIFs, 45-60 kDa) on gel characteristics of the keratin/chitosan hydrogel.
32683238	5	47	theme	gel	935:937	arg1	components					939:948	the significant gel components	919:948	the significant gel components	919:948	The various compressive and tensile modulus of the gel was observed based on the selection of different protein fractions as the significant gel components.
32683238	4	48	theme	keratin	688:694	arg1	filaments					709:717	keratin intermediate filaments	688:717	keratin intermediate filaments (KIFs, 45-60 kDa)	688:735	The purpose of this study was to investigate the role of hair protein composition, including the keratin-associated proteins (KAPs, 6-30 kDa) and keratin intermediate filaments (KIFs, 45-60 kDa) on gel characteristics of the keratin/chitosan hydrogel.
32683238	4	49	theme	intermediate	696:707	arg1	filaments					709:717	keratin intermediate filaments	688:717	keratin intermediate filaments (KIFs, 45-60 kDa)	688:735	The purpose of this study was to investigate the role of hair protein composition, including the keratin-associated proteins (KAPs, 6-30 kDa) and keratin intermediate filaments (KIFs, 45-60 kDa) on gel characteristics of the keratin/chitosan hydrogel.
32683238	4	50	theme	composition	612:622	arg1	role					591:594	the role	587:594	the role of hair protein composition, including the keratin-associated proteins (KAPs, 6-30 kDa) and keratin intermediate filaments (KIFs, 45-60 kDa) on gel characteristics of the keratin/chitosan hydrogel	587:791	The purpose of this study was to investigate the role of hair protein composition, including the keratin-associated proteins (KAPs, 6-30 kDa) and keratin intermediate filaments (KIFs, 45-60 kDa) on gel characteristics of the keratin/chitosan hydrogel.
32683238	2	51	theme	hair	241:244	arg1	keratins					246:253	human hair keratins	235:253	human hair keratins	235:253	While human hair keratins could provide biological functions, chitosan could further enhance the mechanical strength of the hybrid hydrogels.
32683238	4	52	theme	protein	604:610	arg1	composition					612:622	hair protein composition	599:622	hair protein composition	599:622	The purpose of this study was to investigate the role of hair protein composition, including the keratin-associated proteins (KAPs, 6-30 kDa) and keratin intermediate filaments (KIFs, 45-60 kDa) on gel characteristics of the keratin/chitosan hydrogel.
32683238	1	53	theme	Keratin/chitosan	127:142	arg1	source					177:182	a readily available source	157:182	a readily available source for a hybrid hydrogel in tissue engineering	157:226	Keratin/chitosan composite is a readily available source for a hybrid hydrogel in tissue engineering.
32683238	1	53	theme	Keratin/chitosan	127:142	arg1	composite					144:152	Keratin/chitosan composite	127:152	Keratin/chitosan composite	127:152	Keratin/chitosan composite is a readily available source for a hybrid hydrogel in tissue engineering.
32683238	6	54	theme	controllable	1062:1073	arg1	properties					1079:1088	much more controllable gel properties	1052:1088	much more controllable gel properties	1052:1088	These results thus suggest a straightforward method of preparing hair keratin/chitosan hydrogel with much more controllable gel properties by merely modulating the KAPs/KIFs ratios in a gel.
32683238	0	55	theme	gel	47:49	arg1	properties					51:60	the gel properties	43:60	the gel properties of keratin/chitosan	43:80	Effect of varied hair protein fractions on the gel properties of keratin/chitosan hydrogels for the use in tissue engineering.
32683238	2	56	theme	human	235:239	arg1	keratins					246:253	human hair keratins	235:253	human hair keratins	235:253	While human hair keratins could provide biological functions, chitosan could further enhance the mechanical strength of the hybrid hydrogels.
32683238	2	57	theme	hydrogels	360:368	arg1	strength					337:344	the mechanical strength	322:344	the mechanical strength of the hybrid hydrogels	322:368	While human hair keratins could provide biological functions, chitosan could further enhance the mechanical strength of the hybrid hydrogels.
32683238	6	58	theme	straightforward	980:994	arg1	method					996:1001	a straightforward method	978:1001	a straightforward method of preparing hair keratin/chitosan hydrogel with much more controllable gel properties by merely modulating the KAPs/KIFs ratios in a gel	978:1139	These results thus suggest a straightforward method of preparing hair keratin/chitosan hydrogel with much more controllable gel properties by merely modulating the KAPs/KIFs ratios in a gel.
32204030	0	0	theme	fibrinogen	99:108	arg1	adsorption					110:119	fibrinogen adsorption	99:119	fibrinogen adsorption	99:119	Konjac glucomannan/polyvinyl alcohol nanofibers with enhanced skin healing properties by improving fibrinogen adsorption.
32204030	6	1	theme	adsorption	876:885	arg1	rates					887:891	The fibrinogen adsorption rates	861:891	The fibrinogen adsorption rates of PVA and KGM/PVA (5:5) composites	861:927	The fibrinogen adsorption rates of PVA and KGM/PVA (5:5) composites can reach about 20% and 60%, respectively.
32204030	10	2	theme	material	1325:1332	arg1	strategy					1334:1341	a potential wound dressing material strategy	1298:1341	a potential wound dressing material strategy based on the materials design and the intrinsic properties of KGM	1298:1407	Thus, we propose a potential wound dressing material strategy based on the materials design and the intrinsic properties of KGM.
32204030	1	3	theme	healing	176:182	arg1	strategy					184:191	the effective healing strategy	162:191	the effective healing strategy to repair the wound by optimizing skin scaffold materials	162:249	Skin tissue engineering aims to develop the effective healing strategy to repair the wound by optimizing skin scaffold materials.
32204030	10	4	theme	KGM	1405:1407	arg1	properties					1391:1400	the intrinsic properties	1377:1400	the intrinsic properties of KGM	1377:1407	Thus, we propose a potential wound dressing material strategy based on the materials design and the intrinsic properties of KGM.
32204030	10	4	theme	KGM	1405:1407	arg1	design					1366:1371	the materials design	1352:1371	the materials design	1352:1371	Thus, we propose a potential wound dressing material strategy based on the materials design and the intrinsic properties of KGM.
32204030	6	5	theme	fibrinogen	865:874	arg1	rates					887:891	The fibrinogen adsorption rates	861:891	The fibrinogen adsorption rates of PVA and KGM/PVA (5:5) composites	861:927	The fibrinogen adsorption rates of PVA and KGM/PVA (5:5) composites can reach about 20% and 60%, respectively.
32204030	7	6	theme	composite	1036:1044	arg1	capacity					1010:1017	the blood adsorption capacity	989:1017	the blood adsorption capacity of KGM/PVA (5:5) composite	989:1044	The results show the blood adsorption capacity of KGM/PVA (5:5) composite can reach about 13 g/g.
32204030	10	7	theme	potential	1300:1308	arg1	strategy					1334:1341	a potential wound dressing material strategy	1298:1341	a potential wound dressing material strategy based on the materials design and the intrinsic properties of KGM	1298:1407	Thus, we propose a potential wound dressing material strategy based on the materials design and the intrinsic properties of KGM.
32204030	4	8	theme	alcohol	592:598	arg1	composites					610:619	konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites	563:619	konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites with different ratio	563:640	A series of konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites with different ratio are fabricated and their role in enhancing the skin repair is tested by in vitro cell culture and in vivo study.
32204030	10	9	theme	wound	1310:1314	arg1	strategy					1334:1341	a potential wound dressing material strategy	1298:1341	a potential wound dressing material strategy based on the materials design and the intrinsic properties of KGM	1298:1407	Thus, we propose a potential wound dressing material strategy based on the materials design and the intrinsic properties of KGM.
32204030	1	10	theme	tissue	127:132	arg1	engineering					134:144	Skin tissue engineering	122:144	Skin tissue engineering	122:144	Skin tissue engineering aims to develop the effective healing strategy to repair the wound by optimizing skin scaffold materials.
32204030	7	11	theme	5:5	1031:1033	arg1	composite					1036:1044	KGM/PVA (5:5) composite	1022:1044	KGM/PVA (5:5) composite	1022:1044	The results show the blood adsorption capacity of KGM/PVA (5:5) composite can reach about 13 g/g.
32204030	4	12	theme	cell	723:726	arg1	culture					728:734	in vitro cell culture	714:734	in vitro cell culture	714:734	A series of konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites with different ratio are fabricated and their role in enhancing the skin repair is tested by in vitro cell culture and in vivo study.
32204030	3	13	theme	dynamics	478:485	arg1	simulation					487:496	the molecular dynamics simulation	464:496	the molecular dynamics simulation	464:496	In this study, the outstanding fibrin capability of konjac glucomannan (KGM) is demonstrated by the molecular dynamics simulation and confirmed by the protein adsorption experiments.
32204030	4	14	theme	in	714:715	arg1	culture					728:734	in vitro cell culture	714:734	in vitro cell culture	714:734	A series of konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites with different ratio are fabricated and their role in enhancing the skin repair is tested by in vitro cell culture and in vivo study.
32204030	3	15	theme	adsorption	527:536	arg1	experiments					538:548	the protein adsorption experiments	515:548	the protein adsorption experiments	515:548	In this study, the outstanding fibrin capability of konjac glucomannan (KGM) is demonstrated by the molecular dynamics simulation and confirmed by the protein adsorption experiments.
32204030	8	16	theme	KGM/PVA	1149:1155	arg1	composite					1163:1171	KGM/PVA (5:5) composite	1149:1171	KGM/PVA (5:5) composite	1149:1171	After 7 days of cell culture, the optical density values of 3T3 fibroblasts on KGM/PVA (5:5) composite could reach 0.8.
32204030	8	17	theme	fibroblasts	1134:1144	arg1	values					1120:1125	the optical density values	1100:1125	the optical density values of 3T3 fibroblasts on KGM/PVA (5:5) composite	1100:1171	After 7 days of cell culture, the optical density values of 3T3 fibroblasts on KGM/PVA (5:5) composite could reach 0.8.
32204030	4	18	theme	KGM/PVA	601:607	arg1	composites					610:619	konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites	563:619	konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites with different ratio	563:640	A series of konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites with different ratio are fabricated and their role in enhancing the skin repair is tested by in vitro cell culture and in vivo study.
32204030	7	19	theme	KGM/PVA	1022:1028	arg1	composite					1036:1044	KGM/PVA (5:5) composite	1022:1044	KGM/PVA (5:5) composite	1022:1044	The results show the blood adsorption capacity of KGM/PVA (5:5) composite can reach about 13 g/g.
32204030	10	20	theme	materials	1356:1364	arg1	design					1366:1371	the materials design	1352:1371	the materials design	1352:1371	Thus, we propose a potential wound dressing material strategy based on the materials design and the intrinsic properties of KGM.
32204030	3	21	theme	outstanding	387:397	arg1	capability					406:415	the outstanding fibrin capability	383:415	the outstanding fibrin capability of konjac glucomannan (KGM)	383:443	In this study, the outstanding fibrin capability of konjac glucomannan (KGM) is demonstrated by the molecular dynamics simulation and confirmed by the protein adsorption experiments.
32204030	8	22	theme	3T3	1130:1132	arg1	fibroblasts					1134:1144	3T3 fibroblasts	1130:1144	3T3 fibroblasts	1130:1144	After 7 days of cell culture, the optical density values of 3T3 fibroblasts on KGM/PVA (5:5) composite could reach 0.8.
32204030	0	23	theme	glucomannan/polyvinyl	7:27	arg1	alcohol					29:35	Konjac glucomannan/polyvinyl alcohol	0:35	Konjac glucomannan/polyvinyl alcohol	0:35	Konjac glucomannan/polyvinyl alcohol nanofibers with enhanced skin healing properties by improving fibrinogen adsorption.
32204030	8	24	theme	cell	1086:1089	arg1	culture					1091:1097	cell culture	1086:1097	cell culture	1086:1097	After 7 days of cell culture, the optical density values of 3T3 fibroblasts on KGM/PVA (5:5) composite could reach 0.8.
32204030	7	25	theme	adsorption	999:1008	arg1	capacity					1010:1017	the blood adsorption capacity	989:1017	the blood adsorption capacity of KGM/PVA (5:5) composite	989:1044	The results show the blood adsorption capacity of KGM/PVA (5:5) composite can reach about 13 g/g.
32204030	8	26	from	values	1120:1125	arg1	composite					1163:1171	KGM/PVA (5:5) composite	1149:1171	KGM/PVA (5:5) composite	1149:1171	After 7 days of cell culture, the optical density values of 3T3 fibroblasts on KGM/PVA (5:5) composite could reach 0.8.
32204030	3	27	theme	glucomannan	427:437	arg1	capability					406:415	the outstanding fibrin capability	383:415	the outstanding fibrin capability of konjac glucomannan (KGM)	383:443	In this study, the outstanding fibrin capability of konjac glucomannan (KGM) is demonstrated by the molecular dynamics simulation and confirmed by the protein adsorption experiments.
32204030	0	28	theme	Konjac	0:5	arg1	alcohol					29:35	Konjac glucomannan/polyvinyl alcohol	0:35	Konjac glucomannan/polyvinyl alcohol	0:35	Konjac glucomannan/polyvinyl alcohol nanofibers with enhanced skin healing properties by improving fibrinogen adsorption.
32204030	6	29	theme	composites	918:927	arg1	rates					887:891	The fibrinogen adsorption rates	861:891	The fibrinogen adsorption rates of PVA and KGM/PVA (5:5) composites	861:927	The fibrinogen adsorption rates of PVA and KGM/PVA (5:5) composites can reach about 20% and 60%, respectively.
32204030	8	30	theme	density	1112:1118	arg1	values					1120:1125	the optical density values	1100:1125	the optical density values of 3T3 fibroblasts on KGM/PVA (5:5) composite	1100:1171	After 7 days of cell culture, the optical density values of 3T3 fibroblasts on KGM/PVA (5:5) composite could reach 0.8.
32204030	10	31	theme	dressing	1316:1323	arg1	strategy					1334:1341	a potential wound dressing material strategy	1298:1341	a potential wound dressing material strategy based on the materials design and the intrinsic properties of KGM	1298:1407	Thus, we propose a potential wound dressing material strategy based on the materials design and the intrinsic properties of KGM.
32204030	4	32	theme	composites	610:619	arg1	series					553:558	A series	551:558	A series of konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites with different ratio	551:640	A series of konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites with different ratio are fabricated and their role in enhancing the skin repair is tested by in vitro cell culture and in vivo study.
32204030	9	33	theme	composites	1223:1232	arg1	properties					1205:1214	The mechanical properties	1190:1214	The mechanical properties of the composites	1190:1232	The mechanical properties of the composites are also verified to meet the practical needs.
32204030	1	34	theme	Skin	122:125	arg1	engineering					134:144	Skin tissue engineering	122:144	Skin tissue engineering	122:144	Skin tissue engineering aims to develop the effective healing strategy to repair the wound by optimizing skin scaffold materials.
32204030	8	35	theme	5:5	1158:1160	arg1	composite					1163:1171	KGM/PVA (5:5) composite	1149:1171	KGM/PVA (5:5) composite	1149:1171	After 7 days of cell culture, the optical density values of 3T3 fibroblasts on KGM/PVA (5:5) composite could reach 0.8.
32204030	4	36	theme	glucomannan/polyvinyl	570:590	arg1	composites					610:619	konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites	563:619	konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites with different ratio	563:640	A series of konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites with different ratio are fabricated and their role in enhancing the skin repair is tested by in vitro cell culture and in vivo study.
32204030	7	37	theme	blood	993:997	arg1	capacity					1010:1017	the blood adsorption capacity	989:1017	the blood adsorption capacity of KGM/PVA (5:5) composite	989:1044	The results show the blood adsorption capacity of KGM/PVA (5:5) composite can reach about 13 g/g.
32204030	2	38	theme	important	307:315	arg1	role					317:320	an important role	304:320	an important role	304:320	During the skin wound healing process, fibrin plays an important role due to the specific blood coagulation effect.
32204030	4	39	dep	in	740:741	arg1	vivo					743:746	vivo	743:746	vivo	743:746	A series of konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites with different ratio are fabricated and their role in enhancing the skin repair is tested by in vitro cell culture and in vivo study.
32204030	0	40	theme	enhanced	53:60	arg1	properties					75:84	enhanced skin healing properties	53:84	enhanced skin healing properties	53:84	Konjac glucomannan/polyvinyl alcohol nanofibers with enhanced skin healing properties by improving fibrinogen adsorption.
32204030	4	41	theme	konjac	563:568	arg1	composites					610:619	konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites	563:619	konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites with different ratio	563:640	A series of konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites with different ratio are fabricated and their role in enhancing the skin repair is tested by in vitro cell culture and in vivo study.
32204030	9	42	theme	mechanical	1194:1203	arg1	properties					1205:1214	The mechanical properties	1190:1214	The mechanical properties of the composites	1190:1232	The mechanical properties of the composites are also verified to meet the practical needs.
32204030	1	43	theme	skin	227:230	arg1	materials					241:249	skin scaffold materials	227:249	skin scaffold materials	227:249	Skin tissue engineering aims to develop the effective healing strategy to repair the wound by optimizing skin scaffold materials.
32204030	3	44	theme	konjac	420:425	arg1	KGM					440:442	KGM	440:442	KGM	440:442	In this study, the outstanding fibrin capability of konjac glucomannan (KGM) is demonstrated by the molecular dynamics simulation and confirmed by the protein adsorption experiments.
32204030	3	44	theme	konjac	420:425	arg1	glucomannan					427:437	konjac glucomannan	420:437	konjac glucomannan (KGM)	420:443	In this study, the outstanding fibrin capability of konjac glucomannan (KGM) is demonstrated by the molecular dynamics simulation and confirmed by the protein adsorption experiments.
32204030	6	45	theme	KGM/PVA	904:910	arg1	composites					918:927	PVA and KGM/PVA (5:5) composites	896:927	PVA and KGM/PVA (5:5) composites	896:927	The fibrinogen adsorption rates of PVA and KGM/PVA (5:5) composites can reach about 20% and 60%, respectively.
32204030	5	46	theme	adsorption	765:774	arg1	energy					776:781	adsorption energy	765:781	adsorption energy	765:781	The Eads (adsorption energy) between fibrin and KGM is about 30% larger than that between fibrin and PVA.
32204030	5	46	theme	adsorption	765:774	arg1	Eads					759:762	The Eads	755:762	The Eads (adsorption energy) between fibrin and KGM	755:805	The Eads (adsorption energy) between fibrin and KGM is about 30% larger than that between fibrin and PVA.
32204030	9	47	dep	practical	1264:1272	arg1	needs					1274:1278	needs	1274:1278	needs	1274:1278	The mechanical properties of the composites are also verified to meet the practical needs.
32204030	1	48	theme	scaffold	232:239	arg1	materials					241:249	skin scaffold materials	227:249	skin scaffold materials	227:249	Skin tissue engineering aims to develop the effective healing strategy to repair the wound by optimizing skin scaffold materials.
32204030	0	49	theme	healing	67:73	arg1	properties					75:84	enhanced skin healing properties	53:84	enhanced skin healing properties	53:84	Konjac glucomannan/polyvinyl alcohol nanofibers with enhanced skin healing properties by improving fibrinogen adsorption.
32204030	8	50	theme	optical	1104:1110	arg1	values					1120:1125	the optical density values	1100:1125	the optical density values of 3T3 fibroblasts on KGM/PVA (5:5) composite	1100:1171	After 7 days of cell culture, the optical density values of 3T3 fibroblasts on KGM/PVA (5:5) composite could reach 0.8.
32204030	7	51	dep	show	984:987	arg1	reach					1050:1054	reach	1050:1054	show the blood adsorption capacity of KGM/PVA (5:5) composite can reach about 13 g/g	984:1067	The results show the blood adsorption capacity of KGM/PVA (5:5) composite can reach about 13 g/g.
32204030	2	52	theme	coagulation	348:358	arg1	effect					360:365	the specific blood coagulation effect	329:365	the specific blood coagulation effect	329:365	During the skin wound healing process, fibrin plays an important role due to the specific blood coagulation effect.
32204030	0	53	theme	skin	62:65	arg1	properties					75:84	enhanced skin healing properties	53:84	enhanced skin healing properties	53:84	Konjac glucomannan/polyvinyl alcohol nanofibers with enhanced skin healing properties by improving fibrinogen adsorption.
32204030	6	54	theme	PVA	896:898	arg1	composites					918:927	PVA and KGM/PVA (5:5) composites	896:927	PVA and KGM/PVA (5:5) composites	896:927	The fibrinogen adsorption rates of PVA and KGM/PVA (5:5) composites can reach about 20% and 60%, respectively.
32204030	4	55	with	composites	610:619	arg1	ratio					636:640	different ratio	626:640	different ratio	626:640	A series of konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites with different ratio are fabricated and their role in enhancing the skin repair is tested by in vitro cell culture and in vivo study.
32204030	2	56	theme	blood	342:346	arg1	effect					360:365	the specific blood coagulation effect	329:365	the specific blood coagulation effect	329:365	During the skin wound healing process, fibrin plays an important role due to the specific blood coagulation effect.
32204030	8	57	theme	culture	1091:1097	arg1	7 days					1076:1081	7 days	1076:1081	7 days of cell culture	1076:1097	After 7 days of cell culture, the optical density values of 3T3 fibroblasts on KGM/PVA (5:5) composite could reach 0.8.
32204030	4	58	theme	skin	689:692	arg1	repair					694:699	the skin repair	685:699	the skin repair	685:699	A series of konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites with different ratio are fabricated and their role in enhancing the skin repair is tested by in vitro cell culture and in vivo study.
32204030	2	59	theme	specific	333:340	arg1	effect					360:365	the specific blood coagulation effect	329:365	the specific blood coagulation effect	329:365	During the skin wound healing process, fibrin plays an important role due to the specific blood coagulation effect.
32204030	4	60	dep	in	714:715	arg1	vitro					717:721	vitro	717:721	vitro	717:721	A series of konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites with different ratio are fabricated and their role in enhancing the skin repair is tested by in vitro cell culture and in vivo study.
32204030	10	61	theme	intrinsic	1381:1389	arg1	properties					1391:1400	the intrinsic properties	1377:1400	the intrinsic properties of KGM	1377:1407	Thus, we propose a potential wound dressing material strategy based on the materials design and the intrinsic properties of KGM.
32204030	2	62	theme	healing	274:280	arg1	process					282:288	the skin wound healing process	259:288	the skin wound healing process	259:288	During the skin wound healing process, fibrin plays an important role due to the specific blood coagulation effect.
32204030	4	63	theme	in	740:741	arg1	study					748:752	in vivo study	740:752	in vivo study	740:752	A series of konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites with different ratio are fabricated and their role in enhancing the skin repair is tested by in vitro cell culture and in vivo study.
32204030	1	64	theme	effective	166:174	arg1	strategy					184:191	the effective healing strategy	162:191	the effective healing strategy to repair the wound by optimizing skin scaffold materials	162:249	Skin tissue engineering aims to develop the effective healing strategy to repair the wound by optimizing skin scaffold materials.
32204030	3	65	theme	molecular	468:476	arg1	simulation					487:496	the molecular dynamics simulation	464:496	the molecular dynamics simulation	464:496	In this study, the outstanding fibrin capability of konjac glucomannan (KGM) is demonstrated by the molecular dynamics simulation and confirmed by the protein adsorption experiments.
32204030	2	66	theme	wound	268:272	arg1	healing					274:280	skin wound healing	263:280	the skin wound healing process	259:288	During the skin wound healing process, fibrin plays an important role due to the specific blood coagulation effect.
32204030	3	67	theme	fibrin	399:404	arg1	capability					406:415	the outstanding fibrin capability	383:415	the outstanding fibrin capability of konjac glucomannan (KGM)	383:443	In this study, the outstanding fibrin capability of konjac glucomannan (KGM) is demonstrated by the molecular dynamics simulation and confirmed by the protein adsorption experiments.
32204030	2	68	theme	skin	263:266	arg1	healing					274:280	skin wound healing	263:280	the skin wound healing process	259:288	During the skin wound healing process, fibrin plays an important role due to the specific blood coagulation effect.
32204030	3	69	theme	protein	519:525	arg1	experiments					538:548	the protein adsorption experiments	515:548	the protein adsorption experiments	515:548	In this study, the outstanding fibrin capability of konjac glucomannan (KGM) is demonstrated by the molecular dynamics simulation and confirmed by the protein adsorption experiments.
32204030	4	70	theme	different	626:634	arg1	ratio					636:640	different ratio	626:640	different ratio	626:640	A series of konjac glucomannan/polyvinyl alcohol (KGM/PVA) composites with different ratio are fabricated and their role in enhancing the skin repair is tested by in vitro cell culture and in vivo study.
32223230	7	0	theme	coupling	1294:1301	arg1	reactions					1303:1311	coupling reactions	1294:1311	coupling reactions	1294:1311	Subsequently, we tested the ability of the as-made ChsNCs as a biomass-based catalyst support for Au nanoparticles (NPs) using the 4-nitrophenol reduction and the aldehyde-amine-alkyne (A3) coupling reactions to demonstrate its capabilities in regard to the ones of cellulose nanocrystals (CNCs).
32223230	9	1	from	site	1759:1762	arg1	bionano-support					1771:1785	the bionano-support	1767:1785	the bionano-support	1767:1785	Spectroscopic and imaging techniques confirmed the importance of precisely controlling the redox state of Au as it is being deposited to afford a highly disperse active site on the bionano-support.
32223230	6	2	theme	carboxylated	1073:1084	arg1	ChNCs					1086:1090	these carboxylated ChNCs	1067:1090	these carboxylated ChNCs	1067:1090	Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy were used to determine the morphology and composition of these carboxylated ChNCs and ChsNCs.
32223230	3	3	from	chitin	515:520	arg1	cleavage					483:490	partial cleavage	475:490	partial cleavage of glycosidic bonds in chitin by ammonium persulfate	475:543	Carboxylated chitin nanocrystals (ChNCs) were initially prepared through partial cleavage of glycosidic bonds in chitin by ammonium persulfate, with concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs.
32223230	1	4	theme	high	236:239	arg1	degree					241:246	high degree	236:246	high degree of deacetylation (DDA)	236:269	In this study, we demonstrate for the first time the fabrication of carboxylated chitosan nanocrystals (ChsNC) with high degree of deacetylation (DDA) at >80% and narrow size distribution.
32223230	0	5	theme	Superior	73:80	arg1	Support					82:88	Superior Support	73:88	Superior Support for Gold-Catalyzed Reactions	73:117	Carboxylated Chitosan Nanocrystals: A Synthetic Route and Application as Superior Support for Gold-Catalyzed Reactions.
32223230	3	6	from	cleavage	483:490	arg1	chitin					515:520	chitin	515:520	chitin	515:520	Carboxylated chitin nanocrystals (ChNCs) were initially prepared through partial cleavage of glycosidic bonds in chitin by ammonium persulfate, with concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs.
32223230	8	7	theme	frequency	1465:1473	arg1	value					1481:1485	the highest turnover frequency (TOF) value	1444:1485	the highest turnover frequency (TOF) value for the 4-nitrophenol reduction reported for all Au-based catalysts supported on carbon-based systems	1444:1587	In particular, Au NPs over ChsNCs featured the highest turnover frequency (TOF) value for the 4-nitrophenol reduction reported for all Au-based catalysts supported on carbon-based systems.
32223230	6	8	theme	infrared	981:988	arg1	spectroscopy					997:1008	Fourier-transform infrared (FTIR) spectroscopy	963:1008	Fourier-transform infrared (FTIR) spectroscopy	963:1008	Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy were used to determine the morphology and composition of these carboxylated ChNCs and ChsNCs.
32223230	0	9	theme	Gold-Catalyzed	94:107	arg1	Reactions					109:117	Gold-Catalyzed Reactions	94:117	Gold-Catalyzed Reactions	94:117	Carboxylated Chitosan Nanocrystals: A Synthetic Route and Application as Superior Support for Gold-Catalyzed Reactions.
32223230	6	10	theme	electron	907:914	arg1	microscopy					916:925	Transmission electron microscopy	894:925	Transmission electron microscopy (TEM)	894:931	Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy were used to determine the morphology and composition of these carboxylated ChNCs and ChsNCs.
32223230	6	10	theme	electron	907:914	arg1	TEM					928:930	TEM	928:930	TEM	928:930	Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy were used to determine the morphology and composition of these carboxylated ChNCs and ChsNCs.
32223230	6	11	dep	morphology	1037:1046	arg1	the					1033:1035	the	1033:1035	the	1033:1035	Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy were used to determine the morphology and composition of these carboxylated ChNCs and ChsNCs.
32223230	6	12	theme	Fourier-transform	963:979	arg1	spectroscopy					997:1008	Fourier-transform infrared (FTIR) spectroscopy	963:1008	Fourier-transform infrared (FTIR) spectroscopy	963:1008	Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy were used to determine the morphology and composition of these carboxylated ChNCs and ChsNCs.
32223230	3	13	from	groups	625:630	arg1	surface					639:645	the surface	635:645	the surface of the ChNCs	635:658	Carboxylated chitin nanocrystals (ChNCs) were initially prepared through partial cleavage of glycosidic bonds in chitin by ammonium persulfate, with concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs.
32223230	6	14	theme	Transmission	894:905	arg1	microscopy					916:925	Transmission electron microscopy	894:925	Transmission electron microscopy (TEM)	894:931	Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy were used to determine the morphology and composition of these carboxylated ChNCs and ChsNCs.
32223230	6	14	theme	Transmission	894:905	arg1	TEM					928:930	TEM	928:930	TEM	928:930	Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy were used to determine the morphology and composition of these carboxylated ChNCs and ChsNCs.
32223230	9	15	theme	disperse	1743:1750	arg1	site					1759:1762	a highly disperse active site	1734:1762	a highly disperse active site on the bionano-support	1734:1785	Spectroscopic and imaging techniques confirmed the importance of precisely controlling the redox state of Au as it is being deposited to afford a highly disperse active site on the bionano-support.
32223230	3	16	theme	ammonium	525:532	arg1	persulfate					534:543	ammonium persulfate	525:543	ammonium persulfate	525:543	Carboxylated chitin nanocrystals (ChNCs) were initially prepared through partial cleavage of glycosidic bonds in chitin by ammonium persulfate, with concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs.
32223230	1	17	theme	deacetylation	251:263	arg1	degree					241:246	high degree	236:246	high degree of deacetylation (DDA)	236:269	In this study, we demonstrate for the first time the fabrication of carboxylated chitosan nanocrystals (ChsNC) with high degree of deacetylation (DDA) at >80% and narrow size distribution.
32223230	9	18	theme	active	1752:1757	arg1	site					1759:1762	a highly disperse active site	1734:1762	a highly disperse active site on the bionano-support	1734:1785	Spectroscopic and imaging techniques confirmed the importance of precisely controlling the redox state of Au as it is being deposited to afford a highly disperse active site on the bionano-support.
32223230	8	19	theme	TOF	1476:1478	arg1	value					1481:1485	the highest turnover frequency (TOF) value	1444:1485	the highest turnover frequency (TOF) value for the 4-nitrophenol reduction reported for all Au-based catalysts supported on carbon-based systems	1444:1587	In particular, Au NPs over ChsNCs featured the highest turnover frequency (TOF) value for the 4-nitrophenol reduction reported for all Au-based catalysts supported on carbon-based systems.
32223230	5	20	theme	resulting	820:828	arg1	nanocrystals					830:841	The resulting nanocrystals	816:841	The resulting nanocrystals	816:841	The resulting nanocrystals feature both carboxyl and amino functional groups.
32223230	1	21	theme	carboxylated	188:199	arg1	ChsNC					224:228	ChsNC	224:228	ChsNC	224:228	In this study, we demonstrate for the first time the fabrication of carboxylated chitosan nanocrystals (ChsNC) with high degree of deacetylation (DDA) at >80% and narrow size distribution.
32223230	1	21	theme	carboxylated	188:199	arg1	nanocrystals					210:221	carboxylated chitosan nanocrystals	188:221	carboxylated chitosan nanocrystals (ChsNC)	188:229	In this study, we demonstrate for the first time the fabrication of carboxylated chitosan nanocrystals (ChsNC) with high degree of deacetylation (DDA) at >80% and narrow size distribution.
32223230	7	22	dep	reduction	1249:1257	arg1	reactions					1303:1311	coupling reactions	1294:1311	coupling reactions	1294:1311	Subsequently, we tested the ability of the as-made ChsNCs as a biomass-based catalyst support for Au nanoparticles (NPs) using the 4-nitrophenol reduction and the aldehyde-amine-alkyne (A3) coupling reactions to demonstrate its capabilities in regard to the ones of cellulose nanocrystals (CNCs).
32223230	1	23	theme	first	158:162	arg1	time					164:167	the first time	154:167	the first time	154:167	In this study, we demonstrate for the first time the fabrication of carboxylated chitosan nanocrystals (ChsNC) with high degree of deacetylation (DDA) at >80% and narrow size distribution.
32223230	7	24	theme	biomass-based	1167:1179	arg1	ability					1132:1138	the ability	1128:1138	the ability of the as-made ChsNCs	1128:1160	Subsequently, we tested the ability of the as-made ChsNCs as a biomass-based catalyst support for Au nanoparticles (NPs) using the 4-nitrophenol reduction and the aldehyde-amine-alkyne (A3) coupling reactions to demonstrate its capabilities in regard to the ones of cellulose nanocrystals (CNCs).
32223230	7	24	theme	biomass-based	1167:1179	arg1	catalyst					1181:1188	a biomass-based catalyst	1165:1188	a biomass-based catalyst support for Au nanoparticles (NPs) using the 4-nitrophenol reduction and the aldehyde-amine-alkyne (A3) coupling reactions to demonstrate its capabilities in regard to the ones of cellulose nanocrystals (CNCs)	1165:1398	Subsequently, we tested the ability of the as-made ChsNCs as a biomass-based catalyst support for Au nanoparticles (NPs) using the 4-nitrophenol reduction and the aldehyde-amine-alkyne (A3) coupling reactions to demonstrate its capabilities in regard to the ones of cellulose nanocrystals (CNCs).
32223230	4	25	theme	NaBH4	756:760	arg1	presence					744:751	the presence	740:751	the presence of NaBH4 to preserve the nanorod structure of the biomaterial	740:813	ChsNCs were subsequently prepared using an alkaline deacetylation procedure in the presence of NaBH4 to preserve the nanorod structure of the biomaterial.
32223230	2	26	theme	sustainable	346:356	arg1	material					366:373	a sustainable support material	344:373	a sustainable support material for metal-based catalysts	344:399	We also studied its application as a sustainable support material for metal-based catalysts.
32223230	2	26	theme	sustainable	346:356	arg1	application					329:339	its application	325:339	its application	325:339	We also studied its application as a sustainable support material for metal-based catalysts.
32223230	0	27	theme	Chitosan	13:20	arg1	Nanocrystals					22:33	Carboxylated Chitosan Nanocrystals	0:33	Carboxylated Chitosan Nanocrystals: A Synthetic Route and Application as Superior Support for Gold-Catalyzed Reactions.	0:118	Carboxylated Chitosan Nanocrystals: A Synthetic Route and Application as Superior Support for Gold-Catalyzed Reactions.
32223230	0	27	theme	Chitosan	13:20	arg1	Application					58:68	Application	58:68	Application as Superior Support for Gold-Catalyzed Reactions	58:117	Carboxylated Chitosan Nanocrystals: A Synthetic Route and Application as Superior Support for Gold-Catalyzed Reactions.
32223230	0	27	theme	Chitosan	13:20	arg1	Route					48:52	A Synthetic Route	36:52	A Synthetic Route	36:52	Carboxylated Chitosan Nanocrystals: A Synthetic Route and Application as Superior Support for Gold-Catalyzed Reactions.
32223230	7	28	theme	nanocrystals	1380:1391	arg1	ones					1362:1365	the ones	1358:1365	the ones of cellulose nanocrystals (CNCs)	1358:1398	Subsequently, we tested the ability of the as-made ChsNCs as a biomass-based catalyst support for Au nanoparticles (NPs) using the 4-nitrophenol reduction and the aldehyde-amine-alkyne (A3) coupling reactions to demonstrate its capabilities in regard to the ones of cellulose nanocrystals (CNCs).
32223230	7	29	theme	4-nitrophenol	1235:1247	arg1	reduction					1249:1257	the 4-nitrophenol reduction	1231:1257	the 4-nitrophenol reduction	1231:1257	Subsequently, we tested the ability of the as-made ChsNCs as a biomass-based catalyst support for Au nanoparticles (NPs) using the 4-nitrophenol reduction and the aldehyde-amine-alkyne (A3) coupling reactions to demonstrate its capabilities in regard to the ones of cellulose nanocrystals (CNCs).
32223230	0	30	theme	Carboxylated	0:11	arg1	Nanocrystals					22:33	Carboxylated Chitosan Nanocrystals	0:33	Carboxylated Chitosan Nanocrystals: A Synthetic Route and Application as Superior Support for Gold-Catalyzed Reactions.	0:118	Carboxylated Chitosan Nanocrystals: A Synthetic Route and Application as Superior Support for Gold-Catalyzed Reactions.
32223230	0	30	theme	Carboxylated	0:11	arg1	Application					58:68	Application	58:68	Application as Superior Support for Gold-Catalyzed Reactions	58:117	Carboxylated Chitosan Nanocrystals: A Synthetic Route and Application as Superior Support for Gold-Catalyzed Reactions.
32223230	0	30	theme	Carboxylated	0:11	arg1	Route					48:52	A Synthetic Route	36:52	A Synthetic Route	36:52	Carboxylated Chitosan Nanocrystals: A Synthetic Route and Application as Superior Support for Gold-Catalyzed Reactions.
32223230	8	31	theme	4-nitrophenol	1495:1507	arg1	reduction					1509:1517	the 4-nitrophenol reduction	1491:1517	the 4-nitrophenol reduction reported for all Au-based catalysts supported on carbon-based systems	1491:1587	In particular, Au NPs over ChsNCs featured the highest turnover frequency (TOF) value for the 4-nitrophenol reduction reported for all Au-based catalysts supported on carbon-based systems.
32223230	9	32	theme	Spectroscopic	1590:1602	arg1	techniques					1616:1625	Spectroscopic and imaging techniques	1590:1625	techniques	1616:1625	Spectroscopic and imaging techniques confirmed the importance of precisely controlling the redox state of Au as it is being deposited to afford a highly disperse active site on the bionano-support.
32223230	3	33	theme	concurrent	551:560	arg1	oxidation					562:570	concurrent oxidation	551:570	concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs	551:658	Carboxylated chitin nanocrystals (ChNCs) were initially prepared through partial cleavage of glycosidic bonds in chitin by ammonium persulfate, with concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs.
32223230	3	34	theme	Carboxylated	402:413	arg1	ChNCs					436:440	ChNCs	436:440	ChNCs	436:440	Carboxylated chitin nanocrystals (ChNCs) were initially prepared through partial cleavage of glycosidic bonds in chitin by ammonium persulfate, with concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs.
32223230	3	34	theme	Carboxylated	402:413	arg1	nanocrystals					422:433	Carboxylated chitin nanocrystals	402:433	Carboxylated chitin nanocrystals (ChNCs)	402:441	Carboxylated chitin nanocrystals (ChNCs) were initially prepared through partial cleavage of glycosidic bonds in chitin by ammonium persulfate, with concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs.
32223230	8	35	theme	carbon-based	1568:1579	arg1	systems					1581:1587	carbon-based systems	1568:1587	carbon-based systems	1568:1587	In particular, Au NPs over ChsNCs featured the highest turnover frequency (TOF) value for the 4-nitrophenol reduction reported for all Au-based catalysts supported on carbon-based systems.
32223230	4	36	theme	deacetylation	713:725	arg1	procedure					727:735	an alkaline deacetylation procedure	701:735	an alkaline deacetylation procedure	701:735	ChsNCs were subsequently prepared using an alkaline deacetylation procedure in the presence of NaBH4 to preserve the nanorod structure of the biomaterial.
32223230	9	37	theme	redox	1681:1685	arg1	state					1687:1691	the redox state	1677:1691	the redox state of Au as it is being deposited to afford a highly disperse active site on the bionano-support	1677:1785	Spectroscopic and imaging techniques confirmed the importance of precisely controlling the redox state of Au as it is being deposited to afford a highly disperse active site on the bionano-support.
32223230	3	38	from	bonds	506:510	arg1	chitin					515:520	chitin	515:520	chitin	515:520	Carboxylated chitin nanocrystals (ChNCs) were initially prepared through partial cleavage of glycosidic bonds in chitin by ammonium persulfate, with concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs.
32223230	6	39	used	used	1015:1018	arg2	TEM					928:930	TEM	928:930	TEM	928:930	Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy were used to determine the morphology and composition of these carboxylated ChNCs and ChsNCs.
32223230	6	39	used	used	1015:1018	arg2	microscopy					916:925	Transmission electron microscopy	894:925	Transmission electron microscopy (TEM)	894:931	Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy were used to determine the morphology and composition of these carboxylated ChNCs and ChsNCs.
32223230	6	39	used	used	1015:1018	arg2	spectroscopy					997:1008	Fourier-transform infrared (FTIR) spectroscopy	963:1008	Fourier-transform infrared (FTIR) spectroscopy	963:1008	Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy were used to determine the morphology and composition of these carboxylated ChNCs and ChsNCs.
32223230	6	39	used	used	1015:1018	arg2	diffraction					940:950	X-ray diffraction	934:950	X-ray diffraction (XRD)	934:956	Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy were used to determine the morphology and composition of these carboxylated ChNCs and ChsNCs.
32223230	6	39	used	used	1015:1018	arg2	XRD					953:955	XRD	953:955	XRD	953:955	Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy were used to determine the morphology and composition of these carboxylated ChNCs and ChsNCs.
32223230	4	40	theme	nanorod	778:784	arg1	structure					786:794	the nanorod structure	774:794	the nanorod structure of the biomaterial	774:813	ChsNCs were subsequently prepared using an alkaline deacetylation procedure in the presence of NaBH4 to preserve the nanorod structure of the biomaterial.
32223230	7	41	theme	as-made	1147:1153	arg1	ChsNCs					1155:1160	the as-made ChsNCs	1143:1160	the as-made ChsNCs	1143:1160	Subsequently, we tested the ability of the as-made ChsNCs as a biomass-based catalyst support for Au nanoparticles (NPs) using the 4-nitrophenol reduction and the aldehyde-amine-alkyne (A3) coupling reactions to demonstrate its capabilities in regard to the ones of cellulose nanocrystals (CNCs).
32223230	7	42	theme	cellulose	1370:1378	arg1	nanocrystals					1380:1391	cellulose nanocrystals	1370:1391	cellulose nanocrystals (CNCs)	1370:1398	Subsequently, we tested the ability of the as-made ChsNCs as a biomass-based catalyst support for Au nanoparticles (NPs) using the 4-nitrophenol reduction and the aldehyde-amine-alkyne (A3) coupling reactions to demonstrate its capabilities in regard to the ones of cellulose nanocrystals (CNCs).
32223230	7	42	theme	cellulose	1370:1378	arg1	CNCs					1394:1397	CNCs	1394:1397	CNCs	1394:1397	Subsequently, we tested the ability of the as-made ChsNCs as a biomass-based catalyst support for Au nanoparticles (NPs) using the 4-nitrophenol reduction and the aldehyde-amine-alkyne (A3) coupling reactions to demonstrate its capabilities in regard to the ones of cellulose nanocrystals (CNCs).
32223230	2	43	theme	metal-based	379:389	arg1	catalysts					391:399	metal-based catalysts	379:399	metal-based catalysts	379:399	We also studied its application as a sustainable support material for metal-based catalysts.
32223230	0	44	theme	Synthetic	38:46	arg1	Nanocrystals					22:33	Carboxylated Chitosan Nanocrystals	0:33	Carboxylated Chitosan Nanocrystals: A Synthetic Route and Application as Superior Support for Gold-Catalyzed Reactions.	0:118	Carboxylated Chitosan Nanocrystals: A Synthetic Route and Application as Superior Support for Gold-Catalyzed Reactions.
32223230	0	44	theme	Synthetic	38:46	arg1	Route					48:52	A Synthetic Route	36:52	A Synthetic Route	36:52	Carboxylated Chitosan Nanocrystals: A Synthetic Route and Application as Superior Support for Gold-Catalyzed Reactions.
32223230	8	45	theme	Au-based	1536:1543	arg1	catalysts					1545:1553	all Au-based catalysts	1532:1553	all Au-based catalysts supported on carbon-based systems	1532:1587	In particular, Au NPs over ChsNCs featured the highest turnover frequency (TOF) value for the 4-nitrophenol reduction reported for all Au-based catalysts supported on carbon-based systems.
32223230	7	46	theme	ChsNCs	1155:1160	arg1	catalyst					1181:1188	a biomass-based catalyst	1165:1188	a biomass-based catalyst support for Au nanoparticles (NPs) using the 4-nitrophenol reduction and the aldehyde-amine-alkyne (A3) coupling reactions to demonstrate its capabilities in regard to the ones of cellulose nanocrystals (CNCs)	1165:1398	Subsequently, we tested the ability of the as-made ChsNCs as a biomass-based catalyst support for Au nanoparticles (NPs) using the 4-nitrophenol reduction and the aldehyde-amine-alkyne (A3) coupling reactions to demonstrate its capabilities in regard to the ones of cellulose nanocrystals (CNCs).
32223230	7	46	theme	ChsNCs	1155:1160	arg1	ability					1132:1138	the ability	1128:1138	the ability of the as-made ChsNCs	1128:1160	Subsequently, we tested the ability of the as-made ChsNCs as a biomass-based catalyst support for Au nanoparticles (NPs) using the 4-nitrophenol reduction and the aldehyde-amine-alkyne (A3) coupling reactions to demonstrate its capabilities in regard to the ones of cellulose nanocrystals (CNCs).
32223230	3	47	theme	chitin	575:580	arg1	alcohols					593:600	chitin C6 primary alcohols	575:600	chitin C6 primary alcohols	575:600	Carboxylated chitin nanocrystals (ChNCs) were initially prepared through partial cleavage of glycosidic bonds in chitin by ammonium persulfate, with concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs.
32223230	5	48	theme	amino	869:873	arg1	groups					886:891	both carboxyl and amino functional groups	851:891	both carboxyl and amino functional groups	851:891	The resulting nanocrystals feature both carboxyl and amino functional groups.
32223230	1	49	theme	chitosan	201:208	arg1	ChsNC					224:228	ChsNC	224:228	ChsNC	224:228	In this study, we demonstrate for the first time the fabrication of carboxylated chitosan nanocrystals (ChsNC) with high degree of deacetylation (DDA) at >80% and narrow size distribution.
32223230	1	49	theme	chitosan	201:208	arg1	nanocrystals					210:221	carboxylated chitosan nanocrystals	188:221	carboxylated chitosan nanocrystals (ChsNC)	188:229	In this study, we demonstrate for the first time the fabrication of carboxylated chitosan nanocrystals (ChsNC) with high degree of deacetylation (DDA) at >80% and narrow size distribution.
32223230	3	50	theme	chitin	415:420	arg1	ChNCs					436:440	ChNCs	436:440	ChNCs	436:440	Carboxylated chitin nanocrystals (ChNCs) were initially prepared through partial cleavage of glycosidic bonds in chitin by ammonium persulfate, with concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs.
32223230	3	50	theme	chitin	415:420	arg1	nanocrystals					422:433	Carboxylated chitin nanocrystals	402:433	Carboxylated chitin nanocrystals (ChNCs)	402:441	Carboxylated chitin nanocrystals (ChNCs) were initially prepared through partial cleavage of glycosidic bonds in chitin by ammonium persulfate, with concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs.
32223230	9	51	theme	Au	1696:1697	arg1	state					1687:1691	the redox state	1677:1691	the redox state of Au as it is being deposited to afford a highly disperse active site on the bionano-support	1677:1785	Spectroscopic and imaging techniques confirmed the importance of precisely controlling the redox state of Au as it is being deposited to afford a highly disperse active site on the bionano-support.
32223230	3	52	theme	carboxylate	613:623	arg1	groups					625:630	carboxylate groups	613:630	carboxylate groups on the surface of the ChNCs	613:658	Carboxylated chitin nanocrystals (ChNCs) were initially prepared through partial cleavage of glycosidic bonds in chitin by ammonium persulfate, with concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs.
32223230	3	53	theme	C6	582:583	arg1	alcohols					593:600	chitin C6 primary alcohols	575:600	chitin C6 primary alcohols	575:600	Carboxylated chitin nanocrystals (ChNCs) were initially prepared through partial cleavage of glycosidic bonds in chitin by ammonium persulfate, with concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs.
32223230	1	54	theme	nanocrystals	210:221	arg1	fabrication					173:183	the fabrication	169:183	the fabrication of carboxylated chitosan nanocrystals (ChsNC) with high degree of deacetylation (DDA)	169:269	In this study, we demonstrate for the first time the fabrication of carboxylated chitosan nanocrystals (ChsNC) with high degree of deacetylation (DDA) at >80% and narrow size distribution.
32223230	5	55	theme	functional	875:884	arg1	groups					886:891	both carboxyl and amino functional groups	851:891	both carboxyl and amino functional groups	851:891	The resulting nanocrystals feature both carboxyl and amino functional groups.
32223230	8	56	theme	Au	1416:1417	arg1	NPs					1419:1421	Au NPs	1416:1421	Au NPs over ChsNCs	1416:1433	In particular, Au NPs over ChsNCs featured the highest turnover frequency (TOF) value for the 4-nitrophenol reduction reported for all Au-based catalysts supported on carbon-based systems.
32223230	8	57	theme	highest	1448:1454	arg1	value					1481:1485	the highest turnover frequency (TOF) value	1444:1485	the highest turnover frequency (TOF) value for the 4-nitrophenol reduction reported for all Au-based catalysts supported on carbon-based systems	1444:1587	In particular, Au NPs over ChsNCs featured the highest turnover frequency (TOF) value for the 4-nitrophenol reduction reported for all Au-based catalysts supported on carbon-based systems.
32223230	8	58	theme	turnover	1456:1463	arg1	value					1481:1485	the highest turnover frequency (TOF) value	1444:1485	the highest turnover frequency (TOF) value for the 4-nitrophenol reduction reported for all Au-based catalysts supported on carbon-based systems	1444:1587	In particular, Au NPs over ChsNCs featured the highest turnover frequency (TOF) value for the 4-nitrophenol reduction reported for all Au-based catalysts supported on carbon-based systems.
32223230	6	59	dep	infrared	981:988	arg1	FTIR					991:994	FTIR	991:994	FTIR	991:994	Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy were used to determine the morphology and composition of these carboxylated ChNCs and ChsNCs.
32223230	2	60	theme	support	358:364	arg1	material					366:373	a sustainable support material	344:373	a sustainable support material for metal-based catalysts	344:399	We also studied its application as a sustainable support material for metal-based catalysts.
32223230	2	60	theme	support	358:364	arg1	application					329:339	its application	325:339	its application	325:339	We also studied its application as a sustainable support material for metal-based catalysts.
32223230	1	61	with	fabrication	173:183	arg1	degree					241:246	high degree	236:246	high degree of deacetylation (DDA)	236:269	In this study, we demonstrate for the first time the fabrication of carboxylated chitosan nanocrystals (ChsNC) with high degree of deacetylation (DDA) at >80% and narrow size distribution.
32223230	4	62	theme	biomaterial	803:813	arg1	structure					786:794	the nanorod structure	774:794	the nanorod structure of the biomaterial	774:813	ChsNCs were subsequently prepared using an alkaline deacetylation procedure in the presence of NaBH4 to preserve the nanorod structure of the biomaterial.
32223230	3	63	theme	partial	475:481	arg1	cleavage					483:490	partial cleavage	475:490	partial cleavage of glycosidic bonds in chitin by ammonium persulfate	475:543	Carboxylated chitin nanocrystals (ChNCs) were initially prepared through partial cleavage of glycosidic bonds in chitin by ammonium persulfate, with concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs.
32223230	1	64	theme	narrow	283:288	arg1	distribution					295:306	narrow size distribution	283:306	narrow size distribution	283:306	In this study, we demonstrate for the first time the fabrication of carboxylated chitosan nanocrystals (ChsNC) with high degree of deacetylation (DDA) at >80% and narrow size distribution.
32223230	3	65	theme	ChNCs	654:658	arg1	surface					639:645	the surface	635:645	the surface of the ChNCs	635:658	Carboxylated chitin nanocrystals (ChNCs) were initially prepared through partial cleavage of glycosidic bonds in chitin by ammonium persulfate, with concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs.
32223230	9	66	theme	imaging	1608:1614	arg1	techniques					1616:1625	Spectroscopic and imaging techniques	1590:1625	techniques	1616:1625	Spectroscopic and imaging techniques confirmed the importance of precisely controlling the redox state of Au as it is being deposited to afford a highly disperse active site on the bionano-support.
32223230	4	67	theme	alkaline	704:711	arg1	procedure					727:735	an alkaline deacetylation procedure	701:735	an alkaline deacetylation procedure	701:735	ChsNCs were subsequently prepared using an alkaline deacetylation procedure in the presence of NaBH4 to preserve the nanorod structure of the biomaterial.
32223230	7	68	dep	catalyst	1181:1188	arg1	support					1190:1196	support	1190:1196	support for Au nanoparticles (NPs) using the 4-nitrophenol reduction and the aldehyde-amine-alkyne (A3) coupling reactions to demonstrate its capabilities in regard to the ones of cellulose nanocrystals (CNCs)	1190:1398	Subsequently, we tested the ability of the as-made ChsNCs as a biomass-based catalyst support for Au nanoparticles (NPs) using the 4-nitrophenol reduction and the aldehyde-amine-alkyne (A3) coupling reactions to demonstrate its capabilities in regard to the ones of cellulose nanocrystals (CNCs).
32223230	0	69	dep	Nanocrystals	22:33	arg1	Nanocrystals					22:33	Carboxylated Chitosan Nanocrystals	0:33	Carboxylated Chitosan Nanocrystals: A Synthetic Route and Application as Superior Support for Gold-Catalyzed Reactions.	0:118	Carboxylated Chitosan Nanocrystals: A Synthetic Route and Application as Superior Support for Gold-Catalyzed Reactions.
32223230	0	69	dep	Nanocrystals	22:33	arg1	Application					58:68	Application	58:68	Application as Superior Support for Gold-Catalyzed Reactions	58:117	Carboxylated Chitosan Nanocrystals: A Synthetic Route and Application as Superior Support for Gold-Catalyzed Reactions.
32223230	0	69	dep	Nanocrystals	22:33	arg1	Route					48:52	A Synthetic Route	36:52	A Synthetic Route	36:52	Carboxylated Chitosan Nanocrystals: A Synthetic Route and Application as Superior Support for Gold-Catalyzed Reactions.
32223230	6	70	theme	ChNCs	1086:1090	arg1	composition					1052:1062	composition	1052:1062	composition	1052:1062	Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy were used to determine the morphology and composition of these carboxylated ChNCs and ChsNCs.
32223230	6	70	theme	ChNCs	1086:1090	arg1	ChsNCs					1096:1101	ChsNCs	1096:1101	ChsNCs	1096:1101	Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy were used to determine the morphology and composition of these carboxylated ChNCs and ChsNCs.
32223230	6	70	theme	ChNCs	1086:1090	arg1	morphology					1037:1046	morphology	1037:1046	morphology	1037:1046	Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy were used to determine the morphology and composition of these carboxylated ChNCs and ChsNCs.
32223230	7	71	theme	Au	1202:1203	arg1	NPs					1220:1222	NPs	1220:1222	NPs	1220:1222	Subsequently, we tested the ability of the as-made ChsNCs as a biomass-based catalyst support for Au nanoparticles (NPs) using the 4-nitrophenol reduction and the aldehyde-amine-alkyne (A3) coupling reactions to demonstrate its capabilities in regard to the ones of cellulose nanocrystals (CNCs).
32223230	7	71	theme	Au	1202:1203	arg1	nanoparticles					1205:1217	Au nanoparticles	1202:1217	Au nanoparticles (NPs) using the 4-nitrophenol reduction and the aldehyde-amine-alkyne (A3) coupling reactions to demonstrate its capabilities in regard to the ones of cellulose nanocrystals (CNCs)	1202:1398	Subsequently, we tested the ability of the as-made ChsNCs as a biomass-based catalyst support for Au nanoparticles (NPs) using the 4-nitrophenol reduction and the aldehyde-amine-alkyne (A3) coupling reactions to demonstrate its capabilities in regard to the ones of cellulose nanocrystals (CNCs).
32223230	3	72	theme	primary	585:591	arg1	alcohols					593:600	chitin C6 primary alcohols	575:600	chitin C6 primary alcohols	575:600	Carboxylated chitin nanocrystals (ChNCs) were initially prepared through partial cleavage of glycosidic bonds in chitin by ammonium persulfate, with concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs.
32223230	3	73	theme	glycosidic	495:504	arg1	bonds					506:510	glycosidic bonds	495:510	glycosidic bonds in chitin	495:520	Carboxylated chitin nanocrystals (ChNCs) were initially prepared through partial cleavage of glycosidic bonds in chitin by ammonium persulfate, with concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs.
32223230	5	74	theme	carboxyl	856:863	arg1	groups					886:891	both carboxyl and amino functional groups	851:891	both carboxyl and amino functional groups	851:891	The resulting nanocrystals feature both carboxyl and amino functional groups.
32223230	9	75	dep	Au	1696:1697	arg1	deposited					1714:1722	deposited	1714:1722	is being deposited to afford a highly disperse active site on the bionano-support	1705:1785	Spectroscopic and imaging techniques confirmed the importance of precisely controlling the redox state of Au as it is being deposited to afford a highly disperse active site on the bionano-support.
32223230	1	76	theme	size	290:293	arg1	distribution					295:306	narrow size distribution	283:306	narrow size distribution	283:306	In this study, we demonstrate for the first time the fabrication of carboxylated chitosan nanocrystals (ChsNC) with high degree of deacetylation (DDA) at >80% and narrow size distribution.
32223230	3	77	theme	alcohols	593:600	arg1	oxidation					562:570	concurrent oxidation	551:570	concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs	551:658	Carboxylated chitin nanocrystals (ChNCs) were initially prepared through partial cleavage of glycosidic bonds in chitin by ammonium persulfate, with concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs.
32223230	3	78	theme	bonds	506:510	arg1	cleavage					483:490	partial cleavage	475:490	partial cleavage of glycosidic bonds in chitin by ammonium persulfate	475:543	Carboxylated chitin nanocrystals (ChNCs) were initially prepared through partial cleavage of glycosidic bonds in chitin by ammonium persulfate, with concurrent oxidation of chitin C6 primary alcohols to produce carboxylate groups on the surface of the ChNCs.
32223230	6	79	theme	X-ray	934:938	arg1	XRD					953:955	XRD	953:955	XRD	953:955	Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy were used to determine the morphology and composition of these carboxylated ChNCs and ChsNCs.
32223230	6	79	theme	X-ray	934:938	arg1	diffraction					940:950	X-ray diffraction	934:950	X-ray diffraction (XRD)	934:956	Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy were used to determine the morphology and composition of these carboxylated ChNCs and ChsNCs.
34364623	0	0	theme	resistant	89:97	arg1	properties					99:108	oxygen and water resistant properties	72:108	oxygen and water resistant properties	72:108	Preparation of bio-based cellulose acetate/chitosan composite film with oxygen and water resistant properties.
34364623	4	1	theme	hydrophobic	917:927	arg1	nature					929:934	the hydrophobic nature	913:934	the hydrophobic nature	913:934	The deposition of silica particles is able to compensate for the undesired increase in the hydrophilicity caused by the addition of chitosan, and tune the hydrophilic nature of the surface of the CA/CS films to the hydrophobic nature, which is desirable for water-resistant applications.
34364623	4	1	theme	hydrophobic	917:927	arg1	desirable					946:954	desirable	946:954	desirable	946:954	The deposition of silica particles is able to compensate for the undesired increase in the hydrophilicity caused by the addition of chitosan, and tune the hydrophilic nature of the surface of the CA/CS films to the hydrophobic nature, which is desirable for water-resistant applications.
34364623	1	2	theme	friendly	177:184	arg1	plastics					196:203	environmentally friendly bio-based plastics	161:203	environmentally friendly bio-based plastics that can replace petroleum-based plastics	161:245	Plastic pollution has inspired the preparation of environmentally friendly bio-based plastics that can replace petroleum-based plastics.
34364623	5	3	used	used	1081:1084	arg2	film					1013:1016	The prepared composite film	990:1016	The prepared composite film	990:1016	The prepared composite film displays good oxygen and water resistant properties and can be used for food packaging and related applications.
34364623	0	4	theme	water	83:87	arg1	properties					99:108	oxygen and water resistant properties	72:108	oxygen and water resistant properties	72:108	Preparation of bio-based cellulose acetate/chitosan composite film with oxygen and water resistant properties.
34364623	3	5	theme	membrane	649:656	arg1	stress					606:611	tensile stress	598:611	tensile stress	598:611	The addition of 15% chitosan greatly reduced the oxygen transmission rate of the CA film by 83.5%, and increased the tensile stress and tensile strain of the composite membrane, reaching 26.5 MPa and 22.2%, respectively.
34364623	3	5	theme	membrane	649:656	arg1	strain					625:630	tensile strain	617:630	tensile strain	617:630	The addition of 15% chitosan greatly reduced the oxygen transmission rate of the CA film by 83.5%, and increased the tensile stress and tensile strain of the composite membrane, reaching 26.5 MPa and 22.2%, respectively.
34364623	2	6	theme	cellulose	376:384	arg1	acetate					386:392	bio-based cellulose acetate	366:392	bio-based cellulose acetate (CA)	366:397	Herein, a composite film with oxygen and water resistant properties was prepared by a fluidized bed method, employing bio-based cellulose acetate (CA) as raw material, glycerol as a plasticizer, and chitosan and silica as additives.
34364623	2	6	theme	cellulose	376:384	arg1	CA					395:396	CA	395:396	CA	395:396	Herein, a composite film with oxygen and water resistant properties was prepared by a fluidized bed method, employing bio-based cellulose acetate (CA) as raw material, glycerol as a plasticizer, and chitosan and silica as additives.
34364623	4	7	theme	chitosan	834:841	arg1	addition					822:829	the addition	818:829	the addition of chitosan	818:841	The deposition of silica particles is able to compensate for the undesired increase in the hydrophilicity caused by the addition of chitosan, and tune the hydrophilic nature of the surface of the CA/CS films to the hydrophobic nature, which is desirable for water-resistant applications.
34364623	3	8	theme	oxygen	530:535	arg1	rate					550:553	the oxygen transmission rate	526:553	the oxygen transmission rate of the CA film	526:568	The addition of 15% chitosan greatly reduced the oxygen transmission rate of the CA film by 83.5%, and increased the tensile stress and tensile strain of the composite membrane, reaching 26.5 MPa and 22.2%, respectively.
34364623	4	9	theme	surface	883:889	arg1	nature					869:874	the hydrophilic nature	853:874	the hydrophilic nature of the surface of the CA/CS films to the hydrophobic nature, which is desirable for water-resistant applications	853:987	The deposition of silica particles is able to compensate for the undesired increase in the hydrophilicity caused by the addition of chitosan, and tune the hydrophilic nature of the surface of the CA/CS films to the hydrophobic nature, which is desirable for water-resistant applications.
34364623	3	10	theme	transmission	537:548	arg1	rate					550:553	the oxygen transmission rate	526:553	the oxygen transmission rate of the CA film	526:568	The addition of 15% chitosan greatly reduced the oxygen transmission rate of the CA film by 83.5%, and increased the tensile stress and tensile strain of the composite membrane, reaching 26.5 MPa and 22.2%, respectively.
34364623	4	11	theme	CA/CS	898:902	arg1	films					904:908	the CA/CS films	894:908	the CA/CS films	894:908	The deposition of silica particles is able to compensate for the undesired increase in the hydrophilicity caused by the addition of chitosan, and tune the hydrophilic nature of the surface of the CA/CS films to the hydrophobic nature, which is desirable for water-resistant applications.
34364623	5	12	theme	water	1043:1047	arg1	properties					1059:1068	good oxygen and water resistant properties	1027:1068	properties	1059:1068	The prepared composite film displays good oxygen and water resistant properties and can be used for food packaging and related applications.
34364623	1	13	theme	plastics	196:203	arg1	preparation					146:156	the preparation	142:156	the preparation of environmentally friendly bio-based plastics that can replace petroleum-based plastics	142:245	Plastic pollution has inspired the preparation of environmentally friendly bio-based plastics that can replace petroleum-based plastics.
34364623	5	14	theme	resistant	1049:1057	arg1	properties					1059:1068	good oxygen and water resistant properties	1027:1068	properties	1059:1068	The prepared composite film displays good oxygen and water resistant properties and can be used for food packaging and related applications.
34364623	2	15	theme	resistant	295:303	arg1	properties					305:314	oxygen and water resistant properties	278:314	oxygen and water resistant properties	278:314	Herein, a composite film with oxygen and water resistant properties was prepared by a fluidized bed method, employing bio-based cellulose acetate (CA) as raw material, glycerol as a plasticizer, and chitosan and silica as additives.
34364623	2	16	theme	raw	402:404	arg1	material					406:413	raw material	402:413	raw material	402:413	Herein, a composite film with oxygen and water resistant properties was prepared by a fluidized bed method, employing bio-based cellulose acetate (CA) as raw material, glycerol as a plasticizer, and chitosan and silica as additives.
34364623	2	17	theme	bed	344:346	arg1	method					348:353	a fluidized bed method	332:353	a fluidized bed method	332:353	Herein, a composite film with oxygen and water resistant properties was prepared by a fluidized bed method, employing bio-based cellulose acetate (CA) as raw material, glycerol as a plasticizer, and chitosan and silica as additives.
34364623	5	18	theme	related	1109:1115	arg1	applications					1117:1128	related applications	1109:1128	related applications	1109:1128	The prepared composite film displays good oxygen and water resistant properties and can be used for food packaging and related applications.
34364623	2	19	theme	fluidized	334:342	arg1	method					348:353	a fluidized bed method	332:353	a fluidized bed method	332:353	Herein, a composite film with oxygen and water resistant properties was prepared by a fluidized bed method, employing bio-based cellulose acetate (CA) as raw material, glycerol as a plasticizer, and chitosan and silica as additives.
34364623	0	20	theme	cellulose	25:33	arg1	Preparation					0:10	Preparation	0:10	Preparation of bio-based cellulose	0:33	Preparation of bio-based cellulose acetate/chitosan composite film with oxygen and water resistant properties.
34364623	1	21	theme	Plastic	111:117	arg1	pollution					119:127	Plastic pollution	111:127	Plastic pollution	111:127	Plastic pollution has inspired the preparation of environmentally friendly bio-based plastics that can replace petroleum-based plastics.
34364623	1	22	theme	petroleum-based	222:236	arg1	plastics					238:245	petroleum-based plastics	222:245	petroleum-based plastics	222:245	Plastic pollution has inspired the preparation of environmentally friendly bio-based plastics that can replace petroleum-based plastics.
34364623	0	23	theme	bio-based	15:23	arg1	cellulose					25:33	bio-based cellulose	15:33	bio-based cellulose	15:33	Preparation of bio-based cellulose acetate/chitosan composite film with oxygen and water resistant properties.
34364623	2	24	with	film	268:271	arg1	properties					305:314	oxygen and water resistant properties	278:314	oxygen and water resistant properties	278:314	Herein, a composite film with oxygen and water resistant properties was prepared by a fluidized bed method, employing bio-based cellulose acetate (CA) as raw material, glycerol as a plasticizer, and chitosan and silica as additives.
34364623	4	25	theme	hydrophilic	857:867	arg1	nature					869:874	the hydrophilic nature	853:874	the hydrophilic nature of the surface of the CA/CS films to the hydrophobic nature, which is desirable for water-resistant applications	853:987	The deposition of silica particles is able to compensate for the undesired increase in the hydrophilicity caused by the addition of chitosan, and tune the hydrophilic nature of the surface of the CA/CS films to the hydrophobic nature, which is desirable for water-resistant applications.
34364623	1	26	theme	bio-based	186:194	arg1	plastics					196:203	environmentally friendly bio-based plastics	161:203	environmentally friendly bio-based plastics that can replace petroleum-based plastics	161:245	Plastic pollution has inspired the preparation of environmentally friendly bio-based plastics that can replace petroleum-based plastics.
34364623	0	27	theme	composite	52:60	arg1	film					62:65	composite film	52:65	composite film with oxygen and water resistant properties	52:108	Preparation of bio-based cellulose acetate/chitosan composite film with oxygen and water resistant properties.
34364623	3	28	theme	tensile	617:623	arg1	strain					625:630	tensile strain	617:630	tensile strain	617:630	The addition of 15% chitosan greatly reduced the oxygen transmission rate of the CA film by 83.5%, and increased the tensile stress and tensile strain of the composite membrane, reaching 26.5 MPa and 22.2%, respectively.
34364623	4	29	theme	undesired	767:775	arg1	increase					777:784	the undesired increase	763:784	the undesired increase in the hydrophilicity caused by the addition of chitosan	763:841	The deposition of silica particles is able to compensate for the undesired increase in the hydrophilicity caused by the addition of chitosan, and tune the hydrophilic nature of the surface of the CA/CS films to the hydrophobic nature, which is desirable for water-resistant applications.
34364623	3	30	theme	CA	562:563	arg1	film					565:568	the CA film	558:568	the CA film	558:568	The addition of 15% chitosan greatly reduced the oxygen transmission rate of the CA film by 83.5%, and increased the tensile stress and tensile strain of the composite membrane, reaching 26.5 MPa and 22.2%, respectively.
34364623	3	31	theme	tensile	598:604	arg1	stress					606:611	tensile stress	598:611	tensile stress	598:611	The addition of 15% chitosan greatly reduced the oxygen transmission rate of the CA film by 83.5%, and increased the tensile stress and tensile strain of the composite membrane, reaching 26.5 MPa and 22.2%, respectively.
34364623	2	32	theme	composite	258:266	arg1	film					268:271	a composite film	256:271	a composite film with oxygen and water resistant properties	256:314	Herein, a composite film with oxygen and water resistant properties was prepared by a fluidized bed method, employing bio-based cellulose acetate (CA) as raw material, glycerol as a plasticizer, and chitosan and silica as additives.
34364623	3	33	theme	15	497:498	arg1	%					499:499	%	499:499	%	499:499	The addition of 15% chitosan greatly reduced the oxygen transmission rate of the CA film by 83.5%, and increased the tensile stress and tensile strain of the composite membrane, reaching 26.5 MPa and 22.2%, respectively.
34364623	4	34	theme	films	904:908	arg1	surface					883:889	the surface	879:889	the surface of the CA/CS films	879:908	The deposition of silica particles is able to compensate for the undesired increase in the hydrophilicity caused by the addition of chitosan, and tune the hydrophilic nature of the surface of the CA/CS films to the hydrophobic nature, which is desirable for water-resistant applications.
34364623	3	35	theme	film	565:568	arg1	rate					550:553	the oxygen transmission rate	526:553	the oxygen transmission rate of the CA film	526:568	The addition of 15% chitosan greatly reduced the oxygen transmission rate of the CA film by 83.5%, and increased the tensile stress and tensile strain of the composite membrane, reaching 26.5 MPa and 22.2%, respectively.
34364623	4	36	from	increase	777:784	arg1	hydrophilicity					793:806	the hydrophilicity	789:806	the hydrophilicity caused by the addition of chitosan	789:841	The deposition of silica particles is able to compensate for the undesired increase in the hydrophilicity caused by the addition of chitosan, and tune the hydrophilic nature of the surface of the CA/CS films to the hydrophobic nature, which is desirable for water-resistant applications.
34364623	2	37	theme	bio-based	366:374	arg1	acetate					386:392	bio-based cellulose acetate	366:392	bio-based cellulose acetate (CA)	366:397	Herein, a composite film with oxygen and water resistant properties was prepared by a fluidized bed method, employing bio-based cellulose acetate (CA) as raw material, glycerol as a plasticizer, and chitosan and silica as additives.
34364623	2	37	theme	bio-based	366:374	arg1	CA					395:396	CA	395:396	CA	395:396	Herein, a composite film with oxygen and water resistant properties was prepared by a fluidized bed method, employing bio-based cellulose acetate (CA) as raw material, glycerol as a plasticizer, and chitosan and silica as additives.
34364623	3	38	theme	%	499:499	arg1	chitosan					501:508	15% chitosan	497:508	15% chitosan	497:508	The addition of 15% chitosan greatly reduced the oxygen transmission rate of the CA film by 83.5%, and increased the tensile stress and tensile strain of the composite membrane, reaching 26.5 MPa and 22.2%, respectively.
34364623	5	39	theme	good	1027:1030	arg1	oxygen					1032:1037	good oxygen and water resistant properties	1027:1068	oxygen	1032:1037	The prepared composite film displays good oxygen and water resistant properties and can be used for food packaging and related applications.
34364623	3	40	theme	composite	639:647	arg1	membrane					649:656	the composite membrane	635:656	the composite membrane	635:656	The addition of 15% chitosan greatly reduced the oxygen transmission rate of the CA film by 83.5%, and increased the tensile stress and tensile strain of the composite membrane, reaching 26.5 MPa and 22.2%, respectively.
34364623	2	41	theme	water	289:293	arg1	properties					305:314	oxygen and water resistant properties	278:314	oxygen and water resistant properties	278:314	Herein, a composite film with oxygen and water resistant properties was prepared by a fluidized bed method, employing bio-based cellulose acetate (CA) as raw material, glycerol as a plasticizer, and chitosan and silica as additives.
34364623	4	42	theme	particles	727:735	arg1	able					740:743	able	740:743	able	740:743	The deposition of silica particles is able to compensate for the undesired increase in the hydrophilicity caused by the addition of chitosan, and tune the hydrophilic nature of the surface of the CA/CS films to the hydrophobic nature, which is desirable for water-resistant applications.
34364623	4	42	theme	particles	727:735	arg1	deposition					706:715	The deposition	702:715	The deposition of silica particles	702:735	The deposition of silica particles is able to compensate for the undesired increase in the hydrophilicity caused by the addition of chitosan, and tune the hydrophilic nature of the surface of the CA/CS films to the hydrophobic nature, which is desirable for water-resistant applications.
34364623	5	43	theme	prepared	994:1001	arg1	film					1013:1016	The prepared composite film	990:1016	The prepared composite film	990:1016	The prepared composite film displays good oxygen and water resistant properties and can be used for food packaging and related applications.
34364623	4	44	theme	silica	720:725	arg1	particles					727:735	silica particles	720:735	silica particles	720:735	The deposition of silica particles is able to compensate for the undesired increase in the hydrophilicity caused by the addition of chitosan, and tune the hydrophilic nature of the surface of the CA/CS films to the hydrophobic nature, which is desirable for water-resistant applications.
34364623	3	45	dep	stress	606:611	arg1	the					594:596	the	594:596	the	594:596	The addition of 15% chitosan greatly reduced the oxygen transmission rate of the CA film by 83.5%, and increased the tensile stress and tensile strain of the composite membrane, reaching 26.5 MPa and 22.2%, respectively.
34364623	5	46	theme	composite	1003:1011	arg1	film					1013:1016	The prepared composite film	990:1016	The prepared composite film	990:1016	The prepared composite film displays good oxygen and water resistant properties and can be used for food packaging and related applications.
34364623	2	47	theme	oxygen	278:283	arg1	properties					305:314	oxygen and water resistant properties	278:314	oxygen and water resistant properties	278:314	Herein, a composite film with oxygen and water resistant properties was prepared by a fluidized bed method, employing bio-based cellulose acetate (CA) as raw material, glycerol as a plasticizer, and chitosan and silica as additives.
34364623	0	48	with	film	62:65	arg1	properties					99:108	oxygen and water resistant properties	72:108	oxygen and water resistant properties	72:108	Preparation of bio-based cellulose acetate/chitosan composite film with oxygen and water resistant properties.
34364623	3	49	theme	chitosan	501:508	arg1	addition					485:492	The addition	481:492	The addition of 15% chitosan	481:508	The addition of 15% chitosan greatly reduced the oxygen transmission rate of the CA film by 83.5%, and increased the tensile stress and tensile strain of the composite membrane, reaching 26.5 MPa and 22.2%, respectively.
34364623	0	50	theme	oxygen	72:77	arg1	properties					99:108	oxygen and water resistant properties	72:108	oxygen and water resistant properties	72:108	Preparation of bio-based cellulose acetate/chitosan composite film with oxygen and water resistant properties.
34364623	4	51	theme	water-resistant	960:974	arg1	applications					976:987	water-resistant applications	960:987	water-resistant applications	960:987	The deposition of silica particles is able to compensate for the undesired increase in the hydrophilicity caused by the addition of chitosan, and tune the hydrophilic nature of the surface of the CA/CS films to the hydrophobic nature, which is desirable for water-resistant applications.
34364623	1	52	theme	environmentally	161:175	arg1	plastics					196:203	environmentally friendly bio-based plastics	161:203	environmentally friendly bio-based plastics that can replace petroleum-based plastics	161:245	Plastic pollution has inspired the preparation of environmentally friendly bio-based plastics that can replace petroleum-based plastics.
34364623	5	53	theme	food	1090:1093	arg1	packaging					1095:1103	food packaging	1090:1103	food packaging	1090:1103	The prepared composite film displays good oxygen and water resistant properties and can be used for food packaging and related applications.
33593565	5	0	theme	phlorotannin	628:639	arg1	content					641:647	the phlorotannin content	624:647	the phlorotannin content	624:647	By an in vitro experimental approach in chondrocytes and fibroblast-like synoviocytes, we observed that chemical composition of fucoidan, and specifically the phlorotannin content and the ratio sulfate:fucose, seems critically relevant for its biological activity.
33593565	5	1	theme	chemical	573:580	arg1	composition					582:592	chemical composition	573:592	chemical composition of fucoidan	573:604	By an in vitro experimental approach in chondrocytes and fibroblast-like synoviocytes, we observed that chemical composition of fucoidan, and specifically the phlorotannin content and the ratio sulfate:fucose, seems critically relevant for its biological activity.
33593565	0	2	from	applications	61:72	arg1	osteoarthritis					77:90	osteoarthritis	77:90	osteoarthritis	77:90	Study of fucoidans as natural biomolecules for therapeutical applications in osteoarthritis.
33593565	8	3	theme	pathological	1030:1041	arg1	processes					1043:1051	key pathological processes	1026:1051	key pathological processes in the joint that favor the development of rheumatic disorders as OA	1026:1120	Thus, our results shed light on the potential use of fucoidans as natural molecules in the treatment of key pathological processes in the joint that favor the development of rheumatic disorders as OA.
33593565	4	4	theme	specific	436:443	arg1	processes					458:466	specific pathological processes	436:466	specific pathological processes	436:466	However, the variability between fucoidans makes difficult the pursuit of the most suitable candidate to target specific pathological processes.
33593565	5	5	theme	biological	713:722	arg1	activity					724:731	its biological activity	709:731	its biological activity	709:731	By an in vitro experimental approach in chondrocytes and fibroblast-like synoviocytes, we observed that chemical composition of fucoidan, and specifically the phlorotannin content and the ratio sulfate:fucose, seems critically relevant for its biological activity.
33593565	8	6	theme	key	1026:1028	arg1	processes					1043:1051	key pathological processes	1026:1051	key pathological processes in the joint that favor the development of rheumatic disorders as OA	1026:1120	Thus, our results shed light on the potential use of fucoidans as natural molecules in the treatment of key pathological processes in the joint that favor the development of rheumatic disorders as OA.
33593565	5	7	theme	fucoidan	597:604	arg1	composition					582:592	chemical composition	573:592	chemical composition of fucoidan	573:604	By an in vitro experimental approach in chondrocytes and fibroblast-like synoviocytes, we observed that chemical composition of fucoidan, and specifically the phlorotannin content and the ratio sulfate:fucose, seems critically relevant for its biological activity.
33593565	5	7	theme	fucoidan	597:604	arg1	sulfate					663:669	the ratio sulfate	653:669	the ratio sulfate	653:669	By an in vitro experimental approach in chondrocytes and fibroblast-like synoviocytes, we observed that chemical composition of fucoidan, and specifically the phlorotannin content and the ratio sulfate:fucose, seems critically relevant for its biological activity.
33593565	5	7	theme	fucoidan	597:604	arg1	content					641:647	the phlorotannin content	624:647	the phlorotannin content	624:647	By an in vitro experimental approach in chondrocytes and fibroblast-like synoviocytes, we observed that chemical composition of fucoidan, and specifically the phlorotannin content and the ratio sulfate:fucose, seems critically relevant for its biological activity.
33593565	5	8	from	approach	497:504	arg1	chondrocytes					509:520	chondrocytes	509:520	chondrocytes	509:520	By an in vitro experimental approach in chondrocytes and fibroblast-like synoviocytes, we observed that chemical composition of fucoidan, and specifically the phlorotannin content and the ratio sulfate:fucose, seems critically relevant for its biological activity.
33593565	5	8	from	approach	497:504	arg1	synoviocytes					542:553	fibroblast-like synoviocytes	526:553	fibroblast-like synoviocytes	526:553	By an in vitro experimental approach in chondrocytes and fibroblast-like synoviocytes, we observed that chemical composition of fucoidan, and specifically the phlorotannin content and the ratio sulfate:fucose, seems critically relevant for its biological activity.
33593565	3	9	theme	OA	310:311	arg1	treatment					313:321	OA treatment	310:321	OA treatment	310:321	Fucoidans, one of the main therapeutic components of brown algae, have emerged as promising molecules in OA treatment.
33593565	8	10	theme	fucoidans	975:983	arg1	use					968:970	the potential use	954:970	the potential use of fucoidans as natural molecules in the treatment of key pathological processes in the joint that favor the development of rheumatic disorders as OA	954:1120	Thus, our results shed light on the potential use of fucoidans as natural molecules in the treatment of key pathological processes in the joint that favor the development of rheumatic disorders as OA.
33593565	4	11	theme	pathological	445:456	arg1	processes					458:466	specific pathological processes	436:466	specific pathological processes	436:466	However, the variability between fucoidans makes difficult the pursuit of the most suitable candidate to target specific pathological processes.
33593565	8	12	theme	processes	1043:1051	arg1	treatment					1013:1021	the treatment	1009:1021	the treatment of key pathological processes in the joint that favor the development of rheumatic disorders as OA	1009:1120	Thus, our results shed light on the potential use of fucoidans as natural molecules in the treatment of key pathological processes in the joint that favor the development of rheumatic disorders as OA.
33593565	8	13	theme	disorders	1106:1114	arg1	development					1081:1091	the development	1077:1091	the development of rheumatic disorders as OA	1077:1120	Thus, our results shed light on the potential use of fucoidans as natural molecules in the treatment of key pathological processes in the joint that favor the development of rheumatic disorders as OA.
33593565	5	14	theme	in	475:476	arg1	approach					497:504	an in vitro experimental approach	472:504	an in vitro experimental approach in chondrocytes and fibroblast-like synoviocytes	472:553	By an in vitro experimental approach in chondrocytes and fibroblast-like synoviocytes, we observed that chemical composition of fucoidan, and specifically the phlorotannin content and the ratio sulfate:fucose, seems critically relevant for its biological activity.
33593565	8	15	from	treatment	1013:1021	arg1	joint					1060:1064	the joint	1056:1064	the joint that favor the development of rheumatic disorders as OA	1056:1120	Thus, our results shed light on the potential use of fucoidans as natural molecules in the treatment of key pathological processes in the joint that favor the development of rheumatic disorders as OA.
33593565	6	16	theme	fucoidan	808:815	arg1	concentration					766:778	concentration	766:778	concentration	766:778	Nonetheless, other factors like concentration and molecular weight of the fucoidan may influence on its beneficial effects.
33593565	6	16	theme	fucoidan	808:815	arg1	weight					794:799	molecular weight	784:799	molecular weight	784:799	Nonetheless, other factors like concentration and molecular weight of the fucoidan may influence on its beneficial effects.
33593565	8	17	theme	natural	988:994	arg1	molecules					996:1004	natural molecules	988:1004	natural molecules in the treatment of key pathological processes in the joint that favor the development of rheumatic disorders as OA	988:1120	Thus, our results shed light on the potential use of fucoidans as natural molecules in the treatment of key pathological processes in the joint that favor the development of rheumatic disorders as OA.
33593565	3	18	theme	components	244:253	arg1	components					244:253	the main therapeutic components	223:253	the main therapeutic components of brown algae	223:268	Fucoidans, one of the main therapeutic components of brown algae, have emerged as promising molecules in OA treatment.
33593565	3	18	theme	components	244:253	arg1	one					216:218	one	216:218	one	216:218	Fucoidans, one of the main therapeutic components of brown algae, have emerged as promising molecules in OA treatment.
33593565	3	18	theme	components	244:253	arg1	Fucoidans					205:213	Fucoidans	205:213	Fucoidans	205:213	Fucoidans, one of the main therapeutic components of brown algae, have emerged as promising molecules in OA treatment.
33593565	6	19	theme	molecular	784:792	arg1	weight					794:799	molecular weight	784:799	molecular weight	784:799	Nonetheless, other factors like concentration and molecular weight of the fucoidan may influence on its beneficial effects.
33593565	0	20	theme	fucoidans	9:17	arg1	Study					0:4	Study	0:4	Study of fucoidans as natural biomolecules for therapeutical applications in osteoarthritis.	0:91	Study of fucoidans as natural biomolecules for therapeutical applications in osteoarthritis.
33593565	4	21	theme	suitable	407:414	arg1	candidate					416:424	the most suitable candidate	398:424	the most suitable candidate	398:424	However, the variability between fucoidans makes difficult the pursuit of the most suitable candidate to target specific pathological processes.
33593565	8	22	from	molecules	996:1004	arg1	treatment					1013:1021	the treatment	1009:1021	the treatment of key pathological processes in the joint that favor the development of rheumatic disorders as OA	1009:1120	Thus, our results shed light on the potential use of fucoidans as natural molecules in the treatment of key pathological processes in the joint that favor the development of rheumatic disorders as OA.
33593565	3	23	theme	main	227:230	arg1	components					244:253	the main therapeutic components	223:253	the main therapeutic components of brown algae	223:268	Fucoidans, one of the main therapeutic components of brown algae, have emerged as promising molecules in OA treatment.
33593565	0	24	theme	natural	22:28	arg1	biomolecules					30:41	natural biomolecules	22:41	natural biomolecules for therapeutical applications in osteoarthritis	22:90	Study of fucoidans as natural biomolecules for therapeutical applications in osteoarthritis.
33593565	8	25	from	processes	1043:1051	arg1	joint					1060:1064	the joint	1056:1064	the joint that favor the development of rheumatic disorders as OA	1056:1120	Thus, our results shed light on the potential use of fucoidans as natural molecules in the treatment of key pathological processes in the joint that favor the development of rheumatic disorders as OA.
33593565	3	26	theme	therapeutic	232:242	arg1	components					244:253	the main therapeutic components	223:253	the main therapeutic components of brown algae	223:268	Fucoidans, one of the main therapeutic components of brown algae, have emerged as promising molecules in OA treatment.
33593565	6	27	theme	beneficial	838:847	arg1	effects					849:855	its beneficial effects	834:855	its beneficial effects	834:855	Nonetheless, other factors like concentration and molecular weight of the fucoidan may influence on its beneficial effects.
33593565	6	28	theme	other	747:751	arg1	factors					753:759	other factors	747:759	other factors like concentration and molecular weight of the fucoidan	747:815	Nonetheless, other factors like concentration and molecular weight of the fucoidan may influence on its beneficial effects.
33593565	0	29	theme	therapeutical	47:59	arg1	applications					61:72	therapeutical applications	47:72	therapeutical applications in osteoarthritis	47:90	Study of fucoidans as natural biomolecules for therapeutical applications in osteoarthritis.
33593565	5	30	theme	ratio	657:661	arg1	sulfate					663:669	the ratio sulfate	653:669	the ratio sulfate	653:669	By an in vitro experimental approach in chondrocytes and fibroblast-like synoviocytes, we observed that chemical composition of fucoidan, and specifically the phlorotannin content and the ratio sulfate:fucose, seems critically relevant for its biological activity.
33593565	4	31	theme	candidate	416:424	arg1	pursuit					387:393	the pursuit	383:393	the pursuit of the most suitable candidate	383:424	However, the variability between fucoidans makes difficult the pursuit of the most suitable candidate to target specific pathological processes.
33593565	5	32	dep	in	475:476	arg1	vitro					478:482	vitro	478:482	vitro	478:482	By an in vitro experimental approach in chondrocytes and fibroblast-like synoviocytes, we observed that chemical composition of fucoidan, and specifically the phlorotannin content and the ratio sulfate:fucose, seems critically relevant for its biological activity.
33593565	5	33	theme	fibroblast-like	526:540	arg1	synoviocytes					542:553	fibroblast-like synoviocytes	526:553	fibroblast-like synoviocytes	526:553	By an in vitro experimental approach in chondrocytes and fibroblast-like synoviocytes, we observed that chemical composition of fucoidan, and specifically the phlorotannin content and the ratio sulfate:fucose, seems critically relevant for its biological activity.
33593565	3	34	theme	brown	258:262	arg1	algae					264:268	brown algae	258:268	brown algae	258:268	Fucoidans, one of the main therapeutic components of brown algae, have emerged as promising molecules in OA treatment.
33593565	8	35	theme	potential	958:966	arg1	use					968:970	the potential use	954:970	the potential use of fucoidans as natural molecules in the treatment of key pathological processes in the joint that favor the development of rheumatic disorders as OA	954:1120	Thus, our results shed light on the potential use of fucoidans as natural molecules in the treatment of key pathological processes in the joint that favor the development of rheumatic disorders as OA.
33593565	3	36	theme	algae	264:268	arg1	components					244:253	the main therapeutic components	223:253	the main therapeutic components of brown algae	223:268	Fucoidans, one of the main therapeutic components of brown algae, have emerged as promising molecules in OA treatment.
33593565	1	37	theme	prevalent	125:133	arg1	disease					153:159	the most prevalent articular chronic disease	116:159	the most prevalent articular chronic disease	116:159	Osteoarthritis (OA) is the most prevalent articular chronic disease.
33593565	1	37	theme	prevalent	125:133	arg1	Osteoarthritis					93:106	Osteoarthritis	93:106	Osteoarthritis (OA)	93:111	Osteoarthritis (OA) is the most prevalent articular chronic disease.
33593565	7	38	theme	dependent	884:892	arg1	response					894:901	a cell-type dependent response	872:901	a cell-type dependent response	872:901	Additionally, a cell-type dependent response was also detected.
33593565	8	39	from	joint	1060:1064	arg1	treatment					1013:1021	the treatment	1009:1021	the treatment of key pathological processes in the joint that favor the development of rheumatic disorders as OA	1009:1120	Thus, our results shed light on the potential use of fucoidans as natural molecules in the treatment of key pathological processes in the joint that favor the development of rheumatic disorders as OA.
33593565	3	40	theme	promising	287:295	arg1	molecules					297:305	promising molecules	287:305	promising molecules in OA treatment	287:321	Fucoidans, one of the main therapeutic components of brown algae, have emerged as promising molecules in OA treatment.
33593565	3	41	from	molecules	297:305	arg1	treatment					313:321	OA treatment	310:321	OA treatment	310:321	Fucoidans, one of the main therapeutic components of brown algae, have emerged as promising molecules in OA treatment.
33593565	5	42	theme	experimental	484:495	arg1	approach					497:504	an in vitro experimental approach	472:504	an in vitro experimental approach in chondrocytes and fibroblast-like synoviocytes	472:553	By an in vitro experimental approach in chondrocytes and fibroblast-like synoviocytes, we observed that chemical composition of fucoidan, and specifically the phlorotannin content and the ratio sulfate:fucose, seems critically relevant for its biological activity.
33593565	1	43	theme	articular	135:143	arg1	disease					153:159	the most prevalent articular chronic disease	116:159	the most prevalent articular chronic disease	116:159	Osteoarthritis (OA) is the most prevalent articular chronic disease.
33593565	1	43	theme	articular	135:143	arg1	Osteoarthritis					93:106	Osteoarthritis	93:106	Osteoarthritis (OA)	93:111	Osteoarthritis (OA) is the most prevalent articular chronic disease.
33593565	8	44	theme	rheumatic	1096:1104	arg1	disorders					1106:1114	rheumatic disorders	1096:1114	rheumatic disorders as OA	1096:1120	Thus, our results shed light on the potential use of fucoidans as natural molecules in the treatment of key pathological processes in the joint that favor the development of rheumatic disorders as OA.
33593565	5	45	dep	content	641:647	arg1	fucose					671:676	fucose	671:676	the phlorotannin content and the ratio sulfate:fucose	624:676	By an in vitro experimental approach in chondrocytes and fibroblast-like synoviocytes, we observed that chemical composition of fucoidan, and specifically the phlorotannin content and the ratio sulfate:fucose, seems critically relevant for its biological activity.
33593565	1	46	theme	chronic	145:151	arg1	disease					153:159	the most prevalent articular chronic disease	116:159	the most prevalent articular chronic disease	116:159	Osteoarthritis (OA) is the most prevalent articular chronic disease.
33593565	1	46	theme	chronic	145:151	arg1	Osteoarthritis					93:106	Osteoarthritis	93:106	Osteoarthritis (OA)	93:111	Osteoarthritis (OA) is the most prevalent articular chronic disease.
33593565	4	47	dep	difficult	373:381	arg1	target					429:434	target	429:434	to target specific pathological processes	426:466	However, the variability between fucoidans makes difficult the pursuit of the most suitable candidate to target specific pathological processes.
31935863	7	0	theme	2.02	1203:1206	arg1	mmol					1208:1211	mmol	1208:1211	mmol	1208:1211	The chitosan-based CH-AN bio-composite exhibited greater sorption capacity for lead (2.54 mmol g-1) and copper (2.02 mmol g-1) than precursor chitosan from aqueous solution based on Langmuir sorption isotherm.
31935863	10	1	theme	nonlinear	1534:1542	arg1	analysis					1555:1562	The nonlinear regression analysis	1530:1562	The nonlinear regression analysis	1530:1562	The nonlinear regression analysis showed lower values of error functions as compared with linear regression analysis.
31935863	7	2	theme	chitosan-based	1095:1108	arg1	bio-composite					1116:1128	The chitosan-based CH-AN bio-composite	1091:1128	The chitosan-based CH-AN bio-composite	1091:1128	The chitosan-based CH-AN bio-composite exhibited greater sorption capacity for lead (2.54 mmol g-1) and copper (2.02 mmol g-1) than precursor chitosan from aqueous solution based on Langmuir sorption isotherm.
31935863	2	3	theme	current	264:270	arg1	work					272:275	The current work	260:275	The current work	260:275	The current work aimed to increase the Lewis basic centers on the polymeric backbone using single-step three-components (chitosan, carbon disulfide, and acrylonitrile) reaction.
31935863	1	4	theme	disulfide	249:257	arg1	presence					230:237	the presence	226:237	the presence of carbon disulfide	226:257	Herein, we report the development of chitosan (CH)-based bio-composite modified with acrylonitrile (AN) in the presence of carbon disulfide.
31935863	4	5	theme	electron	823:830	arg1	microscopy					832:841	scanning electron microscopy	814:841	scanning electron microscopy (SEM)	814:847	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	4	5	theme	electron	823:830	arg1	SEM					844:846	SEM	844:846	SEM	844:846	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	2	6	from	centers	311:317	arg1	backbone					336:343	the polymeric backbone	322:343	the polymeric backbone using single-step three-components (chitosan, carbon disulfide, and acrylonitrile) reaction	322:435	The current work aimed to increase the Lewis basic centers on the polymeric backbone using single-step three-components (chitosan, carbon disulfide, and acrylonitrile) reaction.
31935863	10	7	theme	lower	1571:1575	arg1	values					1577:1582	lower values	1571:1582	lower values of error functions	1571:1601	The nonlinear regression analysis showed lower values of error functions as compared with linear regression analysis.
31935863	3	8	theme	pendant	503:509	arg1	NH2					529:531	NH2	529:531	NH2	529:531	For a said purpose, the thiocarbamate moiety was attached to the pendant functional amine (NH2) of chitosan.
31935863	3	8	theme	pendant	503:509	arg1	amine					522:526	the pendant functional amine	499:526	the pendant functional amine (NH2) of chitosan	499:544	For a said purpose, the thiocarbamate moiety was attached to the pendant functional amine (NH2) of chitosan.
31935863	4	9	theme	analytical	642:651	arg1	investigation					767:779	elemental investigation	757:779	elemental investigation	757:779	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	4	9	theme	analytical	642:651	arg1	13C-NMR					687:693	13C-NMR	687:693	13C-NMR (solid-form)	687:706	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	4	9	theme	analytical	642:651	arg1	spectroscopy					736:747	Fourier-transform infrared spectroscopy	709:747	Fourier-transform infrared spectroscopy (FTIR)	709:754	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	4	9	theme	analytical	642:651	arg1	microscopy					832:841	scanning electron microscopy	814:841	scanning electron microscopy (SEM)	814:847	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	4	9	theme	analytical	642:651	arg1	techniques					665:674	numerous analytical and imaging techniques	633:674	numerous analytical and imaging techniques	633:674	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	4	9	theme	analytical	642:651	arg1	analysis					800:807	thermogravimetric analysis	782:807	thermogravimetric analysis	782:807	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	1	10	theme	bio-composite	176:188	arg1	development					141:151	the development	137:151	the development of chitosan (CH)-based bio-composite modified with acrylonitrile (AN) in the presence of carbon disulfide	137:257	Herein, we report the development of chitosan (CH)-based bio-composite modified with acrylonitrile (AN) in the presence of carbon disulfide.
31935863	10	11	theme	functions	1593:1601	arg1	values					1577:1582	lower values	1571:1582	lower values of error functions	1571:1601	The nonlinear regression analysis showed lower values of error functions as compared with linear regression analysis.
31935863	9	12	theme	Langmuir	1455:1462	arg1	model					1464:1468	Langmuir model	1455:1468	Langmuir model	1455:1468	Different linearization of Langmuir model showed different error functions and isothermal parameters.
31935863	10	13	theme	linear	1620:1625	arg1	analysis					1638:1645	linear regression analysis	1620:1645	linear regression analysis	1620:1645	The nonlinear regression analysis showed lower values of error functions as compared with linear regression analysis.
31935863	9	14	theme	error	1487:1491	arg1	functions					1493:1501	different error functions	1477:1501	different error functions	1477:1501	Different linearization of Langmuir model showed different error functions and isothermal parameters.
31935863	11	15	from	environment	1772:1782	arg1	decontamination					1721:1735	the decontamination	1717:1735	the decontamination of toxic elements from the aqueous environment	1717:1782	The chitosan with thiocarbamate group is an outstanding material for the decontamination of toxic elements from the aqueous environment.
31935863	11	15	from	environment	1772:1782	arg1	elements					1746:1753	toxic elements	1740:1753	toxic elements from the aqueous environment	1740:1782	The chitosan with thiocarbamate group is an outstanding material for the decontamination of toxic elements from the aqueous environment.
31935863	4	16	theme	imaging	657:663	arg1	investigation					767:779	elemental investigation	757:779	elemental investigation	757:779	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	4	16	theme	imaging	657:663	arg1	13C-NMR					687:693	13C-NMR	687:693	13C-NMR (solid-form)	687:706	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	4	16	theme	imaging	657:663	arg1	spectroscopy					736:747	Fourier-transform infrared spectroscopy	709:747	Fourier-transform infrared spectroscopy (FTIR)	709:754	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	4	16	theme	imaging	657:663	arg1	microscopy					832:841	scanning electron microscopy	814:841	scanning electron microscopy (SEM)	814:847	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	4	16	theme	imaging	657:663	arg1	techniques					665:674	numerous analytical and imaging techniques	633:674	numerous analytical and imaging techniques	633:674	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	4	16	theme	imaging	657:663	arg1	analysis					800:807	thermogravimetric analysis	782:807	thermogravimetric analysis	782:807	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	6	17	theme	sorption	970:977	arg1	ability					979:985	The sorption ability	966:985	The sorption ability of the CH-AN bio-composite	966:1012	The sorption ability of the CH-AN bio-composite was evaluated by applying it to lead and copper-containing aqueous solution.
31935863	8	18	theme	experimental	1305:1316	arg1	findings					1318:1325	The experimental findings	1301:1325	The experimental findings	1301:1325	The experimental findings fitted better to Langmuir model than Temkin and Freundlich isotherms using linear regression method.
31935863	4	19	theme	Fourier-transform	709:725	arg1	FTIR					750:753	FTIR	750:753	FTIR	750:753	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	4	19	theme	Fourier-transform	709:725	arg1	spectroscopy					736:747	Fourier-transform infrared spectroscopy	709:747	Fourier-transform infrared spectroscopy (FTIR)	709:754	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	0	20	theme	Aqueous	104:110	arg1	Media					112:116	the Aqueous Media	100:116	the Aqueous Media	100:116	Chitosan-Based Bio-Composite Modified with Thiocarbamate Moiety for Decontamination of Cations from the Aqueous Media.
31935863	5	21	theme	cations	934:940	arg1	decontamination					915:929	the decontamination	911:929	the decontamination of cations from the aqueous media	911:963	Finally, the modified bio-composite (CH-AN) was deployed for the decontamination of cations from the aqueous media.
31935863	2	22	theme	three-components	363:378	arg1	reaction					428:435	single-step three-components (chitosan, carbon disulfide, and acrylonitrile) reaction	351:435	single-step three-components (chitosan, carbon disulfide, and acrylonitrile) reaction	351:435	The current work aimed to increase the Lewis basic centers on the polymeric backbone using single-step three-components (chitosan, carbon disulfide, and acrylonitrile) reaction.
31935863	0	23	theme	Chitosan-Based	0:13	arg1	Bio-Composite					15:27	Chitosan-Based Bio-Composite	0:27	Chitosan-Based Bio-Composite	0:27	Chitosan-Based Bio-Composite Modified with Thiocarbamate Moiety for Decontamination of Cations from the Aqueous Media.
31935863	7	24	theme	sorption	1282:1289	arg1	isotherm					1291:1298	Langmuir sorption isotherm	1273:1298	Langmuir sorption isotherm	1273:1298	The chitosan-based CH-AN bio-composite exhibited greater sorption capacity for lead (2.54 mmol g-1) and copper (2.02 mmol g-1) than precursor chitosan from aqueous solution based on Langmuir sorption isotherm.
31935863	4	25	theme	infrared	727:734	arg1	FTIR					750:753	FTIR	750:753	FTIR	750:753	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	4	25	theme	infrared	727:734	arg1	spectroscopy					736:747	Fourier-transform infrared spectroscopy	709:747	Fourier-transform infrared spectroscopy (FTIR)	709:754	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	6	26	theme	bio-composite	1000:1012	arg1	ability					979:985	The sorption ability	966:985	The sorption ability of the CH-AN bio-composite	966:1012	The sorption ability of the CH-AN bio-composite was evaluated by applying it to lead and copper-containing aqueous solution.
31935863	2	27	theme	carbon	391:396	arg1	disulfide					398:406	carbon disulfide	391:406	carbon disulfide	391:406	The current work aimed to increase the Lewis basic centers on the polymeric backbone using single-step three-components (chitosan, carbon disulfide, and acrylonitrile) reaction.
31935863	0	28	theme	Thiocarbamate	43:55	arg1	Moiety					57:62	Thiocarbamate Moiety	43:62	Thiocarbamate Moiety for Decontamination of Cations from the Aqueous Media	43:116	Chitosan-Based Bio-Composite Modified with Thiocarbamate Moiety for Decontamination of Cations from the Aqueous Media.
31935863	7	29	theme	greater	1140:1146	arg1	capacity					1157:1164	greater sorption capacity	1140:1164	greater sorption capacity for lead (2.54 mmol g-1) and copper (2.02 mmol g-1)	1140:1216	The chitosan-based CH-AN bio-composite exhibited greater sorption capacity for lead (2.54 mmol g-1) and copper (2.02 mmol g-1) than precursor chitosan from aqueous solution based on Langmuir sorption isotherm.
31935863	6	30	theme	CH-AN	994:998	arg1	bio-composite					1000:1012	the CH-AN bio-composite	990:1012	the CH-AN bio-composite	990:1012	The sorption ability of the CH-AN bio-composite was evaluated by applying it to lead and copper-containing aqueous solution.
31935863	4	31	theme	elemental	757:765	arg1	investigation					767:779	elemental investigation	757:779	elemental investigation	757:779	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	2	32	theme	basic	305:309	arg1	centers					311:317	the Lewis basic centers	295:317	the Lewis basic centers on the polymeric backbone using single-step three-components (chitosan, carbon disulfide, and acrylonitrile) reaction	295:435	The current work aimed to increase the Lewis basic centers on the polymeric backbone using single-step three-components (chitosan, carbon disulfide, and acrylonitrile) reaction.
31935863	7	33	theme	precursor	1223:1231	arg1	chitosan					1233:1240	precursor chitosan	1223:1240	precursor chitosan from aqueous solution	1223:1262	The chitosan-based CH-AN bio-composite exhibited greater sorption capacity for lead (2.54 mmol g-1) and copper (2.02 mmol g-1) than precursor chitosan from aqueous solution based on Langmuir sorption isotherm.
31935863	4	34	theme	pristine	556:563	arg1	CH					565:566	the pristine CH	552:566	the pristine CH	552:566	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	11	35	theme	toxic	1740:1744	arg1	elements					1746:1753	toxic elements	1740:1753	toxic elements from the aqueous environment	1740:1782	The chitosan with thiocarbamate group is an outstanding material for the decontamination of toxic elements from the aqueous environment.
31935863	8	36	theme	regression	1409:1418	arg1	method					1420:1425	linear regression method	1402:1425	linear regression method	1402:1425	The experimental findings fitted better to Langmuir model than Temkin and Freundlich isotherms using linear regression method.
31935863	2	37	dep	three-components	363:378	arg1	chitosan					381:388	chitosan	381:388	chitosan	381:388	The current work aimed to increase the Lewis basic centers on the polymeric backbone using single-step three-components (chitosan, carbon disulfide, and acrylonitrile) reaction.
31935863	2	37	dep	three-components	363:378	arg1	disulfide					398:406	carbon disulfide	391:406	carbon disulfide	391:406	The current work aimed to increase the Lewis basic centers on the polymeric backbone using single-step three-components (chitosan, carbon disulfide, and acrylonitrile) reaction.
31935863	2	37	dep	three-components	363:378	arg1	acrylonitrile					413:425	acrylonitrile	413:425	acrylonitrile	413:425	The current work aimed to increase the Lewis basic centers on the polymeric backbone using single-step three-components (chitosan, carbon disulfide, and acrylonitrile) reaction.
31935863	6	38	theme	copper-containing	1055:1071	arg1	solution					1081:1088	copper-containing aqueous solution	1055:1088	copper-containing aqueous solution	1055:1088	The sorption ability of the CH-AN bio-composite was evaluated by applying it to lead and copper-containing aqueous solution.
31935863	10	39	theme	regression	1544:1553	arg1	analysis					1555:1562	The nonlinear regression analysis	1530:1562	The nonlinear regression analysis	1530:1562	The nonlinear regression analysis showed lower values of error functions as compared with linear regression analysis.
31935863	11	40	theme	thiocarbamate	1666:1678	arg1	group					1680:1684	thiocarbamate group	1666:1684	thiocarbamate group	1666:1684	The chitosan with thiocarbamate group is an outstanding material for the decontamination of toxic elements from the aqueous environment.
31935863	2	41	theme	polymeric	326:334	arg1	backbone					336:343	the polymeric backbone	322:343	the polymeric backbone using single-step three-components (chitosan, carbon disulfide, and acrylonitrile) reaction	322:435	The current work aimed to increase the Lewis basic centers on the polymeric backbone using single-step three-components (chitosan, carbon disulfide, and acrylonitrile) reaction.
31935863	11	42	with	chitosan	1652:1659	arg1	group					1680:1684	thiocarbamate group	1666:1684	thiocarbamate group	1666:1684	The chitosan with thiocarbamate group is an outstanding material for the decontamination of toxic elements from the aqueous environment.
31935863	7	43	theme	mmol	1208:1211	arg1	g-1					1213:1215	2.02 mmol g-1	1203:1215	2.02 mmol g-1	1203:1215	The chitosan-based CH-AN bio-composite exhibited greater sorption capacity for lead (2.54 mmol g-1) and copper (2.02 mmol g-1) than precursor chitosan from aqueous solution based on Langmuir sorption isotherm.
31935863	7	43	theme	mmol	1208:1211	arg1	copper					1195:1200	copper	1195:1200	copper (2.02 mmol g-1)	1195:1216	The chitosan-based CH-AN bio-composite exhibited greater sorption capacity for lead (2.54 mmol g-1) and copper (2.02 mmol g-1) than precursor chitosan from aqueous solution based on Langmuir sorption isotherm.
31935863	10	44	theme	error	1587:1591	arg1	functions					1593:1601	error functions	1587:1601	error functions	1587:1601	The nonlinear regression analysis showed lower values of error functions as compared with linear regression analysis.
31935863	3	45	theme	functional	511:520	arg1	NH2					529:531	NH2	529:531	NH2	529:531	For a said purpose, the thiocarbamate moiety was attached to the pendant functional amine (NH2) of chitosan.
31935863	3	45	theme	functional	511:520	arg1	amine					522:526	the pendant functional amine	499:526	the pendant functional amine (NH2) of chitosan	499:544	For a said purpose, the thiocarbamate moiety was attached to the pendant functional amine (NH2) of chitosan.
31935863	1	46	theme	carbon	242:247	arg1	disulfide					249:257	carbon disulfide	242:257	carbon disulfide	242:257	Herein, we report the development of chitosan (CH)-based bio-composite modified with acrylonitrile (AN) in the presence of carbon disulfide.
31935863	3	47	attach	attached	487:494	arg1	NH2					529:531	NH2	529:531	NH2	529:531	For a said purpose, the thiocarbamate moiety was attached to the pendant functional amine (NH2) of chitosan.
31935863	3	47	attach	attached	487:494	arg1	amine					522:526	the pendant functional amine	499:526	the pendant functional amine (NH2) of chitosan	499:544	For a said purpose, the thiocarbamate moiety was attached to the pendant functional amine (NH2) of chitosan.
31935863	3	47	attach	attached	487:494	arg2	moiety					476:481	the thiocarbamate moiety	458:481	the thiocarbamate moiety	458:481	For a said purpose, the thiocarbamate moiety was attached to the pendant functional amine (NH2) of chitosan.
31935863	8	48	dep	Temkin	1364:1369	arg1	isotherms					1386:1394	isotherms	1386:1394	isotherms	1386:1394	The experimental findings fitted better to Langmuir model than Temkin and Freundlich isotherms using linear regression method.
31935863	11	49	theme	outstanding	1692:1702	arg1	material					1704:1711	an outstanding material	1689:1711	an outstanding material for the decontamination of toxic elements from the aqueous environment	1689:1782	The chitosan with thiocarbamate group is an outstanding material for the decontamination of toxic elements from the aqueous environment.
31935863	11	49	theme	outstanding	1692:1702	arg1	chitosan					1652:1659	The chitosan	1648:1659	The chitosan with thiocarbamate group	1648:1684	The chitosan with thiocarbamate group is an outstanding material for the decontamination of toxic elements from the aqueous environment.
31935863	9	50	theme	Different	1428:1436	arg1	linearization					1438:1450	Different linearization	1428:1450	Different linearization of Langmuir model	1428:1468	Different linearization of Langmuir model showed different error functions and isothermal parameters.
31935863	8	51	dep	model	1353:1357	arg1	to					1341:1342	to	1341:1342	to	1341:1342	The experimental findings fitted better to Langmuir model than Temkin and Freundlich isotherms using linear regression method.
31935863	4	52	theme	scanning	814:821	arg1	microscopy					832:841	scanning electron microscopy	814:841	scanning electron microscopy (SEM)	814:847	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	4	52	theme	scanning	814:821	arg1	SEM					844:846	SEM	844:846	SEM	844:846	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	5	53	from	media	959:963	arg1	decontamination					915:929	the decontamination	911:929	the decontamination of cations from the aqueous media	911:963	Finally, the modified bio-composite (CH-AN) was deployed for the decontamination of cations from the aqueous media.
31935863	1	54	theme	-based	169:174	arg1	bio-composite					176:188	chitosan (CH)-based bio-composite	156:188	chitosan (CH)-based bio-composite modified with acrylonitrile (AN) in the presence of carbon disulfide	156:257	Herein, we report the development of chitosan (CH)-based bio-composite modified with acrylonitrile (AN) in the presence of carbon disulfide.
31935863	4	55	theme	numerous	633:640	arg1	investigation					767:779	elemental investigation	757:779	elemental investigation	757:779	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	4	55	theme	numerous	633:640	arg1	13C-NMR					687:693	13C-NMR	687:693	13C-NMR (solid-form)	687:706	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	4	55	theme	numerous	633:640	arg1	spectroscopy					736:747	Fourier-transform infrared spectroscopy	709:747	Fourier-transform infrared spectroscopy (FTIR)	709:754	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	4	55	theme	numerous	633:640	arg1	microscopy					832:841	scanning electron microscopy	814:841	scanning electron microscopy (SEM)	814:847	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	4	55	theme	numerous	633:640	arg1	techniques					665:674	numerous analytical and imaging techniques	633:674	numerous analytical and imaging techniques	633:674	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	4	55	theme	numerous	633:640	arg1	analysis					800:807	thermogravimetric analysis	782:807	thermogravimetric analysis	782:807	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	4	56	theme	CH-AN	581:585	arg1	bio-composites					587:600	Both the pristine CH and modified CH-AN bio-composites	547:600	Both the pristine CH and modified CH-AN bio-composites	547:600	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	9	57	theme	model	1464:1468	arg1	linearization					1438:1450	Different linearization	1428:1450	Different linearization of Langmuir model	1428:1468	Different linearization of Langmuir model showed different error functions and isothermal parameters.
31935863	10	58	theme	regression	1627:1636	arg1	analysis					1638:1645	linear regression analysis	1620:1645	linear regression analysis	1620:1645	The nonlinear regression analysis showed lower values of error functions as compared with linear regression analysis.
31935863	3	59	theme	chitosan	537:544	arg1	NH2					529:531	NH2	529:531	NH2	529:531	For a said purpose, the thiocarbamate moiety was attached to the pendant functional amine (NH2) of chitosan.
31935863	3	59	theme	chitosan	537:544	arg1	amine					522:526	the pendant functional amine	499:526	the pendant functional amine (NH2) of chitosan	499:544	For a said purpose, the thiocarbamate moiety was attached to the pendant functional amine (NH2) of chitosan.
31935863	9	60	theme	different	1477:1485	arg1	functions					1493:1501	different error functions	1477:1501	different error functions	1477:1501	Different linearization of Langmuir model showed different error functions and isothermal parameters.
31935863	1	61	dep	acrylonitrile	204:216	arg1	AN					219:220	AN	219:220	AN	219:220	Herein, we report the development of chitosan (CH)-based bio-composite modified with acrylonitrile (AN) in the presence of carbon disulfide.
31935863	0	62	from	Media	112:116	arg1	Decontamination					68:82	Decontamination	68:82	Decontamination of Cations from the Aqueous Media	68:116	Chitosan-Based Bio-Composite Modified with Thiocarbamate Moiety for Decontamination of Cations from the Aqueous Media.
31935863	0	63	theme	Cations	87:93	arg1	Decontamination					68:82	Decontamination	68:82	Decontamination of Cations from the Aqueous Media	68:116	Chitosan-Based Bio-Composite Modified with Thiocarbamate Moiety for Decontamination of Cations from the Aqueous Media.
31935863	9	64	theme	isothermal	1507:1516	arg1	parameters					1518:1527	isothermal parameters	1507:1527	isothermal parameters	1507:1527	Different linearization of Langmuir model showed different error functions and isothermal parameters.
31935863	11	65	theme	aqueous	1764:1770	arg1	environment					1772:1782	the aqueous environment	1760:1782	the aqueous environment	1760:1782	The chitosan with thiocarbamate group is an outstanding material for the decontamination of toxic elements from the aqueous environment.
31935863	5	66	theme	modified	863:870	arg1	CH-AN					887:891	CH-AN	887:891	CH-AN	887:891	Finally, the modified bio-composite (CH-AN) was deployed for the decontamination of cations from the aqueous media.
31935863	5	66	theme	modified	863:870	arg1	bio-composite					872:884	the modified bio-composite	859:884	the modified bio-composite (CH-AN)	859:892	Finally, the modified bio-composite (CH-AN) was deployed for the decontamination of cations from the aqueous media.
31935863	7	67	theme	mmol	1181:1184	arg1	g-1					1186:1188	2.54 mmol g-1	1176:1188	2.54 mmol g-1	1176:1188	The chitosan-based CH-AN bio-composite exhibited greater sorption capacity for lead (2.54 mmol g-1) and copper (2.02 mmol g-1) than precursor chitosan from aqueous solution based on Langmuir sorption isotherm.
31935863	7	67	theme	mmol	1181:1184	arg1	lead					1170:1173	lead	1170:1173	lead (2.54 mmol g-1)	1170:1189	The chitosan-based CH-AN bio-composite exhibited greater sorption capacity for lead (2.54 mmol g-1) and copper (2.02 mmol g-1) than precursor chitosan from aqueous solution based on Langmuir sorption isotherm.
31935863	11	68	from	decontamination	1721:1735	arg1	environment					1772:1782	the aqueous environment	1760:1782	the aqueous environment	1760:1782	The chitosan with thiocarbamate group is an outstanding material for the decontamination of toxic elements from the aqueous environment.
31935863	7	69	theme	Langmuir	1273:1280	arg1	isotherm					1291:1298	Langmuir sorption isotherm	1273:1298	Langmuir sorption isotherm	1273:1298	The chitosan-based CH-AN bio-composite exhibited greater sorption capacity for lead (2.54 mmol g-1) and copper (2.02 mmol g-1) than precursor chitosan from aqueous solution based on Langmuir sorption isotherm.
31935863	8	70	theme	better	1334:1339	arg1	model					1353:1357	better to Langmuir model	1334:1357	better to Langmuir model than Temkin and Freundlich isotherms	1334:1394	The experimental findings fitted better to Langmuir model than Temkin and Freundlich isotherms using linear regression method.
31935863	2	71	theme	single-step	351:361	arg1	three-components					363:378	single-step three-components	351:378	single-step three-components (chitosan, carbon disulfide, and acrylonitrile) reaction	351:435	The current work aimed to increase the Lewis basic centers on the polymeric backbone using single-step three-components (chitosan, carbon disulfide, and acrylonitrile) reaction.
31935863	7	72	theme	sorption	1148:1155	arg1	capacity					1157:1164	greater sorption capacity	1140:1164	greater sorption capacity for lead (2.54 mmol g-1) and copper (2.02 mmol g-1)	1140:1216	The chitosan-based CH-AN bio-composite exhibited greater sorption capacity for lead (2.54 mmol g-1) and copper (2.02 mmol g-1) than precursor chitosan from aqueous solution based on Langmuir sorption isotherm.
31935863	8	73	theme	Langmuir	1344:1351	arg1	model					1353:1357	better to Langmuir model	1334:1357	better to Langmuir model than Temkin and Freundlich isotherms	1334:1394	The experimental findings fitted better to Langmuir model than Temkin and Freundlich isotherms using linear regression method.
31935863	3	74	theme	said	444:447	arg1	purpose					449:455	a said purpose	442:455	a said purpose	442:455	For a said purpose, the thiocarbamate moiety was attached to the pendant functional amine (NH2) of chitosan.
31935863	7	75	theme	aqueous	1247:1253	arg1	solution					1255:1262	aqueous solution	1247:1262	aqueous solution	1247:1262	The chitosan-based CH-AN bio-composite exhibited greater sorption capacity for lead (2.54 mmol g-1) and copper (2.02 mmol g-1) than precursor chitosan from aqueous solution based on Langmuir sorption isotherm.
31935863	4	76	theme	thermogravimetric	782:798	arg1	analysis					800:807	thermogravimetric analysis	782:807	thermogravimetric analysis	782:807	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	7	77	theme	CH-AN	1110:1114	arg1	bio-composite					1116:1128	The chitosan-based CH-AN bio-composite	1091:1128	The chitosan-based CH-AN bio-composite	1091:1128	The chitosan-based CH-AN bio-composite exhibited greater sorption capacity for lead (2.54 mmol g-1) and copper (2.02 mmol g-1) than precursor chitosan from aqueous solution based on Langmuir sorption isotherm.
31935863	7	78	from	solution	1255:1262	arg1	chitosan					1233:1240	precursor chitosan	1223:1240	precursor chitosan from aqueous solution	1223:1262	The chitosan-based CH-AN bio-composite exhibited greater sorption capacity for lead (2.54 mmol g-1) and copper (2.02 mmol g-1) than precursor chitosan from aqueous solution based on Langmuir sorption isotherm.
31935863	11	79	theme	elements	1746:1753	arg1	decontamination					1721:1735	the decontamination	1717:1735	the decontamination of toxic elements from the aqueous environment	1717:1782	The chitosan with thiocarbamate group is an outstanding material for the decontamination of toxic elements from the aqueous environment.
31935863	3	80	theme	thiocarbamate	462:474	arg1	moiety					476:481	the thiocarbamate moiety	458:481	the thiocarbamate moiety	458:481	For a said purpose, the thiocarbamate moiety was attached to the pendant functional amine (NH2) of chitosan.
31935863	5	81	theme	aqueous	951:957	arg1	media					959:963	the aqueous media	947:963	the aqueous media	947:963	Finally, the modified bio-composite (CH-AN) was deployed for the decontamination of cations from the aqueous media.
31935863	4	82	theme	CH	565:566	arg1	bio-composites					587:600	Both the pristine CH and modified CH-AN bio-composites	547:600	Both the pristine CH and modified CH-AN bio-composites	547:600	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	2	83	theme	Lewis	299:303	arg1	centers					311:317	the Lewis basic centers	295:317	the Lewis basic centers on the polymeric backbone using single-step three-components (chitosan, carbon disulfide, and acrylonitrile) reaction	295:435	The current work aimed to increase the Lewis basic centers on the polymeric backbone using single-step three-components (chitosan, carbon disulfide, and acrylonitrile) reaction.
31935863	6	84	theme	aqueous	1073:1079	arg1	solution					1081:1088	copper-containing aqueous solution	1055:1088	copper-containing aqueous solution	1055:1088	The sorption ability of the CH-AN bio-composite was evaluated by applying it to lead and copper-containing aqueous solution.
31935863	4	85	theme	modified	572:579	arg1	CH-AN					581:585	modified CH-AN	572:585	modified CH-AN	572:585	Both the pristine CH and modified CH-AN bio-composites were first characterized using numerous analytical and imaging techniques, including 13C-NMR (solid-form), Fourier-transform infrared spectroscopy (FTIR), elemental investigation, thermogravimetric analysis, and scanning electron microscopy (SEM).
31935863	8	86	theme	linear	1402:1407	arg1	method					1420:1425	linear regression method	1402:1425	linear regression method	1402:1425	The experimental findings fitted better to Langmuir model than Temkin and Freundlich isotherms using linear regression method.
34071909	9	0	theme	HS	1203:1204	arg1	composition					1206:1216	HS composition	1203:1216	HS composition	1203:1216	No significant changes in HS composition were noted after AB.
34071909	0	1	from	Deposition	21:30	arg1	Mice					76:79	Mice	76:79	Mice	76:79	Extracellular Matrix Deposition and Remodeling after Corneal Alkali Burn in Mice.
34071909	7	2	theme	decorin	990:996	arg1	expression					998:1007	decorin expression	990:1007	decorin expression	990:1007	Expression of the CSPGs biglycan and versican was increased after AB, while decorin expression was decreased.
34071909	6	3	theme	overall	793:799	arg1	increase					801:808	a significant overall increase	779:808	a significant overall increase in CS	779:814	We found a significant overall increase in CS after AB, with an increase in sulfated forms of CS and a decrease in lesser sulfated forms of CS.
34071909	1	4	theme	collagen	156:163	arg1	fibrils					165:171	collagen fibrils	156:171	collagen fibrils	156:171	Corneal transparency relies on the precise arrangement and orientation of collagen fibrils, made of mostly Type I and V collagen fibrils and proteoglycans (PGs).
34071909	6	5	theme	lesser	885:890	arg1	forms					901:905	lesser sulfated forms	885:905	lesser sulfated forms of CS	885:911	We found a significant overall increase in CS after AB, with an increase in sulfated forms of CS and a decrease in lesser sulfated forms of CS.
34071909	4	6	theme	sulfate	492:498	arg1	composition					465:475	The chemical composition	452:475	The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS)	452:550	The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas.
34071909	8	7	from	decrease	1143:1150	arg1	expression					1165:1174	keratocan expression	1155:1174	keratocan expression	1155:1174	We also found an increase in KS expression 14 days after AB, with an increase in lumican and mimecan expression, and a decrease in keratocan expression.
34071909	8	7	from	decrease	1143:1150	arg1	lumican					1105:1111	lumican	1105:1111	lumican	1105:1111	We also found an increase in KS expression 14 days after AB, with an increase in lumican and mimecan expression, and a decrease in keratocan expression.
34071909	8	7	from	decrease	1143:1150	arg1	expression					1125:1134	mimecan expression	1117:1134	mimecan expression	1117:1134	We also found an increase in KS expression 14 days after AB, with an increase in lumican and mimecan expression, and a decrease in keratocan expression.
34071909	1	8	theme	fibrils	165:171	arg1	arrangement					125:135	precise arrangement	117:135	precise arrangement	117:135	Corneal transparency relies on the precise arrangement and orientation of collagen fibrils, made of mostly Type I and V collagen fibrils and proteoglycans (PGs).
34071909	1	8	theme	fibrils	165:171	arg1	orientation					141:151	orientation	141:151	orientation	141:151	Corneal transparency relies on the precise arrangement and orientation of collagen fibrils, made of mostly Type I and V collagen fibrils and proteoglycans (PGs).
34071909	10	9	theme	matrix	1333:1338	arg1	composition					1300:1310	the composition	1296:1310	the composition of the extracellular matrix	1296:1338	Taken together, our study reveals significant changes in the composition of the extracellular matrix following a corneal chemical injury.
34071909	6	10	from	increase	801:808	arg1	CS					813:814	CS	813:814	CS	813:814	We found a significant overall increase in CS after AB, with an increase in sulfated forms of CS and a decrease in lesser sulfated forms of CS.
34071909	10	11	theme	significant	1273:1283	arg1	changes					1285:1291	significant changes	1273:1291	significant changes in the composition of the extracellular matrix	1273:1338	Taken together, our study reveals significant changes in the composition of the extracellular matrix following a corneal chemical injury.
34071909	1	12	dep	arrangement	125:135	arg1	the					113:115	the	113:115	the	113:115	Corneal transparency relies on the precise arrangement and orientation of collagen fibrils, made of mostly Type I and V collagen fibrils and proteoglycans (PGs).
34071909	3	13	theme	spatial	356:362	arg1	distribution					377:388	the spatial and temporal distribution	352:388	the spatial and temporal distribution of glycosaminoglycans (GAGs) and PGs	352:425	We investigated the spatial and temporal distribution of glycosaminoglycans (GAGs) and PGs after a chemical injury.
34071909	6	14	theme	sulfated	892:899	arg1	forms					901:905	lesser sulfated forms	885:905	lesser sulfated forms of CS	885:911	We found a significant overall increase in CS after AB, with an increase in sulfated forms of CS and a decrease in lesser sulfated forms of CS.
34071909	5	15	theme	expression	665:674	arg1	profile					676:682	expression profile	665:682	expression profile	665:682	The expression profile and corneal distribution of CS/DSPGs and keratan sulfate (KS) PGs were also analyzed.
34071909	4	16	theme	chemical	456:463	arg1	composition					465:475	The chemical composition	452:475	The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS)	452:550	The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas.
34071909	6	17	from	decrease	873:880	arg1	forms					901:905	lesser sulfated forms	885:905	lesser sulfated forms of CS	885:911	We found a significant overall increase in CS after AB, with an increase in sulfated forms of CS and a decrease in lesser sulfated forms of CS.
34071909	6	17	from	decrease	873:880	arg1	forms					855:859	sulfated forms	846:859	sulfated forms of CS	846:865	We found a significant overall increase in CS after AB, with an increase in sulfated forms of CS and a decrease in lesser sulfated forms of CS.
34071909	4	18	theme	alkali	608:613	arg1	AB					621:622	AB	621:622	AB	621:622	The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas.
34071909	4	18	theme	alkali	608:613	arg1	burn					615:618	alkali burn	608:618	alkali burn (AB)	608:623	The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas.
34071909	5	19	theme	sulfate	733:739	arg1	PGs					746:748	keratan sulfate (KS) PGs	725:748	keratan sulfate (KS) PGs	725:748	The expression profile and corneal distribution of CS/DSPGs and keratan sulfate (KS) PGs were also analyzed.
34071909	4	20	theme	chondroitin	480:490	arg1	CS					501:502	CS	501:502	CS	501:502	The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas.
34071909	4	20	theme	chondroitin	480:490	arg1	sulfate					492:498	chondroitin sulfate	480:498	chondroitin sulfate (CS)	480:503	The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas.
34071909	10	21	theme	extracellular	1319:1331	arg1	matrix					1333:1338	the extracellular matrix	1315:1338	the extracellular matrix	1315:1338	Taken together, our study reveals significant changes in the composition of the extracellular matrix following a corneal chemical injury.
34071909	0	22	theme	Matrix	14:19	arg1	Deposition					21:30	Extracellular Matrix Deposition	0:30	Extracellular Matrix Deposition	0:30	Extracellular Matrix Deposition and Remodeling after Corneal Alkali Burn in Mice.
34071909	0	23	from	Remodeling	36:45	arg1	Mice					76:79	Mice	76:79	Mice	76:79	Extracellular Matrix Deposition and Remodeling after Corneal Alkali Burn in Mice.
34071909	1	24	theme	Corneal	82:88	arg1	transparency					90:101	Corneal transparency	82:101	Corneal transparency	82:101	Corneal transparency relies on the precise arrangement and orientation of collagen fibrils, made of mostly Type I and V collagen fibrils and proteoglycans (PGs).
34071909	0	25	theme	Extracellular	0:12	arg1	Deposition					21:30	Extracellular Matrix Deposition	0:30	Extracellular Matrix Deposition	0:30	Extracellular Matrix Deposition and Remodeling after Corneal Alkali Burn in Mice.
34071909	9	26	from	changes	1192:1198	arg1	composition					1206:1216	HS composition	1203:1216	HS composition	1203:1216	No significant changes in HS composition were noted after AB.
34071909	3	27	theme	PGs	423:425	arg1	distribution					377:388	the spatial and temporal distribution	352:388	the spatial and temporal distribution of glycosaminoglycans (GAGs) and PGs	352:425	We investigated the spatial and temporal distribution of glycosaminoglycans (GAGs) and PGs after a chemical injury.
34071909	6	28	theme	CS	864:865	arg1	forms					855:859	sulfated forms	846:859	sulfated forms of CS	846:865	We found a significant overall increase in CS after AB, with an increase in sulfated forms of CS and a decrease in lesser sulfated forms of CS.
34071909	2	29	theme	collagen	274:281	arg1	fibrillogenesis					283:297	correct collagen fibrillogenesis	266:297	correct collagen fibrillogenesis	266:297	PGs are essential for correct collagen fibrillogenesis and maintaining corneal homeostasis.
34071909	1	30	theme	Type	189:192	arg1	fibrils					211:217	mostly Type I and V collagen fibrils	182:217	mostly Type I and V collagen fibrils	182:217	Corneal transparency relies on the precise arrangement and orientation of collagen fibrils, made of mostly Type I and V collagen fibrils and proteoglycans (PGs).
34071909	3	31	theme	temporal	368:375	arg1	distribution					377:388	the spatial and temporal distribution	352:388	the spatial and temporal distribution of glycosaminoglycans (GAGs) and PGs	352:425	We investigated the spatial and temporal distribution of glycosaminoglycans (GAGs) and PGs after a chemical injury.
34071909	4	32	theme	sulfate	539:545	arg1	composition					465:475	The chemical composition	452:475	The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS)	452:550	The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas.
34071909	6	33	theme	significant	781:791	arg1	increase					801:808	a significant overall increase	779:808	a significant overall increase in CS	779:814	We found a significant overall increase in CS after AB, with an increase in sulfated forms of CS and a decrease in lesser sulfated forms of CS.
34071909	2	34	theme	correct	266:272	arg1	fibrillogenesis					283:297	correct collagen fibrillogenesis	266:297	correct collagen fibrillogenesis	266:297	PGs are essential for correct collagen fibrillogenesis and maintaining corneal homeostasis.
34071909	5	35	theme	CS/DSPGs	712:719	arg1	profile					676:682	expression profile	665:682	expression profile	665:682	The expression profile and corneal distribution of CS/DSPGs and keratan sulfate (KS) PGs were also analyzed.
34071909	5	35	theme	CS/DSPGs	712:719	arg1	distribution					696:707	corneal distribution	688:707	corneal distribution	688:707	The expression profile and corneal distribution of CS/DSPGs and keratan sulfate (KS) PGs were also analyzed.
34071909	4	36	theme	heparan	531:537	arg1	HS					548:549	HS	548:549	HS	548:549	The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas.
34071909	4	36	theme	heparan	531:537	arg1	sulfate					539:545	heparan sulfate	531:545	heparan sulfate (HS)	531:550	The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas.
34071909	7	37	theme	CSPGs	932:936	arg1	Expression					914:923	Expression	914:923	Expression of the CSPGs biglycan and versican	914:958	Expression of the CSPGs biglycan and versican was increased after AB, while decorin expression was decreased.
34071909	4	38	dep	days	597:600	arg1	AB					621:622	AB	621:622	AB	621:622	The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas.
34071909	4	38	dep	days	597:600	arg1	burn					615:618	alkali burn	608:618	alkali burn (AB)	608:623	The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas.
34071909	3	39	theme	chemical	435:442	arg1	injury					444:449	a chemical injury	433:449	a chemical injury	433:449	We investigated the spatial and temporal distribution of glycosaminoglycans (GAGs) and PGs after a chemical injury.
34071909	6	40	theme	sulfated	846:853	arg1	forms					855:859	sulfated forms	846:859	sulfated forms of CS	846:865	We found a significant overall increase in CS after AB, with an increase in sulfated forms of CS and a decrease in lesser sulfated forms of CS.
34071909	8	41	dep	days	1070:1073	arg1	AB					1081:1082	AB	1081:1082	AB	1081:1082	We also found an increase in KS expression 14 days after AB, with an increase in lumican and mimecan expression, and a decrease in keratocan expression.
34071909	9	42	theme	significant	1180:1190	arg1	changes					1192:1198	No significant changes	1177:1198	No significant changes in HS composition	1177:1216	No significant changes in HS composition were noted after AB.
34071909	1	43	theme	V	200:200	arg1	collagen					202:209	V collagen	200:209	V collagen	200:209	Corneal transparency relies on the precise arrangement and orientation of collagen fibrils, made of mostly Type I and V collagen fibrils and proteoglycans (PGs).
34071909	0	44	theme	Alkali	61:66	arg1	Burn					68:71	Corneal Alkali Burn	53:71	Corneal Alkali Burn	53:71	Extracellular Matrix Deposition and Remodeling after Corneal Alkali Burn in Mice.
34071909	4	45	theme	/dermatan	504:512	arg1	DS					523:524	DS	523:524	DS	523:524	The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas.
34071909	4	45	theme	/dermatan	504:512	arg1	sulfate					514:520	/dermatan sulfate	504:520	/dermatan sulfate (DS)	504:525	The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas.
34071909	6	46	from	increase	834:841	arg1	forms					901:905	lesser sulfated forms	885:905	lesser sulfated forms of CS	885:911	We found a significant overall increase in CS after AB, with an increase in sulfated forms of CS and a decrease in lesser sulfated forms of CS.
34071909	6	46	from	increase	834:841	arg1	forms					855:859	sulfated forms	846:859	sulfated forms of CS	846:865	We found a significant overall increase in CS after AB, with an increase in sulfated forms of CS and a decrease in lesser sulfated forms of CS.
34071909	5	47	theme	corneal	688:694	arg1	distribution					696:707	corneal distribution	688:707	corneal distribution	688:707	The expression profile and corneal distribution of CS/DSPGs and keratan sulfate (KS) PGs were also analyzed.
34071909	8	48	from	increase	1093:1100	arg1	expression					1165:1174	keratocan expression	1155:1174	keratocan expression	1155:1174	We also found an increase in KS expression 14 days after AB, with an increase in lumican and mimecan expression, and a decrease in keratocan expression.
34071909	8	48	from	increase	1093:1100	arg1	lumican					1105:1111	lumican	1105:1111	lumican	1105:1111	We also found an increase in KS expression 14 days after AB, with an increase in lumican and mimecan expression, and a decrease in keratocan expression.
34071909	8	48	from	increase	1093:1100	arg1	expression					1125:1134	mimecan expression	1117:1134	mimecan expression	1117:1134	We also found an increase in KS expression 14 days after AB, with an increase in lumican and mimecan expression, and a decrease in keratocan expression.
34071909	10	49	from	changes	1285:1291	arg1	composition					1300:1310	the composition	1296:1310	the composition of the extracellular matrix	1296:1338	Taken together, our study reveals significant changes in the composition of the extracellular matrix following a corneal chemical injury.
34071909	0	50	theme	Corneal	53:59	arg1	Burn					68:71	Corneal Alkali Burn	53:71	Corneal Alkali Burn	53:71	Extracellular Matrix Deposition and Remodeling after Corneal Alkali Burn in Mice.
34071909	5	51	dep	profile	676:682	arg1	The					661:663	The	661:663	The	661:663	The expression profile and corneal distribution of CS/DSPGs and keratan sulfate (KS) PGs were also analyzed.
34071909	8	52	from	increase	1041:1048	arg1	expression					1056:1065	KS expression	1053:1065	KS expression	1053:1065	We also found an increase in KS expression 14 days after AB, with an increase in lumican and mimecan expression, and a decrease in keratocan expression.
34071909	1	53	theme	precise	117:123	arg1	arrangement					125:135	precise arrangement	117:135	precise arrangement	117:135	Corneal transparency relies on the precise arrangement and orientation of collagen fibrils, made of mostly Type I and V collagen fibrils and proteoglycans (PGs).
34071909	4	54	dep	sulfate	492:498	arg1	DS					523:524	DS	523:524	DS	523:524	The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas.
34071909	4	54	dep	sulfate	492:498	arg1	sulfate					514:520	/dermatan sulfate	504:520	/dermatan sulfate (DS)	504:525	The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas.
34071909	10	55	theme	chemical	1360:1367	arg1	injury					1369:1374	a corneal chemical injury	1350:1374	a corneal chemical injury	1350:1374	Taken together, our study reveals significant changes in the composition of the extracellular matrix following a corneal chemical injury.
34071909	5	56	theme	keratan	725:731	arg1	KS					742:743	KS	742:743	KS	742:743	The expression profile and corneal distribution of CS/DSPGs and keratan sulfate (KS) PGs were also analyzed.
34071909	5	56	theme	keratan	725:731	arg1	sulfate					733:739	keratan sulfate	725:739	keratan sulfate (KS) PGs	725:748	The expression profile and corneal distribution of CS/DSPGs and keratan sulfate (KS) PGs were also analyzed.
34071909	2	57	theme	corneal	315:321	arg1	homeostasis					323:333	corneal homeostasis	315:333	corneal homeostasis	315:333	PGs are essential for correct collagen fibrillogenesis and maintaining corneal homeostasis.
34071909	1	58	theme	collagen	202:209	arg1	fibrils					211:217	mostly Type I and V collagen fibrils	182:217	mostly Type I and V collagen fibrils	182:217	Corneal transparency relies on the precise arrangement and orientation of collagen fibrils, made of mostly Type I and V collagen fibrils and proteoglycans (PGs).
34071909	10	59	theme	corneal	1352:1358	arg1	injury					1369:1374	a corneal chemical injury	1350:1374	a corneal chemical injury	1350:1374	Taken together, our study reveals significant changes in the composition of the extracellular matrix following a corneal chemical injury.
34071909	4	60	theme	uninjured	642:650	arg1	corneas					652:658	uninjured corneas	642:658	uninjured corneas	642:658	The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas.
34071909	8	61	theme	KS	1053:1054	arg1	expression					1056:1065	KS expression	1053:1065	KS expression	1053:1065	We also found an increase in KS expression 14 days after AB, with an increase in lumican and mimecan expression, and a decrease in keratocan expression.
34071909	7	62	dep	CSPGs	932:936	arg1	biglycan					938:945	biglycan	938:945	biglycan	938:945	Expression of the CSPGs biglycan and versican was increased after AB, while decorin expression was decreased.
34071909	7	62	dep	CSPGs	932:936	arg1	CSPGs					932:936	the CSPGs biglycan and versican	928:958	the CSPGs biglycan and versican	928:958	Expression of the CSPGs biglycan and versican was increased after AB, while decorin expression was decreased.
34071909	7	62	dep	CSPGs	932:936	arg1	versican					951:958	versican	951:958	versican	951:958	Expression of the CSPGs biglycan and versican was increased after AB, while decorin expression was decreased.
34071909	8	63	theme	mimecan	1117:1123	arg1	expression					1125:1134	mimecan expression	1117:1134	mimecan expression	1117:1134	We also found an increase in KS expression 14 days after AB, with an increase in lumican and mimecan expression, and a decrease in keratocan expression.
34071909	8	64	theme	keratocan	1155:1163	arg1	expression					1165:1174	keratocan expression	1155:1174	keratocan expression	1155:1174	We also found an increase in KS expression 14 days after AB, with an increase in lumican and mimecan expression, and a decrease in keratocan expression.
34071909	3	65	theme	glycosaminoglycans	393:410	arg1	distribution					377:388	the spatial and temporal distribution	352:388	the spatial and temporal distribution of glycosaminoglycans (GAGs) and PGs	352:425	We investigated the spatial and temporal distribution of glycosaminoglycans (GAGs) and PGs after a chemical injury.
34071909	5	66	theme	PGs	746:748	arg1	profile					676:682	expression profile	665:682	expression profile	665:682	The expression profile and corneal distribution of CS/DSPGs and keratan sulfate (KS) PGs were also analyzed.
34071909	5	66	theme	PGs	746:748	arg1	distribution					696:707	corneal distribution	688:707	corneal distribution	688:707	The expression profile and corneal distribution of CS/DSPGs and keratan sulfate (KS) PGs were also analyzed.
34071909	6	67	theme	CS	910:911	arg1	forms					901:905	lesser sulfated forms	885:905	lesser sulfated forms of CS	885:911	We found a significant overall increase in CS after AB, with an increase in sulfated forms of CS and a decrease in lesser sulfated forms of CS.
34071909	4	68	theme	mouse	574:578	arg1	corneas					580:586	mouse corneas	574:586	mouse corneas	574:586	The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas.
32156543	0	0	theme	cells	96:100	arg1	viability					62:70	cell viability	57:70	cell viability of cryopreserved SaOS-2 cells	57:100	Gelatin carrageenan sericin hydrogel composites improves cell viability of cryopreserved SaOS-2 cells.
32156543	5	1	theme	drug	816:819	arg1	profile					829:835	sustained drug release profile	806:835	sustained drug release profile	806:835	Further, silver nanoparticles, alendronate and insulin like growth factor (IGF-1) were incorporated into the hybrid hydrogels individually and checked for sustained drug release profile.
32156543	1	2	from	challenge	184:192	arg1	therapies					226:234	cell based bone regeneration therapies	197:234	cell based bone regeneration therapies	197:234	Cryopreservation and the low revival rate of cryopreserved cells remains a major challenge in cell based bone regeneration therapies.
32156543	0	3	theme	SaOS-2	89:94	arg1	cells					96:100	cryopreserved SaOS-2 cells	75:100	cryopreserved SaOS-2 cells	75:100	Gelatin carrageenan sericin hydrogel composites improves cell viability of cryopreserved SaOS-2 cells.
32156543	8	4	dep	%	1234:1234	arg1	80					1232:1233	80	1232:1233	80	1232:1233	A flow cytometry based staining protocol using Annexin V was developed which showed a live cell population up to 80% even after 30 days of crypreservation.
32156543	6	5	theme	better	885:890	arg1	attachment					908:917	better osteogenic cell attachment	885:917	better osteogenic cell attachment	885:917	IGF-1 incorporated hydrogels composites showed better osteogenic cell attachment, proliferation and cell revival upon cryopreservation.
32156543	5	6	theme	growth	711:716	arg1	IGF-1					726:730	IGF-1	726:730	IGF-1	726:730	Further, silver nanoparticles, alendronate and insulin like growth factor (IGF-1) were incorporated into the hybrid hydrogels individually and checked for sustained drug release profile.
32156543	5	6	theme	growth	711:716	arg1	factor					718:723	growth factor	711:723	growth factor (IGF-1)	711:731	Further, silver nanoparticles, alendronate and insulin like growth factor (IGF-1) were incorporated into the hybrid hydrogels individually and checked for sustained drug release profile.
32156543	6	7	theme	cell	938:941	arg1	revival					943:949	cell revival	938:949	cell revival	938:949	IGF-1 incorporated hydrogels composites showed better osteogenic cell attachment, proliferation and cell revival upon cryopreservation.
32156543	2	8	theme	based	288:292	arg1	composite					303:311	a sericin based hydrogel composite	278:311	a sericin based hydrogel composite incorporating various drugs and growth factors to enhance cell attachment	278:385	In our current study we aimed to develop a sericin based hydrogel composite incorporating various drugs and growth factors to enhance cell attachment, cryopreservation to increase the cellular viability upon revival.
32156543	9	9	theme	cell	1343:1346	arg1	systems					1354:1360	cell based systems	1343:1360	cell based systems aimed for increasing cell survival upon cryopreservation and thus has a great potential for bone repair and regeneration	1343:1481	These results validate the potential of our formulated hydrogels as cell based systems aimed for increasing cell survival upon cryopreservation and thus has a great potential for bone repair and regeneration.
32156543	9	9	theme	cell	1343:1346	arg1	potential					1302:1310	the potential	1298:1310	the potential of our formulated hydrogels	1298:1338	These results validate the potential of our formulated hydrogels as cell based systems aimed for increasing cell survival upon cryopreservation and thus has a great potential for bone repair and regeneration.
32156543	7	10	theme	cryopreservation	1031:1046	arg1	30 days					1020:1026	30 days	1020:1026	30 days of cryopreservation	1020:1046	The clonogenic potential of seeded cells upon 30 days of cryopreservation was also evaluated which was 55% in IGF-1 incorporated scaffold cells.
32156543	2	11	theme	sericin	280:286	arg1	composite					303:311	a sericin based hydrogel composite	278:311	a sericin based hydrogel composite incorporating various drugs and growth factors to enhance cell attachment	278:385	In our current study we aimed to develop a sericin based hydrogel composite incorporating various drugs and growth factors to enhance cell attachment, cryopreservation to increase the cellular viability upon revival.
32156543	4	12	theme	higher	626:631	arg1	biocompatibility					633:648	higher biocompatibility	626:648	higher biocompatibility	626:648	The hydrogels prepared were porous and showed higher biocompatibility.
32156543	8	13	theme	flow	1121:1124	arg1	protocol					1151:1158	A flow cytometry based staining protocol	1119:1158	A flow cytometry based staining protocol using Annexin V	1119:1174	A flow cytometry based staining protocol using Annexin V was developed which showed a live cell population up to 80% even after 30 days of crypreservation.
32156543	9	14	theme	cell	1383:1386	arg1	survival					1388:1395	cell survival	1383:1395	cell survival	1383:1395	These results validate the potential of our formulated hydrogels as cell based systems aimed for increasing cell survival upon cryopreservation and thus has a great potential for bone repair and regeneration.
32156543	8	15	theme	based	1136:1140	arg1	protocol					1151:1158	A flow cytometry based staining protocol	1119:1158	A flow cytometry based staining protocol using Annexin V	1119:1174	A flow cytometry based staining protocol using Annexin V was developed which showed a live cell population up to 80% even after 30 days of crypreservation.
32156543	3	16	theme	blended	487:493	arg1	composites					504:513	Sericin, gelatin and carrageenan blended hydrogel composites	454:513	Sericin, gelatin and carrageenan blended hydrogel composites	454:513	Sericin, gelatin and carrageenan blended hydrogel composites were prepared and explored for their physicochemical properties.
32156543	3	17	theme	physicochemical	552:566	arg1	properties					568:577	their physicochemical properties	546:577	their physicochemical properties	546:577	Sericin, gelatin and carrageenan blended hydrogel composites were prepared and explored for their physicochemical properties.
32156543	7	18	theme	clonogenic	978:987	arg1	potential					989:997	The clonogenic potential	974:997	The clonogenic potential of seeded cells upon 30 days of cryopreservation	974:1046	The clonogenic potential of seeded cells upon 30 days of cryopreservation was also evaluated which was 55% in IGF-1 incorporated scaffold cells.
32156543	1	19	theme	major	178:182	arg1	challenge					184:192	a major challenge	176:192	a major challenge in cell based bone regeneration therapies	176:234	Cryopreservation and the low revival rate of cryopreserved cells remains a major challenge in cell based bone regeneration therapies.
32156543	8	20	theme	cytometry	1126:1134	arg1	protocol					1151:1158	A flow cytometry based staining protocol	1119:1158	A flow cytometry based staining protocol using Annexin V	1119:1174	A flow cytometry based staining protocol using Annexin V was developed which showed a live cell population up to 80% even after 30 days of crypreservation.
32156543	6	21	theme	incorporated	844:855	arg1	composites					867:876	IGF-1 incorporated hydrogels composites	838:876	IGF-1 incorporated hydrogels composites	838:876	IGF-1 incorporated hydrogels composites showed better osteogenic cell attachment, proliferation and cell revival upon cryopreservation.
32156543	6	22	dep	incorporated	844:855	arg1	IGF-1					838:842	IGF-1	838:842	IGF-1	838:842	IGF-1 incorporated hydrogels composites showed better osteogenic cell attachment, proliferation and cell revival upon cryopreservation.
32156543	0	23	theme	carrageenan	8:18	arg1	composites					37:46	carrageenan sericin hydrogel composites	8:46	Gelatin carrageenan sericin hydrogel composites	0:46	Gelatin carrageenan sericin hydrogel composites improves cell viability of cryopreserved SaOS-2 cells.
32156543	5	24	theme	release	821:827	arg1	profile					829:835	sustained drug release profile	806:835	sustained drug release profile	806:835	Further, silver nanoparticles, alendronate and insulin like growth factor (IGF-1) were incorporated into the hybrid hydrogels individually and checked for sustained drug release profile.
32156543	8	25	theme	cell	1210:1213	arg1	population					1215:1224	a live cell population	1203:1224	a live cell population up to 80%	1203:1234	A flow cytometry based staining protocol using Annexin V was developed which showed a live cell population up to 80% even after 30 days of crypreservation.
32156543	2	26	theme	various	327:333	arg1	drugs					335:339	various drugs	327:339	various drugs	327:339	In our current study we aimed to develop a sericin based hydrogel composite incorporating various drugs and growth factors to enhance cell attachment, cryopreservation to increase the cellular viability upon revival.
32156543	8	27	theme	staining	1142:1149	arg1	protocol					1151:1158	A flow cytometry based staining protocol	1119:1158	A flow cytometry based staining protocol using Annexin V	1119:1174	A flow cytometry based staining protocol using Annexin V was developed which showed a live cell population up to 80% even after 30 days of crypreservation.
32156543	7	28	theme	cells	1009:1013	arg1	potential					989:997	The clonogenic potential	974:997	The clonogenic potential of seeded cells upon 30 days of cryopreservation	974:1046	The clonogenic potential of seeded cells upon 30 days of cryopreservation was also evaluated which was 55% in IGF-1 incorporated scaffold cells.
32156543	1	29	theme	cell	197:200	arg1	therapies					226:234	cell based bone regeneration therapies	197:234	cell based bone regeneration therapies	197:234	Cryopreservation and the low revival rate of cryopreserved cells remains a major challenge in cell based bone regeneration therapies.
32156543	0	30	theme	hydrogel	28:35	arg1	composites					37:46	carrageenan sericin hydrogel composites	8:46	Gelatin carrageenan sericin hydrogel composites	0:46	Gelatin carrageenan sericin hydrogel composites improves cell viability of cryopreserved SaOS-2 cells.
32156543	8	31	theme	Annexin	1166:1172	arg1	V					1174:1174	Annexin V	1166:1174	Annexin V	1166:1174	A flow cytometry based staining protocol using Annexin V was developed which showed a live cell population up to 80% even after 30 days of crypreservation.
32156543	5	32	theme	silver	660:665	arg1	alendronate					682:692	alendronate	682:692	alendronate	682:692	Further, silver nanoparticles, alendronate and insulin like growth factor (IGF-1) were incorporated into the hybrid hydrogels individually and checked for sustained drug release profile.
32156543	5	32	theme	silver	660:665	arg1	nanoparticles					667:679	silver nanoparticles	660:679	silver nanoparticles	660:679	Further, silver nanoparticles, alendronate and insulin like growth factor (IGF-1) were incorporated into the hybrid hydrogels individually and checked for sustained drug release profile.
32156543	5	32	theme	silver	660:665	arg1	insulin					698:704	insulin	698:704	insulin	698:704	Further, silver nanoparticles, alendronate and insulin like growth factor (IGF-1) were incorporated into the hybrid hydrogels individually and checked for sustained drug release profile.
32156543	1	33	theme	based	202:206	arg1	therapies					226:234	cell based bone regeneration therapies	197:234	cell based bone regeneration therapies	197:234	Cryopreservation and the low revival rate of cryopreserved cells remains a major challenge in cell based bone regeneration therapies.
32156543	0	34	theme	sericin	20:26	arg1	composites					37:46	carrageenan sericin hydrogel composites	8:46	Gelatin carrageenan sericin hydrogel composites	0:46	Gelatin carrageenan sericin hydrogel composites improves cell viability of cryopreserved SaOS-2 cells.
32156543	2	35	theme	hydrogel	294:301	arg1	composite					303:311	a sericin based hydrogel composite	278:311	a sericin based hydrogel composite incorporating various drugs and growth factors to enhance cell attachment	278:385	In our current study we aimed to develop a sericin based hydrogel composite incorporating various drugs and growth factors to enhance cell attachment, cryopreservation to increase the cellular viability upon revival.
32156543	1	36	theme	bone	208:211	arg1	therapies					226:234	cell based bone regeneration therapies	197:234	cell based bone regeneration therapies	197:234	Cryopreservation and the low revival rate of cryopreserved cells remains a major challenge in cell based bone regeneration therapies.
32156543	2	37	theme	growth	345:350	arg1	factors					352:358	growth factors	345:358	growth factors	345:358	In our current study we aimed to develop a sericin based hydrogel composite incorporating various drugs and growth factors to enhance cell attachment, cryopreservation to increase the cellular viability upon revival.
32156543	2	38	theme	cellular	421:428	arg1	viability					430:438	the cellular viability	417:438	the cellular viability	417:438	In our current study we aimed to develop a sericin based hydrogel composite incorporating various drugs and growth factors to enhance cell attachment, cryopreservation to increase the cellular viability upon revival.
32156543	2	39	theme	current	244:250	arg1	study					252:256	our current study	240:256	our current study	240:256	In our current study we aimed to develop a sericin based hydrogel composite incorporating various drugs and growth factors to enhance cell attachment, cryopreservation to increase the cellular viability upon revival.
32156543	7	40	from	%	1079:1079	arg1	cells					1112:1116	IGF-1 incorporated scaffold cells	1084:1116	IGF-1 incorporated scaffold cells	1084:1116	The clonogenic potential of seeded cells upon 30 days of cryopreservation was also evaluated which was 55% in IGF-1 incorporated scaffold cells.
32156543	1	41	theme	regeneration	213:224	arg1	therapies					226:234	cell based bone regeneration therapies	197:234	cell based bone regeneration therapies	197:234	Cryopreservation and the low revival rate of cryopreserved cells remains a major challenge in cell based bone regeneration therapies.
32156543	3	42	theme	Sericin	454:460	arg1	composites					504:513	Sericin, gelatin and carrageenan blended hydrogel composites	454:513	Sericin, gelatin and carrageenan blended hydrogel composites	454:513	Sericin, gelatin and carrageenan blended hydrogel composites were prepared and explored for their physicochemical properties.
32156543	5	43	theme	hybrid	760:765	arg1	hydrogels					767:775	the hybrid hydrogels	756:775	the hybrid hydrogels	756:775	Further, silver nanoparticles, alendronate and insulin like growth factor (IGF-1) were incorporated into the hybrid hydrogels individually and checked for sustained drug release profile.
32156543	8	44	theme	crypreservation	1258:1272	arg1	30 days					1247:1253	30 days	1247:1253	30 days of crypreservation	1247:1272	A flow cytometry based staining protocol using Annexin V was developed which showed a live cell population up to 80% even after 30 days of crypreservation.
32156543	7	45	theme	incorporated	1090:1101	arg1	cells					1112:1116	IGF-1 incorporated scaffold cells	1084:1116	IGF-1 incorporated scaffold cells	1084:1116	The clonogenic potential of seeded cells upon 30 days of cryopreservation was also evaluated which was 55% in IGF-1 incorporated scaffold cells.
32156543	8	46	dep	80	1232:1233	arg1	to					1229:1230	to	1229:1230	to	1229:1230	A flow cytometry based staining protocol using Annexin V was developed which showed a live cell population up to 80% even after 30 days of crypreservation.
32156543	9	47	theme	great	1434:1438	arg1	potential					1440:1448	a great potential	1432:1448	a great potential for bone repair and regeneration	1432:1481	These results validate the potential of our formulated hydrogels as cell based systems aimed for increasing cell survival upon cryopreservation and thus has a great potential for bone repair and regeneration.
32156543	8	48	theme	live	1205:1208	arg1	population					1215:1224	a live cell population	1203:1224	a live cell population up to 80%	1203:1234	A flow cytometry based staining protocol using Annexin V was developed which showed a live cell population up to 80% even after 30 days of crypreservation.
32156543	7	49	theme	scaffold	1103:1110	arg1	cells					1112:1116	IGF-1 incorporated scaffold cells	1084:1116	IGF-1 incorporated scaffold cells	1084:1116	The clonogenic potential of seeded cells upon 30 days of cryopreservation was also evaluated which was 55% in IGF-1 incorporated scaffold cells.
32156543	1	50	theme	low	128:130	arg1	rate					140:143	the low revival rate	124:143	the low revival rate	124:143	Cryopreservation and the low revival rate of cryopreserved cells remains a major challenge in cell based bone regeneration therapies.
32156543	0	51	theme	cell	57:60	arg1	viability					62:70	cell viability	57:70	cell viability of cryopreserved SaOS-2 cells	57:100	Gelatin carrageenan sericin hydrogel composites improves cell viability of cryopreserved SaOS-2 cells.
32156543	9	52	theme	formulated	1319:1328	arg1	hydrogels					1330:1338	our formulated hydrogels	1315:1338	our formulated hydrogels	1315:1338	These results validate the potential of our formulated hydrogels as cell based systems aimed for increasing cell survival upon cryopreservation and thus has a great potential for bone repair and regeneration.
32156543	8	53	dep	population	1215:1224	arg1	%					1234:1234	%	1234:1234	%	1234:1234	A flow cytometry based staining protocol using Annexin V was developed which showed a live cell population up to 80% even after 30 days of crypreservation.
32156543	1	54	theme	revival	132:138	arg1	rate					140:143	the low revival rate	124:143	the low revival rate	124:143	Cryopreservation and the low revival rate of cryopreserved cells remains a major challenge in cell based bone regeneration therapies.
32156543	9	55	theme	hydrogels	1330:1338	arg1	potential					1302:1310	the potential	1298:1310	the potential of our formulated hydrogels	1298:1338	These results validate the potential of our formulated hydrogels as cell based systems aimed for increasing cell survival upon cryopreservation and thus has a great potential for bone repair and regeneration.
32156543	9	55	theme	hydrogels	1330:1338	arg1	systems					1354:1360	cell based systems	1343:1360	cell based systems aimed for increasing cell survival upon cryopreservation and thus has a great potential for bone repair and regeneration	1343:1481	These results validate the potential of our formulated hydrogels as cell based systems aimed for increasing cell survival upon cryopreservation and thus has a great potential for bone repair and regeneration.
32156543	7	56	theme	IGF-1	1084:1088	arg1	cells					1112:1116	IGF-1 incorporated scaffold cells	1084:1116	IGF-1 incorporated scaffold cells	1084:1116	The clonogenic potential of seeded cells upon 30 days of cryopreservation was also evaluated which was 55% in IGF-1 incorporated scaffold cells.
32156543	3	57	theme	hydrogel	495:502	arg1	composites					504:513	Sericin, gelatin and carrageenan blended hydrogel composites	454:513	Sericin, gelatin and carrageenan blended hydrogel composites	454:513	Sericin, gelatin and carrageenan blended hydrogel composites were prepared and explored for their physicochemical properties.
32156543	7	58	theme	seeded	1002:1007	arg1	cells					1009:1013	seeded cells	1002:1013	seeded cells	1002:1013	The clonogenic potential of seeded cells upon 30 days of cryopreservation was also evaluated which was 55% in IGF-1 incorporated scaffold cells.
32156543	9	59	theme	bone	1454:1457	arg1	repair					1459:1464	bone repair	1454:1464	bone repair	1454:1464	These results validate the potential of our formulated hydrogels as cell based systems aimed for increasing cell survival upon cryopreservation and thus has a great potential for bone repair and regeneration.
32156543	6	60	theme	cell	903:906	arg1	attachment					908:917	better osteogenic cell attachment	885:917	better osteogenic cell attachment	885:917	IGF-1 incorporated hydrogels composites showed better osteogenic cell attachment, proliferation and cell revival upon cryopreservation.
32156543	1	61	theme	cells	162:166	arg1	rate					140:143	the low revival rate	124:143	the low revival rate	124:143	Cryopreservation and the low revival rate of cryopreserved cells remains a major challenge in cell based bone regeneration therapies.
32156543	1	61	theme	cells	162:166	arg1	Cryopreservation					103:118	Cryopreservation	103:118	Cryopreservation	103:118	Cryopreservation and the low revival rate of cryopreserved cells remains a major challenge in cell based bone regeneration therapies.
32156543	6	62	theme	hydrogels	857:865	arg1	composites					867:876	IGF-1 incorporated hydrogels composites	838:876	IGF-1 incorporated hydrogels composites	838:876	IGF-1 incorporated hydrogels composites showed better osteogenic cell attachment, proliferation and cell revival upon cryopreservation.
32156543	0	63	theme	cryopreserved	75:87	arg1	cells					96:100	cryopreserved SaOS-2 cells	75:100	cryopreserved SaOS-2 cells	75:100	Gelatin carrageenan sericin hydrogel composites improves cell viability of cryopreserved SaOS-2 cells.
32156543	6	64	theme	osteogenic	892:901	arg1	attachment					908:917	better osteogenic cell attachment	885:917	better osteogenic cell attachment	885:917	IGF-1 incorporated hydrogels composites showed better osteogenic cell attachment, proliferation and cell revival upon cryopreservation.
32156543	3	65	theme	gelatin	463:469	arg1	composites					504:513	Sericin, gelatin and carrageenan blended hydrogel composites	454:513	Sericin, gelatin and carrageenan blended hydrogel composites	454:513	Sericin, gelatin and carrageenan blended hydrogel composites were prepared and explored for their physicochemical properties.
32156543	3	66	theme	carrageenan	475:485	arg1	composites					504:513	Sericin, gelatin and carrageenan blended hydrogel composites	454:513	Sericin, gelatin and carrageenan blended hydrogel composites	454:513	Sericin, gelatin and carrageenan blended hydrogel composites were prepared and explored for their physicochemical properties.
32156543	1	67	theme	cryopreserved	148:160	arg1	cells					162:166	cryopreserved cells	148:166	cryopreserved cells	148:166	Cryopreservation and the low revival rate of cryopreserved cells remains a major challenge in cell based bone regeneration therapies.
32156543	5	68	theme	sustained	806:814	arg1	profile					829:835	sustained drug release profile	806:835	sustained drug release profile	806:835	Further, silver nanoparticles, alendronate and insulin like growth factor (IGF-1) were incorporated into the hybrid hydrogels individually and checked for sustained drug release profile.
32156543	9	69	theme	based	1348:1352	arg1	systems					1354:1360	cell based systems	1343:1360	cell based systems aimed for increasing cell survival upon cryopreservation and thus has a great potential for bone repair and regeneration	1343:1481	These results validate the potential of our formulated hydrogels as cell based systems aimed for increasing cell survival upon cryopreservation and thus has a great potential for bone repair and regeneration.
32156543	9	69	theme	based	1348:1352	arg1	potential					1302:1310	the potential	1298:1310	the potential of our formulated hydrogels	1298:1338	These results validate the potential of our formulated hydrogels as cell based systems aimed for increasing cell survival upon cryopreservation and thus has a great potential for bone repair and regeneration.
32156543	0	70	dep	Gelatin	0:6	arg1	composites					37:46	carrageenan sericin hydrogel composites	8:46	Gelatin carrageenan sericin hydrogel composites	0:46	Gelatin carrageenan sericin hydrogel composites improves cell viability of cryopreserved SaOS-2 cells.
32156543	2	71	theme	cell	371:374	arg1	attachment					376:385	cell attachment	371:385	cell attachment	371:385	In our current study we aimed to develop a sericin based hydrogel composite incorporating various drugs and growth factors to enhance cell attachment, cryopreservation to increase the cellular viability upon revival.
32489097	2	0	with	reactions	223:231	arg1	7					238:238	7	238:238	7	238:238	Glycosylation reactions with 7 gave >80% yields, and only β-xylulofuranosides were isolated in all cases.
32489097	3	1	theme	enterocolitica	500:513	arg1	O:5/O:5,27					524:533	Yersinia enterocolitica serovars O:5/O:5,27	491:533	Yersinia enterocolitica serovars O:5/O:5,27	491:533	The utility of 7 for the synthesis of complex glycans was shown by its successful application to the preparation of the repeating unit from the lipopolysaccharide O-antigen of Yersinia enterocolitica serovars O:5/O:5,27.
32489097	4	2	theme	β-xylulofuranose	581:596	arg1	residues					598:605	two β-xylulofuranose residues	577:605	two β-xylulofuranose residues; using 7, both were introduced simultaneously with excellent stereocontrol	577:680	This structure is a pentasaccharide with two β-xylulofuranose residues; using 7, both were introduced simultaneously with excellent stereocontrol.
32489097	3	3	theme	Yersinia	491:498	arg1	enterocolitica					500:513	Yersinia enterocolitica	491:513	Yersinia enterocolitica serovars O:5/O:5,27	491:533	The utility of 7 for the synthesis of complex glycans was shown by its successful application to the preparation of the repeating unit from the lipopolysaccharide O-antigen of Yersinia enterocolitica serovars O:5/O:5,27.
32489097	4	4	with	pentasaccharide	556:570	arg1	residues					598:605	two β-xylulofuranose residues	577:605	two β-xylulofuranose residues; using 7, both were introduced simultaneously with excellent stereocontrol	577:680	This structure is a pentasaccharide with two β-xylulofuranose residues; using 7, both were introduced simultaneously with excellent stereocontrol.
32489097	2	5	theme	only	262:265	arg1	β-xylulofuranosides					267:285	only β-xylulofuranosides	262:285	only β-xylulofuranosides	262:285	Glycosylation reactions with 7 gave >80% yields, and only β-xylulofuranosides were isolated in all cases.
32489097	0	6	theme	Siloxane-Bridged	2:17	arg1	Donor					28:32	A Siloxane-Bridged Glycosyl Donor	0:32	A Siloxane-Bridged Glycosyl Donor	0:32	A Siloxane-Bridged Glycosyl Donor Enables Highly Stereoselective β-Xylulofuranosylation.
32489097	1	7	theme	siloxane-protected	101:118	arg1	donor					120:124	a siloxane-protected donor	99:124	a siloxane-protected donor (7) for the highly stereoselective formation of β-(2,3-cis)-xylulofuranosyl bonds	99:206	We report a siloxane-protected donor (7) for the highly stereoselective formation of β-(2,3-cis)-xylulofuranosyl bonds.
32489097	1	7	theme	siloxane-protected	101:118	arg1	7					127:127	7	127:127	7	127:127	We report a siloxane-protected donor (7) for the highly stereoselective formation of β-(2,3-cis)-xylulofuranosyl bonds.
32489097	3	8	from	O-antigen	478:486	arg1	preparation					416:426	the preparation	412:426	the preparation of the repeating unit from the lipopolysaccharide O-antigen	412:486	The utility of 7 for the synthesis of complex glycans was shown by its successful application to the preparation of the repeating unit from the lipopolysaccharide O-antigen of Yersinia enterocolitica serovars O:5/O:5,27.
32489097	1	9	theme	-xylulofuranosyl	185:200	arg1	bonds					202:206	β-(2,3-cis)-xylulofuranosyl bonds	174:206	β-(2,3-cis)-xylulofuranosyl bonds	174:206	We report a siloxane-protected donor (7) for the highly stereoselective formation of β-(2,3-cis)-xylulofuranosyl bonds.
32489097	3	10	theme	complex	353:359	arg1	glycans					361:367	complex glycans	353:367	complex glycans	353:367	The utility of 7 for the synthesis of complex glycans was shown by its successful application to the preparation of the repeating unit from the lipopolysaccharide O-antigen of Yersinia enterocolitica serovars O:5/O:5,27.
32489097	3	11	theme	unit	445:448	arg1	preparation					416:426	the preparation	412:426	the preparation of the repeating unit from the lipopolysaccharide O-antigen	412:486	The utility of 7 for the synthesis of complex glycans was shown by its successful application to the preparation of the repeating unit from the lipopolysaccharide O-antigen of Yersinia enterocolitica serovars O:5/O:5,27.
32489097	3	12	theme	glycans	361:367	arg1	synthesis					340:348	the synthesis	336:348	the synthesis of complex glycans	336:367	The utility of 7 for the synthesis of complex glycans was shown by its successful application to the preparation of the repeating unit from the lipopolysaccharide O-antigen of Yersinia enterocolitica serovars O:5/O:5,27.
32489097	1	13	theme	stereoselective	145:159	arg1	formation					161:169	the highly stereoselective formation	134:169	the highly stereoselective formation of β-(2,3-cis)-xylulofuranosyl bonds	134:206	We report a siloxane-protected donor (7) for the highly stereoselective formation of β-(2,3-cis)-xylulofuranosyl bonds.
32489097	3	14	theme	successful	386:395	arg1	application					397:407	its successful application	382:407	its successful application to the preparation of the repeating unit from the lipopolysaccharide O-antigen of Yersinia enterocolitica serovars O:5/O:5,27	382:533	The utility of 7 for the synthesis of complex glycans was shown by its successful application to the preparation of the repeating unit from the lipopolysaccharide O-antigen of Yersinia enterocolitica serovars O:5/O:5,27.
32489097	2	15	theme	>80	245:247	arg1	%					248:248	%	248:248	%	248:248	Glycosylation reactions with 7 gave >80% yields, and only β-xylulofuranosides were isolated in all cases.
32489097	4	16	theme	excellent	658:666	arg1	stereocontrol					668:680	excellent stereocontrol	658:680	excellent stereocontrol	658:680	This structure is a pentasaccharide with two β-xylulofuranose residues; using 7, both were introduced simultaneously with excellent stereocontrol.
32489097	0	17	theme	Glycosyl	19:26	arg1	Donor					28:32	A Siloxane-Bridged Glycosyl Donor	0:32	A Siloxane-Bridged Glycosyl Donor	0:32	A Siloxane-Bridged Glycosyl Donor Enables Highly Stereoselective β-Xylulofuranosylation.
32489097	4	18	dep	residues	598:605	arg1	introduced					627:636	introduced	627:636	were introduced simultaneously with excellent stereocontrol	622:680	This structure is a pentasaccharide with two β-xylulofuranose residues; using 7, both were introduced simultaneously with excellent stereocontrol.
32489097	3	19	theme	repeating	435:443	arg1	unit					445:448	the repeating unit	431:448	the repeating unit	431:448	The utility of 7 for the synthesis of complex glycans was shown by its successful application to the preparation of the repeating unit from the lipopolysaccharide O-antigen of Yersinia enterocolitica serovars O:5/O:5,27.
32489097	0	20	theme	Stereoselective	49:63	arg1	β-Xylulofuranosylation					65:86	Highly Stereoselective β-Xylulofuranosylation	42:86	Highly Stereoselective β-Xylulofuranosylation	42:86	A Siloxane-Bridged Glycosyl Donor Enables Highly Stereoselective β-Xylulofuranosylation.
32489097	2	21	theme	Glycosylation	209:221	arg1	reactions					223:231	Glycosylation reactions	209:231	Glycosylation reactions with 7	209:238	Glycosylation reactions with 7 gave >80% yields, and only β-xylulofuranosides were isolated in all cases.
32489097	3	22	theme	lipopolysaccharide	459:476	arg1	O-antigen					478:486	the lipopolysaccharide O-antigen	455:486	the lipopolysaccharide O-antigen	455:486	The utility of 7 for the synthesis of complex glycans was shown by its successful application to the preparation of the repeating unit from the lipopolysaccharide O-antigen of Yersinia enterocolitica serovars O:5/O:5,27.
32489097	1	23	theme	bonds	202:206	arg1	formation					161:169	the highly stereoselective formation	134:169	the highly stereoselective formation of β-(2,3-cis)-xylulofuranosyl bonds	134:206	We report a siloxane-protected donor (7) for the highly stereoselective formation of β-(2,3-cis)-xylulofuranosyl bonds.
32489097	3	24	theme	serovars	515:522	arg1	O:5/O:5,27					524:533	Yersinia enterocolitica serovars O:5/O:5,27	491:533	Yersinia enterocolitica serovars O:5/O:5,27	491:533	The utility of 7 for the synthesis of complex glycans was shown by its successful application to the preparation of the repeating unit from the lipopolysaccharide O-antigen of Yersinia enterocolitica serovars O:5/O:5,27.
32489097	3	25	theme	7	330:330	arg1	utility					319:325	The utility	315:325	The utility of 7 for the synthesis of complex glycans	315:367	The utility of 7 for the synthesis of complex glycans was shown by its successful application to the preparation of the repeating unit from the lipopolysaccharide O-antigen of Yersinia enterocolitica serovars O:5/O:5,27.
32489097	2	26	theme	%	248:248	arg1	yields					250:255	>80% yields	245:255	>80% yields	245:255	Glycosylation reactions with 7 gave >80% yields, and only β-xylulofuranosides were isolated in all cases.
32489097	3	27	theme	O:5/O:5,27	524:533	arg1	application					397:407	its successful application	382:407	its successful application to the preparation of the repeating unit from the lipopolysaccharide O-antigen of Yersinia enterocolitica serovars O:5/O:5,27	382:533	The utility of 7 for the synthesis of complex glycans was shown by its successful application to the preparation of the repeating unit from the lipopolysaccharide O-antigen of Yersinia enterocolitica serovars O:5/O:5,27.
31907709	4	0	theme	SA	960:961	arg1	type					925:928	salt type	920:928	salt type	920:928	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	4	0	theme	SA	960:961	arg1	degree					950:955	esterification degree	935:955	esterification degree	935:955	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	4	0	theme	SA	960:961	arg1	viscosity					898:906	viscosity	898:906	viscosity	898:906	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	4	0	theme	SA	960:961	arg1	content					911:917	G content	909:917	G content	909:917	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	3	1	theme	structure	681:689	arg1	viscosity					713:721	viscosity	713:721	viscosity	713:721	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	1	theme	structure	681:689	arg1	ratio					728:732	G/M ratio	724:732	G/M ratio	724:732	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	1	theme	structure	681:689	arg1	type					743:746	SA salt type	735:746	SA salt type	735:746	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	1	theme	structure	681:689	arg1	degree					753:758	degree	753:758	degree of esterification	753:776	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	1	theme	structure	681:689	arg1	properties					691:700	SA structure properties	678:700	SA structure properties	678:700	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	2	theme	drug	517:520	arg1	system					531:536	PEC-based sustained drug delivery system	497:536	PEC-based sustained drug delivery system	497:536	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	6	3	with	thickness	1308:1316	arg1	extent					1354:1359	different swelling and erosion extent	1323:1359	different swelling and erosion extent	1323:1359	Potassium, magnesium salt of SA, and esterified SA can help form PEC of higher thickness with different swelling and erosion extent.
31907709	2	4	theme	previous	272:279	arg1	studies					281:287	Our previous studies	268:287	Our previous studies	268:287	Our previous studies found that chitosan (CS)- and alginate (SA)-based tablets can form self-assembled polyelectrolyte complex (PEC) film on the surface, which controlled drug release with a novel mechanism.
31907709	1	5	theme	sustained	175:183	arg1	tablets					193:199	matrix-based sustained release tablets	162:199	matrix-based sustained release tablets	162:199	Natural polymers are promising as the carrier of matrix-based sustained release tablets but limited by their diversity in source and structure properties.
31907709	1	6	theme	tablets	193:199	arg1	carrier					151:157	the carrier	147:157	the carrier of matrix-based sustained release tablets	147:199	Natural polymers are promising as the carrier of matrix-based sustained release tablets but limited by their diversity in source and structure properties.
31907709	7	7	theme	CS-SA	1492:1496	arg1	strategy					1539:1546	a promising strategy	1527:1546	a promising strategy to manufacture natural polymer-based products with constant quality	1527:1614	In conclusion, this study demonstrated that drug release diversity due to SA structure difference can be well eradicated by using CS-SA combination system, which is a promising strategy to manufacture natural polymer-based products with constant quality.
31907709	7	7	theme	CS-SA	1492:1496	arg1	system					1510:1515	CS-SA combination system	1492:1515	CS-SA combination system	1492:1515	In conclusion, this study demonstrated that drug release diversity due to SA structure difference can be well eradicated by using CS-SA combination system, which is a promising strategy to manufacture natural polymer-based products with constant quality.
31907709	3	8	theme	single-matrix	568:580	arg1	diversity					591:599	single-matrix material diversity	568:599	single-matrix material diversity	568:599	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	9	from	effect	668:673	arg1	profiles					794:801	drug release profiles	781:801	drug release profiles	781:801	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	10	theme	G/M	724:726	arg1	ratio					728:732	G/M ratio	724:732	G/M ratio	724:732	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	0	11	from	Tablets	104:110	arg1	Release					39:45	Drug Release	34:45	Drug Release from Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets	34:110	Effect of Sodium Alginate Type on Drug Release from Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets.
31907709	5	12	from	system	1221:1226	arg1	release					1190:1196	drug release	1185:1196	drug release from CS-SA combination system	1185:1226	SA of low viscosity is superior in controlling drug release from CS-SA combination system.
31907709	4	13	theme	parameters	1069:1078	arg1	effect					1053:1058	little effect	1046:1058	little effect of these parameters on drug release	1046:1094	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	4	14	dep	viscosity	898:906	arg1	the					894:896	the	894:896	the	894:896	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	3	15	theme	drug	604:607	arg1	behavior					617:624	drug release behavior	604:624	drug release behavior	604:624	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	16	from	influence	555:563	arg1	behavior					617:624	drug release behavior	604:624	drug release behavior	604:624	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	17	theme	drug	781:784	arg1	profiles					794:801	drug release profiles	781:801	drug release profiles	781:801	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	2	18	theme	novel	459:463	arg1	mechanism					465:473	a novel mechanism	457:473	a novel mechanism	457:473	Our previous studies found that chitosan (CS)- and alginate (SA)-based tablets can form self-assembled polyelectrolyte complex (PEC) film on the surface, which controlled drug release with a novel mechanism.
31907709	3	19	theme	salt	738:741	arg1	type					743:746	SA salt type	735:746	SA salt type	735:746	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	20	theme	diversity	591:599	arg1	influence					555:563	the influence	551:563	the influence of single-matrix material diversity on drug release behavior	551:624	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	7	21	theme	structure	1439:1447	arg1	difference					1449:1458	SA structure difference	1436:1458	SA structure difference	1436:1458	In conclusion, this study demonstrated that drug release diversity due to SA structure difference can be well eradicated by using CS-SA combination system, which is a promising strategy to manufacture natural polymer-based products with constant quality.
31907709	2	22	from	film	401:404	arg1	surface					413:419	the surface	409:419	the surface	409:419	Our previous studies found that chitosan (CS)- and alginate (SA)-based tablets can form self-assembled polyelectrolyte complex (PEC) film on the surface, which controlled drug release with a novel mechanism.
31907709	4	23	theme	little	1046:1051	arg1	effect					1053:1058	little effect	1046:1058	little effect of these parameters on drug release	1046:1094	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	3	24	theme	esterification	763:776	arg1	viscosity					713:721	viscosity	713:721	viscosity	713:721	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	24	theme	esterification	763:776	arg1	ratio					728:732	G/M ratio	724:732	G/M ratio	724:732	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	24	theme	esterification	763:776	arg1	type					743:746	SA salt type	735:746	SA salt type	735:746	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	24	theme	esterification	763:776	arg1	degree					753:758	degree	753:758	degree of esterification	753:776	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	0	25	theme	Alginate-Based	68:81	arg1	Tablets					104:110	Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets	52:110	Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets	52:110	Effect of Sodium Alginate Type on Drug Release from Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets.
31907709	7	26	theme	natural	1563:1569	arg1	products					1585:1592	natural polymer-based products	1563:1592	natural polymer-based products	1563:1592	In conclusion, this study demonstrated that drug release diversity due to SA structure difference can be well eradicated by using CS-SA combination system, which is a promising strategy to manufacture natural polymer-based products with constant quality.
31907709	0	27	from	Effect	0:5	arg1	Release					39:45	Drug Release	34:45	Drug Release from Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets	34:110	Effect of Sodium Alginate Type on Drug Release from Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets.
31907709	5	28	theme	low	1144:1146	arg1	viscosity					1148:1156	low viscosity	1144:1156	low viscosity	1144:1156	SA of low viscosity is superior in controlling drug release from CS-SA combination system.
31907709	6	29	theme	magnesium	1240:1248	arg1	salt					1250:1253	magnesium salt	1240:1253	magnesium salt of SA	1240:1259	Potassium, magnesium salt of SA, and esterified SA can help form PEC of higher thickness with different swelling and erosion extent.
31907709	4	30	contain	had	963:965	arg1	type					925:928	salt type	920:928	salt type	920:928	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	4	30	contain	had	963:965	arg1	degree					950:955	esterification degree	935:955	esterification degree	935:955	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	4	30	contain	had	963:965	arg2	influence					980:988	a remarkable influence	967:988	a remarkable influence on drug release	967:1004	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	4	30	contain	had	963:965	arg1	viscosity					898:906	viscosity	898:906	viscosity	898:906	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	4	30	contain	had	963:965	arg1	content					911:917	G content	909:917	G content	909:917	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	3	31	theme	composite	835:843	arg1	system					845:850	CS-SA composite system	829:850	CS-SA composite system	829:850	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	4	32	from	influence	980:988	arg1	release					998:1004	drug release	993:1004	drug release	993:1004	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	4	33	theme	combination	1118:1128	arg1	system					1130:1135	CS-SA combination system	1112:1135	CS-SA combination system	1112:1135	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	5	34	theme	combination	1209:1219	arg1	system					1221:1226	CS-SA combination system	1203:1226	CS-SA combination system	1203:1226	SA of low viscosity is superior in controlling drug release from CS-SA combination system.
31907709	2	35	theme	polyelectrolyte	371:385	arg1	film					401:404	self-assembled polyelectrolyte complex (PEC) film	356:404	self-assembled polyelectrolyte complex (PEC) film on the surface, which controlled drug release with a novel mechanism	356:473	Our previous studies found that chitosan (CS)- and alginate (SA)-based tablets can form self-assembled polyelectrolyte complex (PEC) film on the surface, which controlled drug release with a novel mechanism.
31907709	2	36	theme	drug	439:442	arg1	release					444:450	drug release	439:450	drug release with a novel mechanism	439:473	Our previous studies found that chitosan (CS)- and alginate (SA)-based tablets can form self-assembled polyelectrolyte complex (PEC) film on the surface, which controlled drug release with a novel mechanism.
31907709	7	37	theme	promising	1529:1537	arg1	strategy					1539:1546	a promising strategy	1527:1546	a promising strategy to manufacture natural polymer-based products with constant quality	1527:1614	In conclusion, this study demonstrated that drug release diversity due to SA structure difference can be well eradicated by using CS-SA combination system, which is a promising strategy to manufacture natural polymer-based products with constant quality.
31907709	7	37	theme	promising	1529:1537	arg1	system					1510:1515	CS-SA combination system	1492:1515	CS-SA combination system	1492:1515	In conclusion, this study demonstrated that drug release diversity due to SA structure difference can be well eradicated by using CS-SA combination system, which is a promising strategy to manufacture natural polymer-based products with constant quality.
31907709	4	38	theme	esterification	935:948	arg1	degree					950:955	esterification degree	935:955	esterification degree	935:955	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	4	39	theme	drug	1083:1086	arg1	release					1088:1094	drug release	1083:1094	drug release	1083:1094	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	5	40	theme	drug	1185:1188	arg1	release					1190:1196	drug release	1185:1196	drug release from CS-SA combination system	1185:1226	SA of low viscosity is superior in controlling drug release from CS-SA combination system.
31907709	0	41	theme	Sodium	10:15	arg1	Type					26:29	Sodium Alginate Type	10:29	Sodium Alginate Type	10:29	Effect of Sodium Alginate Type on Drug Release from Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets.
31907709	4	42	theme	salt	920:923	arg1	type					925:928	salt type	920:928	salt type	920:928	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	0	43	theme	Type	26:29	arg1	Effect					0:5	Effect	0:5	Effect of Sodium Alginate Type on Drug Release from Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets.	0:111	Effect of Sodium Alginate Type on Drug Release from Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets.
31907709	6	44	theme	esterified	1266:1275	arg1	SA					1277:1278	esterified SA	1266:1278	esterified SA	1266:1278	Potassium, magnesium salt of SA, and esterified SA can help form PEC of higher thickness with different swelling and erosion extent.
31907709	3	45	theme	drug	652:655	arg1	model					657:661	a drug model	650:661	a drug model	650:661	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	45	theme	drug	652:655	arg1	effect					668:673	the effect	664:673	the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles	664:801	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	45	theme	drug	652:655	arg1	theophylline					634:645	theophylline	634:645	theophylline	634:645	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	45	theme	drug	652:655	arg1	swelling					804:811	swelling	804:811	swelling	804:811	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	46	theme	PEC-based	497:505	arg1	system					531:536	PEC-based sustained drug delivery system	497:536	PEC-based sustained drug delivery system	497:536	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	6	47	theme	SA	1258:1259	arg1	Potassium					1229:1237	Potassium	1229:1237	Potassium	1229:1237	Potassium, magnesium salt of SA, and esterified SA can help form PEC of higher thickness with different swelling and erosion extent.
31907709	6	47	theme	SA	1258:1259	arg1	salt					1250:1253	magnesium salt	1240:1253	magnesium salt of SA	1240:1259	Potassium, magnesium salt of SA, and esterified SA can help form PEC of higher thickness with different swelling and erosion extent.
31907709	6	47	theme	SA	1258:1259	arg1	SA					1277:1278	esterified SA	1266:1278	esterified SA	1266:1278	Potassium, magnesium salt of SA, and esterified SA can help form PEC of higher thickness with different swelling and erosion extent.
31907709	6	48	theme	different	1323:1331	arg1	extent					1354:1359	different swelling and erosion extent	1323:1359	different swelling and erosion extent	1323:1359	Potassium, magnesium salt of SA, and esterified SA can help form PEC of higher thickness with different swelling and erosion extent.
31907709	4	49	theme	remarkable	969:978	arg1	influence					980:988	a remarkable influence	967:988	a remarkable influence on drug release	967:1004	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	3	50	theme	sustained	507:515	arg1	system					531:536	PEC-based sustained drug delivery system	497:536	PEC-based sustained drug delivery system	497:536	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	6	51	theme	thickness	1308:1316	arg1	PEC					1294:1296	PEC	1294:1296	PEC of higher thickness with different swelling and erosion extent	1294:1359	Potassium, magnesium salt of SA, and esterified SA can help form PEC of higher thickness with different swelling and erosion extent.
31907709	7	52	theme	constant	1599:1606	arg1	quality					1608:1614	constant quality	1599:1614	constant quality	1599:1614	In conclusion, this study demonstrated that drug release diversity due to SA structure difference can be well eradicated by using CS-SA combination system, which is a promising strategy to manufacture natural polymer-based products with constant quality.
31907709	3	53	theme	properties	691:700	arg1	model					657:661	a drug model	650:661	a drug model	650:661	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	53	theme	properties	691:700	arg1	effect					668:673	the effect	664:673	the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles	664:801	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	1	54	theme	matrix-based	162:173	arg1	tablets					193:199	matrix-based sustained release tablets	162:199	matrix-based sustained release tablets	162:199	Natural polymers are promising as the carrier of matrix-based sustained release tablets but limited by their diversity in source and structure properties.
31907709	0	55	theme	Drug	34:37	arg1	Release					39:45	Drug Release	34:45	Drug Release from Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets	34:110	Effect of Sodium Alginate Type on Drug Release from Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets.
31907709	3	56	theme	SA	678:679	arg1	viscosity					713:721	viscosity	713:721	viscosity	713:721	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	56	theme	SA	678:679	arg1	ratio					728:732	G/M ratio	724:732	G/M ratio	724:732	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	56	theme	SA	678:679	arg1	type					743:746	SA salt type	735:746	SA salt type	735:746	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	56	theme	SA	678:679	arg1	degree					753:758	degree	753:758	degree of esterification	753:776	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	56	theme	SA	678:679	arg1	properties					691:700	SA structure properties	678:700	SA structure properties	678:700	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	1	57	theme	release	185:191	arg1	tablets					193:199	matrix-based sustained release tablets	162:199	matrix-based sustained release tablets	162:199	Natural polymers are promising as the carrier of matrix-based sustained release tablets but limited by their diversity in source and structure properties.
31907709	7	58	theme	combination	1498:1508	arg1	strategy					1539:1546	a promising strategy	1527:1546	a promising strategy to manufacture natural polymer-based products with constant quality	1527:1614	In conclusion, this study demonstrated that drug release diversity due to SA structure difference can be well eradicated by using CS-SA combination system, which is a promising strategy to manufacture natural polymer-based products with constant quality.
31907709	7	58	theme	combination	1498:1508	arg1	system					1510:1515	CS-SA combination system	1492:1515	CS-SA combination system	1492:1515	In conclusion, this study demonstrated that drug release diversity due to SA structure difference can be well eradicated by using CS-SA combination system, which is a promising strategy to manufacture natural polymer-based products with constant quality.
31907709	3	59	theme	delivery	522:529	arg1	system					531:536	PEC-based sustained drug delivery system	497:536	PEC-based sustained drug delivery system	497:536	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	1	60	dep	source	235:240	arg1	properties					256:265	properties	256:265	properties	256:265	Natural polymers are promising as the carrier of matrix-based sustained release tablets but limited by their diversity in source and structure properties.
31907709	3	61	theme	SA	735:736	arg1	type					743:746	SA salt type	735:746	SA salt type	735:746	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	1	62	theme	Natural	113:119	arg1	polymers					121:128	Natural polymers	113:128	Natural polymers	113:128	Natural polymers are promising as the carrier of matrix-based sustained release tablets but limited by their diversity in source and structure properties.
31907709	5	63	theme	viscosity	1148:1156	arg1	SA					1138:1139	SA	1138:1139	SA of low viscosity	1138:1156	SA of low viscosity is superior in controlling drug release from CS-SA combination system.
31907709	1	64	from	diversity	222:230	arg1	source					235:240	source	235:240	source	235:240	Natural polymers are promising as the carrier of matrix-based sustained release tablets but limited by their diversity in source and structure properties.
31907709	1	64	from	diversity	222:230	arg1	structure					246:254	structure	246:254	structure	246:254	Natural polymers are promising as the carrier of matrix-based sustained release tablets but limited by their diversity in source and structure properties.
31907709	0	65	theme	Chitosan-Sodium	52:66	arg1	Tablets					104:110	Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets	52:110	Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets	52:110	Effect of Sodium Alginate Type on Drug Release from Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets.
31907709	3	66	theme	material	582:589	arg1	diversity					591:599	single-matrix material diversity	568:599	single-matrix material diversity	568:599	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	3	67	theme	release	786:792	arg1	profiles					794:801	drug release profiles	781:801	drug release profiles	781:801	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	7	68	theme	SA	1436:1437	arg1	difference					1449:1458	SA structure difference	1436:1458	SA structure difference	1436:1458	In conclusion, this study demonstrated that drug release diversity due to SA structure difference can be well eradicated by using CS-SA combination system, which is a promising strategy to manufacture natural polymer-based products with constant quality.
31907709	0	69	theme	In	83:84	arg1	Tablets					104:110	Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets	52:110	Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets	52:110	Effect of Sodium Alginate Type on Drug Release from Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets.
31907709	4	70	theme	G	909:909	arg1	content					911:917	G content	909:917	G content	909:917	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	4	71	theme	CS-SA	1112:1116	arg1	system					1130:1135	CS-SA combination system	1112:1135	CS-SA combination system	1112:1135	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	0	72	theme	Film-Forming	91:102	arg1	Tablets					104:110	Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets	52:110	Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets	52:110	Effect of Sodium Alginate Type on Drug Release from Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets.
31907709	2	73	with	release	444:450	arg1	mechanism					465:473	a novel mechanism	457:473	a novel mechanism	457:473	Our previous studies found that chitosan (CS)- and alginate (SA)-based tablets can form self-assembled polyelectrolyte complex (PEC) film on the surface, which controlled drug release with a novel mechanism.
31907709	7	74	theme	polymer-based	1571:1583	arg1	products					1585:1592	natural polymer-based products	1563:1592	natural polymer-based products	1563:1592	In conclusion, this study demonstrated that drug release diversity due to SA structure difference can be well eradicated by using CS-SA combination system, which is a promising strategy to manufacture natural polymer-based products with constant quality.
31907709	7	75	theme	release	1411:1417	arg1	diversity					1419:1427	drug release diversity	1406:1427	drug release diversity due to SA structure difference	1406:1458	In conclusion, this study demonstrated that drug release diversity due to SA structure difference can be well eradicated by using CS-SA combination system, which is a promising strategy to manufacture natural polymer-based products with constant quality.
31907709	5	76	theme	CS-SA	1203:1207	arg1	system					1221:1226	CS-SA combination system	1203:1226	CS-SA combination system	1203:1226	SA of low viscosity is superior in controlling drug release from CS-SA combination system.
31907709	3	77	theme	system	845:850	arg1	erosion					818:824	erosion	818:824	erosion of CS-SA composite system	818:850	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	2	78	dep	-based	332:337	arg1	alginate					319:326	alginate	319:326	alginate	319:326	Our previous studies found that chitosan (CS)- and alginate (SA)-based tablets can form self-assembled polyelectrolyte complex (PEC) film on the surface, which controlled drug release with a novel mechanism.
31907709	2	78	dep	-based	332:337	arg1	CS					310:311	CS	310:311	CS	310:311	Our previous studies found that chitosan (CS)- and alginate (SA)-based tablets can form self-assembled polyelectrolyte complex (PEC) film on the surface, which controlled drug release with a novel mechanism.
31907709	2	78	dep	-based	332:337	arg1	chitosan					300:307	chitosan	300:307	chitosan	300:307	Our previous studies found that chitosan (CS)- and alginate (SA)-based tablets can form self-assembled polyelectrolyte complex (PEC) film on the surface, which controlled drug release with a novel mechanism.
31907709	2	79	theme	self-assembled	356:369	arg1	film					401:404	self-assembled polyelectrolyte complex (PEC) film	356:404	self-assembled polyelectrolyte complex (PEC) film on the surface, which controlled drug release with a novel mechanism	356:473	Our previous studies found that chitosan (CS)- and alginate (SA)-based tablets can form self-assembled polyelectrolyte complex (PEC) film on the surface, which controlled drug release with a novel mechanism.
31907709	6	80	theme	higher	1301:1306	arg1	thickness					1308:1316	higher thickness	1301:1316	higher thickness with different swelling and erosion extent	1301:1359	Potassium, magnesium salt of SA, and esterified SA can help form PEC of higher thickness with different swelling and erosion extent.
31907709	0	81	dep	In	83:84	arg1	Situ					86:89	Situ	86:89	Situ	86:89	Effect of Sodium Alginate Type on Drug Release from Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets.
31907709	3	82	theme	CS-SA	829:833	arg1	system					845:850	CS-SA composite system	829:850	CS-SA composite system	829:850	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	0	83	theme	Alginate	17:24	arg1	Type					26:29	Sodium Alginate Type	10:29	Sodium Alginate Type	10:29	Effect of Sodium Alginate Type on Drug Release from Chitosan-Sodium Alginate-Based In Situ Film-Forming Tablets.
31907709	2	84	theme	PEC	396:398	arg1	film					401:404	self-assembled polyelectrolyte complex (PEC) film	356:404	self-assembled polyelectrolyte complex (PEC) film on the surface, which controlled drug release with a novel mechanism	356:473	Our previous studies found that chitosan (CS)- and alginate (SA)-based tablets can form self-assembled polyelectrolyte complex (PEC) film on the surface, which controlled drug release with a novel mechanism.
31907709	7	85	theme	drug	1406:1409	arg1	diversity					1419:1427	drug release diversity	1406:1427	drug release diversity due to SA structure difference	1406:1458	In conclusion, this study demonstrated that drug release diversity due to SA structure difference can be well eradicated by using CS-SA combination system, which is a promising strategy to manufacture natural polymer-based products with constant quality.
31907709	3	86	theme	release	609:615	arg1	behavior					617:624	drug release behavior	604:624	drug release behavior	604:624	To elucidate whether PEC-based sustained drug delivery system could weaken the influence of single-matrix material diversity on drug release behavior, taking theophylline as a drug model, the effect of SA structure properties, including viscosity, G/M ratio, SA salt type, and degree of esterification on drug release profiles, swelling, and erosion of CS-SA composite system was investigated.
31907709	2	87	theme	complex	387:393	arg1	film					401:404	self-assembled polyelectrolyte complex (PEC) film	356:404	self-assembled polyelectrolyte complex (PEC) film on the surface, which controlled drug release with a novel mechanism	356:473	Our previous studies found that chitosan (CS)- and alginate (SA)-based tablets can form self-assembled polyelectrolyte complex (PEC) film on the surface, which controlled drug release with a novel mechanism.
31907709	4	88	used	used	1024:1027	arg2	matrix					1034:1039	a matrix	1032:1039	a matrix	1032:1039	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	4	88	used	used	1024:1027	arg2	SA					1011:1012	SA	1011:1012	SA alone	1011:1018	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	4	89	located	observed	1100:1107	arg2	effect					1053:1058	little effect	1046:1058	little effect of these parameters on drug release	1046:1094	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	4	89	located	observed	1100:1107	arg1	system					1130:1135	CS-SA combination system	1112:1135	CS-SA combination system	1112:1135	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	4	90	from	effect	1053:1058	arg1	release					1088:1094	drug release	1083:1094	drug release	1083:1094	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	6	91	theme	erosion	1346:1352	arg1	extent					1354:1359	different swelling and erosion extent	1323:1359	different swelling and erosion extent	1323:1359	Potassium, magnesium salt of SA, and esterified SA can help form PEC of higher thickness with different swelling and erosion extent.
31907709	4	92	theme	drug	993:996	arg1	release					998:1004	drug release	993:1004	drug release	993:1004	The results showed that the viscosity, G content, salt type, and esterification degree of SA had a remarkable influence on drug release when SA alone was used as a matrix, but little effect of these parameters on drug release was observed in CS-SA combination system.
31907709	2	93	theme	-based	332:337	arg1	tablets					339:345	chitosan (CS)- and alginate (SA)-based tablets	300:345	chitosan (CS)- and alginate (SA)-based tablets	300:345	Our previous studies found that chitosan (CS)- and alginate (SA)-based tablets can form self-assembled polyelectrolyte complex (PEC) film on the surface, which controlled drug release with a novel mechanism.
31907709	6	94	theme	swelling	1333:1340	arg1	extent					1354:1359	different swelling and erosion extent	1323:1359	different swelling and erosion extent	1323:1359	Potassium, magnesium salt of SA, and esterified SA can help form PEC of higher thickness with different swelling and erosion extent.
32761978	0	0	theme	dressing	95:102	arg1	applications					104:115	skin wound dressing applications	84:115	skin wound dressing applications	84:115	Novel functional antimicrobial and biocompatible arabinoxylan/guar gum hydrogel for skin wound dressing applications.
32761978	11	1	theme	potential	1659:1667	arg1	material					1669:1676	a potential material	1657:1676	a potential material for wound dressing application	1657:1707	It is, therefore, believed that as-developed hydrogel could be a potential material for wound dressing application.
32761978	11	1	theme	potential	1659:1667	arg1	hydrogel					1639:1646	as-developed hydrogel	1626:1646	as-developed hydrogel	1626:1646	It is, therefore, believed that as-developed hydrogel could be a potential material for wound dressing application.
32761978	7	2	theme	Degradability	1070:1082	arg1	tests					1084:1088	Degradability tests	1070:1088	Degradability tests	1070:1088	Degradability tests showed that hydrogels lost maximum weight in 7 days.
32761978	1	3	theme	biomacromolecular	161:177	arg1	materials					194:202	active biomacromolecular wound dressing materials	154:202	active biomacromolecular wound dressing materials that are biocompatible	154:225	It is a challenging task to develop active biomacromolecular wound dressing materials that are biocompatible and possesses antibacterial properties against the bacterial strains that cause severe skin disease.
32761978	0	4	theme	wound	89:93	arg1	applications					104:115	skin wound dressing applications	84:115	skin wound dressing applications	84:115	Novel functional antimicrobial and biocompatible arabinoxylan/guar gum hydrogel for skin wound dressing applications.
32761978	3	5	theme	orthosilicate	700:712	arg1	addition					665:672	the addition	661:672	the addition of crosslinker tetraethyl orthosilicate	661:712	Fourier transform infrared spectroscopy (FT-IR) confirmed that both ARX and GG interacted well with each other, and their interactions further increased with the addition of crosslinker tetraethyl orthosilicate.
32761978	1	6	theme	wound	179:183	arg1	materials					194:202	active biomacromolecular wound dressing materials	154:202	active biomacromolecular wound dressing materials that are biocompatible	154:225	It is a challenging task to develop active biomacromolecular wound dressing materials that are biocompatible and possesses antibacterial properties against the bacterial strains that cause severe skin disease.
32761978	10	7	theme	normal	1575:1580	arg1	activities					1582:1591	their normal activities	1569:1591	their normal activities	1569:1591	The cells not only retained their cylindrical morphologies onto the hydrogel but were also performing their normal activities.
32761978	9	8	dep	aeruginosa	1312:1321	arg1	Gram-negative					1324:1336	Gram-negative	1324:1336	Gram-negative	1324:1336	The hydrogel samples showed good antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) in PBS, good drug release profile (92% drug release), and nontoxic cellular behavior.
32761978	8	9	theme	pH.	1193:1195	arg1	swelling					1205:1212	pH. Maximum swelling	1193:1212	pH. Maximum swelling	1193:1212	All the samples showed variation in swelling with pH. Maximum swelling was observed at pH 7.
32761978	10	10	dep	cells	1471:1475	arg1	only					1481:1484	only	1481:1484	only	1481:1484	The cells not only retained their cylindrical morphologies onto the hydrogel but were also performing their normal activities.
32761978	4	11	theme	uniform	769:775	arg1	morphologies					784:795	uniform porous morphologies	769:795	uniform porous morphologies of the hydrogels	769:812	Scanning electron microscope (SEM) micrographs showed uniform porous morphologies of the hydrogels.
32761978	11	12	theme	wound	1682:1686	arg1	application					1697:1707	wound dressing application	1682:1707	wound dressing application	1682:1707	It is, therefore, believed that as-developed hydrogel could be a potential material for wound dressing application.
32761978	4	13	theme	electron	724:731	arg1	SEM					745:747	SEM	745:747	SEM	745:747	Scanning electron microscope (SEM) micrographs showed uniform porous morphologies of the hydrogels.
32761978	4	13	theme	electron	724:731	arg1	microscope					733:742	Scanning electron microscope	715:742	Scanning electron microscope (SEM) micrographs	715:760	Scanning electron microscope (SEM) micrographs showed uniform porous morphologies of the hydrogels.
32761978	1	14	theme	severe	307:312	arg1	disease					319:325	severe skin disease	307:325	severe skin disease	307:325	It is a challenging task to develop active biomacromolecular wound dressing materials that are biocompatible and possesses antibacterial properties against the bacterial strains that cause severe skin disease.
32761978	4	15	theme	Scanning	715:722	arg1	SEM					745:747	SEM	745:747	SEM	745:747	Scanning electron microscope (SEM) micrographs showed uniform porous morphologies of the hydrogels.
32761978	4	15	theme	Scanning	715:722	arg1	microscope					733:742	Scanning electron microscope	715:742	Scanning electron microscope (SEM) micrographs	715:760	Scanning electron microscope (SEM) micrographs showed uniform porous morphologies of the hydrogels.
32761978	6	16	theme	Elastic	934:940	arg1	modulus					942:948	Elastic modulus	934:948	Elastic modulus	934:948	Elastic modulus, tensile strength, and fracture strain of the hydrogels significantly improved (from ATG-1 to ATG-4) with crosslinking.
32761978	11	17	theme	dressing	1688:1695	arg1	application					1697:1707	wound dressing application	1682:1707	wound dressing application	1682:1707	It is, therefore, believed that as-developed hydrogel could be a potential material for wound dressing application.
32761978	3	18	dep	Fourier	503:509	arg1	transform					511:519	transform	511:519	transform infrared spectroscopy (FT-IR)	511:549	Fourier transform infrared spectroscopy (FT-IR) confirmed that both ARX and GG interacted well with each other, and their interactions further increased with the addition of crosslinker tetraethyl orthosilicate.
32761978	1	19	theme	dressing	185:192	arg1	materials					194:202	active biomacromolecular wound dressing materials	154:202	active biomacromolecular wound dressing materials that are biocompatible	154:225	It is a challenging task to develop active biomacromolecular wound dressing materials that are biocompatible and possesses antibacterial properties against the bacterial strains that cause severe skin disease.
32761978	8	20	theme	Maximum	1197:1203	arg1	swelling					1205:1212	pH. Maximum swelling	1193:1212	pH. Maximum swelling	1193:1212	All the samples showed variation in swelling with pH. Maximum swelling was observed at pH 7.
32761978	2	21	theme	natural	485:491	arg1	polymers					493:500	natural polymers	485:500	natural polymers	485:500	This work is focused on the preparation of a biocompatible and degradable hydrogel for wound dressing application using arabinoxylan (ARX) and guar gum (GG) natural polymers.
32761978	7	22	theme	maximum	1117:1123	arg1	weight					1125:1130	maximum weight	1117:1130	maximum weight	1117:1130	Degradability tests showed that hydrogels lost maximum weight in 7 days.
32761978	2	23	theme	guar	471:474	arg1	GG					481:482	GG	481:482	GG	481:482	This work is focused on the preparation of a biocompatible and degradable hydrogel for wound dressing application using arabinoxylan (ARX) and guar gum (GG) natural polymers.
32761978	2	23	theme	guar	471:474	arg1	gum					476:478	guar gum	471:478	guar gum (GG)	471:483	This work is focused on the preparation of a biocompatible and degradable hydrogel for wound dressing application using arabinoxylan (ARX) and guar gum (GG) natural polymers.
32761978	9	24	theme	release	1399:1405	arg1	release					1425:1431	92% drug release	1416:1431	92% drug release	1416:1431	The hydrogel samples showed good antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) in PBS, good drug release profile (92% drug release), and nontoxic cellular behavior.
32761978	9	24	theme	release	1399:1405	arg1	profile					1407:1413	good drug release profile	1389:1413	good drug release profile (92% drug release)	1389:1432	The hydrogel samples showed good antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) in PBS, good drug release profile (92% drug release), and nontoxic cellular behavior.
32761978	2	25	theme	dressing	421:428	arg1	application					430:440	wound dressing application	415:440	wound dressing application using arabinoxylan (ARX) and guar gum (GG) natural polymers	415:500	This work is focused on the preparation of a biocompatible and degradable hydrogel for wound dressing application using arabinoxylan (ARX) and guar gum (GG) natural polymers.
32761978	0	26	theme	functional	6:15	arg1	antimicrobial					17:29	Novel functional antimicrobial	0:29	Novel functional antimicrobial	0:29	Novel functional antimicrobial and biocompatible arabinoxylan/guar gum hydrogel for skin wound dressing applications.
32761978	6	27	theme	tensile	951:957	arg1	strength					959:966	tensile strength	951:966	tensile strength	951:966	Elastic modulus, tensile strength, and fracture strain of the hydrogels significantly improved (from ATG-1 to ATG-4) with crosslinking.
32761978	6	28	dep	ATG-4	1044:1048	arg1	ATG-1					1035:1039	ATG-1	1035:1039	ATG-1	1035:1039	Elastic modulus, tensile strength, and fracture strain of the hydrogels significantly improved (from ATG-1 to ATG-4) with crosslinking.
32761978	2	29	theme	wound	415:419	arg1	application					430:440	wound dressing application	415:440	wound dressing application using arabinoxylan (ARX) and guar gum (GG) natural polymers	415:500	This work is focused on the preparation of a biocompatible and degradable hydrogel for wound dressing application using arabinoxylan (ARX) and guar gum (GG) natural polymers.
32761978	0	30	theme	Novel	0:4	arg1	antimicrobial					17:29	Novel functional antimicrobial	0:29	Novel functional antimicrobial	0:29	Novel functional antimicrobial and biocompatible arabinoxylan/guar gum hydrogel for skin wound dressing applications.
32761978	1	31	theme	skin	314:317	arg1	disease					319:325	severe skin disease	307:325	severe skin disease	307:325	It is a challenging task to develop active biomacromolecular wound dressing materials that are biocompatible and possesses antibacterial properties against the bacterial strains that cause severe skin disease.
32761978	5	32	theme	increased	894:902	arg1	crosslinking					904:915	the increased crosslinking	890:915	the increased crosslinking of the hydrogel	890:931	The porous morphologies and uniform interconnected pores are attributed to the increased crosslinking of the hydrogel.
32761978	5	33	theme	interconnected	851:864	arg1	pores					866:870	uniform interconnected pores	843:870	uniform interconnected pores	843:870	The porous morphologies and uniform interconnected pores are attributed to the increased crosslinking of the hydrogel.
32761978	5	34	theme	uniform	843:849	arg1	pores					866:870	uniform interconnected pores	843:870	uniform interconnected pores	843:870	The porous morphologies and uniform interconnected pores are attributed to the increased crosslinking of the hydrogel.
32761978	9	35	theme	hydrogel	1240:1247	arg1	samples					1249:1255	The hydrogel samples	1236:1255	The hydrogel samples	1236:1255	The hydrogel samples showed good antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) in PBS, good drug release profile (92% drug release), and nontoxic cellular behavior.
32761978	4	36	theme	porous	777:782	arg1	morphologies					784:795	uniform porous morphologies	769:795	uniform porous morphologies of the hydrogels	769:812	Scanning electron microscope (SEM) micrographs showed uniform porous morphologies of the hydrogels.
32761978	6	37	theme	hydrogels	996:1004	arg1	strain					982:987	fracture strain	973:987	fracture strain	973:987	Elastic modulus, tensile strength, and fracture strain of the hydrogels significantly improved (from ATG-1 to ATG-4) with crosslinking.
32761978	6	37	theme	hydrogels	996:1004	arg1	modulus					942:948	Elastic modulus	934:948	Elastic modulus	934:948	Elastic modulus, tensile strength, and fracture strain of the hydrogels significantly improved (from ATG-1 to ATG-4) with crosslinking.
32761978	6	37	theme	hydrogels	996:1004	arg1	strength					959:966	tensile strength	951:966	tensile strength	951:966	Elastic modulus, tensile strength, and fracture strain of the hydrogels significantly improved (from ATG-1 to ATG-4) with crosslinking.
32761978	4	38	theme	hydrogels	804:812	arg1	morphologies					784:795	uniform porous morphologies	769:795	uniform porous morphologies of the hydrogels	769:812	Scanning electron microscope (SEM) micrographs showed uniform porous morphologies of the hydrogels.
32761978	2	39	theme	biocompatible	373:385	arg1	hydrogel					402:409	a biocompatible and degradable hydrogel	371:409	a biocompatible and degradable hydrogel for wound dressing application using arabinoxylan (ARX) and guar gum (GG) natural polymers	371:500	This work is focused on the preparation of a biocompatible and degradable hydrogel for wound dressing application using arabinoxylan (ARX) and guar gum (GG) natural polymers.
32761978	5	40	theme	hydrogel	924:931	arg1	crosslinking					904:915	the increased crosslinking	890:915	the increased crosslinking of the hydrogel	890:931	The porous morphologies and uniform interconnected pores are attributed to the increased crosslinking of the hydrogel.
32761978	0	41	theme	arabinoxylan/guar	49:65	arg1	hydrogel					71:78	biocompatible arabinoxylan/guar gum hydrogel	35:78	biocompatible arabinoxylan/guar gum hydrogel	35:78	Novel functional antimicrobial and biocompatible arabinoxylan/guar gum hydrogel for skin wound dressing applications.
32761978	4	42	theme	microscope	733:742	arg1	micrographs					750:760	Scanning electron microscope (SEM) micrographs	715:760	Scanning electron microscope (SEM) micrographs	715:760	Scanning electron microscope (SEM) micrographs showed uniform porous morphologies of the hydrogels.
32761978	2	43	dep	arabinoxylan	448:459	arg1	polymers					493:500	natural polymers	485:500	natural polymers	485:500	This work is focused on the preparation of a biocompatible and degradable hydrogel for wound dressing application using arabinoxylan (ARX) and guar gum (GG) natural polymers.
32761978	0	44	theme	biocompatible	35:47	arg1	hydrogel					71:78	biocompatible arabinoxylan/guar gum hydrogel	35:78	biocompatible arabinoxylan/guar gum hydrogel	35:78	Novel functional antimicrobial and biocompatible arabinoxylan/guar gum hydrogel for skin wound dressing applications.
32761978	9	45	theme	good	1389:1392	arg1	release					1425:1431	92% drug release	1416:1431	92% drug release	1416:1431	The hydrogel samples showed good antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) in PBS, good drug release profile (92% drug release), and nontoxic cellular behavior.
32761978	9	45	theme	good	1389:1392	arg1	profile					1407:1413	good drug release profile	1389:1413	good drug release profile (92% drug release)	1389:1432	The hydrogel samples showed good antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) in PBS, good drug release profile (92% drug release), and nontoxic cellular behavior.
32761978	9	46	theme	good	1264:1267	arg1	activity					1283:1290	good antibacterial activity	1264:1290	good antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) in PBS	1264:1386	The hydrogel samples showed good antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) in PBS, good drug release profile (92% drug release), and nontoxic cellular behavior.
32761978	8	47	located	observed	1218:1225	arg2	swelling					1205:1212	pH. Maximum swelling	1193:1212	pH. Maximum swelling	1193:1212	All the samples showed variation in swelling with pH. Maximum swelling was observed at pH 7.
32761978	8	47	located	observed	1218:1225	arg1	pH					1230:1231	pH 7	1230:1233	pH 7	1230:1233	All the samples showed variation in swelling with pH. Maximum swelling was observed at pH 7.
32761978	1	48	theme	antibacterial	241:253	arg1	properties					255:264	antibacterial properties	241:264	antibacterial properties	241:264	It is a challenging task to develop active biomacromolecular wound dressing materials that are biocompatible and possesses antibacterial properties against the bacterial strains that cause severe skin disease.
32761978	5	49	theme	porous	819:824	arg1	morphologies					826:837	The porous morphologies	815:837	The porous morphologies	815:837	The porous morphologies and uniform interconnected pores are attributed to the increased crosslinking of the hydrogel.
32761978	9	50	theme	drug	1394:1397	arg1	release					1425:1431	92% drug release	1416:1431	92% drug release	1416:1431	The hydrogel samples showed good antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) in PBS, good drug release profile (92% drug release), and nontoxic cellular behavior.
32761978	9	50	theme	drug	1394:1397	arg1	profile					1407:1413	good drug release profile	1389:1413	good drug release profile (92% drug release)	1389:1432	The hydrogel samples showed good antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) in PBS, good drug release profile (92% drug release), and nontoxic cellular behavior.
32761978	9	51	theme	antibacterial	1269:1281	arg1	activity					1283:1290	good antibacterial activity	1264:1290	good antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) in PBS	1264:1386	The hydrogel samples showed good antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) in PBS, good drug release profile (92% drug release), and nontoxic cellular behavior.
32761978	9	52	theme	nontoxic	1439:1446	arg1	behavior					1457:1464	nontoxic cellular behavior	1439:1464	nontoxic cellular behavior	1439:1464	The hydrogel samples showed good antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) in PBS, good drug release profile (92% drug release), and nontoxic cellular behavior.
32761978	0	53	theme	gum	67:69	arg1	hydrogel					71:78	biocompatible arabinoxylan/guar gum hydrogel	35:78	biocompatible arabinoxylan/guar gum hydrogel	35:78	Novel functional antimicrobial and biocompatible arabinoxylan/guar gum hydrogel for skin wound dressing applications.
32761978	6	54	theme	fracture	973:980	arg1	strain					982:987	fracture strain	973:987	fracture strain	973:987	Elastic modulus, tensile strength, and fracture strain of the hydrogels significantly improved (from ATG-1 to ATG-4) with crosslinking.
32761978	3	55	theme	crosslinker	677:687	arg1	orthosilicate					700:712	crosslinker tetraethyl orthosilicate	677:712	crosslinker tetraethyl orthosilicate	677:712	Fourier transform infrared spectroscopy (FT-IR) confirmed that both ARX and GG interacted well with each other, and their interactions further increased with the addition of crosslinker tetraethyl orthosilicate.
32761978	3	56	theme	tetraethyl	689:698	arg1	orthosilicate					700:712	crosslinker tetraethyl orthosilicate	677:712	crosslinker tetraethyl orthosilicate	677:712	Fourier transform infrared spectroscopy (FT-IR) confirmed that both ARX and GG interacted well with each other, and their interactions further increased with the addition of crosslinker tetraethyl orthosilicate.
32761978	1	57	theme	challenging	126:136	arg1	task					138:141	a challenging task	124:141	a challenging task	124:141	It is a challenging task to develop active biomacromolecular wound dressing materials that are biocompatible and possesses antibacterial properties against the bacterial strains that cause severe skin disease.
32761978	1	57	theme	challenging	126:136	arg1	It					118:119	It	118:119	It	118:119	It is a challenging task to develop active biomacromolecular wound dressing materials that are biocompatible and possesses antibacterial properties against the bacterial strains that cause severe skin disease.
32761978	1	58	contain	possesses	231:239	arg1	task					138:141	a challenging task	124:141	a challenging task	124:141	It is a challenging task to develop active biomacromolecular wound dressing materials that are biocompatible and possesses antibacterial properties against the bacterial strains that cause severe skin disease.
32761978	1	58	contain	possesses	231:239	arg2	properties					255:264	antibacterial properties	241:264	antibacterial properties	241:264	It is a challenging task to develop active biomacromolecular wound dressing materials that are biocompatible and possesses antibacterial properties against the bacterial strains that cause severe skin disease.
32761978	1	58	contain	possesses	231:239	arg1	It					118:119	It	118:119	It	118:119	It is a challenging task to develop active biomacromolecular wound dressing materials that are biocompatible and possesses antibacterial properties against the bacterial strains that cause severe skin disease.
32761978	10	59	theme	cylindrical	1501:1511	arg1	morphologies					1513:1524	their cylindrical morphologies	1495:1524	their cylindrical morphologies	1495:1524	The cells not only retained their cylindrical morphologies onto the hydrogel but were also performing their normal activities.
32761978	3	60	dep	transform	511:519	arg1	infrared					521:528	infrared	521:528	transform infrared spectroscopy (FT-IR)	511:549	Fourier transform infrared spectroscopy (FT-IR) confirmed that both ARX and GG interacted well with each other, and their interactions further increased with the addition of crosslinker tetraethyl orthosilicate.
32761978	6	61	dep	improved	1020:1027	arg1	ATG-4					1044:1048	ATG-4	1044:1048	ATG-4	1044:1048	Elastic modulus, tensile strength, and fracture strain of the hydrogels significantly improved (from ATG-1 to ATG-4) with crosslinking.
32761978	11	62	theme	as-developed	1626:1637	arg1	material					1669:1676	a potential material	1657:1676	a potential material for wound dressing application	1657:1707	It is, therefore, believed that as-developed hydrogel could be a potential material for wound dressing application.
32761978	11	62	theme	as-developed	1626:1637	arg1	hydrogel					1639:1646	as-developed hydrogel	1626:1646	as-developed hydrogel	1626:1646	It is, therefore, believed that as-developed hydrogel could be a potential material for wound dressing application.
32761978	2	63	theme	degradable	391:400	arg1	hydrogel					402:409	a biocompatible and degradable hydrogel	371:409	a biocompatible and degradable hydrogel for wound dressing application using arabinoxylan (ARX) and guar gum (GG) natural polymers	371:500	This work is focused on the preparation of a biocompatible and degradable hydrogel for wound dressing application using arabinoxylan (ARX) and guar gum (GG) natural polymers.
32761978	0	64	theme	skin	84:87	arg1	applications					104:115	skin wound dressing applications	84:115	skin wound dressing applications	84:115	Novel functional antimicrobial and biocompatible arabinoxylan/guar gum hydrogel for skin wound dressing applications.
32761978	2	65	theme	hydrogel	402:409	arg1	preparation					356:366	the preparation	352:366	the preparation of a biocompatible and degradable hydrogel for wound dressing application using arabinoxylan (ARX) and guar gum (GG) natural polymers	352:500	This work is focused on the preparation of a biocompatible and degradable hydrogel for wound dressing application using arabinoxylan (ARX) and guar gum (GG) natural polymers.
32761978	9	66	theme	cellular	1448:1455	arg1	behavior					1457:1464	nontoxic cellular behavior	1439:1464	nontoxic cellular behavior	1439:1464	The hydrogel samples showed good antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) in PBS, good drug release profile (92% drug release), and nontoxic cellular behavior.
32761978	9	67	theme	%	1418:1418	arg1	release					1425:1431	92% drug release	1416:1431	92% drug release	1416:1431	The hydrogel samples showed good antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) in PBS, good drug release profile (92% drug release), and nontoxic cellular behavior.
32761978	9	67	theme	%	1418:1418	arg1	profile					1407:1413	good drug release profile	1389:1413	good drug release profile (92% drug release)	1389:1432	The hydrogel samples showed good antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) in PBS, good drug release profile (92% drug release), and nontoxic cellular behavior.
32761978	9	68	theme	drug	1420:1423	arg1	release					1425:1431	92% drug release	1416:1431	92% drug release	1416:1431	The hydrogel samples showed good antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) in PBS, good drug release profile (92% drug release), and nontoxic cellular behavior.
32761978	9	68	theme	drug	1420:1423	arg1	profile					1407:1413	good drug release profile	1389:1413	good drug release profile (92% drug release)	1389:1432	The hydrogel samples showed good antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) in PBS, good drug release profile (92% drug release), and nontoxic cellular behavior.
32761978	1	69	theme	active	154:159	arg1	materials					194:202	active biomacromolecular wound dressing materials	154:202	active biomacromolecular wound dressing materials that are biocompatible	154:225	It is a challenging task to develop active biomacromolecular wound dressing materials that are biocompatible and possesses antibacterial properties against the bacterial strains that cause severe skin disease.
32761978	1	70	theme	bacterial	278:286	arg1	strains					288:294	the bacterial strains	274:294	the bacterial strains that cause severe skin disease	274:325	It is a challenging task to develop active biomacromolecular wound dressing materials that are biocompatible and possesses antibacterial properties against the bacterial strains that cause severe skin disease.
32761702	0	0	theme	anti-inflammation	107:123	arg1	activity					125:132	its anti-inflammation activity	103:132	its anti-inflammation activity	103:132	Schisandra chinensis ameliorates depressive-like behaviors by regulating microbiota-gut-brain axis via its anti-inflammation activity.
32761702	3	1	theme	tail	462:465	arg1	TST					484:486	TST	484:486	TST	484:486	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	1	theme	tail	462:465	arg1	test					447:450	The forced swimming test	427:450	The forced swimming test (FST)	427:456	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	1	theme	tail	462:465	arg1	test					478:481	tail suspension test	462:481	tail suspension test (TST)	462:487	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	1	theme	tail	462:465	arg1	models					520:525	predictive animal models	502:525	predictive animal models of antidepressant activity	502:552	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	8	2	theme	alterations	1160:1170	arg1	well					1329:1332	well	1329:1332	well	1329:1332	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	8	2	theme	alterations	1160:1170	arg1	diversity					1121:1129	the decreased diversity	1107:1129	the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice	1107:1310	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	8	3	from	mice	1307:1310	arg1	proportions					1188:1198	the relative proportions	1175:1198	the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice	1175:1310	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	8	4	from	members	1284:1290	arg1	mice					1307:1310	LPS-induced mice	1295:1310	LPS-induced mice	1295:1310	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	3	5	theme	suspension	467:476	arg1	TST					484:486	TST	484:486	TST	484:486	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	5	theme	suspension	467:476	arg1	test					447:450	The forced swimming test	427:450	The forced swimming test (FST)	427:456	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	5	theme	suspension	467:476	arg1	test					478:481	tail suspension test	462:481	tail suspension test (TST)	462:487	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	5	theme	suspension	467:476	arg1	models					520:525	predictive animal models	502:525	predictive animal models of antidepressant activity	502:552	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	8	6	theme	decreased	1111:1119	arg1	well					1329:1332	well	1329:1332	well	1329:1332	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	8	6	theme	decreased	1111:1119	arg1	diversity					1121:1129	the decreased diversity	1107:1129	the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice	1107:1310	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	6	7	theme	histopathological	842:858	arg1	examination					860:870	the histopathological examination	838:870	the histopathological examination of the colon	838:883	This was confirmed by the histopathological examination of the colon.
32761702	3	8	theme	predictive	502:511	arg1	test					447:450	The forced swimming test	427:450	The forced swimming test (FST)	427:456	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	8	theme	predictive	502:511	arg1	test					478:481	tail suspension test	462:481	tail suspension test (TST)	462:487	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	8	theme	predictive	502:511	arg1	models					520:525	predictive animal models	502:525	predictive animal models of antidepressant activity	502:552	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	9	9	theme	S.chinensis	1399:1409	arg1	lignans					1362:1368	lignans	1362:1368	lignans	1362:1368	All results suggested that lignans is the effective fraction of S.chinensis to ameliorating depressive disorders, which its anti-inflammation activity possibly involved in the bidirectional connection between gut microbiota and brain.
32761702	9	9	theme	S.chinensis	1399:1409	arg1	fraction					1387:1394	the effective fraction	1373:1394	the effective fraction of S.chinensis to ameliorating depressive disorders, which its anti-inflammation activity possibly involved in the bidirectional connection between gut microbiota and brain	1373:1567	All results suggested that lignans is the effective fraction of S.chinensis to ameliorating depressive disorders, which its anti-inflammation activity possibly involved in the bidirectional connection between gut microbiota and brain.
32761702	8	10	from	proportions	1188:1198	arg1	mice					1307:1310	LPS-induced mice	1295:1310	LPS-induced mice	1295:1310	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	8	11	theme	members	1239:1245	arg1	proportions					1188:1198	the relative proportions	1175:1198	the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice	1175:1310	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	5	12	dep	cytokines	750:758	arg1	both					760:763	both	760:763	both	760:763	We found that treatment with SCE and SCL could significantly decrease the levels of pro-inflammatory cytokines both in the periphery and central nervous system (CNS).
32761702	5	13	theme	nervous	794:800	arg1	system					802:807	central nervous system	786:807	central nervous system (CNS)	786:813	We found that treatment with SCE and SCL could significantly decrease the levels of pro-inflammatory cytokines both in the periphery and central nervous system (CNS).
32761702	5	13	theme	nervous	794:800	arg1	CNS					810:812	CNS	810:812	CNS	810:812	We found that treatment with SCE and SCL could significantly decrease the levels of pro-inflammatory cytokines both in the periphery and central nervous system (CNS).
32761702	1	14	theme	fractions	216:224	arg1	actions					189:195	the antidepressant actions	170:195	the antidepressant actions of the composition fractions of Schisandra chinensis	170:248	The present study aimed to examine the antidepressant actions of the composition fractions of Schisandra chinensis using LPS-induced mice.
32761702	4	15	theme	immobility	622:631	arg1	duration					610:617	the duration	606:617	the duration of immobility in FST and TST	606:646	Total extracts and lignans significantly decreased the duration of immobility in FST and TST.
32761702	8	16	theme	LPS-induced	1295:1305	arg1	mice					1307:1310	LPS-induced mice	1295:1310	LPS-induced mice	1295:1310	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	9	17	theme	bidirectional	1511:1523	arg1	connection					1525:1534	the bidirectional connection	1507:1534	the bidirectional connection between gut microbiota and brain	1507:1567	All results suggested that lignans is the effective fraction of S.chinensis to ameliorating depressive disorders, which its anti-inflammation activity possibly involved in the bidirectional connection between gut microbiota and brain.
32761702	8	18	theme	intestinal	1134:1143	arg1	microbiota					1145:1154	intestinal microbiota	1134:1154	intestinal microbiota	1134:1154	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	1	19	theme	composition	204:214	arg1	fractions					216:224	the composition fractions	200:224	the composition fractions of Schisandra chinensis	200:248	The present study aimed to examine the antidepressant actions of the composition fractions of Schisandra chinensis using LPS-induced mice.
32761702	8	20	theme	microbiota	1145:1154	arg1	well					1329:1332	well	1329:1332	well	1329:1332	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	8	20	theme	microbiota	1145:1154	arg1	diversity					1121:1129	the decreased diversity	1107:1129	the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice	1107:1310	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	7	21	theme	RT-PCR	890:895	arg1	results					897:903	The RT-PCR results	886:903	The RT-PCR results	886:903	The RT-PCR results demonstrated that SCE and SCL could greatly inhibit the TLR4/NF-κB/IKKα signaling pathway.
32761702	3	22	theme	animal	513:518	arg1	test					447:450	The forced swimming test	427:450	The forced swimming test (FST)	427:456	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	22	theme	animal	513:518	arg1	test					478:481	tail suspension test	462:481	tail suspension test (TST)	462:487	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	22	theme	animal	513:518	arg1	models					520:525	predictive animal models	502:525	predictive animal models of antidepressant activity	502:552	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	23	used	used	494:497	arg2	test					447:450	The forced swimming test	427:450	The forced swimming test (FST)	427:456	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	23	used	used	494:497	arg2	models					520:525	predictive animal models	502:525	predictive animal models of antidepressant activity	502:552	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	23	used	used	494:497	arg2	test					478:481	tail suspension test	462:481	tail suspension test (TST)	462:487	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	23	used	used	494:497	arg2	FST					453:455	FST	453:455	FST	453:455	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	23	used	used	494:497	arg2	TST					484:486	TST	484:486	TST	484:486	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	0	24	theme	Schisandra	0:9	arg1	chinensis					11:19	Schisandra chinensis	0:19	Schisandra chinensis	0:19	Schisandra chinensis ameliorates depressive-like behaviors by regulating microbiota-gut-brain axis via its anti-inflammation activity.
32761702	8	25	theme	Bacteroidetes	1203:1215	arg1	members					1239:1245	Bacteroidetes and Firmicutes phylum members	1203:1245	Bacteroidetes and Firmicutes phylum members	1203:1245	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	4	26	from	duration	610:617	arg1	TST					644:646	TST	644:646	TST	644:646	Total extracts and lignans significantly decreased the duration of immobility in FST and TST.
32761702	4	26	from	duration	610:617	arg1	FST					636:638	FST	636:638	FST	636:638	Total extracts and lignans significantly decreased the duration of immobility in FST and TST.
32761702	0	27	theme	depressive-like	33:47	arg1	behaviors					49:57	depressive-like behaviors	33:57	depressive-like behaviors	33:57	Schisandra chinensis ameliorates depressive-like behaviors by regulating microbiota-gut-brain axis via its anti-inflammation activity.
32761702	8	28	theme	acid	1039:1042	arg1	concentrations					1013:1026	the concentrations	1009:1026	the concentrations of Butyric acid and Propionic acid	1009:1061	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	3	29	theme	swimming	438:445	arg1	models					520:525	predictive animal models	502:525	predictive animal models of antidepressant activity	502:552	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	29	theme	swimming	438:445	arg1	test					478:481	tail suspension test	462:481	tail suspension test (TST)	462:487	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	29	theme	swimming	438:445	arg1	FST					453:455	FST	453:455	FST	453:455	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	29	theme	swimming	438:445	arg1	test					447:450	The forced swimming test	427:450	The forced swimming test (FST)	427:456	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	8	30	theme	Butyric	1031:1037	arg1	acid					1039:1042	Butyric acid	1031:1042	Butyric acid	1031:1042	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	1	31	theme	present	139:145	arg1	study					147:151	The present study	135:151	The present study	135:151	The present study aimed to examine the antidepressant actions of the composition fractions of Schisandra chinensis using LPS-induced mice.
32761702	9	32	theme	gut	1544:1546	arg1	microbiota					1548:1557	gut microbiota	1544:1557	gut microbiota	1544:1557	All results suggested that lignans is the effective fraction of S.chinensis to ameliorating depressive disorders, which its anti-inflammation activity possibly involved in the bidirectional connection between gut microbiota and brain.
32761702	1	33	theme	chinensis	240:248	arg1	fractions					216:224	the composition fractions	200:224	the composition fractions of Schisandra chinensis	200:248	The present study aimed to examine the antidepressant actions of the composition fractions of Schisandra chinensis using LPS-induced mice.
32761702	5	34	with	treatment	663:671	arg1	SCL					686:688	SCL	686:688	SCL	686:688	We found that treatment with SCE and SCL could significantly decrease the levels of pro-inflammatory cytokines both in the periphery and central nervous system (CNS).
32761702	5	34	with	treatment	663:671	arg1	SCE					678:680	SCE	678:680	SCE	678:680	We found that treatment with SCE and SCL could significantly decrease the levels of pro-inflammatory cytokines both in the periphery and central nervous system (CNS).
32761702	8	35	theme	Propionic	1048:1056	arg1	acid					1058:1061	Propionic acid	1048:1061	Propionic acid	1048:1061	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	9	36	theme	anti-inflammation	1459:1475	arg1	activity					1477:1484	its anti-inflammation activity	1455:1484	its anti-inflammation activity	1455:1484	All results suggested that lignans is the effective fraction of S.chinensis to ameliorating depressive disorders, which its anti-inflammation activity possibly involved in the bidirectional connection between gut microbiota and brain.
32761702	3	37	theme	activity	545:552	arg1	test					447:450	The forced swimming test	427:450	The forced swimming test (FST)	427:456	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	37	theme	activity	545:552	arg1	test					478:481	tail suspension test	462:481	tail suspension test (TST)	462:487	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	37	theme	activity	545:552	arg1	models					520:525	predictive animal models	502:525	predictive animal models of antidepressant activity	502:552	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	8	38	theme	Barnesiella	1248:1258	arg1	proportions					1188:1198	the relative proportions	1175:1198	the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice	1175:1310	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	5	39	theme	pro-inflammatory	733:748	arg1	cytokines					750:758	pro-inflammatory cytokines	733:758	pro-inflammatory cytokines	733:758	We found that treatment with SCE and SCL could significantly decrease the levels of pro-inflammatory cytokines both in the periphery and central nervous system (CNS).
32761702	2	40	theme	behavioral	409:418	arg1	tests					420:424	behavioral tests	409:424	behavioral tests	409:424	Animals were treated with total extracts (SCE), lignans (SCL), polysaccharides (SCPS), and essential oil (SCVO), and then subjected to behavioral tests.
32761702	7	41	theme	TLR4/NF-κB/IKKα	961:975	arg1	pathway					987:993	the TLR4/NF-κB/IKKα signaling pathway	957:993	the TLR4/NF-κB/IKKα signaling pathway	957:993	The RT-PCR results demonstrated that SCE and SCL could greatly inhibit the TLR4/NF-κB/IKKα signaling pathway.
32761702	1	42	theme	LPS-induced	256:266	arg1	mice					268:271	LPS-induced mice	256:271	LPS-induced mice	256:271	The present study aimed to examine the antidepressant actions of the composition fractions of Schisandra chinensis using LPS-induced mice.
32761702	0	43	theme	microbiota-gut-brain	73:92	arg1	axis					94:97	microbiota-gut-brain axis	73:97	microbiota-gut-brain axis	73:97	Schisandra chinensis ameliorates depressive-like behaviors by regulating microbiota-gut-brain axis via its anti-inflammation activity.
32761702	7	44	theme	signaling	977:985	arg1	pathway					987:993	the TLR4/NF-κB/IKKα signaling pathway	957:993	the TLR4/NF-κB/IKKα signaling pathway	957:993	The RT-PCR results demonstrated that SCE and SCL could greatly inhibit the TLR4/NF-κB/IKKα signaling pathway.
32761702	8	45	theme	Lactobacillus	1264:1276	arg1	members					1284:1290	Lactobacillus genus members	1264:1290	Lactobacillus genus members in LPS-induced mice	1264:1310	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	8	46	theme	relative	1179:1186	arg1	proportions					1188:1198	the relative proportions	1175:1198	the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice	1175:1310	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	8	47	theme	acid	1058:1061	arg1	concentrations					1013:1026	the concentrations	1009:1026	the concentrations of Butyric acid and Propionic acid	1009:1061	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	3	48	theme	antidepressant	530:543	arg1	activity					545:552	antidepressant activity	530:552	antidepressant activity	530:552	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	6	49	theme	colon	879:883	arg1	examination					860:870	the histopathological examination	838:870	the histopathological examination of the colon	838:883	This was confirmed by the histopathological examination of the colon.
32761702	8	50	theme	members	1284:1290	arg1	proportions					1188:1198	the relative proportions	1175:1198	the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice	1175:1310	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	2	51	theme	total	300:304	arg1	SCE					316:318	SCE	316:318	SCE	316:318	Animals were treated with total extracts (SCE), lignans (SCL), polysaccharides (SCPS), and essential oil (SCVO), and then subjected to behavioral tests.
32761702	2	51	theme	total	300:304	arg1	extracts					306:313	total extracts	300:313	total extracts (SCE)	300:319	Animals were treated with total extracts (SCE), lignans (SCL), polysaccharides (SCPS), and essential oil (SCVO), and then subjected to behavioral tests.
32761702	8	52	theme	genus	1278:1282	arg1	members					1284:1290	Lactobacillus genus members	1264:1290	Lactobacillus genus members in LPS-induced mice	1264:1310	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	8	53	theme	proportions	1188:1198	arg1	alterations					1160:1170	alterations	1160:1170	alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice	1160:1310	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	8	53	theme	proportions	1188:1198	arg1	microbiota					1145:1154	intestinal microbiota	1134:1154	intestinal microbiota	1134:1154	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	4	54	theme	Total	555:559	arg1	extracts					561:568	Total extracts	555:568	Total extracts	555:568	Total extracts and lignans significantly decreased the duration of immobility in FST and TST.
32761702	3	55	theme	forced	431:436	arg1	models					520:525	predictive animal models	502:525	predictive animal models of antidepressant activity	502:552	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	55	theme	forced	431:436	arg1	test					478:481	tail suspension test	462:481	tail suspension test (TST)	462:487	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	55	theme	forced	431:436	arg1	FST					453:455	FST	453:455	FST	453:455	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	3	55	theme	forced	431:436	arg1	test					447:450	The forced swimming test	427:450	The forced swimming test (FST)	427:456	The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity.
32761702	5	56	theme	central	786:792	arg1	system					802:807	central nervous system	786:807	central nervous system (CNS)	786:813	We found that treatment with SCE and SCL could significantly decrease the levels of pro-inflammatory cytokines both in the periphery and central nervous system (CNS).
32761702	5	56	theme	central	786:792	arg1	CNS					810:812	CNS	810:812	CNS	810:812	We found that treatment with SCE and SCL could significantly decrease the levels of pro-inflammatory cytokines both in the periphery and central nervous system (CNS).
32761702	2	57	theme	essential	365:373	arg1	SCVO					380:383	SCVO	380:383	SCVO	380:383	Animals were treated with total extracts (SCE), lignans (SCL), polysaccharides (SCPS), and essential oil (SCVO), and then subjected to behavioral tests.
32761702	2	57	theme	essential	365:373	arg1	oil					375:377	essential oil	365:377	essential oil (SCVO)	365:384	Animals were treated with total extracts (SCE), lignans (SCL), polysaccharides (SCPS), and essential oil (SCVO), and then subjected to behavioral tests.
32761702	9	58	theme	depressive	1427:1436	arg1	disorders					1438:1446	depressive disorders	1427:1446	depressive disorders	1427:1446	All results suggested that lignans is the effective fraction of S.chinensis to ameliorating depressive disorders, which its anti-inflammation activity possibly involved in the bidirectional connection between gut microbiota and brain.
32761702	8	59	theme	phylum	1232:1237	arg1	members					1239:1245	Bacteroidetes and Firmicutes phylum members	1203:1245	Bacteroidetes and Firmicutes phylum members	1203:1245	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	5	60	theme	cytokines	750:758	arg1	system					802:807	central nervous system	786:807	central nervous system (CNS)	786:813	We found that treatment with SCE and SCL could significantly decrease the levels of pro-inflammatory cytokines both in the periphery and central nervous system (CNS).
32761702	5	60	theme	cytokines	750:758	arg1	CNS					810:812	CNS	810:812	CNS	810:812	We found that treatment with SCE and SCL could significantly decrease the levels of pro-inflammatory cytokines both in the periphery and central nervous system (CNS).
32761702	5	60	theme	cytokines	750:758	arg1	levels					723:728	the levels	719:728	the levels of pro-inflammatory cytokines both in the periphery	719:780	We found that treatment with SCE and SCL could significantly decrease the levels of pro-inflammatory cytokines both in the periphery and central nervous system (CNS).
32761702	8	61	from	members	1239:1245	arg1	mice					1307:1310	LPS-induced mice	1295:1310	LPS-induced mice	1295:1310	In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS-induced mice were restored as well.
32761702	1	62	theme	antidepressant	174:187	arg1	actions					189:195	the antidepressant actions	170:195	the antidepressant actions of the composition fractions of Schisandra chinensis	170:248	The present study aimed to examine the antidepressant actions of the composition fractions of Schisandra chinensis using LPS-induced mice.
32761702	9	63	theme	effective	1377:1385	arg1	lignans					1362:1368	lignans	1362:1368	lignans	1362:1368	All results suggested that lignans is the effective fraction of S.chinensis to ameliorating depressive disorders, which its anti-inflammation activity possibly involved in the bidirectional connection between gut microbiota and brain.
32761702	9	63	theme	effective	1377:1385	arg1	fraction					1387:1394	the effective fraction	1373:1394	the effective fraction of S.chinensis to ameliorating depressive disorders, which its anti-inflammation activity possibly involved in the bidirectional connection between gut microbiota and brain	1373:1567	All results suggested that lignans is the effective fraction of S.chinensis to ameliorating depressive disorders, which its anti-inflammation activity possibly involved in the bidirectional connection between gut microbiota and brain.
33418875	0	0	theme	Metastatic	73:82	arg1	Tumors					90:95	Primary and Metastatic Brain Tumors	61:95	Primary and Metastatic Brain Tumors	61:95	The Analysis of Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors.
33418875	5	1	theme	mass	822:825	arg1	detection					841:849	single quad mass spectrometric detection	810:849	single quad mass spectrometric detection	810:849	The glycan structures were identified by the combination of single quad mass spectrometric detection and exoglycosidase digestions.
33418875	2	2	theme	inflammatory	354:365	arg1	diseases					381:388	several inflammatory and malignant diseases	346:388	several inflammatory and malignant diseases	346:388	Glycosylation is a chemical modification of proteins, reportedly altered in several inflammatory and malignant diseases, providing a potential alternative route for disease detection.
33418875	4	3	theme	PNGase-F-digested	594:610	arg1	glycans					643:649	PNGase-F-digested and procainamide-labeled serum glycans	594:649	PNGase-F-digested and procainamide-labeled serum glycans	594:649	PNGase-F-digested and procainamide-labeled serum glycans were purified by magnetic nanoparticles, followed by quantitative liquid chromatographic analysis.
33418875	5	4	theme	spectrometric	827:839	arg1	detection					841:849	single quad mass spectrometric detection	810:849	single quad mass spectrometric detection	810:849	The glycan structures were identified by the combination of single quad mass spectrometric detection and exoglycosidase digestions.
33418875	0	5	from	Analysis	4:11	arg1	Patients					47:54	Patients	47:54	Patients with Primary and Metastatic Brain Tumors	47:95	The Analysis of Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors.
33418875	2	6	theme	several	346:352	arg1	diseases					381:388	several inflammatory and malignant diseases	346:388	several inflammatory and malignant diseases	346:388	Glycosylation is a chemical modification of proteins, reportedly altered in several inflammatory and malignant diseases, providing a potential alternative route for disease detection.
33418875	7	7	theme	sialylated	1067:1076	arg1	structures					1125:1134	biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures	1046:1134	biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures	1046:1134	Altered distribution of biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures were found to be the most significant changes.
33418875	3	8	theme	N-glycosylation	514:528	arg1	analysis					496:503	the quantitative analysis	479:503	the quantitative analysis of serum N-glycosylation of patients diagnosed with primary and metastatic brain tumors	479:591	In this paper, we report the quantitative analysis of serum N-glycosylation of patients diagnosed with primary and metastatic brain tumors.
33418875	2	9	dep	altered	335:341	arg1	modification					298:309	a chemical modification	287:309	a chemical modification of proteins	287:321	Glycosylation is a chemical modification of proteins, reportedly altered in several inflammatory and malignant diseases, providing a potential alternative route for disease detection.
33418875	1	10	theme	disease	249:255	arg1	recognition					257:267	disease recognition	249:267	disease recognition	249:267	The identification of patients with different brain tumors is solely built on imaging diagnostics, indicating the need for novel methods to facilitate disease recognition.
33418875	0	11	theme	Brain	84:88	arg1	Tumors					90:95	Primary and Metastatic Brain Tumors	61:95	Primary and Metastatic Brain Tumors	61:95	The Analysis of Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors.
33418875	0	12	from	Patients	47:54	arg1	Analysis					4:11	The Analysis	0:11	The Analysis of Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors	0:95	The Analysis of Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors.
33418875	3	13	theme	primary	557:563	arg1	tumors					586:591	primary and metastatic brain tumors	557:591	primary and metastatic brain tumors	557:591	In this paper, we report the quantitative analysis of serum N-glycosylation of patients diagnosed with primary and metastatic brain tumors.
33418875	6	14	theme	Linear	882:887	arg1	analysis					902:909	Linear discriminant analysis	882:909	Linear discriminant analysis	882:909	Linear discriminant analysis provided a clear separation of different disease groups and healthy controls based on their N-glycome pattern.
33418875	3	15	gly	N-glycosylation	514:528	arg1	patients					533:540	patients	533:540	patients diagnosed with primary and metastatic brain tumors	533:591	In this paper, we report the quantitative analysis of serum N-glycosylation of patients diagnosed with primary and metastatic brain tumors.
33418875	6	16	theme	controls	979:986	arg1	separation					928:937	a clear separation	920:937	a clear separation of different disease groups and healthy controls based on their N-glycome pattern	920:1019	Linear discriminant analysis provided a clear separation of different disease groups and healthy controls based on their N-glycome pattern.
33418875	1	17	with	patients	120:127	arg1	tumors					150:155	different brain tumors	134:155	different brain tumors	134:155	The identification of patients with different brain tumors is solely built on imaging diagnostics, indicating the need for novel methods to facilitate disease recognition.
33418875	7	18	theme	neutral	1058:1064	arg1	structures					1125:1134	biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures	1046:1134	biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures	1046:1134	Altered distribution of biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures were found to be the most significant changes.
33418875	3	19	theme	patients	533:540	arg1	N-glycosylation					514:528	serum N-glycosylation	508:528	serum N-glycosylation of patients diagnosed with primary and metastatic brain tumors	508:591	In this paper, we report the quantitative analysis of serum N-glycosylation of patients diagnosed with primary and metastatic brain tumors.
33418875	2	20	gly	Glycosylation	270:282	arg1	diseases					381:388	several inflammatory and malignant diseases	346:388	several inflammatory and malignant diseases	346:388	Glycosylation is a chemical modification of proteins, reportedly altered in several inflammatory and malignant diseases, providing a potential alternative route for disease detection.
33418875	0	21	from	N-Glycosylation	28:42	arg1	Patients					47:54	Patients	47:54	Patients with Primary and Metastatic Brain Tumors	47:95	The Analysis of Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors.
33418875	5	22	theme	detection	841:849	arg1	combination					795:805	the combination	791:805	the combination of single quad mass spectrometric detection and exoglycosidase digestions	791:879	The glycan structures were identified by the combination of single quad mass spectrometric detection and exoglycosidase digestions.
33418875	7	23	theme	biantennary	1046:1056	arg1	structures					1125:1134	biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures	1046:1134	biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures	1046:1134	Altered distribution of biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures were found to be the most significant changes.
33418875	5	24	theme	single	810:815	arg1	detection					841:849	single quad mass spectrometric detection	810:849	single quad mass spectrometric detection	810:849	The glycan structures were identified by the combination of single quad mass spectrometric detection and exoglycosidase digestions.
33418875	2	25	theme	proteins	314:321	arg1	modification					298:309	a chemical modification	287:309	a chemical modification of proteins	287:321	Glycosylation is a chemical modification of proteins, reportedly altered in several inflammatory and malignant diseases, providing a potential alternative route for disease detection.
33418875	4	26	theme	liquid	717:722	arg1	analysis					740:747	quantitative liquid chromatographic analysis	704:747	quantitative liquid chromatographic analysis	704:747	PNGase-F-digested and procainamide-labeled serum glycans were purified by magnetic nanoparticles, followed by quantitative liquid chromatographic analysis.
33418875	7	27	gly	sialylated-fucosylated	1102:1123	arg1	structures					1125:1134	biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures	1046:1134	biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures	1046:1134	Altered distribution of biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures were found to be the most significant changes.
33418875	3	28	theme	metastatic	569:578	arg1	tumors					586:591	primary and metastatic brain tumors	557:591	primary and metastatic brain tumors	557:591	In this paper, we report the quantitative analysis of serum N-glycosylation of patients diagnosed with primary and metastatic brain tumors.
33418875	8	29	theme	malignancy	1274:1283	arg1	detection					1261:1269	the detection	1257:1269	the detection of malignancy	1257:1283	Our results demonstrate that serum glycosylation monitoring could improve the detection of malignancy.
33418875	1	30	theme	imaging	176:182	arg1	diagnostics					184:194	imaging diagnostics	176:194	imaging diagnostics	176:194	The identification of patients with different brain tumors is solely built on imaging diagnostics, indicating the need for novel methods to facilitate disease recognition.
33418875	0	31	theme	Human	16:20	arg1	N-Glycosylation					28:42	Human Serum N-Glycosylation	16:42	Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors	16:95	The Analysis of Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors.
33418875	5	32	theme	glycan	754:759	arg1	structures					761:770	The glycan structures	750:770	The glycan structures	750:770	The glycan structures were identified by the combination of single quad mass spectrometric detection and exoglycosidase digestions.
33418875	7	33	theme	Altered	1022:1028	arg1	distribution					1030:1041	Altered distribution	1022:1041	Altered distribution of biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures	1022:1134	Altered distribution of biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures were found to be the most significant changes.
33418875	7	33	theme	Altered	1022:1028	arg1	changes					1174:1180	the most significant changes	1153:1180	the most significant changes	1153:1180	Altered distribution of biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures were found to be the most significant changes.
33418875	4	34	theme	magnetic	668:675	arg1	nanoparticles					677:689	magnetic nanoparticles	668:689	magnetic nanoparticles	668:689	PNGase-F-digested and procainamide-labeled serum glycans were purified by magnetic nanoparticles, followed by quantitative liquid chromatographic analysis.
33418875	2	35	theme	disease	435:441	arg1	detection					443:451	disease detection	435:451	disease detection	435:451	Glycosylation is a chemical modification of proteins, reportedly altered in several inflammatory and malignant diseases, providing a potential alternative route for disease detection.
33418875	7	36	theme	sialylated-fucosylated	1102:1123	arg1	structures					1125:1134	biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures	1046:1134	biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures	1046:1134	Altered distribution of biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures were found to be the most significant changes.
33418875	6	37	theme	groups	960:965	arg1	separation					928:937	a clear separation	920:937	a clear separation of different disease groups and healthy controls based on their N-glycome pattern	920:1019	Linear discriminant analysis provided a clear separation of different disease groups and healthy controls based on their N-glycome pattern.
33418875	2	38	theme	chemical	289:296	arg1	modification					298:309	a chemical modification	287:309	a chemical modification of proteins	287:321	Glycosylation is a chemical modification of proteins, reportedly altered in several inflammatory and malignant diseases, providing a potential alternative route for disease detection.
33418875	6	39	theme	N-glycome	1003:1011	arg1	pattern					1013:1019	their N-glycome pattern	997:1019	their N-glycome pattern	997:1019	Linear discriminant analysis provided a clear separation of different disease groups and healthy controls based on their N-glycome pattern.
33418875	2	40	theme	malignant	371:379	arg1	diseases					381:388	several inflammatory and malignant diseases	346:388	several inflammatory and malignant diseases	346:388	Glycosylation is a chemical modification of proteins, reportedly altered in several inflammatory and malignant diseases, providing a potential alternative route for disease detection.
33418875	0	41	theme	N-Glycosylation	28:42	arg1	Analysis					4:11	The Analysis	0:11	The Analysis of Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors	0:95	The Analysis of Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors.
33418875	7	42	theme	significant	1162:1172	arg1	distribution					1030:1041	Altered distribution	1022:1041	Altered distribution of biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures	1022:1134	Altered distribution of biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures were found to be the most significant changes.
33418875	7	42	theme	significant	1162:1172	arg1	changes					1174:1180	the most significant changes	1153:1180	the most significant changes	1153:1180	Altered distribution of biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures were found to be the most significant changes.
33418875	6	43	theme	disease	952:958	arg1	groups					960:965	different disease groups	942:965	different disease groups	942:965	Linear discriminant analysis provided a clear separation of different disease groups and healthy controls based on their N-glycome pattern.
33418875	4	44	theme	quantitative	704:715	arg1	analysis					740:747	quantitative liquid chromatographic analysis	704:747	quantitative liquid chromatographic analysis	704:747	PNGase-F-digested and procainamide-labeled serum glycans were purified by magnetic nanoparticles, followed by quantitative liquid chromatographic analysis.
33418875	2	45	theme	alternative	413:423	arg1	route					425:429	a potential alternative route	401:429	a potential alternative route for disease detection	401:451	Glycosylation is a chemical modification of proteins, reportedly altered in several inflammatory and malignant diseases, providing a potential alternative route for disease detection.
33418875	0	46	theme	Serum	22:26	arg1	N-Glycosylation					28:42	Human Serum N-Glycosylation	16:42	Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors	16:95	The Analysis of Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors.
33418875	6	47	theme	different	942:950	arg1	groups					960:965	different disease groups	942:965	different disease groups	942:965	Linear discriminant analysis provided a clear separation of different disease groups and healthy controls based on their N-glycome pattern.
33418875	2	48	theme	potential	403:411	arg1	route					425:429	a potential alternative route	401:429	a potential alternative route for disease detection	401:451	Glycosylation is a chemical modification of proteins, reportedly altered in several inflammatory and malignant diseases, providing a potential alternative route for disease detection.
33418875	5	49	theme	exoglycosidase	855:868	arg1	digestions					870:879	exoglycosidase digestions	855:879	exoglycosidase digestions	855:879	The glycan structures were identified by the combination of single quad mass spectrometric detection and exoglycosidase digestions.
33418875	5	50	theme	quad	817:820	arg1	detection					841:849	single quad mass spectrometric detection	810:849	single quad mass spectrometric detection	810:849	The glycan structures were identified by the combination of single quad mass spectrometric detection and exoglycosidase digestions.
33418875	6	51	theme	healthy	971:977	arg1	controls					979:986	healthy controls	971:986	healthy controls	971:986	Linear discriminant analysis provided a clear separation of different disease groups and healthy controls based on their N-glycome pattern.
33418875	3	52	theme	quantitative	483:494	arg1	analysis					496:503	the quantitative analysis	479:503	the quantitative analysis of serum N-glycosylation of patients diagnosed with primary and metastatic brain tumors	479:591	In this paper, we report the quantitative analysis of serum N-glycosylation of patients diagnosed with primary and metastatic brain tumors.
33418875	5	53	theme	digestions	870:879	arg1	combination					795:805	the combination	791:805	the combination of single quad mass spectrometric detection and exoglycosidase digestions	791:879	The glycan structures were identified by the combination of single quad mass spectrometric detection and exoglycosidase digestions.
33418875	8	54	theme	glycosylation	1218:1230	arg1	monitoring					1232:1241	serum glycosylation monitoring	1212:1241	serum glycosylation monitoring	1212:1241	Our results demonstrate that serum glycosylation monitoring could improve the detection of malignancy.
33418875	4	55	dep	purified	656:663	arg1	followed					692:699	followed	692:699	followed by quantitative liquid chromatographic analysis	692:747	PNGase-F-digested and procainamide-labeled serum glycans were purified by magnetic nanoparticles, followed by quantitative liquid chromatographic analysis.
33418875	3	56	theme	serum	508:512	arg1	N-glycosylation					514:528	serum N-glycosylation	508:528	serum N-glycosylation of patients diagnosed with primary and metastatic brain tumors	508:591	In this paper, we report the quantitative analysis of serum N-glycosylation of patients diagnosed with primary and metastatic brain tumors.
33418875	3	57	theme	brain	580:584	arg1	tumors					586:591	primary and metastatic brain tumors	557:591	primary and metastatic brain tumors	557:591	In this paper, we report the quantitative analysis of serum N-glycosylation of patients diagnosed with primary and metastatic brain tumors.
33418875	8	58	theme	serum	1212:1216	arg1	monitoring					1232:1241	serum glycosylation monitoring	1212:1241	serum glycosylation monitoring	1212:1241	Our results demonstrate that serum glycosylation monitoring could improve the detection of malignancy.
33418875	6	59	theme	clear	922:926	arg1	separation					928:937	a clear separation	920:937	a clear separation of different disease groups and healthy controls based on their N-glycome pattern	920:1019	Linear discriminant analysis provided a clear separation of different disease groups and healthy controls based on their N-glycome pattern.
33418875	6	60	theme	discriminant	889:900	arg1	analysis					902:909	Linear discriminant analysis	882:909	Linear discriminant analysis	882:909	Linear discriminant analysis provided a clear separation of different disease groups and healthy controls based on their N-glycome pattern.
33418875	1	61	theme	patients	120:127	arg1	identification					102:115	The identification	98:115	The identification of patients with different brain tumors	98:155	The identification of patients with different brain tumors is solely built on imaging diagnostics, indicating the need for novel methods to facilitate disease recognition.
33418875	7	62	gly	sialylated	1067:1076	arg1	structures					1125:1134	biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures	1046:1134	biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures	1046:1134	Altered distribution of biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures were found to be the most significant changes.
33418875	7	63	theme	structures	1125:1134	arg1	distribution					1030:1041	Altered distribution	1022:1041	Altered distribution of biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures	1022:1134	Altered distribution of biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures were found to be the most significant changes.
33418875	7	63	theme	structures	1125:1134	arg1	changes					1174:1180	the most significant changes	1153:1180	the most significant changes	1153:1180	Altered distribution of biantennary neutral, sialylated but nonfucosylated, and sialylated-fucosylated structures were found to be the most significant changes.
33418875	0	64	with	Patients	47:54	arg1	Tumors					90:95	Primary and Metastatic Brain Tumors	61:95	Primary and Metastatic Brain Tumors	61:95	The Analysis of Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors.
33418875	4	65	theme	chromatographic	724:738	arg1	analysis					740:747	quantitative liquid chromatographic analysis	704:747	quantitative liquid chromatographic analysis	704:747	PNGase-F-digested and procainamide-labeled serum glycans were purified by magnetic nanoparticles, followed by quantitative liquid chromatographic analysis.
33418875	4	66	theme	serum	637:641	arg1	glycans					643:649	PNGase-F-digested and procainamide-labeled serum glycans	594:649	PNGase-F-digested and procainamide-labeled serum glycans	594:649	PNGase-F-digested and procainamide-labeled serum glycans were purified by magnetic nanoparticles, followed by quantitative liquid chromatographic analysis.
33418875	0	67	theme	Primary	61:67	arg1	Tumors					90:95	Primary and Metastatic Brain Tumors	61:95	Primary and Metastatic Brain Tumors	61:95	The Analysis of Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors.
33418875	1	68	theme	different	134:142	arg1	tumors					150:155	different brain tumors	134:155	different brain tumors	134:155	The identification of patients with different brain tumors is solely built on imaging diagnostics, indicating the need for novel methods to facilitate disease recognition.
33418875	1	69	theme	novel	221:225	arg1	methods					227:233	novel methods	221:233	novel methods	221:233	The identification of patients with different brain tumors is solely built on imaging diagnostics, indicating the need for novel methods to facilitate disease recognition.
33418875	4	70	theme	procainamide-labeled	616:635	arg1	glycans					643:649	PNGase-F-digested and procainamide-labeled serum glycans	594:649	PNGase-F-digested and procainamide-labeled serum glycans	594:649	PNGase-F-digested and procainamide-labeled serum glycans were purified by magnetic nanoparticles, followed by quantitative liquid chromatographic analysis.
33418875	1	71	theme	brain	144:148	arg1	tumors					150:155	different brain tumors	134:155	different brain tumors	134:155	The identification of patients with different brain tumors is solely built on imaging diagnostics, indicating the need for novel methods to facilitate disease recognition.
32388896	5	0	theme	oligosaccharides	992:1007	arg1	analysis					964:971	Qualitative on-line liquid chromatography-mass spectrometric analysis	903:971	Qualitative on-line liquid chromatography-mass spectrometric analysis of these resistant oligosaccharides	903:1007	Qualitative on-line liquid chromatography-mass spectrometric analysis of these resistant oligosaccharides is also described in this paper.
32388896	1	1	from	ends	369:372	arg1	residues					342:349	3-O-sulfo group-containing glucosamine residues	303:349	3-O-sulfo group-containing glucosamine residues at their reducing ends	303:372	Complete heparin digestion with heparin lyase I and II results in a mixture of hexasaccharides and tetrasaccharides with 3-O-sulfo group-containing glucosamine residues at their reducing ends.
32388896	5	2	theme	on-line	915:921	arg1	analysis					964:971	Qualitative on-line liquid chromatography-mass spectrometric analysis	903:971	Qualitative on-line liquid chromatography-mass spectrometric analysis of these resistant oligosaccharides	903:1007	Qualitative on-line liquid chromatography-mass spectrometric analysis of these resistant oligosaccharides is also described in this paper.
32388896	1	3	theme	heparin	191:197	arg1	digestion					199:207	Complete heparin digestion	182:207	Complete heparin digestion with heparin lyase I and II	182:235	Complete heparin digestion with heparin lyase I and II results in a mixture of hexasaccharides and tetrasaccharides with 3-O-sulfo group-containing glucosamine residues at their reducing ends.
32388896	2	4	attach	derived	410:416	arg1	heparin					457:463	heparin	457:463	heparin	457:463	Because these tetrasaccharides are derived from antithrombin III-binding sites of heparin, we examined whether this method could be applied to estimate the anticoagulant activity of heparin.
32388896	2	4	attach	derived	410:416	arg1	sites					448:452	antithrombin III-binding sites	423:452	antithrombin III-binding sites of heparin	423:463	Because these tetrasaccharides are derived from antithrombin III-binding sites of heparin, we examined whether this method could be applied to estimate the anticoagulant activity of heparin.
32388896	2	4	attach	derived	410:416	arg2	tetrasaccharides					389:404	these tetrasaccharides	383:404	these tetrasaccharides	383:404	Because these tetrasaccharides are derived from antithrombin III-binding sites of heparin, we examined whether this method could be applied to estimate the anticoagulant activity of heparin.
32388896	1	5	with	digestion	199:207	arg1	lyase					222:226	heparin lyase I and II	214:235	lyase	222:226	Complete heparin digestion with heparin lyase I and II results in a mixture of hexasaccharides and tetrasaccharides with 3-O-sulfo group-containing glucosamine residues at their reducing ends.
32388896	1	5	with	digestion	199:207	arg1	II					234:235	II	234:235	II	234:235	Complete heparin digestion with heparin lyase I and II results in a mixture of hexasaccharides and tetrasaccharides with 3-O-sulfo group-containing glucosamine residues at their reducing ends.
32388896	4	6	theme	important	776:784	arg1	analysis					697:704	Qualitative analysis	685:704	Qualitative analysis of the studied compounds	685:729	Qualitative analysis of the studied compounds and a comparison of their composition are an important contribution to the structural analysis of low molecular weight heparins, which has not been fully conducted so far.
32388896	4	6	theme	important	776:784	arg1	contribution					786:797	an important contribution	773:797	an important contribution	773:797	Qualitative analysis of the studied compounds and a comparison of their composition are an important contribution to the structural analysis of low molecular weight heparins, which has not been fully conducted so far.
32388896	4	6	theme	important	776:784	arg1	comparison					737:746	a comparison	735:746	a comparison of their composition	735:767	Qualitative analysis of the studied compounds and a comparison of their composition are an important contribution to the structural analysis of low molecular weight heparins, which has not been fully conducted so far.
32388896	1	7	theme	tetrasaccharides	281:296	arg1	mixture					250:256	a mixture	248:256	a mixture of hexasaccharides and tetrasaccharides with 3-O-sulfo group-containing glucosamine residues at their reducing ends	248:372	Complete heparin digestion with heparin lyase I and II results in a mixture of hexasaccharides and tetrasaccharides with 3-O-sulfo group-containing glucosamine residues at their reducing ends.
32388896	3	8	theme	new	599:601	arg1	heparin					624:630	a new low molecular weight heparin	597:630	a new low molecular weight heparin sample preparation method-chemical depolymerization	597:682	Therefore, this paper presents a new low molecular weight heparin sample preparation method-chemical depolymerization.
32388896	4	9	theme	molecular	833:841	arg1	heparins					850:857	low molecular weight heparins	829:857	low molecular weight heparins	829:857	Qualitative analysis of the studied compounds and a comparison of their composition are an important contribution to the structural analysis of low molecular weight heparins, which has not been fully conducted so far.
32388896	4	10	theme	low	829:831	arg1	heparins					850:857	low molecular weight heparins	829:857	low molecular weight heparins	829:857	Qualitative analysis of the studied compounds and a comparison of their composition are an important contribution to the structural analysis of low molecular weight heparins, which has not been fully conducted so far.
32388896	4	11	theme	heparins	850:857	arg1	analysis					817:824	the structural analysis	802:824	the structural analysis of low molecular weight heparins	802:857	Qualitative analysis of the studied compounds and a comparison of their composition are an important contribution to the structural analysis of low molecular weight heparins, which has not been fully conducted so far.
32388896	3	12	theme	low	603:605	arg1	heparin					624:630	a new low molecular weight heparin	597:630	a new low molecular weight heparin sample preparation method-chemical depolymerization	597:682	Therefore, this paper presents a new low molecular weight heparin sample preparation method-chemical depolymerization.
32388896	1	13	theme	3-O-sulfo	303:311	arg1	residues					342:349	3-O-sulfo group-containing glucosamine residues	303:349	3-O-sulfo group-containing glucosamine residues at their reducing ends	303:372	Complete heparin digestion with heparin lyase I and II results in a mixture of hexasaccharides and tetrasaccharides with 3-O-sulfo group-containing glucosamine residues at their reducing ends.
32388896	0	14	theme	electrospray	113:124	arg1	spectrometry					168:179	electrospray ionization quadrupole time-of-flight-mass spectrometry	113:179	electrospray ionization quadrupole time-of-flight-mass spectrometry	113:179	Qualitative analysis of enzymatic and chemical depolymerized low molecular weight heparins by UHPLC coupled with electrospray ionization quadrupole time-of-flight-mass spectrometry.
32388896	5	15	theme	liquid	923:928	arg1	analysis					964:971	Qualitative on-line liquid chromatography-mass spectrometric analysis	903:971	Qualitative on-line liquid chromatography-mass spectrometric analysis of these resistant oligosaccharides	903:1007	Qualitative on-line liquid chromatography-mass spectrometric analysis of these resistant oligosaccharides is also described in this paper.
32388896	2	16	theme	anticoagulant	531:543	arg1	activity					545:552	the anticoagulant activity	527:552	the anticoagulant activity of heparin	527:563	Because these tetrasaccharides are derived from antithrombin III-binding sites of heparin, we examined whether this method could be applied to estimate the anticoagulant activity of heparin.
32388896	1	17	theme	heparin	214:220	arg1	lyase					222:226	heparin lyase I and II	214:235	lyase	222:226	Complete heparin digestion with heparin lyase I and II results in a mixture of hexasaccharides and tetrasaccharides with 3-O-sulfo group-containing glucosamine residues at their reducing ends.
32388896	3	18	theme	heparin	624:630	arg1	preparation					639:649	a new low molecular weight heparin sample preparation	597:649	a new low molecular weight heparin sample preparation method-chemical depolymerization	597:682	Therefore, this paper presents a new low molecular weight heparin sample preparation method-chemical depolymerization.
32388896	4	19	theme	composition	757:767	arg1	analysis					697:704	Qualitative analysis	685:704	Qualitative analysis of the studied compounds	685:729	Qualitative analysis of the studied compounds and a comparison of their composition are an important contribution to the structural analysis of low molecular weight heparins, which has not been fully conducted so far.
32388896	4	19	theme	composition	757:767	arg1	contribution					786:797	an important contribution	773:797	an important contribution	773:797	Qualitative analysis of the studied compounds and a comparison of their composition are an important contribution to the structural analysis of low molecular weight heparins, which has not been fully conducted so far.
32388896	4	19	theme	composition	757:767	arg1	comparison					737:746	a comparison	735:746	a comparison of their composition	735:767	Qualitative analysis of the studied compounds and a comparison of their composition are an important contribution to the structural analysis of low molecular weight heparins, which has not been fully conducted so far.
32388896	0	20	theme	Qualitative	0:10	arg1	analysis					12:19	Qualitative analysis	0:19	Qualitative analysis of enzymatic and chemical	0:45	Qualitative analysis of enzymatic and chemical depolymerized low molecular weight heparins by UHPLC coupled with electrospray ionization quadrupole time-of-flight-mass spectrometry.
32388896	1	21	theme	group-containing	313:328	arg1	residues					342:349	3-O-sulfo group-containing glucosamine residues	303:349	3-O-sulfo group-containing glucosamine residues at their reducing ends	303:372	Complete heparin digestion with heparin lyase I and II results in a mixture of hexasaccharides and tetrasaccharides with 3-O-sulfo group-containing glucosamine residues at their reducing ends.
32388896	4	22	theme	structural	806:815	arg1	analysis					817:824	the structural analysis	802:824	the structural analysis of low molecular weight heparins	802:857	Qualitative analysis of the studied compounds and a comparison of their composition are an important contribution to the structural analysis of low molecular weight heparins, which has not been fully conducted so far.
32388896	3	23	theme	sample	632:637	arg1	preparation					639:649	a new low molecular weight heparin sample preparation	597:649	a new low molecular weight heparin sample preparation method-chemical depolymerization	597:682	Therefore, this paper presents a new low molecular weight heparin sample preparation method-chemical depolymerization.
32388896	5	24	theme	resistant	982:990	arg1	oligosaccharides					992:1007	these resistant oligosaccharides	976:1007	these resistant oligosaccharides	976:1007	Qualitative on-line liquid chromatography-mass spectrometric analysis of these resistant oligosaccharides is also described in this paper.
32388896	0	25	theme	enzymatic	24:32	arg1	analysis					12:19	Qualitative analysis	0:19	Qualitative analysis of enzymatic and chemical	0:45	Qualitative analysis of enzymatic and chemical depolymerized low molecular weight heparins by UHPLC coupled with electrospray ionization quadrupole time-of-flight-mass spectrometry.
32388896	1	26	theme	glucosamine	330:340	arg1	residues					342:349	3-O-sulfo group-containing glucosamine residues	303:349	3-O-sulfo group-containing glucosamine residues at their reducing ends	303:372	Complete heparin digestion with heparin lyase I and II results in a mixture of hexasaccharides and tetrasaccharides with 3-O-sulfo group-containing glucosamine residues at their reducing ends.
32388896	0	27	theme	quadrupole	137:146	arg1	spectrometry					168:179	electrospray ionization quadrupole time-of-flight-mass spectrometry	113:179	electrospray ionization quadrupole time-of-flight-mass spectrometry	113:179	Qualitative analysis of enzymatic and chemical depolymerized low molecular weight heparins by UHPLC coupled with electrospray ionization quadrupole time-of-flight-mass spectrometry.
32388896	3	28	theme	molecular	607:615	arg1	heparin					624:630	a new low molecular weight heparin	597:630	a new low molecular weight heparin sample preparation method-chemical depolymerization	597:682	Therefore, this paper presents a new low molecular weight heparin sample preparation method-chemical depolymerization.
32388896	4	29	theme	compounds	721:729	arg1	analysis					697:704	Qualitative analysis	685:704	Qualitative analysis of the studied compounds	685:729	Qualitative analysis of the studied compounds and a comparison of their composition are an important contribution to the structural analysis of low molecular weight heparins, which has not been fully conducted so far.
32388896	4	29	theme	compounds	721:729	arg1	contribution					786:797	an important contribution	773:797	an important contribution	773:797	Qualitative analysis of the studied compounds and a comparison of their composition are an important contribution to the structural analysis of low molecular weight heparins, which has not been fully conducted so far.
32388896	4	29	theme	compounds	721:729	arg1	comparison					737:746	a comparison	735:746	a comparison of their composition	735:767	Qualitative analysis of the studied compounds and a comparison of their composition are an important contribution to the structural analysis of low molecular weight heparins, which has not been fully conducted so far.
32388896	5	30	theme	Qualitative	903:913	arg1	analysis					964:971	Qualitative on-line liquid chromatography-mass spectrometric analysis	903:971	Qualitative on-line liquid chromatography-mass spectrometric analysis of these resistant oligosaccharides	903:1007	Qualitative on-line liquid chromatography-mass spectrometric analysis of these resistant oligosaccharides is also described in this paper.
32388896	0	31	theme	ionization	126:135	arg1	spectrometry					168:179	electrospray ionization quadrupole time-of-flight-mass spectrometry	113:179	electrospray ionization quadrupole time-of-flight-mass spectrometry	113:179	Qualitative analysis of enzymatic and chemical depolymerized low molecular weight heparins by UHPLC coupled with electrospray ionization quadrupole time-of-flight-mass spectrometry.
32388896	3	32	theme	weight	617:622	arg1	heparin					624:630	a new low molecular weight heparin	597:630	a new low molecular weight heparin sample preparation method-chemical depolymerization	597:682	Therefore, this paper presents a new low molecular weight heparin sample preparation method-chemical depolymerization.
32388896	4	33	theme	studied	713:719	arg1	compounds					721:729	the studied compounds	709:729	the studied compounds	709:729	Qualitative analysis of the studied compounds and a comparison of their composition are an important contribution to the structural analysis of low molecular weight heparins, which has not been fully conducted so far.
32388896	0	34	theme	chemical	38:45	arg1	analysis					12:19	Qualitative analysis	0:19	Qualitative analysis of enzymatic and chemical	0:45	Qualitative analysis of enzymatic and chemical depolymerized low molecular weight heparins by UHPLC coupled with electrospray ionization quadrupole time-of-flight-mass spectrometry.
32388896	5	35	theme	chromatography-mass	930:948	arg1	analysis					964:971	Qualitative on-line liquid chromatography-mass spectrometric analysis	903:971	Qualitative on-line liquid chromatography-mass spectrometric analysis of these resistant oligosaccharides	903:1007	Qualitative on-line liquid chromatography-mass spectrometric analysis of these resistant oligosaccharides is also described in this paper.
32388896	0	36	theme	time-of-flight-mass	148:166	arg1	spectrometry					168:179	electrospray ionization quadrupole time-of-flight-mass spectrometry	113:179	electrospray ionization quadrupole time-of-flight-mass spectrometry	113:179	Qualitative analysis of enzymatic and chemical depolymerized low molecular weight heparins by UHPLC coupled with electrospray ionization quadrupole time-of-flight-mass spectrometry.
32388896	5	37	theme	spectrometric	950:962	arg1	analysis					964:971	Qualitative on-line liquid chromatography-mass spectrometric analysis	903:971	Qualitative on-line liquid chromatography-mass spectrometric analysis of these resistant oligosaccharides	903:1007	Qualitative on-line liquid chromatography-mass spectrometric analysis of these resistant oligosaccharides is also described in this paper.
32388896	0	38	theme	low	61:63	arg1	heparins					82:89	low molecular weight heparins	61:89	low molecular weight heparins	61:89	Qualitative analysis of enzymatic and chemical depolymerized low molecular weight heparins by UHPLC coupled with electrospray ionization quadrupole time-of-flight-mass spectrometry.
32388896	1	39	theme	reducing	360:367	arg1	ends					369:372	their reducing ends	354:372	their reducing ends	354:372	Complete heparin digestion with heparin lyase I and II results in a mixture of hexasaccharides and tetrasaccharides with 3-O-sulfo group-containing glucosamine residues at their reducing ends.
32388896	2	40	theme	heparin	557:563	arg1	activity					545:552	the anticoagulant activity	527:552	the anticoagulant activity of heparin	527:563	Because these tetrasaccharides are derived from antithrombin III-binding sites of heparin, we examined whether this method could be applied to estimate the anticoagulant activity of heparin.
32388896	3	41	theme	preparation	639:649	arg1	depolymerization					667:682	a new low molecular weight heparin sample preparation method-chemical depolymerization	597:682	a new low molecular weight heparin sample preparation method-chemical depolymerization	597:682	Therefore, this paper presents a new low molecular weight heparin sample preparation method-chemical depolymerization.
32388896	3	42	theme	method-chemical	651:665	arg1	depolymerization					667:682	a new low molecular weight heparin sample preparation method-chemical depolymerization	597:682	a new low molecular weight heparin sample preparation method-chemical depolymerization	597:682	Therefore, this paper presents a new low molecular weight heparin sample preparation method-chemical depolymerization.
32388896	1	43	with	tetrasaccharides	281:296	arg1	residues					342:349	3-O-sulfo group-containing glucosamine residues	303:349	3-O-sulfo group-containing glucosamine residues at their reducing ends	303:372	Complete heparin digestion with heparin lyase I and II results in a mixture of hexasaccharides and tetrasaccharides with 3-O-sulfo group-containing glucosamine residues at their reducing ends.
32388896	4	44	theme	weight	843:848	arg1	heparins					850:857	low molecular weight heparins	829:857	low molecular weight heparins	829:857	Qualitative analysis of the studied compounds and a comparison of their composition are an important contribution to the structural analysis of low molecular weight heparins, which has not been fully conducted so far.
32388896	2	45	theme	antithrombin	423:434	arg1	heparin					457:463	heparin	457:463	heparin	457:463	Because these tetrasaccharides are derived from antithrombin III-binding sites of heparin, we examined whether this method could be applied to estimate the anticoagulant activity of heparin.
32388896	2	45	theme	antithrombin	423:434	arg1	sites					448:452	antithrombin III-binding sites	423:452	antithrombin III-binding sites of heparin	423:463	Because these tetrasaccharides are derived from antithrombin III-binding sites of heparin, we examined whether this method could be applied to estimate the anticoagulant activity of heparin.
32388896	4	46	theme	Qualitative	685:695	arg1	analysis					697:704	Qualitative analysis	685:704	Qualitative analysis of the studied compounds	685:729	Qualitative analysis of the studied compounds and a comparison of their composition are an important contribution to the structural analysis of low molecular weight heparins, which has not been fully conducted so far.
32388896	4	46	theme	Qualitative	685:695	arg1	contribution					786:797	an important contribution	773:797	an important contribution	773:797	Qualitative analysis of the studied compounds and a comparison of their composition are an important contribution to the structural analysis of low molecular weight heparins, which has not been fully conducted so far.
32388896	4	46	theme	Qualitative	685:695	arg1	comparison					737:746	a comparison	735:746	a comparison of their composition	735:767	Qualitative analysis of the studied compounds and a comparison of their composition are an important contribution to the structural analysis of low molecular weight heparins, which has not been fully conducted so far.
32388896	0	47	theme	weight	75:80	arg1	heparins					82:89	low molecular weight heparins	61:89	low molecular weight heparins	61:89	Qualitative analysis of enzymatic and chemical depolymerized low molecular weight heparins by UHPLC coupled with electrospray ionization quadrupole time-of-flight-mass spectrometry.
32388896	1	48	with	hexasaccharides	261:275	arg1	residues					342:349	3-O-sulfo group-containing glucosamine residues	303:349	3-O-sulfo group-containing glucosamine residues at their reducing ends	303:372	Complete heparin digestion with heparin lyase I and II results in a mixture of hexasaccharides and tetrasaccharides with 3-O-sulfo group-containing glucosamine residues at their reducing ends.
32388896	2	49	theme	III-binding	436:446	arg1	heparin					457:463	heparin	457:463	heparin	457:463	Because these tetrasaccharides are derived from antithrombin III-binding sites of heparin, we examined whether this method could be applied to estimate the anticoagulant activity of heparin.
32388896	2	49	theme	III-binding	436:446	arg1	sites					448:452	antithrombin III-binding sites	423:452	antithrombin III-binding sites of heparin	423:463	Because these tetrasaccharides are derived from antithrombin III-binding sites of heparin, we examined whether this method could be applied to estimate the anticoagulant activity of heparin.
32388896	2	50	theme	heparin	457:463	arg1	heparin					457:463	heparin	457:463	heparin	457:463	Because these tetrasaccharides are derived from antithrombin III-binding sites of heparin, we examined whether this method could be applied to estimate the anticoagulant activity of heparin.
32388896	2	50	theme	heparin	457:463	arg1	sites					448:452	antithrombin III-binding sites	423:452	antithrombin III-binding sites of heparin	423:463	Because these tetrasaccharides are derived from antithrombin III-binding sites of heparin, we examined whether this method could be applied to estimate the anticoagulant activity of heparin.
32388896	0	51	theme	molecular	65:73	arg1	heparins					82:89	low molecular weight heparins	61:89	low molecular weight heparins	61:89	Qualitative analysis of enzymatic and chemical depolymerized low molecular weight heparins by UHPLC coupled with electrospray ionization quadrupole time-of-flight-mass spectrometry.
32388896	1	52	theme	Complete	182:189	arg1	digestion					199:207	Complete heparin digestion	182:207	Complete heparin digestion with heparin lyase I and II	182:235	Complete heparin digestion with heparin lyase I and II results in a mixture of hexasaccharides and tetrasaccharides with 3-O-sulfo group-containing glucosamine residues at their reducing ends.
32388896	1	53	theme	hexasaccharides	261:275	arg1	mixture					250:256	a mixture	248:256	a mixture of hexasaccharides and tetrasaccharides with 3-O-sulfo group-containing glucosamine residues at their reducing ends	248:372	Complete heparin digestion with heparin lyase I and II results in a mixture of hexasaccharides and tetrasaccharides with 3-O-sulfo group-containing glucosamine residues at their reducing ends.
33342609	5	0	theme	proteins	652:659	arg1	proteins					652:659	proteins	652:659	proteins	652:659	Fractions also contained 10-36% of proteins.
33342609	5	0	theme	proteins	652:659	arg1	%					647:647	10-36%	642:647	10-36% of proteins	642:659	Fractions also contained 10-36% of proteins.
33342609	2	1	theme	temperature	341:351	arg1	water					353:357	room temperature water	336:357	room temperature water	336:357	To envisage potential applications, cell wall sequential extraction performed on dry plant yielded 1.1, 2.4, 0.3 and 0.9% of pectin fractions respectively extracted by room temperature water, 90 °C-water, CDTA and Na2CO3.
33342609	0	2	theme	functional	68:77	arg1	properties					79:88	functional properties	68:88	functional properties of its biopolymers	68:107	Perennial halophyte Salicornia neei Lag.: Cell wall composition and functional properties of its biopolymers.
33342609	2	3	theme	room	336:339	arg1	water					353:357	room temperature water	336:357	room temperature water	336:357	To envisage potential applications, cell wall sequential extraction performed on dry plant yielded 1.1, 2.4, 0.3 and 0.9% of pectin fractions respectively extracted by room temperature water, 90 °C-water, CDTA and Na2CO3.
33342609	3	4	with	ratios	475:480	arg1	degree					439:444	low degree	435:444	low degree of methylation	435:459	They contained 21-33% uronic acids (UA) with low degree of methylation and 0.5-1.2 M ratios of neutral sugars to UA.
33342609	4	5	theme	arabinan	547:554	arg1	side-chains					556:566	long arabinan side-chains	542:566	long arabinan side-chains	542:566	High arabinose level suggests that long arabinan side-chains maintain cell wall flexibility in water deficit.
33342609	8	6	theme	final	916:920	arg1	residue					922:928	the final residue	912:928	the final residue	912:928	Cellulose (28%) and lignin (45.1%) were the main biopolymers in the final residue, which showed low water swelling capacity (3.6 mL/g) due to lignin, increasing when arabinoxylans were also present.
33342609	3	7	contain	contained	395:403	arg2	acids					419:423	21-33% uronic acids	405:423	21-33% uronic acids (UA) with low degree of methylation	405:459	They contained 21-33% uronic acids (UA) with low degree of methylation and 0.5-1.2 M ratios of neutral sugars to UA.
33342609	3	7	contain	contained	395:403	arg2	UA					426:427	UA	426:427	UA	426:427	They contained 21-33% uronic acids (UA) with low degree of methylation and 0.5-1.2 M ratios of neutral sugars to UA.
33342609	3	7	contain	contained	395:403	arg2	ratios					475:480	0.5-1.2 M ratios	465:480	0.5-1.2 M ratios of neutral sugars to UA	465:504	They contained 21-33% uronic acids (UA) with low degree of methylation and 0.5-1.2 M ratios of neutral sugars to UA.
33342609	3	7	contain	contained	395:403	arg1	They					390:393	They	390:393	They	390:393	They contained 21-33% uronic acids (UA) with low degree of methylation and 0.5-1.2 M ratios of neutral sugars to UA.
33342609	10	8	theme	useful	1162:1167	arg1	biopolymers					1121:1131	biopolymers	1121:1131	biopolymers	1121:1131	Salicornia is a source of biopolymers and antioxidants potentially useful for food applications.
33342609	4	9	theme	long	542:545	arg1	side-chains					556:566	long arabinan side-chains	542:566	long arabinan side-chains	542:566	High arabinose level suggests that long arabinan side-chains maintain cell wall flexibility in water deficit.
33342609	3	10	theme	neutral	485:491	arg1	sugars					493:498	neutral sugars	485:498	neutral sugars	485:498	They contained 21-33% uronic acids (UA) with low degree of methylation and 0.5-1.2 M ratios of neutral sugars to UA.
33342609	6	11	theme	KOH-soluble	666:676	arg1	%					692:692	4.3%	689:692	4.3%	689:692	The KOH-soluble fractions (4.3%) were mainly arabinoxylans.
33342609	6	11	theme	KOH-soluble	666:676	arg1	arabinoxylans					707:719	arabinoxylans	707:719	arabinoxylans	707:719	The KOH-soluble fractions (4.3%) were mainly arabinoxylans.
33342609	6	11	theme	KOH-soluble	666:676	arg1	fractions					678:686	The KOH-soluble fractions	662:686	The KOH-soluble fractions (4.3%)	662:693	The KOH-soluble fractions (4.3%) were mainly arabinoxylans.
33342609	3	12	theme	uronic	412:417	arg1	UA					426:427	UA	426:427	UA	426:427	They contained 21-33% uronic acids (UA) with low degree of methylation and 0.5-1.2 M ratios of neutral sugars to UA.
33342609	3	12	theme	uronic	412:417	arg1	acids					419:423	21-33% uronic acids	405:423	21-33% uronic acids (UA) with low degree of methylation	405:459	They contained 21-33% uronic acids (UA) with low degree of methylation and 0.5-1.2 M ratios of neutral sugars to UA.
33342609	0	13	theme	biopolymers	97:107	arg1	composition					52:62	Cell wall composition	42:62	Cell wall composition	42:62	Perennial halophyte Salicornia neei Lag.: Cell wall composition and functional properties of its biopolymers.
33342609	0	13	theme	biopolymers	97:107	arg1	properties					79:88	functional properties	68:88	functional properties of its biopolymers	68:107	Perennial halophyte Salicornia neei Lag.: Cell wall composition and functional properties of its biopolymers.
33342609	3	14	theme	sugars	493:498	arg1	UA					426:427	UA	426:427	UA	426:427	They contained 21-33% uronic acids (UA) with low degree of methylation and 0.5-1.2 M ratios of neutral sugars to UA.
33342609	3	14	theme	sugars	493:498	arg1	acids					419:423	21-33% uronic acids	405:423	21-33% uronic acids (UA) with low degree of methylation	405:459	They contained 21-33% uronic acids (UA) with low degree of methylation and 0.5-1.2 M ratios of neutral sugars to UA.
33342609	3	14	theme	sugars	493:498	arg1	ratios					475:480	0.5-1.2 M ratios	465:480	0.5-1.2 M ratios of neutral sugars to UA	465:504	They contained 21-33% uronic acids (UA) with low degree of methylation and 0.5-1.2 M ratios of neutral sugars to UA.
33342609	7	15	theme	-type	772:776	arg1	networks					778:785	"weak gel"-type networks	762:785	"weak gel"-type networks	762:785	At 2.0% w/v, pectin fractions developed "weak gel"-type networks with Ca2+, while arabinoxylans generated "dilute solutions".
33342609	4	16	theme	arabinose	512:520	arg1	level					522:526	High arabinose level	507:526	High arabinose level	507:526	High arabinose level suggests that long arabinan side-chains maintain cell wall flexibility in water deficit.
33342609	8	17	theme	low	944:946	arg1	capacity					963:970	low water swelling capacity	944:970	low water swelling capacity (3.6 mL/g)	944:981	Cellulose (28%) and lignin (45.1%) were the main biopolymers in the final residue, which showed low water swelling capacity (3.6 mL/g) due to lignin, increasing when arabinoxylans were also present.
33342609	8	17	theme	low	944:946	arg1	3.6 mL/g					973:980	3.6 mL/g	973:980	3.6 mL/g	973:980	Cellulose (28%) and lignin (45.1%) were the main biopolymers in the final residue, which showed low water swelling capacity (3.6 mL/g) due to lignin, increasing when arabinoxylans were also present.
33342609	2	18	theme	pectin	293:298	arg1	fractions					300:308	pectin fractions	293:308	pectin fractions respectively extracted by room temperature water, 90 °C-water, CDTA and Na2CO3	293:387	To envisage potential applications, cell wall sequential extraction performed on dry plant yielded 1.1, 2.4, 0.3 and 0.9% of pectin fractions respectively extracted by room temperature water, 90 °C-water, CDTA and Na2CO3.
33342609	3	19	theme	21-33	405:409	arg1	%					410:410	%	410:410	%	410:410	They contained 21-33% uronic acids (UA) with low degree of methylation and 0.5-1.2 M ratios of neutral sugars to UA.
33342609	4	20	theme	High	507:510	arg1	level					522:526	High arabinose level	507:526	High arabinose level	507:526	High arabinose level suggests that long arabinan side-chains maintain cell wall flexibility in water deficit.
33342609	7	21	theme	gel	768:770	arg1	networks					778:785	"weak gel"-type networks	762:785	"weak gel"-type networks	762:785	At 2.0% w/v, pectin fractions developed "weak gel"-type networks with Ca2+, while arabinoxylans generated "dilute solutions".
33342609	3	22	theme	%	410:410	arg1	UA					426:427	UA	426:427	UA	426:427	They contained 21-33% uronic acids (UA) with low degree of methylation and 0.5-1.2 M ratios of neutral sugars to UA.
33342609	3	22	theme	%	410:410	arg1	acids					419:423	21-33% uronic acids	405:423	21-33% uronic acids (UA) with low degree of methylation	405:459	They contained 21-33% uronic acids (UA) with low degree of methylation and 0.5-1.2 M ratios of neutral sugars to UA.
33342609	8	23	theme	swelling	954:961	arg1	capacity					963:970	low water swelling capacity	944:970	low water swelling capacity (3.6 mL/g)	944:981	Cellulose (28%) and lignin (45.1%) were the main biopolymers in the final residue, which showed low water swelling capacity (3.6 mL/g) due to lignin, increasing when arabinoxylans were also present.
33342609	8	23	theme	swelling	954:961	arg1	3.6 mL/g					973:980	3.6 mL/g	973:980	3.6 mL/g	973:980	Cellulose (28%) and lignin (45.1%) were the main biopolymers in the final residue, which showed low water swelling capacity (3.6 mL/g) due to lignin, increasing when arabinoxylans were also present.
33342609	8	24	theme	main	892:895	arg1	lignin					868:873	lignin	868:873	lignin (45.1%)	868:881	Cellulose (28%) and lignin (45.1%) were the main biopolymers in the final residue, which showed low water swelling capacity (3.6 mL/g) due to lignin, increasing when arabinoxylans were also present.
33342609	8	24	theme	main	892:895	arg1	Cellulose					848:856	Cellulose	848:856	Cellulose (28%)	848:862	Cellulose (28%) and lignin (45.1%) were the main biopolymers in the final residue, which showed low water swelling capacity (3.6 mL/g) due to lignin, increasing when arabinoxylans were also present.
33342609	8	24	theme	main	892:895	arg1	biopolymers					897:907	the main biopolymers	888:907	the main biopolymers in the final residue, which showed low water swelling capacity (3.6 mL/g) due to lignin, increasing when arabinoxylans were also present	888:1044	Cellulose (28%) and lignin (45.1%) were the main biopolymers in the final residue, which showed low water swelling capacity (3.6 mL/g) due to lignin, increasing when arabinoxylans were also present.
33342609	8	25	theme	water	948:952	arg1	capacity					963:970	low water swelling capacity	944:970	low water swelling capacity (3.6 mL/g)	944:981	Cellulose (28%) and lignin (45.1%) were the main biopolymers in the final residue, which showed low water swelling capacity (3.6 mL/g) due to lignin, increasing when arabinoxylans were also present.
33342609	8	25	theme	water	948:952	arg1	3.6 mL/g					973:980	3.6 mL/g	973:980	3.6 mL/g	973:980	Cellulose (28%) and lignin (45.1%) were the main biopolymers in the final residue, which showed low water swelling capacity (3.6 mL/g) due to lignin, increasing when arabinoxylans were also present.
33342609	3	26	theme	0.5-1.2 M	465:473	arg1	ratios					475:480	0.5-1.2 M ratios	465:480	0.5-1.2 M ratios of neutral sugars to UA	465:504	They contained 21-33% uronic acids (UA) with low degree of methylation and 0.5-1.2 M ratios of neutral sugars to UA.
33342609	10	27	theme	food	1173:1176	arg1	applications					1178:1189	food applications	1173:1189	food applications	1173:1189	Salicornia is a source of biopolymers and antioxidants potentially useful for food applications.
33342609	1	28	theme	neei	121:124	arg1	halophyte					126:134	Salicornia neei halophyte	110:134	Salicornia neei halophyte	110:134	Salicornia neei halophyte extends in Argentina seashores.
33342609	2	29	theme	potential	180:188	arg1	applications					190:201	potential applications	180:201	potential applications	180:201	To envisage potential applications, cell wall sequential extraction performed on dry plant yielded 1.1, 2.4, 0.3 and 0.9% of pectin fractions respectively extracted by room temperature water, 90 °C-water, CDTA and Na2CO3.
33342609	2	30	theme	dry	249:251	arg1	plant					253:257	dry plant	249:257	dry plant	249:257	To envisage potential applications, cell wall sequential extraction performed on dry plant yielded 1.1, 2.4, 0.3 and 0.9% of pectin fractions respectively extracted by room temperature water, 90 °C-water, CDTA and Na2CO3.
33342609	7	31	theme	weak	763:766	arg1	networks					778:785	"weak gel"-type networks	762:785	"weak gel"-type networks	762:785	At 2.0% w/v, pectin fractions developed "weak gel"-type networks with Ca2+, while arabinoxylans generated "dilute solutions".
33342609	7	32	theme	%	728:728	arg1	w/v					730:732	2.0% w/v	725:732	2.0% w/v	725:732	At 2.0% w/v, pectin fractions developed "weak gel"-type networks with Ca2+, while arabinoxylans generated "dilute solutions".
33342609	4	33	theme	water	602:606	arg1	deficit					608:614	water deficit	602:614	water deficit	602:614	High arabinose level suggests that long arabinan side-chains maintain cell wall flexibility in water deficit.
33342609	0	34	theme	wall	47:50	arg1	composition					52:62	Cell wall composition	42:62	Cell wall composition	42:62	Perennial halophyte Salicornia neei Lag.: Cell wall composition and functional properties of its biopolymers.
33342609	7	35	theme	pectin	735:740	arg1	fractions					742:750	pectin fractions	735:750	pectin fractions	735:750	At 2.0% w/v, pectin fractions developed "weak gel"-type networks with Ca2+, while arabinoxylans generated "dilute solutions".
33342609	1	36	theme	Salicornia	110:119	arg1	halophyte					126:134	Salicornia neei halophyte	110:134	Salicornia neei halophyte	110:134	Salicornia neei halophyte extends in Argentina seashores.
33342609	0	37	theme	Cell	42:45	arg1	composition					52:62	Cell wall composition	42:62	Cell wall composition	42:62	Perennial halophyte Salicornia neei Lag.: Cell wall composition and functional properties of its biopolymers.
33342609	2	38	theme	sequential	214:223	arg1	extraction					225:234	cell wall sequential extraction	204:234	cell wall sequential extraction performed on dry plant	204:257	To envisage potential applications, cell wall sequential extraction performed on dry plant yielded 1.1, 2.4, 0.3 and 0.9% of pectin fractions respectively extracted by room temperature water, 90 °C-water, CDTA and Na2CO3.
33342609	0	39	dep	Lag.	36:39	arg1	composition					52:62	Cell wall composition	42:62	Cell wall composition	42:62	Perennial halophyte Salicornia neei Lag.: Cell wall composition and functional properties of its biopolymers.
33342609	0	39	dep	Lag.	36:39	arg1	properties					79:88	functional properties	68:88	functional properties of its biopolymers	68:107	Perennial halophyte Salicornia neei Lag.: Cell wall composition and functional properties of its biopolymers.
33342609	3	40	theme	methylation	449:459	arg1	degree					439:444	low degree	435:444	low degree of methylation	435:459	They contained 21-33% uronic acids (UA) with low degree of methylation and 0.5-1.2 M ratios of neutral sugars to UA.
33342609	7	41	theme	2.0	725:727	arg1	%					728:728	%	728:728	%	728:728	At 2.0% w/v, pectin fractions developed "weak gel"-type networks with Ca2+, while arabinoxylans generated "dilute solutions".
33342609	2	42	theme	wall	209:212	arg1	extraction					225:234	cell wall sequential extraction	204:234	cell wall sequential extraction performed on dry plant	204:257	To envisage potential applications, cell wall sequential extraction performed on dry plant yielded 1.1, 2.4, 0.3 and 0.9% of pectin fractions respectively extracted by room temperature water, 90 °C-water, CDTA and Na2CO3.
33342609	3	43	theme	low	435:437	arg1	degree					439:444	low degree	435:444	low degree of methylation	435:459	They contained 21-33% uronic acids (UA) with low degree of methylation and 0.5-1.2 M ratios of neutral sugars to UA.
33342609	5	44	contain	contained	632:640	arg1	Fractions					617:625	Fractions	617:625	Fractions	617:625	Fractions also contained 10-36% of proteins.
33342609	5	44	contain	contained	632:640	arg2	proteins					652:659	proteins	652:659	proteins	652:659	Fractions also contained 10-36% of proteins.
33342609	5	44	contain	contained	632:640	arg2	%					647:647	10-36%	642:647	10-36% of proteins	642:659	Fractions also contained 10-36% of proteins.
33342609	3	45	with	acids	419:423	arg1	degree					439:444	low degree	435:444	low degree of methylation	435:459	They contained 21-33% uronic acids (UA) with low degree of methylation and 0.5-1.2 M ratios of neutral sugars to UA.
33342609	2	46	theme	cell	204:207	arg1	extraction					225:234	cell wall sequential extraction	204:234	cell wall sequential extraction performed on dry plant	204:257	To envisage potential applications, cell wall sequential extraction performed on dry plant yielded 1.1, 2.4, 0.3 and 0.9% of pectin fractions respectively extracted by room temperature water, 90 °C-water, CDTA and Na2CO3.
33342609	2	47	theme	fractions	300:308	arg1	%					288:288	1.1, 2.4, 0.3 and 0.9%	267:288	1.1, 2.4, 0.3 and 0.9% of pectin fractions respectively extracted by room temperature water, 90 °C-water, CDTA and Na2CO3	267:387	To envisage potential applications, cell wall sequential extraction performed on dry plant yielded 1.1, 2.4, 0.3 and 0.9% of pectin fractions respectively extracted by room temperature water, 90 °C-water, CDTA and Na2CO3.
33342609	2	47	theme	fractions	300:308	arg1	fractions					300:308	pectin fractions	293:308	pectin fractions respectively extracted by room temperature water, 90 °C-water, CDTA and Na2CO3	293:387	To envisage potential applications, cell wall sequential extraction performed on dry plant yielded 1.1, 2.4, 0.3 and 0.9% of pectin fractions respectively extracted by room temperature water, 90 °C-water, CDTA and Na2CO3.
33342609	10	48	theme	antioxidants	1137:1148	arg1	Salicornia					1095:1104	Salicornia	1095:1104	Salicornia	1095:1104	Salicornia is a source of biopolymers and antioxidants potentially useful for food applications.
33342609	10	48	theme	antioxidants	1137:1148	arg1	source					1111:1116	a source	1109:1116	a source of biopolymers and antioxidants potentially useful for food applications	1109:1189	Salicornia is a source of biopolymers and antioxidants potentially useful for food applications.
33342609	8	49	from	biopolymers	897:907	arg1	residue					922:928	the final residue	912:928	the final residue	912:928	Cellulose (28%) and lignin (45.1%) were the main biopolymers in the final residue, which showed low water swelling capacity (3.6 mL/g) due to lignin, increasing when arabinoxylans were also present.
33342609	7	50	theme	dilute	829:834	arg1	solutions					836:844	"dilute solutions	828:844	"dilute solutions"	828:845	At 2.0% w/v, pectin fractions developed "weak gel"-type networks with Ca2+, while arabinoxylans generated "dilute solutions".
33342609	4	51	theme	wall	582:585	arg1	flexibility					587:597	cell wall flexibility	577:597	cell wall flexibility	577:597	High arabinose level suggests that long arabinan side-chains maintain cell wall flexibility in water deficit.
33342609	1	52	theme	Argentina	147:155	arg1	seashores					157:165	Argentina seashores	147:165	Argentina seashores	147:165	Salicornia neei halophyte extends in Argentina seashores.
33342609	10	53	theme	biopolymers	1121:1131	arg1	Salicornia					1095:1104	Salicornia	1095:1104	Salicornia	1095:1104	Salicornia is a source of biopolymers and antioxidants potentially useful for food applications.
33342609	10	53	theme	biopolymers	1121:1131	arg1	source					1111:1116	a source	1109:1116	a source of biopolymers and antioxidants potentially useful for food applications	1109:1189	Salicornia is a source of biopolymers and antioxidants potentially useful for food applications.
33342609	4	54	theme	cell	577:580	arg1	flexibility					587:597	cell wall flexibility	577:597	cell wall flexibility	577:597	High arabinose level suggests that long arabinan side-chains maintain cell wall flexibility in water deficit.
32407945	8	0	theme	sample	1253:1258	arg1	A					1260:1260	the sample A	1249:1260	the sample A (Mw: 4,818,700 g/mol)	1249:1282	Furthermore, the immunomodulatory effect of lentinan sample B (Mw: 650,700 g/mol) was superior than that of the sample A (Mw: 4,818,700 g/mol).
32407945	8	0	theme	sample	1253:1258	arg1	Mw					1263:1264	Mw	1263:1264	Mw	1263:1264	Furthermore, the immunomodulatory effect of lentinan sample B (Mw: 650,700 g/mol) was superior than that of the sample A (Mw: 4,818,700 g/mol).
32407945	9	1	theme	necessary	1334:1342	arg1	index					1344:1348	a necessary index	1332:1348	a necessary index for quality control of lentinan to ensure stability and effectiveness of the production process	1332:1444	It was noted that the Mw should be detected as a necessary index for quality control of lentinan to ensure stability and effectiveness of the production process.
32407945	9	1	theme	necessary	1334:1342	arg1	Mw					1307:1308	the Mw	1303:1308	the Mw	1303:1308	It was noted that the Mw should be detected as a necessary index for quality control of lentinan to ensure stability and effectiveness of the production process.
32407945	4	2	theme	batch-to-batch	494:507	arg1	consistency					509:519	the batch-to-batch consistency	490:519	the batch-to-batch consistency of two lentinan samples	490:543	The results showed that the batch-to-batch consistency of two lentinan samples was satisfactory, indicating the stability of production process of lentinan.
32407945	4	2	theme	batch-to-batch	494:507	arg1	satisfactory					549:560	satisfactory	549:560	satisfactory	549:560	The results showed that the batch-to-batch consistency of two lentinan samples was satisfactory, indicating the stability of production process of lentinan.
32407945	5	3	theme	chemical	645:652	arg1	similar					742:748	similar	742:748	similar	742:748	However, although the chemical composition and triple-helical conformation (THC) of the tested samples were relatively similar, their Mw, polydispersity index (PDI), and Rgz remarkably varied due to different production processes.
32407945	5	3	theme	chemical	645:652	arg1	composition					654:664	the chemical composition	641:664	the chemical composition	641:664	However, although the chemical composition and triple-helical conformation (THC) of the tested samples were relatively similar, their Mw, polydispersity index (PDI), and Rgz remarkably varied due to different production processes.
32407945	7	4	theme	Meanwhile	962:970	arg1	samples					982:988	Meanwhile, lentinan samples	962:988	Meanwhile, lentinan samples	962:988	Meanwhile, lentinan samples could improve the spleen and thymus indices, promote the proliferation of lymphocytes and adjust for the percentages of CD4+ and CD8+ T cells in vivo.
32407945	1	5	theme	malignant	146:154	arg1	tumors					156:161	malignant tumors	146:161	malignant tumors in clinical practice	146:182	Lentinan is widely used as a therapeutic agent for treatment of malignant tumors in clinical practice.
32407945	5	6	theme	samples	718:724	arg1	THC					699:701	THC	699:701	THC	699:701	However, although the chemical composition and triple-helical conformation (THC) of the tested samples were relatively similar, their Mw, polydispersity index (PDI), and Rgz remarkably varied due to different production processes.
32407945	5	6	theme	samples	718:724	arg1	composition					654:664	the chemical composition	641:664	the chemical composition	641:664	However, although the chemical composition and triple-helical conformation (THC) of the tested samples were relatively similar, their Mw, polydispersity index (PDI), and Rgz remarkably varied due to different production processes.
32407945	5	6	theme	samples	718:724	arg1	conformation					685:696	triple-helical conformation	670:696	triple-helical conformation (THC)	670:702	However, although the chemical composition and triple-helical conformation (THC) of the tested samples were relatively similar, their Mw, polydispersity index (PDI), and Rgz remarkably varied due to different production processes.
32407945	5	6	theme	samples	718:724	arg1	similar					742:748	similar	742:748	similar	742:748	However, although the chemical composition and triple-helical conformation (THC) of the tested samples were relatively similar, their Mw, polydispersity index (PDI), and Rgz remarkably varied due to different production processes.
32407945	3	7	theme	immune	344:349	arg1	activity					351:358	its immune activity	340:358	its immune activity	340:358	In this study, the correlation between the structure of lentinan and its immune activity was investigated to assess the function of key parameters that can influence quality control of lentinan.
32407945	9	8	theme	process	1438:1444	arg1	stability					1392:1400	stability	1392:1400	stability	1392:1400	It was noted that the Mw should be detected as a necessary index for quality control of lentinan to ensure stability and effectiveness of the production process.
32407945	9	8	theme	process	1438:1444	arg1	effectiveness					1406:1418	effectiveness	1406:1418	effectiveness	1406:1418	It was noted that the Mw should be detected as a necessary index for quality control of lentinan to ensure stability and effectiveness of the production process.
32407945	7	9	theme	CD4+	1110:1113	arg1	cells					1126:1130	CD4+ and CD8+ T cells	1110:1130	CD4+ and CD8+ T cells	1110:1130	Meanwhile, lentinan samples could improve the spleen and thymus indices, promote the proliferation of lymphocytes and adjust for the percentages of CD4+ and CD8+ T cells in vivo.
32407945	9	10	theme	quality	1354:1360	arg1	control					1362:1368	quality control	1354:1368	quality control of lentinan to ensure stability and effectiveness of the production process	1354:1444	It was noted that the Mw should be detected as a necessary index for quality control of lentinan to ensure stability and effectiveness of the production process.
32407945	3	11	theme	lentinan	327:334	arg1	structure					314:322	the structure	310:322	the structure of lentinan	310:334	In this study, the correlation between the structure of lentinan and its immune activity was investigated to assess the function of key parameters that can influence quality control of lentinan.
32407945	3	11	theme	lentinan	327:334	arg1	activity					351:358	its immune activity	340:358	its immune activity	340:358	In this study, the correlation between the structure of lentinan and its immune activity was investigated to assess the function of key parameters that can influence quality control of lentinan.
32407945	6	12	theme	immunomodulatory	863:878	arg1	assay					880:884	In vitro immunomodulatory assay	854:884	In vitro immunomodulatory assay	854:884	In vitro immunomodulatory assay reflects that lentinan could stimulate the macrophages phagocytic capacity.
32407945	1	13	from	treatment	133:141	arg1	practice					175:182	clinical practice	166:182	clinical practice	166:182	Lentinan is widely used as a therapeutic agent for treatment of malignant tumors in clinical practice.
32407945	1	14	theme	clinical	166:173	arg1	practice					175:182	clinical practice	166:182	clinical practice	166:182	Lentinan is widely used as a therapeutic agent for treatment of malignant tumors in clinical practice.
32407945	1	15	theme	tumors	156:161	arg1	treatment					133:141	treatment	133:141	treatment of malignant tumors in clinical practice	133:182	Lentinan is widely used as a therapeutic agent for treatment of malignant tumors in clinical practice.
32407945	7	16	theme	cells	1126:1130	arg1	percentages					1095:1105	the percentages	1091:1105	the percentages of CD4+ and CD8+ T cells	1091:1130	Meanwhile, lentinan samples could improve the spleen and thymus indices, promote the proliferation of lymphocytes and adjust for the percentages of CD4+ and CD8+ T cells in vivo.
32407945	8	17	theme	B	1201:1201	arg1	effect					1175:1180	the immunomodulatory effect	1154:1180	the immunomodulatory effect of lentinan sample B (Mw: 650,700 g/mol)	1154:1221	Furthermore, the immunomodulatory effect of lentinan sample B (Mw: 650,700 g/mol) was superior than that of the sample A (Mw: 4,818,700 g/mol).
32407945	8	17	theme	B	1201:1201	arg1	superior					1227:1234	superior	1227:1234	superior	1227:1234	Furthermore, the immunomodulatory effect of lentinan sample B (Mw: 650,700 g/mol) was superior than that of the sample A (Mw: 4,818,700 g/mol).
32407945	6	18	dep	In	854:855	arg1	vitro					857:861	vitro	857:861	vitro	857:861	In vitro immunomodulatory assay reflects that lentinan could stimulate the macrophages phagocytic capacity.
32407945	5	19	theme	production	832:841	arg1	processes					843:851	different production processes	822:851	different production processes	822:851	However, although the chemical composition and triple-helical conformation (THC) of the tested samples were relatively similar, their Mw, polydispersity index (PDI), and Rgz remarkably varied due to different production processes.
32407945	6	20	theme	In	854:855	arg1	assay					880:884	In vitro immunomodulatory assay	854:884	In vitro immunomodulatory assay	854:884	In vitro immunomodulatory assay reflects that lentinan could stimulate the macrophages phagocytic capacity.
32407945	3	21	theme	key	403:405	arg1	parameters					407:416	key parameters	403:416	key parameters that can influence quality control of lentinan	403:463	In this study, the correlation between the structure of lentinan and its immune activity was investigated to assess the function of key parameters that can influence quality control of lentinan.
32407945	0	22	theme	Quality	0:6	arg1	control					8:14	Quality control	0:14	Quality control	0:14	Quality control and immunological activity of lentinan samples produced in China.
32407945	3	23	theme	parameters	407:416	arg1	function					391:398	the function	387:398	the function of key parameters that can influence quality control of lentinan	387:463	In this study, the correlation between the structure of lentinan and its immune activity was investigated to assess the function of key parameters that can influence quality control of lentinan.
32407945	0	24	theme	immunological	20:32	arg1	activity					34:41	immunological activity	20:41	immunological activity	20:41	Quality control and immunological activity of lentinan samples produced in China.
32407945	4	25	theme	samples	537:543	arg1	consistency					509:519	the batch-to-batch consistency	490:519	the batch-to-batch consistency of two lentinan samples	490:543	The results showed that the batch-to-batch consistency of two lentinan samples was satisfactory, indicating the stability of production process of lentinan.
32407945	4	25	theme	samples	537:543	arg1	satisfactory					549:560	satisfactory	549:560	satisfactory	549:560	The results showed that the batch-to-batch consistency of two lentinan samples was satisfactory, indicating the stability of production process of lentinan.
32407945	7	26	theme	lymphocytes	1064:1074	arg1	proliferation					1047:1059	the proliferation	1043:1059	the proliferation of lymphocytes	1043:1074	Meanwhile, lentinan samples could improve the spleen and thymus indices, promote the proliferation of lymphocytes and adjust for the percentages of CD4+ and CD8+ T cells in vivo.
32407945	8	27	dep	Mw	1263:1264	arg1	4,818,700 g/mol					1267:1281	4,818,700 g/mol	1267:1281	4,818,700 g/mol	1267:1281	Furthermore, the immunomodulatory effect of lentinan sample B (Mw: 650,700 g/mol) was superior than that of the sample A (Mw: 4,818,700 g/mol).
32407945	9	28	theme	production	1427:1436	arg1	process					1438:1444	the production process	1423:1444	the production process	1423:1444	It was noted that the Mw should be detected as a necessary index for quality control of lentinan to ensure stability and effectiveness of the production process.
32407945	5	29	theme	polydispersity	761:774	arg1	PDI					783:785	PDI	783:785	PDI	783:785	However, although the chemical composition and triple-helical conformation (THC) of the tested samples were relatively similar, their Mw, polydispersity index (PDI), and Rgz remarkably varied due to different production processes.
32407945	5	29	theme	polydispersity	761:774	arg1	index					776:780	polydispersity index	761:780	polydispersity index (PDI)	761:786	However, although the chemical composition and triple-helical conformation (THC) of the tested samples were relatively similar, their Mw, polydispersity index (PDI), and Rgz remarkably varied due to different production processes.
32407945	8	30	dep	Mw	1204:1205	arg1	650,700 g/mol					1208:1220	650,700 g/mol	1208:1220	650,700 g/mol	1208:1220	Furthermore, the immunomodulatory effect of lentinan sample B (Mw: 650,700 g/mol) was superior than that of the sample A (Mw: 4,818,700 g/mol).
32407945	5	31	theme	different	822:830	arg1	processes					843:851	different production processes	822:851	different production processes	822:851	However, although the chemical composition and triple-helical conformation (THC) of the tested samples were relatively similar, their Mw, polydispersity index (PDI), and Rgz remarkably varied due to different production processes.
32407945	6	32	theme	phagocytic	941:950	arg1	capacity					952:959	the macrophages phagocytic capacity	925:959	the macrophages phagocytic capacity	925:959	In vitro immunomodulatory assay reflects that lentinan could stimulate the macrophages phagocytic capacity.
32407945	5	33	theme	tested	711:716	arg1	samples					718:724	the tested samples	707:724	the tested samples	707:724	However, although the chemical composition and triple-helical conformation (THC) of the tested samples were relatively similar, their Mw, polydispersity index (PDI), and Rgz remarkably varied due to different production processes.
32407945	8	34	theme	lentinan	1185:1192	arg1	B					1201:1201	lentinan sample B	1185:1201	lentinan sample B (Mw: 650,700 g/mol)	1185:1221	Furthermore, the immunomodulatory effect of lentinan sample B (Mw: 650,700 g/mol) was superior than that of the sample A (Mw: 4,818,700 g/mol).
32407945	8	34	theme	lentinan	1185:1192	arg1	Mw					1204:1205	Mw	1204:1205	Mw	1204:1205	Furthermore, the immunomodulatory effect of lentinan sample B (Mw: 650,700 g/mol) was superior than that of the sample A (Mw: 4,818,700 g/mol).
32407945	3	35	theme	quality	437:443	arg1	control					445:451	quality control	437:451	quality control of lentinan	437:463	In this study, the correlation between the structure of lentinan and its immune activity was investigated to assess the function of key parameters that can influence quality control of lentinan.
32407945	0	36	theme	samples	55:61	arg1	control					8:14	Quality control	0:14	Quality control	0:14	Quality control and immunological activity of lentinan samples produced in China.
32407945	0	36	theme	samples	55:61	arg1	activity					34:41	immunological activity	20:41	immunological activity	20:41	Quality control and immunological activity of lentinan samples produced in China.
32407945	6	37	theme	macrophages	929:939	arg1	capacity					952:959	the macrophages phagocytic capacity	925:959	the macrophages phagocytic capacity	925:959	In vitro immunomodulatory assay reflects that lentinan could stimulate the macrophages phagocytic capacity.
32407945	5	38	theme	triple-helical	670:683	arg1	THC					699:701	THC	699:701	THC	699:701	However, although the chemical composition and triple-helical conformation (THC) of the tested samples were relatively similar, their Mw, polydispersity index (PDI), and Rgz remarkably varied due to different production processes.
32407945	5	38	theme	triple-helical	670:683	arg1	conformation					685:696	triple-helical conformation	670:696	triple-helical conformation (THC)	670:702	However, although the chemical composition and triple-helical conformation (THC) of the tested samples were relatively similar, their Mw, polydispersity index (PDI), and Rgz remarkably varied due to different production processes.
32407945	2	39	theme	lentinan	211:218	arg1	structure					198:206	The chemical structure	185:206	The chemical structure of lentinan	185:218	The chemical structure of lentinan is highly associated with its biological activity.
32407945	7	40	theme	thymus	1019:1024	arg1	indices					1026:1032	the spleen and thymus indices	1004:1032	the spleen and thymus indices	1004:1032	Meanwhile, lentinan samples could improve the spleen and thymus indices, promote the proliferation of lymphocytes and adjust for the percentages of CD4+ and CD8+ T cells in vivo.
32407945	1	41	used	used	101:104	arg2	agent					123:127	a therapeutic agent	109:127	a therapeutic agent for treatment of malignant tumors in clinical practice	109:182	Lentinan is widely used as a therapeutic agent for treatment of malignant tumors in clinical practice.
32407945	1	41	used	used	101:104	arg2	Lentinan					82:89	Lentinan	82:89	Lentinan	82:89	Lentinan is widely used as a therapeutic agent for treatment of malignant tumors in clinical practice.
32407945	0	42	theme	lentinan	46:53	arg1	samples					55:61	lentinan samples	46:61	lentinan samples	46:61	Quality control and immunological activity of lentinan samples produced in China.
32407945	4	43	theme	lentinan	528:535	arg1	samples					537:543	two lentinan samples	524:543	two lentinan samples	524:543	The results showed that the batch-to-batch consistency of two lentinan samples was satisfactory, indicating the stability of production process of lentinan.
32407945	3	44	theme	lentinan	456:463	arg1	control					445:451	quality control	437:451	quality control of lentinan	437:463	In this study, the correlation between the structure of lentinan and its immune activity was investigated to assess the function of key parameters that can influence quality control of lentinan.
32407945	7	45	theme	CD8+	1119:1122	arg1	cells					1126:1130	CD4+ and CD8+ T cells	1110:1130	CD4+ and CD8+ T cells	1110:1130	Meanwhile, lentinan samples could improve the spleen and thymus indices, promote the proliferation of lymphocytes and adjust for the percentages of CD4+ and CD8+ T cells in vivo.
32407945	8	46	theme	sample	1194:1199	arg1	B					1201:1201	lentinan sample B	1185:1201	lentinan sample B (Mw: 650,700 g/mol)	1185:1221	Furthermore, the immunomodulatory effect of lentinan sample B (Mw: 650,700 g/mol) was superior than that of the sample A (Mw: 4,818,700 g/mol).
32407945	8	46	theme	sample	1194:1199	arg1	Mw					1204:1205	Mw	1204:1205	Mw	1204:1205	Furthermore, the immunomodulatory effect of lentinan sample B (Mw: 650,700 g/mol) was superior than that of the sample A (Mw: 4,818,700 g/mol).
32407945	2	47	theme	chemical	189:196	arg1	structure					198:206	The chemical structure	185:206	The chemical structure of lentinan	185:218	The chemical structure of lentinan is highly associated with its biological activity.
32407945	2	48	theme	biological	250:259	arg1	activity					261:268	its biological activity	246:268	its biological activity	246:268	The chemical structure of lentinan is highly associated with its biological activity.
32407945	7	49	theme	spleen	1008:1013	arg1	indices					1026:1032	the spleen and thymus indices	1004:1032	the spleen and thymus indices	1004:1032	Meanwhile, lentinan samples could improve the spleen and thymus indices, promote the proliferation of lymphocytes and adjust for the percentages of CD4+ and CD8+ T cells in vivo.
32407945	1	50	theme	therapeutic	111:121	arg1	agent					123:127	a therapeutic agent	109:127	a therapeutic agent for treatment of malignant tumors in clinical practice	109:182	Lentinan is widely used as a therapeutic agent for treatment of malignant tumors in clinical practice.
32407945	1	50	theme	therapeutic	111:121	arg1	Lentinan					82:89	Lentinan	82:89	Lentinan	82:89	Lentinan is widely used as a therapeutic agent for treatment of malignant tumors in clinical practice.
32407945	4	51	theme	process	602:608	arg1	stability					578:586	the stability	574:586	the stability of production process of lentinan	574:620	The results showed that the batch-to-batch consistency of two lentinan samples was satisfactory, indicating the stability of production process of lentinan.
32407945	8	52	theme	immunomodulatory	1158:1173	arg1	effect					1175:1180	the immunomodulatory effect	1154:1180	the immunomodulatory effect of lentinan sample B (Mw: 650,700 g/mol)	1154:1221	Furthermore, the immunomodulatory effect of lentinan sample B (Mw: 650,700 g/mol) was superior than that of the sample A (Mw: 4,818,700 g/mol).
32407945	8	52	theme	immunomodulatory	1158:1173	arg1	superior					1227:1234	superior	1227:1234	superior	1227:1234	Furthermore, the immunomodulatory effect of lentinan sample B (Mw: 650,700 g/mol) was superior than that of the sample A (Mw: 4,818,700 g/mol).
32407945	9	53	theme	lentinan	1373:1380	arg1	control					1362:1368	quality control	1354:1368	quality control of lentinan to ensure stability and effectiveness of the production process	1354:1444	It was noted that the Mw should be detected as a necessary index for quality control of lentinan to ensure stability and effectiveness of the production process.
32407945	7	54	dep	Meanwhile	962:970	arg1	lentinan					973:980	lentinan	973:980	lentinan	973:980	Meanwhile, lentinan samples could improve the spleen and thymus indices, promote the proliferation of lymphocytes and adjust for the percentages of CD4+ and CD8+ T cells in vivo.
32407945	4	55	theme	production	591:600	arg1	process					602:608	production process	591:608	production process of lentinan	591:620	The results showed that the batch-to-batch consistency of two lentinan samples was satisfactory, indicating the stability of production process of lentinan.
32407945	1	56	from	practice	175:182	arg1	treatment					133:141	treatment	133:141	treatment of malignant tumors in clinical practice	133:182	Lentinan is widely used as a therapeutic agent for treatment of malignant tumors in clinical practice.
32407945	4	57	theme	lentinan	613:620	arg1	process					602:608	production process	591:608	production process of lentinan	591:620	The results showed that the batch-to-batch consistency of two lentinan samples was satisfactory, indicating the stability of production process of lentinan.
32407945	7	58	theme	T	1124:1124	arg1	cells					1126:1130	CD4+ and CD8+ T cells	1110:1130	CD4+ and CD8+ T cells	1110:1130	Meanwhile, lentinan samples could improve the spleen and thymus indices, promote the proliferation of lymphocytes and adjust for the percentages of CD4+ and CD8+ T cells in vivo.
32407945	1	59	from	tumors	156:161	arg1	practice					175:182	clinical practice	166:182	clinical practice	166:182	Lentinan is widely used as a therapeutic agent for treatment of malignant tumors in clinical practice.
32344092	1	0	theme	PVA	331:333	arg1	film					372:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	To avoid complex and toxic chemical cross-linking reaction, cellulose nanofibrils (CNF) was used to enhance the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film.
32344092	6	1	theme	green	1167:1171	arg1	approaches					1198:1207	The green and flexible fabrication approaches	1163:1207	The green and flexible fabrication approaches	1163:1207	The green and flexible fabrication approaches will be helpful to build up eco-friendly BN composites with wide applications in thermal management of electronics.
32344092	6	1	theme	green	1167:1171	arg1	helpful					1217:1223	helpful	1217:1223	helpful	1217:1223	The green and flexible fabrication approaches will be helpful to build up eco-friendly BN composites with wide applications in thermal management of electronics.
32344092	1	2	theme	toxic	165:169	arg1	reaction					194:201	toxic chemical cross-linking reaction	165:201	toxic chemical cross-linking reaction	165:201	To avoid complex and toxic chemical cross-linking reaction, cellulose nanofibrils (CNF) was used to enhance the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film.
32344092	5	3	theme	film	988:991	arg1	CNF					937:939	CNF	937:939	CNF	937:939	Furthermore, the rigidity of composite film was strengthened by incorporating CNF and LNP, the tensile strength of the composite film showed the highest value of 35.0 MPa with 4 wt% CNF and 0.01 g LNP, while this of BNNS/PVA/LNP film and BNNS/PVA/CNF film was only 30.66 MPa and 30.62 MPa, respectively.
32344092	5	3	theme	film	988:991	arg1	strength					962:969	the tensile strength	950:969	the tensile strength of the composite film showed the highest value of 35.0 MPa with 4 wt% CNF and 0.01 g LNP, while this of BNNS/PVA/LNP film and BNNS/PVA/CNF film was only 30.66 MPa and 30.62 MPa, respectively	950:1160	Furthermore, the rigidity of composite film was strengthened by incorporating CNF and LNP, the tensile strength of the composite film showed the highest value of 35.0 MPa with 4 wt% CNF and 0.01 g LNP, while this of BNNS/PVA/LNP film and BNNS/PVA/CNF film was only 30.66 MPa and 30.62 MPa, respectively.
32344092	2	4	theme	tensile	455:461	arg1	strength					463:470	tensile strength	455:470	tensile strength	455:470	The results showed that CNF and LNP had synergistic actions on enhancing the tensile strength, thermal conductivity and stability of the film.
32344092	5	5	theme	35.0 MPa	1021:1028	arg1	value					1012:1016	the highest value	1000:1016	the highest value of 35.0 MPa	1000:1028	Furthermore, the rigidity of composite film was strengthened by incorporating CNF and LNP, the tensile strength of the composite film showed the highest value of 35.0 MPa with 4 wt% CNF and 0.01 g LNP, while this of BNNS/PVA/LNP film and BNNS/PVA/CNF film was only 30.66 MPa and 30.62 MPa, respectively.
32344092	3	6	from	loading	714:720	arg1	increase					679:686	an increase	676:686	an increase of 22.2% at the same BNNS loading	676:720	The 4 wt% CNF-reinforced composites exhibited the higher through-plane thermal conductivity (up to 1.76 W/mK) than that without CNF (1.44 W/mK), revealing an increase of 22.2% at the same BNNS loading.
32344092	3	6	from	loading	714:720	arg1	%					695:695	22.2%	691:695	22.2% at the same BNNS loading	691:720	The 4 wt% CNF-reinforced composites exhibited the higher through-plane thermal conductivity (up to 1.76 W/mK) than that without CNF (1.44 W/mK), revealing an increase of 22.2% at the same BNNS loading.
32344092	1	7	theme	chemical	171:178	arg1	reaction					194:201	toxic chemical cross-linking reaction	165:201	toxic chemical cross-linking reaction	165:201	To avoid complex and toxic chemical cross-linking reaction, cellulose nanofibrils (CNF) was used to enhance the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film.
32344092	0	8	theme	nanofibrils	106:116	arg1	actions					85:91	the synergistic actions	69:91	the synergistic actions of cellulose nanofibrils and lignin nanoparticles	69:141	BNNS/PVA bilayer composite film with multiple-improved properties by the synergistic actions of cellulose nanofibrils and lignin nanoparticles.
32344092	1	9	theme	/lignin	335:341	arg1	film					372:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	To avoid complex and toxic chemical cross-linking reaction, cellulose nanofibrils (CNF) was used to enhance the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film.
32344092	5	10	theme	BNNS/PVA/LNP	1075:1086	arg1	film					1088:1091	BNNS/PVA/LNP film	1075:1091	BNNS/PVA/LNP film	1075:1091	Furthermore, the rigidity of composite film was strengthened by incorporating CNF and LNP, the tensile strength of the composite film showed the highest value of 35.0 MPa with 4 wt% CNF and 0.01 g LNP, while this of BNNS/PVA/LNP film and BNNS/PVA/CNF film was only 30.66 MPa and 30.62 MPa, respectively.
32344092	0	11	theme	cellulose	96:104	arg1	nanofibrils					106:116	cellulose nanofibrils	96:116	cellulose nanofibrils	96:116	BNNS/PVA bilayer composite film with multiple-improved properties by the synergistic actions of cellulose nanofibrils and lignin nanoparticles.
32344092	1	12	theme	nanoparticle	343:354	arg1	film					372:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	To avoid complex and toxic chemical cross-linking reaction, cellulose nanofibrils (CNF) was used to enhance the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film.
32344092	6	13	theme	eco-friendly	1237:1248	arg1	composites					1253:1262	eco-friendly BN composites	1237:1262	eco-friendly BN composites with wide applications in thermal management of electronics	1237:1322	The green and flexible fabrication approaches will be helpful to build up eco-friendly BN composites with wide applications in thermal management of electronics.
32344092	2	14	contain	had	414:416	arg2	actions					430:436	synergistic actions	418:436	synergistic actions	418:436	The results showed that CNF and LNP had synergistic actions on enhancing the tensile strength, thermal conductivity and stability of the film.
32344092	2	14	contain	had	414:416	arg1	LNP					410:412	LNP	410:412	LNP	410:412	The results showed that CNF and LNP had synergistic actions on enhancing the tensile strength, thermal conductivity and stability of the film.
32344092	2	14	contain	had	414:416	arg1	CNF					402:404	CNF	402:404	CNF	402:404	The results showed that CNF and LNP had synergistic actions on enhancing the tensile strength, thermal conductivity and stability of the film.
32344092	1	15	theme	hexagonal	271:279	arg1	film					372:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	To avoid complex and toxic chemical cross-linking reaction, cellulose nanofibrils (CNF) was used to enhance the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film.
32344092	0	16	theme	lignin	122:127	arg1	nanoparticles					129:141	lignin nanoparticles	122:141	lignin nanoparticles	122:141	BNNS/PVA bilayer composite film with multiple-improved properties by the synergistic actions of cellulose nanofibrils and lignin nanoparticles.
32344092	1	17	theme	cross-linking	180:192	arg1	reaction					194:201	toxic chemical cross-linking reaction	165:201	toxic chemical cross-linking reaction	165:201	To avoid complex and toxic chemical cross-linking reaction, cellulose nanofibrils (CNF) was used to enhance the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film.
32344092	1	18	theme	LNP	357:359	arg1	film					372:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	To avoid complex and toxic chemical cross-linking reaction, cellulose nanofibrils (CNF) was used to enhance the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film.
32344092	4	19	theme	decomposition	746:758	arg1	%					787:787	roughly 0.6%	776:787	roughly 0.6%/oC	776:790	Meanwhile, the maximum decomposition rate (Rmax) was roughly 0.6%/oC, a decrease of 14.3% compared with the composite film without LNP.
32344092	4	19	theme	decomposition	746:758	arg1	Rmax					766:769	Rmax	766:769	Rmax	766:769	Meanwhile, the maximum decomposition rate (Rmax) was roughly 0.6%/oC, a decrease of 14.3% compared with the composite film without LNP.
32344092	4	19	theme	decomposition	746:758	arg1	rate					760:763	the maximum decomposition rate	734:763	the maximum decomposition rate (Rmax)	734:770	Meanwhile, the maximum decomposition rate (Rmax) was roughly 0.6%/oC, a decrease of 14.3% compared with the composite film without LNP.
32344092	3	20	from	increase	679:686	arg1	loading					714:720	the same BNNS loading	700:720	the same BNNS loading	700:720	The 4 wt% CNF-reinforced composites exhibited the higher through-plane thermal conductivity (up to 1.76 W/mK) than that without CNF (1.44 W/mK), revealing an increase of 22.2% at the same BNNS loading.
32344092	3	21	theme	BNNS	709:712	arg1	loading					714:720	the same BNNS loading	700:720	the same BNNS loading	700:720	The 4 wt% CNF-reinforced composites exhibited the higher through-plane thermal conductivity (up to 1.76 W/mK) than that without CNF (1.44 W/mK), revealing an increase of 22.2% at the same BNNS loading.
32344092	0	22	theme	bilayer	9:15	arg1	film					27:30	BNNS/PVA bilayer composite film	0:30	BNNS/PVA bilayer composite film with multiple-improved properties by the synergistic actions of cellulose nanofibrils and lignin nanoparticles.	0:142	BNNS/PVA bilayer composite film with multiple-improved properties by the synergistic actions of cellulose nanofibrils and lignin nanoparticles.
32344092	5	23	theme	highest	1004:1010	arg1	value					1012:1016	the highest value	1000:1016	the highest value of 35.0 MPa	1000:1028	Furthermore, the rigidity of composite film was strengthened by incorporating CNF and LNP, the tensile strength of the composite film showed the highest value of 35.0 MPa with 4 wt% CNF and 0.01 g LNP, while this of BNNS/PVA/LNP film and BNNS/PVA/CNF film was only 30.66 MPa and 30.62 MPa, respectively.
32344092	2	24	theme	thermal	473:479	arg1	conductivity					481:492	thermal conductivity	473:492	thermal conductivity	473:492	The results showed that CNF and LNP had synergistic actions on enhancing the tensile strength, thermal conductivity and stability of the film.
32344092	3	25	theme	CNF-reinforced	531:544	arg1	composites					546:555	The 4 wt% CNF-reinforced composites	521:555	The 4 wt% CNF-reinforced composites	521:555	The 4 wt% CNF-reinforced composites exhibited the higher through-plane thermal conductivity (up to 1.76 W/mK) than that without CNF (1.44 W/mK), revealing an increase of 22.2% at the same BNNS loading.
32344092	6	26	theme	flexible	1177:1184	arg1	approaches					1198:1207	The green and flexible fabrication approaches	1163:1207	The green and flexible fabrication approaches	1163:1207	The green and flexible fabrication approaches will be helpful to build up eco-friendly BN composites with wide applications in thermal management of electronics.
32344092	6	26	theme	flexible	1177:1184	arg1	helpful					1217:1223	helpful	1217:1223	helpful	1217:1223	The green and flexible fabrication approaches will be helpful to build up eco-friendly BN composites with wide applications in thermal management of electronics.
32344092	0	27	theme	BNNS/PVA	0:7	arg1	film					27:30	BNNS/PVA bilayer composite film	0:30	BNNS/PVA bilayer composite film with multiple-improved properties by the synergistic actions of cellulose nanofibrils and lignin nanoparticles.	0:142	BNNS/PVA bilayer composite film with multiple-improved properties by the synergistic actions of cellulose nanofibrils and lignin nanoparticles.
32344092	1	28	theme	boron	281:285	arg1	film					372:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	To avoid complex and toxic chemical cross-linking reaction, cellulose nanofibrils (CNF) was used to enhance the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film.
32344092	1	29	theme	cellulose	204:212	arg1	CNF					227:229	CNF	227:229	CNF	227:229	To avoid complex and toxic chemical cross-linking reaction, cellulose nanofibrils (CNF) was used to enhance the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film.
32344092	1	29	theme	cellulose	204:212	arg1	nanofibrils					214:224	cellulose nanofibrils	204:224	cellulose nanofibrils (CNF)	204:230	To avoid complex and toxic chemical cross-linking reaction, cellulose nanofibrils (CNF) was used to enhance the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film.
32344092	6	30	theme	electronics	1312:1322	arg1	management					1298:1307	thermal management	1290:1307	thermal management of electronics	1290:1322	The green and flexible fabrication approaches will be helpful to build up eco-friendly BN composites with wide applications in thermal management of electronics.
32344092	1	31	theme	nitride	287:293	arg1	film					372:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	To avoid complex and toxic chemical cross-linking reaction, cellulose nanofibrils (CNF) was used to enhance the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film.
32344092	5	32	theme	composite	978:986	arg1	film					988:991	the composite film	974:991	the composite film showed the highest value of 35.0 MPa with 4 wt% CNF and 0.01 g LNP, while this of BNNS/PVA/LNP film and BNNS/PVA/CNF film was only 30.66 MPa and 30.62 MPa, respectively	974:1160	Furthermore, the rigidity of composite film was strengthened by incorporating CNF and LNP, the tensile strength of the composite film showed the highest value of 35.0 MPa with 4 wt% CNF and 0.01 g LNP, while this of BNNS/PVA/LNP film and BNNS/PVA/CNF film was only 30.66 MPa and 30.62 MPa, respectively.
32344092	3	33	theme	4 wt	525:528	arg1	composites					546:555	The 4 wt% CNF-reinforced composites	521:555	The 4 wt% CNF-reinforced composites	521:555	The 4 wt% CNF-reinforced composites exhibited the higher through-plane thermal conductivity (up to 1.76 W/mK) than that without CNF (1.44 W/mK), revealing an increase of 22.2% at the same BNNS loading.
32344092	6	34	theme	wide	1269:1272	arg1	applications					1274:1285	wide applications	1269:1285	wide applications in thermal management of electronics	1269:1322	The green and flexible fabrication approaches will be helpful to build up eco-friendly BN composites with wide applications in thermal management of electronics.
32344092	0	35	theme	composite	17:25	arg1	film					27:30	BNNS/PVA bilayer composite film	0:30	BNNS/PVA bilayer composite film with multiple-improved properties by the synergistic actions of cellulose nanofibrils and lignin nanoparticles.	0:142	BNNS/PVA bilayer composite film with multiple-improved properties by the synergistic actions of cellulose nanofibrils and lignin nanoparticles.
32344092	1	36	theme	nanosheet	295:303	arg1	film					372:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	To avoid complex and toxic chemical cross-linking reaction, cellulose nanofibrils (CNF) was used to enhance the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film.
32344092	0	37	theme	nanoparticles	129:141	arg1	actions					85:91	the synergistic actions	69:91	the synergistic actions of cellulose nanofibrils and lignin nanoparticles	69:141	BNNS/PVA bilayer composite film with multiple-improved properties by the synergistic actions of cellulose nanofibrils and lignin nanoparticles.
32344092	1	38	theme	composite	362:370	arg1	film					372:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	To avoid complex and toxic chemical cross-linking reaction, cellulose nanofibrils (CNF) was used to enhance the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film.
32344092	3	39	theme	%	529:529	arg1	composites					546:555	The 4 wt% CNF-reinforced composites	521:555	The 4 wt% CNF-reinforced composites	521:555	The 4 wt% CNF-reinforced composites exhibited the higher through-plane thermal conductivity (up to 1.76 W/mK) than that without CNF (1.44 W/mK), revealing an increase of 22.2% at the same BNNS loading.
32344092	0	40	theme	multiple-improved	37:53	arg1	properties					55:64	multiple-improved properties	37:64	multiple-improved properties	37:64	BNNS/PVA bilayer composite film with multiple-improved properties by the synergistic actions of cellulose nanofibrils and lignin nanoparticles.
32344092	2	41	theme	film	515:518	arg1	stability					498:506	stability	498:506	stability	498:506	The results showed that CNF and LNP had synergistic actions on enhancing the tensile strength, thermal conductivity and stability of the film.
32344092	2	41	theme	film	515:518	arg1	strength					463:470	tensile strength	455:470	tensile strength	455:470	The results showed that CNF and LNP had synergistic actions on enhancing the tensile strength, thermal conductivity and stability of the film.
32344092	2	41	theme	film	515:518	arg1	conductivity					481:492	thermal conductivity	473:492	thermal conductivity	473:492	The results showed that CNF and LNP had synergistic actions on enhancing the tensile strength, thermal conductivity and stability of the film.
32344092	1	42	theme	film	372:375	arg1	performance					256:266	the performance	252:266	the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	252:375	To avoid complex and toxic chemical cross-linking reaction, cellulose nanofibrils (CNF) was used to enhance the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film.
32344092	3	43	theme	higher	571:576	arg1	conductivity					600:611	the higher through-plane thermal conductivity	567:611	the higher through-plane thermal conductivity (up to 1.76 W/mK)	567:629	The 4 wt% CNF-reinforced composites exhibited the higher through-plane thermal conductivity (up to 1.76 W/mK) than that without CNF (1.44 W/mK), revealing an increase of 22.2% at the same BNNS loading.
32344092	6	44	theme	thermal	1290:1296	arg1	management					1298:1307	thermal management	1290:1307	thermal management of electronics	1290:1322	The green and flexible fabrication approaches will be helpful to build up eco-friendly BN composites with wide applications in thermal management of electronics.
32344092	1	45	theme	BNNS	306:309	arg1	film					372:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	To avoid complex and toxic chemical cross-linking reaction, cellulose nanofibrils (CNF) was used to enhance the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film.
32344092	4	46	theme	composite	831:839	arg1	film					841:844	the composite film	827:844	the composite film without LNP	827:856	Meanwhile, the maximum decomposition rate (Rmax) was roughly 0.6%/oC, a decrease of 14.3% compared with the composite film without LNP.
32344092	3	47	theme	through-plane	578:590	arg1	conductivity					600:611	the higher through-plane thermal conductivity	567:611	the higher through-plane thermal conductivity (up to 1.76 W/mK)	567:629	The 4 wt% CNF-reinforced composites exhibited the higher through-plane thermal conductivity (up to 1.76 W/mK) than that without CNF (1.44 W/mK), revealing an increase of 22.2% at the same BNNS loading.
32344092	0	48	with	film	27:30	arg1	properties					55:64	multiple-improved properties	37:64	multiple-improved properties	37:64	BNNS/PVA bilayer composite film with multiple-improved properties by the synergistic actions of cellulose nanofibrils and lignin nanoparticles.
32344092	5	49	theme	4 wt	1035:1038	arg1	CNF					1041:1043	4 wt% CNF	1035:1043	4 wt% CNF	1035:1043	Furthermore, the rigidity of composite film was strengthened by incorporating CNF and LNP, the tensile strength of the composite film showed the highest value of 35.0 MPa with 4 wt% CNF and 0.01 g LNP, while this of BNNS/PVA/LNP film and BNNS/PVA/CNF film was only 30.66 MPa and 30.62 MPa, respectively.
32344092	6	50	theme	fabrication	1186:1196	arg1	approaches					1198:1207	The green and flexible fabrication approaches	1163:1207	The green and flexible fabrication approaches	1163:1207	The green and flexible fabrication approaches will be helpful to build up eco-friendly BN composites with wide applications in thermal management of electronics.
32344092	6	50	theme	fabrication	1186:1196	arg1	helpful					1217:1223	helpful	1217:1223	helpful	1217:1223	The green and flexible fabrication approaches will be helpful to build up eco-friendly BN composites with wide applications in thermal management of electronics.
32344092	3	51	theme	same	704:707	arg1	loading					714:720	the same BNNS loading	700:720	the same BNNS loading	700:720	The 4 wt% CNF-reinforced composites exhibited the higher through-plane thermal conductivity (up to 1.76 W/mK) than that without CNF (1.44 W/mK), revealing an increase of 22.2% at the same BNNS loading.
32344092	1	52	theme	/polyvinyl	311:320	arg1	film					372:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	To avoid complex and toxic chemical cross-linking reaction, cellulose nanofibrils (CNF) was used to enhance the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film.
32344092	6	53	theme	BN	1250:1251	arg1	composites					1253:1262	eco-friendly BN composites	1237:1262	eco-friendly BN composites with wide applications in thermal management of electronics	1237:1322	The green and flexible fabrication approaches will be helpful to build up eco-friendly BN composites with wide applications in thermal management of electronics.
32344092	4	54	dep	%	787:787	arg1	/oC					788:790	/oC	788:790	roughly 0.6%/oC	776:790	Meanwhile, the maximum decomposition rate (Rmax) was roughly 0.6%/oC, a decrease of 14.3% compared with the composite film without LNP.
32344092	1	55	theme	alcohol	322:328	arg1	film					372:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film	271:375	To avoid complex and toxic chemical cross-linking reaction, cellulose nanofibrils (CNF) was used to enhance the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film.
32344092	6	56	from	applications	1274:1285	arg1	management					1298:1307	thermal management	1290:1307	thermal management of electronics	1290:1322	The green and flexible fabrication approaches will be helpful to build up eco-friendly BN composites with wide applications in thermal management of electronics.
32344092	5	57	theme	composite	888:896	arg1	film					898:901	composite film	888:901	composite film	888:901	Furthermore, the rigidity of composite film was strengthened by incorporating CNF and LNP, the tensile strength of the composite film showed the highest value of 35.0 MPa with 4 wt% CNF and 0.01 g LNP, while this of BNNS/PVA/LNP film and BNNS/PVA/CNF film was only 30.66 MPa and 30.62 MPa, respectively.
32344092	3	58	theme	%	695:695	arg1	increase					679:686	an increase	676:686	an increase of 22.2% at the same BNNS loading	676:720	The 4 wt% CNF-reinforced composites exhibited the higher through-plane thermal conductivity (up to 1.76 W/mK) than that without CNF (1.44 W/mK), revealing an increase of 22.2% at the same BNNS loading.
32344092	6	59	with	composites	1253:1262	arg1	applications					1274:1285	wide applications	1269:1285	wide applications in thermal management of electronics	1269:1322	The green and flexible fabrication approaches will be helpful to build up eco-friendly BN composites with wide applications in thermal management of electronics.
32344092	4	60	theme	%	811:811	arg1	%					787:787	roughly 0.6%	776:787	roughly 0.6%/oC	776:790	Meanwhile, the maximum decomposition rate (Rmax) was roughly 0.6%/oC, a decrease of 14.3% compared with the composite film without LNP.
32344092	4	60	theme	%	811:811	arg1	decrease					795:802	a decrease	793:802	a decrease of 14.3%	793:811	Meanwhile, the maximum decomposition rate (Rmax) was roughly 0.6%/oC, a decrease of 14.3% compared with the composite film without LNP.
32344092	1	61	used	used	236:239	arg2	CNF					227:229	CNF	227:229	CNF	227:229	To avoid complex and toxic chemical cross-linking reaction, cellulose nanofibrils (CNF) was used to enhance the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film.
32344092	1	61	used	used	236:239	arg2	nanofibrils					214:224	cellulose nanofibrils	204:224	cellulose nanofibrils (CNF)	204:230	To avoid complex and toxic chemical cross-linking reaction, cellulose nanofibrils (CNF) was used to enhance the performance of hexagonal boron nitride nanosheet (BNNS)/polyvinyl alcohol (PVA)/lignin nanoparticle (LNP) composite film.
32344092	5	62	theme	film	898:901	arg1	rigidity					876:883	the rigidity	872:883	the rigidity of composite film	872:901	Furthermore, the rigidity of composite film was strengthened by incorporating CNF and LNP, the tensile strength of the composite film showed the highest value of 35.0 MPa with 4 wt% CNF and 0.01 g LNP, while this of BNNS/PVA/LNP film and BNNS/PVA/CNF film was only 30.66 MPa and 30.62 MPa, respectively.
32344092	5	63	theme	BNNS/PVA/CNF	1097:1108	arg1	film					1110:1113	BNNS/PVA/CNF film	1097:1113	BNNS/PVA/CNF film	1097:1113	Furthermore, the rigidity of composite film was strengthened by incorporating CNF and LNP, the tensile strength of the composite film showed the highest value of 35.0 MPa with 4 wt% CNF and 0.01 g LNP, while this of BNNS/PVA/LNP film and BNNS/PVA/CNF film was only 30.66 MPa and 30.62 MPa, respectively.
32344092	0	64	theme	synergistic	73:83	arg1	actions					85:91	the synergistic actions	69:91	the synergistic actions of cellulose nanofibrils and lignin nanoparticles	69:141	BNNS/PVA bilayer composite film with multiple-improved properties by the synergistic actions of cellulose nanofibrils and lignin nanoparticles.
32344092	5	65	theme	tensile	954:960	arg1	CNF					937:939	CNF	937:939	CNF	937:939	Furthermore, the rigidity of composite film was strengthened by incorporating CNF and LNP, the tensile strength of the composite film showed the highest value of 35.0 MPa with 4 wt% CNF and 0.01 g LNP, while this of BNNS/PVA/LNP film and BNNS/PVA/CNF film was only 30.66 MPa and 30.62 MPa, respectively.
32344092	5	65	theme	tensile	954:960	arg1	strength					962:969	the tensile strength	950:969	the tensile strength of the composite film showed the highest value of 35.0 MPa with 4 wt% CNF and 0.01 g LNP, while this of BNNS/PVA/LNP film and BNNS/PVA/CNF film was only 30.66 MPa and 30.62 MPa, respectively	950:1160	Furthermore, the rigidity of composite film was strengthened by incorporating CNF and LNP, the tensile strength of the composite film showed the highest value of 35.0 MPa with 4 wt% CNF and 0.01 g LNP, while this of BNNS/PVA/LNP film and BNNS/PVA/CNF film was only 30.66 MPa and 30.62 MPa, respectively.
32344092	3	66	theme	thermal	592:598	arg1	conductivity					600:611	the higher through-plane thermal conductivity	567:611	the higher through-plane thermal conductivity (up to 1.76 W/mK)	567:629	The 4 wt% CNF-reinforced composites exhibited the higher through-plane thermal conductivity (up to 1.76 W/mK) than that without CNF (1.44 W/mK), revealing an increase of 22.2% at the same BNNS loading.
32344092	5	67	theme	0.01 g	1049:1054	arg1	LNP					1056:1058	0.01 g LNP	1049:1058	0.01 g LNP	1049:1058	Furthermore, the rigidity of composite film was strengthened by incorporating CNF and LNP, the tensile strength of the composite film showed the highest value of 35.0 MPa with 4 wt% CNF and 0.01 g LNP, while this of BNNS/PVA/LNP film and BNNS/PVA/CNF film was only 30.66 MPa and 30.62 MPa, respectively.
32344092	4	68	theme	maximum	738:744	arg1	%					787:787	roughly 0.6%	776:787	roughly 0.6%/oC	776:790	Meanwhile, the maximum decomposition rate (Rmax) was roughly 0.6%/oC, a decrease of 14.3% compared with the composite film without LNP.
32344092	4	68	theme	maximum	738:744	arg1	Rmax					766:769	Rmax	766:769	Rmax	766:769	Meanwhile, the maximum decomposition rate (Rmax) was roughly 0.6%/oC, a decrease of 14.3% compared with the composite film without LNP.
32344092	4	68	theme	maximum	738:744	arg1	rate					760:763	the maximum decomposition rate	734:763	the maximum decomposition rate (Rmax)	734:770	Meanwhile, the maximum decomposition rate (Rmax) was roughly 0.6%/oC, a decrease of 14.3% compared with the composite film without LNP.
32344092	2	69	theme	synergistic	418:428	arg1	actions					430:436	synergistic actions	418:436	synergistic actions	418:436	The results showed that CNF and LNP had synergistic actions on enhancing the tensile strength, thermal conductivity and stability of the film.
32344092	5	70	theme	%	1039:1039	arg1	CNF					1041:1043	4 wt% CNF	1035:1043	4 wt% CNF	1035:1043	Furthermore, the rigidity of composite film was strengthened by incorporating CNF and LNP, the tensile strength of the composite film showed the highest value of 35.0 MPa with 4 wt% CNF and 0.01 g LNP, while this of BNNS/PVA/LNP film and BNNS/PVA/CNF film was only 30.66 MPa and 30.62 MPa, respectively.
32776549	5	0	from	reduction	982:990	arg1	starch					1005:1010	resistant starch	995:1010	resistant starch of 54.2%	995:1019	Meanwhile, extrusion showed a reduction in resistant starch of 54.2%.
32776549	11	1	theme	treated	1680:1686	arg1	extracts					1712:1719	Thermally treated black bean carbohydrate extracts	1670:1719	Thermally treated black bean carbohydrate extracts	1670:1719	PRACTICAL APPLICATION: Thermally treated black bean carbohydrate extracts are rich in starch, fiber, and protein.
32776549	5	2	theme	%	1019:1019	arg1	starch					1005:1010	resistant starch	995:1010	resistant starch of 54.2%	995:1019	Meanwhile, extrusion showed a reduction in resistant starch of 54.2%.
32776549	4	3	theme	starch	877:882	arg1	reduction					854:862	a reduction	852:862	a reduction of resistant starch	852:882	Autoclaving treatments at 100 °C for 30 min and 121 °C for 15 min 2× showed a reduction of resistant starch by 14.4% and 26.6%, respectively, compared to dehulled raw bean CE.
32776549	2	4	theme	black	549:553	arg1	beans					555:559	autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans	486:559	autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans	486:559	The purpose of this study was to evaluate the composition, morphology, thermal, functional, and physicochemical properties of carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans.
32776549	11	5	theme	carbohydrate	1699:1710	arg1	extracts					1712:1719	Thermally treated black bean carbohydrate extracts	1670:1719	Thermally treated black bean carbohydrate extracts	1670:1719	PRACTICAL APPLICATION: Thermally treated black bean carbohydrate extracts are rich in starch, fiber, and protein.
32776549	3	6	theme	autoclaved	621:630	arg1	beans					632:636	autoclaved beans	621:636	autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×	621:682	After evaluation of the functional properties, the CE from autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×, and extruded beans at 120 °C and 10 rpm, were selected to continue the remaining analysis.
32776549	6	7	theme	dietary	1078:1084	arg1	content					1092:1098	the dietary fiber content	1074:1098	the dietary fiber content	1074:1098	Autoclaving and extrusion treatments also decreased the dietary fiber content.
32776549	10	8	theme	food	1562:1565	arg1	products					1567:1574	food products	1562:1574	food products as a thickening agent for puddings, sauces, creams, or dairy products	1562:1644	The CE from autoclaved and extruded beans contain retrograded and pregelatinized starch, which could be incorporated in food products as a thickening agent for puddings, sauces, creams, or dairy products.
32776549	3	9	from	beans	632:636	arg1	CE					613:614	the CE	609:614	the CE from autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×	609:682	After evaluation of the functional properties, the CE from autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×, and extruded beans at 120 °C and 10 rpm, were selected to continue the remaining analysis.
32776549	3	9	from	beans	632:636	arg1	beans					698:702	extruded beans	689:702	extruded beans at 120 °C and 10 rpm	689:723	After evaluation of the functional properties, the CE from autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×, and extruded beans at 120 °C and 10 rpm, were selected to continue the remaining analysis.
32776549	4	10	from	°C	806:807	arg1	treatments					788:797	Autoclaving treatments	776:797	Autoclaving treatments at 100 °C for 30 min and 121 °C for 15 min 2×	776:843	Autoclaving treatments at 100 °C for 30 min and 121 °C for 15 min 2× showed a reduction of resistant starch by 14.4% and 26.6%, respectively, compared to dehulled raw bean CE.
32776549	10	11	theme	thickening	1581:1590	arg1	agent					1592:1596	a thickening agent	1579:1596	a thickening agent for puddings, sauces, creams, or dairy products	1579:1644	The CE from autoclaved and extruded beans contain retrograded and pregelatinized starch, which could be incorporated in food products as a thickening agent for puddings, sauces, creams, or dairy products.
32776549	1	12	theme	high	185:188	arg1	content					190:196	a high content	183:196	a high content of amylose, which is associated with the production of retrograded and pregelatinized starch through thermal treatments	183:316	Common beans (Phaseolus vulgaris L.) are rich in starch with a high content of amylose, which is associated with the production of retrograded and pregelatinized starch through thermal treatments.
32776549	4	13	theme	raw	939:941	arg1	CE					948:949	dehulled raw bean CE	930:949	dehulled raw bean CE	930:949	Autoclaving treatments at 100 °C for 30 min and 121 °C for 15 min 2× showed a reduction of resistant starch by 14.4% and 26.6%, respectively, compared to dehulled raw bean CE.
32776549	9	14	theme	conclusion	1301:1310	arg1	temperatures					1312:1323	The onset, peak, and conclusion temperatures	1280:1323	The onset, peak, and conclusion temperatures	1280:1323	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	9	14	theme	conclusion	1301:1310	arg1	temperature					1337:1347	transition temperature range	1326:1353	transition temperature range	1326:1353	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	9	14	theme	conclusion	1301:1310	arg1	lower					1409:1413	lower	1409:1413	lower	1409:1413	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	9	14	theme	conclusion	1301:1310	arg1	enthalpy					1360:1367	enthalpy	1360:1367	enthalpy of autoclaved and extruded bean CE	1360:1402	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	11	15	from	starch	1733:1738	arg1	rich					1725:1728	rich	1725:1728	rich	1725:1728	PRACTICAL APPLICATION: Thermally treated black bean carbohydrate extracts are rich in starch, fiber, and protein.
32776549	5	16	theme	resistant	995:1003	arg1	starch					1005:1010	resistant starch	995:1010	resistant starch of 54.2%	995:1019	Meanwhile, extrusion showed a reduction in resistant starch of 54.2%.
32776549	7	17	theme	bean	1207:1210	arg1	CE					1212:1213	dehulled raw bean CE	1194:1213	dehulled raw bean CE	1194:1213	Extrusion reduced almost entirely the content of α-galactooligosaccharides, in comparison to dehulled raw bean CE.
32776549	2	18	theme	extruded	518:525	arg1	beans					555:559	autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans	486:559	autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans	486:559	The purpose of this study was to evaluate the composition, morphology, thermal, functional, and physicochemical properties of carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans.
32776549	3	19	theme	121	663:665	arg1	°C					667:668	121 °C	663:668	121 °C	663:668	After evaluation of the functional properties, the CE from autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×, and extruded beans at 120 °C and 10 rpm, were selected to continue the remaining analysis.
32776549	4	20	theme	Autoclaving	776:786	arg1	treatments					788:797	Autoclaving treatments	776:797	Autoclaving treatments at 100 °C for 30 min and 121 °C for 15 min 2×	776:843	Autoclaving treatments at 100 °C for 30 min and 121 °C for 15 min 2× showed a reduction of resistant starch by 14.4% and 26.6%, respectively, compared to dehulled raw bean CE.
32776549	7	21	theme	dehulled	1194:1201	arg1	CE					1212:1213	dehulled raw bean CE	1194:1213	dehulled raw bean CE	1194:1213	Extrusion reduced almost entirely the content of α-galactooligosaccharides, in comparison to dehulled raw bean CE.
32776549	11	22	from	fiber	1741:1745	arg1	rich					1725:1728	rich	1725:1728	rich	1725:1728	PRACTICAL APPLICATION: Thermally treated black bean carbohydrate extracts are rich in starch, fiber, and protein.
32776549	6	23	dep	Autoclaving	1022:1032	arg1	treatments					1048:1057	treatments	1048:1057	treatments	1048:1057	Autoclaving and extrusion treatments also decreased the dietary fiber content.
32776549	10	24	theme	dairy	1631:1635	arg1	products					1637:1644	dairy products	1631:1644	dairy products	1631:1644	The CE from autoclaved and extruded beans contain retrograded and pregelatinized starch, which could be incorporated in food products as a thickening agent for puddings, sauces, creams, or dairy products.
32776549	1	25	from	starch	171:176	arg1	rich					163:166	rich	163:166	rich	163:166	Common beans (Phaseolus vulgaris L.) are rich in starch with a high content of amylose, which is associated with the production of retrograded and pregelatinized starch through thermal treatments.
32776549	1	26	theme	Phaseolus	136:144	arg1	L.					155:156	L.	155:156	L.	155:156	Common beans (Phaseolus vulgaris L.) are rich in starch with a high content of amylose, which is associated with the production of retrograded and pregelatinized starch through thermal treatments.
32776549	1	26	theme	Phaseolus	136:144	arg1	vulgaris					146:153	Phaseolus vulgaris	136:153	Phaseolus vulgaris L.	136:156	Common beans (Phaseolus vulgaris L.) are rich in starch with a high content of amylose, which is associated with the production of retrograded and pregelatinized starch through thermal treatments.
32776549	0	27	theme	chemical	64:71	arg1	composition					73:83	chemical composition	64:83	chemical composition	64:83	Autoclaving and extrusion improve the functional properties and chemical composition of black bean carbohydrate extracts.
32776549	1	28	from	rich	163:166	arg1	starch					171:176	starch	171:176	starch with a high content of amylose, which is associated with the production of retrograded and pregelatinized starch through thermal treatments	171:316	Common beans (Phaseolus vulgaris L.) are rich in starch with a high content of amylose, which is associated with the production of retrograded and pregelatinized starch through thermal treatments.
32776549	11	29	from	rich	1725:1728	arg1	protein					1752:1758	protein	1752:1758	protein	1752:1758	PRACTICAL APPLICATION: Thermally treated black bean carbohydrate extracts are rich in starch, fiber, and protein.
32776549	11	29	from	rich	1725:1728	arg1	starch					1733:1738	starch	1733:1738	starch	1733:1738	PRACTICAL APPLICATION: Thermally treated black bean carbohydrate extracts are rich in starch, fiber, and protein.
32776549	11	29	from	rich	1725:1728	arg1	fiber					1741:1745	fiber	1741:1745	fiber	1741:1745	PRACTICAL APPLICATION: Thermally treated black bean carbohydrate extracts are rich in starch, fiber, and protein.
32776549	11	30	from	protein	1752:1758	arg1	rich					1725:1728	rich	1725:1728	rich	1725:1728	PRACTICAL APPLICATION: Thermally treated black bean carbohydrate extracts are rich in starch, fiber, and protein.
32776549	0	31	theme	bean	94:97	arg1	extracts					112:119	black bean carbohydrate extracts	88:119	black bean carbohydrate extracts	88:119	Autoclaving and extrusion improve the functional properties and chemical composition of black bean carbohydrate extracts.
32776549	9	32	theme	autoclaved	1372:1381	arg1	CE					1401:1402	autoclaved and extruded bean CE	1372:1402	autoclaved and extruded bean CE	1372:1402	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	2	33	theme	autoclaved	486:495	arg1	beans					555:559	autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans	486:559	autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans	486:559	The purpose of this study was to evaluate the composition, morphology, thermal, functional, and physicochemical properties of carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans.
32776549	3	34	from	°C	645:646	arg1	beans					632:636	autoclaved beans	621:636	autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×	621:682	After evaluation of the functional properties, the CE from autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×, and extruded beans at 120 °C and 10 rpm, were selected to continue the remaining analysis.
32776549	2	35	theme	thermal	390:396	arg1	composition					365:375	the composition	361:375	the composition	361:375	The purpose of this study was to evaluate the composition, morphology, thermal, functional, and physicochemical properties of carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans.
32776549	10	36	theme	extruded	1469:1476	arg1	beans					1478:1482	autoclaved and extruded beans	1454:1482	autoclaved and extruded beans	1454:1482	The CE from autoclaved and extruded beans contain retrograded and pregelatinized starch, which could be incorporated in food products as a thickening agent for puddings, sauces, creams, or dairy products.
32776549	0	37	theme	extracts	112:119	arg1	properties					49:58	the functional properties	34:58	the functional properties	34:58	Autoclaving and extrusion improve the functional properties and chemical composition of black bean carbohydrate extracts.
32776549	0	37	theme	extracts	112:119	arg1	composition					73:83	chemical composition	64:83	chemical composition	64:83	Autoclaving and extrusion improve the functional properties and chemical composition of black bean carbohydrate extracts.
32776549	9	38	theme	extruded	1387:1394	arg1	CE					1401:1402	autoclaved and extruded bean CE	1372:1402	autoclaved and extruded bean CE	1372:1402	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	3	39	theme	remaining	756:764	arg1	analysis					766:773	the remaining analysis	752:773	the remaining analysis	752:773	After evaluation of the functional properties, the CE from autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×, and extruded beans at 120 °C and 10 rpm, were selected to continue the remaining analysis.
32776549	2	40	theme	physicochemical	415:429	arg1	properties					431:440	the composition, morphology, thermal, functional, and physicochemical properties	361:440	the composition, morphology, thermal, functional, and physicochemical properties of carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans	361:559	The purpose of this study was to evaluate the composition, morphology, thermal, functional, and physicochemical properties of carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans.
32776549	10	41	theme	autoclaved	1454:1463	arg1	beans					1478:1482	autoclaved and extruded beans	1454:1482	autoclaved and extruded beans	1454:1482	The CE from autoclaved and extruded beans contain retrograded and pregelatinized starch, which could be incorporated in food products as a thickening agent for puddings, sauces, creams, or dairy products.
32776549	9	42	theme	CE	1401:1402	arg1	temperatures					1312:1323	The onset, peak, and conclusion temperatures	1280:1323	The onset, peak, and conclusion temperatures	1280:1323	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	9	42	theme	CE	1401:1402	arg1	temperature					1337:1347	transition temperature range	1326:1353	transition temperature range	1326:1353	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	9	42	theme	CE	1401:1402	arg1	enthalpy					1360:1367	enthalpy	1360:1367	enthalpy of autoclaved and extruded bean CE	1360:1402	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	7	43	theme	α-galactooligosaccharides	1150:1174	arg1	content					1139:1145	the content	1135:1145	the content of α-galactooligosaccharides	1135:1174	Extrusion reduced almost entirely the content of α-galactooligosaccharides, in comparison to dehulled raw bean CE.
32776549	2	44	theme	extracts	458:465	arg1	properties					431:440	the composition, morphology, thermal, functional, and physicochemical properties	361:440	the composition, morphology, thermal, functional, and physicochemical properties of carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans	361:559	The purpose of this study was to evaluate the composition, morphology, thermal, functional, and physicochemical properties of carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans.
32776549	1	45	theme	starch	284:289	arg1	production					239:248	the production	235:248	the production of retrograded and pregelatinized starch through thermal treatments	235:316	Common beans (Phaseolus vulgaris L.) are rich in starch with a high content of amylose, which is associated with the production of retrograded and pregelatinized starch through thermal treatments.
32776549	9	46	theme	dehulled	1420:1427	arg1	CE					1438:1439	dehulled raw bean CE	1420:1439	dehulled raw bean CE	1420:1439	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	12	47	theme	thickening	1937:1946	arg1	agent					1948:1952	a thickening agent	1935:1952	a thickening agent	1935:1952	Because these extracts are already cooked, they can be added to products that do not require a thermal process such as puddings, sauces, creams, or dairy products, acting as a thickening agent.
32776549	1	48	theme	thermal	299:305	arg1	treatments					307:316	thermal treatments	299:316	thermal treatments	299:316	Common beans (Phaseolus vulgaris L.) are rich in starch with a high content of amylose, which is associated with the production of retrograded and pregelatinized starch through thermal treatments.
32776549	9	49	theme	bean	1433:1436	arg1	CE					1438:1439	dehulled raw bean CE	1420:1439	dehulled raw bean CE	1420:1439	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	4	50	theme	121	824:826	arg1	°C					828:829	121 °C	824:829	121 °C	824:829	Autoclaving treatments at 100 °C for 30 min and 121 °C for 15 min 2× showed a reduction of resistant starch by 14.4% and 26.6%, respectively, compared to dehulled raw bean CE.
32776549	3	51	theme	properties	597:606	arg1	evaluation					568:577	evaluation	568:577	evaluation of the functional properties	568:606	After evaluation of the functional properties, the CE from autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×, and extruded beans at 120 °C and 10 rpm, were selected to continue the remaining analysis.
32776549	12	52	theme	dairy	1909:1913	arg1	products					1915:1922	dairy products	1909:1922	dairy products	1909:1922	Because these extracts are already cooked, they can be added to products that do not require a thermal process such as puddings, sauces, creams, or dairy products, acting as a thickening agent.
32776549	1	53	theme	amylose	201:207	arg1	content					190:196	a high content	183:196	a high content of amylose, which is associated with the production of retrograded and pregelatinized starch through thermal treatments	183:316	Common beans (Phaseolus vulgaris L.) are rich in starch with a high content of amylose, which is associated with the production of retrograded and pregelatinized starch through thermal treatments.
32776549	11	54	theme	black	1688:1692	arg1	extracts					1712:1719	Thermally treated black bean carbohydrate extracts	1670:1719	Thermally treated black bean carbohydrate extracts	1670:1719	PRACTICAL APPLICATION: Thermally treated black bean carbohydrate extracts are rich in starch, fiber, and protein.
32776549	2	55	theme	study	339:343	arg1	purpose					323:329	The purpose	319:329	The purpose of this study	319:343	The purpose of this study was to evaluate the composition, morphology, thermal, functional, and physicochemical properties of carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans.
32776549	6	56	theme	fiber	1086:1090	arg1	content					1092:1098	the dietary fiber content	1074:1098	the dietary fiber content	1074:1098	Autoclaving and extrusion treatments also decreased the dietary fiber content.
32776549	8	57	from	differences	1235:1245	arg1	color					1250:1254	color	1250:1254	color	1250:1254	The results showed differences in color and granule morphology.
32776549	8	57	from	differences	1235:1245	arg1	morphology					1268:1277	granule morphology	1260:1277	granule morphology	1260:1277	The results showed differences in color and granule morphology.
32776549	10	58	contain	contain	1484:1490	arg2	starch					1523:1528	retrograded and pregelatinized starch	1492:1528	retrograded and pregelatinized starch	1492:1528	The CE from autoclaved and extruded beans contain retrograded and pregelatinized starch, which could be incorporated in food products as a thickening agent for puddings, sauces, creams, or dairy products.
32776549	10	58	contain	contain	1484:1490	arg1	CE					1446:1447	The CE	1442:1447	The CE from autoclaved and extruded beans	1442:1482	The CE from autoclaved and extruded beans contain retrograded and pregelatinized starch, which could be incorporated in food products as a thickening agent for puddings, sauces, creams, or dairy products.
32776549	9	59	theme	onset	1284:1288	arg1	temperatures					1312:1323	The onset, peak, and conclusion temperatures	1280:1323	The onset, peak, and conclusion temperatures	1280:1323	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	9	59	theme	onset	1284:1288	arg1	temperature					1337:1347	transition temperature range	1326:1353	transition temperature range	1326:1353	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	9	59	theme	onset	1284:1288	arg1	lower					1409:1413	lower	1409:1413	lower	1409:1413	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	9	59	theme	onset	1284:1288	arg1	enthalpy					1360:1367	enthalpy	1360:1367	enthalpy of autoclaved and extruded bean CE	1360:1402	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	3	60	from	10 rpm	718:723	arg1	CE					613:614	the CE	609:614	the CE from autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×	609:682	After evaluation of the functional properties, the CE from autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×, and extruded beans at 120 °C and 10 rpm, were selected to continue the remaining analysis.
32776549	3	60	from	10 rpm	718:723	arg1	beans					698:702	extruded beans	689:702	extruded beans at 120 °C and 10 rpm	689:723	After evaluation of the functional properties, the CE from autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×, and extruded beans at 120 °C and 10 rpm, were selected to continue the remaining analysis.
32776549	4	61	theme	dehulled	930:937	arg1	CE					948:949	dehulled raw bean CE	930:949	dehulled raw bean CE	930:949	Autoclaving treatments at 100 °C for 30 min and 121 °C for 15 min 2× showed a reduction of resistant starch by 14.4% and 26.6%, respectively, compared to dehulled raw bean CE.
32776549	9	62	theme	peak	1291:1294	arg1	temperatures					1312:1323	The onset, peak, and conclusion temperatures	1280:1323	The onset, peak, and conclusion temperatures	1280:1323	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	9	62	theme	peak	1291:1294	arg1	temperature					1337:1347	transition temperature range	1326:1353	transition temperature range	1326:1353	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	9	62	theme	peak	1291:1294	arg1	lower					1409:1413	lower	1409:1413	lower	1409:1413	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	9	62	theme	peak	1291:1294	arg1	enthalpy					1360:1367	enthalpy	1360:1367	enthalpy of autoclaved and extruded bean CE	1360:1402	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	4	63	theme	bean	943:946	arg1	CE					948:949	dehulled raw bean CE	930:949	dehulled raw bean CE	930:949	Autoclaving treatments at 100 °C for 30 min and 121 °C for 15 min 2× showed a reduction of resistant starch by 14.4% and 26.6%, respectively, compared to dehulled raw bean CE.
32776549	3	64	theme	15 min	674:679	arg1	2×					681:682	15 min 2×	674:682	15 min 2×	674:682	After evaluation of the functional properties, the CE from autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×, and extruded beans at 120 °C and 10 rpm, were selected to continue the remaining analysis.
32776549	1	65	theme	Common	122:127	arg1	beans					129:133	Common beans	122:133	Common beans (Phaseolus vulgaris L.)	122:157	Common beans (Phaseolus vulgaris L.) are rich in starch with a high content of amylose, which is associated with the production of retrograded and pregelatinized starch through thermal treatments.
32776549	9	66	theme	transition	1326:1335	arg1	temperatures					1312:1323	The onset, peak, and conclusion temperatures	1280:1323	The onset, peak, and conclusion temperatures	1280:1323	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	9	66	theme	transition	1326:1335	arg1	temperature					1337:1347	transition temperature range	1326:1353	transition temperature range	1326:1353	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	7	67	theme	raw	1203:1205	arg1	CE					1212:1213	dehulled raw bean CE	1194:1213	dehulled raw bean CE	1194:1213	Extrusion reduced almost entirely the content of α-galactooligosaccharides, in comparison to dehulled raw bean CE.
32776549	12	68	theme	thermal	1856:1862	arg1	process					1864:1870	a thermal process	1854:1870	a thermal process such as puddings, sauces, creams, or dairy products	1854:1922	Because these extracts are already cooked, they can be added to products that do not require a thermal process such as puddings, sauces, creams, or dairy products, acting as a thickening agent.
32776549	3	69	theme	extruded	689:696	arg1	beans					698:702	extruded beans	689:702	extruded beans at 120 °C and 10 rpm	689:723	After evaluation of the functional properties, the CE from autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×, and extruded beans at 120 °C and 10 rpm, were selected to continue the remaining analysis.
32776549	0	70	theme	black	88:92	arg1	extracts					112:119	black bean carbohydrate extracts	88:119	black bean carbohydrate extracts	88:119	Autoclaving and extrusion improve the functional properties and chemical composition of black bean carbohydrate extracts.
32776549	4	71	theme	15 min	835:840	arg1	2×					842:843	15 min 2×	835:843	15 min 2×	835:843	Autoclaving treatments at 100 °C for 30 min and 121 °C for 15 min 2× showed a reduction of resistant starch by 14.4% and 26.6%, respectively, compared to dehulled raw bean CE.
32776549	0	72	theme	carbohydrate	99:110	arg1	extracts					112:119	black bean carbohydrate extracts	88:119	black bean carbohydrate extracts	88:119	Autoclaving and extrusion improve the functional properties and chemical composition of black bean carbohydrate extracts.
32776549	9	73	dep	temperature	1337:1347	arg1	range					1349:1353	range	1349:1353	transition temperature range	1326:1353	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	11	74	theme	PRACTICAL	1647:1655	arg1	APPLICATION					1657:1667	PRACTICAL APPLICATION	1647:1667	PRACTICAL APPLICATION: Thermally treated black bean carbohydrate extracts are rich in starch, fiber, and protein.	1647:1759	PRACTICAL APPLICATION: Thermally treated black bean carbohydrate extracts are rich in starch, fiber, and protein.
32776549	11	75	dep	APPLICATION	1657:1667	arg1	rich					1725:1728	rich	1725:1728	rich	1725:1728	PRACTICAL APPLICATION: Thermally treated black bean carbohydrate extracts are rich in starch, fiber, and protein.
32776549	1	76	dep	beans	129:133	arg1	L.					155:156	L.	155:156	L.	155:156	Common beans (Phaseolus vulgaris L.) are rich in starch with a high content of amylose, which is associated with the production of retrograded and pregelatinized starch through thermal treatments.
32776549	1	76	dep	beans	129:133	arg1	vulgaris					146:153	Phaseolus vulgaris	136:153	Phaseolus vulgaris L.	136:156	Common beans (Phaseolus vulgaris L.) are rich in starch with a high content of amylose, which is associated with the production of retrograded and pregelatinized starch through thermal treatments.
32776549	2	77	theme	composition	365:375	arg1	properties					431:440	the composition, morphology, thermal, functional, and physicochemical properties	361:440	the composition, morphology, thermal, functional, and physicochemical properties of carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans	361:559	The purpose of this study was to evaluate the composition, morphology, thermal, functional, and physicochemical properties of carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans.
32776549	9	78	theme	bean	1396:1399	arg1	CE					1401:1402	autoclaved and extruded bean CE	1372:1402	autoclaved and extruded bean CE	1372:1402	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	4	79	theme	resistant	867:875	arg1	starch					877:882	resistant starch	867:882	resistant starch	867:882	Autoclaving treatments at 100 °C for 30 min and 121 °C for 15 min 2× showed a reduction of resistant starch by 14.4% and 26.6%, respectively, compared to dehulled raw bean CE.
32776549	1	80	theme	retrograded	253:263	arg1	starch					284:289	retrograded and pregelatinized starch	253:289	retrograded and pregelatinized starch	253:289	Common beans (Phaseolus vulgaris L.) are rich in starch with a high content of amylose, which is associated with the production of retrograded and pregelatinized starch through thermal treatments.
32776549	2	81	dep	autoclaved	486:495	arg1	°C					510:511	100 and 121 °C	498:511	100 and 121 °C	498:511	The purpose of this study was to evaluate the composition, morphology, thermal, functional, and physicochemical properties of carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans.
32776549	2	82	theme	functional	399:408	arg1	composition					365:375	the composition	361:375	the composition	361:375	The purpose of this study was to evaluate the composition, morphology, thermal, functional, and physicochemical properties of carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans.
32776549	10	83	theme	retrograded	1492:1502	arg1	starch					1523:1528	retrograded and pregelatinized starch	1492:1528	retrograded and pregelatinized starch	1492:1528	The CE from autoclaved and extruded beans contain retrograded and pregelatinized starch, which could be incorporated in food products as a thickening agent for puddings, sauces, creams, or dairy products.
32776549	0	84	theme	functional	38:47	arg1	properties					49:58	the functional properties	34:58	the functional properties	34:58	Autoclaving and extrusion improve the functional properties and chemical composition of black bean carbohydrate extracts.
32776549	1	85	theme	pregelatinized	269:282	arg1	starch					284:289	retrograded and pregelatinized starch	253:289	retrograded and pregelatinized starch	253:289	Common beans (Phaseolus vulgaris L.) are rich in starch with a high content of amylose, which is associated with the production of retrograded and pregelatinized starch through thermal treatments.
32776549	2	86	theme	carbohydrate	445:456	arg1	CE					468:469	CE	468:469	CE	468:469	The purpose of this study was to evaluate the composition, morphology, thermal, functional, and physicochemical properties of carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans.
32776549	2	86	theme	carbohydrate	445:456	arg1	extracts					458:465	carbohydrate extracts	445:465	carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans	445:559	The purpose of this study was to evaluate the composition, morphology, thermal, functional, and physicochemical properties of carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans.
32776549	3	87	from	°C	711:712	arg1	CE					613:614	the CE	609:614	the CE from autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×	609:682	After evaluation of the functional properties, the CE from autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×, and extruded beans at 120 °C and 10 rpm, were selected to continue the remaining analysis.
32776549	3	87	from	°C	711:712	arg1	beans					698:702	extruded beans	689:702	extruded beans at 120 °C and 10 rpm	689:723	After evaluation of the functional properties, the CE from autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×, and extruded beans at 120 °C and 10 rpm, were selected to continue the remaining analysis.
32776549	2	88	theme	morphology	378:387	arg1	properties					431:440	the composition, morphology, thermal, functional, and physicochemical properties	361:440	the composition, morphology, thermal, functional, and physicochemical properties of carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans	361:559	The purpose of this study was to evaluate the composition, morphology, thermal, functional, and physicochemical properties of carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans.
32776549	1	89	with	starch	171:176	arg1	content					190:196	a high content	183:196	a high content of amylose, which is associated with the production of retrograded and pregelatinized starch through thermal treatments	183:316	Common beans (Phaseolus vulgaris L.) are rich in starch with a high content of amylose, which is associated with the production of retrograded and pregelatinized starch through thermal treatments.
32776549	9	90	theme	raw	1429:1431	arg1	CE					1438:1439	dehulled raw bean CE	1420:1439	dehulled raw bean CE	1420:1439	The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE.
32776549	3	91	theme	functional	586:595	arg1	properties					597:606	the functional properties	582:606	the functional properties	582:606	After evaluation of the functional properties, the CE from autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×, and extruded beans at 120 °C and 10 rpm, were selected to continue the remaining analysis.
32776549	10	92	theme	pregelatinized	1508:1521	arg1	starch					1523:1528	retrograded and pregelatinized starch	1492:1528	retrograded and pregelatinized starch	1492:1528	The CE from autoclaved and extruded beans contain retrograded and pregelatinized starch, which could be incorporated in food products as a thickening agent for puddings, sauces, creams, or dairy products.
32776549	2	93	dep	extruded	518:525	arg1	°C					545:546	90, 105, and 120 °C	528:546	90, 105, and 120 °C	528:546	The purpose of this study was to evaluate the composition, morphology, thermal, functional, and physicochemical properties of carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans.
32776549	10	94	from	beans	1478:1482	arg1	CE					1446:1447	The CE	1442:1447	The CE from autoclaved and extruded beans	1442:1482	The CE from autoclaved and extruded beans contain retrograded and pregelatinized starch, which could be incorporated in food products as a thickening agent for puddings, sauces, creams, or dairy products.
32776549	8	95	theme	granule	1260:1266	arg1	morphology					1268:1277	granule morphology	1260:1277	granule morphology	1260:1277	The results showed differences in color and granule morphology.
32776549	11	96	theme	bean	1694:1697	arg1	extracts					1712:1719	Thermally treated black bean carbohydrate extracts	1670:1719	Thermally treated black bean carbohydrate extracts	1670:1719	PRACTICAL APPLICATION: Thermally treated black bean carbohydrate extracts are rich in starch, fiber, and protein.
34323711	0	0	theme	potential	95:103	arg1	application					105:115	potential application	95:115	potential application in hydrophobic organic contaminant removal	95:158	Fabrication of chitin nanofiber-PDMS composite aerogels from Pickering emulsion templates with potential application in hydrophobic organic contaminant removal.
34323711	10	1	theme	materials	1528:1536	arg1	fabrication					1491:1501	the fabrication	1487:1501	the fabrication of composite hydrophobic materials from natural biopolymers	1487:1561	This work reveals the potential of Pickering emulsions in the fabrication of composite hydrophobic materials from natural biopolymers with promising application in HOCs related water treatment.
34323711	10	2	theme	related	1598:1604	arg1	treatment					1612:1620	HOCs related water treatment	1593:1620	HOCs related water treatment	1593:1620	This work reveals the potential of Pickering emulsions in the fabrication of composite hydrophobic materials from natural biopolymers with promising application in HOCs related water treatment.
34323711	1	3	theme	aroused	183:189	arg1	attention					202:210	aroused increasing attention	183:210	aroused increasing attention	183:210	Natural polymers have aroused increasing attention in water treatment but their application in removing hydrophobic organic contaminants (HOCs) was limited due to their hydrophilicity.
34323711	6	4	theme	PDMS	1061:1064	arg1	contents					1066:1073	PDMS contents	1061:1073	PDMS contents	1061:1073	The CCl4 adsorption capacity was 521-2820 wt%, depending on PDMS contents.
34323711	2	5	theme	HOCs	589:592	arg1	removal					594:600	HOCs removal	589:600	HOCs removal	589:600	Herein, hydrophobic aerogels were successfully fabricated from Pickering emulsions stabilized by chitin nanofibers (ChNF) with polydimethylsiloxane (PDMS) as dispersed phase and glutaraldehyde as a crosslinking agent, and their performance in HOCs removal were evaluated.
34323711	2	6	with	nanofibers	450:459	arg1	PDMS					495:498	PDMS	495:498	PDMS	495:498	Herein, hydrophobic aerogels were successfully fabricated from Pickering emulsions stabilized by chitin nanofibers (ChNF) with polydimethylsiloxane (PDMS) as dispersed phase and glutaraldehyde as a crosslinking agent, and their performance in HOCs removal were evaluated.
34323711	2	6	with	nanofibers	450:459	arg1	polydimethylsiloxane					473:492	polydimethylsiloxane	473:492	polydimethylsiloxane (PDMS)	473:499	Herein, hydrophobic aerogels were successfully fabricated from Pickering emulsions stabilized by chitin nanofibers (ChNF) with polydimethylsiloxane (PDMS) as dispersed phase and glutaraldehyde as a crosslinking agent, and their performance in HOCs removal were evaluated.
34323711	10	7	theme	hydrophobic	1516:1526	arg1	materials					1528:1536	composite hydrophobic materials	1506:1536	composite hydrophobic materials	1506:1536	This work reveals the potential of Pickering emulsions in the fabrication of composite hydrophobic materials from natural biopolymers with promising application in HOCs related water treatment.
34323711	10	8	theme	Pickering	1464:1472	arg1	emulsions					1474:1482	Pickering emulsions	1464:1482	Pickering emulsions	1464:1482	This work reveals the potential of Pickering emulsions in the fabrication of composite hydrophobic materials from natural biopolymers with promising application in HOCs related water treatment.
34323711	1	9	theme	increasing	191:200	arg1	attention					202:210	aroused increasing attention	183:210	aroused increasing attention	183:210	Natural polymers have aroused increasing attention in water treatment but their application in removing hydrophobic organic contaminants (HOCs) was limited due to their hydrophilicity.
34323711	8	10	theme	adsorption	1243:1252	arg1	capacity					1254:1261	The adsorption capacity	1239:1261	The adsorption capacity	1239:1261	The adsorption capacity remained above 85% for 24 cycles.
34323711	3	11	theme	aerogel	730:736	arg1	templates					738:746	aerogel templates	730:746	aerogel templates	730:746	The Pickering emulsions with PDMS ratios of 2.5-20% v/v showed high stability, demonstrating great potential as aerogel templates.
34323711	3	11	theme	aerogel	730:736	arg1	potential					717:725	great potential	711:725	great potential	711:725	The Pickering emulsions with PDMS ratios of 2.5-20% v/v showed high stability, demonstrating great potential as aerogel templates.
34323711	10	12	theme	composite	1506:1514	arg1	materials					1528:1536	composite hydrophobic materials	1506:1536	composite hydrophobic materials	1506:1536	This work reveals the potential of Pickering emulsions in the fabrication of composite hydrophobic materials from natural biopolymers with promising application in HOCs related water treatment.
34323711	3	13	with	emulsions	632:640	arg1	ratios					652:657	PDMS ratios	647:657	PDMS ratios of 2.5-20% v/v	647:672	The Pickering emulsions with PDMS ratios of 2.5-20% v/v showed high stability, demonstrating great potential as aerogel templates.
34323711	9	14	theme	maximum	1371:1377	arg1	capacity					1390:1397	a maximum adsorption capacity	1369:1397	a maximum adsorption capacity of 1.34 mg/g for 10 mg/L TCE	1369:1426	Moreover, the aerogels could also remove dissolved HOCs from water with a maximum adsorption capacity of 1.34 mg/g for 10 mg/L TCE.
34323711	7	15	theme	adsorbent	1184:1192	arg1	regeneration					1194:1205	the adsorbent regeneration	1180:1205	the adsorbent regeneration	1180:1205	Meanwhile, the mechanical resilience of the composite aerogels was significantly improved, facilitating the adsorbent regeneration by simple mechanical squeezing.
34323711	2	16	theme	hydrophobic	354:364	arg1	glutaraldehyde					524:537	glutaraldehyde	524:537	glutaraldehyde	524:537	Herein, hydrophobic aerogels were successfully fabricated from Pickering emulsions stabilized by chitin nanofibers (ChNF) with polydimethylsiloxane (PDMS) as dispersed phase and glutaraldehyde as a crosslinking agent, and their performance in HOCs removal were evaluated.
34323711	2	16	theme	hydrophobic	354:364	arg1	phase					514:518	dispersed phase	504:518	dispersed phase	504:518	Herein, hydrophobic aerogels were successfully fabricated from Pickering emulsions stabilized by chitin nanofibers (ChNF) with polydimethylsiloxane (PDMS) as dispersed phase and glutaraldehyde as a crosslinking agent, and their performance in HOCs removal were evaluated.
34323711	2	16	theme	hydrophobic	354:364	arg1	aerogels					366:373	hydrophobic aerogels	354:373	hydrophobic aerogels	354:373	Herein, hydrophobic aerogels were successfully fabricated from Pickering emulsions stabilized by chitin nanofibers (ChNF) with polydimethylsiloxane (PDMS) as dispersed phase and glutaraldehyde as a crosslinking agent, and their performance in HOCs removal were evaluated.
34323711	2	16	theme	hydrophobic	354:364	arg1	agent					557:561	a crosslinking agent	542:561	a crosslinking agent	542:561	Herein, hydrophobic aerogels were successfully fabricated from Pickering emulsions stabilized by chitin nanofibers (ChNF) with polydimethylsiloxane (PDMS) as dispersed phase and glutaraldehyde as a crosslinking agent, and their performance in HOCs removal were evaluated.
34323711	0	17	theme	organic	132:138	arg1	removal					152:158	hydrophobic organic contaminant removal	120:158	hydrophobic organic contaminant removal	120:158	Fabrication of chitin nanofiber-PDMS composite aerogels from Pickering emulsion templates with potential application in hydrophobic organic contaminant removal.
34323711	5	18	theme	phase	978:982	arg1	HOCs					984:987	non-aqueous phase HOCs	966:987	non-aqueous phase HOCs	966:987	The composite aerogels with water contact angles of over 130° could selectively remove non-aqueous phase HOCs from water.
34323711	0	19	theme	hydrophobic	120:130	arg1	removal					152:158	hydrophobic organic contaminant removal	120:158	hydrophobic organic contaminant removal	120:158	Fabrication of chitin nanofiber-PDMS composite aerogels from Pickering emulsion templates with potential application in hydrophobic organic contaminant removal.
34323711	1	20	contain	have	178:181	arg2	attention					202:210	aroused increasing attention	183:210	aroused increasing attention	183:210	Natural polymers have aroused increasing attention in water treatment but their application in removing hydrophobic organic contaminants (HOCs) was limited due to their hydrophilicity.
34323711	1	20	contain	have	178:181	arg1	polymers					169:176	Natural polymers	161:176	Natural polymers	161:176	Natural polymers have aroused increasing attention in water treatment but their application in removing hydrophobic organic contaminants (HOCs) was limited due to their hydrophilicity.
34323711	7	21	theme	simple	1210:1215	arg1	squeezing					1228:1236	simple mechanical squeezing	1210:1236	simple mechanical squeezing	1210:1236	Meanwhile, the mechanical resilience of the composite aerogels was significantly improved, facilitating the adsorbent regeneration by simple mechanical squeezing.
34323711	2	22	from	performance	574:584	arg1	removal					594:600	HOCs removal	589:600	HOCs removal	589:600	Herein, hydrophobic aerogels were successfully fabricated from Pickering emulsions stabilized by chitin nanofibers (ChNF) with polydimethylsiloxane (PDMS) as dispersed phase and glutaraldehyde as a crosslinking agent, and their performance in HOCs removal were evaluated.
34323711	2	23	theme	chitin	443:448	arg1	ChNF					462:465	ChNF	462:465	ChNF	462:465	Herein, hydrophobic aerogels were successfully fabricated from Pickering emulsions stabilized by chitin nanofibers (ChNF) with polydimethylsiloxane (PDMS) as dispersed phase and glutaraldehyde as a crosslinking agent, and their performance in HOCs removal were evaluated.
34323711	2	23	theme	chitin	443:448	arg1	nanofibers					450:459	chitin nanofibers	443:459	chitin nanofibers (ChNF) with polydimethylsiloxane (PDMS)	443:499	Herein, hydrophobic aerogels were successfully fabricated from Pickering emulsions stabilized by chitin nanofibers (ChNF) with polydimethylsiloxane (PDMS) as dispersed phase and glutaraldehyde as a crosslinking agent, and their performance in HOCs removal were evaluated.
34323711	10	24	theme	natural	1543:1549	arg1	biopolymers					1551:1561	natural biopolymers	1543:1561	natural biopolymers	1543:1561	This work reveals the potential of Pickering emulsions in the fabrication of composite hydrophobic materials from natural biopolymers with promising application in HOCs related water treatment.
34323711	9	25	theme	adsorption	1379:1388	arg1	capacity					1390:1397	a maximum adsorption capacity	1369:1397	a maximum adsorption capacity of 1.34 mg/g for 10 mg/L TCE	1369:1426	Moreover, the aerogels could also remove dissolved HOCs from water with a maximum adsorption capacity of 1.34 mg/g for 10 mg/L TCE.
34323711	1	26	theme	water	215:219	arg1	treatment					221:229	water treatment	215:229	water treatment	215:229	Natural polymers have aroused increasing attention in water treatment but their application in removing hydrophobic organic contaminants (HOCs) was limited due to their hydrophilicity.
34323711	9	27	theme	1.34 mg/g	1402:1410	arg1	capacity					1390:1397	a maximum adsorption capacity	1369:1397	a maximum adsorption capacity of 1.34 mg/g for 10 mg/L TCE	1369:1426	Moreover, the aerogels could also remove dissolved HOCs from water with a maximum adsorption capacity of 1.34 mg/g for 10 mg/L TCE.
34323711	4	28	theme	PDMS	764:767	arg1	droplets					769:776	The solidified PDMS droplets	749:776	The solidified PDMS droplets	749:776	The solidified PDMS droplets were evenly distributed within the matrix, contributing to homogeneous and permanent hydrophobicity.
34323711	10	29	theme	promising	1568:1576	arg1	application					1578:1588	promising application	1568:1588	promising application in HOCs related water treatment	1568:1620	This work reveals the potential of Pickering emulsions in the fabrication of composite hydrophobic materials from natural biopolymers with promising application in HOCs related water treatment.
34323711	7	30	theme	aerogels	1130:1137	arg1	resilience					1102:1111	the mechanical resilience	1087:1111	the mechanical resilience of the composite aerogels	1087:1137	Meanwhile, the mechanical resilience of the composite aerogels was significantly improved, facilitating the adsorbent regeneration by simple mechanical squeezing.
34323711	7	30	theme	aerogels	1130:1137	arg1	Meanwhile					1076:1084	Meanwhile	1076:1084	Meanwhile	1076:1084	Meanwhile, the mechanical resilience of the composite aerogels was significantly improved, facilitating the adsorbent regeneration by simple mechanical squeezing.
34323711	10	31	theme	emulsions	1474:1482	arg1	potential					1451:1459	the potential	1447:1459	the potential of Pickering emulsions in the fabrication of composite hydrophobic materials from natural biopolymers	1447:1561	This work reveals the potential of Pickering emulsions in the fabrication of composite hydrophobic materials from natural biopolymers with promising application in HOCs related water treatment.
34323711	0	32	theme	chitin	15:20	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of chitin	0:20	Fabrication of chitin nanofiber-PDMS composite aerogels from Pickering emulsion templates with potential application in hydrophobic organic contaminant removal.
34323711	9	33	theme	10 mg/L	1416:1422	arg1	TCE					1424:1426	10 mg/L TCE	1416:1426	10 mg/L TCE	1416:1426	Moreover, the aerogels could also remove dissolved HOCs from water with a maximum adsorption capacity of 1.34 mg/g for 10 mg/L TCE.
34323711	0	34	theme	contaminant	140:150	arg1	removal					152:158	hydrophobic organic contaminant removal	120:158	hydrophobic organic contaminant removal	120:158	Fabrication of chitin nanofiber-PDMS composite aerogels from Pickering emulsion templates with potential application in hydrophobic organic contaminant removal.
34323711	3	35	theme	high	681:684	arg1	stability					686:694	high stability	681:694	high stability	681:694	The Pickering emulsions with PDMS ratios of 2.5-20% v/v showed high stability, demonstrating great potential as aerogel templates.
34323711	5	36	theme	non-aqueous	966:976	arg1	HOCs					984:987	non-aqueous phase HOCs	966:987	non-aqueous phase HOCs	966:987	The composite aerogels with water contact angles of over 130° could selectively remove non-aqueous phase HOCs from water.
34323711	5	37	with	aerogels	893:900	arg1	angles					921:926	water contact angles	907:926	water contact angles of over 130°	907:939	The composite aerogels with water contact angles of over 130° could selectively remove non-aqueous phase HOCs from water.
34323711	5	38	theme	water	907:911	arg1	angles					921:926	water contact angles	907:926	water contact angles of over 130°	907:939	The composite aerogels with water contact angles of over 130° could selectively remove non-aqueous phase HOCs from water.
34323711	0	39	theme	composite	37:45	arg1	aerogels					47:54	composite aerogels	37:54	composite aerogels from Pickering emulsion templates	37:88	Fabrication of chitin nanofiber-PDMS composite aerogels from Pickering emulsion templates with potential application in hydrophobic organic contaminant removal.
34323711	6	40	theme	adsorption	1010:1019	arg1	capacity					1021:1028	The CCl4 adsorption capacity	1001:1028	The CCl4 adsorption capacity	1001:1028	The CCl4 adsorption capacity was 521-2820 wt%, depending on PDMS contents.
34323711	6	40	theme	adsorption	1010:1019	arg1	%					1045:1045	521-2820 wt%	1034:1045	521-2820 wt%	1034:1045	The CCl4 adsorption capacity was 521-2820 wt%, depending on PDMS contents.
34323711	7	41	theme	composite	1120:1128	arg1	aerogels					1130:1137	the composite aerogels	1116:1137	the composite aerogels	1116:1137	Meanwhile, the mechanical resilience of the composite aerogels was significantly improved, facilitating the adsorbent regeneration by simple mechanical squeezing.
34323711	0	42	from	templates	80:88	arg1	aerogels					47:54	composite aerogels	37:54	composite aerogels from Pickering emulsion templates	37:88	Fabrication of chitin nanofiber-PDMS composite aerogels from Pickering emulsion templates with potential application in hydrophobic organic contaminant removal.
34323711	3	43	theme	PDMS	647:650	arg1	ratios					652:657	PDMS ratios	647:657	PDMS ratios of 2.5-20% v/v	647:672	The Pickering emulsions with PDMS ratios of 2.5-20% v/v showed high stability, demonstrating great potential as aerogel templates.
34323711	5	44	theme	contact	913:919	arg1	angles					921:926	water contact angles	907:926	water contact angles of over 130°	907:939	The composite aerogels with water contact angles of over 130° could selectively remove non-aqueous phase HOCs from water.
34323711	0	45	theme	Pickering	61:69	arg1	templates					80:88	Pickering emulsion templates	61:88	Pickering emulsion templates	61:88	Fabrication of chitin nanofiber-PDMS composite aerogels from Pickering emulsion templates with potential application in hydrophobic organic contaminant removal.
34323711	6	46	theme	CCl4	1005:1008	arg1	capacity					1021:1028	The CCl4 adsorption capacity	1001:1028	The CCl4 adsorption capacity	1001:1028	The CCl4 adsorption capacity was 521-2820 wt%, depending on PDMS contents.
34323711	6	46	theme	CCl4	1005:1008	arg1	%					1045:1045	521-2820 wt%	1034:1045	521-2820 wt%	1034:1045	The CCl4 adsorption capacity was 521-2820 wt%, depending on PDMS contents.
34323711	0	47	theme	emulsion	71:78	arg1	templates					80:88	Pickering emulsion templates	61:88	Pickering emulsion templates	61:88	Fabrication of chitin nanofiber-PDMS composite aerogels from Pickering emulsion templates with potential application in hydrophobic organic contaminant removal.
34323711	3	48	theme	Pickering	622:630	arg1	emulsions					632:640	The Pickering emulsions	618:640	The Pickering emulsions with PDMS ratios of 2.5-20% v/v	618:672	The Pickering emulsions with PDMS ratios of 2.5-20% v/v showed high stability, demonstrating great potential as aerogel templates.
34323711	4	49	theme	solidified	753:762	arg1	droplets					769:776	The solidified PDMS droplets	749:776	The solidified PDMS droplets	749:776	The solidified PDMS droplets were evenly distributed within the matrix, contributing to homogeneous and permanent hydrophobicity.
34323711	7	50	theme	mechanical	1217:1226	arg1	squeezing					1228:1236	simple mechanical squeezing	1210:1236	simple mechanical squeezing	1210:1236	Meanwhile, the mechanical resilience of the composite aerogels was significantly improved, facilitating the adsorbent regeneration by simple mechanical squeezing.
34323711	3	51	theme	great	711:715	arg1	templates					738:746	aerogel templates	730:746	aerogel templates	730:746	The Pickering emulsions with PDMS ratios of 2.5-20% v/v showed high stability, demonstrating great potential as aerogel templates.
34323711	3	51	theme	great	711:715	arg1	potential					717:725	great potential	711:725	great potential	711:725	The Pickering emulsions with PDMS ratios of 2.5-20% v/v showed high stability, demonstrating great potential as aerogel templates.
34323711	10	52	from	potential	1451:1459	arg1	biopolymers					1551:1561	natural biopolymers	1543:1561	natural biopolymers	1543:1561	This work reveals the potential of Pickering emulsions in the fabrication of composite hydrophobic materials from natural biopolymers with promising application in HOCs related water treatment.
34323711	10	52	from	potential	1451:1459	arg1	fabrication					1491:1501	the fabrication	1487:1501	the fabrication of composite hydrophobic materials from natural biopolymers	1487:1561	This work reveals the potential of Pickering emulsions in the fabrication of composite hydrophobic materials from natural biopolymers with promising application in HOCs related water treatment.
34323711	3	53	theme	v/v	670:672	arg1	ratios					652:657	PDMS ratios	647:657	PDMS ratios of 2.5-20% v/v	647:672	The Pickering emulsions with PDMS ratios of 2.5-20% v/v showed high stability, demonstrating great potential as aerogel templates.
34323711	10	54	from	biopolymers	1551:1561	arg1	potential					1451:1459	the potential	1447:1459	the potential of Pickering emulsions in the fabrication of composite hydrophobic materials from natural biopolymers	1447:1561	This work reveals the potential of Pickering emulsions in the fabrication of composite hydrophobic materials from natural biopolymers with promising application in HOCs related water treatment.
34323711	10	54	from	biopolymers	1551:1561	arg1	fabrication					1491:1501	the fabrication	1487:1501	the fabrication of composite hydrophobic materials from natural biopolymers	1487:1561	This work reveals the potential of Pickering emulsions in the fabrication of composite hydrophobic materials from natural biopolymers with promising application in HOCs related water treatment.
34323711	10	55	theme	water	1606:1610	arg1	treatment					1612:1620	HOCs related water treatment	1593:1620	HOCs related water treatment	1593:1620	This work reveals the potential of Pickering emulsions in the fabrication of composite hydrophobic materials from natural biopolymers with promising application in HOCs related water treatment.
34323711	4	56	theme	homogeneous	837:847	arg1	hydrophobicity					863:876	homogeneous and permanent hydrophobicity	837:876	homogeneous and permanent hydrophobicity	837:876	The solidified PDMS droplets were evenly distributed within the matrix, contributing to homogeneous and permanent hydrophobicity.
34323711	3	57	theme	2.5-20	662:667	arg1	%					668:668	%	668:668	%	668:668	The Pickering emulsions with PDMS ratios of 2.5-20% v/v showed high stability, demonstrating great potential as aerogel templates.
34323711	10	58	from	application	1578:1588	arg1	treatment					1612:1620	HOCs related water treatment	1593:1620	HOCs related water treatment	1593:1620	This work reveals the potential of Pickering emulsions in the fabrication of composite hydrophobic materials from natural biopolymers with promising application in HOCs related water treatment.
34323711	7	59	theme	mechanical	1091:1100	arg1	resilience					1102:1111	the mechanical resilience	1087:1111	the mechanical resilience of the composite aerogels	1087:1137	Meanwhile, the mechanical resilience of the composite aerogels was significantly improved, facilitating the adsorbent regeneration by simple mechanical squeezing.
34323711	7	59	theme	mechanical	1091:1100	arg1	Meanwhile					1076:1084	Meanwhile	1076:1084	Meanwhile	1076:1084	Meanwhile, the mechanical resilience of the composite aerogels was significantly improved, facilitating the adsorbent regeneration by simple mechanical squeezing.
34323711	9	60	theme	dissolved	1338:1346	arg1	HOCs					1348:1351	dissolved HOCs	1338:1351	dissolved HOCs	1338:1351	Moreover, the aerogels could also remove dissolved HOCs from water with a maximum adsorption capacity of 1.34 mg/g for 10 mg/L TCE.
34323711	3	61	theme	%	668:668	arg1	v/v					670:672	2.5-20% v/v	662:672	2.5-20% v/v	662:672	The Pickering emulsions with PDMS ratios of 2.5-20% v/v showed high stability, demonstrating great potential as aerogel templates.
34323711	1	62	theme	Natural	161:167	arg1	polymers					169:176	Natural polymers	161:176	Natural polymers	161:176	Natural polymers have aroused increasing attention in water treatment but their application in removing hydrophobic organic contaminants (HOCs) was limited due to their hydrophilicity.
34323711	1	63	theme	hydrophobic	265:275	arg1	HOCs					299:302	HOCs	299:302	HOCs	299:302	Natural polymers have aroused increasing attention in water treatment but their application in removing hydrophobic organic contaminants (HOCs) was limited due to their hydrophilicity.
34323711	1	63	theme	hydrophobic	265:275	arg1	contaminants					285:296	hydrophobic organic contaminants	265:296	hydrophobic organic contaminants (HOCs)	265:303	Natural polymers have aroused increasing attention in water treatment but their application in removing hydrophobic organic contaminants (HOCs) was limited due to their hydrophilicity.
34323711	2	64	theme	crosslinking	544:555	arg1	aerogels					366:373	hydrophobic aerogels	354:373	hydrophobic aerogels	354:373	Herein, hydrophobic aerogels were successfully fabricated from Pickering emulsions stabilized by chitin nanofibers (ChNF) with polydimethylsiloxane (PDMS) as dispersed phase and glutaraldehyde as a crosslinking agent, and their performance in HOCs removal were evaluated.
34323711	2	64	theme	crosslinking	544:555	arg1	agent					557:561	a crosslinking agent	542:561	a crosslinking agent	542:561	Herein, hydrophobic aerogels were successfully fabricated from Pickering emulsions stabilized by chitin nanofibers (ChNF) with polydimethylsiloxane (PDMS) as dispersed phase and glutaraldehyde as a crosslinking agent, and their performance in HOCs removal were evaluated.
34323711	0	65	from	application	105:115	arg1	removal					152:158	hydrophobic organic contaminant removal	120:158	hydrophobic organic contaminant removal	120:158	Fabrication of chitin nanofiber-PDMS composite aerogels from Pickering emulsion templates with potential application in hydrophobic organic contaminant removal.
34323711	4	66	theme	permanent	853:861	arg1	hydrophobicity					863:876	homogeneous and permanent hydrophobicity	837:876	homogeneous and permanent hydrophobicity	837:876	The solidified PDMS droplets were evenly distributed within the matrix, contributing to homogeneous and permanent hydrophobicity.
34323711	1	67	theme	organic	277:283	arg1	HOCs					299:302	HOCs	299:302	HOCs	299:302	Natural polymers have aroused increasing attention in water treatment but their application in removing hydrophobic organic contaminants (HOCs) was limited due to their hydrophilicity.
34323711	1	67	theme	organic	277:283	arg1	contaminants					285:296	hydrophobic organic contaminants	265:296	hydrophobic organic contaminants (HOCs)	265:303	Natural polymers have aroused increasing attention in water treatment but their application in removing hydrophobic organic contaminants (HOCs) was limited due to their hydrophilicity.
34323711	2	68	theme	dispersed	504:512	arg1	phase					514:518	dispersed phase	504:518	dispersed phase	504:518	Herein, hydrophobic aerogels were successfully fabricated from Pickering emulsions stabilized by chitin nanofibers (ChNF) with polydimethylsiloxane (PDMS) as dispersed phase and glutaraldehyde as a crosslinking agent, and their performance in HOCs removal were evaluated.
34323711	2	68	theme	dispersed	504:512	arg1	aerogels					366:373	hydrophobic aerogels	354:373	hydrophobic aerogels	354:373	Herein, hydrophobic aerogels were successfully fabricated from Pickering emulsions stabilized by chitin nanofibers (ChNF) with polydimethylsiloxane (PDMS) as dispersed phase and glutaraldehyde as a crosslinking agent, and their performance in HOCs removal were evaluated.
34323711	5	69	theme	composite	883:891	arg1	aerogels					893:900	The composite aerogels	879:900	The composite aerogels with water contact angles of over 130°	879:939	The composite aerogels with water contact angles of over 130° could selectively remove non-aqueous phase HOCs from water.
34323711	2	70	theme	Pickering	409:417	arg1	emulsions					419:427	Pickering emulsions	409:427	Pickering emulsions stabilized by chitin nanofibers (ChNF) with polydimethylsiloxane (PDMS)	409:499	Herein, hydrophobic aerogels were successfully fabricated from Pickering emulsions stabilized by chitin nanofibers (ChNF) with polydimethylsiloxane (PDMS) as dispersed phase and glutaraldehyde as a crosslinking agent, and their performance in HOCs removal were evaluated.
34536942	6	0	theme	adsorption	1006:1015	arg1	behavior					1017:1024	the fundamental adsorption behavior	990:1024	the fundamental adsorption behavior	990:1024	Furthermore, isothermal, kinetic and thermodynamic studies were performed to explore the fundamental adsorption behavior.
34536942	7	1	theme	Freundlich	1032:1041	arg1	isotherm					1043:1050	Freundlich isotherm	1032:1050	Freundlich isotherm	1032:1050	Both Freundlich isotherm and Langmuir isotherm models can fit the adsorption isotherm data well, and the adsorption kinetics is well described by Pseudo-second-order.
34536942	1	2	theme	microporosity	176:188	arg1	Polymers					154:161	Polymers	154:161	Polymers of intrinsic microporosity (PIM-1)	154:196	Polymers of intrinsic microporosity (PIM-1) has demonstrated great potential in adsorption and separation fields.
34536942	11	3	theme	cationic	1707:1714	arg1	dyes					1716:1719	cationic dyes	1707:1719	cationic dyes	1707:1719	These findings point that AOPIM-1/Alg composite hydrogel beads are an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution.
34536942	0	4	theme	efficient	93:101	arg1	adsorption					103:112	efficient adsorption	93:112	efficient adsorption of cationic dyes from aqueous solution	93:151	Amidoxime modified polymers of intrinsic microporosity/alginate composite hydrogel beads for efficient adsorption of cationic dyes from aqueous solution.
34536942	4	5	theme	doped	662:666	arg1	ratio					668:672	doped ratio	662:672	doped ratio	662:672	The AOPIM-1/Alg composite beads were further employed for removal of malachite green (MG) from aqueous solution and the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance were systematically investigated.
34536942	10	6	theme	composite	1504:1512	arg1	beads					1514:1518	the composite beads	1500:1518	the composite beads	1500:1518	The regeneration test shows that above 90% of the adsorption capacity of the composite beads can be maintained after 10 cycles of MG adsorption/desorption.
34536942	11	7	theme	aqueous	1737:1743	arg1	solution					1745:1752	aqueous solution	1737:1752	aqueous solution	1737:1752	These findings point that AOPIM-1/Alg composite hydrogel beads are an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution.
34536942	0	8	theme	cationic	117:124	arg1	dyes					126:129	cationic dyes	117:129	cationic dyes from aqueous solution	117:151	Amidoxime modified polymers of intrinsic microporosity/alginate composite hydrogel beads for efficient adsorption of cationic dyes from aqueous solution.
34536942	11	9	theme	up-and-coming	1664:1676	arg1	adsorption					1721:1730	an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption	1650:1730	an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution	1650:1752	These findings point that AOPIM-1/Alg composite hydrogel beads are an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution.
34536942	11	9	theme	up-and-coming	1664:1676	arg1	beads					1640:1644	AOPIM-1/Alg composite hydrogel beads	1609:1644	AOPIM-1/Alg composite hydrogel beads	1609:1644	These findings point that AOPIM-1/Alg composite hydrogel beads are an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution.
34536942	4	10	theme	dye	719:721	arg1	concentration					723:735	initial dye concentration	711:735	initial dye concentration	711:735	The AOPIM-1/Alg composite beads were further employed for removal of malachite green (MG) from aqueous solution and the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance were systematically investigated.
34536942	4	11	from	effects	651:657	arg1	performance					758:768	the MG adsorption performance	740:768	the MG adsorption performance	740:768	The AOPIM-1/Alg composite beads were further employed for removal of malachite green (MG) from aqueous solution and the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance were systematically investigated.
34536942	9	12	theme	AOPIM-1/Alg	1321:1331	arg1	beads					1333:1337	AOPIM-1/Alg beads	1321:1337	AOPIM-1/Alg beads	1321:1337	In addition, mixed dyes adsorption measurements indicate that AOPIM-1/Alg beads are highly selective to adsorb cationic dyes from anionic/cationic mixed dyes solution.
34536942	4	13	theme	initial	711:717	arg1	concentration					723:735	initial dye concentration	711:735	initial dye concentration	711:735	The AOPIM-1/Alg composite beads were further employed for removal of malachite green (MG) from aqueous solution and the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance were systematically investigated.
34536942	4	14	theme	ratio	668:672	arg1	effects					651:657	the effects	647:657	the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance	647:768	The AOPIM-1/Alg composite beads were further employed for removal of malachite green (MG) from aqueous solution and the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance were systematically investigated.
34536942	4	15	theme	dosage	685:690	arg1	effects					651:657	the effects	647:657	the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance	647:768	The AOPIM-1/Alg composite beads were further employed for removal of malachite green (MG) from aqueous solution and the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance were systematically investigated.
34536942	11	16	theme	recyclable	1682:1691	arg1	adsorption					1721:1730	an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption	1650:1730	an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution	1650:1752	These findings point that AOPIM-1/Alg composite hydrogel beads are an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution.
34536942	11	16	theme	recyclable	1682:1691	arg1	beads					1640:1644	AOPIM-1/Alg composite hydrogel beads	1609:1644	AOPIM-1/Alg composite hydrogel beads	1609:1644	These findings point that AOPIM-1/Alg composite hydrogel beads are an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution.
34536942	4	17	theme	adsorbent	675:683	arg1	dosage					685:690	adsorbent dosage	675:690	adsorbent dosage	675:690	The AOPIM-1/Alg composite beads were further employed for removal of malachite green (MG) from aqueous solution and the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance were systematically investigated.
34536942	7	18	theme	adsorption	1093:1102	arg1	data					1113:1116	the adsorption isotherm data	1089:1116	the adsorption isotherm data	1089:1116	Both Freundlich isotherm and Langmuir isotherm models can fit the adsorption isotherm data well, and the adsorption kinetics is well described by Pseudo-second-order.
34536942	3	19	theme	composite	505:513	arg1	beads					524:528	composite hydrogel beads	505:528	composite hydrogel beads	505:528	PIM-1 was first modified by amidoxime, and then the amidoxime modified PIM-1 (AOPIM-1) was mingled into alginate (Alg) hydrogel to obtain composite hydrogel beads.
34536942	5	20	theme	Alg	839:841	arg1	beads					843:847	pure Alg beads	834:847	pure Alg beads	834:847	The MG adsorption capacity of pure Alg beads was substantially enhanced after incorporating AOPIM-1.
34536942	0	21	theme	dyes	126:129	arg1	adsorption					103:112	efficient adsorption	93:112	efficient adsorption of cationic dyes from aqueous solution	93:151	Amidoxime modified polymers of intrinsic microporosity/alginate composite hydrogel beads for efficient adsorption of cationic dyes from aqueous solution.
34536942	11	22	theme	adsorbent	1693:1701	arg1	adsorption					1721:1730	an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption	1650:1730	an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution	1650:1752	These findings point that AOPIM-1/Alg composite hydrogel beads are an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution.
34536942	11	22	theme	adsorbent	1693:1701	arg1	beads					1640:1644	AOPIM-1/Alg composite hydrogel beads	1609:1644	AOPIM-1/Alg composite hydrogel beads	1609:1644	These findings point that AOPIM-1/Alg composite hydrogel beads are an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution.
34536942	7	23	theme	isotherm	1104:1111	arg1	data					1113:1116	the adsorption isotherm data	1089:1116	the adsorption isotherm data	1089:1116	Both Freundlich isotherm and Langmuir isotherm models can fit the adsorption isotherm data well, and the adsorption kinetics is well described by Pseudo-second-order.
34536942	3	24	theme	hydrogel	515:522	arg1	beads					524:528	composite hydrogel beads	505:528	composite hydrogel beads	505:528	PIM-1 was first modified by amidoxime, and then the amidoxime modified PIM-1 (AOPIM-1) was mingled into alginate (Alg) hydrogel to obtain composite hydrogel beads.
34536942	4	25	from	solution	634:641	arg1	removal					589:595	removal	589:595	removal of malachite green (MG) from aqueous solution	589:641	The AOPIM-1/Alg composite beads were further employed for removal of malachite green (MG) from aqueous solution and the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance were systematically investigated.
34536942	4	26	theme	aqueous	626:632	arg1	solution					634:641	aqueous solution	626:641	aqueous solution	626:641	The AOPIM-1/Alg composite beads were further employed for removal of malachite green (MG) from aqueous solution and the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance were systematically investigated.
34536942	0	27	theme	modified	10:17	arg1	polymers					19:26	Amidoxime modified polymers	0:26	Amidoxime modified polymers of intrinsic	0:39	Amidoxime modified polymers of intrinsic microporosity/alginate composite hydrogel beads for efficient adsorption of cationic dyes from aqueous solution.
34536942	9	28	theme	cationic	1370:1377	arg1	dyes					1379:1382	cationic dyes	1370:1382	cationic dyes from anionic/cationic mixed dyes solution	1370:1424	In addition, mixed dyes adsorption measurements indicate that AOPIM-1/Alg beads are highly selective to adsorb cationic dyes from anionic/cationic mixed dyes solution.
34536942	10	29	theme	adsorption	1477:1486	arg1	capacity					1488:1495	the adsorption capacity	1473:1495	the adsorption capacity of the composite beads	1473:1518	The regeneration test shows that above 90% of the adsorption capacity of the composite beads can be maintained after 10 cycles of MG adsorption/desorption.
34536942	10	30	theme	adsorption/desorption	1560:1580	arg1	cycles					1547:1552	10 cycles	1544:1552	10 cycles of MG adsorption/desorption	1544:1580	The regeneration test shows that above 90% of the adsorption capacity of the composite beads can be maintained after 10 cycles of MG adsorption/desorption.
34536942	4	31	theme	time	701:704	arg1	effects					651:657	the effects	647:657	the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance	647:768	The AOPIM-1/Alg composite beads were further employed for removal of malachite green (MG) from aqueous solution and the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance were systematically investigated.
34536942	3	32	theme	amidoxime	419:427	arg1	PIM-1					438:442	then the amidoxime modified PIM-1	410:442	then the amidoxime modified PIM-1 (AOPIM-1)	410:452	PIM-1 was first modified by amidoxime, and then the amidoxime modified PIM-1 (AOPIM-1) was mingled into alginate (Alg) hydrogel to obtain composite hydrogel beads.
34536942	3	32	theme	amidoxime	419:427	arg1	AOPIM-1					445:451	AOPIM-1	445:451	AOPIM-1	445:451	PIM-1 was first modified by amidoxime, and then the amidoxime modified PIM-1 (AOPIM-1) was mingled into alginate (Alg) hydrogel to obtain composite hydrogel beads.
34536942	0	33	theme	Amidoxime	0:8	arg1	polymers					19:26	Amidoxime modified polymers	0:26	Amidoxime modified polymers of intrinsic	0:39	Amidoxime modified polymers of intrinsic microporosity/alginate composite hydrogel beads for efficient adsorption of cationic dyes from aqueous solution.
34536942	4	34	theme	AOPIM-1/Alg	535:545	arg1	beads					557:561	The AOPIM-1/Alg composite beads	531:561	The AOPIM-1/Alg composite beads	531:561	The AOPIM-1/Alg composite beads were further employed for removal of malachite green (MG) from aqueous solution and the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance were systematically investigated.
34536942	9	35	theme	anionic/cationic	1389:1404	arg1	solution					1417:1424	anionic/cationic mixed dyes solution	1389:1424	anionic/cationic mixed dyes solution	1389:1424	In addition, mixed dyes adsorption measurements indicate that AOPIM-1/Alg beads are highly selective to adsorb cationic dyes from anionic/cationic mixed dyes solution.
34536942	4	36	theme	contact	693:699	arg1	time					701:704	contact time	693:704	contact time	693:704	The AOPIM-1/Alg composite beads were further employed for removal of malachite green (MG) from aqueous solution and the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance were systematically investigated.
34536942	3	37	theme	modified	429:436	arg1	PIM-1					438:442	then the amidoxime modified PIM-1	410:442	then the amidoxime modified PIM-1 (AOPIM-1)	410:452	PIM-1 was first modified by amidoxime, and then the amidoxime modified PIM-1 (AOPIM-1) was mingled into alginate (Alg) hydrogel to obtain composite hydrogel beads.
34536942	3	37	theme	modified	429:436	arg1	AOPIM-1					445:451	AOPIM-1	445:451	AOPIM-1	445:451	PIM-1 was first modified by amidoxime, and then the amidoxime modified PIM-1 (AOPIM-1) was mingled into alginate (Alg) hydrogel to obtain composite hydrogel beads.
34536942	10	38	theme	regeneration	1431:1442	arg1	test					1444:1447	The regeneration test	1427:1447	The regeneration test	1427:1447	The regeneration test shows that above 90% of the adsorption capacity of the composite beads can be maintained after 10 cycles of MG adsorption/desorption.
34536942	6	39	theme	fundamental	994:1004	arg1	behavior					1017:1024	the fundamental adsorption behavior	990:1024	the fundamental adsorption behavior	990:1024	Furthermore, isothermal, kinetic and thermodynamic studies were performed to explore the fundamental adsorption behavior.
34536942	1	40	theme	great	215:219	arg1	potential					221:229	great potential	215:229	great potential	215:229	Polymers of intrinsic microporosity (PIM-1) has demonstrated great potential in adsorption and separation fields.
34536942	8	41	theme	adsorption	1198:1207	arg1	feasible					1220:1227	feasible	1220:1227	feasible	1220:1227	The adsorption process is feasible, spontaneous and endothermic.
34536942	8	41	theme	adsorption	1198:1207	arg1	process					1209:1215	The adsorption process	1194:1215	The adsorption process	1194:1215	The adsorption process is feasible, spontaneous and endothermic.
34536942	6	42	theme	thermodynamic	942:954	arg1	studies					956:962	isothermal, kinetic and thermodynamic studies	918:962	isothermal, kinetic and thermodynamic studies	918:962	Furthermore, isothermal, kinetic and thermodynamic studies were performed to explore the fundamental adsorption behavior.
34536942	0	43	theme	aqueous	136:142	arg1	solution					144:151	aqueous solution	136:151	aqueous solution	136:151	Amidoxime modified polymers of intrinsic microporosity/alginate composite hydrogel beads for efficient adsorption of cationic dyes from aqueous solution.
34536942	0	44	from	adsorption	103:112	arg1	solution					144:151	aqueous solution	136:151	aqueous solution	136:151	Amidoxime modified polymers of intrinsic microporosity/alginate composite hydrogel beads for efficient adsorption of cationic dyes from aqueous solution.
34536942	5	45	theme	MG	808:809	arg1	capacity					822:829	The MG adsorption capacity	804:829	The MG adsorption capacity of pure Alg beads	804:847	The MG adsorption capacity of pure Alg beads was substantially enhanced after incorporating AOPIM-1.
34536942	4	46	theme	composite	547:555	arg1	beads					557:561	The AOPIM-1/Alg composite beads	531:561	The AOPIM-1/Alg composite beads	531:561	The AOPIM-1/Alg composite beads were further employed for removal of malachite green (MG) from aqueous solution and the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance were systematically investigated.
34536942	7	47	theme	Langmuir	1056:1063	arg1	isotherm					1065:1072	Langmuir isotherm	1056:1072	Langmuir isotherm	1056:1072	Both Freundlich isotherm and Langmuir isotherm models can fit the adsorption isotherm data well, and the adsorption kinetics is well described by Pseudo-second-order.
34536942	5	48	theme	beads	843:847	arg1	capacity					822:829	The MG adsorption capacity	804:829	The MG adsorption capacity of pure Alg beads	804:847	The MG adsorption capacity of pure Alg beads was substantially enhanced after incorporating AOPIM-1.
34536942	5	49	theme	adsorption	811:820	arg1	capacity					822:829	The MG adsorption capacity	804:829	The MG adsorption capacity of pure Alg beads	804:847	The MG adsorption capacity of pure Alg beads was substantially enhanced after incorporating AOPIM-1.
34536942	0	50	theme	intrinsic	31:39	arg1	polymers					19:26	Amidoxime modified polymers	0:26	Amidoxime modified polymers of intrinsic	0:39	Amidoxime modified polymers of intrinsic microporosity/alginate composite hydrogel beads for efficient adsorption of cationic dyes from aqueous solution.
34536942	6	51	theme	kinetic	930:936	arg1	studies					956:962	isothermal, kinetic and thermodynamic studies	918:962	isothermal, kinetic and thermodynamic studies	918:962	Furthermore, isothermal, kinetic and thermodynamic studies were performed to explore the fundamental adsorption behavior.
34536942	7	52	theme	isotherm	1065:1072	arg1	models					1074:1079	Both Freundlich isotherm and Langmuir isotherm models	1027:1079	Both Freundlich isotherm and Langmuir isotherm models	1027:1079	Both Freundlich isotherm and Langmuir isotherm models can fit the adsorption isotherm data well, and the adsorption kinetics is well described by Pseudo-second-order.
34536942	4	53	theme	green	610:614	arg1	removal					589:595	removal	589:595	removal of malachite green (MG) from aqueous solution	589:641	The AOPIM-1/Alg composite beads were further employed for removal of malachite green (MG) from aqueous solution and the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance were systematically investigated.
34536942	11	54	theme	composite	1621:1629	arg1	adsorption					1721:1730	an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption	1650:1730	an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution	1650:1752	These findings point that AOPIM-1/Alg composite hydrogel beads are an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution.
34536942	11	54	theme	composite	1621:1629	arg1	beads					1640:1644	AOPIM-1/Alg composite hydrogel beads	1609:1644	AOPIM-1/Alg composite hydrogel beads	1609:1644	These findings point that AOPIM-1/Alg composite hydrogel beads are an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution.
34536942	11	55	from	solution	1745:1752	arg1	adsorption					1721:1730	an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption	1650:1730	an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution	1650:1752	These findings point that AOPIM-1/Alg composite hydrogel beads are an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution.
34536942	11	55	from	solution	1745:1752	arg1	beads					1640:1644	AOPIM-1/Alg composite hydrogel beads	1609:1644	AOPIM-1/Alg composite hydrogel beads	1609:1644	These findings point that AOPIM-1/Alg composite hydrogel beads are an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution.
34536942	0	56	theme	hydrogel	74:81	arg1	beads					83:87	composite hydrogel beads	64:87	composite hydrogel beads for efficient adsorption of cationic dyes from aqueous solution	64:151	Amidoxime modified polymers of intrinsic microporosity/alginate composite hydrogel beads for efficient adsorption of cationic dyes from aqueous solution.
34536942	3	57	mod	modified	383:390	arg1	PIM-1					367:371	PIM-1	367:371	PIM-1	367:371	PIM-1 was first modified by amidoxime, and then the amidoxime modified PIM-1 (AOPIM-1) was mingled into alginate (Alg) hydrogel to obtain composite hydrogel beads.
34536942	3	57	mod	modified	383:390	arg3	amidoxime					395:403	amidoxime	395:403	amidoxime	395:403	PIM-1 was first modified by amidoxime, and then the amidoxime modified PIM-1 (AOPIM-1) was mingled into alginate (Alg) hydrogel to obtain composite hydrogel beads.
34536942	7	58	theme	isotherm	1043:1050	arg1	models					1074:1079	Both Freundlich isotherm and Langmuir isotherm models	1027:1079	Both Freundlich isotherm and Langmuir isotherm models	1027:1079	Both Freundlich isotherm and Langmuir isotherm models can fit the adsorption isotherm data well, and the adsorption kinetics is well described by Pseudo-second-order.
34536942	11	59	theme	hydrogel	1631:1638	arg1	adsorption					1721:1730	an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption	1650:1730	an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution	1650:1752	These findings point that AOPIM-1/Alg composite hydrogel beads are an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution.
34536942	11	59	theme	hydrogel	1631:1638	arg1	beads					1640:1644	AOPIM-1/Alg composite hydrogel beads	1609:1644	AOPIM-1/Alg composite hydrogel beads	1609:1644	These findings point that AOPIM-1/Alg composite hydrogel beads are an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution.
34536942	2	60	theme	facile	355:360	arg1	way					362:364	a facile way	353:364	a facile way	353:364	In this study, PIM-1 was structured into an applicable and efficient adsorbent using a facile way.
34536942	0	61	theme	composite	64:72	arg1	beads					83:87	composite hydrogel beads	64:87	composite hydrogel beads for efficient adsorption of cationic dyes from aqueous solution	64:151	Amidoxime modified polymers of intrinsic microporosity/alginate composite hydrogel beads for efficient adsorption of cationic dyes from aqueous solution.
34536942	6	62	theme	isothermal	918:927	arg1	studies					956:962	isothermal, kinetic and thermodynamic studies	918:962	isothermal, kinetic and thermodynamic studies	918:962	Furthermore, isothermal, kinetic and thermodynamic studies were performed to explore the fundamental adsorption behavior.
34536942	10	63	theme	capacity	1488:1495	arg1	%					1468:1468	above 90%	1460:1468	above 90% of the adsorption capacity of the composite beads	1460:1518	The regeneration test shows that above 90% of the adsorption capacity of the composite beads can be maintained after 10 cycles of MG adsorption/desorption.
34536942	10	63	theme	capacity	1488:1495	arg1	capacity					1488:1495	the adsorption capacity	1473:1495	the adsorption capacity of the composite beads	1473:1518	The regeneration test shows that above 90% of the adsorption capacity of the composite beads can be maintained after 10 cycles of MG adsorption/desorption.
34536942	3	64	theme	Alg	481:483	arg1	hydrogel					486:493	alginate (Alg) hydrogel	471:493	alginate (Alg) hydrogel	471:493	PIM-1 was first modified by amidoxime, and then the amidoxime modified PIM-1 (AOPIM-1) was mingled into alginate (Alg) hydrogel to obtain composite hydrogel beads.
34536942	9	65	theme	mixed	1406:1410	arg1	solution					1417:1424	anionic/cationic mixed dyes solution	1389:1424	anionic/cationic mixed dyes solution	1389:1424	In addition, mixed dyes adsorption measurements indicate that AOPIM-1/Alg beads are highly selective to adsorb cationic dyes from anionic/cationic mixed dyes solution.
34536942	7	66	theme	adsorption	1132:1141	arg1	kinetics					1143:1150	the adsorption kinetics	1128:1150	the adsorption kinetics	1128:1150	Both Freundlich isotherm and Langmuir isotherm models can fit the adsorption isotherm data well, and the adsorption kinetics is well described by Pseudo-second-order.
34536942	4	67	theme	MG	744:745	arg1	performance					758:768	the MG adsorption performance	740:768	the MG adsorption performance	740:768	The AOPIM-1/Alg composite beads were further employed for removal of malachite green (MG) from aqueous solution and the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance were systematically investigated.
34536942	11	68	theme	AOPIM-1/Alg	1609:1619	arg1	adsorption					1721:1730	an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption	1650:1730	an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution	1650:1752	These findings point that AOPIM-1/Alg composite hydrogel beads are an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution.
34536942	11	68	theme	AOPIM-1/Alg	1609:1619	arg1	beads					1640:1644	AOPIM-1/Alg composite hydrogel beads	1609:1644	AOPIM-1/Alg composite hydrogel beads	1609:1644	These findings point that AOPIM-1/Alg composite hydrogel beads are an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution.
34536942	10	69	theme	beads	1514:1518	arg1	capacity					1488:1495	the adsorption capacity	1473:1495	the adsorption capacity of the composite beads	1473:1518	The regeneration test shows that above 90% of the adsorption capacity of the composite beads can be maintained after 10 cycles of MG adsorption/desorption.
34536942	9	70	theme	dyes	1412:1415	arg1	solution					1417:1424	anionic/cationic mixed dyes solution	1389:1424	anionic/cationic mixed dyes solution	1389:1424	In addition, mixed dyes adsorption measurements indicate that AOPIM-1/Alg beads are highly selective to adsorb cationic dyes from anionic/cationic mixed dyes solution.
34536942	4	71	theme	concentration	723:735	arg1	effects					651:657	the effects	647:657	the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance	647:768	The AOPIM-1/Alg composite beads were further employed for removal of malachite green (MG) from aqueous solution and the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance were systematically investigated.
34536942	9	72	theme	mixed	1272:1276	arg1	measurements					1294:1305	mixed dyes adsorption measurements	1272:1305	mixed dyes adsorption measurements	1272:1305	In addition, mixed dyes adsorption measurements indicate that AOPIM-1/Alg beads are highly selective to adsorb cationic dyes from anionic/cationic mixed dyes solution.
34536942	3	73	theme	alginate	471:478	arg1	hydrogel					486:493	alginate (Alg) hydrogel	471:493	alginate (Alg) hydrogel	471:493	PIM-1 was first modified by amidoxime, and then the amidoxime modified PIM-1 (AOPIM-1) was mingled into alginate (Alg) hydrogel to obtain composite hydrogel beads.
34536942	1	74	theme	separation	249:258	arg1	fields					260:265	separation fields	249:265	separation fields	249:265	Polymers of intrinsic microporosity (PIM-1) has demonstrated great potential in adsorption and separation fields.
34536942	9	75	from	solution	1417:1424	arg1	dyes					1379:1382	cationic dyes	1370:1382	cationic dyes from anionic/cationic mixed dyes solution	1370:1424	In addition, mixed dyes adsorption measurements indicate that AOPIM-1/Alg beads are highly selective to adsorb cationic dyes from anionic/cationic mixed dyes solution.
34536942	9	76	theme	dyes	1278:1281	arg1	measurements					1294:1305	mixed dyes adsorption measurements	1272:1305	mixed dyes adsorption measurements	1272:1305	In addition, mixed dyes adsorption measurements indicate that AOPIM-1/Alg beads are highly selective to adsorb cationic dyes from anionic/cationic mixed dyes solution.
34536942	11	77	theme	efficient	1653:1661	arg1	adsorption					1721:1730	an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption	1650:1730	an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution	1650:1752	These findings point that AOPIM-1/Alg composite hydrogel beads are an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution.
34536942	11	77	theme	efficient	1653:1661	arg1	beads					1640:1644	AOPIM-1/Alg composite hydrogel beads	1609:1644	AOPIM-1/Alg composite hydrogel beads	1609:1644	These findings point that AOPIM-1/Alg composite hydrogel beads are an efficient, up-and-coming and recyclable adsorbent for cationic dyes adsorption from aqueous solution.
34536942	5	78	theme	pure	834:837	arg1	beads					843:847	pure Alg beads	834:847	pure Alg beads	834:847	The MG adsorption capacity of pure Alg beads was substantially enhanced after incorporating AOPIM-1.
34536942	0	79	from	solution	144:151	arg1	adsorption					103:112	efficient adsorption	93:112	efficient adsorption of cationic dyes from aqueous solution	93:151	Amidoxime modified polymers of intrinsic microporosity/alginate composite hydrogel beads for efficient adsorption of cationic dyes from aqueous solution.
34536942	0	79	from	solution	144:151	arg1	dyes					126:129	cationic dyes	117:129	cationic dyes from aqueous solution	117:151	Amidoxime modified polymers of intrinsic microporosity/alginate composite hydrogel beads for efficient adsorption of cationic dyes from aqueous solution.
34536942	10	80	theme	MG	1557:1558	arg1	adsorption/desorption					1560:1580	MG adsorption/desorption	1557:1580	MG adsorption/desorption	1557:1580	The regeneration test shows that above 90% of the adsorption capacity of the composite beads can be maintained after 10 cycles of MG adsorption/desorption.
34536942	9	81	theme	adsorption	1283:1292	arg1	measurements					1294:1305	mixed dyes adsorption measurements	1272:1305	mixed dyes adsorption measurements	1272:1305	In addition, mixed dyes adsorption measurements indicate that AOPIM-1/Alg beads are highly selective to adsorb cationic dyes from anionic/cationic mixed dyes solution.
34536942	4	82	theme	adsorption	747:756	arg1	performance					758:768	the MG adsorption performance	740:768	the MG adsorption performance	740:768	The AOPIM-1/Alg composite beads were further employed for removal of malachite green (MG) from aqueous solution and the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance were systematically investigated.
34536942	1	83	theme	intrinsic	166:174	arg1	PIM-1					191:195	PIM-1	191:195	PIM-1	191:195	Polymers of intrinsic microporosity (PIM-1) has demonstrated great potential in adsorption and separation fields.
34536942	1	83	theme	intrinsic	166:174	arg1	microporosity					176:188	intrinsic microporosity	166:188	intrinsic microporosity (PIM-1)	166:196	Polymers of intrinsic microporosity (PIM-1) has demonstrated great potential in adsorption and separation fields.
34536942	4	84	theme	malachite	600:608	arg1	MG					617:618	MG	617:618	MG	617:618	The AOPIM-1/Alg composite beads were further employed for removal of malachite green (MG) from aqueous solution and the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance were systematically investigated.
34536942	4	84	theme	malachite	600:608	arg1	green					610:614	malachite green	600:614	malachite green (MG)	600:619	The AOPIM-1/Alg composite beads were further employed for removal of malachite green (MG) from aqueous solution and the effects of doped ratio, adsorbent dosage, contact time, and initial dye concentration on the MG adsorption performance were systematically investigated.
34352692	5	0	theme	9.2	610:612	arg1	%					613:613	%	613:613	%	613:613	This method showed that the samples varied from 1.9% to 9.2% branching.
34352692	9	1	contain	has	1046:1048	arg2	profile					1066:1072	a unique purity profile	1050:1072	a unique purity profile	1050:1072	Our findings confirm that each proprietary source of yeast β-glucan has a unique purity profile, branching, and linkage patterns that determine the chemical structure and composition.
34352692	9	1	contain	has	1046:1048	arg1	source					1021:1026	each proprietary source	1004:1026	each proprietary source of yeast β-glucan	1004:1044	Our findings confirm that each proprietary source of yeast β-glucan has a unique purity profile, branching, and linkage patterns that determine the chemical structure and composition.
34352692	9	1	contain	has	1046:1048	arg2	patterns					1098:1105	linkage patterns	1090:1105	linkage patterns	1090:1105	Our findings confirm that each proprietary source of yeast β-glucan has a unique purity profile, branching, and linkage patterns that determine the chemical structure and composition.
34352692	9	1	contain	has	1046:1048	arg2	branching					1075:1083	branching	1075:1083	branching	1075:1083	Our findings confirm that each proprietary source of yeast β-glucan has a unique purity profile, branching, and linkage patterns that determine the chemical structure and composition.
34352692	8	2	theme	quantitative	920:931	arg1	differences					933:943	quantitative differences	920:943	quantitative differences in the samples	920:958	The samples were also analyzed by FT-IR and 1HNMR spectroscopy but it was difficult to derive quantitative differences in the samples by these methods.
34352692	0	3	theme	cerevisiae	108:117	arg1	strains					83:89	different strains	73:89	different strains of saccharomyces cerevisiae	73:117	Comparison of structural differences between yeast β-glucan sourced from different strains of saccharomyces cerevisiae and processed using proprietary manufacturing processes.
34352692	4	4	theme	sample	482:487	arg1	pattern					466:472	The linkage pattern	454:472	The linkage pattern of each sample	454:487	The linkage pattern of each sample was measured by the partially methylated alditol acetate method.
34352692	9	5	theme	proprietary	1009:1019	arg1	source					1021:1026	each proprietary source	1004:1026	each proprietary source of yeast β-glucan	1004:1044	Our findings confirm that each proprietary source of yeast β-glucan has a unique purity profile, branching, and linkage patterns that determine the chemical structure and composition.
34352692	8	6	theme	FT-IR	860:864	arg1	spectroscopy					876:887	FT-IR and 1HNMR spectroscopy	860:887	spectroscopy	876:887	The samples were also analyzed by FT-IR and 1HNMR spectroscopy but it was difficult to derive quantitative differences in the samples by these methods.
34352692	0	7	theme	saccharomyces	94:106	arg1	cerevisiae					108:117	saccharomyces cerevisiae	94:117	saccharomyces cerevisiae	94:117	Comparison of structural differences between yeast β-glucan sourced from different strains of saccharomyces cerevisiae and processed using proprietary manufacturing processes.
34352692	9	8	theme	chemical	1126:1133	arg1	structure					1135:1143	the chemical structure	1122:1143	the chemical structure	1122:1143	Our findings confirm that each proprietary source of yeast β-glucan has a unique purity profile, branching, and linkage patterns that determine the chemical structure and composition.
34352692	2	9	theme	β-glucan	303:310	arg1	content					312:318	their β-glucan content	297:318	their β-glucan content	297:318	Samples were assayed for their β-glucan content and the yeast storage carbohydrate, glycogen.
34352692	9	10	theme	unique	1052:1057	arg1	profile					1066:1072	a unique purity profile	1050:1072	a unique purity profile	1050:1072	Our findings confirm that each proprietary source of yeast β-glucan has a unique purity profile, branching, and linkage patterns that determine the chemical structure and composition.
34352692	3	11	theme	β-glucan	370:377	arg1	content					379:385	The β-glucan content	366:385	The β-glucan content ranged from 74% to 86%, the glycogen content	366:430	The β-glucan content ranged from 74% to 86%, the glycogen content varied from 0 to 20%.
34352692	3	12	dep	%	408:408	arg1	to					403:404	to	403:404	to	403:404	The β-glucan content ranged from 74% to 86%, the glycogen content varied from 0 to 20%.
34352692	9	13	theme	yeast	1031:1035	arg1	β-glucan					1037:1044	yeast β-glucan	1031:1044	yeast β-glucan	1031:1044	Our findings confirm that each proprietary source of yeast β-glucan has a unique purity profile, branching, and linkage patterns that determine the chemical structure and composition.
34352692	3	14	theme	%	401:401	arg1	%					408:408	74% to 86%	399:408	74% to 86%	399:408	The β-glucan content ranged from 74% to 86%, the glycogen content varied from 0 to 20%.
34352692	3	14	theme	%	401:401	arg1	content					424:430	the glycogen content	411:430	the glycogen content	411:430	The β-glucan content ranged from 74% to 86%, the glycogen content varied from 0 to 20%.
34352692	4	15	theme	linkage	458:464	arg1	pattern					466:472	The linkage pattern	454:472	The linkage pattern of each sample	454:487	The linkage pattern of each sample was measured by the partially methylated alditol acetate method.
34352692	7	16	theme	length	764:769	arg1	similar					817:823	similar	817:823	similar	817:823	The side length distributions of the samples were shown to be similar.
34352692	7	16	theme	length	764:769	arg1	distributions					771:783	The side length distributions	755:783	The side length distributions of the samples	755:798	The side length distributions of the samples were shown to be similar.
34352692	2	17	theme	storage	334:340	arg1	glycogen					356:363	glycogen	356:363	glycogen	356:363	Samples were assayed for their β-glucan content and the yeast storage carbohydrate, glycogen.
34352692	2	17	theme	storage	334:340	arg1	carbohydrate					342:353	the yeast storage carbohydrate	324:353	the yeast storage carbohydrate	324:353	Samples were assayed for their β-glucan content and the yeast storage carbohydrate, glycogen.
34352692	9	18	theme	β-glucan	1037:1044	arg1	source					1021:1026	each proprietary source	1004:1026	each proprietary source of yeast β-glucan	1004:1044	Our findings confirm that each proprietary source of yeast β-glucan has a unique purity profile, branching, and linkage patterns that determine the chemical structure and composition.
34352692	3	19	theme	glycogen	415:422	arg1	%					408:408	74% to 86%	399:408	74% to 86%	399:408	The β-glucan content ranged from 74% to 86%, the glycogen content varied from 0 to 20%.
34352692	3	19	theme	glycogen	415:422	arg1	content					424:430	the glycogen content	411:430	the glycogen content	411:430	The β-glucan content ranged from 74% to 86%, the glycogen content varied from 0 to 20%.
34352692	4	20	theme	alditol	530:536	arg1	method					546:551	the partially methylated alditol acetate method	505:551	the partially methylated alditol acetate method	505:551	The linkage pattern of each sample was measured by the partially methylated alditol acetate method.
34352692	6	21	theme	ion	735:737	arg1	chromatography					739:752	ion chromatography	735:752	ion chromatography	735:752	The side chain length distribution for each sample was analyzed by an alkaline degradation assay followed by ion chromatography.
34352692	2	22	theme	yeast	328:332	arg1	glycogen					356:363	glycogen	356:363	glycogen	356:363	Samples were assayed for their β-glucan content and the yeast storage carbohydrate, glycogen.
34352692	2	22	theme	yeast	328:332	arg1	carbohydrate					342:353	the yeast storage carbohydrate	324:353	the yeast storage carbohydrate	324:353	Samples were assayed for their β-glucan content and the yeast storage carbohydrate, glycogen.
34352692	6	23	theme	length	641:646	arg1	distribution					648:659	The side chain length distribution	626:659	The side chain length distribution for each sample	626:675	The side chain length distribution for each sample was analyzed by an alkaline degradation assay followed by ion chromatography.
34352692	1	24	theme	structural	203:212	arg1	differences					214:224	structural differences	203:224	structural differences of five commercial samples of yeast β-glucan	203:269	In this study, we explored structural differences of five commercial samples of yeast β-glucan.
34352692	0	25	theme	differences	25:35	arg1	Comparison					0:9	Comparison	0:9	Comparison of structural differences between yeast	0:49	Comparison of structural differences between yeast β-glucan sourced from different strains of saccharomyces cerevisiae and processed using proprietary manufacturing processes.
34352692	4	26	theme	methylated	519:528	arg1	method					546:551	the partially methylated alditol acetate method	505:551	the partially methylated alditol acetate method	505:551	The linkage pattern of each sample was measured by the partially methylated alditol acetate method.
34352692	0	27	theme	proprietary	139:149	arg1	processes					165:173	proprietary manufacturing processes	139:173	proprietary manufacturing processes	139:173	Comparison of structural differences between yeast β-glucan sourced from different strains of saccharomyces cerevisiae and processed using proprietary manufacturing processes.
34352692	6	28	theme	chain	635:639	arg1	distribution					648:659	The side chain length distribution	626:659	The side chain length distribution for each sample	626:675	The side chain length distribution for each sample was analyzed by an alkaline degradation assay followed by ion chromatography.
34352692	0	29	theme	structural	14:23	arg1	differences					25:35	structural differences	14:35	structural differences between yeast	14:49	Comparison of structural differences between yeast β-glucan sourced from different strains of saccharomyces cerevisiae and processed using proprietary manufacturing processes.
34352692	6	30	theme	side	630:633	arg1	distribution					648:659	The side chain length distribution	626:659	The side chain length distribution for each sample	626:675	The side chain length distribution for each sample was analyzed by an alkaline degradation assay followed by ion chromatography.
34352692	7	31	theme	samples	792:798	arg1	similar					817:823	similar	817:823	similar	817:823	The side length distributions of the samples were shown to be similar.
34352692	7	31	theme	samples	792:798	arg1	distributions					771:783	The side length distributions	755:783	The side length distributions of the samples	755:798	The side length distributions of the samples were shown to be similar.
34352692	4	32	theme	acetate	538:544	arg1	method					546:551	the partially methylated alditol acetate method	505:551	the partially methylated alditol acetate method	505:551	The linkage pattern of each sample was measured by the partially methylated alditol acetate method.
34352692	9	33	theme	purity	1059:1064	arg1	profile					1066:1072	a unique purity profile	1050:1072	a unique purity profile	1050:1072	Our findings confirm that each proprietary source of yeast β-glucan has a unique purity profile, branching, and linkage patterns that determine the chemical structure and composition.
34352692	0	34	theme	manufacturing	151:163	arg1	processes					165:173	proprietary manufacturing processes	139:173	proprietary manufacturing processes	139:173	Comparison of structural differences between yeast β-glucan sourced from different strains of saccharomyces cerevisiae and processed using proprietary manufacturing processes.
34352692	0	35	dep	β-glucan	51:58	arg1	sourced					60:66	sourced	60:66	sourced from different strains of saccharomyces cerevisiae	60:117	Comparison of structural differences between yeast β-glucan sourced from different strains of saccharomyces cerevisiae and processed using proprietary manufacturing processes.
34352692	0	35	dep	β-glucan	51:58	arg1	processed					123:131	processed	123:131	processed using proprietary manufacturing processes	123:173	Comparison of structural differences between yeast β-glucan sourced from different strains of saccharomyces cerevisiae and processed using proprietary manufacturing processes.
34352692	8	36	theme	1HNMR	870:874	arg1	spectroscopy					876:887	FT-IR and 1HNMR spectroscopy	860:887	spectroscopy	876:887	The samples were also analyzed by FT-IR and 1HNMR spectroscopy but it was difficult to derive quantitative differences in the samples by these methods.
34352692	1	37	theme	commercial	234:243	arg1	samples					245:251	five commercial samples	229:251	five commercial samples of yeast β-glucan	229:269	In this study, we explored structural differences of five commercial samples of yeast β-glucan.
34352692	7	38	theme	side	759:762	arg1	similar					817:823	similar	817:823	similar	817:823	The side length distributions of the samples were shown to be similar.
34352692	7	38	theme	side	759:762	arg1	distributions					771:783	The side length distributions	755:783	The side length distributions of the samples	755:798	The side length distributions of the samples were shown to be similar.
34352692	5	39	theme	1.9	602:604	arg1	%					613:613	%	613:613	%	613:613	This method showed that the samples varied from 1.9% to 9.2% branching.
34352692	6	40	theme	degradation	705:715	arg1	assay					717:721	an alkaline degradation assay	693:721	an alkaline degradation assay followed by ion chromatography	693:752	The side chain length distribution for each sample was analyzed by an alkaline degradation assay followed by ion chromatography.
34352692	8	41	from	differences	933:943	arg1	samples					952:958	the samples	948:958	the samples	948:958	The samples were also analyzed by FT-IR and 1HNMR spectroscopy but it was difficult to derive quantitative differences in the samples by these methods.
34352692	6	42	theme	alkaline	696:703	arg1	assay					717:721	an alkaline degradation assay	693:721	an alkaline degradation assay followed by ion chromatography	693:752	The side chain length distribution for each sample was analyzed by an alkaline degradation assay followed by ion chromatography.
34352692	1	43	theme	samples	245:251	arg1	differences					214:224	structural differences	203:224	structural differences of five commercial samples of yeast β-glucan	203:269	In this study, we explored structural differences of five commercial samples of yeast β-glucan.
34352692	0	44	theme	different	73:81	arg1	strains					83:89	different strains	73:89	different strains of saccharomyces cerevisiae	73:117	Comparison of structural differences between yeast β-glucan sourced from different strains of saccharomyces cerevisiae and processed using proprietary manufacturing processes.
34352692	3	45	dep	20	449:450	arg1	to					446:447	to	446:447	to	446:447	The β-glucan content ranged from 74% to 86%, the glycogen content varied from 0 to 20%.
34352692	9	46	theme	linkage	1090:1096	arg1	patterns					1098:1105	linkage patterns	1090:1105	linkage patterns	1090:1105	Our findings confirm that each proprietary source of yeast β-glucan has a unique purity profile, branching, and linkage patterns that determine the chemical structure and composition.
34352692	1	47	theme	yeast	256:260	arg1	β-glucan					262:269	yeast β-glucan	256:269	yeast β-glucan	256:269	In this study, we explored structural differences of five commercial samples of yeast β-glucan.
34352692	5	48	dep	%	613:613	arg1	%					605:605	%	605:605	%	605:605	This method showed that the samples varied from 1.9% to 9.2% branching.
34352692	5	48	dep	%	613:613	arg1	to					607:608	to	607:608	to	607:608	This method showed that the samples varied from 1.9% to 9.2% branching.
34352692	1	49	theme	β-glucan	262:269	arg1	samples					245:251	five commercial samples	229:251	five commercial samples of yeast β-glucan	229:269	In this study, we explored structural differences of five commercial samples of yeast β-glucan.
34788748	1	0	theme	cells	267:271	arg1	chondrogenesis					229:242	chondrogenesis	229:242	chondrogenesis	229:242	Hydrogels are of interest in cartilage tissue engineering due to their ability to support the encapsulation and chondrogenesis of mesenchymal stromal cells (MSCs).
34788748	1	0	theme	cells	267:271	arg1	encapsulation					211:223	encapsulation	211:223	encapsulation	211:223	Hydrogels are of interest in cartilage tissue engineering due to their ability to support the encapsulation and chondrogenesis of mesenchymal stromal cells (MSCs).
34788748	7	1	theme	chondrogenic	1452:1463	arg1	expression					1470:1479	chondrogenic gene expression	1452:1479	chondrogenic gene expression	1452:1479	There were minimal differences in chondrogenic gene expression and biochemical content (e.g. DNA, GAG, collagen) between hydrogels alone and composites, whereas the composites increased in compressive modulus to ∼350 kPa after 56 d of culture.
34788748	4	2	theme	soft	847:850	arg1	hydrogels					858:866	soft NorHA hydrogels	847:866	soft NorHA hydrogels	847:866	While soft NorHA hydrogels mature into neocartilage suitable for the repair of articular cartilage, their initial moduli are too low for handling and they do not exhibit the requisite stability needed to withstand the loading environments of articulating joints.
34788748	8	3	theme	contact	1839:1845	arg1	areas					1847:1851	contact areas	1839:1851	contact areas	1839:1851	Lastly, integration of composites with native tissue was assessedex vivo; MSC-laden composites implanted after 28 d of pre-culture exhibited increased integration strengths and contact areas compared to acellular composites.
34788748	4	4	theme	articulating	1083:1094	arg1	joints					1096:1101	articulating joints	1083:1101	articulating joints	1083:1101	While soft NorHA hydrogels mature into neocartilage suitable for the repair of articular cartilage, their initial moduli are too low for handling and they do not exhibit the requisite stability needed to withstand the loading environments of articulating joints.
34788748	5	5	theme	polycaprolactone	1156:1171	arg1	microfibers					1179:1189	polycaprolactone (PCL) microfibers	1156:1189	polycaprolactone (PCL) microfibers produced via melt-electrowriting (MEW)	1156:1228	To address this, we reinforced NorHA hydrogels with polycaprolactone (PCL) microfibers produced via melt-electrowriting (MEW).
34788748	3	6	link	crosslinked	720:730	arg1	hydrogels					758:766	more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels	707:766	more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels	707:766	Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture.
34788748	8	7	theme	acellular	1865:1873	arg1	composites					1875:1884	acellular composites	1865:1884	acellular composites	1865:1884	Lastly, integration of composites with native tissue was assessedex vivo; MSC-laden composites implanted after 28 d of pre-culture exhibited increased integration strengths and contact areas compared to acellular composites.
34788748	8	8	theme	composites	1685:1694	arg1	integration					1670:1680	integration	1670:1680	integration of composites with native tissue	1670:1713	Lastly, integration of composites with native tissue was assessedex vivo; MSC-laden composites implanted after 28 d of pre-culture exhibited increased integration strengths and contact areas compared to acellular composites.
34788748	3	9	dep	kPa	579:581	arg1	softer					568:573	softer	568:573	softer	568:573	Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture.
34788748	4	10	theme	suitable	893:900	arg1	neocartilage					880:891	neocartilage	880:891	neocartilage suitable for the repair of articular cartilage	880:938	While soft NorHA hydrogels mature into neocartilage suitable for the repair of articular cartilage, their initial moduli are too low for handling and they do not exhibit the requisite stability needed to withstand the loading environments of articulating joints.
34788748	3	11	theme	crosslinked	550:560	arg1	hydrogels					628:636	more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels	537:636	more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels	537:636	Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture.
34788748	8	12	theme	native	1701:1706	arg1	tissue					1708:1713	native tissue	1701:1713	native tissue	1701:1713	Lastly, integration of composites with native tissue was assessedex vivo; MSC-laden composites implanted after 28 d of pre-culture exhibited increased integration strengths and contact areas compared to acellular composites.
34788748	6	13	theme	400µm	1285:1289	arg1	spacing					1291:1297	400µm spacing	1285:1297	400µm spacing	1285:1297	Importantly, composites fabricated with MEW meshes of 400µm spacing increased the moduli of soft NorHA hydrogels by ∼50-fold while preserving the chondrogenic potential of the hydrogels.
34788748	9	14	contain	possess	1968:1974	arg1	implants					1954:1961	cell-laden implants	1943:1961	cell-laden implants that possess both initial mechanical integrity and the ability to support neocartilage formation and integration for cartilage repair	1943:2095	This approach has great potential towards the design of cell-laden implants that possess both initial mechanical integrity and the ability to support neocartilage formation and integration for cartilage repair.
34788748	9	14	contain	possess	1968:1974	arg2	ability					2018:2024	the ability	2014:2024	the ability to support neocartilage formation and integration for cartilage repair	2014:2095	This approach has great potential towards the design of cell-laden implants that possess both initial mechanical integrity and the ability to support neocartilage formation and integration for cartilage repair.
34788748	9	14	contain	possess	1968:1974	arg2	integrity					2000:2008	initial mechanical integrity	1981:2008	initial mechanical integrity	1981:2008	This approach has great potential towards the design of cell-laden implants that possess both initial mechanical integrity and the ability to support neocartilage formation and integration for cartilage repair.
34788748	9	15	theme	implants	1954:1961	arg1	design					1933:1938	the design	1929:1938	the design of cell-laden implants that possess both initial mechanical integrity and the ability to support neocartilage formation and integration for cartilage repair	1929:2095	This approach has great potential towards the design of cell-laden implants that possess both initial mechanical integrity and the ability to support neocartilage formation and integration for cartilage repair.
34788748	3	16	theme	acid	615:618	arg1	hydrogels					628:636	more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels	537:636	more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels	537:636	Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture.
34788748	8	17	theme	MSC-laden	1736:1744	arg1	composites					1746:1755	MSC-laden composites	1736:1755	MSC-laden composites implanted after 28 d of pre-culture	1736:1791	Lastly, integration of composites with native tissue was assessedex vivo; MSC-laden composites implanted after 28 d of pre-culture exhibited increased integration strengths and contact areas compared to acellular composites.
34788748	3	18	theme	norbornene-modified	584:602	arg1	hydrogels					628:636	more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels	537:636	more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels	537:636	Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture.
34788748	3	19	dep	crosslinked	550:560	arg1	kPa					579:581	∼2 kPa	576:581	∼2 kPa	576:581	Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture.
34788748	8	20	with	integration	1670:1680	arg1	tissue					1708:1713	native tissue	1701:1713	native tissue	1701:1713	Lastly, integration of composites with native tissue was assessedex vivo; MSC-laden composites implanted after 28 d of pre-culture exhibited increased integration strengths and contact areas compared to acellular composites.
34788748	9	21	theme	initial	1981:1987	arg1	integrity					2000:2008	initial mechanical integrity	1981:2008	initial mechanical integrity	1981:2008	This approach has great potential towards the design of cell-laden implants that possess both initial mechanical integrity and the ability to support neocartilage formation and integration for cartilage repair.
34788748	3	22	theme	compressive	798:808	arg1	modulus					810:816	compressive modulus	798:816	compressive modulus after 56 d of culture	798:838	Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture.
34788748	0	23	theme	MEW	81:83	arg1	scaffolds					85:93	MEW scaffolds	81:93	MEW scaffolds for cartilage repair	81:114	Fabrication of MSC-laden composites of hyaluronic acid hydrogels reinforced with MEW scaffolds for cartilage repair.
34788748	3	24	dep	stiffer	738:744	arg1	i.e.					733:736	i.e.	733:736	i.e.	733:736	Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture.
34788748	3	25	theme	culture	832:838	arg1	d					827:827	56 d	824:827	56 d of culture	824:838	Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture.
34788748	6	26	theme	soft	1323:1326	arg1	hydrogels					1334:1342	soft NorHA hydrogels	1323:1342	soft NorHA hydrogels	1323:1342	Importantly, composites fabricated with MEW meshes of 400µm spacing increased the moduli of soft NorHA hydrogels by ∼50-fold while preserving the chondrogenic potential of the hydrogels.
34788748	4	27	theme	cartilage	930:938	arg1	repair					910:915	the repair	906:915	the repair of articular cartilage	906:938	While soft NorHA hydrogels mature into neocartilage suitable for the repair of articular cartilage, their initial moduli are too low for handling and they do not exhibit the requisite stability needed to withstand the loading environments of articulating joints.
34788748	2	28	theme	nascent	375:381	arg1	distribution					390:401	nascent matrix distribution	375:401	nascent matrix distribution	375:401	However, features such as hydrogel crosslink density, which can influence nutrient transport, nascent matrix distribution, and the stability of constructs during and after implantation must be considered in hydrogel design.
34788748	6	29	theme	hydrogels	1407:1415	arg1	potential					1390:1398	the chondrogenic potential	1373:1398	the chondrogenic potential of the hydrogels	1373:1415	Importantly, composites fabricated with MEW meshes of 400µm spacing increased the moduli of soft NorHA hydrogels by ∼50-fold while preserving the chondrogenic potential of the hydrogels.
34788748	8	30	theme	integration	1813:1823	arg1	strengths					1825:1833	increased integration strengths	1803:1833	increased integration strengths	1803:1833	Lastly, integration of composites with native tissue was assessedex vivo; MSC-laden composites implanted after 28 d of pre-culture exhibited increased integration strengths and contact areas compared to acellular composites.
34788748	2	31	theme	constructs	425:434	arg1	distribution					390:401	nascent matrix distribution	375:401	nascent matrix distribution	375:401	However, features such as hydrogel crosslink density, which can influence nutrient transport, nascent matrix distribution, and the stability of constructs during and after implantation must be considered in hydrogel design.
34788748	2	31	theme	constructs	425:434	arg1	stability					412:420	the stability	408:420	the stability of constructs	408:434	However, features such as hydrogel crosslink density, which can influence nutrient transport, nascent matrix distribution, and the stability of constructs during and after implantation must be considered in hydrogel design.
34788748	2	31	theme	constructs	425:434	arg1	transport					364:372	nutrient transport	355:372	nutrient transport	355:372	However, features such as hydrogel crosslink density, which can influence nutrient transport, nascent matrix distribution, and the stability of constructs during and after implantation must be considered in hydrogel design.
34788748	3	32	from	increase	786:793	arg1	modulus					810:816	compressive modulus	798:816	compressive modulus after 56 d of culture	798:838	Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture.
34788748	9	33	theme	neocartilage	2037:2048	arg1	formation					2050:2058	neocartilage formation	2037:2058	neocartilage formation	2037:2058	This approach has great potential towards the design of cell-laden implants that possess both initial mechanical integrity and the ability to support neocartilage formation and integration for cartilage repair.
34788748	0	34	theme	MSC-laden	15:23	arg1	composites					25:34	MSC-laden composites	15:34	MSC-laden composites of hyaluronic acid	15:53	Fabrication of MSC-laden composites of hyaluronic acid hydrogels reinforced with MEW scaffolds for cartilage repair.
34788748	0	34	theme	MSC-laden	15:23	arg1	acid					50:53	hyaluronic acid	39:53	hyaluronic acid	39:53	Fabrication of MSC-laden composites of hyaluronic acid hydrogels reinforced with MEW scaffolds for cartilage repair.
34788748	8	35	theme	pre-culture	1781:1791	arg1	d					1776:1776	28 d	1773:1776	28 d of pre-culture	1773:1791	Lastly, integration of composites with native tissue was assessedex vivo; MSC-laden composites implanted after 28 d of pre-culture exhibited increased integration strengths and contact areas compared to acellular composites.
34788748	6	36	theme	chondrogenic	1377:1388	arg1	potential					1390:1398	the chondrogenic potential	1373:1398	the chondrogenic potential of the hydrogels	1373:1415	Importantly, composites fabricated with MEW meshes of 400µm spacing increased the moduli of soft NorHA hydrogels by ∼50-fold while preserving the chondrogenic potential of the hydrogels.
34788748	2	37	theme	hydrogel	488:495	arg1	design					497:502	hydrogel design	488:502	hydrogel design	488:502	However, features such as hydrogel crosslink density, which can influence nutrient transport, nascent matrix distribution, and the stability of constructs during and after implantation must be considered in hydrogel design.
34788748	0	38	theme	acid	50:53	arg1	composites					25:34	MSC-laden composites	15:34	MSC-laden composites of hyaluronic acid	15:53	Fabrication of MSC-laden composites of hyaluronic acid hydrogels reinforced with MEW scaffolds for cartilage repair.
34788748	0	38	theme	acid	50:53	arg1	acid					50:53	hyaluronic acid	39:53	hyaluronic acid	39:53	Fabrication of MSC-laden composites of hyaluronic acid hydrogels reinforced with MEW scaffolds for cartilage repair.
34788748	1	39	theme	cartilage	146:154	arg1	engineering					163:173	cartilage tissue engineering	146:173	cartilage tissue engineering due to their ability to support the encapsulation and chondrogenesis of mesenchymal stromal cells (MSCs)	146:278	Hydrogels are of interest in cartilage tissue engineering due to their ability to support the encapsulation and chondrogenesis of mesenchymal stromal cells (MSCs).
34788748	3	40	theme	enhanced	646:653	arg1	formation					665:673	enhanced cartilage formation	646:673	enhanced cartilage formation	646:673	Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture.
34788748	7	41	theme	gene	1465:1468	arg1	expression					1470:1479	chondrogenic gene expression	1452:1479	chondrogenic gene expression	1452:1479	There were minimal differences in chondrogenic gene expression and biochemical content (e.g. DNA, GAG, collagen) between hydrogels alone and composites, whereas the composites increased in compressive modulus to ∼350 kPa after 56 d of culture.
34788748	1	42	theme	stromal	259:265	arg1	cells					267:271	mesenchymal stromal cells	247:271	mesenchymal stromal cells (MSCs)	247:278	Hydrogels are of interest in cartilage tissue engineering due to their ability to support the encapsulation and chondrogenesis of mesenchymal stromal cells (MSCs).
34788748	1	42	theme	stromal	259:265	arg1	MSCs					274:277	MSCs	274:277	MSCs	274:277	Hydrogels are of interest in cartilage tissue engineering due to their ability to support the encapsulation and chondrogenesis of mesenchymal stromal cells (MSCs).
34788748	4	43	theme	requisite	1015:1023	arg1	stability					1025:1033	the requisite stability	1011:1033	the requisite stability needed to withstand the loading environments of articulating joints	1011:1101	While soft NorHA hydrogels mature into neocartilage suitable for the repair of articular cartilage, their initial moduli are too low for handling and they do not exhibit the requisite stability needed to withstand the loading environments of articulating joints.
34788748	7	44	theme	minimal	1429:1435	arg1	differences					1437:1447	minimal differences	1429:1447	minimal differences in chondrogenic gene expression and biochemical content (e.g. DNA, GAG, collagen) between hydrogels alone and composites	1429:1568	There were minimal differences in chondrogenic gene expression and biochemical content (e.g. DNA, GAG, collagen) between hydrogels alone and composites, whereas the composites increased in compressive modulus to ∼350 kPa after 56 d of culture.
34788748	4	45	theme	joints	1096:1101	arg1	environments					1067:1078	the loading environments	1055:1078	the loading environments of articulating joints	1055:1101	While soft NorHA hydrogels mature into neocartilage suitable for the repair of articular cartilage, their initial moduli are too low for handling and they do not exhibit the requisite stability needed to withstand the loading environments of articulating joints.
34788748	3	46	theme	crosslinked	720:730	arg1	hydrogels					758:766	more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels	707:766	more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels	707:766	Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture.
34788748	3	47	dep	softer	568:573	arg1	i.e.					563:566	i.e.	563:566	i.e.	563:566	Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture.
34788748	6	48	theme	hydrogels	1334:1342	arg1	moduli					1313:1318	the moduli	1309:1318	the moduli of soft NorHA hydrogels by ∼50-fold	1309:1354	Importantly, composites fabricated with MEW meshes of 400µm spacing increased the moduli of soft NorHA hydrogels by ∼50-fold while preserving the chondrogenic potential of the hydrogels.
34788748	2	49	theme	hydrogel	307:314	arg1	density					326:332	hydrogel crosslink density	307:332	hydrogel crosslink density	307:332	However, features such as hydrogel crosslink density, which can influence nutrient transport, nascent matrix distribution, and the stability of constructs during and after implantation must be considered in hydrogel design.
34788748	7	50	theme	compressive	1607:1617	arg1	modulus					1619:1625	compressive modulus	1607:1625	compressive modulus	1607:1625	There were minimal differences in chondrogenic gene expression and biochemical content (e.g. DNA, GAG, collagen) between hydrogels alone and composites, whereas the composites increased in compressive modulus to ∼350 kPa after 56 d of culture.
34788748	3	51	theme	cartilage	655:663	arg1	formation					665:673	enhanced cartilage formation	646:673	enhanced cartilage formation	646:673	Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture.
34788748	9	52	theme	great	1905:1909	arg1	potential					1911:1919	great potential	1905:1919	great potential towards the design of cell-laden implants that possess both initial mechanical integrity and the ability to support neocartilage formation and integration for cartilage repair	1905:2095	This approach has great potential towards the design of cell-laden implants that possess both initial mechanical integrity and the ability to support neocartilage formation and integration for cartilage repair.
34788748	7	53	from	differences	1437:1447	arg1	content					1497:1503	biochemical content	1485:1503	biochemical content (e.g. DNA, GAG, collagen)	1485:1529	There were minimal differences in chondrogenic gene expression and biochemical content (e.g. DNA, GAG, collagen) between hydrogels alone and composites, whereas the composites increased in compressive modulus to ∼350 kPa after 56 d of culture.
34788748	7	53	from	differences	1437:1447	arg1	expression					1470:1479	chondrogenic gene expression	1452:1479	chondrogenic gene expression	1452:1479	There were minimal differences in chondrogenic gene expression and biochemical content (e.g. DNA, GAG, collagen) between hydrogels alone and composites, whereas the composites increased in compressive modulus to ∼350 kPa after 56 d of culture.
34788748	4	54	theme	loading	1059:1065	arg1	environments					1067:1078	the loading environments	1055:1078	the loading environments of articulating joints	1055:1101	While soft NorHA hydrogels mature into neocartilage suitable for the repair of articular cartilage, their initial moduli are too low for handling and they do not exhibit the requisite stability needed to withstand the loading environments of articulating joints.
34788748	6	55	theme	spacing	1291:1297	arg1	meshes					1275:1280	MEW meshes	1271:1280	MEW meshes of 400µm spacing	1271:1297	Importantly, composites fabricated with MEW meshes of 400µm spacing increased the moduli of soft NorHA hydrogels by ∼50-fold while preserving the chondrogenic potential of the hydrogels.
34788748	6	56	theme	NorHA	1328:1332	arg1	hydrogels					1334:1342	soft NorHA hydrogels	1323:1342	soft NorHA hydrogels	1323:1342	Importantly, composites fabricated with MEW meshes of 400µm spacing increased the moduli of soft NorHA hydrogels by ∼50-fold while preserving the chondrogenic potential of the hydrogels.
34788748	4	57	theme	NorHA	852:856	arg1	hydrogels					858:866	soft NorHA hydrogels	847:866	soft NorHA hydrogels	847:866	While soft NorHA hydrogels mature into neocartilage suitable for the repair of articular cartilage, their initial moduli are too low for handling and they do not exhibit the requisite stability needed to withstand the loading environments of articulating joints.
34788748	9	58	theme	cell-laden	1943:1952	arg1	implants					1954:1961	cell-laden implants	1943:1961	cell-laden implants that possess both initial mechanical integrity and the ability to support neocartilage formation and integration for cartilage repair	1943:2095	This approach has great potential towards the design of cell-laden implants that possess both initial mechanical integrity and the ability to support neocartilage formation and integration for cartilage repair.
34788748	6	59	theme	MEW	1271:1273	arg1	meshes					1275:1280	MEW meshes	1271:1280	MEW meshes of 400µm spacing	1271:1297	Importantly, composites fabricated with MEW meshes of 400µm spacing increased the moduli of soft NorHA hydrogels by ∼50-fold while preserving the chondrogenic potential of the hydrogels.
34788748	3	60	theme	hyaluronic	604:613	arg1	NorHA					621:625	NorHA	621:625	NorHA	621:625	Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture.
34788748	3	60	theme	hyaluronic	604:613	arg1	acid					615:618	hyaluronic acid	604:618	more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels	537:636	Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture.
34788748	3	61	theme	>100-fold	776:784	arg1	increase					786:793	a >100-fold increase	774:793	a >100-fold increase in compressive modulus after 56 d of culture	774:838	Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture.
34788748	3	62	link	crosslinked	550:560	arg1	hydrogels					628:636	more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels	537:636	more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels	537:636	Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture.
34788748	1	63	theme	tissue	156:161	arg1	engineering					163:173	cartilage tissue engineering	146:173	cartilage tissue engineering due to their ability to support the encapsulation and chondrogenesis of mesenchymal stromal cells (MSCs)	146:278	Hydrogels are of interest in cartilage tissue engineering due to their ability to support the encapsulation and chondrogenesis of mesenchymal stromal cells (MSCs).
34788748	4	64	theme	initial	947:953	arg1	moduli					955:960	their initial moduli	941:960	their initial moduli	941:960	While soft NorHA hydrogels mature into neocartilage suitable for the repair of articular cartilage, their initial moduli are too low for handling and they do not exhibit the requisite stability needed to withstand the loading environments of articulating joints.
34788748	9	65	theme	mechanical	1989:1998	arg1	integrity					2000:2008	initial mechanical integrity	1981:2008	initial mechanical integrity	1981:2008	This approach has great potential towards the design of cell-laden implants that possess both initial mechanical integrity and the ability to support neocartilage formation and integration for cartilage repair.
34788748	0	66	theme	cartilage	99:107	arg1	repair					109:114	cartilage repair	99:114	cartilage repair	99:114	Fabrication of MSC-laden composites of hyaluronic acid hydrogels reinforced with MEW scaffolds for cartilage repair.
34788748	3	67	dep	kPa	753:755	arg1	stiffer					738:744	stiffer	738:744	stiffer	738:744	Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture.
34788748	7	68	theme	culture	1653:1659	arg1	d					1648:1648	56 d	1645:1648	56 d of culture	1645:1659	There were minimal differences in chondrogenic gene expression and biochemical content (e.g. DNA, GAG, collagen) between hydrogels alone and composites, whereas the composites increased in compressive modulus to ∼350 kPa after 56 d of culture.
34788748	4	69	theme	articular	920:928	arg1	cartilage					930:938	articular cartilage	920:938	articular cartilage	920:938	While soft NorHA hydrogels mature into neocartilage suitable for the repair of articular cartilage, their initial moduli are too low for handling and they do not exhibit the requisite stability needed to withstand the loading environments of articulating joints.
34788748	2	70	theme	matrix	383:388	arg1	distribution					390:401	nascent matrix distribution	375:401	nascent matrix distribution	375:401	However, features such as hydrogel crosslink density, which can influence nutrient transport, nascent matrix distribution, and the stability of constructs during and after implantation must be considered in hydrogel design.
34788748	1	71	from	interest	134:141	arg1	engineering					163:173	cartilage tissue engineering	146:173	cartilage tissue engineering due to their ability to support the encapsulation and chondrogenesis of mesenchymal stromal cells (MSCs)	146:278	Hydrogels are of interest in cartilage tissue engineering due to their ability to support the encapsulation and chondrogenesis of mesenchymal stromal cells (MSCs).
34788748	0	72	theme	composites	25:34	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of MSC-laden composites of hyaluronic acid	0:53	Fabrication of MSC-laden composites of hyaluronic acid hydrogels reinforced with MEW scaffolds for cartilage repair.
34788748	5	73	theme	NorHA	1135:1139	arg1	hydrogels					1141:1149	NorHA hydrogels	1135:1149	NorHA hydrogels	1135:1149	To address this, we reinforced NorHA hydrogels with polycaprolactone (PCL) microfibers produced via melt-electrowriting (MEW).
34788748	8	74	theme	increased	1803:1811	arg1	strengths					1825:1833	increased integration strengths	1803:1833	increased integration strengths	1803:1833	Lastly, integration of composites with native tissue was assessedex vivo; MSC-laden composites implanted after 28 d of pre-culture exhibited increased integration strengths and contact areas compared to acellular composites.
34788748	0	75	theme	hyaluronic	39:48	arg1	acid					50:53	hyaluronic acid	39:53	hyaluronic acid	39:53	Fabrication of MSC-laden composites of hyaluronic acid hydrogels reinforced with MEW scaffolds for cartilage repair.
34788748	0	76	dep	hydrogels	55:63	arg1	reinforced					65:74	reinforced	65:74	hydrogels reinforced with MEW scaffolds for cartilage repair	55:114	Fabrication of MSC-laden composites of hyaluronic acid hydrogels reinforced with MEW scaffolds for cartilage repair.
34788748	9	77	theme	cartilage	2080:2088	arg1	repair					2090:2095	cartilage repair	2080:2095	cartilage repair	2080:2095	This approach has great potential towards the design of cell-laden implants that possess both initial mechanical integrity and the ability to support neocartilage formation and integration for cartilage repair.
34788748	3	78	dep	crosslinked	720:730	arg1	kPa					753:755	∼6-60 kPa	747:755	∼6-60 kPa	747:755	Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture.
34788748	1	79	dep	encapsulation	211:223	arg1	the					207:209	the	207:209	the	207:209	Hydrogels are of interest in cartilage tissue engineering due to their ability to support the encapsulation and chondrogenesis of mesenchymal stromal cells (MSCs).
34788748	2	80	theme	crosslink	316:324	arg1	density					326:332	hydrogel crosslink density	307:332	hydrogel crosslink density	307:332	However, features such as hydrogel crosslink density, which can influence nutrient transport, nascent matrix distribution, and the stability of constructs during and after implantation must be considered in hydrogel design.
34788748	7	81	dep	DNA	1511:1513	arg1	e.g.					1506:1509	e.g.	1506:1509	e.g.	1506:1509	There were minimal differences in chondrogenic gene expression and biochemical content (e.g. DNA, GAG, collagen) between hydrogels alone and composites, whereas the composites increased in compressive modulus to ∼350 kPa after 56 d of culture.
34788748	7	82	theme	biochemical	1485:1495	arg1	content					1497:1503	biochemical content	1485:1503	biochemical content (e.g. DNA, GAG, collagen)	1485:1529	There were minimal differences in chondrogenic gene expression and biochemical content (e.g. DNA, GAG, collagen) between hydrogels alone and composites, whereas the composites increased in compressive modulus to ∼350 kPa after 56 d of culture.
34788748	9	83	contain	has	1901:1903	arg1	approach					1892:1899	This approach	1887:1899	This approach	1887:1899	This approach has great potential towards the design of cell-laden implants that possess both initial mechanical integrity and the ability to support neocartilage formation and integration for cartilage repair.
34788748	9	83	contain	has	1901:1903	arg2	potential					1911:1919	great potential	1905:1919	great potential towards the design of cell-laden implants that possess both initial mechanical integrity and the ability to support neocartilage formation and integration for cartilage repair	1905:2095	This approach has great potential towards the design of cell-laden implants that possess both initial mechanical integrity and the ability to support neocartilage formation and integration for cartilage repair.
34788748	2	84	theme	nutrient	355:362	arg1	transport					364:372	nutrient transport	355:372	nutrient transport	355:372	However, features such as hydrogel crosslink density, which can influence nutrient transport, nascent matrix distribution, and the stability of constructs during and after implantation must be considered in hydrogel design.
34788748	1	85	theme	mesenchymal	247:257	arg1	cells					267:271	mesenchymal stromal cells	247:271	mesenchymal stromal cells (MSCs)	247:278	Hydrogels are of interest in cartilage tissue engineering due to their ability to support the encapsulation and chondrogenesis of mesenchymal stromal cells (MSCs).
34788748	1	85	theme	mesenchymal	247:257	arg1	MSCs					274:277	MSCs	274:277	MSCs	274:277	Hydrogels are of interest in cartilage tissue engineering due to their ability to support the encapsulation and chondrogenesis of mesenchymal stromal cells (MSCs).
34680064	0	0	theme	Drug	78:81	arg1	Delivery					83:90	Ocular Drug Delivery	71:90	Ocular Drug Delivery	71:90	Insight into the Lubrication and Adhesion Properties of Hyaluronan for Ocular Drug Delivery.
34680064	10	1	theme	friction	1106:1113	arg1	coefficient					1115:1125	the friction coefficient	1102:1125	the friction coefficient	1102:1125	The presence of trehalose increases the friction coefficient.
34680064	1	2	theme	eye	128:130	arg1	drops					132:136	eye drops	128:136	eye drops	128:136	Hyaluronan (HA) is widely used for eye drops as lubricant to counteract dry eye disease.
34680064	13	3	dep	specific	1510:1517	arg1	needs					1519:1523	needs	1519:1523	needs	1519:1523	The results here presented also provide valuable information for the design of highly performing HA-formulations addressing specific needs before preclinic.
34680064	0	4	theme	Ocular	71:76	arg1	Delivery					83:90	Ocular Drug Delivery	71:90	Ocular Drug Delivery	71:90	Insight into the Lubrication and Adhesion Properties of Hyaluronan for Ocular Drug Delivery.
34680064	13	5	theme	valuable	1426:1433	arg1	information					1435:1445	valuable information	1426:1445	valuable information for the design of highly performing HA-formulations addressing specific needs before preclinic	1426:1540	The results here presented also provide valuable information for the design of highly performing HA-formulations addressing specific needs before preclinic.
34680064	13	6	theme	HA-formulations	1483:1497	arg1	design					1455:1460	the design	1451:1460	the design of highly performing HA-formulations addressing specific needs before preclinic	1451:1540	The results here presented also provide valuable information for the design of highly performing HA-formulations addressing specific needs before preclinic.
34680064	11	7	theme	lower	1175:1179	arg1	coefficient					1190:1200	significantly lower friction coefficient	1161:1200	significantly lower friction coefficient	1161:1200	Medium molecular weight HA shows significantly lower friction coefficient than high molecular weight HA.
34680064	9	8	theme	in-vitro	1033:1040	arg1	model					1059:1063	an in-vitro cell-desiccation model	1030:1063	an in-vitro cell-desiccation model	1030:1063	The incorporation of trehalose enhances the protection of human keratinocytes (HaCaT) cells, as demonstrated in an in-vitro cell-desiccation model.
34680064	3	9	from	lubrication	319:329	arg1	portion					269:275	a large portion	261:275	a large portion of the current literature on friction and lubrication	261:329	However, a large portion of the current literature on friction and lubrication addresses articular (joint) cartilage.
34680064	11	10	theme	friction	1181:1188	arg1	coefficient					1190:1200	significantly lower friction coefficient	1161:1200	significantly lower friction coefficient	1161:1200	Medium molecular weight HA shows significantly lower friction coefficient than high molecular weight HA.
34680064	6	11	theme	friction	712:719	arg1	coefficient					721:731	the friction coefficient	708:731	the friction coefficient	708:731	The tribological investigation reveals that amphiphilic HA-C12 decreases the friction coefficient.
34680064	3	12	theme	literature	292:301	arg1	portion					269:275	a large portion	261:275	a large portion of the current literature on friction and lubrication	261:329	However, a large portion of the current literature on friction and lubrication addresses articular (joint) cartilage.
34680064	9	13	theme	cell-desiccation	1042:1057	arg1	model					1059:1063	an in-vitro cell-desiccation model	1030:1063	an in-vitro cell-desiccation model	1030:1063	The incorporation of trehalose enhances the protection of human keratinocytes (HaCaT) cells, as demonstrated in an in-vitro cell-desiccation model.
34680064	12	14	theme	eye	1314:1316	arg1	drops					1318:1322	eye drops	1314:1322	eye drops	1314:1322	This research represents a first, wide array of features of diverse HA forms for eye drops contributing to increase the knowledge of these preparations.
34680064	9	15	theme	HaCaT	997:1001	arg1	cells					1004:1008	human keratinocytes (HaCaT) cells	976:1008	human keratinocytes (HaCaT) cells	976:1008	The incorporation of trehalose enhances the protection of human keratinocytes (HaCaT) cells, as demonstrated in an in-vitro cell-desiccation model.
34680064	9	16	theme	human	976:980	arg1	cells					1004:1008	human keratinocytes (HaCaT) cells	976:1008	human keratinocytes (HaCaT) cells	976:1008	The incorporation of trehalose enhances the protection of human keratinocytes (HaCaT) cells, as demonstrated in an in-vitro cell-desiccation model.
34680064	1	17	used	used	119:122	arg2	HA					105:106	HA	105:106	HA	105:106	Hyaluronan (HA) is widely used for eye drops as lubricant to counteract dry eye disease.
34680064	1	17	used	used	119:122	arg2	Hyaluronan					93:102	Hyaluronan	93:102	Hyaluronan (HA)	93:107	Hyaluronan (HA) is widely used for eye drops as lubricant to counteract dry eye disease.
34680064	1	17	used	used	119:122	arg2	lubricant					141:149	lubricant	141:149	lubricant	141:149	Hyaluronan (HA) is widely used for eye drops as lubricant to counteract dry eye disease.
34680064	8	18	theme	residence	876:884	arg1	time					886:889	a prolonged residence time	864:889	a prolonged residence time	864:889	Thus, it is predicted a prolonged residence time on the surface of the eye.
34680064	12	19	theme	features	1281:1288	arg1	array					1272:1276	a first, wide array	1258:1276	array	1272:1276	This research represents a first, wide array of features of diverse HA forms for eye drops contributing to increase the knowledge of these preparations.
34680064	3	20	theme	articular	341:349	arg1	cartilage					359:367	articular (joint) cartilage	341:367	articular (joint) cartilage	341:367	However, a large portion of the current literature on friction and lubrication addresses articular (joint) cartilage.
34680064	4	21	theme	derivatized	424:434	arg1	forms					436:440	its derivatized forms	420:440	its derivatized forms	420:440	Therefore, eye drops compositions based on HA and its derivatized forms are extensively characterized providing data on the tribological and mucoadhesive properties.
34680064	5	22	theme	eye	611:613	arg1	formulations					621:632	eye drops formulations	611:632	eye drops formulations	611:632	The physiochemical properties are investigated in buffers used commonly in eye drops formulations.
34680064	5	23	theme	physiochemical	540:553	arg1	properties					555:564	The physiochemical properties	536:564	The physiochemical properties	536:564	The physiochemical properties are investigated in buffers used commonly in eye drops formulations.
34680064	11	24	theme	high	1207:1210	arg1	weight					1222:1227	high molecular weight	1207:1227	high molecular weight HA	1207:1230	Medium molecular weight HA shows significantly lower friction coefficient than high molecular weight HA.
34680064	8	25	theme	prolonged	866:874	arg1	time					886:889	a prolonged residence time	864:889	a prolonged residence time	864:889	Thus, it is predicted a prolonged residence time on the surface of the eye.
34680064	12	26	theme	HA	1301:1302	arg1	forms					1304:1308	diverse HA forms	1293:1308	diverse HA forms	1293:1308	This research represents a first, wide array of features of diverse HA forms for eye drops contributing to increase the knowledge of these preparations.
34680064	4	27	theme	mucoadhesive	511:522	arg1	properties					524:533	the tribological and mucoadhesive properties	490:533	the tribological and mucoadhesive properties	490:533	Therefore, eye drops compositions based on HA and its derivatized forms are extensively characterized providing data on the tribological and mucoadhesive properties.
34680064	5	28	theme	drops	615:619	arg1	formulations					621:632	eye drops formulations	611:632	eye drops formulations	611:632	The physiochemical properties are investigated in buffers used commonly in eye drops formulations.
34680064	11	29	theme	molecular	1212:1220	arg1	weight					1222:1227	high molecular weight	1207:1227	high molecular weight HA	1207:1230	Medium molecular weight HA shows significantly lower friction coefficient than high molecular weight HA.
34680064	6	30	theme	amphiphilic	679:689	arg1	HA-C12					691:696	amphiphilic HA-C12	679:696	amphiphilic HA-C12	679:696	The tribological investigation reveals that amphiphilic HA-C12 decreases the friction coefficient.
34680064	12	31	theme	diverse	1293:1299	arg1	forms					1304:1308	diverse HA forms	1293:1308	diverse HA forms	1293:1308	This research represents a first, wide array of features of diverse HA forms for eye drops contributing to increase the knowledge of these preparations.
34680064	1	32	dep	lubricant	141:149	arg1	counteract					154:163	counteract	154:163	to counteract dry eye disease	151:179	Hyaluronan (HA) is widely used for eye drops as lubricant to counteract dry eye disease.
34680064	12	33	theme	preparations	1372:1383	arg1	knowledge					1353:1361	the knowledge	1349:1361	the knowledge of these preparations	1349:1383	This research represents a first, wide array of features of diverse HA forms for eye drops contributing to increase the knowledge of these preparations.
34680064	1	34	theme	dry	165:167	arg1	disease					173:179	dry eye disease	165:179	dry eye disease	165:179	Hyaluronan (HA) is widely used for eye drops as lubricant to counteract dry eye disease.
34680064	7	35	theme	HAC12	787:791	arg1	combination					756:766	the combination	752:766	the combination of trehalose/HA or HAC12	752:791	At the same time, the combination of trehalose/HA or HAC12 enhances up to eighty-fold the mucoadhesiveness.
34680064	3	36	from	literature	292:301	arg1	lubrication					319:329	lubrication	319:329	lubrication	319:329	However, a large portion of the current literature on friction and lubrication addresses articular (joint) cartilage.
34680064	3	36	from	literature	292:301	arg1	friction					306:313	friction	306:313	friction	306:313	However, a large portion of the current literature on friction and lubrication addresses articular (joint) cartilage.
34680064	1	37	theme	eye	169:171	arg1	disease					173:179	dry eye disease	165:179	dry eye disease	165:179	Hyaluronan (HA) is widely used for eye drops as lubricant to counteract dry eye disease.
34680064	0	38	theme	Adhesion	33:40	arg1	Properties					42:51	Adhesion Properties	33:51	Adhesion Properties	33:51	Insight into the Lubrication and Adhesion Properties of Hyaluronan for Ocular Drug Delivery.
34680064	11	39	theme	weight	1222:1227	arg1	HA					1229:1230	high molecular weight HA	1207:1230	high molecular weight HA	1207:1230	Medium molecular weight HA shows significantly lower friction coefficient than high molecular weight HA.
34680064	2	40	theme	molecular	195:203	arg1	HA					212:213	High and low molecular weight HA	182:213	High and low molecular weight HA	182:213	High and low molecular weight HA are currently used in ophthalmology.
34680064	13	41	theme	performing	1472:1481	arg1	HA-formulations					1483:1497	highly performing HA-formulations	1465:1497	highly performing HA-formulations addressing specific needs before preclinic	1465:1540	The results here presented also provide valuable information for the design of highly performing HA-formulations addressing specific needs before preclinic.
34680064	9	42	theme	keratinocytes	982:994	arg1	cells					1004:1008	human keratinocytes (HaCaT) cells	976:1008	human keratinocytes (HaCaT) cells	976:1008	The incorporation of trehalose enhances the protection of human keratinocytes (HaCaT) cells, as demonstrated in an in-vitro cell-desiccation model.
34680064	7	43	theme	trehalose/HA	771:782	arg1	combination					756:766	the combination	752:766	the combination of trehalose/HA or HAC12	752:791	At the same time, the combination of trehalose/HA or HAC12 enhances up to eighty-fold the mucoadhesiveness.
34680064	3	44	from	friction	306:313	arg1	portion					269:275	a large portion	261:275	a large portion of the current literature on friction and lubrication	261:329	However, a large portion of the current literature on friction and lubrication addresses articular (joint) cartilage.
34680064	2	45	theme	low	191:193	arg1	HA					212:213	High and low molecular weight HA	182:213	High and low molecular weight HA	182:213	High and low molecular weight HA are currently used in ophthalmology.
34680064	9	46	theme	trehalose	939:947	arg1	incorporation					922:934	The incorporation	918:934	The incorporation of trehalose	918:947	The incorporation of trehalose enhances the protection of human keratinocytes (HaCaT) cells, as demonstrated in an in-vitro cell-desiccation model.
34680064	3	47	theme	joint	352:356	arg1	cartilage					359:367	articular (joint) cartilage	341:367	articular (joint) cartilage	341:367	However, a large portion of the current literature on friction and lubrication addresses articular (joint) cartilage.
34680064	0	48	theme	Hyaluronan	56:65	arg1	Lubrication					17:27	Lubrication	17:27	Lubrication	17:27	Insight into the Lubrication and Adhesion Properties of Hyaluronan for Ocular Drug Delivery.
34680064	0	48	theme	Hyaluronan	56:65	arg1	Properties					42:51	Adhesion Properties	33:51	Adhesion Properties	33:51	Insight into the Lubrication and Adhesion Properties of Hyaluronan for Ocular Drug Delivery.
34680064	4	49	from	data	482:485	arg1	properties					524:533	the tribological and mucoadhesive properties	490:533	the tribological and mucoadhesive properties	490:533	Therefore, eye drops compositions based on HA and its derivatized forms are extensively characterized providing data on the tribological and mucoadhesive properties.
34680064	4	50	theme	tribological	494:505	arg1	properties					524:533	the tribological and mucoadhesive properties	490:533	the tribological and mucoadhesive properties	490:533	Therefore, eye drops compositions based on HA and its derivatized forms are extensively characterized providing data on the tribological and mucoadhesive properties.
34680064	3	51	theme	large	263:267	arg1	portion					269:275	a large portion	261:275	a large portion of the current literature on friction and lubrication	261:329	However, a large portion of the current literature on friction and lubrication addresses articular (joint) cartilage.
34680064	3	52	from	portion	269:275	arg1	lubrication					319:329	lubrication	319:329	lubrication	319:329	However, a large portion of the current literature on friction and lubrication addresses articular (joint) cartilage.
34680064	3	52	from	portion	269:275	arg1	friction					306:313	friction	306:313	friction	306:313	However, a large portion of the current literature on friction and lubrication addresses articular (joint) cartilage.
34680064	2	53	theme	High	182:185	arg1	HA					212:213	High and low molecular weight HA	182:213	High and low molecular weight HA	182:213	High and low molecular weight HA are currently used in ophthalmology.
34680064	6	54	theme	tribological	639:650	arg1	investigation					652:664	The tribological investigation	635:664	The tribological investigation	635:664	The tribological investigation reveals that amphiphilic HA-C12 decreases the friction coefficient.
34680064	11	55	theme	molecular	1135:1143	arg1	weight					1145:1150	Medium molecular weight	1128:1150	Medium molecular weight HA	1128:1153	Medium molecular weight HA shows significantly lower friction coefficient than high molecular weight HA.
34680064	11	56	theme	weight	1145:1150	arg1	HA					1152:1153	Medium molecular weight HA	1128:1153	Medium molecular weight HA	1128:1153	Medium molecular weight HA shows significantly lower friction coefficient than high molecular weight HA.
34680064	0	57	dep	Lubrication	17:27	arg1	the					13:15	the	13:15	the	13:15	Insight into the Lubrication and Adhesion Properties of Hyaluronan for Ocular Drug Delivery.
34680064	8	58	theme	eye	913:915	arg1	surface					898:904	the surface	894:904	the surface of the eye	894:915	Thus, it is predicted a prolonged residence time on the surface of the eye.
34680064	10	59	theme	trehalose	1082:1090	arg1	presence					1070:1077	The presence	1066:1077	The presence of trehalose	1066:1090	The presence of trehalose increases the friction coefficient.
34680064	9	60	theme	cells	1004:1008	arg1	protection					962:971	the protection	958:971	the protection of human keratinocytes (HaCaT) cells	958:1008	The incorporation of trehalose enhances the protection of human keratinocytes (HaCaT) cells, as demonstrated in an in-vitro cell-desiccation model.
34680064	4	61	theme	drops	385:389	arg1	compositions					391:402	eye drops compositions	381:402	eye drops compositions based on HA and its derivatized forms	381:440	Therefore, eye drops compositions based on HA and its derivatized forms are extensively characterized providing data on the tribological and mucoadhesive properties.
34680064	12	62	theme	forms	1304:1308	arg1	features					1281:1288	features	1281:1288	features of diverse HA forms	1281:1308	This research represents a first, wide array of features of diverse HA forms for eye drops contributing to increase the knowledge of these preparations.
34680064	12	63	theme	first	1260:1264	arg1	array					1272:1276	a first, wide array	1258:1276	array	1272:1276	This research represents a first, wide array of features of diverse HA forms for eye drops contributing to increase the knowledge of these preparations.
34680064	4	64	theme	eye	381:383	arg1	compositions					391:402	eye drops compositions	381:402	eye drops compositions based on HA and its derivatized forms	381:440	Therefore, eye drops compositions based on HA and its derivatized forms are extensively characterized providing data on the tribological and mucoadhesive properties.
34680064	11	65	theme	Medium	1128:1133	arg1	weight					1145:1150	Medium molecular weight	1128:1150	Medium molecular weight HA	1128:1153	Medium molecular weight HA shows significantly lower friction coefficient than high molecular weight HA.
34680064	12	66	theme	wide	1267:1270	arg1	array					1272:1276	a first, wide array	1258:1276	array	1272:1276	This research represents a first, wide array of features of diverse HA forms for eye drops contributing to increase the knowledge of these preparations.
34680064	7	67	theme	same	741:744	arg1	time					746:749	the same time	737:749	the same time	737:749	At the same time, the combination of trehalose/HA or HAC12 enhances up to eighty-fold the mucoadhesiveness.
34680064	3	68	theme	current	284:290	arg1	literature					292:301	the current literature	280:301	the current literature on friction and lubrication	280:329	However, a large portion of the current literature on friction and lubrication addresses articular (joint) cartilage.
34680064	2	69	theme	weight	205:210	arg1	HA					212:213	High and low molecular weight HA	182:213	High and low molecular weight HA	182:213	High and low molecular weight HA are currently used in ophthalmology.
34680064	2	70	used	used	229:232	arg2	HA					212:213	High and low molecular weight HA	182:213	High and low molecular weight HA	182:213	High and low molecular weight HA are currently used in ophthalmology.
33667610	1	0	theme	13C	333:335	arg1	spectroscopy					341:352	13C NMR spectroscopy	333:352	13C NMR spectroscopy	333:352	The K92 capsular polysaccharide (CPS) from Acinetobacter baumannii B8300 was studied by sugar analysis, Smith degradation, and one- and two-dimensional 1H and 13C NMR spectroscopy.
33667610	6	1	theme	wzi	1347:1349	arg1	gene					1351:1354	a wzi gene	1345:1354	a wzi gene predicted to be involved in the attachment of CPS to the cell surface	1345:1424	This module also includes an unknown open reading frame (orfKL92), a gtr166 glycosyltransferase gene, and a wzi gene predicted to be involved in the attachment of CPS to the cell surface.
33667610	2	2	theme	pentasaccharide	394:408	arg1	unit					417:420	a branched pentasaccharide repeat unit	383:420	a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues	383:467	The elucidated CPS includes a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues; an atypical composition given that all A. baumannii CPS structures determined to date contain at least one amino sugar.
33667610	1	3	theme	NMR	337:339	arg1	spectroscopy					341:352	13C NMR spectroscopy	333:352	13C NMR spectroscopy	333:352	The K92 capsular polysaccharide (CPS) from Acinetobacter baumannii B8300 was studied by sugar analysis, Smith degradation, and one- and two-dimensional 1H and 13C NMR spectroscopy.
33667610	4	4	theme	gene	930:933	arg1	cluster					935:941	the KL92 capsule biosynthesis gene cluster	900:941	the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence	900:970	However, the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence includes a gene for a novel Itr type, ItrA4, which is predicted to begin synthesis of the K92 CPS by transferring D-Galp 1-phosphate to the UndP lipid carrier.
33667610	7	5	theme	orfKL92-gtr166-itrA4-wziKL92	1461:1488	arg1	origins					1450:1456	the origins	1446:1456	the origins of orfKL92-gtr166-itrA4-wziKL92	1446:1488	Investigation into the origins of orfKL92-gtr166-itrA4-wziKL92 revealed it might have originated from Acinetobacter junii.
33667610	1	6	theme	sugar	262:266	arg1	analysis					268:275	sugar analysis	262:275	sugar analysis	262:275	The K92 capsular polysaccharide (CPS) from Acinetobacter baumannii B8300 was studied by sugar analysis, Smith degradation, and one- and two-dimensional 1H and 13C NMR spectroscopy.
33667610	0	7	theme	amino	92:96	arg1	polysaccharide					125:138	an amino sugar-lacking K92 capsular polysaccharide	89:138	an amino sugar-lacking K92 capsular polysaccharide	89:138	A novel ItrA4 d-galactosyl 1-phosphate transferase is predicted to initiate synthesis of an amino sugar-lacking K92 capsular polysaccharide of Acinetobacter baumannii B8300.
33667610	3	8	theme	transfer	695:702	arg1	1-phosphate					704:714	transfer 1-phosphate	695:714	transfer 1-phosphate of either a 2-acetamido-2-deoxy-d-hexose, a 2-acetamido-2,6-dideoxy-d-hexose or a 2-acetamido-4-acylamino-2,4,6-trideoxy-d-hexose to an undecaprenyl phosphate (UndP) carrier	695:888	Accordingly, biosynthesis of A. baumannii CPS types are initiated by initiating transferases (Itrs) that transfer 1-phosphate of either a 2-acetamido-2-deoxy-d-hexose, a 2-acetamido-2,6-dideoxy-d-hexose or a 2-acetamido-4-acylamino-2,4,6-trideoxy-d-hexose to an undecaprenyl phosphate (UndP) carrier.
33667610	6	9	theme	open	1276:1279	arg1	orfKL92					1296:1302	orfKL92	1296:1302	orfKL92	1296:1302	This module also includes an unknown open reading frame (orfKL92), a gtr166 glycosyltransferase gene, and a wzi gene predicted to be involved in the attachment of CPS to the cell surface.
33667610	6	9	theme	open	1276:1279	arg1	frame					1289:1293	an unknown open reading frame	1265:1293	an unknown open reading frame (orfKL92)	1265:1303	This module also includes an unknown open reading frame (orfKL92), a gtr166 glycosyltransferase gene, and a wzi gene predicted to be involved in the attachment of CPS to the cell surface.
33667610	4	10	theme	D-Galp	1086:1091	arg1	1-phosphate					1093:1103	D-Galp 1-phosphate	1086:1103	D-Galp 1-phosphate to the UndP lipid carrier	1086:1129	However, the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence includes a gene for a novel Itr type, ItrA4, which is predicted to begin synthesis of the K92 CPS by transferring D-Galp 1-phosphate to the UndP lipid carrier.
33667610	4	11	theme	novel	994:998	arg1	type					1004:1007	a novel Itr type	992:1007	a novel Itr type	992:1007	However, the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence includes a gene for a novel Itr type, ItrA4, which is predicted to begin synthesis of the K92 CPS by transferring D-Galp 1-phosphate to the UndP lipid carrier.
33667610	4	11	theme	novel	994:998	arg1	ItrA4					1010:1014	ItrA4	1010:1014	ItrA4	1010:1014	However, the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence includes a gene for a novel Itr type, ItrA4, which is predicted to begin synthesis of the K92 CPS by transferring D-Galp 1-phosphate to the UndP lipid carrier.
33667610	3	12	theme	2-acetamido-2-deoxy-d-hexose	728:755	arg1	1-phosphate					704:714	transfer 1-phosphate	695:714	transfer 1-phosphate of either a 2-acetamido-2-deoxy-d-hexose, a 2-acetamido-2,6-dideoxy-d-hexose or a 2-acetamido-4-acylamino-2,4,6-trideoxy-d-hexose to an undecaprenyl phosphate (UndP) carrier	695:888	Accordingly, biosynthesis of A. baumannii CPS types are initiated by initiating transferases (Itrs) that transfer 1-phosphate of either a 2-acetamido-2-deoxy-d-hexose, a 2-acetamido-2,6-dideoxy-d-hexose or a 2-acetamido-4-acylamino-2,4,6-trideoxy-d-hexose to an undecaprenyl phosphate (UndP) carrier.
33667610	6	13	theme	unknown	1268:1274	arg1	orfKL92					1296:1302	orfKL92	1296:1302	orfKL92	1296:1302	This module also includes an unknown open reading frame (orfKL92), a gtr166 glycosyltransferase gene, and a wzi gene predicted to be involved in the attachment of CPS to the cell surface.
33667610	6	13	theme	unknown	1268:1274	arg1	frame					1289:1293	an unknown open reading frame	1265:1293	an unknown open reading frame (orfKL92)	1265:1303	This module also includes an unknown open reading frame (orfKL92), a gtr166 glycosyltransferase gene, and a wzi gene predicted to be involved in the attachment of CPS to the cell surface.
33667610	5	14	located	found	1151:1155	arg1	module					1162:1167	a module	1160:1167	a module transcribed in the opposite direction to the majority of the K locus	1160:1236	The itrA4 gene was found in a module transcribed in the opposite direction to the majority of the K locus.
33667610	5	14	located	found	1151:1155	arg2	gene					1142:1145	The itrA4 gene	1132:1145	The itrA4 gene	1132:1145	The itrA4 gene was found in a module transcribed in the opposite direction to the majority of the K locus.
33667610	0	15	theme	K92	112:114	arg1	polysaccharide					125:138	an amino sugar-lacking K92 capsular polysaccharide	89:138	an amino sugar-lacking K92 capsular polysaccharide	89:138	A novel ItrA4 d-galactosyl 1-phosphate transferase is predicted to initiate synthesis of an amino sugar-lacking K92 capsular polysaccharide of Acinetobacter baumannii B8300.
33667610	4	16	theme	KL92	904:907	arg1	cluster					935:941	the KL92 capsule biosynthesis gene cluster	900:941	the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence	900:970	However, the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence includes a gene for a novel Itr type, ItrA4, which is predicted to begin synthesis of the K92 CPS by transferring D-Galp 1-phosphate to the UndP lipid carrier.
33667610	1	17	theme	K92	178:180	arg1	polysaccharide					191:204	The K92 capsular polysaccharide	174:204	The K92 capsular polysaccharide (CPS) from Acinetobacter baumannii B8300	174:245	The K92 capsular polysaccharide (CPS) from Acinetobacter baumannii B8300 was studied by sugar analysis, Smith degradation, and one- and two-dimensional 1H and 13C NMR spectroscopy.
33667610	1	17	theme	K92	178:180	arg1	CPS					207:209	CPS	207:209	CPS	207:209	The K92 capsular polysaccharide (CPS) from Acinetobacter baumannii B8300 was studied by sugar analysis, Smith degradation, and one- and two-dimensional 1H and 13C NMR spectroscopy.
33667610	0	18	theme	sugar-lacking	98:110	arg1	polysaccharide					125:138	an amino sugar-lacking K92 capsular polysaccharide	89:138	an amino sugar-lacking K92 capsular polysaccharide	89:138	A novel ItrA4 d-galactosyl 1-phosphate transferase is predicted to initiate synthesis of an amino sugar-lacking K92 capsular polysaccharide of Acinetobacter baumannii B8300.
33667610	2	19	contain	contain	556:562	arg2	sugar					583:587	at least one amino sugar	564:587	at least one amino sugar	564:587	The elucidated CPS includes a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues; an atypical composition given that all A. baumannii CPS structures determined to date contain at least one amino sugar.
33667610	2	19	contain	contain	556:562	arg1	structures					526:535	all A. baumannii CPS structures	505:535	all A. baumannii CPS structures determined to date	505:554	The elucidated CPS includes a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues; an atypical composition given that all A. baumannii CPS structures determined to date contain at least one amino sugar.
33667610	1	20	theme	capsular	182:189	arg1	polysaccharide					191:204	The K92 capsular polysaccharide	174:204	The K92 capsular polysaccharide (CPS) from Acinetobacter baumannii B8300	174:245	The K92 capsular polysaccharide (CPS) from Acinetobacter baumannii B8300 was studied by sugar analysis, Smith degradation, and one- and two-dimensional 1H and 13C NMR spectroscopy.
33667610	1	20	theme	capsular	182:189	arg1	CPS					207:209	CPS	207:209	CPS	207:209	The K92 capsular polysaccharide (CPS) from Acinetobacter baumannii B8300 was studied by sugar analysis, Smith degradation, and one- and two-dimensional 1H and 13C NMR spectroscopy.
33667610	0	21	theme	novel	2:6	arg1	transferase					39:49	A novel ItrA4 d-galactosyl 1-phosphate transferase	0:49	A novel ItrA4 d-galactosyl 1-phosphate transferase	0:49	A novel ItrA4 d-galactosyl 1-phosphate transferase is predicted to initiate synthesis of an amino sugar-lacking K92 capsular polysaccharide of Acinetobacter baumannii B8300.
33667610	5	22	theme	itrA4	1136:1140	arg1	gene					1142:1145	The itrA4 gene	1132:1145	The itrA4 gene	1132:1145	The itrA4 gene was found in a module transcribed in the opposite direction to the majority of the K locus.
33667610	4	23	theme	biosynthesis	917:928	arg1	cluster					935:941	the KL92 capsule biosynthesis gene cluster	900:941	the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence	900:970	However, the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence includes a gene for a novel Itr type, ItrA4, which is predicted to begin synthesis of the K92 CPS by transferring D-Galp 1-phosphate to the UndP lipid carrier.
33667610	4	24	theme	Itr	1000:1002	arg1	type					1004:1007	a novel Itr type	992:1007	a novel Itr type	992:1007	However, the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence includes a gene for a novel Itr type, ItrA4, which is predicted to begin synthesis of the K92 CPS by transferring D-Galp 1-phosphate to the UndP lipid carrier.
33667610	4	24	theme	Itr	1000:1002	arg1	ItrA4					1010:1014	ItrA4	1010:1014	ItrA4	1010:1014	However, the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence includes a gene for a novel Itr type, ItrA4, which is predicted to begin synthesis of the K92 CPS by transferring D-Galp 1-phosphate to the UndP lipid carrier.
33667610	2	25	theme	amino	577:581	arg1	sugar					583:587	at least one amino sugar	564:587	at least one amino sugar	564:587	The elucidated CPS includes a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues; an atypical composition given that all A. baumannii CPS structures determined to date contain at least one amino sugar.
33667610	4	26	from	cluster	935:941	arg1	sequence					963:970	the B8300 genome sequence	946:970	the B8300 genome sequence	946:970	However, the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence includes a gene for a novel Itr type, ItrA4, which is predicted to begin synthesis of the K92 CPS by transferring D-Galp 1-phosphate to the UndP lipid carrier.
33667610	1	27	theme	Smith	278:282	arg1	degradation					284:294	Smith degradation	278:294	Smith degradation	278:294	The K92 capsular polysaccharide (CPS) from Acinetobacter baumannii B8300 was studied by sugar analysis, Smith degradation, and one- and two-dimensional 1H and 13C NMR spectroscopy.
33667610	4	28	theme	capsule	909:915	arg1	cluster					935:941	the KL92 capsule biosynthesis gene cluster	900:941	the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence	900:970	However, the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence includes a gene for a novel Itr type, ItrA4, which is predicted to begin synthesis of the K92 CPS by transferring D-Galp 1-phosphate to the UndP lipid carrier.
33667610	6	29	theme	glycosyltransferase	1315:1333	arg1	gene					1335:1338	a gtr166 glycosyltransferase gene	1306:1338	a gtr166 glycosyltransferase gene	1306:1338	This module also includes an unknown open reading frame (orfKL92), a gtr166 glycosyltransferase gene, and a wzi gene predicted to be involved in the attachment of CPS to the cell surface.
33667610	6	30	theme	reading	1281:1287	arg1	orfKL92					1296:1302	orfKL92	1296:1302	orfKL92	1296:1302	This module also includes an unknown open reading frame (orfKL92), a gtr166 glycosyltransferase gene, and a wzi gene predicted to be involved in the attachment of CPS to the cell surface.
33667610	6	30	theme	reading	1281:1287	arg1	frame					1289:1293	an unknown open reading frame	1265:1293	an unknown open reading frame (orfKL92)	1265:1303	This module also includes an unknown open reading frame (orfKL92), a gtr166 glycosyltransferase gene, and a wzi gene predicted to be involved in the attachment of CPS to the cell surface.
33667610	0	31	theme	d-galactosyl	14:25	arg1	transferase					39:49	A novel ItrA4 d-galactosyl 1-phosphate transferase	0:49	A novel ItrA4 d-galactosyl 1-phosphate transferase	0:49	A novel ItrA4 d-galactosyl 1-phosphate transferase is predicted to initiate synthesis of an amino sugar-lacking K92 capsular polysaccharide of Acinetobacter baumannii B8300.
33667610	5	32	theme	opposite	1188:1195	arg1	direction					1197:1205	the opposite direction	1184:1205	the opposite direction to the majority of the K locus	1184:1236	The itrA4 gene was found in a module transcribed in the opposite direction to the majority of the K locus.
33667610	0	33	theme	polysaccharide	125:138	arg1	synthesis					76:84	synthesis	76:84	synthesis of an amino sugar-lacking K92 capsular polysaccharide of Acinetobacter baumannii B8300	76:171	A novel ItrA4 d-galactosyl 1-phosphate transferase is predicted to initiate synthesis of an amino sugar-lacking K92 capsular polysaccharide of Acinetobacter baumannii B8300.
33667610	0	34	theme	ItrA4	8:12	arg1	transferase					39:49	A novel ItrA4 d-galactosyl 1-phosphate transferase	0:49	A novel ItrA4 d-galactosyl 1-phosphate transferase	0:49	A novel ItrA4 d-galactosyl 1-phosphate transferase is predicted to initiate synthesis of an amino sugar-lacking K92 capsular polysaccharide of Acinetobacter baumannii B8300.
33667610	6	35	theme	CPS	1402:1404	arg1	attachment					1388:1397	the attachment	1384:1397	the attachment of CPS to the cell surface	1384:1424	This module also includes an unknown open reading frame (orfKL92), a gtr166 glycosyltransferase gene, and a wzi gene predicted to be involved in the attachment of CPS to the cell surface.
33667610	0	36	theme	capsular	116:123	arg1	polysaccharide					125:138	an amino sugar-lacking K92 capsular polysaccharide	89:138	an amino sugar-lacking K92 capsular polysaccharide	89:138	A novel ItrA4 d-galactosyl 1-phosphate transferase is predicted to initiate synthesis of an amino sugar-lacking K92 capsular polysaccharide of Acinetobacter baumannii B8300.
33667610	2	37	theme	atypical	473:480	arg1	composition					482:492	an atypical composition	470:492	an atypical composition given that all A. baumannii CPS structures determined to date contain at least one amino sugar	470:587	The elucidated CPS includes a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues; an atypical composition given that all A. baumannii CPS structures determined to date contain at least one amino sugar.
33667610	0	38	theme	Acinetobacter	143:155	arg1	baumannii					157:165	Acinetobacter baumannii B8300	143:171	Acinetobacter baumannii B8300	143:171	A novel ItrA4 d-galactosyl 1-phosphate transferase is predicted to initiate synthesis of an amino sugar-lacking K92 capsular polysaccharide of Acinetobacter baumannii B8300.
33667610	7	39	theme	Acinetobacter	1529:1541	arg1	junii					1543:1547	Acinetobacter junii	1529:1547	Acinetobacter junii	1529:1547	Investigation into the origins of orfKL92-gtr166-itrA4-wziKL92 revealed it might have originated from Acinetobacter junii.
33667610	4	40	theme	UndP	1112:1115	arg1	carrier					1123:1129	the UndP lipid carrier	1108:1129	the UndP lipid carrier	1108:1129	However, the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence includes a gene for a novel Itr type, ItrA4, which is predicted to begin synthesis of the K92 CPS by transferring D-Galp 1-phosphate to the UndP lipid carrier.
33667610	0	41	theme	1-phosphate	27:37	arg1	transferase					39:49	A novel ItrA4 d-galactosyl 1-phosphate transferase	0:49	A novel ItrA4 d-galactosyl 1-phosphate transferase	0:49	A novel ItrA4 d-galactosyl 1-phosphate transferase is predicted to initiate synthesis of an amino sugar-lacking K92 capsular polysaccharide of Acinetobacter baumannii B8300.
33667610	1	42	theme	one-	301:304	arg1	1H					326:327	one- and two-dimensional 1H	301:327	one- and two-dimensional 1H	301:327	The K92 capsular polysaccharide (CPS) from Acinetobacter baumannii B8300 was studied by sugar analysis, Smith degradation, and one- and two-dimensional 1H and 13C NMR spectroscopy.
33667610	3	43	theme	types	636:640	arg1	biosynthesis					603:614	biosynthesis	603:614	biosynthesis of A. baumannii CPS types	603:640	Accordingly, biosynthesis of A. baumannii CPS types are initiated by initiating transferases (Itrs) that transfer 1-phosphate of either a 2-acetamido-2-deoxy-d-hexose, a 2-acetamido-2,6-dideoxy-d-hexose or a 2-acetamido-4-acylamino-2,4,6-trideoxy-d-hexose to an undecaprenyl phosphate (UndP) carrier.
33667610	3	44	theme	2-acetamido-4-acylamino-2,4,6-trideoxy-d-hexose	798:844	arg1	1-phosphate					704:714	transfer 1-phosphate	695:714	transfer 1-phosphate of either a 2-acetamido-2-deoxy-d-hexose, a 2-acetamido-2,6-dideoxy-d-hexose or a 2-acetamido-4-acylamino-2,4,6-trideoxy-d-hexose to an undecaprenyl phosphate (UndP) carrier	695:888	Accordingly, biosynthesis of A. baumannii CPS types are initiated by initiating transferases (Itrs) that transfer 1-phosphate of either a 2-acetamido-2-deoxy-d-hexose, a 2-acetamido-2,6-dideoxy-d-hexose or a 2-acetamido-4-acylamino-2,4,6-trideoxy-d-hexose to an undecaprenyl phosphate (UndP) carrier.
33667610	4	45	theme	CPS	1066:1068	arg1	synthesis					1045:1053	synthesis	1045:1053	synthesis of the K92 CPS	1045:1068	However, the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence includes a gene for a novel Itr type, ItrA4, which is predicted to begin synthesis of the K92 CPS by transferring D-Galp 1-phosphate to the UndP lipid carrier.
33667610	1	46	theme	Acinetobacter	217:229	arg1	baumannii					231:239	Acinetobacter baumannii B8300	217:245	Acinetobacter baumannii B8300	217:245	The K92 capsular polysaccharide (CPS) from Acinetobacter baumannii B8300 was studied by sugar analysis, Smith degradation, and one- and two-dimensional 1H and 13C NMR spectroscopy.
33667610	3	47	theme	undecaprenyl	852:863	arg1	UndP					876:879	UndP	876:879	UndP	876:879	Accordingly, biosynthesis of A. baumannii CPS types are initiated by initiating transferases (Itrs) that transfer 1-phosphate of either a 2-acetamido-2-deoxy-d-hexose, a 2-acetamido-2,6-dideoxy-d-hexose or a 2-acetamido-4-acylamino-2,4,6-trideoxy-d-hexose to an undecaprenyl phosphate (UndP) carrier.
33667610	3	47	theme	undecaprenyl	852:863	arg1	phosphate					865:873	undecaprenyl phosphate	852:873	an undecaprenyl phosphate (UndP) carrier	849:888	Accordingly, biosynthesis of A. baumannii CPS types are initiated by initiating transferases (Itrs) that transfer 1-phosphate of either a 2-acetamido-2-deoxy-d-hexose, a 2-acetamido-2,6-dideoxy-d-hexose or a 2-acetamido-4-acylamino-2,4,6-trideoxy-d-hexose to an undecaprenyl phosphate (UndP) carrier.
33667610	4	48	theme	genome	956:961	arg1	sequence					963:970	the B8300 genome sequence	946:970	the B8300 genome sequence	946:970	However, the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence includes a gene for a novel Itr type, ItrA4, which is predicted to begin synthesis of the K92 CPS by transferring D-Galp 1-phosphate to the UndP lipid carrier.
33667610	6	49	theme	gtr166	1308:1313	arg1	gene					1335:1338	a gtr166 glycosyltransferase gene	1306:1338	a gtr166 glycosyltransferase gene	1306:1338	This module also includes an unknown open reading frame (orfKL92), a gtr166 glycosyltransferase gene, and a wzi gene predicted to be involved in the attachment of CPS to the cell surface.
33667610	6	50	attach	attachment	1388:1397	arg2	CPS					1402:1404	CPS	1402:1404	CPS	1402:1404	This module also includes an unknown open reading frame (orfKL92), a gtr166 glycosyltransferase gene, and a wzi gene predicted to be involved in the attachment of CPS to the cell surface.
33667610	6	50	attach	attachment	1388:1397	arg1	surface					1418:1424	the cell surface	1409:1424	the cell surface	1409:1424	This module also includes an unknown open reading frame (orfKL92), a gtr166 glycosyltransferase gene, and a wzi gene predicted to be involved in the attachment of CPS to the cell surface.
33667610	1	51	theme	two-dimensional	310:324	arg1	1H					326:327	one- and two-dimensional 1H	301:327	one- and two-dimensional 1H	301:327	The K92 capsular polysaccharide (CPS) from Acinetobacter baumannii B8300 was studied by sugar analysis, Smith degradation, and one- and two-dimensional 1H and 13C NMR spectroscopy.
33667610	0	52	theme	baumannii	157:165	arg1	synthesis					76:84	synthesis	76:84	synthesis of an amino sugar-lacking K92 capsular polysaccharide of Acinetobacter baumannii B8300	76:171	A novel ItrA4 d-galactosyl 1-phosphate transferase is predicted to initiate synthesis of an amino sugar-lacking K92 capsular polysaccharide of Acinetobacter baumannii B8300.
33667610	6	53	theme	cell	1413:1416	arg1	surface					1418:1424	the cell surface	1409:1424	the cell surface	1409:1424	This module also includes an unknown open reading frame (orfKL92), a gtr166 glycosyltransferase gene, and a wzi gene predicted to be involved in the attachment of CPS to the cell surface.
33667610	1	54	from	baumannii	231:239	arg1	polysaccharide					191:204	The K92 capsular polysaccharide	174:204	The K92 capsular polysaccharide (CPS) from Acinetobacter baumannii B8300	174:245	The K92 capsular polysaccharide (CPS) from Acinetobacter baumannii B8300 was studied by sugar analysis, Smith degradation, and one- and two-dimensional 1H and 13C NMR spectroscopy.
33667610	1	54	from	baumannii	231:239	arg1	CPS					207:209	CPS	207:209	CPS	207:209	The K92 capsular polysaccharide (CPS) from Acinetobacter baumannii B8300 was studied by sugar analysis, Smith degradation, and one- and two-dimensional 1H and 13C NMR spectroscopy.
33667610	4	55	theme	K92	1062:1064	arg1	CPS					1066:1068	the K92 CPS	1058:1068	the K92 CPS	1058:1068	However, the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence includes a gene for a novel Itr type, ItrA4, which is predicted to begin synthesis of the K92 CPS by transferring D-Galp 1-phosphate to the UndP lipid carrier.
33667610	3	56	theme	CPS	632:634	arg1	types					636:640	A. baumannii CPS types	619:640	A. baumannii CPS types	619:640	Accordingly, biosynthesis of A. baumannii CPS types are initiated by initiating transferases (Itrs) that transfer 1-phosphate of either a 2-acetamido-2-deoxy-d-hexose, a 2-acetamido-2,6-dideoxy-d-hexose or a 2-acetamido-4-acylamino-2,4,6-trideoxy-d-hexose to an undecaprenyl phosphate (UndP) carrier.
33667610	2	57	theme	A. baumannii	509:520	arg1	structures					526:535	all A. baumannii CPS structures	505:535	all A. baumannii CPS structures determined to date	505:554	The elucidated CPS includes a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues; an atypical composition given that all A. baumannii CPS structures determined to date contain at least one amino sugar.
33667610	5	58	theme	K	1230:1230	arg1	locus					1232:1236	the K locus	1226:1236	the K locus	1226:1236	The itrA4 gene was found in a module transcribed in the opposite direction to the majority of the K locus.
33667610	2	59	contain	containing	422:431	arg1	unit					417:420	a branched pentasaccharide repeat unit	383:420	a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues	383:467	The elucidated CPS includes a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues; an atypical composition given that all A. baumannii CPS structures determined to date contain at least one amino sugar.
33667610	2	59	contain	containing	422:431	arg2	residues					460:467	four l-Rhap residues	448:467	four l-Rhap residues	448:467	The elucidated CPS includes a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues; an atypical composition given that all A. baumannii CPS structures determined to date contain at least one amino sugar.
33667610	2	59	contain	containing	422:431	arg2	d-Galp					437:442	one d-Galp	433:442	one d-Galp	433:442	The elucidated CPS includes a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues; an atypical composition given that all A. baumannii CPS structures determined to date contain at least one amino sugar.
33667610	2	60	dep	includes	374:381	arg1	composition					482:492	an atypical composition	470:492	an atypical composition given that all A. baumannii CPS structures determined to date contain at least one amino sugar	470:587	The elucidated CPS includes a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues; an atypical composition given that all A. baumannii CPS structures determined to date contain at least one amino sugar.
33667610	2	61	theme	elucidated	359:368	arg1	CPS					370:372	The elucidated CPS	355:372	The elucidated CPS	355:372	The elucidated CPS includes a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues; an atypical composition given that all A. baumannii CPS structures determined to date contain at least one amino sugar.
33667610	4	62	theme	lipid	1117:1121	arg1	carrier					1123:1129	the UndP lipid carrier	1108:1129	the UndP lipid carrier	1108:1129	However, the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence includes a gene for a novel Itr type, ItrA4, which is predicted to begin synthesis of the K92 CPS by transferring D-Galp 1-phosphate to the UndP lipid carrier.
33667610	5	63	theme	locus	1232:1236	arg1	majority					1214:1221	the majority	1210:1221	the majority of the K locus	1210:1236	The itrA4 gene was found in a module transcribed in the opposite direction to the majority of the K locus.
33667610	2	64	theme	CPS	522:524	arg1	structures					526:535	all A. baumannii CPS structures	505:535	all A. baumannii CPS structures determined to date	505:554	The elucidated CPS includes a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues; an atypical composition given that all A. baumannii CPS structures determined to date contain at least one amino sugar.
33667610	2	65	theme	repeat	410:415	arg1	unit					417:420	a branched pentasaccharide repeat unit	383:420	a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues	383:467	The elucidated CPS includes a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues; an atypical composition given that all A. baumannii CPS structures determined to date contain at least one amino sugar.
33667610	4	66	theme	B8300	950:954	arg1	sequence					963:970	the B8300 genome sequence	946:970	the B8300 genome sequence	946:970	However, the KL92 capsule biosynthesis gene cluster in the B8300 genome sequence includes a gene for a novel Itr type, ItrA4, which is predicted to begin synthesis of the K92 CPS by transferring D-Galp 1-phosphate to the UndP lipid carrier.
33667610	2	67	theme	branched	385:392	arg1	unit					417:420	a branched pentasaccharide repeat unit	383:420	a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues	383:467	The elucidated CPS includes a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues; an atypical composition given that all A. baumannii CPS structures determined to date contain at least one amino sugar.
33667610	3	68	theme	phosphate	865:873	arg1	carrier					882:888	an undecaprenyl phosphate (UndP) carrier	849:888	an undecaprenyl phosphate (UndP) carrier	849:888	Accordingly, biosynthesis of A. baumannii CPS types are initiated by initiating transferases (Itrs) that transfer 1-phosphate of either a 2-acetamido-2-deoxy-d-hexose, a 2-acetamido-2,6-dideoxy-d-hexose or a 2-acetamido-4-acylamino-2,4,6-trideoxy-d-hexose to an undecaprenyl phosphate (UndP) carrier.
33667610	3	69	theme	A. baumannii	619:630	arg1	types					636:640	A. baumannii CPS types	619:640	A. baumannii CPS types	619:640	Accordingly, biosynthesis of A. baumannii CPS types are initiated by initiating transferases (Itrs) that transfer 1-phosphate of either a 2-acetamido-2-deoxy-d-hexose, a 2-acetamido-2,6-dideoxy-d-hexose or a 2-acetamido-4-acylamino-2,4,6-trideoxy-d-hexose to an undecaprenyl phosphate (UndP) carrier.
33667610	2	70	theme	l-Rhap	453:458	arg1	residues					460:467	four l-Rhap residues	448:467	four l-Rhap residues	448:467	The elucidated CPS includes a branched pentasaccharide repeat unit containing one d-Galp and four l-Rhap residues; an atypical composition given that all A. baumannii CPS structures determined to date contain at least one amino sugar.
33600188	8	0	theme	future	1588:1593	arg1	study					1595:1599	any future study	1584:1599	any future study of the Gram-negative bacteria	1584:1629	These findings present a possible explanation for Vibrio cholerae's immune system evasion properties and establish the differences between the lipid A types, which should be of use for any future study of the Gram-negative bacteria.
33600188	3	1	theme	cholera	644:650	arg1	pandemic					652:659	the current cholera pandemic	632:659	the current cholera pandemic	632:659	Particularly, in Vibrio cholerae, a Gram-negative bacterium responsible for cholera, a severe diarrheal disease, modifications of lipid A structures grant antibiotic resistance and are a primary factor that led to the current cholera pandemic.
33600188	3	2	theme	responsible	478:488	arg1	cholerae					442:449	Vibrio cholerae	435:449	Vibrio cholerae	435:449	Particularly, in Vibrio cholerae, a Gram-negative bacterium responsible for cholera, a severe diarrheal disease, modifications of lipid A structures grant antibiotic resistance and are a primary factor that led to the current cholera pandemic.
33600188	3	2	theme	responsible	478:488	arg1	bacterium					468:476	a Gram-negative bacterium	452:476	a Gram-negative bacterium responsible for cholera, a severe diarrheal disease	452:528	Particularly, in Vibrio cholerae, a Gram-negative bacterium responsible for cholera, a severe diarrheal disease, modifications of lipid A structures grant antibiotic resistance and are a primary factor that led to the current cholera pandemic.
33600188	6	3	from	differences	1201:1211	arg1	composition					1216:1226	composition	1216:1226	composition	1216:1226	All-atom molecular dynamics simulations reveal that membrane properties such as hydrophobic thickness, acyl chain order parameter, and area per lipid are largely impacted by lipid A modifications due to differences in composition and acyl chain distortions.
33600188	6	3	from	differences	1201:1211	arg1	distortions					1243:1253	acyl chain distortions	1232:1253	acyl chain distortions	1232:1253	All-atom molecular dynamics simulations reveal that membrane properties such as hydrophobic thickness, acyl chain order parameter, and area per lipid are largely impacted by lipid A modifications due to differences in composition and acyl chain distortions.
33600188	6	4	theme	acyl	1232:1235	arg1	distortions					1243:1253	acyl chain distortions	1232:1253	acyl chain distortions	1232:1253	All-atom molecular dynamics simulations reveal that membrane properties such as hydrophobic thickness, acyl chain order parameter, and area per lipid are largely impacted by lipid A modifications due to differences in composition and acyl chain distortions.
33600188	3	5	theme	diarrheal	512:520	arg1	disease					522:528	a severe diarrheal disease	503:528	a severe diarrheal disease	503:528	Particularly, in Vibrio cholerae, a Gram-negative bacterium responsible for cholera, a severe diarrheal disease, modifications of lipid A structures grant antibiotic resistance and are a primary factor that led to the current cholera pandemic.
33600188	3	5	theme	diarrheal	512:520	arg1	cholera					494:500	cholera	494:500	cholera	494:500	Particularly, in Vibrio cholerae, a Gram-negative bacterium responsible for cholera, a severe diarrheal disease, modifications of lipid A structures grant antibiotic resistance and are a primary factor that led to the current cholera pandemic.
33600188	1	6	theme	immune	261:266	arg1	recognition					275:285	immune system recognition	261:285	antibiotic permeability as well as immune system recognition	226:285	Lipopolysaccharides (LPS) present in the outer leaflet of Gram-negative bacterial outer membranes protect the bacteria from external threats and influence antibiotic permeability as well as immune system recognition.
33600188	1	7	theme	membranes	159:167	arg1	leaflet					118:124	the outer leaflet	108:124	the outer leaflet of Gram-negative bacterial outer membranes	108:167	Lipopolysaccharides (LPS) present in the outer leaflet of Gram-negative bacterial outer membranes protect the bacteria from external threats and influence antibiotic permeability as well as immune system recognition.
33600188	7	8	theme	antimicrobial	1368:1380	arg1	evasion					1390:1396	cationic antimicrobial peptide evasion	1359:1396	cationic antimicrobial peptide evasion	1359:1396	The modified lipid A is also less negatively charged, which possibly reveals a resistance mechanism to cationic antimicrobial peptide evasion.
33600188	2	9	theme	lipid	305:309	arg1	anchor					318:323	the anchor	314:323	the anchor of an LPS molecule to the outer membrane	314:364	The structure of lipid A, the anchor of an LPS molecule to the outer membrane, can make direct influences on membrane properties.
33600188	2	9	theme	lipid	305:309	arg1	A					311:311	lipid A	305:311	lipid A	305:311	The structure of lipid A, the anchor of an LPS molecule to the outer membrane, can make direct influences on membrane properties.
33600188	5	10	theme	lipid	838:842	arg1	structures					846:855	distinct lipid A structures	829:855	distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen	829:896	In this work, five symmetric bilayer systems comprised of distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen were modeled and simulated to explore influences of different lipid A types on membrane properties.
33600188	5	11	dep	Vibrio	860:865	arg1	LPS					876:878	LPS	876:878	Vibrio cholerae LPS with O1 O-antigen	860:896	In this work, five symmetric bilayer systems comprised of distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen were modeled and simulated to explore influences of different lipid A types on membrane properties.
33600188	5	11	dep	Vibrio	860:865	arg1	cholerae					867:874	Vibrio cholerae LPS with O1 O-antigen	860:896	Vibrio cholerae LPS with O1 O-antigen	860:896	In this work, five symmetric bilayer systems comprised of distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen were modeled and simulated to explore influences of different lipid A types on membrane properties.
33600188	5	12	theme	symmetric	790:798	arg1	systems					808:814	five symmetric bilayer systems	785:814	five symmetric bilayer systems comprised of distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen	785:896	In this work, five symmetric bilayer systems comprised of distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen were modeled and simulated to explore influences of different lipid A types on membrane properties.
33600188	7	13	theme	resistance	1335:1344	arg1	mechanism					1346:1354	a resistance mechanism	1333:1354	a resistance mechanism to cationic antimicrobial peptide evasion	1333:1396	The modified lipid A is also less negatively charged, which possibly reveals a resistance mechanism to cationic antimicrobial peptide evasion.
33600188	8	14	theme	possible	1424:1431	arg1	explanation					1433:1443	a possible explanation	1422:1443	a possible explanation for Vibrio cholerae's immune system evasion properties	1422:1498	These findings present a possible explanation for Vibrio cholerae's immune system evasion properties and establish the differences between the lipid A types, which should be of use for any future study of the Gram-negative bacteria.
33600188	3	15	theme	A	554:554	arg1	structures					556:565	lipid A structures	548:565	lipid A structures	548:565	Particularly, in Vibrio cholerae, a Gram-negative bacterium responsible for cholera, a severe diarrheal disease, modifications of lipid A structures grant antibiotic resistance and are a primary factor that led to the current cholera pandemic.
33600188	6	16	theme	lipid	1172:1176	arg1	modifications					1180:1192	lipid A modifications	1172:1192	lipid A modifications due to differences in composition and acyl chain distortions	1172:1253	All-atom molecular dynamics simulations reveal that membrane properties such as hydrophobic thickness, acyl chain order parameter, and area per lipid are largely impacted by lipid A modifications due to differences in composition and acyl chain distortions.
33600188	5	17	theme	O1	885:886	arg1	O-antigen					888:896	O1 O-antigen	885:896	O1 O-antigen	885:896	In this work, five symmetric bilayer systems comprised of distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen were modeled and simulated to explore influences of different lipid A types on membrane properties.
33600188	7	18	theme	lipid	1269:1273	arg1	charged					1301:1307	charged	1301:1307	charged	1301:1307	The modified lipid A is also less negatively charged, which possibly reveals a resistance mechanism to cationic antimicrobial peptide evasion.
33600188	7	18	theme	lipid	1269:1273	arg1	A					1275:1275	The modified lipid A	1256:1275	The modified lipid A	1256:1275	The modified lipid A is also less negatively charged, which possibly reveals a resistance mechanism to cationic antimicrobial peptide evasion.
33600188	0	19	theme	LPS	47:49	arg1	Properties					59:68	LPS Bilayer Properties	47:68	LPS Bilayer Properties	47:68	Influences of Vibrio cholerae Lipid A Types on LPS Bilayer Properties.
33600188	8	20	theme	immune	1467:1472	arg1	properties					1489:1498	Vibrio cholerae's immune system evasion properties	1449:1498	Vibrio cholerae's immune system evasion properties	1449:1498	These findings present a possible explanation for Vibrio cholerae's immune system evasion properties and establish the differences between the lipid A types, which should be of use for any future study of the Gram-negative bacteria.
33600188	1	21	theme	bacterial	143:151	arg1	membranes					159:167	Gram-negative bacterial outer membranes	129:167	Gram-negative bacterial outer membranes	129:167	Lipopolysaccharides (LPS) present in the outer leaflet of Gram-negative bacterial outer membranes protect the bacteria from external threats and influence antibiotic permeability as well as immune system recognition.
33600188	8	22	theme	evasion	1481:1487	arg1	properties					1489:1498	Vibrio cholerae's immune system evasion properties	1449:1498	Vibrio cholerae's immune system evasion properties	1449:1498	These findings present a possible explanation for Vibrio cholerae's immune system evasion properties and establish the differences between the lipid A types, which should be of use for any future study of the Gram-negative bacteria.
33600188	1	23	from	leaflet	118:124	arg1	present					97:103	present	97:103	present	97:103	Lipopolysaccharides (LPS) present in the outer leaflet of Gram-negative bacterial outer membranes protect the bacteria from external threats and influence antibiotic permeability as well as immune system recognition.
33600188	1	24	theme	outer	112:116	arg1	leaflet					118:124	the outer leaflet	108:124	the outer leaflet of Gram-negative bacterial outer membranes	108:167	Lipopolysaccharides (LPS) present in the outer leaflet of Gram-negative bacterial outer membranes protect the bacteria from external threats and influence antibiotic permeability as well as immune system recognition.
33600188	5	25	theme	Vibrio	860:865	arg1	structures					846:855	distinct lipid A structures	829:855	distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen	829:896	In this work, five symmetric bilayer systems comprised of distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen were modeled and simulated to explore influences of different lipid A types on membrane properties.
33600188	2	26	theme	outer	351:355	arg1	membrane					357:364	the outer membrane	347:364	the outer membrane	347:364	The structure of lipid A, the anchor of an LPS molecule to the outer membrane, can make direct influences on membrane properties.
33600188	1	27	theme	system	268:273	arg1	recognition					275:285	immune system recognition	261:285	antibiotic permeability as well as immune system recognition	226:285	Lipopolysaccharides (LPS) present in the outer leaflet of Gram-negative bacterial outer membranes protect the bacteria from external threats and influence antibiotic permeability as well as immune system recognition.
33600188	1	28	from	present	97:103	arg1	leaflet					118:124	the outer leaflet	108:124	the outer leaflet of Gram-negative bacterial outer membranes	108:167	Lipopolysaccharides (LPS) present in the outer leaflet of Gram-negative bacterial outer membranes protect the bacteria from external threats and influence antibiotic permeability as well as immune system recognition.
33600188	2	29	theme	membrane	397:404	arg1	properties					406:415	membrane properties	397:415	membrane properties	397:415	The structure of lipid A, the anchor of an LPS molecule to the outer membrane, can make direct influences on membrane properties.
33600188	0	30	theme	Vibrio	14:19	arg1	Types					38:42	Vibrio cholerae Lipid A Types	14:42	Vibrio cholerae Lipid A Types	14:42	Influences of Vibrio cholerae Lipid A Types on LPS Bilayer Properties.
33600188	8	31	dep	Vibrio	1449:1454	arg1	cholerae					1456:1463	Vibrio cholerae	1449:1463	Vibrio cholerae's immune system evasion properties	1449:1498	These findings present a possible explanation for Vibrio cholerae's immune system evasion properties and establish the differences between the lipid A types, which should be of use for any future study of the Gram-negative bacteria.
33600188	6	32	theme	molecular	1007:1015	arg1	dynamics					1017:1024	All-atom molecular dynamics	998:1024	All-atom molecular dynamics simulations	998:1036	All-atom molecular dynamics simulations reveal that membrane properties such as hydrophobic thickness, acyl chain order parameter, and area per lipid are largely impacted by lipid A modifications due to differences in composition and acyl chain distortions.
33600188	8	33	theme	lipid	1542:1546	arg1	A					1548:1548	lipid A	1542:1548	the lipid A types	1538:1554	These findings present a possible explanation for Vibrio cholerae's immune system evasion properties and establish the differences between the lipid A types, which should be of use for any future study of the Gram-negative bacteria.
33600188	3	34	from	grant	567:571	arg1	cholerae					442:449	Vibrio cholerae	435:449	Vibrio cholerae	435:449	Particularly, in Vibrio cholerae, a Gram-negative bacterium responsible for cholera, a severe diarrheal disease, modifications of lipid A structures grant antibiotic resistance and are a primary factor that led to the current cholera pandemic.
33600188	3	34	from	grant	567:571	arg1	bacterium					468:476	a Gram-negative bacterium	452:476	a Gram-negative bacterium responsible for cholera, a severe diarrheal disease	452:528	Particularly, in Vibrio cholerae, a Gram-negative bacterium responsible for cholera, a severe diarrheal disease, modifications of lipid A structures grant antibiotic resistance and are a primary factor that led to the current cholera pandemic.
33600188	0	35	theme	Lipid	30:34	arg1	Types					38:42	Vibrio cholerae Lipid A Types	14:42	Vibrio cholerae Lipid A Types	14:42	Influences of Vibrio cholerae Lipid A Types on LPS Bilayer Properties.
33600188	7	36	theme	modified	1260:1267	arg1	charged					1301:1307	charged	1301:1307	charged	1301:1307	The modified lipid A is also less negatively charged, which possibly reveals a resistance mechanism to cationic antimicrobial peptide evasion.
33600188	7	36	theme	modified	1260:1267	arg1	A					1275:1275	The modified lipid A	1256:1275	The modified lipid A	1256:1275	The modified lipid A is also less negatively charged, which possibly reveals a resistance mechanism to cationic antimicrobial peptide evasion.
33600188	3	37	theme	primary	605:611	arg1	factor					613:618	a primary factor	603:618	a primary factor that led to the current cholera pandemic	603:659	Particularly, in Vibrio cholerae, a Gram-negative bacterium responsible for cholera, a severe diarrheal disease, modifications of lipid A structures grant antibiotic resistance and are a primary factor that led to the current cholera pandemic.
33600188	0	38	theme	Types	38:42	arg1	Influences					0:9	Influences	0:9	Influences of Vibrio cholerae Lipid A Types on LPS Bilayer Properties.	0:69	Influences of Vibrio cholerae Lipid A Types on LPS Bilayer Properties.
33600188	6	39	theme	acyl	1101:1104	arg1	parameter					1118:1126	acyl chain order parameter	1101:1126	acyl chain order parameter	1101:1126	All-atom molecular dynamics simulations reveal that membrane properties such as hydrophobic thickness, acyl chain order parameter, and area per lipid are largely impacted by lipid A modifications due to differences in composition and acyl chain distortions.
33600188	3	40	theme	current	636:642	arg1	pandemic					652:659	the current cholera pandemic	632:659	the current cholera pandemic	632:659	Particularly, in Vibrio cholerae, a Gram-negative bacterium responsible for cholera, a severe diarrheal disease, modifications of lipid A structures grant antibiotic resistance and are a primary factor that led to the current cholera pandemic.
33600188	4	41	from	difference	675:684	arg1	properties					700:709	structural properties	689:709	structural properties incurred by such modifications	689:740	However, the difference in structural properties incurred by such modifications has not been fully explored.
33600188	5	42	theme	different	950:958	arg1	types					968:972	different lipid A types	950:972	different lipid A types	950:972	In this work, five symmetric bilayer systems comprised of distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen were modeled and simulated to explore influences of different lipid A types on membrane properties.
33600188	8	43	theme	bacteria	1622:1629	arg1	study					1595:1599	any future study	1584:1599	any future study of the Gram-negative bacteria	1584:1629	These findings present a possible explanation for Vibrio cholerae's immune system evasion properties and establish the differences between the lipid A types, which should be of use for any future study of the Gram-negative bacteria.
33600188	1	44	theme	antibiotic	226:235	arg1	permeability					237:248	antibiotic permeability	226:248	antibiotic permeability as well as immune system recognition	226:285	Lipopolysaccharides (LPS) present in the outer leaflet of Gram-negative bacterial outer membranes protect the bacteria from external threats and influence antibiotic permeability as well as immune system recognition.
33600188	5	45	theme	A	966:966	arg1	types					968:972	different lipid A types	950:972	different lipid A types	950:972	In this work, five symmetric bilayer systems comprised of distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen were modeled and simulated to explore influences of different lipid A types on membrane properties.
33600188	2	46	from	influences	383:392	arg1	properties					406:415	membrane properties	397:415	membrane properties	397:415	The structure of lipid A, the anchor of an LPS molecule to the outer membrane, can make direct influences on membrane properties.
33600188	1	47	attach	present	97:103	arg2	LPS					92:94	LPS	92:94	LPS	92:94	Lipopolysaccharides (LPS) present in the outer leaflet of Gram-negative bacterial outer membranes protect the bacteria from external threats and influence antibiotic permeability as well as immune system recognition.
33600188	1	47	attach	present	97:103	arg2	Lipopolysaccharides					71:89	Lipopolysaccharides	71:89	Lipopolysaccharides (LPS) present in the outer leaflet of Gram-negative bacterial outer membranes	71:167	Lipopolysaccharides (LPS) present in the outer leaflet of Gram-negative bacterial outer membranes protect the bacteria from external threats and influence antibiotic permeability as well as immune system recognition.
33600188	1	47	attach	present	97:103	arg1	leaflet					118:124	the outer leaflet	108:124	the outer leaflet of Gram-negative bacterial outer membranes	108:167	Lipopolysaccharides (LPS) present in the outer leaflet of Gram-negative bacterial outer membranes protect the bacteria from external threats and influence antibiotic permeability as well as immune system recognition.
33600188	2	48	theme	LPS	331:333	arg1	molecule					335:342	an LPS molecule	328:342	an LPS molecule	328:342	The structure of lipid A, the anchor of an LPS molecule to the outer membrane, can make direct influences on membrane properties.
33600188	5	49	from	influences	936:945	arg1	properties					986:995	membrane properties	977:995	membrane properties	977:995	In this work, five symmetric bilayer systems comprised of distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen were modeled and simulated to explore influences of different lipid A types on membrane properties.
33600188	6	50	theme	chain	1237:1241	arg1	distortions					1243:1253	acyl chain distortions	1232:1253	acyl chain distortions	1232:1253	All-atom molecular dynamics simulations reveal that membrane properties such as hydrophobic thickness, acyl chain order parameter, and area per lipid are largely impacted by lipid A modifications due to differences in composition and acyl chain distortions.
33600188	3	51	theme	severe	505:510	arg1	disease					522:528	a severe diarrheal disease	503:528	a severe diarrheal disease	503:528	Particularly, in Vibrio cholerae, a Gram-negative bacterium responsible for cholera, a severe diarrheal disease, modifications of lipid A structures grant antibiotic resistance and are a primary factor that led to the current cholera pandemic.
33600188	3	51	theme	severe	505:510	arg1	cholera					494:500	cholera	494:500	cholera	494:500	Particularly, in Vibrio cholerae, a Gram-negative bacterium responsible for cholera, a severe diarrheal disease, modifications of lipid A structures grant antibiotic resistance and are a primary factor that led to the current cholera pandemic.
33600188	3	52	from	factor	613:618	arg1	cholerae					442:449	Vibrio cholerae	435:449	Vibrio cholerae	435:449	Particularly, in Vibrio cholerae, a Gram-negative bacterium responsible for cholera, a severe diarrheal disease, modifications of lipid A structures grant antibiotic resistance and are a primary factor that led to the current cholera pandemic.
33600188	3	52	from	factor	613:618	arg1	bacterium					468:476	a Gram-negative bacterium	452:476	a Gram-negative bacterium responsible for cholera, a severe diarrheal disease	452:528	Particularly, in Vibrio cholerae, a Gram-negative bacterium responsible for cholera, a severe diarrheal disease, modifications of lipid A structures grant antibiotic resistance and are a primary factor that led to the current cholera pandemic.
33600188	1	53	theme	outer	153:157	arg1	membranes					159:167	Gram-negative bacterial outer membranes	129:167	Gram-negative bacterial outer membranes	129:167	Lipopolysaccharides (LPS) present in the outer leaflet of Gram-negative bacterial outer membranes protect the bacteria from external threats and influence antibiotic permeability as well as immune system recognition.
33600188	2	54	theme	A	311:311	arg1	structure					292:300	The structure	288:300	The structure of lipid A, the anchor of an LPS molecule to the outer membrane,	288:365	The structure of lipid A, the anchor of an LPS molecule to the outer membrane, can make direct influences on membrane properties.
33600188	5	55	theme	distinct	829:836	arg1	structures					846:855	distinct lipid A structures	829:855	distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen	829:896	In this work, five symmetric bilayer systems comprised of distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen were modeled and simulated to explore influences of different lipid A types on membrane properties.
33600188	7	56	theme	peptide	1382:1388	arg1	evasion					1390:1396	cationic antimicrobial peptide evasion	1359:1396	cationic antimicrobial peptide evasion	1359:1396	The modified lipid A is also less negatively charged, which possibly reveals a resistance mechanism to cationic antimicrobial peptide evasion.
33600188	6	57	theme	order	1112:1116	arg1	parameter					1118:1126	acyl chain order parameter	1101:1126	acyl chain order parameter	1101:1126	All-atom molecular dynamics simulations reveal that membrane properties such as hydrophobic thickness, acyl chain order parameter, and area per lipid are largely impacted by lipid A modifications due to differences in composition and acyl chain distortions.
33600188	3	58	theme	structures	556:565	arg1	modifications					531:543	modifications	531:543	modifications of lipid A structures	531:565	Particularly, in Vibrio cholerae, a Gram-negative bacterium responsible for cholera, a severe diarrheal disease, modifications of lipid A structures grant antibiotic resistance and are a primary factor that led to the current cholera pandemic.
33600188	5	59	theme	bilayer	800:806	arg1	systems					808:814	five symmetric bilayer systems	785:814	five symmetric bilayer systems comprised of distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen	785:896	In this work, five symmetric bilayer systems comprised of distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen were modeled and simulated to explore influences of different lipid A types on membrane properties.
33600188	6	60	theme	due	1194:1196	arg1	modifications					1180:1192	lipid A modifications	1172:1192	lipid A modifications due to differences in composition and acyl chain distortions	1172:1253	All-atom molecular dynamics simulations reveal that membrane properties such as hydrophobic thickness, acyl chain order parameter, and area per lipid are largely impacted by lipid A modifications due to differences in composition and acyl chain distortions.
33600188	3	61	theme	lipid	548:552	arg1	structures					556:565	lipid A structures	548:565	lipid A structures	548:565	Particularly, in Vibrio cholerae, a Gram-negative bacterium responsible for cholera, a severe diarrheal disease, modifications of lipid A structures grant antibiotic resistance and are a primary factor that led to the current cholera pandemic.
33600188	1	62	from	threats	204:210	arg1	bacteria					181:188	the bacteria	177:188	the bacteria from external threats	177:210	Lipopolysaccharides (LPS) present in the outer leaflet of Gram-negative bacterial outer membranes protect the bacteria from external threats and influence antibiotic permeability as well as immune system recognition.
33600188	1	63	theme	present	97:103	arg1	LPS					92:94	LPS	92:94	LPS	92:94	Lipopolysaccharides (LPS) present in the outer leaflet of Gram-negative bacterial outer membranes protect the bacteria from external threats and influence antibiotic permeability as well as immune system recognition.
33600188	1	63	theme	present	97:103	arg1	Lipopolysaccharides					71:89	Lipopolysaccharides	71:89	Lipopolysaccharides (LPS) present in the outer leaflet of Gram-negative bacterial outer membranes	71:167	Lipopolysaccharides (LPS) present in the outer leaflet of Gram-negative bacterial outer membranes protect the bacteria from external threats and influence antibiotic permeability as well as immune system recognition.
33600188	0	64	from	Influences	0:9	arg1	Properties					59:68	LPS Bilayer Properties	47:68	LPS Bilayer Properties	47:68	Influences of Vibrio cholerae Lipid A Types on LPS Bilayer Properties.
33600188	6	65	theme	A	1178:1178	arg1	modifications					1180:1192	lipid A modifications	1172:1192	lipid A modifications due to differences in composition and acyl chain distortions	1172:1253	All-atom molecular dynamics simulations reveal that membrane properties such as hydrophobic thickness, acyl chain order parameter, and area per lipid are largely impacted by lipid A modifications due to differences in composition and acyl chain distortions.
33600188	0	66	theme	Bilayer	51:57	arg1	Properties					59:68	LPS Bilayer Properties	47:68	LPS Bilayer Properties	47:68	Influences of Vibrio cholerae Lipid A Types on LPS Bilayer Properties.
33600188	3	67	theme	antibiotic	573:582	arg1	resistance					584:593	antibiotic resistance	573:593	antibiotic resistance	573:593	Particularly, in Vibrio cholerae, a Gram-negative bacterium responsible for cholera, a severe diarrheal disease, modifications of lipid A structures grant antibiotic resistance and are a primary factor that led to the current cholera pandemic.
33600188	5	68	theme	A	844:844	arg1	structures					846:855	distinct lipid A structures	829:855	distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen	829:896	In this work, five symmetric bilayer systems comprised of distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen were modeled and simulated to explore influences of different lipid A types on membrane properties.
33600188	8	69	theme	system	1474:1479	arg1	properties					1489:1498	Vibrio cholerae's immune system evasion properties	1449:1498	Vibrio cholerae's immune system evasion properties	1449:1498	These findings present a possible explanation for Vibrio cholerae's immune system evasion properties and establish the differences between the lipid A types, which should be of use for any future study of the Gram-negative bacteria.
33600188	1	70	theme	Gram-negative	129:141	arg1	membranes					159:167	Gram-negative bacterial outer membranes	129:167	Gram-negative bacterial outer membranes	129:167	Lipopolysaccharides (LPS) present in the outer leaflet of Gram-negative bacterial outer membranes protect the bacteria from external threats and influence antibiotic permeability as well as immune system recognition.
33600188	7	71	theme	cationic	1359:1366	arg1	evasion					1390:1396	cationic antimicrobial peptide evasion	1359:1396	cationic antimicrobial peptide evasion	1359:1396	The modified lipid A is also less negatively charged, which possibly reveals a resistance mechanism to cationic antimicrobial peptide evasion.
33600188	4	72	theme	such	723:726	arg1	modifications					728:740	such modifications	723:740	such modifications	723:740	However, the difference in structural properties incurred by such modifications has not been fully explored.
33600188	6	73	theme	membrane	1050:1057	arg1	thickness					1090:1098	hydrophobic thickness	1078:1098	hydrophobic thickness	1078:1098	All-atom molecular dynamics simulations reveal that membrane properties such as hydrophobic thickness, acyl chain order parameter, and area per lipid are largely impacted by lipid A modifications due to differences in composition and acyl chain distortions.
33600188	6	73	theme	membrane	1050:1057	arg1	properties					1059:1068	membrane properties	1050:1068	membrane properties such as hydrophobic thickness, acyl chain order parameter, and area per lipid	1050:1146	All-atom molecular dynamics simulations reveal that membrane properties such as hydrophobic thickness, acyl chain order parameter, and area per lipid are largely impacted by lipid A modifications due to differences in composition and acyl chain distortions.
33600188	6	73	theme	membrane	1050:1057	arg1	parameter					1118:1126	acyl chain order parameter	1101:1126	acyl chain order parameter	1101:1126	All-atom molecular dynamics simulations reveal that membrane properties such as hydrophobic thickness, acyl chain order parameter, and area per lipid are largely impacted by lipid A modifications due to differences in composition and acyl chain distortions.
33600188	6	73	theme	membrane	1050:1057	arg1	area					1133:1136	area	1133:1136	area per lipid	1133:1146	All-atom molecular dynamics simulations reveal that membrane properties such as hydrophobic thickness, acyl chain order parameter, and area per lipid are largely impacted by lipid A modifications due to differences in composition and acyl chain distortions.
33600188	4	74	theme	structural	689:698	arg1	properties					700:709	structural properties	689:709	structural properties incurred by such modifications	689:740	However, the difference in structural properties incurred by such modifications has not been fully explored.
33600188	0	75	theme	cholerae	21:28	arg1	Types					38:42	Vibrio cholerae Lipid A Types	14:42	Vibrio cholerae Lipid A Types	14:42	Influences of Vibrio cholerae Lipid A Types on LPS Bilayer Properties.
33600188	6	76	theme	dynamics	1017:1024	arg1	simulations					1026:1036	All-atom molecular dynamics simulations	998:1036	All-atom molecular dynamics simulations	998:1036	All-atom molecular dynamics simulations reveal that membrane properties such as hydrophobic thickness, acyl chain order parameter, and area per lipid are largely impacted by lipid A modifications due to differences in composition and acyl chain distortions.
33600188	8	77	theme	A	1548:1548	arg1	types					1550:1554	the lipid A types	1538:1554	the lipid A types	1538:1554	These findings present a possible explanation for Vibrio cholerae's immune system evasion properties and establish the differences between the lipid A types, which should be of use for any future study of the Gram-negative bacteria.
33600188	2	78	theme	direct	376:381	arg1	influences					383:392	direct influences	376:392	direct influences on membrane properties	376:415	The structure of lipid A, the anchor of an LPS molecule to the outer membrane, can make direct influences on membrane properties.
33600188	0	79	theme	A	36:36	arg1	Types					38:42	Vibrio cholerae Lipid A Types	14:42	Vibrio cholerae Lipid A Types	14:42	Influences of Vibrio cholerae Lipid A Types on LPS Bilayer Properties.
33600188	1	80	theme	external	195:202	arg1	threats					204:210	external threats	195:210	external threats	195:210	Lipopolysaccharides (LPS) present in the outer leaflet of Gram-negative bacterial outer membranes protect the bacteria from external threats and influence antibiotic permeability as well as immune system recognition.
33600188	5	81	theme	types	968:972	arg1	influences					936:945	influences	936:945	influences of different lipid A types on membrane properties	936:995	In this work, five symmetric bilayer systems comprised of distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen were modeled and simulated to explore influences of different lipid A types on membrane properties.
33600188	6	82	theme	All-atom	998:1005	arg1	dynamics					1017:1024	All-atom molecular dynamics	998:1024	All-atom molecular dynamics simulations	998:1036	All-atom molecular dynamics simulations reveal that membrane properties such as hydrophobic thickness, acyl chain order parameter, and area per lipid are largely impacted by lipid A modifications due to differences in composition and acyl chain distortions.
33600188	5	83	theme	membrane	977:984	arg1	properties					986:995	membrane properties	977:995	membrane properties	977:995	In this work, five symmetric bilayer systems comprised of distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen were modeled and simulated to explore influences of different lipid A types on membrane properties.
33600188	6	84	theme	chain	1106:1110	arg1	parameter					1118:1126	acyl chain order parameter	1101:1126	acyl chain order parameter	1101:1126	All-atom molecular dynamics simulations reveal that membrane properties such as hydrophobic thickness, acyl chain order parameter, and area per lipid are largely impacted by lipid A modifications due to differences in composition and acyl chain distortions.
33600188	3	85	theme	Gram-negative	454:466	arg1	cholerae					442:449	Vibrio cholerae	435:449	Vibrio cholerae	435:449	Particularly, in Vibrio cholerae, a Gram-negative bacterium responsible for cholera, a severe diarrheal disease, modifications of lipid A structures grant antibiotic resistance and are a primary factor that led to the current cholera pandemic.
33600188	3	85	theme	Gram-negative	454:466	arg1	bacterium					468:476	a Gram-negative bacterium	452:476	a Gram-negative bacterium responsible for cholera, a severe diarrheal disease	452:528	Particularly, in Vibrio cholerae, a Gram-negative bacterium responsible for cholera, a severe diarrheal disease, modifications of lipid A structures grant antibiotic resistance and are a primary factor that led to the current cholera pandemic.
33600188	8	86	theme	Gram-negative	1608:1620	arg1	bacteria					1622:1629	the Gram-negative bacteria	1604:1629	the Gram-negative bacteria	1604:1629	These findings present a possible explanation for Vibrio cholerae's immune system evasion properties and establish the differences between the lipid A types, which should be of use for any future study of the Gram-negative bacteria.
33600188	5	87	theme	lipid	960:964	arg1	types					968:972	different lipid A types	950:972	different lipid A types	950:972	In this work, five symmetric bilayer systems comprised of distinct lipid A structures of Vibrio cholerae LPS with O1 O-antigen were modeled and simulated to explore influences of different lipid A types on membrane properties.
33600188	6	88	theme	hydrophobic	1078:1088	arg1	thickness					1090:1098	hydrophobic thickness	1078:1098	hydrophobic thickness	1078:1098	All-atom molecular dynamics simulations reveal that membrane properties such as hydrophobic thickness, acyl chain order parameter, and area per lipid are largely impacted by lipid A modifications due to differences in composition and acyl chain distortions.
33600188	2	89	theme	molecule	335:342	arg1	anchor					318:323	the anchor	314:323	the anchor of an LPS molecule to the outer membrane	314:364	The structure of lipid A, the anchor of an LPS molecule to the outer membrane, can make direct influences on membrane properties.
33600188	2	89	theme	molecule	335:342	arg1	A					311:311	lipid A	305:311	lipid A	305:311	The structure of lipid A, the anchor of an LPS molecule to the outer membrane, can make direct influences on membrane properties.
32335115	2	0	theme	co-existing	518:528	arg1	anions					530:535	co-existing anions	518:535	co-existing anions	518:535	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	2	0	theme	co-existing	518:528	arg1	parameters					425:434	the influencing parameters	409:434	the influencing parameters	409:434	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	7	1	theme	XPS	1257:1259	arg1	techniques					1261:1270	FTIR, SEM, EDS and XPS techniques	1238:1270	FTIR, SEM, EDS and XPS techniques	1238:1270	Characterization of the adsorbent using FTIR, SEM, EDS and XPS techniques suggested that the possible adsorption mechanisms were electrostatic attraction as well as ligand exchange.
32335115	3	2	theme	Experimental	538:549	arg1	data					551:554	Experimental data	538:554	Experimental data	538:554	Experimental data were well fitted to the Langmuir isotherm model (R2 = 0.9998) as well as the pseudo-second-order model (R2 = 1.000), indicating that the phosphate adsorption process was homogeneous, mono-layered and chemisorption dominated.
32335115	7	3	theme	possible	1291:1298	arg1	mechanisms					1311:1320	the possible adsorption mechanisms	1287:1320	the possible adsorption mechanisms	1287:1320	Characterization of the adsorbent using FTIR, SEM, EDS and XPS techniques suggested that the possible adsorption mechanisms were electrostatic attraction as well as ligand exchange.
32335115	7	3	theme	possible	1291:1298	arg1	attraction					1341:1350	electrostatic attraction	1327:1350	electrostatic attraction as well as ligand exchange	1327:1377	Characterization of the adsorbent using FTIR, SEM, EDS and XPS techniques suggested that the possible adsorption mechanisms were electrostatic attraction as well as ligand exchange.
32335115	1	4	theme	new	278:280	arg1	lanthanum-chitosan					216:233	crosslinked lanthanum-chitosan	204:233	crosslinked lanthanum-chitosan (La-CTS-1X/2X)	204:248	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	1	4	theme	new	278:280	arg1	lanthanum-chitosan					169:186	lanthanum-chitosan	169:186	lanthanum-chitosan	169:186	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	1	4	theme	new	278:280	arg1	biosorbents					290:300	new complex biosorbents	278:300	new complex biosorbents for effective phosphate removal from wastewater	278:348	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	7	5	theme	EDS	1249:1251	arg1	techniques					1261:1270	FTIR, SEM, EDS and XPS techniques	1238:1270	FTIR, SEM, EDS and XPS techniques	1238:1270	Characterization of the adsorbent using FTIR, SEM, EDS and XPS techniques suggested that the possible adsorption mechanisms were electrostatic attraction as well as ligand exchange.
32335115	1	6	link	crosslinked	204:214	arg1	lanthanum-chitosan					216:233	crosslinked lanthanum-chitosan	204:233	crosslinked lanthanum-chitosan (La-CTS-1X/2X)	204:248	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	1	6	link	crosslinked	204:214	arg1	La-CTS-1X/2X					236:247	La-CTS-1X/2X	236:247	La-CTS-1X/2X	236:247	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	1	6	link	crosslinked	204:214	arg1	lanthanum-chitosan					169:186	lanthanum-chitosan	169:186	lanthanum-chitosan	169:186	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	1	6	link	crosslinked	204:214	arg1	biosorbents					290:300	new complex biosorbents	278:300	new complex biosorbents for effective phosphate removal from wastewater	278:348	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	4	7	theme	phosphate	802:810	arg1	47.28					856:860	47.28	856:860	47.28	856:860	Besides, the maximum phosphate adsorption capacity for La-CTS-0X/1X/2X was 47.28, 57.84 and 31.01 mg g-1 at pH 6, respectively.
32335115	4	7	theme	phosphate	802:810	arg1	capacity					823:830	the maximum phosphate adsorption capacity	790:830	the maximum phosphate adsorption capacity for La-CTS-0X/1X/2X	790:850	Besides, the maximum phosphate adsorption capacity for La-CTS-0X/1X/2X was 47.28, 57.84 and 31.01 mg g-1 at pH 6, respectively.
32335115	1	8	theme	complex	282:288	arg1	lanthanum-chitosan					216:233	crosslinked lanthanum-chitosan	204:233	crosslinked lanthanum-chitosan (La-CTS-1X/2X)	204:248	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	1	8	theme	complex	282:288	arg1	lanthanum-chitosan					169:186	lanthanum-chitosan	169:186	lanthanum-chitosan	169:186	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	1	8	theme	complex	282:288	arg1	biosorbents					290:300	new complex biosorbents	278:300	new complex biosorbents for effective phosphate removal from wastewater	278:348	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	1	9	from	wastewater	339:348	arg1	removal					326:332	effective phosphate removal	306:332	effective phosphate removal from wastewater	306:348	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	3	10	theme	Langmuir	580:587	arg1	model					598:602	the Langmuir isotherm model	576:602	the Langmuir isotherm model (R2 = 0.9998) as well as the pseudo-second-order model (R2 = 1.000)	576:670	Experimental data were well fitted to the Langmuir isotherm model (R2 = 0.9998) as well as the pseudo-second-order model (R2 = 1.000), indicating that the phosphate adsorption process was homogeneous, mono-layered and chemisorption dominated.
32335115	3	10	theme	Langmuir	580:587	arg1	R2 = 0.9998					605:615	R2 = 0.9998	605:615	R2 = 0.9998	605:615	Experimental data were well fitted to the Langmuir isotherm model (R2 = 0.9998) as well as the pseudo-second-order model (R2 = 1.000), indicating that the phosphate adsorption process was homogeneous, mono-layered and chemisorption dominated.
32335115	1	11	theme	present	138:144	arg1	study					146:150	the present study	134:150	the present study	134:150	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	0	12	theme	agent	124:128	arg1	amount					100:105	the amount	96:105	the amount of cross-linking agent	96:128	Fast and efficient phosphate removal on lanthanum-chitosan composite synthesized by controlling the amount of cross-linking agent.
32335115	0	12	theme	agent	124:128	arg1	agent					124:128	cross-linking agent	110:128	cross-linking agent	110:128	Fast and efficient phosphate removal on lanthanum-chitosan composite synthesized by controlling the amount of cross-linking agent.
32335115	8	13	dep	met	1496:1498	arg1	limit					1516:1520	the permissible limit	1500:1520	the remained P concentration met the permissible limit	1467:1520	More importantly, the La-CTS-1X had rapid removal rate for phosphate within 10 min and the remained P concentration met the permissible limit by the U.S. Environmental Protection Agency (EPA).
32335115	6	14	theme	regenerated	1090:1100	arg1	materials					1102:1110	The regenerated materials	1086:1110	The regenerated materials	1086:1110	The regenerated materials could be repeatedly used for three cycles without obvious degradation of performance.
32335115	8	15	theme	rapid	1416:1420	arg1	rate					1430:1433	rapid removal rate	1416:1433	rapid removal rate for phosphate	1416:1447	More importantly, the La-CTS-1X had rapid removal rate for phosphate within 10 min and the remained P concentration met the permissible limit by the U.S. Environmental Protection Agency (EPA).
32335115	0	16	theme	cross-linking	110:122	arg1	agent					124:128	cross-linking agent	110:128	cross-linking agent	110:128	Fast and efficient phosphate removal on lanthanum-chitosan composite synthesized by controlling the amount of cross-linking agent.
32335115	4	17	from	pH 6	889:892	arg1	47.28					856:860	47.28	856:860	47.28	856:860	Besides, the maximum phosphate adsorption capacity for La-CTS-0X/1X/2X was 47.28, 57.84 and 31.01 mg g-1 at pH 6, respectively.
32335115	4	17	from	pH 6	889:892	arg1	capacity					823:830	the maximum phosphate adsorption capacity	790:830	the maximum phosphate adsorption capacity for La-CTS-0X/1X/2X	790:850	Besides, the maximum phosphate adsorption capacity for La-CTS-0X/1X/2X was 47.28, 57.84 and 31.01 mg g-1 at pH 6, respectively.
32335115	0	18	theme	Fast	0:3	arg1	removal					29:35	Fast and efficient phosphate removal	0:35	Fast and efficient phosphate removal on lanthanum-chitosan composite	0:67	Fast and efficient phosphate removal on lanthanum-chitosan composite synthesized by controlling the amount of cross-linking agent.
32335115	5	19	theme	Thermodynamic	909:921	arg1	ΔG°					993:995	ΔG°	993:995	ΔG° (-4.60 kJ mol-1)	993:1012	Thermodynamic parameters including ΔH° (-43.7 kJ mol-1), ΔS° (-132 J mol-1 K-1) and ΔG° (-4.60 kJ mol-1) revealed that the essence of adsorption was spontaneous and exothermic.
32335115	5	19	theme	Thermodynamic	909:921	arg1	ΔS°					966:968	ΔS°	966:968	ΔS° (-132 J mol-1 K-1)	966:987	Thermodynamic parameters including ΔH° (-43.7 kJ mol-1), ΔS° (-132 J mol-1 K-1) and ΔG° (-4.60 kJ mol-1) revealed that the essence of adsorption was spontaneous and exothermic.
32335115	5	19	theme	Thermodynamic	909:921	arg1	ΔH°					944:946	ΔH°	944:946	ΔH° (-43.7 kJ mol-1)	944:963	Thermodynamic parameters including ΔH° (-43.7 kJ mol-1), ΔS° (-132 J mol-1 K-1) and ΔG° (-4.60 kJ mol-1) revealed that the essence of adsorption was spontaneous and exothermic.
32335115	5	19	theme	Thermodynamic	909:921	arg1	parameters					923:932	Thermodynamic parameters	909:932	Thermodynamic parameters including ΔH° (-43.7 kJ mol-1), ΔS° (-132 J mol-1 K-1) and ΔG° (-4.60 kJ mol-1)	909:1012	Thermodynamic parameters including ΔH° (-43.7 kJ mol-1), ΔS° (-132 J mol-1 K-1) and ΔG° (-4.60 kJ mol-1) revealed that the essence of adsorption was spontaneous and exothermic.
32335115	7	20	theme	FTIR	1238:1241	arg1	techniques					1261:1270	FTIR, SEM, EDS and XPS techniques	1238:1270	FTIR, SEM, EDS and XPS techniques	1238:1270	Characterization of the adsorbent using FTIR, SEM, EDS and XPS techniques suggested that the possible adsorption mechanisms were electrostatic attraction as well as ligand exchange.
32335115	0	21	from	removal	29:35	arg1	composite					59:67	lanthanum-chitosan composite	40:67	lanthanum-chitosan composite	40:67	Fast and efficient phosphate removal on lanthanum-chitosan composite synthesized by controlling the amount of cross-linking agent.
32335115	2	22	theme	Batch	351:355	arg1	experiments					368:378	Batch adsorption experiments	351:378	Batch adsorption experiments	351:378	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	7	23	theme	electrostatic	1327:1339	arg1	mechanisms					1311:1320	the possible adsorption mechanisms	1287:1320	the possible adsorption mechanisms	1287:1320	Characterization of the adsorbent using FTIR, SEM, EDS and XPS techniques suggested that the possible adsorption mechanisms were electrostatic attraction as well as ligand exchange.
32335115	7	23	theme	electrostatic	1327:1339	arg1	attraction					1341:1350	electrostatic attraction	1327:1350	electrostatic attraction as well as ligand exchange	1327:1377	Characterization of the adsorbent using FTIR, SEM, EDS and XPS techniques suggested that the possible adsorption mechanisms were electrostatic attraction as well as ligand exchange.
32335115	2	24	theme	ions	482:485	arg1	viz.					437:440	viz.	437:440	viz.	437:440	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	2	24	theme	ions	482:485	arg1	pH					443:444	pH	443:444	pH	443:444	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	2	24	theme	ions	482:485	arg1	anions					530:535	co-existing anions	518:535	co-existing anions	518:535	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	2	24	theme	ions	482:485	arg1	concentration					455:467	initial concentration	447:467	initial concentration of phosphate ions	447:485	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	2	24	theme	ions	482:485	arg1	time					496:499	contact time	488:499	contact time	488:499	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	2	24	theme	ions	482:485	arg1	parameters					425:434	the influencing parameters	409:434	the influencing parameters	409:434	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	2	24	theme	ions	482:485	arg1	temperature					502:512	temperature	502:512	temperature	502:512	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	1	25	dep	lanthanum-chitosan	169:186	arg1	composites					250:259	composites	250:259	composites	250:259	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	0	26	theme	lanthanum-chitosan	40:57	arg1	composite					59:67	lanthanum-chitosan composite	40:67	lanthanum-chitosan composite	40:67	Fast and efficient phosphate removal on lanthanum-chitosan composite synthesized by controlling the amount of cross-linking agent.
32335115	2	27	theme	phosphate	472:480	arg1	ions					482:485	phosphate ions	472:485	phosphate ions	472:485	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	1	28	theme	effective	306:314	arg1	removal					326:332	effective phosphate removal	306:332	effective phosphate removal from wastewater	306:348	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	1	29	link	non-crosslinked	153:167	arg1	lanthanum-chitosan					216:233	crosslinked lanthanum-chitosan	204:233	crosslinked lanthanum-chitosan (La-CTS-1X/2X)	204:248	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	1	29	link	non-crosslinked	153:167	arg1	biosorbents					290:300	new complex biosorbents	278:300	new complex biosorbents for effective phosphate removal from wastewater	278:348	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	1	29	link	non-crosslinked	153:167	arg1	La-CTS-0X					189:197	La-CTS-0X	189:197	La-CTS-0X	189:197	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	1	29	link	non-crosslinked	153:167	arg1	lanthanum-chitosan					169:186	lanthanum-chitosan	169:186	lanthanum-chitosan	169:186	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	6	30	used	used	1132:1135	arg2	materials					1102:1110	The regenerated materials	1086:1110	The regenerated materials	1086:1110	The regenerated materials could be repeatedly used for three cycles without obvious degradation of performance.
32335115	3	31	theme	isotherm	589:596	arg1	model					598:602	the Langmuir isotherm model	576:602	the Langmuir isotherm model (R2 = 0.9998) as well as the pseudo-second-order model (R2 = 1.000)	576:670	Experimental data were well fitted to the Langmuir isotherm model (R2 = 0.9998) as well as the pseudo-second-order model (R2 = 1.000), indicating that the phosphate adsorption process was homogeneous, mono-layered and chemisorption dominated.
32335115	3	31	theme	isotherm	589:596	arg1	R2 = 0.9998					605:615	R2 = 0.9998	605:615	R2 = 0.9998	605:615	Experimental data were well fitted to the Langmuir isotherm model (R2 = 0.9998) as well as the pseudo-second-order model (R2 = 1.000), indicating that the phosphate adsorption process was homogeneous, mono-layered and chemisorption dominated.
32335115	1	32	theme	crosslinked	204:214	arg1	lanthanum-chitosan					216:233	crosslinked lanthanum-chitosan	204:233	crosslinked lanthanum-chitosan (La-CTS-1X/2X)	204:248	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	1	32	theme	crosslinked	204:214	arg1	La-CTS-1X/2X					236:247	La-CTS-1X/2X	236:247	La-CTS-1X/2X	236:247	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	1	32	theme	crosslinked	204:214	arg1	lanthanum-chitosan					169:186	lanthanum-chitosan	169:186	lanthanum-chitosan	169:186	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	1	32	theme	crosslinked	204:214	arg1	biosorbents					290:300	new complex biosorbents	278:300	new complex biosorbents for effective phosphate removal from wastewater	278:348	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	0	33	theme	efficient	9:17	arg1	removal					29:35	Fast and efficient phosphate removal	0:35	Fast and efficient phosphate removal on lanthanum-chitosan composite	0:67	Fast and efficient phosphate removal on lanthanum-chitosan composite synthesized by controlling the amount of cross-linking agent.
32335115	1	34	theme	phosphate	316:324	arg1	removal					326:332	effective phosphate removal	306:332	effective phosphate removal from wastewater	306:348	In the present study, non-crosslinked lanthanum-chitosan (La-CTS-0X) and crosslinked lanthanum-chitosan (La-CTS-1X/2X) composites were prepared as new complex biosorbents for effective phosphate removal from wastewater.
32335115	8	35	theme	permissible	1504:1514	arg1	limit					1516:1520	the permissible limit	1500:1520	the remained P concentration met the permissible limit	1467:1520	More importantly, the La-CTS-1X had rapid removal rate for phosphate within 10 min and the remained P concentration met the permissible limit by the U.S. Environmental Protection Agency (EPA).
32335115	2	36	theme	initial	447:453	arg1	parameters					425:434	the influencing parameters	409:434	the influencing parameters	409:434	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	2	36	theme	initial	447:453	arg1	concentration					455:467	initial concentration	447:467	initial concentration of phosphate ions	447:485	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	7	37	theme	adsorption	1300:1309	arg1	mechanisms					1311:1320	the possible adsorption mechanisms	1287:1320	the possible adsorption mechanisms	1287:1320	Characterization of the adsorbent using FTIR, SEM, EDS and XPS techniques suggested that the possible adsorption mechanisms were electrostatic attraction as well as ligand exchange.
32335115	7	37	theme	adsorption	1300:1309	arg1	attraction					1341:1350	electrostatic attraction	1327:1350	electrostatic attraction as well as ligand exchange	1327:1377	Characterization of the adsorbent using FTIR, SEM, EDS and XPS techniques suggested that the possible adsorption mechanisms were electrostatic attraction as well as ligand exchange.
32335115	4	38	theme	maximum	794:800	arg1	47.28					856:860	47.28	856:860	47.28	856:860	Besides, the maximum phosphate adsorption capacity for La-CTS-0X/1X/2X was 47.28, 57.84 and 31.01 mg g-1 at pH 6, respectively.
32335115	4	38	theme	maximum	794:800	arg1	capacity					823:830	the maximum phosphate adsorption capacity	790:830	the maximum phosphate adsorption capacity for La-CTS-0X/1X/2X	790:850	Besides, the maximum phosphate adsorption capacity for La-CTS-0X/1X/2X was 47.28, 57.84 and 31.01 mg g-1 at pH 6, respectively.
32335115	0	39	theme	phosphate	19:27	arg1	removal					29:35	Fast and efficient phosphate removal	0:35	Fast and efficient phosphate removal on lanthanum-chitosan composite	0:67	Fast and efficient phosphate removal on lanthanum-chitosan composite synthesized by controlling the amount of cross-linking agent.
32335115	7	40	theme	adsorbent	1222:1230	arg1	Characterization					1198:1213	Characterization	1198:1213	Characterization of the adsorbent using FTIR, SEM, EDS and XPS techniques	1198:1270	Characterization of the adsorbent using FTIR, SEM, EDS and XPS techniques suggested that the possible adsorption mechanisms were electrostatic attraction as well as ligand exchange.
32335115	8	41	theme	U.S.	1529:1532	arg1	EPA					1567:1569	EPA	1567:1569	EPA	1567:1569	More importantly, the La-CTS-1X had rapid removal rate for phosphate within 10 min and the remained P concentration met the permissible limit by the U.S. Environmental Protection Agency (EPA).
32335115	8	41	theme	U.S.	1529:1532	arg1	Agency					1559:1564	the U.S. Environmental Protection Agency	1525:1564	the U.S. Environmental Protection Agency (EPA)	1525:1570	More importantly, the La-CTS-1X had rapid removal rate for phosphate within 10 min and the remained P concentration met the permissible limit by the U.S. Environmental Protection Agency (EPA).
32335115	8	42	contain	had	1412:1414	arg2	rate					1430:1433	rapid removal rate	1416:1433	rapid removal rate for phosphate	1416:1447	More importantly, the La-CTS-1X had rapid removal rate for phosphate within 10 min and the remained P concentration met the permissible limit by the U.S. Environmental Protection Agency (EPA).
32335115	8	42	contain	had	1412:1414	arg1	La-CTS-1X					1402:1410	the La-CTS-1X	1398:1410	the La-CTS-1X	1398:1410	More importantly, the La-CTS-1X had rapid removal rate for phosphate within 10 min and the remained P concentration met the permissible limit by the U.S. Environmental Protection Agency (EPA).
32335115	8	43	theme	removal	1422:1428	arg1	rate					1430:1433	rapid removal rate	1416:1433	rapid removal rate for phosphate	1416:1447	More importantly, the La-CTS-1X had rapid removal rate for phosphate within 10 min and the remained P concentration met the permissible limit by the U.S. Environmental Protection Agency (EPA).
32335115	6	44	theme	performance	1185:1195	arg1	degradation					1170:1180	obvious degradation	1162:1180	obvious degradation of performance	1162:1195	The regenerated materials could be repeatedly used for three cycles without obvious degradation of performance.
32335115	2	45	theme	contact	488:494	arg1	time					496:499	contact time	488:499	contact time	488:499	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	2	45	theme	contact	488:494	arg1	parameters					425:434	the influencing parameters	409:434	the influencing parameters	409:434	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	3	46	theme	phosphate	693:701	arg1	homogeneous					726:736	homogeneous	726:736	homogeneous	726:736	Experimental data were well fitted to the Langmuir isotherm model (R2 = 0.9998) as well as the pseudo-second-order model (R2 = 1.000), indicating that the phosphate adsorption process was homogeneous, mono-layered and chemisorption dominated.
32335115	3	46	theme	phosphate	693:701	arg1	process					714:720	the phosphate adsorption process	689:720	the phosphate adsorption process	689:720	Experimental data were well fitted to the Langmuir isotherm model (R2 = 0.9998) as well as the pseudo-second-order model (R2 = 1.000), indicating that the phosphate adsorption process was homogeneous, mono-layered and chemisorption dominated.
32335115	8	47	theme	concentration	1482:1494	arg1	met					1496:1498	the remained P concentration met	1467:1498	the remained P concentration met the permissible limit	1467:1520	More importantly, the La-CTS-1X had rapid removal rate for phosphate within 10 min and the remained P concentration met the permissible limit by the U.S. Environmental Protection Agency (EPA).
32335115	5	48	theme	adsorption	1043:1052	arg1	spontaneous					1058:1068	spontaneous	1058:1068	spontaneous	1058:1068	Thermodynamic parameters including ΔH° (-43.7 kJ mol-1), ΔS° (-132 J mol-1 K-1) and ΔG° (-4.60 kJ mol-1) revealed that the essence of adsorption was spontaneous and exothermic.
32335115	5	48	theme	adsorption	1043:1052	arg1	essence					1032:1038	the essence	1028:1038	the essence of adsorption	1028:1052	Thermodynamic parameters including ΔH° (-43.7 kJ mol-1), ΔS° (-132 J mol-1 K-1) and ΔG° (-4.60 kJ mol-1) revealed that the essence of adsorption was spontaneous and exothermic.
32335115	3	49	theme	adsorption	703:712	arg1	homogeneous					726:736	homogeneous	726:736	homogeneous	726:736	Experimental data were well fitted to the Langmuir isotherm model (R2 = 0.9998) as well as the pseudo-second-order model (R2 = 1.000), indicating that the phosphate adsorption process was homogeneous, mono-layered and chemisorption dominated.
32335115	3	49	theme	adsorption	703:712	arg1	process					714:720	the phosphate adsorption process	689:720	the phosphate adsorption process	689:720	Experimental data were well fitted to the Langmuir isotherm model (R2 = 0.9998) as well as the pseudo-second-order model (R2 = 1.000), indicating that the phosphate adsorption process was homogeneous, mono-layered and chemisorption dominated.
32335115	8	50	theme	P	1480:1480	arg1	met					1496:1498	the remained P concentration met	1467:1498	the remained P concentration met the permissible limit	1467:1520	More importantly, the La-CTS-1X had rapid removal rate for phosphate within 10 min and the remained P concentration met the permissible limit by the U.S. Environmental Protection Agency (EPA).
32335115	6	51	theme	obvious	1162:1168	arg1	degradation					1170:1180	obvious degradation	1162:1180	obvious degradation of performance	1162:1195	The regenerated materials could be repeatedly used for three cycles without obvious degradation of performance.
32335115	4	52	theme	adsorption	812:821	arg1	47.28					856:860	47.28	856:860	47.28	856:860	Besides, the maximum phosphate adsorption capacity for La-CTS-0X/1X/2X was 47.28, 57.84 and 31.01 mg g-1 at pH 6, respectively.
32335115	4	52	theme	adsorption	812:821	arg1	capacity					823:830	the maximum phosphate adsorption capacity	790:830	the maximum phosphate adsorption capacity for La-CTS-0X/1X/2X	790:850	Besides, the maximum phosphate adsorption capacity for La-CTS-0X/1X/2X was 47.28, 57.84 and 31.01 mg g-1 at pH 6, respectively.
32335115	8	53	theme	Protection	1548:1557	arg1	EPA					1567:1569	EPA	1567:1569	EPA	1567:1569	More importantly, the La-CTS-1X had rapid removal rate for phosphate within 10 min and the remained P concentration met the permissible limit by the U.S. Environmental Protection Agency (EPA).
32335115	8	53	theme	Protection	1548:1557	arg1	Agency					1559:1564	the U.S. Environmental Protection Agency	1525:1564	the U.S. Environmental Protection Agency (EPA)	1525:1570	More importantly, the La-CTS-1X had rapid removal rate for phosphate within 10 min and the remained P concentration met the permissible limit by the U.S. Environmental Protection Agency (EPA).
32335115	8	54	theme	remained	1471:1478	arg1	met					1496:1498	the remained P concentration met	1467:1498	the remained P concentration met the permissible limit	1467:1520	More importantly, the La-CTS-1X had rapid removal rate for phosphate within 10 min and the remained P concentration met the permissible limit by the U.S. Environmental Protection Agency (EPA).
32335115	2	55	theme	adsorption	357:366	arg1	experiments					368:378	Batch adsorption experiments	351:378	Batch adsorption experiments	351:378	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	8	56	theme	Environmental	1534:1546	arg1	EPA					1567:1569	EPA	1567:1569	EPA	1567:1569	More importantly, the La-CTS-1X had rapid removal rate for phosphate within 10 min and the remained P concentration met the permissible limit by the U.S. Environmental Protection Agency (EPA).
32335115	8	56	theme	Environmental	1534:1546	arg1	Agency					1559:1564	the U.S. Environmental Protection Agency	1525:1564	the U.S. Environmental Protection Agency (EPA)	1525:1570	More importantly, the La-CTS-1X had rapid removal rate for phosphate within 10 min and the remained P concentration met the permissible limit by the U.S. Environmental Protection Agency (EPA).
32335115	7	57	theme	ligand	1363:1368	arg1	exchange					1370:1377	ligand exchange	1363:1377	electrostatic attraction as well as ligand exchange	1327:1377	Characterization of the adsorbent using FTIR, SEM, EDS and XPS techniques suggested that the possible adsorption mechanisms were electrostatic attraction as well as ligand exchange.
32335115	2	58	theme	influencing	413:423	arg1	viz.					437:440	viz.	437:440	viz.	437:440	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	2	58	theme	influencing	413:423	arg1	pH					443:444	pH	443:444	pH	443:444	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	2	58	theme	influencing	413:423	arg1	anions					530:535	co-existing anions	518:535	co-existing anions	518:535	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	2	58	theme	influencing	413:423	arg1	concentration					455:467	initial concentration	447:467	initial concentration of phosphate ions	447:485	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	2	58	theme	influencing	413:423	arg1	time					496:499	contact time	488:499	contact time	488:499	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	2	58	theme	influencing	413:423	arg1	parameters					425:434	the influencing parameters	409:434	the influencing parameters	409:434	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	2	58	theme	influencing	413:423	arg1	temperature					502:512	temperature	502:512	temperature	502:512	Batch adsorption experiments were investigated by varying the influencing parameters, viz., pH, initial concentration of phosphate ions, contact time, temperature and co-existing anions.
32335115	7	59	theme	SEM	1244:1246	arg1	techniques					1261:1270	FTIR, SEM, EDS and XPS techniques	1238:1270	FTIR, SEM, EDS and XPS techniques	1238:1270	Characterization of the adsorbent using FTIR, SEM, EDS and XPS techniques suggested that the possible adsorption mechanisms were electrostatic attraction as well as ligand exchange.
32335115	3	60	theme	pseudo-second-order	633:651	arg1	R2 = 1.000					660:669	R2 = 1.000	660:669	R2 = 1.000	660:669	Experimental data were well fitted to the Langmuir isotherm model (R2 = 0.9998) as well as the pseudo-second-order model (R2 = 1.000), indicating that the phosphate adsorption process was homogeneous, mono-layered and chemisorption dominated.
32335115	3	60	theme	pseudo-second-order	633:651	arg1	model					653:657	the pseudo-second-order model	629:657	the Langmuir isotherm model (R2 = 0.9998) as well as the pseudo-second-order model (R2 = 1.000)	576:670	Experimental data were well fitted to the Langmuir isotherm model (R2 = 0.9998) as well as the pseudo-second-order model (R2 = 1.000), indicating that the phosphate adsorption process was homogeneous, mono-layered and chemisorption dominated.
34742823	7	0	theme	secondary	1961:1969	arg1	wastewater					1979:1988	secondary treated wastewater	1961:1988	secondary treated wastewater	1961:1988	In addition, we have also demonstrated the performance of β-CD-EDTA-CS for the of four heavy metals Hg2+, Cd2+, Ni2+, and Cu2+ and three dyes MB, CV, and SO in secondary treated wastewater.
34742823	4	1	dep	mercury	1060:1066	arg1	i.e.					1054:1057	i.e.	1054:1057	i.e.	1054:1057	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	8	2	theme	wastewater	2090:2099	arg1	treatment					2101:2109	wastewater treatment	2090:2109	wastewater treatment	2090:2109	Findings of this study indicate that β-CD-EDTA-CS simple and essay to synthesize and can be use in wastewater treatment.
34742823	4	3	theme	adsorption	1262:1271	arg1	capacity					1273:1280	the adsorption capacity	1258:1280	the adsorption capacity of β-CD-EDTA-CS in aqueous solution	1258:1316	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	4	4	theme	blue	1135:1138	arg1	violet					1154:1159	crystal violet	1146:1159	crystal violet (CV)	1146:1164	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	4	4	theme	blue	1135:1138	arg1	O					1179:1179	safranin O	1170:1179	safranin O	1170:1179	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	4	4	theme	blue	1135:1138	arg1	methylene					1125:1133	methylene blue	1125:1138	methylene blue (MB)	1125:1143	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	4	4	theme	blue	1135:1138	arg1	MB					1141:1142	MB	1141:1142	MB	1141:1142	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	3	5	theme	energy	792:797	arg1	diffraction					702:712	powder X-ray diffraction	689:712	powder X-ray diffraction	689:712	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	3	5	theme	energy	792:797	arg1	spectroscopy					810:821	energy dispersive spectroscopy	792:821	energy dispersive spectroscopy	792:821	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	2	6	theme	simple	356:361	arg1	cross-linker					438:449	a cross-linker	436:449	a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc	436:636	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	2	6	theme	simple	356:361	arg1	route					372:376	an easy and simple chemical route	344:376	an easy and simple chemical route by the reaction of activated β-CD with CS	344:418	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	1	7	theme	water	202:206	arg1	pollution					208:216	water pollution	202:216	water pollution	202:216	Heavy metals and organic dyes are the major source of water pollution.
34742823	2	8	theme	easy	347:350	arg1	cross-linker					438:449	a cross-linker	436:449	a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc	436:636	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	2	8	theme	easy	347:350	arg1	route					372:376	an easy and simple chemical route	344:376	an easy and simple chemical route by the reaction of activated β-CD with CS	344:418	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	6	9	theme	higher	1674:1679	arg1	0.00161-0.00368 g mg-1 min-1					1704:1731	0.00161-0.00368 g mg-1 min-1	1704:1731	0.00161-0.00368 g mg-1 min-1	1704:1731	Kinetics results followed pseudo-second order (PSO) kinetics behavior for both metal ions and dyes, and higher rate constants values (0.00161-0.00368 g mg-1 min-1) for dyes confirmed the cavitation of organic dyes (physisorption).
34742823	6	9	theme	higher	1674:1679	arg1	values					1696:1701	higher rate constants values	1674:1701	higher rate constants values (0.00161-0.00368 g mg-1 min-1) for dyes	1674:1741	Kinetics results followed pseudo-second order (PSO) kinetics behavior for both metal ions and dyes, and higher rate constants values (0.00161-0.00368 g mg-1 min-1) for dyes confirmed the cavitation of organic dyes (physisorption).
34742823	2	10	theme	β-cyclodextrin-ethylenediaminetetraacetic	243:283	arg1	polymer					314:320	a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer	227:320	a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer	227:320	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	6	11	theme	pseudo-second	1596:1608	arg1	behavior					1631:1638	pseudo-second order (PSO) kinetics behavior	1596:1638	pseudo-second order (PSO) kinetics behavior for both metal ions and dyes	1596:1667	Kinetics results followed pseudo-second order (PSO) kinetics behavior for both metal ions and dyes, and higher rate constants values (0.00161-0.00368 g mg-1 min-1) for dyes confirmed the cavitation of organic dyes (physisorption).
34742823	2	12	theme	initial	598:604	arg1	pH					581:582	pH	581:582	pH	581:582	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	2	12	theme	initial	598:604	arg1	concentration					606:618	initial concentration	598:618	initial concentration	598:618	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	1	13	theme	Heavy	148:152	arg1	metals					154:159	Heavy metals	148:159	Heavy metals	148:159	Heavy metals and organic dyes are the major source of water pollution.
34742823	1	13	theme	Heavy	148:152	arg1	dyes					173:176	organic dyes	165:176	organic dyes	165:176	Heavy metals and organic dyes are the major source of water pollution.
34742823	1	13	theme	Heavy	148:152	arg1	source					192:197	the major source	182:197	the major source of water pollution	182:216	Heavy metals and organic dyes are the major source of water pollution.
34742823	6	14	dep	higher	1674:1679	arg1	constants					1686:1694	rate constants	1681:1694	rate constants	1681:1694	Kinetics results followed pseudo-second order (PSO) kinetics behavior for both metal ions and dyes, and higher rate constants values (0.00161-0.00368 g mg-1 min-1) for dyes confirmed the cavitation of organic dyes (physisorption).
34742823	2	15	from	solution	537:544	arg1	removal					480:486	the removal	476:486	the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc	476:636	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	7	16	from	performance	1844:1854	arg1	wastewater					1979:1988	secondary treated wastewater	1961:1988	secondary treated wastewater	1961:1988	In addition, we have also demonstrated the performance of β-CD-EDTA-CS for the of four heavy metals Hg2+, Cd2+, Ni2+, and Cu2+ and three dyes MB, CV, and SO in secondary treated wastewater.
34742823	2	17	theme	aqueous	529:535	arg1	solution					537:544	aqueous solution	529:544	aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc	529:636	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	6	18	theme	metal	1649:1653	arg1	ions					1655:1658	metal ions	1649:1658	metal ions	1649:1658	Kinetics results followed pseudo-second order (PSO) kinetics behavior for both metal ions and dyes, and higher rate constants values (0.00161-0.00368 g mg-1 min-1) for dyes confirmed the cavitation of organic dyes (physisorption).
34742823	2	19	theme	pollutants	513:522	arg1	removal					480:486	the removal	476:486	the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc	476:636	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	4	20	from	solution	1309:1316	arg1	capacity					1273:1280	the adsorption capacity	1258:1280	the adsorption capacity of β-CD-EDTA-CS in aqueous solution	1258:1316	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	3	21	theme	scanning	762:769	arg1	microscopy					780:789	field scanning electron microscopy	756:789	field scanning electron microscopy	756:789	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	3	21	theme	scanning	762:769	arg1	diffraction					702:712	powder X-ray diffraction	689:712	powder X-ray diffraction	689:712	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	0	22	theme	metals	107:112	arg1	removal					90:96	simultaneous removal	77:96	simultaneous removal of heavy metals and organic dyes from wastewater	77:145	Multifunctional β-Cyclodextrin-EDTA-Chitosan polymer adsorbent synthesis for simultaneous removal of heavy metals and organic dyes from wastewater.
34742823	3	23	theme	analyzer	872:879	arg1	techniques					881:890	thermogravimetric analyzer techniques	854:890	thermogravimetric analyzer techniques	854:890	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	0	24	theme	organic	118:124	arg1	dyes					126:129	organic dyes	118:129	organic dyes	118:129	Multifunctional β-Cyclodextrin-EDTA-Chitosan polymer adsorbent synthesis for simultaneous removal of heavy metals and organic dyes from wastewater.
34742823	3	25	theme	surface	976:982	arg1	compositions					962:973	their structural, functional, morphological, elemental compositions	907:973	their structural, functional, morphological, elemental compositions	907:973	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	3	25	theme	surface	976:982	arg1	area					984:987	surface area	976:987	surface area	976:987	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	4	26	theme	heavy	1040:1044	arg1	metals					1046:1051	heavy metals	1040:1051	heavy metals	1040:1051	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	0	27	theme	Multifunctional	0:14	arg1	polymer					45:51	Multifunctional β-Cyclodextrin-EDTA-Chitosan polymer	0:51	Multifunctional β-Cyclodextrin-EDTA-Chitosan polymer	0:51	Multifunctional β-Cyclodextrin-EDTA-Chitosan polymer adsorbent synthesis for simultaneous removal of heavy metals and organic dyes from wastewater.
34742823	3	28	dep	Fourier	715:721	arg1	transform					723:731	transform	723:731	transform infrared spectroscopy	723:753	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	2	29	theme	amidation	452:460	arg1	cross-linker					438:449	a cross-linker	436:449	a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc	436:636	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	2	29	theme	amidation	452:460	arg1	reaction					462:469	amidation reaction	452:469	amidation reaction	452:469	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	6	30	theme	dyes	1779:1782	arg1	cavitation					1757:1766	the cavitation	1753:1766	the cavitation of organic dyes (physisorption)	1753:1798	Kinetics results followed pseudo-second order (PSO) kinetics behavior for both metal ions and dyes, and higher rate constants values (0.00161-0.00368 g mg-1 min-1) for dyes confirmed the cavitation of organic dyes (physisorption).
34742823	7	31	dep	metals	1894:1899	arg1	Ni2+					1913:1916	Ni2+	1913:1916	Ni2+	1913:1916	In addition, we have also demonstrated the performance of β-CD-EDTA-CS for the of four heavy metals Hg2+, Cd2+, Ni2+, and Cu2+ and three dyes MB, CV, and SO in secondary treated wastewater.
34742823	7	31	dep	metals	1894:1899	arg1	metals					1894:1899	four heavy metals	1883:1899	four heavy metals Hg2+, Cd2+, Ni2+, and Cu2+	1883:1926	In addition, we have also demonstrated the performance of β-CD-EDTA-CS for the of four heavy metals Hg2+, Cd2+, Ni2+, and Cu2+ and three dyes MB, CV, and SO in secondary treated wastewater.
34742823	7	31	dep	metals	1894:1899	arg1	CV					1947:1948	CV	1947:1948	CV	1947:1948	In addition, we have also demonstrated the performance of β-CD-EDTA-CS for the of four heavy metals Hg2+, Cd2+, Ni2+, and Cu2+ and three dyes MB, CV, and SO in secondary treated wastewater.
34742823	7	31	dep	metals	1894:1899	arg1	Hg2+					1901:1904	Hg2+	1901:1904	Hg2+	1901:1904	In addition, we have also demonstrated the performance of β-CD-EDTA-CS for the of four heavy metals Hg2+, Cd2+, Ni2+, and Cu2+ and three dyes MB, CV, and SO in secondary treated wastewater.
34742823	7	31	dep	metals	1894:1899	arg1	Cu2+					1923:1926	Cu2+	1923:1926	Cu2+	1923:1926	In addition, we have also demonstrated the performance of β-CD-EDTA-CS for the of four heavy metals Hg2+, Cd2+, Ni2+, and Cu2+ and three dyes MB, CV, and SO in secondary treated wastewater.
34742823	7	31	dep	metals	1894:1899	arg1	Cd2+					1907:1910	Cd2+	1907:1910	Cd2+	1907:1910	In addition, we have also demonstrated the performance of β-CD-EDTA-CS for the of four heavy metals Hg2+, Cd2+, Ni2+, and Cu2+ and three dyes MB, CV, and SO in secondary treated wastewater.
34742823	2	32	with	reaction	385:392	arg1	CS					417:418	CS	417:418	CS	417:418	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	5	33	theme	adsorption	1352:1361	arg1	capacity					1363:1370	monolayer adsorption capacity 346.30 ± 14.0	1342:1384	monolayer adsorption capacity 346.30 ± 14.0	1342:1384	The β-CD-EDTA-CS shows monolayer adsorption capacity 346.30 ± 14.0 and 202.90 ± 13.90 mg g-1 for Hg2+ and Cd2+, respectively, and a heterogeneous adsorption capacity 107.20 ± 5.70, 77.40 ± 5.30 and 55.30 ± 3.60 mg g-1 for MB, CV and SO, respectively.
34742823	0	34	theme	simultaneous	77:88	arg1	removal					90:96	simultaneous removal	77:96	simultaneous removal of heavy metals and organic dyes from wastewater	77:145	Multifunctional β-Cyclodextrin-EDTA-Chitosan polymer adsorbent synthesis for simultaneous removal of heavy metals and organic dyes from wastewater.
34742823	7	35	theme	β-CD-EDTA-CS	1859:1870	arg1	performance					1844:1854	the performance	1840:1854	the performance of β-CD-EDTA-CS for the of four heavy metals Hg2+, Cd2+, Ni2+, and Cu2+ and three dyes MB, CV, and SO in secondary treated wastewater	1840:1988	In addition, we have also demonstrated the performance of β-CD-EDTA-CS for the of four heavy metals Hg2+, Cd2+, Ni2+, and Cu2+ and three dyes MB, CV, and SO in secondary treated wastewater.
34742823	3	36	theme	X-ray	696:700	arg1	Brunauer-Emmett-Teller					824:845	Brunauer-Emmett-Teller	824:845	Brunauer-Emmett-Teller (BET)	824:851	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	3	36	theme	X-ray	696:700	arg1	microscopy					780:789	field scanning electron microscopy	756:789	field scanning electron microscopy	756:789	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	3	36	theme	X-ray	696:700	arg1	diffraction					702:712	powder X-ray diffraction	689:712	powder X-ray diffraction	689:712	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	3	36	theme	X-ray	696:700	arg1	Fourier					715:721	Fourier	715:721	Fourier transform infrared spectroscopy	715:753	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	3	36	theme	X-ray	696:700	arg1	spectroscopy					810:821	energy dispersive spectroscopy	792:821	energy dispersive spectroscopy	792:821	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	4	37	theme	β-CD-EDTA-CS	1285:1296	arg1	capacity					1273:1280	the adsorption capacity	1258:1280	the adsorption capacity of β-CD-EDTA-CS in aqueous solution	1258:1316	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	2	38	theme	activated	397:405	arg1	β-CD					407:410	activated β-CD	397:410	activated β-CD	397:410	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	7	39	theme	treated	1971:1977	arg1	wastewater					1979:1988	secondary treated wastewater	1961:1988	secondary treated wastewater	1961:1988	In addition, we have also demonstrated the performance of β-CD-EDTA-CS for the of four heavy metals Hg2+, Cd2+, Ni2+, and Cu2+ and three dyes MB, CV, and SO in secondary treated wastewater.
34742823	5	40	theme	adsorption	1465:1474	arg1	capacity					1476:1483	a heterogeneous adsorption capacity 107.20 ± 5.70	1449:1497	a heterogeneous adsorption capacity 107.20 ± 5.70	1449:1497	The β-CD-EDTA-CS shows monolayer adsorption capacity 346.30 ± 14.0 and 202.90 ± 13.90 mg g-1 for Hg2+ and Cd2+, respectively, and a heterogeneous adsorption capacity 107.20 ± 5.70, 77.40 ± 5.30 and 55.30 ± 3.60 mg g-1 for MB, CV and SO, respectively.
34742823	4	41	theme	aqueous	1301:1307	arg1	solution					1309:1316	aqueous solution	1301:1316	aqueous solution	1301:1316	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	4	42	theme	metals	1046:1051	arg1	pollutants					1223:1232	inorganic and organic pollutants	1201:1232	inorganic and organic pollutants	1201:1232	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	4	42	theme	metals	1046:1051	arg1	types					1031:1035	Two types	1027:1035	Two types	1027:1035	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	4	42	theme	metals	1046:1051	arg1	dyes					1113:1116	three organic dyes	1099:1116	three organic dyes	1099:1116	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	3	43	theme	electron	771:778	arg1	microscopy					780:789	field scanning electron microscopy	756:789	field scanning electron microscopy	756:789	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	3	43	theme	electron	771:778	arg1	diffraction					702:712	powder X-ray diffraction	689:712	powder X-ray diffraction	689:712	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	4	44	dep	violet	1154:1159	arg1	SO					1182:1183	SO	1182:1183	SO	1182:1183	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	1	45	theme	major	186:190	arg1	metals					154:159	Heavy metals	148:159	Heavy metals	148:159	Heavy metals and organic dyes are the major source of water pollution.
34742823	1	45	theme	major	186:190	arg1	dyes					173:176	organic dyes	165:176	organic dyes	165:176	Heavy metals and organic dyes are the major source of water pollution.
34742823	1	45	theme	major	186:190	arg1	source					192:197	the major source	182:197	the major source of water pollution	182:216	Heavy metals and organic dyes are the major source of water pollution.
34742823	8	46	from	use	2083:2085	arg1	treatment					2101:2109	wastewater treatment	2090:2109	wastewater treatment	2090:2109	Findings of this study indicate that β-CD-EDTA-CS simple and essay to synthesize and can be use in wastewater treatment.
34742823	3	47	theme	field	756:760	arg1	microscopy					780:789	field scanning electron microscopy	756:789	field scanning electron microscopy	756:789	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	3	47	theme	field	756:760	arg1	diffraction					702:712	powder X-ray diffraction	689:712	powder X-ray diffraction	689:712	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	2	48	theme	chemical	363:370	arg1	cross-linker					438:449	a cross-linker	436:449	a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc	436:636	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	2	48	theme	chemical	363:370	arg1	route					372:376	an easy and simple chemical route	344:376	an easy and simple chemical route by the reaction of activated β-CD with CS	344:418	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	3	49	theme	dispersive	799:808	arg1	diffraction					702:712	powder X-ray diffraction	689:712	powder X-ray diffraction	689:712	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	3	49	theme	dispersive	799:808	arg1	spectroscopy					810:821	energy dispersive spectroscopy	792:821	energy dispersive spectroscopy	792:821	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	1	50	theme	pollution	208:216	arg1	metals					154:159	Heavy metals	148:159	Heavy metals	148:159	Heavy metals and organic dyes are the major source of water pollution.
34742823	1	50	theme	pollution	208:216	arg1	dyes					173:176	organic dyes	165:176	organic dyes	165:176	Heavy metals and organic dyes are the major source of water pollution.
34742823	1	50	theme	pollution	208:216	arg1	source					192:197	the major source	182:197	the major source of water pollution	182:216	Heavy metals and organic dyes are the major source of water pollution.
34742823	6	51	theme	rate	1681:1684	arg1	constants					1686:1694	rate constants	1681:1694	rate constants	1681:1694	Kinetics results followed pseudo-second order (PSO) kinetics behavior for both metal ions and dyes, and higher rate constants values (0.00161-0.00368 g mg-1 min-1) for dyes confirmed the cavitation of organic dyes (physisorption).
34742823	2	52	theme	acid-chitosan	285:297	arg1	polymer					314:320	a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer	227:320	a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer	227:320	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	2	53	theme	trifunctional	229:241	arg1	polymer					314:320	a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer	227:320	a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer	227:320	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	0	54	from	wastewater	136:145	arg1	removal					90:96	simultaneous removal	77:96	simultaneous removal of heavy metals and organic dyes from wastewater	77:145	Multifunctional β-Cyclodextrin-EDTA-Chitosan polymer adsorbent synthesis for simultaneous removal of heavy metals and organic dyes from wastewater.
34742823	4	55	theme	safranin	1170:1177	arg1	O					1179:1179	safranin O	1170:1179	safranin O	1170:1179	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	4	55	theme	safranin	1170:1177	arg1	methylene					1125:1133	methylene blue	1125:1138	methylene blue (MB)	1125:1143	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	6	56	theme	Kinetics	1570:1577	arg1	results					1579:1585	Kinetics results	1570:1585	Kinetics results	1570:1585	Kinetics results followed pseudo-second order (PSO) kinetics behavior for both metal ions and dyes, and higher rate constants values (0.00161-0.00368 g mg-1 min-1) for dyes confirmed the cavitation of organic dyes (physisorption).
34742823	2	57	theme	time	585:588	arg1	pH					581:582	pH	581:582	pH	581:582	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	2	57	theme	time	585:588	arg1	effect					590:595	time effect	585:595	time effect	585:595	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	4	58	theme	crystal	1146:1152	arg1	violet					1154:1159	crystal violet	1146:1159	crystal violet (CV)	1146:1164	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	4	58	theme	crystal	1146:1152	arg1	methylene					1125:1133	methylene blue	1125:1138	methylene blue (MB)	1125:1143	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	4	58	theme	crystal	1146:1152	arg1	CV					1162:1163	CV	1162:1163	CV	1162:1163	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	3	59	theme	thermogravimetric	854:870	arg1	techniques					881:890	thermogravimetric analyzer techniques	854:890	thermogravimetric analyzer techniques	854:890	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	0	60	theme	heavy	101:105	arg1	metals					107:112	heavy metals	101:112	heavy metals	101:112	Multifunctional β-Cyclodextrin-EDTA-Chitosan polymer adsorbent synthesis for simultaneous removal of heavy metals and organic dyes from wastewater.
34742823	2	61	theme	β-CD-EDTA-CS	300:311	arg1	polymer					314:320	a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer	227:320	a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer	227:320	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	1	62	theme	organic	165:171	arg1	metals					154:159	Heavy metals	148:159	Heavy metals	148:159	Heavy metals and organic dyes are the major source of water pollution.
34742823	1	62	theme	organic	165:171	arg1	dyes					173:176	organic dyes	165:176	organic dyes	165:176	Heavy metals and organic dyes are the major source of water pollution.
34742823	1	62	theme	organic	165:171	arg1	source					192:197	the major source	182:197	the major source of water pollution	182:216	Heavy metals and organic dyes are the major source of water pollution.
34742823	3	63	theme	structural	913:922	arg1	compositions					962:973	their structural, functional, morphological, elemental compositions	907:973	their structural, functional, morphological, elemental compositions	907:973	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	3	63	theme	structural	913:922	arg1	area					984:987	surface area	976:987	surface area	976:987	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	3	63	theme	structural	913:922	arg1	properties					1001:1010	thermal properties	993:1010	thermal properties	993:1010	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	2	64	theme	organic	505:511	arg1	pollutants					513:522	inorganic and organic pollutants	491:522	inorganic and organic pollutants	491:522	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	0	65	theme	dyes	126:129	arg1	removal					90:96	simultaneous removal	77:96	simultaneous removal of heavy metals and organic dyes from wastewater	77:145	Multifunctional β-Cyclodextrin-EDTA-Chitosan polymer adsorbent synthesis for simultaneous removal of heavy metals and organic dyes from wastewater.
34742823	3	66	dep	structural	913:922	arg1	morphological					937:949	morphological	937:949	morphological	937:949	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	3	66	dep	structural	913:922	arg1	functional					925:934	functional	925:934	functional	925:934	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	3	66	dep	structural	913:922	arg1	elemental					952:960	elemental	952:960	elemental	952:960	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	2	67	theme	different	552:560	arg1	pH					581:582	pH	581:582	pH	581:582	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	2	67	theme	different	552:560	arg1	parameters					562:571	different parameters	552:571	different parameters such as pH, time effect, initial concentration, reusability, etc	552:636	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	4	68	from	β-CD-EDTA-CS	1285:1296	arg1	solution					1309:1316	aqueous solution	1301:1316	aqueous solution	1301:1316	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	4	69	theme	inorganic	1201:1209	arg1	pollutants					1223:1232	inorganic and organic pollutants	1201:1232	inorganic and organic pollutants	1201:1232	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	4	69	theme	inorganic	1201:1209	arg1	types					1031:1035	Two types	1027:1035	Two types	1027:1035	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	4	69	theme	inorganic	1201:1209	arg1	dyes					1113:1116	three organic dyes	1099:1116	three organic dyes	1099:1116	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	6	70	theme	kinetics	1622:1629	arg1	behavior					1631:1638	pseudo-second order (PSO) kinetics behavior	1596:1638	pseudo-second order (PSO) kinetics behavior for both metal ions and dyes	1596:1667	Kinetics results followed pseudo-second order (PSO) kinetics behavior for both metal ions and dyes, and higher rate constants values (0.00161-0.00368 g mg-1 min-1) for dyes confirmed the cavitation of organic dyes (physisorption).
34742823	4	71	from	capacity	1273:1280	arg1	solution					1309:1316	aqueous solution	1301:1316	aqueous solution	1301:1316	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	0	72	theme	β-Cyclodextrin-EDTA-Chitosan	16:43	arg1	polymer					45:51	Multifunctional β-Cyclodextrin-EDTA-Chitosan polymer	0:51	Multifunctional β-Cyclodextrin-EDTA-Chitosan polymer	0:51	Multifunctional β-Cyclodextrin-EDTA-Chitosan polymer adsorbent synthesis for simultaneous removal of heavy metals and organic dyes from wastewater.
34742823	7	73	theme	heavy	1888:1892	arg1	Hg2+					1901:1904	Hg2+	1901:1904	Hg2+	1901:1904	In addition, we have also demonstrated the performance of β-CD-EDTA-CS for the of four heavy metals Hg2+, Cd2+, Ni2+, and Cu2+ and three dyes MB, CV, and SO in secondary treated wastewater.
34742823	7	73	theme	heavy	1888:1892	arg1	Ni2+					1913:1916	Ni2+	1913:1916	Ni2+	1913:1916	In addition, we have also demonstrated the performance of β-CD-EDTA-CS for the of four heavy metals Hg2+, Cd2+, Ni2+, and Cu2+ and three dyes MB, CV, and SO in secondary treated wastewater.
34742823	7	73	theme	heavy	1888:1892	arg1	metals					1894:1899	four heavy metals	1883:1899	four heavy metals Hg2+, Cd2+, Ni2+, and Cu2+	1883:1926	In addition, we have also demonstrated the performance of β-CD-EDTA-CS for the of four heavy metals Hg2+, Cd2+, Ni2+, and Cu2+ and three dyes MB, CV, and SO in secondary treated wastewater.
34742823	7	73	theme	heavy	1888:1892	arg1	Cd2+					1907:1910	Cd2+	1907:1910	Cd2+	1907:1910	In addition, we have also demonstrated the performance of β-CD-EDTA-CS for the of four heavy metals Hg2+, Cd2+, Ni2+, and Cu2+ and three dyes MB, CV, and SO in secondary treated wastewater.
34742823	7	73	theme	heavy	1888:1892	arg1	Cu2+					1923:1926	Cu2+	1923:1926	Cu2+	1923:1926	In addition, we have also demonstrated the performance of β-CD-EDTA-CS for the of four heavy metals Hg2+, Cd2+, Ni2+, and Cu2+ and three dyes MB, CV, and SO in secondary treated wastewater.
34742823	6	74	theme	PSO	1617:1619	arg1	behavior					1631:1638	pseudo-second order (PSO) kinetics behavior	1596:1638	pseudo-second order (PSO) kinetics behavior for both metal ions and dyes	1596:1667	Kinetics results followed pseudo-second order (PSO) kinetics behavior for both metal ions and dyes, and higher rate constants values (0.00161-0.00368 g mg-1 min-1) for dyes confirmed the cavitation of organic dyes (physisorption).
34742823	8	75	theme	study	2008:2012	arg1	Findings					1991:1998	Findings	1991:1998	Findings of this study	1991:2012	Findings of this study indicate that β-CD-EDTA-CS simple and essay to synthesize and can be use in wastewater treatment.
34742823	5	76	theme	heterogeneous	1451:1463	arg1	capacity					1476:1483	a heterogeneous adsorption capacity 107.20 ± 5.70	1449:1497	a heterogeneous adsorption capacity 107.20 ± 5.70	1449:1497	The β-CD-EDTA-CS shows monolayer adsorption capacity 346.30 ± 14.0 and 202.90 ± 13.90 mg g-1 for Hg2+ and Cd2+, respectively, and a heterogeneous adsorption capacity 107.20 ± 5.70, 77.40 ± 5.30 and 55.30 ± 3.60 mg g-1 for MB, CV and SO, respectively.
34742823	6	77	theme	order	1610:1614	arg1	behavior					1631:1638	pseudo-second order (PSO) kinetics behavior	1596:1638	pseudo-second order (PSO) kinetics behavior for both metal ions and dyes	1596:1667	Kinetics results followed pseudo-second order (PSO) kinetics behavior for both metal ions and dyes, and higher rate constants values (0.00161-0.00368 g mg-1 min-1) for dyes confirmed the cavitation of organic dyes (physisorption).
34742823	3	78	theme	thermal	993:999	arg1	compositions					962:973	their structural, functional, morphological, elemental compositions	907:973	their structural, functional, morphological, elemental compositions	907:973	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	3	78	theme	thermal	993:999	arg1	properties					1001:1010	thermal properties	993:1010	thermal properties	993:1010	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	8	79	theme	simple	2041:2046	arg1	β-CD-EDTA-CS					2028:2039	β-CD-EDTA-CS simple	2028:2046	β-CD-EDTA-CS simple	2028:2046	Findings of this study indicate that β-CD-EDTA-CS simple and essay to synthesize and can be use in wastewater treatment.
34742823	2	80	theme	inorganic	491:499	arg1	pollutants					513:522	inorganic and organic pollutants	491:522	inorganic and organic pollutants	491:522	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
34742823	6	81	theme	organic	1771:1777	arg1	physisorption					1785:1797	physisorption	1785:1797	physisorption	1785:1797	Kinetics results followed pseudo-second order (PSO) kinetics behavior for both metal ions and dyes, and higher rate constants values (0.00161-0.00368 g mg-1 min-1) for dyes confirmed the cavitation of organic dyes (physisorption).
34742823	6	81	theme	organic	1771:1777	arg1	dyes					1779:1782	organic dyes	1771:1782	organic dyes (physisorption)	1771:1798	Kinetics results followed pseudo-second order (PSO) kinetics behavior for both metal ions and dyes, and higher rate constants values (0.00161-0.00368 g mg-1 min-1) for dyes confirmed the cavitation of organic dyes (physisorption).
34742823	5	82	theme	monolayer	1342:1350	arg1	capacity					1363:1370	monolayer adsorption capacity 346.30 ± 14.0	1342:1384	monolayer adsorption capacity 346.30 ± 14.0	1342:1384	The β-CD-EDTA-CS shows monolayer adsorption capacity 346.30 ± 14.0 and 202.90 ± 13.90 mg g-1 for Hg2+ and Cd2+, respectively, and a heterogeneous adsorption capacity 107.20 ± 5.70, 77.40 ± 5.30 and 55.30 ± 3.60 mg g-1 for MB, CV and SO, respectively.
34742823	4	83	theme	organic	1215:1221	arg1	pollutants					1223:1232	inorganic and organic pollutants	1201:1232	inorganic and organic pollutants	1201:1232	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	4	83	theme	organic	1215:1221	arg1	types					1031:1035	Two types	1027:1035	Two types	1027:1035	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	4	83	theme	organic	1215:1221	arg1	dyes					1113:1116	three organic dyes	1099:1116	three organic dyes	1099:1116	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	3	84	theme	powder	689:694	arg1	Brunauer-Emmett-Teller					824:845	Brunauer-Emmett-Teller	824:845	Brunauer-Emmett-Teller (BET)	824:851	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	3	84	theme	powder	689:694	arg1	microscopy					780:789	field scanning electron microscopy	756:789	field scanning electron microscopy	756:789	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	3	84	theme	powder	689:694	arg1	diffraction					702:712	powder X-ray diffraction	689:712	powder X-ray diffraction	689:712	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	3	84	theme	powder	689:694	arg1	Fourier					715:721	Fourier	715:721	Fourier transform infrared spectroscopy	715:753	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	3	84	theme	powder	689:694	arg1	spectroscopy					810:821	energy dispersive spectroscopy	792:821	energy dispersive spectroscopy	792:821	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	4	85	dep	methylene	1125:1133	arg1	i.e.					1119:1122	i.e.	1119:1122	i.e.	1119:1122	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	4	86	theme	organic	1105:1111	arg1	pollutants					1223:1232	inorganic and organic pollutants	1201:1232	inorganic and organic pollutants	1201:1232	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	4	86	theme	organic	1105:1111	arg1	types					1031:1035	Two types	1027:1035	Two types	1027:1035	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	4	86	theme	organic	1105:1111	arg1	dyes					1113:1116	three organic dyes	1099:1116	three organic dyes	1099:1116	Two types of heavy metals, i.e., mercury (Hg2+) and cadmium (Cd2+), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution.
34742823	3	87	theme	infrared	733:740	arg1	spectroscopy					742:753	infrared spectroscopy	733:753	infrared spectroscopy	733:753	The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively.
34742823	2	88	theme	β-CD	407:410	arg1	reaction					385:392	the reaction	381:392	the reaction of activated β-CD with CS	381:418	Herein, a trifunctional β-cyclodextrin-ethylenediaminetetraacetic acid-chitosan (β-CD-EDTA-CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β-CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc.
32403037	0	0	from	effects	20:26	arg1	micro-					57:62	chitosan/tripolyphosphate micro-	31:62	chitosan/tripolyphosphate micro-	31:62	Biomolecular uptake effects on chitosan/tripolyphosphate micro- and nanoparticle stability.
32403037	0	0	from	effects	20:26	arg1	stability					81:89	nanoparticle stability	68:89	nanoparticle stability	68:89	Biomolecular uptake effects on chitosan/tripolyphosphate micro- and nanoparticle stability.
32403037	1	1	theme	delivery	194:201	arg1	attention					171:179	significant attention	159:179	significant attention	159:179	Colloidal chitosan/tripolyphosphate (TPP) particles have attracted significant attention as potential delivery vehicles for drugs, genes and vaccines.
32403037	1	1	theme	delivery	194:201	arg1	vehicles					203:210	potential delivery vehicles	184:210	potential delivery vehicles for drugs, genes and vaccines	184:240	Colloidal chitosan/tripolyphosphate (TPP) particles have attracted significant attention as potential delivery vehicles for drugs, genes and vaccines.
32403037	7	2	theme	model	1468:1472	arg1	media					1486:1490	model dissolution media	1468:1490	model dissolution media	1468:1490	Conversely, DNA uptake (at least at some compositions) increased the chitosan fractions persisting in a complexed/particulate form in model dissolution media, with the DNA remaining largely complexed to the chitosan at all investigated conditions.
32403037	7	3	theme	complexed/particulate	1438:1458	arg1	form					1460:1463	a complexed/particulate form	1436:1463	a complexed/particulate form in model dissolution media	1436:1490	Conversely, DNA uptake (at least at some compositions) increased the chitosan fractions persisting in a complexed/particulate form in model dissolution media, with the DNA remaining largely complexed to the chitosan at all investigated conditions.
32403037	4	4	theme	ionic	880:884	arg1	strengths					886:894	physiological ionic strengths	866:894	physiological ionic strengths	866:894	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	5	theme	anionic	725:731	arg1	α-lactalbumin					743:755	α-lactalbumin	743:755	α-lactalbumin (α-LA)	743:762	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	5	theme	anionic	725:731	arg1	proteins					733:740	model anionic proteins	719:740	model anionic proteins	719:740	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	5	theme	anionic	725:731	arg1	albumin					781:787	bovine serum albumin	768:787	bovine serum albumin (BSA)	768:793	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	2	6	theme	ionic	360:364	arg1	strength					366:373	ionic strength	360:373	ionic strength	360:373	Yet, there have been several fundamental studies that showed these particles to disintegrate at physiological pH and ionic strength levels.
32403037	8	7	theme	polyanions	1825:1834	arg1	incorporation					1808:1820	the incorporation	1804:1820	the incorporation of polyanions	1804:1834	Collectively, these findings suggest that, while most bioactive payloads do not interact with chitosan strongly enough to stabilize chitosan/TPP particles, these chitosan particles can be stabilized to dissolution through the incorporation of polyanions.
32403037	7	8	theme	DNA	1346:1348	arg1	uptake					1350:1355	DNA uptake	1346:1355	DNA uptake (at least at some compositions)	1346:1387	Conversely, DNA uptake (at least at some compositions) increased the chitosan fractions persisting in a complexed/particulate form in model dissolution media, with the DNA remaining largely complexed to the chitosan at all investigated conditions.
32403037	3	9	theme	published	420:428	arg1	research					462:469	the published drug, gene and vaccine delivery research	416:469	the published drug, gene and vaccine delivery research where chitosan/TPP particle disintegration was not reported	416:529	To reconcile these findings with the published drug, gene and vaccine delivery research where chitosan/TPP particle disintegration was not reported, it has been postulated that the particles could be stabilized by their bioactive payloads.
32403037	4	10	theme	1×	928:929	arg1	NaCl					910:913	150 mM NaCl	903:913	150 mM NaCl (pH 5.5)	903:922	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	10	theme	1×	928:929	arg1	pH					936:937	pH 6.0	936:941	pH 6.0	936:941	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	10	theme	1×	928:929	arg1	PBS					931:933	1× PBS	928:933	1× PBS (pH 6.0)	928:942	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	10	theme	1×	928:929	arg1	media					963:967	the dissolution media	947:967	the dissolution media	947:967	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	7	11	theme	chitosan	1403:1410	arg1	fractions					1412:1420	the chitosan fractions	1399:1420	the chitosan fractions persisting in a complexed/particulate form in model dissolution media	1399:1490	Conversely, DNA uptake (at least at some compositions) increased the chitosan fractions persisting in a complexed/particulate form in model dissolution media, with the DNA remaining largely complexed to the chitosan at all investigated conditions.
32403037	5	12	theme	particle	1065:1072	arg1	stability					1074:1082	particle stability	1065:1082	particle stability	1065:1082	Light scattering and UV-vis spectroscopy revealed that anionic protein uptake had no impact on particle stability, likely due to the relatively weak protein/particle binding at near-physiological ionic strengths, which caused the protein to be rapidly released.
32403037	1	13	theme	Colloidal	92:100	arg1	particles					134:142	Colloidal chitosan/tripolyphosphate (TPP) particles	92:142	Colloidal chitosan/tripolyphosphate (TPP) particles	92:142	Colloidal chitosan/tripolyphosphate (TPP) particles have attracted significant attention as potential delivery vehicles for drugs, genes and vaccines.
32403037	4	14	theme	particles	704:712	arg1	association					676:686	the association	672:686	the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA)	672:820	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	8	15	theme	bioactive	1636:1644	arg1	payloads					1646:1653	most bioactive payloads	1631:1653	most bioactive payloads	1631:1653	Collectively, these findings suggest that, while most bioactive payloads do not interact with chitosan strongly enough to stabilize chitosan/TPP particles, these chitosan particles can be stabilized to dissolution through the incorporation of polyanions.
32403037	4	16	theme	particle	844:851	arg1	stability					853:861	chitosan/TPP particle stability	831:861	chitosan/TPP particle stability	831:861	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	1	17	theme	chitosan/tripolyphosphate	102:126	arg1	particles					134:142	Colloidal chitosan/tripolyphosphate (TPP) particles	92:142	Colloidal chitosan/tripolyphosphate (TPP) particles	92:142	Colloidal chitosan/tripolyphosphate (TPP) particles have attracted significant attention as potential delivery vehicles for drugs, genes and vaccines.
32403037	4	18	theme	physiological	866:878	arg1	strengths					886:894	physiological ionic strengths	866:894	physiological ionic strengths	866:894	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	2	19	theme	physiological	339:351	arg1	pH					353:354	physiological pH	339:354	physiological pH	339:354	Yet, there have been several fundamental studies that showed these particles to disintegrate at physiological pH and ionic strength levels.
32403037	3	20	theme	vaccine	445:451	arg1	delivery					453:460	drug, gene and vaccine delivery	430:460	delivery	453:460	To reconcile these findings with the published drug, gene and vaccine delivery research where chitosan/TPP particle disintegration was not reported, it has been postulated that the particles could be stabilized by their bioactive payloads.
32403037	0	21	theme	Biomolecular	0:11	arg1	uptake					13:18	Biomolecular uptake	0:18	Biomolecular uptake	0:18	Biomolecular uptake effects on chitosan/tripolyphosphate micro- and nanoparticle stability.
32403037	3	22	theme	bioactive	603:611	arg1	payloads					613:620	their bioactive payloads	597:620	their bioactive payloads	597:620	To reconcile these findings with the published drug, gene and vaccine delivery research where chitosan/TPP particle disintegration was not reported, it has been postulated that the particles could be stabilized by their bioactive payloads.
32403037	8	23	theme	chitosan/TPP	1714:1725	arg1	particles					1727:1735	chitosan/TPP particles	1714:1735	chitosan/TPP particles	1714:1735	Collectively, these findings suggest that, while most bioactive payloads do not interact with chitosan strongly enough to stabilize chitosan/TPP particles, these chitosan particles can be stabilized to dissolution through the incorporation of polyanions.
32403037	3	24	theme	delivery	453:460	arg1	research					462:469	the published drug, gene and vaccine delivery research	416:469	the published drug, gene and vaccine delivery research where chitosan/TPP particle disintegration was not reported	416:529	To reconcile these findings with the published drug, gene and vaccine delivery research where chitosan/TPP particle disintegration was not reported, it has been postulated that the particles could be stabilized by their bioactive payloads.
32403037	5	25	contain	had	1048:1050	arg2	impact					1055:1060	no impact	1052:1060	no impact	1052:1060	Light scattering and UV-vis spectroscopy revealed that anionic protein uptake had no impact on particle stability, likely due to the relatively weak protein/particle binding at near-physiological ionic strengths, which caused the protein to be rapidly released.
32403037	5	25	contain	had	1048:1050	arg1	uptake					1041:1046	anionic protein uptake	1025:1046	anionic protein uptake	1025:1046	Light scattering and UV-vis spectroscopy revealed that anionic protein uptake had no impact on particle stability, likely due to the relatively weak protein/particle binding at near-physiological ionic strengths, which caused the protein to be rapidly released.
32403037	5	26	theme	near-physiological	1147:1164	arg1	strengths					1172:1180	near-physiological ionic strengths	1147:1180	near-physiological ionic strengths	1147:1180	Light scattering and UV-vis spectroscopy revealed that anionic protein uptake had no impact on particle stability, likely due to the relatively weak protein/particle binding at near-physiological ionic strengths, which caused the protein to be rapidly released.
32403037	6	27	theme	particle	1314:1321	arg1	formation					1323:1331	particle formation	1314:1331	particle formation	1314:1331	This result occurred regardless of whether the protein was loaded during or after particle formation.
32403037	4	28	theme	serum	775:779	arg1	proteins					733:740	model anionic proteins	719:740	model anionic proteins	719:740	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	28	theme	serum	775:779	arg1	BSA					790:792	BSA	790:792	BSA	790:792	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	28	theme	serum	775:779	arg1	albumin					781:787	bovine serum albumin	768:787	bovine serum albumin (BSA)	768:793	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	1	29	theme	TPP	129:131	arg1	particles					134:142	Colloidal chitosan/tripolyphosphate (TPP) particles	92:142	Colloidal chitosan/tripolyphosphate (TPP) particles	92:142	Colloidal chitosan/tripolyphosphate (TPP) particles have attracted significant attention as potential delivery vehicles for drugs, genes and vaccines.
32403037	5	30	theme	ionic	1166:1170	arg1	strengths					1172:1180	near-physiological ionic strengths	1147:1180	near-physiological ionic strengths	1147:1180	Light scattering and UV-vis spectroscopy revealed that anionic protein uptake had no impact on particle stability, likely due to the relatively weak protein/particle binding at near-physiological ionic strengths, which caused the protein to be rapidly released.
32403037	7	31	theme	remaining	1506:1514	arg1	DNA					1502:1504	the DNA remaining	1498:1514	the DNA remaining largely complexed to the chitosan at all investigated conditions	1498:1579	Conversely, DNA uptake (at least at some compositions) increased the chitosan fractions persisting in a complexed/particulate form in model dissolution media, with the DNA remaining largely complexed to the chitosan at all investigated conditions.
32403037	4	32	theme	bovine	768:773	arg1	proteins					733:740	model anionic proteins	719:740	model anionic proteins	719:740	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	32	theme	bovine	768:773	arg1	BSA					790:792	BSA	790:792	BSA	790:792	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	32	theme	bovine	768:773	arg1	albumin					781:787	bovine serum albumin	768:787	bovine serum albumin (BSA)	768:793	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	5	33	theme	UV-vis	991:996	arg1	spectroscopy					998:1009	UV-vis spectroscopy	991:1009	UV-vis spectroscopy	991:1009	Light scattering and UV-vis spectroscopy revealed that anionic protein uptake had no impact on particle stability, likely due to the relatively weak protein/particle binding at near-physiological ionic strengths, which caused the protein to be rapidly released.
32403037	3	34	theme	gene	436:439	arg1	delivery					453:460	drug, gene and vaccine delivery	430:460	delivery	453:460	To reconcile these findings with the published drug, gene and vaccine delivery research where chitosan/TPP particle disintegration was not reported, it has been postulated that the particles could be stabilized by their bioactive payloads.
32403037	4	35	theme	chitosan/TPP	691:702	arg1	particles					704:712	chitosan/TPP particles	691:712	chitosan/TPP particles	691:712	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	3	36	theme	chitosan/TPP	477:488	arg1	disintegration					499:512	chitosan/TPP particle disintegration	477:512	chitosan/TPP particle disintegration	477:512	To reconcile these findings with the published drug, gene and vaccine delivery research where chitosan/TPP particle disintegration was not reported, it has been postulated that the particles could be stabilized by their bioactive payloads.
32403037	0	37	theme	chitosan/tripolyphosphate	31:55	arg1	micro-					57:62	chitosan/tripolyphosphate micro-	31:62	chitosan/tripolyphosphate micro-	31:62	Biomolecular uptake effects on chitosan/tripolyphosphate micro- and nanoparticle stability.
32403037	4	38	theme	dissolution	951:961	arg1	NaCl					910:913	150 mM NaCl	903:913	150 mM NaCl (pH 5.5)	903:922	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	38	theme	dissolution	951:961	arg1	media					963:967	the dissolution media	947:967	the dissolution media	947:967	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	38	theme	dissolution	951:961	arg1	PBS					931:933	1× PBS	928:933	1× PBS (pH 6.0)	928:942	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	8	39	theme	chitosan	1744:1751	arg1	particles					1753:1761	these chitosan particles	1738:1761	these chitosan particles	1738:1761	Collectively, these findings suggest that, while most bioactive payloads do not interact with chitosan strongly enough to stabilize chitosan/TPP particles, these chitosan particles can be stabilized to dissolution through the incorporation of polyanions.
32403037	3	40	theme	particle	490:497	arg1	disintegration					499:512	chitosan/TPP particle disintegration	477:512	chitosan/TPP particle disintegration	477:512	To reconcile these findings with the published drug, gene and vaccine delivery research where chitosan/TPP particle disintegration was not reported, it has been postulated that the particles could be stabilized by their bioactive payloads.
32403037	3	41	theme	drug	430:433	arg1	delivery					453:460	drug, gene and vaccine delivery	430:460	delivery	453:460	To reconcile these findings with the published drug, gene and vaccine delivery research where chitosan/TPP particle disintegration was not reported, it has been postulated that the particles could be stabilized by their bioactive payloads.
32403037	5	42	theme	weak	1114:1117	arg1	binding					1136:1142	the relatively weak protein/particle binding	1099:1142	the relatively weak protein/particle binding	1099:1142	Light scattering and UV-vis spectroscopy revealed that anionic protein uptake had no impact on particle stability, likely due to the relatively weak protein/particle binding at near-physiological ionic strengths, which caused the protein to be rapidly released.
32403037	4	43	theme	mM	907:908	arg1	NaCl					910:913	150 mM NaCl	903:913	150 mM NaCl (pH 5.5)	903:922	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	43	theme	mM	907:908	arg1	media					963:967	the dissolution media	947:967	the dissolution media	947:967	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	43	theme	mM	907:908	arg1	pH					916:917	pH 5.5	916:921	pH 5.5	916:921	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	43	theme	mM	907:908	arg1	PBS					931:933	1× PBS	928:933	1× PBS (pH 6.0)	928:942	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	44	with	association	676:686	arg1	polynucleotides					800:814	polynucleotides	800:814	polynucleotides (DNA)	800:820	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	44	with	association	676:686	arg1	α-lactalbumin					743:755	α-lactalbumin	743:755	α-lactalbumin (α-LA)	743:762	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	44	with	association	676:686	arg1	proteins					733:740	model anionic proteins	719:740	model anionic proteins	719:740	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	44	with	association	676:686	arg1	albumin					781:787	bovine serum albumin	768:787	bovine serum albumin (BSA)	768:793	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	44	with	association	676:686	arg1	DNA					817:819	DNA	817:819	DNA	817:819	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	45	theme	chitosan/TPP	831:842	arg1	stability					853:861	chitosan/TPP particle stability	831:861	chitosan/TPP particle stability	831:861	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	5	46	theme	protein/particle	1119:1134	arg1	binding					1136:1142	the relatively weak protein/particle binding	1099:1142	the relatively weak protein/particle binding	1099:1142	Light scattering and UV-vis spectroscopy revealed that anionic protein uptake had no impact on particle stability, likely due to the relatively weak protein/particle binding at near-physiological ionic strengths, which caused the protein to be rapidly released.
32403037	4	47	theme	150	903:905	arg1	mM					907:908	mM	907:908	mM	907:908	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	5	48	from	strengths	1172:1180	arg1	binding					1136:1142	the relatively weak protein/particle binding	1099:1142	the relatively weak protein/particle binding	1099:1142	Light scattering and UV-vis spectroscopy revealed that anionic protein uptake had no impact on particle stability, likely due to the relatively weak protein/particle binding at near-physiological ionic strengths, which caused the protein to be rapidly released.
32403037	8	49	theme	most	1631:1634	arg1	payloads					1646:1653	most bioactive payloads	1631:1653	most bioactive payloads	1631:1653	Collectively, these findings suggest that, while most bioactive payloads do not interact with chitosan strongly enough to stabilize chitosan/TPP particles, these chitosan particles can be stabilized to dissolution through the incorporation of polyanions.
32403037	5	50	theme	Light	970:974	arg1	scattering					976:985	Light scattering	970:985	Light scattering	970:985	Light scattering and UV-vis spectroscopy revealed that anionic protein uptake had no impact on particle stability, likely due to the relatively weak protein/particle binding at near-physiological ionic strengths, which caused the protein to be rapidly released.
32403037	7	51	theme	dissolution	1474:1484	arg1	media					1486:1490	model dissolution media	1468:1490	model dissolution media	1468:1490	Conversely, DNA uptake (at least at some compositions) increased the chitosan fractions persisting in a complexed/particulate form in model dissolution media, with the DNA remaining largely complexed to the chitosan at all investigated conditions.
32403037	7	52	from	form	1460:1463	arg1	media					1486:1490	model dissolution media	1468:1490	model dissolution media	1468:1490	Conversely, DNA uptake (at least at some compositions) increased the chitosan fractions persisting in a complexed/particulate form in model dissolution media, with the DNA remaining largely complexed to the chitosan at all investigated conditions.
32403037	1	53	theme	significant	159:169	arg1	attention					171:179	significant attention	159:179	significant attention	159:179	Colloidal chitosan/tripolyphosphate (TPP) particles have attracted significant attention as potential delivery vehicles for drugs, genes and vaccines.
32403037	1	53	theme	significant	159:169	arg1	vehicles					203:210	potential delivery vehicles	184:210	potential delivery vehicles for drugs, genes and vaccines	184:240	Colloidal chitosan/tripolyphosphate (TPP) particles have attracted significant attention as potential delivery vehicles for drugs, genes and vaccines.
32403037	7	54	theme	investigated	1557:1568	arg1	conditions					1570:1579	all investigated conditions	1553:1579	all investigated conditions	1553:1579	Conversely, DNA uptake (at least at some compositions) increased the chitosan fractions persisting in a complexed/particulate form in model dissolution media, with the DNA remaining largely complexed to the chitosan at all investigated conditions.
32403037	5	55	theme	anionic	1025:1031	arg1	uptake					1041:1046	anionic protein uptake	1025:1046	anionic protein uptake	1025:1046	Light scattering and UV-vis spectroscopy revealed that anionic protein uptake had no impact on particle stability, likely due to the relatively weak protein/particle binding at near-physiological ionic strengths, which caused the protein to be rapidly released.
32403037	2	56	dep	pH	353:354	arg1	levels					375:380	levels	375:380	levels	375:380	Yet, there have been several fundamental studies that showed these particles to disintegrate at physiological pH and ionic strength levels.
32403037	5	57	theme	protein	1033:1039	arg1	uptake					1041:1046	anionic protein uptake	1025:1046	anionic protein uptake	1025:1046	Light scattering and UV-vis spectroscopy revealed that anionic protein uptake had no impact on particle stability, likely due to the relatively weak protein/particle binding at near-physiological ionic strengths, which caused the protein to be rapidly released.
32403037	2	58	theme	fundamental	272:282	arg1	studies					284:290	several fundamental studies	264:290	several fundamental studies that showed these particles to disintegrate at physiological pH and ionic strength levels	264:380	Yet, there have been several fundamental studies that showed these particles to disintegrate at physiological pH and ionic strength levels.
32403037	4	59	theme	model	719:723	arg1	α-lactalbumin					743:755	α-lactalbumin	743:755	α-lactalbumin (α-LA)	743:762	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	59	theme	model	719:723	arg1	proteins					733:740	model anionic proteins	719:740	model anionic proteins	719:740	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	4	59	theme	model	719:723	arg1	albumin					781:787	bovine serum albumin	768:787	bovine serum albumin (BSA)	768:793	To test this hypothesis, here we examine whether the association of chitosan/TPP particles with model anionic proteins, α-lactalbumin (α-LA) and bovine serum albumin (BSA), and polynucleotides (DNA) enhances chitosan/TPP particle stability at physiological ionic strengths, using 150 mM NaCl (pH 5.5) and 1× PBS (pH 6.0) as the dissolution media.
32403037	7	60	from	compositions	1375:1386	arg1	uptake					1350:1355	DNA uptake	1346:1355	DNA uptake (at least at some compositions)	1346:1387	Conversely, DNA uptake (at least at some compositions) increased the chitosan fractions persisting in a complexed/particulate form in model dissolution media, with the DNA remaining largely complexed to the chitosan at all investigated conditions.
32403037	0	61	theme	nanoparticle	68:79	arg1	stability					81:89	nanoparticle stability	68:89	nanoparticle stability	68:89	Biomolecular uptake effects on chitosan/tripolyphosphate micro- and nanoparticle stability.
32403037	2	62	theme	several	264:270	arg1	studies					284:290	several fundamental studies	264:290	several fundamental studies that showed these particles to disintegrate at physiological pH and ionic strength levels	264:380	Yet, there have been several fundamental studies that showed these particles to disintegrate at physiological pH and ionic strength levels.
32403037	1	63	theme	potential	184:192	arg1	attention					171:179	significant attention	159:179	significant attention	159:179	Colloidal chitosan/tripolyphosphate (TPP) particles have attracted significant attention as potential delivery vehicles for drugs, genes and vaccines.
32403037	1	63	theme	potential	184:192	arg1	vehicles					203:210	potential delivery vehicles	184:210	potential delivery vehicles for drugs, genes and vaccines	184:240	Colloidal chitosan/tripolyphosphate (TPP) particles have attracted significant attention as potential delivery vehicles for drugs, genes and vaccines.
32693141	8	0	theme	chitosan	971:978	arg1	Crystallinity					944:956	Crystallinity	944:956	Crystallinity of the pupae chitosan	944:978	Crystallinity of the pupae chitosan was 48% compared to 38% for egg shell chitosan.
32693141	10	1	from	viable	1144:1149	arg1	concentrations					1210:1223	all the concentrations	1202:1223	all the concentrations tested	1202:1230	Cells were viable in the presence of pupae and egg shell chitosan in all the concentrations tested.
32693141	10	1	from	viable	1144:1149	arg1	presence					1158:1165	the presence	1154:1165	the presence of pupae and egg shell chitosan	1154:1197	Cells were viable in the presence of pupae and egg shell chitosan in all the concentrations tested.
32693141	6	2	theme	physical	736:743	arg1	structure					745:753	physical structure	736:753	physical structure	736:753	Thermal behavior, physical structure, antimicrobial activity and ability to support the attachment and growth of NIH3T3 cells were studied.
32693141	0	3	theme	egg	68:70	arg1	shells					72:77	egg shells	68:77	egg shells	68:77	Properties of chitin and chitosan extracted from silkworm pupae and egg shells.
32693141	10	4	from	presence	1158:1165	arg1	viable					1144:1149	viable	1144:1149	viable	1144:1149	Cells were viable in the presence of pupae and egg shell chitosan in all the concentrations tested.
32693141	4	5	from	chitin	509:514	arg1	comparison					567:576	comparison	567:576	comparison to commercially available chitosan	567:611	In this paper, we report the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan.
32693141	6	6	theme	cells	838:842	arg1	attachment					806:815	attachment	806:815	attachment	806:815	Thermal behavior, physical structure, antimicrobial activity and ability to support the attachment and growth of NIH3T3 cells were studied.
32693141	6	6	theme	cells	838:842	arg1	growth					821:826	growth	821:826	growth	821:826	Thermal behavior, physical structure, antimicrobial activity and ability to support the attachment and growth of NIH3T3 cells were studied.
32693141	1	7	theme	distinct	140:147	arg1	properties					149:158	distinct properties	140:158	distinct properties with excellent antimicrobial properties and cytocompatiblity	140:219	Chitin and chitosan from silkworm pupae and egg shells show distinct properties with excellent antimicrobial properties and cytocompatiblity.
32693141	9	8	theme	higher	1066:1071	arg1	activity					1102:1109	considerably higher antibacterial and antifungal activity	1053:1109	considerably higher antibacterial and antifungal activity	1053:1109	Silkworm chitosan showed considerably higher antibacterial and antifungal activity compared to standard.
32693141	3	9	theme	excellent	359:367	arg1	properties					383:392	excellent antimicrobial properties	359:392	excellent antimicrobial properties	359:392	Chitosan has excellent antimicrobial properties and is widely used for food, medical and biotechnological applications.
32693141	4	10	theme	chitin	509:514	arg1	properties					495:504	the properties	491:504	the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan	491:611	In this paper, we report the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan.
32693141	7	11	contain	had	905:907	arg2	composition					931:941	composition	931:941	composition	931:941	Chitin and chitosan from both pupae and shells had similar structure and composition.
32693141	7	11	contain	had	905:907	arg1	chitosan					869:876	chitosan	869:876	chitosan	869:876	Chitin and chitosan from both pupae and shells had similar structure and composition.
32693141	7	11	contain	had	905:907	arg2	structure					917:925	similar structure	909:925	similar structure	909:925	Chitin and chitosan from both pupae and shells had similar structure and composition.
32693141	7	11	contain	had	905:907	arg1	Chitin					858:863	Chitin	858:863	Chitin	858:863	Chitin and chitosan from both pupae and shells had similar structure and composition.
32693141	6	12	theme	NIH3T3	831:836	arg1	cells					838:842	NIH3T3 cells	831:842	NIH3T3 cells	831:842	Thermal behavior, physical structure, antimicrobial activity and ability to support the attachment and growth of NIH3T3 cells were studied.
32693141	7	13	from	pupae	888:892	arg1	chitosan					869:876	chitosan	869:876	chitosan	869:876	Chitin and chitosan from both pupae and shells had similar structure and composition.
32693141	7	13	from	pupae	888:892	arg1	Chitin					858:863	Chitin	858:863	Chitin	858:863	Chitin and chitosan from both pupae and shells had similar structure and composition.
32693141	9	14	theme	antibacterial	1073:1085	arg1	activity					1102:1109	considerably higher antibacterial and antifungal activity	1053:1109	considerably higher antibacterial and antifungal activity	1053:1109	Silkworm chitosan showed considerably higher antibacterial and antifungal activity compared to standard.
32693141	4	15	theme	available	594:602	arg1	chitosan					604:611	commercially available chitosan	581:611	commercially available chitosan	581:611	In this paper, we report the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan.
32693141	3	16	theme	antimicrobial	369:381	arg1	properties					383:392	excellent antimicrobial properties	359:392	excellent antimicrobial properties	359:392	Chitosan has excellent antimicrobial properties and is widely used for food, medical and biotechnological applications.
32693141	4	17	from	properties	495:504	arg1	comparison					567:576	comparison	567:576	comparison to commercially available chitosan	567:611	In this paper, we report the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan.
32693141	4	17	from	properties	495:504	arg1	pupae					543:547	silkworm pupae	534:547	silkworm pupae	534:547	In this paper, we report the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan.
32693141	4	17	from	properties	495:504	arg1	shells					557:562	egg shells	553:562	egg shells	553:562	In this paper, we report the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan.
32693141	5	18	theme	Defatted	614:621	arg1	pupae					641:645	pupae	641:645	pupae	641:645	Defatted and deproteinated pupae and shells were demineralized and later deacetylated to form chitosan.
32693141	5	19	theme	deproteinated	627:639	arg1	pupae					641:645	pupae	641:645	pupae	641:645	Defatted and deproteinated pupae and shells were demineralized and later deacetylated to form chitosan.
32693141	1	20	from	shells	128:133	arg1	Chitin					80:85	Chitin	80:85	Chitin	80:85	Chitin and chitosan from silkworm pupae and egg shells show distinct properties with excellent antimicrobial properties and cytocompatiblity.
32693141	1	20	from	shells	128:133	arg1	chitosan					91:98	chitosan	91:98	chitosan	91:98	Chitin and chitosan from silkworm pupae and egg shells show distinct properties with excellent antimicrobial properties and cytocompatiblity.
32693141	7	21	from	shells	898:903	arg1	chitosan					869:876	chitosan	869:876	chitosan	869:876	Chitin and chitosan from both pupae and shells had similar structure and composition.
32693141	7	21	from	shells	898:903	arg1	Chitin					858:863	Chitin	858:863	Chitin	858:863	Chitin and chitosan from both pupae and shells had similar structure and composition.
32693141	1	22	theme	excellent	165:173	arg1	properties					189:198	excellent antimicrobial properties	165:198	excellent antimicrobial properties	165:198	Chitin and chitosan from silkworm pupae and egg shells show distinct properties with excellent antimicrobial properties and cytocompatiblity.
32693141	3	23	theme	biotechnological	435:450	arg1	applications					452:463	food, medical and biotechnological applications	417:463	food, medical and biotechnological applications	417:463	Chitosan has excellent antimicrobial properties and is widely used for food, medical and biotechnological applications.
32693141	4	24	from	comparison	567:576	arg1	properties					495:504	the properties	491:504	the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan	491:611	In this paper, we report the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan.
32693141	4	25	from	pupae	543:547	arg1	chitosan					520:527	chitosan	520:527	chitosan	520:527	In this paper, we report the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan.
32693141	4	25	from	pupae	543:547	arg1	chitin					509:514	chitin	509:514	chitin	509:514	In this paper, we report the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan.
32693141	4	25	from	pupae	543:547	arg1	properties					495:504	the properties	491:504	the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan	491:611	In this paper, we report the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan.
32693141	1	26	from	pupae	114:118	arg1	Chitin					80:85	Chitin	80:85	Chitin	80:85	Chitin and chitosan from silkworm pupae and egg shells show distinct properties with excellent antimicrobial properties and cytocompatiblity.
32693141	1	26	from	pupae	114:118	arg1	chitosan					91:98	chitosan	91:98	chitosan	91:98	Chitin and chitosan from silkworm pupae and egg shells show distinct properties with excellent antimicrobial properties and cytocompatiblity.
32693141	11	27	theme	sustainable	1336:1346	arg1	source					1348:1353	a renewable and sustainable source	1320:1353	a renewable and sustainable source for chitosan with properties suitable for food and medical applications	1320:1425	Based on these observations, it can be inferred that silkworm pupae and shells provide a renewable and sustainable source for chitosan with properties suitable for food and medical applications.
32693141	6	28	theme	Thermal	718:724	arg1	behavior					726:733	Thermal behavior	718:733	Thermal behavior	718:733	Thermal behavior, physical structure, antimicrobial activity and ability to support the attachment and growth of NIH3T3 cells were studied.
32693141	11	29	theme	suitable	1384:1391	arg1	properties					1373:1382	properties	1373:1382	properties suitable for food and medical applications	1373:1425	Based on these observations, it can be inferred that silkworm pupae and shells provide a renewable and sustainable source for chitosan with properties suitable for food and medical applications.
32693141	6	30	dep	attachment	806:815	arg1	the					802:804	the	802:804	the	802:804	Thermal behavior, physical structure, antimicrobial activity and ability to support the attachment and growth of NIH3T3 cells were studied.
32693141	3	31	theme	medical	423:429	arg1	applications					452:463	food, medical and biotechnological applications	417:463	food, medical and biotechnological applications	417:463	Chitosan has excellent antimicrobial properties and is widely used for food, medical and biotechnological applications.
32693141	4	32	from	shells	557:562	arg1	chitosan					520:527	chitosan	520:527	chitosan	520:527	In this paper, we report the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan.
32693141	4	32	from	shells	557:562	arg1	chitin					509:514	chitin	509:514	chitin	509:514	In this paper, we report the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan.
32693141	4	32	from	shells	557:562	arg1	properties					495:504	the properties	491:504	the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan	491:611	In this paper, we report the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan.
32693141	4	33	theme	egg	553:555	arg1	shells					557:562	egg shells	553:562	egg shells	553:562	In this paper, we report the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan.
32693141	8	34	theme	shell	1012:1016	arg1	chitosan					1018:1025	egg shell chitosan	1008:1025	egg shell chitosan	1008:1025	Crystallinity of the pupae chitosan was 48% compared to 38% for egg shell chitosan.
32693141	11	35	with	source	1348:1353	arg1	properties					1373:1382	properties	1373:1382	properties suitable for food and medical applications	1373:1425	Based on these observations, it can be inferred that silkworm pupae and shells provide a renewable and sustainable source for chitosan with properties suitable for food and medical applications.
32693141	1	36	theme	antimicrobial	175:187	arg1	properties					189:198	excellent antimicrobial properties	165:198	excellent antimicrobial properties	165:198	Chitin and chitosan from silkworm pupae and egg shells show distinct properties with excellent antimicrobial properties and cytocompatiblity.
32693141	11	37	theme	renewable	1322:1330	arg1	source					1348:1353	a renewable and sustainable source	1320:1353	a renewable and sustainable source for chitosan with properties suitable for food and medical applications	1320:1425	Based on these observations, it can be inferred that silkworm pupae and shells provide a renewable and sustainable source for chitosan with properties suitable for food and medical applications.
32693141	10	38	theme	pupae	1170:1174	arg1	presence					1158:1165	the presence	1154:1165	the presence of pupae and egg shell chitosan	1154:1197	Cells were viable in the presence of pupae and egg shell chitosan in all the concentrations tested.
32693141	8	39	theme	egg	1008:1010	arg1	chitosan					1018:1025	egg shell chitosan	1008:1025	egg shell chitosan	1008:1025	Crystallinity of the pupae chitosan was 48% compared to 38% for egg shell chitosan.
32693141	2	40	theme	valuable	301:308	arg1	carbohydrates					310:322	valuable carbohydrates	301:322	valuable carbohydrates	301:322	Spent silkworm pupae and hatched egg shells are discarded as waste but contain valuable carbohydrates, proteins and lipids.
32693141	0	41	theme	chitin	14:19	arg1	Properties					0:9	Properties	0:9	Properties of chitin and chitosan	0:32	Properties of chitin and chitosan extracted from silkworm pupae and egg shells.
32693141	3	42	theme	food	417:420	arg1	applications					452:463	food, medical and biotechnological applications	417:463	food, medical and biotechnological applications	417:463	Chitosan has excellent antimicrobial properties and is widely used for food, medical and biotechnological applications.
32693141	9	43	theme	antifungal	1091:1100	arg1	activity					1102:1109	considerably higher antibacterial and antifungal activity	1053:1109	considerably higher antibacterial and antifungal activity	1053:1109	Silkworm chitosan showed considerably higher antibacterial and antifungal activity compared to standard.
32693141	11	44	theme	silkworm	1286:1293	arg1	pupae					1295:1299	silkworm pupae	1286:1299	silkworm pupae	1286:1299	Based on these observations, it can be inferred that silkworm pupae and shells provide a renewable and sustainable source for chitosan with properties suitable for food and medical applications.
32693141	0	45	theme	chitosan	25:32	arg1	Properties					0:9	Properties	0:9	Properties of chitin and chitosan	0:32	Properties of chitin and chitosan extracted from silkworm pupae and egg shells.
32693141	2	46	contain	contain	293:299	arg1	pupae					237:241	Spent silkworm pupae	222:241	Spent silkworm pupae	222:241	Spent silkworm pupae and hatched egg shells are discarded as waste but contain valuable carbohydrates, proteins and lipids.
32693141	2	46	contain	contain	293:299	arg2	proteins					325:332	proteins	325:332	proteins	325:332	Spent silkworm pupae and hatched egg shells are discarded as waste but contain valuable carbohydrates, proteins and lipids.
32693141	2	46	contain	contain	293:299	arg2	lipids					338:343	lipids	338:343	lipids	338:343	Spent silkworm pupae and hatched egg shells are discarded as waste but contain valuable carbohydrates, proteins and lipids.
32693141	2	46	contain	contain	293:299	arg1	waste					283:287	waste	283:287	waste	283:287	Spent silkworm pupae and hatched egg shells are discarded as waste but contain valuable carbohydrates, proteins and lipids.
32693141	2	46	contain	contain	293:299	arg2	carbohydrates					310:322	valuable carbohydrates	301:322	valuable carbohydrates	301:322	Spent silkworm pupae and hatched egg shells are discarded as waste but contain valuable carbohydrates, proteins and lipids.
32693141	2	46	contain	contain	293:299	arg1	shells					259:264	hatched egg shells	247:264	hatched egg shells	247:264	Spent silkworm pupae and hatched egg shells are discarded as waste but contain valuable carbohydrates, proteins and lipids.
32693141	3	47	contain	has	355:357	arg2	properties					383:392	excellent antimicrobial properties	359:392	excellent antimicrobial properties	359:392	Chitosan has excellent antimicrobial properties and is widely used for food, medical and biotechnological applications.
32693141	3	47	contain	has	355:357	arg1	Chitosan					346:353	Chitosan	346:353	Chitosan	346:353	Chitosan has excellent antimicrobial properties and is widely used for food, medical and biotechnological applications.
32693141	11	48	theme	medical	1406:1412	arg1	applications					1414:1425	food and medical applications	1397:1425	food and medical applications	1397:1425	Based on these observations, it can be inferred that silkworm pupae and shells provide a renewable and sustainable source for chitosan with properties suitable for food and medical applications.
32693141	0	49	theme	silkworm	49:56	arg1	pupae					58:62	silkworm pupae	49:62	silkworm pupae	49:62	Properties of chitin and chitosan extracted from silkworm pupae and egg shells.
32693141	4	50	theme	chitosan	520:527	arg1	properties					495:504	the properties	491:504	the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan	491:611	In this paper, we report the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan.
32693141	2	51	theme	silkworm	228:235	arg1	pupae					237:241	Spent silkworm pupae	222:241	Spent silkworm pupae	222:241	Spent silkworm pupae and hatched egg shells are discarded as waste but contain valuable carbohydrates, proteins and lipids.
32693141	2	51	theme	silkworm	228:235	arg1	waste					283:287	waste	283:287	waste	283:287	Spent silkworm pupae and hatched egg shells are discarded as waste but contain valuable carbohydrates, proteins and lipids.
32693141	2	51	theme	silkworm	228:235	arg1	shells					259:264	hatched egg shells	247:264	hatched egg shells	247:264	Spent silkworm pupae and hatched egg shells are discarded as waste but contain valuable carbohydrates, proteins and lipids.
32693141	4	52	from	chitosan	520:527	arg1	comparison					567:576	comparison	567:576	comparison to commercially available chitosan	567:611	In this paper, we report the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan.
32693141	3	53	used	used	408:411	arg2	Chitosan					346:353	Chitosan	346:353	Chitosan	346:353	Chitosan has excellent antimicrobial properties and is widely used for food, medical and biotechnological applications.
32693141	2	54	theme	Spent	222:226	arg1	pupae					237:241	Spent silkworm pupae	222:241	Spent silkworm pupae	222:241	Spent silkworm pupae and hatched egg shells are discarded as waste but contain valuable carbohydrates, proteins and lipids.
32693141	2	54	theme	Spent	222:226	arg1	waste					283:287	waste	283:287	waste	283:287	Spent silkworm pupae and hatched egg shells are discarded as waste but contain valuable carbohydrates, proteins and lipids.
32693141	2	54	theme	Spent	222:226	arg1	shells					259:264	hatched egg shells	247:264	hatched egg shells	247:264	Spent silkworm pupae and hatched egg shells are discarded as waste but contain valuable carbohydrates, proteins and lipids.
32693141	1	55	theme	silkworm	105:112	arg1	pupae					114:118	silkworm pupae	105:118	silkworm pupae	105:118	Chitin and chitosan from silkworm pupae and egg shells show distinct properties with excellent antimicrobial properties and cytocompatiblity.
32693141	4	56	theme	silkworm	534:541	arg1	pupae					543:547	silkworm pupae	534:547	silkworm pupae	534:547	In this paper, we report the properties of chitin and chitosan from silkworm pupae and egg shells in comparison to commercially available chitosan.
32693141	1	57	with	properties	149:158	arg1	cytocompatiblity					204:219	cytocompatiblity	204:219	cytocompatiblity	204:219	Chitin and chitosan from silkworm pupae and egg shells show distinct properties with excellent antimicrobial properties and cytocompatiblity.
32693141	1	57	with	properties	149:158	arg1	properties					189:198	excellent antimicrobial properties	165:198	excellent antimicrobial properties	165:198	Chitin and chitosan from silkworm pupae and egg shells show distinct properties with excellent antimicrobial properties and cytocompatiblity.
32693141	10	58	from	concentrations	1210:1223	arg1	viable					1144:1149	viable	1144:1149	viable	1144:1149	Cells were viable in the presence of pupae and egg shell chitosan in all the concentrations tested.
32693141	9	59	theme	Silkworm	1028:1035	arg1	chitosan					1037:1044	Silkworm chitosan	1028:1044	Silkworm chitosan	1028:1044	Silkworm chitosan showed considerably higher antibacterial and antifungal activity compared to standard.
32693141	6	60	theme	antimicrobial	756:768	arg1	activity					770:777	antimicrobial activity	756:777	antimicrobial activity	756:777	Thermal behavior, physical structure, antimicrobial activity and ability to support the attachment and growth of NIH3T3 cells were studied.
32693141	7	61	theme	similar	909:915	arg1	structure					917:925	similar structure	909:925	similar structure	909:925	Chitin and chitosan from both pupae and shells had similar structure and composition.
32693141	10	62	theme	chitosan	1190:1197	arg1	presence					1158:1165	the presence	1154:1165	the presence of pupae and egg shell chitosan	1154:1197	Cells were viable in the presence of pupae and egg shell chitosan in all the concentrations tested.
32693141	2	63	theme	egg	255:257	arg1	waste					283:287	waste	283:287	waste	283:287	Spent silkworm pupae and hatched egg shells are discarded as waste but contain valuable carbohydrates, proteins and lipids.
32693141	2	63	theme	egg	255:257	arg1	pupae					237:241	Spent silkworm pupae	222:241	Spent silkworm pupae	222:241	Spent silkworm pupae and hatched egg shells are discarded as waste but contain valuable carbohydrates, proteins and lipids.
32693141	2	63	theme	egg	255:257	arg1	shells					259:264	hatched egg shells	247:264	hatched egg shells	247:264	Spent silkworm pupae and hatched egg shells are discarded as waste but contain valuable carbohydrates, proteins and lipids.
32693141	8	64	theme	pupae	965:969	arg1	chitosan					971:978	the pupae chitosan	961:978	the pupae chitosan	961:978	Crystallinity of the pupae chitosan was 48% compared to 38% for egg shell chitosan.
32693141	1	65	theme	egg	124:126	arg1	shells					128:133	egg shells	124:133	egg shells	124:133	Chitin and chitosan from silkworm pupae and egg shells show distinct properties with excellent antimicrobial properties and cytocompatiblity.
32693141	10	66	theme	shell	1184:1188	arg1	chitosan					1190:1197	egg shell chitosan	1180:1197	egg shell chitosan	1180:1197	Cells were viable in the presence of pupae and egg shell chitosan in all the concentrations tested.
32693141	11	67	theme	food	1397:1400	arg1	applications					1414:1425	food and medical applications	1397:1425	food and medical applications	1397:1425	Based on these observations, it can be inferred that silkworm pupae and shells provide a renewable and sustainable source for chitosan with properties suitable for food and medical applications.
32693141	2	68	theme	hatched	247:253	arg1	waste					283:287	waste	283:287	waste	283:287	Spent silkworm pupae and hatched egg shells are discarded as waste but contain valuable carbohydrates, proteins and lipids.
32693141	2	68	theme	hatched	247:253	arg1	pupae					237:241	Spent silkworm pupae	222:241	Spent silkworm pupae	222:241	Spent silkworm pupae and hatched egg shells are discarded as waste but contain valuable carbohydrates, proteins and lipids.
32693141	2	68	theme	hatched	247:253	arg1	shells					259:264	hatched egg shells	247:264	hatched egg shells	247:264	Spent silkworm pupae and hatched egg shells are discarded as waste but contain valuable carbohydrates, proteins and lipids.
32693141	10	69	theme	egg	1180:1182	arg1	chitosan					1190:1197	egg shell chitosan	1180:1197	egg shell chitosan	1180:1197	Cells were viable in the presence of pupae and egg shell chitosan in all the concentrations tested.
32172831	5	0	dep	rough	642:646	arg1	to					648:649	to	648:649	to	648:649	Morphologically, surface of the fibers varied from rough to smooth depending on the extent of treatment.
32172831	5	0	dep	rough	642:646	arg1	smooth					651:656	smooth	651:656	smooth	651:656	Morphologically, surface of the fibers varied from rough to smooth depending on the extent of treatment.
32172831	4	1	theme	hemicellulose	465:477	arg1	amount					455:460	significant amount	443:460	significant amount of hemicellulose and lignin	443:488	Results revealed that significant amount of hemicellulose and lignin was lost after the alkali treatment and bleaching leading to a highly cellulosic fiber (up to 71 %).
32172831	4	1	theme	hemicellulose	465:477	arg1	lignin					483:488	lignin	483:488	lignin	483:488	Results revealed that significant amount of hemicellulose and lignin was lost after the alkali treatment and bleaching leading to a highly cellulosic fiber (up to 71 %).
32172831	4	1	theme	hemicellulose	465:477	arg1	hemicellulose					465:477	hemicellulose	465:477	hemicellulose	465:477	Results revealed that significant amount of hemicellulose and lignin was lost after the alkali treatment and bleaching leading to a highly cellulosic fiber (up to 71 %).
32172831	7	2	theme	negative	857:864	arg1	bacteria					884:891	common gram negative and gram positive bacteria	845:891	common gram negative and gram positive bacteria	845:891	Sausage fibers also possessed anti-microbial activity against common gram negative and gram positive bacteria.
32172831	4	3	theme	lignin	483:488	arg1	amount					455:460	significant amount	443:460	significant amount of hemicellulose and lignin	443:488	Results revealed that significant amount of hemicellulose and lignin was lost after the alkali treatment and bleaching leading to a highly cellulosic fiber (up to 71 %).
32172831	4	3	theme	lignin	483:488	arg1	lignin					483:488	lignin	483:488	lignin	483:488	Results revealed that significant amount of hemicellulose and lignin was lost after the alkali treatment and bleaching leading to a highly cellulosic fiber (up to 71 %).
32172831	4	3	theme	lignin	483:488	arg1	hemicellulose					465:477	hemicellulose	465:477	hemicellulose	465:477	Results revealed that significant amount of hemicellulose and lignin was lost after the alkali treatment and bleaching leading to a highly cellulosic fiber (up to 71 %).
32172831	8	4	theme	unconventional	1002:1015	arg1	sources					1017:1023	many unconventional sources	997:1023	many unconventional sources	997:1023	Overall, sausage fibers have properties similar to that of cotton and better than fibers obtained from many unconventional sources.
32172831	7	5	theme	common	845:850	arg1	bacteria					884:891	common gram negative and gram positive bacteria	845:891	common gram negative and gram positive bacteria	845:891	Sausage fibers also possessed anti-microbial activity against common gram negative and gram positive bacteria.
32172831	4	6	theme	cellulosic	560:569	arg1	%					587:587	up to 71 %	578:587	up to 71 %	578:587	Results revealed that significant amount of hemicellulose and lignin was lost after the alkali treatment and bleaching leading to a highly cellulosic fiber (up to 71 %).
32172831	4	6	theme	cellulosic	560:569	arg1	fiber					571:575	a highly cellulosic fiber	551:575	a highly cellulosic fiber (up to 71 %)	551:588	Results revealed that significant amount of hemicellulose and lignin was lost after the alkali treatment and bleaching leading to a highly cellulosic fiber (up to 71 %).
32172831	4	7	theme	significant	443:453	arg1	amount					455:460	significant amount	443:460	significant amount of hemicellulose and lignin	443:488	Results revealed that significant amount of hemicellulose and lignin was lost after the alkali treatment and bleaching leading to a highly cellulosic fiber (up to 71 %).
32172831	4	7	theme	significant	443:453	arg1	lignin					483:488	lignin	483:488	lignin	483:488	Results revealed that significant amount of hemicellulose and lignin was lost after the alkali treatment and bleaching leading to a highly cellulosic fiber (up to 71 %).
32172831	4	7	theme	significant	443:453	arg1	hemicellulose					465:477	hemicellulose	465:477	hemicellulose	465:477	Results revealed that significant amount of hemicellulose and lignin was lost after the alkali treatment and bleaching leading to a highly cellulosic fiber (up to 71 %).
32172831	8	8	theme	many	997:1000	arg1	sources					1017:1023	many unconventional sources	997:1023	many unconventional sources	997:1023	Overall, sausage fibers have properties similar to that of cotton and better than fibers obtained from many unconventional sources.
32172831	7	9	theme	anti-microbial	813:826	arg1	activity					828:835	anti-microbial activity	813:835	anti-microbial activity against common gram negative and gram positive bacteria	813:891	Sausage fibers also possessed anti-microbial activity against common gram negative and gram positive bacteria.
32172831	3	10	theme	cellulose	304:312	arg1	fibers					314:319	natural cellulose fibers	296:319	natural cellulose fibers	296:319	In this research, natural cellulose fibers were extracted from the fruit using NaOH and later bleached and characterized for their properties.
32172831	8	11	theme	better	964:969	arg1	properties					923:932	properties	923:932	properties similar to that of cotton and better than fibers obtained from many unconventional sources	923:1023	Overall, sausage fibers have properties similar to that of cotton and better than fibers obtained from many unconventional sources.
32172831	1	12	theme	Kigelia	83:89	arg1	africana					91:98	Kigelia africana	83:98	Kigelia africana	83:98	Kigelia africana also known as sausage plant, yields highly fibrous fruit with a hard shell.
32172831	9	13	theme	anti-bacterial	1059:1072	arg1	properties					1074:1083	anti-bacterial properties	1059:1083	anti-bacterial properties	1059:1083	With improved hydrophobicity and anti-bacterial properties, sausage fibers could be potentially applied in functional polymer composites.
32172831	1	14	theme	hard	164:167	arg1	shell					169:173	a hard shell	162:173	a hard shell	162:173	Kigelia africana also known as sausage plant, yields highly fibrous fruit with a hard shell.
32172831	4	15	dep	treatment	516:524	arg1	the					505:507	the	505:507	the	505:507	Results revealed that significant amount of hemicellulose and lignin was lost after the alkali treatment and bleaching leading to a highly cellulosic fiber (up to 71 %).
32172831	7	16	theme	Sausage	783:789	arg1	fibers					791:796	Sausage fibers	783:796	Sausage fibers	783:796	Sausage fibers also possessed anti-microbial activity against common gram negative and gram positive bacteria.
32172831	9	17	theme	sausage	1086:1092	arg1	fibers					1094:1099	sausage fibers	1086:1099	sausage fibers	1086:1099	With improved hydrophobicity and anti-bacterial properties, sausage fibers could be potentially applied in functional polymer composites.
32172831	2	18	theme	sausage	263:269	arg1	trees					271:275	sausage trees	263:275	sausage trees	263:275	Many medicinal uses are reported for the extracts from the fruits, seeds and leaves of sausage trees.
32172831	0	19	theme	cellulose	43:51	arg1	fibers					53:58	natural cellulose fibers	35:58	natural cellulose fibers	35:58	Extraction and characterisation of natural cellulose fibers from Kigelia africana.
32172831	8	20	contain	have	918:921	arg2	properties					923:932	properties	923:932	properties similar to that of cotton and better than fibers obtained from many unconventional sources	923:1023	Overall, sausage fibers have properties similar to that of cotton and better than fibers obtained from many unconventional sources.
32172831	8	20	contain	have	918:921	arg1	fibers					911:916	sausage fibers	903:916	sausage fibers	903:916	Overall, sausage fibers have properties similar to that of cotton and better than fibers obtained from many unconventional sources.
32172831	2	21	from	fruits	235:240	arg1	extracts					217:224	the extracts	213:224	the extracts from the fruits, seeds and leaves of sausage trees	213:275	Many medicinal uses are reported for the extracts from the fruits, seeds and leaves of sausage trees.
32172831	0	22	theme	natural	35:41	arg1	fibers					53:58	natural cellulose fibers	35:58	natural cellulose fibers	35:58	Extraction and characterisation of natural cellulose fibers from Kigelia africana.
32172831	0	23	from	africana	73:80	arg1	characterisation					15:30	characterisation	15:30	characterisation	15:30	Extraction and characterisation of natural cellulose fibers from Kigelia africana.
32172831	0	23	from	africana	73:80	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and characterisation of natural cellulose fibers from Kigelia africana.
32172831	6	24	theme	thermal	700:706	arg1	stability					708:716	The thermal stability	696:716	The thermal stability	696:716	The thermal stability, crystallinity and hydrophobicity increased after the treatment.
32172831	7	25	contain	possessed	803:811	arg2	activity					828:835	anti-microbial activity	813:835	anti-microbial activity against common gram negative and gram positive bacteria	813:891	Sausage fibers also possessed anti-microbial activity against common gram negative and gram positive bacteria.
32172831	7	25	contain	possessed	803:811	arg1	fibers					791:796	Sausage fibers	783:796	Sausage fibers	783:796	Sausage fibers also possessed anti-microbial activity against common gram negative and gram positive bacteria.
32172831	0	26	theme	Kigelia	65:71	arg1	africana					73:80	Kigelia africana	65:80	Kigelia africana	65:80	Extraction and characterisation of natural cellulose fibers from Kigelia africana.
32172831	1	27	theme	sausage	114:120	arg1	plant					122:126	sausage plant	114:126	sausage plant	114:126	Kigelia africana also known as sausage plant, yields highly fibrous fruit with a hard shell.
32172831	0	28	theme	fibers	53:58	arg1	characterisation					15:30	characterisation	15:30	characterisation	15:30	Extraction and characterisation of natural cellulose fibers from Kigelia africana.
32172831	0	28	theme	fibers	53:58	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and characterisation of natural cellulose fibers from Kigelia africana.
32172831	4	29	theme	alkali	509:514	arg1	treatment					516:524	alkali treatment	509:524	alkali treatment	509:524	Results revealed that significant amount of hemicellulose and lignin was lost after the alkali treatment and bleaching leading to a highly cellulosic fiber (up to 71 %).
32172831	5	30	theme	fibers	623:628	arg1	surface					608:614	surface	608:614	surface of the fibers	608:628	Morphologically, surface of the fibers varied from rough to smooth depending on the extent of treatment.
32172831	4	31	dep	71	584:585	arg1	to					581:582	to	581:582	to	581:582	Results revealed that significant amount of hemicellulose and lignin was lost after the alkali treatment and bleaching leading to a highly cellulosic fiber (up to 71 %).
32172831	8	32	theme	sausage	903:909	arg1	fibers					911:916	sausage fibers	903:916	sausage fibers	903:916	Overall, sausage fibers have properties similar to that of cotton and better than fibers obtained from many unconventional sources.
32172831	7	33	theme	positive	875:882	arg1	bacteria					884:891	common gram negative and gram positive bacteria	845:891	common gram negative and gram positive bacteria	845:891	Sausage fibers also possessed anti-microbial activity against common gram negative and gram positive bacteria.
32172831	2	34	dep	medicinal	181:189	arg1	uses					191:194	uses	191:194	uses	191:194	Many medicinal uses are reported for the extracts from the fruits, seeds and leaves of sausage trees.
32172831	9	35	theme	functional	1133:1142	arg1	composites					1152:1161	functional polymer composites	1133:1161	functional polymer composites	1133:1161	With improved hydrophobicity and anti-bacterial properties, sausage fibers could be potentially applied in functional polymer composites.
32172831	5	36	theme	treatment	685:693	arg1	extent					675:680	the extent	671:680	the extent of treatment	671:693	Morphologically, surface of the fibers varied from rough to smooth depending on the extent of treatment.
32172831	9	37	theme	improved	1031:1038	arg1	hydrophobicity					1040:1053	improved hydrophobicity	1031:1053	improved hydrophobicity	1031:1053	With improved hydrophobicity and anti-bacterial properties, sausage fibers could be potentially applied in functional polymer composites.
32172831	9	38	theme	polymer	1144:1150	arg1	composites					1152:1161	functional polymer composites	1133:1161	functional polymer composites	1133:1161	With improved hydrophobicity and anti-bacterial properties, sausage fibers could be potentially applied in functional polymer composites.
32172831	3	39	theme	natural	296:302	arg1	fibers					314:319	natural cellulose fibers	296:319	natural cellulose fibers	296:319	In this research, natural cellulose fibers were extracted from the fruit using NaOH and later bleached and characterized for their properties.
32172831	8	40	theme	similar	934:940	arg1	properties					923:932	properties	923:932	properties similar to that of cotton and better than fibers obtained from many unconventional sources	923:1023	Overall, sausage fibers have properties similar to that of cotton and better than fibers obtained from many unconventional sources.
32172831	1	41	theme	fibrous	143:149	arg1	fruit					151:155	highly fibrous fruit	136:155	highly fibrous fruit	136:155	Kigelia africana also known as sausage plant, yields highly fibrous fruit with a hard shell.
33781814	5	0	theme	synergistic	772:782	arg1	effect					784:789	the synergistic effect	768:789	the synergistic effect of hydroxypropyl cellulose (HPC) and prestretching	768:840	Importantly, the synergistic effect of hydroxypropyl cellulose (HPC) and prestretching reduces the migration resistance of ions in the hydrogel, and the conductivity reaches 3.49 S/ m.
33781814	4	1	theme	excellent	713:721	arg1	strength					731:738	an excellent tensile strength	710:738	an excellent tensile strength up to 1.4 MPa	710:752	The obtained composite hydrogel has an excellent tensile strength up to 1.4 MPa.
33781814	3	2	from	embedded	475:482	arg1	hydrogel					535:542	a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel	487:542	a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel	487:542	Hydroxypropyl cellulose (HPC) biopolymer fibers were embedded in a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel, and then the prestretched PVA-HPC/SA composite hydrogel was immersed in a CaCl2 solution to prepare PVA-HPCT/SA-Ca DN hydrogels.
33781814	3	3	theme	alginate	517:524	arg1	hydrogel					535:542	a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel	487:542	a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel	487:542	Hydroxypropyl cellulose (HPC) biopolymer fibers were embedded in a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel, and then the prestretched PVA-HPC/SA composite hydrogel was immersed in a CaCl2 solution to prepare PVA-HPCT/SA-Ca DN hydrogels.
33781814	1	4	theme	due	207:209	arg1	attention					197:205	increasing attention	186:205	increasing attention due to their unique potential in artificial soft electronics	186:266	Ionic conductive hydrogels with both high-performance in conductivity and mechanical properties have received increasing attention due to their unique potential in artificial soft electronics.
33781814	2	5	with	hydrogel	326:333	arg1	conductivity					351:362	high ionic conductivity and tensile strength	340:383	conductivity	351:362	Here, a dual physically cross-linked double network (DN) hydrogel with high ionic conductivity and tensile strength was fabricated by a facile approach.
33781814	2	5	with	hydrogel	326:333	arg1	strength					376:383	high ionic conductivity and tensile strength	340:383	strength	376:383	Here, a dual physically cross-linked double network (DN) hydrogel with high ionic conductivity and tensile strength was fabricated by a facile approach.
33781814	6	6	from	noncytotoxic	983:994	arg1	addition					943:950	addition	943:950	addition	943:950	In addition, these composite hydrogels are noncytotoxic, and they have a low friction coefficient and an excellent wear resistance.
33781814	4	7	contain	has	706:708	arg1	hydrogel					697:704	The obtained composite hydrogel	674:704	The obtained composite hydrogel	674:704	The obtained composite hydrogel has an excellent tensile strength up to 1.4 MPa.
33781814	4	7	contain	has	706:708	arg2	strength					731:738	an excellent tensile strength	710:738	an excellent tensile strength up to 1.4 MPa	710:752	The obtained composite hydrogel has an excellent tensile strength up to 1.4 MPa.
33781814	3	8	theme	Hydroxypropyl	422:434	arg1	HPC					447:449	HPC	447:449	HPC	447:449	Hydroxypropyl cellulose (HPC) biopolymer fibers were embedded in a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel, and then the prestretched PVA-HPC/SA composite hydrogel was immersed in a CaCl2 solution to prepare PVA-HPCT/SA-Ca DN hydrogels.
33781814	3	8	theme	Hydroxypropyl	422:434	arg1	cellulose					436:444	Hydroxypropyl cellulose	422:444	Hydroxypropyl cellulose (HPC) biopolymer fibers	422:468	Hydroxypropyl cellulose (HPC) biopolymer fibers were embedded in a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel, and then the prestretched PVA-HPC/SA composite hydrogel was immersed in a CaCl2 solution to prepare PVA-HPCT/SA-Ca DN hydrogels.
33781814	1	9	theme	Ionic	76:80	arg1	hydrogels					93:101	Ionic conductive hydrogels	76:101	Ionic conductive hydrogels with both high-performance in conductivity and mechanical properties	76:170	Ionic conductive hydrogels with both high-performance in conductivity and mechanical properties have received increasing attention due to their unique potential in artificial soft electronics.
33781814	3	10	theme	prestretched	558:569	arg1	hydrogel					592:599	then the prestretched PVA-HPC/SA composite hydrogel	549:599	then the prestretched PVA-HPC/SA composite hydrogel	549:599	Hydroxypropyl cellulose (HPC) biopolymer fibers were embedded in a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel, and then the prestretched PVA-HPC/SA composite hydrogel was immersed in a CaCl2 solution to prepare PVA-HPCT/SA-Ca DN hydrogels.
33781814	3	11	theme	PVA-HPC/SA	571:580	arg1	hydrogel					592:599	then the prestretched PVA-HPC/SA composite hydrogel	549:599	then the prestretched PVA-HPC/SA composite hydrogel	549:599	Hydroxypropyl cellulose (HPC) biopolymer fibers were embedded in a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel, and then the prestretched PVA-HPC/SA composite hydrogel was immersed in a CaCl2 solution to prepare PVA-HPCT/SA-Ca DN hydrogels.
33781814	2	12	theme	DN	322:323	arg1	hydrogel					326:333	a dual physically cross-linked double network (DN) hydrogel	275:333	a dual physically cross-linked double network (DN) hydrogel with high ionic conductivity and tensile strength	275:383	Here, a dual physically cross-linked double network (DN) hydrogel with high ionic conductivity and tensile strength was fabricated by a facile approach.
33781814	5	13	from	hydrogel	890:897	arg1	resistance					864:873	the migration resistance	850:873	the migration resistance of ions in the hydrogel	850:897	Importantly, the synergistic effect of hydroxypropyl cellulose (HPC) and prestretching reduces the migration resistance of ions in the hydrogel, and the conductivity reaches 3.49 S/ m.
33781814	1	14	theme	conductive	82:91	arg1	hydrogels					93:101	Ionic conductive hydrogels	76:101	Ionic conductive hydrogels with both high-performance in conductivity and mechanical properties	76:170	Ionic conductive hydrogels with both high-performance in conductivity and mechanical properties have received increasing attention due to their unique potential in artificial soft electronics.
33781814	2	15	link	cross-linked	293:304	arg1	hydrogel					326:333	a dual physically cross-linked double network (DN) hydrogel	275:333	a dual physically cross-linked double network (DN) hydrogel with high ionic conductivity and tensile strength	275:383	Here, a dual physically cross-linked double network (DN) hydrogel with high ionic conductivity and tensile strength was fabricated by a facile approach.
33781814	7	16	contain	have	1111:1114	arg2	applications					1126:1137	potential applications	1116:1137	potential applications	1116:1137	Therefore, PVA-HPCT/SA-Ca DN hydrogels have potential applications in nerve replacement materials and biosensors.
33781814	7	16	contain	have	1111:1114	arg1	hydrogels					1101:1109	PVA-HPCT/SA-Ca DN hydrogels	1083:1109	PVA-HPCT/SA-Ca DN hydrogels	1083:1109	Therefore, PVA-HPCT/SA-Ca DN hydrogels have potential applications in nerve replacement materials and biosensors.
33781814	4	17	dep	1.4	746:748	arg1	to					743:744	to	743:744	to	743:744	The obtained composite hydrogel has an excellent tensile strength up to 1.4 MPa.
33781814	3	18	theme	biopolymer	452:461	arg1	fibers					463:468	Hydroxypropyl cellulose (HPC) biopolymer fibers	422:468	Hydroxypropyl cellulose (HPC) biopolymer fibers	422:468	Hydroxypropyl cellulose (HPC) biopolymer fibers were embedded in a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel, and then the prestretched PVA-HPC/SA composite hydrogel was immersed in a CaCl2 solution to prepare PVA-HPCT/SA-Ca DN hydrogels.
33781814	7	19	theme	PVA-HPCT/SA-Ca	1083:1096	arg1	hydrogels					1101:1109	PVA-HPCT/SA-Ca DN hydrogels	1083:1109	PVA-HPCT/SA-Ca DN hydrogels	1083:1109	Therefore, PVA-HPCT/SA-Ca DN hydrogels have potential applications in nerve replacement materials and biosensors.
33781814	1	20	with	hydrogels	93:101	arg1	high-performance					113:128	high-performance	113:128	high-performance	113:128	Ionic conductive hydrogels with both high-performance in conductivity and mechanical properties have received increasing attention due to their unique potential in artificial soft electronics.
33781814	1	21	from	properties	161:170	arg1	high-performance					113:128	high-performance	113:128	high-performance	113:128	Ionic conductive hydrogels with both high-performance in conductivity and mechanical properties have received increasing attention due to their unique potential in artificial soft electronics.
33781814	2	22	theme	network	313:319	arg1	hydrogel					326:333	a dual physically cross-linked double network (DN) hydrogel	275:333	a dual physically cross-linked double network (DN) hydrogel with high ionic conductivity and tensile strength	275:383	Here, a dual physically cross-linked double network (DN) hydrogel with high ionic conductivity and tensile strength was fabricated by a facile approach.
33781814	5	23	theme	ions	878:881	arg1	resistance					864:873	the migration resistance	850:873	the migration resistance of ions in the hydrogel	850:897	Importantly, the synergistic effect of hydroxypropyl cellulose (HPC) and prestretching reduces the migration resistance of ions in the hydrogel, and the conductivity reaches 3.49 S/ m.
33781814	2	24	theme	ionic	345:349	arg1	conductivity					351:362	high ionic conductivity and tensile strength	340:383	conductivity	351:362	Here, a dual physically cross-linked double network (DN) hydrogel with high ionic conductivity and tensile strength was fabricated by a facile approach.
33781814	6	25	theme	wear	1055:1058	arg1	resistance					1060:1069	an excellent wear resistance	1042:1069	an excellent wear resistance	1042:1069	In addition, these composite hydrogels are noncytotoxic, and they have a low friction coefficient and an excellent wear resistance.
33781814	1	26	theme	unique	220:225	arg1	potential					227:235	their unique potential	214:235	their unique potential in artificial soft electronics	214:266	Ionic conductive hydrogels with both high-performance in conductivity and mechanical properties have received increasing attention due to their unique potential in artificial soft electronics.
33781814	0	27	theme	Hydroxypropyl	0:12	arg1	cellulose					14:22	Hydroxypropyl cellulose	0:22	Hydroxypropyl cellulose	0:22	Hydroxypropyl cellulose enhanced ionic conductive double-network hydrogels.
33781814	7	28	theme	replacement	1148:1158	arg1	materials					1160:1168	nerve replacement materials	1142:1168	nerve replacement materials	1142:1168	Therefore, PVA-HPCT/SA-Ca DN hydrogels have potential applications in nerve replacement materials and biosensors.
33781814	2	29	theme	high	340:343	arg1	conductivity					351:362	high ionic conductivity and tensile strength	340:383	conductivity	351:362	Here, a dual physically cross-linked double network (DN) hydrogel with high ionic conductivity and tensile strength was fabricated by a facile approach.
33781814	2	30	theme	facile	405:410	arg1	approach					412:419	a facile approach	403:419	a facile approach	403:419	Here, a dual physically cross-linked double network (DN) hydrogel with high ionic conductivity and tensile strength was fabricated by a facile approach.
33781814	0	31	theme	ionic	33:37	arg1	hydrogels					65:73	ionic conductive double-network hydrogels	33:73	ionic conductive double-network hydrogels	33:73	Hydroxypropyl cellulose enhanced ionic conductive double-network hydrogels.
33781814	6	32	theme	composite	959:967	arg1	hydrogels					969:977	these composite hydrogels	953:977	these composite hydrogels	953:977	In addition, these composite hydrogels are noncytotoxic, and they have a low friction coefficient and an excellent wear resistance.
33781814	7	33	theme	potential	1116:1124	arg1	applications					1126:1137	potential applications	1116:1137	potential applications	1116:1137	Therefore, PVA-HPCT/SA-Ca DN hydrogels have potential applications in nerve replacement materials and biosensors.
33781814	5	34	theme	S/	934:935	arg1	m					937:937	3.49 S/ m	929:937	3.49 S/ m	929:937	Importantly, the synergistic effect of hydroxypropyl cellulose (HPC) and prestretching reduces the migration resistance of ions in the hydrogel, and the conductivity reaches 3.49 S/ m.
33781814	4	35	dep	strength	731:738	arg1	MPa					750:752	MPa	750:752	MPa	750:752	The obtained composite hydrogel has an excellent tensile strength up to 1.4 MPa.
33781814	3	36	theme	poly	489:492	arg1	hydrogel					535:542	a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel	487:542	a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel	487:542	Hydroxypropyl cellulose (HPC) biopolymer fibers were embedded in a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel, and then the prestretched PVA-HPC/SA composite hydrogel was immersed in a CaCl2 solution to prepare PVA-HPCT/SA-Ca DN hydrogels.
33781814	3	37	theme	composite	582:590	arg1	hydrogel					592:599	then the prestretched PVA-HPC/SA composite hydrogel	549:599	then the prestretched PVA-HPC/SA composite hydrogel	549:599	Hydroxypropyl cellulose (HPC) biopolymer fibers were embedded in a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel, and then the prestretched PVA-HPC/SA composite hydrogel was immersed in a CaCl2 solution to prepare PVA-HPCT/SA-Ca DN hydrogels.
33781814	3	38	theme	DN	660:661	arg1	hydrogels					663:671	PVA-HPCT/SA-Ca DN hydrogels	645:671	PVA-HPCT/SA-Ca DN hydrogels	645:671	Hydroxypropyl cellulose (HPC) biopolymer fibers were embedded in a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel, and then the prestretched PVA-HPC/SA composite hydrogel was immersed in a CaCl2 solution to prepare PVA-HPCT/SA-Ca DN hydrogels.
33781814	3	39	theme	‑sodium	509:515	arg1	hydrogel					535:542	a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel	487:542	a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel	487:542	Hydroxypropyl cellulose (HPC) biopolymer fibers were embedded in a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel, and then the prestretched PVA-HPC/SA composite hydrogel was immersed in a CaCl2 solution to prepare PVA-HPCT/SA-Ca DN hydrogels.
33781814	1	40	theme	artificial	240:249	arg1	electronics					256:266	artificial soft electronics	240:266	artificial soft electronics	240:266	Ionic conductive hydrogels with both high-performance in conductivity and mechanical properties have received increasing attention due to their unique potential in artificial soft electronics.
33781814	0	41	theme	double-network	50:63	arg1	hydrogels					65:73	ionic conductive double-network hydrogels	33:73	ionic conductive double-network hydrogels	33:73	Hydroxypropyl cellulose enhanced ionic conductive double-network hydrogels.
33781814	2	42	theme	tensile	368:374	arg1	strength					376:383	high ionic conductivity and tensile strength	340:383	strength	376:383	Here, a dual physically cross-linked double network (DN) hydrogel with high ionic conductivity and tensile strength was fabricated by a facile approach.
33781814	5	43	theme	migration	854:862	arg1	resistance					864:873	the migration resistance	850:873	the migration resistance of ions in the hydrogel	850:897	Importantly, the synergistic effect of hydroxypropyl cellulose (HPC) and prestretching reduces the migration resistance of ions in the hydrogel, and the conductivity reaches 3.49 S/ m.
33781814	4	44	theme	obtained	678:685	arg1	hydrogel					697:704	The obtained composite hydrogel	674:704	The obtained composite hydrogel	674:704	The obtained composite hydrogel has an excellent tensile strength up to 1.4 MPa.
33781814	1	45	theme	soft	251:254	arg1	electronics					256:266	artificial soft electronics	240:266	artificial soft electronics	240:266	Ionic conductive hydrogels with both high-performance in conductivity and mechanical properties have received increasing attention due to their unique potential in artificial soft electronics.
33781814	0	46	theme	conductive	39:48	arg1	hydrogels					65:73	ionic conductive double-network hydrogels	33:73	ionic conductive double-network hydrogels	33:73	Hydroxypropyl cellulose enhanced ionic conductive double-network hydrogels.
33781814	5	47	theme	hydroxypropyl	794:806	arg1	cellulose					808:816	hydroxypropyl cellulose	794:816	hydroxypropyl cellulose (HPC)	794:822	Importantly, the synergistic effect of hydroxypropyl cellulose (HPC) and prestretching reduces the migration resistance of ions in the hydrogel, and the conductivity reaches 3.49 S/ m.
33781814	5	47	theme	hydroxypropyl	794:806	arg1	HPC					819:821	HPC	819:821	HPC	819:821	Importantly, the synergistic effect of hydroxypropyl cellulose (HPC) and prestretching reduces the migration resistance of ions in the hydrogel, and the conductivity reaches 3.49 S/ m.
33781814	6	48	theme	friction	1017:1024	arg1	coefficient					1026:1036	a low friction coefficient	1011:1036	a low friction coefficient	1011:1036	In addition, these composite hydrogels are noncytotoxic, and they have a low friction coefficient and an excellent wear resistance.
33781814	3	49	theme	cellulose	436:444	arg1	fibers					463:468	Hydroxypropyl cellulose (HPC) biopolymer fibers	422:468	Hydroxypropyl cellulose (HPC) biopolymer fibers	422:468	Hydroxypropyl cellulose (HPC) biopolymer fibers were embedded in a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel, and then the prestretched PVA-HPC/SA composite hydrogel was immersed in a CaCl2 solution to prepare PVA-HPCT/SA-Ca DN hydrogels.
33781814	4	50	theme	composite	687:695	arg1	hydrogel					697:704	The obtained composite hydrogel	674:704	The obtained composite hydrogel	674:704	The obtained composite hydrogel has an excellent tensile strength up to 1.4 MPa.
33781814	5	51	theme	prestretching	828:840	arg1	effect					784:789	the synergistic effect	768:789	the synergistic effect of hydroxypropyl cellulose (HPC) and prestretching	768:840	Importantly, the synergistic effect of hydroxypropyl cellulose (HPC) and prestretching reduces the migration resistance of ions in the hydrogel, and the conductivity reaches 3.49 S/ m.
33781814	3	52	theme	PVA-HPCT/SA-Ca	645:658	arg1	hydrogels					663:671	PVA-HPCT/SA-Ca DN hydrogels	645:671	PVA-HPCT/SA-Ca DN hydrogels	645:671	Hydroxypropyl cellulose (HPC) biopolymer fibers were embedded in a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel, and then the prestretched PVA-HPC/SA composite hydrogel was immersed in a CaCl2 solution to prepare PVA-HPCT/SA-Ca DN hydrogels.
33781814	5	53	from	resistance	864:873	arg1	hydrogel					890:897	the hydrogel	886:897	the hydrogel	886:897	Importantly, the synergistic effect of hydroxypropyl cellulose (HPC) and prestretching reduces the migration resistance of ions in the hydrogel, and the conductivity reaches 3.49 S/ m.
33781814	7	54	theme	nerve	1142:1146	arg1	materials					1160:1168	nerve replacement materials	1142:1168	nerve replacement materials	1142:1168	Therefore, PVA-HPCT/SA-Ca DN hydrogels have potential applications in nerve replacement materials and biosensors.
33781814	6	55	theme	excellent	1045:1053	arg1	resistance					1060:1069	an excellent wear resistance	1042:1069	an excellent wear resistance	1042:1069	In addition, these composite hydrogels are noncytotoxic, and they have a low friction coefficient and an excellent wear resistance.
33781814	7	56	theme	DN	1098:1099	arg1	hydrogels					1101:1109	PVA-HPCT/SA-Ca DN hydrogels	1083:1109	PVA-HPCT/SA-Ca DN hydrogels	1083:1109	Therefore, PVA-HPCT/SA-Ca DN hydrogels have potential applications in nerve replacement materials and biosensors.
33781814	1	57	theme	mechanical	150:159	arg1	properties					161:170	mechanical properties	150:170	mechanical properties	150:170	Ionic conductive hydrogels with both high-performance in conductivity and mechanical properties have received increasing attention due to their unique potential in artificial soft electronics.
33781814	3	58	theme	CaCl2	619:623	arg1	solution					625:632	a CaCl2 solution	617:632	a CaCl2 solution	617:632	Hydroxypropyl cellulose (HPC) biopolymer fibers were embedded in a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel, and then the prestretched PVA-HPC/SA composite hydrogel was immersed in a CaCl2 solution to prepare PVA-HPCT/SA-Ca DN hydrogels.
33781814	6	59	from	addition	943:950	arg1	noncytotoxic					983:994	noncytotoxic	983:994	noncytotoxic	983:994	In addition, these composite hydrogels are noncytotoxic, and they have a low friction coefficient and an excellent wear resistance.
33781814	2	60	theme	double	306:311	arg1	hydrogel					326:333	a dual physically cross-linked double network (DN) hydrogel	275:333	a dual physically cross-linked double network (DN) hydrogel with high ionic conductivity and tensile strength	275:383	Here, a dual physically cross-linked double network (DN) hydrogel with high ionic conductivity and tensile strength was fabricated by a facile approach.
33781814	3	61	theme	vinyl	495:499	arg1	hydrogel					535:542	a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel	487:542	a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel	487:542	Hydroxypropyl cellulose (HPC) biopolymer fibers were embedded in a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel, and then the prestretched PVA-HPC/SA composite hydrogel was immersed in a CaCl2 solution to prepare PVA-HPCT/SA-Ca DN hydrogels.
33781814	1	62	from	conductivity	133:144	arg1	high-performance					113:128	high-performance	113:128	high-performance	113:128	Ionic conductive hydrogels with both high-performance in conductivity and mechanical properties have received increasing attention due to their unique potential in artificial soft electronics.
33781814	6	63	theme	low	1013:1015	arg1	coefficient					1026:1036	a low friction coefficient	1011:1036	a low friction coefficient	1011:1036	In addition, these composite hydrogels are noncytotoxic, and they have a low friction coefficient and an excellent wear resistance.
33781814	5	64	from	ions	878:881	arg1	hydrogel					890:897	the hydrogel	886:897	the hydrogel	886:897	Importantly, the synergistic effect of hydroxypropyl cellulose (HPC) and prestretching reduces the migration resistance of ions in the hydrogel, and the conductivity reaches 3.49 S/ m.
33781814	2	65	theme	cross-linked	293:304	arg1	hydrogel					326:333	a dual physically cross-linked double network (DN) hydrogel	275:333	a dual physically cross-linked double network (DN) hydrogel with high ionic conductivity and tensile strength	275:383	Here, a dual physically cross-linked double network (DN) hydrogel with high ionic conductivity and tensile strength was fabricated by a facile approach.
33781814	3	66	theme	alcohol	501:507	arg1	hydrogel					535:542	a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel	487:542	a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel	487:542	Hydroxypropyl cellulose (HPC) biopolymer fibers were embedded in a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel, and then the prestretched PVA-HPC/SA composite hydrogel was immersed in a CaCl2 solution to prepare PVA-HPCT/SA-Ca DN hydrogels.
33781814	6	67	contain	have	1006:1009	arg2	resistance					1060:1069	an excellent wear resistance	1042:1069	an excellent wear resistance	1042:1069	In addition, these composite hydrogels are noncytotoxic, and they have a low friction coefficient and an excellent wear resistance.
33781814	6	67	contain	have	1006:1009	arg2	coefficient					1026:1036	a low friction coefficient	1011:1036	a low friction coefficient	1011:1036	In addition, these composite hydrogels are noncytotoxic, and they have a low friction coefficient and an excellent wear resistance.
33781814	6	67	contain	have	1006:1009	arg1	they					1001:1004	they	1001:1004	they	1001:1004	In addition, these composite hydrogels are noncytotoxic, and they have a low friction coefficient and an excellent wear resistance.
33781814	4	68	dep	MPa	750:752	arg1	1.4					746:748	1.4	746:748	1.4	746:748	The obtained composite hydrogel has an excellent tensile strength up to 1.4 MPa.
33781814	1	69	from	potential	227:235	arg1	electronics					256:266	artificial soft electronics	240:266	artificial soft electronics	240:266	Ionic conductive hydrogels with both high-performance in conductivity and mechanical properties have received increasing attention due to their unique potential in artificial soft electronics.
33781814	3	70	theme	PVA/SA	527:532	arg1	hydrogel					535:542	a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel	487:542	a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel	487:542	Hydroxypropyl cellulose (HPC) biopolymer fibers were embedded in a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel, and then the prestretched PVA-HPC/SA composite hydrogel was immersed in a CaCl2 solution to prepare PVA-HPCT/SA-Ca DN hydrogels.
33781814	1	71	from	high-performance	113:128	arg1	properties					161:170	mechanical properties	150:170	mechanical properties	150:170	Ionic conductive hydrogels with both high-performance in conductivity and mechanical properties have received increasing attention due to their unique potential in artificial soft electronics.
33781814	1	71	from	high-performance	113:128	arg1	conductivity					133:144	conductivity	133:144	conductivity	133:144	Ionic conductive hydrogels with both high-performance in conductivity and mechanical properties have received increasing attention due to their unique potential in artificial soft electronics.
33781814	5	72	theme	cellulose	808:816	arg1	effect					784:789	the synergistic effect	768:789	the synergistic effect of hydroxypropyl cellulose (HPC) and prestretching	768:840	Importantly, the synergistic effect of hydroxypropyl cellulose (HPC) and prestretching reduces the migration resistance of ions in the hydrogel, and the conductivity reaches 3.49 S/ m.
33781814	2	73	theme	dual	277:280	arg1	hydrogel					326:333	a dual physically cross-linked double network (DN) hydrogel	275:333	a dual physically cross-linked double network (DN) hydrogel with high ionic conductivity and tensile strength	275:383	Here, a dual physically cross-linked double network (DN) hydrogel with high ionic conductivity and tensile strength was fabricated by a facile approach.
33781814	1	74	theme	increasing	186:195	arg1	attention					197:205	increasing attention	186:205	increasing attention due to their unique potential in artificial soft electronics	186:266	Ionic conductive hydrogels with both high-performance in conductivity and mechanical properties have received increasing attention due to their unique potential in artificial soft electronics.
33781814	3	75	from	hydrogel	535:542	arg1	embedded					475:482	embedded	475:482	embedded	475:482	Hydroxypropyl cellulose (HPC) biopolymer fibers were embedded in a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel, and then the prestretched PVA-HPC/SA composite hydrogel was immersed in a CaCl2 solution to prepare PVA-HPCT/SA-Ca DN hydrogels.
33781814	4	76	theme	tensile	723:729	arg1	strength					731:738	an excellent tensile strength	710:738	an excellent tensile strength up to 1.4 MPa	710:752	The obtained composite hydrogel has an excellent tensile strength up to 1.4 MPa.
33352159	4	0	theme	liver	802:806	arg1	accumulation					812:823	liver fat accumulation	802:823	liver fat accumulation	802:823	Moreover, the co-administration also restrained liver fat accumulation via affecting the expression of HMGCR and SREBP-1c genes.
33352159	1	1	theme	Sargassum	150:158	arg1	SFP					186:188	SFP	186:188	SFP	186:188	The objective of present research was to explore whether Sargassum fusiforme polysaccharide (SFP) could partly replace acarbose against type 2 diabetes in rats.
33352159	1	1	theme	Sargassum	150:158	arg1	polysaccharide					170:183	Sargassum fusiforme polysaccharide	150:183	Sargassum fusiforme polysaccharide (SFP)	150:189	The objective of present research was to explore whether Sargassum fusiforme polysaccharide (SFP) could partly replace acarbose against type 2 diabetes in rats.
33352159	5	2	theme	gut	1041:1043	arg1	flora					1045:1049	gut flora	1041:1049	gut flora	1041:1049	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	5	3	theme	Escherichia-Shigella	1263:1282	arg1	increase					1081:1088	the increase	1077:1088	the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum	1077:1240	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	5	3	theme	Escherichia-Shigella	1263:1282	arg1	decrease					1251:1258	the decrease	1247:1258	the decrease of Escherichia-Shigella	1247:1282	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	6	4	theme	drug	1418:1421	arg1	dose					1410:1413	the dose	1406:1413	the dose of drug	1406:1421	These results suggested that SFP, the novel natural adjuvant of acarbose, displayed the desirable benefits in minimizing the dose of drug, while improving the anti-diabetic efficiency.
33352159	5	5	theme	beneficial	1015:1024	arg1	composition					1026:1036	beneficial composition	1015:1036	beneficial composition of gut flora in diabetic rats	1015:1066	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	1	6	theme	fusiforme	160:168	arg1	SFP					186:188	SFP	186:188	SFP	186:188	The objective of present research was to explore whether Sargassum fusiforme polysaccharide (SFP) could partly replace acarbose against type 2 diabetes in rats.
33352159	1	6	theme	fusiforme	160:168	arg1	polysaccharide					170:183	Sargassum fusiforme polysaccharide	150:183	Sargassum fusiforme polysaccharide (SFP)	150:189	The objective of present research was to explore whether Sargassum fusiforme polysaccharide (SFP) could partly replace acarbose against type 2 diabetes in rats.
33352159	5	7	from	composition	1026:1036	arg1	rats					1063:1066	diabetic rats	1054:1066	diabetic rats	1054:1066	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	4	8	theme	SREBP-1c	867:874	arg1	expression					843:852	the expression	839:852	the expression of HMGCR and SREBP-1c genes	839:880	Moreover, the co-administration also restrained liver fat accumulation via affecting the expression of HMGCR and SREBP-1c genes.
33352159	5	9	theme	diabetic	1054:1061	arg1	rats					1063:1066	diabetic rats	1054:1066	diabetic rats	1054:1066	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	5	10	theme	Faecalibaculum	1227:1240	arg1	increase					1081:1088	the increase	1077:1088	the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum	1077:1240	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	5	10	theme	Faecalibaculum	1227:1240	arg1	decrease					1251:1258	the decrease	1247:1258	the decrease of Escherichia-Shigella	1247:1282	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	2	11	theme	serum	375:379	arg1	profiles					381:388	serum profiles	375:388	serum profiles	375:388	Results indicated that SFP co-administered with low-dose acarbose intervention typically mitigated diabetic symptoms and serum profiles and exhibited better anti-diabetic effects than single acarbose treatment in controlling fasting blood glucose, improving insulin resistance and mitigating kidney injuries.
33352159	4	12	dep	HMGCR	857:861	arg1	genes					876:880	genes	876:880	genes	876:880	Moreover, the co-administration also restrained liver fat accumulation via affecting the expression of HMGCR and SREBP-1c genes.
33352159	5	13	theme	16S	900:902	arg1	analysis					925:932	the 16S rRNA gene sequencing analysis	896:932	the 16S rRNA gene sequencing analysis	896:932	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	5	14	theme	low-dose	974:981	arg1	acarbose					983:990	low-dose acarbose	974:990	low-dose acarbose	974:990	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	0	15	from	acarbose	51:58	arg1	rats					87:90	rats	87:90	rats	87:90	Sargassum fusiforme polysaccharide partly replaces acarbose against type 2 diabetes in rats.
33352159	0	16	theme	Sargassum	0:8	arg1	polysaccharide					20:33	Sargassum fusiforme polysaccharide	0:33	Sargassum fusiforme polysaccharide	0:33	Sargassum fusiforme polysaccharide partly replaces acarbose against type 2 diabetes in rats.
33352159	2	17	theme	anti-diabetic	411:423	arg1	effects					425:431	better anti-diabetic effects	404:431	better anti-diabetic effects	404:431	Results indicated that SFP co-administered with low-dose acarbose intervention typically mitigated diabetic symptoms and serum profiles and exhibited better anti-diabetic effects than single acarbose treatment in controlling fasting blood glucose, improving insulin resistance and mitigating kidney injuries.
33352159	0	18	theme	fusiforme	10:18	arg1	polysaccharide					20:33	Sargassum fusiforme polysaccharide	0:33	Sargassum fusiforme polysaccharide	0:33	Sargassum fusiforme polysaccharide partly replaces acarbose against type 2 diabetes in rats.
33352159	5	19	theme	Lachnospiraceae	1109:1123	arg1	increase					1081:1088	the increase	1077:1088	the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum	1077:1240	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	5	19	theme	Lachnospiraceae	1109:1123	arg1	decrease					1251:1258	the decrease	1247:1258	the decrease of Escherichia-Shigella	1247:1282	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	3	20	theme	low-dose	624:631	arg1	administration					642:655	low-dose acarbose administration	624:655	low-dose acarbose administration	624:655	The RT-qPCR analysis indicated that SFP co-administered with low-dose acarbose administration distinctly activated the IRS/PI3K/AKT signaling pathway compared with single acarbose treatment.
33352159	2	21	theme	better	404:409	arg1	effects					425:431	better anti-diabetic effects	404:431	better anti-diabetic effects	404:431	Results indicated that SFP co-administered with low-dose acarbose intervention typically mitigated diabetic symptoms and serum profiles and exhibited better anti-diabetic effects than single acarbose treatment in controlling fasting blood glucose, improving insulin resistance and mitigating kidney injuries.
33352159	2	22	theme	fasting	479:485	arg1	glucose					493:499	fasting blood glucose	479:499	fasting blood glucose	479:499	Results indicated that SFP co-administered with low-dose acarbose intervention typically mitigated diabetic symptoms and serum profiles and exhibited better anti-diabetic effects than single acarbose treatment in controlling fasting blood glucose, improving insulin resistance and mitigating kidney injuries.
33352159	2	23	theme	acarbose	311:318	arg1	intervention					320:331	low-dose acarbose intervention	302:331	low-dose acarbose intervention	302:331	Results indicated that SFP co-administered with low-dose acarbose intervention typically mitigated diabetic symptoms and serum profiles and exhibited better anti-diabetic effects than single acarbose treatment in controlling fasting blood glucose, improving insulin resistance and mitigating kidney injuries.
33352159	5	24	theme	gene	909:912	arg1	analysis					925:932	the 16S rRNA gene sequencing analysis	896:932	the 16S rRNA gene sequencing analysis	896:932	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	5	25	theme	rRNA	904:907	arg1	analysis					925:932	the 16S rRNA gene sequencing analysis	896:932	the 16S rRNA gene sequencing analysis	896:932	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	2	26	theme	low-dose	302:309	arg1	intervention					320:331	low-dose acarbose intervention	302:331	low-dose acarbose intervention	302:331	Results indicated that SFP co-administered with low-dose acarbose intervention typically mitigated diabetic symptoms and serum profiles and exhibited better anti-diabetic effects than single acarbose treatment in controlling fasting blood glucose, improving insulin resistance and mitigating kidney injuries.
33352159	6	27	theme	desirable	1373:1381	arg1	benefits					1383:1390	the desirable benefits	1369:1390	the desirable benefits	1369:1390	These results suggested that SFP, the novel natural adjuvant of acarbose, displayed the desirable benefits in minimizing the dose of drug, while improving the anti-diabetic efficiency.
33352159	3	28	theme	acarbose	633:640	arg1	administration					642:655	low-dose acarbose administration	624:655	low-dose acarbose administration	624:655	The RT-qPCR analysis indicated that SFP co-administered with low-dose acarbose administration distinctly activated the IRS/PI3K/AKT signaling pathway compared with single acarbose treatment.
33352159	3	29	theme	acarbose	734:741	arg1	treatment					743:751	single acarbose treatment	727:751	single acarbose treatment	727:751	The RT-qPCR analysis indicated that SFP co-administered with low-dose acarbose administration distinctly activated the IRS/PI3K/AKT signaling pathway compared with single acarbose treatment.
33352159	2	30	theme	acarbose	445:452	arg1	treatment					454:462	single acarbose treatment	438:462	single acarbose treatment in controlling fasting blood glucose, improving insulin resistance and mitigating kidney injuries	438:560	Results indicated that SFP co-administered with low-dose acarbose intervention typically mitigated diabetic symptoms and serum profiles and exhibited better anti-diabetic effects than single acarbose treatment in controlling fasting blood glucose, improving insulin resistance and mitigating kidney injuries.
33352159	2	31	theme	insulin	512:518	arg1	resistance					520:529	insulin resistance	512:529	insulin resistance	512:529	Results indicated that SFP co-administered with low-dose acarbose intervention typically mitigated diabetic symptoms and serum profiles and exhibited better anti-diabetic effects than single acarbose treatment in controlling fasting blood glucose, improving insulin resistance and mitigating kidney injuries.
33352159	2	32	theme	diabetic	353:360	arg1	symptoms					362:369	diabetic symptoms	353:369	diabetic symptoms	353:369	Results indicated that SFP co-administered with low-dose acarbose intervention typically mitigated diabetic symptoms and serum profiles and exhibited better anti-diabetic effects than single acarbose treatment in controlling fasting blood glucose, improving insulin resistance and mitigating kidney injuries.
33352159	5	33	theme	Bifidobacterium	1126:1140	arg1	increase					1081:1088	the increase	1077:1088	the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum	1077:1240	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	5	33	theme	Bifidobacterium	1126:1140	arg1	decrease					1251:1258	the decrease	1247:1258	the decrease of Escherichia-Shigella	1247:1282	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	2	34	theme	single	438:443	arg1	treatment					454:462	single acarbose treatment	438:462	single acarbose treatment in controlling fasting blood glucose, improving insulin resistance and mitigating kidney injuries	438:560	Results indicated that SFP co-administered with low-dose acarbose intervention typically mitigated diabetic symptoms and serum profiles and exhibited better anti-diabetic effects than single acarbose treatment in controlling fasting blood glucose, improving insulin resistance and mitigating kidney injuries.
33352159	5	35	theme	Alistipes	1213:1221	arg1	increase					1081:1088	the increase	1077:1088	the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum	1077:1240	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	5	35	theme	Alistipes	1213:1221	arg1	decrease					1251:1258	the decrease	1247:1258	the decrease of Escherichia-Shigella	1247:1282	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	0	36	theme	type	68:71	arg1	diabetes					75:82	type 2 diabetes	68:82	type 2 diabetes	68:82	Sargassum fusiforme polysaccharide partly replaces acarbose against type 2 diabetes in rats.
33352159	6	37	theme	anti-diabetic	1444:1456	arg1	efficiency					1458:1467	the anti-diabetic efficiency	1440:1467	the anti-diabetic efficiency	1440:1467	These results suggested that SFP, the novel natural adjuvant of acarbose, displayed the desirable benefits in minimizing the dose of drug, while improving the anti-diabetic efficiency.
33352159	3	38	theme	single	727:732	arg1	treatment					743:751	single acarbose treatment	727:751	single acarbose treatment	727:751	The RT-qPCR analysis indicated that SFP co-administered with low-dose acarbose administration distinctly activated the IRS/PI3K/AKT signaling pathway compared with single acarbose treatment.
33352159	5	39	theme	Ruminococcaceae_UCG-014	1143:1165	arg1	increase					1081:1088	the increase	1077:1088	the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum	1077:1240	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	5	39	theme	Ruminococcaceae_UCG-014	1143:1165	arg1	decrease					1251:1258	the decrease	1247:1258	the decrease of Escherichia-Shigella	1247:1282	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	1	40	from	acarbose	212:219	arg1	rats					248:251	rats	248:251	rats	248:251	The objective of present research was to explore whether Sargassum fusiforme polysaccharide (SFP) could partly replace acarbose against type 2 diabetes in rats.
33352159	1	41	theme	present	110:116	arg1	research					118:125	present research	110:125	present research	110:125	The objective of present research was to explore whether Sargassum fusiforme polysaccharide (SFP) could partly replace acarbose against type 2 diabetes in rats.
33352159	1	42	theme	research	118:125	arg1	objective					97:105	The objective	93:105	The objective of present research	93:125	The objective of present research was to explore whether Sargassum fusiforme polysaccharide (SFP) could partly replace acarbose against type 2 diabetes in rats.
33352159	6	43	theme	acarbose	1349:1356	arg1	adjuvant					1337:1344	adjuvant	1337:1344	adjuvant	1337:1344	These results suggested that SFP, the novel natural adjuvant of acarbose, displayed the desirable benefits in minimizing the dose of drug, while improving the anti-diabetic efficiency.
33352159	6	43	theme	acarbose	1349:1356	arg1	SFP					1314:1316	SFP	1314:1316	SFP	1314:1316	These results suggested that SFP, the novel natural adjuvant of acarbose, displayed the desirable benefits in minimizing the dose of drug, while improving the anti-diabetic efficiency.
33352159	5	44	theme	flora	1045:1049	arg1	composition					1026:1036	beneficial composition	1015:1036	beneficial composition of gut flora in diabetic rats	1015:1066	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	4	45	theme	HMGCR	857:861	arg1	expression					843:852	the expression	839:852	the expression of HMGCR and SREBP-1c genes	839:880	Moreover, the co-administration also restrained liver fat accumulation via affecting the expression of HMGCR and SREBP-1c genes.
33352159	5	46	theme	Romboutsia	1184:1193	arg1	increase					1081:1088	the increase	1077:1088	the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum	1077:1240	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	5	46	theme	Romboutsia	1184:1193	arg1	decrease					1251:1258	the decrease	1247:1258	the decrease of Escherichia-Shigella	1247:1282	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	5	47	theme	sequencing	914:923	arg1	analysis					925:932	the 16S rRNA gene sequencing analysis	896:932	the 16S rRNA gene sequencing analysis	896:932	In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella.
33352159	3	48	theme	RT-qPCR	567:573	arg1	analysis					575:582	The RT-qPCR analysis	563:582	The RT-qPCR analysis	563:582	The RT-qPCR analysis indicated that SFP co-administered with low-dose acarbose administration distinctly activated the IRS/PI3K/AKT signaling pathway compared with single acarbose treatment.
33352159	1	49	theme	type	229:232	arg1	diabetes					236:243	type 2 diabetes	229:243	type 2 diabetes	229:243	The objective of present research was to explore whether Sargassum fusiforme polysaccharide (SFP) could partly replace acarbose against type 2 diabetes in rats.
33352159	2	50	theme	kidney	546:551	arg1	injuries					553:560	kidney injuries	546:560	kidney injuries	546:560	Results indicated that SFP co-administered with low-dose acarbose intervention typically mitigated diabetic symptoms and serum profiles and exhibited better anti-diabetic effects than single acarbose treatment in controlling fasting blood glucose, improving insulin resistance and mitigating kidney injuries.
33352159	3	51	theme	IRS/PI3K/AKT	682:693	arg1	pathway					705:711	the IRS/PI3K/AKT signaling pathway	678:711	the IRS/PI3K/AKT signaling pathway	678:711	The RT-qPCR analysis indicated that SFP co-administered with low-dose acarbose administration distinctly activated the IRS/PI3K/AKT signaling pathway compared with single acarbose treatment.
33352159	3	52	theme	signaling	695:703	arg1	pathway					705:711	the IRS/PI3K/AKT signaling pathway	678:711	the IRS/PI3K/AKT signaling pathway	678:711	The RT-qPCR analysis indicated that SFP co-administered with low-dose acarbose administration distinctly activated the IRS/PI3K/AKT signaling pathway compared with single acarbose treatment.
33352159	2	53	theme	blood	487:491	arg1	glucose					493:499	fasting blood glucose	479:499	fasting blood glucose	479:499	Results indicated that SFP co-administered with low-dose acarbose intervention typically mitigated diabetic symptoms and serum profiles and exhibited better anti-diabetic effects than single acarbose treatment in controlling fasting blood glucose, improving insulin resistance and mitigating kidney injuries.
33352159	4	54	theme	fat	808:810	arg1	accumulation					812:823	liver fat accumulation	802:823	liver fat accumulation	802:823	Moreover, the co-administration also restrained liver fat accumulation via affecting the expression of HMGCR and SREBP-1c genes.
32898536	7	0	theme	%	1163:1163	arg1	film					1190:1193	5 wt% CNC-reinforced composite film	1159:1193	5 wt% CNC-reinforced composite film	1159:1193	Compared with pure CMC film, an increase of 50.8% in tensile strength and a decrease of 53.4% in water vapor permeability were observed for 5 wt% CNC-reinforced composite film.
32898536	4	1	theme	optical	666:672	arg1	morphologies					652:663	the morphologies	648:663	the morphologies	648:663	The effects of CNC content on the morphologies, optical, mechanical, water vapor barrier and thermal properties of CMC/CNC films were investigated.
32898536	3	2	theme	16	582:583	arg1	index					603:607	crystallinity index	589:607	crystallinity index of 0.77	589:615	The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77.
32898536	3	2	theme	16	582:583	arg1	ratio					573:577	aspect ratio	566:577	aspect ratio of 16	566:583	The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77.
32898536	3	2	theme	16	582:583	arg1	diameter					545:552	diameter	545:552	diameter of 8-21 nm	545:563	The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77.
32898536	3	2	theme	16	582:583	arg1	length					524:529	length	524:529	length of 81-286 nm	524:542	The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77.
32898536	6	3	theme	improved	934:941	arg1	barrier					946:952	improved UV barrier	934:952	improved UV barrier	934:952	The CMC/CNC composite films showed improved UV barrier, mechanical strength, water vapor barrier and thermal stability.
32898536	4	4	theme	CNC	633:635	arg1	content					637:643	CNC content	633:643	CNC content	633:643	The effects of CNC content on the morphologies, optical, mechanical, water vapor barrier and thermal properties of CMC/CNC films were investigated.
32898536	7	5	theme	CMC	1038:1040	arg1	film					1042:1045	pure CMC film	1033:1045	pure CMC film	1033:1045	Compared with pure CMC film, an increase of 50.8% in tensile strength and a decrease of 53.4% in water vapor permeability were observed for 5 wt% CNC-reinforced composite film.
32898536	1	6	theme	available	213:221	arg1	waste					249:253	a renewable, readily available and abundant agricultural waste	192:253	a renewable, readily available and abundant agricultural waste whose high-value utilization deserves more attentions	192:307	Pea hull is a renewable, readily available and abundant agricultural waste whose high-value utilization deserves more attentions.
32898536	1	6	theme	available	213:221	arg1	hull					184:187	Pea hull	180:187	Pea hull	180:187	Pea hull is a renewable, readily available and abundant agricultural waste whose high-value utilization deserves more attentions.
32898536	4	7	theme	vapor	693:697	arg1	barrier					699:705	water vapor barrier	687:705	water vapor barrier	687:705	The effects of CNC content on the morphologies, optical, mechanical, water vapor barrier and thermal properties of CMC/CNC films were investigated.
32898536	8	8	theme	5 wt	1209:1212	arg1	film					1240:1243	5 wt% CNC-reinforced composite film	1209:1243	5 wt% CNC-reinforced composite film	1209:1243	Furthermore, 5 wt% CNC-reinforced composite film was used for red chilies packaging, which is very effective at reducing weight loss and maintaining vitamin C compared with uncoated red chilies.
32898536	0	9	theme	food	151:154	arg1	applications					166:177	food packaging applications	151:177	food packaging applications	151:177	Preparation and characterization of carboxymethyl cellulose-based composite films reinforced by cellulose nanocrystals derived from pea hull waste for food packaging applications.
32898536	1	10	theme	abundant	227:234	arg1	waste					249:253	a renewable, readily available and abundant agricultural waste	192:253	a renewable, readily available and abundant agricultural waste whose high-value utilization deserves more attentions	192:307	Pea hull is a renewable, readily available and abundant agricultural waste whose high-value utilization deserves more attentions.
32898536	1	10	theme	abundant	227:234	arg1	hull					184:187	Pea hull	180:187	Pea hull	180:187	Pea hull is a renewable, readily available and abundant agricultural waste whose high-value utilization deserves more attentions.
32898536	6	11	theme	CMC/CNC	903:909	arg1	films					921:925	The CMC/CNC composite films	899:925	The CMC/CNC composite films	899:925	The CMC/CNC composite films showed improved UV barrier, mechanical strength, water vapor barrier and thermal stability.
32898536	8	12	theme	red	1378:1380	arg1	chilies					1382:1388	uncoated red chilies	1369:1388	uncoated red chilies	1369:1388	Furthermore, 5 wt% CNC-reinforced composite film was used for red chilies packaging, which is very effective at reducing weight loss and maintaining vitamin C compared with uncoated red chilies.
32898536	8	13	theme	CNC-reinforced	1215:1228	arg1	film					1240:1243	5 wt% CNC-reinforced composite film	1209:1243	5 wt% CNC-reinforced composite film	1209:1243	Furthermore, 5 wt% CNC-reinforced composite film was used for red chilies packaging, which is very effective at reducing weight loss and maintaining vitamin C compared with uncoated red chilies.
32898536	3	14	theme	crystallinity	589:601	arg1	index					603:607	crystallinity index	589:607	crystallinity index of 0.77	589:615	The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77.
32898536	4	15	theme	thermal	711:717	arg1	properties					719:728	thermal properties	711:728	thermal properties of CMC/CNC films	711:745	The effects of CNC content on the morphologies, optical, mechanical, water vapor barrier and thermal properties of CMC/CNC films were investigated.
32898536	9	16	theme	composite	1432:1440	arg1	film					1442:1445	the CMC/CNC composite film	1420:1445	the CMC/CNC composite film	1420:1445	These results indicated that the CMC/CNC composite film may have promising application potential as edible food packaging material.
32898536	6	17	theme	vapor	982:986	arg1	barrier					988:994	water vapor barrier	976:994	water vapor barrier	976:994	The CMC/CNC composite films showed improved UV barrier, mechanical strength, water vapor barrier and thermal stability.
32898536	3	18	theme	obtained	477:484	arg1	CNC					486:488	The obtained CNC	473:488	The obtained CNC	473:488	The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77.
32898536	7	19	theme	5 wt	1159:1162	arg1	film					1190:1193	5 wt% CNC-reinforced composite film	1159:1193	5 wt% CNC-reinforced composite film	1159:1193	Compared with pure CMC film, an increase of 50.8% in tensile strength and a decrease of 53.4% in water vapor permeability were observed for 5 wt% CNC-reinforced composite film.
32898536	8	20	used	used	1249:1252	arg2	film					1240:1243	5 wt% CNC-reinforced composite film	1209:1243	5 wt% CNC-reinforced composite film	1209:1243	Furthermore, 5 wt% CNC-reinforced composite film was used for red chilies packaging, which is very effective at reducing weight loss and maintaining vitamin C compared with uncoated red chilies.
32898536	8	21	theme	red	1258:1260	arg1	packaging					1270:1278	red chilies packaging	1258:1278	red chilies packaging	1258:1278	Furthermore, 5 wt% CNC-reinforced composite film was used for red chilies packaging, which is very effective at reducing weight loss and maintaining vitamin C compared with uncoated red chilies.
32898536	0	22	theme	cellulose	96:104	arg1	nanocrystals					106:117	cellulose nanocrystals	96:117	cellulose nanocrystals derived from pea hull waste for food packaging applications	96:177	Preparation and characterization of carboxymethyl cellulose-based composite films reinforced by cellulose nanocrystals derived from pea hull waste for food packaging applications.
32898536	9	23	theme	promising	1456:1464	arg1	potential					1478:1486	promising application potential	1456:1486	promising application potential	1456:1486	These results indicated that the CMC/CNC composite film may have promising application potential as edible food packaging material.
32898536	6	24	theme	mechanical	955:964	arg1	strength					966:973	mechanical strength	955:973	mechanical strength	955:973	The CMC/CNC composite films showed improved UV barrier, mechanical strength, water vapor barrier and thermal stability.
32898536	2	25	theme	reinforcement	408:420	arg1	capability					422:431	its reinforcement capability	404:431	its reinforcement capability for carboxymethyl cellulose (CMC) film	404:470	This work aimed at the isolation of cellulose nanocrystals (CNC) from pea hull and evaluation its reinforcement capability for carboxymethyl cellulose (CMC) film.
32898536	7	26	theme	water	1116:1120	arg1	permeability					1128:1139	water vapor permeability	1116:1139	water vapor permeability	1116:1139	Compared with pure CMC film, an increase of 50.8% in tensile strength and a decrease of 53.4% in water vapor permeability were observed for 5 wt% CNC-reinforced composite film.
32898536	9	27	theme	edible	1491:1496	arg1	material					1513:1520	edible food packaging material	1491:1520	edible food packaging material	1491:1520	These results indicated that the CMC/CNC composite film may have promising application potential as edible food packaging material.
32898536	7	28	theme	%	1111:1111	arg1	increase					1051:1058	an increase	1048:1058	an increase of 50.8% in tensile strength	1048:1087	Compared with pure CMC film, an increase of 50.8% in tensile strength and a decrease of 53.4% in water vapor permeability were observed for 5 wt% CNC-reinforced composite film.
32898536	7	28	theme	%	1111:1111	arg1	decrease					1095:1102	a decrease	1093:1102	a decrease of 53.4% in water vapor permeability	1093:1139	Compared with pure CMC film, an increase of 50.8% in tensile strength and a decrease of 53.4% in water vapor permeability were observed for 5 wt% CNC-reinforced composite film.
32898536	9	29	theme	packaging	1503:1511	arg1	material					1513:1520	edible food packaging material	1491:1520	edible food packaging material	1491:1520	These results indicated that the CMC/CNC composite film may have promising application potential as edible food packaging material.
32898536	2	30	theme	CMC	462:464	arg1	film					467:470	carboxymethyl cellulose (CMC) film	437:470	carboxymethyl cellulose (CMC) film	437:470	This work aimed at the isolation of cellulose nanocrystals (CNC) from pea hull and evaluation its reinforcement capability for carboxymethyl cellulose (CMC) film.
32898536	4	31	theme	films	741:745	arg1	barrier					699:705	water vapor barrier	687:705	water vapor barrier	687:705	The effects of CNC content on the morphologies, optical, mechanical, water vapor barrier and thermal properties of CMC/CNC films were investigated.
32898536	4	31	theme	films	741:745	arg1	properties					719:728	thermal properties	711:728	thermal properties of CMC/CNC films	711:745	The effects of CNC content on the morphologies, optical, mechanical, water vapor barrier and thermal properties of CMC/CNC films were investigated.
32898536	4	31	theme	films	741:745	arg1	effects					622:628	The effects	618:628	The effects of CNC content on the morphologies, optical, mechanical	618:684	The effects of CNC content on the morphologies, optical, mechanical, water vapor barrier and thermal properties of CMC/CNC films were investigated.
32898536	2	32	theme	cellulose	451:459	arg1	film					467:470	carboxymethyl cellulose (CMC) film	437:470	carboxymethyl cellulose (CMC) film	437:470	This work aimed at the isolation of cellulose nanocrystals (CNC) from pea hull and evaluation its reinforcement capability for carboxymethyl cellulose (CMC) film.
32898536	7	33	from	increase	1051:1058	arg1	permeability					1128:1139	water vapor permeability	1116:1139	water vapor permeability	1116:1139	Compared with pure CMC film, an increase of 50.8% in tensile strength and a decrease of 53.4% in water vapor permeability were observed for 5 wt% CNC-reinforced composite film.
32898536	7	33	from	increase	1051:1058	arg1	strength					1080:1087	tensile strength	1072:1087	tensile strength	1072:1087	Compared with pure CMC film, an increase of 50.8% in tensile strength and a decrease of 53.4% in water vapor permeability were observed for 5 wt% CNC-reinforced composite film.
32898536	8	34	theme	weight	1317:1322	arg1	loss					1324:1327	weight loss	1317:1327	weight loss	1317:1327	Furthermore, 5 wt% CNC-reinforced composite film was used for red chilies packaging, which is very effective at reducing weight loss and maintaining vitamin C compared with uncoated red chilies.
32898536	0	35	theme	carboxymethyl	36:48	arg1	films					76:80	carboxymethyl cellulose-based composite films	36:80	carboxymethyl cellulose-based composite films	36:80	Preparation and characterization of carboxymethyl cellulose-based composite films reinforced by cellulose nanocrystals derived from pea hull waste for food packaging applications.
32898536	2	36	from	evaluation	393:402	arg1	isolation					333:341	the isolation	329:341	the isolation of cellulose nanocrystals (CNC) from pea hull and evaluation	329:402	This work aimed at the isolation of cellulose nanocrystals (CNC) from pea hull and evaluation its reinforcement capability for carboxymethyl cellulose (CMC) film.
32898536	0	37	theme	composite	66:74	arg1	films					76:80	carboxymethyl cellulose-based composite films	36:80	carboxymethyl cellulose-based composite films	36:80	Preparation and characterization of carboxymethyl cellulose-based composite films reinforced by cellulose nanocrystals derived from pea hull waste for food packaging applications.
32898536	8	38	theme	vitamin	1345:1351	arg1	C					1353:1353	vitamin C	1345:1353	vitamin C	1345:1353	Furthermore, 5 wt% CNC-reinforced composite film was used for red chilies packaging, which is very effective at reducing weight loss and maintaining vitamin C compared with uncoated red chilies.
32898536	2	39	theme	nanocrystals	356:367	arg1	isolation					333:341	the isolation	329:341	the isolation of cellulose nanocrystals (CNC) from pea hull and evaluation	329:402	This work aimed at the isolation of cellulose nanocrystals (CNC) from pea hull and evaluation its reinforcement capability for carboxymethyl cellulose (CMC) film.
32898536	1	40	theme	agricultural	236:247	arg1	waste					249:253	a renewable, readily available and abundant agricultural waste	192:253	a renewable, readily available and abundant agricultural waste whose high-value utilization deserves more attentions	192:307	Pea hull is a renewable, readily available and abundant agricultural waste whose high-value utilization deserves more attentions.
32898536	1	40	theme	agricultural	236:247	arg1	hull					184:187	Pea hull	180:187	Pea hull	180:187	Pea hull is a renewable, readily available and abundant agricultural waste whose high-value utilization deserves more attentions.
32898536	0	41	attach	derived	119:125	arg1	waste					141:145	pea hull waste	132:145	pea hull waste for food packaging applications	132:177	Preparation and characterization of carboxymethyl cellulose-based composite films reinforced by cellulose nanocrystals derived from pea hull waste for food packaging applications.
32898536	0	41	attach	derived	119:125	arg2	nanocrystals					106:117	cellulose nanocrystals	96:117	cellulose nanocrystals derived from pea hull waste for food packaging applications	96:177	Preparation and characterization of carboxymethyl cellulose-based composite films reinforced by cellulose nanocrystals derived from pea hull waste for food packaging applications.
32898536	3	42	theme	81-286 nm	534:542	arg1	index					603:607	crystallinity index	589:607	crystallinity index of 0.77	589:615	The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77.
32898536	3	42	theme	81-286 nm	534:542	arg1	ratio					573:577	aspect ratio	566:577	aspect ratio of 16	566:583	The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77.
32898536	3	42	theme	81-286 nm	534:542	arg1	diameter					545:552	diameter	545:552	diameter of 8-21 nm	545:563	The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77.
32898536	3	42	theme	81-286 nm	534:542	arg1	length					524:529	length	524:529	length of 81-286 nm	524:542	The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77.
32898536	0	43	theme	films	76:80	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of carboxymethyl cellulose-based composite films reinforced by cellulose nanocrystals derived from pea hull waste for food packaging applications.
32898536	0	43	theme	films	76:80	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of carboxymethyl cellulose-based composite films reinforced by cellulose nanocrystals derived from pea hull waste for food packaging applications.
32898536	7	44	theme	%	1067:1067	arg1	increase					1051:1058	an increase	1048:1058	an increase of 50.8% in tensile strength	1048:1087	Compared with pure CMC film, an increase of 50.8% in tensile strength and a decrease of 53.4% in water vapor permeability were observed for 5 wt% CNC-reinforced composite film.
32898536	7	44	theme	%	1067:1067	arg1	decrease					1095:1102	a decrease	1093:1102	a decrease of 53.4% in water vapor permeability	1093:1139	Compared with pure CMC film, an increase of 50.8% in tensile strength and a decrease of 53.4% in water vapor permeability were observed for 5 wt% CNC-reinforced composite film.
32898536	7	45	theme	CNC-reinforced	1165:1178	arg1	film					1190:1193	5 wt% CNC-reinforced composite film	1159:1193	5 wt% CNC-reinforced composite film	1159:1193	Compared with pure CMC film, an increase of 50.8% in tensile strength and a decrease of 53.4% in water vapor permeability were observed for 5 wt% CNC-reinforced composite film.
32898536	3	46	theme	8-21 nm	557:563	arg1	index					603:607	crystallinity index	589:607	crystallinity index of 0.77	589:615	The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77.
32898536	3	46	theme	8-21 nm	557:563	arg1	ratio					573:577	aspect ratio	566:577	aspect ratio of 16	566:583	The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77.
32898536	3	46	theme	8-21 nm	557:563	arg1	diameter					545:552	diameter	545:552	diameter of 8-21 nm	545:563	The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77.
32898536	3	46	theme	8-21 nm	557:563	arg1	length					524:529	length	524:529	length of 81-286 nm	524:542	The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77.
32898536	1	47	theme	more	293:296	arg1	attentions					298:307	more attentions	293:307	more attentions	293:307	Pea hull is a renewable, readily available and abundant agricultural waste whose high-value utilization deserves more attentions.
32898536	4	48	theme	water	687:691	arg1	barrier					699:705	water vapor barrier	687:705	water vapor barrier	687:705	The effects of CNC content on the morphologies, optical, mechanical, water vapor barrier and thermal properties of CMC/CNC films were investigated.
32898536	5	49	theme	homogenous	846:855	arg1	films					857:861	homogenous films	846:861	homogenous films	846:861	SEM images showed that the CNC was evenly distributed in the CMC matrix to form homogenous films when the content of CNC was ≤5 wt%.
32898536	5	50	theme	SEM	766:768	arg1	images					770:775	SEM images	766:775	SEM images	766:775	SEM images showed that the CNC was evenly distributed in the CMC matrix to form homogenous films when the content of CNC was ≤5 wt%.
32898536	1	51	theme	renewable	194:202	arg1	waste					249:253	a renewable, readily available and abundant agricultural waste	192:253	a renewable, readily available and abundant agricultural waste whose high-value utilization deserves more attentions	192:307	Pea hull is a renewable, readily available and abundant agricultural waste whose high-value utilization deserves more attentions.
32898536	1	51	theme	renewable	194:202	arg1	hull					184:187	Pea hull	180:187	Pea hull	180:187	Pea hull is a renewable, readily available and abundant agricultural waste whose high-value utilization deserves more attentions.
32898536	6	52	theme	UV	943:944	arg1	barrier					946:952	improved UV barrier	934:952	improved UV barrier	934:952	The CMC/CNC composite films showed improved UV barrier, mechanical strength, water vapor barrier and thermal stability.
32898536	2	53	from	hull	384:387	arg1	isolation					333:341	the isolation	329:341	the isolation of cellulose nanocrystals (CNC) from pea hull and evaluation	329:402	This work aimed at the isolation of cellulose nanocrystals (CNC) from pea hull and evaluation its reinforcement capability for carboxymethyl cellulose (CMC) film.
32898536	7	54	theme	pure	1033:1036	arg1	film					1042:1045	pure CMC film	1033:1045	pure CMC film	1033:1045	Compared with pure CMC film, an increase of 50.8% in tensile strength and a decrease of 53.4% in water vapor permeability were observed for 5 wt% CNC-reinforced composite film.
32898536	3	55	theme	aspect	566:571	arg1	ratio					573:577	aspect ratio	566:577	aspect ratio of 16	566:583	The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77.
32898536	8	56	theme	uncoated	1369:1376	arg1	chilies					1382:1388	uncoated red chilies	1369:1388	uncoated red chilies	1369:1388	Furthermore, 5 wt% CNC-reinforced composite film was used for red chilies packaging, which is very effective at reducing weight loss and maintaining vitamin C compared with uncoated red chilies.
32898536	0	57	theme	packaging	156:164	arg1	applications					166:177	food packaging applications	151:177	food packaging applications	151:177	Preparation and characterization of carboxymethyl cellulose-based composite films reinforced by cellulose nanocrystals derived from pea hull waste for food packaging applications.
32898536	5	58	theme	CMC	827:829	arg1	matrix					831:836	the CMC matrix	823:836	the CMC matrix	823:836	SEM images showed that the CNC was evenly distributed in the CMC matrix to form homogenous films when the content of CNC was ≤5 wt%.
32898536	6	59	theme	composite	911:919	arg1	films					921:925	The CMC/CNC composite films	899:925	The CMC/CNC composite films	899:925	The CMC/CNC composite films showed improved UV barrier, mechanical strength, water vapor barrier and thermal stability.
32898536	4	60	theme	content	637:643	arg1	barrier					699:705	water vapor barrier	687:705	water vapor barrier	687:705	The effects of CNC content on the morphologies, optical, mechanical, water vapor barrier and thermal properties of CMC/CNC films were investigated.
32898536	4	60	theme	content	637:643	arg1	properties					719:728	thermal properties	711:728	thermal properties of CMC/CNC films	711:745	The effects of CNC content on the morphologies, optical, mechanical, water vapor barrier and thermal properties of CMC/CNC films were investigated.
32898536	4	60	theme	content	637:643	arg1	effects					622:628	The effects	618:628	The effects of CNC content on the morphologies, optical, mechanical	618:684	The effects of CNC content on the morphologies, optical, mechanical, water vapor barrier and thermal properties of CMC/CNC films were investigated.
32898536	5	61	theme	CNC	883:885	arg1	content					872:878	the content	868:878	the content of CNC	868:885	SEM images showed that the CNC was evenly distributed in the CMC matrix to form homogenous films when the content of CNC was ≤5 wt%.
32898536	5	61	theme	CNC	883:885	arg1	%					896:896	≤5 wt%	891:896	≤5 wt%	891:896	SEM images showed that the CNC was evenly distributed in the CMC matrix to form homogenous films when the content of CNC was ≤5 wt%.
32898536	8	62	theme	%	1213:1213	arg1	film					1240:1243	5 wt% CNC-reinforced composite film	1209:1243	5 wt% CNC-reinforced composite film	1209:1243	Furthermore, 5 wt% CNC-reinforced composite film was used for red chilies packaging, which is very effective at reducing weight loss and maintaining vitamin C compared with uncoated red chilies.
32898536	9	63	theme	CMC/CNC	1424:1430	arg1	film					1442:1445	the CMC/CNC composite film	1420:1445	the CMC/CNC composite film	1420:1445	These results indicated that the CMC/CNC composite film may have promising application potential as edible food packaging material.
32898536	8	64	theme	composite	1230:1238	arg1	film					1240:1243	5 wt% CNC-reinforced composite film	1209:1243	5 wt% CNC-reinforced composite film	1209:1243	Furthermore, 5 wt% CNC-reinforced composite film was used for red chilies packaging, which is very effective at reducing weight loss and maintaining vitamin C compared with uncoated red chilies.
32898536	4	65	from	effects	622:628	arg1	morphologies					652:663	the morphologies	648:663	the morphologies	648:663	The effects of CNC content on the morphologies, optical, mechanical, water vapor barrier and thermal properties of CMC/CNC films were investigated.
32898536	3	66	theme	0.77	612:615	arg1	index					603:607	crystallinity index	589:607	crystallinity index of 0.77	589:615	The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77.
32898536	3	66	theme	0.77	612:615	arg1	ratio					573:577	aspect ratio	566:577	aspect ratio of 16	566:583	The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77.
32898536	3	66	theme	0.77	612:615	arg1	diameter					545:552	diameter	545:552	diameter of 8-21 nm	545:563	The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77.
32898536	3	66	theme	0.77	612:615	arg1	length					524:529	length	524:529	length of 81-286 nm	524:542	The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77.
32898536	6	67	theme	water	976:980	arg1	barrier					988:994	water vapor barrier	976:994	water vapor barrier	976:994	The CMC/CNC composite films showed improved UV barrier, mechanical strength, water vapor barrier and thermal stability.
32898536	1	68	theme	Pea	180:182	arg1	waste					249:253	a renewable, readily available and abundant agricultural waste	192:253	a renewable, readily available and abundant agricultural waste whose high-value utilization deserves more attentions	192:307	Pea hull is a renewable, readily available and abundant agricultural waste whose high-value utilization deserves more attentions.
32898536	1	68	theme	Pea	180:182	arg1	hull					184:187	Pea hull	180:187	Pea hull	180:187	Pea hull is a renewable, readily available and abundant agricultural waste whose high-value utilization deserves more attentions.
32898536	7	69	theme	vapor	1122:1126	arg1	permeability					1128:1139	water vapor permeability	1116:1139	water vapor permeability	1116:1139	Compared with pure CMC film, an increase of 50.8% in tensile strength and a decrease of 53.4% in water vapor permeability were observed for 5 wt% CNC-reinforced composite film.
32898536	9	70	theme	application	1466:1476	arg1	potential					1478:1486	promising application potential	1456:1486	promising application potential	1456:1486	These results indicated that the CMC/CNC composite film may have promising application potential as edible food packaging material.
32898536	7	71	from	decrease	1095:1102	arg1	permeability					1128:1139	water vapor permeability	1116:1139	water vapor permeability	1116:1139	Compared with pure CMC film, an increase of 50.8% in tensile strength and a decrease of 53.4% in water vapor permeability were observed for 5 wt% CNC-reinforced composite film.
32898536	7	71	from	decrease	1095:1102	arg1	strength					1080:1087	tensile strength	1072:1087	tensile strength	1072:1087	Compared with pure CMC film, an increase of 50.8% in tensile strength and a decrease of 53.4% in water vapor permeability were observed for 5 wt% CNC-reinforced composite film.
32898536	8	72	theme	chilies	1262:1268	arg1	packaging					1270:1278	red chilies packaging	1258:1278	red chilies packaging	1258:1278	Furthermore, 5 wt% CNC-reinforced composite film was used for red chilies packaging, which is very effective at reducing weight loss and maintaining vitamin C compared with uncoated red chilies.
32898536	2	73	theme	cellulose	346:354	arg1	CNC					370:372	CNC	370:372	CNC	370:372	This work aimed at the isolation of cellulose nanocrystals (CNC) from pea hull and evaluation its reinforcement capability for carboxymethyl cellulose (CMC) film.
32898536	2	73	theme	cellulose	346:354	arg1	nanocrystals					356:367	cellulose nanocrystals	346:367	cellulose nanocrystals (CNC)	346:373	This work aimed at the isolation of cellulose nanocrystals (CNC) from pea hull and evaluation its reinforcement capability for carboxymethyl cellulose (CMC) film.
32898536	0	74	theme	hull	136:139	arg1	waste					141:145	pea hull waste	132:145	pea hull waste for food packaging applications	132:177	Preparation and characterization of carboxymethyl cellulose-based composite films reinforced by cellulose nanocrystals derived from pea hull waste for food packaging applications.
32898536	9	75	theme	food	1498:1501	arg1	material					1513:1520	edible food packaging material	1491:1520	edible food packaging material	1491:1520	These results indicated that the CMC/CNC composite film may have promising application potential as edible food packaging material.
32898536	4	76	from	properties	719:728	arg1	morphologies					652:663	the morphologies	648:663	the morphologies	648:663	The effects of CNC content on the morphologies, optical, mechanical, water vapor barrier and thermal properties of CMC/CNC films were investigated.
32898536	4	77	theme	CMC/CNC	733:739	arg1	films					741:745	CMC/CNC films	733:745	CMC/CNC films	733:745	The effects of CNC content on the morphologies, optical, mechanical, water vapor barrier and thermal properties of CMC/CNC films were investigated.
32898536	2	78	theme	carboxymethyl	437:449	arg1	film					467:470	carboxymethyl cellulose (CMC) film	437:470	carboxymethyl cellulose (CMC) film	437:470	This work aimed at the isolation of cellulose nanocrystals (CNC) from pea hull and evaluation its reinforcement capability for carboxymethyl cellulose (CMC) film.
32898536	0	79	theme	cellulose-based	50:64	arg1	films					76:80	carboxymethyl cellulose-based composite films	36:80	carboxymethyl cellulose-based composite films	36:80	Preparation and characterization of carboxymethyl cellulose-based composite films reinforced by cellulose nanocrystals derived from pea hull waste for food packaging applications.
32898536	6	80	theme	thermal	1000:1006	arg1	stability					1008:1016	thermal stability	1000:1016	thermal stability	1000:1016	The CMC/CNC composite films showed improved UV barrier, mechanical strength, water vapor barrier and thermal stability.
32898536	0	81	theme	pea	132:134	arg1	waste					141:145	pea hull waste	132:145	pea hull waste for food packaging applications	132:177	Preparation and characterization of carboxymethyl cellulose-based composite films reinforced by cellulose nanocrystals derived from pea hull waste for food packaging applications.
32898536	7	82	theme	tensile	1072:1078	arg1	strength					1080:1087	tensile strength	1072:1087	tensile strength	1072:1087	Compared with pure CMC film, an increase of 50.8% in tensile strength and a decrease of 53.4% in water vapor permeability were observed for 5 wt% CNC-reinforced composite film.
32898536	9	83	contain	have	1451:1454	arg2	potential					1478:1486	promising application potential	1456:1486	promising application potential	1456:1486	These results indicated that the CMC/CNC composite film may have promising application potential as edible food packaging material.
32898536	9	83	contain	have	1451:1454	arg1	film					1442:1445	the CMC/CNC composite film	1420:1445	the CMC/CNC composite film	1420:1445	These results indicated that the CMC/CNC composite film may have promising application potential as edible food packaging material.
32898536	4	84	dep	optical	666:672	arg1	mechanical					675:684	mechanical	675:684	mechanical	675:684	The effects of CNC content on the morphologies, optical, mechanical, water vapor barrier and thermal properties of CMC/CNC films were investigated.
32898536	3	85	theme	needle-like	500:510	arg1	shapes					512:517	needle-like shapes	500:517	needle-like shapes	500:517	The obtained CNC displayed needle-like shapes with length of 81-286 nm, diameter of 8-21 nm, aspect ratio of 16 and crystallinity index of 0.77.
32898536	4	86	from	barrier	699:705	arg1	morphologies					652:663	the morphologies	648:663	the morphologies	648:663	The effects of CNC content on the morphologies, optical, mechanical, water vapor barrier and thermal properties of CMC/CNC films were investigated.
32898536	7	87	theme	composite	1180:1188	arg1	film					1190:1193	5 wt% CNC-reinforced composite film	1159:1193	5 wt% CNC-reinforced composite film	1159:1193	Compared with pure CMC film, an increase of 50.8% in tensile strength and a decrease of 53.4% in water vapor permeability were observed for 5 wt% CNC-reinforced composite film.
32898536	2	88	theme	pea	380:382	arg1	hull					384:387	pea hull	380:387	pea hull	380:387	This work aimed at the isolation of cellulose nanocrystals (CNC) from pea hull and evaluation its reinforcement capability for carboxymethyl cellulose (CMC) film.
32898536	1	89	theme	high-value	261:270	arg1	utilization					272:282	utilization	272:282	utilization	272:282	Pea hull is a renewable, readily available and abundant agricultural waste whose high-value utilization deserves more attentions.
32588539	9	0	from	BGN	1205:1207	arg1	matrix					1219:1224	the CH matrix	1212:1224	the CH matrix	1212:1224	Obtained results showed a macroporous morphology of the scaffold with a pore size of about 200 μm, and a homogeneous distribution of the BGN in the CH matrix.
32588539	13	1	dep	in	1508:1509	arg1	vitro					1511:1515	vitro	1511:1515	vitro	1511:1515	The in vitro bioactivity studies confirmed the formation of an apatite layer on the surface of the hybrid scaffold, which results in a direct bone bonding of the implant.
32588539	9	2	theme	pore	1140:1143	arg1	size					1145:1148	a pore size	1138:1148	a pore size of about 200 μm	1138:1164	Obtained results showed a macroporous morphology of the scaffold with a pore size of about 200 μm, and a homogeneous distribution of the BGN in the CH matrix.
32588539	13	3	from	surface	1588:1594	arg1	formation					1551:1559	the formation	1547:1559	the formation of an apatite layer on the surface of the hybrid scaffold, which results in a direct bone bonding of the implant	1547:1672	The in vitro bioactivity studies confirmed the formation of an apatite layer on the surface of the hybrid scaffold, which results in a direct bone bonding of the implant.
32588539	9	4	theme	200 μm	1159:1164	arg1	size					1145:1148	a pore size	1138:1148	a pore size of about 200 μm	1138:1164	Obtained results showed a macroporous morphology of the scaffold with a pore size of about 200 μm, and a homogeneous distribution of the BGN in the CH matrix.
32588539	2	5	theme	engineering	282:292	arg1	purposes					294:301	tissue engineering purposes	275:301	tissue engineering purposes	275:301	A number of biodegradable polymers have been explored for tissue engineering purposes.
32588539	12	6	dep	in	1380:1381	arg1	vitro					1383:1387	vitro	1383:1387	vitro	1383:1387	The in vitro assays showed adequate degradation properties, which is essential for the potential replacement by the new tissue.
32588539	5	7	theme	acetic	636:641	arg1	acid					643:646	acetic acid	636:646	acetic acid	636:646	This method has been studied by adjusting the concentration of acetic acid; this process can influence the structure properties of the scaffold.
32588539	1	8	theme	osseous	200:206	arg1	defects					208:214	osseous defects	200:214	osseous defects	200:214	Bone tissue engineering is gaining popularity as an alternative method for the treatment of osseous defects.
32588539	9	9	from	morphology	1106:1115	arg1	matrix					1219:1224	the CH matrix	1212:1224	the CH matrix	1212:1224	Obtained results showed a macroporous morphology of the scaffold with a pore size of about 200 μm, and a homogeneous distribution of the BGN in the CH matrix.
32588539	9	10	theme	homogeneous	1173:1183	arg1	distribution					1185:1196	a homogeneous distribution	1171:1196	a homogeneous distribution of the BGN in the CH matrix	1171:1224	Obtained results showed a macroporous morphology of the scaffold with a pore size of about 200 μm, and a homogeneous distribution of the BGN in the CH matrix.
32588539	4	11	theme	hybrid	456:461	arg1	scaffold					463:470	a hybrid scaffold	454:470	a hybrid scaffold of chitosan (CH) and bioactive glass nanoparticles (BGN)	454:527	In this work, a hybrid scaffold of chitosan (CH) and bioactive glass nanoparticles (BGN) was prepared by the freeze-gelation method.
32588539	5	12	theme	scaffold	708:715	arg1	properties					690:699	the structure properties	676:699	the structure properties of the scaffold	676:715	This method has been studied by adjusting the concentration of acetic acid; this process can influence the structure properties of the scaffold.
32588539	2	13	theme	polymers	243:250	arg1	number					219:224	A number	217:224	A number of biodegradable polymers	217:250	A number of biodegradable polymers have been explored for tissue engineering purposes.
32588539	9	14	from	matrix	1219:1224	arg1	morphology					1106:1115	a macroporous morphology	1092:1115	a macroporous morphology of the scaffold with a pore size of about 200 μm	1092:1164	Obtained results showed a macroporous morphology of the scaffold with a pore size of about 200 μm, and a homogeneous distribution of the BGN in the CH matrix.
32588539	9	14	from	matrix	1219:1224	arg1	distribution					1185:1196	a homogeneous distribution	1171:1196	a homogeneous distribution of the BGN in the CH matrix	1171:1224	Obtained results showed a macroporous morphology of the scaffold with a pore size of about 200 μm, and a homogeneous distribution of the BGN in the CH matrix.
32588539	9	15	theme	BGN	1205:1207	arg1	morphology					1106:1115	a macroporous morphology	1092:1115	a macroporous morphology of the scaffold with a pore size of about 200 μm	1092:1164	Obtained results showed a macroporous morphology of the scaffold with a pore size of about 200 μm, and a homogeneous distribution of the BGN in the CH matrix.
32588539	9	15	theme	BGN	1205:1207	arg1	distribution					1185:1196	a homogeneous distribution	1171:1196	a homogeneous distribution of the BGN in the CH matrix	1171:1224	Obtained results showed a macroporous morphology of the scaffold with a pore size of about 200 μm, and a homogeneous distribution of the BGN in the CH matrix.
32588539	3	16	theme	new	306:308	arg1	family					310:315	A new family	304:315	A new family of biodegradable polymer/bioactive glass composite materials	304:376	A new family of biodegradable polymer/bioactive glass composite materials has been designed to be used in bone regeneration approaches.
32588539	5	17	theme	structure	680:688	arg1	properties					690:699	the structure properties	676:699	the structure properties of the scaffold	676:715	This method has been studied by adjusting the concentration of acetic acid; this process can influence the structure properties of the scaffold.
32588539	14	18	theme	tissue	1770:1775	arg1	engineering					1777:1787	bone tissue engineering	1765:1787	bone tissue engineering	1765:1787	These results indicate that BGN/CH hybrid scaffold developed is a potential candidate for bone tissue engineering.
32588539	12	19	theme	new	1492:1494	arg1	tissue					1496:1501	the new tissue	1488:1501	the new tissue	1488:1501	The in vitro assays showed adequate degradation properties, which is essential for the potential replacement by the new tissue.
32588539	3	20	theme	composite	358:366	arg1	materials					368:376	biodegradable polymer/bioactive glass composite materials	320:376	biodegradable polymer/bioactive glass composite materials	320:376	A new family of biodegradable polymer/bioactive glass composite materials has been designed to be used in bone regeneration approaches.
32588539	1	21	theme	alternative	160:170	arg1	popularity					143:152	popularity	143:152	popularity	143:152	Bone tissue engineering is gaining popularity as an alternative method for the treatment of osseous defects.
32588539	1	21	theme	alternative	160:170	arg1	method					172:177	an alternative method	157:177	an alternative method for the treatment of osseous defects	157:214	Bone tissue engineering is gaining popularity as an alternative method for the treatment of osseous defects.
32588539	3	22	theme	polymer/bioactive	334:350	arg1	materials					368:376	biodegradable polymer/bioactive glass composite materials	320:376	biodegradable polymer/bioactive glass composite materials	320:376	A new family of biodegradable polymer/bioactive glass composite materials has been designed to be used in bone regeneration approaches.
32588539	4	23	theme	bioactive	493:501	arg1	BGN					524:526	BGN	524:526	BGN	524:526	In this work, a hybrid scaffold of chitosan (CH) and bioactive glass nanoparticles (BGN) was prepared by the freeze-gelation method.
32588539	4	23	theme	bioactive	493:501	arg1	nanoparticles					509:521	bioactive glass nanoparticles	493:521	bioactive glass nanoparticles (BGN)	493:527	In this work, a hybrid scaffold of chitosan (CH) and bioactive glass nanoparticles (BGN) was prepared by the freeze-gelation method.
32588539	0	24	theme	engineering	82:92	arg1	applications					94:105	tissue engineering applications	75:105	tissue engineering applications	75:105	Development of hybrid scaffold: Bioactive glass nanoparticles/chitosan for tissue engineering applications.
32588539	7	25	theme	surface	908:914	arg1	area					916:919	the increased surface area	894:919	the increased surface area	894:919	Brunauer-Emmett-Teller results showed the increased surface area and porosity volume of our composite with decreasing BGN proportion.
32588539	14	26	theme	potential	1741:1749	arg1	candidate					1751:1759	a potential candidate	1739:1759	a potential candidate for bone tissue engineering	1739:1787	These results indicate that BGN/CH hybrid scaffold developed is a potential candidate for bone tissue engineering.
32588539	14	26	theme	potential	1741:1749	arg1	scaffold					1717:1724	BGN/CH hybrid scaffold	1703:1724	BGN/CH hybrid scaffold developed	1703:1734	These results indicate that BGN/CH hybrid scaffold developed is a potential candidate for bone tissue engineering.
32588539	12	27	theme	potential	1463:1471	arg1	replacement					1473:1483	the potential replacement	1459:1483	the potential replacement by the new tissue	1459:1501	The in vitro assays showed adequate degradation properties, which is essential for the potential replacement by the new tissue.
32588539	4	28	theme	nanoparticles	509:521	arg1	scaffold					463:470	a hybrid scaffold	454:470	a hybrid scaffold of chitosan (CH) and bioactive glass nanoparticles (BGN)	454:527	In this work, a hybrid scaffold of chitosan (CH) and bioactive glass nanoparticles (BGN) was prepared by the freeze-gelation method.
32588539	13	29	theme	hybrid	1603:1608	arg1	scaffold					1610:1617	the hybrid scaffold	1599:1617	the hybrid scaffold	1599:1617	The in vitro bioactivity studies confirmed the formation of an apatite layer on the surface of the hybrid scaffold, which results in a direct bone bonding of the implant.
32588539	10	30	theme	X-ray	1227:1231	arg1	diffraction					1233:1243	X-ray diffraction	1227:1243	X-ray diffraction study	1227:1249	X-ray diffraction study confirmed the amorphous state of the BGN/CH hybrid scaffold.
32588539	8	31	theme	hybrid	997:1002	arg1	scaffold					1004:1011	BGN/CH hybrid scaffold	990:1011	BGN/CH hybrid scaffold	990:1011	BGN/CH hybrid scaffold was characterized by using physicochemical techniques.
32588539	1	32	theme	Bone	108:111	arg1	engineering					120:130	Bone tissue engineering	108:130	Bone tissue engineering	108:130	Bone tissue engineering is gaining popularity as an alternative method for the treatment of osseous defects.
32588539	9	33	theme	Obtained	1068:1075	arg1	results					1077:1083	Obtained results	1068:1083	Obtained results	1068:1083	Obtained results showed a macroporous morphology of the scaffold with a pore size of about 200 μm, and a homogeneous distribution of the BGN in the CH matrix.
32588539	0	34	theme	hybrid	15:20	arg1	scaffold					22:29	hybrid scaffold	15:29	hybrid scaffold	15:29	Development of hybrid scaffold: Bioactive glass nanoparticles/chitosan for tissue engineering applications.
32588539	4	35	theme	freeze-gelation	549:563	arg1	method					565:570	the freeze-gelation method	545:570	the freeze-gelation method	545:570	In this work, a hybrid scaffold of chitosan (CH) and bioactive glass nanoparticles (BGN) was prepared by the freeze-gelation method.
32588539	14	36	theme	hybrid	1710:1715	arg1	candidate					1751:1759	a potential candidate	1739:1759	a potential candidate for bone tissue engineering	1739:1787	These results indicate that BGN/CH hybrid scaffold developed is a potential candidate for bone tissue engineering.
32588539	14	36	theme	hybrid	1710:1715	arg1	scaffold					1717:1724	BGN/CH hybrid scaffold	1703:1724	BGN/CH hybrid scaffold developed	1703:1734	These results indicate that BGN/CH hybrid scaffold developed is a potential candidate for bone tissue engineering.
32588539	8	37	theme	physicochemical	1040:1054	arg1	techniques					1056:1065	physicochemical techniques	1040:1065	physicochemical techniques	1040:1065	BGN/CH hybrid scaffold was characterized by using physicochemical techniques.
32588539	3	38	theme	regeneration	415:426	arg1	approaches					428:437	bone regeneration approaches	410:437	bone regeneration approaches	410:437	A new family of biodegradable polymer/bioactive glass composite materials has been designed to be used in bone regeneration approaches.
32588539	9	39	theme	macroporous	1094:1104	arg1	morphology					1106:1115	a macroporous morphology	1092:1115	a macroporous morphology of the scaffold with a pore size of about 200 μm	1092:1164	Obtained results showed a macroporous morphology of the scaffold with a pore size of about 200 μm, and a homogeneous distribution of the BGN in the CH matrix.
32588539	0	40	theme	glass	42:46	arg1	nanoparticles/chitosan					48:69	Bioactive glass nanoparticles/chitosan	32:69	Development of hybrid scaffold: Bioactive glass nanoparticles/chitosan for tissue engineering applications.	0:106	Development of hybrid scaffold: Bioactive glass nanoparticles/chitosan for tissue engineering applications.
32588539	6	41	theme	hybrid	817:822	arg1	%					852:852	20, 40, 60, and 80%	834:852	20, 40, 60, and 80%	834:852	In this work, several BGN/CH composites have been prepared by varying the proportion of BGN in the hybrid scaffold (20, 40, 60, and 80%).
32588539	6	41	theme	hybrid	817:822	arg1	scaffold					824:831	the hybrid scaffold	813:831	the hybrid scaffold (20, 40, 60, and 80%)	813:853	In this work, several BGN/CH composites have been prepared by varying the proportion of BGN in the hybrid scaffold (20, 40, 60, and 80%).
32588539	13	42	theme	layer	1575:1579	arg1	formation					1551:1559	the formation	1547:1559	the formation of an apatite layer on the surface of the hybrid scaffold, which results in a direct bone bonding of the implant	1547:1672	The in vitro bioactivity studies confirmed the formation of an apatite layer on the surface of the hybrid scaffold, which results in a direct bone bonding of the implant.
32588539	13	43	from	layer	1575:1579	arg1	surface					1588:1594	the surface	1584:1594	the surface of the hybrid scaffold	1584:1617	The in vitro bioactivity studies confirmed the formation of an apatite layer on the surface of the hybrid scaffold, which results in a direct bone bonding of the implant.
32588539	9	44	theme	scaffold	1124:1131	arg1	morphology					1106:1115	a macroporous morphology	1092:1115	a macroporous morphology of the scaffold with a pore size of about 200 μm	1092:1164	Obtained results showed a macroporous morphology of the scaffold with a pore size of about 200 μm, and a homogeneous distribution of the BGN in the CH matrix.
32588539	9	44	theme	scaffold	1124:1131	arg1	distribution					1185:1196	a homogeneous distribution	1171:1196	a homogeneous distribution of the BGN in the CH matrix	1171:1224	Obtained results showed a macroporous morphology of the scaffold with a pore size of about 200 μm, and a homogeneous distribution of the BGN in the CH matrix.
32588539	0	45	dep	Development	0:10	arg1	nanoparticles/chitosan					48:69	Bioactive glass nanoparticles/chitosan	32:69	Development of hybrid scaffold: Bioactive glass nanoparticles/chitosan for tissue engineering applications.	0:106	Development of hybrid scaffold: Bioactive glass nanoparticles/chitosan for tissue engineering applications.
32588539	13	46	from	formation	1551:1559	arg1	surface					1588:1594	the surface	1584:1594	the surface of the hybrid scaffold	1584:1617	The in vitro bioactivity studies confirmed the formation of an apatite layer on the surface of the hybrid scaffold, which results in a direct bone bonding of the implant.
32588539	5	47	theme	acid	643:646	arg1	concentration					619:631	the concentration	615:631	the concentration of acetic acid	615:646	This method has been studied by adjusting the concentration of acetic acid; this process can influence the structure properties of the scaffold.
32588539	10	48	theme	hybrid	1295:1300	arg1	scaffold					1302:1309	the BGN/CH hybrid scaffold	1284:1309	the BGN/CH hybrid scaffold	1284:1309	X-ray diffraction study confirmed the amorphous state of the BGN/CH hybrid scaffold.
32588539	12	49	theme	adequate	1403:1410	arg1	properties					1424:1433	adequate degradation properties	1403:1433	adequate degradation properties	1403:1433	The in vitro assays showed adequate degradation properties, which is essential for the potential replacement by the new tissue.
32588539	6	50	theme	BGN/CH	740:745	arg1	composites					747:756	several BGN/CH composites	732:756	several BGN/CH composites	732:756	In this work, several BGN/CH composites have been prepared by varying the proportion of BGN in the hybrid scaffold (20, 40, 60, and 80%).
32588539	9	51	from	distribution	1185:1196	arg1	matrix					1219:1224	the CH matrix	1212:1224	the CH matrix	1212:1224	Obtained results showed a macroporous morphology of the scaffold with a pore size of about 200 μm, and a homogeneous distribution of the BGN in the CH matrix.
32588539	12	52	theme	in	1380:1381	arg1	assays					1389:1394	The in vitro assays	1376:1394	The in vitro assays	1376:1394	The in vitro assays showed adequate degradation properties, which is essential for the potential replacement by the new tissue.
32588539	13	53	theme	implant	1666:1672	arg1	bonding					1651:1657	a direct bone bonding	1637:1657	a direct bone bonding of the implant	1637:1672	The in vitro bioactivity studies confirmed the formation of an apatite layer on the surface of the hybrid scaffold, which results in a direct bone bonding of the implant.
32588539	13	54	theme	in	1508:1509	arg1	studies					1529:1535	The in vitro bioactivity studies	1504:1535	The in vitro bioactivity studies	1504:1535	The in vitro bioactivity studies confirmed the formation of an apatite layer on the surface of the hybrid scaffold, which results in a direct bone bonding of the implant.
32588539	13	55	theme	bioactivity	1517:1527	arg1	studies					1529:1535	The in vitro bioactivity studies	1504:1535	The in vitro bioactivity studies	1504:1535	The in vitro bioactivity studies confirmed the formation of an apatite layer on the surface of the hybrid scaffold, which results in a direct bone bonding of the implant.
32588539	1	56	theme	defects	208:214	arg1	treatment					187:195	the treatment	183:195	the treatment of osseous defects	183:214	Bone tissue engineering is gaining popularity as an alternative method for the treatment of osseous defects.
32588539	13	57	theme	bone	1646:1649	arg1	bonding					1651:1657	a direct bone bonding	1637:1657	a direct bone bonding of the implant	1637:1672	The in vitro bioactivity studies confirmed the formation of an apatite layer on the surface of the hybrid scaffold, which results in a direct bone bonding of the implant.
32588539	4	58	theme	chitosan	475:482	arg1	scaffold					463:470	a hybrid scaffold	454:470	a hybrid scaffold of chitosan (CH) and bioactive glass nanoparticles (BGN)	454:527	In this work, a hybrid scaffold of chitosan (CH) and bioactive glass nanoparticles (BGN) was prepared by the freeze-gelation method.
32588539	2	59	theme	biodegradable	229:241	arg1	polymers					243:250	biodegradable polymers	229:250	biodegradable polymers	229:250	A number of biodegradable polymers have been explored for tissue engineering purposes.
32588539	3	60	theme	biodegradable	320:332	arg1	materials					368:376	biodegradable polymer/bioactive glass composite materials	320:376	biodegradable polymer/bioactive glass composite materials	320:376	A new family of biodegradable polymer/bioactive glass composite materials has been designed to be used in bone regeneration approaches.
32588539	7	61	theme	BGN	974:976	arg1	proportion					978:987	BGN proportion	974:987	BGN proportion	974:987	Brunauer-Emmett-Teller results showed the increased surface area and porosity volume of our composite with decreasing BGN proportion.
32588539	2	62	theme	tissue	275:280	arg1	purposes					294:301	tissue engineering purposes	275:301	tissue engineering purposes	275:301	A number of biodegradable polymers have been explored for tissue engineering purposes.
32588539	7	63	theme	composite	948:956	arg1	volume					934:939	porosity volume	925:939	porosity volume	925:939	Brunauer-Emmett-Teller results showed the increased surface area and porosity volume of our composite with decreasing BGN proportion.
32588539	7	63	theme	composite	948:956	arg1	area					916:919	the increased surface area	894:919	the increased surface area	894:919	Brunauer-Emmett-Teller results showed the increased surface area and porosity volume of our composite with decreasing BGN proportion.
32588539	0	64	theme	tissue	75:80	arg1	applications					94:105	tissue engineering applications	75:105	tissue engineering applications	75:105	Development of hybrid scaffold: Bioactive glass nanoparticles/chitosan for tissue engineering applications.
32588539	9	65	theme	CH	1216:1217	arg1	matrix					1219:1224	the CH matrix	1212:1224	the CH matrix	1212:1224	Obtained results showed a macroporous morphology of the scaffold with a pore size of about 200 μm, and a homogeneous distribution of the BGN in the CH matrix.
32588539	14	66	theme	bone	1765:1768	arg1	engineering					1777:1787	bone tissue engineering	1765:1787	bone tissue engineering	1765:1787	These results indicate that BGN/CH hybrid scaffold developed is a potential candidate for bone tissue engineering.
32588539	3	67	theme	materials	368:376	arg1	family					310:315	A new family	304:315	A new family of biodegradable polymer/bioactive glass composite materials	304:376	A new family of biodegradable polymer/bioactive glass composite materials has been designed to be used in bone regeneration approaches.
32588539	4	68	theme	glass	503:507	arg1	BGN					524:526	BGN	524:526	BGN	524:526	In this work, a hybrid scaffold of chitosan (CH) and bioactive glass nanoparticles (BGN) was prepared by the freeze-gelation method.
32588539	4	68	theme	glass	503:507	arg1	nanoparticles					509:521	bioactive glass nanoparticles	493:521	bioactive glass nanoparticles (BGN)	493:527	In this work, a hybrid scaffold of chitosan (CH) and bioactive glass nanoparticles (BGN) was prepared by the freeze-gelation method.
32588539	7	69	theme	porosity	925:932	arg1	volume					934:939	porosity volume	925:939	porosity volume	925:939	Brunauer-Emmett-Teller results showed the increased surface area and porosity volume of our composite with decreasing BGN proportion.
32588539	3	70	theme	glass	352:356	arg1	materials					368:376	biodegradable polymer/bioactive glass composite materials	320:376	biodegradable polymer/bioactive glass composite materials	320:376	A new family of biodegradable polymer/bioactive glass composite materials has been designed to be used in bone regeneration approaches.
32588539	11	71	from	Interaction	1312:1322	arg1	composite					1351:1359	the composite	1347:1359	the composite	1347:1359	Interaction between CH and BGNs in the composite was confirmed.
32588539	8	72	theme	BGN/CH	990:995	arg1	scaffold					1004:1011	BGN/CH hybrid scaffold	990:1011	BGN/CH hybrid scaffold	990:1011	BGN/CH hybrid scaffold was characterized by using physicochemical techniques.
32588539	13	73	theme	direct	1639:1644	arg1	bonding					1651:1657	a direct bone bonding	1637:1657	a direct bone bonding of the implant	1637:1672	The in vitro bioactivity studies confirmed the formation of an apatite layer on the surface of the hybrid scaffold, which results in a direct bone bonding of the implant.
32588539	10	74	theme	diffraction	1233:1243	arg1	study					1245:1249	X-ray diffraction study	1227:1249	X-ray diffraction study	1227:1249	X-ray diffraction study confirmed the amorphous state of the BGN/CH hybrid scaffold.
32588539	7	75	theme	increased	898:906	arg1	area					916:919	the increased surface area	894:919	the increased surface area	894:919	Brunauer-Emmett-Teller results showed the increased surface area and porosity volume of our composite with decreasing BGN proportion.
32588539	13	76	theme	scaffold	1610:1617	arg1	surface					1588:1594	the surface	1584:1594	the surface of the hybrid scaffold	1584:1617	The in vitro bioactivity studies confirmed the formation of an apatite layer on the surface of the hybrid scaffold, which results in a direct bone bonding of the implant.
32588539	3	77	theme	bone	410:413	arg1	approaches					428:437	bone regeneration approaches	410:437	bone regeneration approaches	410:437	A new family of biodegradable polymer/bioactive glass composite materials has been designed to be used in bone regeneration approaches.
32588539	10	78	theme	amorphous	1265:1273	arg1	state					1275:1279	the amorphous state	1261:1279	the amorphous state of the BGN/CH hybrid scaffold	1261:1309	X-ray diffraction study confirmed the amorphous state of the BGN/CH hybrid scaffold.
32588539	0	79	theme	scaffold	22:29	arg1	Development					0:10	Development	0:10	Development of hybrid scaffold: Bioactive glass nanoparticles/chitosan for tissue engineering applications.	0:106	Development of hybrid scaffold: Bioactive glass nanoparticles/chitosan for tissue engineering applications.
32588539	3	80	used	used	402:405	arg2	family					310:315	A new family	304:315	A new family of biodegradable polymer/bioactive glass composite materials	304:376	A new family of biodegradable polymer/bioactive glass composite materials has been designed to be used in bone regeneration approaches.
32588539	7	81	theme	Brunauer-Emmett-Teller	856:877	arg1	results					879:885	Brunauer-Emmett-Teller results	856:885	Brunauer-Emmett-Teller results	856:885	Brunauer-Emmett-Teller results showed the increased surface area and porosity volume of our composite with decreasing BGN proportion.
32588539	1	82	theme	tissue	113:118	arg1	engineering					120:130	Bone tissue engineering	108:130	Bone tissue engineering	108:130	Bone tissue engineering is gaining popularity as an alternative method for the treatment of osseous defects.
32588539	0	83	theme	Bioactive	32:40	arg1	nanoparticles/chitosan					48:69	Bioactive glass nanoparticles/chitosan	32:69	Development of hybrid scaffold: Bioactive glass nanoparticles/chitosan for tissue engineering applications.	0:106	Development of hybrid scaffold: Bioactive glass nanoparticles/chitosan for tissue engineering applications.
32588539	10	84	theme	BGN/CH	1288:1293	arg1	scaffold					1302:1309	the BGN/CH hybrid scaffold	1284:1309	the BGN/CH hybrid scaffold	1284:1309	X-ray diffraction study confirmed the amorphous state of the BGN/CH hybrid scaffold.
32588539	14	85	theme	BGN/CH	1703:1708	arg1	candidate					1751:1759	a potential candidate	1739:1759	a potential candidate for bone tissue engineering	1739:1787	These results indicate that BGN/CH hybrid scaffold developed is a potential candidate for bone tissue engineering.
32588539	14	85	theme	BGN/CH	1703:1708	arg1	scaffold					1717:1724	BGN/CH hybrid scaffold	1703:1724	BGN/CH hybrid scaffold developed	1703:1734	These results indicate that BGN/CH hybrid scaffold developed is a potential candidate for bone tissue engineering.
32588539	13	86	theme	apatite	1567:1573	arg1	layer					1575:1579	an apatite layer	1564:1579	an apatite layer	1564:1579	The in vitro bioactivity studies confirmed the formation of an apatite layer on the surface of the hybrid scaffold, which results in a direct bone bonding of the implant.
32588539	6	87	theme	several	732:738	arg1	composites					747:756	several BGN/CH composites	732:756	several BGN/CH composites	732:756	In this work, several BGN/CH composites have been prepared by varying the proportion of BGN in the hybrid scaffold (20, 40, 60, and 80%).
32588539	12	88	theme	degradation	1412:1422	arg1	properties					1424:1433	adequate degradation properties	1403:1433	adequate degradation properties	1403:1433	The in vitro assays showed adequate degradation properties, which is essential for the potential replacement by the new tissue.
32588539	6	89	theme	BGN	806:808	arg1	proportion					792:801	the proportion	788:801	the proportion of BGN	788:808	In this work, several BGN/CH composites have been prepared by varying the proportion of BGN in the hybrid scaffold (20, 40, 60, and 80%).
32588539	10	90	theme	scaffold	1302:1309	arg1	state					1275:1279	the amorphous state	1261:1279	the amorphous state of the BGN/CH hybrid scaffold	1261:1309	X-ray diffraction study confirmed the amorphous state of the BGN/CH hybrid scaffold.
32588539	9	91	with	scaffold	1124:1131	arg1	size					1145:1148	a pore size	1138:1148	a pore size of about 200 μm	1138:1164	Obtained results showed a macroporous morphology of the scaffold with a pore size of about 200 μm, and a homogeneous distribution of the BGN in the CH matrix.
33736273	0	0	theme	sensitive	95:103	arg1	microscopy					120:129	chemically sensitive scanning probe microscopy	84:129	chemically sensitive scanning probe microscopy	84:129	Differences in surface chemistry of regenerated lignocellulose fibers determined by chemically sensitive scanning probe microscopy.
33736273	8	1	from	gradients	1218:1226	arg1	types					1246:1250	specific fiber types	1231:1250	specific fiber types	1231:1250	With this setup, infrared signals with a spatial resolution below 20 nm reveal chemical gradients in specific fiber types.
33736273	5	2	theme	atomic	782:787	arg1	microscopy					795:804	atomic force microscopy	782:804	hydrophilic and hydrophobic atomic force microscopy tips	754:809	Adhesion properties were visualized with chemical force microscopy and showed contrasts towards hydrophilic and hydrophobic atomic force microscopy tips.
33736273	1	3	theme	fibers	186:191	arg1	Tuning					132:137	Tuning the composition	132:153	Tuning the composition of regenerated lignocellulosic fibers in the production process	132:217	Tuning the composition of regenerated lignocellulosic fibers in the production process enables targeting of specific material properties.
33736273	8	4	with	signals	1156:1162	arg1	resolution					1179:1188	a spatial resolution	1169:1188	a spatial resolution below 20 nm	1169:1200	With this setup, infrared signals with a spatial resolution below 20 nm reveal chemical gradients in specific fiber types.
33736273	5	5	theme	force	789:793	arg1	microscopy					795:804	atomic force microscopy	782:804	hydrophilic and hydrophobic atomic force microscopy tips	754:809	Adhesion properties were visualized with chemical force microscopy and showed contrasts towards hydrophilic and hydrophobic atomic force microscopy tips.
33736273	2	6	theme	fibers	410:415	arg1	components					384:393	chemical components	375:393	chemical components of regenerated fibers	375:415	In composite materials, such properties could be manipulated by controlled heterogeneous distribution of chemical components of regenerated fibers.
33736273	7	7	with	spectroscopic	982:994	arg1	resolution					1021:1030	nanometer resolution	1011:1030	nanometer resolution	1011:1030	Additionally, peak force infrared microscopy mapped spectroscopic contrasts with nanometer resolution and provided point infrared spectra, which were consistent to classical infrared microscopy data.
33736273	0	8	theme	probe	114:118	arg1	microscopy					120:129	chemically sensitive scanning probe microscopy	84:129	chemically sensitive scanning probe microscopy	84:129	Differences in surface chemistry of regenerated lignocellulose fibers determined by chemically sensitive scanning probe microscopy.
33736273	0	9	from	Differences	0:10	arg1	chemistry					23:31	surface chemistry	15:31	surface chemistry of regenerated lignocellulose fibers	15:68	Differences in surface chemistry of regenerated lignocellulose fibers determined by chemically sensitive scanning probe microscopy.
33736273	5	10	theme	microscopy	795:804	arg1	tips					806:809	hydrophilic and hydrophobic atomic force microscopy tips	754:809	hydrophilic and hydrophobic atomic force microscopy tips	754:809	Adhesion properties were visualized with chemical force microscopy and showed contrasts towards hydrophilic and hydrophobic atomic force microscopy tips.
33736273	7	11	theme	microscopy	1113:1122	arg1	data					1124:1127	classical infrared microscopy data	1094:1127	classical infrared microscopy data	1094:1127	Additionally, peak force infrared microscopy mapped spectroscopic contrasts with nanometer resolution and provided point infrared spectra, which were consistent to classical infrared microscopy data.
33736273	0	12	theme	scanning	105:112	arg1	microscopy					120:129	chemically sensitive scanning probe microscopy	84:129	chemically sensitive scanning probe microscopy	84:129	Differences in surface chemistry of regenerated lignocellulose fibers determined by chemically sensitive scanning probe microscopy.
33736273	8	13	theme	spatial	1171:1177	arg1	resolution					1179:1188	a spatial resolution	1169:1188	a spatial resolution below 20 nm	1169:1200	With this setup, infrared signals with a spatial resolution below 20 nm reveal chemical gradients in specific fiber types.
33736273	7	14	theme	point	1045:1049	arg1	spectra					1060:1066	point infrared spectra	1045:1066	point infrared spectra	1045:1066	Additionally, peak force infrared microscopy mapped spectroscopic contrasts with nanometer resolution and provided point infrared spectra, which were consistent to classical infrared microscopy data.
33736273	1	15	dep	Tuning	132:137	arg1	composition					143:153	the composition	139:153	Tuning the composition of regenerated lignocellulosic fibers in the production process	132:217	Tuning the composition of regenerated lignocellulosic fibers in the production process enables targeting of specific material properties.
33736273	6	16	theme	adhesion	857:864	arg1	properties					866:875	heterogeneous adhesion properties	843:875	heterogeneous adhesion properties	843:875	Fibers containing xylan showed heterogeneous adhesion properties within the fiber structure towards hydrophilic tips.
33736273	4	17	theme	surface	578:584	arg1	chemistry					586:594	the surface chemistry	574:594	the surface chemistry of four differently tuned regenerated lignocellulosic fibers	574:655	We compared complementary microscopic techniques to analyze the surface chemistry of four differently tuned regenerated lignocellulosic fibers.
33736273	5	18	theme	hydrophilic	754:764	arg1	tips					806:809	hydrophilic and hydrophobic atomic force microscopy tips	754:809	hydrophilic and hydrophobic atomic force microscopy tips	754:809	Adhesion properties were visualized with chemical force microscopy and showed contrasts towards hydrophilic and hydrophobic atomic force microscopy tips.
33736273	6	19	theme	heterogeneous	843:855	arg1	properties					866:875	heterogeneous adhesion properties	843:875	heterogeneous adhesion properties	843:875	Fibers containing xylan showed heterogeneous adhesion properties within the fiber structure towards hydrophilic tips.
33736273	1	20	theme	production	200:209	arg1	process					211:217	the production process	196:217	the production process	196:217	Tuning the composition of regenerated lignocellulosic fibers in the production process enables targeting of specific material properties.
33736273	3	21	theme	visualization	444:456	arg1	method					458:463	a visualization method	442:463	a visualization method to show their inherent chemical characteristics	442:511	This attribute requires a visualization method to show their inherent chemical characteristics.
33736273	6	22	contain	containing	819:828	arg2	xylan					830:834	xylan	830:834	xylan	830:834	Fibers containing xylan showed heterogeneous adhesion properties within the fiber structure towards hydrophilic tips.
33736273	6	22	contain	containing	819:828	arg1	Fibers					812:817	Fibers	812:817	Fibers containing xylan	812:834	Fibers containing xylan showed heterogeneous adhesion properties within the fiber structure towards hydrophilic tips.
33736273	3	23	theme	chemical	488:495	arg1	characteristics					497:511	their inherent chemical characteristics	473:511	their inherent chemical characteristics	473:511	This attribute requires a visualization method to show their inherent chemical characteristics.
33736273	5	24	theme	hydrophobic	770:780	arg1	tips					806:809	hydrophilic and hydrophobic atomic force microscopy tips	754:809	hydrophilic and hydrophobic atomic force microscopy tips	754:809	Adhesion properties were visualized with chemical force microscopy and showed contrasts towards hydrophilic and hydrophobic atomic force microscopy tips.
33736273	1	25	from	Tuning	132:137	arg1	process					211:217	the production process	196:217	the production process	196:217	Tuning the composition of regenerated lignocellulosic fibers in the production process enables targeting of specific material properties.
33736273	7	26	theme	nanometer	1011:1019	arg1	resolution					1021:1030	nanometer resolution	1011:1030	nanometer resolution	1011:1030	Additionally, peak force infrared microscopy mapped spectroscopic contrasts with nanometer resolution and provided point infrared spectra, which were consistent to classical infrared microscopy data.
33736273	8	27	theme	chemical	1209:1216	arg1	gradients					1218:1226	chemical gradients	1209:1226	chemical gradients in specific fiber types	1209:1250	With this setup, infrared signals with a spatial resolution below 20 nm reveal chemical gradients in specific fiber types.
33736273	5	28	theme	chemical	699:706	arg1	microscopy					714:723	chemical force microscopy	699:723	chemical force microscopy	699:723	Adhesion properties were visualized with chemical force microscopy and showed contrasts towards hydrophilic and hydrophobic atomic force microscopy tips.
33736273	5	29	theme	force	708:712	arg1	microscopy					714:723	chemical force microscopy	699:723	chemical force microscopy	699:723	Adhesion properties were visualized with chemical force microscopy and showed contrasts towards hydrophilic and hydrophobic atomic force microscopy tips.
33736273	2	30	theme	heterogeneous	345:357	arg1	distribution					359:370	controlled heterogeneous distribution	334:370	controlled heterogeneous distribution of chemical components of regenerated fibers	334:415	In composite materials, such properties could be manipulated by controlled heterogeneous distribution of chemical components of regenerated fibers.
33736273	0	31	theme	surface	15:21	arg1	chemistry					23:31	surface chemistry	15:31	surface chemistry of regenerated lignocellulose fibers	15:68	Differences in surface chemistry of regenerated lignocellulose fibers determined by chemically sensitive scanning probe microscopy.
33736273	7	32	theme	infrared	955:962	arg1	microscopy					964:973	peak force infrared microscopy	944:973	peak force infrared microscopy	944:973	Additionally, peak force infrared microscopy mapped spectroscopic contrasts with nanometer resolution and provided point infrared spectra, which were consistent to classical infrared microscopy data.
33736273	4	33	theme	complementary	526:538	arg1	techniques					552:561	complementary microscopic techniques	526:561	complementary microscopic techniques	526:561	We compared complementary microscopic techniques to analyze the surface chemistry of four differently tuned regenerated lignocellulosic fibers.
33736273	2	34	theme	controlled	334:343	arg1	distribution					359:370	controlled heterogeneous distribution	334:370	controlled heterogeneous distribution of chemical components of regenerated fibers	334:415	In composite materials, such properties could be manipulated by controlled heterogeneous distribution of chemical components of regenerated fibers.
33736273	0	35	theme	regenerated	36:46	arg1	fibers					63:68	regenerated lignocellulose fibers	36:68	regenerated lignocellulose fibers	36:68	Differences in surface chemistry of regenerated lignocellulose fibers determined by chemically sensitive scanning probe microscopy.
33736273	7	36	theme	infrared	1051:1058	arg1	spectra					1060:1066	point infrared spectra	1045:1066	point infrared spectra	1045:1066	Additionally, peak force infrared microscopy mapped spectroscopic contrasts with nanometer resolution and provided point infrared spectra, which were consistent to classical infrared microscopy data.
33736273	7	37	dep	spectroscopic	982:994	arg1	contrasts					996:1004	contrasts	996:1004	contrasts	996:1004	Additionally, peak force infrared microscopy mapped spectroscopic contrasts with nanometer resolution and provided point infrared spectra, which were consistent to classical infrared microscopy data.
33736273	2	38	theme	composite	273:281	arg1	materials					283:291	composite materials	273:291	composite materials	273:291	In composite materials, such properties could be manipulated by controlled heterogeneous distribution of chemical components of regenerated fibers.
33736273	1	39	theme	specific	240:247	arg1	properties					258:267	specific material properties	240:267	specific material properties	240:267	Tuning the composition of regenerated lignocellulosic fibers in the production process enables targeting of specific material properties.
33736273	3	40	theme	inherent	479:486	arg1	characteristics					497:511	their inherent chemical characteristics	473:511	their inherent chemical characteristics	473:511	This attribute requires a visualization method to show their inherent chemical characteristics.
33736273	4	41	theme	regenerated	622:632	arg1	fibers					650:655	four differently tuned regenerated lignocellulosic fibers	599:655	four differently tuned regenerated lignocellulosic fibers	599:655	We compared complementary microscopic techniques to analyze the surface chemistry of four differently tuned regenerated lignocellulosic fibers.
33736273	1	42	theme	material	249:256	arg1	properties					258:267	specific material properties	240:267	specific material properties	240:267	Tuning the composition of regenerated lignocellulosic fibers in the production process enables targeting of specific material properties.
33736273	0	43	theme	fibers	63:68	arg1	chemistry					23:31	surface chemistry	15:31	surface chemistry of regenerated lignocellulose fibers	15:68	Differences in surface chemistry of regenerated lignocellulose fibers determined by chemically sensitive scanning probe microscopy.
33736273	4	44	theme	tuned	616:620	arg1	fibers					650:655	four differently tuned regenerated lignocellulosic fibers	599:655	four differently tuned regenerated lignocellulosic fibers	599:655	We compared complementary microscopic techniques to analyze the surface chemistry of four differently tuned regenerated lignocellulosic fibers.
33736273	4	45	theme	microscopic	540:550	arg1	techniques					552:561	complementary microscopic techniques	526:561	complementary microscopic techniques	526:561	We compared complementary microscopic techniques to analyze the surface chemistry of four differently tuned regenerated lignocellulosic fibers.
33736273	5	46	theme	Adhesion	658:665	arg1	properties					667:676	Adhesion properties	658:676	Adhesion properties	658:676	Adhesion properties were visualized with chemical force microscopy and showed contrasts towards hydrophilic and hydrophobic atomic force microscopy tips.
33736273	2	47	theme	regenerated	398:408	arg1	fibers					410:415	regenerated fibers	398:415	regenerated fibers	398:415	In composite materials, such properties could be manipulated by controlled heterogeneous distribution of chemical components of regenerated fibers.
33736273	0	48	theme	lignocellulose	48:61	arg1	fibers					63:68	regenerated lignocellulose fibers	36:68	regenerated lignocellulose fibers	36:68	Differences in surface chemistry of regenerated lignocellulose fibers determined by chemically sensitive scanning probe microscopy.
33736273	4	49	theme	fibers	650:655	arg1	chemistry					586:594	the surface chemistry	574:594	the surface chemistry of four differently tuned regenerated lignocellulosic fibers	574:655	We compared complementary microscopic techniques to analyze the surface chemistry of four differently tuned regenerated lignocellulosic fibers.
33736273	8	50	theme	fiber	1240:1244	arg1	types					1246:1250	specific fiber types	1231:1250	specific fiber types	1231:1250	With this setup, infrared signals with a spatial resolution below 20 nm reveal chemical gradients in specific fiber types.
33736273	6	51	theme	hydrophilic	912:922	arg1	tips					924:927	hydrophilic tips	912:927	hydrophilic tips	912:927	Fibers containing xylan showed heterogeneous adhesion properties within the fiber structure towards hydrophilic tips.
33736273	8	52	theme	specific	1231:1238	arg1	types					1246:1250	specific fiber types	1231:1250	specific fiber types	1231:1250	With this setup, infrared signals with a spatial resolution below 20 nm reveal chemical gradients in specific fiber types.
33736273	7	53	theme	classical	1094:1102	arg1	data					1124:1127	classical infrared microscopy data	1094:1127	classical infrared microscopy data	1094:1127	Additionally, peak force infrared microscopy mapped spectroscopic contrasts with nanometer resolution and provided point infrared spectra, which were consistent to classical infrared microscopy data.
33736273	4	54	theme	lignocellulosic	634:648	arg1	fibers					650:655	four differently tuned regenerated lignocellulosic fibers	599:655	four differently tuned regenerated lignocellulosic fibers	599:655	We compared complementary microscopic techniques to analyze the surface chemistry of four differently tuned regenerated lignocellulosic fibers.
33736273	2	55	theme	components	384:393	arg1	distribution					359:370	controlled heterogeneous distribution	334:370	controlled heterogeneous distribution of chemical components of regenerated fibers	334:415	In composite materials, such properties could be manipulated by controlled heterogeneous distribution of chemical components of regenerated fibers.
33736273	7	56	theme	infrared	1104:1111	arg1	data					1124:1127	classical infrared microscopy data	1094:1127	classical infrared microscopy data	1094:1127	Additionally, peak force infrared microscopy mapped spectroscopic contrasts with nanometer resolution and provided point infrared spectra, which were consistent to classical infrared microscopy data.
33736273	1	57	theme	properties	258:267	arg1	targeting					227:235	targeting	227:235	targeting of specific material properties	227:267	Tuning the composition of regenerated lignocellulosic fibers in the production process enables targeting of specific material properties.
33736273	2	58	theme	such	294:297	arg1	properties					299:308	such properties	294:308	such properties	294:308	In composite materials, such properties could be manipulated by controlled heterogeneous distribution of chemical components of regenerated fibers.
33736273	2	59	theme	chemical	375:382	arg1	components					384:393	chemical components	375:393	chemical components of regenerated fibers	375:415	In composite materials, such properties could be manipulated by controlled heterogeneous distribution of chemical components of regenerated fibers.
33736273	7	60	theme	peak	944:947	arg1	microscopy					964:973	peak force infrared microscopy	944:973	peak force infrared microscopy	944:973	Additionally, peak force infrared microscopy mapped spectroscopic contrasts with nanometer resolution and provided point infrared spectra, which were consistent to classical infrared microscopy data.
33736273	6	61	theme	fiber	888:892	arg1	structure					894:902	the fiber structure	884:902	the fiber structure towards hydrophilic tips	884:927	Fibers containing xylan showed heterogeneous adhesion properties within the fiber structure towards hydrophilic tips.
33736273	1	62	theme	regenerated	158:168	arg1	fibers					186:191	regenerated lignocellulosic fibers	158:191	regenerated lignocellulosic fibers	158:191	Tuning the composition of regenerated lignocellulosic fibers in the production process enables targeting of specific material properties.
33736273	8	63	theme	infrared	1147:1154	arg1	signals					1156:1162	infrared signals	1147:1162	infrared signals with a spatial resolution below 20 nm	1147:1200	With this setup, infrared signals with a spatial resolution below 20 nm reveal chemical gradients in specific fiber types.
33736273	1	64	theme	lignocellulosic	170:184	arg1	fibers					186:191	regenerated lignocellulosic fibers	158:191	regenerated lignocellulosic fibers	158:191	Tuning the composition of regenerated lignocellulosic fibers in the production process enables targeting of specific material properties.
33736273	7	65	theme	force	949:953	arg1	microscopy					964:973	peak force infrared microscopy	944:973	peak force infrared microscopy	944:973	Additionally, peak force infrared microscopy mapped spectroscopic contrasts with nanometer resolution and provided point infrared spectra, which were consistent to classical infrared microscopy data.
34582904	8	0	theme	thermal	1184:1190	arg1	stability					1192:1200	thermal stability	1184:1200	thermal stability	1184:1200	TPS/CNF green nanocomposites containing 3 wt% CNFs had exceptional tensile strength (~161%), tensile modulus (~167%), thermal stability, and crystallinity.
34582904	4	1	theme	casting	684:690	arg1	process					692:698	the solution casting process	671:698	the solution casting process	671:698	Following that, green nanocomposite films were made from potato starch using the solution casting process, by which we used glycerol (30 wt%) to make thermoplastic starch.
34582904	9	2	theme	starch	1263:1268	arg1	%					1300:1300	3 wt%	1296:1300	3 wt%	1296:1300	As a result, nanocomposite films made of starch and cellulose nanofibers (3 wt%) extracted from Chrysopogon zizanioides roots would be alternatives for sustainable packaging.
34582904	9	2	theme	starch	1263:1268	arg1	nanofibers					1284:1293	starch and cellulose nanofibers	1263:1293	starch and cellulose nanofibers (3 wt%) extracted from Chrysopogon zizanioides roots	1263:1346	As a result, nanocomposite films made of starch and cellulose nanofibers (3 wt%) extracted from Chrysopogon zizanioides roots would be alternatives for sustainable packaging.
34582904	8	3	theme	3 wt	1106:1109	arg1	CNFs					1112:1115	3 wt% CNFs	1106:1115	3 wt% CNFs	1106:1115	TPS/CNF green nanocomposites containing 3 wt% CNFs had exceptional tensile strength (~161%), tensile modulus (~167%), thermal stability, and crystallinity.
34582904	4	4	theme	solution	675:682	arg1	process					692:698	the solution casting process	671:698	the solution casting process	671:698	Following that, green nanocomposite films were made from potato starch using the solution casting process, by which we used glycerol (30 wt%) to make thermoplastic starch.
34582904	3	5	dep	hydrolysis	548:557	arg1	iii					533:535	iii	533:535	iii	533:535	CNFs were separated using an environmentally friendly acid isolation technique that included three stages: (i) alkali treatment; (ii) bleaching; and (iii) mild acid hydrolysis using oxalic acid in an autoclave.
34582904	3	6	theme	friendly	428:435	arg1	technique					452:460	an environmentally friendly acid isolation technique	409:460	an environmentally friendly acid isolation technique that included three stages: (i) alkali treatment; (ii) bleaching; and (iii) mild acid hydrolysis using oxalic acid in an autoclave	409:591	CNFs were separated using an environmentally friendly acid isolation technique that included three stages: (i) alkali treatment; (ii) bleaching; and (iii) mild acid hydrolysis using oxalic acid in an autoclave.
34582904	3	7	dep	treatment	501:509	arg1	i					491:491	i	491:491	i	491:491	CNFs were separated using an environmentally friendly acid isolation technique that included three stages: (i) alkali treatment; (ii) bleaching; and (iii) mild acid hydrolysis using oxalic acid in an autoclave.
34582904	4	8	used	used	713:716	arg2	we					710:711	we	710:711	we	710:711	Following that, green nanocomposite films were made from potato starch using the solution casting process, by which we used glycerol (30 wt%) to make thermoplastic starch.
34582904	9	9	theme	cellulose	1274:1282	arg1	%					1300:1300	3 wt%	1296:1300	3 wt%	1296:1300	As a result, nanocomposite films made of starch and cellulose nanofibers (3 wt%) extracted from Chrysopogon zizanioides roots would be alternatives for sustainable packaging.
34582904	9	9	theme	cellulose	1274:1282	arg1	nanofibers					1284:1293	starch and cellulose nanofibers	1263:1293	starch and cellulose nanofibers (3 wt%) extracted from Chrysopogon zizanioides roots	1263:1346	As a result, nanocomposite films made of starch and cellulose nanofibers (3 wt%) extracted from Chrysopogon zizanioides roots would be alternatives for sustainable packaging.
34582904	10	10	theme	zizanioides	1434:1444	arg1	roots					1446:1450	Chrysopogon zizanioides roots	1422:1450	Chrysopogon zizanioides roots	1422:1450	It can be concluded that Chrysopogon zizanioides roots have high potential for polymer industry.
34582904	3	11	theme	mild	538:541	arg1	hydrolysis					548:557	(iii) mild acid hydrolysis	532:557	(iii) mild acid hydrolysis	532:557	CNFs were separated using an environmentally friendly acid isolation technique that included three stages: (i) alkali treatment; (ii) bleaching; and (iii) mild acid hydrolysis using oxalic acid in an autoclave.
34582904	1	12	theme	Green	93:97	arg1	films					140:144	Green thermoplastic starch (TPS) nanocomposite films	93:144	Green thermoplastic starch (TPS) nanocomposite films aided by cellulose nanofibers (CNFs) from Chrysopogon zizanioides roots	93:216	Green thermoplastic starch (TPS) nanocomposite films aided by cellulose nanofibers (CNFs) from Chrysopogon zizanioides roots were developed and characterized.
34582904	6	13	theme	isolated	892:899	arg1	CNFs					901:904	The isolated CNFs	888:904	The isolated CNFs	888:904	The isolated CNFs had diameters in the range of 17-27 nm.
34582904	8	14	theme	TPS/CNF	1066:1072	arg1	nanocomposites					1080:1093	TPS/CNF green nanocomposites	1066:1093	TPS/CNF green nanocomposites containing 3 wt% CNFs	1066:1115	TPS/CNF green nanocomposites containing 3 wt% CNFs had exceptional tensile strength (~161%), tensile modulus (~167%), thermal stability, and crystallinity.
34582904	5	15	from	nanofibers	782:791	arg1	concentrations					806:819	different concentrations	796:819	different concentrations (0, 1, 2, 3, 4 wt%)	796:839	Then, cellulose nanofibers in different concentrations (0, 1, 2, 3, 4 wt%) were added to the thermoplastic starch matrix.
34582904	5	15	from	nanofibers	782:791	arg1	%					838:838	0, 1, 2, 3, 4 wt%	822:838	0, 1, 2, 3, 4 wt%	822:838	Then, cellulose nanofibers in different concentrations (0, 1, 2, 3, 4 wt%) were added to the thermoplastic starch matrix.
34582904	5	16	theme	different	796:804	arg1	concentrations					806:819	different concentrations	796:819	different concentrations (0, 1, 2, 3, 4 wt%)	796:839	Then, cellulose nanofibers in different concentrations (0, 1, 2, 3, 4 wt%) were added to the thermoplastic starch matrix.
34582904	5	16	theme	different	796:804	arg1	%					838:838	0, 1, 2, 3, 4 wt%	822:838	0, 1, 2, 3, 4 wt%	822:838	Then, cellulose nanofibers in different concentrations (0, 1, 2, 3, 4 wt%) were added to the thermoplastic starch matrix.
34582904	4	17	theme	green	610:614	arg1	films					630:634	green nanocomposite films	610:634	green nanocomposite films	610:634	Following that, green nanocomposite films were made from potato starch using the solution casting process, by which we used glycerol (30 wt%) to make thermoplastic starch.
34582904	1	18	theme	thermoplastic	99:111	arg1	films					140:144	Green thermoplastic starch (TPS) nanocomposite films	93:144	Green thermoplastic starch (TPS) nanocomposite films aided by cellulose nanofibers (CNFs) from Chrysopogon zizanioides roots	93:216	Green thermoplastic starch (TPS) nanocomposite films aided by cellulose nanofibers (CNFs) from Chrysopogon zizanioides roots were developed and characterized.
34582904	7	19	theme	crystallinity	998:1010	arg1	index					1012:1016	a very high crystallinity index	986:1016	a very high crystallinity index (~65%)	986:1023	Besides, these nanostructures presented a very high crystallinity index (~65%), thereby enhanced the thermal stability.
34582904	7	19	theme	crystallinity	998:1010	arg1	%					1022:1022	~65%	1019:1022	~65%	1019:1022	Besides, these nanostructures presented a very high crystallinity index (~65%), thereby enhanced the thermal stability.
34582904	0	20	theme	zizanioides	69:79	arg1	nanofibers					81:90	cellulose-rich Chrysopogon zizanioides nanofibers	42:90	cellulose-rich Chrysopogon zizanioides nanofibers	42:90	Thermoplastic starch nanocomposites using cellulose-rich Chrysopogon zizanioides nanofibers.
34582904	1	21	theme	starch	113:118	arg1	films					140:144	Green thermoplastic starch (TPS) nanocomposite films	93:144	Green thermoplastic starch (TPS) nanocomposite films aided by cellulose nanofibers (CNFs) from Chrysopogon zizanioides roots	93:216	Green thermoplastic starch (TPS) nanocomposite films aided by cellulose nanofibers (CNFs) from Chrysopogon zizanioides roots were developed and characterized.
34582904	1	22	theme	Chrysopogon	188:198	arg1	roots					212:216	Chrysopogon zizanioides roots	188:216	Chrysopogon zizanioides roots	188:216	Green thermoplastic starch (TPS) nanocomposite films aided by cellulose nanofibers (CNFs) from Chrysopogon zizanioides roots were developed and characterized.
34582904	3	23	theme	environmentally	412:426	arg1	technique					452:460	an environmentally friendly acid isolation technique	409:460	an environmentally friendly acid isolation technique that included three stages: (i) alkali treatment; (ii) bleaching; and (iii) mild acid hydrolysis using oxalic acid in an autoclave	409:591	CNFs were separated using an environmentally friendly acid isolation technique that included three stages: (i) alkali treatment; (ii) bleaching; and (iii) mild acid hydrolysis using oxalic acid in an autoclave.
34582904	8	24	theme	green	1074:1078	arg1	nanocomposites					1080:1093	TPS/CNF green nanocomposites	1066:1093	TPS/CNF green nanocomposites containing 3 wt% CNFs	1066:1115	TPS/CNF green nanocomposites containing 3 wt% CNFs had exceptional tensile strength (~161%), tensile modulus (~167%), thermal stability, and crystallinity.
34582904	10	25	theme	high	1457:1460	arg1	potential					1462:1470	high potential	1457:1470	high potential for polymer industry	1457:1491	It can be concluded that Chrysopogon zizanioides roots have high potential for polymer industry.
34582904	1	26	theme	zizanioides	200:210	arg1	roots					212:216	Chrysopogon zizanioides roots	188:216	Chrysopogon zizanioides roots	188:216	Green thermoplastic starch (TPS) nanocomposite films aided by cellulose nanofibers (CNFs) from Chrysopogon zizanioides roots were developed and characterized.
34582904	0	27	theme	Thermoplastic	0:12	arg1	starch					14:19	Thermoplastic starch	0:19	Thermoplastic starch	0:19	Thermoplastic starch nanocomposites using cellulose-rich Chrysopogon zizanioides nanofibers.
34582904	10	28	theme	polymer	1476:1482	arg1	industry					1484:1491	polymer industry	1476:1491	polymer industry	1476:1491	It can be concluded that Chrysopogon zizanioides roots have high potential for polymer industry.
34582904	7	29	theme	high	993:996	arg1	index					1012:1016	a very high crystallinity index	986:1016	a very high crystallinity index (~65%)	986:1023	Besides, these nanostructures presented a very high crystallinity index (~65%), thereby enhanced the thermal stability.
34582904	7	29	theme	high	993:996	arg1	%					1022:1022	~65%	1019:1022	~65%	1019:1022	Besides, these nanostructures presented a very high crystallinity index (~65%), thereby enhanced the thermal stability.
34582904	2	30	theme	cellulose	357:365	arg1	%					379:379	~48%	376:379	~48%	376:379	When compared to other lignocellulosic fibers, Chrysopogon zizanioides roots revealed exceptionally high cellulose content (~48%).
34582904	2	30	theme	cellulose	357:365	arg1	content					367:373	exceptionally high cellulose content	338:373	exceptionally high cellulose content (~48%)	338:380	When compared to other lignocellulosic fibers, Chrysopogon zizanioides roots revealed exceptionally high cellulose content (~48%).
34582904	3	31	from	acid	572:575	arg1	autoclave					583:591	an autoclave	580:591	an autoclave	580:591	CNFs were separated using an environmentally friendly acid isolation technique that included three stages: (i) alkali treatment; (ii) bleaching; and (iii) mild acid hydrolysis using oxalic acid in an autoclave.
34582904	4	32	theme	potato	651:656	arg1	starch					658:663	potato starch	651:663	potato starch using the solution casting process, by which we used glycerol (30 wt%) to make thermoplastic starch	651:763	Following that, green nanocomposite films were made from potato starch using the solution casting process, by which we used glycerol (30 wt%) to make thermoplastic starch.
34582904	5	33	theme	cellulose	772:780	arg1	nanofibers					782:791	cellulose nanofibers	772:791	cellulose nanofibers in different concentrations (0, 1, 2, 3, 4 wt%)	772:839	Then, cellulose nanofibers in different concentrations (0, 1, 2, 3, 4 wt%) were added to the thermoplastic starch matrix.
34582904	2	34	theme	high	352:355	arg1	%					379:379	~48%	376:379	~48%	376:379	When compared to other lignocellulosic fibers, Chrysopogon zizanioides roots revealed exceptionally high cellulose content (~48%).
34582904	2	34	theme	high	352:355	arg1	content					367:373	exceptionally high cellulose content	338:373	exceptionally high cellulose content (~48%)	338:380	When compared to other lignocellulosic fibers, Chrysopogon zizanioides roots revealed exceptionally high cellulose content (~48%).
34582904	10	35	contain	have	1452:1455	arg2	potential					1462:1470	high potential	1457:1470	high potential for polymer industry	1457:1491	It can be concluded that Chrysopogon zizanioides roots have high potential for polymer industry.
34582904	10	35	contain	have	1452:1455	arg1	roots					1446:1450	Chrysopogon zizanioides roots	1422:1450	Chrysopogon zizanioides roots	1422:1450	It can be concluded that Chrysopogon zizanioides roots have high potential for polymer industry.
34582904	4	36	theme	nanocomposite	616:628	arg1	films					630:634	green nanocomposite films	610:634	green nanocomposite films	610:634	Following that, green nanocomposite films were made from potato starch using the solution casting process, by which we used glycerol (30 wt%) to make thermoplastic starch.
34582904	1	37	theme	TPS	121:123	arg1	films					140:144	Green thermoplastic starch (TPS) nanocomposite films	93:144	Green thermoplastic starch (TPS) nanocomposite films aided by cellulose nanofibers (CNFs) from Chrysopogon zizanioides roots	93:216	Green thermoplastic starch (TPS) nanocomposite films aided by cellulose nanofibers (CNFs) from Chrysopogon zizanioides roots were developed and characterized.
34582904	2	38	theme	zizanioides	311:321	arg1	roots					323:327	Chrysopogon zizanioides roots	299:327	Chrysopogon zizanioides roots	299:327	When compared to other lignocellulosic fibers, Chrysopogon zizanioides roots revealed exceptionally high cellulose content (~48%).
34582904	1	39	theme	nanocomposite	126:138	arg1	films					140:144	Green thermoplastic starch (TPS) nanocomposite films	93:144	Green thermoplastic starch (TPS) nanocomposite films aided by cellulose nanofibers (CNFs) from Chrysopogon zizanioides roots	93:216	Green thermoplastic starch (TPS) nanocomposite films aided by cellulose nanofibers (CNFs) from Chrysopogon zizanioides roots were developed and characterized.
34582904	3	40	theme	alkali	494:499	arg1	treatment					501:509	(i) alkali treatment	490:509	(i) alkali treatment	490:509	CNFs were separated using an environmentally friendly acid isolation technique that included three stages: (i) alkali treatment; (ii) bleaching; and (iii) mild acid hydrolysis using oxalic acid in an autoclave.
34582904	0	41	theme	Chrysopogon	57:67	arg1	nanofibers					81:90	cellulose-rich Chrysopogon zizanioides nanofibers	42:90	cellulose-rich Chrysopogon zizanioides nanofibers	42:90	Thermoplastic starch nanocomposites using cellulose-rich Chrysopogon zizanioides nanofibers.
34582904	9	42	theme	Chrysopogon	1318:1328	arg1	roots					1342:1346	Chrysopogon zizanioides roots	1318:1346	Chrysopogon zizanioides roots	1318:1346	As a result, nanocomposite films made of starch and cellulose nanofibers (3 wt%) extracted from Chrysopogon zizanioides roots would be alternatives for sustainable packaging.
34582904	8	43	contain	had	1117:1119	arg2	modulus					1167:1173	tensile modulus	1159:1173	tensile modulus (~167%)	1159:1181	TPS/CNF green nanocomposites containing 3 wt% CNFs had exceptional tensile strength (~161%), tensile modulus (~167%), thermal stability, and crystallinity.
34582904	8	43	contain	had	1117:1119	arg2	%					1180:1180	~167%	1176:1180	~167%	1176:1180	TPS/CNF green nanocomposites containing 3 wt% CNFs had exceptional tensile strength (~161%), tensile modulus (~167%), thermal stability, and crystallinity.
34582904	8	43	contain	had	1117:1119	arg2	%					1155:1155	~161%	1151:1155	~161%	1151:1155	TPS/CNF green nanocomposites containing 3 wt% CNFs had exceptional tensile strength (~161%), tensile modulus (~167%), thermal stability, and crystallinity.
34582904	8	43	contain	had	1117:1119	arg2	crystallinity					1207:1219	crystallinity	1207:1219	crystallinity	1207:1219	TPS/CNF green nanocomposites containing 3 wt% CNFs had exceptional tensile strength (~161%), tensile modulus (~167%), thermal stability, and crystallinity.
34582904	8	43	contain	had	1117:1119	arg2	stability					1192:1200	thermal stability	1184:1200	thermal stability	1184:1200	TPS/CNF green nanocomposites containing 3 wt% CNFs had exceptional tensile strength (~161%), tensile modulus (~167%), thermal stability, and crystallinity.
34582904	8	43	contain	had	1117:1119	arg2	strength					1141:1148	exceptional tensile strength	1121:1148	exceptional tensile strength (~161%)	1121:1156	TPS/CNF green nanocomposites containing 3 wt% CNFs had exceptional tensile strength (~161%), tensile modulus (~167%), thermal stability, and crystallinity.
34582904	8	43	contain	had	1117:1119	arg1	nanocomposites					1080:1093	TPS/CNF green nanocomposites	1066:1093	TPS/CNF green nanocomposites containing 3 wt% CNFs	1066:1115	TPS/CNF green nanocomposites containing 3 wt% CNFs had exceptional tensile strength (~161%), tensile modulus (~167%), thermal stability, and crystallinity.
34582904	8	44	theme	tensile	1159:1165	arg1	modulus					1167:1173	tensile modulus	1159:1173	tensile modulus (~167%)	1159:1181	TPS/CNF green nanocomposites containing 3 wt% CNFs had exceptional tensile strength (~161%), tensile modulus (~167%), thermal stability, and crystallinity.
34582904	8	44	theme	tensile	1159:1165	arg1	%					1180:1180	~167%	1176:1180	~167%	1176:1180	TPS/CNF green nanocomposites containing 3 wt% CNFs had exceptional tensile strength (~161%), tensile modulus (~167%), thermal stability, and crystallinity.
34582904	0	45	theme	cellulose-rich	42:55	arg1	nanofibers					81:90	cellulose-rich Chrysopogon zizanioides nanofibers	42:90	cellulose-rich Chrysopogon zizanioides nanofibers	42:90	Thermoplastic starch nanocomposites using cellulose-rich Chrysopogon zizanioides nanofibers.
34582904	8	46	theme	%	1110:1110	arg1	CNFs					1112:1115	3 wt% CNFs	1106:1115	3 wt% CNFs	1106:1115	TPS/CNF green nanocomposites containing 3 wt% CNFs had exceptional tensile strength (~161%), tensile modulus (~167%), thermal stability, and crystallinity.
34582904	7	47	theme	thermal	1047:1053	arg1	stability					1055:1063	the thermal stability	1043:1063	the thermal stability	1043:1063	Besides, these nanostructures presented a very high crystallinity index (~65%), thereby enhanced the thermal stability.
34582904	6	48	theme	17-27 nm	936:943	arg1	range					927:931	the range	923:931	the range of 17-27 nm	923:943	The isolated CNFs had diameters in the range of 17-27 nm.
34582904	3	49	theme	acid	437:440	arg1	technique					452:460	an environmentally friendly acid isolation technique	409:460	an environmentally friendly acid isolation technique that included three stages: (i) alkali treatment; (ii) bleaching; and (iii) mild acid hydrolysis using oxalic acid in an autoclave	409:591	CNFs were separated using an environmentally friendly acid isolation technique that included three stages: (i) alkali treatment; (ii) bleaching; and (iii) mild acid hydrolysis using oxalic acid in an autoclave.
34582904	3	50	theme	isolation	442:450	arg1	technique					452:460	an environmentally friendly acid isolation technique	409:460	an environmentally friendly acid isolation technique that included three stages: (i) alkali treatment; (ii) bleaching; and (iii) mild acid hydrolysis using oxalic acid in an autoclave	409:591	CNFs were separated using an environmentally friendly acid isolation technique that included three stages: (i) alkali treatment; (ii) bleaching; and (iii) mild acid hydrolysis using oxalic acid in an autoclave.
34582904	3	51	dep	bleaching	517:525	arg1	ii					513:514	ii	513:514	ii	513:514	CNFs were separated using an environmentally friendly acid isolation technique that included three stages: (i) alkali treatment; (ii) bleaching; and (iii) mild acid hydrolysis using oxalic acid in an autoclave.
34582904	8	52	contain	containing	1095:1104	arg2	CNFs					1112:1115	3 wt% CNFs	1106:1115	3 wt% CNFs	1106:1115	TPS/CNF green nanocomposites containing 3 wt% CNFs had exceptional tensile strength (~161%), tensile modulus (~167%), thermal stability, and crystallinity.
34582904	8	52	contain	containing	1095:1104	arg1	nanocomposites					1080:1093	TPS/CNF green nanocomposites	1066:1093	TPS/CNF green nanocomposites containing 3 wt% CNFs	1066:1115	TPS/CNF green nanocomposites containing 3 wt% CNFs had exceptional tensile strength (~161%), tensile modulus (~167%), thermal stability, and crystallinity.
34582904	4	53	theme	thermoplastic	744:756	arg1	starch					758:763	thermoplastic starch	744:763	thermoplastic starch	744:763	Following that, green nanocomposite films were made from potato starch using the solution casting process, by which we used glycerol (30 wt%) to make thermoplastic starch.
34582904	9	54	theme	sustainable	1374:1384	arg1	packaging					1386:1394	sustainable packaging	1374:1394	sustainable packaging	1374:1394	As a result, nanocomposite films made of starch and cellulose nanofibers (3 wt%) extracted from Chrysopogon zizanioides roots would be alternatives for sustainable packaging.
34582904	3	55	theme	acid	543:546	arg1	hydrolysis					548:557	(iii) mild acid hydrolysis	532:557	(iii) mild acid hydrolysis	532:557	CNFs were separated using an environmentally friendly acid isolation technique that included three stages: (i) alkali treatment; (ii) bleaching; and (iii) mild acid hydrolysis using oxalic acid in an autoclave.
34582904	9	56	theme	nanocomposite	1235:1247	arg1	films					1249:1253	nanocomposite films	1235:1253	nanocomposite films made of starch and cellulose nanofibers (3 wt%) extracted from Chrysopogon zizanioides roots	1235:1346	As a result, nanocomposite films made of starch and cellulose nanofibers (3 wt%) extracted from Chrysopogon zizanioides roots would be alternatives for sustainable packaging.
34582904	2	57	theme	Chrysopogon	299:309	arg1	roots					323:327	Chrysopogon zizanioides roots	299:327	Chrysopogon zizanioides roots	299:327	When compared to other lignocellulosic fibers, Chrysopogon zizanioides roots revealed exceptionally high cellulose content (~48%).
34582904	5	58	theme	starch	873:878	arg1	matrix					880:885	the thermoplastic starch matrix	855:885	the thermoplastic starch matrix	855:885	Then, cellulose nanofibers in different concentrations (0, 1, 2, 3, 4 wt%) were added to the thermoplastic starch matrix.
34582904	10	59	theme	Chrysopogon	1422:1432	arg1	roots					1446:1450	Chrysopogon zizanioides roots	1422:1450	Chrysopogon zizanioides roots	1422:1450	It can be concluded that Chrysopogon zizanioides roots have high potential for polymer industry.
34582904	1	60	theme	cellulose	155:163	arg1	CNFs					177:180	CNFs	177:180	CNFs	177:180	Green thermoplastic starch (TPS) nanocomposite films aided by cellulose nanofibers (CNFs) from Chrysopogon zizanioides roots were developed and characterized.
34582904	1	60	theme	cellulose	155:163	arg1	nanofibers					165:174	cellulose nanofibers	155:174	cellulose nanofibers (CNFs) from Chrysopogon zizanioides roots	155:216	Green thermoplastic starch (TPS) nanocomposite films aided by cellulose nanofibers (CNFs) from Chrysopogon zizanioides roots were developed and characterized.
34582904	9	61	theme	zizanioides	1330:1340	arg1	roots					1342:1346	Chrysopogon zizanioides roots	1318:1346	Chrysopogon zizanioides roots	1318:1346	As a result, nanocomposite films made of starch and cellulose nanofibers (3 wt%) extracted from Chrysopogon zizanioides roots would be alternatives for sustainable packaging.
34582904	6	62	contain	had	906:908	arg2	diameters					910:918	diameters	910:918	diameters	910:918	The isolated CNFs had diameters in the range of 17-27 nm.
34582904	6	62	contain	had	906:908	arg1	CNFs					901:904	The isolated CNFs	888:904	The isolated CNFs	888:904	The isolated CNFs had diameters in the range of 17-27 nm.
34582904	2	63	theme	lignocellulosic	275:289	arg1	fibers					291:296	other lignocellulosic fibers	269:296	other lignocellulosic fibers	269:296	When compared to other lignocellulosic fibers, Chrysopogon zizanioides roots revealed exceptionally high cellulose content (~48%).
34582904	1	64	from	roots	212:216	arg1	CNFs					177:180	CNFs	177:180	CNFs	177:180	Green thermoplastic starch (TPS) nanocomposite films aided by cellulose nanofibers (CNFs) from Chrysopogon zizanioides roots were developed and characterized.
34582904	1	64	from	roots	212:216	arg1	nanofibers					165:174	cellulose nanofibers	155:174	cellulose nanofibers (CNFs) from Chrysopogon zizanioides roots	155:216	Green thermoplastic starch (TPS) nanocomposite films aided by cellulose nanofibers (CNFs) from Chrysopogon zizanioides roots were developed and characterized.
34582904	5	65	theme	thermoplastic	859:871	arg1	matrix					880:885	the thermoplastic starch matrix	855:885	the thermoplastic starch matrix	855:885	Then, cellulose nanofibers in different concentrations (0, 1, 2, 3, 4 wt%) were added to the thermoplastic starch matrix.
34582904	8	66	theme	tensile	1133:1139	arg1	strength					1141:1148	exceptional tensile strength	1121:1148	exceptional tensile strength (~161%)	1121:1156	TPS/CNF green nanocomposites containing 3 wt% CNFs had exceptional tensile strength (~161%), tensile modulus (~167%), thermal stability, and crystallinity.
34582904	8	66	theme	tensile	1133:1139	arg1	%					1155:1155	~161%	1151:1155	~161%	1151:1155	TPS/CNF green nanocomposites containing 3 wt% CNFs had exceptional tensile strength (~161%), tensile modulus (~167%), thermal stability, and crystallinity.
34582904	8	67	theme	exceptional	1121:1131	arg1	strength					1141:1148	exceptional tensile strength	1121:1148	exceptional tensile strength (~161%)	1121:1156	TPS/CNF green nanocomposites containing 3 wt% CNFs had exceptional tensile strength (~161%), tensile modulus (~167%), thermal stability, and crystallinity.
34582904	8	67	theme	exceptional	1121:1131	arg1	%					1155:1155	~161%	1151:1155	~161%	1151:1155	TPS/CNF green nanocomposites containing 3 wt% CNFs had exceptional tensile strength (~161%), tensile modulus (~167%), thermal stability, and crystallinity.
34582904	2	68	theme	other	269:273	arg1	fibers					291:296	other lignocellulosic fibers	269:296	other lignocellulosic fibers	269:296	When compared to other lignocellulosic fibers, Chrysopogon zizanioides roots revealed exceptionally high cellulose content (~48%).
34582904	3	69	theme	oxalic	565:570	arg1	acid					572:575	oxalic acid	565:575	oxalic acid in an autoclave	565:591	CNFs were separated using an environmentally friendly acid isolation technique that included three stages: (i) alkali treatment; (ii) bleaching; and (iii) mild acid hydrolysis using oxalic acid in an autoclave.
31926225	0	0	theme	intestinal	88:97	arg1	targeting					99:107	intestinal targeting	88:107	intestinal targeting of nutraceuticals	88:125	Novel pH-sensitive alginate hydrogel delivery system reinforced with gum tragacanth for intestinal targeting of nutraceuticals.
31926225	10	1	theme	delivery	1146:1153	arg1	system					1155:1160	a promising delivery system	1134:1160	a promising delivery system for drugs and nutraceutical compounds	1134:1198	Thus our findings suggest SA-GT gel formulation could be used as a promising delivery system for drugs and nutraceutical compounds.
31926225	8	2	theme	SA	964:965	arg1	behavior					952:959	the thermal behavior	940:959	the thermal behavior of SA	940:965	TGA analysis revealed that GT incorporation did not affect the thermal behavior of SA.
31926225	6	3	dep	loss	778:781	arg1	%					769:769	%	769:769	%	769:769	GT incorporated hydrogel beads exhibited increased biodegradation (up to 82% weight loss) in biodegradation media (in vitro).
31926225	10	4	theme	gel	1101:1103	arg1	formulation					1105:1115	SA-GT gel formulation	1095:1115	SA-GT gel formulation	1095:1115	Thus our findings suggest SA-GT gel formulation could be used as a promising delivery system for drugs and nutraceutical compounds.
31926225	5	5	theme	encapsulation	616:628	arg1	efficiency					630:639	increased encapsulation efficiency	606:639	increased encapsulation efficiency	606:639	SA-GT composite hydrogels revealed increased encapsulation efficiency with sustained release of phenolic compounds in SIF.
31926225	2	6	theme	Basella	343:349	arg1	sps					351:353	Basella sps	343:353	Basella sps	343:353	The composite hydrogel beads were encapsulated with phenolic compounds extracted from Basella sps.
31926225	1	7	theme	SA	238:239	arg1	beads					250:254	SA hydrogel beads	238:254	SA hydrogel beads	238:254	The present study utilizes the novel combination of Gum tragacanth (GT) and sodium alginate (SA) to reinforce SA hydrogel beads.
31926225	9	8	theme	SA-GT	981:985	arg1	hydrogels					1000:1008	SA-GT encapsulated hydrogels	981:1008	SA-GT encapsulated hydrogels	981:1008	Furthermore, SA-GT encapsulated hydrogels showed remarkable cytotoxicity against osteosarcoma cells.
31926225	2	9	theme	hydrogel	271:278	arg1	beads					280:284	The composite hydrogel beads	257:284	The composite hydrogel beads	257:284	The composite hydrogel beads were encapsulated with phenolic compounds extracted from Basella sps.
31926225	1	10	theme	hydrogel	241:248	arg1	beads					250:254	SA hydrogel beads	238:254	SA hydrogel beads	238:254	The present study utilizes the novel combination of Gum tragacanth (GT) and sodium alginate (SA) to reinforce SA hydrogel beads.
31926225	4	11	theme	simulated	491:499	arg1	SIF					519:521	SIF	519:521	SIF	519:521	Higher swelling behavior was observed in simulated intestinal fluid (SIF) with increasing GT content in SA formulations.
31926225	4	11	theme	simulated	491:499	arg1	fluid					512:516	simulated intestinal fluid	491:516	simulated intestinal fluid (SIF)	491:522	Higher swelling behavior was observed in simulated intestinal fluid (SIF) with increasing GT content in SA formulations.
31926225	2	12	theme	composite	261:269	arg1	beads					280:284	The composite hydrogel beads	257:284	The composite hydrogel beads	257:284	The composite hydrogel beads were encapsulated with phenolic compounds extracted from Basella sps.
31926225	8	13	theme	TGA	881:883	arg1	analysis					885:892	TGA analysis	881:892	TGA analysis	881:892	TGA analysis revealed that GT incorporation did not affect the thermal behavior of SA.
31926225	5	14	theme	sustained	646:654	arg1	release					656:662	sustained release	646:662	sustained release of phenolic compounds in SIF	646:691	SA-GT composite hydrogels revealed increased encapsulation efficiency with sustained release of phenolic compounds in SIF.
31926225	6	15	dep	media	802:806	arg1	vitro					812:816	in vitro	809:816	in vitro	809:816	GT incorporated hydrogel beads exhibited increased biodegradation (up to 82% weight loss) in biodegradation media (in vitro).
31926225	1	16	theme	Gum	180:182	arg1	GT					196:197	GT	196:197	GT	196:197	The present study utilizes the novel combination of Gum tragacanth (GT) and sodium alginate (SA) to reinforce SA hydrogel beads.
31926225	1	16	theme	Gum	180:182	arg1	tragacanth					184:193	Gum tragacanth	180:193	Gum tragacanth (GT)	180:198	The present study utilizes the novel combination of Gum tragacanth (GT) and sodium alginate (SA) to reinforce SA hydrogel beads.
31926225	0	17	theme	nutraceuticals	112:125	arg1	targeting					99:107	intestinal targeting	88:107	intestinal targeting of nutraceuticals	88:125	Novel pH-sensitive alginate hydrogel delivery system reinforced with gum tragacanth for intestinal targeting of nutraceuticals.
31926225	5	18	theme	composite	577:585	arg1	hydrogels					587:595	SA-GT composite hydrogels	571:595	SA-GT composite hydrogels	571:595	SA-GT composite hydrogels revealed increased encapsulation efficiency with sustained release of phenolic compounds in SIF.
31926225	3	19	theme	increased	390:398	arg1	property					408:415	increased elastic property	390:415	increased elastic property of GT incorporated formulations	390:447	The rheological studies conferred increased elastic property of GT incorporated formulations.
31926225	1	20	theme	tragacanth	184:193	arg1	combination					165:175	the novel combination	155:175	the novel combination of Gum tragacanth (GT) and sodium alginate (SA)	155:223	The present study utilizes the novel combination of Gum tragacanth (GT) and sodium alginate (SA) to reinforce SA hydrogel beads.
31926225	0	21	theme	pH-sensitive	6:17	arg1	system					46:51	Novel pH-sensitive alginate hydrogel delivery system	0:51	Novel pH-sensitive alginate hydrogel delivery system	0:51	Novel pH-sensitive alginate hydrogel delivery system reinforced with gum tragacanth for intestinal targeting of nutraceuticals.
31926225	4	22	theme	intestinal	501:510	arg1	SIF					519:521	SIF	519:521	SIF	519:521	Higher swelling behavior was observed in simulated intestinal fluid (SIF) with increasing GT content in SA formulations.
31926225	4	22	theme	intestinal	501:510	arg1	fluid					512:516	simulated intestinal fluid	491:516	simulated intestinal fluid (SIF)	491:522	Higher swelling behavior was observed in simulated intestinal fluid (SIF) with increasing GT content in SA formulations.
31926225	5	23	theme	phenolic	667:674	arg1	compounds					676:684	phenolic compounds	667:684	phenolic compounds	667:684	SA-GT composite hydrogels revealed increased encapsulation efficiency with sustained release of phenolic compounds in SIF.
31926225	6	24	theme	increased	735:743	arg1	biodegradation					745:758	increased biodegradation	735:758	increased biodegradation (up to 82% weight loss)	735:782	GT incorporated hydrogel beads exhibited increased biodegradation (up to 82% weight loss) in biodegradation media (in vitro).
31926225	6	24	theme	increased	735:743	arg1	loss					778:781	up to 82% weight loss	761:781	up to 82% weight loss	761:781	GT incorporated hydrogel beads exhibited increased biodegradation (up to 82% weight loss) in biodegradation media (in vitro).
31926225	3	25	theme	incorporated	423:434	arg1	formulations					436:447	GT incorporated formulations	420:447	GT incorporated formulations	420:447	The rheological studies conferred increased elastic property of GT incorporated formulations.
31926225	0	26	theme	Novel	0:4	arg1	system					46:51	Novel pH-sensitive alginate hydrogel delivery system	0:51	Novel pH-sensitive alginate hydrogel delivery system	0:51	Novel pH-sensitive alginate hydrogel delivery system reinforced with gum tragacanth for intestinal targeting of nutraceuticals.
31926225	7	27	theme	FTIR	820:823	arg1	study					825:829	FTIR study	820:829	FTIR study	820:829	FTIR study found no molecular interaction between SA and GT.
31926225	4	28	from	content	543:549	arg1	formulations					557:568	SA formulations	554:568	SA formulations	554:568	Higher swelling behavior was observed in simulated intestinal fluid (SIF) with increasing GT content in SA formulations.
31926225	7	29	theme	molecular	840:848	arg1	interaction					850:860	no molecular interaction	837:860	no molecular interaction between SA and GT	837:878	FTIR study found no molecular interaction between SA and GT.
31926225	3	30	theme	formulations	436:447	arg1	property					408:415	increased elastic property	390:415	increased elastic property of GT incorporated formulations	390:447	The rheological studies conferred increased elastic property of GT incorporated formulations.
31926225	0	31	theme	hydrogel	28:35	arg1	system					46:51	Novel pH-sensitive alginate hydrogel delivery system	0:51	Novel pH-sensitive alginate hydrogel delivery system	0:51	Novel pH-sensitive alginate hydrogel delivery system reinforced with gum tragacanth for intestinal targeting of nutraceuticals.
31926225	4	32	theme	SA	554:555	arg1	formulations					557:568	SA formulations	554:568	SA formulations	554:568	Higher swelling behavior was observed in simulated intestinal fluid (SIF) with increasing GT content in SA formulations.
31926225	5	33	theme	increased	606:614	arg1	efficiency					630:639	increased encapsulation efficiency	606:639	increased encapsulation efficiency	606:639	SA-GT composite hydrogels revealed increased encapsulation efficiency with sustained release of phenolic compounds in SIF.
31926225	0	34	theme	alginate	19:26	arg1	system					46:51	Novel pH-sensitive alginate hydrogel delivery system	0:51	Novel pH-sensitive alginate hydrogel delivery system	0:51	Novel pH-sensitive alginate hydrogel delivery system reinforced with gum tragacanth for intestinal targeting of nutraceuticals.
31926225	5	35	from	release	656:662	arg1	SIF					689:691	SIF	689:691	SIF	689:691	SA-GT composite hydrogels revealed increased encapsulation efficiency with sustained release of phenolic compounds in SIF.
31926225	10	36	dep	suggest	1087:1093	arg1	used					1126:1129	used	1126:1129	suggest SA-GT gel formulation could be used as a promising delivery system for drugs and nutraceutical compounds	1087:1198	Thus our findings suggest SA-GT gel formulation could be used as a promising delivery system for drugs and nutraceutical compounds.
31926225	6	37	theme	hydrogel	710:717	arg1	beads					719:723	hydrogel beads	710:723	hydrogel beads exhibited increased biodegradation (up to 82% weight loss)	710:782	GT incorporated hydrogel beads exhibited increased biodegradation (up to 82% weight loss) in biodegradation media (in vitro).
31926225	8	38	theme	GT	908:909	arg1	incorporation					911:923	GT incorporation	908:923	GT incorporation	908:923	TGA analysis revealed that GT incorporation did not affect the thermal behavior of SA.
31926225	10	39	theme	promising	1136:1144	arg1	system					1155:1160	a promising delivery system	1134:1160	a promising delivery system for drugs and nutraceutical compounds	1134:1198	Thus our findings suggest SA-GT gel formulation could be used as a promising delivery system for drugs and nutraceutical compounds.
31926225	4	40	theme	swelling	457:464	arg1	behavior					466:473	Higher swelling behavior	450:473	Higher swelling behavior	450:473	Higher swelling behavior was observed in simulated intestinal fluid (SIF) with increasing GT content in SA formulations.
31926225	4	41	located	observed	479:486	arg1	SIF					519:521	SIF	519:521	SIF	519:521	Higher swelling behavior was observed in simulated intestinal fluid (SIF) with increasing GT content in SA formulations.
31926225	4	41	located	observed	479:486	arg2	behavior					466:473	Higher swelling behavior	450:473	Higher swelling behavior	450:473	Higher swelling behavior was observed in simulated intestinal fluid (SIF) with increasing GT content in SA formulations.
31926225	4	41	located	observed	479:486	arg1	fluid					512:516	simulated intestinal fluid	491:516	simulated intestinal fluid (SIF)	491:522	Higher swelling behavior was observed in simulated intestinal fluid (SIF) with increasing GT content in SA formulations.
31926225	1	42	theme	sodium	204:209	arg1	SA					221:222	SA	221:222	SA	221:222	The present study utilizes the novel combination of Gum tragacanth (GT) and sodium alginate (SA) to reinforce SA hydrogel beads.
31926225	1	42	theme	sodium	204:209	arg1	alginate					211:218	sodium alginate	204:218	sodium alginate (SA)	204:223	The present study utilizes the novel combination of Gum tragacanth (GT) and sodium alginate (SA) to reinforce SA hydrogel beads.
31926225	0	43	theme	delivery	37:44	arg1	system					46:51	Novel pH-sensitive alginate hydrogel delivery system	0:51	Novel pH-sensitive alginate hydrogel delivery system	0:51	Novel pH-sensitive alginate hydrogel delivery system reinforced with gum tragacanth for intestinal targeting of nutraceuticals.
31926225	4	44	theme	GT	540:541	arg1	content					543:549	GT content	540:549	GT content in SA formulations	540:568	Higher swelling behavior was observed in simulated intestinal fluid (SIF) with increasing GT content in SA formulations.
31926225	3	45	theme	GT	420:421	arg1	formulations					436:447	GT incorporated formulations	420:447	GT incorporated formulations	420:447	The rheological studies conferred increased elastic property of GT incorporated formulations.
31926225	3	46	theme	rheological	360:370	arg1	studies					372:378	The rheological studies	356:378	The rheological studies	356:378	The rheological studies conferred increased elastic property of GT incorporated formulations.
31926225	6	47	theme	biodegradation	787:800	arg1	media					802:806	biodegradation media	787:806	biodegradation media (in vitro)	787:817	GT incorporated hydrogel beads exhibited increased biodegradation (up to 82% weight loss) in biodegradation media (in vitro).
31926225	1	48	theme	alginate	211:218	arg1	combination					165:175	the novel combination	155:175	the novel combination of Gum tragacanth (GT) and sodium alginate (SA)	155:223	The present study utilizes the novel combination of Gum tragacanth (GT) and sodium alginate (SA) to reinforce SA hydrogel beads.
31926225	3	49	theme	elastic	400:406	arg1	property					408:415	increased elastic property	390:415	increased elastic property of GT incorporated formulations	390:447	The rheological studies conferred increased elastic property of GT incorporated formulations.
31926225	10	50	theme	nutraceutical	1176:1188	arg1	compounds					1190:1198	nutraceutical compounds	1176:1198	nutraceutical compounds	1176:1198	Thus our findings suggest SA-GT gel formulation could be used as a promising delivery system for drugs and nutraceutical compounds.
31926225	10	51	theme	SA-GT	1095:1099	arg1	formulation					1105:1115	SA-GT gel formulation	1095:1115	SA-GT gel formulation	1095:1115	Thus our findings suggest SA-GT gel formulation could be used as a promising delivery system for drugs and nutraceutical compounds.
31926225	8	52	theme	thermal	944:950	arg1	behavior					952:959	the thermal behavior	940:959	the thermal behavior of SA	940:965	TGA analysis revealed that GT incorporation did not affect the thermal behavior of SA.
31926225	1	53	theme	present	132:138	arg1	study					140:144	The present study	128:144	The present study	128:144	The present study utilizes the novel combination of Gum tragacanth (GT) and sodium alginate (SA) to reinforce SA hydrogel beads.
31926225	6	54	dep	82	767:768	arg1	to					764:765	to	764:765	to	764:765	GT incorporated hydrogel beads exhibited increased biodegradation (up to 82% weight loss) in biodegradation media (in vitro).
31926225	5	55	theme	SA-GT	571:575	arg1	hydrogels					587:595	SA-GT composite hydrogels	571:595	SA-GT composite hydrogels	571:595	SA-GT composite hydrogels revealed increased encapsulation efficiency with sustained release of phenolic compounds in SIF.
31926225	9	56	theme	osteosarcoma	1049:1060	arg1	cells					1062:1066	osteosarcoma cells	1049:1066	osteosarcoma cells	1049:1066	Furthermore, SA-GT encapsulated hydrogels showed remarkable cytotoxicity against osteosarcoma cells.
31926225	4	57	theme	Higher	450:455	arg1	behavior					466:473	Higher swelling behavior	450:473	Higher swelling behavior	450:473	Higher swelling behavior was observed in simulated intestinal fluid (SIF) with increasing GT content in SA formulations.
31926225	6	58	theme	weight	771:776	arg1	biodegradation					745:758	increased biodegradation	735:758	increased biodegradation (up to 82% weight loss)	735:782	GT incorporated hydrogel beads exhibited increased biodegradation (up to 82% weight loss) in biodegradation media (in vitro).
31926225	6	58	theme	weight	771:776	arg1	loss					778:781	up to 82% weight loss	761:781	up to 82% weight loss	761:781	GT incorporated hydrogel beads exhibited increased biodegradation (up to 82% weight loss) in biodegradation media (in vitro).
31926225	2	59	theme	phenolic	309:316	arg1	compounds					318:326	phenolic compounds	309:326	phenolic compounds extracted from Basella sps	309:353	The composite hydrogel beads were encapsulated with phenolic compounds extracted from Basella sps.
31926225	6	60	dep	%	769:769	arg1	82					767:768	82	767:768	82	767:768	GT incorporated hydrogel beads exhibited increased biodegradation (up to 82% weight loss) in biodegradation media (in vitro).
31926225	9	61	theme	remarkable	1017:1026	arg1	cytotoxicity					1028:1039	remarkable cytotoxicity	1017:1039	remarkable cytotoxicity against osteosarcoma cells	1017:1066	Furthermore, SA-GT encapsulated hydrogels showed remarkable cytotoxicity against osteosarcoma cells.
31926225	0	62	theme	gum	69:71	arg1	tragacanth					73:82	gum tragacanth	69:82	gum tragacanth for intestinal targeting of nutraceuticals	69:125	Novel pH-sensitive alginate hydrogel delivery system reinforced with gum tragacanth for intestinal targeting of nutraceuticals.
31926225	9	63	theme	encapsulated	987:998	arg1	hydrogels					1000:1008	SA-GT encapsulated hydrogels	981:1008	SA-GT encapsulated hydrogels	981:1008	Furthermore, SA-GT encapsulated hydrogels showed remarkable cytotoxicity against osteosarcoma cells.
31926225	1	64	theme	novel	159:163	arg1	combination					165:175	the novel combination	155:175	the novel combination of Gum tragacanth (GT) and sodium alginate (SA)	155:223	The present study utilizes the novel combination of Gum tragacanth (GT) and sodium alginate (SA) to reinforce SA hydrogel beads.
31926225	5	65	theme	compounds	676:684	arg1	release					656:662	sustained release	646:662	sustained release of phenolic compounds in SIF	646:691	SA-GT composite hydrogels revealed increased encapsulation efficiency with sustained release of phenolic compounds in SIF.
34717089	6	0	theme	films	1006:1010	arg1	activity					980:987	antioxidant activity	968:987	antioxidant activity of the composite films	968:1010	Moreover, the incorporated of the two modified silica nanoparticles significantly increased antioxidant activity of the composite films.
34717089	7	1	theme	antioxidant	1171:1181	arg1	films					1208:1212	novel antioxidant food packaging composite films	1165:1212	novel antioxidant food packaging composite films	1165:1212	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	7	1	theme	antioxidant	1171:1181	arg1	films					1049:1053	composite films	1039:1053	composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs	1039:1148	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	1	2	theme	gallic	251:256	arg1	acid					258:261	gallic acid	251:261	gallic acid onto silica nanoparticles	251:287	In this study, two modified silica nanoparticles (SiO2-GA NPs) were successfully obtained by covalently grafting gallic acid onto silica nanoparticles.
34717089	0	3	theme	silica	88:93	arg1	nanoparticles					95:107	modified silica nanoparticles	79:107	modified silica nanoparticles as an active food packaging	79:135	Characterization and antioxidant properties of chitosan film incorporated with modified silica nanoparticles as an active food packaging.
34717089	7	4	theme	food	1183:1186	arg1	films					1208:1212	novel antioxidant food packaging composite films	1165:1212	novel antioxidant food packaging composite films	1165:1212	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	7	4	theme	food	1183:1186	arg1	films					1049:1053	composite films	1039:1053	composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs	1039:1148	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	3	5	theme	4-SiO2-GA	517:525	arg1	NPs					527:529	4-SiO2-GA NPs	517:529	4-SiO2-GA NPs	517:529	Novel antioxidant active packaging composite films were prepared by incorporation of 1-SiO2-GA NPs or 4-SiO2-GA NPs into chitosan.
34717089	4	6	theme	films	586:590	arg1	analysis					560:567	The structure analysis	546:567	The structure analysis of the composite films	546:590	The structure analysis of the composite films showed that intermolecular hydrogen bonds were formed between the two modified silica nanoparticles and chitosan.
34717089	5	7	theme	mechanical	743:752	arg1	properties					754:763	the mechanical properties	739:763	the mechanical properties	739:763	Compared with the chitosan film, the mechanical properties, water vapor barrier property and UV light barrier ability of the composite films were significantly improved.
34717089	6	8	theme	silica	923:928	arg1	nanoparticles					930:942	the two modified silica nanoparticles	906:942	the two modified silica nanoparticles	906:942	Moreover, the incorporated of the two modified silica nanoparticles significantly increased antioxidant activity of the composite films.
34717089	2	9	theme	mean	294:297	arg1	diameters					308:316	The mean particle diameters	290:316	The mean particle diameters of their	290:325	The mean particle diameters of their were 112.7 ± 0.55 nm (1-SiO2-GA NPs) and 408.7 ± 3.20 nm (4-SiO2-GA NPs), respectively.
34717089	2	9	theme	mean	294:297	arg1	112.7 ± 0.55 nm					332:346	112.7 ± 0.55 nm	332:346	112.7 ± 0.55 nm	332:346	The mean particle diameters of their were 112.7 ± 0.55 nm (1-SiO2-GA NPs) and 408.7 ± 3.20 nm (4-SiO2-GA NPs), respectively.
34717089	2	10	theme	particle	299:306	arg1	diameters					308:316	The mean particle diameters	290:316	The mean particle diameters of their	290:325	The mean particle diameters of their were 112.7 ± 0.55 nm (1-SiO2-GA NPs) and 408.7 ± 3.20 nm (4-SiO2-GA NPs), respectively.
34717089	2	10	theme	particle	299:306	arg1	112.7 ± 0.55 nm					332:346	112.7 ± 0.55 nm	332:346	112.7 ± 0.55 nm	332:346	The mean particle diameters of their were 112.7 ± 0.55 nm (1-SiO2-GA NPs) and 408.7 ± 3.20 nm (4-SiO2-GA NPs), respectively.
34717089	4	11	theme	intermolecular	604:617	arg1	bonds					628:632	intermolecular hydrogen bonds	604:632	intermolecular hydrogen bonds	604:632	The structure analysis of the composite films showed that intermolecular hydrogen bonds were formed between the two modified silica nanoparticles and chitosan.
34717089	6	12	theme	modified	914:921	arg1	nanoparticles					930:942	the two modified silica nanoparticles	906:942	the two modified silica nanoparticles	906:942	Moreover, the incorporated of the two modified silica nanoparticles significantly increased antioxidant activity of the composite films.
34717089	7	13	theme	novel	1165:1169	arg1	films					1208:1212	novel antioxidant food packaging composite films	1165:1212	novel antioxidant food packaging composite films	1165:1212	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	7	13	theme	novel	1165:1169	arg1	films					1049:1053	composite films	1039:1053	composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs	1039:1148	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	1	14	theme	silica	268:273	arg1	nanoparticles					275:287	silica nanoparticles	268:287	silica nanoparticles	268:287	In this study, two modified silica nanoparticles (SiO2-GA NPs) were successfully obtained by covalently grafting gallic acid onto silica nanoparticles.
34717089	0	15	theme	food	122:125	arg1	packaging					127:135	an active food packaging	112:135	an active food packaging	112:135	Characterization and antioxidant properties of chitosan film incorporated with modified silica nanoparticles as an active food packaging.
34717089	3	16	theme	1-SiO2-GA	500:508	arg1	NPs					510:512	1-SiO2-GA NPs	500:512	1-SiO2-GA NPs	500:512	Novel antioxidant active packaging composite films were prepared by incorporation of 1-SiO2-GA NPs or 4-SiO2-GA NPs into chitosan.
34717089	3	17	theme	antioxidant	421:431	arg1	films					460:464	Novel antioxidant active packaging composite films	415:464	Novel antioxidant active packaging composite films	415:464	Novel antioxidant active packaging composite films were prepared by incorporation of 1-SiO2-GA NPs or 4-SiO2-GA NPs into chitosan.
34717089	1	18	theme	silica	166:171	arg1	nanoparticles					173:185	two modified silica nanoparticles	153:185	two modified silica nanoparticles (SiO2-GA NPs)	153:199	In this study, two modified silica nanoparticles (SiO2-GA NPs) were successfully obtained by covalently grafting gallic acid onto silica nanoparticles.
34717089	1	18	theme	silica	166:171	arg1	NPs					196:198	SiO2-GA NPs	188:198	SiO2-GA NPs	188:198	In this study, two modified silica nanoparticles (SiO2-GA NPs) were successfully obtained by covalently grafting gallic acid onto silica nanoparticles.
34717089	0	19	theme	active	115:120	arg1	packaging					127:135	an active food packaging	112:135	an active food packaging	112:135	Characterization and antioxidant properties of chitosan film incorporated with modified silica nanoparticles as an active food packaging.
34717089	5	20	theme	UV	799:800	arg1	light					802:806	UV light	799:806	UV light barrier ability	799:822	Compared with the chitosan film, the mechanical properties, water vapor barrier property and UV light barrier ability of the composite films were significantly improved.
34717089	7	21	theme	packaging	1188:1196	arg1	films					1208:1212	novel antioxidant food packaging composite films	1165:1212	novel antioxidant food packaging composite films	1165:1212	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	7	21	theme	packaging	1188:1196	arg1	films					1049:1053	composite films	1039:1053	composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs	1039:1148	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	3	22	theme	composite	450:458	arg1	films					460:464	Novel antioxidant active packaging composite films	415:464	Novel antioxidant active packaging composite films	415:464	Novel antioxidant active packaging composite films were prepared by incorporation of 1-SiO2-GA NPs or 4-SiO2-GA NPs into chitosan.
34717089	7	23	theme	NPs	1146:1148	arg1	incorporation					1119:1131	the incorporation	1115:1131	the incorporation of 1-SiO2-GA NPs	1115:1148	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	7	23	theme	NPs	1146:1148	arg1	nanoparticles					1089:1101	modified silica nanoparticles	1073:1101	modified silica nanoparticles	1073:1101	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	5	24	theme	vapor	772:776	arg1	property					786:793	water vapor barrier property	766:793	water vapor barrier property	766:793	Compared with the chitosan film, the mechanical properties, water vapor barrier property and UV light barrier ability of the composite films were significantly improved.
34717089	2	25	theme	their	321:325	arg1	diameters					308:316	The mean particle diameters	290:316	The mean particle diameters of their	290:325	The mean particle diameters of their were 112.7 ± 0.55 nm (1-SiO2-GA NPs) and 408.7 ± 3.20 nm (4-SiO2-GA NPs), respectively.
34717089	2	25	theme	their	321:325	arg1	112.7 ± 0.55 nm					332:346	112.7 ± 0.55 nm	332:346	112.7 ± 0.55 nm	332:346	The mean particle diameters of their were 112.7 ± 0.55 nm (1-SiO2-GA NPs) and 408.7 ± 3.20 nm (4-SiO2-GA NPs), respectively.
34717089	2	26	theme	4-SiO2-GA	385:393	arg1	NPs					395:397	4-SiO2-GA NPs	385:397	4-SiO2-GA NPs	385:397	The mean particle diameters of their were 112.7 ± 0.55 nm (1-SiO2-GA NPs) and 408.7 ± 3.20 nm (4-SiO2-GA NPs), respectively.
34717089	2	26	theme	4-SiO2-GA	385:393	arg1	408.7 ± 3.20 nm					368:382	408.7 ± 3.20 nm	368:382	408.7 ± 3.20 nm	368:382	The mean particle diameters of their were 112.7 ± 0.55 nm (1-SiO2-GA NPs) and 408.7 ± 3.20 nm (4-SiO2-GA NPs), respectively.
34717089	1	27	theme	SiO2-GA	188:194	arg1	nanoparticles					173:185	two modified silica nanoparticles	153:185	two modified silica nanoparticles (SiO2-GA NPs)	153:199	In this study, two modified silica nanoparticles (SiO2-GA NPs) were successfully obtained by covalently grafting gallic acid onto silica nanoparticles.
34717089	1	27	theme	SiO2-GA	188:194	arg1	NPs					196:198	SiO2-GA NPs	188:198	SiO2-GA NPs	188:198	In this study, two modified silica nanoparticles (SiO2-GA NPs) were successfully obtained by covalently grafting gallic acid onto silica nanoparticles.
34717089	5	28	theme	composite	831:839	arg1	films					841:845	the composite films	827:845	the composite films	827:845	Compared with the chitosan film, the mechanical properties, water vapor barrier property and UV light barrier ability of the composite films were significantly improved.
34717089	5	29	theme	chitosan	724:731	arg1	film					733:736	the chitosan film	720:736	the chitosan film	720:736	Compared with the chitosan film, the mechanical properties, water vapor barrier property and UV light barrier ability of the composite films were significantly improved.
34717089	0	30	theme	antioxidant	21:31	arg1	properties					33:42	antioxidant properties	21:42	antioxidant properties	21:42	Characterization and antioxidant properties of chitosan film incorporated with modified silica nanoparticles as an active food packaging.
34717089	4	31	theme	composite	576:584	arg1	films					586:590	the composite films	572:590	the composite films	572:590	The structure analysis of the composite films showed that intermolecular hydrogen bonds were formed between the two modified silica nanoparticles and chitosan.
34717089	7	32	theme	1-SiO2-GA	1136:1144	arg1	NPs					1146:1148	1-SiO2-GA NPs	1136:1148	1-SiO2-GA NPs	1136:1148	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	1	33	theme	modified	157:164	arg1	nanoparticles					173:185	two modified silica nanoparticles	153:185	two modified silica nanoparticles (SiO2-GA NPs)	153:199	In this study, two modified silica nanoparticles (SiO2-GA NPs) were successfully obtained by covalently grafting gallic acid onto silica nanoparticles.
34717089	1	33	theme	modified	157:164	arg1	NPs					196:198	SiO2-GA NPs	188:198	SiO2-GA NPs	188:198	In this study, two modified silica nanoparticles (SiO2-GA NPs) were successfully obtained by covalently grafting gallic acid onto silica nanoparticles.
34717089	0	34	theme	chitosan	47:54	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and antioxidant properties of chitosan film incorporated with modified silica nanoparticles as an active food packaging.
34717089	0	34	theme	chitosan	47:54	arg1	properties					33:42	antioxidant properties	21:42	antioxidant properties	21:42	Characterization and antioxidant properties of chitosan film incorporated with modified silica nanoparticles as an active food packaging.
34717089	6	35	theme	composite	996:1004	arg1	films					1006:1010	the composite films	992:1010	the composite films	992:1010	Moreover, the incorporated of the two modified silica nanoparticles significantly increased antioxidant activity of the composite films.
34717089	3	36	theme	active	433:438	arg1	films					460:464	Novel antioxidant active packaging composite films	415:464	Novel antioxidant active packaging composite films	415:464	Novel antioxidant active packaging composite films were prepared by incorporation of 1-SiO2-GA NPs or 4-SiO2-GA NPs into chitosan.
34717089	4	37	theme	modified	662:669	arg1	nanoparticles					678:690	the two modified silica nanoparticles	654:690	the two modified silica nanoparticles	654:690	The structure analysis of the composite films showed that intermolecular hydrogen bonds were formed between the two modified silica nanoparticles and chitosan.
34717089	3	38	theme	packaging	440:448	arg1	films					460:464	Novel antioxidant active packaging composite films	415:464	Novel antioxidant active packaging composite films	415:464	Novel antioxidant active packaging composite films were prepared by incorporation of 1-SiO2-GA NPs or 4-SiO2-GA NPs into chitosan.
34717089	3	39	theme	Novel	415:419	arg1	films					460:464	Novel antioxidant active packaging composite films	415:464	Novel antioxidant active packaging composite films	415:464	Novel antioxidant active packaging composite films were prepared by incorporation of 1-SiO2-GA NPs or 4-SiO2-GA NPs into chitosan.
34717089	5	40	theme	water	766:770	arg1	property					786:793	water vapor barrier property	766:793	water vapor barrier property	766:793	Compared with the chitosan film, the mechanical properties, water vapor barrier property and UV light barrier ability of the composite films were significantly improved.
34717089	5	41	theme	barrier	808:814	arg1	ability					816:822	UV light barrier ability	799:822	UV light barrier ability	799:822	Compared with the chitosan film, the mechanical properties, water vapor barrier property and UV light barrier ability of the composite films were significantly improved.
34717089	7	42	theme	modified	1073:1080	arg1	incorporation					1119:1131	the incorporation	1115:1131	the incorporation of 1-SiO2-GA NPs	1115:1148	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	7	42	theme	modified	1073:1080	arg1	nanoparticles					1089:1101	modified silica nanoparticles	1073:1101	modified silica nanoparticles	1073:1101	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	2	43	theme	1-SiO2-GA	349:357	arg1	112.7 ± 0.55 nm					332:346	112.7 ± 0.55 nm	332:346	112.7 ± 0.55 nm	332:346	The mean particle diameters of their were 112.7 ± 0.55 nm (1-SiO2-GA NPs) and 408.7 ± 3.20 nm (4-SiO2-GA NPs), respectively.
34717089	2	43	theme	1-SiO2-GA	349:357	arg1	NPs					359:361	1-SiO2-GA NPs	349:361	1-SiO2-GA NPs	349:361	The mean particle diameters of their were 112.7 ± 0.55 nm (1-SiO2-GA NPs) and 408.7 ± 3.20 nm (4-SiO2-GA NPs), respectively.
34717089	7	44	theme	composite	1198:1206	arg1	films					1208:1212	novel antioxidant food packaging composite films	1165:1212	novel antioxidant food packaging composite films	1165:1212	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	7	44	theme	composite	1198:1206	arg1	films					1049:1053	composite films	1039:1053	composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs	1039:1148	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	4	45	theme	structure	550:558	arg1	analysis					560:567	The structure analysis	546:567	The structure analysis of the composite films	546:590	The structure analysis of the composite films showed that intermolecular hydrogen bonds were formed between the two modified silica nanoparticles and chitosan.
34717089	7	46	used	used	1157:1160	arg2	films					1049:1053	composite films	1039:1053	composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs	1039:1148	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	7	46	used	used	1157:1160	arg2	films					1208:1212	novel antioxidant food packaging composite films	1165:1212	novel antioxidant food packaging composite films	1165:1212	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	3	47	theme	NPs	510:512	arg1	incorporation					483:495	incorporation	483:495	incorporation of 1-SiO2-GA NPs or 4-SiO2-GA NPs into chitosan	483:543	Novel antioxidant active packaging composite films were prepared by incorporation of 1-SiO2-GA NPs or 4-SiO2-GA NPs into chitosan.
34717089	6	48	theme	antioxidant	968:978	arg1	activity					980:987	antioxidant activity	968:987	antioxidant activity of the composite films	968:1010	Moreover, the incorporated of the two modified silica nanoparticles significantly increased antioxidant activity of the composite films.
34717089	7	49	theme	silica	1082:1087	arg1	incorporation					1119:1131	the incorporation	1115:1131	the incorporation of 1-SiO2-GA NPs	1115:1148	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	7	49	theme	silica	1082:1087	arg1	nanoparticles					1089:1101	modified silica nanoparticles	1073:1101	modified silica nanoparticles	1073:1101	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	5	50	theme	films	841:845	arg1	ability					816:822	UV light barrier ability	799:822	UV light barrier ability	799:822	Compared with the chitosan film, the mechanical properties, water vapor barrier property and UV light barrier ability of the composite films were significantly improved.
34717089	5	50	theme	films	841:845	arg1	property					786:793	water vapor barrier property	766:793	water vapor barrier property	766:793	Compared with the chitosan film, the mechanical properties, water vapor barrier property and UV light barrier ability of the composite films were significantly improved.
34717089	5	50	theme	films	841:845	arg1	properties					754:763	the mechanical properties	739:763	the mechanical properties	739:763	Compared with the chitosan film, the mechanical properties, water vapor barrier property and UV light barrier ability of the composite films were significantly improved.
34717089	4	51	theme	hydrogen	619:626	arg1	bonds					628:632	intermolecular hydrogen bonds	604:632	intermolecular hydrogen bonds	604:632	The structure analysis of the composite films showed that intermolecular hydrogen bonds were formed between the two modified silica nanoparticles and chitosan.
34717089	0	52	theme	modified	79:86	arg1	nanoparticles					95:107	modified silica nanoparticles	79:107	modified silica nanoparticles as an active food packaging	79:135	Characterization and antioxidant properties of chitosan film incorporated with modified silica nanoparticles as an active food packaging.
34717089	7	53	theme	composite	1039:1047	arg1	films					1208:1212	novel antioxidant food packaging composite films	1165:1212	novel antioxidant food packaging composite films	1165:1212	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	7	53	theme	composite	1039:1047	arg1	films					1049:1053	composite films	1039:1053	composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs	1039:1148	This study indicates that composite films incorporated with modified silica nanoparticles, especially the incorporation of 1-SiO2-GA NPs can be used as novel antioxidant food packaging composite films.
34717089	3	54	theme	NPs	527:529	arg1	incorporation					483:495	incorporation	483:495	incorporation of 1-SiO2-GA NPs or 4-SiO2-GA NPs into chitosan	483:543	Novel antioxidant active packaging composite films were prepared by incorporation of 1-SiO2-GA NPs or 4-SiO2-GA NPs into chitosan.
34717089	4	55	theme	silica	671:676	arg1	nanoparticles					678:690	the two modified silica nanoparticles	654:690	the two modified silica nanoparticles	654:690	The structure analysis of the composite films showed that intermolecular hydrogen bonds were formed between the two modified silica nanoparticles and chitosan.
34717089	5	56	theme	barrier	778:784	arg1	property					786:793	water vapor barrier property	766:793	water vapor barrier property	766:793	Compared with the chitosan film, the mechanical properties, water vapor barrier property and UV light barrier ability of the composite films were significantly improved.
34717089	5	57	theme	light	802:806	arg1	ability					816:822	UV light barrier ability	799:822	UV light barrier ability	799:822	Compared with the chitosan film, the mechanical properties, water vapor barrier property and UV light barrier ability of the composite films were significantly improved.
32355733	12	0	theme	MGAT4A	1978:1983	arg1	expression					1952:1961	gene expression	1947:1961	gene expression of ST3GAL6 and MGAT4A	1947:1983	Transcriptome sequencing and RT-PCR showed that gene expression of ST3GAL6 and MGAT4A increased, while gene expression of FUT11, FUT1, GMDS, and B4GALT5 decreased in three cisplatin-resistant variants.
32355733	2	1	gly	glycosylation	323:335	arg1	resistance					367:376	platinum resistance	358:376	platinum resistance	358:376	Abnormal glycosylation has been reported in platinum resistance.
32355733	5	2	theme	resistance-related	705:722	arg1	N-glycans					724:732	platinum resistance-related N-glycans	696:732	platinum resistance-related N-glycans in ovarian cancer cells	696:756	Therefore, the objective of this study was to determine platinum resistance-related N-glycans in ovarian cancer cells during continuous exposure to cisplatin.
32355733	9	3	theme	N-glycomic	1264:1273	arg1	changes					1275:1281	dynamic N-glycomic changes	1256:1281	dynamic N-glycomic changes during the development of platinum resistance in cisplatin-resistant variants	1256:1359	Analysis of dynamic N-glycomic changes during the development of platinum resistance in cisplatin-resistant variants was performed with MALDI-time-of-flight (TOF)-MS combined with ethyl esterification derivatization, which were used to discriminate between α2,3- and α2,6-linkage N-acetylneuraminic acid.
32355733	9	4	from	variants	1352:1359	arg1	development					1294:1304	the development	1290:1304	the development of platinum resistance in cisplatin-resistant variants	1290:1359	Analysis of dynamic N-glycomic changes during the development of platinum resistance in cisplatin-resistant variants was performed with MALDI-time-of-flight (TOF)-MS combined with ethyl esterification derivatization, which were used to discriminate between α2,3- and α2,6-linkage N-acetylneuraminic acid.
32355733	11	5	theme	fucosylated	1829:1839	arg1	glycans					1841:1847	fucosylated glycans	1829:1847	fucosylated glycans	1829:1847	RESULTS Compared to the A2780 cells, MS analysis indicated that α2,3-linked sialic structures and N-glycan gal-ratios were significantly higher, while fucosylated glycans were lower in three cisplatin-resistant variants.
32355733	6	6	theme	resistance	860:869	arg1	mechanism					838:846	the mechanism	834:846	the mechanism of platinum resistance	834:869	These glycans might be involved in the mechanism of platinum resistance and serve as biomarkers to monitor its development.
32355733	9	7	theme	platinum	1309:1316	arg1	resistance					1318:1327	platinum resistance	1309:1327	platinum resistance in cisplatin-resistant variants	1309:1359	Analysis of dynamic N-glycomic changes during the development of platinum resistance in cisplatin-resistant variants was performed with MALDI-time-of-flight (TOF)-MS combined with ethyl esterification derivatization, which were used to discriminate between α2,3- and α2,6-linkage N-acetylneuraminic acid.
32355733	12	8	theme	gene	1947:1950	arg1	expression					1952:1961	gene expression	1947:1961	gene expression of ST3GAL6 and MGAT4A	1947:1983	Transcriptome sequencing and RT-PCR showed that gene expression of ST3GAL6 and MGAT4A increased, while gene expression of FUT11, FUT1, GMDS, and B4GALT5 decreased in three cisplatin-resistant variants.
32355733	1	9	from	mortality	285:293	arg1	cancer					306:311	ovarian cancer	298:311	ovarian cancer	298:311	BACKGROUND Platinum resistance development is a dynamic process that occurs during continuous chemotherapy and contributes to high mortality in ovarian cancer.
32355733	13	10	theme	ovarian	2319:2325	arg1	patients					2334:2341	ovarian cancer patients	2319:2341	ovarian cancer patients	2319:2341	CONCLUSIONS Analysis of N-glycans and glycogene expression showed that α2,3-linked sialic structures might serve as biomarkers to monitor the development of platinum resistance and to guide individualized treatment of ovarian cancer patients.
32355733	2	11	theme	Abnormal	314:321	arg1	glycosylation					323:335	Abnormal glycosylation	314:335	Abnormal glycosylation	314:335	Abnormal glycosylation has been reported in platinum resistance.
32355733	9	12	used	used	1472:1475	arg2	derivatization					1445:1458	ethyl esterification derivatization	1424:1458	ethyl esterification derivatization	1424:1458	Analysis of dynamic N-glycomic changes during the development of platinum resistance in cisplatin-resistant variants was performed with MALDI-time-of-flight (TOF)-MS combined with ethyl esterification derivatization, which were used to discriminate between α2,3- and α2,6-linkage N-acetylneuraminic acid.
32355733	3	13	from	studies	384:390	arg1	resistance					404:413	platinum resistance	395:413	platinum resistance	395:413	Many studies on platinum resistance have been performed, but few of them have investigated platinum resistance-associated glycans based on N-glycomics.
32355733	5	14	theme	cancer	745:750	arg1	cells					752:756	ovarian cancer cells	737:756	ovarian cancer cells	737:756	Therefore, the objective of this study was to determine platinum resistance-related N-glycans in ovarian cancer cells during continuous exposure to cisplatin.
32355733	13	15	theme	expression	2149:2158	arg1	Analysis					2113:2120	CONCLUSIONS Analysis	2101:2120	CONCLUSIONS Analysis of N-glycans and glycogene expression	2101:2158	CONCLUSIONS Analysis of N-glycans and glycogene expression showed that α2,3-linked sialic structures might serve as biomarkers to monitor the development of platinum resistance and to guide individualized treatment of ovarian cancer patients.
32355733	9	16	from	development	1294:1304	arg1	variants					1352:1359	cisplatin-resistant variants	1332:1359	cisplatin-resistant variants	1332:1359	Analysis of dynamic N-glycomic changes during the development of platinum resistance in cisplatin-resistant variants was performed with MALDI-time-of-flight (TOF)-MS combined with ethyl esterification derivatization, which were used to discriminate between α2,3- and α2,6-linkage N-acetylneuraminic acid.
32355733	9	17	theme	MALDI-time-of-flight	1380:1399	arg1	-MS					1406:1408	MALDI-time-of-flight (TOF)-MS	1380:1408	MALDI-time-of-flight (TOF)-MS combined with ethyl esterification derivatization, which were used to discriminate between α2,3- and α2,6-linkage N-acetylneuraminic acid	1380:1546	Analysis of dynamic N-glycomic changes during the development of platinum resistance in cisplatin-resistant variants was performed with MALDI-time-of-flight (TOF)-MS combined with ethyl esterification derivatization, which were used to discriminate between α2,3- and α2,6-linkage N-acetylneuraminic acid.
32355733	12	18	theme	Transcriptome	1899:1911	arg1	sequencing					1913:1922	Transcriptome sequencing	1899:1922	Transcriptome sequencing	1899:1922	Transcriptome sequencing and RT-PCR showed that gene expression of ST3GAL6 and MGAT4A increased, while gene expression of FUT11, FUT1, GMDS, and B4GALT5 decreased in three cisplatin-resistant variants.
32355733	13	19	theme	CONCLUSIONS	2101:2111	arg1	Analysis					2113:2120	CONCLUSIONS Analysis	2101:2120	CONCLUSIONS Analysis of N-glycans and glycogene expression	2101:2158	CONCLUSIONS Analysis of N-glycans and glycogene expression showed that α2,3-linked sialic structures might serve as biomarkers to monitor the development of platinum resistance and to guide individualized treatment of ovarian cancer patients.
32355733	13	20	theme	platinum	2258:2265	arg1	resistance					2267:2276	platinum resistance	2258:2276	platinum resistance	2258:2276	CONCLUSIONS Analysis of N-glycans and glycogene expression showed that α2,3-linked sialic structures might serve as biomarkers to monitor the development of platinum resistance and to guide individualized treatment of ovarian cancer patients.
32355733	13	21	theme	cancer	2327:2332	arg1	patients					2334:2341	ovarian cancer patients	2319:2341	ovarian cancer patients	2319:2341	CONCLUSIONS Analysis of N-glycans and glycogene expression showed that α2,3-linked sialic structures might serve as biomarkers to monitor the development of platinum resistance and to guide individualized treatment of ovarian cancer patients.
32355733	8	22	theme	maximal	1118:1124	arg1	values					1158:1163	half maximal inhibitory concentration (IC50) values	1113:1163	half maximal inhibitory concentration (IC50) values	1113:1163	Cisplatin-resistant variants were confirmed by higher half maximal inhibitory concentration (IC50) values and increased P-glycoprotein (ABCB1, P-gp) expression compared to A2780 cells.
32355733	1	23	theme	Platinum	165:172	arg1	process					210:216	a dynamic process	200:216	a dynamic process that occurs during continuous chemotherapy and contributes to high mortality in ovarian cancer	200:311	BACKGROUND Platinum resistance development is a dynamic process that occurs during continuous chemotherapy and contributes to high mortality in ovarian cancer.
32355733	1	23	theme	Platinum	165:172	arg1	development					185:195	BACKGROUND Platinum resistance development	154:195	BACKGROUND Platinum resistance development	154:195	BACKGROUND Platinum resistance development is a dynamic process that occurs during continuous chemotherapy and contributes to high mortality in ovarian cancer.
32355733	0	24	theme	cancer	88:93	arg1	line					100:103	ovarian cancer cell line A2780	80:109	ovarian cancer cell line A2780	80:109	Dynamic analysis of N-glycomic and transcriptomic changes in the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants.
32355733	8	25	theme	concentration	1137:1149	arg1	values					1158:1163	half maximal inhibitory concentration (IC50) values	1113:1163	half maximal inhibitory concentration (IC50) values	1113:1163	Cisplatin-resistant variants were confirmed by higher half maximal inhibitory concentration (IC50) values and increased P-glycoprotein (ABCB1, P-gp) expression compared to A2780 cells.
32355733	0	26	from	changes	50:56	arg1	development					65:75	the development	61:75	the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants	61:151	Dynamic analysis of N-glycomic and transcriptomic changes in the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants.
32355733	10	27	theme	transcriptome	1625:1637	arg1	sequencing					1639:1648	transcriptome sequencing	1625:1648	transcriptome sequencing	1625:1648	N-glycan alterations were further validated on a glycotransferase level via transcriptome sequencing and real-time PCR (RT-PCR).
32355733	7	28	from	development	955:965	arg1	cancer					1001:1006	ovarian cancer	993:1006	ovarian cancer	993:1006	METHODS This study mimicked the development of platinum resistance in ovarian cancer by continuously exposing A2780 cells to cisplatin.
32355733	8	29	gly	P-glycoprotein	1179:1192	arg1	P-glycoprotein					1179:1192	P-glycoprotein	1179:1192	increased P-glycoprotein (ABCB1, P-gp) expression	1169:1217	Cisplatin-resistant variants were confirmed by higher half maximal inhibitory concentration (IC50) values and increased P-glycoprotein (ABCB1, P-gp) expression compared to A2780 cells.
32355733	0	30	theme	line	100:103	arg1	development					65:75	the development	61:75	the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants	61:151	Dynamic analysis of N-glycomic and transcriptomic changes in the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants.
32355733	9	31	theme	esterification	1430:1443	arg1	derivatization					1445:1458	ethyl esterification derivatization	1424:1458	ethyl esterification derivatization	1424:1458	Analysis of dynamic N-glycomic changes during the development of platinum resistance in cisplatin-resistant variants was performed with MALDI-time-of-flight (TOF)-MS combined with ethyl esterification derivatization, which were used to discriminate between α2,3- and α2,6-linkage N-acetylneuraminic acid.
32355733	8	32	theme	IC50	1152:1155	arg1	values					1158:1163	half maximal inhibitory concentration (IC50) values	1113:1163	half maximal inhibitory concentration (IC50) values	1113:1163	Cisplatin-resistant variants were confirmed by higher half maximal inhibitory concentration (IC50) values and increased P-glycoprotein (ABCB1, P-gp) expression compared to A2780 cells.
32355733	13	33	theme	N-glycans	2125:2133	arg1	Analysis					2113:2120	CONCLUSIONS Analysis	2101:2120	CONCLUSIONS Analysis of N-glycans and glycogene expression	2101:2158	CONCLUSIONS Analysis of N-glycans and glycogene expression showed that α2,3-linked sialic structures might serve as biomarkers to monitor the development of platinum resistance and to guide individualized treatment of ovarian cancer patients.
32355733	0	34	theme	Dynamic	0:6	arg1	analysis					8:15	Dynamic analysis	0:15	Dynamic analysis of N-glycomic and transcriptomic changes in the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants.	0:152	Dynamic analysis of N-glycomic and transcriptomic changes in the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants.
32355733	3	35	theme	platinum	395:402	arg1	resistance					404:413	platinum resistance	395:413	platinum resistance	395:413	Many studies on platinum resistance have been performed, but few of them have investigated platinum resistance-associated glycans based on N-glycomics.
32355733	4	36	theme	ovarian	604:610	arg1	cancer					612:617	ovarian cancer	604:617	ovarian cancer	604:617	Moreover, glycomic alterations during platinum resistance development in ovarian cancer are rarely reported.
32355733	13	37	link	α2,3-linked	2172:2182	arg1	structures					2191:2200	α2,3-linked sialic structures	2172:2200	α2,3-linked sialic structures	2172:2200	CONCLUSIONS Analysis of N-glycans and glycogene expression showed that α2,3-linked sialic structures might serve as biomarkers to monitor the development of platinum resistance and to guide individualized treatment of ovarian cancer patients.
32355733	13	37	link	α2,3-linked	2172:2182	arg1	biomarkers					2217:2226	biomarkers	2217:2226	biomarkers	2217:2226	CONCLUSIONS Analysis of N-glycans and glycogene expression showed that α2,3-linked sialic structures might serve as biomarkers to monitor the development of platinum resistance and to guide individualized treatment of ovarian cancer patients.
32355733	8	38	theme	increased	1169:1177	arg1	expression					1208:1217	increased P-glycoprotein (ABCB1, P-gp) expression	1169:1217	increased P-glycoprotein (ABCB1, P-gp) expression	1169:1217	Cisplatin-resistant variants were confirmed by higher half maximal inhibitory concentration (IC50) values and increased P-glycoprotein (ABCB1, P-gp) expression compared to A2780 cells.
32355733	3	39	theme	platinum	470:477	arg1	glycans					501:507	platinum resistance-associated glycans	470:507	platinum resistance-associated glycans based on N-glycomics	470:528	Many studies on platinum resistance have been performed, but few of them have investigated platinum resistance-associated glycans based on N-glycomics.
32355733	4	40	from	development	589:599	arg1	cancer					612:617	ovarian cancer	604:617	ovarian cancer	604:617	Moreover, glycomic alterations during platinum resistance development in ovarian cancer are rarely reported.
32355733	5	41	from	N-glycans	724:732	arg1	cells					752:756	ovarian cancer cells	737:756	ovarian cancer cells	737:756	Therefore, the objective of this study was to determine platinum resistance-related N-glycans in ovarian cancer cells during continuous exposure to cisplatin.
32355733	4	42	theme	platinum	569:576	arg1	development					589:599	platinum resistance development	569:599	platinum resistance development in ovarian cancer	569:617	Moreover, glycomic alterations during platinum resistance development in ovarian cancer are rarely reported.
32355733	7	43	dep	METHODS	923:929	arg1	mimicked					942:949	mimicked	942:949	mimicked the development of platinum resistance in ovarian cancer by continuously exposing A2780 cells to cisplatin	942:1056	METHODS This study mimicked the development of platinum resistance in ovarian cancer by continuously exposing A2780 cells to cisplatin.
32355733	0	44	theme	changes	50:56	arg1	analysis					8:15	Dynamic analysis	0:15	Dynamic analysis of N-glycomic and transcriptomic changes in the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants.	0:152	Dynamic analysis of N-glycomic and transcriptomic changes in the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants.
32355733	7	45	theme	ovarian	993:999	arg1	cancer					1001:1006	ovarian cancer	993:1006	ovarian cancer	993:1006	METHODS This study mimicked the development of platinum resistance in ovarian cancer by continuously exposing A2780 cells to cisplatin.
32355733	12	46	theme	GMDS	2034:2037	arg1	expression					2007:2016	gene expression	2002:2016	gene expression of FUT11, FUT1, GMDS, and B4GALT5	2002:2050	Transcriptome sequencing and RT-PCR showed that gene expression of ST3GAL6 and MGAT4A increased, while gene expression of FUT11, FUT1, GMDS, and B4GALT5 decreased in three cisplatin-resistant variants.
32355733	11	47	theme	N-glycan	1776:1783	arg1	gal-ratios					1785:1794	N-glycan gal-ratios	1776:1794	N-glycan gal-ratios	1776:1794	RESULTS Compared to the A2780 cells, MS analysis indicated that α2,3-linked sialic structures and N-glycan gal-ratios were significantly higher, while fucosylated glycans were lower in three cisplatin-resistant variants.
32355733	7	48	theme	resistance	979:988	arg1	development					955:965	the development	951:965	the development of platinum resistance in ovarian cancer	951:1006	METHODS This study mimicked the development of platinum resistance in ovarian cancer by continuously exposing A2780 cells to cisplatin.
32355733	9	49	theme	N-acetylneuraminic	1524:1541	arg1	acid					1543:1546	α2,6-linkage N-acetylneuraminic acid	1511:1546	α2,6-linkage N-acetylneuraminic acid	1511:1546	Analysis of dynamic N-glycomic changes during the development of platinum resistance in cisplatin-resistant variants was performed with MALDI-time-of-flight (TOF)-MS combined with ethyl esterification derivatization, which were used to discriminate between α2,3- and α2,6-linkage N-acetylneuraminic acid.
32355733	5	50	theme	platinum	696:703	arg1	N-glycans					724:732	platinum resistance-related N-glycans	696:732	platinum resistance-related N-glycans in ovarian cancer cells	696:756	Therefore, the objective of this study was to determine platinum resistance-related N-glycans in ovarian cancer cells during continuous exposure to cisplatin.
32355733	9	51	theme	dynamic	1256:1262	arg1	changes					1275:1281	dynamic N-glycomic changes	1256:1281	dynamic N-glycomic changes during the development of platinum resistance in cisplatin-resistant variants	1256:1359	Analysis of dynamic N-glycomic changes during the development of platinum resistance in cisplatin-resistant variants was performed with MALDI-time-of-flight (TOF)-MS combined with ethyl esterification derivatization, which were used to discriminate between α2,3- and α2,6-linkage N-acetylneuraminic acid.
32355733	8	52	theme	Cisplatin-resistant	1059:1077	arg1	variants					1079:1086	Cisplatin-resistant variants	1059:1086	Cisplatin-resistant variants	1059:1086	Cisplatin-resistant variants were confirmed by higher half maximal inhibitory concentration (IC50) values and increased P-glycoprotein (ABCB1, P-gp) expression compared to A2780 cells.
32355733	6	53	theme	platinum	851:858	arg1	resistance					860:869	platinum resistance	851:869	platinum resistance	851:869	These glycans might be involved in the mechanism of platinum resistance and serve as biomarkers to monitor its development.
32355733	9	54	theme	changes	1275:1281	arg1	Analysis					1244:1251	Analysis	1244:1251	Analysis of dynamic N-glycomic changes during the development of platinum resistance in cisplatin-resistant variants	1244:1359	Analysis of dynamic N-glycomic changes during the development of platinum resistance in cisplatin-resistant variants was performed with MALDI-time-of-flight (TOF)-MS combined with ethyl esterification derivatization, which were used to discriminate between α2,3- and α2,6-linkage N-acetylneuraminic acid.
32355733	13	55	theme	glycogene	2139:2147	arg1	expression					2149:2158	glycogene expression	2139:2158	glycogene expression	2139:2158	CONCLUSIONS Analysis of N-glycans and glycogene expression showed that α2,3-linked sialic structures might serve as biomarkers to monitor the development of platinum resistance and to guide individualized treatment of ovarian cancer patients.
32355733	1	56	theme	high	280:283	arg1	mortality					285:293	high mortality	280:293	high mortality in ovarian cancer	280:311	BACKGROUND Platinum resistance development is a dynamic process that occurs during continuous chemotherapy and contributes to high mortality in ovarian cancer.
32355733	11	57	theme	α2,3-linked	1742:1752	arg1	structures					1761:1770	α2,3-linked sialic structures	1742:1770	α2,3-linked sialic structures	1742:1770	RESULTS Compared to the A2780 cells, MS analysis indicated that α2,3-linked sialic structures and N-glycan gal-ratios were significantly higher, while fucosylated glycans were lower in three cisplatin-resistant variants.
32355733	13	58	theme	patients	2334:2341	arg1	treatment					2306:2314	individualized treatment	2291:2314	individualized treatment of ovarian cancer patients	2291:2341	CONCLUSIONS Analysis of N-glycans and glycogene expression showed that α2,3-linked sialic structures might serve as biomarkers to monitor the development of platinum resistance and to guide individualized treatment of ovarian cancer patients.
32355733	5	59	theme	study	673:677	arg1	objective					655:663	the objective	651:663	the objective of this study	651:677	Therefore, the objective of this study was to determine platinum resistance-related N-glycans in ovarian cancer cells during continuous exposure to cisplatin.
32355733	1	60	theme	resistance	174:183	arg1	process					210:216	a dynamic process	200:216	a dynamic process that occurs during continuous chemotherapy and contributes to high mortality in ovarian cancer	200:311	BACKGROUND Platinum resistance development is a dynamic process that occurs during continuous chemotherapy and contributes to high mortality in ovarian cancer.
32355733	1	60	theme	resistance	174:183	arg1	development					185:195	BACKGROUND Platinum resistance development	154:195	BACKGROUND Platinum resistance development	154:195	BACKGROUND Platinum resistance development is a dynamic process that occurs during continuous chemotherapy and contributes to high mortality in ovarian cancer.
32355733	11	61	gly	fucosylated	1829:1839	arg1	glycans					1841:1847	fucosylated glycans	1829:1847	fucosylated glycans	1829:1847	RESULTS Compared to the A2780 cells, MS analysis indicated that α2,3-linked sialic structures and N-glycan gal-ratios were significantly higher, while fucosylated glycans were lower in three cisplatin-resistant variants.
32355733	11	62	theme	MS	1715:1716	arg1	analysis					1718:1725	MS analysis	1715:1725	MS analysis	1715:1725	RESULTS Compared to the A2780 cells, MS analysis indicated that α2,3-linked sialic structures and N-glycan gal-ratios were significantly higher, while fucosylated glycans were lower in three cisplatin-resistant variants.
32355733	8	63	theme	half	1113:1116	arg1	values					1158:1163	half maximal inhibitory concentration (IC50) values	1113:1163	half maximal inhibitory concentration (IC50) values	1113:1163	Cisplatin-resistant variants were confirmed by higher half maximal inhibitory concentration (IC50) values and increased P-glycoprotein (ABCB1, P-gp) expression compared to A2780 cells.
32355733	12	64	theme	cisplatin-resistant	2071:2089	arg1	variants					2091:2098	three cisplatin-resistant variants	2065:2098	three cisplatin-resistant variants	2065:2098	Transcriptome sequencing and RT-PCR showed that gene expression of ST3GAL6 and MGAT4A increased, while gene expression of FUT11, FUT1, GMDS, and B4GALT5 decreased in three cisplatin-resistant variants.
32355733	10	65	theme	N-glycan	1549:1556	arg1	alterations					1558:1568	N-glycan alterations	1549:1568	N-glycan alterations	1549:1568	N-glycan alterations were further validated on a glycotransferase level via transcriptome sequencing and real-time PCR (RT-PCR).
32355733	5	66	theme	continuous	765:774	arg1	exposure					776:783	continuous exposure	765:783	continuous exposure to cisplatin	765:796	Therefore, the objective of this study was to determine platinum resistance-related N-glycans in ovarian cancer cells during continuous exposure to cisplatin.
32355733	9	67	theme	resistance	1318:1327	arg1	development					1294:1304	the development	1290:1304	the development of platinum resistance in cisplatin-resistant variants	1290:1359	Analysis of dynamic N-glycomic changes during the development of platinum resistance in cisplatin-resistant variants was performed with MALDI-time-of-flight (TOF)-MS combined with ethyl esterification derivatization, which were used to discriminate between α2,3- and α2,6-linkage N-acetylneuraminic acid.
32355733	1	68	theme	dynamic	202:208	arg1	process					210:216	a dynamic process	200:216	a dynamic process that occurs during continuous chemotherapy and contributes to high mortality in ovarian cancer	200:311	BACKGROUND Platinum resistance development is a dynamic process that occurs during continuous chemotherapy and contributes to high mortality in ovarian cancer.
32355733	1	68	theme	dynamic	202:208	arg1	development					185:195	BACKGROUND Platinum resistance development	154:195	BACKGROUND Platinum resistance development	154:195	BACKGROUND Platinum resistance development is a dynamic process that occurs during continuous chemotherapy and contributes to high mortality in ovarian cancer.
32355733	0	69	theme	cisplatin-resistant	124:142	arg1	variants					144:151	its three cisplatin-resistant variants	114:151	its three cisplatin-resistant variants	114:151	Dynamic analysis of N-glycomic and transcriptomic changes in the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants.
32355733	9	70	theme	cisplatin-resistant	1332:1350	arg1	variants					1352:1359	cisplatin-resistant variants	1332:1359	cisplatin-resistant variants	1332:1359	Analysis of dynamic N-glycomic changes during the development of platinum resistance in cisplatin-resistant variants was performed with MALDI-time-of-flight (TOF)-MS combined with ethyl esterification derivatization, which were used to discriminate between α2,3- and α2,6-linkage N-acetylneuraminic acid.
32355733	0	71	from	development	65:75	arg1	analysis					8:15	Dynamic analysis	0:15	Dynamic analysis of N-glycomic and transcriptomic changes in the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants.	0:152	Dynamic analysis of N-glycomic and transcriptomic changes in the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants.
32355733	8	72	dep	P-glycoprotein	1179:1192	arg1	P-gp					1202:1205	P-gp	1202:1205	P-gp	1202:1205	Cisplatin-resistant variants were confirmed by higher half maximal inhibitory concentration (IC50) values and increased P-glycoprotein (ABCB1, P-gp) expression compared to A2780 cells.
32355733	8	72	dep	P-glycoprotein	1179:1192	arg1	ABCB1					1195:1199	ABCB1	1195:1199	ABCB1	1195:1199	Cisplatin-resistant variants were confirmed by higher half maximal inhibitory concentration (IC50) values and increased P-glycoprotein (ABCB1, P-gp) expression compared to A2780 cells.
32355733	5	73	theme	ovarian	737:743	arg1	cells					752:756	ovarian cancer cells	737:756	ovarian cancer cells	737:756	Therefore, the objective of this study was to determine platinum resistance-related N-glycans in ovarian cancer cells during continuous exposure to cisplatin.
32355733	9	74	from	resistance	1318:1327	arg1	variants					1352:1359	cisplatin-resistant variants	1332:1359	cisplatin-resistant variants	1332:1359	Analysis of dynamic N-glycomic changes during the development of platinum resistance in cisplatin-resistant variants was performed with MALDI-time-of-flight (TOF)-MS combined with ethyl esterification derivatization, which were used to discriminate between α2,3- and α2,6-linkage N-acetylneuraminic acid.
32355733	13	75	theme	individualized	2291:2304	arg1	treatment					2306:2314	individualized treatment	2291:2314	individualized treatment of ovarian cancer patients	2291:2341	CONCLUSIONS Analysis of N-glycans and glycogene expression showed that α2,3-linked sialic structures might serve as biomarkers to monitor the development of platinum resistance and to guide individualized treatment of ovarian cancer patients.
32355733	4	76	theme	glycomic	541:548	arg1	alterations					550:560	glycomic alterations	541:560	glycomic alterations during platinum resistance development in ovarian cancer	541:617	Moreover, glycomic alterations during platinum resistance development in ovarian cancer are rarely reported.
32355733	0	77	theme	ovarian	80:86	arg1	line					100:103	ovarian cancer cell line A2780	80:109	ovarian cancer cell line A2780	80:109	Dynamic analysis of N-glycomic and transcriptomic changes in the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants.
32355733	13	78	theme	resistance	2267:2276	arg1	development					2243:2253	the development	2239:2253	the development of platinum resistance	2239:2276	CONCLUSIONS Analysis of N-glycans and glycogene expression showed that α2,3-linked sialic structures might serve as biomarkers to monitor the development of platinum resistance and to guide individualized treatment of ovarian cancer patients.
32355733	8	79	theme	inhibitory	1126:1135	arg1	values					1158:1163	half maximal inhibitory concentration (IC50) values	1113:1163	half maximal inhibitory concentration (IC50) values	1113:1163	Cisplatin-resistant variants were confirmed by higher half maximal inhibitory concentration (IC50) values and increased P-glycoprotein (ABCB1, P-gp) expression compared to A2780 cells.
32355733	1	80	theme	BACKGROUND	154:163	arg1	process					210:216	a dynamic process	200:216	a dynamic process that occurs during continuous chemotherapy and contributes to high mortality in ovarian cancer	200:311	BACKGROUND Platinum resistance development is a dynamic process that occurs during continuous chemotherapy and contributes to high mortality in ovarian cancer.
32355733	1	80	theme	BACKGROUND	154:163	arg1	development					185:195	BACKGROUND Platinum resistance development	154:195	BACKGROUND Platinum resistance development	154:195	BACKGROUND Platinum resistance development is a dynamic process that occurs during continuous chemotherapy and contributes to high mortality in ovarian cancer.
32355733	0	81	from	analysis	8:15	arg1	development					65:75	the development	61:75	the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants	61:151	Dynamic analysis of N-glycomic and transcriptomic changes in the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants.
32355733	0	82	theme	cell	95:98	arg1	line					100:103	ovarian cancer cell line A2780	80:109	ovarian cancer cell line A2780	80:109	Dynamic analysis of N-glycomic and transcriptomic changes in the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants.
32355733	9	83	theme	ethyl	1424:1428	arg1	derivatization					1445:1458	ethyl esterification derivatization	1424:1458	ethyl esterification derivatization	1424:1458	Analysis of dynamic N-glycomic changes during the development of platinum resistance in cisplatin-resistant variants was performed with MALDI-time-of-flight (TOF)-MS combined with ethyl esterification derivatization, which were used to discriminate between α2,3- and α2,6-linkage N-acetylneuraminic acid.
32355733	3	84	theme	Many	379:382	arg1	studies					384:390	Many studies	379:390	Many studies on platinum resistance	379:413	Many studies on platinum resistance have been performed, but few of them have investigated platinum resistance-associated glycans based on N-glycomics.
32355733	1	85	theme	continuous	237:246	arg1	chemotherapy					248:259	continuous chemotherapy	237:259	continuous chemotherapy	237:259	BACKGROUND Platinum resistance development is a dynamic process that occurs during continuous chemotherapy and contributes to high mortality in ovarian cancer.
32355733	10	86	theme	glycotransferase	1598:1613	arg1	level					1615:1619	a glycotransferase level	1596:1619	a glycotransferase level	1596:1619	N-glycan alterations were further validated on a glycotransferase level via transcriptome sequencing and real-time PCR (RT-PCR).
32355733	3	87	theme	them	447:450	arg1	few					440:442	few	440:442	few	440:442	Many studies on platinum resistance have been performed, but few of them have investigated platinum resistance-associated glycans based on N-glycomics.
32355733	0	88	theme	N-glycomic	20:29	arg1	changes					50:56	N-glycomic and transcriptomic changes	20:56	N-glycomic and transcriptomic changes in the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants	20:151	Dynamic analysis of N-glycomic and transcriptomic changes in the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants.
32355733	1	89	theme	ovarian	298:304	arg1	cancer					306:311	ovarian cancer	298:311	ovarian cancer	298:311	BACKGROUND Platinum resistance development is a dynamic process that occurs during continuous chemotherapy and contributes to high mortality in ovarian cancer.
32355733	8	90	theme	P-glycoprotein	1179:1192	arg1	expression					1208:1217	increased P-glycoprotein (ABCB1, P-gp) expression	1169:1217	increased P-glycoprotein (ABCB1, P-gp) expression	1169:1217	Cisplatin-resistant variants were confirmed by higher half maximal inhibitory concentration (IC50) values and increased P-glycoprotein (ABCB1, P-gp) expression compared to A2780 cells.
32355733	13	91	theme	sialic	2184:2189	arg1	structures					2191:2200	α2,3-linked sialic structures	2172:2200	α2,3-linked sialic structures	2172:2200	CONCLUSIONS Analysis of N-glycans and glycogene expression showed that α2,3-linked sialic structures might serve as biomarkers to monitor the development of platinum resistance and to guide individualized treatment of ovarian cancer patients.
32355733	13	91	theme	sialic	2184:2189	arg1	biomarkers					2217:2226	biomarkers	2217:2226	biomarkers	2217:2226	CONCLUSIONS Analysis of N-glycans and glycogene expression showed that α2,3-linked sialic structures might serve as biomarkers to monitor the development of platinum resistance and to guide individualized treatment of ovarian cancer patients.
32355733	10	92	theme	real-time	1654:1662	arg1	RT-PCR					1669:1674	RT-PCR	1669:1674	RT-PCR	1669:1674	N-glycan alterations were further validated on a glycotransferase level via transcriptome sequencing and real-time PCR (RT-PCR).
32355733	10	92	theme	real-time	1654:1662	arg1	PCR					1664:1666	real-time PCR	1654:1666	real-time PCR (RT-PCR)	1654:1675	N-glycan alterations were further validated on a glycotransferase level via transcriptome sequencing and real-time PCR (RT-PCR).
32355733	0	93	theme	transcriptomic	35:48	arg1	changes					50:56	N-glycomic and transcriptomic changes	20:56	N-glycomic and transcriptomic changes in the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants	20:151	Dynamic analysis of N-glycomic and transcriptomic changes in the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants.
32355733	8	94	dep	higher	1106:1111	arg1	values					1158:1163	half maximal inhibitory concentration (IC50) values	1113:1163	half maximal inhibitory concentration (IC50) values	1113:1163	Cisplatin-resistant variants were confirmed by higher half maximal inhibitory concentration (IC50) values and increased P-glycoprotein (ABCB1, P-gp) expression compared to A2780 cells.
32355733	4	95	theme	resistance	578:587	arg1	development					589:599	platinum resistance development	569:599	platinum resistance development in ovarian cancer	569:617	Moreover, glycomic alterations during platinum resistance development in ovarian cancer are rarely reported.
32355733	3	96	theme	resistance-associated	479:499	arg1	glycans					501:507	platinum resistance-associated glycans	470:507	platinum resistance-associated glycans based on N-glycomics	470:528	Many studies on platinum resistance have been performed, but few of them have investigated platinum resistance-associated glycans based on N-glycomics.
32355733	12	97	theme	FUT11	2021:2025	arg1	expression					2007:2016	gene expression	2002:2016	gene expression of FUT11, FUT1, GMDS, and B4GALT5	2002:2050	Transcriptome sequencing and RT-PCR showed that gene expression of ST3GAL6 and MGAT4A increased, while gene expression of FUT11, FUT1, GMDS, and B4GALT5 decreased in three cisplatin-resistant variants.
32355733	12	98	theme	B4GALT5	2044:2050	arg1	expression					2007:2016	gene expression	2002:2016	gene expression of FUT11, FUT1, GMDS, and B4GALT5	2002:2050	Transcriptome sequencing and RT-PCR showed that gene expression of ST3GAL6 and MGAT4A increased, while gene expression of FUT11, FUT1, GMDS, and B4GALT5 decreased in three cisplatin-resistant variants.
32355733	2	99	theme	platinum	358:365	arg1	resistance					367:376	platinum resistance	358:376	platinum resistance	358:376	Abnormal glycosylation has been reported in platinum resistance.
32355733	11	100	link	α2,3-linked	1742:1752	arg1	structures					1761:1770	α2,3-linked sialic structures	1742:1770	α2,3-linked sialic structures	1742:1770	RESULTS Compared to the A2780 cells, MS analysis indicated that α2,3-linked sialic structures and N-glycan gal-ratios were significantly higher, while fucosylated glycans were lower in three cisplatin-resistant variants.
32355733	12	101	theme	FUT1	2028:2031	arg1	expression					2007:2016	gene expression	2002:2016	gene expression of FUT11, FUT1, GMDS, and B4GALT5	2002:2050	Transcriptome sequencing and RT-PCR showed that gene expression of ST3GAL6 and MGAT4A increased, while gene expression of FUT11, FUT1, GMDS, and B4GALT5 decreased in three cisplatin-resistant variants.
32355733	12	102	theme	gene	2002:2005	arg1	expression					2007:2016	gene expression	2002:2016	gene expression of FUT11, FUT1, GMDS, and B4GALT5	2002:2050	Transcriptome sequencing and RT-PCR showed that gene expression of ST3GAL6 and MGAT4A increased, while gene expression of FUT11, FUT1, GMDS, and B4GALT5 decreased in three cisplatin-resistant variants.
32355733	11	103	theme	cisplatin-resistant	1869:1887	arg1	variants					1889:1896	three cisplatin-resistant variants	1863:1896	three cisplatin-resistant variants	1863:1896	RESULTS Compared to the A2780 cells, MS analysis indicated that α2,3-linked sialic structures and N-glycan gal-ratios were significantly higher, while fucosylated glycans were lower in three cisplatin-resistant variants.
32355733	13	104	theme	α2,3-linked	2172:2182	arg1	structures					2191:2200	α2,3-linked sialic structures	2172:2200	α2,3-linked sialic structures	2172:2200	CONCLUSIONS Analysis of N-glycans and glycogene expression showed that α2,3-linked sialic structures might serve as biomarkers to monitor the development of platinum resistance and to guide individualized treatment of ovarian cancer patients.
32355733	13	104	theme	α2,3-linked	2172:2182	arg1	biomarkers					2217:2226	biomarkers	2217:2226	biomarkers	2217:2226	CONCLUSIONS Analysis of N-glycans and glycogene expression showed that α2,3-linked sialic structures might serve as biomarkers to monitor the development of platinum resistance and to guide individualized treatment of ovarian cancer patients.
32355733	7	105	theme	platinum	970:977	arg1	resistance					979:988	platinum resistance	970:988	platinum resistance	970:988	METHODS This study mimicked the development of platinum resistance in ovarian cancer by continuously exposing A2780 cells to cisplatin.
32355733	9	106	theme	α2,6-linkage	1511:1522	arg1	acid					1543:1546	α2,6-linkage N-acetylneuraminic acid	1511:1546	α2,6-linkage N-acetylneuraminic acid	1511:1546	Analysis of dynamic N-glycomic changes during the development of platinum resistance in cisplatin-resistant variants was performed with MALDI-time-of-flight (TOF)-MS combined with ethyl esterification derivatization, which were used to discriminate between α2,3- and α2,6-linkage N-acetylneuraminic acid.
32355733	12	107	theme	ST3GAL6	1966:1972	arg1	expression					1952:1961	gene expression	1947:1961	gene expression of ST3GAL6 and MGAT4A	1947:1983	Transcriptome sequencing and RT-PCR showed that gene expression of ST3GAL6 and MGAT4A increased, while gene expression of FUT11, FUT1, GMDS, and B4GALT5 decreased in three cisplatin-resistant variants.
32355733	11	108	theme	sialic	1754:1759	arg1	structures					1761:1770	α2,3-linked sialic structures	1742:1770	α2,3-linked sialic structures	1742:1770	RESULTS Compared to the A2780 cells, MS analysis indicated that α2,3-linked sialic structures and N-glycan gal-ratios were significantly higher, while fucosylated glycans were lower in three cisplatin-resistant variants.
33080875	8	0	with	hydrogels	1468:1476	arg1	betamethasone					1496:1508	encapsulated betamethasone	1483:1508	encapsulated betamethasone	1483:1508	Furthermore, Ty-GG/SF hydrogels with encapsulated betamethasone revealed greater therapeutic efficacy than the drug applied alone.
33080875	4	1	theme	synthetic	503:511	arg1	materials					513:521	synthetic materials	503:521	synthetic materials such as gellan gum	503:540	Thus, synthetic materials such as gellan gum can be used to produce composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties.
33080875	4	1	theme	synthetic	503:511	arg1	gum					538:540	gellan gum	531:540	gellan gum	531:540	Thus, synthetic materials such as gellan gum can be used to produce composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties.
33080875	8	2	theme	encapsulated	1483:1494	arg1	betamethasone					1496:1508	encapsulated betamethasone	1483:1508	encapsulated betamethasone	1483:1508	Furthermore, Ty-GG/SF hydrogels with encapsulated betamethasone revealed greater therapeutic efficacy than the drug applied alone.
33080875	5	3	theme	mechanical	909:918	arg1	properties					920:929	mechanical properties	909:929	mechanical properties	909:929	In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Ty-GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA).
33080875	2	4	from	versatility	315:325	arg1	medicine					363:370	medicine	363:370	medicine	363:370	Silk fibroin has been extensively studied for use in drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine.
33080875	6	5	theme	Ty-GG/SF	1014:1021	arg1	hydrogels					1023:1031	The Ty-GG/SF hydrogels	1010:1031	The Ty-GG/SF hydrogels	1010:1031	The Ty-GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2-5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time.
33080875	2	6	from	record	353:358	arg1	medicine					363:370	medicine	363:370	medicine	363:370	Silk fibroin has been extensively studied for use in drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine.
33080875	7	7	dep	In	1300:1301	arg1	vitro					1303:1307	vitro	1303:1307	vitro	1303:1307	In vitro studies showed that Ty-GG/SF hydrogels did not produce a deleterious effect on cellular metabolic activity, morphology or proliferation.
33080875	7	8	theme	metabolic	1397:1405	arg1	activity					1407:1414	cellular metabolic activity	1388:1414	cellular metabolic activity	1388:1414	In vitro studies showed that Ty-GG/SF hydrogels did not produce a deleterious effect on cellular metabolic activity, morphology or proliferation.
33080875	4	9	used	used	549:552	arg2	materials					513:521	synthetic materials	503:521	synthetic materials such as gellan gum	503:540	Thus, synthetic materials such as gellan gum can be used to produce composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties.
33080875	4	9	used	used	549:552	arg2	gum					538:540	gellan gum	531:540	gellan gum	531:540	Thus, synthetic materials such as gellan gum can be used to produce composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties.
33080875	3	10	theme	polymeric	402:410	arg1	materials					412:420	natural polymeric materials	394:420	natural polymeric materials	394:420	However, in general, natural polymeric materials are not mechanically stable enough, and have high rates of biodegradation.
33080875	5	11	theme	rheumatoid	983:992	arg1	arthritis					994:1002	rheumatoid arthritis	983:1002	rheumatoid arthritis (RA)	983:1007	In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Ty-GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA).
33080875	5	11	theme	rheumatoid	983:992	arg1	RA					1005:1006	RA	1005:1006	RA	1005:1006	In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Ty-GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA).
33080875	5	12	theme	therapeutic	953:963	arg1	efficacy					965:972	therapeutic efficacy	953:972	therapeutic efficacy to treat rheumatoid arthritis (RA)	953:1007	In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Ty-GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA).
33080875	3	13	from	general	385:391	arg1	stable					443:448	stable	443:448	stable	443:448	However, in general, natural polymeric materials are not mechanically stable enough, and have high rates of biodegradation.
33080875	9	14	theme	arthritis	1731:1739	arg1	treatment					1707:1715	the treatment	1703:1715	the treatment of rheumatoid arthritis	1703:1739	Therefore, this strategy can provide an improvement in therapeutic efficacy when compared to the traditional use of drugs for the treatment of rheumatoid arthritis.
33080875	7	15	from	effect	1378:1383	arg1	proliferation					1431:1443	proliferation	1431:1443	proliferation	1431:1443	In vitro studies showed that Ty-GG/SF hydrogels did not produce a deleterious effect on cellular metabolic activity, morphology or proliferation.
33080875	7	15	from	effect	1378:1383	arg1	morphology					1417:1426	morphology	1417:1426	morphology	1417:1426	In vitro studies showed that Ty-GG/SF hydrogels did not produce a deleterious effect on cellular metabolic activity, morphology or proliferation.
33080875	7	15	from	effect	1378:1383	arg1	activity					1407:1414	cellular metabolic activity	1388:1414	cellular metabolic activity	1388:1414	In vitro studies showed that Ty-GG/SF hydrogels did not produce a deleterious effect on cellular metabolic activity, morphology or proliferation.
33080875	4	16	theme	biological	591:600	arg1	signals					602:608	biological signals	591:608	biological signals	591:608	Thus, synthetic materials such as gellan gum can be used to produce composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties.
33080875	5	17	theme	gellan	766:771	arg1	gum					773:775	tyramine-modified gellan gum	748:775	tyramine-modified gellan gum	748:775	In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Ty-GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA).
33080875	6	18	with	profile	1281:1287	arg1	component					1232:1240	a significant solid component	1212:1240	a significant solid component	1212:1240	The Ty-GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2-5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time.
33080875	6	19	theme	viscoelastic	1185:1196	arg1	capacity					1198:1205	viscoelastic capacity	1185:1205	viscoelastic capacity with a significant solid component	1185:1240	The Ty-GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2-5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time.
33080875	5	20	theme	gum	773:775	arg1	hydrogels					735:743	hydrogels	735:743	hydrogels of tyramine-modified gellan gum with silk fibroin (Ty-GG/SF)	735:804	In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Ty-GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA).
33080875	6	21	with	resistance	1114:1123	arg1	component					1232:1240	a significant solid component	1212:1240	a significant solid component	1212:1240	The Ty-GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2-5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time.
33080875	1	22	theme	Rheumatoid	117:126	arg1	disease					153:159	a rheumatic disease	141:159	a rheumatic disease for which a healing treatment does not presently exist	141:214	Rheumatoid arthritis is a rheumatic disease for which a healing treatment does not presently exist.
33080875	1	22	theme	Rheumatoid	117:126	arg1	arthritis					128:136	Rheumatoid arthritis	117:136	Rheumatoid arthritis	117:136	Rheumatoid arthritis is a rheumatic disease for which a healing treatment does not presently exist.
33080875	4	23	theme	mechanical	678:687	arg1	properties					689:698	the desired mechanical properties	666:698	the desired mechanical properties	666:698	Thus, synthetic materials such as gellan gum can be used to produce composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties.
33080875	3	24	theme	natural	394:400	arg1	materials					412:420	natural polymeric materials	394:420	natural polymeric materials	394:420	However, in general, natural polymeric materials are not mechanically stable enough, and have high rates of biodegradation.
33080875	8	25	theme	Ty-GG/SF	1459:1466	arg1	hydrogels					1468:1476	Ty-GG/SF hydrogels	1459:1476	Ty-GG/SF hydrogels with encapsulated betamethasone	1459:1508	Furthermore, Ty-GG/SF hydrogels with encapsulated betamethasone revealed greater therapeutic efficacy than the drug applied alone.
33080875	2	26	theme	clinical	338:345	arg1	record					353:358	strong clinical track record	331:358	strong clinical track record	331:358	Silk fibroin has been extensively studied for use in drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine.
33080875	2	27	from	use	263:265	arg1	systems					284:290	drug delivery systems	270:290	drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine	270:370	Silk fibroin has been extensively studied for use in drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine.
33080875	0	28	theme	Anti-Inflammatory	0:16	arg1	Properties					18:27	Anti-Inflammatory Properties	0:27	Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified	0:80	Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels.
33080875	6	29	theme	significant	1214:1224	arg1	component					1232:1240	a significant solid component	1212:1240	a significant solid component	1212:1240	The Ty-GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2-5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time.
33080875	9	30	theme	drugs	1693:1697	arg1	use					1686:1688	the traditional use	1670:1688	the traditional use of drugs for the treatment of rheumatoid arthritis	1670:1739	Therefore, this strategy can provide an improvement in therapeutic efficacy when compared to the traditional use of drugs for the treatment of rheumatoid arthritis.
33080875	2	31	theme	strong	331:336	arg1	record					353:358	strong clinical track record	331:358	strong clinical track record	331:358	Silk fibroin has been extensively studied for use in drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine.
33080875	6	32	theme	gelation	1079:1086	arg1	time					1088:1091	gelation time	1079:1091	gelation time around 2-5 min	1079:1106	The Ty-GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2-5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time.
33080875	0	33	theme	Injectable	32:41	arg1	Tyramine-Modified					64:80	Injectable Betamethasone-Loaded Tyramine-Modified	32:80	Injectable Betamethasone-Loaded Tyramine-Modified	32:80	Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels.
33080875	5	34	theme	encapsulated	845:856	arg1	betamethasone					858:870	encapsulated betamethasone	845:870	encapsulated betamethasone	845:870	In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Ty-GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA).
33080875	0	35	dep	Hydrogels	106:114	arg1	Properties					18:27	Anti-Inflammatory Properties	0:27	Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified	0:80	Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels.
33080875	6	36	theme	injectability	1162:1174	arg1	profile					1176:1182	a suitable injectability profile	1151:1182	a suitable injectability profile	1151:1182	The Ty-GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2-5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time.
33080875	4	37	theme	composite	565:573	arg1	structures					575:584	composite structures	565:584	composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties	565:698	Thus, synthetic materials such as gellan gum can be used to produce composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties.
33080875	2	38	theme	Silk	217:220	arg1	fibroin					222:228	Silk fibroin	217:228	Silk fibroin	217:228	Silk fibroin has been extensively studied for use in drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine.
33080875	1	39	theme	rheumatic	143:151	arg1	disease					153:159	a rheumatic disease	141:159	a rheumatic disease for which a healing treatment does not presently exist	141:214	Rheumatoid arthritis is a rheumatic disease for which a healing treatment does not presently exist.
33080875	1	39	theme	rheumatic	143:151	arg1	arthritis					128:136	Rheumatoid arthritis	117:136	Rheumatoid arthritis	117:136	Rheumatoid arthritis is a rheumatic disease for which a healing treatment does not presently exist.
33080875	5	40	with	hydrogels	735:743	arg1	Ty-GG/SF					796:803	Ty-GG/SF	796:803	Ty-GG/SF	796:803	In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Ty-GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA).
33080875	5	40	with	hydrogels	735:743	arg1	fibroin					787:793	silk fibroin	782:793	silk fibroin (Ty-GG/SF)	782:804	In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Ty-GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA).
33080875	0	41	theme	Tyramine-Modified	64:80	arg1	Properties					18:27	Anti-Inflammatory Properties	0:27	Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified	0:80	Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels.
33080875	6	42	with	capacity	1198:1205	arg1	component					1232:1240	a significant solid component	1212:1240	a significant solid component	1212:1240	The Ty-GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2-5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time.
33080875	3	43	contain	have	462:465	arg2	rates					472:476	high rates	467:476	high rates of biodegradation	467:494	However, in general, natural polymeric materials are not mechanically stable enough, and have high rates of biodegradation.
33080875	3	43	contain	have	462:465	arg1	materials					412:420	natural polymeric materials	394:420	natural polymeric materials	394:420	However, in general, natural polymeric materials are not mechanically stable enough, and have high rates of biodegradation.
33080875	6	44	theme	release	1273:1279	arg1	profile					1281:1287	a betamethasone-controlled release profile	1246:1287	a betamethasone-controlled release profile over time	1246:1297	The Ty-GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2-5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time.
33080875	7	45	theme	Ty-GG/SF	1329:1336	arg1	hydrogels					1338:1346	Ty-GG/SF hydrogels	1329:1346	Ty-GG/SF hydrogels	1329:1346	In vitro studies showed that Ty-GG/SF hydrogels did not produce a deleterious effect on cellular metabolic activity, morphology or proliferation.
33080875	4	46	theme	desired	670:676	arg1	properties					689:698	the desired mechanical properties	666:698	the desired mechanical properties	666:698	Thus, synthetic materials such as gellan gum can be used to produce composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties.
33080875	0	47	theme	Betamethasone-Loaded	43:62	arg1	Tyramine-Modified					64:80	Injectable Betamethasone-Loaded Tyramine-Modified	32:80	Injectable Betamethasone-Loaded Tyramine-Modified	32:80	Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels.
33080875	5	48	theme	tyramine-modified	748:764	arg1	gum					773:775	tyramine-modified gellan gum	748:775	tyramine-modified gellan gum	748:775	In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Ty-GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA).
33080875	6	49	theme	betamethasone-controlled	1248:1271	arg1	profile					1281:1287	a betamethasone-controlled release profile	1246:1287	a betamethasone-controlled release profile over time	1246:1297	The Ty-GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2-5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time.
33080875	3	50	theme	high	467:470	arg1	rates					472:476	high rates	467:476	high rates of biodegradation	467:494	However, in general, natural polymeric materials are not mechanically stable enough, and have high rates of biodegradation.
33080875	4	51	theme	gellan	531:536	arg1	gum					538:540	gellan gum	531:540	gellan gum	531:540	Thus, synthetic materials such as gellan gum can be used to produce composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties.
33080875	8	52	theme	greater	1519:1525	arg1	efficacy					1539:1546	greater therapeutic efficacy	1519:1546	greater therapeutic efficacy than the drug applied alone	1519:1574	Furthermore, Ty-GG/SF hydrogels with encapsulated betamethasone revealed greater therapeutic efficacy than the drug applied alone.
33080875	9	53	theme	traditional	1674:1684	arg1	use					1686:1688	the traditional use	1670:1688	the traditional use of drugs for the treatment of rheumatoid arthritis	1670:1739	Therefore, this strategy can provide an improvement in therapeutic efficacy when compared to the traditional use of drugs for the treatment of rheumatoid arthritis.
33080875	5	54	theme	horseradish	810:820	arg1	HRP					834:836	HRP	834:836	HRP	834:836	In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Ty-GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA).
33080875	5	54	theme	horseradish	810:820	arg1	peroxidase					822:831	horseradish peroxidase	810:831	horseradish peroxidase (HRP)	810:837	In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Ty-GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA).
33080875	6	55	with	profile	1176:1182	arg1	component					1232:1240	a significant solid component	1212:1240	a significant solid component	1212:1240	The Ty-GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2-5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time.
33080875	7	56	theme	deleterious	1366:1376	arg1	effect					1378:1383	a deleterious effect	1364:1383	a deleterious effect on cellular metabolic activity, morphology or proliferation	1364:1443	In vitro studies showed that Ty-GG/SF hydrogels did not produce a deleterious effect on cellular metabolic activity, morphology or proliferation.
33080875	8	57	theme	therapeutic	1527:1537	arg1	efficacy					1539:1546	greater therapeutic efficacy	1519:1546	greater therapeutic efficacy than the drug applied alone	1519:1574	Furthermore, Ty-GG/SF hydrogels with encapsulated betamethasone revealed greater therapeutic efficacy than the drug applied alone.
33080875	4	58	with	structures	575:584	arg1	signals					602:608	biological signals	591:608	biological signals	591:608	Thus, synthetic materials such as gellan gum can be used to produce composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties.
33080875	7	59	theme	cellular	1388:1395	arg1	activity					1407:1414	cellular metabolic activity	1388:1414	cellular metabolic activity	1388:1414	In vitro studies showed that Ty-GG/SF hydrogels did not produce a deleterious effect on cellular metabolic activity, morphology or proliferation.
33080875	2	60	theme	due	292:294	arg1	systems					284:290	drug delivery systems	270:290	drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine	270:370	Silk fibroin has been extensively studied for use in drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine.
33080875	3	61	theme	biodegradation	481:494	arg1	rates					472:476	high rates	467:476	high rates of biodegradation	467:494	However, in general, natural polymeric materials are not mechanically stable enough, and have high rates of biodegradation.
33080875	6	62	theme	suitable	1153:1160	arg1	profile					1176:1182	a suitable injectability profile	1151:1182	a suitable injectability profile	1151:1182	The Ty-GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2-5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time.
33080875	6	63	theme	β-sheet	1045:1051	arg1	structure					1063:1071	a β-sheet secondary structure	1043:1071	a β-sheet secondary structure	1043:1071	The Ty-GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2-5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time.
33080875	6	64	theme	solid	1226:1230	arg1	component					1232:1240	a significant solid component	1212:1240	a significant solid component	1212:1240	The Ty-GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2-5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time.
33080875	3	65	from	stable	443:448	arg1	general					385:391	general	385:391	general	385:391	However, in general, natural polymeric materials are not mechanically stable enough, and have high rates of biodegradation.
33080875	2	66	theme	track	347:351	arg1	record					353:358	strong clinical track record	331:358	strong clinical track record	331:358	Silk fibroin has been extensively studied for use in drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine.
33080875	6	67	theme	enzymatic	1128:1136	arg1	degradation					1138:1148	enzymatic degradation	1128:1148	enzymatic degradation	1128:1148	The Ty-GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2-5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time.
33080875	9	68	theme	rheumatoid	1720:1729	arg1	arthritis					1731:1739	rheumatoid arthritis	1720:1739	rheumatoid arthritis	1720:1739	Therefore, this strategy can provide an improvement in therapeutic efficacy when compared to the traditional use of drugs for the treatment of rheumatoid arthritis.
33080875	6	69	theme	secondary	1053:1061	arg1	structure					1063:1071	a β-sheet secondary structure	1043:1071	a β-sheet secondary structure	1043:1071	The Ty-GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2-5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time.
33080875	2	70	theme	delivery	275:282	arg1	systems					284:290	drug delivery systems	270:290	drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine	270:370	Silk fibroin has been extensively studied for use in drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine.
33080875	1	71	theme	healing	173:179	arg1	treatment					181:189	a healing treatment	171:189	a healing treatment	171:189	Rheumatoid arthritis is a rheumatic disease for which a healing treatment does not presently exist.
33080875	2	72	from	uniqueness	303:312	arg1	medicine					363:370	medicine	363:370	medicine	363:370	Silk fibroin has been extensively studied for use in drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine.
33080875	9	73	theme	therapeutic	1632:1642	arg1	efficacy					1644:1651	therapeutic efficacy	1632:1651	therapeutic efficacy	1632:1651	Therefore, this strategy can provide an improvement in therapeutic efficacy when compared to the traditional use of drugs for the treatment of rheumatoid arthritis.
33080875	5	74	theme	silk	782:785	arg1	Ty-GG/SF					796:803	Ty-GG/SF	796:803	Ty-GG/SF	796:803	In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Ty-GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA).
33080875	5	74	theme	silk	782:785	arg1	fibroin					787:793	silk fibroin	782:793	silk fibroin (Ty-GG/SF)	782:804	In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Ty-GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA).
33080875	2	75	theme	drug	270:273	arg1	systems					284:290	drug delivery systems	270:290	drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine	270:370	Silk fibroin has been extensively studied for use in drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine.
33080875	7	76	theme	In	1300:1301	arg1	studies					1309:1315	In vitro studies	1300:1315	In vitro studies	1300:1315	In vitro studies showed that Ty-GG/SF hydrogels did not produce a deleterious effect on cellular metabolic activity, morphology or proliferation.
33080875	6	77	theme	good	1109:1112	arg1	resistance					1114:1123	good resistance	1109:1123	good resistance to enzymatic degradation	1109:1148	The Ty-GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2-5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time.
33080875	9	78	from	improvement	1617:1627	arg1	efficacy					1644:1651	therapeutic efficacy	1632:1651	therapeutic efficacy	1632:1651	Therefore, this strategy can provide an improvement in therapeutic efficacy when compared to the traditional use of drugs for the treatment of rheumatoid arthritis.
33080875	4	79	theme	tissue-specific	621:635	arg1	interactions					637:648	tissue-specific interactions	621:648	tissue-specific interactions	621:648	Thus, synthetic materials such as gellan gum can be used to produce composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties.
34428384	3	0	theme	thiolated	682:690	arg1	based					647:651	a novel hydrogel composite based	620:651	a novel hydrogel composite based on the polymer	620:666	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	3	0	theme	thiolated	682:690	arg1	chitosan					692:699	cross-linked thiolated chitosan	669:699	cross-linked thiolated chitosan	669:699	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	6	1	theme	TCS/CMC	1303:1309	arg1	hydrogels					1311:1319	TCS/CMC hydrogels	1303:1319	TCS/CMC hydrogels	1303:1319	TCS/CMC hydrogels maintain more than 90% of their weight and retain their original form after 21 days.
34428384	1	2	theme	processes	276:284	arg1	range					258:262	a wide range	251:262	a wide range of cellular processes as well as tissue regeneration	251:315	Growth factors (GFs) are soluble proteins extracellular that control a wide range of cellular processes as well as tissue regeneration.
34428384	1	2	theme	processes	276:284	arg1	regeneration					304:315	tissue regeneration	297:315	a wide range of cellular processes as well as tissue regeneration	251:315	Growth factors (GFs) are soluble proteins extracellular that control a wide range of cellular processes as well as tissue regeneration.
34428384	4	3	theme	elastic	896:902	arg1	modulus					904:910	the elastic modulus	892:910	the elastic modulus of TCS/CMC hydrogels with an optimized CMC concentration	892:967	Rheological measurements showed that the elastic modulus of TCS/CMC hydrogels with an optimized CMC concentration could reach around 2.5 kPa or higher than their respective viscous modulus, indicating that they behaved like strong hydrogels.
34428384	4	4	theme	strong	1079:1084	arg1	hydrogels					1086:1094	strong hydrogels	1079:1094	strong hydrogels	1079:1094	Rheological measurements showed that the elastic modulus of TCS/CMC hydrogels with an optimized CMC concentration could reach around 2.5 kPa or higher than their respective viscous modulus, indicating that they behaved like strong hydrogels.
34428384	8	5	theme	TGF-β1-loaded	1623:1635	arg1	hydrogels					1645:1653	TGF-β1-loaded TCS/CMC hydrogels	1623:1653	TGF-β1-loaded TCS/CMC hydrogels	1623:1653	On day 21, BMSC treated with TGF-β1 in medium or TGF-β1-loaded TCS/CMC hydrogels grew faster than the other groups.
34428384	5	6	theme	water	1237:1241	arg1	content					1243:1249	water content	1237:1249	water content	1237:1249	Crosslinking significantly alters the overall network distribution, surface morphology, pore size, porosity, gelation time, swelling ratio, water content, and in vitro degradation of the TCS/CMC hydrogels.
34428384	7	7	theme	TCS/CMC	1438:1444	arg1	hydrogels					1446:1454	TCS/CMC hydrogels	1438:1454	TCS/CMC hydrogels	1438:1454	TGF-β1 released marginally from TCS/CMC hydrogels as incubation time increased, up to 21 days, with around 18.6 ± 0.9% of the drug stored inside the TCS/CMC hydrogels.
34428384	10	8	theme	novel	1930:1934	arg1	strategy					1948:1955	a novel therapeutic strategy	1928:1955	a novel therapeutic strategy for repairing articular cartilage defects	1928:1997	The optimal ability of this novel TGF-β1-loaded TCS/CMC hydrogel system was further demonstrated by histological analysis, resulting in a novel therapeutic strategy for repairing articular cartilage defects.
34428384	5	9	theme	swelling	1221:1228	arg1	ratio					1230:1234	swelling ratio	1221:1234	swelling ratio	1221:1234	Crosslinking significantly alters the overall network distribution, surface morphology, pore size, porosity, gelation time, swelling ratio, water content, and in vitro degradation of the TCS/CMC hydrogels.
34428384	3	10	theme	TCS/CMC	740:746	arg1	system					758:763	CMC (TCS/CMC) hydrogel system	735:763	CMC (TCS/CMC) hydrogel system	735:763	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	3	10	theme	TCS/CMC	740:746	arg1	carriers					799:806	injectable TGF-β1 carriers	781:806	injectable TGF-β1 carriers for cartilage tissue engineering applications	781:852	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	2	11	theme	growth	337:342	arg1	TGF-β1					359:364	TGF-β1	359:364	TGF-β1	359:364	While transforming growth factor beta-1 (TGF-β1) promotes chondrogenesis, its medical use is restricted by its potential protein instability, which necessitates high doses of the protein, which can result in adverse side effects such as inefficient cartilage formation.
34428384	2	11	theme	growth	337:342	arg1	beta-1					351:356	transforming growth factor beta-1	324:356	transforming growth factor beta-1 (TGF-β1)	324:365	While transforming growth factor beta-1 (TGF-β1) promotes chondrogenesis, its medical use is restricted by its potential protein instability, which necessitates high doses of the protein, which can result in adverse side effects such as inefficient cartilage formation.
34428384	1	12	theme	soluble	207:213	arg1	factors					189:195	Growth factors	182:195	Growth factors (GFs)	182:201	Growth factors (GFs) are soluble proteins extracellular that control a wide range of cellular processes as well as tissue regeneration.
34428384	1	12	theme	soluble	207:213	arg1	proteins					215:222	soluble proteins	207:222	soluble proteins extracellular that control a wide range of cellular processes as well as tissue regeneration	207:315	Growth factors (GFs) are soluble proteins extracellular that control a wide range of cellular processes as well as tissue regeneration.
34428384	2	13	theme	inefficient	555:565	arg1	formation					577:585	inefficient cartilage formation	555:585	inefficient cartilage formation	555:585	While transforming growth factor beta-1 (TGF-β1) promotes chondrogenesis, its medical use is restricted by its potential protein instability, which necessitates high doses of the protein, which can result in adverse side effects such as inefficient cartilage formation.
34428384	3	14	theme	CMC	735:737	arg1	system					758:763	CMC (TCS/CMC) hydrogel system	735:763	CMC (TCS/CMC) hydrogel system	735:763	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	3	14	theme	CMC	735:737	arg1	carriers					799:806	injectable TGF-β1 carriers	781:806	injectable TGF-β1 carriers for cartilage tissue engineering applications	781:852	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	1	15	theme	extracellular	224:236	arg1	factors					189:195	Growth factors	182:195	Growth factors (GFs)	182:201	Growth factors (GFs) are soluble proteins extracellular that control a wide range of cellular processes as well as tissue regeneration.
34428384	1	15	theme	extracellular	224:236	arg1	proteins					215:222	soluble proteins	207:222	soluble proteins extracellular that control a wide range of cellular processes as well as tissue regeneration	207:315	Growth factors (GFs) are soluble proteins extracellular that control a wide range of cellular processes as well as tissue regeneration.
34428384	9	16	theme	in vivo	1694:1700	arg1	repair					1712:1717	in vivo cartilage repair	1694:1717	in vivo cartilage repair	1694:1717	For in vivo cartilage repair, full-thickness cartilage defects were induced on rat knees for 8 weeks.
34428384	0	17	theme	tissue	162:167	arg1	engineering					169:179	cartilage tissue engineering	152:179	cartilage tissue engineering	152:179	Enhanced efficacy of transforming growth factor-β1 loaded an injectable cross-linked thiolated chitosan and carboxymethyl cellulose-based hydrogels for cartilage tissue engineering.
34428384	10	18	theme	optimal	1796:1802	arg1	ability					1804:1810	The optimal ability	1792:1810	The optimal ability of this novel TGF-β1-loaded TCS/CMC hydrogel system	1792:1862	The optimal ability of this novel TGF-β1-loaded TCS/CMC hydrogel system was further demonstrated by histological analysis, resulting in a novel therapeutic strategy for repairing articular cartilage defects.
34428384	1	19	theme	tissue	297:302	arg1	regeneration					304:315	tissue regeneration	297:315	a wide range of cellular processes as well as tissue regeneration	251:315	Growth factors (GFs) are soluble proteins extracellular that control a wide range of cellular processes as well as tissue regeneration.
34428384	9	20	theme	full-thickness	1720:1733	arg1	defects					1745:1751	full-thickness cartilage defects	1720:1751	full-thickness cartilage defects	1720:1751	For in vivo cartilage repair, full-thickness cartilage defects were induced on rat knees for 8 weeks.
34428384	3	21	theme	hydrogel	749:756	arg1	system					758:763	CMC (TCS/CMC) hydrogel system	735:763	CMC (TCS/CMC) hydrogel system	735:763	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	3	21	theme	hydrogel	749:756	arg1	carriers					799:806	injectable TGF-β1 carriers	781:806	injectable TGF-β1 carriers for cartilage tissue engineering applications	781:852	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	7	22	dep	21 days	1492:1498	arg1	up					1486:1487	up	1486:1487	up	1486:1487	TGF-β1 released marginally from TCS/CMC hydrogels as incubation time increased, up to 21 days, with around 18.6 ± 0.9% of the drug stored inside the TCS/CMC hydrogels.
34428384	4	23	theme	hydrogels	923:931	arg1	modulus					904:910	the elastic modulus	892:910	the elastic modulus of TCS/CMC hydrogels with an optimized CMC concentration	892:967	Rheological measurements showed that the elastic modulus of TCS/CMC hydrogels with an optimized CMC concentration could reach around 2.5 kPa or higher than their respective viscous modulus, indicating that they behaved like strong hydrogels.
34428384	3	24	theme	TGF-β1	792:797	arg1	system					758:763	CMC (TCS/CMC) hydrogel system	735:763	CMC (TCS/CMC) hydrogel system	735:763	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	3	24	theme	TGF-β1	792:797	arg1	carriers					799:806	injectable TGF-β1 carriers	781:806	injectable TGF-β1 carriers for cartilage tissue engineering applications	781:852	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	3	25	theme	novel	622:626	arg1	based					647:651	a novel hydrogel composite based	620:651	a novel hydrogel composite based on the polymer	620:666	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	3	25	theme	novel	622:626	arg1	chitosan					692:699	cross-linked thiolated chitosan	669:699	cross-linked thiolated chitosan	669:699	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	0	26	theme	injectable	61:70	arg1	chitosan					95:102	an injectable cross-linked thiolated chitosan	58:102	an injectable cross-linked thiolated chitosan	58:102	Enhanced efficacy of transforming growth factor-β1 loaded an injectable cross-linked thiolated chitosan and carboxymethyl cellulose-based hydrogels for cartilage tissue engineering.
34428384	1	27	theme	Growth	182:187	arg1	GFs					198:200	GFs	198:200	GFs	198:200	Growth factors (GFs) are soluble proteins extracellular that control a wide range of cellular processes as well as tissue regeneration.
34428384	1	27	theme	Growth	182:187	arg1	factors					189:195	Growth factors	182:195	Growth factors (GFs)	182:201	Growth factors (GFs) are soluble proteins extracellular that control a wide range of cellular processes as well as tissue regeneration.
34428384	1	27	theme	Growth	182:187	arg1	proteins					215:222	soluble proteins	207:222	soluble proteins extracellular that control a wide range of cellular processes as well as tissue regeneration	207:315	Growth factors (GFs) are soluble proteins extracellular that control a wide range of cellular processes as well as tissue regeneration.
34428384	9	28	theme	cartilage	1735:1743	arg1	defects					1745:1751	full-thickness cartilage defects	1720:1751	full-thickness cartilage defects	1720:1751	For in vivo cartilage repair, full-thickness cartilage defects were induced on rat knees for 8 weeks.
34428384	3	29	theme	tissue	822:827	arg1	applications					841:852	cartilage tissue engineering applications	812:852	cartilage tissue engineering applications	812:852	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	0	30	theme	thiolated	85:93	arg1	chitosan					95:102	an injectable cross-linked thiolated chitosan	58:102	an injectable cross-linked thiolated chitosan	58:102	Enhanced efficacy of transforming growth factor-β1 loaded an injectable cross-linked thiolated chitosan and carboxymethyl cellulose-based hydrogels for cartilage tissue engineering.
34428384	9	31	theme	rat	1769:1771	arg1	knees					1773:1777	rat knees	1769:1777	rat knees for 8 weeks	1769:1789	For in vivo cartilage repair, full-thickness cartilage defects were induced on rat knees for 8 weeks.
34428384	10	32	theme	novel	1820:1824	arg1	system					1857:1862	this novel TGF-β1-loaded TCS/CMC hydrogel system	1815:1862	this novel TGF-β1-loaded TCS/CMC hydrogel system	1815:1862	The optimal ability of this novel TGF-β1-loaded TCS/CMC hydrogel system was further demonstrated by histological analysis, resulting in a novel therapeutic strategy for repairing articular cartilage defects.
34428384	5	33	theme	TCS/CMC	1284:1290	arg1	hydrogels					1292:1300	the TCS/CMC hydrogels	1280:1300	the TCS/CMC hydrogels	1280:1300	Crosslinking significantly alters the overall network distribution, surface morphology, pore size, porosity, gelation time, swelling ratio, water content, and in vitro degradation of the TCS/CMC hydrogels.
34428384	7	34	theme	TCS/CMC	1555:1561	arg1	hydrogels					1563:1571	the TCS/CMC hydrogels	1551:1571	the TCS/CMC hydrogels	1551:1571	TGF-β1 released marginally from TCS/CMC hydrogels as incubation time increased, up to 21 days, with around 18.6 ± 0.9% of the drug stored inside the TCS/CMC hydrogels.
34428384	0	35	theme	cellulose-based	122:136	arg1	hydrogels					138:146	an injectable cross-linked thiolated chitosan and carboxymethyl cellulose-based hydrogels	58:146	an injectable cross-linked thiolated chitosan and carboxymethyl cellulose-based hydrogels for cartilage tissue engineering	58:179	Enhanced efficacy of transforming growth factor-β1 loaded an injectable cross-linked thiolated chitosan and carboxymethyl cellulose-based hydrogels for cartilage tissue engineering.
34428384	5	36	theme	network	1143:1149	arg1	distribution					1151:1162	overall network distribution	1135:1162	overall network distribution	1135:1162	Crosslinking significantly alters the overall network distribution, surface morphology, pore size, porosity, gelation time, swelling ratio, water content, and in vitro degradation of the TCS/CMC hydrogels.
34428384	0	37	theme	Enhanced	0:7	arg1	efficacy					9:16	Enhanced efficacy	0:16	Enhanced efficacy of transforming growth factor-β1	0:49	Enhanced efficacy of transforming growth factor-β1 loaded an injectable cross-linked thiolated chitosan and carboxymethyl cellulose-based hydrogels for cartilage tissue engineering.
34428384	4	38	with	hydrogels	923:931	arg1	concentration					955:967	an optimized CMC concentration	938:967	an optimized CMC concentration	938:967	Rheological measurements showed that the elastic modulus of TCS/CMC hydrogels with an optimized CMC concentration could reach around 2.5 kPa or higher than their respective viscous modulus, indicating that they behaved like strong hydrogels.
34428384	2	39	theme	protein	439:445	arg1	instability					447:457	its potential protein instability	425:457	its potential protein instability	425:457	While transforming growth factor beta-1 (TGF-β1) promotes chondrogenesis, its medical use is restricted by its potential protein instability, which necessitates high doses of the protein, which can result in adverse side effects such as inefficient cartilage formation.
34428384	4	40	theme	respective	1017:1026	arg1	viscous					1028:1034	their respective viscous	1011:1034	their respective viscous	1011:1034	Rheological measurements showed that the elastic modulus of TCS/CMC hydrogels with an optimized CMC concentration could reach around 2.5 kPa or higher than their respective viscous modulus, indicating that they behaved like strong hydrogels.
34428384	10	41	theme	system	1857:1862	arg1	ability					1804:1810	The optimal ability	1792:1810	The optimal ability of this novel TGF-β1-loaded TCS/CMC hydrogel system	1792:1862	The optimal ability of this novel TGF-β1-loaded TCS/CMC hydrogel system was further demonstrated by histological analysis, resulting in a novel therapeutic strategy for repairing articular cartilage defects.
34428384	0	42	theme	growth	34:39	arg1	factor-β1					41:49	transforming growth factor-β1	21:49	transforming growth factor-β1	21:49	Enhanced efficacy of transforming growth factor-β1 loaded an injectable cross-linked thiolated chitosan and carboxymethyl cellulose-based hydrogels for cartilage tissue engineering.
34428384	5	43	theme	pore	1185:1188	arg1	size					1190:1193	pore size	1185:1193	pore size	1185:1193	Crosslinking significantly alters the overall network distribution, surface morphology, pore size, porosity, gelation time, swelling ratio, water content, and in vitro degradation of the TCS/CMC hydrogels.
34428384	5	44	dep	distribution	1151:1162	arg1	the					1131:1133	the	1131:1133	the	1131:1133	Crosslinking significantly alters the overall network distribution, surface morphology, pore size, porosity, gelation time, swelling ratio, water content, and in vitro degradation of the TCS/CMC hydrogels.
34428384	3	45	theme	composite	637:645	arg1	based					647:651	a novel hydrogel composite based	620:651	a novel hydrogel composite based on the polymer	620:666	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	3	45	theme	composite	637:645	arg1	chitosan					692:699	cross-linked thiolated chitosan	669:699	cross-linked thiolated chitosan	669:699	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	0	46	link	cross-linked	72:83	arg1	chitosan					95:102	an injectable cross-linked thiolated chitosan	58:102	an injectable cross-linked thiolated chitosan	58:102	Enhanced efficacy of transforming growth factor-β1 loaded an injectable cross-linked thiolated chitosan and carboxymethyl cellulose-based hydrogels for cartilage tissue engineering.
34428384	10	47	theme	hydrogel	1848:1855	arg1	system					1857:1862	this novel TGF-β1-loaded TCS/CMC hydrogel system	1815:1862	this novel TGF-β1-loaded TCS/CMC hydrogel system	1815:1862	The optimal ability of this novel TGF-β1-loaded TCS/CMC hydrogel system was further demonstrated by histological analysis, resulting in a novel therapeutic strategy for repairing articular cartilage defects.
34428384	3	48	theme	cross-linked	669:680	arg1	based					647:651	a novel hydrogel composite based	620:651	a novel hydrogel composite based on the polymer	620:666	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	3	48	theme	cross-linked	669:680	arg1	chitosan					692:699	cross-linked thiolated chitosan	669:699	cross-linked thiolated chitosan	669:699	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	8	49	theme	TCS/CMC	1637:1643	arg1	hydrogels					1645:1653	TGF-β1-loaded TCS/CMC hydrogels	1623:1653	TGF-β1-loaded TCS/CMC hydrogels	1623:1653	On day 21, BMSC treated with TGF-β1 in medium or TGF-β1-loaded TCS/CMC hydrogels grew faster than the other groups.
34428384	3	50	dep	based	647:651	arg1	TCS					702:704	TCS	702:704	TCS	702:704	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	3	50	dep	based	647:651	arg1	cellulose					724:732	carboxymethyl cellulose	710:732	carboxymethyl cellulose	710:732	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	3	50	dep	based	647:651	arg1	utilized					769:776	utilized	769:776	was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications	765:852	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	1	51	theme	cellular	267:274	arg1	processes					276:284	cellular processes	267:284	cellular processes	267:284	Growth factors (GFs) are soluble proteins extracellular that control a wide range of cellular processes as well as tissue regeneration.
34428384	10	52	theme	TCS/CMC	1840:1846	arg1	system					1857:1862	this novel TGF-β1-loaded TCS/CMC hydrogel system	1815:1862	this novel TGF-β1-loaded TCS/CMC hydrogel system	1815:1862	The optimal ability of this novel TGF-β1-loaded TCS/CMC hydrogel system was further demonstrated by histological analysis, resulting in a novel therapeutic strategy for repairing articular cartilage defects.
34428384	8	53	theme	other	1676:1680	arg1	groups					1682:1687	the other groups	1672:1687	the other groups	1672:1687	On day 21, BMSC treated with TGF-β1 in medium or TGF-β1-loaded TCS/CMC hydrogels grew faster than the other groups.
34428384	7	54	theme	incubation	1459:1468	arg1	time					1470:1473	incubation time	1459:1473	incubation time	1459:1473	TGF-β1 released marginally from TCS/CMC hydrogels as incubation time increased, up to 21 days, with around 18.6 ± 0.9% of the drug stored inside the TCS/CMC hydrogels.
34428384	3	55	from	based	647:651	arg1	polymer					660:666	the polymer	656:666	the polymer	656:666	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	2	56	theme	high	479:482	arg1	doses					484:488	high doses	479:488	high doses of the protein, which can result in adverse side effects such as inefficient cartilage formation	479:585	While transforming growth factor beta-1 (TGF-β1) promotes chondrogenesis, its medical use is restricted by its potential protein instability, which necessitates high doses of the protein, which can result in adverse side effects such as inefficient cartilage formation.
34428384	3	57	theme	carboxymethyl	710:722	arg1	cellulose					724:732	carboxymethyl cellulose	710:732	carboxymethyl cellulose	710:732	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	10	58	theme	cartilage	1981:1989	arg1	defects					1991:1997	articular cartilage defects	1971:1997	articular cartilage defects	1971:1997	The optimal ability of this novel TGF-β1-loaded TCS/CMC hydrogel system was further demonstrated by histological analysis, resulting in a novel therapeutic strategy for repairing articular cartilage defects.
34428384	2	59	theme	factor	344:349	arg1	TGF-β1					359:364	TGF-β1	359:364	TGF-β1	359:364	While transforming growth factor beta-1 (TGF-β1) promotes chondrogenesis, its medical use is restricted by its potential protein instability, which necessitates high doses of the protein, which can result in adverse side effects such as inefficient cartilage formation.
34428384	2	59	theme	factor	344:349	arg1	beta-1					351:356	transforming growth factor beta-1	324:356	transforming growth factor beta-1 (TGF-β1)	324:365	While transforming growth factor beta-1 (TGF-β1) promotes chondrogenesis, its medical use is restricted by its potential protein instability, which necessitates high doses of the protein, which can result in adverse side effects such as inefficient cartilage formation.
34428384	4	60	theme	Rheological	855:865	arg1	measurements					867:878	Rheological measurements	855:878	Rheological measurements	855:878	Rheological measurements showed that the elastic modulus of TCS/CMC hydrogels with an optimized CMC concentration could reach around 2.5 kPa or higher than their respective viscous modulus, indicating that they behaved like strong hydrogels.
34428384	10	61	theme	articular	1971:1979	arg1	defects					1991:1997	articular cartilage defects	1971:1997	articular cartilage defects	1971:1997	The optimal ability of this novel TGF-β1-loaded TCS/CMC hydrogel system was further demonstrated by histological analysis, resulting in a novel therapeutic strategy for repairing articular cartilage defects.
34428384	0	62	theme	cartilage	152:160	arg1	engineering					169:179	cartilage tissue engineering	152:179	cartilage tissue engineering	152:179	Enhanced efficacy of transforming growth factor-β1 loaded an injectable cross-linked thiolated chitosan and carboxymethyl cellulose-based hydrogels for cartilage tissue engineering.
34428384	2	63	theme	cartilage	567:575	arg1	formation					577:585	inefficient cartilage formation	555:585	inefficient cartilage formation	555:585	While transforming growth factor beta-1 (TGF-β1) promotes chondrogenesis, its medical use is restricted by its potential protein instability, which necessitates high doses of the protein, which can result in adverse side effects such as inefficient cartilage formation.
34428384	2	64	theme	transforming	324:335	arg1	TGF-β1					359:364	TGF-β1	359:364	TGF-β1	359:364	While transforming growth factor beta-1 (TGF-β1) promotes chondrogenesis, its medical use is restricted by its potential protein instability, which necessitates high doses of the protein, which can result in adverse side effects such as inefficient cartilage formation.
34428384	2	64	theme	transforming	324:335	arg1	beta-1					351:356	transforming growth factor beta-1	324:356	transforming growth factor beta-1 (TGF-β1)	324:365	While transforming growth factor beta-1 (TGF-β1) promotes chondrogenesis, its medical use is restricted by its potential protein instability, which necessitates high doses of the protein, which can result in adverse side effects such as inefficient cartilage formation.
34428384	9	65	theme	cartilage	1702:1710	arg1	repair					1712:1717	in vivo cartilage repair	1694:1717	in vivo cartilage repair	1694:1717	For in vivo cartilage repair, full-thickness cartilage defects were induced on rat knees for 8 weeks.
34428384	2	66	theme	side	534:537	arg1	formation					577:585	inefficient cartilage formation	555:585	inefficient cartilage formation	555:585	While transforming growth factor beta-1 (TGF-β1) promotes chondrogenesis, its medical use is restricted by its potential protein instability, which necessitates high doses of the protein, which can result in adverse side effects such as inefficient cartilage formation.
34428384	2	66	theme	side	534:537	arg1	effects					539:545	adverse side effects	526:545	adverse side effects such as inefficient cartilage formation	526:585	While transforming growth factor beta-1 (TGF-β1) promotes chondrogenesis, its medical use is restricted by its potential protein instability, which necessitates high doses of the protein, which can result in adverse side effects such as inefficient cartilage formation.
34428384	6	67	theme	original	1377:1384	arg1	form					1386:1389	their original form	1371:1389	their original form after 21 days	1371:1403	TCS/CMC hydrogels maintain more than 90% of their weight and retain their original form after 21 days.
34428384	6	68	theme	weight	1353:1358	arg1	%					1342:1342	more than 90%	1330:1342	more than 90% of their weight	1330:1358	TCS/CMC hydrogels maintain more than 90% of their weight and retain their original form after 21 days.
34428384	6	68	theme	weight	1353:1358	arg1	weight					1353:1358	their weight	1347:1358	their weight	1347:1358	TCS/CMC hydrogels maintain more than 90% of their weight and retain their original form after 21 days.
34428384	5	69	theme	hydrogels	1292:1300	arg1	degradation					1265:1275	in vitro degradation	1256:1275	in vitro degradation	1256:1275	Crosslinking significantly alters the overall network distribution, surface morphology, pore size, porosity, gelation time, swelling ratio, water content, and in vitro degradation of the TCS/CMC hydrogels.
34428384	5	69	theme	hydrogels	1292:1300	arg1	morphology					1173:1182	surface morphology	1165:1182	surface morphology	1165:1182	Crosslinking significantly alters the overall network distribution, surface morphology, pore size, porosity, gelation time, swelling ratio, water content, and in vitro degradation of the TCS/CMC hydrogels.
34428384	5	69	theme	hydrogels	1292:1300	arg1	time					1215:1218	gelation time	1206:1218	gelation time	1206:1218	Crosslinking significantly alters the overall network distribution, surface morphology, pore size, porosity, gelation time, swelling ratio, water content, and in vitro degradation of the TCS/CMC hydrogels.
34428384	5	69	theme	hydrogels	1292:1300	arg1	content					1243:1249	water content	1237:1249	water content	1237:1249	Crosslinking significantly alters the overall network distribution, surface morphology, pore size, porosity, gelation time, swelling ratio, water content, and in vitro degradation of the TCS/CMC hydrogels.
34428384	5	69	theme	hydrogels	1292:1300	arg1	distribution					1151:1162	overall network distribution	1135:1162	overall network distribution	1135:1162	Crosslinking significantly alters the overall network distribution, surface morphology, pore size, porosity, gelation time, swelling ratio, water content, and in vitro degradation of the TCS/CMC hydrogels.
34428384	5	69	theme	hydrogels	1292:1300	arg1	size					1190:1193	pore size	1185:1193	pore size	1185:1193	Crosslinking significantly alters the overall network distribution, surface morphology, pore size, porosity, gelation time, swelling ratio, water content, and in vitro degradation of the TCS/CMC hydrogels.
34428384	5	69	theme	hydrogels	1292:1300	arg1	porosity					1196:1203	porosity	1196:1203	porosity	1196:1203	Crosslinking significantly alters the overall network distribution, surface morphology, pore size, porosity, gelation time, swelling ratio, water content, and in vitro degradation of the TCS/CMC hydrogels.
34428384	5	69	theme	hydrogels	1292:1300	arg1	ratio					1230:1234	swelling ratio	1221:1234	swelling ratio	1221:1234	Crosslinking significantly alters the overall network distribution, surface morphology, pore size, porosity, gelation time, swelling ratio, water content, and in vitro degradation of the TCS/CMC hydrogels.
34428384	10	70	theme	TGF-β1-loaded	1826:1838	arg1	system					1857:1862	this novel TGF-β1-loaded TCS/CMC hydrogel system	1815:1862	this novel TGF-β1-loaded TCS/CMC hydrogel system	1815:1862	The optimal ability of this novel TGF-β1-loaded TCS/CMC hydrogel system was further demonstrated by histological analysis, resulting in a novel therapeutic strategy for repairing articular cartilage defects.
34428384	4	71	theme	TCS/CMC	915:921	arg1	hydrogels					923:931	TCS/CMC hydrogels	915:931	TCS/CMC hydrogels with an optimized CMC concentration	915:967	Rheological measurements showed that the elastic modulus of TCS/CMC hydrogels with an optimized CMC concentration could reach around 2.5 kPa or higher than their respective viscous modulus, indicating that they behaved like strong hydrogels.
34428384	3	72	used	utilized	769:776	arg2	system					758:763	CMC (TCS/CMC) hydrogel system	735:763	CMC (TCS/CMC) hydrogel system	735:763	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	3	72	used	utilized	769:776	arg2	carriers					799:806	injectable TGF-β1 carriers	781:806	injectable TGF-β1 carriers for cartilage tissue engineering applications	781:852	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	0	73	theme	cross-linked	72:83	arg1	chitosan					95:102	an injectable cross-linked thiolated chitosan	58:102	an injectable cross-linked thiolated chitosan	58:102	Enhanced efficacy of transforming growth factor-β1 loaded an injectable cross-linked thiolated chitosan and carboxymethyl cellulose-based hydrogels for cartilage tissue engineering.
34428384	3	74	theme	injectable	781:790	arg1	system					758:763	CMC (TCS/CMC) hydrogel system	735:763	CMC (TCS/CMC) hydrogel system	735:763	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	3	74	theme	injectable	781:790	arg1	carriers					799:806	injectable TGF-β1 carriers	781:806	injectable TGF-β1 carriers for cartilage tissue engineering applications	781:852	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	0	75	theme	chitosan	95:102	arg1	hydrogels					138:146	an injectable cross-linked thiolated chitosan and carboxymethyl cellulose-based hydrogels	58:146	an injectable cross-linked thiolated chitosan and carboxymethyl cellulose-based hydrogels for cartilage tissue engineering	58:179	Enhanced efficacy of transforming growth factor-β1 loaded an injectable cross-linked thiolated chitosan and carboxymethyl cellulose-based hydrogels for cartilage tissue engineering.
34428384	5	76	theme	surface	1165:1171	arg1	morphology					1173:1182	surface morphology	1165:1182	surface morphology	1165:1182	Crosslinking significantly alters the overall network distribution, surface morphology, pore size, porosity, gelation time, swelling ratio, water content, and in vitro degradation of the TCS/CMC hydrogels.
34428384	4	77	theme	higher	999:1004	arg1	modulus					1036:1042	higher than their respective viscous modulus	999:1042	higher than their respective viscous modulus	999:1042	Rheological measurements showed that the elastic modulus of TCS/CMC hydrogels with an optimized CMC concentration could reach around 2.5 kPa or higher than their respective viscous modulus, indicating that they behaved like strong hydrogels.
34428384	3	78	theme	engineering	829:839	arg1	applications					841:852	cartilage tissue engineering applications	812:852	cartilage tissue engineering applications	812:852	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	0	79	theme	carboxymethyl	108:120	arg1	hydrogels					138:146	an injectable cross-linked thiolated chitosan and carboxymethyl cellulose-based hydrogels	58:146	an injectable cross-linked thiolated chitosan and carboxymethyl cellulose-based hydrogels for cartilage tissue engineering	58:179	Enhanced efficacy of transforming growth factor-β1 loaded an injectable cross-linked thiolated chitosan and carboxymethyl cellulose-based hydrogels for cartilage tissue engineering.
34428384	2	80	theme	adverse	526:532	arg1	formation					577:585	inefficient cartilage formation	555:585	inefficient cartilage formation	555:585	While transforming growth factor beta-1 (TGF-β1) promotes chondrogenesis, its medical use is restricted by its potential protein instability, which necessitates high doses of the protein, which can result in adverse side effects such as inefficient cartilage formation.
34428384	2	80	theme	adverse	526:532	arg1	effects					539:545	adverse side effects	526:545	adverse side effects such as inefficient cartilage formation	526:585	While transforming growth factor beta-1 (TGF-β1) promotes chondrogenesis, its medical use is restricted by its potential protein instability, which necessitates high doses of the protein, which can result in adverse side effects such as inefficient cartilage formation.
34428384	3	81	theme	cartilage	812:820	arg1	applications					841:852	cartilage tissue engineering applications	812:852	cartilage tissue engineering applications	812:852	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	5	82	theme	overall	1135:1141	arg1	distribution					1151:1162	overall network distribution	1135:1162	overall network distribution	1135:1162	Crosslinking significantly alters the overall network distribution, surface morphology, pore size, porosity, gelation time, swelling ratio, water content, and in vitro degradation of the TCS/CMC hydrogels.
34428384	2	83	theme	protein	497:503	arg1	doses					484:488	high doses	479:488	high doses of the protein, which can result in adverse side effects such as inefficient cartilage formation	479:585	While transforming growth factor beta-1 (TGF-β1) promotes chondrogenesis, its medical use is restricted by its potential protein instability, which necessitates high doses of the protein, which can result in adverse side effects such as inefficient cartilage formation.
34428384	4	84	theme	CMC	951:953	arg1	concentration					955:967	an optimized CMC concentration	938:967	an optimized CMC concentration	938:967	Rheological measurements showed that the elastic modulus of TCS/CMC hydrogels with an optimized CMC concentration could reach around 2.5 kPa or higher than their respective viscous modulus, indicating that they behaved like strong hydrogels.
34428384	10	85	dep	novel	1930:1934	arg1	therapeutic					1936:1946	therapeutic	1936:1946	therapeutic	1936:1946	The optimal ability of this novel TGF-β1-loaded TCS/CMC hydrogel system was further demonstrated by histological analysis, resulting in a novel therapeutic strategy for repairing articular cartilage defects.
34428384	2	86	theme	medical	396:402	arg1	use					404:406	its medical use	392:406	its medical use	392:406	While transforming growth factor beta-1 (TGF-β1) promotes chondrogenesis, its medical use is restricted by its potential protein instability, which necessitates high doses of the protein, which can result in adverse side effects such as inefficient cartilage formation.
34428384	0	87	theme	transforming	21:32	arg1	factor-β1					41:49	transforming growth factor-β1	21:49	transforming growth factor-β1	21:49	Enhanced efficacy of transforming growth factor-β1 loaded an injectable cross-linked thiolated chitosan and carboxymethyl cellulose-based hydrogels for cartilage tissue engineering.
34428384	5	88	theme	gelation	1206:1213	arg1	time					1215:1218	gelation time	1206:1218	gelation time	1206:1218	Crosslinking significantly alters the overall network distribution, surface morphology, pore size, porosity, gelation time, swelling ratio, water content, and in vitro degradation of the TCS/CMC hydrogels.
34428384	2	89	theme	potential	429:437	arg1	instability					447:457	its potential protein instability	425:457	its potential protein instability	425:457	While transforming growth factor beta-1 (TGF-β1) promotes chondrogenesis, its medical use is restricted by its potential protein instability, which necessitates high doses of the protein, which can result in adverse side effects such as inefficient cartilage formation.
34428384	0	90	theme	factor-β1	41:49	arg1	efficacy					9:16	Enhanced efficacy	0:16	Enhanced efficacy of transforming growth factor-β1	0:49	Enhanced efficacy of transforming growth factor-β1 loaded an injectable cross-linked thiolated chitosan and carboxymethyl cellulose-based hydrogels for cartilage tissue engineering.
34428384	3	91	theme	hydrogel	628:635	arg1	based					647:651	a novel hydrogel composite based	620:651	a novel hydrogel composite based on the polymer	620:666	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	3	91	theme	hydrogel	628:635	arg1	chitosan					692:699	cross-linked thiolated chitosan	669:699	cross-linked thiolated chitosan	669:699	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	7	92	theme	drug	1532:1535	arg1	%					1523:1523	around 18.6 ± 0.9%	1506:1523	around 18.6 ± 0.9% of the drug stored inside the TCS/CMC hydrogels	1506:1571	TGF-β1 released marginally from TCS/CMC hydrogels as incubation time increased, up to 21 days, with around 18.6 ± 0.9% of the drug stored inside the TCS/CMC hydrogels.
34428384	7	92	theme	drug	1532:1535	arg1	drug					1532:1535	the drug	1528:1535	the drug stored inside the TCS/CMC hydrogels	1528:1571	TGF-β1 released marginally from TCS/CMC hydrogels as incubation time increased, up to 21 days, with around 18.6 ± 0.9% of the drug stored inside the TCS/CMC hydrogels.
34428384	4	93	theme	optimized	941:949	arg1	concentration					955:967	an optimized CMC concentration	938:967	an optimized CMC concentration	938:967	Rheological measurements showed that the elastic modulus of TCS/CMC hydrogels with an optimized CMC concentration could reach around 2.5 kPa or higher than their respective viscous modulus, indicating that they behaved like strong hydrogels.
34428384	10	94	theme	histological	1892:1903	arg1	analysis					1905:1912	histological analysis	1892:1912	histological analysis	1892:1912	The optimal ability of this novel TGF-β1-loaded TCS/CMC hydrogel system was further demonstrated by histological analysis, resulting in a novel therapeutic strategy for repairing articular cartilage defects.
34428384	1	95	theme	wide	253:256	arg1	range					258:262	a wide range	251:262	a wide range of cellular processes as well as tissue regeneration	251:315	Growth factors (GFs) are soluble proteins extracellular that control a wide range of cellular processes as well as tissue regeneration.
34428384	3	96	link	cross-linked	669:680	arg1	based					647:651	a novel hydrogel composite based	620:651	a novel hydrogel composite based on the polymer	620:666	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	3	96	link	cross-linked	669:680	arg1	chitosan					692:699	cross-linked thiolated chitosan	669:699	cross-linked thiolated chitosan	669:699	In this work, we have developed a novel hydrogel composite based on the polymer, cross-linked thiolated chitosan; TCS and carboxymethyl cellulose; CMC (TCS/CMC) hydrogel system was utilized as injectable TGF-β1 carriers for cartilage tissue engineering applications.
34428384	5	97	theme	in vitro	1256:1263	arg1	degradation					1265:1275	in vitro degradation	1256:1275	in vitro degradation	1256:1275	Crosslinking significantly alters the overall network distribution, surface morphology, pore size, porosity, gelation time, swelling ratio, water content, and in vitro degradation of the TCS/CMC hydrogels.
32229207	0	0	theme	drug	113:116	arg1	release					118:124	intelligent drug release	101:124	intelligent drug release	101:124	Double stimuli-responsive cellulose nanocrystals reinforced electrospun PHBV composites membrane for intelligent drug release.
32229207	1	1	theme	ethyl	212:216	arg1	vehicles					375:382	drug delivery vehicles	361:382	drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug	361:436	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	1	theme	ethyl	212:216	arg1	membranes					334:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	0	2	theme	intelligent	101:111	arg1	release					118:124	intelligent drug release	101:124	intelligent drug release	101:124	Double stimuli-responsive cellulose nanocrystals reinforced electrospun PHBV composites membrane for intelligent drug release.
32229207	5	3	theme	mathematical	1011:1022	arg1	models					1024:1029	Korsmeyere-Peppas mathematical models	993:1029	Korsmeyere-Peppas mathematical models	993:1029	The releasing mechanism of composite membranes based on zero order, first order, Higuchi and Korsmeyere-Peppas mathematical models was clearly demonstrated, giving effective technical guidance for practical drug delivery systems.
32229207	1	4	theme	methacrylate	218:229	arg1	vehicles					375:382	drug delivery vehicles	361:382	drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug	361:436	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	4	theme	methacrylate	218:229	arg1	membranes					334:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	5	theme	electrospun	312:322	arg1	vehicles					375:382	drug delivery vehicles	361:382	drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug	361:436	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	5	theme	electrospun	312:322	arg1	membranes					334:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	3	6	from	improvements	577:588	arg1	Tmax					683:686	Tmax	683:686	Tmax	683:686	Moreover, great improvements in fiber diameter uniformity, crystallization ability and maximum decomposition temperature (Tmax) could be achieved at 6 wt% CNC-g-PDMAEMA.
32229207	3	6	from	improvements	577:588	arg1	temperature					670:680	maximum decomposition temperature	648:680	maximum decomposition temperature (Tmax)	648:687	Moreover, great improvements in fiber diameter uniformity, crystallization ability and maximum decomposition temperature (Tmax) could be achieved at 6 wt% CNC-g-PDMAEMA.
32229207	3	6	from	improvements	577:588	arg1	uniformity					608:617	fiber diameter uniformity	593:617	fiber diameter uniformity	593:617	Moreover, great improvements in fiber diameter uniformity, crystallization ability and maximum decomposition temperature (Tmax) could be achieved at 6 wt% CNC-g-PDMAEMA.
32229207	3	6	from	improvements	577:588	arg1	ability					636:642	crystallization ability	620:642	crystallization ability	620:642	Moreover, great improvements in fiber diameter uniformity, crystallization ability and maximum decomposition temperature (Tmax) could be achieved at 6 wt% CNC-g-PDMAEMA.
32229207	5	7	theme	Korsmeyere-Peppas	993:1009	arg1	models					1024:1029	Korsmeyere-Peppas mathematical models	993:1029	Korsmeyere-Peppas mathematical models	993:1029	The releasing mechanism of composite membranes based on zero order, first order, Higuchi and Korsmeyere-Peppas mathematical models was clearly demonstrated, giving effective technical guidance for practical drug delivery systems.
32229207	4	8	dep	intelligent	805:815	arg1	sustained					831:839	sustained	831:839	sustained	831:839	Furthermore, by introducing stimuli-responsive CNC-g-PDMAEMA nanofillers, intelligent and long-term sustained release behavior of composite membranes could be achieved.
32229207	1	9	theme	composite	324:332	arg1	vehicles					375:382	drug delivery vehicles	361:382	drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug	361:436	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	9	theme	composite	324:332	arg1	membranes					334:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	5	10	theme	composite	927:935	arg1	membranes					937:945	composite membranes	927:945	composite membranes based on zero order, first order, Higuchi and Korsmeyere-Peppas mathematical models	927:1029	The releasing mechanism of composite membranes based on zero order, first order, Higuchi and Korsmeyere-Peppas mathematical models was clearly demonstrated, giving effective technical guidance for practical drug delivery systems.
32229207	4	11	theme	membranes	871:879	arg1	behavior					849:856	intelligent and long-term sustained release behavior	805:856	intelligent and long-term sustained release behavior of composite membranes	805:879	Furthermore, by introducing stimuli-responsive CNC-g-PDMAEMA nanofillers, intelligent and long-term sustained release behavior of composite membranes could be achieved.
32229207	3	12	theme	crystallization	620:634	arg1	ability					636:642	crystallization ability	620:642	crystallization ability	620:642	Moreover, great improvements in fiber diameter uniformity, crystallization ability and maximum decomposition temperature (Tmax) could be achieved at 6 wt% CNC-g-PDMAEMA.
32229207	4	13	theme	long-term	821:829	arg1	behavior					849:856	intelligent and long-term sustained release behavior	805:856	intelligent and long-term sustained release behavior of composite membranes	805:879	Furthermore, by introducing stimuli-responsive CNC-g-PDMAEMA nanofillers, intelligent and long-term sustained release behavior of composite membranes could be achieved.
32229207	5	14	theme	effective	1064:1072	arg1	guidance					1084:1091	effective technical guidance	1064:1091	effective technical guidance for practical drug delivery systems	1064:1127	The releasing mechanism of composite membranes based on zero order, first order, Higuchi and Korsmeyere-Peppas mathematical models was clearly demonstrated, giving effective technical guidance for practical drug delivery systems.
32229207	2	15	theme	CNC-g-PDMAEMA	463:475	arg1	nanoparticles					477:489	rigid CNC-g-PDMAEMA nanoparticles	457:489	rigid CNC-g-PDMAEMA nanoparticles	457:489	It was found that rigid CNC-g-PDMAEMA nanoparticles enhanced thermal, crystallization and hydrophilic properties of PHBV.
32229207	3	16	theme	diameter	599:606	arg1	uniformity					608:617	fiber diameter uniformity	593:617	fiber diameter uniformity	593:617	Moreover, great improvements in fiber diameter uniformity, crystallization ability and maximum decomposition temperature (Tmax) could be achieved at 6 wt% CNC-g-PDMAEMA.
32229207	5	17	theme	first	968:972	arg1	order					974:978	first order	968:978	first order	968:978	The releasing mechanism of composite membranes based on zero order, first order, Higuchi and Korsmeyere-Peppas mathematical models was clearly demonstrated, giving effective technical guidance for practical drug delivery systems.
32229207	1	18	theme	model	427:431	arg1	hydrochloride					403:415	tetracycline hydrochloride	390:415	tetracycline hydrochloride (TH)	390:420	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	18	theme	model	427:431	arg1	drug					433:436	a model drug	425:436	a model drug	425:436	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	5	19	theme	delivery	1112:1119	arg1	systems					1121:1127	practical drug delivery systems	1097:1127	practical drug delivery systems	1097:1127	The releasing mechanism of composite membranes based on zero order, first order, Higuchi and Korsmeyere-Peppas mathematical models was clearly demonstrated, giving effective technical guidance for practical drug delivery systems.
32229207	0	20	theme	stimuli-responsive	7:24	arg1	nanocrystals					36:47	Double stimuli-responsive cellulose nanocrystals	0:47	Double stimuli-responsive cellulose nanocrystals	0:47	Double stimuli-responsive cellulose nanocrystals reinforced electrospun PHBV composites membrane for intelligent drug release.
32229207	2	21	theme	PHBV	555:558	arg1	properties					541:550	thermal, crystallization and hydrophilic properties	500:550	thermal, crystallization and hydrophilic properties of PHBV	500:558	It was found that rigid CNC-g-PDMAEMA nanoparticles enhanced thermal, crystallization and hydrophilic properties of PHBV.
32229207	3	22	theme	maximum	648:654	arg1	Tmax					683:686	Tmax	683:686	Tmax	683:686	Moreover, great improvements in fiber diameter uniformity, crystallization ability and maximum decomposition temperature (Tmax) could be achieved at 6 wt% CNC-g-PDMAEMA.
32229207	3	22	theme	maximum	648:654	arg1	temperature					670:680	maximum decomposition temperature	648:680	maximum decomposition temperature (Tmax)	648:687	Moreover, great improvements in fiber diameter uniformity, crystallization ability and maximum decomposition temperature (Tmax) could be achieved at 6 wt% CNC-g-PDMAEMA.
32229207	1	23	theme	CNC-g-PDMAEMA	233:245	arg1	vehicles					375:382	drug delivery vehicles	361:382	drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug	361:436	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	23	theme	CNC-g-PDMAEMA	233:245	arg1	membranes					334:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	0	24	theme	Double	0:5	arg1	nanocrystals					36:47	Double stimuli-responsive cellulose nanocrystals	0:47	Double stimuli-responsive cellulose nanocrystals	0:47	Double stimuli-responsive cellulose nanocrystals reinforced electrospun PHBV composites membrane for intelligent drug release.
32229207	3	25	theme	decomposition	656:668	arg1	Tmax					683:686	Tmax	683:686	Tmax	683:686	Moreover, great improvements in fiber diameter uniformity, crystallization ability and maximum decomposition temperature (Tmax) could be achieved at 6 wt% CNC-g-PDMAEMA.
32229207	3	25	theme	decomposition	656:668	arg1	temperature					670:680	maximum decomposition temperature	648:680	maximum decomposition temperature (Tmax)	648:687	Moreover, great improvements in fiber diameter uniformity, crystallization ability and maximum decomposition temperature (Tmax) could be achieved at 6 wt% CNC-g-PDMAEMA.
32229207	2	26	theme	thermal	500:506	arg1	properties					541:550	thermal, crystallization and hydrophilic properties	500:550	thermal, crystallization and hydrophilic properties of PHBV	500:558	It was found that rigid CNC-g-PDMAEMA nanoparticles enhanced thermal, crystallization and hydrophilic properties of PHBV.
32229207	4	27	theme	release	841:847	arg1	behavior					849:856	intelligent and long-term sustained release behavior	805:856	intelligent and long-term sustained release behavior of composite membranes	805:879	Furthermore, by introducing stimuli-responsive CNC-g-PDMAEMA nanofillers, intelligent and long-term sustained release behavior of composite membranes could be achieved.
32229207	1	28	theme	Double	127:132	arg1	vehicles					375:382	drug delivery vehicles	361:382	drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug	361:436	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	28	theme	Double	127:132	arg1	membranes					334:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	29	theme	reinforced	248:257	arg1	vehicles					375:382	drug delivery vehicles	361:382	drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug	361:436	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	29	theme	reinforced	248:257	arg1	membranes					334:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	0	30	theme	cellulose	26:34	arg1	nanocrystals					36:47	Double stimuli-responsive cellulose nanocrystals	0:47	Double stimuli-responsive cellulose nanocrystals	0:47	Double stimuli-responsive cellulose nanocrystals reinforced electrospun PHBV composites membrane for intelligent drug release.
32229207	5	31	theme	drug	1107:1110	arg1	systems					1121:1127	practical drug delivery systems	1097:1127	practical drug delivery systems	1097:1127	The releasing mechanism of composite membranes based on zero order, first order, Higuchi and Korsmeyere-Peppas mathematical models was clearly demonstrated, giving effective technical guidance for practical drug delivery systems.
32229207	5	32	theme	technical	1074:1082	arg1	guidance					1084:1091	effective technical guidance	1064:1091	effective technical guidance for practical drug delivery systems	1064:1127	The releasing mechanism of composite membranes based on zero order, first order, Higuchi and Korsmeyere-Peppas mathematical models was clearly demonstrated, giving effective technical guidance for practical drug delivery systems.
32229207	1	33	theme	stimuli-responsive	134:151	arg1	vehicles					375:382	drug delivery vehicles	361:382	drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug	361:436	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	33	theme	stimuli-responsive	134:151	arg1	membranes					334:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	34	theme	poly	259:262	arg1	vehicles					375:382	drug delivery vehicles	361:382	drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug	361:436	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	34	theme	poly	259:262	arg1	membranes					334:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	0	35	theme	electrospun	60:70	arg1	composites					77:86	electrospun PHBV composites	60:86	electrospun PHBV composites membrane for intelligent drug release	60:124	Double stimuli-responsive cellulose nanocrystals reinforced electrospun PHBV composites membrane for intelligent drug release.
32229207	1	36	theme	drug	361:364	arg1	vehicles					375:382	drug delivery vehicles	361:382	drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug	361:436	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	36	theme	drug	361:364	arg1	membranes					334:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	0	37	dep	composites	77:86	arg1	membrane					88:95	membrane	88:95	membrane	88:95	Double stimuli-responsive cellulose nanocrystals reinforced electrospun PHBV composites membrane for intelligent drug release.
32229207	2	38	theme	rigid	457:461	arg1	nanoparticles					477:489	rigid CNC-g-PDMAEMA nanoparticles	457:489	rigid CNC-g-PDMAEMA nanoparticles	457:489	It was found that rigid CNC-g-PDMAEMA nanoparticles enhanced thermal, crystallization and hydrophilic properties of PHBV.
32229207	4	39	theme	CNC-g-PDMAEMA	778:790	arg1	nanofillers					792:802	stimuli-responsive CNC-g-PDMAEMA nanofillers	759:802	stimuli-responsive CNC-g-PDMAEMA nanofillers	759:802	Furthermore, by introducing stimuli-responsive CNC-g-PDMAEMA nanofillers, intelligent and long-term sustained release behavior of composite membranes could be achieved.
32229207	1	40	theme	delivery	366:373	arg1	vehicles					375:382	drug delivery vehicles	361:382	drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug	361:436	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	40	theme	delivery	366:373	arg1	membranes					334:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	3	41	theme	6 wt	710:713	arg1	CNC-g-PDMAEMA					716:728	6 wt% CNC-g-PDMAEMA	710:728	6 wt% CNC-g-PDMAEMA	710:728	Moreover, great improvements in fiber diameter uniformity, crystallization ability and maximum decomposition temperature (Tmax) could be achieved at 6 wt% CNC-g-PDMAEMA.
32229207	5	42	theme	membranes	937:945	arg1	mechanism					914:922	The releasing mechanism	900:922	The releasing mechanism of composite membranes based on zero order, first order, Higuchi and Korsmeyere-Peppas mathematical models	900:1029	The releasing mechanism of composite membranes based on zero order, first order, Higuchi and Korsmeyere-Peppas mathematical models was clearly demonstrated, giving effective technical guidance for practical drug delivery systems.
32229207	1	43	theme	3-hydroxybutyrate-co-3-hydroxy	264:293	arg1	poly					259:262	poly	259:262	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	43	theme	3-hydroxybutyrate-co-3-hydroxy	264:293	arg1	valerate					295:302	3-hydroxybutyrate-co-3-hydroxy valerate	264:302	3-hydroxybutyrate-co-3-hydroxy valerate	264:302	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	2	44	theme	hydrophilic	529:539	arg1	properties					541:550	thermal, crystallization and hydrophilic properties	500:550	thermal, crystallization and hydrophilic properties of PHBV	500:558	It was found that rigid CNC-g-PDMAEMA nanoparticles enhanced thermal, crystallization and hydrophilic properties of PHBV.
32229207	3	45	theme	great	571:575	arg1	improvements					577:588	great improvements	571:588	great improvements in fiber diameter uniformity, crystallization ability and maximum decomposition temperature (Tmax)	571:687	Moreover, great improvements in fiber diameter uniformity, crystallization ability and maximum decomposition temperature (Tmax) could be achieved at 6 wt% CNC-g-PDMAEMA.
32229207	3	46	theme	%	714:714	arg1	CNC-g-PDMAEMA					716:728	6 wt% CNC-g-PDMAEMA	710:728	6 wt% CNC-g-PDMAEMA	710:728	Moreover, great improvements in fiber diameter uniformity, crystallization ability and maximum decomposition temperature (Tmax) could be achieved at 6 wt% CNC-g-PDMAEMA.
32229207	5	47	theme	practical	1097:1105	arg1	systems					1121:1127	practical drug delivery systems	1097:1127	practical drug delivery systems	1097:1127	The releasing mechanism of composite membranes based on zero order, first order, Higuchi and Korsmeyere-Peppas mathematical models was clearly demonstrated, giving effective technical guidance for practical drug delivery systems.
32229207	1	48	theme	functionalized	153:166	arg1	vehicles					375:382	drug delivery vehicles	361:382	drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug	361:436	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	48	theme	functionalized	153:166	arg1	membranes					334:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	0	49	theme	PHBV	72:75	arg1	composites					77:86	electrospun PHBV composites	60:86	electrospun PHBV composites membrane for intelligent drug release	60:124	Double stimuli-responsive cellulose nanocrystals reinforced electrospun PHBV composites membrane for intelligent drug release.
32229207	1	50	theme	cellulose	168:176	arg1	vehicles					375:382	drug delivery vehicles	361:382	drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug	361:436	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	50	theme	cellulose	168:176	arg1	membranes					334:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	51	theme	tetracycline	390:401	arg1	TH					418:419	TH	418:419	TH	418:419	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	51	theme	tetracycline	390:401	arg1	hydrochloride					403:415	tetracycline hydrochloride	390:415	tetracycline hydrochloride (TH)	390:420	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	51	theme	tetracycline	390:401	arg1	drug					433:436	a model drug	425:436	a model drug	425:436	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	4	52	theme	stimuli-responsive	759:776	arg1	nanofillers					792:802	stimuli-responsive CNC-g-PDMAEMA nanofillers	759:802	stimuli-responsive CNC-g-PDMAEMA nanofillers	759:802	Furthermore, by introducing stimuli-responsive CNC-g-PDMAEMA nanofillers, intelligent and long-term sustained release behavior of composite membranes could be achieved.
32229207	1	53	theme	nanocrystal-poly[2-	178:196	arg1	vehicles					375:382	drug delivery vehicles	361:382	drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug	361:436	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	53	theme	nanocrystal-poly[2-	178:196	arg1	membranes					334:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	4	54	theme	composite	861:869	arg1	membranes					871:879	composite membranes	861:879	composite membranes	861:879	Furthermore, by introducing stimuli-responsive CNC-g-PDMAEMA nanofillers, intelligent and long-term sustained release behavior of composite membranes could be achieved.
32229207	3	55	theme	fiber	593:597	arg1	uniformity					608:617	fiber diameter uniformity	593:617	fiber diameter uniformity	593:617	Moreover, great improvements in fiber diameter uniformity, crystallization ability and maximum decomposition temperature (Tmax) could be achieved at 6 wt% CNC-g-PDMAEMA.
32229207	1	56	theme	dimethylamino	198:210	arg1	vehicles					375:382	drug delivery vehicles	361:382	drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug	361:436	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	1	56	theme	dimethylamino	198:210	arg1	membranes					334:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes	127:342	Double stimuli-responsive functionalized cellulose nanocrystal-poly[2-(dimethylamino)ethyl methacrylate] (CNC-g-PDMAEMA) reinforced poly(3-hydroxybutyrate-co-3-hydroxy valerate) (PHBV) electrospun composite membranes were explored as drug delivery vehicles using tetracycline hydrochloride (TH) as a model drug.
32229207	5	57	theme	releasing	904:912	arg1	mechanism					914:922	The releasing mechanism	900:922	The releasing mechanism of composite membranes based on zero order, first order, Higuchi and Korsmeyere-Peppas mathematical models	900:1029	The releasing mechanism of composite membranes based on zero order, first order, Higuchi and Korsmeyere-Peppas mathematical models was clearly demonstrated, giving effective technical guidance for practical drug delivery systems.
32229207	2	58	theme	crystallization	509:523	arg1	properties					541:550	thermal, crystallization and hydrophilic properties	500:550	thermal, crystallization and hydrophilic properties of PHBV	500:558	It was found that rigid CNC-g-PDMAEMA nanoparticles enhanced thermal, crystallization and hydrophilic properties of PHBV.
32229207	4	59	theme	intelligent	805:815	arg1	behavior					849:856	intelligent and long-term sustained release behavior	805:856	intelligent and long-term sustained release behavior of composite membranes	805:879	Furthermore, by introducing stimuli-responsive CNC-g-PDMAEMA nanofillers, intelligent and long-term sustained release behavior of composite membranes could be achieved.
34051463	6	0	theme	World	1419:1423	arg1	Organization					1432:1443	World Health Organization (WHO)	1419:1449	World Health Organization (WHO)	1419:1449	Furthermore, the PAO/BC aerogels-packed column can continuously and effectively treat the simulated wastewater with multiple heavy metals coexisting to below the threshold value in the drinking water recommended by World Health Organization (WHO), highlighting its feasibility in the complex environmental water.
34051463	6	1	theme	heavy	1329:1333	arg1	metals					1335:1340	multiple heavy metals	1320:1340	multiple heavy metals coexisting to below the threshold value in the drinking water recommended by World Health Organization (WHO)	1320:1449	Furthermore, the PAO/BC aerogels-packed column can continuously and effectively treat the simulated wastewater with multiple heavy metals coexisting to below the threshold value in the drinking water recommended by World Health Organization (WHO), highlighting its feasibility in the complex environmental water.
34051463	4	2	theme	heavy	1036:1040	arg1	metals					1042:1047	five heavy metals	1031:1047	the investigated five heavy metals	1014:1047	Meanwhile, the sorption equilibrium for the investigated five heavy metals is achieved within 25 min with high removal efficiencies.
34051463	6	3	theme	Health	1425:1430	arg1	Organization					1432:1443	World Health Organization (WHO)	1419:1449	World Health Organization (WHO)	1419:1449	Furthermore, the PAO/BC aerogels-packed column can continuously and effectively treat the simulated wastewater with multiple heavy metals coexisting to below the threshold value in the drinking water recommended by World Health Organization (WHO), highlighting its feasibility in the complex environmental water.
34051463	3	4	theme	functional	656:665	arg1	moieties					667:674	amidoxime functional moieties	646:674	amidoxime functional moieties	646:674	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	6	5	theme	multiple	1320:1327	arg1	metals					1335:1340	multiple heavy metals	1320:1340	multiple heavy metals coexisting to below the threshold value in the drinking water recommended by World Health Organization (WHO)	1320:1449	Furthermore, the PAO/BC aerogels-packed column can continuously and effectively treat the simulated wastewater with multiple heavy metals coexisting to below the threshold value in the drinking water recommended by World Health Organization (WHO), highlighting its feasibility in the complex environmental water.
34051463	6	6	theme	drinking	1389:1396	arg1	water					1398:1402	the drinking water	1385:1402	the drinking water recommended by World Health Organization (WHO)	1385:1449	Furthermore, the PAO/BC aerogels-packed column can continuously and effectively treat the simulated wastewater with multiple heavy metals coexisting to below the threshold value in the drinking water recommended by World Health Organization (WHO), highlighting its feasibility in the complex environmental water.
34051463	1	7	theme	efficient	150:158	arg1	adsorbents					174:183	Developing cheap, green, efficient and renewable adsorbents	125:183	Developing cheap, green, efficient and renewable adsorbents	125:183	Developing cheap, green, efficient and renewable adsorbents to address the issue of heavy metal pollution is highly desired for satisfying the requirements of economy sustainability and water security.
34051463	6	8	theme	complex	1488:1494	arg1	water					1510:1514	the complex environmental water	1484:1514	the complex environmental water	1484:1514	Furthermore, the PAO/BC aerogels-packed column can continuously and effectively treat the simulated wastewater with multiple heavy metals coexisting to below the threshold value in the drinking water recommended by World Health Organization (WHO), highlighting its feasibility in the complex environmental water.
34051463	2	9	theme	in	482:483	arg1	transformation					500:513	in situ oximation transformation	482:513	in situ oximation transformation	482:513	Herein, a composite aerogel composed of bacterial cellulose (BC) and poly(amidoxime) (PAO) has been fabricated via a facile and scalable self-assembly and in situ oximation transformation for heavy metals removal.
34051463	3	10	theme	moieties	667:674	arg1	architecture					613:624	unique three-dimensional (3D) interconnected porous architecture	561:624	unique three-dimensional (3D) interconnected porous architecture	561:624	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	3	10	theme	moieties	667:674	arg1	density					635:641	high density	630:641	high density	630:641	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	4	11	with	25 min	1068:1073	arg1	efficiencies					1093:1104	high removal efficiencies	1080:1104	high removal efficiencies	1080:1104	Meanwhile, the sorption equilibrium for the investigated five heavy metals is achieved within 25 min with high removal efficiencies.
34051463	6	12	theme	aerogels-packed	1228:1242	arg1	column					1244:1249	the PAO/BC aerogels-packed column	1217:1249	the PAO/BC aerogels-packed column	1217:1249	Furthermore, the PAO/BC aerogels-packed column can continuously and effectively treat the simulated wastewater with multiple heavy metals coexisting to below the threshold value in the drinking water recommended by World Health Organization (WHO), highlighting its feasibility in the complex environmental water.
34051463	0	13	theme	efficient	87:95	arg1	scavenging					97:106	highly efficient scavenging	80:106	highly efficient scavenging of heavy metals	80:122	Hierarchically porous poly(amidoxime)/bacterial cellulose composite aerogel for highly efficient scavenging of heavy metals.
34051463	3	14	theme	composite	698:706	arg1	capable					719:725	capable	719:725	capable	719:725	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	3	14	theme	composite	698:706	arg1	aerogel					708:714	the developed PAO/BC composite aerogel	677:714	the developed PAO/BC composite aerogel	677:714	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	6	15	theme	threshold	1366:1374	arg1	value					1376:1380	the threshold value	1362:1380	the threshold value in the drinking water recommended by World Health Organization (WHO)	1362:1449	Furthermore, the PAO/BC aerogels-packed column can continuously and effectively treat the simulated wastewater with multiple heavy metals coexisting to below the threshold value in the drinking water recommended by World Health Organization (WHO), highlighting its feasibility in the complex environmental water.
34051463	4	16	theme	sorption	989:996	arg1	equilibrium					998:1008	the sorption equilibrium	985:1008	the sorption equilibrium for the investigated five heavy metals	985:1047	Meanwhile, the sorption equilibrium for the investigated five heavy metals is achieved within 25 min with high removal efficiencies.
34051463	4	16	theme	sorption	989:996	arg1	Meanwhile					974:982	Meanwhile	974:982	Meanwhile	974:982	Meanwhile, the sorption equilibrium for the investigated five heavy metals is achieved within 25 min with high removal efficiencies.
34051463	0	17	theme	heavy	111:115	arg1	metals					117:122	heavy metals	111:122	heavy metals	111:122	Hierarchically porous poly(amidoxime)/bacterial cellulose composite aerogel for highly efficient scavenging of heavy metals.
34051463	3	18	dep	architecture	613:624	arg1	the					557:559	the	557:559	the	557:559	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	6	19	theme	simulated	1294:1302	arg1	wastewater					1304:1313	the simulated wastewater	1290:1313	the simulated wastewater with multiple heavy metals coexisting to below the threshold value in the drinking water recommended by World Health Organization (WHO)	1290:1449	Furthermore, the PAO/BC aerogels-packed column can continuously and effectively treat the simulated wastewater with multiple heavy metals coexisting to below the threshold value in the drinking water recommended by World Health Organization (WHO), highlighting its feasibility in the complex environmental water.
34051463	6	20	theme	environmental	1496:1508	arg1	water					1510:1514	the complex environmental water	1484:1514	the complex environmental water	1484:1514	Furthermore, the PAO/BC aerogels-packed column can continuously and effectively treat the simulated wastewater with multiple heavy metals coexisting to below the threshold value in the drinking water recommended by World Health Organization (WHO), highlighting its feasibility in the complex environmental water.
34051463	2	21	theme	scalable	455:462	arg1	self-assembly					464:476	a facile and scalable self-assembly	442:476	a facile and scalable self-assembly	442:476	Herein, a composite aerogel composed of bacterial cellulose (BC) and poly(amidoxime) (PAO) has been fabricated via a facile and scalable self-assembly and in situ oximation transformation for heavy metals removal.
34051463	3	22	theme	developed	681:689	arg1	capable					719:725	capable	719:725	capable	719:725	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	3	22	theme	developed	681:689	arg1	aerogel					708:714	the developed PAO/BC composite aerogel	677:714	the developed PAO/BC composite aerogel	677:714	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	6	23	from	value	1376:1380	arg1	water					1398:1402	the drinking water	1385:1402	the drinking water recommended by World Health Organization (WHO)	1385:1449	Furthermore, the PAO/BC aerogels-packed column can continuously and effectively treat the simulated wastewater with multiple heavy metals coexisting to below the threshold value in the drinking water recommended by World Health Organization (WHO), highlighting its feasibility in the complex environmental water.
34051463	1	24	theme	renewable	164:172	arg1	adsorbents					174:183	Developing cheap, green, efficient and renewable adsorbents	125:183	Developing cheap, green, efficient and renewable adsorbents	125:183	Developing cheap, green, efficient and renewable adsorbents to address the issue of heavy metal pollution is highly desired for satisfying the requirements of economy sustainability and water security.
34051463	0	25	theme	porous	15:20	arg1	composite					58:66	Hierarchically porous poly(amidoxime)/bacterial cellulose composite	0:66	Hierarchically porous poly(amidoxime)/bacterial cellulose composite	0:66	Hierarchically porous poly(amidoxime)/bacterial cellulose composite aerogel for highly efficient scavenging of heavy metals.
34051463	3	26	theme	PAO/BC	691:696	arg1	capable					719:725	capable	719:725	capable	719:725	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	3	26	theme	PAO/BC	691:696	arg1	aerogel					708:714	the developed PAO/BC composite aerogel	677:714	the developed PAO/BC composite aerogel	677:714	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	6	27	dep	Organization	1432:1443	arg1	WHO					1446:1448	WHO	1446:1448	WHO	1446:1448	Furthermore, the PAO/BC aerogels-packed column can continuously and effectively treat the simulated wastewater with multiple heavy metals coexisting to below the threshold value in the drinking water recommended by World Health Organization (WHO), highlighting its feasibility in the complex environmental water.
34051463	5	28	theme	superior	1170:1177	arg1	performance					1191:1201	superior reusability performance	1170:1201	superior reusability performance	1170:1201	Significantly, the developed PAO/BC composite aerogels possess superior reusability performance.
34051463	2	29	dep	in	482:483	arg1	situ					485:488	situ	485:488	situ	485:488	Herein, a composite aerogel composed of bacterial cellulose (BC) and poly(amidoxime) (PAO) has been fabricated via a facile and scalable self-assembly and in situ oximation transformation for heavy metals removal.
34051463	5	30	theme	reusability	1179:1189	arg1	performance					1191:1201	superior reusability performance	1170:1201	superior reusability performance	1170:1201	Significantly, the developed PAO/BC composite aerogels possess superior reusability performance.
34051463	2	31	theme	bacterial	367:375	arg1	BC					388:389	BC	388:389	BC	388:389	Herein, a composite aerogel composed of bacterial cellulose (BC) and poly(amidoxime) (PAO) has been fabricated via a facile and scalable self-assembly and in situ oximation transformation for heavy metals removal.
34051463	2	31	theme	bacterial	367:375	arg1	cellulose					377:385	bacterial cellulose	367:385	bacterial cellulose (BC)	367:390	Herein, a composite aerogel composed of bacterial cellulose (BC) and poly(amidoxime) (PAO) has been fabricated via a facile and scalable self-assembly and in situ oximation transformation for heavy metals removal.
34051463	4	32	theme	removal	1085:1091	arg1	efficiencies					1093:1104	high removal efficiencies	1080:1104	high removal efficiencies	1080:1104	Meanwhile, the sorption equilibrium for the investigated five heavy metals is achieved within 25 min with high removal efficiencies.
34051463	4	33	theme	investigated	1018:1029	arg1	metals					1042:1047	five heavy metals	1031:1047	the investigated five heavy metals	1014:1047	Meanwhile, the sorption equilibrium for the investigated five heavy metals is achieved within 25 min with high removal efficiencies.
34051463	0	34	theme	metals	117:122	arg1	scavenging					97:106	highly efficient scavenging	80:106	highly efficient scavenging of heavy metals	80:122	Hierarchically porous poly(amidoxime)/bacterial cellulose composite aerogel for highly efficient scavenging of heavy metals.
34051463	2	35	theme	composite	337:345	arg1	aerogel					347:353	a composite aerogel	335:353	a composite aerogel composed of bacterial cellulose (BC) and poly(amidoxime) (PAO)	335:416	Herein, a composite aerogel composed of bacterial cellulose (BC) and poly(amidoxime) (PAO) has been fabricated via a facile and scalable self-assembly and in situ oximation transformation for heavy metals removal.
34051463	5	36	theme	PAO/BC	1136:1141	arg1	aerogels					1153:1160	the developed PAO/BC composite aerogels	1122:1160	the developed PAO/BC composite aerogels	1122:1160	Significantly, the developed PAO/BC composite aerogels possess superior reusability performance.
34051463	1	37	theme	economy	284:290	arg1	sustainability					292:305	economy sustainability	284:305	economy sustainability	284:305	Developing cheap, green, efficient and renewable adsorbents to address the issue of heavy metal pollution is highly desired for satisfying the requirements of economy sustainability and water security.
34051463	2	38	theme	oximation	490:498	arg1	transformation					500:513	in situ oximation transformation	482:513	in situ oximation transformation	482:513	Herein, a composite aerogel composed of bacterial cellulose (BC) and poly(amidoxime) (PAO) has been fabricated via a facile and scalable self-assembly and in situ oximation transformation for heavy metals removal.
34051463	6	39	from	feasibility	1469:1479	arg1	water					1510:1514	the complex environmental water	1484:1514	the complex environmental water	1484:1514	Furthermore, the PAO/BC aerogels-packed column can continuously and effectively treat the simulated wastewater with multiple heavy metals coexisting to below the threshold value in the drinking water recommended by World Health Organization (WHO), highlighting its feasibility in the complex environmental water.
34051463	1	40	theme	sustainability	292:305	arg1	requirements					268:279	the requirements	264:279	the requirements of economy sustainability and water security	264:324	Developing cheap, green, efficient and renewable adsorbents to address the issue of heavy metal pollution is highly desired for satisfying the requirements of economy sustainability and water security.
34051463	3	41	theme	interconnected	591:604	arg1	architecture					613:624	unique three-dimensional (3D) interconnected porous architecture	561:624	unique three-dimensional (3D) interconnected porous architecture	561:624	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	3	42	theme	exceptional	774:784	arg1	capacities					795:804	exceptional sorption capacities	774:804	exceptional sorption capacities	774:804	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	3	43	theme	heavy	756:760	arg1	metals					762:767	heavy metals	756:767	heavy metals	756:767	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	0	44	theme	cellulose	48:56	arg1	composite					58:66	Hierarchically porous poly(amidoxime)/bacterial cellulose composite	0:66	Hierarchically porous poly(amidoxime)/bacterial cellulose composite	0:66	Hierarchically porous poly(amidoxime)/bacterial cellulose composite aerogel for highly efficient scavenging of heavy metals.
34051463	5	45	theme	composite	1143:1151	arg1	aerogels					1153:1160	the developed PAO/BC composite aerogels	1122:1160	the developed PAO/BC composite aerogels	1122:1160	Significantly, the developed PAO/BC composite aerogels possess superior reusability performance.
34051463	3	46	theme	porous	606:611	arg1	architecture					613:624	unique three-dimensional (3D) interconnected porous architecture	561:624	unique three-dimensional (3D) interconnected porous architecture	561:624	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	5	47	theme	developed	1126:1134	arg1	aerogels					1153:1160	the developed PAO/BC composite aerogels	1122:1160	the developed PAO/BC composite aerogels	1122:1160	Significantly, the developed PAO/BC composite aerogels possess superior reusability performance.
34051463	0	48	theme	/bacterial	37:46	arg1	composite					58:66	Hierarchically porous poly(amidoxime)/bacterial cellulose composite	0:66	Hierarchically porous poly(amidoxime)/bacterial cellulose composite	0:66	Hierarchically porous poly(amidoxime)/bacterial cellulose composite aerogel for highly efficient scavenging of heavy metals.
34051463	1	49	theme	water	311:315	arg1	security					317:324	water security	311:324	water security	311:324	Developing cheap, green, efficient and renewable adsorbents to address the issue of heavy metal pollution is highly desired for satisfying the requirements of economy sustainability and water security.
34051463	5	50	contain	possess	1162:1168	arg2	performance					1191:1201	superior reusability performance	1170:1201	superior reusability performance	1170:1201	Significantly, the developed PAO/BC composite aerogels possess superior reusability performance.
34051463	5	50	contain	possess	1162:1168	arg1	aerogels					1153:1160	the developed PAO/BC composite aerogels	1122:1160	the developed PAO/BC composite aerogels	1122:1160	Significantly, the developed PAO/BC composite aerogels possess superior reusability performance.
34051463	3	51	dep	571.5 mg g-1	812:823	arg1	e.g.					807:810	e.g.	807:810	e.g.	807:810	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	1	52	theme	security	317:324	arg1	requirements					268:279	the requirements	264:279	the requirements of economy sustainability and water security	264:324	Developing cheap, green, efficient and renewable adsorbents to address the issue of heavy metal pollution is highly desired for satisfying the requirements of economy sustainability and water security.
34051463	2	53	theme	metals	525:530	arg1	removal					532:538	heavy metals removal	519:538	heavy metals removal	519:538	Herein, a composite aerogel composed of bacterial cellulose (BC) and poly(amidoxime) (PAO) has been fabricated via a facile and scalable self-assembly and in situ oximation transformation for heavy metals removal.
34051463	3	54	theme	amidoxime	646:654	arg1	moieties					667:674	amidoxime functional moieties	646:674	amidoxime functional moieties	646:674	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	3	55	theme	3D	587:588	arg1	architecture					613:624	unique three-dimensional (3D) interconnected porous architecture	561:624	unique three-dimensional (3D) interconnected porous architecture	561:624	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	3	56	theme	unique	561:566	arg1	architecture					613:624	unique three-dimensional (3D) interconnected porous architecture	561:624	unique three-dimensional (3D) interconnected porous architecture	561:624	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	1	57	theme	Developing	125:134	arg1	adsorbents					174:183	Developing cheap, green, efficient and renewable adsorbents	125:183	Developing cheap, green, efficient and renewable adsorbents	125:183	Developing cheap, green, efficient and renewable adsorbents to address the issue of heavy metal pollution is highly desired for satisfying the requirements of economy sustainability and water security.
34051463	6	58	with	wastewater	1304:1313	arg1	metals					1335:1340	multiple heavy metals	1320:1340	multiple heavy metals coexisting to below the threshold value in the drinking water recommended by World Health Organization (WHO)	1320:1449	Furthermore, the PAO/BC aerogels-packed column can continuously and effectively treat the simulated wastewater with multiple heavy metals coexisting to below the threshold value in the drinking water recommended by World Health Organization (WHO), highlighting its feasibility in the complex environmental water.
34051463	1	59	theme	heavy	209:213	arg1	pollution					221:229	heavy metal pollution	209:229	heavy metal pollution	209:229	Developing cheap, green, efficient and renewable adsorbents to address the issue of heavy metal pollution is highly desired for satisfying the requirements of economy sustainability and water security.
34051463	2	60	theme	heavy	519:523	arg1	metals					525:530	heavy metals	519:530	heavy metals removal	519:538	Herein, a composite aerogel composed of bacterial cellulose (BC) and poly(amidoxime) (PAO) has been fabricated via a facile and scalable self-assembly and in situ oximation transformation for heavy metals removal.
34051463	3	61	theme	high	630:633	arg1	density					635:641	high density	630:641	high density	630:641	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	6	62	theme	PAO/BC	1221:1226	arg1	column					1244:1249	the PAO/BC aerogels-packed column	1217:1249	the PAO/BC aerogels-packed column	1217:1249	Furthermore, the PAO/BC aerogels-packed column can continuously and effectively treat the simulated wastewater with multiple heavy metals coexisting to below the threshold value in the drinking water recommended by World Health Organization (WHO), highlighting its feasibility in the complex environmental water.
34051463	1	63	theme	cheap	136:140	arg1	adsorbents					174:183	Developing cheap, green, efficient and renewable adsorbents	125:183	Developing cheap, green, efficient and renewable adsorbents	125:183	Developing cheap, green, efficient and renewable adsorbents to address the issue of heavy metal pollution is highly desired for satisfying the requirements of economy sustainability and water security.
34051463	4	64	theme	high	1080:1083	arg1	efficiencies					1093:1104	high removal efficiencies	1080:1104	high removal efficiencies	1080:1104	Meanwhile, the sorption equilibrium for the investigated five heavy metals is achieved within 25 min with high removal efficiencies.
34051463	1	65	theme	metal	215:219	arg1	pollution					221:229	heavy metal pollution	209:229	heavy metal pollution	209:229	Developing cheap, green, efficient and renewable adsorbents to address the issue of heavy metal pollution is highly desired for satisfying the requirements of economy sustainability and water security.
34051463	3	66	theme	three-dimensional	568:584	arg1	architecture					613:624	unique three-dimensional (3D) interconnected porous architecture	561:624	unique three-dimensional (3D) interconnected porous architecture	561:624	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	1	67	theme	pollution	221:229	arg1	issue					200:204	the issue	196:204	the issue of heavy metal pollution	196:229	Developing cheap, green, efficient and renewable adsorbents to address the issue of heavy metal pollution is highly desired for satisfying the requirements of economy sustainability and water security.
34051463	2	68	theme	facile	444:449	arg1	self-assembly					464:476	a facile and scalable self-assembly	442:476	a facile and scalable self-assembly	442:476	Herein, a composite aerogel composed of bacterial cellulose (BC) and poly(amidoxime) (PAO) has been fabricated via a facile and scalable self-assembly and in situ oximation transformation for heavy metals removal.
34051463	3	69	theme	reported	948:955	arg1	nano-adsorbents					957:971	most reported nano-adsorbents	943:971	most reported nano-adsorbents	943:971	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
34051463	1	70	theme	green	143:147	arg1	adsorbents					174:183	Developing cheap, green, efficient and renewable adsorbents	125:183	Developing cheap, green, efficient and renewable adsorbents	125:183	Developing cheap, green, efficient and renewable adsorbents to address the issue of heavy metal pollution is highly desired for satisfying the requirements of economy sustainability and water security.
34051463	3	71	theme	sorption	786:793	arg1	capacities					795:804	exceptional sorption capacities	774:804	exceptional sorption capacities	774:804	Benefiting from the unique three-dimensional (3D) interconnected porous architecture and high density of amidoxime functional moieties, the developed PAO/BC composite aerogel is capable of efficiently sequestrating heavy metals with exceptional sorption capacities, e.g. 571.5 mg g-1 for Pb2+, 509.2 mg g-1 for Cu2+, 494 mg g-1 for Zn2+, 457.2 mg g-1 for Mn2+, and 382.3 mg g-1 for Cd2+, outperforming most reported nano-adsorbents.
33688052	0	0	theme	solid-state	87:97	arg1	analysis					108:115	solid-state nanopore analysis	87:115	solid-state nanopore analysis	87:115	Synthetic heparan sulfate standards and machine learning facilitate the development of solid-state nanopore analysis.
33688052	2	1	theme	synthetic	458:466	arg1	chains					488:493	synthetic heparan sulfate GAG chains	458:493	synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route	458:602	Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route.
33688052	1	2	theme	nanopore	154:161	arg1	devices					163:169	solid-state (SS) nanopore devices	137:169	solid-state (SS) nanopore devices	137:169	The application of solid-state (SS) nanopore devices to single-molecule nucleic acid sequencing has been challenging.
33688052	4	3	theme	sample	1016:1021	arg1	zeptomole					1003:1011	zeptomole	1003:1011	zeptomole	1003:1011	By subsequent machine learning, it was possible to determine disaccharide and even monosaccharide composition of these four synthetic GAGs using as few as 500 events, corresponding to a zeptomole of sample.
33688052	2	4	dep	promising	553:561	arg1	developed					573:581	developed	573:581	developed	573:581	Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route.
33688052	5	5	theme	nanopore	1101:1108	arg1	detection					1110:1118	SS nanopore detection	1098:1118	SS nanopore detection	1098:1118	These data suggest that ultrasensitive GAG analysis may be possible using SS nanopore detection and well-characterized molecular training sets.
33688052	2	6	theme	chemoenzymatic	583:596	arg1	route					598:602	a promising, recently developed chemoenzymatic route	551:602	a promising, recently developed chemoenzymatic route	551:602	Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route.
33688052	1	7	theme	devices	163:169	arg1	application					122:132	The application	118:132	The application of solid-state (SS) nanopore devices to single-molecule nucleic acid sequencing	118:212	The application of solid-state (SS) nanopore devices to single-molecule nucleic acid sequencing has been challenging.
33688052	0	8	theme	analysis	108:115	arg1	development					72:82	the development	68:82	the development of solid-state nanopore analysis	68:115	Synthetic heparan sulfate standards and machine learning facilitate the development of solid-state nanopore analysis.
33688052	0	9	theme	nanopore	99:106	arg1	analysis					108:115	solid-state nanopore analysis	87:115	solid-state nanopore analysis	87:115	Synthetic heparan sulfate standards and machine learning facilitate the development of solid-state nanopore analysis.
33688052	3	10	theme	synthetic	801:809	arg1	GAGs					811:814	four synthetic GAGs	796:814	four synthetic GAGs	796:814	A minimal representation of the nanopore data, using only signal magnitude and duration, revealed, by eye and image recognition algorithms, clear differences between the signals generated by four synthetic GAGs.
33688052	4	11	theme	GAGs	951:954	arg1	composition					915:925	disaccharide and even monosaccharide composition	878:925	composition	915:925	By subsequent machine learning, it was possible to determine disaccharide and even monosaccharide composition of these four synthetic GAGs using as few as 500 events, corresponding to a zeptomole of sample.
33688052	4	12	theme	subsequent	820:829	arg1	learning					839:846	subsequent machine learning	820:846	subsequent machine learning	820:846	By subsequent machine learning, it was possible to determine disaccharide and even monosaccharide composition of these four synthetic GAGs using as few as 500 events, corresponding to a zeptomole of sample.
33688052	2	13	theme	nanopore	277:284	arg1	devices					286:292	SS nanopore devices	274:292	SS nanopore devices	274:292	Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route.
33688052	4	14	theme	even	895:898	arg1	composition					915:925	disaccharide and even monosaccharide composition	878:925	composition	915:925	By subsequent machine learning, it was possible to determine disaccharide and even monosaccharide composition of these four synthetic GAGs using as few as 500 events, corresponding to a zeptomole of sample.
33688052	2	15	theme	GAG	484:486	arg1	chains					488:493	synthetic heparan sulfate GAG chains	458:493	synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route	458:602	Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route.
33688052	2	16	theme	SS	274:275	arg1	devices					286:292	SS nanopore devices	274:292	SS nanopore devices	274:292	Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route.
33688052	1	17	theme	single-molecule	174:188	arg1	sequencing					203:212	single-molecule nucleic acid sequencing	174:212	single-molecule nucleic acid sequencing	174:212	The application of solid-state (SS) nanopore devices to single-molecule nucleic acid sequencing has been challenging.
33688052	0	18	theme	heparan	10:16	arg1	standards					26:34	Synthetic heparan sulfate standards	0:34	Synthetic heparan sulfate standards	0:34	Synthetic heparan sulfate standards and machine learning facilitate the development of solid-state nanopore analysis.
33688052	5	19	theme	training	1153:1160	arg1	sets					1162:1165	well-characterized molecular training sets	1124:1165	well-characterized molecular training sets	1124:1165	These data suggest that ultrasensitive GAG analysis may be possible using SS nanopore detection and well-characterized molecular training sets.
33688052	1	20	theme	nucleic	190:196	arg1	sequencing					203:212	single-molecule nucleic acid sequencing	174:212	single-molecule nucleic acid sequencing	174:212	The application of solid-state (SS) nanopore devices to single-molecule nucleic acid sequencing has been challenging.
33688052	0	21	theme	Synthetic	0:8	arg1	standards					26:34	Synthetic heparan sulfate standards	0:34	Synthetic heparan sulfate standards	0:34	Synthetic heparan sulfate standards and machine learning facilitate the development of solid-state nanopore analysis.
33688052	3	22	theme	recognition	721:731	arg1	algorithms					733:742	image recognition algorithms	715:742	image recognition algorithms	715:742	A minimal representation of the nanopore data, using only signal magnitude and duration, revealed, by eye and image recognition algorithms, clear differences between the signals generated by four synthetic GAGs.
33688052	4	23	theme	machine	831:837	arg1	learning					839:846	subsequent machine learning	820:846	subsequent machine learning	820:846	By subsequent machine learning, it was possible to determine disaccharide and even monosaccharide composition of these four synthetic GAGs using as few as 500 events, corresponding to a zeptomole of sample.
33688052	2	24	theme	difficult	306:314	arg1	class					316:320	the more difficult class	297:320	the more difficult class of biopolymer	297:334	Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route.
33688052	1	25	theme	acid	198:201	arg1	sequencing					203:212	single-molecule nucleic acid sequencing	174:212	single-molecule nucleic acid sequencing	174:212	The application of solid-state (SS) nanopore devices to single-molecule nucleic acid sequencing has been challenging.
33688052	2	26	theme	potential	415:423	arg1	use					425:427	the potential use	411:427	the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route	411:602	Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route.
33688052	4	27	theme	monosaccharide	900:913	arg1	composition					915:925	disaccharide and even monosaccharide composition	878:925	composition	915:925	By subsequent machine learning, it was possible to determine disaccharide and even monosaccharide composition of these four synthetic GAGs using as few as 500 events, corresponding to a zeptomole of sample.
33688052	0	28	theme	sulfate	18:24	arg1	standards					26:34	Synthetic heparan sulfate standards	0:34	Synthetic heparan sulfate standards	0:34	Synthetic heparan sulfate standards and machine learning facilitate the development of solid-state nanopore analysis.
33688052	5	29	theme	SS	1098:1099	arg1	detection					1110:1118	SS nanopore detection	1098:1118	SS nanopore detection	1098:1118	These data suggest that ultrasensitive GAG analysis may be possible using SS nanopore detection and well-characterized molecular training sets.
33688052	2	30	theme	sequence	525:532	arg1	chains					488:493	synthetic heparan sulfate GAG chains	458:493	synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route	458:602	Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route.
33688052	3	31	theme	nanopore	637:644	arg1	data					646:649	the nanopore data	633:649	the nanopore data	633:649	A minimal representation of the nanopore data, using only signal magnitude and duration, revealed, by eye and image recognition algorithms, clear differences between the signals generated by four synthetic GAGs.
33688052	0	32	theme	machine	40:46	arg1	learning					48:55	machine learning	40:55	machine learning	40:55	Synthetic heparan sulfate standards and machine learning facilitate the development of solid-state nanopore analysis.
33688052	3	33	theme	image	715:719	arg1	algorithms					733:742	image recognition algorithms	715:742	image recognition algorithms	715:742	A minimal representation of the nanopore data, using only signal magnitude and duration, revealed, by eye and image recognition algorithms, clear differences between the signals generated by four synthetic GAGs.
33688052	3	34	theme	data	646:649	arg1	representation					615:628	A minimal representation	605:628	A minimal representation	605:628	A minimal representation of the nanopore data, using only signal magnitude and duration, revealed, by eye and image recognition algorithms, clear differences between the signals generated by four synthetic GAGs.
33688052	2	35	theme	nanopore	438:445	arg1	use					425:427	the potential use	411:427	the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route	411:602	Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route.
33688052	2	36	theme	composition	509:519	arg1	chains					488:493	synthetic heparan sulfate GAG chains	458:493	synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route	458:602	Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route.
33688052	5	37	theme	ultrasensitive	1048:1061	arg1	analysis					1067:1074	ultrasensitive GAG analysis	1048:1074	ultrasensitive GAG analysis	1048:1074	These data suggest that ultrasensitive GAG analysis may be possible using SS nanopore detection and well-characterized molecular training sets.
33688052	4	38	theme	synthetic	941:949	arg1	GAGs					951:954	these four synthetic GAGs	930:954	these four synthetic GAGs	930:954	By subsequent machine learning, it was possible to determine disaccharide and even monosaccharide composition of these four synthetic GAGs using as few as 500 events, corresponding to a zeptomole of sample.
33688052	2	39	theme	SS	435:436	arg1	nanopore					438:445	an SS nanopore	432:445	an SS nanopore	432:445	Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route.
33688052	2	40	theme	controlled	498:507	arg1	composition					509:519	controlled composition	498:519	controlled composition	498:519	Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route.
33688052	5	41	theme	GAG	1063:1065	arg1	analysis					1067:1074	ultrasensitive GAG analysis	1048:1074	ultrasensitive GAG analysis	1048:1074	These data suggest that ultrasensitive GAG analysis may be possible using SS nanopore detection and well-characterized molecular training sets.
33688052	5	42	theme	molecular	1143:1151	arg1	sets					1162:1165	well-characterized molecular training sets	1124:1165	well-characterized molecular training sets	1124:1165	These data suggest that ultrasensitive GAG analysis may be possible using SS nanopore detection and well-characterized molecular training sets.
33688052	2	43	theme	promising	553:561	arg1	route					598:602	a promising, recently developed chemoenzymatic route	551:602	a promising, recently developed chemoenzymatic route	551:602	Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route.
33688052	3	44	theme	only	658:661	arg1	magnitude					670:678	only signal magnitude	658:678	only signal magnitude	658:678	A minimal representation of the nanopore data, using only signal magnitude and duration, revealed, by eye and image recognition algorithms, clear differences between the signals generated by four synthetic GAGs.
33688052	3	45	theme	clear	745:749	arg1	differences					751:761	clear differences	745:761	clear differences between the signals generated by four synthetic GAGs	745:814	A minimal representation of the nanopore data, using only signal magnitude and duration, revealed, by eye and image recognition algorithms, clear differences between the signals generated by four synthetic GAGs.
33688052	3	46	theme	signal	663:668	arg1	magnitude					670:678	only signal magnitude	658:678	only signal magnitude	658:678	A minimal representation of the nanopore data, using only signal magnitude and duration, revealed, by eye and image recognition algorithms, clear differences between the signals generated by four synthetic GAGs.
33688052	3	47	theme	minimal	607:613	arg1	representation					615:628	A minimal representation	605:628	A minimal representation	605:628	A minimal representation of the nanopore data, using only signal magnitude and duration, revealed, by eye and image recognition algorithms, clear differences between the signals generated by four synthetic GAGs.
33688052	1	48	theme	solid-state	137:147	arg1	devices					163:169	solid-state (SS) nanopore devices	137:169	solid-state (SS) nanopore devices	137:169	The application of solid-state (SS) nanopore devices to single-molecule nucleic acid sequencing has been challenging.
33688052	4	49	theme	disaccharide	878:889	arg1	composition					915:925	disaccharide and even monosaccharide composition	878:925	composition	915:925	By subsequent machine learning, it was possible to determine disaccharide and even monosaccharide composition of these four synthetic GAGs using as few as 500 events, corresponding to a zeptomole of sample.
33688052	2	50	theme	biopolymer	325:334	arg1	class					316:320	the more difficult class	297:320	the more difficult class of biopolymer	297:334	Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route.
33688052	2	51	theme	sulfate	476:482	arg1	chains					488:493	synthetic heparan sulfate GAG chains	458:493	synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route	458:602	Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route.
33688052	1	52	theme	SS	150:151	arg1	devices					163:169	solid-state (SS) nanopore devices	137:169	solid-state (SS) nanopore devices	137:169	The application of solid-state (SS) nanopore devices to single-molecule nucleic acid sequencing has been challenging.
33688052	2	53	theme	heparan	468:474	arg1	chains					488:493	synthetic heparan sulfate GAG chains	458:493	synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route	458:602	Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route.
33688052	2	54	theme	early	246:250	arg1	surprising					374:383	surprising	374:383	surprising	374:383	Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route.
33688052	2	54	theme	early	246:250	arg1	successes					252:260	the early successes	242:260	the early successes in applying SS nanopore devices to the more difficult class of biopolymer	242:334	Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route.
33688052	2	54	theme	early	246:250	arg1	glycosaminoglycans					337:354	glycosaminoglycans	337:354	glycosaminoglycans (GAGs)	337:361	Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route.
33688052	5	55	theme	well-characterized	1124:1141	arg1	sets					1162:1165	well-characterized molecular training sets	1124:1165	well-characterized molecular training sets	1124:1165	These data suggest that ultrasensitive GAG analysis may be possible using SS nanopore detection and well-characterized molecular training sets.
32204018	0	0	theme	bacterial	85:93	arg1	synthesis					115:123	bacterial cellulose templated synthesis	85:123	bacterial cellulose templated synthesis	85:123	Composite scaffolds based on calcium phosphates and barium titanate obtained through bacterial cellulose templated synthesis.
32204018	2	1	theme	resulting	521:529	arg1	system					531:536	the resulting system	517:536	the resulting system	517:536	In this context, calcium phosphates and barium titanate were deposited on bacterial cellulose membranes under ultrasonic irradiation, the resulting system being subjected to a thermal treatment in optimized conditions in order to remove the polymeric template and achieve 3D porous architectures.
32204018	1	2	theme	barium	163:168	arg1	titanate					170:177	barium titanate	163:177	barium titanate	163:177	Taking into account the potential of barium titanate to deliver electrical stimulation to the physiological microenvironment and offer beneficial conditions for the cellular metabolism, this mineral phase was selected for the development of new composites.
32204018	1	3	theme	new	367:369	arg1	composites					371:380	new composites	367:380	new composites	367:380	Taking into account the potential of barium titanate to deliver electrical stimulation to the physiological microenvironment and offer beneficial conditions for the cellular metabolism, this mineral phase was selected for the development of new composites.
32204018	1	4	theme	titanate	170:177	arg1	potential					150:158	the potential	146:158	the potential of barium titanate to deliver electrical stimulation to the physiological microenvironment and offer beneficial conditions for the cellular metabolism	146:309	Taking into account the potential of barium titanate to deliver electrical stimulation to the physiological microenvironment and offer beneficial conditions for the cellular metabolism, this mineral phase was selected for the development of new composites.
32204018	0	5	theme	templated	105:113	arg1	synthesis					115:123	bacterial cellulose templated synthesis	85:123	bacterial cellulose templated synthesis	85:123	Composite scaffolds based on calcium phosphates and barium titanate obtained through bacterial cellulose templated synthesis.
32204018	1	6	theme	composites	371:380	arg1	development					352:362	the development	348:362	the development of new composites	348:380	Taking into account the potential of barium titanate to deliver electrical stimulation to the physiological microenvironment and offer beneficial conditions for the cellular metabolism, this mineral phase was selected for the development of new composites.
32204018	2	7	theme	polymeric	624:632	arg1	template					634:641	the polymeric template	620:641	the polymeric template	620:641	In this context, calcium phosphates and barium titanate were deposited on bacterial cellulose membranes under ultrasonic irradiation, the resulting system being subjected to a thermal treatment in optimized conditions in order to remove the polymeric template and achieve 3D porous architectures.
32204018	1	8	theme	cellular	291:298	arg1	metabolism					300:309	the cellular metabolism	287:309	the cellular metabolism	287:309	Taking into account the potential of barium titanate to deliver electrical stimulation to the physiological microenvironment and offer beneficial conditions for the cellular metabolism, this mineral phase was selected for the development of new composites.
32204018	0	9	theme	cellulose	95:103	arg1	synthesis					115:123	bacterial cellulose templated synthesis	85:123	bacterial cellulose templated synthesis	85:123	Composite scaffolds based on calcium phosphates and barium titanate obtained through bacterial cellulose templated synthesis.
32204018	3	10	theme	such	789:792	arg1	materials					794:802	such materials	789:802	such materials	789:802	The complex characterization performed on the intermediate and final samples demonstrated the suitability of such materials for hard tissue engineering applications.
32204018	2	11	theme	calcium	400:406	arg1	phosphates					408:417	calcium phosphates	400:417	calcium phosphates	400:417	In this context, calcium phosphates and barium titanate were deposited on bacterial cellulose membranes under ultrasonic irradiation, the resulting system being subjected to a thermal treatment in optimized conditions in order to remove the polymeric template and achieve 3D porous architectures.
32204018	2	12	theme	porous	658:663	arg1	architectures					665:677	3D porous architectures	655:677	3D porous architectures	655:677	In this context, calcium phosphates and barium titanate were deposited on bacterial cellulose membranes under ultrasonic irradiation, the resulting system being subjected to a thermal treatment in optimized conditions in order to remove the polymeric template and achieve 3D porous architectures.
32204018	0	13	theme	Composite	0:8	arg1	scaffolds					10:18	Composite scaffolds	0:18	Composite scaffolds based on calcium phosphates and barium	0:57	Composite scaffolds based on calcium phosphates and barium titanate obtained through bacterial cellulose templated synthesis.
32204018	2	14	theme	3D	655:656	arg1	architectures					665:677	3D porous architectures	655:677	3D porous architectures	655:677	In this context, calcium phosphates and barium titanate were deposited on bacterial cellulose membranes under ultrasonic irradiation, the resulting system being subjected to a thermal treatment in optimized conditions in order to remove the polymeric template and achieve 3D porous architectures.
32204018	1	15	theme	electrical	190:199	arg1	stimulation					201:211	electrical stimulation	190:211	electrical stimulation	190:211	Taking into account the potential of barium titanate to deliver electrical stimulation to the physiological microenvironment and offer beneficial conditions for the cellular metabolism, this mineral phase was selected for the development of new composites.
32204018	2	16	theme	cellulose	467:475	arg1	membranes					477:485	bacterial cellulose membranes	457:485	bacterial cellulose membranes	457:485	In this context, calcium phosphates and barium titanate were deposited on bacterial cellulose membranes under ultrasonic irradiation, the resulting system being subjected to a thermal treatment in optimized conditions in order to remove the polymeric template and achieve 3D porous architectures.
32204018	3	17	theme	hard	808:811	arg1	applications					832:843	hard tissue engineering applications	808:843	hard tissue engineering applications	808:843	The complex characterization performed on the intermediate and final samples demonstrated the suitability of such materials for hard tissue engineering applications.
32204018	2	18	theme	bacterial	457:465	arg1	membranes					477:485	bacterial cellulose membranes	457:485	bacterial cellulose membranes	457:485	In this context, calcium phosphates and barium titanate were deposited on bacterial cellulose membranes under ultrasonic irradiation, the resulting system being subjected to a thermal treatment in optimized conditions in order to remove the polymeric template and achieve 3D porous architectures.
32204018	3	19	theme	tissue	813:818	arg1	applications					832:843	hard tissue engineering applications	808:843	hard tissue engineering applications	808:843	The complex characterization performed on the intermediate and final samples demonstrated the suitability of such materials for hard tissue engineering applications.
32204018	1	20	theme	mineral	317:323	arg1	phase					325:329	this mineral phase	312:329	this mineral phase	312:329	Taking into account the potential of barium titanate to deliver electrical stimulation to the physiological microenvironment and offer beneficial conditions for the cellular metabolism, this mineral phase was selected for the development of new composites.
32204018	2	21	theme	optimized	580:588	arg1	conditions					590:599	optimized conditions	580:599	optimized conditions	580:599	In this context, calcium phosphates and barium titanate were deposited on bacterial cellulose membranes under ultrasonic irradiation, the resulting system being subjected to a thermal treatment in optimized conditions in order to remove the polymeric template and achieve 3D porous architectures.
32204018	3	22	theme	complex	684:690	arg1	characterization					692:707	The complex characterization	680:707	The complex characterization performed on the intermediate and final samples	680:755	The complex characterization performed on the intermediate and final samples demonstrated the suitability of such materials for hard tissue engineering applications.
32204018	0	23	dep	titanate	59:66	arg1	obtained					68:75	obtained	68:75	titanate obtained through bacterial cellulose templated synthesis	59:123	Composite scaffolds based on calcium phosphates and barium titanate obtained through bacterial cellulose templated synthesis.
32204018	1	24	theme	physiological	220:232	arg1	microenvironment					234:249	the physiological microenvironment	216:249	the physiological microenvironment	216:249	Taking into account the potential of barium titanate to deliver electrical stimulation to the physiological microenvironment and offer beneficial conditions for the cellular metabolism, this mineral phase was selected for the development of new composites.
32204018	0	25	theme	calcium	29:35	arg1	phosphates					37:46	calcium phosphates	29:46	calcium phosphates	29:46	Composite scaffolds based on calcium phosphates and barium titanate obtained through bacterial cellulose templated synthesis.
32204018	2	26	theme	barium	423:428	arg1	titanate					430:437	barium titanate	423:437	barium titanate	423:437	In this context, calcium phosphates and barium titanate were deposited on bacterial cellulose membranes under ultrasonic irradiation, the resulting system being subjected to a thermal treatment in optimized conditions in order to remove the polymeric template and achieve 3D porous architectures.
32204018	2	27	theme	ultrasonic	493:502	arg1	irradiation					504:514	ultrasonic irradiation	493:514	ultrasonic irradiation	493:514	In this context, calcium phosphates and barium titanate were deposited on bacterial cellulose membranes under ultrasonic irradiation, the resulting system being subjected to a thermal treatment in optimized conditions in order to remove the polymeric template and achieve 3D porous architectures.
32204018	2	28	theme	thermal	559:565	arg1	treatment					567:575	a thermal treatment	557:575	a thermal treatment in optimized conditions	557:599	In this context, calcium phosphates and barium titanate were deposited on bacterial cellulose membranes under ultrasonic irradiation, the resulting system being subjected to a thermal treatment in optimized conditions in order to remove the polymeric template and achieve 3D porous architectures.
32204018	3	29	theme	final	743:747	arg1	samples					749:755	the intermediate and final samples	722:755	the intermediate and final samples	722:755	The complex characterization performed on the intermediate and final samples demonstrated the suitability of such materials for hard tissue engineering applications.
32204018	3	30	theme	engineering	820:830	arg1	applications					832:843	hard tissue engineering applications	808:843	hard tissue engineering applications	808:843	The complex characterization performed on the intermediate and final samples demonstrated the suitability of such materials for hard tissue engineering applications.
32204018	3	31	theme	intermediate	726:737	arg1	samples					749:755	the intermediate and final samples	722:755	the intermediate and final samples	722:755	The complex characterization performed on the intermediate and final samples demonstrated the suitability of such materials for hard tissue engineering applications.
32204018	3	32	theme	materials	794:802	arg1	suitability					774:784	the suitability	770:784	the suitability of such materials for hard tissue engineering applications	770:843	The complex characterization performed on the intermediate and final samples demonstrated the suitability of such materials for hard tissue engineering applications.
32204018	2	33	from	treatment	567:575	arg1	conditions					590:599	optimized conditions	580:599	optimized conditions	580:599	In this context, calcium phosphates and barium titanate were deposited on bacterial cellulose membranes under ultrasonic irradiation, the resulting system being subjected to a thermal treatment in optimized conditions in order to remove the polymeric template and achieve 3D porous architectures.
32204018	1	34	theme	beneficial	261:270	arg1	conditions					272:281	beneficial conditions	261:281	beneficial conditions for the cellular metabolism	261:309	Taking into account the potential of barium titanate to deliver electrical stimulation to the physiological microenvironment and offer beneficial conditions for the cellular metabolism, this mineral phase was selected for the development of new composites.
32750480	3	0	from	wastewater	571:580	arg1	removal					547:553	effective removal	537:553	effective removal of Cd ions from wastewater	537:580	Chitosan biopolymer encloses a great deal of amino and hydroxyl groups, which provide effective removal of Cd ions from wastewater.
32750480	6	1	theme	R2	815:816	arg1	values					818:823	the R2 values	811:823	the R2 values	811:823	Based on the R2 values, it was evidenced that the adsorption process was best fitted with the Freundlich isotherm than Langmuir.
32750480	9	2	theme	ions	1304:1307	arg1	removal					1290:1296	the removal	1286:1296	the removal of Cd ions	1286:1307	The overall evaluation proved that though both the adsorbents are suitable for the removal of Cd ions, the efficiency of CS-based ternary composite material is better than PP-based composite.
32750480	0	3	theme	fiber	73:77	arg1	composite					79:87	fiber composite	73:87	fiber composite	73:87	Toxic heavy metal cadmium removal using chitosan and polypropylene based fiber composite.
32750480	3	4	theme	ions	561:564	arg1	removal					547:553	effective removal	537:553	effective removal of Cd ions from wastewater	537:580	Chitosan biopolymer encloses a great deal of amino and hydroxyl groups, which provide effective removal of Cd ions from wastewater.
32750480	1	5	theme	water	328:332	arg1	waste					334:338	water waste	328:338	water waste	328:338	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	1	6	theme	performance	138:148	arg1	BF					203:204	BF	203:204	BF	203:204	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	1	6	theme	performance	138:148	arg1	fiber					196:200	the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber	134:200	the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF)	134:205	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	2	7	theme	various	429:435	arg1	applications					437:448	various applications	429:448	various applications	429:448	Polypropylene is harnessed for its importance in forming strong composite materials for various applications.
32750480	1	8	theme	composite	255:263	arg1	materials					265:273	composite materials	255:273	composite materials	255:273	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	8	9	from	wastewater	1195:1204	arg1	removal					1172:1178	the removal	1168:1178	the removal of Cd ion from wastewater	1168:1204	Kinetics studies revealed that the pseudo-second-order model was followed during the removal of Cd ion from wastewater.
32750480	3	10	theme	Chitosan	451:458	arg1	biopolymer					460:469	Chitosan biopolymer	451:469	Chitosan biopolymer	451:469	Chitosan biopolymer encloses a great deal of amino and hydroxyl groups, which provide effective removal of Cd ions from wastewater.
32750480	4	11	theme	ions	642:645	arg1	pH-dependent					651:662	pH-dependent	651:662	pH-dependent	651:662	The batch adsorption studies proved that the removal of Cd ions was pH-dependent and attained optimum at pH 5.5 for both the composites.
32750480	4	11	theme	ions	642:645	arg1	removal					628:634	the removal	624:634	the removal of Cd ions	624:645	The batch adsorption studies proved that the removal of Cd ions was pH-dependent and attained optimum at pH 5.5 for both the composites.
32750480	4	12	theme	Cd	639:640	arg1	ions					642:645	Cd ions	639:645	Cd ions	639:645	The batch adsorption studies proved that the removal of Cd ions was pH-dependent and attained optimum at pH 5.5 for both the composites.
32750480	8	13	theme	ion	1186:1188	arg1	removal					1172:1178	the removal	1168:1178	the removal of Cd ion from wastewater	1168:1204	Kinetics studies revealed that the pseudo-second-order model was followed during the removal of Cd ion from wastewater.
32750480	4	14	theme	adsorption	593:602	arg1	studies					604:610	The batch adsorption studies	583:610	The batch adsorption studies	583:610	The batch adsorption studies proved that the removal of Cd ions was pH-dependent and attained optimum at pH 5.5 for both the composites.
32750480	9	15	theme	overall	1211:1217	arg1	evaluation					1219:1228	The overall evaluation	1207:1228	The overall evaluation	1207:1228	The overall evaluation proved that though both the adsorbents are suitable for the removal of Cd ions, the efficiency of CS-based ternary composite material is better than PP-based composite.
32750480	5	16	theme	Freundlich	733:742	arg1	models					744:749	Langmuir and Freundlich models	720:749	Langmuir and Freundlich models	720:749	Langmuir and Freundlich models were applied for the obtained experimental values.
32750480	8	17	theme	Kinetics	1087:1094	arg1	studies					1096:1102	Kinetics studies	1087:1102	Kinetics studies	1087:1102	Kinetics studies revealed that the pseudo-second-order model was followed during the removal of Cd ion from wastewater.
32750480	1	18	theme	of	150:151	arg1	BF					203:204	BF	203:204	BF	203:204	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	1	18	theme	of	150:151	arg1	fiber					196:200	the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber	134:200	the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF)	134:205	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	2	19	theme	composite	405:413	arg1	materials					415:423	strong composite materials	398:423	strong composite materials for various applications	398:448	Polypropylene is harnessed for its importance in forming strong composite materials for various applications.
32750480	7	20	theme	hybrid	965:970	arg1	Cmax = 419 mg/g					983:997	Cmax = 419 mg/g	983:997	Cmax = 419 mg/g	983:997	The sorption capacity of CS/SF/BF hybrid composite (Cmax = 419 mg/g) is higher than PP/SF/BF composite (Cmax = 304 mg/g), and allows multilayer adsorption.
32750480	7	20	theme	hybrid	965:970	arg1	composite					972:980	CS/SF/BF hybrid composite	956:980	CS/SF/BF hybrid composite (Cmax = 419 mg/g)	956:998	The sorption capacity of CS/SF/BF hybrid composite (Cmax = 419 mg/g) is higher than PP/SF/BF composite (Cmax = 304 mg/g), and allows multilayer adsorption.
32750480	7	21	theme	multilayer	1064:1073	arg1	adsorption					1075:1084	multilayer adsorption	1064:1084	multilayer adsorption	1064:1084	The sorption capacity of CS/SF/BF hybrid composite (Cmax = 419 mg/g) is higher than PP/SF/BF composite (Cmax = 304 mg/g), and allows multilayer adsorption.
32750480	1	22	theme	polypropylene	153:165	arg1	BF					203:204	BF	203:204	BF	203:204	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	1	22	theme	polypropylene	153:165	arg1	fiber					196:200	the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber	134:200	the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF)	134:205	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	0	23	theme	heavy	6:10	arg1	cadmium					18:24	heavy metal cadmium	6:24	Toxic heavy metal cadmium removal using chitosan and polypropylene	0:65	Toxic heavy metal cadmium removal using chitosan and polypropylene based fiber composite.
32750480	1	24	theme	adsorptive	283:292	arg1	removal					294:300	the adsorptive removal	279:300	the adsorptive removal of cadmium (Cd) ions from water waste	279:338	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	7	25	theme	composite	972:980	arg1	higher					1003:1008	higher	1003:1008	higher	1003:1008	The sorption capacity of CS/SF/BF hybrid composite (Cmax = 419 mg/g) is higher than PP/SF/BF composite (Cmax = 304 mg/g), and allows multilayer adsorption.
32750480	7	25	theme	composite	972:980	arg1	capacity					944:951	The sorption capacity	931:951	The sorption capacity of CS/SF/BF hybrid composite (Cmax = 419 mg/g)	931:998	The sorption capacity of CS/SF/BF hybrid composite (Cmax = 419 mg/g) is higher than PP/SF/BF composite (Cmax = 304 mg/g), and allows multilayer adsorption.
32750480	3	26	theme	great	482:486	arg1	deal					488:491	a great deal	480:491	a great deal	480:491	Chitosan biopolymer encloses a great deal of amino and hydroxyl groups, which provide effective removal of Cd ions from wastewater.
32750480	9	27	theme	ternary	1337:1343	arg1	material					1355:1362	CS-based ternary composite material	1328:1362	CS-based ternary composite material	1328:1362	The overall evaluation proved that though both the adsorbents are suitable for the removal of Cd ions, the efficiency of CS-based ternary composite material is better than PP-based composite.
32750480	0	28	theme	Toxic	0:4	arg1	removal					26:32	Toxic heavy metal cadmium removal	0:32	Toxic heavy metal cadmium removal using chitosan and polypropylene	0:65	Toxic heavy metal cadmium removal using chitosan and polypropylene based fiber composite.
32750480	1	29	theme	chitosan-based	211:224	arg1	hybrid					226:231	chitosan-based hybrid	211:231	chitosan-based hybrid (chitosan(CS)/SF)/BF)	211:253	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	1	29	theme	chitosan-based	211:224	arg1	/BF					250:252	chitosan(CS)/SF)/BF	234:252	chitosan(CS)/SF)/BF	234:252	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	1	30	theme	PP	168:169	arg1	BF					203:204	BF	203:204	BF	203:204	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	1	30	theme	PP	168:169	arg1	fiber					196:200	the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber	134:200	the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF)	134:205	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	0	31	theme	cadmium	18:24	arg1	removal					26:32	Toxic heavy metal cadmium removal	0:32	Toxic heavy metal cadmium removal using chitosan and polypropylene	0:65	Toxic heavy metal cadmium removal using chitosan and polypropylene based fiber composite.
32750480	6	32	theme	adsorption	852:861	arg1	process					863:869	the adsorption process	848:869	the adsorption process	848:869	Based on the R2 values, it was evidenced that the adsorption process was best fitted with the Freundlich isotherm than Langmuir.
32750480	7	33	theme	CS/SF/BF	956:963	arg1	Cmax = 419 mg/g					983:997	Cmax = 419 mg/g	983:997	Cmax = 419 mg/g	983:997	The sorption capacity of CS/SF/BF hybrid composite (Cmax = 419 mg/g) is higher than PP/SF/BF composite (Cmax = 304 mg/g), and allows multilayer adsorption.
32750480	7	33	theme	CS/SF/BF	956:963	arg1	composite					972:980	CS/SF/BF hybrid composite	956:980	CS/SF/BF hybrid composite (Cmax = 419 mg/g)	956:998	The sorption capacity of CS/SF/BF hybrid composite (Cmax = 419 mg/g) is higher than PP/SF/BF composite (Cmax = 304 mg/g), and allows multilayer adsorption.
32750480	3	34	theme	Cd	558:559	arg1	ions					561:564	Cd ions	558:564	Cd ions	558:564	Chitosan biopolymer encloses a great deal of amino and hydroxyl groups, which provide effective removal of Cd ions from wastewater.
32750480	9	35	theme	material	1355:1362	arg1	composite					1388:1396	better than PP-based composite	1367:1396	better than PP-based composite	1367:1396	The overall evaluation proved that though both the adsorbents are suitable for the removal of Cd ions, the efficiency of CS-based ternary composite material is better than PP-based composite.
32750480	9	35	theme	material	1355:1362	arg1	efficiency					1314:1323	the efficiency	1310:1323	the efficiency of CS-based ternary composite material	1310:1362	The overall evaluation proved that though both the adsorbents are suitable for the removal of Cd ions, the efficiency of CS-based ternary composite material is better than PP-based composite.
32750480	0	36	theme	metal	12:16	arg1	cadmium					18:24	heavy metal cadmium	6:24	Toxic heavy metal cadmium removal using chitosan and polypropylene	0:65	Toxic heavy metal cadmium removal using chitosan and polypropylene based fiber composite.
32750480	6	37	theme	Freundlich	896:905	arg1	isotherm					907:914	the Freundlich isotherm	892:914	the Freundlich isotherm than Langmuir	892:928	Based on the R2 values, it was evidenced that the adsorption process was best fitted with the Freundlich isotherm than Langmuir.
32750480	1	38	from	waste	334:338	arg1	removal					294:300	the adsorptive removal	279:300	the adsorptive removal of cadmium (Cd) ions from water waste	279:338	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	3	39	theme	effective	537:545	arg1	removal					547:553	effective removal	537:553	effective removal of Cd ions from wastewater	537:580	Chitosan biopolymer encloses a great deal of amino and hydroxyl groups, which provide effective removal of Cd ions from wastewater.
32750480	1	40	theme	/sisal	171:176	arg1	BF					203:204	BF	203:204	BF	203:204	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	1	40	theme	/sisal	171:176	arg1	fiber					196:200	the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber	134:200	the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF)	134:205	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	1	41	theme	chitosan	234:241	arg1	CS					243:244	CS	243:244	CS	243:244	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	1	41	theme	chitosan	234:241	arg1	/BF					250:252	chitosan(CS)/SF)/BF	234:252	chitosan(CS)/SF)/BF	234:252	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	1	41	theme	chitosan	234:241	arg1	hybrid					226:231	chitosan-based hybrid	211:231	chitosan-based hybrid (chitosan(CS)/SF)/BF)	211:253	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	1	42	dep	fiber	196:200	arg1	materials					265:273	composite materials	255:273	composite materials	255:273	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	5	43	theme	obtained	772:779	arg1	values					794:799	the obtained experimental values	768:799	the obtained experimental values	768:799	Langmuir and Freundlich models were applied for the obtained experimental values.
32750480	1	44	theme	fiber	178:182	arg1	BF					203:204	BF	203:204	BF	203:204	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	1	44	theme	fiber	178:182	arg1	fiber					196:200	the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber	134:200	the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF)	134:205	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	1	45	theme	cadmium	305:311	arg1	ions					318:321	cadmium (Cd) ions	305:321	cadmium (Cd) ions	305:321	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	3	46	theme	hydroxyl	506:513	arg1	groups					515:520	amino and hydroxyl groups	496:520	groups	515:520	Chitosan biopolymer encloses a great deal of amino and hydroxyl groups, which provide effective removal of Cd ions from wastewater.
32750480	9	47	theme	than	1374:1377	arg1	composite					1388:1396	better than PP-based composite	1367:1396	better than PP-based composite	1367:1396	The overall evaluation proved that though both the adsorbents are suitable for the removal of Cd ions, the efficiency of CS-based ternary composite material is better than PP-based composite.
32750480	9	47	theme	than	1374:1377	arg1	efficiency					1314:1323	the efficiency	1310:1323	the efficiency of CS-based ternary composite material	1310:1362	The overall evaluation proved that though both the adsorbents are suitable for the removal of Cd ions, the efficiency of CS-based ternary composite material is better than PP-based composite.
32750480	7	48	theme	PP/SF/BF	1015:1022	arg1	Cmax = 304 mg/g					1035:1049	Cmax = 304 mg/g	1035:1049	Cmax = 304 mg/g	1035:1049	The sorption capacity of CS/SF/BF hybrid composite (Cmax = 419 mg/g) is higher than PP/SF/BF composite (Cmax = 304 mg/g), and allows multilayer adsorption.
32750480	7	48	theme	PP/SF/BF	1015:1022	arg1	composite					1024:1032	PP/SF/BF composite	1015:1032	PP/SF/BF composite (Cmax = 304 mg/g)	1015:1050	The sorption capacity of CS/SF/BF hybrid composite (Cmax = 419 mg/g) is higher than PP/SF/BF composite (Cmax = 304 mg/g), and allows multilayer adsorption.
32750480	5	49	theme	experimental	781:792	arg1	values					794:799	the obtained experimental values	768:799	the obtained experimental values	768:799	Langmuir and Freundlich models were applied for the obtained experimental values.
32750480	9	50	theme	better	1367:1372	arg1	composite					1388:1396	better than PP-based composite	1367:1396	better than PP-based composite	1367:1396	The overall evaluation proved that though both the adsorbents are suitable for the removal of Cd ions, the efficiency of CS-based ternary composite material is better than PP-based composite.
32750480	9	50	theme	better	1367:1372	arg1	efficiency					1314:1323	the efficiency	1310:1323	the efficiency of CS-based ternary composite material	1310:1362	The overall evaluation proved that though both the adsorbents are suitable for the removal of Cd ions, the efficiency of CS-based ternary composite material is better than PP-based composite.
32750480	9	51	theme	PP-based	1379:1386	arg1	composite					1388:1396	better than PP-based composite	1367:1396	better than PP-based composite	1367:1396	The overall evaluation proved that though both the adsorbents are suitable for the removal of Cd ions, the efficiency of CS-based ternary composite material is better than PP-based composite.
32750480	9	51	theme	PP-based	1379:1386	arg1	efficiency					1314:1323	the efficiency	1310:1323	the efficiency of CS-based ternary composite material	1310:1362	The overall evaluation proved that though both the adsorbents are suitable for the removal of Cd ions, the efficiency of CS-based ternary composite material is better than PP-based composite.
32750480	7	52	theme	sorption	935:942	arg1	higher					1003:1008	higher	1003:1008	higher	1003:1008	The sorption capacity of CS/SF/BF hybrid composite (Cmax = 419 mg/g) is higher than PP/SF/BF composite (Cmax = 304 mg/g), and allows multilayer adsorption.
32750480	7	52	theme	sorption	935:942	arg1	capacity					944:951	The sorption capacity	931:951	The sorption capacity of CS/SF/BF hybrid composite (Cmax = 419 mg/g)	931:998	The sorption capacity of CS/SF/BF hybrid composite (Cmax = 419 mg/g) is higher than PP/SF/BF composite (Cmax = 304 mg/g), and allows multilayer adsorption.
32750480	1	53	theme	Cd	314:315	arg1	ions					318:321	cadmium (Cd) ions	305:321	cadmium (Cd) ions	305:321	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	3	54	theme	amino	496:500	arg1	groups					515:520	amino and hydroxyl groups	496:520	groups	515:520	Chitosan biopolymer encloses a great deal of amino and hydroxyl groups, which provide effective removal of Cd ions from wastewater.
32750480	1	55	theme	present	105:111	arg1	work					113:116	the present work	101:116	the present work	101:116	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	1	56	theme	/SF	246:248	arg1	CS					243:244	CS	243:244	CS	243:244	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	1	56	theme	/SF	246:248	arg1	/BF					250:252	chitosan(CS)/SF)/BF	234:252	chitosan(CS)/SF)/BF	234:252	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	1	56	theme	/SF	246:248	arg1	hybrid					226:231	chitosan-based hybrid	211:231	chitosan-based hybrid (chitosan(CS)/SF)/BF)	211:253	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	8	57	theme	Cd	1183:1184	arg1	ion					1186:1188	Cd ion	1183:1188	Cd ion	1183:1188	Kinetics studies revealed that the pseudo-second-order model was followed during the removal of Cd ion from wastewater.
32750480	1	58	theme	work	113:116	arg1	aim					94:96	The aim	90:96	The aim of the present work	90:116	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	9	59	theme	composite	1345:1353	arg1	material					1355:1362	CS-based ternary composite material	1328:1362	CS-based ternary composite material	1328:1362	The overall evaluation proved that though both the adsorbents are suitable for the removal of Cd ions, the efficiency of CS-based ternary composite material is better than PP-based composite.
32750480	1	60	theme	SF	185:186	arg1	BF					203:204	BF	203:204	BF	203:204	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	1	60	theme	SF	185:186	arg1	fiber					196:200	the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber	134:200	the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF)	134:205	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	1	61	theme	ions	318:321	arg1	removal					294:300	the adsorptive removal	279:300	the adsorptive removal of cadmium (Cd) ions from water waste	279:338	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	2	62	theme	strong	398:403	arg1	materials					415:423	strong composite materials	398:423	strong composite materials for various applications	398:448	Polypropylene is harnessed for its importance in forming strong composite materials for various applications.
32750480	3	63	theme	groups	515:520	arg1	deal					488:491	a great deal	480:491	a great deal	480:491	Chitosan biopolymer encloses a great deal of amino and hydroxyl groups, which provide effective removal of Cd ions from wastewater.
32750480	4	64	theme	batch	587:591	arg1	studies					604:610	The batch adsorption studies	583:610	The batch adsorption studies	583:610	The batch adsorption studies proved that the removal of Cd ions was pH-dependent and attained optimum at pH 5.5 for both the composites.
32750480	9	65	theme	CS-based	1328:1335	arg1	material					1355:1362	CS-based ternary composite material	1328:1362	CS-based ternary composite material	1328:1362	The overall evaluation proved that though both the adsorbents are suitable for the removal of Cd ions, the efficiency of CS-based ternary composite material is better than PP-based composite.
32750480	1	66	theme	/banana	188:194	arg1	BF					203:204	BF	203:204	BF	203:204	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	1	66	theme	/banana	188:194	arg1	fiber					196:200	the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber	134:200	the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF)	134:205	The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste.
32750480	8	67	theme	pseudo-second-order	1122:1140	arg1	model					1142:1146	the pseudo-second-order model	1118:1146	the pseudo-second-order model	1118:1146	Kinetics studies revealed that the pseudo-second-order model was followed during the removal of Cd ion from wastewater.
32750480	5	68	theme	Langmuir	720:727	arg1	models					744:749	Langmuir and Freundlich models	720:749	Langmuir and Freundlich models	720:749	Langmuir and Freundlich models were applied for the obtained experimental values.
32750480	9	69	theme	Cd	1301:1302	arg1	ions					1304:1307	Cd ions	1301:1307	Cd ions	1301:1307	The overall evaluation proved that though both the adsorbents are suitable for the removal of Cd ions, the efficiency of CS-based ternary composite material is better than PP-based composite.
34111483	5	0	theme	starch	669:674	arg1	incorporation					639:651	the incorporation	635:651	the incorporation of the oxidized starch into starch/polyvinyl alcohol/glyceryl composite films	635:729	With the incorporation of the oxidized starch into starch/polyvinyl alcohol/glyceryl composite films, the mechanical property and transparency of the films improved substantially.
34111483	9	1	theme	composite	1390:1398	arg1	film					1400:1403	this starch-based composite film	1372:1403	this starch-based composite film	1372:1403	In conclusion, cellulose nanocrystal acts as prooxidant and reforcing agent in this starch-based composite film, which makes them promising materials in the preparation of novel composite materials.
34111483	4	2	theme	oxidized	596:603	arg1	starch					605:610	oxidized starch	596:610	oxidized starch	596:610	The function of cellulose nanocrystal on the physicochemical properties of oxidized starch was investigated.
34111483	0	3	theme	starch	75:80	arg1	oxidation					51:59	oxidation	51:59	oxidation of starch and starch	51:80	Physicochemical impact of cellulose nanocrystal on oxidation of starch and starch based composite films.
34111483	0	4	from	impact	16:21	arg1	oxidation					51:59	oxidation	51:59	oxidation of starch and starch	51:80	Physicochemical impact of cellulose nanocrystal on oxidation of starch and starch based composite films.
34111483	6	5	theme	0.5 wt	864:869	arg1	dosage					894:899	0.5 wt% cellulose nanocrystal dosage	864:899	0.5 wt% cellulose nanocrystal dosage	864:899	The highest oxidation level of starch was attained at 0.5 wt% cellulose nanocrystal dosage and the carboxylate content was 1.10%, the composite film showed the maximum transparency of 0.66 and highest contact angle of 102.0°.
34111483	4	6	theme	nanocrystal	547:557	arg1	function					525:532	The function	521:532	The function of cellulose nanocrystal on the physicochemical properties of oxidized starch	521:610	The function of cellulose nanocrystal on the physicochemical properties of oxidized starch was investigated.
34111483	1	7	theme	green	244:248	arg1	method					250:255	an alternative and green method	225:255	an alternative and green method to improve the oxidizing degree of oxidized starch	225:306	A cellulose nanocrystal based oxidation system is developed for oxidizing starch in the presence of NaClO, and provides an alternative and green method to improve the oxidizing degree of oxidized starch.
34111483	8	8	theme	cellulose	1194:1202	arg1	starch					1231:1236	the cellulose nanocrystal-based oxidized starch	1190:1236	the cellulose nanocrystal-based oxidized starch	1190:1236	Compared to native starch and TEMPO-oxidated starch, the cellulose nanocrystal-based oxidized starch improved the hydrophobicity of film more substanially.
34111483	5	9	theme	starch/polyvinyl	681:696	arg1	films					725:729	starch/polyvinyl alcohol/glyceryl composite films	681:729	starch/polyvinyl alcohol/glyceryl composite films	681:729	With the incorporation of the oxidized starch into starch/polyvinyl alcohol/glyceryl composite films, the mechanical property and transparency of the films improved substantially.
34111483	0	10	theme	composite	88:96	arg1	films					98:102	composite films	88:102	composite films	88:102	Physicochemical impact of cellulose nanocrystal on oxidation of starch and starch based composite films.
34111483	5	11	theme	alcohol/glyceryl	698:713	arg1	films					725:729	starch/polyvinyl alcohol/glyceryl composite films	681:729	starch/polyvinyl alcohol/glyceryl composite films	681:729	With the incorporation of the oxidized starch into starch/polyvinyl alcohol/glyceryl composite films, the mechanical property and transparency of the films improved substantially.
34111483	6	12	theme	maximum	970:976	arg1	transparency					978:989	the maximum transparency	966:989	the maximum transparency of 0.66	966:997	The highest oxidation level of starch was attained at 0.5 wt% cellulose nanocrystal dosage and the carboxylate content was 1.10%, the composite film showed the maximum transparency of 0.66 and highest contact angle of 102.0°.
34111483	9	13	theme	promising	1423:1431	arg1	materials					1433:1441	promising materials	1423:1441	promising materials	1423:1441	In conclusion, cellulose nanocrystal acts as prooxidant and reforcing agent in this starch-based composite film, which makes them promising materials in the preparation of novel composite materials.
34111483	4	14	theme	starch	605:610	arg1	properties					582:591	the physicochemical properties	562:591	the physicochemical properties of oxidized starch	562:610	The function of cellulose nanocrystal on the physicochemical properties of oxidized starch was investigated.
34111483	5	15	theme	films	780:784	arg1	property					747:754	property	747:754	property	747:754	With the incorporation of the oxidized starch into starch/polyvinyl alcohol/glyceryl composite films, the mechanical property and transparency of the films improved substantially.
34111483	5	15	theme	films	780:784	arg1	transparency					760:771	transparency	760:771	transparency	760:771	With the incorporation of the oxidized starch into starch/polyvinyl alcohol/glyceryl composite films, the mechanical property and transparency of the films improved substantially.
34111483	8	16	theme	oxidized	1222:1229	arg1	starch					1231:1236	the cellulose nanocrystal-based oxidized starch	1190:1236	the cellulose nanocrystal-based oxidized starch	1190:1236	Compared to native starch and TEMPO-oxidated starch, the cellulose nanocrystal-based oxidized starch improved the hydrophobicity of film more substanially.
34111483	9	17	theme	starch-based	1377:1388	arg1	film					1400:1403	this starch-based composite film	1372:1403	this starch-based composite film	1372:1403	In conclusion, cellulose nanocrystal acts as prooxidant and reforcing agent in this starch-based composite film, which makes them promising materials in the preparation of novel composite materials.
34111483	2	18	theme	scanning	384:391	arg1	microscopy					393:402	confocal laser scanning microscopy	369:402	confocal laser scanning microscopy	369:402	The underlying mechanism for the oxidation was studied with confocal laser scanning microscopy.
34111483	8	19	theme	nanocrystal-based	1204:1220	arg1	starch					1231:1236	the cellulose nanocrystal-based oxidized starch	1190:1236	the cellulose nanocrystal-based oxidized starch	1190:1236	Compared to native starch and TEMPO-oxidated starch, the cellulose nanocrystal-based oxidized starch improved the hydrophobicity of film more substanially.
34111483	9	20	theme	materials	1481:1489	arg1	preparation					1450:1460	the preparation	1446:1460	the preparation of novel composite materials	1446:1489	In conclusion, cellulose nanocrystal acts as prooxidant and reforcing agent in this starch-based composite film, which makes them promising materials in the preparation of novel composite materials.
34111483	9	21	theme	composite	1471:1479	arg1	materials					1481:1489	novel composite materials	1465:1489	novel composite materials	1465:1489	In conclusion, cellulose nanocrystal acts as prooxidant and reforcing agent in this starch-based composite film, which makes them promising materials in the preparation of novel composite materials.
34111483	6	22	theme	starch	841:846	arg1	level					832:836	The highest oxidation level	810:836	The highest oxidation level of starch	810:846	The highest oxidation level of starch was attained at 0.5 wt% cellulose nanocrystal dosage and the carboxylate content was 1.10%, the composite film showed the maximum transparency of 0.66 and highest contact angle of 102.0°.
34111483	2	23	theme	laser	378:382	arg1	microscopy					393:402	confocal laser scanning microscopy	369:402	confocal laser scanning microscopy	369:402	The underlying mechanism for the oxidation was studied with confocal laser scanning microscopy.
34111483	5	24	dep	property	747:754	arg1	the					732:734	the	732:734	the	732:734	With the incorporation of the oxidized starch into starch/polyvinyl alcohol/glyceryl composite films, the mechanical property and transparency of the films improved substantially.
34111483	7	25	theme	%	1112:1112	arg1	nanocrystal					1124:1134	1.0 wt% cellulose nanocrystal	1106:1134	1.0 wt% cellulose nanocrystal	1106:1134	Meanwhile, the best film was obtained with oxidized starch containing 1.0 wt% cellulose nanocrystal.
34111483	2	26	theme	confocal	369:376	arg1	microscopy					393:402	confocal laser scanning microscopy	369:402	confocal laser scanning microscopy	369:402	The underlying mechanism for the oxidation was studied with confocal laser scanning microscopy.
34111483	0	27	theme	Physicochemical	0:14	arg1	impact					16:21	Physicochemical impact	0:21	Physicochemical impact of cellulose nanocrystal on oxidation of starch and starch	0:80	Physicochemical impact of cellulose nanocrystal on oxidation of starch and starch based composite films.
34111483	6	28	theme	contact	1011:1017	arg1	angle					1019:1023	highest contact angle	1003:1023	highest contact angle of 102.0°	1003:1033	The highest oxidation level of starch was attained at 0.5 wt% cellulose nanocrystal dosage and the carboxylate content was 1.10%, the composite film showed the maximum transparency of 0.66 and highest contact angle of 102.0°.
34111483	5	29	theme	oxidized	660:667	arg1	starch					669:674	the oxidized starch	656:674	the oxidized starch	656:674	With the incorporation of the oxidized starch into starch/polyvinyl alcohol/glyceryl composite films, the mechanical property and transparency of the films improved substantially.
34111483	9	30	theme	cellulose	1308:1316	arg1	nanocrystal					1318:1328	cellulose nanocrystal	1308:1328	cellulose nanocrystal	1308:1328	In conclusion, cellulose nanocrystal acts as prooxidant and reforcing agent in this starch-based composite film, which makes them promising materials in the preparation of novel composite materials.
34111483	0	31	theme	cellulose	26:34	arg1	nanocrystal					36:46	cellulose nanocrystal	26:46	cellulose nanocrystal	26:46	Physicochemical impact of cellulose nanocrystal on oxidation of starch and starch based composite films.
34111483	6	32	theme	highest	1003:1009	arg1	angle					1019:1023	highest contact angle	1003:1023	highest contact angle of 102.0°	1003:1033	The highest oxidation level of starch was attained at 0.5 wt% cellulose nanocrystal dosage and the carboxylate content was 1.10%, the composite film showed the maximum transparency of 0.66 and highest contact angle of 102.0°.
34111483	5	33	theme	mechanical	736:745	arg1	property					747:754	property	747:754	property	747:754	With the incorporation of the oxidized starch into starch/polyvinyl alcohol/glyceryl composite films, the mechanical property and transparency of the films improved substantially.
34111483	6	34	theme	oxidation	822:830	arg1	level					832:836	The highest oxidation level	810:836	The highest oxidation level of starch	810:846	The highest oxidation level of starch was attained at 0.5 wt% cellulose nanocrystal dosage and the carboxylate content was 1.10%, the composite film showed the maximum transparency of 0.66 and highest contact angle of 102.0°.
34111483	1	35	theme	oxidizing	272:280	arg1	degree					282:287	the oxidizing degree	268:287	the oxidizing degree of oxidized starch	268:306	A cellulose nanocrystal based oxidation system is developed for oxidizing starch in the presence of NaClO, and provides an alternative and green method to improve the oxidizing degree of oxidized starch.
34111483	7	36	theme	1.0 wt	1106:1111	arg1	nanocrystal					1124:1134	1.0 wt% cellulose nanocrystal	1106:1134	1.0 wt% cellulose nanocrystal	1106:1134	Meanwhile, the best film was obtained with oxidized starch containing 1.0 wt% cellulose nanocrystal.
34111483	7	37	theme	cellulose	1114:1122	arg1	nanocrystal					1124:1134	1.0 wt% cellulose nanocrystal	1106:1134	1.0 wt% cellulose nanocrystal	1106:1134	Meanwhile, the best film was obtained with oxidized starch containing 1.0 wt% cellulose nanocrystal.
34111483	8	38	theme	film	1269:1272	arg1	hydrophobicity					1251:1264	the hydrophobicity	1247:1264	the hydrophobicity of film	1247:1272	Compared to native starch and TEMPO-oxidated starch, the cellulose nanocrystal-based oxidized starch improved the hydrophobicity of film more substanially.
34111483	4	39	theme	physicochemical	566:580	arg1	properties					582:591	the physicochemical properties	562:591	the physicochemical properties of oxidized starch	562:610	The function of cellulose nanocrystal on the physicochemical properties of oxidized starch was investigated.
34111483	7	40	theme	oxidized	1079:1086	arg1	starch					1088:1093	oxidized starch	1079:1093	oxidized starch containing 1.0 wt% cellulose nanocrystal	1079:1134	Meanwhile, the best film was obtained with oxidized starch containing 1.0 wt% cellulose nanocrystal.
34111483	6	41	theme	carboxylate	909:919	arg1	%					937:937	1.10%	933:937	1.10%	933:937	The highest oxidation level of starch was attained at 0.5 wt% cellulose nanocrystal dosage and the carboxylate content was 1.10%, the composite film showed the maximum transparency of 0.66 and highest contact angle of 102.0°.
34111483	6	41	theme	carboxylate	909:919	arg1	content					921:927	the carboxylate content	905:927	the carboxylate content	905:927	The highest oxidation level of starch was attained at 0.5 wt% cellulose nanocrystal dosage and the carboxylate content was 1.10%, the composite film showed the maximum transparency of 0.66 and highest contact angle of 102.0°.
34111483	6	42	theme	0.66	994:997	arg1	transparency					978:989	the maximum transparency	966:989	the maximum transparency of 0.66	966:997	The highest oxidation level of starch was attained at 0.5 wt% cellulose nanocrystal dosage and the carboxylate content was 1.10%, the composite film showed the maximum transparency of 0.66 and highest contact angle of 102.0°.
34111483	6	42	theme	0.66	994:997	arg1	angle					1019:1023	highest contact angle	1003:1023	highest contact angle of 102.0°	1003:1033	The highest oxidation level of starch was attained at 0.5 wt% cellulose nanocrystal dosage and the carboxylate content was 1.10%, the composite film showed the maximum transparency of 0.66 and highest contact angle of 102.0°.
34111483	7	43	contain	containing	1095:1104	arg1	starch					1088:1093	oxidized starch	1079:1093	oxidized starch containing 1.0 wt% cellulose nanocrystal	1079:1134	Meanwhile, the best film was obtained with oxidized starch containing 1.0 wt% cellulose nanocrystal.
34111483	7	43	contain	containing	1095:1104	arg2	nanocrystal					1124:1134	1.0 wt% cellulose nanocrystal	1106:1134	1.0 wt% cellulose nanocrystal	1106:1134	Meanwhile, the best film was obtained with oxidized starch containing 1.0 wt% cellulose nanocrystal.
34111483	1	44	theme	NaClO	205:209	arg1	presence					193:200	the presence	189:200	the presence of NaClO	189:209	A cellulose nanocrystal based oxidation system is developed for oxidizing starch in the presence of NaClO, and provides an alternative and green method to improve the oxidizing degree of oxidized starch.
34111483	0	45	theme	nanocrystal	36:46	arg1	impact					16:21	Physicochemical impact	0:21	Physicochemical impact of cellulose nanocrystal on oxidation of starch and starch	0:80	Physicochemical impact of cellulose nanocrystal on oxidation of starch and starch based composite films.
34111483	6	46	theme	102.0°	1028:1033	arg1	transparency					978:989	the maximum transparency	966:989	the maximum transparency of 0.66	966:997	The highest oxidation level of starch was attained at 0.5 wt% cellulose nanocrystal dosage and the carboxylate content was 1.10%, the composite film showed the maximum transparency of 0.66 and highest contact angle of 102.0°.
34111483	6	46	theme	102.0°	1028:1033	arg1	angle					1019:1023	highest contact angle	1003:1023	highest contact angle of 102.0°	1003:1033	The highest oxidation level of starch was attained at 0.5 wt% cellulose nanocrystal dosage and the carboxylate content was 1.10%, the composite film showed the maximum transparency of 0.66 and highest contact angle of 102.0°.
34111483	6	47	theme	highest	814:820	arg1	level					832:836	The highest oxidation level	810:836	The highest oxidation level of starch	810:846	The highest oxidation level of starch was attained at 0.5 wt% cellulose nanocrystal dosage and the carboxylate content was 1.10%, the composite film showed the maximum transparency of 0.66 and highest contact angle of 102.0°.
34111483	1	48	theme	oxidized	292:299	arg1	starch					301:306	oxidized starch	292:306	oxidized starch	292:306	A cellulose nanocrystal based oxidation system is developed for oxidizing starch in the presence of NaClO, and provides an alternative and green method to improve the oxidizing degree of oxidized starch.
34111483	5	49	theme	composite	715:723	arg1	films					725:729	starch/polyvinyl alcohol/glyceryl composite films	681:729	starch/polyvinyl alcohol/glyceryl composite films	681:729	With the incorporation of the oxidized starch into starch/polyvinyl alcohol/glyceryl composite films, the mechanical property and transparency of the films improved substantially.
34111483	3	50	theme	cellulose	423:431	arg1	nanocrystal					433:443	cellulose nanocrystal	423:443	cellulose nanocrystal	423:443	It was found that cellulose nanocrystal would penetrate into the starch microparticles and contribute to oxidation.
34111483	7	51	theme	best	1051:1054	arg1	film					1056:1059	the best film	1047:1059	the best film	1047:1059	Meanwhile, the best film was obtained with oxidized starch containing 1.0 wt% cellulose nanocrystal.
34111483	7	51	theme	best	1051:1054	arg1	Meanwhile					1036:1044	Meanwhile	1036:1044	Meanwhile	1036:1044	Meanwhile, the best film was obtained with oxidized starch containing 1.0 wt% cellulose nanocrystal.
34111483	1	52	theme	cellulose	107:115	arg1	system					145:150	A cellulose nanocrystal based oxidation system	105:150	A cellulose nanocrystal based oxidation system	105:150	A cellulose nanocrystal based oxidation system is developed for oxidizing starch in the presence of NaClO, and provides an alternative and green method to improve the oxidizing degree of oxidized starch.
34111483	4	53	from	function	525:532	arg1	properties					582:591	the physicochemical properties	562:591	the physicochemical properties of oxidized starch	562:610	The function of cellulose nanocrystal on the physicochemical properties of oxidized starch was investigated.
34111483	1	54	theme	starch	301:306	arg1	degree					282:287	the oxidizing degree	268:287	the oxidizing degree of oxidized starch	268:306	A cellulose nanocrystal based oxidation system is developed for oxidizing starch in the presence of NaClO, and provides an alternative and green method to improve the oxidizing degree of oxidized starch.
34111483	3	55	theme	starch	470:475	arg1	microparticles					477:490	the starch microparticles	466:490	the starch microparticles	466:490	It was found that cellulose nanocrystal would penetrate into the starch microparticles and contribute to oxidation.
34111483	6	56	theme	composite	944:952	arg1	film					954:957	the composite film	940:957	the composite film	940:957	The highest oxidation level of starch was attained at 0.5 wt% cellulose nanocrystal dosage and the carboxylate content was 1.10%, the composite film showed the maximum transparency of 0.66 and highest contact angle of 102.0°.
34111483	1	57	theme	nanocrystal	117:127	arg1	system					145:150	A cellulose nanocrystal based oxidation system	105:150	A cellulose nanocrystal based oxidation system	105:150	A cellulose nanocrystal based oxidation system is developed for oxidizing starch in the presence of NaClO, and provides an alternative and green method to improve the oxidizing degree of oxidized starch.
34111483	4	58	theme	cellulose	537:545	arg1	nanocrystal					547:557	cellulose nanocrystal	537:557	cellulose nanocrystal	537:557	The function of cellulose nanocrystal on the physicochemical properties of oxidized starch was investigated.
34111483	6	59	theme	nanocrystal	882:892	arg1	dosage					894:899	0.5 wt% cellulose nanocrystal dosage	864:899	0.5 wt% cellulose nanocrystal dosage	864:899	The highest oxidation level of starch was attained at 0.5 wt% cellulose nanocrystal dosage and the carboxylate content was 1.10%, the composite film showed the maximum transparency of 0.66 and highest contact angle of 102.0°.
34111483	1	60	theme	based	129:133	arg1	system					145:150	A cellulose nanocrystal based oxidation system	105:150	A cellulose nanocrystal based oxidation system	105:150	A cellulose nanocrystal based oxidation system is developed for oxidizing starch in the presence of NaClO, and provides an alternative and green method to improve the oxidizing degree of oxidized starch.
34111483	8	61	theme	native	1149:1154	arg1	starch					1156:1161	native starch	1149:1161	native starch	1149:1161	Compared to native starch and TEMPO-oxidated starch, the cellulose nanocrystal-based oxidized starch improved the hydrophobicity of film more substanially.
34111483	6	62	theme	cellulose	872:880	arg1	dosage					894:899	0.5 wt% cellulose nanocrystal dosage	864:899	0.5 wt% cellulose nanocrystal dosage	864:899	The highest oxidation level of starch was attained at 0.5 wt% cellulose nanocrystal dosage and the carboxylate content was 1.10%, the composite film showed the maximum transparency of 0.66 and highest contact angle of 102.0°.
34111483	1	63	theme	oxidation	135:143	arg1	system					145:150	A cellulose nanocrystal based oxidation system	105:150	A cellulose nanocrystal based oxidation system	105:150	A cellulose nanocrystal based oxidation system is developed for oxidizing starch in the presence of NaClO, and provides an alternative and green method to improve the oxidizing degree of oxidized starch.
34111483	2	64	theme	underlying	313:322	arg1	mechanism					324:332	The underlying mechanism	309:332	The underlying mechanism for the oxidation	309:350	The underlying mechanism for the oxidation was studied with confocal laser scanning microscopy.
34111483	6	65	theme	%	870:870	arg1	dosage					894:899	0.5 wt% cellulose nanocrystal dosage	864:899	0.5 wt% cellulose nanocrystal dosage	864:899	The highest oxidation level of starch was attained at 0.5 wt% cellulose nanocrystal dosage and the carboxylate content was 1.10%, the composite film showed the maximum transparency of 0.66 and highest contact angle of 102.0°.
34111483	9	66	theme	reforcing	1353:1361	arg1	agent					1363:1367	prooxidant and reforcing agent	1338:1367	prooxidant and reforcing agent	1338:1367	In conclusion, cellulose nanocrystal acts as prooxidant and reforcing agent in this starch-based composite film, which makes them promising materials in the preparation of novel composite materials.
34111483	0	67	theme	starch	64:69	arg1	oxidation					51:59	oxidation	51:59	oxidation of starch and starch	51:80	Physicochemical impact of cellulose nanocrystal on oxidation of starch and starch based composite films.
34111483	9	68	theme	prooxidant	1338:1347	arg1	agent					1363:1367	prooxidant and reforcing agent	1338:1367	prooxidant and reforcing agent	1338:1367	In conclusion, cellulose nanocrystal acts as prooxidant and reforcing agent in this starch-based composite film, which makes them promising materials in the preparation of novel composite materials.
34111483	9	69	theme	novel	1465:1469	arg1	materials					1481:1489	novel composite materials	1465:1489	novel composite materials	1465:1489	In conclusion, cellulose nanocrystal acts as prooxidant and reforcing agent in this starch-based composite film, which makes them promising materials in the preparation of novel composite materials.
34111483	1	70	theme	alternative	228:238	arg1	method					250:255	an alternative and green method	225:255	an alternative and green method to improve the oxidizing degree of oxidized starch	225:306	A cellulose nanocrystal based oxidation system is developed for oxidizing starch in the presence of NaClO, and provides an alternative and green method to improve the oxidizing degree of oxidized starch.
34428490	0	0	theme	dodecanoic	68:77	arg1	acid					79:82	dodecanoic acid	68:82	dodecanoic acid influenced by the mixed-solvent composition	68:126	The rate and evenness of the substitutions on hyaluronan grafted by dodecanoic acid influenced by the mixed-solvent composition.
34428490	4	1	theme	enzymatic	826:834	arg1	degradation					836:846	enzymatic degradation	826:846	enzymatic degradation	826:846	The chemical characterization of the prepared derivatives was elucidated by NMR, FTIR spectroscopy, thermal analyses, and gas chromatography, while the distribution of substituents was evaluated by enzymatic degradation.
34428490	3	2	from	distribution	475:486	arg1	water					532:536	water	532:536	water	532:536	The effect of the cosolvent was studied and showed an even distribution of substituents and higher reaction rate in water: 1,4-dioxane compared to water:tert-butanol where substituents occupy adjacent positions.
34428490	0	3	from	rate	4:7	arg1	hyaluronan					46:55	hyaluronan	46:55	hyaluronan	46:55	The rate and evenness of the substitutions on hyaluronan grafted by dodecanoic acid influenced by the mixed-solvent composition.
34428490	1	4	theme	low	143:145	arg1	17 kDa					165:170	17 kDa	165:170	17 kDa	165:170	In this work, low molecular weight (17 kDa) hyaluronan was modified by dodecanoyl substituents.
34428490	1	4	theme	low	143:145	arg1	weight					157:162	low molecular weight	143:162	low molecular weight (17 kDa) hyaluronan	143:182	In this work, low molecular weight (17 kDa) hyaluronan was modified by dodecanoyl substituents.
34428490	2	5	theme	anhydride	327:335	arg1	preparation					304:314	the preparation	300:314	the preparation of a mixed anhydride, which reacts in a second step with HA in water mixed with an organic solvent	300:413	The activation of dodecanoic acid was mediated by benzoyl chloride towards the preparation of a mixed anhydride, which reacts in a second step with HA in water mixed with an organic solvent.
34428490	6	6	theme	bound	1156:1160	arg1	substituents					1162:1173	the already bound substituents	1144:1173	the already bound substituents	1144:1173	The resulting incompatibility of solvation-shells of the two entities repels the reaction intermediates from the HA chain and drives them towards the already bound substituents, explaining the observed differences in the distribution evenness.
34428490	3	7	theme	higher	508:513	arg1	rate					524:527	higher reaction rate	508:527	higher reaction rate	508:527	The effect of the cosolvent was studied and showed an even distribution of substituents and higher reaction rate in water: 1,4-dioxane compared to water:tert-butanol where substituents occupy adjacent positions.
34428490	3	8	theme	substituents	491:502	arg1	distribution					475:486	an even distribution	467:486	an even distribution of substituents and higher reaction rate in water: 1,4-dioxane compared to water:tert-butanol where substituents occupy adjacent positions	467:625	The effect of the cosolvent was studied and showed an even distribution of substituents and higher reaction rate in water: 1,4-dioxane compared to water:tert-butanol where substituents occupy adjacent positions.
34428490	6	9	theme	HA	1111:1112	arg1	chain					1114:1118	the HA chain	1107:1118	the HA chain	1107:1118	The resulting incompatibility of solvation-shells of the two entities repels the reaction intermediates from the HA chain and drives them towards the already bound substituents, explaining the observed differences in the distribution evenness.
34428490	0	10	from	substitutions	29:41	arg1	hyaluronan					46:55	hyaluronan	46:55	hyaluronan	46:55	The rate and evenness of the substitutions on hyaluronan grafted by dodecanoic acid influenced by the mixed-solvent composition.
34428490	4	11	theme	substituents	796:807	arg1	distribution					780:791	the distribution	776:791	the distribution of substituents	776:807	The chemical characterization of the prepared derivatives was elucidated by NMR, FTIR spectroscopy, thermal analyses, and gas chromatography, while the distribution of substituents was evaluated by enzymatic degradation.
34428490	7	12	from	influence	1252:1260	arg1	selectivity					1293:1303	the reaction selectivity	1280:1303	the reaction selectivity	1280:1303	Thus, the influence of the solvent on the reaction selectivity is observed by shielding reactive sites around HA.
34428490	3	13	theme	even	470:473	arg1	distribution					475:486	an even distribution	467:486	an even distribution of substituents and higher reaction rate in water: 1,4-dioxane compared to water:tert-butanol where substituents occupy adjacent positions	467:625	The effect of the cosolvent was studied and showed an even distribution of substituents and higher reaction rate in water: 1,4-dioxane compared to water:tert-butanol where substituents occupy adjacent positions.
34428490	7	14	theme	solvent	1269:1275	arg1	influence					1252:1260	the influence	1248:1260	the influence of the solvent on the reaction selectivity	1248:1303	Thus, the influence of the solvent on the reaction selectivity is observed by shielding reactive sites around HA.
34428490	1	15	theme	molecular	147:155	arg1	17 kDa					165:170	17 kDa	165:170	17 kDa	165:170	In this work, low molecular weight (17 kDa) hyaluronan was modified by dodecanoyl substituents.
34428490	1	15	theme	molecular	147:155	arg1	weight					157:162	low molecular weight	143:162	low molecular weight (17 kDa) hyaluronan	143:182	In this work, low molecular weight (17 kDa) hyaluronan was modified by dodecanoyl substituents.
34428490	5	16	theme	opposite	887:894	arg1	separations					904:914	opposite solvent separations	887:914	opposite solvent separations	887:914	Molecular-dynamics simulations reveal opposite solvent separations around HA and dodecanoyl chains, that is stronger in water:tert-butanol solution.
34428490	2	17	theme	acid	254:257	arg1	activation					229:238	The activation	225:238	The activation of dodecanoic acid	225:257	The activation of dodecanoic acid was mediated by benzoyl chloride towards the preparation of a mixed anhydride, which reacts in a second step with HA in water mixed with an organic solvent.
34428490	3	18	theme	cosolvent	434:442	arg1	effect					420:425	The effect	416:425	The effect of the cosolvent	416:442	The effect of the cosolvent was studied and showed an even distribution of substituents and higher reaction rate in water: 1,4-dioxane compared to water:tert-butanol where substituents occupy adjacent positions.
34428490	1	19	theme	weight	157:162	arg1	hyaluronan					173:182	low molecular weight (17 kDa) hyaluronan	143:182	low molecular weight (17 kDa) hyaluronan	143:182	In this work, low molecular weight (17 kDa) hyaluronan was modified by dodecanoyl substituents.
34428490	2	20	theme	organic	399:405	arg1	solvent					407:413	an organic solvent	396:413	an organic solvent	396:413	The activation of dodecanoic acid was mediated by benzoyl chloride towards the preparation of a mixed anhydride, which reacts in a second step with HA in water mixed with an organic solvent.
34428490	3	21	theme	reaction	515:522	arg1	rate					524:527	higher reaction rate	508:527	higher reaction rate	508:527	The effect of the cosolvent was studied and showed an even distribution of substituents and higher reaction rate in water: 1,4-dioxane compared to water:tert-butanol where substituents occupy adjacent positions.
34428490	6	22	theme	reaction	1079:1086	arg1	intermediates					1088:1100	the reaction intermediates	1075:1100	the reaction intermediates from the HA chain	1075:1118	The resulting incompatibility of solvation-shells of the two entities repels the reaction intermediates from the HA chain and drives them towards the already bound substituents, explaining the observed differences in the distribution evenness.
34428490	6	23	theme	entities	1059:1066	arg1	solvation-shells					1031:1046	solvation-shells	1031:1046	solvation-shells of the two entities	1031:1066	The resulting incompatibility of solvation-shells of the two entities repels the reaction intermediates from the HA chain and drives them towards the already bound substituents, explaining the observed differences in the distribution evenness.
34428490	2	24	with	step	363:366	arg1	HA					373:374	HA	373:374	HA	373:374	The activation of dodecanoic acid was mediated by benzoyl chloride towards the preparation of a mixed anhydride, which reacts in a second step with HA in water mixed with an organic solvent.
34428490	6	25	theme	observed	1191:1198	arg1	differences					1200:1210	the observed differences	1187:1210	the observed differences in the distribution evenness	1187:1239	The resulting incompatibility of solvation-shells of the two entities repels the reaction intermediates from the HA chain and drives them towards the already bound substituents, explaining the observed differences in the distribution evenness.
34428490	3	26	theme	rate	524:527	arg1	distribution					475:486	an even distribution	467:486	an even distribution of substituents and higher reaction rate in water: 1,4-dioxane compared to water:tert-butanol where substituents occupy adjacent positions	467:625	The effect of the cosolvent was studied and showed an even distribution of substituents and higher reaction rate in water: 1,4-dioxane compared to water:tert-butanol where substituents occupy adjacent positions.
34428490	5	27	theme	tert-butanol	975:986	arg1	solution					988:995	water:tert-butanol solution	969:995	water:tert-butanol solution	969:995	Molecular-dynamics simulations reveal opposite solvent separations around HA and dodecanoyl chains, that is stronger in water:tert-butanol solution.
34428490	5	28	theme	solvent	896:902	arg1	separations					904:914	opposite solvent separations	887:914	opposite solvent separations	887:914	Molecular-dynamics simulations reveal opposite solvent separations around HA and dodecanoyl chains, that is stronger in water:tert-butanol solution.
34428490	4	29	theme	FTIR	709:712	arg1	spectroscopy					714:725	FTIR spectroscopy	709:725	FTIR spectroscopy	709:725	The chemical characterization of the prepared derivatives was elucidated by NMR, FTIR spectroscopy, thermal analyses, and gas chromatography, while the distribution of substituents was evaluated by enzymatic degradation.
34428490	5	30	theme	dodecanoyl	930:939	arg1	chains					941:946	HA and dodecanoyl chains	923:946	HA and dodecanoyl chains	923:946	Molecular-dynamics simulations reveal opposite solvent separations around HA and dodecanoyl chains, that is stronger in water:tert-butanol solution.
34428490	0	31	theme	mixed-solvent	102:114	arg1	composition					116:126	the mixed-solvent composition	98:126	the mixed-solvent composition	98:126	The rate and evenness of the substitutions on hyaluronan grafted by dodecanoic acid influenced by the mixed-solvent composition.
34428490	2	32	theme	benzoyl	275:281	arg1	chloride					283:290	benzoyl chloride	275:290	benzoyl chloride towards the preparation of a mixed anhydride, which reacts in a second step with HA in water mixed with an organic solvent	275:413	The activation of dodecanoic acid was mediated by benzoyl chloride towards the preparation of a mixed anhydride, which reacts in a second step with HA in water mixed with an organic solvent.
34428490	5	33	theme	Molecular-dynamics	849:866	arg1	simulations					868:878	Molecular-dynamics simulations	849:878	Molecular-dynamics simulations	849:878	Molecular-dynamics simulations reveal opposite solvent separations around HA and dodecanoyl chains, that is stronger in water:tert-butanol solution.
34428490	4	34	theme	prepared	665:672	arg1	derivatives					674:684	the prepared derivatives	661:684	the prepared derivatives	661:684	The chemical characterization of the prepared derivatives was elucidated by NMR, FTIR spectroscopy, thermal analyses, and gas chromatography, while the distribution of substituents was evaluated by enzymatic degradation.
34428490	6	35	theme	solvation-shells	1031:1046	arg1	incompatibility					1012:1026	The resulting incompatibility	998:1026	The resulting incompatibility of solvation-shells of the two entities	998:1066	The resulting incompatibility of solvation-shells of the two entities repels the reaction intermediates from the HA chain and drives them towards the already bound substituents, explaining the observed differences in the distribution evenness.
34428490	6	36	from	chain	1114:1118	arg1	intermediates					1088:1100	the reaction intermediates	1075:1100	the reaction intermediates from the HA chain	1075:1118	The resulting incompatibility of solvation-shells of the two entities repels the reaction intermediates from the HA chain and drives them towards the already bound substituents, explaining the observed differences in the distribution evenness.
34428490	7	37	theme	reaction	1284:1291	arg1	selectivity					1293:1303	the reaction selectivity	1280:1303	the reaction selectivity	1280:1303	Thus, the influence of the solvent on the reaction selectivity is observed by shielding reactive sites around HA.
34428490	8	38	used	used	1470:1473	arg2	control					1369:1375	a control	1367:1375	a control of the distribution of the substituents	1367:1415	Therefore, a control of the distribution of the substituents was obtained by defining the concentration of HA and used cosolvent.
34428490	0	39	from	hyaluronan	46:55	arg1	evenness					13:20	evenness	13:20	evenness	13:20	The rate and evenness of the substitutions on hyaluronan grafted by dodecanoic acid influenced by the mixed-solvent composition.
34428490	0	39	from	hyaluronan	46:55	arg1	rate					4:7	rate	4:7	rate	4:7	The rate and evenness of the substitutions on hyaluronan grafted by dodecanoic acid influenced by the mixed-solvent composition.
34428490	6	40	theme	distribution	1219:1230	arg1	evenness					1232:1239	the distribution evenness	1215:1239	the distribution evenness	1215:1239	The resulting incompatibility of solvation-shells of the two entities repels the reaction intermediates from the HA chain and drives them towards the already bound substituents, explaining the observed differences in the distribution evenness.
34428490	2	41	theme	mixed	321:325	arg1	anhydride					327:335	a mixed anhydride	319:335	a mixed anhydride	319:335	The activation of dodecanoic acid was mediated by benzoyl chloride towards the preparation of a mixed anhydride, which reacts in a second step with HA in water mixed with an organic solvent.
34428490	0	42	theme	substitutions	29:41	arg1	evenness					13:20	evenness	13:20	evenness	13:20	The rate and evenness of the substitutions on hyaluronan grafted by dodecanoic acid influenced by the mixed-solvent composition.
34428490	0	42	theme	substitutions	29:41	arg1	rate					4:7	rate	4:7	rate	4:7	The rate and evenness of the substitutions on hyaluronan grafted by dodecanoic acid influenced by the mixed-solvent composition.
34428490	8	43	theme	HA	1463:1464	arg1	concentration					1446:1458	the concentration	1442:1458	the concentration of HA	1442:1464	Therefore, a control of the distribution of the substituents was obtained by defining the concentration of HA and used cosolvent.
34428490	8	44	theme	distribution	1384:1395	arg1	control					1369:1375	a control	1367:1375	a control of the distribution of the substituents	1367:1415	Therefore, a control of the distribution of the substituents was obtained by defining the concentration of HA and used cosolvent.
34428490	2	45	from	step	363:366	arg1	water					379:383	water	379:383	water mixed with an organic solvent	379:413	The activation of dodecanoic acid was mediated by benzoyl chloride towards the preparation of a mixed anhydride, which reacts in a second step with HA in water mixed with an organic solvent.
34428490	3	46	dep	distribution	475:486	arg1	tert-butanol					569:580	tert-butanol	569:580	an even distribution of substituents and higher reaction rate in water: 1,4-dioxane compared to water:tert-butanol where substituents occupy adjacent positions	467:625	The effect of the cosolvent was studied and showed an even distribution of substituents and higher reaction rate in water: 1,4-dioxane compared to water:tert-butanol where substituents occupy adjacent positions.
34428490	3	46	dep	distribution	475:486	arg1	1,4-dioxane					539:549	1,4-dioxane	539:549	an even distribution of substituents and higher reaction rate in water: 1,4-dioxane compared to water:tert-butanol where substituents occupy adjacent positions	467:625	The effect of the cosolvent was studied and showed an even distribution of substituents and higher reaction rate in water: 1,4-dioxane compared to water:tert-butanol where substituents occupy adjacent positions.
34428490	8	47	theme	substituents	1404:1415	arg1	distribution					1384:1395	the distribution	1380:1395	the distribution of the substituents	1380:1415	Therefore, a control of the distribution of the substituents was obtained by defining the concentration of HA and used cosolvent.
34428490	4	48	theme	derivatives	674:684	arg1	characterization					641:656	The chemical characterization	628:656	The chemical characterization of the prepared derivatives	628:684	The chemical characterization of the prepared derivatives was elucidated by NMR, FTIR spectroscopy, thermal analyses, and gas chromatography, while the distribution of substituents was evaluated by enzymatic degradation.
34428490	5	49	theme	water	969:973	arg1	solution					988:995	water:tert-butanol solution	969:995	water:tert-butanol solution	969:995	Molecular-dynamics simulations reveal opposite solvent separations around HA and dodecanoyl chains, that is stronger in water:tert-butanol solution.
34428490	2	50	theme	dodecanoic	243:252	arg1	acid					254:257	dodecanoic acid	243:257	dodecanoic acid	243:257	The activation of dodecanoic acid was mediated by benzoyl chloride towards the preparation of a mixed anhydride, which reacts in a second step with HA in water mixed with an organic solvent.
34428490	6	51	theme	resulting	1002:1010	arg1	incompatibility					1012:1026	The resulting incompatibility	998:1026	The resulting incompatibility of solvation-shells of the two entities	998:1066	The resulting incompatibility of solvation-shells of the two entities repels the reaction intermediates from the HA chain and drives them towards the already bound substituents, explaining the observed differences in the distribution evenness.
34428490	4	52	theme	thermal	728:734	arg1	analyses					736:743	thermal analyses	728:743	thermal analyses	728:743	The chemical characterization of the prepared derivatives was elucidated by NMR, FTIR spectroscopy, thermal analyses, and gas chromatography, while the distribution of substituents was evaluated by enzymatic degradation.
34428490	3	53	theme	adjacent	608:615	arg1	positions					617:625	adjacent positions	608:625	adjacent positions	608:625	The effect of the cosolvent was studied and showed an even distribution of substituents and higher reaction rate in water: 1,4-dioxane compared to water:tert-butanol where substituents occupy adjacent positions.
34428490	5	54	theme	HA	923:924	arg1	chains					941:946	HA and dodecanoyl chains	923:946	HA and dodecanoyl chains	923:946	Molecular-dynamics simulations reveal opposite solvent separations around HA and dodecanoyl chains, that is stronger in water:tert-butanol solution.
34428490	7	55	theme	reactive	1330:1337	arg1	sites					1339:1343	reactive sites	1330:1343	reactive sites around HA	1330:1353	Thus, the influence of the solvent on the reaction selectivity is observed by shielding reactive sites around HA.
34428490	1	56	theme	dodecanoyl	200:209	arg1	substituents					211:222	dodecanoyl substituents	200:222	dodecanoyl substituents	200:222	In this work, low molecular weight (17 kDa) hyaluronan was modified by dodecanoyl substituents.
34428490	6	57	from	differences	1200:1210	arg1	evenness					1232:1239	the distribution evenness	1215:1239	the distribution evenness	1215:1239	The resulting incompatibility of solvation-shells of the two entities repels the reaction intermediates from the HA chain and drives them towards the already bound substituents, explaining the observed differences in the distribution evenness.
34428490	0	58	dep	rate	4:7	arg1	The					0:2	The	0:2	The	0:2	The rate and evenness of the substitutions on hyaluronan grafted by dodecanoic acid influenced by the mixed-solvent composition.
34428490	1	59	mod	modified	188:195	arg1	hyaluronan					173:182	low molecular weight (17 kDa) hyaluronan	143:182	low molecular weight (17 kDa) hyaluronan	143:182	In this work, low molecular weight (17 kDa) hyaluronan was modified by dodecanoyl substituents.
34428490	1	59	mod	modified	188:195	arg3	substituents					211:222	dodecanoyl substituents	200:222	dodecanoyl substituents	200:222	In this work, low molecular weight (17 kDa) hyaluronan was modified by dodecanoyl substituents.
34428490	2	60	theme	second	356:361	arg1	step					363:366	a second step	354:366	a second step with HA in water mixed with an organic solvent	354:413	The activation of dodecanoic acid was mediated by benzoyl chloride towards the preparation of a mixed anhydride, which reacts in a second step with HA in water mixed with an organic solvent.
34428490	0	61	from	evenness	13:20	arg1	hyaluronan					46:55	hyaluronan	46:55	hyaluronan	46:55	The rate and evenness of the substitutions on hyaluronan grafted by dodecanoic acid influenced by the mixed-solvent composition.
34428490	4	62	theme	gas	750:752	arg1	chromatography					754:767	gas chromatography	750:767	gas chromatography	750:767	The chemical characterization of the prepared derivatives was elucidated by NMR, FTIR spectroscopy, thermal analyses, and gas chromatography, while the distribution of substituents was evaluated by enzymatic degradation.
34428490	4	63	theme	chemical	632:639	arg1	characterization					641:656	The chemical characterization	628:656	The chemical characterization of the prepared derivatives	628:684	The chemical characterization of the prepared derivatives was elucidated by NMR, FTIR spectroscopy, thermal analyses, and gas chromatography, while the distribution of substituents was evaluated by enzymatic degradation.
33234593	9	0	theme	separation	1409:1418	arg1	conditions					1420:1429	the separation conditions	1405:1429	the separation conditions	1405:1429	Using reverse-phase ion-pair HPLC with a postcolumn fluorescent labeling system, the separation conditions were optimized to quantify the 13 units.
33234593	4	1	theme	due	694:696	arg1	part					689:692	part	689:692	part due to the lack of a standard method to analyze this rare modification	689:763	However, the physiological roles of 3-O-sulfates produced by other 3OST isoforms, which do not bind AT, remain obscure, in part due to the lack of a standard method to analyze this rare modification.
33234593	12	2	theme	first	1732:1736	arg1	This					1720:1723	This	1720:1723	This	1720:1723	This is the first report that shows the quantification of three non-AT-binding 3-O-sulfated units and establishes a new approach to explore the physiological functions of 3-O-sulfate.
33234593	12	2	theme	first	1732:1736	arg1	report					1738:1743	the first report	1728:1743	the first report that shows the quantification of three non-AT-binding 3-O-sulfated units and establishes a new approach to explore the physiological functions of 3-O-sulfate	1728:1901	This is the first report that shows the quantification of three non-AT-binding 3-O-sulfated units and establishes a new approach to explore the physiological functions of 3-O-sulfate.
33234593	4	3	theme	3-O-sulfates	602:613	arg1	roles					593:597	the physiological roles	575:597	the physiological roles	575:597	However, the physiological roles of 3-O-sulfates produced by other 3OST isoforms, which do not bind AT, remain obscure, in part due to the lack of a standard method to analyze this rare modification.
33234593	1	4	theme	numerous	167:174	arg1	proteins					176:183	numerous proteins	167:183	numerous proteins	167:183	Heparan sulfate is synthesized by most animal cells and interacts with numerous proteins via specific sulfation motifs to regulate various physiological processes.
33234593	6	5	theme	3-O-sulfated	986:997	arg1	products					1106:1113	digestion products	1096:1113	digestion products of heparin lyases	1096:1131	We previously examined the reaction products of human 3OST isoforms and identified five 3-O-sulfated components, including three non-AT-binding disaccharides and two AT-binding tetrasaccharides, as digestion products of heparin lyases.
33234593	6	5	theme	3-O-sulfated	986:997	arg1	components					999:1008	five 3-O-sulfated components	981:1008	five 3-O-sulfated components	981:1008	We previously examined the reaction products of human 3OST isoforms and identified five 3-O-sulfated components, including three non-AT-binding disaccharides and two AT-binding tetrasaccharides, as digestion products of heparin lyases.
33234593	6	5	theme	3-O-sulfated	986:997	arg1	disaccharides					1042:1054	three non-AT-binding disaccharides	1021:1054	three non-AT-binding disaccharides	1021:1054	We previously examined the reaction products of human 3OST isoforms and identified five 3-O-sulfated components, including three non-AT-binding disaccharides and two AT-binding tetrasaccharides, as digestion products of heparin lyases.
33234593	6	5	theme	3-O-sulfated	986:997	arg1	tetrasaccharides					1075:1090	two AT-binding tetrasaccharides	1060:1090	two AT-binding tetrasaccharides	1060:1090	We previously examined the reaction products of human 3OST isoforms and identified five 3-O-sulfated components, including three non-AT-binding disaccharides and two AT-binding tetrasaccharides, as digestion products of heparin lyases.
33234593	3	6	theme	such	386:389	arg1	site					464:467	a binding site	454:467	a binding site for antithrombin (AT)	454:489	One such motif synthesized by 3-O-sulfotransferase-1 (3OST-1) serves as a binding site for antithrombin (AT) and has been thoroughly studied because of its pharmacological importance.
33234593	3	6	theme	such	386:389	arg1	3OST-1					436:441	3OST-1	436:441	3OST-1	436:441	One such motif synthesized by 3-O-sulfotransferase-1 (3OST-1) serves as a binding site for antithrombin (AT) and has been thoroughly studied because of its pharmacological importance.
33234593	3	6	theme	such	386:389	arg1	motif					391:395	One such motif	382:395	One such motif synthesized by 3-O-sulfotransferase-1 (3OST-1)	382:442	One such motif synthesized by 3-O-sulfotransferase-1 (3OST-1) serves as a binding site for antithrombin (AT) and has been thoroughly studied because of its pharmacological importance.
33234593	4	7	theme	standard	715:722	arg1	method					724:729	a standard method	713:729	a standard method to analyze this rare modification	713:763	However, the physiological roles of 3-O-sulfates produced by other 3OST isoforms, which do not bind AT, remain obscure, in part due to the lack of a standard method to analyze this rare modification.
33234593	5	8	theme	non-AT-binding	876:889	arg1	units					891:895	non-AT-binding units	876:895	non-AT-binding units	876:895	This study aims to establish a method for quantifying 3-O-sulfated components of heparan sulfate, focusing on non-AT-binding units.
33234593	7	9	theme	standard	1188:1195	arg1	components					1172:1181	these five components	1161:1181	these five components	1161:1181	In this study, we prepared these five components as a standard saccharide for HPLC analysis.
33234593	7	9	theme	standard	1188:1195	arg1	saccharide					1197:1206	a standard saccharide	1186:1206	a standard saccharide for HPLC analysis	1186:1224	In this study, we prepared these five components as a standard saccharide for HPLC analysis.
33234593	12	10	theme	new	1836:1838	arg1	approach					1840:1847	a new approach	1834:1847	a new approach to explore the physiological functions of 3-O-sulfate	1834:1901	This is the first report that shows the quantification of three non-AT-binding 3-O-sulfated units and establishes a new approach to explore the physiological functions of 3-O-sulfate.
33234593	3	11	theme	pharmacological	538:552	arg1	importance					554:563	its pharmacological importance	534:563	its pharmacological importance	534:563	One such motif synthesized by 3-O-sulfotransferase-1 (3OST-1) serves as a binding site for antithrombin (AT) and has been thoroughly studied because of its pharmacological importance.
33234593	5	12	theme	sulfate	855:861	arg1	components					833:842	3-O-sulfated components	820:842	3-O-sulfated components of heparan sulfate	820:861	This study aims to establish a method for quantifying 3-O-sulfated components of heparan sulfate, focusing on non-AT-binding units.
33234593	6	13	theme	digestion	1096:1104	arg1	products					1106:1113	digestion products	1096:1113	digestion products of heparin lyases	1096:1131	We previously examined the reaction products of human 3OST isoforms and identified five 3-O-sulfated components, including three non-AT-binding disaccharides and two AT-binding tetrasaccharides, as digestion products of heparin lyases.
33234593	6	13	theme	digestion	1096:1104	arg1	components					999:1008	five 3-O-sulfated components	981:1008	five 3-O-sulfated components	981:1008	We previously examined the reaction products of human 3OST isoforms and identified five 3-O-sulfated components, including three non-AT-binding disaccharides and two AT-binding tetrasaccharides, as digestion products of heparin lyases.
33234593	6	13	theme	digestion	1096:1104	arg1	disaccharides					1042:1054	three non-AT-binding disaccharides	1021:1054	three non-AT-binding disaccharides	1021:1054	We previously examined the reaction products of human 3OST isoforms and identified five 3-O-sulfated components, including three non-AT-binding disaccharides and two AT-binding tetrasaccharides, as digestion products of heparin lyases.
33234593	6	13	theme	digestion	1096:1104	arg1	tetrasaccharides					1075:1090	two AT-binding tetrasaccharides	1060:1090	two AT-binding tetrasaccharides	1060:1090	We previously examined the reaction products of human 3OST isoforms and identified five 3-O-sulfated components, including three non-AT-binding disaccharides and two AT-binding tetrasaccharides, as digestion products of heparin lyases.
33234593	9	14	theme	reverse-phase	1330:1342	arg1	HPLC					1353:1356	reverse-phase ion-pair HPLC	1330:1356	reverse-phase ion-pair HPLC with a postcolumn fluorescent labeling system	1330:1402	Using reverse-phase ion-pair HPLC with a postcolumn fluorescent labeling system, the separation conditions were optimized to quantify the 13 units.
33234593	12	15	theme	physiological	1864:1876	arg1	functions					1878:1886	the physiological functions	1860:1886	the physiological functions of 3-O-sulfate	1860:1901	This is the first report that shows the quantification of three non-AT-binding 3-O-sulfated units and establishes a new approach to explore the physiological functions of 3-O-sulfate.
33234593	12	16	theme	3-O-sulfated	1799:1810	arg1	units					1812:1816	three non-AT-binding 3-O-sulfated units	1778:1816	three non-AT-binding 3-O-sulfated units	1778:1816	This is the first report that shows the quantification of three non-AT-binding 3-O-sulfated units and establishes a new approach to explore the physiological functions of 3-O-sulfate.
33234593	4	17	theme	rare	747:750	arg1	modification					752:763	this rare modification	742:763	this rare modification	742:763	However, the physiological roles of 3-O-sulfates produced by other 3OST isoforms, which do not bind AT, remain obscure, in part due to the lack of a standard method to analyze this rare modification.
33234593	6	18	theme	human	946:950	arg1	isoforms					957:964	human 3OST isoforms	946:964	human 3OST isoforms	946:964	We previously examined the reaction products of human 3OST isoforms and identified five 3-O-sulfated components, including three non-AT-binding disaccharides and two AT-binding tetrasaccharides, as digestion products of heparin lyases.
33234593	5	19	theme	heparan	847:853	arg1	sulfate					855:861	heparan sulfate	847:861	heparan sulfate	847:861	This study aims to establish a method for quantifying 3-O-sulfated components of heparan sulfate, focusing on non-AT-binding units.
33234593	0	20	theme	quantitative	2:13	arg1	method					15:20	A quantitative method	0:20	A quantitative method	0:20	A quantitative method to detect non-antithrombin-binding 3-O-sulfated units in heparan sulfate.
33234593	4	21	theme	physiological	579:591	arg1	roles					593:597	the physiological roles	575:597	the physiological roles	575:597	However, the physiological roles of 3-O-sulfates produced by other 3OST isoforms, which do not bind AT, remain obscure, in part due to the lack of a standard method to analyze this rare modification.
33234593	12	22	theme	non-AT-binding	1784:1797	arg1	units					1812:1816	three non-AT-binding 3-O-sulfated units	1778:1816	three non-AT-binding 3-O-sulfated units	1778:1816	This is the first report that shows the quantification of three non-AT-binding 3-O-sulfated units and establishes a new approach to explore the physiological functions of 3-O-sulfate.
33234593	1	23	theme	Heparan	96:102	arg1	sulfate					104:110	Heparan sulfate	96:110	Heparan sulfate	96:110	Heparan sulfate is synthesized by most animal cells and interacts with numerous proteins via specific sulfation motifs to regulate various physiological processes.
33234593	1	24	theme	specific	189:196	arg1	motifs					208:213	specific sulfation motifs	189:213	specific sulfation motifs	189:213	Heparan sulfate is synthesized by most animal cells and interacts with numerous proteins via specific sulfation motifs to regulate various physiological processes.
33234593	9	25	theme	ion-pair	1344:1351	arg1	HPLC					1353:1356	reverse-phase ion-pair HPLC	1330:1356	reverse-phase ion-pair HPLC with a postcolumn fluorescent labeling system	1330:1402	Using reverse-phase ion-pair HPLC with a postcolumn fluorescent labeling system, the separation conditions were optimized to quantify the 13 units.
33234593	8	26	theme	non-3-O-sulfated	1247:1262	arg1	disaccharides					1264:1276	eight non-3-O-sulfated disaccharides	1241:1276	eight non-3-O-sulfated disaccharides	1241:1276	Together with eight non-3-O-sulfated disaccharides, a standard mixture of 13 units was prepared.
33234593	9	27	with	HPLC	1353:1356	arg1	system					1397:1402	a postcolumn fluorescent labeling system	1363:1402	a postcolumn fluorescent labeling system	1363:1402	Using reverse-phase ion-pair HPLC with a postcolumn fluorescent labeling system, the separation conditions were optimized to quantify the 13 units.
33234593	2	28	theme	binding	332:338	arg1	specificities					340:352	the binding specificities	328:352	the binding specificities to the functional proteins	328:379	Various 3-O-sulfated motifs are considered to be key in controlling the binding specificities to the functional proteins.
33234593	3	29	theme	binding	456:462	arg1	site					464:467	a binding site	454:467	a binding site for antithrombin (AT)	454:489	One such motif synthesized by 3-O-sulfotransferase-1 (3OST-1) serves as a binding site for antithrombin (AT) and has been thoroughly studied because of its pharmacological importance.
33234593	3	29	theme	binding	456:462	arg1	motif					391:395	One such motif	382:395	One such motif synthesized by 3-O-sulfotransferase-1 (3OST-1)	382:442	One such motif synthesized by 3-O-sulfotransferase-1 (3OST-1) serves as a binding site for antithrombin (AT) and has been thoroughly studied because of its pharmacological importance.
33234593	6	30	theme	isoforms	957:964	arg1	products					934:941	the reaction products	921:941	the reaction products of human 3OST isoforms	921:964	We previously examined the reaction products of human 3OST isoforms and identified five 3-O-sulfated components, including three non-AT-binding disaccharides and two AT-binding tetrasaccharides, as digestion products of heparin lyases.
33234593	10	31	theme	3-O-sulfated	1522:1533	arg1	units					1535:1539	3-O-sulfated units	1522:1539	3-O-sulfated units	1522:1539	Finally, we analyzed the compositional changes of 3-O-sulfated units in heparan sulfate from P19 cells before and after neuronal differentiation.
33234593	12	32	theme	units	1812:1816	arg1	quantification					1760:1773	the quantification	1756:1773	the quantification of three non-AT-binding 3-O-sulfated units	1756:1816	This is the first report that shows the quantification of three non-AT-binding 3-O-sulfated units and establishes a new approach to explore the physiological functions of 3-O-sulfate.
33234593	6	33	theme	AT-binding	1064:1073	arg1	tetrasaccharides					1075:1090	two AT-binding tetrasaccharides	1060:1090	two AT-binding tetrasaccharides	1060:1090	We previously examined the reaction products of human 3OST isoforms and identified five 3-O-sulfated components, including three non-AT-binding disaccharides and two AT-binding tetrasaccharides, as digestion products of heparin lyases.
33234593	6	34	theme	3OST	952:955	arg1	isoforms					957:964	human 3OST isoforms	946:964	human 3OST isoforms	946:964	We previously examined the reaction products of human 3OST isoforms and identified five 3-O-sulfated components, including three non-AT-binding disaccharides and two AT-binding tetrasaccharides, as digestion products of heparin lyases.
33234593	10	35	from	P19 cells	1565:1573	arg1	changes					1511:1517	the compositional changes	1493:1517	the compositional changes of 3-O-sulfated units in heparan sulfate from P19 cells	1493:1573	Finally, we analyzed the compositional changes of 3-O-sulfated units in heparan sulfate from P19 cells before and after neuronal differentiation.
33234593	10	35	from	P19 cells	1565:1573	arg1	sulfate					1552:1558	heparan sulfate	1544:1558	heparan sulfate from P19 cells	1544:1573	Finally, we analyzed the compositional changes of 3-O-sulfated units in heparan sulfate from P19 cells before and after neuronal differentiation.
33234593	4	36	theme	method	724:729	arg1	lack					705:708	the lack	701:708	the lack of a standard method to analyze this rare modification	701:763	However, the physiological roles of 3-O-sulfates produced by other 3OST isoforms, which do not bind AT, remain obscure, in part due to the lack of a standard method to analyze this rare modification.
33234593	0	37	theme	non-antithrombin-binding	32:55	arg1	units					70:74	non-antithrombin-binding 3-O-sulfated units	32:74	non-antithrombin-binding 3-O-sulfated units	32:74	A quantitative method to detect non-antithrombin-binding 3-O-sulfated units in heparan sulfate.
33234593	2	38	theme	functional	361:370	arg1	proteins					372:379	the functional proteins	357:379	the functional proteins	357:379	Various 3-O-sulfated motifs are considered to be key in controlling the binding specificities to the functional proteins.
33234593	2	39	theme	Various	260:266	arg1	motifs					281:286	Various 3-O-sulfated motifs	260:286	Various 3-O-sulfated motifs	260:286	Various 3-O-sulfated motifs are considered to be key in controlling the binding specificities to the functional proteins.
33234593	1	40	theme	sulfation	198:206	arg1	motifs					208:213	specific sulfation motifs	189:213	specific sulfation motifs	189:213	Heparan sulfate is synthesized by most animal cells and interacts with numerous proteins via specific sulfation motifs to regulate various physiological processes.
33234593	5	41	theme	3-O-sulfated	820:831	arg1	components					833:842	3-O-sulfated components	820:842	3-O-sulfated components of heparan sulfate	820:861	This study aims to establish a method for quantifying 3-O-sulfated components of heparan sulfate, focusing on non-AT-binding units.
33234593	6	42	theme	non-AT-binding	1027:1040	arg1	disaccharides					1042:1054	three non-AT-binding disaccharides	1021:1054	three non-AT-binding disaccharides	1021:1054	We previously examined the reaction products of human 3OST isoforms and identified five 3-O-sulfated components, including three non-AT-binding disaccharides and two AT-binding tetrasaccharides, as digestion products of heparin lyases.
33234593	9	43	theme	fluorescent	1376:1386	arg1	system					1397:1402	a postcolumn fluorescent labeling system	1363:1402	a postcolumn fluorescent labeling system	1363:1402	Using reverse-phase ion-pair HPLC with a postcolumn fluorescent labeling system, the separation conditions were optimized to quantify the 13 units.
33234593	10	44	theme	compositional	1497:1509	arg1	changes					1511:1517	the compositional changes	1493:1517	the compositional changes of 3-O-sulfated units in heparan sulfate from P19 cells	1493:1573	Finally, we analyzed the compositional changes of 3-O-sulfated units in heparan sulfate from P19 cells before and after neuronal differentiation.
33234593	1	45	theme	various	227:233	arg1	processes					249:257	various physiological processes	227:257	various physiological processes	227:257	Heparan sulfate is synthesized by most animal cells and interacts with numerous proteins via specific sulfation motifs to regulate various physiological processes.
33234593	9	46	theme	labeling	1388:1395	arg1	system					1397:1402	a postcolumn fluorescent labeling system	1363:1402	a postcolumn fluorescent labeling system	1363:1402	Using reverse-phase ion-pair HPLC with a postcolumn fluorescent labeling system, the separation conditions were optimized to quantify the 13 units.
33234593	8	47	theme	units	1304:1308	arg1	mixture					1290:1296	a standard mixture	1279:1296	a standard mixture of 13 units	1279:1308	Together with eight non-3-O-sulfated disaccharides, a standard mixture of 13 units was prepared.
33234593	0	48	theme	3-O-sulfated	57:68	arg1	units					70:74	non-antithrombin-binding 3-O-sulfated units	32:74	non-antithrombin-binding 3-O-sulfated units	32:74	A quantitative method to detect non-antithrombin-binding 3-O-sulfated units in heparan sulfate.
33234593	6	49	theme	reaction	925:932	arg1	products					934:941	the reaction products	921:941	the reaction products of human 3OST isoforms	921:964	We previously examined the reaction products of human 3OST isoforms and identified five 3-O-sulfated components, including three non-AT-binding disaccharides and two AT-binding tetrasaccharides, as digestion products of heparin lyases.
33234593	10	50	theme	heparan	1544:1550	arg1	sulfate					1552:1558	heparan sulfate	1544:1558	heparan sulfate from P19 cells	1544:1573	Finally, we analyzed the compositional changes of 3-O-sulfated units in heparan sulfate from P19 cells before and after neuronal differentiation.
33234593	1	51	theme	most	130:133	arg1	cells					142:146	most animal cells	130:146	most animal cells	130:146	Heparan sulfate is synthesized by most animal cells and interacts with numerous proteins via specific sulfation motifs to regulate various physiological processes.
33234593	10	52	from	changes	1511:1517	arg1	P19 cells					1565:1573	P19 cells	1565:1573	P19 cells	1565:1573	Finally, we analyzed the compositional changes of 3-O-sulfated units in heparan sulfate from P19 cells before and after neuronal differentiation.
33234593	10	52	from	changes	1511:1517	arg1	sulfate					1552:1558	heparan sulfate	1544:1558	heparan sulfate from P19 cells	1544:1573	Finally, we analyzed the compositional changes of 3-O-sulfated units in heparan sulfate from P19 cells before and after neuronal differentiation.
33234593	9	53	theme	postcolumn	1365:1374	arg1	system					1397:1402	a postcolumn fluorescent labeling system	1363:1402	a postcolumn fluorescent labeling system	1363:1402	Using reverse-phase ion-pair HPLC with a postcolumn fluorescent labeling system, the separation conditions were optimized to quantify the 13 units.
33234593	12	54	theme	3-O-sulfate	1891:1901	arg1	functions					1878:1886	the physiological functions	1860:1886	the physiological functions of 3-O-sulfate	1860:1901	This is the first report that shows the quantification of three non-AT-binding 3-O-sulfated units and establishes a new approach to explore the physiological functions of 3-O-sulfate.
33234593	1	55	theme	animal	135:140	arg1	cells					142:146	most animal cells	130:146	most animal cells	130:146	Heparan sulfate is synthesized by most animal cells and interacts with numerous proteins via specific sulfation motifs to regulate various physiological processes.
33234593	10	56	theme	neuronal	1592:1599	arg1	differentiation					1601:1615	neuronal differentiation	1592:1615	neuronal differentiation	1592:1615	Finally, we analyzed the compositional changes of 3-O-sulfated units in heparan sulfate from P19 cells before and after neuronal differentiation.
33234593	4	57	theme	3OST	633:636	arg1	isoforms					638:645	other 3OST isoforms	627:645	other 3OST isoforms	627:645	However, the physiological roles of 3-O-sulfates produced by other 3OST isoforms, which do not bind AT, remain obscure, in part due to the lack of a standard method to analyze this rare modification.
33234593	10	58	theme	units	1535:1539	arg1	changes					1511:1517	the compositional changes	1493:1517	the compositional changes of 3-O-sulfated units in heparan sulfate from P19 cells	1493:1573	Finally, we analyzed the compositional changes of 3-O-sulfated units in heparan sulfate from P19 cells before and after neuronal differentiation.
33234593	6	59	theme	heparin	1118:1124	arg1	lyases					1126:1131	heparin lyases	1118:1131	heparin lyases	1118:1131	We previously examined the reaction products of human 3OST isoforms and identified five 3-O-sulfated components, including three non-AT-binding disaccharides and two AT-binding tetrasaccharides, as digestion products of heparin lyases.
33234593	4	60	theme	other	627:631	arg1	isoforms					638:645	other 3OST isoforms	627:645	other 3OST isoforms	627:645	However, the physiological roles of 3-O-sulfates produced by other 3OST isoforms, which do not bind AT, remain obscure, in part due to the lack of a standard method to analyze this rare modification.
33234593	0	61	theme	heparan	79:85	arg1	sulfate					87:93	heparan sulfate	79:93	heparan sulfate	79:93	A quantitative method to detect non-antithrombin-binding 3-O-sulfated units in heparan sulfate.
33234593	8	62	theme	standard	1281:1288	arg1	mixture					1290:1296	a standard mixture	1279:1296	a standard mixture of 13 units	1279:1308	Together with eight non-3-O-sulfated disaccharides, a standard mixture of 13 units was prepared.
33234593	6	63	theme	lyases	1126:1131	arg1	products					1106:1113	digestion products	1096:1113	digestion products of heparin lyases	1096:1131	We previously examined the reaction products of human 3OST isoforms and identified five 3-O-sulfated components, including three non-AT-binding disaccharides and two AT-binding tetrasaccharides, as digestion products of heparin lyases.
33234593	6	63	theme	lyases	1126:1131	arg1	components					999:1008	five 3-O-sulfated components	981:1008	five 3-O-sulfated components	981:1008	We previously examined the reaction products of human 3OST isoforms and identified five 3-O-sulfated components, including three non-AT-binding disaccharides and two AT-binding tetrasaccharides, as digestion products of heparin lyases.
33234593	6	63	theme	lyases	1126:1131	arg1	disaccharides					1042:1054	three non-AT-binding disaccharides	1021:1054	three non-AT-binding disaccharides	1021:1054	We previously examined the reaction products of human 3OST isoforms and identified five 3-O-sulfated components, including three non-AT-binding disaccharides and two AT-binding tetrasaccharides, as digestion products of heparin lyases.
33234593	6	63	theme	lyases	1126:1131	arg1	tetrasaccharides					1075:1090	two AT-binding tetrasaccharides	1060:1090	two AT-binding tetrasaccharides	1060:1090	We previously examined the reaction products of human 3OST isoforms and identified five 3-O-sulfated components, including three non-AT-binding disaccharides and two AT-binding tetrasaccharides, as digestion products of heparin lyases.
33234593	11	64	theme	differentiated	1696:1709	arg1	neurons					1711:1717	the differentiated neurons	1692:1717	the differentiated neurons	1692:1717	We successfully detected the 3-O-sulfated units specifically expressed in the differentiated neurons.
33234593	2	65	theme	3-O-sulfated	268:279	arg1	motifs					281:286	Various 3-O-sulfated motifs	260:286	Various 3-O-sulfated motifs	260:286	Various 3-O-sulfated motifs are considered to be key in controlling the binding specificities to the functional proteins.
33234593	11	66	theme	3-O-sulfated	1647:1658	arg1	units					1660:1664	the 3-O-sulfated units	1643:1664	the 3-O-sulfated units specifically expressed in the differentiated neurons	1643:1717	We successfully detected the 3-O-sulfated units specifically expressed in the differentiated neurons.
33234593	1	67	theme	physiological	235:247	arg1	processes					249:257	various physiological processes	227:257	various physiological processes	227:257	Heparan sulfate is synthesized by most animal cells and interacts with numerous proteins via specific sulfation motifs to regulate various physiological processes.
33234593	7	68	theme	HPLC	1212:1215	arg1	analysis					1217:1224	HPLC analysis	1212:1224	HPLC analysis	1212:1224	In this study, we prepared these five components as a standard saccharide for HPLC analysis.
33288190	2	0	theme	oat	518:520	arg1	β-glucan					522:529	processed oat β-glucan	508:529	processed oat β-glucan	508:529	The results showed that microwave-processed oat β-glucan with lower average molecular weight (Mw) exhibited a more significant effect in promoting Lactobacillus and Bifidobacterium compared with steaming processed oat β-glucan with higher Mw at the genus level.
33288190	0	1	dep	in	119:120	arg1	vitro					122:126	vitro	122:126	vitro	122:126	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.
33288190	4	2	theme	microwave-processed	961:979	arg1	β-glucan					985:992	microwave-processed oat β-glucan	961:992	microwave-processed oat β-glucan	961:992	Additionally, the short-chain fatty acid in microwave-processed oat β-glucan fermentation slurries significantly increased (p < 0.05), and more butyrate was produced from microwave-processed oat β-glucan, which may be attributed to the higher levels of Blautia and Dialister as butyrate-producers.
33288190	0	3	theme	prebiotic	78:86	arg1	effects					88:94	prebiotic effects	78:94	prebiotic effects	78:94	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.
33288190	0	4	from	study	12:16	arg1	properties					63:72	physicochemical properties	47:72	physicochemical properties	47:72	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.
33288190	0	4	from	study	12:16	arg1	effects					88:94	prebiotic effects	78:94	prebiotic effects	78:94	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.
33288190	2	5	theme	lower	366:370	arg1	Mw					398:399	Mw	398:399	Mw	398:399	The results showed that microwave-processed oat β-glucan with lower average molecular weight (Mw) exhibited a more significant effect in promoting Lactobacillus and Bifidobacterium compared with steaming processed oat β-glucan with higher Mw at the genus level.
33288190	2	5	theme	lower	366:370	arg1	weight					390:395	lower average molecular weight (Mw)	366:400	lower average molecular weight (Mw)	366:400	The results showed that microwave-processed oat β-glucan with lower average molecular weight (Mw) exhibited a more significant effect in promoting Lactobacillus and Bifidobacterium compared with steaming processed oat β-glucan with higher Mw at the genus level.
33288190	5	6	theme	β-glucan	1174:1181	arg1	degradation					1155:1165	the degradation	1151:1165	the degradation of oat β-glucan	1151:1181	These results suggest that microwave processing contributed to the degradation of oat β-glucan and enhanced its prebiotic function.
33288190	4	7	theme	microwave-processed	834:852	arg1	slurries					880:887	microwave-processed oat β-glucan fermentation slurries	834:887	microwave-processed oat β-glucan fermentation slurries	834:887	Additionally, the short-chain fatty acid in microwave-processed oat β-glucan fermentation slurries significantly increased (p < 0.05), and more butyrate was produced from microwave-processed oat β-glucan, which may be attributed to the higher levels of Blautia and Dialister as butyrate-producers.
33288190	4	8	from	acid	826:829	arg1	slurries					880:887	microwave-processed oat β-glucan fermentation slurries	834:887	microwave-processed oat β-glucan fermentation slurries	834:887	Additionally, the short-chain fatty acid in microwave-processed oat β-glucan fermentation slurries significantly increased (p < 0.05), and more butyrate was produced from microwave-processed oat β-glucan, which may be attributed to the higher levels of Blautia and Dialister as butyrate-producers.
33288190	4	9	theme	fatty	820:824	arg1	acid					826:829	the short-chain fatty acid	804:829	the short-chain fatty acid in microwave-processed oat β-glucan fermentation slurries	804:887	Additionally, the short-chain fatty acid in microwave-processed oat β-glucan fermentation slurries significantly increased (p < 0.05), and more butyrate was produced from microwave-processed oat β-glucan, which may be attributed to the higher levels of Blautia and Dialister as butyrate-producers.
33288190	3	10	theme	microbial	578:586	arg1	results					610:616	The overall microbial composition structure results	566:616	The overall microbial composition structure results	566:616	The overall microbial composition structure results indicated that the relative abundance of Escherichia-Shigella, Lactobacillus, Enterococcus, and Dialister exhibited significant differences between the samples (p < 0.05).
33288190	1	11	theme	microwave	252:260	arg1	processing					262:271	microwave processing	252:271	microwave processing	252:271	This study aimed to compare the prebiotic effects of oat β-glucan between steaming and microwave processing by high-throughput sequencing.
33288190	3	12	theme	significant	734:744	arg1	differences					746:756	significant differences	734:756	significant differences between the samples (p < 0.05)	734:787	The overall microbial composition structure results indicated that the relative abundance of Escherichia-Shigella, Lactobacillus, Enterococcus, and Dialister exhibited significant differences between the samples (p < 0.05).
33288190	4	13	theme	short-chain	808:818	arg1	acid					826:829	the short-chain fatty acid	804:829	the short-chain fatty acid in microwave-processed oat β-glucan fermentation slurries	804:887	Additionally, the short-chain fatty acid in microwave-processed oat β-glucan fermentation slurries significantly increased (p < 0.05), and more butyrate was produced from microwave-processed oat β-glucan, which may be attributed to the higher levels of Blautia and Dialister as butyrate-producers.
33288190	2	14	theme	average	372:378	arg1	Mw					398:399	Mw	398:399	Mw	398:399	The results showed that microwave-processed oat β-glucan with lower average molecular weight (Mw) exhibited a more significant effect in promoting Lactobacillus and Bifidobacterium compared with steaming processed oat β-glucan with higher Mw at the genus level.
33288190	2	14	theme	average	372:378	arg1	weight					390:395	lower average molecular weight (Mw)	366:400	lower average molecular weight (Mw)	366:400	The results showed that microwave-processed oat β-glucan with lower average molecular weight (Mw) exhibited a more significant effect in promoting Lactobacillus and Bifidobacterium compared with steaming processed oat β-glucan with higher Mw at the genus level.
33288190	4	15	theme	Dialister	1055:1063	arg1	levels					1033:1038	the higher levels	1022:1038	the higher levels of Blautia and Dialister as butyrate-producers	1022:1085	Additionally, the short-chain fatty acid in microwave-processed oat β-glucan fermentation slurries significantly increased (p < 0.05), and more butyrate was produced from microwave-processed oat β-glucan, which may be attributed to the higher levels of Blautia and Dialister as butyrate-producers.
33288190	0	16	theme	β-glucan	107:114	arg1	properties					63:72	physicochemical properties	47:72	physicochemical properties	47:72	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.
33288190	0	16	theme	β-glucan	107:114	arg1	effects					88:94	prebiotic effects	78:94	prebiotic effects	78:94	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.
33288190	4	17	dep	increased	903:911	arg1	p < 0.05					914:921	p < 0.05	914:921	p < 0.05	914:921	Additionally, the short-chain fatty acid in microwave-processed oat β-glucan fermentation slurries significantly increased (p < 0.05), and more butyrate was produced from microwave-processed oat β-glucan, which may be attributed to the higher levels of Blautia and Dialister as butyrate-producers.
33288190	5	18	theme	oat	1170:1172	arg1	β-glucan					1174:1181	oat β-glucan	1170:1181	oat β-glucan	1170:1181	These results suggest that microwave processing contributed to the degradation of oat β-glucan and enhanced its prebiotic function.
33288190	0	19	theme	oat	103:105	arg1	β-glucan					107:114	the oat β-glucan	99:114	the oat β-glucan	99:114	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.
33288190	2	20	theme	processed	508:516	arg1	β-glucan					522:529	processed oat β-glucan	508:529	processed oat β-glucan	508:529	The results showed that microwave-processed oat β-glucan with lower average molecular weight (Mw) exhibited a more significant effect in promoting Lactobacillus and Bifidobacterium compared with steaming processed oat β-glucan with higher Mw at the genus level.
33288190	4	21	theme	fermentation	867:878	arg1	slurries					880:887	microwave-processed oat β-glucan fermentation slurries	834:887	microwave-processed oat β-glucan fermentation slurries	834:887	Additionally, the short-chain fatty acid in microwave-processed oat β-glucan fermentation slurries significantly increased (p < 0.05), and more butyrate was produced from microwave-processed oat β-glucan, which may be attributed to the higher levels of Blautia and Dialister as butyrate-producers.
33288190	4	22	theme	higher	1026:1031	arg1	levels					1033:1038	the higher levels	1022:1038	the higher levels of Blautia and Dialister as butyrate-producers	1022:1085	Additionally, the short-chain fatty acid in microwave-processed oat β-glucan fermentation slurries significantly increased (p < 0.05), and more butyrate was produced from microwave-processed oat β-glucan, which may be attributed to the higher levels of Blautia and Dialister as butyrate-producers.
33288190	0	23	from	processing	29:38	arg1	properties					63:72	physicochemical properties	47:72	physicochemical properties	47:72	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.
33288190	0	23	from	processing	29:38	arg1	effects					88:94	prebiotic effects	78:94	prebiotic effects	78:94	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.
33288190	3	24	theme	composition	588:598	arg1	results					610:616	The overall microbial composition structure results	566:616	The overall microbial composition structure results	566:616	The overall microbial composition structure results indicated that the relative abundance of Escherichia-Shigella, Lactobacillus, Enterococcus, and Dialister exhibited significant differences between the samples (p < 0.05).
33288190	0	25	theme	Comparative	0:10	arg1	study					12:16	Comparative study	0:16	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.	0:163	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.
33288190	3	26	theme	structure	600:608	arg1	results					610:616	The overall microbial composition structure results	566:616	The overall microbial composition structure results	566:616	The overall microbial composition structure results indicated that the relative abundance of Escherichia-Shigella, Lactobacillus, Enterococcus, and Dialister exhibited significant differences between the samples (p < 0.05).
33288190	0	27	theme	thermal	21:27	arg1	processing					29:38	thermal processing	21:38	thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan	21:114	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.
33288190	1	28	theme	high-throughput	276:290	arg1	sequencing					292:301	high-throughput sequencing	276:301	high-throughput sequencing	276:301	This study aimed to compare the prebiotic effects of oat β-glucan between steaming and microwave processing by high-throughput sequencing.
33288190	0	29	theme	in	119:120	arg1	fermentation					151:162	in vitro human fecal microbiota fermentation	119:162	in vitro human fecal microbiota fermentation	119:162	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.
33288190	3	30	theme	overall	570:576	arg1	results					610:616	The overall microbial composition structure results	566:616	The overall microbial composition structure results	566:616	The overall microbial composition structure results indicated that the relative abundance of Escherichia-Shigella, Lactobacillus, Enterococcus, and Dialister exhibited significant differences between the samples (p < 0.05).
33288190	3	31	theme	Lactobacillus	681:693	arg1	abundance					646:654	the relative abundance	633:654	the relative abundance of Escherichia-Shigella, Lactobacillus, Enterococcus, and Dialister	633:722	The overall microbial composition structure results indicated that the relative abundance of Escherichia-Shigella, Lactobacillus, Enterococcus, and Dialister exhibited significant differences between the samples (p < 0.05).
33288190	0	32	from	effects	88:94	arg1	study					12:16	Comparative study	0:16	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.	0:163	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.
33288190	0	33	dep	properties	63:72	arg1	the					43:45	the	43:45	the	43:45	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.
33288190	2	34	theme	significant	419:429	arg1	effect					431:436	a more significant effect	412:436	a more significant effect	412:436	The results showed that microwave-processed oat β-glucan with lower average molecular weight (Mw) exhibited a more significant effect in promoting Lactobacillus and Bifidobacterium compared with steaming processed oat β-glucan with higher Mw at the genus level.
33288190	0	35	theme	human	128:132	arg1	fermentation					151:162	in vitro human fecal microbiota fermentation	119:162	in vitro human fecal microbiota fermentation	119:162	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.
33288190	3	36	theme	Enterococcus	696:707	arg1	abundance					646:654	the relative abundance	633:654	the relative abundance of Escherichia-Shigella, Lactobacillus, Enterococcus, and Dialister	633:722	The overall microbial composition structure results indicated that the relative abundance of Escherichia-Shigella, Lactobacillus, Enterococcus, and Dialister exhibited significant differences between the samples (p < 0.05).
33288190	5	37	theme	microwave	1115:1123	arg1	processing					1125:1134	microwave processing	1115:1134	microwave processing	1115:1134	These results suggest that microwave processing contributed to the degradation of oat β-glucan and enhanced its prebiotic function.
33288190	1	38	theme	prebiotic	197:205	arg1	effects					207:213	the prebiotic effects	193:213	the prebiotic effects of oat β-glucan between steaming and microwave processing	193:271	This study aimed to compare the prebiotic effects of oat β-glucan between steaming and microwave processing by high-throughput sequencing.
33288190	0	39	theme	processing	29:38	arg1	study					12:16	Comparative study	0:16	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.	0:163	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.
33288190	4	40	theme	Blautia	1043:1049	arg1	levels					1033:1038	the higher levels	1022:1038	the higher levels of Blautia and Dialister as butyrate-producers	1022:1085	Additionally, the short-chain fatty acid in microwave-processed oat β-glucan fermentation slurries significantly increased (p < 0.05), and more butyrate was produced from microwave-processed oat β-glucan, which may be attributed to the higher levels of Blautia and Dialister as butyrate-producers.
33288190	2	41	theme	genus	553:557	arg1	level					559:563	the genus level	549:563	the genus level	549:563	The results showed that microwave-processed oat β-glucan with lower average molecular weight (Mw) exhibited a more significant effect in promoting Lactobacillus and Bifidobacterium compared with steaming processed oat β-glucan with higher Mw at the genus level.
33288190	2	42	theme	higher	536:541	arg1	Mw					543:544	higher Mw	536:544	higher Mw at the genus level	536:563	The results showed that microwave-processed oat β-glucan with lower average molecular weight (Mw) exhibited a more significant effect in promoting Lactobacillus and Bifidobacterium compared with steaming processed oat β-glucan with higher Mw at the genus level.
33288190	0	43	theme	physicochemical	47:61	arg1	properties					63:72	physicochemical properties	47:72	physicochemical properties	47:72	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.
33288190	0	44	theme	microbiota	140:149	arg1	fermentation					151:162	in vitro human fecal microbiota fermentation	119:162	in vitro human fecal microbiota fermentation	119:162	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.
33288190	0	45	from	properties	63:72	arg1	study					12:16	Comparative study	0:16	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.	0:163	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.
33288190	0	46	theme	fecal	134:138	arg1	fermentation					151:162	in vitro human fecal microbiota fermentation	119:162	in vitro human fecal microbiota fermentation	119:162	Comparative study of thermal processing on the physicochemical properties and prebiotic effects of the oat β-glucan by in vitro human fecal microbiota fermentation.
33288190	3	47	theme	Dialister	714:722	arg1	abundance					646:654	the relative abundance	633:654	the relative abundance of Escherichia-Shigella, Lactobacillus, Enterococcus, and Dialister	633:722	The overall microbial composition structure results indicated that the relative abundance of Escherichia-Shigella, Lactobacillus, Enterococcus, and Dialister exhibited significant differences between the samples (p < 0.05).
33288190	4	48	theme	oat	854:856	arg1	slurries					880:887	microwave-processed oat β-glucan fermentation slurries	834:887	microwave-processed oat β-glucan fermentation slurries	834:887	Additionally, the short-chain fatty acid in microwave-processed oat β-glucan fermentation slurries significantly increased (p < 0.05), and more butyrate was produced from microwave-processed oat β-glucan, which may be attributed to the higher levels of Blautia and Dialister as butyrate-producers.
33288190	2	49	theme	oat	348:350	arg1	β-glucan					352:359	microwave-processed oat β-glucan	328:359	microwave-processed oat β-glucan with lower average molecular weight (Mw)	328:400	The results showed that microwave-processed oat β-glucan with lower average molecular weight (Mw) exhibited a more significant effect in promoting Lactobacillus and Bifidobacterium compared with steaming processed oat β-glucan with higher Mw at the genus level.
33288190	3	50	theme	relative	637:644	arg1	abundance					646:654	the relative abundance	633:654	the relative abundance of Escherichia-Shigella, Lactobacillus, Enterococcus, and Dialister	633:722	The overall microbial composition structure results indicated that the relative abundance of Escherichia-Shigella, Lactobacillus, Enterococcus, and Dialister exhibited significant differences between the samples (p < 0.05).
33288190	2	51	from	level	559:563	arg1	Mw					543:544	higher Mw	536:544	higher Mw at the genus level	536:563	The results showed that microwave-processed oat β-glucan with lower average molecular weight (Mw) exhibited a more significant effect in promoting Lactobacillus and Bifidobacterium compared with steaming processed oat β-glucan with higher Mw at the genus level.
33288190	4	52	theme	oat	981:983	arg1	β-glucan					985:992	microwave-processed oat β-glucan	961:992	microwave-processed oat β-glucan	961:992	Additionally, the short-chain fatty acid in microwave-processed oat β-glucan fermentation slurries significantly increased (p < 0.05), and more butyrate was produced from microwave-processed oat β-glucan, which may be attributed to the higher levels of Blautia and Dialister as butyrate-producers.
33288190	3	53	theme	Escherichia-Shigella	659:678	arg1	abundance					646:654	the relative abundance	633:654	the relative abundance of Escherichia-Shigella, Lactobacillus, Enterococcus, and Dialister	633:722	The overall microbial composition structure results indicated that the relative abundance of Escherichia-Shigella, Lactobacillus, Enterococcus, and Dialister exhibited significant differences between the samples (p < 0.05).
33288190	2	54	theme	microwave-processed	328:346	arg1	β-glucan					352:359	microwave-processed oat β-glucan	328:359	microwave-processed oat β-glucan with lower average molecular weight (Mw)	328:400	The results showed that microwave-processed oat β-glucan with lower average molecular weight (Mw) exhibited a more significant effect in promoting Lactobacillus and Bifidobacterium compared with steaming processed oat β-glucan with higher Mw at the genus level.
33288190	1	55	theme	oat	218:220	arg1	β-glucan					222:229	oat β-glucan	218:229	oat β-glucan	218:229	This study aimed to compare the prebiotic effects of oat β-glucan between steaming and microwave processing by high-throughput sequencing.
33288190	2	56	dep	exhibited	402:410	arg1	compared					485:492	compared	485:492	compared with steaming processed oat β-glucan with higher Mw at the genus level	485:563	The results showed that microwave-processed oat β-glucan with lower average molecular weight (Mw) exhibited a more significant effect in promoting Lactobacillus and Bifidobacterium compared with steaming processed oat β-glucan with higher Mw at the genus level.
33288190	2	57	with	β-glucan	352:359	arg1	Mw					398:399	Mw	398:399	Mw	398:399	The results showed that microwave-processed oat β-glucan with lower average molecular weight (Mw) exhibited a more significant effect in promoting Lactobacillus and Bifidobacterium compared with steaming processed oat β-glucan with higher Mw at the genus level.
33288190	2	57	with	β-glucan	352:359	arg1	weight					390:395	lower average molecular weight (Mw)	366:400	lower average molecular weight (Mw)	366:400	The results showed that microwave-processed oat β-glucan with lower average molecular weight (Mw) exhibited a more significant effect in promoting Lactobacillus and Bifidobacterium compared with steaming processed oat β-glucan with higher Mw at the genus level.
33288190	1	58	theme	β-glucan	222:229	arg1	effects					207:213	the prebiotic effects	193:213	the prebiotic effects of oat β-glucan between steaming and microwave processing	193:271	This study aimed to compare the prebiotic effects of oat β-glucan between steaming and microwave processing by high-throughput sequencing.
33288190	5	59	theme	prebiotic	1200:1208	arg1	function					1210:1217	its prebiotic function	1196:1217	its prebiotic function	1196:1217	These results suggest that microwave processing contributed to the degradation of oat β-glucan and enhanced its prebiotic function.
33288190	4	60	theme	β-glucan	858:865	arg1	slurries					880:887	microwave-processed oat β-glucan fermentation slurries	834:887	microwave-processed oat β-glucan fermentation slurries	834:887	Additionally, the short-chain fatty acid in microwave-processed oat β-glucan fermentation slurries significantly increased (p < 0.05), and more butyrate was produced from microwave-processed oat β-glucan, which may be attributed to the higher levels of Blautia and Dialister as butyrate-producers.
33288190	2	61	theme	molecular	380:388	arg1	Mw					398:399	Mw	398:399	Mw	398:399	The results showed that microwave-processed oat β-glucan with lower average molecular weight (Mw) exhibited a more significant effect in promoting Lactobacillus and Bifidobacterium compared with steaming processed oat β-glucan with higher Mw at the genus level.
33288190	2	61	theme	molecular	380:388	arg1	weight					390:395	lower average molecular weight (Mw)	366:400	lower average molecular weight (Mw)	366:400	The results showed that microwave-processed oat β-glucan with lower average molecular weight (Mw) exhibited a more significant effect in promoting Lactobacillus and Bifidobacterium compared with steaming processed oat β-glucan with higher Mw at the genus level.
32838704	0	0	theme	biomimetic	90:99	arg1	application					101:111	biomimetic application	90:111	biomimetic application	90:111	Fluidic embedding of additional macroporosity in alginate-gelatin composite structure for biomimetic application.
32838704	0	1	from	embedding	8:16	arg1	structure					76:84	alginate-gelatin composite structure	49:84	alginate-gelatin composite structure for biomimetic application	49:111	Fluidic embedding of additional macroporosity in alginate-gelatin composite structure for biomimetic application.
32838704	3	2	theme	breast	680:685	arg1	cells					694:698	MDA MB 231 breast cancer cells	669:698	MDA MB 231 breast cancer cells	669:698	More importantly, voids enabled the cells to penetrate from the surface of seeding into the depth of the scaffold and proliferate there, as demonstrated for MDA MB 231 breast cancer cells.
32838704	4	3	theme	due	858:860	arg1	media					852:856	the media	848:856	the media due to hydrophilicity	848:878	The uniform voids, generated by the microfluidic device, self-align creating uniform macroporosity within the gel structure, get readily filled by the media due to hydrophilicity, and extend the characteristics of composite uniformly across the entire scaffold.
32838704	4	4	theme	uniform	778:784	arg1	macroporosity					786:798	uniform macroporosity	778:798	uniform macroporosity	778:798	The uniform voids, generated by the microfluidic device, self-align creating uniform macroporosity within the gel structure, get readily filled by the media due to hydrophilicity, and extend the characteristics of composite uniformly across the entire scaffold.
32838704	4	5	theme	entire	946:951	arg1	scaffold					953:960	the entire scaffold	942:960	the entire scaffold	942:960	The uniform voids, generated by the microfluidic device, self-align creating uniform macroporosity within the gel structure, get readily filled by the media due to hydrophilicity, and extend the characteristics of composite uniformly across the entire scaffold.
32838704	2	6	theme	polymer	305:311	arg1	networks					313:320	polymer networks	305:320	polymer networks	305:320	Superposition of alginate and gelatin polymer networks results in additional rigidity, which can be tuned by introduction of voids, and thereby allowing faster release of pore pressure through movement of aqueous phase through the pore network.
32838704	2	7	theme	aqueous	472:478	arg1	phase					480:484	aqueous phase	472:484	aqueous phase	472:484	Superposition of alginate and gelatin polymer networks results in additional rigidity, which can be tuned by introduction of voids, and thereby allowing faster release of pore pressure through movement of aqueous phase through the pore network.
32838704	2	8	theme	gelatin	297:303	arg1	Superposition					267:279	Superposition	267:279	Superposition of alginate and gelatin polymer networks	267:320	Superposition of alginate and gelatin polymer networks results in additional rigidity, which can be tuned by introduction of voids, and thereby allowing faster release of pore pressure through movement of aqueous phase through the pore network.
32838704	2	9	theme	pore	498:501	arg1	network					503:509	the pore network	494:509	the pore network	494:509	Superposition of alginate and gelatin polymer networks results in additional rigidity, which can be tuned by introduction of voids, and thereby allowing faster release of pore pressure through movement of aqueous phase through the pore network.
32838704	0	10	theme	Fluidic	0:6	arg1	embedding					8:16	Fluidic embedding	0:16	Fluidic embedding of additional macroporosity in alginate-gelatin composite structure for biomimetic application.	0:112	Fluidic embedding of additional macroporosity in alginate-gelatin composite structure for biomimetic application.
32838704	1	11	theme	Biomimetic	114:123	arg1	characteristics					125:139	Biomimetic characteristics	114:139	Biomimetic characteristics of hydrogel scaffold	114:160	Biomimetic characteristics of hydrogel scaffold are tuned in this study utilizing the synergy of alginate, gelatin, and microfluidically embedded voids.
32838704	1	12	theme	alginate	211:218	arg1	synergy					200:206	the synergy	196:206	the synergy of alginate, gelatin, and microfluidically embedded voids	196:264	Biomimetic characteristics of hydrogel scaffold are tuned in this study utilizing the synergy of alginate, gelatin, and microfluidically embedded voids.
32838704	0	13	theme	additional	21:30	arg1	macroporosity					32:44	additional macroporosity	21:44	additional macroporosity	21:44	Fluidic embedding of additional macroporosity in alginate-gelatin composite structure for biomimetic application.
32838704	2	14	theme	additional	333:342	arg1	rigidity					344:351	additional rigidity	333:351	additional rigidity	333:351	Superposition of alginate and gelatin polymer networks results in additional rigidity, which can be tuned by introduction of voids, and thereby allowing faster release of pore pressure through movement of aqueous phase through the pore network.
32838704	2	15	theme	pore	438:441	arg1	pressure					443:450	pore pressure	438:450	pore pressure	438:450	Superposition of alginate and gelatin polymer networks results in additional rigidity, which can be tuned by introduction of voids, and thereby allowing faster release of pore pressure through movement of aqueous phase through the pore network.
32838704	2	16	theme	pressure	443:450	arg1	release					427:433	faster release	420:433	faster release of pore pressure	420:450	Superposition of alginate and gelatin polymer networks results in additional rigidity, which can be tuned by introduction of voids, and thereby allowing faster release of pore pressure through movement of aqueous phase through the pore network.
32838704	1	17	theme	gelatin	221:227	arg1	synergy					200:206	the synergy	196:206	the synergy of alginate, gelatin, and microfluidically embedded voids	196:264	Biomimetic characteristics of hydrogel scaffold are tuned in this study utilizing the synergy of alginate, gelatin, and microfluidically embedded voids.
32838704	4	18	theme	composite	915:923	arg1	characteristics					896:910	the characteristics	892:910	the characteristics of composite	892:923	The uniform voids, generated by the microfluidic device, self-align creating uniform macroporosity within the gel structure, get readily filled by the media due to hydrophilicity, and extend the characteristics of composite uniformly across the entire scaffold.
32838704	4	19	theme	gel	811:813	arg1	structure					815:823	the gel structure	807:823	the gel structure	807:823	The uniform voids, generated by the microfluidic device, self-align creating uniform macroporosity within the gel structure, get readily filled by the media due to hydrophilicity, and extend the characteristics of composite uniformly across the entire scaffold.
32838704	2	20	dep	alginate	284:291	arg1	networks					313:320	polymer networks	305:320	polymer networks	305:320	Superposition of alginate and gelatin polymer networks results in additional rigidity, which can be tuned by introduction of voids, and thereby allowing faster release of pore pressure through movement of aqueous phase through the pore network.
32838704	0	21	theme	macroporosity	32:44	arg1	embedding					8:16	Fluidic embedding	0:16	Fluidic embedding of additional macroporosity in alginate-gelatin composite structure for biomimetic application.	0:112	Fluidic embedding of additional macroporosity in alginate-gelatin composite structure for biomimetic application.
32838704	4	22	theme	microfluidic	737:748	arg1	device					750:755	the microfluidic device	733:755	the microfluidic device	733:755	The uniform voids, generated by the microfluidic device, self-align creating uniform macroporosity within the gel structure, get readily filled by the media due to hydrophilicity, and extend the characteristics of composite uniformly across the entire scaffold.
32838704	4	22	theme	microfluidic	737:748	arg1	self-align					758:767	self-align	758:767	self-align creating uniform macroporosity within the gel structure	758:823	The uniform voids, generated by the microfluidic device, self-align creating uniform macroporosity within the gel structure, get readily filled by the media due to hydrophilicity, and extend the characteristics of composite uniformly across the entire scaffold.
32838704	2	23	theme	faster	420:425	arg1	release					427:433	faster release	420:433	faster release of pore pressure	420:450	Superposition of alginate and gelatin polymer networks results in additional rigidity, which can be tuned by introduction of voids, and thereby allowing faster release of pore pressure through movement of aqueous phase through the pore network.
32838704	2	24	theme	phase	480:484	arg1	movement					460:467	movement	460:467	movement of aqueous phase through the pore network	460:509	Superposition of alginate and gelatin polymer networks results in additional rigidity, which can be tuned by introduction of voids, and thereby allowing faster release of pore pressure through movement of aqueous phase through the pore network.
32838704	3	25	theme	seeding	587:593	arg1	surface					576:582	the surface	572:582	the surface of seeding	572:593	More importantly, voids enabled the cells to penetrate from the surface of seeding into the depth of the scaffold and proliferate there, as demonstrated for MDA MB 231 breast cancer cells.
32838704	1	26	theme	hydrogel	144:151	arg1	scaffold					153:160	hydrogel scaffold	144:160	hydrogel scaffold	144:160	Biomimetic characteristics of hydrogel scaffold are tuned in this study utilizing the synergy of alginate, gelatin, and microfluidically embedded voids.
32838704	0	27	theme	alginate-gelatin	49:64	arg1	structure					76:84	alginate-gelatin composite structure	49:84	alginate-gelatin composite structure for biomimetic application	49:111	Fluidic embedding of additional macroporosity in alginate-gelatin composite structure for biomimetic application.
32838704	3	28	theme	MDA	669:671	arg1	cells					694:698	MDA MB 231 breast cancer cells	669:698	MDA MB 231 breast cancer cells	669:698	More importantly, voids enabled the cells to penetrate from the surface of seeding into the depth of the scaffold and proliferate there, as demonstrated for MDA MB 231 breast cancer cells.
32838704	1	29	theme	scaffold	153:160	arg1	characteristics					125:139	Biomimetic characteristics	114:139	Biomimetic characteristics of hydrogel scaffold	114:160	Biomimetic characteristics of hydrogel scaffold are tuned in this study utilizing the synergy of alginate, gelatin, and microfluidically embedded voids.
32838704	3	30	theme	MB	673:674	arg1	cells					694:698	MDA MB 231 breast cancer cells	669:698	MDA MB 231 breast cancer cells	669:698	More importantly, voids enabled the cells to penetrate from the surface of seeding into the depth of the scaffold and proliferate there, as demonstrated for MDA MB 231 breast cancer cells.
32838704	3	31	theme	scaffold	617:624	arg1	depth					604:608	the depth	600:608	the depth of the scaffold	600:624	More importantly, voids enabled the cells to penetrate from the surface of seeding into the depth of the scaffold and proliferate there, as demonstrated for MDA MB 231 breast cancer cells.
32838704	2	32	theme	alginate	284:291	arg1	Superposition					267:279	Superposition	267:279	Superposition of alginate and gelatin polymer networks	267:320	Superposition of alginate and gelatin polymer networks results in additional rigidity, which can be tuned by introduction of voids, and thereby allowing faster release of pore pressure through movement of aqueous phase through the pore network.
32838704	4	33	theme	uniform	705:711	arg1	voids					713:717	The uniform voids	701:717	The uniform voids	701:717	The uniform voids, generated by the microfluidic device, self-align creating uniform macroporosity within the gel structure, get readily filled by the media due to hydrophilicity, and extend the characteristics of composite uniformly across the entire scaffold.
32838704	1	34	theme	embedded	251:258	arg1	voids					260:264	microfluidically embedded voids	234:264	microfluidically embedded voids	234:264	Biomimetic characteristics of hydrogel scaffold are tuned in this study utilizing the synergy of alginate, gelatin, and microfluidically embedded voids.
32838704	2	35	theme	voids	392:396	arg1	introduction					376:387	introduction	376:387	introduction of voids	376:396	Superposition of alginate and gelatin polymer networks results in additional rigidity, which can be tuned by introduction of voids, and thereby allowing faster release of pore pressure through movement of aqueous phase through the pore network.
32838704	1	36	theme	voids	260:264	arg1	synergy					200:206	the synergy	196:206	the synergy of alginate, gelatin, and microfluidically embedded voids	196:264	Biomimetic characteristics of hydrogel scaffold are tuned in this study utilizing the synergy of alginate, gelatin, and microfluidically embedded voids.
32838704	0	37	theme	composite	66:74	arg1	structure					76:84	alginate-gelatin composite structure	49:84	alginate-gelatin composite structure for biomimetic application	49:111	Fluidic embedding of additional macroporosity in alginate-gelatin composite structure for biomimetic application.
32838704	3	38	theme	cancer	687:692	arg1	cells					694:698	MDA MB 231 breast cancer cells	669:698	MDA MB 231 breast cancer cells	669:698	More importantly, voids enabled the cells to penetrate from the surface of seeding into the depth of the scaffold and proliferate there, as demonstrated for MDA MB 231 breast cancer cells.
33618089	3	0	theme	thymus	678:683	arg1	indices					685:691	thymus indices	678:691	thymus indices	678:691	In normal BALB/c mice, GCNH at 100-400 mg/(kg d) showed higher immune-promoting activity than CNH via increasing serum IgM, IgA, and IgG by 1.5-24.5%, enhancing spleen and thymus indices by 9.7-26.2%, or increasing splenocyte lymphocyte proliferation and natural killer (NK) cell activity by 1.2-11.5%.
33618089	4	1	theme	higher	827:832	arg1	activity					834:841	higher activity	827:841	higher activity than CNH in the suppressed BALB/c mice	827:880	GCNH also exerted higher activity than CNH in the suppressed BALB/c mice through increasing serum IgM, IgA, and IgG by 2.6-10.5%, enhancing spleen and thymus indices by 0.4-50.1%, or increasing splenocyte lymphocyte proliferation and NK cell activity by 3.4-18.9%.
33618089	5	2	theme	improved	1210:1217	arg1	activity					1236:1243	improved immune-promoting activity	1210:1243	improved immune-promoting activity once hydrolyzed by trypsin	1210:1270	The results highlight that this TGase-type oligochitosan-glycation is potential to generate functional protein ingredients that possess improved immune-promoting activity once hydrolyzed by trypsin.
33618089	4	3	from	activity	834:841	arg1	mice					877:880	the suppressed BALB/c mice	855:880	the suppressed BALB/c mice	855:880	GCNH also exerted higher activity than CNH in the suppressed BALB/c mice through increasing serum IgM, IgA, and IgG by 2.6-10.5%, enhancing spleen and thymus indices by 0.4-50.1%, or increasing splenocyte lymphocyte proliferation and NK cell activity by 3.4-18.9%.
33618089	0	4	from	impact	4:9	arg1	compositions					82:93	amino acid compositions	71:93	amino acid compositions	71:93	The impact of caseinate oligochitosan-glycation by transglutaminase on amino acid compositions and immune-promoting activity in BALB/c mice of the tryptic caseinate hydrolysate.
33618089	0	4	from	impact	4:9	arg1	activity					116:123	immune-promoting activity	99:123	immune-promoting activity	99:123	The impact of caseinate oligochitosan-glycation by transglutaminase on amino acid compositions and immune-promoting activity in BALB/c mice of the tryptic caseinate hydrolysate.
33618089	5	5	theme	immune-promoting	1219:1234	arg1	activity					1236:1243	improved immune-promoting activity	1210:1243	improved immune-promoting activity once hydrolyzed by trypsin	1210:1270	The results highlight that this TGase-type oligochitosan-glycation is potential to generate functional protein ingredients that possess improved immune-promoting activity once hydrolyzed by trypsin.
33618089	1	6	theme	in	363:364	arg1	activity					388:395	in vivo immune-promoting activity	363:395	in vivo immune-promoting activity	363:395	Caseinate was glycated with oligochitosan via transglutaminase (TGase) action and then hydrolyzed by trypsin to generate glycated caseinate hydrolysate (GCNH) that was investigated for in vivo immune-promoting activity.
33618089	2	7	contain	had	461:463	arg1	CNH					421:423	CNH	421:423	CNH	421:423	Caseinate hydrolysate (CNH) containing glucosamine of 5.7 g/kg had amino acid compositions similar to GCNH.
33618089	2	7	contain	had	461:463	arg1	hydrolysate					408:418	Caseinate hydrolysate	398:418	Caseinate hydrolysate (CNH) containing glucosamine of 5.7 g/kg	398:459	Caseinate hydrolysate (CNH) containing glucosamine of 5.7 g/kg had amino acid compositions similar to GCNH.
33618089	2	7	contain	had	461:463	arg2	compositions					476:487	amino acid compositions	465:487	amino acid compositions similar to GCNH	465:503	Caseinate hydrolysate (CNH) containing glucosamine of 5.7 g/kg had amino acid compositions similar to GCNH.
33618089	3	8	theme	cell	781:784	arg1	activity					786:793	natural killer (NK) cell activity	761:793	natural killer (NK) cell activity	761:793	In normal BALB/c mice, GCNH at 100-400 mg/(kg d) showed higher immune-promoting activity than CNH via increasing serum IgM, IgA, and IgG by 1.5-24.5%, enhancing spleen and thymus indices by 9.7-26.2%, or increasing splenocyte lymphocyte proliferation and natural killer (NK) cell activity by 1.2-11.5%.
33618089	0	9	theme	immune-promoting	99:114	arg1	activity					116:123	immune-promoting activity	99:123	immune-promoting activity	99:123	The impact of caseinate oligochitosan-glycation by transglutaminase on amino acid compositions and immune-promoting activity in BALB/c mice of the tryptic caseinate hydrolysate.
33618089	0	10	theme	BALB/c	128:133	arg1	mice					135:138	BALB/c mice	128:138	BALB/c mice of the tryptic caseinate hydrolysate	128:175	The impact of caseinate oligochitosan-glycation by transglutaminase on amino acid compositions and immune-promoting activity in BALB/c mice of the tryptic caseinate hydrolysate.
33618089	1	11	theme	immune-promoting	371:386	arg1	activity					388:395	in vivo immune-promoting activity	363:395	in vivo immune-promoting activity	363:395	Caseinate was glycated with oligochitosan via transglutaminase (TGase) action and then hydrolyzed by trypsin to generate glycated caseinate hydrolysate (GCNH) that was investigated for in vivo immune-promoting activity.
33618089	4	12	theme	lymphocyte	1014:1023	arg1	proliferation					1025:1037	splenocyte lymphocyte proliferation	1003:1037	splenocyte lymphocyte proliferation	1003:1037	GCNH also exerted higher activity than CNH in the suppressed BALB/c mice through increasing serum IgM, IgA, and IgG by 2.6-10.5%, enhancing spleen and thymus indices by 0.4-50.1%, or increasing splenocyte lymphocyte proliferation and NK cell activity by 3.4-18.9%.
33618089	3	13	theme	normal	509:514	arg1	mice					523:526	normal BALB/c mice	509:526	normal BALB/c mice	509:526	In normal BALB/c mice, GCNH at 100-400 mg/(kg d) showed higher immune-promoting activity than CNH via increasing serum IgM, IgA, and IgG by 1.5-24.5%, enhancing spleen and thymus indices by 9.7-26.2%, or increasing splenocyte lymphocyte proliferation and natural killer (NK) cell activity by 1.2-11.5%.
33618089	2	14	theme	acid	471:474	arg1	compositions					476:487	amino acid compositions	465:487	amino acid compositions similar to GCNH	465:503	Caseinate hydrolysate (CNH) containing glucosamine of 5.7 g/kg had amino acid compositions similar to GCNH.
33618089	5	15	theme	TGase-type	1106:1115	arg1	oligochitosan-glycation					1117:1139	this TGase-type oligochitosan-glycation	1101:1139	this TGase-type oligochitosan-glycation	1101:1139	The results highlight that this TGase-type oligochitosan-glycation is potential to generate functional protein ingredients that possess improved immune-promoting activity once hydrolyzed by trypsin.
33618089	3	16	theme	higher	562:567	arg1	activity					586:593	higher immune-promoting activity	562:593	higher immune-promoting activity	562:593	In normal BALB/c mice, GCNH at 100-400 mg/(kg d) showed higher immune-promoting activity than CNH via increasing serum IgM, IgA, and IgG by 1.5-24.5%, enhancing spleen and thymus indices by 9.7-26.2%, or increasing splenocyte lymphocyte proliferation and natural killer (NK) cell activity by 1.2-11.5%.
33618089	2	17	theme	amino	465:469	arg1	compositions					476:487	amino acid compositions	465:487	amino acid compositions similar to GCNH	465:503	Caseinate hydrolysate (CNH) containing glucosamine of 5.7 g/kg had amino acid compositions similar to GCNH.
33618089	3	18	theme	immune-promoting	569:584	arg1	activity					586:593	higher immune-promoting activity	562:593	higher immune-promoting activity	562:593	In normal BALB/c mice, GCNH at 100-400 mg/(kg d) showed higher immune-promoting activity than CNH via increasing serum IgM, IgA, and IgG by 1.5-24.5%, enhancing spleen and thymus indices by 9.7-26.2%, or increasing splenocyte lymphocyte proliferation and natural killer (NK) cell activity by 1.2-11.5%.
33618089	2	19	theme	Caseinate	398:406	arg1	CNH					421:423	CNH	421:423	CNH	421:423	Caseinate hydrolysate (CNH) containing glucosamine of 5.7 g/kg had amino acid compositions similar to GCNH.
33618089	2	19	theme	Caseinate	398:406	arg1	hydrolysate					408:418	Caseinate hydrolysate	398:418	Caseinate hydrolysate (CNH) containing glucosamine of 5.7 g/kg	398:459	Caseinate hydrolysate (CNH) containing glucosamine of 5.7 g/kg had amino acid compositions similar to GCNH.
33618089	0	20	theme	caseinate	14:22	arg1	oligochitosan-glycation					24:46	caseinate oligochitosan-glycation	14:46	caseinate oligochitosan-glycation by transglutaminase	14:66	The impact of caseinate oligochitosan-glycation by transglutaminase on amino acid compositions and immune-promoting activity in BALB/c mice of the tryptic caseinate hydrolysate.
33618089	1	21	theme	glycated	299:306	arg1	GCNH					331:334	GCNH	331:334	GCNH	331:334	Caseinate was glycated with oligochitosan via transglutaminase (TGase) action and then hydrolyzed by trypsin to generate glycated caseinate hydrolysate (GCNH) that was investigated for in vivo immune-promoting activity.
33618089	1	21	theme	glycated	299:306	arg1	hydrolysate					318:328	glycated caseinate hydrolysate	299:328	glycated caseinate hydrolysate (GCNH) that was investigated for in vivo immune-promoting activity	299:395	Caseinate was glycated with oligochitosan via transglutaminase (TGase) action and then hydrolyzed by trypsin to generate glycated caseinate hydrolysate (GCNH) that was investigated for in vivo immune-promoting activity.
33618089	2	22	theme	5.7 g/kg	452:459	arg1	glucosamine					437:447	glucosamine	437:447	glucosamine of 5.7 g/kg	437:459	Caseinate hydrolysate (CNH) containing glucosamine of 5.7 g/kg had amino acid compositions similar to GCNH.
33618089	1	23	theme	caseinate	308:316	arg1	GCNH					331:334	GCNH	331:334	GCNH	331:334	Caseinate was glycated with oligochitosan via transglutaminase (TGase) action and then hydrolyzed by trypsin to generate glycated caseinate hydrolysate (GCNH) that was investigated for in vivo immune-promoting activity.
33618089	1	23	theme	caseinate	308:316	arg1	hydrolysate					318:328	glycated caseinate hydrolysate	299:328	glycated caseinate hydrolysate (GCNH) that was investigated for in vivo immune-promoting activity	299:395	Caseinate was glycated with oligochitosan via transglutaminase (TGase) action and then hydrolyzed by trypsin to generate glycated caseinate hydrolysate (GCNH) that was investigated for in vivo immune-promoting activity.
33618089	4	24	theme	BALB/c	870:875	arg1	mice					877:880	the suppressed BALB/c mice	855:880	the suppressed BALB/c mice	855:880	GCNH also exerted higher activity than CNH in the suppressed BALB/c mice through increasing serum IgM, IgA, and IgG by 2.6-10.5%, enhancing spleen and thymus indices by 0.4-50.1%, or increasing splenocyte lymphocyte proliferation and NK cell activity by 3.4-18.9%.
33618089	4	25	theme	cell	1046:1049	arg1	activity					1051:1058	NK cell activity	1043:1058	NK cell activity	1043:1058	GCNH also exerted higher activity than CNH in the suppressed BALB/c mice through increasing serum IgM, IgA, and IgG by 2.6-10.5%, enhancing spleen and thymus indices by 0.4-50.1%, or increasing splenocyte lymphocyte proliferation and NK cell activity by 3.4-18.9%.
33618089	1	26	theme	transglutaminase	224:239	arg1	action					249:254	transglutaminase (TGase) action	224:254	transglutaminase (TGase) action	224:254	Caseinate was glycated with oligochitosan via transglutaminase (TGase) action and then hydrolyzed by trypsin to generate glycated caseinate hydrolysate (GCNH) that was investigated for in vivo immune-promoting activity.
33618089	0	27	theme	tryptic	147:153	arg1	hydrolysate					165:175	the tryptic caseinate hydrolysate	143:175	the tryptic caseinate hydrolysate	143:175	The impact of caseinate oligochitosan-glycation by transglutaminase on amino acid compositions and immune-promoting activity in BALB/c mice of the tryptic caseinate hydrolysate.
33618089	0	28	from	compositions	82:93	arg1	mice					135:138	BALB/c mice	128:138	BALB/c mice of the tryptic caseinate hydrolysate	128:175	The impact of caseinate oligochitosan-glycation by transglutaminase on amino acid compositions and immune-promoting activity in BALB/c mice of the tryptic caseinate hydrolysate.
33618089	4	29	theme	NK	1043:1044	arg1	activity					1051:1058	NK cell activity	1043:1058	NK cell activity	1043:1058	GCNH also exerted higher activity than CNH in the suppressed BALB/c mice through increasing serum IgM, IgA, and IgG by 2.6-10.5%, enhancing spleen and thymus indices by 0.4-50.1%, or increasing splenocyte lymphocyte proliferation and NK cell activity by 3.4-18.9%.
33618089	0	30	theme	oligochitosan-glycation	24:46	arg1	impact					4:9	The impact	0:9	The impact of caseinate oligochitosan-glycation by transglutaminase on amino acid compositions and immune-promoting activity in BALB/c mice of the tryptic caseinate hydrolysate.	0:176	The impact of caseinate oligochitosan-glycation by transglutaminase on amino acid compositions and immune-promoting activity in BALB/c mice of the tryptic caseinate hydrolysate.
33618089	2	31	theme	similar	489:495	arg1	compositions					476:487	amino acid compositions	465:487	amino acid compositions similar to GCNH	465:503	Caseinate hydrolysate (CNH) containing glucosamine of 5.7 g/kg had amino acid compositions similar to GCNH.
33618089	3	32	theme	natural	761:767	arg1	NK					777:778	NK	777:778	NK	777:778	In normal BALB/c mice, GCNH at 100-400 mg/(kg d) showed higher immune-promoting activity than CNH via increasing serum IgM, IgA, and IgG by 1.5-24.5%, enhancing spleen and thymus indices by 9.7-26.2%, or increasing splenocyte lymphocyte proliferation and natural killer (NK) cell activity by 1.2-11.5%.
33618089	3	32	theme	natural	761:767	arg1	killer					769:774	natural killer	761:774	natural killer (NK) cell activity	761:793	In normal BALB/c mice, GCNH at 100-400 mg/(kg d) showed higher immune-promoting activity than CNH via increasing serum IgM, IgA, and IgG by 1.5-24.5%, enhancing spleen and thymus indices by 9.7-26.2%, or increasing splenocyte lymphocyte proliferation and natural killer (NK) cell activity by 1.2-11.5%.
33618089	0	33	theme	hydrolysate	165:175	arg1	mice					135:138	BALB/c mice	128:138	BALB/c mice of the tryptic caseinate hydrolysate	128:175	The impact of caseinate oligochitosan-glycation by transglutaminase on amino acid compositions and immune-promoting activity in BALB/c mice of the tryptic caseinate hydrolysate.
33618089	1	34	dep	in	363:364	arg1	vivo					366:369	vivo	366:369	vivo	366:369	Caseinate was glycated with oligochitosan via transglutaminase (TGase) action and then hydrolyzed by trypsin to generate glycated caseinate hydrolysate (GCNH) that was investigated for in vivo immune-promoting activity.
33618089	3	35	theme	splenocyte	721:730	arg1	proliferation					743:755	splenocyte lymphocyte proliferation	721:755	splenocyte lymphocyte proliferation	721:755	In normal BALB/c mice, GCNH at 100-400 mg/(kg d) showed higher immune-promoting activity than CNH via increasing serum IgM, IgA, and IgG by 1.5-24.5%, enhancing spleen and thymus indices by 9.7-26.2%, or increasing splenocyte lymphocyte proliferation and natural killer (NK) cell activity by 1.2-11.5%.
33618089	3	36	theme	lymphocyte	732:741	arg1	proliferation					743:755	splenocyte lymphocyte proliferation	721:755	splenocyte lymphocyte proliferation	721:755	In normal BALB/c mice, GCNH at 100-400 mg/(kg d) showed higher immune-promoting activity than CNH via increasing serum IgM, IgA, and IgG by 1.5-24.5%, enhancing spleen and thymus indices by 9.7-26.2%, or increasing splenocyte lymphocyte proliferation and natural killer (NK) cell activity by 1.2-11.5%.
33618089	0	37	theme	caseinate	155:163	arg1	hydrolysate					165:175	the tryptic caseinate hydrolysate	143:175	the tryptic caseinate hydrolysate	143:175	The impact of caseinate oligochitosan-glycation by transglutaminase on amino acid compositions and immune-promoting activity in BALB/c mice of the tryptic caseinate hydrolysate.
33618089	3	38	theme	BALB/c	516:521	arg1	mice					523:526	normal BALB/c mice	509:526	normal BALB/c mice	509:526	In normal BALB/c mice, GCNH at 100-400 mg/(kg d) showed higher immune-promoting activity than CNH via increasing serum IgM, IgA, and IgG by 1.5-24.5%, enhancing spleen and thymus indices by 9.7-26.2%, or increasing splenocyte lymphocyte proliferation and natural killer (NK) cell activity by 1.2-11.5%.
33618089	5	39	contain	possess	1202:1208	arg2	activity					1236:1243	improved immune-promoting activity	1210:1243	improved immune-promoting activity once hydrolyzed by trypsin	1210:1270	The results highlight that this TGase-type oligochitosan-glycation is potential to generate functional protein ingredients that possess improved immune-promoting activity once hydrolyzed by trypsin.
33618089	5	39	contain	possess	1202:1208	arg1	ingredients					1185:1195	functional protein ingredients	1166:1195	functional protein ingredients that possess improved immune-promoting activity once hydrolyzed by trypsin	1166:1270	The results highlight that this TGase-type oligochitosan-glycation is potential to generate functional protein ingredients that possess improved immune-promoting activity once hydrolyzed by trypsin.
33618089	4	40	theme	suppressed	859:868	arg1	mice					877:880	the suppressed BALB/c mice	855:880	the suppressed BALB/c mice	855:880	GCNH also exerted higher activity than CNH in the suppressed BALB/c mice through increasing serum IgM, IgA, and IgG by 2.6-10.5%, enhancing spleen and thymus indices by 0.4-50.1%, or increasing splenocyte lymphocyte proliferation and NK cell activity by 3.4-18.9%.
33618089	2	41	contain	containing	426:435	arg2	glucosamine					437:447	glucosamine	437:447	glucosamine of 5.7 g/kg	437:459	Caseinate hydrolysate (CNH) containing glucosamine of 5.7 g/kg had amino acid compositions similar to GCNH.
33618089	2	41	contain	containing	426:435	arg1	CNH					421:423	CNH	421:423	CNH	421:423	Caseinate hydrolysate (CNH) containing glucosamine of 5.7 g/kg had amino acid compositions similar to GCNH.
33618089	2	41	contain	containing	426:435	arg1	hydrolysate					408:418	Caseinate hydrolysate	398:418	Caseinate hydrolysate (CNH) containing glucosamine of 5.7 g/kg	398:459	Caseinate hydrolysate (CNH) containing glucosamine of 5.7 g/kg had amino acid compositions similar to GCNH.
33618089	5	42	theme	functional	1166:1175	arg1	ingredients					1185:1195	functional protein ingredients	1166:1195	functional protein ingredients that possess improved immune-promoting activity once hydrolyzed by trypsin	1166:1270	The results highlight that this TGase-type oligochitosan-glycation is potential to generate functional protein ingredients that possess improved immune-promoting activity once hydrolyzed by trypsin.
33618089	0	43	from	activity	116:123	arg1	mice					135:138	BALB/c mice	128:138	BALB/c mice of the tryptic caseinate hydrolysate	128:175	The impact of caseinate oligochitosan-glycation by transglutaminase on amino acid compositions and immune-promoting activity in BALB/c mice of the tryptic caseinate hydrolysate.
33618089	4	44	theme	thymus	960:965	arg1	indices					967:973	thymus indices	960:973	thymus indices	960:973	GCNH also exerted higher activity than CNH in the suppressed BALB/c mice through increasing serum IgM, IgA, and IgG by 2.6-10.5%, enhancing spleen and thymus indices by 0.4-50.1%, or increasing splenocyte lymphocyte proliferation and NK cell activity by 3.4-18.9%.
33618089	5	45	theme	protein	1177:1183	arg1	ingredients					1185:1195	functional protein ingredients	1166:1195	functional protein ingredients that possess improved immune-promoting activity once hydrolyzed by trypsin	1166:1270	The results highlight that this TGase-type oligochitosan-glycation is potential to generate functional protein ingredients that possess improved immune-promoting activity once hydrolyzed by trypsin.
33618089	0	46	theme	acid	77:80	arg1	compositions					82:93	amino acid compositions	71:93	amino acid compositions	71:93	The impact of caseinate oligochitosan-glycation by transglutaminase on amino acid compositions and immune-promoting activity in BALB/c mice of the tryptic caseinate hydrolysate.
33618089	4	47	theme	splenocyte	1003:1012	arg1	proliferation					1025:1037	splenocyte lymphocyte proliferation	1003:1037	splenocyte lymphocyte proliferation	1003:1037	GCNH also exerted higher activity than CNH in the suppressed BALB/c mice through increasing serum IgM, IgA, and IgG by 2.6-10.5%, enhancing spleen and thymus indices by 0.4-50.1%, or increasing splenocyte lymphocyte proliferation and NK cell activity by 3.4-18.9%.
33618089	3	48	theme	killer	769:774	arg1	activity					786:793	natural killer (NK) cell activity	761:793	natural killer (NK) cell activity	761:793	In normal BALB/c mice, GCNH at 100-400 mg/(kg d) showed higher immune-promoting activity than CNH via increasing serum IgM, IgA, and IgG by 1.5-24.5%, enhancing spleen and thymus indices by 9.7-26.2%, or increasing splenocyte lymphocyte proliferation and natural killer (NK) cell activity by 1.2-11.5%.
33618089	0	49	theme	amino	71:75	arg1	compositions					82:93	amino acid compositions	71:93	amino acid compositions	71:93	The impact of caseinate oligochitosan-glycation by transglutaminase on amino acid compositions and immune-promoting activity in BALB/c mice of the tryptic caseinate hydrolysate.
33618089	3	50	theme	serum	619:623	arg1	IgM					625:627	serum IgM	619:627	serum IgM	619:627	In normal BALB/c mice, GCNH at 100-400 mg/(kg d) showed higher immune-promoting activity than CNH via increasing serum IgM, IgA, and IgG by 1.5-24.5%, enhancing spleen and thymus indices by 9.7-26.2%, or increasing splenocyte lymphocyte proliferation and natural killer (NK) cell activity by 1.2-11.5%.
33618089	3	51	from	100-400 mg/	537:547	arg1	GCNH					529:532	GCNH	529:532	GCNH at 100-400 mg/(kg d)	529:553	In normal BALB/c mice, GCNH at 100-400 mg/(kg d) showed higher immune-promoting activity than CNH via increasing serum IgM, IgA, and IgG by 1.5-24.5%, enhancing spleen and thymus indices by 9.7-26.2%, or increasing splenocyte lymphocyte proliferation and natural killer (NK) cell activity by 1.2-11.5%.
33618089	4	52	theme	serum	901:905	arg1	IgM					907:909	serum IgM	901:909	serum IgM	901:909	GCNH also exerted higher activity than CNH in the suppressed BALB/c mice through increasing serum IgM, IgA, and IgG by 2.6-10.5%, enhancing spleen and thymus indices by 0.4-50.1%, or increasing splenocyte lymphocyte proliferation and NK cell activity by 3.4-18.9%.
34627996	7	0	theme	biological	1217:1226	arg1	media					1228:1232	biological media	1217:1232	biological media	1217:1232	This investigation on morphology-dependent self-motion of nanomaterials could have a significant impact on drug delivery applications by increasing particle transport through biological media.
34627996	1	1	theme	particle	165:172	arg1	transport					174:182	inefficient particle transport	153:182	inefficient particle transport through biological media	153:207	Drug delivery to target sites is often limited by inefficient particle transport through biological media.
34627996	7	2	from	impact	1139:1144	arg1	applications					1163:1174	drug delivery applications	1149:1174	drug delivery applications	1149:1174	This investigation on morphology-dependent self-motion of nanomaterials could have a significant impact on drug delivery applications by increasing particle transport through biological media.
34627996	7	3	theme	delivery	1154:1161	arg1	applications					1163:1174	drug delivery applications	1149:1174	drug delivery applications	1149:1174	This investigation on morphology-dependent self-motion of nanomaterials could have a significant impact on drug delivery applications by increasing particle transport through biological media.
34627996	5	4	theme	scale	793:797	arg1	plots					750:754	the plots	746:754	the plots of mean square displacement vs. time scale	746:797	Fitting the plots of mean square displacement vs. time scale suggests that nanoplatelets exhibited hyperdiffusive behavior, which is similar to the motion of living microorganisms.
34627996	1	5	theme	Drug	103:106	arg1	delivery					108:115	Drug delivery	103:115	Drug delivery to target sites	103:131	Drug delivery to target sites is often limited by inefficient particle transport through biological media.
34627996	4	6	with	ratio	622:626	arg1	morphology					593:602	flattened morphology	583:602	flattened morphology	583:602	The analysis of nanomaterial trajectories revealed that particles with flattened morphology and a high aspect ratio, designated nanoplatelets, exhibited more linear trajectories and faster diffusion in water than nanospheres.
34627996	7	7	contain	have	1120:1123	arg1	investigation					1047:1059	This investigation	1042:1059	This investigation on morphology-dependent self-motion of nanomaterials	1042:1112	This investigation on morphology-dependent self-motion of nanomaterials could have a significant impact on drug delivery applications by increasing particle transport through biological media.
34627996	7	7	contain	have	1120:1123	arg2	impact					1139:1144	a significant impact	1125:1144	a significant impact on drug delivery applications	1125:1174	This investigation on morphology-dependent self-motion of nanomaterials could have a significant impact on drug delivery applications by increasing particle transport through biological media.
34627996	4	8	theme	trajectories	541:552	arg1	analysis					516:523	The analysis	512:523	The analysis of nanomaterial trajectories	512:552	The analysis of nanomaterial trajectories revealed that particles with flattened morphology and a high aspect ratio, designated nanoplatelets, exhibited more linear trajectories and faster diffusion in water than nanospheres.
34627996	7	9	theme	particle	1190:1197	arg1	transport					1199:1207	particle transport	1190:1207	particle transport	1190:1207	This investigation on morphology-dependent self-motion of nanomaterials could have a significant impact on drug delivery applications by increasing particle transport through biological media.
34627996	3	10	contain	have	415:418	arg2	potentials					464:473	surface potentials	456:473	surface potentials	456:473	The two types of nanomaterials have comparable surface compositions and surface potentials, and they have equivalent diameters.
34627996	3	10	contain	have	415:418	arg1	types					392:396	The two types	384:396	The two types of nanomaterials	384:413	The two types of nanomaterials have comparable surface compositions and surface potentials, and they have equivalent diameters.
34627996	3	10	contain	have	415:418	arg2	compositions					439:450	comparable surface compositions	420:450	comparable surface compositions	420:450	The two types of nanomaterials have comparable surface compositions and surface potentials, and they have equivalent diameters.
34627996	0	11	theme	Real-time	0:8	arg1	visualization					10:22	Real-time visualization	0:22	Real-time visualization of morphology-dependent self-motion of hyaluronic acid	0:77	Real-time visualization of morphology-dependent self-motion of hyaluronic acid nanomaterials in water.
34627996	1	12	theme	biological	192:201	arg1	media					203:207	biological media	192:207	biological media	192:207	Drug delivery to target sites is often limited by inefficient particle transport through biological media.
34627996	3	13	theme	comparable	420:429	arg1	compositions					439:450	comparable surface compositions	420:450	comparable surface compositions	420:450	The two types of nanomaterials have comparable surface compositions and surface potentials, and they have equivalent diameters.
34627996	4	14	theme	nanomaterial	528:539	arg1	trajectories					541:552	nanomaterial trajectories	528:552	nanomaterial trajectories	528:552	The analysis of nanomaterial trajectories revealed that particles with flattened morphology and a high aspect ratio, designated nanoplatelets, exhibited more linear trajectories and faster diffusion in water than nanospheres.
34627996	2	15	theme	multiple	345:352	arg1	technology					372:381	real-time multiple particle tracking technology	335:381	real-time multiple particle tracking technology	335:381	Herein, motion behaviors of spherical and nonspherical nanomaterials composed of hyaluronic acid were studied in water using real-time multiple particle tracking technology.
34627996	7	16	theme	significant	1127:1137	arg1	impact					1139:1144	a significant impact	1125:1144	a significant impact on drug delivery applications	1125:1174	This investigation on morphology-dependent self-motion of nanomaterials could have a significant impact on drug delivery applications by increasing particle transport through biological media.
34627996	7	17	from	investigation	1047:1059	arg1	self-motion					1085:1095	morphology-dependent self-motion	1064:1095	morphology-dependent self-motion of nanomaterials	1064:1112	This investigation on morphology-dependent self-motion of nanomaterials could have a significant impact on drug delivery applications by increasing particle transport through biological media.
34627996	3	18	theme	surface	431:437	arg1	compositions					439:450	comparable surface compositions	420:450	comparable surface compositions	420:450	The two types of nanomaterials have comparable surface compositions and surface potentials, and they have equivalent diameters.
34627996	4	19	theme	aspect	615:620	arg1	ratio					622:626	a high aspect ratio	608:626	a high aspect ratio	608:626	The analysis of nanomaterial trajectories revealed that particles with flattened morphology and a high aspect ratio, designated nanoplatelets, exhibited more linear trajectories and faster diffusion in water than nanospheres.
34627996	2	20	theme	real-time	335:343	arg1	technology					372:381	real-time multiple particle tracking technology	335:381	real-time multiple particle tracking technology	335:381	Herein, motion behaviors of spherical and nonspherical nanomaterials composed of hyaluronic acid were studied in water using real-time multiple particle tracking technology.
34627996	0	21	theme	morphology-dependent	27:46	arg1	self-motion					48:58	morphology-dependent self-motion	27:58	morphology-dependent self-motion of hyaluronic acid	27:77	Real-time visualization of morphology-dependent self-motion of hyaluronic acid nanomaterials in water.
34627996	4	22	theme	high	610:613	arg1	ratio					622:626	a high aspect ratio	608:626	a high aspect ratio	608:626	The analysis of nanomaterial trajectories revealed that particles with flattened morphology and a high aspect ratio, designated nanoplatelets, exhibited more linear trajectories and faster diffusion in water than nanospheres.
34627996	5	23	theme	square	764:769	arg1	displacement					771:782	mean square displacement	759:782	mean square displacement	759:782	Fitting the plots of mean square displacement vs. time scale suggests that nanoplatelets exhibited hyperdiffusive behavior, which is similar to the motion of living microorganisms.
34627996	5	24	theme	microorganisms	903:916	arg1	motion					886:891	the motion	882:891	the motion of living microorganisms	882:916	Fitting the plots of mean square displacement vs. time scale suggests that nanoplatelets exhibited hyperdiffusive behavior, which is similar to the motion of living microorganisms.
34627996	5	25	theme	living	896:901	arg1	microorganisms					903:916	living microorganisms	896:916	living microorganisms	896:916	Fitting the plots of mean square displacement vs. time scale suggests that nanoplatelets exhibited hyperdiffusive behavior, which is similar to the motion of living microorganisms.
34627996	5	26	theme	displacement	771:782	arg1	plots					750:754	the plots	746:754	the plots of mean square displacement vs. time scale	746:797	Fitting the plots of mean square displacement vs. time scale suggests that nanoplatelets exhibited hyperdiffusive behavior, which is similar to the motion of living microorganisms.
34627996	1	27	theme	target	120:125	arg1	sites					127:131	target sites	120:131	target sites	120:131	Drug delivery to target sites is often limited by inefficient particle transport through biological media.
34627996	4	28	from	diffusion	701:709	arg1	water					714:718	water	714:718	water	714:718	The analysis of nanomaterial trajectories revealed that particles with flattened morphology and a high aspect ratio, designated nanoplatelets, exhibited more linear trajectories and faster diffusion in water than nanospheres.
34627996	4	29	theme	faster	694:699	arg1	diffusion					701:709	faster diffusion	694:709	faster diffusion in water	694:718	The analysis of nanomaterial trajectories revealed that particles with flattened morphology and a high aspect ratio, designated nanoplatelets, exhibited more linear trajectories and faster diffusion in water than nanospheres.
34627996	3	30	theme	surface	456:462	arg1	potentials					464:473	surface potentials	456:473	surface potentials	456:473	The two types of nanomaterials have comparable surface compositions and surface potentials, and they have equivalent diameters.
34627996	6	31	dep	33-fold	991:997	arg1	higher					999:1004	higher	999:1004	higher	999:1004	Furthermore, at 37 °C, the surface explored by a nanoplatelet was up to 33-fold higher than that explored by a nanosphere.
34627996	6	31	dep	33-fold	991:997	arg1	to					988:989	to	988:989	to	988:989	Furthermore, at 37 °C, the surface explored by a nanoplatelet was up to 33-fold higher than that explored by a nanosphere.
34627996	2	32	theme	nonspherical	252:263	arg1	nanomaterials					265:277	spherical and nonspherical nanomaterials	238:277	spherical and nonspherical nanomaterials composed of hyaluronic acid	238:305	Herein, motion behaviors of spherical and nonspherical nanomaterials composed of hyaluronic acid were studied in water using real-time multiple particle tracking technology.
34627996	3	33	theme	equivalent	490:499	arg1	diameters					501:509	equivalent diameters	490:509	equivalent diameters	490:509	The two types of nanomaterials have comparable surface compositions and surface potentials, and they have equivalent diameters.
34627996	0	34	theme	self-motion	48:58	arg1	visualization					10:22	Real-time visualization	0:22	Real-time visualization of morphology-dependent self-motion of hyaluronic acid	0:77	Real-time visualization of morphology-dependent self-motion of hyaluronic acid nanomaterials in water.
34627996	5	35	theme	time	788:791	arg1	scale					793:797	time scale	788:797	time scale	788:797	Fitting the plots of mean square displacement vs. time scale suggests that nanoplatelets exhibited hyperdiffusive behavior, which is similar to the motion of living microorganisms.
34627996	3	36	theme	nanomaterials	401:413	arg1	types					392:396	The two types	384:396	The two types of nanomaterials	384:413	The two types of nanomaterials have comparable surface compositions and surface potentials, and they have equivalent diameters.
34627996	0	37	theme	acid	74:77	arg1	self-motion					48:58	morphology-dependent self-motion	27:58	morphology-dependent self-motion of hyaluronic acid	27:77	Real-time visualization of morphology-dependent self-motion of hyaluronic acid nanomaterials in water.
34627996	4	38	theme	flattened	583:591	arg1	morphology					593:602	flattened morphology	583:602	flattened morphology	583:602	The analysis of nanomaterial trajectories revealed that particles with flattened morphology and a high aspect ratio, designated nanoplatelets, exhibited more linear trajectories and faster diffusion in water than nanospheres.
34627996	5	39	theme	hyperdiffusive	837:850	arg1	behavior					852:859	hyperdiffusive behavior	837:859	hyperdiffusive behavior	837:859	Fitting the plots of mean square displacement vs. time scale suggests that nanoplatelets exhibited hyperdiffusive behavior, which is similar to the motion of living microorganisms.
34627996	2	40	theme	spherical	238:246	arg1	nanomaterials					265:277	spherical and nonspherical nanomaterials	238:277	spherical and nonspherical nanomaterials composed of hyaluronic acid	238:305	Herein, motion behaviors of spherical and nonspherical nanomaterials composed of hyaluronic acid were studied in water using real-time multiple particle tracking technology.
34627996	7	41	theme	nanomaterials	1100:1112	arg1	self-motion					1085:1095	morphology-dependent self-motion	1064:1095	morphology-dependent self-motion of nanomaterials	1064:1112	This investigation on morphology-dependent self-motion of nanomaterials could have a significant impact on drug delivery applications by increasing particle transport through biological media.
34627996	0	42	theme	hyaluronic	63:72	arg1	acid					74:77	hyaluronic acid	63:77	hyaluronic acid	63:77	Real-time visualization of morphology-dependent self-motion of hyaluronic acid nanomaterials in water.
34627996	7	43	theme	drug	1149:1152	arg1	applications					1163:1174	drug delivery applications	1149:1174	drug delivery applications	1149:1174	This investigation on morphology-dependent self-motion of nanomaterials could have a significant impact on drug delivery applications by increasing particle transport through biological media.
34627996	7	44	theme	morphology-dependent	1064:1083	arg1	self-motion					1085:1095	morphology-dependent self-motion	1064:1095	morphology-dependent self-motion of nanomaterials	1064:1112	This investigation on morphology-dependent self-motion of nanomaterials could have a significant impact on drug delivery applications by increasing particle transport through biological media.
34627996	4	45	with	particles	568:576	arg1	morphology					593:602	flattened morphology	583:602	flattened morphology	583:602	The analysis of nanomaterial trajectories revealed that particles with flattened morphology and a high aspect ratio, designated nanoplatelets, exhibited more linear trajectories and faster diffusion in water than nanospheres.
34627996	2	46	theme	motion	218:223	arg1	behaviors					225:233	motion behaviors	218:233	motion behaviors of spherical and nonspherical nanomaterials composed of hyaluronic acid	218:305	Herein, motion behaviors of spherical and nonspherical nanomaterials composed of hyaluronic acid were studied in water using real-time multiple particle tracking technology.
34627996	2	47	theme	hyaluronic	291:300	arg1	acid					302:305	hyaluronic acid	291:305	hyaluronic acid	291:305	Herein, motion behaviors of spherical and nonspherical nanomaterials composed of hyaluronic acid were studied in water using real-time multiple particle tracking technology.
34627996	2	48	theme	tracking	363:370	arg1	technology					372:381	real-time multiple particle tracking technology	335:381	real-time multiple particle tracking technology	335:381	Herein, motion behaviors of spherical and nonspherical nanomaterials composed of hyaluronic acid were studied in water using real-time multiple particle tracking technology.
34627996	4	49	theme	linear	670:675	arg1	trajectories					677:688	trajectories	677:688	trajectories	677:688	The analysis of nanomaterial trajectories revealed that particles with flattened morphology and a high aspect ratio, designated nanoplatelets, exhibited more linear trajectories and faster diffusion in water than nanospheres.
34627996	5	50	theme	mean	759:762	arg1	displacement					771:782	mean square displacement	759:782	mean square displacement	759:782	Fitting the plots of mean square displacement vs. time scale suggests that nanoplatelets exhibited hyperdiffusive behavior, which is similar to the motion of living microorganisms.
34627996	3	51	contain	have	485:488	arg1	they					480:483	they	480:483	they	480:483	The two types of nanomaterials have comparable surface compositions and surface potentials, and they have equivalent diameters.
34627996	3	51	contain	have	485:488	arg2	diameters					501:509	equivalent diameters	490:509	equivalent diameters	490:509	The two types of nanomaterials have comparable surface compositions and surface potentials, and they have equivalent diameters.
34627996	2	52	theme	particle	354:361	arg1	technology					372:381	real-time multiple particle tracking technology	335:381	real-time multiple particle tracking technology	335:381	Herein, motion behaviors of spherical and nonspherical nanomaterials composed of hyaluronic acid were studied in water using real-time multiple particle tracking technology.
34627996	4	53	from	trajectories	677:688	arg1	water					714:718	water	714:718	water	714:718	The analysis of nanomaterial trajectories revealed that particles with flattened morphology and a high aspect ratio, designated nanoplatelets, exhibited more linear trajectories and faster diffusion in water than nanospheres.
34627996	2	54	theme	nanomaterials	265:277	arg1	behaviors					225:233	motion behaviors	218:233	motion behaviors of spherical and nonspherical nanomaterials composed of hyaluronic acid	218:305	Herein, motion behaviors of spherical and nonspherical nanomaterials composed of hyaluronic acid were studied in water using real-time multiple particle tracking technology.
34627996	1	55	theme	inefficient	153:163	arg1	transport					174:182	inefficient particle transport	153:182	inefficient particle transport through biological media	153:207	Drug delivery to target sites is often limited by inefficient particle transport through biological media.
32414428	7	0	theme	tanning	1158:1164	arg1	approach					1166:1173	ecological tanning approach	1147:1173	ecological tanning approach	1147:1173	These findings serve as a foundation for the minimization of formaldehyde in DCMC and the development of ecological tanning approach.
32414428	1	1	theme	tanning	170:176	arg1	agent					178:182	dialdehyde carboxymethyl cellulose (DCMC) tanning agent	128:182	dialdehyde carboxymethyl cellulose (DCMC) tanning agent prepared through periodate oxidation of sodium carboxymethyl cellulose (CMC)	128:259	Formaldehyde was detected in dialdehyde carboxymethyl cellulose (DCMC) tanning agent prepared through periodate oxidation of sodium carboxymethyl cellulose (CMC).
32414428	7	2	from	minimization	1087:1098	arg1	DCMC					1119:1122	DCMC	1119:1122	DCMC	1119:1122	These findings serve as a foundation for the minimization of formaldehyde in DCMC and the development of ecological tanning approach.
32414428	0	3	theme	agent	92:96	arg1	preparation					34:44	the preparation	30:44	the preparation of dialdehyde carboxymethyl cellulose tanning agent	30:96	Formaldehyde formation during the preparation of dialdehyde carboxymethyl cellulose tanning agent.
32414428	7	4	theme	approach	1166:1173	arg1	findings					1048:1055	These findings	1042:1055	These findings	1042:1055	These findings serve as a foundation for the minimization of formaldehyde in DCMC and the development of ecological tanning approach.
32414428	7	4	theme	approach	1166:1173	arg1	development					1132:1142	the development	1128:1142	the development of ecological tanning approach	1128:1173	These findings serve as a foundation for the minimization of formaldehyde in DCMC and the development of ecological tanning approach.
32414428	7	4	theme	approach	1166:1173	arg1	foundation					1068:1077	a foundation	1066:1077	a foundation for the minimization of formaldehyde in DCMC	1066:1122	These findings serve as a foundation for the minimization of formaldehyde in DCMC and the development of ecological tanning approach.
32414428	3	5	theme	sugar	547:551	arg1	structures					558:567	sugar unit structures	547:567	sugar unit structures	547:567	The formation mechanism of formaldehyde in DCMC was investigated by composition analysis and intermediate identification of DCMC with different polymerization degrees and sugar unit structures.
32414428	4	6	theme	C-6	621:623	arg1	overoxidation					604:616	the overoxidation	600:616	the overoxidation of C-6 on the reducing glucose residue of CMC	600:662	Formaldehyde was derived from the overoxidation of C-6 on the reducing glucose residue of CMC.
32414428	6	7	theme	glucose	937:943	arg1	formation					895:903	the formation	891:903	the formation of reducing glucose residue and glucose	891:943	The low degradation degree and high degree of substitution of CMC reduced the possibility of the formation of reducing glucose residue and glucose during oxidation, thereby resulting in low formaldehyde content in DCMC and DCMC-tanned leather.
32414428	6	8	theme	formation	895:903	arg1	possibility					876:886	the possibility	872:886	the possibility of the formation of reducing glucose residue and glucose	872:943	The low degradation degree and high degree of substitution of CMC reduced the possibility of the formation of reducing glucose residue and glucose during oxidation, thereby resulting in low formaldehyde content in DCMC and DCMC-tanned leather.
32414428	2	9	theme	DCMC	316:319	arg1	tanning					321:327	DCMC tanning	316:327	DCMC tanning	316:327	Formaldehyde was then introduced into leather through DCMC tanning, which poses a potential risk to human health.
32414428	4	10	attach	derived	587:593	arg1	overoxidation					604:616	the overoxidation	600:616	the overoxidation of C-6 on the reducing glucose residue of CMC	600:662	Formaldehyde was derived from the overoxidation of C-6 on the reducing glucose residue of CMC.
32414428	4	10	attach	derived	587:593	arg2	Formaldehyde					570:581	Formaldehyde	570:581	Formaldehyde	570:581	Formaldehyde was derived from the overoxidation of C-6 on the reducing glucose residue of CMC.
32414428	4	11	theme	reducing	632:639	arg1	residue					649:655	the reducing glucose residue	628:655	the reducing glucose residue of CMC	628:662	Formaldehyde was derived from the overoxidation of C-6 on the reducing glucose residue of CMC.
32414428	4	11	theme	reducing	632:639	arg1	CMC					660:662	CMC	660:662	CMC	660:662	Formaldehyde was derived from the overoxidation of C-6 on the reducing glucose residue of CMC.
32414428	7	12	theme	ecological	1147:1156	arg1	approach					1166:1173	ecological tanning approach	1147:1173	ecological tanning approach	1147:1173	These findings serve as a foundation for the minimization of formaldehyde in DCMC and the development of ecological tanning approach.
32414428	3	13	theme	polymerization	520:533	arg1	degrees					535:541	different polymerization degrees	510:541	different polymerization degrees	510:541	The formation mechanism of formaldehyde in DCMC was investigated by composition analysis and intermediate identification of DCMC with different polymerization degrees and sugar unit structures.
32414428	3	14	theme	DCMC	500:503	arg1	analysis					456:463	composition analysis	444:463	composition analysis	444:463	The formation mechanism of formaldehyde in DCMC was investigated by composition analysis and intermediate identification of DCMC with different polymerization degrees and sugar unit structures.
32414428	3	14	theme	DCMC	500:503	arg1	identification					482:495	intermediate identification	469:495	intermediate identification	469:495	The formation mechanism of formaldehyde in DCMC was investigated by composition analysis and intermediate identification of DCMC with different polymerization degrees and sugar unit structures.
32414428	6	15	theme	glucose	917:923	arg1	residue					925:931	reducing glucose residue	908:931	reducing glucose residue	908:931	The low degradation degree and high degree of substitution of CMC reduced the possibility of the formation of reducing glucose residue and glucose during oxidation, thereby resulting in low formaldehyde content in DCMC and DCMC-tanned leather.
32414428	6	16	theme	high	829:832	arg1	degree					834:839	high degree	829:839	high degree	829:839	The low degradation degree and high degree of substitution of CMC reduced the possibility of the formation of reducing glucose residue and glucose during oxidation, thereby resulting in low formaldehyde content in DCMC and DCMC-tanned leather.
32414428	4	17	theme	glucose	641:647	arg1	residue					649:655	the reducing glucose residue	628:655	the reducing glucose residue of CMC	628:662	Formaldehyde was derived from the overoxidation of C-6 on the reducing glucose residue of CMC.
32414428	4	17	theme	glucose	641:647	arg1	CMC					660:662	CMC	660:662	CMC	660:662	Formaldehyde was derived from the overoxidation of C-6 on the reducing glucose residue of CMC.
32414428	6	18	theme	reducing	908:915	arg1	residue					925:931	reducing glucose residue	908:931	reducing glucose residue	908:931	The low degradation degree and high degree of substitution of CMC reduced the possibility of the formation of reducing glucose residue and glucose during oxidation, thereby resulting in low formaldehyde content in DCMC and DCMC-tanned leather.
32414428	6	19	theme	residue	925:931	arg1	formation					895:903	the formation	891:903	the formation of reducing glucose residue and glucose	891:943	The low degradation degree and high degree of substitution of CMC reduced the possibility of the formation of reducing glucose residue and glucose during oxidation, thereby resulting in low formaldehyde content in DCMC and DCMC-tanned leather.
32414428	3	20	from	mechanism	390:398	arg1	DCMC					419:422	DCMC	419:422	DCMC	419:422	The formation mechanism of formaldehyde in DCMC was investigated by composition analysis and intermediate identification of DCMC with different polymerization degrees and sugar unit structures.
32414428	1	21	theme	periodate	201:209	arg1	oxidation					211:219	periodate oxidation	201:219	periodate oxidation of sodium carboxymethyl cellulose (CMC)	201:259	Formaldehyde was detected in dialdehyde carboxymethyl cellulose (DCMC) tanning agent prepared through periodate oxidation of sodium carboxymethyl cellulose (CMC).
32414428	3	22	theme	formaldehyde	403:414	arg1	mechanism					390:398	The formation mechanism	376:398	The formation mechanism of formaldehyde in DCMC	376:422	The formation mechanism of formaldehyde in DCMC was investigated by composition analysis and intermediate identification of DCMC with different polymerization degrees and sugar unit structures.
32414428	3	23	theme	composition	444:454	arg1	analysis					456:463	composition analysis	444:463	composition analysis	444:463	The formation mechanism of formaldehyde in DCMC was investigated by composition analysis and intermediate identification of DCMC with different polymerization degrees and sugar unit structures.
32414428	3	24	theme	formation	380:388	arg1	mechanism					390:398	The formation mechanism	376:398	The formation mechanism of formaldehyde in DCMC	376:422	The formation mechanism of formaldehyde in DCMC was investigated by composition analysis and intermediate identification of DCMC with different polymerization degrees and sugar unit structures.
32414428	4	25	from	overoxidation	604:616	arg1	residue					649:655	the reducing glucose residue	628:655	the reducing glucose residue of CMC	628:662	Formaldehyde was derived from the overoxidation of C-6 on the reducing glucose residue of CMC.
32414428	4	25	from	overoxidation	604:616	arg1	CMC					660:662	CMC	660:662	CMC	660:662	Formaldehyde was derived from the overoxidation of C-6 on the reducing glucose residue of CMC.
32414428	6	26	from	content	1001:1007	arg1	leather					1033:1039	DCMC-tanned leather	1021:1039	DCMC-tanned leather	1021:1039	The low degradation degree and high degree of substitution of CMC reduced the possibility of the formation of reducing glucose residue and glucose during oxidation, thereby resulting in low formaldehyde content in DCMC and DCMC-tanned leather.
32414428	6	26	from	content	1001:1007	arg1	DCMC					1012:1015	DCMC	1012:1015	DCMC	1012:1015	The low degradation degree and high degree of substitution of CMC reduced the possibility of the formation of reducing glucose residue and glucose during oxidation, thereby resulting in low formaldehyde content in DCMC and DCMC-tanned leather.
32414428	6	27	theme	degradation	806:816	arg1	degree					818:823	The low degradation degree	798:823	The low degradation degree	798:823	The low degradation degree and high degree of substitution of CMC reduced the possibility of the formation of reducing glucose residue and glucose during oxidation, thereby resulting in low formaldehyde content in DCMC and DCMC-tanned leather.
32414428	2	28	theme	human	362:366	arg1	health					368:373	human health	362:373	human health	362:373	Formaldehyde was then introduced into leather through DCMC tanning, which poses a potential risk to human health.
32414428	1	29	theme	sodium	224:229	arg1	CMC					256:258	CMC	256:258	CMC	256:258	Formaldehyde was detected in dialdehyde carboxymethyl cellulose (DCMC) tanning agent prepared through periodate oxidation of sodium carboxymethyl cellulose (CMC).
32414428	1	29	theme	sodium	224:229	arg1	cellulose					245:253	sodium carboxymethyl cellulose	224:253	sodium carboxymethyl cellulose (CMC)	224:259	Formaldehyde was detected in dialdehyde carboxymethyl cellulose (DCMC) tanning agent prepared through periodate oxidation of sodium carboxymethyl cellulose (CMC).
32414428	6	30	theme	formaldehyde	988:999	arg1	content					1001:1007	low formaldehyde content	984:1007	low formaldehyde content in DCMC and DCMC-tanned leather	984:1039	The low degradation degree and high degree of substitution of CMC reduced the possibility of the formation of reducing glucose residue and glucose during oxidation, thereby resulting in low formaldehyde content in DCMC and DCMC-tanned leather.
32414428	3	31	theme	intermediate	469:480	arg1	identification					482:495	intermediate identification	469:495	intermediate identification	469:495	The formation mechanism of formaldehyde in DCMC was investigated by composition analysis and intermediate identification of DCMC with different polymerization degrees and sugar unit structures.
32414428	1	32	theme	carboxymethyl	231:243	arg1	CMC					256:258	CMC	256:258	CMC	256:258	Formaldehyde was detected in dialdehyde carboxymethyl cellulose (DCMC) tanning agent prepared through periodate oxidation of sodium carboxymethyl cellulose (CMC).
32414428	1	32	theme	carboxymethyl	231:243	arg1	cellulose					245:253	sodium carboxymethyl cellulose	224:253	sodium carboxymethyl cellulose (CMC)	224:259	Formaldehyde was detected in dialdehyde carboxymethyl cellulose (DCMC) tanning agent prepared through periodate oxidation of sodium carboxymethyl cellulose (CMC).
32414428	6	33	theme	low	984:986	arg1	content					1001:1007	low formaldehyde content	984:1007	low formaldehyde content in DCMC and DCMC-tanned leather	984:1039	The low degradation degree and high degree of substitution of CMC reduced the possibility of the formation of reducing glucose residue and glucose during oxidation, thereby resulting in low formaldehyde content in DCMC and DCMC-tanned leather.
32414428	3	34	with	DCMC	500:503	arg1	degrees					535:541	different polymerization degrees	510:541	different polymerization degrees	510:541	The formation mechanism of formaldehyde in DCMC was investigated by composition analysis and intermediate identification of DCMC with different polymerization degrees and sugar unit structures.
32414428	3	34	with	DCMC	500:503	arg1	structures					558:567	sugar unit structures	547:567	sugar unit structures	547:567	The formation mechanism of formaldehyde in DCMC was investigated by composition analysis and intermediate identification of DCMC with different polymerization degrees and sugar unit structures.
32414428	6	35	theme	low	802:804	arg1	degree					818:823	The low degradation degree	798:823	The low degradation degree	798:823	The low degradation degree and high degree of substitution of CMC reduced the possibility of the formation of reducing glucose residue and glucose during oxidation, thereby resulting in low formaldehyde content in DCMC and DCMC-tanned leather.
32414428	1	36	theme	cellulose	245:253	arg1	oxidation					211:219	periodate oxidation	201:219	periodate oxidation of sodium carboxymethyl cellulose (CMC)	201:259	Formaldehyde was detected in dialdehyde carboxymethyl cellulose (DCMC) tanning agent prepared through periodate oxidation of sodium carboxymethyl cellulose (CMC).
32414428	0	37	theme	carboxymethyl	60:72	arg1	agent					92:96	dialdehyde carboxymethyl cellulose tanning agent	49:96	dialdehyde carboxymethyl cellulose tanning agent	49:96	Formaldehyde formation during the preparation of dialdehyde carboxymethyl cellulose tanning agent.
32414428	5	38	theme	concomitant	705:715	arg1	degradation					717:727	the concomitant degradation	701:727	the concomitant degradation of CMC	701:734	Moreover, glucose was produced from the concomitant degradation of CMC during oxidation, and then oxidized to liberate formaldehyde.
32414428	1	39	theme	dialdehyde	128:137	arg1	agent					178:182	dialdehyde carboxymethyl cellulose (DCMC) tanning agent	128:182	dialdehyde carboxymethyl cellulose (DCMC) tanning agent prepared through periodate oxidation of sodium carboxymethyl cellulose (CMC)	128:259	Formaldehyde was detected in dialdehyde carboxymethyl cellulose (DCMC) tanning agent prepared through periodate oxidation of sodium carboxymethyl cellulose (CMC).
32414428	0	40	theme	dialdehyde	49:58	arg1	agent					92:96	dialdehyde carboxymethyl cellulose tanning agent	49:96	dialdehyde carboxymethyl cellulose tanning agent	49:96	Formaldehyde formation during the preparation of dialdehyde carboxymethyl cellulose tanning agent.
32414428	7	41	theme	formaldehyde	1103:1114	arg1	minimization					1087:1098	the minimization	1083:1098	the minimization of formaldehyde in DCMC	1083:1122	These findings serve as a foundation for the minimization of formaldehyde in DCMC and the development of ecological tanning approach.
32414428	1	42	theme	carboxymethyl	139:151	arg1	agent					178:182	dialdehyde carboxymethyl cellulose (DCMC) tanning agent	128:182	dialdehyde carboxymethyl cellulose (DCMC) tanning agent prepared through periodate oxidation of sodium carboxymethyl cellulose (CMC)	128:259	Formaldehyde was detected in dialdehyde carboxymethyl cellulose (DCMC) tanning agent prepared through periodate oxidation of sodium carboxymethyl cellulose (CMC).
32414428	1	43	located	detected	116:123	arg2	Formaldehyde					99:110	Formaldehyde	99:110	Formaldehyde	99:110	Formaldehyde was detected in dialdehyde carboxymethyl cellulose (DCMC) tanning agent prepared through periodate oxidation of sodium carboxymethyl cellulose (CMC).
32414428	1	43	located	detected	116:123	arg1	agent					178:182	dialdehyde carboxymethyl cellulose (DCMC) tanning agent	128:182	dialdehyde carboxymethyl cellulose (DCMC) tanning agent prepared through periodate oxidation of sodium carboxymethyl cellulose (CMC)	128:259	Formaldehyde was detected in dialdehyde carboxymethyl cellulose (DCMC) tanning agent prepared through periodate oxidation of sodium carboxymethyl cellulose (CMC).
32414428	1	44	theme	DCMC	164:167	arg1	agent					178:182	dialdehyde carboxymethyl cellulose (DCMC) tanning agent	128:182	dialdehyde carboxymethyl cellulose (DCMC) tanning agent prepared through periodate oxidation of sodium carboxymethyl cellulose (CMC)	128:259	Formaldehyde was detected in dialdehyde carboxymethyl cellulose (DCMC) tanning agent prepared through periodate oxidation of sodium carboxymethyl cellulose (CMC).
32414428	6	45	theme	substitution	844:855	arg1	degree					818:823	The low degradation degree	798:823	The low degradation degree	798:823	The low degradation degree and high degree of substitution of CMC reduced the possibility of the formation of reducing glucose residue and glucose during oxidation, thereby resulting in low formaldehyde content in DCMC and DCMC-tanned leather.
32414428	6	45	theme	substitution	844:855	arg1	degree					834:839	high degree	829:839	high degree	829:839	The low degradation degree and high degree of substitution of CMC reduced the possibility of the formation of reducing glucose residue and glucose during oxidation, thereby resulting in low formaldehyde content in DCMC and DCMC-tanned leather.
32414428	1	46	theme	cellulose	153:161	arg1	agent					178:182	dialdehyde carboxymethyl cellulose (DCMC) tanning agent	128:182	dialdehyde carboxymethyl cellulose (DCMC) tanning agent prepared through periodate oxidation of sodium carboxymethyl cellulose (CMC)	128:259	Formaldehyde was detected in dialdehyde carboxymethyl cellulose (DCMC) tanning agent prepared through periodate oxidation of sodium carboxymethyl cellulose (CMC).
32414428	6	47	theme	DCMC-tanned	1021:1031	arg1	leather					1033:1039	DCMC-tanned leather	1021:1039	DCMC-tanned leather	1021:1039	The low degradation degree and high degree of substitution of CMC reduced the possibility of the formation of reducing glucose residue and glucose during oxidation, thereby resulting in low formaldehyde content in DCMC and DCMC-tanned leather.
32414428	5	48	theme	CMC	732:734	arg1	degradation					717:727	the concomitant degradation	701:727	the concomitant degradation of CMC	701:734	Moreover, glucose was produced from the concomitant degradation of CMC during oxidation, and then oxidized to liberate formaldehyde.
32414428	3	49	theme	different	510:518	arg1	degrees					535:541	different polymerization degrees	510:541	different polymerization degrees	510:541	The formation mechanism of formaldehyde in DCMC was investigated by composition analysis and intermediate identification of DCMC with different polymerization degrees and sugar unit structures.
32414428	2	50	theme	potential	344:352	arg1	risk					354:357	a potential risk	342:357	a potential risk to human health	342:373	Formaldehyde was then introduced into leather through DCMC tanning, which poses a potential risk to human health.
32414428	0	51	theme	tanning	84:90	arg1	agent					92:96	dialdehyde carboxymethyl cellulose tanning agent	49:96	dialdehyde carboxymethyl cellulose tanning agent	49:96	Formaldehyde formation during the preparation of dialdehyde carboxymethyl cellulose tanning agent.
32414428	6	52	theme	CMC	860:862	arg1	substitution					844:855	substitution	844:855	substitution of CMC	844:862	The low degradation degree and high degree of substitution of CMC reduced the possibility of the formation of reducing glucose residue and glucose during oxidation, thereby resulting in low formaldehyde content in DCMC and DCMC-tanned leather.
32414428	3	53	theme	unit	553:556	arg1	structures					558:567	sugar unit structures	547:567	sugar unit structures	547:567	The formation mechanism of formaldehyde in DCMC was investigated by composition analysis and intermediate identification of DCMC with different polymerization degrees and sugar unit structures.
32414428	4	54	theme	CMC	660:662	arg1	residue					649:655	the reducing glucose residue	628:655	the reducing glucose residue of CMC	628:662	Formaldehyde was derived from the overoxidation of C-6 on the reducing glucose residue of CMC.
32414428	4	54	theme	CMC	660:662	arg1	CMC					660:662	CMC	660:662	CMC	660:662	Formaldehyde was derived from the overoxidation of C-6 on the reducing glucose residue of CMC.
32414428	0	55	theme	cellulose	74:82	arg1	agent					92:96	dialdehyde carboxymethyl cellulose tanning agent	49:96	dialdehyde carboxymethyl cellulose tanning agent	49:96	Formaldehyde formation during the preparation of dialdehyde carboxymethyl cellulose tanning agent.
34191522	2	0	dep	substantia	468:477	arg1	nigra					479:483	nigra	479:483	nigra	479:483	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	3	1	dep	striatum	762:769	arg1	the					758:760	the	758:760	the	758:760	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	1	2	theme	incidental	243:252	arg1	ILBD					273:276	ILBD	273:276	ILBD	273:276	O-Glycosylation changes in misfolded proteins are of particular interest in understanding neurodegenerative conditions such as Parkinson's disease (PD) and incidental Lewy body disease (ILBD).
34191522	1	2	theme	incidental	243:252	arg1	disease					264:270	incidental Lewy body disease	243:270	incidental Lewy body disease (ILBD)	243:277	O-Glycosylation changes in misfolded proteins are of particular interest in understanding neurodegenerative conditions such as Parkinson's disease (PD) and incidental Lewy body disease (ILBD).
34191522	0	3	from	O-Glycome	4:12	arg1	Disease					78:84	Parkinson's Disease	66:84	Parkinson's Disease	66:84	The O-Glycome of Human Nigrostriatal Tissue and Its Alteration in Parkinson's Disease.
34191522	2	4	gly	O-glycosylation	426:440	arg1	striatum					455:462	human striatum	449:462	human striatum	449:462	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	2	4	gly	O-glycosylation	426:440	arg1	working					527:533	working	527:533	working	527:533	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	2	4	gly	O-glycosylation	426:440	arg1	tissue					485:490	substantia nigra tissue	468:490	substantia nigra tissue	468:490	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	4	5	located	observed	934:941	arg2	increases					881:889	Significant increases	869:889	Significant increases in sialylation (p = 0.038) in PD	869:922	Significant increases in sialylation (p = 0.038) in PD were also observed in the substantia nigra.
34191522	4	5	located	observed	934:941	arg1	nigra					961:965	the substantia nigra	946:965	the substantia nigra	946:965	Significant increases in sialylation (p = 0.038) in PD were also observed in the substantia nigra.
34191522	2	6	theme	release	350:356	arg1	optimizations					299:311	optimizations	299:311	optimizations of a microwave-assisted nonreductive release	299:356	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	5	7	theme	nigrostriatal	1013:1025	arg1	O-glycome					1027:1035	the whole nigrostriatal O-glycome	1003:1035	the whole nigrostriatal O-glycome	1003:1035	This is the first study to profile the whole nigrostriatal O-glycome in healthy, PD, and ILBD tissues, outlining disease biomarkers alongside benefits of employing orthogonal techniques for O-glycan analysis.
34191522	1	8	theme	Lewy	254:257	arg1	ILBD					273:276	ILBD	273:276	ILBD	273:276	O-Glycosylation changes in misfolded proteins are of particular interest in understanding neurodegenerative conditions such as Parkinson's disease (PD) and incidental Lewy body disease (ILBD).
34191522	1	8	theme	Lewy	254:257	arg1	disease					264:270	incidental Lewy body disease	243:270	incidental Lewy body disease (ILBD)	243:277	O-Glycosylation changes in misfolded proteins are of particular interest in understanding neurodegenerative conditions such as Parkinson's disease (PD) and incidental Lewy body disease (ILBD).
34191522	3	9	theme	p	702:702	arg1	0.017					706:710	p = 0.017	702:710	p = 0.017	702:710	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	3	9	theme	p	702:702	arg1	mannose-core					688:699	mannose-core	688:699	mannose-core (p = 0.017)	688:711	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	1	10	theme	body	259:262	arg1	ILBD					273:276	ILBD	273:276	ILBD	273:276	O-Glycosylation changes in misfolded proteins are of particular interest in understanding neurodegenerative conditions such as Parkinson's disease (PD) and incidental Lewy body disease (ILBD).
34191522	1	10	theme	body	259:262	arg1	disease					264:270	incidental Lewy body disease	243:270	incidental Lewy body disease (ILBD)	243:277	O-Glycosylation changes in misfolded proteins are of particular interest in understanding neurodegenerative conditions such as Parkinson's disease (PD) and incidental Lewy body disease (ILBD).
34191522	2	11	theme	healthy	509:515	arg1	controls					517:524	healthy controls	509:524	healthy controls	509:524	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	3	12	theme	glucuronylated	717:730	arg1	0.039					748:752	p = 0.039	744:752	p = 0.039	744:752	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	3	12	theme	glucuronylated	717:730	arg1	structures					732:741	glucuronylated structures	717:741	glucuronylated structures (p = 0.039)	717:753	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	4	13	from	increases	881:889	arg1	sialylation					894:904	sialylation	894:904	sialylation (p = 0.038) in PD	894:922	Significant increases in sialylation (p = 0.038) in PD were also observed in the substantia nigra.
34191522	4	13	from	increases	881:889	arg1	0.038					911:915	p = 0.038	907:915	p = 0.038	907:915	Significant increases in sialylation (p = 0.038) in PD were also observed in the substantia nigra.
34191522	3	14	theme	structures	732:741	arg1	levels					678:683	significantly decreased levels	654:683	significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039)	654:753	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	3	15	from	decrease	834:841	arg1	p					857:857	p = 0.001	857:865	p = 0.001	857:865	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	3	15	from	decrease	834:841	arg1	sulfation					846:854	sulfation	846:854	sulfation (p = 0.001)	846:866	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	3	15	from	decrease	834:841	arg1	sialylation					804:814	sialylation	804:814	sialylation (p < 0.001)	804:826	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	3	15	from	decrease	834:841	arg1	<					819:819	p < 0.001	817:825	p < 0.001	817:825	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	3	16	theme	=	859:859	arg1	p					857:857	p = 0.001	857:865	p = 0.001	857:865	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	3	16	theme	=	859:859	arg1	sulfation					846:854	sulfation	846:854	sulfation (p = 0.001)	846:866	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	2	17	from	tissue	485:490	arg1	ILBD					499:502	ILBD	499:502	ILBD	499:502	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	2	17	from	tissue	485:490	arg1	PD					495:496	PD	495:496	PD	495:496	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	2	17	from	tissue	485:490	arg1	controls					517:524	healthy controls	509:524	healthy controls	509:524	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	1	18	theme	neurodegenerative	177:193	arg1	conditions					195:204	neurodegenerative conditions	177:204	neurodegenerative conditions such as Parkinson's disease (PD) and incidental Lewy body disease (ILBD)	177:277	O-Glycosylation changes in misfolded proteins are of particular interest in understanding neurodegenerative conditions such as Parkinson's disease (PD) and incidental Lewy body disease (ILBD).
34191522	1	18	theme	neurodegenerative	177:193	arg1	disease					264:270	incidental Lewy body disease	243:270	incidental Lewy body disease (ILBD)	243:277	O-Glycosylation changes in misfolded proteins are of particular interest in understanding neurodegenerative conditions such as Parkinson's disease (PD) and incidental Lewy body disease (ILBD).
34191522	1	18	theme	neurodegenerative	177:193	arg1	disease					226:232	Parkinson's disease	214:232	Parkinson's disease (PD)	214:237	O-Glycosylation changes in misfolded proteins are of particular interest in understanding neurodegenerative conditions such as Parkinson's disease (PD) and incidental Lewy body disease (ILBD).
34191522	3	19	theme	p	744:744	arg1	0.039					748:752	p = 0.039	744:752	p = 0.039	744:752	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	3	19	theme	p	744:744	arg1	structures					732:741	glucuronylated structures	717:741	glucuronylated structures (p = 0.039)	717:753	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	2	20	dep	striatum	455:462	arg1	the					445:447	the	445:447	the	445:447	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	4	21	theme	=	909:909	arg1	sialylation					894:904	sialylation	894:904	sialylation (p = 0.038) in PD	894:922	Significant increases in sialylation (p = 0.038) in PD were also observed in the substantia nigra.
34191522	4	21	theme	=	909:909	arg1	0.038					911:915	p = 0.038	907:915	p = 0.038	907:915	Significant increases in sialylation (p = 0.038) in PD were also observed in the substantia nigra.
34191522	2	22	theme	human	449:453	arg1	striatum					455:462	human striatum	449:462	human striatum	449:462	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	2	22	theme	human	449:453	arg1	working					527:533	working	527:533	working	527:533	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	3	23	theme	=	746:746	arg1	0.039					748:752	p = 0.039	744:752	p = 0.039	744:752	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	3	23	theme	=	746:746	arg1	structures					732:741	glucuronylated structures	717:741	glucuronylated structures (p = 0.039)	717:753	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	5	24	theme	healthy	1040:1046	arg1	tissues					1062:1068	healthy, PD, and ILBD tissues	1040:1068	healthy, PD, and ILBD tissues	1040:1068	This is the first study to profile the whole nigrostriatal O-glycome in healthy, PD, and ILBD tissues, outlining disease biomarkers alongside benefits of employing orthogonal techniques for O-glycan analysis.
34191522	0	25	theme	Human	17:21	arg1	Tissue					37:42	Human Nigrostriatal Tissue	17:42	Human Nigrostriatal Tissue	17:42	The O-Glycome of Human Nigrostriatal Tissue and Its Alteration in Parkinson's Disease.
34191522	2	26	theme	well-established	545:560	arg1	approaches					580:589	well-established reductive release approaches	545:589	well-established reductive release approaches	545:589	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	1	27	theme	O-Glycosylation	87:101	arg1	changes					103:109	O-Glycosylation changes	87:109	O-Glycosylation changes in misfolded proteins	87:131	O-Glycosylation changes in misfolded proteins are of particular interest in understanding neurodegenerative conditions such as Parkinson's disease (PD) and incidental Lewy body disease (ILBD).
34191522	0	28	theme	Tissue	37:42	arg1	Alteration					52:61	Its Alteration	48:61	Its Alteration in Parkinson's Disease	48:84	The O-Glycome of Human Nigrostriatal Tissue and Its Alteration in Parkinson's Disease.
34191522	0	28	theme	Tissue	37:42	arg1	O-Glycome					4:12	The O-Glycome	0:12	The O-Glycome of Human Nigrostriatal Tissue	0:42	The O-Glycome of Human Nigrostriatal Tissue and Its Alteration in Parkinson's Disease.
34191522	2	29	theme	microwave-assisted	318:335	arg1	release					350:356	a microwave-assisted nonreductive release	316:356	a microwave-assisted nonreductive release	316:356	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	4	30	theme	Significant	869:879	arg1	increases					881:889	Significant increases	869:889	Significant increases in sialylation (p = 0.038) in PD	869:922	Significant increases in sialylation (p = 0.038) in PD were also observed in the substantia nigra.
34191522	5	31	theme	first	980:984	arg1	This					968:971	This	968:971	This	968:971	This is the first study to profile the whole nigrostriatal O-glycome in healthy, PD, and ILBD tissues, outlining disease biomarkers alongside benefits of employing orthogonal techniques for O-glycan analysis.
34191522	5	31	theme	first	980:984	arg1	study					986:990	the first study	976:990	the first study to profile the whole nigrostriatal O-glycome in healthy, PD, and ILBD tissues, outlining disease biomarkers alongside benefits of employing orthogonal techniques for O-glycan analysis	976:1174	This is the first study to profile the whole nigrostriatal O-glycome in healthy, PD, and ILBD tissues, outlining disease biomarkers alongside benefits of employing orthogonal techniques for O-glycan analysis.
34191522	4	32	gly	sialylation	894:904	arg1	PD					921:922	PD	921:922	PD	921:922	Significant increases in sialylation (p = 0.038) in PD were also observed in the substantia nigra.
34191522	0	33	theme	Nigrostriatal	23:35	arg1	Tissue					37:42	Human Nigrostriatal Tissue	17:42	Human Nigrostriatal Tissue	17:42	The O-Glycome of Human Nigrostriatal Tissue and Its Alteration in Parkinson's Disease.
34191522	5	34	theme	PD	1049:1050	arg1	tissues					1062:1068	healthy, PD, and ILBD tissues	1040:1068	healthy, PD, and ILBD tissues	1040:1068	This is the first study to profile the whole nigrostriatal O-glycome in healthy, PD, and ILBD tissues, outlining disease biomarkers alongside benefits of employing orthogonal techniques for O-glycan analysis.
34191522	4	35	from	sialylation	894:904	arg1	PD					921:922	PD	921:922	PD	921:922	Significant increases in sialylation (p = 0.038) in PD were also observed in the substantia nigra.
34191522	5	36	dep	employing	1122:1130	arg1	benefits					1110:1117	benefits	1110:1117	benefits	1110:1117	This is the first study to profile the whole nigrostriatal O-glycome in healthy, PD, and ILBD tissues, outlining disease biomarkers alongside benefits of employing orthogonal techniques for O-glycan analysis.
34191522	4	37	theme	substantia	950:959	arg1	nigra					961:965	the substantia nigra	946:965	the substantia nigra	946:965	Significant increases in sialylation (p = 0.038) in PD were also observed in the substantia nigra.
34191522	5	38	theme	disease	1081:1087	arg1	biomarkers					1089:1098	disease biomarkers	1081:1098	disease biomarkers	1081:1098	This is the first study to profile the whole nigrostriatal O-glycome in healthy, PD, and ILBD tissues, outlining disease biomarkers alongside benefits of employing orthogonal techniques for O-glycan analysis.
34191522	2	39	theme	substantia	468:477	arg1	tissue					485:490	substantia nigra tissue	468:490	substantia nigra tissue	468:490	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	0	40	from	Alteration	52:61	arg1	Disease					78:84	Parkinson's Disease	66:84	Parkinson's Disease	66:84	The O-Glycome of Human Nigrostriatal Tissue and Its Alteration in Parkinson's Disease.
34191522	5	41	theme	whole	1007:1011	arg1	O-glycome					1027:1035	the whole nigrostriatal O-glycome	1003:1035	the whole nigrostriatal O-glycome	1003:1035	This is the first study to profile the whole nigrostriatal O-glycome in healthy, PD, and ILBD tissues, outlining disease biomarkers alongside benefits of employing orthogonal techniques for O-glycan analysis.
34191522	2	42	theme	nonreductive	337:348	arg1	release					350:356	a microwave-assisted nonreductive release	316:356	a microwave-assisted nonreductive release	316:356	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	3	43	from	increase	792:799	arg1	p					857:857	p = 0.001	857:865	p = 0.001	857:865	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	3	43	from	increase	792:799	arg1	sulfation					846:854	sulfation	846:854	sulfation (p = 0.001)	846:866	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	3	43	from	increase	792:799	arg1	sialylation					804:814	sialylation	804:814	sialylation (p < 0.001)	804:826	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	3	43	from	increase	792:799	arg1	<					819:819	p < 0.001	817:825	p < 0.001	817:825	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	1	44	theme	misfolded	114:122	arg1	proteins					124:131	misfolded proteins	114:131	misfolded proteins	114:131	O-Glycosylation changes in misfolded proteins are of particular interest in understanding neurodegenerative conditions such as Parkinson's disease (PD) and incidental Lewy body disease (ILBD).
34191522	5	45	theme	ILBD	1057:1060	arg1	tissues					1062:1068	healthy, PD, and ILBD tissues	1040:1068	healthy, PD, and ILBD tissues	1040:1068	This is the first study to profile the whole nigrostriatal O-glycome in healthy, PD, and ILBD tissues, outlining disease biomarkers alongside benefits of employing orthogonal techniques for O-glycan analysis.
34191522	2	46	theme	release	572:578	arg1	approaches					580:589	well-established reductive release approaches	545:589	well-established reductive release approaches	545:589	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	3	47	theme	mannose-core	688:699	arg1	levels					678:683	significantly decreased levels	654:683	significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039)	654:753	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	1	48	from	changes	103:109	arg1	proteins					124:131	misfolded proteins	114:131	misfolded proteins	114:131	O-Glycosylation changes in misfolded proteins are of particular interest in understanding neurodegenerative conditions such as Parkinson's disease (PD) and incidental Lewy body disease (ILBD).
34191522	2	49	theme	reductive	562:570	arg1	approaches					580:589	well-established reductive release approaches	545:589	well-established reductive release approaches	545:589	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	3	50	theme	decreased	668:676	arg1	levels					678:683	significantly decreased levels	654:683	significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039)	654:753	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	3	51	theme	p	817:817	arg1	sialylation					804:814	sialylation	804:814	sialylation (p < 0.001)	804:826	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	3	51	theme	p	817:817	arg1	<					819:819	p < 0.001	817:825	p < 0.001	817:825	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	5	52	theme	orthogonal	1132:1141	arg1	techniques					1143:1152	orthogonal techniques	1132:1152	orthogonal techniques	1132:1152	This is the first study to profile the whole nigrostriatal O-glycome in healthy, PD, and ILBD tissues, outlining disease biomarkers alongside benefits of employing orthogonal techniques for O-glycan analysis.
34191522	2	53	from	striatum	455:462	arg1	ILBD					499:502	ILBD	499:502	ILBD	499:502	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	2	53	from	striatum	455:462	arg1	PD					495:496	PD	495:496	PD	495:496	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	2	53	from	striatum	455:462	arg1	controls					517:524	healthy controls	509:524	healthy controls	509:524	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	5	54	from	profile	995:1001	arg1	tissues					1062:1068	healthy, PD, and ILBD tissues	1040:1068	healthy, PD, and ILBD tissues	1040:1068	This is the first study to profile the whole nigrostriatal O-glycome in healthy, PD, and ILBD tissues, outlining disease biomarkers alongside benefits of employing orthogonal techniques for O-glycan analysis.
34191522	3	55	theme	O-glycans	606:614	arg1	total					594:598	A total	592:598	A total of 70 O-glycans	592:614	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	2	56	theme	glycan	367:372	arg1	degradation					374:384	glycan degradation	367:384	glycan degradation	367:384	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	4	57	theme	p	907:907	arg1	sialylation					894:904	sialylation	894:904	sialylation (p = 0.038) in PD	894:922	Significant increases in sialylation (p = 0.038) in PD were also observed in the substantia nigra.
34191522	4	57	theme	p	907:907	arg1	0.038					911:915	p = 0.038	907:915	p = 0.038	907:915	Significant increases in sialylation (p = 0.038) in PD were also observed in the substantia nigra.
34191522	3	58	theme	=	704:704	arg1	0.017					706:710	p = 0.017	702:710	p = 0.017	702:710	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	3	58	theme	=	704:704	arg1	mannose-core					688:699	mannose-core	688:699	mannose-core (p = 0.017)	688:711	A total of 70 O-glycans were identified, with ILBD presenting significantly decreased levels of mannose-core (p = 0.017) and glucuronylated structures (p = 0.039) in the striatum and PD presenting an increase in sialylation (p < 0.001) and a decrease in sulfation (p = 0.001).
34191522	2	59	from	O-glycosylation	426:440	arg1	striatum					455:462	human striatum	449:462	human striatum	449:462	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	2	59	from	O-glycosylation	426:440	arg1	working					527:533	working	527:533	working	527:533	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	2	59	from	O-glycosylation	426:440	arg1	tissue					485:490	substantia nigra tissue	468:490	substantia nigra tissue	468:490	This work outlines optimizations of a microwave-assisted nonreductive release to limit glycan degradation and employs this methodology to analyze O-glycosylation on the human striatum and substantia nigra tissue in PD, ILBD, and healthy controls, working alongside well-established reductive release approaches.
34191522	1	60	theme	particular	140:149	arg1	interest					151:158	particular interest	140:158	particular interest	140:158	O-Glycosylation changes in misfolded proteins are of particular interest in understanding neurodegenerative conditions such as Parkinson's disease (PD) and incidental Lewy body disease (ILBD).
34191522	5	61	theme	O-glycan	1158:1165	arg1	analysis					1167:1174	O-glycan analysis	1158:1174	O-glycan analysis	1158:1174	This is the first study to profile the whole nigrostriatal O-glycome in healthy, PD, and ILBD tissues, outlining disease biomarkers alongside benefits of employing orthogonal techniques for O-glycan analysis.
34224572	7	0	theme	FODMAPs	1278:1284	arg1	intake					1253:1258	a mean intake	1246:1258	a mean intake of 18.9 ± 9.5 g/d FODMAPs in this French cohort	1246:1306	RESULTS We observed a mean intake of 18.9 ± 9.5 g/d FODMAPs in this French cohort, and 11.7% of participants had intakes <9 g/d (i.e., low-FODMAP diets).
34224572	1	1	theme	bowel	272:276	arg1	syndrome					278:285	irritable bowel syndrome	262:285	irritable bowel syndrome in particular	262:299	BACKGROUND Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) are increasingly studied because they are suspected unfavorably to impact health (irritable bowel syndrome in particular).
34224572	8	2	theme	lower	1590:1594	arg1	levels					1596:1601	lower levels	1590:1601	lower levels of physical activity	1590:1622	Participants with FODMAP intakes <9 g/d were more likely to have lower caloric intakes (Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d), to be smokers, to have lower incomes, and to have lower levels of physical activity.
34224572	3	3	dep	OBJECTIVES	490:499	arg1	aimed					512:516	aimed	512:516	aimed to describe FODMAP consumption in a large French cohort and its association with sociodemographic and lifestyle characteristics	512:644	OBJECTIVES This study aimed to describe FODMAP consumption in a large French cohort and its association with sociodemographic and lifestyle characteristics.
34224572	4	4	theme	composition	830:840	arg1	table					842:846	an ad hoc FODMAP composition table	813:846	an ad hoc FODMAP composition table	813:846	METHODS This cross-sectional study described FODMAP intakes in 109,362 volunteers (78.0% female, mean age 43.8 ± 14.7 y) from the French NutriNet-Santé cohort, using an ad hoc FODMAP composition table.
34224572	2	5	from	intake	341:346	arg1	population					363:372	the general population	351:372	the general population	351:372	However, little is known about FODMAP intake in the general population, or which groups are more likely to consume them, because their intakes are usually assessed in inpatient settings.
34224572	11	6	theme	general	1935:1941	arg1	population					1943:1952	the general population	1931:1952	the general population	1931:1952	CONCLUSIONS FODMAP consumption by a large sample of adults from the general population is ∼19 g/d, with half of the population having a FODMAP intake >16 g/d.
34224572	6	7	theme	24-h	1207:1210	arg1	records					1217:1223	detailed 24-h food records	1198:1223	detailed 24-h food records	1198:1223	Eligible participants had completed ≥3 detailed 24-h food records.
34224572	4	8	theme	FODMAP	692:697	arg1	intakes					699:705	FODMAP intakes	692:705	FODMAP intakes	692:705	METHODS This cross-sectional study described FODMAP intakes in 109,362 volunteers (78.0% female, mean age 43.8 ± 14.7 y) from the French NutriNet-Santé cohort, using an ad hoc FODMAP composition table.
34224572	3	9	theme	French	560:565	arg1	cohort					567:572	a large French cohort	552:572	a large French cohort	552:572	OBJECTIVES This study aimed to describe FODMAP consumption in a large French cohort and its association with sociodemographic and lifestyle characteristics.
34224572	9	10	theme	mean	1655:1658	arg1	intake					1660:1665	a mean intake	1653:1665	a mean intake	1653:1665	Total FODMAPs accounted for a mean intake of 18.9 ± 9.5 g/d, which was 3.7 ± 2.0% of total energy intake.
34224572	9	10	theme	mean	1655:1658	arg1	intake					1723:1728	total energy intake	1710:1728	total energy intake	1710:1728	Total FODMAPs accounted for a mean intake of 18.9 ± 9.5 g/d, which was 3.7 ± 2.0% of total energy intake.
34224572	9	10	theme	mean	1655:1658	arg1	%					1705:1705	3.7 ± 2.0%	1696:1705	3.7 ± 2.0% of total energy intake	1696:1728	Total FODMAPs accounted for a mean intake of 18.9 ± 9.5 g/d, which was 3.7 ± 2.0% of total energy intake.
34224572	7	11	contain	had	1335:1337	arg1	participants					1322:1333	participants	1322:1333	participants	1322:1333	RESULTS We observed a mean intake of 18.9 ± 9.5 g/d FODMAPs in this French cohort, and 11.7% of participants had intakes <9 g/d (i.e., low-FODMAP diets).
34224572	7	11	contain	had	1335:1337	arg1	%					1317:1317	11.7%	1313:1317	11.7% of participants	1313:1333	RESULTS We observed a mean intake of 18.9 ± 9.5 g/d FODMAPs in this French cohort, and 11.7% of participants had intakes <9 g/d (i.e., low-FODMAP diets).
34224572	7	11	contain	had	1335:1337	arg2	g/d					1350:1352	intakes <9 g/d	1339:1352	intakes <9 g/d	1339:1352	RESULTS We observed a mean intake of 18.9 ± 9.5 g/d FODMAPs in this French cohort, and 11.7% of participants had intakes <9 g/d (i.e., low-FODMAP diets).
34224572	10	12	theme	highest	1735:1741	arg1	intake					1743:1748	The highest intake	1731:1748	The highest intake of FODMAPs	1731:1759	The highest intake of FODMAPs was represented by lactose followed by excess fructose, fructans, polyols, and galacto-oligo-saccharides.
34224572	7	13	dep	diets	1372:1376	arg1	i.e.					1355:1358	i.e.	1355:1358	i.e.	1355:1358	RESULTS We observed a mean intake of 18.9 ± 9.5 g/d FODMAPs in this French cohort, and 11.7% of participants had intakes <9 g/d (i.e., low-FODMAP diets).
34224572	11	14	theme	FODMAP	2003:2008	arg1	g/d					2021:2023	a FODMAP intake >16 g/d	2001:2023	a FODMAP intake >16 g/d	2001:2023	CONCLUSIONS FODMAP consumption by a large sample of adults from the general population is ∼19 g/d, with half of the population having a FODMAP intake >16 g/d.
34224572	8	15	theme	caloric	1451:1457	arg1	g/d					1534:1536	Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d	1468:1536	Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d	1468:1536	Participants with FODMAP intakes <9 g/d were more likely to have lower caloric intakes (Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d), to be smokers, to have lower incomes, and to have lower levels of physical activity.
34224572	8	15	theme	caloric	1451:1457	arg1	intakes					1459:1465	lower caloric intakes	1445:1465	lower caloric intakes (Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d)	1445:1537	Participants with FODMAP intakes <9 g/d were more likely to have lower caloric intakes (Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d), to be smokers, to have lower incomes, and to have lower levels of physical activity.
34224572	4	16	theme	78.0	730:733	arg1	%					734:734	%	734:734	%	734:734	METHODS This cross-sectional study described FODMAP intakes in 109,362 volunteers (78.0% female, mean age 43.8 ± 14.7 y) from the French NutriNet-Santé cohort, using an ad hoc FODMAP composition table.
34224572	5	17	theme	logistic	1064:1071	arg1	regressions					1073:1083	multinomial logistic regressions	1052:1083	multinomial logistic regressions	1052:1083	Associations between FODMAP intakes and sociodemographic characteristics were investigated using χ2 tests or Kruskal-Wallis tests according to the qualitative or quantitative status of the variable, and multinomial logistic regressions were performed after adjusting for energy intake in sensitivity analyses.
34224572	4	18	dep	age	749:751	arg1	y					765:765	y	765:765	mean age 43.8 ± 14.7 y	744:765	METHODS This cross-sectional study described FODMAP intakes in 109,362 volunteers (78.0% female, mean age 43.8 ± 14.7 y) from the French NutriNet-Santé cohort, using an ad hoc FODMAP composition table.
34224572	9	19	theme	g/d	1681:1683	arg1	intake					1660:1665	a mean intake	1653:1665	a mean intake	1653:1665	Total FODMAPs accounted for a mean intake of 18.9 ± 9.5 g/d, which was 3.7 ± 2.0% of total energy intake.
34224572	9	19	theme	g/d	1681:1683	arg1	intake					1723:1728	total energy intake	1710:1728	total energy intake	1710:1728	Total FODMAPs accounted for a mean intake of 18.9 ± 9.5 g/d, which was 3.7 ± 2.0% of total energy intake.
34224572	9	19	theme	g/d	1681:1683	arg1	%					1705:1705	3.7 ± 2.0%	1696:1705	3.7 ± 2.0% of total energy intake	1696:1728	Total FODMAPs accounted for a mean intake of 18.9 ± 9.5 g/d, which was 3.7 ± 2.0% of total energy intake.
34224572	7	20	theme	low-FODMAP	1361:1370	arg1	diets					1372:1376	low-FODMAP diets	1361:1376	low-FODMAP diets	1361:1376	RESULTS We observed a mean intake of 18.9 ± 9.5 g/d FODMAPs in this French cohort, and 11.7% of participants had intakes <9 g/d (i.e., low-FODMAP diets).
34224572	8	21	theme	lower	1445:1449	arg1	g/d					1534:1536	Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d	1468:1536	Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d	1468:1536	Participants with FODMAP intakes <9 g/d were more likely to have lower caloric intakes (Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d), to be smokers, to have lower incomes, and to have lower levels of physical activity.
34224572	8	21	theme	lower	1445:1449	arg1	intakes					1459:1465	lower caloric intakes	1445:1465	lower caloric intakes (Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d)	1445:1537	Participants with FODMAP intakes <9 g/d were more likely to have lower caloric intakes (Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d), to be smokers, to have lower incomes, and to have lower levels of physical activity.
34224572	3	22	theme	lifestyle	620:628	arg1	characteristics					630:644	sociodemographic and lifestyle characteristics	599:644	sociodemographic and lifestyle characteristics	599:644	OBJECTIVES This study aimed to describe FODMAP consumption in a large French cohort and its association with sociodemographic and lifestyle characteristics.
34224572	8	23	theme	=	1470:1470	arg1	g/d					1534:1536	Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d	1468:1536	Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d	1468:1536	Participants with FODMAP intakes <9 g/d were more likely to have lower caloric intakes (Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d), to be smokers, to have lower incomes, and to have lower levels of physical activity.
34224572	8	23	theme	=	1470:1470	arg1	intakes					1459:1465	lower caloric intakes	1445:1465	lower caloric intakes (Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d)	1445:1537	Participants with FODMAP intakes <9 g/d were more likely to have lower caloric intakes (Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d), to be smokers, to have lower incomes, and to have lower levels of physical activity.
34224572	7	24	theme	g/d	1274:1276	arg1	FODMAPs					1278:1284	18.9 ± 9.5 g/d FODMAPs	1263:1284	18.9 ± 9.5 g/d FODMAPs	1263:1284	RESULTS We observed a mean intake of 18.9 ± 9.5 g/d FODMAPs in this French cohort, and 11.7% of participants had intakes <9 g/d (i.e., low-FODMAP diets).
34224572	3	25	theme	sociodemographic	599:614	arg1	characteristics					630:644	sociodemographic and lifestyle characteristics	599:644	sociodemographic and lifestyle characteristics	599:644	OBJECTIVES This study aimed to describe FODMAP consumption in a large French cohort and its association with sociodemographic and lifestyle characteristics.
34224572	5	26	theme	sociodemographic	889:904	arg1	characteristics					906:920	sociodemographic characteristics	889:920	sociodemographic characteristics	889:920	Associations between FODMAP intakes and sociodemographic characteristics were investigated using χ2 tests or Kruskal-Wallis tests according to the qualitative or quantitative status of the variable, and multinomial logistic regressions were performed after adjusting for energy intake in sensitivity analyses.
34224572	2	27	theme	inpatient	470:478	arg1	settings					480:487	inpatient settings	470:487	inpatient settings	470:487	However, little is known about FODMAP intake in the general population, or which groups are more likely to consume them, because their intakes are usually assessed in inpatient settings.
34224572	3	28	from	consumption	537:547	arg1	cohort					567:572	a large French cohort	552:572	a large French cohort	552:572	OBJECTIVES This study aimed to describe FODMAP consumption in a large French cohort and its association with sociodemographic and lifestyle characteristics.
34224572	7	29	theme	mean	1248:1251	arg1	intake					1253:1258	a mean intake	1246:1258	a mean intake of 18.9 ± 9.5 g/d FODMAPs in this French cohort	1246:1306	RESULTS We observed a mean intake of 18.9 ± 9.5 g/d FODMAPs in this French cohort, and 11.7% of participants had intakes <9 g/d (i.e., low-FODMAP diets).
34224572	9	30	theme	energy	1716:1721	arg1	intake					1723:1728	total energy intake	1710:1728	total energy intake	1710:1728	Total FODMAPs accounted for a mean intake of 18.9 ± 9.5 g/d, which was 3.7 ± 2.0% of total energy intake.
34224572	8	31	with	participants	1497:1508	arg1	intakes					1522:1528	FODMAP intakes	1515:1528	FODMAP intakes	1515:1528	Participants with FODMAP intakes <9 g/d were more likely to have lower caloric intakes (Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d), to be smokers, to have lower incomes, and to have lower levels of physical activity.
34224572	3	32	with	association	582:592	arg1	characteristics					630:644	sociodemographic and lifestyle characteristics	599:644	sociodemographic and lifestyle characteristics	599:644	OBJECTIVES This study aimed to describe FODMAP consumption in a large French cohort and its association with sociodemographic and lifestyle characteristics.
34224572	5	33	theme	sensitivity	1137:1147	arg1	analyses					1149:1156	sensitivity analyses	1137:1156	sensitivity analyses	1137:1156	Associations between FODMAP intakes and sociodemographic characteristics were investigated using χ2 tests or Kruskal-Wallis tests according to the qualitative or quantitative status of the variable, and multinomial logistic regressions were performed after adjusting for energy intake in sensitivity analyses.
34224572	1	34	theme	BACKGROUND	85:94	arg1	oligosaccharides					108:123	BACKGROUND Fermentable oligosaccharides	85:123	BACKGROUND Fermentable oligosaccharides	85:123	BACKGROUND Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) are increasingly studied because they are suspected unfavorably to impact health (irritable bowel syndrome in particular).
34224572	1	34	theme	BACKGROUND	85:94	arg1	FODMAPs					171:177	FODMAPs	171:177	FODMAPs	171:177	BACKGROUND Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) are increasingly studied because they are suspected unfavorably to impact health (irritable bowel syndrome in particular).
34224572	4	35	theme	mean	744:747	arg1	age					749:751	mean age 43.8 ± 14.7	744:763	mean age 43.8 ± 14.7 y	744:765	METHODS This cross-sectional study described FODMAP intakes in 109,362 volunteers (78.0% female, mean age 43.8 ± 14.7 y) from the French NutriNet-Santé cohort, using an ad hoc FODMAP composition table.
34224572	4	35	theme	mean	744:747	arg1	female					736:741	78.0% female	730:741	78.0% female	730:741	METHODS This cross-sectional study described FODMAP intakes in 109,362 volunteers (78.0% female, mean age 43.8 ± 14.7 y) from the French NutriNet-Santé cohort, using an ad hoc FODMAP composition table.
34224572	11	36	with	g/d	1961:1963	arg1	half					1971:1974	half	1971:1974	half of the population having a FODMAP intake >16 g/d	1971:2023	CONCLUSIONS FODMAP consumption by a large sample of adults from the general population is ∼19 g/d, with half of the population having a FODMAP intake >16 g/d.
34224572	5	37	theme	Kruskal-Wallis	958:971	arg1	tests					973:977	Kruskal-Wallis tests	958:977	Kruskal-Wallis tests	958:977	Associations between FODMAP intakes and sociodemographic characteristics were investigated using χ2 tests or Kruskal-Wallis tests according to the qualitative or quantitative status of the variable, and multinomial logistic regressions were performed after adjusting for energy intake in sensitivity analyses.
34224572	11	38	theme	CONCLUSIONS	1867:1877	arg1	consumption					1886:1896	CONCLUSIONS FODMAP consumption	1867:1896	CONCLUSIONS FODMAP consumption by a large sample of adults from the general population	1867:1952	CONCLUSIONS FODMAP consumption by a large sample of adults from the general population is ∼19 g/d, with half of the population having a FODMAP intake >16 g/d.
34224572	3	39	from	association	582:592	arg1	cohort					567:572	a large French cohort	552:572	a large French cohort	552:572	OBJECTIVES This study aimed to describe FODMAP consumption in a large French cohort and its association with sociodemographic and lifestyle characteristics.
34224572	4	40	dep	METHODS	647:653	arg1	described					682:690	described	682:690	described	682:690	METHODS This cross-sectional study described FODMAP intakes in 109,362 volunteers (78.0% female, mean age 43.8 ± 14.7 y) from the French NutriNet-Santé cohort, using an ad hoc FODMAP composition table.
34224572	0	41	theme	NutriNet-Santé	62:75	arg1	Cohort					77:82	the French Population-Based NutriNet-Santé Cohort	34:82	the French Population-Based NutriNet-Santé Cohort	34:82	FODMAP Consumption by Adults from the French Population-Based NutriNet-Santé Cohort.
34224572	4	42	theme	hoc	819:821	arg1	table					842:846	an ad hoc FODMAP composition table	813:846	an ad hoc FODMAP composition table	813:846	METHODS This cross-sectional study described FODMAP intakes in 109,362 volunteers (78.0% female, mean age 43.8 ± 14.7 y) from the French NutriNet-Santé cohort, using an ad hoc FODMAP composition table.
34224572	3	43	with	consumption	537:547	arg1	characteristics					630:644	sociodemographic and lifestyle characteristics	599:644	sociodemographic and lifestyle characteristics	599:644	OBJECTIVES This study aimed to describe FODMAP consumption in a large French cohort and its association with sociodemographic and lifestyle characteristics.
34224572	5	44	theme	multinomial	1052:1062	arg1	regressions					1073:1083	multinomial logistic regressions	1052:1083	multinomial logistic regressions	1052:1083	Associations between FODMAP intakes and sociodemographic characteristics were investigated using χ2 tests or Kruskal-Wallis tests according to the qualitative or quantitative status of the variable, and multinomial logistic regressions were performed after adjusting for energy intake in sensitivity analyses.
34224572	4	45	theme	NutriNet-Santé	784:797	arg1	cohort					799:804	the French NutriNet-Santé cohort	773:804	the French NutriNet-Santé cohort	773:804	METHODS This cross-sectional study described FODMAP intakes in 109,362 volunteers (78.0% female, mean age 43.8 ± 14.7 y) from the French NutriNet-Santé cohort, using an ad hoc FODMAP composition table.
34224572	2	46	theme	FODMAP	334:339	arg1	intake					341:346	FODMAP intake	334:346	FODMAP intake in the general population	334:372	However, little is known about FODMAP intake in the general population, or which groups are more likely to consume them, because their intakes are usually assessed in inpatient settings.
34224572	10	47	theme	excess	1800:1805	arg1	fructose					1807:1814	excess fructose	1800:1814	excess fructose	1800:1814	The highest intake of FODMAPs was represented by lactose followed by excess fructose, fructans, polyols, and galacto-oligo-saccharides.
34224572	10	47	theme	excess	1800:1805	arg1	polyols					1827:1833	polyols	1827:1833	polyols	1827:1833	The highest intake of FODMAPs was represented by lactose followed by excess fructose, fructans, polyols, and galacto-oligo-saccharides.
34224572	10	47	theme	excess	1800:1805	arg1	galacto-oligo-saccharides					1840:1864	galacto-oligo-saccharides	1840:1864	galacto-oligo-saccharides	1840:1864	The highest intake of FODMAPs was represented by lactose followed by excess fructose, fructans, polyols, and galacto-oligo-saccharides.
34224572	10	47	theme	excess	1800:1805	arg1	fructans					1817:1824	fructans	1817:1824	fructans	1817:1824	The highest intake of FODMAPs was represented by lactose followed by excess fructose, fructans, polyols, and galacto-oligo-saccharides.
34224572	8	48	theme	physical	1606:1613	arg1	activity					1615:1622	physical activity	1606:1622	physical activity	1606:1622	Participants with FODMAP intakes <9 g/d were more likely to have lower caloric intakes (Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d), to be smokers, to have lower incomes, and to have lower levels of physical activity.
34224572	7	49	dep	had	1335:1337	arg1	diets					1372:1376	low-FODMAP diets	1361:1376	low-FODMAP diets	1361:1376	RESULTS We observed a mean intake of 18.9 ± 9.5 g/d FODMAPs in this French cohort, and 11.7% of participants had intakes <9 g/d (i.e., low-FODMAP diets).
34224572	7	50	theme	French	1294:1299	arg1	cohort					1301:1306	this French cohort	1289:1306	this French cohort	1289:1306	RESULTS We observed a mean intake of 18.9 ± 9.5 g/d FODMAPs in this French cohort, and 11.7% of participants had intakes <9 g/d (i.e., low-FODMAP diets).
34224572	2	51	theme	general	355:361	arg1	population					363:372	the general population	351:372	the general population	351:372	However, little is known about FODMAP intake in the general population, or which groups are more likely to consume them, because their intakes are usually assessed in inpatient settings.
34224572	1	52	theme	irritable	262:270	arg1	syndrome					278:285	irritable bowel syndrome	262:285	irritable bowel syndrome in particular	262:299	BACKGROUND Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) are increasingly studied because they are suspected unfavorably to impact health (irritable bowel syndrome in particular).
34224572	11	53	from	population	1943:1952	arg1	adults					1919:1924	adults	1919:1924	adults from the general population	1919:1952	CONCLUSIONS FODMAP consumption by a large sample of adults from the general population is ∼19 g/d, with half of the population having a FODMAP intake >16 g/d.
34224572	11	53	from	population	1943:1952	arg1	sample					1909:1914	a large sample	1901:1914	a large sample of adults from the general population	1901:1952	CONCLUSIONS FODMAP consumption by a large sample of adults from the general population is ∼19 g/d, with half of the population having a FODMAP intake >16 g/d.
34224572	3	54	theme	FODMAP	530:535	arg1	consumption					537:547	FODMAP consumption	530:547	FODMAP consumption in a large French cohort	530:572	OBJECTIVES This study aimed to describe FODMAP consumption in a large French cohort and its association with sociodemographic and lifestyle characteristics.
34224572	11	55	from	sample	1909:1914	arg1	population					1943:1952	the general population	1931:1952	the general population	1931:1952	CONCLUSIONS FODMAP consumption by a large sample of adults from the general population is ∼19 g/d, with half of the population having a FODMAP intake >16 g/d.
34224572	0	56	theme	French	38:43	arg1	Cohort					77:82	the French Population-Based NutriNet-Santé Cohort	34:82	the French Population-Based NutriNet-Santé Cohort	34:82	FODMAP Consumption by Adults from the French Population-Based NutriNet-Santé Cohort.
34224572	4	57	theme	FODMAP	823:828	arg1	table					842:846	an ad hoc FODMAP composition table	813:846	an ad hoc FODMAP composition table	813:846	METHODS This cross-sectional study described FODMAP intakes in 109,362 volunteers (78.0% female, mean age 43.8 ± 14.7 y) from the French NutriNet-Santé cohort, using an ad hoc FODMAP composition table.
34224572	6	58	theme	food	1212:1215	arg1	records					1217:1223	detailed 24-h food records	1198:1223	detailed 24-h food records	1198:1223	Eligible participants had completed ≥3 detailed 24-h food records.
34224572	4	59	theme	cross-sectional	660:674	arg1	study					676:680	This cross-sectional study	655:680	This cross-sectional study	655:680	METHODS This cross-sectional study described FODMAP intakes in 109,362 volunteers (78.0% female, mean age 43.8 ± 14.7 y) from the French NutriNet-Santé cohort, using an ad hoc FODMAP composition table.
34224572	3	60	theme	large	554:558	arg1	cohort					567:572	a large French cohort	552:572	a large French cohort	552:572	OBJECTIVES This study aimed to describe FODMAP consumption in a large French cohort and its association with sociodemographic and lifestyle characteristics.
34224572	5	61	theme	FODMAP	870:875	arg1	intakes					877:883	FODMAP intakes	870:883	FODMAP intakes	870:883	Associations between FODMAP intakes and sociodemographic characteristics were investigated using χ2 tests or Kruskal-Wallis tests according to the qualitative or quantitative status of the variable, and multinomial logistic regressions were performed after adjusting for energy intake in sensitivity analyses.
34224572	6	62	theme	detailed	1198:1205	arg1	records					1217:1223	detailed 24-h food records	1198:1223	detailed 24-h food records	1198:1223	Eligible participants had completed ≥3 detailed 24-h food records.
34224572	1	63	from	syndrome	278:285	arg1	particular					290:299	particular	290:299	particular	290:299	BACKGROUND Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) are increasingly studied because they are suspected unfavorably to impact health (irritable bowel syndrome in particular).
34224572	11	64	theme	∼19	1957:1959	arg1	g/d					1961:1963	∼19 g/d	1957:1963	∼19 g/d	1957:1963	CONCLUSIONS FODMAP consumption by a large sample of adults from the general population is ∼19 g/d, with half of the population having a FODMAP intake >16 g/d.
34224572	11	65	theme	population	1983:1992	arg1	half					1971:1974	half	1971:1974	half of the population having a FODMAP intake >16 g/d	1971:2023	CONCLUSIONS FODMAP consumption by a large sample of adults from the general population is ∼19 g/d, with half of the population having a FODMAP intake >16 g/d.
34224572	1	66	dep	health	254:259	arg1	syndrome					278:285	irritable bowel syndrome	262:285	irritable bowel syndrome in particular	262:299	BACKGROUND Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) are increasingly studied because they are suspected unfavorably to impact health (irritable bowel syndrome in particular).
34224572	0	67	from	Cohort	77:82	arg1	Adults					22:27	Adults	22:27	Adults from the French Population-Based NutriNet-Santé Cohort	22:82	FODMAP Consumption by Adults from the French Population-Based NutriNet-Santé Cohort.
34224572	5	68	theme	energy	1120:1125	arg1	intake					1127:1132	energy intake	1120:1132	energy intake in sensitivity analyses	1120:1156	Associations between FODMAP intakes and sociodemographic characteristics were investigated using χ2 tests or Kruskal-Wallis tests according to the qualitative or quantitative status of the variable, and multinomial logistic regressions were performed after adjusting for energy intake in sensitivity analyses.
34224572	11	69	theme	intake	2010:2015	arg1	g/d					2021:2023	a FODMAP intake >16 g/d	2001:2023	a FODMAP intake >16 g/d	2001:2023	CONCLUSIONS FODMAP consumption by a large sample of adults from the general population is ∼19 g/d, with half of the population having a FODMAP intake >16 g/d.
34224572	7	70	from	intake	1253:1258	arg1	cohort					1301:1306	this French cohort	1289:1306	this French cohort	1289:1306	RESULTS We observed a mean intake of 18.9 ± 9.5 g/d FODMAPs in this French cohort, and 11.7% of participants had intakes <9 g/d (i.e., low-FODMAP diets).
34224572	11	71	contain	having	1994:1999	arg1	population					1983:1992	the population	1979:1992	the population having a FODMAP intake >16 g/d	1979:2023	CONCLUSIONS FODMAP consumption by a large sample of adults from the general population is ∼19 g/d, with half of the population having a FODMAP intake >16 g/d.
34224572	11	71	contain	having	1994:1999	arg2	g/d					2021:2023	a FODMAP intake >16 g/d	2001:2023	a FODMAP intake >16 g/d	2001:2023	CONCLUSIONS FODMAP consumption by a large sample of adults from the general population is ∼19 g/d, with half of the population having a FODMAP intake >16 g/d.
34224572	11	72	theme	adults	1919:1924	arg1	sample					1909:1914	a large sample	1901:1914	a large sample of adults from the general population	1901:1952	CONCLUSIONS FODMAP consumption by a large sample of adults from the general population is ∼19 g/d, with half of the population having a FODMAP intake >16 g/d.
34224572	5	73	theme	χ2	946:947	arg1	tests					949:953	χ2 tests	946:953	χ2 tests	946:953	Associations between FODMAP intakes and sociodemographic characteristics were investigated using χ2 tests or Kruskal-Wallis tests according to the qualitative or quantitative status of the variable, and multinomial logistic regressions were performed after adjusting for energy intake in sensitivity analyses.
34224572	4	74	theme	%	734:734	arg1	age					749:751	mean age 43.8 ± 14.7	744:763	mean age 43.8 ± 14.7 y	744:765	METHODS This cross-sectional study described FODMAP intakes in 109,362 volunteers (78.0% female, mean age 43.8 ± 14.7 y) from the French NutriNet-Santé cohort, using an ad hoc FODMAP composition table.
34224572	4	74	theme	%	734:734	arg1	female					736:741	78.0% female	730:741	78.0% female	730:741	METHODS This cross-sectional study described FODMAP intakes in 109,362 volunteers (78.0% female, mean age 43.8 ± 14.7 y) from the French NutriNet-Santé cohort, using an ad hoc FODMAP composition table.
34224572	10	75	theme	FODMAPs	1753:1759	arg1	intake					1743:1748	The highest intake	1731:1748	The highest intake of FODMAPs	1731:1759	The highest intake of FODMAPs was represented by lactose followed by excess fructose, fructans, polyols, and galacto-oligo-saccharides.
34224572	4	76	from	cohort	799:804	arg1	volunteers					718:727	109,362 volunteers	710:727	109,362 volunteers (78.0% female, mean age 43.8 ± 14.7 y) from the French NutriNet-Santé cohort	710:804	METHODS This cross-sectional study described FODMAP intakes in 109,362 volunteers (78.0% female, mean age 43.8 ± 14.7 y) from the French NutriNet-Santé cohort, using an ad hoc FODMAP composition table.
34224572	8	77	with	Participants	1380:1391	arg1	intakes					1405:1411	FODMAP intakes	1398:1411	FODMAP intakes <9 g/d	1398:1418	Participants with FODMAP intakes <9 g/d were more likely to have lower caloric intakes (Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d), to be smokers, to have lower incomes, and to have lower levels of physical activity.
34224572	4	78	dep	volunteers	718:727	arg1	age					749:751	mean age 43.8 ± 14.7	744:763	mean age 43.8 ± 14.7 y	744:765	METHODS This cross-sectional study described FODMAP intakes in 109,362 volunteers (78.0% female, mean age 43.8 ± 14.7 y) from the French NutriNet-Santé cohort, using an ad hoc FODMAP composition table.
34224572	4	78	dep	volunteers	718:727	arg1	female					736:741	78.0% female	730:741	78.0% female	730:741	METHODS This cross-sectional study described FODMAP intakes in 109,362 volunteers (78.0% female, mean age 43.8 ± 14.7 y) from the French NutriNet-Santé cohort, using an ad hoc FODMAP composition table.
34224572	9	79	theme	total	1710:1714	arg1	intake					1723:1728	total energy intake	1710:1728	total energy intake	1710:1728	Total FODMAPs accounted for a mean intake of 18.9 ± 9.5 g/d, which was 3.7 ± 2.0% of total energy intake.
34224572	8	80	theme	FODMAP	1515:1520	arg1	intakes					1522:1528	FODMAP intakes	1515:1528	FODMAP intakes	1515:1528	Participants with FODMAP intakes <9 g/d were more likely to have lower caloric intakes (Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d), to be smokers, to have lower incomes, and to have lower levels of physical activity.
34224572	7	81	dep	RESULTS	1226:1232	arg1	observed					1237:1244	observed	1237:1244	observed a mean intake of 18.9 ± 9.5 g/d FODMAPs in this French cohort	1237:1306	RESULTS We observed a mean intake of 18.9 ± 9.5 g/d FODMAPs in this French cohort, and 11.7% of participants had intakes <9 g/d (i.e., low-FODMAP diets).
34224572	5	82	theme	qualitative	996:1006	arg1	status					1024:1029	the qualitative or quantitative status	992:1029	the qualitative or quantitative status of the variable	992:1045	Associations between FODMAP intakes and sociodemographic characteristics were investigated using χ2 tests or Kruskal-Wallis tests according to the qualitative or quantitative status of the variable, and multinomial logistic regressions were performed after adjusting for energy intake in sensitivity analyses.
34224572	9	83	theme	intake	1723:1728	arg1	%					1705:1705	3.7 ± 2.0%	1696:1705	3.7 ± 2.0% of total energy intake	1696:1728	Total FODMAPs accounted for a mean intake of 18.9 ± 9.5 g/d, which was 3.7 ± 2.0% of total energy intake.
34224572	9	83	theme	intake	1723:1728	arg1	intake					1660:1665	a mean intake	1653:1665	a mean intake	1653:1665	Total FODMAPs accounted for a mean intake of 18.9 ± 9.5 g/d, which was 3.7 ± 2.0% of total energy intake.
34224572	9	83	theme	intake	1723:1728	arg1	intake					1723:1728	total energy intake	1710:1728	total energy intake	1710:1728	Total FODMAPs accounted for a mean intake of 18.9 ± 9.5 g/d, which was 3.7 ± 2.0% of total energy intake.
34224572	9	84	theme	Total	1625:1629	arg1	FODMAPs					1631:1637	Total FODMAPs	1625:1637	Total FODMAPs	1625:1637	Total FODMAPs accounted for a mean intake of 18.9 ± 9.5 g/d, which was 3.7 ± 2.0% of total energy intake.
34224572	1	85	theme	Fermentable	96:106	arg1	oligosaccharides					108:123	BACKGROUND Fermentable oligosaccharides	85:123	BACKGROUND Fermentable oligosaccharides	85:123	BACKGROUND Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) are increasingly studied because they are suspected unfavorably to impact health (irritable bowel syndrome in particular).
34224572	1	85	theme	Fermentable	96:106	arg1	FODMAPs					171:177	FODMAPs	171:177	FODMAPs	171:177	BACKGROUND Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) are increasingly studied because they are suspected unfavorably to impact health (irritable bowel syndrome in particular).
34224572	7	86	theme	intakes	1339:1345	arg1	g/d					1350:1352	intakes <9 g/d	1339:1352	intakes <9 g/d	1339:1352	RESULTS We observed a mean intake of 18.9 ± 9.5 g/d FODMAPs in this French cohort, and 11.7% of participants had intakes <9 g/d (i.e., low-FODMAP diets).
34224572	5	87	from	intake	1127:1132	arg1	analyses					1149:1156	sensitivity analyses	1137:1156	sensitivity analyses	1137:1156	Associations between FODMAP intakes and sociodemographic characteristics were investigated using χ2 tests or Kruskal-Wallis tests according to the qualitative or quantitative status of the variable, and multinomial logistic regressions were performed after adjusting for energy intake in sensitivity analyses.
34224572	8	88	dep	=	1470:1470	arg1	kcal/d					1476:1481	383 kcal/d	1472:1481	383 kcal/d compared with participants with FODMAP intakes	1472:1528	Participants with FODMAP intakes <9 g/d were more likely to have lower caloric intakes (Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d), to be smokers, to have lower incomes, and to have lower levels of physical activity.
34224572	7	89	theme	participants	1322:1333	arg1	participants					1322:1333	participants	1322:1333	participants	1322:1333	RESULTS We observed a mean intake of 18.9 ± 9.5 g/d FODMAPs in this French cohort, and 11.7% of participants had intakes <9 g/d (i.e., low-FODMAP diets).
34224572	7	89	theme	participants	1322:1333	arg1	%					1317:1317	11.7%	1313:1317	11.7% of participants	1313:1333	RESULTS We observed a mean intake of 18.9 ± 9.5 g/d FODMAPs in this French cohort, and 11.7% of participants had intakes <9 g/d (i.e., low-FODMAP diets).
34224572	11	90	theme	FODMAP	1879:1884	arg1	consumption					1886:1896	CONCLUSIONS FODMAP consumption	1867:1896	CONCLUSIONS FODMAP consumption by a large sample of adults from the general population	1867:1952	CONCLUSIONS FODMAP consumption by a large sample of adults from the general population is ∼19 g/d, with half of the population having a FODMAP intake >16 g/d.
34224572	5	91	theme	variable	1038:1045	arg1	status					1024:1029	the qualitative or quantitative status	992:1029	the qualitative or quantitative status of the variable	992:1045	Associations between FODMAP intakes and sociodemographic characteristics were investigated using χ2 tests or Kruskal-Wallis tests according to the qualitative or quantitative status of the variable, and multinomial logistic regressions were performed after adjusting for energy intake in sensitivity analyses.
34224572	8	92	theme	FODMAP	1398:1403	arg1	intakes					1405:1411	FODMAP intakes	1398:1411	FODMAP intakes <9 g/d	1398:1418	Participants with FODMAP intakes <9 g/d were more likely to have lower caloric intakes (Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d), to be smokers, to have lower incomes, and to have lower levels of physical activity.
34224572	0	93	theme	Population-Based	45:60	arg1	Cohort					77:82	the French Population-Based NutriNet-Santé Cohort	34:82	the French Population-Based NutriNet-Santé Cohort	34:82	FODMAP Consumption by Adults from the French Population-Based NutriNet-Santé Cohort.
34224572	4	94	theme	ad	816:817	arg1	table					842:846	an ad hoc FODMAP composition table	813:846	an ad hoc FODMAP composition table	813:846	METHODS This cross-sectional study described FODMAP intakes in 109,362 volunteers (78.0% female, mean age 43.8 ± 14.7 y) from the French NutriNet-Santé cohort, using an ad hoc FODMAP composition table.
34224572	5	95	theme	quantitative	1011:1022	arg1	status					1024:1029	the qualitative or quantitative status	992:1029	the qualitative or quantitative status of the variable	992:1045	Associations between FODMAP intakes and sociodemographic characteristics were investigated using χ2 tests or Kruskal-Wallis tests according to the qualitative or quantitative status of the variable, and multinomial logistic regressions were performed after adjusting for energy intake in sensitivity analyses.
34224572	11	96	theme	large	1903:1907	arg1	sample					1909:1914	a large sample	1901:1914	a large sample of adults from the general population	1901:1952	CONCLUSIONS FODMAP consumption by a large sample of adults from the general population is ∼19 g/d, with half of the population having a FODMAP intake >16 g/d.
34224572	6	97	theme	Eligible	1159:1166	arg1	participants					1168:1179	Eligible participants	1159:1179	Eligible participants	1159:1179	Eligible participants had completed ≥3 detailed 24-h food records.
34224572	8	98	theme	lower	1563:1567	arg1	incomes					1569:1575	lower incomes	1563:1575	lower incomes	1563:1575	Participants with FODMAP intakes <9 g/d were more likely to have lower caloric intakes (Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d), to be smokers, to have lower incomes, and to have lower levels of physical activity.
34224572	4	99	theme	French	777:782	arg1	cohort					799:804	the French NutriNet-Santé cohort	773:804	the French NutriNet-Santé cohort	773:804	METHODS This cross-sectional study described FODMAP intakes in 109,362 volunteers (78.0% female, mean age 43.8 ± 14.7 y) from the French NutriNet-Santé cohort, using an ad hoc FODMAP composition table.
34224572	8	100	theme	activity	1615:1622	arg1	levels					1596:1601	lower levels	1590:1601	lower levels of physical activity	1590:1622	Participants with FODMAP intakes <9 g/d were more likely to have lower caloric intakes (Δ = 383 kcal/d compared with participants with FODMAP intakes ≥16 g/d), to be smokers, to have lower incomes, and to have lower levels of physical activity.
34224572	1	101	theme	impact	247:252	arg1	health					254:259	impact health	247:259	impact health (irritable bowel syndrome in particular)	247:300	BACKGROUND Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) are increasingly studied because they are suspected unfavorably to impact health (irritable bowel syndrome in particular).
34717634	0	0	theme	vascular	94:101	arg1	reconstruction					103:116	vascular reconstruction	94:116	vascular reconstruction	94:116	In vivo performance of electrospun tubular hyaluronic acid/collagen nanofibrous scaffolds for vascular reconstruction in the rabbit model.
34717634	4	1	theme	cells	741:745	arg1	proliferation					703:715	proliferation	703:715	proliferation	703:715	The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood.
34717634	4	1	theme	cells	741:745	arg1	adhesion					690:697	adhesion	690:697	adhesion	690:697	The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood.
34717634	1	2	theme	challenges	155:164	arg1	One					139:141	One	139:141	One	139:141	One of the main challenges of tissue-engineered vascular prostheses is restenosis due to intimal hyperplasia.
34717634	1	2	theme	challenges	155:164	arg1	challenges					155:164	the main challenges	146:164	the main challenges of tissue-engineered vascular prostheses	146:205	One of the main challenges of tissue-engineered vascular prostheses is restenosis due to intimal hyperplasia.
34717634	5	3	theme	luminal	968:974	arg1	surface					976:982	the luminal surface	964:982	the luminal surface	964:982	Carotid artery replacement experiment from 6-week rabbits showed that the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface could maintain vascular patency.
34717634	4	4	theme	biophysical	621:631	arg1	signals					649:655	well-controlled biophysical and biochemical signals	605:655	well-controlled biophysical and biochemical signals	605:655	The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood.
34717634	6	5	theme	good	1065:1068	arg1	integrity					1081:1089	good structural integrity	1065:1089	good structural integrity	1065:1089	At retrieval, the composite scaffold maintained good structural integrity and had comparable mechanical strength as the native artery.
34717634	6	6	theme	composite	1035:1043	arg1	scaffold					1045:1052	the composite scaffold	1031:1052	the composite scaffold	1031:1052	At retrieval, the composite scaffold maintained good structural integrity and had comparable mechanical strength as the native artery.
34717634	5	7	from	rabbits	863:869	arg1	experiment					840:849	Carotid artery replacement experiment	813:849	Carotid artery replacement experiment from 6-week rabbits	813:869	Carotid artery replacement experiment from 6-week rabbits showed that the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface could maintain vascular patency.
34717634	6	8	contain	had	1095:1097	arg2	strength					1121:1128	comparable mechanical strength	1099:1128	comparable mechanical strength	1099:1128	At retrieval, the composite scaffold maintained good structural integrity and had comparable mechanical strength as the native artery.
34717634	6	8	contain	had	1095:1097	arg2	artery					1144:1149	the native artery	1133:1149	the native artery	1133:1149	At retrieval, the composite scaffold maintained good structural integrity and had comparable mechanical strength as the native artery.
34717634	6	8	contain	had	1095:1097	arg1	scaffold					1045:1052	the composite scaffold	1031:1052	the composite scaffold	1031:1052	At retrieval, the composite scaffold maintained good structural integrity and had comparable mechanical strength as the native artery.
34717634	2	9	theme	physiological	355:367	arg1	conditions					369:378	physiological conditions	355:378	physiological conditions	355:378	The aim of this study is to develop a material for scaffolds able to support cell growth while tolerating physiological conditions and maintaining the patency of carotid artery model.
34717634	3	10	theme	composite	498:506	arg1	scaffolds					508:516	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds	433:516	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds	433:516	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds were prepared by sequential electrospinning method.
34717634	0	11	theme	rabbit	125:130	arg1	model					132:136	the rabbit model	121:136	the rabbit model	121:136	In vivo performance of electrospun tubular hyaluronic acid/collagen nanofibrous scaffolds for vascular reconstruction in the rabbit model.
34717634	4	12	dep	has	601:603	arg1	providing					658:666	providing	658:666	providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs)	658:751	The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood.
34717634	4	12	dep	has	601:603	arg1	resisting					758:766	resisting	758:766	resisting to platelets adhesion when exposed to blood	758:810	The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood.
34717634	7	13	theme	electrospun	1179:1189	arg1	scaffolds					1191:1199	electrospun scaffolds	1179:1199	electrospun scaffolds combined with cells	1179:1219	This study indicating that electrospun scaffolds combined with cells may become an alternative to prosthetic grafts for vascular reconstruction.
34717634	3	14	theme	collagen	477:484	arg1	scaffolds					508:516	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds	433:516	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds	433:516	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds were prepared by sequential electrospinning method.
34717634	3	15	theme	Tubular	433:439	arg1	acid					452:455	Tubular hyaluronic acid	433:455	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds	433:516	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds were prepared by sequential electrospinning method.
34717634	3	15	theme	Tubular	433:439	arg1	HA					458:459	HA	458:459	HA	458:459	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds were prepared by sequential electrospinning method.
34717634	1	16	theme	tissue-engineered	169:185	arg1	prostheses					196:205	tissue-engineered vascular prostheses	169:205	tissue-engineered vascular prostheses	169:205	One of the main challenges of tissue-engineered vascular prostheses is restenosis due to intimal hyperplasia.
34717634	4	17	theme	biochemical	637:647	arg1	signals					649:655	well-controlled biophysical and biochemical signals	605:655	well-controlled biophysical and biochemical signals	605:655	The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood.
34717634	5	18	theme	Carotid	813:819	arg1	experiment					840:849	Carotid artery replacement experiment	813:849	Carotid artery replacement experiment from 6-week rabbits	813:869	Carotid artery replacement experiment from 6-week rabbits showed that the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface could maintain vascular patency.
34717634	5	19	theme	6-week	856:861	arg1	rabbits					863:869	6-week rabbits	856:869	6-week rabbits	856:869	Carotid artery replacement experiment from 6-week rabbits showed that the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface could maintain vascular patency.
34717634	1	20	theme	vascular	187:194	arg1	prostheses					196:205	tissue-engineered vascular prostheses	169:205	tissue-engineered vascular prostheses	169:205	One of the main challenges of tissue-engineered vascular prostheses is restenosis due to intimal hyperplasia.
34717634	4	21	contain	has	601:603	arg1	scaffold					592:599	The tubular composite scaffold	570:599	The tubular composite scaffold	570:599	The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood.
34717634	4	21	contain	has	601:603	arg2	signals					649:655	well-controlled biophysical and biochemical signals	605:655	well-controlled biophysical and biochemical signals	605:655	The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood.
34717634	1	22	theme	prostheses	196:205	arg1	challenges					155:164	the main challenges	146:164	the main challenges of tissue-engineered vascular prostheses	146:205	One of the main challenges of tissue-engineered vascular prostheses is restenosis due to intimal hyperplasia.
34717634	0	23	theme	In	0:1	arg1	performance					8:18	In vivo performance	0:18	In vivo performance of electrospun tubular hyaluronic acid/collagen nanofibrous	0:78	In vivo performance of electrospun tubular hyaluronic acid/collagen nanofibrous scaffolds for vascular reconstruction in the rabbit model.
34717634	5	24	theme	composite	911:919	arg1	grafts					930:935	the HA/collagen nanofibrous composite scaffold grafts	883:935	the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface	883:982	Carotid artery replacement experiment from 6-week rabbits showed that the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface could maintain vascular patency.
34717634	2	25	theme	able	310:313	arg1	scaffolds					300:308	scaffolds	300:308	scaffolds able to support cell growth while tolerating physiological conditions and maintaining the patency of carotid artery model	300:430	The aim of this study is to develop a material for scaffolds able to support cell growth while tolerating physiological conditions and maintaining the patency of carotid artery model.
34717634	3	26	theme	hyaluronic	441:450	arg1	acid					452:455	Tubular hyaluronic acid	433:455	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds	433:516	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds were prepared by sequential electrospinning method.
34717634	3	26	theme	hyaluronic	441:450	arg1	HA					458:459	HA	458:459	HA	458:459	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds were prepared by sequential electrospinning method.
34717634	7	27	theme	prosthetic	1250:1259	arg1	grafts					1261:1266	prosthetic grafts	1250:1266	prosthetic grafts	1250:1266	This study indicating that electrospun scaffolds combined with cells may become an alternative to prosthetic grafts for vascular reconstruction.
34717634	4	28	theme	composite	582:590	arg1	scaffold					592:599	The tubular composite scaffold	570:599	The tubular composite scaffold	570:599	The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood.
34717634	5	29	theme	vascular	999:1006	arg1	patency					1008:1014	vascular patency	999:1014	vascular patency	999:1014	Carotid artery replacement experiment from 6-week rabbits showed that the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface could maintain vascular patency.
34717634	5	30	theme	replacement	828:838	arg1	experiment					840:849	Carotid artery replacement experiment	813:849	Carotid artery replacement experiment from 6-week rabbits	813:869	Carotid artery replacement experiment from 6-week rabbits showed that the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface could maintain vascular patency.
34717634	3	31	theme	-functionalized	461:475	arg1	scaffolds					508:516	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds	433:516	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds	433:516	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds were prepared by sequential electrospinning method.
34717634	4	32	theme	well-controlled	605:619	arg1	signals					649:655	well-controlled biophysical and biochemical signals	605:655	well-controlled biophysical and biochemical signals	605:655	The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood.
34717634	2	33	theme	model	426:430	arg1	patency					400:406	the patency	396:406	the patency of carotid artery model	396:430	The aim of this study is to develop a material for scaffolds able to support cell growth while tolerating physiological conditions and maintaining the patency of carotid artery model.
34717634	0	34	theme	tubular	35:41	arg1	nanofibrous					68:78	electrospun tubular hyaluronic acid/collagen nanofibrous	23:78	electrospun tubular hyaluronic acid/collagen nanofibrous	23:78	In vivo performance of electrospun tubular hyaluronic acid/collagen nanofibrous scaffolds for vascular reconstruction in the rabbit model.
34717634	4	35	theme	vascular	720:727	arg1	ECs					748:750	ECs	748:750	ECs	748:750	The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood.
34717634	4	35	theme	vascular	720:727	arg1	cells					741:745	vascular endothelial cells	720:745	vascular endothelial cells (ECs)	720:751	The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood.
34717634	5	36	theme	HA/collagen	887:897	arg1	grafts					930:935	the HA/collagen nanofibrous composite scaffold grafts	883:935	the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface	883:982	Carotid artery replacement experiment from 6-week rabbits showed that the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface could maintain vascular patency.
34717634	4	37	dep	adhesion	690:697	arg1	the					686:688	the	686:688	the	686:688	The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood.
34717634	2	38	theme	artery	419:424	arg1	model					426:430	carotid artery model	411:430	carotid artery model	411:430	The aim of this study is to develop a material for scaffolds able to support cell growth while tolerating physiological conditions and maintaining the patency of carotid artery model.
34717634	0	39	theme	electrospun	23:33	arg1	nanofibrous					68:78	electrospun tubular hyaluronic acid/collagen nanofibrous	23:78	electrospun tubular hyaluronic acid/collagen nanofibrous	23:78	In vivo performance of electrospun tubular hyaluronic acid/collagen nanofibrous scaffolds for vascular reconstruction in the rabbit model.
34717634	5	40	theme	artery	821:826	arg1	experiment					840:849	Carotid artery replacement experiment	813:849	Carotid artery replacement experiment from 6-week rabbits	813:869	Carotid artery replacement experiment from 6-week rabbits showed that the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface could maintain vascular patency.
34717634	6	41	theme	structural	1070:1079	arg1	integrity					1081:1089	good structural integrity	1065:1089	good structural integrity	1065:1089	At retrieval, the composite scaffold maintained good structural integrity and had comparable mechanical strength as the native artery.
34717634	5	42	theme	nanofibrous	899:909	arg1	grafts					930:935	the HA/collagen nanofibrous composite scaffold grafts	883:935	the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface	883:982	Carotid artery replacement experiment from 6-week rabbits showed that the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface could maintain vascular patency.
34717634	1	43	theme	intimal	228:234	arg1	hyperplasia					236:246	intimal hyperplasia	228:246	intimal hyperplasia	228:246	One of the main challenges of tissue-engineered vascular prostheses is restenosis due to intimal hyperplasia.
34717634	0	44	theme	acid/collagen	54:66	arg1	nanofibrous					68:78	electrospun tubular hyaluronic acid/collagen nanofibrous	23:78	electrospun tubular hyaluronic acid/collagen nanofibrous	23:78	In vivo performance of electrospun tubular hyaluronic acid/collagen nanofibrous scaffolds for vascular reconstruction in the rabbit model.
34717634	5	45	with	grafts	930:935	arg1	endothelialization					942:959	endothelialization	942:959	endothelialization	942:959	Carotid artery replacement experiment from 6-week rabbits showed that the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface could maintain vascular patency.
34717634	3	46	theme	acid	452:455	arg1	scaffolds					508:516	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds	433:516	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds	433:516	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds were prepared by sequential electrospinning method.
34717634	0	47	theme	hyaluronic	43:52	arg1	nanofibrous					68:78	electrospun tubular hyaluronic acid/collagen nanofibrous	23:78	electrospun tubular hyaluronic acid/collagen nanofibrous	23:78	In vivo performance of electrospun tubular hyaluronic acid/collagen nanofibrous scaffolds for vascular reconstruction in the rabbit model.
34717634	4	48	theme	tubular	574:580	arg1	scaffold					592:599	The tubular composite scaffold	570:599	The tubular composite scaffold	570:599	The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood.
34717634	4	49	dep	platelets	771:779	arg1	adhesion					781:788	adhesion	781:788	adhesion	781:788	The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood.
34717634	6	50	theme	comparable	1099:1108	arg1	strength					1121:1128	comparable mechanical strength	1099:1128	comparable mechanical strength	1099:1128	At retrieval, the composite scaffold maintained good structural integrity and had comparable mechanical strength as the native artery.
34717634	6	50	theme	comparable	1099:1108	arg1	artery					1144:1149	the native artery	1133:1149	the native artery	1133:1149	At retrieval, the composite scaffold maintained good structural integrity and had comparable mechanical strength as the native artery.
34717634	4	51	theme	endothelial	729:739	arg1	ECs					748:750	ECs	748:750	ECs	748:750	The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood.
34717634	4	51	theme	endothelial	729:739	arg1	cells					741:745	vascular endothelial cells	720:745	vascular endothelial cells (ECs)	720:751	The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood.
34717634	2	52	theme	cell	326:329	arg1	growth					331:336	cell growth	326:336	cell growth	326:336	The aim of this study is to develop a material for scaffolds able to support cell growth while tolerating physiological conditions and maintaining the patency of carotid artery model.
34717634	6	53	theme	mechanical	1110:1119	arg1	strength					1121:1128	comparable mechanical strength	1099:1128	comparable mechanical strength	1099:1128	At retrieval, the composite scaffold maintained good structural integrity and had comparable mechanical strength as the native artery.
34717634	6	53	theme	mechanical	1110:1119	arg1	artery					1144:1149	the native artery	1133:1149	the native artery	1133:1149	At retrieval, the composite scaffold maintained good structural integrity and had comparable mechanical strength as the native artery.
34717634	2	54	theme	study	265:269	arg1	aim					253:255	The aim	249:255	The aim of this study	249:269	The aim of this study is to develop a material for scaffolds able to support cell growth while tolerating physiological conditions and maintaining the patency of carotid artery model.
34717634	5	55	from	grafts	930:935	arg1	surface					976:982	the luminal surface	964:982	the luminal surface	964:982	Carotid artery replacement experiment from 6-week rabbits showed that the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface could maintain vascular patency.
34717634	3	56	theme	electrospinning	546:560	arg1	method					562:567	sequential electrospinning method	535:567	sequential electrospinning method	535:567	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds were prepared by sequential electrospinning method.
34717634	3	57	theme	nanofibrous	486:496	arg1	scaffolds					508:516	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds	433:516	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds	433:516	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds were prepared by sequential electrospinning method.
34717634	2	58	theme	carotid	411:417	arg1	model					426:430	carotid artery model	411:430	carotid artery model	411:430	The aim of this study is to develop a material for scaffolds able to support cell growth while tolerating physiological conditions and maintaining the patency of carotid artery model.
34717634	6	59	theme	native	1137:1142	arg1	strength					1121:1128	comparable mechanical strength	1099:1128	comparable mechanical strength	1099:1128	At retrieval, the composite scaffold maintained good structural integrity and had comparable mechanical strength as the native artery.
34717634	6	59	theme	native	1137:1142	arg1	artery					1144:1149	the native artery	1133:1149	the native artery	1133:1149	At retrieval, the composite scaffold maintained good structural integrity and had comparable mechanical strength as the native artery.
34717634	3	60	theme	sequential	535:544	arg1	method					562:567	sequential electrospinning method	535:567	sequential electrospinning method	535:567	Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds were prepared by sequential electrospinning method.
34717634	5	61	theme	scaffold	921:928	arg1	grafts					930:935	the HA/collagen nanofibrous composite scaffold grafts	883:935	the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface	883:982	Carotid artery replacement experiment from 6-week rabbits showed that the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface could maintain vascular patency.
34717634	4	62	theme	good	670:673	arg1	matrix					675:680	a good matrix	668:680	a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs)	668:751	The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood.
34717634	0	63	theme	nanofibrous	68:78	arg1	performance					8:18	In vivo performance	0:18	In vivo performance of electrospun tubular hyaluronic acid/collagen nanofibrous	0:78	In vivo performance of electrospun tubular hyaluronic acid/collagen nanofibrous scaffolds for vascular reconstruction in the rabbit model.
34717634	0	64	dep	In	0:1	arg1	vivo					3:6	vivo	3:6	vivo	3:6	In vivo performance of electrospun tubular hyaluronic acid/collagen nanofibrous scaffolds for vascular reconstruction in the rabbit model.
34717634	1	65	theme	main	150:153	arg1	challenges					155:164	the main challenges	146:164	the main challenges of tissue-engineered vascular prostheses	146:205	One of the main challenges of tissue-engineered vascular prostheses is restenosis due to intimal hyperplasia.
34717634	7	66	theme	vascular	1272:1279	arg1	reconstruction					1281:1294	vascular reconstruction	1272:1294	vascular reconstruction	1272:1294	This study indicating that electrospun scaffolds combined with cells may become an alternative to prosthetic grafts for vascular reconstruction.
34464860	4	0	theme	good	561:564	arg1	shaping					566:572	The good shaping and mechanical properties	557:598	shaping	566:572	The good shaping and mechanical properties of polydimethylsiloxane provide a specific recognition site suitable for Staphylococcus aureus.
34464860	5	1	theme	two-component	788:800	arg1	film					812:815	a two-component composite film	786:815	a two-component composite film	786:815	For the first time, chitosan derivatives is combined with polydimethylsiloxane to prepare a two-component composite film, which possesses a remarkable absorption performance of Staphylococcus aureus using the natural excellent absorption property of chitosan.
34464860	5	2	theme	absorption	847:856	arg1	performance					858:868	a remarkable absorption performance	834:868	a remarkable absorption performance of Staphylococcus aureus using the natural excellent absorption property of chitosan	834:953	For the first time, chitosan derivatives is combined with polydimethylsiloxane to prepare a two-component composite film, which possesses a remarkable absorption performance of Staphylococcus aureus using the natural excellent absorption property of chitosan.
34464860	0	3	theme	composite	106:114	arg1	film					116:119	N-Succinyl-Chitosan-dopped bacteria-imprinted composite film and AIE fluorescence sensor	60:147	film	116:119	A concise detection strategy of Staphylococcus aureus using N-Succinyl-Chitosan-dopped bacteria-imprinted composite film and AIE fluorescence sensor.
34464860	4	4	theme	mechanical	578:587	arg1	properties					589:598	The good shaping and mechanical properties	557:598	properties	589:598	The good shaping and mechanical properties of polydimethylsiloxane provide a specific recognition site suitable for Staphylococcus aureus.
34464860	1	5	theme	major	186:190	arg1	pathogens					202:210	the major foodborne pathogens	182:210	the major foodborne pathogens	182:210	Staphylococcus aureus is one of the major foodborne pathogens.
34464860	4	6	theme	suitable	660:667	arg1	site					655:658	a specific recognition site	632:658	a specific recognition site suitable for Staphylococcus aureus	632:693	The good shaping and mechanical properties of polydimethylsiloxane provide a specific recognition site suitable for Staphylococcus aureus.
34464860	0	7	theme	AIE	125:127	arg1	sensor					142:147	N-Succinyl-Chitosan-dopped bacteria-imprinted composite film and AIE fluorescence sensor	60:147	sensor	142:147	A concise detection strategy of Staphylococcus aureus using N-Succinyl-Chitosan-dopped bacteria-imprinted composite film and AIE fluorescence sensor.
34464860	7	8	with	separators	1241:1250	arg1	performance					1266:1276	superior performance	1257:1276	superior performance	1257:1276	To conclude, this study provides new possibilities for the manufacture of highly efficient bacterial separators with superior performance and facilitates the application of unlabeled nanoparticles in quantitative analysis.
34464860	5	9	theme	aureus	888:893	arg1	performance					858:868	a remarkable absorption performance	834:868	a remarkable absorption performance of Staphylococcus aureus using the natural excellent absorption property of chitosan	834:953	For the first time, chitosan derivatives is combined with polydimethylsiloxane to prepare a two-component composite film, which possesses a remarkable absorption performance of Staphylococcus aureus using the natural excellent absorption property of chitosan.
34464860	5	10	theme	first	704:708	arg1	time					710:713	the first time	700:713	the first time	700:713	For the first time, chitosan derivatives is combined with polydimethylsiloxane to prepare a two-component composite film, which possesses a remarkable absorption performance of Staphylococcus aureus using the natural excellent absorption property of chitosan.
34464860	1	11	theme	foodborne	192:200	arg1	pathogens					202:210	the major foodborne pathogens	182:210	the major foodborne pathogens	182:210	Staphylococcus aureus is one of the major foodborne pathogens.
34464860	0	12	theme	fluorescence	129:140	arg1	sensor					142:147	N-Succinyl-Chitosan-dopped bacteria-imprinted composite film and AIE fluorescence sensor	60:147	sensor	142:147	A concise detection strategy of Staphylococcus aureus using N-Succinyl-Chitosan-dopped bacteria-imprinted composite film and AIE fluorescence sensor.
34464860	6	13	theme	-disulfide	996:1005	arg1	nanoparticles					1007:1019	The positive charged AIE-featuring Au(I)-disulfide nanoparticles	956:1019	The positive charged AIE-featuring Au(I)-disulfide nanoparticles	956:1019	The positive charged AIE-featuring Au(I)-disulfide nanoparticles realized the quantitative characterization of Staphylococcus aureus without cooperation with bio-recognition elements.
34464860	6	14	theme	AIE-featuring	977:989	arg1	Au					991:992	The positive charged AIE-featuring Au	956:992	The positive charged AIE-featuring Au(I)-disulfide nanoparticles	956:1019	The positive charged AIE-featuring Au(I)-disulfide nanoparticles realized the quantitative characterization of Staphylococcus aureus without cooperation with bio-recognition elements.
34464860	6	14	theme	AIE-featuring	977:989	arg1	I					994:994	I	994:994	I	994:994	The positive charged AIE-featuring Au(I)-disulfide nanoparticles realized the quantitative characterization of Staphylococcus aureus without cooperation with bio-recognition elements.
34464860	1	15	theme	pathogens	202:210	arg1	pathogens					202:210	the major foodborne pathogens	182:210	the major foodborne pathogens	182:210	Staphylococcus aureus is one of the major foodborne pathogens.
34464860	1	15	theme	pathogens	202:210	arg1	one					175:177	one	175:177	one	175:177	Staphylococcus aureus is one of the major foodborne pathogens.
34464860	0	16	theme	concise	2:8	arg1	strategy					20:27	A concise detection strategy	0:27	A concise detection strategy of Staphylococcus aureus	0:52	A concise detection strategy of Staphylococcus aureus using N-Succinyl-Chitosan-dopped bacteria-imprinted composite film and AIE fluorescence sensor.
34464860	5	17	contain	possesses	824:832	arg1	film					812:815	a two-component composite film	786:815	a two-component composite film	786:815	For the first time, chitosan derivatives is combined with polydimethylsiloxane to prepare a two-component composite film, which possesses a remarkable absorption performance of Staphylococcus aureus using the natural excellent absorption property of chitosan.
34464860	5	17	contain	possesses	824:832	arg2	performance					858:868	a remarkable absorption performance	834:868	a remarkable absorption performance of Staphylococcus aureus using the natural excellent absorption property of chitosan	834:953	For the first time, chitosan derivatives is combined with polydimethylsiloxane to prepare a two-component composite film, which possesses a remarkable absorption performance of Staphylococcus aureus using the natural excellent absorption property of chitosan.
34464860	5	18	theme	chitosan	716:723	arg1	derivatives					725:735	chitosan derivatives	716:735	chitosan derivatives	716:735	For the first time, chitosan derivatives is combined with polydimethylsiloxane to prepare a two-component composite film, which possesses a remarkable absorption performance of Staphylococcus aureus using the natural excellent absorption property of chitosan.
34464860	6	19	theme	quantitative	1034:1045	arg1	characterization					1047:1062	the quantitative characterization	1030:1062	the quantitative characterization of Staphylococcus aureus	1030:1087	The positive charged AIE-featuring Au(I)-disulfide nanoparticles realized the quantitative characterization of Staphylococcus aureus without cooperation with bio-recognition elements.
34464860	4	20	theme	specific	634:641	arg1	site					655:658	a specific recognition site	632:658	a specific recognition site suitable for Staphylococcus aureus	632:693	The good shaping and mechanical properties of polydimethylsiloxane provide a specific recognition site suitable for Staphylococcus aureus.
34464860	7	21	theme	superior	1257:1264	arg1	performance					1266:1276	superior performance	1257:1276	superior performance	1257:1276	To conclude, this study provides new possibilities for the manufacture of highly efficient bacterial separators with superior performance and facilitates the application of unlabeled nanoparticles in quantitative analysis.
34464860	7	22	from	application	1298:1308	arg1	analysis					1353:1360	quantitative analysis	1340:1360	quantitative analysis	1340:1360	To conclude, this study provides new possibilities for the manufacture of highly efficient bacterial separators with superior performance and facilitates the application of unlabeled nanoparticles in quantitative analysis.
34464860	0	23	theme	aureus	47:52	arg1	strategy					20:27	A concise detection strategy	0:27	A concise detection strategy of Staphylococcus aureus	0:52	A concise detection strategy of Staphylococcus aureus using N-Succinyl-Chitosan-dopped bacteria-imprinted composite film and AIE fluorescence sensor.
34464860	5	24	theme	composite	802:810	arg1	film					812:815	a two-component composite film	786:815	a two-component composite film	786:815	For the first time, chitosan derivatives is combined with polydimethylsiloxane to prepare a two-component composite film, which possesses a remarkable absorption performance of Staphylococcus aureus using the natural excellent absorption property of chitosan.
34464860	5	25	theme	natural	905:911	arg1	property					934:941	the natural excellent absorption property	901:941	the natural excellent absorption property of chitosan	901:953	For the first time, chitosan derivatives is combined with polydimethylsiloxane to prepare a two-component composite film, which possesses a remarkable absorption performance of Staphylococcus aureus using the natural excellent absorption property of chitosan.
34464860	2	26	from	samples	285:291	arg1	isolation					237:245	isolation	237:245	isolation	237:245	Efficient detection and isolation of Staphylococcus aureus from complex samples are crucial.
34464860	2	26	from	samples	285:291	arg1	detection					223:231	detection	223:231	detection	223:231	Efficient detection and isolation of Staphylococcus aureus from complex samples are crucial.
34464860	0	27	theme	detection	10:18	arg1	strategy					20:27	A concise detection strategy	0:27	A concise detection strategy of Staphylococcus aureus	0:52	A concise detection strategy of Staphylococcus aureus using N-Succinyl-Chitosan-dopped bacteria-imprinted composite film and AIE fluorescence sensor.
34464860	6	28	theme	bio-recognition	1114:1128	arg1	elements					1130:1137	bio-recognition elements	1114:1137	bio-recognition elements	1114:1137	The positive charged AIE-featuring Au(I)-disulfide nanoparticles realized the quantitative characterization of Staphylococcus aureus without cooperation with bio-recognition elements.
34464860	7	29	theme	separators	1241:1250	arg1	manufacture					1199:1209	the manufacture	1195:1209	the manufacture of highly efficient bacterial separators with superior performance	1195:1276	To conclude, this study provides new possibilities for the manufacture of highly efficient bacterial separators with superior performance and facilitates the application of unlabeled nanoparticles in quantitative analysis.
34464860	2	30	theme	Efficient	213:221	arg1	detection					223:231	detection	223:231	detection	223:231	Efficient detection and isolation of Staphylococcus aureus from complex samples are crucial.
34464860	7	31	theme	quantitative	1340:1351	arg1	analysis					1353:1360	quantitative analysis	1340:1360	quantitative analysis	1340:1360	To conclude, this study provides new possibilities for the manufacture of highly efficient bacterial separators with superior performance and facilitates the application of unlabeled nanoparticles in quantitative analysis.
34464860	3	32	theme	-featuring	525:534	arg1	sensor					549:554	aggregation-induced emission (AIE)-featuring fluorescence sensor	491:554	aggregation-induced emission (AIE)-featuring fluorescence sensor	491:554	Herein, we report a concise strategy to detect of Staphylococcus aureus with high sensitivity and specificity, based on N-Succinyl-Chitosan doping bacteria-imprinted composite film and aggregation-induced emission (AIE)-featuring fluorescence sensor.
34464860	3	33	theme	bacteria-imprinted	453:470	arg1	film					482:485	N-Succinyl-Chitosan doping bacteria-imprinted composite film	426:485	N-Succinyl-Chitosan doping bacteria-imprinted composite film	426:485	Herein, we report a concise strategy to detect of Staphylococcus aureus with high sensitivity and specificity, based on N-Succinyl-Chitosan doping bacteria-imprinted composite film and aggregation-induced emission (AIE)-featuring fluorescence sensor.
34464860	4	34	theme	recognition	643:653	arg1	site					655:658	a specific recognition site	632:658	a specific recognition site suitable for Staphylococcus aureus	632:693	The good shaping and mechanical properties of polydimethylsiloxane provide a specific recognition site suitable for Staphylococcus aureus.
34464860	2	35	theme	complex	277:283	arg1	samples					285:291	complex samples	277:291	complex samples	277:291	Efficient detection and isolation of Staphylococcus aureus from complex samples are crucial.
34464860	7	36	theme	unlabeled	1313:1321	arg1	nanoparticles					1323:1335	unlabeled nanoparticles	1313:1335	unlabeled nanoparticles	1313:1335	To conclude, this study provides new possibilities for the manufacture of highly efficient bacterial separators with superior performance and facilitates the application of unlabeled nanoparticles in quantitative analysis.
34464860	3	37	theme	composite	472:480	arg1	film					482:485	N-Succinyl-Chitosan doping bacteria-imprinted composite film	426:485	N-Succinyl-Chitosan doping bacteria-imprinted composite film	426:485	Herein, we report a concise strategy to detect of Staphylococcus aureus with high sensitivity and specificity, based on N-Succinyl-Chitosan doping bacteria-imprinted composite film and aggregation-induced emission (AIE)-featuring fluorescence sensor.
34464860	7	38	theme	efficient	1221:1229	arg1	separators					1241:1250	highly efficient bacterial separators	1214:1250	highly efficient bacterial separators with superior performance	1214:1276	To conclude, this study provides new possibilities for the manufacture of highly efficient bacterial separators with superior performance and facilitates the application of unlabeled nanoparticles in quantitative analysis.
34464860	6	39	theme	Au	991:992	arg1	nanoparticles					1007:1019	The positive charged AIE-featuring Au(I)-disulfide nanoparticles	956:1019	The positive charged AIE-featuring Au(I)-disulfide nanoparticles	956:1019	The positive charged AIE-featuring Au(I)-disulfide nanoparticles realized the quantitative characterization of Staphylococcus aureus without cooperation with bio-recognition elements.
34464860	7	40	theme	new	1173:1175	arg1	possibilities					1177:1189	new possibilities	1173:1189	new possibilities for the manufacture of highly efficient bacterial separators with superior performance	1173:1276	To conclude, this study provides new possibilities for the manufacture of highly efficient bacterial separators with superior performance and facilitates the application of unlabeled nanoparticles in quantitative analysis.
34464860	3	41	theme	high	383:386	arg1	sensitivity					388:398	high sensitivity	383:398	high sensitivity	383:398	Herein, we report a concise strategy to detect of Staphylococcus aureus with high sensitivity and specificity, based on N-Succinyl-Chitosan doping bacteria-imprinted composite film and aggregation-induced emission (AIE)-featuring fluorescence sensor.
34464860	3	42	theme	N-Succinyl-Chitosan	426:444	arg1	film					482:485	N-Succinyl-Chitosan doping bacteria-imprinted composite film	426:485	N-Succinyl-Chitosan doping bacteria-imprinted composite film	426:485	Herein, we report a concise strategy to detect of Staphylococcus aureus with high sensitivity and specificity, based on N-Succinyl-Chitosan doping bacteria-imprinted composite film and aggregation-induced emission (AIE)-featuring fluorescence sensor.
34464860	5	43	theme	excellent	913:921	arg1	property					934:941	the natural excellent absorption property	901:941	the natural excellent absorption property of chitosan	901:953	For the first time, chitosan derivatives is combined with polydimethylsiloxane to prepare a two-component composite film, which possesses a remarkable absorption performance of Staphylococcus aureus using the natural excellent absorption property of chitosan.
34464860	3	44	theme	aggregation-induced	491:509	arg1	sensor					549:554	aggregation-induced emission (AIE)-featuring fluorescence sensor	491:554	aggregation-induced emission (AIE)-featuring fluorescence sensor	491:554	Herein, we report a concise strategy to detect of Staphylococcus aureus with high sensitivity and specificity, based on N-Succinyl-Chitosan doping bacteria-imprinted composite film and aggregation-induced emission (AIE)-featuring fluorescence sensor.
34464860	2	45	theme	aureus	265:270	arg1	isolation					237:245	isolation	237:245	isolation	237:245	Efficient detection and isolation of Staphylococcus aureus from complex samples are crucial.
34464860	2	45	theme	aureus	265:270	arg1	detection					223:231	detection	223:231	detection	223:231	Efficient detection and isolation of Staphylococcus aureus from complex samples are crucial.
34464860	3	46	theme	doping	446:451	arg1	film					482:485	N-Succinyl-Chitosan doping bacteria-imprinted composite film	426:485	N-Succinyl-Chitosan doping bacteria-imprinted composite film	426:485	Herein, we report a concise strategy to detect of Staphylococcus aureus with high sensitivity and specificity, based on N-Succinyl-Chitosan doping bacteria-imprinted composite film and aggregation-induced emission (AIE)-featuring fluorescence sensor.
34464860	6	47	theme	aureus	1082:1087	arg1	characterization					1047:1062	the quantitative characterization	1030:1062	the quantitative characterization of Staphylococcus aureus	1030:1087	The positive charged AIE-featuring Au(I)-disulfide nanoparticles realized the quantitative characterization of Staphylococcus aureus without cooperation with bio-recognition elements.
34464860	7	48	theme	bacterial	1231:1239	arg1	separators					1241:1250	highly efficient bacterial separators	1214:1250	highly efficient bacterial separators with superior performance	1214:1276	To conclude, this study provides new possibilities for the manufacture of highly efficient bacterial separators with superior performance and facilitates the application of unlabeled nanoparticles in quantitative analysis.
34464860	6	49	theme	charged	969:975	arg1	Au					991:992	The positive charged AIE-featuring Au	956:992	The positive charged AIE-featuring Au(I)-disulfide nanoparticles	956:1019	The positive charged AIE-featuring Au(I)-disulfide nanoparticles realized the quantitative characterization of Staphylococcus aureus without cooperation with bio-recognition elements.
34464860	6	49	theme	charged	969:975	arg1	I					994:994	I	994:994	I	994:994	The positive charged AIE-featuring Au(I)-disulfide nanoparticles realized the quantitative characterization of Staphylococcus aureus without cooperation with bio-recognition elements.
34464860	4	50	theme	polydimethylsiloxane	603:622	arg1	shaping					566:572	The good shaping and mechanical properties	557:598	shaping	566:572	The good shaping and mechanical properties of polydimethylsiloxane provide a specific recognition site suitable for Staphylococcus aureus.
34464860	4	50	theme	polydimethylsiloxane	603:622	arg1	properties					589:598	The good shaping and mechanical properties	557:598	properties	589:598	The good shaping and mechanical properties of polydimethylsiloxane provide a specific recognition site suitable for Staphylococcus aureus.
34464860	6	51	theme	positive	960:967	arg1	Au					991:992	The positive charged AIE-featuring Au	956:992	The positive charged AIE-featuring Au(I)-disulfide nanoparticles	956:1019	The positive charged AIE-featuring Au(I)-disulfide nanoparticles realized the quantitative characterization of Staphylococcus aureus without cooperation with bio-recognition elements.
34464860	6	51	theme	positive	960:967	arg1	I					994:994	I	994:994	I	994:994	The positive charged AIE-featuring Au(I)-disulfide nanoparticles realized the quantitative characterization of Staphylococcus aureus without cooperation with bio-recognition elements.
34464860	7	52	theme	nanoparticles	1323:1335	arg1	application					1298:1308	the application	1294:1308	the application of unlabeled nanoparticles in quantitative analysis	1294:1360	To conclude, this study provides new possibilities for the manufacture of highly efficient bacterial separators with superior performance and facilitates the application of unlabeled nanoparticles in quantitative analysis.
34464860	3	53	theme	fluorescence	536:547	arg1	sensor					549:554	aggregation-induced emission (AIE)-featuring fluorescence sensor	491:554	aggregation-induced emission (AIE)-featuring fluorescence sensor	491:554	Herein, we report a concise strategy to detect of Staphylococcus aureus with high sensitivity and specificity, based on N-Succinyl-Chitosan doping bacteria-imprinted composite film and aggregation-induced emission (AIE)-featuring fluorescence sensor.
34464860	6	54	with	cooperation	1097:1107	arg1	elements					1130:1137	bio-recognition elements	1114:1137	bio-recognition elements	1114:1137	The positive charged AIE-featuring Au(I)-disulfide nanoparticles realized the quantitative characterization of Staphylococcus aureus without cooperation with bio-recognition elements.
34464860	5	55	theme	remarkable	836:845	arg1	performance					858:868	a remarkable absorption performance	834:868	a remarkable absorption performance of Staphylococcus aureus using the natural excellent absorption property of chitosan	834:953	For the first time, chitosan derivatives is combined with polydimethylsiloxane to prepare a two-component composite film, which possesses a remarkable absorption performance of Staphylococcus aureus using the natural excellent absorption property of chitosan.
34464860	3	56	theme	concise	326:332	arg1	strategy					334:341	a concise strategy	324:341	a concise strategy to detect of Staphylococcus aureus with high sensitivity and specificity, based on N-Succinyl-Chitosan doping bacteria-imprinted composite film and aggregation-induced emission (AIE)-featuring fluorescence sensor	324:554	Herein, we report a concise strategy to detect of Staphylococcus aureus with high sensitivity and specificity, based on N-Succinyl-Chitosan doping bacteria-imprinted composite film and aggregation-induced emission (AIE)-featuring fluorescence sensor.
34464860	0	57	theme	bacteria-imprinted	87:104	arg1	film					116:119	N-Succinyl-Chitosan-dopped bacteria-imprinted composite film and AIE fluorescence sensor	60:147	film	116:119	A concise detection strategy of Staphylococcus aureus using N-Succinyl-Chitosan-dopped bacteria-imprinted composite film and AIE fluorescence sensor.
34464860	3	58	theme	AIE	521:523	arg1	sensor					549:554	aggregation-induced emission (AIE)-featuring fluorescence sensor	491:554	aggregation-induced emission (AIE)-featuring fluorescence sensor	491:554	Herein, we report a concise strategy to detect of Staphylococcus aureus with high sensitivity and specificity, based on N-Succinyl-Chitosan doping bacteria-imprinted composite film and aggregation-induced emission (AIE)-featuring fluorescence sensor.
34464860	3	59	theme	emission	511:518	arg1	sensor					549:554	aggregation-induced emission (AIE)-featuring fluorescence sensor	491:554	aggregation-induced emission (AIE)-featuring fluorescence sensor	491:554	Herein, we report a concise strategy to detect of Staphylococcus aureus with high sensitivity and specificity, based on N-Succinyl-Chitosan doping bacteria-imprinted composite film and aggregation-induced emission (AIE)-featuring fluorescence sensor.
34464860	0	60	theme	N-Succinyl-Chitosan-dopped	60:85	arg1	film					116:119	N-Succinyl-Chitosan-dopped bacteria-imprinted composite film and AIE fluorescence sensor	60:147	film	116:119	A concise detection strategy of Staphylococcus aureus using N-Succinyl-Chitosan-dopped bacteria-imprinted composite film and AIE fluorescence sensor.
34464860	5	61	theme	chitosan	946:953	arg1	property					934:941	the natural excellent absorption property	901:941	the natural excellent absorption property of chitosan	901:953	For the first time, chitosan derivatives is combined with polydimethylsiloxane to prepare a two-component composite film, which possesses a remarkable absorption performance of Staphylococcus aureus using the natural excellent absorption property of chitosan.
34464860	5	62	theme	absorption	923:932	arg1	property					934:941	the natural excellent absorption property	901:941	the natural excellent absorption property of chitosan	901:953	For the first time, chitosan derivatives is combined with polydimethylsiloxane to prepare a two-component composite film, which possesses a remarkable absorption performance of Staphylococcus aureus using the natural excellent absorption property of chitosan.
31936588	6	0	theme	carbohydrate	898:909	arg1	determinants					911:922	cross-reactive carbohydrate determinants	883:922	cross-reactive carbohydrate determinants	883:922	The aGal epitopes and cross-reactive carbohydrate determinants (CCD) increases over time after the onset of blood feeding in both male and female A. americanum.
31936588	0	1	from	Determinants	42:53	arg1	N-Glycans					62:70	the N-Glycans	58:70	the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum	58:133	Alpha-Gal and Cross-Reactive Carbohydrate Determinants in the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum.
31936588	7	2	theme	1,3-	1088:1091	arg1	1,6-difucosylations					1097:1115	1,6-difucosylations	1097:1115	1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal	1097:1165	These CCDs include xylosylation of the core mannose, 1,3-mono and 1,3- and 1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal.
31936588	7	2	theme	1,3-	1088:1091	arg1	xylosylation					1041:1052	xylosylation	1041:1052	xylosylation of the core mannose, 1,3-mono and 1,3-	1041:1091	These CCDs include xylosylation of the core mannose, 1,3-mono and 1,3- and 1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal.
31936588	2	3	theme	pathogen	345:352	arg1	transmission					354:365	the pathogen transmission	341:365	the pathogen transmission	341:365	Ticks secrete saliva that contains various bioactive materials to evade the host defense system, and often facilitates the pathogen transmission.
31936588	2	4	contain	contains	248:255	arg2	materials					275:283	various bioactive materials	257:283	various bioactive materials	257:283	Ticks secrete saliva that contains various bioactive materials to evade the host defense system, and often facilitates the pathogen transmission.
31936588	2	4	contain	contains	248:255	arg1	saliva					236:241	saliva	236:241	saliva that contains various bioactive materials to evade the host defense system, and often facilitates the pathogen transmission	236:365	Ticks secrete saliva that contains various bioactive materials to evade the host defense system, and often facilitates the pathogen transmission.
31936588	7	5	theme	1,3-mono	1075:1082	arg1	1,6-difucosylations					1097:1115	1,6-difucosylations	1097:1115	1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal	1097:1165	These CCDs include xylosylation of the core mannose, 1,3-mono and 1,3- and 1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal.
31936588	7	5	theme	1,3-mono	1075:1082	arg1	xylosylation					1041:1052	xylosylation	1041:1052	xylosylation of the core mannose, 1,3-mono and 1,3-	1041:1091	These CCDs include xylosylation of the core mannose, 1,3-mono and 1,3- and 1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal.
31936588	6	6	theme	aGal	865:868	arg1	epitopes					870:877	aGal epitopes	865:877	aGal epitopes	865:877	The aGal epitopes and cross-reactive carbohydrate determinants (CCD) increases over time after the onset of blood feeding in both male and female A. americanum.
31936588	6	6	theme	aGal	865:868	arg1	CCD					925:927	CCD	925:927	CCD	925:927	The aGal epitopes and cross-reactive carbohydrate determinants (CCD) increases over time after the onset of blood feeding in both male and female A. americanum.
31936588	8	7	theme	aGal	1206:1209	arg1	aGal					1232:1235	aGal	1232:1235	aGal	1232:1235	Combinations of both xylosylation and aGal and fucosylation and aGal were also found on the N-glycan structures.
31936588	8	7	theme	aGal	1206:1209	arg1	fucosylation					1215:1226	fucosylation	1215:1226	fucosylation	1215:1226	Combinations of both xylosylation and aGal and fucosylation and aGal were also found on the N-glycan structures.
31936588	8	7	theme	aGal	1206:1209	arg1	Combinations					1168:1179	Combinations	1168:1179	Combinations of both xylosylation and aGal	1168:1209	Combinations of both xylosylation and aGal and fucosylation and aGal were also found on the N-glycan structures.
31936588	7	8	theme	mannose	1066:1072	arg1	1,6-difucosylations					1097:1115	1,6-difucosylations	1097:1115	1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal	1097:1165	These CCDs include xylosylation of the core mannose, 1,3-mono and 1,3- and 1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal.
31936588	7	8	theme	mannose	1066:1072	arg1	xylosylation					1041:1052	xylosylation	1041:1052	xylosylation of the core mannose, 1,3-mono and 1,3-	1041:1091	These CCDs include xylosylation of the core mannose, 1,3-mono and 1,3- and 1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal.
31936588	3	9	theme	glycan	508:513	arg1	moieties					515:522	glycan moieties	508:522	glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal)	508:577	In addition, the Lone star tick saliva is thought to be the sensitizer in red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal).
31936588	4	10	from	structures	650:659	arg1	stages					723:728	three different feeding stages	699:728	three different feeding stages	699:728	To assess N-glycome of Amblyomma americanum, we examined the N-glycan structures in male and female salivary glands at three different feeding stages and in carcasses of partially fed lone star ticks.
31936588	4	10	from	structures	650:659	arg1	glands					689:694	male and female salivary glands	664:694	male and female salivary glands at three different feeding stages	664:728	To assess N-glycome of Amblyomma americanum, we examined the N-glycan structures in male and female salivary glands at three different feeding stages and in carcasses of partially fed lone star ticks.
31936588	4	10	from	structures	650:659	arg1	carcasses					737:745	carcasses	737:745	carcasses of partially fed lone star ticks	737:778	To assess N-glycome of Amblyomma americanum, we examined the N-glycan structures in male and female salivary glands at three different feeding stages and in carcasses of partially fed lone star ticks.
31936588	5	11	gly	N-glycosylation	824:838	arg1	species					852:858	the tick species	843:858	the tick species	843:858	We also surveyed the genes involved in the N-glycosylation in the tick species.
31936588	9	12	from	apparatus	1457:1465	arg1	stages					1414:1419	the later stages	1404:1419	the later stages of N-glycan maturation in the Golgi apparatus	1404:1465	While the enzymes required for the early steps of the N-glycosylation pathway are quite conserved, the enzymes involved in the later stages of N-glycan maturation in the Golgi apparatus are highly diverged from those of insects.
31936588	4	13	theme	male	664:667	arg1	glands					689:694	male and female salivary glands	664:694	male and female salivary glands at three different feeding stages	664:728	To assess N-glycome of Amblyomma americanum, we examined the N-glycan structures in male and female salivary glands at three different feeding stages and in carcasses of partially fed lone star ticks.
31936588	1	14	theme	numerous	185:192	arg1	pathogens					211:219	numerous human and animal pathogens	185:219	numerous human and animal pathogens	185:219	Ticks are important ectoparasites and vectors of numerous human and animal pathogens.
31936588	2	15	theme	host	298:301	arg1	system					311:316	the host defense system	294:316	the host defense system	294:316	Ticks secrete saliva that contains various bioactive materials to evade the host defense system, and often facilitates the pathogen transmission.
31936588	0	16	from	Glands	84:89	arg1	americanum					124:133	Amblyomma americanum	114:133	Amblyomma americanum	114:133	Alpha-Gal and Cross-Reactive Carbohydrate Determinants in the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum.
31936588	0	16	from	Glands	84:89	arg1	Tick					108:111	the Lone Star Tick	94:111	the Lone Star Tick	94:111	Alpha-Gal and Cross-Reactive Carbohydrate Determinants in the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum.
31936588	7	17	theme	basal	1124:1128	arg1	GlcNac					1130:1135	the basal GlcNac	1120:1135	the basal GlcNac	1120:1135	These CCDs include xylosylation of the core mannose, 1,3-mono and 1,3- and 1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal.
31936588	1	18	theme	pathogens	211:219	arg1	pathogens					211:219	numerous human and animal pathogens	185:219	numerous human and animal pathogens	185:219	Ticks are important ectoparasites and vectors of numerous human and animal pathogens.
31936588	1	18	theme	pathogens	211:219	arg1	vectors					174:180	vectors	174:180	vectors	174:180	Ticks are important ectoparasites and vectors of numerous human and animal pathogens.
31936588	1	18	theme	pathogens	211:219	arg1	Ticks					136:140	Ticks	136:140	Ticks	136:140	Ticks are important ectoparasites and vectors of numerous human and animal pathogens.
31936588	1	18	theme	pathogens	211:219	arg1	ectoparasites					156:168	ectoparasites	156:168	ectoparasites	156:168	Ticks are important ectoparasites and vectors of numerous human and animal pathogens.
31936588	8	19	theme	xylosylation	1189:1200	arg1	aGal					1232:1235	aGal	1232:1235	aGal	1232:1235	Combinations of both xylosylation and aGal and fucosylation and aGal were also found on the N-glycan structures.
31936588	8	19	theme	xylosylation	1189:1200	arg1	fucosylation					1215:1226	fucosylation	1215:1226	fucosylation	1215:1226	Combinations of both xylosylation and aGal and fucosylation and aGal were also found on the N-glycan structures.
31936588	8	19	theme	xylosylation	1189:1200	arg1	Combinations					1168:1179	Combinations	1168:1179	Combinations of both xylosylation and aGal	1168:1209	Combinations of both xylosylation and aGal and fucosylation and aGal were also found on the N-glycan structures.
31936588	10	20	from	sensitizer	1667:1676	arg1	host					1701:1704	atypical host human	1692:1710	atypical host human	1692:1710	Most of all, we propose that the aGal serves as a molecular mimicry of bioactive proteins during tick feedings on mammalian hosts, while it contributes as a sensitizer of allergy in atypical host human.
31936588	4	21	theme	feeding	715:721	arg1	stages					723:728	three different feeding stages	699:728	three different feeding stages	699:728	To assess N-glycome of Amblyomma americanum, we examined the N-glycan structures in male and female salivary glands at three different feeding stages and in carcasses of partially fed lone star ticks.
31936588	2	22	theme	various	257:263	arg1	materials					275:283	various bioactive materials	257:283	various bioactive materials	257:283	Ticks secrete saliva that contains various bioactive materials to evade the host defense system, and often facilitates the pathogen transmission.
31936588	8	23	theme	N-glycan	1260:1267	arg1	structures					1269:1278	the N-glycan structures	1256:1278	the N-glycan structures	1256:1278	Combinations of both xylosylation and aGal and fucosylation and aGal were also found on the N-glycan structures.
31936588	4	24	theme	female	673:678	arg1	glands					689:694	male and female salivary glands	664:694	male and female salivary glands at three different feeding stages	664:728	To assess N-glycome of Amblyomma americanum, we examined the N-glycan structures in male and female salivary glands at three different feeding stages and in carcasses of partially fed lone star ticks.
31936588	7	25	theme	GlcNac	1130:1135	arg1	1,6-difucosylations					1097:1115	1,6-difucosylations	1097:1115	1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal	1097:1165	These CCDs include xylosylation of the core mannose, 1,3-mono and 1,3- and 1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal.
31936588	7	25	theme	GlcNac	1130:1135	arg1	xylosylation					1041:1052	xylosylation	1041:1052	xylosylation of the core mannose, 1,3-mono and 1,3-	1041:1091	These CCDs include xylosylation of the core mannose, 1,3-mono and 1,3- and 1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal.
31936588	0	26	theme	Glands	84:89	arg1	N-Glycans					62:70	the N-Glycans	58:70	the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum	58:133	Alpha-Gal and Cross-Reactive Carbohydrate Determinants in the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum.
31936588	9	27	from	those	1492:1496	arg1	diverged					1478:1485	diverged	1478:1485	diverged	1478:1485	While the enzymes required for the early steps of the N-glycosylation pathway are quite conserved, the enzymes involved in the later stages of N-glycan maturation in the Golgi apparatus are highly diverged from those of insects.
31936588	9	27	from	those	1492:1496	arg1	enzymes					1384:1390	the enzymes	1380:1390	the enzymes involved in the later stages of N-glycan maturation in the Golgi apparatus	1380:1465	While the enzymes required for the early steps of the N-glycosylation pathway are quite conserved, the enzymes involved in the later stages of N-glycan maturation in the Golgi apparatus are highly diverged from those of insects.
31936588	6	28	theme	blood	969:973	arg1	feeding					975:981	blood feeding	969:981	blood feeding in both male and female A. americanum	969:1019	The aGal epitopes and cross-reactive carbohydrate determinants (CCD) increases over time after the onset of blood feeding in both male and female A. americanum.
31936588	10	29	theme	tick	1607:1610	arg1	feedings					1612:1619	tick feedings	1607:1619	tick feedings on mammalian hosts	1607:1638	Most of all, we propose that the aGal serves as a molecular mimicry of bioactive proteins during tick feedings on mammalian hosts, while it contributes as a sensitizer of allergy in atypical host human.
31936588	4	30	theme	lone	764:767	arg1	ticks					774:778	partially fed lone star ticks	750:778	partially fed lone star ticks	750:778	To assess N-glycome of Amblyomma americanum, we examined the N-glycan structures in male and female salivary glands at three different feeding stages and in carcasses of partially fed lone star ticks.
31936588	10	31	theme	bioactive	1581:1589	arg1	proteins					1591:1598	bioactive proteins	1581:1598	bioactive proteins	1581:1598	Most of all, we propose that the aGal serves as a molecular mimicry of bioactive proteins during tick feedings on mammalian hosts, while it contributes as a sensitizer of allergy in atypical host human.
31936588	7	32	theme	diantennary	1150:1160	arg1	aGal					1162:1165	mono- or diantennary aGal	1141:1165	mono- or diantennary aGal	1141:1165	These CCDs include xylosylation of the core mannose, 1,3-mono and 1,3- and 1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal.
31936588	6	33	dep	male	991:994	arg1	americanum					1010:1019	A. americanum	1007:1019	A. americanum	1007:1019	The aGal epitopes and cross-reactive carbohydrate determinants (CCD) increases over time after the onset of blood feeding in both male and female A. americanum.
31936588	6	34	from	feeding	975:981	arg1	female					1000:1005	female	1000:1005	female	1000:1005	The aGal epitopes and cross-reactive carbohydrate determinants (CCD) increases over time after the onset of blood feeding in both male and female A. americanum.
31936588	6	34	from	feeding	975:981	arg1	male					991:994	male	991:994	male	991:994	The aGal epitopes and cross-reactive carbohydrate determinants (CCD) increases over time after the onset of blood feeding in both male and female A. americanum.
31936588	0	35	theme	Alpha-Gal	0:8	arg1	Determinants					42:53	Alpha-Gal and Cross-Reactive Carbohydrate Determinants	0:53	Determinants	42:53	Alpha-Gal and Cross-Reactive Carbohydrate Determinants in the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum.
31936588	3	36	theme	red	442:444	arg1	allergy					451:457	red meat allergy	442:457	red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal)	442:577	In addition, the Lone star tick saliva is thought to be the sensitizer in red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal).
31936588	0	37	theme	Cross-Reactive	14:27	arg1	Determinants					42:53	Alpha-Gal and Cross-Reactive Carbohydrate Determinants	0:53	Determinants	42:53	Alpha-Gal and Cross-Reactive Carbohydrate Determinants in the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum.
31936588	10	38	theme	atypical	1692:1699	arg1	host					1701:1704	atypical host human	1692:1710	atypical host human	1692:1710	Most of all, we propose that the aGal serves as a molecular mimicry of bioactive proteins during tick feedings on mammalian hosts, while it contributes as a sensitizer of allergy in atypical host human.
31936588	7	39	theme	mono-	1141:1145	arg1	aGal					1162:1165	mono- or diantennary aGal	1141:1165	mono- or diantennary aGal	1141:1165	These CCDs include xylosylation of the core mannose, 1,3-mono and 1,3- and 1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal.
31936588	9	40	theme	early	1316:1320	arg1	steps					1322:1326	the early steps	1312:1326	the early steps of the N-glycosylation pathway	1312:1357	While the enzymes required for the early steps of the N-glycosylation pathway are quite conserved, the enzymes involved in the later stages of N-glycan maturation in the Golgi apparatus are highly diverged from those of insects.
31936588	3	41	theme	star	390:393	arg1	saliva					400:405	the Lone star tick saliva	381:405	the Lone star tick saliva	381:405	In addition, the Lone star tick saliva is thought to be the sensitizer in red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal).
31936588	3	41	theme	star	390:393	arg1	sensitizer					428:437	the sensitizer	424:437	the sensitizer in red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal)	424:577	In addition, the Lone star tick saliva is thought to be the sensitizer in red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal).
31936588	7	42	gly	1,6-difucosylations	1097:1115	arg1	aGal					1162:1165	mono- or diantennary aGal	1141:1165	mono- or diantennary aGal	1141:1165	These CCDs include xylosylation of the core mannose, 1,3-mono and 1,3- and 1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal.
31936588	7	42	gly	1,6-difucosylations	1097:1115	arg1	GlcNac					1130:1135	the basal GlcNac	1120:1135	the basal GlcNac	1120:1135	These CCDs include xylosylation of the core mannose, 1,3-mono and 1,3- and 1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal.
31936588	7	42	gly	1,6-difucosylations	1097:1115	arg1	1,3-					1088:1091	1,3-	1088:1091	1,3-	1088:1091	These CCDs include xylosylation of the core mannose, 1,3-mono and 1,3- and 1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal.
31936588	7	42	gly	1,6-difucosylations	1097:1115	arg1	mannose					1066:1072	the core mannose	1057:1072	the core mannose	1057:1072	These CCDs include xylosylation of the core mannose, 1,3-mono and 1,3- and 1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal.
31936588	7	42	gly	1,6-difucosylations	1097:1115	arg1	1,3-mono					1075:1082	1,3-mono	1075:1082	1,3-mono	1075:1082	These CCDs include xylosylation of the core mannose, 1,3-mono and 1,3- and 1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal.
31936588	9	43	from	stages	1414:1419	arg1	apparatus					1457:1465	the Golgi apparatus	1447:1465	the Golgi apparatus	1447:1465	While the enzymes required for the early steps of the N-glycosylation pathway are quite conserved, the enzymes involved in the later stages of N-glycan maturation in the Golgi apparatus are highly diverged from those of insects.
31936588	4	44	theme	ticks	774:778	arg1	carcasses					737:745	carcasses	737:745	carcasses of partially fed lone star ticks	737:778	To assess N-glycome of Amblyomma americanum, we examined the N-glycan structures in male and female salivary glands at three different feeding stages and in carcasses of partially fed lone star ticks.
31936588	10	45	theme	mammalian	1624:1632	arg1	hosts					1634:1638	mammalian hosts	1624:1638	mammalian hosts	1624:1638	Most of all, we propose that the aGal serves as a molecular mimicry of bioactive proteins during tick feedings on mammalian hosts, while it contributes as a sensitizer of allergy in atypical host human.
31936588	9	46	theme	N-glycosylation	1335:1349	arg1	pathway					1351:1357	the N-glycosylation pathway	1331:1357	the N-glycosylation pathway	1331:1357	While the enzymes required for the early steps of the N-glycosylation pathway are quite conserved, the enzymes involved in the later stages of N-glycan maturation in the Golgi apparatus are highly diverged from those of insects.
31936588	10	47	theme	proteins	1591:1598	arg1	mimicry					1570:1576	a molecular mimicry	1558:1576	a molecular mimicry of bioactive proteins during tick feedings on mammalian hosts	1558:1638	Most of all, we propose that the aGal serves as a molecular mimicry of bioactive proteins during tick feedings on mammalian hosts, while it contributes as a sensitizer of allergy in atypical host human.
31936588	10	47	theme	proteins	1591:1598	arg1	aGal					1543:1546	the aGal	1539:1546	the aGal	1539:1546	Most of all, we propose that the aGal serves as a molecular mimicry of bioactive proteins during tick feedings on mammalian hosts, while it contributes as a sensitizer of allergy in atypical host human.
31936588	3	48	theme	allergic	487:494	arg1	reaction					496:503	an allergic reaction	484:503	an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal)	484:577	In addition, the Lone star tick saliva is thought to be the sensitizer in red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal).
31936588	10	49	from	feedings	1612:1619	arg1	hosts					1634:1638	mammalian hosts	1624:1638	mammalian hosts	1624:1638	Most of all, we propose that the aGal serves as a molecular mimicry of bioactive proteins during tick feedings on mammalian hosts, while it contributes as a sensitizer of allergy in atypical host human.
31936588	4	50	theme	Amblyomma	603:611	arg1	americanum					613:622	Amblyomma americanum	603:622	Amblyomma americanum	603:622	To assess N-glycome of Amblyomma americanum, we examined the N-glycan structures in male and female salivary glands at three different feeding stages and in carcasses of partially fed lone star ticks.
31936588	6	51	theme	cross-reactive	883:896	arg1	determinants					911:922	cross-reactive carbohydrate determinants	883:922	cross-reactive carbohydrate determinants	883:922	The aGal epitopes and cross-reactive carbohydrate determinants (CCD) increases over time after the onset of blood feeding in both male and female A. americanum.
31936588	10	52	theme	allergy	1681:1687	arg1	sensitizer					1667:1676	a sensitizer	1665:1676	a sensitizer of allergy in atypical host human	1665:1710	Most of all, we propose that the aGal serves as a molecular mimicry of bioactive proteins during tick feedings on mammalian hosts, while it contributes as a sensitizer of allergy in atypical host human.
31936588	7	53	theme	core	1061:1064	arg1	mannose					1066:1072	the core mannose	1057:1072	the core mannose	1057:1072	These CCDs include xylosylation of the core mannose, 1,3-mono and 1,3- and 1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal.
31936588	4	54	theme	N-glycan	641:648	arg1	structures					650:659	the N-glycan structures	637:659	the N-glycan structures in male and female salivary glands at three different feeding stages and in carcasses of partially fed lone star ticks	637:778	To assess N-glycome of Amblyomma americanum, we examined the N-glycan structures in male and female salivary glands at three different feeding stages and in carcasses of partially fed lone star ticks.
31936588	6	55	from	female	1000:1005	arg1	onset					960:964	the onset	956:964	the onset of blood feeding in both male and female A. americanum	956:1019	The aGal epitopes and cross-reactive carbohydrate determinants (CCD) increases over time after the onset of blood feeding in both male and female A. americanum.
31936588	10	56	theme	human	1706:1710	arg1	host					1701:1704	atypical host human	1692:1710	atypical host human	1692:1710	Most of all, we propose that the aGal serves as a molecular mimicry of bioactive proteins during tick feedings on mammalian hosts, while it contributes as a sensitizer of allergy in atypical host human.
31936588	5	57	theme	tick	847:850	arg1	species					852:858	the tick species	843:858	the tick species	843:858	We also surveyed the genes involved in the N-glycosylation in the tick species.
31936588	0	58	theme	Amblyomma	114:122	arg1	americanum					124:133	Amblyomma americanum	114:133	Amblyomma americanum	114:133	Alpha-Gal and Cross-Reactive Carbohydrate Determinants in the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum.
31936588	0	58	theme	Amblyomma	114:122	arg1	Tick					108:111	the Lone Star Tick	94:111	the Lone Star Tick	94:111	Alpha-Gal and Cross-Reactive Carbohydrate Determinants in the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum.
31936588	2	59	theme	defense	303:309	arg1	system					311:316	the host defense system	294:316	the host defense system	294:316	Ticks secrete saliva that contains various bioactive materials to evade the host defense system, and often facilitates the pathogen transmission.
31936588	1	60	theme	human	194:198	arg1	pathogens					211:219	numerous human and animal pathogens	185:219	numerous human and animal pathogens	185:219	Ticks are important ectoparasites and vectors of numerous human and animal pathogens.
31936588	9	61	theme	later	1408:1412	arg1	stages					1414:1419	the later stages	1404:1419	the later stages of N-glycan maturation in the Golgi apparatus	1404:1465	While the enzymes required for the early steps of the N-glycosylation pathway are quite conserved, the enzymes involved in the later stages of N-glycan maturation in the Golgi apparatus are highly diverged from those of insects.
31936588	9	62	theme	N-glycan	1424:1431	arg1	maturation					1433:1442	N-glycan maturation	1424:1442	N-glycan maturation in the Golgi apparatus	1424:1465	While the enzymes required for the early steps of the N-glycosylation pathway are quite conserved, the enzymes involved in the later stages of N-glycan maturation in the Golgi apparatus are highly diverged from those of insects.
31936588	7	63	theme	aGal	1162:1165	arg1	1,6-difucosylations					1097:1115	1,6-difucosylations	1097:1115	1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal	1097:1165	These CCDs include xylosylation of the core mannose, 1,3-mono and 1,3- and 1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal.
31936588	7	63	theme	aGal	1162:1165	arg1	xylosylation					1041:1052	xylosylation	1041:1052	xylosylation of the core mannose, 1,3-mono and 1,3-	1041:1091	These CCDs include xylosylation of the core mannose, 1,3-mono and 1,3- and 1,6-difucosylations of the basal GlcNac and mono- or diantennary aGal.
31936588	8	64	gly	fucosylation	1215:1226	arg1	aGal					1206:1209	aGal	1206:1209	aGal	1206:1209	Combinations of both xylosylation and aGal and fucosylation and aGal were also found on the N-glycan structures.
31936588	8	64	gly	fucosylation	1215:1226	arg1	structures					1269:1278	the N-glycan structures	1256:1278	the N-glycan structures	1256:1278	Combinations of both xylosylation and aGal and fucosylation and aGal were also found on the N-glycan structures.
31936588	8	64	gly	fucosylation	1215:1226	arg1	xylosylation					1189:1200	xylosylation	1189:1200	xylosylation	1189:1200	Combinations of both xylosylation and aGal and fucosylation and aGal were also found on the N-glycan structures.
31936588	5	65	from	N-glycosylation	824:838	arg1	species					852:858	the tick species	843:858	the tick species	843:858	We also surveyed the genes involved in the N-glycosylation in the tick species.
31936588	0	66	from	Tick	108:111	arg1	N-Glycans					62:70	the N-Glycans	58:70	the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum	58:133	Alpha-Gal and Cross-Reactive Carbohydrate Determinants in the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum.
31936588	2	67	theme	bioactive	265:273	arg1	materials					275:283	various bioactive materials	257:283	various bioactive materials	257:283	Ticks secrete saliva that contains various bioactive materials to evade the host defense system, and often facilitates the pathogen transmission.
31936588	9	68	theme	Golgi	1451:1455	arg1	apparatus					1457:1465	the Golgi apparatus	1447:1465	the Golgi apparatus	1447:1465	While the enzymes required for the early steps of the N-glycosylation pathway are quite conserved, the enzymes involved in the later stages of N-glycan maturation in the Golgi apparatus are highly diverged from those of insects.
31936588	3	69	theme	terminal	533:540	arg1	galactose-alpha-1,3-galactose					542:570	terminal galactose-alpha-1,3-galactose	533:570	terminal galactose-alpha-1,3-galactose (aGal)	533:577	In addition, the Lone star tick saliva is thought to be the sensitizer in red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal).
31936588	3	69	theme	terminal	533:540	arg1	aGal					573:576	aGal	573:576	aGal	573:576	In addition, the Lone star tick saliva is thought to be the sensitizer in red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal).
31936588	4	70	theme	different	705:713	arg1	stages					723:728	three different feeding stages	699:728	three different feeding stages	699:728	To assess N-glycome of Amblyomma americanum, we examined the N-glycan structures in male and female salivary glands at three different feeding stages and in carcasses of partially fed lone star ticks.
31936588	0	71	theme	Salivary	75:82	arg1	Glands					84:89	Salivary Glands	75:89	Salivary Glands in the Lone Star Tick, Amblyomma americanum	75:133	Alpha-Gal and Cross-Reactive Carbohydrate Determinants in the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum.
31936588	1	72	theme	important	146:154	arg1	pathogens					211:219	numerous human and animal pathogens	185:219	numerous human and animal pathogens	185:219	Ticks are important ectoparasites and vectors of numerous human and animal pathogens.
31936588	1	72	theme	important	146:154	arg1	Ticks					136:140	Ticks	136:140	Ticks	136:140	Ticks are important ectoparasites and vectors of numerous human and animal pathogens.
31936588	1	72	theme	important	146:154	arg1	ectoparasites					156:168	ectoparasites	156:168	ectoparasites	156:168	Ticks are important ectoparasites and vectors of numerous human and animal pathogens.
31936588	6	73	theme	feeding	975:981	arg1	onset					960:964	the onset	956:964	the onset of blood feeding in both male and female A. americanum	956:1019	The aGal epitopes and cross-reactive carbohydrate determinants (CCD) increases over time after the onset of blood feeding in both male and female A. americanum.
31936588	4	74	theme	salivary	680:687	arg1	glands					689:694	male and female salivary glands	664:694	male and female salivary glands at three different feeding stages	664:728	To assess N-glycome of Amblyomma americanum, we examined the N-glycan structures in male and female salivary glands at three different feeding stages and in carcasses of partially fed lone star ticks.
31936588	0	75	theme	Lone	98:101	arg1	americanum					124:133	Amblyomma americanum	114:133	Amblyomma americanum	114:133	Alpha-Gal and Cross-Reactive Carbohydrate Determinants in the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum.
31936588	0	75	theme	Lone	98:101	arg1	Tick					108:111	the Lone Star Tick	94:111	the Lone Star Tick	94:111	Alpha-Gal and Cross-Reactive Carbohydrate Determinants in the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum.
31936588	0	76	from	N-Glycans	62:70	arg1	americanum					124:133	Amblyomma americanum	114:133	Amblyomma americanum	114:133	Alpha-Gal and Cross-Reactive Carbohydrate Determinants in the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum.
31936588	0	76	from	N-Glycans	62:70	arg1	Tick					108:111	the Lone Star Tick	94:111	the Lone Star Tick	94:111	Alpha-Gal and Cross-Reactive Carbohydrate Determinants in the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum.
31936588	6	77	from	male	991:994	arg1	onset					960:964	the onset	956:964	the onset of blood feeding in both male and female A. americanum	956:1019	The aGal epitopes and cross-reactive carbohydrate determinants (CCD) increases over time after the onset of blood feeding in both male and female A. americanum.
31936588	6	78	from	onset	960:964	arg1	female					1000:1005	female	1000:1005	female	1000:1005	The aGal epitopes and cross-reactive carbohydrate determinants (CCD) increases over time after the onset of blood feeding in both male and female A. americanum.
31936588	6	78	from	onset	960:964	arg1	male					991:994	male	991:994	male	991:994	The aGal epitopes and cross-reactive carbohydrate determinants (CCD) increases over time after the onset of blood feeding in both male and female A. americanum.
31936588	4	79	theme	fed	760:762	arg1	ticks					774:778	partially fed lone star ticks	750:778	partially fed lone star ticks	750:778	To assess N-glycome of Amblyomma americanum, we examined the N-glycan structures in male and female salivary glands at three different feeding stages and in carcasses of partially fed lone star ticks.
31936588	3	80	theme	Lone	385:388	arg1	saliva					400:405	the Lone star tick saliva	381:405	the Lone star tick saliva	381:405	In addition, the Lone star tick saliva is thought to be the sensitizer in red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal).
31936588	3	80	theme	Lone	385:388	arg1	sensitizer					428:437	the sensitizer	424:437	the sensitizer in red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal)	424:577	In addition, the Lone star tick saliva is thought to be the sensitizer in red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal).
31936588	10	81	theme	molecular	1560:1568	arg1	mimicry					1570:1576	a molecular mimicry	1558:1576	a molecular mimicry of bioactive proteins during tick feedings on mammalian hosts	1558:1638	Most of all, we propose that the aGal serves as a molecular mimicry of bioactive proteins during tick feedings on mammalian hosts, while it contributes as a sensitizer of allergy in atypical host human.
31936588	10	81	theme	molecular	1560:1568	arg1	aGal					1543:1546	the aGal	1539:1546	the aGal	1539:1546	Most of all, we propose that the aGal serves as a molecular mimicry of bioactive proteins during tick feedings on mammalian hosts, while it contributes as a sensitizer of allergy in atypical host human.
31936588	0	82	theme	Carbohydrate	29:40	arg1	Determinants					42:53	Alpha-Gal and Cross-Reactive Carbohydrate Determinants	0:53	Determinants	42:53	Alpha-Gal and Cross-Reactive Carbohydrate Determinants in the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum.
31936588	8	83	located	found	1247:1251	arg2	fucosylation					1215:1226	fucosylation	1215:1226	fucosylation	1215:1226	Combinations of both xylosylation and aGal and fucosylation and aGal were also found on the N-glycan structures.
31936588	8	83	located	found	1247:1251	arg2	Combinations					1168:1179	Combinations	1168:1179	Combinations of both xylosylation and aGal	1168:1209	Combinations of both xylosylation and aGal and fucosylation and aGal were also found on the N-glycan structures.
31936588	8	83	located	found	1247:1251	arg1	structures					1269:1278	the N-glycan structures	1256:1278	the N-glycan structures	1256:1278	Combinations of both xylosylation and aGal and fucosylation and aGal were also found on the N-glycan structures.
31936588	4	84	from	stages	723:728	arg1	structures					650:659	the N-glycan structures	637:659	the N-glycan structures in male and female salivary glands at three different feeding stages and in carcasses of partially fed lone star ticks	637:778	To assess N-glycome of Amblyomma americanum, we examined the N-glycan structures in male and female salivary glands at three different feeding stages and in carcasses of partially fed lone star ticks.
31936588	4	84	from	stages	723:728	arg1	glands					689:694	male and female salivary glands	664:694	male and female salivary glands at three different feeding stages	664:728	To assess N-glycome of Amblyomma americanum, we examined the N-glycan structures in male and female salivary glands at three different feeding stages and in carcasses of partially fed lone star ticks.
31936588	3	85	theme	meat	446:449	arg1	allergy					451:457	red meat allergy	442:457	red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal)	442:577	In addition, the Lone star tick saliva is thought to be the sensitizer in red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal).
31936588	1	86	theme	animal	204:209	arg1	pathogens					211:219	numerous human and animal pathogens	185:219	numerous human and animal pathogens	185:219	Ticks are important ectoparasites and vectors of numerous human and animal pathogens.
31936588	4	87	theme	americanum	613:622	arg1	N-glycome					590:598	N-glycome	590:598	N-glycome of Amblyomma americanum	590:622	To assess N-glycome of Amblyomma americanum, we examined the N-glycan structures in male and female salivary glands at three different feeding stages and in carcasses of partially fed lone star ticks.
31936588	3	88	from	sensitizer	428:437	arg1	allergy					451:457	red meat allergy	442:457	red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal)	442:577	In addition, the Lone star tick saliva is thought to be the sensitizer in red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal).
31936588	0	89	theme	Star	103:106	arg1	americanum					124:133	Amblyomma americanum	114:133	Amblyomma americanum	114:133	Alpha-Gal and Cross-Reactive Carbohydrate Determinants in the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum.
31936588	0	89	theme	Star	103:106	arg1	Tick					108:111	the Lone Star Tick	94:111	the Lone Star Tick	94:111	Alpha-Gal and Cross-Reactive Carbohydrate Determinants in the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum.
31936588	9	90	from	maturation	1433:1442	arg1	apparatus					1457:1465	the Golgi apparatus	1447:1465	the Golgi apparatus	1447:1465	While the enzymes required for the early steps of the N-glycosylation pathway are quite conserved, the enzymes involved in the later stages of N-glycan maturation in the Golgi apparatus are highly diverged from those of insects.
31936588	3	91	theme	tick	395:398	arg1	saliva					400:405	the Lone star tick saliva	381:405	the Lone star tick saliva	381:405	In addition, the Lone star tick saliva is thought to be the sensitizer in red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal).
31936588	3	91	theme	tick	395:398	arg1	sensitizer					428:437	the sensitizer	424:437	the sensitizer in red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal)	424:577	In addition, the Lone star tick saliva is thought to be the sensitizer in red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal).
31936588	3	92	contain	carrying	524:531	arg1	moieties					515:522	glycan moieties	508:522	glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal)	508:577	In addition, the Lone star tick saliva is thought to be the sensitizer in red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal).
31936588	3	92	contain	carrying	524:531	arg2	aGal					573:576	aGal	573:576	aGal	573:576	In addition, the Lone star tick saliva is thought to be the sensitizer in red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal).
31936588	3	92	contain	carrying	524:531	arg2	galactose-alpha-1,3-galactose					542:570	terminal galactose-alpha-1,3-galactose	533:570	terminal galactose-alpha-1,3-galactose (aGal)	533:577	In addition, the Lone star tick saliva is thought to be the sensitizer in red meat allergy that is characterized by an allergic reaction to glycan moieties carrying terminal galactose-alpha-1,3-galactose (aGal).
31936588	4	93	theme	star	769:772	arg1	ticks					774:778	partially fed lone star ticks	750:778	partially fed lone star ticks	750:778	To assess N-glycome of Amblyomma americanum, we examined the N-glycan structures in male and female salivary glands at three different feeding stages and in carcasses of partially fed lone star ticks.
31936588	9	94	theme	pathway	1351:1357	arg1	steps					1322:1326	the early steps	1312:1326	the early steps of the N-glycosylation pathway	1312:1357	While the enzymes required for the early steps of the N-glycosylation pathway are quite conserved, the enzymes involved in the later stages of N-glycan maturation in the Golgi apparatus are highly diverged from those of insects.
31936588	9	95	theme	maturation	1433:1442	arg1	stages					1414:1419	the later stages	1404:1419	the later stages of N-glycan maturation in the Golgi apparatus	1404:1465	While the enzymes required for the early steps of the N-glycosylation pathway are quite conserved, the enzymes involved in the later stages of N-glycan maturation in the Golgi apparatus are highly diverged from those of insects.
31936588	6	96	dep	epitopes	870:877	arg1	The					861:863	The	861:863	The	861:863	The aGal epitopes and cross-reactive carbohydrate determinants (CCD) increases over time after the onset of blood feeding in both male and female A. americanum.
32553965	0	0	theme	degradation	93:103	arg1	product					105:111	the degradation product	89:111	the degradation product	89:111	Enzymatic hydrolysis of polysaccharide from Auricularia auricula and characterization of the degradation product.
32553965	5	1	theme	reducing	735:742	arg1	sugar					744:748	reducing sugar	735:748	reducing sugar	735:748	Under these conditions, the actual yield of reducing sugar was 16.50 mg/mL and the DPPH radical scavenging rate was 87.97%.
32553965	4	2	theme	enzymolysis	579:589	arg1	conditions					591:600	The resulting optimal enzymolysis conditions	557:600	The resulting optimal enzymolysis conditions	557:600	The resulting optimal enzymolysis conditions were as follows: enzyme dosage, 13,500 U/g; enzymolysis temperature, 50 °C; and pH, 4.2.
32553965	5	3	theme	sugar	744:748	arg1	16.50 mg/mL					754:764	16.50 mg/mL	754:764	16.50 mg/mL	754:764	Under these conditions, the actual yield of reducing sugar was 16.50 mg/mL and the DPPH radical scavenging rate was 87.97%.
32553965	5	3	theme	sugar	744:748	arg1	yield					726:730	the actual yield	715:730	the actual yield of reducing sugar	715:748	Under these conditions, the actual yield of reducing sugar was 16.50 mg/mL and the DPPH radical scavenging rate was 87.97%.
32553965	9	4	theme	polysaccharide	1262:1275	arg1	activity					1246:1253	The antioxidant activity	1230:1253	The antioxidant activity of the polysaccharide	1230:1275	The antioxidant activity of the polysaccharide indicated that C-EAAP had better antioxidant activity than AAP.
32553965	9	5	theme	better	1303:1308	arg1	activity					1322:1329	better antioxidant activity	1303:1329	better antioxidant activity	1303:1329	The antioxidant activity of the polysaccharide indicated that C-EAAP had better antioxidant activity than AAP.
32553965	4	6	theme	optimal	571:577	arg1	conditions					591:600	The resulting optimal enzymolysis conditions	557:600	The resulting optimal enzymolysis conditions	557:600	The resulting optimal enzymolysis conditions were as follows: enzyme dosage, 13,500 U/g; enzymolysis temperature, 50 °C; and pH, 4.2.
32553965	1	7	theme	AAP	279:281	arg1	product					268:274	the degradation product	252:274	the degradation product of AAP	252:281	An efficient enzymatic hydrolysis method was developed and optimized for the degradation of auricularia auricula polysaccharide (AAP) and the degradation product of AAP was characterized.
32553965	0	8	theme	product	105:111	arg1	characterization					69:84	characterization	69:84	characterization	69:84	Enzymatic hydrolysis of polysaccharide from Auricularia auricula and characterization of the degradation product.
32553965	0	8	theme	product	105:111	arg1	auricula					56:63	Auricularia auricula	44:63	Auricularia auricula	44:63	Enzymatic hydrolysis of polysaccharide from Auricularia auricula and characterization of the degradation product.
32553965	9	9	theme	antioxidant	1234:1244	arg1	activity					1246:1253	The antioxidant activity	1230:1253	The antioxidant activity of the polysaccharide	1230:1275	The antioxidant activity of the polysaccharide indicated that C-EAAP had better antioxidant activity than AAP.
32553965	10	10	theme	anion	1415:1419	arg1	O2-·					1431:1434	O2-·	1431:1434	O2-·	1431:1434	The scavenging rates of C-EAAP for hydroxyl radicals (·OH) and superoxide anion radicals (O2-·) were 1.65 and 1.90 times those of AAP.
32553965	10	10	theme	anion	1415:1419	arg1	radicals					1421:1428	superoxide anion radicals	1404:1428	superoxide anion radicals (O2-·)	1404:1435	The scavenging rates of C-EAAP for hydroxyl radicals (·OH) and superoxide anion radicals (O2-·) were 1.65 and 1.90 times those of AAP.
32553965	0	11	from	auricula	56:63	arg1	hydrolysis					10:19	Enzymatic hydrolysis	0:19	Enzymatic hydrolysis of polysaccharide from Auricularia auricula and characterization of the degradation product.	0:112	Enzymatic hydrolysis of polysaccharide from Auricularia auricula and characterization of the degradation product.
32553965	9	12	contain	had	1299:1301	arg1	C-EAAP					1292:1297	C-EAAP	1292:1297	C-EAAP	1292:1297	The antioxidant activity of the polysaccharide indicated that C-EAAP had better antioxidant activity than AAP.
32553965	9	12	contain	had	1299:1301	arg2	activity					1322:1329	better antioxidant activity	1303:1329	better antioxidant activity	1303:1329	The antioxidant activity of the polysaccharide indicated that C-EAAP had better antioxidant activity than AAP.
32553965	3	13	theme	surface	530:536	arg1	RSM					551:553	RSM	551:553	RSM	551:553	The yield of reducing sugar and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate were used as indices to optimize the enzymatic hydrolysis of AAP, based on response surface methodology (RSM).
32553965	3	13	theme	surface	530:536	arg1	methodology					538:548	response surface methodology	521:548	response surface methodology (RSM)	521:554	The yield of reducing sugar and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate were used as indices to optimize the enzymatic hydrolysis of AAP, based on response surface methodology (RSM).
32553965	5	14	theme	actual	719:724	arg1	16.50 mg/mL					754:764	16.50 mg/mL	754:764	16.50 mg/mL	754:764	Under these conditions, the actual yield of reducing sugar was 16.50 mg/mL and the DPPH radical scavenging rate was 87.97%.
32553965	5	14	theme	actual	719:724	arg1	yield					726:730	the actual yield	715:730	the actual yield of reducing sugar	715:748	Under these conditions, the actual yield of reducing sugar was 16.50 mg/mL and the DPPH radical scavenging rate was 87.97%.
32553965	3	15	theme	radical	422:428	arg1	rate					441:444	radical scavenging rate	422:444	radical scavenging rate	422:444	The yield of reducing sugar and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate were used as indices to optimize the enzymatic hydrolysis of AAP, based on response surface methodology (RSM).
32553965	3	16	used	used	451:454	arg2	yield					353:357	The yield	349:357	The yield of reducing sugar and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate	349:444	The yield of reducing sugar and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate were used as indices to optimize the enzymatic hydrolysis of AAP, based on response surface methodology (RSM).
32553965	3	16	used	used	451:454	arg2	indices					459:465	indices	459:465	indices	459:465	The yield of reducing sugar and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate were used as indices to optimize the enzymatic hydrolysis of AAP, based on response surface methodology (RSM).
32553965	4	17	theme	enzymolysis	646:656	arg1	temperature					658:668	enzymolysis temperature	646:668	enzymolysis temperature	646:668	The resulting optimal enzymolysis conditions were as follows: enzyme dosage, 13,500 U/g; enzymolysis temperature, 50 °C; and pH, 4.2.
32553965	4	17	theme	enzymolysis	646:656	arg1	dosage					626:631	enzyme dosage	619:631	enzyme dosage	619:631	The resulting optimal enzymolysis conditions were as follows: enzyme dosage, 13,500 U/g; enzymolysis temperature, 50 °C; and pH, 4.2.
32553965	5	18	theme	DPPH	774:777	arg1	%					812:812	87.97%	807:812	87.97%	807:812	Under these conditions, the actual yield of reducing sugar was 16.50 mg/mL and the DPPH radical scavenging rate was 87.97%.
32553965	5	18	theme	DPPH	774:777	arg1	rate					798:801	the DPPH radical scavenging rate	770:801	the DPPH radical scavenging rate	770:801	Under these conditions, the actual yield of reducing sugar was 16.50 mg/mL and the DPPH radical scavenging rate was 87.97%.
32553965	6	19	theme	AAP	842:844	arg1	homogeneous					859:869	homogeneous	859:869	homogeneous	859:869	The degradation product of AAP (C-EAAP) was homogeneous and contained alpha and beta glycoside bonds, but did not contain protein or nucleic acid.
32553965	6	19	theme	AAP	842:844	arg1	product					831:837	The degradation product	815:837	The degradation product of AAP (C-EAAP)	815:853	The degradation product of AAP (C-EAAP) was homogeneous and contained alpha and beta glycoside bonds, but did not contain protein or nucleic acid.
32553965	10	20	theme	superoxide	1404:1413	arg1	O2-·					1431:1434	O2-·	1431:1434	O2-·	1431:1434	The scavenging rates of C-EAAP for hydroxyl radicals (·OH) and superoxide anion radicals (O2-·) were 1.65 and 1.90 times those of AAP.
32553965	10	20	theme	superoxide	1404:1413	arg1	radicals					1421:1428	superoxide anion radicals	1404:1428	superoxide anion radicals (O2-·)	1404:1435	The scavenging rates of C-EAAP for hydroxyl radicals (·OH) and superoxide anion radicals (O2-·) were 1.65 and 1.90 times those of AAP.
32553965	0	21	theme	Enzymatic	0:8	arg1	hydrolysis					10:19	Enzymatic hydrolysis	0:19	Enzymatic hydrolysis of polysaccharide from Auricularia auricula and characterization of the degradation product.	0:112	Enzymatic hydrolysis of polysaccharide from Auricularia auricula and characterization of the degradation product.
32553965	6	22	contain	contain	929:935	arg1	homogeneous					859:869	homogeneous	859:869	homogeneous	859:869	The degradation product of AAP (C-EAAP) was homogeneous and contained alpha and beta glycoside bonds, but did not contain protein or nucleic acid.
32553965	6	22	contain	contain	929:935	arg2	acid					956:959	nucleic acid	948:959	nucleic acid	948:959	The degradation product of AAP (C-EAAP) was homogeneous and contained alpha and beta glycoside bonds, but did not contain protein or nucleic acid.
32553965	6	22	contain	contain	929:935	arg1	product					831:837	The degradation product	815:837	The degradation product of AAP (C-EAAP)	815:853	The degradation product of AAP (C-EAAP) was homogeneous and contained alpha and beta glycoside bonds, but did not contain protein or nucleic acid.
32553965	6	22	contain	contain	929:935	arg2	protein					937:943	protein	937:943	protein	937:943	The degradation product of AAP (C-EAAP) was homogeneous and contained alpha and beta glycoside bonds, but did not contain protein or nucleic acid.
32553965	1	23	theme	auricularia	206:216	arg1	polysaccharide					227:240	auricularia auricula polysaccharide	206:240	auricularia auricula polysaccharide (AAP)	206:246	An efficient enzymatic hydrolysis method was developed and optimized for the degradation of auricularia auricula polysaccharide (AAP) and the degradation product of AAP was characterized.
32553965	1	23	theme	auricularia	206:216	arg1	AAP					243:245	AAP	243:245	AAP	243:245	An efficient enzymatic hydrolysis method was developed and optimized for the degradation of auricularia auricula polysaccharide (AAP) and the degradation product of AAP was characterized.
32553965	0	24	theme	polysaccharide	24:37	arg1	hydrolysis					10:19	Enzymatic hydrolysis	0:19	Enzymatic hydrolysis of polysaccharide from Auricularia auricula and characterization of the degradation product.	0:112	Enzymatic hydrolysis of polysaccharide from Auricularia auricula and characterization of the degradation product.
32553965	5	25	theme	radical	779:785	arg1	%					812:812	87.97%	807:812	87.97%	807:812	Under these conditions, the actual yield of reducing sugar was 16.50 mg/mL and the DPPH radical scavenging rate was 87.97%.
32553965	5	25	theme	radical	779:785	arg1	rate					798:801	the DPPH radical scavenging rate	770:801	the DPPH radical scavenging rate	770:801	Under these conditions, the actual yield of reducing sugar was 16.50 mg/mL and the DPPH radical scavenging rate was 87.97%.
32553965	8	26	theme	Monosaccharide	1029:1042	arg1	analysis					1056:1063	Monosaccharide composition analysis	1029:1063	Monosaccharide composition analysis	1029:1063	Monosaccharide composition analysis revealed that C-EAAP was composed of mannose (57.1%), glucuronic acid (10.0%), rhamnose (0.4%), glucose (22.5%), galactose (2.9%), xylose (6.0%), and fucose (1.1%).
32553965	1	27	theme	auricula	218:225	arg1	polysaccharide					227:240	auricularia auricula polysaccharide	206:240	auricularia auricula polysaccharide (AAP)	206:246	An efficient enzymatic hydrolysis method was developed and optimized for the degradation of auricularia auricula polysaccharide (AAP) and the degradation product of AAP was characterized.
32553965	1	27	theme	auricula	218:225	arg1	AAP					243:245	AAP	243:245	AAP	243:245	An efficient enzymatic hydrolysis method was developed and optimized for the degradation of auricularia auricula polysaccharide (AAP) and the degradation product of AAP was characterized.
32553965	4	28	theme	resulting	561:569	arg1	conditions					591:600	The resulting optimal enzymolysis conditions	557:600	The resulting optimal enzymolysis conditions	557:600	The resulting optimal enzymolysis conditions were as follows: enzyme dosage, 13,500 U/g; enzymolysis temperature, 50 °C; and pH, 4.2.
32553965	1	29	theme	efficient	117:125	arg1	method					148:153	An efficient enzymatic hydrolysis method	114:153	An efficient enzymatic hydrolysis method	114:153	An efficient enzymatic hydrolysis method was developed and optimized for the degradation of auricularia auricula polysaccharide (AAP) and the degradation product of AAP was characterized.
32553965	1	30	theme	polysaccharide	227:240	arg1	degradation					191:201	the degradation	187:201	the degradation of auricularia auricula polysaccharide (AAP)	187:246	An efficient enzymatic hydrolysis method was developed and optimized for the degradation of auricularia auricula polysaccharide (AAP) and the degradation product of AAP was characterized.
32553965	0	31	theme	Auricularia	44:54	arg1	auricula					56:63	Auricularia auricula	44:63	Auricularia auricula	44:63	Enzymatic hydrolysis of polysaccharide from Auricularia auricula and characterization of the degradation product.
32553965	3	32	theme	AAP	507:509	arg1	hydrolysis					493:502	the enzymatic hydrolysis	479:502	the enzymatic hydrolysis	479:502	The yield of reducing sugar and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate were used as indices to optimize the enzymatic hydrolysis of AAP, based on response surface methodology (RSM).
32553965	7	33	theme	degradation	990:1000	arg1	product					1002:1008	the degradation product	986:1008	the degradation product	986:1008	The molecular weight of the degradation product was 5.94 × 105 Da.
32553965	6	34	contain	contained	875:883	arg1	homogeneous					859:869	homogeneous	859:869	homogeneous	859:869	The degradation product of AAP (C-EAAP) was homogeneous and contained alpha and beta glycoside bonds, but did not contain protein or nucleic acid.
32553965	6	34	contain	contained	875:883	arg1	product					831:837	The degradation product	815:837	The degradation product of AAP (C-EAAP)	815:853	The degradation product of AAP (C-EAAP) was homogeneous and contained alpha and beta glycoside bonds, but did not contain protein or nucleic acid.
32553965	6	34	contain	contained	875:883	arg2	bonds					910:914	alpha and beta glycoside bonds	885:914	alpha and beta glycoside bonds	885:914	The degradation product of AAP (C-EAAP) was homogeneous and contained alpha and beta glycoside bonds, but did not contain protein or nucleic acid.
32553965	3	35	theme	scavenging	430:439	arg1	rate					441:444	radical scavenging rate	422:444	radical scavenging rate	422:444	The yield of reducing sugar and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate were used as indices to optimize the enzymatic hydrolysis of AAP, based on response surface methodology (RSM).
32553965	6	36	theme	glycoside	900:908	arg1	bonds					910:914	alpha and beta glycoside bonds	885:914	alpha and beta glycoside bonds	885:914	The degradation product of AAP (C-EAAP) was homogeneous and contained alpha and beta glycoside bonds, but did not contain protein or nucleic acid.
32553965	7	37	theme	product	1002:1008	arg1	weight					976:981	The molecular weight	962:981	The molecular weight of the degradation product	962:1008	The molecular weight of the degradation product was 5.94 × 105 Da.
32553965	7	37	theme	product	1002:1008	arg1	5.94 × 105 Da					1014:1026	5.94 × 105 Da	1014:1026	5.94 × 105 Da	1014:1026	The molecular weight of the degradation product was 5.94 × 105 Da.
32553965	6	38	theme	degradation	819:829	arg1	homogeneous					859:869	homogeneous	859:869	homogeneous	859:869	The degradation product of AAP (C-EAAP) was homogeneous and contained alpha and beta glycoside bonds, but did not contain protein or nucleic acid.
32553965	6	38	theme	degradation	819:829	arg1	product					831:837	The degradation product	815:837	The degradation product of AAP (C-EAAP)	815:853	The degradation product of AAP (C-EAAP) was homogeneous and contained alpha and beta glycoside bonds, but did not contain protein or nucleic acid.
32553965	5	39	theme	scavenging	787:796	arg1	%					812:812	87.97%	807:812	87.97%	807:812	Under these conditions, the actual yield of reducing sugar was 16.50 mg/mL and the DPPH radical scavenging rate was 87.97%.
32553965	5	39	theme	scavenging	787:796	arg1	rate					798:801	the DPPH radical scavenging rate	770:801	the DPPH radical scavenging rate	770:801	Under these conditions, the actual yield of reducing sugar was 16.50 mg/mL and the DPPH radical scavenging rate was 87.97%.
32553965	10	40	theme	hydroxyl	1376:1383	arg1	radicals					1385:1392	hydroxyl radicals	1376:1392	hydroxyl radicals (·OH)	1376:1398	The scavenging rates of C-EAAP for hydroxyl radicals (·OH) and superoxide anion radicals (O2-·) were 1.65 and 1.90 times those of AAP.
32553965	10	40	theme	hydroxyl	1376:1383	arg1	·OH					1395:1397	·OH	1395:1397	·OH	1395:1397	The scavenging rates of C-EAAP for hydroxyl radicals (·OH) and superoxide anion radicals (O2-·) were 1.65 and 1.90 times those of AAP.
32553965	6	41	theme	beta	895:898	arg1	bonds					910:914	alpha and beta glycoside bonds	885:914	alpha and beta glycoside bonds	885:914	The degradation product of AAP (C-EAAP) was homogeneous and contained alpha and beta glycoside bonds, but did not contain protein or nucleic acid.
32553965	10	42	theme	scavenging	1345:1354	arg1	rates					1356:1360	The scavenging rates	1341:1360	The scavenging rates of C-EAAP for hydroxyl radicals (·OH) and superoxide anion radicals (O2-·)	1341:1435	The scavenging rates of C-EAAP for hydroxyl radicals (·OH) and superoxide anion radicals (O2-·) were 1.65 and 1.90 times those of AAP.
32553965	10	42	theme	scavenging	1345:1354	arg1	times					1456:1460	1.65 and 1.90 times	1442:1460	1.65 and 1.90 times those of AAP	1442:1473	The scavenging rates of C-EAAP for hydroxyl radicals (·OH) and superoxide anion radicals (O2-·) were 1.65 and 1.90 times those of AAP.
32553965	0	43	from	characterization	69:84	arg1	hydrolysis					10:19	Enzymatic hydrolysis	0:19	Enzymatic hydrolysis of polysaccharide from Auricularia auricula and characterization of the degradation product.	0:112	Enzymatic hydrolysis of polysaccharide from Auricularia auricula and characterization of the degradation product.
32553965	1	44	theme	enzymatic	127:135	arg1	method					148:153	An efficient enzymatic hydrolysis method	114:153	An efficient enzymatic hydrolysis method	114:153	An efficient enzymatic hydrolysis method was developed and optimized for the degradation of auricularia auricula polysaccharide (AAP) and the degradation product of AAP was characterized.
32553965	8	45	theme	composition	1044:1054	arg1	analysis					1056:1063	Monosaccharide composition analysis	1029:1063	Monosaccharide composition analysis	1029:1063	Monosaccharide composition analysis revealed that C-EAAP was composed of mannose (57.1%), glucuronic acid (10.0%), rhamnose (0.4%), glucose (22.5%), galactose (2.9%), xylose (6.0%), and fucose (1.1%).
32553965	1	46	theme	hydrolysis	137:146	arg1	method					148:153	An efficient enzymatic hydrolysis method	114:153	An efficient enzymatic hydrolysis method	114:153	An efficient enzymatic hydrolysis method was developed and optimized for the degradation of auricularia auricula polysaccharide (AAP) and the degradation product of AAP was characterized.
32553965	2	47	used	used	316:319	arg2	Cellulase					302:310	Cellulase	302:310	Cellulase	302:310	Cellulase was used for the degradation of AAP.
32553965	6	48	theme	alpha	885:889	arg1	bonds					910:914	alpha and beta glycoside bonds	885:914	alpha and beta glycoside bonds	885:914	The degradation product of AAP (C-EAAP) was homogeneous and contained alpha and beta glycoside bonds, but did not contain protein or nucleic acid.
32553965	7	49	theme	molecular	966:974	arg1	weight					976:981	The molecular weight	962:981	The molecular weight of the degradation product	962:1008	The molecular weight of the degradation product was 5.94 × 105 Da.
32553965	7	49	theme	molecular	966:974	arg1	5.94 × 105 Da					1014:1026	5.94 × 105 Da	1014:1026	5.94 × 105 Da	1014:1026	The molecular weight of the degradation product was 5.94 × 105 Da.
32553965	10	50	dep	times	1456:1460	arg1	those					1462:1466	those	1462:1466	those	1462:1466	The scavenging rates of C-EAAP for hydroxyl radicals (·OH) and superoxide anion radicals (O2-·) were 1.65 and 1.90 times those of AAP.
32553965	2	51	theme	AAP	344:346	arg1	degradation					329:339	the degradation	325:339	the degradation of AAP	325:346	Cellulase was used for the degradation of AAP.
32553965	3	52	dep	sugar	371:375	arg1	rate					441:444	radical scavenging rate	422:444	radical scavenging rate	422:444	The yield of reducing sugar and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate were used as indices to optimize the enzymatic hydrolysis of AAP, based on response surface methodology (RSM).
32553965	8	53	theme	glucuronic	1119:1128	arg1	acid					1130:1133	glucuronic acid	1119:1133	glucuronic acid (10.0%)	1119:1141	Monosaccharide composition analysis revealed that C-EAAP was composed of mannose (57.1%), glucuronic acid (10.0%), rhamnose (0.4%), glucose (22.5%), galactose (2.9%), xylose (6.0%), and fucose (1.1%).
32553965	8	53	theme	glucuronic	1119:1128	arg1	%					1140:1140	10.0%	1136:1140	10.0%	1136:1140	Monosaccharide composition analysis revealed that C-EAAP was composed of mannose (57.1%), glucuronic acid (10.0%), rhamnose (0.4%), glucose (22.5%), galactose (2.9%), xylose (6.0%), and fucose (1.1%).
32553965	3	54	theme	enzymatic	483:491	arg1	hydrolysis					493:502	the enzymatic hydrolysis	479:502	the enzymatic hydrolysis	479:502	The yield of reducing sugar and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate were used as indices to optimize the enzymatic hydrolysis of AAP, based on response surface methodology (RSM).
32553965	9	55	theme	antioxidant	1310:1320	arg1	activity					1322:1329	better antioxidant activity	1303:1329	better antioxidant activity	1303:1329	The antioxidant activity of the polysaccharide indicated that C-EAAP had better antioxidant activity than AAP.
32553965	4	56	theme	enzyme	619:624	arg1	dosage					626:631	enzyme dosage	619:631	enzyme dosage	619:631	The resulting optimal enzymolysis conditions were as follows: enzyme dosage, 13,500 U/g; enzymolysis temperature, 50 °C; and pH, 4.2.
32553965	4	56	theme	enzyme	619:624	arg1	13,500 U/g					634:643	13,500 U/g	634:643	13,500 U/g	634:643	The resulting optimal enzymolysis conditions were as follows: enzyme dosage, 13,500 U/g; enzymolysis temperature, 50 °C; and pH, 4.2.
32553965	4	56	theme	enzyme	619:624	arg1	pH					682:683	pH	682:683	pH	682:683	The resulting optimal enzymolysis conditions were as follows: enzyme dosage, 13,500 U/g; enzymolysis temperature, 50 °C; and pH, 4.2.
32553965	4	56	theme	enzyme	619:624	arg1	temperature					658:668	enzymolysis temperature	646:668	enzymolysis temperature	646:668	The resulting optimal enzymolysis conditions were as follows: enzyme dosage, 13,500 U/g; enzymolysis temperature, 50 °C; and pH, 4.2.
32553965	4	56	theme	enzyme	619:624	arg1	4.2					686:688	4.2	686:688	4.2	686:688	The resulting optimal enzymolysis conditions were as follows: enzyme dosage, 13,500 U/g; enzymolysis temperature, 50 °C; and pH, 4.2.
32553965	4	56	theme	enzyme	619:624	arg1	50 °C					671:675	50 °C	671:675	50 °C	671:675	The resulting optimal enzymolysis conditions were as follows: enzyme dosage, 13,500 U/g; enzymolysis temperature, 50 °C; and pH, 4.2.
32553965	10	57	theme	C-EAAP	1365:1370	arg1	rates					1356:1360	The scavenging rates	1341:1360	The scavenging rates of C-EAAP for hydroxyl radicals (·OH) and superoxide anion radicals (O2-·)	1341:1435	The scavenging rates of C-EAAP for hydroxyl radicals (·OH) and superoxide anion radicals (O2-·) were 1.65 and 1.90 times those of AAP.
32553965	10	57	theme	C-EAAP	1365:1370	arg1	times					1456:1460	1.65 and 1.90 times	1442:1460	1.65 and 1.90 times those of AAP	1442:1473	The scavenging rates of C-EAAP for hydroxyl radicals (·OH) and superoxide anion radicals (O2-·) were 1.65 and 1.90 times those of AAP.
32553965	6	58	theme	nucleic	948:954	arg1	acid					956:959	nucleic acid	948:959	nucleic acid	948:959	The degradation product of AAP (C-EAAP) was homogeneous and contained alpha and beta glycoside bonds, but did not contain protein or nucleic acid.
32553965	3	59	theme	response	521:528	arg1	RSM					551:553	RSM	551:553	RSM	551:553	The yield of reducing sugar and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate were used as indices to optimize the enzymatic hydrolysis of AAP, based on response surface methodology (RSM).
32553965	3	59	theme	response	521:528	arg1	methodology					538:548	response surface methodology	521:548	response surface methodology (RSM)	521:554	The yield of reducing sugar and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate were used as indices to optimize the enzymatic hydrolysis of AAP, based on response surface methodology (RSM).
32553965	1	60	theme	degradation	256:266	arg1	product					268:274	the degradation product	252:274	the degradation product of AAP	252:281	An efficient enzymatic hydrolysis method was developed and optimized for the degradation of auricularia auricula polysaccharide (AAP) and the degradation product of AAP was characterized.
34320760	2	0	theme	different	360:368	arg1	varieties					370:378	five different varieties	355:378	five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB)	355:430	Choline chloride based deep eutectic solvents (DESs) were used for pretreatment of five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB) and a comparative study of compositional and morphological changes was performed.
34320760	2	0	theme	different	360:368	arg1	bagasse					393:399	sugarcane bagasse	383:399	sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB)	383:430	Choline chloride based deep eutectic solvents (DESs) were used for pretreatment of five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB) and a comparative study of compositional and morphological changes was performed.
34320760	0	1	theme	sugarcane	83:91	arg1	sugarcane					83:91	sugarcane	83:91	sugarcane	83:91	Improving enzymatic digestibility of sugarcane bagasse from different varieties of sugarcane using deep eutectic solvent pretreatment.
34320760	0	1	theme	sugarcane	83:91	arg1	varieties					70:78	different varieties	60:78	different varieties of sugarcane	60:91	Improving enzymatic digestibility of sugarcane bagasse from different varieties of sugarcane using deep eutectic solvent pretreatment.
34320760	3	2	theme	glycerol	596:603	arg1	1:2					606:608	glycerol (1:2) and choline chloride	596:630	1:2	606:608	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	0	3	theme	eutectic	104:111	arg1	pretreatment					121:132	deep eutectic solvent pretreatment	99:132	deep eutectic solvent pretreatment	99:132	Improving enzymatic digestibility of sugarcane bagasse from different varieties of sugarcane using deep eutectic solvent pretreatment.
34320760	3	4	theme	lactic	633:638	arg1	acid					640:643	lactic acid	633:643	lactic acid (1:5)	633:649	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	3	4	theme	lactic	633:638	arg1	1:5					646:648	1:5	646:648	1:5	646:648	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	5	5	theme	highest	993:999	arg1	recovery					1001:1008	the highest recovery	989:1008	the highest recovery of 92.8% for choline chloride	989:1038	Glucose recovery was predominantly influenced by the glucose content, as SRA1 variety showed the highest recovery of 92.8% for choline chloride: glycerol DES pretreatment.
34320760	3	6	theme	eutectic	520:527	arg1	mixtures					529:536	Three eutectic mixtures	514:536	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5)	514:649	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	6	7	theme	DES	1098:1100	arg1	pretreatment					1102:1113	lactic acid DES pretreatment	1086:1113	lactic acid DES pretreatment	1086:1113	Choline chloride: lactic acid DES pretreatment demonstrated the most efficient lignin removal of 81.6% for ISB variety and the enzyme amenability was prominently increased to 98.5%.
34320760	3	8	used	used	656:659	arg2	mixtures					529:536	Three eutectic mixtures	514:536	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5)	514:649	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	0	9	theme	deep	99:102	arg1	pretreatment					121:132	deep eutectic solvent pretreatment	99:132	deep eutectic solvent pretreatment	99:132	Improving enzymatic digestibility of sugarcane bagasse from different varieties of sugarcane using deep eutectic solvent pretreatment.
34320760	5	10	theme	DES	1050:1052	arg1	pretreatment					1054:1065	glycerol DES pretreatment	1041:1065	glycerol DES pretreatment	1041:1065	Glucose recovery was predominantly influenced by the glucose content, as SRA1 variety showed the highest recovery of 92.8% for choline chloride: glycerol DES pretreatment.
34320760	6	11	theme	lignin	1147:1152	arg1	removal					1154:1160	the most efficient lignin removal	1128:1160	the most efficient lignin removal of 81.6% for ISB variety	1128:1185	Choline chloride: lactic acid DES pretreatment demonstrated the most efficient lignin removal of 81.6% for ISB variety and the enzyme amenability was prominently increased to 98.5%.
34320760	6	12	theme	%	1169:1169	arg1	removal					1154:1160	the most efficient lignin removal	1128:1160	the most efficient lignin removal of 81.6% for ISB variety	1128:1185	Choline chloride: lactic acid DES pretreatment demonstrated the most efficient lignin removal of 81.6% for ISB variety and the enzyme amenability was prominently increased to 98.5%.
34320760	2	13	theme	eutectic	300:307	arg1	DESs					319:322	DESs	319:322	DESs	319:322	Choline chloride based deep eutectic solvents (DESs) were used for pretreatment of five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB) and a comparative study of compositional and morphological changes was performed.
34320760	2	13	theme	eutectic	300:307	arg1	solvents					309:316	eutectic solvents	300:316	eutectic solvents (DESs)	300:323	Choline chloride based deep eutectic solvents (DESs) were used for pretreatment of five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB) and a comparative study of compositional and morphological changes was performed.
34320760	3	14	dep	mixtures	529:536	arg1	chloride					548:555	choline chloride	540:555	choline chloride	540:555	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	3	14	dep	mixtures	529:536	arg1	chloride					586:593	choline chloride	578:593	choline chloride	578:593	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	2	15	theme	changes	491:497	arg1	study					450:454	a comparative study	436:454	a comparative study of compositional and morphological changes	436:497	Choline chloride based deep eutectic solvents (DESs) were used for pretreatment of five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB) and a comparative study of compositional and morphological changes was performed.
34320760	5	16	theme	glucose	949:955	arg1	content					957:963	the glucose content	945:963	the glucose content	945:963	Glucose recovery was predominantly influenced by the glucose content, as SRA1 variety showed the highest recovery of 92.8% for choline chloride: glycerol DES pretreatment.
34320760	2	17	theme	bagasse	393:399	arg1	varieties					370:378	five different varieties	355:378	five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB)	355:430	Choline chloride based deep eutectic solvents (DESs) were used for pretreatment of five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB) and a comparative study of compositional and morphological changes was performed.
34320760	2	17	theme	bagasse	393:399	arg1	bagasse					393:399	sugarcane bagasse	383:399	sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB)	383:430	Choline chloride based deep eutectic solvents (DESs) were used for pretreatment of five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB) and a comparative study of compositional and morphological changes was performed.
34320760	5	18	theme	glycerol	1041:1048	arg1	pretreatment					1054:1065	glycerol DES pretreatment	1041:1065	glycerol DES pretreatment	1041:1065	Glucose recovery was predominantly influenced by the glucose content, as SRA1 variety showed the highest recovery of 92.8% for choline chloride: glycerol DES pretreatment.
34320760	0	19	theme	solvent	113:119	arg1	pretreatment					121:132	deep eutectic solvent pretreatment	99:132	deep eutectic solvent pretreatment	99:132	Improving enzymatic digestibility of sugarcane bagasse from different varieties of sugarcane using deep eutectic solvent pretreatment.
34320760	2	20	theme	comparative	438:448	arg1	study					450:454	a comparative study	436:454	a comparative study of compositional and morphological changes	436:497	Choline chloride based deep eutectic solvents (DESs) were used for pretreatment of five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB) and a comparative study of compositional and morphological changes was performed.
34320760	2	21	used	used	330:333	arg2	chloride					280:287	Choline chloride	272:287	Choline chloride based deep eutectic solvents (DESs)	272:323	Choline chloride based deep eutectic solvents (DESs) were used for pretreatment of five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB) and a comparative study of compositional and morphological changes was performed.
34320760	1	22	theme	sugar	186:190	arg1	industry					192:199	the sugar industry	182:199	the sugar industry	182:199	Sugarcane bagasse, a fundamental by-product of the sugar industry, was utilised to improve its digestibility for bioenergy applications.
34320760	0	23	theme	enzymatic	10:18	arg1	digestibility					20:32	enzymatic digestibility	10:32	enzymatic digestibility of sugarcane bagasse from different varieties of sugarcane	10:91	Improving enzymatic digestibility of sugarcane bagasse from different varieties of sugarcane using deep eutectic solvent pretreatment.
34320760	4	24	theme	XRD	804:806	arg1	analysis					808:815	XRD analysis	804:815	XRD analysis	804:815	Physico-chemical characterizations performed using FE-SEM, FTIR, TGA and XRD analysis consistently indicated disruption of bagasse structure after DES pretreatment.
34320760	1	25	theme	industry	192:199	arg1	bagasse					145:151	Sugarcane bagasse	135:151	Sugarcane bagasse	135:151	Sugarcane bagasse, a fundamental by-product of the sugar industry, was utilised to improve its digestibility for bioenergy applications.
34320760	1	25	theme	industry	192:199	arg1	by-product					168:177	a fundamental by-product	154:177	a fundamental by-product of the sugar industry	154:199	Sugarcane bagasse, a fundamental by-product of the sugar industry, was utilised to improve its digestibility for bioenergy applications.
34320760	6	26	theme	enzyme	1195:1200	arg1	amenability					1202:1212	the enzyme amenability	1191:1212	the enzyme amenability	1191:1212	Choline chloride: lactic acid DES pretreatment demonstrated the most efficient lignin removal of 81.6% for ISB variety and the enzyme amenability was prominently increased to 98.5%.
34320760	2	27	theme	sugarcane	383:391	arg1	bagasse					393:399	sugarcane bagasse	383:399	sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB)	383:430	Choline chloride based deep eutectic solvents (DESs) were used for pretreatment of five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB) and a comparative study of compositional and morphological changes was performed.
34320760	5	28	theme	Glucose	896:902	arg1	recovery					904:911	Glucose recovery	896:911	Glucose recovery	896:911	Glucose recovery was predominantly influenced by the glucose content, as SRA1 variety showed the highest recovery of 92.8% for choline chloride: glycerol DES pretreatment.
34320760	4	29	theme	DES	878:880	arg1	pretreatment					882:893	DES pretreatment	878:893	DES pretreatment	878:893	Physico-chemical characterizations performed using FE-SEM, FTIR, TGA and XRD analysis consistently indicated disruption of bagasse structure after DES pretreatment.
34320760	5	30	theme	choline	1023:1029	arg1	chloride					1031:1038	choline chloride	1023:1038	choline chloride	1023:1038	Glucose recovery was predominantly influenced by the glucose content, as SRA1 variety showed the highest recovery of 92.8% for choline chloride: glycerol DES pretreatment.
34320760	3	31	theme	choline	540:546	arg1	chloride					548:555	choline chloride	540:555	choline chloride	540:555	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	3	31	theme	choline	540:546	arg1	chloride					586:593	choline chloride	578:593	choline chloride	578:593	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	0	32	theme	bagasse	47:53	arg1	digestibility					20:32	enzymatic digestibility	10:32	enzymatic digestibility of sugarcane bagasse from different varieties of sugarcane	10:91	Improving enzymatic digestibility of sugarcane bagasse from different varieties of sugarcane using deep eutectic solvent pretreatment.
34320760	6	33	theme	lactic	1086:1091	arg1	pretreatment					1102:1113	lactic acid DES pretreatment	1086:1113	lactic acid DES pretreatment	1086:1113	Choline chloride: lactic acid DES pretreatment demonstrated the most efficient lignin removal of 81.6% for ISB variety and the enzyme amenability was prominently increased to 98.5%.
34320760	0	34	theme	sugarcane	37:45	arg1	bagasse					47:53	sugarcane bagasse	37:53	sugarcane bagasse	37:53	Improving enzymatic digestibility of sugarcane bagasse from different varieties of sugarcane using deep eutectic solvent pretreatment.
34320760	3	35	theme	saccharification	702:717	arg1	efficiency					719:728	saccharification efficiency	702:728	saccharification efficiency	702:728	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	2	36	theme	morphological	477:489	arg1	changes					491:497	compositional and morphological changes	459:497	changes	491:497	Choline chloride based deep eutectic solvents (DESs) were used for pretreatment of five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB) and a comparative study of compositional and morphological changes was performed.
34320760	0	37	theme	different	60:68	arg1	sugarcane					83:91	sugarcane	83:91	sugarcane	83:91	Improving enzymatic digestibility of sugarcane bagasse from different varieties of sugarcane using deep eutectic solvent pretreatment.
34320760	0	37	theme	different	60:68	arg1	varieties					70:78	different varieties	60:78	different varieties of sugarcane	60:91	Improving enzymatic digestibility of sugarcane bagasse from different varieties of sugarcane using deep eutectic solvent pretreatment.
34320760	4	38	theme	bagasse	854:860	arg1	structure					862:870	bagasse structure	854:870	bagasse structure	854:870	Physico-chemical characterizations performed using FE-SEM, FTIR, TGA and XRD analysis consistently indicated disruption of bagasse structure after DES pretreatment.
34320760	0	39	from	varieties	70:78	arg1	digestibility					20:32	enzymatic digestibility	10:32	enzymatic digestibility of sugarcane bagasse from different varieties of sugarcane	10:91	Improving enzymatic digestibility of sugarcane bagasse from different varieties of sugarcane using deep eutectic solvent pretreatment.
34320760	3	40	theme	malonic	558:564	arg1	1:1					572:574	1:1	572:574	1:1	572:574	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	3	40	theme	malonic	558:564	arg1	acid					566:569	malonic acid	558:569	malonic acid (1:1)	558:575	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	6	41	theme	acid	1093:1096	arg1	pretreatment					1102:1113	lactic acid DES pretreatment	1086:1113	lactic acid DES pretreatment	1086:1113	Choline chloride: lactic acid DES pretreatment demonstrated the most efficient lignin removal of 81.6% for ISB variety and the enzyme amenability was prominently increased to 98.5%.
34320760	3	42	theme	choline	615:621	arg1	chloride					623:630	glycerol (1:2) and choline chloride	596:630	chloride	623:630	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	6	43	theme	ISB	1175:1177	arg1	variety					1179:1185	ISB variety	1175:1185	ISB variety	1175:1185	Choline chloride: lactic acid DES pretreatment demonstrated the most efficient lignin removal of 81.6% for ISB variety and the enzyme amenability was prominently increased to 98.5%.
34320760	1	44	theme	Sugarcane	135:143	arg1	bagasse					145:151	Sugarcane bagasse	135:151	Sugarcane bagasse	135:151	Sugarcane bagasse, a fundamental by-product of the sugar industry, was utilised to improve its digestibility for bioenergy applications.
34320760	1	44	theme	Sugarcane	135:143	arg1	by-product					168:177	a fundamental by-product	154:177	a fundamental by-product of the sugar industry	154:199	Sugarcane bagasse, a fundamental by-product of the sugar industry, was utilised to improve its digestibility for bioenergy applications.
34320760	3	45	theme	choline	578:584	arg1	chloride					548:555	choline chloride	540:555	choline chloride	540:555	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	3	45	theme	choline	578:584	arg1	chloride					586:593	choline chloride	578:593	choline chloride	578:593	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	2	46	dep	bagasse	393:399	arg1	ISB					427:429	ISB	427:429	ISB	427:429	Choline chloride based deep eutectic solvents (DESs) were used for pretreatment of five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB) and a comparative study of compositional and morphological changes was performed.
34320760	2	46	dep	bagasse	393:399	arg1	SRA1					402:405	SRA1	402:405	SRA1	402:405	Choline chloride based deep eutectic solvents (DESs) were used for pretreatment of five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB) and a comparative study of compositional and morphological changes was performed.
34320760	2	46	dep	bagasse	393:399	arg1	MA239					420:424	MA239	420:424	MA239	420:424	Choline chloride based deep eutectic solvents (DESs) were used for pretreatment of five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB) and a comparative study of compositional and morphological changes was performed.
34320760	2	46	dep	bagasse	393:399	arg1	Q208					414:417	Q208	414:417	Q208	414:417	Choline chloride based deep eutectic solvents (DESs) were used for pretreatment of five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB) and a comparative study of compositional and morphological changes was performed.
34320760	3	47	dep	chloride	548:555	arg1	1:1					572:574	1:1	572:574	1:1	572:574	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	3	47	dep	chloride	548:555	arg1	1:2					606:608	glycerol (1:2) and choline chloride	596:630	1:2	606:608	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	3	47	dep	chloride	548:555	arg1	acid					640:643	lactic acid	633:643	lactic acid (1:5)	633:649	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	3	47	dep	chloride	548:555	arg1	acid					566:569	malonic acid	558:569	malonic acid (1:1)	558:575	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	3	47	dep	chloride	548:555	arg1	1:5					646:648	1:5	646:648	1:5	646:648	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	3	47	dep	chloride	548:555	arg1	chloride					623:630	glycerol (1:2) and choline chloride	596:630	chloride	623:630	Three eutectic mixtures - choline chloride: malonic acid (1:1), choline chloride: glycerol (1:2) and choline chloride: lactic acid (1:5) were used to selectively remove lignin and improve saccharification efficiency.
34320760	4	48	theme	structure	862:870	arg1	disruption					840:849	disruption	840:849	disruption of bagasse structure	840:870	Physico-chemical characterizations performed using FE-SEM, FTIR, TGA and XRD analysis consistently indicated disruption of bagasse structure after DES pretreatment.
34320760	5	49	theme	%	1017:1017	arg1	recovery					1001:1008	the highest recovery	989:1008	the highest recovery of 92.8% for choline chloride	989:1038	Glucose recovery was predominantly influenced by the glucose content, as SRA1 variety showed the highest recovery of 92.8% for choline chloride: glycerol DES pretreatment.
34320760	2	50	theme	Choline	272:278	arg1	chloride					280:287	Choline chloride	272:287	Choline chloride based deep eutectic solvents (DESs)	272:323	Choline chloride based deep eutectic solvents (DESs) were used for pretreatment of five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB) and a comparative study of compositional and morphological changes was performed.
34320760	1	51	theme	fundamental	156:166	arg1	bagasse					145:151	Sugarcane bagasse	135:151	Sugarcane bagasse	135:151	Sugarcane bagasse, a fundamental by-product of the sugar industry, was utilised to improve its digestibility for bioenergy applications.
34320760	1	51	theme	fundamental	156:166	arg1	by-product					168:177	a fundamental by-product	154:177	a fundamental by-product of the sugar industry	154:199	Sugarcane bagasse, a fundamental by-product of the sugar industry, was utilised to improve its digestibility for bioenergy applications.
34320760	4	52	theme	Physico-chemical	731:746	arg1	characterizations					748:764	Physico-chemical characterizations	731:764	Physico-chemical characterizations performed using FE-SEM, FTIR, TGA and XRD analysis	731:815	Physico-chemical characterizations performed using FE-SEM, FTIR, TGA and XRD analysis consistently indicated disruption of bagasse structure after DES pretreatment.
34320760	5	53	theme	SRA1	969:972	arg1	variety					974:980	SRA1 variety	969:980	SRA1 variety	969:980	Glucose recovery was predominantly influenced by the glucose content, as SRA1 variety showed the highest recovery of 92.8% for choline chloride: glycerol DES pretreatment.
34320760	6	54	theme	Choline	1068:1074	arg1	chloride					1076:1083	Choline chloride	1068:1083	Choline chloride: lactic acid DES pretreatment demonstrated the most efficient lignin removal of 81.6% for ISB variety and the enzyme amenability was prominently increased to 98.5%.	1068:1248	Choline chloride: lactic acid DES pretreatment demonstrated the most efficient lignin removal of 81.6% for ISB variety and the enzyme amenability was prominently increased to 98.5%.
34320760	6	55	theme	efficient	1137:1145	arg1	removal					1154:1160	the most efficient lignin removal	1128:1160	the most efficient lignin removal of 81.6% for ISB variety	1128:1185	Choline chloride: lactic acid DES pretreatment demonstrated the most efficient lignin removal of 81.6% for ISB variety and the enzyme amenability was prominently increased to 98.5%.
34320760	2	56	theme	compositional	459:471	arg1	changes					491:497	compositional and morphological changes	459:497	changes	491:497	Choline chloride based deep eutectic solvents (DESs) were used for pretreatment of five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB) and a comparative study of compositional and morphological changes was performed.
34320760	6	57	dep	chloride	1076:1083	arg1	demonstrated					1115:1126	demonstrated	1115:1126	demonstrated the most efficient lignin removal of 81.6% for ISB variety	1115:1185	Choline chloride: lactic acid DES pretreatment demonstrated the most efficient lignin removal of 81.6% for ISB variety and the enzyme amenability was prominently increased to 98.5%.
34320760	6	57	dep	chloride	1076:1083	arg1	increased					1230:1238	increased	1230:1238	was prominently increased to 98.5%	1214:1247	Choline chloride: lactic acid DES pretreatment demonstrated the most efficient lignin removal of 81.6% for ISB variety and the enzyme amenability was prominently increased to 98.5%.
34320760	2	58	theme	varieties	370:378	arg1	pretreatment					339:350	pretreatment	339:350	pretreatment of five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB)	339:430	Choline chloride based deep eutectic solvents (DESs) were used for pretreatment of five different varieties of sugarcane bagasse (SRA1, SRA5, Q208, MA239, ISB) and a comparative study of compositional and morphological changes was performed.
34320760	1	59	theme	bioenergy	248:256	arg1	applications					258:269	bioenergy applications	248:269	bioenergy applications	248:269	Sugarcane bagasse, a fundamental by-product of the sugar industry, was utilised to improve its digestibility for bioenergy applications.
32198034	0	0	theme	wound	92:96	arg1	healing					98:104	diabetic wound healing	83:104	diabetic wound healing	83:104	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	0	0	theme	wound	92:96	arg1	derivatives					38:48	Hyperbranched cationic polysaccharide derivatives	0:48	Hyperbranched cationic polysaccharide derivatives	0:48	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	8	1	theme	wound	998:1002	arg1	healing					1004:1010	wound healing	998:1010	wound healing	998:1010	Wound models in diabetic rats demonstrated that treatment of G-EDA/siMMP-9 could potently knock down MMP-9 of skin wound tissues and then enhanced wound healing.
32198034	8	2	theme	wound	966:970	arg1	tissues					972:978	skin wound tissues	961:978	skin wound tissues	961:978	Wound models in diabetic rats demonstrated that treatment of G-EDA/siMMP-9 could potently knock down MMP-9 of skin wound tissues and then enhanced wound healing.
32198034	5	3	theme	negligible	602:611	arg1	cytotoxicity					613:624	negligible cytotoxicity	602:624	negligible cytotoxicity	602:624	All HCP/siRNA exhibited negligible cytotoxicity.
32198034	8	4	theme	skin	961:964	arg1	tissues					972:978	skin wound tissues	961:978	skin wound tissues	961:978	Wound models in diabetic rats demonstrated that treatment of G-EDA/siMMP-9 could potently knock down MMP-9 of skin wound tissues and then enhanced wound healing.
32198034	7	5	theme	HaCaT	838:842	arg1	cells					844:848	HaCaT cells	838:848	HaCaT cells	838:848	The delivery of MMP-9 siRNA (siMMP-9) by G-EDA and G-DETA significantly inhibited MMP-9 in HaCaT cells.
32198034	6	6	theme	more	693:696	arg1	siRNA					698:702	much more siRNA	688:702	much more siRNA	688:702	Compared with A-EDA and A-DETA, G-EDA and G-DETA could carry much more siRNA into cells and then escape from endosomes.
32198034	0	7	theme	cationic	14:21	arg1	derivatives					38:48	Hyperbranched cationic polysaccharide derivatives	0:48	Hyperbranched cationic polysaccharide derivatives	0:48	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	0	7	theme	cationic	14:21	arg1	healing					98:104	diabetic wound healing	83:104	diabetic wound healing	83:104	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	0	7	theme	cationic	14:21	arg1	delivery					70:77	efficient siRNA delivery	54:77	efficient siRNA delivery	54:77	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	8	8	theme	tissues	972:978	arg1	MMP-9					952:956	down MMP-9	947:956	down MMP-9 of skin wound tissues	947:978	Wound models in diabetic rats demonstrated that treatment of G-EDA/siMMP-9 could potently knock down MMP-9 of skin wound tissues and then enhanced wound healing.
32198034	8	9	theme	Wound	851:855	arg1	models					857:862	Wound models	851:862	Wound models in diabetic rats	851:879	Wound models in diabetic rats demonstrated that treatment of G-EDA/siMMP-9 could potently knock down MMP-9 of skin wound tissues and then enhanced wound healing.
32198034	0	10	theme	Hyperbranched	0:12	arg1	derivatives					38:48	Hyperbranched cationic polysaccharide derivatives	0:48	Hyperbranched cationic polysaccharide derivatives	0:48	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	0	10	theme	Hyperbranched	0:12	arg1	healing					98:104	diabetic wound healing	83:104	diabetic wound healing	83:104	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	0	10	theme	Hyperbranched	0:12	arg1	delivery					70:77	efficient siRNA delivery	54:77	efficient siRNA delivery	54:77	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	3	11	dep	efficiency	427:436	arg1	The					423:425	The	423:425	The	423:425	The efficiency and safety of these HCPs to deliver siRNA were explored in vitro and in vivo.
32198034	8	12	theme	diabetic	867:874	arg1	rats					876:879	diabetic rats	867:879	diabetic rats	867:879	Wound models in diabetic rats demonstrated that treatment of G-EDA/siMMP-9 could potently knock down MMP-9 of skin wound tissues and then enhanced wound healing.
32198034	1	13	theme	Gene	119:122	arg1	vectors					124:130	Gene vectors	119:130	Gene vectors	119:130	Gene vectors are important for successful siRNA delivery.
32198034	8	14	from	models	857:862	arg1	rats					876:879	diabetic rats	867:879	diabetic rats	867:879	Wound models in diabetic rats demonstrated that treatment of G-EDA/siMMP-9 could potently knock down MMP-9 of skin wound tissues and then enhanced wound healing.
32198034	0	15	theme	polysaccharide	23:36	arg1	derivatives					38:48	Hyperbranched cationic polysaccharide derivatives	0:48	Hyperbranched cationic polysaccharide derivatives	0:48	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	0	15	theme	polysaccharide	23:36	arg1	healing					98:104	diabetic wound healing	83:104	diabetic wound healing	83:104	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	0	15	theme	polysaccharide	23:36	arg1	delivery					70:77	efficient siRNA delivery	54:77	efficient siRNA delivery	54:77	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	2	16	theme	cationic	205:212	arg1	derivatives					229:239	hyperbranched cationic polysaccharide derivatives	191:239	hyperbranched cationic polysaccharide derivatives (HCP)	191:245	Four types of hyperbranched cationic polysaccharide derivatives (HCP) were synthesized by conjuncting 1,2-ethylenediamine (EDA) and diethylenetriamine (DETA) with glycogen or amylopectin respectively and named as G-EDA, G-DETA, A-EDA and A-DETA.
32198034	2	16	theme	cationic	205:212	arg1	HCP					242:244	HCP	242:244	HCP	242:244	Four types of hyperbranched cationic polysaccharide derivatives (HCP) were synthesized by conjuncting 1,2-ethylenediamine (EDA) and diethylenetriamine (DETA) with glycogen or amylopectin respectively and named as G-EDA, G-DETA, A-EDA and A-DETA.
32198034	9	17	theme	safe	1062:1065	arg1	approach					1067:1074	an effective and safe approach	1045:1074	an effective and safe approach for siRNA delivery	1045:1093	In summary, this study provided an effective and safe approach for siRNA delivery in vitro and in vivo.
32198034	0	18	theme	efficient	54:62	arg1	derivatives					38:48	Hyperbranched cationic polysaccharide derivatives	0:48	Hyperbranched cationic polysaccharide derivatives	0:48	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	0	18	theme	efficient	54:62	arg1	delivery					70:77	efficient siRNA delivery	54:77	efficient siRNA delivery	54:77	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	2	19	theme	hyperbranched	191:203	arg1	derivatives					229:239	hyperbranched cationic polysaccharide derivatives	191:239	hyperbranched cationic polysaccharide derivatives (HCP)	191:245	Four types of hyperbranched cationic polysaccharide derivatives (HCP) were synthesized by conjuncting 1,2-ethylenediamine (EDA) and diethylenetriamine (DETA) with glycogen or amylopectin respectively and named as G-EDA, G-DETA, A-EDA and A-DETA.
32198034	2	19	theme	hyperbranched	191:203	arg1	HCP					242:244	HCP	242:244	HCP	242:244	Four types of hyperbranched cationic polysaccharide derivatives (HCP) were synthesized by conjuncting 1,2-ethylenediamine (EDA) and diethylenetriamine (DETA) with glycogen or amylopectin respectively and named as G-EDA, G-DETA, A-EDA and A-DETA.
32198034	0	20	dep	delivery	70:77	arg1	enhancement					106:116	enhancement	106:116	enhancement	106:116	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	9	21	theme	siRNA	1080:1084	arg1	delivery					1086:1093	siRNA delivery	1080:1093	siRNA delivery	1080:1093	In summary, this study provided an effective and safe approach for siRNA delivery in vitro and in vivo.
32198034	3	22	theme	HCPs	458:461	arg1	safety					442:447	safety	442:447	safety	442:447	The efficiency and safety of these HCPs to deliver siRNA were explored in vitro and in vivo.
32198034	3	22	theme	HCPs	458:461	arg1	efficiency					427:436	efficiency	427:436	efficiency	427:436	The efficiency and safety of these HCPs to deliver siRNA were explored in vitro and in vivo.
32198034	2	23	with	1,2-ethylenediamine	279:297	arg1	amylopectin					352:362	amylopectin	352:362	amylopectin	352:362	Four types of hyperbranched cationic polysaccharide derivatives (HCP) were synthesized by conjuncting 1,2-ethylenediamine (EDA) and diethylenetriamine (DETA) with glycogen or amylopectin respectively and named as G-EDA, G-DETA, A-EDA and A-DETA.
32198034	2	23	with	1,2-ethylenediamine	279:297	arg1	glycogen					340:347	glycogen	340:347	glycogen	340:347	Four types of hyperbranched cationic polysaccharide derivatives (HCP) were synthesized by conjuncting 1,2-ethylenediamine (EDA) and diethylenetriamine (DETA) with glycogen or amylopectin respectively and named as G-EDA, G-DETA, A-EDA and A-DETA.
32198034	7	24	theme	MMP-9	763:767	arg1	siMMP-9					776:782	siMMP-9	776:782	siMMP-9	776:782	The delivery of MMP-9 siRNA (siMMP-9) by G-EDA and G-DETA significantly inhibited MMP-9 in HaCaT cells.
32198034	7	24	theme	MMP-9	763:767	arg1	siRNA					769:773	MMP-9 siRNA	763:773	MMP-9 siRNA (siMMP-9)	763:783	The delivery of MMP-9 siRNA (siMMP-9) by G-EDA and G-DETA significantly inhibited MMP-9 in HaCaT cells.
32198034	0	25	theme	siRNA	64:68	arg1	derivatives					38:48	Hyperbranched cationic polysaccharide derivatives	0:48	Hyperbranched cationic polysaccharide derivatives	0:48	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	0	25	theme	siRNA	64:68	arg1	delivery					70:77	efficient siRNA delivery	54:77	efficient siRNA delivery	54:77	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	8	26	theme	G-EDA/siMMP-9	912:924	arg1	treatment					899:907	treatment	899:907	treatment of G-EDA/siMMP-9	899:924	Wound models in diabetic rats demonstrated that treatment of G-EDA/siMMP-9 could potently knock down MMP-9 of skin wound tissues and then enhanced wound healing.
32198034	0	27	dep	derivatives	38:48	arg1	healing					98:104	diabetic wound healing	83:104	diabetic wound healing	83:104	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	0	27	dep	derivatives	38:48	arg1	derivatives					38:48	Hyperbranched cationic polysaccharide derivatives	0:48	Hyperbranched cationic polysaccharide derivatives	0:48	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	0	27	dep	derivatives	38:48	arg1	delivery					70:77	efficient siRNA delivery	54:77	efficient siRNA delivery	54:77	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	2	28	theme	derivatives	229:239	arg1	A-EDA					405:409	A-EDA	405:409	A-EDA	405:409	Four types of hyperbranched cationic polysaccharide derivatives (HCP) were synthesized by conjuncting 1,2-ethylenediamine (EDA) and diethylenetriamine (DETA) with glycogen or amylopectin respectively and named as G-EDA, G-DETA, A-EDA and A-DETA.
32198034	2	28	theme	derivatives	229:239	arg1	types					182:186	Four types	177:186	Four types of hyperbranched cationic polysaccharide derivatives (HCP)	177:245	Four types of hyperbranched cationic polysaccharide derivatives (HCP) were synthesized by conjuncting 1,2-ethylenediamine (EDA) and diethylenetriamine (DETA) with glycogen or amylopectin respectively and named as G-EDA, G-DETA, A-EDA and A-DETA.
32198034	2	28	theme	derivatives	229:239	arg1	G-DETA					397:402	G-DETA	397:402	G-DETA	397:402	Four types of hyperbranched cationic polysaccharide derivatives (HCP) were synthesized by conjuncting 1,2-ethylenediamine (EDA) and diethylenetriamine (DETA) with glycogen or amylopectin respectively and named as G-EDA, G-DETA, A-EDA and A-DETA.
32198034	2	28	theme	derivatives	229:239	arg1	A-DETA					415:420	A-DETA	415:420	A-DETA	415:420	Four types of hyperbranched cationic polysaccharide derivatives (HCP) were synthesized by conjuncting 1,2-ethylenediamine (EDA) and diethylenetriamine (DETA) with glycogen or amylopectin respectively and named as G-EDA, G-DETA, A-EDA and A-DETA.
32198034	7	29	from	MMP-9	829:833	arg1	cells					844:848	HaCaT cells	838:848	HaCaT cells	838:848	The delivery of MMP-9 siRNA (siMMP-9) by G-EDA and G-DETA significantly inhibited MMP-9 in HaCaT cells.
32198034	1	30	theme	successful	150:159	arg1	delivery					167:174	successful siRNA delivery	150:174	successful siRNA delivery	150:174	Gene vectors are important for successful siRNA delivery.
32198034	9	31	theme	effective	1048:1056	arg1	approach					1067:1074	an effective and safe approach	1045:1074	an effective and safe approach for siRNA delivery	1045:1093	In summary, this study provided an effective and safe approach for siRNA delivery in vitro and in vivo.
32198034	0	32	theme	diabetic	83:90	arg1	healing					98:104	diabetic wound healing	83:104	diabetic wound healing	83:104	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	0	32	theme	diabetic	83:90	arg1	derivatives					38:48	Hyperbranched cationic polysaccharide derivatives	0:48	Hyperbranched cationic polysaccharide derivatives	0:48	Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.
32198034	2	33	theme	polysaccharide	214:227	arg1	derivatives					229:239	hyperbranched cationic polysaccharide derivatives	191:239	hyperbranched cationic polysaccharide derivatives (HCP)	191:245	Four types of hyperbranched cationic polysaccharide derivatives (HCP) were synthesized by conjuncting 1,2-ethylenediamine (EDA) and diethylenetriamine (DETA) with glycogen or amylopectin respectively and named as G-EDA, G-DETA, A-EDA and A-DETA.
32198034	2	33	theme	polysaccharide	214:227	arg1	HCP					242:244	HCP	242:244	HCP	242:244	Four types of hyperbranched cationic polysaccharide derivatives (HCP) were synthesized by conjuncting 1,2-ethylenediamine (EDA) and diethylenetriamine (DETA) with glycogen or amylopectin respectively and named as G-EDA, G-DETA, A-EDA and A-DETA.
32198034	1	34	theme	siRNA	161:165	arg1	delivery					167:174	successful siRNA delivery	150:174	successful siRNA delivery	150:174	Gene vectors are important for successful siRNA delivery.
32198034	6	35	contain	carry	682:686	arg2	siRNA					698:702	much more siRNA	688:702	much more siRNA	688:702	Compared with A-EDA and A-DETA, G-EDA and G-DETA could carry much more siRNA into cells and then escape from endosomes.
32198034	6	35	contain	carry	682:686	arg1	G-DETA					669:674	G-DETA	669:674	G-DETA	669:674	Compared with A-EDA and A-DETA, G-EDA and G-DETA could carry much more siRNA into cells and then escape from endosomes.
32198034	6	35	contain	carry	682:686	arg1	G-EDA					659:663	G-EDA	659:663	G-EDA	659:663	Compared with A-EDA and A-DETA, G-EDA and G-DETA could carry much more siRNA into cells and then escape from endosomes.
32198034	8	36	theme	down	947:950	arg1	MMP-9					952:956	down MMP-9	947:956	down MMP-9 of skin wound tissues	947:978	Wound models in diabetic rats demonstrated that treatment of G-EDA/siMMP-9 could potently knock down MMP-9 of skin wound tissues and then enhanced wound healing.
32198034	7	37	theme	siRNA	769:773	arg1	delivery					751:758	The delivery	747:758	The delivery of MMP-9 siRNA (siMMP-9) by G-EDA and G-DETA	747:803	The delivery of MMP-9 siRNA (siMMP-9) by G-EDA and G-DETA significantly inhibited MMP-9 in HaCaT cells.
33328537	0	0	theme	milk	86:89	arg1	metagenome					91:100	the milk metagenome	82:100	the milk metagenome	82:100	Maternal diet alters human milk oligosaccharide composition with implications for the milk metagenome.
33328537	3	1	from	effect	522:527	arg1	diet					538:541	maternal diet	529:541	maternal diet	529:541	Hypothesizing that the maternal diet itself might influence HMO composition, we sought to directly determine the effect maternal diet on HMO and the milk bacteria.
33328537	6	2	theme	HMO	1060:1062	arg1	composition					1064:1074	milk HMO composition	1055:1074	milk HMO composition	1055:1074	These studies reveal a successive mechanism by which the maternal diet during lactation alters milk HMO composition, which in turn shapes the functional milk microbiome prior to infant ingestion.
33328537	4	3	theme	energy	679:684	arg1	sources					686:692	distinct maternal dietary carbohydrate and energy sources	636:692	sources	686:692	Employing a human cross-over study design, we demonstrate that distinct maternal dietary carbohydrate and energy sources preferentially alter milk concentrations of HMO, including fucosylated species.
33328537	3	4	theme	maternal	529:536	arg1	diet					538:541	maternal diet	529:541	maternal diet	529:541	Hypothesizing that the maternal diet itself might influence HMO composition, we sought to directly determine the effect maternal diet on HMO and the milk bacteria.
33328537	4	5	theme	fucosylated	753:763	arg1	species					765:771	fucosylated species	753:771	fucosylated species	753:771	Employing a human cross-over study design, we demonstrate that distinct maternal dietary carbohydrate and energy sources preferentially alter milk concentrations of HMO, including fucosylated species.
33328537	3	6	dep	diet	538:541	arg1	HMO					546:548	HMO	546:548	HMO	546:548	Hypothesizing that the maternal diet itself might influence HMO composition, we sought to directly determine the effect maternal diet on HMO and the milk bacteria.
33328537	3	6	dep	diet	538:541	arg1	bacteria					563:570	the milk bacteria	554:570	the milk bacteria	554:570	Hypothesizing that the maternal diet itself might influence HMO composition, we sought to directly determine the effect maternal diet on HMO and the milk bacteria.
33328537	4	7	theme	distinct	636:643	arg1	carbohydrate					662:673	distinct maternal dietary carbohydrate and energy sources	636:692	carbohydrate	662:673	Employing a human cross-over study design, we demonstrate that distinct maternal dietary carbohydrate and energy sources preferentially alter milk concentrations of HMO, including fucosylated species.
33328537	6	8	theme	successive	983:992	arg1	mechanism					994:1002	a successive mechanism	981:1002	a successive mechanism by which the maternal diet during lactation alters milk HMO composition, which in turn shapes the functional milk microbiome prior to infant ingestion	981:1153	These studies reveal a successive mechanism by which the maternal diet during lactation alters milk HMO composition, which in turn shapes the functional milk microbiome prior to infant ingestion.
33328537	2	9	theme	milk	261:264	arg1	HMO					284:286	HMO	284:286	HMO	284:286	Human milk oligosaccharides (HMO) are favorable macromolecules which are, interestingly, indigestible by the infant but serve as substrates for bacteria.
33328537	2	9	theme	milk	261:264	arg1	oligosaccharides					266:281	Human milk oligosaccharides	255:281	Human milk oligosaccharides (HMO)	255:287	Human milk oligosaccharides (HMO) are favorable macromolecules which are, interestingly, indigestible by the infant but serve as substrates for bacteria.
33328537	2	9	theme	milk	261:264	arg1	macromolecules					303:316	favorable macromolecules	293:316	favorable macromolecules which are, interestingly, indigestible by the infant but serve as substrates for bacteria	293:406	Human milk oligosaccharides (HMO) are favorable macromolecules which are, interestingly, indigestible by the infant but serve as substrates for bacteria.
33328537	3	10	theme	maternal	432:439	arg1	diet					441:444	the maternal diet itself	428:451	the maternal diet itself	428:451	Hypothesizing that the maternal diet itself might influence HMO composition, we sought to directly determine the effect maternal diet on HMO and the milk bacteria.
33328537	4	11	theme	HMO	738:740	arg1	species					765:771	fucosylated species	753:771	fucosylated species	753:771	Employing a human cross-over study design, we demonstrate that distinct maternal dietary carbohydrate and energy sources preferentially alter milk concentrations of HMO, including fucosylated species.
33328537	4	11	theme	HMO	738:740	arg1	concentrations					720:733	milk concentrations	715:733	milk concentrations	715:733	Employing a human cross-over study design, we demonstrate that distinct maternal dietary carbohydrate and energy sources preferentially alter milk concentrations of HMO, including fucosylated species.
33328537	4	12	theme	dietary	654:660	arg1	carbohydrate					662:673	distinct maternal dietary carbohydrate and energy sources	636:692	carbohydrate	662:673	Employing a human cross-over study design, we demonstrate that distinct maternal dietary carbohydrate and energy sources preferentially alter milk concentrations of HMO, including fucosylated species.
33328537	0	13	theme	Maternal	0:7	arg1	diet					9:12	Maternal diet	0:12	Maternal diet	0:12	Maternal diet alters human milk oligosaccharide composition with implications for the milk metagenome.
33328537	4	14	theme	milk	715:718	arg1	species					765:771	fucosylated species	753:771	fucosylated species	753:771	Employing a human cross-over study design, we demonstrate that distinct maternal dietary carbohydrate and energy sources preferentially alter milk concentrations of HMO, including fucosylated species.
33328537	4	14	theme	milk	715:718	arg1	concentrations					720:733	milk concentrations	715:733	milk concentrations	715:733	Employing a human cross-over study design, we demonstrate that distinct maternal dietary carbohydrate and energy sources preferentially alter milk concentrations of HMO, including fucosylated species.
33328537	2	15	theme	favorable	293:301	arg1	oligosaccharides					266:281	Human milk oligosaccharides	255:281	Human milk oligosaccharides (HMO)	255:287	Human milk oligosaccharides (HMO) are favorable macromolecules which are, interestingly, indigestible by the infant but serve as substrates for bacteria.
33328537	2	15	theme	favorable	293:301	arg1	substrates					384:393	substrates	384:393	substrates for bacteria	384:406	Human milk oligosaccharides (HMO) are favorable macromolecules which are, interestingly, indigestible by the infant but serve as substrates for bacteria.
33328537	2	15	theme	favorable	293:301	arg1	macromolecules					303:316	favorable macromolecules	293:316	favorable macromolecules which are, interestingly, indigestible by the infant but serve as substrates for bacteria	293:406	Human milk oligosaccharides (HMO) are favorable macromolecules which are, interestingly, indigestible by the infant but serve as substrates for bacteria.
33328537	4	16	theme	maternal	645:652	arg1	carbohydrate					662:673	distinct maternal dietary carbohydrate and energy sources	636:692	carbohydrate	662:673	Employing a human cross-over study design, we demonstrate that distinct maternal dietary carbohydrate and energy sources preferentially alter milk concentrations of HMO, including fucosylated species.
33328537	0	17	theme	human	21:25	arg1	composition					48:58	human milk oligosaccharide composition	21:58	human milk oligosaccharide composition	21:58	Maternal diet alters human milk oligosaccharide composition with implications for the milk metagenome.
33328537	1	18	theme	Human	103:107	arg1	source					139:144	the optimal nutrition source	117:144	the optimal nutrition source for infants	117:156	Human milk is the optimal nutrition source for infants, and oligosaccharides represent the third most abundant component in milk after lactose and fat.
33328537	1	18	theme	Human	103:107	arg1	milk					109:112	Human milk	103:112	Human milk	103:112	Human milk is the optimal nutrition source for infants, and oligosaccharides represent the third most abundant component in milk after lactose and fat.
33328537	1	19	theme	third	194:198	arg1	component					214:222	the third most abundant component	190:222	the third most abundant component in milk after lactose and fat	190:252	Human milk is the optimal nutrition source for infants, and oligosaccharides represent the third most abundant component in milk after lactose and fat.
33328537	5	20	theme	milk	945:948	arg1	bacteria					950:957	milk bacteria	945:957	milk bacteria	945:957	We find significant associations between the concentration of HMO-bound fucose and the abundance of fucosidase (a bacterial gene that digests fucose moieties) harbored by milk bacteria.
33328537	6	21	theme	infant	1138:1143	arg1	ingestion					1145:1153	infant ingestion	1138:1153	infant ingestion	1138:1153	These studies reveal a successive mechanism by which the maternal diet during lactation alters milk HMO composition, which in turn shapes the functional milk microbiome prior to infant ingestion.
33328537	6	22	theme	milk	1055:1058	arg1	composition					1064:1074	milk HMO composition	1055:1074	milk HMO composition	1055:1074	These studies reveal a successive mechanism by which the maternal diet during lactation alters milk HMO composition, which in turn shapes the functional milk microbiome prior to infant ingestion.
33328537	0	23	theme	oligosaccharide	32:46	arg1	composition					48:58	human milk oligosaccharide composition	21:58	human milk oligosaccharide composition	21:58	Maternal diet alters human milk oligosaccharide composition with implications for the milk metagenome.
33328537	1	24	theme	abundant	205:212	arg1	component					214:222	the third most abundant component	190:222	the third most abundant component in milk after lactose and fat	190:252	Human milk is the optimal nutrition source for infants, and oligosaccharides represent the third most abundant component in milk after lactose and fat.
33328537	0	25	theme	milk	27:30	arg1	composition					48:58	human milk oligosaccharide composition	21:58	human milk oligosaccharide composition	21:58	Maternal diet alters human milk oligosaccharide composition with implications for the milk metagenome.
33328537	5	26	theme	significant	782:792	arg1	associations					794:805	significant associations	782:805	significant associations between the concentration of HMO-bound fucose and the abundance of fucosidase (a bacterial gene that digests fucose moieties) harbored by milk bacteria	782:957	We find significant associations between the concentration of HMO-bound fucose and the abundance of fucosidase (a bacterial gene that digests fucose moieties) harbored by milk bacteria.
33328537	6	27	theme	maternal	1017:1024	arg1	diet					1026:1029	the maternal diet	1013:1029	the maternal diet during lactation	1013:1046	These studies reveal a successive mechanism by which the maternal diet during lactation alters milk HMO composition, which in turn shapes the functional milk microbiome prior to infant ingestion.
33328537	3	28	theme	milk	558:561	arg1	bacteria					563:570	the milk bacteria	554:570	the milk bacteria	554:570	Hypothesizing that the maternal diet itself might influence HMO composition, we sought to directly determine the effect maternal diet on HMO and the milk bacteria.
33328537	1	29	from	component	214:222	arg1	milk					227:230	milk	227:230	milk	227:230	Human milk is the optimal nutrition source for infants, and oligosaccharides represent the third most abundant component in milk after lactose and fat.
33328537	5	30	theme	fucose	916:921	arg1	moieties					923:930	fucose moieties	916:930	fucose moieties	916:930	We find significant associations between the concentration of HMO-bound fucose and the abundance of fucosidase (a bacterial gene that digests fucose moieties) harbored by milk bacteria.
33328537	5	31	dep	fucosidase	874:883	arg1	gene					898:901	a bacterial gene	886:901	a bacterial gene that digests fucose moieties	886:930	We find significant associations between the concentration of HMO-bound fucose and the abundance of fucosidase (a bacterial gene that digests fucose moieties) harbored by milk bacteria.
33328537	6	32	theme	prior	1129:1133	arg1	microbiome					1118:1127	the functional milk microbiome	1098:1127	the functional milk microbiome prior to infant ingestion	1098:1153	These studies reveal a successive mechanism by which the maternal diet during lactation alters milk HMO composition, which in turn shapes the functional milk microbiome prior to infant ingestion.
33328537	2	33	theme	Human	255:259	arg1	HMO					284:286	HMO	284:286	HMO	284:286	Human milk oligosaccharides (HMO) are favorable macromolecules which are, interestingly, indigestible by the infant but serve as substrates for bacteria.
33328537	2	33	theme	Human	255:259	arg1	oligosaccharides					266:281	Human milk oligosaccharides	255:281	Human milk oligosaccharides (HMO)	255:287	Human milk oligosaccharides (HMO) are favorable macromolecules which are, interestingly, indigestible by the infant but serve as substrates for bacteria.
33328537	2	33	theme	Human	255:259	arg1	macromolecules					303:316	favorable macromolecules	293:316	favorable macromolecules which are, interestingly, indigestible by the infant but serve as substrates for bacteria	293:406	Human milk oligosaccharides (HMO) are favorable macromolecules which are, interestingly, indigestible by the infant but serve as substrates for bacteria.
33328537	1	34	theme	optimal	121:127	arg1	source					139:144	the optimal nutrition source	117:144	the optimal nutrition source for infants	117:156	Human milk is the optimal nutrition source for infants, and oligosaccharides represent the third most abundant component in milk after lactose and fat.
33328537	1	34	theme	optimal	121:127	arg1	milk					109:112	Human milk	103:112	Human milk	103:112	Human milk is the optimal nutrition source for infants, and oligosaccharides represent the third most abundant component in milk after lactose and fat.
33328537	6	35	theme	milk	1113:1116	arg1	microbiome					1118:1127	the functional milk microbiome	1098:1127	the functional milk microbiome prior to infant ingestion	1098:1153	These studies reveal a successive mechanism by which the maternal diet during lactation alters milk HMO composition, which in turn shapes the functional milk microbiome prior to infant ingestion.
33328537	4	36	theme	human	585:589	arg1	design					608:613	a human cross-over study design	583:613	a human cross-over study design	583:613	Employing a human cross-over study design, we demonstrate that distinct maternal dietary carbohydrate and energy sources preferentially alter milk concentrations of HMO, including fucosylated species.
33328537	1	37	theme	nutrition	129:137	arg1	source					139:144	the optimal nutrition source	117:144	the optimal nutrition source for infants	117:156	Human milk is the optimal nutrition source for infants, and oligosaccharides represent the third most abundant component in milk after lactose and fat.
33328537	1	37	theme	nutrition	129:137	arg1	milk					109:112	Human milk	103:112	Human milk	103:112	Human milk is the optimal nutrition source for infants, and oligosaccharides represent the third most abundant component in milk after lactose and fat.
33328537	3	38	theme	HMO	469:471	arg1	composition					473:483	HMO composition	469:483	HMO composition	469:483	Hypothesizing that the maternal diet itself might influence HMO composition, we sought to directly determine the effect maternal diet on HMO and the milk bacteria.
33328537	4	39	gly	fucosylated	753:763	arg1	species					765:771	fucosylated species	753:771	fucosylated species	753:771	Employing a human cross-over study design, we demonstrate that distinct maternal dietary carbohydrate and energy sources preferentially alter milk concentrations of HMO, including fucosylated species.
33328537	5	40	theme	fucose	846:851	arg1	concentration					819:831	the concentration	815:831	the concentration of HMO-bound fucose	815:851	We find significant associations between the concentration of HMO-bound fucose and the abundance of fucosidase (a bacterial gene that digests fucose moieties) harbored by milk bacteria.
33328537	5	40	theme	fucose	846:851	arg1	abundance					861:869	the abundance	857:869	the abundance of fucosidase (a bacterial gene that digests fucose moieties) harbored by milk bacteria	857:957	We find significant associations between the concentration of HMO-bound fucose and the abundance of fucosidase (a bacterial gene that digests fucose moieties) harbored by milk bacteria.
33328537	5	41	theme	bacterial	888:896	arg1	gene					898:901	a bacterial gene	886:901	a bacterial gene that digests fucose moieties	886:930	We find significant associations between the concentration of HMO-bound fucose and the abundance of fucosidase (a bacterial gene that digests fucose moieties) harbored by milk bacteria.
33328537	5	42	theme	fucosidase	874:883	arg1	concentration					819:831	the concentration	815:831	the concentration of HMO-bound fucose	815:851	We find significant associations between the concentration of HMO-bound fucose and the abundance of fucosidase (a bacterial gene that digests fucose moieties) harbored by milk bacteria.
33328537	5	42	theme	fucosidase	874:883	arg1	abundance					861:869	the abundance	857:869	the abundance of fucosidase (a bacterial gene that digests fucose moieties) harbored by milk bacteria	857:957	We find significant associations between the concentration of HMO-bound fucose and the abundance of fucosidase (a bacterial gene that digests fucose moieties) harbored by milk bacteria.
33328537	4	43	theme	study	602:606	arg1	design					608:613	a human cross-over study design	583:613	a human cross-over study design	583:613	Employing a human cross-over study design, we demonstrate that distinct maternal dietary carbohydrate and energy sources preferentially alter milk concentrations of HMO, including fucosylated species.
33328537	6	44	theme	functional	1102:1111	arg1	microbiome					1118:1127	the functional milk microbiome	1098:1127	the functional milk microbiome prior to infant ingestion	1098:1153	These studies reveal a successive mechanism by which the maternal diet during lactation alters milk HMO composition, which in turn shapes the functional milk microbiome prior to infant ingestion.
33328537	5	45	theme	HMO-bound	836:844	arg1	fucose					846:851	HMO-bound fucose	836:851	HMO-bound fucose	836:851	We find significant associations between the concentration of HMO-bound fucose and the abundance of fucosidase (a bacterial gene that digests fucose moieties) harbored by milk bacteria.
33328537	4	46	theme	cross-over	591:600	arg1	design					608:613	a human cross-over study design	583:613	a human cross-over study design	583:613	Employing a human cross-over study design, we demonstrate that distinct maternal dietary carbohydrate and energy sources preferentially alter milk concentrations of HMO, including fucosylated species.
33321622	0	0	theme	guided	91:96	arg1	membranes					127:135	guided tissue regeneration hydrogel membranes	91:135	guided tissue regeneration hydrogel membranes with antibacterial and biocompatible properties	91:183	Chemical cross-linking on gelatin-hyaluronan loaded with hinokitiol for the preparation of guided tissue regeneration hydrogel membranes with antibacterial and biocompatible properties.
33321622	11	1	theme	functionalized	1746:1759	arg1	G/HA-Ec-2H					1770:1779	G/HA-Ec-2H	1770:1779	G/HA-Ec-2H	1770:1779	In summary, the membrane biostability of the cross-linked gelatin/hyaluronan hydrogels was enhanced by EDC at a biocompatible concentration, and the functionalized group of G/HA-Ec-2H shows potential as a biodegradable material for biocompatible tissue-guarded regeneration membranes with antibacterial properties.
33321622	11	1	theme	functionalized	1746:1759	arg1	group					1761:1765	the functionalized group	1742:1765	the functionalized group of G/HA-Ec-2H	1742:1779	In summary, the membrane biostability of the cross-linked gelatin/hyaluronan hydrogels was enhanced by EDC at a biocompatible concentration, and the functionalized group of G/HA-Ec-2H shows potential as a biodegradable material for biocompatible tissue-guarded regeneration membranes with antibacterial properties.
33321622	7	2	theme	sample	1195:1200	arg1	morphology					1202:1211	sample morphology	1195:1211	sample morphology	1195:1211	The rate of hydrogel degradation decreased, and the integrity of sample morphology was maintained at more than 80% for over 3 days in the immersion.
33321622	9	3	theme	biocompatibility	1389:1404	arg1	results					1406:1412	The biocompatibility results	1385:1412	The biocompatibility results	1385:1412	The biocompatibility results showed that low concentrations of hinokitiol did not affect cell viability.
33321622	8	4	theme	degradation	1351:1361	arg1	reaction					1363:1370	a rapid degradation reaction	1343:1370	a rapid degradation reaction within 24 h	1343:1382	Then, the hydrogel structures relaxed and disintegrated through a rapid degradation reaction within 24 h.
33321622	0	5	from	cross-linking	9:21	arg1	gelatin-hyaluronan					26:43	gelatin-hyaluronan	26:43	gelatin-hyaluronan	26:43	Chemical cross-linking on gelatin-hyaluronan loaded with hinokitiol for the preparation of guided tissue regeneration hydrogel membranes with antibacterial and biocompatible properties.
33321622	4	6	dep	hinokitiol	851:860	arg1	G/HA-Ec-4H					878:887	G/HA-Ec-4H	878:887	G/HA-Ec-4H	878:887	The membranes were cross-linked with EDC (G/HA-Ec-0H) and impregnated with two concentrations of the antibacterial agent of hinokitiol (G/HA-Ec-2H and G/HA-Ec-4H).
33321622	4	6	dep	hinokitiol	851:860	arg1	G/HA-Ec-2H					863:872	G/HA-Ec-2H	863:872	G/HA-Ec-2H	863:872	The membranes were cross-linked with EDC (G/HA-Ec-0H) and impregnated with two concentrations of the antibacterial agent of hinokitiol (G/HA-Ec-2H and G/HA-Ec-4H).
33321622	1	7	theme	mechanical	190:199	arg1	properties					201:210	mechanical properties	190:210	mechanical properties	190:210	The mechanical properties and structural stability of hydrogels and their performance in antidegradation can be enhanced by cross-linking them with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC).
33321622	4	8	theme	antibacterial	828:840	arg1	agent					842:846	the antibacterial agent	824:846	the antibacterial agent of hinokitiol (G/HA-Ec-2H and G/HA-Ec-4H)	824:888	The membranes were cross-linked with EDC (G/HA-Ec-0H) and impregnated with two concentrations of the antibacterial agent of hinokitiol (G/HA-Ec-2H and G/HA-Ec-4H).
33321622	0	9	theme	regeneration	105:116	arg1	membranes					127:135	guided tissue regeneration hydrogel membranes	91:135	guided tissue regeneration hydrogel membranes with antibacterial and biocompatible properties	91:183	Chemical cross-linking on gelatin-hyaluronan loaded with hinokitiol for the preparation of guided tissue regeneration hydrogel membranes with antibacterial and biocompatible properties.
33321622	3	10	theme	antibacterial	620:632	arg1	effects					634:640	antibacterial effects	620:640	antibacterial effects	620:640	In this study, a facile process for preparing hydrogel regenerative membranes exerting antibacterial effects and containing gelatin/hyaluronic acid (G/HA) through solution casting was proposed.
33321622	0	11	theme	tissue	98:103	arg1	regeneration					105:116	tissue regeneration	98:116	guided tissue regeneration hydrogel membranes with antibacterial and biocompatible properties	91:183	Chemical cross-linking on gelatin-hyaluronan loaded with hinokitiol for the preparation of guided tissue regeneration hydrogel membranes with antibacterial and biocompatible properties.
33321622	11	12	theme	G/HA-Ec-2H	1770:1779	arg1	G/HA-Ec-2H					1770:1779	G/HA-Ec-2H	1770:1779	G/HA-Ec-2H	1770:1779	In summary, the membrane biostability of the cross-linked gelatin/hyaluronan hydrogels was enhanced by EDC at a biocompatible concentration, and the functionalized group of G/HA-Ec-2H shows potential as a biodegradable material for biocompatible tissue-guarded regeneration membranes with antibacterial properties.
33321622	11	12	theme	G/HA-Ec-2H	1770:1779	arg1	group					1761:1765	the functionalized group	1742:1765	the functionalized group of G/HA-Ec-2H	1742:1779	In summary, the membrane biostability of the cross-linked gelatin/hyaluronan hydrogels was enhanced by EDC at a biocompatible concentration, and the functionalized group of G/HA-Ec-2H shows potential as a biodegradable material for biocompatible tissue-guarded regeneration membranes with antibacterial properties.
33321622	5	13	theme	cross-linking	1024:1036	arg1	degree					1014:1019	the degree	1010:1019	the degree of cross-linking	1010:1036	Amide bonds formed, and the rate of active amino acid fixation was higher than 90%, which was directly proportional to the degree of cross-linking.
33321622	4	14	theme	hinokitiol	851:860	arg1	agent					842:846	the antibacterial agent	824:846	the antibacterial agent of hinokitiol (G/HA-Ec-2H and G/HA-Ec-4H)	824:888	The membranes were cross-linked with EDC (G/HA-Ec-0H) and impregnated with two concentrations of the antibacterial agent of hinokitiol (G/HA-Ec-2H and G/HA-Ec-4H).
33321622	11	15	theme	gelatin/hyaluronan	1655:1672	arg1	hydrogels					1674:1682	the cross-linked gelatin/hyaluronan hydrogels	1638:1682	the cross-linked gelatin/hyaluronan hydrogels	1638:1682	In summary, the membrane biostability of the cross-linked gelatin/hyaluronan hydrogels was enhanced by EDC at a biocompatible concentration, and the functionalized group of G/HA-Ec-2H shows potential as a biodegradable material for biocompatible tissue-guarded regeneration membranes with antibacterial properties.
33321622	6	16	theme	G/HA-Ec-2H	1043:1052	arg1	groups					1069:1074	The G/HA-Ec-2H and G/HA-Ec-4H groups	1039:1074	The G/HA-Ec-2H and G/HA-Ec-4H groups with hinokitiol	1039:1090	The G/HA-Ec-2H and G/HA-Ec-4H groups with hinokitiol showed good antibacterial properties.
33321622	0	17	theme	membranes	127:135	arg1	preparation					76:86	the preparation	72:86	the preparation of guided tissue regeneration hydrogel membranes with antibacterial and biocompatible properties	72:183	Chemical cross-linking on gelatin-hyaluronan loaded with hinokitiol for the preparation of guided tissue regeneration hydrogel membranes with antibacterial and biocompatible properties.
33321622	3	18	dep	membranes	601:609	arg1	containing					646:655	containing	646:655	containing gelatin/hyaluronic acid (G/HA)	646:686	In this study, a facile process for preparing hydrogel regenerative membranes exerting antibacterial effects and containing gelatin/hyaluronic acid (G/HA) through solution casting was proposed.
33321622	3	18	dep	membranes	601:609	arg1	exerting					611:618	exerting	611:618	exerting antibacterial effects	611:640	In this study, a facile process for preparing hydrogel regenerative membranes exerting antibacterial effects and containing gelatin/hyaluronic acid (G/HA) through solution casting was proposed.
33321622	10	19	theme	good	1559:1562	arg1	adhesion					1569:1576	good cell adhesion	1559:1576	good cell adhesion	1559:1576	Moreover, hydrogel membranes after 14 days of cell incubation showed good cell adhesion and proliferation.
33321622	2	20	link	un-cross-linked	506:520	arg1	hydrogels					522:530	un-cross-linked hydrogels	506:530	un-cross-linked hydrogels	506:530	However, residual EDC compromises the biocompatibility of cross-linked hydrogels and the formability of un-cross-linked hydrogels.
33321622	5	21	theme	fixation	945:952	arg1	higher					958:963	higher	958:963	higher	958:963	Amide bonds formed, and the rate of active amino acid fixation was higher than 90%, which was directly proportional to the degree of cross-linking.
33321622	5	21	theme	fixation	945:952	arg1	rate					919:922	the rate	915:922	the rate of active amino acid fixation	915:952	Amide bonds formed, and the rate of active amino acid fixation was higher than 90%, which was directly proportional to the degree of cross-linking.
33321622	5	22	theme	amino	934:938	arg1	fixation					945:952	active amino acid fixation	927:952	active amino acid fixation	927:952	Amide bonds formed, and the rate of active amino acid fixation was higher than 90%, which was directly proportional to the degree of cross-linking.
33321622	0	23	theme	hydrogel	118:125	arg1	membranes					127:135	guided tissue regeneration hydrogel membranes	91:135	guided tissue regeneration hydrogel membranes with antibacterial and biocompatible properties	91:183	Chemical cross-linking on gelatin-hyaluronan loaded with hinokitiol for the preparation of guided tissue regeneration hydrogel membranes with antibacterial and biocompatible properties.
33321622	0	24	with	membranes	127:135	arg1	properties					174:183	antibacterial and biocompatible properties	142:183	antibacterial and biocompatible properties	142:183	Chemical cross-linking on gelatin-hyaluronan loaded with hinokitiol for the preparation of guided tissue regeneration hydrogel membranes with antibacterial and biocompatible properties.
33321622	2	25	theme	un-cross-linked	506:520	arg1	hydrogels					522:530	un-cross-linked hydrogels	506:530	un-cross-linked hydrogels	506:530	However, residual EDC compromises the biocompatibility of cross-linked hydrogels and the formability of un-cross-linked hydrogels.
33321622	5	26	theme	acid	940:943	arg1	fixation					945:952	active amino acid fixation	927:952	active amino acid fixation	927:952	Amide bonds formed, and the rate of active amino acid fixation was higher than 90%, which was directly proportional to the degree of cross-linking.
33321622	2	27	theme	hydrogels	522:530	arg1	biocompatibility					440:455	the biocompatibility	436:455	the biocompatibility of cross-linked hydrogels	436:481	However, residual EDC compromises the biocompatibility of cross-linked hydrogels and the formability of un-cross-linked hydrogels.
33321622	2	27	theme	hydrogels	522:530	arg1	formability					491:501	the formability	487:501	the formability of un-cross-linked hydrogels	487:530	However, residual EDC compromises the biocompatibility of cross-linked hydrogels and the formability of un-cross-linked hydrogels.
33321622	6	28	theme	antibacterial	1104:1116	arg1	properties					1118:1127	good antibacterial properties	1099:1127	good antibacterial properties	1099:1127	The G/HA-Ec-2H and G/HA-Ec-4H groups with hinokitiol showed good antibacterial properties.
33321622	10	29	theme	incubation	1541:1550	arg1	14 days					1525:1531	14 days	1525:1531	14 days of cell incubation	1525:1550	Moreover, hydrogel membranes after 14 days of cell incubation showed good cell adhesion and proliferation.
33321622	11	30	theme	hydrogels	1674:1682	arg1	biostability					1622:1633	the membrane biostability	1609:1633	the membrane biostability of the cross-linked gelatin/hyaluronan hydrogels	1609:1682	In summary, the membrane biostability of the cross-linked gelatin/hyaluronan hydrogels was enhanced by EDC at a biocompatible concentration, and the functionalized group of G/HA-Ec-2H shows potential as a biodegradable material for biocompatible tissue-guarded regeneration membranes with antibacterial properties.
33321622	3	31	theme	gelatin/hyaluronic	657:674	arg1	G/HA					682:685	G/HA	682:685	G/HA	682:685	In this study, a facile process for preparing hydrogel regenerative membranes exerting antibacterial effects and containing gelatin/hyaluronic acid (G/HA) through solution casting was proposed.
33321622	3	31	theme	gelatin/hyaluronic	657:674	arg1	acid					676:679	gelatin/hyaluronic acid	657:679	gelatin/hyaluronic acid (G/HA)	657:686	In this study, a facile process for preparing hydrogel regenerative membranes exerting antibacterial effects and containing gelatin/hyaluronic acid (G/HA) through solution casting was proposed.
33321622	1	32	theme	structural	216:225	arg1	stability					227:235	structural stability	216:235	structural stability	216:235	The mechanical properties and structural stability of hydrogels and their performance in antidegradation can be enhanced by cross-linking them with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC).
33321622	0	33	theme	Chemical	0:7	arg1	cross-linking					9:21	Chemical cross-linking	0:21	Chemical cross-linking on gelatin-hyaluronan	0:43	Chemical cross-linking on gelatin-hyaluronan loaded with hinokitiol for the preparation of guided tissue regeneration hydrogel membranes with antibacterial and biocompatible properties.
33321622	8	34	theme	hydrogel	1289:1296	arg1	structures					1298:1307	the hydrogel structures	1285:1307	the hydrogel structures	1285:1307	Then, the hydrogel structures relaxed and disintegrated through a rapid degradation reaction within 24 h.
33321622	9	35	theme	hinokitiol	1448:1457	arg1	concentrations					1430:1443	low concentrations	1426:1443	low concentrations of hinokitiol	1426:1457	The biocompatibility results showed that low concentrations of hinokitiol did not affect cell viability.
33321622	7	36	theme	morphology	1202:1211	arg1	integrity					1182:1190	the integrity	1178:1190	the integrity of sample morphology	1178:1211	The rate of hydrogel degradation decreased, and the integrity of sample morphology was maintained at more than 80% for over 3 days in the immersion.
33321622	6	37	with	groups	1069:1074	arg1	hinokitiol					1081:1090	hinokitiol	1081:1090	hinokitiol	1081:1090	The G/HA-Ec-2H and G/HA-Ec-4H groups with hinokitiol showed good antibacterial properties.
33321622	0	38	theme	antibacterial	142:154	arg1	properties					174:183	antibacterial and biocompatible properties	142:183	antibacterial and biocompatible properties	142:183	Chemical cross-linking on gelatin-hyaluronan loaded with hinokitiol for the preparation of guided tissue regeneration hydrogel membranes with antibacterial and biocompatible properties.
33321622	11	39	theme	membrane	1613:1620	arg1	biostability					1622:1633	the membrane biostability	1609:1633	the membrane biostability of the cross-linked gelatin/hyaluronan hydrogels	1609:1682	In summary, the membrane biostability of the cross-linked gelatin/hyaluronan hydrogels was enhanced by EDC at a biocompatible concentration, and the functionalized group of G/HA-Ec-2H shows potential as a biodegradable material for biocompatible tissue-guarded regeneration membranes with antibacterial properties.
33321622	10	40	theme	cell	1536:1539	arg1	incubation					1541:1550	cell incubation	1536:1550	cell incubation	1536:1550	Moreover, hydrogel membranes after 14 days of cell incubation showed good cell adhesion and proliferation.
33321622	11	41	theme	antibacterial	1886:1898	arg1	properties					1900:1909	antibacterial properties	1886:1909	antibacterial properties	1886:1909	In summary, the membrane biostability of the cross-linked gelatin/hyaluronan hydrogels was enhanced by EDC at a biocompatible concentration, and the functionalized group of G/HA-Ec-2H shows potential as a biodegradable material for biocompatible tissue-guarded regeneration membranes with antibacterial properties.
33321622	7	42	theme	degradation	1151:1161	arg1	rate					1134:1137	The rate	1130:1137	The rate of hydrogel degradation	1130:1161	The rate of hydrogel degradation decreased, and the integrity of sample morphology was maintained at more than 80% for over 3 days in the immersion.
33321622	4	43	theme	agent	842:846	arg1	concentrations					806:819	two concentrations	802:819	two concentrations of the antibacterial agent of hinokitiol (G/HA-Ec-2H and G/HA-Ec-4H)	802:888	The membranes were cross-linked with EDC (G/HA-Ec-0H) and impregnated with two concentrations of the antibacterial agent of hinokitiol (G/HA-Ec-2H and G/HA-Ec-4H).
33321622	1	44	theme	hydrogels	240:248	arg1	properties					201:210	mechanical properties	190:210	mechanical properties	190:210	The mechanical properties and structural stability of hydrogels and their performance in antidegradation can be enhanced by cross-linking them with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC).
33321622	1	44	theme	hydrogels	240:248	arg1	stability					227:235	structural stability	216:235	structural stability	216:235	The mechanical properties and structural stability of hydrogels and their performance in antidegradation can be enhanced by cross-linking them with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC).
33321622	9	45	theme	low	1426:1428	arg1	concentrations					1430:1443	low concentrations	1426:1443	low concentrations of hinokitiol	1426:1457	The biocompatibility results showed that low concentrations of hinokitiol did not affect cell viability.
33321622	0	46	theme	biocompatible	160:172	arg1	properties					174:183	antibacterial and biocompatible properties	142:183	antibacterial and biocompatible properties	142:183	Chemical cross-linking on gelatin-hyaluronan loaded with hinokitiol for the preparation of guided tissue regeneration hydrogel membranes with antibacterial and biocompatible properties.
33321622	7	47	from	3 days	1254:1259	arg1	immersion					1268:1276	the immersion	1264:1276	the immersion	1264:1276	The rate of hydrogel degradation decreased, and the integrity of sample morphology was maintained at more than 80% for over 3 days in the immersion.
33321622	11	48	theme	biodegradable	1802:1814	arg1	potential					1787:1795	potential	1787:1795	potential	1787:1795	In summary, the membrane biostability of the cross-linked gelatin/hyaluronan hydrogels was enhanced by EDC at a biocompatible concentration, and the functionalized group of G/HA-Ec-2H shows potential as a biodegradable material for biocompatible tissue-guarded regeneration membranes with antibacterial properties.
33321622	11	48	theme	biodegradable	1802:1814	arg1	material					1816:1823	a biodegradable material	1800:1823	a biodegradable material for biocompatible tissue-guarded regeneration membranes with antibacterial properties	1800:1909	In summary, the membrane biostability of the cross-linked gelatin/hyaluronan hydrogels was enhanced by EDC at a biocompatible concentration, and the functionalized group of G/HA-Ec-2H shows potential as a biodegradable material for biocompatible tissue-guarded regeneration membranes with antibacterial properties.
33321622	2	49	theme	hydrogels	473:481	arg1	biocompatibility					440:455	the biocompatibility	436:455	the biocompatibility of cross-linked hydrogels	436:481	However, residual EDC compromises the biocompatibility of cross-linked hydrogels and the formability of un-cross-linked hydrogels.
33321622	2	49	theme	hydrogels	473:481	arg1	formability					491:501	the formability	487:501	the formability of un-cross-linked hydrogels	487:530	However, residual EDC compromises the biocompatibility of cross-linked hydrogels and the formability of un-cross-linked hydrogels.
33321622	6	50	theme	good	1099:1102	arg1	properties					1118:1127	good antibacterial properties	1099:1127	good antibacterial properties	1099:1127	The G/HA-Ec-2H and G/HA-Ec-4H groups with hinokitiol showed good antibacterial properties.
33321622	6	51	theme	G/HA-Ec-4H	1058:1067	arg1	groups					1069:1074	The G/HA-Ec-2H and G/HA-Ec-4H groups	1039:1074	The G/HA-Ec-2H and G/HA-Ec-4H groups with hinokitiol	1039:1090	The G/HA-Ec-2H and G/HA-Ec-4H groups with hinokitiol showed good antibacterial properties.
33321622	10	52	theme	cell	1564:1567	arg1	adhesion					1569:1576	good cell adhesion	1559:1576	good cell adhesion	1559:1576	Moreover, hydrogel membranes after 14 days of cell incubation showed good cell adhesion and proliferation.
33321622	2	53	theme	cross-linked	460:471	arg1	hydrogels					473:481	cross-linked hydrogels	460:481	cross-linked hydrogels	460:481	However, residual EDC compromises the biocompatibility of cross-linked hydrogels and the formability of un-cross-linked hydrogels.
33321622	1	54	from	antidegradation	275:289	arg1	properties					201:210	mechanical properties	190:210	mechanical properties	190:210	The mechanical properties and structural stability of hydrogels and their performance in antidegradation can be enhanced by cross-linking them with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC).
33321622	1	54	from	antidegradation	275:289	arg1	stability					227:235	structural stability	216:235	structural stability	216:235	The mechanical properties and structural stability of hydrogels and their performance in antidegradation can be enhanced by cross-linking them with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC).
33321622	1	55	dep	properties	201:210	arg1	The					186:188	The	186:188	The	186:188	The mechanical properties and structural stability of hydrogels and their performance in antidegradation can be enhanced by cross-linking them with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC).
33321622	11	56	theme	regeneration	1858:1869	arg1	membranes					1871:1879	biocompatible tissue-guarded regeneration membranes	1829:1879	biocompatible tissue-guarded regeneration membranes with antibacterial properties	1829:1909	In summary, the membrane biostability of the cross-linked gelatin/hyaluronan hydrogels was enhanced by EDC at a biocompatible concentration, and the functionalized group of G/HA-Ec-2H shows potential as a biodegradable material for biocompatible tissue-guarded regeneration membranes with antibacterial properties.
33321622	3	57	theme	facile	550:555	arg1	process					557:563	a facile process	548:563	a facile process for preparing hydrogel regenerative membranes exerting antibacterial effects and containing gelatin/hyaluronic acid (G/HA) through solution casting	548:711	In this study, a facile process for preparing hydrogel regenerative membranes exerting antibacterial effects and containing gelatin/hyaluronic acid (G/HA) through solution casting was proposed.
33321622	5	58	theme	Amide	891:895	arg1	bonds					897:901	Amide bonds	891:901	Amide bonds	891:901	Amide bonds formed, and the rate of active amino acid fixation was higher than 90%, which was directly proportional to the degree of cross-linking.
33321622	1	59	theme	-N'-ethylcarbodiimide	359:379	arg1	EDC					396:398	EDC	396:398	EDC	396:398	The mechanical properties and structural stability of hydrogels and their performance in antidegradation can be enhanced by cross-linking them with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC).
33321622	1	59	theme	-N'-ethylcarbodiimide	359:379	arg1	hydrochloride					381:393	N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride	334:393	N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC)	334:399	The mechanical properties and structural stability of hydrogels and their performance in antidegradation can be enhanced by cross-linking them with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC).
33321622	7	60	theme	hydrogel	1142:1149	arg1	degradation					1151:1161	hydrogel degradation	1142:1161	hydrogel degradation	1142:1161	The rate of hydrogel degradation decreased, and the integrity of sample morphology was maintained at more than 80% for over 3 days in the immersion.
33321622	11	61	theme	biocompatible	1829:1841	arg1	membranes					1871:1879	biocompatible tissue-guarded regeneration membranes	1829:1879	biocompatible tissue-guarded regeneration membranes with antibacterial properties	1829:1909	In summary, the membrane biostability of the cross-linked gelatin/hyaluronan hydrogels was enhanced by EDC at a biocompatible concentration, and the functionalized group of G/HA-Ec-2H shows potential as a biodegradable material for biocompatible tissue-guarded regeneration membranes with antibacterial properties.
33321622	11	62	theme	tissue-guarded	1843:1856	arg1	membranes					1871:1879	biocompatible tissue-guarded regeneration membranes	1829:1879	biocompatible tissue-guarded regeneration membranes with antibacterial properties	1829:1909	In summary, the membrane biostability of the cross-linked gelatin/hyaluronan hydrogels was enhanced by EDC at a biocompatible concentration, and the functionalized group of G/HA-Ec-2H shows potential as a biodegradable material for biocompatible tissue-guarded regeneration membranes with antibacterial properties.
33321622	11	63	with	membranes	1871:1879	arg1	properties					1900:1909	antibacterial properties	1886:1909	antibacterial properties	1886:1909	In summary, the membrane biostability of the cross-linked gelatin/hyaluronan hydrogels was enhanced by EDC at a biocompatible concentration, and the functionalized group of G/HA-Ec-2H shows potential as a biodegradable material for biocompatible tissue-guarded regeneration membranes with antibacterial properties.
33321622	8	64	theme	rapid	1345:1349	arg1	reaction					1363:1370	a rapid degradation reaction	1343:1370	a rapid degradation reaction within 24 h	1343:1382	Then, the hydrogel structures relaxed and disintegrated through a rapid degradation reaction within 24 h.
33321622	11	65	theme	cross-linked	1642:1653	arg1	hydrogels					1674:1682	the cross-linked gelatin/hyaluronan hydrogels	1638:1682	the cross-linked gelatin/hyaluronan hydrogels	1638:1682	In summary, the membrane biostability of the cross-linked gelatin/hyaluronan hydrogels was enhanced by EDC at a biocompatible concentration, and the functionalized group of G/HA-Ec-2H shows potential as a biodegradable material for biocompatible tissue-guarded regeneration membranes with antibacterial properties.
33321622	1	66	from	performance	260:270	arg1	antidegradation					275:289	antidegradation	275:289	antidegradation	275:289	The mechanical properties and structural stability of hydrogels and their performance in antidegradation can be enhanced by cross-linking them with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC).
33321622	2	67	link	cross-linked	460:471	arg1	hydrogels					473:481	cross-linked hydrogels	460:481	cross-linked hydrogels	460:481	However, residual EDC compromises the biocompatibility of cross-linked hydrogels and the formability of un-cross-linked hydrogels.
33321622	10	68	theme	hydrogel	1500:1507	arg1	membranes					1509:1517	hydrogel membranes	1500:1517	hydrogel membranes after 14 days of cell incubation	1500:1550	Moreover, hydrogel membranes after 14 days of cell incubation showed good cell adhesion and proliferation.
33321622	1	69	from	hydrogels	240:248	arg1	antidegradation					275:289	antidegradation	275:289	antidegradation	275:289	The mechanical properties and structural stability of hydrogels and their performance in antidegradation can be enhanced by cross-linking them with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC).
33321622	1	70	theme	performance	260:270	arg1	properties					201:210	mechanical properties	190:210	mechanical properties	190:210	The mechanical properties and structural stability of hydrogels and their performance in antidegradation can be enhanced by cross-linking them with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC).
33321622	1	70	theme	performance	260:270	arg1	stability					227:235	structural stability	216:235	structural stability	216:235	The mechanical properties and structural stability of hydrogels and their performance in antidegradation can be enhanced by cross-linking them with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC).
33321622	11	71	theme	biocompatible	1709:1721	arg1	concentration					1723:1735	a biocompatible concentration	1707:1735	a biocompatible concentration	1707:1735	In summary, the membrane biostability of the cross-linked gelatin/hyaluronan hydrogels was enhanced by EDC at a biocompatible concentration, and the functionalized group of G/HA-Ec-2H shows potential as a biodegradable material for biocompatible tissue-guarded regeneration membranes with antibacterial properties.
33321622	3	72	theme	regenerative	588:599	arg1	membranes					601:609	hydrogel regenerative membranes	579:609	hydrogel regenerative membranes exerting antibacterial effects and containing gelatin/hyaluronic acid (G/HA)	579:686	In this study, a facile process for preparing hydrogel regenerative membranes exerting antibacterial effects and containing gelatin/hyaluronic acid (G/HA) through solution casting was proposed.
33321622	11	73	link	cross-linked	1642:1653	arg1	hydrogels					1674:1682	the cross-linked gelatin/hyaluronan hydrogels	1638:1682	the cross-linked gelatin/hyaluronan hydrogels	1638:1682	In summary, the membrane biostability of the cross-linked gelatin/hyaluronan hydrogels was enhanced by EDC at a biocompatible concentration, and the functionalized group of G/HA-Ec-2H shows potential as a biodegradable material for biocompatible tissue-guarded regeneration membranes with antibacterial properties.
33321622	5	74	theme	active	927:932	arg1	fixation					945:952	active amino acid fixation	927:952	active amino acid fixation	927:952	Amide bonds formed, and the rate of active amino acid fixation was higher than 90%, which was directly proportional to the degree of cross-linking.
33321622	3	75	theme	solution	696:703	arg1	casting					705:711	solution casting	696:711	solution casting	696:711	In this study, a facile process for preparing hydrogel regenerative membranes exerting antibacterial effects and containing gelatin/hyaluronic acid (G/HA) through solution casting was proposed.
33321622	1	76	from	stability	227:235	arg1	antidegradation					275:289	antidegradation	275:289	antidegradation	275:289	The mechanical properties and structural stability of hydrogels and their performance in antidegradation can be enhanced by cross-linking them with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC).
33321622	3	77	theme	hydrogel	579:586	arg1	membranes					601:609	hydrogel regenerative membranes	579:609	hydrogel regenerative membranes exerting antibacterial effects and containing gelatin/hyaluronic acid (G/HA)	579:686	In this study, a facile process for preparing hydrogel regenerative membranes exerting antibacterial effects and containing gelatin/hyaluronic acid (G/HA) through solution casting was proposed.
33321622	9	78	theme	cell	1474:1477	arg1	viability					1479:1487	cell viability	1474:1487	cell viability	1474:1487	The biocompatibility results showed that low concentrations of hinokitiol did not affect cell viability.
33321622	1	79	from	properties	201:210	arg1	antidegradation					275:289	antidegradation	275:289	antidegradation	275:289	The mechanical properties and structural stability of hydrogels and their performance in antidegradation can be enhanced by cross-linking them with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC).
33321622	2	80	theme	residual	411:418	arg1	EDC					420:422	residual EDC	411:422	residual EDC	411:422	However, residual EDC compromises the biocompatibility of cross-linked hydrogels and the formability of un-cross-linked hydrogels.
32750473	0	0	theme	Cissus	78:83	arg1	plant					95:99	Cissus vitiginea plant	78:99	Cissus vitiginea plant	78:99	Characterization of novel natural cellulosic fiber extracted from the stem of Cissus vitiginea plant.
32750473	2	1	theme	mechanical	411:420	arg1	characteristics					422:436	better mechanical characteristics	404:436	better mechanical characteristics	404:436	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	3	2	theme	Various	439:445	arg1	groups					456:461	Various chemical groups	439:461	Various chemical groups distributed over the fiber surface	439:496	Various chemical groups distributed over the fiber surface were predicted and reported with the help of Fourier transform infrared spectroscope and nuclear magnetic response spectroscopy.
32750473	2	3	theme	better	404:409	arg1	characteristics					422:436	better mechanical characteristics	404:436	better mechanical characteristics	404:436	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	3	4	theme	chemical	447:454	arg1	groups					456:461	Various chemical groups	439:461	Various chemical groups distributed over the fiber surface	439:496	Various chemical groups distributed over the fiber surface were predicted and reported with the help of Fourier transform infrared spectroscope and nuclear magnetic response spectroscopy.
32750473	0	5	theme	plant	95:99	arg1	stem					70:73	the stem	66:73	the stem of Cissus vitiginea plant	66:99	Characterization of novel natural cellulosic fiber extracted from the stem of Cissus vitiginea plant.
32750473	7	6	theme	thermoplastic	1069:1081	arg1	composite					1089:1097	the thermoplastic green composite	1065:1097	the thermoplastic green composite	1065:1097	Hence C. vitiginea fiber can be suggested as reinforcement for the thermoplastic green composite.
32750473	3	7	theme	fiber	484:488	arg1	surface					490:496	the fiber surface	480:496	the fiber surface	480:496	Various chemical groups distributed over the fiber surface were predicted and reported with the help of Fourier transform infrared spectroscope and nuclear magnetic response spectroscopy.
32750473	4	8	theme	diffraction	696:706	arg1	spectroscopy					708:719	X-ray diffraction spectroscopy	690:719	X-ray diffraction spectroscopy	690:719	The crystalline nature of the fiber surface was examined using X-ray diffraction spectroscopy and the crystallinity index value was calculated to be 30.5% with 12.69 nm crystallite size.
32750473	7	9	theme	green	1083:1087	arg1	composite					1089:1097	the thermoplastic green composite	1065:1097	the thermoplastic green composite	1065:1097	Hence C. vitiginea fiber can be suggested as reinforcement for the thermoplastic green composite.
32750473	6	10	theme	thermal	903:909	arg1	stability					911:919	The thermal stability	899:919	The thermal stability of the fiber	899:932	The thermal stability of the fiber was found to be 304 °C with 68.72 kJ/mol kinetic activation energy.
32750473	4	11	theme	fiber	657:661	arg1	surface					663:669	the fiber surface	653:669	the fiber surface	653:669	The crystalline nature of the fiber surface was examined using X-ray diffraction spectroscopy and the crystallinity index value was calculated to be 30.5% with 12.69 nm crystallite size.
32750473	5	12	theme	scanning	869:876	arg1	microscope					887:896	a scanning electron microscope	867:896	a scanning electron microscope	867:896	Morphological study was conducted on the fiber using a scanning electron microscope.
32750473	3	13	dep	transform	551:559	arg1	infrared					561:568	infrared	561:568	transform infrared spectroscope and nuclear magnetic response spectroscopy	551:624	Various chemical groups distributed over the fiber surface were predicted and reported with the help of Fourier transform infrared spectroscope and nuclear magnetic response spectroscopy.
32750473	4	14	theme	crystalline	631:641	arg1	nature					643:648	The crystalline nature	627:648	The crystalline nature of the fiber surface	627:669	The crystalline nature of the fiber surface was examined using X-ray diffraction spectroscopy and the crystallinity index value was calculated to be 30.5% with 12.69 nm crystallite size.
32750473	4	15	theme	crystallite	796:806	arg1	size					808:811	12.69 nm crystallite size	787:811	12.69 nm crystallite size	787:811	The crystalline nature of the fiber surface was examined using X-ray diffraction spectroscopy and the crystallinity index value was calculated to be 30.5% with 12.69 nm crystallite size.
32750473	4	16	theme	index	743:747	arg1	%					780:780	30.5%	776:780	30.5% with 12.69 nm crystallite size	776:811	The crystalline nature of the fiber surface was examined using X-ray diffraction spectroscopy and the crystallinity index value was calculated to be 30.5% with 12.69 nm crystallite size.
32750473	4	16	theme	index	743:747	arg1	value					749:753	the crystallinity index value	725:753	the crystallinity index value	725:753	The crystalline nature of the fiber surface was examined using X-ray diffraction spectroscopy and the crystallinity index value was calculated to be 30.5% with 12.69 nm crystallite size.
32750473	2	17	theme	quantity	333:340	arg1	quantity					333:340	lower quantity	327:340	lower quantity of hemicellulose compounds (14.61 wt%)	327:379	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	17	theme	quantity	333:340	arg1	%					378:378	14.61 wt%	370:378	14.61 wt%	370:378	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	17	theme	quantity	333:340	arg1	cellulose					301:309	cellulose	301:309	cellulose (65.43 wt%)	301:321	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	17	theme	quantity	333:340	arg1	%					320:320	65.43 wt%	312:320	65.43 wt%	312:320	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	17	theme	quantity	333:340	arg1	amount					291:296	a rich amount	284:296	a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics	284:436	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	0	18	dep	Cissus	78:83	arg1	vitiginea					85:93	vitiginea	85:93	vitiginea	85:93	Characterization of novel natural cellulosic fiber extracted from the stem of Cissus vitiginea plant.
32750473	2	19	theme	lower	327:331	arg1	quantity					333:340	lower quantity	327:340	lower quantity of hemicellulose compounds (14.61 wt%)	327:379	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	19	theme	lower	327:331	arg1	%					378:378	14.61 wt%	370:378	14.61 wt%	370:378	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	4	20	theme	X-ray	690:694	arg1	spectroscopy					708:719	X-ray diffraction spectroscopy	690:719	X-ray diffraction spectroscopy	690:719	The crystalline nature of the fiber surface was examined using X-ray diffraction spectroscopy and the crystallinity index value was calculated to be 30.5% with 12.69 nm crystallite size.
32750473	4	21	theme	crystallinity	729:741	arg1	%					780:780	30.5%	776:780	30.5% with 12.69 nm crystallite size	776:811	The crystalline nature of the fiber surface was examined using X-ray diffraction spectroscopy and the crystallinity index value was calculated to be 30.5% with 12.69 nm crystallite size.
32750473	4	21	theme	crystallinity	729:741	arg1	value					749:753	the crystallinity index value	725:753	the crystallinity index value	725:753	The crystalline nature of the fiber surface was examined using X-ray diffraction spectroscopy and the crystallinity index value was calculated to be 30.5% with 12.69 nm crystallite size.
32750473	0	22	theme	natural	26:32	arg1	fiber					45:49	novel natural cellulosic fiber	20:49	novel natural cellulosic fiber	20:49	Characterization of novel natural cellulosic fiber extracted from the stem of Cissus vitiginea plant.
32750473	1	23	theme	Cissus	163:168	arg1	vitiginea					170:178	Cissus vitiginea	163:178	Cissus vitiginea	163:178	In this study, a new natural fiber obtained from the stem of Cissus vitiginea has been studied for the first time.
32750473	0	24	theme	novel	20:24	arg1	fiber					45:49	novel natural cellulosic fiber	20:49	novel natural cellulosic fiber	20:49	Characterization of novel natural cellulosic fiber extracted from the stem of Cissus vitiginea plant.
32750473	6	25	theme	fiber	928:932	arg1	stability					911:919	The thermal stability	899:919	The thermal stability of the fiber	899:932	The thermal stability of the fiber was found to be 304 °C with 68.72 kJ/mol kinetic activation energy.
32750473	3	26	theme	magnetic	595:602	arg1	spectroscopy					613:624	nuclear magnetic response spectroscopy	587:624	nuclear magnetic response spectroscopy	587:624	Various chemical groups distributed over the fiber surface were predicted and reported with the help of Fourier transform infrared spectroscope and nuclear magnetic response spectroscopy.
32750473	2	27	theme	proposed	265:272	arg1	fiber					274:278	the proposed fiber	261:278	the proposed fiber	261:278	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	1	28	theme	vitiginea	170:178	arg1	stem					155:158	the stem	151:158	the stem of Cissus vitiginea	151:178	In this study, a new natural fiber obtained from the stem of Cissus vitiginea has been studied for the first time.
32750473	0	29	theme	fiber	45:49	arg1	Characterization					0:15	Characterization	0:15	Characterization of novel natural cellulosic fiber	0:49	Characterization of novel natural cellulosic fiber extracted from the stem of Cissus vitiginea plant.
32750473	5	30	theme	electron	878:885	arg1	microscope					887:896	a scanning electron microscope	867:896	a scanning electron microscope	867:896	Morphological study was conducted on the fiber using a scanning electron microscope.
32750473	3	31	theme	response	604:611	arg1	spectroscopy					613:624	nuclear magnetic response spectroscopy	587:624	nuclear magnetic response spectroscopy	587:624	Various chemical groups distributed over the fiber surface were predicted and reported with the help of Fourier transform infrared spectroscope and nuclear magnetic response spectroscopy.
32750473	2	32	theme	compounds	359:367	arg1	quantity					333:340	lower quantity	327:340	lower quantity of hemicellulose compounds (14.61 wt%)	327:379	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	32	theme	compounds	359:367	arg1	%					378:378	14.61 wt%	370:378	14.61 wt%	370:378	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	32	theme	compounds	359:367	arg1	cellulose					301:309	cellulose	301:309	cellulose (65.43 wt%)	301:321	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	32	theme	compounds	359:367	arg1	%					320:320	65.43 wt%	312:320	65.43 wt%	312:320	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	0	33	theme	cellulosic	34:43	arg1	fiber					45:49	novel natural cellulosic fiber	20:49	novel natural cellulosic fiber	20:49	Characterization of novel natural cellulosic fiber extracted from the stem of Cissus vitiginea plant.
32750473	6	34	theme	kinetic	975:981	arg1	energy					994:999	68.72 kJ/mol kinetic activation energy	962:999	68.72 kJ/mol kinetic activation energy	962:999	The thermal stability of the fiber was found to be 304 °C with 68.72 kJ/mol kinetic activation energy.
32750473	2	35	theme	hemicellulose	345:357	arg1	compounds					359:367	hemicellulose compounds	345:367	hemicellulose compounds	345:367	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	7	36	theme	C.	1008:1009	arg1	reinforcement					1047:1059	reinforcement	1047:1059	reinforcement	1047:1059	Hence C. vitiginea fiber can be suggested as reinforcement for the thermoplastic green composite.
32750473	7	36	theme	C.	1008:1009	arg1	fiber					1021:1025	C. vitiginea fiber	1008:1025	C. vitiginea fiber	1008:1025	Hence C. vitiginea fiber can be suggested as reinforcement for the thermoplastic green composite.
32750473	3	37	theme	nuclear	587:593	arg1	spectroscopy					613:624	nuclear magnetic response spectroscopy	587:624	nuclear magnetic response spectroscopy	587:624	Various chemical groups distributed over the fiber surface were predicted and reported with the help of Fourier transform infrared spectroscope and nuclear magnetic response spectroscopy.
32750473	4	38	theme	12.69 nm	787:794	arg1	size					808:811	12.69 nm crystallite size	787:811	12.69 nm crystallite size	787:811	The crystalline nature of the fiber surface was examined using X-ray diffraction spectroscopy and the crystallinity index value was calculated to be 30.5% with 12.69 nm crystallite size.
32750473	2	39	theme	cellulose	301:309	arg1	quantity					333:340	lower quantity	327:340	lower quantity of hemicellulose compounds (14.61 wt%)	327:379	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	39	theme	cellulose	301:309	arg1	%					378:378	14.61 wt%	370:378	14.61 wt%	370:378	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	39	theme	cellulose	301:309	arg1	cellulose					301:309	cellulose	301:309	cellulose (65.43 wt%)	301:321	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	39	theme	cellulose	301:309	arg1	%					320:320	65.43 wt%	312:320	65.43 wt%	312:320	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	39	theme	cellulose	301:309	arg1	amount					291:296	a rich amount	284:296	a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics	284:436	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	40	contain	has	280:282	arg1	fiber					274:278	the proposed fiber	261:278	the proposed fiber	261:278	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	40	contain	has	280:282	arg2	amount					291:296	a rich amount	284:296	a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics	284:436	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	40	contain	has	280:282	arg2	cellulose					301:309	cellulose	301:309	cellulose (65.43 wt%)	301:321	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	40	contain	has	280:282	arg2	quantity					333:340	lower quantity	327:340	lower quantity of hemicellulose compounds (14.61 wt%)	327:379	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	40	contain	has	280:282	arg2	%					378:378	14.61 wt%	370:378	14.61 wt%	370:378	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	40	contain	has	280:282	arg2	%					320:320	65.43 wt%	312:320	65.43 wt%	312:320	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	7	41	theme	vitiginea	1011:1019	arg1	reinforcement					1047:1059	reinforcement	1047:1059	reinforcement	1047:1059	Hence C. vitiginea fiber can be suggested as reinforcement for the thermoplastic green composite.
32750473	7	41	theme	vitiginea	1011:1019	arg1	fiber					1021:1025	C. vitiginea fiber	1008:1025	C. vitiginea fiber	1008:1025	Hence C. vitiginea fiber can be suggested as reinforcement for the thermoplastic green composite.
32750473	4	42	theme	surface	663:669	arg1	nature					643:648	The crystalline nature	627:648	The crystalline nature of the fiber surface	627:669	The crystalline nature of the fiber surface was examined using X-ray diffraction spectroscopy and the crystallinity index value was calculated to be 30.5% with 12.69 nm crystallite size.
32750473	2	43	theme	composition	226:236	arg1	results					238:244	Chemical composition results	217:244	Chemical composition results	217:244	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	44	theme	Chemical	217:224	arg1	results					238:244	Chemical composition results	217:244	Chemical composition results	217:244	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	45	theme	rich	286:289	arg1	quantity					333:340	lower quantity	327:340	lower quantity of hemicellulose compounds (14.61 wt%)	327:379	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	45	theme	rich	286:289	arg1	%					378:378	14.61 wt%	370:378	14.61 wt%	370:378	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	45	theme	rich	286:289	arg1	cellulose					301:309	cellulose	301:309	cellulose (65.43 wt%)	301:321	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	45	theme	rich	286:289	arg1	%					320:320	65.43 wt%	312:320	65.43 wt%	312:320	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	2	45	theme	rich	286:289	arg1	amount					291:296	a rich amount	284:296	a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics	284:436	Chemical composition results confirmed that the proposed fiber has a rich amount of cellulose (65.43 wt%) and lower quantity of hemicellulose compounds (14.61 wt%),which in turn leads to better mechanical characteristics.
32750473	6	46	theme	activation	983:992	arg1	energy					994:999	68.72 kJ/mol kinetic activation energy	962:999	68.72 kJ/mol kinetic activation energy	962:999	The thermal stability of the fiber was found to be 304 °C with 68.72 kJ/mol kinetic activation energy.
32750473	5	47	theme	Morphological	814:826	arg1	study					828:832	Morphological study	814:832	Morphological study	814:832	Morphological study was conducted on the fiber using a scanning electron microscope.
32750473	3	48	dep	Fourier	543:549	arg1	transform					551:559	transform	551:559	transform infrared spectroscope and nuclear magnetic response spectroscopy	551:624	Various chemical groups distributed over the fiber surface were predicted and reported with the help of Fourier transform infrared spectroscope and nuclear magnetic response spectroscopy.
32750473	1	49	theme	new	119:121	arg1	fiber					131:135	a new natural fiber	117:135	a new natural fiber obtained from the stem of Cissus vitiginea	117:178	In this study, a new natural fiber obtained from the stem of Cissus vitiginea has been studied for the first time.
32750473	3	50	dep	the	531:533	arg1	help					535:538	help	535:538	help	535:538	Various chemical groups distributed over the fiber surface were predicted and reported with the help of Fourier transform infrared spectroscope and nuclear magnetic response spectroscopy.
32750473	4	51	with	%	780:780	arg1	size					808:811	12.69 nm crystallite size	787:811	12.69 nm crystallite size	787:811	The crystalline nature of the fiber surface was examined using X-ray diffraction spectroscopy and the crystallinity index value was calculated to be 30.5% with 12.69 nm crystallite size.
32750473	6	52	theme	68.72 kJ/mol	962:973	arg1	energy					994:999	68.72 kJ/mol kinetic activation energy	962:999	68.72 kJ/mol kinetic activation energy	962:999	The thermal stability of the fiber was found to be 304 °C with 68.72 kJ/mol kinetic activation energy.
32750473	1	53	theme	natural	123:129	arg1	fiber					131:135	a new natural fiber	117:135	a new natural fiber obtained from the stem of Cissus vitiginea	117:178	In this study, a new natural fiber obtained from the stem of Cissus vitiginea has been studied for the first time.
32750473	1	54	theme	first	205:209	arg1	time					211:214	the first time	201:214	the first time	201:214	In this study, a new natural fiber obtained from the stem of Cissus vitiginea has been studied for the first time.
34051257	7	0	theme	scanning	1208:1215	arg1	calorimetry					1217:1227	differential scanning calorimetry	1195:1227	differential scanning calorimetry (DSC)	1195:1233	The structure and morphology of the materials were characterized by scanning electron microscope (SEM), infrared spectroscopy (FTIR), X-ray diffraction (XRD), and differential scanning calorimetry (DSC).
34051257	7	0	theme	scanning	1208:1215	arg1	DSC					1230:1232	DSC	1230:1232	DSC	1230:1232	The structure and morphology of the materials were characterized by scanning electron microscope (SEM), infrared spectroscopy (FTIR), X-ray diffraction (XRD), and differential scanning calorimetry (DSC).
34051257	7	1	theme	infrared	1136:1143	arg1	FTIR					1159:1162	FTIR	1159:1162	FTIR	1159:1162	The structure and morphology of the materials were characterized by scanning electron microscope (SEM), infrared spectroscopy (FTIR), X-ray diffraction (XRD), and differential scanning calorimetry (DSC).
34051257	7	1	theme	infrared	1136:1143	arg1	spectroscopy					1145:1156	infrared spectroscopy	1136:1156	infrared spectroscopy (FTIR)	1136:1163	The structure and morphology of the materials were characterized by scanning electron microscope (SEM), infrared spectroscopy (FTIR), X-ray diffraction (XRD), and differential scanning calorimetry (DSC).
34051257	3	2	from	well-dispersed	436:449	arg1	matrix					458:463	the matrix	454:463	the matrix	454:463	The fillers were well-dispersed in the matrix and the composites exhibited improved functional characteristics such as increased thermal stability and mechanical properties.
34051257	2	3	theme	peel	347:350	arg1	agent					412:416	a proper dispersion-providing agent	382:416	a proper dispersion-providing agent	382:416	The nanoparticles were prepared via a green way using the pectin-based banana peel extract as the stabilizer and a proper dispersion-providing agent.
34051257	2	3	theme	peel	347:350	arg1	extract					352:358	the pectin-based banana peel extract	323:358	the pectin-based banana peel extract	323:358	The nanoparticles were prepared via a green way using the pectin-based banana peel extract as the stabilizer and a proper dispersion-providing agent.
34051257	2	3	theme	peel	347:350	arg1	stabilizer					367:376	the stabilizer	363:376	the stabilizer	363:376	The nanoparticles were prepared via a green way using the pectin-based banana peel extract as the stabilizer and a proper dispersion-providing agent.
34051257	7	4	theme	scanning	1100:1107	arg1	SEM					1130:1132	SEM	1130:1132	SEM	1130:1132	The structure and morphology of the materials were characterized by scanning electron microscope (SEM), infrared spectroscopy (FTIR), X-ray diffraction (XRD), and differential scanning calorimetry (DSC).
34051257	7	4	theme	scanning	1100:1107	arg1	microscope					1118:1127	scanning electron microscope	1100:1127	scanning electron microscope (SEM)	1100:1133	The structure and morphology of the materials were characterized by scanning electron microscope (SEM), infrared spectroscopy (FTIR), X-ray diffraction (XRD), and differential scanning calorimetry (DSC).
34051257	2	5	theme	banana	340:345	arg1	agent					412:416	a proper dispersion-providing agent	382:416	a proper dispersion-providing agent	382:416	The nanoparticles were prepared via a green way using the pectin-based banana peel extract as the stabilizer and a proper dispersion-providing agent.
34051257	2	5	theme	banana	340:345	arg1	extract					352:358	the pectin-based banana peel extract	323:358	the pectin-based banana peel extract	323:358	The nanoparticles were prepared via a green way using the pectin-based banana peel extract as the stabilizer and a proper dispersion-providing agent.
34051257	2	5	theme	banana	340:345	arg1	stabilizer					367:376	the stabilizer	363:376	the stabilizer	363:376	The nanoparticles were prepared via a green way using the pectin-based banana peel extract as the stabilizer and a proper dispersion-providing agent.
34051257	10	6	theme	used	1460:1463	arg1	biopolymer					1465:1474	The used biopolymer	1456:1474	The used biopolymer	1456:1474	The used biopolymer reacts with ZnO via ionic interaction and through hydrogen bond in the case of HDPE.
34051257	1	7	theme	injection	250:258	arg1	molding					260:266	injection molding	250:266	injection molding	250:266	A series of nanocomposites made of high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles (ZnO NPs) were produced by extrusion and injection molding.
34051257	7	8	theme	differential	1195:1206	arg1	calorimetry					1217:1227	differential scanning calorimetry	1195:1227	differential scanning calorimetry (DSC)	1195:1233	The structure and morphology of the materials were characterized by scanning electron microscope (SEM), infrared spectroscopy (FTIR), X-ray diffraction (XRD), and differential scanning calorimetry (DSC).
34051257	7	8	theme	differential	1195:1206	arg1	DSC					1230:1232	DSC	1230:1232	DSC	1230:1232	The structure and morphology of the materials were characterized by scanning electron microscope (SEM), infrared spectroscopy (FTIR), X-ray diffraction (XRD), and differential scanning calorimetry (DSC).
34051257	6	9	theme	composites	826:835	arg1	properties					808:817	The fire properties	799:817	The fire properties of the composites	799:835	The fire properties of the composites were enhanced as the amount of the gas products produced during their degradation was reduced, what was confirmed by thermogravimetric analysis coupled with gas products analyses (TGA/FTIR/QMS).
34051257	3	10	theme	functional	503:512	arg1	stability					556:564	increased thermal stability	538:564	increased thermal stability	538:564	The fillers were well-dispersed in the matrix and the composites exhibited improved functional characteristics such as increased thermal stability and mechanical properties.
34051257	3	10	theme	functional	503:512	arg1	characteristics					514:528	improved functional characteristics	494:528	improved functional characteristics such as increased thermal stability and mechanical properties	494:590	The fillers were well-dispersed in the matrix and the composites exhibited improved functional characteristics such as increased thermal stability and mechanical properties.
34051257	3	10	theme	functional	503:512	arg1	properties					581:590	mechanical properties	570:590	mechanical properties	570:590	The fillers were well-dispersed in the matrix and the composites exhibited improved functional characteristics such as increased thermal stability and mechanical properties.
34051257	4	11	theme	crystallization	674:688	arg1	process					690:696	the crystallization process	670:696	the crystallization process of HDPE	670:704	The presence of the pectin-organophilized filler had a significant impact on the crystallization process of HDPE.
34051257	3	12	theme	increased	538:546	arg1	stability					556:564	increased thermal stability	538:564	increased thermal stability	538:564	The fillers were well-dispersed in the matrix and the composites exhibited improved functional characteristics such as increased thermal stability and mechanical properties.
34051257	1	13	theme	wt	180:181	arg1	oxide					189:193	10 wt% zinc oxide	177:193	10 wt% zinc oxide	177:193	A series of nanocomposites made of high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles (ZnO NPs) were produced by extrusion and injection molding.
34051257	9	14	theme	final	1439:1443	arg1	composite					1445:1453	the final composite	1435:1453	the final composite	1435:1453	An in-depth analysis revealed that the HDPE-pectin-ZnO interactions are crucial for the structural and performance properties of the final composite.
34051257	9	15	theme	in-depth	1309:1316	arg1	analysis					1318:1325	An in-depth analysis	1306:1325	An in-depth analysis	1306:1325	An in-depth analysis revealed that the HDPE-pectin-ZnO interactions are crucial for the structural and performance properties of the final composite.
34051257	3	16	theme	thermal	548:554	arg1	stability					556:564	increased thermal stability	538:564	increased thermal stability	538:564	The fillers were well-dispersed in the matrix and the composites exhibited improved functional characteristics such as increased thermal stability and mechanical properties.
34051257	7	17	dep	structure	1036:1044	arg1	The					1032:1034	The	1032:1034	The	1032:1034	The structure and morphology of the materials were characterized by scanning electron microscope (SEM), infrared spectroscopy (FTIR), X-ray diffraction (XRD), and differential scanning calorimetry (DSC).
34051257	1	18	theme	%	182:182	arg1	oxide					189:193	10 wt% zinc oxide	177:193	10 wt% zinc oxide	177:193	A series of nanocomposites made of high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles (ZnO NPs) were produced by extrusion and injection molding.
34051257	9	19	theme	HDPE-pectin-ZnO	1345:1359	arg1	crucial					1378:1384	crucial	1378:1384	crucial	1378:1384	An in-depth analysis revealed that the HDPE-pectin-ZnO interactions are crucial for the structural and performance properties of the final composite.
34051257	9	19	theme	HDPE-pectin-ZnO	1345:1359	arg1	interactions					1361:1372	the HDPE-pectin-ZnO interactions	1341:1372	the HDPE-pectin-ZnO interactions	1341:1372	An in-depth analysis revealed that the HDPE-pectin-ZnO interactions are crucial for the structural and performance properties of the final composite.
34051257	7	20	theme	electron	1109:1116	arg1	SEM					1130:1132	SEM	1130:1132	SEM	1130:1132	The structure and morphology of the materials were characterized by scanning electron microscope (SEM), infrared spectroscopy (FTIR), X-ray diffraction (XRD), and differential scanning calorimetry (DSC).
34051257	7	20	theme	electron	1109:1116	arg1	microscope					1118:1127	scanning electron microscope	1100:1127	scanning electron microscope (SEM)	1100:1133	The structure and morphology of the materials were characterized by scanning electron microscope (SEM), infrared spectroscopy (FTIR), X-ray diffraction (XRD), and differential scanning calorimetry (DSC).
34051257	10	21	theme	HDPE	1555:1558	arg1	case					1547:1550	the case	1543:1550	the case of HDPE	1543:1558	The used biopolymer reacts with ZnO via ionic interaction and through hydrogen bond in the case of HDPE.
34051257	2	22	theme	green	307:311	arg1	way					313:315	a green way	305:315	a green way using the pectin-based banana peel extract as the stabilizer and a proper dispersion-providing agent	305:416	The nanoparticles were prepared via a green way using the pectin-based banana peel extract as the stabilizer and a proper dispersion-providing agent.
34051257	1	23	theme	zinc	184:187	arg1	oxide					189:193	10 wt% zinc oxide	177:193	10 wt% zinc oxide	177:193	A series of nanocomposites made of high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles (ZnO NPs) were produced by extrusion and injection molding.
34051257	0	24	theme	Pectin-organophilized	0:20	arg1	ZnO					22:24	Pectin-organophilized ZnO	0:24	Pectin-organophilized ZnO	0:24	Pectin-organophilized ZnO nanoparticles as sustainable fillers for high-density polyethylene composites.
34051257	6	25	theme	products	998:1005	arg1	TGA/FTIR/QMS					1017:1028	TGA/FTIR/QMS	1017:1028	TGA/FTIR/QMS	1017:1028	The fire properties of the composites were enhanced as the amount of the gas products produced during their degradation was reduced, what was confirmed by thermogravimetric analysis coupled with gas products analyses (TGA/FTIR/QMS).
34051257	6	25	theme	products	998:1005	arg1	analyses					1007:1014	gas products analyses	994:1014	gas products analyses (TGA/FTIR/QMS)	994:1029	The fire properties of the composites were enhanced as the amount of the gas products produced during their degradation was reduced, what was confirmed by thermogravimetric analysis coupled with gas products analyses (TGA/FTIR/QMS).
34051257	6	26	theme	fire	803:806	arg1	properties					808:817	The fire properties	799:817	The fire properties of the composites	799:835	The fire properties of the composites were enhanced as the amount of the gas products produced during their degradation was reduced, what was confirmed by thermogravimetric analysis coupled with gas products analyses (TGA/FTIR/QMS).
34051257	1	27	theme	oxide	189:193	arg1	nanoparticles					195:207	high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles	140:207	high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles (ZnO NPs)	140:217	A series of nanocomposites made of high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles (ZnO NPs) were produced by extrusion and injection molding.
34051257	1	27	theme	oxide	189:193	arg1	NPs					214:216	ZnO NPs	210:216	ZnO NPs	210:216	A series of nanocomposites made of high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles (ZnO NPs) were produced by extrusion and injection molding.
34051257	7	28	theme	X-ray	1166:1170	arg1	diffraction					1172:1182	X-ray diffraction	1166:1182	X-ray diffraction (XRD)	1166:1188	The structure and morphology of the materials were characterized by scanning electron microscope (SEM), infrared spectroscopy (FTIR), X-ray diffraction (XRD), and differential scanning calorimetry (DSC).
34051257	7	28	theme	X-ray	1166:1170	arg1	XRD					1185:1187	XRD	1185:1187	XRD	1185:1187	The structure and morphology of the materials were characterized by scanning electron microscope (SEM), infrared spectroscopy (FTIR), X-ray diffraction (XRD), and differential scanning calorimetry (DSC).
34051257	6	29	theme	gas	994:996	arg1	TGA/FTIR/QMS					1017:1028	TGA/FTIR/QMS	1017:1028	TGA/FTIR/QMS	1017:1028	The fire properties of the composites were enhanced as the amount of the gas products produced during their degradation was reduced, what was confirmed by thermogravimetric analysis coupled with gas products analyses (TGA/FTIR/QMS).
34051257	6	29	theme	gas	994:996	arg1	analyses					1007:1014	gas products analyses	994:1014	gas products analyses (TGA/FTIR/QMS)	994:1029	The fire properties of the composites were enhanced as the amount of the gas products produced during their degradation was reduced, what was confirmed by thermogravimetric analysis coupled with gas products analyses (TGA/FTIR/QMS).
34051257	5	30	theme	degradation	727:737	arg1	process					739:745	the degradation process	723:745	the degradation process	723:745	The kinetics of the degradation process was also altered in comparison to the pure polymer.
34051257	9	31	theme	composite	1445:1453	arg1	properties					1421:1430	the structural and performance properties	1390:1430	properties	1421:1430	An in-depth analysis revealed that the HDPE-pectin-ZnO interactions are crucial for the structural and performance properties of the final composite.
34051257	1	32	theme	nanocomposites	117:130	arg1	series					107:112	A series	105:112	A series of nanocomposites made of high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles (ZnO NPs)	105:217	A series of nanocomposites made of high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles (ZnO NPs) were produced by extrusion and injection molding.
34051257	8	33	theme	mechanical	1254:1263	arg1	properties					1265:1274	the mechanical properties	1250:1274	the mechanical properties	1250:1274	Additionally, the mechanical properties were tested by tensile tests.
34051257	5	34	theme	process	739:745	arg1	kinetics					711:718	The kinetics	707:718	The kinetics of the degradation process	707:745	The kinetics of the degradation process was also altered in comparison to the pure polymer.
34051257	1	35	theme	10	177:178	arg1	wt					180:181	wt	180:181	wt	180:181	A series of nanocomposites made of high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles (ZnO NPs) were produced by extrusion and injection molding.
34051257	3	36	theme	improved	494:501	arg1	stability					556:564	increased thermal stability	538:564	increased thermal stability	538:564	The fillers were well-dispersed in the matrix and the composites exhibited improved functional characteristics such as increased thermal stability and mechanical properties.
34051257	3	36	theme	improved	494:501	arg1	characteristics					514:528	improved functional characteristics	494:528	improved functional characteristics such as increased thermal stability and mechanical properties	494:590	The fillers were well-dispersed in the matrix and the composites exhibited improved functional characteristics such as increased thermal stability and mechanical properties.
34051257	3	36	theme	improved	494:501	arg1	properties					581:590	mechanical properties	570:590	mechanical properties	570:590	The fillers were well-dispersed in the matrix and the composites exhibited improved functional characteristics such as increased thermal stability and mechanical properties.
34051257	10	37	theme	ionic	1496:1500	arg1	interaction					1502:1512	ionic interaction	1496:1512	ionic interaction	1496:1512	The used biopolymer reacts with ZnO via ionic interaction and through hydrogen bond in the case of HDPE.
34051257	2	38	theme	dispersion-providing	391:410	arg1	extract					352:358	the pectin-based banana peel extract	323:358	the pectin-based banana peel extract	323:358	The nanoparticles were prepared via a green way using the pectin-based banana peel extract as the stabilizer and a proper dispersion-providing agent.
34051257	2	38	theme	dispersion-providing	391:410	arg1	agent					412:416	a proper dispersion-providing agent	382:416	a proper dispersion-providing agent	382:416	The nanoparticles were prepared via a green way using the pectin-based banana peel extract as the stabilizer and a proper dispersion-providing agent.
34051257	4	39	theme	HDPE	701:704	arg1	process					690:696	the crystallization process	670:696	the crystallization process of HDPE	670:704	The presence of the pectin-organophilized filler had a significant impact on the crystallization process of HDPE.
34051257	1	40	theme	ZnO	210:212	arg1	nanoparticles					195:207	high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles	140:207	high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles (ZnO NPs)	140:217	A series of nanocomposites made of high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles (ZnO NPs) were produced by extrusion and injection molding.
34051257	1	40	theme	ZnO	210:212	arg1	NPs					214:216	ZnO NPs	210:216	ZnO NPs	210:216	A series of nanocomposites made of high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles (ZnO NPs) were produced by extrusion and injection molding.
34051257	0	41	theme	sustainable	43:53	arg1	fillers					55:61	sustainable fillers	43:61	sustainable fillers for high-density polyethylene composites	43:102	Pectin-organophilized ZnO nanoparticles as sustainable fillers for high-density polyethylene composites.
34051257	6	42	theme	gas	872:874	arg1	products					876:883	the gas products	868:883	the gas products	868:883	The fire properties of the composites were enhanced as the amount of the gas products produced during their degradation was reduced, what was confirmed by thermogravimetric analysis coupled with gas products analyses (TGA/FTIR/QMS).
34051257	2	43	theme	proper	384:389	arg1	extract					352:358	the pectin-based banana peel extract	323:358	the pectin-based banana peel extract	323:358	The nanoparticles were prepared via a green way using the pectin-based banana peel extract as the stabilizer and a proper dispersion-providing agent.
34051257	2	43	theme	proper	384:389	arg1	agent					412:416	a proper dispersion-providing agent	382:416	a proper dispersion-providing agent	382:416	The nanoparticles were prepared via a green way using the pectin-based banana peel extract as the stabilizer and a proper dispersion-providing agent.
34051257	0	44	theme	high-density	67:78	arg1	composites					93:102	high-density polyethylene composites	67:102	high-density polyethylene composites	67:102	Pectin-organophilized ZnO nanoparticles as sustainable fillers for high-density polyethylene composites.
34051257	6	45	theme	thermogravimetric	954:970	arg1	analysis					972:979	thermogravimetric analysis	954:979	thermogravimetric analysis coupled with gas products analyses (TGA/FTIR/QMS)	954:1029	The fire properties of the composites were enhanced as the amount of the gas products produced during their degradation was reduced, what was confirmed by thermogravimetric analysis coupled with gas products analyses (TGA/FTIR/QMS).
34051257	5	46	theme	pure	785:788	arg1	polymer					790:796	the pure polymer	781:796	the pure polymer	781:796	The kinetics of the degradation process was also altered in comparison to the pure polymer.
34051257	2	47	theme	pectin-based	327:338	arg1	agent					412:416	a proper dispersion-providing agent	382:416	a proper dispersion-providing agent	382:416	The nanoparticles were prepared via a green way using the pectin-based banana peel extract as the stabilizer and a proper dispersion-providing agent.
34051257	2	47	theme	pectin-based	327:338	arg1	extract					352:358	the pectin-based banana peel extract	323:358	the pectin-based banana peel extract	323:358	The nanoparticles were prepared via a green way using the pectin-based banana peel extract as the stabilizer and a proper dispersion-providing agent.
34051257	2	47	theme	pectin-based	327:338	arg1	stabilizer					367:376	the stabilizer	363:376	the stabilizer	363:376	The nanoparticles were prepared via a green way using the pectin-based banana peel extract as the stabilizer and a proper dispersion-providing agent.
34051257	10	48	from	bond	1535:1538	arg1	case					1547:1550	the case	1543:1550	the case of HDPE	1543:1558	The used biopolymer reacts with ZnO via ionic interaction and through hydrogen bond in the case of HDPE.
34051257	7	49	theme	materials	1068:1076	arg1	morphology					1050:1059	morphology	1050:1059	morphology	1050:1059	The structure and morphology of the materials were characterized by scanning electron microscope (SEM), infrared spectroscopy (FTIR), X-ray diffraction (XRD), and differential scanning calorimetry (DSC).
34051257	7	49	theme	materials	1068:1076	arg1	structure					1036:1044	structure	1036:1044	structure	1036:1044	The structure and morphology of the materials were characterized by scanning electron microscope (SEM), infrared spectroscopy (FTIR), X-ray diffraction (XRD), and differential scanning calorimetry (DSC).
34051257	4	50	theme	pectin-organophilized	613:633	arg1	filler					635:640	the pectin-organophilized filler	609:640	the pectin-organophilized filler	609:640	The presence of the pectin-organophilized filler had a significant impact on the crystallization process of HDPE.
34051257	3	51	from	matrix	458:463	arg1	well-dispersed					436:449	well-dispersed	436:449	well-dispersed	436:449	The fillers were well-dispersed in the matrix and the composites exhibited improved functional characteristics such as increased thermal stability and mechanical properties.
34051257	3	51	from	matrix	458:463	arg1	fillers					423:429	The fillers	419:429	The fillers	419:429	The fillers were well-dispersed in the matrix and the composites exhibited improved functional characteristics such as increased thermal stability and mechanical properties.
34051257	1	52	theme	high-density	140:151	arg1	polyethylene					153:164	high-density polyethylene	140:164	high-density polyethylene (HDPE)	140:171	A series of nanocomposites made of high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles (ZnO NPs) were produced by extrusion and injection molding.
34051257	1	52	theme	high-density	140:151	arg1	HDPE					167:170	HDPE	167:170	HDPE	167:170	A series of nanocomposites made of high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles (ZnO NPs) were produced by extrusion and injection molding.
34051257	10	53	theme	hydrogen	1526:1533	arg1	bond					1535:1538	hydrogen bond	1526:1538	hydrogen bond in the case of HDPE	1526:1558	The used biopolymer reacts with ZnO via ionic interaction and through hydrogen bond in the case of HDPE.
34051257	1	54	theme	polyethylene	153:164	arg1	nanoparticles					195:207	high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles	140:207	high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles (ZnO NPs)	140:217	A series of nanocomposites made of high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles (ZnO NPs) were produced by extrusion and injection molding.
34051257	1	54	theme	polyethylene	153:164	arg1	NPs					214:216	ZnO NPs	210:216	ZnO NPs	210:216	A series of nanocomposites made of high-density polyethylene (HDPE) and 10 wt% zinc oxide nanoparticles (ZnO NPs) were produced by extrusion and injection molding.
34051257	3	55	theme	mechanical	570:579	arg1	properties					581:590	mechanical properties	570:590	mechanical properties	570:590	The fillers were well-dispersed in the matrix and the composites exhibited improved functional characteristics such as increased thermal stability and mechanical properties.
34051257	9	56	theme	structural	1394:1403	arg1	properties					1421:1430	the structural and performance properties	1390:1430	properties	1421:1430	An in-depth analysis revealed that the HDPE-pectin-ZnO interactions are crucial for the structural and performance properties of the final composite.
34051257	8	57	theme	tensile	1291:1297	arg1	tests					1299:1303	tensile tests	1291:1303	tensile tests	1291:1303	Additionally, the mechanical properties were tested by tensile tests.
34051257	4	58	theme	filler	635:640	arg1	presence					597:604	The presence	593:604	The presence of the pectin-organophilized filler	593:640	The presence of the pectin-organophilized filler had a significant impact on the crystallization process of HDPE.
34051257	6	59	theme	products	876:883	arg1	amount					858:863	the amount	854:863	the amount of the gas products produced during their degradation	854:917	The fire properties of the composites were enhanced as the amount of the gas products produced during their degradation was reduced, what was confirmed by thermogravimetric analysis coupled with gas products analyses (TGA/FTIR/QMS).
34051257	6	59	theme	products	876:883	arg1	products					876:883	the gas products	868:883	the gas products	868:883	The fire properties of the composites were enhanced as the amount of the gas products produced during their degradation was reduced, what was confirmed by thermogravimetric analysis coupled with gas products analyses (TGA/FTIR/QMS).
34051257	4	60	theme	significant	648:658	arg1	impact					660:665	a significant impact	646:665	a significant impact	646:665	The presence of the pectin-organophilized filler had a significant impact on the crystallization process of HDPE.
34051257	0	61	theme	polyethylene	80:91	arg1	composites					93:102	high-density polyethylene composites	67:102	high-density polyethylene composites	67:102	Pectin-organophilized ZnO nanoparticles as sustainable fillers for high-density polyethylene composites.
34051257	4	62	contain	had	642:644	arg2	impact					660:665	a significant impact	646:665	a significant impact	646:665	The presence of the pectin-organophilized filler had a significant impact on the crystallization process of HDPE.
34051257	4	62	contain	had	642:644	arg1	presence					597:604	The presence	593:604	The presence of the pectin-organophilized filler	593:640	The presence of the pectin-organophilized filler had a significant impact on the crystallization process of HDPE.
34051257	9	63	theme	performance	1409:1419	arg1	properties					1421:1430	the structural and performance properties	1390:1430	properties	1421:1430	An in-depth analysis revealed that the HDPE-pectin-ZnO interactions are crucial for the structural and performance properties of the final composite.
34940684	7	0	theme	different	1110:1118	arg1	variants					1127:1134	different mutant variants	1110:1134	different mutant variants	1110:1134	RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants) was studied using surface plasmon resonance (SPR).
34940684	5	1	dep	Monostroma	704:713	arg1	nitidum					715:721	Monostroma nitidum	704:721	Monostroma nitidum	704:721	In the current study, rhamnan sulfate (RS), a polysaccharide with a rhamnose backbone from a green seaweed, Monostroma nitidum, was evaluated for binding to the S-protein from SARS-CoV-2 and inhibition of viral infectivity in vitro.
34940684	3	2	from	sulfate	387:393	arg1	surface					407:413	the surface	403:413	the surface of host cells	403:427	In addition to ACE2, heparan sulfate (HS) on the surface of host cells also plays a significant role as a co-receptor.
34940684	2	3	dep	spike	273:277	arg1	S					280:280	S	280:280	S	280:280	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	5	4	theme	rhamnan	618:624	arg1	RS					635:636	RS	635:636	RS	635:636	In the current study, rhamnan sulfate (RS), a polysaccharide with a rhamnose backbone from a green seaweed, Monostroma nitidum, was evaluated for binding to the S-protein from SARS-CoV-2 and inhibition of viral infectivity in vitro.
34940684	5	4	theme	rhamnan	618:624	arg1	sulfate					626:632	rhamnan sulfate	618:632	rhamnan sulfate (RS)	618:637	In the current study, rhamnan sulfate (RS), a polysaccharide with a rhamnose backbone from a green seaweed, Monostroma nitidum, was evaluated for binding to the S-protein from SARS-CoV-2 and inhibition of viral infectivity in vitro.
34940684	5	4	theme	rhamnan	618:624	arg1	polysaccharide					642:655	a polysaccharide	640:655	a polysaccharide with a rhamnose backbone from a green seaweed, Monostroma nitidum	640:721	In the current study, rhamnan sulfate (RS), a polysaccharide with a rhamnose backbone from a green seaweed, Monostroma nitidum, was evaluated for binding to the S-protein from SARS-CoV-2 and inhibition of viral infectivity in vitro.
34940684	7	5	theme	sulfated	1043:1050	arg1	HS					1052:1053	a highly sulfated HS	1034:1053	a highly sulfated HS	1034:1053	RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants) was studied using surface plasmon resonance (SPR).
34940684	7	5	theme	sulfated	1043:1050	arg1	heparin					1025:1031	heparin	1025:1031	heparin	1025:1031	RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants) was studied using surface plasmon resonance (SPR).
34940684	7	6	with	interaction	1010:1020	arg1	protein					1082:1088	the SARS-CoV-2 spike protein	1061:1088	the SARS-CoV-2 spike protein (from wild type and different mutant variants)	1061:1135	RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants) was studied using surface plasmon resonance (SPR).
34940684	8	7	theme	binding	1203:1209	arg1	studies					1211:1217	competitive binding studies	1191:1217	competitive binding studies	1191:1217	In competitive binding studies, the IC50 of RS against the S-protein receptor binding domain (RBD) binding to immobilized heparin was 1.6 ng/mL, which is much lower than the IC50 for heparin (~750 ng/mL).
34940684	8	8	theme	receptor	1257:1264	arg1	RBD					1282:1284	RBD	1282:1284	RBD	1282:1284	In competitive binding studies, the IC50 of RS against the S-protein receptor binding domain (RBD) binding to immobilized heparin was 1.6 ng/mL, which is much lower than the IC50 for heparin (~750 ng/mL).
34940684	8	8	theme	receptor	1257:1264	arg1	domain					1274:1279	the S-protein receptor binding domain	1243:1279	the S-protein receptor binding domain (RBD) binding to immobilized heparin	1243:1316	In competitive binding studies, the IC50 of RS against the S-protein receptor binding domain (RBD) binding to immobilized heparin was 1.6 ng/mL, which is much lower than the IC50 for heparin (~750 ng/mL).
34940684	2	9	theme	spike	273:277	arg1	protein					283:289	its spike (S) protein	269:289	its spike (S) protein	269:289	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	4	10	theme	anti-COVID-19	570:582	arg1	activities					584:593	anti-COVID-19 activities	570:593	anti-COVID-19 activities	570:593	Our previous studies demonstrated that sulfated glycans, such as heparin and fucoidans, show anti-COVID-19 activities.
34940684	4	11	theme	sulfated	516:523	arg1	fucoidans					554:562	fucoidans	554:562	fucoidans	554:562	Our previous studies demonstrated that sulfated glycans, such as heparin and fucoidans, show anti-COVID-19 activities.
34940684	4	11	theme	sulfated	516:523	arg1	heparin					542:548	heparin	542:548	heparin	542:548	Our previous studies demonstrated that sulfated glycans, such as heparin and fucoidans, show anti-COVID-19 activities.
34940684	4	11	theme	sulfated	516:523	arg1	glycans					525:531	sulfated glycans	516:531	sulfated glycans	516:531	Our previous studies demonstrated that sulfated glycans, such as heparin and fucoidans, show anti-COVID-19 activities.
34940684	7	12	theme	plasmon	1163:1169	arg1	resonance					1171:1179	surface plasmon resonance	1155:1179	surface plasmon resonance (SPR)	1155:1185	RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants) was studied using surface plasmon resonance (SPR).
34940684	7	12	theme	plasmon	1163:1169	arg1	SPR					1182:1184	SPR	1182:1184	SPR	1182:1184	RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants) was studied using surface plasmon resonance (SPR).
34940684	0	13	from	nitidum	60:66	arg1	Activity					16:23	Anti-SARS-CoV-2 Activity	0:23	Anti-SARS-CoV-2 Activity of Rhamnan Sulfate from Monostroma nitidum.	0:67	Anti-SARS-CoV-2 Activity of Rhamnan Sulfate from Monostroma nitidum.
34940684	3	14	theme	cells	423:427	arg1	surface					407:413	the surface	403:413	the surface of host cells	403:427	In addition to ACE2, heparan sulfate (HS) on the surface of host cells also plays a significant role as a co-receptor.
34940684	10	15	theme	cell-based	1538:1547	arg1	assay					1549:1553	an in vitro cell-based assay	1526:1553	an in vitro cell-based assay	1526:1553	Finally, in an in vitro cell-based assay, RS showed strong antiviral activities against wild type SARS-CoV-2 and the delta variant.
34940684	10	16	theme	in	1529:1530	arg1	assay					1549:1553	an in vitro cell-based assay	1526:1553	an in vitro cell-based assay	1526:1553	Finally, in an in vitro cell-based assay, RS showed strong antiviral activities against wild type SARS-CoV-2 and the delta variant.
34940684	2	17	theme	cell	303:306	arg1	receptor					308:315	a host cell receptor	296:315	a host cell receptor	296:315	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	2	17	theme	cell	303:306	arg1	enzyme					341:346	angiotensin-converting enzyme 2	318:348	angiotensin-converting enzyme 2 (ACE2)	318:355	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	10	18	dep	type	1607:1610	arg1	SARS-CoV-2					1612:1621	SARS-CoV-2	1612:1621	SARS-CoV-2	1612:1621	Finally, in an in vitro cell-based assay, RS showed strong antiviral activities against wild type SARS-CoV-2 and the delta variant.
34940684	5	19	from	SARS-CoV-2	772:781	arg1	S-protein					757:765	the S-protein	753:765	the S-protein from SARS-CoV-2	753:781	In the current study, rhamnan sulfate (RS), a polysaccharide with a rhamnose backbone from a green seaweed, Monostroma nitidum, was evaluated for binding to the S-protein from SARS-CoV-2 and inhibition of viral infectivity in vitro.
34940684	1	20	theme	COVID-19	73:80	arg1	pandemic					82:89	The COVID-19 pandemic	69:89	The COVID-19 pandemic	69:89	The COVID-19 pandemic is a major human health concern.
34940684	1	20	theme	COVID-19	73:80	arg1	concern					115:121	a major human health concern	94:121	a major human health concern	94:121	The COVID-19 pandemic is a major human health concern.
34940684	3	21	theme	heparan	379:385	arg1	HS					396:397	HS	396:397	HS	396:397	In addition to ACE2, heparan sulfate (HS) on the surface of host cells also plays a significant role as a co-receptor.
34940684	3	21	theme	heparan	379:385	arg1	sulfate					387:393	heparan sulfate	379:393	heparan sulfate (HS) on the surface of host cells	379:427	In addition to ACE2, heparan sulfate (HS) on the surface of host cells also plays a significant role as a co-receptor.
34940684	5	22	theme	green	689:693	arg1	Monostroma					704:713	Monostroma	704:713	Monostroma	704:713	In the current study, rhamnan sulfate (RS), a polysaccharide with a rhamnose backbone from a green seaweed, Monostroma nitidum, was evaluated for binding to the S-protein from SARS-CoV-2 and inhibition of viral infectivity in vitro.
34940684	5	22	theme	green	689:693	arg1	seaweed					695:701	a green seaweed	687:701	a green seaweed	687:701	In the current study, rhamnan sulfate (RS), a polysaccharide with a rhamnose backbone from a green seaweed, Monostroma nitidum, was evaluated for binding to the S-protein from SARS-CoV-2 and inhibition of viral infectivity in vitro.
34940684	2	23	theme	responsible	137:147	arg1	coronavirus					197:207	severe acute respiratory syndrome coronavirus 2	163:209	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	163:222	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	2	23	theme	responsible	137:147	arg1	pathogen					128:135	The pathogen	124:135	The pathogen responsible for COVID-19	124:160	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	7	24	theme	heparin	1025:1031	arg1	interaction					1010:1020	the interaction	1006:1020	the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants)	1006:1135	RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants) was studied using surface plasmon resonance (SPR).
34940684	8	25	theme	RS	1232:1233	arg1	ng/mL					1326:1330	1.6 ng/mL	1322:1330	1.6 ng/mL	1322:1330	In competitive binding studies, the IC50 of RS against the S-protein receptor binding domain (RBD) binding to immobilized heparin was 1.6 ng/mL, which is much lower than the IC50 for heparin (~750 ng/mL).
34940684	8	25	theme	RS	1232:1233	arg1	IC50					1224:1227	the IC50	1220:1227	the IC50 of RS against the S-protein receptor binding domain (RBD) binding to immobilized heparin	1220:1316	In competitive binding studies, the IC50 of RS against the S-protein receptor binding domain (RBD) binding to immobilized heparin was 1.6 ng/mL, which is much lower than the IC50 for heparin (~750 ng/mL).
34940684	8	26	theme	competitive	1191:1201	arg1	studies					1211:1217	competitive binding studies	1191:1217	competitive binding studies	1191:1217	In competitive binding studies, the IC50 of RS against the S-protein receptor binding domain (RBD) binding to immobilized heparin was 1.6 ng/mL, which is much lower than the IC50 for heparin (~750 ng/mL).
34940684	5	27	theme	rhamnose	664:671	arg1	backbone					673:680	a rhamnose backbone	662:680	a rhamnose backbone from a green seaweed, Monostroma nitidum	662:721	In the current study, rhamnan sulfate (RS), a polysaccharide with a rhamnose backbone from a green seaweed, Monostroma nitidum, was evaluated for binding to the S-protein from SARS-CoV-2 and inhibition of viral infectivity in vitro.
34940684	7	28	theme	mutant	1120:1125	arg1	variants					1127:1134	different mutant variants	1110:1134	different mutant variants	1110:1134	RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants) was studied using surface plasmon resonance (SPR).
34940684	2	29	theme	protein	283:289	arg1	interaction					254:264	the interaction	250:264	the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2)	250:355	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	7	30	from	type	1101:1104	arg1	protein					1082:1088	the SARS-CoV-2 spike protein	1061:1088	the SARS-CoV-2 spike protein (from wild type and different mutant variants)	1061:1135	RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants) was studied using surface plasmon resonance (SPR).
34940684	0	31	theme	Anti-SARS-CoV-2	0:14	arg1	Activity					16:23	Anti-SARS-CoV-2 Activity	0:23	Anti-SARS-CoV-2 Activity of Rhamnan Sulfate from Monostroma nitidum.	0:67	Anti-SARS-CoV-2 Activity of Rhamnan Sulfate from Monostroma nitidum.
34940684	9	32	theme	stronger	1403:1410	arg1	inhibition					1412:1421	stronger inhibition	1403:1421	stronger inhibition	1403:1421	RS showed stronger inhibition than heparin on the S-protein RBD or pseudoviral particles binding to immobilized heparin.
34940684	7	33	theme	interaction	1010:1020	arg1	inhibition					992:1001	RS inhibition	989:1001	RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants)	989:1135	RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants) was studied using surface plasmon resonance (SPR).
34940684	10	34	dep	in	1529:1530	arg1	vitro					1532:1536	vitro	1532:1536	vitro	1532:1536	Finally, in an in vitro cell-based assay, RS showed strong antiviral activities against wild type SARS-CoV-2 and the delta variant.
34940684	9	35	theme	immobilized	1493:1503	arg1	heparin					1505:1511	immobilized heparin	1493:1511	immobilized heparin	1493:1511	RS showed stronger inhibition than heparin on the S-protein RBD or pseudoviral particles binding to immobilized heparin.
34940684	0	36	theme	Rhamnan	28:34	arg1	Sulfate					36:42	Rhamnan Sulfate	28:42	Rhamnan Sulfate	28:42	Anti-SARS-CoV-2 Activity of Rhamnan Sulfate from Monostroma nitidum.
34940684	6	37	theme	RS	863:864	arg1	characteristics					844:858	The structural characteristics	829:858	The structural characteristics of RS	829:864	The structural characteristics of RS were investigated by determining its monosaccharide composition and performing two-dimensional nuclear magnetic resonance.
34940684	1	38	theme	major	96:100	arg1	pandemic					82:89	The COVID-19 pandemic	69:89	The COVID-19 pandemic	69:89	The COVID-19 pandemic is a major human health concern.
34940684	1	38	theme	major	96:100	arg1	concern					115:121	a major human health concern	94:121	a major human health concern	94:121	The COVID-19 pandemic is a major human health concern.
34940684	7	39	theme	spike	1076:1080	arg1	protein					1082:1088	the SARS-CoV-2 spike protein	1061:1088	the SARS-CoV-2 spike protein (from wild type and different mutant variants)	1061:1135	RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants) was studied using surface plasmon resonance (SPR).
34940684	4	40	theme	previous	481:488	arg1	studies					490:496	Our previous studies	477:496	Our previous studies	477:496	Our previous studies demonstrated that sulfated glycans, such as heparin and fucoidans, show anti-COVID-19 activities.
34940684	3	41	theme	significant	442:452	arg1	role					454:457	a significant role	440:457	a significant role	440:457	In addition to ACE2, heparan sulfate (HS) on the surface of host cells also plays a significant role as a co-receptor.
34940684	3	41	theme	significant	442:452	arg1	co-receptor					464:474	a co-receptor	462:474	a co-receptor	462:474	In addition to ACE2, heparan sulfate (HS) on the surface of host cells also plays a significant role as a co-receptor.
34940684	2	42	theme	respiratory	176:186	arg1	coronavirus					197:207	severe acute respiratory syndrome coronavirus 2	163:209	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	163:222	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	2	42	theme	respiratory	176:186	arg1	SARS-CoV-2					212:221	SARS-CoV-2	212:221	SARS-CoV-2	212:221	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	2	42	theme	respiratory	176:186	arg1	pathogen					128:135	The pathogen	124:135	The pathogen responsible for COVID-19	124:160	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	1	43	theme	human	102:106	arg1	pandemic					82:89	The COVID-19 pandemic	69:89	The COVID-19 pandemic	69:89	The COVID-19 pandemic is a major human health concern.
34940684	1	43	theme	human	102:106	arg1	concern					115:121	a major human health concern	94:121	a major human health concern	94:121	The COVID-19 pandemic is a major human health concern.
34940684	6	44	theme	magnetic	969:976	arg1	resonance					978:986	two-dimensional nuclear magnetic resonance	945:986	two-dimensional nuclear magnetic resonance	945:986	The structural characteristics of RS were investigated by determining its monosaccharide composition and performing two-dimensional nuclear magnetic resonance.
34940684	3	45	theme	host	418:421	arg1	cells					423:427	host cells	418:427	host cells	418:427	In addition to ACE2, heparan sulfate (HS) on the surface of host cells also plays a significant role as a co-receptor.
34940684	2	46	theme	acute	170:174	arg1	coronavirus					197:207	severe acute respiratory syndrome coronavirus 2	163:209	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	163:222	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	2	46	theme	acute	170:174	arg1	SARS-CoV-2					212:221	SARS-CoV-2	212:221	SARS-CoV-2	212:221	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	2	46	theme	acute	170:174	arg1	pathogen					128:135	The pathogen	124:135	The pathogen responsible for COVID-19	124:160	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	3	47	dep	ACE2	373:376	arg1	addition					361:368	addition	361:368	addition	361:368	In addition to ACE2, heparan sulfate (HS) on the surface of host cells also plays a significant role as a co-receptor.
34940684	6	48	theme	nuclear	961:967	arg1	resonance					978:986	two-dimensional nuclear magnetic resonance	945:986	two-dimensional nuclear magnetic resonance	945:986	The structural characteristics of RS were investigated by determining its monosaccharide composition and performing two-dimensional nuclear magnetic resonance.
34940684	0	49	theme	Sulfate	36:42	arg1	Activity					16:23	Anti-SARS-CoV-2 Activity	0:23	Anti-SARS-CoV-2 Activity of Rhamnan Sulfate from Monostroma nitidum.	0:67	Anti-SARS-CoV-2 Activity of Rhamnan Sulfate from Monostroma nitidum.
34940684	6	50	theme	structural	833:842	arg1	characteristics					844:858	The structural characteristics	829:858	The structural characteristics of RS	829:864	The structural characteristics of RS were investigated by determining its monosaccharide composition and performing two-dimensional nuclear magnetic resonance.
34940684	7	51	theme	RS	989:990	arg1	inhibition					992:1001	RS inhibition	989:1001	RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants)	989:1135	RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants) was studied using surface plasmon resonance (SPR).
34940684	2	52	theme	severe	163:168	arg1	coronavirus					197:207	severe acute respiratory syndrome coronavirus 2	163:209	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	163:222	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	2	52	theme	severe	163:168	arg1	SARS-CoV-2					212:221	SARS-CoV-2	212:221	SARS-CoV-2	212:221	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	2	52	theme	severe	163:168	arg1	pathogen					128:135	The pathogen	124:135	The pathogen responsible for COVID-19	124:160	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	5	53	theme	viral	801:805	arg1	infectivity					807:817	viral infectivity	801:817	viral infectivity	801:817	In the current study, rhamnan sulfate (RS), a polysaccharide with a rhamnose backbone from a green seaweed, Monostroma nitidum, was evaluated for binding to the S-protein from SARS-CoV-2 and inhibition of viral infectivity in vitro.
34940684	6	54	theme	two-dimensional	945:959	arg1	resonance					978:986	two-dimensional nuclear magnetic resonance	945:986	two-dimensional nuclear magnetic resonance	945:986	The structural characteristics of RS were investigated by determining its monosaccharide composition and performing two-dimensional nuclear magnetic resonance.
34940684	8	55	theme	S-protein	1247:1255	arg1	RBD					1282:1284	RBD	1282:1284	RBD	1282:1284	In competitive binding studies, the IC50 of RS against the S-protein receptor binding domain (RBD) binding to immobilized heparin was 1.6 ng/mL, which is much lower than the IC50 for heparin (~750 ng/mL).
34940684	8	55	theme	S-protein	1247:1255	arg1	domain					1274:1279	the S-protein receptor binding domain	1243:1279	the S-protein receptor binding domain (RBD) binding to immobilized heparin	1243:1316	In competitive binding studies, the IC50 of RS against the S-protein receptor binding domain (RBD) binding to immobilized heparin was 1.6 ng/mL, which is much lower than the IC50 for heparin (~750 ng/mL).
34940684	9	56	theme	S-protein	1443:1451	arg1	RBD					1453:1455	the S-protein RBD or pseudoviral particles	1439:1480	RBD	1453:1455	RS showed stronger inhibition than heparin on the S-protein RBD or pseudoviral particles binding to immobilized heparin.
34940684	5	57	from	seaweed	695:701	arg1	backbone					673:680	a rhamnose backbone	662:680	a rhamnose backbone from a green seaweed, Monostroma nitidum	662:721	In the current study, rhamnan sulfate (RS), a polysaccharide with a rhamnose backbone from a green seaweed, Monostroma nitidum, was evaluated for binding to the S-protein from SARS-CoV-2 and inhibition of viral infectivity in vitro.
34940684	1	58	theme	health	108:113	arg1	pandemic					82:89	The COVID-19 pandemic	69:89	The COVID-19 pandemic	69:89	The COVID-19 pandemic is a major human health concern.
34940684	1	58	theme	health	108:113	arg1	concern					115:121	a major human health concern	94:121	a major human health concern	94:121	The COVID-19 pandemic is a major human health concern.
34940684	0	59	theme	Monostroma	49:58	arg1	nitidum					60:66	Monostroma nitidum	49:66	Monostroma nitidum	49:66	Anti-SARS-CoV-2 Activity of Rhamnan Sulfate from Monostroma nitidum.
34940684	7	60	theme	wild	1096:1099	arg1	type					1101:1104	wild type	1096:1104	wild type	1096:1104	RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants) was studied using surface plasmon resonance (SPR).
34940684	10	61	theme	delta	1631:1635	arg1	variant					1637:1643	the delta variant	1627:1643	the delta variant	1627:1643	Finally, in an in vitro cell-based assay, RS showed strong antiviral activities against wild type SARS-CoV-2 and the delta variant.
34940684	2	62	theme	host	298:301	arg1	receptor					308:315	a host cell receptor	296:315	a host cell receptor	296:315	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	2	62	theme	host	298:301	arg1	enzyme					341:346	angiotensin-converting enzyme 2	318:348	angiotensin-converting enzyme 2 (ACE2)	318:355	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	7	63	theme	SARS-CoV-2	1065:1074	arg1	protein					1082:1088	the SARS-CoV-2 spike protein	1061:1088	the SARS-CoV-2 spike protein (from wild type and different mutant variants)	1061:1135	RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants) was studied using surface plasmon resonance (SPR).
34940684	5	64	theme	current	603:609	arg1	study					611:615	the current study	599:615	the current study	599:615	In the current study, rhamnan sulfate (RS), a polysaccharide with a rhamnose backbone from a green seaweed, Monostroma nitidum, was evaluated for binding to the S-protein from SARS-CoV-2 and inhibition of viral infectivity in vitro.
34940684	10	65	theme	wild	1602:1605	arg1	type					1607:1610	wild type SARS-CoV-2 and the delta variant	1602:1643	type	1607:1610	Finally, in an in vitro cell-based assay, RS showed strong antiviral activities against wild type SARS-CoV-2 and the delta variant.
34940684	5	66	theme	infectivity	807:817	arg1	inhibition					787:796	inhibition	787:796	inhibition of viral infectivity	787:817	In the current study, rhamnan sulfate (RS), a polysaccharide with a rhamnose backbone from a green seaweed, Monostroma nitidum, was evaluated for binding to the S-protein from SARS-CoV-2 and inhibition of viral infectivity in vitro.
34940684	5	66	theme	infectivity	807:817	arg1	binding					742:748	binding	742:748	binding to the S-protein from SARS-CoV-2	742:781	In the current study, rhamnan sulfate (RS), a polysaccharide with a rhamnose backbone from a green seaweed, Monostroma nitidum, was evaluated for binding to the S-protein from SARS-CoV-2 and inhibition of viral infectivity in vitro.
34940684	8	67	theme	binding	1266:1272	arg1	RBD					1282:1284	RBD	1282:1284	RBD	1282:1284	In competitive binding studies, the IC50 of RS against the S-protein receptor binding domain (RBD) binding to immobilized heparin was 1.6 ng/mL, which is much lower than the IC50 for heparin (~750 ng/mL).
34940684	8	67	theme	binding	1266:1272	arg1	domain					1274:1279	the S-protein receptor binding domain	1243:1279	the S-protein receptor binding domain (RBD) binding to immobilized heparin	1243:1316	In competitive binding studies, the IC50 of RS against the S-protein receptor binding domain (RBD) binding to immobilized heparin was 1.6 ng/mL, which is much lower than the IC50 for heparin (~750 ng/mL).
34940684	10	68	theme	antiviral	1573:1581	arg1	activities					1583:1592	strong antiviral activities	1566:1592	strong antiviral activities against wild type SARS-CoV-2 and the delta variant	1566:1643	Finally, in an in vitro cell-based assay, RS showed strong antiviral activities against wild type SARS-CoV-2 and the delta variant.
34940684	7	69	from	variants	1127:1134	arg1	protein					1082:1088	the SARS-CoV-2 spike protein	1061:1088	the SARS-CoV-2 spike protein (from wild type and different mutant variants)	1061:1135	RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants) was studied using surface plasmon resonance (SPR).
34940684	9	70	theme	pseudoviral	1460:1470	arg1	particles					1472:1480	the S-protein RBD or pseudoviral particles	1439:1480	particles	1472:1480	RS showed stronger inhibition than heparin on the S-protein RBD or pseudoviral particles binding to immobilized heparin.
34940684	7	71	theme	surface	1155:1161	arg1	resonance					1171:1179	surface plasmon resonance	1155:1179	surface plasmon resonance (SPR)	1155:1185	RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants) was studied using surface plasmon resonance (SPR).
34940684	7	71	theme	surface	1155:1161	arg1	SPR					1182:1184	SPR	1182:1184	SPR	1182:1184	RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants) was studied using surface plasmon resonance (SPR).
34940684	2	72	with	interaction	254:264	arg1	receptor					308:315	a host cell receptor	296:315	a host cell receptor	296:315	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	2	72	with	interaction	254:264	arg1	enzyme					341:346	angiotensin-converting enzyme 2	318:348	angiotensin-converting enzyme 2 (ACE2)	318:355	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	2	73	theme	syndrome	188:195	arg1	coronavirus					197:207	severe acute respiratory syndrome coronavirus 2	163:209	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	163:222	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	2	73	theme	syndrome	188:195	arg1	SARS-CoV-2					212:221	SARS-CoV-2	212:221	SARS-CoV-2	212:221	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	2	73	theme	syndrome	188:195	arg1	pathogen					128:135	The pathogen	124:135	The pathogen responsible for COVID-19	124:160	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	5	74	with	polysaccharide	642:655	arg1	backbone					673:680	a rhamnose backbone	662:680	a rhamnose backbone from a green seaweed, Monostroma nitidum	662:721	In the current study, rhamnan sulfate (RS), a polysaccharide with a rhamnose backbone from a green seaweed, Monostroma nitidum, was evaluated for binding to the S-protein from SARS-CoV-2 and inhibition of viral infectivity in vitro.
34940684	10	75	theme	strong	1566:1571	arg1	activities					1583:1592	strong antiviral activities	1566:1592	strong antiviral activities against wild type SARS-CoV-2 and the delta variant	1566:1643	Finally, in an in vitro cell-based assay, RS showed strong antiviral activities against wild type SARS-CoV-2 and the delta variant.
34940684	8	76	from	ng/mL	1326:1330	arg1	studies					1211:1217	competitive binding studies	1191:1217	competitive binding studies	1191:1217	In competitive binding studies, the IC50 of RS against the S-protein receptor binding domain (RBD) binding to immobilized heparin was 1.6 ng/mL, which is much lower than the IC50 for heparin (~750 ng/mL).
34940684	8	77	theme	immobilized	1298:1308	arg1	heparin					1310:1316	immobilized heparin	1298:1316	immobilized heparin	1298:1316	In competitive binding studies, the IC50 of RS against the S-protein receptor binding domain (RBD) binding to immobilized heparin was 1.6 ng/mL, which is much lower than the IC50 for heparin (~750 ng/mL).
34940684	2	78	theme	angiotensin-converting	318:339	arg1	ACE2					351:354	ACE2	351:354	ACE2	351:354	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	2	78	theme	angiotensin-converting	318:339	arg1	receptor					308:315	a host cell receptor	296:315	a host cell receptor	296:315	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	2	78	theme	angiotensin-converting	318:339	arg1	enzyme					341:346	angiotensin-converting enzyme 2	318:348	angiotensin-converting enzyme 2 (ACE2)	318:355	The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2).
34940684	6	79	theme	monosaccharide	903:916	arg1	composition					918:928	its monosaccharide composition	899:928	its monosaccharide composition	899:928	The structural characteristics of RS were investigated by determining its monosaccharide composition and performing two-dimensional nuclear magnetic resonance.
32933669	0	0	theme	chitosan/donor-acceptor	84:106	arg1	blends					116:121	chitosan/donor-acceptor polymer blends	84:121	chitosan/donor-acceptor polymer blends	84:121	Optical, morphological and photocatalytic properties of biobased tractable films of chitosan/donor-acceptor polymer blends.
32933669	3	1	theme	contact	708:714	arg1	analysis					722:729	contact angle analysis	708:729	contact angle analysis	708:729	The fabricated biobased films were characterized using spectroscopic techniques (FT-IR and UV-vis), thermogravimetry, mechanical assays, contact angle analysis, and atomic force microscopy (AFM).
32933669	1	2	with	chitosan	173:180	arg1	polymer					192:198	polymer	192:198	polymer bearing carbazole derivatives as pendant groups and fluorene-thiophene as donor-acceptor units (referred to as DA)	192:313	Biobased tractable films consisting of blends of chitosan (CS) with polymer bearing carbazole derivatives as pendant groups and fluorene-thiophene as donor-acceptor units (referred to as DA) were prepared, and their optical, morphological and photocatalytic properties were studied.
32933669	1	3	theme	chitosan	173:180	arg1	blends					163:168	blends	163:168	blends of chitosan (CS) with polymer bearing carbazole derivatives as pendant groups and fluorene-thiophene as donor-acceptor units (referred to as DA)	163:313	Biobased tractable films consisting of blends of chitosan (CS) with polymer bearing carbazole derivatives as pendant groups and fluorene-thiophene as donor-acceptor units (referred to as DA) were prepared, and their optical, morphological and photocatalytic properties were studied.
32933669	8	4	theme	synergetic	1656:1665	arg1	effects					1667:1673	synergetic effects	1656:1673	synergetic effects	1656:1673	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	2	5	theme	aqueous	477:483	arg1	solution					485:492	an acidified aqueous solution	464:492	an acidified aqueous solution containing chitosan to obtain chitosan/DA (CS/DA) films by solution casting	464:568	DA was dissolved in tetrahydrofuran (THF) and mixed with an acidified aqueous solution containing chitosan to obtain chitosan/DA (CS/DA) films by solution casting.
32933669	1	6	theme	optical	340:346	arg1	properties					382:391	their optical, morphological and photocatalytic properties	334:391	their optical, morphological and photocatalytic properties	334:391	Biobased tractable films consisting of blends of chitosan (CS) with polymer bearing carbazole derivatives as pendant groups and fluorene-thiophene as donor-acceptor units (referred to as DA) were prepared, and their optical, morphological and photocatalytic properties were studied.
32933669	6	7	theme	optical	1083:1089	arg1	topographical					1092:1104	the optical, topographical, and contact angle properties	1079:1134	topographical	1092:1104	This behavior was reflected in the optical, topographical, and contact angle properties of the films, which exhibited different characteristics before and after visible-light exposure.
32933669	1	8	theme	donor-acceptor	274:287	arg1	derivatives					218:228	carbazole derivatives	208:228	carbazole derivatives as pendant groups	208:246	Biobased tractable films consisting of blends of chitosan (CS) with polymer bearing carbazole derivatives as pendant groups and fluorene-thiophene as donor-acceptor units (referred to as DA) were prepared, and their optical, morphological and photocatalytic properties were studied.
32933669	1	8	theme	donor-acceptor	274:287	arg1	fluorene-thiophene					252:269	fluorene-thiophene	252:269	fluorene-thiophene	252:269	Biobased tractable films consisting of blends of chitosan (CS) with polymer bearing carbazole derivatives as pendant groups and fluorene-thiophene as donor-acceptor units (referred to as DA) were prepared, and their optical, morphological and photocatalytic properties were studied.
32933669	1	8	theme	donor-acceptor	274:287	arg1	units					289:293	donor-acceptor units	274:293	donor-acceptor units (referred to as DA)	274:313	Biobased tractable films consisting of blends of chitosan (CS) with polymer bearing carbazole derivatives as pendant groups and fluorene-thiophene as donor-acceptor units (referred to as DA) were prepared, and their optical, morphological and photocatalytic properties were studied.
32933669	0	9	theme	blends	116:121	arg1	films					75:79	biobased tractable films	56:79	biobased tractable films of chitosan/donor-acceptor polymer blends	56:121	Optical, morphological and photocatalytic properties of biobased tractable films of chitosan/donor-acceptor polymer blends.
32933669	4	10	theme	films	870:874	arg1	irradiation					851:861	visible-light irradiation	837:861	visible-light irradiation of the films	837:874	The effects of varying DA compositions and the results of exposure to visible-light irradiation of the films were also analyzed.
32933669	8	11	theme	films	1601:1605	arg1	photoproperties					1723:1737	the photoproperties	1719:1737	the photoproperties of DA	1719:1743	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	8	11	theme	films	1601:1605	arg1	activity					1429:1436	a significant photocatalytic activity	1400:1436	a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan	1400:1713	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	8	12	theme	photocatalytic	1414:1427	arg1	activity					1429:1436	a significant photocatalytic activity	1400:1436	a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan	1400:1713	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	0	13	theme	polymer	108:114	arg1	blends					116:121	chitosan/donor-acceptor polymer blends	84:121	chitosan/donor-acceptor polymer blends	84:121	Optical, morphological and photocatalytic properties of biobased tractable films of chitosan/donor-acceptor polymer blends.
32933669	1	14	theme	morphological	349:361	arg1	properties					382:391	their optical, morphological and photocatalytic properties	334:391	their optical, morphological and photocatalytic properties	334:391	Biobased tractable films consisting of blends of chitosan (CS) with polymer bearing carbazole derivatives as pendant groups and fluorene-thiophene as donor-acceptor units (referred to as DA) were prepared, and their optical, morphological and photocatalytic properties were studied.
32933669	8	15	theme	DA	1742:1743	arg1	photoproperties					1723:1737	the photoproperties	1719:1737	the photoproperties of DA	1719:1743	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	8	15	theme	DA	1742:1743	arg1	activity					1429:1436	a significant photocatalytic activity	1400:1436	a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan	1400:1713	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	7	16	theme	photocatalytic	1246:1259	arg1	performance					1261:1271	the photocatalytic performance	1242:1271	the photocatalytic performance of the biobased films	1242:1293	Finally, the photocatalytic performance of the biobased films was tested via the decomposition of methyl orange (MO), as a reaction model system.
32933669	7	16	theme	photocatalytic	1246:1259	arg1	system					1371:1376	a reaction model system	1354:1376	a reaction model system	1354:1376	Finally, the photocatalytic performance of the biobased films was tested via the decomposition of methyl orange (MO), as a reaction model system.
32933669	6	17	theme	different	1166:1174	arg1	characteristics					1176:1190	different characteristics	1166:1190	different characteristics	1166:1190	This behavior was reflected in the optical, topographical, and contact angle properties of the films, which exhibited different characteristics before and after visible-light exposure.
32933669	8	18	theme	ability	1695:1701	arg1	presence					1643:1650	combined presence	1634:1650	combined presence	1634:1650	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	8	18	theme	ability	1695:1701	arg1	effects					1667:1673	synergetic effects	1656:1673	synergetic effects	1656:1673	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	8	19	dep	activity	1429:1436	arg1	according					1439:1447	according	1439:1447	according to biobased film composition	1439:1476	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	7	20	theme	films	1289:1293	arg1	performance					1261:1271	the photocatalytic performance	1242:1271	the photocatalytic performance of the biobased films	1242:1293	Finally, the photocatalytic performance of the biobased films was tested via the decomposition of methyl orange (MO), as a reaction model system.
32933669	7	20	theme	films	1289:1293	arg1	system					1371:1376	a reaction model system	1354:1376	a reaction model system	1354:1376	Finally, the photocatalytic performance of the biobased films was tested via the decomposition of methyl orange (MO), as a reaction model system.
32933669	4	21	theme	exposure	825:832	arg1	effects					771:777	The effects	767:777	The effects of varying DA compositions	767:804	The effects of varying DA compositions and the results of exposure to visible-light irradiation of the films were also analyzed.
32933669	4	21	theme	exposure	825:832	arg1	results					814:820	the results	810:820	the results of exposure to visible-light irradiation of the films	810:874	The effects of varying DA compositions and the results of exposure to visible-light irradiation of the films were also analyzed.
32933669	8	22	theme	film-forming	1682:1693	arg1	ability					1695:1701	the film-forming ability	1678:1701	the film-forming ability of chitosan	1678:1713	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	7	23	theme	orange	1338:1343	arg1	decomposition					1314:1326	the decomposition	1310:1326	the decomposition of methyl orange (MO)	1310:1348	Finally, the photocatalytic performance of the biobased films was tested via the decomposition of methyl orange (MO), as a reaction model system.
32933669	1	24	theme	photocatalytic	367:380	arg1	properties					382:391	their optical, morphological and photocatalytic properties	334:391	their optical, morphological and photocatalytic properties	334:391	Biobased tractable films consisting of blends of chitosan (CS) with polymer bearing carbazole derivatives as pendant groups and fluorene-thiophene as donor-acceptor units (referred to as DA) were prepared, and their optical, morphological and photocatalytic properties were studied.
32933669	8	25	theme	film	1461:1464	arg1	composition					1466:1476	biobased film composition	1452:1476	biobased film composition	1452:1476	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	8	26	theme	methyl	1510:1515	arg1	orange					1517:1522	methyl orange	1510:1522	methyl orange	1510:1522	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	4	27	theme	visible-light	837:849	arg1	irradiation					851:861	visible-light irradiation	837:861	visible-light irradiation of the films	837:874	The effects of varying DA compositions and the results of exposure to visible-light irradiation of the films were also analyzed.
32933669	0	28	theme	Optical	0:6	arg1	properties					42:51	Optical, morphological and photocatalytic properties	0:51	Optical, morphological and photocatalytic properties of biobased tractable films of chitosan/donor-acceptor polymer blends.	0:122	Optical, morphological and photocatalytic properties of biobased tractable films of chitosan/donor-acceptor polymer blends.
32933669	5	29	theme	films	1041:1045	arg1	existence					922:930	the existence	918:930	the existence of interactions between chitosan and DA	918:970	The results indicated the existence of interactions between chitosan and DA and a potentially profitable light-driven response of these biobased films.
32933669	5	29	theme	films	1041:1045	arg1	response					1014:1021	a potentially profitable light-driven response	976:1021	a potentially profitable light-driven response of these biobased films	976:1045	The results indicated the existence of interactions between chitosan and DA and a potentially profitable light-driven response of these biobased films.
32933669	7	30	theme	methyl	1331:1336	arg1	MO					1346:1347	MO	1346:1347	MO	1346:1347	Finally, the photocatalytic performance of the biobased films was tested via the decomposition of methyl orange (MO), as a reaction model system.
32933669	7	30	theme	methyl	1331:1336	arg1	orange					1338:1343	methyl orange	1331:1343	methyl orange (MO)	1331:1348	Finally, the photocatalytic performance of the biobased films was tested via the decomposition of methyl orange (MO), as a reaction model system.
32933669	5	31	theme	profitable	990:999	arg1	response					1014:1021	a potentially profitable light-driven response	976:1021	a potentially profitable light-driven response of these biobased films	976:1045	The results indicated the existence of interactions between chitosan and DA and a potentially profitable light-driven response of these biobased films.
32933669	5	32	theme	biobased	1032:1039	arg1	films					1041:1045	these biobased films	1026:1045	these biobased films	1026:1045	The results indicated the existence of interactions between chitosan and DA and a potentially profitable light-driven response of these biobased films.
32933669	5	33	theme	interactions	935:946	arg1	existence					922:930	the existence	918:930	the existence of interactions between chitosan and DA	918:970	The results indicated the existence of interactions between chitosan and DA and a potentially profitable light-driven response of these biobased films.
32933669	5	33	theme	interactions	935:946	arg1	response					1014:1021	a potentially profitable light-driven response	976:1021	a potentially profitable light-driven response of these biobased films	976:1045	The results indicated the existence of interactions between chitosan and DA and a potentially profitable light-driven response of these biobased films.
32933669	2	34	theme	tetrahydrofuran	427:441	arg1	THF					444:446	tetrahydrofuran (THF)	427:447	tetrahydrofuran (THF)	427:447	DA was dissolved in tetrahydrofuran (THF) and mixed with an acidified aqueous solution containing chitosan to obtain chitosan/DA (CS/DA) films by solution casting.
32933669	0	35	theme	morphological	9:21	arg1	properties					42:51	Optical, morphological and photocatalytic properties	0:51	Optical, morphological and photocatalytic properties of biobased tractable films of chitosan/donor-acceptor polymer blends.	0:122	Optical, morphological and photocatalytic properties of biobased tractable films of chitosan/donor-acceptor polymer blends.
32933669	3	36	theme	atomic	736:741	arg1	microscopy					749:758	atomic force microscopy	736:758	atomic force microscopy (AFM)	736:764	The fabricated biobased films were characterized using spectroscopic techniques (FT-IR and UV-vis), thermogravimetry, mechanical assays, contact angle analysis, and atomic force microscopy (AFM).
32933669	3	36	theme	atomic	736:741	arg1	AFM					761:763	AFM	761:763	AFM	761:763	The fabricated biobased films were characterized using spectroscopic techniques (FT-IR and UV-vis), thermogravimetry, mechanical assays, contact angle analysis, and atomic force microscopy (AFM).
32933669	8	37	theme	combined	1634:1641	arg1	presence					1643:1650	combined presence	1634:1650	combined presence	1634:1650	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	1	38	theme	Biobased	124:131	arg1	films					143:147	Biobased tractable films	124:147	Biobased tractable films consisting of blends of chitosan (CS) with polymer bearing carbazole derivatives as pendant groups and fluorene-thiophene as donor-acceptor units (referred to as DA)	124:313	Biobased tractable films consisting of blends of chitosan (CS) with polymer bearing carbazole derivatives as pendant groups and fluorene-thiophene as donor-acceptor units (referred to as DA) were prepared, and their optical, morphological and photocatalytic properties were studied.
32933669	8	39	theme	continuous	1544:1553	arg1	irradiation					1569:1579	continuous visible-light irradiation	1544:1579	continuous visible-light irradiation for 120 min	1544:1591	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	1	40	theme	carbazole	208:216	arg1	units					289:293	donor-acceptor units	274:293	donor-acceptor units (referred to as DA)	274:313	Biobased tractable films consisting of blends of chitosan (CS) with polymer bearing carbazole derivatives as pendant groups and fluorene-thiophene as donor-acceptor units (referred to as DA) were prepared, and their optical, morphological and photocatalytic properties were studied.
32933669	1	40	theme	carbazole	208:216	arg1	fluorene-thiophene					252:269	fluorene-thiophene	252:269	fluorene-thiophene	252:269	Biobased tractable films consisting of blends of chitosan (CS) with polymer bearing carbazole derivatives as pendant groups and fluorene-thiophene as donor-acceptor units (referred to as DA) were prepared, and their optical, morphological and photocatalytic properties were studied.
32933669	1	40	theme	carbazole	208:216	arg1	derivatives					218:228	carbazole derivatives	208:228	carbazole derivatives as pendant groups	208:246	Biobased tractable films consisting of blends of chitosan (CS) with polymer bearing carbazole derivatives as pendant groups and fluorene-thiophene as donor-acceptor units (referred to as DA) were prepared, and their optical, morphological and photocatalytic properties were studied.
32933669	2	41	theme	acidified	467:475	arg1	solution					485:492	an acidified aqueous solution	464:492	an acidified aqueous solution containing chitosan to obtain chitosan/DA (CS/DA) films by solution casting	464:568	DA was dissolved in tetrahydrofuran (THF) and mixed with an acidified aqueous solution containing chitosan to obtain chitosan/DA (CS/DA) films by solution casting.
32933669	3	42	theme	force	743:747	arg1	microscopy					749:758	atomic force microscopy	736:758	atomic force microscopy (AFM)	736:764	The fabricated biobased films were characterized using spectroscopic techniques (FT-IR and UV-vis), thermogravimetry, mechanical assays, contact angle analysis, and atomic force microscopy (AFM).
32933669	3	42	theme	force	743:747	arg1	AFM					761:763	AFM	761:763	AFM	761:763	The fabricated biobased films were characterized using spectroscopic techniques (FT-IR and UV-vis), thermogravimetry, mechanical assays, contact angle analysis, and atomic force microscopy (AFM).
32933669	7	43	theme	model	1365:1369	arg1	performance					1261:1271	the photocatalytic performance	1242:1271	the photocatalytic performance of the biobased films	1242:1293	Finally, the photocatalytic performance of the biobased films was tested via the decomposition of methyl orange (MO), as a reaction model system.
32933669	7	43	theme	model	1365:1369	arg1	system					1371:1376	a reaction model system	1354:1376	a reaction model system	1354:1376	Finally, the photocatalytic performance of the biobased films was tested via the decomposition of methyl orange (MO), as a reaction model system.
32933669	0	44	theme	photocatalytic	27:40	arg1	properties					42:51	Optical, morphological and photocatalytic properties	0:51	Optical, morphological and photocatalytic properties of biobased tractable films of chitosan/donor-acceptor polymer blends.	0:122	Optical, morphological and photocatalytic properties of biobased tractable films of chitosan/donor-acceptor polymer blends.
32933669	2	45	theme	CS/DA	537:541	arg1	films					544:548	chitosan/DA (CS/DA) films	524:548	chitosan/DA (CS/DA) films	524:548	DA was dissolved in tetrahydrofuran (THF) and mixed with an acidified aqueous solution containing chitosan to obtain chitosan/DA (CS/DA) films by solution casting.
32933669	8	46	theme	biobased	1452:1459	arg1	composition					1466:1476	biobased film composition	1452:1476	biobased film composition	1452:1476	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	4	47	theme	compositions	793:804	arg1	effects					771:777	The effects	767:777	The effects of varying DA compositions	767:804	The effects of varying DA compositions and the results of exposure to visible-light irradiation of the films were also analyzed.
32933669	4	47	theme	compositions	793:804	arg1	results					814:820	the results	810:820	the results of exposure to visible-light irradiation of the films	810:874	The effects of varying DA compositions and the results of exposure to visible-light irradiation of the films were also analyzed.
32933669	7	48	theme	reaction	1356:1363	arg1	performance					1261:1271	the photocatalytic performance	1242:1271	the photocatalytic performance of the biobased films	1242:1293	Finally, the photocatalytic performance of the biobased films was tested via the decomposition of methyl orange (MO), as a reaction model system.
32933669	7	48	theme	reaction	1356:1363	arg1	system					1371:1376	a reaction model system	1354:1376	a reaction model system	1354:1376	Finally, the photocatalytic performance of the biobased films was tested via the decomposition of methyl orange (MO), as a reaction model system.
32933669	3	49	dep	techniques	640:649	arg1	UV-vis					662:667	UV-vis	662:667	UV-vis	662:667	The fabricated biobased films were characterized using spectroscopic techniques (FT-IR and UV-vis), thermogravimetry, mechanical assays, contact angle analysis, and atomic force microscopy (AFM).
32933669	3	49	dep	techniques	640:649	arg1	FT-IR					652:656	FT-IR	652:656	FT-IR	652:656	The fabricated biobased films were characterized using spectroscopic techniques (FT-IR and UV-vis), thermogravimetry, mechanical assays, contact angle analysis, and atomic force microscopy (AFM).
32933669	0	50	theme	biobased	56:63	arg1	films					75:79	biobased tractable films	56:79	biobased tractable films of chitosan/donor-acceptor polymer blends	56:121	Optical, morphological and photocatalytic properties of biobased tractable films of chitosan/donor-acceptor polymer blends.
32933669	8	51	theme	visible-light	1555:1567	arg1	irradiation					1569:1579	continuous visible-light irradiation	1544:1579	continuous visible-light irradiation for 120 min	1544:1591	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	3	52	theme	fabricated	575:584	arg1	films					595:599	The fabricated biobased films	571:599	The fabricated biobased films	571:599	The fabricated biobased films were characterized using spectroscopic techniques (FT-IR and UV-vis), thermogravimetry, mechanical assays, contact angle analysis, and atomic force microscopy (AFM).
32933669	3	53	theme	angle	716:720	arg1	analysis					722:729	contact angle analysis	708:729	contact angle analysis	708:729	The fabricated biobased films were characterized using spectroscopic techniques (FT-IR and UV-vis), thermogravimetry, mechanical assays, contact angle analysis, and atomic force microscopy (AFM).
32933669	1	54	theme	tractable	133:141	arg1	films					143:147	Biobased tractable films	124:147	Biobased tractable films consisting of blends of chitosan (CS) with polymer bearing carbazole derivatives as pendant groups and fluorene-thiophene as donor-acceptor units (referred to as DA)	124:313	Biobased tractable films consisting of blends of chitosan (CS) with polymer bearing carbazole derivatives as pendant groups and fluorene-thiophene as donor-acceptor units (referred to as DA) were prepared, and their optical, morphological and photocatalytic properties were studied.
32933669	8	55	theme	orange	1517:1522	arg1	%					1496:1496	approximately 64 %	1479:1496	approximately 64 %	1479:1496	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	8	55	theme	orange	1517:1522	arg1	%					1505:1505	87 %	1502:1505	87 % of methyl orange	1502:1522	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	8	55	theme	orange	1517:1522	arg1	orange					1517:1522	methyl orange	1510:1522	methyl orange	1510:1522	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	1	56	dep	units	289:293	arg1	referred					296:303	referred	296:303	referred to as DA	296:312	Biobased tractable films consisting of blends of chitosan (CS) with polymer bearing carbazole derivatives as pendant groups and fluorene-thiophene as donor-acceptor units (referred to as DA) were prepared, and their optical, morphological and photocatalytic properties were studied.
32933669	3	57	theme	biobased	586:593	arg1	films					595:599	The fabricated biobased films	571:599	The fabricated biobased films	571:599	The fabricated biobased films were characterized using spectroscopic techniques (FT-IR and UV-vis), thermogravimetry, mechanical assays, contact angle analysis, and atomic force microscopy (AFM).
32933669	2	58	contain	containing	494:503	arg2	chitosan					505:512	chitosan	505:512	chitosan to obtain chitosan/DA (CS/DA) films by solution casting	505:568	DA was dissolved in tetrahydrofuran (THF) and mixed with an acidified aqueous solution containing chitosan to obtain chitosan/DA (CS/DA) films by solution casting.
32933669	2	58	contain	containing	494:503	arg1	solution					485:492	an acidified aqueous solution	464:492	an acidified aqueous solution containing chitosan to obtain chitosan/DA (CS/DA) films by solution casting	464:568	DA was dissolved in tetrahydrofuran (THF) and mixed with an acidified aqueous solution containing chitosan to obtain chitosan/DA (CS/DA) films by solution casting.
32933669	6	59	theme	angle	1119:1123	arg1	properties					1125:1134	the optical, topographical, and contact angle properties	1079:1134	properties	1125:1134	This behavior was reflected in the optical, topographical, and contact angle properties of the films, which exhibited different characteristics before and after visible-light exposure.
32933669	8	60	theme	significant	1402:1412	arg1	activity					1429:1436	a significant photocatalytic activity	1400:1436	a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan	1400:1713	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	5	61	theme	light-driven	1001:1012	arg1	response					1014:1021	a potentially profitable light-driven response	976:1021	a potentially profitable light-driven response of these biobased films	976:1045	The results indicated the existence of interactions between chitosan and DA and a potentially profitable light-driven response of these biobased films.
32933669	3	62	theme	mechanical	689:698	arg1	assays					700:705	mechanical assays	689:705	mechanical assays	689:705	The fabricated biobased films were characterized using spectroscopic techniques (FT-IR and UV-vis), thermogravimetry, mechanical assays, contact angle analysis, and atomic force microscopy (AFM).
32933669	6	63	theme	visible-light	1209:1221	arg1	exposure					1223:1230	visible-light exposure	1209:1230	visible-light exposure	1209:1230	This behavior was reflected in the optical, topographical, and contact angle properties of the films, which exhibited different characteristics before and after visible-light exposure.
32933669	3	64	theme	spectroscopic	626:638	arg1	techniques					640:649	spectroscopic techniques	626:649	spectroscopic techniques (FT-IR and UV-vis)	626:668	The fabricated biobased films were characterized using spectroscopic techniques (FT-IR and UV-vis), thermogravimetry, mechanical assays, contact angle analysis, and atomic force microscopy (AFM).
32933669	6	65	theme	contact	1111:1117	arg1	properties					1125:1134	the optical, topographical, and contact angle properties	1079:1134	properties	1125:1134	This behavior was reflected in the optical, topographical, and contact angle properties of the films, which exhibited different characteristics before and after visible-light exposure.
32933669	1	66	theme	pendant	233:239	arg1	groups					241:246	pendant groups	233:246	pendant groups	233:246	Biobased tractable films consisting of blends of chitosan (CS) with polymer bearing carbazole derivatives as pendant groups and fluorene-thiophene as donor-acceptor units (referred to as DA) were prepared, and their optical, morphological and photocatalytic properties were studied.
32933669	2	67	theme	solution	553:560	arg1	casting					562:568	solution casting	553:568	solution casting	553:568	DA was dissolved in tetrahydrofuran (THF) and mixed with an acidified aqueous solution containing chitosan to obtain chitosan/DA (CS/DA) films by solution casting.
32933669	7	68	theme	biobased	1280:1287	arg1	films					1289:1293	the biobased films	1276:1293	the biobased films	1276:1293	Finally, the photocatalytic performance of the biobased films was tested via the decomposition of methyl orange (MO), as a reaction model system.
32933669	8	69	theme	chitosan	1706:1713	arg1	ability					1695:1701	the film-forming ability	1678:1701	the film-forming ability of chitosan	1678:1713	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	0	70	theme	films	75:79	arg1	properties					42:51	Optical, morphological and photocatalytic properties	0:51	Optical, morphological and photocatalytic properties of biobased tractable films of chitosan/donor-acceptor polymer blends.	0:122	Optical, morphological and photocatalytic properties of biobased tractable films of chitosan/donor-acceptor polymer blends.
32933669	4	71	theme	DA	790:791	arg1	compositions					793:804	varying DA compositions	782:804	varying DA compositions	782:804	The effects of varying DA compositions and the results of exposure to visible-light irradiation of the films were also analyzed.
32933669	8	72	dep	presence	1643:1650	arg1	the					1630:1632	the	1630:1632	the	1630:1632	Our results revealed a significant photocatalytic activity (according to biobased film composition, approximately 64 % and 87 % of methyl orange were degraded under continuous visible-light irradiation for 120 min) of the films which is attributed to the combined presence and synergetic effects of the film-forming ability of chitosan and the photoproperties of DA.
32933669	6	73	theme	films	1143:1147	arg1	topographical					1092:1104	the optical, topographical, and contact angle properties	1079:1134	topographical	1092:1104	This behavior was reflected in the optical, topographical, and contact angle properties of the films, which exhibited different characteristics before and after visible-light exposure.
32933669	6	73	theme	films	1143:1147	arg1	properties					1125:1134	the optical, topographical, and contact angle properties	1079:1134	properties	1125:1134	This behavior was reflected in the optical, topographical, and contact angle properties of the films, which exhibited different characteristics before and after visible-light exposure.
32933669	0	74	theme	tractable	65:73	arg1	films					75:79	biobased tractable films	56:79	biobased tractable films of chitosan/donor-acceptor polymer blends	56:121	Optical, morphological and photocatalytic properties of biobased tractable films of chitosan/donor-acceptor polymer blends.
32933669	4	75	theme	varying	782:788	arg1	compositions					793:804	varying DA compositions	782:804	varying DA compositions	782:804	The effects of varying DA compositions and the results of exposure to visible-light irradiation of the films were also analyzed.
32933669	2	76	theme	chitosan/DA	524:534	arg1	films					544:548	chitosan/DA (CS/DA) films	524:548	chitosan/DA (CS/DA) films	524:548	DA was dissolved in tetrahydrofuran (THF) and mixed with an acidified aqueous solution containing chitosan to obtain chitosan/DA (CS/DA) films by solution casting.
33254970	6	0	theme	modulus	1117:1123	arg1	~35 MPa					1125:1131	compressive modulus ~35 MPa	1105:1131	compressive modulus ~35 MPa	1105:1131	The coated sponge showed a superior mechanical strength (compressive strength ~3.8 MPa, compressive modulus ~35 MPa) with appreciable flexibility and recoverability under repeated compressive loading-unloading cycles.
33254970	6	0	theme	modulus	1117:1123	arg1	~3.8 MPa					1095:1102	compressive strength ~3.8 MPa	1074:1102	compressive strength ~3.8 MPa	1074:1102	The coated sponge showed a superior mechanical strength (compressive strength ~3.8 MPa, compressive modulus ~35 MPa) with appreciable flexibility and recoverability under repeated compressive loading-unloading cycles.
33254970	2	1	theme	SA	364:365	arg1	CNFs					393:396	CNFs	393:396	CNFs	393:396	The composite bio-sponges were developed from the aqueous amalgamation of SA and cellulose nanofibres (CNFs) in bio-extracts like Rice water (Rw) and Giloy extract (Ge).
33254970	2	1	theme	SA	364:365	arg1	nanofibres					381:390	SA and cellulose nanofibres	364:390	nanofibres	381:390	The composite bio-sponges were developed from the aqueous amalgamation of SA and cellulose nanofibres (CNFs) in bio-extracts like Rice water (Rw) and Giloy extract (Ge).
33254970	8	2	theme	chains	1444:1449	arg1	entanglement					1422:1433	entanglement	1422:1433	entanglement	1422:1433	The physical entanglement of BioPU chains with core structural components of sponge improved their structural stability by suppressing their full fragmentation in water-based medium without affecting its swelling behaviour (swelling ratio > 1000%).
33254970	7	3	theme	coating	1297:1303	arg1	concentrations					1305:1318	BioPU coating concentrations	1291:1318	BioPU coating concentrations owing to the different degree of polymer chain entanglement within the sponge construct	1291:1406	A tunable degradation behaviour was achieved by varying BioPU coating concentrations owing to the different degree of polymer chain entanglement within the sponge construct.
33254970	1	4	theme	care	275:278	arg1	scaffold					280:287	wound care scaffold	269:287	wound care scaffold	269:287	In this work, sodium alginate (SA) based "all-natural" composite bio-sponges were designed for potential application as wound care scaffold.
33254970	2	5	from	amalgamation	348:359	arg1	bio-extracts					402:413	bio-extracts	402:413	bio-extracts like Rice water (Rw) and Giloy extract (Ge)	402:457	The composite bio-sponges were developed from the aqueous amalgamation of SA and cellulose nanofibres (CNFs) in bio-extracts like Rice water (Rw) and Giloy extract (Ge).
33254970	3	6	theme	care	688:691	arg1	scaffold					693:700	a wound care scaffold	680:700	a wound care scaffold	680:700	These sponges were modified by employing a simple coating strategy using vegetable oil-based bio-polyurethane (BioPU) to tailor their physicochemical and biological properties so as to match the specific requirements of a wound care scaffold.
33254970	3	7	theme	simple	503:508	arg1	strategy					518:525	a simple coating strategy	501:525	a simple coating strategy using vegetable oil-based bio-polyurethane (BioPU) to tailor their physicochemical and biological properties so as to match the specific requirements of a wound care scaffold	501:700	These sponges were modified by employing a simple coating strategy using vegetable oil-based bio-polyurethane (BioPU) to tailor their physicochemical and biological properties so as to match the specific requirements of a wound care scaffold.
33254970	5	8	theme	sponge	875:880	arg1	construct					882:890	the sponge construct	871:890	the sponge construct	871:890	The interpenetration of BioPU chains within the sponge construct resulted in the formation of numerous micro-networks in the interconnected microporous structure of sponges (porosity ≥75%).
33254970	9	9	theme	cells	1787:1791	arg1	growth					1772:1777	growth	1772:1777	growth	1772:1777	The coated sponge surface has provided a suitable moist-adherent physical environment to support the adhesion and growth of skin cells (HaCaT cells).
33254970	9	9	theme	cells	1787:1791	arg1	adhesion					1759:1766	adhesion	1759:1766	adhesion	1759:1766	The coated sponge surface has provided a suitable moist-adherent physical environment to support the adhesion and growth of skin cells (HaCaT cells).
33254970	6	10	theme	compressive	1197:1207	arg1	cycles					1227:1232	repeated compressive loading-unloading cycles	1188:1232	repeated compressive loading-unloading cycles	1188:1232	The coated sponge showed a superior mechanical strength (compressive strength ~3.8 MPa, compressive modulus ~35 MPa) with appreciable flexibility and recoverability under repeated compressive loading-unloading cycles.
33254970	11	11	dep	in	2047:2048	arg1	vivo					2050:2053	vivo	2050:2053	vivo	2050:2053	Moreover, no signs of skin erythema or edema were observed during in vivo dermal irritation and corrosion test performed on the skin of Sprague Dawley (SD) rats.
33254970	9	12	theme	HaCaT	1794:1798	arg1	cells					1787:1791	skin cells	1782:1791	skin cells (HaCaT cells)	1782:1805	The coated sponge surface has provided a suitable moist-adherent physical environment to support the adhesion and growth of skin cells (HaCaT cells).
33254970	9	12	theme	HaCaT	1794:1798	arg1	cells					1800:1804	HaCaT cells	1794:1804	HaCaT cells	1794:1804	The coated sponge surface has provided a suitable moist-adherent physical environment to support the adhesion and growth of skin cells (HaCaT cells).
33254970	9	13	theme	sponge	1669:1674	arg1	surface					1676:1682	The coated sponge surface	1658:1682	The coated sponge surface	1658:1682	The coated sponge surface has provided a suitable moist-adherent physical environment to support the adhesion and growth of skin cells (HaCaT cells).
33254970	3	14	theme	vegetable	533:541	arg1	BioPU					571:575	BioPU	571:575	BioPU	571:575	These sponges were modified by employing a simple coating strategy using vegetable oil-based bio-polyurethane (BioPU) to tailor their physicochemical and biological properties so as to match the specific requirements of a wound care scaffold.
33254970	3	14	theme	vegetable	533:541	arg1	bio-polyurethane					553:568	vegetable oil-based bio-polyurethane	533:568	vegetable oil-based bio-polyurethane (BioPU)	533:576	These sponges were modified by employing a simple coating strategy using vegetable oil-based bio-polyurethane (BioPU) to tailor their physicochemical and biological properties so as to match the specific requirements of a wound care scaffold.
33254970	5	15	theme	BioPU	851:855	arg1	chains					857:862	BioPU chains	851:862	BioPU chains	851:862	The interpenetration of BioPU chains within the sponge construct resulted in the formation of numerous micro-networks in the interconnected microporous structure of sponges (porosity ≥75%).
33254970	9	16	theme	coated	1662:1667	arg1	surface					1676:1682	The coated sponge surface	1658:1682	The coated sponge surface	1658:1682	The coated sponge surface has provided a suitable moist-adherent physical environment to support the adhesion and growth of skin cells (HaCaT cells).
33254970	11	17	theme	Dawley	2125:2130	arg1	rats					2137:2140	Sprague Dawley (SD) rats	2117:2140	Sprague Dawley (SD) rats	2117:2140	Moreover, no signs of skin erythema or edema were observed during in vivo dermal irritation and corrosion test performed on the skin of Sprague Dawley (SD) rats.
33254970	7	18	theme	sponge	1391:1396	arg1	construct					1398:1406	the sponge construct	1387:1406	the sponge construct	1387:1406	A tunable degradation behaviour was achieved by varying BioPU coating concentrations owing to the different degree of polymer chain entanglement within the sponge construct.
33254970	11	19	theme	in	2047:2048	arg1	irritation					2062:2071	in vivo dermal irritation	2047:2071	in vivo dermal irritation	2047:2071	Moreover, no signs of skin erythema or edema were observed during in vivo dermal irritation and corrosion test performed on the skin of Sprague Dawley (SD) rats.
33254970	11	20	theme	erythema	2008:2015	arg1	signs					1994:1998	no signs	1991:1998	no signs of skin erythema or edema	1991:2024	Moreover, no signs of skin erythema or edema were observed during in vivo dermal irritation and corrosion test performed on the skin of Sprague Dawley (SD) rats.
33254970	5	21	theme	micro-networks	930:943	arg1	formation					908:916	the formation	904:916	the formation of numerous micro-networks in the interconnected microporous structure of sponges (porosity ≥75%)	904:1014	The interpenetration of BioPU chains within the sponge construct resulted in the formation of numerous micro-networks in the interconnected microporous structure of sponges (porosity ≥75%).
33254970	11	22	theme	rats	2137:2140	arg1	skin					2109:2112	the skin	2105:2112	the skin of Sprague Dawley (SD) rats	2105:2140	Moreover, no signs of skin erythema or edema were observed during in vivo dermal irritation and corrosion test performed on the skin of Sprague Dawley (SD) rats.
33254970	10	23	theme	biocompatible	1932:1944	arg1	nature					1946:1951	the non-toxic and biocompatible nature	1914:1951	the non-toxic and biocompatible nature of coated sponges	1914:1969	The MTT (3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide) assay and hemolytic assay revealed the non-toxic and biocompatible nature of coated sponges in vitro.
33254970	12	24	theme	initial	2147:2153	arg1	observations					2155:2166	Our initial observations	2143:2166	Our initial observations	2143:2166	Our initial observations revealed the credibility of these sponges as functional wound care scaffolds as well as its diverse potential as a suitable substrate for various tissue engineering applications.
33254970	8	25	theme	core	1456:1459	arg1	components					1472:1481	core structural components	1456:1481	core structural components of sponge	1456:1491	The physical entanglement of BioPU chains with core structural components of sponge improved their structural stability by suppressing their full fragmentation in water-based medium without affecting its swelling behaviour (swelling ratio > 1000%).
33254970	3	26	theme	coating	510:516	arg1	strategy					518:525	a simple coating strategy	501:525	a simple coating strategy using vegetable oil-based bio-polyurethane (BioPU) to tailor their physicochemical and biological properties so as to match the specific requirements of a wound care scaffold	501:700	These sponges were modified by employing a simple coating strategy using vegetable oil-based bio-polyurethane (BioPU) to tailor their physicochemical and biological properties so as to match the specific requirements of a wound care scaffold.
33254970	8	27	theme	water-based	1572:1582	arg1	medium					1584:1589	water-based medium	1572:1589	water-based medium	1572:1589	The physical entanglement of BioPU chains with core structural components of sponge improved their structural stability by suppressing their full fragmentation in water-based medium without affecting its swelling behaviour (swelling ratio > 1000%).
33254970	7	28	theme	BioPU	1291:1295	arg1	concentrations					1305:1318	BioPU coating concentrations	1291:1318	BioPU coating concentrations owing to the different degree of polymer chain entanglement within the sponge construct	1291:1406	A tunable degradation behaviour was achieved by varying BioPU coating concentrations owing to the different degree of polymer chain entanglement within the sponge construct.
33254970	9	29	dep	adhesion	1759:1766	arg1	the					1755:1757	the	1755:1757	the	1755:1757	The coated sponge surface has provided a suitable moist-adherent physical environment to support the adhesion and growth of skin cells (HaCaT cells).
33254970	6	30	theme	coated	1021:1026	arg1	sponge					1028:1033	The coated sponge	1017:1033	The coated sponge	1017:1033	The coated sponge showed a superior mechanical strength (compressive strength ~3.8 MPa, compressive modulus ~35 MPa) with appreciable flexibility and recoverability under repeated compressive loading-unloading cycles.
33254970	5	31	from	formation	908:916	arg1	structure					979:987	the interconnected microporous structure	948:987	the interconnected microporous structure of sponges (porosity ≥75%)	948:1014	The interpenetration of BioPU chains within the sponge construct resulted in the formation of numerous micro-networks in the interconnected microporous structure of sponges (porosity ≥75%).
33254970	8	32	with	chains	1444:1449	arg1	components					1472:1481	core structural components	1456:1481	core structural components of sponge	1456:1491	The physical entanglement of BioPU chains with core structural components of sponge improved their structural stability by suppressing their full fragmentation in water-based medium without affecting its swelling behaviour (swelling ratio > 1000%).
33254970	0	33	theme	potential	117:125	arg1	scaffolds					138:146	potential wound care scaffolds	117:146	potential wound care scaffolds	117:146	Bio-extract amalgamated sodium alginate-cellulose nanofibres based 3D-sponges with interpenetrating BioPU coating as potential wound care scaffolds.
33254970	10	34	theme	thiazolyl-2	1833:1843	arg1	bromide					1870:1876	3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide	1817:1876	3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide	1817:1876	The MTT (3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide) assay and hemolytic assay revealed the non-toxic and biocompatible nature of coated sponges in vitro.
33254970	10	34	theme	thiazolyl-2	1833:1843	arg1	MTT					1812:1814	The MTT	1808:1814	The MTT (3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide) assay	1808:1883	The MTT (3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide) assay and hemolytic assay revealed the non-toxic and biocompatible nature of coated sponges in vitro.
33254970	12	35	theme	diverse	2260:2266	arg1	potential					2268:2276	its diverse potential	2256:2276	functional wound care scaffolds as well as its diverse potential	2213:2276	Our initial observations revealed the credibility of these sponges as functional wound care scaffolds as well as its diverse potential as a suitable substrate for various tissue engineering applications.
33254970	12	36	theme	tissue	2314:2319	arg1	applications					2333:2344	various tissue engineering applications	2306:2344	various tissue engineering applications	2306:2344	Our initial observations revealed the credibility of these sponges as functional wound care scaffolds as well as its diverse potential as a suitable substrate for various tissue engineering applications.
33254970	11	37	theme	edema	2020:2024	arg1	signs					1994:1998	no signs	1991:1998	no signs of skin erythema or edema	1991:2024	Moreover, no signs of skin erythema or edema were observed during in vivo dermal irritation and corrosion test performed on the skin of Sprague Dawley (SD) rats.
33254970	12	38	theme	various	2306:2312	arg1	applications					2333:2344	various tissue engineering applications	2306:2344	various tissue engineering applications	2306:2344	Our initial observations revealed the credibility of these sponges as functional wound care scaffolds as well as its diverse potential as a suitable substrate for various tissue engineering applications.
33254970	0	39	theme	care	133:136	arg1	scaffolds					138:146	potential wound care scaffolds	117:146	potential wound care scaffolds	117:146	Bio-extract amalgamated sodium alginate-cellulose nanofibres based 3D-sponges with interpenetrating BioPU coating as potential wound care scaffolds.
33254970	4	40	theme	shared	720:725	arg1	structures					762:771	shared interpenetrating polymeric network structures	720:771	shared interpenetrating polymeric network structures	720:771	Bio-sponges with shared interpenetrating polymeric network structures were attained at optimized BioPU coating formulation.
33254970	0	41	theme	Bio-extract	0:10	arg1	alginate-cellulose					31:48	Bio-extract amalgamated sodium alginate-cellulose	0:48	Bio-extract amalgamated sodium alginate-cellulose	0:48	Bio-extract amalgamated sodium alginate-cellulose nanofibres based 3D-sponges with interpenetrating BioPU coating as potential wound care scaffolds.
33254970	5	42	theme	microporous	967:977	arg1	structure					979:987	the interconnected microporous structure	948:987	the interconnected microporous structure of sponges (porosity ≥75%)	948:1014	The interpenetration of BioPU chains within the sponge construct resulted in the formation of numerous micro-networks in the interconnected microporous structure of sponges (porosity ≥75%).
33254970	6	43	theme	compressive	1074:1084	arg1	~35 MPa					1125:1131	compressive modulus ~35 MPa	1105:1131	compressive modulus ~35 MPa	1105:1131	The coated sponge showed a superior mechanical strength (compressive strength ~3.8 MPa, compressive modulus ~35 MPa) with appreciable flexibility and recoverability under repeated compressive loading-unloading cycles.
33254970	6	43	theme	compressive	1074:1084	arg1	~3.8 MPa					1095:1102	compressive strength ~3.8 MPa	1074:1102	compressive strength ~3.8 MPa	1074:1102	The coated sponge showed a superior mechanical strength (compressive strength ~3.8 MPa, compressive modulus ~35 MPa) with appreciable flexibility and recoverability under repeated compressive loading-unloading cycles.
33254970	4	44	theme	polymeric	744:752	arg1	structures					762:771	shared interpenetrating polymeric network structures	720:771	shared interpenetrating polymeric network structures	720:771	Bio-sponges with shared interpenetrating polymeric network structures were attained at optimized BioPU coating formulation.
33254970	0	45	theme	sodium	24:29	arg1	alginate-cellulose					31:48	Bio-extract amalgamated sodium alginate-cellulose	0:48	Bio-extract amalgamated sodium alginate-cellulose	0:48	Bio-extract amalgamated sodium alginate-cellulose nanofibres based 3D-sponges with interpenetrating BioPU coating as potential wound care scaffolds.
33254970	11	46	theme	corrosion	2077:2085	arg1	test					2087:2090	corrosion test	2077:2090	corrosion test	2077:2090	Moreover, no signs of skin erythema or edema were observed during in vivo dermal irritation and corrosion test performed on the skin of Sprague Dawley (SD) rats.
33254970	6	47	theme	mechanical	1053:1062	arg1	strength					1064:1071	a superior mechanical strength	1042:1071	a superior mechanical strength (compressive strength ~3.8 MPa, compressive modulus ~35 MPa)	1042:1132	The coated sponge showed a superior mechanical strength (compressive strength ~3.8 MPa, compressive modulus ~35 MPa) with appreciable flexibility and recoverability under repeated compressive loading-unloading cycles.
33254970	11	48	theme	skin	2003:2006	arg1	erythema					2008:2015	skin erythema	2003:2015	skin erythema	2003:2015	Moreover, no signs of skin erythema or edema were observed during in vivo dermal irritation and corrosion test performed on the skin of Sprague Dawley (SD) rats.
33254970	6	49	theme	superior	1044:1051	arg1	strength					1064:1071	a superior mechanical strength	1042:1071	a superior mechanical strength (compressive strength ~3.8 MPa, compressive modulus ~35 MPa)	1042:1132	The coated sponge showed a superior mechanical strength (compressive strength ~3.8 MPa, compressive modulus ~35 MPa) with appreciable flexibility and recoverability under repeated compressive loading-unloading cycles.
33254970	0	50	theme	interpenetrating	83:98	arg1	BioPU					100:104	interpenetrating BioPU	83:104	interpenetrating BioPU coating as potential wound care scaffolds	83:146	Bio-extract amalgamated sodium alginate-cellulose nanofibres based 3D-sponges with interpenetrating BioPU coating as potential wound care scaffolds.
33254970	8	51	theme	sponge	1486:1491	arg1	components					1472:1481	core structural components	1456:1481	core structural components of sponge	1456:1491	The physical entanglement of BioPU chains with core structural components of sponge improved their structural stability by suppressing their full fragmentation in water-based medium without affecting its swelling behaviour (swelling ratio > 1000%).
33254970	10	52	theme	coated	1956:1961	arg1	sponges					1963:1969	coated sponges	1956:1969	coated sponges	1956:1969	The MTT (3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide) assay and hemolytic assay revealed the non-toxic and biocompatible nature of coated sponges in vitro.
33254970	0	53	dep	nanofibres	50:59	arg1	based					61:65	based	61:65	nanofibres based 3D-sponges with interpenetrating BioPU coating as potential wound care scaffolds	50:146	Bio-extract amalgamated sodium alginate-cellulose nanofibres based 3D-sponges with interpenetrating BioPU coating as potential wound care scaffolds.
33254970	11	54	theme	dermal	2055:2060	arg1	irritation					2062:2071	in vivo dermal irritation	2047:2071	in vivo dermal irritation	2047:2071	Moreover, no signs of skin erythema or edema were observed during in vivo dermal irritation and corrosion test performed on the skin of Sprague Dawley (SD) rats.
33254970	3	55	theme	specific	655:662	arg1	requirements					664:675	the specific requirements	651:675	the specific requirements of a wound care scaffold	651:700	These sponges were modified by employing a simple coating strategy using vegetable oil-based bio-polyurethane (BioPU) to tailor their physicochemical and biological properties so as to match the specific requirements of a wound care scaffold.
33254970	4	56	theme	optimized	790:798	arg1	formulation					814:824	optimized BioPU coating formulation	790:824	optimized BioPU coating formulation	790:824	Bio-sponges with shared interpenetrating polymeric network structures were attained at optimized BioPU coating formulation.
33254970	2	57	theme	aqueous	340:346	arg1	amalgamation					348:359	the aqueous amalgamation	336:359	the aqueous amalgamation of SA and cellulose nanofibres (CNFs) in bio-extracts like Rice water (Rw) and Giloy extract (Ge)	336:457	The composite bio-sponges were developed from the aqueous amalgamation of SA and cellulose nanofibres (CNFs) in bio-extracts like Rice water (Rw) and Giloy extract (Ge).
33254970	7	58	theme	different	1333:1341	arg1	degree					1343:1348	the different degree	1329:1348	the different degree of polymer chain entanglement within the sponge construct	1329:1406	A tunable degradation behaviour was achieved by varying BioPU coating concentrations owing to the different degree of polymer chain entanglement within the sponge construct.
33254970	7	59	theme	chain	1361:1365	arg1	degree					1343:1348	the different degree	1329:1348	the different degree of polymer chain entanglement within the sponge construct	1329:1406	A tunable degradation behaviour was achieved by varying BioPU coating concentrations owing to the different degree of polymer chain entanglement within the sponge construct.
33254970	4	60	theme	coating	806:812	arg1	formulation					814:824	optimized BioPU coating formulation	790:824	optimized BioPU coating formulation	790:824	Bio-sponges with shared interpenetrating polymeric network structures were attained at optimized BioPU coating formulation.
33254970	2	61	theme	nanofibres	381:390	arg1	amalgamation					348:359	the aqueous amalgamation	336:359	the aqueous amalgamation of SA and cellulose nanofibres (CNFs) in bio-extracts like Rice water (Rw) and Giloy extract (Ge)	336:457	The composite bio-sponges were developed from the aqueous amalgamation of SA and cellulose nanofibres (CNFs) in bio-extracts like Rice water (Rw) and Giloy extract (Ge).
33254970	3	62	theme	wound	682:686	arg1	scaffold					693:700	a wound care scaffold	680:700	a wound care scaffold	680:700	These sponges were modified by employing a simple coating strategy using vegetable oil-based bio-polyurethane (BioPU) to tailor their physicochemical and biological properties so as to match the specific requirements of a wound care scaffold.
33254970	10	63	theme	MTT	1812:1814	arg1	assay					1879:1883	The MTT (3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide) assay	1808:1883	The MTT (3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide) assay	1808:1883	The MTT (3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide) assay and hemolytic assay revealed the non-toxic and biocompatible nature of coated sponges in vitro.
33254970	6	64	theme	compressive	1105:1115	arg1	~35 MPa					1125:1131	compressive modulus ~35 MPa	1105:1131	compressive modulus ~35 MPa	1105:1131	The coated sponge showed a superior mechanical strength (compressive strength ~3.8 MPa, compressive modulus ~35 MPa) with appreciable flexibility and recoverability under repeated compressive loading-unloading cycles.
33254970	6	64	theme	compressive	1105:1115	arg1	~3.8 MPa					1095:1102	compressive strength ~3.8 MPa	1074:1102	compressive strength ~3.8 MPa	1074:1102	The coated sponge showed a superior mechanical strength (compressive strength ~3.8 MPa, compressive modulus ~35 MPa) with appreciable flexibility and recoverability under repeated compressive loading-unloading cycles.
33254970	2	65	theme	composite	294:302	arg1	bio-sponges					304:314	The composite bio-sponges	290:314	The composite bio-sponges	290:314	The composite bio-sponges were developed from the aqueous amalgamation of SA and cellulose nanofibres (CNFs) in bio-extracts like Rice water (Rw) and Giloy extract (Ge).
33254970	1	66	theme	wound	269:273	arg1	scaffold					280:287	wound care scaffold	269:287	wound care scaffold	269:287	In this work, sodium alginate (SA) based "all-natural" composite bio-sponges were designed for potential application as wound care scaffold.
33254970	10	67	theme	4,5-dimethyl	1820:1831	arg1	bromide					1870:1876	3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide	1817:1876	3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide	1817:1876	The MTT (3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide) assay and hemolytic assay revealed the non-toxic and biocompatible nature of coated sponges in vitro.
33254970	10	67	theme	4,5-dimethyl	1820:1831	arg1	MTT					1812:1814	The MTT	1808:1814	The MTT (3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide) assay	1808:1883	The MTT (3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide) assay and hemolytic assay revealed the non-toxic and biocompatible nature of coated sponges in vitro.
33254970	9	68	theme	skin	1782:1785	arg1	cells					1787:1791	skin cells	1782:1791	skin cells (HaCaT cells)	1782:1805	The coated sponge surface has provided a suitable moist-adherent physical environment to support the adhesion and growth of skin cells (HaCaT cells).
33254970	9	68	theme	skin	1782:1785	arg1	cells					1800:1804	HaCaT cells	1794:1804	HaCaT cells	1794:1804	The coated sponge surface has provided a suitable moist-adherent physical environment to support the adhesion and growth of skin cells (HaCaT cells).
33254970	6	69	theme	loading-unloading	1209:1225	arg1	cycles					1227:1232	repeated compressive loading-unloading cycles	1188:1232	repeated compressive loading-unloading cycles	1188:1232	The coated sponge showed a superior mechanical strength (compressive strength ~3.8 MPa, compressive modulus ~35 MPa) with appreciable flexibility and recoverability under repeated compressive loading-unloading cycles.
33254970	3	70	theme	scaffold	693:700	arg1	requirements					664:675	the specific requirements	651:675	the specific requirements of a wound care scaffold	651:700	These sponges were modified by employing a simple coating strategy using vegetable oil-based bio-polyurethane (BioPU) to tailor their physicochemical and biological properties so as to match the specific requirements of a wound care scaffold.
33254970	3	71	theme	oil-based	543:551	arg1	BioPU					571:575	BioPU	571:575	BioPU	571:575	These sponges were modified by employing a simple coating strategy using vegetable oil-based bio-polyurethane (BioPU) to tailor their physicochemical and biological properties so as to match the specific requirements of a wound care scaffold.
33254970	3	71	theme	oil-based	543:551	arg1	bio-polyurethane					553:568	vegetable oil-based bio-polyurethane	533:568	vegetable oil-based bio-polyurethane (BioPU)	533:576	These sponges were modified by employing a simple coating strategy using vegetable oil-based bio-polyurethane (BioPU) to tailor their physicochemical and biological properties so as to match the specific requirements of a wound care scaffold.
33254970	2	72	from	nanofibres	381:390	arg1	bio-extracts					402:413	bio-extracts	402:413	bio-extracts like Rice water (Rw) and Giloy extract (Ge)	402:457	The composite bio-sponges were developed from the aqueous amalgamation of SA and cellulose nanofibres (CNFs) in bio-extracts like Rice water (Rw) and Giloy extract (Ge).
33254970	6	73	theme	repeated	1188:1195	arg1	cycles					1227:1232	repeated compressive loading-unloading cycles	1188:1232	repeated compressive loading-unloading cycles	1188:1232	The coated sponge showed a superior mechanical strength (compressive strength ~3.8 MPa, compressive modulus ~35 MPa) with appreciable flexibility and recoverability under repeated compressive loading-unloading cycles.
33254970	12	74	theme	sponges	2202:2208	arg1	scaffolds					2235:2243	functional wound care scaffolds	2213:2243	functional wound care scaffolds as well as its diverse potential	2213:2276	Our initial observations revealed the credibility of these sponges as functional wound care scaffolds as well as its diverse potential as a suitable substrate for various tissue engineering applications.
33254970	12	74	theme	sponges	2202:2208	arg1	substrate					2292:2300	a suitable substrate	2281:2300	a suitable substrate for various tissue engineering applications	2281:2344	Our initial observations revealed the credibility of these sponges as functional wound care scaffolds as well as its diverse potential as a suitable substrate for various tissue engineering applications.
33254970	12	74	theme	sponges	2202:2208	arg1	credibility					2181:2191	the credibility	2177:2191	the credibility of these sponges	2177:2208	Our initial observations revealed the credibility of these sponges as functional wound care scaffolds as well as its diverse potential as a suitable substrate for various tissue engineering applications.
33254970	9	75	theme	moist-adherent	1708:1721	arg1	environment					1732:1742	a suitable moist-adherent physical environment	1697:1742	a suitable moist-adherent physical environment to support the adhesion and growth of skin cells (HaCaT cells)	1697:1805	The coated sponge surface has provided a suitable moist-adherent physical environment to support the adhesion and growth of skin cells (HaCaT cells).
33254970	1	76	theme	all-natural	191:201	arg1	bio-sponges					214:224	sodium alginate (SA) based "all-natural" composite bio-sponges	163:224	sodium alginate (SA) based "all-natural" composite bio-sponges	163:224	In this work, sodium alginate (SA) based "all-natural" composite bio-sponges were designed for potential application as wound care scaffold.
33254970	5	77	theme	chains	857:862	arg1	interpenetration					831:846	The interpenetration	827:846	The interpenetration of BioPU chains within the sponge construct	827:890	The interpenetration of BioPU chains within the sponge construct resulted in the formation of numerous micro-networks in the interconnected microporous structure of sponges (porosity ≥75%).
33254970	8	78	theme	full	1550:1553	arg1	fragmentation					1555:1567	their full fragmentation	1544:1567	their full fragmentation	1544:1567	The physical entanglement of BioPU chains with core structural components of sponge improved their structural stability by suppressing their full fragmentation in water-based medium without affecting its swelling behaviour (swelling ratio > 1000%).
33254970	10	79	theme	hemolytic	1889:1897	arg1	assay					1899:1903	hemolytic assay	1889:1903	hemolytic assay	1889:1903	The MTT (3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide) assay and hemolytic assay revealed the non-toxic and biocompatible nature of coated sponges in vitro.
33254970	5	80	theme	numerous	921:928	arg1	micro-networks					930:943	numerous micro-networks	921:943	numerous micro-networks in the interconnected microporous structure of sponges (porosity ≥75%)	921:1014	The interpenetration of BioPU chains within the sponge construct resulted in the formation of numerous micro-networks in the interconnected microporous structure of sponges (porosity ≥75%).
33254970	11	81	theme	SD	2133:2134	arg1	rats					2137:2140	Sprague Dawley (SD) rats	2117:2140	Sprague Dawley (SD) rats	2117:2140	Moreover, no signs of skin erythema or edema were observed during in vivo dermal irritation and corrosion test performed on the skin of Sprague Dawley (SD) rats.
33254970	12	82	theme	wound	2224:2228	arg1	scaffolds					2235:2243	functional wound care scaffolds	2213:2243	functional wound care scaffolds as well as its diverse potential	2213:2276	Our initial observations revealed the credibility of these sponges as functional wound care scaffolds as well as its diverse potential as a suitable substrate for various tissue engineering applications.
33254970	12	82	theme	wound	2224:2228	arg1	credibility					2181:2191	the credibility	2177:2191	the credibility of these sponges	2177:2208	Our initial observations revealed the credibility of these sponges as functional wound care scaffolds as well as its diverse potential as a suitable substrate for various tissue engineering applications.
33254970	3	83	theme	physicochemical	594:608	arg1	properties					625:634	their physicochemical and biological properties	588:634	their physicochemical and biological properties so as to match the specific requirements of a wound care scaffold	588:700	These sponges were modified by employing a simple coating strategy using vegetable oil-based bio-polyurethane (BioPU) to tailor their physicochemical and biological properties so as to match the specific requirements of a wound care scaffold.
33254970	10	84	theme	non-toxic	1918:1926	arg1	nature					1946:1951	the non-toxic and biocompatible nature	1914:1951	the non-toxic and biocompatible nature of coated sponges	1914:1969	The MTT (3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide) assay and hemolytic assay revealed the non-toxic and biocompatible nature of coated sponges in vitro.
33254970	12	85	theme	care	2230:2233	arg1	scaffolds					2235:2243	functional wound care scaffolds	2213:2243	functional wound care scaffolds as well as its diverse potential	2213:2276	Our initial observations revealed the credibility of these sponges as functional wound care scaffolds as well as its diverse potential as a suitable substrate for various tissue engineering applications.
33254970	12	85	theme	care	2230:2233	arg1	credibility					2181:2191	the credibility	2177:2191	the credibility of these sponges	2177:2208	Our initial observations revealed the credibility of these sponges as functional wound care scaffolds as well as its diverse potential as a suitable substrate for various tissue engineering applications.
33254970	8	86	theme	swelling	1613:1620	arg1	%					1654:1654	swelling ratio > 1000%	1633:1654	swelling ratio > 1000%	1633:1654	The physical entanglement of BioPU chains with core structural components of sponge improved their structural stability by suppressing their full fragmentation in water-based medium without affecting its swelling behaviour (swelling ratio > 1000%).
33254970	8	86	theme	swelling	1613:1620	arg1	behaviour					1622:1630	its swelling behaviour	1609:1630	its swelling behaviour (swelling ratio > 1000%)	1609:1655	The physical entanglement of BioPU chains with core structural components of sponge improved their structural stability by suppressing their full fragmentation in water-based medium without affecting its swelling behaviour (swelling ratio > 1000%).
33254970	0	87	theme	wound	127:131	arg1	scaffolds					138:146	potential wound care scaffolds	117:146	potential wound care scaffolds	117:146	Bio-extract amalgamated sodium alginate-cellulose nanofibres based 3D-sponges with interpenetrating BioPU coating as potential wound care scaffolds.
33254970	5	88	theme	sponges	992:998	arg1	structure					979:987	the interconnected microporous structure	948:987	the interconnected microporous structure of sponges (porosity ≥75%)	948:1014	The interpenetration of BioPU chains within the sponge construct resulted in the formation of numerous micro-networks in the interconnected microporous structure of sponges (porosity ≥75%).
33254970	10	89	theme	-2,5-diphenyltetrazolium	1845:1868	arg1	bromide					1870:1876	3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide	1817:1876	3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide	1817:1876	The MTT (3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide) assay and hemolytic assay revealed the non-toxic and biocompatible nature of coated sponges in vitro.
33254970	10	89	theme	-2,5-diphenyltetrazolium	1845:1868	arg1	MTT					1812:1814	The MTT	1808:1814	The MTT (3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide) assay	1808:1883	The MTT (3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide) assay and hemolytic assay revealed the non-toxic and biocompatible nature of coated sponges in vitro.
33254970	12	90	theme	engineering	2321:2331	arg1	applications					2333:2344	various tissue engineering applications	2306:2344	various tissue engineering applications	2306:2344	Our initial observations revealed the credibility of these sponges as functional wound care scaffolds as well as its diverse potential as a suitable substrate for various tissue engineering applications.
33254970	12	91	theme	suitable	2283:2290	arg1	substrate					2292:2300	a suitable substrate	2281:2300	a suitable substrate for various tissue engineering applications	2281:2344	Our initial observations revealed the credibility of these sponges as functional wound care scaffolds as well as its diverse potential as a suitable substrate for various tissue engineering applications.
33254970	12	91	theme	suitable	2283:2290	arg1	credibility					2181:2191	the credibility	2177:2191	the credibility of these sponges	2177:2208	Our initial observations revealed the credibility of these sponges as functional wound care scaffolds as well as its diverse potential as a suitable substrate for various tissue engineering applications.
33254970	4	92	theme	network	754:760	arg1	structures					762:771	shared interpenetrating polymeric network structures	720:771	shared interpenetrating polymeric network structures	720:771	Bio-sponges with shared interpenetrating polymeric network structures were attained at optimized BioPU coating formulation.
33254970	7	93	theme	tunable	1237:1243	arg1	behaviour					1257:1265	A tunable degradation behaviour	1235:1265	A tunable degradation behaviour	1235:1265	A tunable degradation behaviour was achieved by varying BioPU coating concentrations owing to the different degree of polymer chain entanglement within the sponge construct.
33254970	1	94	theme	"	202:202	arg1	bio-sponges					214:224	sodium alginate (SA) based "all-natural" composite bio-sponges	163:224	sodium alginate (SA) based "all-natural" composite bio-sponges	163:224	In this work, sodium alginate (SA) based "all-natural" composite bio-sponges were designed for potential application as wound care scaffold.
33254970	5	95	theme	porosity	1001:1008	arg1	%					1013:1013	porosity ≥75%	1001:1013	porosity ≥75%	1001:1013	The interpenetration of BioPU chains within the sponge construct resulted in the formation of numerous micro-networks in the interconnected microporous structure of sponges (porosity ≥75%).
33254970	5	95	theme	porosity	1001:1008	arg1	sponges					992:998	sponges	992:998	sponges (porosity ≥75%)	992:1014	The interpenetration of BioPU chains within the sponge construct resulted in the formation of numerous micro-networks in the interconnected microporous structure of sponges (porosity ≥75%).
33254970	6	96	dep	strength	1064:1071	arg1	~35 MPa					1125:1131	compressive modulus ~35 MPa	1105:1131	compressive modulus ~35 MPa	1105:1131	The coated sponge showed a superior mechanical strength (compressive strength ~3.8 MPa, compressive modulus ~35 MPa) with appreciable flexibility and recoverability under repeated compressive loading-unloading cycles.
33254970	6	96	dep	strength	1064:1071	arg1	~3.8 MPa					1095:1102	compressive strength ~3.8 MPa	1074:1102	compressive strength ~3.8 MPa	1074:1102	The coated sponge showed a superior mechanical strength (compressive strength ~3.8 MPa, compressive modulus ~35 MPa) with appreciable flexibility and recoverability under repeated compressive loading-unloading cycles.
33254970	0	97	theme	amalgamated	12:22	arg1	alginate-cellulose					31:48	Bio-extract amalgamated sodium alginate-cellulose	0:48	Bio-extract amalgamated sodium alginate-cellulose	0:48	Bio-extract amalgamated sodium alginate-cellulose nanofibres based 3D-sponges with interpenetrating BioPU coating as potential wound care scaffolds.
33254970	5	98	theme	interconnected	952:965	arg1	structure					979:987	the interconnected microporous structure	948:987	the interconnected microporous structure of sponges (porosity ≥75%)	948:1014	The interpenetration of BioPU chains within the sponge construct resulted in the formation of numerous micro-networks in the interconnected microporous structure of sponges (porosity ≥75%).
33254970	6	99	theme	strength	1086:1093	arg1	~35 MPa					1125:1131	compressive modulus ~35 MPa	1105:1131	compressive modulus ~35 MPa	1105:1131	The coated sponge showed a superior mechanical strength (compressive strength ~3.8 MPa, compressive modulus ~35 MPa) with appreciable flexibility and recoverability under repeated compressive loading-unloading cycles.
33254970	6	99	theme	strength	1086:1093	arg1	~3.8 MPa					1095:1102	compressive strength ~3.8 MPa	1074:1102	compressive strength ~3.8 MPa	1074:1102	The coated sponge showed a superior mechanical strength (compressive strength ~3.8 MPa, compressive modulus ~35 MPa) with appreciable flexibility and recoverability under repeated compressive loading-unloading cycles.
33254970	8	100	theme	BioPU	1438:1442	arg1	chains					1444:1449	BioPU chains	1438:1449	BioPU chains with core structural components of sponge	1438:1491	The physical entanglement of BioPU chains with core structural components of sponge improved their structural stability by suppressing their full fragmentation in water-based medium without affecting its swelling behaviour (swelling ratio > 1000%).
33254970	5	101	from	micro-networks	930:943	arg1	structure					979:987	the interconnected microporous structure	948:987	the interconnected microporous structure of sponges (porosity ≥75%)	948:1014	The interpenetration of BioPU chains within the sponge construct resulted in the formation of numerous micro-networks in the interconnected microporous structure of sponges (porosity ≥75%).
33254970	7	102	theme	entanglement	1367:1378	arg1	degree					1343:1348	the different degree	1329:1348	the different degree of polymer chain entanglement within the sponge construct	1329:1406	A tunable degradation behaviour was achieved by varying BioPU coating concentrations owing to the different degree of polymer chain entanglement within the sponge construct.
33254970	10	103	theme	sponges	1963:1969	arg1	nature					1946:1951	the non-toxic and biocompatible nature	1914:1951	the non-toxic and biocompatible nature of coated sponges	1914:1969	The MTT (3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide) assay and hemolytic assay revealed the non-toxic and biocompatible nature of coated sponges in vitro.
33254970	4	104	theme	interpenetrating	727:742	arg1	structures					762:771	shared interpenetrating polymeric network structures	720:771	shared interpenetrating polymeric network structures	720:771	Bio-sponges with shared interpenetrating polymeric network structures were attained at optimized BioPU coating formulation.
33254970	2	105	theme	Rice	420:423	arg1	Rw					432:433	Rw	432:433	Rw	432:433	The composite bio-sponges were developed from the aqueous amalgamation of SA and cellulose nanofibres (CNFs) in bio-extracts like Rice water (Rw) and Giloy extract (Ge).
33254970	2	105	theme	Rice	420:423	arg1	water					425:429	Rice water	420:429	Rice water (Rw)	420:434	The composite bio-sponges were developed from the aqueous amalgamation of SA and cellulose nanofibres (CNFs) in bio-extracts like Rice water (Rw) and Giloy extract (Ge).
33254970	1	106	theme	composite	204:212	arg1	bio-sponges					214:224	sodium alginate (SA) based "all-natural" composite bio-sponges	163:224	sodium alginate (SA) based "all-natural" composite bio-sponges	163:224	In this work, sodium alginate (SA) based "all-natural" composite bio-sponges were designed for potential application as wound care scaffold.
33254970	7	107	theme	polymer	1353:1359	arg1	chain					1361:1365	polymer chain	1353:1365	polymer chain	1353:1365	A tunable degradation behaviour was achieved by varying BioPU coating concentrations owing to the different degree of polymer chain entanglement within the sponge construct.
33254970	3	108	theme	biological	614:623	arg1	properties					625:634	their physicochemical and biological properties	588:634	their physicochemical and biological properties so as to match the specific requirements of a wound care scaffold	588:700	These sponges were modified by employing a simple coating strategy using vegetable oil-based bio-polyurethane (BioPU) to tailor their physicochemical and biological properties so as to match the specific requirements of a wound care scaffold.
33254970	8	109	theme	structural	1461:1470	arg1	components					1472:1481	core structural components	1456:1481	core structural components of sponge	1456:1491	The physical entanglement of BioPU chains with core structural components of sponge improved their structural stability by suppressing their full fragmentation in water-based medium without affecting its swelling behaviour (swelling ratio > 1000%).
33254970	7	110	theme	degradation	1245:1255	arg1	behaviour					1257:1265	A tunable degradation behaviour	1235:1265	A tunable degradation behaviour	1235:1265	A tunable degradation behaviour was achieved by varying BioPU coating concentrations owing to the different degree of polymer chain entanglement within the sponge construct.
33254970	9	111	theme	suitable	1699:1706	arg1	environment					1732:1742	a suitable moist-adherent physical environment	1697:1742	a suitable moist-adherent physical environment to support the adhesion and growth of skin cells (HaCaT cells)	1697:1805	The coated sponge surface has provided a suitable moist-adherent physical environment to support the adhesion and growth of skin cells (HaCaT cells).
33254970	2	112	theme	Giloy	440:444	arg1	extract					446:452	Giloy extract	440:452	Giloy extract (Ge)	440:457	The composite bio-sponges were developed from the aqueous amalgamation of SA and cellulose nanofibres (CNFs) in bio-extracts like Rice water (Rw) and Giloy extract (Ge).
33254970	2	112	theme	Giloy	440:444	arg1	Ge					455:456	Ge	455:456	Ge	455:456	The composite bio-sponges were developed from the aqueous amalgamation of SA and cellulose nanofibres (CNFs) in bio-extracts like Rice water (Rw) and Giloy extract (Ge).
33254970	5	113	from	structure	979:987	arg1	formation					908:916	the formation	904:916	the formation of numerous micro-networks in the interconnected microporous structure of sponges (porosity ≥75%)	904:1014	The interpenetration of BioPU chains within the sponge construct resulted in the formation of numerous micro-networks in the interconnected microporous structure of sponges (porosity ≥75%).
33254970	4	114	theme	BioPU	800:804	arg1	formulation					814:824	optimized BioPU coating formulation	790:824	optimized BioPU coating formulation	790:824	Bio-sponges with shared interpenetrating polymeric network structures were attained at optimized BioPU coating formulation.
33254970	9	115	theme	physical	1723:1730	arg1	environment					1732:1742	a suitable moist-adherent physical environment	1697:1742	a suitable moist-adherent physical environment to support the adhesion and growth of skin cells (HaCaT cells)	1697:1805	The coated sponge surface has provided a suitable moist-adherent physical environment to support the adhesion and growth of skin cells (HaCaT cells).
33254970	6	116	theme	appreciable	1139:1149	arg1	flexibility					1151:1161	appreciable flexibility	1139:1161	appreciable flexibility	1139:1161	The coated sponge showed a superior mechanical strength (compressive strength ~3.8 MPa, compressive modulus ~35 MPa) with appreciable flexibility and recoverability under repeated compressive loading-unloading cycles.
33254970	12	117	theme	functional	2213:2222	arg1	scaffolds					2235:2243	functional wound care scaffolds	2213:2243	functional wound care scaffolds as well as its diverse potential	2213:2276	Our initial observations revealed the credibility of these sponges as functional wound care scaffolds as well as its diverse potential as a suitable substrate for various tissue engineering applications.
33254970	12	117	theme	functional	2213:2222	arg1	credibility					2181:2191	the credibility	2177:2191	the credibility of these sponges	2177:2208	Our initial observations revealed the credibility of these sponges as functional wound care scaffolds as well as its diverse potential as a suitable substrate for various tissue engineering applications.
33254970	2	118	from	bio-extracts	402:413	arg1	amalgamation					348:359	the aqueous amalgamation	336:359	the aqueous amalgamation of SA and cellulose nanofibres (CNFs) in bio-extracts like Rice water (Rw) and Giloy extract (Ge)	336:457	The composite bio-sponges were developed from the aqueous amalgamation of SA and cellulose nanofibres (CNFs) in bio-extracts like Rice water (Rw) and Giloy extract (Ge).
33254970	4	119	with	Bio-sponges	703:713	arg1	structures					762:771	shared interpenetrating polymeric network structures	720:771	shared interpenetrating polymeric network structures	720:771	Bio-sponges with shared interpenetrating polymeric network structures were attained at optimized BioPU coating formulation.
33254970	8	120	theme	structural	1508:1517	arg1	stability					1519:1527	their structural stability	1502:1527	their structural stability	1502:1527	The physical entanglement of BioPU chains with core structural components of sponge improved their structural stability by suppressing their full fragmentation in water-based medium without affecting its swelling behaviour (swelling ratio > 1000%).
33254970	2	121	theme	cellulose	371:379	arg1	CNFs					393:396	CNFs	393:396	CNFs	393:396	The composite bio-sponges were developed from the aqueous amalgamation of SA and cellulose nanofibres (CNFs) in bio-extracts like Rice water (Rw) and Giloy extract (Ge).
33254970	2	121	theme	cellulose	371:379	arg1	nanofibres					381:390	SA and cellulose nanofibres	364:390	nanofibres	381:390	The composite bio-sponges were developed from the aqueous amalgamation of SA and cellulose nanofibres (CNFs) in bio-extracts like Rice water (Rw) and Giloy extract (Ge).
33254970	1	122	theme	potential	244:252	arg1	application					254:264	potential application	244:264	potential application as wound care scaffold	244:287	In this work, sodium alginate (SA) based "all-natural" composite bio-sponges were designed for potential application as wound care scaffold.
32804173	5	0	theme	labeled	724:730	arg1	glycans					732:738	native and labeled glycans	713:738	native and labeled glycans	713:738	GUI has previously been utilized for retention time normalization of native and labeled glycans.
32804173	10	1	theme	134	1464:1466	arg1	glycans					1468:1474	134 glycans	1464:1474	134 glycans	1464:1474	A total of 116 permethylated N-glycans separated on a C18 column and 134 glycans separated on a PGC column were compiled in a library.
32804173	6	2	theme	ribonuclease	849:860	arg1	glycoproteins					824:836	model glycoproteins fetuin and ribonuclease B (RNase B)	818:872	model glycoproteins fetuin and ribonuclease B (RNase B)	818:872	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	6	2	theme	ribonuclease	849:860	arg1	B					862:862	ribonuclease B	849:862	ribonuclease B (RNase B)	849:872	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	6	2	theme	ribonuclease	849:860	arg1	B					871:871	RNase B	865:871	RNase B	865:871	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	8	3	theme	UltiMate	1195:1202	arg1	systems					1186:1192	three different LC systems	1167:1192	three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories	1167:1247	The GUI values were validated on three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories to ensure the reliability and reproducibility of the method.
32804173	8	3	theme	UltiMate	1195:1202	arg1	systems					1220:1226	UltiMate 3000 Nano UHPLC systems	1195:1226	UltiMate 3000 Nano UHPLC systems	1195:1226	The GUI values were validated on three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories to ensure the reliability and reproducibility of the method.
32804173	7	4	attach	derived	1069:1075	arg1	standard					1124:1131	standard	1124:1131	standard	1124:1131	GUI values for glycan compositions were calculated with respect to the glucose units derived from dextrin, which was employed as an elution standard.
32804173	7	4	attach	derived	1069:1075	arg2	units					1063:1067	the glucose units	1051:1067	the glucose units derived from dextrin, which was employed as an elution standard	1051:1131	GUI values for glycan compositions were calculated with respect to the glucose units derived from dextrin, which was employed as an elution standard.
32804173	7	4	attach	derived	1069:1075	arg1	dextrin					1082:1088	dextrin	1082:1088	dextrin	1082:1088	GUI values for glycan compositions were calculated with respect to the glucose units derived from dextrin, which was employed as an elution standard.
32804173	4	5	theme	Unit	550:553	arg1	Index					555:559	the Glucose Unit Index	538:559	the Glucose Unit Index (GUI)	538:565	In our endeavor to overcome this variance, we propose the use of the Glucose Unit Index (GUI) on C18 and PGC column-based separation of reduced and permethylated glycans.
32804173	4	5	theme	Unit	550:553	arg1	GUI					562:564	GUI	562:564	GUI	562:564	In our endeavor to overcome this variance, we propose the use of the Glucose Unit Index (GUI) on C18 and PGC column-based separation of reduced and permethylated glycans.
32804173	0	6	theme	effective	54:62	arg1	identification					64:77	effective identification	54:77	effective identification of glycans and glycan isomers	54:107	Glucose unit index (GUI) of permethylated glycans for effective identification of glycans and glycan isomers.
32804173	11	7	theme	glycan	1709:1714	arg1	methods					1731:1737	automated glycan and isomeric glycan identification methods	1679:1737	methods	1731:1737	Overall, the established GUI method and the demonstration of reproducible inter- and intra-laboratory GUI values would aid the future development of automated glycan and isomeric glycan identification methods.
32804173	1	8	theme	common	137:142	arg1	criterion					160:168	the most common and widely used criterion	128:168	the most common and widely used criterion to report the separation of glycans using Liquid Chromatography (LC)	128:237	Retention time is the most common and widely used criterion to report the separation of glycans using Liquid Chromatography (LC), but it varies widely across different columns, instruments and laboratories.
32804173	1	8	theme	common	137:142	arg1	time					120:123	Retention time	110:123	Retention time	110:123	Retention time is the most common and widely used criterion to report the separation of glycans using Liquid Chromatography (LC), but it varies widely across different columns, instruments and laboratories.
32804173	10	9	theme	permethylated	1410:1422	arg1	N-glycans					1424:1432	116 permethylated N-glycans	1406:1432	116 permethylated N-glycans separated on a C18 column and 134 glycans separated on a PGC column	1406:1500	A total of 116 permethylated N-glycans separated on a C18 column and 134 glycans separated on a PGC column were compiled in a library.
32804173	4	10	theme	Glucose	542:548	arg1	Index					555:559	the Glucose Unit Index	538:559	the Glucose Unit Index (GUI)	538:565	In our endeavor to overcome this variance, we propose the use of the Glucose Unit Index (GUI) on C18 and PGC column-based separation of reduced and permethylated glycans.
32804173	4	10	theme	Glucose	542:548	arg1	GUI					562:564	GUI	562:564	GUI	562:564	In our endeavor to overcome this variance, we propose the use of the Glucose Unit Index (GUI) on C18 and PGC column-based separation of reduced and permethylated glycans.
32804173	0	11	theme	glycans	82:88	arg1	identification					64:77	effective identification	54:77	effective identification of glycans and glycan isomers	54:107	Glucose unit index (GUI) of permethylated glycans for effective identification of glycans and glycan isomers.
32804173	11	12	theme	established	1543:1553	arg1	method					1559:1564	the established GUI method	1539:1564	the established GUI method	1539:1564	Overall, the established GUI method and the demonstration of reproducible inter- and intra-laboratory GUI values would aid the future development of automated glycan and isomeric glycan identification methods.
32804173	7	13	theme	glucose	1055:1061	arg1	units					1063:1067	the glucose units	1051:1067	the glucose units derived from dextrin, which was employed as an elution standard	1051:1131	GUI values for glycan compositions were calculated with respect to the glucose units derived from dextrin, which was employed as an elution standard.
32804173	11	14	theme	automated	1679:1687	arg1	glycan					1689:1694	automated glycan and isomeric glycan identification methods	1679:1737	glycan	1689:1694	Overall, the established GUI method and the demonstration of reproducible inter- and intra-laboratory GUI values would aid the future development of automated glycan and isomeric glycan identification methods.
32804173	5	15	theme	time	691:694	arg1	normalization					696:708	retention time normalization	681:708	retention time normalization of native and labeled glycans	681:738	GUI has previously been utilized for retention time normalization of native and labeled glycans.
32804173	6	16	theme	RNase	865:869	arg1	B					862:862	ribonuclease B	849:862	ribonuclease B (RNase B)	849:872	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	6	16	theme	RNase	865:869	arg1	B					871:871	RNase B	865:871	RNase B	865:871	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	10	17	theme	N-glycans	1424:1432	arg1	total					1397:1401	A total	1395:1401	A total of 116 permethylated N-glycans separated on a C18 column and 134 glycans separated on a PGC column	1395:1500	A total of 116 permethylated N-glycans separated on a C18 column and 134 glycans separated on a PGC column were compiled in a library.
32804173	4	18	theme	Index	555:559	arg1	use					531:533	the use	527:533	the use of the Glucose Unit Index (GUI) on C18 and PGC column-based separation of reduced and permethylated glycans	527:641	In our endeavor to overcome this variance, we propose the use of the Glucose Unit Index (GUI) on C18 and PGC column-based separation of reduced and permethylated glycans.
32804173	0	19	theme	glycan	94:99	arg1	isomers					101:107	glycan isomers	94:107	glycan isomers	94:107	Glucose unit index (GUI) of permethylated glycans for effective identification of glycans and glycan isomers.
32804173	9	20	theme	real	1327:1330	arg1	samples					1332:1338	real samples	1327:1338	real samples	1327:1338	Applicability on real samples was demonstrated using human breast cancer cell lines.
32804173	4	21	theme	permethylated	621:633	arg1	glycans					635:641	permethylated glycans	621:641	permethylated glycans	621:641	In our endeavor to overcome this variance, we propose the use of the Glucose Unit Index (GUI) on C18 and PGC column-based separation of reduced and permethylated glycans.
32804173	4	22	theme	reduced	609:615	arg1	separation					595:604	PGC column-based separation	578:604	PGC column-based separation	578:604	In our endeavor to overcome this variance, we propose the use of the Glucose Unit Index (GUI) on C18 and PGC column-based separation of reduced and permethylated glycans.
32804173	4	22	theme	reduced	609:615	arg1	C18					570:572	C18	570:572	C18	570:572	In our endeavor to overcome this variance, we propose the use of the Glucose Unit Index (GUI) on C18 and PGC column-based separation of reduced and permethylated glycans.
32804173	9	23	theme	human	1363:1367	arg1	lines					1388:1392	human breast cancer cell lines	1363:1392	human breast cancer cell lines	1363:1392	Applicability on real samples was demonstrated using human breast cancer cell lines.
32804173	11	24	theme	future	1657:1662	arg1	development					1664:1674	the future development	1653:1674	the future development of automated glycan and isomeric glycan identification methods	1653:1737	Overall, the established GUI method and the demonstration of reproducible inter- and intra-laboratory GUI values would aid the future development of automated glycan and isomeric glycan identification methods.
32804173	6	25	theme	glycan	966:971	arg1	libraries					973:981	column-based isomeric glycan libraries	944:981	column-based isomeric glycan libraries	944:981	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	2	26	theme	inter-laboratory	352:367	arg1	data					369:372	inter-laboratory data	352:372	inter-laboratory data	352:372	This variation is problematic when inter-laboratory data is compared.
32804173	0	27	theme	unit	8:11	arg1	index					13:17	Glucose unit index	0:17	Glucose unit index (GUI) of permethylated glycans for effective identification of glycans and glycan isomers	0:107	Glucose unit index (GUI) of permethylated glycans for effective identification of glycans and glycan isomers.
32804173	0	27	theme	unit	8:11	arg1	GUI					20:22	GUI	20:22	GUI	20:22	Glucose unit index (GUI) of permethylated glycans for effective identification of glycans and glycan isomers.
32804173	5	28	theme	glycans	732:738	arg1	normalization					696:708	retention time normalization	681:708	retention time normalization of native and labeled glycans	681:738	GUI has previously been utilized for retention time normalization of native and labeled glycans.
32804173	10	29	theme	PGC	1491:1493	arg1	column					1495:1500	a PGC column	1489:1500	a PGC column	1489:1500	A total of 116 permethylated N-glycans separated on a C18 column and 134 glycans separated on a PGC column were compiled in a library.
32804173	1	30	theme	used	155:158	arg1	criterion					160:168	the most common and widely used criterion	128:168	the most common and widely used criterion to report the separation of glycans using Liquid Chromatography (LC)	128:237	Retention time is the most common and widely used criterion to report the separation of glycans using Liquid Chromatography (LC), but it varies widely across different columns, instruments and laboratories.
32804173	1	30	theme	used	155:158	arg1	time					120:123	Retention time	110:123	Retention time	110:123	Retention time is the most common and widely used criterion to report the separation of glycans using Liquid Chromatography (LC), but it varies widely across different columns, instruments and laboratories.
32804173	0	31	theme	Glucose	0:6	arg1	index					13:17	Glucose unit index	0:17	Glucose unit index (GUI) of permethylated glycans for effective identification of glycans and glycan isomers	0:107	Glucose unit index (GUI) of permethylated glycans for effective identification of glycans and glycan isomers.
32804173	0	31	theme	Glucose	0:6	arg1	GUI					20:22	GUI	20:22	GUI	20:22	Glucose unit index (GUI) of permethylated glycans for effective identification of glycans and glycan isomers.
32804173	11	32	theme	methods	1731:1737	arg1	development					1664:1674	the future development	1653:1674	the future development of automated glycan and isomeric glycan identification methods	1653:1737	Overall, the established GUI method and the demonstration of reproducible inter- and intra-laboratory GUI values would aid the future development of automated glycan and isomeric glycan identification methods.
32804173	8	33	theme	different	1173:1181	arg1	systems					1186:1192	three different LC systems	1167:1192	three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories	1167:1247	The GUI values were validated on three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories to ensure the reliability and reproducibility of the method.
32804173	8	33	theme	different	1173:1181	arg1	systems					1220:1226	UltiMate 3000 Nano UHPLC systems	1195:1226	UltiMate 3000 Nano UHPLC systems	1195:1226	The GUI values were validated on three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories to ensure the reliability and reproducibility of the method.
32804173	8	34	theme	LC	1183:1184	arg1	systems					1186:1192	three different LC systems	1167:1192	three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories	1167:1247	The GUI values were validated on three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories to ensure the reliability and reproducibility of the method.
32804173	8	34	theme	LC	1183:1184	arg1	systems					1220:1226	UltiMate 3000 Nano UHPLC systems	1195:1226	UltiMate 3000 Nano UHPLC systems	1195:1226	The GUI values were validated on three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories to ensure the reliability and reproducibility of the method.
32804173	4	35	from	use	531:533	arg1	separation					595:604	PGC column-based separation	578:604	PGC column-based separation	578:604	In our endeavor to overcome this variance, we propose the use of the Glucose Unit Index (GUI) on C18 and PGC column-based separation of reduced and permethylated glycans.
32804173	4	35	from	use	531:533	arg1	C18					570:572	C18	570:572	C18	570:572	In our endeavor to overcome this variance, we propose the use of the Glucose Unit Index (GUI) on C18 and PGC column-based separation of reduced and permethylated glycans.
32804173	9	36	theme	breast	1369:1374	arg1	lines					1388:1392	human breast cancer cell lines	1363:1392	human breast cancer cell lines	1363:1392	Applicability on real samples was demonstrated using human breast cancer cell lines.
32804173	11	37	theme	glycan	1689:1694	arg1	development					1664:1674	the future development	1653:1674	the future development of automated glycan and isomeric glycan identification methods	1653:1737	Overall, the established GUI method and the demonstration of reproducible inter- and intra-laboratory GUI values would aid the future development of automated glycan and isomeric glycan identification methods.
32804173	4	38	theme	PGC	578:580	arg1	separation					595:604	PGC column-based separation	578:604	PGC column-based separation	578:604	In our endeavor to overcome this variance, we propose the use of the Glucose Unit Index (GUI) on C18 and PGC column-based separation of reduced and permethylated glycans.
32804173	6	39	theme	blood	908:912	arg1	serum					914:918	human blood serum	902:918	human blood serum	902:918	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	0	40	theme	isomers	101:107	arg1	identification					64:77	effective identification	54:77	effective identification of glycans and glycan isomers	54:107	Glucose unit index (GUI) of permethylated glycans for effective identification of glycans and glycan isomers.
32804173	8	41	theme	method	1302:1307	arg1	reproducibility					1279:1293	reproducibility	1279:1293	reproducibility	1279:1293	The GUI values were validated on three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories to ensure the reliability and reproducibility of the method.
32804173	8	41	theme	method	1302:1307	arg1	reliability					1263:1273	reliability	1263:1273	reliability	1263:1273	The GUI values were validated on three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories to ensure the reliability and reproducibility of the method.
32804173	3	42	theme	data	452:455	arg1	interpretation					457:470	efficient data interpretation	442:470	efficient data interpretation	442:470	Furthermore, it influences reproducibility and hampers efficient data interpretation.
32804173	6	43	theme	human	902:906	arg1	serum					914:918	human blood serum	902:918	human blood serum	902:918	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	7	44	theme	glycan	999:1004	arg1	compositions					1006:1017	glycan compositions	999:1017	glycan compositions	999:1017	GUI values for glycan compositions were calculated with respect to the glucose units derived from dextrin, which was employed as an elution standard.
32804173	8	45	dep	reliability	1263:1273	arg1	the					1259:1261	the	1259:1261	the	1259:1261	The GUI values were validated on three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories to ensure the reliability and reproducibility of the method.
32804173	11	46	theme	intra-laboratory	1615:1630	arg1	values					1636:1641	reproducible inter- and intra-laboratory GUI values	1591:1641	reproducible inter- and intra-laboratory GUI values	1591:1641	Overall, the established GUI method and the demonstration of reproducible inter- and intra-laboratory GUI values would aid the future development of automated glycan and isomeric glycan identification methods.
32804173	6	47	gly	glycoproteins	824:836	arg1	glycoproteins					824:836	model glycoproteins fetuin and ribonuclease B (RNase B)	818:872	model glycoproteins fetuin and ribonuclease B (RNase B)	818:872	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	6	47	gly	glycoproteins	824:836	arg1	fetuin					838:843	fetuin	838:843	fetuin	838:843	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	6	47	gly	glycoproteins	824:836	arg1	B					862:862	ribonuclease B	849:862	ribonuclease B (RNase B)	849:872	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	8	48	theme	Nano	1209:1212	arg1	systems					1186:1192	three different LC systems	1167:1192	three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories	1167:1247	The GUI values were validated on three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories to ensure the reliability and reproducibility of the method.
32804173	8	48	theme	Nano	1209:1212	arg1	systems					1220:1226	UltiMate 3000 Nano UHPLC systems	1195:1226	UltiMate 3000 Nano UHPLC systems	1195:1226	The GUI values were validated on three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories to ensure the reliability and reproducibility of the method.
32804173	5	49	used	utilized	668:675	arg2	GUI					644:646	GUI	644:646	GUI	644:646	GUI has previously been utilized for retention time normalization of native and labeled glycans.
32804173	6	50	dep	glycoproteins	824:836	arg1	glycoproteins					824:836	model glycoproteins fetuin and ribonuclease B (RNase B)	818:872	model glycoproteins fetuin and ribonuclease B (RNase B)	818:872	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	6	50	dep	glycoproteins	824:836	arg1	fetuin					838:843	fetuin	838:843	fetuin	838:843	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	6	50	dep	glycoproteins	824:836	arg1	B					862:862	ribonuclease B	849:862	ribonuclease B (RNase B)	849:872	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	6	50	dep	glycoproteins	824:836	arg1	B					871:871	RNase B	865:871	RNase B	865:871	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	11	51	theme	isomeric	1700:1707	arg1	methods					1731:1737	automated glycan and isomeric glycan identification methods	1679:1737	methods	1731:1737	Overall, the established GUI method and the demonstration of reproducible inter- and intra-laboratory GUI values would aid the future development of automated glycan and isomeric glycan identification methods.
32804173	11	52	theme	reproducible	1591:1602	arg1	values					1636:1641	reproducible inter- and intra-laboratory GUI values	1591:1641	reproducible inter- and intra-laboratory GUI values	1591:1641	Overall, the established GUI method and the demonstration of reproducible inter- and intra-laboratory GUI values would aid the future development of automated glycan and isomeric glycan identification methods.
32804173	8	53	theme	GUI	1138:1140	arg1	values					1142:1147	The GUI values	1134:1147	The GUI values	1134:1147	The GUI values were validated on three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories to ensure the reliability and reproducibility of the method.
32804173	8	54	from	systems	1186:1192	arg1	laboratories					1236:1247	two laboratories	1232:1247	two laboratories	1232:1247	The GUI values were validated on three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories to ensure the reliability and reproducibility of the method.
32804173	4	55	theme	column-based	582:593	arg1	separation					595:604	PGC column-based separation	578:604	PGC column-based separation	578:604	In our endeavor to overcome this variance, we propose the use of the Glucose Unit Index (GUI) on C18 and PGC column-based separation of reduced and permethylated glycans.
32804173	0	56	theme	permethylated	28:40	arg1	glycans					42:48	permethylated glycans	28:48	permethylated glycans	28:48	Glucose unit index (GUI) of permethylated glycans for effective identification of glycans and glycan isomers.
32804173	5	57	theme	retention	681:689	arg1	normalization					696:708	retention time normalization	681:708	retention time normalization of native and labeled glycans	681:738	GUI has previously been utilized for retention time normalization of native and labeled glycans.
32804173	6	58	theme	model	818:822	arg1	glycoproteins					824:836	model glycoproteins fetuin and ribonuclease B (RNase B)	818:872	model glycoproteins fetuin and ribonuclease B (RNase B)	818:872	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	6	58	theme	model	818:822	arg1	fetuin					838:843	fetuin	838:843	fetuin	838:843	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	6	58	theme	model	818:822	arg1	B					862:862	ribonuclease B	849:862	ribonuclease B (RNase B)	849:872	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	6	59	attach	derived	805:811	arg1	glycoproteins					824:836	model glycoproteins fetuin and ribonuclease B (RNase B)	818:872	model glycoproteins fetuin and ribonuclease B (RNase B)	818:872	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	6	59	attach	derived	805:811	arg2	reduced					771:777	reduced	771:777	reduced	771:777	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	6	59	attach	derived	805:811	arg1	fetuin					838:843	fetuin	838:843	fetuin	838:843	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	6	59	attach	derived	805:811	arg1	B					862:862	ribonuclease B	849:862	ribonuclease B (RNase B)	849:872	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	11	60	theme	GUI	1555:1557	arg1	method					1559:1564	the established GUI method	1539:1564	the established GUI method	1539:1564	Overall, the established GUI method and the demonstration of reproducible inter- and intra-laboratory GUI values would aid the future development of automated glycan and isomeric glycan identification methods.
32804173	1	61	theme	different	268:276	arg1	columns					278:284	different columns	268:284	different columns	268:284	Retention time is the most common and widely used criterion to report the separation of glycans using Liquid Chromatography (LC), but it varies widely across different columns, instruments and laboratories.
32804173	10	62	theme	C18	1449:1451	arg1	column					1453:1458	a C18 column	1447:1458	a C18 column	1447:1458	A total of 116 permethylated N-glycans separated on a C18 column and 134 glycans separated on a PGC column were compiled in a library.
32804173	8	63	theme	UHPLC	1214:1218	arg1	systems					1186:1192	three different LC systems	1167:1192	three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories	1167:1247	The GUI values were validated on three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories to ensure the reliability and reproducibility of the method.
32804173	8	63	theme	UHPLC	1214:1218	arg1	systems					1220:1226	UltiMate 3000 Nano UHPLC systems	1195:1226	UltiMate 3000 Nano UHPLC systems	1195:1226	The GUI values were validated on three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories to ensure the reliability and reproducibility of the method.
32804173	9	64	from	Applicability	1310:1322	arg1	samples					1332:1338	real samples	1327:1338	real samples	1327:1338	Applicability on real samples was demonstrated using human breast cancer cell lines.
32804173	11	65	theme	identification	1716:1729	arg1	methods					1731:1737	automated glycan and isomeric glycan identification methods	1679:1737	methods	1731:1737	Overall, the established GUI method and the demonstration of reproducible inter- and intra-laboratory GUI values would aid the future development of automated glycan and isomeric glycan identification methods.
32804173	11	66	theme	values	1636:1641	arg1	method					1559:1564	the established GUI method	1539:1564	the established GUI method	1539:1564	Overall, the established GUI method and the demonstration of reproducible inter- and intra-laboratory GUI values would aid the future development of automated glycan and isomeric glycan identification methods.
32804173	11	66	theme	values	1636:1641	arg1	demonstration					1574:1586	the demonstration	1570:1586	the demonstration of reproducible inter- and intra-laboratory GUI values	1570:1641	Overall, the established GUI method and the demonstration of reproducible inter- and intra-laboratory GUI values would aid the future development of automated glycan and isomeric glycan identification methods.
32804173	1	67	theme	Retention	110:118	arg1	time					120:123	Retention time	110:123	Retention time	110:123	Retention time is the most common and widely used criterion to report the separation of glycans using Liquid Chromatography (LC), but it varies widely across different columns, instruments and laboratories.
32804173	1	67	theme	Retention	110:118	arg1	criterion					160:168	the most common and widely used criterion	128:168	the most common and widely used criterion to report the separation of glycans using Liquid Chromatography (LC)	128:237	Retention time is the most common and widely used criterion to report the separation of glycans using Liquid Chromatography (LC), but it varies widely across different columns, instruments and laboratories.
32804173	8	68	theme	3000	1204:1207	arg1	systems					1186:1192	three different LC systems	1167:1192	three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories	1167:1247	The GUI values were validated on three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories to ensure the reliability and reproducibility of the method.
32804173	8	68	theme	3000	1204:1207	arg1	systems					1220:1226	UltiMate 3000 Nano UHPLC systems	1195:1226	UltiMate 3000 Nano UHPLC systems	1195:1226	The GUI values were validated on three different LC systems (UltiMate 3000 Nano UHPLC systems) in two laboratories to ensure the reliability and reproducibility of the method.
32804173	7	69	theme	GUI	984:986	arg1	values					988:993	GUI values	984:993	GUI values for glycan compositions	984:1017	GUI values for glycan compositions were calculated with respect to the glucose units derived from dextrin, which was employed as an elution standard.
32804173	11	70	theme	GUI	1632:1634	arg1	values					1636:1641	reproducible inter- and intra-laboratory GUI values	1591:1641	reproducible inter- and intra-laboratory GUI values	1591:1641	Overall, the established GUI method and the demonstration of reproducible inter- and intra-laboratory GUI values would aid the future development of automated glycan and isomeric glycan identification methods.
32804173	9	71	theme	cell	1383:1386	arg1	lines					1388:1392	human breast cancer cell lines	1363:1392	human breast cancer cell lines	1363:1392	Applicability on real samples was demonstrated using human breast cancer cell lines.
32804173	1	72	theme	glycans	198:204	arg1	separation					184:193	the separation	180:193	the separation of glycans	180:204	Retention time is the most common and widely used criterion to report the separation of glycans using Liquid Chromatography (LC), but it varies widely across different columns, instruments and laboratories.
32804173	5	73	theme	native	713:718	arg1	glycans					732:738	native and labeled glycans	713:738	native and labeled glycans	713:738	GUI has previously been utilized for retention time normalization of native and labeled glycans.
32804173	6	74	theme	permethylated	783:795	arg1	glycans					797:803	permethylated glycans	783:803	permethylated glycans	783:803	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	3	75	theme	efficient	442:450	arg1	interpretation					457:470	efficient data interpretation	442:470	efficient data interpretation	442:470	Furthermore, it influences reproducibility and hampers efficient data interpretation.
32804173	9	76	theme	cancer	1376:1381	arg1	lines					1388:1392	human breast cancer cell lines	1363:1392	human breast cancer cell lines	1363:1392	Applicability on real samples was demonstrated using human breast cancer cell lines.
32804173	6	77	theme	isomeric	957:964	arg1	libraries					973:981	column-based isomeric glycan libraries	944:981	column-based isomeric glycan libraries	944:981	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	0	78	theme	glycans	42:48	arg1	index					13:17	Glucose unit index	0:17	Glucose unit index (GUI) of permethylated glycans for effective identification of glycans and glycan isomers	0:107	Glucose unit index (GUI) of permethylated glycans for effective identification of glycans and glycan isomers.
32804173	0	78	theme	glycans	42:48	arg1	GUI					20:22	GUI	20:22	GUI	20:22	Glucose unit index (GUI) of permethylated glycans for effective identification of glycans and glycan isomers.
32804173	6	79	dep	C18	932:934	arg1	libraries					973:981	column-based isomeric glycan libraries	944:981	column-based isomeric glycan libraries	944:981	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	6	80	theme	column-based	944:955	arg1	libraries					973:981	column-based isomeric glycan libraries	944:981	column-based isomeric glycan libraries	944:981	We evaluated this method with reduced and permethylated glycans derived from model glycoproteins fetuin and ribonuclease B (RNase B), and then implemented it to human blood serum to generate C18 and PGC column-based isomeric glycan libraries.
32804173	4	81	theme	glycans	635:641	arg1	separation					595:604	PGC column-based separation	578:604	PGC column-based separation	578:604	In our endeavor to overcome this variance, we propose the use of the Glucose Unit Index (GUI) on C18 and PGC column-based separation of reduced and permethylated glycans.
32804173	4	81	theme	glycans	635:641	arg1	C18					570:572	C18	570:572	C18	570:572	In our endeavor to overcome this variance, we propose the use of the Glucose Unit Index (GUI) on C18 and PGC column-based separation of reduced and permethylated glycans.
32804173	11	82	theme	inter-	1604:1609	arg1	values					1636:1641	reproducible inter- and intra-laboratory GUI values	1591:1641	reproducible inter- and intra-laboratory GUI values	1591:1641	Overall, the established GUI method and the demonstration of reproducible inter- and intra-laboratory GUI values would aid the future development of automated glycan and isomeric glycan identification methods.
32804173	1	83	theme	Liquid	212:217	arg1	LC					235:236	LC	235:236	LC	235:236	Retention time is the most common and widely used criterion to report the separation of glycans using Liquid Chromatography (LC), but it varies widely across different columns, instruments and laboratories.
32804173	1	83	theme	Liquid	212:217	arg1	Chromatography					219:232	Liquid Chromatography	212:232	Liquid Chromatography (LC)	212:237	Retention time is the most common and widely used criterion to report the separation of glycans using Liquid Chromatography (LC), but it varies widely across different columns, instruments and laboratories.
31954311	9	0	theme	first	1241:1245	arg1	report					1247:1252	the first report	1237:1252	the first report showing exceptional Th-tolerance by any bacteria	1237:1301	To the best of our knowledge, this is the first report showing exceptional Th-tolerance by any bacteria.
31954311	9	0	theme	first	1241:1245	arg1	this					1229:1232	this	1229:1232	this	1229:1232	To the best of our knowledge, this is the first report showing exceptional Th-tolerance by any bacteria.
31954311	8	1	theme	FTIR	1115:1118	arg1	spectroscopy					1120:1131	FTIR spectroscopy	1115:1131	FTIR spectroscopy	1115:1131	FTIR spectroscopy revealed the functional groups of EPS involved in EPS-Th binding.
31954311	10	2	theme	environment-friendly	1360:1379	arg1	way					1381:1383	an environment-friendly way	1357:1383	an environment-friendly way of radwaste disposal	1357:1404	Such study will help other researchers to strategize an environment-friendly way of radwaste disposal.
31954311	4	3	theme	O.	562:563	arg1	intermedium					565:575	O. intermedium	562:575	O. intermedium	562:575	Of all the isolates, strain AM7 identified as O. intermedium was selected since it could thrive at high levels of Th (1000 mg L-1).
31954311	0	4	theme	EPS-Thorium	102:112	arg1	binding					114:120	its EPS-Thorium binding	98:120	its EPS-Thorium binding	98:120	Characterization of novel thorium tolerant Ochrobactrum intermedium AM7 in consort with assessing its EPS-Thorium binding.
31954311	10	5	theme	other	1325:1329	arg1	researchers					1331:1341	other researchers	1325:1341	other researchers	1325:1341	Such study will help other researchers to strategize an environment-friendly way of radwaste disposal.
31954311	9	6	theme	exceptional	1262:1272	arg1	Th-tolerance					1274:1285	exceptional Th-tolerance	1262:1285	exceptional Th-tolerance	1262:1285	To the best of our knowledge, this is the first report showing exceptional Th-tolerance by any bacteria.
31954311	6	7	theme	exopolysaccharide	879:895	arg1	production					903:912	exceptional exopolysaccharide (EPS) production	867:912	exceptional exopolysaccharide (EPS) production	867:912	The strain also showed exceptional exopolysaccharide (EPS) production and its yield was further analyzed using one factor study to investigate the influence of each medium component.
31954311	8	8	theme	functional	1146:1155	arg1	EPS					1167:1169	EPS	1167:1169	EPS involved in EPS-Th binding	1167:1196	FTIR spectroscopy revealed the functional groups of EPS involved in EPS-Th binding.
31954311	8	8	theme	functional	1146:1155	arg1	groups					1157:1162	the functional groups	1142:1162	the functional groups of EPS involved in EPS-Th binding	1142:1196	FTIR spectroscopy revealed the functional groups of EPS involved in EPS-Th binding.
31954311	6	9	theme	exceptional	867:877	arg1	exopolysaccharide					879:895	exceptional exopolysaccharide	867:895	exceptional exopolysaccharide (EPS) production	867:912	The strain also showed exceptional exopolysaccharide (EPS) production and its yield was further analyzed using one factor study to investigate the influence of each medium component.
31954311	6	9	theme	exceptional	867:877	arg1	EPS					898:900	EPS	898:900	EPS	898:900	The strain also showed exceptional exopolysaccharide (EPS) production and its yield was further analyzed using one factor study to investigate the influence of each medium component.
31954311	0	10	theme	Ochrobactrum	43:54	arg1	intermedium					56:66	novel thorium tolerant Ochrobactrum intermedium	20:66	novel thorium tolerant Ochrobactrum intermedium	20:66	Characterization of novel thorium tolerant Ochrobactrum intermedium AM7 in consort with assessing its EPS-Thorium binding.
31954311	10	11	theme	disposal	1397:1404	arg1	way					1381:1383	an environment-friendly way	1357:1383	an environment-friendly way of radwaste disposal	1357:1404	Such study will help other researchers to strategize an environment-friendly way of radwaste disposal.
31954311	4	12	theme	Th	630:631	arg1	levels					620:625	high levels	615:625	high levels of Th (1000 mg L-1)	615:645	Of all the isolates, strain AM7 identified as O. intermedium was selected since it could thrive at high levels of Th (1000 mg L-1).
31954311	3	13	theme	atomic	396:401	arg1	station					409:415	atomic power station	396:415	atomic power station exposed to radionuclides and heavy metals	396:457	In the present study the bacterial flora, from soil sample collected from an area around atomic power station exposed to radionuclides and heavy metals, was cultivated and assessed for thorium (Th) tolerance.
31954311	10	14	theme	radwaste	1388:1395	arg1	disposal					1397:1404	radwaste disposal	1388:1404	radwaste disposal	1388:1404	Such study will help other researchers to strategize an environment-friendly way of radwaste disposal.
31954311	3	15	theme	power	403:407	arg1	station					409:415	atomic power station	396:415	atomic power station exposed to radionuclides and heavy metals	396:457	In the present study the bacterial flora, from soil sample collected from an area around atomic power station exposed to radionuclides and heavy metals, was cultivated and assessed for thorium (Th) tolerance.
31954311	6	16	theme	factor	959:964	arg1	study					966:970	one factor study	955:970	one factor study	955:970	The strain also showed exceptional exopolysaccharide (EPS) production and its yield was further analyzed using one factor study to investigate the influence of each medium component.
31954311	3	17	theme	heavy	446:450	arg1	metals					452:457	heavy metals	446:457	heavy metals	446:457	In the present study the bacterial flora, from soil sample collected from an area around atomic power station exposed to radionuclides and heavy metals, was cultivated and assessed for thorium (Th) tolerance.
31954311	5	18	theme	growth	807:812	arg1	properties					814:823	its growth properties	803:823	its growth properties	803:823	AM7 was characterized physico-chemically and its culture medium was optimized using central composite design of response surface methodology for assessing its growth properties in presence of Th.
31954311	4	19	theme	high	615:618	arg1	levels					620:625	high levels	615:625	high levels of Th (1000 mg L-1)	615:645	Of all the isolates, strain AM7 identified as O. intermedium was selected since it could thrive at high levels of Th (1000 mg L-1).
31954311	0	20	theme	novel	20:24	arg1	intermedium					56:66	novel thorium tolerant Ochrobactrum intermedium	20:66	novel thorium tolerant Ochrobactrum intermedium	20:66	Characterization of novel thorium tolerant Ochrobactrum intermedium AM7 in consort with assessing its EPS-Thorium binding.
31954311	3	21	from	sample	359:364	arg1	flora					342:346	the bacterial flora	328:346	the bacterial flora	328:346	In the present study the bacterial flora, from soil sample collected from an area around atomic power station exposed to radionuclides and heavy metals, was cultivated and assessed for thorium (Th) tolerance.
31954311	5	22	theme	response	760:767	arg1	methodology					777:787	response surface methodology	760:787	response surface methodology for assessing its growth properties in presence of Th	760:841	AM7 was characterized physico-chemically and its culture medium was optimized using central composite design of response surface methodology for assessing its growth properties in presence of Th.
31954311	2	23	theme	contaminated	249:260	arg1	environment					262:272	such contaminated environment	244:272	such contaminated environment	244:272	Microorganisms thriving in such contaminated environment show tolerance to radionuclides.
31954311	1	24	theme	deep	189:192	arg1	repository					205:214	a deep geological repository	187:214	a deep geological repository	187:214	Currently, radioactive waste is disposed primarily by burial in a deep geological repository.
31954311	5	25	theme	surface	769:775	arg1	methodology					777:787	response surface methodology	760:787	response surface methodology for assessing its growth properties in presence of Th	760:841	AM7 was characterized physico-chemically and its culture medium was optimized using central composite design of response surface methodology for assessing its growth properties in presence of Th.
31954311	7	26	from	decrease	1083:1090	arg1	yield					1095:1099	yield	1095:1099	yield	1095:1099	On supplementing the EPS medium with Th, no significant decrease in yield was observed.
31954311	5	27	theme	culture	697:703	arg1	medium					705:710	its culture medium	693:710	its culture medium	693:710	AM7 was characterized physico-chemically and its culture medium was optimized using central composite design of response surface methodology for assessing its growth properties in presence of Th.
31954311	2	28	theme	such	244:247	arg1	environment					262:272	such contaminated environment	244:272	such contaminated environment	244:272	Microorganisms thriving in such contaminated environment show tolerance to radionuclides.
31954311	5	29	theme	Th	840:841	arg1	presence					828:835	presence	828:835	presence of Th	828:841	AM7 was characterized physico-chemically and its culture medium was optimized using central composite design of response surface methodology for assessing its growth properties in presence of Th.
31954311	5	30	theme	methodology	777:787	arg1	design					750:755	central composite design	732:755	central composite design of response surface methodology for assessing its growth properties in presence of Th	732:841	AM7 was characterized physico-chemically and its culture medium was optimized using central composite design of response surface methodology for assessing its growth properties in presence of Th.
31954311	8	31	theme	EPS-Th	1183:1188	arg1	binding					1190:1196	EPS-Th binding	1183:1196	EPS-Th binding	1183:1196	FTIR spectroscopy revealed the functional groups of EPS involved in EPS-Th binding.
31954311	5	32	theme	composite	740:748	arg1	design					750:755	central composite design	732:755	central composite design of response surface methodology for assessing its growth properties in presence of Th	732:841	AM7 was characterized physico-chemically and its culture medium was optimized using central composite design of response surface methodology for assessing its growth properties in presence of Th.
31954311	10	33	theme	Such	1304:1307	arg1	study					1309:1313	Such study	1304:1313	Such study	1304:1313	Such study will help other researchers to strategize an environment-friendly way of radwaste disposal.
31954311	7	34	theme	EPS	1048:1050	arg1	medium					1052:1057	the EPS medium	1044:1057	the EPS medium	1044:1057	On supplementing the EPS medium with Th, no significant decrease in yield was observed.
31954311	0	35	theme	intermedium	56:66	arg1	Characterization					0:15	Characterization	0:15	Characterization of novel thorium tolerant Ochrobactrum intermedium	0:66	Characterization of novel thorium tolerant Ochrobactrum intermedium AM7 in consort with assessing its EPS-Thorium binding.
31954311	3	36	theme	bacterial	332:340	arg1	flora					342:346	the bacterial flora	328:346	the bacterial flora	328:346	In the present study the bacterial flora, from soil sample collected from an area around atomic power station exposed to radionuclides and heavy metals, was cultivated and assessed for thorium (Th) tolerance.
31954311	7	37	theme	significant	1071:1081	arg1	decrease					1083:1090	no significant decrease	1068:1090	no significant decrease in yield	1068:1099	On supplementing the EPS medium with Th, no significant decrease in yield was observed.
31954311	3	38	theme	present	314:320	arg1	study					322:326	the present study	310:326	the present study	310:326	In the present study the bacterial flora, from soil sample collected from an area around atomic power station exposed to radionuclides and heavy metals, was cultivated and assessed for thorium (Th) tolerance.
31954311	1	39	theme	radioactive	134:144	arg1	waste					146:150	radioactive waste	134:150	radioactive waste	134:150	Currently, radioactive waste is disposed primarily by burial in a deep geological repository.
31954311	6	40	theme	medium	1009:1014	arg1	component					1016:1024	each medium component	1004:1024	each medium component	1004:1024	The strain also showed exceptional exopolysaccharide (EPS) production and its yield was further analyzed using one factor study to investigate the influence of each medium component.
31954311	0	41	dep	novel	20:24	arg1	tolerant					34:41	tolerant	34:41	tolerant	34:41	Characterization of novel thorium tolerant Ochrobactrum intermedium AM7 in consort with assessing its EPS-Thorium binding.
31954311	3	42	theme	thorium	492:498	arg1	tolerance					505:513	thorium (Th) tolerance	492:513	thorium (Th) tolerance	492:513	In the present study the bacterial flora, from soil sample collected from an area around atomic power station exposed to radionuclides and heavy metals, was cultivated and assessed for thorium (Th) tolerance.
31954311	5	43	theme	central	732:738	arg1	design					750:755	central composite design	732:755	central composite design of response surface methodology for assessing its growth properties in presence of Th	732:841	AM7 was characterized physico-chemically and its culture medium was optimized using central composite design of response surface methodology for assessing its growth properties in presence of Th.
31954311	6	44	theme	component	1016:1024	arg1	influence					991:999	the influence	987:999	the influence of each medium component	987:1024	The strain also showed exceptional exopolysaccharide (EPS) production and its yield was further analyzed using one factor study to investigate the influence of each medium component.
31954311	1	45	theme	geological	194:203	arg1	repository					205:214	a deep geological repository	187:214	a deep geological repository	187:214	Currently, radioactive waste is disposed primarily by burial in a deep geological repository.
31954311	3	46	theme	soil	354:357	arg1	sample					359:364	soil sample	354:364	soil sample collected from an area around atomic power station exposed to radionuclides and heavy metals	354:457	In the present study the bacterial flora, from soil sample collected from an area around atomic power station exposed to radionuclides and heavy metals, was cultivated and assessed for thorium (Th) tolerance.
31954311	8	47	theme	EPS	1167:1169	arg1	EPS					1167:1169	EPS	1167:1169	EPS involved in EPS-Th binding	1167:1196	FTIR spectroscopy revealed the functional groups of EPS involved in EPS-Th binding.
31954311	8	47	theme	EPS	1167:1169	arg1	groups					1157:1162	the functional groups	1142:1162	the functional groups of EPS involved in EPS-Th binding	1142:1196	FTIR spectroscopy revealed the functional groups of EPS involved in EPS-Th binding.
33965496	0	0	theme	sensory	87:93	arg1	perception					95:104	sweet sensory perception	81:104	sweet sensory perception	81:104	Starch molecular configuration and starch-sugar homeostasis: Key determinants of sweet sensory perception and starch hydrolysis in pearl millet (Pennisetum glaucum).
33965496	2	1	theme	IGP	490:492	arg1	sweetness					505:513	sweetness	505:513	the key determinants of IGP as well as sweetness in different Pearl millet (PM) genotypes	466:554	The objective of the research was to understand the key determinants of IGP as well as sweetness in different Pearl millet (PM) genotypes.
33965496	2	1	theme	IGP	490:492	arg1	determinants					474:485	the key determinants	466:485	the key determinants of IGP as well as sweetness in different Pearl millet (PM) genotypes	466:554	The objective of the research was to understand the key determinants of IGP as well as sweetness in different Pearl millet (PM) genotypes.
33965496	8	2	theme	starch	1449:1454	arg1	compactness					1434:1444	least molecular compactness	1418:1444	least molecular compactness of starch	1418:1454	Overall our results suggest higher sweet sensory perception of Jafarabadi Bajra which is contributed by the matrix composition with least molecular compactness of starch.
33965496	0	3	theme	sweet	81:85	arg1	perception					95:104	sweet sensory perception	81:104	sweet sensory perception	81:104	Starch molecular configuration and starch-sugar homeostasis: Key determinants of sweet sensory perception and starch hydrolysis in pearl millet (Pennisetum glaucum).
33965496	3	4	theme	total	650:654	arg1	sugars					664:669	total soluble sugars	650:669	total soluble sugars (TSS)	650:675	To understand the intricate balance between starch and sugar, total starch content (TSC) and total soluble sugars (TSS) were evaluated.
33965496	3	4	theme	total	650:654	arg1	TSS					672:674	TSS	672:674	TSS	672:674	To understand the intricate balance between starch and sugar, total starch content (TSC) and total soluble sugars (TSS) were evaluated.
33965496	3	5	theme	total	619:623	arg1	TSC					641:643	TSC	641:643	TSC	641:643	To understand the intricate balance between starch and sugar, total starch content (TSC) and total soluble sugars (TSS) were evaluated.
33965496	3	5	theme	total	619:623	arg1	content					632:638	total starch content	619:638	total starch content (TSC)	619:644	To understand the intricate balance between starch and sugar, total starch content (TSC) and total soluble sugars (TSS) were evaluated.
33965496	7	6	from	starch	1253:1258	arg1	Bajra					1279:1283	Bajra	1279:1283	Bajra	1279:1283	In vitro starch hydrolyzation kinetics carried out to determine IGP, revealed a maximum of 77.05% IGP with minimum 1.42% resistant starch (RS) in Jafarabadi Bajra.
33965496	2	7	theme	different	518:526	arg1	genotypes					546:554	different Pearl millet (PM) genotypes	518:554	different Pearl millet (PM) genotypes	518:554	The objective of the research was to understand the key determinants of IGP as well as sweetness in different Pearl millet (PM) genotypes.
33965496	3	8	theme	soluble	656:662	arg1	sugars					664:669	total soluble sugars	650:669	total soluble sugars (TSS)	650:675	To understand the intricate balance between starch and sugar, total starch content (TSC) and total soluble sugars (TSS) were evaluated.
33965496	3	8	theme	soluble	656:662	arg1	TSS					672:674	TSS	672:674	TSS	672:674	To understand the intricate balance between starch and sugar, total starch content (TSC) and total soluble sugars (TSS) were evaluated.
33965496	5	9	theme	starch	871:876	arg1	configuration					854:866	compact molecular configuration	836:866	compact molecular configuration of starch	836:876	Considering the role of compact molecular configuration of starch towards digestibility, X-ray powder diffraction (XRD) analysis was performed.
33965496	0	10	theme	perception	95:104	arg1	determinants					65:76	Key determinants	61:76	Starch molecular configuration and starch-sugar homeostasis: Key determinants of sweet sensory perception and starch hydrolysis in pearl millet (Pennisetum glaucum).	0:164	Starch molecular configuration and starch-sugar homeostasis: Key determinants of sweet sensory perception and starch hydrolysis in pearl millet (Pennisetum glaucum).
33965496	4	11	theme	sucrose	764:770	arg1	concentrations					700:713	Higher concentrations	693:713	Higher concentrations of TSC (67.8%), TSS (2.75%), glucose (0.78%) and sucrose (1.68%)	693:778	Higher concentrations of TSC (67.8%), TSS (2.75%), glucose (0.78%) and sucrose (1.68%) were found in Jafarabadi Bajra.
33965496	6	12	theme	least	1072:1076	arg1	%					1080:1080	the least CD%	1068:1080	the least CD% (53.53%)	1068:1089	A-type crystallinity with crystallinity degree (CD %) ranged from 53.53-62.63% among different genotypes, where the least CD% (53.53%) was found in Jafarabadi Bajra.
33965496	6	12	theme	least	1072:1076	arg1	%					1088:1088	53.53%	1083:1088	53.53%	1083:1088	A-type crystallinity with crystallinity degree (CD %) ranged from 53.53-62.63% among different genotypes, where the least CD% (53.53%) was found in Jafarabadi Bajra.
33965496	4	13	theme	TSC	718:720	arg1	concentrations					700:713	Higher concentrations	693:713	Higher concentrations of TSC (67.8%), TSS (2.75%), glucose (0.78%) and sucrose (1.68%)	693:778	Higher concentrations of TSC (67.8%), TSS (2.75%), glucose (0.78%) and sucrose (1.68%) were found in Jafarabadi Bajra.
33965496	4	14	located	found	785:789	arg2	concentrations					700:713	Higher concentrations	693:713	Higher concentrations of TSC (67.8%), TSS (2.75%), glucose (0.78%) and sucrose (1.68%)	693:778	Higher concentrations of TSC (67.8%), TSS (2.75%), glucose (0.78%) and sucrose (1.68%) were found in Jafarabadi Bajra.
33965496	4	14	located	found	785:789	arg1	Bajra					805:809	Bajra	805:809	Bajra	805:809	Higher concentrations of TSC (67.8%), TSS (2.75%), glucose (0.78%) and sucrose (1.68%) were found in Jafarabadi Bajra.
33965496	1	15	theme	sweet	289:293	arg1	perception					303:312	sweet sensory perception	289:312	sweet sensory perception	289:312	Starch-sugar homeostasis and starch molecular configuration regulates the dynamics of starch digestibility which result in sweet sensory perception and eliciting glycemic response, which has been measured in vitro as inherent glycemic potential (IGP).
33965496	0	16	theme	hydrolysis	117:126	arg1	determinants					65:76	Key determinants	61:76	Starch molecular configuration and starch-sugar homeostasis: Key determinants of sweet sensory perception and starch hydrolysis in pearl millet (Pennisetum glaucum).	0:164	Starch molecular configuration and starch-sugar homeostasis: Key determinants of sweet sensory perception and starch hydrolysis in pearl millet (Pennisetum glaucum).
33965496	6	17	located	found	1095:1099	arg2	%					1088:1088	53.53%	1083:1088	53.53%	1083:1088	A-type crystallinity with crystallinity degree (CD %) ranged from 53.53-62.63% among different genotypes, where the least CD% (53.53%) was found in Jafarabadi Bajra.
33965496	6	17	located	found	1095:1099	arg1	Bajra					1115:1119	Bajra	1115:1119	Bajra	1115:1119	A-type crystallinity with crystallinity degree (CD %) ranged from 53.53-62.63% among different genotypes, where the least CD% (53.53%) was found in Jafarabadi Bajra.
33965496	6	17	located	found	1095:1099	arg2	%					1080:1080	the least CD%	1068:1080	the least CD% (53.53%)	1068:1089	A-type crystallinity with crystallinity degree (CD %) ranged from 53.53-62.63% among different genotypes, where the least CD% (53.53%) was found in Jafarabadi Bajra.
33965496	7	18	theme	%	1218:1218	arg1	IGP					1220:1222	77.05% IGP	1213:1222	77.05% IGP	1213:1222	In vitro starch hydrolyzation kinetics carried out to determine IGP, revealed a maximum of 77.05% IGP with minimum 1.42% resistant starch (RS) in Jafarabadi Bajra.
33965496	6	19	theme	A-type	956:961	arg1	crystallinity					963:975	A-type crystallinity	956:975	A-type crystallinity with crystallinity degree (CD %)	956:1008	A-type crystallinity with crystallinity degree (CD %) ranged from 53.53-62.63% among different genotypes, where the least CD% (53.53%) was found in Jafarabadi Bajra.
33965496	5	20	theme	molecular	844:852	arg1	configuration					854:866	compact molecular configuration	836:866	compact molecular configuration of starch	836:876	Considering the role of compact molecular configuration of starch towards digestibility, X-ray powder diffraction (XRD) analysis was performed.
33965496	1	21	theme	Starch-sugar	166:177	arg1	homeostasis					179:189	Starch-sugar homeostasis	166:189	Starch-sugar homeostasis	166:189	Starch-sugar homeostasis and starch molecular configuration regulates the dynamics of starch digestibility which result in sweet sensory perception and eliciting glycemic response, which has been measured in vitro as inherent glycemic potential (IGP).
33965496	0	22	theme	starch	110:115	arg1	hydrolysis					117:126	starch hydrolysis	110:126	starch hydrolysis	110:126	Starch molecular configuration and starch-sugar homeostasis: Key determinants of sweet sensory perception and starch hydrolysis in pearl millet (Pennisetum glaucum).
33965496	1	23	theme	inherent	383:390	arg1	IGP					412:414	IGP	412:414	IGP	412:414	Starch-sugar homeostasis and starch molecular configuration regulates the dynamics of starch digestibility which result in sweet sensory perception and eliciting glycemic response, which has been measured in vitro as inherent glycemic potential (IGP).
33965496	1	23	theme	inherent	383:390	arg1	response					337:344	glycemic response	328:344	glycemic response	328:344	Starch-sugar homeostasis and starch molecular configuration regulates the dynamics of starch digestibility which result in sweet sensory perception and eliciting glycemic response, which has been measured in vitro as inherent glycemic potential (IGP).
33965496	1	23	theme	inherent	383:390	arg1	potential					401:409	inherent glycemic potential	383:409	inherent glycemic potential (IGP)	383:415	Starch-sugar homeostasis and starch molecular configuration regulates the dynamics of starch digestibility which result in sweet sensory perception and eliciting glycemic response, which has been measured in vitro as inherent glycemic potential (IGP).
33965496	4	24	theme	glucose	744:750	arg1	concentrations					700:713	Higher concentrations	693:713	Higher concentrations of TSC (67.8%), TSS (2.75%), glucose (0.78%) and sucrose (1.68%)	693:778	Higher concentrations of TSC (67.8%), TSS (2.75%), glucose (0.78%) and sucrose (1.68%) were found in Jafarabadi Bajra.
33965496	5	25	theme	configuration	854:866	arg1	role					828:831	the role	824:831	the role of compact molecular configuration of starch towards digestibility	824:898	Considering the role of compact molecular configuration of starch towards digestibility, X-ray powder diffraction (XRD) analysis was performed.
33965496	7	26	theme	minimum	1229:1235	arg1	RS					1261:1262	RS	1261:1262	RS	1261:1262	In vitro starch hydrolyzation kinetics carried out to determine IGP, revealed a maximum of 77.05% IGP with minimum 1.42% resistant starch (RS) in Jafarabadi Bajra.
33965496	7	26	theme	minimum	1229:1235	arg1	starch					1253:1258	minimum 1.42% resistant starch	1229:1258	minimum 1.42% resistant starch (RS) in Jafarabadi Bajra	1229:1283	In vitro starch hydrolyzation kinetics carried out to determine IGP, revealed a maximum of 77.05% IGP with minimum 1.42% resistant starch (RS) in Jafarabadi Bajra.
33965496	0	27	theme	molecular	7:15	arg1	configuration					17:29	Starch molecular configuration	0:29	Starch molecular configuration	0:29	Starch molecular configuration and starch-sugar homeostasis: Key determinants of sweet sensory perception and starch hydrolysis in pearl millet (Pennisetum glaucum).
33965496	1	28	theme	glycemic	392:399	arg1	IGP					412:414	IGP	412:414	IGP	412:414	Starch-sugar homeostasis and starch molecular configuration regulates the dynamics of starch digestibility which result in sweet sensory perception and eliciting glycemic response, which has been measured in vitro as inherent glycemic potential (IGP).
33965496	1	28	theme	glycemic	392:399	arg1	response					337:344	glycemic response	328:344	glycemic response	328:344	Starch-sugar homeostasis and starch molecular configuration regulates the dynamics of starch digestibility which result in sweet sensory perception and eliciting glycemic response, which has been measured in vitro as inherent glycemic potential (IGP).
33965496	1	28	theme	glycemic	392:399	arg1	potential					401:409	inherent glycemic potential	383:409	inherent glycemic potential (IGP)	383:415	Starch-sugar homeostasis and starch molecular configuration regulates the dynamics of starch digestibility which result in sweet sensory perception and eliciting glycemic response, which has been measured in vitro as inherent glycemic potential (IGP).
33965496	7	29	dep	In	1122:1123	arg1	vitro					1125:1129	vitro	1125:1129	vitro	1125:1129	In vitro starch hydrolyzation kinetics carried out to determine IGP, revealed a maximum of 77.05% IGP with minimum 1.42% resistant starch (RS) in Jafarabadi Bajra.
33965496	4	30	theme	Higher	693:698	arg1	concentrations					700:713	Higher concentrations	693:713	Higher concentrations of TSC (67.8%), TSS (2.75%), glucose (0.78%) and sucrose (1.68%)	693:778	Higher concentrations of TSC (67.8%), TSS (2.75%), glucose (0.78%) and sucrose (1.68%) were found in Jafarabadi Bajra.
33965496	6	31	with	crystallinity	963:975	arg1	degree					996:1001	crystallinity degree	982:1001	crystallinity degree (CD %)	982:1008	A-type crystallinity with crystallinity degree (CD %) ranged from 53.53-62.63% among different genotypes, where the least CD% (53.53%) was found in Jafarabadi Bajra.
33965496	6	31	with	crystallinity	963:975	arg1	%					1007:1007	CD %	1004:1007	CD %	1004:1007	A-type crystallinity with crystallinity degree (CD %) ranged from 53.53-62.63% among different genotypes, where the least CD% (53.53%) was found in Jafarabadi Bajra.
33965496	0	32	theme	Starch	0:5	arg1	configuration					17:29	Starch molecular configuration	0:29	Starch molecular configuration	0:29	Starch molecular configuration and starch-sugar homeostasis: Key determinants of sweet sensory perception and starch hydrolysis in pearl millet (Pennisetum glaucum).
33965496	1	33	theme	sensory	295:301	arg1	perception					303:312	sweet sensory perception	289:312	sweet sensory perception	289:312	Starch-sugar homeostasis and starch molecular configuration regulates the dynamics of starch digestibility which result in sweet sensory perception and eliciting glycemic response, which has been measured in vitro as inherent glycemic potential (IGP).
33965496	7	34	theme	1.42	1237:1240	arg1	%					1241:1241	%	1241:1241	%	1241:1241	In vitro starch hydrolyzation kinetics carried out to determine IGP, revealed a maximum of 77.05% IGP with minimum 1.42% resistant starch (RS) in Jafarabadi Bajra.
33965496	1	35	theme	starch	195:200	arg1	configuration					212:224	starch molecular configuration	195:224	starch molecular configuration	195:224	Starch-sugar homeostasis and starch molecular configuration regulates the dynamics of starch digestibility which result in sweet sensory perception and eliciting glycemic response, which has been measured in vitro as inherent glycemic potential (IGP).
33965496	2	36	theme	research	439:446	arg1	objective					422:430	The objective	418:430	The objective of the research	418:446	The objective of the research was to understand the key determinants of IGP as well as sweetness in different Pearl millet (PM) genotypes.
33965496	8	37	theme	Bajra	1360:1364	arg1	perception					1335:1344	higher sweet sensory perception	1314:1344	higher sweet sensory perception of Jafarabadi Bajra which is contributed by the matrix composition with least molecular compactness of starch	1314:1454	Overall our results suggest higher sweet sensory perception of Jafarabadi Bajra which is contributed by the matrix composition with least molecular compactness of starch.
33965496	0	38	theme	pearl	131:135	arg1	millet					137:142	pearl millet	131:142	pearl millet (Pennisetum glaucum)	131:163	Starch molecular configuration and starch-sugar homeostasis: Key determinants of sweet sensory perception and starch hydrolysis in pearl millet (Pennisetum glaucum).
33965496	7	39	theme	IGP	1220:1222	arg1	maximum					1202:1208	a maximum	1200:1208	a maximum of 77.05% IGP with minimum 1.42% resistant starch (RS) in Jafarabadi Bajra	1200:1283	In vitro starch hydrolyzation kinetics carried out to determine IGP, revealed a maximum of 77.05% IGP with minimum 1.42% resistant starch (RS) in Jafarabadi Bajra.
33965496	7	40	theme	77.05	1213:1217	arg1	%					1218:1218	%	1218:1218	%	1218:1218	In vitro starch hydrolyzation kinetics carried out to determine IGP, revealed a maximum of 77.05% IGP with minimum 1.42% resistant starch (RS) in Jafarabadi Bajra.
33965496	1	41	theme	molecular	202:210	arg1	configuration					212:224	starch molecular configuration	195:224	starch molecular configuration	195:224	Starch-sugar homeostasis and starch molecular configuration regulates the dynamics of starch digestibility which result in sweet sensory perception and eliciting glycemic response, which has been measured in vitro as inherent glycemic potential (IGP).
33965496	8	42	theme	sensory	1327:1333	arg1	perception					1335:1344	higher sweet sensory perception	1314:1344	higher sweet sensory perception of Jafarabadi Bajra which is contributed by the matrix composition with least molecular compactness of starch	1314:1454	Overall our results suggest higher sweet sensory perception of Jafarabadi Bajra which is contributed by the matrix composition with least molecular compactness of starch.
33965496	6	43	theme	different	1041:1049	arg1	genotypes					1051:1059	different genotypes	1041:1059	different genotypes	1041:1059	A-type crystallinity with crystallinity degree (CD %) ranged from 53.53-62.63% among different genotypes, where the least CD% (53.53%) was found in Jafarabadi Bajra.
33965496	3	44	theme	starch	625:630	arg1	TSC					641:643	TSC	641:643	TSC	641:643	To understand the intricate balance between starch and sugar, total starch content (TSC) and total soluble sugars (TSS) were evaluated.
33965496	3	44	theme	starch	625:630	arg1	content					632:638	total starch content	619:638	total starch content (TSC)	619:644	To understand the intricate balance between starch and sugar, total starch content (TSC) and total soluble sugars (TSS) were evaluated.
33965496	2	45	theme	key	470:472	arg1	determinants					474:485	the key determinants	466:485	the key determinants of IGP as well as sweetness in different Pearl millet (PM) genotypes	466:554	The objective of the research was to understand the key determinants of IGP as well as sweetness in different Pearl millet (PM) genotypes.
33965496	5	46	theme	compact	836:842	arg1	configuration					854:866	compact molecular configuration	836:866	compact molecular configuration of starch	836:876	Considering the role of compact molecular configuration of starch towards digestibility, X-ray powder diffraction (XRD) analysis was performed.
33965496	9	47	dep	parameters	1504:1513	arg1	RS					1526:1527	RS	1526:1527	RS	1526:1527	Also, the interdependence among starch quality parameters; CD%, IGP, RS and amylose has also been discussed.
33965496	9	47	dep	parameters	1504:1513	arg1	IGP					1521:1523	IGP	1521:1523	IGP	1521:1523	Also, the interdependence among starch quality parameters; CD%, IGP, RS and amylose has also been discussed.
33965496	9	47	dep	parameters	1504:1513	arg1	%					1518:1518	CD%	1516:1518	CD%	1516:1518	Also, the interdependence among starch quality parameters; CD%, IGP, RS and amylose has also been discussed.
33965496	9	47	dep	parameters	1504:1513	arg1	amylose					1533:1539	amylose	1533:1539	amylose	1533:1539	Also, the interdependence among starch quality parameters; CD%, IGP, RS and amylose has also been discussed.
33965496	2	48	theme	millet	534:539	arg1	genotypes					546:554	different Pearl millet (PM) genotypes	518:554	different Pearl millet (PM) genotypes	518:554	The objective of the research was to understand the key determinants of IGP as well as sweetness in different Pearl millet (PM) genotypes.
33965496	3	49	theme	intricate	575:583	arg1	balance					585:591	the intricate balance	571:591	the intricate balance between starch and sugar	571:616	To understand the intricate balance between starch and sugar, total starch content (TSC) and total soluble sugars (TSS) were evaluated.
33965496	0	50	theme	starch-sugar	35:46	arg1	homeostasis					48:58	starch-sugar homeostasis	35:58	starch-sugar homeostasis	35:58	Starch molecular configuration and starch-sugar homeostasis: Key determinants of sweet sensory perception and starch hydrolysis in pearl millet (Pennisetum glaucum).
33965496	1	51	theme	glycemic	328:335	arg1	response					337:344	glycemic response	328:344	glycemic response	328:344	Starch-sugar homeostasis and starch molecular configuration regulates the dynamics of starch digestibility which result in sweet sensory perception and eliciting glycemic response, which has been measured in vitro as inherent glycemic potential (IGP).
33965496	1	51	theme	glycemic	328:335	arg1	potential					401:409	inherent glycemic potential	383:409	inherent glycemic potential (IGP)	383:415	Starch-sugar homeostasis and starch molecular configuration regulates the dynamics of starch digestibility which result in sweet sensory perception and eliciting glycemic response, which has been measured in vitro as inherent glycemic potential (IGP).
33965496	2	52	theme	Pearl	528:532	arg1	genotypes					546:554	different Pearl millet (PM) genotypes	518:554	different Pearl millet (PM) genotypes	518:554	The objective of the research was to understand the key determinants of IGP as well as sweetness in different Pearl millet (PM) genotypes.
33965496	5	53	theme	X-ray	901:905	arg1	XRD					927:929	XRD	927:929	XRD	927:929	Considering the role of compact molecular configuration of starch towards digestibility, X-ray powder diffraction (XRD) analysis was performed.
33965496	5	53	theme	X-ray	901:905	arg1	diffraction					914:924	X-ray powder diffraction	901:924	X-ray powder diffraction (XRD) analysis	901:939	Considering the role of compact molecular configuration of starch towards digestibility, X-ray powder diffraction (XRD) analysis was performed.
33965496	7	54	theme	starch	1131:1136	arg1	kinetics					1152:1159	In vitro starch hydrolyzation kinetics	1122:1159	In vitro starch hydrolyzation kinetics	1122:1159	In vitro starch hydrolyzation kinetics carried out to determine IGP, revealed a maximum of 77.05% IGP with minimum 1.42% resistant starch (RS) in Jafarabadi Bajra.
33965496	0	55	theme	Key	61:63	arg1	determinants					65:76	Key determinants	61:76	Starch molecular configuration and starch-sugar homeostasis: Key determinants of sweet sensory perception and starch hydrolysis in pearl millet (Pennisetum glaucum).	0:164	Starch molecular configuration and starch-sugar homeostasis: Key determinants of sweet sensory perception and starch hydrolysis in pearl millet (Pennisetum glaucum).
33965496	7	56	with	maximum	1202:1208	arg1	RS					1261:1262	RS	1261:1262	RS	1261:1262	In vitro starch hydrolyzation kinetics carried out to determine IGP, revealed a maximum of 77.05% IGP with minimum 1.42% resistant starch (RS) in Jafarabadi Bajra.
33965496	7	56	with	maximum	1202:1208	arg1	starch					1253:1258	minimum 1.42% resistant starch	1229:1258	minimum 1.42% resistant starch (RS) in Jafarabadi Bajra	1229:1283	In vitro starch hydrolyzation kinetics carried out to determine IGP, revealed a maximum of 77.05% IGP with minimum 1.42% resistant starch (RS) in Jafarabadi Bajra.
33965496	0	57	from	determinants	65:76	arg1	millet					137:142	pearl millet	131:142	pearl millet (Pennisetum glaucum)	131:163	Starch molecular configuration and starch-sugar homeostasis: Key determinants of sweet sensory perception and starch hydrolysis in pearl millet (Pennisetum glaucum).
33965496	5	58	theme	powder	907:912	arg1	XRD					927:929	XRD	927:929	XRD	927:929	Considering the role of compact molecular configuration of starch towards digestibility, X-ray powder diffraction (XRD) analysis was performed.
33965496	5	58	theme	powder	907:912	arg1	diffraction					914:924	X-ray powder diffraction	901:924	X-ray powder diffraction (XRD) analysis	901:939	Considering the role of compact molecular configuration of starch towards digestibility, X-ray powder diffraction (XRD) analysis was performed.
33965496	2	59	from	determinants	474:485	arg1	genotypes					546:554	different Pearl millet (PM) genotypes	518:554	different Pearl millet (PM) genotypes	518:554	The objective of the research was to understand the key determinants of IGP as well as sweetness in different Pearl millet (PM) genotypes.
33965496	8	60	theme	higher	1314:1319	arg1	perception					1335:1344	higher sweet sensory perception	1314:1344	higher sweet sensory perception of Jafarabadi Bajra which is contributed by the matrix composition with least molecular compactness of starch	1314:1454	Overall our results suggest higher sweet sensory perception of Jafarabadi Bajra which is contributed by the matrix composition with least molecular compactness of starch.
33965496	2	61	theme	PM	542:543	arg1	genotypes					546:554	different Pearl millet (PM) genotypes	518:554	different Pearl millet (PM) genotypes	518:554	The objective of the research was to understand the key determinants of IGP as well as sweetness in different Pearl millet (PM) genotypes.
33965496	2	62	from	sweetness	505:513	arg1	genotypes					546:554	different Pearl millet (PM) genotypes	518:554	different Pearl millet (PM) genotypes	518:554	The objective of the research was to understand the key determinants of IGP as well as sweetness in different Pearl millet (PM) genotypes.
33965496	5	63	theme	diffraction	914:924	arg1	analysis					932:939	X-ray powder diffraction (XRD) analysis	901:939	X-ray powder diffraction (XRD) analysis	901:939	Considering the role of compact molecular configuration of starch towards digestibility, X-ray powder diffraction (XRD) analysis was performed.
33965496	7	64	theme	In	1122:1123	arg1	kinetics					1152:1159	In vitro starch hydrolyzation kinetics	1122:1159	In vitro starch hydrolyzation kinetics	1122:1159	In vitro starch hydrolyzation kinetics carried out to determine IGP, revealed a maximum of 77.05% IGP with minimum 1.42% resistant starch (RS) in Jafarabadi Bajra.
33965496	8	65	theme	matrix	1394:1399	arg1	composition					1401:1411	the matrix composition	1390:1411	the matrix composition with least molecular compactness of starch	1390:1454	Overall our results suggest higher sweet sensory perception of Jafarabadi Bajra which is contributed by the matrix composition with least molecular compactness of starch.
33965496	8	66	with	composition	1401:1411	arg1	compactness					1434:1444	least molecular compactness	1418:1444	least molecular compactness of starch	1418:1454	Overall our results suggest higher sweet sensory perception of Jafarabadi Bajra which is contributed by the matrix composition with least molecular compactness of starch.
33965496	6	67	theme	CD	1078:1079	arg1	%					1080:1080	the least CD%	1068:1080	the least CD% (53.53%)	1068:1089	A-type crystallinity with crystallinity degree (CD %) ranged from 53.53-62.63% among different genotypes, where the least CD% (53.53%) was found in Jafarabadi Bajra.
33965496	6	67	theme	CD	1078:1079	arg1	%					1088:1088	53.53%	1083:1088	53.53%	1083:1088	A-type crystallinity with crystallinity degree (CD %) ranged from 53.53-62.63% among different genotypes, where the least CD% (53.53%) was found in Jafarabadi Bajra.
33965496	7	68	theme	%	1241:1241	arg1	RS					1261:1262	RS	1261:1262	RS	1261:1262	In vitro starch hydrolyzation kinetics carried out to determine IGP, revealed a maximum of 77.05% IGP with minimum 1.42% resistant starch (RS) in Jafarabadi Bajra.
33965496	7	68	theme	%	1241:1241	arg1	starch					1253:1258	minimum 1.42% resistant starch	1229:1258	minimum 1.42% resistant starch (RS) in Jafarabadi Bajra	1229:1283	In vitro starch hydrolyzation kinetics carried out to determine IGP, revealed a maximum of 77.05% IGP with minimum 1.42% resistant starch (RS) in Jafarabadi Bajra.
33965496	6	69	theme	CD	1004:1005	arg1	degree					996:1001	crystallinity degree	982:1001	crystallinity degree (CD %)	982:1008	A-type crystallinity with crystallinity degree (CD %) ranged from 53.53-62.63% among different genotypes, where the least CD% (53.53%) was found in Jafarabadi Bajra.
33965496	6	69	theme	CD	1004:1005	arg1	%					1007:1007	CD %	1004:1007	CD %	1004:1007	A-type crystallinity with crystallinity degree (CD %) ranged from 53.53-62.63% among different genotypes, where the least CD% (53.53%) was found in Jafarabadi Bajra.
33965496	8	70	theme	least	1418:1422	arg1	compactness					1434:1444	least molecular compactness	1418:1444	least molecular compactness of starch	1418:1454	Overall our results suggest higher sweet sensory perception of Jafarabadi Bajra which is contributed by the matrix composition with least molecular compactness of starch.
33965496	7	71	theme	resistant	1243:1251	arg1	RS					1261:1262	RS	1261:1262	RS	1261:1262	In vitro starch hydrolyzation kinetics carried out to determine IGP, revealed a maximum of 77.05% IGP with minimum 1.42% resistant starch (RS) in Jafarabadi Bajra.
33965496	7	71	theme	resistant	1243:1251	arg1	starch					1253:1258	minimum 1.42% resistant starch	1229:1258	minimum 1.42% resistant starch (RS) in Jafarabadi Bajra	1229:1283	In vitro starch hydrolyzation kinetics carried out to determine IGP, revealed a maximum of 77.05% IGP with minimum 1.42% resistant starch (RS) in Jafarabadi Bajra.
33965496	0	72	dep	configuration	17:29	arg1	determinants					65:76	Key determinants	61:76	Starch molecular configuration and starch-sugar homeostasis: Key determinants of sweet sensory perception and starch hydrolysis in pearl millet (Pennisetum glaucum).	0:164	Starch molecular configuration and starch-sugar homeostasis: Key determinants of sweet sensory perception and starch hydrolysis in pearl millet (Pennisetum glaucum).
33965496	6	73	theme	crystallinity	982:994	arg1	degree					996:1001	crystallinity degree	982:1001	crystallinity degree (CD %)	982:1008	A-type crystallinity with crystallinity degree (CD %) ranged from 53.53-62.63% among different genotypes, where the least CD% (53.53%) was found in Jafarabadi Bajra.
33965496	6	73	theme	crystallinity	982:994	arg1	%					1007:1007	CD %	1004:1007	CD %	1004:1007	A-type crystallinity with crystallinity degree (CD %) ranged from 53.53-62.63% among different genotypes, where the least CD% (53.53%) was found in Jafarabadi Bajra.
33965496	8	74	theme	sweet	1321:1325	arg1	perception					1335:1344	higher sweet sensory perception	1314:1344	higher sweet sensory perception of Jafarabadi Bajra which is contributed by the matrix composition with least molecular compactness of starch	1314:1454	Overall our results suggest higher sweet sensory perception of Jafarabadi Bajra which is contributed by the matrix composition with least molecular compactness of starch.
33965496	7	75	theme	hydrolyzation	1138:1150	arg1	kinetics					1152:1159	In vitro starch hydrolyzation kinetics	1122:1159	In vitro starch hydrolyzation kinetics	1122:1159	In vitro starch hydrolyzation kinetics carried out to determine IGP, revealed a maximum of 77.05% IGP with minimum 1.42% resistant starch (RS) in Jafarabadi Bajra.
33965496	9	76	theme	CD	1516:1517	arg1	%					1518:1518	CD%	1516:1518	CD%	1516:1518	Also, the interdependence among starch quality parameters; CD%, IGP, RS and amylose has also been discussed.
33965496	9	77	theme	quality	1496:1502	arg1	parameters					1504:1513	starch quality parameters	1489:1513	starch quality parameters; CD%, IGP, RS and amylose	1489:1539	Also, the interdependence among starch quality parameters; CD%, IGP, RS and amylose has also been discussed.
33965496	1	78	theme	starch	252:257	arg1	digestibility					259:271	starch digestibility	252:271	starch digestibility which result in sweet sensory perception and eliciting glycemic response, which has been measured in vitro as inherent glycemic potential (IGP)	252:415	Starch-sugar homeostasis and starch molecular configuration regulates the dynamics of starch digestibility which result in sweet sensory perception and eliciting glycemic response, which has been measured in vitro as inherent glycemic potential (IGP).
33965496	9	79	theme	starch	1489:1494	arg1	parameters					1504:1513	starch quality parameters	1489:1513	starch quality parameters; CD%, IGP, RS and amylose	1489:1539	Also, the interdependence among starch quality parameters; CD%, IGP, RS and amylose has also been discussed.
33965496	4	80	theme	TSS	731:733	arg1	concentrations					700:713	Higher concentrations	693:713	Higher concentrations of TSC (67.8%), TSS (2.75%), glucose (0.78%) and sucrose (1.68%)	693:778	Higher concentrations of TSC (67.8%), TSS (2.75%), glucose (0.78%) and sucrose (1.68%) were found in Jafarabadi Bajra.
33965496	0	81	dep	millet	137:142	arg1	glaucum					156:162	Pennisetum glaucum	145:162	Pennisetum glaucum	145:162	Starch molecular configuration and starch-sugar homeostasis: Key determinants of sweet sensory perception and starch hydrolysis in pearl millet (Pennisetum glaucum).
33965496	8	82	theme	molecular	1424:1432	arg1	compactness					1434:1444	least molecular compactness	1418:1444	least molecular compactness of starch	1418:1454	Overall our results suggest higher sweet sensory perception of Jafarabadi Bajra which is contributed by the matrix composition with least molecular compactness of starch.
33965496	1	83	theme	digestibility	259:271	arg1	dynamics					240:247	the dynamics	236:247	the dynamics of starch digestibility which result in sweet sensory perception and eliciting glycemic response, which has been measured in vitro as inherent glycemic potential (IGP)	236:415	Starch-sugar homeostasis and starch molecular configuration regulates the dynamics of starch digestibility which result in sweet sensory perception and eliciting glycemic response, which has been measured in vitro as inherent glycemic potential (IGP).
33593130	0	0	theme	bredigite/titanium	95:112	arg1	scaffolds					132:140	bredigite/titanium dioxide composite scaffolds	95:140	bredigite/titanium dioxide composite scaffolds	95:140	Investigation of applying chitosan coating on antibacterial and biocompatibility properties of bredigite/titanium dioxide composite scaffolds.
33593130	2	1	theme	porosity	593:600	arg1	porosity					593:600	porosity	593:600	porosity	593:600	The phase structure, fundamental groups, chemical composition, and elemental distribution analysis, morphology and the form of porosity were respectively characterized by XRD, FTIR, EDS, and SEM.
33593130	2	1	theme	porosity	593:600	arg1	analysis					556:563	elemental distribution analysis	533:563	elemental distribution analysis	533:563	The phase structure, fundamental groups, chemical composition, and elemental distribution analysis, morphology and the form of porosity were respectively characterized by XRD, FTIR, EDS, and SEM.
33593130	2	1	theme	porosity	593:600	arg1	composition					516:526	chemical composition	507:526	chemical composition	507:526	The phase structure, fundamental groups, chemical composition, and elemental distribution analysis, morphology and the form of porosity were respectively characterized by XRD, FTIR, EDS, and SEM.
33593130	2	1	theme	porosity	593:600	arg1	morphology					566:575	morphology	566:575	morphology	566:575	The phase structure, fundamental groups, chemical composition, and elemental distribution analysis, morphology and the form of porosity were respectively characterized by XRD, FTIR, EDS, and SEM.
33593130	2	1	theme	porosity	593:600	arg1	structure					476:484	The phase structure	466:484	The phase structure	466:484	The phase structure, fundamental groups, chemical composition, and elemental distribution analysis, morphology and the form of porosity were respectively characterized by XRD, FTIR, EDS, and SEM.
33593130	2	1	theme	porosity	593:600	arg1	form					585:588	the form	581:588	the form of porosity	581:600	The phase structure, fundamental groups, chemical composition, and elemental distribution analysis, morphology and the form of porosity were respectively characterized by XRD, FTIR, EDS, and SEM.
33593130	2	1	theme	porosity	593:600	arg1	groups					499:504	fundamental groups	487:504	fundamental groups	487:504	The phase structure, fundamental groups, chemical composition, and elemental distribution analysis, morphology and the form of porosity were respectively characterized by XRD, FTIR, EDS, and SEM.
33593130	8	2	contain	had	1634:1636	arg1	scaffolds					1624:1632	the scaffolds	1620:1632	the scaffolds	1620:1632	On the other hand, the results of cell compatibility and cell adhesion tests showed that the scaffolds had no toxicity and the growth, proliferation, and adhesion of MG63 bone cells adjacent to the scaffolds was desirable.
33593130	8	2	contain	had	1634:1636	arg2	growth					1658:1663	growth	1658:1663	growth	1658:1663	On the other hand, the results of cell compatibility and cell adhesion tests showed that the scaffolds had no toxicity and the growth, proliferation, and adhesion of MG63 bone cells adjacent to the scaffolds was desirable.
33593130	8	2	contain	had	1634:1636	arg2	toxicity					1641:1648	toxicity	1641:1648	toxicity	1641:1648	On the other hand, the results of cell compatibility and cell adhesion tests showed that the scaffolds had no toxicity and the growth, proliferation, and adhesion of MG63 bone cells adjacent to the scaffolds was desirable.
33593130	6	3	dep	63	1201:1202	arg1	to					1198:1199	to	1198:1199	to	1198:1199	Furthermore, the coated scaffold with chitosan polymer reduced porosity from 83 to 63 percent and a remarkable improvement in compressive strength from 0.585 to 2.339 MPa.
33593130	8	4	theme	tests	1602:1606	arg1	results					1554:1560	the results	1550:1560	the results of cell compatibility and cell adhesion tests	1550:1606	On the other hand, the results of cell compatibility and cell adhesion tests showed that the scaffolds had no toxicity and the growth, proliferation, and adhesion of MG63 bone cells adjacent to the scaffolds was desirable.
33593130	1	5	theme	composite	246:254	arg1	scaffolds					256:264	composite scaffolds	246:264	composite scaffolds of bredigite/titanium dioxide	246:294	In this study by considering the advantages of bredigite (Br) and titanium dioxide (TiO2) bioceramics, composite scaffolds of bredigite/titanium dioxide were prepared by the gelcasting method, then, to improve the mechanical, biological and antibacterial properties, scaffolds were coated with chitosan (Ch) polymer phase.
33593130	2	6	theme	phase	470:474	arg1	structure					476:484	The phase structure	466:484	The phase structure	466:484	The phase structure, fundamental groups, chemical composition, and elemental distribution analysis, morphology and the form of porosity were respectively characterized by XRD, FTIR, EDS, and SEM.
33593130	6	7	theme	compressive	1244:1254	arg1	strength					1256:1263	compressive strength	1244:1263	compressive strength from 0.585	1244:1274	Furthermore, the coated scaffold with chitosan polymer reduced porosity from 83 to 63 percent and a remarkable improvement in compressive strength from 0.585 to 2.339 MPa.
33593130	1	8	theme	chitosan	437:444	arg1	phase					459:463	chitosan (Ch) polymer phase	437:463	chitosan (Ch) polymer phase	437:463	In this study by considering the advantages of bredigite (Br) and titanium dioxide (TiO2) bioceramics, composite scaffolds of bredigite/titanium dioxide were prepared by the gelcasting method, then, to improve the mechanical, biological and antibacterial properties, scaffolds were coated with chitosan (Ch) polymer phase.
33593130	6	9	from	porosity	1181:1188	arg1	0.585					1270:1274	0.585	1270:1274	0.585	1270:1274	Furthermore, the coated scaffold with chitosan polymer reduced porosity from 83 to 63 percent and a remarkable improvement in compressive strength from 0.585 to 2.339 MPa.
33593130	6	9	from	porosity	1181:1188	arg1	strength					1256:1263	compressive strength	1244:1263	compressive strength from 0.585	1244:1274	Furthermore, the coated scaffold with chitosan polymer reduced porosity from 83 to 63 percent and a remarkable improvement in compressive strength from 0.585 to 2.339 MPa.
33593130	0	10	theme	composite	122:130	arg1	scaffolds					132:140	bredigite/titanium dioxide composite scaffolds	95:140	bredigite/titanium dioxide composite scaffolds	95:140	Investigation of applying chitosan coating on antibacterial and biocompatibility properties of bredigite/titanium dioxide composite scaffolds.
33593130	3	11	theme	Mechanical	662:671	arg1	properties					673:682	Mechanical properties	662:682	Mechanical properties	662:682	Mechanical properties and porosity percentage of scaffolds were also measured by the compressive strength test and Archimedean method, respectively.
33593130	4	12	theme	bone	859:862	arg1	cells					871:875	MG63 bone marrow cells	854:875	MG63 bone marrow cells	854:875	In order to verify the cell compatibility, MG63 bone marrow cells were cultured on the surface of the specimens.
33593130	8	13	theme	adjacent	1713:1720	arg1	toxicity					1641:1648	toxicity	1641:1648	toxicity	1641:1648	On the other hand, the results of cell compatibility and cell adhesion tests showed that the scaffolds had no toxicity and the growth, proliferation, and adhesion of MG63 bone cells adjacent to the scaffolds was desirable.
33593130	1	14	dep	study	151:155	arg1	prepared					301:308	prepared	301:308	prepared by the gelcasting method	301:333	In this study by considering the advantages of bredigite (Br) and titanium dioxide (TiO2) bioceramics, composite scaffolds of bredigite/titanium dioxide were prepared by the gelcasting method, then, to improve the mechanical, biological and antibacterial properties, scaffolds were coated with chitosan (Ch) polymer phase.
33593130	8	15	theme	bone	1702:1705	arg1	cells					1707:1711	MG63 bone cells	1697:1711	MG63 bone cells	1697:1711	On the other hand, the results of cell compatibility and cell adhesion tests showed that the scaffolds had no toxicity and the growth, proliferation, and adhesion of MG63 bone cells adjacent to the scaffolds was desirable.
33593130	7	16	theme	aureus	1493:1498	arg1	media					1432:1436	the culture media	1420:1436	the culture media of Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive), respectively	1420:1528	The results of the antibacterial test showed that in composite scaffolds, The diameter of the inhibition zone is 22 and 29 mm, in the culture media of Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive), respectively.
33593130	0	17	theme	dioxide	114:120	arg1	scaffolds					132:140	bredigite/titanium dioxide composite scaffolds	95:140	bredigite/titanium dioxide composite scaffolds	95:140	Investigation of applying chitosan coating on antibacterial and biocompatibility properties of bredigite/titanium dioxide composite scaffolds.
33593130	9	18	theme	tissue	1841:1846	arg1	engineering					1848:1858	bone tissue engineering	1836:1858	bone tissue engineering	1836:1858	Therefore, the scaffold in this study can be used as an ideal scaffold for use in bone tissue engineering.
33593130	4	19	theme	MG63	854:857	arg1	cells					871:875	MG63 bone marrow cells	854:875	MG63 bone marrow cells	854:875	In order to verify the cell compatibility, MG63 bone marrow cells were cultured on the surface of the specimens.
33593130	1	20	theme	Ch	447:448	arg1	phase					459:463	chitosan (Ch) polymer phase	437:463	chitosan (Ch) polymer phase	437:463	In this study by considering the advantages of bredigite (Br) and titanium dioxide (TiO2) bioceramics, composite scaffolds of bredigite/titanium dioxide were prepared by the gelcasting method, then, to improve the mechanical, biological and antibacterial properties, scaffolds were coated with chitosan (Ch) polymer phase.
33593130	7	21	theme	culture	1424:1430	arg1	media					1432:1436	the culture media	1420:1436	the culture media of Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive), respectively	1420:1528	The results of the antibacterial test showed that in composite scaffolds, The diameter of the inhibition zone is 22 and 29 mm, in the culture media of Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive), respectively.
33593130	1	22	theme	bredigite/titanium	269:286	arg1	dioxide					288:294	bredigite/titanium dioxide	269:294	bredigite/titanium dioxide	269:294	In this study by considering the advantages of bredigite (Br) and titanium dioxide (TiO2) bioceramics, composite scaffolds of bredigite/titanium dioxide were prepared by the gelcasting method, then, to improve the mechanical, biological and antibacterial properties, scaffolds were coated with chitosan (Ch) polymer phase.
33593130	1	23	theme	mechanical	357:366	arg1	properties					398:407	the mechanical, biological and antibacterial properties	353:407	the mechanical, biological and antibacterial properties	353:407	In this study by considering the advantages of bredigite (Br) and titanium dioxide (TiO2) bioceramics, composite scaffolds of bredigite/titanium dioxide were prepared by the gelcasting method, then, to improve the mechanical, biological and antibacterial properties, scaffolds were coated with chitosan (Ch) polymer phase.
33593130	6	24	theme	remarkable	1218:1227	arg1	improvement					1229:1239	a remarkable improvement	1216:1239	a remarkable improvement in compressive strength from 0.585 to 2.339 MPa	1216:1287	Furthermore, the coated scaffold with chitosan polymer reduced porosity from 83 to 63 percent and a remarkable improvement in compressive strength from 0.585 to 2.339 MPa.
33593130	5	25	dep	0.687 MPa	1107:1115	arg1	to					1104:1105	to	1104:1105	to	1104:1105	The results showed that the addition of titanium dioxide to the scaffold of bredigite resulted in decrease of porosity and increase of compressive strength of scaffolds from 0.299 to 0.687 MPa.
33593130	1	26	theme	bredigite	190:198	arg1	bioceramics					233:243	bredigite (Br) and titanium dioxide (TiO2) bioceramics	190:243	bredigite (Br) and titanium dioxide (TiO2) bioceramics	190:243	In this study by considering the advantages of bredigite (Br) and titanium dioxide (TiO2) bioceramics, composite scaffolds of bredigite/titanium dioxide were prepared by the gelcasting method, then, to improve the mechanical, biological and antibacterial properties, scaffolds were coated with chitosan (Ch) polymer phase.
33593130	0	27	theme	scaffolds	132:140	arg1	properties					81:90	antibacterial and biocompatibility properties	46:90	antibacterial and biocompatibility properties of bredigite/titanium dioxide composite scaffolds	46:140	Investigation of applying chitosan coating on antibacterial and biocompatibility properties of bredigite/titanium dioxide composite scaffolds.
33593130	3	28	theme	porosity	688:695	arg1	percentage					697:706	porosity percentage	688:706	porosity percentage of scaffolds	688:719	Mechanical properties and porosity percentage of scaffolds were also measured by the compressive strength test and Archimedean method, respectively.
33593130	1	29	theme	polymer	451:457	arg1	phase					459:463	chitosan (Ch) polymer phase	437:463	chitosan (Ch) polymer phase	437:463	In this study by considering the advantages of bredigite (Br) and titanium dioxide (TiO2) bioceramics, composite scaffolds of bredigite/titanium dioxide were prepared by the gelcasting method, then, to improve the mechanical, biological and antibacterial properties, scaffolds were coated with chitosan (Ch) polymer phase.
33593130	7	30	theme	inhibition	1384:1393	arg1	zone					1395:1398	the inhibition zone	1380:1398	the inhibition zone	1380:1398	The results of the antibacterial test showed that in composite scaffolds, The diameter of the inhibition zone is 22 and 29 mm, in the culture media of Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive), respectively.
33593130	5	31	theme	compressive	1059:1069	arg1	strength					1071:1078	compressive strength	1059:1078	compressive strength of scaffolds from 0.299 to 0.687 MPa	1059:1115	The results showed that the addition of titanium dioxide to the scaffold of bredigite resulted in decrease of porosity and increase of compressive strength of scaffolds from 0.299 to 0.687 MPa.
33593130	5	32	from	strength	1071:1078	arg1	0.687 MPa					1107:1115	0.687 MPa	1107:1115	0.687 MPa	1107:1115	The results showed that the addition of titanium dioxide to the scaffold of bredigite resulted in decrease of porosity and increase of compressive strength of scaffolds from 0.299 to 0.687 MPa.
33593130	1	33	theme	biological	369:378	arg1	properties					398:407	the mechanical, biological and antibacterial properties	353:407	the mechanical, biological and antibacterial properties	353:407	In this study by considering the advantages of bredigite (Br) and titanium dioxide (TiO2) bioceramics, composite scaffolds of bredigite/titanium dioxide were prepared by the gelcasting method, then, to improve the mechanical, biological and antibacterial properties, scaffolds were coated with chitosan (Ch) polymer phase.
33593130	4	34	theme	cell	834:837	arg1	compatibility					839:851	the cell compatibility	830:851	the cell compatibility	830:851	In order to verify the cell compatibility, MG63 bone marrow cells were cultured on the surface of the specimens.
33593130	4	35	theme	specimens	913:921	arg1	surface					898:904	the surface	894:904	the surface of the specimens	894:921	In order to verify the cell compatibility, MG63 bone marrow cells were cultured on the surface of the specimens.
33593130	3	36	theme	scaffolds	711:719	arg1	properties					673:682	Mechanical properties	662:682	Mechanical properties	662:682	Mechanical properties and porosity percentage of scaffolds were also measured by the compressive strength test and Archimedean method, respectively.
33593130	3	36	theme	scaffolds	711:719	arg1	percentage					697:706	porosity percentage	688:706	porosity percentage of scaffolds	688:719	Mechanical properties and porosity percentage of scaffolds were also measured by the compressive strength test and Archimedean method, respectively.
33593130	1	37	theme	dioxide	288:294	arg1	scaffolds					256:264	composite scaffolds	246:264	composite scaffolds of bredigite/titanium dioxide	246:294	In this study by considering the advantages of bredigite (Br) and titanium dioxide (TiO2) bioceramics, composite scaffolds of bredigite/titanium dioxide were prepared by the gelcasting method, then, to improve the mechanical, biological and antibacterial properties, scaffolds were coated with chitosan (Ch) polymer phase.
33593130	7	38	theme	coli	1453:1456	arg1	media					1432:1436	the culture media	1420:1436	the culture media of Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive), respectively	1420:1528	The results of the antibacterial test showed that in composite scaffolds, The diameter of the inhibition zone is 22 and 29 mm, in the culture media of Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive), respectively.
33593130	7	39	dep	coli	1453:1456	arg1	Gram-negative					1459:1471	Gram-negative	1459:1471	Gram-negative	1459:1471	The results of the antibacterial test showed that in composite scaffolds, The diameter of the inhibition zone is 22 and 29 mm, in the culture media of Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive), respectively.
33593130	5	40	theme	porosity	1034:1041	arg1	decrease					1022:1029	decrease	1022:1029	decrease of porosity	1022:1041	The results showed that the addition of titanium dioxide to the scaffold of bredigite resulted in decrease of porosity and increase of compressive strength of scaffolds from 0.299 to 0.687 MPa.
33593130	5	40	theme	porosity	1034:1041	arg1	increase					1047:1054	increase	1047:1054	increase of compressive strength of scaffolds from 0.299 to 0.687 MPa	1047:1115	The results showed that the addition of titanium dioxide to the scaffold of bredigite resulted in decrease of porosity and increase of compressive strength of scaffolds from 0.299 to 0.687 MPa.
33593130	9	41	theme	ideal	1810:1814	arg1	scaffold					1769:1776	the scaffold	1765:1776	the scaffold in this study	1765:1790	Therefore, the scaffold in this study can be used as an ideal scaffold for use in bone tissue engineering.
33593130	9	41	theme	ideal	1810:1814	arg1	scaffold					1816:1823	an ideal scaffold	1807:1823	an ideal scaffold for use in bone tissue engineering	1807:1858	Therefore, the scaffold in this study can be used as an ideal scaffold for use in bone tissue engineering.
33593130	2	42	theme	distribution	543:554	arg1	analysis					556:563	elemental distribution analysis	533:563	elemental distribution analysis	533:563	The phase structure, fundamental groups, chemical composition, and elemental distribution analysis, morphology and the form of porosity were respectively characterized by XRD, FTIR, EDS, and SEM.
33593130	7	43	theme	antibacterial	1309:1321	arg1	test					1323:1326	the antibacterial test	1305:1326	the antibacterial test	1305:1326	The results of the antibacterial test showed that in composite scaffolds, The diameter of the inhibition zone is 22 and 29 mm, in the culture media of Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive), respectively.
33593130	9	44	from	scaffold	1769:1776	arg1	study					1786:1790	this study	1781:1790	this study	1781:1790	Therefore, the scaffold in this study can be used as an ideal scaffold for use in bone tissue engineering.
33593130	9	45	from	use	1829:1831	arg1	engineering					1848:1858	bone tissue engineering	1836:1858	bone tissue engineering	1836:1858	Therefore, the scaffold in this study can be used as an ideal scaffold for use in bone tissue engineering.
33593130	6	46	from	0.585	1270:1274	arg1	porosity					1181:1188	porosity	1181:1188	porosity from 83 to 63 percent	1181:1210	Furthermore, the coated scaffold with chitosan polymer reduced porosity from 83 to 63 percent and a remarkable improvement in compressive strength from 0.585 to 2.339 MPa.
33593130	6	46	from	0.585	1270:1274	arg1	improvement					1229:1239	a remarkable improvement	1216:1239	a remarkable improvement in compressive strength from 0.585 to 2.339 MPa	1216:1287	Furthermore, the coated scaffold with chitosan polymer reduced porosity from 83 to 63 percent and a remarkable improvement in compressive strength from 0.585 to 2.339 MPa.
33593130	6	46	from	0.585	1270:1274	arg1	strength					1256:1263	compressive strength	1244:1263	compressive strength from 0.585	1244:1274	Furthermore, the coated scaffold with chitosan polymer reduced porosity from 83 to 63 percent and a remarkable improvement in compressive strength from 0.585 to 2.339 MPa.
33593130	2	47	theme	elemental	533:541	arg1	analysis					556:563	elemental distribution analysis	533:563	elemental distribution analysis	533:563	The phase structure, fundamental groups, chemical composition, and elemental distribution analysis, morphology and the form of porosity were respectively characterized by XRD, FTIR, EDS, and SEM.
33593130	1	48	theme	titanium	209:216	arg1	TiO2					227:230	TiO2	227:230	TiO2	227:230	In this study by considering the advantages of bredigite (Br) and titanium dioxide (TiO2) bioceramics, composite scaffolds of bredigite/titanium dioxide were prepared by the gelcasting method, then, to improve the mechanical, biological and antibacterial properties, scaffolds were coated with chitosan (Ch) polymer phase.
33593130	1	48	theme	titanium	209:216	arg1	dioxide					218:224	titanium dioxide	209:224	titanium dioxide (TiO2)	209:231	In this study by considering the advantages of bredigite (Br) and titanium dioxide (TiO2) bioceramics, composite scaffolds of bredigite/titanium dioxide were prepared by the gelcasting method, then, to improve the mechanical, biological and antibacterial properties, scaffolds were coated with chitosan (Ch) polymer phase.
33593130	5	49	theme	dioxide	973:979	arg1	addition					952:959	the addition	948:959	the addition of titanium dioxide to the scaffold of bredigite	948:1008	The results showed that the addition of titanium dioxide to the scaffold of bredigite resulted in decrease of porosity and increase of compressive strength of scaffolds from 0.299 to 0.687 MPa.
33593130	1	50	theme	antibacterial	384:396	arg1	properties					398:407	the mechanical, biological and antibacterial properties	353:407	the mechanical, biological and antibacterial properties	353:407	In this study by considering the advantages of bredigite (Br) and titanium dioxide (TiO2) bioceramics, composite scaffolds of bredigite/titanium dioxide were prepared by the gelcasting method, then, to improve the mechanical, biological and antibacterial properties, scaffolds were coated with chitosan (Ch) polymer phase.
33593130	7	51	theme	composite	1343:1351	arg1	scaffolds					1353:1361	composite scaffolds	1343:1361	composite scaffolds	1343:1361	The results of the antibacterial test showed that in composite scaffolds, The diameter of the inhibition zone is 22 and 29 mm, in the culture media of Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive), respectively.
33593130	6	52	theme	chitosan	1156:1163	arg1	polymer					1165:1171	chitosan polymer	1156:1171	chitosan polymer	1156:1171	Furthermore, the coated scaffold with chitosan polymer reduced porosity from 83 to 63 percent and a remarkable improvement in compressive strength from 0.585 to 2.339 MPa.
33593130	1	53	theme	dioxide	218:224	arg1	bioceramics					233:243	bredigite (Br) and titanium dioxide (TiO2) bioceramics	190:243	bredigite (Br) and titanium dioxide (TiO2) bioceramics	190:243	In this study by considering the advantages of bredigite (Br) and titanium dioxide (TiO2) bioceramics, composite scaffolds of bredigite/titanium dioxide were prepared by the gelcasting method, then, to improve the mechanical, biological and antibacterial properties, scaffolds were coated with chitosan (Ch) polymer phase.
33593130	3	54	theme	strength	759:766	arg1	test					768:771	the compressive strength test and Archimedean method	743:794	test	768:771	Mechanical properties and porosity percentage of scaffolds were also measured by the compressive strength test and Archimedean method, respectively.
33593130	9	55	theme	bone	1836:1839	arg1	engineering					1848:1858	bone tissue engineering	1836:1858	bone tissue engineering	1836:1858	Therefore, the scaffold in this study can be used as an ideal scaffold for use in bone tissue engineering.
33593130	9	56	used	used	1799:1802	arg2	scaffold					1816:1823	an ideal scaffold	1807:1823	an ideal scaffold for use in bone tissue engineering	1807:1858	Therefore, the scaffold in this study can be used as an ideal scaffold for use in bone tissue engineering.
33593130	9	56	used	used	1799:1802	arg2	scaffold					1769:1776	the scaffold	1765:1776	the scaffold in this study	1765:1790	Therefore, the scaffold in this study can be used as an ideal scaffold for use in bone tissue engineering.
33593130	1	57	theme	gelcasting	317:326	arg1	method					328:333	the gelcasting method	313:333	the gelcasting method	313:333	In this study by considering the advantages of bredigite (Br) and titanium dioxide (TiO2) bioceramics, composite scaffolds of bredigite/titanium dioxide were prepared by the gelcasting method, then, to improve the mechanical, biological and antibacterial properties, scaffolds were coated with chitosan (Ch) polymer phase.
33593130	5	58	theme	strength	1071:1078	arg1	decrease					1022:1029	decrease	1022:1029	decrease of porosity	1022:1041	The results showed that the addition of titanium dioxide to the scaffold of bredigite resulted in decrease of porosity and increase of compressive strength of scaffolds from 0.299 to 0.687 MPa.
33593130	5	58	theme	strength	1071:1078	arg1	increase					1047:1054	increase	1047:1054	increase of compressive strength of scaffolds from 0.299 to 0.687 MPa	1047:1115	The results showed that the addition of titanium dioxide to the scaffold of bredigite resulted in decrease of porosity and increase of compressive strength of scaffolds from 0.299 to 0.687 MPa.
33593130	0	59	theme	antibacterial	46:58	arg1	properties					81:90	antibacterial and biocompatibility properties	46:90	antibacterial and biocompatibility properties of bredigite/titanium dioxide composite scaffolds	46:140	Investigation of applying chitosan coating on antibacterial and biocompatibility properties of bredigite/titanium dioxide composite scaffolds.
33593130	4	60	theme	marrow	864:869	arg1	cells					871:875	MG63 bone marrow cells	854:875	MG63 bone marrow cells	854:875	In order to verify the cell compatibility, MG63 bone marrow cells were cultured on the surface of the specimens.
33593130	5	61	theme	bredigite	1000:1008	arg1	scaffold					988:995	the scaffold	984:995	the scaffold of bredigite	984:1008	The results showed that the addition of titanium dioxide to the scaffold of bredigite resulted in decrease of porosity and increase of compressive strength of scaffolds from 0.299 to 0.687 MPa.
33593130	5	62	from	0.687 MPa	1107:1115	arg1	strength					1071:1078	compressive strength	1059:1078	compressive strength of scaffolds from 0.299 to 0.687 MPa	1059:1115	The results showed that the addition of titanium dioxide to the scaffold of bredigite resulted in decrease of porosity and increase of compressive strength of scaffolds from 0.299 to 0.687 MPa.
33593130	5	62	from	0.687 MPa	1107:1115	arg1	scaffolds					1083:1091	scaffolds	1083:1091	scaffolds from 0.299 to 0.687 MPa	1083:1115	The results showed that the addition of titanium dioxide to the scaffold of bredigite resulted in decrease of porosity and increase of compressive strength of scaffolds from 0.299 to 0.687 MPa.
33593130	3	63	theme	compressive	747:757	arg1	test					768:771	the compressive strength test and Archimedean method	743:794	test	768:771	Mechanical properties and porosity percentage of scaffolds were also measured by the compressive strength test and Archimedean method, respectively.
33593130	6	64	with	scaffold	1142:1149	arg1	polymer					1165:1171	chitosan polymer	1156:1171	chitosan polymer	1156:1171	Furthermore, the coated scaffold with chitosan polymer reduced porosity from 83 to 63 percent and a remarkable improvement in compressive strength from 0.585 to 2.339 MPa.
33593130	7	65	theme	test	1323:1326	arg1	results					1294:1300	The results	1290:1300	The results of the antibacterial test	1290:1326	The results of the antibacterial test showed that in composite scaffolds, The diameter of the inhibition zone is 22 and 29 mm, in the culture media of Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive), respectively.
33593130	6	66	dep	porosity	1181:1188	arg1	percent					1204:1210	from 83 to 63 percent	1190:1210	porosity from 83 to 63 percent	1181:1210	Furthermore, the coated scaffold with chitosan polymer reduced porosity from 83 to 63 percent and a remarkable improvement in compressive strength from 0.585 to 2.339 MPa.
33593130	8	67	theme	compatibility	1570:1582	arg1	tests					1602:1606	cell compatibility and cell adhesion tests	1565:1606	cell compatibility and cell adhesion tests	1565:1606	On the other hand, the results of cell compatibility and cell adhesion tests showed that the scaffolds had no toxicity and the growth, proliferation, and adhesion of MG63 bone cells adjacent to the scaffolds was desirable.
33593130	8	68	theme	other	1538:1542	arg1	hand					1544:1547	the other hand	1534:1547	the other hand	1534:1547	On the other hand, the results of cell compatibility and cell adhesion tests showed that the scaffolds had no toxicity and the growth, proliferation, and adhesion of MG63 bone cells adjacent to the scaffolds was desirable.
33593130	8	69	theme	cell	1588:1591	arg1	adhesion					1593:1600	cell adhesion	1588:1600	cell adhesion	1588:1600	On the other hand, the results of cell compatibility and cell adhesion tests showed that the scaffolds had no toxicity and the growth, proliferation, and adhesion of MG63 bone cells adjacent to the scaffolds was desirable.
33593130	7	70	theme	zone	1395:1398	arg1	diameter					1368:1375	The diameter	1364:1375	The diameter of the inhibition zone	1364:1398	The results of the antibacterial test showed that in composite scaffolds, The diameter of the inhibition zone is 22 and 29 mm, in the culture media of Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive), respectively.
33593130	7	70	theme	zone	1395:1398	arg1	22					1403:1404	22	1403:1404	22	1403:1404	The results of the antibacterial test showed that in composite scaffolds, The diameter of the inhibition zone is 22 and 29 mm, in the culture media of Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive), respectively.
33593130	8	71	theme	MG63	1697:1700	arg1	cells					1707:1711	MG63 bone cells	1697:1711	MG63 bone cells	1697:1711	On the other hand, the results of cell compatibility and cell adhesion tests showed that the scaffolds had no toxicity and the growth, proliferation, and adhesion of MG63 bone cells adjacent to the scaffolds was desirable.
33593130	8	72	theme	adhesion	1593:1600	arg1	tests					1602:1606	cell compatibility and cell adhesion tests	1565:1606	cell compatibility and cell adhesion tests	1565:1606	On the other hand, the results of cell compatibility and cell adhesion tests showed that the scaffolds had no toxicity and the growth, proliferation, and adhesion of MG63 bone cells adjacent to the scaffolds was desirable.
33593130	6	73	from	improvement	1229:1239	arg1	0.585					1270:1274	0.585	1270:1274	0.585	1270:1274	Furthermore, the coated scaffold with chitosan polymer reduced porosity from 83 to 63 percent and a remarkable improvement in compressive strength from 0.585 to 2.339 MPa.
33593130	6	73	from	improvement	1229:1239	arg1	strength					1256:1263	compressive strength	1244:1263	compressive strength from 0.585	1244:1274	Furthermore, the coated scaffold with chitosan polymer reduced porosity from 83 to 63 percent and a remarkable improvement in compressive strength from 0.585 to 2.339 MPa.
33593130	2	74	theme	fundamental	487:497	arg1	porosity					593:600	porosity	593:600	porosity	593:600	The phase structure, fundamental groups, chemical composition, and elemental distribution analysis, morphology and the form of porosity were respectively characterized by XRD, FTIR, EDS, and SEM.
33593130	2	74	theme	fundamental	487:497	arg1	groups					499:504	fundamental groups	487:504	fundamental groups	487:504	The phase structure, fundamental groups, chemical composition, and elemental distribution analysis, morphology and the form of porosity were respectively characterized by XRD, FTIR, EDS, and SEM.
33593130	5	75	theme	titanium	964:971	arg1	dioxide					973:979	titanium dioxide	964:979	titanium dioxide	964:979	The results showed that the addition of titanium dioxide to the scaffold of bredigite resulted in decrease of porosity and increase of compressive strength of scaffolds from 0.299 to 0.687 MPa.
33593130	6	76	theme	coated	1135:1140	arg1	scaffold					1142:1149	the coated scaffold	1131:1149	the coated scaffold with chitosan polymer	1131:1171	Furthermore, the coated scaffold with chitosan polymer reduced porosity from 83 to 63 percent and a remarkable improvement in compressive strength from 0.585 to 2.339 MPa.
33593130	1	77	theme	bioceramics	233:243	arg1	advantages					176:185	the advantages	172:185	the advantages of bredigite (Br) and titanium dioxide (TiO2) bioceramics	172:243	In this study by considering the advantages of bredigite (Br) and titanium dioxide (TiO2) bioceramics, composite scaffolds of bredigite/titanium dioxide were prepared by the gelcasting method, then, to improve the mechanical, biological and antibacterial properties, scaffolds were coated with chitosan (Ch) polymer phase.
33593130	0	78	theme	biocompatibility	64:79	arg1	properties					81:90	antibacterial and biocompatibility properties	46:90	antibacterial and biocompatibility properties of bredigite/titanium dioxide composite scaffolds	46:140	Investigation of applying chitosan coating on antibacterial and biocompatibility properties of bredigite/titanium dioxide composite scaffolds.
33593130	5	79	theme	scaffolds	1083:1091	arg1	strength					1071:1078	compressive strength	1059:1078	compressive strength of scaffolds from 0.299 to 0.687 MPa	1059:1115	The results showed that the addition of titanium dioxide to the scaffold of bredigite resulted in decrease of porosity and increase of compressive strength of scaffolds from 0.299 to 0.687 MPa.
33593130	3	80	theme	Archimedean	777:787	arg1	method					789:794	the compressive strength test and Archimedean method	743:794	method	789:794	Mechanical properties and porosity percentage of scaffolds were also measured by the compressive strength test and Archimedean method, respectively.
33593130	8	81	theme	cells	1707:1711	arg1	proliferation					1666:1678	proliferation	1666:1678	proliferation	1666:1678	On the other hand, the results of cell compatibility and cell adhesion tests showed that the scaffolds had no toxicity and the growth, proliferation, and adhesion of MG63 bone cells adjacent to the scaffolds was desirable.
33593130	8	81	theme	cells	1707:1711	arg1	toxicity					1641:1648	toxicity	1641:1648	toxicity	1641:1648	On the other hand, the results of cell compatibility and cell adhesion tests showed that the scaffolds had no toxicity and the growth, proliferation, and adhesion of MG63 bone cells adjacent to the scaffolds was desirable.
33593130	8	81	theme	cells	1707:1711	arg1	growth					1658:1663	growth	1658:1663	growth	1658:1663	On the other hand, the results of cell compatibility and cell adhesion tests showed that the scaffolds had no toxicity and the growth, proliferation, and adhesion of MG63 bone cells adjacent to the scaffolds was desirable.
33593130	8	81	theme	cells	1707:1711	arg1	adhesion					1685:1692	adhesion	1685:1692	adhesion	1685:1692	On the other hand, the results of cell compatibility and cell adhesion tests showed that the scaffolds had no toxicity and the growth, proliferation, and adhesion of MG63 bone cells adjacent to the scaffolds was desirable.
33593130	7	82	from	22	1403:1404	arg1	media					1432:1436	the culture media	1420:1436	the culture media of Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive), respectively	1420:1528	The results of the antibacterial test showed that in composite scaffolds, The diameter of the inhibition zone is 22 and 29 mm, in the culture media of Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive), respectively.
33593130	7	82	from	22	1403:1404	arg1	scaffolds					1353:1361	composite scaffolds	1343:1361	composite scaffolds	1343:1361	The results of the antibacterial test showed that in composite scaffolds, The diameter of the inhibition zone is 22 and 29 mm, in the culture media of Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive), respectively.
33593130	8	83	theme	cell	1565:1568	arg1	compatibility					1570:1582	cell compatibility	1565:1582	cell compatibility	1565:1582	On the other hand, the results of cell compatibility and cell adhesion tests showed that the scaffolds had no toxicity and the growth, proliferation, and adhesion of MG63 bone cells adjacent to the scaffolds was desirable.
33593130	2	84	theme	chemical	507:514	arg1	composition					516:526	chemical composition	507:526	chemical composition	507:526	The phase structure, fundamental groups, chemical composition, and elemental distribution analysis, morphology and the form of porosity were respectively characterized by XRD, FTIR, EDS, and SEM.
32840994	8	0	theme	photoelectron	1539:1551	arg1	XPS					1567:1569	XPS	1567:1569	XPS	1567:1569	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	8	0	theme	photoelectron	1539:1551	arg1	spectroscopy					1553:1564	X-ray photoelectron spectroscopy	1533:1564	X-ray photoelectron spectroscopy (XPS)	1533:1570	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	9	1	theme	presented	1577:1585	arg1	approach					1587:1594	The presented approach	1573:1594	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system)	1573:1761	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system) enables the significant enhancement of materials functionality.
32840994	6	2	theme	aqueous	1062:1068	arg1	solutions					1070:1078	diluted aqueous solutions	1054:1078	diluted aqueous solutions	1054:1078	CM/Bi4O5Br2/BiOBr showed higher affinity to Co(II) ions than to Ni(II) ions from diluted aqueous solutions.
32840994	9	3	theme	morphology	1735:1744	arg1	materials					1713:1721	nanoscopic inorganic materials	1692:1721	nanoscopic inorganic materials of tailored morphology (Bi-O-Br system)	1692:1761	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system) enables the significant enhancement of materials functionality.
32840994	9	3	theme	morphology	1735:1744	arg1	cellulose					1677:1685	cellulose	1677:1685	cellulose	1677:1685	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system) enables the significant enhancement of materials functionality.
32840994	9	3	theme	morphology	1735:1744	arg1	system					1755:1760	Bi-O-Br system	1747:1760	Bi-O-Br system	1747:1760	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system) enables the significant enhancement of materials functionality.
32840994	9	3	theme	morphology	1735:1744	arg1	material					1667:1674	natural material	1659:1674	natural material (cellulose)	1659:1686	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system) enables the significant enhancement of materials functionality.
32840994	2	4	theme	material	279:286	arg1	fabrication					255:265	the fabrication	251:265	the fabrication of a hybrid material that can fulfill both tasks: (i) the adsorption of metal ions due to the negative surface charge, and (ii) photocatalytic decomposition of organic compounds	251:443	Here, we demonstrate the fabrication of a hybrid material that can fulfill both tasks: (i) the adsorption of metal ions due to the negative surface charge, and (ii) photocatalytic decomposition of organic compounds.
32840994	8	5	with	spectroscopy	1553:1564	arg1	mapping					1495:1501	energy dispersive X-ray spectroscopy (STEM EDX) mapping	1447:1501	energy dispersive X-ray spectroscopy (STEM EDX) mapping	1447:1501	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	1	6	theme	several	200:206	arg1	processes					219:227	usually several sequential processes	192:227	usually several sequential processes	192:227	The simultaneous removal of organic and inorganic pollutants from wastewater is a complex challenge and requires usually several sequential processes.
32840994	8	7	theme	electron	1373:1380	arg1	SEM					1394:1396	SEM	1394:1396	SEM	1394:1396	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	8	7	theme	electron	1373:1380	arg1	microscopy					1382:1391	scanning electron microscopy	1364:1391	scanning electron microscopy (SEM)	1364:1397	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	5	8	with	agreement	889:897	arg1	model					966:970	the pseudo-second-order equation kinetic model	925:970	the pseudo-second-order equation kinetic model	925:970	The composite can adsorb both Co(II) and Ni(II) ions and the kinetic study confirmed a good agreement of experimental data with the pseudo-second-order equation kinetic model.
32840994	9	9	theme	interface	1641:1649	arg1	formation					1611:1619	controlled formation	1600:1619	controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system)	1600:1761	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system) enables the significant enhancement of materials functionality.
32840994	1	10	theme	pollutants	129:138	arg1	removal					96:102	The simultaneous removal	79:102	The simultaneous removal of organic and inorganic pollutants from wastewater	79:154	The simultaneous removal of organic and inorganic pollutants from wastewater is a complex challenge and requires usually several sequential processes.
32840994	1	10	theme	pollutants	129:138	arg1	challenge					169:177	a complex challenge	159:177	a complex challenge	159:177	The simultaneous removal of organic and inorganic pollutants from wastewater is a complex challenge and requires usually several sequential processes.
32840994	9	11	theme	natural	1659:1665	arg1	cellulose					1677:1685	cellulose	1677:1685	cellulose	1677:1685	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system) enables the significant enhancement of materials functionality.
32840994	9	11	theme	natural	1659:1665	arg1	material					1667:1674	natural material	1659:1674	natural material (cellulose)	1659:1686	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system) enables the significant enhancement of materials functionality.
32840994	6	12	dep	Co	1017:1018	arg1	ions					1024:1027	ions	1024:1027	Co(II) ions	1017:1027	CM/Bi4O5Br2/BiOBr showed higher affinity to Co(II) ions than to Ni(II) ions from diluted aqueous solutions.
32840994	6	12	dep	Co	1017:1018	arg1	II					1020:1021	II	1020:1021	II	1020:1021	CM/Bi4O5Br2/BiOBr showed higher affinity to Co(II) ions than to Ni(II) ions from diluted aqueous solutions.
32840994	8	13	with	diffraction	1510:1520	arg1	mapping					1495:1501	energy dispersive X-ray spectroscopy (STEM EDX) mapping	1447:1501	energy dispersive X-ray spectroscopy (STEM EDX) mapping	1447:1501	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	8	14	theme	scanning	1400:1407	arg1	microscopy					1431:1440	scanning transmission electron microscopy	1400:1440	scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping	1400:1501	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	7	15	theme	components	1243:1252	arg1	efficiency					1222:1231	the removal efficiency	1210:1231	the removal efficiency of single components	1210:1252	The bioinorganic composite demonstrates a synergistic effect in the photocatalytic degradation of rhodamine B (RhB) by exceeding the removal efficiency of single components.
32840994	8	16	theme	electron	1422:1429	arg1	microscopy					1431:1440	scanning transmission electron microscopy	1400:1440	scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping	1400:1501	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	7	17	theme	synergistic	1123:1133	arg1	effect					1135:1140	a synergistic effect	1121:1140	a synergistic effect	1121:1140	The bioinorganic composite demonstrates a synergistic effect in the photocatalytic degradation of rhodamine B (RhB) by exceeding the removal efficiency of single components.
32840994	9	18	theme	nanoscopic	1692:1701	arg1	materials					1713:1721	nanoscopic inorganic materials	1692:1721	nanoscopic inorganic materials of tailored morphology (Bi-O-Br system)	1692:1761	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system) enables the significant enhancement of materials functionality.
32840994	9	18	theme	nanoscopic	1692:1701	arg1	system					1755:1760	Bi-O-Br system	1747:1760	Bi-O-Br system	1747:1760	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system) enables the significant enhancement of materials functionality.
32840994	4	19	theme	hydroxyl	711:718	arg1	groups					720:725	the carboxylic and hydroxyl groups	692:725	groups	720:725	The composite is synthesized at low temperature of 115 °C directly on the cellulose membrane (CM) in order to maintain the carboxylic and hydroxyl groups on the surface that are responsible for the adsorption of metal ions.
32840994	8	20	theme	dispersive	1454:1463	arg1	spectroscopy					1471:1482	energy dispersive X-ray spectroscopy	1447:1482	energy dispersive X-ray spectroscopy (STEM EDX) mapping	1447:1501	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	8	20	theme	dispersive	1454:1463	arg1	EDX					1490:1492	STEM EDX	1485:1492	STEM EDX	1485:1492	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	2	21	theme	surface	370:376	arg1	charge					378:383	the negative surface charge	357:383	the negative surface charge	357:383	Here, we demonstrate the fabrication of a hybrid material that can fulfill both tasks: (i) the adsorption of metal ions due to the negative surface charge, and (ii) photocatalytic decomposition of organic compounds.
32840994	7	22	theme	bioinorganic	1085:1096	arg1	composite					1098:1106	The bioinorganic composite	1081:1106	The bioinorganic composite	1081:1106	The bioinorganic composite demonstrates a synergistic effect in the photocatalytic degradation of rhodamine B (RhB) by exceeding the removal efficiency of single components.
32840994	4	23	from	groups	720:725	arg1	surface					734:740	the surface	730:740	the surface that are responsible for the adsorption of metal ions	730:794	The composite is synthesized at low temperature of 115 °C directly on the cellulose membrane (CM) in order to maintain the carboxylic and hydroxyl groups on the surface that are responsible for the adsorption of metal ions.
32840994	4	23	from	groups	720:725	arg1	responsible					751:761	responsible	751:761	responsible	751:761	The composite is synthesized at low temperature of 115 °C directly on the cellulose membrane (CM) in order to maintain the carboxylic and hydroxyl groups on the surface that are responsible for the adsorption of metal ions.
32840994	1	24	theme	simultaneous	83:94	arg1	removal					96:102	The simultaneous removal	79:102	The simultaneous removal of organic and inorganic pollutants from wastewater	79:154	The simultaneous removal of organic and inorganic pollutants from wastewater is a complex challenge and requires usually several sequential processes.
32840994	1	24	theme	simultaneous	83:94	arg1	challenge					169:177	a complex challenge	159:177	a complex challenge	159:177	The simultaneous removal of organic and inorganic pollutants from wastewater is a complex challenge and requires usually several sequential processes.
32840994	7	25	theme	single	1236:1241	arg1	components					1243:1252	single components	1236:1252	single components	1236:1252	The bioinorganic composite demonstrates a synergistic effect in the photocatalytic degradation of rhodamine B (RhB) by exceeding the removal efficiency of single components.
32840994	9	26	theme	tailored	1726:1733	arg1	morphology					1735:1744	tailored morphology	1726:1744	tailored morphology	1726:1744	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system) enables the significant enhancement of materials functionality.
32840994	8	27	theme	spectroscopy	1471:1482	arg1	mapping					1495:1501	energy dispersive X-ray spectroscopy (STEM EDX) mapping	1447:1501	energy dispersive X-ray spectroscopy (STEM EDX) mapping	1447:1501	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	6	28	from	solutions	1070:1078	arg1	ions					1044:1047	Ni(II) ions	1037:1047	Ni(II) ions from diluted aqueous solutions	1037:1078	CM/Bi4O5Br2/BiOBr showed higher affinity to Co(II) ions than to Ni(II) ions from diluted aqueous solutions.
32840994	8	29	with	microscopy	1431:1440	arg1	mapping					1495:1501	energy dispersive X-ray spectroscopy (STEM EDX) mapping	1447:1501	energy dispersive X-ray spectroscopy (STEM EDX) mapping	1447:1501	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	6	30	theme	higher	998:1003	arg1	affinity					1005:1012	higher affinity	998:1012	higher affinity to Co(II) ions than to Ni(II) ions from diluted aqueous solutions	998:1078	CM/Bi4O5Br2/BiOBr showed higher affinity to Co(II) ions than to Ni(II) ions from diluted aqueous solutions.
32840994	8	31	theme	STEM	1485:1488	arg1	spectroscopy					1471:1482	energy dispersive X-ray spectroscopy	1447:1482	energy dispersive X-ray spectroscopy (STEM EDX) mapping	1447:1501	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	8	31	theme	STEM	1485:1488	arg1	EDX					1490:1492	STEM EDX	1485:1492	STEM EDX	1485:1492	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	8	32	theme	interface	1297:1305	arg1	fabrication					1259:1269	The fabrication	1255:1269	The fabrication of the biologic-inorganic interface	1255:1305	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	0	33	theme	Hybrid	11:16	arg1	Membrane					47:54	Hybrid Cellulose-Bismuth Oxybromide Membrane	11:54	CelluPhot: Hybrid Cellulose-Bismuth Oxybromide Membrane for Pollutant Removal.	0:77	CelluPhot: Hybrid Cellulose-Bismuth Oxybromide Membrane for Pollutant Removal.
32840994	3	34	dep	fibers	501:506	arg1	ensure					511:516	ensure	511:516	to ensure mechanical stability	508:537	The bioinorganic hybrid membrane consists of cellulose fibers to ensure mechanical stability and of Bi4O5Br2/BiOBr nanosheets.
32840994	5	35	dep	Co	827:828	arg1	ions					845:848	ions	845:848	ions	845:848	The composite can adsorb both Co(II) and Ni(II) ions and the kinetic study confirmed a good agreement of experimental data with the pseudo-second-order equation kinetic model.
32840994	0	36	theme	Oxybromide	36:45	arg1	Membrane					47:54	Hybrid Cellulose-Bismuth Oxybromide Membrane	11:54	CelluPhot: Hybrid Cellulose-Bismuth Oxybromide Membrane for Pollutant Removal.	0:77	CelluPhot: Hybrid Cellulose-Bismuth Oxybromide Membrane for Pollutant Removal.
32840994	5	37	theme	pseudo-second-order	929:947	arg1	model					966:970	the pseudo-second-order equation kinetic model	925:970	the pseudo-second-order equation kinetic model	925:970	The composite can adsorb both Co(II) and Ni(II) ions and the kinetic study confirmed a good agreement of experimental data with the pseudo-second-order equation kinetic model.
32840994	2	38	theme	organic	427:433	arg1	compounds					435:443	organic compounds	427:443	organic compounds	427:443	Here, we demonstrate the fabrication of a hybrid material that can fulfill both tasks: (i) the adsorption of metal ions due to the negative surface charge, and (ii) photocatalytic decomposition of organic compounds.
32840994	4	39	theme	ions	791:794	arg1	adsorption					771:780	the adsorption	767:780	the adsorption of metal ions	767:794	The composite is synthesized at low temperature of 115 °C directly on the cellulose membrane (CM) in order to maintain the carboxylic and hydroxyl groups on the surface that are responsible for the adsorption of metal ions.
32840994	3	40	theme	hybrid	463:468	arg1	membrane					470:477	The bioinorganic hybrid membrane	446:477	The bioinorganic hybrid membrane	446:477	The bioinorganic hybrid membrane consists of cellulose fibers to ensure mechanical stability and of Bi4O5Br2/BiOBr nanosheets.
32840994	5	41	theme	kinetic	858:864	arg1	study					866:870	the kinetic study	854:870	the kinetic study	854:870	The composite can adsorb both Co(II) and Ni(II) ions and the kinetic study confirmed a good agreement of experimental data with the pseudo-second-order equation kinetic model.
32840994	5	42	theme	kinetic	958:964	arg1	model					966:970	the pseudo-second-order equation kinetic model	925:970	the pseudo-second-order equation kinetic model	925:970	The composite can adsorb both Co(II) and Ni(II) ions and the kinetic study confirmed a good agreement of experimental data with the pseudo-second-order equation kinetic model.
32840994	6	43	theme	Ni	1037:1038	arg1	ions					1044:1047	Ni(II) ions	1037:1047	Ni(II) ions from diluted aqueous solutions	1037:1078	CM/Bi4O5Br2/BiOBr showed higher affinity to Co(II) ions than to Ni(II) ions from diluted aqueous solutions.
32840994	9	44	theme	significant	1775:1785	arg1	enhancement					1787:1797	the significant enhancement	1771:1797	the significant enhancement of materials functionality	1771:1824	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system) enables the significant enhancement of materials functionality.
32840994	8	45	with	microscopy	1382:1391	arg1	mapping					1495:1501	energy dispersive X-ray spectroscopy (STEM EDX) mapping	1447:1501	energy dispersive X-ray spectroscopy (STEM EDX) mapping	1447:1501	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	5	46	theme	experimental	902:913	arg1	data					915:918	experimental data	902:918	experimental data	902:918	The composite can adsorb both Co(II) and Ni(II) ions and the kinetic study confirmed a good agreement of experimental data with the pseudo-second-order equation kinetic model.
32840994	7	47	theme	B	1189:1189	arg1	degradation					1164:1174	the photocatalytic degradation	1145:1174	the photocatalytic degradation of rhodamine B (RhB)	1145:1195	The bioinorganic composite demonstrates a synergistic effect in the photocatalytic degradation of rhodamine B (RhB) by exceeding the removal efficiency of single components.
32840994	8	48	theme	scanning	1364:1371	arg1	SEM					1394:1396	SEM	1394:1396	SEM	1394:1396	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	8	48	theme	scanning	1364:1371	arg1	microscopy					1382:1391	scanning electron microscopy	1364:1391	scanning electron microscopy (SEM)	1364:1397	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	3	49	theme	cellulose	491:499	arg1	fibers					501:506	cellulose fibers	491:506	cellulose fibers to ensure mechanical stability	491:537	The bioinorganic hybrid membrane consists of cellulose fibers to ensure mechanical stability and of Bi4O5Br2/BiOBr nanosheets.
32840994	0	50	dep	CelluPhot	0:8	arg1	Membrane					47:54	Hybrid Cellulose-Bismuth Oxybromide Membrane	11:54	CelluPhot: Hybrid Cellulose-Bismuth Oxybromide Membrane for Pollutant Removal.	0:77	CelluPhot: Hybrid Cellulose-Bismuth Oxybromide Membrane for Pollutant Removal.
32840994	9	51	theme	functionality	1812:1824	arg1	enhancement					1787:1797	the significant enhancement	1771:1797	the significant enhancement of materials functionality	1771:1824	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system) enables the significant enhancement of materials functionality.
32840994	4	52	theme	low	605:607	arg1	temperature					609:619	low temperature	605:619	low temperature of 115 °C	605:629	The composite is synthesized at low temperature of 115 °C directly on the cellulose membrane (CM) in order to maintain the carboxylic and hydroxyl groups on the surface that are responsible for the adsorption of metal ions.
32840994	2	53	theme	metal	339:343	arg1	ions					345:348	metal ions	339:348	metal ions due to the negative surface charge	339:383	Here, we demonstrate the fabrication of a hybrid material that can fulfill both tasks: (i) the adsorption of metal ions due to the negative surface charge, and (ii) photocatalytic decomposition of organic compounds.
32840994	7	54	theme	photocatalytic	1149:1162	arg1	degradation					1164:1174	the photocatalytic degradation	1145:1174	the photocatalytic degradation of rhodamine B (RhB)	1145:1195	The bioinorganic composite demonstrates a synergistic effect in the photocatalytic degradation of rhodamine B (RhB) by exceeding the removal efficiency of single components.
32840994	8	55	theme	X-ray	1533:1537	arg1	XPS					1567:1569	XPS	1567:1569	XPS	1567:1569	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	8	55	theme	X-ray	1533:1537	arg1	spectroscopy					1553:1564	X-ray photoelectron spectroscopy	1533:1564	X-ray photoelectron spectroscopy (XPS)	1533:1570	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	8	56	theme	various	1324:1330	arg1	spectroscopy					1553:1564	X-ray photoelectron spectroscopy	1533:1564	X-ray photoelectron spectroscopy (XPS)	1533:1570	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	8	56	theme	various	1324:1330	arg1	techniques					1343:1352	various analytical techniques	1324:1352	various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS)	1324:1570	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	8	56	theme	various	1324:1330	arg1	diffraction					1510:1520	X-ray diffraction	1504:1520	X-ray diffraction (XRD)	1504:1526	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	8	56	theme	various	1324:1330	arg1	microscopy					1382:1391	scanning electron microscopy	1364:1391	scanning electron microscopy (SEM)	1364:1397	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	8	56	theme	various	1324:1330	arg1	microscopy					1431:1440	scanning transmission electron microscopy	1400:1440	scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping	1400:1501	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	3	57	theme	mechanical	518:527	arg1	stability					529:537	mechanical stability	518:537	mechanical stability	518:537	The bioinorganic hybrid membrane consists of cellulose fibers to ensure mechanical stability and of Bi4O5Br2/BiOBr nanosheets.
32840994	6	58	theme	diluted	1054:1060	arg1	solutions					1070:1078	diluted aqueous solutions	1054:1078	diluted aqueous solutions	1054:1078	CM/Bi4O5Br2/BiOBr showed higher affinity to Co(II) ions than to Ni(II) ions from diluted aqueous solutions.
32840994	3	59	theme	Bi4O5Br2/BiOBr	546:559	arg1	nanosheets					561:570	Bi4O5Br2/BiOBr nanosheets	546:570	Bi4O5Br2/BiOBr nanosheets	546:570	The bioinorganic hybrid membrane consists of cellulose fibers to ensure mechanical stability and of Bi4O5Br2/BiOBr nanosheets.
32840994	9	60	theme	controlled	1600:1609	arg1	formation					1611:1619	controlled formation	1600:1619	controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system)	1600:1761	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system) enables the significant enhancement of materials functionality.
32840994	4	61	theme	cellulose	647:655	arg1	CM					667:668	CM	667:668	CM	667:668	The composite is synthesized at low temperature of 115 °C directly on the cellulose membrane (CM) in order to maintain the carboxylic and hydroxyl groups on the surface that are responsible for the adsorption of metal ions.
32840994	4	61	theme	cellulose	647:655	arg1	membrane					657:664	the cellulose membrane	643:664	the cellulose membrane (CM)	643:669	The composite is synthesized at low temperature of 115 °C directly on the cellulose membrane (CM) in order to maintain the carboxylic and hydroxyl groups on the surface that are responsible for the adsorption of metal ions.
32840994	1	62	theme	sequential	208:217	arg1	processes					219:227	usually several sequential processes	192:227	usually several sequential processes	192:227	The simultaneous removal of organic and inorganic pollutants from wastewater is a complex challenge and requires usually several sequential processes.
32840994	9	63	theme	bioinorganic	1628:1639	arg1	interface					1641:1649	the bioinorganic interface	1624:1649	the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system)	1624:1761	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system) enables the significant enhancement of materials functionality.
32840994	4	64	theme	°C	628:629	arg1	temperature					609:619	low temperature	605:619	low temperature of 115 °C	605:629	The composite is synthesized at low temperature of 115 °C directly on the cellulose membrane (CM) in order to maintain the carboxylic and hydroxyl groups on the surface that are responsible for the adsorption of metal ions.
32840994	1	65	theme	inorganic	119:127	arg1	pollutants					129:138	organic and inorganic pollutants	107:138	organic and inorganic pollutants	107:138	The simultaneous removal of organic and inorganic pollutants from wastewater is a complex challenge and requires usually several sequential processes.
32840994	2	66	dep	adsorption	325:334	arg1	i					318:318	i	318:318	i	318:318	Here, we demonstrate the fabrication of a hybrid material that can fulfill both tasks: (i) the adsorption of metal ions due to the negative surface charge, and (ii) photocatalytic decomposition of organic compounds.
32840994	2	67	theme	hybrid	272:277	arg1	material					279:286	a hybrid material	270:286	a hybrid material that can fulfill both tasks: (i) the adsorption of metal ions due to the negative surface charge, and (ii) photocatalytic decomposition of organic compounds	270:443	Here, we demonstrate the fabrication of a hybrid material that can fulfill both tasks: (i) the adsorption of metal ions due to the negative surface charge, and (ii) photocatalytic decomposition of organic compounds.
32840994	8	68	theme	transmission	1409:1420	arg1	microscopy					1431:1440	scanning transmission electron microscopy	1400:1440	scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping	1400:1501	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	1	69	theme	complex	161:167	arg1	removal					96:102	The simultaneous removal	79:102	The simultaneous removal of organic and inorganic pollutants from wastewater	79:154	The simultaneous removal of organic and inorganic pollutants from wastewater is a complex challenge and requires usually several sequential processes.
32840994	1	69	theme	complex	161:167	arg1	challenge					169:177	a complex challenge	159:177	a complex challenge	159:177	The simultaneous removal of organic and inorganic pollutants from wastewater is a complex challenge and requires usually several sequential processes.
32840994	8	70	theme	energy	1447:1452	arg1	spectroscopy					1471:1482	energy dispersive X-ray spectroscopy	1447:1482	energy dispersive X-ray spectroscopy (STEM EDX) mapping	1447:1501	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	8	70	theme	energy	1447:1452	arg1	EDX					1490:1492	STEM EDX	1485:1492	STEM EDX	1485:1492	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	9	71	theme	inorganic	1703:1711	arg1	materials					1713:1721	nanoscopic inorganic materials	1692:1721	nanoscopic inorganic materials of tailored morphology (Bi-O-Br system)	1692:1761	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system) enables the significant enhancement of materials functionality.
32840994	9	71	theme	inorganic	1703:1711	arg1	system					1755:1760	Bi-O-Br system	1747:1760	Bi-O-Br system	1747:1760	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system) enables the significant enhancement of materials functionality.
32840994	8	72	theme	X-ray	1465:1469	arg1	spectroscopy					1471:1482	energy dispersive X-ray spectroscopy	1447:1482	energy dispersive X-ray spectroscopy (STEM EDX) mapping	1447:1501	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	8	72	theme	X-ray	1465:1469	arg1	EDX					1490:1492	STEM EDX	1485:1492	STEM EDX	1485:1492	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	5	73	theme	good	884:887	arg1	agreement					889:897	a good agreement	882:897	a good agreement of experimental data with the pseudo-second-order equation kinetic model	882:970	The composite can adsorb both Co(II) and Ni(II) ions and the kinetic study confirmed a good agreement of experimental data with the pseudo-second-order equation kinetic model.
32840994	2	74	theme	negative	361:368	arg1	charge					378:383	the negative surface charge	357:383	the negative surface charge	357:383	Here, we demonstrate the fabrication of a hybrid material that can fulfill both tasks: (i) the adsorption of metal ions due to the negative surface charge, and (ii) photocatalytic decomposition of organic compounds.
32840994	2	75	dep	decomposition	410:422	arg1	ii					391:392	ii	391:392	ii	391:392	Here, we demonstrate the fabrication of a hybrid material that can fulfill both tasks: (i) the adsorption of metal ions due to the negative surface charge, and (ii) photocatalytic decomposition of organic compounds.
32840994	1	76	from	wastewater	145:154	arg1	removal					96:102	The simultaneous removal	79:102	The simultaneous removal of organic and inorganic pollutants from wastewater	79:154	The simultaneous removal of organic and inorganic pollutants from wastewater is a complex challenge and requires usually several sequential processes.
32840994	1	76	from	wastewater	145:154	arg1	challenge					169:177	a complex challenge	159:177	a complex challenge	159:177	The simultaneous removal of organic and inorganic pollutants from wastewater is a complex challenge and requires usually several sequential processes.
32840994	7	77	theme	removal	1214:1220	arg1	efficiency					1222:1231	the removal efficiency	1210:1231	the removal efficiency of single components	1210:1252	The bioinorganic composite demonstrates a synergistic effect in the photocatalytic degradation of rhodamine B (RhB) by exceeding the removal efficiency of single components.
32840994	2	78	theme	photocatalytic	395:408	arg1	decomposition					410:422	photocatalytic decomposition	395:422	(ii) photocatalytic decomposition of organic compounds	390:443	Here, we demonstrate the fabrication of a hybrid material that can fulfill both tasks: (i) the adsorption of metal ions due to the negative surface charge, and (ii) photocatalytic decomposition of organic compounds.
32840994	4	79	theme	carboxylic	696:705	arg1	groups					720:725	the carboxylic and hydroxyl groups	692:725	groups	720:725	The composite is synthesized at low temperature of 115 °C directly on the cellulose membrane (CM) in order to maintain the carboxylic and hydroxyl groups on the surface that are responsible for the adsorption of metal ions.
32840994	0	80	theme	Cellulose-Bismuth	18:34	arg1	Membrane					47:54	Hybrid Cellulose-Bismuth Oxybromide Membrane	11:54	CelluPhot: Hybrid Cellulose-Bismuth Oxybromide Membrane for Pollutant Removal.	0:77	CelluPhot: Hybrid Cellulose-Bismuth Oxybromide Membrane for Pollutant Removal.
32840994	1	81	theme	organic	107:113	arg1	pollutants					129:138	organic and inorganic pollutants	107:138	organic and inorganic pollutants	107:138	The simultaneous removal of organic and inorganic pollutants from wastewater is a complex challenge and requires usually several sequential processes.
32840994	9	82	theme	Bi-O-Br	1747:1753	arg1	materials					1713:1721	nanoscopic inorganic materials	1692:1721	nanoscopic inorganic materials of tailored morphology (Bi-O-Br system)	1692:1761	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system) enables the significant enhancement of materials functionality.
32840994	9	82	theme	Bi-O-Br	1747:1753	arg1	system					1755:1760	Bi-O-Br system	1747:1760	Bi-O-Br system	1747:1760	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system) enables the significant enhancement of materials functionality.
32840994	8	83	theme	biologic-inorganic	1278:1295	arg1	interface					1297:1305	the biologic-inorganic interface	1274:1305	the biologic-inorganic interface	1274:1305	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	2	84	theme	due	350:352	arg1	ions					345:348	metal ions	339:348	metal ions due to the negative surface charge	339:383	Here, we demonstrate the fabrication of a hybrid material that can fulfill both tasks: (i) the adsorption of metal ions due to the negative surface charge, and (ii) photocatalytic decomposition of organic compounds.
32840994	2	85	theme	compounds	435:443	arg1	decomposition					410:422	photocatalytic decomposition	395:422	(ii) photocatalytic decomposition of organic compounds	390:443	Here, we demonstrate the fabrication of a hybrid material that can fulfill both tasks: (i) the adsorption of metal ions due to the negative surface charge, and (ii) photocatalytic decomposition of organic compounds.
32840994	2	85	theme	compounds	435:443	arg1	tasks					310:314	both tasks	305:314	both tasks	305:314	Here, we demonstrate the fabrication of a hybrid material that can fulfill both tasks: (i) the adsorption of metal ions due to the negative surface charge, and (ii) photocatalytic decomposition of organic compounds.
32840994	2	85	theme	compounds	435:443	arg1	adsorption					325:334	the adsorption	321:334	(i) the adsorption of metal ions due to the negative surface charge	317:383	Here, we demonstrate the fabrication of a hybrid material that can fulfill both tasks: (i) the adsorption of metal ions due to the negative surface charge, and (ii) photocatalytic decomposition of organic compounds.
32840994	8	86	theme	X-ray	1504:1508	arg1	XRD					1523:1525	XRD	1523:1525	XRD	1523:1525	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	8	86	theme	X-ray	1504:1508	arg1	diffraction					1510:1520	X-ray diffraction	1504:1520	X-ray diffraction (XRD)	1504:1526	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	0	87	theme	Pollutant	60:68	arg1	Removal					70:76	Pollutant Removal	60:76	Pollutant Removal	60:76	CelluPhot: Hybrid Cellulose-Bismuth Oxybromide Membrane for Pollutant Removal.
32840994	5	88	theme	equation	949:956	arg1	model					966:970	the pseudo-second-order equation kinetic model	925:970	the pseudo-second-order equation kinetic model	925:970	The composite can adsorb both Co(II) and Ni(II) ions and the kinetic study confirmed a good agreement of experimental data with the pseudo-second-order equation kinetic model.
32840994	4	89	theme	metal	785:789	arg1	ions					791:794	metal ions	785:794	metal ions	785:794	The composite is synthesized at low temperature of 115 °C directly on the cellulose membrane (CM) in order to maintain the carboxylic and hydroxyl groups on the surface that are responsible for the adsorption of metal ions.
32840994	7	90	theme	rhodamine	1179:1187	arg1	B					1189:1189	rhodamine B	1179:1189	rhodamine B (RhB)	1179:1195	The bioinorganic composite demonstrates a synergistic effect in the photocatalytic degradation of rhodamine B (RhB) by exceeding the removal efficiency of single components.
32840994	7	90	theme	rhodamine	1179:1187	arg1	RhB					1192:1194	RhB	1192:1194	RhB	1192:1194	The bioinorganic composite demonstrates a synergistic effect in the photocatalytic degradation of rhodamine B (RhB) by exceeding the removal efficiency of single components.
32840994	8	91	theme	analytical	1332:1341	arg1	spectroscopy					1553:1564	X-ray photoelectron spectroscopy	1533:1564	X-ray photoelectron spectroscopy (XPS)	1533:1570	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	8	91	theme	analytical	1332:1341	arg1	techniques					1343:1352	various analytical techniques	1324:1352	various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS)	1324:1570	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	8	91	theme	analytical	1332:1341	arg1	diffraction					1510:1520	X-ray diffraction	1504:1520	X-ray diffraction (XRD)	1504:1526	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	8	91	theme	analytical	1332:1341	arg1	microscopy					1382:1391	scanning electron microscopy	1364:1391	scanning electron microscopy (SEM)	1364:1397	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	8	91	theme	analytical	1332:1341	arg1	microscopy					1431:1440	scanning transmission electron microscopy	1400:1440	scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping	1400:1501	The fabrication of the biologic-inorganic interface was confirmed by various analytical techniques including scanning electron microscopy (SEM), scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM EDX) mapping, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
32840994	3	92	theme	bioinorganic	450:461	arg1	membrane					470:477	The bioinorganic hybrid membrane	446:477	The bioinorganic hybrid membrane	446:477	The bioinorganic hybrid membrane consists of cellulose fibers to ensure mechanical stability and of Bi4O5Br2/BiOBr nanosheets.
32840994	5	93	theme	data	915:918	arg1	agreement					889:897	a good agreement	882:897	a good agreement of experimental data with the pseudo-second-order equation kinetic model	882:970	The composite can adsorb both Co(II) and Ni(II) ions and the kinetic study confirmed a good agreement of experimental data with the pseudo-second-order equation kinetic model.
32840994	9	94	theme	materials	1802:1810	arg1	functionality					1812:1824	materials functionality	1802:1824	materials functionality	1802:1824	The presented approach for controlled formation of the bioinorganic interface between natural material (cellulose) and nanoscopic inorganic materials of tailored morphology (Bi-O-Br system) enables the significant enhancement of materials functionality.
32840994	2	95	theme	ions	345:348	arg1	decomposition					410:422	photocatalytic decomposition	395:422	(ii) photocatalytic decomposition of organic compounds	390:443	Here, we demonstrate the fabrication of a hybrid material that can fulfill both tasks: (i) the adsorption of metal ions due to the negative surface charge, and (ii) photocatalytic decomposition of organic compounds.
32840994	2	95	theme	ions	345:348	arg1	tasks					310:314	both tasks	305:314	both tasks	305:314	Here, we demonstrate the fabrication of a hybrid material that can fulfill both tasks: (i) the adsorption of metal ions due to the negative surface charge, and (ii) photocatalytic decomposition of organic compounds.
32840994	2	95	theme	ions	345:348	arg1	adsorption					325:334	the adsorption	321:334	(i) the adsorption of metal ions due to the negative surface charge	317:383	Here, we demonstrate the fabrication of a hybrid material that can fulfill both tasks: (i) the adsorption of metal ions due to the negative surface charge, and (ii) photocatalytic decomposition of organic compounds.
34653482	3	0	theme	cyclic	779:784	arg1	CV					799:800	CV	799:800	CV	799:800	The electrochemical activity of egg white/ZnO rice structured composite SPCE was investigated for the oxidation-reduction of formaldehyde in alkaline media using cyclic voltammetry (CV).
34653482	3	0	theme	cyclic	779:784	arg1	voltammetry					786:796	cyclic voltammetry	779:796	cyclic voltammetry (CV)	779:801	The electrochemical activity of egg white/ZnO rice structured composite SPCE was investigated for the oxidation-reduction of formaldehyde in alkaline media using cyclic voltammetry (CV).
34653482	3	1	from	oxidation-reduction	719:737	arg1	media					767:771	alkaline media	758:771	alkaline media	758:771	The electrochemical activity of egg white/ZnO rice structured composite SPCE was investigated for the oxidation-reduction of formaldehyde in alkaline media using cyclic voltammetry (CV).
34653482	1	2	theme	white/ZnO	295:303	arg1	composite					321:329	egg white/ZnO rice structured composite	291:329	egg white/ZnO rice structured composite	291:329	In this study, Chitosan/ZnO nanocomposite (Ch/ZnO) and egg white/ZnO rice structured composite was synthesized by simple wet chemical technique and characterised by various techniques.
34653482	9	3	used	used	1657:1660	arg2	tool					1675:1678	practical tool	1665:1678	practical tool for formaldehyde analysis	1665:1704	The system described here can be easily be adapted to other substrates and used as practical tool for formaldehyde analysis.
34653482	9	3	used	used	1657:1660	arg2	system					1586:1591	The system	1582:1591	The system described here	1582:1606	The system described here can be easily be adapted to other substrates and used as practical tool for formaldehyde analysis.
34653482	0	4	with	sensor	127:132	arg1	nanocomposite					221:233	Chitosan/ZnO nanocomposite	208:233	Chitosan/ZnO nanocomposite	208:233	A disposable modified screen-printed electrode using egg white/ZnO rice structured composite as practical tool electrochemical sensor for formaldehyde detection and its comparative electrochemical study with Chitosan/ZnO nanocomposite.
34653482	3	5	theme	egg	649:651	arg1	SPCE					689:692	egg white/ZnO rice structured composite SPCE	649:692	egg white/ZnO rice structured composite SPCE	649:692	The electrochemical activity of egg white/ZnO rice structured composite SPCE was investigated for the oxidation-reduction of formaldehyde in alkaline media using cyclic voltammetry (CV).
34653482	5	6	theme	novel	972:976	arg1	sensor					993:998	a novel screen printed sensor	970:998	a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde	970:1086	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	5	6	theme	novel	972:976	arg1	composite					1039:1047	Egg white albumin/ZnO rice structured composite	1001:1047	Egg white albumin/ZnO rice structured composite	1001:1047	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	3	7	theme	composite	679:687	arg1	SPCE					689:692	egg white/ZnO rice structured composite SPCE	649:692	egg white/ZnO rice structured composite SPCE	649:692	The electrochemical activity of egg white/ZnO rice structured composite SPCE was investigated for the oxidation-reduction of formaldehyde in alkaline media using cyclic voltammetry (CV).
34653482	3	8	theme	rice	663:666	arg1	SPCE					689:692	egg white/ZnO rice structured composite SPCE	649:692	egg white/ZnO rice structured composite SPCE	649:692	The electrochemical activity of egg white/ZnO rice structured composite SPCE was investigated for the oxidation-reduction of formaldehyde in alkaline media using cyclic voltammetry (CV).
34653482	5	9	theme	white	1005:1009	arg1	sensor					993:998	a novel screen printed sensor	970:998	a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde	970:1086	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	5	9	theme	white	1005:1009	arg1	composite					1039:1047	Egg white albumin/ZnO rice structured composite	1001:1047	Egg white albumin/ZnO rice structured composite	1001:1047	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	4	10	theme	unique	809:814	arg1	activity					833:840	Their unique electrocatalytic activity	803:840	Their unique electrocatalytic activity for the formaldehyde	803:861	Their unique electrocatalytic activity for the formaldehyde found to exhibit 254 mV cathodic current response towards low negative potentials.
34653482	0	11	theme	electrochemical	181:195	arg1	study					197:201	its comparative electrochemical study	165:201	its comparative electrochemical study with Chitosan/ZnO nanocomposite	165:233	A disposable modified screen-printed electrode using egg white/ZnO rice structured composite as practical tool electrochemical sensor for formaldehyde detection and its comparative electrochemical study with Chitosan/ZnO nanocomposite.
34653482	5	12	theme	rice	1023:1026	arg1	sensor					993:998	a novel screen printed sensor	970:998	a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde	970:1086	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	5	12	theme	rice	1023:1026	arg1	composite					1039:1047	Egg white albumin/ZnO rice structured composite	1001:1047	Egg white albumin/ZnO rice structured composite	1001:1047	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	7	13	theme	formaldehyde	1321:1332	arg1	sensor					1334:1339	The developed electrochemical formaldehyde sensor	1291:1339	The developed electrochemical formaldehyde sensor	1291:1339	The developed electrochemical formaldehyde sensor was successfully applied as working electrode in cyclic voltammetric determination of formaldehyde in urine samples.
34653482	7	13	theme	formaldehyde	1321:1332	arg1	electrode					1377:1385	working electrode	1369:1385	working electrode in cyclic voltammetric determination of formaldehyde in urine samples	1369:1455	The developed electrochemical formaldehyde sensor was successfully applied as working electrode in cyclic voltammetric determination of formaldehyde in urine samples.
34653482	1	14	theme	chemical	361:368	arg1	technique					370:378	simple wet chemical technique	350:378	simple wet chemical technique	350:378	In this study, Chitosan/ZnO nanocomposite (Ch/ZnO) and egg white/ZnO rice structured composite was synthesized by simple wet chemical technique and characterised by various techniques.
34653482	5	15	theme	printed	985:991	arg1	sensor					993:998	a novel screen printed sensor	970:998	a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde	970:1086	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	5	15	theme	printed	985:991	arg1	composite					1039:1047	Egg white albumin/ZnO rice structured composite	1001:1047	Egg white albumin/ZnO rice structured composite	1001:1047	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	2	16	theme	printed	553:559	arg1	SPCE					572:575	egg white/ZnO rice structured composite modified screen printed electrode (SPCE)	497:576	egg white/ZnO rice structured composite modified screen printed electrode (SPCE)	497:576	A comparative electrochemical analysis were carried out and determined that egg white/ZnO rice structured composite modified screen printed electrode (SPCE) showed good electrochemical behaviour.
34653482	7	17	theme	developed	1295:1303	arg1	sensor					1334:1339	The developed electrochemical formaldehyde sensor	1291:1339	The developed electrochemical formaldehyde sensor	1291:1339	The developed electrochemical formaldehyde sensor was successfully applied as working electrode in cyclic voltammetric determination of formaldehyde in urine samples.
34653482	7	17	theme	developed	1295:1303	arg1	electrode					1377:1385	working electrode	1369:1385	working electrode in cyclic voltammetric determination of formaldehyde in urine samples	1369:1455	The developed electrochemical formaldehyde sensor was successfully applied as working electrode in cyclic voltammetric determination of formaldehyde in urine samples.
34653482	2	18	theme	good	585:588	arg1	behaviour					606:614	good electrochemical behaviour	585:614	good electrochemical behaviour	585:614	A comparative electrochemical analysis were carried out and determined that egg white/ZnO rice structured composite modified screen printed electrode (SPCE) showed good electrochemical behaviour.
34653482	0	19	theme	practical	96:104	arg1	sensor					127:132	practical tool electrochemical sensor	96:132	practical tool electrochemical sensor for formaldehyde detection	96:159	A disposable modified screen-printed electrode using egg white/ZnO rice structured composite as practical tool electrochemical sensor for formaldehyde detection and its comparative electrochemical study with Chitosan/ZnO nanocomposite.
34653482	9	20	theme	other	1636:1640	arg1	substrates					1642:1651	other substrates	1636:1651	other substrates	1636:1651	The system described here can be easily be adapted to other substrates and used as practical tool for formaldehyde analysis.
34653482	0	21	theme	electrochemical	111:125	arg1	sensor					127:132	practical tool electrochemical sensor	96:132	practical tool electrochemical sensor for formaldehyde detection	96:159	A disposable modified screen-printed electrode using egg white/ZnO rice structured composite as practical tool electrochemical sensor for formaldehyde detection and its comparative electrochemical study with Chitosan/ZnO nanocomposite.
34653482	5	22	theme	formaldehyde	1075:1086	arg1	determination					1058:1070	the determination	1054:1070	the determination of formaldehyde	1054:1086	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	2	23	theme	white/ZnO	501:509	arg1	SPCE					572:575	egg white/ZnO rice structured composite modified screen printed electrode (SPCE)	497:576	egg white/ZnO rice structured composite modified screen printed electrode (SPCE)	497:576	A comparative electrochemical analysis were carried out and determined that egg white/ZnO rice structured composite modified screen printed electrode (SPCE) showed good electrochemical behaviour.
34653482	0	24	theme	structured	72:81	arg1	composite					83:91	egg white/ZnO rice structured composite	53:91	egg white/ZnO rice structured composite	53:91	A disposable modified screen-printed electrode using egg white/ZnO rice structured composite as practical tool electrochemical sensor for formaldehyde detection and its comparative electrochemical study with Chitosan/ZnO nanocomposite.
34653482	2	25	theme	comparative	423:433	arg1	analysis					451:458	A comparative electrochemical analysis	421:458	A comparative electrochemical analysis	421:458	A comparative electrochemical analysis were carried out and determined that egg white/ZnO rice structured composite modified screen printed electrode (SPCE) showed good electrochemical behaviour.
34653482	1	26	theme	rice	305:308	arg1	composite					321:329	egg white/ZnO rice structured composite	291:329	egg white/ZnO rice structured composite	291:329	In this study, Chitosan/ZnO nanocomposite (Ch/ZnO) and egg white/ZnO rice structured composite was synthesized by simple wet chemical technique and characterised by various techniques.
34653482	2	27	theme	modified	537:544	arg1	SPCE					572:575	egg white/ZnO rice structured composite modified screen printed electrode (SPCE)	497:576	egg white/ZnO rice structured composite modified screen printed electrode (SPCE)	497:576	A comparative electrochemical analysis were carried out and determined that egg white/ZnO rice structured composite modified screen printed electrode (SPCE) showed good electrochemical behaviour.
34653482	3	28	theme	formaldehyde	742:753	arg1	oxidation-reduction					719:737	the oxidation-reduction	715:737	the oxidation-reduction of formaldehyde in alkaline media	715:771	The electrochemical activity of egg white/ZnO rice structured composite SPCE was investigated for the oxidation-reduction of formaldehyde in alkaline media using cyclic voltammetry (CV).
34653482	4	29	theme	low	921:923	arg1	potentials					934:943	low negative potentials	921:943	low negative potentials	921:943	Their unique electrocatalytic activity for the formaldehyde found to exhibit 254 mV cathodic current response towards low negative potentials.
34653482	9	30	theme	formaldehyde	1684:1695	arg1	analysis					1697:1704	formaldehyde analysis	1684:1704	formaldehyde analysis	1684:1704	The system described here can be easily be adapted to other substrates and used as practical tool for formaldehyde analysis.
34653482	7	31	theme	voltammetric	1397:1408	arg1	determination					1410:1422	cyclic voltammetric determination	1390:1422	cyclic voltammetric determination of formaldehyde in urine samples	1390:1455	The developed electrochemical formaldehyde sensor was successfully applied as working electrode in cyclic voltammetric determination of formaldehyde in urine samples.
34653482	2	32	theme	structured	516:525	arg1	SPCE					572:575	egg white/ZnO rice structured composite modified screen printed electrode (SPCE)	497:576	egg white/ZnO rice structured composite modified screen printed electrode (SPCE)	497:576	A comparative electrochemical analysis were carried out and determined that egg white/ZnO rice structured composite modified screen printed electrode (SPCE) showed good electrochemical behaviour.
34653482	0	33	theme	rice	67:70	arg1	composite					83:91	egg white/ZnO rice structured composite	53:91	egg white/ZnO rice structured composite	53:91	A disposable modified screen-printed electrode using egg white/ZnO rice structured composite as practical tool electrochemical sensor for formaldehyde detection and its comparative electrochemical study with Chitosan/ZnO nanocomposite.
34653482	0	34	theme	egg	53:55	arg1	composite					83:91	egg white/ZnO rice structured composite	53:91	egg white/ZnO rice structured composite	53:91	A disposable modified screen-printed electrode using egg white/ZnO rice structured composite as practical tool electrochemical sensor for formaldehyde detection and its comparative electrochemical study with Chitosan/ZnO nanocomposite.
34653482	1	35	theme	simple	350:355	arg1	technique					370:378	simple wet chemical technique	350:378	simple wet chemical technique	350:378	In this study, Chitosan/ZnO nanocomposite (Ch/ZnO) and egg white/ZnO rice structured composite was synthesized by simple wet chemical technique and characterised by various techniques.
34653482	7	36	from	determination	1410:1422	arg1	samples					1449:1455	urine samples	1443:1455	urine samples	1443:1455	The developed electrochemical formaldehyde sensor was successfully applied as working electrode in cyclic voltammetric determination of formaldehyde in urine samples.
34653482	6	37	theme	detection	1216:1224	arg1	6.2 nM					1232:1237	detection (LOD) 6.2 nM	1216:1237	detection (LOD) 6.2 nM	1216:1237	The sensor response was linear from 0.001 mM to 0.005 mM with limit of detection (LOD) 6.2 nM and their sensitivity was found to be 770.68 mM/μA.
34653482	4	38	theme	cathodic	887:894	arg1	response					904:911	254 mV cathodic current response	880:911	254 mV cathodic current response towards low negative potentials	880:943	Their unique electrocatalytic activity for the formaldehyde found to exhibit 254 mV cathodic current response towards low negative potentials.
34653482	1	39	theme	Chitosan/ZnO	251:262	arg1	Ch/ZnO					279:284	Ch/ZnO	279:284	Ch/ZnO	279:284	In this study, Chitosan/ZnO nanocomposite (Ch/ZnO) and egg white/ZnO rice structured composite was synthesized by simple wet chemical technique and characterised by various techniques.
34653482	1	39	theme	Chitosan/ZnO	251:262	arg1	nanocomposite					264:276	Chitosan/ZnO nanocomposite	251:276	Chitosan/ZnO nanocomposite (Ch/ZnO)	251:285	In this study, Chitosan/ZnO nanocomposite (Ch/ZnO) and egg white/ZnO rice structured composite was synthesized by simple wet chemical technique and characterised by various techniques.
34653482	7	40	theme	working	1369:1375	arg1	sensor					1334:1339	The developed electrochemical formaldehyde sensor	1291:1339	The developed electrochemical formaldehyde sensor	1291:1339	The developed electrochemical formaldehyde sensor was successfully applied as working electrode in cyclic voltammetric determination of formaldehyde in urine samples.
34653482	7	40	theme	working	1369:1375	arg1	electrode					1377:1385	working electrode	1369:1385	working electrode in cyclic voltammetric determination of formaldehyde in urine samples	1369:1455	The developed electrochemical formaldehyde sensor was successfully applied as working electrode in cyclic voltammetric determination of formaldehyde in urine samples.
34653482	3	41	theme	alkaline	758:765	arg1	media					767:771	alkaline media	758:771	alkaline media	758:771	The electrochemical activity of egg white/ZnO rice structured composite SPCE was investigated for the oxidation-reduction of formaldehyde in alkaline media using cyclic voltammetry (CV).
34653482	5	42	theme	differential	1107:1118	arg1	voltammetry					1126:1136	differential pulse voltammetry	1107:1136	differential pulse voltammetry (DPV)	1107:1142	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	5	42	theme	differential	1107:1118	arg1	DPV					1139:1141	DPV	1139:1141	DPV	1139:1141	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	0	43	theme	Chitosan/ZnO	208:219	arg1	nanocomposite					221:233	Chitosan/ZnO nanocomposite	208:233	Chitosan/ZnO nanocomposite	208:233	A disposable modified screen-printed electrode using egg white/ZnO rice structured composite as practical tool electrochemical sensor for formaldehyde detection and its comparative electrochemical study with Chitosan/ZnO nanocomposite.
34653482	6	44	from	0.005 mM	1193:1200	arg1	linear					1169:1174	linear	1169:1174	linear	1169:1174	The sensor response was linear from 0.001 mM to 0.005 mM with limit of detection (LOD) 6.2 nM and their sensitivity was found to be 770.68 mM/μA.
34653482	6	44	from	0.005 mM	1193:1200	arg1	response					1156:1163	The sensor response	1145:1163	The sensor response	1145:1163	The sensor response was linear from 0.001 mM to 0.005 mM with limit of detection (LOD) 6.2 nM and their sensitivity was found to be 770.68 mM/μA.
34653482	1	45	theme	egg	291:293	arg1	composite					321:329	egg white/ZnO rice structured composite	291:329	egg white/ZnO rice structured composite	291:329	In this study, Chitosan/ZnO nanocomposite (Ch/ZnO) and egg white/ZnO rice structured composite was synthesized by simple wet chemical technique and characterised by various techniques.
34653482	7	46	from	electrode	1377:1385	arg1	determination					1410:1422	cyclic voltammetric determination	1390:1422	cyclic voltammetric determination of formaldehyde in urine samples	1390:1455	The developed electrochemical formaldehyde sensor was successfully applied as working electrode in cyclic voltammetric determination of formaldehyde in urine samples.
34653482	6	47	theme	6.2 nM	1232:1237	arg1	limit					1207:1211	limit	1207:1211	limit of detection (LOD) 6.2 nM	1207:1237	The sensor response was linear from 0.001 mM to 0.005 mM with limit of detection (LOD) 6.2 nM and their sensitivity was found to be 770.68 mM/μA.
34653482	0	48	theme	formaldehyde	138:149	arg1	detection					151:159	formaldehyde detection	138:159	formaldehyde detection	138:159	A disposable modified screen-printed electrode using egg white/ZnO rice structured composite as practical tool electrochemical sensor for formaldehyde detection and its comparative electrochemical study with Chitosan/ZnO nanocomposite.
34653482	4	49	theme	negative	925:932	arg1	potentials					934:943	low negative potentials	921:943	low negative potentials	921:943	Their unique electrocatalytic activity for the formaldehyde found to exhibit 254 mV cathodic current response towards low negative potentials.
34653482	3	50	theme	electrochemical	621:635	arg1	activity					637:644	The electrochemical activity	617:644	The electrochemical activity of egg white/ZnO rice structured composite SPCE	617:692	The electrochemical activity of egg white/ZnO rice structured composite SPCE was investigated for the oxidation-reduction of formaldehyde in alkaline media using cyclic voltammetry (CV).
34653482	6	51	theme	LOD	1227:1229	arg1	6.2 nM					1232:1237	detection (LOD) 6.2 nM	1216:1237	detection (LOD) 6.2 nM	1216:1237	The sensor response was linear from 0.001 mM to 0.005 mM with limit of detection (LOD) 6.2 nM and their sensitivity was found to be 770.68 mM/μA.
34653482	3	52	theme	structured	668:677	arg1	SPCE					689:692	egg white/ZnO rice structured composite SPCE	649:692	egg white/ZnO rice structured composite SPCE	649:692	The electrochemical activity of egg white/ZnO rice structured composite SPCE was investigated for the oxidation-reduction of formaldehyde in alkaline media using cyclic voltammetry (CV).
34653482	8	53	dep	selective	1472:1480	arg1	sensor					1462:1467	The sensor	1458:1467	The sensor	1458:1467	The sensor is selective, inexpensive, stable over several days and disposable as well as simple to manufacture and operate.
34653482	8	53	dep	selective	1472:1480	arg1	disposable					1525:1534	disposable	1525:1534	disposable	1525:1534	The sensor is selective, inexpensive, stable over several days and disposable as well as simple to manufacture and operate.
34653482	8	53	dep	selective	1472:1480	arg1	stable					1496:1501	stable	1496:1501	stable	1496:1501	The sensor is selective, inexpensive, stable over several days and disposable as well as simple to manufacture and operate.
34653482	8	53	dep	selective	1472:1480	arg1	inexpensive					1483:1493	inexpensive	1483:1493	inexpensive	1483:1493	The sensor is selective, inexpensive, stable over several days and disposable as well as simple to manufacture and operate.
34653482	8	53	dep	selective	1472:1480	arg1	selective					1472:1480	selective	1472:1480	selective	1472:1480	The sensor is selective, inexpensive, stable over several days and disposable as well as simple to manufacture and operate.
34653482	2	54	theme	electrochemical	590:604	arg1	behaviour					606:614	good electrochemical behaviour	585:614	good electrochemical behaviour	585:614	A comparative electrochemical analysis were carried out and determined that egg white/ZnO rice structured composite modified screen printed electrode (SPCE) showed good electrochemical behaviour.
34653482	0	55	theme	comparative	169:179	arg1	study					197:201	its comparative electrochemical study	165:201	its comparative electrochemical study with Chitosan/ZnO nanocomposite	165:233	A disposable modified screen-printed electrode using egg white/ZnO rice structured composite as practical tool electrochemical sensor for formaldehyde detection and its comparative electrochemical study with Chitosan/ZnO nanocomposite.
34653482	3	56	theme	white/ZnO	653:661	arg1	SPCE					689:692	egg white/ZnO rice structured composite SPCE	649:692	egg white/ZnO rice structured composite SPCE	649:692	The electrochemical activity of egg white/ZnO rice structured composite SPCE was investigated for the oxidation-reduction of formaldehyde in alkaline media using cyclic voltammetry (CV).
34653482	5	57	theme	Egg	1001:1003	arg1	sensor					993:998	a novel screen printed sensor	970:998	a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde	970:1086	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	5	57	theme	Egg	1001:1003	arg1	composite					1039:1047	Egg white albumin/ZnO rice structured composite	1001:1047	Egg white albumin/ZnO rice structured composite	1001:1047	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	5	58	theme	albumin/ZnO	1011:1021	arg1	sensor					993:998	a novel screen printed sensor	970:998	a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde	970:1086	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	5	58	theme	albumin/ZnO	1011:1021	arg1	composite					1039:1047	Egg white albumin/ZnO rice structured composite	1001:1047	Egg white albumin/ZnO rice structured composite	1001:1047	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	7	59	theme	electrochemical	1305:1319	arg1	sensor					1334:1339	The developed electrochemical formaldehyde sensor	1291:1339	The developed electrochemical formaldehyde sensor	1291:1339	The developed electrochemical formaldehyde sensor was successfully applied as working electrode in cyclic voltammetric determination of formaldehyde in urine samples.
34653482	7	59	theme	electrochemical	1305:1319	arg1	electrode					1377:1385	working electrode	1369:1385	working electrode in cyclic voltammetric determination of formaldehyde in urine samples	1369:1455	The developed electrochemical formaldehyde sensor was successfully applied as working electrode in cyclic voltammetric determination of formaldehyde in urine samples.
34653482	3	60	theme	SPCE	689:692	arg1	activity					637:644	The electrochemical activity	617:644	The electrochemical activity of egg white/ZnO rice structured composite SPCE	617:692	The electrochemical activity of egg white/ZnO rice structured composite SPCE was investigated for the oxidation-reduction of formaldehyde in alkaline media using cyclic voltammetry (CV).
34653482	5	61	theme	screen	978:983	arg1	sensor					993:998	a novel screen printed sensor	970:998	a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde	970:1086	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	5	61	theme	screen	978:983	arg1	composite					1039:1047	Egg white albumin/ZnO rice structured composite	1001:1047	Egg white albumin/ZnO rice structured composite	1001:1047	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	2	62	theme	electrode	561:569	arg1	SPCE					572:575	egg white/ZnO rice structured composite modified screen printed electrode (SPCE)	497:576	egg white/ZnO rice structured composite modified screen printed electrode (SPCE)	497:576	A comparative electrochemical analysis were carried out and determined that egg white/ZnO rice structured composite modified screen printed electrode (SPCE) showed good electrochemical behaviour.
34653482	0	63	with	study	197:201	arg1	nanocomposite					221:233	Chitosan/ZnO nanocomposite	208:233	Chitosan/ZnO nanocomposite	208:233	A disposable modified screen-printed electrode using egg white/ZnO rice structured composite as practical tool electrochemical sensor for formaldehyde detection and its comparative electrochemical study with Chitosan/ZnO nanocomposite.
34653482	2	64	theme	screen	546:551	arg1	SPCE					572:575	egg white/ZnO rice structured composite modified screen printed electrode (SPCE)	497:576	egg white/ZnO rice structured composite modified screen printed electrode (SPCE)	497:576	A comparative electrochemical analysis were carried out and determined that egg white/ZnO rice structured composite modified screen printed electrode (SPCE) showed good electrochemical behaviour.
34653482	0	65	theme	tool	106:109	arg1	sensor					127:132	practical tool electrochemical sensor	96:132	practical tool electrochemical sensor for formaldehyde detection	96:159	A disposable modified screen-printed electrode using egg white/ZnO rice structured composite as practical tool electrochemical sensor for formaldehyde detection and its comparative electrochemical study with Chitosan/ZnO nanocomposite.
34653482	1	66	theme	various	401:407	arg1	techniques					409:418	various techniques	401:418	various techniques	401:418	In this study, Chitosan/ZnO nanocomposite (Ch/ZnO) and egg white/ZnO rice structured composite was synthesized by simple wet chemical technique and characterised by various techniques.
34653482	7	67	theme	urine	1443:1447	arg1	samples					1449:1455	urine samples	1443:1455	urine samples	1443:1455	The developed electrochemical formaldehyde sensor was successfully applied as working electrode in cyclic voltammetric determination of formaldehyde in urine samples.
34653482	2	68	theme	electrochemical	435:449	arg1	analysis					451:458	A comparative electrochemical analysis	421:458	A comparative electrochemical analysis	421:458	A comparative electrochemical analysis were carried out and determined that egg white/ZnO rice structured composite modified screen printed electrode (SPCE) showed good electrochemical behaviour.
34653482	5	69	theme	structured	1028:1037	arg1	sensor					993:998	a novel screen printed sensor	970:998	a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde	970:1086	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	5	69	theme	structured	1028:1037	arg1	composite					1039:1047	Egg white albumin/ZnO rice structured composite	1001:1047	Egg white albumin/ZnO rice structured composite	1001:1047	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	7	70	theme	formaldehyde	1427:1438	arg1	determination					1410:1422	cyclic voltammetric determination	1390:1422	cyclic voltammetric determination of formaldehyde in urine samples	1390:1455	The developed electrochemical formaldehyde sensor was successfully applied as working electrode in cyclic voltammetric determination of formaldehyde in urine samples.
34653482	2	71	theme	egg	497:499	arg1	SPCE					572:575	egg white/ZnO rice structured composite modified screen printed electrode (SPCE)	497:576	egg white/ZnO rice structured composite modified screen printed electrode (SPCE)	497:576	A comparative electrochemical analysis were carried out and determined that egg white/ZnO rice structured composite modified screen printed electrode (SPCE) showed good electrochemical behaviour.
34653482	4	72	theme	electrocatalytic	816:831	arg1	activity					833:840	Their unique electrocatalytic activity	803:840	Their unique electrocatalytic activity for the formaldehyde	803:861	Their unique electrocatalytic activity for the formaldehyde found to exhibit 254 mV cathodic current response towards low negative potentials.
34653482	0	73	theme	screen-printed	22:35	arg1	electrode					37:45	screen-printed electrode	22:45	screen-printed electrode	22:45	A disposable modified screen-printed electrode using egg white/ZnO rice structured composite as practical tool electrochemical sensor for formaldehyde detection and its comparative electrochemical study with Chitosan/ZnO nanocomposite.
34653482	1	74	theme	structured	310:319	arg1	composite					321:329	egg white/ZnO rice structured composite	291:329	egg white/ZnO rice structured composite	291:329	In this study, Chitosan/ZnO nanocomposite (Ch/ZnO) and egg white/ZnO rice structured composite was synthesized by simple wet chemical technique and characterised by various techniques.
34653482	9	75	theme	practical	1665:1673	arg1	system					1586:1591	The system	1582:1591	The system described here	1582:1606	The system described here can be easily be adapted to other substrates and used as practical tool for formaldehyde analysis.
34653482	9	75	theme	practical	1665:1673	arg1	tool					1675:1678	practical tool	1665:1678	practical tool for formaldehyde analysis	1665:1704	The system described here can be easily be adapted to other substrates and used as practical tool for formaldehyde analysis.
34653482	6	76	theme	sensor	1149:1154	arg1	linear					1169:1174	linear	1169:1174	linear	1169:1174	The sensor response was linear from 0.001 mM to 0.005 mM with limit of detection (LOD) 6.2 nM and their sensitivity was found to be 770.68 mM/μA.
34653482	6	76	theme	sensor	1149:1154	arg1	response					1156:1163	The sensor response	1145:1163	The sensor response	1145:1163	The sensor response was linear from 0.001 mM to 0.005 mM with limit of detection (LOD) 6.2 nM and their sensitivity was found to be 770.68 mM/μA.
34653482	5	77	theme	pulse	1120:1124	arg1	voltammetry					1126:1136	differential pulse voltammetry	1107:1136	differential pulse voltammetry (DPV)	1107:1142	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	5	77	theme	pulse	1120:1124	arg1	DPV					1139:1141	DPV	1139:1141	DPV	1139:1141	Based on these results, a novel screen printed sensor (Egg white albumin/ZnO rice structured composite) for the determination of formaldehyde was analysed using differential pulse voltammetry (DPV).
34653482	7	78	theme	cyclic	1390:1395	arg1	determination					1410:1422	cyclic voltammetric determination	1390:1422	cyclic voltammetric determination of formaldehyde in urine samples	1390:1455	The developed electrochemical formaldehyde sensor was successfully applied as working electrode in cyclic voltammetric determination of formaldehyde in urine samples.
34653482	2	79	theme	composite	527:535	arg1	SPCE					572:575	egg white/ZnO rice structured composite modified screen printed electrode (SPCE)	497:576	egg white/ZnO rice structured composite modified screen printed electrode (SPCE)	497:576	A comparative electrochemical analysis were carried out and determined that egg white/ZnO rice structured composite modified screen printed electrode (SPCE) showed good electrochemical behaviour.
34653482	0	80	theme	white/ZnO	57:65	arg1	composite					83:91	egg white/ZnO rice structured composite	53:91	egg white/ZnO rice structured composite	53:91	A disposable modified screen-printed electrode using egg white/ZnO rice structured composite as practical tool electrochemical sensor for formaldehyde detection and its comparative electrochemical study with Chitosan/ZnO nanocomposite.
34653482	6	81	with	linear	1169:1174	arg1	limit					1207:1211	limit	1207:1211	limit of detection (LOD) 6.2 nM	1207:1237	The sensor response was linear from 0.001 mM to 0.005 mM with limit of detection (LOD) 6.2 nM and their sensitivity was found to be 770.68 mM/μA.
34653482	2	82	theme	rice	511:514	arg1	SPCE					572:575	egg white/ZnO rice structured composite modified screen printed electrode (SPCE)	497:576	egg white/ZnO rice structured composite modified screen printed electrode (SPCE)	497:576	A comparative electrochemical analysis were carried out and determined that egg white/ZnO rice structured composite modified screen printed electrode (SPCE) showed good electrochemical behaviour.
34653482	6	83	dep	0.005 mM	1193:1200	arg1	to					1190:1191	to	1190:1191	to	1190:1191	The sensor response was linear from 0.001 mM to 0.005 mM with limit of detection (LOD) 6.2 nM and their sensitivity was found to be 770.68 mM/μA.
34653482	1	84	theme	wet	357:359	arg1	technique					370:378	simple wet chemical technique	350:378	simple wet chemical technique	350:378	In this study, Chitosan/ZnO nanocomposite (Ch/ZnO) and egg white/ZnO rice structured composite was synthesized by simple wet chemical technique and characterised by various techniques.
34653482	4	85	theme	254 mV	880:885	arg1	response					904:911	254 mV cathodic current response	880:911	254 mV cathodic current response towards low negative potentials	880:943	Their unique electrocatalytic activity for the formaldehyde found to exhibit 254 mV cathodic current response towards low negative potentials.
34653482	8	86	theme	several	1508:1514	arg1	days					1516:1519	several days	1508:1519	several days	1508:1519	The sensor is selective, inexpensive, stable over several days and disposable as well as simple to manufacture and operate.
34653482	4	87	theme	current	896:902	arg1	response					904:911	254 mV cathodic current response	880:911	254 mV cathodic current response towards low negative potentials	880:943	Their unique electrocatalytic activity for the formaldehyde found to exhibit 254 mV cathodic current response towards low negative potentials.
34677020	20	0	theme	starch	3060:3065	arg1	temperature					3079:3089	the starch dissolution temperature	3056:3089	the starch dissolution temperature	3056:3089	The effect of the starch dissolution temperature on the AF4 characterization of starch was also investigated.
34677020	4	1	theme	samples	795:801	arg1	distribution					775:786	the size distribution	766:786	the size distribution of the samples	766:801	The major difference between AF4 and dynamic light scattering or microscopy techniques is that AF4 enables the separation of particles based on their size; consequently, the elution profile can be converted to the size distribution of the samples.
34677020	25	2	theme	size	3878:3881	arg1	characterization					3883:3898	the size characterization	3874:3898	the size characterization of starch granules	3874:3917	The results demonstrated that the AF4 system developed in this study is rapid and accurate for the size characterization of starch granules.
34677020	22	3	theme	radius	3422:3427	arg1	ratio					3409:3413	the ratio	3405:3413	the ratio of the radius of gyration (Rg) to the hydrodynamic radius (Rh) of rice starch and sweet potato starch	3405:3515	The results showed that the ratio of the radius of gyration (Rg) to the hydrodynamic radius (Rh) of rice starch and sweet potato starch is in the range of 0.9-1.1 over the molar mass range of 10 6-108 g/mol.
34677020	10	4	theme	polystyrene	1690:1700	arg1	mixture					1679:1685	a mixture	1677:1685	a mixture of polystyrene (PS)	1677:1705	The performance of the developed AF4 system was evaluated by running a mixture of polystyrene (PS) with diameter of 2, 6, 12, and 20 μm, respectively.
34677020	17	5	theme	0.01	2549:2552	arg1	%					2553:2553	%	2553:2553	%	2553:2553	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	11	6	theme	μm	1837:1838	arg1	PS					1840:1841	6 μm PS	1835:1841	6 μm PS	1835:1841	Baseline separation of four PS samples was achieved, and the resolution for 6 μm PS and 12 μm PS was 1.40.
34677020	14	7	theme	granules	2150:2157	arg1	separation					2129:2138	the AF4 separation	2121:2138	the AF4 separation of starch granules	2121:2157	The effect of the composition of the carrier liquid on the AF4 separation of starch granules was also studied.
34677020	6	8	theme	size	1074:1077	arg1	SEC					1105:1107	SEC	1105:1107	SEC	1105:1107	Unlike size exclusion chromatography (SEC), AF4 systems use an open channel that does not require a stationary phase or packing materials.
34677020	6	8	theme	size	1074:1077	arg1	chromatography					1089:1102	size exclusion chromatography	1074:1102	size exclusion chromatography (SEC)	1074:1108	Unlike size exclusion chromatography (SEC), AF4 systems use an open channel that does not require a stationary phase or packing materials.
34677020	12	9	theme	AF4	1903:1905	arg1	system					1907:1912	the developed AF4 system	1889:1912	the developed AF4 system	1889:1912	The detection limit of the developed AF4 system was higher than that of commercial AF4 systems.
34677020	19	10	theme	molecular	3004:3012	arg1	characterization					3014:3029	the reliable molecular characterization	2991:3029	the reliable molecular characterization of starch	2991:3039	A molecularly dispersed solution is necessary for the reliable molecular characterization of starch.
34677020	3	11	theme	field-flow	487:496	arg1	AF4					513:515	AF4	513:515	AF4	513:515	Asymmetrical flow field-flow fractionation (AF4) is a size-based separation technique.
34677020	3	11	theme	field-flow	487:496	arg1	technique					545:553	a size-based separation technique	521:553	a size-based separation technique	521:553	Asymmetrical flow field-flow fractionation (AF4) is a size-based separation technique.
34677020	3	11	theme	field-flow	487:496	arg1	fractionation					498:510	Asymmetrical flow field-flow fractionation	469:510	Asymmetrical flow field-flow fractionation (AF4)	469:516	Asymmetrical flow field-flow fractionation (AF4) is a size-based separation technique.
34677020	4	12	theme	size	770:773	arg1	distribution					775:786	the size distribution	766:786	the size distribution of the samples	766:801	The major difference between AF4 and dynamic light scattering or microscopy techniques is that AF4 enables the separation of particles based on their size; consequently, the elution profile can be converted to the size distribution of the samples.
34677020	24	13	theme	rice	3733:3736	arg1	molecules					3745:3753	rice starch molecules	3733:3753	rice starch molecules	3733:3753	Rice starch has the highest apparent density among the three starches, indicating that rice starch molecules have a dense structure.
34677020	4	14	theme	light	601:605	arg1	scattering					607:616	dynamic light scattering	593:616	dynamic light scattering	593:616	The major difference between AF4 and dynamic light scattering or microscopy techniques is that AF4 enables the separation of particles based on their size; consequently, the elution profile can be converted to the size distribution of the samples.
34677020	6	15	theme	open	1130:1133	arg1	channel					1135:1141	an open channel	1127:1141	an open channel that does not require a stationary phase or packing materials	1127:1203	Unlike size exclusion chromatography (SEC), AF4 systems use an open channel that does not require a stationary phase or packing materials.
34677020	3	16	theme	Asymmetrical	469:480	arg1	AF4					513:515	AF4	513:515	AF4	513:515	Asymmetrical flow field-flow fractionation (AF4) is a size-based separation technique.
34677020	3	16	theme	Asymmetrical	469:480	arg1	technique					545:553	a size-based separation technique	521:553	a size-based separation technique	521:553	Asymmetrical flow field-flow fractionation (AF4) is a size-based separation technique.
34677020	3	16	theme	Asymmetrical	469:480	arg1	fractionation					498:510	Asymmetrical flow field-flow fractionation	469:510	Asymmetrical flow field-flow fractionation (AF4)	469:516	Asymmetrical flow field-flow fractionation (AF4) is a size-based separation technique.
34677020	1	17	theme	granules	175:182	arg1	form					151:154	the form	147:154	the form of semicrystalline granules	147:182	Starch occurs naturally in the form of semicrystalline granules, and is composed of two types of carbohydrate molecules, amylose (AM) and amylopectin (AP).
34677020	14	18	theme	AF4	2125:2127	arg1	separation					2129:2138	the AF4 separation	2121:2138	the AF4 separation of starch granules	2121:2157	The effect of the composition of the carrier liquid on the AF4 separation of starch granules was also studied.
34677020	24	19	contain	have	3755:3758	arg1	molecules					3745:3753	rice starch molecules	3733:3753	rice starch molecules	3733:3753	Rice starch has the highest apparent density among the three starches, indicating that rice starch molecules have a dense structure.
34677020	24	19	contain	have	3755:3758	arg2	structure					3768:3776	a dense structure	3760:3776	a dense structure	3760:3776	Rice starch has the highest apparent density among the three starches, indicating that rice starch molecules have a dense structure.
34677020	20	20	theme	starch	3122:3127	arg1	characterization					3102:3117	the AF4 characterization	3094:3117	the AF4 characterization of starch	3094:3127	The effect of the starch dissolution temperature on the AF4 characterization of starch was also investigated.
34677020	20	21	from	effect	3046:3051	arg1	characterization					3102:3117	the AF4 characterization	3094:3117	the AF4 characterization of starch	3094:3127	The effect of the starch dissolution temperature on the AF4 characterization of starch was also investigated.
34677020	7	22	theme	AF4	1254:1256	arg1	separation					1258:1267	AF4 separation	1254:1267	AF4 separation	1254:1267	Thus, the shear scission of AP molecules during AF4 separation is minimized.
34677020	22	23	theme	hydrodynamic	3453:3464	arg1	radius					3466:3471	the hydrodynamic radius	3449:3471	the hydrodynamic radius (Rh) of rice starch and sweet potato starch	3449:3515	The results showed that the ratio of the radius of gyration (Rg) to the hydrodynamic radius (Rh) of rice starch and sweet potato starch is in the range of 0.9-1.1 over the molar mass range of 10 6-108 g/mol.
34677020	22	23	theme	hydrodynamic	3453:3464	arg1	Rh					3474:3475	Rh	3474:3475	Rh	3474:3475	The results showed that the ratio of the radius of gyration (Rg) to the hydrodynamic radius (Rh) of rice starch and sweet potato starch is in the range of 0.9-1.1 over the molar mass range of 10 6-108 g/mol.
34677020	20	24	theme	temperature	3079:3089	arg1	effect					3046:3051	The effect	3042:3051	The effect of the starch dissolution temperature on the AF4 characterization of starch	3042:3127	The effect of the starch dissolution temperature on the AF4 characterization of starch was also investigated.
34677020	23	25	theme	Rg/Rh	3610:3614	arg1	ratio					3616:3620	the Rg/Rh ratio	3606:3620	the Rg/Rh ratio	3606:3620	For rice starch, the Rg/Rh ratio is between 1.2 and 1.4.
34677020	11	26	theme	PS	1787:1788	arg1	samples					1790:1796	four PS samples	1782:1796	four PS samples	1782:1796	Baseline separation of four PS samples was achieved, and the resolution for 6 μm PS and 12 μm PS was 1.40.
34677020	7	27	theme	molecules	1237:1245	arg1	scission					1222:1229	the shear scission	1212:1229	the shear scission of AP molecules during AF4 separation	1212:1267	Thus, the shear scission of AP molecules during AF4 separation is minimized.
34677020	6	28	theme	packing	1187:1193	arg1	materials					1195:1203	packing materials	1187:1203	packing materials	1187:1203	Unlike size exclusion chromatography (SEC), AF4 systems use an open channel that does not require a stationary phase or packing materials.
34677020	21	29	theme	botanical	3346:3354	arg1	origins					3356:3362	the different botanical origins	3332:3362	the different botanical origins of the granules	3332:3378	The optimal dissolution temperature for lotus seed and rice starch granules was 75 ℃, while that for sweet potato starch granules was 78 ℃; this difference is mainly attributed to the different botanical origins of the granules.
34677020	3	30	theme	separation	534:543	arg1	fractionation					498:510	Asymmetrical flow field-flow fractionation	469:510	Asymmetrical flow field-flow fractionation (AF4)	469:516	Asymmetrical flow field-flow fractionation (AF4) is a size-based separation technique.
34677020	3	30	theme	separation	534:543	arg1	technique					545:553	a size-based separation technique	521:553	a size-based separation technique	521:553	Asymmetrical flow field-flow fractionation (AF4) is a size-based separation technique.
34677020	9	31	theme	starch	1524:1529	arg1	granules					1531:1538	starch granules	1524:1538	starch granules extracted from sweet potato, lotus seed, and rice	1524:1588	In this study, a home-made AF4 system was developed, and its capability for the size characterization of starch granules extracted from sweet potato, lotus seed, and rice was investigated.
34677020	16	32	theme	size	2414:2417	arg1	characterization					2419:2434	size characterization	2414:2434	size characterization of the starch granules	2414:2457	The results revealed that the type of dispersant and viscosity of the carrier liquid affect the accuracy of size characterization of the starch granules.
34677020	17	33	with	agreement	2756:2764	arg1	that					2771:2774	that	2771:2774	that	2771:2774	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	24	34	theme	apparent	3674:3681	arg1	density					3683:3689	the highest apparent density	3662:3689	the highest apparent density among the three starches	3662:3714	Rice starch has the highest apparent density among the three starches, indicating that rice starch molecules have a dense structure.
34677020	26	35	theme	technical	4044:4052	arg1	support					4054:4060	technical support	4044:4060	technical support	4044:4060	The developed AF4 system, when combined with commercial AF4 systems coupled online with MALS and dRI detectors, can provide technical support to study the relationship between the size from the nanoscale to the microscale and functional properties of starch.
34677020	5	36	theme	AF4	829:831	arg1	systems					833:839	AF4 systems	829:839	AF4 systems	829:839	In the last two decades, AF4 systems, when coupled online with multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI), have demonstrated to be applicable for the size characterization of starch at the molecular level.
34677020	23	37	theme	rice	3593:3596	arg1	starch					3598:3603	rice starch	3593:3603	rice starch	3593:3603	For rice starch, the Rg/Rh ratio is between 1.2 and 1.4.
34677020	8	38	theme	AF4	1324:1326	arg1	system					1328:1333	a commercial AF4 system	1311:1333	a commercial AF4 system	1311:1333	The size detection range of a commercial AF4 system ranges from 1 nm to 10 μm, which is smaller than the size range of starch granules.
34677020	7	39	theme	shear	1216:1220	arg1	scission					1222:1229	the shear scission	1212:1229	the shear scission of AP molecules during AF4 separation	1212:1267	Thus, the shear scission of AP molecules during AF4 separation is minimized.
34677020	21	40	theme	optimal	3156:3162	arg1	℃					3235:3235	75 ℃	3232:3235	75 ℃	3232:3235	The optimal dissolution temperature for lotus seed and rice starch granules was 75 ℃, while that for sweet potato starch granules was 78 ℃; this difference is mainly attributed to the different botanical origins of the granules.
34677020	21	40	theme	optimal	3156:3162	arg1	temperature					3176:3186	The optimal dissolution temperature	3152:3186	The optimal dissolution temperature for lotus seed and rice starch granules	3152:3226	The optimal dissolution temperature for lotus seed and rice starch granules was 75 ℃, while that for sweet potato starch granules was 78 ℃; this difference is mainly attributed to the different botanical origins of the granules.
34677020	25	41	theme	granules	3910:3917	arg1	characterization					3883:3898	the size characterization	3874:3898	the size characterization of starch granules	3874:3917	The results demonstrated that the AF4 system developed in this study is rapid and accurate for the size characterization of starch granules.
34677020	13	42	theme	developed	1972:1980	arg1	promising					1996:2004	promising	1996:2004	promising	1996:2004	Thus, the developed AF4 system is promising for the separation and characterization of starch granules.
34677020	13	42	theme	developed	1972:1980	arg1	system					1986:1991	the developed AF4 system	1968:1991	the developed AF4 system	1968:1991	Thus, the developed AF4 system is promising for the separation and characterization of starch granules.
34677020	9	43	theme	sweet	1555:1559	arg1	potato					1561:1566	sweet potato	1555:1566	sweet potato	1555:1566	In this study, a home-made AF4 system was developed, and its capability for the size characterization of starch granules extracted from sweet potato, lotus seed, and rice was investigated.
34677020	16	44	theme	starch	2443:2448	arg1	granules					2450:2457	the starch granules	2439:2457	the starch granules	2439:2457	The results revealed that the type of dispersant and viscosity of the carrier liquid affect the accuracy of size characterization of the starch granules.
34677020	15	45	from	accuracy	2191:2198	arg1	terms					2210:2214	terms	2210:2214	terms of size characterization of the starch granules	2210:2262	Moreover, the accuracy of AF4 in terms of size characterization of the starch granules was evaluated by optical microscopy (OM).
34677020	26	46	from	nanoscale	4114:4122	arg1	size					4100:4103	the size	4096:4103	the size from the nanoscale to the microscale and functional properties of starch	4096:4176	The developed AF4 system, when combined with commercial AF4 systems coupled online with MALS and dRI detectors, can provide technical support to study the relationship between the size from the nanoscale to the microscale and functional properties of starch.
34677020	17	47	theme	carrier	2733:2739	arg1	viscosity					2716:2724	the viscosity	2712:2724	the viscosity of the carrier liquid	2712:2746	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	26	48	theme	starch	4171:4176	arg1	properties					4157:4166	the microscale and functional properties	4127:4166	the microscale and functional properties of starch	4127:4176	The developed AF4 system, when combined with commercial AF4 systems coupled online with MALS and dRI detectors, can provide technical support to study the relationship between the size from the nanoscale to the microscale and functional properties of starch.
34677020	17	49	theme	%	2612:2612	arg1	NaN3					2620:2623	0.02% (w/v) NaN3	2608:2623	0.02% (w/v) NaN3 (used as a bactericide)	2608:2647	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	5	50	theme	last	811:814	arg1	decades					820:826	the last two decades	807:826	the last two decades	807:826	In the last two decades, AF4 systems, when coupled online with multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI), have demonstrated to be applicable for the size characterization of starch at the molecular level.
34677020	22	51	theme	starch	3510:3515	arg1	radius					3466:3471	the hydrodynamic radius	3449:3471	the hydrodynamic radius (Rh) of rice starch and sweet potato starch	3449:3515	The results showed that the ratio of the radius of gyration (Rg) to the hydrodynamic radius (Rh) of rice starch and sweet potato starch is in the range of 0.9-1.1 over the molar mass range of 10 6-108 g/mol.
34677020	22	51	theme	starch	3510:3515	arg1	Rh					3474:3475	Rh	3474:3475	Rh	3474:3475	The results showed that the ratio of the radius of gyration (Rg) to the hydrodynamic radius (Rh) of rice starch and sweet potato starch is in the range of 0.9-1.1 over the molar mass range of 10 6-108 g/mol.
34677020	0	52	theme	size	77:80	arg1	characterization					82:97	the size characterization	73:97	the size characterization of starch granules	73:116	[Development of an asymmetrical flow field-flow fractionation system for the size characterization of starch granules].
34677020	15	53	theme	AF4	2203:2205	arg1	accuracy					2191:2198	the accuracy	2187:2198	the accuracy of AF4 in terms of size characterization of the starch granules	2187:2262	Moreover, the accuracy of AF4 in terms of size characterization of the starch granules was evaluated by optical microscopy (OM).
34677020	18	54	theme	dRI	2853:2855	arg1	detectors					2857:2865	dRI detectors	2853:2865	dRI detectors	2853:2865	Furthermore, a commercial AF4 system coupled with MALS and dRI detectors was employed for the separation and characterization of starch molecules.
34677020	2	55	theme	starch	461:466	arg1	properties					447:456	the functional properties	432:456	the functional properties of starch	432:466	Starch granules and starch molecules have sizes in the range of 1-100 μm and 20-250 nm, respectively; these size ranges are among the key factors affecting the functional properties of starch.
34677020	22	56	theme	sweet	3497:3501	arg1	starch					3510:3515	sweet potato starch	3497:3515	sweet potato starch	3497:3515	The results showed that the ratio of the radius of gyration (Rg) to the hydrodynamic radius (Rh) of rice starch and sweet potato starch is in the range of 0.9-1.1 over the molar mass range of 10 6-108 g/mol.
34677020	2	57	contain	have	313:316	arg1	granules					283:290	Starch granules	276:290	Starch granules	276:290	Starch granules and starch molecules have sizes in the range of 1-100 μm and 20-250 nm, respectively; these size ranges are among the key factors affecting the functional properties of starch.
34677020	2	57	contain	have	313:316	arg1	molecules					303:311	starch molecules	296:311	starch molecules	296:311	Starch granules and starch molecules have sizes in the range of 1-100 μm and 20-250 nm, respectively; these size ranges are among the key factors affecting the functional properties of starch.
34677020	2	57	contain	have	313:316	arg2	sizes					318:322	sizes	318:322	sizes	318:322	Starch granules and starch molecules have sizes in the range of 1-100 μm and 20-250 nm, respectively; these size ranges are among the key factors affecting the functional properties of starch.
34677020	22	58	theme	g/mol	3582:3586	arg1	range					3564:3568	the molar mass range	3549:3568	the molar mass range of 10 6-108 g/mol	3549:3586	The results showed that the ratio of the radius of gyration (Rg) to the hydrodynamic radius (Rh) of rice starch and sweet potato starch is in the range of 0.9-1.1 over the molar mass range of 10 6-108 g/mol.
34677020	18	59	theme	commercial	2809:2818	arg1	system					2824:2829	a commercial AF4 system	2807:2829	a commercial AF4 system coupled with MALS and dRI detectors	2807:2865	Furthermore, a commercial AF4 system coupled with MALS and dRI detectors was employed for the separation and characterization of starch molecules.
34677020	5	60	theme	light	878:882	arg1	MALS					896:899	MALS	896:899	MALS	896:899	In the last two decades, AF4 systems, when coupled online with multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI), have demonstrated to be applicable for the size characterization of starch at the molecular level.
34677020	5	60	theme	light	878:882	arg1	scattering					884:893	multiangle light scattering	867:893	multiangle light scattering (MALS)	867:900	In the last two decades, AF4 systems, when coupled online with multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI), have demonstrated to be applicable for the size characterization of starch at the molecular level.
34677020	17	61	theme	rice	2485:2488	arg1	granules					2497:2504	rice starch granules	2485:2504	rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose	2485:2694	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	12	62	theme	commercial	1938:1947	arg1	systems					1953:1959	commercial AF4 systems	1938:1959	commercial AF4 systems	1938:1959	The detection limit of the developed AF4 system was higher than that of commercial AF4 systems.
34677020	22	63	theme	starch	3486:3491	arg1	radius					3466:3471	the hydrodynamic radius	3449:3471	the hydrodynamic radius (Rh) of rice starch and sweet potato starch	3449:3515	The results showed that the ratio of the radius of gyration (Rg) to the hydrodynamic radius (Rh) of rice starch and sweet potato starch is in the range of 0.9-1.1 over the molar mass range of 10 6-108 g/mol.
34677020	22	63	theme	starch	3486:3491	arg1	Rh					3474:3475	Rh	3474:3475	Rh	3474:3475	The results showed that the ratio of the radius of gyration (Rg) to the hydrodynamic radius (Rh) of rice starch and sweet potato starch is in the range of 0.9-1.1 over the molar mass range of 10 6-108 g/mol.
34677020	17	64	theme	dodecyl	2568:2574	arg1	sulfate					2576:2582	0.01% (w/v) sodium dodecyl sulfate	2549:2582	0.01% (w/v) sodium dodecyl sulfate (used as a dispersant)	2549:2605	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	21	65	theme	starch	3212:3217	arg1	granules					3219:3226	rice starch granules	3207:3226	rice starch granules	3207:3226	The optimal dissolution temperature for lotus seed and rice starch granules was 75 ℃, while that for sweet potato starch granules was 78 ℃; this difference is mainly attributed to the different botanical origins of the granules.
34677020	2	66	theme	μm	346:347	arg1	range					331:335	the range	327:335	the range of 1-100 μm and 20-250 nm	327:361	Starch granules and starch molecules have sizes in the range of 1-100 μm and 20-250 nm, respectively; these size ranges are among the key factors affecting the functional properties of starch.
34677020	0	67	theme	granules	109:116	arg1	characterization					82:97	the size characterization	73:97	the size characterization of starch granules	73:116	[Development of an asymmetrical flow field-flow fractionation system for the size characterization of starch granules].
34677020	21	68	theme	granules	3371:3378	arg1	origins					3356:3362	the different botanical origins	3332:3362	the different botanical origins of the granules	3332:3378	The optimal dissolution temperature for lotus seed and rice starch granules was 75 ℃, while that for sweet potato starch granules was 78 ℃; this difference is mainly attributed to the different botanical origins of the granules.
34677020	16	69	theme	dispersant	2344:2353	arg1	type					2336:2339	the type	2332:2339	the type of dispersant and viscosity of the carrier liquid	2332:2389	The results revealed that the type of dispersant and viscosity of the carrier liquid affect the accuracy of size characterization of the starch granules.
34677020	2	70	theme	size	384:387	arg1	ranges					389:394	these size ranges	378:394	these size ranges	378:394	Starch granules and starch molecules have sizes in the range of 1-100 μm and 20-250 nm, respectively; these size ranges are among the key factors affecting the functional properties of starch.
34677020	4	71	theme	particles	681:689	arg1	separation					667:676	the separation	663:676	the separation of particles based on their size	663:709	The major difference between AF4 and dynamic light scattering or microscopy techniques is that AF4 enables the separation of particles based on their size; consequently, the elution profile can be converted to the size distribution of the samples.
34677020	26	72	theme	developed	3924:3932	arg1	system					3938:3943	The developed AF4 system	3920:3943	The developed AF4 system	3920:3943	The developed AF4 system, when combined with commercial AF4 systems coupled online with MALS and dRI detectors, can provide technical support to study the relationship between the size from the nanoscale to the microscale and functional properties of starch.
34677020	11	73	theme	μm	1850:1851	arg1	PS					1853:1854	12 μm PS	1847:1854	12 μm PS	1847:1854	Baseline separation of four PS samples was achieved, and the resolution for 6 μm PS and 12 μm PS was 1.40.
34677020	16	74	theme	viscosity	2359:2367	arg1	type					2336:2339	the type	2332:2339	the type of dispersant and viscosity of the carrier liquid	2332:2389	The results revealed that the type of dispersant and viscosity of the carrier liquid affect the accuracy of size characterization of the starch granules.
34677020	21	75	theme	lotus	3192:3196	arg1	seed					3198:3201	lotus seed	3192:3201	lotus seed	3192:3201	The optimal dissolution temperature for lotus seed and rice starch granules was 75 ℃, while that for sweet potato starch granules was 78 ℃; this difference is mainly attributed to the different botanical origins of the granules.
34677020	17	76	theme	sodium	2561:2566	arg1	sulfate					2576:2582	0.01% (w/v) sodium dodecyl sulfate	2549:2582	0.01% (w/v) sodium dodecyl sulfate (used as a dispersant)	2549:2605	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	18	77	dep	separation	2888:2897	arg1	the					2884:2886	the	2884:2886	the	2884:2886	Furthermore, a commercial AF4 system coupled with MALS and dRI detectors was employed for the separation and characterization of starch molecules.
34677020	21	78	theme	sweet	3253:3257	arg1	granules					3273:3280	sweet potato starch granules	3253:3280	sweet potato starch granules	3253:3280	The optimal dissolution temperature for lotus seed and rice starch granules was 75 ℃, while that for sweet potato starch granules was 78 ℃; this difference is mainly attributed to the different botanical origins of the granules.
34677020	22	79	theme	0.9-1.1	3536:3542	arg1	range					3527:3531	the range	3523:3531	the range of 0.9-1.1	3523:3542	The results showed that the ratio of the radius of gyration (Rg) to the hydrodynamic radius (Rh) of rice starch and sweet potato starch is in the range of 0.9-1.1 over the molar mass range of 10 6-108 g/mol.
34677020	13	80	dep	separation	2014:2023	arg1	the					2010:2012	the	2010:2012	the	2010:2012	Thus, the developed AF4 system is promising for the separation and characterization of starch granules.
34677020	0	81	theme	flow	32:35	arg1	system					62:67	an asymmetrical flow field-flow fractionation system	16:67	an asymmetrical flow field-flow fractionation system for the size characterization of starch granules	16:116	[Development of an asymmetrical flow field-flow fractionation system for the size characterization of starch granules].
34677020	5	82	theme	starch	1036:1041	arg1	characterization					1016:1031	the size characterization	1007:1031	the size characterization of starch at the molecular level	1007:1064	In the last two decades, AF4 systems, when coupled online with multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI), have demonstrated to be applicable for the size characterization of starch at the molecular level.
34677020	5	83	theme	index	930:934	arg1	AF4-MALS-dRI					953:964	AF4-MALS-dRI	953:964	AF4-MALS-dRI	953:964	In the last two decades, AF4 systems, when coupled online with multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI), have demonstrated to be applicable for the size characterization of starch at the molecular level.
34677020	5	83	theme	index	930:934	arg1	detectors					942:950	multiangle light scattering (MALS) and differential refractive index (dRI) detectors	867:950	multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI)	867:965	In the last two decades, AF4 systems, when coupled online with multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI), have demonstrated to be applicable for the size characterization of starch at the molecular level.
34677020	16	84	theme	liquid	2384:2389	arg1	carrier					2376:2382	the carrier liquid	2372:2389	the carrier liquid	2372:2389	The results revealed that the type of dispersant and viscosity of the carrier liquid affect the accuracy of size characterization of the starch granules.
34677020	10	85	theme	AF4	1641:1643	arg1	system					1645:1650	the developed AF4 system	1627:1650	the developed AF4 system	1627:1650	The performance of the developed AF4 system was evaluated by running a mixture of polystyrene (PS) with diameter of 2, 6, 12, and 20 μm, respectively.
34677020	19	86	theme	reliable	2995:3002	arg1	characterization					3014:3029	the reliable molecular characterization	2991:3029	the reliable molecular characterization of starch	2991:3039	A molecularly dispersed solution is necessary for the reliable molecular characterization of starch.
34677020	17	87	theme	granules	2497:2504	arg1	distribution					2469:2480	The size distribution	2460:2480	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid)	2460:2747	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	0	88	theme	fractionation	48:60	arg1	system					62:67	an asymmetrical flow field-flow fractionation system	16:67	an asymmetrical flow field-flow fractionation system for the size characterization of starch granules	16:116	[Development of an asymmetrical flow field-flow fractionation system for the size characterization of starch granules].
34677020	24	89	theme	Rice	3646:3649	arg1	starch					3651:3656	Rice starch	3646:3656	Rice starch	3646:3656	Rice starch has the highest apparent density among the three starches, indicating that rice starch molecules have a dense structure.
34677020	8	90	theme	starch	1402:1407	arg1	granules					1409:1416	starch granules	1402:1416	starch granules	1402:1416	The size detection range of a commercial AF4 system ranges from 1 nm to 10 μm, which is smaller than the size range of starch granules.
34677020	19	91	theme	dispersed	2955:2963	arg1	necessary					2977:2985	necessary	2977:2985	necessary	2977:2985	A molecularly dispersed solution is necessary for the reliable molecular characterization of starch.
34677020	19	91	theme	dispersed	2955:2963	arg1	solution					2965:2972	A molecularly dispersed solution	2941:2972	A molecularly dispersed solution	2941:2972	A molecularly dispersed solution is necessary for the reliable molecular characterization of starch.
34677020	14	92	theme	carrier	2103:2109	arg1	composition					2084:2094	the composition	2080:2094	the composition of the carrier liquid	2080:2116	The effect of the composition of the carrier liquid on the AF4 separation of starch granules was also studied.
34677020	15	93	theme	granules	2255:2262	arg1	characterization					2224:2239	size characterization	2219:2239	size characterization of the starch granules	2219:2262	Moreover, the accuracy of AF4 in terms of size characterization of the starch granules was evaluated by optical microscopy (OM).
34677020	26	94	theme	AF4	3976:3978	arg1	systems					3980:3986	commercial AF4 systems	3965:3986	commercial AF4 systems coupled online with MALS and dRI detectors	3965:4029	The developed AF4 system, when combined with commercial AF4 systems coupled online with MALS and dRI detectors, can provide technical support to study the relationship between the size from the nanoscale to the microscale and functional properties of starch.
34677020	19	95	theme	starch	3034:3039	arg1	characterization					3014:3029	the reliable molecular characterization	2991:3029	the reliable molecular characterization of starch	2991:3039	A molecularly dispersed solution is necessary for the reliable molecular characterization of starch.
34677020	8	96	theme	detection	1292:1300	arg1	range					1302:1306	The size detection range	1283:1306	The size detection range of a commercial AF4 system	1283:1333	The size detection range of a commercial AF4 system ranges from 1 nm to 10 μm, which is smaller than the size range of starch granules.
34677020	1	97	theme	carbohydrate	217:228	arg1	molecules					230:238	carbohydrate molecules	217:238	carbohydrate molecules	217:238	Starch occurs naturally in the form of semicrystalline granules, and is composed of two types of carbohydrate molecules, amylose (AM) and amylopectin (AP).
34677020	24	98	theme	highest	3666:3672	arg1	density					3683:3689	the highest apparent density	3662:3689	the highest apparent density among the three starches	3662:3714	Rice starch has the highest apparent density among the three starches, indicating that rice starch molecules have a dense structure.
34677020	9	99	theme	AF4	1446:1448	arg1	system					1450:1455	a home-made AF4 system	1434:1455	a home-made AF4 system	1434:1455	In this study, a home-made AF4 system was developed, and its capability for the size characterization of starch granules extracted from sweet potato, lotus seed, and rice was investigated.
34677020	17	100	theme	liquid	2741:2746	arg1	carrier					2733:2739	the carrier liquid	2729:2746	the carrier liquid	2729:2746	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	15	101	theme	characterization	2224:2239	arg1	terms					2210:2214	terms	2210:2214	terms of size characterization of the starch granules	2210:2262	Moreover, the accuracy of AF4 in terms of size characterization of the starch granules was evaluated by optical microscopy (OM).
34677020	21	102	theme	starch	3266:3271	arg1	granules					3273:3280	sweet potato starch granules	3253:3280	sweet potato starch granules	3253:3280	The optimal dissolution temperature for lotus seed and rice starch granules was 75 ℃, while that for sweet potato starch granules was 78 ℃; this difference is mainly attributed to the different botanical origins of the granules.
34677020	18	103	theme	molecules	2930:2938	arg1	characterization					2903:2918	characterization	2903:2918	characterization	2903:2918	Furthermore, a commercial AF4 system coupled with MALS and dRI detectors was employed for the separation and characterization of starch molecules.
34677020	18	103	theme	molecules	2930:2938	arg1	separation					2888:2897	separation	2888:2897	separation	2888:2897	Furthermore, a commercial AF4 system coupled with MALS and dRI detectors was employed for the separation and characterization of starch molecules.
34677020	18	104	theme	AF4	2820:2822	arg1	system					2824:2829	a commercial AF4 system	2807:2829	a commercial AF4 system coupled with MALS and dRI detectors	2807:2865	Furthermore, a commercial AF4 system coupled with MALS and dRI detectors was employed for the separation and characterization of starch molecules.
34677020	3	105	theme	flow	482:485	arg1	AF4					513:515	AF4	513:515	AF4	513:515	Asymmetrical flow field-flow fractionation (AF4) is a size-based separation technique.
34677020	3	105	theme	flow	482:485	arg1	technique					545:553	a size-based separation technique	521:553	a size-based separation technique	521:553	Asymmetrical flow field-flow fractionation (AF4) is a size-based separation technique.
34677020	3	105	theme	flow	482:485	arg1	fractionation					498:510	Asymmetrical flow field-flow fractionation	469:510	Asymmetrical flow field-flow fractionation (AF4)	469:516	Asymmetrical flow field-flow fractionation (AF4) is a size-based separation technique.
34677020	14	106	theme	starch	2143:2148	arg1	granules					2150:2157	starch granules	2143:2157	starch granules	2143:2157	The effect of the composition of the carrier liquid on the AF4 separation of starch granules was also studied.
34677020	11	107	theme	6	1835:1835	arg1	μm					1837:1838	μm	1837:1838	μm	1837:1838	Baseline separation of four PS samples was achieved, and the resolution for 6 μm PS and 12 μm PS was 1.40.
34677020	26	108	theme	AF4	3934:3936	arg1	system					3938:3943	The developed AF4 system	3920:3943	The developed AF4 system	3920:3943	The developed AF4 system, when combined with commercial AF4 systems coupled online with MALS and dRI detectors, can provide technical support to study the relationship between the size from the nanoscale to the microscale and functional properties of starch.
34677020	16	109	theme	granules	2450:2457	arg1	characterization					2419:2434	size characterization	2414:2434	size characterization of the starch granules	2414:2457	The results revealed that the type of dispersant and viscosity of the carrier liquid affect the accuracy of size characterization of the starch granules.
34677020	17	110	contain	containing	2538:2547	arg2	w/v					2662:2664	w/v	2662:2664	w/v	2662:2664	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	17	110	contain	containing	2538:2547	arg2	%					2659:2659	0.001%	2654:2659	0.001% (w/v)	2654:2665	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	17	110	contain	containing	2538:2547	arg2	sulfate					2576:2582	0.01% (w/v) sodium dodecyl sulfate	2549:2582	0.01% (w/v) sodium dodecyl sulfate (used as a dispersant)	2549:2605	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	17	110	contain	containing	2538:2547	arg2	NaN3					2620:2623	0.02% (w/v) NaN3	2608:2623	0.02% (w/v) NaN3 (used as a bactericide)	2608:2647	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	17	110	contain	containing	2538:2547	arg1	carrier					2523:2529	a carrier liquid	2521:2536	a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose	2521:2694	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	1	111	theme	semicrystalline	159:173	arg1	granules					175:182	semicrystalline granules	159:182	semicrystalline granules	159:182	Starch occurs naturally in the form of semicrystalline granules, and is composed of two types of carbohydrate molecules, amylose (AM) and amylopectin (AP).
34677020	14	112	from	effect	2070:2075	arg1	separation					2129:2138	the AF4 separation	2121:2138	the AF4 separation of starch granules	2121:2157	The effect of the composition of the carrier liquid on the AF4 separation of starch granules was also studied.
34677020	4	113	theme	dynamic	593:599	arg1	scattering					607:616	dynamic light scattering	593:616	dynamic light scattering	593:616	The major difference between AF4 and dynamic light scattering or microscopy techniques is that AF4 enables the separation of particles based on their size; consequently, the elution profile can be converted to the size distribution of the samples.
34677020	9	114	theme	lotus	1569:1573	arg1	seed					1575:1578	lotus seed	1569:1578	lotus seed	1569:1578	In this study, a home-made AF4 system was developed, and its capability for the size characterization of starch granules extracted from sweet potato, lotus seed, and rice was investigated.
34677020	5	115	from	level	1060:1064	arg1	characterization					1016:1031	the size characterization	1007:1031	the size characterization of starch at the molecular level	1007:1064	In the last two decades, AF4 systems, when coupled online with multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI), have demonstrated to be applicable for the size characterization of starch at the molecular level.
34677020	2	116	theme	Starch	276:281	arg1	granules					283:290	Starch granules	276:290	Starch granules	276:290	Starch granules and starch molecules have sizes in the range of 1-100 μm and 20-250 nm, respectively; these size ranges are among the key factors affecting the functional properties of starch.
34677020	20	117	theme	AF4	3098:3100	arg1	characterization					3102:3117	the AF4 characterization	3094:3117	the AF4 characterization of starch	3094:3127	The effect of the starch dissolution temperature on the AF4 characterization of starch was also investigated.
34677020	11	118	theme	12	1847:1848	arg1	μm					1850:1851	μm	1850:1851	μm	1850:1851	Baseline separation of four PS samples was achieved, and the resolution for 6 μm PS and 12 μm PS was 1.40.
34677020	24	119	theme	starch	3738:3743	arg1	molecules					3745:3753	rice starch molecules	3733:3753	rice starch molecules	3733:3753	Rice starch has the highest apparent density among the three starches, indicating that rice starch molecules have a dense structure.
34677020	11	120	theme	Baseline	1759:1766	arg1	separation					1768:1777	Baseline separation	1759:1777	Baseline separation of four PS samples	1759:1796	Baseline separation of four PS samples was achieved, and the resolution for 6 μm PS and 12 μm PS was 1.40.
34677020	15	121	theme	optical	2281:2287	arg1	OM					2301:2302	OM	2301:2302	OM	2301:2302	Moreover, the accuracy of AF4 in terms of size characterization of the starch granules was evaluated by optical microscopy (OM).
34677020	15	121	theme	optical	2281:2287	arg1	microscopy					2289:2298	optical microscopy	2281:2298	optical microscopy (OM)	2281:2303	Moreover, the accuracy of AF4 in terms of size characterization of the starch granules was evaluated by optical microscopy (OM).
34677020	13	122	theme	AF4	1982:1984	arg1	promising					1996:2004	promising	1996:2004	promising	1996:2004	Thus, the developed AF4 system is promising for the separation and characterization of starch granules.
34677020	13	122	theme	AF4	1982:1984	arg1	system					1986:1991	the developed AF4 system	1968:1991	the developed AF4 system	1968:1991	Thus, the developed AF4 system is promising for the separation and characterization of starch granules.
34677020	4	123	theme	elution	730:736	arg1	profile					738:744	the elution profile	726:744	the elution profile	726:744	The major difference between AF4 and dynamic light scattering or microscopy techniques is that AF4 enables the separation of particles based on their size; consequently, the elution profile can be converted to the size distribution of the samples.
34677020	9	124	theme	size	1499:1502	arg1	characterization					1504:1519	the size characterization	1495:1519	the size characterization of starch granules extracted from sweet potato, lotus seed, and rice	1495:1588	In this study, a home-made AF4 system was developed, and its capability for the size characterization of starch granules extracted from sweet potato, lotus seed, and rice was investigated.
34677020	22	125	theme	molar	3553:3557	arg1	range					3564:3568	the molar mass range	3549:3568	the molar mass range of 10 6-108 g/mol	3549:3586	The results showed that the ratio of the radius of gyration (Rg) to the hydrodynamic radius (Rh) of rice starch and sweet potato starch is in the range of 0.9-1.1 over the molar mass range of 10 6-108 g/mol.
34677020	21	126	theme	different	3336:3344	arg1	origins					3356:3362	the different botanical origins	3332:3362	the different botanical origins of the granules	3332:3378	The optimal dissolution temperature for lotus seed and rice starch granules was 75 ℃, while that for sweet potato starch granules was 78 ℃; this difference is mainly attributed to the different botanical origins of the granules.
34677020	3	127	theme	size-based	523:532	arg1	fractionation					498:510	Asymmetrical flow field-flow fractionation	469:510	Asymmetrical flow field-flow fractionation (AF4)	469:516	Asymmetrical flow field-flow fractionation (AF4) is a size-based separation technique.
34677020	3	127	theme	size-based	523:532	arg1	technique					545:553	a size-based separation technique	521:553	a size-based separation technique	521:553	Asymmetrical flow field-flow fractionation (AF4) is a size-based separation technique.
34677020	20	128	theme	dissolution	3067:3077	arg1	temperature					3079:3089	the starch dissolution temperature	3056:3089	the starch dissolution temperature	3056:3089	The effect of the starch dissolution temperature on the AF4 characterization of starch was also investigated.
34677020	11	129	theme	samples	1790:1796	arg1	separation					1768:1777	Baseline separation	1759:1777	Baseline separation of four PS samples	1759:1796	Baseline separation of four PS samples was achieved, and the resolution for 6 μm PS and 12 μm PS was 1.40.
34677020	8	130	theme	commercial	1313:1322	arg1	system					1328:1333	a commercial AF4 system	1311:1333	a commercial AF4 system	1311:1333	The size detection range of a commercial AF4 system ranges from 1 nm to 10 μm, which is smaller than the size range of starch granules.
34677020	6	131	theme	AF4	1111:1113	arg1	systems					1115:1121	AF4 systems	1111:1121	AF4 systems	1111:1121	Unlike size exclusion chromatography (SEC), AF4 systems use an open channel that does not require a stationary phase or packing materials.
34677020	5	132	theme	molecular	1050:1058	arg1	level					1060:1064	the molecular level	1046:1064	the molecular level	1046:1064	In the last two decades, AF4 systems, when coupled online with multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI), have demonstrated to be applicable for the size characterization of starch at the molecular level.
34677020	26	133	theme	MALS	4008:4011	arg1	detectors					4021:4029	MALS and dRI detectors	4008:4029	MALS and dRI detectors	4008:4029	The developed AF4 system, when combined with commercial AF4 systems coupled online with MALS and dRI detectors, can provide technical support to study the relationship between the size from the nanoscale to the microscale and functional properties of starch.
34677020	9	134	theme	granules	1531:1538	arg1	characterization					1504:1519	the size characterization	1495:1519	the size characterization of starch granules extracted from sweet potato, lotus seed, and rice	1495:1588	In this study, a home-made AF4 system was developed, and its capability for the size characterization of starch granules extracted from sweet potato, lotus seed, and rice was investigated.
34677020	8	135	theme	system	1328:1333	arg1	range					1302:1306	The size detection range	1283:1306	The size detection range of a commercial AF4 system	1283:1333	The size detection range of a commercial AF4 system ranges from 1 nm to 10 μm, which is smaller than the size range of starch granules.
34677020	7	136	theme	AP	1234:1235	arg1	molecules					1237:1245	AP molecules	1234:1245	AP molecules	1234:1245	Thus, the shear scission of AP molecules during AF4 separation is minimized.
34677020	6	137	theme	stationary	1167:1176	arg1	phase					1178:1182	a stationary phase	1165:1182	a stationary phase	1165:1182	Unlike size exclusion chromatography (SEC), AF4 systems use an open channel that does not require a stationary phase or packing materials.
34677020	10	138	theme	developed	1631:1639	arg1	system					1645:1650	the developed AF4 system	1627:1650	the developed AF4 system	1627:1650	The performance of the developed AF4 system was evaluated by running a mixture of polystyrene (PS) with diameter of 2, 6, 12, and 20 μm, respectively.
34677020	22	139	theme	mass	3559:3562	arg1	range					3564:3568	the molar mass range	3549:3568	the molar mass range of 10 6-108 g/mol	3549:3586	The results showed that the ratio of the radius of gyration (Rg) to the hydrodynamic radius (Rh) of rice starch and sweet potato starch is in the range of 0.9-1.1 over the molar mass range of 10 6-108 g/mol.
34677020	25	140	theme	starch	3903:3908	arg1	granules					3910:3917	starch granules	3903:3917	starch granules	3903:3917	The results demonstrated that the AF4 system developed in this study is rapid and accurate for the size characterization of starch granules.
34677020	16	141	theme	characterization	2419:2434	arg1	accuracy					2402:2409	the accuracy	2398:2409	the accuracy of size characterization of the starch granules	2398:2457	The results revealed that the type of dispersant and viscosity of the carrier liquid affect the accuracy of size characterization of the starch granules.
34677020	21	142	theme	dissolution	3164:3174	arg1	℃					3235:3235	75 ℃	3232:3235	75 ℃	3232:3235	The optimal dissolution temperature for lotus seed and rice starch granules was 75 ℃, while that for sweet potato starch granules was 78 ℃; this difference is mainly attributed to the different botanical origins of the granules.
34677020	21	142	theme	dissolution	3164:3174	arg1	temperature					3176:3186	The optimal dissolution temperature	3152:3186	The optimal dissolution temperature for lotus seed and rice starch granules	3152:3226	The optimal dissolution temperature for lotus seed and rice starch granules was 75 ℃, while that for sweet potato starch granules was 78 ℃; this difference is mainly attributed to the different botanical origins of the granules.
34677020	6	143	theme	exclusion	1079:1087	arg1	SEC					1105:1107	SEC	1105:1107	SEC	1105:1107	Unlike size exclusion chromatography (SEC), AF4 systems use an open channel that does not require a stationary phase or packing materials.
34677020	6	143	theme	exclusion	1079:1087	arg1	chromatography					1089:1102	size exclusion chromatography	1074:1102	size exclusion chromatography (SEC)	1074:1108	Unlike size exclusion chromatography (SEC), AF4 systems use an open channel that does not require a stationary phase or packing materials.
34677020	4	144	theme	microscopy	621:630	arg1	techniques					632:641	microscopy techniques	621:641	microscopy techniques	621:641	The major difference between AF4 and dynamic light scattering or microscopy techniques is that AF4 enables the separation of particles based on their size; consequently, the elution profile can be converted to the size distribution of the samples.
34677020	10	145	theme	μm	1741:1742	arg1	diameter					1712:1719	diameter	1712:1719	diameter of 2, 6, 12, and 20 μm	1712:1742	The performance of the developed AF4 system was evaluated by running a mixture of polystyrene (PS) with diameter of 2, 6, 12, and 20 μm, respectively.
34677020	5	146	theme	size	1011:1014	arg1	characterization					1016:1031	the size characterization	1007:1031	the size characterization of starch at the molecular level	1007:1064	In the last two decades, AF4 systems, when coupled online with multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI), have demonstrated to be applicable for the size characterization of starch at the molecular level.
34677020	2	147	theme	key	410:412	arg1	factors					414:420	the key factors	406:420	the key factors affecting the functional properties of starch	406:466	Starch granules and starch molecules have sizes in the range of 1-100 μm and 20-250 nm, respectively; these size ranges are among the key factors affecting the functional properties of starch.
34677020	22	148	theme	potato	3503:3508	arg1	starch					3510:3515	sweet potato starch	3497:3515	sweet potato starch	3497:3515	The results showed that the ratio of the radius of gyration (Rg) to the hydrodynamic radius (Rh) of rice starch and sweet potato starch is in the range of 0.9-1.1 over the molar mass range of 10 6-108 g/mol.
34677020	0	149	theme	starch	102:107	arg1	granules					109:116	starch granules	102:116	starch granules	102:116	[Development of an asymmetrical flow field-flow fractionation system for the size characterization of starch granules].
34677020	5	150	theme	multiangle	867:876	arg1	MALS					896:899	MALS	896:899	MALS	896:899	In the last two decades, AF4 systems, when coupled online with multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI), have demonstrated to be applicable for the size characterization of starch at the molecular level.
34677020	5	150	theme	multiangle	867:876	arg1	scattering					884:893	multiangle light scattering	867:893	multiangle light scattering (MALS)	867:900	In the last two decades, AF4 systems, when coupled online with multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI), have demonstrated to be applicable for the size characterization of starch at the molecular level.
34677020	12	151	theme	AF4	1949:1951	arg1	systems					1953:1959	commercial AF4 systems	1938:1959	commercial AF4 systems	1938:1959	The detection limit of the developed AF4 system was higher than that of commercial AF4 systems.
34677020	21	152	theme	rice	3207:3210	arg1	granules					3219:3226	rice starch granules	3207:3226	rice starch granules	3207:3226	The optimal dissolution temperature for lotus seed and rice starch granules was 75 ℃, while that for sweet potato starch granules was 78 ℃; this difference is mainly attributed to the different botanical origins of the granules.
34677020	5	153	theme	scattering	884:893	arg1	AF4-MALS-dRI					953:964	AF4-MALS-dRI	953:964	AF4-MALS-dRI	953:964	In the last two decades, AF4 systems, when coupled online with multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI), have demonstrated to be applicable for the size characterization of starch at the molecular level.
34677020	5	153	theme	scattering	884:893	arg1	detectors					942:950	multiangle light scattering (MALS) and differential refractive index (dRI) detectors	867:950	multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI)	867:965	In the last two decades, AF4 systems, when coupled online with multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI), have demonstrated to be applicable for the size characterization of starch at the molecular level.
34677020	2	154	theme	functional	436:445	arg1	properties					447:456	the functional properties	432:456	the functional properties of starch	432:466	Starch granules and starch molecules have sizes in the range of 1-100 μm and 20-250 nm, respectively; these size ranges are among the key factors affecting the functional properties of starch.
34677020	17	155	theme	size	2464:2467	arg1	distribution					2469:2480	The size distribution	2460:2480	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid)	2460:2747	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	22	156	theme	rice	3481:3484	arg1	starch					3486:3491	rice starch	3481:3491	rice starch	3481:3491	The results showed that the ratio of the radius of gyration (Rg) to the hydrodynamic radius (Rh) of rice starch and sweet potato starch is in the range of 0.9-1.1 over the molar mass range of 10 6-108 g/mol.
34677020	24	157	contain	has	3658:3660	arg2	density					3683:3689	the highest apparent density	3662:3689	the highest apparent density among the three starches	3662:3714	Rice starch has the highest apparent density among the three starches, indicating that rice starch molecules have a dense structure.
34677020	24	157	contain	has	3658:3660	arg1	starch					3651:3656	Rice starch	3646:3656	Rice starch	3646:3656	Rice starch has the highest apparent density among the three starches, indicating that rice starch molecules have a dense structure.
34677020	26	158	theme	functional	4146:4155	arg1	properties					4157:4166	the microscale and functional properties	4127:4166	the microscale and functional properties of starch	4127:4176	The developed AF4 system, when combined with commercial AF4 systems coupled online with MALS and dRI detectors, can provide technical support to study the relationship between the size from the nanoscale to the microscale and functional properties of starch.
34677020	18	159	theme	starch	2923:2928	arg1	molecules					2930:2938	starch molecules	2923:2938	starch molecules	2923:2938	Furthermore, a commercial AF4 system coupled with MALS and dRI detectors was employed for the separation and characterization of starch molecules.
34677020	12	160	theme	developed	1893:1901	arg1	system					1907:1912	the developed AF4 system	1889:1912	the developed AF4 system	1889:1912	The detection limit of the developed AF4 system was higher than that of commercial AF4 systems.
34677020	26	161	theme	dRI	4017:4019	arg1	detectors					4021:4029	MALS and dRI detectors	4008:4029	MALS and dRI detectors	4008:4029	The developed AF4 system, when combined with commercial AF4 systems coupled online with MALS and dRI detectors, can provide technical support to study the relationship between the size from the nanoscale to the microscale and functional properties of starch.
34677020	0	162	theme	asymmetrical	19:30	arg1	system					62:67	an asymmetrical flow field-flow fractionation system	16:67	an asymmetrical flow field-flow fractionation system for the size characterization of starch granules	16:116	[Development of an asymmetrical flow field-flow fractionation system for the size characterization of starch granules].
34677020	13	163	theme	starch	2049:2054	arg1	granules					2056:2063	starch granules	2049:2063	starch granules	2049:2063	Thus, the developed AF4 system is promising for the separation and characterization of starch granules.
34677020	26	164	theme	microscale	4131:4140	arg1	properties					4157:4166	the microscale and functional properties	4127:4166	the microscale and functional properties of starch	4127:4176	The developed AF4 system, when combined with commercial AF4 systems coupled online with MALS and dRI detectors, can provide technical support to study the relationship between the size from the nanoscale to the microscale and functional properties of starch.
34677020	16	165	theme	carrier	2376:2382	arg1	viscosity					2359:2367	viscosity	2359:2367	viscosity	2359:2367	The results revealed that the type of dispersant and viscosity of the carrier liquid affect the accuracy of size characterization of the starch granules.
34677020	16	165	theme	carrier	2376:2382	arg1	dispersant					2344:2353	dispersant	2344:2353	dispersant	2344:2353	The results revealed that the type of dispersant and viscosity of the carrier liquid affect the accuracy of size characterization of the starch granules.
34677020	8	166	theme	size	1388:1391	arg1	range					1393:1397	the size range	1384:1397	the size range of starch granules	1384:1416	The size detection range of a commercial AF4 system ranges from 1 nm to 10 μm, which is smaller than the size range of starch granules.
34677020	12	167	theme	system	1907:1912	arg1	limit					1880:1884	The detection limit	1866:1884	The detection limit of the developed AF4 system	1866:1912	The detection limit of the developed AF4 system was higher than that of commercial AF4 systems.
34677020	12	167	theme	system	1907:1912	arg1	higher					1918:1923	higher	1918:1923	higher	1918:1923	The detection limit of the developed AF4 system was higher than that of commercial AF4 systems.
34677020	2	168	theme	nm	360:361	arg1	range					331:335	the range	327:335	the range of 1-100 μm and 20-250 nm	327:361	Starch granules and starch molecules have sizes in the range of 1-100 μm and 20-250 nm, respectively; these size ranges are among the key factors affecting the functional properties of starch.
34677020	0	169	theme	field-flow	37:46	arg1	system					62:67	an asymmetrical flow field-flow fractionation system	16:67	an asymmetrical flow field-flow fractionation system for the size characterization of starch granules	16:116	[Development of an asymmetrical flow field-flow fractionation system for the size characterization of starch granules].
34677020	21	170	theme	potato	3259:3264	arg1	granules					3273:3280	sweet potato starch granules	3253:3280	sweet potato starch granules	3253:3280	The optimal dissolution temperature for lotus seed and rice starch granules was 75 ℃, while that for sweet potato starch granules was 78 ℃; this difference is mainly attributed to the different botanical origins of the granules.
34677020	5	171	theme	refractive	919:928	arg1	index					930:934	differential refractive index	906:934	differential refractive index (dRI)	906:940	In the last two decades, AF4 systems, when coupled online with multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI), have demonstrated to be applicable for the size characterization of starch at the molecular level.
34677020	5	171	theme	refractive	919:928	arg1	dRI					937:939	dRI	937:939	dRI	937:939	In the last two decades, AF4 systems, when coupled online with multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI), have demonstrated to be applicable for the size characterization of starch at the molecular level.
34677020	17	172	theme	%	2553:2553	arg1	sulfate					2576:2582	0.01% (w/v) sodium dodecyl sulfate	2549:2582	0.01% (w/v) sodium dodecyl sulfate (used as a dispersant)	2549:2605	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	10	173	theme	system	1645:1650	arg1	performance					1612:1622	The performance	1608:1622	The performance of the developed AF4 system	1608:1650	The performance of the developed AF4 system was evaluated by running a mixture of polystyrene (PS) with diameter of 2, 6, 12, and 20 μm, respectively.
34677020	12	174	theme	detection	1870:1878	arg1	limit					1880:1884	The detection limit	1866:1884	The detection limit of the developed AF4 system	1866:1912	The detection limit of the developed AF4 system was higher than that of commercial AF4 systems.
34677020	12	174	theme	detection	1870:1878	arg1	higher					1918:1923	higher	1918:1923	higher	1918:1923	The detection limit of the developed AF4 system was higher than that of commercial AF4 systems.
34677020	17	175	theme	starch	2490:2495	arg1	granules					2497:2504	rice starch granules	2485:2504	rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose	2485:2694	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	0	176	theme	system	62:67	arg1	[Development					0:11	[Development	0:11	[Development	0:11	[Development of an asymmetrical flow field-flow fractionation system for the size characterization of starch granules].
34677020	13	177	theme	granules	2056:2063	arg1	characterization					2029:2044	characterization	2029:2044	characterization	2029:2044	Thus, the developed AF4 system is promising for the separation and characterization of starch granules.
34677020	13	177	theme	granules	2056:2063	arg1	separation					2014:2023	separation	2014:2023	separation	2014:2023	Thus, the developed AF4 system is promising for the separation and characterization of starch granules.
34677020	17	178	dep	%	2553:2553	arg1	w/v					2556:2558	w/v	2556:2558	w/v	2556:2558	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	8	179	theme	granules	1409:1416	arg1	range					1393:1397	the size range	1384:1397	the size range of starch granules	1384:1416	The size detection range of a commercial AF4 system ranges from 1 nm to 10 μm, which is smaller than the size range of starch granules.
34677020	14	180	theme	liquid	2111:2116	arg1	carrier					2103:2109	the carrier liquid	2099:2116	the carrier liquid	2099:2116	The effect of the composition of the carrier liquid on the AF4 separation of starch granules was also studied.
34677020	2	181	theme	starch	296:301	arg1	molecules					303:311	starch molecules	296:311	starch molecules	296:311	Starch granules and starch molecules have sizes in the range of 1-100 μm and 20-250 nm, respectively; these size ranges are among the key factors affecting the functional properties of starch.
34677020	15	182	theme	starch	2248:2253	arg1	granules					2255:2262	the starch granules	2244:2262	the starch granules	2244:2262	Moreover, the accuracy of AF4 in terms of size characterization of the starch granules was evaluated by optical microscopy (OM).
34677020	5	183	theme	differential	906:917	arg1	index					930:934	differential refractive index	906:934	differential refractive index (dRI)	906:940	In the last two decades, AF4 systems, when coupled online with multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI), have demonstrated to be applicable for the size characterization of starch at the molecular level.
34677020	5	183	theme	differential	906:917	arg1	dRI					937:939	dRI	937:939	dRI	937:939	In the last two decades, AF4 systems, when coupled online with multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI), have demonstrated to be applicable for the size characterization of starch at the molecular level.
34677020	17	184	dep	sulfate	2576:2582	arg1	used					2585:2588	used	2585:2588	used as a dispersant	2585:2604	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	17	184	dep	sulfate	2576:2582	arg1	hydroxypropylmethylcellulose					2667:2694	hydroxypropylmethylcellulose	2667:2694	hydroxypropylmethylcellulose	2667:2694	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	24	185	theme	dense	3762:3766	arg1	structure					3768:3776	a dense structure	3760:3776	a dense structure	3760:3776	Rice starch has the highest apparent density among the three starches, indicating that rice starch molecules have a dense structure.
34677020	26	186	theme	commercial	3965:3974	arg1	systems					3980:3986	commercial AF4 systems	3965:3986	commercial AF4 systems coupled online with MALS and dRI detectors	3965:4029	The developed AF4 system, when combined with commercial AF4 systems coupled online with MALS and dRI detectors, can provide technical support to study the relationship between the size from the nanoscale to the microscale and functional properties of starch.
34677020	25	187	theme	AF4	3813:3815	arg1	system					3817:3822	the AF4 system	3809:3822	the AF4 system developed in this study	3809:3846	The results demonstrated that the AF4 system developed in this study is rapid and accurate for the size characterization of starch granules.
34677020	25	187	theme	AF4	3813:3815	arg1	rapid					3851:3855	rapid	3851:3855	rapid	3851:3855	The results demonstrated that the AF4 system developed in this study is rapid and accurate for the size characterization of starch granules.
34677020	9	188	theme	home-made	1436:1444	arg1	system					1450:1455	a home-made AF4 system	1434:1455	a home-made AF4 system	1434:1455	In this study, a home-made AF4 system was developed, and its capability for the size characterization of starch granules extracted from sweet potato, lotus seed, and rice was investigated.
34677020	4	189	theme	major	560:564	arg1	difference					566:575	The major difference	556:575	The major difference between AF4 and dynamic light scattering or microscopy techniques	556:641	The major difference between AF4 and dynamic light scattering or microscopy techniques is that AF4 enables the separation of particles based on their size; consequently, the elution profile can be converted to the size distribution of the samples.
34677020	22	190	theme	gyration	3432:3439	arg1	radius					3422:3427	the radius	3418:3427	the radius of gyration (Rg)	3418:3444	The results showed that the ratio of the radius of gyration (Rg) to the hydrodynamic radius (Rh) of rice starch and sweet potato starch is in the range of 0.9-1.1 over the molar mass range of 10 6-108 g/mol.
34677020	8	191	theme	size	1287:1290	arg1	range					1302:1306	The size detection range	1283:1306	The size detection range of a commercial AF4 system	1283:1333	The size detection range of a commercial AF4 system ranges from 1 nm to 10 μm, which is smaller than the size range of starch granules.
34677020	14	192	theme	composition	2084:2094	arg1	effect					2070:2075	The effect	2066:2075	The effect of the composition of the carrier liquid on the AF4 separation of starch granules	2066:2157	The effect of the composition of the carrier liquid on the AF4 separation of starch granules was also studied.
34677020	17	193	theme	liquid	2531:2536	arg1	carrier					2523:2529	a carrier liquid	2521:2536	a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose	2521:2694	The size distribution of rice starch granules obtained using a carrier liquid containing 0.01% (w/v) sodium dodecyl sulfate (used as a dispersant), 0.02% (w/v) NaN3 (used as a bactericide), and 0.001% (w/v) hydroxypropylmethylcellulose (used to adjust the viscosity of the carrier liquid) was in agreement with that obtained from OM.
34677020	15	194	theme	size	2219:2222	arg1	characterization					2224:2239	size characterization	2219:2239	size characterization of the starch granules	2219:2262	Moreover, the accuracy of AF4 in terms of size characterization of the starch granules was evaluated by optical microscopy (OM).
34677020	1	195	theme	molecules	230:238	arg1	amylopectin					258:268	amylopectin	258:268	amylopectin (AP)	258:273	Starch occurs naturally in the form of semicrystalline granules, and is composed of two types of carbohydrate molecules, amylose (AM) and amylopectin (AP).
34677020	1	195	theme	molecules	230:238	arg1	amylose					241:247	amylose	241:247	amylose (AM)	241:252	Starch occurs naturally in the form of semicrystalline granules, and is composed of two types of carbohydrate molecules, amylose (AM) and amylopectin (AP).
34677020	1	195	theme	molecules	230:238	arg1	types					208:212	two types	204:212	two types of carbohydrate molecules	204:238	Starch occurs naturally in the form of semicrystalline granules, and is composed of two types of carbohydrate molecules, amylose (AM) and amylopectin (AP).
33064121	6	0	theme	scientific	1367:1376	arg1	basis					1378:1382	a scientific basis	1365:1382	a scientific basis for using AGM as potential prebiotics beneficial for prevention or therapeutic treatment of T2DM	1365:1479	This study provides a scientific basis for using AGM as potential prebiotics beneficial for prevention or therapeutic treatment of T2DM.
33064121	5	1	theme	certain	1248:1254	arg1	SCFAs					1256:1260	certain SCFAs	1248:1260	certain SCFAs	1248:1260	It was speculated that the hypoglycemic activity of AGM is decided by not the molecular weight but the glycosidic bonds/monosaccharide composition of AGM, which might be structurally specific to the gut bacteria, and thus certain SCFAs and endogenous metabolites that are related to the occurrence and therapy of T2DM.
33064121	4	2	theme	similar	863:869	arg1	strength					871:878	similar strength	863:878	similar strength of hypoglycemic effects	863:902	It was found that AGM and its enzyme-degraded products, though with different molecular weights, had similar β-glycosidic bonds and monosaccharide compositions, exerted similar strength of hypoglycemic effects, and reinstated with a similar extent the disordered gut microbiota and the contents of SCFAs and endogenous metabolites.
33064121	5	3	theme	SCFAs	1256:1260	arg1	composition					1161:1171	the glycosidic bonds/monosaccharide composition	1125:1171	the glycosidic bonds/monosaccharide composition of AGM, which might be structurally specific to the gut bacteria, and thus certain SCFAs and endogenous metabolites that are related to the occurrence and therapy of T2DM	1125:1342	It was speculated that the hypoglycemic activity of AGM is decided by not the molecular weight but the glycosidic bonds/monosaccharide composition of AGM, which might be structurally specific to the gut bacteria, and thus certain SCFAs and endogenous metabolites that are related to the occurrence and therapy of T2DM.
33064121	4	4	theme	disordered	946:955	arg1	microbiota					961:970	the disordered gut microbiota	942:970	the disordered gut microbiota	942:970	It was found that AGM and its enzyme-degraded products, though with different molecular weights, had similar β-glycosidic bonds and monosaccharide compositions, exerted similar strength of hypoglycemic effects, and reinstated with a similar extent the disordered gut microbiota and the contents of SCFAs and endogenous metabolites.
33064121	5	5	theme	glycosidic	1129:1138	arg1	composition					1161:1171	the glycosidic bonds/monosaccharide composition	1125:1171	the glycosidic bonds/monosaccharide composition of AGM, which might be structurally specific to the gut bacteria, and thus certain SCFAs and endogenous metabolites that are related to the occurrence and therapy of T2DM	1125:1342	It was speculated that the hypoglycemic activity of AGM is decided by not the molecular weight but the glycosidic bonds/monosaccharide composition of AGM, which might be structurally specific to the gut bacteria, and thus certain SCFAs and endogenous metabolites that are related to the occurrence and therapy of T2DM.
33064121	4	6	theme	hypoglycemic	883:894	arg1	effects					896:902	hypoglycemic effects	883:902	hypoglycemic effects	883:902	It was found that AGM and its enzyme-degraded products, though with different molecular weights, had similar β-glycosidic bonds and monosaccharide compositions, exerted similar strength of hypoglycemic effects, and reinstated with a similar extent the disordered gut microbiota and the contents of SCFAs and endogenous metabolites.
33064121	4	7	theme	enzyme-degraded	724:738	arg1	products					740:747	its enzyme-degraded products	720:747	its enzyme-degraded products	720:747	It was found that AGM and its enzyme-degraded products, though with different molecular weights, had similar β-glycosidic bonds and monosaccharide compositions, exerted similar strength of hypoglycemic effects, and reinstated with a similar extent the disordered gut microbiota and the contents of SCFAs and endogenous metabolites.
33064121	1	8	theme	enzyme-degraded	241:255	arg1	products					257:264	its enzyme-degraded products	237:264	its enzyme-degraded products from Amorphophallus albus	237:290	In the present study, the hypoglycemic effects of glucomannan (AGM) and its enzyme-degraded products from Amorphophallus albus were investigated.
33064121	3	9	theme	gut	534:536	arg1	bacteria					538:545	gut bacteria	534:545	gut bacteria	534:545	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	3	10	theme	HFD-STZ-induced	459:473	arg1	mice					507:510	HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice	459:510	HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice	459:510	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	0	11	theme	type	106:109	arg1	mellitus					122:129	type 2 diabetes mellitus	106:129	type 2 diabetes mellitus in mice and potential mechanisms	106:162	Similar hypoglycemic effects of glucomannan and its enzyme degraded products from Amorphophallus albus on type 2 diabetes mellitus in mice and potential mechanisms.
33064121	1	12	from	albus	286:290	arg1	products					257:264	its enzyme-degraded products	237:264	its enzyme-degraded products from Amorphophallus albus	237:290	In the present study, the hypoglycemic effects of glucomannan (AGM) and its enzyme-degraded products from Amorphophallus albus were investigated.
33064121	1	12	from	albus	286:290	arg1	effects					204:210	the hypoglycemic effects	187:210	the hypoglycemic effects of glucomannan (AGM)	187:231	In the present study, the hypoglycemic effects of glucomannan (AGM) and its enzyme-degraded products from Amorphophallus albus were investigated.
33064121	3	13	theme	bacteria	538:545	arg1	diversity					521:529	the diversity	517:529	the diversity of gut bacteria	517:545	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	2	14	theme	AGM	408:410	arg1	products					396:403	β-glucanase-degraded products	375:403	β-glucanase-degraded products of AGM	375:410	Four degraded products were prepared through ultrafiltration of β-glucanase-degraded products of AGM.
33064121	0	15	theme	hypoglycemic	8:19	arg1	effects					21:27	Similar hypoglycemic effects	0:27	Similar hypoglycemic effects of glucomannan	0:42	Similar hypoglycemic effects of glucomannan and its enzyme degraded products from Amorphophallus albus on type 2 diabetes mellitus in mice and potential mechanisms.
33064121	5	16	theme	molecular	1104:1112	arg1	weight					1114:1119	not the molecular weight	1096:1119	not the molecular weight	1096:1119	It was speculated that the hypoglycemic activity of AGM is decided by not the molecular weight but the glycosidic bonds/monosaccharide composition of AGM, which might be structurally specific to the gut bacteria, and thus certain SCFAs and endogenous metabolites that are related to the occurrence and therapy of T2DM.
33064121	3	17	theme	rRNA	567:570	arg1	sequencing					577:586	16S rRNA gene sequencing	563:586	16S rRNA gene sequencing	563:586	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	5	18	theme	T2DM	1339:1342	arg1	therapy					1328:1334	therapy	1328:1334	therapy	1328:1334	It was speculated that the hypoglycemic activity of AGM is decided by not the molecular weight but the glycosidic bonds/monosaccharide composition of AGM, which might be structurally specific to the gut bacteria, and thus certain SCFAs and endogenous metabolites that are related to the occurrence and therapy of T2DM.
33064121	5	18	theme	T2DM	1339:1342	arg1	occurrence					1313:1322	occurrence	1313:1322	occurrence	1313:1322	It was speculated that the hypoglycemic activity of AGM is decided by not the molecular weight but the glycosidic bonds/monosaccharide composition of AGM, which might be structurally specific to the gut bacteria, and thus certain SCFAs and endogenous metabolites that are related to the occurrence and therapy of T2DM.
33064121	1	19	theme	present	172:178	arg1	study					180:184	the present study	168:184	the present study	168:184	In the present study, the hypoglycemic effects of glucomannan (AGM) and its enzyme-degraded products from Amorphophallus albus were investigated.
33064121	0	20	theme	Similar	0:6	arg1	effects					21:27	Similar hypoglycemic effects	0:27	Similar hypoglycemic effects of glucomannan	0:42	Similar hypoglycemic effects of glucomannan and its enzyme degraded products from Amorphophallus albus on type 2 diabetes mellitus in mice and potential mechanisms.
33064121	3	21	theme	gene	572:575	arg1	sequencing					577:586	16S rRNA gene sequencing	563:586	16S rRNA gene sequencing	563:586	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	2	22	theme	products	396:403	arg1	ultrafiltration					356:370	ultrafiltration	356:370	ultrafiltration of β-glucanase-degraded products of AGM	356:410	Four degraded products were prepared through ultrafiltration of β-glucanase-degraded products of AGM.
33064121	6	23	theme	beneficial	1422:1431	arg1	AGM					1394:1396	AGM	1394:1396	AGM	1394:1396	This study provides a scientific basis for using AGM as potential prebiotics beneficial for prevention or therapeutic treatment of T2DM.
33064121	6	23	theme	beneficial	1422:1431	arg1	prebiotics					1411:1420	potential prebiotics	1401:1420	potential prebiotics beneficial for prevention or therapeutic treatment of T2DM	1401:1479	This study provides a scientific basis for using AGM as potential prebiotics beneficial for prevention or therapeutic treatment of T2DM.
33064121	6	24	theme	therapeutic	1451:1461	arg1	treatment					1463:1471	therapeutic treatment	1451:1471	therapeutic treatment	1451:1471	This study provides a scientific basis for using AGM as potential prebiotics beneficial for prevention or therapeutic treatment of T2DM.
33064121	3	25	theme	mellitus	491:498	arg1	mice					507:510	HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice	459:510	HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice	459:510	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	0	26	theme	diabetes	113:120	arg1	mellitus					122:129	type 2 diabetes mellitus	106:129	type 2 diabetes mellitus in mice and potential mechanisms	106:162	Similar hypoglycemic effects of glucomannan and its enzyme degraded products from Amorphophallus albus on type 2 diabetes mellitus in mice and potential mechanisms.
33064121	4	27	theme	similar	927:933	arg1	extent					935:940	a similar extent	925:940	a similar extent	925:940	It was found that AGM and its enzyme-degraded products, though with different molecular weights, had similar β-glycosidic bonds and monosaccharide compositions, exerted similar strength of hypoglycemic effects, and reinstated with a similar extent the disordered gut microbiota and the contents of SCFAs and endogenous metabolites.
33064121	3	28	theme	16S	563:565	arg1	sequencing					577:586	16S rRNA gene sequencing	563:586	16S rRNA gene sequencing	563:586	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	6	29	theme	potential	1401:1409	arg1	AGM					1394:1396	AGM	1394:1396	AGM	1394:1396	This study provides a scientific basis for using AGM as potential prebiotics beneficial for prevention or therapeutic treatment of T2DM.
33064121	6	29	theme	potential	1401:1409	arg1	prebiotics					1411:1420	potential prebiotics	1401:1420	potential prebiotics beneficial for prevention or therapeutic treatment of T2DM	1401:1479	This study provides a scientific basis for using AGM as potential prebiotics beneficial for prevention or therapeutic treatment of T2DM.
33064121	5	30	theme	AGM	1078:1080	arg1	activity					1066:1073	the hypoglycemic activity	1049:1073	the hypoglycemic activity of AGM	1049:1080	It was speculated that the hypoglycemic activity of AGM is decided by not the molecular weight but the glycosidic bonds/monosaccharide composition of AGM, which might be structurally specific to the gut bacteria, and thus certain SCFAs and endogenous metabolites that are related to the occurrence and therapy of T2DM.
33064121	4	31	theme	β-glycosidic	803:814	arg1	bonds					816:820	similar β-glycosidic bonds	795:820	similar β-glycosidic bonds	795:820	It was found that AGM and its enzyme-degraded products, though with different molecular weights, had similar β-glycosidic bonds and monosaccharide compositions, exerted similar strength of hypoglycemic effects, and reinstated with a similar extent the disordered gut microbiota and the contents of SCFAs and endogenous metabolites.
33064121	6	32	theme	T2DM	1476:1479	arg1	treatment					1463:1471	therapeutic treatment	1451:1471	therapeutic treatment	1451:1471	This study provides a scientific basis for using AGM as potential prebiotics beneficial for prevention or therapeutic treatment of T2DM.
33064121	6	32	theme	T2DM	1476:1479	arg1	prevention					1437:1446	prevention	1437:1446	prevention	1437:1446	This study provides a scientific basis for using AGM as potential prebiotics beneficial for prevention or therapeutic treatment of T2DM.
33064121	1	33	theme	hypoglycemic	191:202	arg1	effects					204:210	the hypoglycemic effects	187:210	the hypoglycemic effects of glucomannan (AGM)	187:231	In the present study, the hypoglycemic effects of glucomannan (AGM) and its enzyme-degraded products from Amorphophallus albus were investigated.
33064121	0	34	theme	glucomannan	32:42	arg1	effects					21:27	Similar hypoglycemic effects	0:27	Similar hypoglycemic effects of glucomannan	0:42	Similar hypoglycemic effects of glucomannan and its enzyme degraded products from Amorphophallus albus on type 2 diabetes mellitus in mice and potential mechanisms.
33064121	0	34	theme	glucomannan	32:42	arg1	enzyme					52:57	its enzyme	48:57	its enzyme	48:57	Similar hypoglycemic effects of glucomannan and its enzyme degraded products from Amorphophallus albus on type 2 diabetes mellitus in mice and potential mechanisms.
33064121	4	35	theme	endogenous	1002:1011	arg1	metabolites					1013:1023	endogenous metabolites	1002:1023	endogenous metabolites	1002:1023	It was found that AGM and its enzyme-degraded products, though with different molecular weights, had similar β-glycosidic bonds and monosaccharide compositions, exerted similar strength of hypoglycemic effects, and reinstated with a similar extent the disordered gut microbiota and the contents of SCFAs and endogenous metabolites.
33064121	4	36	theme	SCFAs	992:996	arg1	microbiota					961:970	the disordered gut microbiota	942:970	the disordered gut microbiota	942:970	It was found that AGM and its enzyme-degraded products, though with different molecular weights, had similar β-glycosidic bonds and monosaccharide compositions, exerted similar strength of hypoglycemic effects, and reinstated with a similar extent the disordered gut microbiota and the contents of SCFAs and endogenous metabolites.
33064121	4	36	theme	SCFAs	992:996	arg1	contents					980:987	the contents	976:987	the contents of SCFAs and endogenous metabolites	976:1023	It was found that AGM and its enzyme-degraded products, though with different molecular weights, had similar β-glycosidic bonds and monosaccharide compositions, exerted similar strength of hypoglycemic effects, and reinstated with a similar extent the disordered gut microbiota and the contents of SCFAs and endogenous metabolites.
33064121	3	37	theme	fecal	593:597	arg1	SCFAs					624:628	SCFAs	624:628	SCFAs	624:628	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	3	37	theme	fecal	593:597	arg1	acids					617:621	the fecal short chain fatty acids	589:621	the fecal short chain fatty acids (SCFAs)	589:629	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	3	38	theme	diabetes	482:489	arg1	T2DM					501:504	T2DM	501:504	T2DM	501:504	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	3	38	theme	diabetes	482:489	arg1	mellitus					491:498	type 2 diabetes mellitus	475:498	HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice	459:510	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	4	39	theme	metabolites	1013:1023	arg1	microbiota					961:970	the disordered gut microbiota	942:970	the disordered gut microbiota	942:970	It was found that AGM and its enzyme-degraded products, though with different molecular weights, had similar β-glycosidic bonds and monosaccharide compositions, exerted similar strength of hypoglycemic effects, and reinstated with a similar extent the disordered gut microbiota and the contents of SCFAs and endogenous metabolites.
33064121	4	39	theme	metabolites	1013:1023	arg1	contents					980:987	the contents	976:987	the contents of SCFAs and endogenous metabolites	976:1023	It was found that AGM and its enzyme-degraded products, though with different molecular weights, had similar β-glycosidic bonds and monosaccharide compositions, exerted similar strength of hypoglycemic effects, and reinstated with a similar extent the disordered gut microbiota and the contents of SCFAs and endogenous metabolites.
33064121	5	40	theme	bonds/monosaccharide	1140:1159	arg1	composition					1161:1171	the glycosidic bonds/monosaccharide composition	1125:1171	the glycosidic bonds/monosaccharide composition of AGM, which might be structurally specific to the gut bacteria, and thus certain SCFAs and endogenous metabolites that are related to the occurrence and therapy of T2DM	1125:1342	It was speculated that the hypoglycemic activity of AGM is decided by not the molecular weight but the glycosidic bonds/monosaccharide composition of AGM, which might be structurally specific to the gut bacteria, and thus certain SCFAs and endogenous metabolites that are related to the occurrence and therapy of T2DM.
33064121	0	41	theme	potential	143:151	arg1	mechanisms					153:162	potential mechanisms	143:162	potential mechanisms	143:162	Similar hypoglycemic effects of glucomannan and its enzyme degraded products from Amorphophallus albus on type 2 diabetes mellitus in mice and potential mechanisms.
33064121	3	42	theme	hypoglycemic	417:428	arg1	activities					430:439	The hypoglycemic activities	413:439	The hypoglycemic activities	413:439	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	4	43	theme	effects	896:902	arg1	strength					871:878	similar strength	863:878	similar strength of hypoglycemic effects	863:902	It was found that AGM and its enzyme-degraded products, though with different molecular weights, had similar β-glycosidic bonds and monosaccharide compositions, exerted similar strength of hypoglycemic effects, and reinstated with a similar extent the disordered gut microbiota and the contents of SCFAs and endogenous metabolites.
33064121	4	44	theme	monosaccharide	826:839	arg1	compositions					841:852	monosaccharide compositions	826:852	monosaccharide compositions	826:852	It was found that AGM and its enzyme-degraded products, though with different molecular weights, had similar β-glycosidic bonds and monosaccharide compositions, exerted similar strength of hypoglycemic effects, and reinstated with a similar extent the disordered gut microbiota and the contents of SCFAs and endogenous metabolites.
33064121	5	45	theme	endogenous	1266:1275	arg1	metabolites					1277:1287	endogenous metabolites	1266:1287	endogenous metabolites	1266:1287	It was speculated that the hypoglycemic activity of AGM is decided by not the molecular weight but the glycosidic bonds/monosaccharide composition of AGM, which might be structurally specific to the gut bacteria, and thus certain SCFAs and endogenous metabolites that are related to the occurrence and therapy of T2DM.
33064121	5	46	theme	AGM	1176:1178	arg1	composition					1161:1171	the glycosidic bonds/monosaccharide composition	1125:1171	the glycosidic bonds/monosaccharide composition of AGM, which might be structurally specific to the gut bacteria, and thus certain SCFAs and endogenous metabolites that are related to the occurrence and therapy of T2DM	1125:1342	It was speculated that the hypoglycemic activity of AGM is decided by not the molecular weight but the glycosidic bonds/monosaccharide composition of AGM, which might be structurally specific to the gut bacteria, and thus certain SCFAs and endogenous metabolites that are related to the occurrence and therapy of T2DM.
33064121	3	47	theme	fatty	611:615	arg1	SCFAs					624:628	SCFAs	624:628	SCFAs	624:628	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	3	47	theme	fatty	611:615	arg1	acids					617:621	the fecal short chain fatty acids	589:621	the fecal short chain fatty acids (SCFAs)	589:629	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	4	48	theme	similar	795:801	arg1	bonds					816:820	similar β-glycosidic bonds	795:820	similar β-glycosidic bonds	795:820	It was found that AGM and its enzyme-degraded products, though with different molecular weights, had similar β-glycosidic bonds and monosaccharide compositions, exerted similar strength of hypoglycemic effects, and reinstated with a similar extent the disordered gut microbiota and the contents of SCFAs and endogenous metabolites.
33064121	5	49	theme	gut	1225:1227	arg1	bacteria					1229:1236	the gut bacteria	1221:1236	the gut bacteria	1221:1236	It was speculated that the hypoglycemic activity of AGM is decided by not the molecular weight but the glycosidic bonds/monosaccharide composition of AGM, which might be structurally specific to the gut bacteria, and thus certain SCFAs and endogenous metabolites that are related to the occurrence and therapy of T2DM.
33064121	4	50	theme	different	762:770	arg1	weights					782:788	different molecular weights	762:788	different molecular weights	762:788	It was found that AGM and its enzyme-degraded products, though with different molecular weights, had similar β-glycosidic bonds and monosaccharide compositions, exerted similar strength of hypoglycemic effects, and reinstated with a similar extent the disordered gut microbiota and the contents of SCFAs and endogenous metabolites.
33064121	3	51	theme	endogenous	635:644	arg1	metabolites					646:656	endogenous metabolites	635:656	endogenous metabolites	635:656	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	4	52	theme	molecular	772:780	arg1	weights					782:788	different molecular weights	762:788	different molecular weights	762:788	It was found that AGM and its enzyme-degraded products, though with different molecular weights, had similar β-glycosidic bonds and monosaccharide compositions, exerted similar strength of hypoglycemic effects, and reinstated with a similar extent the disordered gut microbiota and the contents of SCFAs and endogenous metabolites.
33064121	2	53	theme	degraded	316:323	arg1	products					325:332	Four degraded products	311:332	Four degraded products	311:332	Four degraded products were prepared through ultrafiltration of β-glucanase-degraded products of AGM.
33064121	5	54	theme	hypoglycemic	1053:1064	arg1	activity					1066:1073	the hypoglycemic activity	1049:1073	the hypoglycemic activity of AGM	1049:1080	It was speculated that the hypoglycemic activity of AGM is decided by not the molecular weight but the glycosidic bonds/monosaccharide composition of AGM, which might be structurally specific to the gut bacteria, and thus certain SCFAs and endogenous metabolites that are related to the occurrence and therapy of T2DM.
33064121	1	55	theme	glucomannan	215:225	arg1	products					257:264	its enzyme-degraded products	237:264	its enzyme-degraded products from Amorphophallus albus	237:290	In the present study, the hypoglycemic effects of glucomannan (AGM) and its enzyme-degraded products from Amorphophallus albus were investigated.
33064121	1	55	theme	glucomannan	215:225	arg1	effects					204:210	the hypoglycemic effects	187:210	the hypoglycemic effects of glucomannan (AGM)	187:231	In the present study, the hypoglycemic effects of glucomannan (AGM) and its enzyme-degraded products from Amorphophallus albus were investigated.
33064121	0	56	from	albus	97:101	arg1	products					68:75	products	68:75	products from Amorphophallus albus	68:101	Similar hypoglycemic effects of glucomannan and its enzyme degraded products from Amorphophallus albus on type 2 diabetes mellitus in mice and potential mechanisms.
33064121	3	57	theme	short	599:603	arg1	SCFAs					624:628	SCFAs	624:628	SCFAs	624:628	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	3	57	theme	short	599:603	arg1	acids					617:621	the fecal short chain fatty acids	589:621	the fecal short chain fatty acids (SCFAs)	589:629	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	5	58	dep	occurrence	1313:1322	arg1	the					1309:1311	the	1309:1311	the	1309:1311	It was speculated that the hypoglycemic activity of AGM is decided by not the molecular weight but the glycosidic bonds/monosaccharide composition of AGM, which might be structurally specific to the gut bacteria, and thus certain SCFAs and endogenous metabolites that are related to the occurrence and therapy of T2DM.
33064121	2	59	theme	β-glucanase-degraded	375:394	arg1	products					396:403	β-glucanase-degraded products	375:403	β-glucanase-degraded products of AGM	375:410	Four degraded products were prepared through ultrafiltration of β-glucanase-degraded products of AGM.
33064121	3	60	theme	type	475:478	arg1	T2DM					501:504	T2DM	501:504	T2DM	501:504	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	3	60	theme	type	475:478	arg1	mellitus					491:498	type 2 diabetes mellitus	475:498	HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice	459:510	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	0	61	from	mellitus	122:129	arg1	mice					134:137	mice	134:137	mice	134:137	Similar hypoglycemic effects of glucomannan and its enzyme degraded products from Amorphophallus albus on type 2 diabetes mellitus in mice and potential mechanisms.
33064121	0	61	from	mellitus	122:129	arg1	mechanisms					153:162	potential mechanisms	143:162	potential mechanisms	143:162	Similar hypoglycemic effects of glucomannan and its enzyme degraded products from Amorphophallus albus on type 2 diabetes mellitus in mice and potential mechanisms.
33064121	3	62	theme	chain	605:609	arg1	SCFAs					624:628	SCFAs	624:628	SCFAs	624:628	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	3	62	theme	chain	605:609	arg1	acids					617:621	the fecal short chain fatty acids	589:621	the fecal short chain fatty acids (SCFAs)	589:629	The hypoglycemic activities were evaluated in HFD-STZ-induced type 2 diabetes mellitus (T2DM) mice, and the diversity of gut bacteria was analyzed by 16S rRNA gene sequencing; the fecal short chain fatty acids (SCFAs) and endogenous metabolites were determined by UPLC-QTOF-MS/MS.
33064121	4	63	theme	gut	957:959	arg1	microbiota					961:970	the disordered gut microbiota	942:970	the disordered gut microbiota	942:970	It was found that AGM and its enzyme-degraded products, though with different molecular weights, had similar β-glycosidic bonds and monosaccharide compositions, exerted similar strength of hypoglycemic effects, and reinstated with a similar extent the disordered gut microbiota and the contents of SCFAs and endogenous metabolites.
32194123	2	0	theme	magnetic	514:521	arg1	products					523:530	The magnetic products	510:530	The magnetic products	510:530	The magnetic products were characterized by FE-SEM, EDX, XRD, FT-IR, TGA, and VSM techniques.
32194123	5	1	theme	Dy	1044:1045	arg1	efficiencies					1004:1015	the adsorption efficiencies	989:1015	the adsorption efficiencies of Nd (III), Tb (III), and Dy (III)	989:1051	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	5	1	theme	Dy	1044:1045	arg1	%					1094:1094	95.72, 96.17, and 99.44%	1071:1094	respectively 95.72, 96.17, and 99.44%	1058:1094	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	0	2	theme	magnetic	225:232	arg1	nanoparticles					234:246	Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles	207:246	Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles	207:246	Response surface methodology based on central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan microbiocomposite powder containing Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles: Batch and column studies.
32194123	5	3	theme	initial	910:916	arg1	concentration					918:930	initial concentration	910:930	initial concentration of 30 mg/L	910:941	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	8	4	theme	CA/CMC/Ni0.2Zn0.2Fe2.6O4	1514:1537	arg1	behavior					1498:1505	the adsorption behavior	1483:1505	the adsorption behavior of the CA/CMC/Ni0.2Zn0.2Fe2.6O4 towards Nd (III), Tb (III), and Dy (III)	1483:1578	Additionally, the adsorption behavior of the CA/CMC/Ni0.2Zn0.2Fe2.6O4 towards Nd (III), Tb (III), and Dy (III) was studied by using a fixed-bed column technique.
32194123	0	5	theme	earth	99:103	arg1	elements					105:112	rare earth elements	94:112	rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan	94:169	Response surface methodology based on central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan microbiocomposite powder containing Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles: Batch and column studies.
32194123	7	6	theme	0.2 M	1276:1280	arg1	HNO3					1282:1285	0.2 M HNO3	1276:1285	0.2 M HNO3	1276:1285	The desorption of the loaded ions was effectively carried out by 0.2 M HNO3, and the adsorbent was consecutively utilized with 2.54, 1.63, and 1.16% decrease in adsorption efficiency for Nd (III), Tb (III), and Dy (III), respectively, after the forth cycle.
32194123	2	7	theme	VSM	588:590	arg1	techniques					592:601	VSM techniques	588:601	VSM techniques	588:601	The magnetic products were characterized by FE-SEM, EDX, XRD, FT-IR, TGA, and VSM techniques.
32194123	5	8	theme	53 min	964:969	arg1	time					956:959	contact time	948:959	contact time of 53 min predicted by RSM	948:986	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	5	8	theme	53 min	964:969	arg1	concentration					918:930	initial concentration	910:930	initial concentration of 30 mg/L	910:941	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	5	8	theme	53 min	964:969	arg1	pH = 5.5					873:880	pH = 5.5	873:880	pH = 5.5	873:880	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	5	8	theme	53 min	964:969	arg1	dosage					893:898	adsorbent dosage	883:898	adsorbent dosage of 0.1 g	883:907	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	0	9	theme	rare	94:97	arg1	elements					105:112	rare earth elements	94:112	rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan	94:169	Response surface methodology based on central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan microbiocomposite powder containing Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles: Batch and column studies.
32194123	1	10	theme	simultaneous	441:452	arg1	adsorption					462:471	the simultaneous aqueous adsorption	437:471	the simultaneous aqueous adsorption of Nd (III), Tb (III), and Dy (III)	437:507	In this research paper, the utilization of the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4 (CA/CMC/Ni0.2Zn0.2Fe2.6O4) was investigated for the simultaneous aqueous adsorption of Nd (III), Tb (III), and Dy (III).
32194123	0	11	contain	containing	196:205	arg1	powder					189:194	powder	189:194	powder containing Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles	189:246	Response surface methodology based on central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan microbiocomposite powder containing Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles: Batch and column studies.
32194123	0	11	contain	containing	196:205	arg2	nanoparticles					234:246	Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles	207:246	Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles	207:246	Response surface methodology based on central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan microbiocomposite powder containing Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles: Batch and column studies.
32194123	1	12	theme	aqueous	454:460	arg1	adsorption					462:471	the simultaneous aqueous adsorption	437:471	the simultaneous aqueous adsorption of Nd (III), Tb (III), and Dy (III)	437:507	In this research paper, the utilization of the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4 (CA/CMC/Ni0.2Zn0.2Fe2.6O4) was investigated for the simultaneous aqueous adsorption of Nd (III), Tb (III), and Dy (III).
32194123	0	13	theme	column	259:264	arg1	studies					266:272	column studies	259:272	column studies	259:272	Response surface methodology based on central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan microbiocomposite powder containing Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles: Batch and column studies.
32194123	1	14	theme	Dy	500:501	arg1	adsorption					462:471	the simultaneous aqueous adsorption	437:471	the simultaneous aqueous adsorption of Nd (III), Tb (III), and Dy (III)	437:507	In this research paper, the utilization of the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4 (CA/CMC/Ni0.2Zn0.2Fe2.6O4) was investigated for the simultaneous aqueous adsorption of Nd (III), Tb (III), and Dy (III).
32194123	7	15	theme	loaded	1233:1238	arg1	ions					1240:1243	the loaded ions	1229:1243	the loaded ions	1229:1243	The desorption of the loaded ions was effectively carried out by 0.2 M HNO3, and the adsorbent was consecutively utilized with 2.54, 1.63, and 1.16% decrease in adsorption efficiency for Nd (III), Tb (III), and Dy (III), respectively, after the forth cycle.
32194123	5	16	theme	pH = 5.5	873:880	arg1	conditions					859:868	the conditions	855:868	the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM	855:986	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	0	17	theme	elements	105:112	arg1	adsorption					80:89	simultaneous adsorption	67:89	simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan	67:169	Response surface methodology based on central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan microbiocomposite powder containing Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles: Batch and column studies.
32194123	6	18	theme	pseudo-second-order	1177:1195	arg1	models					1203:1208	Freundlich and pseudo-second-order (PSO) models	1162:1208	Freundlich and pseudo-second-order (PSO) models	1162:1208	The isotherm and kinetic data were respectively fitted well with Freundlich and pseudo-second-order (PSO) models.
32194123	6	18	theme	pseudo-second-order	1177:1195	arg1	PSO					1198:1200	PSO	1198:1200	PSO	1198:1200	The isotherm and kinetic data were respectively fitted well with Freundlich and pseudo-second-order (PSO) models.
32194123	8	19	theme	fixed-bed	1603:1611	arg1	technique					1620:1628	a fixed-bed column technique	1601:1628	a fixed-bed column technique	1601:1628	Additionally, the adsorption behavior of the CA/CMC/Ni0.2Zn0.2Fe2.6O4 towards Nd (III), Tb (III), and Dy (III) was studied by using a fixed-bed column technique.
32194123	0	20	theme	surface	9:15	arg1	methodology					17:27	Response surface methodology	0:27	Response surface methodology based on central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan	0:169	Response surface methodology based on central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan microbiocomposite powder containing Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles: Batch and column studies.
32194123	7	21	theme	forth	1456:1460	arg1	cycle					1462:1466	the forth cycle	1452:1466	the forth cycle	1452:1466	The desorption of the loaded ions was effectively carried out by 0.2 M HNO3, and the adsorbent was consecutively utilized with 2.54, 1.63, and 1.16% decrease in adsorption efficiency for Nd (III), Tb (III), and Dy (III), respectively, after the forth cycle.
32194123	7	22	dep	decrease	1360:1367	arg1	%					1358:1358	%	1358:1358	%	1358:1358	The desorption of the loaded ions was effectively carried out by 0.2 M HNO3, and the adsorbent was consecutively utilized with 2.54, 1.63, and 1.16% decrease in adsorption efficiency for Nd (III), Tb (III), and Dy (III), respectively, after the forth cycle.
32194123	5	23	theme	adsorbent	883:891	arg1	dosage					893:898	adsorbent dosage	883:898	adsorbent dosage of 0.1 g	883:907	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	0	24	theme	Response	0:7	arg1	methodology					17:27	Response surface methodology	0:27	Response surface methodology based on central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan	0:169	Response surface methodology based on central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan microbiocomposite powder containing Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles: Batch and column studies.
32194123	6	25	theme	kinetic	1114:1120	arg1	data					1122:1125	The isotherm and kinetic data	1097:1125	data	1122:1125	The isotherm and kinetic data were respectively fitted well with Freundlich and pseudo-second-order (PSO) models.
32194123	6	26	theme	Freundlich	1162:1171	arg1	models					1203:1208	Freundlich and pseudo-second-order (PSO) models	1162:1208	Freundlich and pseudo-second-order (PSO) models	1162:1208	The isotherm and kinetic data were respectively fitted well with Freundlich and pseudo-second-order (PSO) models.
32194123	6	26	theme	Freundlich	1162:1171	arg1	PSO					1198:1200	PSO	1198:1200	PSO	1198:1200	The isotherm and kinetic data were respectively fitted well with Freundlich and pseudo-second-order (PSO) models.
32194123	5	27	theme	contact	948:954	arg1	time					956:959	contact time	948:959	contact time of 53 min predicted by RSM	948:986	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	5	28	theme	dosage	893:898	arg1	conditions					859:868	the conditions	855:868	the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM	855:986	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	1	29	theme	magnetic	322:329	arg1	CA/CMC/Ni0.2Zn0.2Fe2.6O4					390:413	CA/CMC/Ni0.2Zn0.2Fe2.6O4	390:413	CA/CMC/Ni0.2Zn0.2Fe2.6O4	390:413	In this research paper, the utilization of the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4 (CA/CMC/Ni0.2Zn0.2Fe2.6O4) was investigated for the simultaneous aqueous adsorption of Nd (III), Tb (III), and Dy (III).
32194123	1	29	theme	magnetic	322:329	arg1	chitosan/Ni0.2Zn0.2Fe2.6O4					362:387	the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4	318:387	the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4 (CA/CMC/Ni0.2Zn0.2Fe2.6O4)	318:414	In this research paper, the utilization of the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4 (CA/CMC/Ni0.2Zn0.2Fe2.6O4) was investigated for the simultaneous aqueous adsorption of Nd (III), Tb (III), and Dy (III).
32194123	0	30	theme	calcium	131:137	arg1	chitosan					162:169	nanoporous calcium alginate/carboxymethyl chitosan	120:169	nanoporous calcium alginate/carboxymethyl chitosan	120:169	Response surface methodology based on central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan microbiocomposite powder containing Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles: Batch and column studies.
32194123	8	31	theme	column	1613:1618	arg1	technique					1620:1628	a fixed-bed column technique	1601:1628	a fixed-bed column technique	1601:1628	Additionally, the adsorption behavior of the CA/CMC/Ni0.2Zn0.2Fe2.6O4 towards Nd (III), Tb (III), and Dy (III) was studied by using a fixed-bed column technique.
32194123	4	32	theme	polynomial	766:775	arg1	equation					777:784	a quadratic polynomial equation	754:784	a quadratic polynomial equation	754:784	Using RSM, a quadratic polynomial equation was obtained to predict the adsorption efficiency of each ion.
32194123	1	33	theme	calcium	331:337	arg1	CA/CMC/Ni0.2Zn0.2Fe2.6O4					390:413	CA/CMC/Ni0.2Zn0.2Fe2.6O4	390:413	CA/CMC/Ni0.2Zn0.2Fe2.6O4	390:413	In this research paper, the utilization of the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4 (CA/CMC/Ni0.2Zn0.2Fe2.6O4) was investigated for the simultaneous aqueous adsorption of Nd (III), Tb (III), and Dy (III).
32194123	1	33	theme	calcium	331:337	arg1	chitosan/Ni0.2Zn0.2Fe2.6O4					362:387	the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4	318:387	the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4 (CA/CMC/Ni0.2Zn0.2Fe2.6O4)	318:414	In this research paper, the utilization of the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4 (CA/CMC/Ni0.2Zn0.2Fe2.6O4) was investigated for the simultaneous aqueous adsorption of Nd (III), Tb (III), and Dy (III).
32194123	0	34	theme	nanoporous	120:129	arg1	chitosan					162:169	nanoporous calcium alginate/carboxymethyl chitosan	120:169	nanoporous calcium alginate/carboxymethyl chitosan	120:169	Response surface methodology based on central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan microbiocomposite powder containing Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles: Batch and column studies.
32194123	1	35	theme	Nd	476:477	arg1	adsorption					462:471	the simultaneous aqueous adsorption	437:471	the simultaneous aqueous adsorption of Nd (III), Tb (III), and Dy (III)	437:507	In this research paper, the utilization of the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4 (CA/CMC/Ni0.2Zn0.2Fe2.6O4) was investigated for the simultaneous aqueous adsorption of Nd (III), Tb (III), and Dy (III).
32194123	4	36	theme	quadratic	756:764	arg1	equation					777:784	a quadratic polynomial equation	754:784	a quadratic polynomial equation	754:784	Using RSM, a quadratic polynomial equation was obtained to predict the adsorption efficiency of each ion.
32194123	0	37	theme	central	38:44	arg1	design					56:61	central composite design	38:61	central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan	38:169	Response surface methodology based on central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan microbiocomposite powder containing Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles: Batch and column studies.
32194123	1	38	theme	alginate/carboxymethyl	339:360	arg1	CA/CMC/Ni0.2Zn0.2Fe2.6O4					390:413	CA/CMC/Ni0.2Zn0.2Fe2.6O4	390:413	CA/CMC/Ni0.2Zn0.2Fe2.6O4	390:413	In this research paper, the utilization of the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4 (CA/CMC/Ni0.2Zn0.2Fe2.6O4) was investigated for the simultaneous aqueous adsorption of Nd (III), Tb (III), and Dy (III).
32194123	1	38	theme	alginate/carboxymethyl	339:360	arg1	chitosan/Ni0.2Zn0.2Fe2.6O4					362:387	the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4	318:387	the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4 (CA/CMC/Ni0.2Zn0.2Fe2.6O4)	318:414	In this research paper, the utilization of the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4 (CA/CMC/Ni0.2Zn0.2Fe2.6O4) was investigated for the simultaneous aqueous adsorption of Nd (III), Tb (III), and Dy (III).
32194123	7	39	theme	ions	1240:1243	arg1	desorption					1215:1224	The desorption	1211:1224	The desorption of the loaded ions	1211:1243	The desorption of the loaded ions was effectively carried out by 0.2 M HNO3, and the adsorbent was consecutively utilized with 2.54, 1.63, and 1.16% decrease in adsorption efficiency for Nd (III), Tb (III), and Dy (III), respectively, after the forth cycle.
32194123	7	40	used	utilized	1324:1331	arg2	adsorbent					1296:1304	adsorbent	1296:1304	adsorbent	1296:1304	The desorption of the loaded ions was effectively carried out by 0.2 M HNO3, and the adsorbent was consecutively utilized with 2.54, 1.63, and 1.16% decrease in adsorption efficiency for Nd (III), Tb (III), and Dy (III), respectively, after the forth cycle.
32194123	1	41	theme	chitosan/Ni0.2Zn0.2Fe2.6O4	362:387	arg1	utilization					303:313	the utilization	299:313	the utilization of the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4 (CA/CMC/Ni0.2Zn0.2Fe2.6O4)	299:414	In this research paper, the utilization of the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4 (CA/CMC/Ni0.2Zn0.2Fe2.6O4) was investigated for the simultaneous aqueous adsorption of Nd (III), Tb (III), and Dy (III).
32194123	0	42	theme	alginate/carboxymethyl	139:160	arg1	chitosan					162:169	nanoporous calcium alginate/carboxymethyl chitosan	120:169	nanoporous calcium alginate/carboxymethyl chitosan	120:169	Response surface methodology based on central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan microbiocomposite powder containing Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles: Batch and column studies.
32194123	5	43	theme	Nd	1020:1021	arg1	efficiencies					1004:1015	the adsorption efficiencies	989:1015	the adsorption efficiencies of Nd (III), Tb (III), and Dy (III)	989:1051	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	5	43	theme	Nd	1020:1021	arg1	%					1094:1094	95.72, 96.17, and 99.44%	1071:1094	respectively 95.72, 96.17, and 99.44%	1058:1094	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	7	44	dep	%	1358:1358	arg1	1.16					1354:1357	1.16	1354:1357	1.16	1354:1357	The desorption of the loaded ions was effectively carried out by 0.2 M HNO3, and the adsorbent was consecutively utilized with 2.54, 1.63, and 1.16% decrease in adsorption efficiency for Nd (III), Tb (III), and Dy (III), respectively, after the forth cycle.
32194123	7	44	dep	%	1358:1358	arg1	1.63					1344:1347	1.63	1344:1347	1.63	1344:1347	The desorption of the loaded ions was effectively carried out by 0.2 M HNO3, and the adsorbent was consecutively utilized with 2.54, 1.63, and 1.16% decrease in adsorption efficiency for Nd (III), Tb (III), and Dy (III), respectively, after the forth cycle.
32194123	7	45	from	decrease	1360:1367	arg1	efficiency					1383:1392	adsorption efficiency	1372:1392	adsorption efficiency for Nd (III), Tb (III), and Dy (III), respectively, after the forth cycle	1372:1466	The desorption of the loaded ions was effectively carried out by 0.2 M HNO3, and the adsorbent was consecutively utilized with 2.54, 1.63, and 1.16% decrease in adsorption efficiency for Nd (III), Tb (III), and Dy (III), respectively, after the forth cycle.
32194123	5	46	theme	concentration	918:930	arg1	conditions					859:868	the conditions	855:868	the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM	855:986	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	0	47	theme	composite	46:54	arg1	design					56:61	central composite design	38:61	central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan	38:169	Response surface methodology based on central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan microbiocomposite powder containing Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles: Batch and column studies.
32194123	4	48	theme	ion	844:846	arg1	efficiency					825:834	the adsorption efficiency	810:834	the adsorption efficiency of each ion	810:846	Using RSM, a quadratic polynomial equation was obtained to predict the adsorption efficiency of each ion.
32194123	4	49	theme	adsorption	814:823	arg1	efficiency					825:834	the adsorption efficiency	810:834	the adsorption efficiency of each ion	810:846	Using RSM, a quadratic polynomial equation was obtained to predict the adsorption efficiency of each ion.
32194123	0	50	theme	Ni0.2Zn0.2Fe2.6O4	207:223	arg1	nanoparticles					234:246	Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles	207:246	Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles	207:246	Response surface methodology based on central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan microbiocomposite powder containing Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles: Batch and column studies.
32194123	1	51	theme	Tb	486:487	arg1	adsorption					462:471	the simultaneous aqueous adsorption	437:471	the simultaneous aqueous adsorption of Nd (III), Tb (III), and Dy (III)	437:507	In this research paper, the utilization of the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4 (CA/CMC/Ni0.2Zn0.2Fe2.6O4) was investigated for the simultaneous aqueous adsorption of Nd (III), Tb (III), and Dy (III).
32194123	3	52	theme	magnetization	619:631	arg1	45.87					706:710	45.87	706:710	45.87	706:710	The saturation magnetization value for Ni0.2Zn0.2Fe2.6O4 and CA/CMC/Ni0.2Zn0.2Fe2.6O4 was found to be 45.87 and 14.14 emu/g, respectively.
32194123	3	52	theme	magnetization	619:631	arg1	value					633:637	The saturation magnetization value	604:637	The saturation magnetization value for Ni0.2Zn0.2Fe2.6O4 and CA/CMC/Ni0.2Zn0.2Fe2.6O4	604:688	The saturation magnetization value for Ni0.2Zn0.2Fe2.6O4 and CA/CMC/Ni0.2Zn0.2Fe2.6O4 was found to be 45.87 and 14.14 emu/g, respectively.
32194123	5	53	theme	30 mg/L	935:941	arg1	time					956:959	contact time	948:959	contact time of 53 min predicted by RSM	948:986	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	5	53	theme	30 mg/L	935:941	arg1	concentration					918:930	initial concentration	910:930	initial concentration of 30 mg/L	910:941	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	5	53	theme	30 mg/L	935:941	arg1	pH = 5.5					873:880	pH = 5.5	873:880	pH = 5.5	873:880	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	5	53	theme	30 mg/L	935:941	arg1	dosage					893:898	adsorbent dosage	883:898	adsorbent dosage of 0.1 g	883:907	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	5	54	theme	time	956:959	arg1	conditions					859:868	the conditions	855:868	the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM	855:986	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	5	55	theme	Tb	1030:1031	arg1	efficiencies					1004:1015	the adsorption efficiencies	989:1015	the adsorption efficiencies of Nd (III), Tb (III), and Dy (III)	989:1051	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	5	55	theme	Tb	1030:1031	arg1	%					1094:1094	95.72, 96.17, and 99.44%	1071:1094	respectively 95.72, 96.17, and 99.44%	1058:1094	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	6	56	theme	isotherm	1101:1108	arg1	data					1122:1125	The isotherm and kinetic data	1097:1125	data	1122:1125	The isotherm and kinetic data were respectively fitted well with Freundlich and pseudo-second-order (PSO) models.
32194123	0	57	dep	microbiocomposite	171:187	arg1	Batch					249:253	Batch	249:253	Batch	249:253	Response surface methodology based on central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan microbiocomposite powder containing Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles: Batch and column studies.
32194123	0	57	dep	microbiocomposite	171:187	arg1	studies					266:272	column studies	259:272	column studies	259:272	Response surface methodology based on central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan microbiocomposite powder containing Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles: Batch and column studies.
32194123	8	58	theme	adsorption	1487:1496	arg1	behavior					1498:1505	the adsorption behavior	1483:1505	the adsorption behavior of the CA/CMC/Ni0.2Zn0.2Fe2.6O4 towards Nd (III), Tb (III), and Dy (III)	1483:1578	Additionally, the adsorption behavior of the CA/CMC/Ni0.2Zn0.2Fe2.6O4 towards Nd (III), Tb (III), and Dy (III) was studied by using a fixed-bed column technique.
32194123	0	59	theme	simultaneous	67:78	arg1	adsorption					80:89	simultaneous adsorption	67:89	simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan	67:169	Response surface methodology based on central composite design for simultaneous adsorption of rare earth elements using nanoporous calcium alginate/carboxymethyl chitosan microbiocomposite powder containing Ni0.2Zn0.2Fe2.6O4 magnetic nanoparticles: Batch and column studies.
32194123	5	60	theme	adsorption	993:1002	arg1	efficiencies					1004:1015	the adsorption efficiencies	989:1015	the adsorption efficiencies of Nd (III), Tb (III), and Dy (III)	989:1051	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	5	60	theme	adsorption	993:1002	arg1	%					1094:1094	95.72, 96.17, and 99.44%	1071:1094	respectively 95.72, 96.17, and 99.44%	1058:1094	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	1	61	theme	research	283:290	arg1	paper					292:296	this research paper	278:296	this research paper	278:296	In this research paper, the utilization of the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4 (CA/CMC/Ni0.2Zn0.2Fe2.6O4) was investigated for the simultaneous aqueous adsorption of Nd (III), Tb (III), and Dy (III).
32194123	7	62	theme	adsorption	1372:1381	arg1	efficiency					1383:1392	adsorption efficiency	1372:1392	adsorption efficiency for Nd (III), Tb (III), and Dy (III), respectively, after the forth cycle	1372:1466	The desorption of the loaded ions was effectively carried out by 0.2 M HNO3, and the adsorbent was consecutively utilized with 2.54, 1.63, and 1.16% decrease in adsorption efficiency for Nd (III), Tb (III), and Dy (III), respectively, after the forth cycle.
32194123	3	63	theme	saturation	608:617	arg1	45.87					706:710	45.87	706:710	45.87	706:710	The saturation magnetization value for Ni0.2Zn0.2Fe2.6O4 and CA/CMC/Ni0.2Zn0.2Fe2.6O4 was found to be 45.87 and 14.14 emu/g, respectively.
32194123	3	63	theme	saturation	608:617	arg1	value					633:637	The saturation magnetization value	604:637	The saturation magnetization value for Ni0.2Zn0.2Fe2.6O4 and CA/CMC/Ni0.2Zn0.2Fe2.6O4	604:688	The saturation magnetization value for Ni0.2Zn0.2Fe2.6O4 and CA/CMC/Ni0.2Zn0.2Fe2.6O4 was found to be 45.87 and 14.14 emu/g, respectively.
32194123	5	64	theme	0.1 g	903:907	arg1	time					956:959	contact time	948:959	contact time of 53 min predicted by RSM	948:986	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	5	64	theme	0.1 g	903:907	arg1	concentration					918:930	initial concentration	910:930	initial concentration of 30 mg/L	910:941	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	5	64	theme	0.1 g	903:907	arg1	pH = 5.5					873:880	pH = 5.5	873:880	pH = 5.5	873:880	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
32194123	5	64	theme	0.1 g	903:907	arg1	dosage					893:898	adsorbent dosage	883:898	adsorbent dosage of 0.1 g	883:907	Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%.
34237372	10	0	theme	films	957:961	arg1	Cytotoxicity					937:948	Cytotoxicity	937:948	Cytotoxicity of the films	937:961	Cytotoxicity of the films decreased after loading with BNNPs.
34237372	5	1	theme	pure	507:510	arg1	film					515:518	pure CS film	507:518	pure CS film to 2.9 × 10-11 g m-1s-1Pa-1 at 7 wt% BNNP inclusion	507:570	Additionally, water vapor permeation decreased from 4.2 × 10-11 for pure CS film to 2.9 × 10-11 g m-1s-1Pa-1 at 7 wt% BNNP inclusion.
34237372	5	2	from	inclusion	562:570	arg1	film					515:518	pure CS film	507:518	pure CS film to 2.9 × 10-11 g m-1s-1Pa-1 at 7 wt% BNNP inclusion	507:570	Additionally, water vapor permeation decreased from 4.2 × 10-11 for pure CS film to 2.9 × 10-11 g m-1s-1Pa-1 at 7 wt% BNNP inclusion.
34237372	6	3	theme	CS	600:601	arg1	film					603:606	CS film	600:606	CS film	600:606	The oxygen permeability of CS film decreased by up to 84% at 7 wt% BNNP loading.
34237372	0	4	theme	packaging	87:95	arg1	applications					97:108	active food packaging applications	75:108	active food packaging applications	75:108	Chitosan/boron nitride nanobiocomposite films with improved properties for active food packaging applications.
34237372	1	5	theme	nanobiocomposite	159:174	arg1	films					176:180	Chitosan (CS)/boron nitride nanoplatelet (BNNP) nanobiocomposite films	111:180	Chitosan (CS)/boron nitride nanoplatelet (BNNP) nanobiocomposite films	111:180	Chitosan (CS)/boron nitride nanoplatelet (BNNP) nanobiocomposite films were successfully prepared.
34237372	7	6	theme	better	676:681	arg1	resistance					700:709	better sodium hydroxide resistance	676:709	better sodium hydroxide resistance	676:709	The composites showed better sodium hydroxide resistance compared with pure CS.
34237372	8	7	dep	35 °C	805:809	arg1	to					802:803	to	802:803	to	802:803	Thermal stability of the composites was higher than the pure CS, up to 35 °C increase at 7 wt% BNNP loading.
34237372	6	8	dep	84	627:628	arg1	to					624:625	to	624:625	to	624:625	The oxygen permeability of CS film decreased by up to 84% at 7 wt% BNNP loading.
34237372	5	9	theme	CS	512:513	arg1	film					515:518	pure CS film	507:518	pure CS film to 2.9 × 10-11 g m-1s-1Pa-1 at 7 wt% BNNP inclusion	507:570	Additionally, water vapor permeation decreased from 4.2 × 10-11 for pure CS film to 2.9 × 10-11 g m-1s-1Pa-1 at 7 wt% BNNP inclusion.
34237372	9	10	theme	5 wt	859:862	arg1	BNNPs					865:869	5 wt% BNNPs	859:869	5 wt% BNNPs	859:869	The addition of 5 wt% BNNPs improved Young's modulus by up to 45% compared with pure CS film.
34237372	5	11	theme	water	453:457	arg1	permeation					465:474	water vapor permeation	453:474	water vapor permeation	453:474	Additionally, water vapor permeation decreased from 4.2 × 10-11 for pure CS film to 2.9 × 10-11 g m-1s-1Pa-1 at 7 wt% BNNP inclusion.
34237372	9	12	theme	CS	928:929	arg1	film					931:934	pure CS film	923:934	pure CS film	923:934	The addition of 5 wt% BNNPs improved Young's modulus by up to 45% compared with pure CS film.
34237372	3	13	theme	moisture	334:341	arg1	absorption					343:352	water solubility (WS) and moisture absorption	308:352	absorption	343:352	After loading with BNNPs, water solubility (WS) and moisture absorption of the CS film decreased.
34237372	5	14	theme	vapor	459:463	arg1	permeation					465:474	water vapor permeation	453:474	water vapor permeation	453:474	Additionally, water vapor permeation decreased from 4.2 × 10-11 for pure CS film to 2.9 × 10-11 g m-1s-1Pa-1 at 7 wt% BNNP inclusion.
34237372	9	15	theme	BNNPs	865:869	arg1	addition					847:854	The addition	843:854	The addition of 5 wt% BNNPs	843:869	The addition of 5 wt% BNNPs improved Young's modulus by up to 45% compared with pure CS film.
34237372	9	16	dep	45	905:906	arg1	to					902:903	to	902:903	to	902:903	The addition of 5 wt% BNNPs improved Young's modulus by up to 45% compared with pure CS film.
34237372	8	17	dep	increase	811:818	arg1	higher					774:779	higher	774:779	higher	774:779	Thermal stability of the composites was higher than the pure CS, up to 35 °C increase at 7 wt% BNNP loading.
34237372	8	17	dep	increase	811:818	arg1	35 °C					805:809	35 °C	805:809	35 °C	805:809	Thermal stability of the composites was higher than the pure CS, up to 35 °C increase at 7 wt% BNNP loading.
34237372	9	18	theme	Young	880:884	arg1	modulus					888:894	Young's modulus	880:894	Young's modulus	880:894	The addition of 5 wt% BNNPs improved Young's modulus by up to 45% compared with pure CS film.
34237372	6	19	theme	BNNP	640:643	arg1	loading					645:651	7 wt% BNNP loading	634:651	7 wt% BNNP loading	634:651	The oxygen permeability of CS film decreased by up to 84% at 7 wt% BNNP loading.
34237372	9	20	theme	%	863:863	arg1	BNNPs					865:869	5 wt% BNNPs	859:869	5 wt% BNNPs	859:869	The addition of 5 wt% BNNPs improved Young's modulus by up to 45% compared with pure CS film.
34237372	0	21	theme	nitride	15:21	arg1	films					40:44	Chitosan/boron nitride nanobiocomposite films	0:44	Chitosan/boron nitride nanobiocomposite films with improved properties for active food packaging applications	0:108	Chitosan/boron nitride nanobiocomposite films with improved properties for active food packaging applications.
34237372	5	22	theme	7 wt	551:554	arg1	inclusion					562:570	7 wt% BNNP inclusion	551:570	7 wt% BNNP inclusion	551:570	Additionally, water vapor permeation decreased from 4.2 × 10-11 for pure CS film to 2.9 × 10-11 g m-1s-1Pa-1 at 7 wt% BNNP inclusion.
34237372	6	23	theme	%	638:638	arg1	loading					645:651	7 wt% BNNP loading	634:651	7 wt% BNNP loading	634:651	The oxygen permeability of CS film decreased by up to 84% at 7 wt% BNNP loading.
34237372	0	24	theme	Chitosan/boron	0:13	arg1	films					40:44	Chitosan/boron nitride nanobiocomposite films	0:44	Chitosan/boron nitride nanobiocomposite films with improved properties for active food packaging applications	0:108	Chitosan/boron nitride nanobiocomposite films with improved properties for active food packaging applications.
34237372	5	25	theme	%	555:555	arg1	inclusion					562:570	7 wt% BNNP inclusion	551:570	7 wt% BNNP inclusion	551:570	Additionally, water vapor permeation decreased from 4.2 × 10-11 for pure CS film to 2.9 × 10-11 g m-1s-1Pa-1 at 7 wt% BNNP inclusion.
34237372	1	26	theme	Chitosan	111:118	arg1	films					176:180	Chitosan (CS)/boron nitride nanoplatelet (BNNP) nanobiocomposite films	111:180	Chitosan (CS)/boron nitride nanoplatelet (BNNP) nanobiocomposite films	111:180	Chitosan (CS)/boron nitride nanoplatelet (BNNP) nanobiocomposite films were successfully prepared.
34237372	6	27	theme	7 wt	634:637	arg1	loading					645:651	7 wt% BNNP loading	634:651	7 wt% BNNP loading	634:651	The oxygen permeability of CS film decreased by up to 84% at 7 wt% BNNP loading.
34237372	8	28	from	loading	834:840	arg1	increase					811:818	increase	811:818	increase	811:818	Thermal stability of the composites was higher than the pure CS, up to 35 °C increase at 7 wt% BNNP loading.
34237372	3	29	theme	CS	361:362	arg1	film					364:367	the CS film	357:367	the CS film	357:367	After loading with BNNPs, water solubility (WS) and moisture absorption of the CS film decreased.
34237372	5	30	theme	BNNP	557:560	arg1	inclusion					562:570	7 wt% BNNP inclusion	551:570	7 wt% BNNP inclusion	551:570	Additionally, water vapor permeation decreased from 4.2 × 10-11 for pure CS film to 2.9 × 10-11 g m-1s-1Pa-1 at 7 wt% BNNP inclusion.
34237372	10	31	with	loading	979:985	arg1	BNNPs					992:996	BNNPs	992:996	BNNPs	992:996	Cytotoxicity of the films decreased after loading with BNNPs.
34237372	3	32	theme	film	364:367	arg1	absorption					343:352	water solubility (WS) and moisture absorption	308:352	absorption	343:352	After loading with BNNPs, water solubility (WS) and moisture absorption of the CS film decreased.
34237372	3	32	theme	film	364:367	arg1	WS					326:327	water solubility (WS) and moisture absorption	308:352	WS	326:327	After loading with BNNPs, water solubility (WS) and moisture absorption of the CS film decreased.
34237372	4	33	theme	7 wt	419:422	arg1	loading					430:436	7 wt% BNNP loading	419:436	7 wt% BNNP loading	419:436	The WS decreased from 41.2 to 27.8% at 7 wt% BNNP loading.
34237372	8	34	theme	BNNP	829:832	arg1	loading					834:840	7 wt% BNNP loading	823:840	7 wt% BNNP loading	823:840	Thermal stability of the composites was higher than the pure CS, up to 35 °C increase at 7 wt% BNNP loading.
34237372	8	35	theme	7 wt	823:826	arg1	BNNP					829:832	7 wt% BNNP	823:832	7 wt% BNNP loading	823:840	Thermal stability of the composites was higher than the pure CS, up to 35 °C increase at 7 wt% BNNP loading.
34237372	0	36	theme	improved	51:58	arg1	properties					60:69	improved properties	51:69	improved properties	51:69	Chitosan/boron nitride nanobiocomposite films with improved properties for active food packaging applications.
34237372	2	37	theme	BNNPs	258:262	arg1	dispersion					244:253	good dispersion	239:253	good dispersion of BNNPs	239:262	Morphological results showed good dispersion of BNNPs in the CS matrix.
34237372	8	38	theme	%	827:827	arg1	BNNP					829:832	7 wt% BNNP	823:832	7 wt% BNNP loading	823:840	Thermal stability of the composites was higher than the pure CS, up to 35 °C increase at 7 wt% BNNP loading.
34237372	3	39	with	loading	288:294	arg1	BNNPs					301:305	BNNPs	301:305	BNNPs	301:305	After loading with BNNPs, water solubility (WS) and moisture absorption of the CS film decreased.
34237372	8	40	theme	Thermal	734:740	arg1	stability					742:750	Thermal stability	734:750	Thermal stability of the composites	734:768	Thermal stability of the composites was higher than the pure CS, up to 35 °C increase at 7 wt% BNNP loading.
34237372	4	41	theme	BNNP	425:428	arg1	loading					430:436	7 wt% BNNP loading	419:436	7 wt% BNNP loading	419:436	The WS decreased from 41.2 to 27.8% at 7 wt% BNNP loading.
34237372	0	42	with	films	40:44	arg1	properties					60:69	improved properties	51:69	improved properties	51:69	Chitosan/boron nitride nanobiocomposite films with improved properties for active food packaging applications.
34237372	3	43	theme	water	308:312	arg1	WS					326:327	water solubility (WS) and moisture absorption	308:352	WS	326:327	After loading with BNNPs, water solubility (WS) and moisture absorption of the CS film decreased.
34237372	4	44	theme	%	423:423	arg1	loading					430:436	7 wt% BNNP loading	419:436	7 wt% BNNP loading	419:436	The WS decreased from 41.2 to 27.8% at 7 wt% BNNP loading.
34237372	6	45	theme	oxygen	577:582	arg1	permeability					584:595	The oxygen permeability	573:595	The oxygen permeability of CS film	573:606	The oxygen permeability of CS film decreased by up to 84% at 7 wt% BNNP loading.
34237372	3	46	theme	solubility	314:323	arg1	WS					326:327	water solubility (WS) and moisture absorption	308:352	WS	326:327	After loading with BNNPs, water solubility (WS) and moisture absorption of the CS film decreased.
34237372	2	47	theme	good	239:242	arg1	dispersion					244:253	good dispersion	239:253	good dispersion of BNNPs	239:262	Morphological results showed good dispersion of BNNPs in the CS matrix.
34237372	1	48	theme	/boron	124:129	arg1	films					176:180	Chitosan (CS)/boron nitride nanoplatelet (BNNP) nanobiocomposite films	111:180	Chitosan (CS)/boron nitride nanoplatelet (BNNP) nanobiocomposite films	111:180	Chitosan (CS)/boron nitride nanoplatelet (BNNP) nanobiocomposite films were successfully prepared.
34237372	7	49	theme	pure	725:728	arg1	CS					730:731	pure CS	725:731	pure CS	725:731	The composites showed better sodium hydroxide resistance compared with pure CS.
34237372	1	50	theme	nitride	131:137	arg1	films					176:180	Chitosan (CS)/boron nitride nanoplatelet (BNNP) nanobiocomposite films	111:180	Chitosan (CS)/boron nitride nanoplatelet (BNNP) nanobiocomposite films	111:180	Chitosan (CS)/boron nitride nanoplatelet (BNNP) nanobiocomposite films were successfully prepared.
34237372	8	51	theme	composites	759:768	arg1	stability					742:750	Thermal stability	734:750	Thermal stability of the composites	734:768	Thermal stability of the composites was higher than the pure CS, up to 35 °C increase at 7 wt% BNNP loading.
34237372	2	52	theme	Morphological	210:222	arg1	results					224:230	Morphological results	210:230	Morphological results	210:230	Morphological results showed good dispersion of BNNPs in the CS matrix.
34237372	2	53	theme	CS	271:272	arg1	matrix					274:279	the CS matrix	267:279	the CS matrix	267:279	Morphological results showed good dispersion of BNNPs in the CS matrix.
34237372	1	54	theme	nanoplatelet	139:150	arg1	films					176:180	Chitosan (CS)/boron nitride nanoplatelet (BNNP) nanobiocomposite films	111:180	Chitosan (CS)/boron nitride nanoplatelet (BNNP) nanobiocomposite films	111:180	Chitosan (CS)/boron nitride nanoplatelet (BNNP) nanobiocomposite films were successfully prepared.
34237372	4	55	dep	27.8	410:413	arg1	to					407:408	to	407:408	to	407:408	The WS decreased from 41.2 to 27.8% at 7 wt% BNNP loading.
34237372	0	56	theme	food	82:85	arg1	applications					97:108	active food packaging applications	75:108	active food packaging applications	75:108	Chitosan/boron nitride nanobiocomposite films with improved properties for active food packaging applications.
34237372	8	57	theme	pure	790:793	arg1	CS					795:796	the pure CS	786:796	the pure CS	786:796	Thermal stability of the composites was higher than the pure CS, up to 35 °C increase at 7 wt% BNNP loading.
34237372	9	58	theme	pure	923:926	arg1	film					931:934	pure CS film	923:934	pure CS film	923:934	The addition of 5 wt% BNNPs improved Young's modulus by up to 45% compared with pure CS film.
34237372	7	59	theme	sodium	683:688	arg1	resistance					700:709	better sodium hydroxide resistance	676:709	better sodium hydroxide resistance	676:709	The composites showed better sodium hydroxide resistance compared with pure CS.
34237372	0	60	theme	active	75:80	arg1	applications					97:108	active food packaging applications	75:108	active food packaging applications	75:108	Chitosan/boron nitride nanobiocomposite films with improved properties for active food packaging applications.
34237372	0	61	theme	nanobiocomposite	23:38	arg1	films					40:44	Chitosan/boron nitride nanobiocomposite films	0:44	Chitosan/boron nitride nanobiocomposite films with improved properties for active food packaging applications	0:108	Chitosan/boron nitride nanobiocomposite films with improved properties for active food packaging applications.
34237372	1	62	theme	BNNP	153:156	arg1	films					176:180	Chitosan (CS)/boron nitride nanoplatelet (BNNP) nanobiocomposite films	111:180	Chitosan (CS)/boron nitride nanoplatelet (BNNP) nanobiocomposite films	111:180	Chitosan (CS)/boron nitride nanoplatelet (BNNP) nanobiocomposite films were successfully prepared.
34237372	6	63	theme	film	603:606	arg1	permeability					584:595	The oxygen permeability	573:595	The oxygen permeability of CS film	573:606	The oxygen permeability of CS film decreased by up to 84% at 7 wt% BNNP loading.
34237372	7	64	theme	hydroxide	690:698	arg1	resistance					700:709	better sodium hydroxide resistance	676:709	better sodium hydroxide resistance	676:709	The composites showed better sodium hydroxide resistance compared with pure CS.
33309658	0	0	theme	molecular	82:90	arg1	weight					92:97	different molecular weight	72:97	different molecular weight on gelatin-based composite hydrogels	72:134	Investigation on the tunable effect of oxidized konjac glucomannan with different molecular weight on gelatin-based composite hydrogels.
33309658	1	1	with	glucomannan	161:171	arg1	weight					204:209	different molecular weight	184:209	different molecular weight (Mw)	184:214	Herein, oxidized konjac glucomannan (OKG) with different molecular weight (Mw) were prepared as polysaccharide crosslinker to reinforce gelatin-based hydrogels.
33309658	1	1	with	glucomannan	161:171	arg1	Mw					212:213	Mw	212:213	Mw	212:213	Herein, oxidized konjac glucomannan (OKG) with different molecular weight (Mw) were prepared as polysaccharide crosslinker to reinforce gelatin-based hydrogels.
33309658	6	2	theme	hydrogels	1082:1090	arg1	properties					1054:1063	the overall properties	1042:1063	the overall properties of gelatin-based hydrogels	1042:1090	In brief, this work provides a facile method to promote the overall properties of gelatin-based hydrogels, meanwhile revealed the relationship and mechanism underlying the effects of OKG with different Mw on composite hydrogels.
33309658	1	3	theme	polysaccharide	233:246	arg1	crosslinker					248:258	polysaccharide crosslinker	233:258	polysaccharide crosslinker	233:258	Herein, oxidized konjac glucomannan (OKG) with different molecular weight (Mw) were prepared as polysaccharide crosslinker to reinforce gelatin-based hydrogels.
33309658	1	3	theme	polysaccharide	233:246	arg1	glucomannan					161:171	oxidized konjac glucomannan	145:171	oxidized konjac glucomannan (OKG) with different molecular weight (Mw)	145:214	Herein, oxidized konjac glucomannan (OKG) with different molecular weight (Mw) were prepared as polysaccharide crosslinker to reinforce gelatin-based hydrogels.
33309658	0	4	theme	different	72:80	arg1	weight					92:97	different molecular weight	72:97	different molecular weight on gelatin-based composite hydrogels	72:134	Investigation on the tunable effect of oxidized konjac glucomannan with different molecular weight on gelatin-based composite hydrogels.
33309658	6	5	theme	gelatin-based	1068:1080	arg1	hydrogels					1082:1090	gelatin-based hydrogels	1068:1090	gelatin-based hydrogels	1068:1090	In brief, this work provides a facile method to promote the overall properties of gelatin-based hydrogels, meanwhile revealed the relationship and mechanism underlying the effects of OKG with different Mw on composite hydrogels.
33309658	5	6	theme	well	874:877	arg1	proliferation					879:891	well proliferation	874:891	well proliferation of cells, which showed excellent capacity in tissue engineering and biomedical applications	874:983	Furthermore, both composite hydrogels could support well proliferation of cells, which showed excellent capacity in tissue engineering and biomedical applications.
33309658	0	7	from	Investigation	0:12	arg1	effect					29:34	the tunable effect	17:34	the tunable effect of oxidized konjac	17:53	Investigation on the tunable effect of oxidized konjac glucomannan with different molecular weight on gelatin-based composite hydrogels.
33309658	4	8	theme	same	765:768	arg1	time					770:773	the same time	761:773	the same time	761:773	Besides, composite hydrogels not only showed increased mechanical strength, but self-healing ability at the same time, which were closely affected by the Mw of OKG.
33309658	5	9	theme	tissue	938:943	arg1	engineering					945:955	tissue engineering	938:955	tissue engineering	938:955	Furthermore, both composite hydrogels could support well proliferation of cells, which showed excellent capacity in tissue engineering and biomedical applications.
33309658	2	10	theme	mechanical	503:512	arg1	spectroscopy					436:447	spectroscopy	436:447	spectroscopy (FTIR)	436:454	Then, properties of composite hydrogels with various OKGs were investigated via a series of methods, including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests.
33309658	2	10	theme	mechanical	503:512	arg1	tests					535:539	mechanical and biocompatibility tests	503:539	mechanical and biocompatibility tests	503:539	Then, properties of composite hydrogels with various OKGs were investigated via a series of methods, including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests.
33309658	6	11	theme	composite	1194:1202	arg1	hydrogels					1204:1212	composite hydrogels	1194:1212	composite hydrogels	1194:1212	In brief, this work provides a facile method to promote the overall properties of gelatin-based hydrogels, meanwhile revealed the relationship and mechanism underlying the effects of OKG with different Mw on composite hydrogels.
33309658	0	12	theme	composite	116:124	arg1	hydrogels					126:134	gelatin-based composite hydrogels	102:134	gelatin-based composite hydrogels	102:134	Investigation on the tunable effect of oxidized konjac glucomannan with different molecular weight on gelatin-based composite hydrogels.
33309658	5	13	theme	cells	896:900	arg1	proliferation					879:891	well proliferation	874:891	well proliferation of cells, which showed excellent capacity in tissue engineering and biomedical applications	874:983	Furthermore, both composite hydrogels could support well proliferation of cells, which showed excellent capacity in tissue engineering and biomedical applications.
33309658	4	14	theme	self-healing	737:748	arg1	ability					750:756	self-healing ability	737:756	self-healing ability	737:756	Besides, composite hydrogels not only showed increased mechanical strength, but self-healing ability at the same time, which were closely affected by the Mw of OKG.
33309658	5	15	theme	biomedical	961:970	arg1	applications					972:983	biomedical applications	961:983	biomedical applications	961:983	Furthermore, both composite hydrogels could support well proliferation of cells, which showed excellent capacity in tissue engineering and biomedical applications.
33309658	6	16	theme	overall	1046:1052	arg1	properties					1054:1063	the overall properties	1042:1063	the overall properties of gelatin-based hydrogels	1042:1090	In brief, this work provides a facile method to promote the overall properties of gelatin-based hydrogels, meanwhile revealed the relationship and mechanism underlying the effects of OKG with different Mw on composite hydrogels.
33309658	0	17	theme	gelatin-based	102:114	arg1	hydrogels					126:134	gelatin-based composite hydrogels	102:134	gelatin-based composite hydrogels	102:134	Investigation on the tunable effect of oxidized konjac glucomannan with different molecular weight on gelatin-based composite hydrogels.
33309658	4	18	theme	OKG	817:819	arg1	Mw					811:812	the Mw	807:812	the Mw of OKG	807:819	Besides, composite hydrogels not only showed increased mechanical strength, but self-healing ability at the same time, which were closely affected by the Mw of OKG.
33309658	5	19	theme	excellent	916:924	arg1	capacity					926:933	excellent capacity	916:933	excellent capacity	916:933	Furthermore, both composite hydrogels could support well proliferation of cells, which showed excellent capacity in tissue engineering and biomedical applications.
33309658	2	20	theme	methods	390:396	arg1	series					380:385	a series	378:385	a series of methods, including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests	378:539	Then, properties of composite hydrogels with various OKGs were investigated via a series of methods, including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests.
33309658	6	21	dep	provides	1006:1013	arg1	revealed					1103:1110	revealed	1103:1110	revealed the relationship and mechanism underlying the effects of OKG with different Mw on composite hydrogels	1103:1212	In brief, this work provides a facile method to promote the overall properties of gelatin-based hydrogels, meanwhile revealed the relationship and mechanism underlying the effects of OKG with different Mw on composite hydrogels.
33309658	6	22	with	OKG	1169:1171	arg1	Mw					1188:1189	different Mw	1178:1189	different Mw	1178:1189	In brief, this work provides a facile method to promote the overall properties of gelatin-based hydrogels, meanwhile revealed the relationship and mechanism underlying the effects of OKG with different Mw on composite hydrogels.
33309658	2	23	theme	composite	318:326	arg1	hydrogels					328:336	composite hydrogels	318:336	composite hydrogels with various OKGs	318:354	Then, properties of composite hydrogels with various OKGs were investigated via a series of methods, including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests.
33309658	4	24	theme	composite	666:674	arg1	hydrogels					676:684	composite hydrogels	666:684	composite hydrogels	666:684	Besides, composite hydrogels not only showed increased mechanical strength, but self-healing ability at the same time, which were closely affected by the Mw of OKG.
33309658	1	25	theme	gelatin-based	273:285	arg1	hydrogels					287:295	gelatin-based hydrogels	273:295	gelatin-based hydrogels	273:295	Herein, oxidized konjac glucomannan (OKG) with different molecular weight (Mw) were prepared as polysaccharide crosslinker to reinforce gelatin-based hydrogels.
33309658	3	26	theme	increased	567:575	arg1	degree					577:582	an increased degree	564:582	an increased degree of crosslinking	564:598	The results confirmed an increased degree of crosslinking and entanglement between gelatin and OKG with higher Mw.
33309658	1	27	theme	different	184:192	arg1	weight					204:209	different molecular weight	184:209	different molecular weight (Mw)	184:214	Herein, oxidized konjac glucomannan (OKG) with different molecular weight (Mw) were prepared as polysaccharide crosslinker to reinforce gelatin-based hydrogels.
33309658	1	27	theme	different	184:192	arg1	Mw					212:213	Mw	212:213	Mw	212:213	Herein, oxidized konjac glucomannan (OKG) with different molecular weight (Mw) were prepared as polysaccharide crosslinker to reinforce gelatin-based hydrogels.
33309658	0	28	theme	tunable	21:27	arg1	effect					29:34	the tunable effect	17:34	the tunable effect of oxidized konjac	17:53	Investigation on the tunable effect of oxidized konjac glucomannan with different molecular weight on gelatin-based composite hydrogels.
33309658	3	29	theme	crosslinking	587:598	arg1	degree					577:582	an increased degree	564:582	an increased degree of crosslinking	564:598	The results confirmed an increased degree of crosslinking and entanglement between gelatin and OKG with higher Mw.
33309658	1	30	theme	molecular	194:202	arg1	weight					204:209	different molecular weight	184:209	different molecular weight (Mw)	184:214	Herein, oxidized konjac glucomannan (OKG) with different molecular weight (Mw) were prepared as polysaccharide crosslinker to reinforce gelatin-based hydrogels.
33309658	1	30	theme	molecular	194:202	arg1	Mw					212:213	Mw	212:213	Mw	212:213	Herein, oxidized konjac glucomannan (OKG) with different molecular weight (Mw) were prepared as polysaccharide crosslinker to reinforce gelatin-based hydrogels.
33309658	6	31	theme	different	1178:1186	arg1	Mw					1188:1189	different Mw	1178:1189	different Mw	1178:1189	In brief, this work provides a facile method to promote the overall properties of gelatin-based hydrogels, meanwhile revealed the relationship and mechanism underlying the effects of OKG with different Mw on composite hydrogels.
33309658	6	32	theme	OKG	1169:1171	arg1	effects					1158:1164	the effects	1154:1164	the effects of OKG with different Mw on composite hydrogels	1154:1212	In brief, this work provides a facile method to promote the overall properties of gelatin-based hydrogels, meanwhile revealed the relationship and mechanism underlying the effects of OKG with different Mw on composite hydrogels.
33309658	3	33	theme	higher	646:651	arg1	Mw					653:654	higher Mw	646:654	higher Mw	646:654	The results confirmed an increased degree of crosslinking and entanglement between gelatin and OKG with higher Mw.
33309658	4	34	dep	increased	702:710	arg1	affected					795:802	affected	795:802	were closely affected by the Mw of OKG	782:819	Besides, composite hydrogels not only showed increased mechanical strength, but self-healing ability at the same time, which were closely affected by the Mw of OKG.
33309658	6	35	from	effects	1158:1164	arg1	hydrogels					1204:1212	composite hydrogels	1194:1212	composite hydrogels	1194:1212	In brief, this work provides a facile method to promote the overall properties of gelatin-based hydrogels, meanwhile revealed the relationship and mechanism underlying the effects of OKG with different Mw on composite hydrogels.
33309658	2	36	with	hydrogels	328:336	arg1	OKGs					351:354	various OKGs	343:354	various OKGs	343:354	Then, properties of composite hydrogels with various OKGs were investigated via a series of methods, including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests.
33309658	2	37	dep	Fourier	409:415	arg1	transform					417:425	transform	417:425	transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests	417:539	Then, properties of composite hydrogels with various OKGs were investigated via a series of methods, including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests.
33309658	2	38	theme	electron	466:473	arg1	spectroscopy					436:447	spectroscopy	436:447	spectroscopy (FTIR)	436:454	Then, properties of composite hydrogels with various OKGs were investigated via a series of methods, including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests.
33309658	2	38	theme	electron	466:473	arg1	SEM					487:489	SEM	487:489	SEM	487:489	Then, properties of composite hydrogels with various OKGs were investigated via a series of methods, including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests.
33309658	2	38	theme	electron	466:473	arg1	microscopy					475:484	scanning electron microscopy	457:484	scanning electron microscopy (SEM)	457:490	Then, properties of composite hydrogels with various OKGs were investigated via a series of methods, including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests.
33309658	2	39	theme	biocompatibility	518:533	arg1	spectroscopy					436:447	spectroscopy	436:447	spectroscopy (FTIR)	436:454	Then, properties of composite hydrogels with various OKGs were investigated via a series of methods, including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests.
33309658	2	39	theme	biocompatibility	518:533	arg1	tests					535:539	mechanical and biocompatibility tests	503:539	mechanical and biocompatibility tests	503:539	Then, properties of composite hydrogels with various OKGs were investigated via a series of methods, including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests.
33309658	6	40	theme	facile	1017:1022	arg1	method					1024:1029	a facile method	1015:1029	a facile method to promote the overall properties of gelatin-based hydrogels	1015:1090	In brief, this work provides a facile method to promote the overall properties of gelatin-based hydrogels, meanwhile revealed the relationship and mechanism underlying the effects of OKG with different Mw on composite hydrogels.
33309658	0	41	theme	konjac	48:53	arg1	effect					29:34	the tunable effect	17:34	the tunable effect of oxidized konjac	17:53	Investigation on the tunable effect of oxidized konjac glucomannan with different molecular weight on gelatin-based composite hydrogels.
33309658	4	42	theme	mechanical	712:721	arg1	strength					723:730	mechanical strength	712:730	mechanical strength	712:730	Besides, composite hydrogels not only showed increased mechanical strength, but self-healing ability at the same time, which were closely affected by the Mw of OKG.
33309658	0	43	theme	oxidized	39:46	arg1	konjac					48:53	oxidized konjac	39:53	oxidized konjac	39:53	Investigation on the tunable effect of oxidized konjac glucomannan with different molecular weight on gelatin-based composite hydrogels.
33309658	2	44	dep	transform	417:425	arg1	infrared					427:434	infrared	427:434	transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests	417:539	Then, properties of composite hydrogels with various OKGs were investigated via a series of methods, including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests.
33309658	2	45	theme	various	343:349	arg1	OKGs					351:354	various OKGs	343:354	various OKGs	343:354	Then, properties of composite hydrogels with various OKGs were investigated via a series of methods, including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests.
33309658	4	46	dep	showed	695:700	arg1	only					690:693	only	690:693	only	690:693	Besides, composite hydrogels not only showed increased mechanical strength, but self-healing ability at the same time, which were closely affected by the Mw of OKG.
33309658	4	46	dep	showed	695:700	arg1	increased					702:710	increased	702:710	increased	702:710	Besides, composite hydrogels not only showed increased mechanical strength, but self-healing ability at the same time, which were closely affected by the Mw of OKG.
33309658	2	47	theme	hydrogels	328:336	arg1	properties					304:313	properties	304:313	properties of composite hydrogels with various OKGs	304:354	Then, properties of composite hydrogels with various OKGs were investigated via a series of methods, including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests.
33309658	3	48	with	entanglement	604:615	arg1	Mw					653:654	higher Mw	646:654	higher Mw	646:654	The results confirmed an increased degree of crosslinking and entanglement between gelatin and OKG with higher Mw.
33309658	0	49	from	weight	92:97	arg1	hydrogels					126:134	gelatin-based composite hydrogels	102:134	gelatin-based composite hydrogels	102:134	Investigation on the tunable effect of oxidized konjac glucomannan with different molecular weight on gelatin-based composite hydrogels.
33309658	1	50	theme	oxidized	145:152	arg1	crosslinker					248:258	polysaccharide crosslinker	233:258	polysaccharide crosslinker	233:258	Herein, oxidized konjac glucomannan (OKG) with different molecular weight (Mw) were prepared as polysaccharide crosslinker to reinforce gelatin-based hydrogels.
33309658	1	50	theme	oxidized	145:152	arg1	OKG					174:176	OKG	174:176	OKG	174:176	Herein, oxidized konjac glucomannan (OKG) with different molecular weight (Mw) were prepared as polysaccharide crosslinker to reinforce gelatin-based hydrogels.
33309658	1	50	theme	oxidized	145:152	arg1	glucomannan					161:171	oxidized konjac glucomannan	145:171	oxidized konjac glucomannan (OKG) with different molecular weight (Mw)	145:214	Herein, oxidized konjac glucomannan (OKG) with different molecular weight (Mw) were prepared as polysaccharide crosslinker to reinforce gelatin-based hydrogels.
33309658	5	51	theme	composite	840:848	arg1	hydrogels					850:858	both composite hydrogels	835:858	both composite hydrogels	835:858	Furthermore, both composite hydrogels could support well proliferation of cells, which showed excellent capacity in tissue engineering and biomedical applications.
33309658	2	52	theme	scanning	457:464	arg1	spectroscopy					436:447	spectroscopy	436:447	spectroscopy (FTIR)	436:454	Then, properties of composite hydrogels with various OKGs were investigated via a series of methods, including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests.
33309658	2	52	theme	scanning	457:464	arg1	SEM					487:489	SEM	487:489	SEM	487:489	Then, properties of composite hydrogels with various OKGs were investigated via a series of methods, including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests.
33309658	2	52	theme	scanning	457:464	arg1	microscopy					475:484	scanning electron microscopy	457:484	scanning electron microscopy (SEM)	457:490	Then, properties of composite hydrogels with various OKGs were investigated via a series of methods, including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests.
33309658	1	53	theme	konjac	154:159	arg1	crosslinker					248:258	polysaccharide crosslinker	233:258	polysaccharide crosslinker	233:258	Herein, oxidized konjac glucomannan (OKG) with different molecular weight (Mw) were prepared as polysaccharide crosslinker to reinforce gelatin-based hydrogels.
33309658	1	53	theme	konjac	154:159	arg1	OKG					174:176	OKG	174:176	OKG	174:176	Herein, oxidized konjac glucomannan (OKG) with different molecular weight (Mw) were prepared as polysaccharide crosslinker to reinforce gelatin-based hydrogels.
33309658	1	53	theme	konjac	154:159	arg1	glucomannan					161:171	oxidized konjac glucomannan	145:171	oxidized konjac glucomannan (OKG) with different molecular weight (Mw)	145:214	Herein, oxidized konjac glucomannan (OKG) with different molecular weight (Mw) were prepared as polysaccharide crosslinker to reinforce gelatin-based hydrogels.
32933687	4	0	theme	usage	534:538	arg1	amount					524:529	its amount	520:529	its amount of usage	520:538	The utilization of silver was enhanced and its amount of usage could be obviously reduced by introduction of nontoxic chitosan which was benefical for the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep by the interaction between the silver ion and the plentiful amino and hydroxyl groups on the chitosan, thus improved the antibacterial activities and stability.
32933687	4	0	theme	usage	534:538	arg1	usage					534:538	usage	534:538	usage	534:538	The utilization of silver was enhanced and its amount of usage could be obviously reduced by introduction of nontoxic chitosan which was benefical for the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep by the interaction between the silver ion and the plentiful amino and hydroxyl groups on the chitosan, thus improved the antibacterial activities and stability.
32933687	1	1	theme	CTs	199:201	arg1	Ag/Sep					203:208	CTs@Ag/Sep	199:208	CTs@Ag/Sep	199:208	Organic-inorganic antibacterial materials chitosan@silver/sepiolite (CTs@Ag/Sep) was prepared by grafting organometallic chelate of chitosan and silver on sepiolite.
32933687	1	1	theme	CTs	199:201	arg1	silver/sepiolite					181:196	chitosan@silver/sepiolite	172:196	chitosan@silver/sepiolite (CTs@Ag/Sep)	172:209	Organic-inorganic antibacterial materials chitosan@silver/sepiolite (CTs@Ag/Sep) was prepared by grafting organometallic chelate of chitosan and silver on sepiolite.
32933687	5	2	dep	coli	972:975	arg1	coli					981:984	E. coli	978:984	E. coli	978:984	The average width of the inhibition zone of CTs@Ag/Sep against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Aspergillus niger were 51.8, 31.8, and 44.7 mm, respectively.
32933687	0	3	theme	antibacterial	92:104	arg1	activity					106:113	high synergistic antibacterial activity	75:113	high synergistic antibacterial activity	75:113	Preparation of organic-inorganic chitosan@silver/sepiolite composites with high synergistic antibacterial activity and stability.
32933687	5	4	theme	Ag/Sep	910:915	arg1	zone					898:901	the inhibition zone	883:901	the inhibition zone of CTs@Ag/Sep against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Aspergillus niger	883:1007	The average width of the inhibition zone of CTs@Ag/Sep against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Aspergillus niger were 51.8, 31.8, and 44.7 mm, respectively.
32933687	1	5	theme	@	202:202	arg1	Ag/Sep					203:208	CTs@Ag/Sep	199:208	CTs@Ag/Sep	199:208	Organic-inorganic antibacterial materials chitosan@silver/sepiolite (CTs@Ag/Sep) was prepared by grafting organometallic chelate of chitosan and silver on sepiolite.
32933687	1	5	theme	@	202:202	arg1	silver/sepiolite					181:196	chitosan@silver/sepiolite	172:196	chitosan@silver/sepiolite (CTs@Ag/Sep)	172:209	Organic-inorganic antibacterial materials chitosan@silver/sepiolite (CTs@Ag/Sep) was prepared by grafting organometallic chelate of chitosan and silver on sepiolite.
32933687	4	6	from	formation	632:640	arg1	Ag/Sep					694:699	the CTs@Ag/Sep	686:699	the CTs@Ag/Sep	686:699	The utilization of silver was enhanced and its amount of usage could be obviously reduced by introduction of nontoxic chitosan which was benefical for the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep by the interaction between the silver ion and the plentiful amino and hydroxyl groups on the chitosan, thus improved the antibacterial activities and stability.
32933687	4	7	theme	hydroxyl	771:778	arg1	groups					780:785	the plentiful amino and hydroxyl groups	747:785	groups	780:785	The utilization of silver was enhanced and its amount of usage could be obviously reduced by introduction of nontoxic chitosan which was benefical for the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep by the interaction between the silver ion and the plentiful amino and hydroxyl groups on the chitosan, thus improved the antibacterial activities and stability.
32933687	5	8	dep	S.	948:949	arg1	aureus					951:956	S. aureus	948:956	S. aureus	948:956	The average width of the inhibition zone of CTs@Ag/Sep against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Aspergillus niger were 51.8, 31.8, and 44.7 mm, respectively.
32933687	4	9	theme	silver	732:737	arg1	ion					739:741	the silver ion	728:741	the silver ion	728:741	The utilization of silver was enhanced and its amount of usage could be obviously reduced by introduction of nontoxic chitosan which was benefical for the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep by the interaction between the silver ion and the plentiful amino and hydroxyl groups on the chitosan, thus improved the antibacterial activities and stability.
32933687	6	10	theme	Ag/Sep	1077:1082	arg1	cytotoxicity					1057:1068	The cytotoxicity	1053:1068	The cytotoxicity of CTs@Ag/Sep	1053:1082	The cytotoxicity of CTs@Ag/Sep was further evaluated with A549 cells, and the results indicated that it exhibited low cytotoxicity to eukaryotic cells.
32933687	5	11	theme	average	866:872	arg1	width					874:878	The average width	862:878	The average width of the inhibition zone of CTs@Ag/Sep against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Aspergillus niger	862:1007	The average width of the inhibition zone of CTs@Ag/Sep against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Aspergillus niger were 51.8, 31.8, and 44.7 mm, respectively.
32933687	5	11	theme	average	866:872	arg1	mm					1035:1036	51.8, 31.8, and 44.7 mm	1014:1036	51.8, 31.8, and 44.7 mm	1014:1036	The average width of the inhibition zone of CTs@Ag/Sep against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Aspergillus niger were 51.8, 31.8, and 44.7 mm, respectively.
32933687	5	12	theme	zone	898:901	arg1	width					874:878	The average width	862:878	The average width of the inhibition zone of CTs@Ag/Sep against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Aspergillus niger	862:1007	The average width of the inhibition zone of CTs@Ag/Sep against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Aspergillus niger were 51.8, 31.8, and 44.7 mm, respectively.
32933687	5	12	theme	zone	898:901	arg1	mm					1035:1036	51.8, 31.8, and 44.7 mm	1014:1036	51.8, 31.8, and 44.7 mm	1014:1036	The average width of the inhibition zone of CTs@Ag/Sep against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Aspergillus niger were 51.8, 31.8, and 44.7 mm, respectively.
32933687	3	13	theme	carrier	391:397	arg1	beneficial					413:422	beneficial	413:422	beneficial	413:422	The carrier sepiolite was beneficial for the thermal stability of the composite material.
32933687	3	13	theme	carrier	391:397	arg1	sepiolite					399:407	The carrier sepiolite	387:407	The carrier sepiolite	387:407	The carrier sepiolite was beneficial for the thermal stability of the composite material.
32933687	6	14	theme	CTs	1073:1075	arg1	Ag/Sep					1077:1082	CTs@Ag/Sep	1073:1082	CTs@Ag/Sep	1073:1082	The cytotoxicity of CTs@Ag/Sep was further evaluated with A549 cells, and the results indicated that it exhibited low cytotoxicity to eukaryotic cells.
32933687	4	15	from	groups	780:785	arg1	chitosan					794:801	the chitosan	790:801	the chitosan	790:801	The utilization of silver was enhanced and its amount of usage could be obviously reduced by introduction of nontoxic chitosan which was benefical for the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep by the interaction between the silver ion and the plentiful amino and hydroxyl groups on the chitosan, thus improved the antibacterial activities and stability.
32933687	2	16	theme	prepared	300:307	arg1	samples					309:315	The prepared samples	296:315	The prepared samples	296:315	The prepared samples were characterized, and their antibacterial properties were detected.
32933687	4	17	theme	@	693:693	arg1	Ag/Sep					694:699	the CTs@Ag/Sep	686:699	the CTs@Ag/Sep	686:699	The utilization of silver was enhanced and its amount of usage could be obviously reduced by introduction of nontoxic chitosan which was benefical for the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep by the interaction between the silver ion and the plentiful amino and hydroxyl groups on the chitosan, thus improved the antibacterial activities and stability.
32933687	2	18	theme	antibacterial	347:359	arg1	properties					361:370	their antibacterial properties	341:370	their antibacterial properties	341:370	The prepared samples were characterized, and their antibacterial properties were detected.
32933687	3	19	theme	material	467:474	arg1	stability					440:448	the thermal stability	428:448	the thermal stability of the composite material	428:474	The carrier sepiolite was beneficial for the thermal stability of the composite material.
32933687	3	20	theme	thermal	432:438	arg1	stability					440:448	the thermal stability	428:448	the thermal stability of the composite material	428:474	The carrier sepiolite was beneficial for the thermal stability of the composite material.
32933687	4	21	theme	chitosan	595:602	arg1	introduction					570:581	introduction	570:581	introduction of nontoxic chitosan which was benefical for the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep	570:699	The utilization of silver was enhanced and its amount of usage could be obviously reduced by introduction of nontoxic chitosan which was benefical for the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep by the interaction between the silver ion and the plentiful amino and hydroxyl groups on the chitosan, thus improved the antibacterial activities and stability.
32933687	0	22	theme	chitosan	33:40	arg1	composites					59:68	organic-inorganic chitosan@silver/sepiolite composites	15:68	organic-inorganic chitosan@silver/sepiolite composites	15:68	Preparation of organic-inorganic chitosan@silver/sepiolite composites with high synergistic antibacterial activity and stability.
32933687	4	23	theme	nontoxic	586:593	arg1	chitosan					595:602	nontoxic chitosan	586:602	nontoxic chitosan which was benefical for the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep	586:699	The utilization of silver was enhanced and its amount of usage could be obviously reduced by introduction of nontoxic chitosan which was benefical for the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep by the interaction between the silver ion and the plentiful amino and hydroxyl groups on the chitosan, thus improved the antibacterial activities and stability.
32933687	4	24	from	amino	761:765	arg1	chitosan					794:801	the chitosan	790:801	the chitosan	790:801	The utilization of silver was enhanced and its amount of usage could be obviously reduced by introduction of nontoxic chitosan which was benefical for the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep by the interaction between the silver ion and the plentiful amino and hydroxyl groups on the chitosan, thus improved the antibacterial activities and stability.
32933687	6	25	theme	A549	1111:1114	arg1	cells					1116:1120	A549 cells	1111:1120	A549 cells	1111:1120	The cytotoxicity of CTs@Ag/Sep was further evaluated with A549 cells, and the results indicated that it exhibited low cytotoxicity to eukaryotic cells.
32933687	0	26	theme	organic-inorganic	15:31	arg1	composites					59:68	organic-inorganic chitosan@silver/sepiolite composites	15:68	organic-inorganic chitosan@silver/sepiolite composites	15:68	Preparation of organic-inorganic chitosan@silver/sepiolite composites with high synergistic antibacterial activity and stability.
32933687	4	27	theme	silver	496:501	arg1	utilization					481:491	The utilization	477:491	The utilization of silver	477:501	The utilization of silver was enhanced and its amount of usage could be obviously reduced by introduction of nontoxic chitosan which was benefical for the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep by the interaction between the silver ion and the plentiful amino and hydroxyl groups on the chitosan, thus improved the antibacterial activities and stability.
32933687	1	28	theme	Organic-inorganic	130:146	arg1	materials					162:170	Organic-inorganic antibacterial materials	130:170	Organic-inorganic antibacterial materials chitosan@silver/sepiolite (CTs@Ag/Sep) was prepared by grafting organometallic chelate of chitosan and silver on sepiolite.	130:294	Organic-inorganic antibacterial materials chitosan@silver/sepiolite (CTs@Ag/Sep) was prepared by grafting organometallic chelate of chitosan and silver on sepiolite.
32933687	4	29	theme	nanoparticles	669:681	arg1	formation					632:640	the formation	628:640	the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep	628:699	The utilization of silver was enhanced and its amount of usage could be obviously reduced by introduction of nontoxic chitosan which was benefical for the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep by the interaction between the silver ion and the plentiful amino and hydroxyl groups on the chitosan, thus improved the antibacterial activities and stability.
32933687	0	30	theme	silver/sepiolite	42:57	arg1	composites					59:68	organic-inorganic chitosan@silver/sepiolite composites	15:68	organic-inorganic chitosan@silver/sepiolite composites	15:68	Preparation of organic-inorganic chitosan@silver/sepiolite composites with high synergistic antibacterial activity and stability.
32933687	6	31	theme	@	1076:1076	arg1	Ag/Sep					1077:1082	CTs@Ag/Sep	1073:1082	CTs@Ag/Sep	1073:1082	The cytotoxicity of CTs@Ag/Sep was further evaluated with A549 cells, and the results indicated that it exhibited low cytotoxicity to eukaryotic cells.
32933687	4	32	theme	CTs	690:692	arg1	Ag/Sep					694:699	the CTs@Ag/Sep	686:699	the CTs@Ag/Sep	686:699	The utilization of silver was enhanced and its amount of usage could be obviously reduced by introduction of nontoxic chitosan which was benefical for the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep by the interaction between the silver ion and the plentiful amino and hydroxyl groups on the chitosan, thus improved the antibacterial activities and stability.
32933687	1	33	theme	organometallic	236:249	arg1	chelate					251:257	organometallic chelate	236:257	organometallic chelate of chitosan and silver on sepiolite	236:293	Organic-inorganic antibacterial materials chitosan@silver/sepiolite (CTs@Ag/Sep) was prepared by grafting organometallic chelate of chitosan and silver on sepiolite.
32933687	0	34	theme	@	41:41	arg1	composites					59:68	organic-inorganic chitosan@silver/sepiolite composites	15:68	organic-inorganic chitosan@silver/sepiolite composites	15:68	Preparation of organic-inorganic chitosan@silver/sepiolite composites with high synergistic antibacterial activity and stability.
32933687	3	35	theme	composite	457:465	arg1	material					467:474	the composite material	453:474	the composite material	453:474	The carrier sepiolite was beneficial for the thermal stability of the composite material.
32933687	6	36	theme	eukaryotic	1187:1196	arg1	cells					1198:1202	eukaryotic cells	1187:1202	eukaryotic cells	1187:1202	The cytotoxicity of CTs@Ag/Sep was further evaluated with A549 cells, and the results indicated that it exhibited low cytotoxicity to eukaryotic cells.
32933687	1	37	from	chelate	251:257	arg1	sepiolite					285:293	sepiolite	285:293	sepiolite	285:293	Organic-inorganic antibacterial materials chitosan@silver/sepiolite (CTs@Ag/Sep) was prepared by grafting organometallic chelate of chitosan and silver on sepiolite.
32933687	1	38	theme	antibacterial	148:160	arg1	materials					162:170	Organic-inorganic antibacterial materials	130:170	Organic-inorganic antibacterial materials chitosan@silver/sepiolite (CTs@Ag/Sep) was prepared by grafting organometallic chelate of chitosan and silver on sepiolite.	130:294	Organic-inorganic antibacterial materials chitosan@silver/sepiolite (CTs@Ag/Sep) was prepared by grafting organometallic chelate of chitosan and silver on sepiolite.
32933687	0	39	theme	composites	59:68	arg1	Preparation					0:10	Preparation	0:10	Preparation of organic-inorganic chitosan@silver/sepiolite composites with high synergistic antibacterial activity and stability.	0:128	Preparation of organic-inorganic chitosan@silver/sepiolite composites with high synergistic antibacterial activity and stability.
32933687	4	40	theme	plentiful	751:759	arg1	amino					761:765	the plentiful amino and hydroxyl groups	747:785	amino	761:765	The utilization of silver was enhanced and its amount of usage could be obviously reduced by introduction of nontoxic chitosan which was benefical for the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep by the interaction between the silver ion and the plentiful amino and hydroxyl groups on the chitosan, thus improved the antibacterial activities and stability.
32933687	6	41	theme	low	1167:1169	arg1	cytotoxicity					1171:1182	low cytotoxicity	1167:1182	low cytotoxicity to eukaryotic cells	1167:1202	The cytotoxicity of CTs@Ag/Sep was further evaluated with A549 cells, and the results indicated that it exhibited low cytotoxicity to eukaryotic cells.
32933687	0	42	with	Preparation	0:10	arg1	stability					119:127	stability	119:127	stability	119:127	Preparation of organic-inorganic chitosan@silver/sepiolite composites with high synergistic antibacterial activity and stability.
32933687	0	42	with	Preparation	0:10	arg1	activity					106:113	high synergistic antibacterial activity	75:113	high synergistic antibacterial activity	75:113	Preparation of organic-inorganic chitosan@silver/sepiolite composites with high synergistic antibacterial activity and stability.
32933687	1	43	theme	chitosan	172:179	arg1	Ag/Sep					203:208	CTs@Ag/Sep	199:208	CTs@Ag/Sep	199:208	Organic-inorganic antibacterial materials chitosan@silver/sepiolite (CTs@Ag/Sep) was prepared by grafting organometallic chelate of chitosan and silver on sepiolite.
32933687	1	43	theme	chitosan	172:179	arg1	silver/sepiolite					181:196	chitosan@silver/sepiolite	172:196	chitosan@silver/sepiolite (CTs@Ag/Sep)	172:209	Organic-inorganic antibacterial materials chitosan@silver/sepiolite (CTs@Ag/Sep) was prepared by grafting organometallic chelate of chitosan and silver on sepiolite.
32933687	1	44	dep	materials	162:170	arg1	prepared					215:222	prepared	215:222	was prepared by grafting organometallic chelate of chitosan and silver on sepiolite	211:293	Organic-inorganic antibacterial materials chitosan@silver/sepiolite (CTs@Ag/Sep) was prepared by grafting organometallic chelate of chitosan and silver on sepiolite.
32933687	1	45	theme	@	180:180	arg1	Ag/Sep					203:208	CTs@Ag/Sep	199:208	CTs@Ag/Sep	199:208	Organic-inorganic antibacterial materials chitosan@silver/sepiolite (CTs@Ag/Sep) was prepared by grafting organometallic chelate of chitosan and silver on sepiolite.
32933687	1	45	theme	@	180:180	arg1	silver/sepiolite					181:196	chitosan@silver/sepiolite	172:196	chitosan@silver/sepiolite (CTs@Ag/Sep)	172:209	Organic-inorganic antibacterial materials chitosan@silver/sepiolite (CTs@Ag/Sep) was prepared by grafting organometallic chelate of chitosan and silver on sepiolite.
32933687	1	46	theme	chitosan	262:269	arg1	chelate					251:257	organometallic chelate	236:257	organometallic chelate of chitosan and silver on sepiolite	236:293	Organic-inorganic antibacterial materials chitosan@silver/sepiolite (CTs@Ag/Sep) was prepared by grafting organometallic chelate of chitosan and silver on sepiolite.
32933687	0	47	theme	synergistic	80:90	arg1	activity					106:113	high synergistic antibacterial activity	75:113	high synergistic antibacterial activity	75:113	Preparation of organic-inorganic chitosan@silver/sepiolite composites with high synergistic antibacterial activity and stability.
32933687	4	48	from	interaction	708:718	arg1	chitosan					794:801	the chitosan	790:801	the chitosan	790:801	The utilization of silver was enhanced and its amount of usage could be obviously reduced by introduction of nontoxic chitosan which was benefical for the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep by the interaction between the silver ion and the plentiful amino and hydroxyl groups on the chitosan, thus improved the antibacterial activities and stability.
32933687	4	49	theme	antibacterial	822:834	arg1	activities					836:845	the antibacterial activities	818:845	the antibacterial activities	818:845	The utilization of silver was enhanced and its amount of usage could be obviously reduced by introduction of nontoxic chitosan which was benefical for the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep by the interaction between the silver ion and the plentiful amino and hydroxyl groups on the chitosan, thus improved the antibacterial activities and stability.
32933687	5	50	theme	CTs	906:908	arg1	Ag/Sep					910:915	CTs@Ag/Sep	906:915	CTs@Ag/Sep against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Aspergillus niger	906:1007	The average width of the inhibition zone of CTs@Ag/Sep against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Aspergillus niger were 51.8, 31.8, and 44.7 mm, respectively.
32933687	5	51	theme	inhibition	887:896	arg1	zone					898:901	the inhibition zone	883:901	the inhibition zone of CTs@Ag/Sep against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Aspergillus niger	883:1007	The average width of the inhibition zone of CTs@Ag/Sep against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Aspergillus niger were 51.8, 31.8, and 44.7 mm, respectively.
32933687	4	52	theme	silver	662:667	arg1	nanoparticles					669:681	highly dispersed silver nanoparticles	645:681	highly dispersed silver nanoparticles	645:681	The utilization of silver was enhanced and its amount of usage could be obviously reduced by introduction of nontoxic chitosan which was benefical for the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep by the interaction between the silver ion and the plentiful amino and hydroxyl groups on the chitosan, thus improved the antibacterial activities and stability.
32933687	0	53	theme	high	75:78	arg1	activity					106:113	high synergistic antibacterial activity	75:113	high synergistic antibacterial activity	75:113	Preparation of organic-inorganic chitosan@silver/sepiolite composites with high synergistic antibacterial activity and stability.
32933687	4	54	theme	dispersed	652:660	arg1	nanoparticles					669:681	highly dispersed silver nanoparticles	645:681	highly dispersed silver nanoparticles	645:681	The utilization of silver was enhanced and its amount of usage could be obviously reduced by introduction of nontoxic chitosan which was benefical for the formation of highly dispersed silver nanoparticles on the CTs@Ag/Sep by the interaction between the silver ion and the plentiful amino and hydroxyl groups on the chitosan, thus improved the antibacterial activities and stability.
32933687	5	55	theme	@	909:909	arg1	Ag/Sep					910:915	CTs@Ag/Sep	906:915	CTs@Ag/Sep against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Aspergillus niger	906:1007	The average width of the inhibition zone of CTs@Ag/Sep against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Aspergillus niger were 51.8, 31.8, and 44.7 mm, respectively.
32933687	1	56	theme	silver	275:280	arg1	chelate					251:257	organometallic chelate	236:257	organometallic chelate of chitosan and silver on sepiolite	236:293	Organic-inorganic antibacterial materials chitosan@silver/sepiolite (CTs@Ag/Sep) was prepared by grafting organometallic chelate of chitosan and silver on sepiolite.
33518056	8	0	theme	serum	1229:1233	arg1	levels					1235:1240	serum levels	1229:1240	serum levels of urea	1229:1248	BAC-CAR modified serum levels of urea more efficient than CAR (P < 0.05).
33518056	4	1	theme	foods	603:607	arg1	composition					588:598	chemical composition	579:598	chemical composition of foods	579:607	Since chemical composition of foods can alter toxicity of pesticides, in this work, the effect of coadministration of CAR and organophosphates on renal function has been studied and compared with the effect of coadministration of carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers.
33518056	7	2	theme	renal	1192:1196	arg1	inflammation					1198:1209	renal inflammation	1192:1209	renal inflammation	1192:1209	However, coadministration of CAR or BAC-CAR modified urea and creatinine and improved renal inflammation.
33518056	4	3	theme	renal	719:723	arg1	function					725:732	renal function	719:732	renal function	719:732	Since chemical composition of foods can alter toxicity of pesticides, in this work, the effect of coadministration of CAR and organophosphates on renal function has been studied and compared with the effect of coadministration of carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers.
33518056	7	4	theme	BAC-CAR	1142:1148	arg1	coadministration					1115:1130	coadministration	1115:1130	coadministration of CAR or BAC-CAR	1115:1148	However, coadministration of CAR or BAC-CAR modified urea and creatinine and improved renal inflammation.
33518056	10	5	theme	pesticides	1445:1454	arg1	formulation					1414:1424	the formulation	1410:1424	the formulation of organophosphate pesticides, which may control pests more efficiently than conventional organophosphate pesticides	1410:1541	Carvacrol can be used in the formulation of organophosphate pesticides, which may control pests more efficiently than conventional organophosphate pesticides.
33518056	3	6	theme	main	532:535	arg1	component					537:545	the main component	528:545	the main component of Satureja khuzestanica	528:570	Carvacrol (CAR) is the main component of Satureja khuzestanica.
33518056	3	6	theme	main	532:535	arg1	Carvacrol					509:517	Carvacrol	509:517	Carvacrol (CAR)	509:523	Carvacrol (CAR) is the main component of Satureja khuzestanica.
33518056	4	7	theme	based	862:866	arg1	nanoflowers					868:878	carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers	803:878	carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers	803:878	Since chemical composition of foods can alter toxicity of pesticides, in this work, the effect of coadministration of CAR and organophosphates on renal function has been studied and compared with the effect of coadministration of carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers.
33518056	2	8	used	used	411:414	arg2	organophosphates					359:374	organophosphates	359:374	organophosphates such as malathion	359:392	Among insecticides organophosphates such as malathion have been widely used around the world and others such as parathion has been restricted because of their toxicity.
33518056	2	8	used	used	411:414	arg2	malathion					384:392	malathion	384:392	malathion	384:392	Among insecticides organophosphates such as malathion have been widely used around the world and others such as parathion has been restricted because of their toxicity.
33518056	5	9	theme	examination	934:944	arg1	levels					887:892	Serum levels	881:892	Serum levels of urea and creatinine and histological examination	881:944	Serum levels of urea and creatinine and histological examination were analyzed after 10 days of administration of chemicals.
33518056	3	10	theme	Satureja	550:557	arg1	khuzestanica					559:570	Satureja khuzestanica	550:570	Satureja khuzestanica	550:570	Carvacrol (CAR) is the main component of Satureja khuzestanica.
33518056	4	11	theme	coadministration	671:686	arg1	effect					661:666	the effect	657:666	the effect of coadministration of CAR and organophosphates on renal function	657:732	Since chemical composition of foods can alter toxicity of pesticides, in this work, the effect of coadministration of CAR and organophosphates on renal function has been studied and compared with the effect of coadministration of carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers.
33518056	8	12	theme	urea	1245:1248	arg1	levels					1235:1240	serum levels	1229:1240	serum levels of urea	1229:1248	BAC-CAR modified serum levels of urea more efficient than CAR (P < 0.05).
33518056	4	13	theme	nanoflowers	868:878	arg1	coadministration					783:798	coadministration	783:798	coadministration of carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers	783:878	Since chemical composition of foods can alter toxicity of pesticides, in this work, the effect of coadministration of CAR and organophosphates on renal function has been studied and compared with the effect of coadministration of carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers.
33518056	4	14	theme	coadministration	783:798	arg1	effect					773:778	the effect	769:778	the effect of coadministration of carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers	769:878	Since chemical composition of foods can alter toxicity of pesticides, in this work, the effect of coadministration of CAR and organophosphates on renal function has been studied and compared with the effect of coadministration of carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers.
33518056	5	15	theme	urea	897:900	arg1	levels					887:892	Serum levels	881:892	Serum levels of urea and creatinine and histological examination	881:944	Serum levels of urea and creatinine and histological examination were analyzed after 10 days of administration of chemicals.
33518056	9	16	theme	renal	1368:1372	arg1	disorders					1374:1382	renal disorders	1368:1382	renal disorders	1368:1382	It is concluded that BAC could be considered as a carrier for drugs used to treat renal disorders.
33518056	10	17	theme	organophosphate	1516:1530	arg1	pesticides					1532:1541	conventional organophosphate pesticides	1503:1541	conventional organophosphate pesticides	1503:1541	Carvacrol can be used in the formulation of organophosphate pesticides, which may control pests more efficiently than conventional organophosphate pesticides.
33518056	5	18	theme	administration	977:990	arg1	10 days					966:972	10 days	966:972	10 days of administration of chemicals	966:1003	Serum levels of urea and creatinine and histological examination were analyzed after 10 days of administration of chemicals.
33518056	4	19	theme	CAR	691:693	arg1	coadministration					671:686	coadministration	671:686	coadministration of CAR and organophosphates	671:714	Since chemical composition of foods can alter toxicity of pesticides, in this work, the effect of coadministration of CAR and organophosphates on renal function has been studied and compared with the effect of coadministration of carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers.
33518056	10	20	theme	conventional	1503:1514	arg1	pesticides					1532:1541	conventional organophosphate pesticides	1503:1541	conventional organophosphate pesticides	1503:1541	Carvacrol can be used in the formulation of organophosphate pesticides, which may control pests more efficiently than conventional organophosphate pesticides.
33518056	1	21	dep	billion	183:189	arg1	1.8					179:181	1.8	179:181	1.8	179:181	Most of approximately 1.8 billion people involved in agriculture protect their food products using pesticides especially insecticides which may remain in foods as pesticide residues.
33518056	4	22	from	effect	661:666	arg1	function					725:732	renal function	719:732	renal function	719:732	Since chemical composition of foods can alter toxicity of pesticides, in this work, the effect of coadministration of CAR and organophosphates on renal function has been studied and compared with the effect of coadministration of carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers.
33518056	5	23	theme	creatinine	906:915	arg1	levels					887:892	Serum levels	881:892	Serum levels of urea and creatinine and histological examination	881:944	Serum levels of urea and creatinine and histological examination were analyzed after 10 days of administration of chemicals.
33518056	0	24	theme	cyclodextrin-alginate-chitosan	22:51	arg1	nanoflowers					59:69	beta cyclodextrin-alginate-chitosan based nanoflowers	17:69	beta cyclodextrin-alginate-chitosan based nanoflowers	17:69	Carvacrol loaded beta cyclodextrin-alginate-chitosan based nanoflowers attenuates renal toxicity induced by malathion and parathion: A comparative toxicity.
33518056	4	25	theme	chemical	579:586	arg1	composition					588:598	chemical composition	579:598	chemical composition of foods	579:607	Since chemical composition of foods can alter toxicity of pesticides, in this work, the effect of coadministration of CAR and organophosphates on renal function has been studied and compared with the effect of coadministration of carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers.
33518056	5	26	theme	chemicals	995:1003	arg1	administration					977:990	administration	977:990	administration of chemicals	977:1003	Serum levels of urea and creatinine and histological examination were analyzed after 10 days of administration of chemicals.
33518056	0	27	theme	beta	17:20	arg1	nanoflowers					59:69	beta cyclodextrin-alginate-chitosan based nanoflowers	17:69	beta cyclodextrin-alginate-chitosan based nanoflowers	17:69	Carvacrol loaded beta cyclodextrin-alginate-chitosan based nanoflowers attenuates renal toxicity induced by malathion and parathion: A comparative toxicity.
33518056	1	28	theme	pesticide	320:328	arg1	residues					330:337	pesticide residues	320:337	pesticide residues	320:337	Most of approximately 1.8 billion people involved in agriculture protect their food products using pesticides especially insecticides which may remain in foods as pesticide residues.
33518056	0	29	theme	comparative	135:145	arg1	toxicity					147:154	A comparative toxicity	133:154	A comparative toxicity	133:154	Carvacrol loaded beta cyclodextrin-alginate-chitosan based nanoflowers attenuates renal toxicity induced by malathion and parathion: A comparative toxicity.
33518056	5	30	theme	Serum	881:885	arg1	levels					887:892	Serum levels	881:892	Serum levels of urea and creatinine and histological examination	881:944	Serum levels of urea and creatinine and histological examination were analyzed after 10 days of administration of chemicals.
33518056	1	31	theme	food	236:239	arg1	products					241:248	their food products	230:248	their food products using pesticides	230:265	Most of approximately 1.8 billion people involved in agriculture protect their food products using pesticides especially insecticides which may remain in foods as pesticide residues.
33518056	0	32	theme	based	53:57	arg1	nanoflowers					59:69	beta cyclodextrin-alginate-chitosan based nanoflowers	17:69	beta cyclodextrin-alginate-chitosan based nanoflowers	17:69	Carvacrol loaded beta cyclodextrin-alginate-chitosan based nanoflowers attenuates renal toxicity induced by malathion and parathion: A comparative toxicity.
33518056	7	33	theme	CAR	1135:1137	arg1	coadministration					1115:1130	coadministration	1115:1130	coadministration of CAR or BAC-CAR	1115:1148	However, coadministration of CAR or BAC-CAR modified urea and creatinine and improved renal inflammation.
33518056	0	34	theme	renal	82:86	arg1	toxicity					88:95	renal toxicity	82:95	renal toxicity induced by malathion and parathion	82:130	Carvacrol loaded beta cyclodextrin-alginate-chitosan based nanoflowers attenuates renal toxicity induced by malathion and parathion: A comparative toxicity.
33518056	1	35	dep	people	191:196	arg1	billion					183:189	billion	183:189	billion	183:189	Most of approximately 1.8 billion people involved in agriculture protect their food products using pesticides especially insecticides which may remain in foods as pesticide residues.
33518056	10	36	theme	organophosphate	1429:1443	arg1	pesticides					1445:1454	organophosphate pesticides	1429:1454	organophosphate pesticides	1429:1454	Carvacrol can be used in the formulation of organophosphate pesticides, which may control pests more efficiently than conventional organophosphate pesticides.
33518056	4	37	theme	loaded	813:818	arg1	nanoflowers					868:878	carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers	803:878	carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers	803:878	Since chemical composition of foods can alter toxicity of pesticides, in this work, the effect of coadministration of CAR and organophosphates on renal function has been studied and compared with the effect of coadministration of carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers.
33518056	6	38	theme	renal	1086:1090	arg1	inflammation					1092:1103	renal inflammation	1086:1103	renal inflammation	1086:1103	Malathion and parathion significantly increased urea and creatinine and induced renal inflammation.
33518056	10	39	used	used	1402:1405	arg2	Carvacrol					1385:1393	Carvacrol	1385:1393	Carvacrol	1385:1393	Carvacrol can be used in the formulation of organophosphate pesticides, which may control pests more efficiently than conventional organophosphate pesticides.
33518056	3	40	theme	khuzestanica	559:570	arg1	component					537:545	the main component	528:545	the main component of Satureja khuzestanica	528:570	Carvacrol (CAR) is the main component of Satureja khuzestanica.
33518056	3	40	theme	khuzestanica	559:570	arg1	Carvacrol					509:517	Carvacrol	509:517	Carvacrol (CAR)	509:523	Carvacrol (CAR) is the main component of Satureja khuzestanica.
33518056	4	41	theme	carvacrol	803:811	arg1	nanoflowers					868:878	carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers	803:878	carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers	803:878	Since chemical composition of foods can alter toxicity of pesticides, in this work, the effect of coadministration of CAR and organophosphates on renal function has been studied and compared with the effect of coadministration of carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers.
33518056	4	42	theme	pesticides	631:640	arg1	toxicity					619:626	toxicity	619:626	toxicity of pesticides	619:640	Since chemical composition of foods can alter toxicity of pesticides, in this work, the effect of coadministration of CAR and organophosphates on renal function has been studied and compared with the effect of coadministration of carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers.
33518056	0	43	dep	loaded	10:15	arg1	attenuates					71:80	attenuates	71:80	loaded beta cyclodextrin-alginate-chitosan based nanoflowers attenuates renal toxicity induced by malathion and parathion	10:130	Carvacrol loaded beta cyclodextrin-alginate-chitosan based nanoflowers attenuates renal toxicity induced by malathion and parathion: A comparative toxicity.
33518056	0	43	dep	loaded	10:15	arg1	toxicity					147:154	A comparative toxicity	133:154	A comparative toxicity	133:154	Carvacrol loaded beta cyclodextrin-alginate-chitosan based nanoflowers attenuates renal toxicity induced by malathion and parathion: A comparative toxicity.
33518056	4	44	theme	organophosphates	699:714	arg1	coadministration					671:686	coadministration	671:686	coadministration of CAR and organophosphates	671:714	Since chemical composition of foods can alter toxicity of pesticides, in this work, the effect of coadministration of CAR and organophosphates on renal function has been studied and compared with the effect of coadministration of carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers.
33518056	4	45	theme	beta	820:823	arg1	nanoflowers					868:878	carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers	803:878	carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers	803:878	Since chemical composition of foods can alter toxicity of pesticides, in this work, the effect of coadministration of CAR and organophosphates on renal function has been studied and compared with the effect of coadministration of carvacrol loaded beta cyclodextrin-alginate-chitosan (BAC) based nanoflowers.
33518056	5	46	theme	histological	921:932	arg1	examination					934:944	histological examination	921:944	histological examination	921:944	Serum levels of urea and creatinine and histological examination were analyzed after 10 days of administration of chemicals.
33947555	5	0	theme	excessive	910:918	arg1	blood					920:924	excessive blood	910:924	excessive blood	910:924	Its porous and high surface area structure make it a potential candidate for wound dressing applications due to its ability to absorb excessive blood and exudates, as well as provide protection from infection while maintaining good thermal stability.
33947555	5	1	theme	high	791:794	arg1	area					804:807	high surface area	791:807	high surface area	791:807	Its porous and high surface area structure make it a potential candidate for wound dressing applications due to its ability to absorb excessive blood and exudates, as well as provide protection from infection while maintaining good thermal stability.
33947555	2	2	from	loadings	324:331	arg1	addition					266:273	The addition	262:273	The addition of Aloe vera extract at different weight percent loadings	262:331	The addition of Aloe vera extract at different weight percent loadings was investigated.
33947555	2	3	theme	extract	288:294	arg1	addition					266:273	The addition	262:273	The addition of Aloe vera extract at different weight percent loadings	262:331	The addition of Aloe vera extract at different weight percent loadings was investigated.
33947555	5	4	from	infection	975:983	arg1	protection					959:968	protection	959:968	protection from infection	959:983	Its porous and high surface area structure make it a potential candidate for wound dressing applications due to its ability to absorb excessive blood and exudates, as well as provide protection from infection while maintaining good thermal stability.
33947555	2	5	theme	percent	316:322	arg1	loadings					324:331	different weight percent loadings	299:331	different weight percent loadings	299:331	The addition of Aloe vera extract at different weight percent loadings was investigated.
33947555	2	6	theme	vera	283:286	arg1	extract					288:294	Aloe vera extract	278:294	Aloe vera extract	278:294	The addition of Aloe vera extract at different weight percent loadings was investigated.
33947555	5	7	theme	wound	853:857	arg1	applications					868:879	wound dressing applications	853:879	wound dressing applications due to its ability to absorb excessive blood and exudates, as well as provide protection from infection while maintaining good thermal stability	853:1024	Its porous and high surface area structure make it a potential candidate for wound dressing applications due to its ability to absorb excessive blood and exudates, as well as provide protection from infection while maintaining good thermal stability.
33947555	3	8	theme	electron	481:488	arg1	SEM					502:504	SEM	502:504	SEM	502:504	The morphology, thermal properties, physical properties, and water absorption of the nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).
33947555	3	8	theme	electron	481:488	arg1	microscopy					490:499	scanning electron microscopy	472:499	scanning electron microscopy (SEM)	472:505	The morphology, thermal properties, physical properties, and water absorption of the nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).
33947555	1	9	theme	Natural	78:84	arg1	nanofibers					130:139	Natural, biocompatible, and biodegradable composite nanofibers	78:139	Natural, biocompatible, and biodegradable composite nanofibers made of Aloe vera extract, pullulan, chitosan, and citric acid	78:202	Natural, biocompatible, and biodegradable composite nanofibers made of Aloe vera extract, pullulan, chitosan, and citric acid were successfully produced via Forcespinning® technology.
33947555	3	10	theme	nanofibers	436:445	arg1	morphology					355:364	The morphology	351:364	The morphology	351:364	The morphology, thermal properties, physical properties, and water absorption of the nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).
33947555	3	10	theme	nanofibers	436:445	arg1	properties					375:384	thermal properties	367:384	thermal properties	367:384	The morphology, thermal properties, physical properties, and water absorption of the nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).
33947555	3	10	theme	nanofibers	436:445	arg1	properties					396:405	physical properties	387:405	physical properties	387:405	The morphology, thermal properties, physical properties, and water absorption of the nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).
33947555	3	10	theme	nanofibers	436:445	arg1	absorption					418:427	water absorption	412:427	water absorption	412:427	The morphology, thermal properties, physical properties, and water absorption of the nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).
33947555	5	11	theme	surface	796:802	arg1	area					804:807	high surface area	791:807	high surface area	791:807	Its porous and high surface area structure make it a potential candidate for wound dressing applications due to its ability to absorb excessive blood and exudates, as well as provide protection from infection while maintaining good thermal stability.
33947555	3	12	theme	thermal	367:373	arg1	properties					375:384	thermal properties	367:384	thermal properties	367:384	The morphology, thermal properties, physical properties, and water absorption of the nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).
33947555	3	13	theme	physical	387:394	arg1	properties					396:405	physical properties	387:405	physical properties	387:405	The morphology, thermal properties, physical properties, and water absorption of the nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).
33947555	1	14	theme	biocompatible	87:99	arg1	nanofibers					130:139	Natural, biocompatible, and biodegradable composite nanofibers	78:139	Natural, biocompatible, and biodegradable composite nanofibers made of Aloe vera extract, pullulan, chitosan, and citric acid	78:202	Natural, biocompatible, and biodegradable composite nanofibers made of Aloe vera extract, pullulan, chitosan, and citric acid were successfully produced via Forcespinning® technology.
33947555	4	15	theme	antibacterial	686:698	arg1	activity					700:707	synergistic antibacterial activity	674:707	synergistic antibacterial activity against Escherichia coli	674:732	The developed nanofiber membranes exhibited good water absorption capabilities, synergistic antibacterial activity against Escherichia coli, and promoted cell attachment and growth.
33947555	4	15	theme	antibacterial	686:698	arg1	capabilities					660:671	good water absorption capabilities	638:671	good water absorption capabilities	638:671	The developed nanofiber membranes exhibited good water absorption capabilities, synergistic antibacterial activity against Escherichia coli, and promoted cell attachment and growth.
33947555	0	16	theme	Aloe	0:3	arg1	Vera					5:8	Aloe Vera	0:8	Aloe Vera	0:8	Aloe Vera extract-based composite nanofibers for wound dressing applications.
33947555	2	17	theme	weight	309:314	arg1	loadings					324:331	different weight percent loadings	299:331	different weight percent loadings	299:331	The addition of Aloe vera extract at different weight percent loadings was investigated.
33947555	4	18	theme	synergistic	674:684	arg1	activity					700:707	synergistic antibacterial activity	674:707	synergistic antibacterial activity against Escherichia coli	674:732	The developed nanofiber membranes exhibited good water absorption capabilities, synergistic antibacterial activity against Escherichia coli, and promoted cell attachment and growth.
33947555	4	18	theme	synergistic	674:684	arg1	capabilities					660:671	good water absorption capabilities	638:671	good water absorption capabilities	638:671	The developed nanofiber membranes exhibited good water absorption capabilities, synergistic antibacterial activity against Escherichia coli, and promoted cell attachment and growth.
33947555	0	19	theme	composite	24:32	arg1	nanofibers					34:43	composite nanofibers	24:43	composite nanofibers for wound dressing applications	24:75	Aloe Vera extract-based composite nanofibers for wound dressing applications.
33947555	2	20	theme	different	299:307	arg1	loadings					324:331	different weight percent loadings	299:331	different weight percent loadings	299:331	The addition of Aloe vera extract at different weight percent loadings was investigated.
33947555	3	21	theme	scanning	472:479	arg1	SEM					502:504	SEM	502:504	SEM	502:504	The morphology, thermal properties, physical properties, and water absorption of the nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).
33947555	3	21	theme	scanning	472:479	arg1	microscopy					490:499	scanning electron microscopy	472:499	scanning electron microscopy (SEM)	472:505	The morphology, thermal properties, physical properties, and water absorption of the nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).
33947555	3	22	dep	Fourier	508:514	arg1	transform					516:524	transform	516:524	transform infrared spectroscopy (FTIR)	516:553	The morphology, thermal properties, physical properties, and water absorption of the nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).
33947555	2	23	theme	Aloe	278:281	arg1	extract					288:294	Aloe vera extract	278:294	Aloe vera extract	278:294	The addition of Aloe vera extract at different weight percent loadings was investigated.
33947555	1	24	theme	biodegradable	106:118	arg1	nanofibers					130:139	Natural, biocompatible, and biodegradable composite nanofibers	78:139	Natural, biocompatible, and biodegradable composite nanofibers made of Aloe vera extract, pullulan, chitosan, and citric acid	78:202	Natural, biocompatible, and biodegradable composite nanofibers made of Aloe vera extract, pullulan, chitosan, and citric acid were successfully produced via Forcespinning® technology.
33947555	1	25	theme	citric	192:197	arg1	acid					199:202	citric acid	192:202	citric acid	192:202	Natural, biocompatible, and biodegradable composite nanofibers made of Aloe vera extract, pullulan, chitosan, and citric acid were successfully produced via Forcespinning® technology.
33947555	3	26	theme	water	412:416	arg1	absorption					418:427	water absorption	412:427	water absorption	412:427	The morphology, thermal properties, physical properties, and water absorption of the nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).
33947555	5	27	theme	dressing	859:866	arg1	applications					868:879	wound dressing applications	853:879	wound dressing applications due to its ability to absorb excessive blood and exudates, as well as provide protection from infection while maintaining good thermal stability	853:1024	Its porous and high surface area structure make it a potential candidate for wound dressing applications due to its ability to absorb excessive blood and exudates, as well as provide protection from infection while maintaining good thermal stability.
33947555	4	28	theme	absorption	649:658	arg1	activity					700:707	synergistic antibacterial activity	674:707	synergistic antibacterial activity against Escherichia coli	674:732	The developed nanofiber membranes exhibited good water absorption capabilities, synergistic antibacterial activity against Escherichia coli, and promoted cell attachment and growth.
33947555	4	28	theme	absorption	649:658	arg1	capabilities					660:671	good water absorption capabilities	638:671	good water absorption capabilities	638:671	The developed nanofiber membranes exhibited good water absorption capabilities, synergistic antibacterial activity against Escherichia coli, and promoted cell attachment and growth.
33947555	5	29	theme	due	881:883	arg1	applications					868:879	wound dressing applications	853:879	wound dressing applications due to its ability to absorb excessive blood and exudates, as well as provide protection from infection while maintaining good thermal stability	853:1024	Its porous and high surface area structure make it a potential candidate for wound dressing applications due to its ability to absorb excessive blood and exudates, as well as provide protection from infection while maintaining good thermal stability.
33947555	5	30	dep	porous	780:785	arg1	structure					809:817	structure	809:817	structure	809:817	Its porous and high surface area structure make it a potential candidate for wound dressing applications due to its ability to absorb excessive blood and exudates, as well as provide protection from infection while maintaining good thermal stability.
33947555	5	31	theme	potential	829:837	arg1	candidate					839:847	a potential candidate	827:847	a potential candidate for wound dressing applications due to its ability to absorb excessive blood and exudates, as well as provide protection from infection while maintaining good thermal stability	827:1024	Its porous and high surface area structure make it a potential candidate for wound dressing applications due to its ability to absorb excessive blood and exudates, as well as provide protection from infection while maintaining good thermal stability.
33947555	4	32	theme	water	643:647	arg1	activity					700:707	synergistic antibacterial activity	674:707	synergistic antibacterial activity against Escherichia coli	674:732	The developed nanofiber membranes exhibited good water absorption capabilities, synergistic antibacterial activity against Escherichia coli, and promoted cell attachment and growth.
33947555	4	32	theme	water	643:647	arg1	capabilities					660:671	good water absorption capabilities	638:671	good water absorption capabilities	638:671	The developed nanofiber membranes exhibited good water absorption capabilities, synergistic antibacterial activity against Escherichia coli, and promoted cell attachment and growth.
33947555	0	33	theme	dressing	55:62	arg1	applications					64:75	wound dressing applications	49:75	wound dressing applications	49:75	Aloe Vera extract-based composite nanofibers for wound dressing applications.
33947555	1	34	theme	composite	120:128	arg1	nanofibers					130:139	Natural, biocompatible, and biodegradable composite nanofibers	78:139	Natural, biocompatible, and biodegradable composite nanofibers made of Aloe vera extract, pullulan, chitosan, and citric acid	78:202	Natural, biocompatible, and biodegradable composite nanofibers made of Aloe vera extract, pullulan, chitosan, and citric acid were successfully produced via Forcespinning® technology.
33947555	0	35	theme	wound	49:53	arg1	applications					64:75	wound dressing applications	49:75	wound dressing applications	49:75	Aloe Vera extract-based composite nanofibers for wound dressing applications.
33947555	3	36	theme	thermogravimetric	560:576	arg1	TGA					588:590	TGA	588:590	TGA	588:590	The morphology, thermal properties, physical properties, and water absorption of the nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).
33947555	3	36	theme	thermogravimetric	560:576	arg1	analysis					578:585	thermogravimetric analysis	560:585	thermogravimetric analysis (TGA)	560:591	The morphology, thermal properties, physical properties, and water absorption of the nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).
33947555	5	37	theme	good	1003:1006	arg1	stability					1016:1024	good thermal stability	1003:1024	good thermal stability	1003:1024	Its porous and high surface area structure make it a potential candidate for wound dressing applications due to its ability to absorb excessive blood and exudates, as well as provide protection from infection while maintaining good thermal stability.
33947555	4	38	theme	cell	748:751	arg1	attachment					753:762	cell attachment	748:762	cell attachment	748:762	The developed nanofiber membranes exhibited good water absorption capabilities, synergistic antibacterial activity against Escherichia coli, and promoted cell attachment and growth.
33947555	3	39	dep	transform	516:524	arg1	infrared					526:533	infrared	526:533	transform infrared spectroscopy (FTIR)	516:553	The morphology, thermal properties, physical properties, and water absorption of the nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).
33947555	4	40	theme	developed	598:606	arg1	membranes					618:626	The developed nanofiber membranes	594:626	The developed nanofiber membranes	594:626	The developed nanofiber membranes exhibited good water absorption capabilities, synergistic antibacterial activity against Escherichia coli, and promoted cell attachment and growth.
33947555	5	41	theme	thermal	1008:1014	arg1	stability					1016:1024	good thermal stability	1003:1024	good thermal stability	1003:1024	Its porous and high surface area structure make it a potential candidate for wound dressing applications due to its ability to absorb excessive blood and exudates, as well as provide protection from infection while maintaining good thermal stability.
33947555	4	42	theme	good	638:641	arg1	activity					700:707	synergistic antibacterial activity	674:707	synergistic antibacterial activity against Escherichia coli	674:732	The developed nanofiber membranes exhibited good water absorption capabilities, synergistic antibacterial activity against Escherichia coli, and promoted cell attachment and growth.
33947555	4	42	theme	good	638:641	arg1	capabilities					660:671	good water absorption capabilities	638:671	good water absorption capabilities	638:671	The developed nanofiber membranes exhibited good water absorption capabilities, synergistic antibacterial activity against Escherichia coli, and promoted cell attachment and growth.
33947555	1	43	theme	Aloe	149:152	arg1	extract					159:165	Aloe vera extract	149:165	Aloe vera extract	149:165	Natural, biocompatible, and biodegradable composite nanofibers made of Aloe vera extract, pullulan, chitosan, and citric acid were successfully produced via Forcespinning® technology.
33947555	4	44	theme	nanofiber	608:616	arg1	membranes					618:626	The developed nanofiber membranes	594:626	The developed nanofiber membranes	594:626	The developed nanofiber membranes exhibited good water absorption capabilities, synergistic antibacterial activity against Escherichia coli, and promoted cell attachment and growth.
33947555	1	45	theme	vera	154:157	arg1	extract					159:165	Aloe vera extract	149:165	Aloe vera extract	149:165	Natural, biocompatible, and biodegradable composite nanofibers made of Aloe vera extract, pullulan, chitosan, and citric acid were successfully produced via Forcespinning® technology.
33947555	1	46	theme	Forcespinning®	235:248	arg1	technology					250:259	Forcespinning® technology	235:259	Forcespinning® technology	235:259	Natural, biocompatible, and biodegradable composite nanofibers made of Aloe vera extract, pullulan, chitosan, and citric acid were successfully produced via Forcespinning® technology.
34757828	0	0	theme	Cellulose	82:90	arg1	Gel					92:94	Hydroxypropyl Cellulose Gel	68:94	Hydroxypropyl Cellulose Gel	68:94	Determination of Microstructural Impact on the Release of Drug from Hydroxypropyl Cellulose Gel by Validated In Vitro Release Test Method.
34757828	5	1	theme	in	781:782	arg1	method					803:808	a developed and validated in vitro release test method	755:808	a developed and validated in vitro release test method	755:808	The CHX G drug release study was performed using a developed and validated in vitro release test method, which is reproducible, discriminative, and robust to detect the formulation differences.
34757828	5	1	theme	in	781:782	arg1	reproducible					820:831	reproducible	820:831	reproducible	820:831	The CHX G drug release study was performed using a developed and validated in vitro release test method, which is reproducible, discriminative, and robust to detect the formulation differences.
34757828	5	2	theme	G	714:714	arg1	study					729:733	The CHX G drug release study	706:733	The CHX G drug release study	706:733	The CHX G drug release study was performed using a developed and validated in vitro release test method, which is reproducible, discriminative, and robust to detect the formulation differences.
34757828	2	3	theme	release	367:373	arg1	properties					375:384	the drug release properties	358:384	the drug release properties	358:384	Different polymers exhibit different three-dimensional structure and these have a great impact on the drug release properties.
34757828	5	4	theme	developed	757:765	arg1	method					803:808	a developed and validated in vitro release test method	755:808	a developed and validated in vitro release test method	755:808	The CHX G drug release study was performed using a developed and validated in vitro release test method, which is reproducible, discriminative, and robust to detect the formulation differences.
34757828	5	4	theme	developed	757:765	arg1	reproducible					820:831	reproducible	820:831	reproducible	820:831	The CHX G drug release study was performed using a developed and validated in vitro release test method, which is reproducible, discriminative, and robust to detect the formulation differences.
34757828	6	5	theme	formulations	1008:1019	arg1	rates					985:989	the release rates	973:989	the release rates of the different formulations	973:1019	The drug release results showed that there was appreciable difference in the release rates of the different formulations.
34757828	0	6	theme	Hydroxypropyl	68:80	arg1	Gel					92:94	Hydroxypropyl Cellulose Gel	68:94	Hydroxypropyl Cellulose Gel	68:94	Determination of Microstructural Impact on the Release of Drug from Hydroxypropyl Cellulose Gel by Validated In Vitro Release Test Method.
34757828	2	7	from	impact	348:353	arg1	properties					375:384	the drug release properties	358:384	the drug release properties	358:384	Different polymers exhibit different three-dimensional structure and these have a great impact on the drug release properties.
34757828	2	8	theme	drug	362:365	arg1	properties					375:384	the drug release properties	358:384	the drug release properties	358:384	Different polymers exhibit different three-dimensional structure and these have a great impact on the drug release properties.
34757828	4	9	theme	main	555:558	arg1	methods					574:580	The two main investigating methods	547:580	The two main investigating methods of microstructure	547:598	The two main investigating methods of microstructure were used namely, rheology and texture analysis to determine the differences in the formulations studied.
34757828	6	10	theme	different	998:1006	arg1	formulations					1008:1019	the different formulations	994:1019	the different formulations	994:1019	The drug release results showed that there was appreciable difference in the release rates of the different formulations.
34757828	8	11	theme	statistical	1176:1186	arg1	approach					1188:1195	a statistical approach	1174:1195	a statistical approach using analysis of variance	1174:1222	The formulations differences were further confirmed by a statistical approach using analysis of variance.
34757828	0	12	from	Determination	0:12	arg1	Release					47:53	the Release	43:53	the Release of Drug from Hydroxypropyl Cellulose Gel	43:94	Determination of Microstructural Impact on the Release of Drug from Hydroxypropyl Cellulose Gel by Validated In Vitro Release Test Method.
34757828	0	13	dep	In	109:110	arg1	Vitro					112:116	Vitro	112:116	Vitro	112:116	Determination of Microstructural Impact on the Release of Drug from Hydroxypropyl Cellulose Gel by Validated In Vitro Release Test Method.
34757828	5	14	dep	in	781:782	arg1	vitro					784:788	vitro	784:788	vitro	784:788	The CHX G drug release study was performed using a developed and validated in vitro release test method, which is reproducible, discriminative, and robust to detect the formulation differences.
34757828	6	15	theme	release	977:983	arg1	rates					985:989	the release rates	973:989	the release rates of the different formulations	973:1019	The drug release results showed that there was appreciable difference in the release rates of the different formulations.
34757828	6	16	theme	drug	904:907	arg1	results					917:923	The drug release results	900:923	The drug release results	900:923	The drug release results showed that there was appreciable difference in the release rates of the different formulations.
34757828	2	17	theme	three-dimensional	297:313	arg1	structure					315:323	different three-dimensional structure	287:323	different three-dimensional structure	287:323	Different polymers exhibit different three-dimensional structure and these have a great impact on the drug release properties.
34757828	7	18	from	difference	1086:1095	arg1	rates					1112:1116	the release rates	1100:1116	the release rates	1100:1116	The rheology and texture analysis data correlated well with the difference in the release rates.
34757828	0	19	theme	In	109:110	arg1	Method					131:136	Validated In Vitro Release Test Method	99:136	Validated In Vitro Release Test Method	99:136	Determination of Microstructural Impact on the Release of Drug from Hydroxypropyl Cellulose Gel by Validated In Vitro Release Test Method.
34757828	0	20	from	Release	47:53	arg1	Determination					0:12	Determination	0:12	Determination of Microstructural Impact on the Release of Drug from Hydroxypropyl Cellulose Gel by Validated In Vitro Release Test Method.	0:137	Determination of Microstructural Impact on the Release of Drug from Hydroxypropyl Cellulose Gel by Validated In Vitro Release Test Method.
34757828	7	21	theme	release	1104:1110	arg1	rates					1112:1116	the release rates	1100:1116	the release rates	1100:1116	The rheology and texture analysis data correlated well with the difference in the release rates.
34757828	2	22	theme	different	287:295	arg1	structure					315:323	different three-dimensional structure	287:323	different three-dimensional structure	287:323	Different polymers exhibit different three-dimensional structure and these have a great impact on the drug release properties.
34757828	8	23	theme	variance	1215:1222	arg1	analysis					1203:1210	analysis	1203:1210	analysis of variance	1203:1222	The formulations differences were further confirmed by a statistical approach using analysis of variance.
34757828	1	24	theme	semisolid	159:167	arg1	system					169:174	a semisolid system	157:174	a semisolid system	157:174	Microstructure of a semisolid system is greatly influenced by the formulation composition and the processing parameters.
34757828	0	25	theme	Validated	99:107	arg1	Method					131:136	Validated In Vitro Release Test Method	99:136	Validated In Vitro Release Test Method	99:136	Determination of Microstructural Impact on the Release of Drug from Hydroxypropyl Cellulose Gel by Validated In Vitro Release Test Method.
34757828	1	26	theme	system	169:174	arg1	Microstructure					139:152	Microstructure	139:152	Microstructure of a semisolid system	139:174	Microstructure of a semisolid system is greatly influenced by the formulation composition and the processing parameters.
34757828	4	27	dep	rheology	618:625	arg1	analysis					639:646	analysis	639:646	analysis	639:646	The two main investigating methods of microstructure were used namely, rheology and texture analysis to determine the differences in the formulations studied.
34757828	4	28	from	differences	665:675	arg1	formulations					684:695	the formulations	680:695	the formulations studied	680:703	The two main investigating methods of microstructure were used namely, rheology and texture analysis to determine the differences in the formulations studied.
34757828	3	29	theme	hydroxypropyl	442:454	arg1	microstructure					470:483	hydroxypropyl cellulose gel microstructure	442:483	hydroxypropyl cellulose gel microstructure	442:483	The current research focuses on studying the impact of hydroxypropyl cellulose gel microstructure on the release properties of chlorhexidine gluconate (CHX G).
34757828	0	30	theme	Impact	33:38	arg1	Determination					0:12	Determination	0:12	Determination of Microstructural Impact on the Release of Drug from Hydroxypropyl Cellulose Gel by Validated In Vitro Release Test Method.	0:137	Determination of Microstructural Impact on the Release of Drug from Hydroxypropyl Cellulose Gel by Validated In Vitro Release Test Method.
34757828	4	31	theme	investigating	560:572	arg1	methods					574:580	The two main investigating methods	547:580	The two main investigating methods of microstructure	547:598	The two main investigating methods of microstructure were used namely, rheology and texture analysis to determine the differences in the formulations studied.
34757828	0	32	theme	Release	118:124	arg1	Method					131:136	Validated In Vitro Release Test Method	99:136	Validated In Vitro Release Test Method	99:136	Determination of Microstructural Impact on the Release of Drug from Hydroxypropyl Cellulose Gel by Validated In Vitro Release Test Method.
34757828	0	33	theme	Microstructural	17:31	arg1	Impact					33:38	Microstructural Impact	17:38	Microstructural Impact on the Release of Drug from Hydroxypropyl Cellulose Gel	17:94	Determination of Microstructural Impact on the Release of Drug from Hydroxypropyl Cellulose Gel by Validated In Vitro Release Test Method.
34757828	4	34	theme	microstructure	585:598	arg1	methods					574:580	The two main investigating methods	547:580	The two main investigating methods of microstructure	547:598	The two main investigating methods of microstructure were used namely, rheology and texture analysis to determine the differences in the formulations studied.
34757828	5	35	theme	release	721:727	arg1	study					729:733	The CHX G drug release study	706:733	The CHX G drug release study	706:733	The CHX G drug release study was performed using a developed and validated in vitro release test method, which is reproducible, discriminative, and robust to detect the formulation differences.
34757828	3	36	theme	CHX	539:541	arg1	gluconate					528:536	chlorhexidine gluconate	514:536	chlorhexidine gluconate (CHX G)	514:544	The current research focuses on studying the impact of hydroxypropyl cellulose gel microstructure on the release properties of chlorhexidine gluconate (CHX G).
34757828	3	36	theme	CHX	539:541	arg1	G					543:543	CHX G	539:543	CHX G	539:543	The current research focuses on studying the impact of hydroxypropyl cellulose gel microstructure on the release properties of chlorhexidine gluconate (CHX G).
34757828	3	37	theme	release	492:498	arg1	properties					500:509	the release properties	488:509	the release properties of chlorhexidine gluconate (CHX G)	488:544	The current research focuses on studying the impact of hydroxypropyl cellulose gel microstructure on the release properties of chlorhexidine gluconate (CHX G).
34757828	5	38	theme	release	790:796	arg1	method					803:808	a developed and validated in vitro release test method	755:808	a developed and validated in vitro release test method	755:808	The CHX G drug release study was performed using a developed and validated in vitro release test method, which is reproducible, discriminative, and robust to detect the formulation differences.
34757828	5	38	theme	release	790:796	arg1	reproducible					820:831	reproducible	820:831	reproducible	820:831	The CHX G drug release study was performed using a developed and validated in vitro release test method, which is reproducible, discriminative, and robust to detect the formulation differences.
34757828	5	39	theme	formulation	875:885	arg1	differences					887:897	the formulation differences	871:897	the formulation differences	871:897	The CHX G drug release study was performed using a developed and validated in vitro release test method, which is reproducible, discriminative, and robust to detect the formulation differences.
34757828	3	40	theme	microstructure	470:483	arg1	impact					432:437	the impact	428:437	the impact of hydroxypropyl cellulose gel microstructure on the release properties of chlorhexidine gluconate (CHX G)	428:544	The current research focuses on studying the impact of hydroxypropyl cellulose gel microstructure on the release properties of chlorhexidine gluconate (CHX G).
34757828	3	41	theme	cellulose	456:464	arg1	microstructure					470:483	hydroxypropyl cellulose gel microstructure	442:483	hydroxypropyl cellulose gel microstructure	442:483	The current research focuses on studying the impact of hydroxypropyl cellulose gel microstructure on the release properties of chlorhexidine gluconate (CHX G).
34757828	0	42	theme	Test	126:129	arg1	Method					131:136	Validated In Vitro Release Test Method	99:136	Validated In Vitro Release Test Method	99:136	Determination of Microstructural Impact on the Release of Drug from Hydroxypropyl Cellulose Gel by Validated In Vitro Release Test Method.
34757828	7	43	theme	analysis	1047:1054	arg1	data					1056:1059	The rheology and texture analysis data	1022:1059	The rheology and texture analysis data	1022:1059	The rheology and texture analysis data correlated well with the difference in the release rates.
34757828	7	44	theme	rheology	1026:1033	arg1	data					1056:1059	The rheology and texture analysis data	1022:1059	The rheology and texture analysis data	1022:1059	The rheology and texture analysis data correlated well with the difference in the release rates.
34757828	1	45	theme	formulation	205:215	arg1	composition					217:227	the formulation composition	201:227	the formulation composition	201:227	Microstructure of a semisolid system is greatly influenced by the formulation composition and the processing parameters.
34757828	6	46	theme	appreciable	947:957	arg1	difference					959:968	appreciable difference	947:968	appreciable difference	947:968	The drug release results showed that there was appreciable difference in the release rates of the different formulations.
34757828	5	47	theme	validated	771:779	arg1	method					803:808	a developed and validated in vitro release test method	755:808	a developed and validated in vitro release test method	755:808	The CHX G drug release study was performed using a developed and validated in vitro release test method, which is reproducible, discriminative, and robust to detect the formulation differences.
34757828	5	47	theme	validated	771:779	arg1	reproducible					820:831	reproducible	820:831	reproducible	820:831	The CHX G drug release study was performed using a developed and validated in vitro release test method, which is reproducible, discriminative, and robust to detect the formulation differences.
34757828	7	48	theme	texture	1039:1045	arg1	data					1056:1059	The rheology and texture analysis data	1022:1059	The rheology and texture analysis data	1022:1059	The rheology and texture analysis data correlated well with the difference in the release rates.
34757828	3	49	theme	chlorhexidine	514:526	arg1	gluconate					528:536	chlorhexidine gluconate	514:536	chlorhexidine gluconate (CHX G)	514:544	The current research focuses on studying the impact of hydroxypropyl cellulose gel microstructure on the release properties of chlorhexidine gluconate (CHX G).
34757828	3	49	theme	chlorhexidine	514:526	arg1	G					543:543	CHX G	539:543	CHX G	539:543	The current research focuses on studying the impact of hydroxypropyl cellulose gel microstructure on the release properties of chlorhexidine gluconate (CHX G).
34757828	3	50	from	impact	432:437	arg1	properties					500:509	the release properties	488:509	the release properties of chlorhexidine gluconate (CHX G)	488:544	The current research focuses on studying the impact of hydroxypropyl cellulose gel microstructure on the release properties of chlorhexidine gluconate (CHX G).
34757828	2	51	contain	have	335:338	arg1	these					329:333	these	329:333	these	329:333	Different polymers exhibit different three-dimensional structure and these have a great impact on the drug release properties.
34757828	2	51	contain	have	335:338	arg2	impact					348:353	a great impact	340:353	a great impact on the drug release properties	340:384	Different polymers exhibit different three-dimensional structure and these have a great impact on the drug release properties.
34757828	5	52	theme	drug	716:719	arg1	study					729:733	The CHX G drug release study	706:733	The CHX G drug release study	706:733	The CHX G drug release study was performed using a developed and validated in vitro release test method, which is reproducible, discriminative, and robust to detect the formulation differences.
34757828	3	53	theme	gluconate	528:536	arg1	properties					500:509	the release properties	488:509	the release properties of chlorhexidine gluconate (CHX G)	488:544	The current research focuses on studying the impact of hydroxypropyl cellulose gel microstructure on the release properties of chlorhexidine gluconate (CHX G).
34757828	5	54	theme	CHX	710:712	arg1	study					729:733	The CHX G drug release study	706:733	The CHX G drug release study	706:733	The CHX G drug release study was performed using a developed and validated in vitro release test method, which is reproducible, discriminative, and robust to detect the formulation differences.
34757828	5	55	theme	test	798:801	arg1	method					803:808	a developed and validated in vitro release test method	755:808	a developed and validated in vitro release test method	755:808	The CHX G drug release study was performed using a developed and validated in vitro release test method, which is reproducible, discriminative, and robust to detect the formulation differences.
34757828	5	55	theme	test	798:801	arg1	reproducible					820:831	reproducible	820:831	reproducible	820:831	The CHX G drug release study was performed using a developed and validated in vitro release test method, which is reproducible, discriminative, and robust to detect the formulation differences.
34757828	8	56	theme	formulations	1123:1134	arg1	differences					1136:1146	The formulations differences	1119:1146	The formulations differences	1119:1146	The formulations differences were further confirmed by a statistical approach using analysis of variance.
34757828	4	57	used	used	605:608	arg2	methods					574:580	The two main investigating methods	547:580	The two main investigating methods of microstructure	547:598	The two main investigating methods of microstructure were used namely, rheology and texture analysis to determine the differences in the formulations studied.
34757828	2	58	theme	Different	260:268	arg1	polymers					270:277	Different polymers	260:277	Different polymers	260:277	Different polymers exhibit different three-dimensional structure and these have a great impact on the drug release properties.
34757828	2	59	theme	great	342:346	arg1	impact					348:353	a great impact	340:353	a great impact on the drug release properties	340:384	Different polymers exhibit different three-dimensional structure and these have a great impact on the drug release properties.
34757828	0	60	from	Gel	92:94	arg1	Release					47:53	the Release	43:53	the Release of Drug from Hydroxypropyl Cellulose Gel	43:94	Determination of Microstructural Impact on the Release of Drug from Hydroxypropyl Cellulose Gel by Validated In Vitro Release Test Method.
34757828	3	61	theme	gel	466:468	arg1	microstructure					470:483	hydroxypropyl cellulose gel microstructure	442:483	hydroxypropyl cellulose gel microstructure	442:483	The current research focuses on studying the impact of hydroxypropyl cellulose gel microstructure on the release properties of chlorhexidine gluconate (CHX G).
34757828	6	62	theme	release	909:915	arg1	results					917:923	The drug release results	900:923	The drug release results	900:923	The drug release results showed that there was appreciable difference in the release rates of the different formulations.
34757828	0	63	theme	Drug	58:61	arg1	Release					47:53	the Release	43:53	the Release of Drug from Hydroxypropyl Cellulose Gel	43:94	Determination of Microstructural Impact on the Release of Drug from Hydroxypropyl Cellulose Gel by Validated In Vitro Release Test Method.
34757828	0	64	from	Impact	33:38	arg1	Release					47:53	the Release	43:53	the Release of Drug from Hydroxypropyl Cellulose Gel	43:94	Determination of Microstructural Impact on the Release of Drug from Hydroxypropyl Cellulose Gel by Validated In Vitro Release Test Method.
34757828	3	65	theme	current	391:397	arg1	research					399:406	The current research	387:406	The current research	387:406	The current research focuses on studying the impact of hydroxypropyl cellulose gel microstructure on the release properties of chlorhexidine gluconate (CHX G).
34757828	1	66	theme	processing	237:246	arg1	parameters					248:257	the processing parameters	233:257	the processing parameters	233:257	Microstructure of a semisolid system is greatly influenced by the formulation composition and the processing parameters.
32746987	2	0	theme	low	521:523	arg1	rate					539:542	electrospinning and low cell adhesion rate	501:542	electrospinning and low cell adhesion rate	501:542	However, application of natural polymers, such as hyaluronic acid, is challenging in construction of scaffolds due to physicochemical properties, difficult to use in electrospinning and low cell adhesion rate.
32746987	8	1	theme	potential	1502:1510	arg1	application					1490:1500	application potential	1490:1510	application potential	1490:1510	Acetylated HA was biocompatible with chondroinductivity features, therefore it not only had the positive characteristics of natural HA, but also enhanced the cellular attachment and application potential.
32746987	2	2	theme	hyaluronic	385:394	arg1	acid					396:399	hyaluronic acid	385:399	hyaluronic acid	385:399	However, application of natural polymers, such as hyaluronic acid, is challenging in construction of scaffolds due to physicochemical properties, difficult to use in electrospinning and low cell adhesion rate.
32746987	6	3	theme	genes	1018:1022	arg1	Expression					985:994	Expression	985:994	Expression of cartilage-specific genes (SOX9, Col II and Aggrecan)	985:1050	Expression of cartilage-specific genes (SOX9, Col II and Aggrecan) was monitored by Real-time PCR.
32746987	0	4	theme	cells	97:101	arg1	differentiation					61:75	chondrogenic differentiation	48:75	chondrogenic differentiation of mesenchymal stem cells seeded on electrospun PCL scaffolds	48:137	Acetylated hyaluronic acid effectively enhances chondrogenic differentiation of mesenchymal stem cells seeded on electrospun PCL scaffolds.
32746987	6	5	dep	genes	1018:1022	arg1	Aggrecan					1042:1049	Aggrecan	1042:1049	Aggrecan	1042:1049	Expression of cartilage-specific genes (SOX9, Col II and Aggrecan) was monitored by Real-time PCR.
32746987	6	5	dep	genes	1018:1022	arg1	SOX9					1025:1028	SOX9	1025:1028	SOX9	1025:1028	Expression of cartilage-specific genes (SOX9, Col II and Aggrecan) was monitored by Real-time PCR.
32746987	6	5	dep	genes	1018:1022	arg1	genes					1018:1022	cartilage-specific genes	999:1022	cartilage-specific genes (SOX9, Col II and Aggrecan)	999:1050	Expression of cartilage-specific genes (SOX9, Col II and Aggrecan) was monitored by Real-time PCR.
32746987	6	5	dep	genes	1018:1022	arg1	Col					1031:1033	Col	1031:1033	Col	1031:1033	Expression of cartilage-specific genes (SOX9, Col II and Aggrecan) was monitored by Real-time PCR.
32746987	7	6	theme	Sox9	1122:1125	arg1	expression					1108:1117	expression	1108:1117	expression of Sox9 and Col II as the markers of chondrogenic differentiation as well as the results of Alcian blue staining,	1108:1231	Significant increase in expression of Sox9 and Col II as the markers of chondrogenic differentiation as well as the results of Alcian blue staining, indicated the chondro-inductive potential of HA/PCL nanofibrous scaffolds.
32746987	5	7	theme	scaffolds	909:917	arg1	scaffolds					909:917	the composite scaffolds	895:917	the composite scaffolds	895:917	The presence of acetyl groups, as well as morphology and biocompatibility of the composite scaffolds were characterized by HNMR, FTIR, SEM and MTT assay respectively.
32746987	5	7	theme	scaffolds	909:917	arg1	biocompatibility					875:890	biocompatibility	875:890	biocompatibility	875:890	The presence of acetyl groups, as well as morphology and biocompatibility of the composite scaffolds were characterized by HNMR, FTIR, SEM and MTT assay respectively.
32746987	5	7	theme	scaffolds	909:917	arg1	morphology					860:869	morphology	860:869	morphology	860:869	The presence of acetyl groups, as well as morphology and biocompatibility of the composite scaffolds were characterized by HNMR, FTIR, SEM and MTT assay respectively.
32746987	5	7	theme	scaffolds	909:917	arg1	groups					841:846	acetyl groups	834:846	acetyl groups	834:846	The presence of acetyl groups, as well as morphology and biocompatibility of the composite scaffolds were characterized by HNMR, FTIR, SEM and MTT assay respectively.
32746987	7	8	theme	staining	1223:1230	arg1	markers					1145:1151	the markers	1141:1151	the markers of chondrogenic differentiation	1141:1183	Significant increase in expression of Sox9 and Col II as the markers of chondrogenic differentiation as well as the results of Alcian blue staining, indicated the chondro-inductive potential of HA/PCL nanofibrous scaffolds.
32746987	7	8	theme	staining	1223:1230	arg1	results					1200:1206	the results	1196:1206	the results of Alcian blue staining	1196:1230	Significant increase in expression of Sox9 and Col II as the markers of chondrogenic differentiation as well as the results of Alcian blue staining, indicated the chondro-inductive potential of HA/PCL nanofibrous scaffolds.
32746987	6	9	theme	cartilage-specific	999:1016	arg1	Aggrecan					1042:1049	Aggrecan	1042:1049	Aggrecan	1042:1049	Expression of cartilage-specific genes (SOX9, Col II and Aggrecan) was monitored by Real-time PCR.
32746987	6	9	theme	cartilage-specific	999:1016	arg1	SOX9					1025:1028	SOX9	1025:1028	SOX9	1025:1028	Expression of cartilage-specific genes (SOX9, Col II and Aggrecan) was monitored by Real-time PCR.
32746987	6	9	theme	cartilage-specific	999:1016	arg1	genes					1018:1022	cartilage-specific genes	999:1022	cartilage-specific genes (SOX9, Col II and Aggrecan)	999:1050	Expression of cartilage-specific genes (SOX9, Col II and Aggrecan) was monitored by Real-time PCR.
32746987	6	9	theme	cartilage-specific	999:1016	arg1	Col					1031:1033	Col	1031:1033	Col	1031:1033	Expression of cartilage-specific genes (SOX9, Col II and Aggrecan) was monitored by Real-time PCR.
32746987	0	10	theme	electrospun	113:123	arg1	scaffolds					129:137	electrospun PCL scaffolds	113:137	electrospun PCL scaffolds	113:137	Acetylated hyaluronic acid effectively enhances chondrogenic differentiation of mesenchymal stem cells seeded on electrospun PCL scaffolds.
32746987	3	11	theme	scaffolds	697:705	arg1	construction					647:658	construction	647:658	construction of nanofibrous composite (ac-HA/PCL) scaffolds	647:705	In this study, HA was acetylated to make it soluble in high polarity solvent and blended with PCL for construction of nanofibrous composite (ac-HA/PCL) scaffolds.
32746987	4	12	theme	stem	797:800	arg1	hADSCs					809:814	hADSCs	809:814	hADSCs	809:814	Chondroinductivity of the constructed scaffolds was investigated using human mesenchymal stem cells (hADSCs).
32746987	4	12	theme	stem	797:800	arg1	cells					802:806	human mesenchymal stem cells	779:806	human mesenchymal stem cells (hADSCs)	779:815	Chondroinductivity of the constructed scaffolds was investigated using human mesenchymal stem cells (hADSCs).
32746987	2	13	from	use	494:496	arg1	rate					539:542	electrospinning and low cell adhesion rate	501:542	electrospinning and low cell adhesion rate	501:542	However, application of natural polymers, such as hyaluronic acid, is challenging in construction of scaffolds due to physicochemical properties, difficult to use in electrospinning and low cell adhesion rate.
32746987	4	14	theme	mesenchymal	785:795	arg1	hADSCs					809:814	hADSCs	809:814	hADSCs	809:814	Chondroinductivity of the constructed scaffolds was investigated using human mesenchymal stem cells (hADSCs).
32746987	4	14	theme	mesenchymal	785:795	arg1	cells					802:806	human mesenchymal stem cells	779:806	human mesenchymal stem cells (hADSCs)	779:815	Chondroinductivity of the constructed scaffolds was investigated using human mesenchymal stem cells (hADSCs).
32746987	3	15	from	solvent	614:620	arg1	soluble					589:595	soluble	589:595	soluble	589:595	In this study, HA was acetylated to make it soluble in high polarity solvent and blended with PCL for construction of nanofibrous composite (ac-HA/PCL) scaffolds.
32746987	2	16	theme	natural	359:365	arg1	acid					396:399	hyaluronic acid	385:399	hyaluronic acid	385:399	However, application of natural polymers, such as hyaluronic acid, is challenging in construction of scaffolds due to physicochemical properties, difficult to use in electrospinning and low cell adhesion rate.
32746987	2	16	theme	natural	359:365	arg1	polymers					367:374	natural polymers	359:374	natural polymers	359:374	However, application of natural polymers, such as hyaluronic acid, is challenging in construction of scaffolds due to physicochemical properties, difficult to use in electrospinning and low cell adhesion rate.
32746987	7	17	theme	differentiation	1169:1183	arg1	markers					1145:1151	the markers	1141:1151	the markers of chondrogenic differentiation	1141:1183	Significant increase in expression of Sox9 and Col II as the markers of chondrogenic differentiation as well as the results of Alcian blue staining, indicated the chondro-inductive potential of HA/PCL nanofibrous scaffolds.
32746987	7	17	theme	differentiation	1169:1183	arg1	results					1200:1206	the results	1196:1206	the results of Alcian blue staining	1196:1230	Significant increase in expression of Sox9 and Col II as the markers of chondrogenic differentiation as well as the results of Alcian blue staining, indicated the chondro-inductive potential of HA/PCL nanofibrous scaffolds.
32746987	7	18	theme	Significant	1084:1094	arg1	increase					1096:1103	Significant increase	1084:1103	Significant increase in expression of Sox9 and Col II as the markers of chondrogenic differentiation as well as the results of Alcian blue staining,	1084:1231	Significant increase in expression of Sox9 and Col II as the markers of chondrogenic differentiation as well as the results of Alcian blue staining, indicated the chondro-inductive potential of HA/PCL nanofibrous scaffolds.
32746987	6	19	theme	Real-time	1069:1077	arg1	PCR					1079:1081	Real-time PCR	1069:1081	Real-time PCR	1069:1081	Expression of cartilage-specific genes (SOX9, Col II and Aggrecan) was monitored by Real-time PCR.
32746987	1	20	theme	natural	187:193	arg1	niches					195:200	natural niches	187:200	natural niches regarding both biochemical composition and mechanical characteristics	187:270	Construction of scaffolds which are similar to natural niches regarding both biochemical composition and mechanical characteristics has gained great attention in the field of tissue engineering.
32746987	0	21	theme	PCL	125:127	arg1	scaffolds					129:137	electrospun PCL scaffolds	113:137	electrospun PCL scaffolds	113:137	Acetylated hyaluronic acid effectively enhances chondrogenic differentiation of mesenchymal stem cells seeded on electrospun PCL scaffolds.
32746987	2	22	theme	electrospinning	501:515	arg1	rate					539:542	electrospinning and low cell adhesion rate	501:542	electrospinning and low cell adhesion rate	501:542	However, application of natural polymers, such as hyaluronic acid, is challenging in construction of scaffolds due to physicochemical properties, difficult to use in electrospinning and low cell adhesion rate.
32746987	7	23	theme	chondrogenic	1156:1167	arg1	differentiation					1169:1183	chondrogenic differentiation	1156:1183	chondrogenic differentiation	1156:1183	Significant increase in expression of Sox9 and Col II as the markers of chondrogenic differentiation as well as the results of Alcian blue staining, indicated the chondro-inductive potential of HA/PCL nanofibrous scaffolds.
32746987	8	24	theme	cellular	1466:1473	arg1	attachment					1475:1484	the cellular attachment	1462:1484	the cellular attachment	1462:1484	Acetylated HA was biocompatible with chondroinductivity features, therefore it not only had the positive characteristics of natural HA, but also enhanced the cellular attachment and application potential.
32746987	2	25	theme	difficult	481:489	arg1	properties					469:478	physicochemical properties	453:478	physicochemical properties	453:478	However, application of natural polymers, such as hyaluronic acid, is challenging in construction of scaffolds due to physicochemical properties, difficult to use in electrospinning and low cell adhesion rate.
32746987	7	26	from	increase	1096:1103	arg1	expression					1108:1117	expression	1108:1117	expression of Sox9 and Col II as the markers of chondrogenic differentiation as well as the results of Alcian blue staining,	1108:1231	Significant increase in expression of Sox9 and Col II as the markers of chondrogenic differentiation as well as the results of Alcian blue staining, indicated the chondro-inductive potential of HA/PCL nanofibrous scaffolds.
32746987	0	27	theme	stem	92:95	arg1	cells					97:101	mesenchymal stem cells	80:101	mesenchymal stem cells seeded on electrospun PCL scaffolds	80:137	Acetylated hyaluronic acid effectively enhances chondrogenic differentiation of mesenchymal stem cells seeded on electrospun PCL scaffolds.
32746987	7	28	theme	chondro-inductive	1247:1263	arg1	potential					1265:1273	the chondro-inductive potential	1243:1273	the chondro-inductive potential of HA/PCL nanofibrous scaffolds	1243:1305	Significant increase in expression of Sox9 and Col II as the markers of chondrogenic differentiation as well as the results of Alcian blue staining, indicated the chondro-inductive potential of HA/PCL nanofibrous scaffolds.
32746987	0	29	theme	Acetylated	0:9	arg1	acid					22:25	Acetylated hyaluronic acid	0:25	Acetylated hyaluronic acid	0:25	Acetylated hyaluronic acid effectively enhances chondrogenic differentiation of mesenchymal stem cells seeded on electrospun PCL scaffolds.
32746987	4	30	theme	constructed	734:744	arg1	scaffolds					746:754	the constructed scaffolds	730:754	the constructed scaffolds	730:754	Chondroinductivity of the constructed scaffolds was investigated using human mesenchymal stem cells (hADSCs).
32746987	3	31	from	soluble	589:595	arg1	solvent					614:620	high polarity solvent	600:620	high polarity solvent	600:620	In this study, HA was acetylated to make it soluble in high polarity solvent and blended with PCL for construction of nanofibrous composite (ac-HA/PCL) scaffolds.
32746987	5	32	theme	acetyl	834:839	arg1	scaffolds					909:917	the composite scaffolds	895:917	the composite scaffolds	895:917	The presence of acetyl groups, as well as morphology and biocompatibility of the composite scaffolds were characterized by HNMR, FTIR, SEM and MTT assay respectively.
32746987	5	32	theme	acetyl	834:839	arg1	groups					841:846	acetyl groups	834:846	acetyl groups	834:846	The presence of acetyl groups, as well as morphology and biocompatibility of the composite scaffolds were characterized by HNMR, FTIR, SEM and MTT assay respectively.
32746987	1	33	theme	tissue	315:320	arg1	engineering					322:332	tissue engineering	315:332	tissue engineering	315:332	Construction of scaffolds which are similar to natural niches regarding both biochemical composition and mechanical characteristics has gained great attention in the field of tissue engineering.
32746987	4	34	theme	human	779:783	arg1	hADSCs					809:814	hADSCs	809:814	hADSCs	809:814	Chondroinductivity of the constructed scaffolds was investigated using human mesenchymal stem cells (hADSCs).
32746987	4	34	theme	human	779:783	arg1	cells					802:806	human mesenchymal stem cells	779:806	human mesenchymal stem cells (hADSCs)	779:815	Chondroinductivity of the constructed scaffolds was investigated using human mesenchymal stem cells (hADSCs).
32746987	1	35	theme	biochemical	217:227	arg1	composition					229:239	biochemical composition	217:239	biochemical composition	217:239	Construction of scaffolds which are similar to natural niches regarding both biochemical composition and mechanical characteristics has gained great attention in the field of tissue engineering.
32746987	2	36	theme	physicochemical	453:467	arg1	properties					469:478	physicochemical properties	453:478	physicochemical properties	453:478	However, application of natural polymers, such as hyaluronic acid, is challenging in construction of scaffolds due to physicochemical properties, difficult to use in electrospinning and low cell adhesion rate.
32746987	1	37	theme	engineering	322:332	arg1	field					306:310	the field	302:310	the field of tissue engineering	302:332	Construction of scaffolds which are similar to natural niches regarding both biochemical composition and mechanical characteristics has gained great attention in the field of tissue engineering.
32746987	2	38	theme	due	446:448	arg1	scaffolds					436:444	scaffolds	436:444	scaffolds due to physicochemical properties, difficult to use in electrospinning and low cell adhesion rate	436:542	However, application of natural polymers, such as hyaluronic acid, is challenging in construction of scaffolds due to physicochemical properties, difficult to use in electrospinning and low cell adhesion rate.
32746987	0	39	theme	chondrogenic	48:59	arg1	differentiation					61:75	chondrogenic differentiation	48:75	chondrogenic differentiation of mesenchymal stem cells seeded on electrospun PCL scaffolds	48:137	Acetylated hyaluronic acid effectively enhances chondrogenic differentiation of mesenchymal stem cells seeded on electrospun PCL scaffolds.
32746987	7	40	theme	nanofibrous	1285:1295	arg1	scaffolds					1297:1305	HA/PCL nanofibrous scaffolds	1278:1305	HA/PCL nanofibrous scaffolds	1278:1305	Significant increase in expression of Sox9 and Col II as the markers of chondrogenic differentiation as well as the results of Alcian blue staining, indicated the chondro-inductive potential of HA/PCL nanofibrous scaffolds.
32746987	7	41	theme	HA/PCL	1278:1283	arg1	scaffolds					1297:1305	HA/PCL nanofibrous scaffolds	1278:1305	HA/PCL nanofibrous scaffolds	1278:1305	Significant increase in expression of Sox9 and Col II as the markers of chondrogenic differentiation as well as the results of Alcian blue staining, indicated the chondro-inductive potential of HA/PCL nanofibrous scaffolds.
32746987	3	42	theme	high	600:603	arg1	solvent					614:620	high polarity solvent	600:620	high polarity solvent	600:620	In this study, HA was acetylated to make it soluble in high polarity solvent and blended with PCL for construction of nanofibrous composite (ac-HA/PCL) scaffolds.
32746987	2	43	theme	scaffolds	436:444	arg1	construction					420:431	construction	420:431	construction of scaffolds due to physicochemical properties, difficult to use in electrospinning and low cell adhesion rate	420:542	However, application of natural polymers, such as hyaluronic acid, is challenging in construction of scaffolds due to physicochemical properties, difficult to use in electrospinning and low cell adhesion rate.
32746987	5	44	theme	morphology	860:869	arg1	presence					822:829	The presence	818:829	The presence of acetyl groups, as well as morphology and biocompatibility of the composite scaffolds	818:917	The presence of acetyl groups, as well as morphology and biocompatibility of the composite scaffolds were characterized by HNMR, FTIR, SEM and MTT assay respectively.
32746987	3	45	theme	polarity	605:612	arg1	solvent					614:620	high polarity solvent	600:620	high polarity solvent	600:620	In this study, HA was acetylated to make it soluble in high polarity solvent and blended with PCL for construction of nanofibrous composite (ac-HA/PCL) scaffolds.
32746987	1	46	theme	mechanical	245:254	arg1	characteristics					256:270	mechanical characteristics	245:270	mechanical characteristics	245:270	Construction of scaffolds which are similar to natural niches regarding both biochemical composition and mechanical characteristics has gained great attention in the field of tissue engineering.
32746987	8	47	contain	had	1396:1398	arg1	it					1384:1385	it	1384:1385	it	1384:1385	Acetylated HA was biocompatible with chondroinductivity features, therefore it not only had the positive characteristics of natural HA, but also enhanced the cellular attachment and application potential.
32746987	8	47	contain	had	1396:1398	arg2	characteristics					1413:1427	the positive characteristics	1400:1427	the positive characteristics of natural HA	1400:1441	Acetylated HA was biocompatible with chondroinductivity features, therefore it not only had the positive characteristics of natural HA, but also enhanced the cellular attachment and application potential.
32746987	8	48	theme	chondroinductivity	1345:1362	arg1	features					1364:1371	chondroinductivity features	1345:1371	chondroinductivity features	1345:1371	Acetylated HA was biocompatible with chondroinductivity features, therefore it not only had the positive characteristics of natural HA, but also enhanced the cellular attachment and application potential.
32746987	2	49	theme	cell	525:528	arg1	rate					539:542	electrospinning and low cell adhesion rate	501:542	electrospinning and low cell adhesion rate	501:542	However, application of natural polymers, such as hyaluronic acid, is challenging in construction of scaffolds due to physicochemical properties, difficult to use in electrospinning and low cell adhesion rate.
32746987	8	50	theme	Acetylated	1308:1317	arg1	HA					1319:1320	Acetylated HA	1308:1320	Acetylated HA	1308:1320	Acetylated HA was biocompatible with chondroinductivity features, therefore it not only had the positive characteristics of natural HA, but also enhanced the cellular attachment and application potential.
32746987	7	51	theme	Col	1131:1133	arg1	expression					1108:1117	expression	1108:1117	expression of Sox9 and Col II as the markers of chondrogenic differentiation as well as the results of Alcian blue staining,	1108:1231	Significant increase in expression of Sox9 and Col II as the markers of chondrogenic differentiation as well as the results of Alcian blue staining, indicated the chondro-inductive potential of HA/PCL nanofibrous scaffolds.
32746987	8	52	with	biocompatible	1326:1338	arg1	features					1364:1371	chondroinductivity features	1345:1371	chondroinductivity features	1345:1371	Acetylated HA was biocompatible with chondroinductivity features, therefore it not only had the positive characteristics of natural HA, but also enhanced the cellular attachment and application potential.
32746987	8	53	theme	positive	1404:1411	arg1	characteristics					1413:1427	the positive characteristics	1400:1427	the positive characteristics of natural HA	1400:1441	Acetylated HA was biocompatible with chondroinductivity features, therefore it not only had the positive characteristics of natural HA, but also enhanced the cellular attachment and application potential.
32746987	8	54	theme	HA	1440:1441	arg1	characteristics					1413:1427	the positive characteristics	1400:1427	the positive characteristics of natural HA	1400:1441	Acetylated HA was biocompatible with chondroinductivity features, therefore it not only had the positive characteristics of natural HA, but also enhanced the cellular attachment and application potential.
32746987	3	55	theme	ac-HA/PCL	686:694	arg1	scaffolds					697:705	nanofibrous composite (ac-HA/PCL) scaffolds	663:705	nanofibrous composite (ac-HA/PCL) scaffolds	663:705	In this study, HA was acetylated to make it soluble in high polarity solvent and blended with PCL for construction of nanofibrous composite (ac-HA/PCL) scaffolds.
32746987	8	56	theme	natural	1432:1438	arg1	HA					1440:1441	natural HA	1432:1441	natural HA	1432:1441	Acetylated HA was biocompatible with chondroinductivity features, therefore it not only had the positive characteristics of natural HA, but also enhanced the cellular attachment and application potential.
32746987	2	57	theme	polymers	367:374	arg1	application					344:354	application	344:354	application of natural polymers, such as hyaluronic acid,	344:400	However, application of natural polymers, such as hyaluronic acid, is challenging in construction of scaffolds due to physicochemical properties, difficult to use in electrospinning and low cell adhesion rate.
32746987	3	58	theme	nanofibrous	663:673	arg1	scaffolds					697:705	nanofibrous composite (ac-HA/PCL) scaffolds	663:705	nanofibrous composite (ac-HA/PCL) scaffolds	663:705	In this study, HA was acetylated to make it soluble in high polarity solvent and blended with PCL for construction of nanofibrous composite (ac-HA/PCL) scaffolds.
32746987	5	59	theme	MTT	961:963	arg1	assay					965:969	MTT assay	961:969	MTT assay	961:969	The presence of acetyl groups, as well as morphology and biocompatibility of the composite scaffolds were characterized by HNMR, FTIR, SEM and MTT assay respectively.
32746987	1	60	theme	scaffolds	156:164	arg1	Construction					140:151	Construction	140:151	Construction of scaffolds which are similar to natural niches regarding both biochemical composition and mechanical characteristics	140:270	Construction of scaffolds which are similar to natural niches regarding both biochemical composition and mechanical characteristics has gained great attention in the field of tissue engineering.
32746987	4	61	theme	scaffolds	746:754	arg1	Chondroinductivity					708:725	Chondroinductivity	708:725	Chondroinductivity of the constructed scaffolds	708:754	Chondroinductivity of the constructed scaffolds was investigated using human mesenchymal stem cells (hADSCs).
32746987	2	62	theme	adhesion	530:537	arg1	rate					539:542	electrospinning and low cell adhesion rate	501:542	electrospinning and low cell adhesion rate	501:542	However, application of natural polymers, such as hyaluronic acid, is challenging in construction of scaffolds due to physicochemical properties, difficult to use in electrospinning and low cell adhesion rate.
32746987	3	63	theme	composite	675:683	arg1	scaffolds					697:705	nanofibrous composite (ac-HA/PCL) scaffolds	663:705	nanofibrous composite (ac-HA/PCL) scaffolds	663:705	In this study, HA was acetylated to make it soluble in high polarity solvent and blended with PCL for construction of nanofibrous composite (ac-HA/PCL) scaffolds.
32746987	7	64	theme	scaffolds	1297:1305	arg1	potential					1265:1273	the chondro-inductive potential	1243:1273	the chondro-inductive potential of HA/PCL nanofibrous scaffolds	1243:1305	Significant increase in expression of Sox9 and Col II as the markers of chondrogenic differentiation as well as the results of Alcian blue staining, indicated the chondro-inductive potential of HA/PCL nanofibrous scaffolds.
32746987	5	65	theme	composite	899:907	arg1	scaffolds					909:917	the composite scaffolds	895:917	the composite scaffolds	895:917	The presence of acetyl groups, as well as morphology and biocompatibility of the composite scaffolds were characterized by HNMR, FTIR, SEM and MTT assay respectively.
32746987	7	66	theme	Alcian	1211:1216	arg1	staining					1223:1230	Alcian blue staining	1211:1230	Alcian blue staining	1211:1230	Significant increase in expression of Sox9 and Col II as the markers of chondrogenic differentiation as well as the results of Alcian blue staining, indicated the chondro-inductive potential of HA/PCL nanofibrous scaffolds.
32746987	0	67	theme	mesenchymal	80:90	arg1	cells					97:101	mesenchymal stem cells	80:101	mesenchymal stem cells seeded on electrospun PCL scaffolds	80:137	Acetylated hyaluronic acid effectively enhances chondrogenic differentiation of mesenchymal stem cells seeded on electrospun PCL scaffolds.
32746987	5	68	theme	groups	841:846	arg1	presence					822:829	The presence	818:829	The presence of acetyl groups, as well as morphology and biocompatibility of the composite scaffolds	818:917	The presence of acetyl groups, as well as morphology and biocompatibility of the composite scaffolds were characterized by HNMR, FTIR, SEM and MTT assay respectively.
32746987	0	69	theme	hyaluronic	11:20	arg1	acid					22:25	Acetylated hyaluronic acid	0:25	Acetylated hyaluronic acid	0:25	Acetylated hyaluronic acid effectively enhances chondrogenic differentiation of mesenchymal stem cells seeded on electrospun PCL scaffolds.
32746987	7	70	theme	blue	1218:1221	arg1	staining					1223:1230	Alcian blue staining	1211:1230	Alcian blue staining	1211:1230	Significant increase in expression of Sox9 and Col II as the markers of chondrogenic differentiation as well as the results of Alcian blue staining, indicated the chondro-inductive potential of HA/PCL nanofibrous scaffolds.
32746987	1	71	theme	great	283:287	arg1	attention					289:297	great attention	283:297	great attention	283:297	Construction of scaffolds which are similar to natural niches regarding both biochemical composition and mechanical characteristics has gained great attention in the field of tissue engineering.
33910743	5	0	theme	ultralow	788:795	arg1	∼8.4 mg/cm3					806:816	∼8.4 mg/cm3	806:816	∼8.4 mg/cm3	806:816	The CNF/CS composite aerogel has ultralow density (∼8.4 mg/cm3), high temperature-invariant (above 300 °C) and high porosity (98 %).
33910743	5	0	theme	ultralow	788:795	arg1	density					797:803	ultralow density	788:803	ultralow density (∼8.4 mg/cm3)	788:817	The CNF/CS composite aerogel has ultralow density (∼8.4 mg/cm3), high temperature-invariant (above 300 °C) and high porosity (98 %).
33910743	4	1	theme	60	724:725	arg1	%					727:727	%	727:727	%	727:727	The chitosan was performed as strength polymers to prohibits the shrinkage and retains the structural stability of 3D cellulose nanofiber skeleton, endowing the composite aerogel with satisfactory deformation recovery ability (without loss under 60 % stress cycled 100 times).
33910743	6	2	from	36 mW m-1 K-1	1032:1044	arg1	direction					1018:1026	rational direction	1009:1026	rational direction	1009:1026	The CNF/CS aerogel demonstrates anisotropic thermal insulation properties with low thermal conductivity (28 mWm-1 K-1 in rational direction and 36 mW m-1 K-1 in the axial direction).
33910743	6	2	from	36 mW m-1 K-1	1032:1044	arg1	direction					1059:1067	the axial direction	1049:1067	the axial direction	1049:1067	The CNF/CS aerogel demonstrates anisotropic thermal insulation properties with low thermal conductivity (28 mWm-1 K-1 in rational direction and 36 mW m-1 K-1 in the axial direction).
33910743	8	3	theme	oil	1364:1366	arg1	applications					1379:1390	oil absorption applications	1364:1390	oil absorption applications	1364:1390	Therefore, the multifunctional CNF/CS composite aerogels are potential materials for thermal management and oil absorption applications.
33910743	5	4	theme	composite	766:774	arg1	aerogel					776:782	The CNF/CS composite aerogel	755:782	The CNF/CS composite aerogel	755:782	The CNF/CS composite aerogel has ultralow density (∼8.4 mg/cm3), high temperature-invariant (above 300 °C) and high porosity (98 %).
33910743	4	5	theme	nanofiber	606:614	arg1	skeleton					616:623	3D cellulose nanofiber skeleton	593:623	3D cellulose nanofiber skeleton	593:623	The chitosan was performed as strength polymers to prohibits the shrinkage and retains the structural stability of 3D cellulose nanofiber skeleton, endowing the composite aerogel with satisfactory deformation recovery ability (without loss under 60 % stress cycled 100 times).
33910743	5	6	contain	has	784:786	arg2	%					884:884	98 %	881:884	98 %	881:884	The CNF/CS composite aerogel has ultralow density (∼8.4 mg/cm3), high temperature-invariant (above 300 °C) and high porosity (98 %).
33910743	5	6	contain	has	784:786	arg2	porosity					871:878	high porosity	866:878	high porosity (98 %)	866:885	The CNF/CS composite aerogel has ultralow density (∼8.4 mg/cm3), high temperature-invariant (above 300 °C) and high porosity (98 %).
33910743	5	6	contain	has	784:786	arg1	aerogel					776:782	The CNF/CS composite aerogel	755:782	The CNF/CS composite aerogel	755:782	The CNF/CS composite aerogel has ultralow density (∼8.4 mg/cm3), high temperature-invariant (above 300 °C) and high porosity (98 %).
33910743	5	6	contain	has	784:786	arg2	∼8.4 mg/cm3					806:816	∼8.4 mg/cm3	806:816	∼8.4 mg/cm3	806:816	The CNF/CS composite aerogel has ultralow density (∼8.4 mg/cm3), high temperature-invariant (above 300 °C) and high porosity (98 %).
33910743	5	6	contain	has	784:786	arg2	°C					858:859	above 300 °C	848:859	above 300 °C	848:859	The CNF/CS composite aerogel has ultralow density (∼8.4 mg/cm3), high temperature-invariant (above 300 °C) and high porosity (98 %).
33910743	5	6	contain	has	784:786	arg2	density					797:803	ultralow density	788:803	ultralow density (∼8.4 mg/cm3)	788:817	The CNF/CS composite aerogel has ultralow density (∼8.4 mg/cm3), high temperature-invariant (above 300 °C) and high porosity (98 %).
33910743	5	6	contain	has	784:786	arg2	temperature-invariant					825:845	high temperature-invariant	820:845	high temperature-invariant (above 300 °C)	820:860	The CNF/CS composite aerogel has ultralow density (∼8.4 mg/cm3), high temperature-invariant (above 300 °C) and high porosity (98 %).
33910743	1	7	theme	cellulose-based	162:176	arg1	aerogels					178:185	cellulose-based aerogels	162:185	cellulose-based aerogels	162:185	Attributed to low cost, renewable, and high availability, cellulose-based aerogels are desirable materials for various applications.
33910743	1	7	theme	cellulose-based	162:176	arg1	materials					201:209	desirable materials	191:209	desirable materials for various applications	191:234	Attributed to low cost, renewable, and high availability, cellulose-based aerogels are desirable materials for various applications.
33910743	4	8	theme	cellulose	596:604	arg1	skeleton					616:623	3D cellulose nanofiber skeleton	593:623	3D cellulose nanofiber skeleton	593:623	The chitosan was performed as strength polymers to prohibits the shrinkage and retains the structural stability of 3D cellulose nanofiber skeleton, endowing the composite aerogel with satisfactory deformation recovery ability (without loss under 60 % stress cycled 100 times).
33910743	7	9	theme	high	1179:1182	arg1	capacity					1195:1202	high absorption capacity	1179:1202	high absorption capacity	1179:1202	Moreover, the composite aerogel (water contact angle ∼148°) exhibited outstanding oil/water selectivity and high absorption capacity (82-253 g/g) for various oils and organic solvents.
33910743	6	10	theme	CNF/CS	892:897	arg1	aerogel					899:905	The CNF/CS aerogel	888:905	The CNF/CS aerogel	888:905	The CNF/CS aerogel demonstrates anisotropic thermal insulation properties with low thermal conductivity (28 mWm-1 K-1 in rational direction and 36 mW m-1 K-1 in the axial direction).
33910743	7	11	theme	angle	1118:1122	arg1	∼148°					1124:1128	water contact angle ∼148°	1104:1128	water contact angle ∼148°	1104:1128	Moreover, the composite aerogel (water contact angle ∼148°) exhibited outstanding oil/water selectivity and high absorption capacity (82-253 g/g) for various oils and organic solvents.
33910743	7	11	theme	angle	1118:1122	arg1	aerogel					1095:1101	the composite aerogel	1081:1101	the composite aerogel (water contact angle ∼148°)	1081:1129	Moreover, the composite aerogel (water contact angle ∼148°) exhibited outstanding oil/water selectivity and high absorption capacity (82-253 g/g) for various oils and organic solvents.
33910743	7	12	theme	various	1221:1227	arg1	oils					1229:1232	various oils	1221:1232	various oils	1221:1232	Moreover, the composite aerogel (water contact angle ∼148°) exhibited outstanding oil/water selectivity and high absorption capacity (82-253 g/g) for various oils and organic solvents.
33910743	8	13	theme	absorption	1368:1377	arg1	applications					1379:1390	oil absorption applications	1364:1390	oil absorption applications	1364:1390	Therefore, the multifunctional CNF/CS composite aerogels are potential materials for thermal management and oil absorption applications.
33910743	4	14	theme	skeleton	616:623	arg1	stability					580:588	the structural stability	565:588	the structural stability of 3D cellulose nanofiber skeleton	565:623	The chitosan was performed as strength polymers to prohibits the shrinkage and retains the structural stability of 3D cellulose nanofiber skeleton, endowing the composite aerogel with satisfactory deformation recovery ability (without loss under 60 % stress cycled 100 times).
33910743	5	15	theme	CNF/CS	759:764	arg1	aerogel					776:782	The CNF/CS composite aerogel	755:782	The CNF/CS composite aerogel	755:782	The CNF/CS composite aerogel has ultralow density (∼8.4 mg/cm3), high temperature-invariant (above 300 °C) and high porosity (98 %).
33910743	7	16	theme	water	1104:1108	arg1	∼148°					1124:1128	water contact angle ∼148°	1104:1128	water contact angle ∼148°	1104:1128	Moreover, the composite aerogel (water contact angle ∼148°) exhibited outstanding oil/water selectivity and high absorption capacity (82-253 g/g) for various oils and organic solvents.
33910743	7	16	theme	water	1104:1108	arg1	aerogel					1095:1101	the composite aerogel	1081:1101	the composite aerogel (water contact angle ∼148°)	1081:1129	Moreover, the composite aerogel (water contact angle ∼148°) exhibited outstanding oil/water selectivity and high absorption capacity (82-253 g/g) for various oils and organic solvents.
33910743	8	17	theme	multifunctional	1271:1285	arg1	materials					1327:1335	potential materials	1317:1335	potential materials for thermal management and oil absorption applications	1317:1390	Therefore, the multifunctional CNF/CS composite aerogels are potential materials for thermal management and oil absorption applications.
33910743	8	17	theme	multifunctional	1271:1285	arg1	aerogels					1304:1311	the multifunctional CNF/CS composite aerogels	1267:1311	the multifunctional CNF/CS composite aerogels	1267:1311	Therefore, the multifunctional CNF/CS composite aerogels are potential materials for thermal management and oil absorption applications.
33910743	3	18	theme	chemical	449:456	arg1	process					469:475	chemical cross-link process	449:475	chemical cross-link process	449:475	Herein, we synthesized a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel via directional freeze casting and chemical cross-link process.
33910743	3	19	theme	CNF	385:387	arg1	aerogel					406:412	a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel	337:412	a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel via directional freeze casting and chemical cross-link process	337:475	Herein, we synthesized a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel via directional freeze casting and chemical cross-link process.
33910743	4	20	theme	strength	508:515	arg1	polymers					517:524	strength polymers	508:524	strength polymers	508:524	The chitosan was performed as strength polymers to prohibits the shrinkage and retains the structural stability of 3D cellulose nanofiber skeleton, endowing the composite aerogel with satisfactory deformation recovery ability (without loss under 60 % stress cycled 100 times).
33910743	4	20	theme	strength	508:515	arg1	chitosan					482:489	The chitosan	478:489	The chitosan	478:489	The chitosan was performed as strength polymers to prohibits the shrinkage and retains the structural stability of 3D cellulose nanofiber skeleton, endowing the composite aerogel with satisfactory deformation recovery ability (without loss under 60 % stress cycled 100 times).
33910743	0	21	theme	thermal	54:60	arg1	management					62:71	thermal management	54:71	thermal management	54:71	Anisotropic cellulose nanofiber/chitosan aerogel with thermal management and oil absorption properties.
33910743	3	22	theme	cross-link	458:467	arg1	process					469:475	chemical cross-link process	449:475	chemical cross-link process	449:475	Herein, we synthesized a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel via directional freeze casting and chemical cross-link process.
33910743	5	23	theme	high	820:823	arg1	temperature-invariant					825:845	high temperature-invariant	820:845	high temperature-invariant (above 300 °C)	820:860	The CNF/CS composite aerogel has ultralow density (∼8.4 mg/cm3), high temperature-invariant (above 300 °C) and high porosity (98 %).
33910743	5	23	theme	high	820:823	arg1	°C					858:859	above 300 °C	848:859	above 300 °C	848:859	The CNF/CS composite aerogel has ultralow density (∼8.4 mg/cm3), high temperature-invariant (above 300 °C) and high porosity (98 %).
33910743	0	24	theme	absorption	81:90	arg1	properties					92:101	oil absorption properties	77:101	oil absorption properties	77:101	Anisotropic cellulose nanofiber/chitosan aerogel with thermal management and oil absorption properties.
33910743	1	25	theme	desirable	191:199	arg1	aerogels					178:185	cellulose-based aerogels	162:185	cellulose-based aerogels	162:185	Attributed to low cost, renewable, and high availability, cellulose-based aerogels are desirable materials for various applications.
33910743	1	25	theme	desirable	191:199	arg1	materials					201:209	desirable materials	191:209	desirable materials for various applications	191:234	Attributed to low cost, renewable, and high availability, cellulose-based aerogels are desirable materials for various applications.
33910743	0	26	theme	Anisotropic	0:10	arg1	cellulose					12:20	Anisotropic cellulose	0:20	Anisotropic cellulose	0:20	Anisotropic cellulose nanofiber/chitosan aerogel with thermal management and oil absorption properties.
33910743	6	27	dep	conductivity	979:990	arg1	36 mW m-1 K-1					1032:1044	36 mW m-1 K-1	1032:1044	36 mW m-1 K-1 in the axial direction	1032:1067	The CNF/CS aerogel demonstrates anisotropic thermal insulation properties with low thermal conductivity (28 mWm-1 K-1 in rational direction and 36 mW m-1 K-1 in the axial direction).
33910743	6	27	dep	conductivity	979:990	arg1	28 mWm-1 K-1					993:1004	28 mWm-1 K-1	993:1004	28 mWm-1 K-1 in rational direction	993:1026	The CNF/CS aerogel demonstrates anisotropic thermal insulation properties with low thermal conductivity (28 mWm-1 K-1 in rational direction and 36 mW m-1 K-1 in the axial direction).
33910743	5	28	theme	high	866:869	arg1	porosity					871:878	high porosity	866:878	high porosity (98 %)	866:885	The CNF/CS composite aerogel has ultralow density (∼8.4 mg/cm3), high temperature-invariant (above 300 °C) and high porosity (98 %).
33910743	5	28	theme	high	866:869	arg1	%					884:884	98 %	881:884	98 %	881:884	The CNF/CS composite aerogel has ultralow density (∼8.4 mg/cm3), high temperature-invariant (above 300 °C) and high porosity (98 %).
33910743	3	29	theme	chitosan	392:399	arg1	aerogel					406:412	a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel	337:412	a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel via directional freeze casting and chemical cross-link process	337:475	Herein, we synthesized a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel via directional freeze casting and chemical cross-link process.
33910743	4	30	theme	structural	569:578	arg1	stability					580:588	the structural stability	565:588	the structural stability of 3D cellulose nanofiber skeleton	565:623	The chitosan was performed as strength polymers to prohibits the shrinkage and retains the structural stability of 3D cellulose nanofiber skeleton, endowing the composite aerogel with satisfactory deformation recovery ability (without loss under 60 % stress cycled 100 times).
33910743	6	31	theme	thermal	971:977	arg1	conductivity					979:990	low thermal conductivity	967:990	low thermal conductivity (28 mWm-1 K-1 in rational direction and 36 mW m-1 K-1 in the axial direction)	967:1068	The CNF/CS aerogel demonstrates anisotropic thermal insulation properties with low thermal conductivity (28 mWm-1 K-1 in rational direction and 36 mW m-1 K-1 in the axial direction).
33910743	1	32	theme	low	118:120	arg1	cost					122:125	low cost	118:125	low cost	118:125	Attributed to low cost, renewable, and high availability, cellulose-based aerogels are desirable materials for various applications.
33910743	7	33	theme	contact	1110:1116	arg1	∼148°					1124:1128	water contact angle ∼148°	1104:1128	water contact angle ∼148°	1104:1128	Moreover, the composite aerogel (water contact angle ∼148°) exhibited outstanding oil/water selectivity and high absorption capacity (82-253 g/g) for various oils and organic solvents.
33910743	7	33	theme	contact	1110:1116	arg1	aerogel					1095:1101	the composite aerogel	1081:1101	the composite aerogel (water contact angle ∼148°)	1081:1129	Moreover, the composite aerogel (water contact angle ∼148°) exhibited outstanding oil/water selectivity and high absorption capacity (82-253 g/g) for various oils and organic solvents.
33910743	4	34	theme	satisfactory	662:673	arg1	ability					696:702	satisfactory deformation recovery ability	662:702	satisfactory deformation recovery ability	662:702	The chitosan was performed as strength polymers to prohibits the shrinkage and retains the structural stability of 3D cellulose nanofiber skeleton, endowing the composite aerogel with satisfactory deformation recovery ability (without loss under 60 % stress cycled 100 times).
33910743	7	35	theme	absorption	1184:1193	arg1	capacity					1195:1202	high absorption capacity	1179:1202	high absorption capacity	1179:1202	Moreover, the composite aerogel (water contact angle ∼148°) exhibited outstanding oil/water selectivity and high absorption capacity (82-253 g/g) for various oils and organic solvents.
33910743	4	36	theme	3D	593:594	arg1	skeleton					616:623	3D cellulose nanofiber skeleton	593:623	3D cellulose nanofiber skeleton	593:623	The chitosan was performed as strength polymers to prohibits the shrinkage and retains the structural stability of 3D cellulose nanofiber skeleton, endowing the composite aerogel with satisfactory deformation recovery ability (without loss under 60 % stress cycled 100 times).
33910743	7	37	theme	composite	1085:1093	arg1	∼148°					1124:1128	water contact angle ∼148°	1104:1128	water contact angle ∼148°	1104:1128	Moreover, the composite aerogel (water contact angle ∼148°) exhibited outstanding oil/water selectivity and high absorption capacity (82-253 g/g) for various oils and organic solvents.
33910743	7	37	theme	composite	1085:1093	arg1	aerogel					1095:1101	the composite aerogel	1081:1101	the composite aerogel (water contact angle ∼148°)	1081:1129	Moreover, the composite aerogel (water contact angle ∼148°) exhibited outstanding oil/water selectivity and high absorption capacity (82-253 g/g) for various oils and organic solvents.
33910743	4	38	theme	%	727:727	arg1	stress					729:734	60 % stress	724:734	60 % stress	724:734	The chitosan was performed as strength polymers to prohibits the shrinkage and retains the structural stability of 3D cellulose nanofiber skeleton, endowing the composite aerogel with satisfactory deformation recovery ability (without loss under 60 % stress cycled 100 times).
33910743	1	39	theme	various	215:221	arg1	applications					223:234	various applications	215:234	various applications	215:234	Attributed to low cost, renewable, and high availability, cellulose-based aerogels are desirable materials for various applications.
33910743	3	40	theme	directional	418:428	arg1	casting					437:443	directional freeze casting	418:443	directional freeze casting	418:443	Herein, we synthesized a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel via directional freeze casting and chemical cross-link process.
33910743	6	41	theme	low	967:969	arg1	conductivity					979:990	low thermal conductivity	967:990	low thermal conductivity (28 mWm-1 K-1 in rational direction and 36 mW m-1 K-1 in the axial direction)	967:1068	The CNF/CS aerogel demonstrates anisotropic thermal insulation properties with low thermal conductivity (28 mWm-1 K-1 in rational direction and 36 mW m-1 K-1 in the axial direction).
33910743	4	42	theme	recovery	687:694	arg1	ability					696:702	satisfactory deformation recovery ability	662:702	satisfactory deformation recovery ability	662:702	The chitosan was performed as strength polymers to prohibits the shrinkage and retains the structural stability of 3D cellulose nanofiber skeleton, endowing the composite aerogel with satisfactory deformation recovery ability (without loss under 60 % stress cycled 100 times).
33910743	3	43	theme	freeze	430:435	arg1	casting					437:443	directional freeze casting	418:443	directional freeze casting	418:443	Herein, we synthesized a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel via directional freeze casting and chemical cross-link process.
33910743	6	44	theme	rational	1009:1016	arg1	direction					1018:1026	rational direction	1009:1026	rational direction	1009:1026	The CNF/CS aerogel demonstrates anisotropic thermal insulation properties with low thermal conductivity (28 mWm-1 K-1 in rational direction and 36 mW m-1 K-1 in the axial direction).
33910743	2	45	theme	mechanical	246:255	arg1	robustness					257:266	robustness	257:266	robustness	257:266	However, mechanical robustness and functionalization remain huge challenges.
33910743	3	46	dep	recoverable	339:349	arg1	anisotropic					352:362	anisotropic	352:362	anisotropic	352:362	Herein, we synthesized a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel via directional freeze casting and chemical cross-link process.
33910743	7	47	theme	outstanding	1141:1151	arg1	selectivity					1163:1173	outstanding oil/water selectivity	1141:1173	outstanding oil/water selectivity	1141:1173	Moreover, the composite aerogel (water contact angle ∼148°) exhibited outstanding oil/water selectivity and high absorption capacity (82-253 g/g) for various oils and organic solvents.
33910743	7	47	theme	outstanding	1141:1151	arg1	82-253 g/g					1205:1214	82-253 g/g	1205:1214	82-253 g/g	1205:1214	Moreover, the composite aerogel (water contact angle ∼148°) exhibited outstanding oil/water selectivity and high absorption capacity (82-253 g/g) for various oils and organic solvents.
33910743	6	48	theme	axial	1053:1057	arg1	direction					1059:1067	the axial direction	1049:1067	the axial direction	1049:1067	The CNF/CS aerogel demonstrates anisotropic thermal insulation properties with low thermal conductivity (28 mWm-1 K-1 in rational direction and 36 mW m-1 K-1 in the axial direction).
33910743	0	49	theme	oil	77:79	arg1	properties					92:101	oil absorption properties	77:101	oil absorption properties	77:101	Anisotropic cellulose nanofiber/chitosan aerogel with thermal management and oil absorption properties.
33910743	8	50	theme	potential	1317:1325	arg1	materials					1327:1335	potential materials	1317:1335	potential materials for thermal management and oil absorption applications	1317:1390	Therefore, the multifunctional CNF/CS composite aerogels are potential materials for thermal management and oil absorption applications.
33910743	8	50	theme	potential	1317:1325	arg1	aerogels					1304:1311	the multifunctional CNF/CS composite aerogels	1267:1311	the multifunctional CNF/CS composite aerogels	1267:1311	Therefore, the multifunctional CNF/CS composite aerogels are potential materials for thermal management and oil absorption applications.
33910743	7	51	theme	oil/water	1153:1161	arg1	selectivity					1163:1173	outstanding oil/water selectivity	1141:1173	outstanding oil/water selectivity	1141:1173	Moreover, the composite aerogel (water contact angle ∼148°) exhibited outstanding oil/water selectivity and high absorption capacity (82-253 g/g) for various oils and organic solvents.
33910743	7	51	theme	oil/water	1153:1161	arg1	82-253 g/g					1205:1214	82-253 g/g	1205:1214	82-253 g/g	1205:1214	Moreover, the composite aerogel (water contact angle ∼148°) exhibited outstanding oil/water selectivity and high absorption capacity (82-253 g/g) for various oils and organic solvents.
33910743	6	52	theme	insulation	940:949	arg1	properties					951:960	anisotropic thermal insulation properties	920:960	anisotropic thermal insulation properties	920:960	The CNF/CS aerogel demonstrates anisotropic thermal insulation properties with low thermal conductivity (28 mWm-1 K-1 in rational direction and 36 mW m-1 K-1 in the axial direction).
33910743	3	53	theme	recoverable	339:349	arg1	aerogel					406:412	a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel	337:412	a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel via directional freeze casting and chemical cross-link process	337:475	Herein, we synthesized a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel via directional freeze casting and chemical cross-link process.
33910743	6	54	from	28 mWm-1 K-1	993:1004	arg1	direction					1018:1026	rational direction	1009:1026	rational direction	1009:1026	The CNF/CS aerogel demonstrates anisotropic thermal insulation properties with low thermal conductivity (28 mWm-1 K-1 in rational direction and 36 mW m-1 K-1 in the axial direction).
33910743	6	54	from	28 mWm-1 K-1	993:1004	arg1	direction					1059:1067	the axial direction	1049:1067	the axial direction	1049:1067	The CNF/CS aerogel demonstrates anisotropic thermal insulation properties with low thermal conductivity (28 mWm-1 K-1 in rational direction and 36 mW m-1 K-1 in the axial direction).
33910743	7	55	theme	organic	1238:1244	arg1	solvents					1246:1253	organic solvents	1238:1253	organic solvents	1238:1253	Moreover, the composite aerogel (water contact angle ∼148°) exhibited outstanding oil/water selectivity and high absorption capacity (82-253 g/g) for various oils and organic solvents.
33910743	6	56	theme	thermal	932:938	arg1	properties					951:960	anisotropic thermal insulation properties	920:960	anisotropic thermal insulation properties	920:960	The CNF/CS aerogel demonstrates anisotropic thermal insulation properties with low thermal conductivity (28 mWm-1 K-1 in rational direction and 36 mW m-1 K-1 in the axial direction).
33910743	2	57	theme	huge	297:300	arg1	challenges					302:311	huge challenges	297:311	huge challenges	297:311	However, mechanical robustness and functionalization remain huge challenges.
33910743	8	58	theme	composite	1294:1302	arg1	materials					1327:1335	potential materials	1317:1335	potential materials for thermal management and oil absorption applications	1317:1390	Therefore, the multifunctional CNF/CS composite aerogels are potential materials for thermal management and oil absorption applications.
33910743	8	58	theme	composite	1294:1302	arg1	aerogels					1304:1311	the multifunctional CNF/CS composite aerogels	1267:1311	the multifunctional CNF/CS composite aerogels	1267:1311	Therefore, the multifunctional CNF/CS composite aerogels are potential materials for thermal management and oil absorption applications.
33910743	3	59	theme	cellulose	364:372	arg1	aerogel					406:412	a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel	337:412	a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel via directional freeze casting and chemical cross-link process	337:475	Herein, we synthesized a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel via directional freeze casting and chemical cross-link process.
33910743	4	60	theme	deformation	675:685	arg1	ability					696:702	satisfactory deformation recovery ability	662:702	satisfactory deformation recovery ability	662:702	The chitosan was performed as strength polymers to prohibits the shrinkage and retains the structural stability of 3D cellulose nanofiber skeleton, endowing the composite aerogel with satisfactory deformation recovery ability (without loss under 60 % stress cycled 100 times).
33910743	8	61	theme	CNF/CS	1287:1292	arg1	materials					1327:1335	potential materials	1317:1335	potential materials for thermal management and oil absorption applications	1317:1390	Therefore, the multifunctional CNF/CS composite aerogels are potential materials for thermal management and oil absorption applications.
33910743	8	61	theme	CNF/CS	1287:1292	arg1	aerogels					1304:1311	the multifunctional CNF/CS composite aerogels	1267:1311	the multifunctional CNF/CS composite aerogels	1267:1311	Therefore, the multifunctional CNF/CS composite aerogels are potential materials for thermal management and oil absorption applications.
33910743	1	62	theme	high	143:146	arg1	availability					148:159	high availability	143:159	high availability	143:159	Attributed to low cost, renewable, and high availability, cellulose-based aerogels are desirable materials for various applications.
33910743	3	63	theme	nanofiber	374:382	arg1	aerogel					406:412	a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel	337:412	a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel via directional freeze casting and chemical cross-link process	337:475	Herein, we synthesized a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel via directional freeze casting and chemical cross-link process.
33910743	6	64	theme	anisotropic	920:930	arg1	properties					951:960	anisotropic thermal insulation properties	920:960	anisotropic thermal insulation properties	920:960	The CNF/CS aerogel demonstrates anisotropic thermal insulation properties with low thermal conductivity (28 mWm-1 K-1 in rational direction and 36 mW m-1 K-1 in the axial direction).
33910743	8	65	theme	thermal	1341:1347	arg1	management					1349:1358	thermal management	1341:1358	thermal management	1341:1358	Therefore, the multifunctional CNF/CS composite aerogels are potential materials for thermal management and oil absorption applications.
33910743	4	66	theme	composite	639:647	arg1	aerogel					649:655	the composite aerogel	635:655	the composite aerogel	635:655	The chitosan was performed as strength polymers to prohibits the shrinkage and retains the structural stability of 3D cellulose nanofiber skeleton, endowing the composite aerogel with satisfactory deformation recovery ability (without loss under 60 % stress cycled 100 times).
33310258	0	0	theme	Willow	68:73	arg1	Aphid					75:79	a Giant Willow Aphid	60:79	a Giant Willow Aphid (Tuberolachnus salignus)	60:104	Composition and potential as a prebiotic functional food of a Giant Willow Aphid (Tuberolachnus salignus) honeydew honey produced in New Zealand.
33310258	5	1	theme	human-small-intestine	997:1017	arg1	simulations					1019:1029	crude human-stomach and human-small-intestine simulations	973:1029	crude human-stomach and human-small-intestine simulations	973:1029	Melezitose extracted from GWA honeydew honey was not significantly hydrolyzed in crude human-stomach and human-small-intestine simulations and may therefore meet the prebiotic criterion of human indigestibility.
33310258	1	2	theme	invasive	205:212	arg1	pest					214:217	an invasive pest	202:217	an invasive pest insect in New Zealand, which excretes honeydew	202:264	The Giant Willow Aphid (Tuberolachnus salignus, GWA) is an invasive pest insect in New Zealand, which excretes honeydew.
33310258	1	2	theme	invasive	205:212	arg1	Aphid					163:167	The Giant Willow Aphid	146:167	The Giant Willow Aphid (Tuberolachnus salignus, GWA)	146:197	The Giant Willow Aphid (Tuberolachnus salignus, GWA) is an invasive pest insect in New Zealand, which excretes honeydew.
33310258	0	3	theme	Giant	62:66	arg1	Aphid					75:79	a Giant Willow Aphid	60:79	a Giant Willow Aphid (Tuberolachnus salignus)	60:104	Composition and potential as a prebiotic functional food of a Giant Willow Aphid (Tuberolachnus salignus) honeydew honey produced in New Zealand.
33310258	4	4	theme	%	776:776	arg1	monosaccharides					778:792	41.2% monosaccharides	772:792	41.2% monosaccharides (lower than typical honeydew honeys)	772:829	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	4	5	gly	carbohydrate	554:565	arg1	GWA					578:580	GWA	578:580	GWA	578:580	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	3	6	theme	oligosaccharides	483:498	arg1	concentrations					465:478	high concentrations	460:478	high concentrations of oligosaccharides, particularly melezitose	460:523	This crystallization has been ascribed to high concentrations of oligosaccharides, particularly melezitose.
33310258	1	7	theme	insect	219:224	arg1	pest					214:217	an invasive pest	202:217	an invasive pest insect in New Zealand, which excretes honeydew	202:264	The Giant Willow Aphid (Tuberolachnus salignus, GWA) is an invasive pest insect in New Zealand, which excretes honeydew.
33310258	1	7	theme	insect	219:224	arg1	Aphid					163:167	The Giant Willow Aphid	146:167	The Giant Willow Aphid (Tuberolachnus salignus, GWA)	146:197	The Giant Willow Aphid (Tuberolachnus salignus, GWA) is an invasive pest insect in New Zealand, which excretes honeydew.
33310258	0	8	theme	Aphid	75:79	arg1	food					52:55	a prebiotic functional food	29:55	a prebiotic functional food of a Giant Willow Aphid (Tuberolachnus salignus)	29:104	Composition and potential as a prebiotic functional food of a Giant Willow Aphid (Tuberolachnus salignus) honeydew honey produced in New Zealand.
33310258	5	9	theme	crude	973:977	arg1	simulations					1019:1029	crude human-stomach and human-small-intestine simulations	973:1029	crude human-stomach and human-small-intestine simulations	973:1029	Melezitose extracted from GWA honeydew honey was not significantly hydrolyzed in crude human-stomach and human-small-intestine simulations and may therefore meet the prebiotic criterion of human indigestibility.
33310258	4	10	theme	37.8	650:653	arg1	%					654:654	%	654:654	%	654:654	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	4	11	theme	%	841:841	arg1	acid					853:856	0.054% salicylic acid	836:856	0.054% salicylic acid (higher than previous estimates)	836:889	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	5	12	theme	human-stomach	979:991	arg1	simulations					1019:1029	crude human-stomach and human-small-intestine simulations	973:1029	crude human-stomach and human-small-intestine simulations	973:1029	Melezitose extracted from GWA honeydew honey was not significantly hydrolyzed in crude human-stomach and human-small-intestine simulations and may therefore meet the prebiotic criterion of human indigestibility.
33310258	4	13	theme	typical	746:752	arg1	honeys					763:768	typical honeydew honeys	746:768	typical honeydew honeys	746:768	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	4	14	theme	oligosaccharides	662:677	arg1	melezitose					698:707	melezitose	698:707	melezitose	698:707	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	1	15	theme	Willow	156:161	arg1	pest					214:217	an invasive pest	202:217	an invasive pest insect in New Zealand, which excretes honeydew	202:264	The Giant Willow Aphid (Tuberolachnus salignus, GWA) is an invasive pest insect in New Zealand, which excretes honeydew.
33310258	1	15	theme	Willow	156:161	arg1	GWA					194:196	GWA	194:196	GWA	194:196	The Giant Willow Aphid (Tuberolachnus salignus, GWA) is an invasive pest insect in New Zealand, which excretes honeydew.
33310258	1	15	theme	Willow	156:161	arg1	Aphid					163:167	The Giant Willow Aphid	146:167	The Giant Willow Aphid (Tuberolachnus salignus, GWA)	146:197	The Giant Willow Aphid (Tuberolachnus salignus, GWA) is an invasive pest insect in New Zealand, which excretes honeydew.
33310258	2	16	theme	honey	335:339	arg1	type					327:330	a type	325:330	a type of honey that crystallizes in the comb, representing a significant loss to apiarists	325:415	European honeybees collect this honeydew and make it into a type of honey that crystallizes in the comb, representing a significant loss to apiarists.
33310258	0	17	theme	functional	41:50	arg1	food					52:55	a prebiotic functional food	29:55	a prebiotic functional food of a Giant Willow Aphid (Tuberolachnus salignus)	29:104	Composition and potential as a prebiotic functional food of a Giant Willow Aphid (Tuberolachnus salignus) honeydew honey produced in New Zealand.
33310258	4	18	theme	previous	871:878	arg1	estimates					880:888	previous estimates	871:888	previous estimates	871:888	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	0	19	dep	Aphid	75:79	arg1	salignus					96:103	Tuberolachnus salignus	82:103	Tuberolachnus salignus	82:103	Composition and potential as a prebiotic functional food of a Giant Willow Aphid (Tuberolachnus salignus) honeydew honey produced in New Zealand.
33310258	1	20	theme	Giant	150:154	arg1	pest					214:217	an invasive pest	202:217	an invasive pest insect in New Zealand, which excretes honeydew	202:264	The Giant Willow Aphid (Tuberolachnus salignus, GWA) is an invasive pest insect in New Zealand, which excretes honeydew.
33310258	1	20	theme	Giant	150:154	arg1	GWA					194:196	GWA	194:196	GWA	194:196	The Giant Willow Aphid (Tuberolachnus salignus, GWA) is an invasive pest insect in New Zealand, which excretes honeydew.
33310258	1	20	theme	Giant	150:154	arg1	Aphid					163:167	The Giant Willow Aphid	146:167	The Giant Willow Aphid (Tuberolachnus salignus, GWA)	146:197	The Giant Willow Aphid (Tuberolachnus salignus, GWA) is an invasive pest insect in New Zealand, which excretes honeydew.
33310258	4	21	dep	monosaccharides	778:792	arg1	lower					795:799	lower	795:799	lower	795:799	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	2	22	theme	European	267:274	arg1	honeybees					276:284	European honeybees	267:284	European honeybees	267:284	European honeybees collect this honeydew and make it into a type of honey that crystallizes in the comb, representing a significant loss to apiarists.
33310258	4	23	theme	GWA	610:612	arg1	honey					623:627	GWA honeydew honey	610:627	GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose	610:707	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	1	24	theme	New	229:231	arg1	Zealand					233:239	New Zealand	229:239	New Zealand	229:239	The Giant Willow Aphid (Tuberolachnus salignus, GWA) is an invasive pest insect in New Zealand, which excretes honeydew.
33310258	1	25	from	Zealand	233:239	arg1	insect					219:224	insect	219:224	insect	219:224	The Giant Willow Aphid (Tuberolachnus salignus, GWA) is an invasive pest insect in New Zealand, which excretes honeydew.
33310258	4	26	theme	total	656:660	arg1	oligosaccharides					662:677	37.8% total oligosaccharides	650:677	37.8% total oligosaccharides of which 27.4% was melezitose	650:707	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	5	27	theme	human	1081:1085	arg1	indigestibility					1087:1101	human indigestibility	1081:1101	human indigestibility	1081:1101	Melezitose extracted from GWA honeydew honey was not significantly hydrolyzed in crude human-stomach and human-small-intestine simulations and may therefore meet the prebiotic criterion of human indigestibility.
33310258	1	28	from	insect	219:224	arg1	Zealand					233:239	New Zealand	229:239	New Zealand	229:239	The Giant Willow Aphid (Tuberolachnus salignus, GWA) is an invasive pest insect in New Zealand, which excretes honeydew.
33310258	5	29	theme	indigestibility	1087:1101	arg1	criterion					1068:1076	the prebiotic criterion	1054:1076	the prebiotic criterion of human indigestibility	1054:1101	Melezitose extracted from GWA honeydew honey was not significantly hydrolyzed in crude human-stomach and human-small-intestine simulations and may therefore meet the prebiotic criterion of human indigestibility.
33310258	4	30	theme	typical	806:812	arg1	honeys					823:828	typical honeydew honeys	806:828	typical honeydew honeys	806:828	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	4	31	theme	GWA	578:580	arg1	profile					567:573	the first carbohydrate profile	544:573	the first carbohydrate profile of GWA	544:580	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	0	32	theme	prebiotic	31:39	arg1	food					52:55	a prebiotic functional food	29:55	a prebiotic functional food of a Giant Willow Aphid (Tuberolachnus salignus)	29:104	Composition and potential as a prebiotic functional food of a Giant Willow Aphid (Tuberolachnus salignus) honeydew honey produced in New Zealand.
33310258	4	33	theme	gluconic	719:726	arg1	acid					728:731	gluconic acid	719:731	gluconic acid	719:731	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	4	34	theme	0.054	836:840	arg1	%					841:841	%	841:841	%	841:841	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	0	35	theme	New	133:135	arg1	Zealand					137:143	New Zealand	133:143	New Zealand	133:143	Composition and potential as a prebiotic functional food of a Giant Willow Aphid (Tuberolachnus salignus) honeydew honey produced in New Zealand.
33310258	4	36	theme	honeydew	754:761	arg1	honeys					763:768	typical honeydew honeys	746:768	typical honeydew honeys	746:768	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	2	37	theme	significant	387:397	arg1	loss					399:402	a significant loss	385:402	a significant loss to apiarists	385:415	European honeybees collect this honeydew and make it into a type of honey that crystallizes in the comb, representing a significant loss to apiarists.
33310258	5	38	theme	prebiotic	1058:1066	arg1	criterion					1068:1076	the prebiotic criterion	1054:1076	the prebiotic criterion of human indigestibility	1054:1101	Melezitose extracted from GWA honeydew honey was not significantly hydrolyzed in crude human-stomach and human-small-intestine simulations and may therefore meet the prebiotic criterion of human indigestibility.
33310258	4	39	theme	honeydew	814:821	arg1	honeys					823:828	typical honeydew honeys	806:828	typical honeydew honeys	806:828	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	5	40	theme	GWA	918:920	arg1	honey					931:935	GWA honeydew honey	918:935	GWA honeydew honey	918:935	Melezitose extracted from GWA honeydew honey was not significantly hydrolyzed in crude human-stomach and human-small-intestine simulations and may therefore meet the prebiotic criterion of human indigestibility.
33310258	4	41	dep	acid	853:856	arg1	higher					859:864	higher	859:864	higher	859:864	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	5	42	theme	honeydew	922:929	arg1	honey					931:935	GWA honeydew honey	918:935	GWA honeydew honey	918:935	Melezitose extracted from GWA honeydew honey was not significantly hydrolyzed in crude human-stomach and human-small-intestine simulations and may therefore meet the prebiotic criterion of human indigestibility.
33310258	4	43	dep	honey	591:595	arg1	acid					728:731	gluconic acid	719:731	gluconic acid	719:731	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	4	43	dep	honey	591:595	arg1	higher					734:739	higher	734:739	higher	734:739	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	4	44	theme	%	654:654	arg1	oligosaccharides					662:677	37.8% total oligosaccharides	650:677	37.8% total oligosaccharides of which 27.4% was melezitose	650:707	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	4	45	theme	carbohydrate	554:565	arg1	profile					567:573	the first carbohydrate profile	544:573	the first carbohydrate profile of GWA	544:580	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	4	46	theme	honeydew	614:621	arg1	honey					623:627	GWA honeydew honey	610:627	GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose	610:707	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	4	47	theme	first	548:552	arg1	profile					567:573	the first carbohydrate profile	544:573	the first carbohydrate profile of GWA	544:580	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	4	48	theme	honey	623:627	arg1	%					717:717	2.5%	714:717	2.5%	714:717	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	4	48	theme	honey	623:627	arg1	honey					591:595	honey	591:595	honey	591:595	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	4	48	theme	honey	623:627	arg1	acid					853:856	0.054% salicylic acid	836:856	0.054% salicylic acid (higher than previous estimates)	836:889	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	4	48	theme	honey	623:627	arg1	monosaccharides					778:792	41.2% monosaccharides	772:792	41.2% monosaccharides (lower than typical honeydew honeys)	772:829	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	4	48	theme	honey	623:627	arg1	honey					623:627	GWA honeydew honey	610:627	GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose	610:707	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	4	48	theme	honey	623:627	arg1	sample					600:605	a sample	598:605	a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose	598:707	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	4	49	theme	salicylic	843:851	arg1	acid					853:856	0.054% salicylic acid	836:856	0.054% salicylic acid (higher than previous estimates)	836:889	In this research, the first carbohydrate profile of GWA honeydew honey, a sample of GWA honeydew honey was found to contain 37.8% total oligosaccharides of which 27.4% was melezitose, and 2.5% gluconic acid (higher than typical honeydew honeys); 41.2% monosaccharides (lower than typical honeydew honeys); and 0.054% salicylic acid (higher than previous estimates).
33310258	3	50	theme	high	460:463	arg1	concentrations					465:478	high concentrations	460:478	high concentrations of oligosaccharides, particularly melezitose	460:523	This crystallization has been ascribed to high concentrations of oligosaccharides, particularly melezitose.
34420756	0	0	theme	graphene	94:101	arg1	film					119:122	graphene oxide composite film	94:122	graphene oxide composite film	94:122	Green and cost-effective synthesis of flexible, highly conductive cellulose nanofiber/reduced graphene oxide composite film with deep eutectic solvent.
34420756	2	1	theme	choline	469:475	arg1	chloride					477:484	choline chloride	469:484	choline chloride	469:484	In this study, an instant synthesis method for cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES) based on choline chloride and urea as a media is developed.
34420756	4	2	theme	electrochemical	892:906	arg1	properties					908:917	electrochemical properties	892:917	electrochemical properties	892:917	As-obtained films feature excellent flexibility, high electrical conductivity (as high as 26.47 S∙cm-1) and well electrochemical properties.
34420756	3	3	theme	neutral	649:655	arg1	systems					638:644	reaction systems	629:644	reaction systems of neutral to alkaline which in favor of electrostatic repulsion arising from deprotonated carboxyl groups at the composite surface	629:776	This DES shows favorable abilities of recyclability, materials dispersion, and could adjust the pH value for reaction systems of neutral to alkaline which in favor of electrostatic repulsion arising from deprotonated carboxyl groups at the composite surface.
34420756	5	4	theme	mild	1020:1023	arg1	condition					1025:1033	a mild condition	1018:1033	a mild condition	1018:1033	Furthermore, a little amount of nitrogen atoms (~3.0 at%) could be introduced in the composite at a mild condition.
34420756	1	5	theme	energy	284:289	arg1	devices					299:305	energy storage devices	284:305	energy storage devices	284:305	Developing efficient strategy for nanomaterials dispersion is the key for promoting the utilization of cellulose-based composite in energy storage devices.
34420756	0	6	theme	composite	109:117	arg1	film					119:122	graphene oxide composite film	94:122	graphene oxide composite film	94:122	Green and cost-effective synthesis of flexible, highly conductive cellulose nanofiber/reduced graphene oxide composite film with deep eutectic solvent.
34420756	6	7	theme	numerous	1185:1192	arg1	applications					1203:1214	numerous advanced applications	1185:1214	numerous advanced applications	1185:1214	Overall, this approach offers the potential for cost-effective, environmentally friendly and large-scale production of cellulose-based electrode and numerous advanced applications.
34420756	6	8	theme	cellulose-based	1155:1169	arg1	electrode					1171:1179	cellulose-based electrode	1155:1179	cellulose-based electrode	1155:1179	Overall, this approach offers the potential for cost-effective, environmentally friendly and large-scale production of cellulose-based electrode and numerous advanced applications.
34420756	1	9	theme	storage	291:297	arg1	devices					299:305	energy storage devices	284:305	energy storage devices	284:305	Developing efficient strategy for nanomaterials dispersion is the key for promoting the utilization of cellulose-based composite in energy storage devices.
34420756	0	10	theme	oxide	103:107	arg1	film					119:122	graphene oxide composite film	94:122	graphene oxide composite film	94:122	Green and cost-effective synthesis of flexible, highly conductive cellulose nanofiber/reduced graphene oxide composite film with deep eutectic solvent.
34420756	5	11	theme	nitrogen	952:959	arg1	%					975:975	~3.0 at%	968:975	~3.0 at%	968:975	Furthermore, a little amount of nitrogen atoms (~3.0 at%) could be introduced in the composite at a mild condition.
34420756	5	11	theme	nitrogen	952:959	arg1	atoms					961:965	nitrogen atoms	952:965	nitrogen atoms (~3.0 at%)	952:976	Furthermore, a little amount of nitrogen atoms (~3.0 at%) could be introduced in the composite at a mild condition.
34420756	5	12	from	%	975:975	arg1	at					973:974	~3.0 at%	968:975	~3.0 at%	968:975	Furthermore, a little amount of nitrogen atoms (~3.0 at%) could be introduced in the composite at a mild condition.
34420756	3	13	theme	electrostatic	687:699	arg1	repulsion					701:709	electrostatic repulsion	687:709	electrostatic repulsion arising from deprotonated carboxyl groups at the composite surface	687:776	This DES shows favorable abilities of recyclability, materials dispersion, and could adjust the pH value for reaction systems of neutral to alkaline which in favor of electrostatic repulsion arising from deprotonated carboxyl groups at the composite surface.
34420756	4	14	theme	high	828:831	arg1	conductivity					844:855	high electrical conductivity	828:855	high electrical conductivity (as high as 26.47 S∙cm-1)	828:881	As-obtained films feature excellent flexibility, high electrical conductivity (as high as 26.47 S∙cm-1) and well electrochemical properties.
34420756	4	14	theme	high	828:831	arg1	flexibility					815:825	excellent flexibility	805:825	excellent flexibility	805:825	As-obtained films feature excellent flexibility, high electrical conductivity (as high as 26.47 S∙cm-1) and well electrochemical properties.
34420756	5	15	theme	atoms	961:965	arg1	%					975:975	~3.0 at%	968:975	~3.0 at%	968:975	Furthermore, a little amount of nitrogen atoms (~3.0 at%) could be introduced in the composite at a mild condition.
34420756	5	15	theme	atoms	961:965	arg1	amount					942:947	a little amount	933:947	a little amount of nitrogen atoms (~3.0 at%)	933:976	Furthermore, a little amount of nitrogen atoms (~3.0 at%) could be introduced in the composite at a mild condition.
34420756	5	15	theme	atoms	961:965	arg1	atoms					961:965	nitrogen atoms	952:965	nitrogen atoms (~3.0 at%)	952:976	Furthermore, a little amount of nitrogen atoms (~3.0 at%) could be introduced in the composite at a mild condition.
34420756	1	16	theme	nanomaterials	186:198	arg1	dispersion					200:209	nanomaterials dispersion	186:209	nanomaterials dispersion	186:209	Developing efficient strategy for nanomaterials dispersion is the key for promoting the utilization of cellulose-based composite in energy storage devices.
34420756	2	17	theme	eutectic	437:444	arg1	DES					455:457	DES	455:457	DES	455:457	In this study, an instant synthesis method for cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES) based on choline chloride and urea as a media is developed.
34420756	2	17	theme	eutectic	437:444	arg1	solvent					446:452	a deep eutectic solvent	430:452	a deep eutectic solvent (DES)	430:458	In this study, an instant synthesis method for cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES) based on choline chloride and urea as a media is developed.
34420756	4	18	dep	conductivity	844:855	arg1	high					861:864	high	861:864	high	861:864	As-obtained films feature excellent flexibility, high electrical conductivity (as high as 26.47 S∙cm-1) and well electrochemical properties.
34420756	2	19	with	film	420:423	arg1	DES					455:457	DES	455:457	DES	455:457	In this study, an instant synthesis method for cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES) based on choline chloride and urea as a media is developed.
34420756	2	19	with	film	420:423	arg1	solvent					446:452	a deep eutectic solvent	430:452	a deep eutectic solvent (DES)	430:458	In this study, an instant synthesis method for cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES) based on choline chloride and urea as a media is developed.
34420756	2	20	theme	synthesis	334:342	arg1	method					344:349	an instant synthesis method	323:349	an instant synthesis method for cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES) based on choline chloride and urea as a media	323:504	In this study, an instant synthesis method for cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES) based on choline chloride and urea as a media is developed.
34420756	2	21	theme	oxide	398:402	arg1	film					420:423	cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film	355:423	cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES)	355:458	In this study, an instant synthesis method for cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES) based on choline chloride and urea as a media is developed.
34420756	0	22	theme	Green	0:4	arg1	synthesis					25:33	Green and cost-effective synthesis	0:33	Green and cost-effective synthesis of flexible, highly conductive cellulose	0:74	Green and cost-effective synthesis of flexible, highly conductive cellulose nanofiber/reduced graphene oxide composite film with deep eutectic solvent.
34420756	4	23	theme	electrical	833:842	arg1	conductivity					844:855	high electrical conductivity	828:855	high electrical conductivity (as high as 26.47 S∙cm-1)	828:881	As-obtained films feature excellent flexibility, high electrical conductivity (as high as 26.47 S∙cm-1) and well electrochemical properties.
34420756	4	23	theme	electrical	833:842	arg1	flexibility					815:825	excellent flexibility	805:825	excellent flexibility	805:825	As-obtained films feature excellent flexibility, high electrical conductivity (as high as 26.47 S∙cm-1) and well electrochemical properties.
34420756	2	24	theme	instant	326:332	arg1	method					344:349	an instant synthesis method	323:349	an instant synthesis method for cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES) based on choline chloride and urea as a media	323:504	In this study, an instant synthesis method for cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES) based on choline chloride and urea as a media is developed.
34420756	2	25	theme	graphene	389:396	arg1	film					420:423	cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film	355:423	cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES)	355:458	In this study, an instant synthesis method for cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES) based on choline chloride and urea as a media is developed.
34420756	2	26	theme	deep	432:435	arg1	DES					455:457	DES	455:457	DES	455:457	In this study, an instant synthesis method for cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES) based on choline chloride and urea as a media is developed.
34420756	2	26	theme	deep	432:435	arg1	solvent					446:452	a deep eutectic solvent	430:452	a deep eutectic solvent (DES)	430:458	In this study, an instant synthesis method for cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES) based on choline chloride and urea as a media is developed.
34420756	3	27	theme	deprotonated	724:735	arg1	groups					746:751	deprotonated carboxyl groups	724:751	deprotonated carboxyl groups at the composite surface	724:776	This DES shows favorable abilities of recyclability, materials dispersion, and could adjust the pH value for reaction systems of neutral to alkaline which in favor of electrostatic repulsion arising from deprotonated carboxyl groups at the composite surface.
34420756	0	28	theme	eutectic	134:141	arg1	solvent					143:149	deep eutectic solvent	129:149	deep eutectic solvent	129:149	Green and cost-effective synthesis of flexible, highly conductive cellulose nanofiber/reduced graphene oxide composite film with deep eutectic solvent.
34420756	4	29	theme	excellent	805:813	arg1	conductivity					844:855	high electrical conductivity	828:855	high electrical conductivity (as high as 26.47 S∙cm-1)	828:881	As-obtained films feature excellent flexibility, high electrical conductivity (as high as 26.47 S∙cm-1) and well electrochemical properties.
34420756	4	29	theme	excellent	805:813	arg1	flexibility					815:825	excellent flexibility	805:825	excellent flexibility	805:825	As-obtained films feature excellent flexibility, high electrical conductivity (as high as 26.47 S∙cm-1) and well electrochemical properties.
34420756	5	30	theme	little	935:940	arg1	%					975:975	~3.0 at%	968:975	~3.0 at%	968:975	Furthermore, a little amount of nitrogen atoms (~3.0 at%) could be introduced in the composite at a mild condition.
34420756	5	30	theme	little	935:940	arg1	amount					942:947	a little amount	933:947	a little amount of nitrogen atoms (~3.0 at%)	933:976	Furthermore, a little amount of nitrogen atoms (~3.0 at%) could be introduced in the composite at a mild condition.
34420756	5	30	theme	little	935:940	arg1	atoms					961:965	nitrogen atoms	952:965	nitrogen atoms (~3.0 at%)	952:976	Furthermore, a little amount of nitrogen atoms (~3.0 at%) could be introduced in the composite at a mild condition.
34420756	0	31	theme	cost-effective	10:23	arg1	synthesis					25:33	Green and cost-effective synthesis	0:33	Green and cost-effective synthesis of flexible, highly conductive cellulose	0:74	Green and cost-effective synthesis of flexible, highly conductive cellulose nanofiber/reduced graphene oxide composite film with deep eutectic solvent.
34420756	3	32	dep	alkaline	660:667	arg1	which					669:673	which	669:673	which	669:673	This DES shows favorable abilities of recyclability, materials dispersion, and could adjust the pH value for reaction systems of neutral to alkaline which in favor of electrostatic repulsion arising from deprotonated carboxyl groups at the composite surface.
34420756	0	33	theme	deep	129:132	arg1	solvent					143:149	deep eutectic solvent	129:149	deep eutectic solvent	129:149	Green and cost-effective synthesis of flexible, highly conductive cellulose nanofiber/reduced graphene oxide composite film with deep eutectic solvent.
34420756	3	34	theme	pH	616:617	arg1	value					619:623	the pH value	612:623	the pH value for reaction systems of neutral to alkaline which in favor of electrostatic repulsion arising from deprotonated carboxyl groups at the composite surface	612:776	This DES shows favorable abilities of recyclability, materials dispersion, and could adjust the pH value for reaction systems of neutral to alkaline which in favor of electrostatic repulsion arising from deprotonated carboxyl groups at the composite surface.
34420756	2	35	theme	/reduced	380:387	arg1	film					420:423	cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film	355:423	cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES)	355:458	In this study, an instant synthesis method for cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES) based on choline chloride and urea as a media is developed.
34420756	0	36	theme	flexible	38:45	arg1	cellulose					66:74	flexible, highly conductive cellulose	38:74	flexible, highly conductive cellulose	38:74	Green and cost-effective synthesis of flexible, highly conductive cellulose nanofiber/reduced graphene oxide composite film with deep eutectic solvent.
34420756	6	37	theme	electrode	1171:1179	arg1	potential					1070:1078	the potential	1066:1078	the potential for cost-effective, environmentally friendly	1066:1123	Overall, this approach offers the potential for cost-effective, environmentally friendly and large-scale production of cellulose-based electrode and numerous advanced applications.
34420756	6	37	theme	electrode	1171:1179	arg1	production					1141:1150	large-scale production	1129:1150	large-scale production of cellulose-based electrode and numerous advanced applications	1129:1214	Overall, this approach offers the potential for cost-effective, environmentally friendly and large-scale production of cellulose-based electrode and numerous advanced applications.
34420756	2	38	theme	composite	410:418	arg1	film					420:423	cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film	355:423	cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES)	355:458	In this study, an instant synthesis method for cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES) based on choline chloride and urea as a media is developed.
34420756	2	39	theme	rGO	405:407	arg1	film					420:423	cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film	355:423	cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES)	355:458	In this study, an instant synthesis method for cellulose nanofiber (CNF)/reduced graphene oxide (rGO) composite film with a deep eutectic solvent (DES) based on choline chloride and urea as a media is developed.
34420756	3	40	theme	composite	760:768	arg1	surface					770:776	the composite surface	756:776	the composite surface	756:776	This DES shows favorable abilities of recyclability, materials dispersion, and could adjust the pH value for reaction systems of neutral to alkaline which in favor of electrostatic repulsion arising from deprotonated carboxyl groups at the composite surface.
34420756	3	41	from	alkaline	660:667	arg1	favor					678:682	favor	678:682	favor	678:682	This DES shows favorable abilities of recyclability, materials dispersion, and could adjust the pH value for reaction systems of neutral to alkaline which in favor of electrostatic repulsion arising from deprotonated carboxyl groups at the composite surface.
34420756	0	42	dep	flexible	38:45	arg1	conductive					55:64	conductive	55:64	conductive	55:64	Green and cost-effective synthesis of flexible, highly conductive cellulose nanofiber/reduced graphene oxide composite film with deep eutectic solvent.
34420756	6	43	theme	cost-effective	1084:1097	arg1	friendly					1116:1123	cost-effective, environmentally friendly	1084:1123	cost-effective, environmentally friendly	1084:1123	Overall, this approach offers the potential for cost-effective, environmentally friendly and large-scale production of cellulose-based electrode and numerous advanced applications.
34420756	3	44	theme	carboxyl	737:744	arg1	groups					746:751	deprotonated carboxyl groups	724:751	deprotonated carboxyl groups at the composite surface	724:776	This DES shows favorable abilities of recyclability, materials dispersion, and could adjust the pH value for reaction systems of neutral to alkaline which in favor of electrostatic repulsion arising from deprotonated carboxyl groups at the composite surface.
34420756	3	45	from	surface	770:776	arg1	groups					746:751	deprotonated carboxyl groups	724:751	deprotonated carboxyl groups at the composite surface	724:776	This DES shows favorable abilities of recyclability, materials dispersion, and could adjust the pH value for reaction systems of neutral to alkaline which in favor of electrostatic repulsion arising from deprotonated carboxyl groups at the composite surface.
34420756	3	46	theme	recyclability	558:570	arg1	abilities					545:553	favorable abilities	535:553	favorable abilities of recyclability	535:570	This DES shows favorable abilities of recyclability, materials dispersion, and could adjust the pH value for reaction systems of neutral to alkaline which in favor of electrostatic repulsion arising from deprotonated carboxyl groups at the composite surface.
34420756	6	47	dep	cost-effective	1084:1097	arg1	environmentally					1100:1114	environmentally	1100:1114	environmentally	1100:1114	Overall, this approach offers the potential for cost-effective, environmentally friendly and large-scale production of cellulose-based electrode and numerous advanced applications.
34420756	6	48	theme	applications	1203:1214	arg1	potential					1070:1078	the potential	1066:1078	the potential for cost-effective, environmentally friendly	1066:1123	Overall, this approach offers the potential for cost-effective, environmentally friendly and large-scale production of cellulose-based electrode and numerous advanced applications.
34420756	6	48	theme	applications	1203:1214	arg1	production					1141:1150	large-scale production	1129:1150	large-scale production of cellulose-based electrode and numerous advanced applications	1129:1214	Overall, this approach offers the potential for cost-effective, environmentally friendly and large-scale production of cellulose-based electrode and numerous advanced applications.
34420756	3	49	theme	favorable	535:543	arg1	abilities					545:553	favorable abilities	535:553	favorable abilities of recyclability	535:570	This DES shows favorable abilities of recyclability, materials dispersion, and could adjust the pH value for reaction systems of neutral to alkaline which in favor of electrostatic repulsion arising from deprotonated carboxyl groups at the composite surface.
34420756	1	50	from	utilization	240:250	arg1	devices					299:305	energy storage devices	284:305	energy storage devices	284:305	Developing efficient strategy for nanomaterials dispersion is the key for promoting the utilization of cellulose-based composite in energy storage devices.
34420756	6	51	theme	advanced	1194:1201	arg1	applications					1203:1214	numerous advanced applications	1185:1214	numerous advanced applications	1185:1214	Overall, this approach offers the potential for cost-effective, environmentally friendly and large-scale production of cellulose-based electrode and numerous advanced applications.
34420756	6	52	theme	large-scale	1129:1139	arg1	production					1141:1150	large-scale production	1129:1150	large-scale production of cellulose-based electrode and numerous advanced applications	1129:1214	Overall, this approach offers the potential for cost-effective, environmentally friendly and large-scale production of cellulose-based electrode and numerous advanced applications.
34420756	5	53	theme	at	973:974	arg1	%					975:975	~3.0 at%	968:975	~3.0 at%	968:975	Furthermore, a little amount of nitrogen atoms (~3.0 at%) could be introduced in the composite at a mild condition.
34420756	5	53	theme	at	973:974	arg1	atoms					961:965	nitrogen atoms	952:965	nitrogen atoms (~3.0 at%)	952:976	Furthermore, a little amount of nitrogen atoms (~3.0 at%) could be introduced in the composite at a mild condition.
34420756	0	54	theme	cellulose	66:74	arg1	synthesis					25:33	Green and cost-effective synthesis	0:33	Green and cost-effective synthesis of flexible, highly conductive cellulose	0:74	Green and cost-effective synthesis of flexible, highly conductive cellulose nanofiber/reduced graphene oxide composite film with deep eutectic solvent.
34420756	3	55	theme	reaction	629:636	arg1	systems					638:644	reaction systems	629:644	reaction systems of neutral to alkaline which in favor of electrostatic repulsion arising from deprotonated carboxyl groups at the composite surface	629:776	This DES shows favorable abilities of recyclability, materials dispersion, and could adjust the pH value for reaction systems of neutral to alkaline which in favor of electrostatic repulsion arising from deprotonated carboxyl groups at the composite surface.
34420756	4	56	theme	As-obtained	779:789	arg1	films					791:795	As-obtained films	779:795	As-obtained films	779:795	As-obtained films feature excellent flexibility, high electrical conductivity (as high as 26.47 S∙cm-1) and well electrochemical properties.
34420756	1	57	theme	Developing	152:161	arg1	strategy					173:180	Developing efficient strategy	152:180	Developing efficient strategy for nanomaterials dispersion	152:209	Developing efficient strategy for nanomaterials dispersion is the key for promoting the utilization of cellulose-based composite in energy storage devices.
34420756	1	57	theme	Developing	152:161	arg1	key					218:220	key	218:220	key	218:220	Developing efficient strategy for nanomaterials dispersion is the key for promoting the utilization of cellulose-based composite in energy storage devices.
34420756	1	58	theme	cellulose-based	255:269	arg1	composite					271:279	cellulose-based composite	255:279	cellulose-based composite	255:279	Developing efficient strategy for nanomaterials dispersion is the key for promoting the utilization of cellulose-based composite in energy storage devices.
34420756	5	59	dep	condition	1025:1033	arg1	the					1001:1003	the	1001:1003	the	1001:1003	Furthermore, a little amount of nitrogen atoms (~3.0 at%) could be introduced in the composite at a mild condition.
34420756	5	59	dep	condition	1025:1033	arg1	composite					1005:1013	composite	1005:1013	composite	1005:1013	Furthermore, a little amount of nitrogen atoms (~3.0 at%) could be introduced in the composite at a mild condition.
34420756	1	60	theme	efficient	163:171	arg1	strategy					173:180	Developing efficient strategy	152:180	Developing efficient strategy for nanomaterials dispersion	152:209	Developing efficient strategy for nanomaterials dispersion is the key for promoting the utilization of cellulose-based composite in energy storage devices.
34420756	1	60	theme	efficient	163:171	arg1	key					218:220	key	218:220	key	218:220	Developing efficient strategy for nanomaterials dispersion is the key for promoting the utilization of cellulose-based composite in energy storage devices.
34420756	1	61	theme	composite	271:279	arg1	utilization					240:250	the utilization	236:250	the utilization of cellulose-based composite in energy storage devices	236:305	Developing efficient strategy for nanomaterials dispersion is the key for promoting the utilization of cellulose-based composite in energy storage devices.
33254969	9	0	theme	dextran	1614:1620	arg1	expansion					1622:1630	the induced dextran expansion	1602:1630	the induced dextran expansion	1602:1630	Under electrical potentials, the corresponding diffusion coefficients were much higher due mainly to the electro-repulsive force between the negatively charged electrode and the negatively charged dextran and the induced dextran expansion.
33254969	7	1	theme	release	1148:1154	arg1	mechanism					1156:1164	the release mechanism	1144:1164	the release mechanism	1144:1164	Under applied electrical potentials, the release mechanism was a combination between the Fickian diffusion and the matrix swelling.
33254969	7	1	theme	release	1148:1154	arg1	combination					1172:1182	a combination	1170:1182	a combination between the Fickian diffusion and the matrix swelling	1170:1236	Under applied electrical potentials, the release mechanism was a combination between the Fickian diffusion and the matrix swelling.
33254969	8	2	from	hydrogels	1287:1295	arg1	coefficients					1257:1268	The Dcf diffusion coefficients	1239:1268	The Dcf diffusion coefficients from the dextran hydrogels without electrical potential	1239:1324	The Dcf diffusion coefficients from the dextran hydrogels without electrical potential increased with decreasing crosslinking ratio and molecular weight.
33254969	5	3	from	hydrogels	653:661	arg1	release					629:635	The in-vitro release	616:635	The in-vitro release of Dcf from the hydrogels	616:661	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	6	4	theme	Fickian	1088:1094	arg1	diffusion					1096:1104	the Fickian diffusion	1084:1104	the Fickian diffusion	1084:1104	The release mechanism of Dcf from the dextran hydrogels and the composite without electrical potential was the diffusion controlled mechanism or the Fickian diffusion.
33254969	2	5	used	used	273:276	arg2	hydrogels					290:298	hydrogels	290:298	hydrogels	290:298	The matrix materials that have been widely used in TDDS are hydrogels.
33254969	2	5	used	used	273:276	arg2	materials					241:249	The matrix materials	230:249	The matrix materials that have been widely used in TDDS	230:284	The matrix materials that have been widely used in TDDS are hydrogels.
33254969	10	6	theme	release	1915:1921	arg1	enhancer					1923:1930	a drug release enhancer	1908:1930	a drug release enhancer under electric field through the reduction reaction	1908:1982	For the Dcf-loaded PEAn/dextran composite, the diffusion coefficient was enhanced by two orders of magnitude when the electric potential was applied, specifically illustrating the unique features of PEAn as an efficient drug encapsulation host without electric field, and as a drug release enhancer under electric field through the reduction reaction.
33254969	10	7	theme	magnitude	1732:1740	arg1	orders					1722:1727	two orders	1718:1727	two orders of magnitude	1718:1740	For the Dcf-loaded PEAn/dextran composite, the diffusion coefficient was enhanced by two orders of magnitude when the electric potential was applied, specifically illustrating the unique features of PEAn as an efficient drug encapsulation host without electric field, and as a drug release enhancer under electric field through the reduction reaction.
33254969	1	8	used	used	149:152	arg2	systems					130:136	Transdermal drug delivery systems	104:136	Transdermal drug delivery systems (TDDS)	104:143	Transdermal drug delivery systems (TDDS) are used as an alternative route to deliver drugs into the blood system for therapy.
33254969	1	8	used	used	149:152	arg2	route					172:176	an alternative route	157:176	an alternative route to deliver drugs into the blood system for therapy	157:227	Transdermal drug delivery systems (TDDS) are used as an alternative route to deliver drugs into the blood system for therapy.
33254969	1	8	used	used	149:152	arg2	TDDS					139:142	TDDS	139:142	TDDS	139:142	Transdermal drug delivery systems (TDDS) are used as an alternative route to deliver drugs into the blood system for therapy.
33254969	4	9	theme	encapsulation	596:608	arg1	host					610:613	the drug encapsulation host	587:613	the drug encapsulation host	587:613	Poly(2-ethylaniline) (PEAn) was successfully synthesized and embedded into the dextran hydrogel as the drug encapsulation host.
33254969	6	10	theme	controlled	1060:1069	arg1	mechanism					951:959	The release mechanism	939:959	The release mechanism of Dcf from the dextran hydrogels and the composite without electrical potential	939:1040	The release mechanism of Dcf from the dextran hydrogels and the composite without electrical potential was the diffusion controlled mechanism or the Fickian diffusion.
33254969	6	10	theme	controlled	1060:1069	arg1	mechanism					1071:1079	the diffusion controlled mechanism	1046:1079	the diffusion controlled mechanism	1046:1079	The release mechanism of Dcf from the dextran hydrogels and the composite without electrical potential was the diffusion controlled mechanism or the Fickian diffusion.
33254969	9	11	theme	electrical	1399:1408	arg1	potentials					1410:1419	electrical potentials	1399:1419	electrical potentials	1399:1419	Under electrical potentials, the corresponding diffusion coefficients were much higher due mainly to the electro-repulsive force between the negatively charged electrode and the negatively charged dextran and the induced dextran expansion.
33254969	5	12	theme	crosslinking	837:848	arg1	ratios					850:855	crosslinking ratios	837:855	crosslinking ratios	837:855	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	9	13	theme	corresponding	1426:1438	arg1	coefficients					1450:1461	the corresponding diffusion coefficients	1422:1461	the corresponding diffusion coefficients	1422:1461	Under electrical potentials, the corresponding diffusion coefficients were much higher due mainly to the electro-repulsive force between the negatively charged electrode and the negatively charged dextran and the induced dextran expansion.
33254969	9	13	theme	corresponding	1426:1438	arg1	higher					1473:1478	higher	1473:1478	higher	1473:1478	Under electrical potentials, the corresponding diffusion coefficients were much higher due mainly to the electro-repulsive force between the negatively charged electrode and the negatively charged dextran and the induced dextran expansion.
33254969	5	14	theme	in-vitro	620:627	arg1	release					629:635	The in-vitro release	616:635	The in-vitro release of Dcf from the hydrogels	616:661	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	5	15	from	cell	713:716	arg1	saline					742:747	a phosphate-buffered saline	721:747	a phosphate-buffered saline (PBS)	721:753	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	5	15	from	cell	713:716	arg1	PBS					750:752	PBS	750:752	PBS	750:752	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	5	16	theme	ratios	850:855	arg1	effects					826:832	the effects	822:832	the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn	822:936	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	5	17	from	37 °C	788:792	arg1	solution					755:762	a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution	686:762	a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h	686:813	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	3	18	theme	dextran	305:311	arg1	hydrogels					313:321	The dextran hydrogels	301:321	The dextran hydrogels	301:321	The dextran hydrogels were prepared by the solution casting using trisodium trimetaphosphate (STMP) as the crosslinking agent, and diclofenac sodium salt (Dcf) as the anionic model drug.
33254969	5	19	theme	Franz-Diffusion	697:711	arg1	cell					713:716	Franz-Diffusion cell	697:716	a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h	686:813	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	8	20	theme	Dcf	1243:1245	arg1	coefficients					1257:1268	The Dcf diffusion coefficients	1239:1268	The Dcf diffusion coefficients from the dextran hydrogels without electrical potential	1239:1324	The Dcf diffusion coefficients from the dextran hydrogels without electrical potential increased with decreasing crosslinking ratio and molecular weight.
33254969	0	21	theme	diclofenac	84:93	arg1	release					95:101	electrically controlled diclofenac release	60:101	electrically controlled diclofenac release	60:101	Conductive poly(2-ethylaniline) dextran-based hydrogels for electrically controlled diclofenac release.
33254969	5	22	theme	24 h	810:813	arg1	period					800:805	a period	798:805	a period of 24 h	798:813	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	7	23	theme	applied	1113:1119	arg1	potentials					1132:1141	applied electrical potentials	1113:1141	applied electrical potentials	1113:1141	Under applied electrical potentials, the release mechanism was a combination between the Fickian diffusion and the matrix swelling.
33254969	5	24	theme	molecular	866:874	arg1	weights					876:882	dextran molecular weights	858:882	dextran molecular weights	858:882	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	7	25	theme	matrix	1222:1227	arg1	swelling					1229:1236	the matrix swelling	1218:1236	the matrix swelling	1218:1236	Under applied electrical potentials, the release mechanism was a combination between the Fickian diffusion and the matrix swelling.
33254969	3	26	theme	crosslinking	408:419	arg1	agent					421:425	the crosslinking agent	404:425	the crosslinking agent	404:425	The dextran hydrogels were prepared by the solution casting using trisodium trimetaphosphate (STMP) as the crosslinking agent, and diclofenac sodium salt (Dcf) as the anionic model drug.
33254969	3	26	theme	crosslinking	408:419	arg1	trimetaphosphate					377:392	trisodium trimetaphosphate	367:392	trisodium trimetaphosphate (STMP)	367:399	The dextran hydrogels were prepared by the solution casting using trisodium trimetaphosphate (STMP) as the crosslinking agent, and diclofenac sodium salt (Dcf) as the anionic model drug.
33254969	5	27	theme	modified	688:695	arg1	solution					755:762	a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution	686:762	a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h	686:813	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	0	28	theme	Conductive	0:9	arg1	2-ethylaniline					16:29	2-ethylaniline	16:29	2-ethylaniline	16:29	Conductive poly(2-ethylaniline) dextran-based hydrogels for electrically controlled diclofenac release.
33254969	0	28	theme	Conductive	0:9	arg1	poly					11:14	Conductive poly	0:14	Conductive poly(2-ethylaniline)	0:30	Conductive poly(2-ethylaniline) dextran-based hydrogels for electrically controlled diclofenac release.
33254969	1	29	theme	Transdermal	104:114	arg1	TDDS					139:142	TDDS	139:142	TDDS	139:142	Transdermal drug delivery systems (TDDS) are used as an alternative route to deliver drugs into the blood system for therapy.
33254969	1	29	theme	Transdermal	104:114	arg1	systems					130:136	Transdermal drug delivery systems	104:136	Transdermal drug delivery systems (TDDS)	104:143	Transdermal drug delivery systems (TDDS) are used as an alternative route to deliver drugs into the blood system for therapy.
33254969	1	29	theme	Transdermal	104:114	arg1	route					172:176	an alternative route	157:176	an alternative route to deliver drugs into the blood system for therapy	157:227	Transdermal drug delivery systems (TDDS) are used as an alternative route to deliver drugs into the blood system for therapy.
33254969	3	30	theme	solution	344:351	arg1	casting					353:359	the solution casting	340:359	the solution casting using trisodium trimetaphosphate (STMP) as the crosslinking agent, and diclofenac sodium salt (Dcf) as the anionic model drug	340:485	The dextran hydrogels were prepared by the solution casting using trisodium trimetaphosphate (STMP) as the crosslinking agent, and diclofenac sodium salt (Dcf) as the anionic model drug.
33254969	2	31	theme	matrix	234:239	arg1	materials					241:249	The matrix materials	230:249	The matrix materials that have been widely used in TDDS	230:284	The matrix materials that have been widely used in TDDS are hydrogels.
33254969	2	31	theme	matrix	234:239	arg1	hydrogels					290:298	hydrogels	290:298	hydrogels	290:298	The matrix materials that have been widely used in TDDS are hydrogels.
33254969	8	32	theme	electrical	1305:1314	arg1	potential					1316:1324	electrical potential	1305:1324	electrical potential	1305:1324	The Dcf diffusion coefficients from the dextran hydrogels without electrical potential increased with decreasing crosslinking ratio and molecular weight.
33254969	3	33	theme	diclofenac	432:441	arg1	Dcf					456:458	Dcf	456:458	Dcf	456:458	The dextran hydrogels were prepared by the solution casting using trisodium trimetaphosphate (STMP) as the crosslinking agent, and diclofenac sodium salt (Dcf) as the anionic model drug.
33254969	3	33	theme	diclofenac	432:441	arg1	salt					450:453	diclofenac sodium salt	432:453	diclofenac sodium salt (Dcf)	432:459	The dextran hydrogels were prepared by the solution casting using trisodium trimetaphosphate (STMP) as the crosslinking agent, and diclofenac sodium salt (Dcf) as the anionic model drug.
33254969	1	34	theme	delivery	121:128	arg1	TDDS					139:142	TDDS	139:142	TDDS	139:142	Transdermal drug delivery systems (TDDS) are used as an alternative route to deliver drugs into the blood system for therapy.
33254969	1	34	theme	delivery	121:128	arg1	systems					130:136	Transdermal drug delivery systems	104:136	Transdermal drug delivery systems (TDDS)	104:143	Transdermal drug delivery systems (TDDS) are used as an alternative route to deliver drugs into the blood system for therapy.
33254969	1	34	theme	delivery	121:128	arg1	route					172:176	an alternative route	157:176	an alternative route to deliver drugs into the blood system for therapy	157:227	Transdermal drug delivery systems (TDDS) are used as an alternative route to deliver drugs into the blood system for therapy.
33254969	6	35	theme	release	943:949	arg1	mechanism					951:959	The release mechanism	939:959	The release mechanism of Dcf from the dextran hydrogels and the composite without electrical potential	939:1040	The release mechanism of Dcf from the dextran hydrogels and the composite without electrical potential was the diffusion controlled mechanism or the Fickian diffusion.
33254969	6	35	theme	release	943:949	arg1	mechanism					1071:1079	the diffusion controlled mechanism	1046:1079	the diffusion controlled mechanism	1046:1079	The release mechanism of Dcf from the dextran hydrogels and the composite without electrical potential was the diffusion controlled mechanism or the Fickian diffusion.
33254969	10	36	theme	Dcf-loaded	1641:1650	arg1	composite					1665:1673	the Dcf-loaded PEAn/dextran composite	1637:1673	the Dcf-loaded PEAn/dextran composite	1637:1673	For the Dcf-loaded PEAn/dextran composite, the diffusion coefficient was enhanced by two orders of magnitude when the electric potential was applied, specifically illustrating the unique features of PEAn as an efficient drug encapsulation host without electric field, and as a drug release enhancer under electric field through the reduction reaction.
33254969	5	37	theme	phosphate-buffered	723:740	arg1	saline					742:747	a phosphate-buffered saline	721:747	a phosphate-buffered saline (PBS)	721:753	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	5	37	theme	phosphate-buffered	723:740	arg1	PBS					750:752	PBS	750:752	PBS	750:752	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	5	38	theme	conductive	914:923	arg1	PEAn					933:936	the conductive polymer PEAn	910:936	the conductive polymer PEAn	910:936	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	10	39	theme	PEAn	1832:1835	arg1	host					1872:1875	an efficient drug encapsulation host	1840:1875	an efficient drug encapsulation host without electric field	1840:1898	For the Dcf-loaded PEAn/dextran composite, the diffusion coefficient was enhanced by two orders of magnitude when the electric potential was applied, specifically illustrating the unique features of PEAn as an efficient drug encapsulation host without electric field, and as a drug release enhancer under electric field through the reduction reaction.
33254969	10	39	theme	PEAn	1832:1835	arg1	features					1820:1827	the unique features	1809:1827	the unique features of PEAn	1809:1835	For the Dcf-loaded PEAn/dextran composite, the diffusion coefficient was enhanced by two orders of magnitude when the electric potential was applied, specifically illustrating the unique features of PEAn as an efficient drug encapsulation host without electric field, and as a drug release enhancer under electric field through the reduction reaction.
33254969	6	40	from	composite	1003:1011	arg1	mechanism					951:959	The release mechanism	939:959	The release mechanism of Dcf from the dextran hydrogels and the composite without electrical potential	939:1040	The release mechanism of Dcf from the dextran hydrogels and the composite without electrical potential was the diffusion controlled mechanism or the Fickian diffusion.
33254969	6	40	from	composite	1003:1011	arg1	mechanism					1071:1079	the diffusion controlled mechanism	1046:1079	the diffusion controlled mechanism	1046:1079	The release mechanism of Dcf from the dextran hydrogels and the composite without electrical potential was the diffusion controlled mechanism or the Fickian diffusion.
33254969	9	41	theme	charged	1582:1588	arg1	dextran					1590:1596	the negatively charged dextran	1567:1596	the negatively charged dextran	1567:1596	Under electrical potentials, the corresponding diffusion coefficients were much higher due mainly to the electro-repulsive force between the negatively charged electrode and the negatively charged dextran and the induced dextran expansion.
33254969	7	42	theme	Fickian	1196:1202	arg1	diffusion					1204:1212	the Fickian diffusion	1192:1212	the Fickian diffusion	1192:1212	Under applied electrical potentials, the release mechanism was a combination between the Fickian diffusion and the matrix swelling.
33254969	5	43	theme	potentials	894:903	arg1	effects					826:832	the effects	822:832	the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn	822:936	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	10	44	theme	drug	1853:1856	arg1	features					1820:1827	the unique features	1809:1827	the unique features of PEAn	1809:1835	For the Dcf-loaded PEAn/dextran composite, the diffusion coefficient was enhanced by two orders of magnitude when the electric potential was applied, specifically illustrating the unique features of PEAn as an efficient drug encapsulation host without electric field, and as a drug release enhancer under electric field through the reduction reaction.
33254969	10	44	theme	drug	1853:1856	arg1	host					1872:1875	an efficient drug encapsulation host	1840:1875	an efficient drug encapsulation host without electric field	1840:1898	For the Dcf-loaded PEAn/dextran composite, the diffusion coefficient was enhanced by two orders of magnitude when the electric potential was applied, specifically illustrating the unique features of PEAn as an efficient drug encapsulation host without electric field, and as a drug release enhancer under electric field through the reduction reaction.
33254969	8	45	theme	molecular	1375:1383	arg1	weight					1385:1390	molecular weight	1375:1390	molecular weight	1375:1390	The Dcf diffusion coefficients from the dextran hydrogels without electrical potential increased with decreasing crosslinking ratio and molecular weight.
33254969	3	46	theme	anionic	468:474	arg1	drug					482:485	the anionic model drug	464:485	the anionic model drug	464:485	The dextran hydrogels were prepared by the solution casting using trisodium trimetaphosphate (STMP) as the crosslinking agent, and diclofenac sodium salt (Dcf) as the anionic model drug.
33254969	3	46	theme	anionic	468:474	arg1	trimetaphosphate					377:392	trisodium trimetaphosphate	367:392	trisodium trimetaphosphate (STMP)	367:399	The dextran hydrogels were prepared by the solution casting using trisodium trimetaphosphate (STMP) as the crosslinking agent, and diclofenac sodium salt (Dcf) as the anionic model drug.
33254969	9	47	theme	induced	1606:1612	arg1	expansion					1622:1630	the induced dextran expansion	1602:1630	the induced dextran expansion	1602:1630	Under electrical potentials, the corresponding diffusion coefficients were much higher due mainly to the electro-repulsive force between the negatively charged electrode and the negatively charged dextran and the induced dextran expansion.
33254969	7	48	theme	electrical	1121:1130	arg1	potentials					1132:1141	applied electrical potentials	1113:1141	applied electrical potentials	1113:1141	Under applied electrical potentials, the release mechanism was a combination between the Fickian diffusion and the matrix swelling.
33254969	6	49	theme	dextran	977:983	arg1	hydrogels					985:993	the dextran hydrogels	973:993	the dextran hydrogels	973:993	The release mechanism of Dcf from the dextran hydrogels and the composite without electrical potential was the diffusion controlled mechanism or the Fickian diffusion.
33254969	10	50	theme	electric	1885:1892	arg1	field					1894:1898	electric field	1885:1898	electric field	1885:1898	For the Dcf-loaded PEAn/dextran composite, the diffusion coefficient was enhanced by two orders of magnitude when the electric potential was applied, specifically illustrating the unique features of PEAn as an efficient drug encapsulation host without electric field, and as a drug release enhancer under electric field through the reduction reaction.
33254969	10	51	theme	drug	1910:1913	arg1	enhancer					1923:1930	a drug release enhancer	1908:1930	a drug release enhancer under electric field through the reduction reaction	1908:1982	For the Dcf-loaded PEAn/dextran composite, the diffusion coefficient was enhanced by two orders of magnitude when the electric potential was applied, specifically illustrating the unique features of PEAn as an efficient drug encapsulation host without electric field, and as a drug release enhancer under electric field through the reduction reaction.
33254969	1	52	theme	alternative	160:170	arg1	systems					130:136	Transdermal drug delivery systems	104:136	Transdermal drug delivery systems (TDDS)	104:143	Transdermal drug delivery systems (TDDS) are used as an alternative route to deliver drugs into the blood system for therapy.
33254969	1	52	theme	alternative	160:170	arg1	route					172:176	an alternative route	157:176	an alternative route to deliver drugs into the blood system for therapy	157:227	Transdermal drug delivery systems (TDDS) are used as an alternative route to deliver drugs into the blood system for therapy.
33254969	4	53	theme	drug	591:594	arg1	host					610:613	the drug encapsulation host	587:613	the drug encapsulation host	587:613	Poly(2-ethylaniline) (PEAn) was successfully synthesized and embedded into the dextran hydrogel as the drug encapsulation host.
33254969	10	54	theme	electric	1938:1945	arg1	field					1947:1951	electric field	1938:1951	electric field through the reduction reaction	1938:1982	For the Dcf-loaded PEAn/dextran composite, the diffusion coefficient was enhanced by two orders of magnitude when the electric potential was applied, specifically illustrating the unique features of PEAn as an efficient drug encapsulation host without electric field, and as a drug release enhancer under electric field through the reduction reaction.
33254969	5	55	theme	Dcf	640:642	arg1	release					629:635	The in-vitro release	616:635	The in-vitro release of Dcf from the hydrogels	616:661	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	5	56	theme	PEAn	933:936	arg1	effects					826:832	the effects	822:832	the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn	822:936	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	10	57	theme	electric	1751:1758	arg1	potential					1760:1768	the electric potential	1747:1768	the electric potential	1747:1768	For the Dcf-loaded PEAn/dextran composite, the diffusion coefficient was enhanced by two orders of magnitude when the electric potential was applied, specifically illustrating the unique features of PEAn as an efficient drug encapsulation host without electric field, and as a drug release enhancer under electric field through the reduction reaction.
33254969	4	58	theme	dextran	567:573	arg1	hydrogel					575:582	the dextran hydrogel	563:582	the dextran hydrogel	563:582	Poly(2-ethylaniline) (PEAn) was successfully synthesized and embedded into the dextran hydrogel as the drug encapsulation host.
33254969	6	59	theme	electrical	1021:1030	arg1	potential					1032:1040	electrical potential	1021:1040	electrical potential	1021:1040	The release mechanism of Dcf from the dextran hydrogels and the composite without electrical potential was the diffusion controlled mechanism or the Fickian diffusion.
33254969	10	60	theme	reduction	1965:1973	arg1	reaction					1975:1982	the reduction reaction	1961:1982	the reduction reaction	1961:1982	For the Dcf-loaded PEAn/dextran composite, the diffusion coefficient was enhanced by two orders of magnitude when the electric potential was applied, specifically illustrating the unique features of PEAn as an efficient drug encapsulation host without electric field, and as a drug release enhancer under electric field through the reduction reaction.
33254969	9	61	theme	diffusion	1440:1448	arg1	coefficients					1450:1461	the corresponding diffusion coefficients	1422:1461	the corresponding diffusion coefficients	1422:1461	Under electrical potentials, the corresponding diffusion coefficients were much higher due mainly to the electro-repulsive force between the negatively charged electrode and the negatively charged dextran and the induced dextran expansion.
33254969	9	61	theme	diffusion	1440:1448	arg1	higher					1473:1478	higher	1473:1478	higher	1473:1478	Under electrical potentials, the corresponding diffusion coefficients were much higher due mainly to the electro-repulsive force between the negatively charged electrode and the negatively charged dextran and the induced dextran expansion.
33254969	5	62	from	pH	771:772	arg1	solution					755:762	a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution	686:762	a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h	686:813	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	0	63	theme	controlled	73:82	arg1	release					95:101	electrically controlled diclofenac release	60:101	electrically controlled diclofenac release	60:101	Conductive poly(2-ethylaniline) dextran-based hydrogels for electrically controlled diclofenac release.
33254969	10	64	theme	unique	1813:1818	arg1	host					1872:1875	an efficient drug encapsulation host	1840:1875	an efficient drug encapsulation host without electric field	1840:1898	For the Dcf-loaded PEAn/dextran composite, the diffusion coefficient was enhanced by two orders of magnitude when the electric potential was applied, specifically illustrating the unique features of PEAn as an efficient drug encapsulation host without electric field, and as a drug release enhancer under electric field through the reduction reaction.
33254969	10	64	theme	unique	1813:1818	arg1	features					1820:1827	the unique features	1809:1827	the unique features of PEAn	1809:1835	For the Dcf-loaded PEAn/dextran composite, the diffusion coefficient was enhanced by two orders of magnitude when the electric potential was applied, specifically illustrating the unique features of PEAn as an efficient drug encapsulation host without electric field, and as a drug release enhancer under electric field through the reduction reaction.
33254969	9	65	theme	charged	1545:1551	arg1	electrode					1553:1561	the negatively charged electrode	1530:1561	the negatively charged electrode	1530:1561	Under electrical potentials, the corresponding diffusion coefficients were much higher due mainly to the electro-repulsive force between the negatively charged electrode and the negatively charged dextran and the induced dextran expansion.
33254969	5	66	theme	cell	713:716	arg1	solution					755:762	a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution	686:762	a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h	686:813	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	5	67	theme	dextran	858:864	arg1	weights					876:882	dextran molecular weights	858:882	dextran molecular weights	858:882	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	9	68	theme	electro-repulsive	1498:1514	arg1	force					1516:1520	the electro-repulsive force	1494:1520	the electro-repulsive force between the negatively charged electrode and the negatively charged dextran and the induced dextran expansion	1494:1630	Under electrical potentials, the corresponding diffusion coefficients were much higher due mainly to the electro-repulsive force between the negatively charged electrode and the negatively charged dextran and the induced dextran expansion.
33254969	8	69	theme	diffusion	1247:1255	arg1	coefficients					1257:1268	The Dcf diffusion coefficients	1239:1268	The Dcf diffusion coefficients from the dextran hydrogels without electrical potential	1239:1324	The Dcf diffusion coefficients from the dextran hydrogels without electrical potential increased with decreasing crosslinking ratio and molecular weight.
33254969	5	70	theme	weights	876:882	arg1	effects					826:832	the effects	822:832	the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn	822:936	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	6	71	theme	Dcf	964:966	arg1	mechanism					951:959	The release mechanism	939:959	The release mechanism of Dcf from the dextran hydrogels and the composite without electrical potential	939:1040	The release mechanism of Dcf from the dextran hydrogels and the composite without electrical potential was the diffusion controlled mechanism or the Fickian diffusion.
33254969	6	71	theme	Dcf	964:966	arg1	mechanism					1071:1079	the diffusion controlled mechanism	1046:1079	the diffusion controlled mechanism	1046:1079	The release mechanism of Dcf from the dextran hydrogels and the composite without electrical potential was the diffusion controlled mechanism or the Fickian diffusion.
33254969	3	72	theme	trisodium	367:375	arg1	drug					482:485	the anionic model drug	464:485	the anionic model drug	464:485	The dextran hydrogels were prepared by the solution casting using trisodium trimetaphosphate (STMP) as the crosslinking agent, and diclofenac sodium salt (Dcf) as the anionic model drug.
33254969	3	72	theme	trisodium	367:375	arg1	agent					421:425	the crosslinking agent	404:425	the crosslinking agent	404:425	The dextran hydrogels were prepared by the solution casting using trisodium trimetaphosphate (STMP) as the crosslinking agent, and diclofenac sodium salt (Dcf) as the anionic model drug.
33254969	3	72	theme	trisodium	367:375	arg1	STMP					395:398	STMP	395:398	STMP	395:398	The dextran hydrogels were prepared by the solution casting using trisodium trimetaphosphate (STMP) as the crosslinking agent, and diclofenac sodium salt (Dcf) as the anionic model drug.
33254969	3	72	theme	trisodium	367:375	arg1	trimetaphosphate					377:392	trisodium trimetaphosphate	367:392	trisodium trimetaphosphate (STMP)	367:399	The dextran hydrogels were prepared by the solution casting using trisodium trimetaphosphate (STMP) as the crosslinking agent, and diclofenac sodium salt (Dcf) as the anionic model drug.
33254969	8	73	theme	dextran	1279:1285	arg1	hydrogels					1287:1295	the dextran hydrogels	1275:1295	the dextran hydrogels	1275:1295	The Dcf diffusion coefficients from the dextran hydrogels without electrical potential increased with decreasing crosslinking ratio and molecular weight.
33254969	1	74	theme	drug	116:119	arg1	TDDS					139:142	TDDS	139:142	TDDS	139:142	Transdermal drug delivery systems (TDDS) are used as an alternative route to deliver drugs into the blood system for therapy.
33254969	1	74	theme	drug	116:119	arg1	systems					130:136	Transdermal drug delivery systems	104:136	Transdermal drug delivery systems (TDDS)	104:143	Transdermal drug delivery systems (TDDS) are used as an alternative route to deliver drugs into the blood system for therapy.
33254969	1	74	theme	drug	116:119	arg1	route					172:176	an alternative route	157:176	an alternative route to deliver drugs into the blood system for therapy	157:227	Transdermal drug delivery systems (TDDS) are used as an alternative route to deliver drugs into the blood system for therapy.
33254969	10	75	theme	efficient	1843:1851	arg1	features					1820:1827	the unique features	1809:1827	the unique features of PEAn	1809:1835	For the Dcf-loaded PEAn/dextran composite, the diffusion coefficient was enhanced by two orders of magnitude when the electric potential was applied, specifically illustrating the unique features of PEAn as an efficient drug encapsulation host without electric field, and as a drug release enhancer under electric field through the reduction reaction.
33254969	10	75	theme	efficient	1843:1851	arg1	host					1872:1875	an efficient drug encapsulation host	1840:1875	an efficient drug encapsulation host without electric field	1840:1898	For the Dcf-loaded PEAn/dextran composite, the diffusion coefficient was enhanced by two orders of magnitude when the electric potential was applied, specifically illustrating the unique features of PEAn as an efficient drug encapsulation host without electric field, and as a drug release enhancer under electric field through the reduction reaction.
33254969	10	76	theme	PEAn/dextran	1652:1663	arg1	composite					1665:1673	the Dcf-loaded PEAn/dextran composite	1637:1673	the Dcf-loaded PEAn/dextran composite	1637:1673	For the Dcf-loaded PEAn/dextran composite, the diffusion coefficient was enhanced by two orders of magnitude when the electric potential was applied, specifically illustrating the unique features of PEAn as an efficient drug encapsulation host without electric field, and as a drug release enhancer under electric field through the reduction reaction.
33254969	5	77	theme	7.4	777:779	arg1	pH					771:772	the pH	767:772	the pH of 7.4	767:779	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	5	78	theme	polymer	925:931	arg1	PEAn					933:936	the conductive polymer PEAn	910:936	the conductive polymer PEAn	910:936	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	3	79	theme	sodium	443:448	arg1	Dcf					456:458	Dcf	456:458	Dcf	456:458	The dextran hydrogels were prepared by the solution casting using trisodium trimetaphosphate (STMP) as the crosslinking agent, and diclofenac sodium salt (Dcf) as the anionic model drug.
33254969	3	79	theme	sodium	443:448	arg1	salt					450:453	diclofenac sodium salt	432:453	diclofenac sodium salt (Dcf)	432:459	The dextran hydrogels were prepared by the solution casting using trisodium trimetaphosphate (STMP) as the crosslinking agent, and diclofenac sodium salt (Dcf) as the anionic model drug.
33254969	10	80	theme	diffusion	1680:1688	arg1	coefficient					1690:1700	the diffusion coefficient	1676:1700	the diffusion coefficient	1676:1700	For the Dcf-loaded PEAn/dextran composite, the diffusion coefficient was enhanced by two orders of magnitude when the electric potential was applied, specifically illustrating the unique features of PEAn as an efficient drug encapsulation host without electric field, and as a drug release enhancer under electric field through the reduction reaction.
33254969	5	81	theme	electric	885:892	arg1	potentials					894:903	electric potentials	885:903	electric potentials	885:903	The in-vitro release of Dcf from the hydrogels was investigated using a modified Franz-Diffusion cell in a phosphate-buffered saline (PBS) solution at the pH of 7.4 and at 37 °C for a period of 24 h, under the effects of crosslinking ratios, dextran molecular weights, electric potentials, and the conductive polymer PEAn.
33254969	10	82	theme	encapsulation	1858:1870	arg1	features					1820:1827	the unique features	1809:1827	the unique features of PEAn	1809:1835	For the Dcf-loaded PEAn/dextran composite, the diffusion coefficient was enhanced by two orders of magnitude when the electric potential was applied, specifically illustrating the unique features of PEAn as an efficient drug encapsulation host without electric field, and as a drug release enhancer under electric field through the reduction reaction.
33254969	10	82	theme	encapsulation	1858:1870	arg1	host					1872:1875	an efficient drug encapsulation host	1840:1875	an efficient drug encapsulation host without electric field	1840:1898	For the Dcf-loaded PEAn/dextran composite, the diffusion coefficient was enhanced by two orders of magnitude when the electric potential was applied, specifically illustrating the unique features of PEAn as an efficient drug encapsulation host without electric field, and as a drug release enhancer under electric field through the reduction reaction.
33254969	8	83	theme	crosslinking	1352:1363	arg1	ratio					1365:1369	crosslinking ratio	1352:1369	crosslinking ratio	1352:1369	The Dcf diffusion coefficients from the dextran hydrogels without electrical potential increased with decreasing crosslinking ratio and molecular weight.
33254969	6	84	from	hydrogels	985:993	arg1	mechanism					951:959	The release mechanism	939:959	The release mechanism of Dcf from the dextran hydrogels and the composite without electrical potential	939:1040	The release mechanism of Dcf from the dextran hydrogels and the composite without electrical potential was the diffusion controlled mechanism or the Fickian diffusion.
33254969	6	84	from	hydrogels	985:993	arg1	mechanism					1071:1079	the diffusion controlled mechanism	1046:1079	the diffusion controlled mechanism	1046:1079	The release mechanism of Dcf from the dextran hydrogels and the composite without electrical potential was the diffusion controlled mechanism or the Fickian diffusion.
33254969	1	85	theme	blood	204:208	arg1	system					210:215	the blood system	200:215	the blood system for therapy	200:227	Transdermal drug delivery systems (TDDS) are used as an alternative route to deliver drugs into the blood system for therapy.
33254969	3	86	theme	model	476:480	arg1	drug					482:485	the anionic model drug	464:485	the anionic model drug	464:485	The dextran hydrogels were prepared by the solution casting using trisodium trimetaphosphate (STMP) as the crosslinking agent, and diclofenac sodium salt (Dcf) as the anionic model drug.
33254969	3	86	theme	model	476:480	arg1	trimetaphosphate					377:392	trisodium trimetaphosphate	367:392	trisodium trimetaphosphate (STMP)	367:399	The dextran hydrogels were prepared by the solution casting using trisodium trimetaphosphate (STMP) as the crosslinking agent, and diclofenac sodium salt (Dcf) as the anionic model drug.
34254604	4	0	theme	mechanical	666:675	arg1	properties					677:686	similar mechanical properties	658:686	similar mechanical properties (E∼ 60 kPa)	658:698	Hydrogel composites were shown to be easily injectable (F < 30 N), with fast hardening properties (<5 min), and similar mechanical properties (E∼ 60 kPa).
34254604	4	0	theme	mechanical	666:675	arg1	kPa					695:697	E∼ 60 kPa	689:697	E∼ 60 kPa	689:697	Hydrogel composites were shown to be easily injectable (F < 30 N), with fast hardening properties (<5 min), and similar mechanical properties (E∼ 60 kPa).
34254604	1	1	theme	phosphate	191:199	arg1	granules					207:214	Biphasic calcium phosphate (BCP) granules	174:214	Biphasic calcium phosphate (BCP) granules	174:214	Biphasic calcium phosphate (BCP) granules are osteoconductive biomaterials used in clinics to favor bone reconstruction.
34254604	1	1	theme	phosphate	191:199	arg1	biomaterials					236:247	osteoconductive biomaterials	220:247	osteoconductive biomaterials used in clinics to favor bone reconstruction	220:292	Biphasic calcium phosphate (BCP) granules are osteoconductive biomaterials used in clinics to favor bone reconstruction.
34254604	0	2	theme	improved	97:104	arg1	handling					106:113	improved handling	97:113	improved handling	97:113	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites with improved handling and biodegradability promote bone regeneration in rabbits.
34254604	4	3	theme	Hydrogel	546:553	arg1	composites					555:564	Hydrogel composites	546:564	Hydrogel composites	546:564	Hydrogel composites were shown to be easily injectable (F < 30 N), with fast hardening properties (<5 min), and similar mechanical properties (E∼ 60 kPa).
34254604	5	4	theme	Si-HPMC	854:860	arg1	gels					862:865	Si-HPMC gels	854:865	Si-HPMC gels	854:865	In vivo, both hydrogels were well tolerated by the host, but showed different biodegradability with Si-HA gels being partially degraded after 21d, while Si-HPMC gels remained stable.
34254604	9	5	theme	promising	1374:1382	arg1	composites					1359:1368	Si-HA/BCP composites	1349:1368	Si-HA/BCP composites	1349:1368	Our study demonstrates that the degradation rate is key to control bone regeneration and that Si-HA/BCP composites are promising biomaterials to regenerate bone defects.
34254604	9	5	theme	promising	1374:1382	arg1	biomaterials					1384:1395	promising biomaterials	1374:1395	promising biomaterials	1374:1395	Our study demonstrates that the degradation rate is key to control bone regeneration and that Si-HA/BCP composites are promising biomaterials to regenerate bone defects.
34254604	7	6	theme	degradation	1047:1057	arg1	lack					1039:1042	a lack	1037:1042	a lack of degradation	1037:1057	After 4 weeks, Si-HPMC/BCP led to poor bone healing due to a lack of degradation.
34254604	8	7	theme	granules	1236:1243	arg1	turnover					1245:1252	BCP granules turnover	1232:1252	BCP granules turnover	1232:1252	Conversely, Si-HA/BCP composites were fully degraded and beneficially influenced bone regeneration by increasing the space available for bone ingrowth, and by accelerating BCP granules turnover.
34254604	6	8	theme	rabbit	940:945	arg1	defects					947:953	critical size rabbit defects	926:953	critical size rabbit defects	926:953	Both composites were easily injected into critical size rabbit defects and remained cohesive.
34254604	0	9	theme	bone	144:147	arg1	regeneration					149:160	bone regeneration	144:160	bone regeneration	144:160	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites with improved handling and biodegradability promote bone regeneration in rabbits.
34254604	4	10	dep	injectable	590:599	arg1	F					602:602	F	602:602	F < 30 N	602:609	Hydrogel composites were shown to be easily injectable (F < 30 N), with fast hardening properties (<5 min), and similar mechanical properties (E∼ 60 kPa).
34254604	0	11	with	composites	81:90	arg1	biodegradability					119:134	biodegradability	119:134	biodegradability	119:134	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites with improved handling and biodegradability promote bone regeneration in rabbits.
34254604	0	11	with	composites	81:90	arg1	handling					106:113	improved handling	97:113	improved handling	97:113	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites with improved handling and biodegradability promote bone regeneration in rabbits.
34254604	3	12	theme	silanized	458:466	arg1	Si-HA					485:489	Si-HA	485:489	Si-HA	485:489	In this study, we incorporated BCP granules into in situ forming silanized hyaluronic acid (Si-HA) and hydroxypropylmethylcellulose (Si-HPMC) hydrogels.
34254604	3	12	theme	silanized	458:466	arg1	acid					479:482	silanized hyaluronic acid	458:482	silanized hyaluronic acid (Si-HA)	458:490	In this study, we incorporated BCP granules into in situ forming silanized hyaluronic acid (Si-HA) and hydroxypropylmethylcellulose (Si-HPMC) hydrogels.
34254604	2	13	theme	poor	300:303	arg1	cohesivity					305:314	poor cohesivity	300:314	poor cohesivity	300:314	Yet, poor cohesivity, injectability and mechanical properties restrain their use as bone fillers.
34254604	3	14	theme	hyaluronic	468:477	arg1	Si-HA					485:489	Si-HA	485:489	Si-HA	485:489	In this study, we incorporated BCP granules into in situ forming silanized hyaluronic acid (Si-HA) and hydroxypropylmethylcellulose (Si-HPMC) hydrogels.
34254604	3	14	theme	hyaluronic	468:477	arg1	acid					479:482	silanized hyaluronic acid	458:482	silanized hyaluronic acid (Si-HA)	458:490	In this study, we incorporated BCP granules into in situ forming silanized hyaluronic acid (Si-HA) and hydroxypropylmethylcellulose (Si-HPMC) hydrogels.
34254604	0	15	theme	silanized	11:19	arg1	composites					81:90	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites	0:90	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites with improved handling and biodegradability	0:134	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites with improved handling and biodegradability promote bone regeneration in rabbits.
34254604	9	16	theme	degradation	1287:1297	arg1	key					1307:1309	key	1307:1309	key	1307:1309	Our study demonstrates that the degradation rate is key to control bone regeneration and that Si-HA/BCP composites are promising biomaterials to regenerate bone defects.
34254604	9	16	theme	degradation	1287:1297	arg1	rate					1299:1302	the degradation rate	1283:1302	the degradation rate	1283:1302	Our study demonstrates that the degradation rate is key to control bone regeneration and that Si-HA/BCP composites are promising biomaterials to regenerate bone defects.
34254604	0	17	theme	Injectable	0:9	arg1	composites					81:90	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites	0:90	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites with improved handling and biodegradability	0:134	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites with improved handling and biodegradability promote bone regeneration in rabbits.
34254604	6	18	theme	size	935:938	arg1	defects					947:953	critical size rabbit defects	926:953	critical size rabbit defects	926:953	Both composites were easily injected into critical size rabbit defects and remained cohesive.
34254604	8	19	theme	bone	1197:1200	arg1	ingrowth					1202:1209	bone ingrowth	1197:1209	bone ingrowth	1197:1209	Conversely, Si-HA/BCP composites were fully degraded and beneficially influenced bone regeneration by increasing the space available for bone ingrowth, and by accelerating BCP granules turnover.
34254604	0	20	theme	acid	32:35	arg1	composites					81:90	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites	0:90	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites with improved handling and biodegradability	0:134	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites with improved handling and biodegradability promote bone regeneration in rabbits.
34254604	6	21	theme	critical	926:933	arg1	defects					947:953	critical size rabbit defects	926:953	critical size rabbit defects	926:953	Both composites were easily injected into critical size rabbit defects and remained cohesive.
34254604	7	22	theme	bone	1017:1020	arg1	healing					1022:1028	poor bone healing	1012:1028	poor bone healing	1012:1028	After 4 weeks, Si-HPMC/BCP led to poor bone healing due to a lack of degradation.
34254604	2	23	theme	mechanical	335:344	arg1	properties					346:355	mechanical properties	335:355	mechanical properties	335:355	Yet, poor cohesivity, injectability and mechanical properties restrain their use as bone fillers.
34254604	1	24	theme	osteoconductive	220:234	arg1	granules					207:214	Biphasic calcium phosphate (BCP) granules	174:214	Biphasic calcium phosphate (BCP) granules	174:214	Biphasic calcium phosphate (BCP) granules are osteoconductive biomaterials used in clinics to favor bone reconstruction.
34254604	1	24	theme	osteoconductive	220:234	arg1	biomaterials					236:247	osteoconductive biomaterials	220:247	osteoconductive biomaterials used in clinics to favor bone reconstruction	220:292	Biphasic calcium phosphate (BCP) granules are osteoconductive biomaterials used in clinics to favor bone reconstruction.
34254604	0	25	theme	hyaluronic	21:30	arg1	composites					81:90	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites	0:90	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites with improved handling and biodegradability	0:134	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites with improved handling and biodegradability promote bone regeneration in rabbits.
34254604	4	26	theme	hardening	623:631	arg1	properties					633:642	fast hardening properties	618:642	fast hardening properties (<5 min)	618:651	Hydrogel composites were shown to be easily injectable (F < 30 N), with fast hardening properties (<5 min), and similar mechanical properties (E∼ 60 kPa).
34254604	4	26	theme	hardening	623:631	arg1	min					648:650	<5 min	645:650	<5 min	645:650	Hydrogel composites were shown to be easily injectable (F < 30 N), with fast hardening properties (<5 min), and similar mechanical properties (E∼ 60 kPa).
34254604	0	27	theme	calcium	55:61	arg1	composites					81:90	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites	0:90	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites with improved handling and biodegradability	0:134	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites with improved handling and biodegradability promote bone regeneration in rabbits.
34254604	4	28	theme	E∼	689:690	arg1	properties					677:686	similar mechanical properties	658:686	similar mechanical properties (E∼ 60 kPa)	658:698	Hydrogel composites were shown to be easily injectable (F < 30 N), with fast hardening properties (<5 min), and similar mechanical properties (E∼ 60 kPa).
34254604	4	28	theme	E∼	689:690	arg1	kPa					695:697	E∼ 60 kPa	689:697	E∼ 60 kPa	689:697	Hydrogel composites were shown to be easily injectable (F < 30 N), with fast hardening properties (<5 min), and similar mechanical properties (E∼ 60 kPa).
34254604	8	29	theme	BCP	1232:1234	arg1	granules					1236:1243	BCP granules	1232:1243	BCP granules turnover	1232:1252	Conversely, Si-HA/BCP composites were fully degraded and beneficially influenced bone regeneration by increasing the space available for bone ingrowth, and by accelerating BCP granules turnover.
34254604	5	30	theme	Si-HA	801:805	arg1	gels					807:810	Si-HA gels	801:810	Si-HA gels	801:810	In vivo, both hydrogels were well tolerated by the host, but showed different biodegradability with Si-HA gels being partially degraded after 21d, while Si-HPMC gels remained stable.
34254604	0	31	theme	hydrogel/biphasic	37:53	arg1	composites					81:90	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites	0:90	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites with improved handling and biodegradability	0:134	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites with improved handling and biodegradability promote bone regeneration in rabbits.
34254604	7	32	theme	poor	1012:1015	arg1	healing					1022:1028	poor bone healing	1012:1028	poor bone healing	1012:1028	After 4 weeks, Si-HPMC/BCP led to poor bone healing due to a lack of degradation.
34254604	9	33	theme	Si-HA/BCP	1349:1357	arg1	composites					1359:1368	Si-HA/BCP composites	1349:1368	Si-HA/BCP composites	1349:1368	Our study demonstrates that the degradation rate is key to control bone regeneration and that Si-HA/BCP composites are promising biomaterials to regenerate bone defects.
34254604	9	33	theme	Si-HA/BCP	1349:1357	arg1	biomaterials					1384:1395	promising biomaterials	1374:1395	promising biomaterials	1374:1395	Our study demonstrates that the degradation rate is key to control bone regeneration and that Si-HA/BCP composites are promising biomaterials to regenerate bone defects.
34254604	8	34	theme	bone	1141:1144	arg1	regeneration					1146:1157	bone regeneration	1141:1157	bone regeneration	1141:1157	Conversely, Si-HA/BCP composites were fully degraded and beneficially influenced bone regeneration by increasing the space available for bone ingrowth, and by accelerating BCP granules turnover.
34254604	0	35	theme	granule	73:79	arg1	composites					81:90	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites	0:90	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites with improved handling and biodegradability	0:134	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites with improved handling and biodegradability promote bone regeneration in rabbits.
34254604	4	36	dep	F	602:602	arg1	N					609:609	< 30 N	604:609	F < 30 N	602:609	Hydrogel composites were shown to be easily injectable (F < 30 N), with fast hardening properties (<5 min), and similar mechanical properties (E∼ 60 kPa).
34254604	2	37	theme	bone	379:382	arg1	fillers					384:390	bone fillers	379:390	bone fillers	379:390	Yet, poor cohesivity, injectability and mechanical properties restrain their use as bone fillers.
34254604	0	38	theme	phosphate	63:71	arg1	composites					81:90	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites	0:90	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites with improved handling and biodegradability	0:134	Injectable silanized hyaluronic acid hydrogel/biphasic calcium phosphate granule composites with improved handling and biodegradability promote bone regeneration in rabbits.
34254604	9	39	theme	bone	1322:1325	arg1	regeneration					1327:1338	bone regeneration	1322:1338	bone regeneration	1322:1338	Our study demonstrates that the degradation rate is key to control bone regeneration and that Si-HA/BCP composites are promising biomaterials to regenerate bone defects.
34254604	3	40	theme	Si-HPMC	526:532	arg1	hydrogels					535:543	hydroxypropylmethylcellulose (Si-HPMC) hydrogels	496:543	hydroxypropylmethylcellulose (Si-HPMC) hydrogels	496:543	In this study, we incorporated BCP granules into in situ forming silanized hyaluronic acid (Si-HA) and hydroxypropylmethylcellulose (Si-HPMC) hydrogels.
34254604	4	41	theme	similar	658:664	arg1	properties					677:686	similar mechanical properties	658:686	similar mechanical properties (E∼ 60 kPa)	658:698	Hydrogel composites were shown to be easily injectable (F < 30 N), with fast hardening properties (<5 min), and similar mechanical properties (E∼ 60 kPa).
34254604	4	41	theme	similar	658:664	arg1	kPa					695:697	E∼ 60 kPa	689:697	E∼ 60 kPa	689:697	Hydrogel composites were shown to be easily injectable (F < 30 N), with fast hardening properties (<5 min), and similar mechanical properties (E∼ 60 kPa).
34254604	8	42	dep	influenced	1130:1139	arg1	accelerating					1219:1230	accelerating	1219:1230	accelerating BCP granules turnover	1219:1252	Conversely, Si-HA/BCP composites were fully degraded and beneficially influenced bone regeneration by increasing the space available for bone ingrowth, and by accelerating BCP granules turnover.
34254604	8	42	dep	influenced	1130:1139	arg1	increasing					1162:1171	increasing	1162:1171	increasing the space available for bone ingrowth	1162:1209	Conversely, Si-HA/BCP composites were fully degraded and beneficially influenced bone regeneration by increasing the space available for bone ingrowth, and by accelerating BCP granules turnover.
34254604	3	43	theme	hydroxypropylmethylcellulose	496:523	arg1	hydrogels					535:543	hydroxypropylmethylcellulose (Si-HPMC) hydrogels	496:543	hydroxypropylmethylcellulose (Si-HPMC) hydrogels	496:543	In this study, we incorporated BCP granules into in situ forming silanized hyaluronic acid (Si-HA) and hydroxypropylmethylcellulose (Si-HPMC) hydrogels.
34254604	8	44	theme	available	1183:1191	arg1	space					1177:1181	the space	1173:1181	the space available for bone ingrowth	1173:1209	Conversely, Si-HA/BCP composites were fully degraded and beneficially influenced bone regeneration by increasing the space available for bone ingrowth, and by accelerating BCP granules turnover.
34254604	8	45	theme	Si-HA/BCP	1072:1080	arg1	composites					1082:1091	Si-HA/BCP composites	1072:1091	Si-HA/BCP composites	1072:1091	Conversely, Si-HA/BCP composites were fully degraded and beneficially influenced bone regeneration by increasing the space available for bone ingrowth, and by accelerating BCP granules turnover.
34254604	1	46	theme	Biphasic	174:181	arg1	BCP					202:204	BCP	202:204	BCP	202:204	Biphasic calcium phosphate (BCP) granules are osteoconductive biomaterials used in clinics to favor bone reconstruction.
34254604	1	46	theme	Biphasic	174:181	arg1	phosphate					191:199	Biphasic calcium phosphate	174:199	Biphasic calcium phosphate (BCP) granules	174:214	Biphasic calcium phosphate (BCP) granules are osteoconductive biomaterials used in clinics to favor bone reconstruction.
34254604	9	47	theme	bone	1411:1414	arg1	defects					1416:1422	bone defects	1411:1422	bone defects	1411:1422	Our study demonstrates that the degradation rate is key to control bone regeneration and that Si-HA/BCP composites are promising biomaterials to regenerate bone defects.
34254604	4	48	theme	fast	618:621	arg1	properties					633:642	fast hardening properties	618:642	fast hardening properties (<5 min)	618:651	Hydrogel composites were shown to be easily injectable (F < 30 N), with fast hardening properties (<5 min), and similar mechanical properties (E∼ 60 kPa).
34254604	4	48	theme	fast	618:621	arg1	min					648:650	<5 min	645:650	<5 min	645:650	Hydrogel composites were shown to be easily injectable (F < 30 N), with fast hardening properties (<5 min), and similar mechanical properties (E∼ 60 kPa).
34254604	3	49	theme	BCP	424:426	arg1	granules					428:435	BCP granules	424:435	BCP granules	424:435	In this study, we incorporated BCP granules into in situ forming silanized hyaluronic acid (Si-HA) and hydroxypropylmethylcellulose (Si-HPMC) hydrogels.
34254604	5	50	theme	different	769:777	arg1	biodegradability					779:794	different biodegradability	769:794	different biodegradability	769:794	In vivo, both hydrogels were well tolerated by the host, but showed different biodegradability with Si-HA gels being partially degraded after 21d, while Si-HPMC gels remained stable.
34254604	1	51	theme	calcium	183:189	arg1	BCP					202:204	BCP	202:204	BCP	202:204	Biphasic calcium phosphate (BCP) granules are osteoconductive biomaterials used in clinics to favor bone reconstruction.
34254604	1	51	theme	calcium	183:189	arg1	phosphate					191:199	Biphasic calcium phosphate	174:199	Biphasic calcium phosphate (BCP) granules	174:214	Biphasic calcium phosphate (BCP) granules are osteoconductive biomaterials used in clinics to favor bone reconstruction.
34254604	1	52	theme	bone	274:277	arg1	reconstruction					279:292	bone reconstruction	274:292	bone reconstruction	274:292	Biphasic calcium phosphate (BCP) granules are osteoconductive biomaterials used in clinics to favor bone reconstruction.
34515908	8	0	theme	heparin	1209:1215	arg1	groups					1217:1222	heparin groups	1209:1222	heparin groups	1209:1222	In addition, compared to heparin groups, the non-desulfated gs-HP showed no significantly different MB-SO3- ratio with heparin.
34515908	7	1	theme	sulfation	1010:1018	arg1	position					1020:1027	a distinct sulfation position	999:1027	a distinct sulfation position	999:1027	Although in a distinct sulfation position, the MB-SO3- ratio of 6-OdeS and N-deS-Acetyl was almost equal, which agreed with the comparable total desulfation degree between 6-OdeS and N-deS-Acetyl.
34515908	7	2	theme	comparable	1115:1124	arg1	degree					1144:1149	the comparable total desulfation degree	1111:1149	the comparable total desulfation degree between 6-OdeS and N-deS-Acetyl	1111:1181	Although in a distinct sulfation position, the MB-SO3- ratio of 6-OdeS and N-deS-Acetyl was almost equal, which agreed with the comparable total desulfation degree between 6-OdeS and N-deS-Acetyl.
34515908	0	3	theme	sulfate	64:70	arg1	level					72:76	its sulfate level	60:76	its sulfate level rather than its sulfation location or basic saccharide structure	60:141	Binding ability of methylene blue with heparin dependent on its sulfate level rather than its sulfation location or basic saccharide structure.
34515908	5	4	from	6-O	716:718	arg1	heparin					746:752	N-acetylated heparin	733:752	N-acetylated heparin (N-deS-Acetyl)	733:767	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	5	4	from	6-O	716:718	arg1	N-deS-Acetyl					755:766	N-deS-Acetyl	755:766	N-deS-Acetyl	755:766	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	5	4	from	6-O	716:718	arg1	derivatives					680:690	the two selectively desulfated heparin derivatives	641:690	the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl)	641:767	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	5	4	from	6-O	716:718	arg1	elimination					701:711	sulfate elimination	693:711	sulfate elimination at 6-O (6-OdeS)	693:727	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	1	5	theme	blue	154:157	arg1	Methylene					144:152	Methylene blue	144:157	Methylene blue (MB)	144:162	Methylene blue (MB) is one of the most common cationic dyes to detect heparin.
34515908	1	5	theme	blue	154:157	arg1	MB					160:161	MB	160:161	MB	160:161	Methylene blue (MB) is one of the most common cationic dyes to detect heparin.
34515908	9	6	theme	glycan	1387:1392	arg1	composition					1394:1404	glycan composition	1387:1404	glycan composition	1387:1404	The above results demonstrated that compared with the sulfate location and glycan composition of heparin, the content of sulfate was the most essential factor for the MB binding.
34515908	9	7	theme	sulfate	1433:1439	arg1	factor					1464:1469	the most essential factor	1445:1469	the most essential factor for the MB binding	1445:1488	The above results demonstrated that compared with the sulfate location and glycan composition of heparin, the content of sulfate was the most essential factor for the MB binding.
34515908	9	7	theme	sulfate	1433:1439	arg1	content					1422:1428	the content	1418:1428	the content of sulfate	1418:1439	The above results demonstrated that compared with the sulfate location and glycan composition of heparin, the content of sulfate was the most essential factor for the MB binding.
34515908	9	8	theme	above	1316:1320	arg1	results					1322:1328	The above results	1312:1328	The above results	1312:1328	The above results demonstrated that compared with the sulfate location and glycan composition of heparin, the content of sulfate was the most essential factor for the MB binding.
34515908	5	9	theme	desulfated	661:670	arg1	heparin					672:678	selectively desulfated heparin	649:678	the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl)	641:767	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	7	10	theme	distinct	1001:1008	arg1	position					1020:1027	a distinct sulfation position	999:1027	a distinct sulfation position	999:1027	Although in a distinct sulfation position, the MB-SO3- ratio of 6-OdeS and N-deS-Acetyl was almost equal, which agreed with the comparable total desulfation degree between 6-OdeS and N-deS-Acetyl.
34515908	7	11	theme	total	1126:1130	arg1	degree					1144:1149	the comparable total desulfation degree	1111:1149	the comparable total desulfation degree between 6-OdeS and N-deS-Acetyl	1111:1181	Although in a distinct sulfation position, the MB-SO3- ratio of 6-OdeS and N-deS-Acetyl was almost equal, which agreed with the comparable total desulfation degree between 6-OdeS and N-deS-Acetyl.
34515908	6	12	theme	derivative	891:900	arg1	2-OdeS					903:908	2-O heparin derivative (2-OdeS)	879:909	2-O heparin derivative (2-OdeS)	879:909	For the not fully diminished sulfate at 2-O heparin derivative (2-OdeS), the MB-SO3- ratio of 2-OdeS was between 6-OdeS, N-deS-Acetlyl and heparin.
34515908	0	13	theme	sulfation	94:102	arg1	location					104:111	its sulfation location	90:111	its sulfation location	90:111	Binding ability of methylene blue with heparin dependent on its sulfate level rather than its sulfation location or basic saccharide structure.
34515908	2	14	with	MB	335:336	arg1	heparin					366:372	selectively desulfated heparin	343:372	selectively desulfated heparin	343:372	As the sulfate residue presented in heparin was the main contributor to bind with MB, the UV performance of the MB with selectively desulfated heparin derivatives was investigated.
34515908	9	15	theme	sulfate	1366:1372	arg1	location					1374:1381	sulfate location	1366:1381	sulfate location	1366:1381	The above results demonstrated that compared with the sulfate location and glycan composition of heparin, the content of sulfate was the most essential factor for the MB binding.
34515908	2	16	theme	sulfate	230:236	arg1	residue					238:244	the sulfate residue	226:244	the sulfate residue presented in heparin	226:265	As the sulfate residue presented in heparin was the main contributor to bind with MB, the UV performance of the MB with selectively desulfated heparin derivatives was investigated.
34515908	2	16	theme	sulfate	230:236	arg1	contributor					280:290	the main contributor	271:290	the main contributor to bind with MB	271:306	As the sulfate residue presented in heparin was the main contributor to bind with MB, the UV performance of the MB with selectively desulfated heparin derivatives was investigated.
34515908	5	17	theme	MB	774:775	arg1	higher					814:819	higher	814:819	higher	814:819	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	5	17	theme	MB	774:775	arg1	ratios					788:793	the MB to sulfate ratios	770:793	the MB to sulfate ratios	770:793	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	5	18	theme	N-acetylated	733:744	arg1	N-deS-Acetyl					755:766	N-deS-Acetyl	755:766	N-deS-Acetyl	755:766	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	5	18	theme	N-acetylated	733:744	arg1	derivatives					680:690	the two selectively desulfated heparin derivatives	641:690	the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl)	641:767	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	5	18	theme	N-acetylated	733:744	arg1	heparin					746:752	N-acetylated heparin	733:752	N-acetylated heparin (N-deS-Acetyl)	733:767	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	5	19	theme	heparin	672:678	arg1	elimination					701:711	sulfate elimination	693:711	sulfate elimination at 6-O (6-OdeS)	693:727	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	5	19	theme	heparin	672:678	arg1	derivatives					680:690	the two selectively desulfated heparin derivatives	641:690	the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl)	641:767	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	5	19	theme	heparin	672:678	arg1	heparin					746:752	N-acetylated heparin	733:752	N-acetylated heparin (N-deS-Acetyl)	733:767	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	9	20	theme	essential	1454:1462	arg1	factor					1464:1469	the most essential factor	1445:1469	the most essential factor for the MB binding	1445:1488	The above results demonstrated that compared with the sulfate location and glycan composition of heparin, the content of sulfate was the most essential factor for the MB binding.
34515908	9	20	theme	essential	1454:1462	arg1	content					1422:1428	the content	1418:1428	the content of sulfate	1418:1439	The above results demonstrated that compared with the sulfate location and glycan composition of heparin, the content of sulfate was the most essential factor for the MB binding.
34515908	5	21	theme	sulfate	780:786	arg1	higher					814:819	higher	814:819	higher	814:819	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	5	21	theme	sulfate	780:786	arg1	ratios					788:793	the MB to sulfate ratios	770:793	the MB to sulfate ratios	770:793	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	0	22	theme	Binding	0:6	arg1	ability					8:14	Binding ability	0:14	Binding ability of methylene blue with heparin dependent on its sulfate level rather than its sulfation location or basic saccharide structure.	0:142	Binding ability of methylene blue with heparin dependent on its sulfate level rather than its sulfation location or basic saccharide structure.
34515908	6	23	theme	heparin	883:889	arg1	2-OdeS					903:908	2-O heparin derivative (2-OdeS)	879:909	2-O heparin derivative (2-OdeS)	879:909	For the not fully diminished sulfate at 2-O heparin derivative (2-OdeS), the MB-SO3- ratio of 2-OdeS was between 6-OdeS, N-deS-Acetlyl and heparin.
34515908	6	24	from	2-OdeS	903:908	arg1	sulfate					868:874	the not fully diminished sulfate	843:874	the not fully diminished sulfate at 2-O heparin derivative (2-OdeS)	843:909	For the not fully diminished sulfate at 2-O heparin derivative (2-OdeS), the MB-SO3- ratio of 2-OdeS was between 6-OdeS, N-deS-Acetlyl and heparin.
34515908	2	25	theme	UV	313:314	arg1	performance					316:326	the UV performance	309:326	the UV performance of the MB with selectively desulfated heparin derivatives	309:384	As the sulfate residue presented in heparin was the main contributor to bind with MB, the UV performance of the MB with selectively desulfated heparin derivatives was investigated.
34515908	0	26	theme	methylene	19:27	arg1	ability					8:14	Binding ability	0:14	Binding ability of methylene blue with heparin dependent on its sulfate level rather than its sulfation location or basic saccharide structure.	0:142	Binding ability of methylene blue with heparin dependent on its sulfate level rather than its sulfation location or basic saccharide structure.
34515908	6	27	theme	2-O	879:881	arg1	2-OdeS					903:908	2-O heparin derivative (2-OdeS)	879:909	2-O heparin derivative (2-OdeS)	879:909	For the not fully diminished sulfate at 2-O heparin derivative (2-OdeS), the MB-SO3- ratio of 2-OdeS was between 6-OdeS, N-deS-Acetlyl and heparin.
34515908	3	28	from	residue	434:440	arg1	analogues					463:471	different heparin analogues	445:471	different heparin analogues	445:471	It was found that the sulfate residue in different heparin analogues did not show the equal ability to attract MB binding.
34515908	3	29	theme	equal	490:494	arg1	ability					496:502	the equal ability	486:502	the equal ability to attract MB binding	486:524	It was found that the sulfate residue in different heparin analogues did not show the equal ability to attract MB binding.
34515908	9	30	theme	MB	1479:1480	arg1	binding					1482:1488	the MB binding	1475:1488	the MB binding	1475:1488	The above results demonstrated that compared with the sulfate location and glycan composition of heparin, the content of sulfate was the most essential factor for the MB binding.
34515908	2	31	theme	desulfated	355:364	arg1	heparin					366:372	selectively desulfated heparin	343:372	selectively desulfated heparin	343:372	As the sulfate residue presented in heparin was the main contributor to bind with MB, the UV performance of the MB with selectively desulfated heparin derivatives was investigated.
34515908	7	32	theme	6-OdeS	1051:1056	arg1	equal					1086:1090	equal	1086:1090	equal	1086:1090	Although in a distinct sulfation position, the MB-SO3- ratio of 6-OdeS and N-deS-Acetyl was almost equal, which agreed with the comparable total desulfation degree between 6-OdeS and N-deS-Acetyl.
34515908	7	32	theme	6-OdeS	1051:1056	arg1	ratio					1042:1046	the MB-SO3- ratio	1030:1046	the MB-SO3- ratio of 6-OdeS and N-deS-Acetyl	1030:1073	Although in a distinct sulfation position, the MB-SO3- ratio of 6-OdeS and N-deS-Acetyl was almost equal, which agreed with the comparable total desulfation degree between 6-OdeS and N-deS-Acetyl.
34515908	8	33	theme	MB-SO3-	1284:1290	arg1	ratio					1292:1296	no significantly different MB-SO3- ratio	1257:1296	no significantly different MB-SO3- ratio	1257:1296	In addition, compared to heparin groups, the non-desulfated gs-HP showed no significantly different MB-SO3- ratio with heparin.
34515908	3	34	theme	different	445:453	arg1	analogues					463:471	different heparin analogues	445:471	different heparin analogues	445:471	It was found that the sulfate residue in different heparin analogues did not show the equal ability to attract MB binding.
34515908	7	35	theme	desulfation	1132:1142	arg1	degree					1144:1149	the comparable total desulfation degree	1111:1149	the comparable total desulfation degree between 6-OdeS and N-deS-Acetyl	1111:1181	Although in a distinct sulfation position, the MB-SO3- ratio of 6-OdeS and N-deS-Acetyl was almost equal, which agreed with the comparable total desulfation degree between 6-OdeS and N-deS-Acetyl.
34515908	7	36	theme	MB-SO3-	1034:1040	arg1	equal					1086:1090	equal	1086:1090	equal	1086:1090	Although in a distinct sulfation position, the MB-SO3- ratio of 6-OdeS and N-deS-Acetyl was almost equal, which agreed with the comparable total desulfation degree between 6-OdeS and N-deS-Acetyl.
34515908	7	36	theme	MB-SO3-	1034:1040	arg1	ratio					1042:1046	the MB-SO3- ratio	1030:1046	the MB-SO3- ratio of 6-OdeS and N-deS-Acetyl	1030:1073	Although in a distinct sulfation position, the MB-SO3- ratio of 6-OdeS and N-deS-Acetyl was almost equal, which agreed with the comparable total desulfation degree between 6-OdeS and N-deS-Acetyl.
34515908	0	37	theme	saccharide	122:131	arg1	structure					133:141	basic saccharide structure	116:141	basic saccharide structure	116:141	Binding ability of methylene blue with heparin dependent on its sulfate level rather than its sulfation location or basic saccharide structure.
34515908	7	38	theme	N-deS-Acetyl	1062:1073	arg1	equal					1086:1090	equal	1086:1090	equal	1086:1090	Although in a distinct sulfation position, the MB-SO3- ratio of 6-OdeS and N-deS-Acetyl was almost equal, which agreed with the comparable total desulfation degree between 6-OdeS and N-deS-Acetyl.
34515908	7	38	theme	N-deS-Acetyl	1062:1073	arg1	ratio					1042:1046	the MB-SO3- ratio	1030:1046	the MB-SO3- ratio of 6-OdeS and N-deS-Acetyl	1030:1073	Although in a distinct sulfation position, the MB-SO3- ratio of 6-OdeS and N-deS-Acetyl was almost equal, which agreed with the comparable total desulfation degree between 6-OdeS and N-deS-Acetyl.
34515908	4	39	dep	heparin	574:580	arg1	the					570:572	the	570:572	the	570:572	The stoichiometry of sulfate with MB among the heparin and derivatives was verified as a non-constant number.
34515908	6	40	theme	2-OdeS	933:938	arg1	ratio					924:928	the MB-SO3- ratio	912:928	the MB-SO3- ratio of 2-OdeS	912:938	For the not fully diminished sulfate at 2-O heparin derivative (2-OdeS), the MB-SO3- ratio of 2-OdeS was between 6-OdeS, N-deS-Acetlyl and heparin.
34515908	0	41	theme	blue	29:32	arg1	methylene					19:27	methylene blue	19:32	methylene blue	19:32	Binding ability of methylene blue with heparin dependent on its sulfate level rather than its sulfation location or basic saccharide structure.
34515908	6	42	theme	diminished	857:866	arg1	sulfate					868:874	the not fully diminished sulfate	843:874	the not fully diminished sulfate at 2-O heparin derivative (2-OdeS)	843:909	For the not fully diminished sulfate at 2-O heparin derivative (2-OdeS), the MB-SO3- ratio of 2-OdeS was between 6-OdeS, N-deS-Acetlyl and heparin.
34515908	0	43	theme	basic	116:120	arg1	structure					133:141	basic saccharide structure	116:141	basic saccharide structure	116:141	Binding ability of methylene blue with heparin dependent on its sulfate level rather than its sulfation location or basic saccharide structure.
34515908	7	44	from	equal	1086:1090	arg1	position					1020:1027	a distinct sulfation position	999:1027	a distinct sulfation position	999:1027	Although in a distinct sulfation position, the MB-SO3- ratio of 6-OdeS and N-deS-Acetyl was almost equal, which agreed with the comparable total desulfation degree between 6-OdeS and N-deS-Acetyl.
34515908	2	45	theme	derivatives	374:384	arg1	performance					316:326	the UV performance	309:326	the UV performance of the MB with selectively desulfated heparin derivatives	309:384	As the sulfate residue presented in heparin was the main contributor to bind with MB, the UV performance of the MB with selectively desulfated heparin derivatives was investigated.
34515908	2	46	theme	MB	335:336	arg1	derivatives					374:384	the MB with selectively desulfated heparin derivatives	331:384	the MB with selectively desulfated heparin derivatives	331:384	As the sulfate residue presented in heparin was the main contributor to bind with MB, the UV performance of the MB with selectively desulfated heparin derivatives was investigated.
34515908	0	47	theme	dependent	47:55	arg1	heparin					39:45	heparin	39:45	heparin dependent on its sulfate level rather than its sulfation location or basic saccharide structure	39:141	Binding ability of methylene blue with heparin dependent on its sulfate level rather than its sulfation location or basic saccharide structure.
34515908	6	48	dep	fully	851:855	arg1	not					847:849	not	847:849	not	847:849	For the not fully diminished sulfate at 2-O heparin derivative (2-OdeS), the MB-SO3- ratio of 2-OdeS was between 6-OdeS, N-deS-Acetlyl and heparin.
34515908	3	49	theme	MB	515:516	arg1	binding					518:524	MB binding	515:524	MB binding	515:524	It was found that the sulfate residue in different heparin analogues did not show the equal ability to attract MB binding.
34515908	7	50	from	position	1020:1027	arg1	equal					1086:1090	equal	1086:1090	equal	1086:1090	Although in a distinct sulfation position, the MB-SO3- ratio of 6-OdeS and N-deS-Acetyl was almost equal, which agreed with the comparable total desulfation degree between 6-OdeS and N-deS-Acetyl.
34515908	7	50	from	position	1020:1027	arg1	ratio					1042:1046	the MB-SO3- ratio	1030:1046	the MB-SO3- ratio of 6-OdeS and N-deS-Acetyl	1030:1073	Although in a distinct sulfation position, the MB-SO3- ratio of 6-OdeS and N-deS-Acetyl was almost equal, which agreed with the comparable total desulfation degree between 6-OdeS and N-deS-Acetyl.
34515908	3	51	theme	sulfate	426:432	arg1	residue					434:440	the sulfate residue	422:440	the sulfate residue in different heparin analogues	422:471	It was found that the sulfate residue in different heparin analogues did not show the equal ability to attract MB binding.
34515908	9	52	theme	heparin	1409:1415	arg1	location					1374:1381	sulfate location	1366:1381	sulfate location	1366:1381	The above results demonstrated that compared with the sulfate location and glycan composition of heparin, the content of sulfate was the most essential factor for the MB binding.
34515908	9	52	theme	heparin	1409:1415	arg1	composition					1394:1404	glycan composition	1387:1404	glycan composition	1387:1404	The above results demonstrated that compared with the sulfate location and glycan composition of heparin, the content of sulfate was the most essential factor for the MB binding.
34515908	5	53	dep	derivatives	680:690	arg1	elimination					701:711	sulfate elimination	693:711	sulfate elimination at 6-O (6-OdeS)	693:727	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	5	53	dep	derivatives	680:690	arg1	N-deS-Acetyl					755:766	N-deS-Acetyl	755:766	N-deS-Acetyl	755:766	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	5	53	dep	derivatives	680:690	arg1	derivatives					680:690	the two selectively desulfated heparin derivatives	641:690	the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl)	641:767	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	5	53	dep	derivatives	680:690	arg1	heparin					746:752	N-acetylated heparin	733:752	N-acetylated heparin (N-deS-Acetyl)	733:767	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	4	54	with	stoichiometry	531:543	arg1	MB					561:562	MB	561:562	MB among the heparin and derivatives	561:596	The stoichiometry of sulfate with MB among the heparin and derivatives was verified as a non-constant number.
34515908	0	55	with	ability	8:14	arg1	heparin					39:45	heparin	39:45	heparin dependent on its sulfate level rather than its sulfation location or basic saccharide structure	39:141	Binding ability of methylene blue with heparin dependent on its sulfate level rather than its sulfation location or basic saccharide structure.
34515908	6	56	theme	MB-SO3-	916:922	arg1	ratio					924:928	the MB-SO3- ratio	912:928	the MB-SO3- ratio of 2-OdeS	912:938	For the not fully diminished sulfate at 2-O heparin derivative (2-OdeS), the MB-SO3- ratio of 2-OdeS was between 6-OdeS, N-deS-Acetlyl and heparin.
34515908	9	57	dep	location	1374:1381	arg1	the					1362:1364	the	1362:1364	the	1362:1364	The above results demonstrated that compared with the sulfate location and glycan composition of heparin, the content of sulfate was the most essential factor for the MB binding.
34515908	5	58	dep	ratios	788:793	arg1	to					777:778	to	777:778	to	777:778	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	4	59	theme	non-constant	616:627	arg1	number					629:634	a non-constant number	614:634	a non-constant number	614:634	The stoichiometry of sulfate with MB among the heparin and derivatives was verified as a non-constant number.
34515908	4	59	theme	non-constant	616:627	arg1	stoichiometry					531:543	The stoichiometry	527:543	The stoichiometry of sulfate with MB among the heparin and derivatives	527:596	The stoichiometry of sulfate with MB among the heparin and derivatives was verified as a non-constant number.
34515908	1	60	theme	common	183:188	arg1	dyes					199:202	the most common cationic dyes	174:202	the most common cationic dyes	174:202	Methylene blue (MB) is one of the most common cationic dyes to detect heparin.
34515908	8	61	theme	non-desulfated	1229:1242	arg1	gs-HP					1244:1248	the non-desulfated gs-HP	1225:1248	the non-desulfated gs-HP	1225:1248	In addition, compared to heparin groups, the non-desulfated gs-HP showed no significantly different MB-SO3- ratio with heparin.
34515908	2	62	theme	main	275:278	arg1	residue					238:244	the sulfate residue	226:244	the sulfate residue presented in heparin	226:265	As the sulfate residue presented in heparin was the main contributor to bind with MB, the UV performance of the MB with selectively desulfated heparin derivatives was investigated.
34515908	2	62	theme	main	275:278	arg1	contributor					280:290	the main contributor	271:290	the main contributor to bind with MB	271:306	As the sulfate residue presented in heparin was the main contributor to bind with MB, the UV performance of the MB with selectively desulfated heparin derivatives was investigated.
34515908	1	63	theme	cationic	190:197	arg1	dyes					199:202	the most common cationic dyes	174:202	the most common cationic dyes	174:202	Methylene blue (MB) is one of the most common cationic dyes to detect heparin.
34515908	8	64	theme	different	1274:1282	arg1	ratio					1292:1296	no significantly different MB-SO3- ratio	1257:1296	no significantly different MB-SO3- ratio	1257:1296	In addition, compared to heparin groups, the non-desulfated gs-HP showed no significantly different MB-SO3- ratio with heparin.
34515908	3	65	theme	heparin	455:461	arg1	analogues					463:471	different heparin analogues	445:471	different heparin analogues	445:471	It was found that the sulfate residue in different heparin analogues did not show the equal ability to attract MB binding.
34515908	1	66	theme	dyes	199:202	arg1	dyes					199:202	the most common cationic dyes	174:202	the most common cationic dyes	174:202	Methylene blue (MB) is one of the most common cationic dyes to detect heparin.
34515908	1	66	theme	dyes	199:202	arg1	one					167:169	one	167:169	one	167:169	Methylene blue (MB) is one of the most common cationic dyes to detect heparin.
34515908	5	67	theme	sulfate	693:699	arg1	derivatives					680:690	the two selectively desulfated heparin derivatives	641:690	the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl)	641:767	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	5	67	theme	sulfate	693:699	arg1	elimination					701:711	sulfate elimination	693:711	sulfate elimination at 6-O (6-OdeS)	693:727	For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acetyl), the MB to sulfate ratios were significantly higher than for heparin.
34515908	4	68	theme	sulfate	548:554	arg1	number					629:634	a non-constant number	614:634	a non-constant number	614:634	The stoichiometry of sulfate with MB among the heparin and derivatives was verified as a non-constant number.
34515908	4	68	theme	sulfate	548:554	arg1	stoichiometry					531:543	The stoichiometry	527:543	The stoichiometry of sulfate with MB among the heparin and derivatives	527:596	The stoichiometry of sulfate with MB among the heparin and derivatives was verified as a non-constant number.
32652759	0	0	theme	Cations	69:75	arg1	Structure					47:55	the Structure	43:55	the Structure of Glycosyl Cations of Protected Galactosides	43:101	The Impact of Leaving Group Anomericity on the Structure of Glycosyl Cations of Protected Galactosides.
32652759	2	1	theme	vibrational	346:356	arg1	spectroscopy					358:369	cryogenic vibrational spectroscopy	336:369	cryogenic vibrational spectroscopy	336:369	Herein, we use cryogenic vibrational spectroscopy and ion mobility-mass spectrometry to study the structure of B-type fragments of protected galactosides.
32652759	4	2	theme	obtained	630:637	arg1	signatures					648:657	The obtained infrared signatures	626:657	The obtained infrared signatures	626:657	The obtained infrared signatures indicate that the investigated fragments exhibit an identical structure, which suggests that there is no anomeric memory in B-type ions of fully protected monosaccharides.
32652759	0	3	theme	Glycosyl	60:67	arg1	Cations					69:75	Glycosyl Cations	60:75	Glycosyl Cations of Protected Galactosides	60:101	The Impact of Leaving Group Anomericity on the Structure of Glycosyl Cations of Protected Galactosides.
32652759	0	4	from	Impact	4:9	arg1	Structure					47:55	the Structure	43:55	the Structure of Glycosyl Cations of Protected Galactosides	43:101	The Impact of Leaving Group Anomericity on the Structure of Glycosyl Cations of Protected Galactosides.
32652759	2	5	theme	cryogenic	336:344	arg1	spectroscopy					358:369	cryogenic vibrational spectroscopy	336:369	cryogenic vibrational spectroscopy	336:369	Herein, we use cryogenic vibrational spectroscopy and ion mobility-mass spectrometry to study the structure of B-type fragments of protected galactosides.
32652759	1	6	theme	anomeric	245:252	arg1	configuration					254:266	their anomeric configuration	239:266	their anomeric configuration	239:266	It has been reported that fragments produced by glycosidic bond breakage in mass spectrometry-based experiments can retain a memory of their anomeric configuration, which has major implications for glycan sequencing.
32652759	0	7	theme	Protected	80:88	arg1	Galactosides					90:101	Protected Galactosides	80:101	Protected Galactosides	80:101	The Impact of Leaving Group Anomericity on the Structure of Glycosyl Cations of Protected Galactosides.
32652759	1	8	theme	configuration	254:266	arg1	memory					229:234	a memory	227:234	a memory	227:234	It has been reported that fragments produced by glycosidic bond breakage in mass spectrometry-based experiments can retain a memory of their anomeric configuration, which has major implications for glycan sequencing.
32652759	4	9	theme	monosaccharides	814:828	arg1	ions					790:793	B-type ions	783:793	B-type ions of fully protected monosaccharides	783:828	The obtained infrared signatures indicate that the investigated fragments exhibit an identical structure, which suggests that there is no anomeric memory in B-type ions of fully protected monosaccharides.
32652759	4	10	theme	infrared	639:646	arg1	signatures					648:657	The obtained infrared signatures	626:657	The obtained infrared signatures	626:657	The obtained infrared signatures indicate that the investigated fragments exhibit an identical structure, which suggests that there is no anomeric memory in B-type ions of fully protected monosaccharides.
32652759	1	11	contain	has	275:277	arg1	memory					229:234	a memory	227:234	a memory	227:234	It has been reported that fragments produced by glycosidic bond breakage in mass spectrometry-based experiments can retain a memory of their anomeric configuration, which has major implications for glycan sequencing.
32652759	1	11	contain	has	275:277	arg2	implications					285:296	major implications	279:296	major implications for glycan sequencing	279:318	It has been reported that fragments produced by glycosidic bond breakage in mass spectrometry-based experiments can retain a memory of their anomeric configuration, which has major implications for glycan sequencing.
32652759	1	12	theme	glycosidic	152:161	arg1	breakage					168:175	glycosidic bond breakage	152:175	glycosidic bond breakage	152:175	It has been reported that fragments produced by glycosidic bond breakage in mass spectrometry-based experiments can retain a memory of their anomeric configuration, which has major implications for glycan sequencing.
32652759	0	13	theme	Galactosides	90:101	arg1	Cations					69:75	Glycosyl Cations	60:75	Glycosyl Cations of Protected Galactosides	60:101	The Impact of Leaving Group Anomericity on the Structure of Glycosyl Cations of Protected Galactosides.
32652759	1	14	theme	bond	163:166	arg1	breakage					168:175	glycosidic bond breakage	152:175	glycosidic bond breakage	152:175	It has been reported that fragments produced by glycosidic bond breakage in mass spectrometry-based experiments can retain a memory of their anomeric configuration, which has major implications for glycan sequencing.
32652759	2	15	theme	protected	452:460	arg1	galactosides					462:473	protected galactosides	452:473	protected galactosides	452:473	Herein, we use cryogenic vibrational spectroscopy and ion mobility-mass spectrometry to study the structure of B-type fragments of protected galactosides.
32652759	3	16	theme	configuration	611:623	arg1	groups					582:587	groups	582:587	groups of different anomeric configuration	582:623	Cationic fragments were generated from glycosyl donors carrying trichloroacetimidate or thioethyl leaving groups of different anomeric configuration.
32652759	3	16	theme	configuration	611:623	arg1	configuration					611:623	different anomeric configuration	592:623	different anomeric configuration	592:623	Cationic fragments were generated from glycosyl donors carrying trichloroacetimidate or thioethyl leaving groups of different anomeric configuration.
32652759	2	17	theme	mobility-mass	379:391	arg1	spectrometry					393:404	ion mobility-mass spectrometry	375:404	ion mobility-mass spectrometry	375:404	Herein, we use cryogenic vibrational spectroscopy and ion mobility-mass spectrometry to study the structure of B-type fragments of protected galactosides.
32652759	2	18	theme	ion	375:377	arg1	spectrometry					393:404	ion mobility-mass spectrometry	375:404	ion mobility-mass spectrometry	375:404	Herein, we use cryogenic vibrational spectroscopy and ion mobility-mass spectrometry to study the structure of B-type fragments of protected galactosides.
32652759	2	19	theme	fragments	439:447	arg1	structure					419:427	the structure	415:427	the structure of B-type fragments of protected galactosides	415:473	Herein, we use cryogenic vibrational spectroscopy and ion mobility-mass spectrometry to study the structure of B-type fragments of protected galactosides.
32652759	3	20	theme	different	592:600	arg1	configuration					611:623	different anomeric configuration	592:623	different anomeric configuration	592:623	Cationic fragments were generated from glycosyl donors carrying trichloroacetimidate or thioethyl leaving groups of different anomeric configuration.
32652759	3	21	theme	Cationic	476:483	arg1	fragments					485:493	Cationic fragments	476:493	Cationic fragments	476:493	Cationic fragments were generated from glycosyl donors carrying trichloroacetimidate or thioethyl leaving groups of different anomeric configuration.
32652759	2	22	theme	B-type	432:437	arg1	fragments					439:447	B-type fragments	432:447	B-type fragments of protected galactosides	432:473	Herein, we use cryogenic vibrational spectroscopy and ion mobility-mass spectrometry to study the structure of B-type fragments of protected galactosides.
32652759	1	23	theme	major	279:283	arg1	implications					285:296	major implications	279:296	major implications for glycan sequencing	279:318	It has been reported that fragments produced by glycosidic bond breakage in mass spectrometry-based experiments can retain a memory of their anomeric configuration, which has major implications for glycan sequencing.
32652759	3	24	theme	anomeric	602:609	arg1	configuration					611:623	different anomeric configuration	592:623	different anomeric configuration	592:623	Cationic fragments were generated from glycosyl donors carrying trichloroacetimidate or thioethyl leaving groups of different anomeric configuration.
32652759	1	25	theme	spectrometry-based	185:202	arg1	experiments					204:214	mass spectrometry-based experiments	180:214	mass spectrometry-based experiments	180:214	It has been reported that fragments produced by glycosidic bond breakage in mass spectrometry-based experiments can retain a memory of their anomeric configuration, which has major implications for glycan sequencing.
32652759	0	26	theme	Anomericity	28:38	arg1	Impact					4:9	The Impact	0:9	The Impact of Leaving Group Anomericity on the Structure of Glycosyl Cations of Protected Galactosides.	0:102	The Impact of Leaving Group Anomericity on the Structure of Glycosyl Cations of Protected Galactosides.
32652759	4	27	theme	anomeric	764:771	arg1	memory					773:778	no anomeric memory	761:778	no anomeric memory in B-type ions of fully protected monosaccharides	761:828	The obtained infrared signatures indicate that the investigated fragments exhibit an identical structure, which suggests that there is no anomeric memory in B-type ions of fully protected monosaccharides.
32652759	4	28	theme	identical	711:719	arg1	structure					721:729	an identical structure	708:729	an identical structure	708:729	The obtained infrared signatures indicate that the investigated fragments exhibit an identical structure, which suggests that there is no anomeric memory in B-type ions of fully protected monosaccharides.
32652759	0	29	theme	Group	22:26	arg1	Anomericity					28:38	Leaving Group Anomericity	14:38	Leaving Group Anomericity	14:38	The Impact of Leaving Group Anomericity on the Structure of Glycosyl Cations of Protected Galactosides.
32652759	0	30	theme	Leaving	14:20	arg1	Anomericity					28:38	Leaving Group Anomericity	14:38	Leaving Group Anomericity	14:38	The Impact of Leaving Group Anomericity on the Structure of Glycosyl Cations of Protected Galactosides.
32652759	1	31	theme	glycan	302:307	arg1	sequencing					309:318	glycan sequencing	302:318	glycan sequencing	302:318	It has been reported that fragments produced by glycosidic bond breakage in mass spectrometry-based experiments can retain a memory of their anomeric configuration, which has major implications for glycan sequencing.
32652759	2	32	theme	galactosides	462:473	arg1	fragments					439:447	B-type fragments	432:447	B-type fragments of protected galactosides	432:473	Herein, we use cryogenic vibrational spectroscopy and ion mobility-mass spectrometry to study the structure of B-type fragments of protected galactosides.
32652759	3	33	theme	glycosyl	515:522	arg1	donors					524:529	glycosyl donors	515:529	glycosyl donors carrying trichloroacetimidate or thioethyl leaving groups of different anomeric configuration	515:623	Cationic fragments were generated from glycosyl donors carrying trichloroacetimidate or thioethyl leaving groups of different anomeric configuration.
32652759	4	34	theme	B-type	783:788	arg1	ions					790:793	B-type ions	783:793	B-type ions of fully protected monosaccharides	783:828	The obtained infrared signatures indicate that the investigated fragments exhibit an identical structure, which suggests that there is no anomeric memory in B-type ions of fully protected monosaccharides.
32652759	4	35	theme	protected	804:812	arg1	monosaccharides					814:828	fully protected monosaccharides	798:828	fully protected monosaccharides	798:828	The obtained infrared signatures indicate that the investigated fragments exhibit an identical structure, which suggests that there is no anomeric memory in B-type ions of fully protected monosaccharides.
32652759	4	36	from	memory	773:778	arg1	ions					790:793	B-type ions	783:793	B-type ions of fully protected monosaccharides	783:828	The obtained infrared signatures indicate that the investigated fragments exhibit an identical structure, which suggests that there is no anomeric memory in B-type ions of fully protected monosaccharides.
32652759	4	37	theme	investigated	677:688	arg1	fragments					690:698	the investigated fragments	673:698	the investigated fragments	673:698	The obtained infrared signatures indicate that the investigated fragments exhibit an identical structure, which suggests that there is no anomeric memory in B-type ions of fully protected monosaccharides.
32652759	3	38	contain	carrying	531:538	arg2	thioethyl					564:572	thioethyl	564:572	thioethyl	564:572	Cationic fragments were generated from glycosyl donors carrying trichloroacetimidate or thioethyl leaving groups of different anomeric configuration.
32652759	3	38	contain	carrying	531:538	arg2	trichloroacetimidate					540:559	trichloroacetimidate	540:559	trichloroacetimidate	540:559	Cationic fragments were generated from glycosyl donors carrying trichloroacetimidate or thioethyl leaving groups of different anomeric configuration.
32652759	3	38	contain	carrying	531:538	arg1	donors					524:529	glycosyl donors	515:529	glycosyl donors carrying trichloroacetimidate or thioethyl leaving groups of different anomeric configuration	515:623	Cationic fragments were generated from glycosyl donors carrying trichloroacetimidate or thioethyl leaving groups of different anomeric configuration.
32284273	8	0	theme	efficacious	1659:1669	arg1	vaccines					1681:1688	more efficacious VLP-based vaccines	1654:1688	more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering	1654:1773	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	6	1	theme	VLP	1292:1294	arg1	derivatives					1296:1306	different VLP derivatives	1282:1306	different VLP derivatives	1282:1306	To assess whether hyper-glycosylated VLPs induce an enhanced immune response in the presence of the adjuvant aluminum hydroxide, the anti-HBV surface antigen (anti-HBsAgS) antibody response was monitored in BALB/c mice, subcutaneously injected with different VLP derivatives.
32284273	8	2	theme	carrier	1734:1740	arg1	platforms					1742:1750	hepatitis B and improved HBsAgS VLP carrier platforms	1698:1750	hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering	1698:1773	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	8	3	gly	hyper-glycosylated	1444:1461	arg1	VLPs					1463:1466	hyper-glycosylated VLPs	1444:1466	hyper-glycosylated VLPs	1444:1466	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	6	4	theme	surface	1175:1181	arg1	anti-HBsAgS					1192:1202	anti-HBsAgS	1192:1202	anti-HBsAgS	1192:1202	To assess whether hyper-glycosylated VLPs induce an enhanced immune response in the presence of the adjuvant aluminum hydroxide, the anti-HBV surface antigen (anti-HBsAgS) antibody response was monitored in BALB/c mice, subcutaneously injected with different VLP derivatives.
32284273	6	4	theme	surface	1175:1181	arg1	antigen					1183:1189	anti-HBV surface antigen	1166:1189	the anti-HBV surface antigen (anti-HBsAgS) antibody response	1162:1221	To assess whether hyper-glycosylated VLPs induce an enhanced immune response in the presence of the adjuvant aluminum hydroxide, the anti-HBV surface antigen (anti-HBsAgS) antibody response was monitored in BALB/c mice, subcutaneously injected with different VLP derivatives.
32284273	2	5	theme	immunogenic	349:359	arg1	VLPs					248:251	VLPs	248:251	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV)	248:339	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	2	5	theme	immunogenic	349:359	arg1	components					361:370	the immunogenic components	345:370	the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant	345:453	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	4	6	theme	N-glycan	871:878	arg1	structures					880:889	N-glycan structures	871:889	N-glycan structures	871:889	Comparative mass spectrometry-based N-glycan profiling, gel electrophoresis, and immunoassays demonstrated that WT and hyper-glycosylated HBsAgS VLPs contain the same type and distribution of N-glycan structures, but the latter shows a higher glycan abundance per protein mass.
32284273	1	7	theme	delivery	196:203	arg1	platforms					205:213	delivery platforms	196:213	delivery platforms	196:213	Virus-like particles (VLP) represent biological platforms for the development of novel products such as vaccines and delivery platforms for foreign antigenic sequences.
32284273	7	8	gly	hyper-glycosylated	1350:1367	arg1	VLPs					1369:1372	hyper-glycosylated VLPs	1350:1372	hyper-glycosylated VLPs	1350:1372	In the absence and presence of adjuvant, hyper-glycosylated VLPs showed an enhanced immunogenicity compared to WT VLPs.
32284273	6	9	theme	hydroxide	1151:1159	arg1	presence					1117:1124	the presence	1113:1124	the presence of the adjuvant aluminum hydroxide	1113:1159	To assess whether hyper-glycosylated VLPs induce an enhanced immune response in the presence of the adjuvant aluminum hydroxide, the anti-HBV surface antigen (anti-HBsAgS) antibody response was monitored in BALB/c mice, subcutaneously injected with different VLP derivatives.
32284273	4	10	theme	protein	943:949	arg1	mass					951:954	protein mass	943:954	protein mass	943:954	Comparative mass spectrometry-based N-glycan profiling, gel electrophoresis, and immunoassays demonstrated that WT and hyper-glycosylated HBsAgS VLPs contain the same type and distribution of N-glycan structures, but the latter shows a higher glycan abundance per protein mass.
32284273	8	11	theme	VLPs	1463:1466	arg1	ability					1433:1439	The ability	1429:1439	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs	1429:1614	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	6	12	theme	BALB/c	1240:1245	arg1	mice					1247:1250	BALB/c mice	1240:1250	BALB/c mice	1240:1250	To assess whether hyper-glycosylated VLPs induce an enhanced immune response in the presence of the adjuvant aluminum hydroxide, the anti-HBV surface antigen (anti-HBsAgS) antibody response was monitored in BALB/c mice, subcutaneously injected with different VLP derivatives.
32284273	3	13	theme	wild	608:611	arg1	WT					619:620	WT	619:620	WT	619:620	Herein, we report that glycoengineering of N-glycosylated HBsAgS to generate hyper-glycosylated VLPs display an enhanced immunogenicity relative to the wild type (WT) HBsAgS VLPs when expressed in FreeStyle HEK 293F cells.
32284273	3	13	theme	wild	608:611	arg1	type					613:616	wild type	608:616	the wild type (WT) HBsAgS VLPs	604:633	Herein, we report that glycoengineering of N-glycosylated HBsAgS to generate hyper-glycosylated VLPs display an enhanced immunogenicity relative to the wild type (WT) HBsAgS VLPs when expressed in FreeStyle HEK 293F cells.
32284273	1	14	theme	antigenic	227:235	arg1	sequences					237:245	foreign antigenic sequences	219:245	foreign antigenic sequences	219:245	Virus-like particles (VLP) represent biological platforms for the development of novel products such as vaccines and delivery platforms for foreign antigenic sequences.
32284273	4	15	theme	N-glycan	715:722	arg1	profiling					724:732	Comparative mass spectrometry-based N-glycan profiling	679:732	Comparative mass spectrometry-based N-glycan profiling	679:732	Comparative mass spectrometry-based N-glycan profiling, gel electrophoresis, and immunoassays demonstrated that WT and hyper-glycosylated HBsAgS VLPs contain the same type and distribution of N-glycan structures, but the latter shows a higher glycan abundance per protein mass.
32284273	4	16	theme	higher	915:920	arg1	abundance					929:937	a higher glycan abundance	913:937	a higher glycan abundance per protein mass	913:954	Comparative mass spectrometry-based N-glycan profiling, gel electrophoresis, and immunoassays demonstrated that WT and hyper-glycosylated HBsAgS VLPs contain the same type and distribution of N-glycan structures, but the latter shows a higher glycan abundance per protein mass.
32284273	8	17	theme	anti-HBsAgS	1486:1496	arg1	responses					1505:1513	potent anti-HBsAgS immune responses	1479:1513	potent anti-HBsAgS immune responses	1479:1513	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	2	18	theme	small	269:273	arg1	HBsAgS					292:297	HBsAgS	292:297	HBsAgS	292:297	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	2	18	theme	small	269:273	arg1	antigen					283:289	the small surface antigen	265:289	the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV)	265:339	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	1	19	theme	novel	160:164	arg1	products					166:173	novel products	160:173	novel products such as vaccines and delivery platforms for foreign antigenic sequences	160:245	Virus-like particles (VLP) represent biological platforms for the development of novel products such as vaccines and delivery platforms for foreign antigenic sequences.
32284273	1	19	theme	novel	160:164	arg1	vaccines					183:190	vaccines	183:190	vaccines	183:190	Virus-like particles (VLP) represent biological platforms for the development of novel products such as vaccines and delivery platforms for foreign antigenic sequences.
32284273	1	19	theme	novel	160:164	arg1	platforms					205:213	delivery platforms	196:213	delivery platforms	196:213	Virus-like particles (VLP) represent biological platforms for the development of novel products such as vaccines and delivery platforms for foreign antigenic sequences.
32284273	8	20	with	VLPs	1611:1614	arg1	level					1555:1559	a native N-glycan level	1537:1559	a native N-glycan level	1537:1559	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	1	21	theme	biological	116:125	arg1	platforms					127:135	biological platforms	116:135	biological platforms for the development of novel products such as vaccines and delivery platforms for foreign antigenic sequences	116:245	Virus-like particles (VLP) represent biological platforms for the development of novel products such as vaccines and delivery platforms for foreign antigenic sequences.
32284273	4	22	contain	contain	829:835	arg2	type					846:849	the same type	837:849	the same type	837:849	Comparative mass spectrometry-based N-glycan profiling, gel electrophoresis, and immunoassays demonstrated that WT and hyper-glycosylated HBsAgS VLPs contain the same type and distribution of N-glycan structures, but the latter shows a higher glycan abundance per protein mass.
32284273	4	22	contain	contain	829:835	arg1	VLPs					824:827	WT and hyper-glycosylated HBsAgS VLPs	791:827	WT and hyper-glycosylated HBsAgS VLPs	791:827	Comparative mass spectrometry-based N-glycan profiling, gel electrophoresis, and immunoassays demonstrated that WT and hyper-glycosylated HBsAgS VLPs contain the same type and distribution of N-glycan structures, but the latter shows a higher glycan abundance per protein mass.
32284273	4	22	contain	contain	829:835	arg2	distribution					855:866	distribution	855:866	distribution	855:866	Comparative mass spectrometry-based N-glycan profiling, gel electrophoresis, and immunoassays demonstrated that WT and hyper-glycosylated HBsAgS VLPs contain the same type and distribution of N-glycan structures, but the latter shows a higher glycan abundance per protein mass.
32284273	1	23	theme	Virus-like	79:88	arg1	particles					90:98	Virus-like particles	79:98	Virus-like particles (VLP)	79:104	Virus-like particles (VLP) represent biological platforms for the development of novel products such as vaccines and delivery platforms for foreign antigenic sequences.
32284273	1	23	theme	Virus-like	79:88	arg1	VLP					101:103	VLP	101:103	VLP	101:103	Virus-like particles (VLP) represent biological platforms for the development of novel products such as vaccines and delivery platforms for foreign antigenic sequences.
32284273	8	24	theme	native	1539:1544	arg1	level					1555:1559	a native N-glycan level	1537:1559	a native N-glycan level	1537:1559	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	4	25	theme	WT	791:792	arg1	VLPs					824:827	WT and hyper-glycosylated HBsAgS VLPs	791:827	WT and hyper-glycosylated HBsAgS VLPs	791:827	Comparative mass spectrometry-based N-glycan profiling, gel electrophoresis, and immunoassays demonstrated that WT and hyper-glycosylated HBsAgS VLPs contain the same type and distribution of N-glycan structures, but the latter shows a higher glycan abundance per protein mass.
32284273	2	26	theme	licensed	377:384	arg1	vaccine					400:406	a licensed, preventative vaccine	375:406	vaccine	400:406	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	5	27	theme	modified	988:995	arg1	VLPs					997:1000	the modified VLPs	984:1000	the modified VLPs	984:1000	The antigenic integrity of the modified VLPs was also shown to be retained.
32284273	7	28	theme	adjuvant	1340:1347	arg1	presence					1328:1335	presence	1328:1335	presence	1328:1335	In the absence and presence of adjuvant, hyper-glycosylated VLPs showed an enhanced immunogenicity compared to WT VLPs.
32284273	7	28	theme	adjuvant	1340:1347	arg1	absence					1316:1322	absence	1316:1322	absence	1316:1322	In the absence and presence of adjuvant, hyper-glycosylated VLPs showed an enhanced immunogenicity compared to WT VLPs.
32284273	8	29	with	VLPs	1527:1530	arg1	level					1555:1559	a native N-glycan level	1537:1559	a native N-glycan level	1537:1559	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	0	30	theme	Glycoengineered	0:14	arg1	particles					39:47	Glycoengineered hepatitis B virus-like particles	0:47	Glycoengineered hepatitis B virus-like particles with	0:52	Glycoengineered hepatitis B virus-like particles with enhanced immunogenicity.
32284273	0	31	theme	B	26:26	arg1	particles					39:47	Glycoengineered hepatitis B virus-like particles	0:47	Glycoengineered hepatitis B virus-like particles with	0:52	Glycoengineered hepatitis B virus-like particles with enhanced immunogenicity.
32284273	5	32	theme	antigenic	961:969	arg1	integrity					971:979	The antigenic integrity	957:979	The antigenic integrity of the modified VLPs	957:1000	The antigenic integrity of the modified VLPs was also shown to be retained.
32284273	8	33	theme	non-glycosylated	1572:1587	arg1	VLPs					1611:1614	non-glycosylated, yeast-derived HBsAgS VLPs	1572:1614	VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs	1527:1614	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	2	34	theme	preventative	387:398	arg1	vaccine					400:406	a licensed, preventative vaccine	375:406	vaccine	400:406	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	4	35	gly	hyper-glycosylated	798:815	arg1	HBsAgS					817:822	hyper-glycosylated HBsAgS	798:822	hyper-glycosylated HBsAgS	798:822	Comparative mass spectrometry-based N-glycan profiling, gel electrophoresis, and immunoassays demonstrated that WT and hyper-glycosylated HBsAgS VLPs contain the same type and distribution of N-glycan structures, but the latter shows a higher glycan abundance per protein mass.
32284273	3	36	theme	293F	667:670	arg1	cells					672:676	FreeStyle HEK 293F cells	653:676	FreeStyle HEK 293F cells	653:676	Herein, we report that glycoengineering of N-glycosylated HBsAgS to generate hyper-glycosylated VLPs display an enhanced immunogenicity relative to the wild type (WT) HBsAgS VLPs when expressed in FreeStyle HEK 293F cells.
32284273	4	37	theme	hyper-glycosylated	798:815	arg1	HBsAgS					817:822	hyper-glycosylated HBsAgS	798:822	hyper-glycosylated HBsAgS	798:822	Comparative mass spectrometry-based N-glycan profiling, gel electrophoresis, and immunoassays demonstrated that WT and hyper-glycosylated HBsAgS VLPs contain the same type and distribution of N-glycan structures, but the latter shows a higher glycan abundance per protein mass.
32284273	8	38	theme	exciting	1622:1629	arg1	avenues					1631:1637	exciting avenues	1622:1637	exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering	1622:1773	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	3	39	theme	FreeStyle	653:661	arg1	cells					672:676	FreeStyle HEK 293F cells	653:676	FreeStyle HEK 293F cells	653:676	Herein, we report that glycoengineering of N-glycosylated HBsAgS to generate hyper-glycosylated VLPs display an enhanced immunogenicity relative to the wild type (WT) HBsAgS VLPs when expressed in FreeStyle HEK 293F cells.
32284273	8	40	theme	hyper-glycosylated	1444:1461	arg1	VLPs					1463:1466	hyper-glycosylated VLPs	1444:1466	hyper-glycosylated VLPs	1444:1466	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	3	41	theme	N-glycosylated	499:512	arg1	HBsAgS					514:519	N-glycosylated HBsAgS	499:519	N-glycosylated HBsAgS	499:519	Herein, we report that glycoengineering of N-glycosylated HBsAgS to generate hyper-glycosylated VLPs display an enhanced immunogenicity relative to the wild type (WT) HBsAgS VLPs when expressed in FreeStyle HEK 293F cells.
32284273	8	42	theme	B	1708:1708	arg1	platforms					1742:1750	hepatitis B and improved HBsAgS VLP carrier platforms	1698:1750	hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering	1698:1773	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	7	43	dep	absence	1316:1322	arg1	the					1312:1314	the	1312:1314	the	1312:1314	In the absence and presence of adjuvant, hyper-glycosylated VLPs showed an enhanced immunogenicity compared to WT VLPs.
32284273	8	44	theme	improved	1714:1721	arg1	HBsAgS					1723:1728	improved HBsAgS	1714:1728	improved HBsAgS	1714:1728	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	4	45	theme	structures	880:889	arg1	distribution					855:866	distribution	855:866	distribution	855:866	Comparative mass spectrometry-based N-glycan profiling, gel electrophoresis, and immunoassays demonstrated that WT and hyper-glycosylated HBsAgS VLPs contain the same type and distribution of N-glycan structures, but the latter shows a higher glycan abundance per protein mass.
32284273	4	45	theme	structures	880:889	arg1	type					846:849	the same type	837:849	the same type	837:849	Comparative mass spectrometry-based N-glycan profiling, gel electrophoresis, and immunoassays demonstrated that WT and hyper-glycosylated HBsAgS VLPs contain the same type and distribution of N-glycan structures, but the latter shows a higher glycan abundance per protein mass.
32284273	6	46	theme	immune	1094:1099	arg1	response					1101:1108	an enhanced immune response	1082:1108	an enhanced immune response	1082:1108	To assess whether hyper-glycosylated VLPs induce an enhanced immune response in the presence of the adjuvant aluminum hydroxide, the anti-HBV surface antigen (anti-HBsAgS) antibody response was monitored in BALB/c mice, subcutaneously injected with different VLP derivatives.
32284273	6	47	theme	different	1282:1290	arg1	derivatives					1296:1306	different VLP derivatives	1282:1306	different VLP derivatives	1282:1306	To assess whether hyper-glycosylated VLPs induce an enhanced immune response in the presence of the adjuvant aluminum hydroxide, the anti-HBV surface antigen (anti-HBsAgS) antibody response was monitored in BALB/c mice, subcutaneously injected with different VLP derivatives.
32284273	2	48	contain	contains	415:422	arg2	hydroxide					433:441	aluminum hydroxide	424:441	aluminum hydroxide	424:441	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	2	48	contain	contains	415:422	arg1	vaccine					400:406	a licensed, preventative vaccine	375:406	vaccine	400:406	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	3	49	theme	hyper-glycosylated	533:550	arg1	VLPs					552:555	hyper-glycosylated VLPs	533:555	hyper-glycosylated VLPs	533:555	Herein, we report that glycoengineering of N-glycosylated HBsAgS to generate hyper-glycosylated VLPs display an enhanced immunogenicity relative to the wild type (WT) HBsAgS VLPs when expressed in FreeStyle HEK 293F cells.
32284273	8	50	theme	VLP	1730:1732	arg1	platforms					1742:1750	hepatitis B and improved HBsAgS VLP carrier platforms	1698:1750	hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering	1698:1773	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	2	51	theme	hepatitis	317:325	arg1	HBV					336:338	HBV	336:338	HBV	336:338	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	2	51	theme	hepatitis	317:325	arg1	virus					329:333	the hepatitis B virus	313:333	the hepatitis B virus (HBV)	313:339	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	6	52	theme	antigen	1183:1189	arg1	response					1214:1221	the anti-HBV surface antigen (anti-HBsAgS) antibody response	1162:1221	the anti-HBV surface antigen (anti-HBsAgS) antibody response	1162:1221	To assess whether hyper-glycosylated VLPs induce an enhanced immune response in the presence of the adjuvant aluminum hydroxide, the anti-HBV surface antigen (anti-HBsAgS) antibody response was monitored in BALB/c mice, subcutaneously injected with different VLP derivatives.
32284273	8	53	theme	VLP-based	1671:1679	arg1	vaccines					1681:1688	more efficacious VLP-based vaccines	1654:1688	more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering	1654:1773	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	6	54	theme	anti-HBV	1166:1173	arg1	anti-HBsAgS					1192:1202	anti-HBsAgS	1192:1202	anti-HBsAgS	1192:1202	To assess whether hyper-glycosylated VLPs induce an enhanced immune response in the presence of the adjuvant aluminum hydroxide, the anti-HBV surface antigen (anti-HBsAgS) antibody response was monitored in BALB/c mice, subcutaneously injected with different VLP derivatives.
32284273	6	54	theme	anti-HBV	1166:1173	arg1	antigen					1183:1189	anti-HBV surface antigen	1166:1189	the anti-HBV surface antigen (anti-HBsAgS) antibody response	1162:1221	To assess whether hyper-glycosylated VLPs induce an enhanced immune response in the presence of the adjuvant aluminum hydroxide, the anti-HBV surface antigen (anti-HBsAgS) antibody response was monitored in BALB/c mice, subcutaneously injected with different VLP derivatives.
32284273	3	55	theme	enhanced	568:575	arg1	immunogenicity					577:590	an enhanced immunogenicity	565:590	an enhanced immunogenicity relative to the wild type (WT) HBsAgS VLPs	565:633	Herein, we report that glycoengineering of N-glycosylated HBsAgS to generate hyper-glycosylated VLPs display an enhanced immunogenicity relative to the wild type (WT) HBsAgS VLPs when expressed in FreeStyle HEK 293F cells.
32284273	2	56	attach	derived	300:306	arg2	antigen					283:289	the small surface antigen	265:289	the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV)	265:339	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	2	56	attach	derived	300:306	arg1	HBV					336:338	HBV	336:338	HBV	336:338	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	2	56	attach	derived	300:306	arg1	virus					329:333	the hepatitis B virus	313:333	the hepatitis B virus (HBV)	313:339	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	2	56	attach	derived	300:306	arg2	HBsAgS					292:297	HBsAgS	292:297	HBsAgS	292:297	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	4	57	theme	glycan	922:927	arg1	abundance					929:937	a higher glycan abundance	913:937	a higher glycan abundance per protein mass	913:954	Comparative mass spectrometry-based N-glycan profiling, gel electrophoresis, and immunoassays demonstrated that WT and hyper-glycosylated HBsAgS VLPs contain the same type and distribution of N-glycan structures, but the latter shows a higher glycan abundance per protein mass.
32284273	7	58	theme	WT	1420:1421	arg1	VLPs					1423:1426	WT VLPs	1420:1426	WT VLPs	1420:1426	In the absence and presence of adjuvant, hyper-glycosylated VLPs showed an enhanced immunogenicity compared to WT VLPs.
32284273	1	59	theme	foreign	219:225	arg1	sequences					237:245	foreign antigenic sequences	219:245	foreign antigenic sequences	219:245	Virus-like particles (VLP) represent biological platforms for the development of novel products such as vaccines and delivery platforms for foreign antigenic sequences.
32284273	8	60	theme	potent	1479:1484	arg1	responses					1505:1513	potent anti-HBsAgS immune responses	1479:1513	potent anti-HBsAgS immune responses	1479:1513	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	3	61	theme	relative	592:599	arg1	immunogenicity					577:590	an enhanced immunogenicity	565:590	an enhanced immunogenicity relative to the wild type (WT) HBsAgS VLPs	565:633	Herein, we report that glycoengineering of N-glycosylated HBsAgS to generate hyper-glycosylated VLPs display an enhanced immunogenicity relative to the wild type (WT) HBsAgS VLPs when expressed in FreeStyle HEK 293F cells.
32284273	8	62	theme	immune	1498:1503	arg1	responses					1505:1513	potent anti-HBsAgS immune responses	1479:1513	potent anti-HBsAgS immune responses	1479:1513	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	7	63	theme	enhanced	1384:1391	arg1	immunogenicity					1393:1406	an enhanced immunogenicity	1381:1406	an enhanced immunogenicity	1381:1406	In the absence and presence of adjuvant, hyper-glycosylated VLPs showed an enhanced immunogenicity compared to WT VLPs.
32284273	4	64	theme	spectrometry-based	696:713	arg1	profiling					724:732	Comparative mass spectrometry-based N-glycan profiling	679:732	Comparative mass spectrometry-based N-glycan profiling	679:732	Comparative mass spectrometry-based N-glycan profiling, gel electrophoresis, and immunoassays demonstrated that WT and hyper-glycosylated HBsAgS VLPs contain the same type and distribution of N-glycan structures, but the latter shows a higher glycan abundance per protein mass.
32284273	8	65	dep	non-glycosylated	1572:1587	arg1	yeast-derived					1590:1602	yeast-derived	1590:1602	yeast-derived	1590:1602	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	2	66	theme	surface	275:281	arg1	HBsAgS					292:297	HBsAgS	292:297	HBsAgS	292:297	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	2	66	theme	surface	275:281	arg1	antigen					283:289	the small surface antigen	265:289	the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV)	265:339	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	8	67	dep	ability	1433:1439	arg1	promote					1471:1477	promote	1471:1477	to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs	1468:1614	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	6	68	theme	antibody	1205:1212	arg1	response					1214:1221	the anti-HBV surface antigen (anti-HBsAgS) antibody response	1162:1221	the anti-HBV surface antigen (anti-HBsAgS) antibody response	1162:1221	To assess whether hyper-glycosylated VLPs induce an enhanced immune response in the presence of the adjuvant aluminum hydroxide, the anti-HBV surface antigen (anti-HBsAgS) antibody response was monitored in BALB/c mice, subcutaneously injected with different VLP derivatives.
32284273	3	69	theme	type	613:616	arg1	VLPs					630:633	the wild type (WT) HBsAgS VLPs	604:633	the wild type (WT) HBsAgS VLPs	604:633	Herein, we report that glycoengineering of N-glycosylated HBsAgS to generate hyper-glycosylated VLPs display an enhanced immunogenicity relative to the wild type (WT) HBsAgS VLPs when expressed in FreeStyle HEK 293F cells.
32284273	7	70	theme	hyper-glycosylated	1350:1367	arg1	VLPs					1369:1372	hyper-glycosylated VLPs	1350:1372	hyper-glycosylated VLPs	1350:1372	In the absence and presence of adjuvant, hyper-glycosylated VLPs showed an enhanced immunogenicity compared to WT VLPs.
32284273	3	71	gly	hyper-glycosylated	533:550	arg1	VLPs					552:555	hyper-glycosylated VLPs	533:555	hyper-glycosylated VLPs	533:555	Herein, we report that glycoengineering of N-glycosylated HBsAgS to generate hyper-glycosylated VLPs display an enhanced immunogenicity relative to the wild type (WT) HBsAgS VLPs when expressed in FreeStyle HEK 293F cells.
32284273	1	72	theme	products	166:173	arg1	development					145:155	the development	141:155	the development of novel products such as vaccines and delivery platforms for foreign antigenic sequences	141:245	Virus-like particles (VLP) represent biological platforms for the development of novel products such as vaccines and delivery platforms for foreign antigenic sequences.
32284273	4	73	theme	Comparative	679:689	arg1	profiling					724:732	Comparative mass spectrometry-based N-glycan profiling	679:732	Comparative mass spectrometry-based N-glycan profiling	679:732	Comparative mass spectrometry-based N-glycan profiling, gel electrophoresis, and immunoassays demonstrated that WT and hyper-glycosylated HBsAgS VLPs contain the same type and distribution of N-glycan structures, but the latter shows a higher glycan abundance per protein mass.
32284273	6	74	theme	aluminum	1142:1149	arg1	hydroxide					1151:1159	the adjuvant aluminum hydroxide	1129:1159	the adjuvant aluminum hydroxide	1129:1159	To assess whether hyper-glycosylated VLPs induce an enhanced immune response in the presence of the adjuvant aluminum hydroxide, the anti-HBV surface antigen (anti-HBsAgS) antibody response was monitored in BALB/c mice, subcutaneously injected with different VLP derivatives.
32284273	8	75	theme	N-glycan	1546:1553	arg1	level					1555:1559	a native N-glycan level	1537:1559	a native N-glycan level	1537:1559	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	3	76	gly	N-glycosylated	499:512	arg1	HBsAgS					514:519	N-glycosylated HBsAgS	499:519	N-glycosylated HBsAgS	499:519	Herein, we report that glycoengineering of N-glycosylated HBsAgS to generate hyper-glycosylated VLPs display an enhanced immunogenicity relative to the wild type (WT) HBsAgS VLPs when expressed in FreeStyle HEK 293F cells.
32284273	8	77	gly	non-glycosylated	1572:1587	arg1	VLPs					1611:1614	non-glycosylated, yeast-derived HBsAgS VLPs	1572:1614	VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs	1527:1614	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	6	78	theme	enhanced	1085:1092	arg1	response					1101:1108	an enhanced immune response	1082:1108	an enhanced immune response	1082:1108	To assess whether hyper-glycosylated VLPs induce an enhanced immune response in the presence of the adjuvant aluminum hydroxide, the anti-HBV surface antigen (anti-HBsAgS) antibody response was monitored in BALB/c mice, subcutaneously injected with different VLP derivatives.
32284273	0	79	theme	hepatitis	16:24	arg1	particles					39:47	Glycoengineered hepatitis B virus-like particles	0:47	Glycoengineered hepatitis B virus-like particles with	0:52	Glycoengineered hepatitis B virus-like particles with enhanced immunogenicity.
32284273	3	80	dep	glycoengineering	479:494	arg1	generate					524:531	generate	524:531	to generate hyper-glycosylated VLPs	521:555	Herein, we report that glycoengineering of N-glycosylated HBsAgS to generate hyper-glycosylated VLPs display an enhanced immunogenicity relative to the wild type (WT) HBsAgS VLPs when expressed in FreeStyle HEK 293F cells.
32284273	2	81	theme	aluminum	424:431	arg1	hydroxide					433:441	aluminum hydroxide	424:441	aluminum hydroxide	424:441	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	4	82	theme	gel	735:737	arg1	electrophoresis					739:753	gel electrophoresis	735:753	gel electrophoresis	735:753	Comparative mass spectrometry-based N-glycan profiling, gel electrophoresis, and immunoassays demonstrated that WT and hyper-glycosylated HBsAgS VLPs contain the same type and distribution of N-glycan structures, but the latter shows a higher glycan abundance per protein mass.
32284273	0	83	theme	virus-like	28:37	arg1	particles					39:47	Glycoengineered hepatitis B virus-like particles	0:47	Glycoengineered hepatitis B virus-like particles with	0:52	Glycoengineered hepatitis B virus-like particles with enhanced immunogenicity.
32284273	6	84	theme	hyper-glycosylated	1051:1068	arg1	VLPs					1070:1073	hyper-glycosylated VLPs	1051:1073	hyper-glycosylated VLPs	1051:1073	To assess whether hyper-glycosylated VLPs induce an enhanced immune response in the presence of the adjuvant aluminum hydroxide, the anti-HBV surface antigen (anti-HBsAgS) antibody response was monitored in BALB/c mice, subcutaneously injected with different VLP derivatives.
32284273	6	85	gly	hyper-glycosylated	1051:1068	arg1	VLPs					1070:1073	hyper-glycosylated VLPs	1051:1073	hyper-glycosylated VLPs	1051:1073	To assess whether hyper-glycosylated VLPs induce an enhanced immune response in the presence of the adjuvant aluminum hydroxide, the anti-HBV surface antigen (anti-HBsAgS) antibody response was monitored in BALB/c mice, subcutaneously injected with different VLP derivatives.
32284273	2	86	theme	vaccine	400:406	arg1	VLPs					248:251	VLPs	248:251	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV)	248:339	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	2	86	theme	vaccine	400:406	arg1	components					361:370	the immunogenic components	345:370	the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant	345:453	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	8	87	theme	HBsAgS	1604:1609	arg1	VLPs					1611:1614	non-glycosylated, yeast-derived HBsAgS VLPs	1572:1614	VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs	1527:1614	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	3	88	theme	HBsAgS	623:628	arg1	VLPs					630:633	the wild type (WT) HBsAgS VLPs	604:633	the wild type (WT) HBsAgS VLPs	604:633	Herein, we report that glycoengineering of N-glycosylated HBsAgS to generate hyper-glycosylated VLPs display an enhanced immunogenicity relative to the wild type (WT) HBsAgS VLPs when expressed in FreeStyle HEK 293F cells.
32284273	6	89	theme	adjuvant	1133:1140	arg1	hydroxide					1151:1159	the adjuvant aluminum hydroxide	1129:1159	the adjuvant aluminum hydroxide	1129:1159	To assess whether hyper-glycosylated VLPs induce an enhanced immune response in the presence of the adjuvant aluminum hydroxide, the anti-HBV surface antigen (anti-HBsAgS) antibody response was monitored in BALB/c mice, subcutaneously injected with different VLP derivatives.
32284273	3	90	theme	HEK	663:665	arg1	cells					672:676	FreeStyle HEK 293F cells	653:676	FreeStyle HEK 293F cells	653:676	Herein, we report that glycoengineering of N-glycosylated HBsAgS to generate hyper-glycosylated VLPs display an enhanced immunogenicity relative to the wild type (WT) HBsAgS VLPs when expressed in FreeStyle HEK 293F cells.
32284273	4	91	theme	same	841:844	arg1	type					846:849	the same type	837:849	the same type	837:849	Comparative mass spectrometry-based N-glycan profiling, gel electrophoresis, and immunoassays demonstrated that WT and hyper-glycosylated HBsAgS VLPs contain the same type and distribution of N-glycan structures, but the latter shows a higher glycan abundance per protein mass.
32284273	8	92	theme	hepatitis	1698:1706	arg1	B					1708:1708	hepatitis B	1698:1708	hepatitis B	1698:1708	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32284273	5	93	theme	VLPs	997:1000	arg1	integrity					971:979	The antigenic integrity	957:979	The antigenic integrity of the modified VLPs	957:1000	The antigenic integrity of the modified VLPs was also shown to be retained.
32284273	2	94	theme	B	327:327	arg1	HBV					336:338	HBV	336:338	HBV	336:338	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	2	94	theme	B	327:327	arg1	virus					329:333	the hepatitis B virus	313:333	the hepatitis B virus (HBV)	313:339	VLPs composed of the small surface antigen (HBsAgS) derived from the hepatitis B virus (HBV) are the immunogenic components of a licensed, preventative vaccine, which contains aluminum hydroxide as adjuvant.
32284273	4	95	theme	HBsAgS	817:822	arg1	VLPs					824:827	WT and hyper-glycosylated HBsAgS VLPs	791:827	WT and hyper-glycosylated HBsAgS VLPs	791:827	Comparative mass spectrometry-based N-glycan profiling, gel electrophoresis, and immunoassays demonstrated that WT and hyper-glycosylated HBsAgS VLPs contain the same type and distribution of N-glycan structures, but the latter shows a higher glycan abundance per protein mass.
32284273	3	96	theme	HBsAgS	514:519	arg1	glycoengineering					479:494	glycoengineering	479:494	glycoengineering of N-glycosylated HBsAgS to generate hyper-glycosylated VLPs	479:555	Herein, we report that glycoengineering of N-glycosylated HBsAgS to generate hyper-glycosylated VLPs display an enhanced immunogenicity relative to the wild type (WT) HBsAgS VLPs when expressed in FreeStyle HEK 293F cells.
32284273	8	97	theme	HBsAgS	1723:1728	arg1	platforms					1742:1750	hepatitis B and improved HBsAgS VLP carrier platforms	1698:1750	hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering	1698:1773	The ability of hyper-glycosylated VLPs to promote potent anti-HBsAgS immune responses compared to VLPs with a native N-glycan level as well as non-glycosylated, yeast-derived HBsAgS VLPs opens exciting avenues for generating more efficacious VLP-based vaccines against hepatitis B and improved HBsAgS VLP carrier platforms using glycoengineering.
32469526	4	0	theme	composite	621:629	arg1	materials					631:639	composite materials	621:639	composite materials based on biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets for the first time	621:745	Herein, we report composite materials based on biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets for the first time.
32469526	4	1	theme	MoS2	711:714	arg1	nanosheets					717:726	biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets	650:726	biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets for the first time	650:745	Herein, we report composite materials based on biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets for the first time.
32469526	8	2	theme	energy	1618:1623	arg1	density					1625:1631	a high discharge energy density	1601:1631	a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3)	1601:1706	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	3	3	theme	biodegradable	567:579	arg1	materials					592:600	new biodegradable dielectric materials	563:600	new biodegradable dielectric materials	563:600	However, the problems of environmental pollution caused by polymer-based dielectric materials have been extensively studied in recent years, which need to be solved urgently, leading to the search for new biodegradable dielectric materials.
32469526	8	4	theme	neat	1682:1685	arg1	cm-3					1702:1705	2.17 J cm-3	1695:1705	2.17 J cm-3	1695:1705	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	8	4	theme	neat	1682:1685	arg1	chitin					1687:1692	the neat chitin	1678:1692	the neat chitin (2.17 J cm-3)	1678:1706	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	2	5	theme	candidates	350:359	arg1	candidates					350:359	the most promising candidates	331:359	the most promising candidates	331:359	Among various dielectric materials, polymer nanocomposite is one of the most promising candidates.
32469526	2	5	theme	candidates	350:359	arg1	one					324:326	one	324:326	one	324:326	Among various dielectric materials, polymer nanocomposite is one of the most promising candidates.
32469526	6	6	theme	breakdown	1180:1188	arg1	barrier					1190:1196	a charge breakdown barrier	1171:1196	a charge breakdown barrier	1171:1196	The two-dimensional MoS2 nanosheets possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin; meanwhile, it can serve as a charge breakdown barrier to hinder the propagation of electrical tree branches.
32469526	6	6	theme	breakdown	1180:1188	arg1	meanwhile					1144:1152	meanwhile	1144:1152	meanwhile	1144:1152	The two-dimensional MoS2 nanosheets possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin; meanwhile, it can serve as a charge breakdown barrier to hinder the propagation of electrical tree branches.
32469526	2	7	theme	most	335:338	arg1	candidates					350:359	the most promising candidates	331:359	the most promising candidates	331:359	Among various dielectric materials, polymer nanocomposite is one of the most promising candidates.
32469526	8	8	theme	J	1700:1700	arg1	cm-3					1702:1705	2.17 J cm-3	1695:1705	2.17 J cm-3	1695:1705	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	8	8	theme	J	1700:1700	arg1	chitin					1687:1692	the neat chitin	1678:1692	the neat chitin (2.17 J cm-3)	1678:1706	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	6	9	theme	MoS2	1004:1007	arg1	nanosheets					1009:1018	The two-dimensional MoS2 nanosheets	984:1018	The two-dimensional MoS2 nanosheets	984:1018	The two-dimensional MoS2 nanosheets possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin; meanwhile, it can serve as a charge breakdown barrier to hinder the propagation of electrical tree branches.
32469526	4	10	theme	disulfide	700:708	arg1	nanosheets					717:726	biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets	650:726	biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets for the first time	650:745	Herein, we report composite materials based on biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets for the first time.
32469526	2	11	theme	various	269:275	arg1	materials					288:296	various dielectric materials	269:296	various dielectric materials	269:296	Among various dielectric materials, polymer nanocomposite is one of the most promising candidates.
32469526	1	12	theme	storage	254:260	arg1	field					238:242	the field	234:242	the field of energy storage	234:260	High-performance dielectric nanomaterials have received increasing attention due to their important applications in the field of energy storage.
32469526	2	13	theme	polymer	299:305	arg1	nanocomposite					307:319	polymer nanocomposite	299:319	polymer nanocomposite	299:319	Among various dielectric materials, polymer nanocomposite is one of the most promising candidates.
32469526	5	14	theme	green	969:973	arg1	process					975:981	a simple green process	960:981	a simple green process	960:981	The MoS2 nanosheets were first fabricated by glycerol/urea system and then KOH/urea aqueous solution was used to directly dissolve chitin at low temperature together with the dispersion of the MoS2 nanosheets in a simple green process.
32469526	6	15	theme	specific	1068:1075	arg1	area					1085:1088	a large specific surface area	1060:1088	a large specific surface area	1060:1088	The two-dimensional MoS2 nanosheets possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin; meanwhile, it can serve as a charge breakdown barrier to hinder the propagation of electrical tree branches.
32469526	8	16	theme	film	1506:1509	arg1	efficiencies					1476:1487	the charge and discharge efficiencies	1451:1487	efficiencies	1476:1487	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	8	16	theme	film	1506:1509	arg1	%					1528:1528	more than 80%	1516:1528	more than 80%	1516:1528	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	7	17	theme	dielectric	1387:1396	arg1	loss					1398:1401	the dielectric loss	1383:1401	the dielectric loss	1383:1401	The results also show that the dielectric constant and breakdown strength of the chitin/MoS2 nanocomposites were increased, while the dielectric loss remained low.
32469526	0	18	theme	Discharge	77:85	arg1	Density					94:100	Significantly Enhanced Discharge Energy Density	54:100	Significantly Enhanced Discharge Energy Density	54:100	Chitin/MoS2 Nanosheet Dielectric Composite Films with Significantly Enhanced Discharge Energy Density and Efficiency.
32469526	1	19	theme	due	195:197	arg1	attention					185:193	increasing attention	174:193	increasing attention due to their important applications in the field of energy storage	174:260	High-performance dielectric nanomaterials have received increasing attention due to their important applications in the field of energy storage.
32469526	5	20	theme	nanosheets	946:955	arg1	dispersion					923:932	the dispersion	919:932	the dispersion of the MoS2 nanosheets in a simple green process	919:981	The MoS2 nanosheets were first fabricated by glycerol/urea system and then KOH/urea aqueous solution was used to directly dissolve chitin at low temperature together with the dispersion of the MoS2 nanosheets in a simple green process.
32469526	3	21	theme	pollution	401:409	arg1	problems					375:382	the problems	371:382	the problems of environmental pollution caused by polymer-based dielectric materials	371:454	However, the problems of environmental pollution caused by polymer-based dielectric materials have been extensively studied in recent years, which need to be solved urgently, leading to the search for new biodegradable dielectric materials.
32469526	7	22	dep	constant	1295:1302	arg1	the					1280:1282	the	1280:1282	the	1280:1282	The results also show that the dielectric constant and breakdown strength of the chitin/MoS2 nanocomposites were increased, while the dielectric loss remained low.
32469526	6	23	theme	polarization	1033:1044	arg1	strength					1046:1053	high polarization strength	1028:1053	high polarization strength	1028:1053	The two-dimensional MoS2 nanosheets possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin; meanwhile, it can serve as a charge breakdown barrier to hinder the propagation of electrical tree branches.
32469526	9	24	theme	nanocomposite	1726:1738	arg1	films					1740:1744	the nanocomposite films	1722:1744	the nanocomposite films	1722:1744	Furthermore, the nanocomposite films exhibited good thermal stability.
32469526	3	25	theme	dielectric	435:444	arg1	materials					446:454	polymer-based dielectric materials	421:454	polymer-based dielectric materials	421:454	However, the problems of environmental pollution caused by polymer-based dielectric materials have been extensively studied in recent years, which need to be solved urgently, leading to the search for new biodegradable dielectric materials.
32469526	1	26	theme	increasing	174:183	arg1	attention					185:193	increasing attention	174:193	increasing attention due to their important applications in the field of energy storage	174:260	High-performance dielectric nanomaterials have received increasing attention due to their important applications in the field of energy storage.
32469526	0	27	theme	Energy	87:92	arg1	Density					94:100	Significantly Enhanced Discharge Energy Density	54:100	Significantly Enhanced Discharge Energy Density	54:100	Chitin/MoS2 Nanosheet Dielectric Composite Films with Significantly Enhanced Discharge Energy Density and Efficiency.
32469526	0	28	theme	Chitin/MoS2	0:10	arg1	Films					43:47	Chitin/MoS2 Nanosheet Dielectric Composite Films	0:47	Chitin/MoS2 Nanosheet Dielectric Composite Films with Significantly Enhanced Discharge Energy Density and Efficiency.	0:116	Chitin/MoS2 Nanosheet Dielectric Composite Films with Significantly Enhanced Discharge Energy Density and Efficiency.
32469526	6	29	dep	possess	1020:1026	arg1	enhance					1094:1100	enhance	1094:1100	possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin	1020:1141	The two-dimensional MoS2 nanosheets possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin; meanwhile, it can serve as a charge breakdown barrier to hinder the propagation of electrical tree branches.
32469526	6	30	theme	interfacial	1106:1116	arg1	polarization					1118:1129	the interfacial polarization	1102:1129	the interfacial polarization with chitin	1102:1141	The two-dimensional MoS2 nanosheets possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin; meanwhile, it can serve as a charge breakdown barrier to hinder the propagation of electrical tree branches.
32469526	9	31	theme	thermal	1761:1767	arg1	stability					1769:1777	good thermal stability	1756:1777	good thermal stability	1756:1777	Furthermore, the nanocomposite films exhibited good thermal stability.
32469526	6	32	theme	tree	1238:1241	arg1	branches					1243:1250	electrical tree branches	1227:1250	electrical tree branches	1227:1250	The two-dimensional MoS2 nanosheets possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin; meanwhile, it can serve as a charge breakdown barrier to hinder the propagation of electrical tree branches.
32469526	8	33	theme	MoS2	1426:1429	arg1	content					1431:1437	the MoS2 content	1422:1437	the MoS2 content	1422:1437	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	8	33	theme	MoS2	1426:1429	arg1	%					1448:1448	5 wt %	1443:1448	5 wt %	1443:1448	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	0	34	theme	Dielectric	22:31	arg1	Films					43:47	Chitin/MoS2 Nanosheet Dielectric Composite Films	0:47	Chitin/MoS2 Nanosheet Dielectric Composite Films with Significantly Enhanced Discharge Energy Density and Efficiency.	0:116	Chitin/MoS2 Nanosheet Dielectric Composite Films with Significantly Enhanced Discharge Energy Density and Efficiency.
32469526	6	35	with	polarization	1118:1129	arg1	chitin					1136:1141	chitin	1136:1141	chitin	1136:1141	The two-dimensional MoS2 nanosheets possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin; meanwhile, it can serve as a charge breakdown barrier to hinder the propagation of electrical tree branches.
32469526	1	36	theme	dielectric	135:144	arg1	nanomaterials					146:158	High-performance dielectric nanomaterials	118:158	High-performance dielectric nanomaterials	118:158	High-performance dielectric nanomaterials have received increasing attention due to their important applications in the field of energy storage.
32469526	5	37	theme	aqueous	832:838	arg1	solution					840:847	KOH/urea aqueous solution	823:847	KOH/urea aqueous solution	823:847	The MoS2 nanosheets were first fabricated by glycerol/urea system and then KOH/urea aqueous solution was used to directly dissolve chitin at low temperature together with the dispersion of the MoS2 nanosheets in a simple green process.
32469526	7	38	theme	chitin/MoS2	1334:1344	arg1	nanocomposites					1346:1359	the chitin/MoS2 nanocomposites	1330:1359	the chitin/MoS2 nanocomposites	1330:1359	The results also show that the dielectric constant and breakdown strength of the chitin/MoS2 nanocomposites were increased, while the dielectric loss remained low.
32469526	8	39	theme	MV	1575:1576	arg1	m-1					1578:1580	350 MV m-1	1571:1580	350 MV m-1	1571:1580	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	10	40	theme	chitin-based	1797:1808	arg1	films					1824:1828	these chitin-based nanocomposite films	1791:1828	these chitin-based nanocomposite films	1791:1828	Therefore, these chitin-based nanocomposite films are promising as high-performance biomass-based dielectric capacitors.
32469526	6	41	contain	possess	1020:1026	arg1	nanosheets					1009:1018	The two-dimensional MoS2 nanosheets	984:1018	The two-dimensional MoS2 nanosheets	984:1018	The two-dimensional MoS2 nanosheets possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin; meanwhile, it can serve as a charge breakdown barrier to hinder the propagation of electrical tree branches.
32469526	6	41	contain	possess	1020:1026	arg2	strength					1046:1053	high polarization strength	1028:1053	high polarization strength	1028:1053	The two-dimensional MoS2 nanosheets possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin; meanwhile, it can serve as a charge breakdown barrier to hinder the propagation of electrical tree branches.
32469526	6	41	contain	possess	1020:1026	arg2	area					1085:1088	a large specific surface area	1060:1088	a large specific surface area	1060:1088	The two-dimensional MoS2 nanosheets possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin; meanwhile, it can serve as a charge breakdown barrier to hinder the propagation of electrical tree branches.
32469526	10	42	theme	high-performance	1847:1862	arg1	capacitors					1889:1898	high-performance biomass-based dielectric capacitors	1847:1898	high-performance biomass-based dielectric capacitors	1847:1898	Therefore, these chitin-based nanocomposite films are promising as high-performance biomass-based dielectric capacitors.
32469526	8	43	theme	4.91	1636:1639	arg1	cm-3					1643:1646	4.91 J cm-3	1636:1646	4.91 J cm-3	1636:1646	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	5	44	used	used	853:856	arg2	solution					840:847	KOH/urea aqueous solution	823:847	KOH/urea aqueous solution	823:847	The MoS2 nanosheets were first fabricated by glycerol/urea system and then KOH/urea aqueous solution was used to directly dissolve chitin at low temperature together with the dispersion of the MoS2 nanosheets in a simple green process.
32469526	7	45	theme	breakdown	1308:1316	arg1	strength					1318:1325	breakdown strength	1308:1325	breakdown strength	1308:1325	The results also show that the dielectric constant and breakdown strength of the chitin/MoS2 nanocomposites were increased, while the dielectric loss remained low.
32469526	8	46	theme	charge	1455:1460	arg1	efficiencies					1476:1487	the charge and discharge efficiencies	1451:1487	efficiencies	1476:1487	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	8	46	theme	charge	1455:1460	arg1	%					1528:1528	more than 80%	1516:1528	more than 80%	1516:1528	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	8	47	theme	cm-3	1643:1646	arg1	density					1625:1631	a high discharge energy density	1601:1631	a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3)	1601:1706	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	0	48	with	Films	43:47	arg1	Density					94:100	Significantly Enhanced Discharge Energy Density	54:100	Significantly Enhanced Discharge Energy Density	54:100	Chitin/MoS2 Nanosheet Dielectric Composite Films with Significantly Enhanced Discharge Energy Density and Efficiency.
32469526	0	48	with	Films	43:47	arg1	Efficiency					106:115	Efficiency	106:115	Efficiency	106:115	Chitin/MoS2 Nanosheet Dielectric Composite Films with Significantly Enhanced Discharge Energy Density and Efficiency.
32469526	8	49	theme	discharge	1608:1616	arg1	density					1625:1631	a high discharge energy density	1601:1631	a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3)	1601:1706	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	10	50	theme	dielectric	1878:1887	arg1	capacitors					1889:1898	high-performance biomass-based dielectric capacitors	1847:1898	high-performance biomass-based dielectric capacitors	1847:1898	Therefore, these chitin-based nanocomposite films are promising as high-performance biomass-based dielectric capacitors.
32469526	8	51	theme	wt	1445:1446	arg1	content					1431:1437	the MoS2 content	1422:1437	the MoS2 content	1422:1437	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	8	51	theme	wt	1445:1446	arg1	%					1448:1448	5 wt %	1443:1448	5 wt %	1443:1448	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	3	52	theme	dielectric	581:590	arg1	materials					592:600	new biodegradable dielectric materials	563:600	new biodegradable dielectric materials	563:600	However, the problems of environmental pollution caused by polymer-based dielectric materials have been extensively studied in recent years, which need to be solved urgently, leading to the search for new biodegradable dielectric materials.
32469526	3	53	theme	new	563:565	arg1	materials					592:600	new biodegradable dielectric materials	563:600	new biodegradable dielectric materials	563:600	However, the problems of environmental pollution caused by polymer-based dielectric materials have been extensively studied in recent years, which need to be solved urgently, leading to the search for new biodegradable dielectric materials.
32469526	1	54	theme	important	208:216	arg1	applications					218:229	their important applications	202:229	their important applications in the field of energy storage	202:260	High-performance dielectric nanomaterials have received increasing attention due to their important applications in the field of energy storage.
32469526	6	55	theme	high	1028:1031	arg1	strength					1046:1053	high polarization strength	1028:1053	high polarization strength	1028:1053	The two-dimensional MoS2 nanosheets possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin; meanwhile, it can serve as a charge breakdown barrier to hinder the propagation of electrical tree branches.
32469526	2	56	theme	promising	340:348	arg1	candidates					350:359	the most promising candidates	331:359	the most promising candidates	331:359	Among various dielectric materials, polymer nanocomposite is one of the most promising candidates.
32469526	8	57	theme	discharge	1466:1474	arg1	efficiencies					1476:1487	the charge and discharge efficiencies	1451:1487	efficiencies	1476:1487	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	8	57	theme	discharge	1466:1474	arg1	%					1528:1528	more than 80%	1516:1528	more than 80%	1516:1528	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	6	58	theme	charge	1173:1178	arg1	barrier					1190:1196	a charge breakdown barrier	1171:1196	a charge breakdown barrier	1171:1196	The two-dimensional MoS2 nanosheets possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin; meanwhile, it can serve as a charge breakdown barrier to hinder the propagation of electrical tree branches.
32469526	6	58	theme	charge	1173:1178	arg1	meanwhile					1144:1152	meanwhile	1144:1152	meanwhile	1144:1152	The two-dimensional MoS2 nanosheets possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin; meanwhile, it can serve as a charge breakdown barrier to hinder the propagation of electrical tree branches.
32469526	4	59	theme	chitin	678:683	arg1	nanosheets					717:726	biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets	650:726	biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets for the first time	650:745	Herein, we report composite materials based on biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets for the first time.
32469526	5	60	theme	MoS2	752:755	arg1	nanosheets					757:766	The MoS2 nanosheets	748:766	The MoS2 nanosheets	748:766	The MoS2 nanosheets were first fabricated by glycerol/urea system and then KOH/urea aqueous solution was used to directly dissolve chitin at low temperature together with the dispersion of the MoS2 nanosheets in a simple green process.
32469526	2	61	theme	dielectric	277:286	arg1	materials					288:296	various dielectric materials	269:296	various dielectric materials	269:296	Among various dielectric materials, polymer nanocomposite is one of the most promising candidates.
32469526	5	62	theme	low	889:891	arg1	temperature					893:903	low temperature	889:903	low temperature	889:903	The MoS2 nanosheets were first fabricated by glycerol/urea system and then KOH/urea aqueous solution was used to directly dissolve chitin at low temperature together with the dispersion of the MoS2 nanosheets in a simple green process.
32469526	6	63	theme	two-dimensional	988:1002	arg1	nanosheets					1009:1018	The two-dimensional MoS2 nanosheets	984:1018	The two-dimensional MoS2 nanosheets	984:1018	The two-dimensional MoS2 nanosheets possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin; meanwhile, it can serve as a charge breakdown barrier to hinder the propagation of electrical tree branches.
32469526	4	64	theme	molybdenum	689:698	arg1	nanosheets					717:726	biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets	650:726	biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets for the first time	650:745	Herein, we report composite materials based on biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets for the first time.
32469526	8	65	theme	composite	1496:1504	arg1	film					1506:1509	the composite film	1492:1509	the composite film	1492:1509	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	5	66	theme	simple	962:967	arg1	process					975:981	a simple green process	960:981	a simple green process	960:981	The MoS2 nanosheets were first fabricated by glycerol/urea system and then KOH/urea aqueous solution was used to directly dissolve chitin at low temperature together with the dispersion of the MoS2 nanosheets in a simple green process.
32469526	6	67	theme	surface	1077:1083	arg1	area					1085:1088	a large specific surface area	1060:1088	a large specific surface area	1060:1088	The two-dimensional MoS2 nanosheets possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin; meanwhile, it can serve as a charge breakdown barrier to hinder the propagation of electrical tree branches.
32469526	6	68	theme	branches	1243:1250	arg1	propagation					1212:1222	the propagation	1208:1222	the propagation of electrical tree branches	1208:1250	The two-dimensional MoS2 nanosheets possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin; meanwhile, it can serve as a charge breakdown barrier to hinder the propagation of electrical tree branches.
32469526	5	69	theme	glycerol/urea	793:805	arg1	system					807:812	glycerol/urea system	793:812	glycerol/urea system	793:812	The MoS2 nanosheets were first fabricated by glycerol/urea system and then KOH/urea aqueous solution was used to directly dissolve chitin at low temperature together with the dispersion of the MoS2 nanosheets in a simple green process.
32469526	5	70	from	dispersion	923:932	arg1	process					975:981	a simple green process	960:981	a simple green process	960:981	The MoS2 nanosheets were first fabricated by glycerol/urea system and then KOH/urea aqueous solution was used to directly dissolve chitin at low temperature together with the dispersion of the MoS2 nanosheets in a simple green process.
32469526	6	71	theme	large	1062:1066	arg1	area					1085:1088	a large specific surface area	1060:1088	a large specific surface area	1060:1088	The two-dimensional MoS2 nanosheets possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin; meanwhile, it can serve as a charge breakdown barrier to hinder the propagation of electrical tree branches.
32469526	6	72	theme	electrical	1227:1236	arg1	branches					1243:1250	electrical tree branches	1227:1250	electrical tree branches	1227:1250	The two-dimensional MoS2 nanosheets possess high polarization strength, and a large specific surface area can enhance the interfacial polarization with chitin; meanwhile, it can serve as a charge breakdown barrier to hinder the propagation of electrical tree branches.
32469526	1	73	from	applications	218:229	arg1	field					238:242	the field	234:242	the field of energy storage	234:260	High-performance dielectric nanomaterials have received increasing attention due to their important applications in the field of energy storage.
32469526	5	74	theme	MoS2	941:944	arg1	nanosheets					946:955	the MoS2 nanosheets	937:955	the MoS2 nanosheets in a simple green process	937:981	The MoS2 nanosheets were first fabricated by glycerol/urea system and then KOH/urea aqueous solution was used to directly dissolve chitin at low temperature together with the dispersion of the MoS2 nanosheets in a simple green process.
32469526	4	75	theme	first	736:740	arg1	time					742:745	the first time	732:745	the first time	732:745	Herein, we report composite materials based on biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets for the first time.
32469526	5	76	from	nanosheets	946:955	arg1	process					975:981	a simple green process	960:981	a simple green process	960:981	The MoS2 nanosheets were first fabricated by glycerol/urea system and then KOH/urea aqueous solution was used to directly dissolve chitin at low temperature together with the dispersion of the MoS2 nanosheets in a simple green process.
32469526	3	77	theme	environmental	387:399	arg1	pollution					401:409	environmental pollution	387:409	environmental pollution caused by polymer-based dielectric materials	387:454	However, the problems of environmental pollution caused by polymer-based dielectric materials have been extensively studied in recent years, which need to be solved urgently, leading to the search for new biodegradable dielectric materials.
32469526	8	78	theme	breakdown	1539:1547	arg1	strength					1549:1556	the breakdown strength	1535:1556	the breakdown strength	1535:1556	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	0	79	theme	Nanosheet	12:20	arg1	Films					43:47	Chitin/MoS2 Nanosheet Dielectric Composite Films	0:47	Chitin/MoS2 Nanosheet Dielectric Composite Films with Significantly Enhanced Discharge Energy Density and Efficiency.	0:116	Chitin/MoS2 Nanosheet Dielectric Composite Films with Significantly Enhanced Discharge Energy Density and Efficiency.
32469526	9	80	theme	good	1756:1759	arg1	stability					1769:1777	good thermal stability	1756:1777	good thermal stability	1756:1777	Furthermore, the nanocomposite films exhibited good thermal stability.
32469526	3	81	theme	recent	489:494	arg1	years					496:500	recent years	489:500	recent years	489:500	However, the problems of environmental pollution caused by polymer-based dielectric materials have been extensively studied in recent years, which need to be solved urgently, leading to the search for new biodegradable dielectric materials.
32469526	0	82	theme	Composite	33:41	arg1	Films					43:47	Chitin/MoS2 Nanosheet Dielectric Composite Films	0:47	Chitin/MoS2 Nanosheet Dielectric Composite Films with Significantly Enhanced Discharge Energy Density and Efficiency.	0:116	Chitin/MoS2 Nanosheet Dielectric Composite Films with Significantly Enhanced Discharge Energy Density and Efficiency.
32469526	1	83	theme	High-performance	118:133	arg1	nanomaterials					146:158	High-performance dielectric nanomaterials	118:158	High-performance dielectric nanomaterials	118:158	High-performance dielectric nanomaterials have received increasing attention due to their important applications in the field of energy storage.
32469526	7	84	theme	nanocomposites	1346:1359	arg1	constant					1295:1302	dielectric constant	1284:1302	dielectric constant	1284:1302	The results also show that the dielectric constant and breakdown strength of the chitin/MoS2 nanocomposites were increased, while the dielectric loss remained low.
32469526	7	84	theme	nanocomposites	1346:1359	arg1	strength					1318:1325	breakdown strength	1308:1325	breakdown strength	1308:1325	The results also show that the dielectric constant and breakdown strength of the chitin/MoS2 nanocomposites were increased, while the dielectric loss remained low.
32469526	3	85	theme	polymer-based	421:433	arg1	materials					446:454	polymer-based dielectric materials	421:454	polymer-based dielectric materials	421:454	However, the problems of environmental pollution caused by polymer-based dielectric materials have been extensively studied in recent years, which need to be solved urgently, leading to the search for new biodegradable dielectric materials.
32469526	5	86	theme	KOH/urea	823:830	arg1	solution					840:847	KOH/urea aqueous solution	823:847	KOH/urea aqueous solution	823:847	The MoS2 nanosheets were first fabricated by glycerol/urea system and then KOH/urea aqueous solution was used to directly dissolve chitin at low temperature together with the dispersion of the MoS2 nanosheets in a simple green process.
32469526	1	87	theme	energy	247:252	arg1	storage					254:260	energy storage	247:260	energy storage	247:260	High-performance dielectric nanomaterials have received increasing attention due to their important applications in the field of energy storage.
32469526	10	88	theme	nanocomposite	1810:1822	arg1	films					1824:1828	these chitin-based nanocomposite films	1791:1828	these chitin-based nanocomposite films	1791:1828	Therefore, these chitin-based nanocomposite films are promising as high-performance biomass-based dielectric capacitors.
32469526	8	89	theme	high	1603:1606	arg1	density					1625:1631	a high discharge energy density	1601:1631	a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3)	1601:1706	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	0	90	theme	Enhanced	68:75	arg1	Density					94:100	Significantly Enhanced Discharge Energy Density	54:100	Significantly Enhanced Discharge Energy Density	54:100	Chitin/MoS2 Nanosheet Dielectric Composite Films with Significantly Enhanced Discharge Energy Density and Efficiency.
32469526	10	91	theme	biomass-based	1864:1876	arg1	capacitors					1889:1898	high-performance biomass-based dielectric capacitors	1847:1898	high-performance biomass-based dielectric capacitors	1847:1898	Therefore, these chitin-based nanocomposite films are promising as high-performance biomass-based dielectric capacitors.
32469526	8	92	theme	J	1641:1641	arg1	cm-3					1643:1646	4.91 J cm-3	1636:1646	4.91 J cm-3	1636:1646	When the MoS2 content was 5 wt %, the charge and discharge efficiencies of the composite film were more than 80%, and the breakdown strength also reached 350 MV m-1, thus resulting in a high discharge energy density of 4.91 J cm-3, which was more than twice of the neat chitin (2.17 J cm-3).
32469526	4	93	theme	biodegradable	650:662	arg1	nanosheets					717:726	biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets	650:726	biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets for the first time	650:745	Herein, we report composite materials based on biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets for the first time.
32469526	5	94	from	process	975:981	arg1	dispersion					923:932	the dispersion	919:932	the dispersion of the MoS2 nanosheets in a simple green process	919:981	The MoS2 nanosheets were first fabricated by glycerol/urea system and then KOH/urea aqueous solution was used to directly dissolve chitin at low temperature together with the dispersion of the MoS2 nanosheets in a simple green process.
32469526	7	95	theme	dielectric	1284:1293	arg1	constant					1295:1302	dielectric constant	1284:1302	dielectric constant	1284:1302	The results also show that the dielectric constant and breakdown strength of the chitin/MoS2 nanocomposites were increased, while the dielectric loss remained low.
32469526	4	96	theme	renewable	668:676	arg1	nanosheets					717:726	biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets	650:726	biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets for the first time	650:745	Herein, we report composite materials based on biodegradable and renewable chitin and molybdenum disulfide (MoS2) nanosheets for the first time.
33735774	0	0	theme	enzymatic-fermentative	97:118	arg1	method					120:125	enzymatic-fermentative method	97:125	enzymatic-fermentative method	97:125	Production of a synbiotic composed of galacto-oligosaccharides and Saccharomyces boulardii using enzymatic-fermentative method.
33735774	2	1	used	used	513:516	arg2	design					484:489	A central composite rotatable design	454:489	A central composite rotatable design with center point	454:507	A central composite rotatable design with center point was used to evaluate the influence of temperature and enzyme concentration in the GOS and S. boulardii production.
33735774	1	2	theme	sustainable	181:191	arg1	processes					207:215	sustainable technological processes	181:215	sustainable technological processes	181:215	Motivated by the search for healthy alimentation and sustainable technological processes, this study aimed to produce a synbiotic composed of the prebiotic galacto-oligosaccharides (GOS) and the probiotic yeast Saccharomyces boulardii, simultaneously, using cheese whey permeate as substrate by enzymatic-fermentative method.
33735774	1	3	theme	prebiotic	274:282	arg1	galacto-oligosaccharides					284:307	the prebiotic galacto-oligosaccharides	270:307	the prebiotic galacto-oligosaccharides (GOS)	270:313	Motivated by the search for healthy alimentation and sustainable technological processes, this study aimed to produce a synbiotic composed of the prebiotic galacto-oligosaccharides (GOS) and the probiotic yeast Saccharomyces boulardii, simultaneously, using cheese whey permeate as substrate by enzymatic-fermentative method.
33735774	1	3	theme	prebiotic	274:282	arg1	GOS					310:312	GOS	310:312	GOS	310:312	Motivated by the search for healthy alimentation and sustainable technological processes, this study aimed to produce a synbiotic composed of the prebiotic galacto-oligosaccharides (GOS) and the probiotic yeast Saccharomyces boulardii, simultaneously, using cheese whey permeate as substrate by enzymatic-fermentative method.
33735774	5	4	theme	dairy	1153:1157	arg1	lines					1170:1174	dairy production lines	1153:1174	dairy production lines as functional food	1153:1193	The simultaneous enzymatic-fermentative method showed promising results considering industrial application and can be easily incorporated into dairy production lines as functional food.
33735774	5	5	theme	production	1159:1168	arg1	lines					1170:1174	dairy production lines	1153:1174	dairy production lines as functional food	1153:1193	The simultaneous enzymatic-fermentative method showed promising results considering industrial application and can be easily incorporated into dairy production lines as functional food.
33735774	1	6	theme	technological	193:205	arg1	processes					207:215	sustainable technological processes	181:215	sustainable technological processes	181:215	Motivated by the search for healthy alimentation and sustainable technological processes, this study aimed to produce a synbiotic composed of the prebiotic galacto-oligosaccharides (GOS) and the probiotic yeast Saccharomyces boulardii, simultaneously, using cheese whey permeate as substrate by enzymatic-fermentative method.
33735774	3	7	theme	enzyme	684:689	arg1	concentration					691:703	enzyme concentration	684:703	enzyme concentration	684:703	The best condition to obtain the prebiotic was at 32 °C and enzyme concentration of 0.175% (w/w), providing 56.84 g L-1 of GOS concentration and Ln(3.59) 107 viable cells mL-1 of S. boulardii production.
33735774	2	8	dep	GOS	591:593	arg1	the					587:589	the	587:589	the	587:589	A central composite rotatable design with center point was used to evaluate the influence of temperature and enzyme concentration in the GOS and S. boulardii production.
33735774	1	9	theme	cheese	386:391	arg1	whey					393:396	cheese whey	386:396	cheese whey	386:396	Motivated by the search for healthy alimentation and sustainable technological processes, this study aimed to produce a synbiotic composed of the prebiotic galacto-oligosaccharides (GOS) and the probiotic yeast Saccharomyces boulardii, simultaneously, using cheese whey permeate as substrate by enzymatic-fermentative method.
33735774	5	10	theme	simultaneous	1014:1025	arg1	method					1050:1055	The simultaneous enzymatic-fermentative method	1010:1055	The simultaneous enzymatic-fermentative method	1010:1055	The simultaneous enzymatic-fermentative method showed promising results considering industrial application and can be easily incorporated into dairy production lines as functional food.
33735774	3	11	theme	production	816:825	arg1	mL-1					795:798	Ln(3.59) 107 viable cells mL-1	769:798	Ln(3.59) 107 viable cells mL-1 of S. boulardii production	769:825	The best condition to obtain the prebiotic was at 32 °C and enzyme concentration of 0.175% (w/w), providing 56.84 g L-1 of GOS concentration and Ln(3.59) 107 viable cells mL-1 of S. boulardii production.
33735774	3	11	theme	production	816:825	arg1	56.84 g L-1					732:742	56.84 g L-1	732:742	56.84 g L-1 of GOS concentration	732:763	The best condition to obtain the prebiotic was at 32 °C and enzyme concentration of 0.175% (w/w), providing 56.84 g L-1 of GOS concentration and Ln(3.59) 107 viable cells mL-1 of S. boulardii production.
33735774	2	12	theme	concentration	570:582	arg1	influence					534:542	the influence	530:542	the influence of temperature and enzyme concentration in the GOS and S. boulardii production	530:621	A central composite rotatable design with center point was used to evaluate the influence of temperature and enzyme concentration in the GOS and S. boulardii production.
33735774	3	13	theme	GOS	747:749	arg1	concentration					751:763	GOS concentration	747:763	GOS concentration	747:763	The best condition to obtain the prebiotic was at 32 °C and enzyme concentration of 0.175% (w/w), providing 56.84 g L-1 of GOS concentration and Ln(3.59) 107 viable cells mL-1 of S. boulardii production.
33735774	4	14	theme	simultaneous	872:883	arg1	production					885:894	the simultaneous production	868:894	the simultaneous production of GOS	868:901	However, the condition that would favor the simultaneous production of GOS and S. boulardii studied through desirability function is 29.5 °C and 0.14% (w/w) of enzyme concentration.
33735774	5	15	dep	showed	1057:1062	arg1	incorporated					1135:1146	incorporated	1135:1146	can be easily incorporated into dairy production lines as functional food	1121:1193	The simultaneous enzymatic-fermentative method showed promising results considering industrial application and can be easily incorporated into dairy production lines as functional food.
33735774	5	15	dep	showed	1057:1062	arg1	considering					1082:1092	considering	1082:1092	considering industrial application	1082:1115	The simultaneous enzymatic-fermentative method showed promising results considering industrial application and can be easily incorporated into dairy production lines as functional food.
33735774	2	16	theme	enzyme	563:568	arg1	concentration					570:582	enzyme concentration	563:582	enzyme concentration	563:582	A central composite rotatable design with center point was used to evaluate the influence of temperature and enzyme concentration in the GOS and S. boulardii production.
33735774	3	17	theme	concentration	751:763	arg1	mL-1					795:798	Ln(3.59) 107 viable cells mL-1	769:798	Ln(3.59) 107 viable cells mL-1 of S. boulardii production	769:825	The best condition to obtain the prebiotic was at 32 °C and enzyme concentration of 0.175% (w/w), providing 56.84 g L-1 of GOS concentration and Ln(3.59) 107 viable cells mL-1 of S. boulardii production.
33735774	3	17	theme	concentration	751:763	arg1	56.84 g L-1					732:742	56.84 g L-1	732:742	56.84 g L-1 of GOS concentration	732:763	The best condition to obtain the prebiotic was at 32 °C and enzyme concentration of 0.175% (w/w), providing 56.84 g L-1 of GOS concentration and Ln(3.59) 107 viable cells mL-1 of S. boulardii production.
33735774	1	18	dep	yeast	333:337	arg1	boulardii					353:361	Saccharomyces boulardii	339:361	the probiotic yeast Saccharomyces boulardii	319:361	Motivated by the search for healthy alimentation and sustainable technological processes, this study aimed to produce a synbiotic composed of the prebiotic galacto-oligosaccharides (GOS) and the probiotic yeast Saccharomyces boulardii, simultaneously, using cheese whey permeate as substrate by enzymatic-fermentative method.
33735774	2	19	dep	S.	599:600	arg1	boulardii					602:610	boulardii	602:610	boulardii	602:610	A central composite rotatable design with center point was used to evaluate the influence of temperature and enzyme concentration in the GOS and S. boulardii production.
33735774	3	20	theme	Ln	769:770	arg1	cells					789:793	Ln(3.59) 107 viable cells	769:793	Ln(3.59) 107 viable cells mL-1 of S. boulardii production	769:825	The best condition to obtain the prebiotic was at 32 °C and enzyme concentration of 0.175% (w/w), providing 56.84 g L-1 of GOS concentration and Ln(3.59) 107 viable cells mL-1 of S. boulardii production.
33735774	2	21	theme	center	496:501	arg1	point					503:507	center point	496:507	center point	496:507	A central composite rotatable design with center point was used to evaluate the influence of temperature and enzyme concentration in the GOS and S. boulardii production.
33735774	5	22	theme	industrial	1094:1103	arg1	application					1105:1115	industrial application	1094:1115	industrial application	1094:1115	The simultaneous enzymatic-fermentative method showed promising results considering industrial application and can be easily incorporated into dairy production lines as functional food.
33735774	0	23	theme	synbiotic	16:24	arg1	Production					0:9	Production	0:9	Production of a synbiotic	0:24	Production of a synbiotic composed of galacto-oligosaccharides and Saccharomyces boulardii using enzymatic-fermentative method.
33735774	2	24	theme	S.	599:600	arg1	production					612:621	S. boulardii production	599:621	S. boulardii production	599:621	A central composite rotatable design with center point was used to evaluate the influence of temperature and enzyme concentration in the GOS and S. boulardii production.
33735774	2	25	theme	rotatable	474:482	arg1	design					484:489	A central composite rotatable design	454:489	A central composite rotatable design with center point	454:507	A central composite rotatable design with center point was used to evaluate the influence of temperature and enzyme concentration in the GOS and S. boulardii production.
33735774	2	26	from	influence	534:542	arg1	production					612:621	S. boulardii production	599:621	S. boulardii production	599:621	A central composite rotatable design with center point was used to evaluate the influence of temperature and enzyme concentration in the GOS and S. boulardii production.
33735774	2	26	from	influence	534:542	arg1	GOS					591:593	GOS	591:593	GOS	591:593	A central composite rotatable design with center point was used to evaluate the influence of temperature and enzyme concentration in the GOS and S. boulardii production.
33735774	2	27	theme	composite	464:472	arg1	design					484:489	A central composite rotatable design	454:489	A central composite rotatable design with center point	454:507	A central composite rotatable design with center point was used to evaluate the influence of temperature and enzyme concentration in the GOS and S. boulardii production.
33735774	1	28	theme	probiotic	323:331	arg1	yeast					333:337	the probiotic yeast	319:337	the probiotic yeast Saccharomyces boulardii	319:361	Motivated by the search for healthy alimentation and sustainable technological processes, this study aimed to produce a synbiotic composed of the prebiotic galacto-oligosaccharides (GOS) and the probiotic yeast Saccharomyces boulardii, simultaneously, using cheese whey permeate as substrate by enzymatic-fermentative method.
33735774	4	29	theme	desirability	936:947	arg1	function					949:956	desirability function	936:956	desirability function	936:956	However, the condition that would favor the simultaneous production of GOS and S. boulardii studied through desirability function is 29.5 °C and 0.14% (w/w) of enzyme concentration.
33735774	1	30	theme	enzymatic-fermentative	423:444	arg1	method					446:451	enzymatic-fermentative method	423:451	enzymatic-fermentative method	423:451	Motivated by the search for healthy alimentation and sustainable technological processes, this study aimed to produce a synbiotic composed of the prebiotic galacto-oligosaccharides (GOS) and the probiotic yeast Saccharomyces boulardii, simultaneously, using cheese whey permeate as substrate by enzymatic-fermentative method.
33735774	2	31	theme	central	456:462	arg1	design					484:489	A central composite rotatable design	454:489	A central composite rotatable design with center point	454:507	A central composite rotatable design with center point was used to evaluate the influence of temperature and enzyme concentration in the GOS and S. boulardii production.
33735774	3	32	theme	viable	782:787	arg1	cells					789:793	Ln(3.59) 107 viable cells	769:793	Ln(3.59) 107 viable cells mL-1 of S. boulardii production	769:825	The best condition to obtain the prebiotic was at 32 °C and enzyme concentration of 0.175% (w/w), providing 56.84 g L-1 of GOS concentration and Ln(3.59) 107 viable cells mL-1 of S. boulardii production.
33735774	4	33	theme	concentration	995:1007	arg1	29.5 °C					961:967	29.5 °C	961:967	29.5 °C	961:967	However, the condition that would favor the simultaneous production of GOS and S. boulardii studied through desirability function is 29.5 °C and 0.14% (w/w) of enzyme concentration.
33735774	4	33	theme	concentration	995:1007	arg1	%					977:977	0.14%	973:977	0.14% (w/w) of enzyme concentration	973:1007	However, the condition that would favor the simultaneous production of GOS and S. boulardii studied through desirability function is 29.5 °C and 0.14% (w/w) of enzyme concentration.
33735774	4	33	theme	concentration	995:1007	arg1	concentration					995:1007	enzyme concentration	988:1007	enzyme concentration	988:1007	However, the condition that would favor the simultaneous production of GOS and S. boulardii studied through desirability function is 29.5 °C and 0.14% (w/w) of enzyme concentration.
33735774	4	33	theme	concentration	995:1007	arg1	condition					841:849	the condition	837:849	the condition that would favor the simultaneous production of GOS	837:901	However, the condition that would favor the simultaneous production of GOS and S. boulardii studied through desirability function is 29.5 °C and 0.14% (w/w) of enzyme concentration.
33735774	4	33	theme	concentration	995:1007	arg1	w/w					980:982	w/w	980:982	w/w	980:982	However, the condition that would favor the simultaneous production of GOS and S. boulardii studied through desirability function is 29.5 °C and 0.14% (w/w) of enzyme concentration.
33735774	3	34	theme	cells	789:793	arg1	mL-1					795:798	Ln(3.59) 107 viable cells mL-1	769:798	Ln(3.59) 107 viable cells mL-1 of S. boulardii production	769:825	The best condition to obtain the prebiotic was at 32 °C and enzyme concentration of 0.175% (w/w), providing 56.84 g L-1 of GOS concentration and Ln(3.59) 107 viable cells mL-1 of S. boulardii production.
33735774	4	35	theme	GOS	899:901	arg1	production					885:894	the simultaneous production	868:894	the simultaneous production of GOS	868:901	However, the condition that would favor the simultaneous production of GOS and S. boulardii studied through desirability function is 29.5 °C and 0.14% (w/w) of enzyme concentration.
33735774	3	36	theme	best	628:631	arg1	condition					633:641	The best condition	624:641	The best condition to obtain the prebiotic	624:665	The best condition to obtain the prebiotic was at 32 °C and enzyme concentration of 0.175% (w/w), providing 56.84 g L-1 of GOS concentration and Ln(3.59) 107 viable cells mL-1 of S. boulardii production.
33735774	2	37	with	design	484:489	arg1	point					503:507	center point	496:507	center point	496:507	A central composite rotatable design with center point was used to evaluate the influence of temperature and enzyme concentration in the GOS and S. boulardii production.
33735774	4	38	theme	enzyme	988:993	arg1	concentration					995:1007	enzyme concentration	988:1007	enzyme concentration	988:1007	However, the condition that would favor the simultaneous production of GOS and S. boulardii studied through desirability function is 29.5 °C and 0.14% (w/w) of enzyme concentration.
33735774	3	39	theme	%	713:713	arg1	32 °C					674:678	32 °C	674:678	32 °C	674:678	The best condition to obtain the prebiotic was at 32 °C and enzyme concentration of 0.175% (w/w), providing 56.84 g L-1 of GOS concentration and Ln(3.59) 107 viable cells mL-1 of S. boulardii production.
33735774	3	39	theme	%	713:713	arg1	concentration					691:703	enzyme concentration	684:703	enzyme concentration	684:703	The best condition to obtain the prebiotic was at 32 °C and enzyme concentration of 0.175% (w/w), providing 56.84 g L-1 of GOS concentration and Ln(3.59) 107 viable cells mL-1 of S. boulardii production.
33735774	5	40	theme	enzymatic-fermentative	1027:1048	arg1	method					1050:1055	The simultaneous enzymatic-fermentative method	1010:1055	The simultaneous enzymatic-fermentative method	1010:1055	The simultaneous enzymatic-fermentative method showed promising results considering industrial application and can be easily incorporated into dairy production lines as functional food.
33735774	3	41	theme	S.	803:804	arg1	production					816:825	S. boulardii production	803:825	S. boulardii production	803:825	The best condition to obtain the prebiotic was at 32 °C and enzyme concentration of 0.175% (w/w), providing 56.84 g L-1 of GOS concentration and Ln(3.59) 107 viable cells mL-1 of S. boulardii production.
33735774	5	42	theme	promising	1064:1072	arg1	results					1074:1080	promising results	1064:1080	promising results	1064:1080	The simultaneous enzymatic-fermentative method showed promising results considering industrial application and can be easily incorporated into dairy production lines as functional food.
33735774	1	43	theme	healthy	156:162	arg1	alimentation					164:175	healthy alimentation	156:175	healthy alimentation	156:175	Motivated by the search for healthy alimentation and sustainable technological processes, this study aimed to produce a synbiotic composed of the prebiotic galacto-oligosaccharides (GOS) and the probiotic yeast Saccharomyces boulardii, simultaneously, using cheese whey permeate as substrate by enzymatic-fermentative method.
33735774	3	44	theme	boulardii	806:814	arg1	production					816:825	S. boulardii production	803:825	S. boulardii production	803:825	The best condition to obtain the prebiotic was at 32 °C and enzyme concentration of 0.175% (w/w), providing 56.84 g L-1 of GOS concentration and Ln(3.59) 107 viable cells mL-1 of S. boulardii production.
33735774	2	45	theme	temperature	547:557	arg1	influence					534:542	the influence	530:542	the influence of temperature and enzyme concentration in the GOS and S. boulardii production	530:621	A central composite rotatable design with center point was used to evaluate the influence of temperature and enzyme concentration in the GOS and S. boulardii production.
33735774	3	46	dep	condition	633:641	arg1	obtain					646:651	obtain	646:651	to obtain the prebiotic	643:665	The best condition to obtain the prebiotic was at 32 °C and enzyme concentration of 0.175% (w/w), providing 56.84 g L-1 of GOS concentration and Ln(3.59) 107 viable cells mL-1 of S. boulardii production.
33735774	5	47	theme	functional	1179:1188	arg1	food					1190:1193	functional food	1179:1193	functional food	1179:1193	The simultaneous enzymatic-fermentative method showed promising results considering industrial application and can be easily incorporated into dairy production lines as functional food.
32679325	4	0	theme	alginate	698:705	arg1	matrix					707:712	the alginate matrix	694:712	the alginate matrix	694:712	The morphological test showed that the CuSNP were evenly distributed in the alginate matrix.
32679325	5	1	theme	hydrophobicity	773:786	arg1	properties					834:843	the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties	757:843	the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film	757:870	The addition of 0.5 wt% of CuSNP improved the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film; however, the thermal stability was not significantly influenced.
32679325	1	2	theme	Alginate-based	150:163	arg1	films					190:194	Alginate-based functional nanocomposite films	150:194	Alginate-based functional nanocomposite films	150:194	Alginate-based functional nanocomposite films were prepared by the incorporation of copper sulfide nanoparticles (CuSNP) using a solution casting method and characterized their properties.
32679325	6	3	theme	Gram-negative	1026:1038	arg1	bacteria					1050:1057	the Gram-negative (E. coli) bacteria	1022:1057	the Gram-negative (E. coli) bacteria	1022:1057	The alginate/CuSNP nanocomposite film showed evident antibacterial activity against the Gram-negative (E. coli) bacteria, but only slightly slowed the growth rate of the Garm-positive (L. monocytogenes) bacteria.
32679325	5	4	theme	%	737:737	arg1	addition					719:726	The addition	715:726	The addition of 0.5 wt% of CuSNP	715:746	The addition of 0.5 wt% of CuSNP improved the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film; however, the thermal stability was not significantly influenced.
32679325	0	5	from	Effect	0:5	arg1	barrier					59:65	water vapor barrier	47:65	water vapor barrier	47:65	Effect of CuS reinforcement on the mechanical, water vapor barrier, UV-light barrier, and antibacterial properties of alginate-based composite films.
32679325	0	5	from	Effect	0:5	arg1	barrier					77:83	UV-light barrier	68:83	UV-light barrier	68:83	Effect of CuS reinforcement on the mechanical, water vapor barrier, UV-light barrier, and antibacterial properties of alginate-based composite films.
32679325	0	5	from	Effect	0:5	arg1	properties					104:113	antibacterial properties	90:113	antibacterial properties	90:113	Effect of CuS reinforcement on the mechanical, water vapor barrier, UV-light barrier, and antibacterial properties of alginate-based composite films.
32679325	3	6	theme	composite	524:532	arg1	film					534:537	the alginate/CuSNP composite film	505:537	the alginate/CuSNP composite film	505:537	The antibacterial activity of the alginate/CuSNP composite film was evaluated against foodborne pathogenic bacteria, E. coli and L. monocytogenes.
32679325	5	7	theme	thermal	886:892	arg1	stability					894:902	the thermal stability	882:902	the thermal stability	882:902	The addition of 0.5 wt% of CuSNP improved the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film; however, the thermal stability was not significantly influenced.
32679325	0	8	theme	UV-light	68:75	arg1	barrier					77:83	UV-light barrier	68:83	UV-light barrier	68:83	Effect of CuS reinforcement on the mechanical, water vapor barrier, UV-light barrier, and antibacterial properties of alginate-based composite films.
32679325	7	9	theme	active	1279:1284	arg1	applications					1301:1312	active food packaging applications	1279:1312	active food packaging applications	1279:1312	The alginate/CuSNP nanocomposite films with significantly improved physical and functional properties have a high potential for active food packaging applications.
32679325	3	10	theme	foodborne	561:569	arg1	bacteria					582:589	foodborne pathogenic bacteria	561:589	foodborne pathogenic bacteria	561:589	The antibacterial activity of the alginate/CuSNP composite film was evaluated against foodborne pathogenic bacteria, E. coli and L. monocytogenes.
32679325	3	10	theme	foodborne	561:569	arg1	coli					595:598	E. coli	592:598	E. coli	592:598	The antibacterial activity of the alginate/CuSNP composite film was evaluated against foodborne pathogenic bacteria, E. coli and L. monocytogenes.
32679325	3	10	theme	foodborne	561:569	arg1	monocytogenes					607:619	L. monocytogenes	604:619	L. monocytogenes	604:619	The antibacterial activity of the alginate/CuSNP composite film was evaluated against foodborne pathogenic bacteria, E. coli and L. monocytogenes.
32679325	3	11	theme	antibacterial	479:491	arg1	activity					493:500	The antibacterial activity	475:500	The antibacterial activity of the alginate/CuSNP composite film	475:537	The antibacterial activity of the alginate/CuSNP composite film was evaluated against foodborne pathogenic bacteria, E. coli and L. monocytogenes.
32679325	5	12	theme	CuSNP	742:746	arg1	CuSNP					742:746	CuSNP	742:746	CuSNP	742:746	The addition of 0.5 wt% of CuSNP improved the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film; however, the thermal stability was not significantly influenced.
32679325	5	12	theme	CuSNP	742:746	arg1	%					737:737	0.5 wt%	731:737	0.5 wt% of CuSNP	731:746	The addition of 0.5 wt% of CuSNP improved the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film; however, the thermal stability was not significantly influenced.
32679325	1	13	theme	functional	165:174	arg1	films					190:194	Alginate-based functional nanocomposite films	150:194	Alginate-based functional nanocomposite films	150:194	Alginate-based functional nanocomposite films were prepared by the incorporation of copper sulfide nanoparticles (CuSNP) using a solution casting method and characterized their properties.
32679325	2	14	theme	films	456:460	arg1	surface					385:391	surface wetting	385:399	surface wetting	385:399	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	2	14	theme	films	456:460	arg1	properties					424:433	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties	339:433	properties	424:433	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	2	14	theme	films	456:460	arg1	barrier					376:382	water vapor barrier	364:382	water vapor barrier	364:382	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	2	14	theme	films	456:460	arg1	stability					414:422	thermal stability	406:422	thermal stability	406:422	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	6	15	dep	bacteria	1050:1057	arg1	coli					1044:1047	E. coli	1041:1047	E. coli	1041:1047	The alginate/CuSNP nanocomposite film showed evident antibacterial activity against the Gram-negative (E. coli) bacteria, but only slightly slowed the growth rate of the Garm-positive (L. monocytogenes) bacteria.
32679325	2	16	dep	properties	424:433	arg1	surface					385:391	surface wetting	385:399	surface wetting	385:399	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	2	16	dep	properties	424:433	arg1	barrier					376:382	water vapor barrier	364:382	water vapor barrier	364:382	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	2	16	dep	properties	424:433	arg1	properties					424:433	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties	339:433	properties	424:433	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	2	16	dep	properties	424:433	arg1	stability					414:422	thermal stability	406:422	thermal stability	406:422	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	7	17	theme	functional	1231:1240	arg1	properties					1242:1251	significantly improved physical and functional properties	1195:1251	significantly improved physical and functional properties	1195:1251	The alginate/CuSNP nanocomposite films with significantly improved physical and functional properties have a high potential for active food packaging applications.
32679325	7	18	theme	improved	1209:1216	arg1	properties					1242:1251	significantly improved physical and functional properties	1195:1251	significantly improved physical and functional properties	1195:1251	The alginate/CuSNP nanocomposite films with significantly improved physical and functional properties have a high potential for active food packaging applications.
32679325	1	19	theme	nanocomposite	176:188	arg1	films					190:194	Alginate-based functional nanocomposite films	150:194	Alginate-based functional nanocomposite films	150:194	Alginate-based functional nanocomposite films were prepared by the incorporation of copper sulfide nanoparticles (CuSNP) using a solution casting method and characterized their properties.
32679325	2	20	theme	nanocomposite	442:454	arg1	films					456:460	the nanocomposite films	438:460	the nanocomposite films	438:460	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	7	21	theme	high	1260:1263	arg1	potential					1265:1273	a high potential	1258:1273	a high potential for active food packaging applications	1258:1312	The alginate/CuSNP nanocomposite films with significantly improved physical and functional properties have a high potential for active food packaging applications.
32679325	7	22	with	films	1184:1188	arg1	properties					1242:1251	significantly improved physical and functional properties	1195:1251	significantly improved physical and functional properties	1195:1251	The alginate/CuSNP nanocomposite films with significantly improved physical and functional properties have a high potential for active food packaging applications.
32679325	6	23	theme	evident	983:989	arg1	activity					1005:1012	evident antibacterial activity	983:1012	evident antibacterial activity against the Gram-negative (E. coli) bacteria	983:1057	The alginate/CuSNP nanocomposite film showed evident antibacterial activity against the Gram-negative (E. coli) bacteria, but only slightly slowed the growth rate of the Garm-positive (L. monocytogenes) bacteria.
32679325	5	24	theme	water	814:818	arg1	vapor					820:824	water vapor	814:824	water vapor	814:824	The addition of 0.5 wt% of CuSNP improved the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film; however, the thermal stability was not significantly influenced.
32679325	7	25	theme	nanocomposite	1170:1182	arg1	films					1184:1188	The alginate/CuSNP nanocomposite films	1151:1188	The alginate/CuSNP nanocomposite films with significantly improved physical and functional properties	1151:1251	The alginate/CuSNP nanocomposite films with significantly improved physical and functional properties have a high potential for active food packaging applications.
32679325	5	26	theme	film	867:870	arg1	properties					834:843	the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties	757:843	the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film	757:870	The addition of 0.5 wt% of CuSNP improved the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film; however, the thermal stability was not significantly influenced.
32679325	0	27	dep	barrier	59:65	arg1	the					31:33	the	31:33	the	31:33	Effect of CuS reinforcement on the mechanical, water vapor barrier, UV-light barrier, and antibacterial properties of alginate-based composite films.
32679325	0	28	theme	reinforcement	14:26	arg1	Effect					0:5	Effect	0:5	Effect of CuS reinforcement on the mechanical, water vapor barrier, UV-light barrier, and antibacterial properties of alginate-based composite films.	0:148	Effect of CuS reinforcement on the mechanical, water vapor barrier, UV-light barrier, and antibacterial properties of alginate-based composite films.
32679325	1	29	theme	solution	279:286	arg1	method					296:301	a solution casting method	277:301	a solution casting method	277:301	Alginate-based functional nanocomposite films were prepared by the incorporation of copper sulfide nanoparticles (CuSNP) using a solution casting method and characterized their properties.
32679325	3	30	theme	pathogenic	571:580	arg1	bacteria					582:589	foodborne pathogenic bacteria	561:589	foodborne pathogenic bacteria	561:589	The antibacterial activity of the alginate/CuSNP composite film was evaluated against foodborne pathogenic bacteria, E. coli and L. monocytogenes.
32679325	3	30	theme	pathogenic	571:580	arg1	coli					595:598	E. coli	592:598	E. coli	592:598	The antibacterial activity of the alginate/CuSNP composite film was evaluated against foodborne pathogenic bacteria, E. coli and L. monocytogenes.
32679325	3	30	theme	pathogenic	571:580	arg1	monocytogenes					607:619	L. monocytogenes	604:619	L. monocytogenes	604:619	The antibacterial activity of the alginate/CuSNP composite film was evaluated against foodborne pathogenic bacteria, E. coli and L. monocytogenes.
32679325	0	31	theme	CuS	10:12	arg1	reinforcement					14:26	CuS reinforcement	10:26	CuS reinforcement	10:26	Effect of CuS reinforcement on the mechanical, water vapor barrier, UV-light barrier, and antibacterial properties of alginate-based composite films.
32679325	1	32	theme	casting	288:294	arg1	method					296:301	a solution casting method	277:301	a solution casting method	277:301	Alginate-based functional nanocomposite films were prepared by the incorporation of copper sulfide nanoparticles (CuSNP) using a solution casting method and characterized their properties.
32679325	0	33	theme	antibacterial	90:102	arg1	properties					104:113	antibacterial properties	90:113	antibacterial properties	90:113	Effect of CuS reinforcement on the mechanical, water vapor barrier, UV-light barrier, and antibacterial properties of alginate-based composite films.
32679325	2	34	dep	optical	343:349	arg1	mechanical					352:361	mechanical	352:361	mechanical	352:361	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	2	35	theme	thermal	406:412	arg1	properties					424:433	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties	339:433	properties	424:433	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	2	35	theme	thermal	406:412	arg1	stability					414:422	thermal stability	406:422	thermal stability	406:422	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	7	36	theme	alginate/CuSNP	1155:1168	arg1	films					1184:1188	The alginate/CuSNP nanocomposite films	1151:1188	The alginate/CuSNP nanocomposite films with significantly improved physical and functional properties	1151:1251	The alginate/CuSNP nanocomposite films with significantly improved physical and functional properties have a high potential for active food packaging applications.
32679325	0	37	theme	alginate-based	118:131	arg1	films					143:147	alginate-based composite films	118:147	alginate-based composite films	118:147	Effect of CuS reinforcement on the mechanical, water vapor barrier, UV-light barrier, and antibacterial properties of alginate-based composite films.
32679325	7	38	theme	food	1286:1289	arg1	applications					1301:1312	active food packaging applications	1279:1312	active food packaging applications	1279:1312	The alginate/CuSNP nanocomposite films with significantly improved physical and functional properties have a high potential for active food packaging applications.
32679325	2	39	theme	wetting	393:399	arg1	surface					385:391	surface wetting	385:399	surface wetting	385:399	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	2	39	theme	wetting	393:399	arg1	properties					424:433	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties	339:433	properties	424:433	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	5	40	theme	0.5 wt	731:736	arg1	CuSNP					742:746	CuSNP	742:746	CuSNP	742:746	The addition of 0.5 wt% of CuSNP improved the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film; however, the thermal stability was not significantly influenced.
32679325	5	40	theme	0.5 wt	731:736	arg1	%					737:737	0.5 wt%	731:737	0.5 wt% of CuSNP	731:746	The addition of 0.5 wt% of CuSNP improved the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film; however, the thermal stability was not significantly influenced.
32679325	5	41	theme	vapor	820:824	arg1	properties					834:843	the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties	757:843	the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film	757:870	The addition of 0.5 wt% of CuSNP improved the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film; however, the thermal stability was not significantly influenced.
32679325	5	42	theme	alginate-based	852:865	arg1	film					867:870	the alginate-based film	848:870	the alginate-based film	848:870	The addition of 0.5 wt% of CuSNP improved the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film; however, the thermal stability was not significantly influenced.
32679325	5	43	theme	strength	800:807	arg1	properties					834:843	the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties	757:843	the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film	757:870	The addition of 0.5 wt% of CuSNP improved the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film; however, the thermal stability was not significantly influenced.
32679325	6	44	theme	nanocomposite	957:969	arg1	film					971:974	The alginate/CuSNP nanocomposite film	938:974	The alginate/CuSNP nanocomposite film	938:974	The alginate/CuSNP nanocomposite film showed evident antibacterial activity against the Gram-negative (E. coli) bacteria, but only slightly slowed the growth rate of the Garm-positive (L. monocytogenes) bacteria.
32679325	0	45	theme	films	143:147	arg1	barrier					59:65	water vapor barrier	47:65	water vapor barrier	47:65	Effect of CuS reinforcement on the mechanical, water vapor barrier, UV-light barrier, and antibacterial properties of alginate-based composite films.
32679325	0	45	theme	films	143:147	arg1	barrier					77:83	UV-light barrier	68:83	UV-light barrier	68:83	Effect of CuS reinforcement on the mechanical, water vapor barrier, UV-light barrier, and antibacterial properties of alginate-based composite films.
32679325	0	45	theme	films	143:147	arg1	properties					104:113	antibacterial properties	90:113	antibacterial properties	90:113	Effect of CuS reinforcement on the mechanical, water vapor barrier, UV-light barrier, and antibacterial properties of alginate-based composite films.
32679325	5	46	theme	UV-barrier	761:770	arg1	properties					834:843	the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties	757:843	the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film	757:870	The addition of 0.5 wt% of CuSNP improved the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film; however, the thermal stability was not significantly influenced.
32679325	6	47	theme	bacteria	1141:1148	arg1	rate					1096:1099	the growth rate	1085:1099	the growth rate of the Garm-positive (L. monocytogenes) bacteria	1085:1148	The alginate/CuSNP nanocomposite film showed evident antibacterial activity against the Gram-negative (E. coli) bacteria, but only slightly slowed the growth rate of the Garm-positive (L. monocytogenes) bacteria.
32679325	0	48	theme	mechanical	35:44	arg1	barrier					59:65	water vapor barrier	47:65	water vapor barrier	47:65	Effect of CuS reinforcement on the mechanical, water vapor barrier, UV-light barrier, and antibacterial properties of alginate-based composite films.
32679325	6	49	theme	alginate/CuSNP	942:955	arg1	film					971:974	The alginate/CuSNP nanocomposite film	938:974	The alginate/CuSNP nanocomposite film	938:974	The alginate/CuSNP nanocomposite film showed evident antibacterial activity against the Gram-negative (E. coli) bacteria, but only slightly slowed the growth rate of the Garm-positive (L. monocytogenes) bacteria.
32679325	0	50	theme	composite	133:141	arg1	films					143:147	alginate-based composite films	118:147	alginate-based composite films	118:147	Effect of CuS reinforcement on the mechanical, water vapor barrier, UV-light barrier, and antibacterial properties of alginate-based composite films.
32679325	3	51	theme	alginate/CuSNP	509:522	arg1	film					534:537	the alginate/CuSNP composite film	505:537	the alginate/CuSNP composite film	505:537	The antibacterial activity of the alginate/CuSNP composite film was evaluated against foodborne pathogenic bacteria, E. coli and L. monocytogenes.
32679325	2	52	theme	optical	343:349	arg1	surface					385:391	surface wetting	385:399	surface wetting	385:399	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	2	52	theme	optical	343:349	arg1	properties					424:433	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties	339:433	properties	424:433	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	2	52	theme	optical	343:349	arg1	barrier					376:382	water vapor barrier	364:382	water vapor barrier	364:382	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	2	52	theme	optical	343:349	arg1	stability					414:422	thermal stability	406:422	thermal stability	406:422	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	7	53	theme	packaging	1291:1299	arg1	applications					1301:1312	active food packaging applications	1279:1312	active food packaging applications	1279:1312	The alginate/CuSNP nanocomposite films with significantly improved physical and functional properties have a high potential for active food packaging applications.
32679325	5	54	theme	mechanical	789:798	arg1	strength					800:807	mechanical strength	789:807	mechanical strength	789:807	The addition of 0.5 wt% of CuSNP improved the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film; however, the thermal stability was not significantly influenced.
32679325	5	55	theme	barrier	826:832	arg1	properties					834:843	the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties	757:843	the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film	757:870	The addition of 0.5 wt% of CuSNP improved the UV-barrier, hydrophobicity, mechanical strength, and water vapor barrier properties of the alginate-based film; however, the thermal stability was not significantly influenced.
32679325	3	56	theme	film	534:537	arg1	activity					493:500	The antibacterial activity	475:500	The antibacterial activity of the alginate/CuSNP composite film	475:537	The antibacterial activity of the alginate/CuSNP composite film was evaluated against foodborne pathogenic bacteria, E. coli and L. monocytogenes.
32679325	6	57	theme	antibacterial	991:1003	arg1	activity					1005:1012	evident antibacterial activity	983:1012	evident antibacterial activity against the Gram-negative (E. coli) bacteria	983:1057	The alginate/CuSNP nanocomposite film showed evident antibacterial activity against the Gram-negative (E. coli) bacteria, but only slightly slowed the growth rate of the Garm-positive (L. monocytogenes) bacteria.
32679325	1	58	theme	copper	234:239	arg1	CuSNP					264:268	CuSNP	264:268	CuSNP	264:268	Alginate-based functional nanocomposite films were prepared by the incorporation of copper sulfide nanoparticles (CuSNP) using a solution casting method and characterized their properties.
32679325	1	58	theme	copper	234:239	arg1	nanoparticles					249:261	copper sulfide nanoparticles	234:261	copper sulfide nanoparticles (CuSNP) using a solution casting method	234:301	Alginate-based functional nanocomposite films were prepared by the incorporation of copper sulfide nanoparticles (CuSNP) using a solution casting method and characterized their properties.
32679325	0	59	theme	vapor	53:57	arg1	barrier					59:65	water vapor barrier	47:65	water vapor barrier	47:65	Effect of CuS reinforcement on the mechanical, water vapor barrier, UV-light barrier, and antibacterial properties of alginate-based composite films.
32679325	6	60	theme	Garm-positive	1108:1120	arg1	bacteria					1141:1148	the Garm-positive (L. monocytogenes) bacteria	1104:1148	the Garm-positive (L. monocytogenes) bacteria	1104:1148	The alginate/CuSNP nanocomposite film showed evident antibacterial activity against the Gram-negative (E. coli) bacteria, but only slightly slowed the growth rate of the Garm-positive (L. monocytogenes) bacteria.
32679325	6	61	dep	bacteria	1141:1148	arg1	monocytogenes					1126:1138	L. monocytogenes	1123:1138	L. monocytogenes	1123:1138	The alginate/CuSNP nanocomposite film showed evident antibacterial activity against the Gram-negative (E. coli) bacteria, but only slightly slowed the growth rate of the Garm-positive (L. monocytogenes) bacteria.
32679325	7	62	contain	have	1253:1256	arg2	potential					1265:1273	a high potential	1258:1273	a high potential for active food packaging applications	1258:1312	The alginate/CuSNP nanocomposite films with significantly improved physical and functional properties have a high potential for active food packaging applications.
32679325	7	62	contain	have	1253:1256	arg1	films					1184:1188	The alginate/CuSNP nanocomposite films	1151:1188	The alginate/CuSNP nanocomposite films with significantly improved physical and functional properties	1151:1251	The alginate/CuSNP nanocomposite films with significantly improved physical and functional properties have a high potential for active food packaging applications.
32679325	2	63	theme	vapor	370:374	arg1	barrier					376:382	water vapor barrier	364:382	water vapor barrier	364:382	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	2	63	theme	vapor	370:374	arg1	properties					424:433	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties	339:433	properties	424:433	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	6	64	theme	growth	1089:1094	arg1	rate					1096:1099	the growth rate	1085:1099	the growth rate of the Garm-positive (L. monocytogenes) bacteria	1085:1148	The alginate/CuSNP nanocomposite film showed evident antibacterial activity against the Gram-negative (E. coli) bacteria, but only slightly slowed the growth rate of the Garm-positive (L. monocytogenes) bacteria.
32679325	1	65	theme	sulfide	241:247	arg1	CuSNP					264:268	CuSNP	264:268	CuSNP	264:268	Alginate-based functional nanocomposite films were prepared by the incorporation of copper sulfide nanoparticles (CuSNP) using a solution casting method and characterized their properties.
32679325	1	65	theme	sulfide	241:247	arg1	nanoparticles					249:261	copper sulfide nanoparticles	234:261	copper sulfide nanoparticles (CuSNP) using a solution casting method	234:301	Alginate-based functional nanocomposite films were prepared by the incorporation of copper sulfide nanoparticles (CuSNP) using a solution casting method and characterized their properties.
32679325	0	66	theme	water	47:51	arg1	barrier					59:65	water vapor barrier	47:65	water vapor barrier	47:65	Effect of CuS reinforcement on the mechanical, water vapor barrier, UV-light barrier, and antibacterial properties of alginate-based composite films.
32679325	7	67	theme	physical	1218:1225	arg1	properties					1242:1251	significantly improved physical and functional properties	1195:1251	significantly improved physical and functional properties	1195:1251	The alginate/CuSNP nanocomposite films with significantly improved physical and functional properties have a high potential for active food packaging applications.
32679325	4	68	theme	morphological	626:638	arg1	test					640:643	The morphological test	622:643	The morphological test	622:643	The morphological test showed that the CuSNP were evenly distributed in the alginate matrix.
32679325	2	69	theme	water	364:368	arg1	barrier					376:382	water vapor barrier	364:382	water vapor barrier	364:382	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	2	69	theme	water	364:368	arg1	properties					424:433	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties	339:433	properties	424:433	The optical, mechanical, water vapor barrier, surface wetting, and thermal stability properties of the nanocomposite films were tested.
32679325	1	70	theme	nanoparticles	249:261	arg1	incorporation					217:229	the incorporation	213:229	the incorporation of copper sulfide nanoparticles (CuSNP) using a solution casting method	213:301	Alginate-based functional nanocomposite films were prepared by the incorporation of copper sulfide nanoparticles (CuSNP) using a solution casting method and characterized their properties.
34560966	3	0	theme	sequences	371:379	arg1	modifications					332:344	the process-related modifications	312:344	the process-related modifications of internal and terminal sequences of enoxaparin	312:393	While the process-related modifications of internal and terminal sequences of enoxaparin have been extensively studied, very little is known about the authentic non-reducing ends (NRE).
34560966	2	1	theme	used	124:127	arg1	drug					144:147	widely used antithrombotic drug	117:147	widely used antithrombotic drug	117:147	Enoxaparin, widely used antithrombotic drug, is a polydisperse glycosaminoglycan with highly microheterogeneous structure dictated by both parent heparin heterogeneity and depolymerization conditions.
34560966	2	1	theme	used	124:127	arg1	Enoxaparin					105:114	Enoxaparin	105:114	Enoxaparin	105:114	Enoxaparin, widely used antithrombotic drug, is a polydisperse glycosaminoglycan with highly microheterogeneous structure dictated by both parent heparin heterogeneity and depolymerization conditions.
34560966	6	2	theme	glucuronic	950:959	arg1	acids					974:978	both glucuronic and iduronic acids	945:978	both glucuronic and iduronic acids	945:978	In particular, both glucuronic and iduronic acids were detected at the NRE of macromolecular heparin.
34560966	7	3	theme	heparanase	1120:1129	arg1	action					1142:1147	the heparanase hydrolytic action	1116:1147	the heparanase hydrolytic action	1116:1147	The tetrasaccharides bearing glucosamine at the NRE are most likely associated with the heparanase hydrolytic action.
34560966	8	4	theme	sulfation	1155:1163	arg1	degree					1165:1170	High sulfation degree	1150:1170	High sulfation degree	1150:1170	High sulfation degree and 3-O-sulfation are characteristic for both types of NRE.
34560966	6	5	located	detected	985:992	arg1	particular					933:942	particular	933:942	particular	933:942	In particular, both glucuronic and iduronic acids were detected at the NRE of macromolecular heparin.
34560966	6	5	located	detected	985:992	arg1	NRE					1001:1003	the NRE	997:1003	the NRE of macromolecular heparin	997:1029	In particular, both glucuronic and iduronic acids were detected at the NRE of macromolecular heparin.
34560966	6	5	located	detected	985:992	arg2	acids					974:978	both glucuronic and iduronic acids	945:978	both glucuronic and iduronic acids	945:978	In particular, both glucuronic and iduronic acids were detected at the NRE of macromolecular heparin.
34560966	4	6	theme	saturated	612:620	arg1	tetramers					622:630	16 saturated tetramers	609:630	16 saturated tetramers along with 23 unsaturated ones in the complex enoxaparin tetrasaccharide fraction	609:712	In the present study a multi-step isolation and thorough structural elucidation by NMR and LC/MS allowed to identify 16 saturated tetramers along with 23 unsaturated ones in the complex enoxaparin tetrasaccharide fraction.
34560966	5	7	dep	represent	752:760	arg1	whereas					793:799	whereas	793:799	whereas	793:799	Altogether the elucidated structures represent a unique enoxaparin signature, whereas the composition of saturated tetramers provides a structural readout strictly related to the biosynthesis of parent heparin NRE.
34560966	4	8	theme	structural	549:558	arg1	elucidation					560:570	thorough structural elucidation	540:570	thorough structural elucidation	540:570	In the present study a multi-step isolation and thorough structural elucidation by NMR and LC/MS allowed to identify 16 saturated tetramers along with 23 unsaturated ones in the complex enoxaparin tetrasaccharide fraction.
34560966	4	9	theme	thorough	540:547	arg1	elucidation					560:570	thorough structural elucidation	540:570	thorough structural elucidation	540:570	In the present study a multi-step isolation and thorough structural elucidation by NMR and LC/MS allowed to identify 16 saturated tetramers along with 23 unsaturated ones in the complex enoxaparin tetrasaccharide fraction.
34560966	5	10	theme	structural	851:860	arg1	readout					862:868	a structural readout	849:868	a structural readout strictly related to the biosynthesis of parent heparin NRE	849:927	Altogether the elucidated structures represent a unique enoxaparin signature, whereas the composition of saturated tetramers provides a structural readout strictly related to the biosynthesis of parent heparin NRE.
34560966	1	11	theme	heparin	76:82	arg1	puzzle					97:102	the heparin biosynthesis puzzle	72:102	the heparin biosynthesis puzzle	72:102	An additional piece to the heparin biosynthesis puzzle.
34560966	0	12	theme	tetrasaccharide	10:24	arg1	profile					26:32	Saturated tetrasaccharide profile	0:32	Saturated tetrasaccharide profile of enoxaparin	0:46	Saturated tetrasaccharide profile of enoxaparin.
34560966	4	13	theme	multi-step	515:524	arg1	isolation					526:534	a multi-step isolation	513:534	a multi-step isolation	513:534	In the present study a multi-step isolation and thorough structural elucidation by NMR and LC/MS allowed to identify 16 saturated tetramers along with 23 unsaturated ones in the complex enoxaparin tetrasaccharide fraction.
34560966	7	14	theme	hydrolytic	1131:1140	arg1	action					1142:1147	the heparanase hydrolytic action	1116:1147	the heparanase hydrolytic action	1116:1147	The tetrasaccharides bearing glucosamine at the NRE are most likely associated with the heparanase hydrolytic action.
34560966	5	15	theme	saturated	820:828	arg1	tetramers					830:838	saturated tetramers	820:838	saturated tetramers	820:838	Altogether the elucidated structures represent a unique enoxaparin signature, whereas the composition of saturated tetramers provides a structural readout strictly related to the biosynthesis of parent heparin NRE.
34560966	1	16	theme	biosynthesis	84:95	arg1	puzzle					97:102	the heparin biosynthesis puzzle	72:102	the heparin biosynthesis puzzle	72:102	An additional piece to the heparin biosynthesis puzzle.
34560966	6	17	theme	iduronic	965:972	arg1	acids					974:978	both glucuronic and iduronic acids	945:978	both glucuronic and iduronic acids	945:978	In particular, both glucuronic and iduronic acids were detected at the NRE of macromolecular heparin.
34560966	0	18	theme	Saturated	0:8	arg1	profile					26:32	Saturated tetrasaccharide profile	0:32	Saturated tetrasaccharide profile of enoxaparin	0:46	Saturated tetrasaccharide profile of enoxaparin.
34560966	8	19	theme	High	1150:1153	arg1	degree					1165:1170	High sulfation degree	1150:1170	High sulfation degree	1150:1170	High sulfation degree and 3-O-sulfation are characteristic for both types of NRE.
34560966	4	20	dep	along	632:636	arg1	with					638:641	with	638:641	with	638:641	In the present study a multi-step isolation and thorough structural elucidation by NMR and LC/MS allowed to identify 16 saturated tetramers along with 23 unsaturated ones in the complex enoxaparin tetrasaccharide fraction.
34560966	5	21	theme	NRE	925:927	arg1	biosynthesis					894:905	the biosynthesis	890:905	the biosynthesis of parent heparin NRE	890:927	Altogether the elucidated structures represent a unique enoxaparin signature, whereas the composition of saturated tetramers provides a structural readout strictly related to the biosynthesis of parent heparin NRE.
34560966	3	22	theme	process-related	316:330	arg1	modifications					332:344	the process-related modifications	312:344	the process-related modifications of internal and terminal sequences of enoxaparin	312:393	While the process-related modifications of internal and terminal sequences of enoxaparin have been extensively studied, very little is known about the authentic non-reducing ends (NRE).
34560966	4	23	theme	complex	670:676	arg1	fraction					705:712	the complex enoxaparin tetrasaccharide fraction	666:712	the complex enoxaparin tetrasaccharide fraction	666:712	In the present study a multi-step isolation and thorough structural elucidation by NMR and LC/MS allowed to identify 16 saturated tetramers along with 23 unsaturated ones in the complex enoxaparin tetrasaccharide fraction.
34560966	8	24	theme	NRE	1227:1229	arg1	types					1218:1222	both types	1213:1222	both types of NRE	1213:1229	High sulfation degree and 3-O-sulfation are characteristic for both types of NRE.
34560966	5	25	theme	enoxaparin	771:780	arg1	signature					782:790	a unique enoxaparin signature	762:790	a unique enoxaparin signature	762:790	Altogether the elucidated structures represent a unique enoxaparin signature, whereas the composition of saturated tetramers provides a structural readout strictly related to the biosynthesis of parent heparin NRE.
34560966	5	26	theme	heparin	917:923	arg1	NRE					925:927	parent heparin NRE	910:927	parent heparin NRE	910:927	Altogether the elucidated structures represent a unique enoxaparin signature, whereas the composition of saturated tetramers provides a structural readout strictly related to the biosynthesis of parent heparin NRE.
34560966	3	27	theme	authentic	457:465	arg1	NRE					486:488	NRE	486:488	NRE	486:488	While the process-related modifications of internal and terminal sequences of enoxaparin have been extensively studied, very little is known about the authentic non-reducing ends (NRE).
34560966	3	27	theme	authentic	457:465	arg1	ends					480:483	the authentic non-reducing ends	453:483	the authentic non-reducing ends (NRE)	453:489	While the process-related modifications of internal and terminal sequences of enoxaparin have been extensively studied, very little is known about the authentic non-reducing ends (NRE).
34560966	4	28	theme	tetrasaccharide	689:703	arg1	fraction					705:712	the complex enoxaparin tetrasaccharide fraction	666:712	the complex enoxaparin tetrasaccharide fraction	666:712	In the present study a multi-step isolation and thorough structural elucidation by NMR and LC/MS allowed to identify 16 saturated tetramers along with 23 unsaturated ones in the complex enoxaparin tetrasaccharide fraction.
34560966	2	29	dep	heterogeneity	259:271	arg1	conditions					294:303	conditions	294:303	conditions	294:303	Enoxaparin, widely used antithrombotic drug, is a polydisperse glycosaminoglycan with highly microheterogeneous structure dictated by both parent heparin heterogeneity and depolymerization conditions.
34560966	0	30	theme	enoxaparin	37:46	arg1	profile					26:32	Saturated tetrasaccharide profile	0:32	Saturated tetrasaccharide profile of enoxaparin	0:46	Saturated tetrasaccharide profile of enoxaparin.
34560966	6	31	theme	heparin	1023:1029	arg1	NRE					1001:1003	the NRE	997:1003	the NRE of macromolecular heparin	997:1029	In particular, both glucuronic and iduronic acids were detected at the NRE of macromolecular heparin.
34560966	3	32	theme	non-reducing	467:478	arg1	NRE					486:488	NRE	486:488	NRE	486:488	While the process-related modifications of internal and terminal sequences of enoxaparin have been extensively studied, very little is known about the authentic non-reducing ends (NRE).
34560966	3	32	theme	non-reducing	467:478	arg1	ends					480:483	the authentic non-reducing ends	453:483	the authentic non-reducing ends (NRE)	453:489	While the process-related modifications of internal and terminal sequences of enoxaparin have been extensively studied, very little is known about the authentic non-reducing ends (NRE).
34560966	4	33	theme	present	499:505	arg1	study					507:511	the present study	495:511	the present study	495:511	In the present study a multi-step isolation and thorough structural elucidation by NMR and LC/MS allowed to identify 16 saturated tetramers along with 23 unsaturated ones in the complex enoxaparin tetrasaccharide fraction.
34560966	2	34	theme	heparin	251:257	arg1	heterogeneity					259:271	parent heparin heterogeneity	244:271	parent heparin heterogeneity	244:271	Enoxaparin, widely used antithrombotic drug, is a polydisperse glycosaminoglycan with highly microheterogeneous structure dictated by both parent heparin heterogeneity and depolymerization conditions.
34560966	4	35	theme	enoxaparin	678:687	arg1	fraction					705:712	the complex enoxaparin tetrasaccharide fraction	666:712	the complex enoxaparin tetrasaccharide fraction	666:712	In the present study a multi-step isolation and thorough structural elucidation by NMR and LC/MS allowed to identify 16 saturated tetramers along with 23 unsaturated ones in the complex enoxaparin tetrasaccharide fraction.
34560966	6	36	theme	macromolecular	1008:1021	arg1	heparin					1023:1029	macromolecular heparin	1008:1029	macromolecular heparin	1008:1029	In particular, both glucuronic and iduronic acids were detected at the NRE of macromolecular heparin.
34560966	5	37	theme	elucidated	730:739	arg1	structures					741:750	the elucidated structures	726:750	the elucidated structures	726:750	Altogether the elucidated structures represent a unique enoxaparin signature, whereas the composition of saturated tetramers provides a structural readout strictly related to the biosynthesis of parent heparin NRE.
34560966	2	38	theme	polydisperse	155:166	arg1	glycosaminoglycan					168:184	a polydisperse glycosaminoglycan	153:184	a polydisperse glycosaminoglycan with highly microheterogeneous structure dictated by both parent heparin heterogeneity and depolymerization conditions	153:303	Enoxaparin, widely used antithrombotic drug, is a polydisperse glycosaminoglycan with highly microheterogeneous structure dictated by both parent heparin heterogeneity and depolymerization conditions.
34560966	2	38	theme	polydisperse	155:166	arg1	Enoxaparin					105:114	Enoxaparin	105:114	Enoxaparin	105:114	Enoxaparin, widely used antithrombotic drug, is a polydisperse glycosaminoglycan with highly microheterogeneous structure dictated by both parent heparin heterogeneity and depolymerization conditions.
34560966	2	39	theme	parent	244:249	arg1	heterogeneity					259:271	parent heparin heterogeneity	244:271	parent heparin heterogeneity	244:271	Enoxaparin, widely used antithrombotic drug, is a polydisperse glycosaminoglycan with highly microheterogeneous structure dictated by both parent heparin heterogeneity and depolymerization conditions.
34560966	5	40	theme	related	879:885	arg1	readout					862:868	a structural readout	849:868	a structural readout strictly related to the biosynthesis of parent heparin NRE	849:927	Altogether the elucidated structures represent a unique enoxaparin signature, whereas the composition of saturated tetramers provides a structural readout strictly related to the biosynthesis of parent heparin NRE.
34560966	3	41	theme	terminal	362:369	arg1	sequences					371:379	internal and terminal sequences	349:379	internal and terminal sequences of enoxaparin	349:393	While the process-related modifications of internal and terminal sequences of enoxaparin have been extensively studied, very little is known about the authentic non-reducing ends (NRE).
34560966	5	42	theme	unique	764:769	arg1	signature					782:790	a unique enoxaparin signature	762:790	a unique enoxaparin signature	762:790	Altogether the elucidated structures represent a unique enoxaparin signature, whereas the composition of saturated tetramers provides a structural readout strictly related to the biosynthesis of parent heparin NRE.
34560966	5	43	theme	tetramers	830:838	arg1	composition					805:815	the composition	801:815	the composition of saturated tetramers	801:838	Altogether the elucidated structures represent a unique enoxaparin signature, whereas the composition of saturated tetramers provides a structural readout strictly related to the biosynthesis of parent heparin NRE.
34560966	4	44	theme	unsaturated	646:656	arg1	ones					658:661	23 unsaturated ones	643:661	16 saturated tetramers along with 23 unsaturated ones in the complex enoxaparin tetrasaccharide fraction	609:712	In the present study a multi-step isolation and thorough structural elucidation by NMR and LC/MS allowed to identify 16 saturated tetramers along with 23 unsaturated ones in the complex enoxaparin tetrasaccharide fraction.
34560966	3	45	theme	internal	349:356	arg1	sequences					371:379	internal and terminal sequences	349:379	internal and terminal sequences of enoxaparin	349:393	While the process-related modifications of internal and terminal sequences of enoxaparin have been extensively studied, very little is known about the authentic non-reducing ends (NRE).
34560966	3	46	theme	enoxaparin	384:393	arg1	sequences					371:379	internal and terminal sequences	349:379	internal and terminal sequences of enoxaparin	349:393	While the process-related modifications of internal and terminal sequences of enoxaparin have been extensively studied, very little is known about the authentic non-reducing ends (NRE).
34560966	2	47	theme	microheterogeneous	198:215	arg1	structure					217:225	highly microheterogeneous structure	191:225	highly microheterogeneous structure	191:225	Enoxaparin, widely used antithrombotic drug, is a polydisperse glycosaminoglycan with highly microheterogeneous structure dictated by both parent heparin heterogeneity and depolymerization conditions.
34560966	5	48	theme	parent	910:915	arg1	NRE					925:927	parent heparin NRE	910:927	parent heparin NRE	910:927	Altogether the elucidated structures represent a unique enoxaparin signature, whereas the composition of saturated tetramers provides a structural readout strictly related to the biosynthesis of parent heparin NRE.
34560966	4	49	from	ones	658:661	arg1	fraction					705:712	the complex enoxaparin tetrasaccharide fraction	666:712	the complex enoxaparin tetrasaccharide fraction	666:712	In the present study a multi-step isolation and thorough structural elucidation by NMR and LC/MS allowed to identify 16 saturated tetramers along with 23 unsaturated ones in the complex enoxaparin tetrasaccharide fraction.
34560966	2	50	theme	antithrombotic	129:142	arg1	drug					144:147	widely used antithrombotic drug	117:147	widely used antithrombotic drug	117:147	Enoxaparin, widely used antithrombotic drug, is a polydisperse glycosaminoglycan with highly microheterogeneous structure dictated by both parent heparin heterogeneity and depolymerization conditions.
34560966	2	50	theme	antithrombotic	129:142	arg1	Enoxaparin					105:114	Enoxaparin	105:114	Enoxaparin	105:114	Enoxaparin, widely used antithrombotic drug, is a polydisperse glycosaminoglycan with highly microheterogeneous structure dictated by both parent heparin heterogeneity and depolymerization conditions.
34560966	1	51	theme	additional	52:61	arg1	piece					63:67	An additional piece	49:67	An additional piece to the heparin biosynthesis puzzle	49:102	An additional piece to the heparin biosynthesis puzzle.
32902634	0	0	theme	glycoproteins	64:76	arg1	NMR					56:58	NMR	56:58	NMR	56:58	Sparse isotope labeling for nuclear magnetic resonance (NMR) of glycoproteins using 13C-glucose.
32902634	0	0	theme	glycoproteins	64:76	arg1	resonance					45:53	nuclear magnetic resonance	28:53	nuclear magnetic resonance (NMR) of glycoproteins using 13C-glucose	28:94	Sparse isotope labeling for nuclear magnetic resonance (NMR) of glycoproteins using 13C-glucose.
32902634	6	1	theme	many	1297:1300	arg1	proteins					1315:1322	the many glycosylated proteins	1293:1322	the many glycosylated proteins important to human health	1293:1348	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	3	2	theme	mammalian	486:494	arg1	culture					496:502	mammalian culture	486:502	mammalian culture where more costly isotopically enriched amino acids are usually used	486:571	However, it can be expensive for glycosylated proteins expressed in mammalian culture where more costly isotopically enriched amino acids are usually used.
32902634	4	3	gly	glycosylated	798:809	arg1	protein					811:817	the highly glycosylated protein	787:817	the highly glycosylated protein	787:817	We describe a simple, relatively inexpensive procedure in which standard commercial media is supplemented with 13C-enriched glucose to achieve labeling of all glycans plus all alanines of the N-terminal domain of the highly glycosylated protein, CEACAM1.
32902634	4	3	gly	glycosylated	798:809	arg1	CEACAM1					820:826	CEACAM1	820:826	CEACAM1	820:826	We describe a simple, relatively inexpensive procedure in which standard commercial media is supplemented with 13C-enriched glucose to achieve labeling of all glycans plus all alanines of the N-terminal domain of the highly glycosylated protein, CEACAM1.
32902634	6	4	from	protein	1044:1050	arg1	culture					1099:1105	a single culture	1090:1105	a single culture requiring no additional technical expertise	1090:1149	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	5	5	theme	N-glycan	884:891	arg1	sites					893:897	partially occupied N-glycan sites	865:897	partially occupied N-glycan sites	865:897	We demonstrate an ability to detect partially occupied N-glycan sites, sites less susceptible to processing by an endoglycosidase, and some unexpected truncation of the amino acid sequence.
32902634	5	5	theme	N-glycan	884:891	arg1	sequence					1009:1016	the amino acid sequence	994:1016	the amino acid sequence	994:1016	We demonstrate an ability to detect partially occupied N-glycan sites, sites less susceptible to processing by an endoglycosidase, and some unexpected truncation of the amino acid sequence.
32902634	4	6	theme	commercial	647:656	arg1	media					658:662	standard commercial media	638:662	standard commercial media	638:662	We describe a simple, relatively inexpensive procedure in which standard commercial media is supplemented with 13C-enriched glucose to achieve labeling of all glycans plus all alanines of the N-terminal domain of the highly glycosylated protein, CEACAM1.
32902634	6	7	theme	technical	1131:1139	arg1	expertise					1141:1149	no additional technical expertise	1117:1149	no additional technical expertise	1117:1149	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	2	8	used	used	412:415	arg2	substrates					394:403	low-cost isotopically enriched metabolic substrates	353:403	low-cost isotopically enriched metabolic substrates	353:403	This is routine for proteins that can be expressed in bacterial culture where low-cost isotopically enriched metabolic substrates can be used.
32902634	6	9	theme	functional	1269:1278	arg1	screening					1280:1288	structural and functional screening	1254:1288	structural and functional screening of the many glycosylated proteins important to human health	1254:1348	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	3	10	theme	enriched	535:542	arg1	acids					550:554	more costly isotopically enriched amino acids	510:554	more costly isotopically enriched amino acids	510:554	However, it can be expensive for glycosylated proteins expressed in mammalian culture where more costly isotopically enriched amino acids are usually used.
32902634	3	11	theme	glycosylated	451:462	arg1	proteins					464:471	glycosylated proteins	451:471	glycosylated proteins expressed in mammalian culture where more costly isotopically enriched amino acids are usually used	451:571	However, it can be expensive for glycosylated proteins expressed in mammalian culture where more costly isotopically enriched amino acids are usually used.
32902634	4	12	theme	N-terminal	766:775	arg1	protein					811:817	the highly glycosylated protein	787:817	the highly glycosylated protein	787:817	We describe a simple, relatively inexpensive procedure in which standard commercial media is supplemented with 13C-enriched glucose to achieve labeling of all glycans plus all alanines of the N-terminal domain of the highly glycosylated protein, CEACAM1.
32902634	4	12	theme	N-terminal	766:775	arg1	domain					777:782	the N-terminal domain	762:782	the N-terminal domain of the highly glycosylated protein, CEACAM1	762:826	We describe a simple, relatively inexpensive procedure in which standard commercial media is supplemented with 13C-enriched glucose to achieve labeling of all glycans plus all alanines of the N-terminal domain of the highly glycosylated protein, CEACAM1.
32902634	2	13	theme	enriched	375:382	arg1	substrates					394:403	low-cost isotopically enriched metabolic substrates	353:403	low-cost isotopically enriched metabolic substrates	353:403	This is routine for proteins that can be expressed in bacterial culture where low-cost isotopically enriched metabolic substrates can be used.
32902634	6	14	theme	structural	1254:1263	arg1	screening					1280:1288	structural and functional screening	1254:1288	structural and functional screening of the many glycosylated proteins important to human health	1254:1348	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	0	15	gly	glycoproteins	64:76	arg1	glycoproteins					64:76	glycoproteins	64:76	glycoproteins using 13C-glucose	64:94	Sparse isotope labeling for nuclear magnetic resonance (NMR) of glycoproteins using 13C-glucose.
32902634	4	16	theme	standard	638:645	arg1	media					658:662	standard commercial media	638:662	standard commercial media	638:662	We describe a simple, relatively inexpensive procedure in which standard commercial media is supplemented with 13C-enriched glucose to achieve labeling of all glycans plus all alanines of the N-terminal domain of the highly glycosylated protein, CEACAM1.
32902634	0	17	theme	isotope	7:13	arg1	labeling					15:22	isotope labeling	7:22	isotope labeling	7:22	Sparse isotope labeling for nuclear magnetic resonance (NMR) of glycoproteins using 13C-glucose.
32902634	6	18	from	labeling	1023:1030	arg1	culture					1099:1105	a single culture	1090:1105	a single culture requiring no additional technical expertise	1090:1149	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	4	19	theme	glycans	733:739	arg1	labeling					717:724	labeling	717:724	labeling of all glycans	717:739	We describe a simple, relatively inexpensive procedure in which standard commercial media is supplemented with 13C-enriched glucose to achieve labeling of all glycans plus all alanines of the N-terminal domain of the highly glycosylated protein, CEACAM1.
32902634	2	20	theme	low-cost	353:360	arg1	substrates					394:403	low-cost isotopically enriched metabolic substrates	353:403	low-cost isotopically enriched metabolic substrates	353:403	This is routine for proteins that can be expressed in bacterial culture where low-cost isotopically enriched metabolic substrates can be used.
32902634	6	21	theme	additional	1120:1129	arg1	expertise					1141:1149	no additional technical expertise	1117:1149	no additional technical expertise	1117:1149	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	1	22	theme	larger	177:182	arg1	proteins					184:191	larger proteins	177:191	larger proteins	177:191	Preparation of samples for nuclear magnetic resonance (NMR) characterization of larger proteins requires enrichment with less abundant, NMR-active, isotopes such as 13C and 15N.
32902634	6	23	contain	have	1217:1220	arg2	applications					1230:1241	several applications	1222:1241	several applications	1222:1241	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	6	23	contain	have	1217:1220	arg1	labeling					1023:1030	The labeling	1019:1030	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements	1019:1197	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	6	23	contain	have	1217:1220	arg2	screening					1280:1288	structural and functional screening	1254:1288	structural and functional screening of the many glycosylated proteins important to human health	1254:1348	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	1	24	with	enrichment	202:211	arg1	15N					270:272	15N	270:272	15N	270:272	Preparation of samples for nuclear magnetic resonance (NMR) characterization of larger proteins requires enrichment with less abundant, NMR-active, isotopes such as 13C and 15N.
32902634	1	24	with	enrichment	202:211	arg1	isotopes					245:252	less abundant, NMR-active, isotopes	218:252	less abundant, NMR-active, isotopes such as 13C and 15N	218:272	Preparation of samples for nuclear magnetic resonance (NMR) characterization of larger proteins requires enrichment with less abundant, NMR-active, isotopes such as 13C and 15N.
32902634	1	24	with	enrichment	202:211	arg1	13C					262:264	13C	262:264	13C	262:264	Preparation of samples for nuclear magnetic resonance (NMR) characterization of larger proteins requires enrichment with less abundant, NMR-active, isotopes such as 13C and 15N.
32902634	3	25	theme	amino	544:548	arg1	acids					550:554	more costly isotopically enriched amino acids	510:554	more costly isotopically enriched amino acids	510:554	However, it can be expensive for glycosylated proteins expressed in mammalian culture where more costly isotopically enriched amino acids are usually used.
32902634	5	26	theme	acid	1004:1007	arg1	sequence					1009:1016	the amino acid sequence	994:1016	the amino acid sequence	994:1016	We demonstrate an ability to detect partially occupied N-glycan sites, sites less susceptible to processing by an endoglycosidase, and some unexpected truncation of the amino acid sequence.
32902634	1	27	theme	proteins	184:191	arg1	characterization					157:172	nuclear magnetic resonance (NMR) characterization	124:172	nuclear magnetic resonance (NMR) characterization of larger proteins	124:191	Preparation of samples for nuclear magnetic resonance (NMR) characterization of larger proteins requires enrichment with less abundant, NMR-active, isotopes such as 13C and 15N.
32902634	6	28	dep	protein	1044:1050	arg1	requirements					1186:1197	past standard mammalian expression requirements	1151:1197	past standard mammalian expression requirements	1151:1197	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	6	29	theme	past	1151:1154	arg1	requirements					1186:1197	past standard mammalian expression requirements	1151:1197	past standard mammalian expression requirements	1151:1197	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	6	30	theme	mammalian	1165:1173	arg1	requirements					1186:1197	past standard mammalian expression requirements	1151:1197	past standard mammalian expression requirements	1151:1197	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	4	31	theme	13C-enriched	685:696	arg1	glucose					698:704	13C-enriched glucose	685:704	13C-enriched glucose	685:704	We describe a simple, relatively inexpensive procedure in which standard commercial media is supplemented with 13C-enriched glucose to achieve labeling of all glycans plus all alanines of the N-terminal domain of the highly glycosylated protein, CEACAM1.
32902634	5	32	theme	occupied	875:882	arg1	sites					893:897	partially occupied N-glycan sites	865:897	partially occupied N-glycan sites	865:897	We demonstrate an ability to detect partially occupied N-glycan sites, sites less susceptible to processing by an endoglycosidase, and some unexpected truncation of the amino acid sequence.
32902634	5	32	theme	occupied	875:882	arg1	sequence					1009:1016	the amino acid sequence	994:1016	the amino acid sequence	994:1016	We demonstrate an ability to detect partially occupied N-glycan sites, sites less susceptible to processing by an endoglycosidase, and some unexpected truncation of the amino acid sequence.
32902634	0	33	theme	magnetic	36:43	arg1	NMR					56:58	NMR	56:58	NMR	56:58	Sparse isotope labeling for nuclear magnetic resonance (NMR) of glycoproteins using 13C-glucose.
32902634	0	33	theme	magnetic	36:43	arg1	resonance					45:53	nuclear magnetic resonance	28:53	nuclear magnetic resonance (NMR) of glycoproteins using 13C-glucose	28:94	Sparse isotope labeling for nuclear magnetic resonance (NMR) of glycoproteins using 13C-glucose.
32902634	3	34	used	used	568:571	arg2	acids					550:554	more costly isotopically enriched amino acids	510:554	more costly isotopically enriched amino acids	510:554	However, it can be expensive for glycosylated proteins expressed in mammalian culture where more costly isotopically enriched amino acids are usually used.
32902634	6	35	theme	human	1337:1341	arg1	health					1343:1348	human health	1337:1348	human health	1337:1348	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	6	36	theme	single	1092:1097	arg1	culture					1099:1105	a single culture	1090:1105	a single culture requiring no additional technical expertise	1090:1149	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	4	37	theme	protein	811:817	arg1	protein					811:817	the highly glycosylated protein	787:817	the highly glycosylated protein	787:817	We describe a simple, relatively inexpensive procedure in which standard commercial media is supplemented with 13C-enriched glucose to achieve labeling of all glycans plus all alanines of the N-terminal domain of the highly glycosylated protein, CEACAM1.
32902634	4	37	theme	protein	811:817	arg1	domain					777:782	the N-terminal domain	762:782	the N-terminal domain of the highly glycosylated protein, CEACAM1	762:826	We describe a simple, relatively inexpensive procedure in which standard commercial media is supplemented with 13C-enriched glucose to achieve labeling of all glycans plus all alanines of the N-terminal domain of the highly glycosylated protein, CEACAM1.
32902634	6	38	gly	glycosylated	1302:1313	arg1	proteins					1315:1322	the many glycosylated proteins	1293:1322	the many glycosylated proteins important to human health	1293:1348	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	0	39	theme	nuclear	28:34	arg1	NMR					56:58	NMR	56:58	NMR	56:58	Sparse isotope labeling for nuclear magnetic resonance (NMR) of glycoproteins using 13C-glucose.
32902634	0	39	theme	nuclear	28:34	arg1	resonance					45:53	nuclear magnetic resonance	28:53	nuclear magnetic resonance (NMR) of glycoproteins using 13C-glucose	28:94	Sparse isotope labeling for nuclear magnetic resonance (NMR) of glycoproteins using 13C-glucose.
32902634	6	40	theme	standard	1156:1163	arg1	requirements					1186:1197	past standard mammalian expression requirements	1151:1197	past standard mammalian expression requirements	1151:1197	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	4	41	theme	glycosylated	798:809	arg1	protein					811:817	the highly glycosylated protein	787:817	the highly glycosylated protein	787:817	We describe a simple, relatively inexpensive procedure in which standard commercial media is supplemented with 13C-enriched glucose to achieve labeling of all glycans plus all alanines of the N-terminal domain of the highly glycosylated protein, CEACAM1.
32902634	4	41	theme	glycosylated	798:809	arg1	CEACAM1					820:826	CEACAM1	820:826	CEACAM1	820:826	We describe a simple, relatively inexpensive procedure in which standard commercial media is supplemented with 13C-enriched glucose to achieve labeling of all glycans plus all alanines of the N-terminal domain of the highly glycosylated protein, CEACAM1.
32902634	2	42	theme	metabolic	384:392	arg1	substrates					394:403	low-cost isotopically enriched metabolic substrates	353:403	low-cost isotopically enriched metabolic substrates	353:403	This is routine for proteins that can be expressed in bacterial culture where low-cost isotopically enriched metabolic substrates can be used.
32902634	6	43	theme	important	1324:1332	arg1	proteins					1315:1322	the many glycosylated proteins	1293:1322	the many glycosylated proteins important to human health	1293:1348	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	1	44	dep	abundant	223:230	arg1	NMR-active					233:242	NMR-active	233:242	NMR-active	233:242	Preparation of samples for nuclear magnetic resonance (NMR) characterization of larger proteins requires enrichment with less abundant, NMR-active, isotopes such as 13C and 15N.
32902634	6	45	theme	glycans	1079:1085	arg1	labeling					1023:1030	The labeling	1019:1030	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements	1019:1197	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	6	46	theme	protein	1044:1050	arg1	labeling					1023:1030	The labeling	1019:1030	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements	1019:1197	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	6	47	theme	several	1222:1228	arg1	screening					1280:1288	structural and functional screening	1254:1288	structural and functional screening of the many glycosylated proteins important to human health	1254:1348	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	6	47	theme	several	1222:1228	arg1	applications					1230:1241	several applications	1222:1241	several applications	1222:1241	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	6	48	theme	proteins	1315:1322	arg1	screening					1280:1288	structural and functional screening	1254:1288	structural and functional screening of the many glycosylated proteins important to human health	1254:1348	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	1	49	theme	resonance	141:149	arg1	characterization					157:172	nuclear magnetic resonance (NMR) characterization	124:172	nuclear magnetic resonance (NMR) characterization of larger proteins	124:191	Preparation of samples for nuclear magnetic resonance (NMR) characterization of larger proteins requires enrichment with less abundant, NMR-active, isotopes such as 13C and 15N.
32902634	6	50	from	culture	1099:1105	arg1	labeling					1023:1030	The labeling	1019:1030	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements	1019:1197	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	1	51	theme	samples	112:118	arg1	Preparation					97:107	Preparation	97:107	Preparation of samples for nuclear magnetic resonance (NMR) characterization of larger proteins	97:191	Preparation of samples for nuclear magnetic resonance (NMR) characterization of larger proteins requires enrichment with less abundant, NMR-active, isotopes such as 13C and 15N.
32902634	6	52	theme	expression	1175:1184	arg1	requirements					1186:1197	past standard mammalian expression requirements	1151:1197	past standard mammalian expression requirements	1151:1197	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	6	53	theme	glycosylated	1302:1313	arg1	proteins					1315:1322	the many glycosylated proteins	1293:1322	the many glycosylated proteins important to human health	1293:1348	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	5	54	theme	sequence	1009:1016	arg1	sites					893:897	partially occupied N-glycan sites	865:897	partially occupied N-glycan sites	865:897	We demonstrate an ability to detect partially occupied N-glycan sites, sites less susceptible to processing by an endoglycosidase, and some unexpected truncation of the amino acid sequence.
32902634	5	54	theme	sequence	1009:1016	arg1	sequence					1009:1016	the amino acid sequence	994:1016	the amino acid sequence	994:1016	We demonstrate an ability to detect partially occupied N-glycan sites, sites less susceptible to processing by an endoglycosidase, and some unexpected truncation of the amino acid sequence.
32902634	5	54	theme	sequence	1009:1016	arg1	truncation					980:989	some unexpected truncation	964:989	some unexpected truncation of the amino acid sequence	964:1016	We demonstrate an ability to detect partially occupied N-glycan sites, sites less susceptible to processing by an endoglycosidase, and some unexpected truncation of the amino acid sequence.
32902634	5	54	theme	sequence	1009:1016	arg1	sites					900:904	sites	900:904	sites less susceptible to processing by an endoglycosidase	900:957	We demonstrate an ability to detect partially occupied N-glycan sites, sites less susceptible to processing by an endoglycosidase, and some unexpected truncation of the amino acid sequence.
32902634	5	55	theme	susceptible	911:921	arg1	sequence					1009:1016	the amino acid sequence	994:1016	the amino acid sequence	994:1016	We demonstrate an ability to detect partially occupied N-glycan sites, sites less susceptible to processing by an endoglycosidase, and some unexpected truncation of the amino acid sequence.
32902634	5	55	theme	susceptible	911:921	arg1	sites					900:904	sites	900:904	sites less susceptible to processing by an endoglycosidase	900:957	We demonstrate an ability to detect partially occupied N-glycan sites, sites less susceptible to processing by an endoglycosidase, and some unexpected truncation of the amino acid sequence.
32902634	3	56	gly	glycosylated	451:462	arg1	proteins					464:471	glycosylated proteins	451:471	glycosylated proteins expressed in mammalian culture where more costly isotopically enriched amino acids are usually used	451:571	However, it can be expensive for glycosylated proteins expressed in mammalian culture where more costly isotopically enriched amino acids are usually used.
32902634	4	57	theme	simple	588:593	arg1	procedure					619:627	a simple, relatively inexpensive procedure	586:627	a simple, relatively inexpensive procedure in which standard commercial media is supplemented with 13C-enriched glucose to achieve labeling of all glycans plus all alanines of the N-terminal domain of the highly glycosylated protein, CEACAM1	586:826	We describe a simple, relatively inexpensive procedure in which standard commercial media is supplemented with 13C-enriched glucose to achieve labeling of all glycans plus all alanines of the N-terminal domain of the highly glycosylated protein, CEACAM1.
32902634	5	58	theme	unexpected	969:978	arg1	truncation					980:989	some unexpected truncation	964:989	some unexpected truncation of the amino acid sequence	964:1016	We demonstrate an ability to detect partially occupied N-glycan sites, sites less susceptible to processing by an endoglycosidase, and some unexpected truncation of the amino acid sequence.
32902634	4	59	dep	simple	588:593	arg1	inexpensive					607:617	inexpensive	607:617	inexpensive	607:617	We describe a simple, relatively inexpensive procedure in which standard commercial media is supplemented with 13C-enriched glucose to achieve labeling of all glycans plus all alanines of the N-terminal domain of the highly glycosylated protein, CEACAM1.
32902634	4	60	theme	domain	777:782	arg1	alanines					750:757	all alanines	746:757	all alanines of the N-terminal domain of the highly glycosylated protein, CEACAM1	746:826	We describe a simple, relatively inexpensive procedure in which standard commercial media is supplemented with 13C-enriched glucose to achieve labeling of all glycans plus all alanines of the N-terminal domain of the highly glycosylated protein, CEACAM1.
32902634	1	61	theme	nuclear	124:130	arg1	resonance					141:149	nuclear magnetic resonance	124:149	nuclear magnetic resonance (NMR) characterization of larger proteins	124:191	Preparation of samples for nuclear magnetic resonance (NMR) characterization of larger proteins requires enrichment with less abundant, NMR-active, isotopes such as 13C and 15N.
32902634	1	61	theme	nuclear	124:130	arg1	NMR					152:154	NMR	152:154	NMR	152:154	Preparation of samples for nuclear magnetic resonance (NMR) characterization of larger proteins requires enrichment with less abundant, NMR-active, isotopes such as 13C and 15N.
32902634	1	62	theme	abundant	223:230	arg1	15N					270:272	15N	270:272	15N	270:272	Preparation of samples for nuclear magnetic resonance (NMR) characterization of larger proteins requires enrichment with less abundant, NMR-active, isotopes such as 13C and 15N.
32902634	1	62	theme	abundant	223:230	arg1	isotopes					245:252	less abundant, NMR-active, isotopes	218:252	less abundant, NMR-active, isotopes such as 13C and 15N	218:272	Preparation of samples for nuclear magnetic resonance (NMR) characterization of larger proteins requires enrichment with less abundant, NMR-active, isotopes such as 13C and 15N.
32902634	1	62	theme	abundant	223:230	arg1	13C					262:264	13C	262:264	13C	262:264	Preparation of samples for nuclear magnetic resonance (NMR) characterization of larger proteins requires enrichment with less abundant, NMR-active, isotopes such as 13C and 15N.
32902634	1	63	theme	magnetic	132:139	arg1	resonance					141:149	nuclear magnetic resonance	124:149	nuclear magnetic resonance (NMR) characterization of larger proteins	124:191	Preparation of samples for nuclear magnetic resonance (NMR) characterization of larger proteins requires enrichment with less abundant, NMR-active, isotopes such as 13C and 15N.
32902634	1	63	theme	magnetic	132:139	arg1	NMR					152:154	NMR	152:154	NMR	152:154	Preparation of samples for nuclear magnetic resonance (NMR) characterization of larger proteins requires enrichment with less abundant, NMR-active, isotopes such as 13C and 15N.
32902634	5	64	theme	amino	998:1002	arg1	sequence					1009:1016	the amino acid sequence	994:1016	the amino acid sequence	994:1016	We demonstrate an ability to detect partially occupied N-glycan sites, sites less susceptible to processing by an endoglycosidase, and some unexpected truncation of the amino acid sequence.
32902634	6	65	from	glycans	1079:1085	arg1	culture					1099:1105	a single culture	1090:1105	a single culture requiring no additional technical expertise	1090:1149	The labeling of both the protein (through alanines) and the glycans in a single culture requiring no additional technical expertise past standard mammalian expression requirements is anticipated to have several applications, including structural and functional screening of the many glycosylated proteins important to human health.
32902634	2	66	theme	bacterial	329:337	arg1	culture					339:345	bacterial culture	329:345	bacterial culture where low-cost isotopically enriched metabolic substrates can be used	329:415	This is routine for proteins that can be expressed in bacterial culture where low-cost isotopically enriched metabolic substrates can be used.
34126144	0	0	theme	nano	95:98	arg1	particles					100:108	ZnO nano particles	91:108	ZnO nano particles for accelerating dermal burn healing	91:145	Studies on novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing: In vivo and in vitro evaluation.
34126144	4	1	theme	tested	945:950	arg1	bacteria					952:959	the tested bacteria	941:959	the tested bacteria	941:959	The antibacterial activity of the films was investigated against Staphylococcus aureus and Pseudomonas aeruginosa bacteria and it presents good inhibitory activities against the tested bacteria as compared to the control sample.
34126144	7	2	theme	wound	1398:1402	arg1	border					1404:1409	the wound border	1394:1409	the wound border	1394:1409	In the control sample (gauze), no re-epithelialized area was observed, except in close proximity of the wound border.
34126144	8	3	theme	full	1445:1448	arg1	coverage					1450:1457	its full coverage	1441:1457	its full coverage of the wounds with new epithelium and hair follicles	1441:1510	The results show that due to its full coverage of the wounds with new epithelium and hair follicles, bentonite-containing composites are more preferred.
34126144	0	4	theme	ZnO	91:93	arg1	particles					100:108	ZnO nano particles	91:108	ZnO nano particles for accelerating dermal burn healing	91:145	Studies on novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing: In vivo and in vitro evaluation.
34126144	7	5	located	observed	1355:1362	arg1	sample					1309:1314	the control sample	1297:1314	the control sample (gauze)	1297:1322	In the control sample (gauze), no re-epithelialized area was observed, except in close proximity of the wound border.
34126144	7	5	located	observed	1355:1362	arg2	area					1346:1349	no re-epithelialized area	1325:1349	no re-epithelialized area	1325:1349	In the control sample (gauze), no re-epithelialized area was observed, except in close proximity of the wound border.
34126144	7	5	located	observed	1355:1362	arg1	gauze					1317:1321	gauze	1317:1321	gauze	1317:1321	In the control sample (gauze), no re-epithelialized area was observed, except in close proximity of the wound border.
34126144	4	6	theme	inhibitory	911:920	arg1	activities					922:931	good inhibitory activities	906:931	good inhibitory activities	906:931	The antibacterial activity of the films was investigated against Staphylococcus aureus and Pseudomonas aeruginosa bacteria and it presents good inhibitory activities against the tested bacteria as compared to the control sample.
34126144	3	7	theme	composite	695:703	arg1	films					705:709	the chitosan/bentonite composite films	672:709	the chitosan/bentonite composite films	672:709	Bentonite containing films illustrate more flexibility than alginate ones while the chitosan/bentonite composite films have a maximum water absorption capacity of about 170%.
34126144	8	8	with	coverage	1450:1457	arg1	follicles					1502:1510	hair follicles	1497:1510	hair follicles	1497:1510	The results show that due to its full coverage of the wounds with new epithelium and hair follicles, bentonite-containing composites are more preferred.
34126144	8	8	with	coverage	1450:1457	arg1	epithelium					1482:1491	new epithelium	1478:1491	new epithelium	1478:1491	The results show that due to its full coverage of the wounds with new epithelium and hair follicles, bentonite-containing composites are more preferred.
34126144	0	9	from	Studies	0:6	arg1	films					67:71	novel chitosan/alginate and chitosan/bentonite flexible films	11:71	novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing	11:145	Studies on novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing: In vivo and in vitro evaluation.
34126144	1	10	theme	different	291:299	arg1	ratios					306:311	different mass ratios	291:311	different mass ratios incorporated with nano particles of Zinc Oxide, which were achieved through the method of green synthesis from Nettle leaf extract	291:442	This research work was performed to prepare chitosan-alginate-gelatin and chitosan-bentonite-gelatin films in different mass ratios incorporated with nano particles of Zinc Oxide, which were achieved through the method of green synthesis from Nettle leaf extract.
34126144	5	11	theme	animal	1014:1019	arg1	tests					1021:1025	vivo animal tests	1009:1025	vivo animal tests	1009:1025	Furthermore, vivo animal tests were performed to confirm the applicability of the prepared films as a healing material for burned skin.
34126144	6	12	theme	normal	1224:1229	arg1	structures					1231:1240	normal structures	1224:1240	normal structures	1224:1240	Skin appendages, such as hair follicles and sebaceous gland in the dermis, were detected in normal structures by applying both of the composites to damaged skin.
34126144	1	13	theme	mass	301:304	arg1	ratios					306:311	different mass ratios	291:311	different mass ratios incorporated with nano particles of Zinc Oxide, which were achieved through the method of green synthesis from Nettle leaf extract	291:442	This research work was performed to prepare chitosan-alginate-gelatin and chitosan-bentonite-gelatin films in different mass ratios incorporated with nano particles of Zinc Oxide, which were achieved through the method of green synthesis from Nettle leaf extract.
34126144	0	14	theme	dermal	127:132	arg1	healing					139:145	dermal burn healing	127:145	dermal burn healing	127:145	Studies on novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing: In vivo and in vitro evaluation.
34126144	8	15	theme	bentonite-containing	1513:1532	arg1	composites					1534:1543	bentonite-containing composites	1513:1543	bentonite-containing composites	1513:1543	The results show that due to its full coverage of the wounds with new epithelium and hair follicles, bentonite-containing composites are more preferred.
34126144	2	16	theme	surface	572:578	arg1	morphology					580:589	surface morphology	572:589	surface morphology	572:589	The films were prepared and characterized based on their physicochemical properties, such as water absorption and porosity and surface morphology.
34126144	6	17	theme	Skin	1132:1135	arg1	gland					1186:1190	sebaceous gland	1176:1190	sebaceous gland	1176:1190	Skin appendages, such as hair follicles and sebaceous gland in the dermis, were detected in normal structures by applying both of the composites to damaged skin.
34126144	6	17	theme	Skin	1132:1135	arg1	follicles					1162:1170	hair follicles	1157:1170	hair follicles	1157:1170	Skin appendages, such as hair follicles and sebaceous gland in the dermis, were detected in normal structures by applying both of the composites to damaged skin.
34126144	6	17	theme	Skin	1132:1135	arg1	appendages					1137:1146	Skin appendages	1132:1146	Skin appendages	1132:1146	Skin appendages, such as hair follicles and sebaceous gland in the dermis, were detected in normal structures by applying both of the composites to damaged skin.
34126144	2	18	theme	physicochemical	502:516	arg1	porosity					559:566	porosity	559:566	porosity	559:566	The films were prepared and characterized based on their physicochemical properties, such as water absorption and porosity and surface morphology.
34126144	2	18	theme	physicochemical	502:516	arg1	absorption					544:553	water absorption	538:553	water absorption	538:553	The films were prepared and characterized based on their physicochemical properties, such as water absorption and porosity and surface morphology.
34126144	2	18	theme	physicochemical	502:516	arg1	properties					518:527	their physicochemical properties	496:527	their physicochemical properties	496:527	The films were prepared and characterized based on their physicochemical properties, such as water absorption and porosity and surface morphology.
34126144	4	19	theme	good	906:909	arg1	activities					922:931	good inhibitory activities	906:931	good inhibitory activities	906:931	The antibacterial activity of the films was investigated against Staphylococcus aureus and Pseudomonas aeruginosa bacteria and it presents good inhibitory activities against the tested bacteria as compared to the control sample.
34126144	5	20	theme	vivo	1009:1012	arg1	tests					1021:1025	vivo animal tests	1009:1025	vivo animal tests	1009:1025	Furthermore, vivo animal tests were performed to confirm the applicability of the prepared films as a healing material for burned skin.
34126144	2	21	theme	water	538:542	arg1	absorption					544:553	water absorption	538:553	water absorption	538:553	The films were prepared and characterized based on their physicochemical properties, such as water absorption and porosity and surface morphology.
34126144	1	22	from	method	393:398	arg1	extract					436:442	Nettle leaf extract	424:442	Nettle leaf extract	424:442	This research work was performed to prepare chitosan-alginate-gelatin and chitosan-bentonite-gelatin films in different mass ratios incorporated with nano particles of Zinc Oxide, which were achieved through the method of green synthesis from Nettle leaf extract.
34126144	1	23	from	extract	436:442	arg1	method					393:398	the method	389:398	the method of green synthesis from Nettle leaf extract	389:442	This research work was performed to prepare chitosan-alginate-gelatin and chitosan-bentonite-gelatin films in different mass ratios incorporated with nano particles of Zinc Oxide, which were achieved through the method of green synthesis from Nettle leaf extract.
34126144	1	23	from	extract	436:442	arg1	synthesis					409:417	green synthesis	403:417	green synthesis from Nettle leaf extract	403:442	This research work was performed to prepare chitosan-alginate-gelatin and chitosan-bentonite-gelatin films in different mass ratios incorporated with nano particles of Zinc Oxide, which were achieved through the method of green synthesis from Nettle leaf extract.
34126144	3	24	theme	containing	602:611	arg1	films					613:617	Bentonite containing films	592:617	Bentonite containing films	592:617	Bentonite containing films illustrate more flexibility than alginate ones while the chitosan/bentonite composite films have a maximum water absorption capacity of about 170%.
34126144	3	25	dep	flexibility	635:645	arg1	have					711:714	have	711:714	have a maximum water absorption capacity of about 170%	711:764	Bentonite containing films illustrate more flexibility than alginate ones while the chitosan/bentonite composite films have a maximum water absorption capacity of about 170%.
34126144	8	26	theme	new	1478:1480	arg1	epithelium					1482:1491	new epithelium	1478:1491	new epithelium	1478:1491	The results show that due to its full coverage of the wounds with new epithelium and hair follicles, bentonite-containing composites are more preferred.
34126144	3	27	theme	water	726:730	arg1	capacity					743:750	a maximum water absorption capacity	716:750	a maximum water absorption capacity of about 170%	716:764	Bentonite containing films illustrate more flexibility than alginate ones while the chitosan/bentonite composite films have a maximum water absorption capacity of about 170%.
34126144	1	28	theme	research	186:193	arg1	work					195:198	This research work	181:198	This research work	181:198	This research work was performed to prepare chitosan-alginate-gelatin and chitosan-bentonite-gelatin films in different mass ratios incorporated with nano particles of Zinc Oxide, which were achieved through the method of green synthesis from Nettle leaf extract.
34126144	0	29	theme	chitosan/alginate	17:33	arg1	films					67:71	novel chitosan/alginate and chitosan/bentonite flexible films	11:71	novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing	11:145	Studies on novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing: In vivo and in vitro evaluation.
34126144	5	30	theme	healing	1098:1104	arg1	material					1106:1113	a healing material	1096:1113	a healing material for burned skin	1096:1129	Furthermore, vivo animal tests were performed to confirm the applicability of the prepared films as a healing material for burned skin.
34126144	5	30	theme	healing	1098:1104	arg1	applicability					1057:1069	the applicability	1053:1069	the applicability of the prepared films	1053:1091	Furthermore, vivo animal tests were performed to confirm the applicability of the prepared films as a healing material for burned skin.
34126144	4	31	theme	films	801:805	arg1	activity					785:792	The antibacterial activity	767:792	The antibacterial activity of the films	767:805	The antibacterial activity of the films was investigated against Staphylococcus aureus and Pseudomonas aeruginosa bacteria and it presents good inhibitory activities against the tested bacteria as compared to the control sample.
34126144	6	32	theme	damaged	1280:1286	arg1	skin					1288:1291	damaged skin	1280:1291	damaged skin	1280:1291	Skin appendages, such as hair follicles and sebaceous gland in the dermis, were detected in normal structures by applying both of the composites to damaged skin.
34126144	0	33	theme	novel	11:15	arg1	films					67:71	novel chitosan/alginate and chitosan/bentonite flexible films	11:71	novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing	11:145	Studies on novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing: In vivo and in vitro evaluation.
34126144	0	34	theme	burn	134:137	arg1	healing					139:145	dermal burn healing	127:145	dermal burn healing	127:145	Studies on novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing: In vivo and in vitro evaluation.
34126144	1	35	theme	green	403:407	arg1	synthesis					409:417	green synthesis	403:417	green synthesis from Nettle leaf extract	403:442	This research work was performed to prepare chitosan-alginate-gelatin and chitosan-bentonite-gelatin films in different mass ratios incorporated with nano particles of Zinc Oxide, which were achieved through the method of green synthesis from Nettle leaf extract.
34126144	3	36	theme	Bentonite	592:600	arg1	films					613:617	Bentonite containing films	592:617	Bentonite containing films	592:617	Bentonite containing films illustrate more flexibility than alginate ones while the chitosan/bentonite composite films have a maximum water absorption capacity of about 170%.
34126144	7	37	theme	re-epithelialized	1328:1344	arg1	area					1346:1349	no re-epithelialized area	1325:1349	no re-epithelialized area	1325:1349	In the control sample (gauze), no re-epithelialized area was observed, except in close proximity of the wound border.
34126144	0	38	theme	chitosan/bentonite	39:56	arg1	films					67:71	novel chitosan/alginate and chitosan/bentonite flexible films	11:71	novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing	11:145	Studies on novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing: In vivo and in vitro evaluation.
34126144	1	39	theme	nano	331:334	arg1	particles					336:344	nano particles	331:344	nano particles of Zinc Oxide, which were achieved through the method of green synthesis from Nettle leaf extract	331:442	This research work was performed to prepare chitosan-alginate-gelatin and chitosan-bentonite-gelatin films in different mass ratios incorporated with nano particles of Zinc Oxide, which were achieved through the method of green synthesis from Nettle leaf extract.
34126144	0	40	theme	In	148:149	arg1	evaluation					169:178	In vivo and in vitro evaluation	148:178	In vivo and in vitro evaluation	148:178	Studies on novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing: In vivo and in vitro evaluation.
34126144	1	41	theme	synthesis	409:417	arg1	method					393:398	the method	389:398	the method of green synthesis from Nettle leaf extract	389:442	This research work was performed to prepare chitosan-alginate-gelatin and chitosan-bentonite-gelatin films in different mass ratios incorporated with nano particles of Zinc Oxide, which were achieved through the method of green synthesis from Nettle leaf extract.
34126144	5	42	theme	films	1087:1091	arg1	applicability					1057:1069	the applicability	1053:1069	the applicability of the prepared films	1053:1091	Furthermore, vivo animal tests were performed to confirm the applicability of the prepared films as a healing material for burned skin.
34126144	5	42	theme	films	1087:1091	arg1	material					1106:1113	a healing material	1096:1113	a healing material for burned skin	1096:1129	Furthermore, vivo animal tests were performed to confirm the applicability of the prepared films as a healing material for burned skin.
34126144	4	43	dep	aureus	847:852	arg1	bacteria					881:888	bacteria	881:888	bacteria	881:888	The antibacterial activity of the films was investigated against Staphylococcus aureus and Pseudomonas aeruginosa bacteria and it presents good inhibitory activities against the tested bacteria as compared to the control sample.
34126144	3	44	contain	have	711:714	arg1	films					705:709	the chitosan/bentonite composite films	672:709	the chitosan/bentonite composite films	672:709	Bentonite containing films illustrate more flexibility than alginate ones while the chitosan/bentonite composite films have a maximum water absorption capacity of about 170%.
34126144	3	44	contain	have	711:714	arg2	capacity					743:750	a maximum water absorption capacity	716:750	a maximum water absorption capacity of about 170%	716:764	Bentonite containing films illustrate more flexibility than alginate ones while the chitosan/bentonite composite films have a maximum water absorption capacity of about 170%.
34126144	6	45	theme	sebaceous	1176:1184	arg1	gland					1186:1190	sebaceous gland	1176:1190	sebaceous gland	1176:1190	Skin appendages, such as hair follicles and sebaceous gland in the dermis, were detected in normal structures by applying both of the composites to damaged skin.
34126144	3	46	theme	chitosan/bentonite	676:693	arg1	films					705:709	the chitosan/bentonite composite films	672:709	the chitosan/bentonite composite films	672:709	Bentonite containing films illustrate more flexibility than alginate ones while the chitosan/bentonite composite films have a maximum water absorption capacity of about 170%.
34126144	7	47	theme	border	1404:1409	arg1	proximity					1381:1389	close proximity	1375:1389	close proximity of the wound border	1375:1409	In the control sample (gauze), no re-epithelialized area was observed, except in close proximity of the wound border.
34126144	0	48	dep	evaluation	169:178	arg1	Studies					0:6	Studies	0:6	Studies on novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing	0:145	Studies on novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing: In vivo and in vitro evaluation.
34126144	1	49	theme	Nettle	424:429	arg1	extract					436:442	Nettle leaf extract	424:442	Nettle leaf extract	424:442	This research work was performed to prepare chitosan-alginate-gelatin and chitosan-bentonite-gelatin films in different mass ratios incorporated with nano particles of Zinc Oxide, which were achieved through the method of green synthesis from Nettle leaf extract.
34126144	3	50	theme	%	764:764	arg1	capacity					743:750	a maximum water absorption capacity	716:750	a maximum water absorption capacity of about 170%	716:764	Bentonite containing films illustrate more flexibility than alginate ones while the chitosan/bentonite composite films have a maximum water absorption capacity of about 170%.
34126144	0	51	theme	flexible	58:65	arg1	films					67:71	novel chitosan/alginate and chitosan/bentonite flexible films	11:71	novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing	11:145	Studies on novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing: In vivo and in vitro evaluation.
34126144	1	52	theme	Zinc	349:352	arg1	Oxide					354:358	Zinc Oxide	349:358	Zinc Oxide	349:358	This research work was performed to prepare chitosan-alginate-gelatin and chitosan-bentonite-gelatin films in different mass ratios incorporated with nano particles of Zinc Oxide, which were achieved through the method of green synthesis from Nettle leaf extract.
34126144	7	53	theme	control	1301:1307	arg1	sample					1309:1314	the control sample	1297:1314	the control sample (gauze)	1297:1322	In the control sample (gauze), no re-epithelialized area was observed, except in close proximity of the wound border.
34126144	7	53	theme	control	1301:1307	arg1	gauze					1317:1321	gauze	1317:1321	gauze	1317:1321	In the control sample (gauze), no re-epithelialized area was observed, except in close proximity of the wound border.
34126144	1	54	theme	leaf	431:434	arg1	extract					436:442	Nettle leaf extract	424:442	Nettle leaf extract	424:442	This research work was performed to prepare chitosan-alginate-gelatin and chitosan-bentonite-gelatin films in different mass ratios incorporated with nano particles of Zinc Oxide, which were achieved through the method of green synthesis from Nettle leaf extract.
34126144	3	55	theme	more	630:633	arg1	flexibility					635:645	more flexibility	630:645	more flexibility than alginate ones while the chitosan/bentonite composite films have a maximum water absorption capacity of about 170%	630:764	Bentonite containing films illustrate more flexibility than alginate ones while the chitosan/bentonite composite films have a maximum water absorption capacity of about 170%.
34126144	0	56	dep	in	160:161	arg1	vitro					163:167	vitro	163:167	vitro	163:167	Studies on novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing: In vivo and in vitro evaluation.
34126144	1	57	theme	Oxide	354:358	arg1	particles					336:344	nano particles	331:344	nano particles of Zinc Oxide, which were achieved through the method of green synthesis from Nettle leaf extract	331:442	This research work was performed to prepare chitosan-alginate-gelatin and chitosan-bentonite-gelatin films in different mass ratios incorporated with nano particles of Zinc Oxide, which were achieved through the method of green synthesis from Nettle leaf extract.
34126144	4	58	theme	control	980:986	arg1	sample					988:993	the control sample	976:993	the control sample	976:993	The antibacterial activity of the films was investigated against Staphylococcus aureus and Pseudomonas aeruginosa bacteria and it presents good inhibitory activities against the tested bacteria as compared to the control sample.
34126144	3	59	theme	absorption	732:741	arg1	capacity					743:750	a maximum water absorption capacity	716:750	a maximum water absorption capacity of about 170%	716:764	Bentonite containing films illustrate more flexibility than alginate ones while the chitosan/bentonite composite films have a maximum water absorption capacity of about 170%.
34126144	7	60	theme	close	1375:1379	arg1	proximity					1381:1389	close proximity	1375:1389	close proximity of the wound border	1375:1409	In the control sample (gauze), no re-epithelialized area was observed, except in close proximity of the wound border.
34126144	6	61	from	gland	1186:1190	arg1	dermis					1199:1204	the dermis	1195:1204	the dermis	1195:1204	Skin appendages, such as hair follicles and sebaceous gland in the dermis, were detected in normal structures by applying both of the composites to damaged skin.
34126144	4	62	theme	antibacterial	771:783	arg1	activity					785:792	The antibacterial activity	767:792	The antibacterial activity of the films	767:805	The antibacterial activity of the films was investigated against Staphylococcus aureus and Pseudomonas aeruginosa bacteria and it presents good inhibitory activities against the tested bacteria as compared to the control sample.
34126144	0	63	theme	in	160:161	arg1	evaluation					169:178	In vivo and in vitro evaluation	148:178	In vivo and in vitro evaluation	148:178	Studies on novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing: In vivo and in vitro evaluation.
34126144	5	64	theme	burned	1119:1124	arg1	skin					1126:1129	burned skin	1119:1129	burned skin	1119:1129	Furthermore, vivo animal tests were performed to confirm the applicability of the prepared films as a healing material for burned skin.
34126144	8	65	theme	wounds	1466:1471	arg1	coverage					1450:1457	its full coverage	1441:1457	its full coverage of the wounds with new epithelium and hair follicles	1441:1510	The results show that due to its full coverage of the wounds with new epithelium and hair follicles, bentonite-containing composites are more preferred.
34126144	1	66	theme	chitosan-alginate-gelatin	225:249	arg1	films					282:286	chitosan-alginate-gelatin and chitosan-bentonite-gelatin films	225:286	chitosan-alginate-gelatin and chitosan-bentonite-gelatin films	225:286	This research work was performed to prepare chitosan-alginate-gelatin and chitosan-bentonite-gelatin films in different mass ratios incorporated with nano particles of Zinc Oxide, which were achieved through the method of green synthesis from Nettle leaf extract.
34126144	3	67	theme	maximum	718:724	arg1	capacity					743:750	a maximum water absorption capacity	716:750	a maximum water absorption capacity of about 170%	716:764	Bentonite containing films illustrate more flexibility than alginate ones while the chitosan/bentonite composite films have a maximum water absorption capacity of about 170%.
34126144	3	68	theme	alginate	652:659	arg1	ones					661:664	alginate ones	652:664	alginate ones	652:664	Bentonite containing films illustrate more flexibility than alginate ones while the chitosan/bentonite composite films have a maximum water absorption capacity of about 170%.
34126144	0	69	dep	In	148:149	arg1	vivo					151:154	vivo	151:154	vivo	151:154	Studies on novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing: In vivo and in vitro evaluation.
34126144	6	70	located	detected	1212:1219	arg2	gland					1186:1190	sebaceous gland	1176:1190	sebaceous gland	1176:1190	Skin appendages, such as hair follicles and sebaceous gland in the dermis, were detected in normal structures by applying both of the composites to damaged skin.
34126144	6	70	located	detected	1212:1219	arg2	appendages					1137:1146	Skin appendages	1132:1146	Skin appendages	1132:1146	Skin appendages, such as hair follicles and sebaceous gland in the dermis, were detected in normal structures by applying both of the composites to damaged skin.
34126144	6	70	located	detected	1212:1219	arg2	follicles					1162:1170	hair follicles	1157:1170	hair follicles	1157:1170	Skin appendages, such as hair follicles and sebaceous gland in the dermis, were detected in normal structures by applying both of the composites to damaged skin.
34126144	6	70	located	detected	1212:1219	arg1	structures					1231:1240	normal structures	1224:1240	normal structures	1224:1240	Skin appendages, such as hair follicles and sebaceous gland in the dermis, were detected in normal structures by applying both of the composites to damaged skin.
34126144	8	71	theme	hair	1497:1500	arg1	follicles					1502:1510	hair follicles	1497:1510	hair follicles	1497:1510	The results show that due to its full coverage of the wounds with new epithelium and hair follicles, bentonite-containing composites are more preferred.
34126144	5	72	theme	prepared	1078:1085	arg1	films					1087:1091	the prepared films	1074:1091	the prepared films	1074:1091	Furthermore, vivo animal tests were performed to confirm the applicability of the prepared films as a healing material for burned skin.
34126144	6	73	from	follicles	1162:1170	arg1	dermis					1199:1204	the dermis	1195:1204	the dermis	1195:1204	Skin appendages, such as hair follicles and sebaceous gland in the dermis, were detected in normal structures by applying both of the composites to damaged skin.
34126144	6	74	theme	hair	1157:1160	arg1	follicles					1162:1170	hair follicles	1157:1170	hair follicles	1157:1170	Skin appendages, such as hair follicles and sebaceous gland in the dermis, were detected in normal structures by applying both of the composites to damaged skin.
34126144	1	75	theme	chitosan-bentonite-gelatin	255:280	arg1	films					282:286	chitosan-alginate-gelatin and chitosan-bentonite-gelatin films	225:286	chitosan-alginate-gelatin and chitosan-bentonite-gelatin films	225:286	This research work was performed to prepare chitosan-alginate-gelatin and chitosan-bentonite-gelatin films in different mass ratios incorporated with nano particles of Zinc Oxide, which were achieved through the method of green synthesis from Nettle leaf extract.
34702525	2	0	from	action	436:441	arg1	membranes					457:465	biological membranes	446:465	biological membranes	446:465	Despite the great photodynamic potential, erythrosine exhibits hydrophilicity, negatively impacting its action in biological membranes.
34702525	2	1	theme	photodynamic	350:361	arg1	potential					363:371	the great photodynamic potential	340:371	the great photodynamic potential	340:371	Despite the great photodynamic potential, erythrosine exhibits hydrophilicity, negatively impacting its action in biological membranes.
34702525	9	2	theme	drug	1424:1427	arg1	interaction					1405:1415	a higher interaction	1396:1415	a higher interaction of the drug with poly(ethylene oxide) (PEO)	1396:1459	In silico modelling indicated a higher interaction of the drug with poly(ethylene oxide) (PEO) than poly(propylene oxide) (PPO) fragments of polox407.
34702525	7	3	theme	sodium	1103:1108	arg1	carboxymethylcellulose					1110:1131	mucoadhesive sodium carboxymethylcellulose	1090:1131	mucoadhesive sodium carboxymethylcellulose	1090:1131	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC) were used as micellar systems for ERI or ERIs delivery.
34702525	9	4	theme	ethylene	1439:1446	arg1	oxide					1448:1452	ethylene oxide	1439:1452	ethylene oxide	1439:1452	In silico modelling indicated a higher interaction of the drug with poly(ethylene oxide) (PEO) than poly(propylene oxide) (PPO) fragments of polox407.
34702525	9	4	theme	ethylene	1439:1446	arg1	poly					1434:1437	poly	1434:1437	poly(ethylene oxide) (PEO)	1434:1459	In silico modelling indicated a higher interaction of the drug with poly(ethylene oxide) (PEO) than poly(propylene oxide) (PPO) fragments of polox407.
34702525	12	5	from	impact	1942:1947	arg1	preparations					1969:1980	HPMC preparations	1964:1980	HPMC preparations	1964:1980	In these mixtures, crystallinity, critical micellar temperature and enthalpy of polox407 micellisation were reduced, and micellar size, evaluated by transmission electron microscopy (TEM), showed low impact of ERI/ERIs in HPMC preparations.
34702525	5	6	theme	disodium	896:903	arg1	ERIs					911:914	ERIs	911:914	ERIs	911:914	In this work, mucoadhesive and thermoresponsive micellar systems were prepared containing erythrosine in two states: the native form (ERI) and the disodium salt (ERIs).
34702525	5	6	theme	disodium	896:903	arg1	states					858:863	two states	854:863	two states: the native form (ERI) and the disodium salt (ERIs)	854:915	In this work, mucoadhesive and thermoresponsive micellar systems were prepared containing erythrosine in two states: the native form (ERI) and the disodium salt (ERIs).
34702525	5	6	theme	disodium	896:903	arg1	salt					905:908	the disodium salt	892:908	the disodium salt (ERIs)	892:915	In this work, mucoadhesive and thermoresponsive micellar systems were prepared containing erythrosine in two states: the native form (ERI) and the disodium salt (ERIs).
34702525	12	7	theme	low	1938:1940	arg1	impact					1942:1947	low impact	1938:1947	low impact of ERI/ERIs in HPMC preparations	1938:1980	In these mixtures, crystallinity, critical micellar temperature and enthalpy of polox407 micellisation were reduced, and micellar size, evaluated by transmission electron microscopy (TEM), showed low impact of ERI/ERIs in HPMC preparations.
34702525	6	8	theme	ERI/ERIs	968:975	arg1	effect					958:963	the effect	954:963	the effect of ERI/ERIs on the micellar structure of the binary polymer mixtures	954:1032	The systems were evaluated based on the effect of ERI/ERIs on the micellar structure of the binary polymer mixtures.
34702525	4	9	theme	drug	743:746	arg1	retention					707:715	retention	707:715	retention of this topically applied drug	707:746	Moreover, using bioadhesive and thermoresponsive polymers to combine in situ gelation and bioadhesion may enhance retention of this topically applied drug.
34702525	13	10	theme	gels	2097:2100	arg1	formation					2084:2092	the micellar formation	2071:2092	the micellar formation of gels composed of 17.5% (w/w) polox407 and 3% (w/w) HPMC or 1% (w/w) NaCMC	2071:2169	Aiming toward photodynamic applications, the findings showed how ERI or ERIs can affect the micellar formation of gels composed of 17.5% (w/w) polox407 and 3% (w/w) HPMC or 1% (w/w) NaCMC, important for understating their behaviour and future utilisation as erythrosine delivery systems.
34702525	13	11	theme	micellar	2075:2082	arg1	formation					2084:2092	the micellar formation	2071:2092	the micellar formation of gels composed of 17.5% (w/w) polox407 and 3% (w/w) HPMC or 1% (w/w) NaCMC	2071:2169	Aiming toward photodynamic applications, the findings showed how ERI or ERIs can affect the micellar formation of gels composed of 17.5% (w/w) polox407 and 3% (w/w) HPMC or 1% (w/w) NaCMC, important for understating their behaviour and future utilisation as erythrosine delivery systems.
34702525	13	12	dep	%	2118:2118	arg1	NaCMC					2165:2169	NaCMC	2165:2169	NaCMC	2165:2169	Aiming toward photodynamic applications, the findings showed how ERI or ERIs can affect the micellar formation of gels composed of 17.5% (w/w) polox407 and 3% (w/w) HPMC or 1% (w/w) NaCMC, important for understating their behaviour and future utilisation as erythrosine delivery systems.
34702525	8	13	theme	qualitative	1304:1314	arg1	composition					1316:1326	qualitative composition	1304:1326	qualitative composition	1304:1326	The systems were studied with respect to theoretical interactions, qualitative composition, morphology, and micellar properties.
34702525	7	14	theme	Optimised	1035:1043	arg1	combinations					1045:1056	Optimised combinations	1035:1056	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC)	1035:1179	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC) were used as micellar systems for ERI or ERIs delivery.
34702525	7	14	theme	Optimised	1035:1043	arg1	systems					1203:1209	micellar systems	1194:1209	micellar systems for ERI or ERIs delivery	1194:1234	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC) were used as micellar systems for ERI or ERIs delivery.
34702525	6	15	theme	polymer	1017:1023	arg1	mixtures					1025:1032	the binary polymer mixtures	1006:1032	the binary polymer mixtures	1006:1032	The systems were evaluated based on the effect of ERI/ERIs on the micellar structure of the binary polymer mixtures.
34702525	5	16	theme	micellar	797:804	arg1	systems					806:812	mucoadhesive and thermoresponsive micellar systems	763:812	mucoadhesive and thermoresponsive micellar systems	763:812	In this work, mucoadhesive and thermoresponsive micellar systems were prepared containing erythrosine in two states: the native form (ERI) and the disodium salt (ERIs).
34702525	11	17	from	photosensitizer	1671:1685	arg1	form					1704:1707	its monomeric form	1690:1707	its monomeric form	1690:1707	Both systems could convert the photosensitizer in its monomeric form, ensuring photodynamic activity.
34702525	4	18	theme	applied	735:741	arg1	drug					743:746	this topically applied drug	720:746	this topically applied drug	720:746	Moreover, using bioadhesive and thermoresponsive polymers to combine in situ gelation and bioadhesion may enhance retention of this topically applied drug.
34702525	8	19	theme	micellar	1345:1352	arg1	properties					1354:1363	micellar properties	1345:1363	micellar properties	1345:1363	The systems were studied with respect to theoretical interactions, qualitative composition, morphology, and micellar properties.
34702525	0	20	theme	derivative	80:89	arg1	blends					91:96	cellulose derivative blends	70:96	cellulose derivative blends	70:96	The effect of erythrosine-B on the structuration of poloxamer 407 and cellulose derivative blends: In silico modelling supporting experimental studies.
34702525	7	21	theme	micellar	1194:1201	arg1	systems					1203:1209	micellar systems	1194:1209	micellar systems for ERI or ERIs delivery	1194:1234	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC) were used as micellar systems for ERI or ERIs delivery.
34702525	7	21	theme	micellar	1194:1201	arg1	combinations					1045:1056	Optimised combinations	1035:1056	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC)	1035:1179	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC) were used as micellar systems for ERI or ERIs delivery.
34702525	5	22	theme	thermoresponsive	780:795	arg1	systems					806:812	mucoadhesive and thermoresponsive micellar systems	763:812	mucoadhesive and thermoresponsive micellar systems	763:812	In this work, mucoadhesive and thermoresponsive micellar systems were prepared containing erythrosine in two states: the native form (ERI) and the disodium salt (ERIs).
34702525	12	23	theme	micellar	1863:1870	arg1	size					1872:1875	micellar size	1863:1875	micellar size	1863:1875	In these mixtures, crystallinity, critical micellar temperature and enthalpy of polox407 micellisation were reduced, and micellar size, evaluated by transmission electron microscopy (TEM), showed low impact of ERI/ERIs in HPMC preparations.
34702525	10	24	contain	containing	1525:1534	arg2	NaCMC					1536:1540	NaCMC	1536:1540	NaCMC	1536:1540	Systems containing NaCMC displayed a repulsive effect in the presence of erythrosine, due to the polymer's charge density.
34702525	10	24	contain	containing	1525:1534	arg1	Systems					1517:1523	Systems	1517:1523	Systems containing NaCMC	1517:1540	Systems containing NaCMC displayed a repulsive effect in the presence of erythrosine, due to the polymer's charge density.
34702525	13	25	dep	HPMC	2148:2151	arg1	%					2140:2140	polox407 and 3%	2126:2140	polox407 and 3%	2126:2140	Aiming toward photodynamic applications, the findings showed how ERI or ERIs can affect the micellar formation of gels composed of 17.5% (w/w) polox407 and 3% (w/w) HPMC or 1% (w/w) NaCMC, important for understating their behaviour and future utilisation as erythrosine delivery systems.
34702525	1	26	theme	microorganisms	279:292	arg1	inactivation					263:274	the inactivation	259:274	the inactivation of microorganisms	259:292	Erythrosine is a dye approved for medical use that has shown promising photodynamic activity, allowing for the inactivation of microorganisms and activity against malignant cells.
34702525	1	26	theme	microorganisms	279:292	arg1	activity					298:305	activity	298:305	activity against malignant cells	298:329	Erythrosine is a dye approved for medical use that has shown promising photodynamic activity, allowing for the inactivation of microorganisms and activity against malignant cells.
34702525	9	27	theme	In	1366:1367	arg1	modelling					1376:1384	In silico modelling	1366:1384	In silico modelling	1366:1384	In silico modelling indicated a higher interaction of the drug with poly(ethylene oxide) (PEO) than poly(propylene oxide) (PPO) fragments of polox407.
34702525	0	28	theme	poloxamer	52:60	arg1	structuration					35:47	the structuration	31:47	the structuration of poloxamer 407 and cellulose derivative blends	31:96	The effect of erythrosine-B on the structuration of poloxamer 407 and cellulose derivative blends: In silico modelling supporting experimental studies.
34702525	3	29	theme	micellar	515:522	arg1	poloxamers					551:560	poloxamers	551:560	poloxamers	551:560	Therefore, the incorporation of erythrosine in micellar polymeric systems, such as poloxamers, may overcome this limitation.
34702525	3	29	theme	micellar	515:522	arg1	systems					534:540	micellar polymeric systems	515:540	micellar polymeric systems	515:540	Therefore, the incorporation of erythrosine in micellar polymeric systems, such as poloxamers, may overcome this limitation.
34702525	7	30	theme	hydroxypropyl	1144:1156	arg1	methylcellulose					1158:1172	hydroxypropyl methylcellulose	1144:1172	hydroxypropyl methylcellulose (HPMC)	1144:1179	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC) were used as micellar systems for ERI or ERIs delivery.
34702525	7	30	theme	hydroxypropyl	1144:1156	arg1	HPMC					1175:1178	HPMC	1175:1178	HPMC	1175:1178	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC) were used as micellar systems for ERI or ERIs delivery.
34702525	1	31	theme	malignant	315:323	arg1	cells					325:329	malignant cells	315:329	malignant cells	315:329	Erythrosine is a dye approved for medical use that has shown promising photodynamic activity, allowing for the inactivation of microorganisms and activity against malignant cells.
34702525	4	32	dep	in	662:663	arg1	situ					665:668	situ	665:668	situ	665:668	Moreover, using bioadhesive and thermoresponsive polymers to combine in situ gelation and bioadhesion may enhance retention of this topically applied drug.
34702525	12	33	theme	micellar	1785:1792	arg1	temperature					1794:1804	critical micellar temperature	1776:1804	critical micellar temperature	1776:1804	In these mixtures, crystallinity, critical micellar temperature and enthalpy of polox407 micellisation were reduced, and micellar size, evaluated by transmission electron microscopy (TEM), showed low impact of ERI/ERIs in HPMC preparations.
34702525	3	34	theme	erythrosine	500:510	arg1	incorporation					483:495	the incorporation	479:495	the incorporation of erythrosine in micellar polymeric systems, such as poloxamers,	479:561	Therefore, the incorporation of erythrosine in micellar polymeric systems, such as poloxamers, may overcome this limitation.
34702525	4	35	dep	enhance	699:705	arg1	using					603:607	using	603:607	using bioadhesive and thermoresponsive polymers to combine in situ gelation	603:677	Moreover, using bioadhesive and thermoresponsive polymers to combine in situ gelation and bioadhesion may enhance retention of this topically applied drug.
34702525	4	36	theme	thermoresponsive	625:640	arg1	polymers					642:649	bioadhesive and thermoresponsive polymers	609:649	bioadhesive and thermoresponsive polymers	609:649	Moreover, using bioadhesive and thermoresponsive polymers to combine in situ gelation and bioadhesion may enhance retention of this topically applied drug.
34702525	1	37	theme	photodynamic	223:234	arg1	activity					236:243	promising photodynamic activity	213:243	promising photodynamic activity	213:243	Erythrosine is a dye approved for medical use that has shown promising photodynamic activity, allowing for the inactivation of microorganisms and activity against malignant cells.
34702525	12	38	theme	HPMC	1964:1967	arg1	preparations					1969:1980	HPMC preparations	1964:1980	HPMC preparations	1964:1980	In these mixtures, crystallinity, critical micellar temperature and enthalpy of polox407 micellisation were reduced, and micellar size, evaluated by transmission electron microscopy (TEM), showed low impact of ERI/ERIs in HPMC preparations.
34702525	13	39	theme	delivery	2253:2260	arg1	behaviour					2205:2213	their behaviour	2199:2213	their behaviour	2199:2213	Aiming toward photodynamic applications, the findings showed how ERI or ERIs can affect the micellar formation of gels composed of 17.5% (w/w) polox407 and 3% (w/w) HPMC or 1% (w/w) NaCMC, important for understating their behaviour and future utilisation as erythrosine delivery systems.
34702525	13	39	theme	delivery	2253:2260	arg1	utilisation					2226:2236	future utilisation	2219:2236	future utilisation	2219:2236	Aiming toward photodynamic applications, the findings showed how ERI or ERIs can affect the micellar formation of gels composed of 17.5% (w/w) polox407 and 3% (w/w) HPMC or 1% (w/w) NaCMC, important for understating their behaviour and future utilisation as erythrosine delivery systems.
34702525	13	39	theme	delivery	2253:2260	arg1	systems					2262:2268	erythrosine delivery systems	2241:2268	erythrosine delivery systems	2241:2268	Aiming toward photodynamic applications, the findings showed how ERI or ERIs can affect the micellar formation of gels composed of 17.5% (w/w) polox407 and 3% (w/w) HPMC or 1% (w/w) NaCMC, important for understating their behaviour and future utilisation as erythrosine delivery systems.
34702525	7	40	theme	carboxymethylcellulose	1110:1131	arg1	combinations					1045:1056	Optimised combinations	1035:1056	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC)	1035:1179	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC) were used as micellar systems for ERI or ERIs delivery.
34702525	7	40	theme	carboxymethylcellulose	1110:1131	arg1	systems					1203:1209	micellar systems	1194:1209	micellar systems for ERI or ERIs delivery	1194:1234	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC) were used as micellar systems for ERI or ERIs delivery.
34702525	5	41	dep	states	858:863	arg1	ERIs					911:914	ERIs	911:914	ERIs	911:914	In this work, mucoadhesive and thermoresponsive micellar systems were prepared containing erythrosine in two states: the native form (ERI) and the disodium salt (ERIs).
34702525	5	41	dep	states	858:863	arg1	states					858:863	two states	854:863	two states: the native form (ERI) and the disodium salt (ERIs)	854:915	In this work, mucoadhesive and thermoresponsive micellar systems were prepared containing erythrosine in two states: the native form (ERI) and the disodium salt (ERIs).
34702525	5	41	dep	states	858:863	arg1	salt					905:908	the disodium salt	892:908	the disodium salt (ERIs)	892:915	In this work, mucoadhesive and thermoresponsive micellar systems were prepared containing erythrosine in two states: the native form (ERI) and the disodium salt (ERIs).
34702525	5	41	dep	states	858:863	arg1	ERI					883:885	ERI	883:885	ERI	883:885	In this work, mucoadhesive and thermoresponsive micellar systems were prepared containing erythrosine in two states: the native form (ERI) and the disodium salt (ERIs).
34702525	5	41	dep	states	858:863	arg1	form					877:880	the native form	866:880	the native form (ERI)	866:886	In this work, mucoadhesive and thermoresponsive micellar systems were prepared containing erythrosine in two states: the native form (ERI) and the disodium salt (ERIs).
34702525	4	42	theme	bioadhesive	609:619	arg1	polymers					642:649	bioadhesive and thermoresponsive polymers	609:649	bioadhesive and thermoresponsive polymers	609:649	Moreover, using bioadhesive and thermoresponsive polymers to combine in situ gelation and bioadhesion may enhance retention of this topically applied drug.
34702525	2	43	theme	great	344:348	arg1	potential					363:371	the great photodynamic potential	340:371	the great photodynamic potential	340:371	Despite the great photodynamic potential, erythrosine exhibits hydrophilicity, negatively impacting its action in biological membranes.
34702525	7	44	theme	mucoadhesive	1090:1101	arg1	carboxymethylcellulose					1110:1131	mucoadhesive sodium carboxymethylcellulose	1090:1131	mucoadhesive sodium carboxymethylcellulose	1090:1131	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC) were used as micellar systems for ERI or ERIs delivery.
34702525	12	45	theme	ERI/ERIs	1952:1959	arg1	impact					1942:1947	low impact	1938:1947	low impact of ERI/ERIs in HPMC preparations	1938:1980	In these mixtures, crystallinity, critical micellar temperature and enthalpy of polox407 micellisation were reduced, and micellar size, evaluated by transmission electron microscopy (TEM), showed low impact of ERI/ERIs in HPMC preparations.
34702525	1	46	theme	approved	173:180	arg1	Erythrosine					152:162	Erythrosine	152:162	Erythrosine	152:162	Erythrosine is a dye approved for medical use that has shown promising photodynamic activity, allowing for the inactivation of microorganisms and activity against malignant cells.
34702525	1	46	theme	approved	173:180	arg1	dye					169:171	a dye	167:171	a dye approved for medical use that has shown promising photodynamic activity, allowing for the inactivation of microorganisms and activity against malignant cells	167:329	Erythrosine is a dye approved for medical use that has shown promising photodynamic activity, allowing for the inactivation of microorganisms and activity against malignant cells.
34702525	10	47	theme	repulsive	1554:1562	arg1	effect					1564:1569	a repulsive effect	1552:1569	a repulsive effect	1552:1569	Systems containing NaCMC displayed a repulsive effect in the presence of erythrosine, due to the polymer's charge density.
34702525	1	48	theme	medical	186:192	arg1	use					194:196	medical use	186:196	medical use that has shown promising photodynamic activity, allowing for the inactivation of microorganisms and activity against malignant cells	186:329	Erythrosine is a dye approved for medical use that has shown promising photodynamic activity, allowing for the inactivation of microorganisms and activity against malignant cells.
34702525	4	49	theme	in	662:663	arg1	gelation					670:677	in situ gelation	662:677	in situ gelation	662:677	Moreover, using bioadhesive and thermoresponsive polymers to combine in situ gelation and bioadhesion may enhance retention of this topically applied drug.
34702525	10	50	theme	erythrosine	1590:1600	arg1	presence					1578:1585	the presence	1574:1585	the presence of erythrosine	1574:1600	Systems containing NaCMC displayed a repulsive effect in the presence of erythrosine, due to the polymer's charge density.
34702525	0	51	theme	experimental	130:141	arg1	studies					143:149	experimental studies	130:149	experimental studies	130:149	The effect of erythrosine-B on the structuration of poloxamer 407 and cellulose derivative blends: In silico modelling supporting experimental studies.
34702525	13	52	theme	future	2219:2224	arg1	behaviour					2205:2213	their behaviour	2199:2213	their behaviour	2199:2213	Aiming toward photodynamic applications, the findings showed how ERI or ERIs can affect the micellar formation of gels composed of 17.5% (w/w) polox407 and 3% (w/w) HPMC or 1% (w/w) NaCMC, important for understating their behaviour and future utilisation as erythrosine delivery systems.
34702525	13	52	theme	future	2219:2224	arg1	utilisation					2226:2236	future utilisation	2219:2236	future utilisation	2219:2236	Aiming toward photodynamic applications, the findings showed how ERI or ERIs can affect the micellar formation of gels composed of 17.5% (w/w) polox407 and 3% (w/w) HPMC or 1% (w/w) NaCMC, important for understating their behaviour and future utilisation as erythrosine delivery systems.
34702525	13	52	theme	future	2219:2224	arg1	systems					2262:2268	erythrosine delivery systems	2241:2268	erythrosine delivery systems	2241:2268	Aiming toward photodynamic applications, the findings showed how ERI or ERIs can affect the micellar formation of gels composed of 17.5% (w/w) polox407 and 3% (w/w) HPMC or 1% (w/w) NaCMC, important for understating their behaviour and future utilisation as erythrosine delivery systems.
34702525	12	53	theme	electron	1904:1911	arg1	TEM					1925:1927	TEM	1925:1927	TEM	1925:1927	In these mixtures, crystallinity, critical micellar temperature and enthalpy of polox407 micellisation were reduced, and micellar size, evaluated by transmission electron microscopy (TEM), showed low impact of ERI/ERIs in HPMC preparations.
34702525	12	53	theme	electron	1904:1911	arg1	microscopy					1913:1922	transmission electron microscopy	1891:1922	transmission electron microscopy (TEM)	1891:1928	In these mixtures, crystallinity, critical micellar temperature and enthalpy of polox407 micellisation were reduced, and micellar size, evaluated by transmission electron microscopy (TEM), showed low impact of ERI/ERIs in HPMC preparations.
34702525	9	54	theme	poly	1466:1469	arg1	fragments					1494:1502	poly(propylene oxide) (PPO) fragments	1466:1502	poly(propylene oxide) (PPO) fragments of polox407	1466:1514	In silico modelling indicated a higher interaction of the drug with poly(ethylene oxide) (PEO) than poly(propylene oxide) (PPO) fragments of polox407.
34702525	8	55	theme	theoretical	1278:1288	arg1	interactions					1290:1301	theoretical interactions	1278:1301	theoretical interactions	1278:1301	The systems were studied with respect to theoretical interactions, qualitative composition, morphology, and micellar properties.
34702525	9	56	theme	propylene	1471:1479	arg1	poly					1466:1469	poly	1466:1469	poly(propylene oxide) (PPO) fragments of polox407	1466:1514	In silico modelling indicated a higher interaction of the drug with poly(ethylene oxide) (PEO) than poly(propylene oxide) (PPO) fragments of polox407.
34702525	9	56	theme	propylene	1471:1479	arg1	oxide					1481:1485	propylene oxide	1471:1485	propylene oxide	1471:1485	In silico modelling indicated a higher interaction of the drug with poly(ethylene oxide) (PEO) than poly(propylene oxide) (PPO) fragments of polox407.
34702525	6	57	theme	mixtures	1025:1032	arg1	structure					993:1001	the micellar structure	980:1001	the micellar structure of the binary polymer mixtures	980:1032	The systems were evaluated based on the effect of ERI/ERIs on the micellar structure of the binary polymer mixtures.
34702525	7	58	theme	ERIs	1222:1225	arg1	delivery					1227:1234	ERIs delivery	1222:1234	ERIs delivery	1222:1234	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC) were used as micellar systems for ERI or ERIs delivery.
34702525	5	59	theme	native	870:875	arg1	states					858:863	two states	854:863	two states: the native form (ERI) and the disodium salt (ERIs)	854:915	In this work, mucoadhesive and thermoresponsive micellar systems were prepared containing erythrosine in two states: the native form (ERI) and the disodium salt (ERIs).
34702525	5	59	theme	native	870:875	arg1	ERI					883:885	ERI	883:885	ERI	883:885	In this work, mucoadhesive and thermoresponsive micellar systems were prepared containing erythrosine in two states: the native form (ERI) and the disodium salt (ERIs).
34702525	5	59	theme	native	870:875	arg1	form					877:880	the native form	866:880	the native form (ERI)	866:886	In this work, mucoadhesive and thermoresponsive micellar systems were prepared containing erythrosine in two states: the native form (ERI) and the disodium salt (ERIs).
34702525	13	60	theme	HPMC	2148:2151	arg1	%					2118:2118	17.5%	2114:2118	17.5% (w/w) polox407 and 3% (w/w) HPMC	2114:2151	Aiming toward photodynamic applications, the findings showed how ERI or ERIs can affect the micellar formation of gels composed of 17.5% (w/w) polox407 and 3% (w/w) HPMC or 1% (w/w) NaCMC, important for understating their behaviour and future utilisation as erythrosine delivery systems.
34702525	13	60	theme	HPMC	2148:2151	arg1	w/w					2121:2123	w/w	2121:2123	w/w	2121:2123	Aiming toward photodynamic applications, the findings showed how ERI or ERIs can affect the micellar formation of gels composed of 17.5% (w/w) polox407 and 3% (w/w) HPMC or 1% (w/w) NaCMC, important for understating their behaviour and future utilisation as erythrosine delivery systems.
34702525	6	61	theme	binary	1010:1015	arg1	mixtures					1025:1032	the binary polymer mixtures	1006:1032	the binary polymer mixtures	1006:1032	The systems were evaluated based on the effect of ERI/ERIs on the micellar structure of the binary polymer mixtures.
34702525	11	62	theme	monomeric	1694:1702	arg1	form					1704:1707	its monomeric form	1690:1707	its monomeric form	1690:1707	Both systems could convert the photosensitizer in its monomeric form, ensuring photodynamic activity.
34702525	0	63	theme	cellulose	70:78	arg1	blends					91:96	cellulose derivative blends	70:96	cellulose derivative blends	70:96	The effect of erythrosine-B on the structuration of poloxamer 407 and cellulose derivative blends: In silico modelling supporting experimental studies.
34702525	0	64	from	effect	4:9	arg1	structuration					35:47	the structuration	31:47	the structuration of poloxamer 407 and cellulose derivative blends	31:96	The effect of erythrosine-B on the structuration of poloxamer 407 and cellulose derivative blends: In silico modelling supporting experimental studies.
34702525	0	65	theme	blends	91:96	arg1	structuration					35:47	the structuration	31:47	the structuration of poloxamer 407 and cellulose derivative blends	31:96	The effect of erythrosine-B on the structuration of poloxamer 407 and cellulose derivative blends: In silico modelling supporting experimental studies.
34702525	12	66	theme	transmission	1891:1902	arg1	TEM					1925:1927	TEM	1925:1927	TEM	1925:1927	In these mixtures, crystallinity, critical micellar temperature and enthalpy of polox407 micellisation were reduced, and micellar size, evaluated by transmission electron microscopy (TEM), showed low impact of ERI/ERIs in HPMC preparations.
34702525	12	66	theme	transmission	1891:1902	arg1	microscopy					1913:1922	transmission electron microscopy	1891:1922	transmission electron microscopy (TEM)	1891:1928	In these mixtures, crystallinity, critical micellar temperature and enthalpy of polox407 micellisation were reduced, and micellar size, evaluated by transmission electron microscopy (TEM), showed low impact of ERI/ERIs in HPMC preparations.
34702525	0	67	dep	In	99:100	arg1	silico					102:107	silico	102:107	silico	102:107	The effect of erythrosine-B on the structuration of poloxamer 407 and cellulose derivative blends: In silico modelling supporting experimental studies.
34702525	6	68	theme	micellar	984:991	arg1	structure					993:1001	the micellar structure	980:1001	the micellar structure of the binary polymer mixtures	980:1032	The systems were evaluated based on the effect of ERI/ERIs on the micellar structure of the binary polymer mixtures.
34702525	11	69	theme	photodynamic	1719:1730	arg1	activity					1732:1739	photodynamic activity	1719:1739	photodynamic activity	1719:1739	Both systems could convert the photosensitizer in its monomeric form, ensuring photodynamic activity.
34702525	0	70	theme	In	99:100	arg1	modelling					109:117	In silico modelling	99:117	The effect of erythrosine-B on the structuration of poloxamer 407 and cellulose derivative blends: In silico modelling supporting experimental studies.	0:150	The effect of erythrosine-B on the structuration of poloxamer 407 and cellulose derivative blends: In silico modelling supporting experimental studies.
34702525	13	71	theme	photodynamic	1997:2008	arg1	applications					2010:2021	photodynamic applications	1997:2021	photodynamic applications	1997:2021	Aiming toward photodynamic applications, the findings showed how ERI or ERIs can affect the micellar formation of gels composed of 17.5% (w/w) polox407 and 3% (w/w) HPMC or 1% (w/w) NaCMC, important for understating their behaviour and future utilisation as erythrosine delivery systems.
34702525	9	72	theme	polox407	1507:1514	arg1	fragments					1494:1502	poly(propylene oxide) (PPO) fragments	1466:1502	poly(propylene oxide) (PPO) fragments of polox407	1466:1514	In silico modelling indicated a higher interaction of the drug with poly(ethylene oxide) (PEO) than poly(propylene oxide) (PPO) fragments of polox407.
34702525	9	73	dep	In	1366:1367	arg1	silico					1369:1374	silico	1369:1374	silico	1369:1374	In silico modelling indicated a higher interaction of the drug with poly(ethylene oxide) (PEO) than poly(propylene oxide) (PPO) fragments of polox407.
34702525	1	74	theme	promising	213:221	arg1	activity					236:243	promising photodynamic activity	213:243	promising photodynamic activity	213:243	Erythrosine is a dye approved for medical use that has shown promising photodynamic activity, allowing for the inactivation of microorganisms and activity against malignant cells.
34702525	5	75	theme	mucoadhesive	763:774	arg1	systems					806:812	mucoadhesive and thermoresponsive micellar systems	763:812	mucoadhesive and thermoresponsive micellar systems	763:812	In this work, mucoadhesive and thermoresponsive micellar systems were prepared containing erythrosine in two states: the native form (ERI) and the disodium salt (ERIs).
34702525	7	76	theme	poloxamer	1061:1069	arg1	combinations					1045:1056	Optimised combinations	1035:1056	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC)	1035:1179	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC) were used as micellar systems for ERI or ERIs delivery.
34702525	7	76	theme	poloxamer	1061:1069	arg1	systems					1203:1209	micellar systems	1194:1209	micellar systems for ERI or ERIs delivery	1194:1234	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC) were used as micellar systems for ERI or ERIs delivery.
34702525	0	77	theme	erythrosine-B	14:26	arg1	effect					4:9	The effect	0:9	The effect of erythrosine-B on the structuration of poloxamer 407 and cellulose derivative blends: In silico modelling supporting experimental studies.	0:150	The effect of erythrosine-B on the structuration of poloxamer 407 and cellulose derivative blends: In silico modelling supporting experimental studies.
34702525	0	78	dep	effect	4:9	arg1	modelling					109:117	In silico modelling	99:117	The effect of erythrosine-B on the structuration of poloxamer 407 and cellulose derivative blends: In silico modelling supporting experimental studies.	0:150	The effect of erythrosine-B on the structuration of poloxamer 407 and cellulose derivative blends: In silico modelling supporting experimental studies.
34702525	12	79	theme	micellisation	1831:1843	arg1	enthalpy					1810:1817	enthalpy	1810:1817	enthalpy	1810:1817	In these mixtures, crystallinity, critical micellar temperature and enthalpy of polox407 micellisation were reduced, and micellar size, evaluated by transmission electron microscopy (TEM), showed low impact of ERI/ERIs in HPMC preparations.
34702525	12	79	theme	micellisation	1831:1843	arg1	temperature					1794:1804	critical micellar temperature	1776:1804	critical micellar temperature	1776:1804	In these mixtures, crystallinity, critical micellar temperature and enthalpy of polox407 micellisation were reduced, and micellar size, evaluated by transmission electron microscopy (TEM), showed low impact of ERI/ERIs in HPMC preparations.
34702525	12	79	theme	micellisation	1831:1843	arg1	crystallinity					1761:1773	crystallinity	1761:1773	crystallinity	1761:1773	In these mixtures, crystallinity, critical micellar temperature and enthalpy of polox407 micellisation were reduced, and micellar size, evaluated by transmission electron microscopy (TEM), showed low impact of ERI/ERIs in HPMC preparations.
34702525	2	80	theme	biological	446:455	arg1	membranes					457:465	biological membranes	446:465	biological membranes	446:465	Despite the great photodynamic potential, erythrosine exhibits hydrophilicity, negatively impacting its action in biological membranes.
34702525	6	81	from	effect	958:963	arg1	structure					993:1001	the micellar structure	980:1001	the micellar structure of the binary polymer mixtures	980:1032	The systems were evaluated based on the effect of ERI/ERIs on the micellar structure of the binary polymer mixtures.
34702525	10	82	theme	charge	1624:1629	arg1	density					1631:1637	the polymer's charge density	1610:1637	the polymer's charge density	1610:1637	Systems containing NaCMC displayed a repulsive effect in the presence of erythrosine, due to the polymer's charge density.
34702525	7	83	theme	methylcellulose	1158:1172	arg1	combinations					1045:1056	Optimised combinations	1035:1056	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC)	1035:1179	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC) were used as micellar systems for ERI or ERIs delivery.
34702525	7	83	theme	methylcellulose	1158:1172	arg1	systems					1203:1209	micellar systems	1194:1209	micellar systems for ERI or ERIs delivery	1194:1234	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC) were used as micellar systems for ERI or ERIs delivery.
34702525	7	84	used	used	1186:1189	arg2	systems					1203:1209	micellar systems	1194:1209	micellar systems for ERI or ERIs delivery	1194:1234	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC) were used as micellar systems for ERI or ERIs delivery.
34702525	7	84	used	used	1186:1189	arg2	combinations					1045:1056	Optimised combinations	1035:1056	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC)	1035:1179	Optimised combinations of poloxamer 407 (polox407) and mucoadhesive sodium carboxymethylcellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC) were used as micellar systems for ERI or ERIs delivery.
34702525	3	85	from	incorporation	483:495	arg1	poloxamers					551:560	poloxamers	551:560	poloxamers	551:560	Therefore, the incorporation of erythrosine in micellar polymeric systems, such as poloxamers, may overcome this limitation.
34702525	3	85	from	incorporation	483:495	arg1	systems					534:540	micellar polymeric systems	515:540	micellar polymeric systems	515:540	Therefore, the incorporation of erythrosine in micellar polymeric systems, such as poloxamers, may overcome this limitation.
34702525	3	86	theme	polymeric	524:532	arg1	poloxamers					551:560	poloxamers	551:560	poloxamers	551:560	Therefore, the incorporation of erythrosine in micellar polymeric systems, such as poloxamers, may overcome this limitation.
34702525	3	86	theme	polymeric	524:532	arg1	systems					534:540	micellar polymeric systems	515:540	micellar polymeric systems	515:540	Therefore, the incorporation of erythrosine in micellar polymeric systems, such as poloxamers, may overcome this limitation.
34702525	12	87	theme	critical	1776:1783	arg1	temperature					1794:1804	critical micellar temperature	1776:1804	critical micellar temperature	1776:1804	In these mixtures, crystallinity, critical micellar temperature and enthalpy of polox407 micellisation were reduced, and micellar size, evaluated by transmission electron microscopy (TEM), showed low impact of ERI/ERIs in HPMC preparations.
34702525	9	88	with	interaction	1405:1415	arg1	oxide					1448:1452	ethylene oxide	1439:1452	ethylene oxide	1439:1452	In silico modelling indicated a higher interaction of the drug with poly(ethylene oxide) (PEO) than poly(propylene oxide) (PPO) fragments of polox407.
34702525	9	88	with	interaction	1405:1415	arg1	PEO					1456:1458	PEO	1456:1458	PEO	1456:1458	In silico modelling indicated a higher interaction of the drug with poly(ethylene oxide) (PEO) than poly(propylene oxide) (PPO) fragments of polox407.
34702525	9	88	with	interaction	1405:1415	arg1	poly					1434:1437	poly	1434:1437	poly(ethylene oxide) (PEO)	1434:1459	In silico modelling indicated a higher interaction of the drug with poly(ethylene oxide) (PEO) than poly(propylene oxide) (PPO) fragments of polox407.
34702525	9	89	theme	higher	1398:1403	arg1	interaction					1405:1415	a higher interaction	1396:1415	a higher interaction of the drug with poly(ethylene oxide) (PEO)	1396:1459	In silico modelling indicated a higher interaction of the drug with poly(ethylene oxide) (PEO) than poly(propylene oxide) (PPO) fragments of polox407.
34702525	13	90	theme	erythrosine	2241:2251	arg1	behaviour					2205:2213	their behaviour	2199:2213	their behaviour	2199:2213	Aiming toward photodynamic applications, the findings showed how ERI or ERIs can affect the micellar formation of gels composed of 17.5% (w/w) polox407 and 3% (w/w) HPMC or 1% (w/w) NaCMC, important for understating their behaviour and future utilisation as erythrosine delivery systems.
34702525	13	90	theme	erythrosine	2241:2251	arg1	utilisation					2226:2236	future utilisation	2219:2236	future utilisation	2219:2236	Aiming toward photodynamic applications, the findings showed how ERI or ERIs can affect the micellar formation of gels composed of 17.5% (w/w) polox407 and 3% (w/w) HPMC or 1% (w/w) NaCMC, important for understating their behaviour and future utilisation as erythrosine delivery systems.
34702525	13	90	theme	erythrosine	2241:2251	arg1	systems					2262:2268	erythrosine delivery systems	2241:2268	erythrosine delivery systems	2241:2268	Aiming toward photodynamic applications, the findings showed how ERI or ERIs can affect the micellar formation of gels composed of 17.5% (w/w) polox407 and 3% (w/w) HPMC or 1% (w/w) NaCMC, important for understating their behaviour and future utilisation as erythrosine delivery systems.
32791271	5	0	theme	composite	832:840	arg1	sponge					842:847	the chitosan/cellulose composite sponge	809:847	the chitosan/cellulose composite sponge	809:847	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
32791271	7	1	theme	massive	1211:1217	arg1	haemorrhage					1219:1229	massive haemorrhage	1211:1229	massive haemorrhage	1211:1229	Better biocompatibility of the composite sponge is proved by the results of hemocompatibility and cytotoxicity, indicating an excellent candidate for rapid hemostasis in massive haemorrhage.
32791271	2	2	theme	poor	390:393	arg1	softness					395:402	poor softness	390:402	poor softness	390:402	The preparation of composite sponge by LiOH/KOH/urea solvent system effectively solve the problems of uneven distribution of chitosan, poor softness and acid residue caused by soaking in chitosan acid solution.
32791271	7	3	theme	Better	1041:1046	arg1	biocompatibility					1048:1063	Better biocompatibility	1041:1063	Better biocompatibility of the composite sponge	1041:1087	Better biocompatibility of the composite sponge is proved by the results of hemocompatibility and cytotoxicity, indicating an excellent candidate for rapid hemostasis in massive haemorrhage.
32791271	1	4	theme	hydrophilic	160:170	arg1	sponge					191:196	porous and hydrophilic chitosan/cellulose sponge	149:196	porous and hydrophilic chitosan/cellulose sponge using surfactant and pore-forming agent	149:236	Here, a simple and efficient strategy to produce porous and hydrophilic chitosan/cellulose sponge using surfactant and pore-forming agent is demonstrated.
32791271	5	5	theme	clotting	780:787	arg1	time					789:792	the dynamic whole blood clotting time	756:792	the dynamic whole blood clotting time	756:792	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
32791271	1	6	dep	strategy	129:136	arg1	produce					141:147	produce	141:147	to produce porous and hydrophilic chitosan/cellulose sponge using surfactant and pore-forming agent	138:236	Here, a simple and efficient strategy to produce porous and hydrophilic chitosan/cellulose sponge using surfactant and pore-forming agent is demonstrated.
32791271	5	7	theme	time	789:792	arg1	result					746:751	the result	742:751	the result of the dynamic whole blood clotting time	742:792	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
32791271	3	8	theme	good	588:591	arg1	properties					604:613	good mechanical properties	588:613	good mechanical properties	588:613	The obtained chitosan/cellulose sponges exhibit high water absorption capacity and rapid shape recoverability, as well as good mechanical properties.
32791271	3	9	theme	rapid	549:553	arg1	recoverability					561:574	rapid shape recoverability	549:574	rapid shape recoverability	549:574	The obtained chitosan/cellulose sponges exhibit high water absorption capacity and rapid shape recoverability, as well as good mechanical properties.
32791271	3	10	theme	absorption	525:534	arg1	capacity					536:543	high water absorption capacity	514:543	high water absorption capacity	514:543	The obtained chitosan/cellulose sponges exhibit high water absorption capacity and rapid shape recoverability, as well as good mechanical properties.
32791271	6	11	theme	rapid	970:974	arg1	hemostasis					976:985	rapid hemostasis	970:985	rapid hemostasis within 34 s	970:997	Animal experiment further showed that rapid hemostasis within 34 s can be reached with the composite sponge.
32791271	2	12	theme	chitosan	442:449	arg1	solution					456:463	chitosan acid solution	442:463	chitosan acid solution	442:463	The preparation of composite sponge by LiOH/KOH/urea solvent system effectively solve the problems of uneven distribution of chitosan, poor softness and acid residue caused by soaking in chitosan acid solution.
32791271	2	13	theme	solvent	308:314	arg1	system					316:321	LiOH/KOH/urea solvent system	294:321	LiOH/KOH/urea solvent system	294:321	The preparation of composite sponge by LiOH/KOH/urea solvent system effectively solve the problems of uneven distribution of chitosan, poor softness and acid residue caused by soaking in chitosan acid solution.
32791271	1	14	theme	chitosan/cellulose	172:189	arg1	sponge					191:196	porous and hydrophilic chitosan/cellulose sponge	149:196	porous and hydrophilic chitosan/cellulose sponge using surfactant and pore-forming agent	149:236	Here, a simple and efficient strategy to produce porous and hydrophilic chitosan/cellulose sponge using surfactant and pore-forming agent is demonstrated.
32791271	7	15	theme	rapid	1191:1195	arg1	hemostasis					1197:1206	rapid hemostasis	1191:1206	rapid hemostasis in massive haemorrhage	1191:1229	Better biocompatibility of the composite sponge is proved by the results of hemocompatibility and cytotoxicity, indicating an excellent candidate for rapid hemostasis in massive haemorrhage.
32791271	2	16	theme	LiOH/KOH/urea	294:306	arg1	system					316:321	LiOH/KOH/urea solvent system	294:321	LiOH/KOH/urea solvent system	294:321	The preparation of composite sponge by LiOH/KOH/urea solvent system effectively solve the problems of uneven distribution of chitosan, poor softness and acid residue caused by soaking in chitosan acid solution.
32791271	3	17	theme	obtained	470:477	arg1	sponges					498:504	The obtained chitosan/cellulose sponges	466:504	The obtained chitosan/cellulose sponges	466:504	The obtained chitosan/cellulose sponges exhibit high water absorption capacity and rapid shape recoverability, as well as good mechanical properties.
32791271	7	18	theme	excellent	1167:1175	arg1	candidate					1177:1185	an excellent candidate	1164:1185	an excellent candidate for rapid hemostasis in massive haemorrhage	1164:1229	Better biocompatibility of the composite sponge is proved by the results of hemocompatibility and cytotoxicity, indicating an excellent candidate for rapid hemostasis in massive haemorrhage.
32791271	2	19	theme	residue	413:419	arg1	distribution					364:375	uneven distribution	357:375	uneven distribution of chitosan, poor softness and acid residue caused by soaking in chitosan acid solution	357:463	The preparation of composite sponge by LiOH/KOH/urea solvent system effectively solve the problems of uneven distribution of chitosan, poor softness and acid residue caused by soaking in chitosan acid solution.
32791271	7	20	theme	cytotoxicity	1139:1150	arg1	results					1106:1112	the results	1102:1112	the results of hemocompatibility and cytotoxicity	1102:1150	Better biocompatibility of the composite sponge is proved by the results of hemocompatibility and cytotoxicity, indicating an excellent candidate for rapid hemostasis in massive haemorrhage.
32791271	2	21	theme	acid	408:411	arg1	residue					413:419	acid residue	408:419	acid residue	408:419	The preparation of composite sponge by LiOH/KOH/urea solvent system effectively solve the problems of uneven distribution of chitosan, poor softness and acid residue caused by soaking in chitosan acid solution.
32791271	0	22	theme	composite	36:44	arg1	sponge					46:51	composite sponge	36:51	composite sponge	36:51	Rapid hemostatic chitosan/cellulose composite sponge by alkali/urea method for massive haemorrhage.
32791271	3	23	theme	mechanical	593:602	arg1	properties					604:613	good mechanical properties	588:613	good mechanical properties	588:613	The obtained chitosan/cellulose sponges exhibit high water absorption capacity and rapid shape recoverability, as well as good mechanical properties.
32791271	2	24	theme	acid	451:454	arg1	solution					456:463	chitosan acid solution	442:463	chitosan acid solution	442:463	The preparation of composite sponge by LiOH/KOH/urea solvent system effectively solve the problems of uneven distribution of chitosan, poor softness and acid residue caused by soaking in chitosan acid solution.
32791271	5	25	theme	traditional	894:904	arg1	gauze					906:910	traditional gauze	894:910	traditional gauze	894:910	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
32791271	5	26	theme	dynamic	760:766	arg1	time					789:792	the dynamic whole blood clotting time	756:792	the dynamic whole blood clotting time	756:792	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
32791271	1	27	theme	simple	108:113	arg1	strategy					129:136	a simple and efficient strategy	106:136	a simple and efficient strategy to produce porous and hydrophilic chitosan/cellulose sponge using surfactant and pore-forming agent	106:236	Here, a simple and efficient strategy to produce porous and hydrophilic chitosan/cellulose sponge using surfactant and pore-forming agent is demonstrated.
32791271	1	28	theme	pore-forming	219:230	arg1	agent					232:236	surfactant and pore-forming agent	204:236	surfactant and pore-forming agent	204:236	Here, a simple and efficient strategy to produce porous and hydrophilic chitosan/cellulose sponge using surfactant and pore-forming agent is demonstrated.
32791271	5	29	theme	whole	768:772	arg1	time					789:792	the dynamic whole blood clotting time	756:792	the dynamic whole blood clotting time	756:792	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
32791271	5	30	theme	gelatin	916:922	arg1	sponge					924:929	gelatin sponge	916:929	gelatin sponge	916:929	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
32791271	1	31	theme	surfactant	204:213	arg1	agent					232:236	surfactant and pore-forming agent	204:236	surfactant and pore-forming agent	204:236	Here, a simple and efficient strategy to produce porous and hydrophilic chitosan/cellulose sponge using surfactant and pore-forming agent is demonstrated.
32791271	3	32	theme	chitosan/cellulose	479:496	arg1	sponges					498:504	The obtained chitosan/cellulose sponges	466:504	The obtained chitosan/cellulose sponges	466:504	The obtained chitosan/cellulose sponges exhibit high water absorption capacity and rapid shape recoverability, as well as good mechanical properties.
32791271	5	33	theme	better	853:858	arg1	ability					872:878	better coagulation ability	853:878	better coagulation ability	853:878	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
32791271	6	34	theme	composite	1023:1031	arg1	sponge					1033:1038	the composite sponge	1019:1038	the composite sponge	1019:1038	Animal experiment further showed that rapid hemostasis within 34 s can be reached with the composite sponge.
32791271	5	35	theme	blood	774:778	arg1	time					789:792	the dynamic whole blood clotting time	756:792	the dynamic whole blood clotting time	756:792	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
32791271	6	36	theme	Animal	932:937	arg1	experiment					939:948	Animal experiment	932:948	Animal experiment	932:948	Animal experiment further showed that rapid hemostasis within 34 s can be reached with the composite sponge.
32791271	5	37	theme	coagulation	860:870	arg1	ability					872:878	better coagulation ability	853:878	better coagulation ability	853:878	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
32791271	7	38	from	hemostasis	1197:1206	arg1	haemorrhage					1219:1229	massive haemorrhage	1211:1229	massive haemorrhage	1211:1229	Better biocompatibility of the composite sponge is proved by the results of hemocompatibility and cytotoxicity, indicating an excellent candidate for rapid hemostasis in massive haemorrhage.
32791271	7	39	theme	hemocompatibility	1117:1133	arg1	results					1106:1112	the results	1102:1112	the results of hemocompatibility and cytotoxicity	1102:1150	Better biocompatibility of the composite sponge is proved by the results of hemocompatibility and cytotoxicity, indicating an excellent candidate for rapid hemostasis in massive haemorrhage.
32791271	2	40	theme	chitosan	380:387	arg1	distribution					364:375	uneven distribution	357:375	uneven distribution of chitosan, poor softness and acid residue caused by soaking in chitosan acid solution	357:463	The preparation of composite sponge by LiOH/KOH/urea solvent system effectively solve the problems of uneven distribution of chitosan, poor softness and acid residue caused by soaking in chitosan acid solution.
32791271	0	41	theme	alkali/urea	56:66	arg1	method					68:73	alkali/urea method	56:73	alkali/urea method for massive haemorrhage	56:97	Rapid hemostatic chitosan/cellulose composite sponge by alkali/urea method for massive haemorrhage.
32791271	1	42	theme	porous	149:154	arg1	sponge					191:196	porous and hydrophilic chitosan/cellulose sponge	149:196	porous and hydrophilic chitosan/cellulose sponge using surfactant and pore-forming agent	149:236	Here, a simple and efficient strategy to produce porous and hydrophilic chitosan/cellulose sponge using surfactant and pore-forming agent is demonstrated.
32791271	3	43	theme	shape	555:559	arg1	recoverability					561:574	rapid shape recoverability	549:574	rapid shape recoverability	549:574	The obtained chitosan/cellulose sponges exhibit high water absorption capacity and rapid shape recoverability, as well as good mechanical properties.
32791271	1	44	theme	efficient	119:127	arg1	strategy					129:136	a simple and efficient strategy	106:136	a simple and efficient strategy to produce porous and hydrophilic chitosan/cellulose sponge using surfactant and pore-forming agent	106:236	Here, a simple and efficient strategy to produce porous and hydrophilic chitosan/cellulose sponge using surfactant and pore-forming agent is demonstrated.
32791271	2	45	theme	distribution	364:375	arg1	problems					345:352	the problems	341:352	the problems of uneven distribution of chitosan, poor softness and acid residue caused by soaking in chitosan acid solution	341:463	The preparation of composite sponge by LiOH/KOH/urea solvent system effectively solve the problems of uneven distribution of chitosan, poor softness and acid residue caused by soaking in chitosan acid solution.
32791271	2	46	theme	sponge	284:289	arg1	preparation					259:269	The preparation	255:269	The preparation of composite sponge by LiOH/KOH/urea solvent system	255:321	The preparation of composite sponge by LiOH/KOH/urea solvent system effectively solve the problems of uneven distribution of chitosan, poor softness and acid residue caused by soaking in chitosan acid solution.
32791271	7	47	theme	composite	1072:1080	arg1	sponge					1082:1087	the composite sponge	1068:1087	the composite sponge	1068:1087	Better biocompatibility of the composite sponge is proved by the results of hemocompatibility and cytotoxicity, indicating an excellent candidate for rapid hemostasis in massive haemorrhage.
32791271	2	48	theme	uneven	357:362	arg1	distribution					364:375	uneven distribution	357:375	uneven distribution of chitosan, poor softness and acid residue caused by soaking in chitosan acid solution	357:463	The preparation of composite sponge by LiOH/KOH/urea solvent system effectively solve the problems of uneven distribution of chitosan, poor softness and acid residue caused by soaking in chitosan acid solution.
32791271	3	49	theme	high	514:517	arg1	capacity					536:543	high water absorption capacity	514:543	high water absorption capacity	514:543	The obtained chitosan/cellulose sponges exhibit high water absorption capacity and rapid shape recoverability, as well as good mechanical properties.
32791271	3	50	theme	water	519:523	arg1	capacity					536:543	high water absorption capacity	514:543	high water absorption capacity	514:543	The obtained chitosan/cellulose sponges exhibit high water absorption capacity and rapid shape recoverability, as well as good mechanical properties.
32791271	0	51	theme	massive	79:85	arg1	haemorrhage					87:97	massive haemorrhage	79:97	massive haemorrhage	79:97	Rapid hemostatic chitosan/cellulose composite sponge by alkali/urea method for massive haemorrhage.
32791271	5	52	contain	has	849:851	arg2	ability					872:878	better coagulation ability	853:878	better coagulation ability	853:878	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
32791271	5	52	contain	has	849:851	arg1	sponge					842:847	the chitosan/cellulose composite sponge	809:847	the chitosan/cellulose composite sponge	809:847	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
32791271	2	53	theme	composite	274:282	arg1	sponge					284:289	composite sponge	274:289	composite sponge	274:289	The preparation of composite sponge by LiOH/KOH/urea solvent system effectively solve the problems of uneven distribution of chitosan, poor softness and acid residue caused by soaking in chitosan acid solution.
32791271	7	54	theme	sponge	1082:1087	arg1	biocompatibility					1048:1063	Better biocompatibility	1041:1063	Better biocompatibility of the composite sponge	1041:1087	Better biocompatibility of the composite sponge is proved by the results of hemocompatibility and cytotoxicity, indicating an excellent candidate for rapid hemostasis in massive haemorrhage.
32791271	5	55	theme	chitosan/cellulose	813:830	arg1	sponge					842:847	the chitosan/cellulose composite sponge	809:847	the chitosan/cellulose composite sponge	809:847	Besides, the result of the dynamic whole blood clotting time indicated that the chitosan/cellulose composite sponge has better coagulation ability than those of traditional gauze and gelatin sponge.
32791271	2	56	theme	softness	395:402	arg1	distribution					364:375	uneven distribution	357:375	uneven distribution of chitosan, poor softness and acid residue caused by soaking in chitosan acid solution	357:463	The preparation of composite sponge by LiOH/KOH/urea solvent system effectively solve the problems of uneven distribution of chitosan, poor softness and acid residue caused by soaking in chitosan acid solution.
32810535	6	0	from	schrenckii	960:969	arg1	CS					942:943	CS	942:943	CS from Acipenser schrenckii	942:969	Bioactivity studies, including MTT assay and scratch-wound assays revealed that CS from Acipenser schrenckii had significant proliferation activity.
32810535	2	1	from	fish	288:291	arg1	glycosaminoglycans					257:274	glycosaminoglycans	257:274	glycosaminoglycans from marine fish	257:291	To explore glycosaminoglycans from marine fish, polysaccharides from the cartilage of the sturgeon, Acipenser schrenckii, were extracted.
32810535	0	2	theme	sturgeon	87:94	arg1	schrenckii					107:116	the sturgeon, Acipenser schrenckii	83:116	schrenckii	107:116	Structural characterization and proliferation activity of chondroitin sulfate from the sturgeon, Acipenser schrenckii.
32810535	0	3	from	characterization	11:26	arg1	schrenckii					107:116	the sturgeon, Acipenser schrenckii	83:116	schrenckii	107:116	Structural characterization and proliferation activity of chondroitin sulfate from the sturgeon, Acipenser schrenckii.
32810535	7	4	theme	Fibroblast	1082:1091	arg1	factor					1100:1105	Fibroblast growth factor 2	1082:1107	Fibroblast growth factor 2 (FGF2)	1082:1114	The proliferation activity of the polysaccharide, YG-1, was related to Fibroblast growth factor 2 (FGF2).
32810535	7	4	theme	Fibroblast	1082:1091	arg1	FGF2					1110:1113	FGF2	1110:1113	FGF2	1110:1113	The proliferation activity of the polysaccharide, YG-1, was related to Fibroblast growth factor 2 (FGF2).
32810535	2	5	from	cartilage	319:327	arg1	polysaccharides					294:308	polysaccharides	294:308	polysaccharides from the cartilage of the sturgeon, Acipenser schrenckii,	294:366	To explore glycosaminoglycans from marine fish, polysaccharides from the cartilage of the sturgeon, Acipenser schrenckii, were extracted.
32810535	2	6	theme	sturgeon	336:343	arg1	cartilage					319:327	the cartilage	315:327	the cartilage of the sturgeon, Acipenser schrenckii,	315:366	To explore glycosaminoglycans from marine fish, polysaccharides from the cartilage of the sturgeon, Acipenser schrenckii, were extracted.
32810535	8	7	theme	binding	1148:1154	arg1	4S					1124:1125	GalNAc 4S	1117:1125	GalNAc 4S of YG-1	1117:1133	GalNAc 4S of YG-1 could be the binding sites of FGF2 and FGFR.
32810535	8	7	theme	binding	1148:1154	arg1	FGFR					1174:1177	FGFR	1174:1177	FGFR	1174:1177	GalNAc 4S of YG-1 could be the binding sites of FGF2 and FGFR.
32810535	8	7	theme	binding	1148:1154	arg1	FGF2					1165:1168	FGF2	1165:1168	FGF2	1165:1168	GalNAc 4S of YG-1 could be the binding sites of FGF2 and FGFR.
32810535	8	7	theme	binding	1148:1154	arg1	sites					1156:1160	the binding sites	1144:1160	the binding sites of FGF2 and FGFR	1144:1177	GalNAc 4S of YG-1 could be the binding sites of FGF2 and FGFR.
32810535	2	8	theme	marine	281:286	arg1	fish					288:291	marine fish	281:291	marine fish	281:291	To explore glycosaminoglycans from marine fish, polysaccharides from the cartilage of the sturgeon, Acipenser schrenckii, were extracted.
32810535	8	9	theme	GalNAc	1117:1122	arg1	4S					1124:1125	GalNAc 4S	1117:1125	GalNAc 4S of YG-1	1117:1133	GalNAc 4S of YG-1 could be the binding sites of FGF2 and FGFR.
32810535	8	9	theme	GalNAc	1117:1122	arg1	FGFR					1174:1177	FGFR	1174:1177	FGFR	1174:1177	GalNAc 4S of YG-1 could be the binding sites of FGF2 and FGFR.
32810535	8	9	theme	GalNAc	1117:1122	arg1	FGF2					1165:1168	FGF2	1165:1168	FGF2	1165:1168	GalNAc 4S of YG-1 could be the binding sites of FGF2 and FGFR.
32810535	8	9	theme	GalNAc	1117:1122	arg1	sites					1156:1160	the binding sites	1144:1160	the binding sites of FGF2 and FGFR	1144:1177	GalNAc 4S of YG-1 could be the binding sites of FGF2 and FGFR.
32810535	0	10	dep	schrenckii	107:116	arg1	Acipenser					97:105	the sturgeon, Acipenser schrenckii	83:116	Acipenser	97:105	Structural characterization and proliferation activity of chondroitin sulfate from the sturgeon, Acipenser schrenckii.
32810535	8	11	theme	FGFR	1174:1177	arg1	4S					1124:1125	GalNAc 4S	1117:1125	GalNAc 4S of YG-1	1117:1133	GalNAc 4S of YG-1 could be the binding sites of FGF2 and FGFR.
32810535	8	11	theme	FGFR	1174:1177	arg1	FGFR					1174:1177	FGFR	1174:1177	FGFR	1174:1177	GalNAc 4S of YG-1 could be the binding sites of FGF2 and FGFR.
32810535	8	11	theme	FGFR	1174:1177	arg1	FGF2					1165:1168	FGF2	1165:1168	FGF2	1165:1168	GalNAc 4S of YG-1 could be the binding sites of FGF2 and FGFR.
32810535	8	11	theme	FGFR	1174:1177	arg1	sites					1156:1160	the binding sites	1144:1160	the binding sites of FGF2 and FGFR	1144:1177	GalNAc 4S of YG-1 could be the binding sites of FGF2 and FGFR.
32810535	8	12	theme	FGF2	1165:1168	arg1	4S					1124:1125	GalNAc 4S	1117:1125	GalNAc 4S of YG-1	1117:1133	GalNAc 4S of YG-1 could be the binding sites of FGF2 and FGFR.
32810535	8	12	theme	FGF2	1165:1168	arg1	FGFR					1174:1177	FGFR	1174:1177	FGFR	1174:1177	GalNAc 4S of YG-1 could be the binding sites of FGF2 and FGFR.
32810535	8	12	theme	FGF2	1165:1168	arg1	FGF2					1165:1168	FGF2	1165:1168	FGF2	1165:1168	GalNAc 4S of YG-1 could be the binding sites of FGF2 and FGFR.
32810535	8	12	theme	FGF2	1165:1168	arg1	sites					1156:1160	the binding sites	1144:1160	the binding sites of FGF2 and FGFR	1144:1177	GalNAc 4S of YG-1 could be the binding sites of FGF2 and FGFR.
32810535	3	13	theme	enzyme-assisted	390:404	arg1	extraction					406:415	enzyme-assisted extraction	390:415	enzyme-assisted extraction	390:415	Using enzyme-assisted extraction and anion-exchange chromatography, an uronic acid-containing polysaccharide, YG-1, was isolated.
32810535	7	14	theme	growth	1093:1098	arg1	factor					1100:1105	Fibroblast growth factor 2	1082:1107	Fibroblast growth factor 2 (FGF2)	1082:1114	The proliferation activity of the polysaccharide, YG-1, was related to Fibroblast growth factor 2 (FGF2).
32810535	7	14	theme	growth	1093:1098	arg1	FGF2					1110:1113	FGF2	1110:1113	FGF2	1110:1113	The proliferation activity of the polysaccharide, YG-1, was related to Fibroblast growth factor 2 (FGF2).
32810535	5	15	theme	IR	801:802	arg1	spectroscopy					804:815	IR spectroscopy	801:815	IR spectroscopy	801:815	YG-1 was confirmed to be chondroitin 4-sulfate (CS) composed of →4GlcAβ1→3GalNAc4Sβ1→ and minor →4GlcAβ1→3GalNAcβ1→, which was confirmed using IR spectroscopy, disaccharide composition analysis, and NMR.
32810535	5	16	theme	→4GlcAβ1→3GalNAc4Sβ1→	722:742	arg1	→4GlcAβ1→3GalNAcβ1→					754:772	→4GlcAβ1→3GalNAc4Sβ1→ and minor →4GlcAβ1→3GalNAcβ1→	722:772	→4GlcAβ1→3GalNAc4Sβ1→ and minor →4GlcAβ1→3GalNAcβ1→	722:772	YG-1 was confirmed to be chondroitin 4-sulfate (CS) composed of →4GlcAβ1→3GalNAc4Sβ1→ and minor →4GlcAβ1→3GalNAcβ1→, which was confirmed using IR spectroscopy, disaccharide composition analysis, and NMR.
32810535	0	17	from	activity	46:53	arg1	schrenckii					107:116	the sturgeon, Acipenser schrenckii	83:116	schrenckii	107:116	Structural characterization and proliferation activity of chondroitin sulfate from the sturgeon, Acipenser schrenckii.
32810535	7	18	theme	polysaccharide	1045:1058	arg1	activity					1029:1036	The proliferation activity	1011:1036	The proliferation activity of the polysaccharide, YG-1,	1011:1065	The proliferation activity of the polysaccharide, YG-1, was related to Fibroblast growth factor 2 (FGF2).
32810535	7	18	theme	polysaccharide	1045:1058	arg1	related					1071:1077	related	1071:1077	related	1071:1077	The proliferation activity of the polysaccharide, YG-1, was related to Fibroblast growth factor 2 (FGF2).
32810535	3	19	theme	anion-exchange	421:434	arg1	chromatography					436:449	anion-exchange chromatography	421:449	anion-exchange chromatography	421:449	Using enzyme-assisted extraction and anion-exchange chromatography, an uronic acid-containing polysaccharide, YG-1, was isolated.
32810535	0	20	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and proliferation activity of chondroitin sulfate from the sturgeon, Acipenser schrenckii.
32810535	0	21	theme	proliferation	32:44	arg1	activity					46:53	proliferation activity	32:53	proliferation activity	32:53	Structural characterization and proliferation activity of chondroitin sulfate from the sturgeon, Acipenser schrenckii.
32810535	6	22	theme	scratch-wound	907:919	arg1	assays					921:926	scratch-wound assays	907:926	scratch-wound assays	907:926	Bioactivity studies, including MTT assay and scratch-wound assays revealed that CS from Acipenser schrenckii had significant proliferation activity.
32810535	1	23	theme	important	183:191	arg1	resources					193:201	important resources	183:201	important resources of the bioactive chondroitin sulfate (CS)	183:243	The cartilages of marine fish, such as sharks and sturgeon, are important resources of the bioactive chondroitin sulfate (CS).
32810535	1	23	theme	important	183:191	arg1	cartilages					123:132	The cartilages	119:132	The cartilages	119:132	The cartilages of marine fish, such as sharks and sturgeon, are important resources of the bioactive chondroitin sulfate (CS).
32810535	1	23	theme	important	183:191	arg1	sturgeon					169:176	sturgeon	169:176	sturgeon	169:176	The cartilages of marine fish, such as sharks and sturgeon, are important resources of the bioactive chondroitin sulfate (CS).
32810535	1	23	theme	important	183:191	arg1	sharks					158:163	sharks	158:163	sharks	158:163	The cartilages of marine fish, such as sharks and sturgeon, are important resources of the bioactive chondroitin sulfate (CS).
32810535	8	24	theme	YG-1	1130:1133	arg1	4S					1124:1125	GalNAc 4S	1117:1125	GalNAc 4S of YG-1	1117:1133	GalNAc 4S of YG-1 could be the binding sites of FGF2 and FGFR.
32810535	8	24	theme	YG-1	1130:1133	arg1	FGFR					1174:1177	FGFR	1174:1177	FGFR	1174:1177	GalNAc 4S of YG-1 could be the binding sites of FGF2 and FGFR.
32810535	8	24	theme	YG-1	1130:1133	arg1	FGF2					1165:1168	FGF2	1165:1168	FGF2	1165:1168	GalNAc 4S of YG-1 could be the binding sites of FGF2 and FGFR.
32810535	8	24	theme	YG-1	1130:1133	arg1	sites					1156:1160	the binding sites	1144:1160	the binding sites of FGF2 and FGFR	1144:1177	GalNAc 4S of YG-1 could be the binding sites of FGF2 and FGFR.
32810535	6	25	contain	had	971:973	arg1	CS					942:943	CS	942:943	CS from Acipenser schrenckii	942:969	Bioactivity studies, including MTT assay and scratch-wound assays revealed that CS from Acipenser schrenckii had significant proliferation activity.
32810535	6	25	contain	had	971:973	arg2	activity					1001:1008	significant proliferation activity	975:1008	significant proliferation activity	975:1008	Bioactivity studies, including MTT assay and scratch-wound assays revealed that CS from Acipenser schrenckii had significant proliferation activity.
32810535	7	26	theme	proliferation	1015:1027	arg1	activity					1029:1036	The proliferation activity	1011:1036	The proliferation activity of the polysaccharide, YG-1,	1011:1065	The proliferation activity of the polysaccharide, YG-1, was related to Fibroblast growth factor 2 (FGF2).
32810535	7	26	theme	proliferation	1015:1027	arg1	related					1071:1077	related	1071:1077	related	1071:1077	The proliferation activity of the polysaccharide, YG-1, was related to Fibroblast growth factor 2 (FGF2).
32810535	3	27	theme	uronic	455:460	arg1	YG-1					494:497	YG-1	494:497	YG-1	494:497	Using enzyme-assisted extraction and anion-exchange chromatography, an uronic acid-containing polysaccharide, YG-1, was isolated.
32810535	3	27	theme	uronic	455:460	arg1	polysaccharide					478:491	an uronic acid-containing polysaccharide	452:491	an uronic acid-containing polysaccharide	452:491	Using enzyme-assisted extraction and anion-exchange chromatography, an uronic acid-containing polysaccharide, YG-1, was isolated.
32810535	6	28	theme	significant	975:985	arg1	activity					1001:1008	significant proliferation activity	975:1008	significant proliferation activity	975:1008	Bioactivity studies, including MTT assay and scratch-wound assays revealed that CS from Acipenser schrenckii had significant proliferation activity.
32810535	6	29	theme	MTT	893:895	arg1	assay					897:901	MTT assay	893:901	MTT assay	893:901	Bioactivity studies, including MTT assay and scratch-wound assays revealed that CS from Acipenser schrenckii had significant proliferation activity.
32810535	5	30	theme	minor	748:752	arg1	→4GlcAβ1→3GalNAcβ1→					754:772	→4GlcAβ1→3GalNAc4Sβ1→ and minor →4GlcAβ1→3GalNAcβ1→	722:772	→4GlcAβ1→3GalNAc4Sβ1→ and minor →4GlcAβ1→3GalNAcβ1→	722:772	YG-1 was confirmed to be chondroitin 4-sulfate (CS) composed of →4GlcAβ1→3GalNAc4Sβ1→ and minor →4GlcAβ1→3GalNAcβ1→, which was confirmed using IR spectroscopy, disaccharide composition analysis, and NMR.
32810535	3	31	theme	acid-containing	462:476	arg1	YG-1					494:497	YG-1	494:497	YG-1	494:497	Using enzyme-assisted extraction and anion-exchange chromatography, an uronic acid-containing polysaccharide, YG-1, was isolated.
32810535	3	31	theme	acid-containing	462:476	arg1	polysaccharide					478:491	an uronic acid-containing polysaccharide	452:491	an uronic acid-containing polysaccharide	452:491	Using enzyme-assisted extraction and anion-exchange chromatography, an uronic acid-containing polysaccharide, YG-1, was isolated.
32810535	0	32	theme	sulfate	70:76	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and proliferation activity of chondroitin sulfate from the sturgeon, Acipenser schrenckii.
32810535	0	32	theme	sulfate	70:76	arg1	activity					46:53	proliferation activity	32:53	proliferation activity	32:53	Structural characterization and proliferation activity of chondroitin sulfate from the sturgeon, Acipenser schrenckii.
32810535	6	33	theme	proliferation	987:999	arg1	activity					1001:1008	significant proliferation activity	975:1008	significant proliferation activity	975:1008	Bioactivity studies, including MTT assay and scratch-wound assays revealed that CS from Acipenser schrenckii had significant proliferation activity.
32810535	0	34	theme	chondroitin	58:68	arg1	sulfate					70:76	chondroitin sulfate	58:76	chondroitin sulfate from the sturgeon, Acipenser schrenckii	58:116	Structural characterization and proliferation activity of chondroitin sulfate from the sturgeon, Acipenser schrenckii.
32810535	4	35	theme	molecular	606:614	arg1	weight					616:621	its molecular weight	602:621	its molecular weight	602:621	YG-1 is composed of GlcN, GlcUA, GalN, and Gal, in the ratio of 1.4: 3.4: 3.7: 1.0, and its molecular weight was determined to be 3.0 × 105 Da.
32810535	1	36	theme	marine	137:142	arg1	fish					144:147	marine fish	137:147	marine fish	137:147	The cartilages of marine fish, such as sharks and sturgeon, are important resources of the bioactive chondroitin sulfate (CS).
32810535	1	37	theme	bioactive	210:218	arg1	sulfate					232:238	the bioactive chondroitin sulfate	206:238	the bioactive chondroitin sulfate (CS)	206:243	The cartilages of marine fish, such as sharks and sturgeon, are important resources of the bioactive chondroitin sulfate (CS).
32810535	1	37	theme	bioactive	210:218	arg1	CS					241:242	CS	241:242	CS	241:242	The cartilages of marine fish, such as sharks and sturgeon, are important resources of the bioactive chondroitin sulfate (CS).
32810535	0	38	from	schrenckii	107:116	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and proliferation activity of chondroitin sulfate from the sturgeon, Acipenser schrenckii.
32810535	0	38	from	schrenckii	107:116	arg1	activity					46:53	proliferation activity	32:53	proliferation activity	32:53	Structural characterization and proliferation activity of chondroitin sulfate from the sturgeon, Acipenser schrenckii.
32810535	0	38	from	schrenckii	107:116	arg1	sulfate					70:76	chondroitin sulfate	58:76	chondroitin sulfate from the sturgeon, Acipenser schrenckii	58:116	Structural characterization and proliferation activity of chondroitin sulfate from the sturgeon, Acipenser schrenckii.
32810535	1	39	theme	fish	144:147	arg1	resources					193:201	important resources	183:201	important resources of the bioactive chondroitin sulfate (CS)	183:243	The cartilages of marine fish, such as sharks and sturgeon, are important resources of the bioactive chondroitin sulfate (CS).
32810535	1	39	theme	fish	144:147	arg1	cartilages					123:132	The cartilages	119:132	The cartilages	119:132	The cartilages of marine fish, such as sharks and sturgeon, are important resources of the bioactive chondroitin sulfate (CS).
32810535	1	39	theme	fish	144:147	arg1	sturgeon					169:176	sturgeon	169:176	sturgeon	169:176	The cartilages of marine fish, such as sharks and sturgeon, are important resources of the bioactive chondroitin sulfate (CS).
32810535	1	39	theme	fish	144:147	arg1	sharks					158:163	sharks	158:163	sharks	158:163	The cartilages of marine fish, such as sharks and sturgeon, are important resources of the bioactive chondroitin sulfate (CS).
32810535	1	40	theme	chondroitin	220:230	arg1	sulfate					232:238	the bioactive chondroitin sulfate	206:238	the bioactive chondroitin sulfate (CS)	206:243	The cartilages of marine fish, such as sharks and sturgeon, are important resources of the bioactive chondroitin sulfate (CS).
32810535	1	40	theme	chondroitin	220:230	arg1	CS					241:242	CS	241:242	CS	241:242	The cartilages of marine fish, such as sharks and sturgeon, are important resources of the bioactive chondroitin sulfate (CS).
32810535	5	41	theme	composition	831:841	arg1	analysis					843:850	disaccharide composition analysis	818:850	disaccharide composition analysis	818:850	YG-1 was confirmed to be chondroitin 4-sulfate (CS) composed of →4GlcAβ1→3GalNAc4Sβ1→ and minor →4GlcAβ1→3GalNAcβ1→, which was confirmed using IR spectroscopy, disaccharide composition analysis, and NMR.
32810535	5	42	theme	disaccharide	818:829	arg1	analysis					843:850	disaccharide composition analysis	818:850	disaccharide composition analysis	818:850	YG-1 was confirmed to be chondroitin 4-sulfate (CS) composed of →4GlcAβ1→3GalNAc4Sβ1→ and minor →4GlcAβ1→3GalNAcβ1→, which was confirmed using IR spectroscopy, disaccharide composition analysis, and NMR.
32810535	1	43	theme	sulfate	232:238	arg1	resources					193:201	important resources	183:201	important resources of the bioactive chondroitin sulfate (CS)	183:243	The cartilages of marine fish, such as sharks and sturgeon, are important resources of the bioactive chondroitin sulfate (CS).
32810535	1	43	theme	sulfate	232:238	arg1	cartilages					123:132	The cartilages	119:132	The cartilages	119:132	The cartilages of marine fish, such as sharks and sturgeon, are important resources of the bioactive chondroitin sulfate (CS).
32810535	1	43	theme	sulfate	232:238	arg1	sturgeon					169:176	sturgeon	169:176	sturgeon	169:176	The cartilages of marine fish, such as sharks and sturgeon, are important resources of the bioactive chondroitin sulfate (CS).
32810535	1	43	theme	sulfate	232:238	arg1	sharks					158:163	sharks	158:163	sharks	158:163	The cartilages of marine fish, such as sharks and sturgeon, are important resources of the bioactive chondroitin sulfate (CS).
32810535	5	44	theme	chondroitin	683:693	arg1	CS					706:707	CS	706:707	CS	706:707	YG-1 was confirmed to be chondroitin 4-sulfate (CS) composed of →4GlcAβ1→3GalNAc4Sβ1→ and minor →4GlcAβ1→3GalNAcβ1→, which was confirmed using IR spectroscopy, disaccharide composition analysis, and NMR.
32810535	5	44	theme	chondroitin	683:693	arg1	4-sulfate					695:703	chondroitin 4-sulfate	683:703	chondroitin 4-sulfate (CS) composed of →4GlcAβ1→3GalNAc4Sβ1→ and minor →4GlcAβ1→3GalNAcβ1→, which was confirmed using IR spectroscopy, disaccharide composition analysis, and NMR	683:859	YG-1 was confirmed to be chondroitin 4-sulfate (CS) composed of →4GlcAβ1→3GalNAc4Sβ1→ and minor →4GlcAβ1→3GalNAcβ1→, which was confirmed using IR spectroscopy, disaccharide composition analysis, and NMR.
32810535	6	45	theme	Bioactivity	862:872	arg1	studies					874:880	Bioactivity studies	862:880	Bioactivity studies	862:880	Bioactivity studies, including MTT assay and scratch-wound assays revealed that CS from Acipenser schrenckii had significant proliferation activity.
32810535	6	45	theme	Bioactivity	862:872	arg1	assay					897:901	MTT assay	893:901	MTT assay	893:901	Bioactivity studies, including MTT assay and scratch-wound assays revealed that CS from Acipenser schrenckii had significant proliferation activity.
32810535	6	45	theme	Bioactivity	862:872	arg1	assays					921:926	scratch-wound assays	907:926	scratch-wound assays	907:926	Bioactivity studies, including MTT assay and scratch-wound assays revealed that CS from Acipenser schrenckii had significant proliferation activity.
32810535	6	46	theme	Acipenser	950:958	arg1	schrenckii					960:969	Acipenser schrenckii	950:969	Acipenser schrenckii	950:969	Bioactivity studies, including MTT assay and scratch-wound assays revealed that CS from Acipenser schrenckii had significant proliferation activity.
32810535	2	47	theme	Acipenser	346:354	arg1	sturgeon					336:343	the sturgeon	332:343	the sturgeon	332:343	To explore glycosaminoglycans from marine fish, polysaccharides from the cartilage of the sturgeon, Acipenser schrenckii, were extracted.
32810535	2	47	theme	Acipenser	346:354	arg1	schrenckii					356:365	Acipenser schrenckii	346:365	Acipenser schrenckii	346:365	To explore glycosaminoglycans from marine fish, polysaccharides from the cartilage of the sturgeon, Acipenser schrenckii, were extracted.
32810535	4	48	theme	1.4	578:580	arg1	3.0 × 105 Da					644:655	3.0 × 105 Da	644:655	3.0 × 105 Da	644:655	YG-1 is composed of GlcN, GlcUA, GalN, and Gal, in the ratio of 1.4: 3.4: 3.7: 1.0, and its molecular weight was determined to be 3.0 × 105 Da.
32810535	4	48	theme	1.4	578:580	arg1	weight					616:621	its molecular weight	602:621	its molecular weight	602:621	YG-1 is composed of GlcN, GlcUA, GalN, and Gal, in the ratio of 1.4: 3.4: 3.7: 1.0, and its molecular weight was determined to be 3.0 × 105 Da.
32810535	4	48	theme	1.4	578:580	arg1	ratio					569:573	the ratio	565:573	the ratio of 1.4	565:580	YG-1 is composed of GlcN, GlcUA, GalN, and Gal, in the ratio of 1.4: 3.4: 3.7: 1.0, and its molecular weight was determined to be 3.0 × 105 Da.
34051599	0	0	theme	camel	90:94	arg1	powder					101:106	spray-dried camel milk powder	78:106	spray-dried camel milk powder	78:106	Changes in surface chemical composition relating to rehydration properties of spray-dried camel milk powder during accelerated storage.
34051599	4	1	theme	poor	771:774	arg1	wettability					776:786	very poor wettability	766:786	very poor wettability	766:786	Although fresh camel milk powder had very poor wettability, it displayed very high dispersibility and solubility (99%).
34051599	0	2	theme	spray-dried	78:88	arg1	powder					101:106	spray-dried camel milk powder	78:106	spray-dried camel milk powder	78:106	Changes in surface chemical composition relating to rehydration properties of spray-dried camel milk powder during accelerated storage.
34051599	0	3	theme	powder	101:106	arg1	properties					64:73	rehydration properties	52:73	rehydration properties of spray-dried camel milk powder	52:106	Changes in surface chemical composition relating to rehydration properties of spray-dried camel milk powder during accelerated storage.
34051599	0	4	from	Changes	0:6	arg1	composition					28:38	surface chemical composition	11:38	surface chemical composition relating to rehydration properties of spray-dried camel milk powder during accelerated storage	11:133	Changes in surface chemical composition relating to rehydration properties of spray-dried camel milk powder during accelerated storage.
34051599	0	5	theme	milk	96:99	arg1	powder					101:106	spray-dried camel milk powder	78:106	spray-dried camel milk powder	78:106	Changes in surface chemical composition relating to rehydration properties of spray-dried camel milk powder during accelerated storage.
34051599	6	6	theme	period	1054:1059	arg1	end					1035:1037	the end	1031:1037	the end of the storage period	1031:1059	However, at the end of the storage period, camel milk powder still retained very high solubility (>93%).
34051599	0	7	theme	accelerated	115:125	arg1	storage					127:133	accelerated storage	115:133	accelerated storage	115:133	Changes in surface chemical composition relating to rehydration properties of spray-dried camel milk powder during accelerated storage.
34051599	3	8	theme	slight	655:660	arg1	agglomeration					662:674	slight agglomeration	655:674	slight agglomeration of the powder	655:688	During storage, the surface protein and lactose content decreased while the surface lipid content increased, resulting in an increase in surface hydrophobicity and slight agglomeration of the powder, especially for powder kept at 33% RH.
34051599	3	9	from	agglomeration	662:674	arg1	hydrophobicity					636:649	surface hydrophobicity	628:649	surface hydrophobicity	628:649	During storage, the surface protein and lactose content decreased while the surface lipid content increased, resulting in an increase in surface hydrophobicity and slight agglomeration of the powder, especially for powder kept at 33% RH.
34051599	6	10	theme	storage	1046:1052	arg1	period					1054:1059	the storage period	1042:1059	the storage period	1042:1059	However, at the end of the storage period, camel milk powder still retained very high solubility (>93%).
34051599	5	11	from	increase	984:991	arg1	content					1010:1016	surface lipid content	996:1016	surface lipid content	996:1016	During storage, dispersibility and solubility declined with increasing storage time and increasing RH levels, which correlated with an increase in surface lipid content.
34051599	2	12	theme	powder	400:405	arg1	surface					356:362	the surface	352:362	the surface of the fresh spray-dried camel milk powder (t = 0)	352:413	The results showed that the surface of the fresh spray-dried camel milk powder (t = 0) was dominated by lipids (78%), followed by proteins (16%) and lactose (6%).
34051599	3	13	theme	surface	628:634	arg1	hydrophobicity					636:649	surface hydrophobicity	628:649	surface hydrophobicity	628:649	During storage, the surface protein and lactose content decreased while the surface lipid content increased, resulting in an increase in surface hydrophobicity and slight agglomeration of the powder, especially for powder kept at 33% RH.
34051599	2	14	dep	dominated	419:427	arg1	followed					446:453	followed	446:453	followed by proteins (16%) and lactose (6%)	446:488	The results showed that the surface of the fresh spray-dried camel milk powder (t = 0) was dominated by lipids (78%), followed by proteins (16%) and lactose (6%).
34051599	1	15	from	Alterations	136:146	arg1	composition					168:178	surface chemical composition	151:178	surface chemical composition relating to rehydration properties of spray-dried camel milk powders during accelerated storage (11-33% RH, 37 °C) over 18 weeks	151:307	Alterations in surface chemical composition relating to rehydration properties of spray-dried camel milk powders during accelerated storage (11-33% RH, 37 °C) over 18 weeks were investigated.
34051599	3	16	theme	%	723:723	arg1	RH					725:726	33% RH	721:726	33% RH	721:726	During storage, the surface protein and lactose content decreased while the surface lipid content increased, resulting in an increase in surface hydrophobicity and slight agglomeration of the powder, especially for powder kept at 33% RH.
34051599	1	17	theme	rehydration	192:202	arg1	properties					204:213	rehydration properties	192:213	rehydration properties of spray-dried camel milk powders during accelerated storage (11-33% RH, 37 °C) over 18 weeks	192:307	Alterations in surface chemical composition relating to rehydration properties of spray-dried camel milk powders during accelerated storage (11-33% RH, 37 °C) over 18 weeks were investigated.
34051599	5	18	theme	lipid	1004:1008	arg1	content					1010:1016	surface lipid content	996:1016	surface lipid content	996:1016	During storage, dispersibility and solubility declined with increasing storage time and increasing RH levels, which correlated with an increase in surface lipid content.
34051599	2	19	theme	camel	389:393	arg1	t = 0					408:412	t = 0	408:412	t = 0	408:412	The results showed that the surface of the fresh spray-dried camel milk powder (t = 0) was dominated by lipids (78%), followed by proteins (16%) and lactose (6%).
34051599	2	19	theme	camel	389:393	arg1	powder					400:405	the fresh spray-dried camel milk powder	367:405	the fresh spray-dried camel milk powder (t = 0)	367:413	The results showed that the surface of the fresh spray-dried camel milk powder (t = 0) was dominated by lipids (78%), followed by proteins (16%) and lactose (6%).
34051599	4	20	theme	milk	750:753	arg1	powder					755:760	fresh camel milk powder	738:760	fresh camel milk powder	738:760	Although fresh camel milk powder had very poor wettability, it displayed very high dispersibility and solubility (99%).
34051599	2	21	theme	spray-dried	377:387	arg1	t = 0					408:412	t = 0	408:412	t = 0	408:412	The results showed that the surface of the fresh spray-dried camel milk powder (t = 0) was dominated by lipids (78%), followed by proteins (16%) and lactose (6%).
34051599	2	21	theme	spray-dried	377:387	arg1	powder					400:405	the fresh spray-dried camel milk powder	367:405	the fresh spray-dried camel milk powder (t = 0)	367:413	The results showed that the surface of the fresh spray-dried camel milk powder (t = 0) was dominated by lipids (78%), followed by proteins (16%) and lactose (6%).
34051599	0	22	theme	chemical	19:26	arg1	composition					28:38	surface chemical composition	11:38	surface chemical composition relating to rehydration properties of spray-dried camel milk powder during accelerated storage	11:133	Changes in surface chemical composition relating to rehydration properties of spray-dried camel milk powder during accelerated storage.
34051599	3	23	theme	powder	683:688	arg1	increase					616:623	an increase	613:623	an increase in surface hydrophobicity	613:649	During storage, the surface protein and lactose content decreased while the surface lipid content increased, resulting in an increase in surface hydrophobicity and slight agglomeration of the powder, especially for powder kept at 33% RH.
34051599	3	23	theme	powder	683:688	arg1	agglomeration					662:674	slight agglomeration	655:674	slight agglomeration of the powder	655:688	During storage, the surface protein and lactose content decreased while the surface lipid content increased, resulting in an increase in surface hydrophobicity and slight agglomeration of the powder, especially for powder kept at 33% RH.
34051599	1	24	theme	spray-dried	218:228	arg1	powders					241:247	spray-dried camel milk powders	218:247	spray-dried camel milk powders during accelerated storage (11-33% RH, 37 °C) over 18 weeks	218:307	Alterations in surface chemical composition relating to rehydration properties of spray-dried camel milk powders during accelerated storage (11-33% RH, 37 °C) over 18 weeks were investigated.
34051599	0	25	theme	surface	11:17	arg1	composition					28:38	surface chemical composition	11:38	surface chemical composition relating to rehydration properties of spray-dried camel milk powder during accelerated storage	11:133	Changes in surface chemical composition relating to rehydration properties of spray-dried camel milk powder during accelerated storage.
34051599	2	26	theme	fresh	371:375	arg1	t = 0					408:412	t = 0	408:412	t = 0	408:412	The results showed that the surface of the fresh spray-dried camel milk powder (t = 0) was dominated by lipids (78%), followed by proteins (16%) and lactose (6%).
34051599	2	26	theme	fresh	371:375	arg1	powder					400:405	the fresh spray-dried camel milk powder	367:405	the fresh spray-dried camel milk powder (t = 0)	367:413	The results showed that the surface of the fresh spray-dried camel milk powder (t = 0) was dominated by lipids (78%), followed by proteins (16%) and lactose (6%).
34051599	3	27	theme	surface	567:573	arg1	content					581:587	the surface lipid content	563:587	the surface lipid content	563:587	During storage, the surface protein and lactose content decreased while the surface lipid content increased, resulting in an increase in surface hydrophobicity and slight agglomeration of the powder, especially for powder kept at 33% RH.
34051599	1	28	theme	camel	230:234	arg1	powders					241:247	spray-dried camel milk powders	218:247	spray-dried camel milk powders during accelerated storage (11-33% RH, 37 °C) over 18 weeks	218:307	Alterations in surface chemical composition relating to rehydration properties of spray-dried camel milk powders during accelerated storage (11-33% RH, 37 °C) over 18 weeks were investigated.
34051599	3	29	theme	lipid	575:579	arg1	content					581:587	the surface lipid content	563:587	the surface lipid content	563:587	During storage, the surface protein and lactose content decreased while the surface lipid content increased, resulting in an increase in surface hydrophobicity and slight agglomeration of the powder, especially for powder kept at 33% RH.
34051599	3	30	theme	surface	511:517	arg1	protein					519:525	the surface protein	507:525	the surface protein	507:525	During storage, the surface protein and lactose content decreased while the surface lipid content increased, resulting in an increase in surface hydrophobicity and slight agglomeration of the powder, especially for powder kept at 33% RH.
34051599	1	31	theme	milk	236:239	arg1	powders					241:247	spray-dried camel milk powders	218:247	spray-dried camel milk powders during accelerated storage (11-33% RH, 37 °C) over 18 weeks	218:307	Alterations in surface chemical composition relating to rehydration properties of spray-dried camel milk powders during accelerated storage (11-33% RH, 37 °C) over 18 weeks were investigated.
34051599	5	32	theme	storage	920:926	arg1	time					928:931	storage time	920:931	storage time	920:931	During storage, dispersibility and solubility declined with increasing storage time and increasing RH levels, which correlated with an increase in surface lipid content.
34051599	1	33	theme	powders	241:247	arg1	properties					204:213	rehydration properties	192:213	rehydration properties of spray-dried camel milk powders during accelerated storage (11-33% RH, 37 °C) over 18 weeks	192:307	Alterations in surface chemical composition relating to rehydration properties of spray-dried camel milk powders during accelerated storage (11-33% RH, 37 °C) over 18 weeks were investigated.
34051599	0	34	theme	rehydration	52:62	arg1	properties					64:73	rehydration properties	52:73	rehydration properties of spray-dried camel milk powder	52:106	Changes in surface chemical composition relating to rehydration properties of spray-dried camel milk powder during accelerated storage.
34051599	4	35	theme	fresh	738:742	arg1	powder					755:760	fresh camel milk powder	738:760	fresh camel milk powder	738:760	Although fresh camel milk powder had very poor wettability, it displayed very high dispersibility and solubility (99%).
34051599	5	36	theme	surface	996:1002	arg1	content					1010:1016	surface lipid content	996:1016	surface lipid content	996:1016	During storage, dispersibility and solubility declined with increasing storage time and increasing RH levels, which correlated with an increase in surface lipid content.
34051599	4	37	theme	camel	744:748	arg1	powder					755:760	fresh camel milk powder	738:760	fresh camel milk powder	738:760	Although fresh camel milk powder had very poor wettability, it displayed very high dispersibility and solubility (99%).
34051599	6	38	theme	camel	1062:1066	arg1	powder					1073:1078	camel milk powder	1062:1078	camel milk powder	1062:1078	However, at the end of the storage period, camel milk powder still retained very high solubility (>93%).
34051599	4	39	theme	high	807:810	arg1	%					845:845	99%	843:845	99%	843:845	Although fresh camel milk powder had very poor wettability, it displayed very high dispersibility and solubility (99%).
34051599	4	39	theme	high	807:810	arg1	dispersibility					812:825	very high dispersibility	802:825	very high dispersibility	802:825	Although fresh camel milk powder had very poor wettability, it displayed very high dispersibility and solubility (99%).
34051599	5	40	theme	RH	948:949	arg1	levels					951:956	RH levels	948:956	RH levels	948:956	During storage, dispersibility and solubility declined with increasing storage time and increasing RH levels, which correlated with an increase in surface lipid content.
34051599	6	41	theme	high	1100:1103	arg1	%					1120:1120	>93%	1117:1120	>93%	1117:1120	However, at the end of the storage period, camel milk powder still retained very high solubility (>93%).
34051599	6	41	theme	high	1100:1103	arg1	solubility					1105:1114	very high solubility	1095:1114	very high solubility (>93%)	1095:1121	However, at the end of the storage period, camel milk powder still retained very high solubility (>93%).
34051599	4	42	contain	had	762:764	arg1	powder					755:760	fresh camel milk powder	738:760	fresh camel milk powder	738:760	Although fresh camel milk powder had very poor wettability, it displayed very high dispersibility and solubility (99%).
34051599	4	42	contain	had	762:764	arg2	wettability					776:786	very poor wettability	766:786	very poor wettability	766:786	Although fresh camel milk powder had very poor wettability, it displayed very high dispersibility and solubility (99%).
34051599	3	43	theme	33	721:722	arg1	%					723:723	%	723:723	%	723:723	During storage, the surface protein and lactose content decreased while the surface lipid content increased, resulting in an increase in surface hydrophobicity and slight agglomeration of the powder, especially for powder kept at 33% RH.
34051599	1	44	theme	accelerated	256:266	arg1	storage					268:274	accelerated storage	256:274	accelerated storage (11-33% RH, 37 °C) over 18 weeks	256:307	Alterations in surface chemical composition relating to rehydration properties of spray-dried camel milk powders during accelerated storage (11-33% RH, 37 °C) over 18 weeks were investigated.
34051599	1	44	theme	accelerated	256:266	arg1	%					282:282	11-33% RH	277:285	11-33% RH	277:285	Alterations in surface chemical composition relating to rehydration properties of spray-dried camel milk powders during accelerated storage (11-33% RH, 37 °C) over 18 weeks were investigated.
34051599	1	45	dep	%	282:282	arg1	37 °C					288:292	37 °C	288:292	37 °C	288:292	Alterations in surface chemical composition relating to rehydration properties of spray-dried camel milk powders during accelerated storage (11-33% RH, 37 °C) over 18 weeks were investigated.
34051599	6	46	theme	milk	1068:1071	arg1	powder					1073:1078	camel milk powder	1062:1078	camel milk powder	1062:1078	However, at the end of the storage period, camel milk powder still retained very high solubility (>93%).
34051599	1	47	theme	surface	151:157	arg1	composition					168:178	surface chemical composition	151:178	surface chemical composition relating to rehydration properties of spray-dried camel milk powders during accelerated storage (11-33% RH, 37 °C) over 18 weeks	151:307	Alterations in surface chemical composition relating to rehydration properties of spray-dried camel milk powders during accelerated storage (11-33% RH, 37 °C) over 18 weeks were investigated.
34051599	2	48	theme	milk	395:398	arg1	t = 0					408:412	t = 0	408:412	t = 0	408:412	The results showed that the surface of the fresh spray-dried camel milk powder (t = 0) was dominated by lipids (78%), followed by proteins (16%) and lactose (6%).
34051599	2	48	theme	milk	395:398	arg1	powder					400:405	the fresh spray-dried camel milk powder	367:405	the fresh spray-dried camel milk powder (t = 0)	367:413	The results showed that the surface of the fresh spray-dried camel milk powder (t = 0) was dominated by lipids (78%), followed by proteins (16%) and lactose (6%).
34051599	3	49	from	increase	616:623	arg1	hydrophobicity					636:649	surface hydrophobicity	628:649	surface hydrophobicity	628:649	During storage, the surface protein and lactose content decreased while the surface lipid content increased, resulting in an increase in surface hydrophobicity and slight agglomeration of the powder, especially for powder kept at 33% RH.
34051599	3	50	theme	lactose	531:537	arg1	content					539:545	lactose content	531:545	lactose content	531:545	During storage, the surface protein and lactose content decreased while the surface lipid content increased, resulting in an increase in surface hydrophobicity and slight agglomeration of the powder, especially for powder kept at 33% RH.
34051599	1	51	theme	chemical	159:166	arg1	composition					168:178	surface chemical composition	151:178	surface chemical composition relating to rehydration properties of spray-dried camel milk powders during accelerated storage (11-33% RH, 37 °C) over 18 weeks	151:307	Alterations in surface chemical composition relating to rehydration properties of spray-dried camel milk powders during accelerated storage (11-33% RH, 37 °C) over 18 weeks were investigated.
34661088	6	0	theme	therapeutic	1126:1136	arg1	development					1138:1148	SARS-CoV-2 and therapeutic development	1111:1148	SARS-CoV-2 and therapeutic development of Covid-19, particularly using recombinant S1 proteins as models	1111:1214	The results should contribute to future pathological studies of SARS-CoV-2 and therapeutic development of Covid-19, particularly using recombinant S1 proteins as models.
34661088	5	1	with	interaction	1024:1034	arg1	ACE2					1041:1044	ACE2	1041:1044	ACE2	1041:1044	Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2.
34661088	4	2	theme	S1	673:674	arg1	proteins					676:683	de-N-glycosylated S1 proteins	655:683	de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells	655:839	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	1	3	theme	spike	142:146	arg1	protein					152:158	The densely glycosylated spike (S) protein	117:158	The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface	117:245	The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2).
34661088	1	4	theme	SARS-CoV-2	227:236	arg1	surface					239:245	severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface	178:245	severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface	178:245	The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2).
34661088	0	5	theme	virus	65:69	arg1	interaction					71:81	the virus interaction	61:81	the virus interaction with the host cell ACE2 receptor	61:114	The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor.
34661088	0	6	from	effect	4:9	arg1	interaction					71:81	the virus interaction	61:81	the virus interaction with the host cell ACE2 receptor	61:114	The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor.
34661088	0	7	with	interaction	71:81	arg1	receptor					107:114	the host cell ACE2 receptor	88:114	the host cell ACE2 receptor	88:114	The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor.
34661088	3	8	theme	N-glycosylation	460:474	arg1	effect					440:445	the effect	436:445	the effect of different N-glycosylation of S1 protein on its binding to ACE2	436:511	In this study, we investigated the effect of different N-glycosylation of S1 protein on its binding to ACE2.
34661088	4	9	theme	real-time	520:528	arg1	resonance					546:554	real-time surface plasmon resonance	520:554	real-time surface plasmon resonance assay	520:560	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	4	10	dep	demonstrated	588:599	arg1	Using					514:518	Using	514:518	Using real-time surface plasmon resonance assay	514:560	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	5	11	theme	protein-ACE2	882:893	arg1	complex					904:910	the S1 protein-ACE2 receptor complex	875:910	the S1 protein-ACE2 receptor complex	875:910	Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2.
34661088	4	12	theme	resonance	546:554	arg1	assay					556:560	real-time surface plasmon resonance assay	520:560	real-time surface plasmon resonance assay	520:560	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	4	13	theme	expression	715:724	arg1	systems					726:732	three different expression systems	699:732	three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells	699:839	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	4	13	theme	expression	715:724	arg1	ovarian					780:786	ovarian	780:786	ovarian	780:786	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	4	13	theme	expression	715:724	arg1	variants					796:803	two variants	792:803	two variants of human embryonic kidney 293 cells	792:839	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	4	13	theme	expression	715:724	arg1	baculovirus-insect					744:761	baculovirus-insect	744:761	baculovirus-insect	744:761	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	5	14	theme	different	977:985	arg1	types					987:991	different types	977:991	different types of N-glycans	977:1004	Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2.
34661088	5	15	theme	hindrance	932:940	arg1	effects					966:972	the steric hindrance and Coulombic repulsion effects	921:972	the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2	921:1044	Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2.
34661088	3	16	theme	different	450:458	arg1	N-glycosylation					460:474	different N-glycosylation	450:474	different N-glycosylation of S1 protein	450:488	In this study, we investigated the effect of different N-glycosylation of S1 protein on its binding to ACE2.
34661088	4	17	theme	plasmon	538:544	arg1	resonance					546:554	real-time surface plasmon resonance	520:554	real-time surface plasmon resonance assay	520:560	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	0	18	theme	cell	97:100	arg1	receptor					107:114	the host cell ACE2 receptor	88:114	the host cell ACE2 receptor	88:114	The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor.
34661088	4	19	theme	cells	835:839	arg1	variants					796:803	two variants	792:803	two variants of human embryonic kidney 293 cells	792:839	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	3	20	gly	N-glycosylation	460:474	arg1	protein					482:488	S1 protein	479:488	S1 protein	479:488	In this study, we investigated the effect of different N-glycosylation of S1 protein on its binding to ACE2.
34661088	5	21	theme	dynamic	852:858	arg1	simulations					860:870	Molecular dynamic simulations	842:870	Molecular dynamic simulations of the S1 protein-ACE2 receptor complex	842:910	Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2.
34661088	0	22	theme	host	92:95	arg1	receptor					107:114	the host cell ACE2 receptor	88:114	the host cell ACE2 receptor	88:114	The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor.
34661088	5	23	theme	complex	904:910	arg1	simulations					860:870	Molecular dynamic simulations	842:870	Molecular dynamic simulations of the S1 protein-ACE2 receptor complex	842:910	Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2.
34661088	4	24	theme	surface	530:536	arg1	resonance					546:554	real-time surface plasmon resonance	520:554	real-time surface plasmon resonance assay	520:560	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	5	25	theme	receptor	895:902	arg1	complex					904:910	the S1 protein-ACE2 receptor complex	875:910	the S1 protein-ACE2 receptor complex	875:910	Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2.
34661088	4	26	theme	considerable	608:619	arg1	increase					621:628	the considerable increase	604:628	the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells	604:839	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	6	27	theme	recombinant	1182:1192	arg1	proteins					1197:1204	recombinant S1 proteins	1182:1204	recombinant S1 proteins	1182:1204	The results should contribute to future pathological studies of SARS-CoV-2 and therapeutic development of Covid-19, particularly using recombinant S1 proteins as models.
34661088	6	27	theme	recombinant	1182:1192	arg1	models					1209:1214	models	1209:1214	models	1209:1214	The results should contribute to future pathological studies of SARS-CoV-2 and therapeutic development of Covid-19, particularly using recombinant S1 proteins as models.
34661088	1	28	dep	spike	142:146	arg1	S					149:149	S	149:149	S	149:149	The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2).
34661088	3	29	theme	protein	482:488	arg1	N-glycosylation					460:474	different N-glycosylation	450:474	different N-glycosylation of S1 protein	450:488	In this study, we investigated the effect of different N-glycosylation of S1 protein on its binding to ACE2.
34661088	4	30	theme	binding	633:639	arg1	affinities					641:650	binding affinities	633:650	binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells	633:839	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	1	31	theme	host	256:259	arg1	entry					266:270	host cell entry	256:270	host cell entry	256:270	The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2).
34661088	3	32	from	effect	440:445	arg1	binding					497:503	its binding	493:503	its binding to ACE2	493:511	In this study, we investigated the effect of different N-glycosylation of S1 protein on its binding to ACE2.
34661088	4	33	theme	different	705:713	arg1	systems					726:732	three different expression systems	699:732	three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells	699:839	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	4	33	theme	different	705:713	arg1	ovarian					780:786	ovarian	780:786	ovarian	780:786	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	4	33	theme	different	705:713	arg1	variants					796:803	two variants	792:803	two variants of human embryonic kidney 293 cells	792:839	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	4	33	theme	different	705:713	arg1	baculovirus-insect					744:761	baculovirus-insect	744:761	baculovirus-insect	744:761	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	0	34	theme	N-glycosylation	14:28	arg1	effect					4:9	The effect	0:9	The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor.	0:115	The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor.
34661088	1	35	theme	cell	261:264	arg1	entry					266:270	host cell entry	256:270	host cell entry	256:270	The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2).
34661088	4	36	theme	embryonic	814:822	arg1	kidney					824:829	human embryonic kidney 293	808:833	human embryonic kidney 293 cells	808:839	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	5	37	theme	repulsion	956:964	arg1	effects					966:972	the steric hindrance and Coulombic repulsion effects	921:972	the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2	921:1044	Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2.
34661088	5	38	theme	protein	1016:1022	arg1	interaction					1024:1034	the S1 protein interaction	1009:1034	the S1 protein interaction with ACE2	1009:1044	Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2.
34661088	5	39	theme	Coulombic	946:954	arg1	repulsion					956:964	Coulombic repulsion	946:964	Coulombic repulsion	946:964	Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2.
34661088	6	40	theme	SARS-CoV-2	1111:1120	arg1	development					1138:1148	SARS-CoV-2 and therapeutic development	1111:1148	SARS-CoV-2 and therapeutic development of Covid-19, particularly using recombinant S1 proteins as models	1111:1214	The results should contribute to future pathological studies of SARS-CoV-2 and therapeutic development of Covid-19, particularly using recombinant S1 proteins as models.
34661088	0	41	theme	ACE2	102:105	arg1	receptor					107:114	the host cell ACE2 receptor	88:114	the host cell ACE2 receptor	88:114	The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor.
34661088	4	42	theme	de-N-glycosylated	655:671	arg1	proteins					676:683	de-N-glycosylated S1 proteins	655:683	de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells	655:839	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	0	43	theme	SARS-CoV-2	33:42	arg1	protein					50:56	SARS-CoV-2 spike protein	33:56	SARS-CoV-2 spike protein	33:56	The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor.
34661088	4	44	theme	human	808:812	arg1	kidney					824:829	human embryonic kidney 293	808:833	human embryonic kidney 293 cells	808:839	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	1	45	theme	severe	178:183	arg1	syndrome					203:210	severe acute respiratory syndrome	178:210	severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface	178:245	The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2).
34661088	6	46	theme	pathological	1087:1098	arg1	studies					1100:1106	future pathological studies	1080:1106	future pathological studies of SARS-CoV-2 and therapeutic development of Covid-19, particularly using recombinant S1 proteins as models	1080:1214	The results should contribute to future pathological studies of SARS-CoV-2 and therapeutic development of Covid-19, particularly using recombinant S1 proteins as models.
34661088	5	47	theme	Molecular	842:850	arg1	simulations					860:870	Molecular dynamic simulations	842:870	Molecular dynamic simulations of the S1 protein-ACE2 receptor complex	842:910	Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2.
34661088	1	48	theme	acute	185:189	arg1	syndrome					203:210	severe acute respiratory syndrome	178:210	severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface	178:245	The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2).
34661088	0	49	theme	protein	50:56	arg1	N-glycosylation					14:28	N-glycosylation	14:28	N-glycosylation of SARS-CoV-2 spike protein	14:56	The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor.
34661088	1	50	gly	glycosylated	129:140	arg1	protein					152:158	The densely glycosylated spike (S) protein	117:158	The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface	117:245	The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2).
34661088	4	51	theme	kidney	824:829	arg1	cells					835:839	human embryonic kidney 293 cells	808:839	human embryonic kidney 293 cells	808:839	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	6	52	theme	development	1138:1148	arg1	studies					1100:1106	future pathological studies	1080:1106	future pathological studies of SARS-CoV-2 and therapeutic development of Covid-19, particularly using recombinant S1 proteins as models	1080:1214	The results should contribute to future pathological studies of SARS-CoV-2 and therapeutic development of Covid-19, particularly using recombinant S1 proteins as models.
34661088	6	53	theme	Covid-19	1153:1160	arg1	development					1138:1148	SARS-CoV-2 and therapeutic development	1111:1148	SARS-CoV-2 and therapeutic development of Covid-19, particularly using recombinant S1 proteins as models	1111:1214	The results should contribute to future pathological studies of SARS-CoV-2 and therapeutic development of Covid-19, particularly using recombinant S1 proteins as models.
34661088	0	54	theme	spike	44:48	arg1	protein					50:56	SARS-CoV-2 spike protein	33:56	SARS-CoV-2 spike protein	33:56	The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor.
34661088	0	55	gly	N-glycosylation	14:28	arg1	virus					65:69	the virus interaction	61:81	the virus interaction with the host cell ACE2 receptor	61:114	The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor.
34661088	0	55	gly	N-glycosylation	14:28	arg1	protein					50:56	SARS-CoV-2 spike protein	33:56	SARS-CoV-2 spike protein	33:56	The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor.
34661088	4	56	theme	proteins	676:683	arg1	affinities					641:650	binding affinities	633:650	binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells	633:839	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	1	57	theme	receptor	290:297	arg1	enzyme					322:327	the receptor angiotensin-converting enzyme 2	286:329	the receptor angiotensin-converting enzyme 2 (ACE2)	286:336	The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2).
34661088	1	57	theme	receptor	290:297	arg1	ACE2					332:335	ACE2	332:335	ACE2	332:335	The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2).
34661088	4	58	gly	de-N-glycosylated	655:671	arg1	proteins					676:683	de-N-glycosylated S1 proteins	655:683	de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells	655:839	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	5	59	theme	N-glycans	996:1004	arg1	types					987:991	different types	977:991	different types of N-glycans	977:1004	Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2.
34661088	4	60	theme	affinities	641:650	arg1	increase					621:628	the considerable increase	604:628	the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells	604:839	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
34661088	5	61	theme	steric	925:930	arg1	hindrance					932:940	steric hindrance	925:940	steric hindrance	925:940	Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2.
34661088	5	62	from	effects	966:972	arg1	interaction					1024:1034	the S1 protein interaction	1009:1034	the S1 protein interaction with ACE2	1009:1044	Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2.
34661088	1	63	theme	respiratory	191:201	arg1	syndrome					203:210	severe acute respiratory syndrome	178:210	severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface	178:245	The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2).
34661088	6	64	theme	S1	1194:1195	arg1	proteins					1197:1204	recombinant S1 proteins	1182:1204	recombinant S1 proteins	1182:1204	The results should contribute to future pathological studies of SARS-CoV-2 and therapeutic development of Covid-19, particularly using recombinant S1 proteins as models.
34661088	6	64	theme	S1	1194:1195	arg1	models					1209:1214	models	1209:1214	models	1209:1214	The results should contribute to future pathological studies of SARS-CoV-2 and therapeutic development of Covid-19, particularly using recombinant S1 proteins as models.
34661088	5	65	theme	types	987:991	arg1	effects					966:972	the steric hindrance and Coulombic repulsion effects	921:972	the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2	921:1044	Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2.
34661088	3	66	theme	S1	479:480	arg1	protein					482:488	S1 protein	479:488	S1 protein	479:488	In this study, we investigated the effect of different N-glycosylation of S1 protein on its binding to ACE2.
34661088	1	67	theme	syndrome	203:210	arg1	surface					239:245	severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface	178:245	severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface	178:245	The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2).
34661088	2	68	theme	glycosylation	360:372	arg1	role					352:355	the role	348:355	the role of glycosylation	348:372	However, the role of glycosylation has not been fully understood.
34661088	5	69	theme	S1	879:880	arg1	complex					904:910	the S1 protein-ACE2 receptor complex	875:910	the S1 protein-ACE2 receptor complex	875:910	Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2.
34661088	6	70	theme	future	1080:1085	arg1	studies					1100:1106	future pathological studies	1080:1106	future pathological studies of SARS-CoV-2 and therapeutic development of Covid-19, particularly using recombinant S1 proteins as models	1080:1214	The results should contribute to future pathological studies of SARS-CoV-2 and therapeutic development of Covid-19, particularly using recombinant S1 proteins as models.
34661088	1	71	theme	coronavirus-2	212:224	arg1	surface					239:245	severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface	178:245	severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface	178:245	The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2).
34661088	1	72	theme	angiotensin-converting	299:320	arg1	enzyme					322:327	the receptor angiotensin-converting enzyme 2	286:329	the receptor angiotensin-converting enzyme 2 (ACE2)	286:336	The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2).
34661088	1	72	theme	angiotensin-converting	299:320	arg1	ACE2					332:335	ACE2	332:335	ACE2	332:335	The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2).
34661088	5	73	theme	S1	1013:1014	arg1	interaction					1024:1034	the S1 protein interaction	1009:1034	the S1 protein interaction with ACE2	1009:1044	Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2.
34661088	1	74	theme	glycosylated	129:140	arg1	protein					152:158	The densely glycosylated spike (S) protein	117:158	The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface	117:245	The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2).
34661088	4	75	theme	negative	566:573	arg1	effects					575:581	the negative effects	562:581	the negative effects	562:581	Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells.
32773734	4	0	theme	SF/Cs/Alg	759:767	arg1	scaffolds					746:754	triple composite scaffolds	729:754	triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2	729:812	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	4	1	theme	cells	835:839	arg1	culture					821:827	3D culture	818:827	3D culture of CC cells	818:839	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	3	2	theme	silk	623:626	arg1	SF					637:638	SF	637:638	SF	637:638	To improve the performance of the scaffolds for 3D cell culture of colonic carcinoma (CC) cells in vitro, we attempted to construct triple composite scaffolds using silk fibroin (SF), chitosan (Cs), and alginate (Alg).
32773734	3	2	theme	silk	623:626	arg1	fibroin					628:634	silk fibroin	623:634	silk fibroin (SF)	623:639	To improve the performance of the scaffolds for 3D cell culture of colonic carcinoma (CC) cells in vitro, we attempted to construct triple composite scaffolds using silk fibroin (SF), chitosan (Cs), and alginate (Alg).
32773734	3	3	theme	3D	506:507	arg1	culture					514:520	3D cell culture	506:520	3D cell culture of colonic carcinoma (CC) cells	506:552	To improve the performance of the scaffolds for 3D cell culture of colonic carcinoma (CC) cells in vitro, we attempted to construct triple composite scaffolds using silk fibroin (SF), chitosan (Cs), and alginate (Alg).
32773734	5	4	theme	scaffolds	972:980	arg1	characteristics					947:961	the physicochemical characteristics	927:961	the physicochemical characteristics of these scaffolds	927:980	We analyzed the physicochemical characteristics of these scaffolds and studied cell adhesion, cell proliferation, migration, colony-forming ability, microstructure and ultrastructure, and spheroid-forming capacity of the commercially available CC cell line HCT-116 on the prepared scaffolds.
32773734	8	5	theme	in	2068:2069	arg1	culture					2077:2083	the 3D in vitro culture	2061:2083	the 3D in vitro culture of CC cells	2061:2095	CONCLUSIONS This study successfully demonstrated the potential of SF/Cs/Alg (1: 1: 1) scaffold as an alternative for the 3D in vitro culture of CC cells.
32773734	4	6	theme	triple	729:734	arg1	scaffolds					746:754	triple composite scaffolds	729:754	triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2	729:812	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	7	7	theme	cell	1575:1578	arg1	imaging					1580:1586	green fluorescent protein-labeled HCT116 cell imaging	1534:1586	green fluorescent protein-labeled HCT116 cell imaging	1534:1586	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	7	theme	cell	1575:1578	arg1	assays					1526:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	8	8	dep	CONCLUSIONS	1944:1954	arg1	demonstrated					1980:1991	demonstrated	1980:1991	demonstrated the potential of SF/Cs/Alg (1: 1: 1) scaffold as an alternative for the 3D in vitro culture of CC cells	1980:2095	CONCLUSIONS This study successfully demonstrated the potential of SF/Cs/Alg (1: 1: 1) scaffold as an alternative for the 3D in vitro culture of CC cells.
32773734	7	9	theme	SF/CS	1930:1934	arg1	scaffold					1918:1925	the dual composite scaffold	1899:1925	the dual composite scaffold of SF/CS (1: 1)	1899:1941	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	3	10	theme	scaffolds	492:500	arg1	performance					473:483	the performance	469:483	the performance of the scaffolds for 3D cell culture of colonic carcinoma (CC) cells	469:552	To improve the performance of the scaffolds for 3D cell culture of colonic carcinoma (CC) cells in vitro, we attempted to construct triple composite scaffolds using silk fibroin (SF), chitosan (Cs), and alginate (Alg).
32773734	6	11	theme	best	1284:1287	arg1	profile					1289:1295	profile	1289:1295	profile	1289:1295	RESULTS Our results show that SF/Cs/Alg (1: 1: 1) scaffolds demonstrated the best profile, the highest uniform porosity and connectivity, and excellent hydroscopicity, and also exhibited appropriate and controlled swelling and degradation characteristics.
32773734	4	12	theme	scaffolds	746:754	arg1	suitability					714:724	the suitability	710:724	the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells	710:839	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	7	13	theme	protein-labeled	1552:1566	arg1	imaging					1580:1586	green fluorescent protein-labeled HCT116 cell imaging	1534:1586	green fluorescent protein-labeled HCT116 cell imaging	1534:1586	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	13	theme	protein-labeled	1552:1566	arg1	assays					1526:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	5	14	theme	prepared	1187:1194	arg1	scaffolds					1196:1204	the prepared scaffolds	1183:1204	the prepared scaffolds	1183:1204	We analyzed the physicochemical characteristics of these scaffolds and studied cell adhesion, cell proliferation, migration, colony-forming ability, microstructure and ultrastructure, and spheroid-forming capacity of the commercially available CC cell line HCT-116 on the prepared scaffolds.
32773734	3	15	theme	colonic	525:531	arg1	cells					548:552	colonic carcinoma (CC) cells	525:552	colonic carcinoma (CC) cells	525:552	To improve the performance of the scaffolds for 3D cell culture of colonic carcinoma (CC) cells in vitro, we attempted to construct triple composite scaffolds using silk fibroin (SF), chitosan (Cs), and alginate (Alg).
32773734	8	16	theme	CC	2088:2089	arg1	cells					2091:2095	CC cells	2088:2095	CC cells	2088:2095	CONCLUSIONS This study successfully demonstrated the potential of SF/Cs/Alg (1: 1: 1) scaffold as an alternative for the 3D in vitro culture of CC cells.
32773734	6	17	dep	profile	1289:1295	arg1	the					1280:1282	the	1280:1282	the	1280:1282	RESULTS Our results show that SF/Cs/Alg (1: 1: 1) scaffolds demonstrated the best profile, the highest uniform porosity and connectivity, and excellent hydroscopicity, and also exhibited appropriate and controlled swelling and degradation characteristics.
32773734	7	18	theme	dual	1903:1906	arg1	scaffold					1918:1925	the dual composite scaffold	1899:1925	the dual composite scaffold of SF/CS (1: 1)	1899:1941	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	4	19	theme	1	793:793	arg1	ratios					772:777	ratios	772:777	ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2	772:812	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	0	20	theme	3D	122:123	arg1	Culture					125:131	3D Culture	122:131	3D Culture of Colonic Carcinoma Cells	122:158	Preparation and Characterization of a Novel Triple Composite Scaffold Containing Silk Fiborin, Chitosan, and Alginate for 3D Culture of Colonic Carcinoma Cells In Vitro.
32773734	7	21	theme	SF/CS/Alg	1765:1773	arg1	scaffolds					1752:1760	the triple composite scaffolds	1731:1760	the triple composite scaffolds of SF/CS/Alg (1: 1: 1)	1731:1783	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	22	theme	wound-healing	1512:1524	arg1	assays					1526:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	22	theme	wound-healing	1512:1524	arg1	microscopy					1669:1678	scanning electron microscopy	1651:1678	scanning electron microscopy	1651:1678	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	22	theme	wound-healing	1512:1524	arg1	imaging					1580:1586	green fluorescent protein-labeled HCT116 cell imaging	1534:1586	green fluorescent protein-labeled HCT116 cell imaging	1534:1586	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	22	theme	wound-healing	1512:1524	arg1	staining					1641:1648	6-diamidino-2-phenylindole and DY-554-phalloidin staining	1592:1648	staining	1641:1648	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	22	theme	wound-healing	1512:1524	arg1	haematoxylin					1685:1696	haematoxylin	1685:1696	haematoxylin	1685:1696	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	22	theme	wound-healing	1512:1524	arg1	4					1589:1589	4	1589:1589	4	1589:1589	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	8	23	theme	SF/Cs/Alg	2010:2018	arg1	scaffold					2030:2037	SF/Cs/Alg (1: 1: 1) scaffold	2010:2037	SF/Cs/Alg (1: 1: 1) scaffold	2010:2037	CONCLUSIONS This study successfully demonstrated the potential of SF/Cs/Alg (1: 1: 1) scaffold as an alternative for the 3D in vitro culture of CC cells.
32773734	7	24	theme	spheroid	1864:1871	arg1	formation					1873:1881	spheroid formation	1864:1881	spheroid formation	1864:1881	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	0	25	theme	Carcinoma	144:152	arg1	Cells					154:158	Colonic Carcinoma Cells	136:158	Colonic Carcinoma Cells	136:158	Preparation and Characterization of a Novel Triple Composite Scaffold Containing Silk Fiborin, Chitosan, and Alginate for 3D Culture of Colonic Carcinoma Cells In Vitro.
32773734	6	26	theme	controlled	1410:1419	arg1	characteristics					1446:1460	appropriate and controlled swelling and degradation characteristics	1394:1460	appropriate and controlled swelling and degradation characteristics	1394:1460	RESULTS Our results show that SF/Cs/Alg (1: 1: 1) scaffolds demonstrated the best profile, the highest uniform porosity and connectivity, and excellent hydroscopicity, and also exhibited appropriate and controlled swelling and degradation characteristics.
32773734	0	27	theme	Scaffold	61:68	arg1	Characterization					16:31	Characterization	16:31	Characterization	16:31	Preparation and Characterization of a Novel Triple Composite Scaffold Containing Silk Fiborin, Chitosan, and Alginate for 3D Culture of Colonic Carcinoma Cells In Vitro.
32773734	0	27	theme	Scaffold	61:68	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and Characterization of a Novel Triple Composite Scaffold Containing Silk Fiborin, Chitosan, and Alginate for 3D Culture of Colonic Carcinoma Cells In Vitro.
32773734	4	28	from	ratios	772:777	arg1	SF/Cs/Alg					759:767	SF/Cs/Alg	759:767	SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2	759:812	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	4	28	from	ratios	772:777	arg1	scaffolds					746:754	triple composite scaffolds	729:754	triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2	729:812	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	6	29	theme	highest	1302:1308	arg1	porosity					1318:1325	the highest uniform porosity	1298:1325	the highest uniform porosity	1298:1325	RESULTS Our results show that SF/Cs/Alg (1: 1: 1) scaffolds demonstrated the best profile, the highest uniform porosity and connectivity, and excellent hydroscopicity, and also exhibited appropriate and controlled swelling and degradation characteristics.
32773734	7	30	theme	colony-forming	1836:1849	arg1	ability					1851:1857	colony-forming ability	1836:1857	colony-forming ability	1836:1857	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	5	31	theme	cell	1009:1012	arg1	proliferation					1014:1026	cell proliferation	1009:1026	cell proliferation	1009:1026	We analyzed the physicochemical characteristics of these scaffolds and studied cell adhesion, cell proliferation, migration, colony-forming ability, microstructure and ultrastructure, and spheroid-forming capacity of the commercially available CC cell line HCT-116 on the prepared scaffolds.
32773734	0	32	theme	Silk	81:84	arg1	Fiborin					86:92	Silk Fiborin	81:92	Silk Fiborin	81:92	Preparation and Characterization of a Novel Triple Composite Scaffold Containing Silk Fiborin, Chitosan, and Alginate for 3D Culture of Colonic Carcinoma Cells In Vitro.
32773734	1	33	theme	in	321:322	arg1	models					336:341	two-dimensional cell culture in vitro and in vivo cancer models	279:341	two-dimensional cell culture in vitro and in vivo cancer models	279:341	BACKGROUND Three-dimensional (3D) cell-culture scaffolds are ideal in vitro models to bridge the gap between two-dimensional cell culture in vitro and in vivo cancer models.
32773734	1	34	dep	in	237:238	arg1	vitro					240:244	vitro	240:244	vitro	240:244	BACKGROUND Three-dimensional (3D) cell-culture scaffolds are ideal in vitro models to bridge the gap between two-dimensional cell culture in vitro and in vivo cancer models.
32773734	4	35	theme	1	796:796	arg1	ratios					772:777	ratios	772:777	ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2	772:812	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	4	36	from	scaffolds	746:754	arg1	ratios					772:777	ratios	772:777	ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2	772:812	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	7	37	theme	composite	1742:1750	arg1	scaffolds					1752:1760	the triple composite scaffolds	1731:1760	the triple composite scaffolds of SF/CS/Alg (1: 1: 1)	1731:1783	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	4	38	theme	1	799:799	arg1	ratios					772:777	ratios	772:777	ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2	772:812	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	1	39	dep	in	308:309	arg1	vitro					311:315	vitro	311:315	vitro	311:315	BACKGROUND Three-dimensional (3D) cell-culture scaffolds are ideal in vitro models to bridge the gap between two-dimensional cell culture in vitro and in vivo cancer models.
32773734	2	40	theme	3D	360:361	arg1	scaffolds					363:371	3D scaffolds	360:371	3D scaffolds using two kinds of biomaterials	360:403	Construction of 3D scaffolds using two kinds of biomaterials has been reported, but there are still many defects.
32773734	4	41	used	used	846:849	arg2	MATERIAL					677:684	MATERIAL	677:684	MATERIAL	677:684	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	4	42	theme	CC	832:833	arg1	cells					835:839	CC cells	832:839	CC cells	832:839	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	2	43	theme	biomaterials	392:403	arg1	kinds					383:387	two kinds	379:387	two kinds of biomaterials	379:403	Construction of 3D scaffolds using two kinds of biomaterials has been reported, but there are still many defects.
32773734	7	44	theme	eosin	1702:1706	arg1	staining					1708:1715	eosin staining	1702:1715	eosin staining	1702:1715	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	1	45	theme	two-dimensional	279:293	arg1	models					336:341	two-dimensional cell culture in vitro and in vivo cancer models	279:341	two-dimensional cell culture in vitro and in vivo cancer models	279:341	BACKGROUND Three-dimensional (3D) cell-culture scaffolds are ideal in vitro models to bridge the gap between two-dimensional cell culture in vitro and in vivo cancer models.
32773734	5	46	theme	cell	1162:1165	arg1	line					1167:1170	the commercially available CC cell line HCT-116	1132:1178	the commercially available CC cell line HCT-116	1132:1178	We analyzed the physicochemical characteristics of these scaffolds and studied cell adhesion, cell proliferation, migration, colony-forming ability, microstructure and ultrastructure, and spheroid-forming capacity of the commercially available CC cell line HCT-116 on the prepared scaffolds.
32773734	0	47	theme	Triple	44:49	arg1	Scaffold					61:68	a Novel Triple Composite Scaffold	36:68	a Novel Triple Composite Scaffold	36:68	Preparation and Characterization of a Novel Triple Composite Scaffold Containing Silk Fiborin, Chitosan, and Alginate for 3D Culture of Colonic Carcinoma Cells In Vitro.
32773734	1	48	theme	culture	300:306	arg1	models					336:341	two-dimensional cell culture in vitro and in vivo cancer models	279:341	two-dimensional cell culture in vitro and in vivo cancer models	279:341	BACKGROUND Three-dimensional (3D) cell-culture scaffolds are ideal in vitro models to bridge the gap between two-dimensional cell culture in vitro and in vivo cancer models.
32773734	8	49	theme	scaffold	2030:2037	arg1	potential					1997:2005	the potential	1993:2005	the potential of SF/Cs/Alg (1: 1: 1) scaffold as an alternative for the 3D in vitro culture of CC cells	1993:2095	CONCLUSIONS This study successfully demonstrated the potential of SF/Cs/Alg (1: 1: 1) scaffold as an alternative for the 3D in vitro culture of CC cells.
32773734	4	50	theme	dual	855:858	arg1	group					908:912	a control group	898:912	a control group	898:912	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	4	50	theme	dual	855:858	arg1	scaffold					870:877	the dual composite scaffold	851:877	the dual composite scaffold of SF/Cs (1: 1)	851:893	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	1	51	theme	cell-culture	204:215	arg1	models					246:251	ideal in vitro models	231:251	ideal in vitro models	231:251	BACKGROUND Three-dimensional (3D) cell-culture scaffolds are ideal in vitro models to bridge the gap between two-dimensional cell culture in vitro and in vivo cancer models.
32773734	1	51	theme	cell-culture	204:215	arg1	scaffolds					217:225	BACKGROUND Three-dimensional (3D) cell-culture scaffolds	170:225	BACKGROUND Three-dimensional (3D) cell-culture scaffolds	170:225	BACKGROUND Three-dimensional (3D) cell-culture scaffolds are ideal in vitro models to bridge the gap between two-dimensional cell culture in vitro and in vivo cancer models.
32773734	7	52	theme	electron	1660:1667	arg1	microscopy					1669:1678	scanning electron microscopy	1651:1678	scanning electron microscopy	1651:1678	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	52	theme	electron	1660:1667	arg1	assays					1526:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	3	53	theme	composite	597:605	arg1	scaffolds					607:615	triple composite scaffolds	590:615	triple composite scaffolds using silk fibroin (SF), chitosan (Cs), and alginate (Alg)	590:674	To improve the performance of the scaffolds for 3D cell culture of colonic carcinoma (CC) cells in vitro, we attempted to construct triple composite scaffolds using silk fibroin (SF), chitosan (Cs), and alginate (Alg).
32773734	1	54	dep	Three-dimensional	181:197	arg1	3D					200:201	3D	200:201	3D	200:201	BACKGROUND Three-dimensional (3D) cell-culture scaffolds are ideal in vitro models to bridge the gap between two-dimensional cell culture in vitro and in vivo cancer models.
32773734	5	55	theme	cell	994:997	arg1	adhesion					999:1006	cell adhesion	994:1006	cell adhesion	994:1006	We analyzed the physicochemical characteristics of these scaffolds and studied cell adhesion, cell proliferation, migration, colony-forming ability, microstructure and ultrastructure, and spheroid-forming capacity of the commercially available CC cell line HCT-116 on the prepared scaffolds.
32773734	5	56	theme	available	1149:1157	arg1	line					1167:1170	the commercially available CC cell line HCT-116	1132:1178	the commercially available CC cell line HCT-116	1132:1178	We analyzed the physicochemical characteristics of these scaffolds and studied cell adhesion, cell proliferation, migration, colony-forming ability, microstructure and ultrastructure, and spheroid-forming capacity of the commercially available CC cell line HCT-116 on the prepared scaffolds.
32773734	1	57	theme	in	237:238	arg1	models					246:251	ideal in vitro models	231:251	ideal in vitro models	231:251	BACKGROUND Three-dimensional (3D) cell-culture scaffolds are ideal in vitro models to bridge the gap between two-dimensional cell culture in vitro and in vivo cancer models.
32773734	1	57	theme	in	237:238	arg1	scaffolds					217:225	BACKGROUND Three-dimensional (3D) cell-culture scaffolds	170:225	BACKGROUND Three-dimensional (3D) cell-culture scaffolds	170:225	BACKGROUND Three-dimensional (3D) cell-culture scaffolds are ideal in vitro models to bridge the gap between two-dimensional cell culture in vitro and in vivo cancer models.
32773734	8	58	theme	3D	2065:2066	arg1	culture					2077:2083	the 3D in vitro culture	2061:2083	the 3D in vitro culture of CC cells	2061:2095	CONCLUSIONS This study successfully demonstrated the potential of SF/Cs/Alg (1: 1: 1) scaffold as an alternative for the 3D in vitro culture of CC cells.
32773734	7	59	theme	HCT116	1568:1573	arg1	imaging					1580:1586	green fluorescent protein-labeled HCT116 cell imaging	1534:1586	green fluorescent protein-labeled HCT116 cell imaging	1534:1586	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	59	theme	HCT116	1568:1573	arg1	assays					1526:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	60	theme	colony-forming	1492:1505	arg1	adhesion					1467:1474	The adhesion	1463:1474	The adhesion	1463:1474	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	61	theme	cell	1795:1798	arg1	adhesion					1800:1807	cell adhesion	1795:1807	cell adhesion	1795:1807	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	62	theme	fluorescent	1540:1550	arg1	imaging					1580:1586	green fluorescent protein-labeled HCT116 cell imaging	1534:1586	green fluorescent protein-labeled HCT116 cell imaging	1534:1586	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	62	theme	fluorescent	1540:1550	arg1	assays					1526:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	2	63	theme	many	444:447	arg1	defects					449:455	many defects	444:455	many defects	444:455	Construction of 3D scaffolds using two kinds of biomaterials has been reported, but there are still many defects.
32773734	4	64	dep	MATERIAL	677:684	arg1	We					698:699	We	698:699	We	698:699	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	8	65	theme	cells	2091:2095	arg1	culture					2077:2083	the 3D in vitro culture	2061:2083	the 3D in vitro culture of CC cells	2061:2095	CONCLUSIONS This study successfully demonstrated the potential of SF/Cs/Alg (1: 1: 1) scaffold as an alternative for the 3D in vitro culture of CC cells.
32773734	4	66	theme	composite	736:744	arg1	scaffolds					746:754	triple composite scaffolds	729:754	triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2	729:812	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	4	67	theme	SF/Cs	882:886	arg1	group					908:912	a control group	898:912	a control group	898:912	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	4	67	theme	SF/Cs	882:886	arg1	scaffold					870:877	the dual composite scaffold	851:877	the dual composite scaffold of SF/Cs (1: 1)	851:893	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	3	68	theme	cell	509:512	arg1	culture					514:520	3D cell culture	506:520	3D cell culture of colonic carcinoma (CC) cells	506:552	To improve the performance of the scaffolds for 3D cell culture of colonic carcinoma (CC) cells in vitro, we attempted to construct triple composite scaffolds using silk fibroin (SF), chitosan (Cs), and alginate (Alg).
32773734	1	69	theme	Three-dimensional	181:197	arg1	models					246:251	ideal in vitro models	231:251	ideal in vitro models	231:251	BACKGROUND Three-dimensional (3D) cell-culture scaffolds are ideal in vitro models to bridge the gap between two-dimensional cell culture in vitro and in vivo cancer models.
32773734	1	69	theme	Three-dimensional	181:197	arg1	scaffolds					217:225	BACKGROUND Three-dimensional (3D) cell-culture scaffolds	170:225	BACKGROUND Three-dimensional (3D) cell-culture scaffolds	170:225	BACKGROUND Three-dimensional (3D) cell-culture scaffolds are ideal in vitro models to bridge the gap between two-dimensional cell culture in vitro and in vivo cancer models.
32773734	7	70	theme	composite	1908:1916	arg1	scaffold					1918:1925	the dual composite scaffold	1899:1925	the dual composite scaffold of SF/CS (1: 1)	1899:1941	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	3	71	theme	CC	544:545	arg1	cells					548:552	colonic carcinoma (CC) cells	525:552	colonic carcinoma (CC) cells	525:552	To improve the performance of the scaffolds for 3D cell culture of colonic carcinoma (CC) cells in vitro, we attempted to construct triple composite scaffolds using silk fibroin (SF), chitosan (Cs), and alginate (Alg).
32773734	0	72	theme	Colonic	136:142	arg1	Cells					154:158	Colonic Carcinoma Cells	136:158	Colonic Carcinoma Cells	136:158	Preparation and Characterization of a Novel Triple Composite Scaffold Containing Silk Fiborin, Chitosan, and Alginate for 3D Culture of Colonic Carcinoma Cells In Vitro.
32773734	3	73	theme	carcinoma	533:541	arg1	cells					548:552	colonic carcinoma (CC) cells	525:552	colonic carcinoma (CC) cells	525:552	To improve the performance of the scaffolds for 3D cell culture of colonic carcinoma (CC) cells in vitro, we attempted to construct triple composite scaffolds using silk fibroin (SF), chitosan (Cs), and alginate (Alg).
32773734	6	74	theme	degradation	1434:1444	arg1	characteristics					1446:1460	appropriate and controlled swelling and degradation characteristics	1394:1460	appropriate and controlled swelling and degradation characteristics	1394:1460	RESULTS Our results show that SF/Cs/Alg (1: 1: 1) scaffolds demonstrated the best profile, the highest uniform porosity and connectivity, and excellent hydroscopicity, and also exhibited appropriate and controlled swelling and degradation characteristics.
32773734	7	75	theme	better	1887:1892	arg1	adhesion					1800:1807	cell adhesion	1795:1807	cell adhesion	1795:1807	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	76	theme	green	1534:1538	arg1	imaging					1580:1586	green fluorescent protein-labeled HCT116 cell imaging	1534:1586	green fluorescent protein-labeled HCT116 cell imaging	1534:1586	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	76	theme	green	1534:1538	arg1	assays					1526:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	0	77	theme	Cells	154:158	arg1	Culture					125:131	3D Culture	122:131	3D Culture of Colonic Carcinoma Cells	122:158	Preparation and Characterization of a Novel Triple Composite Scaffold Containing Silk Fiborin, Chitosan, and Alginate for 3D Culture of Colonic Carcinoma Cells In Vitro.
32773734	6	78	dep	SF/Cs/Alg	1237:1245	arg1	scaffolds					1257:1265	scaffolds	1257:1265	scaffolds	1257:1265	RESULTS Our results show that SF/Cs/Alg (1: 1: 1) scaffolds demonstrated the best profile, the highest uniform porosity and connectivity, and excellent hydroscopicity, and also exhibited appropriate and controlled swelling and degradation characteristics.
32773734	6	79	theme	swelling	1421:1428	arg1	characteristics					1446:1460	appropriate and controlled swelling and degradation characteristics	1394:1460	appropriate and controlled swelling and degradation characteristics	1394:1460	RESULTS Our results show that SF/Cs/Alg (1: 1: 1) scaffolds demonstrated the best profile, the highest uniform porosity and connectivity, and excellent hydroscopicity, and also exhibited appropriate and controlled swelling and degradation characteristics.
32773734	4	80	theme	control	900:906	arg1	group					908:912	a control group	898:912	a control group	898:912	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	4	80	theme	control	900:906	arg1	scaffold					870:877	the dual composite scaffold	851:877	the dual composite scaffold of SF/Cs (1: 1)	851:893	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	5	81	theme	colony-forming	1040:1053	arg1	ability					1055:1061	colony-forming ability	1040:1061	colony-forming ability	1040:1061	We analyzed the physicochemical characteristics of these scaffolds and studied cell adhesion, cell proliferation, migration, colony-forming ability, microstructure and ultrastructure, and spheroid-forming capacity of the commercially available CC cell line HCT-116 on the prepared scaffolds.
32773734	3	82	theme	cells	548:552	arg1	culture					514:520	3D cell culture	506:520	3D cell culture of colonic carcinoma (CC) cells	506:552	To improve the performance of the scaffolds for 3D cell culture of colonic carcinoma (CC) cells in vitro, we attempted to construct triple composite scaffolds using silk fibroin (SF), chitosan (Cs), and alginate (Alg).
32773734	6	83	theme	uniform	1310:1316	arg1	porosity					1318:1325	the highest uniform porosity	1298:1325	the highest uniform porosity	1298:1325	RESULTS Our results show that SF/Cs/Alg (1: 1: 1) scaffolds demonstrated the best profile, the highest uniform porosity and connectivity, and excellent hydroscopicity, and also exhibited appropriate and controlled swelling and degradation characteristics.
32773734	8	84	dep	in	2068:2069	arg1	vitro					2071:2075	vitro	2071:2075	vitro	2071:2075	CONCLUSIONS This study successfully demonstrated the potential of SF/Cs/Alg (1: 1: 1) scaffold as an alternative for the 3D in vitro culture of CC cells.
32773734	1	85	theme	cancer	329:334	arg1	models					336:341	two-dimensional cell culture in vitro and in vivo cancer models	279:341	two-dimensional cell culture in vitro and in vivo cancer models	279:341	BACKGROUND Three-dimensional (3D) cell-culture scaffolds are ideal in vitro models to bridge the gap between two-dimensional cell culture in vitro and in vivo cancer models.
32773734	4	86	theme	1	806:806	arg1	ratios					772:777	ratios	772:777	ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2	772:812	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	4	87	dep	1	793:793	arg1	2					812:812	2	812:812	2	812:812	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	4	87	dep	1	793:793	arg1	1					809:809	1	809:809	1	809:809	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	7	88	theme	triple	1735:1740	arg1	scaffolds					1752:1760	the triple composite scaffolds	1731:1760	the triple composite scaffolds of SF/CS/Alg (1: 1: 1)	1731:1783	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	6	89	theme	appropriate	1394:1404	arg1	characteristics					1446:1460	appropriate and controlled swelling and degradation characteristics	1394:1460	appropriate and controlled swelling and degradation characteristics	1394:1460	RESULTS Our results show that SF/Cs/Alg (1: 1: 1) scaffolds demonstrated the best profile, the highest uniform porosity and connectivity, and excellent hydroscopicity, and also exhibited appropriate and controlled swelling and degradation characteristics.
32773734	7	90	theme	proliferation	1477:1489	arg1	assays					1526:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	90	theme	proliferation	1477:1489	arg1	microscopy					1669:1678	scanning electron microscopy	1651:1678	scanning electron microscopy	1651:1678	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	90	theme	proliferation	1477:1489	arg1	imaging					1580:1586	green fluorescent protein-labeled HCT116 cell imaging	1534:1586	green fluorescent protein-labeled HCT116 cell imaging	1534:1586	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	90	theme	proliferation	1477:1489	arg1	staining					1641:1648	6-diamidino-2-phenylindole and DY-554-phalloidin staining	1592:1648	staining	1641:1648	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	90	theme	proliferation	1477:1489	arg1	haematoxylin					1685:1696	haematoxylin	1685:1696	haematoxylin	1685:1696	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	90	theme	proliferation	1477:1489	arg1	4					1589:1589	4	1589:1589	4	1589:1589	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	91	theme	adhesion	1467:1474	arg1	assays					1526:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	91	theme	adhesion	1467:1474	arg1	microscopy					1669:1678	scanning electron microscopy	1651:1678	scanning electron microscopy	1651:1678	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	91	theme	adhesion	1467:1474	arg1	imaging					1580:1586	green fluorescent protein-labeled HCT116 cell imaging	1534:1586	green fluorescent protein-labeled HCT116 cell imaging	1534:1586	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	91	theme	adhesion	1467:1474	arg1	staining					1641:1648	6-diamidino-2-phenylindole and DY-554-phalloidin staining	1592:1648	staining	1641:1648	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	91	theme	adhesion	1467:1474	arg1	haematoxylin					1685:1696	haematoxylin	1685:1696	haematoxylin	1685:1696	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	91	theme	adhesion	1467:1474	arg1	4					1589:1589	4	1589:1589	4	1589:1589	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	5	92	theme	spheroid-forming	1103:1118	arg1	capacity					1120:1127	spheroid-forming capacity	1103:1127	spheroid-forming capacity of the commercially available CC cell line HCT-116	1103:1178	We analyzed the physicochemical characteristics of these scaffolds and studied cell adhesion, cell proliferation, migration, colony-forming ability, microstructure and ultrastructure, and spheroid-forming capacity of the commercially available CC cell line HCT-116 on the prepared scaffolds.
32773734	2	93	theme	scaffolds	363:371	arg1	Construction					344:355	Construction	344:355	Construction of 3D scaffolds using two kinds of biomaterials	344:403	Construction of 3D scaffolds using two kinds of biomaterials has been reported, but there are still many defects.
32773734	4	94	theme	3D	818:819	arg1	culture					821:827	3D culture	818:827	3D culture of CC cells	818:839	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	1	95	dep	in	321:322	arg1	vivo					324:327	vivo	324:327	vivo	324:327	BACKGROUND Three-dimensional (3D) cell-culture scaffolds are ideal in vitro models to bridge the gap between two-dimensional cell culture in vitro and in vivo cancer models.
32773734	6	96	theme	excellent	1349:1357	arg1	hydroscopicity					1359:1372	excellent hydroscopicity	1349:1372	excellent hydroscopicity	1349:1372	RESULTS Our results show that SF/Cs/Alg (1: 1: 1) scaffolds demonstrated the best profile, the highest uniform porosity and connectivity, and excellent hydroscopicity, and also exhibited appropriate and controlled swelling and degradation characteristics.
32773734	0	97	theme	Novel	38:42	arg1	Scaffold					61:68	a Novel Triple Composite Scaffold	36:68	a Novel Triple Composite Scaffold	36:68	Preparation and Characterization of a Novel Triple Composite Scaffold Containing Silk Fiborin, Chitosan, and Alginate for 3D Culture of Colonic Carcinoma Cells In Vitro.
32773734	5	98	theme	physicochemical	931:945	arg1	characteristics					947:961	the physicochemical characteristics	927:961	the physicochemical characteristics of these scaffolds	927:980	We analyzed the physicochemical characteristics of these scaffolds and studied cell adhesion, cell proliferation, migration, colony-forming ability, microstructure and ultrastructure, and spheroid-forming capacity of the commercially available CC cell line HCT-116 on the prepared scaffolds.
32773734	5	99	theme	CC	1159:1160	arg1	line					1167:1170	the commercially available CC cell line HCT-116	1132:1178	the commercially available CC cell line HCT-116	1132:1178	We analyzed the physicochemical characteristics of these scaffolds and studied cell adhesion, cell proliferation, migration, colony-forming ability, microstructure and ultrastructure, and spheroid-forming capacity of the commercially available CC cell line HCT-116 on the prepared scaffolds.
32773734	0	100	theme	Composite	51:59	arg1	Scaffold					61:68	a Novel Triple Composite Scaffold	36:68	a Novel Triple Composite Scaffold	36:68	Preparation and Characterization of a Novel Triple Composite Scaffold Containing Silk Fiborin, Chitosan, and Alginate for 3D Culture of Colonic Carcinoma Cells In Vitro.
32773734	1	101	theme	cell	295:298	arg1	models					336:341	two-dimensional cell culture in vitro and in vivo cancer models	279:341	two-dimensional cell culture in vitro and in vivo cancer models	279:341	BACKGROUND Three-dimensional (3D) cell-culture scaffolds are ideal in vitro models to bridge the gap between two-dimensional cell culture in vitro and in vivo cancer models.
32773734	6	102	dep	RESULTS	1207:1213	arg1	show					1227:1230	show	1227:1230	show that SF/Cs/Alg (1: 1: 1) scaffolds demonstrated the best profile, the highest uniform porosity and connectivity, and excellent hydroscopicity, and also exhibited appropriate and controlled swelling and degradation characteristics	1227:1460	RESULTS Our results show that SF/Cs/Alg (1: 1: 1) scaffolds demonstrated the best profile, the highest uniform porosity and connectivity, and excellent hydroscopicity, and also exhibited appropriate and controlled swelling and degradation characteristics.
32773734	5	103	theme	line	1167:1170	arg1	capacity					1120:1127	spheroid-forming capacity	1103:1127	spheroid-forming capacity of the commercially available CC cell line HCT-116	1103:1178	We analyzed the physicochemical characteristics of these scaffolds and studied cell adhesion, cell proliferation, migration, colony-forming ability, microstructure and ultrastructure, and spheroid-forming capacity of the commercially available CC cell line HCT-116 on the prepared scaffolds.
32773734	5	103	theme	line	1167:1170	arg1	proliferation					1014:1026	cell proliferation	1009:1026	cell proliferation	1009:1026	We analyzed the physicochemical characteristics of these scaffolds and studied cell adhesion, cell proliferation, migration, colony-forming ability, microstructure and ultrastructure, and spheroid-forming capacity of the commercially available CC cell line HCT-116 on the prepared scaffolds.
32773734	5	103	theme	line	1167:1170	arg1	microstructure					1064:1077	microstructure	1064:1077	microstructure	1064:1077	We analyzed the physicochemical characteristics of these scaffolds and studied cell adhesion, cell proliferation, migration, colony-forming ability, microstructure and ultrastructure, and spheroid-forming capacity of the commercially available CC cell line HCT-116 on the prepared scaffolds.
32773734	5	103	theme	line	1167:1170	arg1	ultrastructure					1083:1096	ultrastructure	1083:1096	ultrastructure	1083:1096	We analyzed the physicochemical characteristics of these scaffolds and studied cell adhesion, cell proliferation, migration, colony-forming ability, microstructure and ultrastructure, and spheroid-forming capacity of the commercially available CC cell line HCT-116 on the prepared scaffolds.
32773734	5	103	theme	line	1167:1170	arg1	migration					1029:1037	migration	1029:1037	migration	1029:1037	We analyzed the physicochemical characteristics of these scaffolds and studied cell adhesion, cell proliferation, migration, colony-forming ability, microstructure and ultrastructure, and spheroid-forming capacity of the commercially available CC cell line HCT-116 on the prepared scaffolds.
32773734	5	103	theme	line	1167:1170	arg1	adhesion					999:1006	cell adhesion	994:1006	cell adhesion	994:1006	We analyzed the physicochemical characteristics of these scaffolds and studied cell adhesion, cell proliferation, migration, colony-forming ability, microstructure and ultrastructure, and spheroid-forming capacity of the commercially available CC cell line HCT-116 on the prepared scaffolds.
32773734	5	103	theme	line	1167:1170	arg1	ability					1055:1061	colony-forming ability	1040:1061	colony-forming ability	1040:1061	We analyzed the physicochemical characteristics of these scaffolds and studied cell adhesion, cell proliferation, migration, colony-forming ability, microstructure and ultrastructure, and spheroid-forming capacity of the commercially available CC cell line HCT-116 on the prepared scaffolds.
32773734	1	104	theme	in	308:309	arg1	models					336:341	two-dimensional cell culture in vitro and in vivo cancer models	279:341	two-dimensional cell culture in vitro and in vivo cancer models	279:341	BACKGROUND Three-dimensional (3D) cell-culture scaffolds are ideal in vitro models to bridge the gap between two-dimensional cell culture in vitro and in vivo cancer models.
32773734	7	105	theme	scanning	1651:1658	arg1	microscopy					1669:1678	scanning electron microscopy	1651:1678	scanning electron microscopy	1651:1678	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	105	theme	scanning	1651:1658	arg1	assays					1526:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	106	theme	DY-554-phalloidin	1623:1639	arg1	staining					1641:1648	6-diamidino-2-phenylindole and DY-554-phalloidin staining	1592:1648	staining	1641:1648	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	106	theme	DY-554-phalloidin	1623:1639	arg1	assays					1526:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	3	107	theme	triple	590:595	arg1	scaffolds					607:615	triple composite scaffolds	590:615	triple composite scaffolds using silk fibroin (SF), chitosan (Cs), and alginate (Alg)	590:674	To improve the performance of the scaffolds for 3D cell culture of colonic carcinoma (CC) cells in vitro, we attempted to construct triple composite scaffolds using silk fibroin (SF), chitosan (Cs), and alginate (Alg).
32773734	7	108	theme	6-diamidino-2-phenylindole	1592:1617	arg1	staining					1641:1648	6-diamidino-2-phenylindole and DY-554-phalloidin staining	1592:1648	staining	1641:1648	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	7	108	theme	6-diamidino-2-phenylindole	1592:1617	arg1	assays					1526:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays	1463:1531	The adhesion, proliferation, colony-forming, and wound-healing assays, green fluorescent protein-labeled HCT116 cell imaging, 4',6-diamidino-2-phenylindole and DY-554-phalloidin staining, scanning electron microscopy, and haematoxylin and eosin staining revealed that the triple composite scaffolds of SF/CS/Alg (1: 1: 1) supported cell adhesion, proliferation, migration, colony-forming ability, and spheroid formation far better than the dual composite scaffold of SF/CS (1: 1).
32773734	4	109	theme	composite	860:868	arg1	group					908:912	a control group	898:912	a control group	898:912	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	4	109	theme	composite	860:868	arg1	scaffold					870:877	the dual composite scaffold	851:877	the dual composite scaffold of SF/Cs (1: 1)	851:893	MATERIAL AND METHODS We explored the suitability of triple composite scaffolds of SF/Cs/Alg at ratios of 1: 1: 0.5, 1: 1: 1, and 1: 1: 2 for 3D culture of CC cells, and used the dual composite scaffold of SF/Cs (1: 1) as a control group.
32773734	1	110	theme	ideal	231:235	arg1	models					246:251	ideal in vitro models	231:251	ideal in vitro models	231:251	BACKGROUND Three-dimensional (3D) cell-culture scaffolds are ideal in vitro models to bridge the gap between two-dimensional cell culture in vitro and in vivo cancer models.
32773734	1	110	theme	ideal	231:235	arg1	scaffolds					217:225	BACKGROUND Three-dimensional (3D) cell-culture scaffolds	170:225	BACKGROUND Three-dimensional (3D) cell-culture scaffolds	170:225	BACKGROUND Three-dimensional (3D) cell-culture scaffolds are ideal in vitro models to bridge the gap between two-dimensional cell culture in vitro and in vivo cancer models.
32901642	2	0	theme	-induced	630:637	arg1	hepatotoxicity					639:652	cyclophosphamide (CTX)-induced hepatotoxicity	608:652	cyclophosphamide (CTX)-induced hepatotoxicity	608:652	In the current study, the hepatoprotective effects of FAOP in mice with cyclophosphamide (CTX)-induced hepatotoxicity were investigated.
32901642	7	1	theme	SCFA	1777:1780	arg1	production					1782:1791	enhanced SCFA production	1768:1791	enhanced SCFA production than unprocessed AOP	1768:1812	Therefore, LAB NCU116 fermentation of Asparagus officinalis was practical and effective to obtain promising hepatoprotective polysaccharides, which might arise from enhanced SCFA production than unprocessed AOP.
32901642	3	2	theme	CTX-induced	722:732	arg1	damage					742:747	CTX-induced hepatic damage	722:747	CTX-induced hepatic damage	722:747	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	0	3	theme	production	187:196	arg1	modulation					117:126	modulation	117:126	modulation of glutathione homeostasis, bile acid metabolism, and SCFA production	117:196	Polysaccharides from fermented Asparagus officinalis with Lactobacillus plantarum NCU116 alleviated liver injury via modulation of glutathione homeostasis, bile acid metabolism, and SCFA production.
32901642	4	4	theme	GSH	1031:1033	arg1	biosynthesis					1035:1046	GSH biosynthesis	1031:1046	GSH biosynthesis	1031:1046	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	1	5	theme	unprocessed	479:489	arg1	polysaccharides					513:527	unprocessed Asparagus officinalis polysaccharides	479:527	unprocessed Asparagus officinalis polysaccharides (AOP)	479:533	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	1	5	theme	unprocessed	479:489	arg1	AOP					530:532	AOP	530:532	AOP	530:532	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	6	6	theme	FAOP	1481:1484	arg1	superiority					1466:1476	the hepatoprotective superiority	1445:1476	the hepatoprotective superiority of FAOP	1445:1484	Furthermore, serum metabolomics study validated the hepatoprotective superiority of FAOP than AOP with evidence from variations in bile acid compositions and the construction of related metabolic pathways.
32901642	7	7	theme	NCU116	1618:1623	arg1	fermentation					1625:1636	LAB NCU116 fermentation	1614:1636	LAB NCU116 fermentation of Asparagus officinalis	1614:1661	Therefore, LAB NCU116 fermentation of Asparagus officinalis was practical and effective to obtain promising hepatoprotective polysaccharides, which might arise from enhanced SCFA production than unprocessed AOP.
32901642	4	8	theme	acid	1094:1097	arg1	receptors					1099:1107	bile acid receptors	1089:1107	bile acid receptors (FXR and SHP)	1089:1121	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	4	8	theme	acid	1094:1097	arg1	SHP					1118:1120	SHP	1118:1120	SHP	1118:1120	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	4	8	theme	acid	1094:1097	arg1	FXR					1110:1112	FXR	1110:1112	FXR	1110:1112	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	1	9	theme	officinalis	501:511	arg1	polysaccharides					513:527	unprocessed Asparagus officinalis polysaccharides	479:527	unprocessed Asparagus officinalis polysaccharides (AOP)	479:533	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	1	9	theme	officinalis	501:511	arg1	AOP					530:532	AOP	530:532	AOP	530:532	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	5	10	theme	receptors	1330:1338	arg1	upregulation					1276:1287	the upregulation	1272:1287	the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production	1272:1394	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	5	10	theme	receptors	1330:1338	arg1	production					1385:1394	intestinal SCFA production	1369:1394	the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production	1272:1394	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	1	11	from	changes	333:339	arg1	properties					360:369	physicochemical properties	344:369	physicochemical properties such as monosaccharide composition and molecular weight	344:425	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	1	11	from	changes	333:339	arg1	composition					394:404	monosaccharide composition	379:404	monosaccharide composition	379:404	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	1	11	from	changes	333:339	arg1	weight					420:425	molecular weight	410:425	molecular weight	410:425	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	2	12	theme	FAOP	590:593	arg1	effects					579:585	the hepatoprotective effects	558:585	the hepatoprotective effects of FAOP in mice with cyclophosphamide (CTX)-induced hepatotoxicity	558:652	In the current study, the hepatoprotective effects of FAOP in mice with cyclophosphamide (CTX)-induced hepatotoxicity were investigated.
32901642	7	13	theme	hepatoprotective	1711:1726	arg1	polysaccharides					1728:1742	promising hepatoprotective polysaccharides	1701:1742	promising hepatoprotective polysaccharides	1701:1742	Therefore, LAB NCU116 fermentation of Asparagus officinalis was practical and effective to obtain promising hepatoprotective polysaccharides, which might arise from enhanced SCFA production than unprocessed AOP.
32901642	4	14	theme	expression	1069:1078	arg1	level					1080:1084	the expression level	1065:1084	the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1)	1065:1189	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	3	15	theme	biochemical	782:792	arg1	LDH					821:823	LDH	821:823	LDH	821:823	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	3	15	theme	biochemical	782:792	arg1	AST					808:810	AST	808:810	AST	808:810	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	3	15	theme	biochemical	782:792	arg1	ALT					803:805	ALT	803:805	ALT	803:805	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	3	15	theme	biochemical	782:792	arg1	markers					794:800	hepatic biochemical markers	774:800	hepatic biochemical markers (ALT, AST, AKP and LDH)	774:824	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	3	15	theme	biochemical	782:792	arg1	AKP					813:815	AKP	813:815	AKP	813:815	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	0	16	theme	homeostasis	143:153	arg1	modulation					117:126	modulation	117:126	modulation of glutathione homeostasis, bile acid metabolism, and SCFA production	117:196	Polysaccharides from fermented Asparagus officinalis with Lactobacillus plantarum NCU116 alleviated liver injury via modulation of glutathione homeostasis, bile acid metabolism, and SCFA production.
32901642	1	17	theme	molecular	410:418	arg1	weight					420:425	molecular weight	410:425	molecular weight	410:425	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	0	18	theme	bile	156:159	arg1	metabolism					166:175	bile acid metabolism	156:175	bile acid metabolism	156:175	Polysaccharides from fermented Asparagus officinalis with Lactobacillus plantarum NCU116 alleviated liver injury via modulation of glutathione homeostasis, bile acid metabolism, and SCFA production.
32901642	6	19	theme	bile	1528:1531	arg1	compositions					1538:1549	bile acid compositions	1528:1549	bile acid compositions	1528:1549	Furthermore, serum metabolomics study validated the hepatoprotective superiority of FAOP than AOP with evidence from variations in bile acid compositions and the construction of related metabolic pathways.
32901642	4	20	theme	key	1127:1129	arg1	enzymes					1131:1137	key enzymes	1127:1137	key enzymes	1127:1137	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	0	21	theme	metabolism	166:175	arg1	modulation					117:126	modulation	117:126	modulation of glutathione homeostasis, bile acid metabolism, and SCFA production	117:196	Polysaccharides from fermented Asparagus officinalis with Lactobacillus plantarum NCU116 alleviated liver injury via modulation of glutathione homeostasis, bile acid metabolism, and SCFA production.
32901642	5	22	theme	homeostasis	1217:1227	arg1	Modulation					1192:1201	Modulation	1192:1201	Modulation of disordered homeostasis of bile acids by FAOP	1192:1249	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	1	23	theme	enhanced	449:456	arg1	activity					465:472	their enhanced immune activity	443:472	their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP)	443:533	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	6	24	theme	pathways	1593:1600	arg1	evidence					1500:1507	evidence	1500:1507	evidence from variations in bile acid compositions	1500:1549	Furthermore, serum metabolomics study validated the hepatoprotective superiority of FAOP than AOP with evidence from variations in bile acid compositions and the construction of related metabolic pathways.
32901642	6	24	theme	pathways	1593:1600	arg1	construction					1559:1570	the construction	1555:1570	the construction of related metabolic pathways	1555:1600	Furthermore, serum metabolomics study validated the hepatoprotective superiority of FAOP than AOP with evidence from variations in bile acid compositions and the construction of related metabolic pathways.
32901642	5	25	theme	bile	1232:1235	arg1	acids					1237:1241	bile acids	1232:1241	bile acids	1232:1241	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	4	26	dep	receptors	1099:1107	arg1	receptors					1099:1107	bile acid receptors	1089:1107	bile acid receptors (FXR and SHP)	1089:1121	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	4	26	dep	receptors	1099:1107	arg1	SHP					1118:1120	SHP	1118:1120	SHP	1118:1120	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	4	26	dep	receptors	1099:1107	arg1	FXR					1110:1112	FXR	1110:1112	FXR	1110:1112	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	5	27	dep	receptors	1330:1338	arg1	receptors					1330:1338	hepatic short chain fatty acid (SCFA) receptors	1292:1338	hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A	1292:1356	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	5	27	dep	receptors	1330:1338	arg1	GPR109A					1350:1356	GPR109A	1350:1356	GPR109A	1350:1356	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	5	27	dep	receptors	1330:1338	arg1	GPR41					1340:1344	GPR41	1340:1344	GPR41	1340:1344	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	0	28	theme	liver	100:104	arg1	injury					106:111	liver injury	100:111	liver injury	100:111	Polysaccharides from fermented Asparagus officinalis with Lactobacillus plantarum NCU116 alleviated liver injury via modulation of glutathione homeostasis, bile acid metabolism, and SCFA production.
32901642	6	29	theme	metabolic	1583:1591	arg1	pathways					1593:1600	related metabolic pathways	1575:1600	related metabolic pathways	1575:1600	Furthermore, serum metabolomics study validated the hepatoprotective superiority of FAOP than AOP with evidence from variations in bile acid compositions and the construction of related metabolic pathways.
32901642	7	30	theme	Asparagus	1641:1649	arg1	officinalis					1651:1661	Asparagus officinalis	1641:1661	Asparagus officinalis	1641:1661	Therefore, LAB NCU116 fermentation of Asparagus officinalis was practical and effective to obtain promising hepatoprotective polysaccharides, which might arise from enhanced SCFA production than unprocessed AOP.
32901642	3	31	theme	pro-inflammatory	830:845	arg1	cytokines					847:855	pro-inflammatory cytokines	830:855	pro-inflammatory cytokines (TNF-α and IL-1β)	830:873	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	3	31	theme	pro-inflammatory	830:845	arg1	IL-1β					868:872	IL-1β	868:872	IL-1β	868:872	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	3	31	theme	pro-inflammatory	830:845	arg1	TNF-α					858:862	TNF-α	858:862	TNF-α	858:862	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	7	32	theme	LAB	1614:1616	arg1	fermentation					1625:1636	LAB NCU116 fermentation	1614:1636	LAB NCU116 fermentation of Asparagus officinalis	1614:1661	Therefore, LAB NCU116 fermentation of Asparagus officinalis was practical and effective to obtain promising hepatoprotective polysaccharides, which might arise from enhanced SCFA production than unprocessed AOP.
32901642	0	33	from	officinalis	41:51	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from fermented Asparagus officinalis with Lactobacillus plantarum NCU116	0:87	Polysaccharides from fermented Asparagus officinalis with Lactobacillus plantarum NCU116 alleviated liver injury via modulation of glutathione homeostasis, bile acid metabolism, and SCFA production.
32901642	5	34	theme	SCFA	1380:1383	arg1	production					1385:1394	intestinal SCFA production	1369:1394	the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production	1272:1394	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	4	35	theme	acid	1147:1150	arg1	synthesis					1152:1160	bile acid synthesis	1142:1160	bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1)	1142:1189	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	3	36	dep	reinforcement	886:898	arg1	T-AOC					924:928	T-AOC	924:928	T-AOC	924:928	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	0	37	theme	fermented	21:29	arg1	officinalis					41:51	fermented Asparagus officinalis	21:51	fermented Asparagus officinalis	21:51	Polysaccharides from fermented Asparagus officinalis with Lactobacillus plantarum NCU116 alleviated liver injury via modulation of glutathione homeostasis, bile acid metabolism, and SCFA production.
32901642	5	38	theme	fatty	1312:1316	arg1	SCFA					1324:1327	SCFA	1324:1327	SCFA	1324:1327	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	5	38	theme	fatty	1312:1316	arg1	acid					1318:1321	hepatic short chain fatty acid	1292:1321	hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A	1292:1356	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	0	39	theme	Lactobacillus	58:70	arg1	NCU116					82:87	Lactobacillus plantarum NCU116	58:87	Lactobacillus plantarum NCU116	58:87	Polysaccharides from fermented Asparagus officinalis with Lactobacillus plantarum NCU116 alleviated liver injury via modulation of glutathione homeostasis, bile acid metabolism, and SCFA production.
32901642	1	40	theme	substantial	321:331	arg1	changes					333:339	substantial changes	321:339	substantial changes in physicochemical properties such as monosaccharide composition and molecular weight	321:425	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	7	41	theme	unprocessed	1798:1808	arg1	AOP					1810:1812	unprocessed AOP	1798:1812	unprocessed AOP	1798:1812	Therefore, LAB NCU116 fermentation of Asparagus officinalis was practical and effective to obtain promising hepatoprotective polysaccharides, which might arise from enhanced SCFA production than unprocessed AOP.
32901642	0	42	with	Polysaccharides	0:14	arg1	NCU116					82:87	Lactobacillus plantarum NCU116	58:87	Lactobacillus plantarum NCU116	58:87	Polysaccharides from fermented Asparagus officinalis with Lactobacillus plantarum NCU116 alleviated liver injury via modulation of glutathione homeostasis, bile acid metabolism, and SCFA production.
32901642	1	43	dep	bacteria	211:218	arg1	strain					220:225	strain	220:225	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP)	199:293	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	1	43	dep	bacteria	211:218	arg1	FAOP					289:292	FAOP	289:292	FAOP	289:292	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	1	43	dep	bacteria	211:218	arg1	polysaccharides					272:286	NCU116 fermented Asparagus officinalis polysaccharides	233:286	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP)	199:293	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	1	44	theme	fermented	240:248	arg1	polysaccharides					272:286	NCU116 fermented Asparagus officinalis polysaccharides	233:286	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP)	199:293	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	1	44	theme	fermented	240:248	arg1	FAOP					289:292	FAOP	289:292	FAOP	289:292	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	7	45	theme	enhanced	1768:1775	arg1	production					1782:1791	enhanced SCFA production	1768:1791	enhanced SCFA production than unprocessed AOP	1768:1812	Therefore, LAB NCU116 fermentation of Asparagus officinalis was practical and effective to obtain promising hepatoprotective polysaccharides, which might arise from enhanced SCFA production than unprocessed AOP.
32901642	5	46	theme	short	1300:1304	arg1	SCFA					1324:1327	SCFA	1324:1327	SCFA	1324:1327	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	5	46	theme	short	1300:1304	arg1	acid					1318:1321	hepatic short chain fatty acid	1292:1321	hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A	1292:1356	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	1	47	theme	officinalis	260:270	arg1	polysaccharides					272:286	NCU116 fermented Asparagus officinalis polysaccharides	233:286	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP)	199:293	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	1	47	theme	officinalis	260:270	arg1	FAOP					289:292	FAOP	289:292	FAOP	289:292	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	3	48	dep	markers	794:800	arg1	AST					808:810	AST	808:810	AST	808:810	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	3	48	dep	markers	794:800	arg1	ALT					803:805	ALT	803:805	ALT	803:805	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	3	48	dep	markers	794:800	arg1	markers					794:800	hepatic biochemical markers	774:800	hepatic biochemical markers (ALT, AST, AKP and LDH)	774:824	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	3	48	dep	markers	794:800	arg1	LDH					821:823	LDH	821:823	LDH	821:823	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	3	48	dep	markers	794:800	arg1	AKP					813:815	AKP	813:815	AKP	813:815	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	3	49	theme	hepatic	734:740	arg1	damage					742:747	CTX-induced hepatic damage	722:747	CTX-induced hepatic damage	722:747	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	4	50	theme	receptors	1099:1107	arg1	level					1080:1084	the expression level	1065:1084	the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1)	1065:1189	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	3	51	theme	hepatic	774:780	arg1	LDH					821:823	LDH	821:823	LDH	821:823	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	3	51	theme	hepatic	774:780	arg1	AST					808:810	AST	808:810	AST	808:810	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	3	51	theme	hepatic	774:780	arg1	ALT					803:805	ALT	803:805	ALT	803:805	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	3	51	theme	hepatic	774:780	arg1	markers					794:800	hepatic biochemical markers	774:800	hepatic biochemical markers (ALT, AST, AKP and LDH)	774:824	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	3	51	theme	hepatic	774:780	arg1	AKP					813:815	AKP	813:815	AKP	813:815	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	1	52	theme	Asparagus	491:499	arg1	polysaccharides					513:527	unprocessed Asparagus officinalis polysaccharides	479:527	unprocessed Asparagus officinalis polysaccharides (AOP)	479:533	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	1	52	theme	Asparagus	491:499	arg1	AOP					530:532	AOP	530:532	AOP	530:532	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	6	53	theme	hepatoprotective	1449:1464	arg1	superiority					1466:1476	the hepatoprotective superiority	1445:1476	the hepatoprotective superiority of FAOP	1445:1484	Furthermore, serum metabolomics study validated the hepatoprotective superiority of FAOP than AOP with evidence from variations in bile acid compositions and the construction of related metabolic pathways.
32901642	1	54	theme	Lactic	199:204	arg1	bacteria					211:218	Lactic acid bacteria	199:218	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP)	199:293	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	1	54	theme	Lactic	199:204	arg1	LAB					228:230	LAB	228:230	LAB	228:230	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	7	55	theme	promising	1701:1709	arg1	polysaccharides					1728:1742	promising hepatoprotective polysaccharides	1701:1742	promising hepatoprotective polysaccharides	1701:1742	Therefore, LAB NCU116 fermentation of Asparagus officinalis was practical and effective to obtain promising hepatoprotective polysaccharides, which might arise from enhanced SCFA production than unprocessed AOP.
32901642	4	56	theme	bile	1089:1092	arg1	receptors					1099:1107	bile acid receptors	1089:1107	bile acid receptors (FXR and SHP)	1089:1121	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	4	56	theme	bile	1089:1092	arg1	SHP					1118:1120	SHP	1118:1120	SHP	1118:1120	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	4	56	theme	bile	1089:1092	arg1	FXR					1110:1112	FXR	1110:1112	FXR	1110:1112	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	3	57	theme	markers	794:800	arg1	inhibition					760:769	inhibition	760:769	inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA)	760:948	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	3	57	theme	markers	794:800	arg1	reinforcement					886:898	reinforcement	886:898	inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA)	760:948	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	0	58	theme	acid	161:164	arg1	metabolism					166:175	bile acid metabolism	156:175	bile acid metabolism	156:175	Polysaccharides from fermented Asparagus officinalis with Lactobacillus plantarum NCU116 alleviated liver injury via modulation of glutathione homeostasis, bile acid metabolism, and SCFA production.
32901642	1	59	theme	acid	206:209	arg1	bacteria					211:218	Lactic acid bacteria	199:218	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP)	199:293	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	1	59	theme	acid	206:209	arg1	LAB					228:230	LAB	228:230	LAB	228:230	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	4	60	from	level	1080:1084	arg1	synthesis					1152:1160	bile acid synthesis	1142:1160	bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1)	1142:1189	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	2	61	theme	hepatoprotective	562:577	arg1	effects					579:585	the hepatoprotective effects	558:585	the hepatoprotective effects of FAOP in mice with cyclophosphamide (CTX)-induced hepatotoxicity	558:652	In the current study, the hepatoprotective effects of FAOP in mice with cyclophosphamide (CTX)-induced hepatotoxicity were investigated.
32901642	4	62	theme	enzymes	1131:1137	arg1	level					1080:1084	the expression level	1065:1084	the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1)	1065:1189	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	6	63	from	variations	1514:1523	arg1	evidence					1500:1507	evidence	1500:1507	evidence from variations in bile acid compositions	1500:1549	Furthermore, serum metabolomics study validated the hepatoprotective superiority of FAOP than AOP with evidence from variations in bile acid compositions and the construction of related metabolic pathways.
32901642	6	63	from	variations	1514:1523	arg1	compositions					1538:1549	bile acid compositions	1528:1549	bile acid compositions	1528:1549	Furthermore, serum metabolomics study validated the hepatoprotective superiority of FAOP than AOP with evidence from variations in bile acid compositions and the construction of related metabolic pathways.
32901642	6	63	from	variations	1514:1523	arg1	construction					1559:1570	the construction	1555:1570	the construction of related metabolic pathways	1555:1600	Furthermore, serum metabolomics study validated the hepatoprotective superiority of FAOP than AOP with evidence from variations in bile acid compositions and the construction of related metabolic pathways.
32901642	4	64	theme	bile	1142:1145	arg1	synthesis					1152:1160	bile acid synthesis	1142:1160	bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1)	1142:1189	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	5	65	theme	disordered	1206:1215	arg1	homeostasis					1217:1227	disordered homeostasis	1206:1227	disordered homeostasis of bile acids	1206:1241	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	0	66	theme	SCFA	182:185	arg1	production					187:196	SCFA production	182:196	SCFA production	182:196	Polysaccharides from fermented Asparagus officinalis with Lactobacillus plantarum NCU116 alleviated liver injury via modulation of glutathione homeostasis, bile acid metabolism, and SCFA production.
32901642	3	67	dep	T-AOC	924:928	arg1	MDA					945:947	MDA	945:947	MDA	945:947	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	3	67	dep	T-AOC	924:928	arg1	SOD					931:933	SOD	931:933	SOD	931:933	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	3	67	dep	T-AOC	924:928	arg1	CAT					936:938	CAT	936:938	CAT	936:938	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	1	68	theme	immune	458:463	arg1	activity					465:472	their enhanced immune activity	443:472	their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP)	443:533	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	1	69	theme	physicochemical	344:358	arg1	properties					360:369	physicochemical properties	344:369	physicochemical properties such as monosaccharide composition and molecular weight	344:425	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	1	69	theme	physicochemical	344:358	arg1	composition					394:404	monosaccharide composition	379:404	monosaccharide composition	379:404	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	1	69	theme	physicochemical	344:358	arg1	weight					420:425	molecular weight	410:425	molecular weight	410:425	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	6	70	theme	related	1575:1581	arg1	pathways					1593:1600	related metabolic pathways	1575:1600	related metabolic pathways	1575:1600	Furthermore, serum metabolomics study validated the hepatoprotective superiority of FAOP than AOP with evidence from variations in bile acid compositions and the construction of related metabolic pathways.
32901642	7	71	theme	officinalis	1651:1661	arg1	fermentation					1625:1636	LAB NCU116 fermentation	1614:1636	LAB NCU116 fermentation of Asparagus officinalis	1614:1661	Therefore, LAB NCU116 fermentation of Asparagus officinalis was practical and effective to obtain promising hepatoprotective polysaccharides, which might arise from enhanced SCFA production than unprocessed AOP.
32901642	3	72	theme	cytokines	847:855	arg1	inhibition					760:769	inhibition	760:769	inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA)	760:948	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	3	72	theme	cytokines	847:855	arg1	reinforcement					886:898	reinforcement	886:898	inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA)	760:948	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	0	73	theme	glutathione	131:141	arg1	homeostasis					143:153	glutathione homeostasis	131:153	glutathione homeostasis	131:153	Polysaccharides from fermented Asparagus officinalis with Lactobacillus plantarum NCU116 alleviated liver injury via modulation of glutathione homeostasis, bile acid metabolism, and SCFA production.
32901642	1	74	theme	monosaccharide	379:392	arg1	composition					394:404	monosaccharide composition	379:404	monosaccharide composition	379:404	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	2	75	theme	current	543:549	arg1	study					551:555	the current study	539:555	the current study	539:555	In the current study, the hepatoprotective effects of FAOP in mice with cyclophosphamide (CTX)-induced hepatotoxicity were investigated.
32901642	5	76	theme	intestinal	1369:1378	arg1	production					1385:1394	intestinal SCFA production	1369:1394	the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production	1272:1394	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	3	77	theme	systems	915:921	arg1	inhibition					760:769	inhibition	760:769	inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA)	760:948	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	3	77	theme	systems	915:921	arg1	reinforcement					886:898	reinforcement	886:898	inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA)	760:948	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	5	78	theme	acids	1237:1241	arg1	homeostasis					1217:1227	disordered homeostasis	1206:1227	disordered homeostasis of bile acids	1206:1241	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	0	79	theme	Asparagus	31:39	arg1	officinalis					41:51	fermented Asparagus officinalis	21:51	fermented Asparagus officinalis	21:51	Polysaccharides from fermented Asparagus officinalis with Lactobacillus plantarum NCU116 alleviated liver injury via modulation of glutathione homeostasis, bile acid metabolism, and SCFA production.
32901642	6	80	theme	acid	1533:1536	arg1	compositions					1538:1549	bile acid compositions	1528:1549	bile acid compositions	1528:1549	Furthermore, serum metabolomics study validated the hepatoprotective superiority of FAOP than AOP with evidence from variations in bile acid compositions and the construction of related metabolic pathways.
32901642	4	81	theme	superior	997:1004	arg1	performance					1006:1016	superior performance	997:1016	superior performance	997:1016	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	6	82	theme	metabolomics	1416:1427	arg1	study					1429:1433	serum metabolomics study	1410:1433	serum metabolomics study	1410:1433	Furthermore, serum metabolomics study validated the hepatoprotective superiority of FAOP than AOP with evidence from variations in bile acid compositions and the construction of related metabolic pathways.
32901642	5	83	theme	chain	1306:1310	arg1	SCFA					1324:1327	SCFA	1324:1327	SCFA	1324:1327	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	5	83	theme	chain	1306:1310	arg1	acid					1318:1321	hepatic short chain fatty acid	1292:1321	hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A	1292:1356	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	0	84	theme	plantarum	72:80	arg1	NCU116					82:87	Lactobacillus plantarum NCU116	58:87	Lactobacillus plantarum NCU116	58:87	Polysaccharides from fermented Asparagus officinalis with Lactobacillus plantarum NCU116 alleviated liver injury via modulation of glutathione homeostasis, bile acid metabolism, and SCFA production.
32901642	5	85	theme	acid	1318:1321	arg1	receptors					1330:1338	hepatic short chain fatty acid (SCFA) receptors	1292:1338	hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A	1292:1356	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	5	85	theme	acid	1318:1321	arg1	GPR109A					1350:1356	GPR109A	1350:1356	GPR109A	1350:1356	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	5	85	theme	acid	1318:1321	arg1	GPR41					1340:1344	GPR41	1340:1344	GPR41	1340:1344	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	6	86	theme	serum	1410:1414	arg1	study					1429:1433	serum metabolomics study	1410:1433	serum metabolomics study	1410:1433	Furthermore, serum metabolomics study validated the hepatoprotective superiority of FAOP than AOP with evidence from variations in bile acid compositions and the construction of related metabolic pathways.
32901642	2	87	from	effects	579:585	arg1	mice					598:601	mice	598:601	mice with cyclophosphamide (CTX)-induced hepatotoxicity	598:652	In the current study, the hepatoprotective effects of FAOP in mice with cyclophosphamide (CTX)-induced hepatotoxicity were investigated.
32901642	4	88	dep	synthesis	1152:1160	arg1	CYP8B1					1171:1176	CYP8B1	1171:1176	CYP8B1	1171:1176	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	4	88	dep	synthesis	1152:1160	arg1	CYP7A1					1163:1168	CYP7A1	1163:1168	CYP7A1	1163:1168	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	4	88	dep	synthesis	1152:1160	arg1	CYP27A1					1182:1188	CYP27A1	1182:1188	CYP27A1	1182:1188	In particular, compared with AOP, FAOP showed superior performance by promoting GSH biosynthesis, and normalizing the expression level of bile acid receptors (FXR and SHP) and key enzymes in bile acid synthesis (CYP7A1, CYP8B1 and CYP27A1).
32901642	3	89	theme	antioxidant	903:913	arg1	systems					915:921	antioxidant systems	903:921	antioxidant systems	903:921	FAOP were more effective than AOP in alleviating CTX-induced hepatic damage, including inhibition of hepatic biochemical markers (ALT, AST, AKP and LDH) and pro-inflammatory cytokines (TNF-α and IL-1β) as well as reinforcement of antioxidant systems (T-AOC, SOD, CAT, and MDA).
32901642	5	90	theme	hepatic	1292:1298	arg1	SCFA					1324:1327	SCFA	1324:1327	SCFA	1324:1327	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	5	90	theme	hepatic	1292:1298	arg1	acid					1318:1321	hepatic short chain fatty acid	1292:1321	hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A	1292:1356	Modulation of disordered homeostasis of bile acids by FAOP can be attributed to the upregulation of hepatic short chain fatty acid (SCFA) receptors GPR41 and GPR109A as well as intestinal SCFA production.
32901642	2	91	with	mice	598:601	arg1	hepatotoxicity					639:652	cyclophosphamide (CTX)-induced hepatotoxicity	608:652	cyclophosphamide (CTX)-induced hepatotoxicity	608:652	In the current study, the hepatoprotective effects of FAOP in mice with cyclophosphamide (CTX)-induced hepatotoxicity were investigated.
32901642	1	92	theme	Asparagus	250:258	arg1	polysaccharides					272:286	NCU116 fermented Asparagus officinalis polysaccharides	233:286	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP)	199:293	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
32901642	1	92	theme	Asparagus	250:258	arg1	FAOP					289:292	FAOP	289:292	FAOP	289:292	Lactic acid bacteria strain (LAB) NCU116 fermented Asparagus officinalis polysaccharides (FAOP) have been proven to cause substantial changes in physicochemical properties such as monosaccharide composition and molecular weight, accounting for their enhanced immune activity than unprocessed Asparagus officinalis polysaccharides (AOP).
34389396	4	0	theme	Chitosan-Lanthanide	764:782	arg1	Ch-LnO					792:797	Ch-LnO	792:797	Ch-LnO	792:797	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
34389396	4	0	theme	Chitosan-Lanthanide	764:782	arg1	oxides					784:789	different Chitosan-Lanthanide oxides	754:789	different Chitosan-Lanthanide oxides (Ch-LnO)	754:798	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
34389396	2	1	theme	optical	289:295	arg1	function					346:353	a function	344:353	a function of the ion size of the lanthanide element	344:395	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	2	1	theme	optical	289:295	arg1	properties					317:326	its optical and thermodynamical properties	285:326	its optical and thermodynamical properties	285:326	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	2	1	theme	optical	289:295	arg1	masses					412:417	its atomic masses	401:417	its atomic masses	401:417	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	1	2	from	investigation	38:50	arg1	properties					82:91	the optical and ultrasonic properties	55:91	the optical and ultrasonic properties of chitosan-lanthanide composites	55:125	An investigation on the optical and ultrasonic properties of chitosan-lanthanide composites.
34389396	2	3	theme	atomic	405:410	arg1	properties					317:326	its optical and thermodynamical properties	285:326	its optical and thermodynamical properties	285:326	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	2	3	theme	atomic	405:410	arg1	masses					412:417	its atomic masses	401:417	its atomic masses	401:417	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	4	4	theme	different	754:762	arg1	Ch-LnO					792:797	Ch-LnO	792:797	Ch-LnO	792:797	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
34389396	4	4	theme	different	754:762	arg1	oxides					784:789	different Chitosan-Lanthanide oxides	754:789	different Chitosan-Lanthanide oxides (Ch-LnO)	754:798	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
34389396	3	5	theme	refractive	429:438	arg1	measurement					446:456	the refractive index measurement	425:456	the refractive index measurement	425:456	From the refractive index measurement, the space-filling factor and polarizability have been obtained.
34389396	6	6	theme	properties	887:896	arg1	variation					861:869	The variation	857:869	The variation of all the said properties with ion size	857:910	The variation of all the said properties with ion size is opposite to that of atomic mass due to lanthanide contraction.
34389396	6	6	theme	properties	887:896	arg1	opposite					915:922	opposite	915:922	opposite	915:922	The variation of all the said properties with ion size is opposite to that of atomic mass due to lanthanide contraction.
34389396	3	7	theme	space-filling	463:475	arg1	factor					477:482	the space-filling factor	459:482	the space-filling factor	459:482	From the refractive index measurement, the space-filling factor and polarizability have been obtained.
34389396	6	8	theme	said	882:885	arg1	properties					887:896	all the said properties	874:896	all the said properties	874:896	The variation of all the said properties with ion size is opposite to that of atomic mass due to lanthanide contraction.
34389396	4	9	theme	adiabatic	645:653	arg1	Ks					669:670	Ks	669:670	Ks	669:670	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
34389396	4	9	theme	adiabatic	645:653	arg1	modulus					660:666	adiabatic bulk modulus	645:666	adiabatic bulk modulus (Ks)	645:671	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
34389396	4	10	theme	ultrasonic	564:573	arg1	rs					640:641	rs	640:641	rs	640:641	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
34389396	4	10	theme	ultrasonic	564:573	arg1	velocity					575:582	ultrasonic velocity	564:582	ultrasonic velocity	564:582	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
34389396	6	11	theme	lanthanide	954:963	arg1	contraction					965:975	lanthanide contraction	954:975	lanthanide contraction	954:975	The variation of all the said properties with ion size is opposite to that of atomic mass due to lanthanide contraction.
34389396	7	12	theme	detailed	1023:1030	arg1	manner					1032:1037	a detailed manner	1021:1037	a detailed manner	1021:1037	The results are presented and discussed in a detailed manner.
34389396	4	13	theme	derived	592:598	arg1	quantities					600:609	its derived quantities	588:609	its derived quantities such as relaxation strength	588:637	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
34389396	4	13	theme	derived	592:598	arg1	strength					630:637	relaxation strength	619:637	relaxation strength	619:637	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
34389396	5	14	theme	different	839:847	arg1	Ch-LnO					849:854	different Ch-LnO	839:854	different Ch-LnO	839:854	FTIR studies confirm the formation of different Ch-LnO.
34389396	2	15	theme	size	366:369	arg1	function					346:353	a function	344:353	a function of the ion size of the lanthanide element	344:395	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	2	15	theme	size	366:369	arg1	properties					317:326	its optical and thermodynamical properties	285:326	its optical and thermodynamical properties	285:326	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	2	15	theme	size	366:369	arg1	masses					412:417	its atomic masses	401:417	its atomic masses	401:417	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	6	16	with	variation	861:869	arg1	size					907:910	ion size	903:910	ion size	903:910	The variation of all the said properties with ion size is opposite to that of atomic mass due to lanthanide contraction.
34389396	1	17	theme	optical	59:65	arg1	properties					82:91	the optical and ultrasonic properties	55:91	the optical and ultrasonic properties of chitosan-lanthanide composites	55:125	An investigation on the optical and ultrasonic properties of chitosan-lanthanide composites.
34389396	6	18	theme	atomic	935:940	arg1	mass					942:945	atomic mass	935:945	atomic mass due to lanthanide contraction	935:975	The variation of all the said properties with ion size is opposite to that of atomic mass due to lanthanide contraction.
34389396	2	19	theme	ion	362:364	arg1	size					366:369	the ion size	358:369	the ion size of the lanthanide element	358:395	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	5	20	theme	FTIR	801:804	arg1	studies					806:812	FTIR studies	801:812	FTIR studies	801:812	FTIR studies confirm the formation of different Ch-LnO.
34389396	3	21	theme	index	440:444	arg1	measurement					446:456	the refractive index measurement	425:456	the refractive index measurement	425:456	From the refractive index measurement, the space-filling factor and polarizability have been obtained.
34389396	1	22	theme	composites	116:125	arg1	properties					82:91	the optical and ultrasonic properties	55:91	the optical and ultrasonic properties of chitosan-lanthanide composites	55:125	An investigation on the optical and ultrasonic properties of chitosan-lanthanide composites.
34389396	0	23	theme	mass	9:12	arg1	"					13:13	mass"	9:13	mass"	9:13	"Size or mass" which plays a role?
34389396	2	24	theme	element	389:395	arg1	size					366:369	the ion size	358:369	the ion size of the lanthanide element	358:395	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	2	25	theme	present	136:142	arg1	exploration					144:154	this present exploration	131:154	this present exploration	131:154	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	1	26	theme	ultrasonic	71:80	arg1	properties					82:91	the optical and ultrasonic properties	55:91	the optical and ultrasonic properties of chitosan-lanthanide composites	55:125	An investigation on the optical and ultrasonic properties of chitosan-lanthanide composites.
34389396	2	27	theme	lanthanide	378:387	arg1	element					389:395	the lanthanide element	374:395	the lanthanide element	374:395	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	2	28	theme	lanthanide	187:196	arg1	Nd2O3					219:223	Nd2O3	219:223	Nd2O3	219:223	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	2	28	theme	lanthanide	187:196	arg1	oxides					198:203	different lanthanide oxides	177:203	different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3	177:261	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	2	28	theme	lanthanide	187:196	arg1	Ho2O3					257:261	Ho2O3	257:261	Ho2O3	257:261	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	2	28	theme	lanthanide	187:196	arg1	Eu2O3					233:237	Eu2O3	233:237	Eu2O3	233:237	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	2	28	theme	lanthanide	187:196	arg1	Dy2O3					247:251	Dy2O3	247:251	Dy2O3	247:251	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	4	29	link	derived	592:598	arg1	quantities					600:609	its derived quantities	588:609	its derived quantities such as relaxation strength	588:637	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
34389396	4	29	link	derived	592:598	arg1	strength					630:637	relaxation strength	619:637	relaxation strength	619:637	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
34389396	2	30	theme	different	177:185	arg1	Nd2O3					219:223	Nd2O3	219:223	Nd2O3	219:223	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	2	30	theme	different	177:185	arg1	oxides					198:203	different lanthanide oxides	177:203	different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3	177:261	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	2	30	theme	different	177:185	arg1	Ho2O3					257:261	Ho2O3	257:261	Ho2O3	257:261	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	2	30	theme	different	177:185	arg1	Eu2O3					233:237	Eu2O3	233:237	Eu2O3	233:237	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	2	30	theme	different	177:185	arg1	Dy2O3					247:251	Dy2O3	247:251	Dy2O3	247:251	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	4	31	theme	bulk	655:658	arg1	Ks					669:670	Ks	669:670	Ks	669:670	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
34389396	4	31	theme	bulk	655:658	arg1	modulus					660:666	adiabatic bulk modulus	645:666	adiabatic bulk modulus (Ks)	645:671	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
34389396	5	32	theme	Ch-LnO	849:854	arg1	formation					826:834	the formation	822:834	the formation of different Ch-LnO	822:854	FTIR studies confirm the formation of different Ch-LnO.
34389396	4	33	theme	ultrasonic	542:551	arg1	waves					553:557	ultrasonic waves	542:557	ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β)	542:729	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
34389396	4	34	theme	acoustic	674:681	arg1	Z					694:694	Z	694:694	Z	694:694	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
34389396	4	34	theme	acoustic	674:681	arg1	impedance					683:691	acoustic impedance	674:691	acoustic impedance (Z)	674:695	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
34389396	1	35	theme	chitosan-lanthanide	96:114	arg1	composites					116:125	chitosan-lanthanide composites	96:125	chitosan-lanthanide composites	96:125	An investigation on the optical and ultrasonic properties of chitosan-lanthanide composites.
34389396	4	36	theme	adiabatic	701:709	arg1	β					728:728	β	728:728	β	728:728	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
34389396	4	36	theme	adiabatic	701:709	arg1	compressibility					711:725	adiabatic compressibility	701:725	adiabatic compressibility (β)	701:729	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
34389396	2	37	theme	thermodynamical	301:315	arg1	function					346:353	a function	344:353	a function of the ion size of the lanthanide element	344:395	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	2	37	theme	thermodynamical	301:315	arg1	properties					317:326	its optical and thermodynamical properties	285:326	its optical and thermodynamical properties	285:326	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	2	37	theme	thermodynamical	301:315	arg1	masses					412:417	its atomic masses	401:417	its atomic masses	401:417	In this present exploration, chitosan doped with different lanthanide oxides such as CeO2, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Dy2O3 and Ho2O3 has been prepared and its optical and thermodynamical properties were studied as a function of the ion size of the lanthanide element and its atomic masses.
34389396	6	38	theme	ion	903:905	arg1	size					907:910	ion size	903:910	ion size	903:910	The variation of all the said properties with ion size is opposite to that of atomic mass due to lanthanide contraction.
34389396	4	39	theme	relaxation	619:628	arg1	strength					630:637	relaxation strength	619:637	relaxation strength	619:637	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
34389396	4	40	theme	waves	553:557	arg1	propagation					527:537	The propagation	523:537	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β)	523:729	The propagation of ultrasonic waves like ultrasonic velocity and its derived quantities such as relaxation strength (rs), adiabatic bulk modulus (Ks), acoustic impedance (Z) and adiabatic compressibility (β) have been obtained for different Chitosan-Lanthanide oxides (Ch-LnO).
32938382	5	0	theme	binding	995:1001	arg1	affinity					1003:1010	its binding affinity	991:1010	its binding affinity to EPS	991:1017	Although excessive levels of calcium did not impair the growth parameters, it also did not have a positive effect on biofilm formation or its binding affinity to EPS.
32938382	9	1	theme	surrounding	1799:1809	arg1	environment					1811:1821	the surrounding environment	1795:1821	the surrounding environment	1795:1821	Additionally, Zn2+ had almost always the lowest affinity to all EPS; thus, the unbound zinc could also still remain in the surrounding environment and keep its antimicrobial properties.
32938382	10	2	theme	stable	1903:1908	arg1	relationship					1910:1921	a stable relationship	1901:1921	a stable relationship between calcium, magnesium and zinc	1901:1957	CONCLUSION It is important to maintain a stable relationship between calcium, magnesium and zinc as excessive concentrations of one can easily destroy the balance between the three in cariogenic environment and lead to progression of the disease.
32938382	7	3	theme	strengthening	1405:1417	arg1	structure					1423:1431	the biofilm strengthening its structure	1393:1431	the biofilm strengthening its structure	1393:1431	Thus, this supports the idea of self-regulation, when more Ca2+ is released, more calcium is bound to the biofilm strengthening its structure and however, also less is left for remineralization.
32938382	3	4	theme	mutans	580:585	arg1	strains					587:593	S. mutans strains	577:593	RESULTS S. mutans strains used in this study	569:612	RESULTS S. mutans strains used in this study show the highest tolerance towards calcium of the ions tested.
32938382	2	5	theme	bacterial	554:562	arg1	EPS					564:566	the bacterial EPS	550:566	the bacterial EPS	550:566	At the moment, little is known about the adsorption of metals by cariogenic EPS; thus, the aim of the present study was to evaluate the effect of divalent ions (calcium, magnesium, and zinc) on the growth and biofilm formation of mutans streptococci and on the dissolution of hydroxyapatite as well as to investigate their binding to the bacterial EPS.
32938382	9	6	theme	lowest	1717:1722	arg1	affinity					1724:1731	the lowest affinity	1713:1731	the lowest affinity to all EPS	1713:1742	Additionally, Zn2+ had almost always the lowest affinity to all EPS; thus, the unbound zinc could also still remain in the surrounding environment and keep its antimicrobial properties.
32938382	2	7	theme	biofilm	425:431	arg1	formation					433:441	biofilm formation	425:441	biofilm formation	425:441	At the moment, little is known about the adsorption of metals by cariogenic EPS; thus, the aim of the present study was to evaluate the effect of divalent ions (calcium, magnesium, and zinc) on the growth and biofilm formation of mutans streptococci and on the dissolution of hydroxyapatite as well as to investigate their binding to the bacterial EPS.
32938382	0	8	from	Effect	0:5	arg1	formation					46:54	cariogenic biofilm formation	27:54	cariogenic biofilm formation	27:54	Effect of divalent ions on cariogenic biofilm formation.
32938382	2	9	theme	hydroxyapatite	492:505	arg1	dissolution					477:487	the dissolution	473:487	the dissolution of hydroxyapatite	473:505	At the moment, little is known about the adsorption of metals by cariogenic EPS; thus, the aim of the present study was to evaluate the effect of divalent ions (calcium, magnesium, and zinc) on the growth and biofilm formation of mutans streptococci and on the dissolution of hydroxyapatite as well as to investigate their binding to the bacterial EPS.
32938382	3	10	dep	RESULTS	569:575	arg1	strains					587:593	S. mutans strains	577:593	RESULTS S. mutans strains used in this study	569:612	RESULTS S. mutans strains used in this study show the highest tolerance towards calcium of the ions tested.
32938382	5	11	theme	excessive	862:870	arg1	levels					872:877	excessive levels	862:877	excessive levels of calcium	862:888	Although excessive levels of calcium did not impair the growth parameters, it also did not have a positive effect on biofilm formation or its binding affinity to EPS.
32938382	1	12	theme	BACKGROUND	57:66	arg1	cations					77:83	BACKGROUND Divalent cations	57:83	BACKGROUND Divalent cations	57:83	BACKGROUND Divalent cations are able to interact with exopolysaccharides (EPS) and thus are capable to modify the structure and composition of dental biofilm.
32938382	7	13	theme	biofilm	1397:1403	arg1	structure					1423:1431	the biofilm strengthening its structure	1393:1431	the biofilm strengthening its structure	1393:1431	Thus, this supports the idea of self-regulation, when more Ca2+ is released, more calcium is bound to the biofilm strengthening its structure and however, also less is left for remineralization.
32938382	5	14	theme	biofilm	970:976	arg1	formation					978:986	biofilm formation	970:986	biofilm formation	970:986	Although excessive levels of calcium did not impair the growth parameters, it also did not have a positive effect on biofilm formation or its binding affinity to EPS.
32938382	1	15	theme	Divalent	68:75	arg1	cations					77:83	BACKGROUND Divalent cations	57:83	BACKGROUND Divalent cations	57:83	BACKGROUND Divalent cations are able to interact with exopolysaccharides (EPS) and thus are capable to modify the structure and composition of dental biofilm.
32938382	8	16	contain	had	1554:1556	arg2	effect					1581:1586	a strong antibacterial effect	1558:1586	a strong antibacterial effect on the strains as well as on calcium dissolution	1558:1635	Zinc inhibited bacterial adhesion already at low concentrations and had a strong antibacterial effect on the strains as well as on calcium dissolution; leading to less biofilm and less EPS.
32938382	8	16	contain	had	1554:1556	arg1	Zinc					1486:1489	Zinc	1486:1489	Zinc	1486:1489	Zinc inhibited bacterial adhesion already at low concentrations and had a strong antibacterial effect on the strains as well as on calcium dissolution; leading to less biofilm and less EPS.
32938382	6	17	theme	tooth	1135:1139	arg1	surface					1141:1147	the tooth surface	1131:1147	the tooth surface	1131:1147	Magnesium-saturated environment proved to be counterproductive as strains were able to dissolve more Ca2+ from the tooth surface in the presence of magnesium, therefore releasing excessive amounts of Ca2+ in the environment and leading to the progression of the disease.
32938382	9	18	theme	antimicrobial	1836:1848	arg1	properties					1850:1859	its antimicrobial properties	1832:1859	its antimicrobial properties	1832:1859	Additionally, Zn2+ had almost always the lowest affinity to all EPS; thus, the unbound zinc could also still remain in the surrounding environment and keep its antimicrobial properties.
32938382	8	19	theme	antibacterial	1567:1579	arg1	effect					1581:1586	a strong antibacterial effect	1558:1586	a strong antibacterial effect on the strains as well as on calcium dissolution	1558:1635	Zinc inhibited bacterial adhesion already at low concentrations and had a strong antibacterial effect on the strains as well as on calcium dissolution; leading to less biofilm and less EPS.
32938382	2	20	theme	study	326:330	arg1	aim					307:309	the aim	303:309	the aim of the present study	303:330	At the moment, little is known about the adsorption of metals by cariogenic EPS; thus, the aim of the present study was to evaluate the effect of divalent ions (calcium, magnesium, and zinc) on the growth and biofilm formation of mutans streptococci and on the dissolution of hydroxyapatite as well as to investigate their binding to the bacterial EPS.
32938382	7	21	theme	self-regulation	1323:1337	arg1	idea					1315:1318	the idea	1311:1318	the idea of self-regulation	1311:1337	Thus, this supports the idea of self-regulation, when more Ca2+ is released, more calcium is bound to the biofilm strengthening its structure and however, also less is left for remineralization.
32938382	2	22	theme	present	318:324	arg1	study					326:330	the present study	314:330	the present study	314:330	At the moment, little is known about the adsorption of metals by cariogenic EPS; thus, the aim of the present study was to evaluate the effect of divalent ions (calcium, magnesium, and zinc) on the growth and biofilm formation of mutans streptococci and on the dissolution of hydroxyapatite as well as to investigate their binding to the bacterial EPS.
32938382	2	23	theme	ions	371:374	arg1	effect					352:357	the effect	348:357	the effect of divalent ions (calcium, magnesium, and zinc) on the growth and biofilm formation of mutans streptococci and on the dissolution of hydroxyapatite	348:505	At the moment, little is known about the adsorption of metals by cariogenic EPS; thus, the aim of the present study was to evaluate the effect of divalent ions (calcium, magnesium, and zinc) on the growth and biofilm formation of mutans streptococci and on the dissolution of hydroxyapatite as well as to investigate their binding to the bacterial EPS.
32938382	2	24	dep	ions	371:374	arg1	ions					371:374	divalent ions	362:374	divalent ions (calcium, magnesium, and zinc)	362:405	At the moment, little is known about the adsorption of metals by cariogenic EPS; thus, the aim of the present study was to evaluate the effect of divalent ions (calcium, magnesium, and zinc) on the growth and biofilm formation of mutans streptococci and on the dissolution of hydroxyapatite as well as to investigate their binding to the bacterial EPS.
32938382	2	24	dep	ions	371:374	arg1	magnesium					386:394	magnesium	386:394	magnesium	386:394	At the moment, little is known about the adsorption of metals by cariogenic EPS; thus, the aim of the present study was to evaluate the effect of divalent ions (calcium, magnesium, and zinc) on the growth and biofilm formation of mutans streptococci and on the dissolution of hydroxyapatite as well as to investigate their binding to the bacterial EPS.
32938382	2	24	dep	ions	371:374	arg1	zinc					401:404	zinc	401:404	zinc	401:404	At the moment, little is known about the adsorption of metals by cariogenic EPS; thus, the aim of the present study was to evaluate the effect of divalent ions (calcium, magnesium, and zinc) on the growth and biofilm formation of mutans streptococci and on the dissolution of hydroxyapatite as well as to investigate their binding to the bacterial EPS.
32938382	2	24	dep	ions	371:374	arg1	calcium					377:383	calcium	377:383	calcium	377:383	At the moment, little is known about the adsorption of metals by cariogenic EPS; thus, the aim of the present study was to evaluate the effect of divalent ions (calcium, magnesium, and zinc) on the growth and biofilm formation of mutans streptococci and on the dissolution of hydroxyapatite as well as to investigate their binding to the bacterial EPS.
32938382	2	25	theme	metals	271:276	arg1	adsorption					257:266	the adsorption	253:266	the adsorption of metals by cariogenic EPS	253:294	At the moment, little is known about the adsorption of metals by cariogenic EPS; thus, the aim of the present study was to evaluate the effect of divalent ions (calcium, magnesium, and zinc) on the growth and biofilm formation of mutans streptococci and on the dissolution of hydroxyapatite as well as to investigate their binding to the bacterial EPS.
32938382	10	26	theme	excessive	1962:1970	arg1	concentrations					1972:1985	excessive concentrations	1962:1985	excessive concentrations of one	1962:1992	CONCLUSION It is important to maintain a stable relationship between calcium, magnesium and zinc as excessive concentrations of one can easily destroy the balance between the three in cariogenic environment and lead to progression of the disease.
32938382	2	27	theme	divalent	362:369	arg1	ions					371:374	divalent ions	362:374	divalent ions (calcium, magnesium, and zinc)	362:405	At the moment, little is known about the adsorption of metals by cariogenic EPS; thus, the aim of the present study was to evaluate the effect of divalent ions (calcium, magnesium, and zinc) on the growth and biofilm formation of mutans streptococci and on the dissolution of hydroxyapatite as well as to investigate their binding to the bacterial EPS.
32938382	2	27	theme	divalent	362:369	arg1	magnesium					386:394	magnesium	386:394	magnesium	386:394	At the moment, little is known about the adsorption of metals by cariogenic EPS; thus, the aim of the present study was to evaluate the effect of divalent ions (calcium, magnesium, and zinc) on the growth and biofilm formation of mutans streptococci and on the dissolution of hydroxyapatite as well as to investigate their binding to the bacterial EPS.
32938382	2	27	theme	divalent	362:369	arg1	zinc					401:404	zinc	401:404	zinc	401:404	At the moment, little is known about the adsorption of metals by cariogenic EPS; thus, the aim of the present study was to evaluate the effect of divalent ions (calcium, magnesium, and zinc) on the growth and biofilm formation of mutans streptococci and on the dissolution of hydroxyapatite as well as to investigate their binding to the bacterial EPS.
32938382	2	27	theme	divalent	362:369	arg1	calcium					377:383	calcium	377:383	calcium	377:383	At the moment, little is known about the adsorption of metals by cariogenic EPS; thus, the aim of the present study was to evaluate the effect of divalent ions (calcium, magnesium, and zinc) on the growth and biofilm formation of mutans streptococci and on the dissolution of hydroxyapatite as well as to investigate their binding to the bacterial EPS.
32938382	2	28	theme	streptococci	453:464	arg1	growth					414:419	the growth	410:419	the growth	410:419	At the moment, little is known about the adsorption of metals by cariogenic EPS; thus, the aim of the present study was to evaluate the effect of divalent ions (calcium, magnesium, and zinc) on the growth and biofilm formation of mutans streptococci and on the dissolution of hydroxyapatite as well as to investigate their binding to the bacterial EPS.
32938382	10	29	theme	cariogenic	2046:2055	arg1	environment					2057:2067	cariogenic environment	2046:2067	cariogenic environment	2046:2067	CONCLUSION It is important to maintain a stable relationship between calcium, magnesium and zinc as excessive concentrations of one can easily destroy the balance between the three in cariogenic environment and lead to progression of the disease.
32938382	0	30	theme	divalent	10:17	arg1	ions					19:22	divalent ions	10:22	divalent ions	10:22	Effect of divalent ions on cariogenic biofilm formation.
32938382	3	31	theme	highest	623:629	arg1	tolerance					631:639	the highest tolerance	619:639	the highest tolerance towards calcium of the ions tested	619:674	RESULTS S. mutans strains used in this study show the highest tolerance towards calcium of the ions tested.
32938382	4	32	theme	higher	800:805	arg1	concentration					807:819	higher concentration	800:819	higher concentration of calcium in the surroundings	800:850	Growth parameters showed no differences to control condition for both strains up to 100 mM; revealing natural tolerance to higher concentration of calcium in the surroundings.
32938382	8	33	theme	strong	1560:1565	arg1	effect					1581:1586	a strong antibacterial effect	1558:1586	a strong antibacterial effect on the strains as well as on calcium dissolution	1558:1635	Zinc inhibited bacterial adhesion already at low concentrations and had a strong antibacterial effect on the strains as well as on calcium dissolution; leading to less biofilm and less EPS.
32938382	6	34	theme	Magnesium-saturated	1020:1038	arg1	environment					1040:1050	Magnesium-saturated environment	1020:1050	Magnesium-saturated environment	1020:1050	Magnesium-saturated environment proved to be counterproductive as strains were able to dissolve more Ca2+ from the tooth surface in the presence of magnesium, therefore releasing excessive amounts of Ca2+ in the environment and leading to the progression of the disease.
32938382	0	35	theme	cariogenic	27:36	arg1	formation					46:54	cariogenic biofilm formation	27:54	cariogenic biofilm formation	27:54	Effect of divalent ions on cariogenic biofilm formation.
32938382	4	36	theme	calcium	824:830	arg1	concentration					807:819	higher concentration	800:819	higher concentration of calcium in the surroundings	800:850	Growth parameters showed no differences to control condition for both strains up to 100 mM; revealing natural tolerance to higher concentration of calcium in the surroundings.
32938382	9	37	contain	had	1695:1697	arg2	affinity					1724:1731	the lowest affinity	1713:1731	the lowest affinity to all EPS	1713:1742	Additionally, Zn2+ had almost always the lowest affinity to all EPS; thus, the unbound zinc could also still remain in the surrounding environment and keep its antimicrobial properties.
32938382	9	37	contain	had	1695:1697	arg1	Zn2+					1690:1693	Zn2+	1690:1693	Zn2+	1690:1693	Additionally, Zn2+ had almost always the lowest affinity to all EPS; thus, the unbound zinc could also still remain in the surrounding environment and keep its antimicrobial properties.
32938382	9	38	theme	unbound	1755:1761	arg1	zinc					1763:1766	the unbound zinc	1751:1766	the unbound zinc	1751:1766	Additionally, Zn2+ had almost always the lowest affinity to all EPS; thus, the unbound zinc could also still remain in the surrounding environment and keep its antimicrobial properties.
32938382	10	39	from	balance	2017:2023	arg1	environment					2057:2067	cariogenic environment	2046:2067	cariogenic environment	2046:2067	CONCLUSION It is important to maintain a stable relationship between calcium, magnesium and zinc as excessive concentrations of one can easily destroy the balance between the three in cariogenic environment and lead to progression of the disease.
32938382	6	40	theme	disease	1282:1288	arg1	progression					1263:1273	the progression	1259:1273	the progression of the disease	1259:1288	Magnesium-saturated environment proved to be counterproductive as strains were able to dissolve more Ca2+ from the tooth surface in the presence of magnesium, therefore releasing excessive amounts of Ca2+ in the environment and leading to the progression of the disease.
32938382	3	41	theme	ions	664:667	arg1	tolerance					631:639	the highest tolerance	619:639	the highest tolerance towards calcium of the ions tested	619:674	RESULTS S. mutans strains used in this study show the highest tolerance towards calcium of the ions tested.
32938382	6	42	theme	excessive	1199:1207	arg1	amounts					1209:1215	excessive amounts	1199:1215	excessive amounts of Ca2+	1199:1223	Magnesium-saturated environment proved to be counterproductive as strains were able to dissolve more Ca2+ from the tooth surface in the presence of magnesium, therefore releasing excessive amounts of Ca2+ in the environment and leading to the progression of the disease.
32938382	6	42	theme	excessive	1199:1207	arg1	Ca2+					1220:1223	Ca2+	1220:1223	Ca2+	1220:1223	Magnesium-saturated environment proved to be counterproductive as strains were able to dissolve more Ca2+ from the tooth surface in the presence of magnesium, therefore releasing excessive amounts of Ca2+ in the environment and leading to the progression of the disease.
32938382	4	43	from	concentration	807:819	arg1	surroundings					839:850	the surroundings	835:850	the surroundings	835:850	Growth parameters showed no differences to control condition for both strains up to 100 mM; revealing natural tolerance to higher concentration of calcium in the surroundings.
32938382	0	44	theme	ions	19:22	arg1	Effect					0:5	Effect	0:5	Effect of divalent ions on cariogenic biofilm formation.	0:55	Effect of divalent ions on cariogenic biofilm formation.
32938382	4	45	theme	natural	779:785	arg1	tolerance					787:795	natural tolerance	779:795	natural tolerance to higher concentration of calcium in the surroundings	779:850	Growth parameters showed no differences to control condition for both strains up to 100 mM; revealing natural tolerance to higher concentration of calcium in the surroundings.
32938382	4	46	theme	control	720:726	arg1	condition					728:736	control condition	720:736	control condition	720:736	Growth parameters showed no differences to control condition for both strains up to 100 mM; revealing natural tolerance to higher concentration of calcium in the surroundings.
32938382	10	47	theme	one	1990:1992	arg1	concentrations					1972:1985	excessive concentrations	1962:1985	excessive concentrations of one	1962:1992	CONCLUSION It is important to maintain a stable relationship between calcium, magnesium and zinc as excessive concentrations of one can easily destroy the balance between the three in cariogenic environment and lead to progression of the disease.
32938382	0	48	theme	biofilm	38:44	arg1	formation					46:54	cariogenic biofilm formation	27:54	cariogenic biofilm formation	27:54	Effect of divalent ions on cariogenic biofilm formation.
32938382	6	49	theme	Ca2+	1220:1223	arg1	amounts					1209:1215	excessive amounts	1199:1215	excessive amounts of Ca2+	1199:1223	Magnesium-saturated environment proved to be counterproductive as strains were able to dissolve more Ca2+ from the tooth surface in the presence of magnesium, therefore releasing excessive amounts of Ca2+ in the environment and leading to the progression of the disease.
32938382	6	49	theme	Ca2+	1220:1223	arg1	Ca2+					1220:1223	Ca2+	1220:1223	Ca2+	1220:1223	Magnesium-saturated environment proved to be counterproductive as strains were able to dissolve more Ca2+ from the tooth surface in the presence of magnesium, therefore releasing excessive amounts of Ca2+ in the environment and leading to the progression of the disease.
32938382	5	50	theme	positive	951:958	arg1	effect					960:965	a positive effect	949:965	a positive effect	949:965	Although excessive levels of calcium did not impair the growth parameters, it also did not have a positive effect on biofilm formation or its binding affinity to EPS.
32938382	8	51	theme	bacterial	1501:1509	arg1	adhesion					1511:1518	bacterial adhesion	1501:1518	bacterial adhesion	1501:1518	Zinc inhibited bacterial adhesion already at low concentrations and had a strong antibacterial effect on the strains as well as on calcium dissolution; leading to less biofilm and less EPS.
32938382	8	52	from	effect	1581:1586	arg1	dissolution					1625:1635	calcium dissolution	1617:1635	calcium dissolution	1617:1635	Zinc inhibited bacterial adhesion already at low concentrations and had a strong antibacterial effect on the strains as well as on calcium dissolution; leading to less biofilm and less EPS.
32938382	8	52	from	effect	1581:1586	arg1	strains					1595:1601	the strains	1591:1601	the strains	1591:1601	Zinc inhibited bacterial adhesion already at low concentrations and had a strong antibacterial effect on the strains as well as on calcium dissolution; leading to less biofilm and less EPS.
32938382	3	53	theme	S.	577:578	arg1	strains					587:593	S. mutans strains	577:593	RESULTS S. mutans strains used in this study	569:612	RESULTS S. mutans strains used in this study show the highest tolerance towards calcium of the ions tested.
32938382	4	54	theme	Growth	677:682	arg1	parameters					684:693	Growth parameters	677:693	Growth parameters	677:693	Growth parameters showed no differences to control condition for both strains up to 100 mM; revealing natural tolerance to higher concentration of calcium in the surroundings.
32938382	5	55	theme	growth	909:914	arg1	parameters					916:925	the growth parameters	905:925	the growth parameters	905:925	Although excessive levels of calcium did not impair the growth parameters, it also did not have a positive effect on biofilm formation or its binding affinity to EPS.
32938382	10	56	theme	disease	2100:2106	arg1	progression					2081:2091	progression	2081:2091	progression of the disease	2081:2106	CONCLUSION It is important to maintain a stable relationship between calcium, magnesium and zinc as excessive concentrations of one can easily destroy the balance between the three in cariogenic environment and lead to progression of the disease.
32938382	1	57	theme	dental	200:205	arg1	biofilm					207:213	dental biofilm	200:213	dental biofilm	200:213	BACKGROUND Divalent cations are able to interact with exopolysaccharides (EPS) and thus are capable to modify the structure and composition of dental biofilm.
32938382	6	58	theme	magnesium	1168:1176	arg1	presence					1156:1163	the presence	1152:1163	the presence of magnesium	1152:1176	Magnesium-saturated environment proved to be counterproductive as strains were able to dissolve more Ca2+ from the tooth surface in the presence of magnesium, therefore releasing excessive amounts of Ca2+ in the environment and leading to the progression of the disease.
32938382	2	59	theme	cariogenic	281:290	arg1	EPS					292:294	cariogenic EPS	281:294	cariogenic EPS	281:294	At the moment, little is known about the adsorption of metals by cariogenic EPS; thus, the aim of the present study was to evaluate the effect of divalent ions (calcium, magnesium, and zinc) on the growth and biofilm formation of mutans streptococci and on the dissolution of hydroxyapatite as well as to investigate their binding to the bacterial EPS.
32938382	1	60	theme	biofilm	207:213	arg1	composition					185:195	composition	185:195	composition	185:195	BACKGROUND Divalent cations are able to interact with exopolysaccharides (EPS) and thus are capable to modify the structure and composition of dental biofilm.
32938382	1	60	theme	biofilm	207:213	arg1	structure					171:179	structure	171:179	structure	171:179	BACKGROUND Divalent cations are able to interact with exopolysaccharides (EPS) and thus are capable to modify the structure and composition of dental biofilm.
32938382	1	61	dep	structure	171:179	arg1	the					167:169	the	167:169	the	167:169	BACKGROUND Divalent cations are able to interact with exopolysaccharides (EPS) and thus are capable to modify the structure and composition of dental biofilm.
32938382	8	62	theme	calcium	1617:1623	arg1	dissolution					1625:1635	calcium dissolution	1617:1635	calcium dissolution	1617:1635	Zinc inhibited bacterial adhesion already at low concentrations and had a strong antibacterial effect on the strains as well as on calcium dissolution; leading to less biofilm and less EPS.
32938382	10	63	dep	CONCLUSION	1862:1871	arg1	important					1879:1887	important	1879:1887	important	1879:1887	CONCLUSION It is important to maintain a stable relationship between calcium, magnesium and zinc as excessive concentrations of one can easily destroy the balance between the three in cariogenic environment and lead to progression of the disease.
32938382	5	64	theme	calcium	882:888	arg1	levels					872:877	excessive levels	862:877	excessive levels of calcium	862:888	Although excessive levels of calcium did not impair the growth parameters, it also did not have a positive effect on biofilm formation or its binding affinity to EPS.
32938382	5	65	contain	have	944:947	arg2	effect					960:965	a positive effect	949:965	a positive effect	949:965	Although excessive levels of calcium did not impair the growth parameters, it also did not have a positive effect on biofilm formation or its binding affinity to EPS.
32938382	5	65	contain	have	944:947	arg1	it					928:929	it	928:929	it	928:929	Although excessive levels of calcium did not impair the growth parameters, it also did not have a positive effect on biofilm formation or its binding affinity to EPS.
32938382	2	66	from	effect	352:357	arg1	growth					414:419	the growth	410:419	the growth	410:419	At the moment, little is known about the adsorption of metals by cariogenic EPS; thus, the aim of the present study was to evaluate the effect of divalent ions (calcium, magnesium, and zinc) on the growth and biofilm formation of mutans streptococci and on the dissolution of hydroxyapatite as well as to investigate their binding to the bacterial EPS.
32938382	2	66	from	effect	352:357	arg1	dissolution					477:487	the dissolution	473:487	the dissolution of hydroxyapatite	473:505	At the moment, little is known about the adsorption of metals by cariogenic EPS; thus, the aim of the present study was to evaluate the effect of divalent ions (calcium, magnesium, and zinc) on the growth and biofilm formation of mutans streptococci and on the dissolution of hydroxyapatite as well as to investigate their binding to the bacterial EPS.
32938382	8	67	theme	low	1531:1533	arg1	concentrations					1535:1548	low concentrations	1531:1548	low concentrations	1531:1548	Zinc inhibited bacterial adhesion already at low concentrations and had a strong antibacterial effect on the strains as well as on calcium dissolution; leading to less biofilm and less EPS.
33664475	10	0	from	alterations	1521:1531	arg1	electrophysiology					1583:1599	hippocampal electrophysiology	1571:1599	hippocampal electrophysiology	1571:1599	Compared to control mice, WT offspring deprived of 6'SL during lactation exhibited consistent alterations in all cognitive functions addressed, hippocampal electrophysiology, and in pathways regulating the serotonergic system (identified through gut microbiota and plasma metabolomics).
33664475	10	0	from	alterations	1521:1531	arg1	pathways					1609:1616	pathways	1609:1616	pathways regulating the serotonergic system (identified through gut microbiota and plasma metabolomics)	1609:1711	Compared to control mice, WT offspring deprived of 6'SL during lactation exhibited consistent alterations in all cognitive functions addressed, hippocampal electrophysiology, and in pathways regulating the serotonergic system (identified through gut microbiota and plasma metabolomics).
33664475	10	0	from	alterations	1521:1531	arg1	functions					1550:1558	all cognitive functions	1536:1558	all cognitive functions addressed	1536:1568	Compared to control mice, WT offspring deprived of 6'SL during lactation exhibited consistent alterations in all cognitive functions addressed, hippocampal electrophysiology, and in pathways regulating the serotonergic system (identified through gut microbiota and plasma metabolomics).
33664475	4	1	theme	sialyl	610:615	arg1	lactose					626:632	sialyl(alpha2,6)lactose	610:632	sialyl(alpha2,6)lactose (6'SL)	610:639	We capitalized on a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring.
33664475	4	1	theme	sialyl	610:615	arg1	sources					657:663	the two sources	649:663	the two sources of sialic acid (Neu5Ac) to the lactating offspring	649:714	We capitalized on a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring.
33664475	4	1	theme	sialyl	610:615	arg1	SL					637:638	6'SL	635:638	6'SL	635:638	We capitalized on a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring.
33664475	4	1	theme	sialyl	610:615	arg1	one					642:644	one	642:644	one	642:644	We capitalized on a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring.
33664475	10	2	theme	gut	1673:1675	arg1	microbiota					1677:1686	gut microbiota	1673:1686	gut microbiota	1673:1686	Compared to control mice, WT offspring deprived of 6'SL during lactation exhibited consistent alterations in all cognitive functions addressed, hippocampal electrophysiology, and in pathways regulating the serotonergic system (identified through gut microbiota and plasma metabolomics).
33664475	6	3	theme	wild-type	861:869	arg1	pups					876:879	cross-fostered newborn wild-type (WT) pups	838:879	cross-fostered newborn wild-type (WT) pups	838:879	To deprive lactating offspring of 6'SL, we cross-fostered newborn wild-type (WT) pups to KO dams, which provide 6'SL-deficient milk.
33664475	4	4	theme	alpha2,6	617:624	arg1	lactose					626:632	sialyl(alpha2,6)lactose	610:632	sialyl(alpha2,6)lactose (6'SL)	610:639	We capitalized on a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring.
33664475	4	4	theme	alpha2,6	617:624	arg1	sources					657:663	the two sources	649:663	the two sources of sialic acid (Neu5Ac) to the lactating offspring	649:714	We capitalized on a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring.
33664475	4	4	theme	alpha2,6	617:624	arg1	SL					637:638	6'SL	635:638	6'SL	635:638	We capitalized on a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring.
33664475	4	4	theme	alpha2,6	617:624	arg1	one					642:644	one	642:644	one	642:644	We capitalized on a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring.
33664475	12	5	theme	short-term	1947:1956	arg1	upregulation					1958:1969	a short-term upregulation	1945:1969	a short-term upregulation of genes modulating neuronal patterning in the PFC	1945:2020	Our data suggest that 6'SL in maternal milk adjusts cognitive development through a short-term upregulation of genes modulating neuronal patterning in the PFC.
33664475	6	6	theme	cross-fostered	838:851	arg1	pups					876:879	cross-fostered newborn wild-type (WT) pups	838:879	cross-fostered newborn wild-type (WT) pups	838:879	To deprive lactating offspring of 6'SL, we cross-fostered newborn wild-type (WT) pups to KO dams, which provide 6'SL-deficient milk.
33664475	3	7	theme	HMO	475:477	arg1	deprivation					438:448	a selective lactational deprivation	414:448	a selective lactational deprivation of a specific sialylated HMO in mice	414:485	Here, we investigated the long-term consequences of a selective lactational deprivation of a specific sialylated HMO in mice.
33664475	8	8	theme	microbiota	1185:1194	arg1	composition					1196:1206	gut microbiota composition	1181:1206	gut microbiota composition	1181:1206	To detail the associated endophenotypes, we investigated hippocampal electrophysiology, plasma metabolomics, and gut microbiota composition.
33664475	2	9	theme	selective	303:311	arg1	role					313:316	their selective role	297:316	their selective role	297:316	While sialylated human milk oligosaccharides (HMOs) have been implicated in phenotypic programming, their selective role and underlying mechanisms remained elusive.
33664475	8	10	theme	hippocampal	1125:1135	arg1	electrophysiology					1137:1153	hippocampal electrophysiology	1125:1153	hippocampal electrophysiology	1125:1153	To detail the associated endophenotypes, we investigated hippocampal electrophysiology, plasma metabolomics, and gut microbiota composition.
33664475	12	11	theme	cognitive	1915:1923	arg1	development					1925:1935	cognitive development	1915:1935	cognitive development	1915:1935	Our data suggest that 6'SL in maternal milk adjusts cognitive development through a short-term upregulation of genes modulating neuronal patterning in the PFC.
33664475	11	12	theme	site-	1743:1747	arg1	expression					1795:1804	a site- (PFC) and time-specific (eye-opening) reduced expression	1741:1804	a site- (PFC) and time-specific (eye-opening) reduced expression of genes involved in central nervous system development	1741:1860	These were associated with a site- (PFC) and time-specific (eye-opening) reduced expression of genes involved in central nervous system development.
33664475	11	13	theme	central	1827:1833	arg1	system					1843:1848	central nervous system	1827:1848	central nervous system development	1827:1860	These were associated with a site- (PFC) and time-specific (eye-opening) reduced expression of genes involved in central nervous system development.
33664475	6	14	theme	KO	884:885	arg1	dams					887:890	KO dams	884:890	KO dams	884:890	To deprive lactating offspring of 6'SL, we cross-fostered newborn wild-type (WT) pups to KO dams, which provide 6'SL-deficient milk.
33664475	7	15	theme	cognitive	981:989	arg1	capabilities					991:1002	cognitive capabilities	981:1002	cognitive capabilities	981:1002	To test whether lactational 6'SL deprivation affects cognitive capabilities in adulthood, we assessed attention, perseveration, and memory.
33664475	1	16	theme	essential	150:158	arg1	molecules					140:148	bioactive molecules	130:148	bioactive molecules essential for brain and cognitive development	130:194	Breastmilk contains bioactive molecules essential for brain and cognitive development.
33664475	11	17	dep	site-	1743:1747	arg1	PFC					1750:1752	PFC	1750:1752	PFC	1750:1752	These were associated with a site- (PFC) and time-specific (eye-opening) reduced expression of genes involved in central nervous system development.
33664475	2	18	theme	phenotypic	273:282	arg1	programming					284:294	phenotypic programming	273:294	phenotypic programming	273:294	While sialylated human milk oligosaccharides (HMOs) have been implicated in phenotypic programming, their selective role and underlying mechanisms remained elusive.
33664475	1	19	theme	cognitive	174:182	arg1	development					184:194	cognitive development	174:194	cognitive development	174:194	Breastmilk contains bioactive molecules essential for brain and cognitive development.
33664475	4	20	theme	mouse	523:527	arg1	model					529:533	a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J)	506:558	a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring	506:714	We capitalized on a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring.
33664475	4	21	theme	acid	675:678	arg1	sources					657:663	the two sources	649:663	the two sources of sialic acid (Neu5Ac) to the lactating offspring	649:714	We capitalized on a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring.
33664475	0	22	theme	transmission	91:102	arg1	mode					104:107	a non-genomic transmission mode	77:107	a non-genomic transmission mode	77:107	Sialylated human milk oligosaccharides program cognitive development through a non-genomic transmission mode.
33664475	2	23	gly	sialylated	203:212	arg1	HMOs					243:246	HMOs	243:246	HMOs	243:246	While sialylated human milk oligosaccharides (HMOs) have been implicated in phenotypic programming, their selective role and underlying mechanisms remained elusive.
33664475	2	23	gly	sialylated	203:212	arg1	oligosaccharides					225:240	sialylated human milk oligosaccharides	203:240	sialylated human milk oligosaccharides (HMOs)	203:247	While sialylated human milk oligosaccharides (HMOs) have been implicated in phenotypic programming, their selective role and underlying mechanisms remained elusive.
33664475	4	24	theme	knock-out	508:516	arg1	model					529:533	a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J)	506:558	a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring	506:714	We capitalized on a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring.
33664475	3	25	theme	lactational	426:436	arg1	deprivation					438:448	a selective lactational deprivation	414:448	a selective lactational deprivation of a specific sialylated HMO in mice	414:485	Here, we investigated the long-term consequences of a selective lactational deprivation of a specific sialylated HMO in mice.
33664475	4	26	theme	KO	519:520	arg1	model					529:533	a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J)	506:558	a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring	506:714	We capitalized on a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring.
33664475	4	27	theme	lactating	696:704	arg1	offspring					706:714	the lactating offspring	692:714	the lactating offspring	692:714	We capitalized on a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring.
33664475	11	28	theme	nervous	1835:1841	arg1	system					1843:1848	central nervous system	1827:1848	central nervous system development	1827:1860	These were associated with a site- (PFC) and time-specific (eye-opening) reduced expression of genes involved in central nervous system development.
33664475	0	29	theme	Sialylated	0:9	arg1	milk					17:20	Sialylated human milk	0:20	Sialylated human milk	0:20	Sialylated human milk oligosaccharides program cognitive development through a non-genomic transmission mode.
33664475	9	30	from	eye-opening	1293:1303	arg1	expression					1278:1287	gene expression	1273:1287	gene expression (at eye-opening and in adulthood) in two brain regions mediating executive functions and memory (hippocampus and prefrontal cortex, PFC)	1273:1424	To investigate the underlying molecular mechanisms, we assessed gene expression (at eye-opening and in adulthood) in two brain regions mediating executive functions and memory (hippocampus and prefrontal cortex, PFC).
33664475	4	31	theme	responsible	577:587	arg1	gene					572:575	the gene	568:575	the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring	568:714	We capitalized on a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring.
33664475	10	32	theme	serotonergic	1633:1644	arg1	system					1646:1651	the serotonergic system	1629:1651	the serotonergic system (identified through gut microbiota and plasma metabolomics)	1629:1711	Compared to control mice, WT offspring deprived of 6'SL during lactation exhibited consistent alterations in all cognitive functions addressed, hippocampal electrophysiology, and in pathways regulating the serotonergic system (identified through gut microbiota and plasma metabolomics).
33664475	6	33	theme	SL	831:832	arg1	offspring					816:824	lactating offspring	806:824	lactating offspring of 6'SL	806:832	To deprive lactating offspring of 6'SL, we cross-fostered newborn wild-type (WT) pups to KO dams, which provide 6'SL-deficient milk.
33664475	1	34	theme	bioactive	130:138	arg1	molecules					140:148	bioactive molecules	130:148	bioactive molecules essential for brain and cognitive development	130:194	Breastmilk contains bioactive molecules essential for brain and cognitive development.
33664475	3	35	theme	specific	455:462	arg1	HMO					475:477	a specific sialylated HMO	453:477	a specific sialylated HMO in mice	453:485	Here, we investigated the long-term consequences of a selective lactational deprivation of a specific sialylated HMO in mice.
33664475	2	36	theme	human	214:218	arg1	HMOs					243:246	HMOs	243:246	HMOs	243:246	While sialylated human milk oligosaccharides (HMOs) have been implicated in phenotypic programming, their selective role and underlying mechanisms remained elusive.
33664475	2	36	theme	human	214:218	arg1	oligosaccharides					225:240	sialylated human milk oligosaccharides	203:240	sialylated human milk oligosaccharides (HMOs)	203:247	While sialylated human milk oligosaccharides (HMOs) have been implicated in phenotypic programming, their selective role and underlying mechanisms remained elusive.
33664475	0	37	theme	program	39:45	arg1	development					57:67	program cognitive development	39:67	program cognitive development	39:67	Sialylated human milk oligosaccharides program cognitive development through a non-genomic transmission mode.
33664475	9	38	dep	hippocampus	1386:1396	arg1	PFC					1421:1423	PFC	1421:1423	PFC	1421:1423	To investigate the underlying molecular mechanisms, we assessed gene expression (at eye-opening and in adulthood) in two brain regions mediating executive functions and memory (hippocampus and prefrontal cortex, PFC).
33664475	5	39	theme	brain	756:760	arg1	structures					762:771	brain structures	756:771	brain structures sustaining cognition	756:792	Neu5Ac is involved in the formation of brain structures sustaining cognition.
33664475	9	40	theme	molecular	1239:1247	arg1	mechanisms					1249:1258	the underlying molecular mechanisms	1224:1258	the underlying molecular mechanisms	1224:1258	To investigate the underlying molecular mechanisms, we assessed gene expression (at eye-opening and in adulthood) in two brain regions mediating executive functions and memory (hippocampus and prefrontal cortex, PFC).
33664475	10	41	theme	WT	1453:1454	arg1	offspring					1456:1464	WT offspring	1453:1464	WT offspring deprived of 6'SL during lactation	1453:1498	Compared to control mice, WT offspring deprived of 6'SL during lactation exhibited consistent alterations in all cognitive functions addressed, hippocampal electrophysiology, and in pathways regulating the serotonergic system (identified through gut microbiota and plasma metabolomics).
33664475	9	42	theme	brain	1330:1334	arg1	regions					1336:1342	two brain regions	1326:1342	two brain regions mediating executive functions and memory (hippocampus and prefrontal cortex, PFC)	1326:1424	To investigate the underlying molecular mechanisms, we assessed gene expression (at eye-opening and in adulthood) in two brain regions mediating executive functions and memory (hippocampus and prefrontal cortex, PFC).
33664475	12	43	theme	neuronal	1991:1998	arg1	patterning					2000:2009	neuronal patterning	1991:2009	neuronal patterning	1991:2009	Our data suggest that 6'SL in maternal milk adjusts cognitive development through a short-term upregulation of genes modulating neuronal patterning in the PFC.
33664475	10	44	theme	plasma	1692:1697	arg1	metabolomics					1699:1710	plasma metabolomics	1692:1710	plasma metabolomics	1692:1710	Compared to control mice, WT offspring deprived of 6'SL during lactation exhibited consistent alterations in all cognitive functions addressed, hippocampal electrophysiology, and in pathways regulating the serotonergic system (identified through gut microbiota and plasma metabolomics).
33664475	9	45	from	expression	1278:1287	arg1	regions					1336:1342	two brain regions	1326:1342	two brain regions mediating executive functions and memory (hippocampus and prefrontal cortex, PFC)	1326:1424	To investigate the underlying molecular mechanisms, we assessed gene expression (at eye-opening and in adulthood) in two brain regions mediating executive functions and memory (hippocampus and prefrontal cortex, PFC).
33664475	9	45	from	expression	1278:1287	arg1	adulthood					1312:1320	adulthood	1312:1320	adulthood	1312:1320	To investigate the underlying molecular mechanisms, we assessed gene expression (at eye-opening and in adulthood) in two brain regions mediating executive functions and memory (hippocampus and prefrontal cortex, PFC).
33664475	11	46	theme	genes	1809:1813	arg1	expression					1795:1804	a site- (PFC) and time-specific (eye-opening) reduced expression	1741:1804	a site- (PFC) and time-specific (eye-opening) reduced expression of genes involved in central nervous system development	1741:1860	These were associated with a site- (PFC) and time-specific (eye-opening) reduced expression of genes involved in central nervous system development.
33664475	12	47	theme	genes	1974:1978	arg1	upregulation					1958:1969	a short-term upregulation	1945:1969	a short-term upregulation of genes modulating neuronal patterning in the PFC	1945:2020	Our data suggest that 6'SL in maternal milk adjusts cognitive development through a short-term upregulation of genes modulating neuronal patterning in the PFC.
33664475	6	48	dep	wild-type	861:869	arg1	WT					872:873	WT	872:873	WT	872:873	To deprive lactating offspring of 6'SL, we cross-fostered newborn wild-type (WT) pups to KO dams, which provide 6'SL-deficient milk.
33664475	10	49	theme	consistent	1510:1519	arg1	alterations					1521:1531	consistent alterations	1510:1531	consistent alterations in all cognitive functions addressed, hippocampal electrophysiology, and in pathways regulating the serotonergic system (identified through gut microbiota and plasma metabolomics)	1510:1711	Compared to control mice, WT offspring deprived of 6'SL during lactation exhibited consistent alterations in all cognitive functions addressed, hippocampal electrophysiology, and in pathways regulating the serotonergic system (identified through gut microbiota and plasma metabolomics).
33664475	2	50	theme	underlying	322:331	arg1	mechanisms					333:342	underlying mechanisms	322:342	underlying mechanisms	322:342	While sialylated human milk oligosaccharides (HMOs) have been implicated in phenotypic programming, their selective role and underlying mechanisms remained elusive.
33664475	8	51	theme	associated	1082:1091	arg1	endophenotypes					1093:1106	the associated endophenotypes	1078:1106	the associated endophenotypes	1078:1106	To detail the associated endophenotypes, we investigated hippocampal electrophysiology, plasma metabolomics, and gut microbiota composition.
33664475	6	52	theme	newborn	853:859	arg1	pups					876:879	cross-fostered newborn wild-type (WT) pups	838:879	cross-fostered newborn wild-type (WT) pups	838:879	To deprive lactating offspring of 6'SL, we cross-fostered newborn wild-type (WT) pups to KO dams, which provide 6'SL-deficient milk.
33664475	8	53	theme	gut	1181:1183	arg1	composition					1196:1206	gut microbiota composition	1181:1206	gut microbiota composition	1181:1206	To detail the associated endophenotypes, we investigated hippocampal electrophysiology, plasma metabolomics, and gut microbiota composition.
33664475	10	54	theme	cognitive	1540:1548	arg1	electrophysiology					1583:1599	hippocampal electrophysiology	1571:1599	hippocampal electrophysiology	1571:1599	Compared to control mice, WT offspring deprived of 6'SL during lactation exhibited consistent alterations in all cognitive functions addressed, hippocampal electrophysiology, and in pathways regulating the serotonergic system (identified through gut microbiota and plasma metabolomics).
33664475	10	54	theme	cognitive	1540:1548	arg1	functions					1550:1558	all cognitive functions	1536:1558	all cognitive functions addressed	1536:1568	Compared to control mice, WT offspring deprived of 6'SL during lactation exhibited consistent alterations in all cognitive functions addressed, hippocampal electrophysiology, and in pathways regulating the serotonergic system (identified through gut microbiota and plasma metabolomics).
33664475	9	55	theme	prefrontal	1402:1411	arg1	cortex					1413:1418	prefrontal cortex	1402:1418	prefrontal cortex	1402:1418	To investigate the underlying molecular mechanisms, we assessed gene expression (at eye-opening and in adulthood) in two brain regions mediating executive functions and memory (hippocampus and prefrontal cortex, PFC).
33664475	9	55	theme	prefrontal	1402:1411	arg1	memory					1378:1383	memory	1378:1383	memory (hippocampus and prefrontal cortex, PFC)	1378:1424	To investigate the underlying molecular mechanisms, we assessed gene expression (at eye-opening and in adulthood) in two brain regions mediating executive functions and memory (hippocampus and prefrontal cortex, PFC).
33664475	12	56	theme	maternal	1893:1900	arg1	milk					1902:1905	maternal milk	1893:1905	maternal milk	1893:1905	Our data suggest that 6'SL in maternal milk adjusts cognitive development through a short-term upregulation of genes modulating neuronal patterning in the PFC.
33664475	6	57	theme	6'SL-deficient	907:920	arg1	milk					922:925	6'SL-deficient milk	907:925	6'SL-deficient milk	907:925	To deprive lactating offspring of 6'SL, we cross-fostered newborn wild-type (WT) pups to KO dams, which provide 6'SL-deficient milk.
33664475	10	58	theme	hippocampal	1571:1581	arg1	electrophysiology					1583:1599	hippocampal electrophysiology	1571:1599	hippocampal electrophysiology	1571:1599	Compared to control mice, WT offspring deprived of 6'SL during lactation exhibited consistent alterations in all cognitive functions addressed, hippocampal electrophysiology, and in pathways regulating the serotonergic system (identified through gut microbiota and plasma metabolomics).
33664475	10	58	theme	hippocampal	1571:1581	arg1	functions					1550:1558	all cognitive functions	1536:1558	all cognitive functions addressed	1536:1568	Compared to control mice, WT offspring deprived of 6'SL during lactation exhibited consistent alterations in all cognitive functions addressed, hippocampal electrophysiology, and in pathways regulating the serotonergic system (identified through gut microbiota and plasma metabolomics).
33664475	8	59	theme	plasma	1156:1161	arg1	metabolomics					1163:1174	plasma metabolomics	1156:1174	plasma metabolomics	1156:1174	To detail the associated endophenotypes, we investigated hippocampal electrophysiology, plasma metabolomics, and gut microbiota composition.
33664475	1	60	contain	contains	121:128	arg1	Breastmilk					110:119	Breastmilk	110:119	Breastmilk	110:119	Breastmilk contains bioactive molecules essential for brain and cognitive development.
33664475	1	60	contain	contains	121:128	arg2	molecules					140:148	bioactive molecules	130:148	bioactive molecules essential for brain and cognitive development	130:194	Breastmilk contains bioactive molecules essential for brain and cognitive development.
33664475	12	61	from	SL	1887:1888	arg1	milk					1902:1905	maternal milk	1893:1905	maternal milk	1893:1905	Our data suggest that 6'SL in maternal milk adjusts cognitive development through a short-term upregulation of genes modulating neuronal patterning in the PFC.
33664475	11	62	theme	time-specific	1759:1771	arg1	expression					1795:1804	a site- (PFC) and time-specific (eye-opening) reduced expression	1741:1804	a site- (PFC) and time-specific (eye-opening) reduced expression of genes involved in central nervous system development	1741:1860	These were associated with a site- (PFC) and time-specific (eye-opening) reduced expression of genes involved in central nervous system development.
33664475	7	63	theme	SL	958:959	arg1	deprivation					961:971	lactational 6'SL deprivation	944:971	lactational 6'SL deprivation	944:971	To test whether lactational 6'SL deprivation affects cognitive capabilities in adulthood, we assessed attention, perseveration, and memory.
33664475	7	64	theme	lactational	944:954	arg1	deprivation					961:971	lactational 6'SL deprivation	944:971	lactational 6'SL deprivation	944:971	To test whether lactational 6'SL deprivation affects cognitive capabilities in adulthood, we assessed attention, perseveration, and memory.
33664475	0	65	theme	non-genomic	79:89	arg1	mode					104:107	a non-genomic transmission mode	77:107	a non-genomic transmission mode	77:107	Sialylated human milk oligosaccharides program cognitive development through a non-genomic transmission mode.
33664475	9	66	theme	underlying	1228:1237	arg1	mechanisms					1249:1258	the underlying molecular mechanisms	1224:1258	the underlying molecular mechanisms	1224:1258	To investigate the underlying molecular mechanisms, we assessed gene expression (at eye-opening and in adulthood) in two brain regions mediating executive functions and memory (hippocampus and prefrontal cortex, PFC).
33664475	4	67	theme	sources	657:663	arg1	lactose					626:632	sialyl(alpha2,6)lactose	610:632	sialyl(alpha2,6)lactose (6'SL)	610:639	We capitalized on a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring.
33664475	4	67	theme	sources	657:663	arg1	sources					657:663	the two sources	649:663	the two sources of sialic acid (Neu5Ac) to the lactating offspring	649:714	We capitalized on a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring.
33664475	4	67	theme	sources	657:663	arg1	one					642:644	one	642:644	one	642:644	We capitalized on a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring.
33664475	3	68	gly	sialylated	464:473	arg1	HMO					475:477	a specific sialylated HMO	453:477	a specific sialylated HMO in mice	453:485	Here, we investigated the long-term consequences of a selective lactational deprivation of a specific sialylated HMO in mice.
33664475	4	69	theme	sialic	668:673	arg1	Neu5Ac					681:686	Neu5Ac	681:686	Neu5Ac	681:686	We capitalized on a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring.
33664475	4	69	theme	sialic	668:673	arg1	acid					675:678	sialic acid	668:678	sialic acid (Neu5Ac) to the lactating offspring	668:714	We capitalized on a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring.
33664475	11	70	dep	time-specific	1759:1771	arg1	eye-opening					1774:1784	eye-opening	1774:1784	eye-opening	1774:1784	These were associated with a site- (PFC) and time-specific (eye-opening) reduced expression of genes involved in central nervous system development.
33664475	3	71	theme	long-term	388:396	arg1	consequences					398:409	the long-term consequences	384:409	the long-term consequences of a selective lactational deprivation of a specific sialylated HMO in mice	384:485	Here, we investigated the long-term consequences of a selective lactational deprivation of a specific sialylated HMO in mice.
33664475	5	72	theme	structures	762:771	arg1	formation					743:751	the formation	739:751	the formation of brain structures sustaining cognition	739:792	Neu5Ac is involved in the formation of brain structures sustaining cognition.
33664475	3	73	from	deprivation	438:448	arg1	mice					482:485	mice	482:485	mice	482:485	Here, we investigated the long-term consequences of a selective lactational deprivation of a specific sialylated HMO in mice.
33664475	9	74	theme	gene	1273:1276	arg1	expression					1278:1287	gene expression	1273:1287	gene expression (at eye-opening and in adulthood) in two brain regions mediating executive functions and memory (hippocampus and prefrontal cortex, PFC)	1273:1424	To investigate the underlying molecular mechanisms, we assessed gene expression (at eye-opening and in adulthood) in two brain regions mediating executive functions and memory (hippocampus and prefrontal cortex, PFC).
33664475	3	75	theme	deprivation	438:448	arg1	consequences					398:409	the long-term consequences	384:409	the long-term consequences of a selective lactational deprivation of a specific sialylated HMO in mice	384:485	Here, we investigated the long-term consequences of a selective lactational deprivation of a specific sialylated HMO in mice.
33664475	10	76	dep	system	1646:1651	arg1	identified					1654:1663	identified	1654:1663	identified through gut microbiota and plasma metabolomics	1654:1710	Compared to control mice, WT offspring deprived of 6'SL during lactation exhibited consistent alterations in all cognitive functions addressed, hippocampal electrophysiology, and in pathways regulating the serotonergic system (identified through gut microbiota and plasma metabolomics).
33664475	11	77	theme	system	1843:1848	arg1	development					1850:1860	central nervous system development	1827:1860	central nervous system development	1827:1860	These were associated with a site- (PFC) and time-specific (eye-opening) reduced expression of genes involved in central nervous system development.
33664475	3	78	theme	selective	416:424	arg1	deprivation					438:448	a selective lactational deprivation	414:448	a selective lactational deprivation of a specific sialylated HMO in mice	414:485	Here, we investigated the long-term consequences of a selective lactational deprivation of a specific sialylated HMO in mice.
33664475	10	79	theme	control	1439:1445	arg1	mice					1447:1450	control mice	1439:1450	control mice	1439:1450	Compared to control mice, WT offspring deprived of 6'SL during lactation exhibited consistent alterations in all cognitive functions addressed, hippocampal electrophysiology, and in pathways regulating the serotonergic system (identified through gut microbiota and plasma metabolomics).
33664475	2	80	theme	milk	220:223	arg1	HMOs					243:246	HMOs	243:246	HMOs	243:246	While sialylated human milk oligosaccharides (HMOs) have been implicated in phenotypic programming, their selective role and underlying mechanisms remained elusive.
33664475	2	80	theme	milk	220:223	arg1	oligosaccharides					225:240	sialylated human milk oligosaccharides	203:240	sialylated human milk oligosaccharides (HMOs)	203:247	While sialylated human milk oligosaccharides (HMOs) have been implicated in phenotypic programming, their selective role and underlying mechanisms remained elusive.
33664475	0	81	theme	cognitive	47:55	arg1	development					57:67	program cognitive development	39:67	program cognitive development	39:67	Sialylated human milk oligosaccharides program cognitive development through a non-genomic transmission mode.
33664475	3	82	theme	sialylated	464:473	arg1	HMO					475:477	a specific sialylated HMO	453:477	a specific sialylated HMO in mice	453:485	Here, we investigated the long-term consequences of a selective lactational deprivation of a specific sialylated HMO in mice.
33664475	2	83	theme	sialylated	203:212	arg1	HMOs					243:246	HMOs	243:246	HMOs	243:246	While sialylated human milk oligosaccharides (HMOs) have been implicated in phenotypic programming, their selective role and underlying mechanisms remained elusive.
33664475	2	83	theme	sialylated	203:212	arg1	oligosaccharides					225:240	sialylated human milk oligosaccharides	203:240	sialylated human milk oligosaccharides (HMOs)	203:247	While sialylated human milk oligosaccharides (HMOs) have been implicated in phenotypic programming, their selective role and underlying mechanisms remained elusive.
33664475	6	84	theme	lactating	806:814	arg1	offspring					816:824	lactating offspring	806:824	lactating offspring of 6'SL	806:832	To deprive lactating offspring of 6'SL, we cross-fostered newborn wild-type (WT) pups to KO dams, which provide 6'SL-deficient milk.
33664475	4	85	theme	lactose	626:632	arg1	synthesis					597:605	the synthesis	593:605	the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring	593:714	We capitalized on a knock-out (KO) mouse model (B6.129-St6gal1tm2Jxm/J) lacking the gene responsible for the synthesis of sialyl(alpha2,6)lactose (6'SL), one of the two sources of sialic acid (Neu5Ac) to the lactating offspring.
33664475	3	86	from	HMO	475:477	arg1	mice					482:485	mice	482:485	mice	482:485	Here, we investigated the long-term consequences of a selective lactational deprivation of a specific sialylated HMO in mice.
33664475	11	87	theme	reduced	1787:1793	arg1	expression					1795:1804	a site- (PFC) and time-specific (eye-opening) reduced expression	1741:1804	a site- (PFC) and time-specific (eye-opening) reduced expression of genes involved in central nervous system development	1741:1860	These were associated with a site- (PFC) and time-specific (eye-opening) reduced expression of genes involved in central nervous system development.
33664475	3	88	from	mice	482:485	arg1	deprivation					438:448	a selective lactational deprivation	414:448	a selective lactational deprivation of a specific sialylated HMO in mice	414:485	Here, we investigated the long-term consequences of a selective lactational deprivation of a specific sialylated HMO in mice.
33664475	0	89	theme	human	11:15	arg1	milk					17:20	Sialylated human milk	0:20	Sialylated human milk	0:20	Sialylated human milk oligosaccharides program cognitive development through a non-genomic transmission mode.
33664475	9	90	theme	executive	1354:1362	arg1	functions					1364:1372	executive functions	1354:1372	executive functions	1354:1372	To investigate the underlying molecular mechanisms, we assessed gene expression (at eye-opening and in adulthood) in two brain regions mediating executive functions and memory (hippocampus and prefrontal cortex, PFC).
31945798	2	0	with	patients	401:408	arg1	ITP					415:417	ITP	415:417	ITP	415:417	Examining their platelet and immune features, we aimed to detect singular characteristics of patients with ITP who do not respond to any treatment.
31945798	6	1	theme	T-regulator	1050:1060	arg1	subset					1063:1068	a reduced LTreg (Lymphocyte T-regulator) subset	1022:1068	a reduced LTreg (Lymphocyte T-regulator) subset	1022:1068	NR-ITP patients had an increased fraction of naive lymphocyte (L) B cells and a reduced LTreg (Lymphocyte T-regulator) subset.
31945798	10	2	theme	immune	1497:1502	arg1	response					1504:1511	immune response	1497:1511	immune response	1497:1511	The inverse correlation between loss of sialic acid and LTreg count suggests a potential relationship between glycan composition on the platelet surface and immune response, positing terminal sugar moieties of the glycan chains as aetiopathogenic agents in ITP.
31945798	5	3	theme	acid	880:883	arg1	residues					885:892	platelet surface sialic acid residues	856:892	platelet surface sialic acid residues	856:892	Increased loss of platelet surface sialic acid residues was associated with increased platelet apoptosis.
31945798	10	4	theme	terminal	1523:1530	arg1	agents					1587:1592	aetiopathogenic agents	1571:1592	aetiopathogenic agents in ITP	1571:1599	The inverse correlation between loss of sialic acid and LTreg count suggests a potential relationship between glycan composition on the platelet surface and immune response, positing terminal sugar moieties of the glycan chains as aetiopathogenic agents in ITP.
31945798	10	4	theme	terminal	1523:1530	arg1	moieties					1538:1545	terminal sugar moieties	1523:1545	terminal sugar moieties of the glycan chains	1523:1566	The inverse correlation between loss of sialic acid and LTreg count suggests a potential relationship between glycan composition on the platelet surface and immune response, positing terminal sugar moieties of the glycan chains as aetiopathogenic agents in ITP.
31945798	10	5	theme	sialic	1380:1385	arg1	acid					1387:1390	sialic acid	1380:1390	sialic acid	1380:1390	The inverse correlation between loss of sialic acid and LTreg count suggests a potential relationship between glycan composition on the platelet surface and immune response, positing terminal sugar moieties of the glycan chains as aetiopathogenic agents in ITP.
31945798	4	6	theme	acid	823:826	arg1	residues					828:835	sialic acid residues	816:835	sialic acid residues	816:835	Platelets from NR-ITP patients exposed a reduced amount of sialic acid residues.
31945798	10	7	theme	chains	1561:1566	arg1	agents					1587:1592	aetiopathogenic agents	1571:1592	aetiopathogenic agents in ITP	1571:1599	The inverse correlation between loss of sialic acid and LTreg count suggests a potential relationship between glycan composition on the platelet surface and immune response, positing terminal sugar moieties of the glycan chains as aetiopathogenic agents in ITP.
31945798	10	7	theme	chains	1561:1566	arg1	moieties					1538:1545	terminal sugar moieties	1523:1545	terminal sugar moieties of the glycan chains	1523:1566	The inverse correlation between loss of sialic acid and LTreg count suggests a potential relationship between glycan composition on the platelet surface and immune response, positing terminal sugar moieties of the glycan chains as aetiopathogenic agents in ITP.
31945798	2	8	theme	immune	337:342	arg1	features					344:351	their platelet and immune features	318:351	their platelet and immune features	318:351	Examining their platelet and immune features, we aimed to detect singular characteristics of patients with ITP who do not respond to any treatment.
31945798	3	9	dep	included	581:588	arg1	responders					595:604	responders	595:604	responders to agonists of thrombopoietin receptors (TPO-RA), patients who showed no response to first- and second-line treatments (NR-ITP), and healthy controls	595:754	We studied patients with chronic primary ITP who had been without treatment, or untreated (UT-ITP), for at least six months; included were responders to agonists of thrombopoietin receptors (TPO-RA), patients who showed no response to first- and second-line treatments (NR-ITP), and healthy controls.
31945798	3	10	with	patients	467:474	arg1	ITP					497:499	chronic primary ITP	481:499	chronic primary ITP	481:499	We studied patients with chronic primary ITP who had been without treatment, or untreated (UT-ITP), for at least six months; included were responders to agonists of thrombopoietin receptors (TPO-RA), patients who showed no response to first- and second-line treatments (NR-ITP), and healthy controls.
31945798	2	11	theme	platelet	324:331	arg1	features					344:351	their platelet and immune features	318:351	their platelet and immune features	318:351	Examining their platelet and immune features, we aimed to detect singular characteristics of patients with ITP who do not respond to any treatment.
31945798	3	12	theme	healthy	739:745	arg1	controls					747:754	healthy controls	739:754	healthy controls	739:754	We studied patients with chronic primary ITP who had been without treatment, or untreated (UT-ITP), for at least six months; included were responders to agonists of thrombopoietin receptors (TPO-RA), patients who showed no response to first- and second-line treatments (NR-ITP), and healthy controls.
31945798	10	13	theme	aetiopathogenic	1571:1585	arg1	agents					1587:1592	aetiopathogenic agents	1571:1592	aetiopathogenic agents in ITP	1571:1599	The inverse correlation between loss of sialic acid and LTreg count suggests a potential relationship between glycan composition on the platelet surface and immune response, positing terminal sugar moieties of the glycan chains as aetiopathogenic agents in ITP.
31945798	10	13	theme	aetiopathogenic	1571:1585	arg1	moieties					1538:1545	terminal sugar moieties	1523:1545	terminal sugar moieties of the glycan chains	1523:1566	The inverse correlation between loss of sialic acid and LTreg count suggests a potential relationship between glycan composition on the platelet surface and immune response, positing terminal sugar moieties of the glycan chains as aetiopathogenic agents in ITP.
31945798	5	14	theme	residues	885:892	arg1	loss					848:851	Increased loss	838:851	Increased loss of platelet surface sialic acid residues	838:892	Increased loss of platelet surface sialic acid residues was associated with increased platelet apoptosis.
31945798	5	15	theme	platelet	856:863	arg1	residues					885:892	platelet surface sialic acid residues	856:892	platelet surface sialic acid residues	856:892	Increased loss of platelet surface sialic acid residues was associated with increased platelet apoptosis.
31945798	6	16	theme	naive	989:993	arg1	cells					1012:1016	naive lymphocyte (L) B cells	989:1016	naive lymphocyte (L) B cells	989:1016	NR-ITP patients had an increased fraction of naive lymphocyte (L) B cells and a reduced LTreg (Lymphocyte T-regulator) subset.
31945798	10	17	theme	count	1402:1406	arg1	loss					1372:1375	loss	1372:1375	loss of sialic acid and LTreg count	1372:1406	The inverse correlation between loss of sialic acid and LTreg count suggests a potential relationship between glycan composition on the platelet surface and immune response, positing terminal sugar moieties of the glycan chains as aetiopathogenic agents in ITP.
31945798	1	18	from	counts	192:197	arg1	ITP					228:230	ITP	228:230	ITP	228:230	Multifactorial mechanisms leading to diminished platelet counts in immune thrombocytopaenia (ITP) might condition the ability of patients with ITP to respond to treatments.
31945798	1	18	from	counts	192:197	arg1	thrombocytopaenia					209:225	immune thrombocytopaenia	202:225	immune thrombocytopaenia (ITP)	202:231	Multifactorial mechanisms leading to diminished platelet counts in immune thrombocytopaenia (ITP) might condition the ability of patients with ITP to respond to treatments.
31945798	5	19	theme	sialic	873:878	arg1	residues					885:892	platelet surface sialic acid residues	856:892	platelet surface sialic acid residues	856:892	Increased loss of platelet surface sialic acid residues was associated with increased platelet apoptosis.
31945798	0	20	theme	severe	106:111	arg1	dysregulation					120:132	severe immune dysregulation	106:132	severe immune dysregulation	106:132	Platelet and immune characteristics of immune thrombocytopaenia patients non-responsive to therapy reveal severe immune dysregulation.
31945798	7	21	theme	cells	1137:1141	arg1	distribution					1143:1154	an anomalous monocyte and NK (Natural Killer) cells distribution	1091:1154	an anomalous monocyte and NK (Natural Killer) cells distribution	1091:1154	They also presented an anomalous monocyte and NK (Natural Killer) cells distribution.
31945798	0	22	theme	immune	39:44	arg1	patients					64:71	immune thrombocytopaenia patients	39:71	immune thrombocytopaenia patients	39:71	Platelet and immune characteristics of immune thrombocytopaenia patients non-responsive to therapy reveal severe immune dysregulation.
31945798	6	23	theme	LTreg	1032:1036	arg1	subset					1063:1068	a reduced LTreg (Lymphocyte T-regulator) subset	1022:1068	a reduced LTreg (Lymphocyte T-regulator) subset	1022:1068	NR-ITP patients had an increased fraction of naive lymphocyte (L) B cells and a reduced LTreg (Lymphocyte T-regulator) subset.
31945798	7	24	theme	Killer	1129:1134	arg1	cells					1137:1141	NK (Natural Killer) cells	1117:1141	NK (Natural Killer) cells	1117:1141	They also presented an anomalous monocyte and NK (Natural Killer) cells distribution.
31945798	9	25	theme	severe	1288:1293	arg1	deregulation					1295:1306	a severe deregulation	1286:1306	a severe deregulation of the immune system of NR-ITP	1286:1337	In conclusion, our results indicate a severe deregulation of the immune system of NR-ITP.
31945798	5	26	theme	increased	914:922	arg1	apoptosis					933:941	increased platelet apoptosis	914:941	increased platelet apoptosis	914:941	Increased loss of platelet surface sialic acid residues was associated with increased platelet apoptosis.
31945798	0	27	theme	immune	13:18	arg1	characteristics					20:34	immune characteristics	13:34	immune characteristics	13:34	Platelet and immune characteristics of immune thrombocytopaenia patients non-responsive to therapy reveal severe immune dysregulation.
31945798	6	28	theme	cells	1012:1016	arg1	fraction					977:984	an increased fraction	964:984	an increased fraction of naive lymphocyte (L) B cells	964:1016	NR-ITP patients had an increased fraction of naive lymphocyte (L) B cells and a reduced LTreg (Lymphocyte T-regulator) subset.
31945798	6	28	theme	cells	1012:1016	arg1	subset					1063:1068	a reduced LTreg (Lymphocyte T-regulator) subset	1022:1068	a reduced LTreg (Lymphocyte T-regulator) subset	1022:1068	NR-ITP patients had an increased fraction of naive lymphocyte (L) B cells and a reduced LTreg (Lymphocyte T-regulator) subset.
31945798	10	29	theme	glycan	1450:1455	arg1	composition					1457:1467	glycan composition	1450:1467	glycan composition	1450:1467	The inverse correlation between loss of sialic acid and LTreg count suggests a potential relationship between glycan composition on the platelet surface and immune response, positing terminal sugar moieties of the glycan chains as aetiopathogenic agents in ITP.
31945798	3	30	dep	least	563:567	arg1	at					560:561	at	560:561	at	560:561	We studied patients with chronic primary ITP who had been without treatment, or untreated (UT-ITP), for at least six months; included were responders to agonists of thrombopoietin receptors (TPO-RA), patients who showed no response to first- and second-line treatments (NR-ITP), and healthy controls.
31945798	9	31	theme	immune	1315:1320	arg1	system					1322:1327	the immune system	1311:1327	the immune system of NR-ITP	1311:1337	In conclusion, our results indicate a severe deregulation of the immune system of NR-ITP.
31945798	3	32	theme	thrombopoietin	621:634	arg1	receptors					636:644	thrombopoietin receptors	621:644	thrombopoietin receptors (TPO-RA)	621:653	We studied patients with chronic primary ITP who had been without treatment, or untreated (UT-ITP), for at least six months; included were responders to agonists of thrombopoietin receptors (TPO-RA), patients who showed no response to first- and second-line treatments (NR-ITP), and healthy controls.
31945798	3	32	theme	thrombopoietin	621:634	arg1	TPO-RA					647:652	TPO-RA	647:652	TPO-RA	647:652	We studied patients with chronic primary ITP who had been without treatment, or untreated (UT-ITP), for at least six months; included were responders to agonists of thrombopoietin receptors (TPO-RA), patients who showed no response to first- and second-line treatments (NR-ITP), and healthy controls.
31945798	4	33	theme	reduced	798:804	arg1	amount					806:811	a reduced amount	796:811	a reduced amount of sialic acid residues	796:835	Platelets from NR-ITP patients exposed a reduced amount of sialic acid residues.
31945798	4	33	theme	reduced	798:804	arg1	residues					828:835	sialic acid residues	816:835	sialic acid residues	816:835	Platelets from NR-ITP patients exposed a reduced amount of sialic acid residues.
31945798	0	34	theme	thrombocytopaenia	46:62	arg1	patients					64:71	immune thrombocytopaenia patients	39:71	immune thrombocytopaenia patients	39:71	Platelet and immune characteristics of immune thrombocytopaenia patients non-responsive to therapy reveal severe immune dysregulation.
31945798	10	35	theme	platelet	1476:1483	arg1	surface					1485:1491	the platelet surface	1472:1491	the platelet surface	1472:1491	The inverse correlation between loss of sialic acid and LTreg count suggests a potential relationship between glycan composition on the platelet surface and immune response, positing terminal sugar moieties of the glycan chains as aetiopathogenic agents in ITP.
31945798	3	36	theme	patients	656:663	arg1	agonists					609:616	agonists	609:616	agonists of thrombopoietin receptors (TPO-RA), patients who showed no response to first- and second-line treatments (NR-ITP), and healthy controls	609:754	We studied patients with chronic primary ITP who had been without treatment, or untreated (UT-ITP), for at least six months; included were responders to agonists of thrombopoietin receptors (TPO-RA), patients who showed no response to first- and second-line treatments (NR-ITP), and healthy controls.
31945798	3	37	theme	primary	489:495	arg1	ITP					497:499	chronic primary ITP	481:499	chronic primary ITP	481:499	We studied patients with chronic primary ITP who had been without treatment, or untreated (UT-ITP), for at least six months; included were responders to agonists of thrombopoietin receptors (TPO-RA), patients who showed no response to first- and second-line treatments (NR-ITP), and healthy controls.
31945798	1	38	with	patients	264:271	arg1	ITP					278:280	ITP	278:280	ITP	278:280	Multifactorial mechanisms leading to diminished platelet counts in immune thrombocytopaenia (ITP) might condition the ability of patients with ITP to respond to treatments.
31945798	1	39	theme	platelet	183:190	arg1	counts					192:197	diminished platelet counts	172:197	diminished platelet counts in immune thrombocytopaenia (ITP)	172:231	Multifactorial mechanisms leading to diminished platelet counts in immune thrombocytopaenia (ITP) might condition the ability of patients with ITP to respond to treatments.
31945798	4	40	theme	NR-ITP	772:777	arg1	patients					779:786	NR-ITP patients	772:786	NR-ITP patients	772:786	Platelets from NR-ITP patients exposed a reduced amount of sialic acid residues.
31945798	1	41	theme	patients	264:271	arg1	ability					253:259	the ability	249:259	the ability of patients with ITP to respond to treatments	249:305	Multifactorial mechanisms leading to diminished platelet counts in immune thrombocytopaenia (ITP) might condition the ability of patients with ITP to respond to treatments.
31945798	2	42	theme	singular	373:380	arg1	characteristics					382:396	singular characteristics	373:396	singular characteristics of patients with ITP who do not respond to any treatment	373:453	Examining their platelet and immune features, we aimed to detect singular characteristics of patients with ITP who do not respond to any treatment.
31945798	4	43	theme	residues	828:835	arg1	amount					806:811	a reduced amount	796:811	a reduced amount of sialic acid residues	796:835	Platelets from NR-ITP patients exposed a reduced amount of sialic acid residues.
31945798	4	43	theme	residues	828:835	arg1	residues					828:835	sialic acid residues	816:835	sialic acid residues	816:835	Platelets from NR-ITP patients exposed a reduced amount of sialic acid residues.
31945798	6	44	theme	Lymphocyte	1039:1048	arg1	subset					1063:1068	a reduced LTreg (Lymphocyte T-regulator) subset	1022:1068	a reduced LTreg (Lymphocyte T-regulator) subset	1022:1068	NR-ITP patients had an increased fraction of naive lymphocyte (L) B cells and a reduced LTreg (Lymphocyte T-regulator) subset.
31945798	10	45	theme	inverse	1344:1350	arg1	correlation					1352:1362	The inverse correlation	1340:1362	The inverse correlation between loss of sialic acid and LTreg count	1340:1406	The inverse correlation between loss of sialic acid and LTreg count suggests a potential relationship between glycan composition on the platelet surface and immune response, positing terminal sugar moieties of the glycan chains as aetiopathogenic agents in ITP.
31945798	10	46	from	agents	1587:1592	arg1	ITP					1597:1599	ITP	1597:1599	ITP	1597:1599	The inverse correlation between loss of sialic acid and LTreg count suggests a potential relationship between glycan composition on the platelet surface and immune response, positing terminal sugar moieties of the glycan chains as aetiopathogenic agents in ITP.
31945798	10	47	theme	sugar	1532:1536	arg1	agents					1587:1592	aetiopathogenic agents	1571:1592	aetiopathogenic agents in ITP	1571:1599	The inverse correlation between loss of sialic acid and LTreg count suggests a potential relationship between glycan composition on the platelet surface and immune response, positing terminal sugar moieties of the glycan chains as aetiopathogenic agents in ITP.
31945798	10	47	theme	sugar	1532:1536	arg1	moieties					1538:1545	terminal sugar moieties	1523:1545	terminal sugar moieties of the glycan chains	1523:1566	The inverse correlation between loss of sialic acid and LTreg count suggests a potential relationship between glycan composition on the platelet surface and immune response, positing terminal sugar moieties of the glycan chains as aetiopathogenic agents in ITP.
31945798	3	48	theme	second-line	702:712	arg1	NR-ITP					726:731	NR-ITP	726:731	NR-ITP	726:731	We studied patients with chronic primary ITP who had been without treatment, or untreated (UT-ITP), for at least six months; included were responders to agonists of thrombopoietin receptors (TPO-RA), patients who showed no response to first- and second-line treatments (NR-ITP), and healthy controls.
31945798	3	48	theme	second-line	702:712	arg1	treatments					714:723	first- and second-line treatments	691:723	first- and second-line treatments (NR-ITP)	691:732	We studied patients with chronic primary ITP who had been without treatment, or untreated (UT-ITP), for at least six months; included were responders to agonists of thrombopoietin receptors (TPO-RA), patients who showed no response to first- and second-line treatments (NR-ITP), and healthy controls.
31945798	1	49	theme	immune	202:207	arg1	ITP					228:230	ITP	228:230	ITP	228:230	Multifactorial mechanisms leading to diminished platelet counts in immune thrombocytopaenia (ITP) might condition the ability of patients with ITP to respond to treatments.
31945798	1	49	theme	immune	202:207	arg1	thrombocytopaenia					209:225	immune thrombocytopaenia	202:225	immune thrombocytopaenia (ITP)	202:231	Multifactorial mechanisms leading to diminished platelet counts in immune thrombocytopaenia (ITP) might condition the ability of patients with ITP to respond to treatments.
31945798	7	50	theme	anomalous	1094:1102	arg1	distribution					1143:1154	an anomalous monocyte and NK (Natural Killer) cells distribution	1091:1154	an anomalous monocyte and NK (Natural Killer) cells distribution	1091:1154	They also presented an anomalous monocyte and NK (Natural Killer) cells distribution.
31945798	3	51	theme	first-	691:696	arg1	NR-ITP					726:731	NR-ITP	726:731	NR-ITP	726:731	We studied patients with chronic primary ITP who had been without treatment, or untreated (UT-ITP), for at least six months; included were responders to agonists of thrombopoietin receptors (TPO-RA), patients who showed no response to first- and second-line treatments (NR-ITP), and healthy controls.
31945798	3	51	theme	first-	691:696	arg1	treatments					714:723	first- and second-line treatments	691:723	first- and second-line treatments (NR-ITP)	691:732	We studied patients with chronic primary ITP who had been without treatment, or untreated (UT-ITP), for at least six months; included were responders to agonists of thrombopoietin receptors (TPO-RA), patients who showed no response to first- and second-line treatments (NR-ITP), and healthy controls.
31945798	5	52	theme	Increased	838:846	arg1	loss					848:851	Increased loss	838:851	Increased loss of platelet surface sialic acid residues	838:892	Increased loss of platelet surface sialic acid residues was associated with increased platelet apoptosis.
31945798	4	53	theme	sialic	816:821	arg1	residues					828:835	sialic acid residues	816:835	sialic acid residues	816:835	Platelets from NR-ITP patients exposed a reduced amount of sialic acid residues.
31945798	8	54	theme	immune	1202:1207	arg1	homeostasis					1209:1219	an immune homeostasis	1199:1219	an immune homeostasis similar to healthy controls	1199:1247	TPO-RA-treated patients seemed to recover an immune homeostasis similar to healthy controls.
31945798	10	55	theme	glycan	1554:1559	arg1	chains					1561:1566	the glycan chains	1550:1566	the glycan chains	1550:1566	The inverse correlation between loss of sialic acid and LTreg count suggests a potential relationship between glycan composition on the platelet surface and immune response, positing terminal sugar moieties of the glycan chains as aetiopathogenic agents in ITP.
31945798	6	56	theme	NR-ITP	944:949	arg1	patients					951:958	NR-ITP patients	944:958	NR-ITP patients	944:958	NR-ITP patients had an increased fraction of naive lymphocyte (L) B cells and a reduced LTreg (Lymphocyte T-regulator) subset.
31945798	8	57	theme	similar	1221:1227	arg1	homeostasis					1209:1219	an immune homeostasis	1199:1219	an immune homeostasis similar to healthy controls	1199:1247	TPO-RA-treated patients seemed to recover an immune homeostasis similar to healthy controls.
31945798	8	58	theme	TPO-RA-treated	1157:1170	arg1	patients					1172:1179	TPO-RA-treated patients	1157:1179	TPO-RA-treated patients	1157:1179	TPO-RA-treated patients seemed to recover an immune homeostasis similar to healthy controls.
31945798	8	59	theme	healthy	1232:1238	arg1	controls					1240:1247	healthy controls	1232:1247	healthy controls	1232:1247	TPO-RA-treated patients seemed to recover an immune homeostasis similar to healthy controls.
31945798	3	60	theme	controls	747:754	arg1	agonists					609:616	agonists	609:616	agonists of thrombopoietin receptors (TPO-RA), patients who showed no response to first- and second-line treatments (NR-ITP), and healthy controls	609:754	We studied patients with chronic primary ITP who had been without treatment, or untreated (UT-ITP), for at least six months; included were responders to agonists of thrombopoietin receptors (TPO-RA), patients who showed no response to first- and second-line treatments (NR-ITP), and healthy controls.
31945798	3	61	theme	least	563:567	arg1	six months					569:578	at least six months	560:578	at least six months	560:578	We studied patients with chronic primary ITP who had been without treatment, or untreated (UT-ITP), for at least six months; included were responders to agonists of thrombopoietin receptors (TPO-RA), patients who showed no response to first- and second-line treatments (NR-ITP), and healthy controls.
31945798	1	62	theme	Multifactorial	135:148	arg1	mechanisms					150:159	Multifactorial mechanisms	135:159	Multifactorial mechanisms leading to diminished platelet counts in immune thrombocytopaenia (ITP)	135:231	Multifactorial mechanisms leading to diminished platelet counts in immune thrombocytopaenia (ITP) might condition the ability of patients with ITP to respond to treatments.
31945798	4	63	from	patients	779:786	arg1	Platelets					757:765	Platelets	757:765	Platelets from NR-ITP patients	757:786	Platelets from NR-ITP patients exposed a reduced amount of sialic acid residues.
31945798	6	64	theme	L	1007:1007	arg1	cells					1012:1016	naive lymphocyte (L) B cells	989:1016	naive lymphocyte (L) B cells	989:1016	NR-ITP patients had an increased fraction of naive lymphocyte (L) B cells and a reduced LTreg (Lymphocyte T-regulator) subset.
31945798	1	65	theme	diminished	172:181	arg1	counts					192:197	diminished platelet counts	172:197	diminished platelet counts in immune thrombocytopaenia (ITP)	172:231	Multifactorial mechanisms leading to diminished platelet counts in immune thrombocytopaenia (ITP) might condition the ability of patients with ITP to respond to treatments.
31945798	6	66	theme	lymphocyte	995:1004	arg1	cells					1012:1016	naive lymphocyte (L) B cells	989:1016	naive lymphocyte (L) B cells	989:1016	NR-ITP patients had an increased fraction of naive lymphocyte (L) B cells and a reduced LTreg (Lymphocyte T-regulator) subset.
31945798	5	67	theme	surface	865:871	arg1	residues					885:892	platelet surface sialic acid residues	856:892	platelet surface sialic acid residues	856:892	Increased loss of platelet surface sialic acid residues was associated with increased platelet apoptosis.
31945798	10	68	theme	LTreg	1396:1400	arg1	count					1402:1406	LTreg count	1396:1406	LTreg count	1396:1406	The inverse correlation between loss of sialic acid and LTreg count suggests a potential relationship between glycan composition on the platelet surface and immune response, positing terminal sugar moieties of the glycan chains as aetiopathogenic agents in ITP.
31945798	0	69	theme	immune	113:118	arg1	dysregulation					120:132	severe immune dysregulation	106:132	severe immune dysregulation	106:132	Platelet and immune characteristics of immune thrombocytopaenia patients non-responsive to therapy reveal severe immune dysregulation.
31945798	6	70	theme	increased	967:975	arg1	fraction					977:984	an increased fraction	964:984	an increased fraction of naive lymphocyte (L) B cells	964:1016	NR-ITP patients had an increased fraction of naive lymphocyte (L) B cells and a reduced LTreg (Lymphocyte T-regulator) subset.
31945798	10	71	theme	acid	1387:1390	arg1	loss					1372:1375	loss	1372:1375	loss of sialic acid and LTreg count	1372:1406	The inverse correlation between loss of sialic acid and LTreg count suggests a potential relationship between glycan composition on the platelet surface and immune response, positing terminal sugar moieties of the glycan chains as aetiopathogenic agents in ITP.
31945798	10	72	theme	potential	1419:1427	arg1	relationship					1429:1440	a potential relationship	1417:1440	a potential relationship between glycan composition	1417:1467	The inverse correlation between loss of sialic acid and LTreg count suggests a potential relationship between glycan composition on the platelet surface and immune response, positing terminal sugar moieties of the glycan chains as aetiopathogenic agents in ITP.
31945798	7	73	theme	Natural	1121:1127	arg1	Killer					1129:1134	Natural Killer	1121:1134	NK (Natural Killer) cells	1117:1141	They also presented an anomalous monocyte and NK (Natural Killer) cells distribution.
31945798	6	74	theme	reduced	1024:1030	arg1	subset					1063:1068	a reduced LTreg (Lymphocyte T-regulator) subset	1022:1068	a reduced LTreg (Lymphocyte T-regulator) subset	1022:1068	NR-ITP patients had an increased fraction of naive lymphocyte (L) B cells and a reduced LTreg (Lymphocyte T-regulator) subset.
31945798	2	75	theme	patients	401:408	arg1	characteristics					382:396	singular characteristics	373:396	singular characteristics of patients with ITP who do not respond to any treatment	373:453	Examining their platelet and immune features, we aimed to detect singular characteristics of patients with ITP who do not respond to any treatment.
31945798	7	76	theme	NK	1117:1118	arg1	cells					1137:1141	NK (Natural Killer) cells	1117:1141	NK (Natural Killer) cells	1117:1141	They also presented an anomalous monocyte and NK (Natural Killer) cells distribution.
31945798	5	77	theme	platelet	924:931	arg1	apoptosis					933:941	increased platelet apoptosis	914:941	increased platelet apoptosis	914:941	Increased loss of platelet surface sialic acid residues was associated with increased platelet apoptosis.
31945798	3	78	theme	receptors	636:644	arg1	agonists					609:616	agonists	609:616	agonists of thrombopoietin receptors (TPO-RA), patients who showed no response to first- and second-line treatments (NR-ITP), and healthy controls	609:754	We studied patients with chronic primary ITP who had been without treatment, or untreated (UT-ITP), for at least six months; included were responders to agonists of thrombopoietin receptors (TPO-RA), patients who showed no response to first- and second-line treatments (NR-ITP), and healthy controls.
31945798	3	79	theme	chronic	481:487	arg1	ITP					497:499	chronic primary ITP	481:499	chronic primary ITP	481:499	We studied patients with chronic primary ITP who had been without treatment, or untreated (UT-ITP), for at least six months; included were responders to agonists of thrombopoietin receptors (TPO-RA), patients who showed no response to first- and second-line treatments (NR-ITP), and healthy controls.
31945798	7	80	theme	monocyte	1104:1111	arg1	distribution					1143:1154	an anomalous monocyte and NK (Natural Killer) cells distribution	1091:1154	an anomalous monocyte and NK (Natural Killer) cells distribution	1091:1154	They also presented an anomalous monocyte and NK (Natural Killer) cells distribution.
31945798	6	81	theme	B	1010:1010	arg1	cells					1012:1016	naive lymphocyte (L) B cells	989:1016	naive lymphocyte (L) B cells	989:1016	NR-ITP patients had an increased fraction of naive lymphocyte (L) B cells and a reduced LTreg (Lymphocyte T-regulator) subset.
31945798	0	82	theme	patients	64:71	arg1	Platelet					0:7	Platelet	0:7	Platelet	0:7	Platelet and immune characteristics of immune thrombocytopaenia patients non-responsive to therapy reveal severe immune dysregulation.
31945798	0	82	theme	patients	64:71	arg1	characteristics					20:34	immune characteristics	13:34	immune characteristics	13:34	Platelet and immune characteristics of immune thrombocytopaenia patients non-responsive to therapy reveal severe immune dysregulation.
31945798	9	83	theme	system	1322:1327	arg1	deregulation					1295:1306	a severe deregulation	1286:1306	a severe deregulation of the immune system of NR-ITP	1286:1337	In conclusion, our results indicate a severe deregulation of the immune system of NR-ITP.
31945798	6	84	contain	had	960:962	arg2	subset					1063:1068	a reduced LTreg (Lymphocyte T-regulator) subset	1022:1068	a reduced LTreg (Lymphocyte T-regulator) subset	1022:1068	NR-ITP patients had an increased fraction of naive lymphocyte (L) B cells and a reduced LTreg (Lymphocyte T-regulator) subset.
31945798	6	84	contain	had	960:962	arg1	patients					951:958	NR-ITP patients	944:958	NR-ITP patients	944:958	NR-ITP patients had an increased fraction of naive lymphocyte (L) B cells and a reduced LTreg (Lymphocyte T-regulator) subset.
31945798	6	84	contain	had	960:962	arg2	fraction					977:984	an increased fraction	964:984	an increased fraction of naive lymphocyte (L) B cells	964:1016	NR-ITP patients had an increased fraction of naive lymphocyte (L) B cells and a reduced LTreg (Lymphocyte T-regulator) subset.
31945798	9	85	theme	NR-ITP	1332:1337	arg1	system					1322:1327	the immune system	1311:1327	the immune system of NR-ITP	1311:1337	In conclusion, our results indicate a severe deregulation of the immune system of NR-ITP.
34823852	0	0	theme	enhanced	88:95	arg1	removal					97:103	enhanced removal	88:103	enhanced removal of oxytetracycline	88:122	Cyclodextrin modified green synthesized graphene oxide@iron nanoparticle composites for enhanced removal of oxytetracycline.
34823852	5	1	theme	diffraction	688:698	arg1	analysis					700:707	Raman and X-ray diffraction analysis	672:707	analysis	700:707	Raman and X-ray diffraction analysis showed that GO acted as a carrier to support Fe NPs within the grafted cyclodextrin, where GO also participated in the removal process.
34823852	4	2	theme	@	631:631	arg1	NPs					635:637	GO@Fe NPs	629:637	GO@Fe NPs	629:637	Characterization techniques were applied to better understand how CDs impact the structure of GO@Fe NPs and improve removal performance.
34823852	6	3	theme	high	929:932	arg1	61.7 m2 · g-1					948:960	61.7 m2 · g-1	948:960	61.7 m2 · g-1	948:960	Cyclodextrin modified GO@Fe NPs had relatively small particle sizes (15 nm), with a high surface area (61.7 m2 · g-1).
34823852	6	3	theme	high	929:932	arg1	area					942:945	a high surface area	927:945	a high surface area (61.7 m2 · g-1)	927:961	Cyclodextrin modified GO@Fe NPs had relatively small particle sizes (15 nm), with a high surface area (61.7 m2 · g-1).
34823852	2	4	theme	hydrochloride	355:367	arg1	absorption					325:334	the absorption	321:334	the absorption of oxytetracycline hydrochloride (OTC)	321:373	Here cyclodextrins (CDs) were successfully used to modify graphene-based iron nanoparticles (GO@Fe NPs) to enhance the absorption of oxytetracycline hydrochloride (OTC).
34823852	5	5	theme	Raman	672:676	arg1	analysis					700:707	Raman and X-ray diffraction analysis	672:707	analysis	700:707	Raman and X-ray diffraction analysis showed that GO acted as a carrier to support Fe NPs within the grafted cyclodextrin, where GO also participated in the removal process.
34823852	7	6	theme	green	1122:1126	arg1	synthesis					1128:1136	green synthesis	1122:1136	green synthesis	1122:1136	X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy suggested that cyclodextrin acted as both a stabilizing and capping agent during green synthesis, which could protect the reactivity of Fe NPs and simultaneously reduce aggregation.
34823852	6	7	dep	modified	858:865	arg1	GO					867:868	GO	867:868	GO@Fe NPs	867:875	Cyclodextrin modified GO@Fe NPs had relatively small particle sizes (15 nm), with a high surface area (61.7 m2 · g-1).
34823852	2	8	theme	oxytetracycline	339:353	arg1	hydrochloride					355:367	oxytetracycline hydrochloride	339:367	oxytetracycline hydrochloride (OTC)	339:373	Here cyclodextrins (CDs) were successfully used to modify graphene-based iron nanoparticles (GO@Fe NPs) to enhance the absorption of oxytetracycline hydrochloride (OTC).
34823852	2	8	theme	oxytetracycline	339:353	arg1	OTC					370:372	OTC	370:372	OTC	370:372	Here cyclodextrins (CDs) were successfully used to modify graphene-based iron nanoparticles (GO@Fe NPs) to enhance the absorption of oxytetracycline hydrochloride (OTC).
34823852	6	9	theme	particle	898:905	arg1	15 nm					914:918	15 nm	914:918	15 nm	914:918	Cyclodextrin modified GO@Fe NPs had relatively small particle sizes (15 nm), with a high surface area (61.7 m2 · g-1).
34823852	6	9	theme	particle	898:905	arg1	sizes					907:911	relatively small particle sizes	881:911	relatively small particle sizes (15 nm)	881:919	Cyclodextrin modified GO@Fe NPs had relatively small particle sizes (15 nm), with a high surface area (61.7 m2 · g-1).
34823852	7	10	theme	capping	1101:1107	arg1	cyclodextrin					1056:1067	cyclodextrin	1056:1067	cyclodextrin	1056:1067	X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy suggested that cyclodextrin acted as both a stabilizing and capping agent during green synthesis, which could protect the reactivity of Fe NPs and simultaneously reduce aggregation.
34823852	7	10	theme	capping	1101:1107	arg1	agent					1109:1113	both a stabilizing and capping agent	1078:1113	agent	1109:1113	X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy suggested that cyclodextrin acted as both a stabilizing and capping agent during green synthesis, which could protect the reactivity of Fe NPs and simultaneously reduce aggregation.
34823852	7	11	theme	photoelectron	970:982	arg1	spectroscopy					984:995	X-ray photoelectron spectroscopy	964:995	X-ray photoelectron spectroscopy	964:995	X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy suggested that cyclodextrin acted as both a stabilizing and capping agent during green synthesis, which could protect the reactivity of Fe NPs and simultaneously reduce aggregation.
34823852	2	12	theme	@	301:301	arg1	NPs					305:307	GO@Fe NPs	299:307	GO@Fe NPs	299:307	Here cyclodextrins (CDs) were successfully used to modify graphene-based iron nanoparticles (GO@Fe NPs) to enhance the absorption of oxytetracycline hydrochloride (OTC).
34823852	2	12	theme	@	301:301	arg1	nanoparticles					284:296	graphene-based iron nanoparticles	264:296	graphene-based iron nanoparticles (GO@Fe NPs) to enhance the absorption of oxytetracycline hydrochloride (OTC)	264:373	Here cyclodextrins (CDs) were successfully used to modify graphene-based iron nanoparticles (GO@Fe NPs) to enhance the absorption of oxytetracycline hydrochloride (OTC).
34823852	5	13	dep	GO	800:801	arg1	participated					808:819	participated	808:819	GO also participated in the removal process	800:842	Raman and X-ray diffraction analysis showed that GO acted as a carrier to support Fe NPs within the grafted cyclodextrin, where GO also participated in the removal process.
34823852	6	14	theme	small	892:896	arg1	15 nm					914:918	15 nm	914:918	15 nm	914:918	Cyclodextrin modified GO@Fe NPs had relatively small particle sizes (15 nm), with a high surface area (61.7 m2 · g-1).
34823852	6	14	theme	small	892:896	arg1	sizes					907:911	relatively small particle sizes	881:911	relatively small particle sizes (15 nm)	881:919	Cyclodextrin modified GO@Fe NPs had relatively small particle sizes (15 nm), with a high surface area (61.7 m2 · g-1).
34823852	2	15	theme	GO	299:300	arg1	NPs					305:307	GO@Fe NPs	299:307	GO@Fe NPs	299:307	Here cyclodextrins (CDs) were successfully used to modify graphene-based iron nanoparticles (GO@Fe NPs) to enhance the absorption of oxytetracycline hydrochloride (OTC).
34823852	2	15	theme	GO	299:300	arg1	nanoparticles					284:296	graphene-based iron nanoparticles	264:296	graphene-based iron nanoparticles (GO@Fe NPs) to enhance the absorption of oxytetracycline hydrochloride (OTC)	264:373	Here cyclodextrins (CDs) were successfully used to modify graphene-based iron nanoparticles (GO@Fe NPs) to enhance the absorption of oxytetracycline hydrochloride (OTC).
34823852	3	16	theme	NPs > αCD-GO	445:456	arg1	NPs					473:475	γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	419:475	the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	408:475	The removal of OTC decreased in the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs, with better performance than that of bare GO and Fe NPs.
34823852	4	17	theme	GO	629:630	arg1	NPs					635:637	GO@Fe NPs	629:637	GO@Fe NPs	629:637	Characterization techniques were applied to better understand how CDs impact the structure of GO@Fe NPs and improve removal performance.
34823852	4	18	theme	NPs	635:637	arg1	structure					616:624	the structure	612:624	the structure of GO@Fe NPs	612:637	Characterization techniques were applied to better understand how CDs impact the structure of GO@Fe NPs and improve removal performance.
34823852	5	19	theme	Fe	754:755	arg1	NPs					757:759	Fe NPs	754:759	Fe NPs within the grafted cyclodextrin	754:791	Raman and X-ray diffraction analysis showed that GO acted as a carrier to support Fe NPs within the grafted cyclodextrin, where GO also participated in the removal process.
34823852	3	20	theme	@	441:441	arg1	NPs					473:475	γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	419:475	the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	408:475	The removal of OTC decreased in the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs, with better performance than that of bare GO and Fe NPs.
34823852	8	21	theme	modified	1272:1279	arg1	composites					1281:1290	cyclodextrins modified composites	1258:1290	cyclodextrins modified composites	1258:1290	A potential synthesis mechanism of cyclodextrins modified composites was also proposed, and subsequent wastewater testing indicated that γCD-GO@Fe NPs had high potential for practical applications.
34823852	7	22	dep	transform	1009:1017	arg1	infrared					1019:1026	infrared	1019:1026	transform infrared spectroscopy	1009:1039	X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy suggested that cyclodextrin acted as both a stabilizing and capping agent during green synthesis, which could protect the reactivity of Fe NPs and simultaneously reduce aggregation.
34823852	1	23	theme	potential	175:183	arg1	risks					199:203	potential environmental risks	175:203	potential environmental risks	175:203	The presence of residual antibiotics will lead to potential environmental risks.
34823852	0	24	theme	oxytetracycline	108:122	arg1	removal					97:103	enhanced removal	88:103	enhanced removal of oxytetracycline	88:122	Cyclodextrin modified green synthesized graphene oxide@iron nanoparticle composites for enhanced removal of oxytetracycline.
34823852	8	25	theme	cyclodextrins	1258:1270	arg1	composites					1281:1290	cyclodextrins modified composites	1258:1290	cyclodextrins modified composites	1258:1290	A potential synthesis mechanism of cyclodextrins modified composites was also proposed, and subsequent wastewater testing indicated that γCD-GO@Fe NPs had high potential for practical applications.
34823852	1	26	theme	environmental	185:197	arg1	risks					199:203	potential environmental risks	175:203	potential environmental risks	175:203	The presence of residual antibiotics will lead to potential environmental risks.
34823852	8	27	contain	had	1374:1376	arg1	NPs					1370:1372	γCD-GO@Fe NPs	1360:1372	γCD-GO@Fe NPs	1360:1372	A potential synthesis mechanism of cyclodextrins modified composites was also proposed, and subsequent wastewater testing indicated that γCD-GO@Fe NPs had high potential for practical applications.
34823852	8	27	contain	had	1374:1376	arg2	potential					1383:1391	high potential	1378:1391	high potential for practical applications	1378:1418	A potential synthesis mechanism of cyclodextrins modified composites was also proposed, and subsequent wastewater testing indicated that γCD-GO@Fe NPs had high potential for practical applications.
34823852	6	28	theme	@	869:869	arg1	NPs					873:875	@Fe NPs	869:875	@Fe NPs	869:875	Cyclodextrin modified GO@Fe NPs had relatively small particle sizes (15 nm), with a high surface area (61.7 m2 · g-1).
34823852	3	29	theme	OTC	391:393	arg1	removal					380:386	The removal	376:386	The removal of OTC	376:393	The removal of OTC decreased in the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs, with better performance than that of bare GO and Fe NPs.
34823852	7	30	dep	Fourier	1001:1007	arg1	transform					1009:1017	transform	1009:1017	transform infrared spectroscopy	1009:1039	X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy suggested that cyclodextrin acted as both a stabilizing and capping agent during green synthesis, which could protect the reactivity of Fe NPs and simultaneously reduce aggregation.
34823852	7	31	theme	X-ray	964:968	arg1	spectroscopy					984:995	X-ray photoelectron spectroscopy	964:995	X-ray photoelectron spectroscopy	964:995	X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy suggested that cyclodextrin acted as both a stabilizing and capping agent during green synthesis, which could protect the reactivity of Fe NPs and simultaneously reduce aggregation.
34823852	4	32	theme	removal	651:657	arg1	performance					659:669	removal performance	651:669	removal performance	651:669	Characterization techniques were applied to better understand how CDs impact the structure of GO@Fe NPs and improve removal performance.
34823852	0	33	theme	synthesized	28:38	arg1	composites					73:82	green synthesized graphene oxide@iron nanoparticle composites	22:82	green synthesized graphene oxide@iron nanoparticle composites for enhanced removal of oxytetracycline	22:122	Cyclodextrin modified green synthesized graphene oxide@iron nanoparticle composites for enhanced removal of oxytetracycline.
34823852	8	34	theme	subsequent	1315:1324	arg1	testing					1337:1343	subsequent wastewater testing	1315:1343	subsequent wastewater testing	1315:1343	A potential synthesis mechanism of cyclodextrins modified composites was also proposed, and subsequent wastewater testing indicated that γCD-GO@Fe NPs had high potential for practical applications.
34823852	8	35	theme	potential	1225:1233	arg1	mechanism					1245:1253	A potential synthesis mechanism	1223:1253	A potential synthesis mechanism of cyclodextrins modified composites	1223:1290	A potential synthesis mechanism of cyclodextrins modified composites was also proposed, and subsequent wastewater testing indicated that γCD-GO@Fe NPs had high potential for practical applications.
34823852	3	36	theme	NPs > βCD-GO	429:440	arg1	NPs					473:475	γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	419:475	the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	408:475	The removal of OTC decreased in the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs, with better performance than that of bare GO and Fe NPs.
34823852	0	37	theme	green	22:26	arg1	composites					73:82	green synthesized graphene oxide@iron nanoparticle composites	22:82	green synthesized graphene oxide@iron nanoparticle composites for enhanced removal of oxytetracycline	22:122	Cyclodextrin modified green synthesized graphene oxide@iron nanoparticle composites for enhanced removal of oxytetracycline.
34823852	6	38	theme	Fe	870:871	arg1	NPs					873:875	@Fe NPs	869:875	@Fe NPs	869:875	Cyclodextrin modified GO@Fe NPs had relatively small particle sizes (15 nm), with a high surface area (61.7 m2 · g-1).
34823852	8	39	theme	Fe	1367:1368	arg1	NPs					1370:1372	γCD-GO@Fe NPs	1360:1372	γCD-GO@Fe NPs	1360:1372	A potential synthesis mechanism of cyclodextrins modified composites was also proposed, and subsequent wastewater testing indicated that γCD-GO@Fe NPs had high potential for practical applications.
34823852	2	40	theme	Fe	302:303	arg1	NPs					305:307	GO@Fe NPs	299:307	GO@Fe NPs	299:307	Here cyclodextrins (CDs) were successfully used to modify graphene-based iron nanoparticles (GO@Fe NPs) to enhance the absorption of oxytetracycline hydrochloride (OTC).
34823852	2	40	theme	Fe	302:303	arg1	nanoparticles					284:296	graphene-based iron nanoparticles	264:296	graphene-based iron nanoparticles (GO@Fe NPs) to enhance the absorption of oxytetracycline hydrochloride (OTC)	264:373	Here cyclodextrins (CDs) were successfully used to modify graphene-based iron nanoparticles (GO@Fe NPs) to enhance the absorption of oxytetracycline hydrochloride (OTC).
34823852	3	41	theme	Fe	470:471	arg1	NPs					473:475	γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	419:475	the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	408:475	The removal of OTC decreased in the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs, with better performance than that of bare GO and Fe NPs.
34823852	0	42	theme	oxide	49:53	arg1	composites					73:82	green synthesized graphene oxide@iron nanoparticle composites	22:82	green synthesized graphene oxide@iron nanoparticle composites for enhanced removal of oxytetracycline	22:122	Cyclodextrin modified green synthesized graphene oxide@iron nanoparticle composites for enhanced removal of oxytetracycline.
34823852	4	43	theme	Fe	632:633	arg1	NPs					635:637	GO@Fe NPs	629:637	GO@Fe NPs	629:637	Characterization techniques were applied to better understand how CDs impact the structure of GO@Fe NPs and improve removal performance.
34823852	7	44	theme	stabilizing	1085:1095	arg1	cyclodextrin					1056:1067	cyclodextrin	1056:1067	cyclodextrin	1056:1067	X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy suggested that cyclodextrin acted as both a stabilizing and capping agent during green synthesis, which could protect the reactivity of Fe NPs and simultaneously reduce aggregation.
34823852	7	44	theme	stabilizing	1085:1095	arg1	agent					1109:1113	both a stabilizing and capping agent	1078:1113	agent	1109:1113	X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy suggested that cyclodextrin acted as both a stabilizing and capping agent during green synthesis, which could protect the reactivity of Fe NPs and simultaneously reduce aggregation.
34823852	8	45	theme	@	1366:1366	arg1	NPs					1370:1372	γCD-GO@Fe NPs	1360:1372	γCD-GO@Fe NPs	1360:1372	A potential synthesis mechanism of cyclodextrins modified composites was also proposed, and subsequent wastewater testing indicated that γCD-GO@Fe NPs had high potential for practical applications.
34823852	3	46	theme	Fe	527:528	arg1	NPs					530:532	Fe NPs	527:532	Fe NPs	527:532	The removal of OTC decreased in the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs, with better performance than that of bare GO and Fe NPs.
34823852	2	47	used	used	249:252	arg2	CDs					226:228	CDs	226:228	CDs	226:228	Here cyclodextrins (CDs) were successfully used to modify graphene-based iron nanoparticles (GO@Fe NPs) to enhance the absorption of oxytetracycline hydrochloride (OTC).
34823852	2	47	used	used	249:252	arg2	cyclodextrins					211:223	cyclodextrins	211:223	cyclodextrins (CDs)	211:229	Here cyclodextrins (CDs) were successfully used to modify graphene-based iron nanoparticles (GO@Fe NPs) to enhance the absorption of oxytetracycline hydrochloride (OTC).
34823852	3	48	theme	@	425:425	arg1	NPs					473:475	γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	419:475	the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	408:475	The removal of OTC decreased in the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs, with better performance than that of bare GO and Fe NPs.
34823852	0	49	theme	graphene	40:47	arg1	composites					73:82	green synthesized graphene oxide@iron nanoparticle composites	22:82	green synthesized graphene oxide@iron nanoparticle composites for enhanced removal of oxytetracycline	22:122	Cyclodextrin modified green synthesized graphene oxide@iron nanoparticle composites for enhanced removal of oxytetracycline.
34823852	5	50	theme	grafted	772:778	arg1	cyclodextrin					780:791	the grafted cyclodextrin	768:791	the grafted cyclodextrin	768:791	Raman and X-ray diffraction analysis showed that GO acted as a carrier to support Fe NPs within the grafted cyclodextrin, where GO also participated in the removal process.
34823852	3	51	dep	order	412:416	arg1	NPs					473:475	γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	419:475	the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	408:475	The removal of OTC decreased in the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs, with better performance than that of bare GO and Fe NPs.
34823852	3	52	theme	Fe	426:427	arg1	NPs					473:475	γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	419:475	the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	408:475	The removal of OTC decreased in the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs, with better performance than that of bare GO and Fe NPs.
34823852	0	53	theme	iron	55:58	arg1	composites					73:82	green synthesized graphene oxide@iron nanoparticle composites	22:82	green synthesized graphene oxide@iron nanoparticle composites for enhanced removal of oxytetracycline	22:122	Cyclodextrin modified green synthesized graphene oxide@iron nanoparticle composites for enhanced removal of oxytetracycline.
34823852	3	54	theme	NPs > GO	461:468	arg1	NPs					473:475	γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	419:475	the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	408:475	The removal of OTC decreased in the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs, with better performance than that of bare GO and Fe NPs.
34823852	5	55	theme	X-ray	682:686	arg1	analysis					700:707	Raman and X-ray diffraction analysis	672:707	analysis	700:707	Raman and X-ray diffraction analysis showed that GO acted as a carrier to support Fe NPs within the grafted cyclodextrin, where GO also participated in the removal process.
34823852	8	56	theme	wastewater	1326:1335	arg1	testing					1337:1343	subsequent wastewater testing	1315:1343	subsequent wastewater testing	1315:1343	A potential synthesis mechanism of cyclodextrins modified composites was also proposed, and subsequent wastewater testing indicated that γCD-GO@Fe NPs had high potential for practical applications.
34823852	8	57	theme	composites	1281:1290	arg1	mechanism					1245:1253	A potential synthesis mechanism	1223:1253	A potential synthesis mechanism of cyclodextrins modified composites	1223:1290	A potential synthesis mechanism of cyclodextrins modified composites was also proposed, and subsequent wastewater testing indicated that γCD-GO@Fe NPs had high potential for practical applications.
34823852	3	58	theme	@	457:457	arg1	NPs					473:475	γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	419:475	the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	408:475	The removal of OTC decreased in the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs, with better performance than that of bare GO and Fe NPs.
34823852	0	59	theme	@	54:54	arg1	composites					73:82	green synthesized graphene oxide@iron nanoparticle composites	22:82	green synthesized graphene oxide@iron nanoparticle composites for enhanced removal of oxytetracycline	22:122	Cyclodextrin modified green synthesized graphene oxide@iron nanoparticle composites for enhanced removal of oxytetracycline.
34823852	6	60	contain	had	877:879	arg1	modified					858:865	modified	858:865	modified	858:865	Cyclodextrin modified GO@Fe NPs had relatively small particle sizes (15 nm), with a high surface area (61.7 m2 · g-1).
34823852	6	60	contain	had	877:879	arg2	15 nm					914:918	15 nm	914:918	15 nm	914:918	Cyclodextrin modified GO@Fe NPs had relatively small particle sizes (15 nm), with a high surface area (61.7 m2 · g-1).
34823852	6	60	contain	had	877:879	arg2	sizes					907:911	relatively small particle sizes	881:911	relatively small particle sizes (15 nm)	881:919	Cyclodextrin modified GO@Fe NPs had relatively small particle sizes (15 nm), with a high surface area (61.7 m2 · g-1).
34823852	3	61	theme	@	469:469	arg1	NPs					473:475	γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	419:475	the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	408:475	The removal of OTC decreased in the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs, with better performance than that of bare GO and Fe NPs.
34823852	3	62	theme	Fe	458:459	arg1	NPs					473:475	γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	419:475	the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	408:475	The removal of OTC decreased in the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs, with better performance than that of bare GO and Fe NPs.
34823852	7	63	dep	agent	1109:1113	arg1	both					1078:1081	both	1078:1081	both	1078:1081	X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy suggested that cyclodextrin acted as both a stabilizing and capping agent during green synthesis, which could protect the reactivity of Fe NPs and simultaneously reduce aggregation.
34823852	8	64	theme	high	1378:1381	arg1	potential					1383:1391	high potential	1378:1391	high potential for practical applications	1378:1418	A potential synthesis mechanism of cyclodextrins modified composites was also proposed, and subsequent wastewater testing indicated that γCD-GO@Fe NPs had high potential for practical applications.
34823852	2	65	theme	iron	279:282	arg1	NPs					305:307	GO@Fe NPs	299:307	GO@Fe NPs	299:307	Here cyclodextrins (CDs) were successfully used to modify graphene-based iron nanoparticles (GO@Fe NPs) to enhance the absorption of oxytetracycline hydrochloride (OTC).
34823852	2	65	theme	iron	279:282	arg1	nanoparticles					284:296	graphene-based iron nanoparticles	264:296	graphene-based iron nanoparticles (GO@Fe NPs) to enhance the absorption of oxytetracycline hydrochloride (OTC)	264:373	Here cyclodextrins (CDs) were successfully used to modify graphene-based iron nanoparticles (GO@Fe NPs) to enhance the absorption of oxytetracycline hydrochloride (OTC).
34823852	3	66	theme	Fe	442:443	arg1	NPs					473:475	γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	419:475	the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	408:475	The removal of OTC decreased in the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs, with better performance than that of bare GO and Fe NPs.
34823852	4	67	theme	Characterization	535:550	arg1	techniques					552:561	Characterization techniques	535:561	Characterization techniques	535:561	Characterization techniques were applied to better understand how CDs impact the structure of GO@Fe NPs and improve removal performance.
34823852	7	68	theme	Fe	1177:1178	arg1	NPs					1180:1182	Fe NPs	1177:1182	Fe NPs	1177:1182	X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy suggested that cyclodextrin acted as both a stabilizing and capping agent during green synthesis, which could protect the reactivity of Fe NPs and simultaneously reduce aggregation.
34823852	8	69	theme	practical	1397:1405	arg1	applications					1407:1418	practical applications	1397:1418	practical applications	1397:1418	A potential synthesis mechanism of cyclodextrins modified composites was also proposed, and subsequent wastewater testing indicated that γCD-GO@Fe NPs had high potential for practical applications.
34823852	2	70	theme	graphene-based	264:277	arg1	NPs					305:307	GO@Fe NPs	299:307	GO@Fe NPs	299:307	Here cyclodextrins (CDs) were successfully used to modify graphene-based iron nanoparticles (GO@Fe NPs) to enhance the absorption of oxytetracycline hydrochloride (OTC).
34823852	2	70	theme	graphene-based	264:277	arg1	nanoparticles					284:296	graphene-based iron nanoparticles	264:296	graphene-based iron nanoparticles (GO@Fe NPs) to enhance the absorption of oxytetracycline hydrochloride (OTC)	264:373	Here cyclodextrins (CDs) were successfully used to modify graphene-based iron nanoparticles (GO@Fe NPs) to enhance the absorption of oxytetracycline hydrochloride (OTC).
34823852	1	71	theme	residual	141:148	arg1	antibiotics					150:160	residual antibiotics	141:160	residual antibiotics	141:160	The presence of residual antibiotics will lead to potential environmental risks.
34823852	8	72	theme	synthesis	1235:1243	arg1	mechanism					1245:1253	A potential synthesis mechanism	1223:1253	A potential synthesis mechanism of cyclodextrins modified composites	1223:1290	A potential synthesis mechanism of cyclodextrins modified composites was also proposed, and subsequent wastewater testing indicated that γCD-GO@Fe NPs had high potential for practical applications.
34823852	5	73	theme	removal	828:834	arg1	process					836:842	the removal process	824:842	the removal process	824:842	Raman and X-ray diffraction analysis showed that GO acted as a carrier to support Fe NPs within the grafted cyclodextrin, where GO also participated in the removal process.
34823852	8	74	theme	γCD-GO	1360:1365	arg1	NPs					1370:1372	γCD-GO@Fe NPs	1360:1372	γCD-GO@Fe NPs	1360:1372	A potential synthesis mechanism of cyclodextrins modified composites was also proposed, and subsequent wastewater testing indicated that γCD-GO@Fe NPs had high potential for practical applications.
34823852	6	75	theme	surface	934:940	arg1	61.7 m2 · g-1					948:960	61.7 m2 · g-1	948:960	61.7 m2 · g-1	948:960	Cyclodextrin modified GO@Fe NPs had relatively small particle sizes (15 nm), with a high surface area (61.7 m2 · g-1).
34823852	6	75	theme	surface	934:940	arg1	area					942:945	a high surface area	927:945	a high surface area (61.7 m2 · g-1)	927:961	Cyclodextrin modified GO@Fe NPs had relatively small particle sizes (15 nm), with a high surface area (61.7 m2 · g-1).
34823852	1	76	theme	antibiotics	150:160	arg1	presence					129:136	The presence	125:136	The presence of residual antibiotics	125:160	The presence of residual antibiotics will lead to potential environmental risks.
34823852	3	77	theme	γCD-GO	419:424	arg1	NPs					473:475	γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	419:475	the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs	408:475	The removal of OTC decreased in the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs, with better performance than that of bare GO and Fe NPs.
34823852	0	78	theme	nanoparticle	60:71	arg1	composites					73:82	green synthesized graphene oxide@iron nanoparticle composites	22:82	green synthesized graphene oxide@iron nanoparticle composites for enhanced removal of oxytetracycline	22:122	Cyclodextrin modified green synthesized graphene oxide@iron nanoparticle composites for enhanced removal of oxytetracycline.
34823852	3	79	theme	better	483:488	arg1	performance					490:500	better performance	483:500	better performance than that of bare GO and Fe NPs	483:532	The removal of OTC decreased in the order: γCD-GO@Fe NPs > βCD-GO@Fe NPs > αCD-GO@Fe NPs > GO@Fe NPs, with better performance than that of bare GO and Fe NPs.
34823852	7	80	theme	NPs	1180:1182	arg1	reactivity					1163:1172	the reactivity	1159:1172	the reactivity of Fe NPs	1159:1182	X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy suggested that cyclodextrin acted as both a stabilizing and capping agent during green synthesis, which could protect the reactivity of Fe NPs and simultaneously reduce aggregation.
33617497	9	0	theme	wastewater	1464:1473	arg1	treatment					1475:1483	wastewater treatment	1464:1483	wastewater treatment	1464:1483	The results introduced a novel support for PPO immobilization, and the immobilized PPO had great potential in wastewater treatment.
33617497	3	1	theme	×	510:510	arg1	103U/g					512:517	× 103U/g	510:517	× 103U/g for APPO and CPPO	510:535	Taguchi method was applied in the optimization of immobilization conditions resulting in the highest enzyme activity of 16.37 × 103 and 8.92 × 103U/g for APPO and CPPO, respectively.
33617497	0	2	theme	compounds	91:99	arg1	removal					101:107	phenolic compounds removal	82:107	phenolic compounds removal	82:107	Immobilization of polyphenol oxidase on chitosan/organic rectorite composites for phenolic compounds removal.
33617497	8	3	theme	immobilized	1177:1187	arg1	PPO					1189:1191	both immobilized PPO	1172:1191	both immobilized PPO	1172:1191	The order of removal percentage of phenolic compounds for both immobilized PPO was 2,4-DCP > 4-CP > PH. After 10 consecutive operations, the removal percentage of 2,4-DCP reached 73.2 ± 2.6% and 60.3 ± 1.5% for APPO and CPPO, respectively.
33617497	9	4	theme	great	1445:1449	arg1	potential					1451:1459	great potential	1445:1459	great potential	1445:1459	The results introduced a novel support for PPO immobilization, and the immobilized PPO had great potential in wastewater treatment.
33617497	3	5	theme	Taguchi	369:375	arg1	method					377:382	Taguchi method	369:382	Taguchi method	369:382	Taguchi method was applied in the optimization of immobilization conditions resulting in the highest enzyme activity of 16.37 × 103 and 8.92 × 103U/g for APPO and CPPO, respectively.
33617497	0	6	from	Immobilization	0:13	arg1	composites					67:76	chitosan/organic rectorite composites	40:76	chitosan/organic rectorite composites for phenolic compounds removal	40:107	Immobilization of polyphenol oxidase on chitosan/organic rectorite composites for phenolic compounds removal.
33617497	3	7	dep	×	495:495	arg1	103U/g					512:517	× 103U/g	510:517	× 103U/g for APPO and CPPO	510:535	Taguchi method was applied in the optimization of immobilization conditions resulting in the highest enzyme activity of 16.37 × 103 and 8.92 × 103U/g for APPO and CPPO, respectively.
33617497	5	8	theme	compound	710:717	arg1	percentage					687:696	The removal percentage	675:696	The removal percentage of phenolic compound, including phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP), by immobilized PPO	675:816	The removal percentage of phenolic compound, including phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP), by immobilized PPO was also explored.
33617497	7	9	theme	2,4-DCP	1027:1033	arg1	percentage					1000:1009	the removal percentage	988:1009	the removal percentage of PH, 4-CP and 2,4-DCP	988:1033	APPO contributed to a quick removal in the first hour, and the removal percentage of PH, 4-CP and 2,4-DCP could reach 69.3 ± 4.2%, 89.8 ± 2.5% and 93.8 ± 1.7% within 2 h, respectively.
33617497	2	10	theme	physical	313:320	arg1	APPO					334:337	APPO	334:337	APPO	334:337	Polyphenol oxidase (PPO) was immobilized on CTS/OREC by physical adsorption (APPO) and covalent binding (CPPO).
33617497	2	10	theme	physical	313:320	arg1	adsorption					322:331	physical adsorption	313:331	physical adsorption (APPO)	313:338	Polyphenol oxidase (PPO) was immobilized on CTS/OREC by physical adsorption (APPO) and covalent binding (CPPO).
33617497	5	11	theme	immobilized	802:812	arg1	PPO					814:816	immobilized PPO	802:816	immobilized PPO	802:816	The removal percentage of phenolic compound, including phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP), by immobilized PPO was also explored.
33617497	8	12	theme	4-CP	1207:1210	arg1	order					1118:1122	The order	1114:1122	The order of removal percentage of phenolic compounds for both immobilized PPO	1114:1191	The order of removal percentage of phenolic compounds for both immobilized PPO was 2,4-DCP > 4-CP > PH. After 10 consecutive operations, the removal percentage of 2,4-DCP reached 73.2 ± 2.6% and 60.3 ± 1.5% for APPO and CPPO, respectively.
33617497	8	12	theme	4-CP	1207:1210	arg1	>					1212:1212	2,4-DCP > 4-CP >	1197:1212	2,4-DCP > 4-CP >	1197:1212	The order of removal percentage of phenolic compounds for both immobilized PPO was 2,4-DCP > 4-CP > PH. After 10 consecutive operations, the removal percentage of 2,4-DCP reached 73.2 ± 2.6% and 60.3 ± 1.5% for APPO and CPPO, respectively.
33617497	4	13	theme	loading	639:645	arg1	capacity					647:654	the higher enzyme loading capacity	621:654	the higher enzyme loading capacity	621:654	APPO enzyme activity was higher than that of CPPO, while CPPO showed the higher enzyme loading capacity than that of APPO.
33617497	7	14	theme	±	1052:1052	arg1	%					1057:1057	69.3 ± 4.2%	1047:1057	69.3 ± 4.2%	1047:1057	APPO contributed to a quick removal in the first hour, and the removal percentage of PH, 4-CP and 2,4-DCP could reach 69.3 ± 4.2%, 89.8 ± 2.5% and 93.8 ± 1.7% within 2 h, respectively.
33617497	0	15	theme	chitosan/organic	40:55	arg1	composites					67:76	chitosan/organic rectorite composites	40:76	chitosan/organic rectorite composites for phenolic compounds removal	40:107	Immobilization of polyphenol oxidase on chitosan/organic rectorite composites for phenolic compounds removal.
33617497	8	16	theme	>	1205:1205	arg1	order					1118:1122	The order	1114:1122	The order of removal percentage of phenolic compounds for both immobilized PPO	1114:1191	The order of removal percentage of phenolic compounds for both immobilized PPO was 2,4-DCP > 4-CP > PH. After 10 consecutive operations, the removal percentage of 2,4-DCP reached 73.2 ± 2.6% and 60.3 ± 1.5% for APPO and CPPO, respectively.
33617497	8	16	theme	>	1205:1205	arg1	>					1212:1212	2,4-DCP > 4-CP >	1197:1212	2,4-DCP > 4-CP >	1197:1212	The order of removal percentage of phenolic compounds for both immobilized PPO was 2,4-DCP > 4-CP > PH. After 10 consecutive operations, the removal percentage of 2,4-DCP reached 73.2 ± 2.6% and 60.3 ± 1.5% for APPO and CPPO, respectively.
33617497	3	17	theme	highest	462:468	arg1	activity					477:484	the highest enzyme activity	458:484	the highest enzyme activity of 16.37 × 103 and 8.92 × 103U/g for APPO and CPPO, respectively	458:549	Taguchi method was applied in the optimization of immobilization conditions resulting in the highest enzyme activity of 16.37 × 103 and 8.92 × 103U/g for APPO and CPPO, respectively.
33617497	7	18	theme	±	1065:1065	arg1	%					1070:1070	89.8 ± 2.5%	1060:1070	89.8 ± 2.5%	1060:1070	APPO contributed to a quick removal in the first hour, and the removal percentage of PH, 4-CP and 2,4-DCP could reach 69.3 ± 4.2%, 89.8 ± 2.5% and 93.8 ± 1.7% within 2 h, respectively.
33617497	6	19	theme	compounds	900:908	arg1	removal					910:916	phenolic compounds removal	891:916	phenolic compounds removal	891:916	The results indicated that APPO was more efficient in phenolic compounds removal than CPPO.
33617497	4	20	theme	APPO	552:555	arg1	activity					564:571	APPO enzyme activity	552:571	APPO enzyme activity	552:571	APPO enzyme activity was higher than that of CPPO, while CPPO showed the higher enzyme loading capacity than that of APPO.
33617497	7	21	theme	PH	1014:1015	arg1	percentage					1000:1009	the removal percentage	988:1009	the removal percentage of PH, 4-CP and 2,4-DCP	988:1033	APPO contributed to a quick removal in the first hour, and the removal percentage of PH, 4-CP and 2,4-DCP could reach 69.3 ± 4.2%, 89.8 ± 2.5% and 93.8 ± 1.7% within 2 h, respectively.
33617497	9	22	contain	had	1441:1443	arg1	PPO					1437:1439	the immobilized PPO	1421:1439	the immobilized PPO	1421:1439	The results introduced a novel support for PPO immobilization, and the immobilized PPO had great potential in wastewater treatment.
33617497	9	22	contain	had	1441:1443	arg2	potential					1451:1459	great potential	1445:1459	great potential	1445:1459	The results introduced a novel support for PPO immobilization, and the immobilized PPO had great potential in wastewater treatment.
33617497	1	23	theme	Chitosan/organic	110:125	arg1	CTS/OREC					138:145	CTS/OREC	138:145	CTS/OREC	138:145	Chitosan/organic rectorite (CTS/OREC) composites were prepared and characterized by Fourier transform infrared spectrometry and X-ray diffraction.
33617497	1	23	theme	Chitosan/organic	110:125	arg1	rectorite					127:135	Chitosan/organic rectorite	110:135	Chitosan/organic rectorite (CTS/OREC) composites	110:157	Chitosan/organic rectorite (CTS/OREC) composites were prepared and characterized by Fourier transform infrared spectrometry and X-ray diffraction.
33617497	8	24	theme	removal	1255:1261	arg1	percentage					1263:1272	the removal percentage	1251:1272	the removal percentage of 2,4-DCP	1251:1283	The order of removal percentage of phenolic compounds for both immobilized PPO was 2,4-DCP > 4-CP > PH. After 10 consecutive operations, the removal percentage of 2,4-DCP reached 73.2 ± 2.6% and 60.3 ± 1.5% for APPO and CPPO, respectively.
33617497	0	25	theme	oxidase	29:35	arg1	Immobilization					0:13	Immobilization	0:13	Immobilization of polyphenol oxidase on chitosan/organic rectorite composites for phenolic compounds removal.	0:108	Immobilization of polyphenol oxidase on chitosan/organic rectorite composites for phenolic compounds removal.
33617497	6	26	from	removal	910:916	arg1	efficient					878:886	efficient	878:886	efficient	878:886	The results indicated that APPO was more efficient in phenolic compounds removal than CPPO.
33617497	2	27	theme	Polyphenol	257:266	arg1	PPO					277:279	PPO	277:279	PPO	277:279	Polyphenol oxidase (PPO) was immobilized on CTS/OREC by physical adsorption (APPO) and covalent binding (CPPO).
33617497	2	27	theme	Polyphenol	257:266	arg1	oxidase					268:274	Polyphenol oxidase	257:274	Polyphenol oxidase (PPO)	257:280	Polyphenol oxidase (PPO) was immobilized on CTS/OREC by physical adsorption (APPO) and covalent binding (CPPO).
33617497	1	28	theme	rectorite	127:135	arg1	composites					148:157	Chitosan/organic rectorite (CTS/OREC) composites	110:157	Chitosan/organic rectorite (CTS/OREC) composites	110:157	Chitosan/organic rectorite (CTS/OREC) composites were prepared and characterized by Fourier transform infrared spectrometry and X-ray diffraction.
33617497	7	29	theme	4-CP	1018:1021	arg1	percentage					1000:1009	the removal percentage	988:1009	the removal percentage of PH, 4-CP and 2,4-DCP	988:1033	APPO contributed to a quick removal in the first hour, and the removal percentage of PH, 4-CP and 2,4-DCP could reach 69.3 ± 4.2%, 89.8 ± 2.5% and 93.8 ± 1.7% within 2 h, respectively.
33617497	0	30	theme	polyphenol	18:27	arg1	oxidase					29:35	polyphenol oxidase	18:35	polyphenol oxidase	18:35	Immobilization of polyphenol oxidase on chitosan/organic rectorite composites for phenolic compounds removal.
33617497	5	31	theme	removal	679:685	arg1	percentage					687:696	The removal percentage	675:696	The removal percentage of phenolic compound, including phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP), by immobilized PPO	675:816	The removal percentage of phenolic compound, including phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP), by immobilized PPO was also explored.
33617497	9	32	theme	novel	1379:1383	arg1	support					1385:1391	a novel support	1377:1391	a novel support for PPO immobilization	1377:1414	The results introduced a novel support for PPO immobilization, and the immobilized PPO had great potential in wastewater treatment.
33617497	1	33	theme	X-ray	238:242	arg1	diffraction					244:254	X-ray diffraction	238:254	X-ray diffraction	238:254	Chitosan/organic rectorite (CTS/OREC) composites were prepared and characterized by Fourier transform infrared spectrometry and X-ray diffraction.
33617497	3	34	theme	immobilization	419:432	arg1	conditions					434:443	immobilization conditions	419:443	immobilization conditions resulting in the highest enzyme activity of 16.37 × 103 and 8.92 × 103U/g for APPO and CPPO, respectively	419:549	Taguchi method was applied in the optimization of immobilization conditions resulting in the highest enzyme activity of 16.37 × 103 and 8.92 × 103U/g for APPO and CPPO, respectively.
33617497	4	35	theme	enzyme	557:562	arg1	activity					564:571	APPO enzyme activity	552:571	APPO enzyme activity	552:571	APPO enzyme activity was higher than that of CPPO, while CPPO showed the higher enzyme loading capacity than that of APPO.
33617497	4	36	theme	higher	625:630	arg1	capacity					647:654	the higher enzyme loading capacity	621:654	the higher enzyme loading capacity	621:654	APPO enzyme activity was higher than that of CPPO, while CPPO showed the higher enzyme loading capacity than that of APPO.
33617497	9	37	theme	PPO	1397:1399	arg1	immobilization					1401:1414	PPO immobilization	1397:1414	PPO immobilization	1397:1414	The results introduced a novel support for PPO immobilization, and the immobilized PPO had great potential in wastewater treatment.
33617497	3	38	theme	enzyme	470:475	arg1	activity					477:484	the highest enzyme activity	458:484	the highest enzyme activity of 16.37 × 103 and 8.92 × 103U/g for APPO and CPPO, respectively	458:549	Taguchi method was applied in the optimization of immobilization conditions resulting in the highest enzyme activity of 16.37 × 103 and 8.92 × 103U/g for APPO and CPPO, respectively.
33617497	3	39	theme	conditions	434:443	arg1	optimization					403:414	the optimization	399:414	the optimization of immobilization conditions resulting in the highest enzyme activity of 16.37 × 103 and 8.92 × 103U/g for APPO and CPPO, respectively	399:549	Taguchi method was applied in the optimization of immobilization conditions resulting in the highest enzyme activity of 16.37 × 103 and 8.92 × 103U/g for APPO and CPPO, respectively.
33617497	6	40	theme	phenolic	891:898	arg1	compounds					900:908	phenolic compounds	891:908	phenolic compounds removal	891:916	The results indicated that APPO was more efficient in phenolic compounds removal than CPPO.
33617497	9	41	theme	immobilized	1425:1435	arg1	PPO					1437:1439	the immobilized PPO	1421:1439	the immobilized PPO	1421:1439	The results introduced a novel support for PPO immobilization, and the immobilized PPO had great potential in wastewater treatment.
33617497	8	42	theme	removal	1127:1133	arg1	percentage					1135:1144	removal percentage	1127:1144	removal percentage of phenolic compounds	1127:1166	The order of removal percentage of phenolic compounds for both immobilized PPO was 2,4-DCP > 4-CP > PH. After 10 consecutive operations, the removal percentage of 2,4-DCP reached 73.2 ± 2.6% and 60.3 ± 1.5% for APPO and CPPO, respectively.
33617497	8	43	theme	consecutive	1227:1237	arg1	operations					1239:1248	10 consecutive operations	1224:1248	10 consecutive operations	1224:1248	The order of removal percentage of phenolic compounds for both immobilized PPO was 2,4-DCP > 4-CP > PH. After 10 consecutive operations, the removal percentage of 2,4-DCP reached 73.2 ± 2.6% and 60.3 ± 1.5% for APPO and CPPO, respectively.
33617497	8	44	theme	phenolic	1149:1156	arg1	compounds					1158:1166	phenolic compounds	1149:1166	phenolic compounds	1149:1166	The order of removal percentage of phenolic compounds for both immobilized PPO was 2,4-DCP > 4-CP > PH. After 10 consecutive operations, the removal percentage of 2,4-DCP reached 73.2 ± 2.6% and 60.3 ± 1.5% for APPO and CPPO, respectively.
33617497	5	45	theme	phenolic	701:708	arg1	compound					710:717	phenolic compound	701:717	phenolic compound	701:717	The removal percentage of phenolic compound, including phenol (PH), 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP), by immobilized PPO was also explored.
33617497	6	46	from	efficient	878:886	arg1	removal					910:916	phenolic compounds removal	891:916	phenolic compounds removal	891:916	The results indicated that APPO was more efficient in phenolic compounds removal than CPPO.
33617497	8	47	theme	±	1298:1298	arg1	%					1303:1303	73.2 ± 2.6%	1293:1303	73.2 ± 2.6%	1293:1303	The order of removal percentage of phenolic compounds for both immobilized PPO was 2,4-DCP > 4-CP > PH. After 10 consecutive operations, the removal percentage of 2,4-DCP reached 73.2 ± 2.6% and 60.3 ± 1.5% for APPO and CPPO, respectively.
33617497	1	48	dep	Fourier	194:200	arg1	transform					202:210	transform	202:210	transform infrared spectrometry and X-ray diffraction	202:254	Chitosan/organic rectorite (CTS/OREC) composites were prepared and characterized by Fourier transform infrared spectrometry and X-ray diffraction.
33617497	0	49	theme	rectorite	57:65	arg1	composites					67:76	chitosan/organic rectorite composites	40:76	chitosan/organic rectorite composites for phenolic compounds removal	40:107	Immobilization of polyphenol oxidase on chitosan/organic rectorite composites for phenolic compounds removal.
33617497	7	50	theme	removal	992:998	arg1	percentage					1000:1009	the removal percentage	988:1009	the removal percentage of PH, 4-CP and 2,4-DCP	988:1033	APPO contributed to a quick removal in the first hour, and the removal percentage of PH, 4-CP and 2,4-DCP could reach 69.3 ± 4.2%, 89.8 ± 2.5% and 93.8 ± 1.7% within 2 h, respectively.
33617497	1	51	dep	transform	202:210	arg1	infrared					212:219	infrared	212:219	transform infrared spectrometry and X-ray diffraction	202:254	Chitosan/organic rectorite (CTS/OREC) composites were prepared and characterized by Fourier transform infrared spectrometry and X-ray diffraction.
33617497	7	52	from	removal	957:963	arg1	hour					978:981	the first hour	968:981	the first hour	968:981	APPO contributed to a quick removal in the first hour, and the removal percentage of PH, 4-CP and 2,4-DCP could reach 69.3 ± 4.2%, 89.8 ± 2.5% and 93.8 ± 1.7% within 2 h, respectively.
33617497	8	53	theme	percentage	1135:1144	arg1	order					1118:1122	The order	1114:1122	The order of removal percentage of phenolic compounds for both immobilized PPO	1114:1191	The order of removal percentage of phenolic compounds for both immobilized PPO was 2,4-DCP > 4-CP > PH. After 10 consecutive operations, the removal percentage of 2,4-DCP reached 73.2 ± 2.6% and 60.3 ± 1.5% for APPO and CPPO, respectively.
33617497	8	53	theme	percentage	1135:1144	arg1	>					1212:1212	2,4-DCP > 4-CP >	1197:1212	2,4-DCP > 4-CP >	1197:1212	The order of removal percentage of phenolic compounds for both immobilized PPO was 2,4-DCP > 4-CP > PH. After 10 consecutive operations, the removal percentage of 2,4-DCP reached 73.2 ± 2.6% and 60.3 ± 1.5% for APPO and CPPO, respectively.
33617497	7	54	theme	quick	951:955	arg1	removal					957:963	a quick removal	949:963	a quick removal in the first hour	949:981	APPO contributed to a quick removal in the first hour, and the removal percentage of PH, 4-CP and 2,4-DCP could reach 69.3 ± 4.2%, 89.8 ± 2.5% and 93.8 ± 1.7% within 2 h, respectively.
33617497	3	55	theme	×	495:495	arg1	activity					477:484	the highest enzyme activity	458:484	the highest enzyme activity of 16.37 × 103 and 8.92 × 103U/g for APPO and CPPO, respectively	458:549	Taguchi method was applied in the optimization of immobilization conditions resulting in the highest enzyme activity of 16.37 × 103 and 8.92 × 103U/g for APPO and CPPO, respectively.
33617497	7	56	theme	first	972:976	arg1	hour					978:981	the first hour	968:981	the first hour	968:981	APPO contributed to a quick removal in the first hour, and the removal percentage of PH, 4-CP and 2,4-DCP could reach 69.3 ± 4.2%, 89.8 ± 2.5% and 93.8 ± 1.7% within 2 h, respectively.
33617497	8	57	theme	±	1314:1314	arg1	%					1319:1319	60.3 ± 1.5%	1309:1319	60.3 ± 1.5% for APPO and CPPO	1309:1337	The order of removal percentage of phenolic compounds for both immobilized PPO was 2,4-DCP > 4-CP > PH. After 10 consecutive operations, the removal percentage of 2,4-DCP reached 73.2 ± 2.6% and 60.3 ± 1.5% for APPO and CPPO, respectively.
33617497	8	58	theme	2,4-DCP	1197:1203	arg1	order					1118:1122	The order	1114:1122	The order of removal percentage of phenolic compounds for both immobilized PPO	1114:1191	The order of removal percentage of phenolic compounds for both immobilized PPO was 2,4-DCP > 4-CP > PH. After 10 consecutive operations, the removal percentage of 2,4-DCP reached 73.2 ± 2.6% and 60.3 ± 1.5% for APPO and CPPO, respectively.
33617497	8	58	theme	2,4-DCP	1197:1203	arg1	>					1212:1212	2,4-DCP > 4-CP >	1197:1212	2,4-DCP > 4-CP >	1197:1212	The order of removal percentage of phenolic compounds for both immobilized PPO was 2,4-DCP > 4-CP > PH. After 10 consecutive operations, the removal percentage of 2,4-DCP reached 73.2 ± 2.6% and 60.3 ± 1.5% for APPO and CPPO, respectively.
33617497	2	59	theme	covalent	344:351	arg1	CPPO					362:365	CPPO	362:365	CPPO	362:365	Polyphenol oxidase (PPO) was immobilized on CTS/OREC by physical adsorption (APPO) and covalent binding (CPPO).
33617497	2	59	theme	covalent	344:351	arg1	binding					353:359	covalent binding	344:359	covalent binding (CPPO)	344:366	Polyphenol oxidase (PPO) was immobilized on CTS/OREC by physical adsorption (APPO) and covalent binding (CPPO).
33617497	8	60	theme	compounds	1158:1166	arg1	percentage					1135:1144	removal percentage	1127:1144	removal percentage of phenolic compounds	1127:1166	The order of removal percentage of phenolic compounds for both immobilized PPO was 2,4-DCP > 4-CP > PH. After 10 consecutive operations, the removal percentage of 2,4-DCP reached 73.2 ± 2.6% and 60.3 ± 1.5% for APPO and CPPO, respectively.
33617497	0	61	theme	phenolic	82:89	arg1	compounds					91:99	phenolic compounds	82:99	phenolic compounds removal	82:107	Immobilization of polyphenol oxidase on chitosan/organic rectorite composites for phenolic compounds removal.
33617497	8	62	theme	2,4-DCP	1277:1283	arg1	percentage					1263:1272	the removal percentage	1251:1272	the removal percentage of 2,4-DCP	1251:1283	The order of removal percentage of phenolic compounds for both immobilized PPO was 2,4-DCP > 4-CP > PH. After 10 consecutive operations, the removal percentage of 2,4-DCP reached 73.2 ± 2.6% and 60.3 ± 1.5% for APPO and CPPO, respectively.
33617497	4	63	theme	enzyme	632:637	arg1	capacity					647:654	the higher enzyme loading capacity	621:654	the higher enzyme loading capacity	621:654	APPO enzyme activity was higher than that of CPPO, while CPPO showed the higher enzyme loading capacity than that of APPO.
33617497	7	64	theme	±	1081:1081	arg1	%					1086:1086	93.8 ± 1.7%	1076:1086	93.8 ± 1.7% within 2 h	1076:1097	APPO contributed to a quick removal in the first hour, and the removal percentage of PH, 4-CP and 2,4-DCP could reach 69.3 ± 4.2%, 89.8 ± 2.5% and 93.8 ± 1.7% within 2 h, respectively.
33278967	5	0	theme	inhibitory	920:929	arg1	activities					931:940	higher α-amylase and α-glucosidase inhibitory activities	885:940	higher α-amylase and α-glucosidase inhibitory activities	885:940	WSRP-2a promoted more proliferation, NO release, and the secretion of cytokines in RAW264.7 macrophages, while WSRP-2b presented higher α-amylase and α-glucosidase inhibitory activities.
33278967	5	1	theme	NO	793:794	arg1	release					796:802	NO release	793:802	NO release	793:802	WSRP-2a promoted more proliferation, NO release, and the secretion of cytokines in RAW264.7 macrophages, while WSRP-2b presented higher α-amylase and α-glucosidase inhibitory activities.
33278967	5	2	dep	α-amylase	892:900	arg1	higher					885:890	higher	885:890	higher	885:890	WSRP-2a promoted more proliferation, NO release, and the secretion of cytokines in RAW264.7 macrophages, while WSRP-2b presented higher α-amylase and α-glucosidase inhibitory activities.
33278967	1	3	theme	polysaccharide	155:168	arg1	components					170:179	two polysaccharide components	151:179	two polysaccharide components	151:179	In this study, two polysaccharide components named WSRP-2a and WSRP-2b, were purified from Rosa setate x Rosa rugosa waste via anion exchange and gel filtration chromatography.
33278967	1	4	theme	rugosa	246:251	arg1	waste					253:257	rugosa waste	246:257	rugosa waste	246:257	In this study, two polysaccharide components named WSRP-2a and WSRP-2b, were purified from Rosa setate x Rosa rugosa waste via anion exchange and gel filtration chromatography.
33278967	0	5	theme	x	115:115	arg1	waste					129:133	x Rosa rugosa waste	115:133	x Rosa rugosa waste	115:133	Structure characteristics, hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate x Rosa rugosa waste.
33278967	3	6	theme	kD	645:646	arg1	weight					630:635	molecular weight	620:635	molecular weight of 56.8 kD and 23.9 kD	620:658	WSRP-2a and WSRP-2b, however, differed in molecular weight of 56.8 kD and 23.9 kD.
33278967	5	7	theme	α-glucosidase	906:918	arg1	activities					931:940	higher α-amylase and α-glucosidase inhibitory activities	885:940	higher α-amylase and α-glucosidase inhibitory activities	885:940	WSRP-2a promoted more proliferation, NO release, and the secretion of cytokines in RAW264.7 macrophages, while WSRP-2b presented higher α-amylase and α-glucosidase inhibitory activities.
33278967	6	8	theme	waste	1016:1020	arg1	biomass					1022:1028	the Rosa Setate x Rosa Rugosa waste biomass	986:1028	the Rosa Setate x Rosa Rugosa waste biomass	986:1028	Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
33278967	6	8	theme	waste	1016:1020	arg1	source					1059:1064	a promising source	1047:1064	a promising source of bioactive pectic polysaccharides	1047:1100	Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
33278967	6	9	theme	Rugosa	1009:1014	arg1	biomass					1022:1028	the Rosa Setate x Rosa Rugosa waste biomass	986:1028	the Rosa Setate x Rosa Rugosa waste biomass	986:1028	Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
33278967	6	9	theme	Rugosa	1009:1014	arg1	source					1059:1064	a promising source	1047:1064	a promising source of bioactive pectic polysaccharides	1047:1100	Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
33278967	0	10	theme	rugosa	122:127	arg1	waste					129:133	x Rosa rugosa waste	115:133	x Rosa rugosa waste	115:133	Structure characteristics, hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate x Rosa rugosa waste.
33278967	0	11	from	activities	61:70	arg1	Rosa					103:106	Rosa	103:106	Rosa	103:106	Structure characteristics, hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate x Rosa rugosa waste.
33278967	6	12	theme	polysaccharides	1086:1100	arg1	biomass					1022:1028	the Rosa Setate x Rosa Rugosa waste biomass	986:1028	the Rosa Setate x Rosa Rugosa waste biomass	986:1028	Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
33278967	6	12	theme	polysaccharides	1086:1100	arg1	source					1059:1064	a promising source	1047:1064	a promising source of bioactive pectic polysaccharides	1047:1100	Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
33278967	0	13	theme	Rosa	117:120	arg1	waste					129:133	x Rosa rugosa waste	115:133	x Rosa rugosa waste	115:133	Structure characteristics, hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate x Rosa rugosa waste.
33278967	6	14	used	used	1039:1042	arg2	source					1059:1064	a promising source	1047:1064	a promising source of bioactive pectic polysaccharides	1047:1100	Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
33278967	6	14	used	used	1039:1042	arg2	biomass					1022:1028	the Rosa Setate x Rosa Rugosa waste biomass	986:1028	the Rosa Setate x Rosa Rugosa waste biomass	986:1028	Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
33278967	6	15	theme	Rosa	990:993	arg1	biomass					1022:1028	the Rosa Setate x Rosa Rugosa waste biomass	986:1028	the Rosa Setate x Rosa Rugosa waste biomass	986:1028	Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
33278967	6	15	theme	Rosa	990:993	arg1	source					1059:1064	a promising source	1047:1064	a promising source of bioactive pectic polysaccharides	1047:1100	Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
33278967	5	16	theme	more	773:776	arg1	proliferation					778:790	more proliferation	773:790	more proliferation	773:790	WSRP-2a promoted more proliferation, NO release, and the secretion of cytokines in RAW264.7 macrophages, while WSRP-2b presented higher α-amylase and α-glucosidase inhibitory activities.
33278967	2	17	theme	Monosaccharide	313:326	arg1	composition					328:338	Monosaccharide composition	313:338	Monosaccharide composition	313:338	Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis confirmed that both of these fractions were mainly composed of galacturonic acid, arabinose, galactose and rhamnose, which were pectin-type polysaccharides with non-triple-helix structure.
33278967	2	18	theme	spectra	372:378	arg1	analysis					380:387	Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis	313:387	Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis	313:387	Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis confirmed that both of these fractions were mainly composed of galacturonic acid, arabinose, galactose and rhamnose, which were pectin-type polysaccharides with non-triple-helix structure.
33278967	0	19	theme	Structure	0:8	arg1	activities					61:70	hypoglycemic and immunomodulatory activities	27:70	hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa	27:106	Structure characteristics, hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate x Rosa rugosa waste.
33278967	0	19	theme	Structure	0:8	arg1	characteristics					10:24	Structure characteristics	0:24	Structure characteristics	0:24	Structure characteristics, hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate x Rosa rugosa waste.
33278967	1	20	theme	anion	263:267	arg1	exchange					269:276	anion exchange	263:276	anion exchange	263:276	In this study, two polysaccharide components named WSRP-2a and WSRP-2b, were purified from Rosa setate x Rosa rugosa waste via anion exchange and gel filtration chromatography.
33278967	2	21	theme	NMR	368:370	arg1	spectra					372:378	NMR spectra	368:378	NMR spectra	368:378	Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis confirmed that both of these fractions were mainly composed of galacturonic acid, arabinose, galactose and rhamnose, which were pectin-type polysaccharides with non-triple-helix structure.
33278967	0	22	theme	hypoglycemic	27:38	arg1	activities					61:70	hypoglycemic and immunomodulatory activities	27:70	hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa	27:106	Structure characteristics, hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate x Rosa rugosa waste.
33278967	0	22	theme	hypoglycemic	27:38	arg1	characteristics					10:24	Structure characteristics	0:24	Structure characteristics	0:24	Structure characteristics, hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate x Rosa rugosa waste.
33278967	6	23	theme	x	1002:1002	arg1	biomass					1022:1028	the Rosa Setate x Rosa Rugosa waste biomass	986:1028	the Rosa Setate x Rosa Rugosa waste biomass	986:1028	Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
33278967	6	23	theme	x	1002:1002	arg1	source					1059:1064	a promising source	1047:1064	a promising source of bioactive pectic polysaccharides	1047:1100	Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
33278967	2	24	theme	non-triple-helix	550:565	arg1	structure					567:575	non-triple-helix structure	550:575	non-triple-helix structure	550:575	Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis confirmed that both of these fractions were mainly composed of galacturonic acid, arabinose, galactose and rhamnose, which were pectin-type polysaccharides with non-triple-helix structure.
33278967	1	25	dep	setate	232:237	arg1	waste					253:257	rugosa waste	246:257	rugosa waste	246:257	In this study, two polysaccharide components named WSRP-2a and WSRP-2b, were purified from Rosa setate x Rosa rugosa waste via anion exchange and gel filtration chromatography.
33278967	5	26	theme	RAW264.7	839:846	arg1	macrophages					848:858	RAW264.7 macrophages	839:858	RAW264.7 macrophages	839:858	WSRP-2a promoted more proliferation, NO release, and the secretion of cytokines in RAW264.7 macrophages, while WSRP-2b presented higher α-amylase and α-glucosidase inhibitory activities.
33278967	6	27	theme	Setate	995:1000	arg1	biomass					1022:1028	the Rosa Setate x Rosa Rugosa waste biomass	986:1028	the Rosa Setate x Rosa Rugosa waste biomass	986:1028	Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
33278967	6	27	theme	Setate	995:1000	arg1	source					1059:1064	a promising source	1047:1064	a promising source of bioactive pectic polysaccharides	1047:1100	Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
33278967	0	28	from	Rosa	103:106	arg1	activities					61:70	hypoglycemic and immunomodulatory activities	27:70	hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa	27:106	Structure characteristics, hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate x Rosa rugosa waste.
33278967	0	28	from	Rosa	103:106	arg1	polysaccharides					82:96	pectic polysaccharides	75:96	pectic polysaccharides from Rosa	75:106	Structure characteristics, hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate x Rosa rugosa waste.
33278967	0	28	from	Rosa	103:106	arg1	characteristics					10:24	Structure characteristics	0:24	Structure characteristics	0:24	Structure characteristics, hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate x Rosa rugosa waste.
33278967	2	29	theme	assay	351:355	arg1	analysis					380:387	Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis	313:387	Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis	313:387	Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis confirmed that both of these fractions were mainly composed of galacturonic acid, arabinose, galactose and rhamnose, which were pectin-type polysaccharides with non-triple-helix structure.
33278967	0	30	theme	immunomodulatory	44:59	arg1	activities					61:70	hypoglycemic and immunomodulatory activities	27:70	hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa	27:106	Structure characteristics, hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate x Rosa rugosa waste.
33278967	0	30	theme	immunomodulatory	44:59	arg1	characteristics					10:24	Structure characteristics	0:24	Structure characteristics	0:24	Structure characteristics, hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate x Rosa rugosa waste.
33278967	1	31	theme	gel	282:284	arg1	chromatography					297:310	gel filtration chromatography	282:310	gel filtration chromatography	282:310	In this study, two polysaccharide components named WSRP-2a and WSRP-2b, were purified from Rosa setate x Rosa rugosa waste via anion exchange and gel filtration chromatography.
33278967	5	32	theme	α-amylase	892:900	arg1	activities					931:940	higher α-amylase and α-glucosidase inhibitory activities	885:940	higher α-amylase and α-glucosidase inhibitory activities	885:940	WSRP-2a promoted more proliferation, NO release, and the secretion of cytokines in RAW264.7 macrophages, while WSRP-2b presented higher α-amylase and α-glucosidase inhibitory activities.
33278967	1	33	theme	filtration	286:295	arg1	chromatography					297:310	gel filtration chromatography	282:310	gel filtration chromatography	282:310	In this study, two polysaccharide components named WSRP-2a and WSRP-2b, were purified from Rosa setate x Rosa rugosa waste via anion exchange and gel filtration chromatography.
33278967	2	34	theme	pectin-type	517:527	arg1	polysaccharides					529:543	pectin-type polysaccharides	517:543	pectin-type polysaccharides with non-triple-helix structure	517:575	Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis confirmed that both of these fractions were mainly composed of galacturonic acid, arabinose, galactose and rhamnose, which were pectin-type polysaccharides with non-triple-helix structure.
33278967	5	35	theme	cytokines	826:834	arg1	proliferation					778:790	more proliferation	773:790	more proliferation	773:790	WSRP-2a promoted more proliferation, NO release, and the secretion of cytokines in RAW264.7 macrophages, while WSRP-2b presented higher α-amylase and α-glucosidase inhibitory activities.
33278967	5	35	theme	cytokines	826:834	arg1	release					796:802	NO release	793:802	NO release	793:802	WSRP-2a promoted more proliferation, NO release, and the secretion of cytokines in RAW264.7 macrophages, while WSRP-2b presented higher α-amylase and α-glucosidase inhibitory activities.
33278967	5	35	theme	cytokines	826:834	arg1	secretion					813:821	the secretion	809:821	the secretion of cytokines	809:834	WSRP-2a promoted more proliferation, NO release, and the secretion of cytokines in RAW264.7 macrophages, while WSRP-2b presented higher α-amylase and α-glucosidase inhibitory activities.
33278967	6	36	theme	promising	1049:1057	arg1	biomass					1022:1028	the Rosa Setate x Rosa Rugosa waste biomass	986:1028	the Rosa Setate x Rosa Rugosa waste biomass	986:1028	Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
33278967	6	36	theme	promising	1049:1057	arg1	source					1059:1064	a promising source	1047:1064	a promising source of bioactive pectic polysaccharides	1047:1100	Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
33278967	2	37	theme	galacturonic	452:463	arg1	acid					465:468	galacturonic acid	452:468	galacturonic acid	452:468	Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis confirmed that both of these fractions were mainly composed of galacturonic acid, arabinose, galactose and rhamnose, which were pectin-type polysaccharides with non-triple-helix structure.
33278967	2	38	theme	red	347:349	arg1	assay					351:355	Congo red assay	341:355	Congo red assay	341:355	Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis confirmed that both of these fractions were mainly composed of galacturonic acid, arabinose, galactose and rhamnose, which were pectin-type polysaccharides with non-triple-helix structure.
33278967	4	39	theme	impressive	699:708	arg1	activities					744:753	their impressive hypoglycemic and immunomodulatory activities	693:753	their impressive hypoglycemic and immunomodulatory activities	693:753	The followed bioassay presented their impressive hypoglycemic and immunomodulatory activities.
33278967	2	40	with	polysaccharides	529:543	arg1	structure					567:575	non-triple-helix structure	550:575	non-triple-helix structure	550:575	Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis confirmed that both of these fractions were mainly composed of galacturonic acid, arabinose, galactose and rhamnose, which were pectin-type polysaccharides with non-triple-helix structure.
33278967	4	41	theme	hypoglycemic	710:721	arg1	activities					744:753	their impressive hypoglycemic and immunomodulatory activities	693:753	their impressive hypoglycemic and immunomodulatory activities	693:753	The followed bioassay presented their impressive hypoglycemic and immunomodulatory activities.
33278967	3	42	theme	molecular	620:628	arg1	weight					630:635	molecular weight	620:635	molecular weight of 56.8 kD and 23.9 kD	620:658	WSRP-2a and WSRP-2b, however, differed in molecular weight of 56.8 kD and 23.9 kD.
33278967	4	43	theme	followed	665:672	arg1	bioassay					674:681	The followed bioassay	661:681	The followed bioassay	661:681	The followed bioassay presented their impressive hypoglycemic and immunomodulatory activities.
33278967	4	44	theme	immunomodulatory	727:742	arg1	activities					744:753	their impressive hypoglycemic and immunomodulatory activities	693:753	their impressive hypoglycemic and immunomodulatory activities	693:753	The followed bioassay presented their impressive hypoglycemic and immunomodulatory activities.
33278967	6	45	theme	pectic	1079:1084	arg1	polysaccharides					1086:1100	bioactive pectic polysaccharides	1069:1100	bioactive pectic polysaccharides	1069:1100	Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
33278967	2	46	theme	composition	328:338	arg1	analysis					380:387	Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis	313:387	Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis	313:387	Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis confirmed that both of these fractions were mainly composed of galacturonic acid, arabinose, galactose and rhamnose, which were pectin-type polysaccharides with non-triple-helix structure.
33278967	0	47	theme	polysaccharides	82:96	arg1	activities					61:70	hypoglycemic and immunomodulatory activities	27:70	hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa	27:106	Structure characteristics, hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate x Rosa rugosa waste.
33278967	0	47	theme	polysaccharides	82:96	arg1	characteristics					10:24	Structure characteristics	0:24	Structure characteristics	0:24	Structure characteristics, hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate x Rosa rugosa waste.
33278967	3	48	theme	kD	657:658	arg1	weight					630:635	molecular weight	620:635	molecular weight of 56.8 kD and 23.9 kD	620:658	WSRP-2a and WSRP-2b, however, differed in molecular weight of 56.8 kD and 23.9 kD.
33278967	6	49	theme	bioactive	1069:1077	arg1	polysaccharides					1086:1100	bioactive pectic polysaccharides	1069:1100	bioactive pectic polysaccharides	1069:1100	Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
33278967	0	50	theme	pectic	75:80	arg1	polysaccharides					82:96	pectic polysaccharides	75:96	pectic polysaccharides from Rosa	75:106	Structure characteristics, hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate x Rosa rugosa waste.
33278967	6	51	theme	Rosa	1004:1007	arg1	biomass					1022:1028	the Rosa Setate x Rosa Rugosa waste biomass	986:1028	the Rosa Setate x Rosa Rugosa waste biomass	986:1028	Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
33278967	6	51	theme	Rosa	1004:1007	arg1	source					1059:1064	a promising source	1047:1064	a promising source of bioactive pectic polysaccharides	1047:1100	Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides.
33278967	2	52	theme	FT-IR	358:362	arg1	analysis					380:387	Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis	313:387	Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis	313:387	Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis confirmed that both of these fractions were mainly composed of galacturonic acid, arabinose, galactose and rhamnose, which were pectin-type polysaccharides with non-triple-helix structure.
34053549	8	0	theme	parameters	1057:1066	arg1	perceptions					1072:1082	perceptions	1072:1082	perceptions	1072:1082	Using sensory panelists, oral processing of emulsion gels was examined in terms of both mastication parameters and perceptions of oiliness and thickness.
34053549	8	0	theme	parameters	1057:1066	arg1	terms					1031:1035	terms	1031:1035	terms of both mastication parameters	1031:1066	Using sensory panelists, oral processing of emulsion gels was examined in terms of both mastication parameters and perceptions of oiliness and thickness.
34053549	9	1	theme	oil	1190:1192	arg1	release					1194:1200	a higher oil release	1181:1200	a higher oil release	1181:1200	The obtained results showed that the use of a starch emulsifier gives a higher oil release and an enhanced oral sensation of oiliness mouthfeel.
34053549	10	2	theme	products	1348:1355	arg1	design					1321:1326	the design	1317:1326	the design of fat-reduced food products	1317:1355	Therefore, starch emulsion could provide a novel solution in the design of fat-reduced food products with no effect on the mastication parameter, sensation and perception of fat-related attributes.
34053549	4	3	used	used	587:590	arg2	emulsifier					572:581	whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier	518:581	whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier	518:581	On the other hand, whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier was used as a reference.
34053549	4	3	used	used	587:590	arg2	reference					597:605	a reference	595:605	a reference	595:605	On the other hand, whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier was used as a reference.
34053549	0	4	theme	gels	70:73	arg1	release					18:24	oral oil release	9:24	oral oil release	9:24	Enhanced oral oil release and mouthfeel perception of starch emulsion gels.
34053549	0	4	theme	gels	70:73	arg1	perception					40:49	mouthfeel perception	30:49	mouthfeel perception	30:49	Enhanced oral oil release and mouthfeel perception of starch emulsion gels.
34053549	10	5	with	solution	1305:1312	arg1	effect					1365:1370	no effect	1362:1370	no effect on the mastication parameter, sensation and perception of fat-related attributes	1362:1451	Therefore, starch emulsion could provide a novel solution in the design of fat-reduced food products with no effect on the mastication parameter, sensation and perception of fat-related attributes.
34053549	10	6	theme	food	1343:1346	arg1	products					1348:1355	fat-reduced food products	1331:1355	fat-reduced food products	1331:1355	Therefore, starch emulsion could provide a novel solution in the design of fat-reduced food products with no effect on the mastication parameter, sensation and perception of fat-related attributes.
34053549	10	7	theme	novel	1299:1303	arg1	solution					1305:1312	a novel solution	1297:1312	a novel solution in the design of fat-reduced food products with no effect on the mastication parameter, sensation and perception of fat-related attributes	1297:1451	Therefore, starch emulsion could provide a novel solution in the design of fat-reduced food products with no effect on the mastication parameter, sensation and perception of fat-related attributes.
34053549	1	8	theme	food	160:163	arg1	quality					149:155	the structural and sensory quality	122:155	the structural and sensory quality of food	122:163	Reducing oil/fat content without compromising the structural and sensory quality of food is a great technical challenge to the food industry.
34053549	7	9	theme	oil	803:805	arg1	oil					803:805	oil	803:805	oil	803:805	The amount of oil released as indicated by the β-carotene analysis was monitored and various influencing factors (pH, time, compositions, etc.) were also investigated.
34053549	7	9	theme	oil	803:805	arg1	amount					793:798	The amount	789:798	The amount of oil released as indicated by the β-carotene analysis	789:854	The amount of oil released as indicated by the β-carotene analysis was monitored and various influencing factors (pH, time, compositions, etc.) were also investigated.
34053549	4	10	theme	isolate	531:537	arg1	reference					597:605	a reference	595:605	a reference	595:605	On the other hand, whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier was used as a reference.
34053549	4	10	theme	isolate	531:537	arg1	emulsifier					572:581	whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier	518:581	whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier	518:581	On the other hand, whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier was used as a reference.
34053549	10	11	theme	fat-reduced	1331:1341	arg1	products					1348:1355	fat-reduced food products	1331:1355	fat-reduced food products	1331:1355	Therefore, starch emulsion could provide a novel solution in the design of fat-reduced food products with no effect on the mastication parameter, sensation and perception of fat-related attributes.
34053549	5	12	theme	emulsion	666:673	arg1	gels					675:678	emulsion gels	666:678	emulsion gels	666:678	The gellan gum was selected as a gelling agent to prepare emulsion gels.
34053549	5	13	theme	gellan	612:617	arg1	gum					619:621	The gellan gum	608:621	The gellan gum	608:621	The gellan gum was selected as a gelling agent to prepare emulsion gels.
34053549	5	13	theme	gellan	612:617	arg1	agent					649:653	a gelling agent	639:653	a gelling agent to prepare emulsion gels	639:678	The gellan gum was selected as a gelling agent to prepare emulsion gels.
34053549	7	14	theme	influencing	882:892	arg1	factors					894:900	various influencing factors	874:900	various influencing factors (pH, time, compositions, etc.)	874:931	The amount of oil released as indicated by the β-carotene analysis was monitored and various influencing factors (pH, time, compositions, etc.) were also investigated.
34053549	4	15	theme	WPI	540:542	arg1	reference					597:605	a reference	595:605	a reference	595:605	On the other hand, whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier was used as a reference.
34053549	4	15	theme	WPI	540:542	arg1	emulsifier					572:581	whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier	518:581	whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier	518:581	On the other hand, whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier was used as a reference.
34053549	8	16	theme	oiliness	1087:1094	arg1	perceptions					1072:1082	perceptions	1072:1082	perceptions	1072:1082	Using sensory panelists, oral processing of emulsion gels was examined in terms of both mastication parameters and perceptions of oiliness and thickness.
34053549	8	16	theme	oiliness	1087:1094	arg1	terms					1031:1035	terms	1031:1035	terms of both mastication parameters	1031:1066	Using sensory panelists, oral processing of emulsion gels was examined in terms of both mastication parameters and perceptions of oiliness and thickness.
34053549	9	17	theme	enhanced	1209:1216	arg1	sensation					1223:1231	an enhanced oral sensation	1206:1231	an enhanced oral sensation of oiliness mouthfeel	1206:1253	The obtained results showed that the use of a starch emulsifier gives a higher oil release and an enhanced oral sensation of oiliness mouthfeel.
34053549	6	18	theme	gels	732:735	arg1	mastication					685:695	mastication	685:695	mastication	685:695	The mastication and size reduction of the emulsion gels were examined through in-vitro and in-vivo studies.
34053549	6	18	theme	gels	732:735	arg1	reduction					706:714	size reduction	701:714	size reduction	701:714	The mastication and size reduction of the emulsion gels were examined through in-vitro and in-vivo studies.
34053549	1	19	theme	great	170:174	arg1	challenge					186:194	a great technical challenge	168:194	a great technical challenge to the food industry	168:215	Reducing oil/fat content without compromising the structural and sensory quality of food is a great technical challenge to the food industry.
34053549	9	20	theme	oral	1218:1221	arg1	sensation					1223:1231	an enhanced oral sensation	1206:1231	an enhanced oral sensation of oiliness mouthfeel	1206:1253	The obtained results showed that the use of a starch emulsifier gives a higher oil release and an enhanced oral sensation of oiliness mouthfeel.
34053549	8	21	theme	sensory	963:969	arg1	panelists					971:979	sensory panelists	963:979	sensory panelists	963:979	Using sensory panelists, oral processing of emulsion gels was examined in terms of both mastication parameters and perceptions of oiliness and thickness.
34053549	6	22	theme	emulsion	723:730	arg1	gels					732:735	the emulsion gels	719:735	the emulsion gels	719:735	The mastication and size reduction of the emulsion gels were examined through in-vitro and in-vivo studies.
34053549	0	23	theme	oral	9:12	arg1	release					18:24	oral oil release	9:24	oral oil release	9:24	Enhanced oral oil release and mouthfeel perception of starch emulsion gels.
34053549	8	24	theme	mastication	1045:1055	arg1	parameters					1057:1066	both mastication parameters	1040:1066	both mastication parameters	1040:1066	Using sensory panelists, oral processing of emulsion gels was examined in terms of both mastication parameters and perceptions of oiliness and thickness.
34053549	8	25	theme	oral	982:985	arg1	processing					987:996	oral processing	982:996	oral processing of emulsion gels	982:1013	Using sensory panelists, oral processing of emulsion gels was examined in terms of both mastication parameters and perceptions of oiliness and thickness.
34053549	7	26	theme	various	874:880	arg1	factors					894:900	various influencing factors	874:900	various influencing factors (pH, time, compositions, etc.)	874:931	The amount of oil released as indicated by the β-carotene analysis was monitored and various influencing factors (pH, time, compositions, etc.) were also investigated.
34053549	2	27	theme	emulsion	353:360	arg1	gel					362:364	an emulsion gel	350:364	an emulsion gel	350:364	The present work aims to investigate the possibility of a novel emulsion design that gives an enhanced oral release of oil/fat from an emulsion gel and therefore an enhanced mouthfeel of oiliness.
34053549	10	28	theme	mastication	1379:1389	arg1	parameter					1391:1399	the mastication parameter	1375:1399	the mastication parameter	1375:1399	Therefore, starch emulsion could provide a novel solution in the design of fat-reduced food products with no effect on the mastication parameter, sensation and perception of fat-related attributes.
34053549	6	29	theme	in-vitro	759:766	arg1	studies					780:786	in-vitro and in-vivo studies	759:786	in-vitro and in-vivo studies	759:786	The mastication and size reduction of the emulsion gels were examined through in-vitro and in-vivo studies.
34053549	2	30	theme	design	291:296	arg1	possibility					259:269	the possibility	255:269	the possibility of a novel emulsion design that gives an enhanced oral release of oil/fat from an emulsion gel and therefore an enhanced mouthfeel of oiliness	255:412	The present work aims to investigate the possibility of a novel emulsion design that gives an enhanced oral release of oil/fat from an emulsion gel and therefore an enhanced mouthfeel of oiliness.
34053549	1	31	theme	technical	176:184	arg1	challenge					186:194	a great technical challenge	168:194	a great technical challenge to the food industry	168:215	Reducing oil/fat content without compromising the structural and sensory quality of food is a great technical challenge to the food industry.
34053549	2	32	theme	oiliness	405:412	arg1	release					326:332	an enhanced oral release	309:332	an enhanced oral release of oil/fat from an emulsion gel	309:364	The present work aims to investigate the possibility of a novel emulsion design that gives an enhanced oral release of oil/fat from an emulsion gel and therefore an enhanced mouthfeel of oiliness.
34053549	2	32	theme	oiliness	405:412	arg1	mouthfeel					392:400	an enhanced mouthfeel	380:400	an enhanced mouthfeel of oiliness	380:412	The present work aims to investigate the possibility of a novel emulsion design that gives an enhanced oral release of oil/fat from an emulsion gel and therefore an enhanced mouthfeel of oiliness.
34053549	9	33	theme	oiliness	1236:1243	arg1	mouthfeel					1245:1253	oiliness mouthfeel	1236:1253	oiliness mouthfeel	1236:1253	The obtained results showed that the use of a starch emulsifier gives a higher oil release and an enhanced oral sensation of oiliness mouthfeel.
34053549	2	34	theme	emulsion	282:289	arg1	design					291:296	a novel emulsion design	274:296	a novel emulsion design that gives an enhanced oral release of oil/fat from an emulsion gel and therefore an enhanced mouthfeel of oiliness	274:412	The present work aims to investigate the possibility of a novel emulsion design that gives an enhanced oral release of oil/fat from an emulsion gel and therefore an enhanced mouthfeel of oiliness.
34053549	9	35	theme	obtained	1115:1122	arg1	results					1124:1130	The obtained results	1111:1130	The obtained results	1111:1130	The obtained results showed that the use of a starch emulsifier gives a higher oil release and an enhanced oral sensation of oiliness mouthfeel.
34053549	3	36	theme	sensitive	436:444	arg1	emulsifier					446:455	alpha-amylase sensitive emulsifier	422:455	alpha-amylase sensitive emulsifier such as starch	422:470	Hence, alpha-amylase sensitive emulsifier such as starch was used for this purpose.
34053549	1	37	theme	oil/fat	85:91	arg1	content					93:99	oil/fat content	85:99	oil/fat content	85:99	Reducing oil/fat content without compromising the structural and sensory quality of food is a great technical challenge to the food industry.
34053549	4	38	theme	protein	523:529	arg1	reference					597:605	a reference	595:605	a reference	595:605	On the other hand, whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier was used as a reference.
34053549	4	38	theme	protein	523:529	arg1	emulsifier					572:581	whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier	518:581	whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier	518:581	On the other hand, whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier was used as a reference.
34053549	5	39	theme	gelling	641:647	arg1	gum					619:621	The gellan gum	608:621	The gellan gum	608:621	The gellan gum was selected as a gelling agent to prepare emulsion gels.
34053549	5	39	theme	gelling	641:647	arg1	agent					649:653	a gelling agent	639:653	a gelling agent to prepare emulsion gels	639:678	The gellan gum was selected as a gelling agent to prepare emulsion gels.
34053549	2	40	theme	novel	276:280	arg1	design					291:296	a novel emulsion design	274:296	a novel emulsion design that gives an enhanced oral release of oil/fat from an emulsion gel and therefore an enhanced mouthfeel of oiliness	274:412	The present work aims to investigate the possibility of a novel emulsion design that gives an enhanced oral release of oil/fat from an emulsion gel and therefore an enhanced mouthfeel of oiliness.
34053549	10	41	theme	attributes	1442:1451	arg1	parameter					1391:1399	the mastication parameter	1375:1399	the mastication parameter	1375:1399	Therefore, starch emulsion could provide a novel solution in the design of fat-reduced food products with no effect on the mastication parameter, sensation and perception of fat-related attributes.
34053549	10	41	theme	attributes	1442:1451	arg1	perception					1416:1425	perception	1416:1425	perception	1416:1425	Therefore, starch emulsion could provide a novel solution in the design of fat-reduced food products with no effect on the mastication parameter, sensation and perception of fat-related attributes.
34053549	10	41	theme	attributes	1442:1451	arg1	sensation					1402:1410	sensation	1402:1410	sensation	1402:1410	Therefore, starch emulsion could provide a novel solution in the design of fat-reduced food products with no effect on the mastication parameter, sensation and perception of fat-related attributes.
34053549	2	42	theme	enhanced	383:390	arg1	mouthfeel					392:400	an enhanced mouthfeel	380:400	an enhanced mouthfeel of oiliness	380:412	The present work aims to investigate the possibility of a novel emulsion design that gives an enhanced oral release of oil/fat from an emulsion gel and therefore an enhanced mouthfeel of oiliness.
34053549	0	43	theme	mouthfeel	30:38	arg1	perception					40:49	mouthfeel perception	30:49	mouthfeel perception	30:49	Enhanced oral oil release and mouthfeel perception of starch emulsion gels.
34053549	4	44	theme	whey	518:521	arg1	reference					597:605	a reference	595:605	a reference	595:605	On the other hand, whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier was used as a reference.
34053549	4	44	theme	whey	518:521	arg1	emulsifier					572:581	whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier	518:581	whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier	518:581	On the other hand, whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier was used as a reference.
34053549	8	45	theme	gels	1010:1013	arg1	processing					987:996	oral processing	982:996	oral processing of emulsion gels	982:1013	Using sensory panelists, oral processing of emulsion gels was examined in terms of both mastication parameters and perceptions of oiliness and thickness.
34053549	2	46	theme	present	222:228	arg1	work					230:233	The present work	218:233	The present work	218:233	The present work aims to investigate the possibility of a novel emulsion design that gives an enhanced oral release of oil/fat from an emulsion gel and therefore an enhanced mouthfeel of oiliness.
34053549	9	47	theme	mouthfeel	1245:1253	arg1	release					1194:1200	a higher oil release	1181:1200	a higher oil release	1181:1200	The obtained results showed that the use of a starch emulsifier gives a higher oil release and an enhanced oral sensation of oiliness mouthfeel.
34053549	9	47	theme	mouthfeel	1245:1253	arg1	sensation					1223:1231	an enhanced oral sensation	1206:1231	an enhanced oral sensation of oiliness mouthfeel	1206:1253	The obtained results showed that the use of a starch emulsifier gives a higher oil release and an enhanced oral sensation of oiliness mouthfeel.
34053549	8	48	theme	emulsion	1001:1008	arg1	gels					1010:1013	emulsion gels	1001:1013	emulsion gels	1001:1013	Using sensory panelists, oral processing of emulsion gels was examined in terms of both mastication parameters and perceptions of oiliness and thickness.
34053549	6	49	theme	size	701:704	arg1	reduction					706:714	size reduction	701:714	size reduction	701:714	The mastication and size reduction of the emulsion gels were examined through in-vitro and in-vivo studies.
34053549	1	50	theme	food	203:206	arg1	industry					208:215	the food industry	199:215	the food industry	199:215	Reducing oil/fat content without compromising the structural and sensory quality of food is a great technical challenge to the food industry.
34053549	10	51	from	effect	1365:1370	arg1	parameter					1391:1399	the mastication parameter	1375:1399	the mastication parameter	1375:1399	Therefore, starch emulsion could provide a novel solution in the design of fat-reduced food products with no effect on the mastication parameter, sensation and perception of fat-related attributes.
34053549	10	51	from	effect	1365:1370	arg1	perception					1416:1425	perception	1416:1425	perception	1416:1425	Therefore, starch emulsion could provide a novel solution in the design of fat-reduced food products with no effect on the mastication parameter, sensation and perception of fat-related attributes.
34053549	10	51	from	effect	1365:1370	arg1	sensation					1402:1410	sensation	1402:1410	sensation	1402:1410	Therefore, starch emulsion could provide a novel solution in the design of fat-reduced food products with no effect on the mastication parameter, sensation and perception of fat-related attributes.
34053549	2	52	theme	oral	321:324	arg1	release					326:332	an enhanced oral release	309:332	an enhanced oral release of oil/fat from an emulsion gel	309:364	The present work aims to investigate the possibility of a novel emulsion design that gives an enhanced oral release of oil/fat from an emulsion gel and therefore an enhanced mouthfeel of oiliness.
34053549	4	53	theme	other	506:510	arg1	hand					512:515	the other hand	502:515	the other hand	502:515	On the other hand, whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier was used as a reference.
34053549	9	54	theme	starch	1157:1162	arg1	emulsifier					1164:1173	a starch emulsifier	1155:1173	a starch emulsifier	1155:1173	The obtained results showed that the use of a starch emulsifier gives a higher oil release and an enhanced oral sensation of oiliness mouthfeel.
34053549	9	55	theme	emulsifier	1164:1173	arg1	use					1148:1150	the use	1144:1150	the use of a starch emulsifier	1144:1173	The obtained results showed that the use of a starch emulsifier gives a higher oil release and an enhanced oral sensation of oiliness mouthfeel.
34053549	2	56	theme	enhanced	312:319	arg1	release					326:332	an enhanced oral release	309:332	an enhanced oral release of oil/fat from an emulsion gel	309:364	The present work aims to investigate the possibility of a novel emulsion design that gives an enhanced oral release of oil/fat from an emulsion gel and therefore an enhanced mouthfeel of oiliness.
34053549	7	57	theme	β-carotene	836:845	arg1	analysis					847:854	the β-carotene analysis	832:854	the β-carotene analysis	832:854	The amount of oil released as indicated by the β-carotene analysis was monitored and various influencing factors (pH, time, compositions, etc.) were also investigated.
34053549	8	58	theme	thickness	1100:1108	arg1	perceptions					1072:1082	perceptions	1072:1082	perceptions	1072:1082	Using sensory panelists, oral processing of emulsion gels was examined in terms of both mastication parameters and perceptions of oiliness and thickness.
34053549	8	58	theme	thickness	1100:1108	arg1	terms					1031:1035	terms	1031:1035	terms of both mastication parameters	1031:1066	Using sensory panelists, oral processing of emulsion gels was examined in terms of both mastication parameters and perceptions of oiliness and thickness.
34053549	6	59	theme	in-vivo	772:778	arg1	studies					780:786	in-vitro and in-vivo studies	759:786	in-vitro and in-vivo studies	759:786	The mastication and size reduction of the emulsion gels were examined through in-vitro and in-vivo studies.
34053549	2	60	from	gel	362:364	arg1	release					326:332	an enhanced oral release	309:332	an enhanced oral release of oil/fat from an emulsion gel	309:364	The present work aims to investigate the possibility of a novel emulsion design that gives an enhanced oral release of oil/fat from an emulsion gel and therefore an enhanced mouthfeel of oiliness.
34053549	2	60	from	gel	362:364	arg1	mouthfeel					392:400	an enhanced mouthfeel	380:400	an enhanced mouthfeel of oiliness	380:412	The present work aims to investigate the possibility of a novel emulsion design that gives an enhanced oral release of oil/fat from an emulsion gel and therefore an enhanced mouthfeel of oiliness.
34053549	1	61	theme	structural	126:135	arg1	quality					149:155	the structural and sensory quality	122:155	the structural and sensory quality of food	122:163	Reducing oil/fat content without compromising the structural and sensory quality of food is a great technical challenge to the food industry.
34053549	7	62	dep	factors	894:900	arg1	etc.					927:930	etc.	927:930	etc.	927:930	The amount of oil released as indicated by the β-carotene analysis was monitored and various influencing factors (pH, time, compositions, etc.) were also investigated.
34053549	7	62	dep	factors	894:900	arg1	compositions					913:924	compositions	913:924	compositions	913:924	The amount of oil released as indicated by the β-carotene analysis was monitored and various influencing factors (pH, time, compositions, etc.) were also investigated.
34053549	7	62	dep	factors	894:900	arg1	time					907:910	time	907:910	time	907:910	The amount of oil released as indicated by the β-carotene analysis was monitored and various influencing factors (pH, time, compositions, etc.) were also investigated.
34053549	7	62	dep	factors	894:900	arg1	pH					903:904	pH	903:904	pH	903:904	The amount of oil released as indicated by the β-carotene analysis was monitored and various influencing factors (pH, time, compositions, etc.) were also investigated.
34053549	4	63	theme	α-amylase	550:558	arg1	reference					597:605	a reference	595:605	a reference	595:605	On the other hand, whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier was used as a reference.
34053549	4	63	theme	α-amylase	550:558	arg1	emulsifier					572:581	whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier	518:581	whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier	518:581	On the other hand, whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier was used as a reference.
34053549	3	64	used	used	476:479	arg2	emulsifier					446:455	alpha-amylase sensitive emulsifier	422:455	alpha-amylase sensitive emulsifier such as starch	422:470	Hence, alpha-amylase sensitive emulsifier such as starch was used for this purpose.
34053549	10	65	from	solution	1305:1312	arg1	design					1321:1326	the design	1317:1326	the design of fat-reduced food products	1317:1355	Therefore, starch emulsion could provide a novel solution in the design of fat-reduced food products with no effect on the mastication parameter, sensation and perception of fat-related attributes.
34053549	4	66	theme	i.e.	545:548	arg1	reference					597:605	a reference	595:605	a reference	595:605	On the other hand, whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier was used as a reference.
34053549	4	66	theme	i.e.	545:548	arg1	emulsifier					572:581	whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier	518:581	whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier	518:581	On the other hand, whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier was used as a reference.
34053549	0	67	theme	emulsion	61:68	arg1	gels					70:73	starch emulsion gels	54:73	starch emulsion gels	54:73	Enhanced oral oil release and mouthfeel perception of starch emulsion gels.
34053549	3	68	theme	alpha-amylase	422:434	arg1	emulsifier					446:455	alpha-amylase sensitive emulsifier	422:455	alpha-amylase sensitive emulsifier such as starch	422:470	Hence, alpha-amylase sensitive emulsifier such as starch was used for this purpose.
34053549	10	69	theme	starch	1267:1272	arg1	emulsion					1274:1281	starch emulsion	1267:1281	starch emulsion	1267:1281	Therefore, starch emulsion could provide a novel solution in the design of fat-reduced food products with no effect on the mastication parameter, sensation and perception of fat-related attributes.
34053549	1	70	theme	sensory	141:147	arg1	quality					149:155	the structural and sensory quality	122:155	the structural and sensory quality of food	122:163	Reducing oil/fat content without compromising the structural and sensory quality of food is a great technical challenge to the food industry.
34053549	10	71	theme	fat-related	1430:1440	arg1	attributes					1442:1451	fat-related attributes	1430:1451	fat-related attributes	1430:1451	Therefore, starch emulsion could provide a novel solution in the design of fat-reduced food products with no effect on the mastication parameter, sensation and perception of fat-related attributes.
34053549	0	72	theme	starch	54:59	arg1	gels					70:73	starch emulsion gels	54:73	starch emulsion gels	54:73	Enhanced oral oil release and mouthfeel perception of starch emulsion gels.
34053549	9	73	theme	higher	1183:1188	arg1	release					1194:1200	a higher oil release	1181:1200	a higher oil release	1181:1200	The obtained results showed that the use of a starch emulsifier gives a higher oil release and an enhanced oral sensation of oiliness mouthfeel.
34053549	0	74	theme	oil	14:16	arg1	release					18:24	oral oil release	9:24	oral oil release	9:24	Enhanced oral oil release and mouthfeel perception of starch emulsion gels.
34053549	2	75	theme	oil/fat	337:343	arg1	release					326:332	an enhanced oral release	309:332	an enhanced oral release of oil/fat from an emulsion gel	309:364	The present work aims to investigate the possibility of a novel emulsion design that gives an enhanced oral release of oil/fat from an emulsion gel and therefore an enhanced mouthfeel of oiliness.
34053549	2	75	theme	oil/fat	337:343	arg1	mouthfeel					392:400	an enhanced mouthfeel	380:400	an enhanced mouthfeel of oiliness	380:412	The present work aims to investigate the possibility of a novel emulsion design that gives an enhanced oral release of oil/fat from an emulsion gel and therefore an enhanced mouthfeel of oiliness.
34053549	6	76	dep	mastication	685:695	arg1	The					681:683	The	681:683	The	681:683	The mastication and size reduction of the emulsion gels were examined through in-vitro and in-vivo studies.
34053549	4	77	theme	insensitive	560:570	arg1	reference					597:605	a reference	595:605	a reference	595:605	On the other hand, whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier was used as a reference.
34053549	4	77	theme	insensitive	560:570	arg1	emulsifier					572:581	whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier	518:581	whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier	518:581	On the other hand, whey protein isolate (WPI) i.e. α-amylase insensitive emulsifier was used as a reference.
34638753	0	0	theme	In-Vitro	101:108	arg1	Assessment					110:119	In-Vitro Assessment	101:119	In-Vitro Assessment	101:119	Highly Porous Fluorapatite/β-1,3-Glucan Composite for Bone Tissue Regeneration: Characterization and In-Vitro Assessment of Biomedical Potential.
34638753	8	1	theme	osteogenic	1438:1447	arg1	cells					1449:1453	osteogenic cells	1438:1453	osteogenic cells	1438:1453	The results of cell culture experiments showed that fluoride-containing biomaterial was non-toxic, enhanced the synthesis of osteocalcin and stimulated the adhesion of osteogenic cells.
34638753	7	2	theme	fluoride	1207:1214	arg1	capacity					1224:1231	optimal fluoride release capacity	1199:1231	optimal fluoride release capacity	1199:1231	Moreover, FAP/glucan was determined to be of optimal fluoride release capacity for osteoblasts growth requirements.
34638753	5	3	from	state	950:954	arg1	correlation					829:839	The correlation	825:839	The correlation between the Young's modulus and the compressive strength between the materials	825:918	The correlation between the Young's modulus and the compressive strength between the materials is different in a dry and wet state.
34638753	5	3	from	state	950:954	arg1	different					923:931	different	923:931	different	923:931	The correlation between the Young's modulus and the compressive strength between the materials is different in a dry and wet state.
34638753	8	4	theme	culture	1290:1296	arg1	experiments					1298:1308	cell culture experiments	1285:1308	cell culture experiments	1285:1308	The results of cell culture experiments showed that fluoride-containing biomaterial was non-toxic, enhanced the synthesis of osteocalcin and stimulated the adhesion of osteogenic cells.
34638753	3	5	theme	hydroxyapatite	681:694	arg1	HAP					706:708	HAP	706:708	HAP	706:708	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	3	5	theme	hydroxyapatite	681:694	arg1	granules					696:703	hydroxyapatite granules	681:703	hydroxyapatite granules (HAP)	681:709	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	1	6	theme	FAP/glucan	186:195	arg1	"					196:196	"FAP/glucan"	185:196	"FAP/glucan"	185:196	A novel fluorapatite/glucan composite ("FAP/glucan") was developed for the treatment of bone defects.
34638753	1	6	theme	FAP/glucan	186:195	arg1	composite					174:182	A novel fluorapatite/glucan composite	146:182	A novel fluorapatite/glucan composite ("FAP/glucan")	146:197	A novel fluorapatite/glucan composite ("FAP/glucan") was developed for the treatment of bone defects.
34638753	6	7	theme	reference	1134:1142	arg1	material					1144:1151	the reference material	1130:1151	the reference material	1130:1151	Bioactivity assessment showed a lower ability to form apatite and lower uptake of apatite-forming ions from the simulated body fluid by FAP/glucan material in comparison to the reference material.
34638753	5	8	theme	dry	938:940	arg1	state					950:954	a dry and wet state	936:954	a dry and wet state	936:954	The correlation between the Young's modulus and the compressive strength between the materials is different in a dry and wet state.
34638753	6	9	theme	Bioactivity	957:967	arg1	assessment					969:978	Bioactivity assessment	957:978	Bioactivity assessment	957:978	Bioactivity assessment showed a lower ability to form apatite and lower uptake of apatite-forming ions from the simulated body fluid by FAP/glucan material in comparison to the reference material.
34638753	7	10	theme	osteoblasts	1237:1247	arg1	requirements					1256:1267	osteoblasts growth requirements	1237:1267	osteoblasts growth requirements	1237:1267	Moreover, FAP/glucan was determined to be of optimal fluoride release capacity for osteoblasts growth requirements.
34638753	8	11	theme	fluoride-containing	1322:1340	arg1	biomaterial					1342:1352	fluoride-containing biomaterial	1322:1352	fluoride-containing biomaterial	1322:1352	The results of cell culture experiments showed that fluoride-containing biomaterial was non-toxic, enhanced the synthesis of osteocalcin and stimulated the adhesion of osteogenic cells.
34638753	7	12	theme	release	1216:1222	arg1	capacity					1224:1231	optimal fluoride release capacity	1199:1231	optimal fluoride release capacity	1199:1231	Moreover, FAP/glucan was determined to be of optimal fluoride release capacity for osteoblasts growth requirements.
34638753	3	13	dep	Fourier	489:495	arg1	transform					497:505	transform	497:505	transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing	497:573	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	7	14	theme	growth	1249:1254	arg1	requirements					1256:1267	osteoblasts growth requirements	1237:1267	osteoblasts growth requirements	1237:1267	Moreover, FAP/glucan was determined to be of optimal fluoride release capacity for osteoblasts growth requirements.
34638753	0	15	theme	Potential	135:143	arg1	Characterization					80:95	Characterization	80:95	Characterization	80:95	Highly Porous Fluorapatite/β-1,3-Glucan Composite for Bone Tissue Regeneration: Characterization and In-Vitro Assessment of Biomedical Potential.
34638753	0	15	theme	Potential	135:143	arg1	Assessment					110:119	In-Vitro Assessment	101:119	In-Vitro Assessment	101:119	Highly Porous Fluorapatite/β-1,3-Glucan Composite for Bone Tissue Regeneration: Characterization and In-Vitro Assessment of Biomedical Potential.
34638753	2	16	theme	polymer	286:292	arg1	presence					259:266	the presence	255:266	the presence of polysaccharide polymer (β-1,3-glucan)	255:307	Due to the presence of polysaccharide polymer (β-1,3-glucan), the composite is highly flexible and thus very convenient for surgery.
34638753	3	17	theme	scanning	453:460	arg1	SEM					483:485	SEM	483:485	SEM	483:485	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	3	17	theme	scanning	453:460	arg1	Fourier					489:495	Fourier	489:495	Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing	489:573	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	3	17	theme	scanning	453:460	arg1	microscopy					471:480	scanning electron microscopy	453:480	scanning electron microscopy (SEM)	453:486	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	0	18	theme	Biomedical	124:133	arg1	Potential					135:143	Biomedical Potential	124:143	Biomedical Potential	124:143	Highly Porous Fluorapatite/β-1,3-Glucan Composite for Bone Tissue Regeneration: Characterization and In-Vitro Assessment of Biomedical Potential.
34638753	6	19	from	apatite	1011:1017	arg1	fluid					1084:1088	the simulated body fluid	1065:1088	the simulated body fluid	1065:1088	Bioactivity assessment showed a lower ability to form apatite and lower uptake of apatite-forming ions from the simulated body fluid by FAP/glucan material in comparison to the reference material.
34638753	2	20	theme	polysaccharide	271:284	arg1	β-1,3-glucan					295:306	β-1,3-glucan	295:306	β-1,3-glucan	295:306	Due to the presence of polysaccharide polymer (β-1,3-glucan), the composite is highly flexible and thus very convenient for surgery.
34638753	2	20	theme	polysaccharide	271:284	arg1	polymer					286:292	polysaccharide polymer	271:292	polysaccharide polymer (β-1,3-glucan)	271:307	Due to the presence of polysaccharide polymer (β-1,3-glucan), the composite is highly flexible and thus very convenient for surgery.
34638753	8	21	theme	osteocalcin	1395:1405	arg1	synthesis					1382:1390	the synthesis	1378:1390	the synthesis of osteocalcin	1378:1405	The results of cell culture experiments showed that fluoride-containing biomaterial was non-toxic, enhanced the synthesis of osteocalcin and stimulated the adhesion of osteogenic cells.
34638753	3	22	theme	electron	462:469	arg1	SEM					483:485	SEM	483:485	SEM	483:485	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	3	22	theme	electron	462:469	arg1	Fourier					489:495	Fourier	489:495	Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing	489:573	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	3	22	theme	electron	462:469	arg1	microscopy					471:480	scanning electron microscopy	453:480	scanning electron microscopy (SEM)	453:486	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	0	23	theme	Porous	7:12	arg1	Composite					40:48	Highly Porous Fluorapatite/β-1,3-Glucan Composite	0:48	Highly Porous Fluorapatite/β-1,3-Glucan Composite for Bone Tissue Regeneration: Characterization and In-Vitro Assessment of Biomedical Potential.	0:144	Highly Porous Fluorapatite/β-1,3-Glucan Composite for Bone Tissue Regeneration: Characterization and In-Vitro Assessment of Biomedical Potential.
34638753	5	24	theme	Young	853:857	arg1	modulus					861:867	the Young's modulus	849:867	the Young's modulus	849:867	The correlation between the Young's modulus and the compressive strength between the materials is different in a dry and wet state.
34638753	3	25	theme	physicochemical	385:399	arg1	properties					421:430	Its physicochemical and microstructural properties	381:430	Its physicochemical and microstructural properties	381:430	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	0	26	theme	Tissue	59:64	arg1	Regeneration					66:77	Bone Tissue Regeneration	54:77	Bone Tissue Regeneration	54:77	Highly Porous Fluorapatite/β-1,3-Glucan Composite for Bone Tissue Regeneration: Characterization and In-Vitro Assessment of Biomedical Potential.
34638753	4	27	dep	higher	762:767	arg1	density					769:775	density	769:775	density	769:775	It was found that FAP/glucan has a higher density and lower porosity than the reference material.
34638753	6	28	theme	apatite-forming	1039:1053	arg1	ions					1055:1058	apatite-forming ions	1039:1058	apatite-forming ions from the simulated body fluid	1039:1088	Bioactivity assessment showed a lower ability to form apatite and lower uptake of apatite-forming ions from the simulated body fluid by FAP/glucan material in comparison to the reference material.
34638753	3	29	theme	mechanical	556:565	arg1	testing					567:573	mechanical testing	556:573	mechanical testing	556:573	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	3	29	theme	mechanical	556:565	arg1	spectroscopy					516:527	spectroscopy	516:527	spectroscopy (FTIR)	516:534	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	3	30	with	composite	651:659	arg1	HAP					706:708	HAP	706:708	HAP	706:708	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	3	30	with	composite	651:659	arg1	granules					696:703	hydroxyapatite granules	681:703	hydroxyapatite granules (HAP)	681:709	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	6	31	theme	lower	1023:1027	arg1	uptake					1029:1034	lower uptake	1023:1034	lower uptake of apatite-forming ions from the simulated body fluid by FAP/glucan material	1023:1111	Bioactivity assessment showed a lower ability to form apatite and lower uptake of apatite-forming ions from the simulated body fluid by FAP/glucan material in comparison to the reference material.
34638753	0	32	theme	Fluorapatite/β-1,3-Glucan	14:38	arg1	Composite					40:48	Highly Porous Fluorapatite/β-1,3-Glucan Composite	0:48	Highly Porous Fluorapatite/β-1,3-Glucan Composite for Bone Tissue Regeneration: Characterization and In-Vitro Assessment of Biomedical Potential.	0:144	Highly Porous Fluorapatite/β-1,3-Glucan Composite for Bone Tissue Regeneration: Characterization and In-Vitro Assessment of Biomedical Potential.
34638753	3	33	dep	transform	497:505	arg1	infrared					507:514	infrared	507:514	transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing	497:573	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	5	34	theme	compressive	877:887	arg1	strength					889:896	the compressive strength	873:896	the compressive strength	873:896	The correlation between the Young's modulus and the compressive strength between the materials is different in a dry and wet state.
34638753	8	35	theme	experiments	1298:1308	arg1	results					1274:1280	The results	1270:1280	The results of cell culture experiments	1270:1308	The results of cell culture experiments showed that fluoride-containing biomaterial was non-toxic, enhanced the synthesis of osteocalcin and stimulated the adhesion of osteogenic cells.
34638753	0	36	theme	Bone	54:57	arg1	Regeneration					66:77	Bone Tissue Regeneration	54:77	Bone Tissue Regeneration	54:77	Highly Porous Fluorapatite/β-1,3-Glucan Composite for Bone Tissue Regeneration: Characterization and In-Vitro Assessment of Biomedical Potential.
34638753	8	37	theme	cells	1449:1453	arg1	adhesion					1426:1433	the adhesion	1422:1433	the adhesion of osteogenic cells	1422:1453	The results of cell culture experiments showed that fluoride-containing biomaterial was non-toxic, enhanced the synthesis of osteocalcin and stimulated the adhesion of osteogenic cells.
34638753	3	38	theme	reference	597:605	arg1	material					607:614	the reference material	593:614	the reference material	593:614	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	3	38	theme	reference	597:605	arg1	composite					651:659	a hydroxyapatite/glucan composite	627:659	a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP	627:724	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	8	39	theme	cell	1285:1288	arg1	experiments					1298:1308	cell culture experiments	1285:1308	cell culture experiments	1285:1308	The results of cell culture experiments showed that fluoride-containing biomaterial was non-toxic, enhanced the synthesis of osteocalcin and stimulated the adhesion of osteogenic cells.
34638753	6	40	theme	ions	1055:1058	arg1	apatite					1011:1017	apatite	1011:1017	apatite	1011:1017	Bioactivity assessment showed a lower ability to form apatite and lower uptake of apatite-forming ions from the simulated body fluid by FAP/glucan material in comparison to the reference material.
34638753	6	40	theme	ions	1055:1058	arg1	uptake					1029:1034	lower uptake	1023:1034	lower uptake of apatite-forming ions from the simulated body fluid by FAP/glucan material	1023:1111	Bioactivity assessment showed a lower ability to form apatite and lower uptake of apatite-forming ions from the simulated body fluid by FAP/glucan material in comparison to the reference material.
34638753	4	41	contain	has	756:758	arg1	FAP/glucan					745:754	FAP/glucan	745:754	FAP/glucan	745:754	It was found that FAP/glucan has a higher density and lower porosity than the reference material.
34638753	4	41	contain	has	756:758	arg2	porosity					787:794	a higher density and lower porosity	760:794	a higher density and lower porosity than the reference material	760:822	It was found that FAP/glucan has a higher density and lower porosity than the reference material.
34638753	3	42	theme	hydroxyapatite/glucan	629:649	arg1	"					673:673	"HAP/glucan"	662:673	"HAP/glucan"	662:673	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	3	42	theme	hydroxyapatite/glucan	629:649	arg1	material					607:614	the reference material	593:614	the reference material	593:614	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	3	42	theme	hydroxyapatite/glucan	629:649	arg1	composite					651:659	a hydroxyapatite/glucan composite	627:659	a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP	627:724	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	4	43	theme	reference	805:813	arg1	material					815:822	the reference material	801:822	the reference material	801:822	It was found that FAP/glucan has a higher density and lower porosity than the reference material.
34638753	6	44	from	fluid	1084:1088	arg1	apatite					1011:1017	apatite	1011:1017	apatite	1011:1017	Bioactivity assessment showed a lower ability to form apatite and lower uptake of apatite-forming ions from the simulated body fluid by FAP/glucan material in comparison to the reference material.
34638753	6	44	from	fluid	1084:1088	arg1	uptake					1029:1034	lower uptake	1023:1034	lower uptake of apatite-forming ions from the simulated body fluid by FAP/glucan material	1023:1111	Bioactivity assessment showed a lower ability to form apatite and lower uptake of apatite-forming ions from the simulated body fluid by FAP/glucan material in comparison to the reference material.
34638753	6	44	from	fluid	1084:1088	arg1	ions					1055:1058	apatite-forming ions	1039:1058	apatite-forming ions from the simulated body fluid	1039:1088	Bioactivity assessment showed a lower ability to form apatite and lower uptake of apatite-forming ions from the simulated body fluid by FAP/glucan material in comparison to the reference material.
34638753	3	45	theme	microstructural	405:419	arg1	properties					421:430	Its physicochemical and microstructural properties	381:430	Its physicochemical and microstructural properties	381:430	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	6	46	theme	FAP/glucan	1093:1102	arg1	material					1104:1111	FAP/glucan material	1093:1111	FAP/glucan material	1093:1111	Bioactivity assessment showed a lower ability to form apatite and lower uptake of apatite-forming ions from the simulated body fluid by FAP/glucan material in comparison to the reference material.
34638753	0	47	dep	Composite	40:48	arg1	Characterization					80:95	Characterization	80:95	Characterization	80:95	Highly Porous Fluorapatite/β-1,3-Glucan Composite for Bone Tissue Regeneration: Characterization and In-Vitro Assessment of Biomedical Potential.
34638753	0	47	dep	Composite	40:48	arg1	Assessment					110:119	In-Vitro Assessment	101:119	In-Vitro Assessment	101:119	Highly Porous Fluorapatite/β-1,3-Glucan Composite for Bone Tissue Regeneration: Characterization and In-Vitro Assessment of Biomedical Potential.
34638753	6	48	from	uptake	1029:1034	arg1	fluid					1084:1088	the simulated body fluid	1065:1088	the simulated body fluid	1065:1088	Bioactivity assessment showed a lower ability to form apatite and lower uptake of apatite-forming ions from the simulated body fluid by FAP/glucan material in comparison to the reference material.
34638753	1	49	theme	novel	148:152	arg1	"					196:196	"FAP/glucan"	185:196	"FAP/glucan"	185:196	A novel fluorapatite/glucan composite ("FAP/glucan") was developed for the treatment of bone defects.
34638753	1	49	theme	novel	148:152	arg1	composite					174:182	A novel fluorapatite/glucan composite	146:182	A novel fluorapatite/glucan composite ("FAP/glucan")	146:197	A novel fluorapatite/glucan composite ("FAP/glucan") was developed for the treatment of bone defects.
34638753	1	50	theme	bone	234:237	arg1	defects					239:245	bone defects	234:245	bone defects	234:245	A novel fluorapatite/glucan composite ("FAP/glucan") was developed for the treatment of bone defects.
34638753	3	51	theme	mercury	537:543	arg1	intrusion					545:553	mercury intrusion	537:553	mercury intrusion	537:553	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	3	51	theme	mercury	537:543	arg1	spectroscopy					516:527	spectroscopy	516:527	spectroscopy (FTIR)	516:534	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	3	52	theme	HAP/glucan	663:672	arg1	"					673:673	"HAP/glucan"	662:673	"HAP/glucan"	662:673	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	3	52	theme	HAP/glucan	663:672	arg1	composite					651:659	a hydroxyapatite/glucan composite	627:659	a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP	627:724	Its physicochemical and microstructural properties were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), mercury intrusion, mechanical testing and compared with the reference material, which was a hydroxyapatite/glucan composite ("HAP/glucan") with hydroxyapatite granules (HAP) instead of FAP.
34638753	4	53	theme	higher	762:767	arg1	porosity					787:794	a higher density and lower porosity	760:794	a higher density and lower porosity than the reference material	760:822	It was found that FAP/glucan has a higher density and lower porosity than the reference material.
34638753	1	54	theme	fluorapatite/glucan	154:172	arg1	"					196:196	"FAP/glucan"	185:196	"FAP/glucan"	185:196	A novel fluorapatite/glucan composite ("FAP/glucan") was developed for the treatment of bone defects.
34638753	1	54	theme	fluorapatite/glucan	154:172	arg1	composite					174:182	A novel fluorapatite/glucan composite	146:182	A novel fluorapatite/glucan composite ("FAP/glucan")	146:197	A novel fluorapatite/glucan composite ("FAP/glucan") was developed for the treatment of bone defects.
34638753	1	55	theme	defects	239:245	arg1	treatment					221:229	the treatment	217:229	the treatment of bone defects	217:245	A novel fluorapatite/glucan composite ("FAP/glucan") was developed for the treatment of bone defects.
34638753	5	56	theme	wet	946:948	arg1	state					950:954	a dry and wet state	936:954	a dry and wet state	936:954	The correlation between the Young's modulus and the compressive strength between the materials is different in a dry and wet state.
34638753	4	57	theme	lower	781:785	arg1	porosity					787:794	a higher density and lower porosity	760:794	a higher density and lower porosity than the reference material	760:822	It was found that FAP/glucan has a higher density and lower porosity than the reference material.
34638753	6	58	theme	body	1079:1082	arg1	fluid					1084:1088	the simulated body fluid	1065:1088	the simulated body fluid	1065:1088	Bioactivity assessment showed a lower ability to form apatite and lower uptake of apatite-forming ions from the simulated body fluid by FAP/glucan material in comparison to the reference material.
34638753	5	59	from	different	923:931	arg1	state					950:954	a dry and wet state	936:954	a dry and wet state	936:954	The correlation between the Young's modulus and the compressive strength between the materials is different in a dry and wet state.
34638753	6	60	theme	lower	989:993	arg1	ability					995:1001	a lower ability	987:1001	a lower ability to form apatite and lower uptake of apatite-forming ions from the simulated body fluid by FAP/glucan material in comparison to the reference material	987:1151	Bioactivity assessment showed a lower ability to form apatite and lower uptake of apatite-forming ions from the simulated body fluid by FAP/glucan material in comparison to the reference material.
34638753	7	61	theme	optimal	1199:1205	arg1	capacity					1224:1231	optimal fluoride release capacity	1199:1231	optimal fluoride release capacity	1199:1231	Moreover, FAP/glucan was determined to be of optimal fluoride release capacity for osteoblasts growth requirements.
34638753	6	62	theme	simulated	1069:1077	arg1	fluid					1084:1088	the simulated body fluid	1065:1088	the simulated body fluid	1065:1088	Bioactivity assessment showed a lower ability to form apatite and lower uptake of apatite-forming ions from the simulated body fluid by FAP/glucan material in comparison to the reference material.
33483050	4	0	theme	CHC-CMC	955:961	arg1	nanoparticles					963:975	the CHC-CMC nanoparticles	951:975	the CHC-CMC nanoparticles	951:975	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	4	0	theme	CHC-CMC	955:961	arg1	adsorbing					990:998	adsorbing	990:998	adsorbing	990:998	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	4	1	theme	scanning	842:849	arg1	microscopy					851:860	Confocal laser scanning microscopy	827:860	Confocal laser scanning microscopy	827:860	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	4	2	dep	d.	824:825	arg1	confirmed					862:870	confirmed	862:870	confirmed	862:870	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	4	3	theme	%	716:716	arg1	index					748:752	the creaming index	735:752	the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface	735:1025	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	4	3	theme	%	716:716	arg1	alginate					725:732	1 wt % sodium alginate	711:732	1 wt % sodium alginate	711:732	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	4	4	dep	higher	767:772	arg1	%					812:812	67.4 %	807:812	67.4 %	807:812	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	4	5	theme	CHC-CMC	587:593	arg1	formation					595:603	CHC-CMC formation	587:603	CHC-CMC formation	587:603	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	3	6	theme	CMC	568:570	arg1	ratio					572:576	the CHC: CMC ratio	559:576	the CHC: CMC ratio	559:576	CHC-CMC nanoparticles (261 nm) were obtained when the CHC: CMC ratio was 1:2.
33483050	4	7	theme	wt	713:714	arg1	index					748:752	the creaming index	735:752	the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface	735:1025	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	4	7	theme	wt	713:714	arg1	alginate					725:732	1 wt % sodium alginate	711:732	1 wt % sodium alginate	711:732	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	3	8	theme	CHC-CMC	509:515	arg1	nanoparticles					517:529	CHC-CMC nanoparticles	509:529	CHC-CMC nanoparticles (261 nm)	509:538	CHC-CMC nanoparticles (261 nm) were obtained when the CHC: CMC ratio was 1:2.
33483050	3	8	theme	CHC-CMC	509:515	arg1	nm					536:537	261 nm	532:537	261 nm	532:537	CHC-CMC nanoparticles (261 nm) were obtained when the CHC: CMC ratio was 1:2.
33483050	4	9	with	collapse	919:926	arg1	Tween					933:937	Tween	933:937	Tween	933:937	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	1	10	theme	nanoparticles	177:189	arg1	role					169:172	the role	165:172	the role of nanoparticles in stabilizing the interface	165:218	For preparing stable water-in-oil-in-water emulsion, the role of nanoparticles in stabilizing the interface is very important.
33483050	1	10	theme	nanoparticles	177:189	arg1	important					228:236	important	228:236	important	228:236	For preparing stable water-in-oil-in-water emulsion, the role of nanoparticles in stabilizing the interface is very important.
33483050	4	11	theme	sodium	718:723	arg1	index					748:752	the creaming index	735:752	the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface	735:1025	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	4	11	theme	sodium	718:723	arg1	alginate					725:732	1 wt % sodium alginate	711:732	1 wt % sodium alginate	711:732	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	0	12	theme	composite	2:10	arg1	nanoparticle					32:43	A composite chitosan derivative nanoparticle	0:43	A composite chitosan derivative nanoparticle	0:43	A composite chitosan derivative nanoparticle to stabilize a W1/O/W2 emulsion: Preparation and characterization.
33483050	2	13	theme	electrostatic	350:362	arg1	interactions					364:375	electrostatic interactions	350:375	electrostatic interactions	350:375	In this study, chitosan hydrochloride-carboxymethyl chitosan (CHC-CMC) nanoparticles were prepared considering electrostatic interactions; then the emulsion was prepared and the stability characteristics in presence of NaCl (0-200 mmol/L) and 30 d storage were studied.
33483050	2	14	theme	stability	417:425	arg1	characteristics					427:441	the stability characteristics	413:441	the stability characteristics in presence of NaCl (0-200 mmol/L) and 30 d storage	413:493	In this study, chitosan hydrochloride-carboxymethyl chitosan (CHC-CMC) nanoparticles were prepared considering electrostatic interactions; then the emulsion was prepared and the stability characteristics in presence of NaCl (0-200 mmol/L) and 30 d storage were studied.
33483050	4	15	contain	contained	701:709	arg1	W2					666:667	W2	666:667	W2	666:667	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	4	15	contain	contained	701:709	arg2	alginate					725:732	1 wt % sodium alginate	711:732	1 wt % sodium alginate	711:732	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	4	15	contain	contained	701:709	arg2	index					748:752	the creaming index	735:752	the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface	735:1025	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	4	15	contain	contained	701:709	arg1	W1					698:699	W1	698:699	W1	698:699	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	4	16	dep	collapse	919:926	arg1	contrast					903:910	contrast	903:910	contrast	903:910	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	0	17	theme	derivative	21:30	arg1	nanoparticle					32:43	A composite chitosan derivative nanoparticle	0:43	A composite chitosan derivative nanoparticle	0:43	A composite chitosan derivative nanoparticle to stabilize a W1/O/W2 emulsion: Preparation and characterization.
33483050	3	18	theme	CHC	563:565	arg1	ratio					572:576	the CHC: CMC ratio	559:576	the CHC: CMC ratio	559:576	CHC-CMC nanoparticles (261 nm) were obtained when the CHC: CMC ratio was 1:2.
33483050	0	19	theme	chitosan	12:19	arg1	nanoparticle					32:43	A composite chitosan derivative nanoparticle	0:43	A composite chitosan derivative nanoparticle	0:43	A composite chitosan derivative nanoparticle to stabilize a W1/O/W2 emulsion: Preparation and characterization.
33483050	2	20	theme	NaCl	458:461	arg1	presence					446:453	presence	446:453	presence of NaCl (0-200 mmol/L) and 30 d storage	446:493	In this study, chitosan hydrochloride-carboxymethyl chitosan (CHC-CMC) nanoparticles were prepared considering electrostatic interactions; then the emulsion was prepared and the stability characteristics in presence of NaCl (0-200 mmol/L) and 30 d storage were studied.
33483050	5	21	theme	W1/O/W2	1092:1098	arg1	emulsion					1100:1107	W1/O/W2 emulsion	1092:1107	W1/O/W2 emulsion	1092:1107	This indicates that CHC-CMC has a stronger ability to stabilize W1/O/W2 emulsion than Tween 80.
33483050	4	22	theme	Confocal	827:834	arg1	microscopy					851:860	Confocal laser scanning microscopy	827:860	Confocal laser scanning microscopy	827:860	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	4	23	theme	oil-water	1007:1015	arg1	interface					1017:1025	the oil-water interface	1003:1025	the oil-water interface	1003:1025	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	4	24	theme	new	634:636	arg1	peak					638:641	a new peak	632:641	a new peak	632:641	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	2	25	theme	storage	487:493	arg1	presence					446:453	presence	446:453	presence of NaCl (0-200 mmol/L) and 30 d storage	446:493	In this study, chitosan hydrochloride-carboxymethyl chitosan (CHC-CMC) nanoparticles were prepared considering electrostatic interactions; then the emulsion was prepared and the stability characteristics in presence of NaCl (0-200 mmol/L) and 30 d storage were studied.
33483050	0	26	dep	stabilize	48:56	arg1	characterization					94:109	characterization	94:109	characterization	94:109	A composite chitosan derivative nanoparticle to stabilize a W1/O/W2 emulsion: Preparation and characterization.
33483050	0	26	dep	stabilize	48:56	arg1	Preparation					78:88	Preparation	78:88	Preparation	78:88	A composite chitosan derivative nanoparticle to stabilize a W1/O/W2 emulsion: Preparation and characterization.
33483050	2	27	theme	d	485:485	arg1	storage					487:493	30 d storage	482:493	30 d storage	482:493	In this study, chitosan hydrochloride-carboxymethyl chitosan (CHC-CMC) nanoparticles were prepared considering electrostatic interactions; then the emulsion was prepared and the stability characteristics in presence of NaCl (0-200 mmol/L) and 30 d storage were studied.
33483050	5	28	contain	has	1056:1058	arg1	CHC-CMC					1048:1054	CHC-CMC	1048:1054	CHC-CMC	1048:1054	This indicates that CHC-CMC has a stronger ability to stabilize W1/O/W2 emulsion than Tween 80.
33483050	5	28	contain	has	1056:1058	arg2	ability					1071:1077	a stronger ability	1060:1077	a stronger ability to stabilize W1/O/W2 emulsion	1060:1107	This indicates that CHC-CMC has a stronger ability to stabilize W1/O/W2 emulsion than Tween 80.
33483050	2	29	dep	prepared	329:336	arg1	prepared					400:407	prepared	400:407	was prepared	396:407	In this study, chitosan hydrochloride-carboxymethyl chitosan (CHC-CMC) nanoparticles were prepared considering electrostatic interactions; then the emulsion was prepared and the stability characteristics in presence of NaCl (0-200 mmol/L) and 30 d storage were studied.
33483050	1	30	theme	stable	126:131	arg1	emulsion					155:162	stable water-in-oil-in-water emulsion	126:162	stable water-in-oil-in-water emulsion	126:162	For preparing stable water-in-oil-in-water emulsion, the role of nanoparticles in stabilizing the interface is very important.
33483050	5	31	theme	stronger	1062:1069	arg1	ability					1071:1077	a stronger ability	1060:1077	a stronger ability to stabilize W1/O/W2 emulsion	1060:1107	This indicates that CHC-CMC has a stronger ability to stabilize W1/O/W2 emulsion than Tween 80.
33483050	4	32	theme	wt	681:682	arg1	CHC-CMC					686:692	2 wt % CHC-CMC	679:692	2 wt % CHC-CMC	679:692	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	2	33	theme	chitosan	291:298	arg1	nanoparticles					310:322	chitosan hydrochloride-carboxymethyl chitosan (CHC-CMC) nanoparticles	254:322	chitosan hydrochloride-carboxymethyl chitosan (CHC-CMC) nanoparticles	254:322	In this study, chitosan hydrochloride-carboxymethyl chitosan (CHC-CMC) nanoparticles were prepared considering electrostatic interactions; then the emulsion was prepared and the stability characteristics in presence of NaCl (0-200 mmol/L) and 30 d storage were studied.
33483050	2	34	from	characteristics	427:441	arg1	presence					446:453	presence	446:453	presence of NaCl (0-200 mmol/L) and 30 d storage	446:493	In this study, chitosan hydrochloride-carboxymethyl chitosan (CHC-CMC) nanoparticles were prepared considering electrostatic interactions; then the emulsion was prepared and the stability characteristics in presence of NaCl (0-200 mmol/L) and 30 d storage were studied.
33483050	1	35	theme	water-in-oil-in-water	133:153	arg1	emulsion					155:162	stable water-in-oil-in-water emulsion	126:162	stable water-in-oil-in-water emulsion	126:162	For preparing stable water-in-oil-in-water emulsion, the role of nanoparticles in stabilizing the interface is very important.
33483050	2	36	theme	hydrochloride-carboxymethyl	263:289	arg1	CHC-CMC					301:307	CHC-CMC	301:307	CHC-CMC	301:307	In this study, chitosan hydrochloride-carboxymethyl chitosan (CHC-CMC) nanoparticles were prepared considering electrostatic interactions; then the emulsion was prepared and the stability characteristics in presence of NaCl (0-200 mmol/L) and 30 d storage were studied.
33483050	2	36	theme	hydrochloride-carboxymethyl	263:289	arg1	chitosan					291:298	chitosan hydrochloride-carboxymethyl chitosan	254:298	chitosan hydrochloride-carboxymethyl chitosan (CHC-CMC) nanoparticles	254:322	In this study, chitosan hydrochloride-carboxymethyl chitosan (CHC-CMC) nanoparticles were prepared considering electrostatic interactions; then the emulsion was prepared and the stability characteristics in presence of NaCl (0-200 mmol/L) and 30 d storage were studied.
33483050	4	37	theme	%	684:684	arg1	CHC-CMC					686:692	2 wt % CHC-CMC	679:692	2 wt % CHC-CMC	679:692	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	4	38	theme	double	876:881	arg1	microstructures					883:897	the double microstructures	872:897	the double microstructures	872:897	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	2	39	theme	chitosan	254:261	arg1	CHC-CMC					301:307	CHC-CMC	301:307	CHC-CMC	301:307	In this study, chitosan hydrochloride-carboxymethyl chitosan (CHC-CMC) nanoparticles were prepared considering electrostatic interactions; then the emulsion was prepared and the stability characteristics in presence of NaCl (0-200 mmol/L) and 30 d storage were studied.
33483050	2	39	theme	chitosan	254:261	arg1	chitosan					291:298	chitosan hydrochloride-carboxymethyl chitosan	254:298	chitosan hydrochloride-carboxymethyl chitosan (CHC-CMC) nanoparticles	254:322	In this study, chitosan hydrochloride-carboxymethyl chitosan (CHC-CMC) nanoparticles were prepared considering electrostatic interactions; then the emulsion was prepared and the stability characteristics in presence of NaCl (0-200 mmol/L) and 30 d storage were studied.
33483050	0	40	theme	W1/O/W2	60:66	arg1	emulsion					68:75	a W1/O/W2 emulsion	58:75	a W1/O/W2 emulsion	58:75	A composite chitosan derivative nanoparticle to stabilize a W1/O/W2 emulsion: Preparation and characterization.
33483050	4	41	theme	creaming	739:746	arg1	index					748:752	the creaming index	735:752	the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface	735:1025	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	4	41	theme	creaming	739:746	arg1	%					760:760	81.6 %	755:760	81.6 %	755:760	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	4	41	theme	creaming	739:746	arg1	alginate					725:732	1 wt % sodium alginate	711:732	1 wt % sodium alginate	711:732	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33483050	4	42	theme	laser	836:840	arg1	microscopy					851:860	Confocal laser scanning microscopy	827:860	Confocal laser scanning microscopy	827:860	CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm-1; W2 contained 2 wt % CHC-CMC and W1 contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface.
33761468	4	0	contain	having	717:722	arg1	scaffolds					707:715	scaffolds	707:715	scaffolds having only microporosity (GMi) and macroporosity (GMa)	707:771	These multiscale porous scaffolds (GMu) were compared with two other types of scaffolds having only microporosity (GMi) and macroporosity (GMa) with regard to their physical andin vitrobiological properties.
33761468	4	0	contain	having	717:722	arg2	GMi					744:746	GMi	744:746	GMi	744:746	These multiscale porous scaffolds (GMu) were compared with two other types of scaffolds having only microporosity (GMi) and macroporosity (GMa) with regard to their physical andin vitrobiological properties.
33761468	4	0	contain	having	717:722	arg2	microporosity					729:741	only microporosity	724:741	only microporosity (GMi)	724:747	These multiscale porous scaffolds (GMu) were compared with two other types of scaffolds having only microporosity (GMi) and macroporosity (GMa) with regard to their physical andin vitrobiological properties.
33761468	4	0	contain	having	717:722	arg2	GMa					768:770	GMa	768:770	GMa	768:770	These multiscale porous scaffolds (GMu) were compared with two other types of scaffolds having only microporosity (GMi) and macroporosity (GMa) with regard to their physical andin vitrobiological properties.
33761468	4	0	contain	having	717:722	arg2	macroporosity					753:765	macroporosity	753:765	macroporosity (GMa)	753:771	These multiscale porous scaffolds (GMu) were compared with two other types of scaffolds having only microporosity (GMi) and macroporosity (GMa) with regard to their physical andin vitrobiological properties.
33761468	1	1	theme	complex-shaped	128:141	arg1	scaffold					168:175	a complex-shaped gelatin-gellan composite scaffold	126:175	a complex-shaped gelatin-gellan composite scaffold with multiscale porosity	126:200	The aim of this work was to develop a complex-shaped gelatin-gellan composite scaffold with multiscale porosity using a combination of cryogenic 3D printing and lyophilization for bone tissue engineering.
33761468	0	2	theme	tissue	70:75	arg1	engineering					77:87	bone tissue engineering	65:87	bone tissue engineering	65:87	Multiscale porosity in a 3D printed gellan-gelatin composite for bone tissue engineering.
33761468	0	3	from	porosity	11:18	arg1	gellan-gelatin					36:49	a 3D printed gellan-gelatin	23:49	a 3D printed gellan-gelatin composite for bone tissue engineering	23:87	Multiscale porosity in a 3D printed gellan-gelatin composite for bone tissue engineering.
33761468	1	4	theme	gelatin-gellan	143:156	arg1	scaffold					168:175	a complex-shaped gelatin-gellan composite scaffold	126:175	a complex-shaped gelatin-gellan composite scaffold with multiscale porosity	126:200	The aim of this work was to develop a complex-shaped gelatin-gellan composite scaffold with multiscale porosity using a combination of cryogenic 3D printing and lyophilization for bone tissue engineering.
33761468	5	5	theme	cellular	970:977	arg1	infiltration					979:990	cellular infiltration	970:990	cellular infiltration	970:990	GMu scaffolds were found to be better than GMi and GMa in terms of swelling percentage, degradation rate, uniform pore distribution, cellular infiltration, attachment, proliferation, protein generation and mineralization.
33761468	5	5	theme	cellular	970:977	arg1	terms					895:899	terms	895:899	terms of swelling percentage	895:922	GMu scaffolds were found to be better than GMi and GMa in terms of swelling percentage, degradation rate, uniform pore distribution, cellular infiltration, attachment, proliferation, protein generation and mineralization.
33761468	4	6	theme	porous	646:651	arg1	GMu					664:666	GMu	664:666	GMu	664:666	These multiscale porous scaffolds (GMu) were compared with two other types of scaffolds having only microporosity (GMi) and macroporosity (GMa) with regard to their physical andin vitrobiological properties.
33761468	4	6	theme	porous	646:651	arg1	scaffolds					653:661	These multiscale porous scaffolds	629:661	These multiscale porous scaffolds (GMu)	629:667	These multiscale porous scaffolds (GMu) were compared with two other types of scaffolds having only microporosity (GMi) and macroporosity (GMa) with regard to their physical andin vitrobiological properties.
33761468	5	7	theme	protein	1020:1026	arg1	terms					895:899	terms	895:899	terms of swelling percentage	895:922	GMu scaffolds were found to be better than GMi and GMa in terms of swelling percentage, degradation rate, uniform pore distribution, cellular infiltration, attachment, proliferation, protein generation and mineralization.
33761468	5	7	theme	protein	1020:1026	arg1	generation					1028:1037	protein generation	1020:1037	protein generation	1020:1037	GMu scaffolds were found to be better than GMi and GMa in terms of swelling percentage, degradation rate, uniform pore distribution, cellular infiltration, attachment, proliferation, protein generation and mineralization.
33761468	2	8	theme	Cryogenic	295:303	arg1	printing					308:315	Cryogenic 3D printing	295:315	Cryogenic 3D printing	295:315	Cryogenic 3D printing was used to fabricate a low-concentration composite of complex-shaped macroporous gelatin-gellan structures with a pore size of 919 ± 89 µm.
33761468	5	9	theme	uniform	943:949	arg1	distribution					956:967	uniform pore distribution	943:967	uniform pore distribution	943:967	GMu scaffolds were found to be better than GMi and GMa in terms of swelling percentage, degradation rate, uniform pore distribution, cellular infiltration, attachment, proliferation, protein generation and mineralization.
33761468	5	9	theme	uniform	943:949	arg1	terms					895:899	terms	895:899	terms of swelling percentage	895:922	GMu scaffolds were found to be better than GMi and GMa in terms of swelling percentage, degradation rate, uniform pore distribution, cellular infiltration, attachment, proliferation, protein generation and mineralization.
33761468	5	10	theme	pore	951:954	arg1	distribution					956:967	uniform pore distribution	943:967	uniform pore distribution	943:967	GMu scaffolds were found to be better than GMi and GMa in terms of swelling percentage, degradation rate, uniform pore distribution, cellular infiltration, attachment, proliferation, protein generation and mineralization.
33761468	5	10	theme	pore	951:954	arg1	terms					895:899	terms	895:899	terms of swelling percentage	895:922	GMu scaffolds were found to be better than GMi and GMa in terms of swelling percentage, degradation rate, uniform pore distribution, cellular infiltration, attachment, proliferation, protein generation and mineralization.
33761468	6	11	theme	reproducible	1182:1193	arg1	process					1195:1201	a reproducible process	1180:1201	a reproducible process of manufacture	1180:1216	In conclusion, we have developed a controlled hierarchical bone-like structure, biomimicking natural bone, together with a reproducible process of manufacture by coupling soft hydrogel 3D printing with lyophilization.
33761468	3	12	theme	functionalities	564:578	arg1	micropores					507:516	micropores	507:516	micropores of size 20-250 µm and nanometre-level surface functionalities	507:578	This was followed by lyophilization to introduce micropores of size 20-250 µm and nanometre-level surface functionalities, thus achieving a hierarchical porous structure.
33761468	1	13	theme	bone	270:273	arg1	engineering					282:292	bone tissue engineering	270:292	bone tissue engineering	270:292	The aim of this work was to develop a complex-shaped gelatin-gellan composite scaffold with multiscale porosity using a combination of cryogenic 3D printing and lyophilization for bone tissue engineering.
33761468	4	14	theme	scaffolds	707:715	arg1	types					698:702	two other types	688:702	two other types of scaffolds having only microporosity (GMi) and macroporosity (GMa)	688:771	These multiscale porous scaffolds (GMu) were compared with two other types of scaffolds having only microporosity (GMi) and macroporosity (GMa) with regard to their physical andin vitrobiological properties.
33761468	0	15	from	gellan-gelatin	36:49	arg1	porosity					11:18	porosity	11:18	porosity	11:18	Multiscale porosity in a 3D printed gellan-gelatin composite for bone tissue engineering.
33761468	7	16	with	scaffolds	1361:1369	arg1	potential					1414:1422	enhanced performancein vitroand great potential	1376:1422	enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine	1376:1497	This enables the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine.
33761468	7	17	theme	printed	1344:1350	arg1	scaffolds					1361:1369	complex-shaped patient-specific 3D printed hydrogel scaffolds	1309:1369	complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine	1309:1497	This enables the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine.
33761468	4	18	theme	other	692:696	arg1	types					698:702	two other types	688:702	two other types of scaffolds having only microporosity (GMi) and macroporosity (GMa)	688:771	These multiscale porous scaffolds (GMu) were compared with two other types of scaffolds having only microporosity (GMi) and macroporosity (GMa) with regard to their physical andin vitrobiological properties.
33761468	1	19	theme	composite	158:166	arg1	scaffold					168:175	a complex-shaped gelatin-gellan composite scaffold	126:175	a complex-shaped gelatin-gellan composite scaffold with multiscale porosity	126:200	The aim of this work was to develop a complex-shaped gelatin-gellan composite scaffold with multiscale porosity using a combination of cryogenic 3D printing and lyophilization for bone tissue engineering.
33761468	3	20	theme	nanometre-level	540:554	arg1	functionalities					564:578	nanometre-level surface functionalities	540:578	nanometre-level surface functionalities	540:578	This was followed by lyophilization to introduce micropores of size 20-250 µm and nanometre-level surface functionalities, thus achieving a hierarchical porous structure.
33761468	7	21	theme	enhanced	1376:1383	arg1	potential					1414:1422	enhanced performancein vitroand great potential	1376:1422	enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine	1376:1497	This enables the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine.
33761468	7	22	theme	performancein	1385:1397	arg1	potential					1414:1422	enhanced performancein vitroand great potential	1376:1422	enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine	1376:1497	This enables the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine.
33761468	5	23	theme	degradation	925:935	arg1	terms					895:899	terms	895:899	terms of swelling percentage	895:922	GMu scaffolds were found to be better than GMi and GMa in terms of swelling percentage, degradation rate, uniform pore distribution, cellular infiltration, attachment, proliferation, protein generation and mineralization.
33761468	5	23	theme	degradation	925:935	arg1	rate					937:940	degradation rate	925:940	degradation rate	925:940	GMu scaffolds were found to be better than GMi and GMa in terms of swelling percentage, degradation rate, uniform pore distribution, cellular infiltration, attachment, proliferation, protein generation and mineralization.
33761468	7	24	from	potential	1414:1422	arg1	medicine					1490:1497	regenerative medicine	1477:1497	regenerative medicine	1477:1497	This enables the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine.
33761468	7	24	from	potential	1414:1422	arg1	bioprinting					1461:1471	bioprinting	1461:1471	bioprinting	1461:1471	This enables the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine.
33761468	7	24	from	potential	1414:1422	arg1	fields					1431:1436	the fields	1427:1436	the fields of tissue engineering	1427:1458	This enables the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine.
33761468	2	25	theme	structures	414:423	arg1	structures					414:423	complex-shaped macroporous gelatin-gellan structures	372:423	complex-shaped macroporous gelatin-gellan structures	372:423	Cryogenic 3D printing was used to fabricate a low-concentration composite of complex-shaped macroporous gelatin-gellan structures with a pore size of 919 ± 89 µm.
33761468	2	25	theme	structures	414:423	arg1	composite					359:367	a low-concentration composite	339:367	a low-concentration composite of complex-shaped macroporous gelatin-gellan structures	339:423	Cryogenic 3D printing was used to fabricate a low-concentration composite of complex-shaped macroporous gelatin-gellan structures with a pore size of 919 ± 89 µm.
33761468	2	26	theme	µm	454:455	arg1	size					437:440	a pore size	430:440	a pore size of 919 ± 89 µm	430:455	Cryogenic 3D printing was used to fabricate a low-concentration composite of complex-shaped macroporous gelatin-gellan structures with a pore size of 919 ± 89 µm.
33761468	1	27	theme	tissue	275:280	arg1	engineering					282:292	bone tissue engineering	270:292	bone tissue engineering	270:292	The aim of this work was to develop a complex-shaped gelatin-gellan composite scaffold with multiscale porosity using a combination of cryogenic 3D printing and lyophilization for bone tissue engineering.
33761468	7	28	theme	great	1408:1412	arg1	potential					1414:1422	enhanced performancein vitroand great potential	1376:1422	enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine	1376:1497	This enables the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine.
33761468	7	29	theme	patient-specific	1324:1339	arg1	scaffolds					1361:1369	complex-shaped patient-specific 3D printed hydrogel scaffolds	1309:1369	complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine	1309:1497	This enables the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine.
33761468	2	30	theme	gelatin-gellan	399:412	arg1	structures					414:423	complex-shaped macroporous gelatin-gellan structures	372:423	complex-shaped macroporous gelatin-gellan structures	372:423	Cryogenic 3D printing was used to fabricate a low-concentration composite of complex-shaped macroporous gelatin-gellan structures with a pore size of 919 ± 89 µm.
33761468	6	31	theme	manufacture	1206:1216	arg1	process					1195:1201	a reproducible process	1180:1201	a reproducible process of manufacture	1180:1216	In conclusion, we have developed a controlled hierarchical bone-like structure, biomimicking natural bone, together with a reproducible process of manufacture by coupling soft hydrogel 3D printing with lyophilization.
33761468	6	32	theme	soft	1230:1233	arg1	printing					1247:1254	coupling soft hydrogel 3D printing	1221:1254	coupling soft hydrogel 3D printing with lyophilization	1221:1274	In conclusion, we have developed a controlled hierarchical bone-like structure, biomimicking natural bone, together with a reproducible process of manufacture by coupling soft hydrogel 3D printing with lyophilization.
33761468	7	33	theme	tissue	1441:1446	arg1	engineering					1448:1458	tissue engineering	1441:1458	tissue engineering	1441:1458	This enables the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine.
33761468	2	34	theme	919	445:447	arg1	µm					454:455	919 ± 89 µm	445:455	919 ± 89 µm	445:455	Cryogenic 3D printing was used to fabricate a low-concentration composite of complex-shaped macroporous gelatin-gellan structures with a pore size of 919 ± 89 µm.
33761468	2	35	theme	±	449:449	arg1	µm					454:455	919 ± 89 µm	445:455	919 ± 89 µm	445:455	Cryogenic 3D printing was used to fabricate a low-concentration composite of complex-shaped macroporous gelatin-gellan structures with a pore size of 919 ± 89 µm.
33761468	2	36	theme	3D	305:306	arg1	printing					308:315	Cryogenic 3D printing	295:315	Cryogenic 3D printing	295:315	Cryogenic 3D printing was used to fabricate a low-concentration composite of complex-shaped macroporous gelatin-gellan structures with a pore size of 919 ± 89 µm.
33761468	6	37	theme	hierarchical	1105:1116	arg1	structure					1128:1136	a controlled hierarchical bone-like structure	1092:1136	a controlled hierarchical bone-like structure	1092:1136	In conclusion, we have developed a controlled hierarchical bone-like structure, biomimicking natural bone, together with a reproducible process of manufacture by coupling soft hydrogel 3D printing with lyophilization.
33761468	0	38	theme	printed	28:34	arg1	gellan-gelatin					36:49	a 3D printed gellan-gelatin	23:49	a 3D printed gellan-gelatin composite for bone tissue engineering	23:87	Multiscale porosity in a 3D printed gellan-gelatin composite for bone tissue engineering.
33761468	4	39	theme	only	724:727	arg1	microporosity					729:741	only microporosity	724:741	only microporosity (GMi)	724:747	These multiscale porous scaffolds (GMu) were compared with two other types of scaffolds having only microporosity (GMi) and macroporosity (GMa) with regard to their physical andin vitrobiological properties.
33761468	4	39	theme	only	724:727	arg1	GMi					744:746	GMi	744:746	GMi	744:746	These multiscale porous scaffolds (GMu) were compared with two other types of scaffolds having only microporosity (GMi) and macroporosity (GMa) with regard to their physical andin vitrobiological properties.
33761468	6	40	theme	hydrogel	1235:1242	arg1	printing					1247:1254	coupling soft hydrogel 3D printing	1221:1254	coupling soft hydrogel 3D printing with lyophilization	1221:1274	In conclusion, we have developed a controlled hierarchical bone-like structure, biomimicking natural bone, together with a reproducible process of manufacture by coupling soft hydrogel 3D printing with lyophilization.
33761468	1	41	theme	multiscale	182:191	arg1	porosity					193:200	multiscale porosity	182:200	multiscale porosity	182:200	The aim of this work was to develop a complex-shaped gelatin-gellan composite scaffold with multiscale porosity using a combination of cryogenic 3D printing and lyophilization for bone tissue engineering.
33761468	7	42	theme	complex-shaped	1309:1322	arg1	scaffolds					1361:1369	complex-shaped patient-specific 3D printed hydrogel scaffolds	1309:1369	complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine	1309:1497	This enables the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine.
33761468	0	43	theme	3D	25:26	arg1	gellan-gelatin					36:49	a 3D printed gellan-gelatin	23:49	a 3D printed gellan-gelatin composite for bone tissue engineering	23:87	Multiscale porosity in a 3D printed gellan-gelatin composite for bone tissue engineering.
33761468	4	44	theme	vitrobiological	809:823	arg1	properties					825:834	their physical andin vitrobiological properties	788:834	their physical andin vitrobiological properties	788:834	These multiscale porous scaffolds (GMu) were compared with two other types of scaffolds having only microporosity (GMi) and macroporosity (GMa) with regard to their physical andin vitrobiological properties.
33761468	2	45	theme	pore	432:435	arg1	size					437:440	a pore size	430:440	a pore size of 919 ± 89 µm	430:455	Cryogenic 3D printing was used to fabricate a low-concentration composite of complex-shaped macroporous gelatin-gellan structures with a pore size of 919 ± 89 µm.
33761468	0	46	theme	composite	51:59	arg1	gellan-gelatin					36:49	a 3D printed gellan-gelatin	23:49	a 3D printed gellan-gelatin composite for bone tissue engineering	23:87	Multiscale porosity in a 3D printed gellan-gelatin composite for bone tissue engineering.
33761468	5	47	theme	GMu	837:839	arg1	scaffolds					841:849	GMu scaffolds	837:849	GMu scaffolds	837:849	GMu scaffolds were found to be better than GMi and GMa in terms of swelling percentage, degradation rate, uniform pore distribution, cellular infiltration, attachment, proliferation, protein generation and mineralization.
33761468	3	48	theme	hierarchical	598:609	arg1	structure					618:626	a hierarchical porous structure	596:626	a hierarchical porous structure	596:626	This was followed by lyophilization to introduce micropores of size 20-250 µm and nanometre-level surface functionalities, thus achieving a hierarchical porous structure.
33761468	2	49	theme	low-concentration	341:357	arg1	structures					414:423	complex-shaped macroporous gelatin-gellan structures	372:423	complex-shaped macroporous gelatin-gellan structures	372:423	Cryogenic 3D printing was used to fabricate a low-concentration composite of complex-shaped macroporous gelatin-gellan structures with a pore size of 919 ± 89 µm.
33761468	2	49	theme	low-concentration	341:357	arg1	composite					359:367	a low-concentration composite	339:367	a low-concentration composite of complex-shaped macroporous gelatin-gellan structures	339:423	Cryogenic 3D printing was used to fabricate a low-concentration composite of complex-shaped macroporous gelatin-gellan structures with a pore size of 919 ± 89 µm.
33761468	6	50	with	printing	1247:1254	arg1	lyophilization					1261:1274	lyophilization	1261:1274	lyophilization	1261:1274	In conclusion, we have developed a controlled hierarchical bone-like structure, biomimicking natural bone, together with a reproducible process of manufacture by coupling soft hydrogel 3D printing with lyophilization.
33761468	7	51	theme	scaffolds	1361:1369	arg1	development					1294:1304	the development	1290:1304	the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine	1290:1497	This enables the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine.
33761468	3	52	theme	porous	611:616	arg1	structure					618:626	a hierarchical porous structure	596:626	a hierarchical porous structure	596:626	This was followed by lyophilization to introduce micropores of size 20-250 µm and nanometre-level surface functionalities, thus achieving a hierarchical porous structure.
33761468	3	53	theme	size	521:524	arg1	µm					533:534	size 20-250 µm	521:534	size 20-250 µm	521:534	This was followed by lyophilization to introduce micropores of size 20-250 µm and nanometre-level surface functionalities, thus achieving a hierarchical porous structure.
33761468	1	54	theme	work	106:109	arg1	aim					94:96	The aim	90:96	The aim of this work	90:109	The aim of this work was to develop a complex-shaped gelatin-gellan composite scaffold with multiscale porosity using a combination of cryogenic 3D printing and lyophilization for bone tissue engineering.
33761468	7	55	theme	vitroand	1399:1406	arg1	potential					1414:1422	enhanced performancein vitroand great potential	1376:1422	enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine	1376:1497	This enables the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine.
33761468	7	56	theme	regenerative	1477:1488	arg1	fields					1431:1436	the fields	1427:1436	the fields of tissue engineering	1427:1458	This enables the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine.
33761468	7	56	theme	regenerative	1477:1488	arg1	medicine					1490:1497	regenerative medicine	1477:1497	regenerative medicine	1477:1497	This enables the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine.
33761468	5	57	theme	swelling	904:911	arg1	percentage					913:922	swelling percentage	904:922	swelling percentage	904:922	GMu scaffolds were found to be better than GMi and GMa in terms of swelling percentage, degradation rate, uniform pore distribution, cellular infiltration, attachment, proliferation, protein generation and mineralization.
33761468	6	58	theme	coupling	1221:1228	arg1	printing					1247:1254	coupling soft hydrogel 3D printing	1221:1254	coupling soft hydrogel 3D printing with lyophilization	1221:1274	In conclusion, we have developed a controlled hierarchical bone-like structure, biomimicking natural bone, together with a reproducible process of manufacture by coupling soft hydrogel 3D printing with lyophilization.
33761468	6	59	theme	3D	1244:1245	arg1	printing					1247:1254	coupling soft hydrogel 3D printing	1221:1254	coupling soft hydrogel 3D printing with lyophilization	1221:1274	In conclusion, we have developed a controlled hierarchical bone-like structure, biomimicking natural bone, together with a reproducible process of manufacture by coupling soft hydrogel 3D printing with lyophilization.
33761468	5	60	theme	percentage	913:922	arg1	attachment					993:1002	attachment	993:1002	attachment	993:1002	GMu scaffolds were found to be better than GMi and GMa in terms of swelling percentage, degradation rate, uniform pore distribution, cellular infiltration, attachment, proliferation, protein generation and mineralization.
33761468	5	60	theme	percentage	913:922	arg1	mineralization					1043:1056	mineralization	1043:1056	mineralization	1043:1056	GMu scaffolds were found to be better than GMi and GMa in terms of swelling percentage, degradation rate, uniform pore distribution, cellular infiltration, attachment, proliferation, protein generation and mineralization.
33761468	5	60	theme	percentage	913:922	arg1	distribution					956:967	uniform pore distribution	943:967	uniform pore distribution	943:967	GMu scaffolds were found to be better than GMi and GMa in terms of swelling percentage, degradation rate, uniform pore distribution, cellular infiltration, attachment, proliferation, protein generation and mineralization.
33761468	5	60	theme	percentage	913:922	arg1	proliferation					1005:1017	proliferation	1005:1017	proliferation	1005:1017	GMu scaffolds were found to be better than GMi and GMa in terms of swelling percentage, degradation rate, uniform pore distribution, cellular infiltration, attachment, proliferation, protein generation and mineralization.
33761468	5	60	theme	percentage	913:922	arg1	terms					895:899	terms	895:899	terms of swelling percentage	895:922	GMu scaffolds were found to be better than GMi and GMa in terms of swelling percentage, degradation rate, uniform pore distribution, cellular infiltration, attachment, proliferation, protein generation and mineralization.
33761468	5	60	theme	percentage	913:922	arg1	rate					937:940	degradation rate	925:940	degradation rate	925:940	GMu scaffolds were found to be better than GMi and GMa in terms of swelling percentage, degradation rate, uniform pore distribution, cellular infiltration, attachment, proliferation, protein generation and mineralization.
33761468	5	60	theme	percentage	913:922	arg1	generation					1028:1037	protein generation	1020:1037	protein generation	1020:1037	GMu scaffolds were found to be better than GMi and GMa in terms of swelling percentage, degradation rate, uniform pore distribution, cellular infiltration, attachment, proliferation, protein generation and mineralization.
33761468	5	60	theme	percentage	913:922	arg1	infiltration					979:990	cellular infiltration	970:990	cellular infiltration	970:990	GMu scaffolds were found to be better than GMi and GMa in terms of swelling percentage, degradation rate, uniform pore distribution, cellular infiltration, attachment, proliferation, protein generation and mineralization.
33761468	6	61	theme	bone-like	1118:1126	arg1	structure					1128:1136	a controlled hierarchical bone-like structure	1092:1136	a controlled hierarchical bone-like structure	1092:1136	In conclusion, we have developed a controlled hierarchical bone-like structure, biomimicking natural bone, together with a reproducible process of manufacture by coupling soft hydrogel 3D printing with lyophilization.
33761468	4	62	theme	multiscale	635:644	arg1	GMu					664:666	GMu	664:666	GMu	664:666	These multiscale porous scaffolds (GMu) were compared with two other types of scaffolds having only microporosity (GMi) and macroporosity (GMa) with regard to their physical andin vitrobiological properties.
33761468	4	62	theme	multiscale	635:644	arg1	scaffolds					653:661	These multiscale porous scaffolds	629:661	These multiscale porous scaffolds (GMu)	629:667	These multiscale porous scaffolds (GMu) were compared with two other types of scaffolds having only microporosity (GMi) and macroporosity (GMa) with regard to their physical andin vitrobiological properties.
33761468	1	63	with	scaffold	168:175	arg1	porosity					193:200	multiscale porosity	182:200	multiscale porosity	182:200	The aim of this work was to develop a complex-shaped gelatin-gellan composite scaffold with multiscale porosity using a combination of cryogenic 3D printing and lyophilization for bone tissue engineering.
33761468	3	64	theme	surface	556:562	arg1	functionalities					564:578	nanometre-level surface functionalities	540:578	nanometre-level surface functionalities	540:578	This was followed by lyophilization to introduce micropores of size 20-250 µm and nanometre-level surface functionalities, thus achieving a hierarchical porous structure.
33761468	3	65	theme	µm	533:534	arg1	micropores					507:516	micropores	507:516	micropores of size 20-250 µm and nanometre-level surface functionalities	507:578	This was followed by lyophilization to introduce micropores of size 20-250 µm and nanometre-level surface functionalities, thus achieving a hierarchical porous structure.
33761468	7	66	theme	hydrogel	1352:1359	arg1	scaffolds					1361:1369	complex-shaped patient-specific 3D printed hydrogel scaffolds	1309:1369	complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine	1309:1497	This enables the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine.
33761468	1	67	theme	cryogenic	225:233	arg1	printing					238:245	cryogenic 3D printing	225:245	cryogenic 3D printing	225:245	The aim of this work was to develop a complex-shaped gelatin-gellan composite scaffold with multiscale porosity using a combination of cryogenic 3D printing and lyophilization for bone tissue engineering.
33761468	0	68	theme	bone	65:68	arg1	engineering					77:87	bone tissue engineering	65:87	bone tissue engineering	65:87	Multiscale porosity in a 3D printed gellan-gelatin composite for bone tissue engineering.
33761468	6	69	theme	controlled	1094:1103	arg1	structure					1128:1136	a controlled hierarchical bone-like structure	1092:1136	a controlled hierarchical bone-like structure	1092:1136	In conclusion, we have developed a controlled hierarchical bone-like structure, biomimicking natural bone, together with a reproducible process of manufacture by coupling soft hydrogel 3D printing with lyophilization.
33761468	4	70	theme	andin	803:807	arg1	properties					825:834	their physical andin vitrobiological properties	788:834	their physical andin vitrobiological properties	788:834	These multiscale porous scaffolds (GMu) were compared with two other types of scaffolds having only microporosity (GMi) and macroporosity (GMa) with regard to their physical andin vitrobiological properties.
33761468	7	71	theme	3D	1341:1342	arg1	scaffolds					1361:1369	complex-shaped patient-specific 3D printed hydrogel scaffolds	1309:1369	complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine	1309:1497	This enables the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine.
33761468	2	72	theme	macroporous	387:397	arg1	structures					414:423	complex-shaped macroporous gelatin-gellan structures	372:423	complex-shaped macroporous gelatin-gellan structures	372:423	Cryogenic 3D printing was used to fabricate a low-concentration composite of complex-shaped macroporous gelatin-gellan structures with a pore size of 919 ± 89 µm.
33761468	2	73	used	used	321:324	arg2	printing					308:315	Cryogenic 3D printing	295:315	Cryogenic 3D printing	295:315	Cryogenic 3D printing was used to fabricate a low-concentration composite of complex-shaped macroporous gelatin-gellan structures with a pore size of 919 ± 89 µm.
33761468	1	74	theme	3D	235:236	arg1	printing					238:245	cryogenic 3D printing	225:245	cryogenic 3D printing	225:245	The aim of this work was to develop a complex-shaped gelatin-gellan composite scaffold with multiscale porosity using a combination of cryogenic 3D printing and lyophilization for bone tissue engineering.
33761468	6	75	theme	natural	1152:1158	arg1	bone					1160:1163	natural bone	1152:1163	natural bone	1152:1163	In conclusion, we have developed a controlled hierarchical bone-like structure, biomimicking natural bone, together with a reproducible process of manufacture by coupling soft hydrogel 3D printing with lyophilization.
33761468	4	76	theme	physical	794:801	arg1	properties					825:834	their physical andin vitrobiological properties	788:834	their physical andin vitrobiological properties	788:834	These multiscale porous scaffolds (GMu) were compared with two other types of scaffolds having only microporosity (GMi) and macroporosity (GMa) with regard to their physical andin vitrobiological properties.
33761468	2	77	theme	complex-shaped	372:385	arg1	structures					414:423	complex-shaped macroporous gelatin-gellan structures	372:423	complex-shaped macroporous gelatin-gellan structures	372:423	Cryogenic 3D printing was used to fabricate a low-concentration composite of complex-shaped macroporous gelatin-gellan structures with a pore size of 919 ± 89 µm.
33761468	7	78	theme	engineering	1448:1458	arg1	medicine					1490:1497	regenerative medicine	1477:1497	regenerative medicine	1477:1497	This enables the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine.
33761468	7	78	theme	engineering	1448:1458	arg1	bioprinting					1461:1471	bioprinting	1461:1471	bioprinting	1461:1471	This enables the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine.
33761468	7	78	theme	engineering	1448:1458	arg1	fields					1431:1436	the fields	1427:1436	the fields of tissue engineering	1427:1458	This enables the development of complex-shaped patient-specific 3D printed hydrogel scaffolds with enhanced performancein vitroand great potential in the fields of tissue engineering, bioprinting and regenerative medicine.
33761468	1	79	theme	printing	238:245	arg1	lyophilization					251:264	lyophilization	251:264	lyophilization for bone tissue engineering	251:292	The aim of this work was to develop a complex-shaped gelatin-gellan composite scaffold with multiscale porosity using a combination of cryogenic 3D printing and lyophilization for bone tissue engineering.
33761468	1	79	theme	printing	238:245	arg1	combination					210:220	a combination	208:220	a combination of cryogenic 3D printing	208:245	The aim of this work was to develop a complex-shaped gelatin-gellan composite scaffold with multiscale porosity using a combination of cryogenic 3D printing and lyophilization for bone tissue engineering.
34339971	7	0	theme	targeting	1169:1177	arg1	ability					1179:1185	targeting ability	1169:1185	targeting ability	1169:1185	This novel composite can promote the targeting ability and utilization efficiency of pesticides, thus having a huge potential application prospect in agriculture.
34339971	1	1	from	time	209:212	arg1	surfaces					222:229	crop surfaces	217:229	crop surfaces	217:229	For minimizing volatilization and leaching of pesticides, and enhancing their residence time on crop surfaces, we synthesized and characterized a novel intelligent pesticide delivery system.
34339971	3	2	theme	protective	508:517	arg1	shell					519:523	a protective shell	506:523	a protective shell	506:523	Ca-alginate was used as a structural matrix of the system and a protective shell to hinder the pesticide burst release from PK, and endowed the system with unique pH-sensitive property for IMI release.
34339971	3	2	theme	protective	508:517	arg1	Ca-alginate					444:454	Ca-alginate	444:454	Ca-alginate	444:454	Ca-alginate was used as a structural matrix of the system and a protective shell to hinder the pesticide burst release from PK, and endowed the system with unique pH-sensitive property for IMI release.
34339971	1	3	theme	intelligent	273:283	arg1	system					304:309	a novel intelligent pesticide delivery system	265:309	a novel intelligent pesticide delivery system	265:309	For minimizing volatilization and leaching of pesticides, and enhancing their residence time on crop surfaces, we synthesized and characterized a novel intelligent pesticide delivery system.
34339971	0	4	theme	pesticide	102:110	arg1	release					112:118	controlled pesticide release	91:118	controlled pesticide release	91:118	Stimuli-responsive Ca-alginate-based photothermal system with enhanced foliar adhesion for controlled pesticide release.
34339971	5	5	theme	excellent	852:860	arg1	property					878:885	the excellent light-sensitive property	848:885	the excellent light-sensitive property of detonation nanodiamond (DND) and temperature-responsive performance of poly(N-isopropylacrylamide) (PNIPAm)	848:996	Moreover, by the excellent light-sensitive property of detonation nanodiamond (DND) and temperature-responsive performance of poly(N-isopropylacrylamide) (PNIPAm), the release of IMI from the functional system could be adjusted by sunlight.
34339971	5	6	theme	light-sensitive	862:876	arg1	property					878:885	the excellent light-sensitive property	848:885	the excellent light-sensitive property of detonation nanodiamond (DND) and temperature-responsive performance of poly(N-isopropylacrylamide) (PNIPAm)	848:996	Moreover, by the excellent light-sensitive property of detonation nanodiamond (DND) and temperature-responsive performance of poly(N-isopropylacrylamide) (PNIPAm), the release of IMI from the functional system could be adjusted by sunlight.
34339971	1	7	theme	pesticide	285:293	arg1	system					304:309	a novel intelligent pesticide delivery system	265:309	a novel intelligent pesticide delivery system	265:309	For minimizing volatilization and leaching of pesticides, and enhancing their residence time on crop surfaces, we synthesized and characterized a novel intelligent pesticide delivery system.
34339971	3	8	with	endowed	576:582	arg1	property					620:627	unique pH-sensitive property	600:627	unique pH-sensitive property for IMI release	600:643	Ca-alginate was used as a structural matrix of the system and a protective shell to hinder the pesticide burst release from PK, and endowed the system with unique pH-sensitive property for IMI release.
34339971	3	9	theme	burst	549:553	arg1	release					555:561	the pesticide burst release	535:561	the pesticide burst release from PK	535:569	Ca-alginate was used as a structural matrix of the system and a protective shell to hinder the pesticide burst release from PK, and endowed the system with unique pH-sensitive property for IMI release.
34339971	1	10	theme	delivery	295:302	arg1	system					304:309	a novel intelligent pesticide delivery system	265:309	a novel intelligent pesticide delivery system	265:309	For minimizing volatilization and leaching of pesticides, and enhancing their residence time on crop surfaces, we synthesized and characterized a novel intelligent pesticide delivery system.
34339971	5	11	theme	performance	946:956	arg1	property					878:885	the excellent light-sensitive property	848:885	the excellent light-sensitive property of detonation nanodiamond (DND) and temperature-responsive performance of poly(N-isopropylacrylamide) (PNIPAm)	848:996	Moreover, by the excellent light-sensitive property of detonation nanodiamond (DND) and temperature-responsive performance of poly(N-isopropylacrylamide) (PNIPAm), the release of IMI from the functional system could be adjusted by sunlight.
34339971	3	12	theme	system	495:500	arg1	shell					519:523	a protective shell	506:523	a protective shell	506:523	Ca-alginate was used as a structural matrix of the system and a protective shell to hinder the pesticide burst release from PK, and endowed the system with unique pH-sensitive property for IMI release.
34339971	3	12	theme	system	495:500	arg1	Ca-alginate					444:454	Ca-alginate	444:454	Ca-alginate	444:454	Ca-alginate was used as a structural matrix of the system and a protective shell to hinder the pesticide burst release from PK, and endowed the system with unique pH-sensitive property for IMI release.
34339971	3	12	theme	system	495:500	arg1	matrix					481:486	a structural matrix	468:486	a structural matrix of the system	468:500	Ca-alginate was used as a structural matrix of the system and a protective shell to hinder the pesticide burst release from PK, and endowed the system with unique pH-sensitive property for IMI release.
34339971	4	13	theme	composite	809:817	arg1	adhesion					797:804	the adhesion	793:804	the adhesion of composite on crop leaves	793:832	The amino silicone oil (ASO) coating could bind with the waxy layer of crop leaves by the theory of "similarity-intermiscibility", which increased the adhesion of composite on crop leaves.
34339971	1	14	theme	residence	199:207	arg1	time					209:212	their residence time	193:212	their residence time on crop surfaces	193:229	For minimizing volatilization and leaching of pesticides, and enhancing their residence time on crop surfaces, we synthesized and characterized a novel intelligent pesticide delivery system.
34339971	7	15	theme	novel	1137:1141	arg1	composite					1143:1151	This novel composite	1132:1151	This novel composite	1132:1151	This novel composite can promote the targeting ability and utilization efficiency of pesticides, thus having a huge potential application prospect in agriculture.
34339971	3	16	used	used	460:463	arg2	Ca-alginate					444:454	Ca-alginate	444:454	Ca-alginate	444:454	Ca-alginate was used as a structural matrix of the system and a protective shell to hinder the pesticide burst release from PK, and endowed the system with unique pH-sensitive property for IMI release.
34339971	3	16	used	used	460:463	arg2	shell					519:523	a protective shell	506:523	a protective shell	506:523	Ca-alginate was used as a structural matrix of the system and a protective shell to hinder the pesticide burst release from PK, and endowed the system with unique pH-sensitive property for IMI release.
34339971	3	16	used	used	460:463	arg2	matrix					481:486	a structural matrix	468:486	a structural matrix of the system	468:500	Ca-alginate was used as a structural matrix of the system and a protective shell to hinder the pesticide burst release from PK, and endowed the system with unique pH-sensitive property for IMI release.
34339971	2	17	theme	adsorption	400:409	arg1	property					411:418	high adsorption property	395:418	high adsorption property through hydrogen bonds	395:441	Therein, imidacloprid (IMI) was adsorbed by polydopamine modified kaolin (PK) with high adsorption property through hydrogen bonds.
34339971	0	18	theme	Ca-alginate-based	19:35	arg1	system					50:55	Stimuli-responsive Ca-alginate-based photothermal system	0:55	Stimuli-responsive Ca-alginate-based photothermal system with enhanced foliar adhesion for controlled pesticide release.	0:119	Stimuli-responsive Ca-alginate-based photothermal system with enhanced foliar adhesion for controlled pesticide release.
34339971	7	19	theme	potential	1248:1256	arg1	prospect					1270:1277	a huge potential application prospect	1241:1277	a huge potential application prospect	1241:1277	This novel composite can promote the targeting ability and utilization efficiency of pesticides, thus having a huge potential application prospect in agriculture.
34339971	6	20	theme	control	1114:1120	arg1	efficacy					1122:1129	high control efficacy	1109:1129	high control efficacy	1109:1129	Besides, the composite displayed high control efficacy.
34339971	2	21	theme	high	395:398	arg1	property					411:418	high adsorption property	395:418	high adsorption property through hydrogen bonds	395:441	Therein, imidacloprid (IMI) was adsorbed by polydopamine modified kaolin (PK) with high adsorption property through hydrogen bonds.
34339971	0	22	theme	Stimuli-responsive	0:17	arg1	system					50:55	Stimuli-responsive Ca-alginate-based photothermal system	0:55	Stimuli-responsive Ca-alginate-based photothermal system with enhanced foliar adhesion for controlled pesticide release.	0:119	Stimuli-responsive Ca-alginate-based photothermal system with enhanced foliar adhesion for controlled pesticide release.
34339971	5	23	theme	poly	961:964	arg1	performance					946:956	temperature-responsive performance	923:956	temperature-responsive performance of poly(N-isopropylacrylamide) (PNIPAm)	923:996	Moreover, by the excellent light-sensitive property of detonation nanodiamond (DND) and temperature-responsive performance of poly(N-isopropylacrylamide) (PNIPAm), the release of IMI from the functional system could be adjusted by sunlight.
34339971	5	23	theme	poly	961:964	arg1	DND					914:916	DND	914:916	DND	914:916	Moreover, by the excellent light-sensitive property of detonation nanodiamond (DND) and temperature-responsive performance of poly(N-isopropylacrylamide) (PNIPAm), the release of IMI from the functional system could be adjusted by sunlight.
34339971	5	23	theme	poly	961:964	arg1	nanodiamond					901:911	detonation nanodiamond	890:911	detonation nanodiamond (DND)	890:917	Moreover, by the excellent light-sensitive property of detonation nanodiamond (DND) and temperature-responsive performance of poly(N-isopropylacrylamide) (PNIPAm), the release of IMI from the functional system could be adjusted by sunlight.
34339971	6	24	theme	high	1109:1112	arg1	efficacy					1122:1129	high control efficacy	1109:1129	high control efficacy	1109:1129	Besides, the composite displayed high control efficacy.
34339971	1	25	theme	crop	217:220	arg1	surfaces					222:229	crop surfaces	217:229	crop surfaces	217:229	For minimizing volatilization and leaching of pesticides, and enhancing their residence time on crop surfaces, we synthesized and characterized a novel intelligent pesticide delivery system.
34339971	5	26	theme	nanodiamond	901:911	arg1	property					878:885	the excellent light-sensitive property	848:885	the excellent light-sensitive property of detonation nanodiamond (DND) and temperature-responsive performance of poly(N-isopropylacrylamide) (PNIPAm)	848:996	Moreover, by the excellent light-sensitive property of detonation nanodiamond (DND) and temperature-responsive performance of poly(N-isopropylacrylamide) (PNIPAm), the release of IMI from the functional system could be adjusted by sunlight.
34339971	5	27	theme	temperature-responsive	923:944	arg1	performance					946:956	temperature-responsive performance	923:956	temperature-responsive performance of poly(N-isopropylacrylamide) (PNIPAm)	923:996	Moreover, by the excellent light-sensitive property of detonation nanodiamond (DND) and temperature-responsive performance of poly(N-isopropylacrylamide) (PNIPAm), the release of IMI from the functional system could be adjusted by sunlight.
34339971	0	28	theme	photothermal	37:48	arg1	system					50:55	Stimuli-responsive Ca-alginate-based photothermal system	0:55	Stimuli-responsive Ca-alginate-based photothermal system with enhanced foliar adhesion for controlled pesticide release.	0:119	Stimuli-responsive Ca-alginate-based photothermal system with enhanced foliar adhesion for controlled pesticide release.
34339971	7	29	dep	ability	1179:1185	arg1	the					1165:1167	the	1165:1167	the	1165:1167	This novel composite can promote the targeting ability and utilization efficiency of pesticides, thus having a huge potential application prospect in agriculture.
34339971	4	30	theme	crop	717:720	arg1	layer					708:712	the waxy layer	699:712	the waxy layer of crop leaves	699:727	The amino silicone oil (ASO) coating could bind with the waxy layer of crop leaves by the theory of "similarity-intermiscibility", which increased the adhesion of composite on crop leaves.
34339971	0	31	theme	enhanced	62:69	arg1	adhesion					78:85	enhanced foliar adhesion	62:85	enhanced foliar adhesion	62:85	Stimuli-responsive Ca-alginate-based photothermal system with enhanced foliar adhesion for controlled pesticide release.
34339971	4	32	dep	crop	717:720	arg1	leaves					722:727	leaves	722:727	leaves	722:727	The amino silicone oil (ASO) coating could bind with the waxy layer of crop leaves by the theory of "similarity-intermiscibility", which increased the adhesion of composite on crop leaves.
34339971	5	33	theme	functional	1027:1036	arg1	system					1038:1043	the functional system	1023:1043	the functional system	1023:1043	Moreover, by the excellent light-sensitive property of detonation nanodiamond (DND) and temperature-responsive performance of poly(N-isopropylacrylamide) (PNIPAm), the release of IMI from the functional system could be adjusted by sunlight.
34339971	4	34	theme	oil	665:667	arg1	coating					675:681	The amino silicone oil (ASO) coating	646:681	The amino silicone oil (ASO) coating	646:681	The amino silicone oil (ASO) coating could bind with the waxy layer of crop leaves by the theory of "similarity-intermiscibility", which increased the adhesion of composite on crop leaves.
34339971	2	35	theme	hydrogen	428:435	arg1	bonds					437:441	hydrogen bonds	428:441	hydrogen bonds	428:441	Therein, imidacloprid (IMI) was adsorbed by polydopamine modified kaolin (PK) with high adsorption property through hydrogen bonds.
34339971	4	36	theme	silicone	656:663	arg1	ASO					670:672	ASO	670:672	ASO	670:672	The amino silicone oil (ASO) coating could bind with the waxy layer of crop leaves by the theory of "similarity-intermiscibility", which increased the adhesion of composite on crop leaves.
34339971	4	36	theme	silicone	656:663	arg1	oil					665:667	amino silicone oil	650:667	The amino silicone oil (ASO) coating	646:681	The amino silicone oil (ASO) coating could bind with the waxy layer of crop leaves by the theory of "similarity-intermiscibility", which increased the adhesion of composite on crop leaves.
34339971	3	37	theme	IMI	633:635	arg1	release					637:643	IMI release	633:643	IMI release	633:643	Ca-alginate was used as a structural matrix of the system and a protective shell to hinder the pesticide burst release from PK, and endowed the system with unique pH-sensitive property for IMI release.
34339971	0	38	with	system	50:55	arg1	adhesion					78:85	enhanced foliar adhesion	62:85	enhanced foliar adhesion	62:85	Stimuli-responsive Ca-alginate-based photothermal system with enhanced foliar adhesion for controlled pesticide release.
34339971	3	39	theme	structural	470:479	arg1	Ca-alginate					444:454	Ca-alginate	444:454	Ca-alginate	444:454	Ca-alginate was used as a structural matrix of the system and a protective shell to hinder the pesticide burst release from PK, and endowed the system with unique pH-sensitive property for IMI release.
34339971	3	39	theme	structural	470:479	arg1	matrix					481:486	a structural matrix	468:486	a structural matrix of the system	468:500	Ca-alginate was used as a structural matrix of the system and a protective shell to hinder the pesticide burst release from PK, and endowed the system with unique pH-sensitive property for IMI release.
34339971	5	40	from	system	1038:1043	arg1	release					1003:1009	the release	999:1009	the release of IMI from the functional system	999:1043	Moreover, by the excellent light-sensitive property of detonation nanodiamond (DND) and temperature-responsive performance of poly(N-isopropylacrylamide) (PNIPAm), the release of IMI from the functional system could be adjusted by sunlight.
34339971	2	41	theme	modified	369:376	arg1	PK					386:387	PK	386:387	PK	386:387	Therein, imidacloprid (IMI) was adsorbed by polydopamine modified kaolin (PK) with high adsorption property through hydrogen bonds.
34339971	2	41	theme	modified	369:376	arg1	kaolin					378:383	polydopamine modified kaolin	356:383	polydopamine modified kaolin (PK) with high adsorption property through hydrogen bonds	356:441	Therein, imidacloprid (IMI) was adsorbed by polydopamine modified kaolin (PK) with high adsorption property through hydrogen bonds.
34339971	0	42	theme	foliar	71:76	arg1	adhesion					78:85	enhanced foliar adhesion	62:85	enhanced foliar adhesion	62:85	Stimuli-responsive Ca-alginate-based photothermal system with enhanced foliar adhesion for controlled pesticide release.
34339971	2	43	theme	polydopamine	356:367	arg1	PK					386:387	PK	386:387	PK	386:387	Therein, imidacloprid (IMI) was adsorbed by polydopamine modified kaolin (PK) with high adsorption property through hydrogen bonds.
34339971	2	43	theme	polydopamine	356:367	arg1	kaolin					378:383	polydopamine modified kaolin	356:383	polydopamine modified kaolin (PK) with high adsorption property through hydrogen bonds	356:441	Therein, imidacloprid (IMI) was adsorbed by polydopamine modified kaolin (PK) with high adsorption property through hydrogen bonds.
34339971	4	44	theme	"	774:774	arg1	theory					736:741	the theory	732:741	the theory of "similarity-intermiscibility", which increased the adhesion of composite on crop leaves	732:832	The amino silicone oil (ASO) coating could bind with the waxy layer of crop leaves by the theory of "similarity-intermiscibility", which increased the adhesion of composite on crop leaves.
34339971	2	45	with	kaolin	378:383	arg1	property					411:418	high adsorption property	395:418	high adsorption property through hydrogen bonds	395:441	Therein, imidacloprid (IMI) was adsorbed by polydopamine modified kaolin (PK) with high adsorption property through hydrogen bonds.
34339971	4	46	theme	similarity-intermiscibility	747:773	arg1	"					774:774	"similarity-intermiscibility"	746:774	"similarity-intermiscibility"	746:774	The amino silicone oil (ASO) coating could bind with the waxy layer of crop leaves by the theory of "similarity-intermiscibility", which increased the adhesion of composite on crop leaves.
34339971	4	47	theme	waxy	703:706	arg1	layer					708:712	the waxy layer	699:712	the waxy layer of crop leaves	699:727	The amino silicone oil (ASO) coating could bind with the waxy layer of crop leaves by the theory of "similarity-intermiscibility", which increased the adhesion of composite on crop leaves.
34339971	3	48	from	PK	568:569	arg1	release					555:561	the pesticide burst release	535:561	the pesticide burst release from PK	535:569	Ca-alginate was used as a structural matrix of the system and a protective shell to hinder the pesticide burst release from PK, and endowed the system with unique pH-sensitive property for IMI release.
34339971	3	49	theme	pesticide	539:547	arg1	release					555:561	the pesticide burst release	535:561	the pesticide burst release from PK	535:569	Ca-alginate was used as a structural matrix of the system and a protective shell to hinder the pesticide burst release from PK, and endowed the system with unique pH-sensitive property for IMI release.
34339971	5	50	theme	IMI	1014:1016	arg1	release					1003:1009	the release	999:1009	the release of IMI from the functional system	999:1043	Moreover, by the excellent light-sensitive property of detonation nanodiamond (DND) and temperature-responsive performance of poly(N-isopropylacrylamide) (PNIPAm), the release of IMI from the functional system could be adjusted by sunlight.
34339971	4	51	from	adhesion	797:804	arg1	crop					822:825	crop leaves	822:832	crop leaves	822:832	The amino silicone oil (ASO) coating could bind with the waxy layer of crop leaves by the theory of "similarity-intermiscibility", which increased the adhesion of composite on crop leaves.
34339971	1	52	theme	pesticides	167:176	arg1	leaching					155:162	leaching	155:162	leaching	155:162	For minimizing volatilization and leaching of pesticides, and enhancing their residence time on crop surfaces, we synthesized and characterized a novel intelligent pesticide delivery system.
34339971	1	52	theme	pesticides	167:176	arg1	volatilization					136:149	volatilization	136:149	volatilization	136:149	For minimizing volatilization and leaching of pesticides, and enhancing their residence time on crop surfaces, we synthesized and characterized a novel intelligent pesticide delivery system.
34339971	5	53	theme	detonation	890:899	arg1	DND					914:916	DND	914:916	DND	914:916	Moreover, by the excellent light-sensitive property of detonation nanodiamond (DND) and temperature-responsive performance of poly(N-isopropylacrylamide) (PNIPAm), the release of IMI from the functional system could be adjusted by sunlight.
34339971	5	53	theme	detonation	890:899	arg1	nanodiamond					901:911	detonation nanodiamond	890:911	detonation nanodiamond (DND)	890:917	Moreover, by the excellent light-sensitive property of detonation nanodiamond (DND) and temperature-responsive performance of poly(N-isopropylacrylamide) (PNIPAm), the release of IMI from the functional system could be adjusted by sunlight.
34339971	0	54	theme	controlled	91:100	arg1	release					112:118	controlled pesticide release	91:118	controlled pesticide release	91:118	Stimuli-responsive Ca-alginate-based photothermal system with enhanced foliar adhesion for controlled pesticide release.
34339971	7	55	theme	application	1258:1268	arg1	prospect					1270:1277	a huge potential application prospect	1241:1277	a huge potential application prospect	1241:1277	This novel composite can promote the targeting ability and utilization efficiency of pesticides, thus having a huge potential application prospect in agriculture.
34339971	3	56	theme	unique	600:605	arg1	property					620:627	unique pH-sensitive property	600:627	unique pH-sensitive property for IMI release	600:643	Ca-alginate was used as a structural matrix of the system and a protective shell to hinder the pesticide burst release from PK, and endowed the system with unique pH-sensitive property for IMI release.
34339971	7	57	theme	pesticides	1217:1226	arg1	ability					1179:1185	targeting ability	1169:1185	targeting ability	1169:1185	This novel composite can promote the targeting ability and utilization efficiency of pesticides, thus having a huge potential application prospect in agriculture.
34339971	7	57	theme	pesticides	1217:1226	arg1	efficiency					1203:1212	utilization efficiency	1191:1212	utilization efficiency	1191:1212	This novel composite can promote the targeting ability and utilization efficiency of pesticides, thus having a huge potential application prospect in agriculture.
34339971	4	58	theme	amino	650:654	arg1	ASO					670:672	ASO	670:672	ASO	670:672	The amino silicone oil (ASO) coating could bind with the waxy layer of crop leaves by the theory of "similarity-intermiscibility", which increased the adhesion of composite on crop leaves.
34339971	4	58	theme	amino	650:654	arg1	oil					665:667	amino silicone oil	650:667	The amino silicone oil (ASO) coating	646:681	The amino silicone oil (ASO) coating could bind with the waxy layer of crop leaves by the theory of "similarity-intermiscibility", which increased the adhesion of composite on crop leaves.
34339971	7	59	theme	utilization	1191:1201	arg1	efficiency					1203:1212	utilization efficiency	1191:1212	utilization efficiency	1191:1212	This novel composite can promote the targeting ability and utilization efficiency of pesticides, thus having a huge potential application prospect in agriculture.
34339971	3	60	theme	pH-sensitive	607:618	arg1	property					620:627	unique pH-sensitive property	600:627	unique pH-sensitive property for IMI release	600:643	Ca-alginate was used as a structural matrix of the system and a protective shell to hinder the pesticide burst release from PK, and endowed the system with unique pH-sensitive property for IMI release.
34339971	4	61	dep	crop	822:825	arg1	leaves					827:832	leaves	827:832	leaves	827:832	The amino silicone oil (ASO) coating could bind with the waxy layer of crop leaves by the theory of "similarity-intermiscibility", which increased the adhesion of composite on crop leaves.
34339971	7	62	theme	huge	1243:1246	arg1	prospect					1270:1277	a huge potential application prospect	1241:1277	a huge potential application prospect	1241:1277	This novel composite can promote the targeting ability and utilization efficiency of pesticides, thus having a huge potential application prospect in agriculture.
34339971	1	63	theme	novel	267:271	arg1	system					304:309	a novel intelligent pesticide delivery system	265:309	a novel intelligent pesticide delivery system	265:309	For minimizing volatilization and leaching of pesticides, and enhancing their residence time on crop surfaces, we synthesized and characterized a novel intelligent pesticide delivery system.
33171592	2	0	theme	acid	460:463	arg1	solutions					411:419	aqueous solutions	403:419	aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively	403:624	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	2	0	theme	acid	460:463	arg1	sources					496:502	Se sources	493:502	Se sources	493:502	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	6	1	theme	bactericidal	1320:1331	arg1	approaches					1333:1342	cytotoxic and bactericidal approaches	1306:1342	cytotoxic and bactericidal approaches	1306:1342	The biological activity of the Ag2Se NPs was assessed using cytotoxic and bactericidal approaches.
33171592	2	2	theme	dissolved	538:546	arg1	agents					605:610	reducing and stabilizing agents	580:610	agents	605:610	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	2	2	theme	dissolved	538:546	arg1	starch					561:566	starch	561:566	starch	561:566	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	2	2	theme	dissolved	538:546	arg1	fructose					548:555	fructose	548:555	fructose	548:555	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	2	3	theme	ultrasound-assisted	334:352	arg1	synthesis					354:362	the ultrasound-assisted synthesis	330:362	the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs)	330:391	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	5	4	theme	scanning	1175:1182	arg1	SEM-EDS					1236:1242	SEM-EDS	1236:1242	SEM-EDS	1236:1242	The optical properties of Ag2Se were studied using UV-Vis spectroscopy and its elemental composition was determined non-destructively using scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS).
33171592	5	4	theme	scanning	1175:1182	arg1	spectroscopy					1222:1233	scanning electron microscopy-energy-dispersive spectroscopy	1175:1233	scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS)	1175:1243	The optical properties of Ag2Se were studied using UV-Vis spectroscopy and its elemental composition was determined non-destructively using scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS).
33171592	2	5	theme	selenous	451:458	arg1	H2SeO3					466:471	H2SeO3	466:471	H2SeO3	466:471	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	2	5	theme	selenous	451:458	arg1	acid					460:463	selenous acid	451:463	selenous acid (H2SeO3)	451:472	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	5	6	theme	electron	1184:1191	arg1	SEM-EDS					1236:1242	SEM-EDS	1236:1242	SEM-EDS	1236:1242	The optical properties of Ag2Se were studied using UV-Vis spectroscopy and its elemental composition was determined non-destructively using scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS).
33171592	5	6	theme	electron	1184:1191	arg1	spectroscopy					1222:1233	scanning electron microscopy-energy-dispersive spectroscopy	1175:1233	scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS)	1175:1243	The optical properties of Ag2Se were studied using UV-Vis spectroscopy and its elemental composition was determined non-destructively using scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS).
33171592	3	7	theme	as-synthesized	765:778	arg1	NPs					786:788	the as-synthesized Ag2Se NPs	761:788	the as-synthesized Ag2Se NPs which, in turn, impact the optical properties of these NPs	761:847	The concentrations of mono- and polysaccharides were screened to determine their effect on the size, shape and colloidal stability of the as-synthesized Ag2Se NPs which, in turn, impact the optical properties of these NPs.
33171592	6	8	theme	cytotoxic	1306:1314	arg1	approaches					1333:1342	cytotoxic and bactericidal approaches	1306:1342	cytotoxic and bactericidal approaches	1306:1342	The biological activity of the Ag2Se NPs was assessed using cytotoxic and bactericidal approaches.
33171592	2	9	theme	reducing	580:587	arg1	agents					605:610	reducing and stabilizing agents	580:610	agents	605:610	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	2	9	theme	reducing	580:587	arg1	starch					561:566	starch	561:566	starch	561:566	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	2	9	theme	reducing	580:587	arg1	fructose					548:555	fructose	548:555	fructose	548:555	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	2	10	theme	first	289:293	arg1	time					295:298	the first time	285:298	the first time	285:298	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	5	11	theme	microscopy-energy-dispersive	1193:1220	arg1	SEM-EDS					1236:1242	SEM-EDS	1236:1242	SEM-EDS	1236:1242	The optical properties of Ag2Se were studied using UV-Vis spectroscopy and its elemental composition was determined non-destructively using scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS).
33171592	5	11	theme	microscopy-energy-dispersive	1193:1220	arg1	spectroscopy					1222:1233	scanning electron microscopy-energy-dispersive spectroscopy	1175:1233	scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS)	1175:1243	The optical properties of Ag2Se were studied using UV-Vis spectroscopy and its elemental composition was determined non-destructively using scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS).
33171592	2	12	theme	simple	303:308	arg1	method					319:324	a simple and easy method	301:324	a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs)	301:391	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	3	13	theme	polysaccharides	659:673	arg1	concentrations					631:644	The concentrations	627:644	The concentrations of mono- and polysaccharides	627:673	The concentrations of mono- and polysaccharides were screened to determine their effect on the size, shape and colloidal stability of the as-synthesized Ag2Se NPs which, in turn, impact the optical properties of these NPs.
33171592	7	14	theme	cost-effective	1378:1391	arg1	production					1412:1421	the cost-effective, fast and scalable production	1374:1421	the cost-effective, fast and scalable production of valuable Ag2Se NPs that may be utilized in numerous fields	1374:1483	Our findings pave the way to the cost-effective, fast and scalable production of valuable Ag2Se NPs that may be utilized in numerous fields.
33171592	5	15	theme	elemental	1114:1122	arg1	composition					1124:1134	its elemental composition	1110:1134	its elemental composition	1110:1134	The optical properties of Ag2Se were studied using UV-Vis spectroscopy and its elemental composition was determined non-destructively using scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS).
33171592	4	16	theme	as-synthesized	872:885	arg1	NPs					893:895	the as-synthesized Ag2Se NPs	868:895	the as-synthesized Ag2Se NPs	868:895	The morphology of the as-synthesized Ag2Se NPs was characterized by transmission electron microscopy (TEM) and both α- and β-phases of Ag2Se were determined by X-ray diffraction (XRD).
33171592	7	17	theme	scalable	1403:1410	arg1	production					1412:1421	the cost-effective, fast and scalable production	1374:1421	the cost-effective, fast and scalable production of valuable Ag2Se NPs that may be utilized in numerous fields	1374:1483	Our findings pave the way to the cost-effective, fast and scalable production of valuable Ag2Se NPs that may be utilized in numerous fields.
33171592	3	18	theme	colloidal	738:746	arg1	stability					748:756	colloidal stability	738:756	colloidal stability	738:756	The concentrations of mono- and polysaccharides were screened to determine their effect on the size, shape and colloidal stability of the as-synthesized Ag2Se NPs which, in turn, impact the optical properties of these NPs.
33171592	2	19	dep	report	273:278	arg1	To					240:241	To	240:241	To	240:241	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	1	20	theme	outstanding	170:180	arg1	properties					197:206	its outstanding optoelectronic properties	166:206	its outstanding optoelectronic properties	166:206	Silver selenide (Ag2Se) is a promising nanomaterial due to its outstanding optoelectronic properties and countless bio-applications.
33171592	4	21	theme	electron	931:938	arg1	TEM					952:954	TEM	952:954	TEM	952:954	The morphology of the as-synthesized Ag2Se NPs was characterized by transmission electron microscopy (TEM) and both α- and β-phases of Ag2Se were determined by X-ray diffraction (XRD).
33171592	4	21	theme	electron	931:938	arg1	microscopy					940:949	transmission electron microscopy	918:949	transmission electron microscopy (TEM)	918:955	The morphology of the as-synthesized Ag2Se NPs was characterized by transmission electron microscopy (TEM) and both α- and β-phases of Ag2Se were determined by X-ray diffraction (XRD).
33171592	3	22	theme	mono-	649:653	arg1	concentrations					631:644	The concentrations	627:644	The concentrations of mono- and polysaccharides	627:673	The concentrations of mono- and polysaccharides were screened to determine their effect on the size, shape and colloidal stability of the as-synthesized Ag2Se NPs which, in turn, impact the optical properties of these NPs.
33171592	2	23	theme	starch	561:566	arg1	presence					526:533	the presence	522:533	the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively	522:624	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	0	24	theme	Sugar-Mediated	0:13	arg1	Synthesis					21:29	Sugar-Mediated Green Synthesis	0:29	Sugar-Mediated Green Synthesis of Silver Selenide Semiconductor Nanocrystals under Ultrasound Irradiation.	0:105	Sugar-Mediated Green Synthesis of Silver Selenide Semiconductor Nanocrystals under Ultrasound Irradiation.
33171592	1	25	theme	optoelectronic	182:195	arg1	properties					197:206	its outstanding optoelectronic properties	166:206	its outstanding optoelectronic properties	166:206	Silver selenide (Ag2Se) is a promising nanomaterial due to its outstanding optoelectronic properties and countless bio-applications.
33171592	0	26	theme	Green	15:19	arg1	Synthesis					21:29	Sugar-Mediated Green Synthesis	0:29	Sugar-Mediated Green Synthesis of Silver Selenide Semiconductor Nanocrystals under Ultrasound Irradiation.	0:105	Sugar-Mediated Green Synthesis of Silver Selenide Semiconductor Nanocrystals under Ultrasound Irradiation.
33171592	7	27	theme	fast	1394:1397	arg1	production					1412:1421	the cost-effective, fast and scalable production	1374:1421	the cost-effective, fast and scalable production of valuable Ag2Se NPs that may be utilized in numerous fields	1374:1483	Our findings pave the way to the cost-effective, fast and scalable production of valuable Ag2Se NPs that may be utilized in numerous fields.
33171592	4	28	theme	NPs	893:895	arg1	morphology					854:863	The morphology	850:863	The morphology of the as-synthesized Ag2Se NPs	850:895	The morphology of the as-synthesized Ag2Se NPs was characterized by transmission electron microscopy (TEM) and both α- and β-phases of Ag2Se were determined by X-ray diffraction (XRD).
33171592	1	29	theme	Silver	107:112	arg1	selenide					114:121	Silver selenide	107:121	Silver selenide (Ag2Se)	107:129	Silver selenide (Ag2Se) is a promising nanomaterial due to its outstanding optoelectronic properties and countless bio-applications.
33171592	1	29	theme	Silver	107:112	arg1	nanomaterial					146:157	a promising nanomaterial	134:157	a promising nanomaterial due to its outstanding optoelectronic properties and countless bio-applications	134:237	Silver selenide (Ag2Se) is a promising nanomaterial due to its outstanding optoelectronic properties and countless bio-applications.
33171592	1	29	theme	Silver	107:112	arg1	Ag2Se					124:128	Ag2Se	124:128	Ag2Se	124:128	Silver selenide (Ag2Se) is a promising nanomaterial due to its outstanding optoelectronic properties and countless bio-applications.
33171592	3	30	theme	optical	817:823	arg1	properties					825:834	the optical properties	813:834	the optical properties of these NPs	813:847	The concentrations of mono- and polysaccharides were screened to determine their effect on the size, shape and colloidal stability of the as-synthesized Ag2Se NPs which, in turn, impact the optical properties of these NPs.
33171592	2	31	theme	nanoparticles	373:385	arg1	synthesis					354:362	the ultrasound-assisted synthesis	330:362	the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs)	330:391	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	2	32	theme	silver	424:429	arg1	AgNO3					440:444	AgNO3	440:444	AgNO3	440:444	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	2	32	theme	silver	424:429	arg1	nitrate					431:437	silver nitrate	424:437	silver nitrate (AgNO3)	424:445	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	2	33	theme	fructose	548:555	arg1	presence					526:533	the presence	522:533	the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively	522:624	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	7	34	theme	Ag2Se	1435:1439	arg1	NPs					1441:1443	valuable Ag2Se NPs	1426:1443	valuable Ag2Se NPs that may be utilized in numerous fields	1426:1483	Our findings pave the way to the cost-effective, fast and scalable production of valuable Ag2Se NPs that may be utilized in numerous fields.
33171592	4	35	theme	X-ray	1010:1014	arg1	diffraction					1016:1026	X-ray diffraction	1010:1026	X-ray diffraction (XRD)	1010:1032	The morphology of the as-synthesized Ag2Se NPs was characterized by transmission electron microscopy (TEM) and both α- and β-phases of Ag2Se were determined by X-ray diffraction (XRD).
33171592	4	35	theme	X-ray	1010:1014	arg1	XRD					1029:1031	XRD	1029:1031	XRD	1029:1031	The morphology of the as-synthesized Ag2Se NPs was characterized by transmission electron microscopy (TEM) and both α- and β-phases of Ag2Se were determined by X-ray diffraction (XRD).
33171592	2	36	theme	Ag2Se	367:371	arg1	NPs					388:390	NPs	388:390	NPs	388:390	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	2	36	theme	Ag2Se	367:371	arg1	nanoparticles					373:385	Ag2Se nanoparticles	367:385	Ag2Se nanoparticles (NPs)	367:391	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	7	37	used	utilized	1457:1464	arg2	NPs					1441:1443	valuable Ag2Se NPs	1426:1443	valuable Ag2Se NPs that may be utilized in numerous fields	1426:1483	Our findings pave the way to the cost-effective, fast and scalable production of valuable Ag2Se NPs that may be utilized in numerous fields.
33171592	6	38	theme	NPs	1283:1285	arg1	activity					1261:1268	The biological activity	1246:1268	The biological activity of the Ag2Se NPs	1246:1285	The biological activity of the Ag2Se NPs was assessed using cytotoxic and bactericidal approaches.
33171592	2	39	theme	stabilizing	593:603	arg1	agents					605:610	reducing and stabilizing agents	580:610	agents	605:610	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	2	39	theme	stabilizing	593:603	arg1	starch					561:566	starch	561:566	starch	561:566	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	2	39	theme	stabilizing	593:603	arg1	fructose					548:555	fructose	548:555	fructose	548:555	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	1	40	theme	countless	212:220	arg1	bio-applications					222:237	countless bio-applications	212:237	countless bio-applications	212:237	Silver selenide (Ag2Se) is a promising nanomaterial due to its outstanding optoelectronic properties and countless bio-applications.
33171592	2	41	theme	Se	493:494	arg1	solutions					411:419	aqueous solutions	403:419	aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively	403:624	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	2	41	theme	Se	493:494	arg1	sources					496:502	Se sources	493:502	Se sources	493:502	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	7	42	theme	numerous	1469:1476	arg1	fields					1478:1483	numerous fields	1469:1483	numerous fields	1469:1483	Our findings pave the way to the cost-effective, fast and scalable production of valuable Ag2Se NPs that may be utilized in numerous fields.
33171592	4	43	theme	transmission	918:929	arg1	TEM					952:954	TEM	952:954	TEM	952:954	The morphology of the as-synthesized Ag2Se NPs was characterized by transmission electron microscopy (TEM) and both α- and β-phases of Ag2Se were determined by X-ray diffraction (XRD).
33171592	4	43	theme	transmission	918:929	arg1	microscopy					940:949	transmission electron microscopy	918:949	transmission electron microscopy (TEM)	918:955	The morphology of the as-synthesized Ag2Se NPs was characterized by transmission electron microscopy (TEM) and both α- and β-phases of Ag2Se were determined by X-ray diffraction (XRD).
33171592	0	44	theme	Selenide	41:48	arg1	Nanocrystals					64:75	Silver Selenide Semiconductor Nanocrystals	34:75	Silver Selenide Semiconductor Nanocrystals	34:75	Sugar-Mediated Green Synthesis of Silver Selenide Semiconductor Nanocrystals under Ultrasound Irradiation.
33171592	5	45	theme	UV-Vis	1086:1091	arg1	spectroscopy					1093:1104	UV-Vis spectroscopy	1086:1104	UV-Vis spectroscopy	1086:1104	The optical properties of Ag2Se were studied using UV-Vis spectroscopy and its elemental composition was determined non-destructively using scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS).
33171592	3	46	theme	NPs	845:847	arg1	properties					825:834	the optical properties	813:834	the optical properties of these NPs	813:847	The concentrations of mono- and polysaccharides were screened to determine their effect on the size, shape and colloidal stability of the as-synthesized Ag2Se NPs which, in turn, impact the optical properties of these NPs.
33171592	0	47	theme	Silver	34:39	arg1	Nanocrystals					64:75	Silver Selenide Semiconductor Nanocrystals	34:75	Silver Selenide Semiconductor Nanocrystals	34:75	Sugar-Mediated Green Synthesis of Silver Selenide Semiconductor Nanocrystals under Ultrasound Irradiation.
33171592	5	48	theme	optical	1039:1045	arg1	properties					1047:1056	The optical properties	1035:1056	The optical properties of Ag2Se	1035:1065	The optical properties of Ag2Se were studied using UV-Vis spectroscopy and its elemental composition was determined non-destructively using scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS).
33171592	0	49	theme	Nanocrystals	64:75	arg1	Synthesis					21:29	Sugar-Mediated Green Synthesis	0:29	Sugar-Mediated Green Synthesis of Silver Selenide Semiconductor Nanocrystals under Ultrasound Irradiation.	0:105	Sugar-Mediated Green Synthesis of Silver Selenide Semiconductor Nanocrystals under Ultrasound Irradiation.
33171592	3	50	from	effect	708:713	arg1	shape					728:732	shape	728:732	shape	728:732	The concentrations of mono- and polysaccharides were screened to determine their effect on the size, shape and colloidal stability of the as-synthesized Ag2Se NPs which, in turn, impact the optical properties of these NPs.
33171592	3	50	from	effect	708:713	arg1	stability					748:756	colloidal stability	738:756	colloidal stability	738:756	The concentrations of mono- and polysaccharides were screened to determine their effect on the size, shape and colloidal stability of the as-synthesized Ag2Se NPs which, in turn, impact the optical properties of these NPs.
33171592	3	50	from	effect	708:713	arg1	size					722:725	size	722:725	size	722:725	The concentrations of mono- and polysaccharides were screened to determine their effect on the size, shape and colloidal stability of the as-synthesized Ag2Se NPs which, in turn, impact the optical properties of these NPs.
33171592	0	51	theme	Semiconductor	50:62	arg1	Nanocrystals					64:75	Silver Selenide Semiconductor Nanocrystals	34:75	Silver Selenide Semiconductor Nanocrystals	34:75	Sugar-Mediated Green Synthesis of Silver Selenide Semiconductor Nanocrystals under Ultrasound Irradiation.
33171592	6	52	theme	biological	1250:1259	arg1	activity					1261:1268	The biological activity	1246:1268	The biological activity of the Ag2Se NPs	1246:1285	The biological activity of the Ag2Se NPs was assessed using cytotoxic and bactericidal approaches.
33171592	1	53	theme	due	159:161	arg1	selenide					114:121	Silver selenide	107:121	Silver selenide (Ag2Se)	107:129	Silver selenide (Ag2Se) is a promising nanomaterial due to its outstanding optoelectronic properties and countless bio-applications.
33171592	1	53	theme	due	159:161	arg1	nanomaterial					146:157	a promising nanomaterial	134:157	a promising nanomaterial due to its outstanding optoelectronic properties and countless bio-applications	134:237	Silver selenide (Ag2Se) is a promising nanomaterial due to its outstanding optoelectronic properties and countless bio-applications.
33171592	4	54	theme	Ag2Se	985:989	arg1	α-					966:967	α-	966:967	α-	966:967	The morphology of the as-synthesized Ag2Se NPs was characterized by transmission electron microscopy (TEM) and both α- and β-phases of Ag2Se were determined by X-ray diffraction (XRD).
33171592	5	55	theme	Ag2Se	1061:1065	arg1	properties					1047:1056	The optical properties	1035:1056	The optical properties of Ag2Se	1035:1065	The optical properties of Ag2Se were studied using UV-Vis spectroscopy and its elemental composition was determined non-destructively using scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS).
33171592	2	56	theme	easy	314:317	arg1	method					319:324	a simple and easy method	301:324	a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs)	301:391	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	3	57	from	impact	806:811	arg1	turn					800:803	turn	800:803	turn	800:803	The concentrations of mono- and polysaccharides were screened to determine their effect on the size, shape and colloidal stability of the as-synthesized Ag2Se NPs which, in turn, impact the optical properties of these NPs.
33171592	7	58	theme	NPs	1441:1443	arg1	production					1412:1421	the cost-effective, fast and scalable production	1374:1421	the cost-effective, fast and scalable production of valuable Ag2Se NPs that may be utilized in numerous fields	1374:1483	Our findings pave the way to the cost-effective, fast and scalable production of valuable Ag2Se NPs that may be utilized in numerous fields.
33171592	6	59	theme	Ag2Se	1277:1281	arg1	NPs					1283:1285	the Ag2Se NPs	1273:1285	the Ag2Se NPs	1273:1285	The biological activity of the Ag2Se NPs was assessed using cytotoxic and bactericidal approaches.
33171592	1	60	theme	promising	136:144	arg1	selenide					114:121	Silver selenide	107:121	Silver selenide (Ag2Se)	107:129	Silver selenide (Ag2Se) is a promising nanomaterial due to its outstanding optoelectronic properties and countless bio-applications.
33171592	1	60	theme	promising	136:144	arg1	nanomaterial					146:157	a promising nanomaterial	134:157	a promising nanomaterial due to its outstanding optoelectronic properties and countless bio-applications	134:237	Silver selenide (Ag2Se) is a promising nanomaterial due to its outstanding optoelectronic properties and countless bio-applications.
33171592	3	61	theme	Ag2Se	780:784	arg1	NPs					786:788	the as-synthesized Ag2Se NPs	761:788	the as-synthesized Ag2Se NPs which, in turn, impact the optical properties of these NPs	761:847	The concentrations of mono- and polysaccharides were screened to determine their effect on the size, shape and colloidal stability of the as-synthesized Ag2Se NPs which, in turn, impact the optical properties of these NPs.
33171592	2	62	from	Ag	486:487	arg1	presence					526:533	the presence	522:533	the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively	522:624	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	0	63	theme	Ultrasound	83:92	arg1	Irradiation					94:104	Ultrasound Irradiation	83:104	Ultrasound Irradiation	83:104	Sugar-Mediated Green Synthesis of Silver Selenide Semiconductor Nanocrystals under Ultrasound Irradiation.
33171592	2	64	theme	aqueous	403:409	arg1	solutions					411:419	aqueous solutions	403:419	aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively	403:624	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	2	64	theme	aqueous	403:409	arg1	sources					496:502	Se sources	493:502	Se sources	493:502	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	3	65	theme	NPs	786:788	arg1	shape					728:732	shape	728:732	shape	728:732	The concentrations of mono- and polysaccharides were screened to determine their effect on the size, shape and colloidal stability of the as-synthesized Ag2Se NPs which, in turn, impact the optical properties of these NPs.
33171592	3	65	theme	NPs	786:788	arg1	stability					748:756	colloidal stability	738:756	colloidal stability	738:756	The concentrations of mono- and polysaccharides were screened to determine their effect on the size, shape and colloidal stability of the as-synthesized Ag2Se NPs which, in turn, impact the optical properties of these NPs.
33171592	3	65	theme	NPs	786:788	arg1	size					722:725	size	722:725	size	722:725	The concentrations of mono- and polysaccharides were screened to determine their effect on the size, shape and colloidal stability of the as-synthesized Ag2Se NPs which, in turn, impact the optical properties of these NPs.
33171592	2	66	from	sources	496:502	arg1	presence					526:533	the presence	522:533	the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively	522:624	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	4	67	theme	Ag2Se	887:891	arg1	NPs					893:895	the as-synthesized Ag2Se NPs	868:895	the as-synthesized Ag2Se NPs	868:895	The morphology of the as-synthesized Ag2Se NPs was characterized by transmission electron microscopy (TEM) and both α- and β-phases of Ag2Se were determined by X-ray diffraction (XRD).
33171592	7	68	theme	valuable	1426:1433	arg1	NPs					1441:1443	valuable Ag2Se NPs	1426:1443	valuable Ag2Se NPs that may be utilized in numerous fields	1426:1483	Our findings pave the way to the cost-effective, fast and scalable production of valuable Ag2Se NPs that may be utilized in numerous fields.
33171592	2	69	theme	nitrate	431:437	arg1	solutions					411:419	aqueous solutions	403:419	aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively	403:624	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
33171592	2	69	theme	nitrate	431:437	arg1	sources					496:502	Se sources	493:502	Se sources	493:502	To the best of our knowledge, we report, for the first time, a simple and easy method for the ultrasound-assisted synthesis of Ag2Se nanoparticles (NPs) by mixing aqueous solutions of silver nitrate (AgNO3) and selenous acid (H2SeO3) that act as Ag and Se sources, respectively, in the presence of dissolved fructose and starch that act as reducing and stabilizing agents, respectively.
34562533	5	0	theme	highest	736:742	arg1	roughness					760:768	the highest average surface roughness	732:768	the highest average surface roughness	732:768	The BC-Chi has the highest average surface roughness.
34562533	8	1	dep	in	1160:1161	arg1	vitro					1163:1167	vitro	1163:1167	vitro	1163:1167	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	7	2	with	potential	933:941	arg1	reduction					958:966	up to 90% reduction	948:966	up to 90% reduction of viable biofilm	948:984	New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height.
34562533	7	2	with	potential	933:941	arg1	reduction					1000:1008	up to 65% reduction	990:1008	up to 65% reduction of biofilm height	990:1026	New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height.
34562533	0	3	theme	cellulose-chitosan	84:101	arg1	polymer					103:109	bacterial cellulose-chitosan polymer	74:109	bacterial cellulose-chitosan polymer	74:109	Chronic wound dressings - Pathogenic bacteria anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels.
34562533	6	4	theme	irradiated	798:807	arg1	increase					824:831	an irradiated dose-dependent increase	795:831	an irradiated dose-dependent increase of average surface roughness	795:860	BC-nChiD hydrogels show an irradiated dose-dependent increase of average surface roughness.
34562533	4	5	theme	Chi	677:679	arg1	incorporation					660:672	incorporation	660:672	incorporation of Chi	660:679	Physical and chemical analyses showed incorporation of Chi and encapsulation of nChiD into BC.
34562533	4	5	theme	Chi	677:679	arg1	encapsulation					685:697	encapsulation	685:697	encapsulation of nChiD into BC	685:714	Physical and chemical analyses showed incorporation of Chi and encapsulation of nChiD into BC.
34562533	7	6	theme	composite	867:875	arg1	hydrogels					877:885	New composite hydrogels	863:885	New composite hydrogels	863:885	New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height.
34562533	0	7	theme	bacterial	114:122	arg1	dots					143:146	bacterial cellulose-chitosan dots	114:146	bacterial cellulose-chitosan dots	114:146	Chronic wound dressings - Pathogenic bacteria anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels.
34562533	5	8	contain	has	728:730	arg1	BC-Chi					721:726	The BC-Chi	717:726	The BC-Chi	717:726	The BC-Chi has the highest average surface roughness.
34562533	5	8	contain	has	728:730	arg2	roughness					760:768	the highest average surface roughness	732:768	the highest average surface roughness	732:768	The BC-Chi has the highest average surface roughness.
34562533	7	9	theme	viable	971:976	arg1	biofilm					978:984	viable biofilm	971:984	viable biofilm	971:984	New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height.
34562533	7	10	dep	90	954:955	arg1	to					951:952	to	951:952	to	951:952	New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height.
34562533	1	11	dep	bacteria	190:197	arg1	biofilms					199:206	biofilms	199:206	the pathogenic bacteria biofilms	175:206	Since the pathogenic bacteria biofilms are involved in 70% of chronic infections and their resistance to antibiotics is increased, the research in this field requires new healing agents.
34562533	8	12	dep	characteristics	1065:1079	arg1	absorption					1118:1127	higher wound fluid absorption	1099:1127	higher wound fluid absorption	1099:1127	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	8	12	dep	characteristics	1065:1079	arg1	migration					1140:1148	faster migration	1133:1148	faster migration of cells (in vitro healing)	1133:1176	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	8	12	dep	characteristics	1065:1079	arg1	characteristics					1065:1079	better dressing characteristics	1049:1079	better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing)	1049:1176	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	8	12	dep	characteristics	1065:1079	arg1	porosity					1089:1096	higher porosity	1082:1096	higher porosity	1082:1096	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	7	13	dep	65	996:997	arg1	to					993:994	to	993:994	to	993:994	New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height.
34562533	8	14	theme	in	1160:1161	arg1	cells					1153:1157	cells	1153:1157	cells (in vitro healing)	1153:1176	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	8	14	theme	in	1160:1161	arg1	healing					1169:1175	in vitro healing	1160:1175	in vitro healing	1160:1175	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	7	15	theme	New	863:865	arg1	hydrogels					877:885	New composite hydrogels	863:885	New composite hydrogels	863:885	New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height.
34562533	4	16	theme	chemical	635:642	arg1	analyses					644:651	Physical and chemical analyses	622:651	Physical and chemical analyses	622:651	Physical and chemical analyses showed incorporation of Chi and encapsulation of nChiD into BC.
34562533	0	17	theme	cellulose-chitosan	124:141	arg1	dots					143:146	bacterial cellulose-chitosan dots	114:146	bacterial cellulose-chitosan dots	114:146	Chronic wound dressings - Pathogenic bacteria anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels.
34562533	2	18	with	cellulose	453:461	arg1	nanoparticles					518:530	chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles	473:530	chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD)	473:538	New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD.
34562533	2	18	with	cellulose	453:461	arg1	nChiD					533:537	nChiD	533:537	nChiD	533:537	New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD.
34562533	9	19	theme	healing	1262:1268	arg1	hydrogels					1225:1233	both composite hydrogels	1210:1233	both composite hydrogels	1210:1233	All obtained results confirmed both composite hydrogels as promising chronic wound healing agents.
34562533	9	19	theme	healing	1262:1268	arg1	agents					1270:1275	promising chronic wound healing agents	1238:1275	promising chronic wound healing agents	1238:1275	All obtained results confirmed both composite hydrogels as promising chronic wound healing agents.
34562533	6	20	theme	BC-nChiD	771:778	arg1	hydrogels					780:788	BC-nChiD hydrogels	771:788	BC-nChiD hydrogels	771:788	BC-nChiD hydrogels show an irradiated dose-dependent increase of average surface roughness.
34562533	7	21	dep	reduction	1000:1008	arg1	%					998:998	%	998:998	%	998:998	New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height.
34562533	2	22	theme	wound	415:419	arg1	dressings					421:429	potential chronic wound dressings	397:429	potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD	397:549	New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD.
34562533	2	22	theme	wound	415:419	arg1	hydrogels					370:378	New composite hydrogels	356:378	New composite hydrogels	356:378	New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD.
34562533	0	23	theme	wound	8:12	arg1	dressings					14:22	Chronic wound dressings	0:22	Chronic wound dressings	0:22	Chronic wound dressings - Pathogenic bacteria anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels.
34562533	5	24	theme	average	744:750	arg1	roughness					760:768	the highest average surface roughness	732:768	the highest average surface roughness	732:768	The BC-Chi has the highest average surface roughness.
34562533	8	25	theme	dressing	1056:1063	arg1	characteristics					1065:1079	better dressing characteristics	1049:1079	better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing)	1049:1176	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	8	25	theme	dressing	1056:1063	arg1	migration					1140:1148	faster migration	1133:1148	faster migration of cells (in vitro healing)	1133:1176	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	8	25	theme	dressing	1056:1063	arg1	absorption					1118:1127	higher wound fluid absorption	1099:1127	higher wound fluid absorption	1099:1127	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	8	25	theme	dressing	1056:1063	arg1	porosity					1089:1096	higher porosity	1082:1096	higher porosity	1082:1096	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	8	26	theme	better	1049:1054	arg1	characteristics					1065:1079	better dressing characteristics	1049:1079	better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing)	1049:1176	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	8	26	theme	better	1049:1054	arg1	migration					1140:1148	faster migration	1133:1148	faster migration of cells (in vitro healing)	1133:1176	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	8	26	theme	better	1049:1054	arg1	absorption					1118:1127	higher wound fluid absorption	1099:1127	higher wound fluid absorption	1099:1127	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	8	26	theme	better	1049:1054	arg1	porosity					1089:1096	higher porosity	1082:1096	higher porosity	1082:1096	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	0	27	dep	polymer	103:109	arg1	hydrogels					158:166	composite hydrogels	148:166	composite hydrogels	148:166	Chronic wound dressings - Pathogenic bacteria anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels.
34562533	2	28	theme	chronic	407:413	arg1	dressings					421:429	potential chronic wound dressings	397:429	potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD	397:549	New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD.
34562533	2	28	theme	chronic	407:413	arg1	hydrogels					370:378	New composite hydrogels	356:378	New composite hydrogels	356:378	New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD.
34562533	0	29	theme	Chronic	0:6	arg1	dressings					14:22	Chronic wound dressings	0:22	Chronic wound dressings	0:22	Chronic wound dressings - Pathogenic bacteria anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels.
34562533	1	30	from	research	304:311	arg1	field					321:325	this field	316:325	this field	316:325	Since the pathogenic bacteria biofilms are involved in 70% of chronic infections and their resistance to antibiotics is increased, the research in this field requires new healing agents.
34562533	6	31	theme	average	836:842	arg1	roughness					852:860	average surface roughness	836:860	average surface roughness	836:860	BC-nChiD hydrogels show an irradiated dose-dependent increase of average surface roughness.
34562533	9	32	theme	obtained	1183:1190	arg1	results					1192:1198	All obtained results	1179:1198	All obtained results	1179:1198	All obtained results confirmed both composite hydrogels as promising chronic wound healing agents.
34562533	2	33	theme	potential	397:405	arg1	dressings					421:429	potential chronic wound dressings	397:429	potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD	397:549	New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD.
34562533	2	33	theme	potential	397:405	arg1	hydrogels					370:378	New composite hydrogels	356:378	New composite hydrogels	356:378	New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD.
34562533	9	34	theme	composite	1215:1223	arg1	hydrogels					1225:1233	both composite hydrogels	1210:1233	both composite hydrogels	1210:1233	All obtained results confirmed both composite hydrogels as promising chronic wound healing agents.
34562533	9	34	theme	composite	1215:1223	arg1	agents					1270:1275	promising chronic wound healing agents	1238:1275	promising chronic wound healing agents	1238:1275	All obtained results confirmed both composite hydrogels as promising chronic wound healing agents.
34562533	0	35	theme	composite	148:156	arg1	hydrogels					158:166	composite hydrogels	148:166	composite hydrogels	148:166	Chronic wound dressings - Pathogenic bacteria anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels.
34562533	8	36	theme	faster	1133:1138	arg1	characteristics					1065:1079	better dressing characteristics	1049:1079	better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing)	1049:1176	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	8	36	theme	faster	1133:1138	arg1	migration					1140:1148	faster migration	1133:1148	faster migration of cells (in vitro healing)	1133:1176	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	2	37	theme	bacterial	443:451	arg1	BC					464:465	BC	464:465	BC	464:465	New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD.
34562533	2	37	theme	bacterial	443:451	arg1	cellulose					453:461	bacterial cellulose	443:461	bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD)	443:538	New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD.
34562533	5	38	theme	surface	752:758	arg1	roughness					760:768	the highest average surface roughness	732:768	the highest average surface roughness	732:768	The BC-Chi has the highest average surface roughness.
34562533	7	39	dep	%	998:998	arg1	65					996:997	65	996:997	65	996:997	New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height.
34562533	4	40	theme	Physical	622:629	arg1	analyses					644:651	Physical and chemical analyses	622:651	Physical and chemical analyses	622:651	Physical and chemical analyses showed incorporation of Chi and encapsulation of nChiD into BC.
34562533	0	41	dep	treatment	59:67	arg1	dressings					14:22	Chronic wound dressings	0:22	Chronic wound dressings	0:22	Chronic wound dressings - Pathogenic bacteria anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels.
34562533	0	41	dep	treatment	59:67	arg1	bacteria					37:44	Pathogenic bacteria	26:44	Pathogenic bacteria	26:44	Chronic wound dressings - Pathogenic bacteria anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels.
34562533	3	42	dep	doses	596:600	arg1	20					603:604	20	603:604	20	603:604	nChiD were obtained by gamma irradiation at doses: 20, 40 and 60 kGy.
34562533	3	42	dep	doses	596:600	arg1	60 kGy					614:619	60 kGy	614:619	60 kGy	614:619	nChiD were obtained by gamma irradiation at doses: 20, 40 and 60 kGy.
34562533	3	42	dep	doses	596:600	arg1	40					607:608	40	607:608	40	607:608	nChiD were obtained by gamma irradiation at doses: 20, 40 and 60 kGy.
34562533	1	43	theme	chronic	231:237	arg1	infections					239:248	chronic infections	231:248	chronic infections	231:248	Since the pathogenic bacteria biofilms are involved in 70% of chronic infections and their resistance to antibiotics is increased, the research in this field requires new healing agents.
34562533	0	44	theme	Pathogenic	26:35	arg1	bacteria					37:44	Pathogenic bacteria	26:44	Pathogenic bacteria	26:44	Chronic wound dressings - Pathogenic bacteria anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels.
34562533	8	45	theme	higher	1082:1087	arg1	characteristics					1065:1079	better dressing characteristics	1049:1079	better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing)	1049:1176	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	8	45	theme	higher	1082:1087	arg1	porosity					1089:1096	higher porosity	1082:1096	higher porosity	1082:1096	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	6	46	theme	roughness	852:860	arg1	increase					824:831	an irradiated dose-dependent increase	795:831	an irradiated dose-dependent increase of average surface roughness	795:860	BC-nChiD hydrogels show an irradiated dose-dependent increase of average surface roughness.
34562533	1	47	theme	infections	239:248	arg1	%					226:226	70%	224:226	70% of chronic infections	224:248	Since the pathogenic bacteria biofilms are involved in 70% of chronic infections and their resistance to antibiotics is increased, the research in this field requires new healing agents.
34562533	1	47	theme	infections	239:248	arg1	infections					239:248	chronic infections	231:248	chronic infections	231:248	Since the pathogenic bacteria biofilms are involved in 70% of chronic infections and their resistance to antibiotics is increased, the research in this field requires new healing agents.
34562533	2	48	theme	polymer	482:488	arg1	nanoparticles					518:530	chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles	473:530	chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD)	473:538	New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD.
34562533	2	48	theme	polymer	482:488	arg1	nChiD					533:537	nChiD	533:537	nChiD	533:537	New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD.
34562533	8	49	theme	cells	1153:1157	arg1	migration					1140:1148	faster migration	1133:1148	faster migration of cells (in vitro healing)	1133:1176	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	8	49	theme	cells	1153:1157	arg1	absorption					1118:1127	higher wound fluid absorption	1099:1127	higher wound fluid absorption	1099:1127	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	8	49	theme	cells	1153:1157	arg1	porosity					1089:1096	higher porosity	1082:1096	higher porosity	1082:1096	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	8	49	theme	cells	1153:1157	arg1	characteristics					1065:1079	better dressing characteristics	1049:1079	better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing)	1049:1176	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	8	50	theme	higher	1099:1104	arg1	absorption					1118:1127	higher wound fluid absorption	1099:1127	higher wound fluid absorption	1099:1127	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	8	50	theme	higher	1099:1104	arg1	characteristics					1065:1079	better dressing characteristics	1049:1079	better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing)	1049:1176	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	6	51	theme	surface	844:850	arg1	roughness					852:860	average surface roughness	836:860	average surface roughness	836:860	BC-nChiD hydrogels show an irradiated dose-dependent increase of average surface roughness.
34562533	3	52	theme	gamma	575:579	arg1	irradiation					581:591	gamma irradiation	575:591	gamma irradiation	575:591	nChiD were obtained by gamma irradiation at doses: 20, 40 and 60 kGy.
34562533	8	53	theme	fluid	1112:1116	arg1	absorption					1118:1127	higher wound fluid absorption	1099:1127	higher wound fluid absorption	1099:1127	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	8	53	theme	fluid	1112:1116	arg1	characteristics					1065:1079	better dressing characteristics	1049:1079	better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing)	1049:1176	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	2	54	theme	composite	360:368	arg1	dressings					421:429	potential chronic wound dressings	397:429	potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD	397:549	New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD.
34562533	2	54	theme	composite	360:368	arg1	hydrogels					370:378	New composite hydrogels	356:378	New composite hydrogels	356:378	New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD.
34562533	0	55	theme	anti-biofilm	46:57	arg1	treatment					59:67	anti-biofilm treatment	46:67	anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels	46:166	Chronic wound dressings - Pathogenic bacteria anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels.
34562533	1	56	theme	new	336:338	arg1	agents					348:353	new healing agents	336:353	new healing agents	336:353	Since the pathogenic bacteria biofilms are involved in 70% of chronic infections and their resistance to antibiotics is increased, the research in this field requires new healing agents.
34562533	7	57	dep	%	956:956	arg1	90					954:955	90	954:955	90	954:955	New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height.
34562533	2	58	theme	chitosan	473:480	arg1	polymer					482:488	chitosan polymer	473:488	chitosan polymer (Chi)	473:494	New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD.
34562533	2	58	theme	chitosan	473:480	arg1	Chi					491:493	Chi	491:493	Chi	491:493	New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD.
34562533	2	59	theme	New	356:358	arg1	dressings					421:429	potential chronic wound dressings	397:429	potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD	397:549	New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD.
34562533	2	59	theme	New	356:358	arg1	hydrogels					370:378	New composite hydrogels	356:378	New composite hydrogels	356:378	New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD.
34562533	7	60	theme	anti-biofilm	920:931	arg1	potential					933:941	excellent anti-biofilm potential	910:941	excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height	910:1026	New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height.
34562533	1	61	theme	healing	340:346	arg1	agents					348:353	new healing agents	336:353	new healing agents	336:353	Since the pathogenic bacteria biofilms are involved in 70% of chronic infections and their resistance to antibiotics is increased, the research in this field requires new healing agents.
34562533	7	62	theme	biofilm	1013:1019	arg1	reduction					958:966	up to 90% reduction	948:966	up to 90% reduction of viable biofilm	948:984	New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height.
34562533	7	62	theme	biofilm	1013:1019	arg1	reduction					1000:1008	up to 65% reduction	990:1008	up to 65% reduction of biofilm height	990:1026	New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height.
34562533	9	63	theme	chronic	1248:1254	arg1	hydrogels					1225:1233	both composite hydrogels	1210:1233	both composite hydrogels	1210:1233	All obtained results confirmed both composite hydrogels as promising chronic wound healing agents.
34562533	9	63	theme	chronic	1248:1254	arg1	agents					1270:1275	promising chronic wound healing agents	1238:1275	promising chronic wound healing agents	1238:1275	All obtained results confirmed both composite hydrogels as promising chronic wound healing agents.
34562533	7	64	dep	reduction	958:966	arg1	%					956:956	%	956:956	%	956:956	New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height.
34562533	8	65	theme	wound	1106:1110	arg1	absorption					1118:1127	higher wound fluid absorption	1099:1127	higher wound fluid absorption	1099:1127	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	8	65	theme	wound	1106:1110	arg1	characteristics					1065:1079	better dressing characteristics	1049:1079	better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing)	1049:1176	The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing).
34562533	9	66	theme	wound	1256:1260	arg1	hydrogels					1225:1233	both composite hydrogels	1210:1233	both composite hydrogels	1210:1233	All obtained results confirmed both composite hydrogels as promising chronic wound healing agents.
34562533	9	66	theme	wound	1256:1260	arg1	agents					1270:1275	promising chronic wound healing agents	1238:1275	promising chronic wound healing agents	1238:1275	All obtained results confirmed both composite hydrogels as promising chronic wound healing agents.
34562533	0	67	with	treatment	59:67	arg1	polymer					103:109	bacterial cellulose-chitosan polymer	74:109	bacterial cellulose-chitosan polymer	74:109	Chronic wound dressings - Pathogenic bacteria anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels.
34562533	0	67	with	treatment	59:67	arg1	dots					143:146	bacterial cellulose-chitosan dots	114:146	bacterial cellulose-chitosan dots	114:146	Chronic wound dressings - Pathogenic bacteria anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels.
34562533	4	68	theme	nChiD	702:706	arg1	incorporation					660:672	incorporation	660:672	incorporation of Chi	660:679	Physical and chemical analyses showed incorporation of Chi and encapsulation of nChiD into BC.
34562533	4	68	theme	nChiD	702:706	arg1	encapsulation					685:697	encapsulation	685:697	encapsulation of nChiD into BC	685:714	Physical and chemical analyses showed incorporation of Chi and encapsulation of nChiD into BC.
34562533	0	69	theme	bacterial	74:82	arg1	polymer					103:109	bacterial cellulose-chitosan polymer	74:109	bacterial cellulose-chitosan polymer	74:109	Chronic wound dressings - Pathogenic bacteria anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels.
34562533	6	70	theme	dose-dependent	809:822	arg1	increase					824:831	an irradiated dose-dependent increase	795:831	an irradiated dose-dependent increase of average surface roughness	795:860	BC-nChiD hydrogels show an irradiated dose-dependent increase of average surface roughness.
34562533	9	71	theme	promising	1238:1246	arg1	hydrogels					1225:1233	both composite hydrogels	1210:1233	both composite hydrogels	1210:1233	All obtained results confirmed both composite hydrogels as promising chronic wound healing agents.
34562533	9	71	theme	promising	1238:1246	arg1	agents					1270:1275	promising chronic wound healing agents	1238:1275	promising chronic wound healing agents	1238:1275	All obtained results confirmed both composite hydrogels as promising chronic wound healing agents.
34562533	7	72	theme	excellent	910:918	arg1	potential					933:941	excellent anti-biofilm potential	910:941	excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height	910:1026	New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height.
34562533	7	73	with	biocompatible	891:903	arg1	potential					933:941	excellent anti-biofilm potential	910:941	excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height	910:1026	New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height.
34562533	2	74	theme	BC-Chi	498:503	arg1	nanoparticles					518:530	chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles	473:530	chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD)	473:538	New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD.
34562533	2	74	theme	BC-Chi	498:503	arg1	nChiD					533:537	nChiD	533:537	nChiD	533:537	New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) - BC-Chi and chitosan nanoparticles (nChiD) - BC-nChiD.
34562533	7	75	theme	biofilm	978:984	arg1	reduction					958:966	up to 90% reduction	948:966	up to 90% reduction of viable biofilm	948:984	New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height.
34562533	7	75	theme	biofilm	978:984	arg1	reduction					1000:1008	up to 65% reduction	990:1008	up to 65% reduction of biofilm height	990:1026	New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height.
34562533	1	76	theme	pathogenic	179:188	arg1	bacteria					190:197	the pathogenic bacteria	175:197	the pathogenic bacteria biofilms	175:206	Since the pathogenic bacteria biofilms are involved in 70% of chronic infections and their resistance to antibiotics is increased, the research in this field requires new healing agents.
34382058	12	0	theme	affected	1828:1835	arg1	levels					1860:1865	thus affected tight junction protein levels	1823:1865	thus affected tight junction protein levels	1823:1865	In contrast, the changes in metabolic profiles affected pro-inflammatory factor levels and thus affected tight junction protein levels.
34382058	3	1	theme	related	599:605	arg1	mechanism					607:615	the health effect related mechanism	581:615	the health effect related mechanism	581:615	In the present study, we aim to investigate the effect of oat β-glucan on gut microbiota in colitis mice and explore the health effect related mechanism.
34382058	6	2	theme	Histological	812:823	arg1	evaluation					825:834	Histological evaluation	812:834	Histological evaluation of H&E and TUNEL staining	812:860	Histological evaluation of H&E and TUNEL staining showed that β-glucan treatment suppressed DSS-induced colonic inflammatory infiltration and reduced cell apoptosis levels of colon tissues.
34382058	8	3	theme	β-glucan	1122:1129	arg1	treatment					1131:1139	β-glucan treatment	1122:1139	β-glucan treatment	1122:1139	Moreover, β-glucan treatment increased the protein and mRNA expression levels of tight junction proteins.
34382058	12	4	theme	factor	1805:1810	arg1	levels					1812:1817	pro-inflammatory factor levels	1788:1817	pro-inflammatory factor levels	1788:1817	In contrast, the changes in metabolic profiles affected pro-inflammatory factor levels and thus affected tight junction protein levels.
34382058	8	5	theme	tight	1193:1197	arg1	proteins					1208:1215	tight junction proteins	1193:1215	tight junction proteins	1193:1215	Moreover, β-glucan treatment increased the protein and mRNA expression levels of tight junction proteins.
34382058	10	6	theme	colitis	1558:1564	arg1	mice					1566:1569	colitis mice	1558:1569	colitis mice	1558:1569	Further analysis of gut microbial metabolism revealed that β-glucan treatment significantly increased acetate, propionate and butyrate concentrations, and affected microbial metabolome in colitis mice.
34382058	11	7	theme	expression	1679:1688	arg1	levels					1690:1695	pro-inflammatory factor expression levels	1655:1695	pro-inflammatory factor expression levels	1655:1695	Notably, the increased acetate and propionate concentrations could directly affect pro-inflammatory factor expression levels and tight junction protein levels.
34382058	7	8	theme	factors	1049:1055	arg1	levels					1018:1023	mRNA expression levels	1002:1023	mRNA expression levels of the pro-inflammatory factors	1002:1055	mRNA expression levels of the pro-inflammatory factors were also significantly reduced in the β-glucan group.
34382058	10	9	theme	gut	1390:1392	arg1	metabolism					1404:1413	gut microbial metabolism	1390:1413	gut microbial metabolism	1390:1413	Further analysis of gut microbial metabolism revealed that β-glucan treatment significantly increased acetate, propionate and butyrate concentrations, and affected microbial metabolome in colitis mice.
34382058	3	10	theme	present	471:477	arg1	study					479:483	the present study	467:483	the present study	467:483	In the present study, we aim to investigate the effect of oat β-glucan on gut microbiota in colitis mice and explore the health effect related mechanism.
34382058	1	11	theme	bowel	160:164	arg1	diseases					166:173	the most prevalent inflammatory bowel diseases	128:173	the most prevalent inflammatory bowel diseases (IBD) worldwide	128:189	Ulcerative colitis (UC) is one of the most prevalent inflammatory bowel diseases (IBD) worldwide, while oat β-glucan has been shown to suppress the progress of colitis in UC mice.
34382058	1	11	theme	bowel	160:164	arg1	IBD					176:178	IBD	176:178	IBD	176:178	Ulcerative colitis (UC) is one of the most prevalent inflammatory bowel diseases (IBD) worldwide, while oat β-glucan has been shown to suppress the progress of colitis in UC mice.
34382058	8	12	theme	proteins	1208:1215	arg1	levels					1183:1188	the protein and mRNA expression levels	1151:1188	the protein and mRNA expression levels of tight junction proteins	1151:1215	Moreover, β-glucan treatment increased the protein and mRNA expression levels of tight junction proteins.
34382058	1	13	from	progress	242:249	arg1	mice					268:271	UC mice	265:271	UC mice	265:271	Ulcerative colitis (UC) is one of the most prevalent inflammatory bowel diseases (IBD) worldwide, while oat β-glucan has been shown to suppress the progress of colitis in UC mice.
34382058	13	14	theme	fatty	2012:2016	arg1	SCFAs					2025:2029	SCFAs	2025:2029	SCFAs	2025:2029	Overall, our study revealed that oat β-glucan ameliorated DSS-induced colitis in mice simultaneously through regulating gut-derived short-chain fatty acids (SCFAs) and microbial metabolic biomarkers.
34382058	13	14	theme	fatty	2012:2016	arg1	acids					2018:2022	gut-derived short-chain fatty acids	1988:2022	gut-derived short-chain fatty acids (SCFAs)	1988:2030	Overall, our study revealed that oat β-glucan ameliorated DSS-induced colitis in mice simultaneously through regulating gut-derived short-chain fatty acids (SCFAs) and microbial metabolic biomarkers.
34382058	8	15	theme	protein	1155:1161	arg1	levels					1183:1188	the protein and mRNA expression levels	1151:1188	the protein and mRNA expression levels of tight junction proteins	1151:1215	Moreover, β-glucan treatment increased the protein and mRNA expression levels of tight junction proteins.
34382058	12	16	theme	metabolic	1760:1768	arg1	profiles					1770:1777	metabolic profiles	1760:1777	metabolic profiles	1760:1777	In contrast, the changes in metabolic profiles affected pro-inflammatory factor levels and thus affected tight junction protein levels.
34382058	8	17	theme	mRNA	1167:1170	arg1	levels					1183:1188	the protein and mRNA expression levels	1151:1188	the protein and mRNA expression levels of tight junction proteins	1151:1215	Moreover, β-glucan treatment increased the protein and mRNA expression levels of tight junction proteins.
34382058	7	18	theme	mRNA	1002:1005	arg1	levels					1018:1023	mRNA expression levels	1002:1023	mRNA expression levels of the pro-inflammatory factors	1002:1055	mRNA expression levels of the pro-inflammatory factors were also significantly reduced in the β-glucan group.
34382058	3	19	theme	oat	522:524	arg1	β-glucan					526:533	oat β-glucan	522:533	oat β-glucan	522:533	In the present study, we aim to investigate the effect of oat β-glucan on gut microbiota in colitis mice and explore the health effect related mechanism.
34382058	5	20	from	hematochezia	747:758	arg1	mice					806:809	colitis mice	798:809	colitis mice	798:809	The results showed that β-glucan treatment attenuated hematochezia, splenomegaly and colon shortening in colitis mice.
34382058	6	21	theme	staining	853:860	arg1	evaluation					825:834	Histological evaluation	812:834	Histological evaluation of H&E and TUNEL staining	812:860	Histological evaluation of H&E and TUNEL staining showed that β-glucan treatment suppressed DSS-induced colonic inflammatory infiltration and reduced cell apoptosis levels of colon tissues.
34382058	3	22	from	aim	489:491	arg1	study					479:483	the present study	467:483	the present study	467:483	In the present study, we aim to investigate the effect of oat β-glucan on gut microbiota in colitis mice and explore the health effect related mechanism.
34382058	5	23	from	colon	778:782	arg1	mice					806:809	colitis mice	798:809	colitis mice	798:809	The results showed that β-glucan treatment attenuated hematochezia, splenomegaly and colon shortening in colitis mice.
34382058	6	24	theme	cell	962:965	arg1	levels					977:982	reduced cell apoptosis levels	954:982	reduced cell apoptosis levels of colon tissues	954:999	Histological evaluation of H&E and TUNEL staining showed that β-glucan treatment suppressed DSS-induced colonic inflammatory infiltration and reduced cell apoptosis levels of colon tissues.
34382058	0	25	theme	microbiota	63:72	arg1	metabolism					74:83	gut microbiota metabolism	59:83	gut microbiota metabolism in mice	59:91	Oat β-glucan alleviates DSS-induced colitis via regulating gut microbiota metabolism in mice.
34382058	9	26	theme	microbiota	1234:1243	arg1	community					1245:1253	gut microbiota community	1230:1253	gut microbiota community	1230:1253	Analysis of gut microbiota community showed that β-glucan treatment modulated gut microbial composition and structure at the OTU level in colitis mice.
34382058	5	27	from	splenomegaly	761:772	arg1	mice					806:809	colitis mice	798:809	colitis mice	798:809	The results showed that β-glucan treatment attenuated hematochezia, splenomegaly and colon shortening in colitis mice.
34382058	5	28	theme	colitis	798:804	arg1	mice					806:809	colitis mice	798:809	colitis mice	798:809	The results showed that β-glucan treatment attenuated hematochezia, splenomegaly and colon shortening in colitis mice.
34382058	2	29	theme	β-glucan	422:429	arg1	effect					408:413	the protective effect	393:413	the protective effect of oat β-glucan against colitis	393:445	However, the underlying mechanism of oat β-glucan in ameliorating colitis is unclear and the role of gut microbiota in the protective effect of oat β-glucan against colitis remains unknown.
34382058	4	30	theme	Dextran	618:624	arg1	sodium					634:639	Dextran sulfate sodium	618:639	Dextran sulfate sodium (DSS)	618:645	Dextran sulfate sodium (DSS) was used to induce the colitis model in mice.
34382058	4	30	theme	Dextran	618:624	arg1	DSS					642:644	DSS	642:644	DSS	642:644	Dextran sulfate sodium (DSS) was used to induce the colitis model in mice.
34382058	13	31	theme	DSS-induced	1926:1936	arg1	colitis					1938:1944	DSS-induced colitis	1926:1944	DSS-induced colitis in mice	1926:1952	Overall, our study revealed that oat β-glucan ameliorated DSS-induced colitis in mice simultaneously through regulating gut-derived short-chain fatty acids (SCFAs) and microbial metabolic biomarkers.
34382058	14	32	theme	nutritional	2131:2141	arg1	strategy					2156:2163	an effective nutritional intervention strategy	2118:2163	an effective nutritional intervention strategy towards targeting gut microbiota metabolism for ameliorating colitis	2118:2232	Our study demonstrated that oat β-glucan could be an effective nutritional intervention strategy towards targeting gut microbiota metabolism for ameliorating colitis.
34382058	14	32	theme	nutritional	2131:2141	arg1	β-glucan					2100:2107	oat β-glucan	2096:2107	oat β-glucan	2096:2107	Our study demonstrated that oat β-glucan could be an effective nutritional intervention strategy towards targeting gut microbiota metabolism for ameliorating colitis.
34382058	9	33	from	level	1347:1351	arg1	mice					1364:1367	colitis mice	1356:1367	colitis mice	1356:1367	Analysis of gut microbiota community showed that β-glucan treatment modulated gut microbial composition and structure at the OTU level in colitis mice.
34382058	4	34	from	model	678:682	arg1	mice					687:690	mice	687:690	mice	687:690	Dextran sulfate sodium (DSS) was used to induce the colitis model in mice.
34382058	1	35	theme	prevalent	137:145	arg1	diseases					166:173	the most prevalent inflammatory bowel diseases	128:173	the most prevalent inflammatory bowel diseases (IBD) worldwide	128:189	Ulcerative colitis (UC) is one of the most prevalent inflammatory bowel diseases (IBD) worldwide, while oat β-glucan has been shown to suppress the progress of colitis in UC mice.
34382058	1	35	theme	prevalent	137:145	arg1	IBD					176:178	IBD	176:178	IBD	176:178	Ulcerative colitis (UC) is one of the most prevalent inflammatory bowel diseases (IBD) worldwide, while oat β-glucan has been shown to suppress the progress of colitis in UC mice.
34382058	6	36	theme	inflammatory	924:935	arg1	infiltration					937:948	DSS-induced colonic inflammatory infiltration	904:948	DSS-induced colonic inflammatory infiltration	904:948	Histological evaluation of H&E and TUNEL staining showed that β-glucan treatment suppressed DSS-induced colonic inflammatory infiltration and reduced cell apoptosis levels of colon tissues.
34382058	10	37	theme	metabolism	1404:1413	arg1	analysis					1378:1385	Further analysis	1370:1385	Further analysis of gut microbial metabolism	1370:1413	Further analysis of gut microbial metabolism revealed that β-glucan treatment significantly increased acetate, propionate and butyrate concentrations, and affected microbial metabolome in colitis mice.
34382058	9	38	theme	β-glucan	1267:1274	arg1	treatment					1276:1284	β-glucan treatment	1267:1284	β-glucan treatment	1267:1284	Analysis of gut microbiota community showed that β-glucan treatment modulated gut microbial composition and structure at the OTU level in colitis mice.
34382058	6	39	theme	DSS-induced	904:914	arg1	infiltration					937:948	DSS-induced colonic inflammatory infiltration	904:948	DSS-induced colonic inflammatory infiltration	904:948	Histological evaluation of H&E and TUNEL staining showed that β-glucan treatment suppressed DSS-induced colonic inflammatory infiltration and reduced cell apoptosis levels of colon tissues.
34382058	14	40	theme	gut	2183:2185	arg1	metabolism					2198:2207	gut microbiota metabolism	2183:2207	gut microbiota metabolism	2183:2207	Our study demonstrated that oat β-glucan could be an effective nutritional intervention strategy towards targeting gut microbiota metabolism for ameliorating colitis.
34382058	0	41	theme	Oat	0:2	arg1	β-glucan					4:11	Oat β-glucan	0:11	Oat β-glucan	0:11	Oat β-glucan alleviates DSS-induced colitis via regulating gut microbiota metabolism in mice.
34382058	9	42	theme	microbial	1300:1308	arg1	composition					1310:1320	gut microbial composition	1296:1320	gut microbial composition	1296:1320	Analysis of gut microbiota community showed that β-glucan treatment modulated gut microbial composition and structure at the OTU level in colitis mice.
34382058	1	43	theme	Ulcerative	94:103	arg1	UC					114:115	UC	114:115	UC	114:115	Ulcerative colitis (UC) is one of the most prevalent inflammatory bowel diseases (IBD) worldwide, while oat β-glucan has been shown to suppress the progress of colitis in UC mice.
34382058	1	43	theme	Ulcerative	94:103	arg1	colitis					105:111	Ulcerative colitis	94:111	Ulcerative colitis (UC)	94:116	Ulcerative colitis (UC) is one of the most prevalent inflammatory bowel diseases (IBD) worldwide, while oat β-glucan has been shown to suppress the progress of colitis in UC mice.
34382058	2	44	theme	gut	375:377	arg1	microbiota					379:388	gut microbiota	375:388	gut microbiota	375:388	However, the underlying mechanism of oat β-glucan in ameliorating colitis is unclear and the role of gut microbiota in the protective effect of oat β-glucan against colitis remains unknown.
34382058	14	45	theme	intervention	2143:2154	arg1	strategy					2156:2163	an effective nutritional intervention strategy	2118:2163	an effective nutritional intervention strategy towards targeting gut microbiota metabolism for ameliorating colitis	2118:2232	Our study demonstrated that oat β-glucan could be an effective nutritional intervention strategy towards targeting gut microbiota metabolism for ameliorating colitis.
34382058	14	45	theme	intervention	2143:2154	arg1	β-glucan					2100:2107	oat β-glucan	2096:2107	oat β-glucan	2096:2107	Our study demonstrated that oat β-glucan could be an effective nutritional intervention strategy towards targeting gut microbiota metabolism for ameliorating colitis.
34382058	3	46	from	effect	512:517	arg1	microbiota					542:551	gut microbiota	538:551	gut microbiota	538:551	In the present study, we aim to investigate the effect of oat β-glucan on gut microbiota in colitis mice and explore the health effect related mechanism.
34382058	5	47	theme	β-glucan	717:724	arg1	treatment					726:734	β-glucan treatment	717:734	β-glucan treatment	717:734	The results showed that β-glucan treatment attenuated hematochezia, splenomegaly and colon shortening in colitis mice.
34382058	6	48	theme	colon	987:991	arg1	tissues					993:999	colon tissues	987:999	colon tissues	987:999	Histological evaluation of H&E and TUNEL staining showed that β-glucan treatment suppressed DSS-induced colonic inflammatory infiltration and reduced cell apoptosis levels of colon tissues.
34382058	14	49	theme	effective	2121:2129	arg1	strategy					2156:2163	an effective nutritional intervention strategy	2118:2163	an effective nutritional intervention strategy towards targeting gut microbiota metabolism for ameliorating colitis	2118:2232	Our study demonstrated that oat β-glucan could be an effective nutritional intervention strategy towards targeting gut microbiota metabolism for ameliorating colitis.
34382058	14	49	theme	effective	2121:2129	arg1	β-glucan					2100:2107	oat β-glucan	2096:2107	oat β-glucan	2096:2107	Our study demonstrated that oat β-glucan could be an effective nutritional intervention strategy towards targeting gut microbiota metabolism for ameliorating colitis.
34382058	12	50	theme	protein	1852:1858	arg1	levels					1860:1865	thus affected tight junction protein levels	1823:1865	thus affected tight junction protein levels	1823:1865	In contrast, the changes in metabolic profiles affected pro-inflammatory factor levels and thus affected tight junction protein levels.
34382058	12	51	from	changes	1749:1755	arg1	profiles					1770:1777	metabolic profiles	1760:1777	metabolic profiles	1760:1777	In contrast, the changes in metabolic profiles affected pro-inflammatory factor levels and thus affected tight junction protein levels.
34382058	2	52	theme	protective	397:406	arg1	effect					408:413	the protective effect	393:413	the protective effect of oat β-glucan against colitis	393:445	However, the underlying mechanism of oat β-glucan in ameliorating colitis is unclear and the role of gut microbiota in the protective effect of oat β-glucan against colitis remains unknown.
34382058	3	53	theme	colitis	556:562	arg1	mice					564:567	colitis mice	556:567	colitis mice	556:567	In the present study, we aim to investigate the effect of oat β-glucan on gut microbiota in colitis mice and explore the health effect related mechanism.
34382058	11	54	theme	acetate	1595:1601	arg1	concentrations					1618:1631	the increased acetate and propionate concentrations	1581:1631	the increased acetate and propionate concentrations	1581:1631	Notably, the increased acetate and propionate concentrations could directly affect pro-inflammatory factor expression levels and tight junction protein levels.
34382058	2	55	theme	underlying	287:296	arg1	unclear					351:357	unclear	351:357	unclear	351:357	However, the underlying mechanism of oat β-glucan in ameliorating colitis is unclear and the role of gut microbiota in the protective effect of oat β-glucan against colitis remains unknown.
34382058	2	55	theme	underlying	287:296	arg1	mechanism					298:306	the underlying mechanism	283:306	the underlying mechanism of oat β-glucan in ameliorating colitis	283:346	However, the underlying mechanism of oat β-glucan in ameliorating colitis is unclear and the role of gut microbiota in the protective effect of oat β-glucan against colitis remains unknown.
34382058	9	56	theme	OTU	1343:1345	arg1	level					1347:1351	the OTU level	1339:1351	the OTU level in colitis mice	1339:1367	Analysis of gut microbiota community showed that β-glucan treatment modulated gut microbial composition and structure at the OTU level in colitis mice.
34382058	13	57	theme	gut-derived	1988:1998	arg1	SCFAs					2025:2029	SCFAs	2025:2029	SCFAs	2025:2029	Overall, our study revealed that oat β-glucan ameliorated DSS-induced colitis in mice simultaneously through regulating gut-derived short-chain fatty acids (SCFAs) and microbial metabolic biomarkers.
34382058	13	57	theme	gut-derived	1988:1998	arg1	acids					2018:2022	gut-derived short-chain fatty acids	1988:2022	gut-derived short-chain fatty acids (SCFAs)	1988:2030	Overall, our study revealed that oat β-glucan ameliorated DSS-induced colitis in mice simultaneously through regulating gut-derived short-chain fatty acids (SCFAs) and microbial metabolic biomarkers.
34382058	5	58	dep	hematochezia	747:758	arg1	shortening					784:793	shortening	784:793	shortening	784:793	The results showed that β-glucan treatment attenuated hematochezia, splenomegaly and colon shortening in colitis mice.
34382058	13	59	theme	metabolic	2046:2054	arg1	biomarkers					2056:2065	microbial metabolic biomarkers	2036:2065	microbial metabolic biomarkers	2036:2065	Overall, our study revealed that oat β-glucan ameliorated DSS-induced colitis in mice simultaneously through regulating gut-derived short-chain fatty acids (SCFAs) and microbial metabolic biomarkers.
34382058	2	60	theme	β-glucan	315:322	arg1	unclear					351:357	unclear	351:357	unclear	351:357	However, the underlying mechanism of oat β-glucan in ameliorating colitis is unclear and the role of gut microbiota in the protective effect of oat β-glucan against colitis remains unknown.
34382058	2	60	theme	β-glucan	315:322	arg1	mechanism					298:306	the underlying mechanism	283:306	the underlying mechanism of oat β-glucan in ameliorating colitis	283:346	However, the underlying mechanism of oat β-glucan in ameliorating colitis is unclear and the role of gut microbiota in the protective effect of oat β-glucan against colitis remains unknown.
34382058	13	61	link	gut-derived	1988:1998	arg1	SCFAs					2025:2029	SCFAs	2025:2029	SCFAs	2025:2029	Overall, our study revealed that oat β-glucan ameliorated DSS-induced colitis in mice simultaneously through regulating gut-derived short-chain fatty acids (SCFAs) and microbial metabolic biomarkers.
34382058	13	61	link	gut-derived	1988:1998	arg1	acids					2018:2022	gut-derived short-chain fatty acids	1988:2022	gut-derived short-chain fatty acids (SCFAs)	1988:2030	Overall, our study revealed that oat β-glucan ameliorated DSS-induced colitis in mice simultaneously through regulating gut-derived short-chain fatty acids (SCFAs) and microbial metabolic biomarkers.
34382058	6	62	theme	H&E	839:841	arg1	staining					853:860	H&E and TUNEL staining	839:860	staining	853:860	Histological evaluation of H&E and TUNEL staining showed that β-glucan treatment suppressed DSS-induced colonic inflammatory infiltration and reduced cell apoptosis levels of colon tissues.
34382058	2	63	from	role	367:370	arg1	effect					408:413	the protective effect	393:413	the protective effect of oat β-glucan against colitis	393:445	However, the underlying mechanism of oat β-glucan in ameliorating colitis is unclear and the role of gut microbiota in the protective effect of oat β-glucan against colitis remains unknown.
34382058	10	64	theme	microbial	1534:1542	arg1	metabolome					1544:1553	microbial metabolome	1534:1553	microbial metabolome in colitis mice	1534:1569	Further analysis of gut microbial metabolism revealed that β-glucan treatment significantly increased acetate, propionate and butyrate concentrations, and affected microbial metabolome in colitis mice.
34382058	11	65	theme	factor	1672:1677	arg1	levels					1690:1695	pro-inflammatory factor expression levels	1655:1695	pro-inflammatory factor expression levels	1655:1695	Notably, the increased acetate and propionate concentrations could directly affect pro-inflammatory factor expression levels and tight junction protein levels.
34382058	11	66	theme	propionate	1607:1616	arg1	concentrations					1618:1631	the increased acetate and propionate concentrations	1581:1631	the increased acetate and propionate concentrations	1581:1631	Notably, the increased acetate and propionate concentrations could directly affect pro-inflammatory factor expression levels and tight junction protein levels.
34382058	12	67	theme	pro-inflammatory	1788:1803	arg1	levels					1812:1817	pro-inflammatory factor levels	1788:1817	pro-inflammatory factor levels	1788:1817	In contrast, the changes in metabolic profiles affected pro-inflammatory factor levels and thus affected tight junction protein levels.
34382058	11	68	theme	protein	1716:1722	arg1	levels					1724:1729	tight junction protein levels	1701:1729	tight junction protein levels	1701:1729	Notably, the increased acetate and propionate concentrations could directly affect pro-inflammatory factor expression levels and tight junction protein levels.
34382058	11	69	theme	tight	1701:1705	arg1	levels					1724:1729	tight junction protein levels	1701:1729	tight junction protein levels	1701:1729	Notably, the increased acetate and propionate concentrations could directly affect pro-inflammatory factor expression levels and tight junction protein levels.
34382058	7	70	theme	pro-inflammatory	1032:1047	arg1	factors					1049:1055	the pro-inflammatory factors	1028:1055	the pro-inflammatory factors	1028:1055	mRNA expression levels of the pro-inflammatory factors were also significantly reduced in the β-glucan group.
34382058	1	71	theme	inflammatory	147:158	arg1	diseases					166:173	the most prevalent inflammatory bowel diseases	128:173	the most prevalent inflammatory bowel diseases (IBD) worldwide	128:189	Ulcerative colitis (UC) is one of the most prevalent inflammatory bowel diseases (IBD) worldwide, while oat β-glucan has been shown to suppress the progress of colitis in UC mice.
34382058	1	71	theme	inflammatory	147:158	arg1	IBD					176:178	IBD	176:178	IBD	176:178	Ulcerative colitis (UC) is one of the most prevalent inflammatory bowel diseases (IBD) worldwide, while oat β-glucan has been shown to suppress the progress of colitis in UC mice.
34382058	13	72	theme	short-chain	2000:2010	arg1	SCFAs					2025:2029	SCFAs	2025:2029	SCFAs	2025:2029	Overall, our study revealed that oat β-glucan ameliorated DSS-induced colitis in mice simultaneously through regulating gut-derived short-chain fatty acids (SCFAs) and microbial metabolic biomarkers.
34382058	13	72	theme	short-chain	2000:2010	arg1	acids					2018:2022	gut-derived short-chain fatty acids	1988:2022	gut-derived short-chain fatty acids (SCFAs)	1988:2030	Overall, our study revealed that oat β-glucan ameliorated DSS-induced colitis in mice simultaneously through regulating gut-derived short-chain fatty acids (SCFAs) and microbial metabolic biomarkers.
34382058	1	73	theme	diseases	166:173	arg1	worldwide					181:189	the most prevalent inflammatory bowel diseases (IBD) worldwide	128:189	the most prevalent inflammatory bowel diseases (IBD) worldwide	128:189	Ulcerative colitis (UC) is one of the most prevalent inflammatory bowel diseases (IBD) worldwide, while oat β-glucan has been shown to suppress the progress of colitis in UC mice.
34382058	8	74	theme	junction	1199:1206	arg1	proteins					1208:1215	tight junction proteins	1193:1215	tight junction proteins	1193:1215	Moreover, β-glucan treatment increased the protein and mRNA expression levels of tight junction proteins.
34382058	7	75	theme	expression	1007:1016	arg1	levels					1018:1023	mRNA expression levels	1002:1023	mRNA expression levels of the pro-inflammatory factors	1002:1055	mRNA expression levels of the pro-inflammatory factors were also significantly reduced in the β-glucan group.
34382058	10	76	theme	Further	1370:1376	arg1	analysis					1378:1385	Further analysis	1370:1385	Further analysis of gut microbial metabolism	1370:1413	Further analysis of gut microbial metabolism revealed that β-glucan treatment significantly increased acetate, propionate and butyrate concentrations, and affected microbial metabolome in colitis mice.
34382058	6	77	theme	β-glucan	874:881	arg1	treatment					883:891	β-glucan treatment	874:891	β-glucan treatment	874:891	Histological evaluation of H&E and TUNEL staining showed that β-glucan treatment suppressed DSS-induced colonic inflammatory infiltration and reduced cell apoptosis levels of colon tissues.
34382058	1	78	theme	worldwide	181:189	arg1	one					121:123	one	121:123	one	121:123	Ulcerative colitis (UC) is one of the most prevalent inflammatory bowel diseases (IBD) worldwide, while oat β-glucan has been shown to suppress the progress of colitis in UC mice.
34382058	1	78	theme	worldwide	181:189	arg1	worldwide					181:189	the most prevalent inflammatory bowel diseases (IBD) worldwide	128:189	the most prevalent inflammatory bowel diseases (IBD) worldwide	128:189	Ulcerative colitis (UC) is one of the most prevalent inflammatory bowel diseases (IBD) worldwide, while oat β-glucan has been shown to suppress the progress of colitis in UC mice.
34382058	0	79	from	metabolism	74:83	arg1	mice					88:91	mice	88:91	mice	88:91	Oat β-glucan alleviates DSS-induced colitis via regulating gut microbiota metabolism in mice.
34382058	8	80	theme	expression	1172:1181	arg1	levels					1183:1188	the protein and mRNA expression levels	1151:1188	the protein and mRNA expression levels of tight junction proteins	1151:1215	Moreover, β-glucan treatment increased the protein and mRNA expression levels of tight junction proteins.
34382058	6	81	theme	TUNEL	847:851	arg1	staining					853:860	H&E and TUNEL staining	839:860	staining	853:860	Histological evaluation of H&E and TUNEL staining showed that β-glucan treatment suppressed DSS-induced colonic inflammatory infiltration and reduced cell apoptosis levels of colon tissues.
34382058	4	82	theme	sulfate	626:632	arg1	sodium					634:639	Dextran sulfate sodium	618:639	Dextran sulfate sodium (DSS)	618:645	Dextran sulfate sodium (DSS) was used to induce the colitis model in mice.
34382058	4	82	theme	sulfate	626:632	arg1	DSS					642:644	DSS	642:644	DSS	642:644	Dextran sulfate sodium (DSS) was used to induce the colitis model in mice.
34382058	3	83	theme	gut	538:540	arg1	microbiota					542:551	gut microbiota	538:551	gut microbiota	538:551	In the present study, we aim to investigate the effect of oat β-glucan on gut microbiota in colitis mice and explore the health effect related mechanism.
34382058	6	84	theme	apoptosis	967:975	arg1	levels					977:982	reduced cell apoptosis levels	954:982	reduced cell apoptosis levels of colon tissues	954:999	Histological evaluation of H&E and TUNEL staining showed that β-glucan treatment suppressed DSS-induced colonic inflammatory infiltration and reduced cell apoptosis levels of colon tissues.
34382058	9	85	theme	gut	1230:1232	arg1	community					1245:1253	gut microbiota community	1230:1253	gut microbiota community	1230:1253	Analysis of gut microbiota community showed that β-glucan treatment modulated gut microbial composition and structure at the OTU level in colitis mice.
34382058	3	86	theme	β-glucan	526:533	arg1	effect					512:517	the effect	508:517	the effect of oat β-glucan on gut microbiota	508:551	In the present study, we aim to investigate the effect of oat β-glucan on gut microbiota in colitis mice and explore the health effect related mechanism.
34382058	6	87	theme	reduced	954:960	arg1	levels					977:982	reduced cell apoptosis levels	954:982	reduced cell apoptosis levels of colon tissues	954:999	Histological evaluation of H&E and TUNEL staining showed that β-glucan treatment suppressed DSS-induced colonic inflammatory infiltration and reduced cell apoptosis levels of colon tissues.
34382058	9	88	theme	community	1245:1253	arg1	Analysis					1218:1225	Analysis	1218:1225	Analysis of gut microbiota community	1218:1253	Analysis of gut microbiota community showed that β-glucan treatment modulated gut microbial composition and structure at the OTU level in colitis mice.
34382058	14	89	theme	oat	2096:2098	arg1	strategy					2156:2163	an effective nutritional intervention strategy	2118:2163	an effective nutritional intervention strategy towards targeting gut microbiota metabolism for ameliorating colitis	2118:2232	Our study demonstrated that oat β-glucan could be an effective nutritional intervention strategy towards targeting gut microbiota metabolism for ameliorating colitis.
34382058	14	89	theme	oat	2096:2098	arg1	β-glucan					2100:2107	oat β-glucan	2096:2107	oat β-glucan	2096:2107	Our study demonstrated that oat β-glucan could be an effective nutritional intervention strategy towards targeting gut microbiota metabolism for ameliorating colitis.
34382058	2	90	theme	oat	418:420	arg1	β-glucan					422:429	oat β-glucan	418:429	oat β-glucan	418:429	However, the underlying mechanism of oat β-glucan in ameliorating colitis is unclear and the role of gut microbiota in the protective effect of oat β-glucan against colitis remains unknown.
34382058	14	91	theme	microbiota	2187:2196	arg1	metabolism					2198:2207	gut microbiota metabolism	2183:2207	gut microbiota metabolism	2183:2207	Our study demonstrated that oat β-glucan could be an effective nutritional intervention strategy towards targeting gut microbiota metabolism for ameliorating colitis.
34382058	13	92	from	colitis	1938:1944	arg1	mice					1949:1952	mice	1949:1952	mice	1949:1952	Overall, our study revealed that oat β-glucan ameliorated DSS-induced colitis in mice simultaneously through regulating gut-derived short-chain fatty acids (SCFAs) and microbial metabolic biomarkers.
34382058	6	93	theme	colonic	916:922	arg1	infiltration					937:948	DSS-induced colonic inflammatory infiltration	904:948	DSS-induced colonic inflammatory infiltration	904:948	Histological evaluation of H&E and TUNEL staining showed that β-glucan treatment suppressed DSS-induced colonic inflammatory infiltration and reduced cell apoptosis levels of colon tissues.
34382058	10	94	theme	microbial	1394:1402	arg1	metabolism					1404:1413	gut microbial metabolism	1390:1413	gut microbial metabolism	1390:1413	Further analysis of gut microbial metabolism revealed that β-glucan treatment significantly increased acetate, propionate and butyrate concentrations, and affected microbial metabolome in colitis mice.
34382058	4	95	theme	colitis	670:676	arg1	model					678:682	the colitis model	666:682	the colitis model in mice	666:690	Dextran sulfate sodium (DSS) was used to induce the colitis model in mice.
34382058	13	96	theme	oat	1901:1903	arg1	β-glucan					1905:1912	oat β-glucan	1901:1912	oat β-glucan	1901:1912	Overall, our study revealed that oat β-glucan ameliorated DSS-induced colitis in mice simultaneously through regulating gut-derived short-chain fatty acids (SCFAs) and microbial metabolic biomarkers.
34382058	9	97	theme	gut	1296:1298	arg1	composition					1310:1320	gut microbial composition	1296:1320	gut microbial composition	1296:1320	Analysis of gut microbiota community showed that β-glucan treatment modulated gut microbial composition and structure at the OTU level in colitis mice.
34382058	2	98	theme	microbiota	379:388	arg1	role					367:370	the role	363:370	the role of gut microbiota in the protective effect of oat β-glucan against colitis	363:445	However, the underlying mechanism of oat β-glucan in ameliorating colitis is unclear and the role of gut microbiota in the protective effect of oat β-glucan against colitis remains unknown.
34382058	10	99	theme	β-glucan	1429:1436	arg1	treatment					1438:1446	β-glucan treatment	1429:1446	β-glucan treatment	1429:1446	Further analysis of gut microbial metabolism revealed that β-glucan treatment significantly increased acetate, propionate and butyrate concentrations, and affected microbial metabolome in colitis mice.
34382058	0	100	theme	DSS-induced	24:34	arg1	colitis					36:42	DSS-induced colitis	24:42	DSS-induced colitis	24:42	Oat β-glucan alleviates DSS-induced colitis via regulating gut microbiota metabolism in mice.
34382058	6	101	theme	tissues	993:999	arg1	levels					977:982	reduced cell apoptosis levels	954:982	reduced cell apoptosis levels of colon tissues	954:999	Histological evaluation of H&E and TUNEL staining showed that β-glucan treatment suppressed DSS-induced colonic inflammatory infiltration and reduced cell apoptosis levels of colon tissues.
34382058	6	101	theme	tissues	993:999	arg1	infiltration					937:948	DSS-induced colonic inflammatory infiltration	904:948	DSS-induced colonic inflammatory infiltration	904:948	Histological evaluation of H&E and TUNEL staining showed that β-glucan treatment suppressed DSS-induced colonic inflammatory infiltration and reduced cell apoptosis levels of colon tissues.
34382058	10	102	theme	propionate	1481:1490	arg1	concentrations					1505:1518	acetate, propionate and butyrate concentrations	1472:1518	acetate, propionate and butyrate concentrations	1472:1518	Further analysis of gut microbial metabolism revealed that β-glucan treatment significantly increased acetate, propionate and butyrate concentrations, and affected microbial metabolome in colitis mice.
34382058	7	103	theme	β-glucan	1096:1103	arg1	group					1105:1109	the β-glucan group	1092:1109	the β-glucan group	1092:1109	mRNA expression levels of the pro-inflammatory factors were also significantly reduced in the β-glucan group.
34382058	1	104	theme	colitis	254:260	arg1	progress					242:249	the progress	238:249	the progress of colitis in UC mice	238:271	Ulcerative colitis (UC) is one of the most prevalent inflammatory bowel diseases (IBD) worldwide, while oat β-glucan has been shown to suppress the progress of colitis in UC mice.
34382058	10	105	theme	acetate	1472:1478	arg1	concentrations					1505:1518	acetate, propionate and butyrate concentrations	1472:1518	acetate, propionate and butyrate concentrations	1472:1518	Further analysis of gut microbial metabolism revealed that β-glucan treatment significantly increased acetate, propionate and butyrate concentrations, and affected microbial metabolome in colitis mice.
34382058	11	106	theme	junction	1707:1714	arg1	levels					1724:1729	tight junction protein levels	1701:1729	tight junction protein levels	1701:1729	Notably, the increased acetate and propionate concentrations could directly affect pro-inflammatory factor expression levels and tight junction protein levels.
34382058	11	107	theme	increased	1585:1593	arg1	concentrations					1618:1631	the increased acetate and propionate concentrations	1581:1631	the increased acetate and propionate concentrations	1581:1631	Notably, the increased acetate and propionate concentrations could directly affect pro-inflammatory factor expression levels and tight junction protein levels.
34382058	1	108	theme	oat	198:200	arg1	β-glucan					202:209	oat β-glucan	198:209	oat β-glucan	198:209	Ulcerative colitis (UC) is one of the most prevalent inflammatory bowel diseases (IBD) worldwide, while oat β-glucan has been shown to suppress the progress of colitis in UC mice.
34382058	0	109	theme	gut	59:61	arg1	metabolism					74:83	gut microbiota metabolism	59:83	gut microbiota metabolism in mice	59:91	Oat β-glucan alleviates DSS-induced colitis via regulating gut microbiota metabolism in mice.
34382058	1	110	theme	UC	265:266	arg1	mice					268:271	UC mice	265:271	UC mice	265:271	Ulcerative colitis (UC) is one of the most prevalent inflammatory bowel diseases (IBD) worldwide, while oat β-glucan has been shown to suppress the progress of colitis in UC mice.
34382058	10	111	from	metabolome	1544:1553	arg1	mice					1566:1569	colitis mice	1558:1569	colitis mice	1558:1569	Further analysis of gut microbial metabolism revealed that β-glucan treatment significantly increased acetate, propionate and butyrate concentrations, and affected microbial metabolome in colitis mice.
34382058	12	112	theme	junction	1843:1850	arg1	levels					1860:1865	thus affected tight junction protein levels	1823:1865	thus affected tight junction protein levels	1823:1865	In contrast, the changes in metabolic profiles affected pro-inflammatory factor levels and thus affected tight junction protein levels.
34382058	3	113	theme	health	585:590	arg1	effect					592:597	health effect	585:597	health effect	585:597	In the present study, we aim to investigate the effect of oat β-glucan on gut microbiota in colitis mice and explore the health effect related mechanism.
34382058	12	114	theme	tight	1837:1841	arg1	levels					1860:1865	thus affected tight junction protein levels	1823:1865	thus affected tight junction protein levels	1823:1865	In contrast, the changes in metabolic profiles affected pro-inflammatory factor levels and thus affected tight junction protein levels.
34382058	10	115	theme	butyrate	1496:1503	arg1	concentrations					1505:1518	acetate, propionate and butyrate concentrations	1472:1518	acetate, propionate and butyrate concentrations	1472:1518	Further analysis of gut microbial metabolism revealed that β-glucan treatment significantly increased acetate, propionate and butyrate concentrations, and affected microbial metabolome in colitis mice.
34382058	13	116	theme	microbial	2036:2044	arg1	biomarkers					2056:2065	microbial metabolic biomarkers	2036:2065	microbial metabolic biomarkers	2036:2065	Overall, our study revealed that oat β-glucan ameliorated DSS-induced colitis in mice simultaneously through regulating gut-derived short-chain fatty acids (SCFAs) and microbial metabolic biomarkers.
34382058	9	117	theme	colitis	1356:1362	arg1	mice					1364:1367	colitis mice	1356:1367	colitis mice	1356:1367	Analysis of gut microbiota community showed that β-glucan treatment modulated gut microbial composition and structure at the OTU level in colitis mice.
34382058	11	118	theme	pro-inflammatory	1655:1670	arg1	levels					1690:1695	pro-inflammatory factor expression levels	1655:1695	pro-inflammatory factor expression levels	1655:1695	Notably, the increased acetate and propionate concentrations could directly affect pro-inflammatory factor expression levels and tight junction protein levels.
34382058	2	119	theme	oat	311:313	arg1	β-glucan					315:322	oat β-glucan	311:322	oat β-glucan	311:322	However, the underlying mechanism of oat β-glucan in ameliorating colitis is unclear and the role of gut microbiota in the protective effect of oat β-glucan against colitis remains unknown.
34382058	4	120	used	used	651:654	arg2	DSS					642:644	DSS	642:644	DSS	642:644	Dextran sulfate sodium (DSS) was used to induce the colitis model in mice.
34382058	4	120	used	used	651:654	arg2	sodium					634:639	Dextran sulfate sodium	618:639	Dextran sulfate sodium (DSS)	618:645	Dextran sulfate sodium (DSS) was used to induce the colitis model in mice.
33724115	3	0	theme	fermentation	290:301	arg1	products					303:310	protein fermentation products	282:310	protein fermentation products	282:310	The production of protein fermentation products and metabolites depends on the composition, diversity, and metabolism of the gut microbiota.
33724115	6	1	theme	diseases	819:826	arg1	risk					699:702	the risk	695:702	the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD)	695:832	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	10	2	dep	compounds	1179:1187	arg1	extracts					1196:1203	plant extracts	1190:1203	plant extracts	1190:1203	Natural compounds (plant extracts, whose main constituents are polysaccharides and polyphenols), prebiotics, probiotics, and regular physical exercise improve gut dysbiosis and reduce disease risk.
33724115	3	3	theme	products	303:310	arg1	production					268:277	The production	264:277	The production of protein fermentation products and metabolites	264:326	The production of protein fermentation products and metabolites depends on the composition, diversity, and metabolism of the gut microbiota.
33724115	6	4	theme	cardiovascular	804:817	arg1	CVD					829:831	CVD	829:831	CVD	829:831	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	6	4	theme	cardiovascular	804:817	arg1	diseases					819:826	cardiovascular diseases	804:826	cardiovascular diseases (CVD)	804:832	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	7	5	theme	old	1006:1008	arg1	65 years					997:1004	65 years	997:1004	65 years old	997:1008	Short-term HPDs have little effect on the metabolism of healthy individuals under 65 years old.
33724115	6	6	theme	central	761:767	arg1	CNS					785:787	CNS	785:787	CNS	785:787	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	6	6	theme	central	761:767	arg1	system					777:782	central nervous system	761:782	central nervous system (CNS) diseases	761:797	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	8	7	theme	protein	1032:1038	arg1	requirements					1040:1051	the protein requirements	1028:1051	the protein requirements of individuals over 65 years old using HPDs	1028:1095	However, meeting the protein requirements of individuals over 65 years old using HPDs is more challenging.
33724115	4	8	theme	hydrogen	484:491	arg1	H2S					502:504	H2S	502:504	H2S	502:504	Several protein fermentation products, including indoles, phenols, polyamines, hydrogen sulfide (H2S), amines, and carnitine, are toxic.
33724115	4	8	theme	hydrogen	484:491	arg1	sulfide					493:499	hydrogen sulfide	484:499	hydrogen sulfide (H2S)	484:505	Several protein fermentation products, including indoles, phenols, polyamines, hydrogen sulfide (H2S), amines, and carnitine, are toxic.
33724115	7	9	theme	individuals	979:989	arg1	metabolism					957:966	the metabolism	953:966	the metabolism of healthy individuals under 65 years old	953:1008	Short-term HPDs have little effect on the metabolism of healthy individuals under 65 years old.
33724115	9	10	theme	HPDs	1141:1144	arg1	minimal					1162:1168	minimal	1162:1168	minimal	1162:1168	The adverse effects of HPDs on athletes are minimal.
33724115	9	10	theme	HPDs	1141:1144	arg1	effects					1130:1136	The adverse effects	1118:1136	The adverse effects of HPDs on athletes	1118:1156	The adverse effects of HPDs on athletes are minimal.
33724115	9	11	from	effects	1130:1136	arg1	athletes					1149:1156	athletes	1149:1156	athletes	1149:1156	The adverse effects of HPDs on athletes are minimal.
33724115	10	12	theme	regular	1296:1302	arg1	exercise					1313:1320	regular physical exercise	1296:1320	regular physical exercise	1296:1320	Natural compounds (plant extracts, whose main constituents are polysaccharides and polyphenols), prebiotics, probiotics, and regular physical exercise improve gut dysbiosis and reduce disease risk.
33724115	6	13	theme	diabetes	735:742	arg1	risk					699:702	the risk	695:702	the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD)	695:832	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	0	14	from	microbiota	33:42	arg1	health					53:58	human health	47:58	human health	47:58	High animal protein diet and gut microbiota in human health.
33724115	1	15	theme	research	129:136	arg1	hotspot					138:144	a research hotspot	127:144	a research hotspot	127:144	The role of the intestinal flora in health and disease has become a research hotspot.
33724115	7	16	theme	healthy	971:977	arg1	individuals					979:989	healthy individuals	971:989	healthy individuals	971:989	Short-term HPDs have little effect on the metabolism of healthy individuals under 65 years old.
33724115	6	17	theme	type	728:731	arg1	diabetes					735:742	type 2 diabetes	728:742	type 2 diabetes (T2DM)	728:749	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	6	17	theme	type	728:731	arg1	T2DM					745:748	T2DM	745:748	T2DM	745:748	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	8	18	theme	individuals	1056:1066	arg1	requirements					1040:1051	the protein requirements	1028:1051	the protein requirements of individuals over 65 years old using HPDs	1028:1095	However, meeting the protein requirements of individuals over 65 years old using HPDs is more challenging.
33724115	0	19	theme	animal	5:10	arg1	diet					20:23	High animal protein diet	0:23	High animal protein diet	0:23	High animal protein diet and gut microbiota in human health.
33724115	5	20	theme	high-protein	587:598	arg1	HPDs					607:610	HPDs	607:610	HPDs	607:610	This study analyzes the relationship between high-protein diets (HPDs), the intestinal microbiota, and human health and disease.
33724115	5	20	theme	high-protein	587:598	arg1	diets					600:604	high-protein diets	587:604	high-protein diets (HPDs)	587:611	This study analyzes the relationship between high-protein diets (HPDs), the intestinal microbiota, and human health and disease.
33724115	5	21	theme	intestinal	618:627	arg1	microbiota					629:638	the intestinal microbiota	614:638	the intestinal microbiota	614:638	This study analyzes the relationship between high-protein diets (HPDs), the intestinal microbiota, and human health and disease.
33724115	3	22	theme	microbiota	393:402	arg1	diversity					356:364	diversity	356:364	diversity	356:364	The production of protein fermentation products and metabolites depends on the composition, diversity, and metabolism of the gut microbiota.
33724115	3	22	theme	microbiota	393:402	arg1	metabolism					371:380	metabolism	371:380	metabolism	371:380	The production of protein fermentation products and metabolites depends on the composition, diversity, and metabolism of the gut microbiota.
33724115	3	22	theme	microbiota	393:402	arg1	composition					343:353	composition	343:353	composition	343:353	The production of protein fermentation products and metabolites depends on the composition, diversity, and metabolism of the gut microbiota.
33724115	0	23	theme	High	0:3	arg1	diet					20:23	High animal protein diet	0:23	High animal protein diet	0:23	High animal protein diet and gut microbiota in human health.
33724115	10	24	theme	gut	1330:1332	arg1	dysbiosis					1334:1342	gut dysbiosis	1330:1342	gut dysbiosis	1330:1342	Natural compounds (plant extracts, whose main constituents are polysaccharides and polyphenols), prebiotics, probiotics, and regular physical exercise improve gut dysbiosis and reduce disease risk.
33724115	6	25	theme	diseases	718:725	arg1	risk					699:702	the risk	695:702	the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD)	695:832	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	10	26	theme	plant	1190:1194	arg1	extracts					1196:1203	plant extracts	1190:1203	plant extracts	1190:1203	Natural compounds (plant extracts, whose main constituents are polysaccharides and polyphenols), prebiotics, probiotics, and regular physical exercise improve gut dysbiosis and reduce disease risk.
33724115	6	27	theme	system	777:782	arg1	diseases					790:797	central nervous system (CNS) diseases	761:797	central nervous system (CNS) diseases	761:797	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	8	28	theme	old	1082:1084	arg1	HPDs					1092:1095	65 years old using HPDs	1073:1095	65 years old using HPDs	1073:1095	However, meeting the protein requirements of individuals over 65 years old using HPDs is more challenging.
33724115	6	29	from	metabolites	853:863	arg1	colon					872:876	the colon	868:876	the colon	868:876	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	6	30	theme	intestinal	707:716	arg1	diseases					718:725	intestinal diseases	707:725	intestinal diseases	707:725	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	7	31	contain	have	931:934	arg2	effect					943:948	little effect	936:948	little effect	936:948	Short-term HPDs have little effect on the metabolism of healthy individuals under 65 years old.
33724115	7	31	contain	have	931:934	arg1	HPDs					926:929	Short-term HPDs	915:929	Short-term HPDs	915:929	Short-term HPDs have little effect on the metabolism of healthy individuals under 65 years old.
33724115	0	32	theme	protein	12:18	arg1	diet					20:23	High animal protein diet	0:23	High animal protein diet	0:23	High animal protein diet and gut microbiota in human health.
33724115	5	33	theme	human	645:649	arg1	health					651:656	health	651:656	health	651:656	This study analyzes the relationship between high-protein diets (HPDs), the intestinal microbiota, and human health and disease.
33724115	9	34	theme	adverse	1122:1128	arg1	minimal					1162:1168	minimal	1162:1168	minimal	1162:1168	The adverse effects of HPDs on athletes are minimal.
33724115	9	34	theme	adverse	1122:1128	arg1	effects					1130:1136	The adverse effects	1118:1136	The adverse effects of HPDs on athletes	1118:1156	The adverse effects of HPDs on athletes are minimal.
33724115	1	35	theme	intestinal	77:86	arg1	flora					88:92	the intestinal flora	73:92	the intestinal flora	73:92	The role of the intestinal flora in health and disease has become a research hotspot.
33724115	2	36	from	fermentation	233:244	arg1	intestine					253:261	the intestine	249:261	the intestine	249:261	Compared with carbohydrates and fats, proteins are metabolized primarily by microbial fermentation in the intestine.
33724115	0	37	theme	gut	29:31	arg1	microbiota					33:42	gut microbiota	29:42	gut microbiota in human health	29:58	High animal protein diet and gut microbiota in human health.
33724115	6	38	theme	toxic	847:851	arg1	metabolites					853:863	toxic metabolites	847:863	toxic metabolites	847:863	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	6	38	theme	toxic	847:851	arg1	ammonia					906:912	ammonia	906:912	ammonia	906:912	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	6	38	theme	toxic	847:851	arg1	amines					889:894	amines	889:894	amines	889:894	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	6	38	theme	toxic	847:851	arg1	H2S					897:899	H2S	897:899	H2S	897:899	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	4	39	theme	fermentation	421:432	arg1	products					434:441	Several protein fermentation products	405:441	Several protein fermentation products	405:441	Several protein fermentation products, including indoles, phenols, polyamines, hydrogen sulfide (H2S), amines, and carnitine, are toxic.
33724115	10	40	theme	Natural	1171:1177	arg1	compounds					1179:1187	Natural compounds	1171:1187	Natural compounds (plant extracts, whose main constituents are polysaccharides and polyphenols)	1171:1265	Natural compounds (plant extracts, whose main constituents are polysaccharides and polyphenols), prebiotics, probiotics, and regular physical exercise improve gut dysbiosis and reduce disease risk.
33724115	3	41	theme	gut	389:391	arg1	microbiota					393:402	the gut microbiota	385:402	the gut microbiota	385:402	The production of protein fermentation products and metabolites depends on the composition, diversity, and metabolism of the gut microbiota.
33724115	7	42	theme	little	936:941	arg1	effect					943:948	little effect	936:948	little effect	936:948	Short-term HPDs have little effect on the metabolism of healthy individuals under 65 years old.
33724115	6	43	theme	nervous	769:775	arg1	CNS					785:787	CNS	785:787	CNS	785:787	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	6	43	theme	nervous	769:775	arg1	system					777:782	central nervous system	761:782	central nervous system (CNS) diseases	761:797	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	10	44	theme	main	1212:1215	arg1	polysaccharides					1234:1248	polysaccharides	1234:1248	polysaccharides	1234:1248	Natural compounds (plant extracts, whose main constituents are polysaccharides and polyphenols), prebiotics, probiotics, and regular physical exercise improve gut dysbiosis and reduce disease risk.
33724115	10	44	theme	main	1212:1215	arg1	constituents					1217:1228	main constituents	1212:1228	main constituents	1212:1228	Natural compounds (plant extracts, whose main constituents are polysaccharides and polyphenols), prebiotics, probiotics, and regular physical exercise improve gut dysbiosis and reduce disease risk.
33724115	7	45	theme	Short-term	915:924	arg1	HPDs					926:929	Short-term HPDs	915:929	Short-term HPDs	915:929	Short-term HPDs have little effect on the metabolism of healthy individuals under 65 years old.
33724115	0	46	theme	human	47:51	arg1	health					53:58	human health	47:58	human health	47:58	High animal protein diet and gut microbiota in human health.
33724115	1	47	theme	flora	88:92	arg1	role					65:68	The role	61:68	The role of the intestinal flora in health and disease	61:114	The role of the intestinal flora in health and disease has become a research hotspot.
33724115	10	48	theme	disease	1355:1361	arg1	risk					1363:1366	disease risk	1355:1366	disease risk	1355:1366	Natural compounds (plant extracts, whose main constituents are polysaccharides and polyphenols), prebiotics, probiotics, and regular physical exercise improve gut dysbiosis and reduce disease risk.
33724115	3	49	theme	protein	282:288	arg1	products					303:310	protein fermentation products	282:310	protein fermentation products	282:310	The production of protein fermentation products and metabolites depends on the composition, diversity, and metabolism of the gut microbiota.
33724115	6	50	theme	diseases	790:797	arg1	risk					699:702	the risk	695:702	the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD)	695:832	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	10	51	theme	physical	1304:1311	arg1	exercise					1313:1320	regular physical exercise	1296:1320	regular physical exercise	1296:1320	Natural compounds (plant extracts, whose main constituents are polysaccharides and polyphenols), prebiotics, probiotics, and regular physical exercise improve gut dysbiosis and reduce disease risk.
33724115	6	52	theme	obesity	752:758	arg1	risk					699:702	the risk	695:702	the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD)	695:832	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	4	53	theme	protein	413:419	arg1	products					434:441	Several protein fermentation products	405:441	Several protein fermentation products	405:441	Several protein fermentation products, including indoles, phenols, polyamines, hydrogen sulfide (H2S), amines, and carnitine, are toxic.
33724115	2	54	theme	microbial	223:231	arg1	fermentation					233:244	microbial fermentation	223:244	microbial fermentation in the intestine	223:261	Compared with carbohydrates and fats, proteins are metabolized primarily by microbial fermentation in the intestine.
33724115	1	55	from	role	65:68	arg1	health					97:102	health	97:102	health	97:102	The role of the intestinal flora in health and disease has become a research hotspot.
33724115	1	55	from	role	65:68	arg1	disease					108:114	disease	108:114	disease	108:114	The role of the intestinal flora in health and disease has become a research hotspot.
33724115	8	56	theme	using	1086:1090	arg1	HPDs					1092:1095	65 years old using HPDs	1073:1095	65 years old using HPDs	1073:1095	However, meeting the protein requirements of individuals over 65 years old using HPDs is more challenging.
33724115	4	57	theme	Several	405:411	arg1	products					434:441	Several protein fermentation products	405:441	Several protein fermentation products	405:441	Several protein fermentation products, including indoles, phenols, polyamines, hydrogen sulfide (H2S), amines, and carnitine, are toxic.
33724115	6	58	theme	Long-term	671:679	arg1	HPDs					681:684	Long-term HPDs	671:684	Long-term HPDs	671:684	Long-term HPDs increase the risk of intestinal diseases, type 2 diabetes (T2DM), obesity, central nervous system (CNS) diseases, and cardiovascular diseases (CVD) by producing toxic metabolites in the colon, including amines, H2S, and ammonia.
33724115	3	59	theme	metabolites	316:326	arg1	production					268:277	The production	264:277	The production of protein fermentation products and metabolites	264:326	The production of protein fermentation products and metabolites depends on the composition, diversity, and metabolism of the gut microbiota.
33724115	0	60	from	diet	20:23	arg1	health					53:58	human health	47:58	human health	47:58	High animal protein diet and gut microbiota in human health.
33724115	8	61	theme	65 years	1073:1080	arg1	HPDs					1092:1095	65 years old using HPDs	1073:1095	65 years old using HPDs	1073:1095	However, meeting the protein requirements of individuals over 65 years old using HPDs is more challenging.
35164571	9	0	theme	reaction	1620:1627	arg1	UDP-MurNAc-Ser					1560:1573	UDP-MurNAc-Ser	1560:1573	UDP-MurNAc-Ser	1560:1573	YfiH selectively hydrolyzes UDP-MurNAc-Ser, an incorrect by-product of the biosynthesis reaction, to ensure that only the correct PG precursor, UDP-MurNAc-Ala, is incorporated.
35164571	9	0	theme	reaction	1620:1627	arg1	by-product					1589:1598	an incorrect by-product	1576:1598	an incorrect by-product of the biosynthesis reaction	1576:1627	YfiH selectively hydrolyzes UDP-MurNAc-Ser, an incorrect by-product of the biosynthesis reaction, to ensure that only the correct PG precursor, UDP-MurNAc-Ala, is incorporated.
35164571	8	1	theme	unknown	1382:1388	arg1	activity					1390:1397	a previously unknown activity	1369:1397	a previously unknown activity specific for the UDP-MurNAc-monopeptide, one of the nucleotide precursors from the cytoplasmic steps of the PG biosynthesis pathway	1369:1529	Through X-ray crystallographic and biochemical analyses, we demonstrate that YfiH is a hydrolase with a previously unknown activity specific for the UDP-MurNAc-monopeptide, one of the nucleotide precursors from the cytoplasmic steps of the PG biosynthesis pathway.
35164571	1	2	theme	disaccharide	129:140	arg1	GlcNAc					163:168	GlcNAc	163:168	GlcNAc	163:168	Bacterial cells are encased in peptidoglycan (PG), a polymer of disaccharide N-acetylglucosamine (GlcNAc) and N-acetyl-muramic acid (MurNAc) cross-linked by peptide stems.
35164571	1	2	theme	disaccharide	129:140	arg1	N-acetylglucosamine					142:160	disaccharide N-acetylglucosamine	129:160	disaccharide N-acetylglucosamine (GlcNAc)	129:169	Bacterial cells are encased in peptidoglycan (PG), a polymer of disaccharide N-acetylglucosamine (GlcNAc) and N-acetyl-muramic acid (MurNAc) cross-linked by peptide stems.
35164571	5	3	from	mechanism	939:947	arg1	steps					968:972	the cytoplasmic steps	952:972	the cytoplasmic steps of peptidoglycan biosynthesis	952:1002	This work reveals an editing mechanism in the cytoplasmic steps of peptidoglycan biosynthesis.
35164571	7	4	theme	YfiH	1172:1175	arg1	activity					1160:1167	the activity	1156:1167	the activity of YfiH	1156:1175	However, the activity of YfiH has not been deciphered, and the editing mechanism involving YfiH has remained a mystery.
35164571	4	5	theme	acid	795:798	arg1	incorporation					760:772	the incorporation	756:772	the incorporation of the correct amino acid to the nucleotide precursors	756:827	Here, we report the crystal structures of substrate-bound and product-bound YfiH, showing that YfiH is a cytoplasmic amidase that controls the incorporation of the correct amino acid to the nucleotide precursors by preferentially cleaving the nucleotide precursor by-product UDP-MurNAc-l-Ser.
35164571	2	6	theme	acid	325:328	arg1	unique					360:365	unique	360:365	unique	360:365	PG is synthesized in the cytoplasm as UDP-MurNAc-peptide precursors, of which the amino acid composition of the peptide is unique, with l-Ala added at the first position in most bacteria but with l-Ser or Gly in some bacteria.
35164571	2	6	theme	acid	325:328	arg1	composition					330:340	the amino acid composition	315:340	the amino acid composition of the peptide	315:355	PG is synthesized in the cytoplasm as UDP-MurNAc-peptide precursors, of which the amino acid composition of the peptide is unique, with l-Ala added at the first position in most bacteria but with l-Ser or Gly in some bacteria.
35164571	8	7	theme	precursors	1462:1471	arg1	precursors					1462:1471	the nucleotide precursors	1447:1471	the nucleotide precursors from the cytoplasmic steps of the PG biosynthesis pathway	1447:1529	Through X-ray crystallographic and biochemical analyses, we demonstrate that YfiH is a hydrolase with a previously unknown activity specific for the UDP-MurNAc-monopeptide, one of the nucleotide precursors from the cytoplasmic steps of the PG biosynthesis pathway.
35164571	8	7	theme	precursors	1462:1471	arg1	one					1440:1442	one	1440:1442	one	1440:1442	Through X-ray crystallographic and biochemical analyses, we demonstrate that YfiH is a hydrolase with a previously unknown activity specific for the UDP-MurNAc-monopeptide, one of the nucleotide precursors from the cytoplasmic steps of the PG biosynthesis pathway.
35164571	8	7	theme	precursors	1462:1471	arg1	UDP-MurNAc-monopeptide					1416:1437	the UDP-MurNAc-monopeptide	1412:1437	the UDP-MurNAc-monopeptide	1412:1437	Through X-ray crystallographic and biochemical analyses, we demonstrate that YfiH is a hydrolase with a previously unknown activity specific for the UDP-MurNAc-monopeptide, one of the nucleotide precursors from the cytoplasmic steps of the PG biosynthesis pathway.
35164571	10	8	theme	biosynthesis	1809:1820	arg1	steps					1797:1801	the cytoplasmic steps	1781:1801	the cytoplasmic steps of PG biosynthesis	1781:1820	Therefore, this work reveals coupled synthetic and editing reactions in the cytoplasmic steps of PG biosynthesis.
35164571	2	9	with	unique	360:365	arg1	Gly					442:444	Gly	442:444	Gly in some bacteria	442:461	PG is synthesized in the cytoplasm as UDP-MurNAc-peptide precursors, of which the amino acid composition of the peptide is unique, with l-Ala added at the first position in most bacteria but with l-Ser or Gly in some bacteria.
35164571	2	9	with	unique	360:365	arg1	l-Ala					373:377	l-Ala	373:377	l-Ala added at the first position in most bacteria	373:422	PG is synthesized in the cytoplasm as UDP-MurNAc-peptide precursors, of which the amino acid composition of the peptide is unique, with l-Ala added at the first position in most bacteria but with l-Ser or Gly in some bacteria.
35164571	2	9	with	unique	360:365	arg1	l-Ser					433:437	l-Ser	433:437	l-Ser	433:437	PG is synthesized in the cytoplasm as UDP-MurNAc-peptide precursors, of which the amino acid composition of the peptide is unique, with l-Ala added at the first position in most bacteria but with l-Ser or Gly in some bacteria.
35164571	4	10	theme	nucleotide	807:816	arg1	precursors					818:827	the nucleotide precursors	803:827	the nucleotide precursors	803:827	Here, we report the crystal structures of substrate-bound and product-bound YfiH, showing that YfiH is a cytoplasmic amidase that controls the incorporation of the correct amino acid to the nucleotide precursors by preferentially cleaving the nucleotide precursor by-product UDP-MurNAc-l-Ser.
35164571	3	11	theme	PG-editing	474:483	arg1	factor					485:490	a PG-editing factor	472:490	a PG-editing factor whose absence causes misincorporation of l-Ser instead of l-Ala into peptide stems	472:573	YfiH is a PG-editing factor whose absence causes misincorporation of l-Ser instead of l-Ala into peptide stems, but its mechanistic function is unknown.
35164571	3	11	theme	PG-editing	474:483	arg1	YfiH					464:467	YfiH	464:467	YfiH	464:467	YfiH is a PG-editing factor whose absence causes misincorporation of l-Ser instead of l-Ala into peptide stems, but its mechanistic function is unknown.
35164571	4	12	theme	product-bound	679:691	arg1	YfiH					693:696	substrate-bound and product-bound YfiH	659:696	substrate-bound and product-bound YfiH	659:696	Here, we report the crystal structures of substrate-bound and product-bound YfiH, showing that YfiH is a cytoplasmic amidase that controls the incorporation of the correct amino acid to the nucleotide precursors by preferentially cleaving the nucleotide precursor by-product UDP-MurNAc-l-Ser.
35164571	6	13	theme	compositions	1112:1123	arg1	maintenance					1077:1087	the maintenance	1073:1087	the maintenance of specific amino acid compositions of the stem peptides	1073:1144	IMPORTANCE YfiH is a peptidoglycan (PG)-editing factor required for the maintenance of specific amino acid compositions of the stem peptides.
35164571	6	14	theme	peptides	1137:1144	arg1	compositions					1112:1123	specific amino acid compositions	1092:1123	specific amino acid compositions of the stem peptides	1092:1144	IMPORTANCE YfiH is a peptidoglycan (PG)-editing factor required for the maintenance of specific amino acid compositions of the stem peptides.
35164571	8	15	theme	crystallographic	1281:1296	arg1	analyses					1314:1321	X-ray crystallographic and biochemical analyses	1275:1321	X-ray crystallographic and biochemical analyses	1275:1321	Through X-ray crystallographic and biochemical analyses, we demonstrate that YfiH is a hydrolase with a previously unknown activity specific for the UDP-MurNAc-monopeptide, one of the nucleotide precursors from the cytoplasmic steps of the PG biosynthesis pathway.
35164571	1	16	theme	Bacterial	65:73	arg1	cells					75:79	Bacterial cells	65:79	Bacterial cells	65:79	Bacterial cells are encased in peptidoglycan (PG), a polymer of disaccharide N-acetylglucosamine (GlcNAc) and N-acetyl-muramic acid (MurNAc) cross-linked by peptide stems.
35164571	4	17	theme	crystal	637:643	arg1	structures					645:654	the crystal structures	633:654	the crystal structures of substrate-bound and product-bound YfiH	633:696	Here, we report the crystal structures of substrate-bound and product-bound YfiH, showing that YfiH is a cytoplasmic amidase that controls the incorporation of the correct amino acid to the nucleotide precursors by preferentially cleaving the nucleotide precursor by-product UDP-MurNAc-l-Ser.
35164571	8	18	theme	cytoplasmic	1482:1492	arg1	steps					1494:1498	the cytoplasmic steps	1478:1498	the cytoplasmic steps of the PG biosynthesis pathway	1478:1529	Through X-ray crystallographic and biochemical analyses, we demonstrate that YfiH is a hydrolase with a previously unknown activity specific for the UDP-MurNAc-monopeptide, one of the nucleotide precursors from the cytoplasmic steps of the PG biosynthesis pathway.
35164571	5	19	theme	editing	931:937	arg1	mechanism					939:947	an editing mechanism	928:947	an editing mechanism in the cytoplasmic steps of peptidoglycan biosynthesis	928:1002	This work reveals an editing mechanism in the cytoplasmic steps of peptidoglycan biosynthesis.
35164571	2	20	theme	most	410:413	arg1	bacteria					415:422	most bacteria	410:422	most bacteria	410:422	PG is synthesized in the cytoplasm as UDP-MurNAc-peptide precursors, of which the amino acid composition of the peptide is unique, with l-Ala added at the first position in most bacteria but with l-Ser or Gly in some bacteria.
35164571	8	21	from	steps	1494:1498	arg1	precursors					1462:1471	the nucleotide precursors	1447:1471	the nucleotide precursors from the cytoplasmic steps of the PG biosynthesis pathway	1447:1529	Through X-ray crystallographic and biochemical analyses, we demonstrate that YfiH is a hydrolase with a previously unknown activity specific for the UDP-MurNAc-monopeptide, one of the nucleotide precursors from the cytoplasmic steps of the PG biosynthesis pathway.
35164571	6	22	theme	acid	1107:1110	arg1	compositions					1112:1123	specific amino acid compositions	1092:1123	specific amino acid compositions of the stem peptides	1092:1144	IMPORTANCE YfiH is a peptidoglycan (PG)-editing factor required for the maintenance of specific amino acid compositions of the stem peptides.
35164571	8	23	theme	biochemical	1302:1312	arg1	analyses					1314:1321	X-ray crystallographic and biochemical analyses	1275:1321	X-ray crystallographic and biochemical analyses	1275:1321	Through X-ray crystallographic and biochemical analyses, we demonstrate that YfiH is a hydrolase with a previously unknown activity specific for the UDP-MurNAc-monopeptide, one of the nucleotide precursors from the cytoplasmic steps of the PG biosynthesis pathway.
35164571	6	24	theme	-editing	1044:1051	arg1	IMPORTANCE					1005:1014	IMPORTANCE YfiH	1005:1019	IMPORTANCE YfiH	1005:1019	IMPORTANCE YfiH is a peptidoglycan (PG)-editing factor required for the maintenance of specific amino acid compositions of the stem peptides.
35164571	6	24	theme	-editing	1044:1051	arg1	factor					1053:1058	a peptidoglycan (PG)-editing factor	1024:1058	a peptidoglycan (PG)-editing factor required for the maintenance of specific amino acid compositions of the stem peptides	1024:1144	IMPORTANCE YfiH is a peptidoglycan (PG)-editing factor required for the maintenance of specific amino acid compositions of the stem peptides.
35164571	3	25	theme	mechanistic	584:594	arg1	function					596:603	its mechanistic function	580:603	its mechanistic function	580:603	YfiH is a PG-editing factor whose absence causes misincorporation of l-Ser instead of l-Ala into peptide stems, but its mechanistic function is unknown.
35164571	4	26	theme	amino	789:793	arg1	acid					795:798	the correct amino acid	777:798	the correct amino acid	777:798	Here, we report the crystal structures of substrate-bound and product-bound YfiH, showing that YfiH is a cytoplasmic amidase that controls the incorporation of the correct amino acid to the nucleotide precursors by preferentially cleaving the nucleotide precursor by-product UDP-MurNAc-l-Ser.
35164571	8	27	with	hydrolase	1354:1362	arg1	activity					1390:1397	a previously unknown activity	1369:1397	a previously unknown activity specific for the UDP-MurNAc-monopeptide, one of the nucleotide precursors from the cytoplasmic steps of the PG biosynthesis pathway	1369:1529	Through X-ray crystallographic and biochemical analyses, we demonstrate that YfiH is a hydrolase with a previously unknown activity specific for the UDP-MurNAc-monopeptide, one of the nucleotide precursors from the cytoplasmic steps of the PG biosynthesis pathway.
35164571	0	28	theme	Structural	0:9	arg1	Basis					11:15	Structural Basis	0:15	Structural Basis for the Peptidoglycan-Editing Activity of YfiH	0:62	Structural Basis for the Peptidoglycan-Editing Activity of YfiH.
35164571	8	29	theme	PG	1507:1508	arg1	pathway					1523:1529	the PG biosynthesis pathway	1503:1529	the PG biosynthesis pathway	1503:1529	Through X-ray crystallographic and biochemical analyses, we demonstrate that YfiH is a hydrolase with a previously unknown activity specific for the UDP-MurNAc-monopeptide, one of the nucleotide precursors from the cytoplasmic steps of the PG biosynthesis pathway.
35164571	4	30	theme	correct	781:787	arg1	acid					795:798	the correct amino acid	777:798	the correct amino acid	777:798	Here, we report the crystal structures of substrate-bound and product-bound YfiH, showing that YfiH is a cytoplasmic amidase that controls the incorporation of the correct amino acid to the nucleotide precursors by preferentially cleaving the nucleotide precursor by-product UDP-MurNAc-l-Ser.
35164571	2	31	theme	precursors	294:303	arg1	unique					360:365	unique	360:365	unique	360:365	PG is synthesized in the cytoplasm as UDP-MurNAc-peptide precursors, of which the amino acid composition of the peptide is unique, with l-Ala added at the first position in most bacteria but with l-Ser or Gly in some bacteria.
35164571	2	31	theme	precursors	294:303	arg1	composition					330:340	the amino acid composition	315:340	the amino acid composition of the peptide	315:355	PG is synthesized in the cytoplasm as UDP-MurNAc-peptide precursors, of which the amino acid composition of the peptide is unique, with l-Ala added at the first position in most bacteria but with l-Ser or Gly in some bacteria.
35164571	2	32	theme	peptide	349:355	arg1	unique					360:365	unique	360:365	unique	360:365	PG is synthesized in the cytoplasm as UDP-MurNAc-peptide precursors, of which the amino acid composition of the peptide is unique, with l-Ala added at the first position in most bacteria but with l-Ser or Gly in some bacteria.
35164571	2	32	theme	peptide	349:355	arg1	composition					330:340	the amino acid composition	315:340	the amino acid composition of the peptide	315:355	PG is synthesized in the cytoplasm as UDP-MurNAc-peptide precursors, of which the amino acid composition of the peptide is unique, with l-Ala added at the first position in most bacteria but with l-Ser or Gly in some bacteria.
35164571	2	33	theme	first	392:396	arg1	position					398:405	the first position	388:405	the first position in most bacteria	388:422	PG is synthesized in the cytoplasm as UDP-MurNAc-peptide precursors, of which the amino acid composition of the peptide is unique, with l-Ala added at the first position in most bacteria but with l-Ser or Gly in some bacteria.
35164571	5	34	theme	cytoplasmic	956:966	arg1	steps					968:972	the cytoplasmic steps	952:972	the cytoplasmic steps of peptidoglycan biosynthesis	952:1002	This work reveals an editing mechanism in the cytoplasmic steps of peptidoglycan biosynthesis.
35164571	4	35	theme	nucleotide	860:869	arg1	UDP-MurNAc-l-Ser					892:907	the nucleotide precursor by-product UDP-MurNAc-l-Ser	856:907	the nucleotide precursor by-product UDP-MurNAc-l-Ser	856:907	Here, we report the crystal structures of substrate-bound and product-bound YfiH, showing that YfiH is a cytoplasmic amidase that controls the incorporation of the correct amino acid to the nucleotide precursors by preferentially cleaving the nucleotide precursor by-product UDP-MurNAc-l-Ser.
35164571	9	36	theme	correct	1654:1660	arg1	UDP-MurNAc-Ala					1676:1689	UDP-MurNAc-Ala	1676:1689	UDP-MurNAc-Ala	1676:1689	YfiH selectively hydrolyzes UDP-MurNAc-Ser, an incorrect by-product of the biosynthesis reaction, to ensure that only the correct PG precursor, UDP-MurNAc-Ala, is incorporated.
35164571	9	36	theme	correct	1654:1660	arg1	precursor					1665:1673	only the correct PG precursor	1645:1673	only the correct PG precursor	1645:1673	YfiH selectively hydrolyzes UDP-MurNAc-Ser, an incorrect by-product of the biosynthesis reaction, to ensure that only the correct PG precursor, UDP-MurNAc-Ala, is incorporated.
35164571	2	37	theme	UDP-MurNAc-peptide	275:292	arg1	PG					237:238	PG	237:238	PG	237:238	PG is synthesized in the cytoplasm as UDP-MurNAc-peptide precursors, of which the amino acid composition of the peptide is unique, with l-Ala added at the first position in most bacteria but with l-Ser or Gly in some bacteria.
35164571	2	37	theme	UDP-MurNAc-peptide	275:292	arg1	precursors					294:303	UDP-MurNAc-peptide precursors	275:303	UDP-MurNAc-peptide precursors	275:303	PG is synthesized in the cytoplasm as UDP-MurNAc-peptide precursors, of which the amino acid composition of the peptide is unique, with l-Ala added at the first position in most bacteria but with l-Ser or Gly in some bacteria.
35164571	1	38	theme	N-acetyl-muramic	175:190	arg1	acid					192:195	N-acetyl-muramic acid	175:195	N-acetyl-muramic acid (MurNAc)	175:204	Bacterial cells are encased in peptidoglycan (PG), a polymer of disaccharide N-acetylglucosamine (GlcNAc) and N-acetyl-muramic acid (MurNAc) cross-linked by peptide stems.
35164571	1	38	theme	N-acetyl-muramic	175:190	arg1	MurNAc					198:203	MurNAc	198:203	MurNAc	198:203	Bacterial cells are encased in peptidoglycan (PG), a polymer of disaccharide N-acetylglucosamine (GlcNAc) and N-acetyl-muramic acid (MurNAc) cross-linked by peptide stems.
35164571	4	39	theme	substrate-bound	659:673	arg1	YfiH					693:696	substrate-bound and product-bound YfiH	659:696	substrate-bound and product-bound YfiH	659:696	Here, we report the crystal structures of substrate-bound and product-bound YfiH, showing that YfiH is a cytoplasmic amidase that controls the incorporation of the correct amino acid to the nucleotide precursors by preferentially cleaving the nucleotide precursor by-product UDP-MurNAc-l-Ser.
35164571	8	40	theme	pathway	1523:1529	arg1	steps					1494:1498	the cytoplasmic steps	1478:1498	the cytoplasmic steps of the PG biosynthesis pathway	1478:1529	Through X-ray crystallographic and biochemical analyses, we demonstrate that YfiH is a hydrolase with a previously unknown activity specific for the UDP-MurNAc-monopeptide, one of the nucleotide precursors from the cytoplasmic steps of the PG biosynthesis pathway.
35164571	6	41	theme	stem	1132:1135	arg1	peptides					1137:1144	the stem peptides	1128:1144	the stem peptides	1128:1144	IMPORTANCE YfiH is a peptidoglycan (PG)-editing factor required for the maintenance of specific amino acid compositions of the stem peptides.
35164571	8	42	theme	specific	1399:1406	arg1	activity					1390:1397	a previously unknown activity	1369:1397	a previously unknown activity specific for the UDP-MurNAc-monopeptide, one of the nucleotide precursors from the cytoplasmic steps of the PG biosynthesis pathway	1369:1529	Through X-ray crystallographic and biochemical analyses, we demonstrate that YfiH is a hydrolase with a previously unknown activity specific for the UDP-MurNAc-monopeptide, one of the nucleotide precursors from the cytoplasmic steps of the PG biosynthesis pathway.
35164571	1	43	theme	N-acetylglucosamine	142:160	arg1	peptidoglycan					96:108	peptidoglycan	96:108	peptidoglycan (PG)	96:113	Bacterial cells are encased in peptidoglycan (PG), a polymer of disaccharide N-acetylglucosamine (GlcNAc) and N-acetyl-muramic acid (MurNAc) cross-linked by peptide stems.
35164571	1	43	theme	N-acetylglucosamine	142:160	arg1	polymer					118:124	a polymer	116:124	a polymer of disaccharide N-acetylglucosamine (GlcNAc) and N-acetyl-muramic acid (MurNAc) cross-linked by peptide stems	116:234	Bacterial cells are encased in peptidoglycan (PG), a polymer of disaccharide N-acetylglucosamine (GlcNAc) and N-acetyl-muramic acid (MurNAc) cross-linked by peptide stems.
35164571	1	44	theme	acid	192:195	arg1	peptidoglycan					96:108	peptidoglycan	96:108	peptidoglycan (PG)	96:113	Bacterial cells are encased in peptidoglycan (PG), a polymer of disaccharide N-acetylglucosamine (GlcNAc) and N-acetyl-muramic acid (MurNAc) cross-linked by peptide stems.
35164571	1	44	theme	acid	192:195	arg1	polymer					118:124	a polymer	116:124	a polymer of disaccharide N-acetylglucosamine (GlcNAc) and N-acetyl-muramic acid (MurNAc) cross-linked by peptide stems	116:234	Bacterial cells are encased in peptidoglycan (PG), a polymer of disaccharide N-acetylglucosamine (GlcNAc) and N-acetyl-muramic acid (MurNAc) cross-linked by peptide stems.
35164571	8	45	theme	biosynthesis	1510:1521	arg1	pathway					1523:1529	the PG biosynthesis pathway	1503:1529	the PG biosynthesis pathway	1503:1529	Through X-ray crystallographic and biochemical analyses, we demonstrate that YfiH is a hydrolase with a previously unknown activity specific for the UDP-MurNAc-monopeptide, one of the nucleotide precursors from the cytoplasmic steps of the PG biosynthesis pathway.
35164571	4	46	theme	by-product	881:890	arg1	UDP-MurNAc-l-Ser					892:907	the nucleotide precursor by-product UDP-MurNAc-l-Ser	856:907	the nucleotide precursor by-product UDP-MurNAc-l-Ser	856:907	Here, we report the crystal structures of substrate-bound and product-bound YfiH, showing that YfiH is a cytoplasmic amidase that controls the incorporation of the correct amino acid to the nucleotide precursors by preferentially cleaving the nucleotide precursor by-product UDP-MurNAc-l-Ser.
35164571	2	47	from	l-Ser	433:437	arg1	bacteria					454:461	some bacteria	449:461	some bacteria	449:461	PG is synthesized in the cytoplasm as UDP-MurNAc-peptide precursors, of which the amino acid composition of the peptide is unique, with l-Ala added at the first position in most bacteria but with l-Ser or Gly in some bacteria.
35164571	10	48	theme	editing	1760:1766	arg1	reactions					1768:1776	coupled synthetic and editing reactions	1738:1776	coupled synthetic and editing reactions	1738:1776	Therefore, this work reveals coupled synthetic and editing reactions in the cytoplasmic steps of PG biosynthesis.
35164571	0	49	theme	Peptidoglycan-Editing	25:45	arg1	Activity					47:54	the Peptidoglycan-Editing Activity	21:54	the Peptidoglycan-Editing Activity of YfiH	21:62	Structural Basis for the Peptidoglycan-Editing Activity of YfiH.
35164571	4	50	theme	precursor	871:879	arg1	UDP-MurNAc-l-Ser					892:907	the nucleotide precursor by-product UDP-MurNAc-l-Ser	856:907	the nucleotide precursor by-product UDP-MurNAc-l-Ser	856:907	Here, we report the crystal structures of substrate-bound and product-bound YfiH, showing that YfiH is a cytoplasmic amidase that controls the incorporation of the correct amino acid to the nucleotide precursors by preferentially cleaving the nucleotide precursor by-product UDP-MurNAc-l-Ser.
35164571	5	51	theme	peptidoglycan	977:989	arg1	biosynthesis					991:1002	peptidoglycan biosynthesis	977:1002	peptidoglycan biosynthesis	977:1002	This work reveals an editing mechanism in the cytoplasmic steps of peptidoglycan biosynthesis.
35164571	0	52	theme	YfiH	59:62	arg1	Activity					47:54	the Peptidoglycan-Editing Activity	21:54	the Peptidoglycan-Editing Activity of YfiH	21:62	Structural Basis for the Peptidoglycan-Editing Activity of YfiH.
35164571	2	53	from	Gly	442:444	arg1	bacteria					454:461	some bacteria	449:461	some bacteria	449:461	PG is synthesized in the cytoplasm as UDP-MurNAc-peptide precursors, of which the amino acid composition of the peptide is unique, with l-Ala added at the first position in most bacteria but with l-Ser or Gly in some bacteria.
35164571	5	54	theme	biosynthesis	991:1002	arg1	steps					968:972	the cytoplasmic steps	952:972	the cytoplasmic steps of peptidoglycan biosynthesis	952:1002	This work reveals an editing mechanism in the cytoplasmic steps of peptidoglycan biosynthesis.
35164571	2	55	theme	amino	319:323	arg1	unique					360:365	unique	360:365	unique	360:365	PG is synthesized in the cytoplasm as UDP-MurNAc-peptide precursors, of which the amino acid composition of the peptide is unique, with l-Ala added at the first position in most bacteria but with l-Ser or Gly in some bacteria.
35164571	2	55	theme	amino	319:323	arg1	composition					330:340	the amino acid composition	315:340	the amino acid composition of the peptide	315:355	PG is synthesized in the cytoplasm as UDP-MurNAc-peptide precursors, of which the amino acid composition of the peptide is unique, with l-Ala added at the first position in most bacteria but with l-Ser or Gly in some bacteria.
35164571	2	56	from	position	398:405	arg1	bacteria					415:422	most bacteria	410:422	most bacteria	410:422	PG is synthesized in the cytoplasm as UDP-MurNAc-peptide precursors, of which the amino acid composition of the peptide is unique, with l-Ala added at the first position in most bacteria but with l-Ser or Gly in some bacteria.
35164571	8	57	theme	nucleotide	1451:1460	arg1	precursors					1462:1471	the nucleotide precursors	1447:1471	the nucleotide precursors from the cytoplasmic steps of the PG biosynthesis pathway	1447:1529	Through X-ray crystallographic and biochemical analyses, we demonstrate that YfiH is a hydrolase with a previously unknown activity specific for the UDP-MurNAc-monopeptide, one of the nucleotide precursors from the cytoplasmic steps of the PG biosynthesis pathway.
35164571	3	58	theme	l-Ser	533:537	arg1	misincorporation					513:528	misincorporation	513:528	misincorporation of l-Ser instead of l-Ala into peptide stems	513:573	YfiH is a PG-editing factor whose absence causes misincorporation of l-Ser instead of l-Ala into peptide stems, but its mechanistic function is unknown.
35164571	10	59	theme	synthetic	1746:1754	arg1	reactions					1768:1776	coupled synthetic and editing reactions	1738:1776	coupled synthetic and editing reactions	1738:1776	Therefore, this work reveals coupled synthetic and editing reactions in the cytoplasmic steps of PG biosynthesis.
35164571	9	60	theme	PG	1662:1663	arg1	UDP-MurNAc-Ala					1676:1689	UDP-MurNAc-Ala	1676:1689	UDP-MurNAc-Ala	1676:1689	YfiH selectively hydrolyzes UDP-MurNAc-Ser, an incorrect by-product of the biosynthesis reaction, to ensure that only the correct PG precursor, UDP-MurNAc-Ala, is incorporated.
35164571	9	60	theme	PG	1662:1663	arg1	precursor					1665:1673	only the correct PG precursor	1645:1673	only the correct PG precursor	1645:1673	YfiH selectively hydrolyzes UDP-MurNAc-Ser, an incorrect by-product of the biosynthesis reaction, to ensure that only the correct PG precursor, UDP-MurNAc-Ala, is incorporated.
35164571	10	61	theme	PG	1806:1807	arg1	biosynthesis					1809:1820	PG biosynthesis	1806:1820	PG biosynthesis	1806:1820	Therefore, this work reveals coupled synthetic and editing reactions in the cytoplasmic steps of PG biosynthesis.
35164571	3	62	theme	peptide	561:567	arg1	stems					569:573	peptide stems	561:573	peptide stems	561:573	YfiH is a PG-editing factor whose absence causes misincorporation of l-Ser instead of l-Ala into peptide stems, but its mechanistic function is unknown.
35164571	9	63	theme	biosynthesis	1607:1618	arg1	reaction					1620:1627	the biosynthesis reaction	1603:1627	the biosynthesis reaction	1603:1627	YfiH selectively hydrolyzes UDP-MurNAc-Ser, an incorrect by-product of the biosynthesis reaction, to ensure that only the correct PG precursor, UDP-MurNAc-Ala, is incorporated.
35164571	4	64	theme	YfiH	693:696	arg1	structures					645:654	the crystal structures	633:654	the crystal structures of substrate-bound and product-bound YfiH	633:696	Here, we report the crystal structures of substrate-bound and product-bound YfiH, showing that YfiH is a cytoplasmic amidase that controls the incorporation of the correct amino acid to the nucleotide precursors by preferentially cleaving the nucleotide precursor by-product UDP-MurNAc-l-Ser.
35164571	7	65	theme	editing	1210:1216	arg1	mechanism					1218:1226	the editing mechanism	1206:1226	the editing mechanism involving YfiH	1206:1241	However, the activity of YfiH has not been deciphered, and the editing mechanism involving YfiH has remained a mystery.
35164571	6	66	theme	specific	1092:1099	arg1	compositions					1112:1123	specific amino acid compositions	1092:1123	specific amino acid compositions of the stem peptides	1092:1144	IMPORTANCE YfiH is a peptidoglycan (PG)-editing factor required for the maintenance of specific amino acid compositions of the stem peptides.
35164571	10	67	theme	coupled	1738:1744	arg1	reactions					1768:1776	coupled synthetic and editing reactions	1738:1776	coupled synthetic and editing reactions	1738:1776	Therefore, this work reveals coupled synthetic and editing reactions in the cytoplasmic steps of PG biosynthesis.
35164571	3	68	theme	l-Ala	550:554	arg1	misincorporation					513:528	misincorporation	513:528	misincorporation of l-Ser instead of l-Ala into peptide stems	513:573	YfiH is a PG-editing factor whose absence causes misincorporation of l-Ser instead of l-Ala into peptide stems, but its mechanistic function is unknown.
35164571	6	69	theme	amino	1101:1105	arg1	compositions					1112:1123	specific amino acid compositions	1092:1123	specific amino acid compositions of the stem peptides	1092:1144	IMPORTANCE YfiH is a peptidoglycan (PG)-editing factor required for the maintenance of specific amino acid compositions of the stem peptides.
35164571	4	70	theme	cytoplasmic	722:732	arg1	amidase					734:740	a cytoplasmic amidase	720:740	a cytoplasmic amidase that controls the incorporation of the correct amino acid to the nucleotide precursors by preferentially cleaving the nucleotide precursor by-product UDP-MurNAc-l-Ser	720:907	Here, we report the crystal structures of substrate-bound and product-bound YfiH, showing that YfiH is a cytoplasmic amidase that controls the incorporation of the correct amino acid to the nucleotide precursors by preferentially cleaving the nucleotide precursor by-product UDP-MurNAc-l-Ser.
35164571	4	70	theme	cytoplasmic	722:732	arg1	YfiH					712:715	YfiH	712:715	YfiH	712:715	Here, we report the crystal structures of substrate-bound and product-bound YfiH, showing that YfiH is a cytoplasmic amidase that controls the incorporation of the correct amino acid to the nucleotide precursors by preferentially cleaving the nucleotide precursor by-product UDP-MurNAc-l-Ser.
35164571	10	71	theme	cytoplasmic	1785:1795	arg1	steps					1797:1801	the cytoplasmic steps	1781:1801	the cytoplasmic steps of PG biosynthesis	1781:1820	Therefore, this work reveals coupled synthetic and editing reactions in the cytoplasmic steps of PG biosynthesis.
35164571	9	72	theme	incorrect	1579:1587	arg1	UDP-MurNAc-Ser					1560:1573	UDP-MurNAc-Ser	1560:1573	UDP-MurNAc-Ser	1560:1573	YfiH selectively hydrolyzes UDP-MurNAc-Ser, an incorrect by-product of the biosynthesis reaction, to ensure that only the correct PG precursor, UDP-MurNAc-Ala, is incorporated.
35164571	9	72	theme	incorrect	1579:1587	arg1	by-product					1589:1598	an incorrect by-product	1576:1598	an incorrect by-product of the biosynthesis reaction	1576:1627	YfiH selectively hydrolyzes UDP-MurNAc-Ser, an incorrect by-product of the biosynthesis reaction, to ensure that only the correct PG precursor, UDP-MurNAc-Ala, is incorporated.
35164571	1	73	theme	peptide	222:228	arg1	stems					230:234	peptide stems	222:234	peptide stems	222:234	Bacterial cells are encased in peptidoglycan (PG), a polymer of disaccharide N-acetylglucosamine (GlcNAc) and N-acetyl-muramic acid (MurNAc) cross-linked by peptide stems.
34678541	4	0	theme	fermentation	943:954	arg1	time					956:959	fermentation time	943:959	fermentation time	943:959	Amplification of the organism's functioning resulted in an enhancement of sugar productivity and yield accompanied by a significant reduction in fermentation time.
34678541	3	1	theme	Surface	754:760	arg1	Methodology					762:772	Response Surface Methodology	745:772	Response Surface Methodology	745:772	Thus, the present work adopted a dual phase statistical optimization approach for the biological pre-treatment of sugarcane bagasse, with Pycnoporus coccineus MScMS1, using Taguchi Orthogonal Array, in conjunction with Response Surface Methodology, to address this issue.
34678541	3	2	theme	dual	559:562	arg1	approach					595:602	a dual phase statistical optimization approach	557:602	a dual phase statistical optimization approach	557:602	Thus, the present work adopted a dual phase statistical optimization approach for the biological pre-treatment of sugarcane bagasse, with Pycnoporus coccineus MScMS1, using Taguchi Orthogonal Array, in conjunction with Response Surface Methodology, to address this issue.
34678541	1	3	theme	ligninolytic	301:312	arg1	enzymes					314:320	their ligninolytic enzymes	295:320	their ligninolytic enzymes	295:320	Biological pre-treatment is the removal of recalcitrant lignin from lignocellulose through the action of lignin degrading organisms and/or their ligninolytic enzymes system.
34678541	7	4	dep	significant	1121:1131	arg1	p < 0.05					1134:1141	p < 0.05	1134:1141	p < 0.05	1134:1141	Substrate compositional analysis revealed significant (p < 0.05) reduction of lignin by 70% in the biologically pre-treated substrate, along with significantly (p < 0.05) higher quantities of water soluble components (35 ± 0.95 g) and cellulose content (33 ± 0.18 g), as compared to the untreated substrate.
34678541	2	5	theme	numerous	338:345	arg1	benefits					361:368	numerous environmental benefits	338:368	numerous environmental benefits	338:368	Despite numerous environmental benefits, biological pre-treatment has been side-lined due to its prolonged periods of fermentation, ascribed to the slow growth rate of lignin degrading organisms.
34678541	1	6	theme	system	322:327	arg1	action					251:256	the action	247:256	the action of lignin degrading organisms and/or their ligninolytic enzymes system	247:327	Biological pre-treatment is the removal of recalcitrant lignin from lignocellulose through the action of lignin degrading organisms and/or their ligninolytic enzymes system.
34678541	9	7	theme	65	1742:1743	arg1	%					1744:1744	%	1744:1744	%	1744:1744	Furthermore, Bacillus megaterium Ti3, a potent polyhydroxyalkanoates (PHA) producer, was grown on these sugar-rich hydrolysates and generated 0.58 g/L PHA in 24 h of fermentation accompanied by 0.88 g/L dry cell weight and 65% PHA accumulation.
34678541	7	8	theme	higher	1250:1255	arg1	quantities					1257:1266	significantly (p < 0.05) higher quantities	1225:1266	significantly (p < 0.05) higher quantities of water soluble components (35 ± 0.95 g) and cellulose content (33 ± 0.18 g)	1225:1344	Substrate compositional analysis revealed significant (p < 0.05) reduction of lignin by 70% in the biologically pre-treated substrate, along with significantly (p < 0.05) higher quantities of water soluble components (35 ± 0.95 g) and cellulose content (33 ± 0.18 g), as compared to the untreated substrate.
34678541	1	9	theme	lignin	212:217	arg1	pre-treatment					167:179	Biological pre-treatment	156:179	Biological pre-treatment	156:179	Biological pre-treatment is the removal of recalcitrant lignin from lignocellulose through the action of lignin degrading organisms and/or their ligninolytic enzymes system.
34678541	1	9	theme	lignin	212:217	arg1	removal					188:194	the removal	184:194	the removal of recalcitrant lignin from lignocellulose	184:237	Biological pre-treatment is the removal of recalcitrant lignin from lignocellulose through the action of lignin degrading organisms and/or their ligninolytic enzymes system.
34678541	9	10	theme	PHA	1746:1748	arg1	accumulation					1750:1761	65% PHA accumulation	1742:1761	65% PHA accumulation	1742:1761	Furthermore, Bacillus megaterium Ti3, a potent polyhydroxyalkanoates (PHA) producer, was grown on these sugar-rich hydrolysates and generated 0.58 g/L PHA in 24 h of fermentation accompanied by 0.88 g/L dry cell weight and 65% PHA accumulation.
34678541	2	11	theme	biological	371:380	arg1	pre-treatment					382:394	biological pre-treatment	371:394	biological pre-treatment	371:394	Despite numerous environmental benefits, biological pre-treatment has been side-lined due to its prolonged periods of fermentation, ascribed to the slow growth rate of lignin degrading organisms.
34678541	8	12	from	bagasse	1510:1516	arg1	hydrolysates					1467:1478	hydrolysates	1467:1478	hydrolysates from biologically pre-treated bagasse	1467:1516	Appreciable levels of xylose, arabinose, glucose and galactose were detected in hydrolysates from biologically pre-treated bagasse.
34678541	3	13	dep	Pycnoporus	664:673	arg1	coccineus					675:683	coccineus	675:683	coccineus	675:683	Thus, the present work adopted a dual phase statistical optimization approach for the biological pre-treatment of sugarcane bagasse, with Pycnoporus coccineus MScMS1, using Taguchi Orthogonal Array, in conjunction with Response Surface Methodology, to address this issue.
34678541	6	14	with	pre-treatment	1028:1040	arg1	productivity					1048:1059	productivity	1048:1059	productivity of 4.5 g/(L.day)	1048:1076	18 g/L within 4 days of pre-treatment, with productivity of 4.5 g/(L.day).
34678541	7	15	theme	lignin	1157:1162	arg1	reduction					1144:1152	significant (p < 0.05) reduction	1121:1152	significant (p < 0.05) reduction of lignin by 70% in the biologically pre-treated substrate	1121:1211	Substrate compositional analysis revealed significant (p < 0.05) reduction of lignin by 70% in the biologically pre-treated substrate, along with significantly (p < 0.05) higher quantities of water soluble components (35 ± 0.95 g) and cellulose content (33 ± 0.18 g), as compared to the untreated substrate.
34678541	11	16	theme	sugar-rich	1959:1968	arg1	hydrolysates					1970:1981	the resultant sugar-rich hydrolysates	1945:1981	the resultant sugar-rich hydrolysates	1945:1981	Thus, the present study was successful in optimizing the biological pre-treatment of sugarcane bagasse and utilizing the resultant sugar-rich hydrolysates, as inexpensive and renewable raw materials, for PHA production.
34678541	11	17	theme	biological	1885:1894	arg1	pre-treatment					1896:1908	the biological pre-treatment	1881:1908	the biological pre-treatment of sugarcane bagasse	1881:1929	Thus, the present study was successful in optimizing the biological pre-treatment of sugarcane bagasse and utilizing the resultant sugar-rich hydrolysates, as inexpensive and renewable raw materials, for PHA production.
34678541	3	18	theme	Pycnoporus	664:673	arg1	MScMS1					685:690	Pycnoporus coccineus MScMS1	664:690	Pycnoporus coccineus MScMS1	664:690	Thus, the present work adopted a dual phase statistical optimization approach for the biological pre-treatment of sugarcane bagasse, with Pycnoporus coccineus MScMS1, using Taguchi Orthogonal Array, in conjunction with Response Surface Methodology, to address this issue.
34678541	7	19	theme	compositional	1089:1101	arg1	analysis					1103:1110	Substrate compositional analysis	1079:1110	Substrate compositional analysis	1079:1110	Substrate compositional analysis revealed significant (p < 0.05) reduction of lignin by 70% in the biologically pre-treated substrate, along with significantly (p < 0.05) higher quantities of water soluble components (35 ± 0.95 g) and cellulose content (33 ± 0.18 g), as compared to the untreated substrate.
34678541	9	20	theme	polyhydroxyalkanoates	1566:1586	arg1	producer					1594:1601	a potent polyhydroxyalkanoates (PHA) producer	1557:1601	a potent polyhydroxyalkanoates (PHA) producer	1557:1601	Furthermore, Bacillus megaterium Ti3, a potent polyhydroxyalkanoates (PHA) producer, was grown on these sugar-rich hydrolysates and generated 0.58 g/L PHA in 24 h of fermentation accompanied by 0.88 g/L dry cell weight and 65% PHA accumulation.
34678541	9	20	theme	polyhydroxyalkanoates	1566:1586	arg1	Ti3					1552:1554	Bacillus megaterium Ti3	1532:1554	Bacillus megaterium Ti3	1532:1554	Furthermore, Bacillus megaterium Ti3, a potent polyhydroxyalkanoates (PHA) producer, was grown on these sugar-rich hydrolysates and generated 0.58 g/L PHA in 24 h of fermentation accompanied by 0.88 g/L dry cell weight and 65% PHA accumulation.
34678541	7	21	theme	significant	1121:1131	arg1	reduction					1144:1152	significant (p < 0.05) reduction	1121:1152	significant (p < 0.05) reduction of lignin by 70% in the biologically pre-treated substrate	1121:1211	Substrate compositional analysis revealed significant (p < 0.05) reduction of lignin by 70% in the biologically pre-treated substrate, along with significantly (p < 0.05) higher quantities of water soluble components (35 ± 0.95 g) and cellulose content (33 ± 0.18 g), as compared to the untreated substrate.
34678541	11	22	theme	raw	2013:2015	arg1	materials					2017:2025	inexpensive and renewable raw materials	1987:2025	inexpensive and renewable raw materials	1987:2025	Thus, the present study was successful in optimizing the biological pre-treatment of sugarcane bagasse and utilizing the resultant sugar-rich hydrolysates, as inexpensive and renewable raw materials, for PHA production.
34678541	0	23	theme	sugarcane	67:75	arg1	pre-treatment					50:62	biological pre-treatment	39:62	biological pre-treatment of sugarcane	39:75	Dual phase statistical optimization of biological pre-treatment of sugarcane bagasse with Pycnoporus coccineus MScMS1 for polyhydroxyalkanoates production.
34678541	5	24	theme	sugar	972:976	arg1	concentration					978:990	Optimized sugar concentration	962:990	Optimized sugar concentration	962:990	Optimized sugar concentration was approx.
34678541	8	25	theme	xylose	1409:1414	arg1	levels					1399:1404	Appreciable levels	1387:1404	Appreciable levels of xylose, arabinose, glucose and galactose	1387:1448	Appreciable levels of xylose, arabinose, glucose and galactose were detected in hydrolysates from biologically pre-treated bagasse.
34678541	7	26	theme	soluble	1277:1283	arg1	35 ± 0.95 g					1297:1307	35 ± 0.95 g	1297:1307	35 ± 0.95 g	1297:1307	Substrate compositional analysis revealed significant (p < 0.05) reduction of lignin by 70% in the biologically pre-treated substrate, along with significantly (p < 0.05) higher quantities of water soluble components (35 ± 0.95 g) and cellulose content (33 ± 0.18 g), as compared to the untreated substrate.
34678541	7	26	theme	soluble	1277:1283	arg1	components					1285:1294	water soluble components	1271:1294	water soluble components (35 ± 0.95 g)	1271:1308	Substrate compositional analysis revealed significant (p < 0.05) reduction of lignin by 70% in the biologically pre-treated substrate, along with significantly (p < 0.05) higher quantities of water soluble components (35 ± 0.95 g) and cellulose content (33 ± 0.18 g), as compared to the untreated substrate.
34678541	4	27	from	reduction	930:938	arg1	time					956:959	fermentation time	943:959	fermentation time	943:959	Amplification of the organism's functioning resulted in an enhancement of sugar productivity and yield accompanied by a significant reduction in fermentation time.
34678541	6	28	theme	4.5 g/	1064:1069	arg1	productivity					1048:1059	productivity	1048:1059	productivity of 4.5 g/(L.day)	1048:1076	18 g/L within 4 days of pre-treatment, with productivity of 4.5 g/(L.day).
34678541	8	29	theme	arabinose	1417:1425	arg1	levels					1399:1404	Appreciable levels	1387:1404	Appreciable levels of xylose, arabinose, glucose and galactose	1387:1448	Appreciable levels of xylose, arabinose, glucose and galactose were detected in hydrolysates from biologically pre-treated bagasse.
34678541	9	30	theme	sugar-rich	1623:1632	arg1	hydrolysates					1634:1645	these sugar-rich hydrolysates	1617:1645	these sugar-rich hydrolysates	1617:1645	Furthermore, Bacillus megaterium Ti3, a potent polyhydroxyalkanoates (PHA) producer, was grown on these sugar-rich hydrolysates and generated 0.58 g/L PHA in 24 h of fermentation accompanied by 0.88 g/L dry cell weight and 65% PHA accumulation.
34678541	2	31	theme	degrading	505:513	arg1	organisms					515:523	lignin degrading organisms	498:523	lignin degrading organisms	498:523	Despite numerous environmental benefits, biological pre-treatment has been side-lined due to its prolonged periods of fermentation, ascribed to the slow growth rate of lignin degrading organisms.
34678541	8	32	theme	glucose	1428:1434	arg1	levels					1399:1404	Appreciable levels	1387:1404	Appreciable levels of xylose, arabinose, glucose and galactose	1387:1448	Appreciable levels of xylose, arabinose, glucose and galactose were detected in hydrolysates from biologically pre-treated bagasse.
34678541	2	33	theme	fermentation	448:459	arg1	periods					437:443	its prolonged periods	423:443	its prolonged periods	423:443	Despite numerous environmental benefits, biological pre-treatment has been side-lined due to its prolonged periods of fermentation, ascribed to the slow growth rate of lignin degrading organisms.
34678541	0	34	theme	Dual	0:3	arg1	optimization					23:34	Dual phase statistical optimization	0:34	Dual phase statistical optimization of biological pre-treatment of sugarcane	0:75	Dual phase statistical optimization of biological pre-treatment of sugarcane bagasse with Pycnoporus coccineus MScMS1 for polyhydroxyalkanoates production.
34678541	8	35	theme	galactose	1440:1448	arg1	levels					1399:1404	Appreciable levels	1387:1404	Appreciable levels of xylose, arabinose, glucose and galactose	1387:1448	Appreciable levels of xylose, arabinose, glucose and galactose were detected in hydrolysates from biologically pre-treated bagasse.
34678541	2	36	theme	growth	483:488	arg1	rate					490:493	the slow growth rate	474:493	the slow growth rate of lignin degrading organisms	474:523	Despite numerous environmental benefits, biological pre-treatment has been side-lined due to its prolonged periods of fermentation, ascribed to the slow growth rate of lignin degrading organisms.
34678541	0	37	theme	statistical	11:21	arg1	optimization					23:34	Dual phase statistical optimization	0:34	Dual phase statistical optimization of biological pre-treatment of sugarcane	0:75	Dual phase statistical optimization of biological pre-treatment of sugarcane bagasse with Pycnoporus coccineus MScMS1 for polyhydroxyalkanoates production.
34678541	10	38	theme	glucose	1812:1818	arg1	medium					1820:1825	a glucose medium	1810:1825	a glucose medium	1810:1825	These results were comparable with those from a glucose medium.
34678541	9	39	theme	0.58 g/L	1661:1668	arg1	PHA					1670:1672	0.58 g/L PHA	1661:1672	0.58 g/L PHA	1661:1672	Furthermore, Bacillus megaterium Ti3, a potent polyhydroxyalkanoates (PHA) producer, was grown on these sugar-rich hydrolysates and generated 0.58 g/L PHA in 24 h of fermentation accompanied by 0.88 g/L dry cell weight and 65% PHA accumulation.
34678541	7	40	theme	untreated	1366:1374	arg1	substrate					1376:1384	the untreated substrate	1362:1384	the untreated substrate	1362:1384	Substrate compositional analysis revealed significant (p < 0.05) reduction of lignin by 70% in the biologically pre-treated substrate, along with significantly (p < 0.05) higher quantities of water soluble components (35 ± 0.95 g) and cellulose content (33 ± 0.18 g), as compared to the untreated substrate.
34678541	9	41	theme	dry	1722:1724	arg1	weight					1731:1736	0.88 g/L dry cell weight	1713:1736	0.88 g/L dry cell weight	1713:1736	Furthermore, Bacillus megaterium Ti3, a potent polyhydroxyalkanoates (PHA) producer, was grown on these sugar-rich hydrolysates and generated 0.58 g/L PHA in 24 h of fermentation accompanied by 0.88 g/L dry cell weight and 65% PHA accumulation.
34678541	3	42	theme	Orthogonal	707:716	arg1	Array					718:722	Taguchi Orthogonal Array	699:722	Taguchi Orthogonal Array	699:722	Thus, the present work adopted a dual phase statistical optimization approach for the biological pre-treatment of sugarcane bagasse, with Pycnoporus coccineus MScMS1, using Taguchi Orthogonal Array, in conjunction with Response Surface Methodology, to address this issue.
34678541	11	43	theme	bagasse	1923:1929	arg1	pre-treatment					1896:1908	the biological pre-treatment	1881:1908	the biological pre-treatment of sugarcane bagasse	1881:1929	Thus, the present study was successful in optimizing the biological pre-treatment of sugarcane bagasse and utilizing the resultant sugar-rich hydrolysates, as inexpensive and renewable raw materials, for PHA production.
34678541	0	44	theme	pre-treatment	50:62	arg1	optimization					23:34	Dual phase statistical optimization	0:34	Dual phase statistical optimization of biological pre-treatment of sugarcane	0:75	Dual phase statistical optimization of biological pre-treatment of sugarcane bagasse with Pycnoporus coccineus MScMS1 for polyhydroxyalkanoates production.
34678541	3	45	theme	Response	745:752	arg1	Methodology					762:772	Response Surface Methodology	745:772	Response Surface Methodology	745:772	Thus, the present work adopted a dual phase statistical optimization approach for the biological pre-treatment of sugarcane bagasse, with Pycnoporus coccineus MScMS1, using Taguchi Orthogonal Array, in conjunction with Response Surface Methodology, to address this issue.
34678541	9	46	dep	Bacillus	1532:1539	arg1	megaterium					1541:1550	megaterium	1541:1550	megaterium	1541:1550	Furthermore, Bacillus megaterium Ti3, a potent polyhydroxyalkanoates (PHA) producer, was grown on these sugar-rich hydrolysates and generated 0.58 g/L PHA in 24 h of fermentation accompanied by 0.88 g/L dry cell weight and 65% PHA accumulation.
34678541	1	47	theme	lignin	261:266	arg1	organisms					278:286	lignin degrading organisms	261:286	lignin degrading organisms	261:286	Biological pre-treatment is the removal of recalcitrant lignin from lignocellulose through the action of lignin degrading organisms and/or their ligninolytic enzymes system.
34678541	4	48	theme	sugar	872:876	arg1	productivity					878:889	sugar productivity	872:889	sugar productivity	872:889	Amplification of the organism's functioning resulted in an enhancement of sugar productivity and yield accompanied by a significant reduction in fermentation time.
34678541	3	49	theme	phase	564:568	arg1	approach					595:602	a dual phase statistical optimization approach	557:602	a dual phase statistical optimization approach	557:602	Thus, the present work adopted a dual phase statistical optimization approach for the biological pre-treatment of sugarcane bagasse, with Pycnoporus coccineus MScMS1, using Taguchi Orthogonal Array, in conjunction with Response Surface Methodology, to address this issue.
34678541	1	50	theme	organisms	278:286	arg1	system					322:327	lignin degrading organisms and/or their ligninolytic enzymes system	261:327	lignin degrading organisms and/or their ligninolytic enzymes system	261:327	Biological pre-treatment is the removal of recalcitrant lignin from lignocellulose through the action of lignin degrading organisms and/or their ligninolytic enzymes system.
34678541	9	51	theme	0.88 g/L	1713:1720	arg1	weight					1731:1736	0.88 g/L dry cell weight	1713:1736	0.88 g/L dry cell weight	1713:1736	Furthermore, Bacillus megaterium Ti3, a potent polyhydroxyalkanoates (PHA) producer, was grown on these sugar-rich hydrolysates and generated 0.58 g/L PHA in 24 h of fermentation accompanied by 0.88 g/L dry cell weight and 65% PHA accumulation.
34678541	7	52	theme	components	1285:1294	arg1	quantities					1257:1266	significantly (p < 0.05) higher quantities	1225:1266	significantly (p < 0.05) higher quantities of water soluble components (35 ± 0.95 g) and cellulose content (33 ± 0.18 g)	1225:1344	Substrate compositional analysis revealed significant (p < 0.05) reduction of lignin by 70% in the biologically pre-treated substrate, along with significantly (p < 0.05) higher quantities of water soluble components (35 ± 0.95 g) and cellulose content (33 ± 0.18 g), as compared to the untreated substrate.
34678541	7	53	theme	pre-treated	1191:1201	arg1	substrate					1203:1211	the biologically pre-treated substrate	1174:1211	the biologically pre-treated substrate	1174:1211	Substrate compositional analysis revealed significant (p < 0.05) reduction of lignin by 70% in the biologically pre-treated substrate, along with significantly (p < 0.05) higher quantities of water soluble components (35 ± 0.95 g) and cellulose content (33 ± 0.18 g), as compared to the untreated substrate.
34678541	9	54	theme	cell	1726:1729	arg1	weight					1731:1736	0.88 g/L dry cell weight	1713:1736	0.88 g/L dry cell weight	1713:1736	Furthermore, Bacillus megaterium Ti3, a potent polyhydroxyalkanoates (PHA) producer, was grown on these sugar-rich hydrolysates and generated 0.58 g/L PHA in 24 h of fermentation accompanied by 0.88 g/L dry cell weight and 65% PHA accumulation.
34678541	7	55	theme	cellulose	1314:1322	arg1	content					1324:1330	cellulose content	1314:1330	cellulose content (33 ± 0.18 g)	1314:1344	Substrate compositional analysis revealed significant (p < 0.05) reduction of lignin by 70% in the biologically pre-treated substrate, along with significantly (p < 0.05) higher quantities of water soluble components (35 ± 0.95 g) and cellulose content (33 ± 0.18 g), as compared to the untreated substrate.
34678541	7	55	theme	cellulose	1314:1322	arg1	33 ± 0.18 g					1333:1343	33 ± 0.18 g	1333:1343	33 ± 0.18 g	1333:1343	Substrate compositional analysis revealed significant (p < 0.05) reduction of lignin by 70% in the biologically pre-treated substrate, along with significantly (p < 0.05) higher quantities of water soluble components (35 ± 0.95 g) and cellulose content (33 ± 0.18 g), as compared to the untreated substrate.
34678541	2	56	theme	environmental	347:359	arg1	benefits					361:368	numerous environmental benefits	338:368	numerous environmental benefits	338:368	Despite numerous environmental benefits, biological pre-treatment has been side-lined due to its prolonged periods of fermentation, ascribed to the slow growth rate of lignin degrading organisms.
34678541	1	57	theme	enzymes	314:320	arg1	system					322:327	lignin degrading organisms and/or their ligninolytic enzymes system	261:327	lignin degrading organisms and/or their ligninolytic enzymes system	261:327	Biological pre-treatment is the removal of recalcitrant lignin from lignocellulose through the action of lignin degrading organisms and/or their ligninolytic enzymes system.
34678541	11	58	theme	sugarcane	1913:1921	arg1	bagasse					1923:1929	sugarcane bagasse	1913:1929	sugarcane bagasse	1913:1929	Thus, the present study was successful in optimizing the biological pre-treatment of sugarcane bagasse and utilizing the resultant sugar-rich hydrolysates, as inexpensive and renewable raw materials, for PHA production.
34678541	3	59	theme	optimization	582:593	arg1	approach					595:602	a dual phase statistical optimization approach	557:602	a dual phase statistical optimization approach	557:602	Thus, the present work adopted a dual phase statistical optimization approach for the biological pre-treatment of sugarcane bagasse, with Pycnoporus coccineus MScMS1, using Taguchi Orthogonal Array, in conjunction with Response Surface Methodology, to address this issue.
34678541	3	60	with	conjunction	728:738	arg1	Methodology					762:772	Response Surface Methodology	745:772	Response Surface Methodology	745:772	Thus, the present work adopted a dual phase statistical optimization approach for the biological pre-treatment of sugarcane bagasse, with Pycnoporus coccineus MScMS1, using Taguchi Orthogonal Array, in conjunction with Response Surface Methodology, to address this issue.
34678541	1	61	theme	recalcitrant	199:210	arg1	lignin					212:217	recalcitrant lignin	199:217	recalcitrant lignin	199:217	Biological pre-treatment is the removal of recalcitrant lignin from lignocellulose through the action of lignin degrading organisms and/or their ligninolytic enzymes system.
34678541	9	62	theme	%	1744:1744	arg1	accumulation					1750:1761	65% PHA accumulation	1742:1761	65% PHA accumulation	1742:1761	Furthermore, Bacillus megaterium Ti3, a potent polyhydroxyalkanoates (PHA) producer, was grown on these sugar-rich hydrolysates and generated 0.58 g/L PHA in 24 h of fermentation accompanied by 0.88 g/L dry cell weight and 65% PHA accumulation.
34678541	8	63	located	detected	1455:1462	arg2	levels					1399:1404	Appreciable levels	1387:1404	Appreciable levels of xylose, arabinose, glucose and galactose	1387:1448	Appreciable levels of xylose, arabinose, glucose and galactose were detected in hydrolysates from biologically pre-treated bagasse.
34678541	8	63	located	detected	1455:1462	arg1	hydrolysates					1467:1478	hydrolysates	1467:1478	hydrolysates from biologically pre-treated bagasse	1467:1516	Appreciable levels of xylose, arabinose, glucose and galactose were detected in hydrolysates from biologically pre-treated bagasse.
34678541	4	64	theme	significant	918:928	arg1	reduction					930:938	a significant reduction	916:938	a significant reduction in fermentation time	916:959	Amplification of the organism's functioning resulted in an enhancement of sugar productivity and yield accompanied by a significant reduction in fermentation time.
34678541	3	65	theme	biological	612:621	arg1	pre-treatment					623:635	the biological pre-treatment	608:635	the biological pre-treatment of sugarcane bagasse	608:656	Thus, the present work adopted a dual phase statistical optimization approach for the biological pre-treatment of sugarcane bagasse, with Pycnoporus coccineus MScMS1, using Taguchi Orthogonal Array, in conjunction with Response Surface Methodology, to address this issue.
34678541	3	66	theme	sugarcane	640:648	arg1	bagasse					650:656	sugarcane bagasse	640:656	sugarcane bagasse	640:656	Thus, the present work adopted a dual phase statistical optimization approach for the biological pre-treatment of sugarcane bagasse, with Pycnoporus coccineus MScMS1, using Taguchi Orthogonal Array, in conjunction with Response Surface Methodology, to address this issue.
34678541	11	67	theme	PHA	2032:2034	arg1	production					2036:2045	PHA production	2032:2045	PHA production	2032:2045	Thus, the present study was successful in optimizing the biological pre-treatment of sugarcane bagasse and utilizing the resultant sugar-rich hydrolysates, as inexpensive and renewable raw materials, for PHA production.
34678541	9	68	theme	Bacillus	1532:1539	arg1	Ti3					1552:1554	Bacillus megaterium Ti3	1532:1554	Bacillus megaterium Ti3	1532:1554	Furthermore, Bacillus megaterium Ti3, a potent polyhydroxyalkanoates (PHA) producer, was grown on these sugar-rich hydrolysates and generated 0.58 g/L PHA in 24 h of fermentation accompanied by 0.88 g/L dry cell weight and 65% PHA accumulation.
34678541	9	68	theme	Bacillus	1532:1539	arg1	producer					1594:1601	a potent polyhydroxyalkanoates (PHA) producer	1557:1601	a potent polyhydroxyalkanoates (PHA) producer	1557:1601	Furthermore, Bacillus megaterium Ti3, a potent polyhydroxyalkanoates (PHA) producer, was grown on these sugar-rich hydrolysates and generated 0.58 g/L PHA in 24 h of fermentation accompanied by 0.88 g/L dry cell weight and 65% PHA accumulation.
34678541	6	69	theme	pre-treatment	1028:1040	arg1	days					1020:1023	4 days	1018:1023	4 days of pre-treatment, with productivity of 4.5 g/(L.day)	1018:1076	18 g/L within 4 days of pre-treatment, with productivity of 4.5 g/(L.day).
34678541	3	70	theme	bagasse	650:656	arg1	pre-treatment					623:635	the biological pre-treatment	608:635	the biological pre-treatment of sugarcane bagasse	608:656	Thus, the present work adopted a dual phase statistical optimization approach for the biological pre-treatment of sugarcane bagasse, with Pycnoporus coccineus MScMS1, using Taguchi Orthogonal Array, in conjunction with Response Surface Methodology, to address this issue.
34678541	9	71	theme	potent	1559:1564	arg1	PHA					1589:1591	PHA	1589:1591	PHA	1589:1591	Furthermore, Bacillus megaterium Ti3, a potent polyhydroxyalkanoates (PHA) producer, was grown on these sugar-rich hydrolysates and generated 0.58 g/L PHA in 24 h of fermentation accompanied by 0.88 g/L dry cell weight and 65% PHA accumulation.
34678541	9	71	theme	potent	1559:1564	arg1	polyhydroxyalkanoates					1566:1586	potent polyhydroxyalkanoates	1559:1586	a potent polyhydroxyalkanoates (PHA) producer	1557:1601	Furthermore, Bacillus megaterium Ti3, a potent polyhydroxyalkanoates (PHA) producer, was grown on these sugar-rich hydrolysates and generated 0.58 g/L PHA in 24 h of fermentation accompanied by 0.88 g/L dry cell weight and 65% PHA accumulation.
34678541	7	72	theme	Substrate	1079:1087	arg1	analysis					1103:1110	Substrate compositional analysis	1079:1110	Substrate compositional analysis	1079:1110	Substrate compositional analysis revealed significant (p < 0.05) reduction of lignin by 70% in the biologically pre-treated substrate, along with significantly (p < 0.05) higher quantities of water soluble components (35 ± 0.95 g) and cellulose content (33 ± 0.18 g), as compared to the untreated substrate.
34678541	1	73	theme	Biological	156:165	arg1	pre-treatment					167:179	Biological pre-treatment	156:179	Biological pre-treatment	156:179	Biological pre-treatment is the removal of recalcitrant lignin from lignocellulose through the action of lignin degrading organisms and/or their ligninolytic enzymes system.
34678541	1	73	theme	Biological	156:165	arg1	removal					188:194	the removal	184:194	the removal of recalcitrant lignin from lignocellulose	184:237	Biological pre-treatment is the removal of recalcitrant lignin from lignocellulose through the action of lignin degrading organisms and/or their ligninolytic enzymes system.
34678541	1	74	from	lignocellulose	224:237	arg1	pre-treatment					167:179	Biological pre-treatment	156:179	Biological pre-treatment	156:179	Biological pre-treatment is the removal of recalcitrant lignin from lignocellulose through the action of lignin degrading organisms and/or their ligninolytic enzymes system.
34678541	1	74	from	lignocellulose	224:237	arg1	removal					188:194	the removal	184:194	the removal of recalcitrant lignin from lignocellulose	184:237	Biological pre-treatment is the removal of recalcitrant lignin from lignocellulose through the action of lignin degrading organisms and/or their ligninolytic enzymes system.
34678541	5	75	theme	Optimized	962:970	arg1	concentration					978:990	Optimized sugar concentration	962:990	Optimized sugar concentration	962:990	Optimized sugar concentration was approx.
34678541	0	76	theme	Pycnoporus	90:99	arg1	MScMS1					111:116	Pycnoporus coccineus MScMS1	90:116	Pycnoporus coccineus MScMS1 for polyhydroxyalkanoates production	90:153	Dual phase statistical optimization of biological pre-treatment of sugarcane bagasse with Pycnoporus coccineus MScMS1 for polyhydroxyalkanoates production.
34678541	8	77	theme	Appreciable	1387:1397	arg1	levels					1399:1404	Appreciable levels	1387:1404	Appreciable levels of xylose, arabinose, glucose and galactose	1387:1448	Appreciable levels of xylose, arabinose, glucose and galactose were detected in hydrolysates from biologically pre-treated bagasse.
34678541	11	78	theme	present	1838:1844	arg1	successful					1856:1865	successful	1856:1865	successful	1856:1865	Thus, the present study was successful in optimizing the biological pre-treatment of sugarcane bagasse and utilizing the resultant sugar-rich hydrolysates, as inexpensive and renewable raw materials, for PHA production.
34678541	11	78	theme	present	1838:1844	arg1	study					1846:1850	the present study	1834:1850	the present study	1834:1850	Thus, the present study was successful in optimizing the biological pre-treatment of sugarcane bagasse and utilizing the resultant sugar-rich hydrolysates, as inexpensive and renewable raw materials, for PHA production.
34678541	7	79	dep	higher	1250:1255	arg1	p < 0.05					1240:1247	p < 0.05	1240:1247	p < 0.05	1240:1247	Substrate compositional analysis revealed significant (p < 0.05) reduction of lignin by 70% in the biologically pre-treated substrate, along with significantly (p < 0.05) higher quantities of water soluble components (35 ± 0.95 g) and cellulose content (33 ± 0.18 g), as compared to the untreated substrate.
34678541	3	80	theme	present	536:542	arg1	work					544:547	the present work	532:547	the present work	532:547	Thus, the present work adopted a dual phase statistical optimization approach for the biological pre-treatment of sugarcane bagasse, with Pycnoporus coccineus MScMS1, using Taguchi Orthogonal Array, in conjunction with Response Surface Methodology, to address this issue.
34678541	0	81	theme	polyhydroxyalkanoates	122:142	arg1	production					144:153	polyhydroxyalkanoates production	122:153	polyhydroxyalkanoates production	122:153	Dual phase statistical optimization of biological pre-treatment of sugarcane bagasse with Pycnoporus coccineus MScMS1 for polyhydroxyalkanoates production.
34678541	11	82	theme	inexpensive	1987:1997	arg1	materials					2017:2025	inexpensive and renewable raw materials	1987:2025	inexpensive and renewable raw materials	1987:2025	Thus, the present study was successful in optimizing the biological pre-treatment of sugarcane bagasse and utilizing the resultant sugar-rich hydrolysates, as inexpensive and renewable raw materials, for PHA production.
34678541	0	83	theme	phase	5:9	arg1	optimization					23:34	Dual phase statistical optimization	0:34	Dual phase statistical optimization of biological pre-treatment of sugarcane	0:75	Dual phase statistical optimization of biological pre-treatment of sugarcane bagasse with Pycnoporus coccineus MScMS1 for polyhydroxyalkanoates production.
34678541	0	84	dep	Pycnoporus	90:99	arg1	coccineus					101:109	coccineus	101:109	coccineus	101:109	Dual phase statistical optimization of biological pre-treatment of sugarcane bagasse with Pycnoporus coccineus MScMS1 for polyhydroxyalkanoates production.
34678541	2	85	theme	lignin	498:503	arg1	organisms					515:523	lignin degrading organisms	498:523	lignin degrading organisms	498:523	Despite numerous environmental benefits, biological pre-treatment has been side-lined due to its prolonged periods of fermentation, ascribed to the slow growth rate of lignin degrading organisms.
34678541	7	86	from	%	1169:1169	arg1	substrate					1203:1211	the biologically pre-treated substrate	1174:1211	the biologically pre-treated substrate	1174:1211	Substrate compositional analysis revealed significant (p < 0.05) reduction of lignin by 70% in the biologically pre-treated substrate, along with significantly (p < 0.05) higher quantities of water soluble components (35 ± 0.95 g) and cellulose content (33 ± 0.18 g), as compared to the untreated substrate.
34678541	2	87	theme	slow	478:481	arg1	rate					490:493	the slow growth rate	474:493	the slow growth rate of lignin degrading organisms	474:523	Despite numerous environmental benefits, biological pre-treatment has been side-lined due to its prolonged periods of fermentation, ascribed to the slow growth rate of lignin degrading organisms.
34678541	0	88	theme	biological	39:48	arg1	pre-treatment					50:62	biological pre-treatment	39:62	biological pre-treatment of sugarcane	39:75	Dual phase statistical optimization of biological pre-treatment of sugarcane bagasse with Pycnoporus coccineus MScMS1 for polyhydroxyalkanoates production.
34678541	11	89	theme	resultant	1949:1957	arg1	hydrolysates					1970:1981	the resultant sugar-rich hydrolysates	1945:1981	the resultant sugar-rich hydrolysates	1945:1981	Thus, the present study was successful in optimizing the biological pre-treatment of sugarcane bagasse and utilizing the resultant sugar-rich hydrolysates, as inexpensive and renewable raw materials, for PHA production.
34678541	3	90	theme	Taguchi	699:705	arg1	Array					718:722	Taguchi Orthogonal Array	699:722	Taguchi Orthogonal Array	699:722	Thus, the present work adopted a dual phase statistical optimization approach for the biological pre-treatment of sugarcane bagasse, with Pycnoporus coccineus MScMS1, using Taguchi Orthogonal Array, in conjunction with Response Surface Methodology, to address this issue.
34678541	4	91	theme	productivity	878:889	arg1	enhancement					857:867	an enhancement	854:867	an enhancement of sugar productivity and yield accompanied by a significant reduction in fermentation time	854:959	Amplification of the organism's functioning resulted in an enhancement of sugar productivity and yield accompanied by a significant reduction in fermentation time.
34678541	11	92	theme	renewable	2003:2011	arg1	materials					2017:2025	inexpensive and renewable raw materials	1987:2025	inexpensive and renewable raw materials	1987:2025	Thus, the present study was successful in optimizing the biological pre-treatment of sugarcane bagasse and utilizing the resultant sugar-rich hydrolysates, as inexpensive and renewable raw materials, for PHA production.
34678541	2	93	theme	organisms	515:523	arg1	rate					490:493	the slow growth rate	474:493	the slow growth rate of lignin degrading organisms	474:523	Despite numerous environmental benefits, biological pre-treatment has been side-lined due to its prolonged periods of fermentation, ascribed to the slow growth rate of lignin degrading organisms.
34678541	4	94	theme	yield	895:899	arg1	enhancement					857:867	an enhancement	854:867	an enhancement of sugar productivity and yield accompanied by a significant reduction in fermentation time	854:959	Amplification of the organism's functioning resulted in an enhancement of sugar productivity and yield accompanied by a significant reduction in fermentation time.
34678541	10	95	with	comparable	1783:1792	arg1	those					1799:1803	those	1799:1803	those	1799:1803	These results were comparable with those from a glucose medium.
34678541	9	96	theme	fermentation	1685:1696	arg1	24 h					1677:1680	24 h	1677:1680	24 h of fermentation accompanied by 0.88 g/L dry cell weight and 65% PHA accumulation	1677:1761	Furthermore, Bacillus megaterium Ti3, a potent polyhydroxyalkanoates (PHA) producer, was grown on these sugar-rich hydrolysates and generated 0.58 g/L PHA in 24 h of fermentation accompanied by 0.88 g/L dry cell weight and 65% PHA accumulation.
34678541	2	97	theme	prolonged	427:435	arg1	periods					437:443	its prolonged periods	423:443	its prolonged periods	423:443	Despite numerous environmental benefits, biological pre-treatment has been side-lined due to its prolonged periods of fermentation, ascribed to the slow growth rate of lignin degrading organisms.
34678541	1	98	theme	degrading	268:276	arg1	organisms					278:286	lignin degrading organisms	261:286	lignin degrading organisms	261:286	Biological pre-treatment is the removal of recalcitrant lignin from lignocellulose through the action of lignin degrading organisms and/or their ligninolytic enzymes system.
34678541	7	99	theme	content	1324:1330	arg1	quantities					1257:1266	significantly (p < 0.05) higher quantities	1225:1266	significantly (p < 0.05) higher quantities of water soluble components (35 ± 0.95 g) and cellulose content (33 ± 0.18 g)	1225:1344	Substrate compositional analysis revealed significant (p < 0.05) reduction of lignin by 70% in the biologically pre-treated substrate, along with significantly (p < 0.05) higher quantities of water soluble components (35 ± 0.95 g) and cellulose content (33 ± 0.18 g), as compared to the untreated substrate.
34678541	8	100	theme	pre-treated	1498:1508	arg1	bagasse					1510:1516	biologically pre-treated bagasse	1485:1516	biologically pre-treated bagasse	1485:1516	Appreciable levels of xylose, arabinose, glucose and galactose were detected in hydrolysates from biologically pre-treated bagasse.
34678541	3	101	theme	statistical	570:580	arg1	approach					595:602	a dual phase statistical optimization approach	557:602	a dual phase statistical optimization approach	557:602	Thus, the present work adopted a dual phase statistical optimization approach for the biological pre-treatment of sugarcane bagasse, with Pycnoporus coccineus MScMS1, using Taguchi Orthogonal Array, in conjunction with Response Surface Methodology, to address this issue.
34971639	3	0	theme	strength	594:601	arg1	%					587:587	sulphate content (0.29 ± 0.09) %	556:587	sulphate content (0.29 ± 0.09) %	556:587	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	3	0	theme	strength	594:601	arg1	g/cm2					615:619	gel strength (2242 ± 21) g/cm2	590:619	gel strength (2242 ± 21) g/cm2	590:619	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	2	1	theme	proteins	369:376	arg1	separation					355:364	the separation	351:364	the separation of proteins	351:376	Cross-linked agarose beads are also used as solid-phase matrices in size exclusion chromatography for the separation of proteins.
34971639	2	2	used	used	285:288	arg2	beads					270:274	Cross-linked agarose beads	249:274	Cross-linked agarose beads	249:274	Cross-linked agarose beads are also used as solid-phase matrices in size exclusion chromatography for the separation of proteins.
34971639	2	2	used	used	285:288	arg2	matrices					305:312	solid-phase matrices	293:312	solid-phase matrices	293:312	Cross-linked agarose beads are also used as solid-phase matrices in size exclusion chromatography for the separation of proteins.
34971639	7	3	theme	major	1128:1132	arg1	components					1134:1143	the major components	1124:1143	the major components of the membrane	1124:1159	Due to the biodegradable nature of the major components of the membrane, it has the potential for industrial applications.
34971639	6	4	theme	hours	1082:1086	arg1	period					1068:1073	a period	1066:1073	a period of six hours	1066:1086	The composite membrane showed a stable oil/water emulsion and protein separation performance over a period of six hours.
34971639	2	5	link	Cross-linked	249:260	arg1	beads					270:274	Cross-linked agarose beads	249:274	Cross-linked agarose beads	249:274	Cross-linked agarose beads are also used as solid-phase matrices in size exclusion chromatography for the separation of proteins.
34971639	2	5	link	Cross-linked	249:260	arg1	matrices					305:312	solid-phase matrices	293:312	solid-phase matrices	293:312	Cross-linked agarose beads are also used as solid-phase matrices in size exclusion chromatography for the separation of proteins.
34971639	2	6	theme	solid-phase	293:303	arg1	beads					270:274	Cross-linked agarose beads	249:274	Cross-linked agarose beads	249:274	Cross-linked agarose beads are also used as solid-phase matrices in size exclusion chromatography for the separation of proteins.
34971639	2	6	theme	solid-phase	293:303	arg1	matrices					305:312	solid-phase matrices	293:312	solid-phase matrices	293:312	Cross-linked agarose beads are also used as solid-phase matrices in size exclusion chromatography for the separation of proteins.
34971639	7	7	contain	has	1165:1167	arg1	it					1162:1163	it	1162:1163	it	1162:1163	Due to the biodegradable nature of the major components of the membrane, it has the potential for industrial applications.
34971639	7	7	contain	has	1165:1167	arg2	potential					1173:1181	the potential	1169:1181	the potential for industrial applications	1169:1209	Due to the biodegradable nature of the major components of the membrane, it has the potential for industrial applications.
34971639	0	8	theme	oil/water	98:106	arg1	separation					84:93	the separation	80:93	the separation of oil/water emulsion and protein	80:127	Preparation of seaweed polysaccharide based hydrophobic composite membranes for the separation of oil/water emulsion and protein.
34971639	3	9	theme	biopolymer	513:522	arg1	derivative					487:496	herein hydrophobic derivative	468:496	herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2]	468:620	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	0	10	theme	protein	121:127	arg1	separation					84:93	the separation	80:93	the separation of oil/water emulsion and protein	80:127	Preparation of seaweed polysaccharide based hydrophobic composite membranes for the separation of oil/water emulsion and protein.
34971639	7	11	theme	industrial	1187:1196	arg1	applications					1198:1209	industrial applications	1187:1209	industrial applications	1187:1209	Due to the biodegradable nature of the major components of the membrane, it has the potential for industrial applications.
34971639	4	12	theme	membrane	800:807	arg1	performance					771:781	The oil and BSA rejection performance	745:781	The oil and BSA rejection performance of the composite membrane	745:807	The oil and BSA rejection performance of the composite membrane was greater than 98%.
34971639	4	12	theme	membrane	800:807	arg1	%					828:828	greater than 98%	813:828	greater than 98%	813:828	The oil and BSA rejection performance of the composite membrane was greater than 98%.
34971639	6	13	theme	stable	1000:1005	arg1	performance					1049:1059	a stable oil/water emulsion and protein separation performance	998:1059	a stable oil/water emulsion and protein separation performance	998:1059	The composite membrane showed a stable oil/water emulsion and protein separation performance over a period of six hours.
34971639	6	14	theme	separation	1038:1047	arg1	performance					1049:1059	a stable oil/water emulsion and protein separation performance	998:1059	a stable oil/water emulsion and protein separation performance	998:1059	The composite membrane showed a stable oil/water emulsion and protein separation performance over a period of six hours.
34971639	4	15	theme	BSA	757:759	arg1	performance					771:781	The oil and BSA rejection performance	745:781	The oil and BSA rejection performance of the composite membrane	745:807	The oil and BSA rejection performance of the composite membrane was greater than 98%.
34971639	4	15	theme	BSA	757:759	arg1	%					828:828	greater than 98%	813:828	greater than 98%	813:828	The oil and BSA rejection performance of the composite membrane was greater than 98%.
34971639	3	16	dep	 × 10	541:545	arg1	g/mol					549:553	5 g/mol	547:553	5 g/mol	547:553	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	0	17	dep	oil/water	98:106	arg1	emulsion					108:115	emulsion	108:115	emulsion	108:115	Preparation of seaweed polysaccharide based hydrophobic composite membranes for the separation of oil/water emulsion and protein.
34971639	6	18	theme	protein	1030:1036	arg1	separation					1038:1047	protein separation	1030:1047	protein separation	1030:1047	The composite membrane showed a stable oil/water emulsion and protein separation performance over a period of six hours.
34971639	5	19	theme	protein	948:954	arg1	separation					956:965	protein separation	948:965	protein separation	948:965	The rejection rate increased with the increase in polymer content in the respective membranes for both oil/water and protein separation.
34971639	2	20	theme	exclusion	322:330	arg1	chromatography					332:345	size exclusion chromatography	317:345	size exclusion chromatography for the separation of proteins	317:376	Cross-linked agarose beads are also used as solid-phase matrices in size exclusion chromatography for the separation of proteins.
34971639	6	21	theme	composite	972:980	arg1	membrane					982:989	The composite membrane	968:989	The composite membrane	968:989	The composite membrane showed a stable oil/water emulsion and protein separation performance over a period of six hours.
34971639	3	22	theme	composite	705:713	arg1	membrane					715:722	a composite membrane	703:722	a composite membrane on polyester fabric	703:742	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	3	23	from	membrane	715:722	arg1	fabric					737:742	polyester fabric	727:742	polyester fabric	727:742	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	2	24	theme	size	317:320	arg1	chromatography					332:345	size exclusion chromatography	317:345	size exclusion chromatography for the separation of proteins	317:376	Cross-linked agarose beads are also used as solid-phase matrices in size exclusion chromatography for the separation of proteins.
34971639	3	25	theme	oil/water	436:444	arg1	separation					422:431	the separation	418:431	the separation of oil/water emulsion and protein	418:465	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	0	26	theme	polysaccharide	23:36	arg1	Preparation					0:10	Preparation	0:10	Preparation of seaweed polysaccharide	0:36	Preparation of seaweed polysaccharide based hydrophobic composite membranes for the separation of oil/water emulsion and protein.
34971639	1	27	used	used	186:189	arg2	polysaccharide					157:170	a seaweed-based polysaccharide	141:170	a seaweed-based polysaccharide	141:170	Agarose is a seaweed-based polysaccharide and is widely used for the separation of nucleic acids in molecular biology.
34971639	1	27	used	used	186:189	arg2	Agarose					130:136	Agarose	130:136	Agarose	130:136	Agarose is a seaweed-based polysaccharide and is widely used for the separation of nucleic acids in molecular biology.
34971639	3	28	theme	content	565:571	arg1	%					587:587	sulphate content (0.29 ± 0.09) %	556:587	sulphate content (0.29 ± 0.09) %	556:587	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	3	28	theme	content	565:571	arg1	g/cm2					615:619	gel strength (2242 ± 21) g/cm2	590:619	gel strength (2242 ± 21) g/cm2	590:619	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	5	29	theme	respective	904:913	arg1	membranes					915:923	the respective membranes	900:923	the respective membranes for both oil/water and protein separation	900:965	The rejection rate increased with the increase in polymer content in the respective membranes for both oil/water and protein separation.
34971639	0	30	theme	seaweed	15:21	arg1	polysaccharide					23:36	seaweed polysaccharide	15:36	seaweed polysaccharide	15:36	Preparation of seaweed polysaccharide based hydrophobic composite membranes for the separation of oil/water emulsion and protein.
34971639	3	31	theme	sulphate	556:563	arg1	%					587:587	sulphate content (0.29 ± 0.09) %	556:587	sulphate content (0.29 ± 0.09) %	556:587	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	3	31	theme	sulphate	556:563	arg1	g/cm2					615:619	gel strength (2242 ± 21) g/cm2	590:619	gel strength (2242 ± 21) g/cm2	590:619	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	3	32	theme	hydrophobic	475:485	arg1	derivative					487:496	herein hydrophobic derivative	468:496	herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2]	468:620	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	0	33	theme	hydrophobic	44:54	arg1	membranes					66:74	hydrophobic composite membranes	44:74	hydrophobic composite membranes	44:74	Preparation of seaweed polysaccharide based hydrophobic composite membranes for the separation of oil/water emulsion and protein.
34971639	3	34	dep	biopolymer	513:522	arg1	 × 10					541:545	 × 10	541:545	 × 10	541:545	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	7	35	theme	components	1134:1143	arg1	nature					1114:1119	the biodegradable nature	1096:1119	the biodegradable nature of the major components of the membrane	1096:1159	Due to the biodegradable nature of the major components of the membrane, it has the potential for industrial applications.
34971639	3	36	theme	protein	459:465	arg1	separation					422:431	the separation	418:431	the separation of oil/water emulsion and protein	418:465	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	6	37	theme	emulsion	1017:1024	arg1	performance					1049:1059	a stable oil/water emulsion and protein separation performance	998:1059	a stable oil/water emulsion and protein separation performance	998:1059	The composite membrane showed a stable oil/water emulsion and protein separation performance over a period of six hours.
34971639	1	38	theme	nucleic	213:219	arg1	acids					221:225	nucleic acids	213:225	nucleic acids	213:225	Agarose is a seaweed-based polysaccharide and is widely used for the separation of nucleic acids in molecular biology.
34971639	7	39	theme	biodegradable	1100:1112	arg1	nature					1114:1119	the biodegradable nature	1096:1119	the biodegradable nature of the major components of the membrane	1096:1159	Due to the biodegradable nature of the major components of the membrane, it has the potential for industrial applications.
34971639	3	40	dep	oil/water	436:444	arg1	emulsion					446:453	emulsion	446:453	emulsion	446:453	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	1	41	theme	acids	221:225	arg1	separation					199:208	the separation	195:208	the separation of nucleic acids in molecular biology	195:246	Agarose is a seaweed-based polysaccharide and is widely used for the separation of nucleic acids in molecular biology.
34971639	3	42	theme	agarose	406:412	arg1	application					391:401	the application	387:401	the application of agarose for the separation of oil/water emulsion and protein	387:465	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	0	43	theme	composite	56:64	arg1	membranes					66:74	hydrophobic composite membranes	44:74	hydrophobic composite membranes	44:74	Preparation of seaweed polysaccharide based hydrophobic composite membranes for the separation of oil/water emulsion and protein.
34971639	4	44	theme	composite	790:798	arg1	membrane					800:807	the composite membrane	786:807	the composite membrane	786:807	The oil and BSA rejection performance of the composite membrane was greater than 98%.
34971639	1	45	from	separation	199:208	arg1	biology					240:246	molecular biology	230:246	molecular biology	230:246	Agarose is a seaweed-based polysaccharide and is widely used for the separation of nucleic acids in molecular biology.
34971639	3	46	theme	seaweed	505:511	arg1	biopolymer					513:522	the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2]	501:620	the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2]	501:620	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	3	47	theme	polyester	727:735	arg1	fabric					737:742	polyester fabric	727:742	polyester fabric	727:742	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	5	48	theme	polymer	881:887	arg1	content					889:895	polymer content	881:895	polymer content in the respective membranes for both oil/water and protein separation	881:965	The rejection rate increased with the increase in polymer content in the respective membranes for both oil/water and protein separation.
34971639	5	49	from	increase	869:876	arg1	content					889:895	polymer content	881:895	polymer content in the respective membranes for both oil/water and protein separation	881:965	The rejection rate increased with the increase in polymer content in the respective membranes for both oil/water and protein separation.
34971639	4	50	theme	oil	749:751	arg1	performance					771:781	The oil and BSA rejection performance	745:781	The oil and BSA rejection performance of the composite membrane	745:807	The oil and BSA rejection performance of the composite membrane was greater than 98%.
34971639	4	50	theme	oil	749:751	arg1	%					828:828	greater than 98%	813:828	greater than 98%	813:828	The oil and BSA rejection performance of the composite membrane was greater than 98%.
34971639	4	51	theme	rejection	761:769	arg1	performance					771:781	The oil and BSA rejection performance	745:781	The oil and BSA rejection performance of the composite membrane	745:807	The oil and BSA rejection performance of the composite membrane was greater than 98%.
34971639	4	51	theme	rejection	761:769	arg1	%					828:828	greater than 98%	813:828	greater than 98%	813:828	The oil and BSA rejection performance of the composite membrane was greater than 98%.
34971639	3	52	theme	gel	590:592	arg1	%					587:587	sulphate content (0.29 ± 0.09) %	556:587	sulphate content (0.29 ± 0.09) %	556:587	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	3	52	theme	gel	590:592	arg1	g/cm2					615:619	gel strength (2242 ± 21) g/cm2	590:619	gel strength (2242 ± 21) g/cm2	590:619	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	3	53	theme	2242 ± 21	604:612	arg1	%					587:587	sulphate content (0.29 ± 0.09) %	556:587	sulphate content (0.29 ± 0.09) %	556:587	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	3	53	theme	2242 ± 21	604:612	arg1	g/cm2					615:619	gel strength (2242 ± 21) g/cm2	590:619	gel strength (2242 ± 21) g/cm2	590:619	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	3	54	dep	g/mol	549:553	arg1	%					587:587	sulphate content (0.29 ± 0.09) %	556:587	sulphate content (0.29 ± 0.09) %	556:587	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	3	54	dep	g/mol	549:553	arg1	g/cm2					615:619	gel strength (2242 ± 21) g/cm2	590:619	gel strength (2242 ± 21) g/cm2	590:619	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	1	55	theme	molecular	230:238	arg1	biology					240:246	molecular biology	230:246	molecular biology	230:246	Agarose is a seaweed-based polysaccharide and is widely used for the separation of nucleic acids in molecular biology.
34971639	2	56	theme	agarose	262:268	arg1	beads					270:274	Cross-linked agarose beads	249:274	Cross-linked agarose beads	249:274	Cross-linked agarose beads are also used as solid-phase matrices in size exclusion chromatography for the separation of proteins.
34971639	2	56	theme	agarose	262:268	arg1	matrices					305:312	solid-phase matrices	293:312	solid-phase matrices	293:312	Cross-linked agarose beads are also used as solid-phase matrices in size exclusion chromatography for the separation of proteins.
34971639	6	57	theme	oil/water	1007:1015	arg1	emulsion					1017:1024	oil/water emulsion	1007:1024	oil/water emulsion	1007:1024	The composite membrane showed a stable oil/water emulsion and protein separation performance over a period of six hours.
34971639	1	58	theme	seaweed-based	143:155	arg1	polysaccharide					157:170	a seaweed-based polysaccharide	141:170	a seaweed-based polysaccharide	141:170	Agarose is a seaweed-based polysaccharide and is widely used for the separation of nucleic acids in molecular biology.
34971639	1	58	theme	seaweed-based	143:155	arg1	Agarose					130:136	Agarose	130:136	Agarose	130:136	Agarose is a seaweed-based polysaccharide and is widely used for the separation of nucleic acids in molecular biology.
34971639	5	59	from	content	889:895	arg1	membranes					915:923	the respective membranes	900:923	the respective membranes for both oil/water and protein separation	900:965	The rejection rate increased with the increase in polymer content in the respective membranes for both oil/water and protein separation.
34971639	3	60	theme	stearic	666:672	arg1	acid					674:677	stearic acid	666:677	stearic acid	666:677	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	2	61	theme	Cross-linked	249:260	arg1	beads					270:274	Cross-linked agarose beads	249:274	Cross-linked agarose beads	249:274	Cross-linked agarose beads are also used as solid-phase matrices in size exclusion chromatography for the separation of proteins.
34971639	2	61	theme	Cross-linked	249:260	arg1	matrices					305:312	solid-phase matrices	293:312	solid-phase matrices	293:312	Cross-linked agarose beads are also used as solid-phase matrices in size exclusion chromatography for the separation of proteins.
34971639	5	62	theme	rejection	835:843	arg1	rate					845:848	The rejection rate	831:848	The rejection rate	831:848	The rejection rate increased with the increase in polymer content in the respective membranes for both oil/water and protein separation.
34971639	3	63	with	biopolymer	650:659	arg1	acid					674:677	stearic acid	666:677	stearic acid	666:677	To find the application of agarose for the separation of oil/water emulsion and protein, herein hydrophobic derivative of the seaweed biopolymer [MW (1.27 ± 0.17) × 10 5 g/mol; sulphate content (0.29 ± 0.09) %, gel strength (2242 ± 21) g/cm2] is prepared by reacting the biopolymer with stearic acid and was used to prepare a composite membrane on polyester fabric.
34971639	7	64	theme	membrane	1152:1159	arg1	components					1134:1143	the major components	1124:1143	the major components of the membrane	1124:1159	Due to the biodegradable nature of the major components of the membrane, it has the potential for industrial applications.
34144068	0	0	theme	annealed	87:94	arg1	starches					101:108	annealed rice starches	87:108	annealed rice starches	87:108	Removal of starch granule associated proteins alters the physicochemical properties of annealed rice starches.
34144068	2	1	theme	%	556:556	arg1	starch					562:567	Yangfunuo (1.56% AC) starch	541:567	Yangfunuo (1.56% AC) starch	541:567	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	1	theme	%	556:556	arg1	AC					558:559	1.56% AC	552:559	1.56% AC	552:559	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	2	theme	storage	623:629	arg1	modulus					631:637	storage modulus	623:637	storage modulus of all three rice starches	623:664	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	1	3	theme	associated	151:160	arg1	dual-treatment					194:207	dual-treatment	194:207	dual-treatment	194:207	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	1	3	theme	associated	151:160	arg1	SGAPs					172:176	SGAPs	172:176	SGAPs	172:176	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	1	3	theme	associated	151:160	arg1	annealing					180:188	annealing	180:188	annealing	180:188	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	1	3	theme	associated	151:160	arg1	proteins					162:169	starch granule associated proteins	136:169	starch granule associated proteins (SGAPs)	136:177	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	3	4	theme	starch	798:803	arg1	starches					717:724	the three starches	707:724	the three starches	707:724	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	3	4	theme	starch	798:803	arg1	modulus					777:783	storage modulus	769:783	storage modulus of Yangfunuo starch	769:803	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	1	5	theme	rice	248:251	arg1	starches					253:260	three rice starches	242:260	three rice starches with different amylose content (AC)	242:296	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	2	6	theme	%	468:468	arg1	Qiuguang					454:461	Qiuguang	454:461	Qiuguang (15.6% AC)	454:472	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	6	theme	%	468:468	arg1	AC					470:471	15.6% AC	464:471	15.6% AC	464:471	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	7	theme	1.56	552:555	arg1	%					556:556	%	556:556	%	556:556	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	3	8	theme	pasting	892:898	arg1	properties					900:909	pasting properties	892:909	pasting properties	892:909	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	1	9	theme	proteins	162:169	arg1	removal					125:131	removal	125:131	removal of starch granule associated proteins (SGAPs), annealing and dual-treatment	125:207	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	1	10	theme	starches	253:260	arg1	properties					228:237	physicochemical properties	212:237	physicochemical properties of three rice starches with different amylose content (AC)	212:296	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	0	11	theme	starches	101:108	arg1	properties					73:82	the physicochemical properties	53:82	the physicochemical properties of annealed rice starches	53:108	Removal of starch granule associated proteins alters the physicochemical properties of annealed rice starches.
34144068	4	12	theme	removal	1034:1040	arg1	effect					1018:1023	effect	1018:1023	effect of SGAPs removal and annealing treatment	1018:1064	The effect of dual-treatment was generally the sum of effect of SGAPs removal and annealing treatment.
34144068	6	13	theme	annealing	1163:1171	arg1	related					1185:1191	related	1185:1191	related	1185:1191	The effect of annealing was closely related to the variety and composition of the starch.
34144068	6	13	theme	annealing	1163:1171	arg1	effect					1153:1158	The effect	1149:1158	The effect of annealing	1149:1171	The effect of annealing was closely related to the variety and composition of the starch.
34144068	3	14	theme	properties	900:909	arg1	pseudoplasticity					915:930	pseudoplasticity	915:930	pseudoplasticity of Qiuguang and Luhui starches	915:961	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	3	14	theme	properties	900:909	arg1	power					829:833	swelling power	820:833	swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties	820:909	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	3	15	dep	Qiuguang	935:942	arg1	starches					954:961	starches	954:961	starches	954:961	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	0	16	theme	rice	96:99	arg1	starches					101:108	annealed rice starches	87:108	annealed rice starches	87:108	Removal of starch granule associated proteins alters the physicochemical properties of annealed rice starches.
34144068	2	17	theme	Yangfunuo	541:549	arg1	starch					562:567	Yangfunuo (1.56% AC) starch	541:567	Yangfunuo (1.56% AC) starch	541:567	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	17	theme	Yangfunuo	541:549	arg1	AC					558:559	1.56% AC	552:559	1.56% AC	552:559	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	18	theme	starch	351:356	arg1	granules					358:365	starch granules	351:365	starch granules	351:365	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	4	19	theme	effect	1018:1023	arg1	effect					968:973	The effect	964:973	The effect of dual-treatment	964:991	The effect of dual-treatment was generally the sum of effect of SGAPs removal and annealing treatment.
34144068	4	19	theme	effect	1018:1023	arg1	sum					1011:1013	the sum	1007:1013	the sum of effect of SGAPs removal and annealing treatment	1007:1064	The effect of dual-treatment was generally the sum of effect of SGAPs removal and annealing treatment.
34144068	1	20	theme	different	267:275	arg1	AC					294:295	AC	294:295	AC	294:295	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	1	20	theme	different	267:275	arg1	content					285:291	different amylose content	267:291	different amylose content (AC)	267:296	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	3	21	theme	amylose	687:693	arg1	leaching					695:702	amylose leaching	687:702	amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch,	687:804	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	5	22	theme	dual-treatment	1100:1113	arg1	effect					1086:1091	an interaction effect	1071:1091	an interaction effect of the dual-treatment	1071:1113	But an interaction effect of the dual-treatment was observed for some parameters.
34144068	2	23	theme	starches	657:664	arg1	viscosity					609:617	viscosity	609:617	viscosity	609:617	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	23	theme	starches	657:664	arg1	modulus					631:637	storage modulus	623:637	storage modulus of all three rice starches	623:664	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	24	theme	Luhui	478:482	arg1	pseudoplasticity					434:449	pseudoplasticity	434:449	pseudoplasticity	434:449	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	24	theme	Luhui	478:482	arg1	solubility					418:427	solubility	418:427	solubility	418:427	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	24	theme	Luhui	478:482	arg1	leaching					392:399	amylose leaching	384:399	amylose leaching	384:399	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	24	theme	Luhui	478:482	arg1	power					411:415	swelling power	402:415	swelling power	402:415	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	3	25	theme	pasting	731:737	arg1	properties					739:748	pasting properties	731:748	pasting properties	731:748	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	2	26	theme	15.6	464:467	arg1	%					468:468	%	468:468	%	468:468	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	27	dep	granules	358:365	arg1	decreasing					510:519	decreasing	510:519	decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch	510:567	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	27	dep	granules	358:365	arg1	decreasing					574:583	decreasing	574:583	decreasing To	574:586	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	27	dep	granules	358:365	arg1	increasing					373:382	increasing	373:382	increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches	373:507	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	28	theme	rice	652:655	arg1	starches					657:664	all three rice starches	642:664	all three rice starches	642:664	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	29	theme	amylose	384:390	arg1	leaching					392:399	amylose leaching	384:399	amylose leaching	384:399	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	5	30	theme	interaction	1074:1084	arg1	effect					1086:1091	an interaction effect	1071:1091	an interaction effect of the dual-treatment	1071:1113	But an interaction effect of the dual-treatment was observed for some parameters.
34144068	3	31	theme	Qiuguang	935:942	arg1	pseudoplasticity					915:930	pseudoplasticity	915:930	pseudoplasticity of Qiuguang and Luhui starches	915:961	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	3	31	theme	Qiuguang	935:942	arg1	power					829:833	swelling power	820:833	swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties	820:909	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	1	32	theme	amylose	277:283	arg1	AC					294:295	AC	294:295	AC	294:295	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	1	32	theme	amylose	277:283	arg1	content					285:291	different amylose content	267:291	different amylose content (AC)	267:296	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	1	33	from	effect	115:120	arg1	properties					228:237	physicochemical properties	212:237	physicochemical properties of three rice starches with different amylose content (AC)	212:296	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	0	34	theme	granule	18:24	arg1	proteins					37:44	starch granule associated proteins	11:44	starch granule associated proteins	11:44	Removal of starch granule associated proteins alters the physicochemical properties of annealed rice starches.
34144068	0	35	theme	starch	11:16	arg1	proteins					37:44	starch granule associated proteins	11:44	starch granule associated proteins	11:44	Removal of starch granule associated proteins alters the physicochemical properties of annealed rice starches.
34144068	2	36	theme	rice	495:498	arg1	starches					500:507	rice starches	495:507	rice starches	495:507	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	4	37	theme	annealing	1046:1054	arg1	treatment					1056:1064	annealing treatment	1046:1064	annealing treatment	1046:1064	The effect of dual-treatment was generally the sum of effect of SGAPs removal and annealing treatment.
34144068	0	38	theme	proteins	37:44	arg1	Removal					0:6	Removal	0:6	Removal of starch granule associated proteins	0:44	Removal of starch granule associated proteins alters the physicochemical properties of annealed rice starches.
34144068	3	39	theme	swelling	820:827	arg1	power					829:833	swelling power	820:833	swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties	820:909	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	2	40	theme	swelling	402:409	arg1	power					411:415	swelling power	402:415	swelling power	402:415	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	0	41	theme	associated	26:35	arg1	proteins					37:44	starch granule associated proteins	11:44	starch granule associated proteins	11:44	Removal of starch granule associated proteins alters the physicochemical properties of annealed rice starches.
34144068	2	42	dep	Qiuguang	454:461	arg1	starches					500:507	rice starches	495:507	rice starches	495:507	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	3	43	theme	properties	739:748	arg1	leaching					695:702	amylose leaching	687:702	amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch,	687:804	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	6	44	dep	variety	1200:1206	arg1	the					1196:1198	the	1196:1198	the	1196:1198	The effect of annealing was closely related to the variety and composition of the starch.
34144068	3	45	theme	ΔH	858:859	arg1	pseudoplasticity					915:930	pseudoplasticity	915:930	pseudoplasticity of Qiuguang and Luhui starches	915:961	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	3	45	theme	ΔH	858:859	arg1	power					829:833	swelling power	820:833	swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties	820:909	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	6	46	theme	starch	1231:1236	arg1	composition					1212:1222	composition	1212:1222	composition	1212:1222	The effect of annealing was closely related to the variety and composition of the starch.
34144068	6	46	theme	starch	1231:1236	arg1	starch					1231:1236	the starch	1227:1236	the starch	1227:1236	The effect of annealing was closely related to the variety and composition of the starch.
34144068	6	46	theme	starch	1231:1236	arg1	variety					1200:1206	variety	1200:1206	variety	1200:1206	The effect of annealing was closely related to the variety and composition of the starch.
34144068	2	47	theme	Qiuguang	454:461	arg1	pseudoplasticity					434:449	pseudoplasticity	434:449	pseudoplasticity	434:449	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	47	theme	Qiuguang	454:461	arg1	solubility					418:427	solubility	418:427	solubility	418:427	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	47	theme	Qiuguang	454:461	arg1	leaching					392:399	amylose leaching	384:399	amylose leaching	384:399	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	47	theme	Qiuguang	454:461	arg1	power					411:415	swelling power	402:415	swelling power	402:415	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	48	theme	%	489:489	arg1	AC					491:492	22.1% AC	485:492	22.1% AC	485:492	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	48	theme	%	489:489	arg1	Luhui					478:482	Luhui	478:482	Luhui (22.1% AC)	478:493	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	49	theme	granules	358:365	arg1	stability					338:346	stability	338:346	stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To	338:586	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	49	theme	granules	358:365	arg1	Tp					589:590	Tp	589:590	Tp	589:590	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	2	49	theme	granules	358:365	arg1	Tc					597:598	Tc	597:598	Tc	597:598	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	4	50	theme	SGAPs	1028:1032	arg1	removal					1034:1040	SGAPs removal	1028:1040	SGAPs removal	1028:1040	The effect of dual-treatment was generally the sum of effect of SGAPs removal and annealing treatment.
34144068	2	51	theme	starch	562:567	arg1	pseudoplasticity					521:536	pseudoplasticity	521:536	pseudoplasticity of Yangfunuo (1.56% AC) starch	521:567	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	3	52	theme	starches	848:855	arg1	pseudoplasticity					915:930	pseudoplasticity	915:930	pseudoplasticity of Qiuguang and Luhui starches	915:961	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	3	52	theme	starches	848:855	arg1	power					829:833	swelling power	820:833	swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties	820:909	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	1	53	with	starches	253:260	arg1	AC					294:295	AC	294:295	AC	294:295	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	1	53	with	starches	253:260	arg1	content					285:291	different amylose content	267:291	different amylose content (AC)	267:296	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	1	54	theme	removal	125:131	arg1	effect					115:120	The effect	111:120	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC)	111:296	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	3	55	theme	storage	769:775	arg1	starches					717:724	the three starches	707:724	the three starches	707:724	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	3	55	theme	storage	769:775	arg1	modulus					777:783	storage modulus	769:783	storage modulus of Yangfunuo starch	769:803	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	1	56	theme	physicochemical	212:226	arg1	properties					228:237	physicochemical properties	212:237	physicochemical properties of three rice starches with different amylose content (AC)	212:296	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	3	57	theme	Luhui	948:952	arg1	pseudoplasticity					915:930	pseudoplasticity	915:930	pseudoplasticity of Qiuguang and Luhui starches	915:961	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	3	57	theme	Luhui	948:952	arg1	power					829:833	swelling power	820:833	swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties	820:909	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	3	58	theme	Qiuguang	871:878	arg1	starch					880:885	Qiuguang starch	871:885	Qiuguang starch	871:885	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	2	59	theme	SGAPs	316:320	arg1	removal					322:328	SGAPs removal	316:328	SGAPs removal	316:328	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
34144068	4	60	theme	dual-treatment	978:991	arg1	effect					968:973	The effect	964:973	The effect of dual-treatment	964:991	The effect of dual-treatment was generally the sum of effect of SGAPs removal and annealing treatment.
34144068	4	60	theme	dual-treatment	978:991	arg1	sum					1011:1013	the sum	1007:1013	the sum of effect of SGAPs removal and annealing treatment	1007:1064	The effect of dual-treatment was generally the sum of effect of SGAPs removal and annealing treatment.
34144068	3	61	theme	starches	717:724	arg1	leaching					695:702	amylose leaching	687:702	amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch,	687:804	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	3	62	theme	starch	880:885	arg1	ΔH					858:859	ΔH	858:859	ΔH and To of Qiuguang starch	858:885	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	3	62	theme	starch	880:885	arg1	starches					848:855	the three starches	838:855	the three starches	838:855	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	3	62	theme	starch	880:885	arg1	properties					900:909	pasting properties	892:909	pasting properties	892:909	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	1	63	theme	starch	136:141	arg1	dual-treatment					194:207	dual-treatment	194:207	dual-treatment	194:207	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	1	63	theme	starch	136:141	arg1	SGAPs					172:176	SGAPs	172:176	SGAPs	172:176	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	1	63	theme	starch	136:141	arg1	annealing					180:188	annealing	180:188	annealing	180:188	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	1	63	theme	starch	136:141	arg1	proteins					162:169	starch granule associated proteins	136:169	starch granule associated proteins (SGAPs)	136:177	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	0	64	theme	physicochemical	57:71	arg1	properties					73:82	the physicochemical properties	53:82	the physicochemical properties of annealed rice starches	53:108	Removal of starch granule associated proteins alters the physicochemical properties of annealed rice starches.
34144068	4	65	theme	treatment	1056:1064	arg1	effect					1018:1023	effect	1018:1023	effect of SGAPs removal and annealing treatment	1018:1064	The effect of dual-treatment was generally the sum of effect of SGAPs removal and annealing treatment.
34144068	1	66	theme	granule	143:149	arg1	dual-treatment					194:207	dual-treatment	194:207	dual-treatment	194:207	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	1	66	theme	granule	143:149	arg1	SGAPs					172:176	SGAPs	172:176	SGAPs	172:176	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	1	66	theme	granule	143:149	arg1	annealing					180:188	annealing	180:188	annealing	180:188	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	1	66	theme	granule	143:149	arg1	proteins					162:169	starch granule associated proteins	136:169	starch granule associated proteins (SGAPs)	136:177	The effect of removal of starch granule associated proteins (SGAPs), annealing and dual-treatment on physicochemical properties of three rice starches with different amylose content (AC) was investigated.
34144068	3	67	theme	Yangfunuo	788:796	arg1	starch					798:803	Yangfunuo starch	788:803	Yangfunuo starch	788:803	Annealing decreased amylose leaching of the three starches, and pasting properties, pseudoplastic and storage modulus of Yangfunuo starch, but increased swelling power of the three starches, ΔH and To of Qiuguang starch, and pasting properties and pseudoplasticity of Qiuguang and Luhui starches.
34144068	2	68	theme	22.1	485:488	arg1	%					489:489	%	489:489	%	489:489	SGAPs removal reduced stability of starch granules, thus increasing amylose leaching, swelling power, solubility, and pseudoplasticity of Qiuguang (15.6% AC) and Luhui (22.1% AC) rice starches, decreasing pseudoplasticity of Yangfunuo (1.56% AC) starch, and decreasing To, Tp, and Tc, pasting viscosity and storage modulus of all three rice starches.
32518941	11	0	theme	minimal	1765:1771	arg1	length					1773:1778	a minimal length	1763:1778	a minimal length of peptide	1763:1789	Because the majority of antibody paratopes bind to the peptide portion with or without sugar modification, we propose a snake-catching model for predicted paratopes: a minimal length of peptide is first clamped by a paratope and sugar modifications close to the peptide either strengthen or do not hinder the binding.
32518941	2	1	theme	sugar	368:372	arg1	coat					374:377	the sugar coat	364:377	the sugar coat	364:377	The mechanisms by which human antibodies may recognize the antigenic viral peptide epitopes hidden by the sugar coat are unknown.
32518941	10	2	theme	central	1525:1531	arg1	helix					1533:1537	central helix	1525:1537	central helix	1525:1537	Other surface-exposed domains include those located on central helix, connecting region, heptad repeats and N-terminal domain.
32518941	4	3	theme	SARS-CoV-2	666:675	arg1	protein					684:690	recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein	605:690	recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells	605:730	In this study, we analyzed recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells, by trypsin and chymotrypsin digestion followed by mass spectrometry analysis.
32518941	9	4	theme	glycans	1287:1293	arg1	densities					1274:1282	Electron densities	1265:1282	Electron densities of glycans	1265:1293	Electron densities of glycans cover most of the spike receptor-binding domain of SARS-CoV-2, except YQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQ, similar to a region FSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQ in SARS-CoV-1.
32518941	2	5	theme	human	286:290	arg1	antibodies					292:301	human antibodies	286:301	human antibodies	286:301	The mechanisms by which human antibodies may recognize the antigenic viral peptide epitopes hidden by the sugar coat are unknown.
32518941	4	6	theme	spike	678:682	arg1	protein					684:690	recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein	605:690	recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells	605:730	In this study, we analyzed recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells, by trypsin and chymotrypsin digestion followed by mass spectrometry analysis.
32518941	2	7	theme	viral	331:335	arg1	epitopes					345:352	the antigenic viral peptide epitopes	317:352	the antigenic viral peptide epitopes hidden by the sugar coat	317:377	The mechanisms by which human antibodies may recognize the antigenic viral peptide epitopes hidden by the sugar coat are unknown.
32518941	4	8	theme	syndrome	642:649	arg1	protein					684:690	recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein	605:690	recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells	605:730	In this study, we analyzed recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells, by trypsin and chymotrypsin digestion followed by mass spectrometry analysis.
32518941	7	9	theme	N-glycosylated	1121:1134	arg1	SARS-CoV-2					1145:1154	SARS-CoV-2	1145:1154	SARS-CoV-2	1145:1154	All 22 N-glycosylated sites of SARS-CoV-2 are modified by high-mannose N-glycans.
32518941	7	9	theme	N-glycosylated	1121:1134	arg1	sites					1136:1140	All 22 N-glycosylated sites	1114:1140	All 22 N-glycosylated sites of SARS-CoV-2	1114:1154	All 22 N-glycosylated sites of SARS-CoV-2 are modified by high-mannose N-glycans.
32518941	1	10	theme	sugar	221:225	arg1	coat					227:230	the sugar coat	217:230	the sugar coat on their spike glycoproteins	217:259	Coronaviruses hijack human enzymes to assemble the sugar coat on their spike glycoproteins.
32518941	6	11	theme	proteins	981:988	arg1	accessibility					958:970	the surface accessibility	946:970	the surface accessibility of spike proteins according to cryogenic electron microscopy and homolog-modeled structures and available antibodies that bind to SARS-CoV-1	946:1111	We further analyzed the surface accessibility of spike proteins according to cryogenic electron microscopy and homolog-modeled structures and available antibodies that bind to SARS-CoV-1.
32518941	9	12	theme	receptor-binding	1319:1334	arg1	SARS-CoV-2					1346:1355	SARS-CoV-2	1346:1355	SARS-CoV-2	1346:1355	Electron densities of glycans cover most of the spike receptor-binding domain of SARS-CoV-2, except YQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQ, similar to a region FSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQ in SARS-CoV-1.
32518941	9	12	theme	receptor-binding	1319:1334	arg1	domain					1336:1341	the spike receptor-binding domain	1309:1341	the spike receptor-binding domain of SARS-CoV-2	1309:1355	Electron densities of glycans cover most of the spike receptor-binding domain of SARS-CoV-2, except YQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQ, similar to a region FSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQ in SARS-CoV-1.
32518941	5	13	theme	spectrometry	835:846	arg1	spectrums					856:864	tandem mass spectrometry (MS/MS) spectrums	823:864	tandem mass spectrometry (MS/MS) spectrums for glycopeptides of all 22 predicted N-glycosylated sites	823:923	We acquired tandem mass spectrometry (MS/MS) spectrums for glycopeptides of all 22 predicted N-glycosylated sites.
32518941	1	14	from	coat	227:230	arg1	glycoproteins					247:259	their spike glycoproteins	235:259	their spike glycoproteins	235:259	Coronaviruses hijack human enzymes to assemble the sugar coat on their spike glycoproteins.
32518941	5	15	gly	N-glycosylated	904:917	arg1	sites					919:923	all 22 predicted N-glycosylated sites	887:923	all 22 predicted N-glycosylated sites	887:923	We acquired tandem mass spectrometry (MS/MS) spectrums for glycopeptides of all 22 predicted N-glycosylated sites.
32518941	3	16	dep	US	545:546	arg1	Food					548:551	Food	548:551	Food	548:551	Glycosylation by insect cells differs from the native form produced in human cells, but insect cell-derived influenza vaccines have been approved by the US Food and Drug Administration.
32518941	3	16	dep	US	545:546	arg1	Drug					557:560	Drug	557:560	Drug	557:560	Glycosylation by insect cells differs from the native form produced in human cells, but insect cell-derived influenza vaccines have been approved by the US Food and Drug Administration.
32518941	6	17	theme	surface	950:956	arg1	accessibility					958:970	the surface accessibility	946:970	the surface accessibility of spike proteins according to cryogenic electron microscopy and homolog-modeled structures and available antibodies that bind to SARS-CoV-1	946:1111	We further analyzed the surface accessibility of spike proteins according to cryogenic electron microscopy and homolog-modeled structures and available antibodies that bind to SARS-CoV-1.
32518941	11	18	theme	sugar	1684:1688	arg1	modification					1690:1701	sugar modification	1684:1701	sugar modification	1684:1701	Because the majority of antibody paratopes bind to the peptide portion with or without sugar modification, we propose a snake-catching model for predicted paratopes: a minimal length of peptide is first clamped by a paratope and sugar modifications close to the peptide either strengthen or do not hinder the binding.
32518941	6	19	theme	available	1068:1076	arg1	antibodies					1078:1087	available antibodies	1068:1087	available antibodies	1068:1087	We further analyzed the surface accessibility of spike proteins according to cryogenic electron microscopy and homolog-modeled structures and available antibodies that bind to SARS-CoV-1.
32518941	7	20	gly	N-glycosylated	1121:1134	arg1	SARS-CoV-2					1145:1154	SARS-CoV-2	1145:1154	SARS-CoV-2	1145:1154	All 22 N-glycosylated sites of SARS-CoV-2 are modified by high-mannose N-glycans.
32518941	7	20	gly	N-glycosylated	1121:1134	arg1	sites					1136:1140	All 22 N-glycosylated sites	1114:1140	All 22 N-glycosylated sites of SARS-CoV-2	1114:1154	All 22 N-glycosylated sites of SARS-CoV-2 are modified by high-mannose N-glycans.
32518941	11	21	theme	antibody	1621:1628	arg1	paratopes					1630:1638	antibody paratopes	1621:1638	antibody paratopes	1621:1638	Because the majority of antibody paratopes bind to the peptide portion with or without sugar modification, we propose a snake-catching model for predicted paratopes: a minimal length of peptide is first clamped by a paratope and sugar modifications close to the peptide either strengthen or do not hinder the binding.
32518941	0	22	theme	SARS-CoV-2	59:68	arg1	motifs					106:111	SARS-CoV-2 and accessible surface glycopeptide motifs	59:111	SARS-CoV-2 and accessible surface glycopeptide motifs	59:111	Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics.
32518941	5	23	theme	N-glycosylated	904:917	arg1	sites					919:923	all 22 predicted N-glycosylated sites	887:923	all 22 predicted N-glycosylated sites	887:923	We acquired tandem mass spectrometry (MS/MS) spectrums for glycopeptides of all 22 predicted N-glycosylated sites.
32518941	11	24	dep	paratope	1813:1820	arg1	a					1811:1811	a	1811:1811	a	1811:1811	Because the majority of antibody paratopes bind to the peptide portion with or without sugar modification, we propose a snake-catching model for predicted paratopes: a minimal length of peptide is first clamped by a paratope and sugar modifications close to the peptide either strengthen or do not hinder the binding.
32518941	4	25	theme	BTI-Tn-5B1-4	706:717	arg1	cells					726:730	BTI-Tn-5B1-4 insect cells	706:730	BTI-Tn-5B1-4 insect cells	706:730	In this study, we analyzed recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells, by trypsin and chymotrypsin digestion followed by mass spectrometry analysis.
32518941	0	26	theme	accessible	74:83	arg1	motifs					106:111	SARS-CoV-2 and accessible surface glycopeptide motifs	59:111	SARS-CoV-2 and accessible surface glycopeptide motifs	59:111	Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics.
32518941	1	27	theme	hijack	184:189	arg1	enzymes					197:203	Coronaviruses hijack human enzymes	170:203	Coronaviruses hijack human enzymes	170:203	Coronaviruses hijack human enzymes to assemble the sugar coat on their spike glycoproteins.
32518941	0	28	from	Identification	0:13	arg1	glycoprotein					43:54	spike glycoprotein	37:54	spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs	37:111	Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics.
32518941	0	29	theme	glycopeptide	93:104	arg1	motifs					106:111	SARS-CoV-2 and accessible surface glycopeptide motifs	59:111	SARS-CoV-2 and accessible surface glycopeptide motifs	59:111	Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics.
32518941	6	30	theme	electron	1013:1020	arg1	microscopy					1022:1031	cryogenic electron microscopy	1003:1031	cryogenic electron microscopy	1003:1031	We further analyzed the surface accessibility of spike proteins according to cryogenic electron microscopy and homolog-modeled structures and available antibodies that bind to SARS-CoV-1.
32518941	0	31	from	glycoprotein	43:54	arg1	Identification					0:13	Identification	0:13	Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics.	0:168	Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics.
32518941	4	32	theme	spectrometry	788:799	arg1	analysis					801:808	mass spectrometry analysis	783:808	mass spectrometry analysis	783:808	In this study, we analyzed recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells, by trypsin and chymotrypsin digestion followed by mass spectrometry analysis.
32518941	3	33	theme	native	439:444	arg1	form					446:449	the native form	435:449	the native form produced in human cells	435:473	Glycosylation by insect cells differs from the native form produced in human cells, but insect cell-derived influenza vaccines have been approved by the US Food and Drug Administration.
32518941	5	34	theme	mass	830:833	arg1	MS/MS					849:853	MS/MS	849:853	MS/MS	849:853	We acquired tandem mass spectrometry (MS/MS) spectrums for glycopeptides of all 22 predicted N-glycosylated sites.
32518941	5	34	theme	mass	830:833	arg1	spectrometry					835:846	tandem mass spectrometry	823:846	tandem mass spectrometry (MS/MS) spectrums for glycopeptides of all 22 predicted N-glycosylated sites	823:923	We acquired tandem mass spectrometry (MS/MS) spectrums for glycopeptides of all 22 predicted N-glycosylated sites.
32518941	11	35	theme	sugar	1826:1830	arg1	modifications					1832:1844	sugar modifications	1826:1844	sugar modifications	1826:1844	Because the majority of antibody paratopes bind to the peptide portion with or without sugar modification, we propose a snake-catching model for predicted paratopes: a minimal length of peptide is first clamped by a paratope and sugar modifications close to the peptide either strengthen or do not hinder the binding.
32518941	3	36	theme	human	463:467	arg1	cells					469:473	human cells	463:473	human cells	463:473	Glycosylation by insect cells differs from the native form produced in human cells, but insect cell-derived influenza vaccines have been approved by the US Food and Drug Administration.
32518941	10	37	theme	Other	1470:1474	arg1	domains					1492:1498	Other surface-exposed domains	1470:1498	Other surface-exposed domains	1470:1498	Other surface-exposed domains include those located on central helix, connecting region, heptad repeats and N-terminal domain.
32518941	3	38	theme	influenza	500:508	arg1	vaccines					510:517	insect cell-derived influenza vaccines	480:517	insect cell-derived influenza vaccines	480:517	Glycosylation by insect cells differs from the native form produced in human cells, but insect cell-derived influenza vaccines have been approved by the US Food and Drug Administration.
32518941	11	39	dep	clamped	1800:1806	arg1	either					1867:1872	either	1867:1872	either	1867:1872	Because the majority of antibody paratopes bind to the peptide portion with or without sugar modification, we propose a snake-catching model for predicted paratopes: a minimal length of peptide is first clamped by a paratope and sugar modifications close to the peptide either strengthen or do not hinder the binding.
32518941	7	40	mod	modified	1160:1167	arg1	SARS-CoV-2					1145:1154	SARS-CoV-2	1145:1154	SARS-CoV-2	1145:1154	All 22 N-glycosylated sites of SARS-CoV-2 are modified by high-mannose N-glycans.
32518941	7	40	mod	modified	1160:1167	arg3	N-glycans					1185:1193	high-mannose N-glycans	1172:1193	high-mannose N-glycans	1172:1193	All 22 N-glycosylated sites of SARS-CoV-2 are modified by high-mannose N-glycans.
32518941	7	40	mod	modified	1160:1167	arg1	sites					1136:1140	All 22 N-glycosylated sites	1114:1140	All 22 N-glycosylated sites of SARS-CoV-2	1114:1154	All 22 N-glycosylated sites of SARS-CoV-2 are modified by high-mannose N-glycans.
32518941	0	41	gly	N-glycosites	21:32	arg2	N-glycosites					21:32	22 N-glycosites	18:32	22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs	18:111	Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics.
32518941	10	42	theme	heptad	1559:1564	arg1	repeats					1566:1572	heptad repeats	1559:1572	heptad repeats	1559:1572	Other surface-exposed domains include those located on central helix, connecting region, heptad repeats and N-terminal domain.
32518941	11	43	theme	snake-catching	1717:1730	arg1	model					1732:1736	a snake-catching model	1715:1736	a snake-catching model for predicted paratopes	1715:1760	Because the majority of antibody paratopes bind to the peptide portion with or without sugar modification, we propose a snake-catching model for predicted paratopes: a minimal length of peptide is first clamped by a paratope and sugar modifications close to the peptide either strengthen or do not hinder the binding.
32518941	3	44	theme	insect	480:485	arg1	vaccines					510:517	insect cell-derived influenza vaccines	480:517	insect cell-derived influenza vaccines	480:517	Glycosylation by insect cells differs from the native form produced in human cells, but insect cell-derived influenza vaccines have been approved by the US Food and Drug Administration.
32518941	0	45	dep	Identification	0:13	arg1	Implications					114:125	Implications	114:125	Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics.	0:168	Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics.
32518941	4	46	theme	severe	617:622	arg1	protein					684:690	recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein	605:690	recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells	605:730	In this study, we analyzed recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells, by trypsin and chymotrypsin digestion followed by mass spectrometry analysis.
32518941	11	47	theme	peptide	1783:1789	arg1	length					1773:1778	a minimal length	1763:1778	a minimal length of peptide	1763:1789	Because the majority of antibody paratopes bind to the peptide portion with or without sugar modification, we propose a snake-catching model for predicted paratopes: a minimal length of peptide is first clamped by a paratope and sugar modifications close to the peptide either strengthen or do not hinder the binding.
32518941	7	48	theme	high-mannose	1172:1183	arg1	N-glycans					1185:1193	high-mannose N-glycans	1172:1193	high-mannose N-glycans	1172:1193	All 22 N-glycosylated sites of SARS-CoV-2 are modified by high-mannose N-glycans.
32518941	5	49	gly	glycopeptides	870:882	arg1	sites					919:923	all 22 predicted N-glycosylated sites	887:923	all 22 predicted N-glycosylated sites	887:923	We acquired tandem mass spectrometry (MS/MS) spectrums for glycopeptides of all 22 predicted N-glycosylated sites.
32518941	5	49	gly	glycopeptides	870:882	arg2	glycopeptides					870:882	glycopeptides	870:882	glycopeptides of all 22 predicted N-glycosylated sites	870:923	We acquired tandem mass spectrometry (MS/MS) spectrums for glycopeptides of all 22 predicted N-glycosylated sites.
32518941	9	50	theme	Electron	1265:1272	arg1	densities					1274:1282	Electron densities	1265:1282	Electron densities of glycans	1265:1293	Electron densities of glycans cover most of the spike receptor-binding domain of SARS-CoV-2, except YQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQ, similar to a region FSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQ in SARS-CoV-1.
32518941	5	51	theme	sites	919:923	arg1	glycopeptides					870:882	glycopeptides	870:882	glycopeptides of all 22 predicted N-glycosylated sites	870:923	We acquired tandem mass spectrometry (MS/MS) spectrums for glycopeptides of all 22 predicted N-glycosylated sites.
32518941	7	52	theme	SARS-CoV-2	1145:1154	arg1	SARS-CoV-2					1145:1154	SARS-CoV-2	1145:1154	SARS-CoV-2	1145:1154	All 22 N-glycosylated sites of SARS-CoV-2 are modified by high-mannose N-glycans.
32518941	7	52	theme	SARS-CoV-2	1145:1154	arg1	sites					1136:1140	All 22 N-glycosylated sites	1114:1140	All 22 N-glycosylated sites of SARS-CoV-2	1114:1154	All 22 N-glycosylated sites of SARS-CoV-2 are modified by high-mannose N-glycans.
32518941	1	53	theme	human	191:195	arg1	enzymes					197:203	Coronaviruses hijack human enzymes	170:203	Coronaviruses hijack human enzymes	170:203	Coronaviruses hijack human enzymes to assemble the sugar coat on their spike glycoproteins.
32518941	8	54	theme	glycopeptide	1240:1251	arg1	identities					1253:1262	the glycopeptide identities	1236:1262	the glycopeptide identities	1236:1262	MS/MS fragmentation clearly established the glycopeptide identities.
32518941	2	55	theme	peptide	337:343	arg1	epitopes					345:352	the antigenic viral peptide epitopes	317:352	the antigenic viral peptide epitopes hidden by the sugar coat	317:377	The mechanisms by which human antibodies may recognize the antigenic viral peptide epitopes hidden by the sugar coat are unknown.
32518941	6	56	theme	cryogenic	1003:1011	arg1	microscopy					1022:1031	cryogenic electron microscopy	1003:1031	cryogenic electron microscopy	1003:1031	We further analyzed the surface accessibility of spike proteins according to cryogenic electron microscopy and homolog-modeled structures and available antibodies that bind to SARS-CoV-1.
32518941	2	57	theme	antigenic	321:329	arg1	epitopes					345:352	the antigenic viral peptide epitopes	317:352	the antigenic viral peptide epitopes hidden by the sugar coat	317:377	The mechanisms by which human antibodies may recognize the antigenic viral peptide epitopes hidden by the sugar coat are unknown.
32518941	4	58	theme	respiratory	630:640	arg1	protein					684:690	recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein	605:690	recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells	605:730	In this study, we analyzed recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells, by trypsin and chymotrypsin digestion followed by mass spectrometry analysis.
32518941	0	59	theme	antibody	147:154	arg1	therapeutics					156:167	antibody therapeutics	147:167	antibody therapeutics	147:167	Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics.
32518941	9	60	theme	spike	1313:1317	arg1	SARS-CoV-2					1346:1355	SARS-CoV-2	1346:1355	SARS-CoV-2	1346:1355	Electron densities of glycans cover most of the spike receptor-binding domain of SARS-CoV-2, except YQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQ, similar to a region FSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQ in SARS-CoV-1.
32518941	9	60	theme	spike	1313:1317	arg1	domain					1336:1341	the spike receptor-binding domain	1309:1341	the spike receptor-binding domain of SARS-CoV-2	1309:1355	Electron densities of glycans cover most of the spike receptor-binding domain of SARS-CoV-2, except YQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQ, similar to a region FSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQ in SARS-CoV-1.
32518941	6	61	theme	spike	975:979	arg1	proteins					981:988	spike proteins	975:988	spike proteins	975:988	We further analyzed the surface accessibility of spike proteins according to cryogenic electron microscopy and homolog-modeled structures and available antibodies that bind to SARS-CoV-1.
32518941	4	62	theme	coronavirus	651:661	arg1	protein					684:690	recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein	605:690	recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells	605:730	In this study, we analyzed recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells, by trypsin and chymotrypsin digestion followed by mass spectrometry analysis.
32518941	3	63	link	cell-derived	487:498	arg1	vaccines					510:517	insect cell-derived influenza vaccines	480:517	insect cell-derived influenza vaccines	480:517	Glycosylation by insect cells differs from the native form produced in human cells, but insect cell-derived influenza vaccines have been approved by the US Food and Drug Administration.
32518941	4	64	theme	insect	719:724	arg1	cells					726:730	BTI-Tn-5B1-4 insect cells	706:730	BTI-Tn-5B1-4 insect cells	706:730	In this study, we analyzed recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells, by trypsin and chymotrypsin digestion followed by mass spectrometry analysis.
32518941	11	65	theme	paratopes	1630:1638	arg1	majority					1609:1616	the majority	1605:1616	the majority of antibody paratopes	1605:1638	Because the majority of antibody paratopes bind to the peptide portion with or without sugar modification, we propose a snake-catching model for predicted paratopes: a minimal length of peptide is first clamped by a paratope and sugar modifications close to the peptide either strengthen or do not hinder the binding.
32518941	9	66	theme	SARS-CoV-2	1346:1355	arg1	SARS-CoV-2					1346:1355	SARS-CoV-2	1346:1355	SARS-CoV-2	1346:1355	Electron densities of glycans cover most of the spike receptor-binding domain of SARS-CoV-2, except YQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQ, similar to a region FSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQ in SARS-CoV-1.
32518941	9	66	theme	SARS-CoV-2	1346:1355	arg1	domain					1336:1341	the spike receptor-binding domain	1309:1341	the spike receptor-binding domain of SARS-CoV-2	1309:1355	Electron densities of glycans cover most of the spike receptor-binding domain of SARS-CoV-2, except YQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQ, similar to a region FSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQ in SARS-CoV-1.
32518941	3	67	dep	Food	548:551	arg1	Administration					562:575	Administration	562:575	Administration	562:575	Glycosylation by insect cells differs from the native form produced in human cells, but insect cell-derived influenza vaccines have been approved by the US Food and Drug Administration.
32518941	5	68	theme	predicted	894:902	arg1	sites					919:923	all 22 predicted N-glycosylated sites	887:923	all 22 predicted N-glycosylated sites	887:923	We acquired tandem mass spectrometry (MS/MS) spectrums for glycopeptides of all 22 predicted N-glycosylated sites.
32518941	0	69	from	N-glycosites	21:32	arg1	glycoprotein					43:54	spike glycoprotein	37:54	spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs	37:111	Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics.
32518941	11	70	theme	peptide	1652:1658	arg1	portion					1660:1666	the peptide portion	1648:1666	the peptide portion	1648:1666	Because the majority of antibody paratopes bind to the peptide portion with or without sugar modification, we propose a snake-catching model for predicted paratopes: a minimal length of peptide is first clamped by a paratope and sugar modifications close to the peptide either strengthen or do not hinder the binding.
32518941	0	71	theme	surface	85:91	arg1	motifs					106:111	SARS-CoV-2 and accessible surface glycopeptide motifs	59:111	SARS-CoV-2 and accessible surface glycopeptide motifs	59:111	Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics.
32518941	1	72	theme	Coronaviruses	170:182	arg1	enzymes					197:203	Coronaviruses hijack human enzymes	170:203	Coronaviruses hijack human enzymes	170:203	Coronaviruses hijack human enzymes to assemble the sugar coat on their spike glycoproteins.
32518941	6	73	theme	homolog-modeled	1037:1051	arg1	structures					1053:1062	homolog-modeled structures	1037:1062	homolog-modeled structures	1037:1062	We further analyzed the surface accessibility of spike proteins according to cryogenic electron microscopy and homolog-modeled structures and available antibodies that bind to SARS-CoV-1.
32518941	10	74	theme	N-terminal	1578:1587	arg1	domain					1589:1594	N-terminal domain	1578:1594	N-terminal domain	1578:1594	Other surface-exposed domains include those located on central helix, connecting region, heptad repeats and N-terminal domain.
32518941	0	75	theme	motifs	106:111	arg1	glycoprotein					43:54	spike glycoprotein	37:54	spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs	37:111	Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics.
32518941	1	76	gly	glycoproteins	247:259	arg1	glycoproteins					247:259	their spike glycoproteins	235:259	their spike glycoproteins	235:259	Coronaviruses hijack human enzymes to assemble the sugar coat on their spike glycoproteins.
32518941	4	77	theme	mass	783:786	arg1	analysis					801:808	mass spectrometry analysis	783:808	mass spectrometry analysis	783:808	In this study, we analyzed recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells, by trypsin and chymotrypsin digestion followed by mass spectrometry analysis.
32518941	5	78	theme	tandem	823:828	arg1	MS/MS					849:853	MS/MS	849:853	MS/MS	849:853	We acquired tandem mass spectrometry (MS/MS) spectrums for glycopeptides of all 22 predicted N-glycosylated sites.
32518941	5	78	theme	tandem	823:828	arg1	spectrometry					835:846	tandem mass spectrometry	823:846	tandem mass spectrometry (MS/MS) spectrums for glycopeptides of all 22 predicted N-glycosylated sites	823:923	We acquired tandem mass spectrometry (MS/MS) spectrums for glycopeptides of all 22 predicted N-glycosylated sites.
32518941	0	79	gly	glycopeptide	93:104	arg2	glycopeptide					93:104	SARS-CoV-2 and accessible surface glycopeptide motifs	59:111	SARS-CoV-2 and accessible surface glycopeptide motifs	59:111	Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics.
32518941	3	80	theme	insect	409:414	arg1	cells					416:420	insect cells	409:420	insect cells	409:420	Glycosylation by insect cells differs from the native form produced in human cells, but insect cell-derived influenza vaccines have been approved by the US Food and Drug Administration.
32518941	4	81	theme	trypsin	736:742	arg1	digestion					761:769	trypsin and chymotrypsin digestion	736:769	trypsin and chymotrypsin digestion followed by mass spectrometry analysis	736:808	In this study, we analyzed recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells, by trypsin and chymotrypsin digestion followed by mass spectrometry analysis.
32518941	0	82	theme	N-glycosites	21:32	arg1	Identification					0:13	Identification	0:13	Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics.	0:168	Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics.
32518941	4	83	theme	chymotrypsin	748:759	arg1	digestion					761:769	trypsin and chymotrypsin digestion	736:769	trypsin and chymotrypsin digestion followed by mass spectrometry analysis	736:808	In this study, we analyzed recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells, by trypsin and chymotrypsin digestion followed by mass spectrometry analysis.
32518941	0	84	theme	spike	37:41	arg1	glycoprotein					43:54	spike glycoprotein	37:54	spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs	37:111	Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics.
32518941	10	85	theme	surface-exposed	1476:1490	arg1	domains					1492:1498	Other surface-exposed domains	1470:1498	Other surface-exposed domains	1470:1498	Other surface-exposed domains include those located on central helix, connecting region, heptad repeats and N-terminal domain.
32518941	11	86	theme	predicted	1742:1750	arg1	paratopes					1752:1760	predicted paratopes	1742:1760	predicted paratopes	1742:1760	Because the majority of antibody paratopes bind to the peptide portion with or without sugar modification, we propose a snake-catching model for predicted paratopes: a minimal length of peptide is first clamped by a paratope and sugar modifications close to the peptide either strengthen or do not hinder the binding.
32518941	4	87	theme	recombinant	605:615	arg1	protein					684:690	recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein	605:690	recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells	605:730	In this study, we analyzed recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells, by trypsin and chymotrypsin digestion followed by mass spectrometry analysis.
32518941	3	88	theme	cell-derived	487:498	arg1	vaccines					510:517	insect cell-derived influenza vaccines	480:517	insect cell-derived influenza vaccines	480:517	Glycosylation by insect cells differs from the native form produced in human cells, but insect cell-derived influenza vaccines have been approved by the US Food and Drug Administration.
32518941	1	89	theme	spike	241:245	arg1	glycoproteins					247:259	their spike glycoproteins	235:259	their spike glycoproteins	235:259	Coronaviruses hijack human enzymes to assemble the sugar coat on their spike glycoproteins.
32518941	8	90	theme	MS/MS	1196:1200	arg1	fragmentation					1202:1214	MS/MS fragmentation	1196:1214	MS/MS fragmentation	1196:1214	MS/MS fragmentation clearly established the glycopeptide identities.
32518941	0	91	gly	glycoprotein	43:54	arg1	glycoprotein					43:54	spike glycoprotein	37:54	spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs	37:111	Identification of 22 N-glycosites on spike glycoprotein of SARS-CoV-2 and accessible surface glycopeptide motifs: Implications for vaccination and antibody therapeutics.
32518941	4	92	theme	acute	624:628	arg1	protein					684:690	recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein	605:690	recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells	605:730	In this study, we analyzed recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein secreted from BTI-Tn-5B1-4 insect cells, by trypsin and chymotrypsin digestion followed by mass spectrometry analysis.
32518941	8	93	gly	glycopeptide	1240:1251	arg2	glycopeptide					1240:1251	glycopeptide	1240:1251	glycopeptide	1240:1251	MS/MS fragmentation clearly established the glycopeptide identities.
32663557	8	0	theme	swelling	843:850	arg1	%					863:863	12.0%	859:863	12.0%	859:863	NSY starch had higher gelatinization temperature (71.5 °C), enthalpy (14.14 J/g), peak viscosity (8590 cP) and swelling power (12.0%) than TSY and HSY.
32663557	8	0	theme	swelling	843:850	arg1	power					852:856	swelling power	843:856	swelling power (12.0%)	843:864	NSY starch had higher gelatinization temperature (71.5 °C), enthalpy (14.14 J/g), peak viscosity (8590 cP) and swelling power (12.0%) than TSY and HSY.
32663557	8	1	theme	higher	747:752	arg1	71.5 °C					782:788	71.5 °C	782:788	71.5 °C	782:788	NSY starch had higher gelatinization temperature (71.5 °C), enthalpy (14.14 J/g), peak viscosity (8590 cP) and swelling power (12.0%) than TSY and HSY.
32663557	8	1	theme	higher	747:752	arg1	temperature					769:779	higher gelatinization temperature	747:779	higher gelatinization temperature (71.5 °C)	747:789	NSY starch had higher gelatinization temperature (71.5 °C), enthalpy (14.14 J/g), peak viscosity (8590 cP) and swelling power (12.0%) than TSY and HSY.
32663557	0	2	theme	opposita	80:87	arg1	Thunb					89:93	Dioscorea opposita Thunb.	70:94	Dioscorea opposita Thunb.	70:94	Physicochemical and structural properties of low-amylose Chinese yam (Dioscorea opposita Thunb.)
32663557	0	2	theme	opposita	80:87	arg1	yam					65:67	low-amylose Chinese yam	45:67	low-amylose Chinese yam (Dioscorea opposita Thunb.)	45:95	Physicochemical and structural properties of low-amylose Chinese yam (Dioscorea opposita Thunb.)
32663557	4	3	theme	starches	343:350	arg1	properties					325:334	thermal properties	317:334	thermal properties	317:334	The composition, physicochemical properties, morphology, and thermal properties of the starches from these cultivars were investigated in this study.
32663557	4	3	theme	starches	343:350	arg1	morphology					301:310	morphology	301:310	morphology	301:310	The composition, physicochemical properties, morphology, and thermal properties of the starches from these cultivars were investigated in this study.
32663557	4	3	theme	starches	343:350	arg1	composition					260:270	The composition	256:270	The composition	256:270	The composition, physicochemical properties, morphology, and thermal properties of the starches from these cultivars were investigated in this study.
32663557	4	3	theme	starches	343:350	arg1	properties					289:298	physicochemical properties	273:298	physicochemical properties	273:298	The composition, physicochemical properties, morphology, and thermal properties of the starches from these cultivars were investigated in this study.
32663557	6	4	theme	starches	531:538	arg1	diameter					515:522	The average particle diameter	494:522	The average particle diameter of the starches	494:538	The average particle diameter of the starches ranged from 25.83 to 28.93 μm.
32663557	12	5	theme	physicochemical	1351:1365	arg1	properties					1382:1391	These specific physicochemical and structural properties	1336:1391	These specific physicochemical and structural properties	1336:1391	These specific physicochemical and structural properties indicated the industrial potential of low-amylose yam starch.
32663557	7	6	theme	molecular	628:636	arg1	Mn					647:648	Mn	647:648	Mn	647:648	Weight-average molecular weights (Mw) and number-average molecular weights (Mn) ranged from 1.29 to 1.84 × 105 g/mol and 5.93 to 8.36 × 104 g/mol, respectively.
32663557	7	6	theme	molecular	628:636	arg1	weights					638:644	number-average molecular weights	613:644	number-average molecular weights (Mn)	613:649	Weight-average molecular weights (Mw) and number-average molecular weights (Mn) ranged from 1.29 to 1.84 × 105 g/mol and 5.93 to 8.36 × 104 g/mol, respectively.
32663557	10	7	theme	amylose	1196:1202	arg1	content					1204:1210	amylose content	1196:1210	amylose content	1196:1210	To, Tp, ΔH, peak viscosity and swelling power at 95 °C were significantly correlated to amylose content.
32663557	11	8	contain	have	1253:1256	arg1	starch					1233:1238	The low-amylose NSY starch	1213:1238	The low-amylose NSY starch	1213:1238	The low-amylose NSY starch was found to have high gelatinization temperature, enthalpy, peak viscosity and swelling power.
32663557	11	8	contain	have	1253:1256	arg2	enthalpy					1291:1298	enthalpy	1291:1298	enthalpy	1291:1298	The low-amylose NSY starch was found to have high gelatinization temperature, enthalpy, peak viscosity and swelling power.
32663557	11	8	contain	have	1253:1256	arg2	power					1329:1333	swelling power	1320:1333	swelling power	1320:1333	The low-amylose NSY starch was found to have high gelatinization temperature, enthalpy, peak viscosity and swelling power.
32663557	11	8	contain	have	1253:1256	arg2	viscosity					1306:1314	peak viscosity	1301:1314	peak viscosity	1301:1314	The low-amylose NSY starch was found to have high gelatinization temperature, enthalpy, peak viscosity and swelling power.
32663557	11	8	contain	have	1253:1256	arg2	temperature					1278:1288	high gelatinization temperature	1258:1288	high gelatinization temperature	1258:1288	The low-amylose NSY starch was found to have high gelatinization temperature, enthalpy, peak viscosity and swelling power.
32663557	9	9	theme	yam	975:977	arg1	starches					979:986	Chinese yam starches	967:986	Chinese yam starches	967:986	X-ray diffraction (XRD) and Fourier transform infrared (FTIR) tests indicated that Chinese yam starches had CB-type crystalline structure with crystallinity ranging from 21.91% to 27.08% and a short-range ordered structure.
32663557	11	10	theme	high	1258:1261	arg1	temperature					1278:1288	high gelatinization temperature	1258:1288	high gelatinization temperature	1258:1288	The low-amylose NSY starch was found to have high gelatinization temperature, enthalpy, peak viscosity and swelling power.
32663557	12	11	theme	structural	1371:1380	arg1	properties					1382:1391	These specific physicochemical and structural properties	1336:1391	These specific physicochemical and structural properties	1336:1391	These specific physicochemical and structural properties indicated the industrial potential of low-amylose yam starch.
32663557	11	12	theme	NSY	1229:1231	arg1	starch					1233:1238	The low-amylose NSY starch	1213:1238	The low-amylose NSY starch	1213:1238	The low-amylose NSY starch was found to have high gelatinization temperature, enthalpy, peak viscosity and swelling power.
32663557	9	13	theme	infrared	930:937	arg1	tests					946:950	infrared (FTIR) tests	930:950	infrared (FTIR) tests	930:950	X-ray diffraction (XRD) and Fourier transform infrared (FTIR) tests indicated that Chinese yam starches had CB-type crystalline structure with crystallinity ranging from 21.91% to 27.08% and a short-range ordered structure.
32663557	4	14	theme	thermal	317:323	arg1	properties					325:334	thermal properties	317:334	thermal properties	317:334	The composition, physicochemical properties, morphology, and thermal properties of the starches from these cultivars were investigated in this study.
32663557	6	15	theme	average	498:504	arg1	diameter					515:522	The average particle diameter	494:522	The average particle diameter of the starches	494:538	The average particle diameter of the starches ranged from 25.83 to 28.93 μm.
32663557	9	16	dep	Fourier	912:918	arg1	transform					920:928	transform	920:928	transform infrared (FTIR) tests	920:950	X-ray diffraction (XRD) and Fourier transform infrared (FTIR) tests indicated that Chinese yam starches had CB-type crystalline structure with crystallinity ranging from 21.91% to 27.08% and a short-range ordered structure.
32663557	0	17	theme	Physicochemical	0:14	arg1	properties					31:40	Physicochemical and structural properties	0:40	Physicochemical and structural properties of low-amylose Chinese yam (Dioscorea opposita Thunb.)	0:95	Physicochemical and structural properties of low-amylose Chinese yam (Dioscorea opposita Thunb.)
32663557	9	18	contain	had	988:990	arg1	starches					979:986	Chinese yam starches	967:986	Chinese yam starches	967:986	X-ray diffraction (XRD) and Fourier transform infrared (FTIR) tests indicated that Chinese yam starches had CB-type crystalline structure with crystallinity ranging from 21.91% to 27.08% and a short-range ordered structure.
32663557	9	18	contain	had	988:990	arg2	structure					1012:1020	CB-type crystalline structure	992:1020	CB-type crystalline structure	992:1020	X-ray diffraction (XRD) and Fourier transform infrared (FTIR) tests indicated that Chinese yam starches had CB-type crystalline structure with crystallinity ranging from 21.91% to 27.08% and a short-range ordered structure.
32663557	4	19	from	properties	325:334	arg1	cultivars					363:371	these cultivars	357:371	these cultivars	357:371	The composition, physicochemical properties, morphology, and thermal properties of the starches from these cultivars were investigated in this study.
32663557	10	20	theme	swelling	1139:1146	arg1	power					1148:1152	swelling power	1139:1152	swelling power	1139:1152	To, Tp, ΔH, peak viscosity and swelling power at 95 °C were significantly correlated to amylose content.
32663557	10	21	from	95 °C	1157:1161	arg1	ΔH					1116:1117	ΔH	1116:1117	ΔH	1116:1117	To, Tp, ΔH, peak viscosity and swelling power at 95 °C were significantly correlated to amylose content.
32663557	10	21	from	95 °C	1157:1161	arg1	viscosity					1125:1133	peak viscosity	1120:1133	peak viscosity	1120:1133	To, Tp, ΔH, peak viscosity and swelling power at 95 °C were significantly correlated to amylose content.
32663557	10	21	from	95 °C	1157:1161	arg1	Tp					1112:1113	Tp	1112:1113	Tp	1112:1113	To, Tp, ΔH, peak viscosity and swelling power at 95 °C were significantly correlated to amylose content.
32663557	10	21	from	95 °C	1157:1161	arg1	power					1148:1152	swelling power	1139:1152	swelling power	1139:1152	To, Tp, ΔH, peak viscosity and swelling power at 95 °C were significantly correlated to amylose content.
32663557	6	22	theme	particle	506:513	arg1	diameter					515:522	The average particle diameter	494:522	The average particle diameter of the starches	494:538	The average particle diameter of the starches ranged from 25.83 to 28.93 μm.
32663557	2	23	theme	main	175:178	arg1	NSY					119:121	NSY	119:121	NSY	119:121	Nuoshanyao (NSY), Tiegunshanyao (TSY) and Huaishanyao (HSY) are the main cultivars of Chinese yam (Dioscorea opposita Thunb.)
32663557	2	23	theme	main	175:178	arg1	cultivars					180:188	the main cultivars	171:188	the main cultivars of Chinese yam	171:203	Nuoshanyao (NSY), Tiegunshanyao (TSY) and Huaishanyao (HSY) are the main cultivars of Chinese yam (Dioscorea opposita Thunb.)
32663557	2	23	theme	main	175:178	arg1	HSY					162:164	HSY	162:164	HSY	162:164	Nuoshanyao (NSY), Tiegunshanyao (TSY) and Huaishanyao (HSY) are the main cultivars of Chinese yam (Dioscorea opposita Thunb.)
32663557	2	23	theme	main	175:178	arg1	TSY					140:142	TSY	140:142	TSY	140:142	Nuoshanyao (NSY), Tiegunshanyao (TSY) and Huaishanyao (HSY) are the main cultivars of Chinese yam (Dioscorea opposita Thunb.)
32663557	2	24	dep	cultivars	180:188	arg1	Thunb					225:229	Thunb	225:229	Thunb	225:229	Nuoshanyao (NSY), Tiegunshanyao (TSY) and Huaishanyao (HSY) are the main cultivars of Chinese yam (Dioscorea opposita Thunb.)
32663557	0	25	theme	structural	20:29	arg1	properties					31:40	Physicochemical and structural properties	0:40	Physicochemical and structural properties of low-amylose Chinese yam (Dioscorea opposita Thunb.)	0:95	Physicochemical and structural properties of low-amylose Chinese yam (Dioscorea opposita Thunb.)
32663557	11	26	theme	swelling	1320:1327	arg1	power					1329:1333	swelling power	1320:1333	swelling power	1320:1333	The low-amylose NSY starch was found to have high gelatinization temperature, enthalpy, peak viscosity and swelling power.
32663557	0	27	theme	low-amylose	45:55	arg1	Thunb					89:93	Dioscorea opposita Thunb.	70:94	Dioscorea opposita Thunb.	70:94	Physicochemical and structural properties of low-amylose Chinese yam (Dioscorea opposita Thunb.)
32663557	0	27	theme	low-amylose	45:55	arg1	yam					65:67	low-amylose Chinese yam	45:67	low-amylose Chinese yam (Dioscorea opposita Thunb.)	45:95	Physicochemical and structural properties of low-amylose Chinese yam (Dioscorea opposita Thunb.)
32663557	11	28	theme	gelatinization	1263:1276	arg1	temperature					1278:1288	high gelatinization temperature	1258:1288	high gelatinization temperature	1258:1288	The low-amylose NSY starch was found to have high gelatinization temperature, enthalpy, peak viscosity and swelling power.
32663557	7	29	theme	number-average	613:626	arg1	Mn					647:648	Mn	647:648	Mn	647:648	Weight-average molecular weights (Mw) and number-average molecular weights (Mn) ranged from 1.29 to 1.84 × 105 g/mol and 5.93 to 8.36 × 104 g/mol, respectively.
32663557	7	29	theme	number-average	613:626	arg1	weights					638:644	number-average molecular weights	613:644	number-average molecular weights (Mn)	613:649	Weight-average molecular weights (Mw) and number-average molecular weights (Mn) ranged from 1.29 to 1.84 × 105 g/mol and 5.93 to 8.36 × 104 g/mol, respectively.
32663557	2	30	theme	Chinese	193:199	arg1	yam					201:203	Chinese yam	193:203	Chinese yam	193:203	Nuoshanyao (NSY), Tiegunshanyao (TSY) and Huaishanyao (HSY) are the main cultivars of Chinese yam (Dioscorea opposita Thunb.)
32663557	10	31	theme	peak	1120:1123	arg1	viscosity					1125:1133	peak viscosity	1120:1133	peak viscosity	1120:1133	To, Tp, ΔH, peak viscosity and swelling power at 95 °C were significantly correlated to amylose content.
32663557	12	32	theme	low-amylose	1431:1441	arg1	starch					1447:1452	low-amylose yam starch	1431:1452	low-amylose yam starch	1431:1452	These specific physicochemical and structural properties indicated the industrial potential of low-amylose yam starch.
32663557	9	33	theme	short-range	1077:1087	arg1	structure					1097:1105	a short-range ordered structure	1075:1105	a short-range ordered structure	1075:1105	X-ray diffraction (XRD) and Fourier transform infrared (FTIR) tests indicated that Chinese yam starches had CB-type crystalline structure with crystallinity ranging from 21.91% to 27.08% and a short-range ordered structure.
32663557	4	34	from	morphology	301:310	arg1	cultivars					363:371	these cultivars	357:371	these cultivars	357:371	The composition, physicochemical properties, morphology, and thermal properties of the starches from these cultivars were investigated in this study.
32663557	5	35	theme	other	464:468	arg1	cultivars					470:478	other cultivars	464:478	other cultivars (33.4-34.5%)	464:491	NSY starch (17.0%) was much lower in amylose content than other cultivars (33.4-34.5%).
32663557	5	35	theme	other	464:468	arg1	%					490:490	33.4-34.5%	481:490	33.4-34.5%	481:490	NSY starch (17.0%) was much lower in amylose content than other cultivars (33.4-34.5%).
32663557	9	36	theme	crystalline	1000:1010	arg1	structure					1012:1020	CB-type crystalline structure	992:1020	CB-type crystalline structure	992:1020	X-ray diffraction (XRD) and Fourier transform infrared (FTIR) tests indicated that Chinese yam starches had CB-type crystalline structure with crystallinity ranging from 21.91% to 27.08% and a short-range ordered structure.
32663557	0	37	theme	yam	65:67	arg1	properties					31:40	Physicochemical and structural properties	0:40	Physicochemical and structural properties of low-amylose Chinese yam (Dioscorea opposita Thunb.)	0:95	Physicochemical and structural properties of low-amylose Chinese yam (Dioscorea opposita Thunb.)
32663557	9	38	theme	ordered	1089:1095	arg1	structure					1097:1105	a short-range ordered structure	1075:1105	a short-range ordered structure	1075:1105	X-ray diffraction (XRD) and Fourier transform infrared (FTIR) tests indicated that Chinese yam starches had CB-type crystalline structure with crystallinity ranging from 21.91% to 27.08% and a short-range ordered structure.
32663557	8	39	theme	peak	814:817	arg1	8590 cP					830:836	8590 cP	830:836	8590 cP	830:836	NSY starch had higher gelatinization temperature (71.5 °C), enthalpy (14.14 J/g), peak viscosity (8590 cP) and swelling power (12.0%) than TSY and HSY.
32663557	8	39	theme	peak	814:817	arg1	viscosity					819:827	peak viscosity	814:827	peak viscosity (8590 cP)	814:837	NSY starch had higher gelatinization temperature (71.5 °C), enthalpy (14.14 J/g), peak viscosity (8590 cP) and swelling power (12.0%) than TSY and HSY.
32663557	0	40	theme	Chinese	57:63	arg1	Thunb					89:93	Dioscorea opposita Thunb.	70:94	Dioscorea opposita Thunb.	70:94	Physicochemical and structural properties of low-amylose Chinese yam (Dioscorea opposita Thunb.)
32663557	0	40	theme	Chinese	57:63	arg1	yam					65:67	low-amylose Chinese yam	45:67	low-amylose Chinese yam (Dioscorea opposita Thunb.)	45:95	Physicochemical and structural properties of low-amylose Chinese yam (Dioscorea opposita Thunb.)
32663557	12	41	theme	starch	1447:1452	arg1	potential					1418:1426	the industrial potential	1403:1426	the industrial potential of low-amylose yam starch	1403:1452	These specific physicochemical and structural properties indicated the industrial potential of low-amylose yam starch.
32663557	4	42	from	properties	289:298	arg1	cultivars					363:371	these cultivars	357:371	these cultivars	357:371	The composition, physicochemical properties, morphology, and thermal properties of the starches from these cultivars were investigated in this study.
32663557	12	43	theme	specific	1342:1349	arg1	properties					1382:1391	These specific physicochemical and structural properties	1336:1391	These specific physicochemical and structural properties	1336:1391	These specific physicochemical and structural properties indicated the industrial potential of low-amylose yam starch.
32663557	8	44	theme	NSY	732:734	arg1	starch					736:741	NSY starch	732:741	NSY starch	732:741	NSY starch had higher gelatinization temperature (71.5 °C), enthalpy (14.14 J/g), peak viscosity (8590 cP) and swelling power (12.0%) than TSY and HSY.
32663557	12	45	theme	yam	1443:1445	arg1	starch					1447:1452	low-amylose yam starch	1431:1452	low-amylose yam starch	1431:1452	These specific physicochemical and structural properties indicated the industrial potential of low-amylose yam starch.
32663557	8	46	contain	had	743:745	arg2	71.5 °C					782:788	71.5 °C	782:788	71.5 °C	782:788	NSY starch had higher gelatinization temperature (71.5 °C), enthalpy (14.14 J/g), peak viscosity (8590 cP) and swelling power (12.0%) than TSY and HSY.
32663557	8	46	contain	had	743:745	arg2	enthalpy					792:799	enthalpy	792:799	enthalpy (14.14 J/g)	792:811	NSY starch had higher gelatinization temperature (71.5 °C), enthalpy (14.14 J/g), peak viscosity (8590 cP) and swelling power (12.0%) than TSY and HSY.
32663557	8	46	contain	had	743:745	arg2	viscosity					819:827	peak viscosity	814:827	peak viscosity (8590 cP)	814:837	NSY starch had higher gelatinization temperature (71.5 °C), enthalpy (14.14 J/g), peak viscosity (8590 cP) and swelling power (12.0%) than TSY and HSY.
32663557	8	46	contain	had	743:745	arg1	starch					736:741	NSY starch	732:741	NSY starch	732:741	NSY starch had higher gelatinization temperature (71.5 °C), enthalpy (14.14 J/g), peak viscosity (8590 cP) and swelling power (12.0%) than TSY and HSY.
32663557	8	46	contain	had	743:745	arg2	14.14 J/g					802:810	14.14 J/g	802:810	14.14 J/g	802:810	NSY starch had higher gelatinization temperature (71.5 °C), enthalpy (14.14 J/g), peak viscosity (8590 cP) and swelling power (12.0%) than TSY and HSY.
32663557	8	46	contain	had	743:745	arg2	temperature					769:779	higher gelatinization temperature	747:779	higher gelatinization temperature (71.5 °C)	747:789	NSY starch had higher gelatinization temperature (71.5 °C), enthalpy (14.14 J/g), peak viscosity (8590 cP) and swelling power (12.0%) than TSY and HSY.
32663557	8	46	contain	had	743:745	arg2	8590 cP					830:836	8590 cP	830:836	8590 cP	830:836	NSY starch had higher gelatinization temperature (71.5 °C), enthalpy (14.14 J/g), peak viscosity (8590 cP) and swelling power (12.0%) than TSY and HSY.
32663557	8	46	contain	had	743:745	arg2	%					863:863	12.0%	859:863	12.0%	859:863	NSY starch had higher gelatinization temperature (71.5 °C), enthalpy (14.14 J/g), peak viscosity (8590 cP) and swelling power (12.0%) than TSY and HSY.
32663557	8	46	contain	had	743:745	arg2	power					852:856	swelling power	843:856	swelling power (12.0%)	843:864	NSY starch had higher gelatinization temperature (71.5 °C), enthalpy (14.14 J/g), peak viscosity (8590 cP) and swelling power (12.0%) than TSY and HSY.
32663557	9	47	dep	infrared	930:937	arg1	FTIR					940:943	FTIR	940:943	FTIR	940:943	X-ray diffraction (XRD) and Fourier transform infrared (FTIR) tests indicated that Chinese yam starches had CB-type crystalline structure with crystallinity ranging from 21.91% to 27.08% and a short-range ordered structure.
32663557	9	48	theme	X-ray	884:888	arg1	diffraction					890:900	X-ray diffraction	884:900	X-ray diffraction (XRD)	884:906	X-ray diffraction (XRD) and Fourier transform infrared (FTIR) tests indicated that Chinese yam starches had CB-type crystalline structure with crystallinity ranging from 21.91% to 27.08% and a short-range ordered structure.
32663557	9	48	theme	X-ray	884:888	arg1	XRD					903:905	XRD	903:905	XRD	903:905	X-ray diffraction (XRD) and Fourier transform infrared (FTIR) tests indicated that Chinese yam starches had CB-type crystalline structure with crystallinity ranging from 21.91% to 27.08% and a short-range ordered structure.
32663557	4	49	from	cultivars	363:371	arg1	properties					325:334	thermal properties	317:334	thermal properties	317:334	The composition, physicochemical properties, morphology, and thermal properties of the starches from these cultivars were investigated in this study.
32663557	4	49	from	cultivars	363:371	arg1	starches					343:350	the starches	339:350	the starches from these cultivars	339:371	The composition, physicochemical properties, morphology, and thermal properties of the starches from these cultivars were investigated in this study.
32663557	4	49	from	cultivars	363:371	arg1	morphology					301:310	morphology	301:310	morphology	301:310	The composition, physicochemical properties, morphology, and thermal properties of the starches from these cultivars were investigated in this study.
32663557	4	49	from	cultivars	363:371	arg1	composition					260:270	The composition	256:270	The composition	256:270	The composition, physicochemical properties, morphology, and thermal properties of the starches from these cultivars were investigated in this study.
32663557	4	49	from	cultivars	363:371	arg1	properties					289:298	physicochemical properties	273:298	physicochemical properties	273:298	The composition, physicochemical properties, morphology, and thermal properties of the starches from these cultivars were investigated in this study.
32663557	7	50	theme	Weight-average	571:584	arg1	weights					596:602	Weight-average molecular weights	571:602	Weight-average molecular weights (Mw)	571:607	Weight-average molecular weights (Mw) and number-average molecular weights (Mn) ranged from 1.29 to 1.84 × 105 g/mol and 5.93 to 8.36 × 104 g/mol, respectively.
32663557	7	50	theme	Weight-average	571:584	arg1	Mw					605:606	Mw	605:606	Mw	605:606	Weight-average molecular weights (Mw) and number-average molecular weights (Mn) ranged from 1.29 to 1.84 × 105 g/mol and 5.93 to 8.36 × 104 g/mol, respectively.
32663557	9	51	theme	Chinese	967:973	arg1	starches					979:986	Chinese yam starches	967:986	Chinese yam starches	967:986	X-ray diffraction (XRD) and Fourier transform infrared (FTIR) tests indicated that Chinese yam starches had CB-type crystalline structure with crystallinity ranging from 21.91% to 27.08% and a short-range ordered structure.
32663557	9	52	theme	CB-type	992:998	arg1	structure					1012:1020	CB-type crystalline structure	992:1020	CB-type crystalline structure	992:1020	X-ray diffraction (XRD) and Fourier transform infrared (FTIR) tests indicated that Chinese yam starches had CB-type crystalline structure with crystallinity ranging from 21.91% to 27.08% and a short-range ordered structure.
32663557	11	53	theme	peak	1301:1304	arg1	viscosity					1306:1314	peak viscosity	1301:1314	peak viscosity	1301:1314	The low-amylose NSY starch was found to have high gelatinization temperature, enthalpy, peak viscosity and swelling power.
32663557	0	54	theme	Dioscorea	70:78	arg1	Thunb					89:93	Dioscorea opposita Thunb.	70:94	Dioscorea opposita Thunb.	70:94	Physicochemical and structural properties of low-amylose Chinese yam (Dioscorea opposita Thunb.)
32663557	0	54	theme	Dioscorea	70:78	arg1	yam					65:67	low-amylose Chinese yam	45:67	low-amylose Chinese yam (Dioscorea opposita Thunb.)	45:95	Physicochemical and structural properties of low-amylose Chinese yam (Dioscorea opposita Thunb.)
32663557	7	55	theme	molecular	586:594	arg1	weights					596:602	Weight-average molecular weights	571:602	Weight-average molecular weights (Mw)	571:607	Weight-average molecular weights (Mw) and number-average molecular weights (Mn) ranged from 1.29 to 1.84 × 105 g/mol and 5.93 to 8.36 × 104 g/mol, respectively.
32663557	7	55	theme	molecular	586:594	arg1	Mw					605:606	Mw	605:606	Mw	605:606	Weight-average molecular weights (Mw) and number-average molecular weights (Mn) ranged from 1.29 to 1.84 × 105 g/mol and 5.93 to 8.36 × 104 g/mol, respectively.
32663557	4	56	from	composition	260:270	arg1	cultivars					363:371	these cultivars	357:371	these cultivars	357:371	The composition, physicochemical properties, morphology, and thermal properties of the starches from these cultivars were investigated in this study.
32663557	5	57	theme	amylose	443:449	arg1	content					451:457	amylose content	443:457	amylose content	443:457	NSY starch (17.0%) was much lower in amylose content than other cultivars (33.4-34.5%).
32663557	5	58	theme	NSY	406:408	arg1	starch					410:415	NSY starch	406:415	NSY starch (17.0%)	406:423	NSY starch (17.0%) was much lower in amylose content than other cultivars (33.4-34.5%).
32663557	5	58	theme	NSY	406:408	arg1	%					422:422	17.0%	418:422	17.0%	418:422	NSY starch (17.0%) was much lower in amylose content than other cultivars (33.4-34.5%).
32663557	11	59	theme	low-amylose	1217:1227	arg1	starch					1233:1238	The low-amylose NSY starch	1213:1238	The low-amylose NSY starch	1213:1238	The low-amylose NSY starch was found to have high gelatinization temperature, enthalpy, peak viscosity and swelling power.
32663557	2	60	theme	yam	201:203	arg1	NSY					119:121	NSY	119:121	NSY	119:121	Nuoshanyao (NSY), Tiegunshanyao (TSY) and Huaishanyao (HSY) are the main cultivars of Chinese yam (Dioscorea opposita Thunb.)
32663557	2	60	theme	yam	201:203	arg1	cultivars					180:188	the main cultivars	171:188	the main cultivars of Chinese yam	171:203	Nuoshanyao (NSY), Tiegunshanyao (TSY) and Huaishanyao (HSY) are the main cultivars of Chinese yam (Dioscorea opposita Thunb.)
32663557	2	60	theme	yam	201:203	arg1	HSY					162:164	HSY	162:164	HSY	162:164	Nuoshanyao (NSY), Tiegunshanyao (TSY) and Huaishanyao (HSY) are the main cultivars of Chinese yam (Dioscorea opposita Thunb.)
32663557	2	60	theme	yam	201:203	arg1	TSY					140:142	TSY	140:142	TSY	140:142	Nuoshanyao (NSY), Tiegunshanyao (TSY) and Huaishanyao (HSY) are the main cultivars of Chinese yam (Dioscorea opposita Thunb.)
32663557	4	61	theme	physicochemical	273:287	arg1	properties					289:298	physicochemical properties	273:298	physicochemical properties	273:298	The composition, physicochemical properties, morphology, and thermal properties of the starches from these cultivars were investigated in this study.
32663557	8	62	theme	gelatinization	754:767	arg1	71.5 °C					782:788	71.5 °C	782:788	71.5 °C	782:788	NSY starch had higher gelatinization temperature (71.5 °C), enthalpy (14.14 J/g), peak viscosity (8590 cP) and swelling power (12.0%) than TSY and HSY.
32663557	8	62	theme	gelatinization	754:767	arg1	temperature					769:779	higher gelatinization temperature	747:779	higher gelatinization temperature (71.5 °C)	747:789	NSY starch had higher gelatinization temperature (71.5 °C), enthalpy (14.14 J/g), peak viscosity (8590 cP) and swelling power (12.0%) than TSY and HSY.
32663557	12	63	theme	industrial	1407:1416	arg1	potential					1418:1426	the industrial potential	1403:1426	the industrial potential of low-amylose yam starch	1403:1452	These specific physicochemical and structural properties indicated the industrial potential of low-amylose yam starch.
34242644	3	0	from	increase	714:721	arg1	area					738:741	the surface area	726:741	the surface area	726:741	The composites exhibited a nanostructured scaffold with a particle size of 10 nm and experienced an unprecedented increase in the surface area by about 300% and improved d-spacing sheets by 17%.
34242644	1	1	theme	compounds	345:353	arg1	purification					319:330	purification	319:330	purification	319:330	This work provides a new perception toward the application of the graphenic-biopolymeric composites as a solid-bed for separation and purification of bioactive compounds.
34242644	1	1	theme	compounds	345:353	arg1	separation					304:313	separation	304:313	separation	304:313	This work provides a new perception toward the application of the graphenic-biopolymeric composites as a solid-bed for separation and purification of bioactive compounds.
34242644	7	2	theme	crocin	1191:1196	arg1	process					1277:1283	an endothermic, spontaneous and physisorption process	1231:1283	an endothermic, spontaneous and physisorption process	1231:1283	The thermodynamic studies indicated that the crocin adsorption on nanocomposites was an endothermic, spontaneous and physisorption process.
34242644	7	2	theme	crocin	1191:1196	arg1	adsorption					1198:1207	the crocin adsorption	1187:1207	the crocin adsorption on nanocomposites	1187:1225	The thermodynamic studies indicated that the crocin adsorption on nanocomposites was an endothermic, spontaneous and physisorption process.
34242644	5	3	theme	30 min	1033:1038	arg1	time					1025:1028	an equilibrium time	1010:1028	an equilibrium time of 30 min	1010:1038	Under these conditions, the nanocomposites separated 99.1% of crocin in an equilibrium time of 30 min.
34242644	6	4	theme	adsorption	1045:1054	arg1	data					1056:1059	The adsorption data	1041:1059	The adsorption data	1041:1059	The adsorption data were best represented by Freundlich isotherm and pseudo-second-order kinetic models.
34242644	0	5	theme	saffron	168:174	arg1	extract					176:182	saffron extract	168:182	saffron extract	168:182	Surface-decorated graphene oxide sheets with nanoparticles of chitosan-Arabic gum for the separation of bioactive compounds: A case study for adsorption of crocin from saffron extract.
34242644	2	6	theme	oxide	365:369	arg1	nanocomposites					371:384	Graphene oxide nanocomposites	356:384	Graphene oxide nanocomposites	356:384	Graphene oxide nanocomposites with functionalized sheets by soluble and insoluble nanocomplexes of chitosan and Arabic gum, were successfully synthesized and employed for the adsorption and purification of crocin, a nutraceutical from saffron.
34242644	2	7	from	saffron	591:597	arg1	nutraceutical					572:584	a nutraceutical	570:584	a nutraceutical from saffron	570:597	Graphene oxide nanocomposites with functionalized sheets by soluble and insoluble nanocomplexes of chitosan and Arabic gum, were successfully synthesized and employed for the adsorption and purification of crocin, a nutraceutical from saffron.
34242644	2	7	from	saffron	591:597	arg1	crocin					562:567	crocin	562:567	crocin	562:567	Graphene oxide nanocomposites with functionalized sheets by soluble and insoluble nanocomplexes of chitosan and Arabic gum, were successfully synthesized and employed for the adsorption and purification of crocin, a nutraceutical from saffron.
34242644	0	8	theme	bioactive	104:112	arg1	compounds					114:122	bioactive compounds	104:122	bioactive compounds	104:122	Surface-decorated graphene oxide sheets with nanoparticles of chitosan-Arabic gum for the separation of bioactive compounds: A case study for adsorption of crocin from saffron extract.
34242644	3	9	theme	surface	730:736	arg1	area					738:741	the surface area	726:741	the surface area	726:741	The composites exhibited a nanostructured scaffold with a particle size of 10 nm and experienced an unprecedented increase in the surface area by about 300% and improved d-spacing sheets by 17%.
34242644	1	10	theme	graphenic-biopolymeric	251:272	arg1	composites					274:283	the graphenic-biopolymeric composites	247:283	the graphenic-biopolymeric composites	247:283	This work provides a new perception toward the application of the graphenic-biopolymeric composites as a solid-bed for separation and purification of bioactive compounds.
34242644	8	11	theme	similar	1446:1452	arg1	purity					1439:1444	a purity	1437:1444	a purity similar to the standard sample	1437:1475	The high-performance liquid chromatography (HPLC) analysis revealed that produced nanocomposites adsorbed crocin efficiently from saffron extract with a purity similar to the standard sample.
34242644	5	12	theme	equilibrium	1013:1023	arg1	time					1025:1028	an equilibrium time	1010:1028	an equilibrium time of 30 min	1010:1038	Under these conditions, the nanocomposites separated 99.1% of crocin in an equilibrium time of 30 min.
34242644	1	13	theme	composites	274:283	arg1	application					232:242	the application	228:242	the application of the graphenic-biopolymeric composites	228:283	This work provides a new perception toward the application of the graphenic-biopolymeric composites as a solid-bed for separation and purification of bioactive compounds.
34242644	7	14	theme	physisorption	1263:1275	arg1	process					1277:1283	an endothermic, spontaneous and physisorption process	1231:1283	an endothermic, spontaneous and physisorption process	1231:1283	The thermodynamic studies indicated that the crocin adsorption on nanocomposites was an endothermic, spontaneous and physisorption process.
34242644	7	14	theme	physisorption	1263:1275	arg1	adsorption					1198:1207	the crocin adsorption	1187:1207	the crocin adsorption on nanocomposites	1187:1225	The thermodynamic studies indicated that the crocin adsorption on nanocomposites was an endothermic, spontaneous and physisorption process.
34242644	8	15	theme	standard	1461:1468	arg1	sample					1470:1475	the standard sample	1457:1475	the standard sample	1457:1475	The high-performance liquid chromatography (HPLC) analysis revealed that produced nanocomposites adsorbed crocin efficiently from saffron extract with a purity similar to the standard sample.
34242644	9	16	theme	hydrogen	1584:1591	arg1	bonding					1593:1599	hydrogen bonding	1584:1599	hydrogen bonding	1584:1599	The possible interaction mechanisms between crocin and nanocomposites were electrostatic interactions and hydrogen bonding.
34242644	0	17	theme	compounds	114:122	arg1	separation					90:99	the separation	86:99	the separation of bioactive compounds	86:122	Surface-decorated graphene oxide sheets with nanoparticles of chitosan-Arabic gum for the separation of bioactive compounds: A case study for adsorption of crocin from saffron extract.
34242644	0	18	theme	Surface-decorated	0:16	arg1	sheets					33:38	Surface-decorated graphene oxide sheets	0:38	Surface-decorated graphene oxide sheets with nanoparticles of chitosan-Arabic gum for the separation of bioactive compounds: A case study for adsorption of crocin from saffron extract.	0:183	Surface-decorated graphene oxide sheets with nanoparticles of chitosan-Arabic gum for the separation of bioactive compounds: A case study for adsorption of crocin from saffron extract.
34242644	2	19	theme	soluble	416:422	arg1	nanocomplexes					438:450	soluble and insoluble nanocomplexes	416:450	soluble and insoluble nanocomplexes of chitosan and Arabic gum	416:477	Graphene oxide nanocomposites with functionalized sheets by soluble and insoluble nanocomplexes of chitosan and Arabic gum, were successfully synthesized and employed for the adsorption and purification of crocin, a nutraceutical from saffron.
34242644	0	20	theme	graphene	18:25	arg1	sheets					33:38	Surface-decorated graphene oxide sheets	0:38	Surface-decorated graphene oxide sheets with nanoparticles of chitosan-Arabic gum for the separation of bioactive compounds: A case study for adsorption of crocin from saffron extract.	0:183	Surface-decorated graphene oxide sheets with nanoparticles of chitosan-Arabic gum for the separation of bioactive compounds: A case study for adsorption of crocin from saffron extract.
34242644	4	21	theme	initial	891:897	arg1	rate = 300 rpm					875:888	rate = 300 rpm	875:888	rate = 300 rpm	875:888	The optimum conditions for crocin separation were temperature = 318 K, stirring rate = 300 rpm, initial concentration = 100 mg L-1 and pH = 6.
34242644	4	21	theme	initial	891:897	arg1	concentration = 100 mg L-1					899:924	initial concentration = 100 mg L-1	891:924	initial concentration = 100 mg L-1	891:924	The optimum conditions for crocin separation were temperature = 318 K, stirring rate = 300 rpm, initial concentration = 100 mg L-1 and pH = 6.
34242644	6	22	theme	kinetic	1130:1136	arg1	models					1138:1143	pseudo-second-order kinetic models	1110:1143	pseudo-second-order kinetic models	1110:1143	The adsorption data were best represented by Freundlich isotherm and pseudo-second-order kinetic models.
34242644	2	23	theme	gum	475:477	arg1	nanocomplexes					438:450	soluble and insoluble nanocomplexes	416:450	soluble and insoluble nanocomplexes of chitosan and Arabic gum	416:477	Graphene oxide nanocomposites with functionalized sheets by soluble and insoluble nanocomplexes of chitosan and Arabic gum, were successfully synthesized and employed for the adsorption and purification of crocin, a nutraceutical from saffron.
34242644	3	24	theme	unprecedented	700:712	arg1	increase					714:721	an unprecedented increase	697:721	an unprecedented increase in the surface area	697:741	The composites exhibited a nanostructured scaffold with a particle size of 10 nm and experienced an unprecedented increase in the surface area by about 300% and improved d-spacing sheets by 17%.
34242644	6	25	theme	pseudo-second-order	1110:1128	arg1	models					1138:1143	pseudo-second-order kinetic models	1110:1143	pseudo-second-order kinetic models	1110:1143	The adsorption data were best represented by Freundlich isotherm and pseudo-second-order kinetic models.
34242644	2	26	theme	Arabic	468:473	arg1	gum					475:477	Arabic gum	468:477	Arabic gum	468:477	Graphene oxide nanocomposites with functionalized sheets by soluble and insoluble nanocomplexes of chitosan and Arabic gum, were successfully synthesized and employed for the adsorption and purification of crocin, a nutraceutical from saffron.
34242644	0	27	theme	case	127:130	arg1	study					132:136	A case study	125:136	Surface-decorated graphene oxide sheets with nanoparticles of chitosan-Arabic gum for the separation of bioactive compounds: A case study for adsorption of crocin from saffron extract.	0:183	Surface-decorated graphene oxide sheets with nanoparticles of chitosan-Arabic gum for the separation of bioactive compounds: A case study for adsorption of crocin from saffron extract.
34242644	5	28	theme	crocin	1000:1005	arg1	crocin					1000:1005	crocin	1000:1005	crocin	1000:1005	Under these conditions, the nanocomposites separated 99.1% of crocin in an equilibrium time of 30 min.
34242644	5	28	theme	crocin	1000:1005	arg1	%					995:995	99.1%	991:995	99.1% of crocin	991:1005	Under these conditions, the nanocomposites separated 99.1% of crocin in an equilibrium time of 30 min.
34242644	2	29	theme	chitosan	455:462	arg1	nanocomplexes					438:450	soluble and insoluble nanocomplexes	416:450	soluble and insoluble nanocomplexes of chitosan and Arabic gum	416:477	Graphene oxide nanocomposites with functionalized sheets by soluble and insoluble nanocomplexes of chitosan and Arabic gum, were successfully synthesized and employed for the adsorption and purification of crocin, a nutraceutical from saffron.
34242644	0	30	theme	oxide	27:31	arg1	sheets					33:38	Surface-decorated graphene oxide sheets	0:38	Surface-decorated graphene oxide sheets with nanoparticles of chitosan-Arabic gum for the separation of bioactive compounds: A case study for adsorption of crocin from saffron extract.	0:183	Surface-decorated graphene oxide sheets with nanoparticles of chitosan-Arabic gum for the separation of bioactive compounds: A case study for adsorption of crocin from saffron extract.
34242644	0	31	from	extract	176:182	arg1	adsorption					142:151	adsorption	142:151	adsorption of crocin from saffron extract	142:182	Surface-decorated graphene oxide sheets with nanoparticles of chitosan-Arabic gum for the separation of bioactive compounds: A case study for adsorption of crocin from saffron extract.
34242644	9	32	theme	electrostatic	1553:1565	arg1	interactions					1567:1578	electrostatic interactions	1553:1578	electrostatic interactions	1553:1578	The possible interaction mechanisms between crocin and nanocomposites were electrostatic interactions and hydrogen bonding.
34242644	9	32	theme	electrostatic	1553:1565	arg1	mechanisms					1503:1512	The possible interaction mechanisms	1478:1512	The possible interaction mechanisms between crocin and nanocomposites	1478:1546	The possible interaction mechanisms between crocin and nanocomposites were electrostatic interactions and hydrogen bonding.
34242644	8	33	theme	liquid	1307:1312	arg1	HPLC					1330:1333	HPLC	1330:1333	HPLC	1330:1333	The high-performance liquid chromatography (HPLC) analysis revealed that produced nanocomposites adsorbed crocin efficiently from saffron extract with a purity similar to the standard sample.
34242644	8	33	theme	liquid	1307:1312	arg1	chromatography					1314:1327	The high-performance liquid chromatography	1286:1327	The high-performance liquid chromatography (HPLC) analysis	1286:1343	The high-performance liquid chromatography (HPLC) analysis revealed that produced nanocomposites adsorbed crocin efficiently from saffron extract with a purity similar to the standard sample.
34242644	3	34	theme	d-spacing	770:778	arg1	sheets					780:785	d-spacing sheets	770:785	d-spacing sheets	770:785	The composites exhibited a nanostructured scaffold with a particle size of 10 nm and experienced an unprecedented increase in the surface area by about 300% and improved d-spacing sheets by 17%.
34242644	8	35	theme	adsorbed	1383:1390	arg1	crocin					1392:1397	nanocomposites adsorbed crocin	1368:1397	nanocomposites adsorbed crocin	1368:1397	The high-performance liquid chromatography (HPLC) analysis revealed that produced nanocomposites adsorbed crocin efficiently from saffron extract with a purity similar to the standard sample.
34242644	3	36	theme	nanostructured	627:640	arg1	scaffold					642:649	a nanostructured scaffold	625:649	a nanostructured scaffold	625:649	The composites exhibited a nanostructured scaffold with a particle size of 10 nm and experienced an unprecedented increase in the surface area by about 300% and improved d-spacing sheets by 17%.
34242644	6	37	theme	Freundlich	1086:1095	arg1	isotherm					1097:1104	Freundlich isotherm	1086:1104	Freundlich isotherm	1086:1104	The adsorption data were best represented by Freundlich isotherm and pseudo-second-order kinetic models.
34242644	8	38	theme	high-performance	1290:1305	arg1	HPLC					1330:1333	HPLC	1330:1333	HPLC	1330:1333	The high-performance liquid chromatography (HPLC) analysis revealed that produced nanocomposites adsorbed crocin efficiently from saffron extract with a purity similar to the standard sample.
34242644	8	38	theme	high-performance	1290:1305	arg1	chromatography					1314:1327	The high-performance liquid chromatography	1286:1327	The high-performance liquid chromatography (HPLC) analysis	1286:1343	The high-performance liquid chromatography (HPLC) analysis revealed that produced nanocomposites adsorbed crocin efficiently from saffron extract with a purity similar to the standard sample.
34242644	9	39	theme	possible	1482:1489	arg1	interactions					1567:1578	electrostatic interactions	1553:1578	electrostatic interactions	1553:1578	The possible interaction mechanisms between crocin and nanocomposites were electrostatic interactions and hydrogen bonding.
34242644	9	39	theme	possible	1482:1489	arg1	mechanisms					1503:1512	The possible interaction mechanisms	1478:1512	The possible interaction mechanisms between crocin and nanocomposites	1478:1546	The possible interaction mechanisms between crocin and nanocomposites were electrostatic interactions and hydrogen bonding.
34242644	7	40	theme	endothermic	1234:1244	arg1	process					1277:1283	an endothermic, spontaneous and physisorption process	1231:1283	an endothermic, spontaneous and physisorption process	1231:1283	The thermodynamic studies indicated that the crocin adsorption on nanocomposites was an endothermic, spontaneous and physisorption process.
34242644	7	40	theme	endothermic	1234:1244	arg1	adsorption					1198:1207	the crocin adsorption	1187:1207	the crocin adsorption on nanocomposites	1187:1225	The thermodynamic studies indicated that the crocin adsorption on nanocomposites was an endothermic, spontaneous and physisorption process.
34242644	0	41	with	sheets	33:38	arg1	nanoparticles					45:57	nanoparticles	45:57	nanoparticles of chitosan-Arabic gum	45:80	Surface-decorated graphene oxide sheets with nanoparticles of chitosan-Arabic gum for the separation of bioactive compounds: A case study for adsorption of crocin from saffron extract.
34242644	1	42	theme	new	206:208	arg1	solid-bed					290:298	a solid-bed	288:298	a solid-bed for separation and purification of bioactive compounds	288:353	This work provides a new perception toward the application of the graphenic-biopolymeric composites as a solid-bed for separation and purification of bioactive compounds.
34242644	1	42	theme	new	206:208	arg1	perception					210:219	a new perception	204:219	a new perception toward the application of the graphenic-biopolymeric composites	204:283	This work provides a new perception toward the application of the graphenic-biopolymeric composites as a solid-bed for separation and purification of bioactive compounds.
34242644	0	43	theme	chitosan-Arabic	62:76	arg1	gum					78:80	chitosan-Arabic gum	62:80	chitosan-Arabic gum	62:80	Surface-decorated graphene oxide sheets with nanoparticles of chitosan-Arabic gum for the separation of bioactive compounds: A case study for adsorption of crocin from saffron extract.
34242644	2	44	theme	crocin	562:567	arg1	purification					546:557	purification	546:557	purification	546:557	Graphene oxide nanocomposites with functionalized sheets by soluble and insoluble nanocomplexes of chitosan and Arabic gum, were successfully synthesized and employed for the adsorption and purification of crocin, a nutraceutical from saffron.
34242644	2	44	theme	crocin	562:567	arg1	adsorption					531:540	adsorption	531:540	adsorption	531:540	Graphene oxide nanocomposites with functionalized sheets by soluble and insoluble nanocomplexes of chitosan and Arabic gum, were successfully synthesized and employed for the adsorption and purification of crocin, a nutraceutical from saffron.
34242644	3	45	theme	10 nm	675:679	arg1	size					667:670	a particle size	656:670	a particle size of 10 nm	656:679	The composites exhibited a nanostructured scaffold with a particle size of 10 nm and experienced an unprecedented increase in the surface area by about 300% and improved d-spacing sheets by 17%.
34242644	2	46	theme	Graphene	356:363	arg1	nanocomposites					371:384	Graphene oxide nanocomposites	356:384	Graphene oxide nanocomposites	356:384	Graphene oxide nanocomposites with functionalized sheets by soluble and insoluble nanocomplexes of chitosan and Arabic gum, were successfully synthesized and employed for the adsorption and purification of crocin, a nutraceutical from saffron.
34242644	8	47	theme	chromatography	1314:1327	arg1	analysis					1336:1343	The high-performance liquid chromatography (HPLC) analysis	1286:1343	The high-performance liquid chromatography (HPLC) analysis	1286:1343	The high-performance liquid chromatography (HPLC) analysis revealed that produced nanocomposites adsorbed crocin efficiently from saffron extract with a purity similar to the standard sample.
34242644	2	48	with	nanocomposites	371:384	arg1	sheets					406:411	functionalized sheets	391:411	functionalized sheets	391:411	Graphene oxide nanocomposites with functionalized sheets by soluble and insoluble nanocomplexes of chitosan and Arabic gum, were successfully synthesized and employed for the adsorption and purification of crocin, a nutraceutical from saffron.
34242644	7	49	from	adsorption	1198:1207	arg1	nanocomposites					1212:1225	nanocomposites	1212:1225	nanocomposites	1212:1225	The thermodynamic studies indicated that the crocin adsorption on nanocomposites was an endothermic, spontaneous and physisorption process.
34242644	2	50	dep	adsorption	531:540	arg1	the					527:529	the	527:529	the	527:529	Graphene oxide nanocomposites with functionalized sheets by soluble and insoluble nanocomplexes of chitosan and Arabic gum, were successfully synthesized and employed for the adsorption and purification of crocin, a nutraceutical from saffron.
34242644	3	51	theme	particle	658:665	arg1	size					667:670	a particle size	656:670	a particle size of 10 nm	656:679	The composites exhibited a nanostructured scaffold with a particle size of 10 nm and experienced an unprecedented increase in the surface area by about 300% and improved d-spacing sheets by 17%.
34242644	0	52	dep	sheets	33:38	arg1	study					132:136	A case study	125:136	Surface-decorated graphene oxide sheets with nanoparticles of chitosan-Arabic gum for the separation of bioactive compounds: A case study for adsorption of crocin from saffron extract.	0:183	Surface-decorated graphene oxide sheets with nanoparticles of chitosan-Arabic gum for the separation of bioactive compounds: A case study for adsorption of crocin from saffron extract.
34242644	8	53	theme	saffron	1416:1422	arg1	extract					1424:1430	saffron extract	1416:1430	saffron extract	1416:1430	The high-performance liquid chromatography (HPLC) analysis revealed that produced nanocomposites adsorbed crocin efficiently from saffron extract with a purity similar to the standard sample.
34242644	9	54	theme	interaction	1491:1501	arg1	interactions					1567:1578	electrostatic interactions	1553:1578	electrostatic interactions	1553:1578	The possible interaction mechanisms between crocin and nanocomposites were electrostatic interactions and hydrogen bonding.
34242644	9	54	theme	interaction	1491:1501	arg1	mechanisms					1503:1512	The possible interaction mechanisms	1478:1512	The possible interaction mechanisms between crocin and nanocomposites	1478:1546	The possible interaction mechanisms between crocin and nanocomposites were electrostatic interactions and hydrogen bonding.
34242644	8	55	theme	nanocomposites	1368:1381	arg1	crocin					1392:1397	nanocomposites adsorbed crocin	1368:1397	nanocomposites adsorbed crocin	1368:1397	The high-performance liquid chromatography (HPLC) analysis revealed that produced nanocomposites adsorbed crocin efficiently from saffron extract with a purity similar to the standard sample.
34242644	0	56	theme	crocin	156:161	arg1	adsorption					142:151	adsorption	142:151	adsorption of crocin from saffron extract	142:182	Surface-decorated graphene oxide sheets with nanoparticles of chitosan-Arabic gum for the separation of bioactive compounds: A case study for adsorption of crocin from saffron extract.
34242644	4	57	theme	optimum	799:805	arg1	conditions					807:816	The optimum conditions	795:816	The optimum conditions for crocin separation	795:838	The optimum conditions for crocin separation were temperature = 318 K, stirring rate = 300 rpm, initial concentration = 100 mg L-1 and pH = 6.
34242644	7	58	theme	thermodynamic	1150:1162	arg1	studies					1164:1170	The thermodynamic studies	1146:1170	The thermodynamic studies	1146:1170	The thermodynamic studies indicated that the crocin adsorption on nanocomposites was an endothermic, spontaneous and physisorption process.
34242644	2	59	theme	insoluble	428:436	arg1	nanocomplexes					438:450	soluble and insoluble nanocomplexes	416:450	soluble and insoluble nanocomplexes of chitosan and Arabic gum	416:477	Graphene oxide nanocomposites with functionalized sheets by soluble and insoluble nanocomplexes of chitosan and Arabic gum, were successfully synthesized and employed for the adsorption and purification of crocin, a nutraceutical from saffron.
34242644	4	60	theme	crocin	822:827	arg1	separation					829:838	crocin separation	822:838	crocin separation	822:838	The optimum conditions for crocin separation were temperature = 318 K, stirring rate = 300 rpm, initial concentration = 100 mg L-1 and pH = 6.
34242644	2	61	theme	functionalized	391:404	arg1	sheets					406:411	functionalized sheets	391:411	functionalized sheets	391:411	Graphene oxide nanocomposites with functionalized sheets by soluble and insoluble nanocomplexes of chitosan and Arabic gum, were successfully synthesized and employed for the adsorption and purification of crocin, a nutraceutical from saffron.
34242644	0	62	theme	gum	78:80	arg1	nanoparticles					45:57	nanoparticles	45:57	nanoparticles of chitosan-Arabic gum	45:80	Surface-decorated graphene oxide sheets with nanoparticles of chitosan-Arabic gum for the separation of bioactive compounds: A case study for adsorption of crocin from saffron extract.
34242644	1	63	theme	bioactive	335:343	arg1	compounds					345:353	bioactive compounds	335:353	bioactive compounds	335:353	This work provides a new perception toward the application of the graphenic-biopolymeric composites as a solid-bed for separation and purification of bioactive compounds.
34242644	7	64	theme	spontaneous	1247:1257	arg1	process					1277:1283	an endothermic, spontaneous and physisorption process	1231:1283	an endothermic, spontaneous and physisorption process	1231:1283	The thermodynamic studies indicated that the crocin adsorption on nanocomposites was an endothermic, spontaneous and physisorption process.
34242644	7	64	theme	spontaneous	1247:1257	arg1	adsorption					1198:1207	the crocin adsorption	1187:1207	the crocin adsorption on nanocomposites	1187:1225	The thermodynamic studies indicated that the crocin adsorption on nanocomposites was an endothermic, spontaneous and physisorption process.
32240741	9	0	theme	high	1071:1074	arg1	porosity					1076:1083	nanofibrous mat's high porosity	1053:1083	nanofibrous mat's high porosity (85-90%)	1053:1092	Besides, the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m2/day, suitable for wound breathability and the mats show high cytocompatibility to NIH 3T3 cell line in in vitro testing, proving to be effective for promoting wound healing.
32240741	9	0	theme	high	1071:1074	arg1	%					1091:1091	85-90%	1086:1091	85-90%	1086:1091	Besides, the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m2/day, suitable for wound breathability and the mats show high cytocompatibility to NIH 3T3 cell line in in vitro testing, proving to be effective for promoting wound healing.
32240741	9	1	theme	nanofibrous	1053:1063	arg1	porosity					1076:1083	nanofibrous mat's high porosity	1053:1083	nanofibrous mat's high porosity (85-90%)	1053:1092	Besides, the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m2/day, suitable for wound breathability and the mats show high cytocompatibility to NIH 3T3 cell line in in vitro testing, proving to be effective for promoting wound healing.
32240741	9	1	theme	nanofibrous	1053:1063	arg1	%					1091:1091	85-90%	1086:1091	85-90%	1086:1091	Besides, the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m2/day, suitable for wound breathability and the mats show high cytocompatibility to NIH 3T3 cell line in in vitro testing, proving to be effective for promoting wound healing.
32240741	0	2	theme	in	78:79	arg1	Fabrication					62:72	Fabrication	62:72	Fabrication	62:72	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	0	2	theme	in	78:79	arg1	mats					56:59	cellulose acetate nanofibrous mats	26:59	cellulose acetate nanofibrous mats	26:59	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	0	2	theme	in	78:79	arg1	dressing					119:126	a potential wound dressing	101:126	a potential wound dressing	101:126	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	0	2	theme	in	78:79	arg1	evaluation					87:96	in vitro evaluation	78:96	in vitro evaluation	78:96	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	9	3	dep	in	1234:1235	arg1	vitro					1237:1241	vitro	1237:1241	vitro	1237:1241	Besides, the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m2/day, suitable for wound breathability and the mats show high cytocompatibility to NIH 3T3 cell line in in vitro testing, proving to be effective for promoting wound healing.
32240741	4	4	from	inclusion	485:493	arg1	mats					536:539	the composite CA-MH nanofibrous mats	504:539	the composite CA-MH nanofibrous mats	504:539	The ATR-FTIR spectra confirm the inclusion of MH in the composite CA-MH nanofibrous mats.
32240741	7	5	theme	wound	806:810	arg1	surface					812:818	the wound surface	802:818	the wound surface	802:818	Inclusion of MH in the electrospun composite CA-MH nanofibrous mats shows high efficacy to prevent bacterial growth on the wound surface.
32240741	2	6	theme	mats	319:322	arg1	fabrication					241:251	the fabrication	237:251	the fabrication of cellulose acetate (CA)-Manuka honey (MH) composite nanofibrous mats as a biocompatible and antimicrobial wound dressing	237:374	Here we report the fabrication of cellulose acetate (CA)-Manuka honey (MH) composite nanofibrous mats as a biocompatible and antimicrobial wound dressing.
32240741	8	7	theme	CA	835:836	arg1	mats					848:851	The MH loaded CA nanofiber mats	821:851	The MH loaded CA nanofiber mats	821:851	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	7	8	theme	electrospun	706:716	arg1	mats					746:749	the electrospun composite CA-MH nanofibrous mats	702:749	the electrospun composite CA-MH nanofibrous mats	702:749	Inclusion of MH in the electrospun composite CA-MH nanofibrous mats shows high efficacy to prevent bacterial growth on the wound surface.
32240741	9	9	theme	NIH	1213:1215	arg1	3T3					1217:1219	NIH 3T3	1213:1219	NIH 3T3 cell line	1213:1229	Besides, the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m2/day, suitable for wound breathability and the mats show high cytocompatibility to NIH 3T3 cell line in in vitro testing, proving to be effective for promoting wound healing.
32240741	8	10	theme	nanofiber	838:846	arg1	mats					848:851	The MH loaded CA nanofiber mats	821:851	The MH loaded CA nanofiber mats	821:851	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	7	11	from	Inclusion	683:691	arg1	mats					746:749	the electrospun composite CA-MH nanofibrous mats	702:749	the electrospun composite CA-MH nanofibrous mats	702:749	Inclusion of MH in the electrospun composite CA-MH nanofibrous mats shows high efficacy to prevent bacterial growth on the wound surface.
32240741	8	12	theme	loaded	828:833	arg1	mats					848:851	The MH loaded CA nanofiber mats	821:851	The MH loaded CA nanofiber mats	821:851	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	7	13	theme	MH	696:697	arg1	Inclusion					683:691	Inclusion	683:691	Inclusion of MH in the electrospun composite CA-MH nanofibrous mats	683:749	Inclusion of MH in the electrospun composite CA-MH nanofibrous mats shows high efficacy to prevent bacterial growth on the wound surface.
32240741	9	14	theme	3T3	1217:1219	arg1	line					1226:1229	NIH 3T3 cell line	1213:1229	NIH 3T3 cell line	1213:1229	Besides, the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m2/day, suitable for wound breathability and the mats show high cytocompatibility to NIH 3T3 cell line in in vitro testing, proving to be effective for promoting wound healing.
32240741	0	15	theme	potential	103:111	arg1	Fabrication					62:72	Fabrication	62:72	Fabrication	62:72	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	0	15	theme	potential	103:111	arg1	mats					56:59	cellulose acetate nanofibrous mats	26:59	cellulose acetate nanofibrous mats	26:59	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	0	15	theme	potential	103:111	arg1	dressing					119:126	a potential wound dressing	101:126	a potential wound dressing	101:126	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	0	15	theme	potential	103:111	arg1	evaluation					87:96	in vitro evaluation	78:96	in vitro evaluation	78:96	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	8	16	from	factors	957:963	arg1	solution					1030:1037	the DPPH solution	1021:1037	the DPPH solution	1021:1037	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	8	16	from	factors	957:963	arg1	fiber					986:990	the fiber	982:990	the fiber	982:990	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	5	17	theme	mechanical	599:608	arg1	properties					610:619	acceptable mechanical properties	588:619	acceptable mechanical properties	588:619	The fibers were continuous and bead-free with acceptable mechanical properties.
32240741	5	18	theme	acceptable	588:597	arg1	properties					610:619	acceptable mechanical properties	588:619	acceptable mechanical properties	588:619	The fibers were continuous and bead-free with acceptable mechanical properties.
32240741	3	19	with	mats	380:383	arg1	quantities					400:409	different quantities	390:409	different quantities of MH	390:415	CA mats with different quantities of MH were developed by electrospinning.
32240741	3	20	theme	different	390:398	arg1	quantities					400:409	different quantities	390:409	different quantities of MH	390:415	CA mats with different quantities of MH were developed by electrospinning.
32240741	8	21	theme	MH	968:969	arg1	content					971:977	MH content	968:977	MH content in the fiber	968:990	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	8	22	theme	good	860:863	arg1	abilities					877:885	good antioxidant abilities	860:885	good antioxidant abilities	860:885	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	2	23	theme	wound	361:365	arg1	dressing					367:374	a biocompatible and antimicrobial wound dressing	327:374	a biocompatible and antimicrobial wound dressing	327:374	Here we report the fabrication of cellulose acetate (CA)-Manuka honey (MH) composite nanofibrous mats as a biocompatible and antimicrobial wound dressing.
32240741	8	24	from	time	1000:1003	arg1	solution					1030:1037	the DPPH solution	1021:1037	the DPPH solution	1021:1037	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	8	24	from	time	1000:1003	arg1	fiber					986:990	the fiber	982:990	the fiber	982:990	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	4	25	theme	ATR-FTIR	456:463	arg1	spectra					465:471	The ATR-FTIR spectra	452:471	The ATR-FTIR spectra	452:471	The ATR-FTIR spectra confirm the inclusion of MH in the composite CA-MH nanofibrous mats.
32240741	7	26	from	growth	792:797	arg1	surface					812:818	the wound surface	802:818	the wound surface	802:818	Inclusion of MH in the electrospun composite CA-MH nanofibrous mats shows high efficacy to prevent bacterial growth on the wound surface.
32240741	9	27	theme	in	1234:1235	arg1	testing					1243:1249	in vitro testing	1234:1249	in vitro testing	1234:1249	Besides, the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m2/day, suitable for wound breathability and the mats show high cytocompatibility to NIH 3T3 cell line in in vitro testing, proving to be effective for promoting wound healing.
32240741	6	28	from	increase	659:666	arg1	content					674:680	MH content	671:680	MH content	671:680	The fiber diameter increased with an increase in MH content.
32240741	9	29	theme	cell	1221:1224	arg1	line					1226:1229	NIH 3T3 cell line	1213:1229	NIH 3T3 cell line	1213:1229	Besides, the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m2/day, suitable for wound breathability and the mats show high cytocompatibility to NIH 3T3 cell line in in vitro testing, proving to be effective for promoting wound healing.
32240741	2	30	theme	antimicrobial	347:359	arg1	dressing					367:374	a biocompatible and antimicrobial wound dressing	327:374	a biocompatible and antimicrobial wound dressing	327:374	Here we report the fabrication of cellulose acetate (CA)-Manuka honey (MH) composite nanofibrous mats as a biocompatible and antimicrobial wound dressing.
32240741	0	31	theme	Manuka	0:5	arg1	honey					7:11	Manuka honey	0:11	Manuka honey	0:11	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	4	32	theme	nanofibrous	524:534	arg1	mats					536:539	the composite CA-MH nanofibrous mats	504:539	the composite CA-MH nanofibrous mats	504:539	The ATR-FTIR spectra confirm the inclusion of MH in the composite CA-MH nanofibrous mats.
32240741	8	33	theme	immersion	1008:1016	arg1	factors					957:963	the factors	953:963	the factors of MH content in the fiber	953:990	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	8	33	theme	immersion	1008:1016	arg1	time					1000:1003	the time	996:1003	the time of immersion in the DPPH solution	996:1037	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	9	34	theme	1950 g/m2/day	1121:1133	arg1	values					1103:1108	the nanofibrous mat's high porosity (85-90%) and WVTR values	1049:1108	the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m2/day, suitable for wound breathability	1049:1167	Besides, the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m2/day, suitable for wound breathability and the mats show high cytocompatibility to NIH 3T3 cell line in in vitro testing, proving to be effective for promoting wound healing.
32240741	9	34	theme	1950 g/m2/day	1121:1133	arg1	mats					1177:1180	the mats	1173:1180	the mats	1173:1180	Besides, the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m2/day, suitable for wound breathability and the mats show high cytocompatibility to NIH 3T3 cell line in in vitro testing, proving to be effective for promoting wound healing.
32240741	0	35	dep	in	78:79	arg1	vitro					81:85	vitro	81:85	vitro	81:85	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	6	36	theme	MH	671:672	arg1	content					674:680	MH content	671:680	MH content	671:680	The fiber diameter increased with an increase in MH content.
32240741	1	37	theme	wound	181:185	arg1	surface					187:193	the wound surface	177:193	the wound surface	177:193	Wound dressings are the primary barrier between the wound surface and the outer environment.
32240741	0	38	theme	cellulose	26:34	arg1	Fabrication					62:72	Fabrication	62:72	Fabrication	62:72	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	0	38	theme	cellulose	26:34	arg1	mats					56:59	cellulose acetate nanofibrous mats	26:59	cellulose acetate nanofibrous mats	26:59	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	0	38	theme	cellulose	26:34	arg1	dressing					119:126	a potential wound dressing	101:126	a potential wound dressing	101:126	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	0	38	theme	cellulose	26:34	arg1	evaluation					87:96	in vitro evaluation	78:96	in vitro evaluation	78:96	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	4	39	theme	CA-MH	518:522	arg1	mats					536:539	the composite CA-MH nanofibrous mats	504:539	the composite CA-MH nanofibrous mats	504:539	The ATR-FTIR spectra confirm the inclusion of MH in the composite CA-MH nanofibrous mats.
32240741	8	40	theme	DPPH	1025:1028	arg1	solution					1030:1037	the DPPH solution	1021:1037	the DPPH solution	1021:1037	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	8	41	theme	content	971:977	arg1	factors					957:963	the factors	953:963	the factors of MH content in the fiber	953:990	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	8	41	theme	content	971:977	arg1	time					1000:1003	the time	996:1003	the time of immersion in the DPPH solution	996:1037	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	9	42	theme	WVTR	1098:1101	arg1	values					1103:1108	the nanofibrous mat's high porosity (85-90%) and WVTR values	1049:1108	the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m2/day, suitable for wound breathability	1049:1167	Besides, the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m2/day, suitable for wound breathability and the mats show high cytocompatibility to NIH 3T3 cell line in in vitro testing, proving to be effective for promoting wound healing.
32240741	8	43	theme	antioxidant	865:875	arg1	abilities					877:885	good antioxidant abilities	860:885	good antioxidant abilities	860:885	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	8	44	from	fiber	986:990	arg1	factors					957:963	the factors	953:963	the factors of MH content in the fiber	953:990	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	8	44	from	fiber	986:990	arg1	time					1000:1003	the time	996:1003	the time of immersion in the DPPH solution	996:1037	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	0	45	theme	wound	113:117	arg1	Fabrication					62:72	Fabrication	62:72	Fabrication	62:72	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	0	45	theme	wound	113:117	arg1	mats					56:59	cellulose acetate nanofibrous mats	26:59	cellulose acetate nanofibrous mats	26:59	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	0	45	theme	wound	113:117	arg1	dressing					119:126	a potential wound dressing	101:126	a potential wound dressing	101:126	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	0	45	theme	wound	113:117	arg1	evaluation					87:96	in vitro evaluation	78:96	in vitro evaluation	78:96	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	1	46	theme	primary	153:159	arg1	barrier					161:167	the primary barrier	149:167	the primary barrier between the wound surface and the outer environment	149:219	Wound dressings are the primary barrier between the wound surface and the outer environment.
32240741	1	46	theme	primary	153:159	arg1	dressings					135:143	Wound dressings	129:143	Wound dressings	129:143	Wound dressings are the primary barrier between the wound surface and the outer environment.
32240741	0	47	theme	nanofibrous	44:54	arg1	Fabrication					62:72	Fabrication	62:72	Fabrication	62:72	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	0	47	theme	nanofibrous	44:54	arg1	mats					56:59	cellulose acetate nanofibrous mats	26:59	cellulose acetate nanofibrous mats	26:59	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	0	47	theme	nanofibrous	44:54	arg1	dressing					119:126	a potential wound dressing	101:126	a potential wound dressing	101:126	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	0	47	theme	nanofibrous	44:54	arg1	evaluation					87:96	in vitro evaluation	78:96	in vitro evaluation	78:96	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	7	48	theme	bacterial	782:790	arg1	growth					792:797	bacterial growth	782:797	bacterial growth on the wound surface	782:818	Inclusion of MH in the electrospun composite CA-MH nanofibrous mats shows high efficacy to prevent bacterial growth on the wound surface.
32240741	6	49	theme	fiber	626:630	arg1	diameter					632:639	The fiber diameter	622:639	The fiber diameter	622:639	The fiber diameter increased with an increase in MH content.
32240741	0	50	theme	acetate	36:42	arg1	Fabrication					62:72	Fabrication	62:72	Fabrication	62:72	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	0	50	theme	acetate	36:42	arg1	mats					56:59	cellulose acetate nanofibrous mats	26:59	cellulose acetate nanofibrous mats	26:59	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	0	50	theme	acetate	36:42	arg1	dressing					119:126	a potential wound dressing	101:126	a potential wound dressing	101:126	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	0	50	theme	acetate	36:42	arg1	evaluation					87:96	in vitro evaluation	78:96	in vitro evaluation	78:96	Manuka honey incorporated cellulose acetate nanofibrous mats: Fabrication and in vitro evaluation as a potential wound dressing.
32240741	9	51	theme	wound	1149:1153	arg1	breathability					1155:1167	wound breathability	1149:1167	wound breathability	1149:1167	Besides, the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m2/day, suitable for wound breathability and the mats show high cytocompatibility to NIH 3T3 cell line in in vitro testing, proving to be effective for promoting wound healing.
32240741	9	52	theme	wound	1290:1294	arg1	healing					1296:1302	wound healing	1290:1302	wound healing	1290:1302	Besides, the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m2/day, suitable for wound breathability and the mats show high cytocompatibility to NIH 3T3 cell line in in vitro testing, proving to be effective for promoting wound healing.
32240741	1	53	theme	outer	203:207	arg1	environment					209:219	the outer environment	199:219	the outer environment	199:219	Wound dressings are the primary barrier between the wound surface and the outer environment.
32240741	4	54	theme	MH	498:499	arg1	inclusion					485:493	the inclusion	481:493	the inclusion of MH in the composite CA-MH nanofibrous mats	481:539	The ATR-FTIR spectra confirm the inclusion of MH in the composite CA-MH nanofibrous mats.
32240741	5	55	with	continuous	558:567	arg1	properties					610:619	acceptable mechanical properties	588:619	acceptable mechanical properties	588:619	The fibers were continuous and bead-free with acceptable mechanical properties.
32240741	2	56	theme	nanofibrous	307:317	arg1	mats					319:322	cellulose acetate (CA)-Manuka honey (MH) composite nanofibrous mats	256:322	cellulose acetate (CA)-Manuka honey (MH) composite nanofibrous mats	256:322	Here we report the fabrication of cellulose acetate (CA)-Manuka honey (MH) composite nanofibrous mats as a biocompatible and antimicrobial wound dressing.
32240741	3	57	theme	CA	377:378	arg1	mats					380:383	CA mats	377:383	CA mats with different quantities of MH	377:415	CA mats with different quantities of MH were developed by electrospinning.
32240741	4	58	theme	composite	508:516	arg1	mats					536:539	the composite CA-MH nanofibrous mats	504:539	the composite CA-MH nanofibrous mats	504:539	The ATR-FTIR spectra confirm the inclusion of MH in the composite CA-MH nanofibrous mats.
32240741	8	59	theme	free	909:912	arg1	DPPH					929:932	free radicalize the DPPH	909:932	free radicalize the DPPH	909:932	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	2	60	theme	composite	297:305	arg1	mats					319:322	cellulose acetate (CA)-Manuka honey (MH) composite nanofibrous mats	256:322	cellulose acetate (CA)-Manuka honey (MH) composite nanofibrous mats	256:322	Here we report the fabrication of cellulose acetate (CA)-Manuka honey (MH) composite nanofibrous mats as a biocompatible and antimicrobial wound dressing.
32240741	7	61	theme	high	757:760	arg1	efficacy					762:769	high efficacy	757:769	high efficacy to prevent bacterial growth on the wound surface	757:818	Inclusion of MH in the electrospun composite CA-MH nanofibrous mats shows high efficacy to prevent bacterial growth on the wound surface.
32240741	9	62	theme	porosity	1076:1083	arg1	values					1103:1108	the nanofibrous mat's high porosity (85-90%) and WVTR values	1049:1108	the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m2/day, suitable for wound breathability	1049:1167	Besides, the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m2/day, suitable for wound breathability and the mats show high cytocompatibility to NIH 3T3 cell line in in vitro testing, proving to be effective for promoting wound healing.
32240741	7	63	theme	nanofibrous	734:744	arg1	mats					746:749	the electrospun composite CA-MH nanofibrous mats	702:749	the electrospun composite CA-MH nanofibrous mats	702:749	Inclusion of MH in the electrospun composite CA-MH nanofibrous mats shows high efficacy to prevent bacterial growth on the wound surface.
32240741	9	64	dep	1950 g/m2/day	1121:1133	arg1	to					1118:1119	to	1118:1119	to	1118:1119	Besides, the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m2/day, suitable for wound breathability and the mats show high cytocompatibility to NIH 3T3 cell line in in vitro testing, proving to be effective for promoting wound healing.
32240741	1	65	theme	Wound	129:133	arg1	barrier					161:167	the primary barrier	149:167	the primary barrier between the wound surface and the outer environment	149:219	Wound dressings are the primary barrier between the wound surface and the outer environment.
32240741	1	65	theme	Wound	129:133	arg1	dressings					135:143	Wound dressings	129:143	Wound dressings	129:143	Wound dressings are the primary barrier between the wound surface and the outer environment.
32240741	8	66	theme	radicalize	914:923	arg1	DPPH					929:932	free radicalize the DPPH	909:932	free radicalize the DPPH	909:932	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	2	67	theme	biocompatible	329:341	arg1	dressing					367:374	a biocompatible and antimicrobial wound dressing	327:374	a biocompatible and antimicrobial wound dressing	327:374	Here we report the fabrication of cellulose acetate (CA)-Manuka honey (MH) composite nanofibrous mats as a biocompatible and antimicrobial wound dressing.
32240741	3	68	theme	MH	414:415	arg1	quantities					400:409	different quantities	390:409	different quantities of MH	390:415	CA mats with different quantities of MH were developed by electrospinning.
32240741	7	69	theme	composite	718:726	arg1	mats					746:749	the electrospun composite CA-MH nanofibrous mats	702:749	the electrospun composite CA-MH nanofibrous mats	702:749	Inclusion of MH in the electrospun composite CA-MH nanofibrous mats shows high efficacy to prevent bacterial growth on the wound surface.
32240741	9	70	theme	high	1187:1190	arg1	cytocompatibility					1192:1208	high cytocompatibility	1187:1208	high cytocompatibility to NIH 3T3 cell line	1187:1229	Besides, the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m2/day, suitable for wound breathability and the mats show high cytocompatibility to NIH 3T3 cell line in in vitro testing, proving to be effective for promoting wound healing.
32240741	8	71	from	content	971:977	arg1	fiber					986:990	the fiber	982:990	the fiber	982:990	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	8	72	theme	MH	825:826	arg1	mats					848:851	The MH loaded CA nanofiber mats	821:851	The MH loaded CA nanofiber mats	821:851	The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution.
32240741	7	73	theme	CA-MH	728:732	arg1	mats					746:749	the electrospun composite CA-MH nanofibrous mats	702:749	the electrospun composite CA-MH nanofibrous mats	702:749	Inclusion of MH in the electrospun composite CA-MH nanofibrous mats shows high efficacy to prevent bacterial growth on the wound surface.
34806923	0	0	theme	drug	103:106	arg1	system					117:122	drug delivery system	103:122	drug delivery system	103:122	Improving the 6-Aminopenicillanic acid release process using vermiculite-alginate biocomposite bead on drug delivery system.
34806923	5	1	theme	drug	812:815	arg1	absorption					817:826	drug absorption	812:826	drug absorption on VMT-Alg	812:837	The equilibrium and Kinetic studies (chemical reaction and diffusion-based models) indicated that drug absorption on VMT-Alg is homogeneous with chemical interaction.
34806923	3	2	theme	response	511:518	arg1	RSM					536:538	RSM	536:538	RSM	536:538	Optimization attempts were explored via the response surface method (RSM) to best predict the actual amount of compound.
34806923	3	2	theme	response	511:518	arg1	method					528:533	the response surface method	507:533	the response surface method (RSM)	507:539	Optimization attempts were explored via the response surface method (RSM) to best predict the actual amount of compound.
34806923	6	3	theme	cross-linker	896:907	arg1	concentration					917:929	cross-linker (CaCl2) concentration	896:929	cross-linker (CaCl2) concentration	896:929	An increase in cross-linker (CaCl2) concentration leads to improvement in the drug encapsulation efficiency while having no significant effect on loading efficiency.
34806923	3	4	theme	surface	520:526	arg1	RSM					536:538	RSM	536:538	RSM	536:538	Optimization attempts were explored via the response surface method (RSM) to best predict the actual amount of compound.
34806923	3	4	theme	surface	520:526	arg1	method					528:533	the response surface method	507:533	the response surface method (RSM)	507:539	Optimization attempts were explored via the response surface method (RSM) to best predict the actual amount of compound.
34806923	7	5	theme	synthesized	1174:1184	arg1	drug					1186:1189	the synthesized drug	1170:1189	the synthesized drug with Alg	1170:1198	The in-vitro release of the pure drug shows a rapid burst release followed by 100% cumulative release within 6 h. Whereas, the synthesized drug with Alg substantially showed less release of 43% after 8 h. Release experiments revealed that the presence of the CaCl2 delayed the release of the 6-APA less than 35% after 12 h.
34806923	8	6	theme	kinetic	1483:1489	arg1	exponent					1491:1498	the kinetic exponent	1479:1498	the kinetic exponent (n < 0.5)	1479:1508	The kinetic release of 6-APA is followed by the Korsmeyer-Peppas model based on Fick's law mechanism due to the kinetic exponent (n < 0.5).
34806923	8	6	theme	kinetic	1483:1489	arg1	n < 0.5					1501:1507	n < 0.5	1501:1507	n < 0.5	1501:1507	The kinetic release of 6-APA is followed by the Korsmeyer-Peppas model based on Fick's law mechanism due to the kinetic exponent (n < 0.5).
34806923	7	7	theme	rapid	1093:1097	arg1	release					1105:1111	a rapid burst release	1091:1111	a rapid burst release followed by 100% cumulative release within 6 h.	1091:1159	The in-vitro release of the pure drug shows a rapid burst release followed by 100% cumulative release within 6 h. Whereas, the synthesized drug with Alg substantially showed less release of 43% after 8 h. Release experiments revealed that the presence of the CaCl2 delayed the release of the 6-APA less than 35% after 12 h.
34806923	10	8	theme	composites	1640:1649	arg1	activities					1626:1635	The antibacterial activities	1608:1635	The antibacterial activities of composites	1608:1649	The antibacterial activities of composites were evaluated by the halo of no growth.
34806923	0	9	theme	delivery	108:115	arg1	system					117:122	drug delivery system	103:122	drug delivery system	103:122	Improving the 6-Aminopenicillanic acid release process using vermiculite-alginate biocomposite bead on drug delivery system.
34806923	6	10	from	effect	1017:1022	arg1	efficiency					1035:1044	loading efficiency	1027:1044	loading efficiency	1027:1044	An increase in cross-linker (CaCl2) concentration leads to improvement in the drug encapsulation efficiency while having no significant effect on loading efficiency.
34806923	10	11	theme	growth	1684:1689	arg1	halo					1673:1676	the halo	1669:1676	the halo of no growth	1669:1689	The antibacterial activities of composites were evaluated by the halo of no growth.
34806923	6	12	theme	encapsulation	964:976	arg1	efficiency					978:987	the drug encapsulation efficiency	955:987	the drug encapsulation efficiency	955:987	An increase in cross-linker (CaCl2) concentration leads to improvement in the drug encapsulation efficiency while having no significant effect on loading efficiency.
34806923	6	13	from	increase	884:891	arg1	concentration					917:929	cross-linker (CaCl2) concentration	896:929	cross-linker (CaCl2) concentration	896:929	An increase in cross-linker (CaCl2) concentration leads to improvement in the drug encapsulation efficiency while having no significant effect on loading efficiency.
34806923	2	14	theme	composites	354:363	arg1	Characterization					322:337	The Characterization	318:337	The Characterization of synthesized composites	318:363	The Characterization of synthesized composites was conducted by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analyses.
34806923	2	15	dep	Fourier	382:388	arg1	transform					390:398	transform	390:398	transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analyses	390:464	The Characterization of synthesized composites was conducted by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analyses.
34806923	11	16	theme	VMT-Alg-6APA	1720:1731	arg1	composite					1733:1741	the VMT-Alg-6APA composite	1716:1741	the VMT-Alg-6APA composite	1716:1741	The results showed that the VMT-Alg-6APA composite had strong activity against Gram-positive and Gram-negative bacteria.
34806923	7	17	theme	100	1125:1127	arg1	%					1128:1128	%	1128:1128	%	1128:1128	The in-vitro release of the pure drug shows a rapid burst release followed by 100% cumulative release within 6 h. Whereas, the synthesized drug with Alg substantially showed less release of 43% after 8 h. Release experiments revealed that the presence of the CaCl2 delayed the release of the 6-APA less than 35% after 12 h.
34806923	7	18	theme	%	1128:1128	arg1	release					1141:1147	100% cumulative release	1125:1147	100% cumulative release within 6 h.	1125:1159	The in-vitro release of the pure drug shows a rapid burst release followed by 100% cumulative release within 6 h. Whereas, the synthesized drug with Alg substantially showed less release of 43% after 8 h. Release experiments revealed that the presence of the CaCl2 delayed the release of the 6-APA less than 35% after 12 h.
34806923	9	19	theme	24 h	1563:1566	arg1	exposure					1568:1575	24 h exposure	1563:1575	24 h exposure	1563:1575	All studied composites antibacterial activity after 24 h exposure against E. Coli and S. aureus.
34806923	1	20	theme	-alginate	182:190	arg1	composites					198:207	vermiculite (VMT)-alginate (Alg) composites	165:207	vermiculite (VMT)-alginate (Alg) composites with different cross-linker concentrations (CaCl2)	165:258	The present study deals with developing vermiculite (VMT)-alginate (Alg) composites with different cross-linker concentrations (CaCl2) to deliver the controlled 6-aminopenicillin acid (6-APA).
34806923	8	21	theme	due	1472:1474	arg1	mechanism					1462:1470	Fick's law mechanism	1451:1470	Fick's law mechanism due to the kinetic exponent (n < 0.5)	1451:1508	The kinetic release of 6-APA is followed by the Korsmeyer-Peppas model based on Fick's law mechanism due to the kinetic exponent (n < 0.5).
34806923	7	22	theme	pure	1075:1078	arg1	drug					1080:1083	the pure drug	1071:1083	the pure drug	1071:1083	The in-vitro release of the pure drug shows a rapid burst release followed by 100% cumulative release within 6 h. Whereas, the synthesized drug with Alg substantially showed less release of 43% after 8 h. Release experiments revealed that the presence of the CaCl2 delayed the release of the 6-APA less than 35% after 12 h.
34806923	6	23	theme	drug	959:962	arg1	efficiency					978:987	the drug encapsulation efficiency	955:987	the drug encapsulation efficiency	955:987	An increase in cross-linker (CaCl2) concentration leads to improvement in the drug encapsulation efficiency while having no significant effect on loading efficiency.
34806923	3	24	theme	actual	561:566	arg1	compound					578:585	compound	578:585	compound	578:585	Optimization attempts were explored via the response surface method (RSM) to best predict the actual amount of compound.
34806923	3	24	theme	actual	561:566	arg1	amount					568:573	the actual amount	557:573	the actual amount of compound	557:585	Optimization attempts were explored via the response surface method (RSM) to best predict the actual amount of compound.
34806923	8	25	theme	law	1458:1460	arg1	mechanism					1462:1470	Fick's law mechanism	1451:1470	Fick's law mechanism due to the kinetic exponent (n < 0.5)	1451:1508	The kinetic release of 6-APA is followed by the Korsmeyer-Peppas model based on Fick's law mechanism due to the kinetic exponent (n < 0.5).
34806923	10	26	theme	no	1681:1682	arg1	growth					1684:1689	no growth	1681:1689	no growth	1681:1689	The antibacterial activities of composites were evaluated by the halo of no growth.
34806923	1	27	theme	controlled	275:284	arg1	acid					304:307	the controlled 6-aminopenicillin acid	271:307	the controlled 6-aminopenicillin acid (6-APA)	271:315	The present study deals with developing vermiculite (VMT)-alginate (Alg) composites with different cross-linker concentrations (CaCl2) to deliver the controlled 6-aminopenicillin acid (6-APA).
34806923	1	27	theme	controlled	275:284	arg1	6-APA					310:314	6-APA	310:314	6-APA	310:314	The present study deals with developing vermiculite (VMT)-alginate (Alg) composites with different cross-linker concentrations (CaCl2) to deliver the controlled 6-aminopenicillin acid (6-APA).
34806923	7	28	theme	drug	1080:1083	arg1	release					1060:1066	The in-vitro release	1047:1066	The in-vitro release of the pure drug	1047:1083	The in-vitro release of the pure drug shows a rapid burst release followed by 100% cumulative release within 6 h. Whereas, the synthesized drug with Alg substantially showed less release of 43% after 8 h. Release experiments revealed that the presence of the CaCl2 delayed the release of the 6-APA less than 35% after 12 h.
34806923	5	29	theme	chemical	859:866	arg1	interaction					868:878	chemical interaction	859:878	chemical interaction	859:878	The equilibrium and Kinetic studies (chemical reaction and diffusion-based models) indicated that drug absorption on VMT-Alg is homogeneous with chemical interaction.
34806923	0	30	theme	acid	34:37	arg1	process					47:53	the 6-Aminopenicillanic acid release process	10:53	the 6-Aminopenicillanic acid release process using vermiculite-alginate biocomposite bead on drug delivery system	10:122	Improving the 6-Aminopenicillanic acid release process using vermiculite-alginate biocomposite bead on drug delivery system.
34806923	1	31	theme	6-aminopenicillin	286:302	arg1	acid					304:307	the controlled 6-aminopenicillin acid	271:307	the controlled 6-aminopenicillin acid (6-APA)	271:315	The present study deals with developing vermiculite (VMT)-alginate (Alg) composites with different cross-linker concentrations (CaCl2) to deliver the controlled 6-aminopenicillin acid (6-APA).
34806923	1	31	theme	6-aminopenicillin	286:302	arg1	6-APA					310:314	6-APA	310:314	6-APA	310:314	The present study deals with developing vermiculite (VMT)-alginate (Alg) composites with different cross-linker concentrations (CaCl2) to deliver the controlled 6-aminopenicillin acid (6-APA).
34806923	5	32	dep	studies	742:748	arg1	reaction					760:767	chemical reaction	751:767	chemical reaction	751:767	The equilibrium and Kinetic studies (chemical reaction and diffusion-based models) indicated that drug absorption on VMT-Alg is homogeneous with chemical interaction.
34806923	5	32	dep	studies	742:748	arg1	models					789:794	diffusion-based models	773:794	diffusion-based models	773:794	The equilibrium and Kinetic studies (chemical reaction and diffusion-based models) indicated that drug absorption on VMT-Alg is homogeneous with chemical interaction.
34806923	3	33	theme	compound	578:585	arg1	compound					578:585	compound	578:585	compound	578:585	Optimization attempts were explored via the response surface method (RSM) to best predict the actual amount of compound.
34806923	3	33	theme	compound	578:585	arg1	amount					568:573	the actual amount	557:573	the actual amount of compound	557:585	Optimization attempts were explored via the response surface method (RSM) to best predict the actual amount of compound.
34806923	11	34	theme	Gram-negative	1789:1801	arg1	bacteria					1803:1810	Gram-positive and Gram-negative bacteria	1771:1810	Gram-positive and Gram-negative bacteria	1771:1810	The results showed that the VMT-Alg-6APA composite had strong activity against Gram-positive and Gram-negative bacteria.
34806923	2	35	theme	X-ray	433:437	arg1	XRD					452:454	XRD	452:454	XRD	452:454	The Characterization of synthesized composites was conducted by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analyses.
34806923	2	35	theme	X-ray	433:437	arg1	diffraction					439:449	X-ray diffraction	433:449	X-ray diffraction (XRD)	433:455	The Characterization of synthesized composites was conducted by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analyses.
34806923	0	36	theme	6-Aminopenicillanic	14:32	arg1	process					47:53	the 6-Aminopenicillanic acid release process	10:53	the 6-Aminopenicillanic acid release process using vermiculite-alginate biocomposite bead on drug delivery system	10:122	Improving the 6-Aminopenicillanic acid release process using vermiculite-alginate biocomposite bead on drug delivery system.
34806923	6	37	from	improvement	940:950	arg1	efficiency					978:987	the drug encapsulation efficiency	955:987	the drug encapsulation efficiency	955:987	An increase in cross-linker (CaCl2) concentration leads to improvement in the drug encapsulation efficiency while having no significant effect on loading efficiency.
34806923	8	38	theme	6-APA	1394:1398	arg1	release					1383:1389	The kinetic release	1371:1389	The kinetic release of 6-APA	1371:1398	The kinetic release of 6-APA is followed by the Korsmeyer-Peppas model based on Fick's law mechanism due to the kinetic exponent (n < 0.5).
34806923	7	39	theme	cumulative	1130:1139	arg1	release					1141:1147	100% cumulative release	1125:1147	100% cumulative release within 6 h.	1125:1159	The in-vitro release of the pure drug shows a rapid burst release followed by 100% cumulative release within 6 h. Whereas, the synthesized drug with Alg substantially showed less release of 43% after 8 h. Release experiments revealed that the presence of the CaCl2 delayed the release of the 6-APA less than 35% after 12 h.
34806923	5	40	theme	chemical	751:758	arg1	reaction					760:767	chemical reaction	751:767	chemical reaction	751:767	The equilibrium and Kinetic studies (chemical reaction and diffusion-based models) indicated that drug absorption on VMT-Alg is homogeneous with chemical interaction.
34806923	7	41	theme	Release	1252:1258	arg1	experiments					1260:1270	8 h. Release experiments	1247:1270	8 h. Release experiments	1247:1270	The in-vitro release of the pure drug shows a rapid burst release followed by 100% cumulative release within 6 h. Whereas, the synthesized drug with Alg substantially showed less release of 43% after 8 h. Release experiments revealed that the presence of the CaCl2 delayed the release of the 6-APA less than 35% after 12 h.
34806923	7	42	theme	in-vitro	1051:1058	arg1	release					1060:1066	The in-vitro release	1047:1066	The in-vitro release of the pure drug	1047:1083	The in-vitro release of the pure drug shows a rapid burst release followed by 100% cumulative release within 6 h. Whereas, the synthesized drug with Alg substantially showed less release of 43% after 8 h. Release experiments revealed that the presence of the CaCl2 delayed the release of the 6-APA less than 35% after 12 h.
34806923	8	43	theme	Korsmeyer-Peppas	1419:1434	arg1	model					1436:1440	the Korsmeyer-Peppas model	1415:1440	the Korsmeyer-Peppas model based on Fick's law mechanism due to the kinetic exponent (n < 0.5)	1415:1508	The kinetic release of 6-APA is followed by the Korsmeyer-Peppas model based on Fick's law mechanism due to the kinetic exponent (n < 0.5).
34806923	9	44	theme	composites	1523:1532	arg1	activity					1548:1555	composites antibacterial activity	1523:1555	composites antibacterial activity after 24 h exposure against E. Coli and S. aureus	1523:1605	All studied composites antibacterial activity after 24 h exposure against E. Coli and S. aureus.
34806923	0	45	theme	release	39:45	arg1	process					47:53	the 6-Aminopenicillanic acid release process	10:53	the 6-Aminopenicillanic acid release process using vermiculite-alginate biocomposite bead on drug delivery system	10:122	Improving the 6-Aminopenicillanic acid release process using vermiculite-alginate biocomposite bead on drug delivery system.
34806923	1	46	with	composites	198:207	arg1	CaCl2					253:257	CaCl2	253:257	CaCl2	253:257	The present study deals with developing vermiculite (VMT)-alginate (Alg) composites with different cross-linker concentrations (CaCl2) to deliver the controlled 6-aminopenicillin acid (6-APA).
34806923	1	46	with	composites	198:207	arg1	concentrations					237:250	different cross-linker concentrations	214:250	different cross-linker concentrations (CaCl2)	214:258	The present study deals with developing vermiculite (VMT)-alginate (Alg) composites with different cross-linker concentrations (CaCl2) to deliver the controlled 6-aminopenicillin acid (6-APA).
34806923	6	47	theme	significant	1005:1015	arg1	effect					1017:1022	no significant effect	1002:1022	no significant effect on loading efficiency	1002:1044	An increase in cross-linker (CaCl2) concentration leads to improvement in the drug encapsulation efficiency while having no significant effect on loading efficiency.
34806923	7	48	theme	6-APA	1339:1343	arg1	release					1324:1330	the release	1320:1330	the release of the 6-APA	1320:1343	The in-vitro release of the pure drug shows a rapid burst release followed by 100% cumulative release within 6 h. Whereas, the synthesized drug with Alg substantially showed less release of 43% after 8 h. Release experiments revealed that the presence of the CaCl2 delayed the release of the 6-APA less than 35% after 12 h.
34806923	1	49	theme	different	214:222	arg1	CaCl2					253:257	CaCl2	253:257	CaCl2	253:257	The present study deals with developing vermiculite (VMT)-alginate (Alg) composites with different cross-linker concentrations (CaCl2) to deliver the controlled 6-aminopenicillin acid (6-APA).
34806923	1	49	theme	different	214:222	arg1	concentrations					237:250	different cross-linker concentrations	214:250	different cross-linker concentrations (CaCl2)	214:258	The present study deals with developing vermiculite (VMT)-alginate (Alg) composites with different cross-linker concentrations (CaCl2) to deliver the controlled 6-aminopenicillin acid (6-APA).
34806923	0	50	theme	vermiculite-alginate	61:80	arg1	bead					95:98	vermiculite-alginate biocomposite bead	61:98	vermiculite-alginate biocomposite bead	61:98	Improving the 6-Aminopenicillanic acid release process using vermiculite-alginate biocomposite bead on drug delivery system.
34806923	11	51	theme	Gram-positive	1771:1783	arg1	bacteria					1803:1810	Gram-positive and Gram-negative bacteria	1771:1810	Gram-positive and Gram-negative bacteria	1771:1810	The results showed that the VMT-Alg-6APA composite had strong activity against Gram-positive and Gram-negative bacteria.
34806923	5	52	theme	Kinetic	734:740	arg1	studies					742:748	Kinetic studies	734:748	Kinetic studies (chemical reaction and diffusion-based models)	734:795	The equilibrium and Kinetic studies (chemical reaction and diffusion-based models) indicated that drug absorption on VMT-Alg is homogeneous with chemical interaction.
34806923	9	53	theme	antibacterial	1534:1546	arg1	activity					1548:1555	composites antibacterial activity	1523:1555	composites antibacterial activity after 24 h exposure against E. Coli and S. aureus	1523:1605	All studied composites antibacterial activity after 24 h exposure against E. Coli and S. aureus.
34806923	1	54	theme	present	129:135	arg1	study					137:141	The present study	125:141	The present study	125:141	The present study deals with developing vermiculite (VMT)-alginate (Alg) composites with different cross-linker concentrations (CaCl2) to deliver the controlled 6-aminopenicillin acid (6-APA).
34806923	5	55	theme	diffusion-based	773:787	arg1	models					789:794	diffusion-based models	773:794	diffusion-based models	773:794	The equilibrium and Kinetic studies (chemical reaction and diffusion-based models) indicated that drug absorption on VMT-Alg is homogeneous with chemical interaction.
34806923	11	56	theme	strong	1747:1752	arg1	activity					1754:1761	strong activity	1747:1761	strong activity	1747:1761	The results showed that the VMT-Alg-6APA composite had strong activity against Gram-positive and Gram-negative bacteria.
34806923	1	57	dep	-alginate	182:190	arg1	Alg					193:195	Alg	193:195	Alg	193:195	The present study deals with developing vermiculite (VMT)-alginate (Alg) composites with different cross-linker concentrations (CaCl2) to deliver the controlled 6-aminopenicillin acid (6-APA).
34806923	7	58	theme	8 h.	1247:1250	arg1	experiments					1260:1270	8 h. Release experiments	1247:1270	8 h. Release experiments	1247:1270	The in-vitro release of the pure drug shows a rapid burst release followed by 100% cumulative release within 6 h. Whereas, the synthesized drug with Alg substantially showed less release of 43% after 8 h. Release experiments revealed that the presence of the CaCl2 delayed the release of the 6-APA less than 35% after 12 h.
34806923	2	59	theme	synthesized	342:352	arg1	composites					354:363	synthesized composites	342:363	synthesized composites	342:363	The Characterization of synthesized composites was conducted by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analyses.
34806923	7	60	with	drug	1186:1189	arg1	Alg					1196:1198	Alg	1196:1198	Alg	1196:1198	The in-vitro release of the pure drug shows a rapid burst release followed by 100% cumulative release within 6 h. Whereas, the synthesized drug with Alg substantially showed less release of 43% after 8 h. Release experiments revealed that the presence of the CaCl2 delayed the release of the 6-APA less than 35% after 12 h.
34806923	8	61	theme	kinetic	1375:1381	arg1	release					1383:1389	The kinetic release	1371:1389	The kinetic release of 6-APA	1371:1398	The kinetic release of 6-APA is followed by the Korsmeyer-Peppas model based on Fick's law mechanism due to the kinetic exponent (n < 0.5).
34806923	5	62	from	absorption	817:826	arg1	VMT-Alg					831:837	VMT-Alg	831:837	VMT-Alg	831:837	The equilibrium and Kinetic studies (chemical reaction and diffusion-based models) indicated that drug absorption on VMT-Alg is homogeneous with chemical interaction.
34806923	4	63	theme	other	701:705	arg1	clays					707:711	other clays	701:711	other clays	701:711	The adsorption capacity of 6-APA onto this adsorbent was found to be 208.33 mg/g, which was higher than that for other clays.
34806923	11	64	contain	had	1743:1745	arg1	composite					1733:1741	the VMT-Alg-6APA composite	1716:1741	the VMT-Alg-6APA composite	1716:1741	The results showed that the VMT-Alg-6APA composite had strong activity against Gram-positive and Gram-negative bacteria.
34806923	11	64	contain	had	1743:1745	arg2	activity					1754:1761	strong activity	1747:1761	strong activity	1747:1761	The results showed that the VMT-Alg-6APA composite had strong activity against Gram-positive and Gram-negative bacteria.
34806923	1	65	theme	cross-linker	224:235	arg1	CaCl2					253:257	CaCl2	253:257	CaCl2	253:257	The present study deals with developing vermiculite (VMT)-alginate (Alg) composites with different cross-linker concentrations (CaCl2) to deliver the controlled 6-aminopenicillin acid (6-APA).
34806923	1	65	theme	cross-linker	224:235	arg1	concentrations					237:250	different cross-linker concentrations	214:250	different cross-linker concentrations (CaCl2)	214:258	The present study deals with developing vermiculite (VMT)-alginate (Alg) composites with different cross-linker concentrations (CaCl2) to deliver the controlled 6-aminopenicillin acid (6-APA).
34806923	5	66	with	homogeneous	842:852	arg1	interaction					868:878	chemical interaction	859:878	chemical interaction	859:878	The equilibrium and Kinetic studies (chemical reaction and diffusion-based models) indicated that drug absorption on VMT-Alg is homogeneous with chemical interaction.
34806923	2	67	dep	transform	390:398	arg1	infrared					400:407	infrared	400:407	transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analyses	390:464	The Characterization of synthesized composites was conducted by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analyses.
34806923	10	68	theme	antibacterial	1612:1624	arg1	activities					1626:1635	The antibacterial activities	1608:1635	The antibacterial activities of composites	1608:1649	The antibacterial activities of composites were evaluated by the halo of no growth.
34806923	4	69	theme	adsorption	592:601	arg1	capacity					603:610	The adsorption capacity	588:610	The adsorption capacity of 6-APA onto this adsorbent	588:639	The adsorption capacity of 6-APA onto this adsorbent was found to be 208.33 mg/g, which was higher than that for other clays.
34806923	4	69	theme	adsorption	592:601	arg1	208.33 mg/g					657:667	208.33 mg/g	657:667	208.33 mg/g	657:667	The adsorption capacity of 6-APA onto this adsorbent was found to be 208.33 mg/g, which was higher than that for other clays.
34806923	6	70	theme	loading	1027:1033	arg1	efficiency					1035:1044	loading efficiency	1027:1044	loading efficiency	1027:1044	An increase in cross-linker (CaCl2) concentration leads to improvement in the drug encapsulation efficiency while having no significant effect on loading efficiency.
34806923	3	71	theme	Optimization	467:478	arg1	attempts					480:487	Optimization attempts	467:487	Optimization attempts	467:487	Optimization attempts were explored via the response surface method (RSM) to best predict the actual amount of compound.
34806923	7	72	theme	CaCl2	1306:1310	arg1	presence					1290:1297	the presence	1286:1297	the presence of the CaCl2	1286:1310	The in-vitro release of the pure drug shows a rapid burst release followed by 100% cumulative release within 6 h. Whereas, the synthesized drug with Alg substantially showed less release of 43% after 8 h. Release experiments revealed that the presence of the CaCl2 delayed the release of the 6-APA less than 35% after 12 h.
34806923	4	73	theme	6-APA	615:619	arg1	capacity					603:610	The adsorption capacity	588:610	The adsorption capacity of 6-APA onto this adsorbent	588:639	The adsorption capacity of 6-APA onto this adsorbent was found to be 208.33 mg/g, which was higher than that for other clays.
34806923	4	73	theme	6-APA	615:619	arg1	208.33 mg/g					657:667	208.33 mg/g	657:667	208.33 mg/g	657:667	The adsorption capacity of 6-APA onto this adsorbent was found to be 208.33 mg/g, which was higher than that for other clays.
34806923	0	74	theme	biocomposite	82:93	arg1	bead					95:98	vermiculite-alginate biocomposite bead	61:98	vermiculite-alginate biocomposite bead	61:98	Improving the 6-Aminopenicillanic acid release process using vermiculite-alginate biocomposite bead on drug delivery system.
34806923	6	75	theme	CaCl2	910:914	arg1	concentration					917:929	cross-linker (CaCl2) concentration	896:929	cross-linker (CaCl2) concentration	896:929	An increase in cross-linker (CaCl2) concentration leads to improvement in the drug encapsulation efficiency while having no significant effect on loading efficiency.
34806923	2	76	dep	spectroscopy	409:420	arg1	analyses					457:464	analyses	457:464	analyses	457:464	The Characterization of synthesized composites was conducted by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analyses.
34806923	7	77	theme	%	1239:1239	arg1	release					1226:1232	less release	1221:1232	less release of 43%	1221:1239	The in-vitro release of the pure drug shows a rapid burst release followed by 100% cumulative release within 6 h. Whereas, the synthesized drug with Alg substantially showed less release of 43% after 8 h. Release experiments revealed that the presence of the CaCl2 delayed the release of the 6-APA less than 35% after 12 h.
34806923	7	78	dep	%	1357:1357	arg1	12 h					1365:1368	12 h	1365:1368	12 h	1365:1368	The in-vitro release of the pure drug shows a rapid burst release followed by 100% cumulative release within 6 h. Whereas, the synthesized drug with Alg substantially showed less release of 43% after 8 h. Release experiments revealed that the presence of the CaCl2 delayed the release of the 6-APA less than 35% after 12 h.
34806923	7	79	theme	burst	1099:1103	arg1	release					1105:1111	a rapid burst release	1091:1111	a rapid burst release followed by 100% cumulative release within 6 h.	1091:1159	The in-vitro release of the pure drug shows a rapid burst release followed by 100% cumulative release within 6 h. Whereas, the synthesized drug with Alg substantially showed less release of 43% after 8 h. Release experiments revealed that the presence of the CaCl2 delayed the release of the 6-APA less than 35% after 12 h.
32679329	9	0	theme	prenati	1568:1574	arg1	fillets					1576:1582	Schizothorax prenati fillets	1555:1582	Schizothorax prenati fillets	1555:1582	In conclusion, our work shows that CCN/zein/PS composite film holds promise as a potential bioactive packaging material for Schizothorax prenati fillets.
32679329	8	1	theme	prenati	1366:1372	arg1	fillets					1374:1380	the Schizothorax prenati fillets	1349:1380	the Schizothorax prenati fillets	1349:1380	The CCN/zein/PS composite film delayed physicochemical changes in the Schizothorax prenati fillets and prolonged their shelf life by up to 15 days.
32679329	5	2	theme	mechanical	703:712	arg1	permeability					764:775	water vapor permeability	752:775	water vapor permeability (WVP)	752:781	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	2	theme	mechanical	703:712	arg1	properties					714:723	the mechanical properties	699:723	the mechanical properties	699:723	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	2	theme	mechanical	703:712	arg1	permeability					733:744	oxygen permeability	726:744	oxygen permeability (OP)	726:749	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	2	theme	mechanical	703:712	arg1	efficiency					801:810	relative release efficiency	784:810	relative release efficiency	784:810	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	2	theme	mechanical	703:712	arg1	properties					841:850	DPPH radical scavenging properties	817:850	DPPH radical scavenging properties	817:850	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	7	3	theme	composite	1036:1044	arg1	film					1046:1049	the composite film	1032:1049	the composite film	1032:1049	Further, we evaluated the preservative efficacy of the composite film on Schizothorax prenati fillets by measuring pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS), hardness, microbial counts, organoleptic characteristics, and other fillet quality parameters.
32679329	2	4	theme	particle	333:340	arg1	size					342:345	particle size	333:345	particle size	333:345	The CCN film was characterized for encapsulation efficiency, particle size, zeta potential, polydispersity index (PDI), relative release, and DPPH radical scavenging test.
32679329	0	5	theme	prenati	109:115	arg1	preservation					124:135	Schizothorax prenati fillet preservation	96:135	Schizothorax prenati fillet preservation	96:135	Preparation of chitosan/curcumin nanoparticles based zein and potato starch composite films for Schizothorax prenati fillet preservation.
32679329	7	6	theme	quality	1263:1269	arg1	parameters					1271:1280	other fillet quality parameters	1250:1280	other fillet quality parameters	1250:1280	Further, we evaluated the preservative efficacy of the composite film on Schizothorax prenati fillets by measuring pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS), hardness, microbial counts, organoleptic characteristics, and other fillet quality parameters.
32679329	9	7	theme	potential	1512:1520	arg1	material					1542:1549	a potential bioactive packaging material	1510:1549	a potential bioactive packaging material for Schizothorax prenati fillets	1510:1582	In conclusion, our work shows that CCN/zein/PS composite film holds promise as a potential bioactive packaging material for Schizothorax prenati fillets.
32679329	9	7	theme	potential	1512:1520	arg1	promise					1499:1505	promise	1499:1505	promise	1499:1505	In conclusion, our work shows that CCN/zein/PS composite film holds promise as a potential bioactive packaging material for Schizothorax prenati fillets.
32679329	5	8	theme	films	869:873	arg1	permeability					764:775	water vapor permeability	752:775	water vapor permeability (WVP)	752:781	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	8	theme	films	869:873	arg1	properties					714:723	the mechanical properties	699:723	the mechanical properties	699:723	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	8	theme	films	869:873	arg1	permeability					733:744	oxygen permeability	726:744	oxygen permeability (OP)	726:749	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	8	theme	films	869:873	arg1	efficiency					801:810	relative release efficiency	784:810	relative release efficiency	784:810	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	8	theme	films	869:873	arg1	properties					841:850	DPPH radical scavenging properties	817:850	DPPH radical scavenging properties	817:850	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	0	9	theme	Schizothorax	96:107	arg1	preservation					124:135	Schizothorax prenati fillet preservation	96:135	Schizothorax prenati fillet preservation	96:135	Preparation of chitosan/curcumin nanoparticles based zein and potato starch composite films for Schizothorax prenati fillet preservation.
32679329	7	10	theme	thiobarbituric	1139:1152	arg1	TBARS					1180:1184	TBARS	1180:1184	TBARS	1180:1184	Further, we evaluated the preservative efficacy of the composite film on Schizothorax prenati fillets by measuring pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS), hardness, microbial counts, organoleptic characteristics, and other fillet quality parameters.
32679329	7	10	theme	thiobarbituric	1139:1152	arg1	substances					1168:1177	thiobarbituric acid-reactive substances	1139:1177	thiobarbituric acid-reactive substances (TBARS)	1139:1185	Further, we evaluated the preservative efficacy of the composite film on Schizothorax prenati fillets by measuring pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS), hardness, microbial counts, organoleptic characteristics, and other fillet quality parameters.
32679329	5	11	theme	different	878:886	arg1	ratios					893:898	different mass ratios	878:898	different mass ratios	878:898	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	8	12	from	changes	1338:1344	arg1	fillets					1374:1380	the Schizothorax prenati fillets	1349:1380	the Schizothorax prenati fillets	1349:1380	The CCN/zein/PS composite film delayed physicochemical changes in the Schizothorax prenati fillets and prolonged their shelf life by up to 15 days.
32679329	7	13	theme	acid-reactive	1154:1166	arg1	TBARS					1180:1184	TBARS	1180:1184	TBARS	1180:1184	Further, we evaluated the preservative efficacy of the composite film on Schizothorax prenati fillets by measuring pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS), hardness, microbial counts, organoleptic characteristics, and other fillet quality parameters.
32679329	7	13	theme	acid-reactive	1154:1166	arg1	substances					1168:1177	thiobarbituric acid-reactive substances	1139:1177	thiobarbituric acid-reactive substances (TBARS)	1139:1185	Further, we evaluated the preservative efficacy of the composite film on Schizothorax prenati fillets by measuring pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS), hardness, microbial counts, organoleptic characteristics, and other fillet quality parameters.
32679329	5	14	theme	vapor	758:762	arg1	permeability					764:775	water vapor permeability	752:775	water vapor permeability (WVP)	752:781	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	14	theme	vapor	758:762	arg1	properties					714:723	the mechanical properties	699:723	the mechanical properties	699:723	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	14	theme	vapor	758:762	arg1	WVP					778:780	WVP	778:780	WVP	778:780	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	15	theme	mass	888:891	arg1	ratios					893:898	different mass ratios	878:898	different mass ratios	878:898	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	4	16	theme	CCN/zein/PS	613:623	arg1	round					646:650	round	646:650	round	646:650	The CCN/zein/PS composite films were round, smooth, and compact.
32679329	4	16	theme	CCN/zein/PS	613:623	arg1	films					635:639	The CCN/zein/PS composite films	609:639	The CCN/zein/PS composite films	609:639	The CCN/zein/PS composite films were round, smooth, and compact.
32679329	0	17	theme	fillet	117:122	arg1	preservation					124:135	Schizothorax prenati fillet preservation	96:135	Schizothorax prenati fillet preservation	96:135	Preparation of chitosan/curcumin nanoparticles based zein and potato starch composite films for Schizothorax prenati fillet preservation.
32679329	7	18	theme	preservative	1007:1018	arg1	efficacy					1020:1027	the preservative efficacy	1003:1027	the preservative efficacy of the composite film	1003:1049	Further, we evaluated the preservative efficacy of the composite film on Schizothorax prenati fillets by measuring pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS), hardness, microbial counts, organoleptic characteristics, and other fillet quality parameters.
32679329	9	19	theme	packaging	1532:1540	arg1	material					1542:1549	a potential bioactive packaging material	1510:1549	a potential bioactive packaging material for Schizothorax prenati fillets	1510:1582	In conclusion, our work shows that CCN/zein/PS composite film holds promise as a potential bioactive packaging material for Schizothorax prenati fillets.
32679329	9	19	theme	packaging	1532:1540	arg1	promise					1499:1505	promise	1499:1505	promise	1499:1505	In conclusion, our work shows that CCN/zein/PS composite film holds promise as a potential bioactive packaging material for Schizothorax prenati fillets.
32679329	5	20	theme	ratios	893:898	arg1	films					869:873	the composite films	855:873	the composite films of different mass ratios	855:898	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	2	21	theme	polydispersity	364:377	arg1	PDI					386:388	PDI	386:388	PDI	386:388	The CCN film was characterized for encapsulation efficiency, particle size, zeta potential, polydispersity index (PDI), relative release, and DPPH radical scavenging test.
32679329	2	21	theme	polydispersity	364:377	arg1	index					379:383	polydispersity index	364:383	polydispersity index (PDI)	364:389	The CCN film was characterized for encapsulation efficiency, particle size, zeta potential, polydispersity index (PDI), relative release, and DPPH radical scavenging test.
32679329	9	22	theme	CCN/zein/PS	1466:1476	arg1	film					1488:1491	CCN/zein/PS composite film	1466:1491	CCN/zein/PS composite film	1466:1491	In conclusion, our work shows that CCN/zein/PS composite film holds promise as a potential bioactive packaging material for Schizothorax prenati fillets.
32679329	9	23	theme	composite	1478:1486	arg1	film					1488:1491	CCN/zein/PS composite film	1466:1491	CCN/zein/PS composite film	1466:1491	In conclusion, our work shows that CCN/zein/PS composite film holds promise as a potential bioactive packaging material for Schizothorax prenati fillets.
32679329	1	24	theme	zein/potato	177:187	arg1	PS					197:198	PS	197:198	PS	197:198	The aim of this study was to develop a zein/potato starch (PS) film based on chitosan nanoparticles incorporated with curcumin (CCN).
32679329	1	24	theme	zein/potato	177:187	arg1	starch					189:194	zein/potato starch	177:194	a zein/potato starch (PS) film based on chitosan nanoparticles incorporated with curcumin (CCN)	175:269	The aim of this study was to develop a zein/potato starch (PS) film based on chitosan nanoparticles incorporated with curcumin (CCN).
32679329	7	25	theme	prenati	1067:1073	arg1	fillets					1075:1081	Schizothorax prenati fillets	1054:1081	Schizothorax prenati fillets	1054:1081	Further, we evaluated the preservative efficacy of the composite film on Schizothorax prenati fillets by measuring pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS), hardness, microbial counts, organoleptic characteristics, and other fillet quality parameters.
32679329	3	26	theme	%	521:521	arg1	%					528:528	84.8% ± 1.1%	517:528	84.8% ± 1.1%	517:528	Our results showed that the CCN encapsulated effectively curcumin (CUR) (84.8% ± 1.1%) and presented with high oxidation resistance and relative release efficiency.
32679329	3	26	theme	%	521:521	arg1	curcumin					501:508	curcumin	501:508	curcumin (CUR) (84.8% ± 1.1%)	501:529	Our results showed that the CCN encapsulated effectively curcumin (CUR) (84.8% ± 1.1%) and presented with high oxidation resistance and relative release efficiency.
32679329	1	27	theme	starch	189:194	arg1	film					201:204	a zein/potato starch (PS) film	175:204	a zein/potato starch (PS) film based on chitosan nanoparticles incorporated with curcumin (CCN)	175:269	The aim of this study was to develop a zein/potato starch (PS) film based on chitosan nanoparticles incorporated with curcumin (CCN).
32679329	9	28	theme	Schizothorax	1555:1566	arg1	fillets					1576:1582	Schizothorax prenati fillets	1555:1582	Schizothorax prenati fillets	1555:1582	In conclusion, our work shows that CCN/zein/PS composite film holds promise as a potential bioactive packaging material for Schizothorax prenati fillets.
32679329	5	29	theme	oxygen	726:731	arg1	OP					747:748	OP	747:748	OP	747:748	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	29	theme	oxygen	726:731	arg1	properties					714:723	the mechanical properties	699:723	the mechanical properties	699:723	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	29	theme	oxygen	726:731	arg1	permeability					733:744	oxygen permeability	726:744	oxygen permeability (OP)	726:749	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	30	theme	water	752:756	arg1	permeability					764:775	water vapor permeability	752:775	water vapor permeability (WVP)	752:781	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	30	theme	water	752:756	arg1	properties					714:723	the mechanical properties	699:723	the mechanical properties	699:723	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	30	theme	water	752:756	arg1	WVP					778:780	WVP	778:780	WVP	778:780	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	2	31	theme	relative	392:399	arg1	release					401:407	relative release	392:407	relative release	392:407	The CCN film was characterized for encapsulation efficiency, particle size, zeta potential, polydispersity index (PDI), relative release, and DPPH radical scavenging test.
32679329	3	32	theme	relative	580:587	arg1	efficiency					597:606	relative release efficiency	580:606	relative release efficiency	580:606	Our results showed that the CCN encapsulated effectively curcumin (CUR) (84.8% ± 1.1%) and presented with high oxidation resistance and relative release efficiency.
32679329	2	33	theme	CCN	276:278	arg1	film					280:283	The CCN film	272:283	The CCN film	272:283	The CCN film was characterized for encapsulation efficiency, particle size, zeta potential, polydispersity index (PDI), relative release, and DPPH radical scavenging test.
32679329	5	34	theme	release	793:799	arg1	properties					714:723	the mechanical properties	699:723	the mechanical properties	699:723	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	34	theme	release	793:799	arg1	efficiency					801:810	relative release efficiency	784:810	relative release efficiency	784:810	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	7	35	theme	Schizothorax	1054:1065	arg1	fillets					1075:1081	Schizothorax prenati fillets	1054:1081	Schizothorax prenati fillets	1054:1081	Further, we evaluated the preservative efficacy of the composite film on Schizothorax prenati fillets by measuring pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS), hardness, microbial counts, organoleptic characteristics, and other fillet quality parameters.
32679329	3	36	theme	release	589:595	arg1	efficiency					597:606	relative release efficiency	580:606	relative release efficiency	580:606	Our results showed that the CCN encapsulated effectively curcumin (CUR) (84.8% ± 1.1%) and presented with high oxidation resistance and relative release efficiency.
32679329	5	37	theme	relative	784:791	arg1	properties					714:723	the mechanical properties	699:723	the mechanical properties	699:723	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	37	theme	relative	784:791	arg1	efficiency					801:810	relative release efficiency	784:810	relative release efficiency	784:810	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	7	38	theme	film	1046:1049	arg1	efficacy					1020:1027	the preservative efficacy	1003:1027	the preservative efficacy of the composite film	1003:1049	Further, we evaluated the preservative efficacy of the composite film on Schizothorax prenati fillets by measuring pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS), hardness, microbial counts, organoleptic characteristics, and other fillet quality parameters.
32679329	0	39	theme	based	47:51	arg1	zein					53:56	based zein	47:56	based zein	47:56	Preparation of chitosan/curcumin nanoparticles based zein and potato starch composite films for Schizothorax prenati fillet preservation.
32679329	2	40	theme	scavenging	427:436	arg1	test					438:441	DPPH radical scavenging test	414:441	DPPH radical scavenging test	414:441	The CCN film was characterized for encapsulation efficiency, particle size, zeta potential, polydispersity index (PDI), relative release, and DPPH radical scavenging test.
32679329	6	41	theme	composite	922:930	arg1	film					932:935	the composite film	918:935	the composite film	918:935	We observed that the composite film had good mechanical and barrier properties.
32679329	7	42	theme	total	1100:1104	arg1	TVB-N					1131:1135	TVB-N	1131:1135	TVB-N	1131:1135	Further, we evaluated the preservative efficacy of the composite film on Schizothorax prenati fillets by measuring pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS), hardness, microbial counts, organoleptic characteristics, and other fillet quality parameters.
32679329	7	42	theme	total	1100:1104	arg1	nitrogen					1121:1128	total volatile basic nitrogen	1100:1128	total volatile basic nitrogen (TVB-N)	1100:1136	Further, we evaluated the preservative efficacy of the composite film on Schizothorax prenati fillets by measuring pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS), hardness, microbial counts, organoleptic characteristics, and other fillet quality parameters.
32679329	2	43	theme	radical	419:425	arg1	test					438:441	DPPH radical scavenging test	414:441	DPPH radical scavenging test	414:441	The CCN film was characterized for encapsulation efficiency, particle size, zeta potential, polydispersity index (PDI), relative release, and DPPH radical scavenging test.
32679329	7	44	theme	volatile	1106:1113	arg1	TVB-N					1131:1135	TVB-N	1131:1135	TVB-N	1131:1135	Further, we evaluated the preservative efficacy of the composite film on Schizothorax prenati fillets by measuring pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS), hardness, microbial counts, organoleptic characteristics, and other fillet quality parameters.
32679329	7	44	theme	volatile	1106:1113	arg1	nitrogen					1121:1128	total volatile basic nitrogen	1100:1128	total volatile basic nitrogen (TVB-N)	1100:1136	Further, we evaluated the preservative efficacy of the composite film on Schizothorax prenati fillets by measuring pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS), hardness, microbial counts, organoleptic characteristics, and other fillet quality parameters.
32679329	0	45	theme	starch	69:74	arg1	films					86:90	potato starch composite films	62:90	potato starch composite films	62:90	Preparation of chitosan/curcumin nanoparticles based zein and potato starch composite films for Schizothorax prenati fillet preservation.
32679329	8	46	theme	physicochemical	1322:1336	arg1	changes					1338:1344	physicochemical changes	1322:1344	physicochemical changes in the Schizothorax prenati fillets	1322:1380	The CCN/zein/PS composite film delayed physicochemical changes in the Schizothorax prenati fillets and prolonged their shelf life by up to 15 days.
32679329	5	47	theme	DPPH	817:820	arg1	properties					714:723	the mechanical properties	699:723	the mechanical properties	699:723	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	47	theme	DPPH	817:820	arg1	properties					841:850	DPPH radical scavenging properties	817:850	DPPH radical scavenging properties	817:850	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	2	48	theme	DPPH	414:417	arg1	test					438:441	DPPH radical scavenging test	414:441	DPPH radical scavenging test	414:441	The CCN film was characterized for encapsulation efficiency, particle size, zeta potential, polydispersity index (PDI), relative release, and DPPH radical scavenging test.
32679329	7	49	theme	organoleptic	1216:1227	arg1	characteristics					1229:1243	organoleptic characteristics	1216:1243	organoleptic characteristics	1216:1243	Further, we evaluated the preservative efficacy of the composite film on Schizothorax prenati fillets by measuring pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS), hardness, microbial counts, organoleptic characteristics, and other fillet quality parameters.
32679329	8	50	theme	shelf	1402:1406	arg1	life					1408:1411	their shelf life	1396:1411	their shelf life	1396:1411	The CCN/zein/PS composite film delayed physicochemical changes in the Schizothorax prenati fillets and prolonged their shelf life by up to 15 days.
32679329	9	51	theme	bioactive	1522:1530	arg1	material					1542:1549	a potential bioactive packaging material	1510:1549	a potential bioactive packaging material for Schizothorax prenati fillets	1510:1582	In conclusion, our work shows that CCN/zein/PS composite film holds promise as a potential bioactive packaging material for Schizothorax prenati fillets.
32679329	9	51	theme	bioactive	1522:1530	arg1	promise					1499:1505	promise	1499:1505	promise	1499:1505	In conclusion, our work shows that CCN/zein/PS composite film holds promise as a potential bioactive packaging material for Schizothorax prenati fillets.
32679329	5	52	theme	radical	822:828	arg1	properties					714:723	the mechanical properties	699:723	the mechanical properties	699:723	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	52	theme	radical	822:828	arg1	properties					841:850	DPPH radical scavenging properties	817:850	DPPH radical scavenging properties	817:850	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	7	53	theme	other	1250:1254	arg1	parameters					1271:1280	other fillet quality parameters	1250:1280	other fillet quality parameters	1250:1280	Further, we evaluated the preservative efficacy of the composite film on Schizothorax prenati fillets by measuring pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS), hardness, microbial counts, organoleptic characteristics, and other fillet quality parameters.
32679329	7	54	theme	fillet	1256:1261	arg1	parameters					1271:1280	other fillet quality parameters	1250:1280	other fillet quality parameters	1250:1280	Further, we evaluated the preservative efficacy of the composite film on Schizothorax prenati fillets by measuring pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS), hardness, microbial counts, organoleptic characteristics, and other fillet quality parameters.
32679329	3	55	theme	high	550:553	arg1	resistance					565:574	high oxidation resistance	550:574	high oxidation resistance	550:574	Our results showed that the CCN encapsulated effectively curcumin (CUR) (84.8% ± 1.1%) and presented with high oxidation resistance and relative release efficiency.
32679329	8	56	theme	composite	1299:1307	arg1	film					1309:1312	The CCN/zein/PS composite film	1283:1312	The CCN/zein/PS composite film	1283:1312	The CCN/zein/PS composite film delayed physicochemical changes in the Schizothorax prenati fillets and prolonged their shelf life by up to 15 days.
32679329	2	57	theme	encapsulation	307:319	arg1	efficiency					321:330	encapsulation efficiency	307:330	encapsulation efficiency	307:330	The CCN film was characterized for encapsulation efficiency, particle size, zeta potential, polydispersity index (PDI), relative release, and DPPH radical scavenging test.
32679329	5	58	theme	composite	859:867	arg1	films					869:873	the composite films	855:873	the composite films of different mass ratios	855:898	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	6	59	contain	had	937:939	arg1	film					932:935	the composite film	918:935	the composite film	918:935	We observed that the composite film had good mechanical and barrier properties.
32679329	6	59	contain	had	937:939	arg2	properties					969:978	good mechanical and barrier properties	941:978	properties	969:978	We observed that the composite film had good mechanical and barrier properties.
32679329	6	59	contain	had	937:939	arg2	mechanical					946:955	mechanical	946:955	mechanical	946:955	We observed that the composite film had good mechanical and barrier properties.
32679329	3	60	theme	oxidation	555:563	arg1	resistance					565:574	high oxidation resistance	550:574	high oxidation resistance	550:574	Our results showed that the CCN encapsulated effectively curcumin (CUR) (84.8% ± 1.1%) and presented with high oxidation resistance and relative release efficiency.
32679329	4	61	theme	composite	625:633	arg1	round					646:650	round	646:650	round	646:650	The CCN/zein/PS composite films were round, smooth, and compact.
32679329	4	61	theme	composite	625:633	arg1	films					635:639	The CCN/zein/PS composite films	609:639	The CCN/zein/PS composite films	609:639	The CCN/zein/PS composite films were round, smooth, and compact.
32679329	5	62	theme	scavenging	830:839	arg1	properties					714:723	the mechanical properties	699:723	the mechanical properties	699:723	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	5	62	theme	scavenging	830:839	arg1	properties					841:850	DPPH radical scavenging properties	817:850	DPPH radical scavenging properties	817:850	We measured and compared the mechanical properties, oxygen permeability (OP), water vapor permeability (WVP), relative release efficiency, and DPPH radical scavenging properties of the composite films of different mass ratios.
32679329	0	63	theme	composite	76:84	arg1	films					86:90	potato starch composite films	62:90	potato starch composite films	62:90	Preparation of chitosan/curcumin nanoparticles based zein and potato starch composite films for Schizothorax prenati fillet preservation.
32679329	8	64	theme	CCN/zein/PS	1287:1297	arg1	film					1309:1312	The CCN/zein/PS composite film	1283:1312	The CCN/zein/PS composite film	1283:1312	The CCN/zein/PS composite film delayed physicochemical changes in the Schizothorax prenati fillets and prolonged their shelf life by up to 15 days.
32679329	8	65	theme	Schizothorax	1353:1364	arg1	fillets					1374:1380	the Schizothorax prenati fillets	1349:1380	the Schizothorax prenati fillets	1349:1380	The CCN/zein/PS composite film delayed physicochemical changes in the Schizothorax prenati fillets and prolonged their shelf life by up to 15 days.
32679329	6	66	theme	barrier	961:967	arg1	properties					969:978	good mechanical and barrier properties	941:978	properties	969:978	We observed that the composite film had good mechanical and barrier properties.
32679329	2	67	theme	zeta	348:351	arg1	potential					353:361	zeta potential	348:361	zeta potential	348:361	The CCN film was characterized for encapsulation efficiency, particle size, zeta potential, polydispersity index (PDI), relative release, and DPPH radical scavenging test.
32679329	7	68	theme	microbial	1198:1206	arg1	counts					1208:1213	microbial counts	1198:1213	microbial counts	1198:1213	Further, we evaluated the preservative efficacy of the composite film on Schizothorax prenati fillets by measuring pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS), hardness, microbial counts, organoleptic characteristics, and other fillet quality parameters.
32679329	1	69	theme	chitosan	215:222	arg1	nanoparticles					224:236	chitosan nanoparticles	215:236	chitosan nanoparticles incorporated with curcumin (CCN)	215:269	The aim of this study was to develop a zein/potato starch (PS) film based on chitosan nanoparticles incorporated with curcumin (CCN).
32679329	0	70	theme	potato	62:67	arg1	films					86:90	potato starch composite films	62:90	potato starch composite films	62:90	Preparation of chitosan/curcumin nanoparticles based zein and potato starch composite films for Schizothorax prenati fillet preservation.
32679329	0	71	theme	chitosan/curcumin	15:31	arg1	Preparation					0:10	Preparation	0:10	Preparation of chitosan/curcumin	0:31	Preparation of chitosan/curcumin nanoparticles based zein and potato starch composite films for Schizothorax prenati fillet preservation.
32679329	1	72	theme	study	154:158	arg1	aim					142:144	The aim	138:144	The aim of this study	138:158	The aim of this study was to develop a zein/potato starch (PS) film based on chitosan nanoparticles incorporated with curcumin (CCN).
32679329	7	73	theme	basic	1115:1119	arg1	TVB-N					1131:1135	TVB-N	1131:1135	TVB-N	1131:1135	Further, we evaluated the preservative efficacy of the composite film on Schizothorax prenati fillets by measuring pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS), hardness, microbial counts, organoleptic characteristics, and other fillet quality parameters.
32679329	7	73	theme	basic	1115:1119	arg1	nitrogen					1121:1128	total volatile basic nitrogen	1100:1128	total volatile basic nitrogen (TVB-N)	1100:1136	Further, we evaluated the preservative efficacy of the composite film on Schizothorax prenati fillets by measuring pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBARS), hardness, microbial counts, organoleptic characteristics, and other fillet quality parameters.
34420758	3	0	theme	reduced	580:586	arg1	properties					496:505	properties	496:505	properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin	496:704	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	0	theme	reduced	580:586	arg1	uptake					594:599	reduced water uptake	580:599	reduced water uptake (45%)	580:605	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	0	theme	reduced	580:586	arg1	%					604:604	45%	602:604	45%	602:604	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	4	1	theme	thermomechanical	834:849	arg1	processing					851:860	high-viscosity thermomechanical processing	819:860	high-viscosity thermomechanical processing	819:860	We attribute this synergy to the ionic and hydrogen-bonding interactions between the two biopolymers enabled by high-viscosity thermomechanical processing.
34420758	3	2	theme	Young	515:519	arg1	603.8 MPa					532:540	603.8 MPa	532:540	603.8 MPa	532:540	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	2	theme	Young	515:519	arg1	modulus					523:529	higher Young's modulus	508:529	higher Young's modulus (603.8 MPa)	508:541	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	2	theme	Young	515:519	arg1	properties					496:505	properties	496:505	properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin	496:704	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	4	3	theme	high-viscosity	819:832	arg1	processing					851:860	high-viscosity thermomechanical processing	819:860	high-viscosity thermomechanical processing	819:860	We attribute this synergy to the ionic and hydrogen-bonding interactions between the two biopolymers enabled by high-viscosity thermomechanical processing.
34420758	5	4	theme	lowest	875:880	arg1	hygroscopicity					882:895	the lowest hygroscopicity	871:895	the lowest hygroscopicity	871:895	Despite the lowest hygroscopicity, the 1:1 chitosan:gelatin films displayed the highest surface hydrophilicity.
34420758	7	5	theme	e.g.	1171:1174	arg1	packaging					1176:1184	e.g. packaging	1171:1184	e.g. packaging	1171:1184	Thus, this work has generated new understanding to enhance the application of biopolymers for e.g. packaging, coating, and biomedical applications.
34420758	3	6	dep	properties	496:505	arg1	33.6 MPa					565:572	33.6 MPa	565:572	33.6 MPa	565:572	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	6	dep	properties	496:505	arg1	603.8 MPa					532:540	603.8 MPa	532:540	603.8 MPa	532:540	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	6	dep	properties	496:505	arg1	modulus					523:529	higher Young's modulus	508:529	higher Young's modulus (603.8 MPa)	508:541	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	6	dep	properties	496:505	arg1	properties					496:505	properties	496:505	properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin	496:704	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	6	dep	properties	496:505	arg1	strength					555:562	tensile strength	547:562	tensile strength (33.6 MPa)	547:573	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	7	theme	tensile	547:553	arg1	33.6 MPa					565:572	33.6 MPa	565:572	33.6 MPa	565:572	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	7	theme	tensile	547:553	arg1	properties					496:505	properties	496:505	properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin	496:704	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	7	theme	tensile	547:553	arg1	strength					555:562	tensile strength	547:562	tensile strength (33.6 MPa)	547:573	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	5	8	theme	chitosan	906:913	arg1	films					923:927	the 1:1 chitosan:gelatin films	898:927	the 1:1 chitosan:gelatin films	898:927	Despite the lowest hygroscopicity, the 1:1 chitosan:gelatin films displayed the highest surface hydrophilicity.
34420758	7	9	theme	biopolymers	1155:1165	arg1	application					1140:1150	the application	1136:1150	the application of biopolymers for e.g. packaging, coating, and biomedical applications	1136:1222	Thus, this work has generated new understanding to enhance the application of biopolymers for e.g. packaging, coating, and biomedical applications.
34420758	5	10	theme	surface	951:957	arg1	hydrophilicity					959:972	the highest surface hydrophilicity	939:972	the highest surface hydrophilicity	939:972	Despite the lowest hygroscopicity, the 1:1 chitosan:gelatin films displayed the highest surface hydrophilicity.
34420758	1	11	theme	environmentally	185:199	arg1	materials					234:242	environmentally benign, bio-safe, and functional materials	185:242	environmentally benign, bio-safe, and functional materials	185:242	Chitosan and gelatin are attractive polymeric feedstocks for developing environmentally benign, bio-safe, and functional materials.
34420758	2	12	theme	materials	301:309	arg1	properties					311:320	advantageous materials properties	288:320	advantageous materials properties	288:320	However, cost-effective methods to achieve advantageous materials properties and tailor their functionality are still lacking, but interesting.
34420758	2	13	dep	methods	269:275	arg1	tailor					326:331	tailor	326:331	tailor their functionality	326:351	However, cost-effective methods to achieve advantageous materials properties and tailor their functionality are still lacking, but interesting.
34420758	2	13	dep	methods	269:275	arg1	achieve					280:286	achieve	280:286	achieve advantageous materials properties	280:320	However, cost-effective methods to achieve advantageous materials properties and tailor their functionality are still lacking, but interesting.
34420758	0	14	theme	processed	19:27	arg1	films					46:50	Thermomechanically processed chitosan:gelatin films	0:50	Thermomechanically processed chitosan:gelatin films	0:50	Thermomechanically processed chitosan:gelatin films being transparent, mechanically robust and less hygroscopic.
34420758	3	15	theme	1:1	453:455	arg1	w/w					458:460	w/w	458:460	w/w	458:460	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	15	theme	1:1	453:455	arg1	ratio					463:467	1:1 (w/w) ratio	453:467	1:1 (w/w) ratio	453:467	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	16	theme	mixing	422:427	arg1	chitosan					429:436	chitosan	429:436	chitosan	429:436	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	5	17	theme	gelatin	915:921	arg1	films					923:927	the 1:1 chitosan:gelatin films	898:927	the 1:1 chitosan:gelatin films	898:927	Despite the lowest hygroscopicity, the 1:1 chitosan:gelatin films displayed the highest surface hydrophilicity.
34420758	1	18	theme	bio-safe	209:216	arg1	materials					234:242	environmentally benign, bio-safe, and functional materials	185:242	environmentally benign, bio-safe, and functional materials	185:242	Chitosan and gelatin are attractive polymeric feedstocks for developing environmentally benign, bio-safe, and functional materials.
34420758	2	19	theme	cost-effective	254:267	arg1	methods					269:275	cost-effective methods	254:275	cost-effective methods to achieve advantageous materials properties and tailor their functionality	254:351	However, cost-effective methods to achieve advantageous materials properties and tailor their functionality are still lacking, but interesting.
34420758	0	20	theme	chitosan	29:36	arg1	films					46:50	Thermomechanically processed chitosan:gelatin films	0:50	Thermomechanically processed chitosan:gelatin films	0:50	Thermomechanically processed chitosan:gelatin films being transparent, mechanically robust and less hygroscopic.
34420758	0	21	dep	transparent	58:68	arg1	robust					84:89	robust	84:89	robust	84:89	Thermomechanically processed chitosan:gelatin films being transparent, mechanically robust and less hygroscopic.
34420758	1	22	theme	functional	223:232	arg1	materials					234:242	environmentally benign, bio-safe, and functional materials	185:242	environmentally benign, bio-safe, and functional materials	185:242	Chitosan and gelatin are attractive polymeric feedstocks for developing environmentally benign, bio-safe, and functional materials.
34420758	0	23	theme	gelatin	38:44	arg1	films					46:50	Thermomechanically processed chitosan:gelatin films	0:50	Thermomechanically processed chitosan:gelatin films	0:50	Thermomechanically processed chitosan:gelatin films being transparent, mechanically robust and less hygroscopic.
34420758	3	24	with	materials	481:489	arg1	modulus					523:529	higher Young's modulus	508:529	higher Young's modulus (603.8 MPa)	508:541	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	24	with	materials	481:489	arg1	uptake					594:599	reduced water uptake	580:599	reduced water uptake (45%)	580:605	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	24	with	materials	481:489	arg1	properties					496:505	properties	496:505	properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin	496:704	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	24	with	materials	481:489	arg1	strength					555:562	tensile strength	547:562	tensile strength (33.6 MPa)	547:573	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	25	theme	water	588:592	arg1	properties					496:505	properties	496:505	properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin	496:704	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	25	theme	water	588:592	arg1	uptake					594:599	reduced water uptake	580:599	reduced water uptake (45%)	580:605	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	25	theme	water	588:592	arg1	%					604:604	45%	602:604	45%	602:604	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	26	from	ratio	463:467	arg1	gelatin					442:448	gelatin	442:448	gelatin	442:448	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	26	from	ratio	463:467	arg1	chitosan					429:436	chitosan	429:436	chitosan	429:436	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	5	27	theme	highest	943:949	arg1	hydrophilicity					959:972	the highest surface hydrophilicity	939:972	the highest surface hydrophilicity	939:972	Despite the lowest hygroscopicity, the 1:1 chitosan:gelatin films displayed the highest surface hydrophilicity.
34420758	7	28	theme	new	1107:1109	arg1	understanding					1111:1123	new understanding	1107:1123	new understanding	1107:1123	Thus, this work has generated new understanding to enhance the application of biopolymers for e.g. packaging, coating, and biomedical applications.
34420758	3	29	theme	higher	508:513	arg1	603.8 MPa					532:540	603.8 MPa	532:540	603.8 MPa	532:540	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	29	theme	higher	508:513	arg1	modulus					523:529	higher Young's modulus	508:529	higher Young's modulus (603.8 MPa)	508:541	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	29	theme	higher	508:513	arg1	properties					496:505	properties	496:505	properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin	496:704	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	30	theme	better	635:640	arg1	modulus					523:529	higher Young's modulus	508:529	higher Young's modulus (603.8 MPa)	508:541	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	30	theme	better	635:640	arg1	uptake					594:599	reduced water uptake	580:599	reduced water uptake (45%)	580:605	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	30	theme	better	635:640	arg1	properties					496:505	properties	496:505	properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin	496:704	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	3	30	theme	better	635:640	arg1	strength					555:562	tensile strength	547:562	tensile strength (33.6 MPa)	547:573	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	2	31	theme	advantageous	288:299	arg1	properties					311:320	advantageous materials properties	288:320	advantageous materials properties	288:320	However, cost-effective methods to achieve advantageous materials properties and tailor their functionality are still lacking, but interesting.
34420758	4	32	theme	hydrogen-bonding	750:765	arg1	interactions					767:778	the ionic and hydrogen-bonding interactions	736:778	the ionic and hydrogen-bonding interactions between the two biopolymers enabled by high-viscosity thermomechanical processing	736:860	We attribute this synergy to the ionic and hydrogen-bonding interactions between the two biopolymers enabled by high-viscosity thermomechanical processing.
34420758	5	33	theme	1:1	902:904	arg1	films					923:927	the 1:1 chitosan:gelatin films	898:927	the 1:1 chitosan:gelatin films	898:927	Despite the lowest hygroscopicity, the 1:1 chitosan:gelatin films displayed the highest surface hydrophilicity.
34420758	3	34	theme	water	620:624	arg1	soaking					626:632	water soaking	620:632	water soaking	620:632	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	1	35	theme	attractive	138:147	arg1	feedstocks					159:168	attractive polymeric feedstocks	138:168	attractive polymeric feedstocks for developing environmentally benign, bio-safe, and functional materials	138:242	Chitosan and gelatin are attractive polymeric feedstocks for developing environmentally benign, bio-safe, and functional materials.
34420758	1	35	theme	attractive	138:147	arg1	gelatin					126:132	gelatin	126:132	gelatin	126:132	Chitosan and gelatin are attractive polymeric feedstocks for developing environmentally benign, bio-safe, and functional materials.
34420758	1	35	theme	attractive	138:147	arg1	Chitosan					113:120	Chitosan	113:120	Chitosan	113:120	Chitosan and gelatin are attractive polymeric feedstocks for developing environmentally benign, bio-safe, and functional materials.
34420758	3	36	theme	soaking	626:632	arg1	6 h					613:615	6 h	613:615	6 h of water soaking	613:632	Herein, we found that physically mixing chitosan and gelatin at 1:1 (w/w) ratio resulted in materials with properties (higher Young's modulus (603.8 MPa) and tensile strength (33.6 MPa), and reduced water uptake (45%) after 6 h of water soaking) better than those of the materials based on mainly chitosan or gelatin.
34420758	1	37	theme	polymeric	149:157	arg1	feedstocks					159:168	attractive polymeric feedstocks	138:168	attractive polymeric feedstocks for developing environmentally benign, bio-safe, and functional materials	138:242	Chitosan and gelatin are attractive polymeric feedstocks for developing environmentally benign, bio-safe, and functional materials.
34420758	1	37	theme	polymeric	149:157	arg1	gelatin					126:132	gelatin	126:132	gelatin	126:132	Chitosan and gelatin are attractive polymeric feedstocks for developing environmentally benign, bio-safe, and functional materials.
34420758	1	37	theme	polymeric	149:157	arg1	Chitosan					113:120	Chitosan	113:120	Chitosan	113:120	Chitosan and gelatin are attractive polymeric feedstocks for developing environmentally benign, bio-safe, and functional materials.
34420758	1	38	theme	benign	201:206	arg1	materials					234:242	environmentally benign, bio-safe, and functional materials	185:242	environmentally benign, bio-safe, and functional materials	185:242	Chitosan and gelatin are attractive polymeric feedstocks for developing environmentally benign, bio-safe, and functional materials.
34420758	6	39	theme	gelatin	996:1002	arg1	addition					984:991	addition	984:991	addition of gelatin to chitosan	984:1014	Besides, addition of gelatin to chitosan led to films being brighter, more transparent and amorphous.
34420758	4	40	theme	ionic	740:744	arg1	interactions					767:778	the ionic and hydrogen-bonding interactions	736:778	the ionic and hydrogen-bonding interactions between the two biopolymers enabled by high-viscosity thermomechanical processing	736:860	We attribute this synergy to the ionic and hydrogen-bonding interactions between the two biopolymers enabled by high-viscosity thermomechanical processing.
34420758	7	41	theme	biomedical	1200:1209	arg1	applications					1211:1222	biomedical applications	1200:1222	biomedical applications	1200:1222	Thus, this work has generated new understanding to enhance the application of biopolymers for e.g. packaging, coating, and biomedical applications.
34560151	2	0	theme	algae	373:377	arg1	tertiolecta					407:417	tertiolecta	407:417	tertiolecta	407:417	The presented work primarily focused on exploring the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL).
34560151	2	0	theme	algae	373:377	arg1	residue					387:393	marine de-oiled green algae biomass residue	351:393	marine de-oiled green algae biomass residue (Dunaliella tertiolecta)	351:418	The presented work primarily focused on exploring the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL).
34560151	6	1	theme	increased	896:904	arg1	modulus					906:912	increased modulus	896:912	increased modulus of 18.38 MPa and tensile strength	896:946	Besides, increased modulus of 18.38 MPa and tensile strength was obtained in PCL/CNC/1 bio-composites.
34560151	1	2	theme	biodegradable	162:174	arg1	materials					176:184	biodegradable materials	162:184	biodegradable materials	162:184	Exploration of biodegradable materials for conventional application has taken a rising interest across the world.
34560151	3	3	link	derived	513:519	arg1	CNCs					521:524	The washed algae biomass residue (WABR) and algae derived CNCs	463:524	The washed algae biomass residue (WABR) and algae derived CNCs	463:524	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	3	3	link	derived	513:519	arg1	bio-fillers					557:567	two different bio-fillers	543:567	two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading	543:697	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	7	4	from	reduction	1053:1061	arg1	degree					1070:1075	the degree	1066:1075	the degree of crystallinity of bio-composites	1066:1110	However, the isothermal kinetics study (at 45 °C) revealed the reduction in the degree of crystallinity of bio-composites, and the axialite formation was visualized via POM.
34560151	0	5	theme	crystallization	82:96	arg1	kinetics					98:105	crystallization kinetics	82:105	crystallization kinetics of poly(ɛ-caprolactone) bio-composites	82:144	Utilization of microalgae residue and isolated cellulose nanocrystals: A study on crystallization kinetics of poly(ɛ-caprolactone) bio-composites.
34560151	7	6	theme	bio-composites	1097:1110	arg1	crystallinity					1080:1092	crystallinity	1080:1092	crystallinity of bio-composites	1080:1110	However, the isothermal kinetics study (at 45 °C) revealed the reduction in the degree of crystallinity of bio-composites, and the axialite formation was visualized via POM.
34560151	3	7	theme	biomass	480:486	arg1	residue					488:494	The washed algae biomass residue	463:494	The washed algae biomass residue (WABR)	463:501	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	3	7	theme	biomass	480:486	arg1	WABR					497:500	WABR	497:500	WABR	497:500	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	0	8	theme	poly	110:113	arg1	bio-composites					131:144	poly(ɛ-caprolactone) bio-composites	110:144	poly(ɛ-caprolactone) bio-composites	110:144	Utilization of microalgae residue and isolated cellulose nanocrystals: A study on crystallization kinetics of poly(ɛ-caprolactone) bio-composites.
34560151	3	9	dep	derived	513:519	arg1	residue					488:494	The washed algae biomass residue	463:494	The washed algae biomass residue (WABR)	463:501	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	3	9	dep	derived	513:519	arg1	WABR					497:500	WABR	497:500	WABR	497:500	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	3	9	dep	derived	513:519	arg1	algae					507:511	algae	507:511	algae	507:511	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	3	10	theme	derived	513:519	arg1	CNCs					521:524	The washed algae biomass residue (WABR) and algae derived CNCs	463:524	The washed algae biomass residue (WABR) and algae derived CNCs	463:524	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	3	10	theme	derived	513:519	arg1	bio-fillers					557:567	two different bio-fillers	543:567	two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading	543:697	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	8	11	theme	effective	1226:1234	arg1	CNCs					1174:1177	CNCs	1174:1177	CNCs	1174:1177	Moreover, CNCs was found as an excellent nucleating agent and effective bio-filler as compared to WABR.
34560151	8	11	theme	effective	1226:1234	arg1	bio-filler					1236:1245	effective bio-filler	1226:1245	effective bio-filler	1226:1245	Moreover, CNCs was found as an excellent nucleating agent and effective bio-filler as compared to WABR.
34560151	1	12	theme	materials	176:184	arg1	Exploration					147:157	Exploration	147:157	Exploration of biodegradable materials for conventional application	147:213	Exploration of biodegradable materials for conventional application has taken a rising interest across the world.
34560151	7	13	theme	axialite	1121:1128	arg1	formation					1130:1138	the axialite formation	1117:1138	the axialite formation	1117:1138	However, the isothermal kinetics study (at 45 °C) revealed the reduction in the degree of crystallinity of bio-composites, and the axialite formation was visualized via POM.
34560151	7	14	theme	crystallinity	1080:1092	arg1	degree					1070:1075	the degree	1066:1075	the degree of crystallinity of bio-composites	1066:1110	However, the isothermal kinetics study (at 45 °C) revealed the reduction in the degree of crystallinity of bio-composites, and the axialite formation was visualized via POM.
34560151	5	15	theme	PCL/CNC	848:854	arg1	bio-composites					856:869	PCL/CNC bio-composites	848:869	PCL/CNC bio-composites	848:869	Improved thermal stability was observed in PCL/CNC bio-composites by ~10 °C rise.
34560151	2	16	theme	isolated	332:339	arg1	CNCs					341:344	isolated CNCs	332:344	isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta)	332:418	The presented work primarily focused on exploring the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL).
34560151	1	17	theme	conventional	190:201	arg1	application					203:213	conventional application	190:213	conventional application	190:213	Exploration of biodegradable materials for conventional application has taken a rising interest across the world.
34560151	6	18	theme	18.38 MPa	917:925	arg1	modulus					906:912	increased modulus	896:912	increased modulus of 18.38 MPa and tensile strength	896:946	Besides, increased modulus of 18.38 MPa and tensile strength was obtained in PCL/CNC/1 bio-composites.
34560151	2	19	from	residue	387:393	arg1	effectiveness					315:327	the effectiveness	311:327	the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL)	311:460	The presented work primarily focused on exploring the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL).
34560151	2	19	from	residue	387:393	arg1	CNCs					341:344	isolated CNCs	332:344	isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta)	332:418	The presented work primarily focused on exploring the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL).
34560151	3	20	theme	bio-composites	652:665	arg1	development					610:620	development	610:620	development	610:620	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	3	20	theme	bio-composites	652:665	arg1	comparison					595:604	comparison	595:604	comparison	595:604	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	0	21	theme	residue	26:32	arg1	Utilization					0:10	Utilization	0:10	Utilization of microalgae residue and isolated cellulose nanocrystals: A study on crystallization kinetics of poly(ɛ-caprolactone) bio-composites.	0:145	Utilization of microalgae residue and isolated cellulose nanocrystals: A study on crystallization kinetics of poly(ɛ-caprolactone) bio-composites.
34560151	5	22	theme	thermal	814:820	arg1	stability					822:830	Improved thermal stability	805:830	Improved thermal stability	805:830	Improved thermal stability was observed in PCL/CNC bio-composites by ~10 °C rise.
34560151	0	23	theme	microalgae	15:24	arg1	residue					26:32	microalgae residue	15:32	microalgae residue	15:32	Utilization of microalgae residue and isolated cellulose nanocrystals: A study on crystallization kinetics of poly(ɛ-caprolactone) bio-composites.
34560151	3	24	theme	flexible	643:650	arg1	bio-composites					652:665	biodegradable and flexible bio-composites	625:665	biodegradable and flexible bio-composites with varying bio-filler loading	625:697	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	2	25	theme	green	367:371	arg1	algae					373:377	marine de-oiled green algae	351:377	marine de-oiled green algae biomass residue (Dunaliella tertiolecta)	351:418	The presented work primarily focused on exploring the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL).
34560151	3	26	theme	washed	467:472	arg1	residue					488:494	The washed algae biomass residue	463:494	The washed algae biomass residue (WABR)	463:501	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	3	26	theme	washed	467:472	arg1	WABR					497:500	WABR	497:500	WABR	497:500	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	0	27	theme	isolated	38:45	arg1	nanocrystals					57:68	isolated cellulose nanocrystals	38:68	isolated cellulose nanocrystals	38:68	Utilization of microalgae residue and isolated cellulose nanocrystals: A study on crystallization kinetics of poly(ɛ-caprolactone) bio-composites.
34560151	0	28	theme	bio-composites	131:144	arg1	kinetics					98:105	crystallization kinetics	82:105	crystallization kinetics of poly(ɛ-caprolactone) bio-composites	82:144	Utilization of microalgae residue and isolated cellulose nanocrystals: A study on crystallization kinetics of poly(ɛ-caprolactone) bio-composites.
34560151	0	29	from	study	73:77	arg1	kinetics					98:105	crystallization kinetics	82:105	crystallization kinetics of poly(ɛ-caprolactone) bio-composites	82:144	Utilization of microalgae residue and isolated cellulose nanocrystals: A study on crystallization kinetics of poly(ɛ-caprolactone) bio-composites.
34560151	4	30	theme	PCL/WABR	768:775	arg1	bio-composites					789:802	the developed PCL/WABR and PCL/CNC bio-composites	754:802	the developed PCL/WABR and PCL/CNC bio-composites	754:802	FTIR, XRD, TGA, UTM, DSC, POM, and SAXS characterized the developed PCL/WABR and PCL/CNC bio-composites.
34560151	3	31	with	bio-composites	652:665	arg1	loading					691:697	varying bio-filler loading	672:697	varying bio-filler loading	672:697	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	2	32	theme	synthesized	423:433	arg1	ɛ-caprolactone					440:453	ɛ-caprolactone	440:453	ɛ-caprolactone	440:453	The presented work primarily focused on exploring the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL).
34560151	2	32	theme	synthesized	423:433	arg1	PCL					457:459	PCL	457:459	PCL	457:459	The presented work primarily focused on exploring the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL).
34560151	2	32	theme	synthesized	423:433	arg1	poly					435:438	synthesized poly	423:438	synthesized poly(ɛ-caprolactone) (PCL)	423:460	The presented work primarily focused on exploring the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL).
34560151	6	33	theme	strength	939:946	arg1	modulus					906:912	increased modulus	896:912	increased modulus of 18.38 MPa and tensile strength	896:946	Besides, increased modulus of 18.38 MPa and tensile strength was obtained in PCL/CNC/1 bio-composites.
34560151	3	34	theme	biodegradable	625:637	arg1	bio-composites					652:665	biodegradable and flexible bio-composites	625:665	biodegradable and flexible bio-composites with varying bio-filler loading	625:697	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	4	35	theme	PCL/CNC	781:787	arg1	bio-composites					789:802	the developed PCL/WABR and PCL/CNC bio-composites	754:802	the developed PCL/WABR and PCL/CNC bio-composites	754:802	FTIR, XRD, TGA, UTM, DSC, POM, and SAXS characterized the developed PCL/WABR and PCL/CNC bio-composites.
34560151	7	36	theme	kinetics	1014:1021	arg1	study					1023:1027	the isothermal kinetics study	999:1027	the isothermal kinetics study (at 45 °C)	999:1038	However, the isothermal kinetics study (at 45 °C) revealed the reduction in the degree of crystallinity of bio-composites, and the axialite formation was visualized via POM.
34560151	1	37	theme	rising	227:232	arg1	interest					234:241	a rising interest	225:241	a rising interest across the world	225:258	Exploration of biodegradable materials for conventional application has taken a rising interest across the world.
34560151	0	38	theme	nanocrystals	57:68	arg1	Utilization					0:10	Utilization	0:10	Utilization of microalgae residue and isolated cellulose nanocrystals: A study on crystallization kinetics of poly(ɛ-caprolactone) bio-composites.	0:145	Utilization of microalgae residue and isolated cellulose nanocrystals: A study on crystallization kinetics of poly(ɛ-caprolactone) bio-composites.
34560151	6	39	theme	tensile	931:937	arg1	strength					939:946	tensile strength	931:946	tensile strength	931:946	Besides, increased modulus of 18.38 MPa and tensile strength was obtained in PCL/CNC/1 bio-composites.
34560151	5	40	theme	Improved	805:812	arg1	stability					822:830	Improved thermal stability	805:830	Improved thermal stability	805:830	Improved thermal stability was observed in PCL/CNC bio-composites by ~10 °C rise.
34560151	2	41	theme	de-oiled	358:365	arg1	algae					373:377	marine de-oiled green algae	351:377	marine de-oiled green algae biomass residue (Dunaliella tertiolecta)	351:418	The presented work primarily focused on exploring the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL).
34560151	0	42	theme	cellulose	47:55	arg1	nanocrystals					57:68	isolated cellulose nanocrystals	38:68	isolated cellulose nanocrystals	38:68	Utilization of microalgae residue and isolated cellulose nanocrystals: A study on crystallization kinetics of poly(ɛ-caprolactone) bio-composites.
34560151	3	43	theme	bio-filler	680:689	arg1	loading					691:697	varying bio-filler loading	672:697	varying bio-filler loading	672:697	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	2	44	theme	marine	351:356	arg1	algae					373:377	marine de-oiled green algae	351:377	marine de-oiled green algae biomass residue (Dunaliella tertiolecta)	351:418	The presented work primarily focused on exploring the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL).
34560151	3	45	theme	different	547:555	arg1	CNCs					521:524	The washed algae biomass residue (WABR) and algae derived CNCs	463:524	The washed algae biomass residue (WABR) and algae derived CNCs	463:524	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	3	45	theme	different	547:555	arg1	bio-fillers					557:567	two different bio-fillers	543:567	two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading	543:697	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	4	46	theme	developed	758:766	arg1	bio-composites					789:802	the developed PCL/WABR and PCL/CNC bio-composites	754:802	the developed PCL/WABR and PCL/CNC bio-composites	754:802	FTIR, XRD, TGA, UTM, DSC, POM, and SAXS characterized the developed PCL/WABR and PCL/CNC bio-composites.
34560151	7	47	theme	isothermal	1003:1012	arg1	study					1023:1027	the isothermal kinetics study	999:1027	the isothermal kinetics study (at 45 °C)	999:1038	However, the isothermal kinetics study (at 45 °C) revealed the reduction in the degree of crystallinity of bio-composites, and the axialite formation was visualized via POM.
34560151	3	48	theme	algae	474:478	arg1	residue					488:494	The washed algae biomass residue	463:494	The washed algae biomass residue (WABR)	463:501	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	3	48	theme	algae	474:478	arg1	WABR					497:500	WABR	497:500	WABR	497:500	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	5	49	located	observed	836:843	arg1	bio-composites					856:869	PCL/CNC bio-composites	848:869	PCL/CNC bio-composites	848:869	Improved thermal stability was observed in PCL/CNC bio-composites by ~10 °C rise.
34560151	5	49	located	observed	836:843	arg2	stability					822:830	Improved thermal stability	805:830	Improved thermal stability	805:830	Improved thermal stability was observed in PCL/CNC bio-composites by ~10 °C rise.
34560151	2	50	theme	presented	265:273	arg1	work					275:278	The presented work	261:278	The presented work	261:278	The presented work primarily focused on exploring the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL).
34560151	2	51	theme	CNCs	341:344	arg1	effectiveness					315:327	the effectiveness	311:327	the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL)	311:460	The presented work primarily focused on exploring the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL).
34560151	3	52	theme	varying	672:678	arg1	loading					691:697	varying bio-filler loading	672:697	varying bio-filler loading	672:697	The washed algae biomass residue (WABR) and algae derived CNCs were explored as two different bio-fillers incorporated into PCL for comparison and development of biodegradable and flexible bio-composites with varying bio-filler loading.
34560151	8	53	theme	nucleating	1205:1214	arg1	CNCs					1174:1177	CNCs	1174:1177	CNCs	1174:1177	Moreover, CNCs was found as an excellent nucleating agent and effective bio-filler as compared to WABR.
34560151	8	53	theme	nucleating	1205:1214	arg1	agent					1216:1220	an excellent nucleating agent	1192:1220	an excellent nucleating agent	1192:1220	Moreover, CNCs was found as an excellent nucleating agent and effective bio-filler as compared to WABR.
34560151	7	54	from	45 °C	1033:1037	arg1	study					1023:1027	the isothermal kinetics study	999:1027	the isothermal kinetics study (at 45 °C)	999:1038	However, the isothermal kinetics study (at 45 °C) revealed the reduction in the degree of crystallinity of bio-composites, and the axialite formation was visualized via POM.
34560151	8	55	theme	excellent	1195:1203	arg1	CNCs					1174:1177	CNCs	1174:1177	CNCs	1174:1177	Moreover, CNCs was found as an excellent nucleating agent and effective bio-filler as compared to WABR.
34560151	8	55	theme	excellent	1195:1203	arg1	agent					1216:1220	an excellent nucleating agent	1192:1220	an excellent nucleating agent	1192:1220	Moreover, CNCs was found as an excellent nucleating agent and effective bio-filler as compared to WABR.
34560151	0	56	dep	Utilization	0:10	arg1	study					73:77	A study	71:77	Utilization of microalgae residue and isolated cellulose nanocrystals: A study on crystallization kinetics of poly(ɛ-caprolactone) bio-composites.	0:145	Utilization of microalgae residue and isolated cellulose nanocrystals: A study on crystallization kinetics of poly(ɛ-caprolactone) bio-composites.
34560151	2	57	theme	biomass	379:385	arg1	tertiolecta					407:417	tertiolecta	407:417	tertiolecta	407:417	The presented work primarily focused on exploring the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL).
34560151	2	57	theme	biomass	379:385	arg1	residue					387:393	marine de-oiled green algae biomass residue	351:393	marine de-oiled green algae biomass residue (Dunaliella tertiolecta)	351:418	The presented work primarily focused on exploring the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL).
34560151	2	58	from	effectiveness	315:327	arg1	tertiolecta					407:417	tertiolecta	407:417	tertiolecta	407:417	The presented work primarily focused on exploring the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL).
34560151	2	58	from	effectiveness	315:327	arg1	ɛ-caprolactone					440:453	ɛ-caprolactone	440:453	ɛ-caprolactone	440:453	The presented work primarily focused on exploring the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL).
34560151	2	58	from	effectiveness	315:327	arg1	PCL					457:459	PCL	457:459	PCL	457:459	The presented work primarily focused on exploring the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL).
34560151	2	58	from	effectiveness	315:327	arg1	poly					435:438	synthesized poly	423:438	synthesized poly(ɛ-caprolactone) (PCL)	423:460	The presented work primarily focused on exploring the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL).
34560151	2	58	from	effectiveness	315:327	arg1	residue					387:393	marine de-oiled green algae biomass residue	351:393	marine de-oiled green algae biomass residue (Dunaliella tertiolecta)	351:418	The presented work primarily focused on exploring the effectiveness of isolated CNCs from marine de-oiled green algae biomass residue (Dunaliella tertiolecta) in synthesized poly(ɛ-caprolactone) (PCL).
33887307	0	0	theme	Acids	101:105	arg1	Identification					0:13	Identification	0:13	Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure.	0:156	Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure.
33887307	1	1	theme	acid	351:354	arg1	composition					286:296	composition	286:296	composition of microbiota	286:310	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	1	1	theme	acid	351:354	arg1	implications					376:387	clinical implications	367:387	clinical implications of these findings	367:405	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	1	1	theme	acid	351:354	arg1	levels					313:318	levels	313:318	levels of endotoxins and lipoteichoic acid (LTA)	313:360	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	13	2	theme	higher	1769:1774	arg1	levels					1776:1781	higher levels	1769:1781	higher levels of LTA	1769:1788	In addition, higher levels of LTA were statistically associated with larger periapical radiolucent areas, but no correlation between this feature and LPS was found.
33887307	1	3	from	presence	179:186	arg1	canal					224:228	the root canal	215:228	the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings	215:405	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	10	4	theme	Significant	1421:1431	arg1	correlations					1445:1456	Significant statistical correlations	1421:1456	Significant statistical correlations	1421:1456	Significant statistical correlations were found between levels of LTA and clinical features.
33887307	1	5	theme	secondary/persistent	244:263	arg1	infection					265:273	secondary/persistent infection	244:273	secondary/persistent infection	244:273	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	0	6	theme	Lipoteichoic	88:99	arg1	Acids					101:105	Lipoteichoic Acids	88:105	Lipoteichoic Acids	88:105	Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure.
33887307	11	7	theme	species	1651:1657	arg1	species					1651:1657	species	1651:1657	species	1651:1657	CONCLUSION Despite the prevalence of gram-positives, the microbiota present in secondary/persistent infections showed a large variety of species.
33887307	11	7	theme	species	1651:1657	arg1	variety					1640:1646	a large variety	1632:1646	a large variety of species	1632:1657	CONCLUSION Despite the prevalence of gram-positives, the microbiota present in secondary/persistent infections showed a large variety of species.
33887307	10	8	theme	statistical	1433:1443	arg1	correlations					1445:1456	Significant statistical correlations	1421:1456	Significant statistical correlations	1421:1456	Significant statistical correlations were found between levels of LTA and clinical features.
33887307	2	9	theme	endodontic	481:490	arg1	retreatment					492:502	endodontic retreatment	481:502	endodontic retreatment	481:502	METHOD Samples were collected from root canals of 50 patients who needed endodontic retreatment and had radiographic evidence of apical periodontitis.
33887307	10	10	theme	LTA	1487:1489	arg1	levels					1477:1482	levels	1477:1482	levels of LTA and clinical features	1477:1511	Significant statistical correlations were found between levels of LTA and clinical features.
33887307	0	11	theme	Root	112:115	arg1	Canals					117:122	Root Canals	112:122	Root Canals of Teeth With Endodontic Failure	112:155	Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure.
33887307	0	12	from	Identification	0:13	arg1	Canals					117:122	Root Canals	112:122	Root Canals of Teeth With Endodontic Failure	112:155	Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure.
33887307	5	13	link	enzyme-linked	848:860	arg1	assay					876:880	enzyme-linked immunosorbent assay	848:880	enzyme-linked immunosorbent assay	848:880	Lipopolysaccharides (LPS) and LTAs were quantified by using, respectively, limulus amebocyte lysate and enzyme-linked immunosorbent assay tests.
33887307	5	13	link	enzyme-linked	848:860	arg1	tests					882:886	, respectively, limulus amebocyte lysate and enzyme-linked immunosorbent assay tests	803:886	tests	882:886	Lipopolysaccharides (LPS) and LTAs were quantified by using, respectively, limulus amebocyte lysate and enzyme-linked immunosorbent assay tests.
33887307	7	14	theme	strains	998:1004	arg1	total					971:975	A total	969:975	A total	969:975	A total of 154 gram-positive strains, of 188 strains isolated, were found in the root canals by culture.
33887307	13	15	theme	radiolucent	1843:1853	arg1	areas					1855:1859	larger periapical radiolucent areas	1825:1859	larger periapical radiolucent areas	1825:1859	In addition, higher levels of LTA were statistically associated with larger periapical radiolucent areas, but no correlation between this feature and LPS was found.
33887307	11	16	theme	large	1634:1638	arg1	species					1651:1657	species	1651:1657	species	1651:1657	CONCLUSION Despite the prevalence of gram-positives, the microbiota present in secondary/persistent infections showed a large variety of species.
33887307	11	16	theme	large	1634:1638	arg1	variety					1640:1646	a large variety	1632:1646	a large variety of species	1632:1657	CONCLUSION Despite the prevalence of gram-positives, the microbiota present in secondary/persistent infections showed a large variety of species.
33887307	8	17	theme	biochemical	1170:1180	arg1	tests					1182:1186	the biochemical tests	1166:1186	the biochemical tests	1166:1186	Enterococcus faecalis and Gemella morbillorum were the most prevalent species identified by the biochemical tests, whereas molecular analyses (nested-PCR) showed a high frequency of P. gingivalis, E. faecalis, and Fusobacterium nucleatum.
33887307	2	18	theme	radiographic	512:523	arg1	evidence					525:532	radiographic evidence	512:532	radiographic evidence of apical periodontitis	512:556	METHOD Samples were collected from root canals of 50 patients who needed endodontic retreatment and had radiographic evidence of apical periodontitis.
33887307	8	19	theme	Fusobacterium	1288:1300	arg1	nucleatum					1302:1310	Fusobacterium nucleatum	1288:1310	Fusobacterium nucleatum	1288:1310	Enterococcus faecalis and Gemella morbillorum were the most prevalent species identified by the biochemical tests, whereas molecular analyses (nested-PCR) showed a high frequency of P. gingivalis, E. faecalis, and Fusobacterium nucleatum.
33887307	5	20	theme	immunosorbent	862:874	arg1	assay					876:880	enzyme-linked immunosorbent assay	848:880	enzyme-linked immunosorbent assay	848:880	Lipopolysaccharides (LPS) and LTAs were quantified by using, respectively, limulus amebocyte lysate and enzyme-linked immunosorbent assay tests.
33887307	5	20	theme	immunosorbent	862:874	arg1	tests					882:886	, respectively, limulus amebocyte lysate and enzyme-linked immunosorbent assay tests	803:886	tests	882:886	Lipopolysaccharides (LPS) and LTAs were quantified by using, respectively, limulus amebocyte lysate and enzyme-linked immunosorbent assay tests.
33887307	8	21	theme	gingivalis	1259:1268	arg1	nucleatum					1302:1310	Fusobacterium nucleatum	1288:1310	Fusobacterium nucleatum	1288:1310	Enterococcus faecalis and Gemella morbillorum were the most prevalent species identified by the biochemical tests, whereas molecular analyses (nested-PCR) showed a high frequency of P. gingivalis, E. faecalis, and Fusobacterium nucleatum.
33887307	8	21	theme	gingivalis	1259:1268	arg1	frequency					1243:1251	a high frequency	1236:1251	a high frequency of P. gingivalis	1236:1268	Enterococcus faecalis and Gemella morbillorum were the most prevalent species identified by the biochemical tests, whereas molecular analyses (nested-PCR) showed a high frequency of P. gingivalis, E. faecalis, and Fusobacterium nucleatum.
33887307	13	22	theme	larger	1825:1830	arg1	areas					1855:1859	larger periapical radiolucent areas	1825:1859	larger periapical radiolucent areas	1825:1859	In addition, higher levels of LTA were statistically associated with larger periapical radiolucent areas, but no correlation between this feature and LPS was found.
33887307	11	23	theme	microbiota	1571:1580	arg1	present					1582:1588	the microbiota present	1567:1588	the microbiota present in secondary/persistent infections	1567:1623	CONCLUSION Despite the prevalence of gram-positives, the microbiota present in secondary/persistent infections showed a large variety of species.
33887307	5	24	theme	amebocyte	827:835	arg1	tests					882:886	, respectively, limulus amebocyte lysate and enzyme-linked immunosorbent assay tests	803:886	tests	882:886	Lipopolysaccharides (LPS) and LTAs were quantified by using, respectively, limulus amebocyte lysate and enzyme-linked immunosorbent assay tests.
33887307	5	24	theme	amebocyte	827:835	arg1	lysate					837:842	limulus amebocyte lysate	819:842	limulus amebocyte lysate	819:842	Lipopolysaccharides (LPS) and LTAs were quantified by using, respectively, limulus amebocyte lysate and enzyme-linked immunosorbent assay tests.
33887307	9	25	theme	mean	1360:1363	arg1	values					1365:1370	mean values	1360:1370	mean values	1360:1370	LPS and LTA were detected in all samples, with mean values being 3.52 EU/mL and 597.83 pg/mL, respectively.
33887307	2	26	theme	patients	461:468	arg1	canals					448:453	root canals	443:453	root canals of 50 patients who needed endodontic retreatment and had radiographic evidence of apical periodontitis	443:556	METHOD Samples were collected from root canals of 50 patients who needed endodontic retreatment and had radiographic evidence of apical periodontitis.
33887307	1	27	theme	clinical	367:374	arg1	implications					376:387	clinical implications	367:387	clinical implications of these findings	367:405	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	11	28	theme	secondary/persistent	1593:1612	arg1	infections					1614:1623	secondary/persistent infections	1593:1623	secondary/persistent infections	1593:1623	CONCLUSION Despite the prevalence of gram-positives, the microbiota present in secondary/persistent infections showed a large variety of species.
33887307	6	29	located	detected	911:918	arg1	samples					927:933	all samples	923:933	all samples	923:933	RESULTS Bacteria were detected in all samples by culture and molecular methods.
33887307	6	29	located	detected	911:918	arg2	Bacteria					897:904	RESULTS Bacteria	889:904	RESULTS Bacteria	889:904	RESULTS Bacteria were detected in all samples by culture and molecular methods.
33887307	2	30	theme	root	443:446	arg1	canals					448:453	root canals	443:453	root canals of 50 patients who needed endodontic retreatment and had radiographic evidence of apical periodontitis	443:556	METHOD Samples were collected from root canals of 50 patients who needed endodontic retreatment and had radiographic evidence of apical periodontitis.
33887307	8	31	dep	species	1144:1150	arg1	whereas					1189:1195	whereas	1189:1195	whereas	1189:1195	Enterococcus faecalis and Gemella morbillorum were the most prevalent species identified by the biochemical tests, whereas molecular analyses (nested-PCR) showed a high frequency of P. gingivalis, E. faecalis, and Fusobacterium nucleatum.
33887307	8	32	theme	molecular	1197:1205	arg1	nested-PCR					1217:1226	nested-PCR	1217:1226	nested-PCR	1217:1226	Enterococcus faecalis and Gemella morbillorum were the most prevalent species identified by the biochemical tests, whereas molecular analyses (nested-PCR) showed a high frequency of P. gingivalis, E. faecalis, and Fusobacterium nucleatum.
33887307	8	32	theme	molecular	1197:1205	arg1	analyses					1207:1214	molecular analyses	1197:1214	molecular analyses (nested-PCR)	1197:1227	Enterococcus faecalis and Gemella morbillorum were the most prevalent species identified by the biochemical tests, whereas molecular analyses (nested-PCR) showed a high frequency of P. gingivalis, E. faecalis, and Fusobacterium nucleatum.
33887307	1	33	theme	apical	191:196	arg1	periodontitis					198:210	apical periodontitis	191:210	apical periodontitis	191:210	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	13	34	theme	LTA	1786:1788	arg1	levels					1776:1781	higher levels	1769:1781	higher levels of LTA	1769:1788	In addition, higher levels of LTA were statistically associated with larger periapical radiolucent areas, but no correlation between this feature and LPS was found.
33887307	11	35	dep	CONCLUSION	1514:1523	arg1	prevalence					1537:1546	the prevalence	1533:1546	the prevalence of gram-positives	1533:1564	CONCLUSION Despite the prevalence of gram-positives, the microbiota present in secondary/persistent infections showed a large variety of species.
33887307	10	36	theme	clinical	1495:1502	arg1	features					1504:1511	clinical features	1495:1511	clinical features	1495:1511	Significant statistical correlations were found between levels of LTA and clinical features.
33887307	7	37	theme	gram-positive	984:996	arg1	strains					998:1004	154 gram-positive strains	980:1004	154 gram-positive strains	980:1004	A total of 154 gram-positive strains, of 188 strains isolated, were found in the root canals by culture.
33887307	1	38	theme	periodontitis	198:210	arg1	presence					179:186	the presence	175:186	the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings	175:405	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	0	39	theme	Culturable	18:27	arg1	Microorganisms					47:60	Microorganisms	47:60	Microorganisms	47:60	Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure.
33887307	1	40	theme	microbiota	301:310	arg1	composition					286:296	composition	286:296	composition of microbiota	286:310	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	1	40	theme	microbiota	301:310	arg1	implications					376:387	clinical implications	367:387	clinical implications of these findings	367:405	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	1	40	theme	microbiota	301:310	arg1	levels					313:318	levels	313:318	levels of endotoxins and lipoteichoic acid (LTA)	313:360	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	0	41	theme	Teeth	127:131	arg1	Canals					117:122	Root Canals	112:122	Root Canals of Teeth With Endodontic Failure	112:155	Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure.
33887307	1	42	attach	presence	179:186	arg1	canal					224:228	the root canal	215:228	the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings	215:405	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	1	42	attach	presence	179:186	arg2	periodontitis					198:210	apical periodontitis	191:210	apical periodontitis	191:210	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	4	43	theme	chain	662:666	arg1	nested-PCR					678:687	nested-PCR	678:687	nested-PCR	678:687	Nested-polymerase chain reaction (nested-PCR) was used to identify 17 species of specific bacteria.
33887307	4	43	theme	chain	662:666	arg1	reaction					668:675	Nested-polymerase chain reaction	644:675	Nested-polymerase chain reaction (nested-PCR)	644:688	Nested-polymerase chain reaction (nested-PCR) was used to identify 17 species of specific bacteria.
33887307	0	44	theme	Microorganisms	47:60	arg1	Identification					0:13	Identification	0:13	Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure.	0:156	Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure.
33887307	2	45	theme	periodontitis	544:556	arg1	evidence					525:532	radiographic evidence	512:532	radiographic evidence of apical periodontitis	512:556	METHOD Samples were collected from root canals of 50 patients who needed endodontic retreatment and had radiographic evidence of apical periodontitis.
33887307	8	46	theme	P.	1256:1257	arg1	gingivalis					1259:1268	P. gingivalis	1256:1268	P. gingivalis	1256:1268	Enterococcus faecalis and Gemella morbillorum were the most prevalent species identified by the biochemical tests, whereas molecular analyses (nested-PCR) showed a high frequency of P. gingivalis, E. faecalis, and Fusobacterium nucleatum.
33887307	2	47	theme	METHOD	408:413	arg1	Samples					415:421	METHOD Samples	408:421	METHOD Samples	408:421	METHOD Samples were collected from root canals of 50 patients who needed endodontic retreatment and had radiographic evidence of apical periodontitis.
33887307	0	48	theme	Nonculturable	33:45	arg1	Microorganisms					47:60	Microorganisms	47:60	Microorganisms	47:60	Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure.
33887307	0	49	theme	Endodontic	138:147	arg1	Failure					149:155	Endodontic Failure	138:155	Endodontic Failure	138:155	Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure.
33887307	6	50	theme	RESULTS	889:895	arg1	Bacteria					897:904	RESULTS Bacteria	889:904	RESULTS Bacteria	889:904	RESULTS Bacteria were detected in all samples by culture and molecular methods.
33887307	4	51	theme	Nested-polymerase	644:660	arg1	nested-PCR					678:687	nested-PCR	678:687	nested-PCR	678:687	Nested-polymerase chain reaction (nested-PCR) was used to identify 17 species of specific bacteria.
33887307	4	51	theme	Nested-polymerase	644:660	arg1	reaction					668:675	Nested-polymerase chain reaction	644:675	Nested-polymerase chain reaction (nested-PCR)	644:688	Nested-polymerase chain reaction (nested-PCR) was used to identify 17 species of specific bacteria.
33887307	4	52	used	used	694:697	arg2	nested-PCR					678:687	nested-PCR	678:687	nested-PCR	678:687	Nested-polymerase chain reaction (nested-PCR) was used to identify 17 species of specific bacteria.
33887307	4	52	used	used	694:697	arg2	reaction					668:675	Nested-polymerase chain reaction	644:675	Nested-polymerase chain reaction (nested-PCR)	644:688	Nested-polymerase chain reaction (nested-PCR) was used to identify 17 species of specific bacteria.
33887307	1	53	theme	root	219:222	arg1	canal					224:228	the root canal	215:228	the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings	215:405	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	0	54	theme	Lipopolysaccharides	63:81	arg1	Identification					0:13	Identification	0:13	Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure.	0:156	Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure.
33887307	7	55	located	found	1037:1041	arg1	canals					1055:1060	the root canals	1046:1060	the root canals	1046:1060	A total of 154 gram-positive strains, of 188 strains isolated, were found in the root canals by culture.
33887307	7	55	located	found	1037:1041	arg2	total					971:975	A total	969:975	A total	969:975	A total of 154 gram-positive strains, of 188 strains isolated, were found in the root canals by culture.
33887307	7	56	theme	root	1050:1053	arg1	canals					1055:1060	the root canals	1046:1060	the root canals	1046:1060	A total of 154 gram-positive strains, of 188 strains isolated, were found in the root canals by culture.
33887307	1	57	theme	findings	398:405	arg1	composition					286:296	composition	286:296	composition of microbiota	286:310	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	1	57	theme	findings	398:405	arg1	implications					376:387	clinical implications	367:387	clinical implications of these findings	367:405	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	1	57	theme	findings	398:405	arg1	levels					313:318	levels	313:318	levels of endotoxins and lipoteichoic acid (LTA)	313:360	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	9	58	located	detected	1330:1337	arg2	LTA					1321:1323	LTA	1321:1323	LTA	1321:1323	LPS and LTA were detected in all samples, with mean values being 3.52 EU/mL and 597.83 pg/mL, respectively.
33887307	9	58	located	detected	1330:1337	arg1	samples					1346:1352	all samples	1342:1352	all samples	1342:1352	LPS and LTA were detected in all samples, with mean values being 3.52 EU/mL and 597.83 pg/mL, respectively.
33887307	9	58	located	detected	1330:1337	arg2	LPS					1313:1315	LPS	1313:1315	LPS	1313:1315	LPS and LTA were detected in all samples, with mean values being 3.52 EU/mL and 597.83 pg/mL, respectively.
33887307	4	59	theme	bacteria	734:741	arg1	species					714:720	17 species	711:720	17 species of specific bacteria	711:741	Nested-polymerase chain reaction (nested-PCR) was used to identify 17 species of specific bacteria.
33887307	2	60	theme	apical	537:542	arg1	periodontitis					544:556	apical periodontitis	537:556	apical periodontitis	537:556	METHOD Samples were collected from root canals of 50 patients who needed endodontic retreatment and had radiographic evidence of apical periodontitis.
33887307	1	61	theme	endotoxins	323:332	arg1	composition					286:296	composition	286:296	composition of microbiota	286:310	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	1	61	theme	endotoxins	323:332	arg1	implications					376:387	clinical implications	367:387	clinical implications of these findings	367:405	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	1	61	theme	endotoxins	323:332	arg1	levels					313:318	levels	313:318	levels of endotoxins and lipoteichoic acid (LTA)	313:360	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	6	62	theme	molecular	950:958	arg1	methods					960:966	molecular methods	950:966	molecular methods	950:966	RESULTS Bacteria were detected in all samples by culture and molecular methods.
33887307	12	63	located	found	1701:1705	arg2	associations					1683:1694	associations	1683:1694	associations	1683:1694	Within this diversity, associations were found between specific bacteria and clinical features.
33887307	12	63	located	found	1701:1705	arg1	diversity					1672:1680	this diversity	1667:1680	this diversity	1667:1680	Within this diversity, associations were found between specific bacteria and clinical features.
33887307	3	64	theme	culture	603:609	arg1	technique					611:619	the culture technique	599:619	the culture technique	599:619	Microorganisms were identified by using the culture technique and biochemical tests.
33887307	4	65	theme	specific	725:732	arg1	bacteria					734:741	specific bacteria	725:741	specific bacteria	725:741	Nested-polymerase chain reaction (nested-PCR) was used to identify 17 species of specific bacteria.
33887307	12	66	theme	clinical	1737:1744	arg1	features					1746:1753	clinical features	1737:1753	clinical features	1737:1753	Within this diversity, associations were found between specific bacteria and clinical features.
33887307	7	67	theme	strains	1014:1020	arg1	total					971:975	A total	969:975	A total	969:975	A total of 154 gram-positive strains, of 188 strains isolated, were found in the root canals by culture.
33887307	8	68	theme	prevalent	1134:1142	arg1	species					1144:1150	the most prevalent species	1125:1150	the most prevalent species identified by the biochemical tests	1125:1186	Enterococcus faecalis and Gemella morbillorum were the most prevalent species identified by the biochemical tests, whereas molecular analyses (nested-PCR) showed a high frequency of P. gingivalis, E. faecalis, and Fusobacterium nucleatum.
33887307	8	68	theme	prevalent	1134:1142	arg1	morbillorum					1108:1118	Gemella morbillorum	1100:1118	Gemella morbillorum	1100:1118	Enterococcus faecalis and Gemella morbillorum were the most prevalent species identified by the biochemical tests, whereas molecular analyses (nested-PCR) showed a high frequency of P. gingivalis, E. faecalis, and Fusobacterium nucleatum.
33887307	8	68	theme	prevalent	1134:1142	arg1	faecalis					1087:1094	Enterococcus faecalis	1074:1094	Enterococcus faecalis	1074:1094	Enterococcus faecalis and Gemella morbillorum were the most prevalent species identified by the biochemical tests, whereas molecular analyses (nested-PCR) showed a high frequency of P. gingivalis, E. faecalis, and Fusobacterium nucleatum.
33887307	0	69	from	Canals	117:122	arg1	Microorganisms					47:60	Microorganisms	47:60	Microorganisms	47:60	Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure.
33887307	0	69	from	Canals	117:122	arg1	Identification					0:13	Identification	0:13	Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure.	0:156	Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure.
33887307	0	69	from	Canals	117:122	arg1	Acids					101:105	Lipoteichoic Acids	88:105	Lipoteichoic Acids	88:105	Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure.
33887307	0	69	from	Canals	117:122	arg1	Lipopolysaccharides					63:81	Lipopolysaccharides	63:81	Lipopolysaccharides	63:81	Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure.
33887307	11	70	theme	gram-positives	1551:1564	arg1	prevalence					1537:1546	the prevalence	1533:1546	the prevalence of gram-positives	1533:1564	CONCLUSION Despite the prevalence of gram-positives, the microbiota present in secondary/persistent infections showed a large variety of species.
33887307	10	71	theme	features	1504:1511	arg1	levels					1477:1482	levels	1477:1482	levels of LTA and clinical features	1477:1511	Significant statistical correlations were found between levels of LTA and clinical features.
33887307	5	72	dep	tests	882:886	arg1	assay					876:880	enzyme-linked immunosorbent assay	848:880	enzyme-linked immunosorbent assay	848:880	Lipopolysaccharides (LPS) and LTAs were quantified by using, respectively, limulus amebocyte lysate and enzyme-linked immunosorbent assay tests.
33887307	5	72	dep	tests	882:886	arg1	tests					882:886	, respectively, limulus amebocyte lysate and enzyme-linked immunosorbent assay tests	803:886	tests	882:886	Lipopolysaccharides (LPS) and LTAs were quantified by using, respectively, limulus amebocyte lysate and enzyme-linked immunosorbent assay tests.
33887307	5	72	dep	tests	882:886	arg1	lysate					837:842	limulus amebocyte lysate	819:842	limulus amebocyte lysate	819:842	Lipopolysaccharides (LPS) and LTAs were quantified by using, respectively, limulus amebocyte lysate and enzyme-linked immunosorbent assay tests.
33887307	2	73	contain	had	508:510	arg2	evidence					525:532	radiographic evidence	512:532	radiographic evidence of apical periodontitis	512:556	METHOD Samples were collected from root canals of 50 patients who needed endodontic retreatment and had radiographic evidence of apical periodontitis.
33887307	2	73	contain	had	508:510	arg1	patients					461:468	50 patients	458:468	50 patients who needed endodontic retreatment and had radiographic evidence of apical periodontitis	458:556	METHOD Samples were collected from root canals of 50 patients who needed endodontic retreatment and had radiographic evidence of apical periodontitis.
33887307	11	74	from	present	1582:1588	arg1	infections					1614:1623	secondary/persistent infections	1593:1623	secondary/persistent infections	1593:1623	CONCLUSION Despite the prevalence of gram-positives, the microbiota present in secondary/persistent infections showed a large variety of species.
33887307	13	75	theme	periapical	1832:1841	arg1	areas					1855:1859	larger periapical radiolucent areas	1825:1859	larger periapical radiolucent areas	1825:1859	In addition, higher levels of LTA were statistically associated with larger periapical radiolucent areas, but no correlation between this feature and LPS was found.
33887307	12	76	theme	specific	1715:1722	arg1	bacteria					1724:1731	specific bacteria	1715:1731	specific bacteria	1715:1731	Within this diversity, associations were found between specific bacteria and clinical features.
33887307	3	77	theme	biochemical	625:635	arg1	tests					637:641	biochemical tests	625:641	biochemical tests	625:641	Microorganisms were identified by using the culture technique and biochemical tests.
33887307	5	78	theme	enzyme-linked	848:860	arg1	assay					876:880	enzyme-linked immunosorbent assay	848:880	enzyme-linked immunosorbent assay	848:880	Lipopolysaccharides (LPS) and LTAs were quantified by using, respectively, limulus amebocyte lysate and enzyme-linked immunosorbent assay tests.
33887307	5	78	theme	enzyme-linked	848:860	arg1	tests					882:886	, respectively, limulus amebocyte lysate and enzyme-linked immunosorbent assay tests	803:886	tests	882:886	Lipopolysaccharides (LPS) and LTAs were quantified by using, respectively, limulus amebocyte lysate and enzyme-linked immunosorbent assay tests.
33887307	0	79	with	Teeth	127:131	arg1	Failure					149:155	Endodontic Failure	138:155	Endodontic Failure	138:155	Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure.
33887307	1	80	theme	teeth	233:237	arg1	canal					224:228	the root canal	215:228	the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings	215:405	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	8	81	theme	high	1238:1241	arg1	frequency					1243:1251	a high frequency	1236:1251	a high frequency of P. gingivalis	1236:1268	Enterococcus faecalis and Gemella morbillorum were the most prevalent species identified by the biochemical tests, whereas molecular analyses (nested-PCR) showed a high frequency of P. gingivalis, E. faecalis, and Fusobacterium nucleatum.
33887307	1	82	theme	lipoteichoic	338:349	arg1	LTA					357:359	LTA	357:359	LTA	357:359	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	1	82	theme	lipoteichoic	338:349	arg1	acid					351:354	lipoteichoic acid	338:354	lipoteichoic acid (LTA)	338:360	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	1	83	with	canal	224:228	arg1	infection					265:273	secondary/persistent infection	244:273	secondary/persistent infection	244:273	AIM To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings.
33887307	5	84	theme	limulus	819:825	arg1	tests					882:886	, respectively, limulus amebocyte lysate and enzyme-linked immunosorbent assay tests	803:886	tests	882:886	Lipopolysaccharides (LPS) and LTAs were quantified by using, respectively, limulus amebocyte lysate and enzyme-linked immunosorbent assay tests.
33887307	5	84	theme	limulus	819:825	arg1	lysate					837:842	limulus amebocyte lysate	819:842	limulus amebocyte lysate	819:842	Lipopolysaccharides (LPS) and LTAs were quantified by using, respectively, limulus amebocyte lysate and enzyme-linked immunosorbent assay tests.
34599989	0	0	theme	pasting	74:80	arg1	properties					82:91	the physicochemical, functional, structural, and pasting properties	25:91	the physicochemical, functional, structural, and pasting properties of banana starches	25:110	Exploring differences in the physicochemical, functional, structural, and pasting properties of banana starches from dessert, cooking, and plantain cultivars (Musa spp.)
34599989	6	1	theme	59.20	791:795	arg1	%					796:796	59.20%	791:796	59.20%	791:796	Total starch (95.86 and 95.60%,) and resistant starch (65.56 and 59.20%) were higher in Saba and Monthan respectively.
34599989	0	2	theme	Musa	159:162	arg1	spp					164:166	Musa spp	159:166	Musa spp.	159:167	Exploring differences in the physicochemical, functional, structural, and pasting properties of banana starches from dessert, cooking, and plantain cultivars (Musa spp.)
34599989	7	3	theme	Flour	845:849	arg1	86.2-90.6					865:873	86.2-90.6	865:873	86.2-90.6	865:873	Flour colour index (86.2-90.6) was higher in banana starches.
34599989	7	3	theme	Flour	845:849	arg1	index					858:862	Flour colour index	845:862	Flour colour index (86.2-90.6)	845:874	Flour colour index (86.2-90.6) was higher in banana starches.
34599989	12	4	theme	non-food	1436:1443	arg1	industries					1445:1454	food and non-food industries	1427:1454	food and non-food industries in terms of using banana starch in various food compositions and other industrial applications	1427:1549	These findings would be useful for food and non-food industries in terms of using banana starch in various food compositions and other industrial applications.
34599989	0	5	from	cultivars	148:156	arg1	differences					10:20	Exploring differences	0:20	Exploring differences in the physicochemical, functional, structural, and pasting properties of banana starches from dessert, cooking, and plantain cultivars (Musa spp.)	0:168	Exploring differences in the physicochemical, functional, structural, and pasting properties of banana starches from dessert, cooking, and plantain cultivars (Musa spp.)
34599989	0	6	from	differences	10:20	arg1	properties					82:91	the physicochemical, functional, structural, and pasting properties	25:91	the physicochemical, functional, structural, and pasting properties of banana starches	25:110	Exploring differences in the physicochemical, functional, structural, and pasting properties of banana starches from dessert, cooking, and plantain cultivars (Musa spp.)
34599989	8	7	dep	Monthan	1024:1030	arg1	81.65 °C					1043:1050	81.65 °C	1043:1050	81.65 °C	1043:1050	Differential scanning calorimetry and rapid viscosity studies confirmed that starches from Saba (87.67 and 85.71 °C) Monthan (85.36 and 81.65 °C) have a higher gelatinization property.
34599989	8	7	dep	Monthan	1024:1030	arg1	85.36					1033:1037	85.36	1033:1037	85.36	1033:1037	Differential scanning calorimetry and rapid viscosity studies confirmed that starches from Saba (87.67 and 85.71 °C) Monthan (85.36 and 81.65 °C) have a higher gelatinization property.
34599989	8	8	theme	higher	1060:1065	arg1	property					1082:1089	a higher gelatinization property	1058:1089	a higher gelatinization property	1058:1089	Differential scanning calorimetry and rapid viscosity studies confirmed that starches from Saba (87.67 and 85.71 °C) Monthan (85.36 and 81.65 °C) have a higher gelatinization property.
34599989	8	9	from	Saba	998:1001	arg1	starches					984:991	starches	984:991	starches from Saba (87.67 and 85.71 °C) Monthan (85.36 and 81.65 °C)	984:1051	Differential scanning calorimetry and rapid viscosity studies confirmed that starches from Saba (87.67 and 85.71 °C) Monthan (85.36 and 81.65 °C) have a higher gelatinization property.
34599989	11	10	theme	starch	1347:1352	arg1	content					1354:1360	resistant starch content	1337:1360	resistant starch content	1337:1360	PCA showed the greater impact of amylose and resistant starch content on the grouping of varieties.
34599989	12	11	theme	industrial	1527:1536	arg1	applications					1538:1549	other industrial applications	1521:1549	other industrial applications	1521:1549	These findings would be useful for food and non-food industries in terms of using banana starch in various food compositions and other industrial applications.
34599989	10	12	with	rate	1259:1262	arg1	index					1285:1289	lesser glycemic index	1269:1289	lesser glycemic index	1269:1289	The in-vitro starch digestibility revealed that Saba starch has a lower hydrolysis rate with lesser glycemic index.
34599989	1	13	theme	new	301:303	arg1	sources					312:318	new starch sources	301:318	new starch sources	301:318	Banana starch, with its nutritional and functional properties, opens up new opportunities for the food industry, which is seeking new starch sources to fulfil rising demand.
34599989	11	14	theme	content	1354:1360	arg1	impact					1315:1320	the greater impact	1303:1320	the greater impact of amylose and resistant starch content on the grouping of varieties	1303:1389	PCA showed the greater impact of amylose and resistant starch content on the grouping of varieties.
34599989	10	15	theme	Saba	1224:1227	arg1	starch					1229:1234	Saba starch	1224:1234	Saba starch	1224:1234	The in-vitro starch digestibility revealed that Saba starch has a lower hydrolysis rate with lesser glycemic index.
34599989	1	16	theme	starch	305:310	arg1	sources					312:318	new starch sources	301:318	new starch sources	301:318	Banana starch, with its nutritional and functional properties, opens up new opportunities for the food industry, which is seeking new starch sources to fulfil rising demand.
34599989	10	17	theme	glycemic	1276:1283	arg1	index					1285:1289	lesser glycemic index	1269:1289	lesser glycemic index	1269:1289	The in-vitro starch digestibility revealed that Saba starch has a lower hydrolysis rate with lesser glycemic index.
34599989	12	18	theme	various	1491:1497	arg1	compositions					1504:1515	various food compositions	1491:1515	various food compositions	1491:1515	These findings would be useful for food and non-food industries in terms of using banana starch in various food compositions and other industrial applications.
34599989	9	19	dep	B	1113:1113	arg1	%					1172:1172	21.19-52.01%	1161:1172	21.19-52.01%	1161:1172	Banana starches were B and C-type with varying crystallinity levels (21.19-52.01%).
34599989	0	20	theme	starches	103:110	arg1	properties					82:91	the physicochemical, functional, structural, and pasting properties	25:91	the physicochemical, functional, structural, and pasting properties of banana starches	25:110	Exploring differences in the physicochemical, functional, structural, and pasting properties of banana starches from dessert, cooking, and plantain cultivars (Musa spp.)
34599989	8	21	theme	Differential	907:918	arg1	calorimetry					929:939	Differential scanning calorimetry	907:939	Differential scanning calorimetry	907:939	Differential scanning calorimetry and rapid viscosity studies confirmed that starches from Saba (87.67 and 85.71 °C) Monthan (85.36 and 81.65 °C) have a higher gelatinization property.
34599989	2	22	attach	isolated	416:423	arg1	cultivars					461:469	cooking cultivars	453:469	cooking cultivars	453:469	Herein, physico-chemical, and functional properties of banana starches isolated from dessert, plantain, and cooking cultivars were investigated.
34599989	2	22	attach	isolated	416:423	arg1	plantain					439:446	plantain	439:446	plantain	439:446	Herein, physico-chemical, and functional properties of banana starches isolated from dessert, plantain, and cooking cultivars were investigated.
34599989	2	22	attach	isolated	416:423	arg2	starches					407:414	banana starches	400:414	banana starches isolated from dessert, plantain, and cooking cultivars	400:469	Herein, physico-chemical, and functional properties of banana starches isolated from dessert, plantain, and cooking cultivars were investigated.
34599989	2	22	attach	isolated	416:423	arg1	dessert					430:436	dessert	430:436	dessert	430:436	Herein, physico-chemical, and functional properties of banana starches isolated from dessert, plantain, and cooking cultivars were investigated.
34599989	4	23	theme	irregular	583:591	arg1	forms					593:597	irregular forms	583:597	irregular forms with granule sizes ranging from 8.9 to 55.09 μm	583:645	Starch granules registered irregular forms with granule sizes ranging from 8.9 to 55.09 μm.
34599989	0	24	theme	banana	96:101	arg1	starches					103:110	banana starches	96:110	banana starches	96:110	Exploring differences in the physicochemical, functional, structural, and pasting properties of banana starches from dessert, cooking, and plantain cultivars (Musa spp.)
34599989	6	25	theme	resistant	763:771	arg1	starch					773:778	resistant starch	763:778	resistant starch (65.56 and 59.20%)	763:797	Total starch (95.86 and 95.60%,) and resistant starch (65.56 and 59.20%) were higher in Saba and Monthan respectively.
34599989	1	26	theme	functional	211:220	arg1	properties					222:231	its nutritional and functional properties	191:231	its nutritional and functional properties	191:231	Banana starch, with its nutritional and functional properties, opens up new opportunities for the food industry, which is seeking new starch sources to fulfil rising demand.
34599989	10	27	theme	lesser	1269:1274	arg1	index					1285:1289	lesser glycemic index	1269:1289	lesser glycemic index	1269:1289	The in-vitro starch digestibility revealed that Saba starch has a lower hydrolysis rate with lesser glycemic index.
34599989	3	28	theme	Starch	490:495	arg1	yield					497:501	Starch yield	490:501	Starch yield	490:501	Starch yield was higher in Popoulu (30.58%) and Monthan (27.82%).
34599989	2	29	theme	banana	400:405	arg1	starches					407:414	banana starches	400:414	banana starches isolated from dessert, plantain, and cooking cultivars	400:469	Herein, physico-chemical, and functional properties of banana starches isolated from dessert, plantain, and cooking cultivars were investigated.
34599989	8	30	theme	calorimetry	929:939	arg1	studies					961:967	Differential scanning calorimetry and rapid viscosity studies	907:967	Differential scanning calorimetry and rapid viscosity studies	907:967	Differential scanning calorimetry and rapid viscosity studies confirmed that starches from Saba (87.67 and 85.71 °C) Monthan (85.36 and 81.65 °C) have a higher gelatinization property.
34599989	8	31	dep	Saba	998:1001	arg1	87.67					1004:1008	87.67	1004:1008	87.67	1004:1008	Differential scanning calorimetry and rapid viscosity studies confirmed that starches from Saba (87.67 and 85.71 °C) Monthan (85.36 and 81.65 °C) have a higher gelatinization property.
34599989	8	31	dep	Saba	998:1001	arg1	Monthan					1024:1030	Monthan	1024:1030	Monthan	1024:1030	Differential scanning calorimetry and rapid viscosity studies confirmed that starches from Saba (87.67 and 85.71 °C) Monthan (85.36 and 81.65 °C) have a higher gelatinization property.
34599989	8	31	dep	Saba	998:1001	arg1	85.71 °C					1014:1021	85.71 °C	1014:1021	85.71 °C	1014:1021	Differential scanning calorimetry and rapid viscosity studies confirmed that starches from Saba (87.67 and 85.71 °C) Monthan (85.36 and 81.65 °C) have a higher gelatinization property.
34599989	12	32	from	starch	1481:1486	arg1	applications					1538:1549	other industrial applications	1521:1549	other industrial applications	1521:1549	These findings would be useful for food and non-food industries in terms of using banana starch in various food compositions and other industrial applications.
34599989	12	32	from	starch	1481:1486	arg1	compositions					1504:1515	various food compositions	1491:1515	various food compositions	1491:1515	These findings would be useful for food and non-food industries in terms of using banana starch in various food compositions and other industrial applications.
34599989	12	33	theme	food	1499:1502	arg1	compositions					1504:1515	various food compositions	1491:1515	various food compositions	1491:1515	These findings would be useful for food and non-food industries in terms of using banana starch in various food compositions and other industrial applications.
34599989	0	34	theme	Exploring	0:8	arg1	differences					10:20	Exploring differences	0:20	Exploring differences in the physicochemical, functional, structural, and pasting properties of banana starches from dessert, cooking, and plantain cultivars (Musa spp.)	0:168	Exploring differences in the physicochemical, functional, structural, and pasting properties of banana starches from dessert, cooking, and plantain cultivars (Musa spp.)
34599989	11	35	theme	varieties	1381:1389	arg1	grouping					1369:1376	the grouping	1365:1376	the grouping of varieties	1365:1389	PCA showed the greater impact of amylose and resistant starch content on the grouping of varieties.
34599989	8	36	theme	scanning	920:927	arg1	calorimetry					929:939	Differential scanning calorimetry	907:939	Differential scanning calorimetry	907:939	Differential scanning calorimetry and rapid viscosity studies confirmed that starches from Saba (87.67 and 85.71 °C) Monthan (85.36 and 81.65 °C) have a higher gelatinization property.
34599989	11	37	theme	greater	1307:1313	arg1	impact					1315:1320	the greater impact	1303:1320	the greater impact of amylose and resistant starch content on the grouping of varieties	1303:1389	PCA showed the greater impact of amylose and resistant starch content on the grouping of varieties.
34599989	1	38	theme	new	243:245	arg1	opportunities					247:259	new opportunities	243:259	new opportunities for the food industry, which is seeking new starch sources to fulfil rising demand	243:342	Banana starch, with its nutritional and functional properties, opens up new opportunities for the food industry, which is seeking new starch sources to fulfil rising demand.
34599989	1	39	with	starch	178:183	arg1	properties					222:231	its nutritional and functional properties	191:231	its nutritional and functional properties	191:231	Banana starch, with its nutritional and functional properties, opens up new opportunities for the food industry, which is seeking new starch sources to fulfil rising demand.
34599989	10	40	theme	hydrolysis	1248:1257	arg1	rate					1259:1262	a lower hydrolysis rate	1240:1262	a lower hydrolysis rate with lesser glycemic index	1240:1289	The in-vitro starch digestibility revealed that Saba starch has a lower hydrolysis rate with lesser glycemic index.
34599989	10	41	theme	lower	1242:1246	arg1	rate					1259:1262	a lower hydrolysis rate	1240:1262	a lower hydrolysis rate with lesser glycemic index	1240:1289	The in-vitro starch digestibility revealed that Saba starch has a lower hydrolysis rate with lesser glycemic index.
34599989	2	42	theme	functional	375:384	arg1	properties					386:395	functional properties	375:395	functional properties	375:395	Herein, physico-chemical, and functional properties of banana starches isolated from dessert, plantain, and cooking cultivars were investigated.
34599989	1	43	theme	rising	330:335	arg1	demand					337:342	rising demand	330:342	rising demand	330:342	Banana starch, with its nutritional and functional properties, opens up new opportunities for the food industry, which is seeking new starch sources to fulfil rising demand.
34599989	6	44	dep	Total	726:730	arg1	starch					732:737	starch	732:737	starch	732:737	Total starch (95.86 and 95.60%,) and resistant starch (65.56 and 59.20%) were higher in Saba and Monthan respectively.
34599989	1	45	theme	Banana	171:176	arg1	starch					178:183	Banana starch	171:183	Banana starch	171:183	Banana starch, with its nutritional and functional properties, opens up new opportunities for the food industry, which is seeking new starch sources to fulfil rising demand.
34599989	12	46	from	industries	1445:1454	arg1	terms					1459:1463	terms	1459:1463	terms of using banana starch in various food compositions and other industrial applications	1459:1549	These findings would be useful for food and non-food industries in terms of using banana starch in various food compositions and other industrial applications.
34599989	4	47	with	forms	593:597	arg1	sizes					612:616	granule sizes	604:616	granule sizes ranging from 8.9 to 55.09 μm	604:645	Starch granules registered irregular forms with granule sizes ranging from 8.9 to 55.09 μm.
34599989	12	48	theme	banana	1474:1479	arg1	starch					1481:1486	banana starch	1474:1486	banana starch in various food compositions and other industrial applications	1474:1549	These findings would be useful for food and non-food industries in terms of using banana starch in various food compositions and other industrial applications.
34599989	0	49	theme	physicochemical	29:43	arg1	properties					82:91	the physicochemical, functional, structural, and pasting properties	25:91	the physicochemical, functional, structural, and pasting properties of banana starches	25:110	Exploring differences in the physicochemical, functional, structural, and pasting properties of banana starches from dessert, cooking, and plantain cultivars (Musa spp.)
34599989	8	50	theme	viscosity	951:959	arg1	studies					961:967	Differential scanning calorimetry and rapid viscosity studies	907:967	Differential scanning calorimetry and rapid viscosity studies	907:967	Differential scanning calorimetry and rapid viscosity studies confirmed that starches from Saba (87.67 and 85.71 °C) Monthan (85.36 and 81.65 °C) have a higher gelatinization property.
34599989	9	51	theme	Banana	1092:1097	arg1	starches					1099:1106	Banana starches	1092:1106	Banana starches	1092:1106	Banana starches were B and C-type with varying crystallinity levels (21.19-52.01%).
34599989	0	52	from	dessert	117:123	arg1	differences					10:20	Exploring differences	0:20	Exploring differences in the physicochemical, functional, structural, and pasting properties of banana starches from dessert, cooking, and plantain cultivars (Musa spp.)	0:168	Exploring differences in the physicochemical, functional, structural, and pasting properties of banana starches from dessert, cooking, and plantain cultivars (Musa spp.)
34599989	10	53	theme	starch	1189:1194	arg1	digestibility					1196:1208	The in-vitro starch digestibility	1176:1208	The in-vitro starch digestibility	1176:1208	The in-vitro starch digestibility revealed that Saba starch has a lower hydrolysis rate with lesser glycemic index.
34599989	2	54	theme	cooking	453:459	arg1	cultivars					461:469	cooking cultivars	453:469	cooking cultivars	453:469	Herein, physico-chemical, and functional properties of banana starches isolated from dessert, plantain, and cooking cultivars were investigated.
34599989	6	55	dep	starch	732:737	arg1	95.86					740:744	95.86	740:744	95.86	740:744	Total starch (95.86 and 95.60%,) and resistant starch (65.56 and 59.20%) were higher in Saba and Monthan respectively.
34599989	6	55	dep	starch	732:737	arg1	%					755:755	95.60%	750:755	95.60%	750:755	Total starch (95.86 and 95.60%,) and resistant starch (65.56 and 59.20%) were higher in Saba and Monthan respectively.
34599989	1	56	theme	food	269:272	arg1	industry					274:281	the food industry	265:281	the food industry	265:281	Banana starch, with its nutritional and functional properties, opens up new opportunities for the food industry, which is seeking new starch sources to fulfil rising demand.
34599989	7	57	theme	banana	890:895	arg1	starches					897:904	banana starches	890:904	banana starches	890:904	Flour colour index (86.2-90.6) was higher in banana starches.
34599989	5	58	theme	amylose	673:679	arg1	content					681:687	the amylose content	669:687	the amylose content	669:687	Among the cultivars, the amylose content was ranged between 25.05 and 31.86%.
34599989	8	59	contain	have	1053:1056	arg2	property					1082:1089	a higher gelatinization property	1058:1089	a higher gelatinization property	1058:1089	Differential scanning calorimetry and rapid viscosity studies confirmed that starches from Saba (87.67 and 85.71 °C) Monthan (85.36 and 81.65 °C) have a higher gelatinization property.
34599989	8	59	contain	have	1053:1056	arg1	starches					984:991	starches	984:991	starches from Saba (87.67 and 85.71 °C) Monthan (85.36 and 81.65 °C)	984:1051	Differential scanning calorimetry and rapid viscosity studies confirmed that starches from Saba (87.67 and 85.71 °C) Monthan (85.36 and 81.65 °C) have a higher gelatinization property.
34599989	10	60	theme	in-vitro	1180:1187	arg1	digestibility					1196:1208	The in-vitro starch digestibility	1176:1208	The in-vitro starch digestibility	1176:1208	The in-vitro starch digestibility revealed that Saba starch has a lower hydrolysis rate with lesser glycemic index.
34599989	0	61	dep	cultivars	148:156	arg1	spp					164:166	Musa spp	159:166	Musa spp.	159:167	Exploring differences in the physicochemical, functional, structural, and pasting properties of banana starches from dessert, cooking, and plantain cultivars (Musa spp.)
34599989	0	62	theme	functional	46:55	arg1	properties					82:91	the physicochemical, functional, structural, and pasting properties	25:91	the physicochemical, functional, structural, and pasting properties of banana starches	25:110	Exploring differences in the physicochemical, functional, structural, and pasting properties of banana starches from dessert, cooking, and plantain cultivars (Musa spp.)
34599989	8	63	theme	rapid	945:949	arg1	viscosity					951:959	rapid viscosity	945:959	rapid viscosity	945:959	Differential scanning calorimetry and rapid viscosity studies confirmed that starches from Saba (87.67 and 85.71 °C) Monthan (85.36 and 81.65 °C) have a higher gelatinization property.
34599989	2	64	theme	starches	407:414	arg1	physico-chemical					353:368	physico-chemical	353:368	physico-chemical	353:368	Herein, physico-chemical, and functional properties of banana starches isolated from dessert, plantain, and cooking cultivars were investigated.
34599989	11	65	from	impact	1315:1320	arg1	grouping					1369:1376	the grouping	1365:1376	the grouping of varieties	1365:1389	PCA showed the greater impact of amylose and resistant starch content on the grouping of varieties.
34599989	12	66	theme	food	1427:1430	arg1	industries					1445:1454	food and non-food industries	1427:1454	food and non-food industries in terms of using banana starch in various food compositions and other industrial applications	1427:1549	These findings would be useful for food and non-food industries in terms of using banana starch in various food compositions and other industrial applications.
34599989	11	67	theme	resistant	1337:1345	arg1	content					1354:1360	resistant starch content	1337:1360	resistant starch content	1337:1360	PCA showed the greater impact of amylose and resistant starch content on the grouping of varieties.
34599989	0	68	from	cooking	126:132	arg1	differences					10:20	Exploring differences	0:20	Exploring differences in the physicochemical, functional, structural, and pasting properties of banana starches from dessert, cooking, and plantain cultivars (Musa spp.)	0:168	Exploring differences in the physicochemical, functional, structural, and pasting properties of banana starches from dessert, cooking, and plantain cultivars (Musa spp.)
34599989	0	69	theme	plantain	139:146	arg1	cultivars					148:156	plantain cultivars	139:156	plantain cultivars (Musa spp.)	139:168	Exploring differences in the physicochemical, functional, structural, and pasting properties of banana starches from dessert, cooking, and plantain cultivars (Musa spp.)
34599989	7	70	theme	colour	851:856	arg1	86.2-90.6					865:873	86.2-90.6	865:873	86.2-90.6	865:873	Flour colour index (86.2-90.6) was higher in banana starches.
34599989	7	70	theme	colour	851:856	arg1	index					858:862	Flour colour index	845:862	Flour colour index (86.2-90.6)	845:874	Flour colour index (86.2-90.6) was higher in banana starches.
34599989	8	71	theme	gelatinization	1067:1080	arg1	property					1082:1089	a higher gelatinization property	1058:1089	a higher gelatinization property	1058:1089	Differential scanning calorimetry and rapid viscosity studies confirmed that starches from Saba (87.67 and 85.71 °C) Monthan (85.36 and 81.65 °C) have a higher gelatinization property.
34599989	10	72	contain	has	1236:1238	arg1	starch					1229:1234	Saba starch	1224:1234	Saba starch	1224:1234	The in-vitro starch digestibility revealed that Saba starch has a lower hydrolysis rate with lesser glycemic index.
34599989	10	72	contain	has	1236:1238	arg2	rate					1259:1262	a lower hydrolysis rate	1240:1262	a lower hydrolysis rate with lesser glycemic index	1240:1289	The in-vitro starch digestibility revealed that Saba starch has a lower hydrolysis rate with lesser glycemic index.
34599989	4	73	theme	Starch	556:561	arg1	granules					563:570	Starch granules	556:570	Starch granules	556:570	Starch granules registered irregular forms with granule sizes ranging from 8.9 to 55.09 μm.
34599989	11	74	theme	amylose	1325:1331	arg1	impact					1315:1320	the greater impact	1303:1320	the greater impact of amylose and resistant starch content on the grouping of varieties	1303:1389	PCA showed the greater impact of amylose and resistant starch content on the grouping of varieties.
34599989	1	75	theme	nutritional	195:205	arg1	properties					222:231	its nutritional and functional properties	191:231	its nutritional and functional properties	191:231	Banana starch, with its nutritional and functional properties, opens up new opportunities for the food industry, which is seeking new starch sources to fulfil rising demand.
34599989	0	76	theme	structural	58:67	arg1	properties					82:91	the physicochemical, functional, structural, and pasting properties	25:91	the physicochemical, functional, structural, and pasting properties of banana starches	25:110	Exploring differences in the physicochemical, functional, structural, and pasting properties of banana starches from dessert, cooking, and plantain cultivars (Musa spp.)
34599989	9	77	theme	crystallinity	1139:1151	arg1	levels					1153:1158	crystallinity levels	1139:1158	crystallinity levels	1139:1158	Banana starches were B and C-type with varying crystallinity levels (21.19-52.01%).
34599989	4	78	theme	granule	604:610	arg1	sizes					612:616	granule sizes	604:616	granule sizes ranging from 8.9 to 55.09 μm	604:645	Starch granules registered irregular forms with granule sizes ranging from 8.9 to 55.09 μm.
34599989	12	79	theme	other	1521:1525	arg1	applications					1538:1549	other industrial applications	1521:1549	other industrial applications	1521:1549	These findings would be useful for food and non-food industries in terms of using banana starch in various food compositions and other industrial applications.
34599989	6	80	dep	starch	773:778	arg1	65.56					781:785	65.56	781:785	65.56	781:785	Total starch (95.86 and 95.60%,) and resistant starch (65.56 and 59.20%) were higher in Saba and Monthan respectively.
34599989	6	80	dep	starch	773:778	arg1	%					796:796	59.20%	791:796	59.20%	791:796	Total starch (95.86 and 95.60%,) and resistant starch (65.56 and 59.20%) were higher in Saba and Monthan respectively.
34287880	13	0	theme	bioactive	2122:2130	arg1	substances					2132:2141	bioactive substances	2122:2141	bioactive substances	2122:2141	Zein/starch sodium octenyl succinate composite nanoparticles formulated in this study provide novel stabilizers for emulsification systems or carriers of bioactive substances that can enhance the nutritional value, taste, or shelf life of foods.
34287880	3	1	theme	apparent	549:556	arg1	structures					558:567	apparent structures	549:567	apparent structures	549:567	We measured the particle size distribution, hydrophobicity, and apparent structures of the composite nanoparticles.
34287880	7	2	theme	three-phase	914:924	arg1	angle					934:938	three-phase contact angle	914:938	three-phase contact angle	914:938	The fluorescence spectrum and three-phase contact angle indicated that ZSPs presented hydrophilicity with largest three-phase contact angle, which was 65.1°.
34287880	5	3	theme	ZSP	715:717	arg1	nanoparticles					719:731	The ZSP nanoparticles	711:731	The ZSP nanoparticles	711:731	The ZSP nanoparticles were with an average particle size in the range of 70 to 110 nm.
34287880	10	4	theme	ultrasonic	1499:1508	arg1	treatment					1510:1518	ethanol combined ultrasonic treatment	1482:1518	ethanol combined ultrasonic treatment (10 min)	1482:1527	Differential scanning calorimetry analysis indicated that ethanol combined ultrasonic treatment (10 min) was beneficial to enhance the thermal stability of composite nanoparticles, causing the highest Tg of 153.6°C.
34287880	10	4	theme	ultrasonic	1499:1508	arg1	10 min					1521:1526	10 min	1521:1526	10 min	1521:1526	Differential scanning calorimetry analysis indicated that ethanol combined ultrasonic treatment (10 min) was beneficial to enhance the thermal stability of composite nanoparticles, causing the highest Tg of 153.6°C.
34287880	1	5	theme	precipitation	284:296	arg1	technology					298:307	anti-solvent precipitation technology	271:307	anti-solvent precipitation technology	271:307	The objective of this study is to prepare zein/starch sodium octenyl succinate composite nanoparticles (ZSPs) via anti-solvent precipitation technology and characterize their colloidal properties.
34287880	11	6	theme	practical	1668:1676	arg1	reference					1678:1686	a practical reference	1666:1686	a practical reference for formulating delivery systems using bioactive compounds containing zein as a carrier biopolymer	1666:1785	This work aims to provide a practical reference for formulating delivery systems using bioactive compounds containing zein as a carrier biopolymer.
34287880	3	7	theme	composite	576:584	arg1	nanoparticles					586:598	the composite nanoparticles	572:598	the composite nanoparticles	572:598	We measured the particle size distribution, hydrophobicity, and apparent structures of the composite nanoparticles.
34287880	8	8	theme	infrared	1060:1067	arg1	spectroscopy					1069:1080	infrared spectroscopy	1060:1080	infrared spectroscopy	1060:1080	Fourier transform infrared spectroscopy and scanning electron microscopy revealed that hydrophilic SSOS absorbed on the surface of zein nanoparticles via Van der Waals to improve their water solubility.
34287880	12	9	contain	containing	1927:1936	arg2	biopolymer					1956:1965	a carrier biopolymer	1946:1965	a carrier biopolymer	1946:1965	PRACTICAL APPLICATION: This work aims to provide a practical reference for formulating encapsulants for food and other bioactive compounds containing zein as a carrier biopolymer.
34287880	12	9	contain	containing	1927:1936	arg1	food					1892:1895	food	1892:1895	food	1892:1895	PRACTICAL APPLICATION: This work aims to provide a practical reference for formulating encapsulants for food and other bioactive compounds containing zein as a carrier biopolymer.
34287880	12	9	contain	containing	1927:1936	arg2	zein					1938:1941	zein	1938:1941	zein	1938:1941	PRACTICAL APPLICATION: This work aims to provide a practical reference for formulating encapsulants for food and other bioactive compounds containing zein as a carrier biopolymer.
34287880	6	10	theme	ultrasonic	807:816	arg1	time					828:831	the ultrasonic treatment time	803:831	the ultrasonic treatment time	803:831	When the ultrasonic treatment time exceeds 25 min, ZSPs became macroscopic particles.
34287880	5	11	theme	particle	754:761	arg1	size					763:766	an average particle size	743:766	an average particle size in the range of 70 to 110 nm	743:795	The ZSP nanoparticles were with an average particle size in the range of 70 to 110 nm.
34287880	1	12	theme	sodium	211:216	arg1	succinate					226:234	zein/starch sodium octenyl succinate	199:234	zein/starch sodium octenyl succinate composite nanoparticles (ZSPs)	199:265	The objective of this study is to prepare zein/starch sodium octenyl succinate composite nanoparticles (ZSPs) via anti-solvent precipitation technology and characterize their colloidal properties.
34287880	4	13	theme	nanoparticle	687:698	arg1	formation					700:708	composite nanoparticle formation	677:708	composite nanoparticle formation	677:708	Ultrasonic treatment time (0-25 min) was found to play an important role in composite nanoparticle formation.
34287880	0	14	theme	nanoparticles	142:154	arg1	properties					81:90	thermal and structural properties	58:90	thermal and structural properties of zein-starch sodium octenyl succinate composite nanoparticles	58:154	Effects of different alcohol and ultrasonic treatments on thermal and structural properties of zein-starch sodium octenyl succinate composite nanoparticles.
34287880	1	15	theme	succinate	226:234	arg1	ZSPs					261:264	ZSPs	261:264	ZSPs	261:264	The objective of this study is to prepare zein/starch sodium octenyl succinate composite nanoparticles (ZSPs) via anti-solvent precipitation technology and characterize their colloidal properties.
34287880	1	15	theme	succinate	226:234	arg1	nanoparticles					246:258	zein/starch sodium octenyl succinate composite nanoparticles	199:258	zein/starch sodium octenyl succinate composite nanoparticles (ZSPs)	199:265	The objective of this study is to prepare zein/starch sodium octenyl succinate composite nanoparticles (ZSPs) via anti-solvent precipitation technology and characterize their colloidal properties.
34287880	9	16	theme	main	1325:1328	arg1	changes					1249:1255	The changes	1245:1255	The changes in solvent polarity and zein self-assembly	1245:1298	The changes in solvent polarity and zein self-assembly are considered to be the main driving force for composite nanoparticles conformational transitions from α-helix to β-sheet.
34287880	9	16	theme	main	1325:1328	arg1	force					1338:1342	the main driving force	1321:1342	the main driving force for composite nanoparticles conformational transitions from α-helix to β-sheet	1321:1421	The changes in solvent polarity and zein self-assembly are considered to be the main driving force for composite nanoparticles conformational transitions from α-helix to β-sheet.
34287880	13	17	theme	Zein/starch	1968:1978	arg1	nanoparticles					2015:2027	Zein/starch sodium octenyl succinate composite nanoparticles	1968:2027	Zein/starch sodium octenyl succinate composite nanoparticles formulated in this study	1968:2052	Zein/starch sodium octenyl succinate composite nanoparticles formulated in this study provide novel stabilizers for emulsification systems or carriers of bioactive substances that can enhance the nutritional value, taste, or shelf life of foods.
34287880	12	18	theme	practical	1839:1847	arg1	reference					1849:1857	a practical reference	1837:1857	a practical reference for formulating encapsulants for food and other bioactive compounds containing zein as a carrier biopolymer	1837:1965	PRACTICAL APPLICATION: This work aims to provide a practical reference for formulating encapsulants for food and other bioactive compounds containing zein as a carrier biopolymer.
34287880	2	19	theme	succinate	456:464	arg1	concentrations					416:429	concentrations	416:429	concentrations of starch sodium octenyl succinate	416:464	The effects of polar solvents, ultrasonic treatment time, and concentrations of starch sodium octenyl succinate were investigated.
34287880	2	19	theme	succinate	456:464	arg1	effects					358:364	The effects	354:364	The effects of polar solvents	354:382	The effects of polar solvents, ultrasonic treatment time, and concentrations of starch sodium octenyl succinate were investigated.
34287880	2	19	theme	succinate	456:464	arg1	time					406:409	ultrasonic treatment time	385:409	ultrasonic treatment time	385:409	The effects of polar solvents, ultrasonic treatment time, and concentrations of starch sodium octenyl succinate were investigated.
34287880	13	20	theme	emulsification	2084:2097	arg1	systems					2099:2105	emulsification systems	2084:2105	emulsification systems	2084:2105	Zein/starch sodium octenyl succinate composite nanoparticles formulated in this study provide novel stabilizers for emulsification systems or carriers of bioactive substances that can enhance the nutritional value, taste, or shelf life of foods.
34287880	5	21	theme	110 nm	790:795	arg1	range					775:779	the range	771:779	the range of 70 to 110 nm	771:795	The ZSP nanoparticles were with an average particle size in the range of 70 to 110 nm.
34287880	9	22	theme	composite	1348:1356	arg1	nanoparticles					1358:1370	composite nanoparticles	1348:1370	composite nanoparticles conformational transitions from α-helix to β-sheet	1348:1421	The changes in solvent polarity and zein self-assembly are considered to be the main driving force for composite nanoparticles conformational transitions from α-helix to β-sheet.
34287880	10	23	theme	thermal	1559:1565	arg1	stability					1567:1575	the thermal stability	1555:1575	the thermal stability of composite nanoparticles	1555:1602	Differential scanning calorimetry analysis indicated that ethanol combined ultrasonic treatment (10 min) was beneficial to enhance the thermal stability of composite nanoparticles, causing the highest Tg of 153.6°C.
34287880	9	24	from	changes	1249:1255	arg1	polarity					1268:1275	solvent polarity	1260:1275	solvent polarity	1260:1275	The changes in solvent polarity and zein self-assembly are considered to be the main driving force for composite nanoparticles conformational transitions from α-helix to β-sheet.
34287880	9	24	from	changes	1249:1255	arg1	self-assembly					1286:1298	zein self-assembly	1281:1298	zein self-assembly	1281:1298	The changes in solvent polarity and zein self-assembly are considered to be the main driving force for composite nanoparticles conformational transitions from α-helix to β-sheet.
34287880	0	25	theme	structural	70:79	arg1	properties					81:90	thermal and structural properties	58:90	thermal and structural properties of zein-starch sodium octenyl succinate composite nanoparticles	58:154	Effects of different alcohol and ultrasonic treatments on thermal and structural properties of zein-starch sodium octenyl succinate composite nanoparticles.
34287880	9	26	theme	conformational	1372:1385	arg1	transitions					1387:1397	composite nanoparticles conformational transitions	1348:1397	composite nanoparticles conformational transitions from α-helix to β-sheet	1348:1421	The changes in solvent polarity and zein self-assembly are considered to be the main driving force for composite nanoparticles conformational transitions from α-helix to β-sheet.
34287880	8	27	theme	electron	1095:1102	arg1	microscopy					1104:1113	scanning electron microscopy	1086:1113	scanning electron microscopy	1086:1113	Fourier transform infrared spectroscopy and scanning electron microscopy revealed that hydrophilic SSOS absorbed on the surface of zein nanoparticles via Van der Waals to improve their water solubility.
34287880	0	28	from	Effects	0:6	arg1	properties					81:90	thermal and structural properties	58:90	thermal and structural properties of zein-starch sodium octenyl succinate composite nanoparticles	58:154	Effects of different alcohol and ultrasonic treatments on thermal and structural properties of zein-starch sodium octenyl succinate composite nanoparticles.
34287880	9	29	theme	solvent	1260:1266	arg1	polarity					1268:1275	solvent polarity	1260:1275	solvent polarity	1260:1275	The changes in solvent polarity and zein self-assembly are considered to be the main driving force for composite nanoparticles conformational transitions from α-helix to β-sheet.
34287880	13	30	theme	shelf	2193:2197	arg1	life					2199:2202	shelf life	2193:2202	shelf life	2193:2202	Zein/starch sodium octenyl succinate composite nanoparticles formulated in this study provide novel stabilizers for emulsification systems or carriers of bioactive substances that can enhance the nutritional value, taste, or shelf life of foods.
34287880	10	31	theme	nanoparticles	1590:1602	arg1	stability					1567:1575	the thermal stability	1555:1575	the thermal stability of composite nanoparticles	1555:1602	Differential scanning calorimetry analysis indicated that ethanol combined ultrasonic treatment (10 min) was beneficial to enhance the thermal stability of composite nanoparticles, causing the highest Tg of 153.6°C.
34287880	13	32	theme	composite	2005:2013	arg1	nanoparticles					2015:2027	Zein/starch sodium octenyl succinate composite nanoparticles	1968:2027	Zein/starch sodium octenyl succinate composite nanoparticles formulated in this study	1968:2052	Zein/starch sodium octenyl succinate composite nanoparticles formulated in this study provide novel stabilizers for emulsification systems or carriers of bioactive substances that can enhance the nutritional value, taste, or shelf life of foods.
34287880	0	33	theme	sodium	107:112	arg1	nanoparticles					142:154	zein-starch sodium octenyl succinate composite nanoparticles	95:154	zein-starch sodium octenyl succinate composite nanoparticles	95:154	Effects of different alcohol and ultrasonic treatments on thermal and structural properties of zein-starch sodium octenyl succinate composite nanoparticles.
34287880	8	34	theme	hydrophilic	1129:1139	arg1	SSOS					1141:1144	hydrophilic SSOS	1129:1144	hydrophilic SSOS absorbed on the surface of zein nanoparticles via Van der Waals	1129:1208	Fourier transform infrared spectroscopy and scanning electron microscopy revealed that hydrophilic SSOS absorbed on the surface of zein nanoparticles via Van der Waals to improve their water solubility.
34287880	7	35	theme	three-phase	998:1008	arg1	angle					1018:1022	largest three-phase contact angle	990:1022	largest three-phase contact angle	990:1022	The fluorescence spectrum and three-phase contact angle indicated that ZSPs presented hydrophilicity with largest three-phase contact angle, which was 65.1°.
34287880	4	36	theme	Ultrasonic	601:610	arg1	0-25 min					628:635	0-25 min	628:635	0-25 min	628:635	Ultrasonic treatment time (0-25 min) was found to play an important role in composite nanoparticle formation.
34287880	4	36	theme	Ultrasonic	601:610	arg1	time					622:625	Ultrasonic treatment time	601:625	Ultrasonic treatment time (0-25 min)	601:636	Ultrasonic treatment time (0-25 min) was found to play an important role in composite nanoparticle formation.
34287880	10	37	theme	highest	1617:1623	arg1	Tg					1625:1626	the highest Tg	1613:1626	the highest Tg of 153.6°C	1613:1637	Differential scanning calorimetry analysis indicated that ethanol combined ultrasonic treatment (10 min) was beneficial to enhance the thermal stability of composite nanoparticles, causing the highest Tg of 153.6°C.
34287880	8	38	dep	Fourier	1042:1048	arg1	transform					1050:1058	transform	1050:1058	transform infrared spectroscopy and scanning electron microscopy	1050:1113	Fourier transform infrared spectroscopy and scanning electron microscopy revealed that hydrophilic SSOS absorbed on the surface of zein nanoparticles via Van der Waals to improve their water solubility.
34287880	3	39	theme	particle	501:508	arg1	size					510:513	the particle size distribution, hydrophobicity, and apparent structures	497:567	size	510:513	We measured the particle size distribution, hydrophobicity, and apparent structures of the composite nanoparticles.
34287880	0	40	theme	different	11:19	arg1	alcohol					21:27	different alcohol	11:27	different alcohol	11:27	Effects of different alcohol and ultrasonic treatments on thermal and structural properties of zein-starch sodium octenyl succinate composite nanoparticles.
34287880	13	41	theme	octenyl	1987:1993	arg1	nanoparticles					2015:2027	Zein/starch sodium octenyl succinate composite nanoparticles	1968:2027	Zein/starch sodium octenyl succinate composite nanoparticles formulated in this study	1968:2052	Zein/starch sodium octenyl succinate composite nanoparticles formulated in this study provide novel stabilizers for emulsification systems or carriers of bioactive substances that can enhance the nutritional value, taste, or shelf life of foods.
34287880	2	42	theme	sodium	441:446	arg1	succinate					456:464	starch sodium octenyl succinate	434:464	starch sodium octenyl succinate	434:464	The effects of polar solvents, ultrasonic treatment time, and concentrations of starch sodium octenyl succinate were investigated.
34287880	1	43	theme	colloidal	332:340	arg1	properties					342:351	their colloidal properties	326:351	their colloidal properties	326:351	The objective of this study is to prepare zein/starch sodium octenyl succinate composite nanoparticles (ZSPs) via anti-solvent precipitation technology and characterize their colloidal properties.
34287880	0	44	theme	thermal	58:64	arg1	properties					81:90	thermal and structural properties	58:90	thermal and structural properties of zein-starch sodium octenyl succinate composite nanoparticles	58:154	Effects of different alcohol and ultrasonic treatments on thermal and structural properties of zein-starch sodium octenyl succinate composite nanoparticles.
34287880	10	45	theme	scanning	1437:1444	arg1	calorimetry					1446:1456	Differential scanning calorimetry	1424:1456	Differential scanning calorimetry analysis	1424:1465	Differential scanning calorimetry analysis indicated that ethanol combined ultrasonic treatment (10 min) was beneficial to enhance the thermal stability of composite nanoparticles, causing the highest Tg of 153.6°C.
34287880	2	46	theme	solvents	375:382	arg1	concentrations					416:429	concentrations	416:429	concentrations of starch sodium octenyl succinate	416:464	The effects of polar solvents, ultrasonic treatment time, and concentrations of starch sodium octenyl succinate were investigated.
34287880	2	46	theme	solvents	375:382	arg1	effects					358:364	The effects	354:364	The effects of polar solvents	354:382	The effects of polar solvents, ultrasonic treatment time, and concentrations of starch sodium octenyl succinate were investigated.
34287880	2	46	theme	solvents	375:382	arg1	time					406:409	ultrasonic treatment time	385:409	ultrasonic treatment time	385:409	The effects of polar solvents, ultrasonic treatment time, and concentrations of starch sodium octenyl succinate were investigated.
34287880	0	47	theme	treatments	44:53	arg1	Effects					0:6	Effects	0:6	Effects of different alcohol and ultrasonic treatments on thermal and structural properties of zein-starch sodium octenyl succinate composite nanoparticles.	0:155	Effects of different alcohol and ultrasonic treatments on thermal and structural properties of zein-starch sodium octenyl succinate composite nanoparticles.
34287880	8	48	theme	nanoparticles	1178:1190	arg1	surface					1162:1168	the surface	1158:1168	the surface of zein nanoparticles	1158:1190	Fourier transform infrared spectroscopy and scanning electron microscopy revealed that hydrophilic SSOS absorbed on the surface of zein nanoparticles via Van der Waals to improve their water solubility.
34287880	1	49	theme	composite	236:244	arg1	ZSPs					261:264	ZSPs	261:264	ZSPs	261:264	The objective of this study is to prepare zein/starch sodium octenyl succinate composite nanoparticles (ZSPs) via anti-solvent precipitation technology and characterize their colloidal properties.
34287880	1	49	theme	composite	236:244	arg1	nanoparticles					246:258	zein/starch sodium octenyl succinate composite nanoparticles	199:258	zein/starch sodium octenyl succinate composite nanoparticles (ZSPs)	199:265	The objective of this study is to prepare zein/starch sodium octenyl succinate composite nanoparticles (ZSPs) via anti-solvent precipitation technology and characterize their colloidal properties.
34287880	8	50	theme	Van	1196:1198	arg1	Waals					1204:1208	Van der Waals	1196:1208	Van der Waals	1196:1208	Fourier transform infrared spectroscopy and scanning electron microscopy revealed that hydrophilic SSOS absorbed on the surface of zein nanoparticles via Van der Waals to improve their water solubility.
34287880	6	51	theme	macroscopic	861:871	arg1	particles					873:881	macroscopic particles	861:881	macroscopic particles	861:881	When the ultrasonic treatment time exceeds 25 min, ZSPs became macroscopic particles.
34287880	11	52	theme	delivery	1704:1711	arg1	systems					1713:1719	delivery systems	1704:1719	delivery systems using bioactive compounds containing zein as a carrier biopolymer	1704:1785	This work aims to provide a practical reference for formulating delivery systems using bioactive compounds containing zein as a carrier biopolymer.
34287880	3	53	dep	size	510:513	arg1	distribution					515:526	distribution	515:526	distribution	515:526	We measured the particle size distribution, hydrophobicity, and apparent structures of the composite nanoparticles.
34287880	11	54	theme	carrier	1768:1774	arg1	zein					1758:1761	zein	1758:1761	zein	1758:1761	This work aims to provide a practical reference for formulating delivery systems using bioactive compounds containing zein as a carrier biopolymer.
34287880	11	54	theme	carrier	1768:1774	arg1	biopolymer					1776:1785	a carrier biopolymer	1766:1785	a carrier biopolymer	1766:1785	This work aims to provide a practical reference for formulating delivery systems using bioactive compounds containing zein as a carrier biopolymer.
34287880	11	55	theme	bioactive	1727:1735	arg1	compounds					1737:1745	bioactive compounds	1727:1745	bioactive compounds containing zein as a carrier biopolymer	1727:1785	This work aims to provide a practical reference for formulating delivery systems using bioactive compounds containing zein as a carrier biopolymer.
34287880	2	56	theme	ultrasonic	385:394	arg1	time					406:409	ultrasonic treatment time	385:409	ultrasonic treatment time	385:409	The effects of polar solvents, ultrasonic treatment time, and concentrations of starch sodium octenyl succinate were investigated.
34287880	5	57	from	size	763:766	arg1	range					775:779	the range	771:779	the range of 70 to 110 nm	771:795	The ZSP nanoparticles were with an average particle size in the range of 70 to 110 nm.
34287880	9	58	theme	zein	1281:1284	arg1	self-assembly					1286:1298	zein self-assembly	1281:1298	zein self-assembly	1281:1298	The changes in solvent polarity and zein self-assembly are considered to be the main driving force for composite nanoparticles conformational transitions from α-helix to β-sheet.
34287880	13	59	theme	substances	2132:2141	arg1	carriers					2110:2117	carriers	2110:2117	carriers of bioactive substances	2110:2141	Zein/starch sodium octenyl succinate composite nanoparticles formulated in this study provide novel stabilizers for emulsification systems or carriers of bioactive substances that can enhance the nutritional value, taste, or shelf life of foods.
34287880	13	59	theme	substances	2132:2141	arg1	systems					2099:2105	emulsification systems	2084:2105	emulsification systems	2084:2105	Zein/starch sodium octenyl succinate composite nanoparticles formulated in this study provide novel stabilizers for emulsification systems or carriers of bioactive substances that can enhance the nutritional value, taste, or shelf life of foods.
34287880	3	60	theme	nanoparticles	586:598	arg1	hydrophobicity					529:542	hydrophobicity	529:542	hydrophobicity	529:542	We measured the particle size distribution, hydrophobicity, and apparent structures of the composite nanoparticles.
34287880	3	60	theme	nanoparticles	586:598	arg1	structures					558:567	apparent structures	549:567	apparent structures	549:567	We measured the particle size distribution, hydrophobicity, and apparent structures of the composite nanoparticles.
34287880	3	60	theme	nanoparticles	586:598	arg1	size					510:513	the particle size distribution, hydrophobicity, and apparent structures	497:567	size	510:513	We measured the particle size distribution, hydrophobicity, and apparent structures of the composite nanoparticles.
34287880	7	61	theme	contact	926:932	arg1	angle					934:938	three-phase contact angle	914:938	three-phase contact angle	914:938	The fluorescence spectrum and three-phase contact angle indicated that ZSPs presented hydrophilicity with largest three-phase contact angle, which was 65.1°.
34287880	1	62	theme	anti-solvent	271:282	arg1	technology					298:307	anti-solvent precipitation technology	271:307	anti-solvent precipitation technology	271:307	The objective of this study is to prepare zein/starch sodium octenyl succinate composite nanoparticles (ZSPs) via anti-solvent precipitation technology and characterize their colloidal properties.
34287880	8	63	theme	water	1227:1231	arg1	solubility					1233:1242	their water solubility	1221:1242	their water solubility	1221:1242	Fourier transform infrared spectroscopy and scanning electron microscopy revealed that hydrophilic SSOS absorbed on the surface of zein nanoparticles via Van der Waals to improve their water solubility.
34287880	12	64	theme	other	1901:1905	arg1	compounds					1917:1925	other bioactive compounds	1901:1925	other bioactive compounds	1901:1925	PRACTICAL APPLICATION: This work aims to provide a practical reference for formulating encapsulants for food and other bioactive compounds containing zein as a carrier biopolymer.
34287880	10	65	theme	combined	1490:1497	arg1	treatment					1510:1518	ethanol combined ultrasonic treatment	1482:1518	ethanol combined ultrasonic treatment (10 min)	1482:1527	Differential scanning calorimetry analysis indicated that ethanol combined ultrasonic treatment (10 min) was beneficial to enhance the thermal stability of composite nanoparticles, causing the highest Tg of 153.6°C.
34287880	10	65	theme	combined	1490:1497	arg1	10 min					1521:1526	10 min	1521:1526	10 min	1521:1526	Differential scanning calorimetry analysis indicated that ethanol combined ultrasonic treatment (10 min) was beneficial to enhance the thermal stability of composite nanoparticles, causing the highest Tg of 153.6°C.
34287880	1	66	theme	study	179:183	arg1	objective					161:169	The objective	157:169	The objective of this study	157:183	The objective of this study is to prepare zein/starch sodium octenyl succinate composite nanoparticles (ZSPs) via anti-solvent precipitation technology and characterize their colloidal properties.
34287880	6	67	theme	treatment	818:826	arg1	time					828:831	the ultrasonic treatment time	803:831	the ultrasonic treatment time	803:831	When the ultrasonic treatment time exceeds 25 min, ZSPs became macroscopic particles.
34287880	7	68	theme	fluorescence	888:899	arg1	spectrum					901:908	The fluorescence spectrum	884:908	The fluorescence spectrum	884:908	The fluorescence spectrum and three-phase contact angle indicated that ZSPs presented hydrophilicity with largest three-phase contact angle, which was 65.1°.
34287880	8	69	theme	scanning	1086:1093	arg1	microscopy					1104:1113	scanning electron microscopy	1086:1113	scanning electron microscopy	1086:1113	Fourier transform infrared spectroscopy and scanning electron microscopy revealed that hydrophilic SSOS absorbed on the surface of zein nanoparticles via Van der Waals to improve their water solubility.
34287880	0	70	theme	composite	132:140	arg1	nanoparticles					142:154	zein-starch sodium octenyl succinate composite nanoparticles	95:154	zein-starch sodium octenyl succinate composite nanoparticles	95:154	Effects of different alcohol and ultrasonic treatments on thermal and structural properties of zein-starch sodium octenyl succinate composite nanoparticles.
34287880	1	71	theme	zein/starch	199:209	arg1	succinate					226:234	zein/starch sodium octenyl succinate	199:234	zein/starch sodium octenyl succinate composite nanoparticles (ZSPs)	199:265	The objective of this study is to prepare zein/starch sodium octenyl succinate composite nanoparticles (ZSPs) via anti-solvent precipitation technology and characterize their colloidal properties.
34287880	9	72	from	α-helix	1404:1410	arg1	transitions					1387:1397	composite nanoparticles conformational transitions	1348:1397	composite nanoparticles conformational transitions from α-helix to β-sheet	1348:1421	The changes in solvent polarity and zein self-assembly are considered to be the main driving force for composite nanoparticles conformational transitions from α-helix to β-sheet.
34287880	11	73	contain	containing	1747:1756	arg2	zein					1758:1761	zein	1758:1761	zein	1758:1761	This work aims to provide a practical reference for formulating delivery systems using bioactive compounds containing zein as a carrier biopolymer.
34287880	11	73	contain	containing	1747:1756	arg1	compounds					1737:1745	bioactive compounds	1727:1745	bioactive compounds containing zein as a carrier biopolymer	1727:1785	This work aims to provide a practical reference for formulating delivery systems using bioactive compounds containing zein as a carrier biopolymer.
34287880	11	73	contain	containing	1747:1756	arg2	biopolymer					1776:1785	a carrier biopolymer	1766:1785	a carrier biopolymer	1766:1785	This work aims to provide a practical reference for formulating delivery systems using bioactive compounds containing zein as a carrier biopolymer.
34287880	1	74	theme	octenyl	218:224	arg1	succinate					226:234	zein/starch sodium octenyl succinate	199:234	zein/starch sodium octenyl succinate composite nanoparticles (ZSPs)	199:265	The objective of this study is to prepare zein/starch sodium octenyl succinate composite nanoparticles (ZSPs) via anti-solvent precipitation technology and characterize their colloidal properties.
34287880	5	75	dep	110 nm	790:795	arg1	to					787:788	to	787:788	to	787:788	The ZSP nanoparticles were with an average particle size in the range of 70 to 110 nm.
34287880	4	76	theme	composite	677:685	arg1	formation					700:708	composite nanoparticle formation	677:708	composite nanoparticle formation	677:708	Ultrasonic treatment time (0-25 min) was found to play an important role in composite nanoparticle formation.
34287880	5	77	theme	average	746:752	arg1	size					763:766	an average particle size	743:766	an average particle size in the range of 70 to 110 nm	743:795	The ZSP nanoparticles were with an average particle size in the range of 70 to 110 nm.
34287880	13	78	theme	novel	2062:2066	arg1	stabilizers					2068:2078	novel stabilizers	2062:2078	novel stabilizers for emulsification systems or carriers of bioactive substances that can enhance the nutritional value, taste, or shelf life of foods	2062:2211	Zein/starch sodium octenyl succinate composite nanoparticles formulated in this study provide novel stabilizers for emulsification systems or carriers of bioactive substances that can enhance the nutritional value, taste, or shelf life of foods.
34287880	9	79	theme	driving	1330:1336	arg1	changes					1249:1255	The changes	1245:1255	The changes in solvent polarity and zein self-assembly	1245:1298	The changes in solvent polarity and zein self-assembly are considered to be the main driving force for composite nanoparticles conformational transitions from α-helix to β-sheet.
34287880	9	79	theme	driving	1330:1336	arg1	force					1338:1342	the main driving force	1321:1342	the main driving force for composite nanoparticles conformational transitions from α-helix to β-sheet	1321:1421	The changes in solvent polarity and zein self-assembly are considered to be the main driving force for composite nanoparticles conformational transitions from α-helix to β-sheet.
34287880	9	80	theme	nanoparticles	1358:1370	arg1	transitions					1387:1397	composite nanoparticles conformational transitions	1348:1397	composite nanoparticles conformational transitions from α-helix to β-sheet	1348:1421	The changes in solvent polarity and zein self-assembly are considered to be the main driving force for composite nanoparticles conformational transitions from α-helix to β-sheet.
34287880	0	81	theme	succinate	122:130	arg1	nanoparticles					142:154	zein-starch sodium octenyl succinate composite nanoparticles	95:154	zein-starch sodium octenyl succinate composite nanoparticles	95:154	Effects of different alcohol and ultrasonic treatments on thermal and structural properties of zein-starch sodium octenyl succinate composite nanoparticles.
34287880	0	82	theme	zein-starch	95:105	arg1	nanoparticles					142:154	zein-starch sodium octenyl succinate composite nanoparticles	95:154	zein-starch sodium octenyl succinate composite nanoparticles	95:154	Effects of different alcohol and ultrasonic treatments on thermal and structural properties of zein-starch sodium octenyl succinate composite nanoparticles.
34287880	12	83	dep	APPLICATION	1798:1808	arg1	aims					1821:1824	aims	1821:1824	aims to provide a practical reference for formulating encapsulants for food and other bioactive compounds containing zein as a carrier biopolymer	1821:1965	PRACTICAL APPLICATION: This work aims to provide a practical reference for formulating encapsulants for food and other bioactive compounds containing zein as a carrier biopolymer.
34287880	13	84	theme	succinate	1995:2003	arg1	nanoparticles					2015:2027	Zein/starch sodium octenyl succinate composite nanoparticles	1968:2027	Zein/starch sodium octenyl succinate composite nanoparticles formulated in this study	1968:2052	Zein/starch sodium octenyl succinate composite nanoparticles formulated in this study provide novel stabilizers for emulsification systems or carriers of bioactive substances that can enhance the nutritional value, taste, or shelf life of foods.
34287880	0	85	theme	octenyl	114:120	arg1	nanoparticles					142:154	zein-starch sodium octenyl succinate composite nanoparticles	95:154	zein-starch sodium octenyl succinate composite nanoparticles	95:154	Effects of different alcohol and ultrasonic treatments on thermal and structural properties of zein-starch sodium octenyl succinate composite nanoparticles.
34287880	10	86	theme	composite	1580:1588	arg1	nanoparticles					1590:1602	composite nanoparticles	1580:1602	composite nanoparticles	1580:1602	Differential scanning calorimetry analysis indicated that ethanol combined ultrasonic treatment (10 min) was beneficial to enhance the thermal stability of composite nanoparticles, causing the highest Tg of 153.6°C.
34287880	13	87	theme	foods	2207:2211	arg1	taste					2183:2187	taste	2183:2187	taste	2183:2187	Zein/starch sodium octenyl succinate composite nanoparticles formulated in this study provide novel stabilizers for emulsification systems or carriers of bioactive substances that can enhance the nutritional value, taste, or shelf life of foods.
34287880	13	87	theme	foods	2207:2211	arg1	value					2176:2180	the nutritional value	2160:2180	the nutritional value	2160:2180	Zein/starch sodium octenyl succinate composite nanoparticles formulated in this study provide novel stabilizers for emulsification systems or carriers of bioactive substances that can enhance the nutritional value, taste, or shelf life of foods.
34287880	13	87	theme	foods	2207:2211	arg1	life					2199:2202	shelf life	2193:2202	shelf life	2193:2202	Zein/starch sodium octenyl succinate composite nanoparticles formulated in this study provide novel stabilizers for emulsification systems or carriers of bioactive substances that can enhance the nutritional value, taste, or shelf life of foods.
34287880	7	88	theme	contact	1010:1016	arg1	angle					1018:1022	largest three-phase contact angle	990:1022	largest three-phase contact angle	990:1022	The fluorescence spectrum and three-phase contact angle indicated that ZSPs presented hydrophilicity with largest three-phase contact angle, which was 65.1°.
34287880	12	89	theme	PRACTICAL	1788:1796	arg1	APPLICATION					1798:1808	PRACTICAL APPLICATION	1788:1808	PRACTICAL APPLICATION: This work aims to provide a practical reference for formulating encapsulants for food and other bioactive compounds containing zein as a carrier biopolymer.	1788:1966	PRACTICAL APPLICATION: This work aims to provide a practical reference for formulating encapsulants for food and other bioactive compounds containing zein as a carrier biopolymer.
34287880	4	90	theme	treatment	612:620	arg1	0-25 min					628:635	0-25 min	628:635	0-25 min	628:635	Ultrasonic treatment time (0-25 min) was found to play an important role in composite nanoparticle formation.
34287880	4	90	theme	treatment	612:620	arg1	time					622:625	Ultrasonic treatment time	601:625	Ultrasonic treatment time (0-25 min)	601:636	Ultrasonic treatment time (0-25 min) was found to play an important role in composite nanoparticle formation.
34287880	0	91	theme	alcohol	21:27	arg1	Effects					0:6	Effects	0:6	Effects of different alcohol and ultrasonic treatments on thermal and structural properties of zein-starch sodium octenyl succinate composite nanoparticles.	0:155	Effects of different alcohol and ultrasonic treatments on thermal and structural properties of zein-starch sodium octenyl succinate composite nanoparticles.
34287880	7	92	theme	largest	990:996	arg1	angle					1018:1022	largest three-phase contact angle	990:1022	largest three-phase contact angle	990:1022	The fluorescence spectrum and three-phase contact angle indicated that ZSPs presented hydrophilicity with largest three-phase contact angle, which was 65.1°.
34287880	13	93	theme	sodium	1980:1985	arg1	nanoparticles					2015:2027	Zein/starch sodium octenyl succinate composite nanoparticles	1968:2027	Zein/starch sodium octenyl succinate composite nanoparticles formulated in this study	1968:2052	Zein/starch sodium octenyl succinate composite nanoparticles formulated in this study provide novel stabilizers for emulsification systems or carriers of bioactive substances that can enhance the nutritional value, taste, or shelf life of foods.
34287880	2	94	theme	octenyl	448:454	arg1	succinate					456:464	starch sodium octenyl succinate	434:464	starch sodium octenyl succinate	434:464	The effects of polar solvents, ultrasonic treatment time, and concentrations of starch sodium octenyl succinate were investigated.
34287880	0	95	theme	ultrasonic	33:42	arg1	treatments					44:53	ultrasonic treatments	33:53	ultrasonic treatments	33:53	Effects of different alcohol and ultrasonic treatments on thermal and structural properties of zein-starch sodium octenyl succinate composite nanoparticles.
34287880	8	96	theme	zein	1173:1176	arg1	nanoparticles					1178:1190	zein nanoparticles	1173:1190	zein nanoparticles	1173:1190	Fourier transform infrared spectroscopy and scanning electron microscopy revealed that hydrophilic SSOS absorbed on the surface of zein nanoparticles via Van der Waals to improve their water solubility.
34287880	10	97	theme	calorimetry	1446:1456	arg1	analysis					1458:1465	Differential scanning calorimetry analysis	1424:1465	Differential scanning calorimetry analysis	1424:1465	Differential scanning calorimetry analysis indicated that ethanol combined ultrasonic treatment (10 min) was beneficial to enhance the thermal stability of composite nanoparticles, causing the highest Tg of 153.6°C.
34287880	2	98	theme	starch	434:439	arg1	succinate					456:464	starch sodium octenyl succinate	434:464	starch sodium octenyl succinate	434:464	The effects of polar solvents, ultrasonic treatment time, and concentrations of starch sodium octenyl succinate were investigated.
34287880	10	99	theme	153.6°C	1631:1637	arg1	Tg					1625:1626	the highest Tg	1613:1626	the highest Tg of 153.6°C	1613:1637	Differential scanning calorimetry analysis indicated that ethanol combined ultrasonic treatment (10 min) was beneficial to enhance the thermal stability of composite nanoparticles, causing the highest Tg of 153.6°C.
34287880	10	100	theme	Differential	1424:1435	arg1	calorimetry					1446:1456	Differential scanning calorimetry	1424:1456	Differential scanning calorimetry analysis	1424:1465	Differential scanning calorimetry analysis indicated that ethanol combined ultrasonic treatment (10 min) was beneficial to enhance the thermal stability of composite nanoparticles, causing the highest Tg of 153.6°C.
34287880	2	101	theme	polar	369:373	arg1	solvents					375:382	polar solvents	369:382	polar solvents	369:382	The effects of polar solvents, ultrasonic treatment time, and concentrations of starch sodium octenyl succinate were investigated.
34287880	8	102	theme	der	1200:1202	arg1	Waals					1204:1208	Van der Waals	1196:1208	Van der Waals	1196:1208	Fourier transform infrared spectroscopy and scanning electron microscopy revealed that hydrophilic SSOS absorbed on the surface of zein nanoparticles via Van der Waals to improve their water solubility.
34287880	12	103	theme	carrier	1948:1954	arg1	zein					1938:1941	zein	1938:1941	zein	1938:1941	PRACTICAL APPLICATION: This work aims to provide a practical reference for formulating encapsulants for food and other bioactive compounds containing zein as a carrier biopolymer.
34287880	12	103	theme	carrier	1948:1954	arg1	biopolymer					1956:1965	a carrier biopolymer	1946:1965	a carrier biopolymer	1946:1965	PRACTICAL APPLICATION: This work aims to provide a practical reference for formulating encapsulants for food and other bioactive compounds containing zein as a carrier biopolymer.
34287880	10	104	theme	ethanol	1482:1488	arg1	treatment					1510:1518	ethanol combined ultrasonic treatment	1482:1518	ethanol combined ultrasonic treatment (10 min)	1482:1527	Differential scanning calorimetry analysis indicated that ethanol combined ultrasonic treatment (10 min) was beneficial to enhance the thermal stability of composite nanoparticles, causing the highest Tg of 153.6°C.
34287880	10	104	theme	ethanol	1482:1488	arg1	10 min					1521:1526	10 min	1521:1526	10 min	1521:1526	Differential scanning calorimetry analysis indicated that ethanol combined ultrasonic treatment (10 min) was beneficial to enhance the thermal stability of composite nanoparticles, causing the highest Tg of 153.6°C.
34287880	12	105	theme	bioactive	1907:1915	arg1	compounds					1917:1925	other bioactive compounds	1901:1925	other bioactive compounds	1901:1925	PRACTICAL APPLICATION: This work aims to provide a practical reference for formulating encapsulants for food and other bioactive compounds containing zein as a carrier biopolymer.
34287880	4	106	theme	important	659:667	arg1	role					669:672	an important role	656:672	an important role	656:672	Ultrasonic treatment time (0-25 min) was found to play an important role in composite nanoparticle formation.
34287880	13	107	theme	nutritional	2164:2174	arg1	value					2176:2180	the nutritional value	2160:2180	the nutritional value	2160:2180	Zein/starch sodium octenyl succinate composite nanoparticles formulated in this study provide novel stabilizers for emulsification systems or carriers of bioactive substances that can enhance the nutritional value, taste, or shelf life of foods.
34287880	2	108	theme	treatment	396:404	arg1	time					406:409	ultrasonic treatment time	385:409	ultrasonic treatment time	385:409	The effects of polar solvents, ultrasonic treatment time, and concentrations of starch sodium octenyl succinate were investigated.
32219624	0	0	theme	Beta	103:106	arg1	Strands					108:114	Central Beta Strands	95:114	Central Beta Strands of TIM Barrel	95:128	Simultaneously Improved Thermostability and Hydrolytic Pattern of Alpha-Amylase by Engineering Central Beta Strands of TIM Barrel.
32219624	4	1	from	endo-amylase	879:890	arg1	conversion					855:864	a conversion	853:864	a conversion from typical endo-amylase to novel maltooligosaccharide-producing amylase	853:938	A mutant Y204V produced only maltose, maltotriose, and maltopentaose without any glucose and maltotetraose, indicating a conversion from typical endo-amylase to novel maltooligosaccharide-producing amylase.
32219624	4	2	theme	typical	871:877	arg1	endo-amylase					879:890	typical endo-amylase	871:890	typical endo-amylase	871:890	A mutant Y204V produced only maltose, maltotriose, and maltopentaose without any glucose and maltotetraose, indicating a conversion from typical endo-amylase to novel maltooligosaccharide-producing amylase.
32219624	5	3	theme	protein	1257:1263	arg1	engineering					1265:1275	the protein engineering	1253:1275	the protein engineering of other TIM barrel proteins	1253:1304	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	5	4	theme	"	1210:1210	arg1	useful					1242:1247	useful	1242:1247	useful	1242:1247	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	5	4	theme	"	1210:1210	arg1	strategy					1212:1219	the novel "beta strands" strategy	1187:1219	the novel "beta strands" strategy proposed here	1187:1233	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	0	5	theme	Central	95:101	arg1	Strands					108:114	Central Beta Strands	95:114	Central Beta Strands of TIM Barrel	95:128	Simultaneously Improved Thermostability and Hydrolytic Pattern of Alpha-Amylase by Engineering Central Beta Strands of TIM Barrel.
32219624	5	6	theme	thermostability	1081:1095	arg1	improvement					1066:1076	the simultaneous improvement	1049:1076	the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase	1049:1131	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	2	7	theme	double	376:381	arg1	mutation					383:390	a double mutation	374:390	a double mutation	374:390	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	1	8	theme	conserved	291:299	arg1	strands					314:320	the mostly conserved central beta strands	280:320	the mostly conserved central beta strands in TIM barrel fold	280:339	This study reported simultaneously improved thermostability and hydrolytic pattern of α-amylase from Bacillus subtilis CN7 by rationally engineering the mostly conserved central beta strands in TIM barrel fold.
32219624	3	9	from	percentages	617:627	arg1	products					674:681	final hydrolytic products	657:681	final hydrolytic products	657:681	All the five active mutants changed the compositions and percentages of maltooligosaccharides in final hydrolytic products compared to the product spectrum of the wild-type.
32219624	5	10	theme	central	1148:1154	arg1	strands					1161:1167	central beta strands	1148:1167	central beta strands of TIM barrel	1148:1181	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	5	11	theme	pattern	1112:1118	arg1	improvement					1066:1076	the simultaneous improvement	1049:1076	the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase	1049:1131	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	1	12	theme	central	301:307	arg1	strands					314:320	the mostly conserved central beta strands	280:320	the mostly conserved central beta strands in TIM barrel fold	280:339	This study reported simultaneously improved thermostability and hydrolytic pattern of α-amylase from Bacillus subtilis CN7 by rationally engineering the mostly conserved central beta strands in TIM barrel fold.
32219624	2	13	theme	beta	515:518	arg1	strands					520:526	the beta strands	511:526	the beta strands of the TIM barrel of α-amylase	511:557	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	3	14	theme	wild-type	723:731	arg1	spectrum					707:714	the product spectrum	695:714	the product spectrum of the wild-type	695:731	All the five active mutants changed the compositions and percentages of maltooligosaccharides in final hydrolytic products compared to the product spectrum of the wild-type.
32219624	1	15	dep	reported	142:149	arg1	improved					166:173	improved	166:173	reported simultaneously improved thermostability and hydrolytic pattern of α-amylase from Bacillus subtilis CN7 by rationally engineering the mostly conserved central beta strands in TIM barrel fold	142:339	This study reported simultaneously improved thermostability and hydrolytic pattern of α-amylase from Bacillus subtilis CN7 by rationally engineering the mostly conserved central beta strands in TIM barrel fold.
32219624	1	16	theme	beta	309:312	arg1	strands					314:320	the mostly conserved central beta strands	280:320	the mostly conserved central beta strands in TIM barrel fold	280:339	This study reported simultaneously improved thermostability and hydrolytic pattern of α-amylase from Bacillus subtilis CN7 by rationally engineering the mostly conserved central beta strands in TIM barrel fold.
32219624	0	17	theme	Barrel	123:128	arg1	Strands					108:114	Central Beta Strands	95:114	Central Beta Strands of TIM Barrel	95:128	Simultaneously Improved Thermostability and Hydrolytic Pattern of Alpha-Amylase by Engineering Central Beta Strands of TIM Barrel.
32219624	5	18	theme	α-amylase	1123:1131	arg1	thermostability					1081:1095	thermostability	1081:1095	thermostability	1081:1095	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	5	18	theme	α-amylase	1123:1131	arg1	pattern					1112:1118	hydrolytic pattern	1101:1118	hydrolytic pattern	1101:1118	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	2	19	theme	β7	431:432	arg1	strand					434:439	the β7 strand	427:439	the β7 strand	427:439	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	1	20	theme	hydrolytic	195:204	arg1	pattern					206:212	hydrolytic pattern	195:212	hydrolytic pattern	195:212	This study reported simultaneously improved thermostability and hydrolytic pattern of α-amylase from Bacillus subtilis CN7 by rationally engineering the mostly conserved central beta strands in TIM barrel fold.
32219624	0	21	theme	TIM	119:121	arg1	Barrel					123:128	TIM Barrel	119:128	TIM Barrel	119:128	Simultaneously Improved Thermostability and Hydrolytic Pattern of Alpha-Amylase by Engineering Central Beta Strands of TIM Barrel.
32219624	5	22	theme	beta	1156:1159	arg1	strands					1161:1167	central beta strands	1148:1167	central beta strands of TIM barrel	1148:1181	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	5	23	theme	beta	1198:1201	arg1	useful					1242:1247	useful	1242:1247	useful	1242:1247	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	5	23	theme	beta	1198:1201	arg1	strategy					1212:1219	the novel "beta strands" strategy	1187:1219	the novel "beta strands" strategy proposed here	1187:1233	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	5	24	theme	best	1005:1008	arg1	knowledge					1010:1018	our best knowledge	1001:1018	our best knowledge	1001:1018	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	3	25	from	compositions	600:611	arg1	products					674:681	final hydrolytic products	657:681	final hydrolytic products	657:681	All the five active mutants changed the compositions and percentages of maltooligosaccharides in final hydrolytic products compared to the product spectrum of the wild-type.
32219624	2	26	theme	2nd	415:417	arg1	site					419:422	the 2nd site	411:422	the 2nd site of the β7 strand	411:439	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	2	26	theme	2nd	415:417	arg1	strand					464:469	the β5 strand	457:469	the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase	457:557	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	2	26	theme	2nd	415:417	arg1	strand					434:439	the β7 strand	427:439	the β7 strand	427:439	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	5	27	theme	strands	1203:1209	arg1	useful					1242:1247	useful	1242:1247	useful	1242:1247	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	5	27	theme	strands	1203:1209	arg1	strategy					1212:1219	the novel "beta strands" strategy	1187:1219	the novel "beta strands" strategy proposed here	1187:1233	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	1	28	from	strands	314:320	arg1	fold					336:339	TIM barrel fold	325:339	TIM barrel fold	325:339	This study reported simultaneously improved thermostability and hydrolytic pattern of α-amylase from Bacillus subtilis CN7 by rationally engineering the mostly conserved central beta strands in TIM barrel fold.
32219624	2	29	theme	single	347:352	arg1	mutations					360:368	Nine single point mutations	342:368	Nine single point mutations	342:368	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	5	30	theme	novel	1191:1195	arg1	useful					1242:1247	useful	1242:1247	useful	1242:1247	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	5	30	theme	novel	1191:1195	arg1	strategy					1212:1219	the novel "beta strands" strategy	1187:1219	the novel "beta strands" strategy proposed here	1187:1233	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	5	31	theme	thermal	969:975	arg1	stability					977:985	the thermal stability	965:985	the thermal stability by 7.1 °C. To our best knowledge	965:1018	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	5	32	from	report	1039:1044	arg1	improvement					1066:1076	the simultaneous improvement	1049:1076	the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase	1049:1131	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	1	33	theme	α-amylase	217:225	arg1	thermostability					175:189	thermostability	175:189	thermostability	175:189	This study reported simultaneously improved thermostability and hydrolytic pattern of α-amylase from Bacillus subtilis CN7 by rationally engineering the mostly conserved central beta strands in TIM barrel fold.
32219624	1	33	theme	α-amylase	217:225	arg1	pattern					206:212	hydrolytic pattern	195:212	hydrolytic pattern	195:212	This study reported simultaneously improved thermostability and hydrolytic pattern of α-amylase from Bacillus subtilis CN7 by rationally engineering the mostly conserved central beta strands in TIM barrel fold.
32219624	2	34	theme	3rd	445:447	arg1	site					449:452	3rd site	445:452	3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase	445:557	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	2	34	theme	3rd	445:447	arg1	strand					464:469	the β5 strand	457:469	the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase	457:557	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	2	34	theme	3rd	445:447	arg1	strand					434:439	the β7 strand	427:439	the β7 strand	427:439	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	2	35	theme	barrel	539:544	arg1	strands					520:526	the beta strands	511:526	the beta strands of the TIM barrel of α-amylase	511:557	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	2	36	theme	strand	434:439	arg1	site					449:452	3rd site	445:452	3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase	445:557	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	2	36	theme	strand	434:439	arg1	site					419:422	the 2nd site	411:422	the 2nd site of the β7 strand	411:439	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	2	36	theme	strand	434:439	arg1	strand					464:469	the β5 strand	457:469	the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase	457:557	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	2	36	theme	strand	434:439	arg1	strand					434:439	the β7 strand	427:439	the β7 strand	427:439	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	5	37	theme	mutant	943:948	arg1	V260I					950:954	A mutant V260I	941:954	A mutant V260I	941:954	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	1	38	theme	TIM	325:327	arg1	fold					336:339	TIM barrel fold	325:339	TIM barrel fold	325:339	This study reported simultaneously improved thermostability and hydrolytic pattern of α-amylase from Bacillus subtilis CN7 by rationally engineering the mostly conserved central beta strands in TIM barrel fold.
32219624	2	39	theme	TIM	535:537	arg1	barrel					539:544	the TIM barrel	531:544	the TIM barrel of α-amylase	531:557	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	3	40	theme	maltooligosaccharides	632:652	arg1	percentages					617:627	percentages	617:627	percentages	617:627	All the five active mutants changed the compositions and percentages of maltooligosaccharides in final hydrolytic products compared to the product spectrum of the wild-type.
32219624	3	40	theme	maltooligosaccharides	632:652	arg1	compositions					600:611	compositions	600:611	compositions	600:611	All the five active mutants changed the compositions and percentages of maltooligosaccharides in final hydrolytic products compared to the product spectrum of the wild-type.
32219624	1	41	theme	Bacillus	232:239	arg1	CN7					250:252	Bacillus subtilis CN7	232:252	Bacillus subtilis CN7	232:252	This study reported simultaneously improved thermostability and hydrolytic pattern of α-amylase from Bacillus subtilis CN7 by rationally engineering the mostly conserved central beta strands in TIM barrel fold.
32219624	1	42	theme	barrel	329:334	arg1	fold					336:339	TIM barrel fold	325:339	TIM barrel fold	325:339	This study reported simultaneously improved thermostability and hydrolytic pattern of α-amylase from Bacillus subtilis CN7 by rationally engineering the mostly conserved central beta strands in TIM barrel fold.
32219624	3	43	theme	active	573:578	arg1	mutants					580:586	All the five active mutants	560:586	All the five active mutants	560:586	All the five active mutants changed the compositions and percentages of maltooligosaccharides in final hydrolytic products compared to the product spectrum of the wild-type.
32219624	3	44	dep	compositions	600:611	arg1	the					596:598	the	596:598	the	596:598	All the five active mutants changed the compositions and percentages of maltooligosaccharides in final hydrolytic products compared to the product spectrum of the wild-type.
32219624	0	45	theme	Hydrolytic	44:53	arg1	Pattern					55:61	Hydrolytic Pattern	44:61	Hydrolytic Pattern	44:61	Simultaneously Improved Thermostability and Hydrolytic Pattern of Alpha-Amylase by Engineering Central Beta Strands of TIM Barrel.
32219624	5	46	theme	TIM	1172:1174	arg1	barrel					1176:1181	TIM barrel	1172:1181	TIM barrel	1172:1181	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	2	47	theme	strand	464:469	arg1	site					449:452	3rd site	445:452	3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase	445:557	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	2	47	theme	strand	464:469	arg1	site					419:422	the 2nd site	411:422	the 2nd site of the β7 strand	411:439	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	2	47	theme	strand	464:469	arg1	strand					464:469	the β5 strand	457:469	the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase	457:557	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	2	47	theme	strand	464:469	arg1	strand					434:439	the β7 strand	427:439	the β7 strand	427:439	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	2	48	from	interactions	495:506	arg1	strands					520:526	the beta strands	511:526	the beta strands of the TIM barrel of α-amylase	511:557	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	5	49	theme	first	1033:1037	arg1	this					1021:1024	this	1021:1024	this	1021:1024	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	5	49	theme	first	1033:1037	arg1	report					1039:1044	the first report	1029:1044	the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase	1029:1131	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	0	50	theme	Alpha-Amylase	66:78	arg1	Thermostability					24:38	Thermostability	24:38	Thermostability	24:38	Simultaneously Improved Thermostability and Hydrolytic Pattern of Alpha-Amylase by Engineering Central Beta Strands of TIM Barrel.
32219624	0	50	theme	Alpha-Amylase	66:78	arg1	Pattern					55:61	Hydrolytic Pattern	44:61	Hydrolytic Pattern	44:61	Simultaneously Improved Thermostability and Hydrolytic Pattern of Alpha-Amylase by Engineering Central Beta Strands of TIM Barrel.
32219624	5	51	theme	barrel	1176:1181	arg1	strands					1161:1167	central beta strands	1148:1167	central beta strands of TIM barrel	1148:1181	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	4	52	theme	maltooligosaccharide-producing	901:930	arg1	amylase					932:938	novel maltooligosaccharide-producing amylase	895:938	novel maltooligosaccharide-producing amylase	895:938	A mutant Y204V produced only maltose, maltotriose, and maltopentaose without any glucose and maltotetraose, indicating a conversion from typical endo-amylase to novel maltooligosaccharide-producing amylase.
32219624	2	53	theme	weak	490:493	arg1	interactions					495:506	the weak interactions	486:506	the weak interactions in the beta strands of the TIM barrel of α-amylase	486:557	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	1	54	from	CN7	250:252	arg1	thermostability					175:189	thermostability	175:189	thermostability	175:189	This study reported simultaneously improved thermostability and hydrolytic pattern of α-amylase from Bacillus subtilis CN7 by rationally engineering the mostly conserved central beta strands in TIM barrel fold.
32219624	1	54	from	CN7	250:252	arg1	pattern					206:212	hydrolytic pattern	195:212	hydrolytic pattern	195:212	This study reported simultaneously improved thermostability and hydrolytic pattern of α-amylase from Bacillus subtilis CN7 by rationally engineering the mostly conserved central beta strands in TIM barrel fold.
32219624	4	55	theme	novel	895:899	arg1	amylase					932:938	novel maltooligosaccharide-producing amylase	895:938	novel maltooligosaccharide-producing amylase	895:938	A mutant Y204V produced only maltose, maltotriose, and maltopentaose without any glucose and maltotetraose, indicating a conversion from typical endo-amylase to novel maltooligosaccharide-producing amylase.
32219624	5	56	theme	simultaneous	1053:1064	arg1	improvement					1066:1076	the simultaneous improvement	1049:1076	the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase	1049:1131	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	5	57	theme	hydrolytic	1101:1110	arg1	pattern					1112:1118	hydrolytic pattern	1101:1118	hydrolytic pattern	1101:1118	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	5	58	theme	other	1280:1284	arg1	proteins					1297:1304	other TIM barrel proteins	1280:1304	other TIM barrel proteins	1280:1304	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	2	59	theme	α-amylase	549:557	arg1	barrel					539:544	the TIM barrel	531:544	the TIM barrel of α-amylase	531:557	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	4	60	theme	mutant	736:741	arg1	Y204V					743:747	A mutant Y204V	734:747	A mutant Y204V	734:747	A mutant Y204V produced only maltose, maltotriose, and maltopentaose without any glucose and maltotetraose, indicating a conversion from typical endo-amylase to novel maltooligosaccharide-producing amylase.
32219624	5	61	theme	TIM	1286:1288	arg1	proteins					1297:1304	other TIM barrel proteins	1280:1304	other TIM barrel proteins	1280:1304	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	3	62	theme	final	657:661	arg1	products					674:681	final hydrolytic products	657:681	final hydrolytic products	657:681	All the five active mutants changed the compositions and percentages of maltooligosaccharides in final hydrolytic products compared to the product spectrum of the wild-type.
32219624	2	63	theme	point	354:358	arg1	mutations					360:368	Nine single point mutations	342:368	Nine single point mutations	342:368	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	5	64	theme	barrel	1290:1295	arg1	proteins					1297:1304	other TIM barrel proteins	1280:1304	other TIM barrel proteins	1280:1304	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	3	65	theme	hydrolytic	663:672	arg1	products					674:681	final hydrolytic products	657:681	final hydrolytic products	657:681	All the five active mutants changed the compositions and percentages of maltooligosaccharides in final hydrolytic products compared to the product spectrum of the wild-type.
32219624	5	66	theme	proteins	1297:1304	arg1	engineering					1265:1275	the protein engineering	1253:1275	the protein engineering of other TIM barrel proteins	1253:1304	A mutant V260I enhanced the thermal stability by 7.1 °C. To our best knowledge, this is the first report on the simultaneous improvement of thermostability and hydrolytic pattern of α-amylase by engineering central beta strands of TIM barrel and the novel "beta strands" strategy proposed here may be useful for the protein engineering of other TIM barrel proteins.
32219624	2	67	theme	β5	461:462	arg1	strand					464:469	the β5 strand	457:469	the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase	457:557	Nine single point mutations and a double mutation were introduced at the 2nd site of the β7 strand and 3rd site of the β5 strand to rationalize the weak interactions in the beta strands of the TIM barrel of α-amylase.
32219624	3	68	theme	product	699:705	arg1	spectrum					707:714	the product spectrum	695:714	the product spectrum of the wild-type	695:731	All the five active mutants changed the compositions and percentages of maltooligosaccharides in final hydrolytic products compared to the product spectrum of the wild-type.
32219624	1	69	dep	Bacillus	232:239	arg1	subtilis					241:248	subtilis	241:248	subtilis	241:248	This study reported simultaneously improved thermostability and hydrolytic pattern of α-amylase from Bacillus subtilis CN7 by rationally engineering the mostly conserved central beta strands in TIM barrel fold.
34968544	2	0	theme	environmental	648:660	arg1	friendliness					662:673	environmental friendliness	648:673	environmental friendliness	648:673	The SA/PL fiber exhibits good antibacterial activity, low cytotoxicity, anti-hemolysis, bioadhesion, and environmental friendliness due to its natural raw materials and green preparation process.
34968544	1	1	theme	filamentou	170:179	arg1	fiber					221:225	a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber	162:225	a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes	162:285	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	3	2	theme	inflammation	913:924	arg1	reduction					900:908	reduction	900:908	reduction of inflammation	900:924	In vivo experiments have shown that the SA/PL fiber can promote the healing and repair of skin wounds on the backs of mice via resistance to pathogen infection, reduction of inflammation, and anti-allogeneic allergy of the wound.
34968544	3	2	theme	inflammation	913:924	arg1	infection					889:897	pathogen infection	880:897	pathogen infection	880:897	In vivo experiments have shown that the SA/PL fiber can promote the healing and repair of skin wounds on the backs of mice via resistance to pathogen infection, reduction of inflammation, and anti-allogeneic allergy of the wound.
34968544	3	2	theme	inflammation	913:924	arg1	allergy					947:953	anti-allogeneic allergy	931:953	anti-allogeneic allergy of the wound	931:966	In vivo experiments have shown that the SA/PL fiber can promote the healing and repair of skin wounds on the backs of mice via resistance to pathogen infection, reduction of inflammation, and anti-allogeneic allergy of the wound.
34968544	3	3	theme	mice	857:860	arg1	backs					848:852	the backs	844:852	the backs of mice	844:860	In vivo experiments have shown that the SA/PL fiber can promote the healing and repair of skin wounds on the backs of mice via resistance to pathogen infection, reduction of inflammation, and anti-allogeneic allergy of the wound.
34968544	1	4	from	interface	440:448	arg1	pulling					366:372	continuous pulling	355:372	continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA	355:540	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	1	4	from	interface	440:448	arg1	composites					414:423	an electrostatically assembled SA/PL composites	377:423	an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA	377:540	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	1	5	theme	continuous	355:364	arg1	pulling					366:372	continuous pulling	355:372	continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA	355:540	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	1	6	theme	solutions	461:469	arg1	interface					440:448	the contact interface	428:448	the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA	428:540	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	1	7	theme	sodium	181:186	arg1	fiber					221:225	a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber	162:225	a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes	162:285	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	3	8	from	healing	807:813	arg1	backs					848:852	the backs	844:852	the backs of mice	844:860	In vivo experiments have shown that the SA/PL fiber can promote the healing and repair of skin wounds on the backs of mice via resistance to pathogen infection, reduction of inflammation, and anti-allogeneic allergy of the wound.
34968544	2	9	theme	good	568:571	arg1	activity					587:594	good antibacterial activity	568:594	good antibacterial activity	568:594	The SA/PL fiber exhibits good antibacterial activity, low cytotoxicity, anti-hemolysis, bioadhesion, and environmental friendliness due to its natural raw materials and green preparation process.
34968544	3	10	from	repair	819:824	arg1	backs					848:852	the backs	844:852	the backs of mice	844:860	In vivo experiments have shown that the SA/PL fiber can promote the healing and repair of skin wounds on the backs of mice via resistance to pathogen infection, reduction of inflammation, and anti-allogeneic allergy of the wound.
34968544	2	11	theme	raw	694:696	arg1	materials					698:706	its natural raw materials	682:706	its natural raw materials	682:706	The SA/PL fiber exhibits good antibacterial activity, low cytotoxicity, anti-hemolysis, bioadhesion, and environmental friendliness due to its natural raw materials and green preparation process.
34968544	1	12	theme	controllable	268:279	arg1	sizes					281:285	controllable sizes	268:285	controllable sizes	268:285	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	3	13	dep	healing	807:813	arg1	the					803:805	the	803:805	the	803:805	In vivo experiments have shown that the SA/PL fiber can promote the healing and repair of skin wounds on the backs of mice via resistance to pathogen infection, reduction of inflammation, and anti-allogeneic allergy of the wound.
34968544	1	14	theme	cationic	474:481	arg1	ε-PL					499:502	cationic polyelectrolyte ε-PL	474:502	cationic polyelectrolyte ε-PL	474:502	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	3	15	theme	anti-allogeneic	931:945	arg1	allergy					947:953	anti-allogeneic allergy	931:953	anti-allogeneic allergy of the wound	931:966	In vivo experiments have shown that the SA/PL fiber can promote the healing and repair of skin wounds on the backs of mice via resistance to pathogen infection, reduction of inflammation, and anti-allogeneic allergy of the wound.
34968544	3	16	theme	SA/PL	779:783	arg1	fiber					785:789	the SA/PL fiber	775:789	the SA/PL fiber	775:789	In vivo experiments have shown that the SA/PL fiber can promote the healing and repair of skin wounds on the backs of mice via resistance to pathogen infection, reduction of inflammation, and anti-allogeneic allergy of the wound.
34968544	1	17	theme	polyelectrolyte	483:497	arg1	ε-PL					499:502	cationic polyelectrolyte ε-PL	474:502	cationic polyelectrolyte ε-PL	474:502	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	1	18	theme	alginate	188:195	arg1	fiber					221:225	a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber	162:225	a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes	162:285	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	2	19	theme	SA/PL	547:551	arg1	fiber					553:557	The SA/PL fiber	543:557	The SA/PL fiber	543:557	The SA/PL fiber exhibits good antibacterial activity, low cytotoxicity, anti-hemolysis, bioadhesion, and environmental friendliness due to its natural raw materials and green preparation process.
34968544	2	20	theme	green	712:716	arg1	process					730:736	green preparation process	712:736	green preparation process	712:736	The SA/PL fiber exhibits good antibacterial activity, low cytotoxicity, anti-hemolysis, bioadhesion, and environmental friendliness due to its natural raw materials and green preparation process.
34968544	1	21	theme	ε-PL	499:502	arg1	solutions					461:469	aqueous solutions	453:469	aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA	453:540	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	4	22	theme	green	1033:1037	arg1	material					1083:1090	a green and biosafe multifunctional natural polymer material	1031:1090	a green and biosafe multifunctional natural polymer material	1031:1090	In summary, these results demonstrate that the SA/PL fiber is a green and biosafe multifunctional natural polymer material, with potential applications in suturing wound.
34968544	4	22	theme	green	1033:1037	arg1	fiber					1022:1026	the SA/PL fiber	1012:1026	the SA/PL fiber	1012:1026	In summary, these results demonstrate that the SA/PL fiber is a green and biosafe multifunctional natural polymer material, with potential applications in suturing wound.
34968544	1	23	theme	assembled	398:406	arg1	composites					414:423	an electrostatically assembled SA/PL composites	377:423	an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA	377:540	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	2	24	theme	due	675:677	arg1	activity					587:594	good antibacterial activity	568:594	good antibacterial activity	568:594	The SA/PL fiber exhibits good antibacterial activity, low cytotoxicity, anti-hemolysis, bioadhesion, and environmental friendliness due to its natural raw materials and green preparation process.
34968544	1	25	theme	SA	198:199	arg1	fiber					221:225	a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber	162:225	a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes	162:285	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	3	26	theme	In	739:740	arg1	experiments					747:757	In vivo experiments	739:757	In vivo experiments	739:757	In vivo experiments have shown that the SA/PL fiber can promote the healing and repair of skin wounds on the backs of mice via resistance to pathogen infection, reduction of inflammation, and anti-allogeneic allergy of the wound.
34968544	3	27	dep	In	739:740	arg1	vivo					742:745	vivo	742:745	vivo	742:745	In vivo experiments have shown that the SA/PL fiber can promote the healing and repair of skin wounds on the backs of mice via resistance to pathogen infection, reduction of inflammation, and anti-allogeneic allergy of the wound.
34968544	1	28	theme	anionic	508:514	arg1	SA					539:540	anionic natural polysaccharide SA	508:540	anionic natural polysaccharide SA	508:540	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	3	29	theme	wound	962:966	arg1	reduction					900:908	reduction	900:908	reduction of inflammation	900:924	In vivo experiments have shown that the SA/PL fiber can promote the healing and repair of skin wounds on the backs of mice via resistance to pathogen infection, reduction of inflammation, and anti-allogeneic allergy of the wound.
34968544	3	29	theme	wound	962:966	arg1	infection					889:897	pathogen infection	880:897	pathogen infection	880:897	In vivo experiments have shown that the SA/PL fiber can promote the healing and repair of skin wounds on the backs of mice via resistance to pathogen infection, reduction of inflammation, and anti-allogeneic allergy of the wound.
34968544	3	29	theme	wound	962:966	arg1	allergy					947:953	anti-allogeneic allergy	931:953	anti-allogeneic allergy of the wound	931:966	In vivo experiments have shown that the SA/PL fiber can promote the healing and repair of skin wounds on the backs of mice via resistance to pathogen infection, reduction of inflammation, and anti-allogeneic allergy of the wound.
34968544	0	30	theme	sodium	45:50	arg1	fiber					74:78	filamentous sodium alginate/ε-polylysine fiber	33:78	filamentous sodium alginate/ε-polylysine fiber	33:78	Electrostatically self-assembled filamentous sodium alginate/ε-polylysine fiber with antibacterial, bioadhesion and biocompatible in suturing wound.
34968544	2	31	theme	preparation	718:728	arg1	process					730:736	green preparation process	712:736	green preparation process	712:736	The SA/PL fiber exhibits good antibacterial activity, low cytotoxicity, anti-hemolysis, bioadhesion, and environmental friendliness due to its natural raw materials and green preparation process.
34968544	2	32	theme	low	597:599	arg1	cytotoxicity					601:612	low cytotoxicity	597:612	low cytotoxicity	597:612	The SA/PL fiber exhibits good antibacterial activity, low cytotoxicity, anti-hemolysis, bioadhesion, and environmental friendliness due to its natural raw materials and green preparation process.
34968544	1	33	theme	natural	516:522	arg1	SA					539:540	anionic natural polysaccharide SA	508:540	anionic natural polysaccharide SA	508:540	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	2	34	theme	natural	686:692	arg1	materials					698:706	its natural raw materials	682:706	its natural raw materials	682:706	The SA/PL fiber exhibits good antibacterial activity, low cytotoxicity, anti-hemolysis, bioadhesion, and environmental friendliness due to its natural raw materials and green preparation process.
34968544	1	35	theme	/ε-polylysine	202:214	arg1	fiber					221:225	a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber	162:225	a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes	162:285	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	0	36	theme	filamentous	33:43	arg1	fiber					74:78	filamentous sodium alginate/ε-polylysine fiber	33:78	filamentous sodium alginate/ε-polylysine fiber	33:78	Electrostatically self-assembled filamentous sodium alginate/ε-polylysine fiber with antibacterial, bioadhesion and biocompatible in suturing wound.
34968544	1	37	theme	SA/PL	408:412	arg1	composites					414:423	an electrostatically assembled SA/PL composites	377:423	an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA	377:540	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	1	38	theme	composites	414:423	arg1	pulling					366:372	continuous pulling	355:372	continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA	355:540	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	4	39	theme	multifunctional	1051:1065	arg1	material					1083:1090	a green and biosafe multifunctional natural polymer material	1031:1090	a green and biosafe multifunctional natural polymer material	1031:1090	In summary, these results demonstrate that the SA/PL fiber is a green and biosafe multifunctional natural polymer material, with potential applications in suturing wound.
34968544	4	39	theme	multifunctional	1051:1065	arg1	fiber					1022:1026	the SA/PL fiber	1012:1026	the SA/PL fiber	1012:1026	In summary, these results demonstrate that the SA/PL fiber is a green and biosafe multifunctional natural polymer material, with potential applications in suturing wound.
34968544	1	40	theme	PL	217:218	arg1	fiber					221:225	a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber	162:225	a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes	162:285	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	0	41	theme	alginate/ε-polylysine	52:72	arg1	fiber					74:78	filamentous sodium alginate/ε-polylysine fiber	33:78	filamentous sodium alginate/ε-polylysine fiber	33:78	Electrostatically self-assembled filamentous sodium alginate/ε-polylysine fiber with antibacterial, bioadhesion and biocompatible in suturing wound.
34968544	1	42	theme	efficient	305:313	arg1	manner					344:349	an efficient and environmentally friendly manner	302:349	an efficient and environmentally friendly manner	302:349	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	1	43	from	pulling	366:372	arg1	interface					440:448	the contact interface	428:448	the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA	428:540	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	3	44	theme	skin	829:832	arg1	wounds					834:839	skin wounds	829:839	skin wounds	829:839	In vivo experiments have shown that the SA/PL fiber can promote the healing and repair of skin wounds on the backs of mice via resistance to pathogen infection, reduction of inflammation, and anti-allogeneic allergy of the wound.
34968544	1	45	theme	polysaccharide	524:537	arg1	SA					539:540	anionic natural polysaccharide SA	508:540	anionic natural polysaccharide SA	508:540	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	1	46	theme	SA	539:540	arg1	solutions					461:469	aqueous solutions	453:469	aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA	453:540	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	2	47	theme	antibacterial	573:585	arg1	activity					587:594	good antibacterial activity	568:594	good antibacterial activity	568:594	The SA/PL fiber exhibits good antibacterial activity, low cytotoxicity, anti-hemolysis, bioadhesion, and environmental friendliness due to its natural raw materials and green preparation process.
34968544	1	48	theme	environmentally	319:333	arg1	manner					344:349	an efficient and environmentally friendly manner	302:349	an efficient and environmentally friendly manner	302:349	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	1	49	theme	contact	432:438	arg1	interface					440:448	the contact interface	428:448	the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA	428:540	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	4	50	with	material	1083:1090	arg1	applications					1108:1119	potential applications	1098:1119	potential applications in suturing wound	1098:1137	In summary, these results demonstrate that the SA/PL fiber is a green and biosafe multifunctional natural polymer material, with potential applications in suturing wound.
34968544	3	51	theme	pathogen	880:887	arg1	infection					889:897	pathogen infection	880:897	pathogen infection	880:897	In vivo experiments have shown that the SA/PL fiber can promote the healing and repair of skin wounds on the backs of mice via resistance to pathogen infection, reduction of inflammation, and anti-allogeneic allergy of the wound.
34968544	1	52	theme	friendly	335:342	arg1	manner					344:349	an efficient and environmentally friendly manner	302:349	an efficient and environmentally friendly manner	302:349	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	4	53	theme	biosafe	1043:1049	arg1	material					1083:1090	a green and biosafe multifunctional natural polymer material	1031:1090	a green and biosafe multifunctional natural polymer material	1031:1090	In summary, these results demonstrate that the SA/PL fiber is a green and biosafe multifunctional natural polymer material, with potential applications in suturing wound.
34968544	4	53	theme	biosafe	1043:1049	arg1	fiber					1022:1026	the SA/PL fiber	1012:1026	the SA/PL fiber	1012:1026	In summary, these results demonstrate that the SA/PL fiber is a green and biosafe multifunctional natural polymer material, with potential applications in suturing wound.
34968544	4	54	theme	SA/PL	1016:1020	arg1	material					1083:1090	a green and biosafe multifunctional natural polymer material	1031:1090	a green and biosafe multifunctional natural polymer material	1031:1090	In summary, these results demonstrate that the SA/PL fiber is a green and biosafe multifunctional natural polymer material, with potential applications in suturing wound.
34968544	4	54	theme	SA/PL	1016:1020	arg1	fiber					1022:1026	the SA/PL fiber	1012:1026	the SA/PL fiber	1012:1026	In summary, these results demonstrate that the SA/PL fiber is a green and biosafe multifunctional natural polymer material, with potential applications in suturing wound.
34968544	4	55	theme	potential	1098:1106	arg1	applications					1108:1119	potential applications	1098:1119	potential applications in suturing wound	1098:1137	In summary, these results demonstrate that the SA/PL fiber is a green and biosafe multifunctional natural polymer material, with potential applications in suturing wound.
34968544	4	56	theme	natural	1067:1073	arg1	material					1083:1090	a green and biosafe multifunctional natural polymer material	1031:1090	a green and biosafe multifunctional natural polymer material	1031:1090	In summary, these results demonstrate that the SA/PL fiber is a green and biosafe multifunctional natural polymer material, with potential applications in suturing wound.
34968544	4	56	theme	natural	1067:1073	arg1	fiber					1022:1026	the SA/PL fiber	1012:1026	the SA/PL fiber	1012:1026	In summary, these results demonstrate that the SA/PL fiber is a green and biosafe multifunctional natural polymer material, with potential applications in suturing wound.
34968544	1	57	theme	aqueous	453:459	arg1	solutions					461:469	aqueous solutions	453:469	aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA	453:540	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	1	58	theme	excellent	232:240	arg1	properties					253:262	excellent mechanical properties	232:262	excellent mechanical properties	232:262	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	3	59	theme	wounds	834:839	arg1	repair					819:824	repair	819:824	repair	819:824	In vivo experiments have shown that the SA/PL fiber can promote the healing and repair of skin wounds on the backs of mice via resistance to pathogen infection, reduction of inflammation, and anti-allogeneic allergy of the wound.
34968544	3	59	theme	wounds	834:839	arg1	healing					807:813	healing	807:813	healing	807:813	In vivo experiments have shown that the SA/PL fiber can promote the healing and repair of skin wounds on the backs of mice via resistance to pathogen infection, reduction of inflammation, and anti-allogeneic allergy of the wound.
34968544	1	60	with	fiber	221:225	arg1	properties					253:262	excellent mechanical properties	232:262	excellent mechanical properties	232:262	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	1	60	with	fiber	221:225	arg1	sizes					281:285	controllable sizes	268:285	controllable sizes	268:285	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	1	61	theme	novel	164:168	arg1	fiber					221:225	a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber	162:225	a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes	162:285	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34968544	4	62	theme	polymer	1075:1081	arg1	material					1083:1090	a green and biosafe multifunctional natural polymer material	1031:1090	a green and biosafe multifunctional natural polymer material	1031:1090	In summary, these results demonstrate that the SA/PL fiber is a green and biosafe multifunctional natural polymer material, with potential applications in suturing wound.
34968544	4	62	theme	polymer	1075:1081	arg1	fiber					1022:1026	the SA/PL fiber	1012:1026	the SA/PL fiber	1012:1026	In summary, these results demonstrate that the SA/PL fiber is a green and biosafe multifunctional natural polymer material, with potential applications in suturing wound.
34968544	1	63	theme	mechanical	242:251	arg1	properties					253:262	excellent mechanical properties	232:262	excellent mechanical properties	232:262	In the work, a novel filamentou sodium alginate (SA) /ε-polylysine (PL) fiber with excellent mechanical properties and controllable sizes is prepared in an efficient and environmentally friendly manner via continuous pulling of an electrostatically assembled SA/PL composites at the contact interface of aqueous solutions of cationic polyelectrolyte ε-PL and anionic natural polysaccharide SA.
34894157	5	0	theme	intermolecular	782:795	arg1	aggregation					797:807	side-by-side intermolecular aggregation	769:807	side-by-side intermolecular aggregation of KC helices	769:821	Further investigation revealed that 4 g kg-1 CMC promoted side-by-side intermolecular aggregation of KC helices through hydrogen bonding, which stabilized a denser network structure compared to the pure KC hydrogel.
34894157	4	1	theme	water	681:685	arg1	seepage					687:693	limited water seepage	673:693	limited water seepage	673:693	The results showed that 4 g kg-1 carboxymethylcellulose (CMC) composited with 20 g kg-1 KC formed a flexible gummy candy with low fragility and limited water seepage during storage.
34894157	3	2	from	effects	435:441	arg1	properties					475:484	texture properties	467:484	texture properties	467:484	RESULTS This study evaluated the effects of hydrocolloids on the texture properties and storage stability of KC gummy candies.
34894157	3	2	from	effects	435:441	arg1	stability					498:506	storage stability	490:506	storage stability	490:506	RESULTS This study evaluated the effects of hydrocolloids on the texture properties and storage stability of KC gummy candies.
34894157	7	3	theme	KC	1260:1261	arg1	candies					1269:1275	KC gummy candies	1260:1275	KC gummy candies	1260:1275	CONCLUSION This study found that 4 g kg-1 CMC was able to improve the flexibility and decrease unacceptable fragility of KC gummy candies, with water seepage decreased during storage significantly.
34894157	6	4	theme	high	936:939	arg1	8-12 g kg-1					958:968	8-12 g kg-1	958:968	8-12 g kg-1	958:968	However, high CMC proportions (8-12 g kg-1 ) led to electrostatic repulsion that dominated in the system, inhibiting the gel-forming process and thus resulting in a weak gel structure with accelerated syneresis.
34894157	6	4	theme	high	936:939	arg1	proportions					945:955	high CMC proportions	936:955	high CMC proportions (8-12 g kg-1 )	936:970	However, high CMC proportions (8-12 g kg-1 ) led to electrostatic repulsion that dominated in the system, inhibiting the gel-forming process and thus resulting in a weak gel structure with accelerated syneresis.
34894157	6	5	theme	gel	1097:1099	arg1	structure					1101:1109	a weak gel structure	1090:1109	a weak gel structure with accelerated syneresis	1090:1136	However, high CMC proportions (8-12 g kg-1 ) led to electrostatic repulsion that dominated in the system, inhibiting the gel-forming process and thus resulting in a weak gel structure with accelerated syneresis.
34894157	5	6	theme	KC	914:915	arg1	hydrogel					917:924	the pure KC hydrogel	905:924	the pure KC hydrogel	905:924	Further investigation revealed that 4 g kg-1 CMC promoted side-by-side intermolecular aggregation of KC helices through hydrogen bonding, which stabilized a denser network structure compared to the pure KC hydrogel.
34894157	0	7	theme	vegan	96:100	arg1	gummy					102:106	vegan gummy	96:106	vegan gummy	96:106	The improvement of texture properties and storage stability for kappa carrageenan in developing vegan gummy candies.
34894157	8	8	theme	KC	1447:1448	arg1	gels					1450:1453	KC gels	1447:1453	KC gels	1447:1453	It provided preliminary evidence for utilizing hydrocolloids to adjust texture and control water migration in KC gels, and has potential to promote wide development of vegan gummy candies.
34894157	2	9	theme	texture	290:296	arg1	defects					298:304	texture defects	290:304	texture defects	290:304	However, texture defects and water seepage during storage limit the development of kappa carrageenan (KC) gummy candies.
34894157	4	10	theme	low	655:657	arg1	fragility					659:667	low fragility	655:667	low fragility	655:667	The results showed that 4 g kg-1 carboxymethylcellulose (CMC) composited with 20 g kg-1 KC formed a flexible gummy candy with low fragility and limited water seepage during storage.
34894157	1	11	theme	confectionery	257:269	arg1	products					271:278	confectionery products	257:278	confectionery products	257:278	BACKGROUND As plant-based foods have become more mainstream in recent years, carrageenan has been used to replace animal-derived gelatin in confectionery products.
34894157	6	12	with	structure	1101:1109	arg1	syneresis					1128:1136	accelerated syneresis	1116:1136	accelerated syneresis	1116:1136	However, high CMC proportions (8-12 g kg-1 ) led to electrostatic repulsion that dominated in the system, inhibiting the gel-forming process and thus resulting in a weak gel structure with accelerated syneresis.
34894157	7	13	theme	unacceptable	1234:1245	arg1	fragility					1247:1255	unacceptable fragility	1234:1255	unacceptable fragility of KC gummy candies	1234:1275	CONCLUSION This study found that 4 g kg-1 CMC was able to improve the flexibility and decrease unacceptable fragility of KC gummy candies, with water seepage decreased during storage significantly.
34894157	3	14	dep	properties	475:484	arg1	the					463:465	the	463:465	the	463:465	RESULTS This study evaluated the effects of hydrocolloids on the texture properties and storage stability of KC gummy candies.
34894157	7	15	theme	water	1283:1287	arg1	seepage					1289:1295	water seepage	1283:1295	water seepage	1283:1295	CONCLUSION This study found that 4 g kg-1 CMC was able to improve the flexibility and decrease unacceptable fragility of KC gummy candies, with water seepage decreased during storage significantly.
34894157	5	16	theme	4 g kg-1	747:754	arg1	CMC					756:758	4 g kg-1 CMC	747:758	4 g kg-1 CMC	747:758	Further investigation revealed that 4 g kg-1 CMC promoted side-by-side intermolecular aggregation of KC helices through hydrogen bonding, which stabilized a denser network structure compared to the pure KC hydrogel.
34894157	6	17	theme	weak	1092:1095	arg1	structure					1101:1109	a weak gel structure	1090:1109	a weak gel structure with accelerated syneresis	1090:1136	However, high CMC proportions (8-12 g kg-1 ) led to electrostatic repulsion that dominated in the system, inhibiting the gel-forming process and thus resulting in a weak gel structure with accelerated syneresis.
34894157	3	18	theme	candies	520:526	arg1	properties					475:484	texture properties	467:484	texture properties	467:484	RESULTS This study evaluated the effects of hydrocolloids on the texture properties and storage stability of KC gummy candies.
34894157	3	18	theme	candies	520:526	arg1	stability					498:506	storage stability	490:506	storage stability	490:506	RESULTS This study evaluated the effects of hydrocolloids on the texture properties and storage stability of KC gummy candies.
34894157	1	19	theme	recent	180:185	arg1	years					187:191	recent years	180:191	recent years	180:191	BACKGROUND As plant-based foods have become more mainstream in recent years, carrageenan has been used to replace animal-derived gelatin in confectionery products.
34894157	2	20	theme	gummy	387:391	arg1	candies					393:399	kappa carrageenan (KC) gummy candies	364:399	kappa carrageenan (KC) gummy candies	364:399	However, texture defects and water seepage during storage limit the development of kappa carrageenan (KC) gummy candies.
34894157	6	21	theme	accelerated	1116:1126	arg1	syneresis					1128:1136	accelerated syneresis	1116:1136	accelerated syneresis	1116:1136	However, high CMC proportions (8-12 g kg-1 ) led to electrostatic repulsion that dominated in the system, inhibiting the gel-forming process and thus resulting in a weak gel structure with accelerated syneresis.
34894157	8	22	from	migration	1434:1442	arg1	gels					1450:1453	KC gels	1447:1453	KC gels	1447:1453	It provided preliminary evidence for utilizing hydrocolloids to adjust texture and control water migration in KC gels, and has potential to promote wide development of vegan gummy candies.
34894157	5	23	theme	hydrogen	831:838	arg1	bonding					840:846	hydrogen bonding	831:846	hydrogen bonding	831:846	Further investigation revealed that 4 g kg-1 CMC promoted side-by-side intermolecular aggregation of KC helices through hydrogen bonding, which stabilized a denser network structure compared to the pure KC hydrogel.
34894157	3	24	theme	gummy	514:518	arg1	candies					520:526	KC gummy candies	511:526	KC gummy candies	511:526	RESULTS This study evaluated the effects of hydrocolloids on the texture properties and storage stability of KC gummy candies.
34894157	3	25	dep	RESULTS	402:408	arg1	evaluated					421:429	evaluated	421:429	evaluated the effects of hydrocolloids on the texture properties and storage stability of KC gummy candies	421:526	RESULTS This study evaluated the effects of hydrocolloids on the texture properties and storage stability of KC gummy candies.
34894157	1	26	link	animal-derived	231:244	arg1	gelatin					246:252	animal-derived gelatin	231:252	animal-derived gelatin	231:252	BACKGROUND As plant-based foods have become more mainstream in recent years, carrageenan has been used to replace animal-derived gelatin in confectionery products.
34894157	9	27	theme	Chemical	1544:1551	arg1	Industry					1553:1560	Chemical Industry	1544:1560	Chemical Industry	1544:1560	© 2021 Society of Chemical Industry.
34894157	2	28	theme	KC	383:384	arg1	candies					393:399	kappa carrageenan (KC) gummy candies	364:399	kappa carrageenan (KC) gummy candies	364:399	However, texture defects and water seepage during storage limit the development of kappa carrageenan (KC) gummy candies.
34894157	7	29	theme	4 g kg-1	1172:1179	arg1	CMC					1181:1183	4 g kg-1 CMC	1172:1183	4 g kg-1 CMC	1172:1183	CONCLUSION This study found that 4 g kg-1 CMC was able to improve the flexibility and decrease unacceptable fragility of KC gummy candies, with water seepage decreased during storage significantly.
34894157	8	30	theme	vegan	1505:1509	arg1	candies					1517:1523	vegan gummy candies	1505:1523	vegan gummy candies	1505:1523	It provided preliminary evidence for utilizing hydrocolloids to adjust texture and control water migration in KC gels, and has potential to promote wide development of vegan gummy candies.
34894157	2	31	theme	water	310:314	arg1	seepage					316:322	water seepage	310:322	water seepage during storage	310:337	However, texture defects and water seepage during storage limit the development of kappa carrageenan (KC) gummy candies.
34894157	0	32	theme	texture	19:25	arg1	properties					27:36	texture properties	19:36	texture properties	19:36	The improvement of texture properties and storage stability for kappa carrageenan in developing vegan gummy candies.
34894157	6	33	theme	electrostatic	979:991	arg1	repulsion					993:1001	electrostatic repulsion	979:1001	electrostatic repulsion that dominated in the system, inhibiting the gel-forming process and thus resulting in a weak gel structure with accelerated syneresis	979:1136	However, high CMC proportions (8-12 g kg-1 ) led to electrostatic repulsion that dominated in the system, inhibiting the gel-forming process and thus resulting in a weak gel structure with accelerated syneresis.
34894157	8	34	theme	preliminary	1349:1359	arg1	evidence					1361:1368	preliminary evidence	1349:1368	preliminary evidence for utilizing hydrocolloids to adjust texture and control water migration in KC gels, and has potential to promote wide development of vegan gummy candies	1349:1523	It provided preliminary evidence for utilizing hydrocolloids to adjust texture and control water migration in KC gels, and has potential to promote wide development of vegan gummy candies.
34894157	4	35	theme	flexible	629:636	arg1	candy					644:648	a flexible gummy candy	627:648	a flexible gummy candy	627:648	The results showed that 4 g kg-1 carboxymethylcellulose (CMC) composited with 20 g kg-1 KC formed a flexible gummy candy with low fragility and limited water seepage during storage.
34894157	8	36	theme	water	1428:1432	arg1	migration					1434:1442	water migration	1428:1442	water migration in KC gels	1428:1453	It provided preliminary evidence for utilizing hydrocolloids to adjust texture and control water migration in KC gels, and has potential to promote wide development of vegan gummy candies.
34894157	7	37	theme	gummy	1263:1267	arg1	candies					1269:1275	KC gummy candies	1260:1275	KC gummy candies	1260:1275	CONCLUSION This study found that 4 g kg-1 CMC was able to improve the flexibility and decrease unacceptable fragility of KC gummy candies, with water seepage decreased during storage significantly.
34894157	3	38	theme	KC	511:512	arg1	candies					520:526	KC gummy candies	511:526	KC gummy candies	511:526	RESULTS This study evaluated the effects of hydrocolloids on the texture properties and storage stability of KC gummy candies.
34894157	4	39	theme	4 g kg-1	553:560	arg1	CMC					586:588	CMC	586:588	CMC	586:588	The results showed that 4 g kg-1 carboxymethylcellulose (CMC) composited with 20 g kg-1 KC formed a flexible gummy candy with low fragility and limited water seepage during storage.
34894157	4	39	theme	4 g kg-1	553:560	arg1	carboxymethylcellulose					562:583	4 g kg-1 carboxymethylcellulose	553:583	4 g kg-1 carboxymethylcellulose (CMC) composited with 20 g kg-1 KC	553:618	The results showed that 4 g kg-1 carboxymethylcellulose (CMC) composited with 20 g kg-1 KC formed a flexible gummy candy with low fragility and limited water seepage during storage.
34894157	5	40	theme	Further	711:717	arg1	investigation					719:731	Further investigation	711:731	Further investigation	711:731	Further investigation revealed that 4 g kg-1 CMC promoted side-by-side intermolecular aggregation of KC helices through hydrogen bonding, which stabilized a denser network structure compared to the pure KC hydrogel.
34894157	7	41	theme	candies	1269:1275	arg1	fragility					1247:1255	unacceptable fragility	1234:1255	unacceptable fragility of KC gummy candies	1234:1275	CONCLUSION This study found that 4 g kg-1 CMC was able to improve the flexibility and decrease unacceptable fragility of KC gummy candies, with water seepage decreased during storage significantly.
34894157	4	42	theme	gummy	638:642	arg1	candy					644:648	a flexible gummy candy	627:648	a flexible gummy candy	627:648	The results showed that 4 g kg-1 carboxymethylcellulose (CMC) composited with 20 g kg-1 KC formed a flexible gummy candy with low fragility and limited water seepage during storage.
34894157	5	43	theme	denser	868:873	arg1	structure					883:891	a denser network structure	866:891	a denser network structure	866:891	Further investigation revealed that 4 g kg-1 CMC promoted side-by-side intermolecular aggregation of KC helices through hydrogen bonding, which stabilized a denser network structure compared to the pure KC hydrogel.
34894157	0	44	theme	properties	27:36	arg1	improvement					4:14	The improvement	0:14	The improvement of texture properties and storage stability for kappa carrageenan in developing vegan gummy	0:106	The improvement of texture properties and storage stability for kappa carrageenan in developing vegan gummy candies.
34894157	5	45	theme	KC	812:813	arg1	helices					815:821	KC helices	812:821	KC helices	812:821	Further investigation revealed that 4 g kg-1 CMC promoted side-by-side intermolecular aggregation of KC helices through hydrogen bonding, which stabilized a denser network structure compared to the pure KC hydrogel.
34894157	8	46	theme	wide	1485:1488	arg1	development					1490:1500	wide development	1485:1500	wide development of vegan gummy candies	1485:1523	It provided preliminary evidence for utilizing hydrocolloids to adjust texture and control water migration in KC gels, and has potential to promote wide development of vegan gummy candies.
34894157	7	47	dep	CONCLUSION	1139:1148	arg1	found					1161:1165	found	1161:1165	found that 4 g kg-1 CMC was able to improve the flexibility and decrease unacceptable fragility of KC gummy candies, with water seepage decreased during storage significantly	1161:1334	CONCLUSION This study found that 4 g kg-1 CMC was able to improve the flexibility and decrease unacceptable fragility of KC gummy candies, with water seepage decreased during storage significantly.
34894157	5	48	theme	network	875:881	arg1	structure					883:891	a denser network structure	866:891	a denser network structure	866:891	Further investigation revealed that 4 g kg-1 CMC promoted side-by-side intermolecular aggregation of KC helices through hydrogen bonding, which stabilized a denser network structure compared to the pure KC hydrogel.
34894157	2	49	theme	candies	393:399	arg1	development					349:359	the development	345:359	the development of kappa carrageenan (KC) gummy candies	345:399	However, texture defects and water seepage during storage limit the development of kappa carrageenan (KC) gummy candies.
34894157	0	50	theme	stability	50:58	arg1	improvement					4:14	The improvement	0:14	The improvement of texture properties and storage stability for kappa carrageenan in developing vegan gummy	0:106	The improvement of texture properties and storage stability for kappa carrageenan in developing vegan gummy candies.
34894157	5	51	theme	pure	909:912	arg1	hydrogel					917:924	the pure KC hydrogel	905:924	the pure KC hydrogel	905:924	Further investigation revealed that 4 g kg-1 CMC promoted side-by-side intermolecular aggregation of KC helices through hydrogen bonding, which stabilized a denser network structure compared to the pure KC hydrogel.
34894157	6	52	theme	gel-forming	1048:1058	arg1	process					1060:1066	the gel-forming process	1044:1066	the gel-forming process	1044:1066	However, high CMC proportions (8-12 g kg-1 ) led to electrostatic repulsion that dominated in the system, inhibiting the gel-forming process and thus resulting in a weak gel structure with accelerated syneresis.
34894157	5	53	theme	side-by-side	769:780	arg1	aggregation					797:807	side-by-side intermolecular aggregation	769:807	side-by-side intermolecular aggregation of KC helices	769:821	Further investigation revealed that 4 g kg-1 CMC promoted side-by-side intermolecular aggregation of KC helices through hydrogen bonding, which stabilized a denser network structure compared to the pure KC hydrogel.
34894157	1	54	dep	mainstream	166:175	arg1	carrageenan					194:204	carrageenan	194:204	carrageenan	194:204	BACKGROUND As plant-based foods have become more mainstream in recent years, carrageenan has been used to replace animal-derived gelatin in confectionery products.
34894157	3	55	theme	hydrocolloids	446:458	arg1	effects					435:441	the effects	431:441	the effects of hydrocolloids on the texture properties and storage stability of KC gummy candies	431:526	RESULTS This study evaluated the effects of hydrocolloids on the texture properties and storage stability of KC gummy candies.
34894157	0	56	theme	storage	42:48	arg1	stability					50:58	storage stability	42:58	storage stability	42:58	The improvement of texture properties and storage stability for kappa carrageenan in developing vegan gummy candies.
34894157	8	57	dep	utilizing	1374:1382	arg1	control					1420:1426	control	1420:1426	control water migration in KC gels	1420:1453	It provided preliminary evidence for utilizing hydrocolloids to adjust texture and control water migration in KC gels, and has potential to promote wide development of vegan gummy candies.
34894157	8	57	dep	utilizing	1374:1382	arg1	adjust					1401:1406	adjust	1401:1406	adjust texture	1401:1414	It provided preliminary evidence for utilizing hydrocolloids to adjust texture and control water migration in KC gels, and has potential to promote wide development of vegan gummy candies.
34894157	8	57	dep	utilizing	1374:1382	arg1	has					1460:1462	has	1460:1462	has potential to promote wide development of vegan gummy candies	1460:1523	It provided preliminary evidence for utilizing hydrocolloids to adjust texture and control water migration in KC gels, and has potential to promote wide development of vegan gummy candies.
34894157	3	58	theme	storage	490:496	arg1	stability					498:506	storage stability	490:506	storage stability	490:506	RESULTS This study evaluated the effects of hydrocolloids on the texture properties and storage stability of KC gummy candies.
34894157	4	59	theme	limited	673:679	arg1	seepage					687:693	limited water seepage	673:693	limited water seepage	673:693	The results showed that 4 g kg-1 carboxymethylcellulose (CMC) composited with 20 g kg-1 KC formed a flexible gummy candy with low fragility and limited water seepage during storage.
34894157	3	60	theme	texture	467:473	arg1	properties					475:484	texture properties	467:484	texture properties	467:484	RESULTS This study evaluated the effects of hydrocolloids on the texture properties and storage stability of KC gummy candies.
34894157	4	61	theme	20 g kg-1	607:615	arg1	KC					617:618	20 g kg-1 KC	607:618	20 g kg-1 KC	607:618	The results showed that 4 g kg-1 carboxymethylcellulose (CMC) composited with 20 g kg-1 KC formed a flexible gummy candy with low fragility and limited water seepage during storage.
34894157	8	62	theme	gummy	1511:1515	arg1	candies					1517:1523	vegan gummy candies	1505:1523	vegan gummy candies	1505:1523	It provided preliminary evidence for utilizing hydrocolloids to adjust texture and control water migration in KC gels, and has potential to promote wide development of vegan gummy candies.
34894157	8	63	theme	candies	1517:1523	arg1	development					1490:1500	wide development	1485:1500	wide development of vegan gummy candies	1485:1523	It provided preliminary evidence for utilizing hydrocolloids to adjust texture and control water migration in KC gels, and has potential to promote wide development of vegan gummy candies.
34894157	1	64	theme	plant-based	131:141	arg1	foods					143:147	plant-based foods	131:147	plant-based foods	131:147	BACKGROUND As plant-based foods have become more mainstream in recent years, carrageenan has been used to replace animal-derived gelatin in confectionery products.
34894157	0	65	theme	kappa	64:68	arg1	carrageenan					70:80	kappa carrageenan	64:80	kappa carrageenan	64:80	The improvement of texture properties and storage stability for kappa carrageenan in developing vegan gummy candies.
34894157	1	66	used	used	215:218	arg2	BACKGROUND					117:126	BACKGROUND	117:126	BACKGROUND As plant-based foods have become more mainstream in recent years, carrageenan	117:204	BACKGROUND As plant-based foods have become more mainstream in recent years, carrageenan has been used to replace animal-derived gelatin in confectionery products.
34894157	1	67	from	years	187:191	arg1	mainstream					166:175	mainstream	166:175	mainstream	166:175	BACKGROUND As plant-based foods have become more mainstream in recent years, carrageenan has been used to replace animal-derived gelatin in confectionery products.
34894157	2	68	theme	carrageenan	370:380	arg1	candies					393:399	kappa carrageenan (KC) gummy candies	364:399	kappa carrageenan (KC) gummy candies	364:399	However, texture defects and water seepage during storage limit the development of kappa carrageenan (KC) gummy candies.
34894157	5	69	theme	helices	815:821	arg1	aggregation					797:807	side-by-side intermolecular aggregation	769:807	side-by-side intermolecular aggregation of KC helices	769:821	Further investigation revealed that 4 g kg-1 CMC promoted side-by-side intermolecular aggregation of KC helices through hydrogen bonding, which stabilized a denser network structure compared to the pure KC hydrogel.
34894157	6	70	theme	CMC	941:943	arg1	8-12 g kg-1					958:968	8-12 g kg-1	958:968	8-12 g kg-1	958:968	However, high CMC proportions (8-12 g kg-1 ) led to electrostatic repulsion that dominated in the system, inhibiting the gel-forming process and thus resulting in a weak gel structure with accelerated syneresis.
34894157	6	70	theme	CMC	941:943	arg1	proportions					945:955	high CMC proportions	936:955	high CMC proportions (8-12 g kg-1 )	936:970	However, high CMC proportions (8-12 g kg-1 ) led to electrostatic repulsion that dominated in the system, inhibiting the gel-forming process and thus resulting in a weak gel structure with accelerated syneresis.
34894157	1	71	from	mainstream	166:175	arg1	years					187:191	recent years	180:191	recent years	180:191	BACKGROUND As plant-based foods have become more mainstream in recent years, carrageenan has been used to replace animal-derived gelatin in confectionery products.
34894157	2	72	theme	kappa	364:368	arg1	candies					393:399	kappa carrageenan (KC) gummy candies	364:399	kappa carrageenan (KC) gummy candies	364:399	However, texture defects and water seepage during storage limit the development of kappa carrageenan (KC) gummy candies.
34894157	1	73	theme	animal-derived	231:244	arg1	gelatin					246:252	animal-derived gelatin	231:252	animal-derived gelatin	231:252	BACKGROUND As plant-based foods have become more mainstream in recent years, carrageenan has been used to replace animal-derived gelatin in confectionery products.
33635396	6	0	dep	possible	1110:1117	arg1	reproduce					1119:1127	reproduce	1119:1127	reproduce the MW and G/M ratio	1119:1148	The OTR as a criterion to scale up alginate production allowed to replicate the concentration and the alginate production rate; however, it was not possible reproduce the MW and G/M ratio.
33635396	6	1	theme	G/M	1140:1142	arg1	ratio					1144:1148	the MW and G/M ratio	1129:1148	the MW and G/M ratio	1129:1148	The OTR as a criterion to scale up alginate production allowed to replicate the concentration and the alginate production rate; however, it was not possible reproduce the MW and G/M ratio.
33635396	7	2	from	similar	1253:1259	arg1	scales					1293:1298	both scales	1288:1298	both scales	1288:1298	Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1) the alginate MW was similar (approximately 365 kDa) in both scales.
33635396	2	3	theme	diazotrophic	466:477	arg1	conditions					479:488	diazotrophic conditions	466:488	diazotrophic conditions	466:488	In this study, the MW and G/M ratio were evaluated in cultures of Azotobacter vinelandii to 3 and 30 L scales at different oxygen transfer rates (OTRs) under diazotrophic conditions.
33635396	1	4	dep	guluronic	262:270	arg1	G					273:273	G	273:273	G	273:273	Alginates can be used to elaborate hydrogels, and their properties depend on the molecular weight (MW) and the guluronic (G) and mannuronic (M) composition.
33635396	6	5	theme	MW	1133:1134	arg1	ratio					1144:1148	the MW and G/M ratio	1129:1148	the MW and G/M ratio	1129:1148	The OTR as a criterion to scale up alginate production allowed to replicate the concentration and the alginate production rate; however, it was not possible reproduce the MW and G/M ratio.
33635396	0	6	theme	bioreactor	102:111	arg1	scales					113:118	two bioreactor scales	98:118	two bioreactor scales	98:118	Molecular weight and guluronic/mannuronic ratio of alginate produced by Azotobacter vinelandii at two bioreactor scales under diazotrophic conditions.
33635396	5	7	theme	alginate	845:852	arg1	MW					854:855	A higher alginate MW	836:855	A higher alginate MW (520 ± 43 kDa)	836:870	A higher alginate MW (520 ± 43 kDa) and G/M ratio (0.86 ± 0.01) were obtained in the cultures conducted at 10.4 mmol L-1 h-1.
33635396	5	7	theme	alginate	845:852	arg1	520 ± 43 kDa					858:869	520 ± 43 kDa	858:869	520 ± 43 kDa	858:869	A higher alginate MW (520 ± 43 kDa) and G/M ratio (0.86 ± 0.01) were obtained in the cultures conducted at 10.4 mmol L-1 h-1.
33635396	9	8	theme	alginate	1463:1470	arg1	composition					1472:1482	the alginate composition	1459:1482	the alginate composition	1459:1482	Overall, the results have shown that the alginate composition could be affected by cellular respiration, and from a technological perspective the evidences contribute to the design process based on oxygen consumption to produce alginates defined.
33635396	1	9	used	used	168:171	arg2	Alginates					151:159	Alginates	151:159	Alginates	151:159	Alginates can be used to elaborate hydrogels, and their properties depend on the molecular weight (MW) and the guluronic (G) and mannuronic (M) composition.
33635396	9	10	theme	technological	1538:1550	arg1	perspective					1552:1562	a technological perspective	1536:1562	a technological perspective	1536:1562	Overall, the results have shown that the alginate composition could be affected by cellular respiration, and from a technological perspective the evidences contribute to the design process based on oxygen consumption to produce alginates defined.
33635396	9	11	theme	design	1596:1601	arg1	process					1603:1609	the design process	1592:1609	the design process based on oxygen consumption to produce alginates defined	1592:1666	Overall, the results have shown that the alginate composition could be affected by cellular respiration, and from a technological perspective the evidences contribute to the design process based on oxygen consumption to produce alginates defined.
33635396	4	12	theme	stationary	731:740	arg1	phase					742:746	the stationary phase	727:746	the stationary phase	727:746	In the cultures conducted to an OTR of 10.4 mmol L-1 h-1 (500 rpm), the G/M increased during the cell growth phase and decreased during the stationary phase; whereas, in the cultures at 19.2 mmol L-1 h-1 was constant throughout the cultivation.
33635396	7	13	theme	uptake	1183:1188	arg1	qO2					1196:1198	qO2	1196:1198	qO2	1196:1198	Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1) the alginate MW was similar (approximately 365 kDa) in both scales.
33635396	7	13	theme	uptake	1183:1188	arg1	rate					1190:1193	a similar specific oxygen uptake rate	1157:1193	a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1)	1157:1231	Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1) the alginate MW was similar (approximately 365 kDa) in both scales.
33635396	7	14	theme	specific	1167:1174	arg1	qO2					1196:1198	qO2	1196:1198	qO2	1196:1198	Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1) the alginate MW was similar (approximately 365 kDa) in both scales.
33635396	7	14	theme	specific	1167:1174	arg1	rate					1190:1193	a similar specific oxygen uptake rate	1157:1193	a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1)	1157:1231	Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1) the alginate MW was similar (approximately 365 kDa) in both scales.
33635396	2	15	theme	transfer	438:445	arg1	rates					447:451	different oxygen transfer rates	421:451	different oxygen transfer rates (OTRs)	421:458	In this study, the MW and G/M ratio were evaluated in cultures of Azotobacter vinelandii to 3 and 30 L scales at different oxygen transfer rates (OTRs) under diazotrophic conditions.
33635396	2	15	theme	transfer	438:445	arg1	OTRs					454:457	OTRs	454:457	OTRs	454:457	In this study, the MW and G/M ratio were evaluated in cultures of Azotobacter vinelandii to 3 and 30 L scales at different oxygen transfer rates (OTRs) under diazotrophic conditions.
33635396	7	16	from	scales	1293:1298	arg1	similar					1253:1259	similar	1253:1259	similar	1253:1259	Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1) the alginate MW was similar (approximately 365 kDa) in both scales.
33635396	7	16	from	scales	1293:1298	arg1	365 kDa					1276:1282	365 kDa	1276:1282	365 kDa	1276:1282	Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1) the alginate MW was similar (approximately 365 kDa) in both scales.
33635396	7	16	from	scales	1293:1298	arg1	MW					1246:1247	the alginate MW	1233:1247	the alginate MW	1233:1247	Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1) the alginate MW was similar (approximately 365 kDa) in both scales.
33635396	8	17	theme	bioreactor	1403:1412	arg1	scales					1414:1419	two bioreactor scales	1399:1419	two bioreactor scales	1399:1419	The evidences revealed that the qO2 can be a parameter adequate to produce alginate MW similar in two bioreactor scales.
33635396	2	18	theme	G/M	334:336	arg1	ratio					338:342	G/M ratio	334:342	G/M ratio	334:342	In this study, the MW and G/M ratio were evaluated in cultures of Azotobacter vinelandii to 3 and 30 L scales at different oxygen transfer rates (OTRs) under diazotrophic conditions.
33635396	5	19	theme	10.4 mmol	943:951	arg1	L-1 h-1					953:959	10.4 mmol L-1 h-1	943:959	10.4 mmol L-1 h-1	943:959	A higher alginate MW (520 ± 43 kDa) and G/M ratio (0.86 ± 0.01) were obtained in the cultures conducted at 10.4 mmol L-1 h-1.
33635396	1	20	dep	mannuronic	280:289	arg1	M					292:292	M	292:292	M	292:292	Alginates can be used to elaborate hydrogels, and their properties depend on the molecular weight (MW) and the guluronic (G) and mannuronic (M) composition.
33635396	8	21	from	similar	1388:1394	arg1	scales					1414:1419	two bioreactor scales	1399:1419	two bioreactor scales	1399:1419	The evidences revealed that the qO2 can be a parameter adequate to produce alginate MW similar in two bioreactor scales.
33635396	2	22	theme	oxygen	431:436	arg1	rates					447:451	different oxygen transfer rates	421:451	different oxygen transfer rates (OTRs)	421:458	In this study, the MW and G/M ratio were evaluated in cultures of Azotobacter vinelandii to 3 and 30 L scales at different oxygen transfer rates (OTRs) under diazotrophic conditions.
33635396	2	22	theme	oxygen	431:436	arg1	OTRs					454:457	OTRs	454:457	OTRs	454:457	In this study, the MW and G/M ratio were evaluated in cultures of Azotobacter vinelandii to 3 and 30 L scales at different oxygen transfer rates (OTRs) under diazotrophic conditions.
33635396	7	23	theme	alginate	1237:1244	arg1	similar					1253:1259	similar	1253:1259	similar	1253:1259	Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1) the alginate MW was similar (approximately 365 kDa) in both scales.
33635396	7	23	theme	alginate	1237:1244	arg1	365 kDa					1276:1282	365 kDa	1276:1282	365 kDa	1276:1282	Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1) the alginate MW was similar (approximately 365 kDa) in both scales.
33635396	7	23	theme	alginate	1237:1244	arg1	MW					1246:1247	the alginate MW	1233:1247	the alginate MW	1233:1247	Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1) the alginate MW was similar (approximately 365 kDa) in both scales.
33635396	0	24	theme	Molecular	0:8	arg1	weight					10:15	Molecular weight	0:15	Molecular weight	0:15	Molecular weight and guluronic/mannuronic ratio of alginate produced by Azotobacter vinelandii at two bioreactor scales under diazotrophic conditions.
33635396	1	25	theme	guluronic	262:270	arg1	composition					295:305	the guluronic (G) and mannuronic (M) composition	258:305	the guluronic (G) and mannuronic (M) composition	258:305	Alginates can be used to elaborate hydrogels, and their properties depend on the molecular weight (MW) and the guluronic (G) and mannuronic (M) composition.
33635396	4	26	theme	L-1 h-1	640:646	arg1	OTR					623:625	an OTR	620:625	an OTR of 10.4 mmol L-1 h-1 (500 rpm)	620:656	In the cultures conducted to an OTR of 10.4 mmol L-1 h-1 (500 rpm), the G/M increased during the cell growth phase and decreased during the stationary phase; whereas, in the cultures at 19.2 mmol L-1 h-1 was constant throughout the cultivation.
33635396	7	27	theme	similar	1159:1165	arg1	qO2					1196:1198	qO2	1196:1198	qO2	1196:1198	Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1) the alginate MW was similar (approximately 365 kDa) in both scales.
33635396	7	27	theme	similar	1159:1165	arg1	rate					1190:1193	a similar specific oxygen uptake rate	1157:1193	a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1)	1157:1231	Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1) the alginate MW was similar (approximately 365 kDa) in both scales.
33635396	0	28	theme	guluronic/mannuronic	21:40	arg1	ratio					42:46	guluronic/mannuronic ratio	21:46	guluronic/mannuronic ratio	21:46	Molecular weight and guluronic/mannuronic ratio of alginate produced by Azotobacter vinelandii at two bioreactor scales under diazotrophic conditions.
33635396	5	29	theme	G/M	876:878	arg1	0.86 ± 0.01					887:897	0.86 ± 0.01	887:897	0.86 ± 0.01	887:897	A higher alginate MW (520 ± 43 kDa) and G/M ratio (0.86 ± 0.01) were obtained in the cultures conducted at 10.4 mmol L-1 h-1.
33635396	5	29	theme	G/M	876:878	arg1	ratio					880:884	G/M ratio	876:884	G/M ratio (0.86 ± 0.01)	876:898	A higher alginate MW (520 ± 43 kDa) and G/M ratio (0.86 ± 0.01) were obtained in the cultures conducted at 10.4 mmol L-1 h-1.
33635396	9	30	theme	oxygen	1620:1625	arg1	consumption					1627:1637	oxygen consumption	1620:1637	oxygen consumption	1620:1637	Overall, the results have shown that the alginate composition could be affected by cellular respiration, and from a technological perspective the evidences contribute to the design process based on oxygen consumption to produce alginates defined.
33635396	4	31	from	L-1 h-1	787:793	arg1	cultures					765:772	the cultures	761:772	the cultures at 19.2 mmol L-1 h-1	761:793	In the cultures conducted to an OTR of 10.4 mmol L-1 h-1 (500 rpm), the G/M increased during the cell growth phase and decreased during the stationary phase; whereas, in the cultures at 19.2 mmol L-1 h-1 was constant throughout the cultivation.
33635396	8	32	theme	alginate	1376:1383	arg1	MW					1385:1386	alginate MW	1376:1386	alginate MW similar in two bioreactor scales	1376:1419	The evidences revealed that the qO2 can be a parameter adequate to produce alginate MW similar in two bioreactor scales.
33635396	0	33	theme	diazotrophic	126:137	arg1	conditions					139:148	diazotrophic conditions	126:148	diazotrophic conditions	126:148	Molecular weight and guluronic/mannuronic ratio of alginate produced by Azotobacter vinelandii at two bioreactor scales under diazotrophic conditions.
33635396	5	34	theme	higher	838:843	arg1	MW					854:855	A higher alginate MW	836:855	A higher alginate MW (520 ± 43 kDa)	836:870	A higher alginate MW (520 ± 43 kDa) and G/M ratio (0.86 ± 0.01) were obtained in the cultures conducted at 10.4 mmol L-1 h-1.
33635396	5	34	theme	higher	838:843	arg1	520 ± 43 kDa					858:869	520 ± 43 kDa	858:869	520 ± 43 kDa	858:869	A higher alginate MW (520 ± 43 kDa) and G/M ratio (0.86 ± 0.01) were obtained in the cultures conducted at 10.4 mmol L-1 h-1.
33635396	7	35	dep	rate	1190:1193	arg1	65 mmol g-1 h-1					1216:1230	65 mmol g-1 h-1	1216:1230	65 mmol g-1 h-1	1216:1230	Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1) the alginate MW was similar (approximately 365 kDa) in both scales.
33635396	2	36	theme	L	409:409	arg1	scales					411:416	3 and 30 L scales	400:416	3 and 30 L scales	400:416	In this study, the MW and G/M ratio were evaluated in cultures of Azotobacter vinelandii to 3 and 30 L scales at different oxygen transfer rates (OTRs) under diazotrophic conditions.
33635396	7	37	dep	similar	1253:1259	arg1	similar					1253:1259	similar	1253:1259	similar	1253:1259	Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1) the alginate MW was similar (approximately 365 kDa) in both scales.
33635396	7	37	dep	similar	1253:1259	arg1	365 kDa					1276:1282	365 kDa	1276:1282	365 kDa	1276:1282	Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1) the alginate MW was similar (approximately 365 kDa) in both scales.
33635396	7	37	dep	similar	1253:1259	arg1	MW					1246:1247	the alginate MW	1233:1247	the alginate MW	1233:1247	Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1) the alginate MW was similar (approximately 365 kDa) in both scales.
33635396	8	38	from	scales	1414:1419	arg1	similar					1388:1394	similar	1388:1394	similar	1388:1394	The evidences revealed that the qO2 can be a parameter adequate to produce alginate MW similar in two bioreactor scales.
33635396	8	39	theme	similar	1388:1394	arg1	MW					1385:1386	alginate MW	1376:1386	alginate MW similar in two bioreactor scales	1376:1419	The evidences revealed that the qO2 can be a parameter adequate to produce alginate MW similar in two bioreactor scales.
33635396	3	40	theme	alginate	544:551	arg1	production					553:562	the alginate production	540:562	the alginate production	540:562	An increase in the maximum OTR (OTRmax) improved the alginate production, reaching 3.3 ± 0.2 g L-1.
33635396	2	41	theme	Azotobacter	374:384	arg1	cultures					362:369	cultures	362:369	cultures of Azotobacter vinelandii to 3 and 30 L scales at different oxygen transfer rates (OTRs)	362:458	In this study, the MW and G/M ratio were evaluated in cultures of Azotobacter vinelandii to 3 and 30 L scales at different oxygen transfer rates (OTRs) under diazotrophic conditions.
33635396	1	42	theme	mannuronic	280:289	arg1	composition					295:305	the guluronic (G) and mannuronic (M) composition	258:305	the guluronic (G) and mannuronic (M) composition	258:305	Alginates can be used to elaborate hydrogels, and their properties depend on the molecular weight (MW) and the guluronic (G) and mannuronic (M) composition.
33635396	8	43	theme	adequate	1356:1363	arg1	qO2					1333:1335	the qO2	1329:1335	the qO2	1329:1335	The evidences revealed that the qO2 can be a parameter adequate to produce alginate MW similar in two bioreactor scales.
33635396	8	43	theme	adequate	1356:1363	arg1	parameter					1346:1354	a parameter	1344:1354	a parameter adequate to produce alginate MW similar in two bioreactor scales	1344:1419	The evidences revealed that the qO2 can be a parameter adequate to produce alginate MW similar in two bioreactor scales.
33635396	0	44	theme	alginate	51:58	arg1	weight					10:15	Molecular weight	0:15	Molecular weight	0:15	Molecular weight and guluronic/mannuronic ratio of alginate produced by Azotobacter vinelandii at two bioreactor scales under diazotrophic conditions.
33635396	0	44	theme	alginate	51:58	arg1	ratio					42:46	guluronic/mannuronic ratio	21:46	guluronic/mannuronic ratio	21:46	Molecular weight and guluronic/mannuronic ratio of alginate produced by Azotobacter vinelandii at two bioreactor scales under diazotrophic conditions.
33635396	2	45	from	rates	447:451	arg1	cultures					362:369	cultures	362:369	cultures of Azotobacter vinelandii to 3 and 30 L scales at different oxygen transfer rates (OTRs)	362:458	In this study, the MW and G/M ratio were evaluated in cultures of Azotobacter vinelandii to 3 and 30 L scales at different oxygen transfer rates (OTRs) under diazotrophic conditions.
33635396	3	46	theme	maximum	510:516	arg1	OTR					518:520	the maximum OTR	506:520	the maximum OTR (OTRmax)	506:529	An increase in the maximum OTR (OTRmax) improved the alginate production, reaching 3.3 ± 0.2 g L-1.
33635396	3	46	theme	maximum	510:516	arg1	OTRmax					523:528	OTRmax	523:528	OTRmax	523:528	An increase in the maximum OTR (OTRmax) improved the alginate production, reaching 3.3 ± 0.2 g L-1.
33635396	4	47	from	constant	799:806	arg1	cultures					765:772	the cultures	761:772	the cultures at 19.2 mmol L-1 h-1	761:793	In the cultures conducted to an OTR of 10.4 mmol L-1 h-1 (500 rpm), the G/M increased during the cell growth phase and decreased during the stationary phase; whereas, in the cultures at 19.2 mmol L-1 h-1 was constant throughout the cultivation.
33635396	6	48	theme	production	1073:1082	arg1	rate					1084:1087	the alginate production rate	1060:1087	the alginate production rate	1060:1087	The OTR as a criterion to scale up alginate production allowed to replicate the concentration and the alginate production rate; however, it was not possible reproduce the MW and G/M ratio.
33635396	4	49	theme	10.4 mmol	630:638	arg1	500 rpm					649:655	500 rpm	649:655	500 rpm	649:655	In the cultures conducted to an OTR of 10.4 mmol L-1 h-1 (500 rpm), the G/M increased during the cell growth phase and decreased during the stationary phase; whereas, in the cultures at 19.2 mmol L-1 h-1 was constant throughout the cultivation.
33635396	4	49	theme	10.4 mmol	630:638	arg1	L-1 h-1					640:646	10.4 mmol L-1 h-1	630:646	10.4 mmol L-1 h-1 (500 rpm)	630:656	In the cultures conducted to an OTR of 10.4 mmol L-1 h-1 (500 rpm), the G/M increased during the cell growth phase and decreased during the stationary phase; whereas, in the cultures at 19.2 mmol L-1 h-1 was constant throughout the cultivation.
33635396	6	50	theme	alginate	997:1004	arg1	production					1006:1015	alginate production	997:1015	alginate production	997:1015	The OTR as a criterion to scale up alginate production allowed to replicate the concentration and the alginate production rate; however, it was not possible reproduce the MW and G/M ratio.
33635396	4	51	theme	19.2 mmol	777:785	arg1	L-1 h-1					787:793	19.2 mmol L-1 h-1	777:793	19.2 mmol L-1 h-1	777:793	In the cultures conducted to an OTR of 10.4 mmol L-1 h-1 (500 rpm), the G/M increased during the cell growth phase and decreased during the stationary phase; whereas, in the cultures at 19.2 mmol L-1 h-1 was constant throughout the cultivation.
33635396	3	52	theme	3.3 ± 0.2 g	574:584	arg1	L-1					586:588	3.3 ± 0.2 g L-1	574:588	3.3 ± 0.2 g L-1	574:588	An increase in the maximum OTR (OTRmax) improved the alginate production, reaching 3.3 ± 0.2 g L-1.
33635396	6	53	theme	alginate	1064:1071	arg1	rate					1084:1087	the alginate production rate	1060:1087	the alginate production rate	1060:1087	The OTR as a criterion to scale up alginate production allowed to replicate the concentration and the alginate production rate; however, it was not possible reproduce the MW and G/M ratio.
33635396	3	54	from	increase	494:501	arg1	OTR					518:520	the maximum OTR	506:520	the maximum OTR (OTRmax)	506:529	An increase in the maximum OTR (OTRmax) improved the alginate production, reaching 3.3 ± 0.2 g L-1.
33635396	3	54	from	increase	494:501	arg1	OTRmax					523:528	OTRmax	523:528	OTRmax	523:528	An increase in the maximum OTR (OTRmax) improved the alginate production, reaching 3.3 ± 0.2 g L-1.
33635396	1	55	theme	molecular	232:240	arg1	weight					242:247	the molecular weight	228:247	the molecular weight (MW)	228:252	Alginates can be used to elaborate hydrogels, and their properties depend on the molecular weight (MW) and the guluronic (G) and mannuronic (M) composition.
33635396	1	55	theme	molecular	232:240	arg1	MW					250:251	MW	250:251	MW	250:251	Alginates can be used to elaborate hydrogels, and their properties depend on the molecular weight (MW) and the guluronic (G) and mannuronic (M) composition.
33635396	0	56	theme	Azotobacter	72:82	arg1	vinelandii					84:93	Azotobacter vinelandii	72:93	Azotobacter vinelandii	72:93	Molecular weight and guluronic/mannuronic ratio of alginate produced by Azotobacter vinelandii at two bioreactor scales under diazotrophic conditions.
33635396	4	57	from	cultures	765:772	arg1	constant					799:806	constant	799:806	constant	799:806	In the cultures conducted to an OTR of 10.4 mmol L-1 h-1 (500 rpm), the G/M increased during the cell growth phase and decreased during the stationary phase; whereas, in the cultures at 19.2 mmol L-1 h-1 was constant throughout the cultivation.
33635396	4	58	theme	growth	693:698	arg1	phase					700:704	the cell growth phase	684:704	the cell growth phase	684:704	In the cultures conducted to an OTR of 10.4 mmol L-1 h-1 (500 rpm), the G/M increased during the cell growth phase and decreased during the stationary phase; whereas, in the cultures at 19.2 mmol L-1 h-1 was constant throughout the cultivation.
33635396	7	59	theme	oxygen	1176:1181	arg1	qO2					1196:1198	qO2	1196:1198	qO2	1196:1198	Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1) the alginate MW was similar (approximately 365 kDa) in both scales.
33635396	7	59	theme	oxygen	1176:1181	arg1	rate					1190:1193	a similar specific oxygen uptake rate	1157:1193	a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1)	1157:1231	Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1) the alginate MW was similar (approximately 365 kDa) in both scales.
33635396	2	60	theme	different	421:429	arg1	rates					447:451	different oxygen transfer rates	421:451	different oxygen transfer rates (OTRs)	421:458	In this study, the MW and G/M ratio were evaluated in cultures of Azotobacter vinelandii to 3 and 30 L scales at different oxygen transfer rates (OTRs) under diazotrophic conditions.
33635396	2	60	theme	different	421:429	arg1	OTRs					454:457	OTRs	454:457	OTRs	454:457	In this study, the MW and G/M ratio were evaluated in cultures of Azotobacter vinelandii to 3 and 30 L scales at different oxygen transfer rates (OTRs) under diazotrophic conditions.
33635396	4	61	theme	cell	688:691	arg1	phase					700:704	the cell growth phase	684:704	the cell growth phase	684:704	In the cultures conducted to an OTR of 10.4 mmol L-1 h-1 (500 rpm), the G/M increased during the cell growth phase and decreased during the stationary phase; whereas, in the cultures at 19.2 mmol L-1 h-1 was constant throughout the cultivation.
33635396	2	62	from	cultures	362:369	arg1	rates					447:451	different oxygen transfer rates	421:451	different oxygen transfer rates (OTRs)	421:458	In this study, the MW and G/M ratio were evaluated in cultures of Azotobacter vinelandii to 3 and 30 L scales at different oxygen transfer rates (OTRs) under diazotrophic conditions.
33635396	2	62	from	cultures	362:369	arg1	OTRs					454:457	OTRs	454:457	OTRs	454:457	In this study, the MW and G/M ratio were evaluated in cultures of Azotobacter vinelandii to 3 and 30 L scales at different oxygen transfer rates (OTRs) under diazotrophic conditions.
33635396	9	63	theme	cellular	1505:1512	arg1	respiration					1514:1524	cellular respiration	1505:1524	cellular respiration	1505:1524	Overall, the results have shown that the alginate composition could be affected by cellular respiration, and from a technological perspective the evidences contribute to the design process based on oxygen consumption to produce alginates defined.
33518690	0	0	theme	composite	106:114	arg1	resin					116:120	an experimental composite resin	90:120	an experimental composite resin	90:120	The incorporation of phosphorylated chitosan/amorphous calcium phosphate nanocomplex into an experimental composite resin.
33518690	1	1	theme	chitosan/amorphous	187:204	arg1	Pchi/ACP					237:244	Pchi/ACP	237:244	Pchi/ACP	237:244	This study evaluated the effect of incorporating phosphorylated chitosan/amorphous calcium phosphate nanocomplex (Pchi/ACP) into an experimental light-cure composite resin on mechanical-chemical properties and human dentin remineralization.
33518690	1	1	theme	chitosan/amorphous	187:204	arg1	nanocomplex					224:234	phosphorylated chitosan/amorphous calcium phosphate nanocomplex	172:234	phosphorylated chitosan/amorphous calcium phosphate nanocomplex (Pchi/ACP)	172:245	This study evaluated the effect of incorporating phosphorylated chitosan/amorphous calcium phosphate nanocomplex (Pchi/ACP) into an experimental light-cure composite resin on mechanical-chemical properties and human dentin remineralization.
33518690	1	2	theme	mechanical-chemical	298:316	arg1	properties					318:327	mechanical-chemical properties	298:327	mechanical-chemical properties	298:327	This study evaluated the effect of incorporating phosphorylated chitosan/amorphous calcium phosphate nanocomplex (Pchi/ACP) into an experimental light-cure composite resin on mechanical-chemical properties and human dentin remineralization.
33518690	0	3	theme	experimental	93:104	arg1	resin					116:120	an experimental composite resin	90:120	an experimental composite resin	90:120	The incorporation of phosphorylated chitosan/amorphous calcium phosphate nanocomplex into an experimental composite resin.
33518690	1	4	theme	calcium	206:212	arg1	Pchi/ACP					237:244	Pchi/ACP	237:244	Pchi/ACP	237:244	This study evaluated the effect of incorporating phosphorylated chitosan/amorphous calcium phosphate nanocomplex (Pchi/ACP) into an experimental light-cure composite resin on mechanical-chemical properties and human dentin remineralization.
33518690	1	4	theme	calcium	206:212	arg1	nanocomplex					224:234	phosphorylated chitosan/amorphous calcium phosphate nanocomplex	172:234	phosphorylated chitosan/amorphous calcium phosphate nanocomplex (Pchi/ACP)	172:245	This study evaluated the effect of incorporating phosphorylated chitosan/amorphous calcium phosphate nanocomplex (Pchi/ACP) into an experimental light-cure composite resin on mechanical-chemical properties and human dentin remineralization.
33518690	6	5	theme	due	1081:1083	arg1	resin					1040:1044	the Pchi/ACP-incorporating composite resin	1003:1044	the Pchi/ACP-incorporating composite resin	1003:1044	In conclusion, the Pchi/ACP-incorporating composite resin can be a promising dental material due to its favorable mechanical and remineralization properties.
33518690	6	5	theme	due	1081:1083	arg1	material					1072:1079	a promising dental material	1053:1079	a promising dental material due to its favorable mechanical and remineralization properties	1053:1143	In conclusion, the Pchi/ACP-incorporating composite resin can be a promising dental material due to its favorable mechanical and remineralization properties.
33518690	3	6	theme	Pchi/ACP	622:629	arg1	release					663:669	Pchi/ACP significantly increased calcium release	622:669	Pchi/ACP significantly increased calcium release	622:669	Release concentrations of calcium in saline solution were measured at different time points, showing the incorporation of Pchi/ACP significantly increased calcium release within 14 days, and kept steady thereafter.
33518690	3	7	theme	release	663:669	arg1	incorporation					605:617	the incorporation	601:617	the incorporation of Pchi/ACP significantly increased calcium release	601:669	Release concentrations of calcium in saline solution were measured at different time points, showing the incorporation of Pchi/ACP significantly increased calcium release within 14 days, and kept steady thereafter.
33518690	6	8	theme	Pchi/ACP-incorporating	1007:1028	arg1	resin					1040:1044	the Pchi/ACP-incorporating composite resin	1003:1044	the Pchi/ACP-incorporating composite resin	1003:1044	In conclusion, the Pchi/ACP-incorporating composite resin can be a promising dental material due to its favorable mechanical and remineralization properties.
33518690	6	8	theme	Pchi/ACP-incorporating	1007:1028	arg1	material					1072:1079	a promising dental material	1053:1079	a promising dental material due to its favorable mechanical and remineralization properties	1053:1143	In conclusion, the Pchi/ACP-incorporating composite resin can be a promising dental material due to its favorable mechanical and remineralization properties.
33518690	1	9	theme	human	333:337	arg1	remineralization					346:361	human dentin remineralization	333:361	human dentin remineralization	333:361	This study evaluated the effect of incorporating phosphorylated chitosan/amorphous calcium phosphate nanocomplex (Pchi/ACP) into an experimental light-cure composite resin on mechanical-chemical properties and human dentin remineralization.
33518690	5	10	theme	Various	825:831	arg1	apatite					844:850	apatite	844:850	apatite	844:850	Various amounts of apatite were formed on the dentin slabs which were treated with the resins containing Pchi/ACP, whereas no apatite was formed without Pchi/ACP.
33518690	5	10	theme	Various	825:831	arg1	amounts					833:839	Various amounts	825:839	Various amounts of apatite	825:850	Various amounts of apatite were formed on the dentin slabs which were treated with the resins containing Pchi/ACP, whereas no apatite was formed without Pchi/ACP.
33518690	3	11	theme	different	570:578	arg1	points					585:590	different time points	570:590	different time points	570:590	Release concentrations of calcium in saline solution were measured at different time points, showing the incorporation of Pchi/ACP significantly increased calcium release within 14 days, and kept steady thereafter.
33518690	6	12	from	material	1072:1079	arg1	conclusion					991:1000	conclusion	991:1000	conclusion	991:1000	In conclusion, the Pchi/ACP-incorporating composite resin can be a promising dental material due to its favorable mechanical and remineralization properties.
33518690	1	13	theme	phosphate	214:222	arg1	Pchi/ACP					237:244	Pchi/ACP	237:244	Pchi/ACP	237:244	This study evaluated the effect of incorporating phosphorylated chitosan/amorphous calcium phosphate nanocomplex (Pchi/ACP) into an experimental light-cure composite resin on mechanical-chemical properties and human dentin remineralization.
33518690	1	13	theme	phosphate	214:222	arg1	nanocomplex					224:234	phosphorylated chitosan/amorphous calcium phosphate nanocomplex	172:234	phosphorylated chitosan/amorphous calcium phosphate nanocomplex (Pchi/ACP)	172:245	This study evaluated the effect of incorporating phosphorylated chitosan/amorphous calcium phosphate nanocomplex (Pchi/ACP) into an experimental light-cure composite resin on mechanical-chemical properties and human dentin remineralization.
33518690	6	14	theme	dental	1065:1070	arg1	resin					1040:1044	the Pchi/ACP-incorporating composite resin	1003:1044	the Pchi/ACP-incorporating composite resin	1003:1044	In conclusion, the Pchi/ACP-incorporating composite resin can be a promising dental material due to its favorable mechanical and remineralization properties.
33518690	6	14	theme	dental	1065:1070	arg1	material					1072:1079	a promising dental material	1053:1079	a promising dental material due to its favorable mechanical and remineralization properties	1053:1143	In conclusion, the Pchi/ACP-incorporating composite resin can be a promising dental material due to its favorable mechanical and remineralization properties.
33518690	1	15	from	effect	148:153	arg1	properties					318:327	mechanical-chemical properties	298:327	mechanical-chemical properties	298:327	This study evaluated the effect of incorporating phosphorylated chitosan/amorphous calcium phosphate nanocomplex (Pchi/ACP) into an experimental light-cure composite resin on mechanical-chemical properties and human dentin remineralization.
33518690	1	15	from	effect	148:153	arg1	remineralization					346:361	human dentin remineralization	333:361	human dentin remineralization	333:361	This study evaluated the effect of incorporating phosphorylated chitosan/amorphous calcium phosphate nanocomplex (Pchi/ACP) into an experimental light-cure composite resin on mechanical-chemical properties and human dentin remineralization.
33518690	6	16	theme	mechanical	1102:1111	arg1	properties					1134:1143	its favorable mechanical and remineralization properties	1088:1143	its favorable mechanical and remineralization properties	1088:1143	In conclusion, the Pchi/ACP-incorporating composite resin can be a promising dental material due to its favorable mechanical and remineralization properties.
33518690	3	17	theme	calcium	526:532	arg1	concentrations					508:521	Release concentrations	500:521	Release concentrations of calcium in saline solution	500:551	Release concentrations of calcium in saline solution were measured at different time points, showing the incorporation of Pchi/ACP significantly increased calcium release within 14 days, and kept steady thereafter.
33518690	5	18	contain	containing	919:928	arg2	Pchi/ACP					930:937	Pchi/ACP	930:937	Pchi/ACP	930:937	Various amounts of apatite were formed on the dentin slabs which were treated with the resins containing Pchi/ACP, whereas no apatite was formed without Pchi/ACP.
33518690	5	18	contain	containing	919:928	arg1	resins					912:917	the resins	908:917	the resins containing Pchi/ACP	908:937	Various amounts of apatite were formed on the dentin slabs which were treated with the resins containing Pchi/ACP, whereas no apatite was formed without Pchi/ACP.
33518690	2	19	dep	strength	403:410	arg1	the					388:390	the	388:390	the	388:390	The results showed that the mechanical strength and contact angles of the resins decreased with the increase incorporation of Pchi/ACP.
33518690	3	20	theme	Release	500:506	arg1	concentrations					508:521	Release concentrations	500:521	Release concentrations of calcium in saline solution	500:551	Release concentrations of calcium in saline solution were measured at different time points, showing the incorporation of Pchi/ACP significantly increased calcium release within 14 days, and kept steady thereafter.
33518690	1	21	theme	dentin	339:344	arg1	remineralization					346:361	human dentin remineralization	333:361	human dentin remineralization	333:361	This study evaluated the effect of incorporating phosphorylated chitosan/amorphous calcium phosphate nanocomplex (Pchi/ACP) into an experimental light-cure composite resin on mechanical-chemical properties and human dentin remineralization.
33518690	0	22	theme	phosphorylated	21:34	arg1	nanocomplex					73:83	phosphorylated chitosan/amorphous calcium phosphate nanocomplex	21:83	phosphorylated chitosan/amorphous calcium phosphate nanocomplex	21:83	The incorporation of phosphorylated chitosan/amorphous calcium phosphate nanocomplex into an experimental composite resin.
33518690	3	23	theme	increased	645:653	arg1	release					663:669	Pchi/ACP significantly increased calcium release	622:669	Pchi/ACP significantly increased calcium release	622:669	Release concentrations of calcium in saline solution were measured at different time points, showing the incorporation of Pchi/ACP significantly increased calcium release within 14 days, and kept steady thereafter.
33518690	3	24	theme	calcium	655:661	arg1	release					663:669	Pchi/ACP significantly increased calcium release	622:669	Pchi/ACP significantly increased calcium release	622:669	Release concentrations of calcium in saline solution were measured at different time points, showing the incorporation of Pchi/ACP significantly increased calcium release within 14 days, and kept steady thereafter.
33518690	2	25	theme	Pchi/ACP	490:497	arg1	incorporation					473:485	the increase incorporation	460:485	the increase incorporation of Pchi/ACP	460:497	The results showed that the mechanical strength and contact angles of the resins decreased with the increase incorporation of Pchi/ACP.
33518690	0	26	theme	calcium	55:61	arg1	nanocomplex					73:83	phosphorylated chitosan/amorphous calcium phosphate nanocomplex	21:83	phosphorylated chitosan/amorphous calcium phosphate nanocomplex	21:83	The incorporation of phosphorylated chitosan/amorphous calcium phosphate nanocomplex into an experimental composite resin.
33518690	4	27	theme	demineralized	728:740	arg1	slabs					749:753	the demineralized dentin slabs	724:753	the demineralized dentin slabs treated with our resins for four weeks	724:792	Finally, the demineralized dentin slabs treated with our resins for four weeks were characterized by SEM-EDS.
33518690	0	28	theme	chitosan/amorphous	36:53	arg1	nanocomplex					73:83	phosphorylated chitosan/amorphous calcium phosphate nanocomplex	21:83	phosphorylated chitosan/amorphous calcium phosphate nanocomplex	21:83	The incorporation of phosphorylated chitosan/amorphous calcium phosphate nanocomplex into an experimental composite resin.
33518690	1	29	theme	experimental	255:266	arg1	resin					289:293	an experimental light-cure composite resin	252:293	an experimental light-cure composite resin	252:293	This study evaluated the effect of incorporating phosphorylated chitosan/amorphous calcium phosphate nanocomplex (Pchi/ACP) into an experimental light-cure composite resin on mechanical-chemical properties and human dentin remineralization.
33518690	0	30	theme	nanocomplex	73:83	arg1	incorporation					4:16	The incorporation	0:16	The incorporation of phosphorylated chitosan/amorphous calcium phosphate nanocomplex into an experimental composite resin.	0:121	The incorporation of phosphorylated chitosan/amorphous calcium phosphate nanocomplex into an experimental composite resin.
33518690	6	31	theme	composite	1030:1038	arg1	resin					1040:1044	the Pchi/ACP-incorporating composite resin	1003:1044	the Pchi/ACP-incorporating composite resin	1003:1044	In conclusion, the Pchi/ACP-incorporating composite resin can be a promising dental material due to its favorable mechanical and remineralization properties.
33518690	6	31	theme	composite	1030:1038	arg1	material					1072:1079	a promising dental material	1053:1079	a promising dental material due to its favorable mechanical and remineralization properties	1053:1143	In conclusion, the Pchi/ACP-incorporating composite resin can be a promising dental material due to its favorable mechanical and remineralization properties.
33518690	6	32	theme	remineralization	1117:1132	arg1	properties					1134:1143	its favorable mechanical and remineralization properties	1088:1143	its favorable mechanical and remineralization properties	1088:1143	In conclusion, the Pchi/ACP-incorporating composite resin can be a promising dental material due to its favorable mechanical and remineralization properties.
33518690	5	33	theme	apatite	844:850	arg1	apatite					844:850	apatite	844:850	apatite	844:850	Various amounts of apatite were formed on the dentin slabs which were treated with the resins containing Pchi/ACP, whereas no apatite was formed without Pchi/ACP.
33518690	5	33	theme	apatite	844:850	arg1	amounts					833:839	Various amounts	825:839	Various amounts of apatite	825:850	Various amounts of apatite were formed on the dentin slabs which were treated with the resins containing Pchi/ACP, whereas no apatite was formed without Pchi/ACP.
33518690	2	34	theme	contact	416:422	arg1	angles					424:429	contact angles	416:429	contact angles	416:429	The results showed that the mechanical strength and contact angles of the resins decreased with the increase incorporation of Pchi/ACP.
33518690	0	35	theme	phosphate	63:71	arg1	nanocomplex					73:83	phosphorylated chitosan/amorphous calcium phosphate nanocomplex	21:83	phosphorylated chitosan/amorphous calcium phosphate nanocomplex	21:83	The incorporation of phosphorylated chitosan/amorphous calcium phosphate nanocomplex into an experimental composite resin.
33518690	2	36	theme	increase	464:471	arg1	incorporation					473:485	the increase incorporation	460:485	the increase incorporation of Pchi/ACP	460:497	The results showed that the mechanical strength and contact angles of the resins decreased with the increase incorporation of Pchi/ACP.
33518690	3	37	theme	time	580:583	arg1	points					585:590	different time points	570:590	different time points	570:590	Release concentrations of calcium in saline solution were measured at different time points, showing the incorporation of Pchi/ACP significantly increased calcium release within 14 days, and kept steady thereafter.
33518690	1	38	theme	light-cure	268:277	arg1	resin					289:293	an experimental light-cure composite resin	252:293	an experimental light-cure composite resin	252:293	This study evaluated the effect of incorporating phosphorylated chitosan/amorphous calcium phosphate nanocomplex (Pchi/ACP) into an experimental light-cure composite resin on mechanical-chemical properties and human dentin remineralization.
33518690	2	39	theme	mechanical	392:401	arg1	strength					403:410	mechanical strength	392:410	mechanical strength	392:410	The results showed that the mechanical strength and contact angles of the resins decreased with the increase incorporation of Pchi/ACP.
33518690	1	40	theme	composite	279:287	arg1	resin					289:293	an experimental light-cure composite resin	252:293	an experimental light-cure composite resin	252:293	This study evaluated the effect of incorporating phosphorylated chitosan/amorphous calcium phosphate nanocomplex (Pchi/ACP) into an experimental light-cure composite resin on mechanical-chemical properties and human dentin remineralization.
33518690	3	41	theme	saline	537:542	arg1	solution					544:551	saline solution	537:551	saline solution	537:551	Release concentrations of calcium in saline solution were measured at different time points, showing the incorporation of Pchi/ACP significantly increased calcium release within 14 days, and kept steady thereafter.
33518690	3	42	from	concentrations	508:521	arg1	solution					544:551	saline solution	537:551	saline solution	537:551	Release concentrations of calcium in saline solution were measured at different time points, showing the incorporation of Pchi/ACP significantly increased calcium release within 14 days, and kept steady thereafter.
33518690	2	43	theme	resins	438:443	arg1	strength					403:410	mechanical strength	392:410	mechanical strength	392:410	The results showed that the mechanical strength and contact angles of the resins decreased with the increase incorporation of Pchi/ACP.
33518690	2	43	theme	resins	438:443	arg1	angles					424:429	contact angles	416:429	contact angles	416:429	The results showed that the mechanical strength and contact angles of the resins decreased with the increase incorporation of Pchi/ACP.
33518690	4	44	theme	dentin	742:747	arg1	slabs					749:753	the demineralized dentin slabs	724:753	the demineralized dentin slabs treated with our resins for four weeks	724:792	Finally, the demineralized dentin slabs treated with our resins for four weeks were characterized by SEM-EDS.
33518690	6	45	theme	favorable	1092:1100	arg1	properties					1134:1143	its favorable mechanical and remineralization properties	1088:1143	its favorable mechanical and remineralization properties	1088:1143	In conclusion, the Pchi/ACP-incorporating composite resin can be a promising dental material due to its favorable mechanical and remineralization properties.
33518690	5	46	theme	dentin	871:876	arg1	slabs					878:882	the dentin slabs	867:882	the dentin slabs which were treated with the resins containing Pchi/ACP	867:937	Various amounts of apatite were formed on the dentin slabs which were treated with the resins containing Pchi/ACP, whereas no apatite was formed without Pchi/ACP.
33518690	6	47	theme	promising	1055:1063	arg1	resin					1040:1044	the Pchi/ACP-incorporating composite resin	1003:1044	the Pchi/ACP-incorporating composite resin	1003:1044	In conclusion, the Pchi/ACP-incorporating composite resin can be a promising dental material due to its favorable mechanical and remineralization properties.
33518690	6	47	theme	promising	1055:1063	arg1	material					1072:1079	a promising dental material	1053:1079	a promising dental material due to its favorable mechanical and remineralization properties	1053:1143	In conclusion, the Pchi/ACP-incorporating composite resin can be a promising dental material due to its favorable mechanical and remineralization properties.
33518690	1	48	theme	phosphorylated	172:185	arg1	Pchi/ACP					237:244	Pchi/ACP	237:244	Pchi/ACP	237:244	This study evaluated the effect of incorporating phosphorylated chitosan/amorphous calcium phosphate nanocomplex (Pchi/ACP) into an experimental light-cure composite resin on mechanical-chemical properties and human dentin remineralization.
33518690	1	48	theme	phosphorylated	172:185	arg1	nanocomplex					224:234	phosphorylated chitosan/amorphous calcium phosphate nanocomplex	172:234	phosphorylated chitosan/amorphous calcium phosphate nanocomplex (Pchi/ACP)	172:245	This study evaluated the effect of incorporating phosphorylated chitosan/amorphous calcium phosphate nanocomplex (Pchi/ACP) into an experimental light-cure composite resin on mechanical-chemical properties and human dentin remineralization.
32416684	4	0	theme	uncoated	658:665	arg1	microparticles					688:701	The spray-dried uncoated and chitosan- coated microparticles	642:701	The spray-dried uncoated and chitosan- coated microparticles	642:701	The spray-dried uncoated and chitosan- coated microparticles were characterized for various parameters (Particle size, Morphology, Drug entrapment efficiency).
32416684	6	1	theme	Ex	903:904	arg1	studies					911:917	Ex vivo studies	903:917	Ex vivo studies	903:917	Ex vivo studies were carried out to evaluate the cytotoxic potential of the developed formulation by the MTT assay.
32416684	3	2	theme	ionotropic	569:578	arg1	technique					589:597	ionotropic gelation technique	569:597	ionotropic gelation technique	569:597	METHODS Cisplatin loaded chitosan coated spray-dried microparticles were prepared by ionotropic gelation technique and optimized by Central Composite Design.
32416684	6	3	theme	developed	979:987	arg1	formulation					989:999	the developed formulation	975:999	the developed formulation	975:999	Ex vivo studies were carried out to evaluate the cytotoxic potential of the developed formulation by the MTT assay.
32416684	4	4	theme	chitosan-	671:679	arg1	microparticles					688:701	The spray-dried uncoated and chitosan- coated microparticles	642:701	The spray-dried uncoated and chitosan- coated microparticles	642:701	The spray-dried uncoated and chitosan- coated microparticles were characterized for various parameters (Particle size, Morphology, Drug entrapment efficiency).
32416684	3	5	theme	Central	616:622	arg1	Design					634:639	Central Composite Design	616:639	Central Composite Design	616:639	METHODS Cisplatin loaded chitosan coated spray-dried microparticles were prepared by ionotropic gelation technique and optimized by Central Composite Design.
32416684	12	6	theme	in	1526:1527	arg1	regression					1540:1549	in vivo tumor regression	1526:1549	in vivo tumor regression	1526:1549	Finally, in vivo tumor regression and histopathological studies confirmed the significant decrease in tumor volume at different time intervals.
32416684	12	7	theme	tumor	1534:1538	arg1	regression					1540:1549	in vivo tumor regression	1526:1549	in vivo tumor regression	1526:1549	Finally, in vivo tumor regression and histopathological studies confirmed the significant decrease in tumor volume at different time intervals.
32416684	6	8	theme	formulation	989:999	arg1	potential					962:970	the cytotoxic potential	948:970	the cytotoxic potential of the developed formulation	948:999	Ex vivo studies were carried out to evaluate the cytotoxic potential of the developed formulation by the MTT assay.
32416684	1	9	theme	cancer	211:216	arg1	treatment					189:197	The existing parenteral treatment	165:197	The existing parenteral treatment of cervical cancer	165:216	BACKGROUND The existing parenteral treatment of cervical cancer has high toxicity and poor distribution of drugs at the targeted site.
32416684	4	10	theme	various	726:732	arg1	parameters					734:743	various parameters	726:743	various parameters (Particle size, Morphology, Drug entrapment efficiency)	726:799	The spray-dried uncoated and chitosan- coated microparticles were characterized for various parameters (Particle size, Morphology, Drug entrapment efficiency).
32416684	2	11	theme	localized	310:318	arg1	cisplatin					333:341	localized mucoadhesive cisplatin	310:341	localized mucoadhesive cisplatin	310:341	PURPOSE To formulate localized mucoadhesive cisplatin loaded microparticles based formulation to treat cervical cancer so that enhanced therapeutics benefits with low toxicity could be achieved.
32416684	1	12	theme	high	222:225	arg1	toxicity					227:234	high toxicity	222:234	high toxicity	222:234	BACKGROUND The existing parenteral treatment of cervical cancer has high toxicity and poor distribution of drugs at the targeted site.
32416684	0	13	theme	Cancer	146:151	arg1	Treatment					124:132	the Treatment	120:132	the Treatment of Cervical Cancer	120:151	DoE Based Optimization and Development of Spray-Dried Chitosan-Coated Alginate Microparticles Loaded with Cisplatin for the Treatment of Cervical Cancer.
32416684	8	14	dep	in	1142:1143	arg1	vitro					1145:1149	vitro	1145:1149	vitro	1145:1149	RESULTS All in vitro characterization parameters were found to be optimum.
32416684	7	15	theme	Albino	1117:1122	arg1	Mice					1124:1127	Swiss Albino Mice	1111:1127	Swiss Albino Mice	1111:1127	A drug permeability study was performed by Franz diffusion cell using the vaginal tissue of Swiss Albino Mice.
32416684	6	16	theme	MTT	1008:1010	arg1	assay					1012:1016	the MTT assay	1004:1016	the MTT assay	1004:1016	Ex vivo studies were carried out to evaluate the cytotoxic potential of the developed formulation by the MTT assay.
32416684	7	17	theme	Franz	1062:1066	arg1	cell					1078:1081	Franz diffusion cell	1062:1081	Franz diffusion cell	1062:1081	A drug permeability study was performed by Franz diffusion cell using the vaginal tissue of Swiss Albino Mice.
32416684	5	18	dep	In	802:803	arg1	vitro					805:809	vitro	805:809	vitro	805:809	In vitro drug release study was carried out in simulated vaginal fluids by dialysis membrane method.
32416684	4	19	theme	Drug	773:776	arg1	size					755:758	Particle size	746:758	Particle size	746:758	The spray-dried uncoated and chitosan- coated microparticles were characterized for various parameters (Particle size, Morphology, Drug entrapment efficiency).
32416684	4	19	theme	Drug	773:776	arg1	efficiency					789:798	Drug entrapment efficiency	773:798	Drug entrapment efficiency	773:798	The spray-dried uncoated and chitosan- coated microparticles were characterized for various parameters (Particle size, Morphology, Drug entrapment efficiency).
32416684	0	20	theme	Alginate	70:77	arg1	Microparticles					79:92	Spray-Dried Chitosan-Coated Alginate Microparticles	42:92	Spray-Dried Chitosan-Coated Alginate Microparticles Loaded with Cisplatin for the Treatment of Cervical Cancer	42:151	DoE Based Optimization and Development of Spray-Dried Chitosan-Coated Alginate Microparticles Loaded with Cisplatin for the Treatment of Cervical Cancer.
32416684	13	21	with	route	1836:1840	arg1	effectiveness					1884:1896	its enhanced effectiveness	1871:1896	its enhanced effectiveness	1871:1896	CONCLUSION Thus, it can be concluded that mucoadhesive spray-dried microparticles could provide a favorable approach for localized delivery of the anticancer drug via vaginal route against cervical cancer with its enhanced effectiveness.
32416684	5	22	theme	simulated	849:857	arg1	fluids					867:872	simulated vaginal fluids	849:872	simulated vaginal fluids	849:872	In vitro drug release study was carried out in simulated vaginal fluids by dialysis membrane method.
32416684	1	23	theme	parenteral	178:187	arg1	treatment					189:197	The existing parenteral treatment	165:197	The existing parenteral treatment of cervical cancer	165:216	BACKGROUND The existing parenteral treatment of cervical cancer has high toxicity and poor distribution of drugs at the targeted site.
32416684	12	24	theme	time	1645:1648	arg1	intervals					1650:1658	different time intervals	1635:1658	different time intervals	1635:1658	Finally, in vivo tumor regression and histopathological studies confirmed the significant decrease in tumor volume at different time intervals.
32416684	13	25	theme	cervical	1850:1857	arg1	cancer					1859:1864	cervical cancer	1850:1864	cervical cancer	1850:1864	CONCLUSION Thus, it can be concluded that mucoadhesive spray-dried microparticles could provide a favorable approach for localized delivery of the anticancer drug via vaginal route against cervical cancer with its enhanced effectiveness.
32416684	5	26	theme	release	816:822	arg1	study					824:828	In vitro drug release study	802:828	In vitro drug release study	802:828	In vitro drug release study was carried out in simulated vaginal fluids by dialysis membrane method.
32416684	7	27	theme	drug	1021:1024	arg1	study					1039:1043	A drug permeability study	1019:1043	A drug permeability study	1019:1043	A drug permeability study was performed by Franz diffusion cell using the vaginal tissue of Swiss Albino Mice.
32416684	13	28	theme	anticancer	1808:1817	arg1	drug					1819:1822	the anticancer drug	1804:1822	the anticancer drug	1804:1822	CONCLUSION Thus, it can be concluded that mucoadhesive spray-dried microparticles could provide a favorable approach for localized delivery of the anticancer drug via vaginal route against cervical cancer with its enhanced effectiveness.
32416684	10	29	theme	uncoated	1366:1373	arg1	microparticles					1375:1388	uncoated microparticles	1366:1388	uncoated microparticles	1366:1388	The chitosan-coated microparticles were found to be more cytotoxic than uncoated microparticles and plain cisplatin solution.
32416684	2	30	dep	based	365:369	arg1	formulate					300:308	formulate	300:308	To formulate localized mucoadhesive cisplatin	297:341	PURPOSE To formulate localized mucoadhesive cisplatin loaded microparticles based formulation to treat cervical cancer so that enhanced therapeutics benefits with low toxicity could be achieved.
32416684	2	31	theme	enhanced	416:423	arg1	benefits					438:445	enhanced therapeutics benefits	416:445	enhanced therapeutics benefits with low toxicity	416:463	PURPOSE To formulate localized mucoadhesive cisplatin loaded microparticles based formulation to treat cervical cancer so that enhanced therapeutics benefits with low toxicity could be achieved.
32416684	13	32	theme	localized	1782:1790	arg1	delivery					1792:1799	localized delivery	1782:1799	localized delivery of the anticancer drug via vaginal route against cervical cancer with its enhanced effectiveness	1782:1896	CONCLUSION Thus, it can be concluded that mucoadhesive spray-dried microparticles could provide a favorable approach for localized delivery of the anticancer drug via vaginal route against cervical cancer with its enhanced effectiveness.
32416684	1	33	dep	BACKGROUND	154:163	arg1	has					218:220	has	218:220	has high toxicity and poor distribution of drugs at the targeted site	218:286	BACKGROUND The existing parenteral treatment of cervical cancer has high toxicity and poor distribution of drugs at the targeted site.
32416684	10	34	theme	cisplatin	1400:1408	arg1	solution					1410:1417	plain cisplatin solution	1394:1417	plain cisplatin solution	1394:1417	The chitosan-coated microparticles were found to be more cytotoxic than uncoated microparticles and plain cisplatin solution.
32416684	3	35	theme	chitosan	509:516	arg1	microparticles					537:550	Cisplatin loaded chitosan coated spray-dried microparticles	492:550	METHODS Cisplatin loaded chitosan coated spray-dried microparticles	484:550	METHODS Cisplatin loaded chitosan coated spray-dried microparticles were prepared by ionotropic gelation technique and optimized by Central Composite Design.
32416684	8	36	theme	in	1142:1143	arg1	parameters					1168:1177	All in vitro characterization parameters	1138:1177	All in vitro characterization parameters	1138:1177	RESULTS All in vitro characterization parameters were found to be optimum.
32416684	13	37	dep	CONCLUSION	1661:1670	arg1	concluded					1688:1696	concluded	1688:1696	can be concluded that mucoadhesive spray-dried microparticles could provide a favorable approach for localized delivery of the anticancer drug via vaginal route against cervical cancer with its enhanced effectiveness	1681:1896	CONCLUSION Thus, it can be concluded that mucoadhesive spray-dried microparticles could provide a favorable approach for localized delivery of the anticancer drug via vaginal route against cervical cancer with its enhanced effectiveness.
32416684	2	38	theme	low	452:454	arg1	toxicity					456:463	low toxicity	452:463	low toxicity	452:463	PURPOSE To formulate localized mucoadhesive cisplatin loaded microparticles based formulation to treat cervical cancer so that enhanced therapeutics benefits with low toxicity could be achieved.
32416684	3	39	theme	Cisplatin	492:500	arg1	microparticles					537:550	Cisplatin loaded chitosan coated spray-dried microparticles	492:550	METHODS Cisplatin loaded chitosan coated spray-dried microparticles	484:550	METHODS Cisplatin loaded chitosan coated spray-dried microparticles were prepared by ionotropic gelation technique and optimized by Central Composite Design.
32416684	8	40	theme	characterization	1151:1166	arg1	parameters					1168:1177	All in vitro characterization parameters	1138:1177	All in vitro characterization parameters	1138:1177	RESULTS All in vitro characterization parameters were found to be optimum.
32416684	12	41	theme	different	1635:1643	arg1	intervals					1650:1658	different time intervals	1635:1658	different time intervals	1635:1658	Finally, in vivo tumor regression and histopathological studies confirmed the significant decrease in tumor volume at different time intervals.
32416684	4	42	dep	parameters	734:743	arg1	size					755:758	Particle size	746:758	Particle size	746:758	The spray-dried uncoated and chitosan- coated microparticles were characterized for various parameters (Particle size, Morphology, Drug entrapment efficiency).
32416684	4	42	dep	parameters	734:743	arg1	efficiency					789:798	Drug entrapment efficiency	773:798	Drug entrapment efficiency	773:798	The spray-dried uncoated and chitosan- coated microparticles were characterized for various parameters (Particle size, Morphology, Drug entrapment efficiency).
32416684	4	42	dep	parameters	734:743	arg1	Morphology					761:770	Morphology	761:770	Morphology	761:770	The spray-dried uncoated and chitosan- coated microparticles were characterized for various parameters (Particle size, Morphology, Drug entrapment efficiency).
32416684	5	43	theme	dialysis	877:884	arg1	method					895:900	dialysis membrane method	877:900	dialysis membrane method	877:900	In vitro drug release study was carried out in simulated vaginal fluids by dialysis membrane method.
32416684	12	44	theme	significant	1595:1605	arg1	decrease					1607:1614	the significant decrease	1591:1614	the significant decrease in tumor volume	1591:1630	Finally, in vivo tumor regression and histopathological studies confirmed the significant decrease in tumor volume at different time intervals.
32416684	3	45	dep	METHODS	484:490	arg1	microparticles					537:550	Cisplatin loaded chitosan coated spray-dried microparticles	492:550	METHODS Cisplatin loaded chitosan coated spray-dried microparticles	484:550	METHODS Cisplatin loaded chitosan coated spray-dried microparticles were prepared by ionotropic gelation technique and optimized by Central Composite Design.
32416684	9	46	theme	in	1209:1210	arg1	studies					1226:1232	The in vitro release studies	1205:1232	The in vitro release studies	1205:1232	The in vitro release studies indicated a controlled release following the Higuchi model.
32416684	3	47	theme	spray-dried	525:535	arg1	microparticles					537:550	Cisplatin loaded chitosan coated spray-dried microparticles	492:550	METHODS Cisplatin loaded chitosan coated spray-dried microparticles	484:550	METHODS Cisplatin loaded chitosan coated spray-dried microparticles were prepared by ionotropic gelation technique and optimized by Central Composite Design.
32416684	1	48	theme	poor	240:243	arg1	distribution					245:256	poor distribution	240:256	poor distribution	240:256	BACKGROUND The existing parenteral treatment of cervical cancer has high toxicity and poor distribution of drugs at the targeted site.
32416684	4	49	theme	coated	681:686	arg1	microparticles					688:701	The spray-dried uncoated and chitosan- coated microparticles	642:701	The spray-dried uncoated and chitosan- coated microparticles	642:701	The spray-dried uncoated and chitosan- coated microparticles were characterized for various parameters (Particle size, Morphology, Drug entrapment efficiency).
32416684	1	50	contain	has	218:220	arg1	treatment					189:197	The existing parenteral treatment	165:197	The existing parenteral treatment of cervical cancer	165:216	BACKGROUND The existing parenteral treatment of cervical cancer has high toxicity and poor distribution of drugs at the targeted site.
32416684	1	50	contain	has	218:220	arg2	toxicity					227:234	high toxicity	222:234	high toxicity	222:234	BACKGROUND The existing parenteral treatment of cervical cancer has high toxicity and poor distribution of drugs at the targeted site.
32416684	1	50	contain	has	218:220	arg2	distribution					245:256	poor distribution	240:256	poor distribution	240:256	BACKGROUND The existing parenteral treatment of cervical cancer has high toxicity and poor distribution of drugs at the targeted site.
32416684	9	51	theme	release	1218:1224	arg1	studies					1226:1232	The in vitro release studies	1205:1232	The in vitro release studies	1205:1232	The in vitro release studies indicated a controlled release following the Higuchi model.
32416684	3	52	theme	gelation	580:587	arg1	technique					589:597	ionotropic gelation technique	569:597	ionotropic gelation technique	569:597	METHODS Cisplatin loaded chitosan coated spray-dried microparticles were prepared by ionotropic gelation technique and optimized by Central Composite Design.
32416684	12	53	theme	histopathological	1555:1571	arg1	studies					1573:1579	histopathological studies	1555:1579	histopathological studies	1555:1579	Finally, in vivo tumor regression and histopathological studies confirmed the significant decrease in tumor volume at different time intervals.
32416684	6	54	theme	cytotoxic	952:960	arg1	potential					962:970	the cytotoxic potential	948:970	the cytotoxic potential of the developed formulation	948:999	Ex vivo studies were carried out to evaluate the cytotoxic potential of the developed formulation by the MTT assay.
32416684	2	55	theme	cervical	392:399	arg1	cancer					401:406	cervical cancer	392:406	cervical cancer so that enhanced therapeutics benefits with low toxicity could be achieved	392:481	PURPOSE To formulate localized mucoadhesive cisplatin loaded microparticles based formulation to treat cervical cancer so that enhanced therapeutics benefits with low toxicity could be achieved.
32416684	2	56	with	benefits	438:445	arg1	toxicity					456:463	low toxicity	452:463	low toxicity	452:463	PURPOSE To formulate localized mucoadhesive cisplatin loaded microparticles based formulation to treat cervical cancer so that enhanced therapeutics benefits with low toxicity could be achieved.
32416684	4	57	theme	spray-dried	646:656	arg1	microparticles					688:701	The spray-dried uncoated and chitosan- coated microparticles	642:701	The spray-dried uncoated and chitosan- coated microparticles	642:701	The spray-dried uncoated and chitosan- coated microparticles were characterized for various parameters (Particle size, Morphology, Drug entrapment efficiency).
32416684	9	58	theme	controlled	1246:1255	arg1	release					1257:1263	a controlled release	1244:1263	a controlled release following the Higuchi model	1244:1291	The in vitro release studies indicated a controlled release following the Higuchi model.
32416684	13	59	theme	spray-dried	1716:1726	arg1	microparticles					1728:1741	mucoadhesive spray-dried microparticles	1703:1741	mucoadhesive spray-dried microparticles	1703:1741	CONCLUSION Thus, it can be concluded that mucoadhesive spray-dried microparticles could provide a favorable approach for localized delivery of the anticancer drug via vaginal route against cervical cancer with its enhanced effectiveness.
32416684	1	60	theme	targeted	274:281	arg1	site					283:286	the targeted site	270:286	the targeted site	270:286	BACKGROUND The existing parenteral treatment of cervical cancer has high toxicity and poor distribution of drugs at the targeted site.
32416684	0	61	theme	Spray-Dried	42:52	arg1	Microparticles					79:92	Spray-Dried Chitosan-Coated Alginate Microparticles	42:92	Spray-Dried Chitosan-Coated Alginate Microparticles Loaded with Cisplatin for the Treatment of Cervical Cancer	42:151	DoE Based Optimization and Development of Spray-Dried Chitosan-Coated Alginate Microparticles Loaded with Cisplatin for the Treatment of Cervical Cancer.
32416684	4	62	theme	Particle	746:753	arg1	size					755:758	Particle size	746:758	Particle size	746:758	The spray-dried uncoated and chitosan- coated microparticles were characterized for various parameters (Particle size, Morphology, Drug entrapment efficiency).
32416684	4	62	theme	Particle	746:753	arg1	efficiency					789:798	Drug entrapment efficiency	773:798	Drug entrapment efficiency	773:798	The spray-dried uncoated and chitosan- coated microparticles were characterized for various parameters (Particle size, Morphology, Drug entrapment efficiency).
32416684	4	62	theme	Particle	746:753	arg1	Morphology					761:770	Morphology	761:770	Morphology	761:770	The spray-dried uncoated and chitosan- coated microparticles were characterized for various parameters (Particle size, Morphology, Drug entrapment efficiency).
32416684	11	63	theme	chitosan-coated	1424:1438	arg1	microparticles					1440:1453	The chitosan-coated microparticles	1420:1453	The chitosan-coated microparticles	1420:1453	The chitosan-coated microparticles were found to be more permeable than uncoated microparticles.
32416684	11	63	theme	chitosan-coated	1424:1438	arg1	permeable					1477:1485	permeable	1477:1485	permeable	1477:1485	The chitosan-coated microparticles were found to be more permeable than uncoated microparticles.
32416684	9	64	theme	Higuchi	1279:1285	arg1	model					1287:1291	the Higuchi model	1275:1291	the Higuchi model	1275:1291	The in vitro release studies indicated a controlled release following the Higuchi model.
32416684	2	65	theme	loaded	343:348	arg1	microparticles					350:363	loaded microparticles	343:363	loaded microparticles	343:363	PURPOSE To formulate localized mucoadhesive cisplatin loaded microparticles based formulation to treat cervical cancer so that enhanced therapeutics benefits with low toxicity could be achieved.
32416684	1	66	theme	cervical	202:209	arg1	cancer					211:216	cervical cancer	202:216	cervical cancer	202:216	BACKGROUND The existing parenteral treatment of cervical cancer has high toxicity and poor distribution of drugs at the targeted site.
32416684	0	67	theme	Cervical	137:144	arg1	Cancer					146:151	Cervical Cancer	137:151	Cervical Cancer	137:151	DoE Based Optimization and Development of Spray-Dried Chitosan-Coated Alginate Microparticles Loaded with Cisplatin for the Treatment of Cervical Cancer.
32416684	3	68	theme	Composite	624:632	arg1	Design					634:639	Central Composite Design	616:639	Central Composite Design	616:639	METHODS Cisplatin loaded chitosan coated spray-dried microparticles were prepared by ionotropic gelation technique and optimized by Central Composite Design.
32416684	6	69	dep	Ex	903:904	arg1	vivo					906:909	vivo	906:909	vivo	906:909	Ex vivo studies were carried out to evaluate the cytotoxic potential of the developed formulation by the MTT assay.
32416684	12	70	dep	in	1526:1527	arg1	vivo					1529:1532	vivo	1529:1532	vivo	1529:1532	Finally, in vivo tumor regression and histopathological studies confirmed the significant decrease in tumor volume at different time intervals.
32416684	7	71	theme	Mice	1124:1127	arg1	tissue					1101:1106	the vaginal tissue	1089:1106	the vaginal tissue of Swiss Albino Mice	1089:1127	A drug permeability study was performed by Franz diffusion cell using the vaginal tissue of Swiss Albino Mice.
32416684	12	72	from	decrease	1607:1614	arg1	volume					1625:1630	tumor volume	1619:1630	tumor volume	1619:1630	Finally, in vivo tumor regression and histopathological studies confirmed the significant decrease in tumor volume at different time intervals.
32416684	13	73	theme	enhanced	1875:1882	arg1	effectiveness					1884:1896	its enhanced effectiveness	1871:1896	its enhanced effectiveness	1871:1896	CONCLUSION Thus, it can be concluded that mucoadhesive spray-dried microparticles could provide a favorable approach for localized delivery of the anticancer drug via vaginal route against cervical cancer with its enhanced effectiveness.
32416684	7	74	theme	Swiss	1111:1115	arg1	Mice					1124:1127	Swiss Albino Mice	1111:1127	Swiss Albino Mice	1111:1127	A drug permeability study was performed by Franz diffusion cell using the vaginal tissue of Swiss Albino Mice.
32416684	7	75	theme	vaginal	1093:1099	arg1	tissue					1101:1106	the vaginal tissue	1089:1106	the vaginal tissue of Swiss Albino Mice	1089:1127	A drug permeability study was performed by Franz diffusion cell using the vaginal tissue of Swiss Albino Mice.
32416684	2	76	theme	mucoadhesive	320:331	arg1	cisplatin					333:341	localized mucoadhesive cisplatin	310:341	localized mucoadhesive cisplatin	310:341	PURPOSE To formulate localized mucoadhesive cisplatin loaded microparticles based formulation to treat cervical cancer so that enhanced therapeutics benefits with low toxicity could be achieved.
32416684	10	77	theme	chitosan-coated	1298:1312	arg1	microparticles					1314:1327	The chitosan-coated microparticles	1294:1327	The chitosan-coated microparticles	1294:1327	The chitosan-coated microparticles were found to be more cytotoxic than uncoated microparticles and plain cisplatin solution.
32416684	10	77	theme	chitosan-coated	1298:1312	arg1	cytotoxic					1351:1359	cytotoxic	1351:1359	cytotoxic	1351:1359	The chitosan-coated microparticles were found to be more cytotoxic than uncoated microparticles and plain cisplatin solution.
32416684	0	78	theme	Microparticles	79:92	arg1	Optimization					10:21	Optimization	10:21	Optimization	10:21	DoE Based Optimization and Development of Spray-Dried Chitosan-Coated Alginate Microparticles Loaded with Cisplatin for the Treatment of Cervical Cancer.
32416684	0	78	theme	Microparticles	79:92	arg1	Development					27:37	Development	27:37	Development	27:37	DoE Based Optimization and Development of Spray-Dried Chitosan-Coated Alginate Microparticles Loaded with Cisplatin for the Treatment of Cervical Cancer.
32416684	7	79	theme	diffusion	1068:1076	arg1	cell					1078:1081	Franz diffusion cell	1062:1081	Franz diffusion cell	1062:1081	A drug permeability study was performed by Franz diffusion cell using the vaginal tissue of Swiss Albino Mice.
32416684	1	80	theme	existing	169:176	arg1	treatment					189:197	The existing parenteral treatment	165:197	The existing parenteral treatment of cervical cancer	165:216	BACKGROUND The existing parenteral treatment of cervical cancer has high toxicity and poor distribution of drugs at the targeted site.
32416684	4	81	theme	entrapment	778:787	arg1	size					755:758	Particle size	746:758	Particle size	746:758	The spray-dried uncoated and chitosan- coated microparticles were characterized for various parameters (Particle size, Morphology, Drug entrapment efficiency).
32416684	4	81	theme	entrapment	778:787	arg1	efficiency					789:798	Drug entrapment efficiency	773:798	Drug entrapment efficiency	773:798	The spray-dried uncoated and chitosan- coated microparticles were characterized for various parameters (Particle size, Morphology, Drug entrapment efficiency).
32416684	5	82	theme	drug	811:814	arg1	study					824:828	In vitro drug release study	802:828	In vitro drug release study	802:828	In vitro drug release study was carried out in simulated vaginal fluids by dialysis membrane method.
32416684	13	83	theme	mucoadhesive	1703:1714	arg1	microparticles					1728:1741	mucoadhesive spray-dried microparticles	1703:1741	mucoadhesive spray-dried microparticles	1703:1741	CONCLUSION Thus, it can be concluded that mucoadhesive spray-dried microparticles could provide a favorable approach for localized delivery of the anticancer drug via vaginal route against cervical cancer with its enhanced effectiveness.
32416684	7	84	theme	permeability	1026:1037	arg1	study					1039:1043	A drug permeability study	1019:1043	A drug permeability study	1019:1043	A drug permeability study was performed by Franz diffusion cell using the vaginal tissue of Swiss Albino Mice.
32416684	9	85	dep	in	1209:1210	arg1	vitro					1212:1216	vitro	1212:1216	vitro	1212:1216	The in vitro release studies indicated a controlled release following the Higuchi model.
32416684	13	86	theme	drug	1819:1822	arg1	delivery					1792:1799	localized delivery	1782:1799	localized delivery of the anticancer drug via vaginal route against cervical cancer with its enhanced effectiveness	1782:1896	CONCLUSION Thus, it can be concluded that mucoadhesive spray-dried microparticles could provide a favorable approach for localized delivery of the anticancer drug via vaginal route against cervical cancer with its enhanced effectiveness.
32416684	2	87	theme	therapeutics	425:436	arg1	benefits					438:445	enhanced therapeutics benefits	416:445	enhanced therapeutics benefits with low toxicity	416:463	PURPOSE To formulate localized mucoadhesive cisplatin loaded microparticles based formulation to treat cervical cancer so that enhanced therapeutics benefits with low toxicity could be achieved.
32416684	5	88	theme	membrane	886:893	arg1	method					895:900	dialysis membrane method	877:900	dialysis membrane method	877:900	In vitro drug release study was carried out in simulated vaginal fluids by dialysis membrane method.
32416684	5	89	theme	In	802:803	arg1	study					824:828	In vitro drug release study	802:828	In vitro drug release study	802:828	In vitro drug release study was carried out in simulated vaginal fluids by dialysis membrane method.
32416684	13	90	theme	vaginal	1828:1834	arg1	route					1836:1840	vaginal route	1828:1840	vaginal route against cervical cancer with its enhanced effectiveness	1828:1896	CONCLUSION Thus, it can be concluded that mucoadhesive spray-dried microparticles could provide a favorable approach for localized delivery of the anticancer drug via vaginal route against cervical cancer with its enhanced effectiveness.
32416684	10	91	theme	plain	1394:1398	arg1	solution					1410:1417	plain cisplatin solution	1394:1417	plain cisplatin solution	1394:1417	The chitosan-coated microparticles were found to be more cytotoxic than uncoated microparticles and plain cisplatin solution.
32416684	3	92	theme	coated	518:523	arg1	microparticles					537:550	Cisplatin loaded chitosan coated spray-dried microparticles	492:550	METHODS Cisplatin loaded chitosan coated spray-dried microparticles	484:550	METHODS Cisplatin loaded chitosan coated spray-dried microparticles were prepared by ionotropic gelation technique and optimized by Central Composite Design.
32416684	5	93	theme	vaginal	859:865	arg1	fluids					867:872	simulated vaginal fluids	849:872	simulated vaginal fluids	849:872	In vitro drug release study was carried out in simulated vaginal fluids by dialysis membrane method.
32416684	12	94	theme	tumor	1619:1623	arg1	volume					1625:1630	tumor volume	1619:1630	tumor volume	1619:1630	Finally, in vivo tumor regression and histopathological studies confirmed the significant decrease in tumor volume at different time intervals.
32416684	3	95	theme	loaded	502:507	arg1	microparticles					537:550	Cisplatin loaded chitosan coated spray-dried microparticles	492:550	METHODS Cisplatin loaded chitosan coated spray-dried microparticles	484:550	METHODS Cisplatin loaded chitosan coated spray-dried microparticles were prepared by ionotropic gelation technique and optimized by Central Composite Design.
32416684	0	96	theme	Chitosan-Coated	54:68	arg1	Microparticles					79:92	Spray-Dried Chitosan-Coated Alginate Microparticles	42:92	Spray-Dried Chitosan-Coated Alginate Microparticles Loaded with Cisplatin for the Treatment of Cervical Cancer	42:151	DoE Based Optimization and Development of Spray-Dried Chitosan-Coated Alginate Microparticles Loaded with Cisplatin for the Treatment of Cervical Cancer.
32416684	8	97	dep	RESULTS	1130:1136	arg1	found					1184:1188	found	1184:1188	were found to be optimum	1179:1202	RESULTS All in vitro characterization parameters were found to be optimum.
32416684	13	98	theme	favorable	1759:1767	arg1	approach					1769:1776	a favorable approach	1757:1776	a favorable approach for localized delivery of the anticancer drug via vaginal route against cervical cancer with its enhanced effectiveness	1757:1896	CONCLUSION Thus, it can be concluded that mucoadhesive spray-dried microparticles could provide a favorable approach for localized delivery of the anticancer drug via vaginal route against cervical cancer with its enhanced effectiveness.
32416684	11	99	theme	uncoated	1492:1499	arg1	microparticles					1501:1514	uncoated microparticles	1492:1514	uncoated microparticles	1492:1514	The chitosan-coated microparticles were found to be more permeable than uncoated microparticles.
32416684	1	100	theme	drugs	261:265	arg1	toxicity					227:234	high toxicity	222:234	high toxicity	222:234	BACKGROUND The existing parenteral treatment of cervical cancer has high toxicity and poor distribution of drugs at the targeted site.
32416684	1	100	theme	drugs	261:265	arg1	distribution					245:256	poor distribution	240:256	poor distribution	240:256	BACKGROUND The existing parenteral treatment of cervical cancer has high toxicity and poor distribution of drugs at the targeted site.
34364590	7	0	used	used	1095:1098	arg2	films					1080:1084	These films	1074:1084	These films	1074:1084	These films might be used to produce industrially useful coating material for food products.
34364590	3	1	theme	remarkable	448:457	arg1	loss					459:462	remarkable loss	448:462	remarkable loss of crystallinity in cellulose and starch due to fatty acid esterification	448:536	XRD study revealed remarkable loss of crystallinity in cellulose and starch due to fatty acid esterification.
34364590	5	2	theme	laminar	873:879	arg1	structures					881:890	The laminar structures	869:890	The laminar structures	869:890	The laminar structures also resulted significant reduction in mechanical strength of the composite films.
34364590	4	3	theme	vapor	815:819	arg1	barrier					821:827	water vapor barrier	809:827	water vapor barrier due to their non-layer microstructures	809:866	The addition of palmitate and stearate esters of cellulose and starch to AX formed laminar film microstructures which limited water vapor permeability whereas films prepared by blending AX with laurate and myristate esters of starch and cellulose were less effective as water vapor barrier due to their non-layer microstructures.
34364590	2	4	theme	stearic	320:326	arg1	acids					328:332	lauric, myristic, palmitic and stearic acids	289:332	lauric, myristic, palmitic and stearic acids	289:332	Thereafter, cellulose and starch were esterified with lauric, myristic, palmitic and stearic acids to prepare corresponding cellulose (CFAs) and starch fatty acid esters (SFAs) with DS 2.1-2.8.
34364590	3	5	from	loss	459:462	arg1	starch					498:503	starch	498:503	starch	498:503	XRD study revealed remarkable loss of crystallinity in cellulose and starch due to fatty acid esterification.
34364590	3	5	from	loss	459:462	arg1	cellulose					484:492	cellulose	484:492	cellulose	484:492	XRD study revealed remarkable loss of crystallinity in cellulose and starch due to fatty acid esterification.
34364590	1	6	dep	Arabinoxylan	123:134	arg1	AX					137:138	AX	137:138	AX	137:138	Arabinoxylan (AX) and cellulose were extracted from wheat straw, whereas starch was extracted from potato peel.
34364590	0	7	theme	physical	80:87	arg1	properties					89:98	physical properties	80:98	physical properties of arabinoxylan films	80:120	Effect of cellulose and starch fatty acid esters addition on microstructure and physical properties of arabinoxylan films.
34364590	4	8	theme	due	829:831	arg1	barrier					821:827	water vapor barrier	809:827	water vapor barrier due to their non-layer microstructures	809:866	The addition of palmitate and stearate esters of cellulose and starch to AX formed laminar film microstructures which limited water vapor permeability whereas films prepared by blending AX with laurate and myristate esters of starch and cellulose were less effective as water vapor barrier due to their non-layer microstructures.
34364590	3	9	theme	XRD	429:431	arg1	study					433:437	XRD study	429:437	XRD study	429:437	XRD study revealed remarkable loss of crystallinity in cellulose and starch due to fatty acid esterification.
34364590	0	10	theme	arabinoxylan	103:114	arg1	films					116:120	arabinoxylan films	103:120	arabinoxylan films	103:120	Effect of cellulose and starch fatty acid esters addition on microstructure and physical properties of arabinoxylan films.
34364590	5	11	theme	significant	906:916	arg1	reduction					918:926	significant reduction	906:926	significant reduction	906:926	The laminar structures also resulted significant reduction in mechanical strength of the composite films.
34364590	4	12	theme	esters	578:583	arg1	addition					543:550	The addition	539:550	The addition of palmitate and stearate esters of cellulose and starch to AX	539:613	The addition of palmitate and stearate esters of cellulose and starch to AX formed laminar film microstructures which limited water vapor permeability whereas films prepared by blending AX with laurate and myristate esters of starch and cellulose were less effective as water vapor barrier due to their non-layer microstructures.
34364590	4	13	theme	laurate	733:739	arg1	esters					755:760	laurate and myristate esters	733:760	laurate and myristate esters of starch and cellulose	733:784	The addition of palmitate and stearate esters of cellulose and starch to AX formed laminar film microstructures which limited water vapor permeability whereas films prepared by blending AX with laurate and myristate esters of starch and cellulose were less effective as water vapor barrier due to their non-layer microstructures.
34364590	6	14	theme	native	1050:1055	arg1	films					1067:1071	native composite films	1050:1071	native composite films	1050:1071	Furthermore, all AX-CFAs and AX-SFAs films were thermally more stable than native composite films.
34364590	4	15	theme	starch	602:607	arg1	esters					578:583	palmitate and stearate esters	555:583	palmitate and stearate esters of cellulose and starch	555:607	The addition of palmitate and stearate esters of cellulose and starch to AX formed laminar film microstructures which limited water vapor permeability whereas films prepared by blending AX with laurate and myristate esters of starch and cellulose were less effective as water vapor barrier due to their non-layer microstructures.
34364590	4	16	theme	water	809:813	arg1	barrier					821:827	water vapor barrier	809:827	water vapor barrier due to their non-layer microstructures	809:866	The addition of palmitate and stearate esters of cellulose and starch to AX formed laminar film microstructures which limited water vapor permeability whereas films prepared by blending AX with laurate and myristate esters of starch and cellulose were less effective as water vapor barrier due to their non-layer microstructures.
34364590	3	17	theme	fatty	512:516	arg1	acid					518:521	fatty acid	512:521	fatty acid esterification	512:536	XRD study revealed remarkable loss of crystallinity in cellulose and starch due to fatty acid esterification.
34364590	7	18	theme	food	1152:1155	arg1	products					1157:1164	food products	1152:1164	food products	1152:1164	These films might be used to produce industrially useful coating material for food products.
34364590	4	19	theme	vapor	671:675	arg1	permeability					677:688	water vapor permeability	665:688	water vapor permeability	665:688	The addition of palmitate and stearate esters of cellulose and starch to AX formed laminar film microstructures which limited water vapor permeability whereas films prepared by blending AX with laurate and myristate esters of starch and cellulose were less effective as water vapor barrier due to their non-layer microstructures.
34364590	2	20	theme	lauric	289:294	arg1	acids					328:332	lauric, myristic, palmitic and stearic acids	289:332	lauric, myristic, palmitic and stearic acids	289:332	Thereafter, cellulose and starch were esterified with lauric, myristic, palmitic and stearic acids to prepare corresponding cellulose (CFAs) and starch fatty acid esters (SFAs) with DS 2.1-2.8.
34364590	1	21	theme	wheat	175:179	arg1	straw					181:185	wheat straw	175:185	wheat straw	175:185	Arabinoxylan (AX) and cellulose were extracted from wheat straw, whereas starch was extracted from potato peel.
34364590	7	22	theme	coating	1131:1137	arg1	material					1139:1146	industrially useful coating material	1111:1146	industrially useful coating material for food products	1111:1164	These films might be used to produce industrially useful coating material for food products.
34364590	4	23	theme	cellulose	776:784	arg1	esters					755:760	laurate and myristate esters	733:760	laurate and myristate esters of starch and cellulose	733:784	The addition of palmitate and stearate esters of cellulose and starch to AX formed laminar film microstructures which limited water vapor permeability whereas films prepared by blending AX with laurate and myristate esters of starch and cellulose were less effective as water vapor barrier due to their non-layer microstructures.
34364590	3	24	theme	due	505:507	arg1	cellulose					484:492	cellulose	484:492	cellulose	484:492	XRD study revealed remarkable loss of crystallinity in cellulose and starch due to fatty acid esterification.
34364590	2	25	theme	fatty	387:391	arg1	SFAs					406:409	SFAs	406:409	SFAs	406:409	Thereafter, cellulose and starch were esterified with lauric, myristic, palmitic and stearic acids to prepare corresponding cellulose (CFAs) and starch fatty acid esters (SFAs) with DS 2.1-2.8.
34364590	2	25	theme	fatty	387:391	arg1	esters					398:403	starch fatty acid esters	380:403	starch fatty acid esters (SFAs)	380:410	Thereafter, cellulose and starch were esterified with lauric, myristic, palmitic and stearic acids to prepare corresponding cellulose (CFAs) and starch fatty acid esters (SFAs) with DS 2.1-2.8.
34364590	2	26	theme	acid	393:396	arg1	SFAs					406:409	SFAs	406:409	SFAs	406:409	Thereafter, cellulose and starch were esterified with lauric, myristic, palmitic and stearic acids to prepare corresponding cellulose (CFAs) and starch fatty acid esters (SFAs) with DS 2.1-2.8.
34364590	2	26	theme	acid	393:396	arg1	esters					398:403	starch fatty acid esters	380:403	starch fatty acid esters (SFAs)	380:410	Thereafter, cellulose and starch were esterified with lauric, myristic, palmitic and stearic acids to prepare corresponding cellulose (CFAs) and starch fatty acid esters (SFAs) with DS 2.1-2.8.
34364590	4	27	with	AX	725:726	arg1	esters					755:760	laurate and myristate esters	733:760	laurate and myristate esters of starch and cellulose	733:784	The addition of palmitate and stearate esters of cellulose and starch to AX formed laminar film microstructures which limited water vapor permeability whereas films prepared by blending AX with laurate and myristate esters of starch and cellulose were less effective as water vapor barrier due to their non-layer microstructures.
34364590	0	28	theme	films	116:120	arg1	microstructure					61:74	microstructure	61:74	microstructure	61:74	Effect of cellulose and starch fatty acid esters addition on microstructure and physical properties of arabinoxylan films.
34364590	0	28	theme	films	116:120	arg1	properties					89:98	physical properties	80:98	physical properties of arabinoxylan films	80:120	Effect of cellulose and starch fatty acid esters addition on microstructure and physical properties of arabinoxylan films.
34364590	6	29	theme	composite	1057:1065	arg1	films					1067:1071	native composite films	1050:1071	native composite films	1050:1071	Furthermore, all AX-CFAs and AX-SFAs films were thermally more stable than native composite films.
34364590	2	30	theme	starch	380:385	arg1	SFAs					406:409	SFAs	406:409	SFAs	406:409	Thereafter, cellulose and starch were esterified with lauric, myristic, palmitic and stearic acids to prepare corresponding cellulose (CFAs) and starch fatty acid esters (SFAs) with DS 2.1-2.8.
34364590	2	30	theme	starch	380:385	arg1	esters					398:403	starch fatty acid esters	380:403	starch fatty acid esters (SFAs)	380:410	Thereafter, cellulose and starch were esterified with lauric, myristic, palmitic and stearic acids to prepare corresponding cellulose (CFAs) and starch fatty acid esters (SFAs) with DS 2.1-2.8.
34364590	0	31	theme	fatty	31:35	arg1	esters					42:47	starch fatty acid esters	24:47	starch fatty acid esters	24:47	Effect of cellulose and starch fatty acid esters addition on microstructure and physical properties of arabinoxylan films.
34364590	5	32	theme	mechanical	931:940	arg1	strength					942:949	mechanical strength	931:949	mechanical strength of the composite films	931:972	The laminar structures also resulted significant reduction in mechanical strength of the composite films.
34364590	2	33	theme	corresponding	345:357	arg1	CFAs					370:373	CFAs	370:373	CFAs	370:373	Thereafter, cellulose and starch were esterified with lauric, myristic, palmitic and stearic acids to prepare corresponding cellulose (CFAs) and starch fatty acid esters (SFAs) with DS 2.1-2.8.
34364590	2	33	theme	corresponding	345:357	arg1	cellulose					359:367	corresponding cellulose	345:367	corresponding cellulose (CFAs)	345:374	Thereafter, cellulose and starch were esterified with lauric, myristic, palmitic and stearic acids to prepare corresponding cellulose (CFAs) and starch fatty acid esters (SFAs) with DS 2.1-2.8.
34364590	3	34	theme	acid	518:521	arg1	esterification					523:536	fatty acid esterification	512:536	fatty acid esterification	512:536	XRD study revealed remarkable loss of crystallinity in cellulose and starch due to fatty acid esterification.
34364590	0	35	theme	starch	24:29	arg1	esters					42:47	starch fatty acid esters	24:47	starch fatty acid esters	24:47	Effect of cellulose and starch fatty acid esters addition on microstructure and physical properties of arabinoxylan films.
34364590	7	36	theme	useful	1124:1129	arg1	material					1139:1146	industrially useful coating material	1111:1146	industrially useful coating material for food products	1111:1164	These films might be used to produce industrially useful coating material for food products.
34364590	4	37	theme	starch	765:770	arg1	esters					755:760	laurate and myristate esters	733:760	laurate and myristate esters of starch and cellulose	733:784	The addition of palmitate and stearate esters of cellulose and starch to AX formed laminar film microstructures which limited water vapor permeability whereas films prepared by blending AX with laurate and myristate esters of starch and cellulose were less effective as water vapor barrier due to their non-layer microstructures.
34364590	4	38	theme	cellulose	588:596	arg1	esters					578:583	palmitate and stearate esters	555:583	palmitate and stearate esters of cellulose and starch	555:607	The addition of palmitate and stearate esters of cellulose and starch to AX formed laminar film microstructures which limited water vapor permeability whereas films prepared by blending AX with laurate and myristate esters of starch and cellulose were less effective as water vapor barrier due to their non-layer microstructures.
34364590	4	39	theme	non-layer	842:850	arg1	microstructures					852:866	their non-layer microstructures	836:866	their non-layer microstructures	836:866	The addition of palmitate and stearate esters of cellulose and starch to AX formed laminar film microstructures which limited water vapor permeability whereas films prepared by blending AX with laurate and myristate esters of starch and cellulose were less effective as water vapor barrier due to their non-layer microstructures.
34364590	0	40	from	addition	49:56	arg1	microstructure					61:74	microstructure	61:74	microstructure	61:74	Effect of cellulose and starch fatty acid esters addition on microstructure and physical properties of arabinoxylan films.
34364590	0	40	from	addition	49:56	arg1	properties					89:98	physical properties	80:98	physical properties of arabinoxylan films	80:120	Effect of cellulose and starch fatty acid esters addition on microstructure and physical properties of arabinoxylan films.
34364590	4	41	theme	water	665:669	arg1	permeability					677:688	water vapor permeability	665:688	water vapor permeability	665:688	The addition of palmitate and stearate esters of cellulose and starch to AX formed laminar film microstructures which limited water vapor permeability whereas films prepared by blending AX with laurate and myristate esters of starch and cellulose were less effective as water vapor barrier due to their non-layer microstructures.
34364590	0	42	theme	acid	37:40	arg1	esters					42:47	starch fatty acid esters	24:47	starch fatty acid esters	24:47	Effect of cellulose and starch fatty acid esters addition on microstructure and physical properties of arabinoxylan films.
34364590	1	43	dep	extracted	160:168	arg1	whereas					188:194	whereas	188:194	whereas	188:194	Arabinoxylan (AX) and cellulose were extracted from wheat straw, whereas starch was extracted from potato peel.
34364590	2	44	theme	palmitic	307:314	arg1	acids					328:332	lauric, myristic, palmitic and stearic acids	289:332	lauric, myristic, palmitic and stearic acids	289:332	Thereafter, cellulose and starch were esterified with lauric, myristic, palmitic and stearic acids to prepare corresponding cellulose (CFAs) and starch fatty acid esters (SFAs) with DS 2.1-2.8.
34364590	5	45	theme	composite	958:966	arg1	films					968:972	the composite films	954:972	the composite films	954:972	The laminar structures also resulted significant reduction in mechanical strength of the composite films.
34364590	4	46	theme	myristate	745:753	arg1	esters					755:760	laurate and myristate esters	733:760	laurate and myristate esters of starch and cellulose	733:784	The addition of palmitate and stearate esters of cellulose and starch to AX formed laminar film microstructures which limited water vapor permeability whereas films prepared by blending AX with laurate and myristate esters of starch and cellulose were less effective as water vapor barrier due to their non-layer microstructures.
34364590	4	47	theme	laminar	622:628	arg1	microstructures					635:649	laminar film microstructures	622:649	laminar film microstructures which limited water vapor permeability whereas films prepared by blending AX with laurate and myristate esters of starch and cellulose were less effective as water vapor barrier due to their non-layer microstructures	622:866	The addition of palmitate and stearate esters of cellulose and starch to AX formed laminar film microstructures which limited water vapor permeability whereas films prepared by blending AX with laurate and myristate esters of starch and cellulose were less effective as water vapor barrier due to their non-layer microstructures.
34364590	4	48	theme	palmitate	555:563	arg1	esters					578:583	palmitate and stearate esters	555:583	palmitate and stearate esters of cellulose and starch	555:607	The addition of palmitate and stearate esters of cellulose and starch to AX formed laminar film microstructures which limited water vapor permeability whereas films prepared by blending AX with laurate and myristate esters of starch and cellulose were less effective as water vapor barrier due to their non-layer microstructures.
34364590	5	49	theme	films	968:972	arg1	strength					942:949	mechanical strength	931:949	mechanical strength of the composite films	931:972	The laminar structures also resulted significant reduction in mechanical strength of the composite films.
34364590	2	50	theme	myristic	297:304	arg1	acids					328:332	lauric, myristic, palmitic and stearic acids	289:332	lauric, myristic, palmitic and stearic acids	289:332	Thereafter, cellulose and starch were esterified with lauric, myristic, palmitic and stearic acids to prepare corresponding cellulose (CFAs) and starch fatty acid esters (SFAs) with DS 2.1-2.8.
34364590	6	51	theme	AX-CFAs	992:998	arg1	films					1012:1016	all AX-CFAs and AX-SFAs films	988:1016	all AX-CFAs and AX-SFAs films	988:1016	Furthermore, all AX-CFAs and AX-SFAs films were thermally more stable than native composite films.
34364590	6	52	theme	AX-SFAs	1004:1010	arg1	films					1012:1016	all AX-CFAs and AX-SFAs films	988:1016	all AX-CFAs and AX-SFAs films	988:1016	Furthermore, all AX-CFAs and AX-SFAs films were thermally more stable than native composite films.
34364590	4	53	theme	film	630:633	arg1	microstructures					635:649	laminar film microstructures	622:649	laminar film microstructures which limited water vapor permeability whereas films prepared by blending AX with laurate and myristate esters of starch and cellulose were less effective as water vapor barrier due to their non-layer microstructures	622:866	The addition of palmitate and stearate esters of cellulose and starch to AX formed laminar film microstructures which limited water vapor permeability whereas films prepared by blending AX with laurate and myristate esters of starch and cellulose were less effective as water vapor barrier due to their non-layer microstructures.
34364590	4	54	theme	stearate	569:576	arg1	esters					578:583	palmitate and stearate esters	555:583	palmitate and stearate esters of cellulose and starch	555:607	The addition of palmitate and stearate esters of cellulose and starch to AX formed laminar film microstructures which limited water vapor permeability whereas films prepared by blending AX with laurate and myristate esters of starch and cellulose were less effective as water vapor barrier due to their non-layer microstructures.
34364590	0	55	theme	cellulose	10:18	arg1	Effect					0:5	Effect	0:5	Effect of cellulose	0:18	Effect of cellulose and starch fatty acid esters addition on microstructure and physical properties of arabinoxylan films.
34364590	0	55	theme	cellulose	10:18	arg1	esters					42:47	starch fatty acid esters	24:47	starch fatty acid esters	24:47	Effect of cellulose and starch fatty acid esters addition on microstructure and physical properties of arabinoxylan films.
34364590	3	56	theme	crystallinity	467:479	arg1	loss					459:462	remarkable loss	448:462	remarkable loss of crystallinity in cellulose and starch due to fatty acid esterification	448:536	XRD study revealed remarkable loss of crystallinity in cellulose and starch due to fatty acid esterification.
34364590	1	57	theme	potato	222:227	arg1	peel					229:232	potato peel	222:232	potato peel	222:232	Arabinoxylan (AX) and cellulose were extracted from wheat straw, whereas starch was extracted from potato peel.
33978738	5	0	dep	annotation	1429:1438	arg1	iv					1425:1426	iv	1425:1426	iv	1425:1426	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	3	1	from	present	562:568	arg1	structures					594:603	more than 14,000 PDB structures	573:603	more than 14,000 PDB structures	573:603	Carbohydrate molecules present in more than 14,000 PDB structures have recently been reviewed and remediated to conform to a new standardized format.
33978738	3	2	theme	PDB	590:592	arg1	structures					594:603	more than 14,000 PDB structures	573:603	more than 14,000 PDB structures	573:603	Carbohydrate molecules present in more than 14,000 PDB structures have recently been reviewed and remediated to conform to a new standardized format.
33978738	4	3	theme	data	711:714	arg1	representation					716:729	This machine-readable data representation	689:729	This machine-readable data representation for carbohydrates occurring in the PDB structures and the corresponding reference data	689:816	This machine-readable data representation for carbohydrates occurring in the PDB structures and the corresponding reference data improves the findability, accessibility, interoperability and reusability of structural information pertaining to these molecules.
33978738	5	4	gly	glycosylation	1443:1455	arg2	sites					1457:1461	glycosylation sites	1443:1461	glycosylation sites in proteins	1443:1473	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	6	5	theme	structure	1705:1713	arg1	visualization					1690:1702	improved carbohydrate visualization	1668:1702	improved carbohydrate visualization	1668:1702	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	6	5	theme	structure	1705:1713	arg1	validation					1715:1724	structure validation	1705:1724	structure validation	1705:1724	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	5	6	theme	PDB	953:955	arg1	data					1015:1018	The PDB Exchange MacroMolecular Crystallographic Information File data	949:1018	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary	949:1029	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	1	7	theme	global	151:156	arg1	Bank					115:118	the Protein Data Bank	98:118	the Protein Data Bank (PDB)	98:124	Since 1971, the Protein Data Bank (PDB) has served as the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability.
33978738	1	7	theme	global	151:156	arg1	archive					158:164	the single global archive	140:164	the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability	140:374	Since 1971, the Protein Data Bank (PDB) has served as the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability.
33978738	7	8	theme	glycoscience	1959:1970	arg1	community					1972:1980	the glycoscience community and researchers	1955:1996	community	1972:1980	The uniform representation of carbohydrate molecules in the PDB described herein will facilitate broader usage of the resource by the glycoscience community and researchers studying glycoproteins.
33978738	5	9	from	community	1410:1418	arg1	proteins					1466:1473	proteins	1466:1473	proteins	1466:1473	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	5	10	theme	Biology	1196:1202	arg1	recommendations					1218:1232	Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations	1169:1232	Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates	1169:1250	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	5	10	theme	Biology	1196:1202	arg1	representation					1266:1279	(ii) uniform representation	1253:1279	(ii) uniform representation of branched entities for oligosaccharides	1253:1321	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	6	11	theme	improved	1668:1675	arg1	validation					1715:1724	structure validation	1705:1724	structure validation	1705:1724	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	6	11	theme	improved	1668:1675	arg1	visualization					1690:1702	improved carbohydrate visualization	1668:1702	improved carbohydrate visualization	1668:1702	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	6	11	theme	improved	1668:1675	arg1	search					1773:1778	search	1773:1778	search for dendritic structures and classification	1773:1822	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	6	11	theme	improved	1668:1675	arg1	analyses					1763:1770	robust quantitative and qualitative analyses	1727:1770	robust quantitative and qualitative analyses	1727:1770	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	1	12	theme	determined	185:194	arg1	structures					199:208	experimentally determined 3D structures	170:208	experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability	170:374	Since 1971, the Protein Data Bank (PDB) has served as the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability.
33978738	5	13	dep	conforms	1084:1091	arg1	used					1339:1342	used	1339:1342	used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins	1339:1473	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	1	14	theme	Data	110:113	arg1	Bank					115:118	the Protein Data Bank	98:118	the Protein Data Bank (PDB)	98:124	Since 1971, the Protein Data Bank (PDB) has served as the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability.
33978738	1	14	theme	Data	110:113	arg1	PDB					121:123	PDB	121:123	PDB	121:123	Since 1971, the Protein Data Bank (PDB) has served as the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability.
33978738	1	14	theme	Data	110:113	arg1	archive					158:164	the single global archive	140:164	the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability	140:374	Since 1971, the Protein Data Bank (PDB) has served as the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability.
33978738	7	15	theme	resource	1943:1950	arg1	usage					1930:1934	broader usage	1922:1934	broader usage of the resource by the glycoscience community and researchers studying glycoproteins	1922:2019	The uniform representation of carbohydrate molecules in the PDB described herein will facilitate broader usage of the resource by the glycoscience community and researchers studying glycoproteins.
33978738	5	16	dep	used	1339:1342	arg1	iii					1325:1327	iii	1325:1327	iii	1325:1327	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	5	17	from	proteins	1466:1473	arg1	annotation					1429:1438	(iv) annotation	1424:1438	(iv) annotation	1424:1438	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	5	17	from	proteins	1466:1473	arg1	community					1410:1418	the glycoscience community	1393:1418	the glycoscience community	1393:1418	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	5	18	dep	nomenclature	1066:1077	arg1	i					1045:1045	i	1045:1045	i	1045:1045	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	3	19	theme	new	664:666	arg1	format					681:686	a new standardized format	662:686	a new standardized format	662:686	Carbohydrate molecules present in more than 14,000 PDB structures have recently been reviewed and remediated to conform to a new standardized format.
33978738	6	20	theme	qualitative	1751:1761	arg1	visualization					1690:1702	improved carbohydrate visualization	1668:1702	improved carbohydrate visualization	1668:1702	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	6	20	theme	qualitative	1751:1761	arg1	analyses					1763:1770	robust quantitative and qualitative analyses	1727:1770	robust quantitative and qualitative analyses	1727:1770	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	5	21	from	sites	1457:1461	arg1	proteins					1466:1473	proteins	1466:1473	proteins	1466:1473	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	0	22	theme	Protein	67:73	arg1	Bank					80:83	the Protein Data Bank	63:83	the Protein Data Bank	63:83	Modernized uniform representation of carbohydrate molecules in the Protein Data Bank.
33978738	6	23	from	carbohydrates	1496:1508	arg1	structures					1517:1526	PDB structures	1513:1526	PDB structures	1513:1526	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	6	24	theme	quantitative	1734:1745	arg1	visualization					1690:1702	improved carbohydrate visualization	1668:1702	improved carbohydrate visualization	1668:1702	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	6	24	theme	quantitative	1734:1745	arg1	analyses					1763:1770	robust quantitative and qualitative analyses	1727:1770	robust quantitative and qualitative analyses	1727:1770	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	5	25	theme	Information	998:1008	arg1	data					1015:1018	The PDB Exchange MacroMolecular Crystallographic Information File data	949:1018	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary	949:1029	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	7	26	theme	broader	1922:1928	arg1	usage					1930:1934	broader usage	1922:1934	broader usage of the resource by the glycoscience community and researchers studying glycoproteins	1922:2019	The uniform representation of carbohydrate molecules in the PDB described herein will facilitate broader usage of the resource by the glycoscience community and researchers studying glycoproteins.
33978738	2	27	theme	biological	517:526	arg1	phenomena					528:536	rich and complex biological phenomena	500:536	rich and complex biological phenomena	500:536	During the first 50 years of continuous PDB operations, standards for data representation have evolved to better represent rich and complex biological phenomena.
33978738	0	28	from	representation	19:32	arg1	Bank					80:83	the Protein Data Bank	63:83	the Protein Data Bank	63:83	Modernized uniform representation of carbohydrate molecules in the Protein Data Bank.
33978738	1	29	theme	Findability-Accessibility-Interoperability-Reusability	321:374	arg1	principles					307:316	the FAIR principles	298:316	the FAIR principles of Findability-Accessibility-Interoperability-Reusability	298:374	Since 1971, the Protein Data Bank (PDB) has served as the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability.
33978738	5	30	theme	data	1015:1018	arg1	dictionary					1020:1029	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary	949:1029	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary	949:1029	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	7	31	gly	glycoproteins	2007:2019	arg1	glycoproteins					2007:2019	glycoproteins	2007:2019	glycoproteins	2007:2019	The uniform representation of carbohydrate molecules in the PDB described herein will facilitate broader usage of the resource by the glycoscience community and researchers studying glycoproteins.
33978738	5	32	theme	glycoscience	1397:1408	arg1	community					1410:1418	the glycoscience community	1393:1418	the glycoscience community	1393:1418	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	6	33	theme	first	1484:1488	arg1	time					1490:1493	the first time	1480:1493	the first time	1480:1493	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	5	34	theme	recommendations	1218:1232	arg1	Union					1160:1164	Pure and Applied Chemistry-International Union	1119:1164	Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides	1119:1321	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	2	35	theme	operations	421:430	arg1	50 years					394:401	the first 50 years	384:401	the first 50 years of continuous PDB operations	384:430	During the first 50 years of continuous PDB operations, standards for data representation have evolved to better represent rich and complex biological phenomena.
33978738	0	36	theme	Data	75:78	arg1	Bank					80:83	the Protein Data Bank	63:83	the Protein Data Bank	63:83	Modernized uniform representation of carbohydrate molecules in the Protein Data Bank.
33978738	4	37	theme	reference	803:811	arg1	data					813:816	the corresponding reference data	785:816	the corresponding reference data	785:816	This machine-readable data representation for carbohydrates occurring in the PDB structures and the corresponding reference data improves the findability, accessibility, interoperability and reusability of structural information pertaining to these molecules.
33978738	2	38	theme	continuous	406:415	arg1	operations					421:430	continuous PDB operations	406:430	continuous PDB operations	406:430	During the first 50 years of continuous PDB operations, standards for data representation have evolved to better represent rich and complex biological phenomena.
33978738	7	39	from	representation	1837:1850	arg1	PDB					1885:1887	the PDB	1881:1887	the PDB described herein	1881:1904	The uniform representation of carbohydrate molecules in the PDB described herein will facilitate broader usage of the resource by the glycoscience community and researchers studying glycoproteins.
33978738	7	40	theme	molecules	1868:1876	arg1	representation					1837:1850	The uniform representation	1825:1850	The uniform representation of carbohydrate molecules in the PDB described herein	1825:1904	The uniform representation of carbohydrate molecules in the PDB described herein will facilitate broader usage of the resource by the glycoscience community and researchers studying glycoproteins.
33978738	2	41	theme	data	447:450	arg1	representation					452:465	data representation	447:465	data representation	447:465	During the first 50 years of continuous PDB operations, standards for data representation have evolved to better represent rich and complex biological phenomena.
33978738	1	42	theme	global	268:273	arg1	community					275:283	the global community	264:283	the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability	264:374	Since 1971, the Protein Data Bank (PDB) has served as the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability.
33978738	5	43	theme	atom	1061:1064	arg1	nomenclature					1066:1077	(i) standardized atom nomenclature	1044:1077	(i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins	1044:1473	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	4	44	theme	structural	895:904	arg1	information					906:916	structural information	895:916	structural information pertaining to these molecules	895:946	This machine-readable data representation for carbohydrates occurring in the PDB structures and the corresponding reference data improves the findability, accessibility, interoperability and reusability of structural information pertaining to these molecules.
33978738	5	45	theme	glycosylation	1443:1455	arg1	sites					1457:1461	glycosylation sites	1443:1461	glycosylation sites in proteins	1443:1473	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	0	46	theme	carbohydrate	37:48	arg1	molecules					50:58	carbohydrate molecules	37:58	carbohydrate molecules	37:58	Modernized uniform representation of carbohydrate molecules in the Protein Data Bank.
33978738	5	47	theme	Applied	1128:1134	arg1	Union					1160:1164	Pure and Applied Chemistry-International Union	1119:1164	Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides	1119:1321	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	5	48	theme	branched	1284:1291	arg1	entities					1293:1300	branched entities	1284:1300	branched entities	1284:1300	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	5	49	theme	linear	1344:1349	arg1	descriptors					1351:1361	linear descriptors	1344:1361	linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins	1344:1473	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	3	50	theme	present	562:568	arg1	molecules					552:560	Carbohydrate molecules	539:560	Carbohydrate molecules present in more than 14,000 PDB structures	539:603	Carbohydrate molecules present in more than 14,000 PDB structures have recently been reviewed and remediated to conform to a new standardized format.
33978738	5	51	theme	Pure	1119:1122	arg1	Union					1160:1164	Pure and Applied Chemistry-International Union	1119:1164	Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides	1119:1321	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	3	52	theme	Carbohydrate	539:550	arg1	molecules					552:560	Carbohydrate molecules	539:560	Carbohydrate molecules present in more than 14,000 PDB structures	539:603	Carbohydrate molecules present in more than 14,000 PDB structures have recently been reviewed and remediated to conform to a new standardized format.
33978738	1	53	theme	macromolecules	224:237	arg1	structures					199:208	experimentally determined 3D structures	170:208	experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability	170:374	Since 1971, the Protein Data Bank (PDB) has served as the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability.
33978738	6	54	theme	monosaccharides	1588:1602	arg1	collections					1560:1570	collections	1560:1570	collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification	1560:1822	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	6	54	theme	monosaccharides	1588:1602	arg1	carbohydrates					1496:1508	carbohydrates	1496:1508	carbohydrates in PDB structures	1496:1526	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	4	55	dep	findability	831:841	arg1	the					827:829	the	827:829	the	827:829	This machine-readable data representation for carbohydrates occurring in the PDB structures and the corresponding reference data improves the findability, accessibility, interoperability and reusability of structural information pertaining to these molecules.
33978738	5	56	theme	Exchange	957:964	arg1	data					1015:1018	The PDB Exchange MacroMolecular Crystallographic Information File data	949:1018	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary	949:1029	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	4	57	theme	machine-readable	694:709	arg1	representation					716:729	This machine-readable data representation	689:729	This machine-readable data representation for carbohydrates occurring in the PDB structures and the corresponding reference data	689:816	This machine-readable data representation for carbohydrates occurring in the PDB structures and the corresponding reference data improves the findability, accessibility, interoperability and reusability of structural information pertaining to these molecules.
33978738	1	58	theme	single	144:149	arg1	Bank					115:118	the Protein Data Bank	98:118	the Protein Data Bank (PDB)	98:124	Since 1971, the Protein Data Bank (PDB) has served as the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability.
33978738	1	58	theme	single	144:149	arg1	archive					158:164	the single global archive	140:164	the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability	140:374	Since 1971, the Protein Data Bank (PDB) has served as the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability.
33978738	7	59	theme	uniform	1829:1835	arg1	representation					1837:1850	The uniform representation	1825:1850	The uniform representation of carbohydrate molecules in the PDB described herein	1825:1904	The uniform representation of carbohydrate molecules in the PDB described herein will facilitate broader usage of the resource by the glycoscience community and researchers studying glycoproteins.
33978738	6	60	theme	carbohydrate	1677:1688	arg1	validation					1715:1724	structure validation	1705:1724	structure validation	1705:1724	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	6	60	theme	carbohydrate	1677:1688	arg1	visualization					1690:1702	improved carbohydrate visualization	1668:1702	improved carbohydrate visualization	1668:1702	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	6	60	theme	carbohydrate	1677:1688	arg1	search					1773:1778	search	1773:1778	search for dendritic structures and classification	1773:1822	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	6	60	theme	carbohydrate	1677:1688	arg1	analyses					1763:1770	robust quantitative and qualitative analyses	1727:1770	robust quantitative and qualitative analyses	1727:1770	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	5	61	theme	MacroMolecular	966:979	arg1	data					1015:1018	The PDB Exchange MacroMolecular Crystallographic Information File data	949:1018	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary	949:1029	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	1	62	theme	FAIR	302:305	arg1	principles					307:316	the FAIR principles	298:316	the FAIR principles of Findability-Accessibility-Interoperability-Reusability	298:374	Since 1971, the Protein Data Bank (PDB) has served as the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability.
33978738	1	63	theme	Protein	102:108	arg1	Bank					115:118	the Protein Data Bank	98:118	the Protein Data Bank (PDB)	98:124	Since 1971, the Protein Data Bank (PDB) has served as the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability.
33978738	1	63	theme	Protein	102:108	arg1	PDB					121:123	PDB	121:123	PDB	121:123	Since 1971, the Protein Data Bank (PDB) has served as the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability.
33978738	1	63	theme	Protein	102:108	arg1	archive					158:164	the single global archive	140:164	the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability	140:374	Since 1971, the Protein Data Bank (PDB) has served as the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability.
33978738	5	64	from	annotation	1429:1438	arg1	proteins					1466:1473	proteins	1466:1473	proteins	1466:1473	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	4	65	theme	PDB	766:768	arg1	structures					770:779	the PDB structures	762:779	the PDB structures	762:779	This machine-readable data representation for carbohydrates occurring in the PDB structures and the corresponding reference data improves the findability, accessibility, interoperability and reusability of structural information pertaining to these molecules.
33978738	3	66	from	structures	594:603	arg1	present					562:568	present	562:568	present	562:568	Carbohydrate molecules present in more than 14,000 PDB structures have recently been reviewed and remediated to conform to a new standardized format.
33978738	5	67	theme	Biochemistry	1169:1180	arg1	recommendations					1218:1232	Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations	1169:1232	Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates	1169:1250	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	5	67	theme	Biochemistry	1169:1180	arg1	representation					1266:1279	(ii) uniform representation	1253:1279	(ii) uniform representation of branched entities for oligosaccharides	1253:1321	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	6	68	theme	PDB	1513:1515	arg1	structures					1517:1526	PDB structures	1513:1526	PDB structures	1513:1526	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	5	69	theme	Molecular	1186:1194	arg1	Biology					1196:1202	Molecular Biology	1186:1202	Molecular Biology	1186:1202	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	5	70	theme	Crystallographic	981:996	arg1	data					1015:1018	The PDB Exchange MacroMolecular Crystallographic Information File data	949:1018	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary	949:1029	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	1	71	theme	3D	196:197	arg1	structures					199:208	experimentally determined 3D structures	170:208	experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability	170:374	Since 1971, the Protein Data Bank (PDB) has served as the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability.
33978738	6	72	theme	robust	1727:1732	arg1	visualization					1690:1702	improved carbohydrate visualization	1668:1702	improved carbohydrate visualization	1668:1702	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	6	72	theme	robust	1727:1732	arg1	analyses					1763:1770	robust quantitative and qualitative analyses	1727:1770	robust quantitative and qualitative analyses	1727:1770	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	5	73	theme	File	1010:1013	arg1	data					1015:1018	The PDB Exchange MacroMolecular Crystallographic Information File data	949:1018	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary	949:1029	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	2	74	theme	complex	509:515	arg1	phenomena					528:536	rich and complex biological phenomena	500:536	rich and complex biological phenomena	500:536	During the first 50 years of continuous PDB operations, standards for data representation have evolved to better represent rich and complex biological phenomena.
33978738	5	75	theme	uniform	1258:1264	arg1	representation					1266:1279	(ii) uniform representation	1253:1279	(ii) uniform representation of branched entities for oligosaccharides	1253:1321	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	5	75	theme	uniform	1258:1264	arg1	recommendations					1218:1232	Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations	1169:1232	Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates	1169:1250	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	5	76	theme	sites	1457:1461	arg1	annotation					1429:1438	(iv) annotation	1424:1438	(iv) annotation	1424:1438	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	5	76	theme	sites	1457:1461	arg1	community					1410:1418	the glycoscience community	1393:1418	the glycoscience community	1393:1418	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	5	77	dep	representation	1266:1279	arg1	ii					1254:1255	ii	1254:1255	ii	1254:1255	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	2	78	theme	PDB	417:419	arg1	operations					421:430	continuous PDB operations	406:430	continuous PDB operations	406:430	During the first 50 years of continuous PDB operations, standards for data representation have evolved to better represent rich and complex biological phenomena.
33978738	0	79	theme	uniform	11:17	arg1	representation					19:32	uniform representation	11:32	uniform representation of carbohydrate molecules in the Protein Data Bank	11:83	Modernized uniform representation of carbohydrate molecules in the Protein Data Bank.
33978738	4	80	theme	corresponding	789:801	arg1	data					813:816	the corresponding reference data	785:816	the corresponding reference data	785:816	This machine-readable data representation for carbohydrates occurring in the PDB structures and the corresponding reference data improves the findability, accessibility, interoperability and reusability of structural information pertaining to these molecules.
33978738	6	81	theme	dendritic	1784:1792	arg1	structures					1794:1803	dendritic structures	1784:1803	dendritic structures	1784:1803	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	5	82	theme	standardized	1048:1059	arg1	nomenclature					1066:1077	(i) standardized atom nomenclature	1044:1077	(i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins	1044:1473	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	0	83	theme	molecules	50:58	arg1	representation					19:32	uniform representation	11:32	uniform representation of carbohydrate molecules in the Protein Data Bank	11:83	Modernized uniform representation of carbohydrate molecules in the Protein Data Bank.
33978738	7	84	theme	carbohydrate	1855:1866	arg1	molecules					1868:1876	carbohydrate molecules	1855:1876	carbohydrate molecules	1855:1876	The uniform representation of carbohydrate molecules in the PDB described herein will facilitate broader usage of the resource by the glycoscience community and researchers studying glycoproteins.
33978738	2	85	theme	rich	500:503	arg1	phenomena					528:536	rich and complex biological phenomena	500:536	rich and complex biological phenomena	500:536	During the first 50 years of continuous PDB operations, standards for data representation have evolved to better represent rich and complex biological phenomena.
33978738	3	86	theme	standardized	668:679	arg1	format					681:686	a new standardized format	662:686	a new standardized format	662:686	Carbohydrate molecules present in more than 14,000 PDB structures have recently been reviewed and remediated to conform to a new standardized format.
33978738	5	87	theme	Chemistry-International	1136:1158	arg1	Union					1160:1164	Pure and Applied Chemistry-International Union	1119:1164	Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides	1119:1321	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	3	88	attach	present	562:568	arg2	molecules					552:560	Carbohydrate molecules	539:560	Carbohydrate molecules present in more than 14,000 PDB structures	539:603	Carbohydrate molecules present in more than 14,000 PDB structures have recently been reviewed and remediated to conform to a new standardized format.
33978738	3	88	attach	present	562:568	arg1	structures					594:603	more than 14,000 PDB structures	573:603	more than 14,000 PDB structures	573:603	Carbohydrate molecules present in more than 14,000 PDB structures have recently been reviewed and remediated to conform to a new standardized format.
33978738	4	89	theme	information	906:916	arg1	findability					831:841	findability	831:841	findability	831:841	This machine-readable data representation for carbohydrates occurring in the PDB structures and the corresponding reference data improves the findability, accessibility, interoperability and reusability of structural information pertaining to these molecules.
33978738	4	89	theme	information	906:916	arg1	reusability					880:890	reusability	880:890	reusability	880:890	This machine-readable data representation for carbohydrates occurring in the PDB structures and the corresponding reference data improves the findability, accessibility, interoperability and reusability of structural information pertaining to these molecules.
33978738	4	89	theme	information	906:916	arg1	interoperability					859:874	interoperability	859:874	interoperability	859:874	This machine-readable data representation for carbohydrates occurring in the PDB structures and the corresponding reference data improves the findability, accessibility, interoperability and reusability of structural information pertaining to these molecules.
33978738	4	89	theme	information	906:916	arg1	accessibility					844:856	accessibility	844:856	accessibility	844:856	This machine-readable data representation for carbohydrates occurring in the PDB structures and the corresponding reference data improves the findability, accessibility, interoperability and reusability of structural information pertaining to these molecules.
33978738	5	90	theme	entities	1293:1300	arg1	representation					1266:1279	(ii) uniform representation	1253:1279	(ii) uniform representation of branched entities for oligosaccharides	1253:1321	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	5	90	theme	entities	1293:1300	arg1	recommendations					1218:1232	Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations	1169:1232	Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates	1169:1250	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33978738	2	91	theme	first	388:392	arg1	50 years					394:401	the first 50 years	384:401	the first 50 years of continuous PDB operations	384:430	During the first 50 years of continuous PDB operations, standards for data representation have evolved to better represent rich and complex biological phenomena.
33978738	6	92	theme	oligosaccharide	1630:1644	arg1	structures					1646:1655	oligosaccharide structures	1630:1655	oligosaccharide structures	1630:1655	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	1	93	theme	biological	213:222	arg1	macromolecules					224:237	biological macromolecules	213:237	biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability	213:374	Since 1971, the Protein Data Bank (PDB) has served as the single global archive for experimentally determined 3D structures of biological macromolecules made freely available to the global community according to the FAIR principles of Findability-Accessibility-Interoperability-Reusability.
33978738	6	94	theme	standardized	1575:1586	arg1	monosaccharides					1588:1602	standardized monosaccharides	1575:1602	standardized monosaccharides	1575:1602	For the first time, carbohydrates in PDB structures are consistently represented as collections of standardized monosaccharides, which precisely describe oligosaccharide structures and enable improved carbohydrate visualization, structure validation, robust quantitative and qualitative analyses, search for dendritic structures and classification.
33978738	5	95	theme	carbohydrates	1366:1378	arg1	descriptors					1351:1361	linear descriptors	1344:1361	linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins	1344:1473	The PDB Exchange MacroMolecular Crystallographic Information File data dictionary now supports (i) standardized atom nomenclature that conforms to International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology (IUPAC-IUBMB) recommendations for carbohydrates, (ii) uniform representation of branched entities for oligosaccharides, (iii) commonly used linear descriptors of carbohydrates developed by the glycoscience community and (iv) annotation of glycosylation sites in proteins.
33357929	1	0	theme	single	315:320	arg1	chains					322:327	two single chains	311:327	two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain	311:394	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	4	1	theme	cancer	800:805	arg1	cells					807:811	MCF-7 and 4T1 cancer cells	786:811	cells	807:811	As a result, the cellular internalization of the LNT-FITC into MCF-7 and 4T1 cancer cells was further increased by the aptamer conjugated to poly(dA).
33357929	1	2	theme	triple	332:337	arg1	s-LNT					366:370	s-LNT	366:370	s-LNT	366:370	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	1	2	theme	triple	332:337	arg1	lentinan					356:363	triple helical β-glucan lentinan	332:363	triple helical β-glucan lentinan (s-LNT)	332:371	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	1	3	theme	tumor-targetability	222:240	arg1	internalization					255:269	the tumor-targetability and cellular internalization	218:269	the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain	218:394	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	2	4	dep	in	401:402	arg1	vitro					404:408	vitro	404:408	vitro	404:408	The in vitro results demonstrate that the cellular uptake of s-LNT/poly(dA) composites in MCF-7 cancer cells was enhanced effectively after attaching the aptamer.
33357929	3	5	dep	-labelled	608:616	arg1	isothiocyanate					587:600	as-prepared fluorescin isothiocyanate	564:600	as-prepared fluorescin isothiocyanate	564:600	The as-prepared fluorescin isothiocyanate (FITC)-labelled LNT (LNT-FITC) through grafting was used for tracing the enhanced tumor-targetability of the composites.
33357929	0	6	theme	aptamer	102:108	arg1	conjugation					87:97	conjugation	87:97	conjugation of aptamer	87:108	Fabrication of tumor-targeting composites based on the triple helical β-glucan through conjugation of aptamer.
33357929	6	7	theme	delivery	1114:1121	arg1	β-glucan					1102:1109	triplex β-glucan	1094:1109	triplex β-glucan	1094:1109	This work provides a novel strategy for fabricating triplex β-glucan as delivery vectors with active tumor-targetability.
33357929	6	7	theme	delivery	1114:1121	arg1	vectors					1123:1129	delivery vectors	1114:1129	delivery vectors with active tumor-targetability	1114:1161	This work provides a novel strategy for fabricating triplex β-glucan as delivery vectors with active tumor-targetability.
33357929	1	8	theme	chain	390:394	arg1	chains					322:327	two single chains	311:327	two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain	311:394	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	6	9	theme	triplex	1094:1100	arg1	β-glucan					1102:1109	triplex β-glucan	1094:1109	triplex β-glucan	1094:1109	This work provides a novel strategy for fabricating triplex β-glucan as delivery vectors with active tumor-targetability.
33357929	6	9	theme	triplex	1094:1100	arg1	vectors					1123:1129	delivery vectors	1114:1129	delivery vectors with active tumor-targetability	1114:1161	This work provides a novel strategy for fabricating triplex β-glucan as delivery vectors with active tumor-targetability.
33357929	1	10	theme	cellular	246:253	arg1	internalization					255:269	the tumor-targetability and cellular internalization	218:269	the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain	218:394	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	1	11	attach	attached	149:156	arg1	tail					199:202	the polydeoxyadenylic acid (poly(dA)) tail	161:202	the polydeoxyadenylic acid (poly(dA)) tail	161:202	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	1	11	attach	attached	149:156	arg2	aptamers					135:142	the nucleic acid aptamers	118:142	the nucleic acid aptamers	118:142	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	2	12	theme	s-LNT/poly	458:467	arg1	composites					473:482	s-LNT/poly(dA) composites	458:482	s-LNT/poly(dA) composites	458:482	The in vitro results demonstrate that the cellular uptake of s-LNT/poly(dA) composites in MCF-7 cancer cells was enhanced effectively after attaching the aptamer.
33357929	2	12	theme	s-LNT/poly	458:467	arg1	dA					469:470	dA	469:470	dA	469:470	The in vitro results demonstrate that the cellular uptake of s-LNT/poly(dA) composites in MCF-7 cancer cells was enhanced effectively after attaching the aptamer.
33357929	1	13	theme	helical	339:345	arg1	s-LNT					366:370	s-LNT	366:370	s-LNT	366:370	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	1	13	theme	helical	339:345	arg1	lentinan					356:363	triple helical β-glucan lentinan	332:363	triple helical β-glucan lentinan (s-LNT)	332:371	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	5	14	dep	in	889:890	arg1	vivo					892:895	vivo	892:895	vivo	892:895	Meanwhile, the in vivo experiments further demonstrate more s-LNT/poly(dA)-aptamer composites were effectively accumulated at the tumor site compared with s-LNT alone.
33357929	1	15	theme	polydeoxyadenylic	165:181	arg1	tail					199:202	the polydeoxyadenylic acid (poly(dA)) tail	161:202	the polydeoxyadenylic acid (poly(dA)) tail	161:202	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	1	16	theme	β-glucan	347:354	arg1	s-LNT					366:370	s-LNT	366:370	s-LNT	366:370	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	1	16	theme	β-glucan	347:354	arg1	lentinan					356:363	triple helical β-glucan lentinan	332:363	triple helical β-glucan lentinan (s-LNT)	332:371	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	3	17	used	used	654:657	arg2	LNT-FITC					623:630	The as-prepared fluorescin isothiocyanate (FITC)-labelled LNT (LNT-FITC)	560:631	The as-prepared fluorescin isothiocyanate (FITC)-labelled LNT (LNT-FITC) through grafting	560:648	The as-prepared fluorescin isothiocyanate (FITC)-labelled LNT (LNT-FITC) through grafting was used for tracing the enhanced tumor-targetability of the composites.
33357929	5	18	theme	in	889:890	arg1	experiments					897:907	the in vivo experiments	885:907	the in vivo experiments	885:907	Meanwhile, the in vivo experiments further demonstrate more s-LNT/poly(dA)-aptamer composites were effectively accumulated at the tumor site compared with s-LNT alone.
33357929	1	19	theme	acid	183:186	arg1	tail					199:202	the polydeoxyadenylic acid (poly(dA)) tail	161:202	the polydeoxyadenylic acid (poly(dA)) tail	161:202	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	1	20	theme	lentinan	356:363	arg1	chains					322:327	two single chains	311:327	two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain	311:394	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	6	21	theme	novel	1063:1067	arg1	strategy					1069:1076	a novel strategy	1061:1076	a novel strategy for fabricating triplex β-glucan as delivery vectors with active tumor-targetability	1061:1161	This work provides a novel strategy for fabricating triplex β-glucan as delivery vectors with active tumor-targetability.
33357929	4	22	theme	MCF-7	786:790	arg1	cells					807:811	MCF-7 and 4T1 cancer cells	786:811	cells	807:811	As a result, the cellular internalization of the LNT-FITC into MCF-7 and 4T1 cancer cells was further increased by the aptamer conjugated to poly(dA).
33357929	1	23	theme	poly	189:192	arg1	tail					199:202	the polydeoxyadenylic acid (poly(dA)) tail	161:202	the polydeoxyadenylic acid (poly(dA)) tail	161:202	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	0	24	theme	composites	31:40	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of tumor-targeting composites	0:40	Fabrication of tumor-targeting composites based on the triple helical β-glucan through conjugation of aptamer.
33357929	1	25	theme	s-LNT/poly	274:283	arg1	composite					289:297	s-LNT/poly(dA) composite	274:297	s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain	274:394	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	1	25	theme	s-LNT/poly	274:283	arg1	dA					285:286	dA	285:286	dA	285:286	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	2	26	theme	MCF-7	487:491	arg1	cells					500:504	MCF-7 cancer cells	487:504	MCF-7 cancer cells	487:504	The in vitro results demonstrate that the cellular uptake of s-LNT/poly(dA) composites in MCF-7 cancer cells was enhanced effectively after attaching the aptamer.
33357929	4	27	theme	4T1	796:798	arg1	cells					807:811	MCF-7 and 4T1 cancer cells	786:811	cells	807:811	As a result, the cellular internalization of the LNT-FITC into MCF-7 and 4T1 cancer cells was further increased by the aptamer conjugated to poly(dA).
33357929	0	28	theme	tumor-targeting	15:29	arg1	composites					31:40	tumor-targeting composites	15:40	tumor-targeting composites	15:40	Fabrication of tumor-targeting composites based on the triple helical β-glucan through conjugation of aptamer.
33357929	3	29	theme	fluorescin	576:585	arg1	isothiocyanate					587:600	as-prepared fluorescin isothiocyanate	564:600	as-prepared fluorescin isothiocyanate	564:600	The as-prepared fluorescin isothiocyanate (FITC)-labelled LNT (LNT-FITC) through grafting was used for tracing the enhanced tumor-targetability of the composites.
33357929	3	30	theme	enhanced	675:682	arg1	tumor-targetability					684:702	the enhanced tumor-targetability	671:702	the enhanced tumor-targetability of the composites	671:720	The as-prepared fluorescin isothiocyanate (FITC)-labelled LNT (LNT-FITC) through grafting was used for tracing the enhanced tumor-targetability of the composites.
33357929	2	31	theme	composites	473:482	arg1	uptake					448:453	the cellular uptake	435:453	the cellular uptake of s-LNT/poly(dA) composites in MCF-7 cancer cells	435:504	The in vitro results demonstrate that the cellular uptake of s-LNT/poly(dA) composites in MCF-7 cancer cells was enhanced effectively after attaching the aptamer.
33357929	5	32	theme	-aptamer	948:955	arg1	composites					957:966	more s-LNT/poly(dA)-aptamer composites	929:966	more s-LNT/poly(dA)-aptamer composites	929:966	Meanwhile, the in vivo experiments further demonstrate more s-LNT/poly(dA)-aptamer composites were effectively accumulated at the tumor site compared with s-LNT alone.
33357929	5	33	dep	s-LNT/poly	934:943	arg1	dA					945:946	dA	945:946	dA	945:946	Meanwhile, the in vivo experiments further demonstrate more s-LNT/poly(dA)-aptamer composites were effectively accumulated at the tumor site compared with s-LNT alone.
33357929	2	34	from	uptake	448:453	arg1	cells					500:504	MCF-7 cancer cells	487:504	MCF-7 cancer cells	487:504	The in vitro results demonstrate that the cellular uptake of s-LNT/poly(dA) composites in MCF-7 cancer cells was enhanced effectively after attaching the aptamer.
33357929	4	35	theme	cellular	740:747	arg1	internalization					749:763	the cellular internalization	736:763	the cellular internalization of the LNT-FITC into MCF-7 and 4T1 cancer cells	736:811	As a result, the cellular internalization of the LNT-FITC into MCF-7 and 4T1 cancer cells was further increased by the aptamer conjugated to poly(dA).
33357929	4	35	theme	cellular	740:747	arg1	result					728:733	a result	726:733	a result	726:733	As a result, the cellular internalization of the LNT-FITC into MCF-7 and 4T1 cancer cells was further increased by the aptamer conjugated to poly(dA).
33357929	0	36	theme	triple	55:60	arg1	β-glucan					70:77	the triple helical β-glucan	51:77	the triple helical β-glucan through conjugation of aptamer	51:108	Fabrication of tumor-targeting composites based on the triple helical β-glucan through conjugation of aptamer.
33357929	1	37	theme	composite	289:297	arg1	internalization					255:269	the tumor-targetability and cellular internalization	218:269	the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain	218:394	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	5	38	theme	tumor	1004:1008	arg1	site					1010:1013	the tumor site	1000:1013	the tumor site	1000:1013	Meanwhile, the in vivo experiments further demonstrate more s-LNT/poly(dA)-aptamer composites were effectively accumulated at the tumor site compared with s-LNT alone.
33357929	2	39	theme	cellular	439:446	arg1	uptake					448:453	the cellular uptake	435:453	the cellular uptake of s-LNT/poly(dA) composites in MCF-7 cancer cells	435:504	The in vitro results demonstrate that the cellular uptake of s-LNT/poly(dA) composites in MCF-7 cancer cells was enhanced effectively after attaching the aptamer.
33357929	6	40	theme	active	1136:1141	arg1	tumor-targetability					1143:1161	active tumor-targetability	1136:1161	active tumor-targetability	1136:1161	This work provides a novel strategy for fabricating triplex β-glucan as delivery vectors with active tumor-targetability.
33357929	2	41	theme	in	401:402	arg1	results					410:416	The in vitro results	397:416	The in vitro results	397:416	The in vitro results demonstrate that the cellular uptake of s-LNT/poly(dA) composites in MCF-7 cancer cells was enhanced effectively after attaching the aptamer.
33357929	3	42	theme	LNT	618:620	arg1	LNT-FITC					623:630	The as-prepared fluorescin isothiocyanate (FITC)-labelled LNT (LNT-FITC)	560:631	The as-prepared fluorescin isothiocyanate (FITC)-labelled LNT (LNT-FITC) through grafting	560:648	The as-prepared fluorescin isothiocyanate (FITC)-labelled LNT (LNT-FITC) through grafting was used for tracing the enhanced tumor-targetability of the composites.
33357929	4	43	theme	LNT-FITC	772:779	arg1	internalization					749:763	the cellular internalization	736:763	the cellular internalization of the LNT-FITC into MCF-7 and 4T1 cancer cells	736:811	As a result, the cellular internalization of the LNT-FITC into MCF-7 and 4T1 cancer cells was further increased by the aptamer conjugated to poly(dA).
33357929	4	43	theme	LNT-FITC	772:779	arg1	result					728:733	a result	726:733	a result	726:733	As a result, the cellular internalization of the LNT-FITC into MCF-7 and 4T1 cancer cells was further increased by the aptamer conjugated to poly(dA).
33357929	1	44	theme	nucleic	122:128	arg1	aptamers					135:142	the nucleic acid aptamers	118:142	the nucleic acid aptamers	118:142	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	3	45	theme	composites	711:720	arg1	tumor-targetability					684:702	the enhanced tumor-targetability	671:702	the enhanced tumor-targetability of the composites	671:720	The as-prepared fluorescin isothiocyanate (FITC)-labelled LNT (LNT-FITC) through grafting was used for tracing the enhanced tumor-targetability of the composites.
33357929	1	46	theme	poly	381:384	arg1	chain					390:394	one poly(dA) chain	377:394	one poly(dA) chain	377:394	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	5	47	theme	s-LNT/poly	934:943	arg1	composites					957:966	more s-LNT/poly(dA)-aptamer composites	929:966	more s-LNT/poly(dA)-aptamer composites	929:966	Meanwhile, the in vivo experiments further demonstrate more s-LNT/poly(dA)-aptamer composites were effectively accumulated at the tumor site compared with s-LNT alone.
33357929	1	48	theme	acid	130:133	arg1	aptamers					135:142	the nucleic acid aptamers	118:142	the nucleic acid aptamers	118:142	Herein the nucleic acid aptamers were attached to the polydeoxyadenylic acid (poly(dA)) tail for improving the tumor-targetability and cellular internalization of s-LNT/poly(dA) composite composed of two single chains of triple helical β-glucan lentinan (s-LNT) and one poly(dA) chain.
33357929	2	49	theme	cancer	493:498	arg1	cells					500:504	MCF-7 cancer cells	487:504	MCF-7 cancer cells	487:504	The in vitro results demonstrate that the cellular uptake of s-LNT/poly(dA) composites in MCF-7 cancer cells was enhanced effectively after attaching the aptamer.
33357929	3	50	theme	as-prepared	564:574	arg1	isothiocyanate					587:600	as-prepared fluorescin isothiocyanate	564:600	as-prepared fluorescin isothiocyanate	564:600	The as-prepared fluorescin isothiocyanate (FITC)-labelled LNT (LNT-FITC) through grafting was used for tracing the enhanced tumor-targetability of the composites.
33357929	5	51	dep	Meanwhile	874:882	arg1	demonstrate					917:927	demonstrate	917:927	demonstrate more s-LNT/poly(dA)-aptamer composites	917:966	Meanwhile, the in vivo experiments further demonstrate more s-LNT/poly(dA)-aptamer composites were effectively accumulated at the tumor site compared with s-LNT alone.
33357929	3	52	theme	-labelled	608:616	arg1	LNT-FITC					623:630	The as-prepared fluorescin isothiocyanate (FITC)-labelled LNT (LNT-FITC)	560:631	The as-prepared fluorescin isothiocyanate (FITC)-labelled LNT (LNT-FITC) through grafting	560:648	The as-prepared fluorescin isothiocyanate (FITC)-labelled LNT (LNT-FITC) through grafting was used for tracing the enhanced tumor-targetability of the composites.
33357929	6	53	with	vectors	1123:1129	arg1	tumor-targetability					1143:1161	active tumor-targetability	1136:1161	active tumor-targetability	1136:1161	This work provides a novel strategy for fabricating triplex β-glucan as delivery vectors with active tumor-targetability.
33357929	0	54	theme	helical	62:68	arg1	β-glucan					70:77	the triple helical β-glucan	51:77	the triple helical β-glucan through conjugation of aptamer	51:108	Fabrication of tumor-targeting composites based on the triple helical β-glucan through conjugation of aptamer.
34702445	4	0	theme	Structure-activity	492:509	arg1	relationships					511:523	Structure-activity relationships	492:523	Structure-activity relationships	492:523	Structure-activity relationships evaluate UV dose and hydration state with respect to adhesive strength on soft tissue mimics.
34702445	5	1	theme	shear	757:761	arg1	forces					763:768	sustained shear forces	747:768	sustained shear forces	747:768	The bioadhesive composite has an adhesion strength ranging from 7 to 17 kPa and duration exceeding 48 h in wet conditions under sustained shear forces, while other mucoadhesives based on hydrophilic macromolecules exhibit adhesion strength of 0.5-5 kPa and last only a few hours.
34702445	5	2	theme	other	777:781	arg1	mucoadhesives					783:795	other mucoadhesives	777:795	other mucoadhesives based on hydrophilic macromolecules	777:831	The bioadhesive composite has an adhesion strength ranging from 7 to 17 kPa and duration exceeding 48 h in wet conditions under sustained shear forces, while other mucoadhesives based on hydrophilic macromolecules exhibit adhesion strength of 0.5-5 kPa and last only a few hours.
34702445	4	3	theme	UV	534:535	arg1	dose					537:540	UV dose	534:540	UV dose	534:540	Structure-activity relationships evaluate UV dose and hydration state with respect to adhesive strength on soft tissue mimics.
34702445	3	4	theme	carbene-based	355:367	arg1	PDz					383:385	PDz	383:385	PDz	383:385	Bacterial cellulose (BC) and photoactivated carbene-based bioadhesives (PDz) are combined to yield flexible film platform for interfacing soft tissues in dynamic, wet environments.
34702445	3	4	theme	carbene-based	355:367	arg1	bioadhesives					369:380	photoactivated carbene-based bioadhesives	340:380	photoactivated carbene-based bioadhesives (PDz)	340:386	Bacterial cellulose (BC) and photoactivated carbene-based bioadhesives (PDz) are combined to yield flexible film platform for interfacing soft tissues in dynamic, wet environments.
34702445	6	5	theme	BC	943:944	arg1	composites					946:955	BC composites	943:955	BC composites	943:955	The work highlights the first evaluation of BC composites for mucoadhesive treatments in the buccal cavity.
34702445	2	6	theme	oral	299:302	arg1	wound					304:308	oral wound	299:308	oral wound	299:308	A mucoadhesive drug delivery platform with extended soft tissue adhesion capability of up to 7 days is proposed for on-site management of oral wound.
34702445	5	7	contain	has	645:647	arg1	composite					635:643	The bioadhesive composite	619:643	The bioadhesive composite	619:643	The bioadhesive composite has an adhesion strength ranging from 7 to 17 kPa and duration exceeding 48 h in wet conditions under sustained shear forces, while other mucoadhesives based on hydrophilic macromolecules exhibit adhesion strength of 0.5-5 kPa and last only a few hours.
34702445	5	7	contain	has	645:647	arg2	strength					661:668	an adhesion strength	649:668	an adhesion strength ranging from 7 to 17 kPa and duration exceeding 48 h in wet conditions	649:739	The bioadhesive composite has an adhesion strength ranging from 7 to 17 kPa and duration exceeding 48 h in wet conditions under sustained shear forces, while other mucoadhesives based on hydrophilic macromolecules exhibit adhesion strength of 0.5-5 kPa and last only a few hours.
34702445	1	8	theme	Topical	70:76	arg1	approaches					78:87	Topical approaches	70:87	Topical approaches to oral diseases	70:104	Topical approaches to oral diseases require frequent dosing due to limited retention time.
34702445	4	9	theme	soft	599:602	arg1	tissue					604:609	soft tissue mimics	599:616	soft tissue mimics	599:616	Structure-activity relationships evaluate UV dose and hydration state with respect to adhesive strength on soft tissue mimics.
34702445	5	10	theme	sustained	747:755	arg1	forces					763:768	sustained shear forces	747:768	sustained shear forces	747:768	The bioadhesive composite has an adhesion strength ranging from 7 to 17 kPa and duration exceeding 48 h in wet conditions under sustained shear forces, while other mucoadhesives based on hydrophilic macromolecules exhibit adhesion strength of 0.5-5 kPa and last only a few hours.
34702445	5	11	theme	0.5-5 kPa	862:870	arg1	hours					892:896	last only a few hours	876:896	last only a few hours	876:896	The bioadhesive composite has an adhesion strength ranging from 7 to 17 kPa and duration exceeding 48 h in wet conditions under sustained shear forces, while other mucoadhesives based on hydrophilic macromolecules exhibit adhesion strength of 0.5-5 kPa and last only a few hours.
34702445	5	11	theme	0.5-5 kPa	862:870	arg1	strength					850:857	adhesion strength	841:857	adhesion strength of 0.5-5 kPa	841:870	The bioadhesive composite has an adhesion strength ranging from 7 to 17 kPa and duration exceeding 48 h in wet conditions under sustained shear forces, while other mucoadhesives based on hydrophilic macromolecules exhibit adhesion strength of 0.5-5 kPa and last only a few hours.
34702445	2	12	theme	on-site	277:283	arg1	management					285:294	on-site management	277:294	on-site management of oral wound	277:308	A mucoadhesive drug delivery platform with extended soft tissue adhesion capability of up to 7 days is proposed for on-site management of oral wound.
34702445	5	13	theme	few	888:890	arg1	hours					892:896	last only a few hours	876:896	last only a few hours	876:896	The bioadhesive composite has an adhesion strength ranging from 7 to 17 kPa and duration exceeding 48 h in wet conditions under sustained shear forces, while other mucoadhesives based on hydrophilic macromolecules exhibit adhesion strength of 0.5-5 kPa and last only a few hours.
34702445	3	14	theme	photoactivated	340:353	arg1	PDz					383:385	PDz	383:385	PDz	383:385	Bacterial cellulose (BC) and photoactivated carbene-based bioadhesives (PDz) are combined to yield flexible film platform for interfacing soft tissues in dynamic, wet environments.
34702445	3	14	theme	photoactivated	340:353	arg1	bioadhesives					369:380	photoactivated carbene-based bioadhesives	340:380	photoactivated carbene-based bioadhesives (PDz)	340:386	Bacterial cellulose (BC) and photoactivated carbene-based bioadhesives (PDz) are combined to yield flexible film platform for interfacing soft tissues in dynamic, wet environments.
34702445	0	15	theme	cellulose	10:18	arg1	composites					29:38	Bacterial cellulose adhesive composites	0:38	Bacterial cellulose adhesive composites for oral cavity applications	0:67	Bacterial cellulose adhesive composites for oral cavity applications.
34702445	1	16	theme	oral	92:95	arg1	diseases					97:104	oral diseases	92:104	oral diseases	92:104	Topical approaches to oral diseases require frequent dosing due to limited retention time.
34702445	3	17	theme	film	419:422	arg1	platform					424:431	flexible film platform	410:431	flexible film platform for interfacing soft tissues in dynamic, wet environments	410:489	Bacterial cellulose (BC) and photoactivated carbene-based bioadhesives (PDz) are combined to yield flexible film platform for interfacing soft tissues in dynamic, wet environments.
34702445	0	18	theme	Bacterial	0:8	arg1	composites					29:38	Bacterial cellulose adhesive composites	0:38	Bacterial cellulose adhesive composites for oral cavity applications	0:67	Bacterial cellulose adhesive composites for oral cavity applications.
34702445	6	19	theme	buccal	992:997	arg1	cavity					999:1004	the buccal cavity	988:1004	the buccal cavity	988:1004	The work highlights the first evaluation of BC composites for mucoadhesive treatments in the buccal cavity.
34702445	5	20	theme	adhesion	652:659	arg1	strength					661:668	an adhesion strength	649:668	an adhesion strength ranging from 7 to 17 kPa and duration exceeding 48 h in wet conditions	649:739	The bioadhesive composite has an adhesion strength ranging from 7 to 17 kPa and duration exceeding 48 h in wet conditions under sustained shear forces, while other mucoadhesives based on hydrophilic macromolecules exhibit adhesion strength of 0.5-5 kPa and last only a few hours.
34702445	5	21	theme	adhesion	841:848	arg1	strength					850:857	adhesion strength	841:857	adhesion strength of 0.5-5 kPa	841:870	The bioadhesive composite has an adhesion strength ranging from 7 to 17 kPa and duration exceeding 48 h in wet conditions under sustained shear forces, while other mucoadhesives based on hydrophilic macromolecules exhibit adhesion strength of 0.5-5 kPa and last only a few hours.
34702445	5	22	theme	bioadhesive	623:633	arg1	composite					635:643	The bioadhesive composite	619:643	The bioadhesive composite	619:643	The bioadhesive composite has an adhesion strength ranging from 7 to 17 kPa and duration exceeding 48 h in wet conditions under sustained shear forces, while other mucoadhesives based on hydrophilic macromolecules exhibit adhesion strength of 0.5-5 kPa and last only a few hours.
34702445	2	23	theme	wound	304:308	arg1	management					285:294	on-site management	277:294	on-site management of oral wound	277:308	A mucoadhesive drug delivery platform with extended soft tissue adhesion capability of up to 7 days is proposed for on-site management of oral wound.
34702445	0	24	theme	adhesive	20:27	arg1	composites					29:38	Bacterial cellulose adhesive composites	0:38	Bacterial cellulose adhesive composites for oral cavity applications	0:67	Bacterial cellulose adhesive composites for oral cavity applications.
34702445	4	25	theme	adhesive	578:585	arg1	strength					587:594	adhesive strength	578:594	adhesive strength on soft tissue mimics	578:616	Structure-activity relationships evaluate UV dose and hydration state with respect to adhesive strength on soft tissue mimics.
34702445	6	26	from	treatments	974:983	arg1	cavity					999:1004	the buccal cavity	988:1004	the buccal cavity	988:1004	The work highlights the first evaluation of BC composites for mucoadhesive treatments in the buccal cavity.
34702445	4	27	dep	tissue	604:609	arg1	mimics					611:616	mimics	611:616	mimics	611:616	Structure-activity relationships evaluate UV dose and hydration state with respect to adhesive strength on soft tissue mimics.
34702445	1	28	theme	frequent	114:121	arg1	dosing					123:128	frequent dosing	114:128	frequent dosing due to limited retention time	114:158	Topical approaches to oral diseases require frequent dosing due to limited retention time.
34702445	3	29	theme	dynamic	465:471	arg1	environments					478:489	dynamic, wet environments	465:489	dynamic, wet environments	465:489	Bacterial cellulose (BC) and photoactivated carbene-based bioadhesives (PDz) are combined to yield flexible film platform for interfacing soft tissues in dynamic, wet environments.
34702445	0	30	theme	oral	44:47	arg1	applications					56:67	oral cavity applications	44:67	oral cavity applications	44:67	Bacterial cellulose adhesive composites for oral cavity applications.
34702445	2	31	theme	delivery	181:188	arg1	platform					190:197	A mucoadhesive drug delivery platform	161:197	A mucoadhesive drug delivery platform with extended soft tissue adhesion capability of up to 7 days	161:259	A mucoadhesive drug delivery platform with extended soft tissue adhesion capability of up to 7 days is proposed for on-site management of oral wound.
34702445	2	32	theme	up	248:249	arg1	capability					234:243	extended soft tissue adhesion capability	204:243	extended soft tissue adhesion capability of up to 7 days	204:259	A mucoadhesive drug delivery platform with extended soft tissue adhesion capability of up to 7 days is proposed for on-site management of oral wound.
34702445	3	33	theme	soft	449:452	arg1	tissues					454:460	soft tissues	449:460	soft tissues	449:460	Bacterial cellulose (BC) and photoactivated carbene-based bioadhesives (PDz) are combined to yield flexible film platform for interfacing soft tissues in dynamic, wet environments.
34702445	3	34	theme	flexible	410:417	arg1	platform					424:431	flexible film platform	410:431	flexible film platform for interfacing soft tissues in dynamic, wet environments	410:489	Bacterial cellulose (BC) and photoactivated carbene-based bioadhesives (PDz) are combined to yield flexible film platform for interfacing soft tissues in dynamic, wet environments.
34702445	6	35	theme	mucoadhesive	961:972	arg1	treatments					974:983	mucoadhesive treatments	961:983	mucoadhesive treatments in the buccal cavity	961:1004	The work highlights the first evaluation of BC composites for mucoadhesive treatments in the buccal cavity.
34702445	5	36	theme	wet	726:728	arg1	conditions					730:739	wet conditions	726:739	wet conditions	726:739	The bioadhesive composite has an adhesion strength ranging from 7 to 17 kPa and duration exceeding 48 h in wet conditions under sustained shear forces, while other mucoadhesives based on hydrophilic macromolecules exhibit adhesion strength of 0.5-5 kPa and last only a few hours.
34702445	2	37	theme	drug	176:179	arg1	platform					190:197	A mucoadhesive drug delivery platform	161:197	A mucoadhesive drug delivery platform with extended soft tissue adhesion capability of up to 7 days	161:259	A mucoadhesive drug delivery platform with extended soft tissue adhesion capability of up to 7 days is proposed for on-site management of oral wound.
34702445	4	38	from	strength	587:594	arg1	tissue					604:609	soft tissue mimics	599:616	soft tissue mimics	599:616	Structure-activity relationships evaluate UV dose and hydration state with respect to adhesive strength on soft tissue mimics.
34702445	5	39	theme	hydrophilic	806:816	arg1	macromolecules					818:831	hydrophilic macromolecules	806:831	hydrophilic macromolecules	806:831	The bioadhesive composite has an adhesion strength ranging from 7 to 17 kPa and duration exceeding 48 h in wet conditions under sustained shear forces, while other mucoadhesives based on hydrophilic macromolecules exhibit adhesion strength of 0.5-5 kPa and last only a few hours.
34702445	2	40	theme	mucoadhesive	163:174	arg1	platform					190:197	A mucoadhesive drug delivery platform	161:197	A mucoadhesive drug delivery platform with extended soft tissue adhesion capability of up to 7 days	161:259	A mucoadhesive drug delivery platform with extended soft tissue adhesion capability of up to 7 days is proposed for on-site management of oral wound.
34702445	0	41	theme	cavity	49:54	arg1	applications					56:67	oral cavity applications	44:67	oral cavity applications	44:67	Bacterial cellulose adhesive composites for oral cavity applications.
34702445	6	42	theme	composites	946:955	arg1	evaluation					929:938	the first evaluation	919:938	the first evaluation of BC composites for mucoadhesive treatments in the buccal cavity	919:1004	The work highlights the first evaluation of BC composites for mucoadhesive treatments in the buccal cavity.
34702445	3	43	theme	Bacterial	311:319	arg1	BC					332:333	BC	332:333	BC	332:333	Bacterial cellulose (BC) and photoactivated carbene-based bioadhesives (PDz) are combined to yield flexible film platform for interfacing soft tissues in dynamic, wet environments.
34702445	3	43	theme	Bacterial	311:319	arg1	cellulose					321:329	Bacterial cellulose	311:329	Bacterial cellulose (BC)	311:334	Bacterial cellulose (BC) and photoactivated carbene-based bioadhesives (PDz) are combined to yield flexible film platform for interfacing soft tissues in dynamic, wet environments.
34702445	2	44	theme	tissue	218:223	arg1	capability					234:243	extended soft tissue adhesion capability	204:243	extended soft tissue adhesion capability of up to 7 days	204:259	A mucoadhesive drug delivery platform with extended soft tissue adhesion capability of up to 7 days is proposed for on-site management of oral wound.
34702445	2	45	theme	adhesion	225:232	arg1	capability					234:243	extended soft tissue adhesion capability	204:243	extended soft tissue adhesion capability of up to 7 days	204:259	A mucoadhesive drug delivery platform with extended soft tissue adhesion capability of up to 7 days is proposed for on-site management of oral wound.
34702445	1	46	theme	due	130:132	arg1	dosing					123:128	frequent dosing	114:128	frequent dosing due to limited retention time	114:158	Topical approaches to oral diseases require frequent dosing due to limited retention time.
34702445	4	47	theme	hydration	546:554	arg1	state					556:560	hydration state	546:560	hydration state	546:560	Structure-activity relationships evaluate UV dose and hydration state with respect to adhesive strength on soft tissue mimics.
34702445	2	48	theme	soft	213:216	arg1	capability					234:243	extended soft tissue adhesion capability	204:243	extended soft tissue adhesion capability of up to 7 days	204:259	A mucoadhesive drug delivery platform with extended soft tissue adhesion capability of up to 7 days is proposed for on-site management of oral wound.
34702445	2	49	with	platform	190:197	arg1	capability					234:243	extended soft tissue adhesion capability	204:243	extended soft tissue adhesion capability of up to 7 days	204:259	A mucoadhesive drug delivery platform with extended soft tissue adhesion capability of up to 7 days is proposed for on-site management of oral wound.
34702445	6	50	theme	first	923:927	arg1	evaluation					929:938	the first evaluation	919:938	the first evaluation of BC composites for mucoadhesive treatments in the buccal cavity	919:1004	The work highlights the first evaluation of BC composites for mucoadhesive treatments in the buccal cavity.
34702445	2	51	theme	extended	204:211	arg1	capability					234:243	extended soft tissue adhesion capability	204:243	extended soft tissue adhesion capability of up to 7 days	204:259	A mucoadhesive drug delivery platform with extended soft tissue adhesion capability of up to 7 days is proposed for on-site management of oral wound.
34702445	1	52	theme	limited	137:143	arg1	time					155:158	limited retention time	137:158	limited retention time	137:158	Topical approaches to oral diseases require frequent dosing due to limited retention time.
34702445	5	53	theme	last	876:879	arg1	hours					892:896	last only a few hours	876:896	last only a few hours	876:896	The bioadhesive composite has an adhesion strength ranging from 7 to 17 kPa and duration exceeding 48 h in wet conditions under sustained shear forces, while other mucoadhesives based on hydrophilic macromolecules exhibit adhesion strength of 0.5-5 kPa and last only a few hours.
34702445	1	54	theme	retention	145:153	arg1	time					155:158	limited retention time	137:158	limited retention time	137:158	Topical approaches to oral diseases require frequent dosing due to limited retention time.
34702445	3	55	dep	dynamic	465:471	arg1	wet					474:476	wet	474:476	wet	474:476	Bacterial cellulose (BC) and photoactivated carbene-based bioadhesives (PDz) are combined to yield flexible film platform for interfacing soft tissues in dynamic, wet environments.
32935963	6	0	theme	±	1147:1147	arg1	GPa					1153:1155	1.5 ± 0.2 to 2.7 ± 0.4 GPa	1130:1155	1.5 ± 0.2 to 2.7 ± 0.4 GPa	1130:1155	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	8	1	theme	current	1606:1612	arg1	literature					1614:1623	the current literature	1602:1623	the current literature on all-cellulose composites	1602:1651	This study adds to the current literature on all-cellulose composites and helps pave the route for the technical and economical feasibilities of replacing non-renewable, non-biodegradable plastics in short-term applications by materials that are both renewable and biodegradable, that are also produced through green protocols and isolated from surplus biomass, and that still perform similarly or even better.
32935963	7	2	theme	high-energy	1538:1548	arg1	mixing					1550:1555	high-energy mixing	1538:1555	high-energy mixing	1538:1555	This dual, win-win effect of BCNCs on the mechanical and barrier properties of HPMC films was enabled by a suitable dispersion state, achieved via high-energy mixing, and quenched by casting.
32935963	8	3	from	literature	1614:1623	arg1	composites					1642:1651	all-cellulose composites	1628:1651	all-cellulose composites	1628:1651	This study adds to the current literature on all-cellulose composites and helps pave the route for the technical and economical feasibilities of replacing non-renewable, non-biodegradable plastics in short-term applications by materials that are both renewable and biodegradable, that are also produced through green protocols and isolated from surplus biomass, and that still perform similarly or even better.
32935963	6	4	theme	±	1134:1134	arg1	GPa					1153:1155	1.5 ± 0.2 to 2.7 ± 0.4 GPa	1130:1155	1.5 ± 0.2 to 2.7 ± 0.4 GPa	1130:1155	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	7	5	from	effect	1410:1415	arg1	properties					1456:1465	the mechanical and barrier properties	1429:1465	properties	1456:1465	This dual, win-win effect of BCNCs on the mechanical and barrier properties of HPMC films was enabled by a suitable dispersion state, achieved via high-energy mixing, and quenched by casting.
32935963	6	6	theme	nm	1342:1343	arg1	±					1336:1336	120 ± 31	1332:1339	120 ± 31	1332:1339	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	6	theme	nm	1342:1343	arg1	nm					1342:1343	(120 ± 31) nm	1331:1343	(120 ± 31) nm long	1331:1348	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	6	theme	nm	1342:1343	arg1	%					1326:1326	only 5 wt %	1316:1326	only 5 wt % of (120 ± 31) nm long	1316:1348	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	7	theme	transparent	985:995	arg1	HPMC					1028:1031	HPMC	1028:1031	HPMC	1028:1031	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	7	theme	transparent	985:995	arg1	methylcellulose					1011:1025	the optically transparent hydroxypropyl methylcellulose	971:1025	the optically transparent hydroxypropyl methylcellulose (HPMC) films	971:1038	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	8	8	theme	surplus	1928:1934	arg1	biomass					1936:1942	surplus biomass	1928:1942	surplus biomass	1928:1942	This study adds to the current literature on all-cellulose composites and helps pave the route for the technical and economical feasibilities of replacing non-renewable, non-biodegradable plastics in short-term applications by materials that are both renewable and biodegradable, that are also produced through green protocols and isolated from surplus biomass, and that still perform similarly or even better.
32935963	0	9	from	Fillers	86:92	arg1	Composites					111:120	All-Cellulose Composites	97:120	All-Cellulose Composites	97:120	Upcycling Microbial Cellulose Scraps into Nanowhiskers with Engineered Performance as Fillers in All-Cellulose Composites.
32935963	4	10	theme	unsustainable	665:677	arg1	plastics					695:702	unsustainable, fossil-derived plastics	665:702	unsustainable, fossil-derived plastics intended for single-use applications	665:739	Optimum performance as a packaging material was achieved by engineering BCNC surface chemistry as well as the filler-in-matrix dispersion, targeting the replacement of unsustainable, fossil-derived plastics intended for single-use applications.
32935963	6	11	theme	water	1221:1225	arg1	permeability					1233:1244	water vapor permeability	1221:1244	water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1)	1221:1297	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	11	theme	water	1221:1225	arg1	kPa-1					1292:1296	from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1	1247:1296	from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1	1247:1296	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	12	theme	%	1371:1371	arg1	nm					1360:1361	reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide	1164:1366	reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide	1164:1366	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	12	theme	%	1371:1371	arg1	BCNC					1385:1388	88% crystalline BCNC	1369:1388	88% crystalline BCNC	1369:1388	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	13	theme	elongation	1172:1181	arg1	nm					1360:1361	reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide	1164:1366	reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide	1164:1366	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	13	theme	elongation	1172:1181	arg1	BCNC					1385:1388	88% crystalline BCNC	1369:1388	88% crystalline BCNC	1369:1388	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	5	14	theme	biomass	939:945	arg1	use					932:934	the integral use	919:934	the integral use of biomass	919:945	Cost could pose a hurdle, eliminated through the valorization of underutilized scraps from industrial operations, which is also in line with the circular bioeconomy in terms of the integral use of biomass.
32935963	8	15	theme	technical	1686:1694	arg1	feasibilities					1711:1723	the technical and economical feasibilities	1682:1723	the technical and economical feasibilities of replacing non-renewable, non-biodegradable plastics in short-term applications by materials that are both renewable and biodegradable, that are also produced through green protocols and isolated from surplus biomass, and that still perform similarly or even better	1682:1991	This study adds to the current literature on all-cellulose composites and helps pave the route for the technical and economical feasibilities of replacing non-renewable, non-biodegradable plastics in short-term applications by materials that are both renewable and biodegradable, that are also produced through green protocols and isolated from surplus biomass, and that still perform similarly or even better.
32935963	6	16	theme	improved	1050:1057	arg1	strength					1067:1074	improved tensile strength	1050:1074	improved tensile strength (from 61 ± 6 to 86 ± 9 MPa)	1050:1102	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	3	17	theme	cellulose	340:348	arg1	architecture					324:335	the multiscale architecture	309:335	the multiscale architecture of cellulose	309:348	Inspired by the multiscale architecture of cellulose, we report on all-cellulose composites comprising cellulose ether as a matrix and highly pure bacterial cellulose nanocrystals (BCNCs) as fillers.
32935963	4	18	theme	single-use	717:726	arg1	applications					728:739	single-use applications	717:739	single-use applications	717:739	Optimum performance as a packaging material was achieved by engineering BCNC surface chemistry as well as the filler-in-matrix dispersion, targeting the replacement of unsustainable, fossil-derived plastics intended for single-use applications.
32935963	8	19	theme	economical	1700:1709	arg1	feasibilities					1711:1723	the technical and economical feasibilities	1682:1723	the technical and economical feasibilities of replacing non-renewable, non-biodegradable plastics in short-term applications by materials that are both renewable and biodegradable, that are also produced through green protocols and isolated from surplus biomass, and that still perform similarly or even better	1682:1991	This study adds to the current literature on all-cellulose composites and helps pave the route for the technical and economical feasibilities of replacing non-renewable, non-biodegradable plastics in short-term applications by materials that are both renewable and biodegradable, that are also produced through green protocols and isolated from surplus biomass, and that still perform similarly or even better.
32935963	7	20	dep	dual	1396:1399	arg1	win-win					1402:1408	win-win	1402:1408	win-win	1402:1408	This dual, win-win effect of BCNCs on the mechanical and barrier properties of HPMC films was enabled by a suitable dispersion state, achieved via high-energy mixing, and quenched by casting.
32935963	7	21	theme	films	1475:1479	arg1	properties					1456:1465	the mechanical and barrier properties	1429:1465	properties	1456:1465	This dual, win-win effect of BCNCs on the mechanical and barrier properties of HPMC films was enabled by a suitable dispersion state, achieved via high-energy mixing, and quenched by casting.
32935963	5	22	theme	underutilized	807:819	arg1	scraps					821:826	underutilized scraps	807:826	underutilized scraps from industrial operations	807:853	Cost could pose a hurdle, eliminated through the valorization of underutilized scraps from industrial operations, which is also in line with the circular bioeconomy in terms of the integral use of biomass.
32935963	5	23	theme	use	932:934	arg1	terms					910:914	terms	910:914	terms of the integral use of biomass	910:945	Cost could pose a hurdle, eliminated through the valorization of underutilized scraps from industrial operations, which is also in line with the circular bioeconomy in terms of the integral use of biomass.
32935963	5	24	theme	industrial	833:842	arg1	operations					844:853	industrial operations	833:853	industrial operations	833:853	Cost could pose a hurdle, eliminated through the valorization of underutilized scraps from industrial operations, which is also in line with the circular bioeconomy in terms of the integral use of biomass.
32935963	6	25	theme	±	1257:1257	arg1	±					1272:1272	±	1272:1272	±	1272:1272	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	7	26	theme	barrier	1448:1454	arg1	properties					1456:1465	the mechanical and barrier properties	1429:1465	properties	1456:1465	This dual, win-win effect of BCNCs on the mechanical and barrier properties of HPMC films was enabled by a suitable dispersion state, achieved via high-energy mixing, and quenched by casting.
32935963	6	27	theme	methylcellulose	1011:1025	arg1	films					1034:1038	the optically transparent hydroxypropyl methylcellulose (HPMC) films	971:1038	the optically transparent hydroxypropyl methylcellulose (HPMC) films	971:1038	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	3	28	theme	bacterial	444:452	arg1	ether					410:414	cellulose ether	400:414	cellulose ether	400:414	Inspired by the multiscale architecture of cellulose, we report on all-cellulose composites comprising cellulose ether as a matrix and highly pure bacterial cellulose nanocrystals (BCNCs) as fillers.
32935963	3	28	theme	bacterial	444:452	arg1	BCNCs					478:482	BCNCs	478:482	BCNCs	478:482	Inspired by the multiscale architecture of cellulose, we report on all-cellulose composites comprising cellulose ether as a matrix and highly pure bacterial cellulose nanocrystals (BCNCs) as fillers.
32935963	3	28	theme	bacterial	444:452	arg1	nanocrystals					464:475	bacterial cellulose nanocrystals	444:475	highly pure bacterial cellulose nanocrystals (BCNCs)	432:483	Inspired by the multiscale architecture of cellulose, we report on all-cellulose composites comprising cellulose ether as a matrix and highly pure bacterial cellulose nanocrystals (BCNCs) as fillers.
32935963	5	29	theme	scraps	821:826	arg1	valorization					791:802	the valorization	787:802	the valorization of underutilized scraps from industrial operations	787:853	Cost could pose a hurdle, eliminated through the valorization of underutilized scraps from industrial operations, which is also in line with the circular bioeconomy in terms of the integral use of biomass.
32935963	7	30	theme	mechanical	1433:1442	arg1	properties					1456:1465	the mechanical and barrier properties	1429:1465	properties	1456:1465	This dual, win-win effect of BCNCs on the mechanical and barrier properties of HPMC films was enabled by a suitable dispersion state, achieved via high-energy mixing, and quenched by casting.
32935963	3	31	theme	cellulose	400:408	arg1	ether					410:414	cellulose ether	400:414	cellulose ether	400:414	Inspired by the multiscale architecture of cellulose, we report on all-cellulose composites comprising cellulose ether as a matrix and highly pure bacterial cellulose nanocrystals (BCNCs) as fillers.
32935963	3	31	theme	cellulose	400:408	arg1	matrix					421:426	a matrix	419:426	a matrix	419:426	Inspired by the multiscale architecture of cellulose, we report on all-cellulose composites comprising cellulose ether as a matrix and highly pure bacterial cellulose nanocrystals (BCNCs) as fillers.
32935963	3	31	theme	cellulose	400:408	arg1	nanocrystals					464:475	bacterial cellulose nanocrystals	444:475	highly pure bacterial cellulose nanocrystals (BCNCs)	432:483	Inspired by the multiscale architecture of cellulose, we report on all-cellulose composites comprising cellulose ether as a matrix and highly pure bacterial cellulose nanocrystals (BCNCs) as fillers.
32935963	6	32	dep	±	1272:1272	arg1	to					1264:1265	to	1264:1265	to	1264:1265	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	8	33	theme	all-cellulose	1628:1640	arg1	composites					1642:1651	all-cellulose composites	1628:1651	all-cellulose composites	1628:1651	This study adds to the current literature on all-cellulose composites and helps pave the route for the technical and economical feasibilities of replacing non-renewable, non-biodegradable plastics in short-term applications by materials that are both renewable and biodegradable, that are also produced through green protocols and isolated from surplus biomass, and that still perform similarly or even better.
32935963	6	34	theme	±	1085:1085	arg1	MPa					1099:1101	61 ± 6 to 86 ± 9 MPa	1082:1101	61 ± 6 to 86 ± 9 MPa	1082:1101	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	35	dep	86	1092:1093	arg1	to					1089:1090	to	1089:1090	to	1089:1090	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	0	36	theme	Cellulose	20:28	arg1	Scraps					30:35	Microbial Cellulose Scraps	10:35	Microbial Cellulose Scraps	10:35	Upcycling Microbial Cellulose Scraps into Nanowhiskers with Engineered Performance as Fillers in All-Cellulose Composites.
32935963	4	37	theme	surface	574:580	arg1	chemistry					582:590	BCNC surface chemistry	569:590	BCNC surface chemistry as well as the filler-in-matrix dispersion, targeting the replacement of unsustainable, fossil-derived plastics intended for single-use applications	569:739	Optimum performance as a packaging material was achieved by engineering BCNC surface chemistry as well as the filler-in-matrix dispersion, targeting the replacement of unsustainable, fossil-derived plastics intended for single-use applications.
32935963	6	38	theme	h-1	1284:1286	arg1	permeability					1233:1244	water vapor permeability	1221:1244	water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1)	1221:1297	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	38	theme	h-1	1284:1286	arg1	kPa-1					1292:1296	from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1	1247:1296	from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1	1247:1296	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	39	from	%	1214:1214	arg1	permeability					1233:1244	water vapor permeability	1221:1244	water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1)	1221:1297	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	39	from	%	1214:1214	arg1	kPa-1					1292:1296	from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1	1247:1296	from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1	1247:1296	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	39	from	%	1214:1214	arg1	break					1186:1190	break	1186:1190	break (from 15 ± 2 to 12 ± 2%)	1186:1215	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	8	40	dep	renewable	1834:1842	arg1	perform					1960:1966	perform	1960:1966	perform similarly or even better	1960:1991	This study adds to the current literature on all-cellulose composites and helps pave the route for the technical and economical feasibilities of replacing non-renewable, non-biodegradable plastics in short-term applications by materials that are both renewable and biodegradable, that are also produced through green protocols and isolated from surplus biomass, and that still perform similarly or even better.
32935963	8	40	dep	renewable	1834:1842	arg1	isolated					1914:1921	isolated	1914:1921	isolated from surplus biomass	1914:1942	This study adds to the current literature on all-cellulose composites and helps pave the route for the technical and economical feasibilities of replacing non-renewable, non-biodegradable plastics in short-term applications by materials that are both renewable and biodegradable, that are also produced through green protocols and isolated from surplus biomass, and that still perform similarly or even better.
32935963	8	40	dep	renewable	1834:1842	arg1	produced					1877:1884	produced	1877:1884	produced through green protocols	1877:1908	This study adds to the current literature on all-cellulose composites and helps pave the route for the technical and economical feasibilities of replacing non-renewable, non-biodegradable plastics in short-term applications by materials that are both renewable and biodegradable, that are also produced through green protocols and isolated from surplus biomass, and that still perform similarly or even better.
32935963	6	41	theme	g	1279:1279	arg1	permeability					1233:1244	water vapor permeability	1221:1244	water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1)	1221:1297	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	41	theme	g	1279:1279	arg1	kPa-1					1292:1296	from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1	1247:1296	from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1	1247:1296	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	4	42	theme	filler-in-matrix	607:622	arg1	dispersion					624:633	the filler-in-matrix dispersion	603:633	the filler-in-matrix dispersion	603:633	Optimum performance as a packaging material was achieved by engineering BCNC surface chemistry as well as the filler-in-matrix dispersion, targeting the replacement of unsustainable, fossil-derived plastics intended for single-use applications.
32935963	6	43	theme	mm	1281:1282	arg1	permeability					1233:1244	water vapor permeability	1221:1244	water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1)	1221:1297	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	43	theme	mm	1281:1282	arg1	kPa-1					1292:1296	from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1	1247:1296	from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1	1247:1296	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	44	theme	±	1211:1211	arg1	%					1214:1214	15 ± 2 to 12 ± 2%	1198:1214	15 ± 2 to 12 ± 2%	1198:1214	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	45	from	MPa	1099:1101	arg1	strength					1067:1074	improved tensile strength	1050:1074	improved tensile strength (from 61 ± 6 to 86 ± 9 MPa)	1050:1102	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	45	from	MPa	1099:1101	arg1	modulus					1116:1122	Young's modulus	1108:1122	Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa)	1108:1156	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	46	theme	±	1095:1095	arg1	MPa					1099:1101	61 ± 6 to 86 ± 9 MPa	1082:1101	61 ± 6 to 86 ± 9 MPa	1082:1101	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	8	47	dep	non-renewable	1738:1750	arg1	non-biodegradable					1753:1769	non-biodegradable	1753:1769	non-biodegradable	1753:1769	This study adds to the current literature on all-cellulose composites and helps pave the route for the technical and economical feasibilities of replacing non-renewable, non-biodegradable plastics in short-term applications by materials that are both renewable and biodegradable, that are also produced through green protocols and isolated from surplus biomass, and that still perform similarly or even better.
32935963	6	48	theme	±	1201:1201	arg1	%					1214:1214	15 ± 2 to 12 ± 2%	1198:1214	15 ± 2 to 12 ± 2%	1198:1214	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	2	49	theme	carbon	284:289	arg1	sink					291:294	a carbon sink	282:294	a carbon sink	282:294	Unlike the latter, cellulose biodegrades and may represent a carbon sink.
32935963	5	50	theme	circular	887:894	arg1	bioeconomy					896:905	the circular bioeconomy	883:905	the circular bioeconomy in terms of the integral use of biomass	883:945	Cost could pose a hurdle, eliminated through the valorization of underutilized scraps from industrial operations, which is also in line with the circular bioeconomy in terms of the integral use of biomass.
32935963	6	51	dep	12	1208:1209	arg1	to					1205:1206	to	1205:1206	to	1205:1206	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	52	theme	long	1345:1348	arg1	±					1336:1336	120 ± 31	1332:1339	120 ± 31	1332:1339	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	52	theme	long	1345:1348	arg1	nm					1342:1343	(120 ± 31) nm	1331:1343	(120 ± 31) nm long	1331:1348	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	53	theme	hydroxypropyl	997:1009	arg1	HPMC					1028:1031	HPMC	1028:1031	HPMC	1028:1031	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	53	theme	hydroxypropyl	997:1009	arg1	methylcellulose					1011:1025	the optically transparent hydroxypropyl methylcellulose	971:1025	the optically transparent hydroxypropyl methylcellulose (HPMC) films	971:1038	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	7	54	theme	dispersion	1507:1516	arg1	state					1518:1522	a suitable dispersion state	1496:1522	a suitable dispersion state	1496:1522	This dual, win-win effect of BCNCs on the mechanical and barrier properties of HPMC films was enabled by a suitable dispersion state, achieved via high-energy mixing, and quenched by casting.
32935963	6	55	theme	1.5	1130:1132	arg1	±					1134:1134	±	1134:1134	±	1134:1134	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	4	56	theme	plastics	695:702	arg1	replacement					650:660	the replacement	646:660	the replacement of unsustainable, fossil-derived plastics intended for single-use applications	646:739	Optimum performance as a packaging material was achieved by engineering BCNC surface chemistry as well as the filler-in-matrix dispersion, targeting the replacement of unsustainable, fossil-derived plastics intended for single-use applications.
32935963	7	57	theme	dual	1396:1399	arg1	effect					1410:1415	This dual, win-win effect	1391:1415	This dual, win-win effect of BCNCs on the mechanical and barrier properties of HPMC films	1391:1479	This dual, win-win effect of BCNCs on the mechanical and barrier properties of HPMC films was enabled by a suitable dispersion state, achieved via high-energy mixing, and quenched by casting.
32935963	6	58	theme	vapor	1227:1231	arg1	permeability					1233:1244	water vapor permeability	1221:1244	water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1)	1221:1297	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	58	theme	vapor	1227:1231	arg1	kPa-1					1292:1296	from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1	1247:1296	from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1	1247:1296	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	59	dep	2.7	1143:1145	arg1	to					1140:1141	to	1140:1141	to	1140:1141	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	60	theme	88	1369:1370	arg1	%					1371:1371	%	1371:1371	%	1371:1371	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	3	61	theme	multiscale	313:322	arg1	architecture					324:335	the multiscale architecture	309:335	the multiscale architecture of cellulose	309:348	Inspired by the multiscale architecture of cellulose, we report on all-cellulose composites comprising cellulose ether as a matrix and highly pure bacterial cellulose nanocrystals (BCNCs) as fillers.
32935963	8	62	from	plastics	1771:1778	arg1	applications					1794:1805	short-term applications	1783:1805	short-term applications	1783:1805	This study adds to the current literature on all-cellulose composites and helps pave the route for the technical and economical feasibilities of replacing non-renewable, non-biodegradable plastics in short-term applications by materials that are both renewable and biodegradable, that are also produced through green protocols and isolated from surplus biomass, and that still perform similarly or even better.
32935963	6	63	theme	reduced	1164:1170	arg1	nm					1360:1361	reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide	1164:1366	reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide	1164:1366	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	63	theme	reduced	1164:1170	arg1	BCNC					1385:1388	88% crystalline BCNC	1369:1388	88% crystalline BCNC	1369:1388	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	64	theme	wide	1363:1366	arg1	nm					1360:1361	reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide	1164:1366	reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide	1164:1366	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	64	theme	wide	1363:1366	arg1	BCNC					1385:1388	88% crystalline BCNC	1369:1388	88% crystalline BCNC	1369:1388	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	7	65	theme	suitable	1498:1505	arg1	state					1518:1522	a suitable dispersion state	1496:1522	a suitable dispersion state	1496:1522	This dual, win-win effect of BCNCs on the mechanical and barrier properties of HPMC films was enabled by a suitable dispersion state, achieved via high-energy mixing, and quenched by casting.
32935963	6	66	theme	tensile	1059:1065	arg1	strength					1067:1074	improved tensile strength	1050:1074	improved tensile strength (from 61 ± 6 to 86 ± 9 MPa)	1050:1102	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	67	from	break	1186:1190	arg1	elongation					1172:1181	elongation	1172:1181	elongation	1172:1181	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	67	from	break	1186:1190	arg1	±					1355:1355	13 ± 3	1352:1357	13 ± 3	1352:1357	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	68	theme	0.01	1274:1277	arg1	±					1272:1272	±	1272:1272	±	1272:1272	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	5	69	from	valorization	791:802	arg1	operations					844:853	industrial operations	833:853	industrial operations	833:853	Cost could pose a hurdle, eliminated through the valorization of underutilized scraps from industrial operations, which is also in line with the circular bioeconomy in terms of the integral use of biomass.
32935963	6	70	from	GPa	1153:1155	arg1	strength					1067:1074	improved tensile strength	1050:1074	improved tensile strength (from 61 ± 6 to 86 ± 9 MPa)	1050:1102	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	70	from	GPa	1153:1155	arg1	modulus					1116:1122	Young's modulus	1108:1122	Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa)	1108:1156	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	0	71	theme	All-Cellulose	97:109	arg1	Composites					111:120	All-Cellulose Composites	97:120	All-Cellulose Composites	97:120	Upcycling Microbial Cellulose Scraps into Nanowhiskers with Engineered Performance as Fillers in All-Cellulose Composites.
32935963	6	72	theme	0.31	1267:1270	arg1	±					1272:1272	±	1272:1272	±	1272:1272	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	5	73	from	operations	844:853	arg1	valorization					791:802	the valorization	787:802	the valorization of underutilized scraps from industrial operations	787:853	Cost could pose a hurdle, eliminated through the valorization of underutilized scraps from industrial operations, which is also in line with the circular bioeconomy in terms of the integral use of biomass.
32935963	5	73	from	operations	844:853	arg1	scraps					821:826	underutilized scraps	807:826	underutilized scraps from industrial operations	807:853	Cost could pose a hurdle, eliminated through the valorization of underutilized scraps from industrial operations, which is also in line with the circular bioeconomy in terms of the integral use of biomass.
32935963	5	74	from	bioeconomy	896:905	arg1	terms					910:914	terms	910:914	terms of the integral use of biomass	910:945	Cost could pose a hurdle, eliminated through the valorization of underutilized scraps from industrial operations, which is also in line with the circular bioeconomy in terms of the integral use of biomass.
32935963	5	75	theme	integral	923:930	arg1	use					932:934	the integral use	919:934	the integral use of biomass	919:945	Cost could pose a hurdle, eliminated through the valorization of underutilized scraps from industrial operations, which is also in line with the circular bioeconomy in terms of the integral use of biomass.
32935963	4	76	theme	packaging	522:530	arg1	material					532:539	a packaging material	520:539	a packaging material	520:539	Optimum performance as a packaging material was achieved by engineering BCNC surface chemistry as well as the filler-in-matrix dispersion, targeting the replacement of unsustainable, fossil-derived plastics intended for single-use applications.
32935963	6	77	theme	0.02	1259:1262	arg1	±					1272:1272	±	1272:1272	±	1272:1272	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	7	78	theme	HPMC	1470:1473	arg1	films					1475:1479	HPMC films	1470:1479	HPMC films	1470:1479	This dual, win-win effect of BCNCs on the mechanical and barrier properties of HPMC films was enabled by a suitable dispersion state, achieved via high-energy mixing, and quenched by casting.
32935963	3	79	theme	all-cellulose	364:376	arg1	composites					378:387	all-cellulose composites	364:387	all-cellulose composites comprising cellulose ether as a matrix and highly pure bacterial cellulose nanocrystals (BCNCs) as fillers	364:494	Inspired by the multiscale architecture of cellulose, we report on all-cellulose composites comprising cellulose ether as a matrix and highly pure bacterial cellulose nanocrystals (BCNCs) as fillers.
32935963	6	80	theme	0.40	1252:1255	arg1	±					1272:1272	±	1272:1272	±	1272:1272	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	8	81	theme	non-renewable	1738:1750	arg1	plastics					1771:1778	non-renewable, non-biodegradable plastics	1738:1778	non-renewable, non-biodegradable plastics in short-term applications	1738:1805	This study adds to the current literature on all-cellulose composites and helps pave the route for the technical and economical feasibilities of replacing non-renewable, non-biodegradable plastics in short-term applications by materials that are both renewable and biodegradable, that are also produced through green protocols and isolated from surplus biomass, and that still perform similarly or even better.
32935963	4	82	theme	Optimum	497:503	arg1	performance					505:515	Optimum performance	497:515	Optimum performance as a packaging material	497:539	Optimum performance as a packaging material was achieved by engineering BCNC surface chemistry as well as the filler-in-matrix dispersion, targeting the replacement of unsustainable, fossil-derived plastics intended for single-use applications.
32935963	3	83	theme	cellulose	454:462	arg1	ether					410:414	cellulose ether	400:414	cellulose ether	400:414	Inspired by the multiscale architecture of cellulose, we report on all-cellulose composites comprising cellulose ether as a matrix and highly pure bacterial cellulose nanocrystals (BCNCs) as fillers.
32935963	3	83	theme	cellulose	454:462	arg1	BCNCs					478:482	BCNCs	478:482	BCNCs	478:482	Inspired by the multiscale architecture of cellulose, we report on all-cellulose composites comprising cellulose ether as a matrix and highly pure bacterial cellulose nanocrystals (BCNCs) as fillers.
32935963	3	83	theme	cellulose	454:462	arg1	nanocrystals					464:475	bacterial cellulose nanocrystals	444:475	highly pure bacterial cellulose nanocrystals (BCNCs)	432:483	Inspired by the multiscale architecture of cellulose, we report on all-cellulose composites comprising cellulose ether as a matrix and highly pure bacterial cellulose nanocrystals (BCNCs) as fillers.
32935963	0	84	theme	Microbial	10:18	arg1	Scraps					30:35	Microbial Cellulose Scraps	10:35	Microbial Cellulose Scraps	10:35	Upcycling Microbial Cellulose Scraps into Nanowhiskers with Engineered Performance as Fillers in All-Cellulose Composites.
32935963	4	85	theme	BCNC	569:572	arg1	chemistry					582:590	BCNC surface chemistry	569:590	BCNC surface chemistry as well as the filler-in-matrix dispersion, targeting the replacement of unsustainable, fossil-derived plastics intended for single-use applications	569:739	Optimum performance as a packaging material was achieved by engineering BCNC surface chemistry as well as the filler-in-matrix dispersion, targeting the replacement of unsustainable, fossil-derived plastics intended for single-use applications.
32935963	3	86	theme	pure	439:442	arg1	ether					410:414	cellulose ether	400:414	cellulose ether	400:414	Inspired by the multiscale architecture of cellulose, we report on all-cellulose composites comprising cellulose ether as a matrix and highly pure bacterial cellulose nanocrystals (BCNCs) as fillers.
32935963	3	86	theme	pure	439:442	arg1	BCNCs					478:482	BCNCs	478:482	BCNCs	478:482	Inspired by the multiscale architecture of cellulose, we report on all-cellulose composites comprising cellulose ether as a matrix and highly pure bacterial cellulose nanocrystals (BCNCs) as fillers.
32935963	3	86	theme	pure	439:442	arg1	nanocrystals					464:475	bacterial cellulose nanocrystals	444:475	highly pure bacterial cellulose nanocrystals (BCNCs)	432:483	Inspired by the multiscale architecture of cellulose, we report on all-cellulose composites comprising cellulose ether as a matrix and highly pure bacterial cellulose nanocrystals (BCNCs) as fillers.
32935963	6	87	theme	crystalline	1373:1383	arg1	nm					1360:1361	reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide	1164:1366	reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide	1164:1366	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	87	theme	crystalline	1373:1383	arg1	BCNC					1385:1388	88% crystalline BCNC	1369:1388	88% crystalline BCNC	1369:1388	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	88	theme	m-2	1288:1290	arg1	permeability					1233:1244	water vapor permeability	1221:1244	water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1)	1221:1297	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	88	theme	m-2	1288:1290	arg1	kPa-1					1292:1296	from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1	1247:1296	from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1	1247:1296	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	89	from	permeability	1233:1244	arg1	elongation					1172:1181	elongation	1172:1181	elongation	1172:1181	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	89	from	permeability	1233:1244	arg1	±					1355:1355	13 ± 3	1352:1357	13 ± 3	1352:1357	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	4	90	dep	unsustainable	665:677	arg1	fossil-derived					680:693	fossil-derived	680:693	fossil-derived	680:693	Optimum performance as a packaging material was achieved by engineering BCNC surface chemistry as well as the filler-in-matrix dispersion, targeting the replacement of unsustainable, fossil-derived plastics intended for single-use applications.
32935963	7	91	theme	BCNCs	1420:1424	arg1	effect					1410:1415	This dual, win-win effect	1391:1415	This dual, win-win effect of BCNCs on the mechanical and barrier properties of HPMC films	1391:1479	This dual, win-win effect of BCNCs on the mechanical and barrier properties of HPMC films was enabled by a suitable dispersion state, achieved via high-energy mixing, and quenched by casting.
32935963	5	92	with	line	873:876	arg1	bioeconomy					896:905	the circular bioeconomy	883:905	the circular bioeconomy in terms of the integral use of biomass	883:945	Cost could pose a hurdle, eliminated through the valorization of underutilized scraps from industrial operations, which is also in line with the circular bioeconomy in terms of the integral use of biomass.
32935963	0	93	theme	Engineered	60:69	arg1	Performance					71:81	Engineered Performance	60:81	Engineered Performance as Fillers in All-Cellulose Composites	60:120	Upcycling Microbial Cellulose Scraps into Nanowhiskers with Engineered Performance as Fillers in All-Cellulose Composites.
32935963	8	94	attach	isolated	1914:1921	arg2	that					1863:1866	that	1863:1866	that	1863:1866	This study adds to the current literature on all-cellulose composites and helps pave the route for the technical and economical feasibilities of replacing non-renewable, non-biodegradable plastics in short-term applications by materials that are both renewable and biodegradable, that are also produced through green protocols and isolated from surplus biomass, and that still perform similarly or even better.
32935963	8	94	attach	isolated	1914:1921	arg1	biomass					1936:1942	surplus biomass	1928:1942	surplus biomass	1928:1942	This study adds to the current literature on all-cellulose composites and helps pave the route for the technical and economical feasibilities of replacing non-renewable, non-biodegradable plastics in short-term applications by materials that are both renewable and biodegradable, that are also produced through green protocols and isolated from surplus biomass, and that still perform similarly or even better.
32935963	8	95	theme	green	1894:1898	arg1	protocols					1900:1908	green protocols	1894:1908	green protocols	1894:1908	This study adds to the current literature on all-cellulose composites and helps pave the route for the technical and economical feasibilities of replacing non-renewable, non-biodegradable plastics in short-term applications by materials that are both renewable and biodegradable, that are also produced through green protocols and isolated from surplus biomass, and that still perform similarly or even better.
32935963	6	96	theme	Young	1108:1112	arg1	modulus					1116:1122	Young's modulus	1108:1122	Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa)	1108:1156	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	0	97	with	Nanowhiskers	42:53	arg1	Performance					71:81	Engineered Performance	60:81	Engineered Performance as Fillers in All-Cellulose Composites	60:120	Upcycling Microbial Cellulose Scraps into Nanowhiskers with Engineered Performance as Fillers in All-Cellulose Composites.
32935963	8	98	theme	short-term	1783:1792	arg1	applications					1794:1805	short-term applications	1783:1805	short-term applications	1783:1805	This study adds to the current literature on all-cellulose composites and helps pave the route for the technical and economical feasibilities of replacing non-renewable, non-biodegradable plastics in short-term applications by materials that are both renewable and biodegradable, that are also produced through green protocols and isolated from surplus biomass, and that still perform similarly or even better.
32935963	6	99	theme	wt	1323:1324	arg1	±					1336:1336	120 ± 31	1332:1339	120 ± 31	1332:1339	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	99	theme	wt	1323:1324	arg1	nm					1342:1343	(120 ± 31) nm	1331:1343	(120 ± 31) nm long	1331:1348	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32935963	6	99	theme	wt	1323:1324	arg1	%					1326:1326	only 5 wt %	1316:1326	only 5 wt % of (120 ± 31) nm long	1316:1348	As far as performance, the optically transparent hydroxypropyl methylcellulose (HPMC) films presented improved tensile strength (from 61 ± 6 to 86 ± 9 MPa) and Young's modulus (from 1.5 ± 0.2 to 2.7 ± 0.4 GPa) while reduced elongation at break (from 15 ± 2 to 12 ± 2%) and water vapor permeability (from 0.40 ± 0.02 to 0.31 ± 0.01 g mm h-1 m-2 kPa-1) when filled with only 5 wt % of (120 ± 31) nm long, (13 ± 3) nm wide, 88% crystalline BCNC.
32066333	10	0	theme	linear	1370:1375	arg1	relationship					1377:1388	piecewise linear relationship	1360:1388	piecewise linear relationship of intensity of RGB to H2O2 concentration	1360:1430	The colour analysis revealed piecewise linear relationship of intensity of RGB to H2O2 concentration in the range of 0.1-1.5 and 2-10 mM with R2 values of 0.97 and 0.9778, respectively.
32066333	8	1	theme	paper	1148:1152	arg1	strips					1154:1159	the decoloured paper strips	1133:1159	the decoloured paper strips	1133:1159	The analysis of the decoloured paper strips was done by a smartphone camera and an RGB Colour Reader application (app) to measure colour intensity.
32066333	2	2	from	research	311:318	arg1	nanotechnology					323:336	nanotechnology	323:336	nanotechnology	323:336	This has led to progressive research in nanotechnology for developing efficient nanomaterials for bio-chemical sensing applications.
32066333	7	3	theme	H2O2	1097:1100	arg1	concentration					1102:1114	H2O2 concentration	1097:1114	H2O2 concentration	1097:1114	Detection of H2O2 is based on the discolouration of the Ag NPs-alginate composite modified paper sensor as a function of H2O2 concentration.
32066333	1	4	theme	last	263:266	arg1	years					276:280	the last several years	259:280	the last several years	259:280	There has been an increase in the discovery and usage of sensors for the detection of chemical compounds in the field of analytical chemistry since the last several years.
32066333	4	5	theme	average	652:658	arg1	analysis					638:645	per the X-ray diffraction analysis	612:645	per the X-ray diffraction analysis	612:645	As per the X-ray diffraction analysis, the average particle size of Ag NPs was found to be 10 nm.
32066333	4	5	theme	average	652:658	arg1	10 nm					700:704	10 nm	700:704	10 nm	700:704	As per the X-ray diffraction analysis, the average particle size of Ag NPs was found to be 10 nm.
32066333	4	5	theme	average	652:658	arg1	size					669:672	the average particle size	648:672	the average particle size of Ag NPs	648:682	As per the X-ray diffraction analysis, the average particle size of Ag NPs was found to be 10 nm.
32066333	6	6	theme	Ag	874:875	arg1	composite					890:898	The synthesized Ag NPs-alginate composite	858:898	The synthesized Ag NPs-alginate composite	858:898	The synthesized Ag NPs-alginate composite was further used to develop a paper-based sensor for the detection of H2O2.
32066333	8	7	theme	smartphone	1175:1184	arg1	camera					1186:1191	a smartphone camera	1173:1191	a smartphone camera	1173:1191	The analysis of the decoloured paper strips was done by a smartphone camera and an RGB Colour Reader application (app) to measure colour intensity.
32066333	4	8	theme	diffraction	626:636	arg1	analysis					638:645	per the X-ray diffraction analysis	612:645	per the X-ray diffraction analysis	612:645	As per the X-ray diffraction analysis, the average particle size of Ag NPs was found to be 10 nm.
32066333	4	8	theme	diffraction	626:636	arg1	size					669:672	the average particle size	648:672	the average particle size of Ag NPs	648:682	As per the X-ray diffraction analysis, the average particle size of Ag NPs was found to be 10 nm.
32066333	7	9	theme	paper	1067:1071	arg1	sensor					1073:1078	the Ag NPs-alginate composite modified paper sensor	1028:1078	the Ag NPs-alginate composite modified paper sensor	1028:1078	Detection of H2O2 is based on the discolouration of the Ag NPs-alginate composite modified paper sensor as a function of H2O2 concentration.
32066333	1	10	theme	chemical	197:204	arg1	compounds					206:214	chemical compounds	197:214	chemical compounds	197:214	There has been an increase in the discovery and usage of sensors for the detection of chemical compounds in the field of analytical chemistry since the last several years.
32066333	7	11	theme	composite	1048:1056	arg1	sensor					1073:1078	the Ag NPs-alginate composite modified paper sensor	1028:1078	the Ag NPs-alginate composite modified paper sensor	1028:1078	Detection of H2O2 is based on the discolouration of the Ag NPs-alginate composite modified paper sensor as a function of H2O2 concentration.
32066333	5	12	theme	374.11 eV	812:820	arg1	energies					789:796	binding energies	781:796	binding energies of 368.10 and 374.11 eV	781:820	X-ray photoelectron spectroscpopy analysis showed characteristic peaks at binding energies of 368.10 and 374.11 eV indicating the formation of Ag NPs.
32066333	5	13	theme	Ag	850:851	arg1	NPs					853:855	Ag NPs	850:855	Ag NPs	850:855	X-ray photoelectron spectroscpopy analysis showed characteristic peaks at binding energies of 368.10 and 374.11 eV indicating the formation of Ag NPs.
32066333	8	14	theme	RGB	1200:1202	arg1	application					1218:1228	an RGB Colour Reader application	1197:1228	an RGB Colour Reader application (app)	1197:1234	The analysis of the decoloured paper strips was done by a smartphone camera and an RGB Colour Reader application (app) to measure colour intensity.
32066333	8	14	theme	RGB	1200:1202	arg1	app					1231:1233	app	1231:1233	app	1231:1233	The analysis of the decoloured paper strips was done by a smartphone camera and an RGB Colour Reader application (app) to measure colour intensity.
32066333	7	15	theme	Ag	1032:1033	arg1	sensor					1073:1078	the Ag NPs-alginate composite modified paper sensor	1028:1078	the Ag NPs-alginate composite modified paper sensor	1028:1078	Detection of H2O2 is based on the discolouration of the Ag NPs-alginate composite modified paper sensor as a function of H2O2 concentration.
32066333	3	16	from	agent	564:568	arg1	approach					599:606	a fast and cost-effective approach	573:606	a fast and cost-effective approach	573:606	Thereby, a deft synthesis of silver nanoparticles (Ag NPs) under microwave irradiation was achieved using sodium alginate as a reducing and capping agent in a fast and cost-effective approach.
32066333	4	17	theme	Ag	677:678	arg1	NPs					680:682	Ag NPs	677:682	Ag NPs	677:682	As per the X-ray diffraction analysis, the average particle size of Ag NPs was found to be 10 nm.
32066333	0	18	theme	synthesized	72:82	arg1	Ag					84:85	synthesized Ag	72:85	synthesized Ag	72:85	Development of a surface-modified paper-based colorimetric sensor using synthesized Ag NPs-alginate composite.
32066333	5	19	theme	binding	781:787	arg1	energies					789:796	binding energies	781:796	binding energies of 368.10 and 374.11 eV	781:820	X-ray photoelectron spectroscpopy analysis showed characteristic peaks at binding energies of 368.10 and 374.11 eV indicating the formation of Ag NPs.
32066333	10	20	theme	concentration	1418:1430	arg1	intensity					1393:1401	intensity	1393:1401	intensity of RGB to H2O2 concentration	1393:1430	The colour analysis revealed piecewise linear relationship of intensity of RGB to H2O2 concentration in the range of 0.1-1.5 and 2-10 mM with R2 values of 0.97 and 0.9778, respectively.
32066333	5	21	theme	X-ray	707:711	arg1	analysis					741:748	X-ray photoelectron spectroscpopy analysis	707:748	X-ray photoelectron spectroscpopy analysis	707:748	X-ray photoelectron spectroscpopy analysis showed characteristic peaks at binding energies of 368.10 and 374.11 eV indicating the formation of Ag NPs.
32066333	9	22	theme	sensing	1269:1275	arg1	characteristics					1277:1291	The sensing characteristics	1265:1291	The sensing characteristics	1265:1291	The sensing characteristics were found in the range of 0.1-10 mM.
32066333	3	23	theme	silver	445:450	arg1	nanoparticles					452:464	silver nanoparticles	445:464	silver nanoparticles (Ag NPs) under microwave irradiation	445:501	Thereby, a deft synthesis of silver nanoparticles (Ag NPs) under microwave irradiation was achieved using sodium alginate as a reducing and capping agent in a fast and cost-effective approach.
32066333	3	23	theme	silver	445:450	arg1	NPs					470:472	Ag NPs	467:472	Ag NPs	467:472	Thereby, a deft synthesis of silver nanoparticles (Ag NPs) under microwave irradiation was achieved using sodium alginate as a reducing and capping agent in a fast and cost-effective approach.
32066333	5	24	theme	spectroscpopy	727:739	arg1	analysis					741:748	X-ray photoelectron spectroscpopy analysis	707:748	X-ray photoelectron spectroscpopy analysis	707:748	X-ray photoelectron spectroscpopy analysis showed characteristic peaks at binding energies of 368.10 and 374.11 eV indicating the formation of Ag NPs.
32066333	2	25	theme	sensing	394:400	arg1	applications					402:413	bio-chemical sensing applications	381:413	bio-chemical sensing applications	381:413	This has led to progressive research in nanotechnology for developing efficient nanomaterials for bio-chemical sensing applications.
32066333	1	26	theme	several	268:274	arg1	years					276:280	the last several years	259:280	the last several years	259:280	There has been an increase in the discovery and usage of sensors for the detection of chemical compounds in the field of analytical chemistry since the last several years.
32066333	1	27	dep	discovery	145:153	arg1	the					141:143	the	141:143	the	141:143	There has been an increase in the discovery and usage of sensors for the detection of chemical compounds in the field of analytical chemistry since the last several years.
32066333	1	28	from	detection	184:192	arg1	field					223:227	the field	219:227	the field of analytical chemistry since the last several years	219:280	There has been an increase in the discovery and usage of sensors for the detection of chemical compounds in the field of analytical chemistry since the last several years.
32066333	6	29	theme	synthesized	862:872	arg1	composite					890:898	The synthesized Ag NPs-alginate composite	858:898	The synthesized Ag NPs-alginate composite	858:898	The synthesized Ag NPs-alginate composite was further used to develop a paper-based sensor for the detection of H2O2.
32066333	8	30	theme	Reader	1211:1216	arg1	application					1218:1228	an RGB Colour Reader application	1197:1228	an RGB Colour Reader application (app)	1197:1234	The analysis of the decoloured paper strips was done by a smartphone camera and an RGB Colour Reader application (app) to measure colour intensity.
32066333	8	30	theme	Reader	1211:1216	arg1	app					1231:1233	app	1231:1233	app	1231:1233	The analysis of the decoloured paper strips was done by a smartphone camera and an RGB Colour Reader application (app) to measure colour intensity.
32066333	9	31	theme	0.1-10 mM	1320:1328	arg1	range					1311:1315	the range	1307:1315	the range of 0.1-10 mM	1307:1328	The sensing characteristics were found in the range of 0.1-10 mM.
32066333	0	32	theme	paper-based	34:44	arg1	sensor					59:64	a surface-modified paper-based colorimetric sensor	15:64	a surface-modified paper-based colorimetric sensor using synthesized Ag	15:85	Development of a surface-modified paper-based colorimetric sensor using synthesized Ag NPs-alginate composite.
32066333	10	33	theme	0.97	1486:1489	arg1	values					1476:1481	R2 values	1473:1481	R2 values	1473:1481	The colour analysis revealed piecewise linear relationship of intensity of RGB to H2O2 concentration in the range of 0.1-1.5 and 2-10 mM with R2 values of 0.97 and 0.9778, respectively.
32066333	0	34	theme	sensor	59:64	arg1	Development					0:10	Development	0:10	Development of a surface-modified paper-based colorimetric sensor using synthesized Ag	0:85	Development of a surface-modified paper-based colorimetric sensor using synthesized Ag NPs-alginate composite.
32066333	11	35	theme	H2O2	1662:1665	arg1	analysis					1650:1657	real-time analysis	1640:1657	real-time analysis of H2O2	1640:1665	Owing to the high sensitivity, selectivity, and cost-effectiveness, the developed paper sensor can be a potential tool for real-time analysis of H2O2.
32066333	3	36	theme	sodium	522:527	arg1	agent					564:568	a reducing and capping agent	541:568	agent	564:568	Thereby, a deft synthesis of silver nanoparticles (Ag NPs) under microwave irradiation was achieved using sodium alginate as a reducing and capping agent in a fast and cost-effective approach.
32066333	3	36	theme	sodium	522:527	arg1	alginate					529:536	sodium alginate	522:536	sodium alginate	522:536	Thereby, a deft synthesis of silver nanoparticles (Ag NPs) under microwave irradiation was achieved using sodium alginate as a reducing and capping agent in a fast and cost-effective approach.
32066333	4	37	theme	per	612:614	arg1	analysis					638:645	per the X-ray diffraction analysis	612:645	per the X-ray diffraction analysis	612:645	As per the X-ray diffraction analysis, the average particle size of Ag NPs was found to be 10 nm.
32066333	4	37	theme	per	612:614	arg1	size					669:672	the average particle size	648:672	the average particle size of Ag NPs	648:682	As per the X-ray diffraction analysis, the average particle size of Ag NPs was found to be 10 nm.
32066333	10	38	theme	0.9778	1495:1500	arg1	values					1476:1481	R2 values	1473:1481	R2 values	1473:1481	The colour analysis revealed piecewise linear relationship of intensity of RGB to H2O2 concentration in the range of 0.1-1.5 and 2-10 mM with R2 values of 0.97 and 0.9778, respectively.
32066333	7	39	theme	concentration	1102:1114	arg1	function					1085:1092	a function	1083:1092	a function of H2O2 concentration	1083:1114	Detection of H2O2 is based on the discolouration of the Ag NPs-alginate composite modified paper sensor as a function of H2O2 concentration.
32066333	2	40	theme	efficient	353:361	arg1	nanomaterials					363:375	efficient nanomaterials	353:375	efficient nanomaterials for bio-chemical sensing applications	353:413	This has led to progressive research in nanotechnology for developing efficient nanomaterials for bio-chemical sensing applications.
32066333	1	41	theme	chemistry	243:251	arg1	field					223:227	the field	219:227	the field of analytical chemistry since the last several years	219:280	There has been an increase in the discovery and usage of sensors for the detection of chemical compounds in the field of analytical chemistry since the last several years.
32066333	11	42	theme	developed	1589:1597	arg1	tool					1631:1634	a potential tool	1619:1634	a potential tool for real-time analysis of H2O2	1619:1665	Owing to the high sensitivity, selectivity, and cost-effectiveness, the developed paper sensor can be a potential tool for real-time analysis of H2O2.
32066333	11	42	theme	developed	1589:1597	arg1	sensor					1605:1610	the developed paper sensor	1585:1610	the developed paper sensor	1585:1610	Owing to the high sensitivity, selectivity, and cost-effectiveness, the developed paper sensor can be a potential tool for real-time analysis of H2O2.
32066333	10	43	theme	piecewise	1360:1368	arg1	relationship					1377:1388	piecewise linear relationship	1360:1388	piecewise linear relationship of intensity of RGB to H2O2 concentration	1360:1430	The colour analysis revealed piecewise linear relationship of intensity of RGB to H2O2 concentration in the range of 0.1-1.5 and 2-10 mM with R2 values of 0.97 and 0.9778, respectively.
32066333	3	44	theme	reducing	543:550	arg1	agent					564:568	a reducing and capping agent	541:568	agent	564:568	Thereby, a deft synthesis of silver nanoparticles (Ag NPs) under microwave irradiation was achieved using sodium alginate as a reducing and capping agent in a fast and cost-effective approach.
32066333	3	44	theme	reducing	543:550	arg1	alginate					529:536	sodium alginate	522:536	sodium alginate	522:536	Thereby, a deft synthesis of silver nanoparticles (Ag NPs) under microwave irradiation was achieved using sodium alginate as a reducing and capping agent in a fast and cost-effective approach.
32066333	8	45	theme	decoloured	1137:1146	arg1	strips					1154:1159	the decoloured paper strips	1133:1159	the decoloured paper strips	1133:1159	The analysis of the decoloured paper strips was done by a smartphone camera and an RGB Colour Reader application (app) to measure colour intensity.
32066333	11	46	theme	potential	1621:1629	arg1	tool					1631:1634	a potential tool	1619:1634	a potential tool for real-time analysis of H2O2	1619:1665	Owing to the high sensitivity, selectivity, and cost-effectiveness, the developed paper sensor can be a potential tool for real-time analysis of H2O2.
32066333	11	46	theme	potential	1621:1629	arg1	sensor					1605:1610	the developed paper sensor	1585:1610	the developed paper sensor	1585:1610	Owing to the high sensitivity, selectivity, and cost-effectiveness, the developed paper sensor can be a potential tool for real-time analysis of H2O2.
32066333	1	47	theme	sensors	168:174	arg1	usage					159:163	usage	159:163	usage	159:163	There has been an increase in the discovery and usage of sensors for the detection of chemical compounds in the field of analytical chemistry since the last several years.
32066333	1	47	theme	sensors	168:174	arg1	discovery					145:153	discovery	145:153	discovery	145:153	There has been an increase in the discovery and usage of sensors for the detection of chemical compounds in the field of analytical chemistry since the last several years.
32066333	8	48	theme	strips	1154:1159	arg1	analysis					1121:1128	The analysis	1117:1128	The analysis of the decoloured paper strips	1117:1159	The analysis of the decoloured paper strips was done by a smartphone camera and an RGB Colour Reader application (app) to measure colour intensity.
32066333	6	49	theme	NPs-alginate	877:888	arg1	composite					890:898	The synthesized Ag NPs-alginate composite	858:898	The synthesized Ag NPs-alginate composite	858:898	The synthesized Ag NPs-alginate composite was further used to develop a paper-based sensor for the detection of H2O2.
32066333	8	50	theme	Colour	1204:1209	arg1	application					1218:1228	an RGB Colour Reader application	1197:1228	an RGB Colour Reader application (app)	1197:1234	The analysis of the decoloured paper strips was done by a smartphone camera and an RGB Colour Reader application (app) to measure colour intensity.
32066333	8	50	theme	Colour	1204:1209	arg1	app					1231:1233	app	1231:1233	app	1231:1233	The analysis of the decoloured paper strips was done by a smartphone camera and an RGB Colour Reader application (app) to measure colour intensity.
32066333	7	51	theme	sensor	1073:1078	arg1	discolouration					1010:1023	the discolouration	1006:1023	the discolouration of the Ag NPs-alginate composite modified paper sensor as a function of H2O2 concentration	1006:1114	Detection of H2O2 is based on the discolouration of the Ag NPs-alginate composite modified paper sensor as a function of H2O2 concentration.
32066333	10	52	theme	intensity	1393:1401	arg1	relationship					1377:1388	piecewise linear relationship	1360:1388	piecewise linear relationship of intensity of RGB to H2O2 concentration	1360:1430	The colour analysis revealed piecewise linear relationship of intensity of RGB to H2O2 concentration in the range of 0.1-1.5 and 2-10 mM with R2 values of 0.97 and 0.9778, respectively.
32066333	2	53	theme	progressive	299:309	arg1	research					311:318	progressive research	299:318	progressive research in nanotechnology for developing efficient nanomaterials for bio-chemical sensing applications	299:413	This has led to progressive research in nanotechnology for developing efficient nanomaterials for bio-chemical sensing applications.
32066333	6	54	theme	H2O2	970:973	arg1	detection					957:965	the detection	953:965	the detection of H2O2	953:973	The synthesized Ag NPs-alginate composite was further used to develop a paper-based sensor for the detection of H2O2.
32066333	4	55	theme	particle	660:667	arg1	analysis					638:645	per the X-ray diffraction analysis	612:645	per the X-ray diffraction analysis	612:645	As per the X-ray diffraction analysis, the average particle size of Ag NPs was found to be 10 nm.
32066333	4	55	theme	particle	660:667	arg1	10 nm					700:704	10 nm	700:704	10 nm	700:704	As per the X-ray diffraction analysis, the average particle size of Ag NPs was found to be 10 nm.
32066333	4	55	theme	particle	660:667	arg1	size					669:672	the average particle size	648:672	the average particle size of Ag NPs	648:682	As per the X-ray diffraction analysis, the average particle size of Ag NPs was found to be 10 nm.
32066333	7	56	theme	modified	1058:1065	arg1	sensor					1073:1078	the Ag NPs-alginate composite modified paper sensor	1028:1078	the Ag NPs-alginate composite modified paper sensor	1028:1078	Detection of H2O2 is based on the discolouration of the Ag NPs-alginate composite modified paper sensor as a function of H2O2 concentration.
32066333	3	57	theme	capping	556:562	arg1	agent					564:568	a reducing and capping agent	541:568	agent	564:568	Thereby, a deft synthesis of silver nanoparticles (Ag NPs) under microwave irradiation was achieved using sodium alginate as a reducing and capping agent in a fast and cost-effective approach.
32066333	3	57	theme	capping	556:562	arg1	alginate					529:536	sodium alginate	522:536	sodium alginate	522:536	Thereby, a deft synthesis of silver nanoparticles (Ag NPs) under microwave irradiation was achieved using sodium alginate as a reducing and capping agent in a fast and cost-effective approach.
32066333	9	58	located	found	1298:1302	arg1	range					1311:1315	the range	1307:1315	the range of 0.1-10 mM	1307:1328	The sensing characteristics were found in the range of 0.1-10 mM.
32066333	9	58	located	found	1298:1302	arg2	characteristics					1277:1291	The sensing characteristics	1265:1291	The sensing characteristics	1265:1291	The sensing characteristics were found in the range of 0.1-10 mM.
32066333	4	59	theme	X-ray	620:624	arg1	analysis					638:645	per the X-ray diffraction analysis	612:645	per the X-ray diffraction analysis	612:645	As per the X-ray diffraction analysis, the average particle size of Ag NPs was found to be 10 nm.
32066333	4	59	theme	X-ray	620:624	arg1	size					669:672	the average particle size	648:672	the average particle size of Ag NPs	648:682	As per the X-ray diffraction analysis, the average particle size of Ag NPs was found to be 10 nm.
32066333	7	60	theme	NPs-alginate	1035:1046	arg1	sensor					1073:1078	the Ag NPs-alginate composite modified paper sensor	1028:1078	the Ag NPs-alginate composite modified paper sensor	1028:1078	Detection of H2O2 is based on the discolouration of the Ag NPs-alginate composite modified paper sensor as a function of H2O2 concentration.
32066333	3	61	theme	cost-effective	584:597	arg1	approach					599:606	a fast and cost-effective approach	573:606	a fast and cost-effective approach	573:606	Thereby, a deft synthesis of silver nanoparticles (Ag NPs) under microwave irradiation was achieved using sodium alginate as a reducing and capping agent in a fast and cost-effective approach.
32066333	1	62	theme	compounds	206:214	arg1	detection					184:192	the detection	180:192	the detection of chemical compounds in the field of analytical chemistry since the last several years	180:280	There has been an increase in the discovery and usage of sensors for the detection of chemical compounds in the field of analytical chemistry since the last several years.
32066333	3	63	theme	fast	575:578	arg1	approach					599:606	a fast and cost-effective approach	573:606	a fast and cost-effective approach	573:606	Thereby, a deft synthesis of silver nanoparticles (Ag NPs) under microwave irradiation was achieved using sodium alginate as a reducing and capping agent in a fast and cost-effective approach.
32066333	6	64	theme	paper-based	930:940	arg1	sensor					942:947	a paper-based sensor	928:947	a paper-based sensor for the detection of H2O2	928:973	The synthesized Ag NPs-alginate composite was further used to develop a paper-based sensor for the detection of H2O2.
32066333	8	65	theme	colour	1247:1252	arg1	intensity					1254:1262	colour intensity	1247:1262	colour intensity	1247:1262	The analysis of the decoloured paper strips was done by a smartphone camera and an RGB Colour Reader application (app) to measure colour intensity.
32066333	7	66	theme	H2O2	989:992	arg1	Detection					976:984	Detection	976:984	Detection of H2O2	976:992	Detection of H2O2 is based on the discolouration of the Ag NPs-alginate composite modified paper sensor as a function of H2O2 concentration.
32066333	6	67	used	used	912:915	arg2	composite					890:898	The synthesized Ag NPs-alginate composite	858:898	The synthesized Ag NPs-alginate composite	858:898	The synthesized Ag NPs-alginate composite was further used to develop a paper-based sensor for the detection of H2O2.
32066333	5	68	theme	characteristic	757:770	arg1	peaks					772:776	characteristic peaks	757:776	characteristic peaks	757:776	X-ray photoelectron spectroscpopy analysis showed characteristic peaks at binding energies of 368.10 and 374.11 eV indicating the formation of Ag NPs.
32066333	4	69	theme	NPs	680:682	arg1	analysis					638:645	per the X-ray diffraction analysis	612:645	per the X-ray diffraction analysis	612:645	As per the X-ray diffraction analysis, the average particle size of Ag NPs was found to be 10 nm.
32066333	4	69	theme	NPs	680:682	arg1	10 nm					700:704	10 nm	700:704	10 nm	700:704	As per the X-ray diffraction analysis, the average particle size of Ag NPs was found to be 10 nm.
32066333	4	69	theme	NPs	680:682	arg1	size					669:672	the average particle size	648:672	the average particle size of Ag NPs	648:682	As per the X-ray diffraction analysis, the average particle size of Ag NPs was found to be 10 nm.
32066333	10	70	theme	RGB	1406:1408	arg1	concentration					1418:1430	RGB to H2O2 concentration	1406:1430	RGB to H2O2 concentration	1406:1430	The colour analysis revealed piecewise linear relationship of intensity of RGB to H2O2 concentration in the range of 0.1-1.5 and 2-10 mM with R2 values of 0.97 and 0.9778, respectively.
32066333	5	71	theme	NPs	853:855	arg1	formation					837:845	the formation	833:845	the formation of Ag NPs	833:855	X-ray photoelectron spectroscpopy analysis showed characteristic peaks at binding energies of 368.10 and 374.11 eV indicating the formation of Ag NPs.
32066333	5	72	theme	photoelectron	713:725	arg1	analysis					741:748	X-ray photoelectron spectroscpopy analysis	707:748	X-ray photoelectron spectroscpopy analysis	707:748	X-ray photoelectron spectroscpopy analysis showed characteristic peaks at binding energies of 368.10 and 374.11 eV indicating the formation of Ag NPs.
32066333	3	73	theme	nanoparticles	452:464	arg1	synthesis					432:440	a deft synthesis	425:440	a deft synthesis of silver nanoparticles (Ag NPs) under microwave irradiation	425:501	Thereby, a deft synthesis of silver nanoparticles (Ag NPs) under microwave irradiation was achieved using sodium alginate as a reducing and capping agent in a fast and cost-effective approach.
32066333	5	74	theme	368.10	801:806	arg1	energies					789:796	binding energies	781:796	binding energies of 368.10 and 374.11 eV	781:820	X-ray photoelectron spectroscpopy analysis showed characteristic peaks at binding energies of 368.10 and 374.11 eV indicating the formation of Ag NPs.
32066333	2	75	theme	bio-chemical	381:392	arg1	applications					402:413	bio-chemical sensing applications	381:413	bio-chemical sensing applications	381:413	This has led to progressive research in nanotechnology for developing efficient nanomaterials for bio-chemical sensing applications.
32066333	0	76	theme	surface-modified	17:32	arg1	sensor					59:64	a surface-modified paper-based colorimetric sensor	15:64	a surface-modified paper-based colorimetric sensor using synthesized Ag	15:85	Development of a surface-modified paper-based colorimetric sensor using synthesized Ag NPs-alginate composite.
32066333	1	77	from	increase	129:136	arg1	usage					159:163	usage	159:163	usage	159:163	There has been an increase in the discovery and usage of sensors for the detection of chemical compounds in the field of analytical chemistry since the last several years.
32066333	1	77	from	increase	129:136	arg1	discovery					145:153	discovery	145:153	discovery	145:153	There has been an increase in the discovery and usage of sensors for the detection of chemical compounds in the field of analytical chemistry since the last several years.
32066333	10	78	theme	2-10 mM	1460:1466	arg1	range					1439:1443	the range	1435:1443	the range of 0.1-1.5 and 2-10 mM	1435:1466	The colour analysis revealed piecewise linear relationship of intensity of RGB to H2O2 concentration in the range of 0.1-1.5 and 2-10 mM with R2 values of 0.97 and 0.9778, respectively.
32066333	11	79	theme	real-time	1640:1648	arg1	analysis					1650:1657	real-time analysis	1640:1657	real-time analysis of H2O2	1640:1665	Owing to the high sensitivity, selectivity, and cost-effectiveness, the developed paper sensor can be a potential tool for real-time analysis of H2O2.
32066333	0	80	theme	colorimetric	46:57	arg1	sensor					59:64	a surface-modified paper-based colorimetric sensor	15:64	a surface-modified paper-based colorimetric sensor using synthesized Ag	15:85	Development of a surface-modified paper-based colorimetric sensor using synthesized Ag NPs-alginate composite.
32066333	3	81	theme	deft	427:430	arg1	synthesis					432:440	a deft synthesis	425:440	a deft synthesis of silver nanoparticles (Ag NPs) under microwave irradiation	425:501	Thereby, a deft synthesis of silver nanoparticles (Ag NPs) under microwave irradiation was achieved using sodium alginate as a reducing and capping agent in a fast and cost-effective approach.
32066333	10	82	theme	0.1-1.5	1448:1454	arg1	range					1439:1443	the range	1435:1443	the range of 0.1-1.5 and 2-10 mM	1435:1466	The colour analysis revealed piecewise linear relationship of intensity of RGB to H2O2 concentration in the range of 0.1-1.5 and 2-10 mM with R2 values of 0.97 and 0.9778, respectively.
32066333	3	83	theme	Ag	467:468	arg1	nanoparticles					452:464	silver nanoparticles	445:464	silver nanoparticles (Ag NPs) under microwave irradiation	445:501	Thereby, a deft synthesis of silver nanoparticles (Ag NPs) under microwave irradiation was achieved using sodium alginate as a reducing and capping agent in a fast and cost-effective approach.
32066333	3	83	theme	Ag	467:468	arg1	NPs					470:472	Ag NPs	467:472	Ag NPs	467:472	Thereby, a deft synthesis of silver nanoparticles (Ag NPs) under microwave irradiation was achieved using sodium alginate as a reducing and capping agent in a fast and cost-effective approach.
32066333	11	84	theme	high	1530:1533	arg1	sensitivity					1535:1545	the high sensitivity	1526:1545	the high sensitivity	1526:1545	Owing to the high sensitivity, selectivity, and cost-effectiveness, the developed paper sensor can be a potential tool for real-time analysis of H2O2.
32066333	10	85	theme	R2	1473:1474	arg1	values					1476:1481	R2 values	1473:1481	R2 values	1473:1481	The colour analysis revealed piecewise linear relationship of intensity of RGB to H2O2 concentration in the range of 0.1-1.5 and 2-10 mM with R2 values of 0.97 and 0.9778, respectively.
32066333	10	86	theme	colour	1335:1340	arg1	analysis					1342:1349	The colour analysis	1331:1349	The colour analysis	1331:1349	The colour analysis revealed piecewise linear relationship of intensity of RGB to H2O2 concentration in the range of 0.1-1.5 and 2-10 mM with R2 values of 0.97 and 0.9778, respectively.
32066333	3	87	theme	microwave	481:489	arg1	irradiation					491:501	microwave irradiation	481:501	microwave irradiation	481:501	Thereby, a deft synthesis of silver nanoparticles (Ag NPs) under microwave irradiation was achieved using sodium alginate as a reducing and capping agent in a fast and cost-effective approach.
32066333	11	88	theme	paper	1599:1603	arg1	tool					1631:1634	a potential tool	1619:1634	a potential tool for real-time analysis of H2O2	1619:1665	Owing to the high sensitivity, selectivity, and cost-effectiveness, the developed paper sensor can be a potential tool for real-time analysis of H2O2.
32066333	11	88	theme	paper	1599:1603	arg1	sensor					1605:1610	the developed paper sensor	1585:1610	the developed paper sensor	1585:1610	Owing to the high sensitivity, selectivity, and cost-effectiveness, the developed paper sensor can be a potential tool for real-time analysis of H2O2.
32066333	1	89	theme	analytical	232:241	arg1	chemistry					243:251	analytical chemistry	232:251	analytical chemistry	232:251	There has been an increase in the discovery and usage of sensors for the detection of chemical compounds in the field of analytical chemistry since the last several years.
33748076	8	0	theme	distinct	1130:1137	arg1	difference					1139:1148	the distinct difference	1126:1148	the distinct difference between ESCC patients and HVs	1126:1178	Datura stramonium (DSA) was selected to isolate the GlcNAc or Galβ1-4GlcNA-containing glycoproteins due to the distinct difference between ESCC patients and HVs.
33748076	12	1	theme	N-glycans	1770:1778	arg1	proportion					1717:1726	the proportion	1713:1726	the proportion of the GlcNAc or Galβ1-4GlcNAc-containing N-glycans	1713:1778	The MALDI-TOF/TOF-MS results indicated that the proportion of the GlcNAc or Galβ1-4GlcNAc-containing N-glycans was increased in ESCC patients (79.04%) compared with HV (63.20%), which was consistent with the results of lectin microarrays.
33748076	14	2	theme	ESCC	2188:2191	arg1	patients					2193:2200	ESCC patients	2188:2200	ESCC patients	2188:2200	And the altered salivary glycopatterns such as GlcNAc or Galβ1-4GlcNAc-containing N-glycans recognized by DSA might serve as potential biomarkers for the diagnosis of ESCC patients.
33748076	4	3	theme	squamous	417:424	arg1	histology					476:484	the primary pathological histology	451:484	the primary pathological histology in developing countries	451:508	Esophageal squamous cell carcinoma (ESCC) is the primary pathological histology in developing countries and a severe threat to human health.
33748076	4	3	theme	squamous	417:424	arg1	ESCC					442:445	ESCC	442:445	ESCC	442:445	Esophageal squamous cell carcinoma (ESCC) is the primary pathological histology in developing countries and a severe threat to human health.
33748076	4	3	theme	squamous	417:424	arg1	carcinoma					431:439	Esophageal squamous cell carcinoma	406:439	Esophageal squamous cell carcinoma (ESCC)	406:446	Esophageal squamous cell carcinoma (ESCC) is the primary pathological histology in developing countries and a severe threat to human health.
33748076	11	4	dep	GalNAc	1549:1554	arg1	profiles					1600:1607	expression profiles	1589:1607	expression profiles	1589:1607	The ESCC patients showed higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles and lower levels of mannose and fucose expression profiles.
33748076	11	5	theme	higher	1532:1537	arg1	levels					1539:1544	higher levels	1532:1544	higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles	1532:1607	The ESCC patients showed higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles and lower levels of mannose and fucose expression profiles.
33748076	6	6	theme	=	852:852	arg1	n					850:850	n = 25	850:855	n = 25	850:855	In the present study, ESCC patients (n = 16) and healthy volunteers (HVs, n = 25) were enrolled.
33748076	14	7	theme	potential	2146:2154	arg1	N-glycans					2103:2111	Galβ1-4GlcNAc-containing N-glycans	2078:2111	Galβ1-4GlcNAc-containing N-glycans	2078:2111	And the altered salivary glycopatterns such as GlcNAc or Galβ1-4GlcNAc-containing N-glycans recognized by DSA might serve as potential biomarkers for the diagnosis of ESCC patients.
33748076	14	7	theme	potential	2146:2154	arg1	glycopatterns					2046:2058	the altered salivary glycopatterns	2025:2058	the altered salivary glycopatterns such as GlcNAc or Galβ1-4GlcNAc-containing N-glycans recognized by DSA	2025:2129	And the altered salivary glycopatterns such as GlcNAc or Galβ1-4GlcNAc-containing N-glycans recognized by DSA might serve as potential biomarkers for the diagnosis of ESCC patients.
33748076	14	7	theme	potential	2146:2154	arg1	GlcNAc					2068:2073	GlcNAc	2068:2073	GlcNAc	2068:2073	And the altered salivary glycopatterns such as GlcNAc or Galβ1-4GlcNAc-containing N-glycans recognized by DSA might serve as potential biomarkers for the diagnosis of ESCC patients.
33748076	14	7	theme	potential	2146:2154	arg1	biomarkers					2156:2165	potential biomarkers	2146:2165	potential biomarkers for the diagnosis of ESCC patients	2146:2200	And the altered salivary glycopatterns such as GlcNAc or Galβ1-4GlcNAc-containing N-glycans recognized by DSA might serve as potential biomarkers for the diagnosis of ESCC patients.
33748076	9	8	theme	DSA-enriched	1200:1211	arg1	glycoproteins					1213:1225	DSA-enriched glycoproteins	1200:1225	DSA-enriched glycoproteins	1200:1225	The N-glycans from DSA-enriched glycoproteins were released by PNGase F and further identified by MALDI-TOF/TOF-MS to obtain the precise structural information of the altered glycans.
33748076	1	9	theme	remarkable	200:209	arg1	amount					211:216	a remarkable amount	198:216	a remarkable amount of biological information	198:242	Glycosylation is one of the most crucial posttranslational modifications of proteins, containing a remarkable amount of biological information.
33748076	1	9	theme	remarkable	200:209	arg1	information					232:242	biological information	221:242	biological information	221:242	Glycosylation is one of the most crucial posttranslational modifications of proteins, containing a remarkable amount of biological information.
33748076	4	10	from	histology	476:484	arg1	countries					500:508	developing countries	489:508	developing countries	489:508	Esophageal squamous cell carcinoma (ESCC) is the primary pathological histology in developing countries and a severe threat to human health.
33748076	4	11	theme	primary	455:461	arg1	carcinoma					431:439	Esophageal squamous cell carcinoma	406:439	Esophageal squamous cell carcinoma (ESCC)	406:446	Esophageal squamous cell carcinoma (ESCC) is the primary pathological histology in developing countries and a severe threat to human health.
33748076	4	11	theme	primary	455:461	arg1	histology					476:484	the primary pathological histology	451:484	the primary pathological histology in developing countries	451:508	Esophageal squamous cell carcinoma (ESCC) is the primary pathological histology in developing countries and a severe threat to human health.
33748076	3	12	from	topic	383:387	arg1	years					399:403	recent years	392:403	recent years	392:403	Exploring glyco-code in the development of diseases is a hot topic in recent years.
33748076	7	13	theme	altered	987:993	arg1	glycopatterns					1004:1016	the altered salivary glycopatterns	983:1016	the altered salivary glycopatterns	983:1016	The glycomic strategy combining lectin microarray and lectin blotting was employed to investigate and confirm the altered salivary glycopatterns.
33748076	1	14	theme	information	232:242	arg1	amount					211:216	a remarkable amount	198:216	a remarkable amount of biological information	198:242	Glycosylation is one of the most crucial posttranslational modifications of proteins, containing a remarkable amount of biological information.
33748076	1	14	theme	information	232:242	arg1	information					232:242	biological information	221:242	biological information	221:242	Glycosylation is one of the most crucial posttranslational modifications of proteins, containing a remarkable amount of biological information.
33748076	9	15	gly	glycoproteins	1213:1225	arg1	glycoproteins					1213:1225	DSA-enriched glycoproteins	1200:1225	DSA-enriched glycoproteins	1200:1225	The N-glycans from DSA-enriched glycoproteins were released by PNGase F and further identified by MALDI-TOF/TOF-MS to obtain the precise structural information of the altered glycans.
33748076	5	16	theme	glycopatterns	698:710	arg1	explicit					762:769	explicit	762:769	explicit	762:769	Although the glycan profiles in the blood samples of ESCC patients were analyzed using glycomic and glycoproteomic methods, the difference of salivary glycopatterns between healthy subjects and ESCC patients is not explicit yet.
33748076	5	16	theme	glycopatterns	698:710	arg1	difference					675:684	the difference	671:684	the difference of salivary glycopatterns between healthy subjects and ESCC patients	671:753	Although the glycan profiles in the blood samples of ESCC patients were analyzed using glycomic and glycoproteomic methods, the difference of salivary glycopatterns between healthy subjects and ESCC patients is not explicit yet.
33748076	11	17	theme	ESCC	1511:1514	arg1	patients					1516:1523	The ESCC patients	1507:1523	The ESCC patients	1507:1523	The ESCC patients showed higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles and lower levels of mannose and fucose expression profiles.
33748076	5	18	theme	healthy	720:726	arg1	subjects					728:735	healthy subjects	720:735	healthy subjects	720:735	Although the glycan profiles in the blood samples of ESCC patients were analyzed using glycomic and glycoproteomic methods, the difference of salivary glycopatterns between healthy subjects and ESCC patients is not explicit yet.
33748076	1	19	theme	crucial	134:140	arg1	modifications					160:172	the most crucial posttranslational modifications	125:172	the most crucial posttranslational modifications of proteins	125:184	Glycosylation is one of the most crucial posttranslational modifications of proteins, containing a remarkable amount of biological information.
33748076	12	20	theme	MALDI-TOF/TOF-MS	1673:1688	arg1	results					1690:1696	The MALDI-TOF/TOF-MS results	1669:1696	The MALDI-TOF/TOF-MS results	1669:1696	The MALDI-TOF/TOF-MS results indicated that the proportion of the GlcNAc or Galβ1-4GlcNAc-containing N-glycans was increased in ESCC patients (79.04%) compared with HV (63.20%), which was consistent with the results of lectin microarrays.
33748076	3	21	theme	diseases	365:372	arg1	development					350:360	the development	346:360	the development of diseases	346:372	Exploring glyco-code in the development of diseases is a hot topic in recent years.
33748076	0	22	theme	Squamous	67:74	arg1	Carcinoma					81:89	Esophageal Squamous Cell Carcinoma	56:89	Esophageal Squamous Cell Carcinoma Patients	56:98	The Abnormal Glycopatterns of Salivary Glycoproteins in Esophageal Squamous Cell Carcinoma Patients.
33748076	2	23	theme	glycosylation	263:275	arg1	alteration					249:258	The alteration	245:258	The alteration of glycosylation	245:275	The alteration of glycosylation is closely associated with certain diseases.
33748076	1	24	theme	modifications	160:172	arg1	modifications					160:172	the most crucial posttranslational modifications	125:172	the most crucial posttranslational modifications of proteins	125:184	Glycosylation is one of the most crucial posttranslational modifications of proteins, containing a remarkable amount of biological information.
33748076	1	24	theme	modifications	160:172	arg1	one					118:120	one	118:120	one	118:120	Glycosylation is one of the most crucial posttranslational modifications of proteins, containing a remarkable amount of biological information.
33748076	0	25	from	Glycopatterns	13:25	arg1	Patients					91:98	Esophageal Squamous Cell Carcinoma Patients	56:98	Esophageal Squamous Cell Carcinoma Patients	56:98	The Abnormal Glycopatterns of Salivary Glycoproteins in Esophageal Squamous Cell Carcinoma Patients.
33748076	0	26	theme	Carcinoma	81:89	arg1	Patients					91:98	Esophageal Squamous Cell Carcinoma Patients	56:98	Esophageal Squamous Cell Carcinoma Patients	56:98	The Abnormal Glycopatterns of Salivary Glycoproteins in Esophageal Squamous Cell Carcinoma Patients.
33748076	5	27	theme	patients	605:612	arg1	samples					589:595	the blood samples	579:595	the blood samples of ESCC patients	579:612	Although the glycan profiles in the blood samples of ESCC patients were analyzed using glycomic and glycoproteomic methods, the difference of salivary glycopatterns between healthy subjects and ESCC patients is not explicit yet.
33748076	1	28	theme	proteins	177:184	arg1	modifications					160:172	the most crucial posttranslational modifications	125:172	the most crucial posttranslational modifications of proteins	125:184	Glycosylation is one of the most crucial posttranslational modifications of proteins, containing a remarkable amount of biological information.
33748076	8	29	theme	Datura	1019:1024	arg1	DSA					1038:1040	DSA	1038:1040	DSA	1038:1040	Datura stramonium (DSA) was selected to isolate the GlcNAc or Galβ1-4GlcNA-containing glycoproteins due to the distinct difference between ESCC patients and HVs.
33748076	8	29	theme	Datura	1019:1024	arg1	stramonium					1026:1035	Datura stramonium	1019:1035	Datura stramonium (DSA)	1019:1041	Datura stramonium (DSA) was selected to isolate the GlcNAc or Galβ1-4GlcNA-containing glycoproteins due to the distinct difference between ESCC patients and HVs.
33748076	4	30	theme	developing	489:498	arg1	countries					500:508	developing countries	489:508	developing countries	489:508	Esophageal squamous cell carcinoma (ESCC) is the primary pathological histology in developing countries and a severe threat to human health.
33748076	9	31	theme	structural	1318:1327	arg1	information					1329:1339	the precise structural information	1306:1339	the precise structural information of the altered glycans	1306:1362	The N-glycans from DSA-enriched glycoproteins were released by PNGase F and further identified by MALDI-TOF/TOF-MS to obtain the precise structural information of the altered glycans.
33748076	5	32	theme	blood	583:587	arg1	samples					589:595	the blood samples	579:595	the blood samples of ESCC patients	579:612	Although the glycan profiles in the blood samples of ESCC patients were analyzed using glycomic and glycoproteomic methods, the difference of salivary glycopatterns between healthy subjects and ESCC patients is not explicit yet.
33748076	3	33	theme	recent	392:397	arg1	years					399:403	recent years	392:403	recent years	392:403	Exploring glyco-code in the development of diseases is a hot topic in recent years.
33748076	14	34	theme	altered	2029:2035	arg1	N-glycans					2103:2111	Galβ1-4GlcNAc-containing N-glycans	2078:2111	Galβ1-4GlcNAc-containing N-glycans	2078:2111	And the altered salivary glycopatterns such as GlcNAc or Galβ1-4GlcNAc-containing N-glycans recognized by DSA might serve as potential biomarkers for the diagnosis of ESCC patients.
33748076	14	34	theme	altered	2029:2035	arg1	glycopatterns					2046:2058	the altered salivary glycopatterns	2025:2058	the altered salivary glycopatterns such as GlcNAc or Galβ1-4GlcNAc-containing N-glycans recognized by DSA	2025:2129	And the altered salivary glycopatterns such as GlcNAc or Galβ1-4GlcNAc-containing N-glycans recognized by DSA might serve as potential biomarkers for the diagnosis of ESCC patients.
33748076	14	34	theme	altered	2029:2035	arg1	GlcNAc					2068:2073	GlcNAc	2068:2073	GlcNAc	2068:2073	And the altered salivary glycopatterns such as GlcNAc or Galβ1-4GlcNAc-containing N-glycans recognized by DSA might serve as potential biomarkers for the diagnosis of ESCC patients.
33748076	14	34	theme	altered	2029:2035	arg1	biomarkers					2156:2165	potential biomarkers	2146:2165	potential biomarkers for the diagnosis of ESCC patients	2146:2200	And the altered salivary glycopatterns such as GlcNAc or Galβ1-4GlcNAc-containing N-glycans recognized by DSA might serve as potential biomarkers for the diagnosis of ESCC patients.
33748076	9	35	theme	altered	1348:1354	arg1	glycans					1356:1362	the altered glycans	1344:1362	the altered glycans	1344:1362	The N-glycans from DSA-enriched glycoproteins were released by PNGase F and further identified by MALDI-TOF/TOF-MS to obtain the precise structural information of the altered glycans.
33748076	12	36	dep	increased	1784:1792	arg1	compared					1820:1827	compared	1820:1827	compared with HV (63.20%), which was consistent with the results of lectin microarrays	1820:1905	The MALDI-TOF/TOF-MS results indicated that the proportion of the GlcNAc or Galβ1-4GlcNAc-containing N-glycans was increased in ESCC patients (79.04%) compared with HV (63.20%), which was consistent with the results of lectin microarrays.
33748076	11	37	theme	expression	1648:1657	arg1	profiles					1659:1666	expression profiles	1648:1666	expression profiles	1648:1666	The ESCC patients showed higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles and lower levels of mannose and fucose expression profiles.
33748076	8	38	gly	glycoproteins	1105:1117	arg1	glycoproteins					1105:1117	the GlcNAc or Galβ1-4GlcNA-containing glycoproteins	1067:1117	glycoproteins	1105:1117	Datura stramonium (DSA) was selected to isolate the GlcNAc or Galβ1-4GlcNA-containing glycoproteins due to the distinct difference between ESCC patients and HVs.
33748076	0	39	theme	Salivary	30:37	arg1	Glycoproteins					39:51	Salivary Glycoproteins	30:51	Salivary Glycoproteins	30:51	The Abnormal Glycopatterns of Salivary Glycoproteins in Esophageal Squamous Cell Carcinoma Patients.
33748076	11	40	theme	Gal	1560:1562	arg1	levels					1619:1624	lower levels	1613:1624	lower levels of mannose and fucose expression profiles	1613:1666	The ESCC patients showed higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles and lower levels of mannose and fucose expression profiles.
33748076	11	40	theme	Gal	1560:1562	arg1	levels					1539:1544	higher levels	1532:1544	higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles	1532:1607	The ESCC patients showed higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles and lower levels of mannose and fucose expression profiles.
33748076	10	41	dep	ECA	1428:1430	arg1	e.g.					1422:1425	e.g.	1422:1425	e.g.	1422:1425	As a result, the glycopatterns recognized by 13 lectins (e.g., ECA, RCA120, and DSA) showed significant alterations in ESCC patients' saliva.
33748076	8	42	theme	GlcNAc	1071:1076	arg1	glycoproteins					1105:1117	the GlcNAc or Galβ1-4GlcNA-containing glycoproteins	1067:1117	glycoproteins	1105:1117	Datura stramonium (DSA) was selected to isolate the GlcNAc or Galβ1-4GlcNA-containing glycoproteins due to the distinct difference between ESCC patients and HVs.
33748076	10	43	theme	ESCC	1484:1487	arg1	patients					1489:1496	ESCC patients'	1484:1497	ESCC patients' saliva	1484:1504	As a result, the glycopatterns recognized by 13 lectins (e.g., ECA, RCA120, and DSA) showed significant alterations in ESCC patients' saliva.
33748076	11	44	theme	GalNAc	1549:1554	arg1	levels					1619:1624	lower levels	1613:1624	lower levels of mannose and fucose expression profiles	1613:1666	The ESCC patients showed higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles and lower levels of mannose and fucose expression profiles.
33748076	11	44	theme	GalNAc	1549:1554	arg1	levels					1539:1544	higher levels	1532:1544	higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles	1532:1607	The ESCC patients showed higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles and lower levels of mannose and fucose expression profiles.
33748076	7	45	theme	glycomic	877:884	arg1	strategy					886:893	The glycomic strategy	873:893	The glycomic strategy combining lectin microarray and lectin blotting	873:941	The glycomic strategy combining lectin microarray and lectin blotting was employed to investigate and confirm the altered salivary glycopatterns.
33748076	11	46	dep	mannose	1629:1635	arg1	profiles					1659:1666	expression profiles	1648:1666	expression profiles	1648:1666	The ESCC patients showed higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles and lower levels of mannose and fucose expression profiles.
33748076	8	47	theme	Galβ1-4GlcNA-containing	1081:1103	arg1	glycoproteins					1105:1117	the GlcNAc or Galβ1-4GlcNA-containing glycoproteins	1067:1117	glycoproteins	1105:1117	Datura stramonium (DSA) was selected to isolate the GlcNAc or Galβ1-4GlcNA-containing glycoproteins due to the distinct difference between ESCC patients and HVs.
33748076	12	48	theme	Galβ1-4GlcNAc-containing	1745:1768	arg1	N-glycans					1770:1778	the GlcNAc or Galβ1-4GlcNAc-containing N-glycans	1731:1778	N-glycans	1770:1778	The MALDI-TOF/TOF-MS results indicated that the proportion of the GlcNAc or Galβ1-4GlcNAc-containing N-glycans was increased in ESCC patients (79.04%) compared with HV (63.20%), which was consistent with the results of lectin microarrays.
33748076	13	49	theme	comprehensive	1929:1941	arg1	information					1943:1953	comprehensive information	1929:1953	comprehensive information to understand the complex physiological changes in ESCC patients	1929:2018	Our findings provide comprehensive information to understand the complex physiological changes in ESCC patients.
33748076	8	50	theme	due	1119:1121	arg1	glycoproteins					1105:1117	the GlcNAc or Galβ1-4GlcNA-containing glycoproteins	1067:1117	glycoproteins	1105:1117	Datura stramonium (DSA) was selected to isolate the GlcNAc or Galβ1-4GlcNA-containing glycoproteins due to the distinct difference between ESCC patients and HVs.
33748076	14	51	theme	patients	2193:2200	arg1	diagnosis					2175:2183	the diagnosis	2171:2183	the diagnosis of ESCC patients	2171:2200	And the altered salivary glycopatterns such as GlcNAc or Galβ1-4GlcNAc-containing N-glycans recognized by DSA might serve as potential biomarkers for the diagnosis of ESCC patients.
33748076	11	52	theme	sialic	1565:1570	arg1	acid					1572:1575	sialic acid	1565:1575	sialic acid	1565:1575	The ESCC patients showed higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles and lower levels of mannose and fucose expression profiles.
33748076	4	53	theme	severe	516:521	arg1	threat					523:528	a severe threat	514:528	a severe threat to human health	514:544	Esophageal squamous cell carcinoma (ESCC) is the primary pathological histology in developing countries and a severe threat to human health.
33748076	4	54	theme	cell	426:429	arg1	histology					476:484	the primary pathological histology	451:484	the primary pathological histology in developing countries	451:508	Esophageal squamous cell carcinoma (ESCC) is the primary pathological histology in developing countries and a severe threat to human health.
33748076	4	54	theme	cell	426:429	arg1	ESCC					442:445	ESCC	442:445	ESCC	442:445	Esophageal squamous cell carcinoma (ESCC) is the primary pathological histology in developing countries and a severe threat to human health.
33748076	4	54	theme	cell	426:429	arg1	carcinoma					431:439	Esophageal squamous cell carcinoma	406:439	Esophageal squamous cell carcinoma (ESCC)	406:446	Esophageal squamous cell carcinoma (ESCC) is the primary pathological histology in developing countries and a severe threat to human health.
33748076	11	55	theme	GlcNAc	1582:1587	arg1	levels					1619:1624	lower levels	1613:1624	lower levels of mannose and fucose expression profiles	1613:1666	The ESCC patients showed higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles and lower levels of mannose and fucose expression profiles.
33748076	11	55	theme	GlcNAc	1582:1587	arg1	levels					1539:1544	higher levels	1532:1544	higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles	1532:1607	The ESCC patients showed higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles and lower levels of mannose and fucose expression profiles.
33748076	4	56	from	threat	523:528	arg1	countries					500:508	developing countries	489:508	developing countries	489:508	Esophageal squamous cell carcinoma (ESCC) is the primary pathological histology in developing countries and a severe threat to human health.
33748076	6	57	theme	=	815:815	arg1	n					813:813	n = 16	813:818	n = 16	813:818	In the present study, ESCC patients (n = 16) and healthy volunteers (HVs, n = 25) were enrolled.
33748076	6	57	theme	=	815:815	arg1	patients					803:810	ESCC patients	798:810	ESCC patients (n = 16)	798:819	In the present study, ESCC patients (n = 16) and healthy volunteers (HVs, n = 25) were enrolled.
33748076	12	58	theme	GlcNAc	1735:1740	arg1	N-glycans					1770:1778	the GlcNAc or Galβ1-4GlcNAc-containing N-glycans	1731:1778	N-glycans	1770:1778	The MALDI-TOF/TOF-MS results indicated that the proportion of the GlcNAc or Galβ1-4GlcNAc-containing N-glycans was increased in ESCC patients (79.04%) compared with HV (63.20%), which was consistent with the results of lectin microarrays.
33748076	5	59	theme	glycomic	634:641	arg1	methods					662:668	glycomic and glycoproteomic methods	634:668	glycomic and glycoproteomic methods	634:668	Although the glycan profiles in the blood samples of ESCC patients were analyzed using glycomic and glycoproteomic methods, the difference of salivary glycopatterns between healthy subjects and ESCC patients is not explicit yet.
33748076	2	60	theme	certain	304:310	arg1	diseases					312:319	certain diseases	304:319	certain diseases	304:319	The alteration of glycosylation is closely associated with certain diseases.
33748076	8	61	theme	ESCC	1158:1161	arg1	patients					1163:1170	ESCC patients	1158:1170	ESCC patients	1158:1170	Datura stramonium (DSA) was selected to isolate the GlcNAc or Galβ1-4GlcNA-containing glycoproteins due to the distinct difference between ESCC patients and HVs.
33748076	11	62	theme	lower	1613:1617	arg1	levels					1619:1624	lower levels	1613:1624	lower levels of mannose and fucose expression profiles	1613:1666	The ESCC patients showed higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles and lower levels of mannose and fucose expression profiles.
33748076	5	63	theme	glycoproteomic	647:660	arg1	methods					662:668	glycomic and glycoproteomic methods	634:668	glycomic and glycoproteomic methods	634:668	Although the glycan profiles in the blood samples of ESCC patients were analyzed using glycomic and glycoproteomic methods, the difference of salivary glycopatterns between healthy subjects and ESCC patients is not explicit yet.
33748076	6	64	theme	ESCC	798:801	arg1	n					813:813	n = 16	813:818	n = 16	813:818	In the present study, ESCC patients (n = 16) and healthy volunteers (HVs, n = 25) were enrolled.
33748076	6	64	theme	ESCC	798:801	arg1	patients					803:810	ESCC patients	798:810	ESCC patients (n = 16)	798:819	In the present study, ESCC patients (n = 16) and healthy volunteers (HVs, n = 25) were enrolled.
33748076	12	65	theme	microarrays	1895:1905	arg1	results					1877:1883	the results	1873:1883	the results of lectin microarrays	1873:1905	The MALDI-TOF/TOF-MS results indicated that the proportion of the GlcNAc or Galβ1-4GlcNAc-containing N-glycans was increased in ESCC patients (79.04%) compared with HV (63.20%), which was consistent with the results of lectin microarrays.
33748076	4	66	theme	Esophageal	406:415	arg1	histology					476:484	the primary pathological histology	451:484	the primary pathological histology in developing countries	451:508	Esophageal squamous cell carcinoma (ESCC) is the primary pathological histology in developing countries and a severe threat to human health.
33748076	4	66	theme	Esophageal	406:415	arg1	ESCC					442:445	ESCC	442:445	ESCC	442:445	Esophageal squamous cell carcinoma (ESCC) is the primary pathological histology in developing countries and a severe threat to human health.
33748076	4	66	theme	Esophageal	406:415	arg1	carcinoma					431:439	Esophageal squamous cell carcinoma	406:439	Esophageal squamous cell carcinoma (ESCC)	406:446	Esophageal squamous cell carcinoma (ESCC) is the primary pathological histology in developing countries and a severe threat to human health.
33748076	13	67	from	changes	1995:2001	arg1	patients					2011:2018	ESCC patients	2006:2018	ESCC patients	2006:2018	Our findings provide comprehensive information to understand the complex physiological changes in ESCC patients.
33748076	4	68	theme	pathological	463:474	arg1	carcinoma					431:439	Esophageal squamous cell carcinoma	406:439	Esophageal squamous cell carcinoma (ESCC)	406:446	Esophageal squamous cell carcinoma (ESCC) is the primary pathological histology in developing countries and a severe threat to human health.
33748076	4	68	theme	pathological	463:474	arg1	histology					476:484	the primary pathological histology	451:484	the primary pathological histology in developing countries	451:508	Esophageal squamous cell carcinoma (ESCC) is the primary pathological histology in developing countries and a severe threat to human health.
33748076	5	69	theme	ESCC	741:744	arg1	patients					746:753	ESCC patients	741:753	ESCC patients	741:753	Although the glycan profiles in the blood samples of ESCC patients were analyzed using glycomic and glycoproteomic methods, the difference of salivary glycopatterns between healthy subjects and ESCC patients is not explicit yet.
33748076	1	70	theme	biological	221:230	arg1	information					232:242	biological information	221:242	biological information	221:242	Glycosylation is one of the most crucial posttranslational modifications of proteins, containing a remarkable amount of biological information.
33748076	5	71	theme	salivary	689:696	arg1	glycopatterns					698:710	salivary glycopatterns	689:710	salivary glycopatterns	689:710	Although the glycan profiles in the blood samples of ESCC patients were analyzed using glycomic and glycoproteomic methods, the difference of salivary glycopatterns between healthy subjects and ESCC patients is not explicit yet.
33748076	9	72	theme	PNGase	1244:1249	arg1	F					1251:1251	PNGase F	1244:1251	PNGase F	1244:1251	The N-glycans from DSA-enriched glycoproteins were released by PNGase F and further identified by MALDI-TOF/TOF-MS to obtain the precise structural information of the altered glycans.
33748076	7	73	theme	salivary	995:1002	arg1	glycopatterns					1004:1016	the altered salivary glycopatterns	983:1016	the altered salivary glycopatterns	983:1016	The glycomic strategy combining lectin microarray and lectin blotting was employed to investigate and confirm the altered salivary glycopatterns.
33748076	6	74	theme	healthy	825:831	arg1	HVs					845:847	HVs	845:847	HVs	845:847	In the present study, ESCC patients (n = 16) and healthy volunteers (HVs, n = 25) were enrolled.
33748076	6	74	theme	healthy	825:831	arg1	volunteers					833:842	healthy volunteers	825:842	healthy volunteers (HVs, n = 25)	825:856	In the present study, ESCC patients (n = 16) and healthy volunteers (HVs, n = 25) were enrolled.
33748076	9	75	from	glycoproteins	1213:1225	arg1	N-glycans					1185:1193	The N-glycans	1181:1193	The N-glycans from DSA-enriched glycoproteins	1181:1225	The N-glycans from DSA-enriched glycoproteins were released by PNGase F and further identified by MALDI-TOF/TOF-MS to obtain the precise structural information of the altered glycans.
33748076	1	76	theme	most	129:132	arg1	modifications					160:172	the most crucial posttranslational modifications	125:172	the most crucial posttranslational modifications of proteins	125:184	Glycosylation is one of the most crucial posttranslational modifications of proteins, containing a remarkable amount of biological information.
33748076	13	77	theme	physiological	1981:1993	arg1	changes					1995:2001	the complex physiological changes	1969:2001	the complex physiological changes in ESCC patients	1969:2018	Our findings provide comprehensive information to understand the complex physiological changes in ESCC patients.
33748076	0	78	theme	Cell	76:79	arg1	Carcinoma					81:89	Esophageal Squamous Cell Carcinoma	56:89	Esophageal Squamous Cell Carcinoma Patients	56:98	The Abnormal Glycopatterns of Salivary Glycoproteins in Esophageal Squamous Cell Carcinoma Patients.
33748076	1	79	theme	posttranslational	142:158	arg1	modifications					160:172	the most crucial posttranslational modifications	125:172	the most crucial posttranslational modifications of proteins	125:184	Glycosylation is one of the most crucial posttranslational modifications of proteins, containing a remarkable amount of biological information.
33748076	10	80	from	alterations	1469:1479	arg1	saliva					1499:1504	ESCC patients' saliva	1484:1504	ESCC patients' saliva	1484:1504	As a result, the glycopatterns recognized by 13 lectins (e.g., ECA, RCA120, and DSA) showed significant alterations in ESCC patients' saliva.
33748076	4	81	theme	human	533:537	arg1	health					539:544	human health	533:544	human health	533:544	Esophageal squamous cell carcinoma (ESCC) is the primary pathological histology in developing countries and a severe threat to human health.
33748076	14	82	theme	Galβ1-4GlcNAc-containing	2078:2101	arg1	N-glycans					2103:2111	Galβ1-4GlcNAc-containing N-glycans	2078:2111	Galβ1-4GlcNAc-containing N-glycans	2078:2111	And the altered salivary glycopatterns such as GlcNAc or Galβ1-4GlcNAc-containing N-glycans recognized by DSA might serve as potential biomarkers for the diagnosis of ESCC patients.
33748076	9	83	theme	precise	1310:1316	arg1	information					1329:1339	the precise structural information	1306:1339	the precise structural information of the altered glycans	1306:1362	The N-glycans from DSA-enriched glycoproteins were released by PNGase F and further identified by MALDI-TOF/TOF-MS to obtain the precise structural information of the altered glycans.
33748076	7	84	theme	lectin	927:932	arg1	blotting					934:941	lectin blotting	927:941	lectin blotting	927:941	The glycomic strategy combining lectin microarray and lectin blotting was employed to investigate and confirm the altered salivary glycopatterns.
33748076	3	85	theme	hot	379:381	arg1	topic					383:387	a hot topic	377:387	a hot topic in recent years	377:403	Exploring glyco-code in the development of diseases is a hot topic in recent years.
33748076	0	86	theme	Abnormal	4:11	arg1	Glycopatterns					13:25	The Abnormal Glycopatterns	0:25	The Abnormal Glycopatterns of Salivary Glycoproteins in Esophageal Squamous Cell Carcinoma Patients	0:98	The Abnormal Glycopatterns of Salivary Glycoproteins in Esophageal Squamous Cell Carcinoma Patients.
33748076	14	87	theme	salivary	2037:2044	arg1	N-glycans					2103:2111	Galβ1-4GlcNAc-containing N-glycans	2078:2111	Galβ1-4GlcNAc-containing N-glycans	2078:2111	And the altered salivary glycopatterns such as GlcNAc or Galβ1-4GlcNAc-containing N-glycans recognized by DSA might serve as potential biomarkers for the diagnosis of ESCC patients.
33748076	14	87	theme	salivary	2037:2044	arg1	glycopatterns					2046:2058	the altered salivary glycopatterns	2025:2058	the altered salivary glycopatterns such as GlcNAc or Galβ1-4GlcNAc-containing N-glycans recognized by DSA	2025:2129	And the altered salivary glycopatterns such as GlcNAc or Galβ1-4GlcNAc-containing N-glycans recognized by DSA might serve as potential biomarkers for the diagnosis of ESCC patients.
33748076	14	87	theme	salivary	2037:2044	arg1	GlcNAc					2068:2073	GlcNAc	2068:2073	GlcNAc	2068:2073	And the altered salivary glycopatterns such as GlcNAc or Galβ1-4GlcNAc-containing N-glycans recognized by DSA might serve as potential biomarkers for the diagnosis of ESCC patients.
33748076	14	87	theme	salivary	2037:2044	arg1	biomarkers					2156:2165	potential biomarkers	2146:2165	potential biomarkers for the diagnosis of ESCC patients	2146:2200	And the altered salivary glycopatterns such as GlcNAc or Galβ1-4GlcNAc-containing N-glycans recognized by DSA might serve as potential biomarkers for the diagnosis of ESCC patients.
33748076	12	88	with	consistent	1857:1866	arg1	results					1877:1883	the results	1873:1883	the results of lectin microarrays	1873:1905	The MALDI-TOF/TOF-MS results indicated that the proportion of the GlcNAc or Galβ1-4GlcNAc-containing N-glycans was increased in ESCC patients (79.04%) compared with HV (63.20%), which was consistent with the results of lectin microarrays.
33748076	5	89	theme	ESCC	600:603	arg1	patients					605:612	ESCC patients	600:612	ESCC patients	600:612	Although the glycan profiles in the blood samples of ESCC patients were analyzed using glycomic and glycoproteomic methods, the difference of salivary glycopatterns between healthy subjects and ESCC patients is not explicit yet.
33748076	10	90	theme	significant	1457:1467	arg1	alterations					1469:1479	significant alterations	1457:1479	significant alterations in ESCC patients' saliva	1457:1504	As a result, the glycopatterns recognized by 13 lectins (e.g., ECA, RCA120, and DSA) showed significant alterations in ESCC patients' saliva.
33748076	10	90	theme	significant	1457:1467	arg1	result					1370:1375	a result	1368:1375	a result	1368:1375	As a result, the glycopatterns recognized by 13 lectins (e.g., ECA, RCA120, and DSA) showed significant alterations in ESCC patients' saliva.
33748076	11	91	theme	fucose	1641:1646	arg1	levels					1619:1624	lower levels	1613:1624	lower levels of mannose and fucose expression profiles	1613:1666	The ESCC patients showed higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles and lower levels of mannose and fucose expression profiles.
33748076	11	91	theme	fucose	1641:1646	arg1	levels					1539:1544	higher levels	1532:1544	higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles	1532:1607	The ESCC patients showed higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles and lower levels of mannose and fucose expression profiles.
33748076	0	92	theme	Glycoproteins	39:51	arg1	Glycopatterns					13:25	The Abnormal Glycopatterns	0:25	The Abnormal Glycopatterns of Salivary Glycoproteins in Esophageal Squamous Cell Carcinoma Patients	0:98	The Abnormal Glycopatterns of Salivary Glycoproteins in Esophageal Squamous Cell Carcinoma Patients.
33748076	9	93	theme	glycans	1356:1362	arg1	information					1329:1339	the precise structural information	1306:1339	the precise structural information of the altered glycans	1306:1362	The N-glycans from DSA-enriched glycoproteins were released by PNGase F and further identified by MALDI-TOF/TOF-MS to obtain the precise structural information of the altered glycans.
33748076	6	94	dep	HVs	845:847	arg1	n					850:850	n = 25	850:855	n = 25	850:855	In the present study, ESCC patients (n = 16) and healthy volunteers (HVs, n = 25) were enrolled.
33748076	11	95	theme	mannose	1629:1635	arg1	levels					1619:1624	lower levels	1613:1624	lower levels of mannose and fucose expression profiles	1613:1666	The ESCC patients showed higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles and lower levels of mannose and fucose expression profiles.
33748076	11	95	theme	mannose	1629:1635	arg1	levels					1539:1544	higher levels	1532:1544	higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles	1532:1607	The ESCC patients showed higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles and lower levels of mannose and fucose expression profiles.
33748076	6	96	theme	present	783:789	arg1	study					791:795	the present study	779:795	the present study	779:795	In the present study, ESCC patients (n = 16) and healthy volunteers (HVs, n = 25) were enrolled.
33748076	0	97	theme	Esophageal	56:65	arg1	Carcinoma					81:89	Esophageal Squamous Cell Carcinoma	56:89	Esophageal Squamous Cell Carcinoma Patients	56:98	The Abnormal Glycopatterns of Salivary Glycoproteins in Esophageal Squamous Cell Carcinoma Patients.
33748076	13	98	theme	ESCC	2006:2009	arg1	patients					2011:2018	ESCC patients	2006:2018	ESCC patients	2006:2018	Our findings provide comprehensive information to understand the complex physiological changes in ESCC patients.
33748076	13	99	theme	complex	1973:1979	arg1	changes					1995:2001	the complex physiological changes	1969:2001	the complex physiological changes in ESCC patients	1969:2018	Our findings provide comprehensive information to understand the complex physiological changes in ESCC patients.
33748076	7	100	theme	lectin	905:910	arg1	microarray					912:921	lectin microarray	905:921	lectin microarray	905:921	The glycomic strategy combining lectin microarray and lectin blotting was employed to investigate and confirm the altered salivary glycopatterns.
33748076	12	101	theme	ESCC	1797:1800	arg1	%					1817:1817	79.04%	1812:1817	79.04%	1812:1817	The MALDI-TOF/TOF-MS results indicated that the proportion of the GlcNAc or Galβ1-4GlcNAc-containing N-glycans was increased in ESCC patients (79.04%) compared with HV (63.20%), which was consistent with the results of lectin microarrays.
33748076	12	101	theme	ESCC	1797:1800	arg1	patients					1802:1809	ESCC patients	1797:1809	ESCC patients (79.04%)	1797:1818	The MALDI-TOF/TOF-MS results indicated that the proportion of the GlcNAc or Galβ1-4GlcNAc-containing N-glycans was increased in ESCC patients (79.04%) compared with HV (63.20%), which was consistent with the results of lectin microarrays.
33748076	5	102	theme	glycan	560:565	arg1	profiles					567:574	the glycan profiles	556:574	the glycan profiles in the blood samples of ESCC patients	556:612	Although the glycan profiles in the blood samples of ESCC patients were analyzed using glycomic and glycoproteomic methods, the difference of salivary glycopatterns between healthy subjects and ESCC patients is not explicit yet.
33748076	11	103	theme	acid	1572:1575	arg1	levels					1619:1624	lower levels	1613:1624	lower levels of mannose and fucose expression profiles	1613:1666	The ESCC patients showed higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles and lower levels of mannose and fucose expression profiles.
33748076	11	103	theme	acid	1572:1575	arg1	levels					1539:1544	higher levels	1532:1544	higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles	1532:1607	The ESCC patients showed higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles and lower levels of mannose and fucose expression profiles.
33748076	5	104	from	profiles	567:574	arg1	samples					589:595	the blood samples	579:595	the blood samples of ESCC patients	579:612	Although the glycan profiles in the blood samples of ESCC patients were analyzed using glycomic and glycoproteomic methods, the difference of salivary glycopatterns between healthy subjects and ESCC patients is not explicit yet.
33748076	12	105	theme	lectin	1888:1893	arg1	microarrays					1895:1905	lectin microarrays	1888:1905	lectin microarrays	1888:1905	The MALDI-TOF/TOF-MS results indicated that the proportion of the GlcNAc or Galβ1-4GlcNAc-containing N-glycans was increased in ESCC patients (79.04%) compared with HV (63.20%), which was consistent with the results of lectin microarrays.
33748076	11	106	theme	expression	1589:1598	arg1	profiles					1600:1607	expression profiles	1589:1607	expression profiles	1589:1607	The ESCC patients showed higher levels of GalNAc and Gal, sialic acid, and GlcNAc expression profiles and lower levels of mannose and fucose expression profiles.
33848546	6	0	theme	Hemolysis	747:755	arg1	tests					757:761	Hemolysis tests	747:761	Hemolysis tests	747:761	Hemolysis tests indicated that the prepared CAHSs were non-hemolytic material (hemolysis ratio < 5%, no cytotoxicity).
33848546	2	1	theme	mechanical	406:415	arg1	properties					417:426	mechanical properties	406:426	mechanical properties of materials	406:439	Genipin as excellent natural biological crosslinker was chose for high biocompatibility and improving mechanical properties of materials.
33848546	0	2	theme	dressing	103:110	arg1	application					112:122	wound dressing application	97:122	wound dressing application	97:122	Chitosan/alginate/hyaluronic acid polyelectrolyte composite sponges crosslinked with genipin for wound dressing application.
33848546	5	3	theme	cytotoxicity	630:641	arg1	assay					643:647	The cytotoxicity assay	626:647	The cytotoxicity assay	626:647	The cytotoxicity assay was carried out on HUVEC cells in vitro and the result proves the good biocompatibility of CAHSs.
33848546	7	4	theme	coagulation	878:888	arg1	tests					890:894	PT and aPPT coagulation tests	866:894	PT and aPPT coagulation tests	866:894	PT and aPPT coagulation tests demonstrated that CAHS2 and CAHS3 could both activate the extrinsic and intrinsic coagulation pathway and thus accelerated blood coagulation.
33848546	1	5	theme	freeze-dried	282:293	arg1	molding					295:301	freeze-dried molding	282:301	freeze-dried molding	282:301	Wound dressing composed of polyelectrolyte complexes (PECs), based on chitosan/alginate/hyaluronic acid (CS/ALG/HYA) crosslinked by genipin, was prepared by freeze-dried molding.
33848546	8	6	theme	full-thickness	1056:1069	arg1	model					1078:1082	a rat full-thickness wounds model	1050:1082	a rat full-thickness wounds model	1050:1082	Further, in a rat full-thickness wounds model, the CAHS2 sponge significantly facilitates wound closure compared to other groups.
33848546	9	7	theme	biological	1220:1229	arg1	properties					1231:1240	adjustable physical, mechanical and biological properties	1184:1240	adjustable physical, mechanical and biological properties	1184:1240	CAHSs exhibited adjustable physical, mechanical and biological properties.
33848546	2	8	theme	natural	325:331	arg1	crosslinker					344:354	excellent natural biological crosslinker	315:354	excellent natural biological crosslinker	315:354	Genipin as excellent natural biological crosslinker was chose for high biocompatibility and improving mechanical properties of materials.
33848546	8	9	theme	rat	1052:1054	arg1	model					1078:1082	a rat full-thickness wounds model	1050:1082	a rat full-thickness wounds model	1050:1082	Further, in a rat full-thickness wounds model, the CAHS2 sponge significantly facilitates wound closure compared to other groups.
33848546	6	10	theme	<	842:842	arg1	%					845:845	hemolysis ratio < 5%	826:845	hemolysis ratio < 5%	826:845	Hemolysis tests indicated that the prepared CAHSs were non-hemolytic material (hemolysis ratio < 5%, no cytotoxicity).
33848546	2	11	theme	excellent	315:323	arg1	crosslinker					344:354	excellent natural biological crosslinker	315:354	excellent natural biological crosslinker	315:354	Genipin as excellent natural biological crosslinker was chose for high biocompatibility and improving mechanical properties of materials.
33848546	10	12	theme	promising	1329:1337	arg1	dressings					1345:1353	promising wound dressings	1329:1353	promising wound dressings	1329:1353	Thus, the chitosan-based polyelectrolyte composite sponges exhibit great potential as promising wound dressings.
33848546	10	12	theme	promising	1329:1337	arg1	potential					1316:1324	great potential	1310:1324	great potential	1310:1324	Thus, the chitosan-based polyelectrolyte composite sponges exhibit great potential as promising wound dressings.
33848546	6	13	theme	ratio	836:840	arg1	%					845:845	hemolysis ratio < 5%	826:845	hemolysis ratio < 5%	826:845	Hemolysis tests indicated that the prepared CAHSs were non-hemolytic material (hemolysis ratio < 5%, no cytotoxicity).
33848546	7	14	theme	aPPT	873:876	arg1	tests					890:894	PT and aPPT coagulation tests	866:894	PT and aPPT coagulation tests	866:894	PT and aPPT coagulation tests demonstrated that CAHS2 and CAHS3 could both activate the extrinsic and intrinsic coagulation pathway and thus accelerated blood coagulation.
33848546	9	15	theme	mechanical	1205:1214	arg1	properties					1231:1240	adjustable physical, mechanical and biological properties	1184:1240	adjustable physical, mechanical and biological properties	1184:1240	CAHSs exhibited adjustable physical, mechanical and biological properties.
33848546	8	16	theme	wounds	1071:1076	arg1	model					1078:1082	a rat full-thickness wounds model	1050:1082	a rat full-thickness wounds model	1050:1082	Further, in a rat full-thickness wounds model, the CAHS2 sponge significantly facilitates wound closure compared to other groups.
33848546	6	17	theme	hemolysis	826:834	arg1	%					845:845	hemolysis ratio < 5%	826:845	hemolysis ratio < 5%	826:845	Hemolysis tests indicated that the prepared CAHSs were non-hemolytic material (hemolysis ratio < 5%, no cytotoxicity).
33848546	0	18	theme	Chitosan/alginate/hyaluronic	0:27	arg1	acid					29:32	Chitosan/alginate/hyaluronic acid	0:32	Chitosan/alginate/hyaluronic acid	0:32	Chitosan/alginate/hyaluronic acid polyelectrolyte composite sponges crosslinked with genipin for wound dressing application.
33848546	10	19	theme	wound	1339:1343	arg1	dressings					1345:1353	promising wound dressings	1329:1353	promising wound dressings	1329:1353	Thus, the chitosan-based polyelectrolyte composite sponges exhibit great potential as promising wound dressings.
33848546	10	19	theme	wound	1339:1343	arg1	potential					1316:1324	great potential	1310:1324	great potential	1310:1324	Thus, the chitosan-based polyelectrolyte composite sponges exhibit great potential as promising wound dressings.
33848546	1	20	theme	chitosan/alginate/hyaluronic	195:222	arg1	acid					224:227	chitosan/alginate/hyaluronic acid	195:227	chitosan/alginate/hyaluronic acid (CS/ALG/HYA) crosslinked by genipin	195:263	Wound dressing composed of polyelectrolyte complexes (PECs), based on chitosan/alginate/hyaluronic acid (CS/ALG/HYA) crosslinked by genipin, was prepared by freeze-dried molding.
33848546	1	20	theme	chitosan/alginate/hyaluronic	195:222	arg1	CS/ALG/HYA					230:239	CS/ALG/HYA	230:239	CS/ALG/HYA	230:239	Wound dressing composed of polyelectrolyte complexes (PECs), based on chitosan/alginate/hyaluronic acid (CS/ALG/HYA) crosslinked by genipin, was prepared by freeze-dried molding.
33848546	5	21	theme	HUVEC	668:672	arg1	cells					674:678	HUVEC cells	668:678	HUVEC cells	668:678	The cytotoxicity assay was carried out on HUVEC cells in vitro and the result proves the good biocompatibility of CAHSs.
33848546	7	22	theme	extrinsic	954:962	arg1	pathway					990:996	the extrinsic and intrinsic coagulation pathway	950:996	the extrinsic and intrinsic coagulation pathway	950:996	PT and aPPT coagulation tests demonstrated that CAHS2 and CAHS3 could both activate the extrinsic and intrinsic coagulation pathway and thus accelerated blood coagulation.
33848546	7	23	theme	PT	866:867	arg1	tests					890:894	PT and aPPT coagulation tests	866:894	PT and aPPT coagulation tests	866:894	PT and aPPT coagulation tests demonstrated that CAHS2 and CAHS3 could both activate the extrinsic and intrinsic coagulation pathway and thus accelerated blood coagulation.
33848546	3	24	theme	CS/ALG/HYA	446:455	arg1	CAHSs					466:470	CAHSs	466:470	CAHSs	466:470	The CS/ALG/HYA sponges (CAHSs) were characterized by FTIR, XRD, DSC and SEM.
33848546	3	24	theme	CS/ALG/HYA	446:455	arg1	sponges					457:463	The CS/ALG/HYA sponges	442:463	The CS/ALG/HYA sponges (CAHSs)	442:471	The CS/ALG/HYA sponges (CAHSs) were characterized by FTIR, XRD, DSC and SEM.
33848546	8	25	theme	wound	1128:1132	arg1	closure					1134:1140	wound closure	1128:1140	wound closure	1128:1140	Further, in a rat full-thickness wounds model, the CAHS2 sponge significantly facilitates wound closure compared to other groups.
33848546	1	26	theme	Wound	125:129	arg1	dressing					131:138	Wound dressing	125:138	Wound dressing composed of polyelectrolyte complexes (PECs), based on chitosan/alginate/hyaluronic acid (CS/ALG/HYA) crosslinked by genipin,	125:264	Wound dressing composed of polyelectrolyte complexes (PECs), based on chitosan/alginate/hyaluronic acid (CS/ALG/HYA) crosslinked by genipin, was prepared by freeze-dried molding.
33848546	2	27	theme	materials	431:439	arg1	properties					417:426	mechanical properties	406:426	mechanical properties of materials	406:439	Genipin as excellent natural biological crosslinker was chose for high biocompatibility and improving mechanical properties of materials.
33848546	0	28	theme	composite	50:58	arg1	sponges					60:66	composite sponges	50:66	composite sponges crosslinked with genipin for wound dressing application	50:122	Chitosan/alginate/hyaluronic acid polyelectrolyte composite sponges crosslinked with genipin for wound dressing application.
33848546	6	29	theme	non-hemolytic	802:814	arg1	CAHSs					791:795	the prepared CAHSs	778:795	the prepared CAHSs	778:795	Hemolysis tests indicated that the prepared CAHSs were non-hemolytic material (hemolysis ratio < 5%, no cytotoxicity).
33848546	6	29	theme	non-hemolytic	802:814	arg1	material					816:823	non-hemolytic material	802:823	non-hemolytic material	802:823	Hemolysis tests indicated that the prepared CAHSs were non-hemolytic material (hemolysis ratio < 5%, no cytotoxicity).
33848546	7	30	theme	coagulation	978:988	arg1	pathway					990:996	the extrinsic and intrinsic coagulation pathway	950:996	the extrinsic and intrinsic coagulation pathway	950:996	PT and aPPT coagulation tests demonstrated that CAHS2 and CAHS3 could both activate the extrinsic and intrinsic coagulation pathway and thus accelerated blood coagulation.
33848546	9	31	theme	adjustable	1184:1193	arg1	properties					1231:1240	adjustable physical, mechanical and biological properties	1184:1240	adjustable physical, mechanical and biological properties	1184:1240	CAHSs exhibited adjustable physical, mechanical and biological properties.
33848546	4	32	theme	CAHSs	601:605	arg1	degradation					586:596	in vitro degradation	577:596	in vitro degradation	577:596	Porosity, swelling behavior and mechanical properties and in vitro degradation of CAHSs were investigated.
33848546	4	32	theme	CAHSs	601:605	arg1	Porosity					519:526	Porosity	519:526	Porosity	519:526	Porosity, swelling behavior and mechanical properties and in vitro degradation of CAHSs were investigated.
33848546	4	32	theme	CAHSs	601:605	arg1	behavior					538:545	swelling behavior	529:545	swelling behavior	529:545	Porosity, swelling behavior and mechanical properties and in vitro degradation of CAHSs were investigated.
33848546	4	32	theme	CAHSs	601:605	arg1	properties					562:571	mechanical properties	551:571	mechanical properties	551:571	Porosity, swelling behavior and mechanical properties and in vitro degradation of CAHSs were investigated.
33848546	8	33	theme	CAHS2	1089:1093	arg1	sponge					1095:1100	the CAHS2 sponge	1085:1100	the CAHS2 sponge	1085:1100	Further, in a rat full-thickness wounds model, the CAHS2 sponge significantly facilitates wound closure compared to other groups.
33848546	2	34	theme	biological	333:342	arg1	crosslinker					344:354	excellent natural biological crosslinker	315:354	excellent natural biological crosslinker	315:354	Genipin as excellent natural biological crosslinker was chose for high biocompatibility and improving mechanical properties of materials.
33848546	7	35	theme	intrinsic	968:976	arg1	pathway					990:996	the extrinsic and intrinsic coagulation pathway	950:996	the extrinsic and intrinsic coagulation pathway	950:996	PT and aPPT coagulation tests demonstrated that CAHS2 and CAHS3 could both activate the extrinsic and intrinsic coagulation pathway and thus accelerated blood coagulation.
33848546	6	36	dep	cytotoxicity	851:862	arg1	%					845:845	hemolysis ratio < 5%	826:845	hemolysis ratio < 5%	826:845	Hemolysis tests indicated that the prepared CAHSs were non-hemolytic material (hemolysis ratio < 5%, no cytotoxicity).
33848546	5	37	theme	CAHSs	740:744	arg1	biocompatibility					720:735	the good biocompatibility	711:735	the good biocompatibility of CAHSs	711:744	The cytotoxicity assay was carried out on HUVEC cells in vitro and the result proves the good biocompatibility of CAHSs.
33848546	10	38	theme	great	1310:1314	arg1	dressings					1345:1353	promising wound dressings	1329:1353	promising wound dressings	1329:1353	Thus, the chitosan-based polyelectrolyte composite sponges exhibit great potential as promising wound dressings.
33848546	10	38	theme	great	1310:1314	arg1	potential					1316:1324	great potential	1310:1324	great potential	1310:1324	Thus, the chitosan-based polyelectrolyte composite sponges exhibit great potential as promising wound dressings.
33848546	4	39	theme	mechanical	551:560	arg1	properties					562:571	mechanical properties	551:571	mechanical properties	551:571	Porosity, swelling behavior and mechanical properties and in vitro degradation of CAHSs were investigated.
33848546	4	40	theme	in	577:578	arg1	degradation					586:596	in vitro degradation	577:596	in vitro degradation	577:596	Porosity, swelling behavior and mechanical properties and in vitro degradation of CAHSs were investigated.
33848546	4	41	dep	in	577:578	arg1	vitro					580:584	vitro	580:584	vitro	580:584	Porosity, swelling behavior and mechanical properties and in vitro degradation of CAHSs were investigated.
33848546	6	42	theme	prepared	782:789	arg1	CAHSs					791:795	the prepared CAHSs	778:795	the prepared CAHSs	778:795	Hemolysis tests indicated that the prepared CAHSs were non-hemolytic material (hemolysis ratio < 5%, no cytotoxicity).
33848546	6	42	theme	prepared	782:789	arg1	material					816:823	non-hemolytic material	802:823	non-hemolytic material	802:823	Hemolysis tests indicated that the prepared CAHSs were non-hemolytic material (hemolysis ratio < 5%, no cytotoxicity).
33848546	1	43	theme	polyelectrolyte	152:166	arg1	complexes					168:176	polyelectrolyte complexes	152:176	polyelectrolyte complexes (PECs)	152:183	Wound dressing composed of polyelectrolyte complexes (PECs), based on chitosan/alginate/hyaluronic acid (CS/ALG/HYA) crosslinked by genipin, was prepared by freeze-dried molding.
33848546	1	43	theme	polyelectrolyte	152:166	arg1	PECs					179:182	PECs	179:182	PECs	179:182	Wound dressing composed of polyelectrolyte complexes (PECs), based on chitosan/alginate/hyaluronic acid (CS/ALG/HYA) crosslinked by genipin, was prepared by freeze-dried molding.
33848546	2	44	theme	high	370:373	arg1	biocompatibility					375:390	high biocompatibility	370:390	high biocompatibility	370:390	Genipin as excellent natural biological crosslinker was chose for high biocompatibility and improving mechanical properties of materials.
33848546	10	45	theme	composite	1284:1292	arg1	sponges					1294:1300	the chitosan-based polyelectrolyte composite sponges	1249:1300	the chitosan-based polyelectrolyte composite sponges	1249:1300	Thus, the chitosan-based polyelectrolyte composite sponges exhibit great potential as promising wound dressings.
33848546	5	46	theme	good	715:718	arg1	biocompatibility					720:735	the good biocompatibility	711:735	the good biocompatibility of CAHSs	711:744	The cytotoxicity assay was carried out on HUVEC cells in vitro and the result proves the good biocompatibility of CAHSs.
33848546	7	47	theme	accelerated	1007:1017	arg1	coagulation					1025:1035	accelerated blood coagulation	1007:1035	accelerated blood coagulation	1007:1035	PT and aPPT coagulation tests demonstrated that CAHS2 and CAHS3 could both activate the extrinsic and intrinsic coagulation pathway and thus accelerated blood coagulation.
33848546	10	48	theme	polyelectrolyte	1268:1282	arg1	sponges					1294:1300	the chitosan-based polyelectrolyte composite sponges	1249:1300	the chitosan-based polyelectrolyte composite sponges	1249:1300	Thus, the chitosan-based polyelectrolyte composite sponges exhibit great potential as promising wound dressings.
33848546	0	49	theme	wound	97:101	arg1	application					112:122	wound dressing application	97:122	wound dressing application	97:122	Chitosan/alginate/hyaluronic acid polyelectrolyte composite sponges crosslinked with genipin for wound dressing application.
33848546	7	50	theme	blood	1019:1023	arg1	coagulation					1025:1035	accelerated blood coagulation	1007:1035	accelerated blood coagulation	1007:1035	PT and aPPT coagulation tests demonstrated that CAHS2 and CAHS3 could both activate the extrinsic and intrinsic coagulation pathway and thus accelerated blood coagulation.
33848546	10	51	theme	chitosan-based	1253:1266	arg1	sponges					1294:1300	the chitosan-based polyelectrolyte composite sponges	1249:1300	the chitosan-based polyelectrolyte composite sponges	1249:1300	Thus, the chitosan-based polyelectrolyte composite sponges exhibit great potential as promising wound dressings.
33848546	8	52	theme	other	1154:1158	arg1	groups					1160:1165	other groups	1154:1165	other groups	1154:1165	Further, in a rat full-thickness wounds model, the CAHS2 sponge significantly facilitates wound closure compared to other groups.
33848546	4	53	theme	swelling	529:536	arg1	behavior					538:545	swelling behavior	529:545	swelling behavior	529:545	Porosity, swelling behavior and mechanical properties and in vitro degradation of CAHSs were investigated.
33848546	9	54	theme	physical	1195:1202	arg1	properties					1231:1240	adjustable physical, mechanical and biological properties	1184:1240	adjustable physical, mechanical and biological properties	1184:1240	CAHSs exhibited adjustable physical, mechanical and biological properties.
34364565	0	0	theme	blend	95:99	arg1	films					110:114	blend chitosan films	95:114	blend chitosan films	95:114	Understanding the effects of copolymerized cellulose nanofibers and diatomite nanocomposite on blend chitosan films.
34364565	0	1	from	effects	18:24	arg1	films					110:114	blend chitosan films	95:114	blend chitosan films	95:114	Understanding the effects of copolymerized cellulose nanofibers and diatomite nanocomposite on blend chitosan films.
34364565	5	2	theme	antioxidant	775:785	arg1	activity					787:794	The antioxidant activity	771:794	The antioxidant activity	771:794	The antioxidant activity gradually increased with an increasing concentration (1-5% and 10-30%) of copolymerized CNFs/diatomite composite in the chitosan matrix.
34364565	6	3	contain	containing	1036:1045	arg1	films					1030:1034	films	1030:1034	films containing 30% diatomite and 5% CNFs (CNFs-D30-5)	1030:1084	Moreover, the water solubility decreased from 30% for chitosan control film (CH-0) to 21.06% for films containing 30% diatomite and 5% CNFs (CNFs-D30-5).
34364565	6	3	contain	containing	1036:1045	arg2	diatomite					1051:1059	30% diatomite	1047:1059	30% diatomite	1047:1059	Moreover, the water solubility decreased from 30% for chitosan control film (CH-0) to 21.06% for films containing 30% diatomite and 5% CNFs (CNFs-D30-5).
34364565	6	3	contain	containing	1036:1045	arg2	CNFs-D30-5					1074:1083	CNFs-D30-5	1074:1083	CNFs-D30-5	1074:1083	Moreover, the water solubility decreased from 30% for chitosan control film (CH-0) to 21.06% for films containing 30% diatomite and 5% CNFs (CNFs-D30-5).
34364565	6	3	contain	containing	1036:1045	arg2	CNFs					1068:1071	5% CNFs	1065:1071	5% CNFs	1065:1071	Moreover, the water solubility decreased from 30% for chitosan control film (CH-0) to 21.06% for films containing 30% diatomite and 5% CNFs (CNFs-D30-5).
34364565	6	4	theme	chitosan	987:994	arg1	film					1004:1007	chitosan control film	987:1007	chitosan control film (CH-0)	987:1014	Moreover, the water solubility decreased from 30% for chitosan control film (CH-0) to 21.06% for films containing 30% diatomite and 5% CNFs (CNFs-D30-5).
34364565	6	4	theme	chitosan	987:994	arg1	CH-0					1010:1013	CH-0	1010:1013	CH-0	1010:1013	Moreover, the water solubility decreased from 30% for chitosan control film (CH-0) to 21.06% for films containing 30% diatomite and 5% CNFs (CNFs-D30-5).
34364565	7	5	from	distribution	1147:1158	arg1	matrix					1218:1223	the chitosan matrix	1205:1223	the chitosan matrix	1205:1223	The scanning electron micrographs showed an overall uniform distribution of copolymerized CNFs/diatomite composite in the chitosan matrix with punctual agglomerations.
34364565	5	6	theme	chitosan	916:923	arg1	matrix					925:930	the chitosan matrix	912:930	the chitosan matrix	912:930	The antioxidant activity gradually increased with an increasing concentration (1-5% and 10-30%) of copolymerized CNFs/diatomite composite in the chitosan matrix.
34364565	7	7	theme	CNFs/diatomite	1177:1190	arg1	composite					1192:1200	copolymerized CNFs/diatomite composite	1163:1200	copolymerized CNFs/diatomite composite	1163:1200	The scanning electron micrographs showed an overall uniform distribution of copolymerized CNFs/diatomite composite in the chitosan matrix with punctual agglomerations.
34364565	4	8	theme	CNFs/diatomite	671:684	arg1	composite					686:694	the copolymerized CNFs/diatomite composite	653:694	the copolymerized CNFs/diatomite composite	653:694	The mechanical characteristics and hydrophobicity of the films were observed to be improved after incorporating the copolymerized CNFs/diatomite composite at different concentrations (CNFs: 1%, 2% and 5%; diatomite: 10% and 30%).
34364565	0	9	theme	chitosan	101:108	arg1	films					110:114	blend chitosan films	95:114	blend chitosan films	95:114	Understanding the effects of copolymerized cellulose nanofibers and diatomite nanocomposite on blend chitosan films.
34364565	5	10	dep	concentration	835:847	arg1	%					853:853	1-5%	850:853	1-5%	850:853	The antioxidant activity gradually increased with an increasing concentration (1-5% and 10-30%) of copolymerized CNFs/diatomite composite in the chitosan matrix.
34364565	5	10	dep	concentration	835:847	arg1	%					864:864	10-30%	859:864	10-30%	859:864	The antioxidant activity gradually increased with an increasing concentration (1-5% and 10-30%) of copolymerized CNFs/diatomite composite in the chitosan matrix.
34364565	7	11	theme	punctual	1230:1237	arg1	agglomerations					1239:1252	punctual agglomerations	1230:1252	punctual agglomerations	1230:1252	The scanning electron micrographs showed an overall uniform distribution of copolymerized CNFs/diatomite composite in the chitosan matrix with punctual agglomerations.
34364565	6	12	theme	%	1066:1066	arg1	CNFs					1068:1071	5% CNFs	1065:1071	5% CNFs	1065:1071	Moreover, the water solubility decreased from 30% for chitosan control film (CH-0) to 21.06% for films containing 30% diatomite and 5% CNFs (CNFs-D30-5).
34364565	2	13	theme	polyacrylonitrile	341:357	arg1	monomers					359:366	polyacrylonitrile monomers	341:366	polyacrylonitrile monomers	341:366	Herein, we have produced chitosan composite films supplemented with copolymerized (with polyacrylonitrile monomers) cellulose nanofibers and diatomite nanocomposite at different concentrations.
34364565	2	14	theme	cellulose	369:377	arg1	nanofibers					379:388	cellulose nanofibers	369:388	cellulose nanofibers	369:388	Herein, we have produced chitosan composite films supplemented with copolymerized (with polyacrylonitrile monomers) cellulose nanofibers and diatomite nanocomposite at different concentrations.
34364565	2	15	theme	composite	287:295	arg1	films					297:301	chitosan composite films	278:301	chitosan composite films supplemented with copolymerized (with polyacrylonitrile monomers) cellulose nanofibers and diatomite nanocomposite at different concentrations	278:444	Herein, we have produced chitosan composite films supplemented with copolymerized (with polyacrylonitrile monomers) cellulose nanofibers and diatomite nanocomposite at different concentrations.
34364565	5	16	theme	copolymerized	870:882	arg1	composite					899:907	copolymerized CNFs/diatomite composite	870:907	copolymerized CNFs/diatomite composite	870:907	The antioxidant activity gradually increased with an increasing concentration (1-5% and 10-30%) of copolymerized CNFs/diatomite composite in the chitosan matrix.
34364565	4	17	theme	copolymerized	657:669	arg1	composite					686:694	the copolymerized CNFs/diatomite composite	653:694	the copolymerized CNFs/diatomite composite	653:694	The mechanical characteristics and hydrophobicity of the films were observed to be improved after incorporating the copolymerized CNFs/diatomite composite at different concentrations (CNFs: 1%, 2% and 5%; diatomite: 10% and 30%).
34364565	4	18	theme	mechanical	545:554	arg1	characteristics					556:570	mechanical characteristics	545:570	mechanical characteristics	545:570	The mechanical characteristics and hydrophobicity of the films were observed to be improved after incorporating the copolymerized CNFs/diatomite composite at different concentrations (CNFs: 1%, 2% and 5%; diatomite: 10% and 30%).
34364565	2	19	theme	chitosan	278:285	arg1	films					297:301	chitosan composite films	278:301	chitosan composite films supplemented with copolymerized (with polyacrylonitrile monomers) cellulose nanofibers and diatomite nanocomposite at different concentrations	278:444	Herein, we have produced chitosan composite films supplemented with copolymerized (with polyacrylonitrile monomers) cellulose nanofibers and diatomite nanocomposite at different concentrations.
34364565	6	20	theme	5	1065:1065	arg1	%					1066:1066	%	1066:1066	%	1066:1066	Moreover, the water solubility decreased from 30% for chitosan control film (CH-0) to 21.06% for films containing 30% diatomite and 5% CNFs (CNFs-D30-5).
34364565	5	21	theme	increasing	824:833	arg1	concentration					835:847	an increasing concentration	821:847	an increasing concentration (1-5% and 10-30%) of copolymerized CNFs/diatomite composite in the chitosan matrix	821:930	The antioxidant activity gradually increased with an increasing concentration (1-5% and 10-30%) of copolymerized CNFs/diatomite composite in the chitosan matrix.
34364565	5	22	from	concentration	835:847	arg1	matrix					925:930	the chitosan matrix	912:930	the chitosan matrix	912:930	The antioxidant activity gradually increased with an increasing concentration (1-5% and 10-30%) of copolymerized CNFs/diatomite composite in the chitosan matrix.
34364565	7	23	theme	chitosan	1209:1216	arg1	matrix					1218:1223	the chitosan matrix	1205:1223	the chitosan matrix	1205:1223	The scanning electron micrographs showed an overall uniform distribution of copolymerized CNFs/diatomite composite in the chitosan matrix with punctual agglomerations.
34364565	7	24	theme	uniform	1139:1145	arg1	distribution					1147:1158	an overall uniform distribution	1128:1158	an overall uniform distribution of copolymerized CNFs/diatomite composite in the chitosan matrix	1128:1223	The scanning electron micrographs showed an overall uniform distribution of copolymerized CNFs/diatomite composite in the chitosan matrix with punctual agglomerations.
34364565	2	25	theme	different	421:429	arg1	concentrations					431:444	different concentrations	421:444	different concentrations	421:444	Herein, we have produced chitosan composite films supplemented with copolymerized (with polyacrylonitrile monomers) cellulose nanofibers and diatomite nanocomposite at different concentrations.
34364565	3	26	theme	diatomite	477:485	arg1	incorporation					451:463	The incorporation	447:463	The incorporation of CNFs and diatomite	447:485	The incorporation of CNFs and diatomite enhanced the physicochemical properties of the films.
34364565	1	27	theme	reinforcing	215:225	arg1	agents					227:232	reinforcing agents	215:232	reinforcing agents	215:232	Chitosan films lack various important physicochemical properties and need to be supplemented with reinforcing agents to bridge the gap.
34364565	7	28	theme	overall	1131:1137	arg1	distribution					1147:1158	an overall uniform distribution	1128:1158	an overall uniform distribution of copolymerized CNFs/diatomite composite in the chitosan matrix	1128:1223	The scanning electron micrographs showed an overall uniform distribution of copolymerized CNFs/diatomite composite in the chitosan matrix with punctual agglomerations.
34364565	1	29	theme	Chitosan	117:124	arg1	films					126:130	Chitosan films	117:130	Chitosan films	117:130	Chitosan films lack various important physicochemical properties and need to be supplemented with reinforcing agents to bridge the gap.
34364565	5	30	theme	composite	899:907	arg1	concentration					835:847	an increasing concentration	821:847	an increasing concentration (1-5% and 10-30%) of copolymerized CNFs/diatomite composite in the chitosan matrix	821:930	The antioxidant activity gradually increased with an increasing concentration (1-5% and 10-30%) of copolymerized CNFs/diatomite composite in the chitosan matrix.
34364565	0	31	theme	cellulose	43:51	arg1	nanofibers					53:62	copolymerized cellulose nanofibers	29:62	copolymerized cellulose nanofibers	29:62	Understanding the effects of copolymerized cellulose nanofibers and diatomite nanocomposite on blend chitosan films.
34364565	5	32	theme	CNFs/diatomite	884:897	arg1	composite					899:907	copolymerized CNFs/diatomite composite	870:907	copolymerized CNFs/diatomite composite	870:907	The antioxidant activity gradually increased with an increasing concentration (1-5% and 10-30%) of copolymerized CNFs/diatomite composite in the chitosan matrix.
34364565	3	33	theme	physicochemical	500:514	arg1	properties					516:525	the physicochemical properties	496:525	the physicochemical properties of the films	496:538	The incorporation of CNFs and diatomite enhanced the physicochemical properties of the films.
34364565	3	34	theme	CNFs	468:471	arg1	incorporation					451:463	The incorporation	447:463	The incorporation of CNFs and diatomite	447:485	The incorporation of CNFs and diatomite enhanced the physicochemical properties of the films.
34364565	4	35	theme	films	598:602	arg1	hydrophobicity					576:589	hydrophobicity	576:589	hydrophobicity	576:589	The mechanical characteristics and hydrophobicity of the films were observed to be improved after incorporating the copolymerized CNFs/diatomite composite at different concentrations (CNFs: 1%, 2% and 5%; diatomite: 10% and 30%).
34364565	4	35	theme	films	598:602	arg1	characteristics					556:570	mechanical characteristics	545:570	mechanical characteristics	545:570	The mechanical characteristics and hydrophobicity of the films were observed to be improved after incorporating the copolymerized CNFs/diatomite composite at different concentrations (CNFs: 1%, 2% and 5%; diatomite: 10% and 30%).
34364565	0	36	theme	copolymerized	29:41	arg1	nanofibers					53:62	copolymerized cellulose nanofibers	29:62	copolymerized cellulose nanofibers	29:62	Understanding the effects of copolymerized cellulose nanofibers and diatomite nanocomposite on blend chitosan films.
34364565	6	37	theme	%	1049:1049	arg1	diatomite					1051:1059	30% diatomite	1047:1059	30% diatomite	1047:1059	Moreover, the water solubility decreased from 30% for chitosan control film (CH-0) to 21.06% for films containing 30% diatomite and 5% CNFs (CNFs-D30-5).
34364565	6	37	theme	%	1049:1049	arg1	CNFs-D30-5					1074:1083	CNFs-D30-5	1074:1083	CNFs-D30-5	1074:1083	Moreover, the water solubility decreased from 30% for chitosan control film (CH-0) to 21.06% for films containing 30% diatomite and 5% CNFs (CNFs-D30-5).
34364565	7	38	theme	scanning	1091:1098	arg1	micrographs					1109:1119	The scanning electron micrographs	1087:1119	The scanning electron micrographs	1087:1119	The scanning electron micrographs showed an overall uniform distribution of copolymerized CNFs/diatomite composite in the chitosan matrix with punctual agglomerations.
34364565	2	39	dep	copolymerized	321:333	arg1	monomers					359:366	polyacrylonitrile monomers	341:366	polyacrylonitrile monomers	341:366	Herein, we have produced chitosan composite films supplemented with copolymerized (with polyacrylonitrile monomers) cellulose nanofibers and diatomite nanocomposite at different concentrations.
34364565	0	40	theme	nanofibers	53:62	arg1	effects					18:24	the effects	14:24	the effects of copolymerized cellulose nanofibers and diatomite nanocomposite on blend chitosan films	14:114	Understanding the effects of copolymerized cellulose nanofibers and diatomite nanocomposite on blend chitosan films.
34364565	6	41	theme	water	947:951	arg1	solubility					953:962	the water solubility	943:962	the water solubility	943:962	Moreover, the water solubility decreased from 30% for chitosan control film (CH-0) to 21.06% for films containing 30% diatomite and 5% CNFs (CNFs-D30-5).
34364565	7	42	theme	copolymerized	1163:1175	arg1	composite					1192:1200	copolymerized CNFs/diatomite composite	1163:1200	copolymerized CNFs/diatomite composite	1163:1200	The scanning electron micrographs showed an overall uniform distribution of copolymerized CNFs/diatomite composite in the chitosan matrix with punctual agglomerations.
34364565	4	43	dep	concentrations	709:722	arg1	%					743:743	2% and 5%	735:743	%	743:743	The mechanical characteristics and hydrophobicity of the films were observed to be improved after incorporating the copolymerized CNFs/diatomite composite at different concentrations (CNFs: 1%, 2% and 5%; diatomite: 10% and 30%).
34364565	4	43	dep	concentrations	709:722	arg1	%					732:732	CNFs: 1%	725:732	CNFs: 1%	725:732	The mechanical characteristics and hydrophobicity of the films were observed to be improved after incorporating the copolymerized CNFs/diatomite composite at different concentrations (CNFs: 1%, 2% and 5%; diatomite: 10% and 30%).
34364565	4	43	dep	concentrations	709:722	arg1	%					736:736	2% and 5%	735:743	%	736:736	The mechanical characteristics and hydrophobicity of the films were observed to be improved after incorporating the copolymerized CNFs/diatomite composite at different concentrations (CNFs: 1%, 2% and 5%; diatomite: 10% and 30%).
34364565	7	44	theme	composite	1192:1200	arg1	distribution					1147:1158	an overall uniform distribution	1128:1158	an overall uniform distribution of copolymerized CNFs/diatomite composite in the chitosan matrix	1128:1223	The scanning electron micrographs showed an overall uniform distribution of copolymerized CNFs/diatomite composite in the chitosan matrix with punctual agglomerations.
34364565	4	45	dep	characteristics	556:570	arg1	The					541:543	The	541:543	The	541:543	The mechanical characteristics and hydrophobicity of the films were observed to be improved after incorporating the copolymerized CNFs/diatomite composite at different concentrations (CNFs: 1%, 2% and 5%; diatomite: 10% and 30%).
34364565	7	46	theme	electron	1100:1107	arg1	micrographs					1109:1119	The scanning electron micrographs	1087:1119	The scanning electron micrographs	1087:1119	The scanning electron micrographs showed an overall uniform distribution of copolymerized CNFs/diatomite composite in the chitosan matrix with punctual agglomerations.
34364565	1	47	theme	various	137:143	arg1	properties					171:180	various important physicochemical properties	137:180	various important physicochemical properties	137:180	Chitosan films lack various important physicochemical properties and need to be supplemented with reinforcing agents to bridge the gap.
34364565	6	48	theme	control	996:1002	arg1	film					1004:1007	chitosan control film	987:1007	chitosan control film (CH-0)	987:1014	Moreover, the water solubility decreased from 30% for chitosan control film (CH-0) to 21.06% for films containing 30% diatomite and 5% CNFs (CNFs-D30-5).
34364565	6	48	theme	control	996:1002	arg1	CH-0					1010:1013	CH-0	1010:1013	CH-0	1010:1013	Moreover, the water solubility decreased from 30% for chitosan control film (CH-0) to 21.06% for films containing 30% diatomite and 5% CNFs (CNFs-D30-5).
34364565	6	49	theme	30	1047:1048	arg1	%					1049:1049	%	1049:1049	%	1049:1049	Moreover, the water solubility decreased from 30% for chitosan control film (CH-0) to 21.06% for films containing 30% diatomite and 5% CNFs (CNFs-D30-5).
34364565	1	50	theme	important	145:153	arg1	properties					171:180	various important physicochemical properties	137:180	various important physicochemical properties	137:180	Chitosan films lack various important physicochemical properties and need to be supplemented with reinforcing agents to bridge the gap.
34364565	3	51	theme	films	534:538	arg1	properties					516:525	the physicochemical properties	496:525	the physicochemical properties of the films	496:538	The incorporation of CNFs and diatomite enhanced the physicochemical properties of the films.
34364565	0	52	theme	nanocomposite	78:90	arg1	effects					18:24	the effects	14:24	the effects of copolymerized cellulose nanofibers and diatomite nanocomposite on blend chitosan films	14:114	Understanding the effects of copolymerized cellulose nanofibers and diatomite nanocomposite on blend chitosan films.
34364565	4	53	theme	CNFs	725:728	arg1	%					743:743	2% and 5%	735:743	%	743:743	The mechanical characteristics and hydrophobicity of the films were observed to be improved after incorporating the copolymerized CNFs/diatomite composite at different concentrations (CNFs: 1%, 2% and 5%; diatomite: 10% and 30%).
34364565	4	53	theme	CNFs	725:728	arg1	%					732:732	CNFs: 1%	725:732	CNFs: 1%	725:732	The mechanical characteristics and hydrophobicity of the films were observed to be improved after incorporating the copolymerized CNFs/diatomite composite at different concentrations (CNFs: 1%, 2% and 5%; diatomite: 10% and 30%).
34364565	4	53	theme	CNFs	725:728	arg1	%					736:736	2% and 5%	735:743	%	736:736	The mechanical characteristics and hydrophobicity of the films were observed to be improved after incorporating the copolymerized CNFs/diatomite composite at different concentrations (CNFs: 1%, 2% and 5%; diatomite: 10% and 30%).
34364565	1	54	theme	physicochemical	155:169	arg1	properties					171:180	various important physicochemical properties	137:180	various important physicochemical properties	137:180	Chitosan films lack various important physicochemical properties and need to be supplemented with reinforcing agents to bridge the gap.
34364565	0	55	theme	diatomite	68:76	arg1	nanocomposite					78:90	diatomite nanocomposite	68:90	diatomite nanocomposite	68:90	Understanding the effects of copolymerized cellulose nanofibers and diatomite nanocomposite on blend chitosan films.
34364565	4	56	dep	%	732:732	arg1	%					767:767	30%	765:767	30%	765:767	The mechanical characteristics and hydrophobicity of the films were observed to be improved after incorporating the copolymerized CNFs/diatomite composite at different concentrations (CNFs: 1%, 2% and 5%; diatomite: 10% and 30%).
34364565	4	56	dep	%	732:732	arg1	diatomite					746:754	diatomite	746:754	diatomite	746:754	The mechanical characteristics and hydrophobicity of the films were observed to be improved after incorporating the copolymerized CNFs/diatomite composite at different concentrations (CNFs: 1%, 2% and 5%; diatomite: 10% and 30%).
34364565	4	56	dep	%	732:732	arg1	%					759:759	10%	757:759	10%	757:759	The mechanical characteristics and hydrophobicity of the films were observed to be improved after incorporating the copolymerized CNFs/diatomite composite at different concentrations (CNFs: 1%, 2% and 5%; diatomite: 10% and 30%).
34364565	2	57	theme	diatomite	394:402	arg1	nanocomposite					404:416	diatomite nanocomposite	394:416	diatomite nanocomposite	394:416	Herein, we have produced chitosan composite films supplemented with copolymerized (with polyacrylonitrile monomers) cellulose nanofibers and diatomite nanocomposite at different concentrations.
34364565	4	58	theme	different	699:707	arg1	concentrations					709:722	different concentrations	699:722	different concentrations (CNFs: 1%, 2% and 5%; diatomite: 10% and 30%)	699:768	The mechanical characteristics and hydrophobicity of the films were observed to be improved after incorporating the copolymerized CNFs/diatomite composite at different concentrations (CNFs: 1%, 2% and 5%; diatomite: 10% and 30%).
32564858	8	0	theme	carboxymethyl	986:998	arg1	CML					1009:1011	CML	1009:1011	CML	1009:1011	Furthermore, COS prevented the decrease of serum Nε-(carboxymethyl) lysine (CML) level caused by CML ingestion in a mouse model of diet-induced obesity.
32564858	8	0	theme	carboxymethyl	986:998	arg1	lysine					1001:1006	serum Nε-(carboxymethyl) lysine	976:1006	serum Nε-(carboxymethyl) lysine (CML) level caused by CML ingestion	976:1042	Furthermore, COS prevented the decrease of serum Nε-(carboxymethyl) lysine (CML) level caused by CML ingestion in a mouse model of diet-induced obesity.
32564858	1	1	theme	human	149:153	arg1	healthcare					155:164	human healthcare	149:164	human healthcare	149:164	Chitooligosaccharides (COS) have garnered great attention in the field of human healthcare.
32564858	8	2	theme	obesity	1077:1083	arg1	model					1055:1059	a mouse model	1047:1059	a mouse model of diet-induced obesity	1047:1083	Furthermore, COS prevented the decrease of serum Nε-(carboxymethyl) lysine (CML) level caused by CML ingestion in a mouse model of diet-induced obesity.
32564858	1	3	theme	healthcare	155:164	arg1	field					140:144	the field	136:144	the field of human healthcare	136:164	Chitooligosaccharides (COS) have garnered great attention in the field of human healthcare.
32564858	7	4	theme	acid	908:911	arg1	dose-dependent					917:930	dose-dependent	917:930	dose-dependent	917:930	The antiglycation activity of COS and acetic acid was dose-dependent.
32564858	7	4	theme	acid	908:911	arg1	activity					881:888	The antiglycation activity	863:888	The antiglycation activity of COS and acetic acid	863:911	The antiglycation activity of COS and acetic acid was dose-dependent.
32564858	0	5	from	Effect	0:5	arg1	antiglycation					60:72	antiglycation	60:72	antiglycation	60:72	Effect of chitooligosaccharides on human gut microbiota and antiglycation.
32564858	0	5	from	Effect	0:5	arg1	microbiota					45:54	human gut microbiota	35:54	human gut microbiota	35:54	Effect of chitooligosaccharides on human gut microbiota and antiglycation.
32564858	8	6	theme	mouse	1049:1053	arg1	model					1055:1059	a mouse model	1047:1059	a mouse model of diet-induced obesity	1047:1083	Furthermore, COS prevented the decrease of serum Nε-(carboxymethyl) lysine (CML) level caused by CML ingestion in a mouse model of diet-induced obesity.
32564858	5	7	theme	gut	578:580	arg1	composition					593:603	the gut microbiota composition	574:603	the gut microbiota composition	574:603	In addition, COS modulated the gut microbiota composition by increasing Bacteroidetes, decreasing Proteobacteria and Actinobacteria, and lowering the Firmicutes/Bacteroidetes ratio.
32564858	2	8	theme	studies	279:285	arg1	combination					243:253	a combination	241:253	a combination of in vitro and in vivo studies	241:285	The prebiotic activities and antiglycation of COS were investigated using a combination of in vitro and in vivo studies.
32564858	5	9	theme	microbiota	582:591	arg1	composition					593:603	the gut microbiota composition	574:603	the gut microbiota composition	574:603	In addition, COS modulated the gut microbiota composition by increasing Bacteroidetes, decreasing Proteobacteria and Actinobacteria, and lowering the Firmicutes/Bacteroidetes ratio.
32564858	9	10	theme	host	1097:1100	arg1	health					1102:1107	host health	1097:1107	host health	1097:1107	To improve host health, COS could be potential prebiotics in food products.
32564858	3	11	theme	acetic	345:350	arg1	acid					352:355	acetic acid	345:355	acetic acid	345:355	COS supplementation dramatically increased the levels of acetic acid, while reducing the concentrations of propionic and butyric acids.
32564858	6	12	theme	beneficial	760:769	arg1	Bacteroides					771:781	beneficial Bacteroides	760:781	beneficial Bacteroides	760:781	COS promoted the generation of beneficial Bacteroides and Faecalibacterium genera, while suppressing the pathogenic Klebsiella genus.
32564858	2	13	theme	in	271:272	arg1	studies					279:285	in vitro and in vivo studies	258:285	in vitro and in vivo studies	258:285	The prebiotic activities and antiglycation of COS were investigated using a combination of in vitro and in vivo studies.
32564858	7	14	theme	acetic	901:906	arg1	acid					908:911	acetic acid	901:911	acetic acid	901:911	The antiglycation activity of COS and acetic acid was dose-dependent.
32564858	3	15	theme	acid	352:355	arg1	levels					335:340	the levels	331:340	the levels of acetic acid	331:355	COS supplementation dramatically increased the levels of acetic acid, while reducing the concentrations of propionic and butyric acids.
32564858	0	16	theme	gut	41:43	arg1	microbiota					45:54	human gut microbiota	35:54	human gut microbiota	35:54	Effect of chitooligosaccharides on human gut microbiota and antiglycation.
32564858	8	17	theme	CML	1030:1032	arg1	ingestion					1034:1042	CML ingestion	1030:1042	CML ingestion	1030:1042	Furthermore, COS prevented the decrease of serum Nε-(carboxymethyl) lysine (CML) level caused by CML ingestion in a mouse model of diet-induced obesity.
32564858	8	18	theme	level	1014:1018	arg1	decrease					964:971	the decrease	960:971	the decrease of serum Nε-(carboxymethyl) lysine (CML) level caused by CML ingestion	960:1042	Furthermore, COS prevented the decrease of serum Nε-(carboxymethyl) lysine (CML) level caused by CML ingestion in a mouse model of diet-induced obesity.
32564858	7	19	theme	COS	893:895	arg1	dose-dependent					917:930	dose-dependent	917:930	dose-dependent	917:930	The antiglycation activity of COS and acetic acid was dose-dependent.
32564858	7	19	theme	COS	893:895	arg1	activity					881:888	The antiglycation activity	863:888	The antiglycation activity of COS and acetic acid	863:911	The antiglycation activity of COS and acetic acid was dose-dependent.
32564858	4	20	theme	microbiota	535:544	arg1	richness					515:522	richness	515:522	richness	515:522	It also decreased the total bacterial population; however, it did not affect diversity and richness of the gut microbiota.
32564858	4	20	theme	microbiota	535:544	arg1	diversity					501:509	diversity	501:509	diversity	501:509	It also decreased the total bacterial population; however, it did not affect diversity and richness of the gut microbiota.
32564858	8	21	theme	diet-induced	1064:1075	arg1	obesity					1077:1083	diet-induced obesity	1064:1083	diet-induced obesity	1064:1083	Furthermore, COS prevented the decrease of serum Nε-(carboxymethyl) lysine (CML) level caused by CML ingestion in a mouse model of diet-induced obesity.
32564858	8	22	theme	lysine	1001:1006	arg1	level					1014:1018	serum Nε-(carboxymethyl) lysine (CML) level	976:1018	serum Nε-(carboxymethyl) lysine (CML) level caused by CML ingestion	976:1042	Furthermore, COS prevented the decrease of serum Nε-(carboxymethyl) lysine (CML) level caused by CML ingestion in a mouse model of diet-induced obesity.
32564858	7	23	theme	antiglycation	867:879	arg1	dose-dependent					917:930	dose-dependent	917:930	dose-dependent	917:930	The antiglycation activity of COS and acetic acid was dose-dependent.
32564858	7	23	theme	antiglycation	867:879	arg1	activity					881:888	The antiglycation activity	863:888	The antiglycation activity of COS and acetic acid	863:911	The antiglycation activity of COS and acetic acid was dose-dependent.
32564858	2	24	dep	in	258:259	arg1	vitro					261:265	vitro	261:265	vitro	261:265	The prebiotic activities and antiglycation of COS were investigated using a combination of in vitro and in vivo studies.
32564858	4	25	theme	gut	531:533	arg1	microbiota					535:544	the gut microbiota	527:544	the gut microbiota	527:544	It also decreased the total bacterial population; however, it did not affect diversity and richness of the gut microbiota.
32564858	5	26	theme	Firmicutes/Bacteroidetes	697:720	arg1	ratio					722:726	the Firmicutes/Bacteroidetes ratio	693:726	the Firmicutes/Bacteroidetes ratio	693:726	In addition, COS modulated the gut microbiota composition by increasing Bacteroidetes, decreasing Proteobacteria and Actinobacteria, and lowering the Firmicutes/Bacteroidetes ratio.
32564858	6	27	theme	genera	804:809	arg1	generation					746:755	the generation	742:755	the generation of beneficial Bacteroides and Faecalibacterium genera	742:809	COS promoted the generation of beneficial Bacteroides and Faecalibacterium genera, while suppressing the pathogenic Klebsiella genus.
32564858	9	28	from	prebiotics	1133:1142	arg1	products					1152:1159	food products	1147:1159	food products	1147:1159	To improve host health, COS could be potential prebiotics in food products.
32564858	0	29	theme	chitooligosaccharides	10:30	arg1	Effect					0:5	Effect	0:5	Effect of chitooligosaccharides on human gut microbiota and antiglycation.	0:73	Effect of chitooligosaccharides on human gut microbiota and antiglycation.
32564858	8	30	theme	serum	976:980	arg1	CML					1009:1011	CML	1009:1011	CML	1009:1011	Furthermore, COS prevented the decrease of serum Nε-(carboxymethyl) lysine (CML) level caused by CML ingestion in a mouse model of diet-induced obesity.
32564858	8	30	theme	serum	976:980	arg1	lysine					1001:1006	serum Nε-(carboxymethyl) lysine	976:1006	serum Nε-(carboxymethyl) lysine (CML) level caused by CML ingestion	976:1042	Furthermore, COS prevented the decrease of serum Nε-(carboxymethyl) lysine (CML) level caused by CML ingestion in a mouse model of diet-induced obesity.
32564858	4	31	theme	total	446:450	arg1	population					462:471	the total bacterial population	442:471	the total bacterial population	442:471	It also decreased the total bacterial population; however, it did not affect diversity and richness of the gut microbiota.
32564858	3	32	theme	butyric	409:415	arg1	acids					417:421	propionic and butyric acids	395:421	propionic and butyric acids	395:421	COS supplementation dramatically increased the levels of acetic acid, while reducing the concentrations of propionic and butyric acids.
32564858	2	33	theme	prebiotic	171:179	arg1	activities					181:190	The prebiotic activities	167:190	The prebiotic activities	167:190	The prebiotic activities and antiglycation of COS were investigated using a combination of in vitro and in vivo studies.
32564858	0	34	theme	human	35:39	arg1	microbiota					45:54	human gut microbiota	35:54	human gut microbiota	35:54	Effect of chitooligosaccharides on human gut microbiota and antiglycation.
32564858	3	35	theme	acids	417:421	arg1	concentrations					377:390	the concentrations	373:390	the concentrations of propionic and butyric acids	373:421	COS supplementation dramatically increased the levels of acetic acid, while reducing the concentrations of propionic and butyric acids.
32564858	6	36	theme	Faecalibacterium	787:802	arg1	genera					804:809	Faecalibacterium genera	787:809	Faecalibacterium genera	787:809	COS promoted the generation of beneficial Bacteroides and Faecalibacterium genera, while suppressing the pathogenic Klebsiella genus.
32564858	3	37	theme	propionic	395:403	arg1	acids					417:421	propionic and butyric acids	395:421	propionic and butyric acids	395:421	COS supplementation dramatically increased the levels of acetic acid, while reducing the concentrations of propionic and butyric acids.
32564858	6	38	theme	pathogenic	834:843	arg1	genus					856:860	the pathogenic Klebsiella genus	830:860	the pathogenic Klebsiella genus	830:860	COS promoted the generation of beneficial Bacteroides and Faecalibacterium genera, while suppressing the pathogenic Klebsiella genus.
32564858	4	39	theme	bacterial	452:460	arg1	population					462:471	the total bacterial population	442:471	the total bacterial population	442:471	It also decreased the total bacterial population; however, it did not affect diversity and richness of the gut microbiota.
32564858	9	40	theme	potential	1123:1131	arg1	prebiotics					1133:1142	potential prebiotics	1123:1142	potential prebiotics in food products	1123:1159	To improve host health, COS could be potential prebiotics in food products.
32564858	1	41	theme	great	117:121	arg1	attention					123:131	great attention	117:131	great attention	117:131	Chitooligosaccharides (COS) have garnered great attention in the field of human healthcare.
32564858	2	42	theme	COS	213:215	arg1	antiglycation					196:208	antiglycation	196:208	antiglycation of COS	196:215	The prebiotic activities and antiglycation of COS were investigated using a combination of in vitro and in vivo studies.
32564858	2	42	theme	COS	213:215	arg1	activities					181:190	The prebiotic activities	167:190	The prebiotic activities	167:190	The prebiotic activities and antiglycation of COS were investigated using a combination of in vitro and in vivo studies.
32564858	6	43	theme	Bacteroides	771:781	arg1	generation					746:755	the generation	742:755	the generation of beneficial Bacteroides and Faecalibacterium genera	742:809	COS promoted the generation of beneficial Bacteroides and Faecalibacterium genera, while suppressing the pathogenic Klebsiella genus.
32564858	6	44	theme	Klebsiella	845:854	arg1	genus					856:860	the pathogenic Klebsiella genus	830:860	the pathogenic Klebsiella genus	830:860	COS promoted the generation of beneficial Bacteroides and Faecalibacterium genera, while suppressing the pathogenic Klebsiella genus.
32564858	3	45	theme	COS	288:290	arg1	supplementation					292:306	COS supplementation	288:306	COS supplementation	288:306	COS supplementation dramatically increased the levels of acetic acid, while reducing the concentrations of propionic and butyric acids.
32564858	2	46	theme	in	258:259	arg1	studies					279:285	in vitro and in vivo studies	258:285	in vitro and in vivo studies	258:285	The prebiotic activities and antiglycation of COS were investigated using a combination of in vitro and in vivo studies.
32564858	9	47	theme	food	1147:1150	arg1	products					1152:1159	food products	1147:1159	food products	1147:1159	To improve host health, COS could be potential prebiotics in food products.
32564858	8	48	theme	Nε-	982:984	arg1	CML					1009:1011	CML	1009:1011	CML	1009:1011	Furthermore, COS prevented the decrease of serum Nε-(carboxymethyl) lysine (CML) level caused by CML ingestion in a mouse model of diet-induced obesity.
32564858	8	48	theme	Nε-	982:984	arg1	lysine					1001:1006	serum Nε-(carboxymethyl) lysine	976:1006	serum Nε-(carboxymethyl) lysine (CML) level caused by CML ingestion	976:1042	Furthermore, COS prevented the decrease of serum Nε-(carboxymethyl) lysine (CML) level caused by CML ingestion in a mouse model of diet-induced obesity.
32564858	2	49	dep	in	271:272	arg1	vivo					274:277	vivo	274:277	vivo	274:277	The prebiotic activities and antiglycation of COS were investigated using a combination of in vitro and in vivo studies.
34586150	4	0	theme	ionic	942:946	arg1	conductivity					948:959	high ionic conductivity	937:959	high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz)	937:991	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	3	1	theme	DN	513:514	arg1	hydrogel					516:523	a novel DN hydrogel	505:523	a novel DN hydrogel composed fully of natural polymers exhibiting remarkable mechanical properties and conductivity	505:619	Here, a novel DN hydrogel composed fully of natural polymers exhibiting remarkable mechanical properties and conductivity is prepared by simply soaking a virgin gellan gum/gelatin composite hydrogel in a mixed solution of Na2SO4 and (NH4)2SO4.
34586150	3	2	theme	novel	507:511	arg1	hydrogel					516:523	a novel DN hydrogel	505:523	a novel DN hydrogel composed fully of natural polymers exhibiting remarkable mechanical properties and conductivity	505:619	Here, a novel DN hydrogel composed fully of natural polymers exhibiting remarkable mechanical properties and conductivity is prepared by simply soaking a virgin gellan gum/gelatin composite hydrogel in a mixed solution of Na2SO4 and (NH4)2SO4.
34586150	4	3	theme	fracture	817:824	arg1	stress					826:831	good fracture stress	812:831	good fracture stress (0.05 to 7.5 MPa)	812:849	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	4	3	theme	fracture	817:824	arg1	MPa					846:848	0.05 to 7.5 MPa	834:848	0.05 to 7.5 MPa	834:848	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	6	4	theme	concentration	1372:1384	arg1	solution					1355:1362	a salt solution	1348:1362	a salt solution of high concentration	1348:1384	The high ionic conductivity of the DN gel is attributed to the infiltration of the DN gel in a salt solution of high concentration.
34586150	1	5	theme	soft	167:170	arg1	robotics					172:179	soft robotics	167:179	soft robotics	167:179	Hydrogels are widely used in fields such as drug delivery, tissue regeneration, soft robotics and flexible smart electronic devices, yet their application is often limited by unsatisfactory mechanical behaviors.
34586150	2	6	theme	various	309:315	arg1	strategies					329:338	the various improvement strategies	305:338	the various improvement strategies	305:338	Among the various improvement strategies, double network (DN) hydrogels from synthetic polymers demonstrated impressive mechanical properties, while those from natural polymers were usually inferior.
34586150	4	7	from	kHz	988:990	arg1	m-1					975:977	up to 11.4 S m-1	962:977	up to 11.4 S m-1 at f = 1 kHz	962:990	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	3	8	theme	remarkable	571:580	arg1	properties					593:602	remarkable mechanical properties	571:602	remarkable mechanical properties	571:602	Here, a novel DN hydrogel composed fully of natural polymers exhibiting remarkable mechanical properties and conductivity is prepared by simply soaking a virgin gellan gum/gelatin composite hydrogel in a mixed solution of Na2SO4 and (NH4)2SO4.
34586150	6	9	theme	salt	1350:1353	arg1	solution					1355:1362	a salt solution	1348:1362	a salt solution of high concentration	1348:1384	The high ionic conductivity of the DN gel is attributed to the infiltration of the DN gel in a salt solution of high concentration.
34586150	1	10	theme	smart	194:198	arg1	devices					211:217	flexible smart electronic devices	185:217	flexible smart electronic devices	185:217	Hydrogels are widely used in fields such as drug delivery, tissue regeneration, soft robotics and flexible smart electronic devices, yet their application is often limited by unsatisfactory mechanical behaviors.
34586150	7	11	theme	DN	1500:1501	arg1	hydrogels					1503:1511	natural-polymer-based DN hydrogels	1478:1511	natural-polymer-based DN hydrogels	1478:1511	The developed gellan gum/gelatin DN hydrogel has shown a new pathway towards strengthening natural-polymer-based DN hydrogels and towards potential applications in biomedical engineering and flexible electronic devices.
34586150	4	12	theme	=	984:984	arg1	kHz					988:990	f = 1 kHz	982:990	f = 1 kHz	982:990	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	4	13	dep	7.1	882:884	arg1	to					879:880	to	879:880	to	879:880	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	4	14	dep	42.6	801:804	arg1	to					798:799	to	798:799	to	798:799	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	7	15	from	applications	1535:1546	arg1	engineering					1562:1572	biomedical engineering	1551:1572	biomedical engineering	1551:1572	The developed gellan gum/gelatin DN hydrogel has shown a new pathway towards strengthening natural-polymer-based DN hydrogels and towards potential applications in biomedical engineering and flexible electronic devices.
34586150	7	15	from	applications	1535:1546	arg1	devices					1598:1604	flexible electronic devices	1578:1604	flexible electronic devices	1578:1604	The developed gellan gum/gelatin DN hydrogel has shown a new pathway towards strengthening natural-polymer-based DN hydrogels and towards potential applications in biomedical engineering and flexible electronic devices.
34586150	5	16	from	improvement	998:1008	arg1	properties					1028:1037	the mechanical properties	1013:1037	the mechanical properties of the DN gel	1013:1051	The improvement in the mechanical properties of the DN gel is attributed to the chain-entanglement crosslinking points introduced by SO42- in the gelatin network and the electrostatic interaction crosslinking points introduced by Na+ in the gellan gum network.
34586150	4	17	theme	fracture	857:864	arg1	7.1					882:884	7.1	882:884	7.1	882:884	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	4	17	theme	fracture	857:864	arg1	stretch					866:872	good fracture stretch	852:872	good fracture stretch (1.4 to 7.1)	852:885	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	5	18	theme	gelatin	1140:1146	arg1	network					1148:1154	the gelatin network	1136:1154	the gelatin network	1136:1154	The improvement in the mechanical properties of the DN gel is attributed to the chain-entanglement crosslinking points introduced by SO42- in the gelatin network and the electrostatic interaction crosslinking points introduced by Na+ in the gellan gum network.
34586150	1	19	theme	drug	131:134	arg1	delivery					136:143	drug delivery	131:143	drug delivery	131:143	Hydrogels are widely used in fields such as drug delivery, tissue regeneration, soft robotics and flexible smart electronic devices, yet their application is often limited by unsatisfactory mechanical behaviors.
34586150	0	20	theme	network	69:75	arg1	hydrogel					77:84	An extremely tough and ionic conductive natural-polymer-based double network hydrogel	0:84	An extremely tough and ionic conductive natural-polymer-based double network hydrogel.	0:85	An extremely tough and ionic conductive natural-polymer-based double network hydrogel.
34586150	6	21	theme	DN	1290:1291	arg1	gel					1293:1295	the DN gel	1286:1295	the DN gel	1286:1295	The high ionic conductivity of the DN gel is attributed to the infiltration of the DN gel in a salt solution of high concentration.
34586150	7	22	theme	electronic	1587:1596	arg1	devices					1598:1604	flexible electronic devices	1578:1604	flexible electronic devices	1578:1604	The developed gellan gum/gelatin DN hydrogel has shown a new pathway towards strengthening natural-polymer-based DN hydrogels and towards potential applications in biomedical engineering and flexible electronic devices.
34586150	7	23	theme	DN	1420:1421	arg1	hydrogel					1423:1430	The developed gellan gum/gelatin DN hydrogel	1387:1430	The developed gellan gum/gelatin DN hydrogel	1387:1430	The developed gellan gum/gelatin DN hydrogel has shown a new pathway towards strengthening natural-polymer-based DN hydrogels and towards potential applications in biomedical engineering and flexible electronic devices.
34586150	4	24	theme	tunable	768:774	arg1	MPa					806:808	0.08 to 42.6 MPa	793:808	0.08 to 42.6 MPa	793:808	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	4	24	theme	tunable	768:774	arg1	modulus					784:790	a tunable Young's modulus	766:790	a tunable Young's modulus (0.08 to 42.6 MPa)	766:809	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	4	25	dep	conductivity	948:959	arg1	m-1					975:977	up to 11.4 S m-1	962:977	up to 11.4 S m-1 at f = 1 kHz	962:990	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	7	26	theme	gellan	1401:1406	arg1	hydrogel					1423:1430	The developed gellan gum/gelatin DN hydrogel	1387:1430	The developed gellan gum/gelatin DN hydrogel	1387:1430	The developed gellan gum/gelatin DN hydrogel has shown a new pathway towards strengthening natural-polymer-based DN hydrogels and towards potential applications in biomedical engineering and flexible electronic devices.
34586150	4	27	theme	high	888:891	arg1	toughness					902:910	high fracture toughness	888:910	high fracture toughness (up to 27.7 kJ m-2)	888:930	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	4	27	theme	high	888:891	arg1	m-2					927:929	up to 27.7 kJ m-2	913:929	up to 27.7 kJ m-2	913:929	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	4	28	dep	m-1	975:977	arg1	S					973:973	S	973:973	S	973:973	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	7	29	theme	biomedical	1551:1560	arg1	engineering					1562:1572	biomedical engineering	1551:1572	biomedical engineering	1551:1572	The developed gellan gum/gelatin DN hydrogel has shown a new pathway towards strengthening natural-polymer-based DN hydrogels and towards potential applications in biomedical engineering and flexible electronic devices.
34586150	2	30	theme	natural	459:465	arg1	polymers					467:474	natural polymers	459:474	natural polymers	459:474	Among the various improvement strategies, double network (DN) hydrogels from synthetic polymers demonstrated impressive mechanical properties, while those from natural polymers were usually inferior.
34586150	2	31	theme	synthetic	376:384	arg1	polymers					386:393	synthetic polymers	376:393	synthetic polymers	376:393	Among the various improvement strategies, double network (DN) hydrogels from synthetic polymers demonstrated impressive mechanical properties, while those from natural polymers were usually inferior.
34586150	1	32	theme	unsatisfactory	262:275	arg1	behaviors					288:296	unsatisfactory mechanical behaviors	262:296	unsatisfactory mechanical behaviors	262:296	Hydrogels are widely used in fields such as drug delivery, tissue regeneration, soft robotics and flexible smart electronic devices, yet their application is often limited by unsatisfactory mechanical behaviors.
34586150	4	33	dep	11.4	968:971	arg1	to					965:966	to	965:966	to	965:966	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	4	34	theme	high	937:940	arg1	conductivity					948:959	high ionic conductivity	937:959	high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz)	937:991	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	5	35	theme	electrostatic	1164:1176	arg1	points					1203:1208	the electrostatic interaction crosslinking points	1160:1208	the electrostatic interaction crosslinking points introduced by Na+ in the gellan gum network	1160:1252	The improvement in the mechanical properties of the DN gel is attributed to the chain-entanglement crosslinking points introduced by SO42- in the gelatin network and the electrostatic interaction crosslinking points introduced by Na+ in the gellan gum network.
34586150	0	36	theme	tough	13:17	arg1	hydrogel					77:84	An extremely tough and ionic conductive natural-polymer-based double network hydrogel	0:84	An extremely tough and ionic conductive natural-polymer-based double network hydrogel.	0:85	An extremely tough and ionic conductive natural-polymer-based double network hydrogel.
34586150	3	37	theme	composite	679:687	arg1	hydrogel					689:696	a virgin gellan gum/gelatin composite hydrogel	651:696	a virgin gellan gum/gelatin composite hydrogel	651:696	Here, a novel DN hydrogel composed fully of natural polymers exhibiting remarkable mechanical properties and conductivity is prepared by simply soaking a virgin gellan gum/gelatin composite hydrogel in a mixed solution of Na2SO4 and (NH4)2SO4.
34586150	5	38	theme	crosslinking	1190:1201	arg1	points					1203:1208	the electrostatic interaction crosslinking points	1160:1208	the electrostatic interaction crosslinking points introduced by Na+ in the gellan gum network	1160:1252	The improvement in the mechanical properties of the DN gel is attributed to the chain-entanglement crosslinking points introduced by SO42- in the gelatin network and the electrostatic interaction crosslinking points introduced by Na+ in the gellan gum network.
34586150	2	39	theme	mechanical	419:428	arg1	properties					430:439	impressive mechanical properties	408:439	impressive mechanical properties	408:439	Among the various improvement strategies, double network (DN) hydrogels from synthetic polymers demonstrated impressive mechanical properties, while those from natural polymers were usually inferior.
34586150	0	40	theme	ionic	23:27	arg1	hydrogel					77:84	An extremely tough and ionic conductive natural-polymer-based double network hydrogel	0:84	An extremely tough and ionic conductive natural-polymer-based double network hydrogel.	0:85	An extremely tough and ionic conductive natural-polymer-based double network hydrogel.
34586150	4	41	dep	7.5	842:844	arg1	to					839:840	to	839:840	to	839:840	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	3	42	theme	gellan	660:665	arg1	hydrogel					689:696	a virgin gellan gum/gelatin composite hydrogel	651:696	a virgin gellan gum/gelatin composite hydrogel	651:696	Here, a novel DN hydrogel composed fully of natural polymers exhibiting remarkable mechanical properties and conductivity is prepared by simply soaking a virgin gellan gum/gelatin composite hydrogel in a mixed solution of Na2SO4 and (NH4)2SO4.
34586150	2	43	theme	network	348:354	arg1	hydrogels					361:369	double network (DN) hydrogels	341:369	double network (DN) hydrogels from synthetic polymers	341:393	Among the various improvement strategies, double network (DN) hydrogels from synthetic polymers demonstrated impressive mechanical properties, while those from natural polymers were usually inferior.
34586150	0	44	theme	natural-polymer-based	40:60	arg1	hydrogel					77:84	An extremely tough and ionic conductive natural-polymer-based double network hydrogel	0:84	An extremely tough and ionic conductive natural-polymer-based double network hydrogel.	0:85	An extremely tough and ionic conductive natural-polymer-based double network hydrogel.
34586150	1	45	used	used	108:111	arg2	Hydrogels					87:95	Hydrogels	87:95	Hydrogels	87:95	Hydrogels are widely used in fields such as drug delivery, tissue regeneration, soft robotics and flexible smart electronic devices, yet their application is often limited by unsatisfactory mechanical behaviors.
34586150	3	46	theme	mixed	703:707	arg1	solution					709:716	a mixed solution	701:716	a mixed solution of Na2SO4 and (NH4)2SO4	701:740	Here, a novel DN hydrogel composed fully of natural polymers exhibiting remarkable mechanical properties and conductivity is prepared by simply soaking a virgin gellan gum/gelatin composite hydrogel in a mixed solution of Na2SO4 and (NH4)2SO4.
34586150	6	47	theme	gel	1341:1343	arg1	infiltration					1318:1329	the infiltration	1314:1329	the infiltration of the DN gel in a salt solution of high concentration	1314:1384	The high ionic conductivity of the DN gel is attributed to the infiltration of the DN gel in a salt solution of high concentration.
34586150	5	48	theme	crosslinking	1093:1104	arg1	points					1106:1111	the chain-entanglement crosslinking points	1070:1111	the chain-entanglement crosslinking points introduced by SO42- in the gelatin network and the electrostatic interaction crosslinking points introduced by Na+ in the gellan gum network	1070:1252	The improvement in the mechanical properties of the DN gel is attributed to the chain-entanglement crosslinking points introduced by SO42- in the gelatin network and the electrostatic interaction crosslinking points introduced by Na+ in the gellan gum network.
34586150	6	49	from	infiltration	1318:1329	arg1	solution					1355:1362	a salt solution	1348:1362	a salt solution of high concentration	1348:1384	The high ionic conductivity of the DN gel is attributed to the infiltration of the DN gel in a salt solution of high concentration.
34586150	5	50	theme	gum	1242:1244	arg1	network					1246:1252	the gellan gum network	1231:1252	the gellan gum network	1231:1252	The improvement in the mechanical properties of the DN gel is attributed to the chain-entanglement crosslinking points introduced by SO42- in the gelatin network and the electrostatic interaction crosslinking points introduced by Na+ in the gellan gum network.
34586150	2	51	theme	DN	357:358	arg1	hydrogels					361:369	double network (DN) hydrogels	341:369	double network (DN) hydrogels from synthetic polymers	341:393	Among the various improvement strategies, double network (DN) hydrogels from synthetic polymers demonstrated impressive mechanical properties, while those from natural polymers were usually inferior.
34586150	5	52	theme	gel	1049:1051	arg1	properties					1028:1037	the mechanical properties	1013:1037	the mechanical properties of the DN gel	1013:1051	The improvement in the mechanical properties of the DN gel is attributed to the chain-entanglement crosslinking points introduced by SO42- in the gelatin network and the electrostatic interaction crosslinking points introduced by Na+ in the gellan gum network.
34586150	4	53	dep	27.7	919:922	arg1	to					916:917	to	916:917	to	916:917	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	3	54	theme	natural	543:549	arg1	polymers					551:558	natural polymers	543:558	natural polymers exhibiting remarkable mechanical properties and conductivity	543:619	Here, a novel DN hydrogel composed fully of natural polymers exhibiting remarkable mechanical properties and conductivity is prepared by simply soaking a virgin gellan gum/gelatin composite hydrogel in a mixed solution of Na2SO4 and (NH4)2SO4.
34586150	2	55	theme	improvement	317:327	arg1	strategies					329:338	the various improvement strategies	305:338	the various improvement strategies	305:338	Among the various improvement strategies, double network (DN) hydrogels from synthetic polymers demonstrated impressive mechanical properties, while those from natural polymers were usually inferior.
34586150	6	56	theme	ionic	1264:1268	arg1	conductivity					1270:1281	The high ionic conductivity	1255:1281	The high ionic conductivity of the DN gel	1255:1295	The high ionic conductivity of the DN gel is attributed to the infiltration of the DN gel in a salt solution of high concentration.
34586150	4	57	theme	good	812:815	arg1	stress					826:831	good fracture stress	812:831	good fracture stress (0.05 to 7.5 MPa)	812:849	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	4	57	theme	good	812:815	arg1	MPa					846:848	0.05 to 7.5 MPa	834:848	0.05 to 7.5 MPa	834:848	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	3	58	theme	NH4	733:735	arg1	solution					709:716	a mixed solution	701:716	a mixed solution of Na2SO4 and (NH4)2SO4	701:740	Here, a novel DN hydrogel composed fully of natural polymers exhibiting remarkable mechanical properties and conductivity is prepared by simply soaking a virgin gellan gum/gelatin composite hydrogel in a mixed solution of Na2SO4 and (NH4)2SO4.
34586150	3	59	theme	mechanical	582:591	arg1	properties					593:602	remarkable mechanical properties	571:602	remarkable mechanical properties	571:602	Here, a novel DN hydrogel composed fully of natural polymers exhibiting remarkable mechanical properties and conductivity is prepared by simply soaking a virgin gellan gum/gelatin composite hydrogel in a mixed solution of Na2SO4 and (NH4)2SO4.
34586150	6	60	theme	high	1367:1370	arg1	concentration					1372:1384	high concentration	1367:1384	high concentration	1367:1384	The high ionic conductivity of the DN gel is attributed to the infiltration of the DN gel in a salt solution of high concentration.
34586150	4	61	theme	f	982:982	arg1	kHz					988:990	f = 1 kHz	982:990	f = 1 kHz	982:990	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	1	62	theme	flexible	185:192	arg1	devices					211:217	flexible smart electronic devices	185:217	flexible smart electronic devices	185:217	Hydrogels are widely used in fields such as drug delivery, tissue regeneration, soft robotics and flexible smart electronic devices, yet their application is often limited by unsatisfactory mechanical behaviors.
34586150	7	63	theme	natural-polymer-based	1478:1498	arg1	hydrogels					1503:1511	natural-polymer-based DN hydrogels	1478:1511	natural-polymer-based DN hydrogels	1478:1511	The developed gellan gum/gelatin DN hydrogel has shown a new pathway towards strengthening natural-polymer-based DN hydrogels and towards potential applications in biomedical engineering and flexible electronic devices.
34586150	1	64	theme	electronic	200:209	arg1	devices					211:217	flexible smart electronic devices	185:217	flexible smart electronic devices	185:217	Hydrogels are widely used in fields such as drug delivery, tissue regeneration, soft robotics and flexible smart electronic devices, yet their application is often limited by unsatisfactory mechanical behaviors.
34586150	7	65	theme	new	1444:1446	arg1	pathway					1448:1454	a new pathway	1442:1454	a new pathway towards strengthening natural-polymer-based DN hydrogels and towards potential applications in biomedical engineering and flexible electronic devices	1442:1604	The developed gellan gum/gelatin DN hydrogel has shown a new pathway towards strengthening natural-polymer-based DN hydrogels and towards potential applications in biomedical engineering and flexible electronic devices.
34586150	4	66	theme	good	852:855	arg1	7.1					882:884	7.1	882:884	7.1	882:884	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	4	66	theme	good	852:855	arg1	stretch					866:872	good fracture stretch	852:872	good fracture stretch (1.4 to 7.1)	852:885	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	5	67	from	Na+	1224:1226	arg1	network					1246:1252	the gellan gum network	1231:1252	the gellan gum network	1231:1252	The improvement in the mechanical properties of the DN gel is attributed to the chain-entanglement crosslinking points introduced by SO42- in the gelatin network and the electrostatic interaction crosslinking points introduced by Na+ in the gellan gum network.
34586150	6	68	theme	high	1259:1262	arg1	conductivity					1270:1281	The high ionic conductivity	1255:1281	The high ionic conductivity of the DN gel	1255:1295	The high ionic conductivity of the DN gel is attributed to the infiltration of the DN gel in a salt solution of high concentration.
34586150	4	69	theme	fracture	893:900	arg1	toughness					902:910	high fracture toughness	888:910	high fracture toughness (up to 27.7 kJ m-2)	888:930	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	4	69	theme	fracture	893:900	arg1	m-2					927:929	up to 27.7 kJ m-2	913:929	up to 27.7 kJ m-2	913:929	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	1	70	theme	tissue	146:151	arg1	regeneration					153:164	tissue regeneration	146:164	tissue regeneration	146:164	Hydrogels are widely used in fields such as drug delivery, tissue regeneration, soft robotics and flexible smart electronic devices, yet their application is often limited by unsatisfactory mechanical behaviors.
34586150	7	71	theme	gum/gelatin	1408:1418	arg1	hydrogel					1423:1430	The developed gellan gum/gelatin DN hydrogel	1387:1430	The developed gellan gum/gelatin DN hydrogel	1387:1430	The developed gellan gum/gelatin DN hydrogel has shown a new pathway towards strengthening natural-polymer-based DN hydrogels and towards potential applications in biomedical engineering and flexible electronic devices.
34586150	7	72	theme	developed	1391:1399	arg1	hydrogel					1423:1430	The developed gellan gum/gelatin DN hydrogel	1387:1430	The developed gellan gum/gelatin DN hydrogel	1387:1430	The developed gellan gum/gelatin DN hydrogel has shown a new pathway towards strengthening natural-polymer-based DN hydrogels and towards potential applications in biomedical engineering and flexible electronic devices.
34586150	7	73	theme	flexible	1578:1585	arg1	devices					1598:1604	flexible electronic devices	1578:1604	flexible electronic devices	1578:1604	The developed gellan gum/gelatin DN hydrogel has shown a new pathway towards strengthening natural-polymer-based DN hydrogels and towards potential applications in biomedical engineering and flexible electronic devices.
34586150	4	74	theme	Young	776:780	arg1	MPa					806:808	0.08 to 42.6 MPa	793:808	0.08 to 42.6 MPa	793:808	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	4	74	theme	Young	776:780	arg1	modulus					784:790	a tunable Young's modulus	766:790	a tunable Young's modulus (0.08 to 42.6 MPa)	766:809	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	7	75	from	hydrogels	1503:1511	arg1	engineering					1562:1572	biomedical engineering	1551:1572	biomedical engineering	1551:1572	The developed gellan gum/gelatin DN hydrogel has shown a new pathway towards strengthening natural-polymer-based DN hydrogels and towards potential applications in biomedical engineering and flexible electronic devices.
34586150	7	75	from	hydrogels	1503:1511	arg1	devices					1598:1604	flexible electronic devices	1578:1604	flexible electronic devices	1578:1604	The developed gellan gum/gelatin DN hydrogel has shown a new pathway towards strengthening natural-polymer-based DN hydrogels and towards potential applications in biomedical engineering and flexible electronic devices.
34586150	4	76	dep	S	973:973	arg1	11.4					968:971	11.4	968:971	11.4	968:971	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	6	77	theme	gel	1293:1295	arg1	conductivity					1270:1281	The high ionic conductivity	1255:1281	The high ionic conductivity of the DN gel	1255:1295	The high ionic conductivity of the DN gel is attributed to the infiltration of the DN gel in a salt solution of high concentration.
34586150	1	78	theme	mechanical	277:286	arg1	behaviors					288:296	unsatisfactory mechanical behaviors	262:296	unsatisfactory mechanical behaviors	262:296	Hydrogels are widely used in fields such as drug delivery, tissue regeneration, soft robotics and flexible smart electronic devices, yet their application is often limited by unsatisfactory mechanical behaviors.
34586150	3	79	theme	gum/gelatin	667:677	arg1	hydrogel					689:696	a virgin gellan gum/gelatin composite hydrogel	651:696	a virgin gellan gum/gelatin composite hydrogel	651:696	Here, a novel DN hydrogel composed fully of natural polymers exhibiting remarkable mechanical properties and conductivity is prepared by simply soaking a virgin gellan gum/gelatin composite hydrogel in a mixed solution of Na2SO4 and (NH4)2SO4.
34586150	5	80	theme	interaction	1178:1188	arg1	points					1203:1208	the electrostatic interaction crosslinking points	1160:1208	the electrostatic interaction crosslinking points introduced by Na+ in the gellan gum network	1160:1252	The improvement in the mechanical properties of the DN gel is attributed to the chain-entanglement crosslinking points introduced by SO42- in the gelatin network and the electrostatic interaction crosslinking points introduced by Na+ in the gellan gum network.
34586150	0	81	theme	conductive	29:38	arg1	hydrogel					77:84	An extremely tough and ionic conductive natural-polymer-based double network hydrogel	0:84	An extremely tough and ionic conductive natural-polymer-based double network hydrogel.	0:85	An extremely tough and ionic conductive natural-polymer-based double network hydrogel.
34586150	5	82	theme	DN	1046:1047	arg1	gel					1049:1051	the DN gel	1042:1051	the DN gel	1042:1051	The improvement in the mechanical properties of the DN gel is attributed to the chain-entanglement crosslinking points introduced by SO42- in the gelatin network and the electrostatic interaction crosslinking points introduced by Na+ in the gellan gum network.
34586150	7	83	theme	potential	1525:1533	arg1	applications					1535:1546	potential applications	1525:1546	potential applications in biomedical engineering and flexible electronic devices	1525:1604	The developed gellan gum/gelatin DN hydrogel has shown a new pathway towards strengthening natural-polymer-based DN hydrogels and towards potential applications in biomedical engineering and flexible electronic devices.
34586150	3	84	theme	virgin	653:658	arg1	hydrogel					689:696	a virgin gellan gum/gelatin composite hydrogel	651:696	a virgin gellan gum/gelatin composite hydrogel	651:696	Here, a novel DN hydrogel composed fully of natural polymers exhibiting remarkable mechanical properties and conductivity is prepared by simply soaking a virgin gellan gum/gelatin composite hydrogel in a mixed solution of Na2SO4 and (NH4)2SO4.
34586150	2	85	theme	impressive	408:417	arg1	properties					430:439	impressive mechanical properties	408:439	impressive mechanical properties	408:439	Among the various improvement strategies, double network (DN) hydrogels from synthetic polymers demonstrated impressive mechanical properties, while those from natural polymers were usually inferior.
34586150	0	86	theme	double	62:67	arg1	hydrogel					77:84	An extremely tough and ionic conductive natural-polymer-based double network hydrogel	0:84	An extremely tough and ionic conductive natural-polymer-based double network hydrogel.	0:85	An extremely tough and ionic conductive natural-polymer-based double network hydrogel.
34586150	4	87	theme	kJ	924:925	arg1	toughness					902:910	high fracture toughness	888:910	high fracture toughness (up to 27.7 kJ m-2)	888:930	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	4	87	theme	kJ	924:925	arg1	m-2					927:929	up to 27.7 kJ m-2	913:929	up to 27.7 kJ m-2	913:929	This hydrogel exhibits a tunable Young's modulus (0.08 to 42.6 MPa), good fracture stress (0.05 to 7.5 MPa), good fracture stretch (1.4 to 7.1), high fracture toughness (up to 27.7 kJ m-2), and high ionic conductivity (up to 11.4 S m-1 at f = 1 kHz).
34586150	2	88	theme	double	341:346	arg1	hydrogels					361:369	double network (DN) hydrogels	341:369	double network (DN) hydrogels from synthetic polymers	341:393	Among the various improvement strategies, double network (DN) hydrogels from synthetic polymers demonstrated impressive mechanical properties, while those from natural polymers were usually inferior.
34586150	5	89	theme	mechanical	1017:1026	arg1	properties					1028:1037	the mechanical properties	1013:1037	the mechanical properties of the DN gel	1013:1051	The improvement in the mechanical properties of the DN gel is attributed to the chain-entanglement crosslinking points introduced by SO42- in the gelatin network and the electrostatic interaction crosslinking points introduced by Na+ in the gellan gum network.
34586150	2	90	from	polymers	386:393	arg1	hydrogels					361:369	double network (DN) hydrogels	341:369	double network (DN) hydrogels from synthetic polymers	341:393	Among the various improvement strategies, double network (DN) hydrogels from synthetic polymers demonstrated impressive mechanical properties, while those from natural polymers were usually inferior.
34586150	6	91	theme	DN	1338:1339	arg1	gel					1341:1343	the DN gel	1334:1343	the DN gel	1334:1343	The high ionic conductivity of the DN gel is attributed to the infiltration of the DN gel in a salt solution of high concentration.
34586150	5	92	theme	chain-entanglement	1074:1091	arg1	points					1106:1111	the chain-entanglement crosslinking points	1070:1111	the chain-entanglement crosslinking points introduced by SO42- in the gelatin network and the electrostatic interaction crosslinking points introduced by Na+ in the gellan gum network	1070:1252	The improvement in the mechanical properties of the DN gel is attributed to the chain-entanglement crosslinking points introduced by SO42- in the gelatin network and the electrostatic interaction crosslinking points introduced by Na+ in the gellan gum network.
34586150	3	93	theme	Na2SO4	721:726	arg1	solution					709:716	a mixed solution	701:716	a mixed solution of Na2SO4 and (NH4)2SO4	701:740	Here, a novel DN hydrogel composed fully of natural polymers exhibiting remarkable mechanical properties and conductivity is prepared by simply soaking a virgin gellan gum/gelatin composite hydrogel in a mixed solution of Na2SO4 and (NH4)2SO4.
34586150	5	94	theme	gellan	1235:1240	arg1	network					1246:1252	the gellan gum network	1231:1252	the gellan gum network	1231:1252	The improvement in the mechanical properties of the DN gel is attributed to the chain-entanglement crosslinking points introduced by SO42- in the gelatin network and the electrostatic interaction crosslinking points introduced by Na+ in the gellan gum network.
32734660	1	0	theme	sponge	155:160	arg1	kind					94:97	A kind	92:97	A kind of commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature	92:283	A kind of commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature was fabricated for immune stimulation and modulation.
32734660	5	1	theme	intracellular	901:913	arg1	concentration					915:927	intracellular concentration	901:927	intracellular concentration	901:927	Results of particle size and intracellular concentration analyses suggested that the released carrageenan might enter into the cellular interior of RAW264.7 in the form of microgels or protein complexes.
32734660	5	2	theme	concentration	915:927	arg1	Results					872:878	Results	872:878	Results of particle size and intracellular concentration analyses	872:936	Results of particle size and intracellular concentration analyses suggested that the released carrageenan might enter into the cellular interior of RAW264.7 in the form of microgels or protein complexes.
32734660	1	3	from	temperature	273:283	arg1	turn					216:219	turn	216:219	turn	216:219	A kind of commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature was fabricated for immune stimulation and modulation.
32734660	3	4	theme	sponge	548:553	arg1	liquor					534:539	the leaching liquor	521:539	the leaching liquor of the sponge	521:553	RT-PCR and ELISA experiments showed that the leaching liquor of the sponge could stimulate RAW264.7 from M0 state to a polarized state by secreting more anti-inflammatory factor IL-10 than pro-inflammatory ones, such as, IL-6 and TNF-α.
32734660	1	5	contain	containing	162:171	arg2	ν-carrageenan					189:201	ν-carrageenan	189:201	ν-carrageenan	189:201	A kind of commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature was fabricated for immune stimulation and modulation.
32734660	1	5	contain	containing	162:171	arg2	κ-					173:174	κ-	173:174	κ-	173:174	A kind of commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature was fabricated for immune stimulation and modulation.
32734660	1	5	contain	containing	162:171	arg1	sponge					155:160	commercial hybrid carrageenan (HC)/agarose composite sponge	102:160	commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature	102:283	A kind of commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature was fabricated for immune stimulation and modulation.
32734660	1	5	contain	containing	162:171	arg2	ι-					181:182	ι-	181:182	ι-	181:182	A kind of commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature was fabricated for immune stimulation and modulation.
32734660	1	5	contain	containing	162:171	arg2	μ-					177:178	μ-	177:178	μ-	177:178	A kind of commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature was fabricated for immune stimulation and modulation.
32734660	4	6	theme	leaching	763:770	arg1	liquor					772:777	the leaching liquor	759:777	the leaching liquor	759:777	Transwell experiments also indicated that the leaching liquor could promote the proliferation of NIH-3T3 by stimulating RAW264.7 of M0 state after 7 days.
32734660	2	7	theme	Release	339:345	arg1	behavior					347:354	Release behavior	339:354	Release behavior	339:354	Release behavior demonstrated that the hybrid carrageenan contained sponge was mechanically stable and could release carrageenan constantly.
32734660	4	8	theme	Transwell	717:725	arg1	experiments					727:737	Transwell experiments	717:737	Transwell experiments	717:737	Transwell experiments also indicated that the leaching liquor could promote the proliferation of NIH-3T3 by stimulating RAW264.7 of M0 state after 7 days.
32734660	4	9	theme	state	852:856	arg1	RAW264.7					837:844	RAW264.7	837:844	RAW264.7 of M0 state after 7 days	837:869	Transwell experiments also indicated that the leaching liquor could promote the proliferation of NIH-3T3 by stimulating RAW264.7 of M0 state after 7 days.
32734660	5	10	theme	cellular	999:1006	arg1	interior					1008:1015	the cellular interior	995:1015	the cellular interior of RAW264.7	995:1027	Results of particle size and intracellular concentration analyses suggested that the released carrageenan might enter into the cellular interior of RAW264.7 in the form of microgels or protein complexes.
32734660	2	11	theme	carrageenan	385:395	arg1	sponge					407:412	the hybrid carrageenan contained sponge	374:412	the hybrid carrageenan contained sponge	374:412	Release behavior demonstrated that the hybrid carrageenan contained sponge was mechanically stable and could release carrageenan constantly.
32734660	2	11	theme	carrageenan	385:395	arg1	stable					431:436	stable	431:436	stable	431:436	Release behavior demonstrated that the hybrid carrageenan contained sponge was mechanically stable and could release carrageenan constantly.
32734660	4	12	theme	M0	849:850	arg1	state					852:856	M0 state	849:856	M0 state	849:856	Transwell experiments also indicated that the leaching liquor could promote the proliferation of NIH-3T3 by stimulating RAW264.7 of M0 state after 7 days.
32734660	6	13	theme	promising	1098:1106	arg1	candidate					1108:1116	a promising candidate	1096:1116	a promising candidate for skin wound dressing	1096:1140	The sponge would be a promising candidate for skin wound dressing.
32734660	6	13	theme	promising	1098:1106	arg1	sponge					1080:1085	The sponge	1076:1085	The sponge	1076:1085	The sponge would be a promising candidate for skin wound dressing.
32734660	2	14	theme	hybrid	378:383	arg1	sponge					407:412	the hybrid carrageenan contained sponge	374:412	the hybrid carrageenan contained sponge	374:412	Release behavior demonstrated that the hybrid carrageenan contained sponge was mechanically stable and could release carrageenan constantly.
32734660	2	14	theme	hybrid	378:383	arg1	stable					431:436	stable	431:436	stable	431:436	Release behavior demonstrated that the hybrid carrageenan contained sponge was mechanically stable and could release carrageenan constantly.
32734660	5	15	theme	released	957:964	arg1	carrageenan					966:976	the released carrageenan	953:976	the released carrageenan	953:976	Results of particle size and intracellular concentration analyses suggested that the released carrageenan might enter into the cellular interior of RAW264.7 in the form of microgels or protein complexes.
32734660	3	16	theme	M0	585:586	arg1	state					588:592	M0 state	585:592	M0 state	585:592	RT-PCR and ELISA experiments showed that the leaching liquor of the sponge could stimulate RAW264.7 from M0 state to a polarized state by secreting more anti-inflammatory factor IL-10 than pro-inflammatory ones, such as, IL-6 and TNF-α.
32734660	3	17	from	state	588:592	arg1	RAW264.7					571:578	RAW264.7	571:578	RAW264.7 from M0 state to a polarized state	571:613	RT-PCR and ELISA experiments showed that the leaching liquor of the sponge could stimulate RAW264.7 from M0 state to a polarized state by secreting more anti-inflammatory factor IL-10 than pro-inflammatory ones, such as, IL-6 and TNF-α.
32734660	5	18	theme	particle	883:890	arg1	size					892:895	particle size	883:895	particle size	883:895	Results of particle size and intracellular concentration analyses suggested that the released carrageenan might enter into the cellular interior of RAW264.7 in the form of microgels or protein complexes.
32734660	1	19	theme	commercial	102:111	arg1	sponge					155:160	commercial hybrid carrageenan (HC)/agarose composite sponge	102:160	commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature	102:283	A kind of commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature was fabricated for immune stimulation and modulation.
32734660	0	20	theme	composite	22:30	arg1	sponge					32:37	composite sponge	22:37	composite sponge	22:37	A carrageenan/agarose composite sponge and its immunomodulatory activities toward RAW264.7.
32734660	1	21	theme	human	262:266	arg1	temperature					273:283	human body temperature	262:283	human body temperature	262:283	A kind of commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature was fabricated for immune stimulation and modulation.
32734660	1	22	theme	hybrid	113:118	arg1	sponge					155:160	commercial hybrid carrageenan (HC)/agarose composite sponge	102:160	commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature	102:283	A kind of commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature was fabricated for immune stimulation and modulation.
32734660	3	23	theme	leaching	525:532	arg1	liquor					534:539	the leaching liquor	521:539	the leaching liquor of the sponge	521:553	RT-PCR and ELISA experiments showed that the leaching liquor of the sponge could stimulate RAW264.7 from M0 state to a polarized state by secreting more anti-inflammatory factor IL-10 than pro-inflammatory ones, such as, IL-6 and TNF-α.
32734660	1	24	theme	body	268:271	arg1	temperature					273:283	human body temperature	262:283	human body temperature	262:283	A kind of commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature was fabricated for immune stimulation and modulation.
32734660	3	25	theme	anti-inflammatory	633:649	arg1	IL-10					658:662	anti-inflammatory factor IL-10	633:662	more anti-inflammatory factor IL-10 than pro-inflammatory ones	628:689	RT-PCR and ELISA experiments showed that the leaching liquor of the sponge could stimulate RAW264.7 from M0 state to a polarized state by secreting more anti-inflammatory factor IL-10 than pro-inflammatory ones, such as, IL-6 and TNF-α.
32734660	3	26	theme	polarized	599:607	arg1	state					609:613	a polarized state	597:613	a polarized state	597:613	RT-PCR and ELISA experiments showed that the leaching liquor of the sponge could stimulate RAW264.7 from M0 state to a polarized state by secreting more anti-inflammatory factor IL-10 than pro-inflammatory ones, such as, IL-6 and TNF-α.
32734660	2	27	theme	contained	397:405	arg1	sponge					407:412	the hybrid carrageenan contained sponge	374:412	the hybrid carrageenan contained sponge	374:412	Release behavior demonstrated that the hybrid carrageenan contained sponge was mechanically stable and could release carrageenan constantly.
32734660	2	27	theme	contained	397:405	arg1	stable					431:436	stable	431:436	stable	431:436	Release behavior demonstrated that the hybrid carrageenan contained sponge was mechanically stable and could release carrageenan constantly.
32734660	5	28	theme	size	892:895	arg1	Results					872:878	Results	872:878	Results of particle size and intracellular concentration analyses	872:936	Results of particle size and intracellular concentration analyses suggested that the released carrageenan might enter into the cellular interior of RAW264.7 in the form of microgels or protein complexes.
32734660	3	29	theme	factor	651:656	arg1	IL-10					658:662	anti-inflammatory factor IL-10	633:662	more anti-inflammatory factor IL-10 than pro-inflammatory ones	628:689	RT-PCR and ELISA experiments showed that the leaching liquor of the sponge could stimulate RAW264.7 from M0 state to a polarized state by secreting more anti-inflammatory factor IL-10 than pro-inflammatory ones, such as, IL-6 and TNF-α.
32734660	5	30	theme	microgels	1044:1052	arg1	form					1036:1039	the form	1032:1039	the form of microgels or protein complexes	1032:1073	Results of particle size and intracellular concentration analyses suggested that the released carrageenan might enter into the cellular interior of RAW264.7 in the form of microgels or protein complexes.
32734660	6	31	theme	skin	1122:1125	arg1	dressing					1133:1140	skin wound dressing	1122:1140	skin wound dressing	1122:1140	The sponge would be a promising candidate for skin wound dressing.
32734660	1	32	theme	carrageenan	120:130	arg1	sponge					155:160	commercial hybrid carrageenan (HC)/agarose composite sponge	102:160	commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature	102:283	A kind of commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature was fabricated for immune stimulation and modulation.
32734660	0	33	theme	immunomodulatory	47:62	arg1	activities					64:73	its immunomodulatory activities	43:73	its immunomodulatory activities toward RAW264.7	43:89	A carrageenan/agarose composite sponge and its immunomodulatory activities toward RAW264.7.
32734660	5	34	theme	protein	1057:1063	arg1	complexes					1065:1073	protein complexes	1057:1073	protein complexes	1057:1073	Results of particle size and intracellular concentration analyses suggested that the released carrageenan might enter into the cellular interior of RAW264.7 in the form of microgels or protein complexes.
32734660	5	35	theme	complexes	1065:1073	arg1	form					1036:1039	the form	1032:1039	the form of microgels or protein complexes	1032:1073	Results of particle size and intracellular concentration analyses suggested that the released carrageenan might enter into the cellular interior of RAW264.7 in the form of microgels or protein complexes.
32734660	1	36	theme	HC	133:134	arg1	sponge					155:160	commercial hybrid carrageenan (HC)/agarose composite sponge	102:160	commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature	102:283	A kind of commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature was fabricated for immune stimulation and modulation.
32734660	5	37	dep	size	892:895	arg1	analyses					929:936	analyses	929:936	analyses	929:936	Results of particle size and intracellular concentration analyses suggested that the released carrageenan might enter into the cellular interior of RAW264.7 in the form of microgels or protein complexes.
32734660	3	38	theme	pro-inflammatory	669:684	arg1	ones					686:689	pro-inflammatory ones	669:689	pro-inflammatory ones	669:689	RT-PCR and ELISA experiments showed that the leaching liquor of the sponge could stimulate RAW264.7 from M0 state to a polarized state by secreting more anti-inflammatory factor IL-10 than pro-inflammatory ones, such as, IL-6 and TNF-α.
32734660	5	39	theme	RAW264.7	1020:1027	arg1	interior					1008:1015	the cellular interior	995:1015	the cellular interior of RAW264.7	995:1027	Results of particle size and intracellular concentration analyses suggested that the released carrageenan might enter into the cellular interior of RAW264.7 in the form of microgels or protein complexes.
32734660	3	40	theme	ELISA	491:495	arg1	experiments					497:507	ELISA experiments	491:507	ELISA experiments	491:507	RT-PCR and ELISA experiments showed that the leaching liquor of the sponge could stimulate RAW264.7 from M0 state to a polarized state by secreting more anti-inflammatory factor IL-10 than pro-inflammatory ones, such as, IL-6 and TNF-α.
32734660	1	41	dep	hydrogel	226:233	arg1	carrageenan					247:257	carrageenan	247:257	carrageenan	247:257	A kind of commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature was fabricated for immune stimulation and modulation.
32734660	4	42	theme	NIH-3T3	814:820	arg1	proliferation					797:809	the proliferation	793:809	the proliferation of NIH-3T3	793:820	Transwell experiments also indicated that the leaching liquor could promote the proliferation of NIH-3T3 by stimulating RAW264.7 of M0 state after 7 days.
32734660	1	43	theme	/agarose	136:143	arg1	sponge					155:160	commercial hybrid carrageenan (HC)/agarose composite sponge	102:160	commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature	102:283	A kind of commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature was fabricated for immune stimulation and modulation.
32734660	1	44	theme	immune	304:309	arg1	stimulation					311:321	immune stimulation	304:321	immune stimulation	304:321	A kind of commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature was fabricated for immune stimulation and modulation.
32734660	6	45	theme	wound	1127:1131	arg1	dressing					1133:1140	skin wound dressing	1122:1140	skin wound dressing	1122:1140	The sponge would be a promising candidate for skin wound dressing.
32734660	1	46	theme	composite	145:153	arg1	sponge					155:160	commercial hybrid carrageenan (HC)/agarose composite sponge	102:160	commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature	102:283	A kind of commercial hybrid carrageenan (HC)/agarose composite sponge containing κ-, μ-, ι-, and ν-carrageenan, which could turn into hydrogel and release carrageenan at human body temperature was fabricated for immune stimulation and modulation.
32450325	10	0	theme	CMPC	1112:1115	arg1	specimens					1117:1125	CMPC specimens	1112:1125	CMPC specimens	1112:1125	Osteoblasts (MC3T3-E1) showed significantly greater adherence, proliferation, and differentiation on CMPC specimens than on MPC.
32450325	11	1	theme	ERK	1273:1275	arg1	phosphorylation					1277:1291	enhanced FAK and ERK phosphorylation	1256:1291	phosphorylation	1277:1291	Finally, CMPCs effectively increased the adsorption of fibronectin and activated integrin signaling as indicated by enhanced FAK and ERK phosphorylation.
32450325	9	2	theme	Mg2+	971:974	arg1	release					976:982	Mg2+ release	971:982	Mg2+ release	971:982	Furthermore, CMPC samples showed similar degradability and Mg2+ release to MPC in Tris-HCl buffer.
32450325	0	3	theme	bone	92:95	arg1	regeneration					97:108	bone regeneration	92:108	bone regeneration	92:108	An injectable bioactive magnesium phosphate cement incorporating carboxymethyl chitosan for bone regeneration.
32450325	4	4	theme	MC3T3-E1	498:505	arg1	cells					507:511	pre-osteoblast MC3T3-E1 cells	483:511	pre-osteoblast MC3T3-E1 cells	483:511	The in vitro cellular responses of pre-osteoblast MC3T3-E1 cells to CMPCs including adhesion, proliferation, and differentiation were quantified and the underlying cellular mechanisms investigated.
32450325	5	5	theme	longer	656:661	arg1	times					671:675	longer setting times	656:675	longer setting times	656:675	CMPCs had longer setting times and lower setting temperatures.
32450325	7	6	theme	%	786:786	arg1	CMC					788:790	5% CMC	785:790	5% CMC	785:790	The CMPC containing 5% CMC had the highest compressive strength and washout resistance.
32450325	9	7	theme	CMPC	925:928	arg1	samples					930:936	CMPC samples	925:936	CMPC samples	925:936	Furthermore, CMPC samples showed similar degradability and Mg2+ release to MPC in Tris-HCl buffer.
32450325	4	8	theme	pre-osteoblast	483:496	arg1	cells					507:511	pre-osteoblast MC3T3-E1 cells	483:511	pre-osteoblast MC3T3-E1 cells	483:511	The in vitro cellular responses of pre-osteoblast MC3T3-E1 cells to CMPCs including adhesion, proliferation, and differentiation were quantified and the underlying cellular mechanisms investigated.
32450325	5	9	theme	setting	663:669	arg1	times					671:675	longer setting times	656:675	longer setting times	656:675	CMPCs had longer setting times and lower setting temperatures.
32450325	7	10	contain	containing	774:783	arg1	CMPC					769:772	The CMPC	765:772	The CMPC containing 5% CMC	765:790	The CMPC containing 5% CMC had the highest compressive strength and washout resistance.
32450325	7	10	contain	containing	774:783	arg2	CMC					788:790	5% CMC	785:790	5% CMC	785:790	The CMPC containing 5% CMC had the highest compressive strength and washout resistance.
32450325	7	11	contain	had	792:794	arg1	CMPC					769:772	The CMPC	765:772	The CMPC containing 5% CMC	765:790	The CMPC containing 5% CMC had the highest compressive strength and washout resistance.
32450325	7	11	contain	had	792:794	arg2	resistance					841:850	washout resistance	833:850	washout resistance	833:850	The CMPC containing 5% CMC had the highest compressive strength and washout resistance.
32450325	7	11	contain	had	792:794	arg2	strength					820:827	compressive strength	808:827	compressive strength	808:827	The CMPC containing 5% CMC had the highest compressive strength and washout resistance.
32450325	4	12	theme	in	452:453	arg1	responses					470:478	The in vitro cellular responses	448:478	The in vitro cellular responses of pre-osteoblast MC3T3-E1 cells to CMPCs including adhesion, proliferation, and differentiation	448:575	The in vitro cellular responses of pre-osteoblast MC3T3-E1 cells to CMPCs including adhesion, proliferation, and differentiation were quantified and the underlying cellular mechanisms investigated.
32450325	4	13	theme	cellular	461:468	arg1	responses					470:478	The in vitro cellular responses	448:478	The in vitro cellular responses of pre-osteoblast MC3T3-E1 cells to CMPCs including adhesion, proliferation, and differentiation	448:575	The in vitro cellular responses of pre-osteoblast MC3T3-E1 cells to CMPCs including adhesion, proliferation, and differentiation were quantified and the underlying cellular mechanisms investigated.
32450325	12	14	theme	bone	1423:1426	arg1	regeneration					1428:1439	bone regeneration	1423:1439	bone regeneration	1423:1439	Our novel CMPC composites have improved physicochemical properties and cellular responses and represent a promising material for bone regeneration.
32450325	6	15	theme	CMPC	709:712	arg1	injectability					714:726	CMPC injectability	709:726	CMPC injectability	709:726	CMPC injectability was enhanced by the addition of CMC.
32450325	1	16	theme	in	171:172	arg1	scaffold					179:186	an in situ scaffold	168:186	an in situ scaffold to repair bone defects	168:209	Magnesium phosphate cement (MPC) can be injected to form an in situ scaffold to repair bone defects.
32450325	0	17	theme	injectable	3:12	arg1	cement					44:49	An injectable bioactive magnesium phosphate cement	0:49	An injectable bioactive magnesium phosphate cement	0:49	An injectable bioactive magnesium phosphate cement incorporating carboxymethyl chitosan for bone regeneration.
32450325	2	18	dep	chitosan	326:333	arg1	CMC					336:338	CMC	336:338	CMC	336:338	Here we synthesized novel injectable bioactive cements (CMPCs) by incorporating different ratios of carboxymethyl chitosan (CMC, 0-10%) into MPC.
32450325	2	18	dep	chitosan	326:333	arg1	%					345:345	0-10%	341:345	0-10%	341:345	Here we synthesized novel injectable bioactive cements (CMPCs) by incorporating different ratios of carboxymethyl chitosan (CMC, 0-10%) into MPC.
32450325	8	19	theme	neutral	870:876	arg1	pH					878:879	a more neutral pH	863:879	a more neutral pH	863:879	CMPCs had a more neutral pH compared to MPC at four weeks.
32450325	12	20	theme	CMPC	1304:1307	arg1	composites					1309:1318	Our novel CMPC composites	1294:1318	Our novel CMPC composites	1294:1318	Our novel CMPC composites have improved physicochemical properties and cellular responses and represent a promising material for bone regeneration.
32450325	10	21	theme	greater	1055:1061	arg1	adherence					1063:1071	adherence	1063:1071	adherence	1063:1071	Osteoblasts (MC3T3-E1) showed significantly greater adherence, proliferation, and differentiation on CMPC specimens than on MPC.
32450325	1	22	theme	Magnesium	111:119	arg1	MPC					139:141	MPC	139:141	MPC	139:141	Magnesium phosphate cement (MPC) can be injected to form an in situ scaffold to repair bone defects.
32450325	1	22	theme	Magnesium	111:119	arg1	cement					131:136	Magnesium phosphate cement	111:136	Magnesium phosphate cement (MPC)	111:142	Magnesium phosphate cement (MPC) can be injected to form an in situ scaffold to repair bone defects.
32450325	2	23	dep	bioactive	249:257	arg1	cements					259:265	cements	259:265	cements	259:265	Here we synthesized novel injectable bioactive cements (CMPCs) by incorporating different ratios of carboxymethyl chitosan (CMC, 0-10%) into MPC.
32450325	0	24	theme	magnesium	24:32	arg1	cement					44:49	An injectable bioactive magnesium phosphate cement	0:49	An injectable bioactive magnesium phosphate cement	0:49	An injectable bioactive magnesium phosphate cement incorporating carboxymethyl chitosan for bone regeneration.
32450325	7	25	theme	highest	800:806	arg1	strength					820:827	compressive strength	808:827	compressive strength	808:827	The CMPC containing 5% CMC had the highest compressive strength and washout resistance.
32450325	11	26	theme	integrin	1221:1228	arg1	signaling					1230:1238	integrin signaling	1221:1238	integrin signaling	1221:1238	Finally, CMPCs effectively increased the adsorption of fibronectin and activated integrin signaling as indicated by enhanced FAK and ERK phosphorylation.
32450325	12	27	theme	cellular	1365:1372	arg1	responses					1374:1382	cellular responses	1365:1382	cellular responses	1365:1382	Our novel CMPC composites have improved physicochemical properties and cellular responses and represent a promising material for bone regeneration.
32450325	1	28	theme	phosphate	121:129	arg1	MPC					139:141	MPC	139:141	MPC	139:141	Magnesium phosphate cement (MPC) can be injected to form an in situ scaffold to repair bone defects.
32450325	1	28	theme	phosphate	121:129	arg1	cement					131:136	Magnesium phosphate cement	111:136	Magnesium phosphate cement (MPC)	111:142	Magnesium phosphate cement (MPC) can be injected to form an in situ scaffold to repair bone defects.
32450325	7	29	theme	washout	833:839	arg1	resistance					841:850	washout resistance	833:850	washout resistance	833:850	The CMPC containing 5% CMC had the highest compressive strength and washout resistance.
32450325	0	30	theme	bioactive	14:22	arg1	cement					44:49	An injectable bioactive magnesium phosphate cement	0:49	An injectable bioactive magnesium phosphate cement	0:49	An injectable bioactive magnesium phosphate cement incorporating carboxymethyl chitosan for bone regeneration.
32450325	4	31	dep	in	452:453	arg1	vitro					455:459	vitro	455:459	vitro	455:459	The in vitro cellular responses of pre-osteoblast MC3T3-E1 cells to CMPCs including adhesion, proliferation, and differentiation were quantified and the underlying cellular mechanisms investigated.
32450325	7	32	theme	compressive	808:818	arg1	strength					820:827	compressive strength	808:827	compressive strength	808:827	The CMPC containing 5% CMC had the highest compressive strength and washout resistance.
32450325	1	33	theme	repair	191:196	arg1	defects					203:209	repair bone defects	191:209	repair bone defects	191:209	Magnesium phosphate cement (MPC) can be injected to form an in situ scaffold to repair bone defects.
32450325	1	34	theme	bone	198:201	arg1	defects					203:209	repair bone defects	191:209	repair bone defects	191:209	Magnesium phosphate cement (MPC) can be injected to form an in situ scaffold to repair bone defects.
32450325	11	35	theme	enhanced	1256:1263	arg1	FAK					1265:1267	enhanced FAK and ERK phosphorylation	1256:1291	FAK	1265:1267	Finally, CMPCs effectively increased the adsorption of fibronectin and activated integrin signaling as indicated by enhanced FAK and ERK phosphorylation.
32450325	12	36	theme	novel	1298:1302	arg1	composites					1309:1318	Our novel CMPC composites	1294:1318	Our novel CMPC composites	1294:1318	Our novel CMPC composites have improved physicochemical properties and cellular responses and represent a promising material for bone regeneration.
32450325	3	37	theme	CMPCs	426:430	arg1	microstructures					407:421	microstructures	407:421	microstructures	407:421	The physiochemical properties, compositions, and microstructures of CMPCs were evaluated.
32450325	3	37	theme	CMPCs	426:430	arg1	properties					377:386	The physiochemical properties	358:386	The physiochemical properties	358:386	The physiochemical properties, compositions, and microstructures of CMPCs were evaluated.
32450325	3	37	theme	CMPCs	426:430	arg1	compositions					389:400	compositions	389:400	compositions	389:400	The physiochemical properties, compositions, and microstructures of CMPCs were evaluated.
32450325	0	38	theme	phosphate	34:42	arg1	cement					44:49	An injectable bioactive magnesium phosphate cement	0:49	An injectable bioactive magnesium phosphate cement	0:49	An injectable bioactive magnesium phosphate cement incorporating carboxymethyl chitosan for bone regeneration.
32450325	7	39	theme	5	785:785	arg1	%					786:786	%	786:786	%	786:786	The CMPC containing 5% CMC had the highest compressive strength and washout resistance.
32450325	12	40	theme	promising	1400:1408	arg1	material					1410:1417	a promising material	1398:1417	a promising material for bone regeneration	1398:1439	Our novel CMPC composites have improved physicochemical properties and cellular responses and represent a promising material for bone regeneration.
32450325	0	41	theme	carboxymethyl	65:77	arg1	chitosan					79:86	carboxymethyl chitosan	65:86	carboxymethyl chitosan for bone regeneration	65:108	An injectable bioactive magnesium phosphate cement incorporating carboxymethyl chitosan for bone regeneration.
32450325	4	42	theme	cells	507:511	arg1	responses					470:478	The in vitro cellular responses	448:478	The in vitro cellular responses of pre-osteoblast MC3T3-E1 cells to CMPCs including adhesion, proliferation, and differentiation	448:575	The in vitro cellular responses of pre-osteoblast MC3T3-E1 cells to CMPCs including adhesion, proliferation, and differentiation were quantified and the underlying cellular mechanisms investigated.
32450325	8	43	contain	had	859:861	arg2	pH					878:879	a more neutral pH	863:879	a more neutral pH	863:879	CMPCs had a more neutral pH compared to MPC at four weeks.
32450325	8	43	contain	had	859:861	arg1	CMPCs					853:857	CMPCs	853:857	CMPCs	853:857	CMPCs had a more neutral pH compared to MPC at four weeks.
32450325	12	44	contain	have	1320:1323	arg2	responses					1374:1382	cellular responses	1365:1382	cellular responses	1365:1382	Our novel CMPC composites have improved physicochemical properties and cellular responses and represent a promising material for bone regeneration.
32450325	12	44	contain	have	1320:1323	arg1	composites					1309:1318	Our novel CMPC composites	1294:1318	Our novel CMPC composites	1294:1318	Our novel CMPC composites have improved physicochemical properties and cellular responses and represent a promising material for bone regeneration.
32450325	12	44	contain	have	1320:1323	arg2	properties					1350:1359	improved physicochemical properties	1325:1359	improved physicochemical properties	1325:1359	Our novel CMPC composites have improved physicochemical properties and cellular responses and represent a promising material for bone regeneration.
32450325	5	45	theme	lower	681:685	arg1	temperatures					695:706	lower setting temperatures	681:706	lower setting temperatures	681:706	CMPCs had longer setting times and lower setting temperatures.
32450325	1	46	dep	in	171:172	arg1	situ					174:177	situ	174:177	situ	174:177	Magnesium phosphate cement (MPC) can be injected to form an in situ scaffold to repair bone defects.
32450325	6	47	theme	CMC	760:762	arg1	addition					748:755	the addition	744:755	the addition of CMC	744:762	CMPC injectability was enhanced by the addition of CMC.
32450325	9	48	theme	Tris-HCl	994:1001	arg1	buffer					1003:1008	Tris-HCl buffer	994:1008	Tris-HCl buffer	994:1008	Furthermore, CMPC samples showed similar degradability and Mg2+ release to MPC in Tris-HCl buffer.
32450325	12	49	theme	improved	1325:1332	arg1	properties					1350:1359	improved physicochemical properties	1325:1359	improved physicochemical properties	1325:1359	Our novel CMPC composites have improved physicochemical properties and cellular responses and represent a promising material for bone regeneration.
32450325	5	50	theme	setting	687:693	arg1	temperatures					695:706	lower setting temperatures	681:706	lower setting temperatures	681:706	CMPCs had longer setting times and lower setting temperatures.
32450325	2	51	theme	chitosan	326:333	arg1	ratios					302:307	different ratios	292:307	different ratios of carboxymethyl chitosan (CMC, 0-10%)	292:346	Here we synthesized novel injectable bioactive cements (CMPCs) by incorporating different ratios of carboxymethyl chitosan (CMC, 0-10%) into MPC.
32450325	2	52	theme	different	292:300	arg1	ratios					302:307	different ratios	292:307	different ratios of carboxymethyl chitosan (CMC, 0-10%)	292:346	Here we synthesized novel injectable bioactive cements (CMPCs) by incorporating different ratios of carboxymethyl chitosan (CMC, 0-10%) into MPC.
32450325	2	53	theme	carboxymethyl	312:324	arg1	chitosan					326:333	carboxymethyl chitosan	312:333	carboxymethyl chitosan (CMC, 0-10%)	312:346	Here we synthesized novel injectable bioactive cements (CMPCs) by incorporating different ratios of carboxymethyl chitosan (CMC, 0-10%) into MPC.
32450325	11	54	theme	fibronectin	1195:1205	arg1	adsorption					1181:1190	the adsorption	1177:1190	the adsorption of fibronectin	1177:1205	Finally, CMPCs effectively increased the adsorption of fibronectin and activated integrin signaling as indicated by enhanced FAK and ERK phosphorylation.
32450325	12	55	theme	physicochemical	1334:1348	arg1	properties					1350:1359	improved physicochemical properties	1325:1359	improved physicochemical properties	1325:1359	Our novel CMPC composites have improved physicochemical properties and cellular responses and represent a promising material for bone regeneration.
32450325	4	56	theme	cellular	612:619	arg1	mechanisms					621:630	the underlying cellular mechanisms	597:630	the underlying cellular mechanisms	597:630	The in vitro cellular responses of pre-osteoblast MC3T3-E1 cells to CMPCs including adhesion, proliferation, and differentiation were quantified and the underlying cellular mechanisms investigated.
32450325	3	57	theme	physiochemical	362:375	arg1	properties					377:386	The physiochemical properties	358:386	The physiochemical properties	358:386	The physiochemical properties, compositions, and microstructures of CMPCs were evaluated.
32450325	5	58	contain	had	652:654	arg2	times					671:675	longer setting times	656:675	longer setting times	656:675	CMPCs had longer setting times and lower setting temperatures.
32450325	5	58	contain	had	652:654	arg1	CMPCs					646:650	CMPCs	646:650	CMPCs	646:650	CMPCs had longer setting times and lower setting temperatures.
32450325	5	58	contain	had	652:654	arg2	temperatures					695:706	lower setting temperatures	681:706	lower setting temperatures	681:706	CMPCs had longer setting times and lower setting temperatures.
32450325	9	59	theme	similar	945:951	arg1	degradability					953:965	similar degradability	945:965	similar degradability	945:965	Furthermore, CMPC samples showed similar degradability and Mg2+ release to MPC in Tris-HCl buffer.
32450325	4	60	theme	underlying	601:610	arg1	mechanisms					621:630	the underlying cellular mechanisms	597:630	the underlying cellular mechanisms	597:630	The in vitro cellular responses of pre-osteoblast MC3T3-E1 cells to CMPCs including adhesion, proliferation, and differentiation were quantified and the underlying cellular mechanisms investigated.
32441664	0	0	theme	external	89:96	arg1	data					106:109	international external quality data	75:109	international external quality data	75:109	Quality performance for indirect Xa inhibitor monitoring in patients using international external quality data.
32441664	5	1	theme	danaparoid	745:754	arg1	heparin					676:682	unfractionated heparin	661:682	unfractionated heparin (UFH)	661:688	Methods Laboratory tests results were evaluated for samples spiked with unfractionated heparin (UFH), low-molecular-weight-heparin (LMWH), fondaparinux and danaparoid sodium.
32441664	5	1	theme	danaparoid	745:754	arg1	sodium					756:761	fondaparinux and danaparoid sodium	728:761	sodium	756:761	Methods Laboratory tests results were evaluated for samples spiked with unfractionated heparin (UFH), low-molecular-weight-heparin (LMWH), fondaparinux and danaparoid sodium.
32441664	9	2	theme	method	1141:1146	arg1	composition					1148:1158	method composition	1141:1158	method composition	1141:1158	These differences may be caused by differences in method composition, such as the addition of dextran sulphate.
32441664	13	3	theme	therapeutic	1693:1703	arg1	ranges					1705:1710	therapeutic ranges	1693:1710	therapeutic ranges	1693:1710	The variation in measurements may have an effect on clinical implications, such as therapeutic ranges.
32441664	10	4	theme	anti-FXa	1244:1251	arg1	monitoring					1253:1262	anti-FXa monitoring	1244:1262	anti-FXa monitoring	1244:1262	Substantial interlaboratory variation in anti-FXa monitoring was observed for all parameters, particularly at low concentrations.
32441664	0	5	theme	international	75:87	arg1	data					106:109	international external quality data	75:109	international external quality data	75:109	Quality performance for indirect Xa inhibitor monitoring in patients using international external quality data.
32441664	0	6	from	performance	8:18	arg1	patients					60:67	patients	60:67	patients	60:67	Quality performance for indirect Xa inhibitor monitoring in patients using international external quality data.
32441664	2	7	theme	reagents	314:321	arg1	choice					304:309	the choice	300:309	the choice of reagents	300:321	However, anti-FXa has been shown to vary according to the choice of reagents.
32441664	7	8	theme	significant	923:933	arg1	differences					935:945	significant differences	923:945	significant differences in measured values between the frequently used methods (ANOVA: p < 0.001)	923:1019	Results Comparison of the results for all methods showed significant differences in measured values between the frequently used methods (ANOVA: p < 0.001).
32441664	0	9	theme	quality	98:104	arg1	data					106:109	international external quality data	75:109	international external quality data	75:109	Quality performance for indirect Xa inhibitor monitoring in patients using international external quality data.
32441664	6	10	theme	EQA	793:795	arg1	data					798:801	External quality assessment (EQA) data	764:801	External quality assessment (EQA) data from multiple years	764:821	External quality assessment (EQA) data from multiple years were used from more than 100 laboratories.
32441664	14	11	theme	anti-Xa	1853:1859	arg1	results					1861:1867	the anti-Xa results	1849:1867	the anti-Xa results	1849:1867	Furthermore, the longitudinal EQA data demonstrated a constant performance and, in at least 50% of the cases, improvement in the CV% of the anti-Xa results over time.
32441664	5	12	theme	Laboratory	597:606	arg1	tests					608:612	Laboratory tests	597:612	Methods Laboratory tests results	589:620	Methods Laboratory tests results were evaluated for samples spiked with unfractionated heparin (UFH), low-molecular-weight-heparin (LMWH), fondaparinux and danaparoid sodium.
32441664	3	13	theme	clinical	439:446	arg1	applications					448:459	the clinical applications	435:459	the clinical applications	435:459	In the present study, the performance of anti-FXa measurement was evaluated in order to gain more insight into the clinical applications.
32441664	13	14	contain	have	1644:1647	arg1	variation					1614:1622	The variation	1610:1622	The variation in measurements	1610:1638	The variation in measurements may have an effect on clinical implications, such as therapeutic ranges.
32441664	13	14	contain	have	1644:1647	arg2	effect					1652:1657	an effect	1649:1657	an effect	1649:1657	The variation in measurements may have an effect on clinical implications, such as therapeutic ranges.
32441664	3	15	theme	present	331:337	arg1	study					339:343	the present study	327:343	the present study	327:343	In the present study, the performance of anti-FXa measurement was evaluated in order to gain more insight into the clinical applications.
32441664	12	16	dep	UFH	1584:1586	arg1	measurement					1597:1607	measurement	1597:1607	measurement	1597:1607	Conclusions Our study demonstrates that the choice of the anti-FXa method is particularly important for UFH and LMWH measurement.
32441664	6	17	used	used	828:831	arg2	data					798:801	External quality assessment (EQA) data	764:801	External quality assessment (EQA) data from multiple years	764:821	External quality assessment (EQA) data from multiple years were used from more than 100 laboratories.
32441664	6	18	theme	assessment	781:790	arg1	data					798:801	External quality assessment (EQA) data	764:801	External quality assessment (EQA) data from multiple years	764:821	External quality assessment (EQA) data from multiple years were used from more than 100 laboratories.
32441664	0	19	theme	Quality	0:6	arg1	performance					8:18	Quality performance	0:18	Quality performance for indirect Xa inhibitor monitoring in patients	0:67	Quality performance for indirect Xa inhibitor monitoring in patients using international external quality data.
32441664	3	20	theme	more	417:420	arg1	insight					422:428	more insight	417:428	more insight into the clinical applications	417:459	In the present study, the performance of anti-FXa measurement was evaluated in order to gain more insight into the clinical applications.
32441664	14	21	theme	longitudinal	1730:1741	arg1	data					1747:1750	the longitudinal EQA data	1726:1750	the longitudinal EQA data	1726:1750	Furthermore, the longitudinal EQA data demonstrated a constant performance and, in at least 50% of the cases, improvement in the CV% of the anti-Xa results over time.
32441664	10	22	located	observed	1268:1275	arg1	concentrations					1317:1330	low concentrations	1313:1330	low concentrations	1313:1330	Substantial interlaboratory variation in anti-FXa monitoring was observed for all parameters, particularly at low concentrations.
32441664	10	22	located	observed	1268:1275	arg2	variation					1231:1239	Substantial interlaboratory variation	1203:1239	Substantial interlaboratory variation in anti-FXa monitoring	1203:1262	Substantial interlaboratory variation in anti-FXa monitoring was observed for all parameters, particularly at low concentrations.
32441664	10	23	theme	Substantial	1203:1213	arg1	variation					1231:1239	Substantial interlaboratory variation	1203:1239	Substantial interlaboratory variation in anti-FXa monitoring	1203:1262	Substantial interlaboratory variation in anti-FXa monitoring was observed for all parameters, particularly at low concentrations.
32441664	6	24	theme	quality	773:779	arg1	data					798:801	External quality assessment (EQA) data	764:801	External quality assessment (EQA) data from multiple years	764:821	External quality assessment (EQA) data from multiple years were used from more than 100 laboratories.
32441664	7	25	dep	ANOVA	1003:1007	arg1	p < 0.001					1010:1018	p < 0.001	1010:1018	ANOVA: p < 0.001	1003:1018	Results Comparison of the results for all methods showed significant differences in measured values between the frequently used methods (ANOVA: p < 0.001).
32441664	3	26	theme	anti-FXa	365:372	arg1	measurement					374:384	anti-FXa measurement	365:384	anti-FXa measurement	365:384	In the present study, the performance of anti-FXa measurement was evaluated in order to gain more insight into the clinical applications.
32441664	13	27	theme	clinical	1662:1669	arg1	ranges					1705:1710	therapeutic ranges	1693:1710	therapeutic ranges	1693:1710	The variation in measurements may have an effect on clinical implications, such as therapeutic ranges.
32441664	13	27	theme	clinical	1662:1669	arg1	implications					1671:1682	clinical implications	1662:1682	clinical implications	1662:1682	The variation in measurements may have an effect on clinical implications, such as therapeutic ranges.
32441664	6	28	theme	External	764:771	arg1	data					798:801	External quality assessment (EQA) data	764:801	External quality assessment (EQA) data from multiple years	764:821	External quality assessment (EQA) data from multiple years were used from more than 100 laboratories.
32441664	8	29	theme	largest	1026:1032	arg1	differences					1034:1044	The largest differences	1022:1044	The largest differences	1022:1044	The largest differences were observed for LMWH and UFH measurements.
32441664	9	30	from	differences	1126:1136	arg1	composition					1148:1158	method composition	1141:1158	method composition	1141:1158	These differences may be caused by differences in method composition, such as the addition of dextran sulphate.
32441664	0	31	theme	indirect	24:31	arg1	monitoring					46:55	indirect Xa inhibitor monitoring	24:55	indirect Xa inhibitor monitoring	24:55	Quality performance for indirect Xa inhibitor monitoring in patients using international external quality data.
32441664	7	32	dep	Results	866:872	arg1	showed					916:921	showed	916:921	showed significant differences in measured values between the frequently used methods (ANOVA: p < 0.001)	916:1019	Results Comparison of the results for all methods showed significant differences in measured values between the frequently used methods (ANOVA: p < 0.001).
32441664	14	33	from	improvement	1823:1833	arg1	results					1861:1867	the anti-Xa results	1849:1867	the anti-Xa results	1849:1867	Furthermore, the longitudinal EQA data demonstrated a constant performance and, in at least 50% of the cases, improvement in the CV% of the anti-Xa results over time.
32441664	14	33	from	improvement	1823:1833	arg1	%					1844:1844	the CV%	1838:1844	the CV% of the anti-Xa results	1838:1867	Furthermore, the longitudinal EQA data demonstrated a constant performance and, in at least 50% of the cases, improvement in the CV% of the anti-Xa results over time.
32441664	1	34	theme	gold	191:194	arg1	"					204:204	the "gold standard"	186:204	the "gold standard" for monitoring indirect anticoagulants	186:243	Objectives Chromogenic anti-activated factor X (FXa) assays are currently the "gold standard" for monitoring indirect anticoagulants.
32441664	1	34	theme	gold	191:194	arg1	assays					165:170	Objectives Chromogenic anti-activated factor X (FXa) assays	112:170	Objectives Chromogenic anti-activated factor X (FXa) assays	112:170	Objectives Chromogenic anti-activated factor X (FXa) assays are currently the "gold standard" for monitoring indirect anticoagulants.
32441664	10	35	from	variation	1231:1239	arg1	monitoring					1253:1262	anti-FXa monitoring	1244:1262	anti-FXa monitoring	1244:1262	Substantial interlaboratory variation in anti-FXa monitoring was observed for all parameters, particularly at low concentrations.
32441664	9	36	theme	sulphate	1193:1200	arg1	addition					1173:1180	the addition	1169:1180	the addition of dextran sulphate	1169:1200	These differences may be caused by differences in method composition, such as the addition of dextran sulphate.
32441664	1	37	theme	Objectives	112:121	arg1	"					204:204	the "gold standard"	186:204	the "gold standard" for monitoring indirect anticoagulants	186:243	Objectives Chromogenic anti-activated factor X (FXa) assays are currently the "gold standard" for monitoring indirect anticoagulants.
32441664	1	37	theme	Objectives	112:121	arg1	assays					165:170	Objectives Chromogenic anti-activated factor X (FXa) assays	112:170	Objectives Chromogenic anti-activated factor X (FXa) assays	112:170	Objectives Chromogenic anti-activated factor X (FXa) assays are currently the "gold standard" for monitoring indirect anticoagulants.
32441664	1	38	theme	standard	196:203	arg1	"					204:204	the "gold standard"	186:204	the "gold standard" for monitoring indirect anticoagulants	186:243	Objectives Chromogenic anti-activated factor X (FXa) assays are currently the "gold standard" for monitoring indirect anticoagulants.
32441664	1	38	theme	standard	196:203	arg1	assays					165:170	Objectives Chromogenic anti-activated factor X (FXa) assays	112:170	Objectives Chromogenic anti-activated factor X (FXa) assays	112:170	Objectives Chromogenic anti-activated factor X (FXa) assays are currently the "gold standard" for monitoring indirect anticoagulants.
32441664	0	39	theme	inhibitor	36:44	arg1	monitoring					46:55	indirect Xa inhibitor monitoring	24:55	indirect Xa inhibitor monitoring	24:55	Quality performance for indirect Xa inhibitor monitoring in patients using international external quality data.
32441664	14	40	theme	cases	1816:1820	arg1	%					1807:1807	at least 50%	1796:1807	at least 50% of the cases	1796:1820	Furthermore, the longitudinal EQA data demonstrated a constant performance and, in at least 50% of the cases, improvement in the CV% of the anti-Xa results over time.
32441664	14	40	theme	cases	1816:1820	arg1	cases					1816:1820	the cases	1812:1820	the cases	1812:1820	Furthermore, the longitudinal EQA data demonstrated a constant performance and, in at least 50% of the cases, improvement in the CV% of the anti-Xa results over time.
32441664	0	41	theme	Xa	33:34	arg1	monitoring					46:55	indirect Xa inhibitor monitoring	24:55	indirect Xa inhibitor monitoring	24:55	Quality performance for indirect Xa inhibitor monitoring in patients using international external quality data.
32441664	14	42	theme	constant	1767:1774	arg1	performance					1776:1786	a constant performance	1765:1786	a constant performance	1765:1786	Furthermore, the longitudinal EQA data demonstrated a constant performance and, in at least 50% of the cases, improvement in the CV% of the anti-Xa results over time.
32441664	8	43	dep	LMWH	1064:1067	arg1	measurements					1077:1088	measurements	1077:1088	measurements	1077:1088	The largest differences were observed for LMWH and UFH measurements.
32441664	12	44	theme	anti-FXa	1538:1545	arg1	method					1547:1552	the anti-FXa method	1534:1552	the anti-FXa method	1534:1552	Conclusions Our study demonstrates that the choice of the anti-FXa method is particularly important for UFH and LMWH measurement.
32441664	12	45	dep	Conclusions	1480:1490	arg1	demonstrates					1502:1513	demonstrates	1502:1513	demonstrates that the choice of the anti-FXa method is particularly important for UFH and LMWH measurement	1502:1607	Conclusions Our study demonstrates that the choice of the anti-FXa method is particularly important for UFH and LMWH measurement.
32441664	13	46	from	variation	1614:1622	arg1	measurements					1627:1638	measurements	1627:1638	measurements	1627:1638	The variation in measurements may have an effect on clinical implications, such as therapeutic ranges.
32441664	3	47	theme	measurement	374:384	arg1	performance					350:360	the performance	346:360	the performance of anti-FXa measurement	346:384	In the present study, the performance of anti-FXa measurement was evaluated in order to gain more insight into the clinical applications.
32441664	4	48	theme	variation	507:515	arg1	coefficient					492:502	the longitudinal coefficient	475:502	the longitudinal coefficient of variation (CV)	475:520	Furthermore, the longitudinal coefficient of variation (CV) was studied to investigate whether there is improvement over time.
32441664	1	49	theme	Chromogenic	123:133	arg1	"					204:204	the "gold standard"	186:204	the "gold standard" for monitoring indirect anticoagulants	186:243	Objectives Chromogenic anti-activated factor X (FXa) assays are currently the "gold standard" for monitoring indirect anticoagulants.
32441664	1	49	theme	Chromogenic	123:133	arg1	assays					165:170	Objectives Chromogenic anti-activated factor X (FXa) assays	112:170	Objectives Chromogenic anti-activated factor X (FXa) assays	112:170	Objectives Chromogenic anti-activated factor X (FXa) assays are currently the "gold standard" for monitoring indirect anticoagulants.
32441664	6	50	from	years	817:821	arg1	data					798:801	External quality assessment (EQA) data	764:801	External quality assessment (EQA) data from multiple years	764:821	External quality assessment (EQA) data from multiple years were used from more than 100 laboratories.
32441664	5	51	theme	unfractionated	661:674	arg1	heparin					676:682	unfractionated heparin	661:682	unfractionated heparin (UFH)	661:688	Methods Laboratory tests results were evaluated for samples spiked with unfractionated heparin (UFH), low-molecular-weight-heparin (LMWH), fondaparinux and danaparoid sodium.
32441664	5	51	theme	unfractionated	661:674	arg1	sodium					756:761	fondaparinux and danaparoid sodium	728:761	sodium	756:761	Methods Laboratory tests results were evaluated for samples spiked with unfractionated heparin (UFH), low-molecular-weight-heparin (LMWH), fondaparinux and danaparoid sodium.
32441664	5	51	theme	unfractionated	661:674	arg1	UFH					685:687	UFH	685:687	UFH	685:687	Methods Laboratory tests results were evaluated for samples spiked with unfractionated heparin (UFH), low-molecular-weight-heparin (LMWH), fondaparinux and danaparoid sodium.
32441664	5	51	theme	unfractionated	661:674	arg1	low-molecular-weight-heparin					691:718	low-molecular-weight-heparin	691:718	low-molecular-weight-heparin (LMWH)	691:725	Methods Laboratory tests results were evaluated for samples spiked with unfractionated heparin (UFH), low-molecular-weight-heparin (LMWH), fondaparinux and danaparoid sodium.
32441664	7	52	theme	used	989:992	arg1	methods					994:1000	the frequently used methods	974:1000	the frequently used methods (ANOVA: p < 0.001)	974:1019	Results Comparison of the results for all methods showed significant differences in measured values between the frequently used methods (ANOVA: p < 0.001).
32441664	7	52	theme	used	989:992	arg1	ANOVA					1003:1007	ANOVA	1003:1007	ANOVA: p < 0.001	1003:1018	Results Comparison of the results for all methods showed significant differences in measured values between the frequently used methods (ANOVA: p < 0.001).
32441664	1	53	theme	anti-activated	135:148	arg1	FXa					160:162	FXa	160:162	FXa	160:162	Objectives Chromogenic anti-activated factor X (FXa) assays are currently the "gold standard" for monitoring indirect anticoagulants.
32441664	1	53	theme	anti-activated	135:148	arg1	X					157:157	anti-activated factor X	135:157	Objectives Chromogenic anti-activated factor X (FXa) assays	112:170	Objectives Chromogenic anti-activated factor X (FXa) assays are currently the "gold standard" for monitoring indirect anticoagulants.
32441664	10	54	theme	interlaboratory	1215:1229	arg1	variation					1231:1239	Substantial interlaboratory variation	1203:1239	Substantial interlaboratory variation in anti-FXa monitoring	1203:1262	Substantial interlaboratory variation in anti-FXa monitoring was observed for all parameters, particularly at low concentrations.
32441664	9	55	theme	dextran	1185:1191	arg1	sulphate					1193:1200	dextran sulphate	1185:1200	dextran sulphate	1185:1200	These differences may be caused by differences in method composition, such as the addition of dextran sulphate.
32441664	7	56	theme	measured	950:957	arg1	values					959:964	measured values	950:964	measured values	950:964	Results Comparison of the results for all methods showed significant differences in measured values between the frequently used methods (ANOVA: p < 0.001).
32441664	1	57	theme	factor	150:155	arg1	FXa					160:162	FXa	160:162	FXa	160:162	Objectives Chromogenic anti-activated factor X (FXa) assays are currently the "gold standard" for monitoring indirect anticoagulants.
32441664	1	57	theme	factor	150:155	arg1	X					157:157	anti-activated factor X	135:157	Objectives Chromogenic anti-activated factor X (FXa) assays	112:170	Objectives Chromogenic anti-activated factor X (FXa) assays are currently the "gold standard" for monitoring indirect anticoagulants.
32441664	5	58	dep	Methods	589:595	arg1	results					614:620	results	614:620	Methods Laboratory tests results	589:620	Methods Laboratory tests results were evaluated for samples spiked with unfractionated heparin (UFH), low-molecular-weight-heparin (LMWH), fondaparinux and danaparoid sodium.
32441664	5	58	dep	Methods	589:595	arg1	tests					608:612	Laboratory tests	597:612	Methods Laboratory tests results	589:620	Methods Laboratory tests results were evaluated for samples spiked with unfractionated heparin (UFH), low-molecular-weight-heparin (LMWH), fondaparinux and danaparoid sodium.
32441664	14	59	theme	EQA	1743:1745	arg1	data					1747:1750	the longitudinal EQA data	1726:1750	the longitudinal EQA data	1726:1750	Furthermore, the longitudinal EQA data demonstrated a constant performance and, in at least 50% of the cases, improvement in the CV% of the anti-Xa results over time.
32441664	10	60	theme	low	1313:1315	arg1	concentrations					1317:1330	low concentrations	1313:1330	low concentrations	1313:1330	Substantial interlaboratory variation in anti-FXa monitoring was observed for all parameters, particularly at low concentrations.
32441664	6	61	theme	multiple	808:815	arg1	years					817:821	multiple years	808:821	multiple years	808:821	External quality assessment (EQA) data from multiple years were used from more than 100 laboratories.
32441664	1	62	theme	X	157:157	arg1	"					204:204	the "gold standard"	186:204	the "gold standard" for monitoring indirect anticoagulants	186:243	Objectives Chromogenic anti-activated factor X (FXa) assays are currently the "gold standard" for monitoring indirect anticoagulants.
32441664	1	62	theme	X	157:157	arg1	assays					165:170	Objectives Chromogenic anti-activated factor X (FXa) assays	112:170	Objectives Chromogenic anti-activated factor X (FXa) assays	112:170	Objectives Chromogenic anti-activated factor X (FXa) assays are currently the "gold standard" for monitoring indirect anticoagulants.
32441664	1	63	theme	indirect	221:228	arg1	anticoagulants					230:243	indirect anticoagulants	221:243	indirect anticoagulants	221:243	Objectives Chromogenic anti-activated factor X (FXa) assays are currently the "gold standard" for monitoring indirect anticoagulants.
32441664	5	64	theme	fondaparinux	728:739	arg1	heparin					676:682	unfractionated heparin	661:682	unfractionated heparin (UFH)	661:688	Methods Laboratory tests results were evaluated for samples spiked with unfractionated heparin (UFH), low-molecular-weight-heparin (LMWH), fondaparinux and danaparoid sodium.
32441664	5	64	theme	fondaparinux	728:739	arg1	sodium					756:761	fondaparinux and danaparoid sodium	728:761	sodium	756:761	Methods Laboratory tests results were evaluated for samples spiked with unfractionated heparin (UFH), low-molecular-weight-heparin (LMWH), fondaparinux and danaparoid sodium.
32441664	14	65	theme	CV	1842:1843	arg1	results					1861:1867	the anti-Xa results	1849:1867	the anti-Xa results	1849:1867	Furthermore, the longitudinal EQA data demonstrated a constant performance and, in at least 50% of the cases, improvement in the CV% of the anti-Xa results over time.
32441664	14	65	theme	CV	1842:1843	arg1	%					1844:1844	the CV%	1838:1844	the CV% of the anti-Xa results	1838:1867	Furthermore, the longitudinal EQA data demonstrated a constant performance and, in at least 50% of the cases, improvement in the CV% of the anti-Xa results over time.
32441664	7	66	theme	results	892:898	arg1	Comparison					874:883	Comparison	874:883	Comparison of the results for all methods	874:914	Results Comparison of the results for all methods showed significant differences in measured values between the frequently used methods (ANOVA: p < 0.001).
32441664	11	67	used	used	1461:1464	arg2	results					1426:1432	results	1426:1432	results below this limit	1426:1449	Our results showed that below 0.35 IU/mL, the CVs for UFH and LMWH increase dramatically and results below this limit should be used with caution.
32441664	4	68	theme	longitudinal	479:490	arg1	coefficient					492:502	the longitudinal coefficient	475:502	the longitudinal coefficient of variation (CV)	475:520	Furthermore, the longitudinal coefficient of variation (CV) was studied to investigate whether there is improvement over time.
32441664	14	69	theme	results	1861:1867	arg1	results					1861:1867	the anti-Xa results	1849:1867	the anti-Xa results	1849:1867	Furthermore, the longitudinal EQA data demonstrated a constant performance and, in at least 50% of the cases, improvement in the CV% of the anti-Xa results over time.
32441664	14	69	theme	results	1861:1867	arg1	%					1844:1844	the CV%	1838:1844	the CV% of the anti-Xa results	1838:1867	Furthermore, the longitudinal EQA data demonstrated a constant performance and, in at least 50% of the cases, improvement in the CV% of the anti-Xa results over time.
32441664	7	70	from	differences	935:945	arg1	values					959:964	measured values	950:964	measured values	950:964	Results Comparison of the results for all methods showed significant differences in measured values between the frequently used methods (ANOVA: p < 0.001).
32441664	12	71	theme	method	1547:1552	arg1	important					1570:1578	important	1570:1578	important	1570:1578	Conclusions Our study demonstrates that the choice of the anti-FXa method is particularly important for UFH and LMWH measurement.
32441664	12	71	theme	method	1547:1552	arg1	choice					1524:1529	the choice	1520:1529	the choice of the anti-FXa method	1520:1552	Conclusions Our study demonstrates that the choice of the anti-FXa method is particularly important for UFH and LMWH measurement.
33303871	1	0	theme	dry	219:221	arg1	varieties					223:231	soft, semi-dry, and dry varieties	199:231	soft, semi-dry, and dry varieties	199:231	Date fruits vary widely in the hardness of their edible parts and they are classified accordingly into soft, semi-dry, and dry varieties.
33303871	6	1	theme	soluble	944:950	arg1	sugar					952:956	the soluble sugar and texture parameters	940:979	sugar	952:956	While no correlation was found between the soluble sugar and texture parameters in this study, the different fiber constituents correlated variably with the different parameters of date fruit texture.
33303871	1	2	theme	soft	199:202	arg1	varieties					223:231	soft, semi-dry, and dry varieties	199:231	soft, semi-dry, and dry varieties	199:231	Date fruits vary widely in the hardness of their edible parts and they are classified accordingly into soft, semi-dry, and dry varieties.
33303871	2	3	theme	sucrose	403:409	arg1	ratio					394:398	the ratio	390:398	the ratio of sucrose to reducing sugars	390:428	Fruit texture, a significant parameter in determining consumer acceptance, is related to the tissue structure and chemical composition of the fruit, mainly the ratio of sucrose to reducing sugars.
33303871	2	4	theme	consumer	288:295	arg1	acceptance					297:306	consumer acceptance	288:306	consumer acceptance	288:306	Fruit texture, a significant parameter in determining consumer acceptance, is related to the tissue structure and chemical composition of the fruit, mainly the ratio of sucrose to reducing sugars.
33303871	6	5	theme	fruit	1087:1091	arg1	texture					1093:1099	date fruit texture	1082:1099	date fruit texture	1082:1099	While no correlation was found between the soluble sugar and texture parameters in this study, the different fiber constituents correlated variably with the different parameters of date fruit texture.
33303871	4	6	theme	fruits	655:660	arg1	80 g/100 g					637:646	ca 80 g/100 g	634:646	ca 80 g/100 g of the fruits	634:660	The soluble sugars, glucose and fructose, represent ca 80 g/100 g of the fruits on the basis of dry weight (DW) while the dietary fiber contents varied 5.2-7.4 g/100 dg D.W. with lignin being the main determinant of the variability.
33303871	1	7	theme	semi-dry	205:212	arg1	varieties					223:231	soft, semi-dry, and dry varieties	199:231	soft, semi-dry, and dry varieties	199:231	Date fruits vary widely in the hardness of their edible parts and they are classified accordingly into soft, semi-dry, and dry varieties.
33303871	6	8	theme	different	1058:1066	arg1	parameters					1068:1077	the different parameters	1054:1077	the different parameters of date fruit texture	1054:1099	While no correlation was found between the soluble sugar and texture parameters in this study, the different fiber constituents correlated variably with the different parameters of date fruit texture.
33303871	6	9	theme	date	1082:1085	arg1	texture					1093:1099	date fruit texture	1082:1099	date fruit texture	1082:1099	While no correlation was found between the soluble sugar and texture parameters in this study, the different fiber constituents correlated variably with the different parameters of date fruit texture.
33303871	2	10	theme	significant	251:261	arg1	texture					240:246	Fruit texture	234:246	Fruit texture	234:246	Fruit texture, a significant parameter in determining consumer acceptance, is related to the tissue structure and chemical composition of the fruit, mainly the ratio of sucrose to reducing sugars.
33303871	2	10	theme	significant	251:261	arg1	parameter					263:271	a significant parameter	249:271	a significant parameter in determining consumer acceptance	249:306	Fruit texture, a significant parameter in determining consumer acceptance, is related to the tissue structure and chemical composition of the fruit, mainly the ratio of sucrose to reducing sugars.
33303871	2	11	theme	chemical	348:355	arg1	composition					357:367	chemical composition	348:367	chemical composition	348:367	Fruit texture, a significant parameter in determining consumer acceptance, is related to the tissue structure and chemical composition of the fruit, mainly the ratio of sucrose to reducing sugars.
33303871	4	12	theme	dry	678:680	arg1	DW					690:691	DW	690:691	DW	690:691	The soluble sugars, glucose and fructose, represent ca 80 g/100 g of the fruits on the basis of dry weight (DW) while the dietary fiber contents varied 5.2-7.4 g/100 dg D.W. with lignin being the main determinant of the variability.
33303871	4	12	theme	dry	678:680	arg1	weight					682:687	dry weight	678:687	dry weight (DW)	678:692	The soluble sugars, glucose and fructose, represent ca 80 g/100 g of the fruits on the basis of dry weight (DW) while the dietary fiber contents varied 5.2-7.4 g/100 dg D.W. with lignin being the main determinant of the variability.
33303871	7	13	theme	related	1216:1222	arg1	parameters					1224:1233	the related parameters	1212:1233	the related parameters	1212:1233	Lignin, arabinoxylan, galactomannan, and pectin were found to correlate significantly with fruit hardness and the related parameters, gumminess and chewiness.
33303871	7	13	theme	related	1216:1222	arg1	chewiness					1250:1258	chewiness	1250:1258	chewiness	1250:1258	Lignin, arabinoxylan, galactomannan, and pectin were found to correlate significantly with fruit hardness and the related parameters, gumminess and chewiness.
33303871	7	13	theme	related	1216:1222	arg1	gumminess					1236:1244	gumminess	1236:1244	gumminess	1236:1244	Lignin, arabinoxylan, galactomannan, and pectin were found to correlate significantly with fruit hardness and the related parameters, gumminess and chewiness.
33303871	2	14	theme	tissue	327:332	arg1	structure					334:342	tissue structure	327:342	tissue structure	327:342	Fruit texture, a significant parameter in determining consumer acceptance, is related to the tissue structure and chemical composition of the fruit, mainly the ratio of sucrose to reducing sugars.
33303871	0	15	theme	Dietary	0:6	arg1	fiber					8:12	Dietary fiber	0:12	Dietary fiber	0:12	Dietary fiber components, microstructure, and texture of date fruits (Phoenix dactylifera, L.).
33303871	0	16	theme	fruits	62:67	arg1	fiber					8:12	Dietary fiber	0:12	Dietary fiber	0:12	Dietary fiber components, microstructure, and texture of date fruits (Phoenix dactylifera, L.).
33303871	0	16	theme	fruits	62:67	arg1	microstructure					26:39	microstructure	26:39	microstructure	26:39	Dietary fiber components, microstructure, and texture of date fruits (Phoenix dactylifera, L.).
33303871	0	16	theme	fruits	62:67	arg1	texture					46:52	texture	46:52	texture of date fruits	46:67	Dietary fiber components, microstructure, and texture of date fruits (Phoenix dactylifera, L.).
33303871	3	17	theme	chemical	491:498	arg1	composition					500:510	the chemical composition	487:510	the chemical composition	487:510	This study aimed to understand the relationship between the chemical composition, microstructure, and texture profile of 10 major Emirati date fruits.
33303871	8	18	theme	strong	1347:1352	arg1	correlation					1354:1364	a strong correlation	1345:1364	a strong correlation with cohesiveness	1345:1382	Both lignin and arabinoxylan correlated with resilience, and arabinoxylan exhibited a strong correlation with cohesiveness.
33303871	6	19	theme	texture	962:968	arg1	parameters					970:979	the soluble sugar and texture parameters	940:979	parameters	970:979	While no correlation was found between the soluble sugar and texture parameters in this study, the different fiber constituents correlated variably with the different parameters of date fruit texture.
33303871	2	20	theme	fruit	376:380	arg1	structure					334:342	tissue structure	327:342	tissue structure	327:342	Fruit texture, a significant parameter in determining consumer acceptance, is related to the tissue structure and chemical composition of the fruit, mainly the ratio of sucrose to reducing sugars.
33303871	2	20	theme	fruit	376:380	arg1	composition					357:367	chemical composition	348:367	chemical composition	348:367	Fruit texture, a significant parameter in determining consumer acceptance, is related to the tissue structure and chemical composition of the fruit, mainly the ratio of sucrose to reducing sugars.
33303871	4	21	theme	ca	634:635	arg1	80 g/100 g					637:646	ca 80 g/100 g	634:646	ca 80 g/100 g of the fruits	634:660	The soluble sugars, glucose and fructose, represent ca 80 g/100 g of the fruits on the basis of dry weight (DW) while the dietary fiber contents varied 5.2-7.4 g/100 dg D.W. with lignin being the main determinant of the variability.
33303871	6	22	from	sugar	952:956	arg1	study					989:993	this study	984:993	this study	984:993	While no correlation was found between the soluble sugar and texture parameters in this study, the different fiber constituents correlated variably with the different parameters of date fruit texture.
33303871	4	23	theme	soluble	586:592	arg1	fructose					614:621	fructose	614:621	fructose	614:621	The soluble sugars, glucose and fructose, represent ca 80 g/100 g of the fruits on the basis of dry weight (DW) while the dietary fiber contents varied 5.2-7.4 g/100 dg D.W. with lignin being the main determinant of the variability.
33303871	4	23	theme	soluble	586:592	arg1	glucose					602:608	glucose	602:608	glucose	602:608	The soluble sugars, glucose and fructose, represent ca 80 g/100 g of the fruits on the basis of dry weight (DW) while the dietary fiber contents varied 5.2-7.4 g/100 dg D.W. with lignin being the main determinant of the variability.
33303871	4	23	theme	soluble	586:592	arg1	sugars					594:599	The soluble sugars	582:599	The soluble sugars	582:599	The soluble sugars, glucose and fructose, represent ca 80 g/100 g of the fruits on the basis of dry weight (DW) while the dietary fiber contents varied 5.2-7.4 g/100 dg D.W. with lignin being the main determinant of the variability.
33303871	0	24	dep	fiber	8:12	arg1	components					14:23	components	14:23	components	14:23	Dietary fiber components, microstructure, and texture of date fruits (Phoenix dactylifera, L.).
33303871	0	24	dep	fiber	8:12	arg1	dactylifera					78:88	dactylifera	78:88	dactylifera	78:88	Dietary fiber components, microstructure, and texture of date fruits (Phoenix dactylifera, L.).
33303871	0	24	dep	fiber	8:12	arg1	L					91:91	L	91:91	L	91:91	Dietary fiber components, microstructure, and texture of date fruits (Phoenix dactylifera, L.).
33303871	6	25	from	parameters	970:979	arg1	study					989:993	this study	984:993	this study	984:993	While no correlation was found between the soluble sugar and texture parameters in this study, the different fiber constituents correlated variably with the different parameters of date fruit texture.
33303871	5	26	theme	samples	835:841	arg1	textures					819:826	The textures	815:826	The textures of the samples	815:841	The textures of the samples were studied using instrumental texture profile analysis.
33303871	1	27	theme	edible	145:150	arg1	parts					152:156	their edible parts	139:156	their edible parts	139:156	Date fruits vary widely in the hardness of their edible parts and they are classified accordingly into soft, semi-dry, and dry varieties.
33303871	6	28	theme	different	1000:1008	arg1	constituents					1016:1027	the different fiber constituents	996:1027	the different fiber constituents	996:1027	While no correlation was found between the soluble sugar and texture parameters in this study, the different fiber constituents correlated variably with the different parameters of date fruit texture.
33303871	4	29	theme	variability	802:812	arg1	lignin					761:766	lignin	761:766	lignin	761:766	The soluble sugars, glucose and fructose, represent ca 80 g/100 g of the fruits on the basis of dry weight (DW) while the dietary fiber contents varied 5.2-7.4 g/100 dg D.W. with lignin being the main determinant of the variability.
33303871	4	29	theme	variability	802:812	arg1	determinant					783:793	the main determinant	774:793	the main determinant of the variability	774:812	The soluble sugars, glucose and fructose, represent ca 80 g/100 g of the fruits on the basis of dry weight (DW) while the dietary fiber contents varied 5.2-7.4 g/100 dg D.W. with lignin being the main determinant of the variability.
33303871	3	30	theme	date	569:572	arg1	fruits					574:579	10 major Emirati date fruits	552:579	10 major Emirati date fruits	552:579	This study aimed to understand the relationship between the chemical composition, microstructure, and texture profile of 10 major Emirati date fruits.
33303871	2	31	theme	Fruit	234:238	arg1	texture					240:246	Fruit texture	234:246	Fruit texture	234:246	Fruit texture, a significant parameter in determining consumer acceptance, is related to the tissue structure and chemical composition of the fruit, mainly the ratio of sucrose to reducing sugars.
33303871	2	31	theme	Fruit	234:238	arg1	parameter					263:271	a significant parameter	249:271	a significant parameter in determining consumer acceptance	249:306	Fruit texture, a significant parameter in determining consumer acceptance, is related to the tissue structure and chemical composition of the fruit, mainly the ratio of sucrose to reducing sugars.
33303871	1	32	theme	parts	152:156	arg1	hardness					127:134	the hardness	123:134	the hardness of their edible parts	123:156	Date fruits vary widely in the hardness of their edible parts and they are classified accordingly into soft, semi-dry, and dry varieties.
33303871	8	33	with	correlation	1354:1364	arg1	cohesiveness					1371:1382	cohesiveness	1371:1382	cohesiveness	1371:1382	Both lignin and arabinoxylan correlated with resilience, and arabinoxylan exhibited a strong correlation with cohesiveness.
33303871	3	34	theme	fruits	574:579	arg1	profile					541:547	texture profile	533:547	texture profile	533:547	This study aimed to understand the relationship between the chemical composition, microstructure, and texture profile of 10 major Emirati date fruits.
33303871	3	34	theme	fruits	574:579	arg1	microstructure					513:526	microstructure	513:526	microstructure	513:526	This study aimed to understand the relationship between the chemical composition, microstructure, and texture profile of 10 major Emirati date fruits.
33303871	3	34	theme	fruits	574:579	arg1	composition					500:510	the chemical composition	487:510	the chemical composition	487:510	This study aimed to understand the relationship between the chemical composition, microstructure, and texture profile of 10 major Emirati date fruits.
33303871	4	35	dep	weight	682:687	arg1	basis					669:673	basis	669:673	basis	669:673	The soluble sugars, glucose and fructose, represent ca 80 g/100 g of the fruits on the basis of dry weight (DW) while the dietary fiber contents varied 5.2-7.4 g/100 dg D.W. with lignin being the main determinant of the variability.
33303871	4	35	dep	weight	682:687	arg1	the					665:667	the	665:667	the	665:667	The soluble sugars, glucose and fructose, represent ca 80 g/100 g of the fruits on the basis of dry weight (DW) while the dietary fiber contents varied 5.2-7.4 g/100 dg D.W. with lignin being the main determinant of the variability.
33303871	7	36	theme	fruit	1193:1197	arg1	hardness					1199:1206	fruit hardness	1193:1206	fruit hardness	1193:1206	Lignin, arabinoxylan, galactomannan, and pectin were found to correlate significantly with fruit hardness and the related parameters, gumminess and chewiness.
33303871	3	37	theme	major	555:559	arg1	date					569:572	major Emirati date	555:572	10 major Emirati date fruits	552:579	This study aimed to understand the relationship between the chemical composition, microstructure, and texture profile of 10 major Emirati date fruits.
33303871	2	38	dep	structure	334:342	arg1	the					323:325	the	323:325	the	323:325	Fruit texture, a significant parameter in determining consumer acceptance, is related to the tissue structure and chemical composition of the fruit, mainly the ratio of sucrose to reducing sugars.
33303871	3	39	theme	Emirati	561:567	arg1	date					569:572	major Emirati date	555:572	10 major Emirati date fruits	552:579	This study aimed to understand the relationship between the chemical composition, microstructure, and texture profile of 10 major Emirati date fruits.
33303871	4	40	theme	fiber	712:716	arg1	contents					718:725	the dietary fiber contents	700:725	the dietary fiber contents	700:725	The soluble sugars, glucose and fructose, represent ca 80 g/100 g of the fruits on the basis of dry weight (DW) while the dietary fiber contents varied 5.2-7.4 g/100 dg D.W. with lignin being the main determinant of the variability.
33303871	4	41	theme	main	778:781	arg1	lignin					761:766	lignin	761:766	lignin	761:766	The soluble sugars, glucose and fructose, represent ca 80 g/100 g of the fruits on the basis of dry weight (DW) while the dietary fiber contents varied 5.2-7.4 g/100 dg D.W. with lignin being the main determinant of the variability.
33303871	4	41	theme	main	778:781	arg1	determinant					783:793	the main determinant	774:793	the main determinant of the variability	774:812	The soluble sugars, glucose and fructose, represent ca 80 g/100 g of the fruits on the basis of dry weight (DW) while the dietary fiber contents varied 5.2-7.4 g/100 dg D.W. with lignin being the main determinant of the variability.
33303871	4	42	theme	dietary	704:710	arg1	contents					718:725	the dietary fiber contents	700:725	the dietary fiber contents	700:725	The soluble sugars, glucose and fructose, represent ca 80 g/100 g of the fruits on the basis of dry weight (DW) while the dietary fiber contents varied 5.2-7.4 g/100 dg D.W. with lignin being the main determinant of the variability.
33303871	5	43	theme	profile	883:889	arg1	analysis					891:898	instrumental texture profile analysis	862:898	instrumental texture profile analysis	862:898	The textures of the samples were studied using instrumental texture profile analysis.
33303871	6	44	theme	fiber	1010:1014	arg1	constituents					1016:1027	the different fiber constituents	996:1027	the different fiber constituents	996:1027	While no correlation was found between the soluble sugar and texture parameters in this study, the different fiber constituents correlated variably with the different parameters of date fruit texture.
33303871	5	45	theme	texture	875:881	arg1	analysis					891:898	instrumental texture profile analysis	862:898	instrumental texture profile analysis	862:898	The textures of the samples were studied using instrumental texture profile analysis.
33303871	1	46	theme	Date	96:99	arg1	fruits					101:106	Date fruits	96:106	Date fruits	96:106	Date fruits vary widely in the hardness of their edible parts and they are classified accordingly into soft, semi-dry, and dry varieties.
33303871	0	47	theme	date	57:60	arg1	fruits					62:67	date fruits	57:67	date fruits	57:67	Dietary fiber components, microstructure, and texture of date fruits (Phoenix dactylifera, L.).
33303871	5	48	theme	instrumental	862:873	arg1	analysis					891:898	instrumental texture profile analysis	862:898	instrumental texture profile analysis	862:898	The textures of the samples were studied using instrumental texture profile analysis.
33303871	3	49	theme	texture	533:539	arg1	profile					541:547	texture profile	533:547	texture profile	533:547	This study aimed to understand the relationship between the chemical composition, microstructure, and texture profile of 10 major Emirati date fruits.
33303871	2	50	theme	reducing	414:421	arg1	sugars					423:428	reducing sugars	414:428	reducing sugars	414:428	Fruit texture, a significant parameter in determining consumer acceptance, is related to the tissue structure and chemical composition of the fruit, mainly the ratio of sucrose to reducing sugars.
33303871	6	51	theme	texture	1093:1099	arg1	parameters					1068:1077	the different parameters	1054:1077	the different parameters of date fruit texture	1054:1099	While no correlation was found between the soluble sugar and texture parameters in this study, the different fiber constituents correlated variably with the different parameters of date fruit texture.
33032254	0	0	theme	trapped	92:98	arg1	spectrometry					113:124	trapped ion mobility spectrometry	92:124	trapped ion mobility spectrometry	92:124	Discrimination of isomeric trisaccharides and their relative quantification in honeys using trapped ion mobility spectrometry.
33032254	7	1	theme	suitable	1171:1178	arg1	analysis					1188:1195	suitable routine analysis	1171:1195	suitable routine analysis	1171:1195	Moreover, such direct approach constitutes an alternative tool to current longer chromatographic runs, paving the way to a transfer as suitable routine analysis.
33032254	1	2	theme	roles	167:171	arg1	myriad					148:153	a myriad	146:153	a myriad of critical roles	146:171	Carbohydrates play a myriad of critical roles as key intermediaries for energy storage, cell wall constituents, or also fuel for organisms.
33032254	1	2	theme	roles	167:171	arg1	intermediaries					180:193	key intermediaries	176:193	key intermediaries for energy storage, cell wall constituents, or also fuel for organisms	176:264	Carbohydrates play a myriad of critical roles as key intermediaries for energy storage, cell wall constituents, or also fuel for organisms.
33032254	7	3	theme	routine	1180:1186	arg1	analysis					1188:1195	suitable routine analysis	1171:1195	suitable routine analysis	1171:1195	Moreover, such direct approach constitutes an alternative tool to current longer chromatographic runs, paving the way to a transfer as suitable routine analysis.
33032254	7	4	theme	such	1046:1049	arg1	approach					1058:1065	such direct approach	1046:1065	such direct approach	1046:1065	Moreover, such direct approach constitutes an alternative tool to current longer chromatographic runs, paving the way to a transfer as suitable routine analysis.
33032254	0	5	theme	mobility	104:111	arg1	spectrometry					113:124	trapped ion mobility spectrometry	92:124	trapped ion mobility spectrometry	92:124	Discrimination of isomeric trisaccharides and their relative quantification in honeys using trapped ion mobility spectrometry.
33032254	0	6	from	Discrimination	0:13	arg1	honeys					79:84	honeys	79:84	honeys using trapped ion mobility spectrometry	79:124	Discrimination of isomeric trisaccharides and their relative quantification in honeys using trapped ion mobility spectrometry.
33032254	2	7	theme	multiple	286:293	arg1	isomers					306:312	multiple structural isomers	286:312	multiple structural isomers based on the monosaccharides composition (stereoisomers), the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β),	286:459	The deciphering of multiple structural isomers based on the monosaccharides composition (stereoisomers), the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β), remains a major analytical challenging task.
33032254	3	8	theme	underivatized	541:553	arg1	trisaccharides					564:577	13 underivatized isomeric trisaccharides	538:577	13 underivatized isomeric trisaccharides	538:577	The possibility to discriminate 13 underivatized isomeric trisaccharides were reported using electrospray ionization coupled to trapped ion mobility spectrometry (ESI-TIMS).
33032254	0	9	theme	ion	100:102	arg1	spectrometry					113:124	trapped ion mobility spectrometry	92:124	trapped ion mobility spectrometry	92:124	Discrimination of isomeric trisaccharides and their relative quantification in honeys using trapped ion mobility spectrometry.
33032254	0	10	from	quantification	61:74	arg1	honeys					79:84	honeys	79:84	honeys using trapped ion mobility spectrometry	79:124	Discrimination of isomeric trisaccharides and their relative quantification in honeys using trapped ion mobility spectrometry.
33032254	7	11	theme	alternative	1082:1092	arg1	tool					1094:1097	an alternative tool	1079:1097	an alternative tool to current longer chromatographic runs	1079:1136	Moreover, such direct approach constitutes an alternative tool to current longer chromatographic runs, paving the way to a transfer as suitable routine analysis.
33032254	4	12	theme	ratio	707:711	arg1	optimization					686:697	optimization	686:697	optimization of scan ratio enhancing both the mobility resolving power (R) and resolution (r)	686:778	After optimization of scan ratio enhancing both the mobility resolving power (R) and resolution (r), fingerprints from 5 different honeys were obtained.
33032254	2	13	theme	challenging	488:498	arg1	task					500:503	a major analytical challenging task	469:503	a major analytical challenging task	469:503	The deciphering of multiple structural isomers based on the monosaccharides composition (stereoisomers), the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β), remains a major analytical challenging task.
33032254	7	14	dep	current	1102:1108	arg1	chromatographic					1117:1131	chromatographic	1117:1131	chromatographic	1117:1131	Moreover, such direct approach constitutes an alternative tool to current longer chromatographic runs, paving the way to a transfer as suitable routine analysis.
33032254	5	15	dep	58.3	896:899	arg1	to					893:894	to	893:894	to	893:894	Seven trisaccharides with relative content varying from 1.5 to 58.3%, were identified.
33032254	1	16	theme	key	176:178	arg1	myriad					148:153	a myriad	146:153	a myriad of critical roles	146:171	Carbohydrates play a myriad of critical roles as key intermediaries for energy storage, cell wall constituents, or also fuel for organisms.
33032254	1	16	theme	key	176:178	arg1	intermediaries					180:193	key intermediaries	176:193	key intermediaries for energy storage, cell wall constituents, or also fuel for organisms	176:264	Carbohydrates play a myriad of critical roles as key intermediaries for energy storage, cell wall constituents, or also fuel for organisms.
33032254	2	17	theme	analytical	477:486	arg1	task					500:503	a major analytical challenging task	469:503	a major analytical challenging task	469:503	The deciphering of multiple structural isomers based on the monosaccharides composition (stereoisomers), the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β), remains a major analytical challenging task.
33032254	2	18	theme	glycosidic	384:393	arg1	connectivity					405:416	connectivity	405:416	connectivity	405:416	The deciphering of multiple structural isomers based on the monosaccharides composition (stereoisomers), the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β), remains a major analytical challenging task.
33032254	2	18	theme	glycosidic	384:393	arg1	linkages					395:402	glycosidic linkages	384:402	glycosidic linkages (connectivity)	384:417	The deciphering of multiple structural isomers based on the monosaccharides composition (stereoisomers), the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β), remains a major analytical challenging task.
33032254	6	19	used	used	996:999	arg2	content					960:966	their relative content	945:966	their relative content	945:966	It was demonstrated that their relative content and/or their ratio could be used to ascertain origin of the honeys.
33032254	6	19	used	used	996:999	arg2	ratio					981:985	their ratio	975:985	their ratio	975:985	It was demonstrated that their relative content and/or their ratio could be used to ascertain origin of the honeys.
33032254	3	20	theme	isomeric	555:562	arg1	trisaccharides					564:577	13 underivatized isomeric trisaccharides	538:577	13 underivatized isomeric trisaccharides	538:577	The possibility to discriminate 13 underivatized isomeric trisaccharides were reported using electrospray ionization coupled to trapped ion mobility spectrometry (ESI-TIMS).
33032254	5	21	theme	relative	859:866	arg1	content					868:874	relative content	859:874	relative content	859:874	Seven trisaccharides with relative content varying from 1.5 to 58.3%, were identified.
33032254	2	22	theme	configuration	436:448	arg1	composition					343:353	the monosaccharides composition	323:353	the monosaccharides composition (stereoisomers)	323:369	The deciphering of multiple structural isomers based on the monosaccharides composition (stereoisomers), the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β), remains a major analytical challenging task.
33032254	2	22	theme	configuration	436:448	arg1	type					376:379	the type	372:379	the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β)	372:458	The deciphering of multiple structural isomers based on the monosaccharides composition (stereoisomers), the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β), remains a major analytical challenging task.
33032254	6	23	theme	relative	951:958	arg1	content					960:966	their relative content	945:966	their relative content	945:966	It was demonstrated that their relative content and/or their ratio could be used to ascertain origin of the honeys.
33032254	2	24	dep	configuration	436:448	arg1	β					457:457	β	457:457	β	457:457	The deciphering of multiple structural isomers based on the monosaccharides composition (stereoisomers), the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β), remains a major analytical challenging task.
33032254	2	24	dep	configuration	436:448	arg1	α					451:451	α	451:451	α	451:451	The deciphering of multiple structural isomers based on the monosaccharides composition (stereoisomers), the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β), remains a major analytical challenging task.
33032254	4	25	theme	resolving	741:749	arg1	R					758:758	R	758:758	R	758:758	After optimization of scan ratio enhancing both the mobility resolving power (R) and resolution (r), fingerprints from 5 different honeys were obtained.
33032254	4	25	theme	resolving	741:749	arg1	power					751:755	the mobility resolving power	728:755	the mobility resolving power (R)	728:759	After optimization of scan ratio enhancing both the mobility resolving power (R) and resolution (r), fingerprints from 5 different honeys were obtained.
33032254	1	26	theme	energy	199:204	arg1	storage					206:212	energy storage	199:212	energy storage	199:212	Carbohydrates play a myriad of critical roles as key intermediaries for energy storage, cell wall constituents, or also fuel for organisms.
33032254	0	27	theme	trisaccharides	27:40	arg1	Discrimination					0:13	Discrimination	0:13	Discrimination of isomeric trisaccharides	0:40	Discrimination of isomeric trisaccharides and their relative quantification in honeys using trapped ion mobility spectrometry.
33032254	0	27	theme	trisaccharides	27:40	arg1	quantification					61:74	their relative quantification	46:74	their relative quantification in honeys using trapped ion mobility spectrometry	46:124	Discrimination of isomeric trisaccharides and their relative quantification in honeys using trapped ion mobility spectrometry.
33032254	0	28	theme	isomeric	18:25	arg1	trisaccharides					27:40	isomeric trisaccharides	18:40	isomeric trisaccharides	18:40	Discrimination of isomeric trisaccharides and their relative quantification in honeys using trapped ion mobility spectrometry.
33032254	2	29	theme	structural	295:304	arg1	isomers					306:312	multiple structural isomers	286:312	multiple structural isomers based on the monosaccharides composition (stereoisomers), the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β),	286:459	The deciphering of multiple structural isomers based on the monosaccharides composition (stereoisomers), the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β), remains a major analytical challenging task.
33032254	7	30	theme	direct	1051:1056	arg1	approach					1058:1065	such direct approach	1046:1065	such direct approach	1046:1065	Moreover, such direct approach constitutes an alternative tool to current longer chromatographic runs, paving the way to a transfer as suitable routine analysis.
33032254	6	31	theme	honeys	1028:1033	arg1	origin					1014:1019	origin	1014:1019	origin of the honeys	1014:1033	It was demonstrated that their relative content and/or their ratio could be used to ascertain origin of the honeys.
33032254	1	32	theme	cell	215:218	arg1	constituents					225:236	cell wall constituents	215:236	cell wall constituents	215:236	Carbohydrates play a myriad of critical roles as key intermediaries for energy storage, cell wall constituents, or also fuel for organisms.
33032254	2	33	theme	linkages	395:402	arg1	composition					343:353	the monosaccharides composition	323:353	the monosaccharides composition (stereoisomers)	323:369	The deciphering of multiple structural isomers based on the monosaccharides composition (stereoisomers), the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β), remains a major analytical challenging task.
33032254	2	33	theme	linkages	395:402	arg1	type					376:379	the type	372:379	the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β)	372:458	The deciphering of multiple structural isomers based on the monosaccharides composition (stereoisomers), the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β), remains a major analytical challenging task.
33032254	3	34	theme	ion	642:644	arg1	spectrometry					655:666	trapped ion mobility spectrometry	634:666	trapped ion mobility spectrometry (ESI-TIMS)	634:677	The possibility to discriminate 13 underivatized isomeric trisaccharides were reported using electrospray ionization coupled to trapped ion mobility spectrometry (ESI-TIMS).
33032254	3	34	theme	ion	642:644	arg1	ESI-TIMS					669:676	ESI-TIMS	669:676	ESI-TIMS	669:676	The possibility to discriminate 13 underivatized isomeric trisaccharides were reported using electrospray ionization coupled to trapped ion mobility spectrometry (ESI-TIMS).
33032254	2	35	theme	anomeric	427:434	arg1	configuration					436:448	the anomeric configuration	423:448	the anomeric configuration (α and β)	423:458	The deciphering of multiple structural isomers based on the monosaccharides composition (stereoisomers), the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β), remains a major analytical challenging task.
33032254	4	36	from	honeys	811:816	arg1	fingerprints					781:792	fingerprints	781:792	fingerprints from 5 different honeys	781:816	After optimization of scan ratio enhancing both the mobility resolving power (R) and resolution (r), fingerprints from 5 different honeys were obtained.
33032254	1	37	theme	wall	220:223	arg1	constituents					225:236	cell wall constituents	215:236	cell wall constituents	215:236	Carbohydrates play a myriad of critical roles as key intermediaries for energy storage, cell wall constituents, or also fuel for organisms.
33032254	7	38	theme	current	1102:1108	arg1	runs					1133:1136	current longer chromatographic runs	1102:1136	current longer chromatographic runs	1102:1136	Moreover, such direct approach constitutes an alternative tool to current longer chromatographic runs, paving the way to a transfer as suitable routine analysis.
33032254	3	39	theme	mobility	646:653	arg1	spectrometry					655:666	trapped ion mobility spectrometry	634:666	trapped ion mobility spectrometry (ESI-TIMS)	634:677	The possibility to discriminate 13 underivatized isomeric trisaccharides were reported using electrospray ionization coupled to trapped ion mobility spectrometry (ESI-TIMS).
33032254	3	39	theme	mobility	646:653	arg1	ESI-TIMS					669:676	ESI-TIMS	669:676	ESI-TIMS	669:676	The possibility to discriminate 13 underivatized isomeric trisaccharides were reported using electrospray ionization coupled to trapped ion mobility spectrometry (ESI-TIMS).
33032254	0	40	theme	relative	52:59	arg1	quantification					61:74	their relative quantification	46:74	their relative quantification in honeys using trapped ion mobility spectrometry	46:124	Discrimination of isomeric trisaccharides and their relative quantification in honeys using trapped ion mobility spectrometry.
33032254	7	41	theme	a	1157:1157	arg1	transfer					1159:1166	a transfer	1157:1166	a transfer	1157:1166	Moreover, such direct approach constitutes an alternative tool to current longer chromatographic runs, paving the way to a transfer as suitable routine analysis.
33032254	4	42	theme	different	801:809	arg1	honeys					811:816	5 different honeys	799:816	5 different honeys	799:816	After optimization of scan ratio enhancing both the mobility resolving power (R) and resolution (r), fingerprints from 5 different honeys were obtained.
33032254	5	43	with	trisaccharides	839:852	arg1	content					868:874	relative content	859:874	relative content	859:874	Seven trisaccharides with relative content varying from 1.5 to 58.3%, were identified.
33032254	3	44	theme	electrospray	599:610	arg1	ionization					612:621	electrospray ionization	599:621	electrospray ionization coupled to trapped ion mobility spectrometry (ESI-TIMS)	599:677	The possibility to discriminate 13 underivatized isomeric trisaccharides were reported using electrospray ionization coupled to trapped ion mobility spectrometry (ESI-TIMS).
33032254	2	45	theme	monosaccharides	327:341	arg1	composition					343:353	the monosaccharides composition	323:353	the monosaccharides composition (stereoisomers)	323:369	The deciphering of multiple structural isomers based on the monosaccharides composition (stereoisomers), the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β), remains a major analytical challenging task.
33032254	2	45	theme	monosaccharides	327:341	arg1	type					376:379	the type	372:379	the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β)	372:458	The deciphering of multiple structural isomers based on the monosaccharides composition (stereoisomers), the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β), remains a major analytical challenging task.
33032254	2	45	theme	monosaccharides	327:341	arg1	stereoisomers					356:368	stereoisomers	356:368	stereoisomers	356:368	The deciphering of multiple structural isomers based on the monosaccharides composition (stereoisomers), the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β), remains a major analytical challenging task.
33032254	3	46	theme	trapped	634:640	arg1	spectrometry					655:666	trapped ion mobility spectrometry	634:666	trapped ion mobility spectrometry (ESI-TIMS)	634:677	The possibility to discriminate 13 underivatized isomeric trisaccharides were reported using electrospray ionization coupled to trapped ion mobility spectrometry (ESI-TIMS).
33032254	3	46	theme	trapped	634:640	arg1	ESI-TIMS					669:676	ESI-TIMS	669:676	ESI-TIMS	669:676	The possibility to discriminate 13 underivatized isomeric trisaccharides were reported using electrospray ionization coupled to trapped ion mobility spectrometry (ESI-TIMS).
33032254	4	47	theme	mobility	732:739	arg1	R					758:758	R	758:758	R	758:758	After optimization of scan ratio enhancing both the mobility resolving power (R) and resolution (r), fingerprints from 5 different honeys were obtained.
33032254	4	47	theme	mobility	732:739	arg1	power					751:755	the mobility resolving power	728:755	the mobility resolving power (R)	728:759	After optimization of scan ratio enhancing both the mobility resolving power (R) and resolution (r), fingerprints from 5 different honeys were obtained.
33032254	2	48	theme	major	471:475	arg1	task					500:503	a major analytical challenging task	469:503	a major analytical challenging task	469:503	The deciphering of multiple structural isomers based on the monosaccharides composition (stereoisomers), the type of glycosidic linkages (connectivity) and the anomeric configuration (α and β), remains a major analytical challenging task.
33032254	1	49	theme	critical	158:165	arg1	roles					167:171	critical roles	158:171	critical roles	158:171	Carbohydrates play a myriad of critical roles as key intermediaries for energy storage, cell wall constituents, or also fuel for organisms.
33032254	4	50	dep	ratio	707:711	arg1	scan					702:705	scan	702:705	scan	702:705	After optimization of scan ratio enhancing both the mobility resolving power (R) and resolution (r), fingerprints from 5 different honeys were obtained.
34323370	6	0	from	256	1121:1123	arg1	bands					1112:1116	two absorbance bands	1097:1116	two absorbance bands at 256 and 358 nm	1097:1134	The spray-coated paper documents exhibit two absorbance bands at 256 and 358 nm, and two fluorescence peaks at 433 and 511 nm.
34323370	6	0	from	256	1121:1123	arg1	peaks					1158:1162	two fluorescence peaks	1141:1162	two fluorescence peaks at 433 and 511 nm	1141:1180	The spray-coated paper documents exhibit two absorbance bands at 256 and 358 nm, and two fluorescence peaks at 433 and 511 nm.
34323370	2	1	theme	oxide	457:461	arg1	pigment					463:469	the phosphorescent strontium aluminum oxide pigment	419:469	the phosphorescent strontium aluminum oxide pigment	419:469	To generate a translucent film from pigment/polyacrylic nanocomposite, the phosphorescent strontium aluminum oxide pigment should be well-dispersed in the solution of the polyacrylic-based binder without agglomeration.
34323370	2	1	theme	oxide	457:461	arg1	well-dispersed					481:494	well-dispersed	481:494	well-dispersed	481:494	To generate a translucent film from pigment/polyacrylic nanocomposite, the phosphorescent strontium aluminum oxide pigment should be well-dispersed in the solution of the polyacrylic-based binder without agglomeration.
34323370	6	2	from	358 nm	1129:1134	arg1	bands					1112:1116	two absorbance bands	1097:1116	two absorbance bands at 256 and 358 nm	1097:1134	The spray-coated paper documents exhibit two absorbance bands at 256 and 358 nm, and two fluorescence peaks at 433 and 511 nm.
34323370	6	2	from	358 nm	1129:1134	arg1	peaks					1158:1162	two fluorescence peaks	1141:1162	two fluorescence peaks at 433 and 511 nm	1141:1180	The spray-coated paper documents exhibit two absorbance bands at 256 and 358 nm, and two fluorescence peaks at 433 and 511 nm.
34323370	1	3	theme	polyacrylic	320:330	arg1	polymer					339:345	a polyacrylic binder polymer	318:345	a polyacrylic binder polymer	318:345	New photochromic film was developed toward the preparation of anti-counterfeiting documents utilizing inorganic/organic nanocomposite enclosing a photoluminescent inorganic pigment and a polyacrylic binder polymer.
34323370	0	4	theme	aluminate	88:96	arg1	spray-coating					50:62	spray-coating	50:62	spray-coating of rare-earth strontium aluminate for anti-counterfeit applications	50:130	Production of photochromic nanocomposite film via spray-coating of rare-earth strontium aluminate for anti-counterfeit applications.
34323370	9	5	theme	produced	1382:1389	arg1	composites					1404:1413	the produced photochromic composites	1378:1413	the produced photochromic composites	1378:1413	The rheology of the produced photochromic composites as well as the mechanical properties and photostability of the spray-coated documents were studied.
34323370	2	6	theme	aluminum	448:455	arg1	pigment					463:469	the phosphorescent strontium aluminum oxide pigment	419:469	the phosphorescent strontium aluminum oxide pigment	419:469	To generate a translucent film from pigment/polyacrylic nanocomposite, the phosphorescent strontium aluminum oxide pigment should be well-dispersed in the solution of the polyacrylic-based binder without agglomeration.
34323370	2	6	theme	aluminum	448:455	arg1	well-dispersed					481:494	well-dispersed	481:494	well-dispersed	481:494	To generate a translucent film from pigment/polyacrylic nanocomposite, the phosphorescent strontium aluminum oxide pigment should be well-dispersed in the solution of the polyacrylic-based binder without agglomeration.
34323370	2	7	from	nanocomposite	404:416	arg1	film					374:377	a translucent film	360:377	a translucent film from pigment/polyacrylic nanocomposite	360:416	To generate a translucent film from pigment/polyacrylic nanocomposite, the phosphorescent strontium aluminum oxide pigment should be well-dispersed in the solution of the polyacrylic-based binder without agglomeration.
34323370	1	8	theme	binder	332:337	arg1	polymer					339:345	a polyacrylic binder polymer	318:345	a polyacrylic binder polymer	318:345	New photochromic film was developed toward the preparation of anti-counterfeiting documents utilizing inorganic/organic nanocomposite enclosing a photoluminescent inorganic pigment and a polyacrylic binder polymer.
34323370	7	9	dep	spray-coated	1207:1218	arg1	documents					1220:1228	documents	1220:1228	documents	1220:1228	The morphologies of the spray-coated documents were explored.
34323370	6	10	theme	absorbance	1101:1110	arg1	bands					1112:1116	two absorbance bands	1097:1116	two absorbance bands at 256 and 358 nm	1097:1134	The spray-coated paper documents exhibit two absorbance bands at 256 and 358 nm, and two fluorescence peaks at 433 and 511 nm.
34323370	9	11	theme	photochromic	1391:1402	arg1	composites					1404:1413	the produced photochromic composites	1378:1413	the produced photochromic composites	1378:1413	The rheology of the produced photochromic composites as well as the mechanical properties and photostability of the spray-coated documents were studied.
34323370	2	12	theme	translucent	362:372	arg1	film					374:377	a translucent film	360:377	a translucent film from pigment/polyacrylic nanocomposite	360:416	To generate a translucent film from pigment/polyacrylic nanocomposite, the phosphorescent strontium aluminum oxide pigment should be well-dispersed in the solution of the polyacrylic-based binder without agglomeration.
34323370	4	13	theme	homogeneous	760:770	arg1	film					785:788	A homogeneous photochromic film	758:788	A homogeneous photochromic film	758:788	A homogeneous photochromic film was immobilized onto cellulose paper surface to introduce a transparent film changing to greenish-yellow upon exposure to ultraviolet light as depicted by CIE coloration measurements.
34323370	0	14	theme	anti-counterfeit	102:117	arg1	applications					119:130	anti-counterfeit applications	102:130	anti-counterfeit applications	102:130	Production of photochromic nanocomposite film via spray-coating of rare-earth strontium aluminate for anti-counterfeit applications.
34323370	3	15	theme	economical	692:701	arg1	technology					717:726	the effective and economical spray-coating technology	674:726	the effective and economical spray-coating technology followed with thermofixation	674:755	The photochromic nanocomposite was applied efficiently onto commercial cellulose paper documents utilizing the effective and economical spray-coating technology followed with thermofixation.
34323370	9	16	theme	composites	1404:1413	arg1	photostability					1456:1469	photostability	1456:1469	photostability	1456:1469	The rheology of the produced photochromic composites as well as the mechanical properties and photostability of the spray-coated documents were studied.
34323370	9	16	theme	composites	1404:1413	arg1	rheology					1366:1373	The rheology	1362:1373	The rheology of the produced photochromic composites as well as the mechanical properties and photostability of the spray-coated documents	1362:1499	The rheology of the produced photochromic composites as well as the mechanical properties and photostability of the spray-coated documents were studied.
34323370	9	16	theme	composites	1404:1413	arg1	properties					1441:1450	mechanical properties	1430:1450	mechanical properties	1430:1450	The rheology of the produced photochromic composites as well as the mechanical properties and photostability of the spray-coated documents were studied.
34323370	3	17	theme	cellulose	638:646	arg1	paper					648:652	commercial cellulose paper documents	627:662	commercial cellulose paper documents utilizing the effective and economical spray-coating technology followed with thermofixation	627:755	The photochromic nanocomposite was applied efficiently onto commercial cellulose paper documents utilizing the effective and economical spray-coating technology followed with thermofixation.
34323370	9	18	dep	spray-coated	1478:1489	arg1	documents					1491:1499	documents	1491:1499	documents	1491:1499	The rheology of the produced photochromic composites as well as the mechanical properties and photostability of the spray-coated documents were studied.
34323370	2	19	theme	strontium	438:446	arg1	pigment					463:469	the phosphorescent strontium aluminum oxide pigment	419:469	the phosphorescent strontium aluminum oxide pigment	419:469	To generate a translucent film from pigment/polyacrylic nanocomposite, the phosphorescent strontium aluminum oxide pigment should be well-dispersed in the solution of the polyacrylic-based binder without agglomeration.
34323370	2	19	theme	strontium	438:446	arg1	well-dispersed					481:494	well-dispersed	481:494	well-dispersed	481:494	To generate a translucent film from pigment/polyacrylic nanocomposite, the phosphorescent strontium aluminum oxide pigment should be well-dispersed in the solution of the polyacrylic-based binder without agglomeration.
34323370	1	20	theme	anti-counterfeiting	195:213	arg1	preparation					180:190	the preparation	176:190	the preparation of anti-counterfeiting documents utilizing inorganic/organic nanocomposite enclosing a photoluminescent inorganic pigment and a polyacrylic binder polymer	176:345	New photochromic film was developed toward the preparation of anti-counterfeiting documents utilizing inorganic/organic nanocomposite enclosing a photoluminescent inorganic pigment and a polyacrylic binder polymer.
34323370	6	21	from	433	1167:1169	arg1	bands					1112:1116	two absorbance bands	1097:1116	two absorbance bands at 256 and 358 nm	1097:1134	The spray-coated paper documents exhibit two absorbance bands at 256 and 358 nm, and two fluorescence peaks at 433 and 511 nm.
34323370	6	21	from	433	1167:1169	arg1	peaks					1158:1162	two fluorescence peaks	1141:1162	two fluorescence peaks at 433 and 511 nm	1141:1180	The spray-coated paper documents exhibit two absorbance bands at 256 and 358 nm, and two fluorescence peaks at 433 and 511 nm.
34323370	4	22	theme	cellulose	811:819	arg1	surface					827:833	cellulose paper surface	811:833	cellulose paper surface	811:833	A homogeneous photochromic film was immobilized onto cellulose paper surface to introduce a transparent film changing to greenish-yellow upon exposure to ultraviolet light as depicted by CIE coloration measurements.
34323370	3	23	theme	effective	678:686	arg1	technology					717:726	the effective and economical spray-coating technology	674:726	the effective and economical spray-coating technology followed with thermofixation	674:755	The photochromic nanocomposite was applied efficiently onto commercial cellulose paper documents utilizing the effective and economical spray-coating technology followed with thermofixation.
34323370	8	24	theme	paper	1262:1266	arg1	sheets					1268:1273	The spray-coated paper sheets	1245:1273	The spray-coated paper sheets	1245:1273	The spray-coated paper sheets showed a reversible photochromic effect without fatigue under ultraviolet irradiation.
34323370	8	25	theme	ultraviolet	1337:1347	arg1	irradiation					1349:1359	ultraviolet irradiation	1337:1359	ultraviolet irradiation	1337:1359	The spray-coated paper sheets showed a reversible photochromic effect without fatigue under ultraviolet irradiation.
34323370	4	26	theme	transparent	850:860	arg1	film					862:865	a transparent film	848:865	a transparent film changing to greenish-yellow upon exposure to ultraviolet light	848:928	A homogeneous photochromic film was immobilized onto cellulose paper surface to introduce a transparent film changing to greenish-yellow upon exposure to ultraviolet light as depicted by CIE coloration measurements.
34323370	6	27	from	511 nm	1175:1180	arg1	bands					1112:1116	two absorbance bands	1097:1116	two absorbance bands at 256 and 358 nm	1097:1134	The spray-coated paper documents exhibit two absorbance bands at 256 and 358 nm, and two fluorescence peaks at 433 and 511 nm.
34323370	6	27	from	511 nm	1175:1180	arg1	peaks					1158:1162	two fluorescence peaks	1141:1162	two fluorescence peaks at 433 and 511 nm	1141:1180	The spray-coated paper documents exhibit two absorbance bands at 256 and 358 nm, and two fluorescence peaks at 433 and 511 nm.
34323370	2	28	theme	pigment/polyacrylic	384:402	arg1	nanocomposite					404:416	pigment/polyacrylic nanocomposite	384:416	pigment/polyacrylic nanocomposite	384:416	To generate a translucent film from pigment/polyacrylic nanocomposite, the phosphorescent strontium aluminum oxide pigment should be well-dispersed in the solution of the polyacrylic-based binder without agglomeration.
34323370	1	29	theme	inorganic/organic	235:251	arg1	nanocomposite					253:265	inorganic/organic nanocomposite	235:265	inorganic/organic nanocomposite enclosing a photoluminescent inorganic pigment and a polyacrylic binder polymer	235:345	New photochromic film was developed toward the preparation of anti-counterfeiting documents utilizing inorganic/organic nanocomposite enclosing a photoluminescent inorganic pigment and a polyacrylic binder polymer.
34323370	0	30	theme	nanocomposite	27:39	arg1	film					41:44	photochromic nanocomposite film	14:44	photochromic nanocomposite film	14:44	Production of photochromic nanocomposite film via spray-coating of rare-earth strontium aluminate for anti-counterfeit applications.
34323370	9	31	theme	mechanical	1430:1439	arg1	properties					1441:1450	mechanical properties	1430:1450	mechanical properties	1430:1450	The rheology of the produced photochromic composites as well as the mechanical properties and photostability of the spray-coated documents were studied.
34323370	3	32	theme	spray-coating	703:715	arg1	technology					717:726	the effective and economical spray-coating technology	674:726	the effective and economical spray-coating technology followed with thermofixation	674:755	The photochromic nanocomposite was applied efficiently onto commercial cellulose paper documents utilizing the effective and economical spray-coating technology followed with thermofixation.
34323370	2	33	theme	polyacrylic-based	519:535	arg1	binder					537:542	the polyacrylic-based binder	515:542	the polyacrylic-based binder	515:542	To generate a translucent film from pigment/polyacrylic nanocomposite, the phosphorescent strontium aluminum oxide pigment should be well-dispersed in the solution of the polyacrylic-based binder without agglomeration.
34323370	2	34	theme	binder	537:542	arg1	solution					503:510	the solution	499:510	the solution of the polyacrylic-based binder without agglomeration	499:564	To generate a translucent film from pigment/polyacrylic nanocomposite, the phosphorescent strontium aluminum oxide pigment should be well-dispersed in the solution of the polyacrylic-based binder without agglomeration.
34323370	6	35	theme	fluorescence	1145:1156	arg1	peaks					1158:1162	two fluorescence peaks	1141:1162	two fluorescence peaks at 433 and 511 nm	1141:1180	The spray-coated paper documents exhibit two absorbance bands at 256 and 358 nm, and two fluorescence peaks at 433 and 511 nm.
34323370	6	36	theme	spray-coated	1060:1071	arg1	paper					1073:1077	The spray-coated paper	1056:1077	The spray-coated paper	1056:1077	The spray-coated paper documents exhibit two absorbance bands at 256 and 358 nm, and two fluorescence peaks at 433 and 511 nm.
34323370	1	37	theme	New	133:135	arg1	film					150:153	New photochromic film	133:153	New photochromic film	133:153	New photochromic film was developed toward the preparation of anti-counterfeiting documents utilizing inorganic/organic nanocomposite enclosing a photoluminescent inorganic pigment and a polyacrylic binder polymer.
34323370	3	38	theme	commercial	627:636	arg1	paper					648:652	commercial cellulose paper documents	627:662	commercial cellulose paper documents utilizing the effective and economical spray-coating technology followed with thermofixation	627:755	The photochromic nanocomposite was applied efficiently onto commercial cellulose paper documents utilizing the effective and economical spray-coating technology followed with thermofixation.
34323370	8	39	theme	reversible	1284:1293	arg1	effect					1308:1313	a reversible photochromic effect	1282:1313	a reversible photochromic effect	1282:1313	The spray-coated paper sheets showed a reversible photochromic effect without fatigue under ultraviolet irradiation.
34323370	4	40	theme	paper	821:825	arg1	surface					827:833	cellulose paper surface	811:833	cellulose paper surface	811:833	A homogeneous photochromic film was immobilized onto cellulose paper surface to introduce a transparent film changing to greenish-yellow upon exposure to ultraviolet light as depicted by CIE coloration measurements.
34323370	0	41	theme	film	41:44	arg1	Production					0:9	Production	0:9	Production of photochromic nanocomposite film via spray-coating of rare-earth strontium aluminate for anti-counterfeit applications.	0:131	Production of photochromic nanocomposite film via spray-coating of rare-earth strontium aluminate for anti-counterfeit applications.
34323370	5	42	theme	photochromic	978:989	arg1	effect					991:996	The photochromic effect	974:996	The photochromic effect	974:996	The photochromic effect was monitored at lowest pigment concentration (0.25 wt%).
34323370	4	43	theme	CIE	945:947	arg1	measurements					960:971	CIE coloration measurements	945:971	CIE coloration measurements	945:971	A homogeneous photochromic film was immobilized onto cellulose paper surface to introduce a transparent film changing to greenish-yellow upon exposure to ultraviolet light as depicted by CIE coloration measurements.
34323370	5	44	theme	lowest	1015:1020	arg1	concentration					1030:1042	lowest pigment concentration	1015:1042	lowest pigment concentration (0.25 wt%)	1015:1053	The photochromic effect was monitored at lowest pigment concentration (0.25 wt%).
34323370	5	44	theme	lowest	1015:1020	arg1	%					1052:1052	0.25 wt%	1045:1052	0.25 wt%	1045:1052	The photochromic effect was monitored at lowest pigment concentration (0.25 wt%).
34323370	2	45	from	well-dispersed	481:494	arg1	solution					503:510	the solution	499:510	the solution of the polyacrylic-based binder without agglomeration	499:564	To generate a translucent film from pigment/polyacrylic nanocomposite, the phosphorescent strontium aluminum oxide pigment should be well-dispersed in the solution of the polyacrylic-based binder without agglomeration.
34323370	1	46	theme	photoluminescent	279:294	arg1	pigment					306:312	a photoluminescent inorganic pigment	277:312	a photoluminescent inorganic pigment	277:312	New photochromic film was developed toward the preparation of anti-counterfeiting documents utilizing inorganic/organic nanocomposite enclosing a photoluminescent inorganic pigment and a polyacrylic binder polymer.
34323370	5	47	theme	pigment	1022:1028	arg1	concentration					1030:1042	lowest pigment concentration	1015:1042	lowest pigment concentration (0.25 wt%)	1015:1053	The photochromic effect was monitored at lowest pigment concentration (0.25 wt%).
34323370	5	47	theme	pigment	1022:1028	arg1	%					1052:1052	0.25 wt%	1045:1052	0.25 wt%	1045:1052	The photochromic effect was monitored at lowest pigment concentration (0.25 wt%).
34323370	1	48	dep	anti-counterfeiting	195:213	arg1	documents					215:223	documents	215:223	documents	215:223	New photochromic film was developed toward the preparation of anti-counterfeiting documents utilizing inorganic/organic nanocomposite enclosing a photoluminescent inorganic pigment and a polyacrylic binder polymer.
34323370	1	49	theme	photochromic	137:148	arg1	film					150:153	New photochromic film	133:153	New photochromic film	133:153	New photochromic film was developed toward the preparation of anti-counterfeiting documents utilizing inorganic/organic nanocomposite enclosing a photoluminescent inorganic pigment and a polyacrylic binder polymer.
34323370	8	50	theme	photochromic	1295:1306	arg1	effect					1308:1313	a reversible photochromic effect	1282:1313	a reversible photochromic effect	1282:1313	The spray-coated paper sheets showed a reversible photochromic effect without fatigue under ultraviolet irradiation.
34323370	3	51	dep	paper	648:652	arg1	documents					654:662	documents	654:662	documents	654:662	The photochromic nanocomposite was applied efficiently onto commercial cellulose paper documents utilizing the effective and economical spray-coating technology followed with thermofixation.
34323370	2	52	theme	phosphorescent	423:436	arg1	pigment					463:469	the phosphorescent strontium aluminum oxide pigment	419:469	the phosphorescent strontium aluminum oxide pigment	419:469	To generate a translucent film from pigment/polyacrylic nanocomposite, the phosphorescent strontium aluminum oxide pigment should be well-dispersed in the solution of the polyacrylic-based binder without agglomeration.
34323370	2	52	theme	phosphorescent	423:436	arg1	well-dispersed					481:494	well-dispersed	481:494	well-dispersed	481:494	To generate a translucent film from pigment/polyacrylic nanocomposite, the phosphorescent strontium aluminum oxide pigment should be well-dispersed in the solution of the polyacrylic-based binder without agglomeration.
34323370	8	53	theme	spray-coated	1249:1260	arg1	sheets					1268:1273	The spray-coated paper sheets	1245:1273	The spray-coated paper sheets	1245:1273	The spray-coated paper sheets showed a reversible photochromic effect without fatigue under ultraviolet irradiation.
34323370	7	54	theme	spray-coated	1207:1218	arg1	morphologies					1187:1198	The morphologies	1183:1198	The morphologies of the spray-coated documents	1183:1228	The morphologies of the spray-coated documents were explored.
34323370	9	55	theme	spray-coated	1478:1489	arg1	photostability					1456:1469	photostability	1456:1469	photostability	1456:1469	The rheology of the produced photochromic composites as well as the mechanical properties and photostability of the spray-coated documents were studied.
34323370	9	55	theme	spray-coated	1478:1489	arg1	rheology					1366:1373	The rheology	1362:1373	The rheology of the produced photochromic composites as well as the mechanical properties and photostability of the spray-coated documents	1362:1499	The rheology of the produced photochromic composites as well as the mechanical properties and photostability of the spray-coated documents were studied.
34323370	9	55	theme	spray-coated	1478:1489	arg1	properties					1441:1450	mechanical properties	1430:1450	mechanical properties	1430:1450	The rheology of the produced photochromic composites as well as the mechanical properties and photostability of the spray-coated documents were studied.
34323370	4	56	theme	ultraviolet	912:922	arg1	light					924:928	ultraviolet light	912:928	ultraviolet light	912:928	A homogeneous photochromic film was immobilized onto cellulose paper surface to introduce a transparent film changing to greenish-yellow upon exposure to ultraviolet light as depicted by CIE coloration measurements.
34323370	1	57	theme	inorganic	296:304	arg1	pigment					306:312	a photoluminescent inorganic pigment	277:312	a photoluminescent inorganic pigment	277:312	New photochromic film was developed toward the preparation of anti-counterfeiting documents utilizing inorganic/organic nanocomposite enclosing a photoluminescent inorganic pigment and a polyacrylic binder polymer.
34323370	4	58	theme	coloration	949:958	arg1	measurements					960:971	CIE coloration measurements	945:971	CIE coloration measurements	945:971	A homogeneous photochromic film was immobilized onto cellulose paper surface to introduce a transparent film changing to greenish-yellow upon exposure to ultraviolet light as depicted by CIE coloration measurements.
34323370	4	59	theme	photochromic	772:783	arg1	film					785:788	A homogeneous photochromic film	758:788	A homogeneous photochromic film	758:788	A homogeneous photochromic film was immobilized onto cellulose paper surface to introduce a transparent film changing to greenish-yellow upon exposure to ultraviolet light as depicted by CIE coloration measurements.
34323370	0	60	theme	strontium	78:86	arg1	aluminate					88:96	rare-earth strontium aluminate	67:96	rare-earth strontium aluminate for anti-counterfeit applications	67:130	Production of photochromic nanocomposite film via spray-coating of rare-earth strontium aluminate for anti-counterfeit applications.
34323370	3	61	theme	photochromic	571:582	arg1	nanocomposite					584:596	The photochromic nanocomposite	567:596	The photochromic nanocomposite	567:596	The photochromic nanocomposite was applied efficiently onto commercial cellulose paper documents utilizing the effective and economical spray-coating technology followed with thermofixation.
34323370	2	62	from	solution	503:510	arg1	pigment					463:469	the phosphorescent strontium aluminum oxide pigment	419:469	the phosphorescent strontium aluminum oxide pigment	419:469	To generate a translucent film from pigment/polyacrylic nanocomposite, the phosphorescent strontium aluminum oxide pigment should be well-dispersed in the solution of the polyacrylic-based binder without agglomeration.
34323370	2	62	from	solution	503:510	arg1	well-dispersed					481:494	well-dispersed	481:494	well-dispersed	481:494	To generate a translucent film from pigment/polyacrylic nanocomposite, the phosphorescent strontium aluminum oxide pigment should be well-dispersed in the solution of the polyacrylic-based binder without agglomeration.
34323370	9	63	dep	properties	1441:1450	arg1	the					1426:1428	the	1426:1428	the	1426:1428	The rheology of the produced photochromic composites as well as the mechanical properties and photostability of the spray-coated documents were studied.
34323370	0	64	theme	rare-earth	67:76	arg1	aluminate					88:96	rare-earth strontium aluminate	67:96	rare-earth strontium aluminate for anti-counterfeit applications	67:130	Production of photochromic nanocomposite film via spray-coating of rare-earth strontium aluminate for anti-counterfeit applications.
34323370	0	65	theme	photochromic	14:25	arg1	film					41:44	photochromic nanocomposite film	14:44	photochromic nanocomposite film	14:44	Production of photochromic nanocomposite film via spray-coating of rare-earth strontium aluminate for anti-counterfeit applications.
32610141	0	0	theme	Chaperone	100:108	arg1	N-Glycosylation					79:93	Aberrant N-Glycosylation	70:93	Aberrant N-Glycosylation of a Chaperone	70:108	Molecular Stressors Engender Protein Connectivity Dysfunction through Aberrant N-Glycosylation of a Chaperone.
32610141	6	1	theme	biochemical	935:945	arg1	evidence					947:954	biochemical evidence	935:954	biochemical evidence for stressor-induced chaperone-mediated protein mis-assemblies	935:1017	In summary, we provide biochemical evidence for stressor-induced chaperone-mediated protein mis-assemblies and demonstrate how these alterations are actionable in disease.
32610141	5	2	gly	N-glycosylated	882:895	arg1	variant					903:909	the N-glycosylated GRP94 variant	878:909	the N-glycosylated GRP94 variant	878:909	We show in cells, human specimens, and mouse xenografts that proteome connectivity is restorable by inhibition of the N-glycosylated GRP94 variant.
32610141	0	3	gly	N-Glycosylation	79:93	arg1	Chaperone					100:108	a Chaperone	98:108	a Chaperone	98:108	Molecular Stressors Engender Protein Connectivity Dysfunction through Aberrant N-Glycosylation of a Chaperone.
32610141	3	4	with	interactions	626:637	arg1	proteins					644:651	proteins	644:651	proteins at the plasma membrane	644:674	Our study unveils a specific N-glycosylation pattern used by a chaperone, Glucose-regulated protein 94 (GRP94), to alter its conformational fitness and stabilize a state most permissive for stable interactions with proteins at the plasma membrane.
32610141	4	5	theme	cell	758:761	arg1	properties					740:749	properties	740:749	properties	740:749	This "protein assembly mutation' remodels protein networks and properties of the cell.
32610141	4	5	theme	cell	758:761	arg1	networks					727:734	protein networks	719:734	protein networks	719:734	This "protein assembly mutation' remodels protein networks and properties of the cell.
32610141	2	6	theme	important	332:340	arg1	role					342:345	an important role	329:345	an important role	329:345	Chaperones play an important role in these alterations, but how these changes are executed remains largely unknown.
32610141	5	7	theme	N-glycosylated	882:895	arg1	variant					903:909	the N-glycosylated GRP94 variant	878:909	the N-glycosylated GRP94 variant	878:909	We show in cells, human specimens, and mouse xenografts that proteome connectivity is restorable by inhibition of the N-glycosylated GRP94 variant.
32610141	1	8	theme	interaction	203:213	arg1	strength					215:222	interaction strength	203:222	interaction strength	203:222	Stresses associated with disease may pathologically remodel the proteome by both increasing interaction strength and altering interaction partners, resulting in proteome-wide connectivity dysfunctions.
32610141	5	9	theme	proteome	825:832	arg1	connectivity					834:845	proteome connectivity	825:845	proteome connectivity	825:845	We show in cells, human specimens, and mouse xenografts that proteome connectivity is restorable by inhibition of the N-glycosylated GRP94 variant.
32610141	3	10	theme	permissive	604:613	arg1	state					593:597	a state	591:597	a state most permissive for stable interactions with proteins at the plasma membrane	591:674	Our study unveils a specific N-glycosylation pattern used by a chaperone, Glucose-regulated protein 94 (GRP94), to alter its conformational fitness and stabilize a state most permissive for stable interactions with proteins at the plasma membrane.
32610141	3	11	from	membrane	667:674	arg1	proteins					644:651	proteins	644:651	proteins at the plasma membrane	644:674	Our study unveils a specific N-glycosylation pattern used by a chaperone, Glucose-regulated protein 94 (GRP94), to alter its conformational fitness and stabilize a state most permissive for stable interactions with proteins at the plasma membrane.
32610141	5	12	theme	variant	903:909	arg1	inhibition					864:873	inhibition	864:873	inhibition of the N-glycosylated GRP94 variant	864:909	We show in cells, human specimens, and mouse xenografts that proteome connectivity is restorable by inhibition of the N-glycosylated GRP94 variant.
32610141	0	13	theme	Molecular	0:8	arg1	Stressors					10:18	Molecular Stressors	0:18	Molecular Stressors	0:18	Molecular Stressors Engender Protein Connectivity Dysfunction through Aberrant N-Glycosylation of a Chaperone.
32610141	6	14	from	actionable	1061:1070	arg1	disease					1075:1081	disease	1075:1081	disease	1075:1081	In summary, we provide biochemical evidence for stressor-induced chaperone-mediated protein mis-assemblies and demonstrate how these alterations are actionable in disease.
32610141	5	15	theme	GRP94	897:901	arg1	variant					903:909	the N-glycosylated GRP94 variant	878:909	the N-glycosylated GRP94 variant	878:909	We show in cells, human specimens, and mouse xenografts that proteome connectivity is restorable by inhibition of the N-glycosylated GRP94 variant.
32610141	6	16	from	disease	1075:1081	arg1	actionable					1061:1070	actionable	1061:1070	actionable	1061:1070	In summary, we provide biochemical evidence for stressor-induced chaperone-mediated protein mis-assemblies and demonstrate how these alterations are actionable in disease.
32610141	0	17	theme	Protein	29:35	arg1	Dysfunction					50:60	Protein Connectivity Dysfunction	29:60	Protein Connectivity Dysfunction	29:60	Molecular Stressors Engender Protein Connectivity Dysfunction through Aberrant N-Glycosylation of a Chaperone.
32610141	1	18	theme	interaction	237:247	arg1	partners					249:256	interaction partners	237:256	interaction partners	237:256	Stresses associated with disease may pathologically remodel the proteome by both increasing interaction strength and altering interaction partners, resulting in proteome-wide connectivity dysfunctions.
32610141	3	19	theme	N-glycosylation	458:472	arg1	pattern					474:480	a specific N-glycosylation pattern	447:480	a specific N-glycosylation pattern used by a chaperone, Glucose-regulated protein 94 (GRP94), to alter its conformational fitness and stabilize a state most permissive for stable interactions with proteins at the plasma membrane	447:674	Our study unveils a specific N-glycosylation pattern used by a chaperone, Glucose-regulated protein 94 (GRP94), to alter its conformational fitness and stabilize a state most permissive for stable interactions with proteins at the plasma membrane.
32610141	4	20	theme	protein	683:689	arg1	mutation					700:707	This "protein assembly mutation'	677:708	This "protein assembly mutation'	677:708	This "protein assembly mutation' remodels protein networks and properties of the cell.
32610141	0	21	theme	Connectivity	37:48	arg1	Dysfunction					50:60	Protein Connectivity Dysfunction	29:60	Protein Connectivity Dysfunction	29:60	Molecular Stressors Engender Protein Connectivity Dysfunction through Aberrant N-Glycosylation of a Chaperone.
32610141	5	22	theme	mouse	803:807	arg1	xenografts					809:818	mouse xenografts	803:818	mouse xenografts	803:818	We show in cells, human specimens, and mouse xenografts that proteome connectivity is restorable by inhibition of the N-glycosylated GRP94 variant.
32610141	3	23	theme	Glucose-regulated	503:519	arg1	chaperone					492:500	a chaperone	490:500	a chaperone	490:500	Our study unveils a specific N-glycosylation pattern used by a chaperone, Glucose-regulated protein 94 (GRP94), to alter its conformational fitness and stabilize a state most permissive for stable interactions with proteins at the plasma membrane.
32610141	3	23	theme	Glucose-regulated	503:519	arg1	protein					521:527	Glucose-regulated protein 94	503:530	Glucose-regulated protein 94 (GRP94)	503:538	Our study unveils a specific N-glycosylation pattern used by a chaperone, Glucose-regulated protein 94 (GRP94), to alter its conformational fitness and stabilize a state most permissive for stable interactions with proteins at the plasma membrane.
32610141	3	23	theme	Glucose-regulated	503:519	arg1	GRP94					533:537	GRP94	533:537	GRP94	533:537	Our study unveils a specific N-glycosylation pattern used by a chaperone, Glucose-regulated protein 94 (GRP94), to alter its conformational fitness and stabilize a state most permissive for stable interactions with proteins at the plasma membrane.
32610141	0	24	theme	Aberrant	70:77	arg1	N-Glycosylation					79:93	Aberrant N-Glycosylation	70:93	Aberrant N-Glycosylation of a Chaperone	70:108	Molecular Stressors Engender Protein Connectivity Dysfunction through Aberrant N-Glycosylation of a Chaperone.
32610141	6	25	theme	protein	996:1002	arg1	mis-assemblies					1004:1017	stressor-induced chaperone-mediated protein mis-assemblies	960:1017	stressor-induced chaperone-mediated protein mis-assemblies	960:1017	In summary, we provide biochemical evidence for stressor-induced chaperone-mediated protein mis-assemblies and demonstrate how these alterations are actionable in disease.
32610141	3	26	theme	stable	619:624	arg1	interactions					626:637	stable interactions	619:637	stable interactions with proteins at the plasma membrane	619:674	Our study unveils a specific N-glycosylation pattern used by a chaperone, Glucose-regulated protein 94 (GRP94), to alter its conformational fitness and stabilize a state most permissive for stable interactions with proteins at the plasma membrane.
32610141	3	27	theme	specific	449:456	arg1	pattern					474:480	a specific N-glycosylation pattern	447:480	a specific N-glycosylation pattern used by a chaperone, Glucose-regulated protein 94 (GRP94), to alter its conformational fitness and stabilize a state most permissive for stable interactions with proteins at the plasma membrane	447:674	Our study unveils a specific N-glycosylation pattern used by a chaperone, Glucose-regulated protein 94 (GRP94), to alter its conformational fitness and stabilize a state most permissive for stable interactions with proteins at the plasma membrane.
32610141	6	28	theme	chaperone-mediated	977:994	arg1	mis-assemblies					1004:1017	stressor-induced chaperone-mediated protein mis-assemblies	960:1017	stressor-induced chaperone-mediated protein mis-assemblies	960:1017	In summary, we provide biochemical evidence for stressor-induced chaperone-mediated protein mis-assemblies and demonstrate how these alterations are actionable in disease.
32610141	6	29	theme	stressor-induced	960:975	arg1	mis-assemblies					1004:1017	stressor-induced chaperone-mediated protein mis-assemblies	960:1017	stressor-induced chaperone-mediated protein mis-assemblies	960:1017	In summary, we provide biochemical evidence for stressor-induced chaperone-mediated protein mis-assemblies and demonstrate how these alterations are actionable in disease.
32610141	3	30	theme	plasma	660:665	arg1	membrane					667:674	the plasma membrane	656:674	the plasma membrane	656:674	Our study unveils a specific N-glycosylation pattern used by a chaperone, Glucose-regulated protein 94 (GRP94), to alter its conformational fitness and stabilize a state most permissive for stable interactions with proteins at the plasma membrane.
32610141	3	31	theme	conformational	554:567	arg1	fitness					569:575	its conformational fitness	550:575	its conformational fitness	550:575	Our study unveils a specific N-glycosylation pattern used by a chaperone, Glucose-regulated protein 94 (GRP94), to alter its conformational fitness and stabilize a state most permissive for stable interactions with proteins at the plasma membrane.
32610141	4	32	theme	assembly	691:698	arg1	mutation					700:707	This "protein assembly mutation'	677:708	This "protein assembly mutation'	677:708	This "protein assembly mutation' remodels protein networks and properties of the cell.
32610141	1	33	theme	proteome-wide	272:284	arg1	dysfunctions					299:310	proteome-wide connectivity dysfunctions	272:310	proteome-wide connectivity dysfunctions	272:310	Stresses associated with disease may pathologically remodel the proteome by both increasing interaction strength and altering interaction partners, resulting in proteome-wide connectivity dysfunctions.
32610141	5	34	theme	human	782:786	arg1	specimens					788:796	human specimens	782:796	human specimens	782:796	We show in cells, human specimens, and mouse xenografts that proteome connectivity is restorable by inhibition of the N-glycosylated GRP94 variant.
32610141	4	35	theme	protein	719:725	arg1	networks					727:734	protein networks	719:734	protein networks	719:734	This "protein assembly mutation' remodels protein networks and properties of the cell.
32610141	1	36	theme	connectivity	286:297	arg1	dysfunctions					299:310	proteome-wide connectivity dysfunctions	272:310	proteome-wide connectivity dysfunctions	272:310	Stresses associated with disease may pathologically remodel the proteome by both increasing interaction strength and altering interaction partners, resulting in proteome-wide connectivity dysfunctions.
34954417	6	0	theme	CP	845:846	arg1	motifs					813:818	Rebers and Riddiford motifs	792:818	Rebers and Riddiford motifs (CPR)	792:824	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	6	0	theme	CP	845:846	arg1	family					848:853	the most abundant CP family	827:853	the most abundant CP family	827:853	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	7	1	theme	elytral	1154:1160	arg1	macro					1162:1166	elytral macro	1154:1166	elytral macro	1154:1166	These protein groups may play crucial roles in elytra formation at different time points, likely including self-assembly of chitin nanofibers that control elytral macro and microstructures and dictate changes in other properties (i.e., mechanical property).
34954417	11	2	theme	architectural	1837:1849	arg1	characteristics					1877:1891	proteomic, architectural, and localized mechanical characteristics	1826:1891	proteomic, architectural, and localized mechanical characteristics of elytra	1826:1901	This work, which involves insights from molecular biology and materials science, investigated changes in proteomic, architectural, and localized mechanical characteristics of elytra from the Japanese rhinoceros beetle to understand molecular mechanisms driving elytra development.
34954417	12	3	theme	green	2181:2185	arg1	materials					2187:2195	processing green materials	2170:2195	processing green materials	2170:2195	In the present study, we identified a set of new protein groups which are likely related to the structural development of elytra and has potential for new pathways for processing green materials.
34954417	5	4	theme	untanned	635:642	arg1	elytra					655:660	both untanned and tanned elytra	630:660	both untanned and tanned elytra	630:660	Using mass spectrometry, a total of 414 proteins were identified from both untanned and tanned elytra, including 31 cuticular proteins (CPs), which constitute one of the major components of insect cuticles.
34954417	12	5	theme	structural	2098:2107	arg1	development					2109:2119	the structural development	2094:2119	the structural development of elytra	2094:2129	In the present study, we identified a set of new protein groups which are likely related to the structural development of elytra and has potential for new pathways for processing green materials.
34954417	5	6	theme	414	596:598	arg1	proteins					600:607	414 proteins	596:607	414 proteins	596:607	Using mass spectrometry, a total of 414 proteins were identified from both untanned and tanned elytra, including 31 cuticular proteins (CPs), which constitute one of the major components of insect cuticles.
34954417	11	7	theme	molecular	1761:1769	arg1	biology					1771:1777	molecular biology	1761:1777	molecular biology	1761:1777	This work, which involves insights from molecular biology and materials science, investigated changes in proteomic, architectural, and localized mechanical characteristics of elytra from the Japanese rhinoceros beetle to understand molecular mechanisms driving elytra development.
34954417	11	8	from	beetle	1932:1937	arg1	changes					1815:1821	changes	1815:1821	changes in proteomic, architectural, and localized mechanical characteristics of elytra from the Japanese rhinoceros beetle	1815:1937	This work, which involves insights from molecular biology and materials science, investigated changes in proteomic, architectural, and localized mechanical characteristics of elytra from the Japanese rhinoceros beetle to understand molecular mechanisms driving elytra development.
34954417	12	9	theme	processing	2170:2179	arg1	materials					2187:2195	processing green materials	2170:2195	processing green materials	2170:2195	In the present study, we identified a set of new protein groups which are likely related to the structural development of elytra and has potential for new pathways for processing green materials.
34954417	4	10	from	changes	422:428	arg1	composition					441:451	protein composition	433:451	protein composition	433:451	Here, we identified changes in protein composition during the developmental processes of the elytra in the Japanese rhinoceros beetle, Trypoxylus dichotomus.
34954417	7	11	theme	different	1066:1074	arg1	points					1081:1086	different time points	1066:1086	different time points	1066:1086	These protein groups may play crucial roles in elytra formation at different time points, likely including self-assembly of chitin nanofibers that control elytral macro and microstructures and dictate changes in other properties (i.e., mechanical property).
34954417	12	12	theme	protein	2051:2057	arg1	groups					2059:2064	new protein groups	2047:2064	new protein groups	2047:2064	In the present study, we identified a set of new protein groups which are likely related to the structural development of elytra and has potential for new pathways for processing green materials.
34954417	5	13	theme	tanned	648:653	arg1	elytra					655:660	both untanned and tanned elytra	630:660	both untanned and tanned elytra	630:660	Using mass spectrometry, a total of 414 proteins were identified from both untanned and tanned elytra, including 31 cuticular proteins (CPs), which constitute one of the major components of insect cuticles.
34954417	1	14	theme	modified	115:122	arg1	lightweight					164:174	lightweight	164:174	lightweight	164:174	Beetles possess a set of highly modified and tanned forewings, elytra, which are lightweight yet rigid and tough.
34954417	1	14	theme	modified	115:122	arg1	elytra					146:151	elytra	146:151	elytra	146:151	Beetles possess a set of highly modified and tanned forewings, elytra, which are lightweight yet rigid and tough.
34954417	1	14	theme	modified	115:122	arg1	set					101:103	a set	99:103	a set of highly modified and tanned forewings	99:143	Beetles possess a set of highly modified and tanned forewings, elytra, which are lightweight yet rigid and tough.
34954417	1	14	theme	modified	115:122	arg1	modified					115:122	modified	115:122	modified	115:122	Beetles possess a set of highly modified and tanned forewings, elytra, which are lightweight yet rigid and tough.
34954417	1	14	theme	modified	115:122	arg1	forewings					135:143	tanned forewings	128:143	tanned forewings	128:143	Beetles possess a set of highly modified and tanned forewings, elytra, which are lightweight yet rigid and tough.
34954417	8	15	theme	materials	1402:1410	arg1	development					1375:1385	the development	1371:1385	the development of lightweight materials for industrial and biomedical applications	1371:1453	Clarification of the protein functions will enhance the understanding of elytra formation and potentially benefit the development of lightweight materials for industrial and biomedical applications.
34954417	11	16	theme	elytra	1982:1987	arg1	development					1989:1999	elytra development	1982:1999	elytra development	1982:1999	This work, which involves insights from molecular biology and materials science, investigated changes in proteomic, architectural, and localized mechanical characteristics of elytra from the Japanese rhinoceros beetle to understand molecular mechanisms driving elytra development.
34954417	1	17	theme	tanned	128:133	arg1	forewings					135:143	tanned forewings	128:143	tanned forewings	128:143	Beetles possess a set of highly modified and tanned forewings, elytra, which are lightweight yet rigid and tough.
34954417	8	18	theme	industrial	1416:1425	arg1	applications					1442:1453	industrial and biomedical applications	1416:1453	industrial and biomedical applications	1416:1453	Clarification of the protein functions will enhance the understanding of elytra formation and potentially benefit the development of lightweight materials for industrial and biomedical applications.
34954417	0	19	theme	beetle	68:73	arg1	elytra					75:80	beetle elytra	68:80	beetle elytra	68:80	Unveiling characteristic proteins for the structural development of beetle elytra.
34954417	9	20	theme	SIGNIFICANCE	1469:1480	arg1	STATEMENT					1456:1464	STATEMENT	1456:1464	STATEMENT OF SIGNIFICANCE: The beetle elytron	1456:1500	STATEMENT OF SIGNIFICANCE: The beetle elytron is a light-weight natural bio-composite which displays high stiffness and toughness.
34954417	9	20	theme	SIGNIFICANCE	1469:1480	arg1	bio-composite					1528:1540	a light-weight natural bio-composite	1505:1540	a light-weight natural bio-composite which displays high stiffness and toughness	1505:1584	STATEMENT OF SIGNIFICANCE: The beetle elytron is a light-weight natural bio-composite which displays high stiffness and toughness.
34954417	6	21	theme	acid	923:926	arg1	sequences					928:936	their expression and amino acid sequences	896:936	sequences	928:936	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	6	21	theme	acid	923:926	arg1	region					967:972	a Gly-rich sequence region	947:972	a Gly-rich sequence region	947:972	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	6	21	theme	acid	923:926	arg1	repeats					990:996	Ala-Ala-Pro repeats	978:996	Ala-Ala-Pro repeats	978:996	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	4	22	theme	developmental	464:476	arg1	processes					478:486	the developmental processes	460:486	the developmental processes of the elytra in the Japanese rhinoceros beetle	460:534	Here, we identified changes in protein composition during the developmental processes of the elytra in the Japanese rhinoceros beetle, Trypoxylus dichotomus.
34954417	6	23	contain	containing	781:790	arg2	family					848:853	the most abundant CP family	827:853	the most abundant CP family	827:853	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	6	23	contain	containing	781:790	arg2	CPR					821:823	CPR	821:823	CPR	821:823	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	6	23	contain	containing	781:790	arg2	motifs					813:818	Rebers and Riddiford motifs	792:818	Rebers and Riddiford motifs (CPR)	792:824	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	6	23	contain	containing	781:790	arg1	CPs					777:779	CPs	777:779	CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family,	777:854	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	7	24	theme	nanofibers	1130:1139	arg1	self-assembly					1106:1118	self-assembly	1106:1118	self-assembly of chitin nanofibers that control elytral macro and microstructures and dictate changes in other properties (i.e., mechanical property)	1106:1254	These protein groups may play crucial roles in elytra formation at different time points, likely including self-assembly of chitin nanofibers that control elytral macro and microstructures and dictate changes in other properties (i.e., mechanical property).
34954417	4	25	theme	protein	433:439	arg1	composition					441:451	protein composition	433:451	protein composition	433:451	Here, we identified changes in protein composition during the developmental processes of the elytra in the Japanese rhinoceros beetle, Trypoxylus dichotomus.
34954417	10	26	theme	chitin	1617:1622	arg1	fibrils					1624:1630	chitin fibrils	1617:1630	chitin fibrils	1617:1630	This structure is composed of chitin fibrils and proteins, some of which are responsible for architectural development and hardening.
34954417	6	27	theme	Ala-Ala-Pro	978:988	arg1	repeats					990:996	Ala-Ala-Pro repeats	978:996	Ala-Ala-Pro repeats	978:996	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	12	28	contain	has	2135:2137	arg1	set					2040:2042	a set	2038:2042	a set of new protein groups which are likely related to the structural development of elytra and has potential for new pathways for processing green materials	2038:2195	In the present study, we identified a set of new protein groups which are likely related to the structural development of elytra and has potential for new pathways for processing green materials.
34954417	12	28	contain	has	2135:2137	arg1	groups					2059:2064	new protein groups	2047:2064	new protein groups	2047:2064	In the present study, we identified a set of new protein groups which are likely related to the structural development of elytra and has potential for new pathways for processing green materials.
34954417	12	28	contain	has	2135:2137	arg1	related					2083:2089	related	2083:2089	related	2083:2089	In the present study, we identified a set of new protein groups which are likely related to the structural development of elytra and has potential for new pathways for processing green materials.
34954417	12	28	contain	has	2135:2137	arg2	potential					2139:2147	potential	2139:2147	potential for new pathways for processing green materials	2139:2195	In the present study, we identified a set of new protein groups which are likely related to the structural development of elytra and has potential for new pathways for processing green materials.
34954417	11	29	from	changes	1815:1821	arg1	characteristics					1877:1891	proteomic, architectural, and localized mechanical characteristics	1826:1891	proteomic, architectural, and localized mechanical characteristics of elytra	1826:1901	This work, which involves insights from molecular biology and materials science, investigated changes in proteomic, architectural, and localized mechanical characteristics of elytra from the Japanese rhinoceros beetle to understand molecular mechanisms driving elytra development.
34954417	8	30	theme	functions	1286:1294	arg1	Clarification					1257:1269	Clarification	1257:1269	Clarification of the protein functions	1257:1294	Clarification of the protein functions will enhance the understanding of elytra formation and potentially benefit the development of lightweight materials for industrial and biomedical applications.
34954417	11	31	theme	localized	1856:1864	arg1	characteristics					1877:1891	proteomic, architectural, and localized mechanical characteristics	1826:1891	proteomic, architectural, and localized mechanical characteristics of elytra	1826:1901	This work, which involves insights from molecular biology and materials science, investigated changes in proteomic, architectural, and localized mechanical characteristics of elytra from the Japanese rhinoceros beetle to understand molecular mechanisms driving elytra development.
34954417	5	32	theme	cuticles	757:764	arg1	components					736:745	the major components	726:745	the major components of insect cuticles	726:764	Using mass spectrometry, a total of 414 proteins were identified from both untanned and tanned elytra, including 31 cuticular proteins (CPs), which constitute one of the major components of insect cuticles.
34954417	9	33	theme	natural	1520:1526	arg1	STATEMENT					1456:1464	STATEMENT	1456:1464	STATEMENT OF SIGNIFICANCE: The beetle elytron	1456:1500	STATEMENT OF SIGNIFICANCE: The beetle elytron is a light-weight natural bio-composite which displays high stiffness and toughness.
34954417	9	33	theme	natural	1520:1526	arg1	bio-composite					1528:1540	a light-weight natural bio-composite	1505:1540	a light-weight natural bio-composite which displays high stiffness and toughness	1505:1584	STATEMENT OF SIGNIFICANCE: The beetle elytron is a light-weight natural bio-composite which displays high stiffness and toughness.
34954417	7	34	theme	mechanical	1235:1244	arg1	property					1246:1253	mechanical property	1235:1253	mechanical property	1235:1253	These protein groups may play crucial roles in elytra formation at different time points, likely including self-assembly of chitin nanofibers that control elytral macro and microstructures and dictate changes in other properties (i.e., mechanical property).
34954417	4	35	theme	rhinoceros	518:527	arg1	beetle					529:534	the Japanese rhinoceros beetle	505:534	the Japanese rhinoceros beetle	505:534	Here, we identified changes in protein composition during the developmental processes of the elytra in the Japanese rhinoceros beetle, Trypoxylus dichotomus.
34954417	7	36	theme	time	1076:1079	arg1	points					1081:1086	different time points	1066:1086	different time points	1066:1086	These protein groups may play crucial roles in elytra formation at different time points, likely including self-assembly of chitin nanofibers that control elytral macro and microstructures and dictate changes in other properties (i.e., mechanical property).
34954417	6	37	theme	Gly-rich	949:956	arg1	region					967:972	a Gly-rich sequence region	947:972	a Gly-rich sequence region	947:972	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	12	38	theme	new	2153:2155	arg1	pathways					2157:2164	new pathways	2153:2164	new pathways	2153:2164	In the present study, we identified a set of new protein groups which are likely related to the structural development of elytra and has potential for new pathways for processing green materials.
34954417	11	39	theme	elytra	1896:1901	arg1	characteristics					1877:1891	proteomic, architectural, and localized mechanical characteristics	1826:1891	proteomic, architectural, and localized mechanical characteristics of elytra	1826:1901	This work, which involves insights from molecular biology and materials science, investigated changes in proteomic, architectural, and localized mechanical characteristics of elytra from the Japanese rhinoceros beetle to understand molecular mechanisms driving elytra development.
34954417	5	40	theme	major	730:734	arg1	components					736:745	the major components	726:745	the major components of insect cuticles	726:764	Using mass spectrometry, a total of 414 proteins were identified from both untanned and tanned elytra, including 31 cuticular proteins (CPs), which constitute one of the major components of insect cuticles.
34954417	6	41	theme	Riddiford	803:811	arg1	CPR					821:823	CPR	821:823	CPR	821:823	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	6	41	theme	Riddiford	803:811	arg1	motifs					813:818	Rebers and Riddiford motifs	792:818	Rebers and Riddiford motifs (CPR)	792:824	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	6	41	theme	Riddiford	803:811	arg1	family					848:853	the most abundant CP family	827:853	the most abundant CP family	827:853	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	9	42	theme	high	1557:1560	arg1	stiffness					1562:1570	high stiffness	1557:1570	high stiffness	1557:1570	STATEMENT OF SIGNIFICANCE: The beetle elytron is a light-weight natural bio-composite which displays high stiffness and toughness.
34954417	4	43	theme	elytra	495:500	arg1	processes					478:486	the developmental processes	460:486	the developmental processes of the elytra in the Japanese rhinoceros beetle	460:534	Here, we identified changes in protein composition during the developmental processes of the elytra in the Japanese rhinoceros beetle, Trypoxylus dichotomus.
34954417	8	44	theme	elytra	1330:1335	arg1	formation					1337:1345	elytra formation	1330:1345	elytra formation	1330:1345	Clarification of the protein functions will enhance the understanding of elytra formation and potentially benefit the development of lightweight materials for industrial and biomedical applications.
34954417	7	45	theme	elytra	1046:1051	arg1	formation					1053:1061	elytra formation	1046:1061	elytra formation at different time points	1046:1086	These protein groups may play crucial roles in elytra formation at different time points, likely including self-assembly of chitin nanofibers that control elytral macro and microstructures and dictate changes in other properties (i.e., mechanical property).
34954417	6	46	theme	Rebers	792:797	arg1	CPR					821:823	CPR	821:823	CPR	821:823	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	6	46	theme	Rebers	792:797	arg1	motifs					813:818	Rebers and Riddiford motifs	792:818	Rebers and Riddiford motifs (CPR)	792:824	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	6	46	theme	Rebers	792:797	arg1	family					848:853	the most abundant CP family	827:853	the most abundant CP family	827:853	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	12	47	theme	present	2009:2015	arg1	study					2017:2021	the present study	2005:2021	the present study	2005:2021	In the present study, we identified a set of new protein groups which are likely related to the structural development of elytra and has potential for new pathways for processing green materials.
34954417	7	48	theme	crucial	1029:1035	arg1	roles					1037:1041	crucial roles	1029:1041	crucial roles	1029:1041	These protein groups may play crucial roles in elytra formation at different time points, likely including self-assembly of chitin nanofibers that control elytral macro and microstructures and dictate changes in other properties (i.e., mechanical property).
34954417	8	49	theme	biomedical	1431:1440	arg1	applications					1442:1453	industrial and biomedical applications	1416:1453	industrial and biomedical applications	1416:1453	Clarification of the protein functions will enhance the understanding of elytra formation and potentially benefit the development of lightweight materials for industrial and biomedical applications.
34954417	6	50	theme	abundant	836:843	arg1	motifs					813:818	Rebers and Riddiford motifs	792:818	Rebers and Riddiford motifs (CPR)	792:824	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	6	50	theme	abundant	836:843	arg1	family					848:853	the most abundant CP family	827:853	the most abundant CP family	827:853	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	11	51	from	materials	1783:1791	arg1	insights					1747:1754	insights	1747:1754	insights from molecular biology and materials science	1747:1799	This work, which involves insights from molecular biology and materials science, investigated changes in proteomic, architectural, and localized mechanical characteristics of elytra from the Japanese rhinoceros beetle to understand molecular mechanisms driving elytra development.
34954417	4	52	from	processes	478:486	arg1	beetle					529:534	the Japanese rhinoceros beetle	505:534	the Japanese rhinoceros beetle	505:534	Here, we identified changes in protein composition during the developmental processes of the elytra in the Japanese rhinoceros beetle, Trypoxylus dichotomus.
34954417	7	53	theme	protein	1005:1011	arg1	groups					1013:1018	These protein groups	999:1018	These protein groups	999:1018	These protein groups may play crucial roles in elytra formation at different time points, likely including self-assembly of chitin nanofibers that control elytral macro and microstructures and dictate changes in other properties (i.e., mechanical property).
34954417	5	54	theme	insect	750:755	arg1	cuticles					757:764	insect cuticles	750:764	insect cuticles	750:764	Using mass spectrometry, a total of 414 proteins were identified from both untanned and tanned elytra, including 31 cuticular proteins (CPs), which constitute one of the major components of insect cuticles.
34954417	4	55	from	elytra	495:500	arg1	beetle					529:534	the Japanese rhinoceros beetle	505:534	the Japanese rhinoceros beetle	505:534	Here, we identified changes in protein composition during the developmental processes of the elytra in the Japanese rhinoceros beetle, Trypoxylus dichotomus.
34954417	5	56	theme	proteins	600:607	arg1	total					587:591	a total	585:591	a total of 414 proteins	585:607	Using mass spectrometry, a total of 414 proteins were identified from both untanned and tanned elytra, including 31 cuticular proteins (CPs), which constitute one of the major components of insect cuticles.
34954417	11	57	from	biology	1771:1777	arg1	insights					1747:1754	insights	1747:1754	insights from molecular biology and materials science	1747:1799	This work, which involves insights from molecular biology and materials science, investigated changes in proteomic, architectural, and localized mechanical characteristics of elytra from the Japanese rhinoceros beetle to understand molecular mechanisms driving elytra development.
34954417	7	58	from	changes	1200:1206	arg1	properties					1217:1226	other properties	1211:1226	other properties (i.e., mechanical property)	1211:1254	These protein groups may play crucial roles in elytra formation at different time points, likely including self-assembly of chitin nanofibers that control elytral macro and microstructures and dictate changes in other properties (i.e., mechanical property).
34954417	5	59	theme	cuticular	676:684	arg1	CPs					696:698	CPs	696:698	CPs	696:698	Using mass spectrometry, a total of 414 proteins were identified from both untanned and tanned elytra, including 31 cuticular proteins (CPs), which constitute one of the major components of insect cuticles.
34954417	5	59	theme	cuticular	676:684	arg1	proteins					686:693	31 cuticular proteins	673:693	31 cuticular proteins (CPs)	673:699	Using mass spectrometry, a total of 414 proteins were identified from both untanned and tanned elytra, including 31 cuticular proteins (CPs), which constitute one of the major components of insect cuticles.
34954417	8	60	theme	lightweight	1390:1400	arg1	materials					1402:1410	lightweight materials	1390:1410	lightweight materials for industrial and biomedical applications	1390:1453	Clarification of the protein functions will enhance the understanding of elytra formation and potentially benefit the development of lightweight materials for industrial and biomedical applications.
34954417	11	61	theme	rhinoceros	1921:1930	arg1	beetle					1932:1937	the Japanese rhinoceros beetle	1908:1937	the Japanese rhinoceros beetle	1908:1937	This work, which involves insights from molecular biology and materials science, investigated changes in proteomic, architectural, and localized mechanical characteristics of elytra from the Japanese rhinoceros beetle to understand molecular mechanisms driving elytra development.
34954417	12	62	theme	new	2047:2049	arg1	groups					2059:2064	new protein groups	2047:2064	new protein groups	2047:2064	In the present study, we identified a set of new protein groups which are likely related to the structural development of elytra and has potential for new pathways for processing green materials.
34954417	1	63	contain	possess	91:97	arg2	modified					115:122	modified	115:122	modified	115:122	Beetles possess a set of highly modified and tanned forewings, elytra, which are lightweight yet rigid and tough.
34954417	1	63	contain	possess	91:97	arg2	set					101:103	a set	99:103	a set of highly modified and tanned forewings	99:143	Beetles possess a set of highly modified and tanned forewings, elytra, which are lightweight yet rigid and tough.
34954417	1	63	contain	possess	91:97	arg1	Beetles					83:89	Beetles	83:89	Beetles	83:89	Beetles possess a set of highly modified and tanned forewings, elytra, which are lightweight yet rigid and tough.
34954417	1	63	contain	possess	91:97	arg2	forewings					135:143	tanned forewings	128:143	tanned forewings	128:143	Beetles possess a set of highly modified and tanned forewings, elytra, which are lightweight yet rigid and tough.
34954417	1	63	contain	possess	91:97	arg2	lightweight					164:174	lightweight	164:174	lightweight	164:174	Beetles possess a set of highly modified and tanned forewings, elytra, which are lightweight yet rigid and tough.
34954417	1	63	contain	possess	91:97	arg2	elytra					146:151	elytra	146:151	elytra	146:151	Beetles possess a set of highly modified and tanned forewings, elytra, which are lightweight yet rigid and tough.
34954417	12	64	theme	elytra	2124:2129	arg1	development					2109:2119	the structural development	2094:2119	the structural development of elytra	2094:2129	In the present study, we identified a set of new protein groups which are likely related to the structural development of elytra and has potential for new pathways for processing green materials.
34954417	12	65	theme	groups	2059:2064	arg1	set					2040:2042	a set	2038:2042	a set of new protein groups which are likely related to the structural development of elytra and has potential for new pathways for processing green materials	2038:2195	In the present study, we identified a set of new protein groups which are likely related to the structural development of elytra and has potential for new pathways for processing green materials.
34954417	12	65	theme	groups	2059:2064	arg1	related					2083:2089	related	2083:2089	related	2083:2089	In the present study, we identified a set of new protein groups which are likely related to the structural development of elytra and has potential for new pathways for processing green materials.
34954417	12	65	theme	groups	2059:2064	arg1	groups					2059:2064	new protein groups	2047:2064	new protein groups	2047:2064	In the present study, we identified a set of new protein groups which are likely related to the structural development of elytra and has potential for new pathways for processing green materials.
34954417	0	66	theme	elytra	75:80	arg1	development					53:63	the structural development	38:63	the structural development of beetle elytra	38:80	Unveiling characteristic proteins for the structural development of beetle elytra.
34954417	11	67	theme	molecular	1953:1961	arg1	mechanisms					1963:1972	molecular mechanisms	1953:1972	molecular mechanisms driving elytra development	1953:1999	This work, which involves insights from molecular biology and materials science, investigated changes in proteomic, architectural, and localized mechanical characteristics of elytra from the Japanese rhinoceros beetle to understand molecular mechanisms driving elytra development.
34954417	1	68	theme	forewings	135:143	arg1	lightweight					164:174	lightweight	164:174	lightweight	164:174	Beetles possess a set of highly modified and tanned forewings, elytra, which are lightweight yet rigid and tough.
34954417	1	68	theme	forewings	135:143	arg1	elytra					146:151	elytra	146:151	elytra	146:151	Beetles possess a set of highly modified and tanned forewings, elytra, which are lightweight yet rigid and tough.
34954417	1	68	theme	forewings	135:143	arg1	set					101:103	a set	99:103	a set of highly modified and tanned forewings	99:143	Beetles possess a set of highly modified and tanned forewings, elytra, which are lightweight yet rigid and tough.
34954417	1	68	theme	forewings	135:143	arg1	modified					115:122	modified	115:122	modified	115:122	Beetles possess a set of highly modified and tanned forewings, elytra, which are lightweight yet rigid and tough.
34954417	1	68	theme	forewings	135:143	arg1	forewings					135:143	tanned forewings	128:143	tanned forewings	128:143	Beetles possess a set of highly modified and tanned forewings, elytra, which are lightweight yet rigid and tough.
34954417	7	69	theme	chitin	1123:1128	arg1	nanofibers					1130:1139	chitin nanofibers	1123:1139	chitin nanofibers that control elytral macro and microstructures and dictate changes in other properties (i.e., mechanical property)	1123:1254	These protein groups may play crucial roles in elytra formation at different time points, likely including self-assembly of chitin nanofibers that control elytral macro and microstructures and dictate changes in other properties (i.e., mechanical property).
34954417	6	70	theme	amino	917:921	arg1	sequences					928:936	their expression and amino acid sequences	896:936	sequences	928:936	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	6	70	theme	amino	917:921	arg1	region					967:972	a Gly-rich sequence region	947:972	a Gly-rich sequence region	947:972	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	6	70	theme	amino	917:921	arg1	repeats					990:996	Ala-Ala-Pro repeats	978:996	Ala-Ala-Pro repeats	978:996	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	11	71	dep	biology	1771:1777	arg1	science					1793:1799	science	1793:1799	science	1793:1799	This work, which involves insights from molecular biology and materials science, investigated changes in proteomic, architectural, and localized mechanical characteristics of elytra from the Japanese rhinoceros beetle to understand molecular mechanisms driving elytra development.
34954417	9	72	theme	beetle	1487:1492	arg1	elytron					1494:1500	The beetle elytron	1483:1500	STATEMENT OF SIGNIFICANCE: The beetle elytron	1456:1500	STATEMENT OF SIGNIFICANCE: The beetle elytron is a light-weight natural bio-composite which displays high stiffness and toughness.
34954417	9	73	dep	STATEMENT	1456:1464	arg1	elytron					1494:1500	The beetle elytron	1483:1500	STATEMENT OF SIGNIFICANCE: The beetle elytron	1456:1500	STATEMENT OF SIGNIFICANCE: The beetle elytron is a light-weight natural bio-composite which displays high stiffness and toughness.
34954417	7	74	from	points	1081:1086	arg1	formation					1053:1061	elytra formation	1046:1061	elytra formation at different time points	1046:1086	These protein groups may play crucial roles in elytra formation at different time points, likely including self-assembly of chitin nanofibers that control elytral macro and microstructures and dictate changes in other properties (i.e., mechanical property).
34954417	0	75	theme	characteristic	10:23	arg1	proteins					25:32	characteristic proteins	10:32	characteristic proteins	10:32	Unveiling characteristic proteins for the structural development of beetle elytra.
34954417	9	76	theme	light-weight	1507:1518	arg1	STATEMENT					1456:1464	STATEMENT	1456:1464	STATEMENT OF SIGNIFICANCE: The beetle elytron	1456:1500	STATEMENT OF SIGNIFICANCE: The beetle elytron is a light-weight natural bio-composite which displays high stiffness and toughness.
34954417	9	76	theme	light-weight	1507:1518	arg1	bio-composite					1528:1540	a light-weight natural bio-composite	1505:1540	a light-weight natural bio-composite which displays high stiffness and toughness	1505:1584	STATEMENT OF SIGNIFICANCE: The beetle elytron is a light-weight natural bio-composite which displays high stiffness and toughness.
34954417	8	77	theme	protein	1278:1284	arg1	functions					1286:1294	the protein functions	1274:1294	the protein functions	1274:1294	Clarification of the protein functions will enhance the understanding of elytra formation and potentially benefit the development of lightweight materials for industrial and biomedical applications.
34954417	11	78	theme	mechanical	1866:1875	arg1	characteristics					1877:1891	proteomic, architectural, and localized mechanical characteristics	1826:1891	proteomic, architectural, and localized mechanical characteristics of elytra	1826:1901	This work, which involves insights from molecular biology and materials science, investigated changes in proteomic, architectural, and localized mechanical characteristics of elytra from the Japanese rhinoceros beetle to understand molecular mechanisms driving elytra development.
34954417	4	79	from	beetle	529:534	arg1	processes					478:486	the developmental processes	460:486	the developmental processes of the elytra in the Japanese rhinoceros beetle	460:534	Here, we identified changes in protein composition during the developmental processes of the elytra in the Japanese rhinoceros beetle, Trypoxylus dichotomus.
34954417	5	80	theme	mass	566:569	arg1	spectrometry					571:582	mass spectrometry	566:582	mass spectrometry	566:582	Using mass spectrometry, a total of 414 proteins were identified from both untanned and tanned elytra, including 31 cuticular proteins (CPs), which constitute one of the major components of insect cuticles.
34954417	6	81	theme	sequence	958:965	arg1	region					967:972	a Gly-rich sequence region	947:972	a Gly-rich sequence region	947:972	Moreover, CPs containing Rebers and Riddiford motifs (CPR), the most abundant CP family, were separated into two groups based on their expression and amino acid sequences, such as a Gly-rich sequence region and Ala-Ala-Pro repeats.
34954417	11	82	theme	Japanese	1912:1919	arg1	beetle					1932:1937	the Japanese rhinoceros beetle	1908:1937	the Japanese rhinoceros beetle	1908:1937	This work, which involves insights from molecular biology and materials science, investigated changes in proteomic, architectural, and localized mechanical characteristics of elytra from the Japanese rhinoceros beetle to understand molecular mechanisms driving elytra development.
34954417	0	83	theme	structural	42:51	arg1	development					53:63	the structural development	38:63	the structural development of beetle elytra	38:80	Unveiling characteristic proteins for the structural development of beetle elytra.
34954417	7	84	theme	other	1211:1215	arg1	properties					1217:1226	other properties	1211:1226	other properties (i.e., mechanical property)	1211:1254	These protein groups may play crucial roles in elytra formation at different time points, likely including self-assembly of chitin nanofibers that control elytral macro and microstructures and dictate changes in other properties (i.e., mechanical property).
34954417	5	85	theme	components	736:745	arg1	components					736:745	the major components	726:745	the major components of insect cuticles	726:764	Using mass spectrometry, a total of 414 proteins were identified from both untanned and tanned elytra, including 31 cuticular proteins (CPs), which constitute one of the major components of insect cuticles.
34954417	5	85	theme	components	736:745	arg1	one					719:721	one	719:721	one	719:721	Using mass spectrometry, a total of 414 proteins were identified from both untanned and tanned elytra, including 31 cuticular proteins (CPs), which constitute one of the major components of insect cuticles.
34954417	7	86	dep	property	1246:1253	arg1	i.e.					1229:1232	i.e.	1229:1232	i.e.	1229:1232	These protein groups may play crucial roles in elytra formation at different time points, likely including self-assembly of chitin nanofibers that control elytral macro and microstructures and dictate changes in other properties (i.e., mechanical property).
34954417	4	87	theme	Japanese	509:516	arg1	beetle					529:534	the Japanese rhinoceros beetle	505:534	the Japanese rhinoceros beetle	505:534	Here, we identified changes in protein composition during the developmental processes of the elytra in the Japanese rhinoceros beetle, Trypoxylus dichotomus.
34954417	11	88	theme	proteomic	1826:1834	arg1	characteristics					1877:1891	proteomic, architectural, and localized mechanical characteristics	1826:1891	proteomic, architectural, and localized mechanical characteristics of elytra	1826:1901	This work, which involves insights from molecular biology and materials science, investigated changes in proteomic, architectural, and localized mechanical characteristics of elytra from the Japanese rhinoceros beetle to understand molecular mechanisms driving elytra development.
34954417	8	89	theme	formation	1337:1345	arg1	understanding					1313:1325	the understanding	1309:1325	the understanding of elytra formation	1309:1345	Clarification of the protein functions will enhance the understanding of elytra formation and potentially benefit the development of lightweight materials for industrial and biomedical applications.
34954417	10	90	theme	architectural	1680:1692	arg1	development					1694:1704	development	1694:1704	development	1694:1704	This structure is composed of chitin fibrils and proteins, some of which are responsible for architectural development and hardening.
33120266	2	0	theme	cell	415:418	arg1	wall					420:423	cell wall	415:423	cell wall	415:423	Nitric oxide (NO) plays an important role in plant growth and responses to abiotic stresses, however, it is not clear whether NO is connected with the response of cell wall to aluminum (Al) tolerance in rice (Oryza sativa L.).
33120266	5	1	theme	SNP	914:916	arg1	addition					902:909	The addition	898:909	The addition of SNP	898:916	The addition of SNP significantly decreased Al-induced accumulation of pectin, hemicellulose 1 and hemicellulose 2 by 43.1%, 13.1% and 19.2% in Zhefu802 and by 16.9%, 13.4% and 14.0% in Nipponbare, compared with roots treated with Al alone, as well as pectin methylesterase (PME) activity.
33120266	7	2	theme	blue	1593:1596	arg1	Evans					1587:1591	Evans blue	1587:1596	Evans blue	1587:1596	Furthermore, the activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) treated by Al were all increased by SNP pretreatment, and the lipid peroxidation and damage to plasma membrane of root tips detected with Schiff's reagent and Evans blue reduced.
33120266	1	3	theme	cell	150:153	arg1	barrier					184:190	barrier	184:190	barrier	184:190	Plant cell wall, the first interface or barrier for toxic ions entering into protoplast, suffers from risk.
33120266	1	3	theme	cell	150:153	arg1	interface					171:179	the first interface	161:179	the first interface	161:179	Plant cell wall, the first interface or barrier for toxic ions entering into protoplast, suffers from risk.
33120266	1	3	theme	cell	150:153	arg1	wall					155:158	Plant cell wall	144:158	Plant cell wall	144:158	Plant cell wall, the first interface or barrier for toxic ions entering into protoplast, suffers from risk.
33120266	5	4	theme	hemicellulose	997:1009	arg1	accumulation					953:964	Al-induced accumulation	942:964	Al-induced accumulation of pectin, hemicellulose 1 and hemicellulose 2	942:1011	The addition of SNP significantly decreased Al-induced accumulation of pectin, hemicellulose 1 and hemicellulose 2 by 43.1%, 13.1% and 19.2% in Zhefu802 and by 16.9%, 13.4% and 14.0% in Nipponbare, compared with roots treated with Al alone, as well as pectin methylesterase (PME) activity.
33120266	4	5	theme	Al-induced	744:753	arg1	inhibition					755:764	Al-induced inhibition	744:764	Al-induced inhibition of root elongation by 32.3% and 91.7%	744:802	Pretreatment with 100 µM NO donor (sodium nitroprusside, SNP) reduced Al-induced inhibition of root elongation by 32.3% and 91.7%, and Al accumulation in root-tip by 38.4% and 44.3% in Nipponbare and Zhefu802, respectively.
33120266	3	6	theme	reductase	552:560	arg1	activity					567:574	nitrate reductase (NR) activity	544:574	nitrate reductase (NR) activity	544:574	In this study, we found that the application of 50 µM Al induces nitrate reductase (NR) activity and endogenous NO production, but not nitric oxide synthase (NOS) activity in two rice genotypes.
33120266	9	7	theme	walls	1836:1840	arg1	structure					1818:1826	the structure	1814:1826	the structure of cell walls	1814:1840	These results indicated that by regulating the Al-binding capacity to cell walls and lipid peroxidation, the structure of cell walls can be stabilized and that Al toxicity in rice can be alleviated with increased NO.
33120266	0	8	theme	cell	87:90	arg1	composition					97:107	the cell wall composition	83:107	the cell wall composition	83:107	Nitric oxide reduces the aluminum-binding capacity in rice root tips by regulating the cell wall composition and enhancing antioxidant enzymes.
33120266	9	9	theme	increased	1912:1920	arg1	NO					1922:1923	increased NO	1912:1923	increased NO	1912:1923	These results indicated that by regulating the Al-binding capacity to cell walls and lipid peroxidation, the structure of cell walls can be stabilized and that Al toxicity in rice can be alleviated with increased NO.
33120266	5	10	theme	pectin	1150:1155	arg1	PME					1173:1175	PME	1173:1175	PME	1173:1175	The addition of SNP significantly decreased Al-induced accumulation of pectin, hemicellulose 1 and hemicellulose 2 by 43.1%, 13.1% and 19.2% in Zhefu802 and by 16.9%, 13.4% and 14.0% in Nipponbare, compared with roots treated with Al alone, as well as pectin methylesterase (PME) activity.
33120266	5	10	theme	pectin	1150:1155	arg1	methylesterase					1157:1170	pectin methylesterase	1150:1170	pectin methylesterase (PME) activity	1150:1185	The addition of SNP significantly decreased Al-induced accumulation of pectin, hemicellulose 1 and hemicellulose 2 by 43.1%, 13.1% and 19.2% in Zhefu802 and by 16.9%, 13.4% and 14.0% in Nipponbare, compared with roots treated with Al alone, as well as pectin methylesterase (PME) activity.
33120266	6	11	theme	cell	1229:1232	arg1	walls					1234:1238	cell walls	1229:1238	cell walls	1229:1238	Therefore, the content of Al absorbed in cell walls was decreased, indicating that the Al-induced structure damage to cell walls was alleviated.
33120266	4	12	with	Pretreatment	674:685	arg1	donor					702:706	100 µM NO donor	692:706	100 µM NO donor (sodium nitroprusside, SNP)	692:734	Pretreatment with 100 µM NO donor (sodium nitroprusside, SNP) reduced Al-induced inhibition of root elongation by 32.3% and 91.7%, and Al accumulation in root-tip by 38.4% and 44.3% in Nipponbare and Zhefu802, respectively.
33120266	4	12	with	Pretreatment	674:685	arg1	nitroprusside					716:728	sodium nitroprusside	709:728	sodium nitroprusside	709:728	Pretreatment with 100 µM NO donor (sodium nitroprusside, SNP) reduced Al-induced inhibition of root elongation by 32.3% and 91.7%, and Al accumulation in root-tip by 38.4% and 44.3% in Nipponbare and Zhefu802, respectively.
33120266	9	13	from	toxicity	1872:1879	arg1	rice					1884:1887	rice	1884:1887	rice	1884:1887	These results indicated that by regulating the Al-binding capacity to cell walls and lipid peroxidation, the structure of cell walls can be stabilized and that Al toxicity in rice can be alleviated with increased NO.
33120266	4	14	theme	elongation	774:783	arg1	accumulation					812:823	Al accumulation	809:823	Al accumulation in root-tip	809:835	Pretreatment with 100 µM NO donor (sodium nitroprusside, SNP) reduced Al-induced inhibition of root elongation by 32.3% and 91.7%, and Al accumulation in root-tip by 38.4% and 44.3% in Nipponbare and Zhefu802, respectively.
33120266	4	14	theme	elongation	774:783	arg1	inhibition					755:764	Al-induced inhibition	744:764	Al-induced inhibition of root elongation by 32.3% and 91.7%	744:802	Pretreatment with 100 µM NO donor (sodium nitroprusside, SNP) reduced Al-induced inhibition of root elongation by 32.3% and 91.7%, and Al accumulation in root-tip by 38.4% and 44.3% in Nipponbare and Zhefu802, respectively.
33120266	3	15	theme	endogenous	580:589	arg1	production					594:603	endogenous NO production	580:603	endogenous NO production	580:603	In this study, we found that the application of 50 µM Al induces nitrate reductase (NR) activity and endogenous NO production, but not nitric oxide synthase (NOS) activity in two rice genotypes.
33120266	4	16	theme	sodium	709:714	arg1	donor					702:706	100 µM NO donor	692:706	100 µM NO donor (sodium nitroprusside, SNP)	692:734	Pretreatment with 100 µM NO donor (sodium nitroprusside, SNP) reduced Al-induced inhibition of root elongation by 32.3% and 91.7%, and Al accumulation in root-tip by 38.4% and 44.3% in Nipponbare and Zhefu802, respectively.
33120266	4	16	theme	sodium	709:714	arg1	nitroprusside					716:728	sodium nitroprusside	709:728	sodium nitroprusside	709:728	Pretreatment with 100 µM NO donor (sodium nitroprusside, SNP) reduced Al-induced inhibition of root elongation by 32.3% and 91.7%, and Al accumulation in root-tip by 38.4% and 44.3% in Nipponbare and Zhefu802, respectively.
33120266	5	17	theme	methylesterase	1157:1170	arg1	activity					1178:1185	pectin methylesterase (PME) activity	1150:1185	pectin methylesterase (PME) activity	1150:1185	The addition of SNP significantly decreased Al-induced accumulation of pectin, hemicellulose 1 and hemicellulose 2 by 43.1%, 13.1% and 19.2% in Zhefu802 and by 16.9%, 13.4% and 14.0% in Nipponbare, compared with roots treated with Al alone, as well as pectin methylesterase (PME) activity.
33120266	3	18	theme	rice	658:661	arg1	genotypes					663:671	two rice genotypes	654:671	two rice genotypes	654:671	In this study, we found that the application of 50 µM Al induces nitrate reductase (NR) activity and endogenous NO production, but not nitric oxide synthase (NOS) activity in two rice genotypes.
33120266	3	19	theme	NO	591:592	arg1	production					594:603	endogenous NO production	580:603	endogenous NO production	580:603	In this study, we found that the application of 50 µM Al induces nitrate reductase (NR) activity and endogenous NO production, but not nitric oxide synthase (NOS) activity in two rice genotypes.
33120266	2	20	theme	Nitric	252:257	arg1	oxide					259:263	Nitric oxide	252:263	Nitric oxide (NO)	252:268	Nitric oxide (NO) plays an important role in plant growth and responses to abiotic stresses, however, it is not clear whether NO is connected with the response of cell wall to aluminum (Al) tolerance in rice (Oryza sativa L.).
33120266	2	20	theme	Nitric	252:257	arg1	NO					266:267	NO	266:267	NO	266:267	Nitric oxide (NO) plays an important role in plant growth and responses to abiotic stresses, however, it is not clear whether NO is connected with the response of cell wall to aluminum (Al) tolerance in rice (Oryza sativa L.).
33120266	7	21	theme	superoxide	1382:1391	arg1	SOD					1404:1406	SOD	1404:1406	SOD	1404:1406	Furthermore, the activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) treated by Al were all increased by SNP pretreatment, and the lipid peroxidation and damage to plasma membrane of root tips detected with Schiff's reagent and Evans blue reduced.
33120266	7	21	theme	superoxide	1382:1391	arg1	peroxidase					1364:1373	peroxidase	1364:1373	peroxidase (POD)	1364:1379	Furthermore, the activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) treated by Al were all increased by SNP pretreatment, and the lipid peroxidation and damage to plasma membrane of root tips detected with Schiff's reagent and Evans blue reduced.
33120266	7	21	theme	superoxide	1382:1391	arg1	dismutase					1393:1401	superoxide dismutase	1382:1401	superoxide dismutase (SOD)	1382:1407	Furthermore, the activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) treated by Al were all increased by SNP pretreatment, and the lipid peroxidation and damage to plasma membrane of root tips detected with Schiff's reagent and Evans blue reduced.
33120266	0	22	theme	wall	92:95	arg1	composition					97:107	the cell wall composition	83:107	the cell wall composition	83:107	Nitric oxide reduces the aluminum-binding capacity in rice root tips by regulating the cell wall composition and enhancing antioxidant enzymes.
33120266	0	23	from	capacity	42:49	arg1	tips					64:67	rice root tips	54:67	rice root tips	54:67	Nitric oxide reduces the aluminum-binding capacity in rice root tips by regulating the cell wall composition and enhancing antioxidant enzymes.
33120266	4	24	dep	nitroprusside	716:728	arg1	SNP					731:733	SNP	731:733	SNP	731:733	Pretreatment with 100 µM NO donor (sodium nitroprusside, SNP) reduced Al-induced inhibition of root elongation by 32.3% and 91.7%, and Al accumulation in root-tip by 38.4% and 44.3% in Nipponbare and Zhefu802, respectively.
33120266	7	25	dep	peroxidation	1496:1507	arg1	the					1486:1488	the	1486:1488	the	1486:1488	Furthermore, the activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) treated by Al were all increased by SNP pretreatment, and the lipid peroxidation and damage to plasma membrane of root tips detected with Schiff's reagent and Evans blue reduced.
33120266	2	26	dep	rice	455:458	arg1	Oryza					461:465	Oryza	461:465	Oryza	461:465	Nitric oxide (NO) plays an important role in plant growth and responses to abiotic stresses, however, it is not clear whether NO is connected with the response of cell wall to aluminum (Al) tolerance in rice (Oryza sativa L.).
33120266	8	27	theme	NO	1650:1651	arg1	scavenger					1653:1661	NO scavenger	1650:1661	NO scavenger (cPTIO)	1650:1669	In contrast, the effect was abolished when NO scavenger (cPTIO), and NR inhibitor (NaN3), were added.
33120266	8	27	theme	NO	1650:1651	arg1	cPTIO					1664:1668	cPTIO	1664:1668	cPTIO	1664:1668	In contrast, the effect was abolished when NO scavenger (cPTIO), and NR inhibitor (NaN3), were added.
33120266	9	28	theme	cell	1779:1782	arg1	walls					1784:1788	cell walls	1779:1788	cell walls	1779:1788	These results indicated that by regulating the Al-binding capacity to cell walls and lipid peroxidation, the structure of cell walls can be stabilized and that Al toxicity in rice can be alleviated with increased NO.
33120266	4	29	theme	NO	699:700	arg1	donor					702:706	100 µM NO donor	692:706	100 µM NO donor (sodium nitroprusside, SNP)	692:734	Pretreatment with 100 µM NO donor (sodium nitroprusside, SNP) reduced Al-induced inhibition of root elongation by 32.3% and 91.7%, and Al accumulation in root-tip by 38.4% and 44.3% in Nipponbare and Zhefu802, respectively.
33120266	4	29	theme	NO	699:700	arg1	nitroprusside					716:728	sodium nitroprusside	709:728	sodium nitroprusside	709:728	Pretreatment with 100 µM NO donor (sodium nitroprusside, SNP) reduced Al-induced inhibition of root elongation by 32.3% and 91.7%, and Al accumulation in root-tip by 38.4% and 44.3% in Nipponbare and Zhefu802, respectively.
33120266	9	30	theme	Al-binding	1756:1765	arg1	capacity					1767:1774	the Al-binding capacity	1752:1774	the Al-binding capacity to cell walls and lipid peroxidation	1752:1811	These results indicated that by regulating the Al-binding capacity to cell walls and lipid peroxidation, the structure of cell walls can be stabilized and that Al toxicity in rice can be alleviated with increased NO.
33120266	5	31	theme	pectin	969:974	arg1	accumulation					953:964	Al-induced accumulation	942:964	Al-induced accumulation of pectin, hemicellulose 1 and hemicellulose 2	942:1011	The addition of SNP significantly decreased Al-induced accumulation of pectin, hemicellulose 1 and hemicellulose 2 by 43.1%, 13.1% and 19.2% in Zhefu802 and by 16.9%, 13.4% and 14.0% in Nipponbare, compared with roots treated with Al alone, as well as pectin methylesterase (PME) activity.
33120266	1	32	theme	first	165:169	arg1	interface					171:179	the first interface	161:179	the first interface	161:179	Plant cell wall, the first interface or barrier for toxic ions entering into protoplast, suffers from risk.
33120266	1	32	theme	first	165:169	arg1	wall					155:158	Plant cell wall	144:158	Plant cell wall	144:158	Plant cell wall, the first interface or barrier for toxic ions entering into protoplast, suffers from risk.
33120266	0	33	theme	Nitric	0:5	arg1	oxide					7:11	Nitric oxide	0:11	Nitric oxide	0:11	Nitric oxide reduces the aluminum-binding capacity in rice root tips by regulating the cell wall composition and enhancing antioxidant enzymes.
33120266	4	34	from	%	844:844	arg1	Zhefu802					874:881	Zhefu802	874:881	Zhefu802	874:881	Pretreatment with 100 µM NO donor (sodium nitroprusside, SNP) reduced Al-induced inhibition of root elongation by 32.3% and 91.7%, and Al accumulation in root-tip by 38.4% and 44.3% in Nipponbare and Zhefu802, respectively.
33120266	4	34	from	%	844:844	arg1	Nipponbare					859:868	Nipponbare	859:868	Nipponbare	859:868	Pretreatment with 100 µM NO donor (sodium nitroprusside, SNP) reduced Al-induced inhibition of root elongation by 32.3% and 91.7%, and Al accumulation in root-tip by 38.4% and 44.3% in Nipponbare and Zhefu802, respectively.
33120266	3	35	theme	Al	533:534	arg1	application					512:522	the application	508:522	the application of 50 µM Al	508:534	In this study, we found that the application of 50 µM Al induces nitrate reductase (NR) activity and endogenous NO production, but not nitric oxide synthase (NOS) activity in two rice genotypes.
33120266	7	36	theme	peroxidase	1364:1373	arg1	activities					1350:1359	the activities	1346:1359	the activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) treated by Al	1346:1440	Furthermore, the activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) treated by Al were all increased by SNP pretreatment, and the lipid peroxidation and damage to plasma membrane of root tips detected with Schiff's reagent and Evans blue reduced.
33120266	2	37	theme	abiotic	327:333	arg1	stresses					335:342	abiotic stresses	327:342	abiotic stresses	327:342	Nitric oxide (NO) plays an important role in plant growth and responses to abiotic stresses, however, it is not clear whether NO is connected with the response of cell wall to aluminum (Al) tolerance in rice (Oryza sativa L.).
33120266	5	38	theme	hemicellulose	977:989	arg1	accumulation					953:964	Al-induced accumulation	942:964	Al-induced accumulation of pectin, hemicellulose 1 and hemicellulose 2	942:1011	The addition of SNP significantly decreased Al-induced accumulation of pectin, hemicellulose 1 and hemicellulose 2 by 43.1%, 13.1% and 19.2% in Zhefu802 and by 16.9%, 13.4% and 14.0% in Nipponbare, compared with roots treated with Al alone, as well as pectin methylesterase (PME) activity.
33120266	7	39	theme	tips	1547:1550	arg1	damage					1513:1518	damage	1513:1518	damage	1513:1518	Furthermore, the activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) treated by Al were all increased by SNP pretreatment, and the lipid peroxidation and damage to plasma membrane of root tips detected with Schiff's reagent and Evans blue reduced.
33120266	7	39	theme	tips	1547:1550	arg1	peroxidation					1496:1507	lipid peroxidation	1490:1507	lipid peroxidation	1490:1507	Furthermore, the activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) treated by Al were all increased by SNP pretreatment, and the lipid peroxidation and damage to plasma membrane of root tips detected with Schiff's reagent and Evans blue reduced.
33120266	6	40	theme	cell	1306:1309	arg1	walls					1311:1315	cell walls	1306:1315	cell walls	1306:1315	Therefore, the content of Al absorbed in cell walls was decreased, indicating that the Al-induced structure damage to cell walls was alleviated.
33120266	6	41	theme	absorbed	1217:1224	arg1	content					1203:1209	the content	1199:1209	the content of Al absorbed in cell walls	1199:1238	Therefore, the content of Al absorbed in cell walls was decreased, indicating that the Al-induced structure damage to cell walls was alleviated.
33120266	2	42	dep	aluminum	428:435	arg1	Al					438:439	Al	438:439	Al	438:439	Nitric oxide (NO) plays an important role in plant growth and responses to abiotic stresses, however, it is not clear whether NO is connected with the response of cell wall to aluminum (Al) tolerance in rice (Oryza sativa L.).
33120266	6	43	theme	Al	1214:1215	arg1	absorbed					1217:1224	Al absorbed	1214:1224	Al absorbed	1214:1224	Therefore, the content of Al absorbed in cell walls was decreased, indicating that the Al-induced structure damage to cell walls was alleviated.
33120266	4	44	theme	Al	809:810	arg1	accumulation					812:823	Al accumulation	809:823	Al accumulation in root-tip	809:835	Pretreatment with 100 µM NO donor (sodium nitroprusside, SNP) reduced Al-induced inhibition of root elongation by 32.3% and 91.7%, and Al accumulation in root-tip by 38.4% and 44.3% in Nipponbare and Zhefu802, respectively.
33120266	0	45	theme	root	59:62	arg1	tips					64:67	rice root tips	54:67	rice root tips	54:67	Nitric oxide reduces the aluminum-binding capacity in rice root tips by regulating the cell wall composition and enhancing antioxidant enzymes.
33120266	4	46	theme	root	769:772	arg1	elongation					774:783	root elongation	769:783	root elongation	769:783	Pretreatment with 100 µM NO donor (sodium nitroprusside, SNP) reduced Al-induced inhibition of root elongation by 32.3% and 91.7%, and Al accumulation in root-tip by 38.4% and 44.3% in Nipponbare and Zhefu802, respectively.
33120266	6	47	from	content	1203:1209	arg1	walls					1234:1238	cell walls	1229:1238	cell walls	1229:1238	Therefore, the content of Al absorbed in cell walls was decreased, indicating that the Al-induced structure damage to cell walls was alleviated.
33120266	4	48	from	%	854:854	arg1	Zhefu802					874:881	Zhefu802	874:881	Zhefu802	874:881	Pretreatment with 100 µM NO donor (sodium nitroprusside, SNP) reduced Al-induced inhibition of root elongation by 32.3% and 91.7%, and Al accumulation in root-tip by 38.4% and 44.3% in Nipponbare and Zhefu802, respectively.
33120266	4	48	from	%	854:854	arg1	Nipponbare					859:868	Nipponbare	859:868	Nipponbare	859:868	Pretreatment with 100 µM NO donor (sodium nitroprusside, SNP) reduced Al-induced inhibition of root elongation by 32.3% and 91.7%, and Al accumulation in root-tip by 38.4% and 44.3% in Nipponbare and Zhefu802, respectively.
33120266	9	49	theme	Al	1869:1870	arg1	toxicity					1872:1879	Al toxicity	1869:1879	Al toxicity in rice	1869:1887	These results indicated that by regulating the Al-binding capacity to cell walls and lipid peroxidation, the structure of cell walls can be stabilized and that Al toxicity in rice can be alleviated with increased NO.
33120266	0	50	theme	aluminum-binding	25:40	arg1	capacity					42:49	the aluminum-binding capacity	21:49	the aluminum-binding capacity in rice root tips	21:67	Nitric oxide reduces the aluminum-binding capacity in rice root tips by regulating the cell wall composition and enhancing antioxidant enzymes.
33120266	3	51	theme	synthase	627:634	arg1	activity					642:649	nitric oxide synthase (NOS) activity	614:649	nitric oxide synthase (NOS) activity	614:649	In this study, we found that the application of 50 µM Al induces nitrate reductase (NR) activity and endogenous NO production, but not nitric oxide synthase (NOS) activity in two rice genotypes.
33120266	0	52	theme	antioxidant	123:133	arg1	enzymes					135:141	antioxidant enzymes	123:141	antioxidant enzymes	123:141	Nitric oxide reduces the aluminum-binding capacity in rice root tips by regulating the cell wall composition and enhancing antioxidant enzymes.
33120266	4	53	from	inhibition	755:764	arg1	root-tip					828:835	root-tip	828:835	root-tip	828:835	Pretreatment with 100 µM NO donor (sodium nitroprusside, SNP) reduced Al-induced inhibition of root elongation by 32.3% and 91.7%, and Al accumulation in root-tip by 38.4% and 44.3% in Nipponbare and Zhefu802, respectively.
33120266	5	54	from	%	1069:1069	arg1	Nipponbare					1084:1093	Nipponbare	1084:1093	Nipponbare	1084:1093	The addition of SNP significantly decreased Al-induced accumulation of pectin, hemicellulose 1 and hemicellulose 2 by 43.1%, 13.1% and 19.2% in Zhefu802 and by 16.9%, 13.4% and 14.0% in Nipponbare, compared with roots treated with Al alone, as well as pectin methylesterase (PME) activity.
33120266	2	55	theme	aluminum	428:435	arg1	tolerance					442:450	aluminum (Al) tolerance	428:450	aluminum (Al) tolerance in rice (Oryza sativa L.)	428:476	Nitric oxide (NO) plays an important role in plant growth and responses to abiotic stresses, however, it is not clear whether NO is connected with the response of cell wall to aluminum (Al) tolerance in rice (Oryza sativa L.).
33120266	0	56	theme	rice	54:57	arg1	tips					64:67	rice root tips	54:67	rice root tips	54:67	Nitric oxide reduces the aluminum-binding capacity in rice root tips by regulating the cell wall composition and enhancing antioxidant enzymes.
33120266	4	57	from	accumulation	812:823	arg1	root-tip					828:835	root-tip	828:835	root-tip	828:835	Pretreatment with 100 µM NO donor (sodium nitroprusside, SNP) reduced Al-induced inhibition of root elongation by 32.3% and 91.7%, and Al accumulation in root-tip by 38.4% and 44.3% in Nipponbare and Zhefu802, respectively.
33120266	7	58	theme	plasma	1523:1528	arg1	membrane					1530:1537	plasma membrane	1523:1537	plasma membrane	1523:1537	Furthermore, the activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) treated by Al were all increased by SNP pretreatment, and the lipid peroxidation and damage to plasma membrane of root tips detected with Schiff's reagent and Evans blue reduced.
33120266	2	59	theme	wall	420:423	arg1	response					403:410	the response	399:410	the response of cell wall to aluminum (Al) tolerance in rice (Oryza sativa L.)	399:476	Nitric oxide (NO) plays an important role in plant growth and responses to abiotic stresses, however, it is not clear whether NO is connected with the response of cell wall to aluminum (Al) tolerance in rice (Oryza sativa L.).
33120266	5	60	from	%	1062:1062	arg1	Nipponbare					1084:1093	Nipponbare	1084:1093	Nipponbare	1084:1093	The addition of SNP significantly decreased Al-induced accumulation of pectin, hemicellulose 1 and hemicellulose 2 by 43.1%, 13.1% and 19.2% in Zhefu802 and by 16.9%, 13.4% and 14.0% in Nipponbare, compared with roots treated with Al alone, as well as pectin methylesterase (PME) activity.
33120266	2	61	theme	plant	297:301	arg1	growth					303:308	plant growth	297:308	plant growth	297:308	Nitric oxide (NO) plays an important role in plant growth and responses to abiotic stresses, however, it is not clear whether NO is connected with the response of cell wall to aluminum (Al) tolerance in rice (Oryza sativa L.).
33120266	7	62	theme	root	1542:1545	arg1	tips					1547:1550	root tips	1542:1550	root tips detected with Schiff's reagent and Evans blue	1542:1596	Furthermore, the activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) treated by Al were all increased by SNP pretreatment, and the lipid peroxidation and damage to plasma membrane of root tips detected with Schiff's reagent and Evans blue reduced.
33120266	3	63	theme	nitric	614:619	arg1	NOS					637:639	NOS	637:639	NOS	637:639	In this study, we found that the application of 50 µM Al induces nitrate reductase (NR) activity and endogenous NO production, but not nitric oxide synthase (NOS) activity in two rice genotypes.
33120266	3	63	theme	nitric	614:619	arg1	synthase					627:634	nitric oxide synthase	614:634	nitric oxide synthase (NOS) activity	614:649	In this study, we found that the application of 50 µM Al induces nitrate reductase (NR) activity and endogenous NO production, but not nitric oxide synthase (NOS) activity in two rice genotypes.
33120266	5	64	theme	Al-induced	942:951	arg1	accumulation					953:964	Al-induced accumulation	942:964	Al-induced accumulation of pectin, hemicellulose 1 and hemicellulose 2	942:1011	The addition of SNP significantly decreased Al-induced accumulation of pectin, hemicellulose 1 and hemicellulose 2 by 43.1%, 13.1% and 19.2% in Zhefu802 and by 16.9%, 13.4% and 14.0% in Nipponbare, compared with roots treated with Al alone, as well as pectin methylesterase (PME) activity.
33120266	3	65	theme	oxide	621:625	arg1	NOS					637:639	NOS	637:639	NOS	637:639	In this study, we found that the application of 50 µM Al induces nitrate reductase (NR) activity and endogenous NO production, but not nitric oxide synthase (NOS) activity in two rice genotypes.
33120266	3	65	theme	oxide	621:625	arg1	synthase					627:634	nitric oxide synthase	614:634	nitric oxide synthase (NOS) activity	614:649	In this study, we found that the application of 50 µM Al induces nitrate reductase (NR) activity and endogenous NO production, but not nitric oxide synthase (NOS) activity in two rice genotypes.
33120266	5	66	from	%	1020:1020	arg1	Zhefu802					1042:1049	Zhefu802	1042:1049	Zhefu802	1042:1049	The addition of SNP significantly decreased Al-induced accumulation of pectin, hemicellulose 1 and hemicellulose 2 by 43.1%, 13.1% and 19.2% in Zhefu802 and by 16.9%, 13.4% and 14.0% in Nipponbare, compared with roots treated with Al alone, as well as pectin methylesterase (PME) activity.
33120266	3	67	theme	nitrate	544:550	arg1	NR					563:564	NR	563:564	NR	563:564	In this study, we found that the application of 50 µM Al induces nitrate reductase (NR) activity and endogenous NO production, but not nitric oxide synthase (NOS) activity in two rice genotypes.
33120266	3	67	theme	nitrate	544:550	arg1	reductase					552:560	nitrate reductase	544:560	nitrate reductase (NR) activity	544:574	In this study, we found that the application of 50 µM Al induces nitrate reductase (NR) activity and endogenous NO production, but not nitric oxide synthase (NOS) activity in two rice genotypes.
33120266	5	68	from	%	1079:1079	arg1	Nipponbare					1084:1093	Nipponbare	1084:1093	Nipponbare	1084:1093	The addition of SNP significantly decreased Al-induced accumulation of pectin, hemicellulose 1 and hemicellulose 2 by 43.1%, 13.1% and 19.2% in Zhefu802 and by 16.9%, 13.4% and 14.0% in Nipponbare, compared with roots treated with Al alone, as well as pectin methylesterase (PME) activity.
33120266	6	69	theme	Al-induced	1275:1284	arg1	damage					1296:1301	the Al-induced structure damage	1271:1301	the Al-induced structure damage to cell walls	1271:1315	Therefore, the content of Al absorbed in cell walls was decreased, indicating that the Al-induced structure damage to cell walls was alleviated.
33120266	6	70	theme	structure	1286:1294	arg1	damage					1296:1301	the Al-induced structure damage	1271:1301	the Al-induced structure damage to cell walls	1271:1315	Therefore, the content of Al absorbed in cell walls was decreased, indicating that the Al-induced structure damage to cell walls was alleviated.
33120266	7	71	theme	lipid	1490:1494	arg1	peroxidation					1496:1507	lipid peroxidation	1490:1507	lipid peroxidation	1490:1507	Furthermore, the activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) treated by Al were all increased by SNP pretreatment, and the lipid peroxidation and damage to plasma membrane of root tips detected with Schiff's reagent and Evans blue reduced.
33120266	2	72	theme	important	279:287	arg1	role					289:292	an important role	276:292	an important role	276:292	Nitric oxide (NO) plays an important role in plant growth and responses to abiotic stresses, however, it is not clear whether NO is connected with the response of cell wall to aluminum (Al) tolerance in rice (Oryza sativa L.).
33120266	9	73	theme	lipid	1794:1798	arg1	peroxidation					1800:1811	lipid peroxidation	1794:1811	lipid peroxidation	1794:1811	These results indicated that by regulating the Al-binding capacity to cell walls and lipid peroxidation, the structure of cell walls can be stabilized and that Al toxicity in rice can be alleviated with increased NO.
33120266	2	74	from	tolerance	442:450	arg1	rice					455:458	rice	455:458	rice (Oryza sativa L.)	455:476	Nitric oxide (NO) plays an important role in plant growth and responses to abiotic stresses, however, it is not clear whether NO is connected with the response of cell wall to aluminum (Al) tolerance in rice (Oryza sativa L.).
33120266	1	75	theme	toxic	196:200	arg1	ions					202:205	toxic ions	196:205	toxic ions entering into protoplast	196:230	Plant cell wall, the first interface or barrier for toxic ions entering into protoplast, suffers from risk.
33120266	8	76	theme	NR	1676:1677	arg1	NaN3					1690:1693	NaN3	1690:1693	NaN3	1690:1693	In contrast, the effect was abolished when NO scavenger (cPTIO), and NR inhibitor (NaN3), were added.
33120266	8	76	theme	NR	1676:1677	arg1	inhibitor					1679:1687	NR inhibitor	1676:1687	NR inhibitor (NaN3)	1676:1694	In contrast, the effect was abolished when NO scavenger (cPTIO), and NR inhibitor (NaN3), were added.
33120266	2	77	dep	Oryza	461:465	arg1	sativa					467:472	Oryza sativa L	461:474	Oryza sativa L.	461:475	Nitric oxide (NO) plays an important role in plant growth and responses to abiotic stresses, however, it is not clear whether NO is connected with the response of cell wall to aluminum (Al) tolerance in rice (Oryza sativa L.).
33120266	2	77	dep	Oryza	461:465	arg1	L					474:474	L	474:474	Oryza sativa L.	461:475	Nitric oxide (NO) plays an important role in plant growth and responses to abiotic stresses, however, it is not clear whether NO is connected with the response of cell wall to aluminum (Al) tolerance in rice (Oryza sativa L.).
33120266	7	78	theme	SNP	1464:1466	arg1	pretreatment					1468:1479	SNP pretreatment	1464:1479	SNP pretreatment	1464:1479	Furthermore, the activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) treated by Al were all increased by SNP pretreatment, and the lipid peroxidation and damage to plasma membrane of root tips detected with Schiff's reagent and Evans blue reduced.
33120266	1	79	theme	Plant	144:148	arg1	barrier					184:190	barrier	184:190	barrier	184:190	Plant cell wall, the first interface or barrier for toxic ions entering into protoplast, suffers from risk.
33120266	1	79	theme	Plant	144:148	arg1	interface					171:179	the first interface	161:179	the first interface	161:179	Plant cell wall, the first interface or barrier for toxic ions entering into protoplast, suffers from risk.
33120266	1	79	theme	Plant	144:148	arg1	wall					155:158	Plant cell wall	144:158	Plant cell wall	144:158	Plant cell wall, the first interface or barrier for toxic ions entering into protoplast, suffers from risk.
33120266	9	80	theme	cell	1831:1834	arg1	walls					1836:1840	cell walls	1831:1840	cell walls	1831:1840	These results indicated that by regulating the Al-binding capacity to cell walls and lipid peroxidation, the structure of cell walls can be stabilized and that Al toxicity in rice can be alleviated with increased NO.
34894552	0	0	theme	symmetrical	95:105	arg1	supercapacitor					107:120	symmetrical supercapacitor	95:120	symmetrical supercapacitor	95:120	Intertwined carbon networks derived from Polyimide/Cellulose composite as porous electrode for symmetrical supercapacitor.
34894552	4	1	theme	power	928:932	arg1	density					934:940	power density	928:940	power density of 800 W kg-1	928:954	More impressively, the CPC-5 based symmetrical supercapacitor affords a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1, as well as an exceptional capacitance retention of 91.4% after 10,000 cycles.
34894552	2	2	theme	"	393:393	arg1	carbonization					395:407	a facile "one-step" carbonization	375:407	a facile "one-step" carbonization	375:407	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
34894552	5	3	theme	effective	1056:1064	arg1	strategy					1066:1073	an effective strategy	1053:1073	an effective strategy to yield a promising polyimide derived carbon material for high-performance supercapacitors	1053:1165	This work affords an effective strategy to yield a promising polyimide derived carbon material for high-performance supercapacitors.
34894552	3	4	theme	optimized	708:716	arg1	electrode					724:732	the optimized CPC-5 electrode	704:732	the optimized CPC-5 electrode	704:732	Benefitting from the high specific surface area (951 m2 g-1) and uniformly distributed pores, the optimized CPC-5 electrode exhibits an outstanding specific capacitance of 300F g-1 in 6.0 M KOH electrolyte.
34894552	2	5	theme	one-step	385:392	arg1	carbonization					395:407	a facile "one-step" carbonization	375:407	a facile "one-step" carbonization	375:407	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
34894552	2	6	theme	porous	561:566	arg1	networks					568:575	intertwined porous networks	549:575	intertwined porous networks	549:575	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
34894552	3	7	theme	CPC-5	718:722	arg1	electrode					724:732	the optimized CPC-5 electrode	704:732	the optimized CPC-5 electrode	704:732	Benefitting from the high specific surface area (951 m2 g-1) and uniformly distributed pores, the optimized CPC-5 electrode exhibits an outstanding specific capacitance of 300F g-1 in 6.0 M KOH electrolyte.
34894552	4	8	theme	Wh	917:918	arg1	kg-1					920:923	22.6 Wh kg-1	912:923	22.6 Wh kg-1	912:923	More impressively, the CPC-5 based symmetrical supercapacitor affords a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1, as well as an exceptional capacitance retention of 91.4% after 10,000 cycles.
34894552	2	9	theme	CPC	342:344	arg1	composite					347:355	three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite	287:355	three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite	287:355	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
34894552	2	10	theme	intertwined	549:559	arg1	networks					568:575	intertwined porous networks	549:575	intertwined porous networks	549:575	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
34894552	3	11	theme	specific	758:765	arg1	capacitance					767:777	an outstanding specific capacitance	743:777	an outstanding specific capacitance of 300F g-1	743:789	Benefitting from the high specific surface area (951 m2 g-1) and uniformly distributed pores, the optimized CPC-5 electrode exhibits an outstanding specific capacitance of 300F g-1 in 6.0 M KOH electrolyte.
34894552	4	12	theme	%	1012:1012	arg1	retention					995:1003	an exceptional capacitance retention	968:1003	an exceptional capacitance retention of 91.4% after 10,000 cycles	968:1032	More impressively, the CPC-5 based symmetrical supercapacitor affords a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1, as well as an exceptional capacitance retention of 91.4% after 10,000 cycles.
34894552	4	12	theme	%	1012:1012	arg1	density					901:907	a high energy density	887:907	a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1	887:954	More impressively, the CPC-5 based symmetrical supercapacitor affords a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1, as well as an exceptional capacitance retention of 91.4% after 10,000 cycles.
34894552	4	13	theme	exceptional	971:981	arg1	retention					995:1003	an exceptional capacitance retention	968:1003	an exceptional capacitance retention of 91.4% after 10,000 cycles	968:1032	More impressively, the CPC-5 based symmetrical supercapacitor affords a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1, as well as an exceptional capacitance retention of 91.4% after 10,000 cycles.
34894552	5	14	theme	carbon	1114:1119	arg1	material					1121:1128	carbon material	1114:1128	carbon material for high-performance supercapacitors	1114:1165	This work affords an effective strategy to yield a promising polyimide derived carbon material for high-performance supercapacitors.
34894552	0	15	attach	derived	28:34	arg1	Polyimide/Cellulose					41:59	Polyimide/Cellulose	41:59	Polyimide/Cellulose	41:59	Intertwined carbon networks derived from Polyimide/Cellulose composite as porous electrode for symmetrical supercapacitor.
34894552	0	15	attach	derived	28:34	arg2	networks					19:26	Intertwined carbon networks	0:26	Intertwined carbon networks derived from Polyimide/Cellulose	0:59	Intertwined carbon networks derived from Polyimide/Cellulose composite as porous electrode for symmetrical supercapacitor.
34894552	2	16	theme	polyimide/cellulose	321:339	arg1	composite					347:355	three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite	287:355	three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite	287:355	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
34894552	1	17	theme	electrochemical	197:211	arg1	performance					213:223	the electrochemical performance	193:223	the electrochemical performance of carbon materials for supercapacitor	193:262	Designing intertwined porous structure is highly desirable to improve the electrochemical performance of carbon materials for supercapacitor.
34894552	2	18	theme	carbonized	310:319	arg1	composite					347:355	three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite	287:355	three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite	287:355	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
34894552	2	19	dep	three-dimensional	287:303	arg1	3D					306:307	3D	306:307	3D	306:307	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
34894552	0	20	theme	carbon	12:17	arg1	networks					19:26	Intertwined carbon networks	0:26	Intertwined carbon networks derived from Polyimide/Cellulose	0:59	Intertwined carbon networks derived from Polyimide/Cellulose composite as porous electrode for symmetrical supercapacitor.
34894552	3	21	theme	outstanding	746:756	arg1	capacitance					767:777	an outstanding specific capacitance	743:777	an outstanding specific capacitance of 300F g-1	743:789	Benefitting from the high specific surface area (951 m2 g-1) and uniformly distributed pores, the optimized CPC-5 electrode exhibits an outstanding specific capacitance of 300F g-1 in 6.0 M KOH electrolyte.
34894552	4	22	theme	energy	894:899	arg1	density					901:907	a high energy density	887:907	a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1	887:954	More impressively, the CPC-5 based symmetrical supercapacitor affords a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1, as well as an exceptional capacitance retention of 91.4% after 10,000 cycles.
34894552	0	23	theme	Intertwined	0:10	arg1	networks					19:26	Intertwined carbon networks	0:26	Intertwined carbon networks derived from Polyimide/Cellulose	0:59	Intertwined carbon networks derived from Polyimide/Cellulose composite as porous electrode for symmetrical supercapacitor.
34894552	3	24	theme	g-1	787:789	arg1	capacitance					767:777	an outstanding specific capacitance	743:777	an outstanding specific capacitance of 300F g-1	743:789	Benefitting from the high specific surface area (951 m2 g-1) and uniformly distributed pores, the optimized CPC-5 electrode exhibits an outstanding specific capacitance of 300F g-1 in 6.0 M KOH electrolyte.
34894552	4	25	theme	high	889:892	arg1	density					901:907	a high energy density	887:907	a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1	887:954	More impressively, the CPC-5 based symmetrical supercapacitor affords a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1, as well as an exceptional capacitance retention of 91.4% after 10,000 cycles.
34894552	1	26	theme	carbon	228:233	arg1	materials					235:243	carbon materials	228:243	carbon materials	228:243	Designing intertwined porous structure is highly desirable to improve the electrochemical performance of carbon materials for supercapacitor.
34894552	2	27	theme	obtained	584:591	arg1	skeleton					600:607	the obtained carbon skeleton	580:607	the obtained carbon skeleton	580:607	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
34894552	2	28	from	carbonization	395:407	arg1	capable					454:460	capable	454:460	capable	454:460	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
34894552	1	29	theme	Designing	123:131	arg1	structure					152:160	Designing intertwined porous structure	123:160	Designing intertwined porous structure	123:160	Designing intertwined porous structure is highly desirable to improve the electrochemical performance of carbon materials for supercapacitor.
34894552	4	30	theme	based	846:850	arg1	supercapacitor					864:877	the CPC-5 based symmetrical supercapacitor	836:877	the CPC-5 based symmetrical supercapacitor	836:877	More impressively, the CPC-5 based symmetrical supercapacitor affords a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1, as well as an exceptional capacitance retention of 91.4% after 10,000 cycles.
34894552	1	31	theme	materials	235:243	arg1	performance					213:223	the electrochemical performance	193:223	the electrochemical performance of carbon materials for supercapacitor	193:262	Designing intertwined porous structure is highly desirable to improve the electrochemical performance of carbon materials for supercapacitor.
34894552	1	32	theme	intertwined	133:143	arg1	structure					152:160	Designing intertwined porous structure	123:160	Designing intertwined porous structure	123:160	Designing intertwined porous structure is highly desirable to improve the electrochemical performance of carbon materials for supercapacitor.
34894552	2	33	theme	acid	512:515	arg1	structure					490:498	the molecular structure	476:498	the molecular structure of polyamic acid	476:515	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
34894552	3	34	theme	300F	782:785	arg1	g-1					787:789	300F g-1	782:789	300F g-1	782:789	Benefitting from the high specific surface area (951 m2 g-1) and uniformly distributed pores, the optimized CPC-5 electrode exhibits an outstanding specific capacitance of 300F g-1 in 6.0 M KOH electrolyte.
34894552	3	35	theme	high	631:634	arg1	area					653:656	the high specific surface area	627:656	the high specific surface area (951 m2 g-1)	627:669	Benefitting from the high specific surface area (951 m2 g-1) and uniformly distributed pores, the optimized CPC-5 electrode exhibits an outstanding specific capacitance of 300F g-1 in 6.0 M KOH electrolyte.
34894552	3	35	theme	high	631:634	arg1	g-1					666:668	951 m2 g-1	659:668	951 m2 g-1	659:668	Benefitting from the high specific surface area (951 m2 g-1) and uniformly distributed pores, the optimized CPC-5 electrode exhibits an outstanding specific capacitance of 300F g-1 in 6.0 M KOH electrolyte.
34894552	2	36	theme	polyamic	503:510	arg1	acid					512:515	polyamic acid	503:515	polyamic acid	503:515	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
34894552	2	37	theme	networks	568:575	arg1	formation					536:544	the formation	532:544	the formation of intertwined porous networks in the obtained carbon skeleton	532:607	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
34894552	5	38	theme	promising	1086:1094	arg1	polyimide					1096:1104	a promising polyimide	1084:1104	a promising polyimide derived carbon material for high-performance supercapacitors	1084:1165	This work affords an effective strategy to yield a promising polyimide derived carbon material for high-performance supercapacitors.
34894552	3	39	theme	specific	636:643	arg1	area					653:656	the high specific surface area	627:656	the high specific surface area (951 m2 g-1)	627:669	Benefitting from the high specific surface area (951 m2 g-1) and uniformly distributed pores, the optimized CPC-5 electrode exhibits an outstanding specific capacitance of 300F g-1 in 6.0 M KOH electrolyte.
34894552	3	39	theme	specific	636:643	arg1	g-1					666:668	951 m2 g-1	659:668	951 m2 g-1	659:668	Benefitting from the high specific surface area (951 m2 g-1) and uniformly distributed pores, the optimized CPC-5 electrode exhibits an outstanding specific capacitance of 300F g-1 in 6.0 M KOH electrolyte.
34894552	2	40	theme	cross-linked	432:443	arg1	agent					445:449	cross-linked agent	432:449	cross-linked agent	432:449	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
34894552	4	41	theme	symmetrical	852:862	arg1	supercapacitor					864:877	the CPC-5 based symmetrical supercapacitor	836:877	the CPC-5 based symmetrical supercapacitor	836:877	More impressively, the CPC-5 based symmetrical supercapacitor affords a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1, as well as an exceptional capacitance retention of 91.4% after 10,000 cycles.
34894552	4	42	theme	capacitance	983:993	arg1	retention					995:1003	an exceptional capacitance retention	968:1003	an exceptional capacitance retention of 91.4% after 10,000 cycles	968:1032	More impressively, the CPC-5 based symmetrical supercapacitor affords a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1, as well as an exceptional capacitance retention of 91.4% after 10,000 cycles.
34894552	1	43	theme	porous	145:150	arg1	structure					152:160	Designing intertwined porous structure	123:160	Designing intertwined porous structure	123:160	Designing intertwined porous structure is highly desirable to improve the electrochemical performance of carbon materials for supercapacitor.
34894552	4	44	theme	kg-1	920:923	arg1	retention					995:1003	an exceptional capacitance retention	968:1003	an exceptional capacitance retention of 91.4% after 10,000 cycles	968:1032	More impressively, the CPC-5 based symmetrical supercapacitor affords a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1, as well as an exceptional capacitance retention of 91.4% after 10,000 cycles.
34894552	4	44	theme	kg-1	920:923	arg1	density					901:907	a high energy density	887:907	a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1	887:954	More impressively, the CPC-5 based symmetrical supercapacitor affords a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1, as well as an exceptional capacitance retention of 91.4% after 10,000 cycles.
34894552	4	45	from	density	934:940	arg1	retention					995:1003	an exceptional capacitance retention	968:1003	an exceptional capacitance retention of 91.4% after 10,000 cycles	968:1032	More impressively, the CPC-5 based symmetrical supercapacitor affords a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1, as well as an exceptional capacitance retention of 91.4% after 10,000 cycles.
34894552	4	45	from	density	934:940	arg1	density					901:907	a high energy density	887:907	a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1	887:954	More impressively, the CPC-5 based symmetrical supercapacitor affords a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1, as well as an exceptional capacitance retention of 91.4% after 10,000 cycles.
34894552	2	46	from	formation	536:544	arg1	skeleton					600:607	the obtained carbon skeleton	580:607	the obtained carbon skeleton	580:607	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
34894552	2	47	theme	facile	377:382	arg1	carbonization					395:407	a facile "one-step" carbonization	375:407	a facile "one-step" carbonization	375:407	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
34894552	3	48	theme	KOH	800:802	arg1	electrolyte					804:814	KOH electrolyte	800:814	KOH electrolyte	800:814	Benefitting from the high specific surface area (951 m2 g-1) and uniformly distributed pores, the optimized CPC-5 electrode exhibits an outstanding specific capacitance of 300F g-1 in 6.0 M KOH electrolyte.
34894552	2	49	link	cross-linked	432:443	arg1	agent					445:449	cross-linked agent	432:449	cross-linked agent	432:449	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
34894552	4	50	theme	CPC-5	840:844	arg1	supercapacitor					864:877	the CPC-5 based symmetrical supercapacitor	836:877	the CPC-5 based symmetrical supercapacitor	836:877	More impressively, the CPC-5 based symmetrical supercapacitor affords a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1, as well as an exceptional capacitance retention of 91.4% after 10,000 cycles.
34894552	2	51	theme	carbon	593:598	arg1	skeleton					600:607	the obtained carbon skeleton	580:607	the obtained carbon skeleton	580:607	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
34894552	3	52	theme	distributed	685:695	arg1	pores					697:701	uniformly distributed pores	675:701	uniformly distributed pores	675:701	Benefitting from the high specific surface area (951 m2 g-1) and uniformly distributed pores, the optimized CPC-5 electrode exhibits an outstanding specific capacitance of 300F g-1 in 6.0 M KOH electrolyte.
34894552	3	53	theme	951 m2	659:664	arg1	area					653:656	the high specific surface area	627:656	the high specific surface area (951 m2 g-1)	627:669	Benefitting from the high specific surface area (951 m2 g-1) and uniformly distributed pores, the optimized CPC-5 electrode exhibits an outstanding specific capacitance of 300F g-1 in 6.0 M KOH electrolyte.
34894552	3	53	theme	951 m2	659:664	arg1	g-1					666:668	951 m2 g-1	659:668	951 m2 g-1	659:668	Benefitting from the high specific surface area (951 m2 g-1) and uniformly distributed pores, the optimized CPC-5 electrode exhibits an outstanding specific capacitance of 300F g-1 in 6.0 M KOH electrolyte.
34894552	4	54	theme	800 W kg-1	945:954	arg1	density					934:940	power density	928:940	power density of 800 W kg-1	928:954	More impressively, the CPC-5 based symmetrical supercapacitor affords a high energy density of 22.6 Wh kg-1 at power density of 800 W kg-1, as well as an exceptional capacitance retention of 91.4% after 10,000 cycles.
34894552	3	55	theme	surface	645:651	arg1	area					653:656	the high specific surface area	627:656	the high specific surface area (951 m2 g-1)	627:669	Benefitting from the high specific surface area (951 m2 g-1) and uniformly distributed pores, the optimized CPC-5 electrode exhibits an outstanding specific capacitance of 300F g-1 in 6.0 M KOH electrolyte.
34894552	3	55	theme	surface	645:651	arg1	g-1					666:668	951 m2 g-1	659:668	951 m2 g-1	659:668	Benefitting from the high specific surface area (951 m2 g-1) and uniformly distributed pores, the optimized CPC-5 electrode exhibits an outstanding specific capacitance of 300F g-1 in 6.0 M KOH electrolyte.
34894552	0	56	theme	porous	74:79	arg1	electrode					81:89	porous electrode	74:89	porous electrode for symmetrical supercapacitor	74:120	Intertwined carbon networks derived from Polyimide/Cellulose composite as porous electrode for symmetrical supercapacitor.
34894552	5	57	theme	high-performance	1134:1149	arg1	supercapacitors					1151:1165	high-performance supercapacitors	1134:1165	high-performance supercapacitors	1134:1165	This work affords an effective strategy to yield a promising polyimide derived carbon material for high-performance supercapacitors.
34894552	2	58	theme	three-dimensional	287:303	arg1	composite					347:355	three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite	287:355	three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite	287:355	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
34894552	2	59	from	capable	454:460	arg1	carbonization					395:407	a facile "one-step" carbonization	375:407	a facile "one-step" carbonization	375:407	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
34894552	2	60	theme	molecular	480:488	arg1	structure					490:498	the molecular structure	476:498	the molecular structure of polyamic acid	476:515	In this contribution, three-dimensional (3D) carbonized polyimide/cellulose (CPC) composite is fabricated via a facile "one-step" carbonization, in which cellulose as cross-linked agent is capable of modulating the molecular structure of polyamic acid, thus ensuring the formation of intertwined porous networks in the obtained carbon skeleton.
32643739	7	0	theme	materials	1844:1852	arg1	synthesis					1812:1820	facile effective synthesis	1795:1820	facile effective synthesis	1795:1820	The good electrochemical performance and facile effective synthesis of bio-derived carbon materials with tunable porous structures indicate promising applications in supercapacitors.
32643739	7	0	theme	materials	1844:1852	arg1	performance					1779:1789	good electrochemical performance	1758:1789	good electrochemical performance	1758:1789	The good electrochemical performance and facile effective synthesis of bio-derived carbon materials with tunable porous structures indicate promising applications in supercapacitors.
32643739	5	1	from	performance	1467:1477	arg1	capacitance					1413:1423	a high specific capacitance	1397:1423	a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability	1397:1548	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	5	1	from	performance	1467:1477	arg1	g-1					1434:1436	420 F g-1	1428:1436	420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability	1428:1548	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	7	2	dep	performance	1779:1789	arg1	The					1754:1756	The	1754:1756	The	1754:1756	The good electrochemical performance and facile effective synthesis of bio-derived carbon materials with tunable porous structures indicate promising applications in supercapacitors.
32643739	5	3	theme	A	1630:1630	arg1	g-1					1632:1634	5 A g-1	1628:1634	5 A g-1	1628:1634	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	2	4	theme	subsequent	614:623	arg1	carbonization/activation					625:648	subsequent carbonization/activation	614:648	subsequent carbonization/activation	614:648	Here, hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite have been successfully prepared by thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation.
32643739	7	5	theme	bio-derived	1825:1835	arg1	materials					1844:1852	bio-derived carbon materials	1825:1852	bio-derived carbon materials with tunable porous structures	1825:1883	The good electrochemical performance and facile effective synthesis of bio-derived carbon materials with tunable porous structures indicate promising applications in supercapacitors.
32643739	5	6	theme	75	1484:1485	arg1	%					1486:1486	%	1486:1486	%	1486:1486	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	7	7	link	bio-derived	1825:1835	arg1	materials					1844:1852	bio-derived carbon materials	1825:1852	bio-derived carbon materials with tunable porous structures	1825:1883	The good electrochemical performance and facile effective synthesis of bio-derived carbon materials with tunable porous structures indicate promising applications in supercapacitors.
32643739	4	8	theme	%	1191:1191	arg1	SSA					1129:1131	a high SSA	1122:1131	a high SSA of 2161 m2 g-1	1122:1146	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	4	8	theme	%	1191:1191	arg1	micropores					1062:1071	moderate micropores	1053:1071	moderate micropores	1053:1071	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	4	8	theme	%	1191:1191	arg1	content					1176:1182	a maximum oxygen-dopant content	1152:1182	a maximum oxygen-dopant content of 9.99%	1152:1191	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	4	8	theme	%	1191:1191	arg1	macropores					1110:1119	interconnected macropores	1095:1119	interconnected macropores	1095:1119	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	4	8	theme	%	1191:1191	arg1	mesopores					1084:1092	favorable mesopores	1074:1092	favorable mesopores	1074:1092	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	5	9	theme	charge-discharge	1601:1616	arg1	cycles					1618:1623	10 000 continuous charge-discharge cycles	1583:1623	10 000 continuous charge-discharge cycles at 5 A g-1	1583:1634	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	1	10	theme	anticipated	203:213	arg1	microstructures					215:229	anticipated microstructures	203:229	anticipated microstructures	203:229	The controlled design and synthesis of porous carbons with anticipated microstructures and morphologies, and a high specific surface area (SSA) have been focused on for supercapacitor development.
32643739	7	11	theme	facile	1795:1800	arg1	synthesis					1812:1820	facile effective synthesis	1795:1820	facile effective synthesis	1795:1820	The good electrochemical performance and facile effective synthesis of bio-derived carbon materials with tunable porous structures indicate promising applications in supercapacitors.
32643739	5	12	theme	excellent	1452:1460	arg1	performance					1467:1477	excellent rate performance	1452:1477	excellent rate performance with 75% capacitance retention at 20 A g-1	1452:1520	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	4	13	theme	carbon	990:995	arg1	material					997:1004	The obtained porous carbon material	970:1004	The obtained porous carbon material	970:1004	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	4	14	theme	oxygen-dopant	1162:1174	arg1	content					1176:1182	a maximum oxygen-dopant content	1152:1182	a maximum oxygen-dopant content of 9.99%	1152:1191	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	6	15	theme	energy	1723:1728	arg1	density					1730:1736	a moderate energy density	1712:1736	a moderate energy density of 20 W h kg-1	1712:1751	Additionally, the assembled supercapacitor based on porous carbon displays a moderate energy density of 20 W h kg-1.
32643739	2	16	theme	cellulose	473:481	arg1	composite					488:496	a natural-based bacterial cellulose (BC) composite	447:496	a natural-based bacterial cellulose (BC) composite	447:496	Here, hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite have been successfully prepared by thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation.
32643739	3	17	theme	activation	813:822	arg1	temperature					824:834	activation temperature	813:834	activation temperature	813:834	The SSA and porous architectures can be controlled by fine-tuning the preparation conditions such as the precursor morphology and structure, activator dosage and activation temperature, and the relationships between the super-capacitive properties and the SSA and pore size distribution have been further investigated.
32643739	1	18	theme	controlled	148:157	arg1	design					159:164	controlled design	148:164	controlled design	148:164	The controlled design and synthesis of porous carbons with anticipated microstructures and morphologies, and a high specific surface area (SSA) have been focused on for supercapacitor development.
32643739	3	19	theme	pore	915:918	arg1	distribution					925:936	the SSA and pore size distribution	903:936	distribution	925:936	The SSA and porous architectures can be controlled by fine-tuning the preparation conditions such as the precursor morphology and structure, activator dosage and activation temperature, and the relationships between the super-capacitive properties and the SSA and pore size distribution have been further investigated.
32643739	5	20	from	g-1	1518:1520	arg1	stability					1540:1548	good cycling stability	1527:1548	good cycling stability	1527:1548	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	5	20	from	g-1	1518:1520	arg1	g-1					1447:1449	0.5 A g-1	1441:1449	0.5 A g-1	1441:1449	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	5	20	from	g-1	1518:1520	arg1	performance					1467:1477	excellent rate performance	1452:1477	excellent rate performance with 75% capacitance retention at 20 A g-1	1452:1520	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	4	21	theme	obtained	974:981	arg1	material					997:1004	The obtained porous carbon material	970:1004	The obtained porous carbon material	970:1004	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	5	22	theme	porous	1342:1347	arg1	electrode					1356:1364	the obtained porous carbon electrode	1329:1364	the obtained porous carbon electrode used in a supercapacitor	1329:1389	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	3	23	theme	porous	663:668	arg1	architectures					670:682	The SSA and porous architectures	651:682	architectures	670:682	The SSA and porous architectures can be controlled by fine-tuning the preparation conditions such as the precursor morphology and structure, activator dosage and activation temperature, and the relationships between the super-capacitive properties and the SSA and pore size distribution have been further investigated.
32643739	4	24	theme	moderate	1053:1060	arg1	micropores					1062:1071	moderate micropores	1053:1071	moderate micropores	1053:1071	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	0	25	from	monoliths	113:121	arg1	preparation					11:21	preparation	11:21	preparation of interconnected 3D hierarchical porous carbons from bacterial cellulose-based composite monoliths for supercapacitors	11:141	Controlled preparation of interconnected 3D hierarchical porous carbons from bacterial cellulose-based composite monoliths for supercapacitors.
32643739	0	25	from	monoliths	113:121	arg1	carbons					64:70	interconnected 3D hierarchical porous carbons	26:70	interconnected 3D hierarchical porous carbons from bacterial cellulose-based composite monoliths for supercapacitors	26:141	Controlled preparation of interconnected 3D hierarchical porous carbons from bacterial cellulose-based composite monoliths for supercapacitors.
32643739	7	26	theme	good	1758:1761	arg1	performance					1779:1789	good electrochemical performance	1758:1789	good electrochemical performance	1758:1789	The good electrochemical performance and facile effective synthesis of bio-derived carbon materials with tunable porous structures indicate promising applications in supercapacitors.
32643739	3	27	theme	SSA	655:657	arg1	architectures					670:682	The SSA and porous architectures	651:682	architectures	670:682	The SSA and porous architectures can be controlled by fine-tuning the preparation conditions such as the precursor morphology and structure, activator dosage and activation temperature, and the relationships between the super-capacitive properties and the SSA and pore size distribution have been further investigated.
32643739	7	28	from	applications	1904:1915	arg1	supercapacitors					1920:1934	supercapacitors	1920:1934	supercapacitors	1920:1934	The good electrochemical performance and facile effective synthesis of bio-derived carbon materials with tunable porous structures indicate promising applications in supercapacitors.
32643739	5	29	theme	A	1516:1516	arg1	g-1					1518:1520	20 A g-1	1513:1520	20 A g-1	1513:1520	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	5	30	theme	features	1319:1326	arg1	effects					1302:1308	the synergistic effects	1286:1308	the synergistic effects of these features	1286:1326	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	0	31	theme	cellulose-based	87:101	arg1	monoliths					113:121	bacterial cellulose-based composite monoliths	77:121	bacterial cellulose-based composite monoliths for supercapacitors	77:141	Controlled preparation of interconnected 3D hierarchical porous carbons from bacterial cellulose-based composite monoliths for supercapacitors.
32643739	1	32	with	carbons	190:196	arg1	microstructures					215:229	anticipated microstructures	203:229	anticipated microstructures	203:229	The controlled design and synthesis of porous carbons with anticipated microstructures and morphologies, and a high specific surface area (SSA) have been focused on for supercapacitor development.
32643739	1	32	with	carbons	190:196	arg1	morphologies					235:246	morphologies	235:246	morphologies	235:246	The controlled design and synthesis of porous carbons with anticipated microstructures and morphologies, and a high specific surface area (SSA) have been focused on for supercapacitor development.
32643739	1	33	theme	carbons	190:196	arg1	design					159:164	controlled design	148:164	controlled design	148:164	The controlled design and synthesis of porous carbons with anticipated microstructures and morphologies, and a high specific surface area (SSA) have been focused on for supercapacitor development.
32643739	1	33	theme	carbons	190:196	arg1	SSA					283:285	SSA	283:285	SSA	283:285	The controlled design and synthesis of porous carbons with anticipated microstructures and morphologies, and a high specific surface area (SSA) have been focused on for supercapacitor development.
32643739	1	33	theme	carbons	190:196	arg1	area					277:280	a high specific surface area	253:280	a high specific surface area (SSA)	253:286	The controlled design and synthesis of porous carbons with anticipated microstructures and morphologies, and a high specific surface area (SSA) have been focused on for supercapacitor development.
32643739	1	33	theme	carbons	190:196	arg1	synthesis					170:178	synthesis	170:178	synthesis	170:178	The controlled design and synthesis of porous carbons with anticipated microstructures and morphologies, and a high specific surface area (SSA) have been focused on for supercapacitor development.
32643739	4	34	theme	hierarchical	1018:1029	arg1	structure					1038:1046	a hierarchical porous structure	1016:1046	a hierarchical porous structure	1016:1046	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	6	35	theme	kg-1	1748:1751	arg1	density					1730:1736	a moderate energy density	1712:1736	a moderate energy density of 20 W h kg-1	1712:1751	Additionally, the assembled supercapacitor based on porous carbon displays a moderate energy density of 20 W h kg-1.
32643739	1	36	dep	design	159:164	arg1	The					144:146	The	144:146	The	144:146	The controlled design and synthesis of porous carbons with anticipated microstructures and morphologies, and a high specific surface area (SSA) have been focused on for supercapacitor development.
32643739	3	37	theme	super-capacitive	871:886	arg1	properties					888:897	the super-capacitive properties	867:897	the super-capacitive properties	867:897	The SSA and porous architectures can be controlled by fine-tuning the preparation conditions such as the precursor morphology and structure, activator dosage and activation temperature, and the relationships between the super-capacitive properties and the SSA and pore size distribution have been further investigated.
32643739	6	38	theme	assembled	1655:1663	arg1	supercapacitor					1665:1678	the assembled supercapacitor	1651:1678	the assembled supercapacitor based on porous carbon	1651:1701	Additionally, the assembled supercapacitor based on porous carbon displays a moderate energy density of 20 W h kg-1.
32643739	2	39	theme	phase	551:555	arg1	separation					557:566	thermally induced phase separation	533:566	thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation	533:648	Here, hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite have been successfully prepared by thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation.
32643739	6	40	theme	W	1744:1744	arg1	kg-1					1748:1751	20 W h kg-1	1741:1751	20 W h kg-1	1741:1751	Additionally, the assembled supercapacitor based on porous carbon displays a moderate energy density of 20 W h kg-1.
32643739	5	41	theme	capacitance	1488:1498	arg1	retention					1500:1508	75% capacitance retention	1484:1508	75% capacitance retention	1484:1508	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	2	42	theme	BC	484:485	arg1	composite					488:496	a natural-based bacterial cellulose (BC) composite	447:496	a natural-based bacterial cellulose (BC) composite	447:496	Here, hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite have been successfully prepared by thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation.
32643739	5	43	theme	specific	1404:1411	arg1	capacitance					1413:1423	a high specific capacitance	1397:1423	a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability	1397:1548	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	2	44	theme	interconnected	390:403	arg1	morphology					423:432	an interconnected three-dimensional morphology	387:432	an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite	387:496	Here, hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite have been successfully prepared by thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation.
32643739	4	45	theme	high	1124:1127	arg1	SSA					1129:1131	a high SSA	1122:1131	a high SSA of 2161 m2 g-1	1122:1146	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	5	46	theme	∼96.1	1555:1559	arg1	%					1560:1560	%	1560:1560	%	1560:1560	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	2	47	theme	natural-based	449:461	arg1	composite					488:496	a natural-based bacterial cellulose (BC) composite	447:496	a natural-based bacterial cellulose (BC) composite	447:496	Here, hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite have been successfully prepared by thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation.
32643739	5	48	with	g-1	1447:1449	arg1	retention					1500:1508	75% capacitance retention	1484:1508	75% capacitance retention	1484:1508	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	0	49	theme	3D	41:42	arg1	carbons					64:70	interconnected 3D hierarchical porous carbons	26:70	interconnected 3D hierarchical porous carbons from bacterial cellulose-based composite monoliths for supercapacitors	26:141	Controlled preparation of interconnected 3D hierarchical porous carbons from bacterial cellulose-based composite monoliths for supercapacitors.
32643739	1	50	theme	supercapacitor	313:326	arg1	development					328:338	supercapacitor development	313:338	supercapacitor development	313:338	The controlled design and synthesis of porous carbons with anticipated microstructures and morphologies, and a high specific surface area (SSA) have been focused on for supercapacitor development.
32643739	2	51	attach	derived	434:440	arg1	composite					488:496	a natural-based bacterial cellulose (BC) composite	447:496	a natural-based bacterial cellulose (BC) composite	447:496	Here, hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite have been successfully prepared by thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation.
32643739	2	51	attach	derived	434:440	arg2	morphology					423:432	an interconnected three-dimensional morphology	387:432	an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite	387:496	Here, hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite have been successfully prepared by thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation.
32643739	4	52	theme	interconnected	1095:1108	arg1	macropores					1110:1119	interconnected macropores	1095:1119	interconnected macropores	1095:1119	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	3	53	dep	precursor	756:764	arg1	morphology					766:775	morphology	766:775	morphology	766:775	The SSA and porous architectures can be controlled by fine-tuning the preparation conditions such as the precursor morphology and structure, activator dosage and activation temperature, and the relationships between the super-capacitive properties and the SSA and pore size distribution have been further investigated.
32643739	5	54	theme	good	1527:1530	arg1	stability					1540:1548	good cycling stability	1527:1548	good cycling stability	1527:1548	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	0	55	theme	porous	57:62	arg1	carbons					64:70	interconnected 3D hierarchical porous carbons	26:70	interconnected 3D hierarchical porous carbons from bacterial cellulose-based composite monoliths for supercapacitors	26:141	Controlled preparation of interconnected 3D hierarchical porous carbons from bacterial cellulose-based composite monoliths for supercapacitors.
32643739	5	56	with	performance	1467:1477	arg1	retention					1500:1508	75% capacitance retention	1484:1508	75% capacitance retention	1484:1508	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	7	57	theme	porous	1867:1872	arg1	structures					1874:1883	tunable porous structures	1859:1883	tunable porous structures	1859:1883	The good electrochemical performance and facile effective synthesis of bio-derived carbon materials with tunable porous structures indicate promising applications in supercapacitors.
32643739	2	58	with	carbons	367:373	arg1	morphology					423:432	an interconnected three-dimensional morphology	387:432	an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite	387:496	Here, hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite have been successfully prepared by thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation.
32643739	3	59	theme	preparation	721:731	arg1	dosage					802:807	activator dosage	792:807	activator dosage	792:807	The SSA and porous architectures can be controlled by fine-tuning the preparation conditions such as the precursor morphology and structure, activator dosage and activation temperature, and the relationships between the super-capacitive properties and the SSA and pore size distribution have been further investigated.
32643739	3	59	theme	preparation	721:731	arg1	temperature					824:834	activation temperature	813:834	activation temperature	813:834	The SSA and porous architectures can be controlled by fine-tuning the preparation conditions such as the precursor morphology and structure, activator dosage and activation temperature, and the relationships between the super-capacitive properties and the SSA and pore size distribution have been further investigated.
32643739	3	59	theme	preparation	721:731	arg1	conditions					733:742	the preparation conditions	717:742	the preparation conditions such as the precursor morphology and structure, activator dosage and activation temperature	717:834	The SSA and porous architectures can be controlled by fine-tuning the preparation conditions such as the precursor morphology and structure, activator dosage and activation temperature, and the relationships between the super-capacitive properties and the SSA and pore size distribution have been further investigated.
32643739	3	59	theme	preparation	721:731	arg1	precursor					756:764	the precursor morphology and structure	752:789	precursor	756:764	The SSA and porous architectures can be controlled by fine-tuning the preparation conditions such as the precursor morphology and structure, activator dosage and activation temperature, and the relationships between the super-capacitive properties and the SSA and pore size distribution have been further investigated.
32643739	3	59	theme	preparation	721:731	arg1	structure					781:789	structure	781:789	structure	781:789	The SSA and porous architectures can be controlled by fine-tuning the preparation conditions such as the precursor morphology and structure, activator dosage and activation temperature, and the relationships between the super-capacitive properties and the SSA and pore size distribution have been further investigated.
32643739	5	60	theme	A	1445:1445	arg1	g-1					1447:1449	0.5 A g-1	1441:1449	0.5 A g-1	1441:1449	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	2	61	theme	hierarchical	347:358	arg1	HPCs					376:379	HPCs	376:379	HPCs	376:379	Here, hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite have been successfully prepared by thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation.
32643739	2	61	theme	hierarchical	347:358	arg1	carbons					367:373	hierarchical porous carbons	347:373	hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite	347:496	Here, hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite have been successfully prepared by thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation.
32643739	1	62	theme	high	255:258	arg1	SSA					283:285	SSA	283:285	SSA	283:285	The controlled design and synthesis of porous carbons with anticipated microstructures and morphologies, and a high specific surface area (SSA) have been focused on for supercapacitor development.
32643739	1	62	theme	high	255:258	arg1	area					277:280	a high specific surface area	253:280	a high specific surface area (SSA)	253:286	The controlled design and synthesis of porous carbons with anticipated microstructures and morphologies, and a high specific surface area (SSA) have been focused on for supercapacitor development.
32643739	4	63	theme	maximum	1154:1160	arg1	content					1176:1182	a maximum oxygen-dopant content	1152:1182	a maximum oxygen-dopant content of 9.99%	1152:1191	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	7	64	with	materials	1844:1852	arg1	structures					1874:1883	tunable porous structures	1859:1883	tunable porous structures	1859:1883	The good electrochemical performance and facile effective synthesis of bio-derived carbon materials with tunable porous structures indicate promising applications in supercapacitors.
32643739	1	65	theme	surface	269:275	arg1	SSA					283:285	SSA	283:285	SSA	283:285	The controlled design and synthesis of porous carbons with anticipated microstructures and morphologies, and a high specific surface area (SSA) have been focused on for supercapacitor development.
32643739	1	65	theme	surface	269:275	arg1	area					277:280	a high specific surface area	253:280	a high specific surface area (SSA)	253:286	The controlled design and synthesis of porous carbons with anticipated microstructures and morphologies, and a high specific surface area (SSA) have been focused on for supercapacitor development.
32643739	4	66	from	increase	1206:1213	arg1	wettability					1266:1276	wettability	1266:1276	wettability	1266:1276	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	4	66	from	increase	1206:1213	arg1	efficiency					1251:1260	utilization efficiency	1239:1260	utilization efficiency	1239:1260	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	4	66	from	increase	1206:1213	arg1	materials					1229:1237	the active materials utilization efficiency and wettability	1218:1276	the active materials utilization efficiency and wettability	1218:1276	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	7	67	theme	carbon	1837:1842	arg1	materials					1844:1852	bio-derived carbon materials	1825:1852	bio-derived carbon materials with tunable porous structures	1825:1883	The good electrochemical performance and facile effective synthesis of bio-derived carbon materials with tunable porous structures indicate promising applications in supercapacitors.
32643739	5	68	theme	F	1432:1432	arg1	g-1					1434:1436	420 F g-1	1428:1436	420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability	1428:1548	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	5	69	from	g-1	1632:1634	arg1	cycles					1618:1623	10 000 continuous charge-discharge cycles	1583:1623	10 000 continuous charge-discharge cycles at 5 A g-1	1583:1634	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	2	70	theme	carbonization/activation	625:648	arg1	separation					557:566	thermally induced phase separation	533:566	thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation	533:648	Here, hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite have been successfully prepared by thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation.
32643739	4	71	theme	m2	1141:1142	arg1	g-1					1144:1146	2161 m2 g-1	1136:1146	2161 m2 g-1	1136:1146	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	7	72	theme	effective	1802:1810	arg1	synthesis					1812:1820	facile effective synthesis	1795:1820	facile effective synthesis	1795:1820	The good electrochemical performance and facile effective synthesis of bio-derived carbon materials with tunable porous structures indicate promising applications in supercapacitors.
32643739	5	73	theme	continuous	1590:1599	arg1	cycles					1618:1623	10 000 continuous charge-discharge cycles	1583:1623	10 000 continuous charge-discharge cycles at 5 A g-1	1583:1634	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	5	74	theme	%	1486:1486	arg1	retention					1500:1508	75% capacitance retention	1484:1508	75% capacitance retention	1484:1508	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	5	75	with	stability	1540:1548	arg1	retention					1500:1508	75% capacitance retention	1484:1508	75% capacitance retention	1484:1508	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	7	76	theme	electrochemical	1763:1777	arg1	performance					1779:1789	good electrochemical performance	1758:1789	good electrochemical performance	1758:1789	The good electrochemical performance and facile effective synthesis of bio-derived carbon materials with tunable porous structures indicate promising applications in supercapacitors.
32643739	5	77	theme	rate	1462:1465	arg1	performance					1467:1477	excellent rate performance	1452:1477	excellent rate performance with 75% capacitance retention at 20 A g-1	1452:1520	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	4	78	theme	porous	983:988	arg1	material					997:1004	The obtained porous carbon material	970:1004	The obtained porous carbon material	970:1004	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	3	79	theme	activator	792:800	arg1	dosage					802:807	activator dosage	792:807	activator dosage	792:807	The SSA and porous architectures can be controlled by fine-tuning the preparation conditions such as the precursor morphology and structure, activator dosage and activation temperature, and the relationships between the super-capacitive properties and the SSA and pore size distribution have been further investigated.
32643739	4	80	dep	materials	1229:1237	arg1	wettability					1266:1276	wettability	1266:1276	wettability	1266:1276	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	4	80	dep	materials	1229:1237	arg1	efficiency					1251:1260	utilization efficiency	1239:1260	utilization efficiency	1239:1260	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	4	80	dep	materials	1229:1237	arg1	materials					1229:1237	the active materials utilization efficiency and wettability	1218:1276	the active materials utilization efficiency and wettability	1218:1276	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	2	81	theme	poly	571:574	arg1	separation					557:566	thermally induced phase separation	533:566	thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation	533:648	Here, hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite have been successfully prepared by thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation.
32643739	6	82	theme	moderate	1714:1721	arg1	density					1730:1736	a moderate energy density	1712:1736	a moderate energy density of 20 W h kg-1	1712:1751	Additionally, the assembled supercapacitor based on porous carbon displays a moderate energy density of 20 W h kg-1.
32643739	4	83	theme	utilization	1239:1249	arg1	efficiency					1251:1260	utilization efficiency	1239:1260	utilization efficiency	1239:1260	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	4	83	theme	utilization	1239:1249	arg1	materials					1229:1237	the active materials utilization efficiency and wettability	1218:1276	the active materials utilization efficiency and wettability	1218:1276	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	5	84	theme	obtained	1333:1340	arg1	electrode					1356:1364	the obtained porous carbon electrode	1329:1364	the obtained porous carbon electrode used in a supercapacitor	1329:1389	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	3	85	theme	size	920:923	arg1	distribution					925:936	the SSA and pore size distribution	903:936	distribution	925:936	The SSA and porous architectures can be controlled by fine-tuning the preparation conditions such as the precursor morphology and structure, activator dosage and activation temperature, and the relationships between the super-capacitive properties and the SSA and pore size distribution have been further investigated.
32643739	5	86	from	stability	1540:1548	arg1	capacitance					1413:1423	a high specific capacitance	1397:1423	a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability	1397:1548	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	5	86	from	stability	1540:1548	arg1	g-1					1434:1436	420 F g-1	1428:1436	420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability	1428:1548	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	6	87	theme	porous	1689:1694	arg1	carbon					1696:1701	porous carbon	1689:1701	porous carbon	1689:1701	Additionally, the assembled supercapacitor based on porous carbon displays a moderate energy density of 20 W h kg-1.
32643739	5	88	theme	carbon	1349:1354	arg1	electrode					1356:1364	the obtained porous carbon electrode	1329:1364	the obtained porous carbon electrode used in a supercapacitor	1329:1389	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	0	89	theme	bacterial	77:85	arg1	monoliths					113:121	bacterial cellulose-based composite monoliths	77:121	bacterial cellulose-based composite monoliths for supercapacitors	77:141	Controlled preparation of interconnected 3D hierarchical porous carbons from bacterial cellulose-based composite monoliths for supercapacitors.
32643739	4	90	theme	active	1222:1227	arg1	wettability					1266:1276	wettability	1266:1276	wettability	1266:1276	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	4	90	theme	active	1222:1227	arg1	efficiency					1251:1260	utilization efficiency	1239:1260	utilization efficiency	1239:1260	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	4	90	theme	active	1222:1227	arg1	materials					1229:1237	the active materials utilization efficiency and wettability	1218:1276	the active materials utilization efficiency and wettability	1218:1276	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	0	91	theme	composite	103:111	arg1	monoliths					113:121	bacterial cellulose-based composite monoliths	77:121	bacterial cellulose-based composite monoliths for supercapacitors	77:141	Controlled preparation of interconnected 3D hierarchical porous carbons from bacterial cellulose-based composite monoliths for supercapacitors.
32643739	1	92	theme	porous	183:188	arg1	carbons					190:196	porous carbons	183:196	porous carbons with anticipated microstructures and morphologies	183:246	The controlled design and synthesis of porous carbons with anticipated microstructures and morphologies, and a high specific surface area (SSA) have been focused on for supercapacitor development.
32643739	2	93	theme	ethylene-co-vinyl	576:592	arg1	poly					571:574	poly	571:574	poly(ethylene-co-vinyl alcohol) (EVOH)	571:608	Here, hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite have been successfully prepared by thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation.
32643739	2	93	theme	ethylene-co-vinyl	576:592	arg1	alcohol					594:600	ethylene-co-vinyl alcohol	576:600	ethylene-co-vinyl alcohol	576:600	Here, hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite have been successfully prepared by thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation.
32643739	0	94	from	preparation	11:21	arg1	monoliths					113:121	bacterial cellulose-based composite monoliths	77:121	bacterial cellulose-based composite monoliths for supercapacitors	77:141	Controlled preparation of interconnected 3D hierarchical porous carbons from bacterial cellulose-based composite monoliths for supercapacitors.
32643739	6	95	theme	h	1746:1746	arg1	kg-1					1748:1751	20 W h kg-1	1741:1751	20 W h kg-1	1741:1751	Additionally, the assembled supercapacitor based on porous carbon displays a moderate energy density of 20 W h kg-1.
32643739	2	96	theme	induced	543:549	arg1	separation					557:566	thermally induced phase separation	533:566	thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation	533:648	Here, hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite have been successfully prepared by thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation.
32643739	5	97	theme	synergistic	1290:1300	arg1	effects					1302:1308	the synergistic effects	1286:1308	the synergistic effects of these features	1286:1326	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	5	98	theme	high	1399:1402	arg1	capacitance					1413:1423	a high specific capacitance	1397:1423	a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability	1397:1548	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	2	99	theme	three-dimensional	405:421	arg1	morphology					423:432	an interconnected three-dimensional morphology	387:432	an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite	387:496	Here, hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite have been successfully prepared by thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation.
32643739	5	100	from	capacitance	1413:1423	arg1	stability					1540:1548	good cycling stability	1527:1548	good cycling stability	1527:1548	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	5	100	from	capacitance	1413:1423	arg1	g-1					1447:1449	0.5 A g-1	1441:1449	0.5 A g-1	1441:1449	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	5	100	from	capacitance	1413:1423	arg1	performance					1467:1477	excellent rate performance	1452:1477	excellent rate performance with 75% capacitance retention at 20 A g-1	1452:1520	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	0	101	theme	interconnected	26:39	arg1	carbons					64:70	interconnected 3D hierarchical porous carbons	26:70	interconnected 3D hierarchical porous carbons from bacterial cellulose-based composite monoliths for supercapacitors	26:141	Controlled preparation of interconnected 3D hierarchical porous carbons from bacterial cellulose-based composite monoliths for supercapacitors.
32643739	4	102	contain	possesses	1006:1014	arg2	structure					1038:1046	a hierarchical porous structure	1016:1046	a hierarchical porous structure	1016:1046	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	4	102	contain	possesses	1006:1014	arg1	material					997:1004	The obtained porous carbon material	970:1004	The obtained porous carbon material	970:1004	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	2	103	theme	bacterial	463:471	arg1	composite					488:496	a natural-based bacterial cellulose (BC) composite	447:496	a natural-based bacterial cellulose (BC) composite	447:496	Here, hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite have been successfully prepared by thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation.
32643739	5	104	theme	%	1560:1560	arg1	retention					1562:1570	∼96.1% retention	1555:1570	∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1	1555:1634	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	0	105	theme	hierarchical	44:55	arg1	carbons					64:70	interconnected 3D hierarchical porous carbons	26:70	interconnected 3D hierarchical porous carbons from bacterial cellulose-based composite monoliths for supercapacitors	26:141	Controlled preparation of interconnected 3D hierarchical porous carbons from bacterial cellulose-based composite monoliths for supercapacitors.
32643739	4	106	theme	favorable	1074:1082	arg1	mesopores					1084:1092	favorable mesopores	1074:1092	favorable mesopores	1074:1092	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	5	107	theme	g-1	1434:1436	arg1	capacitance					1413:1423	a high specific capacitance	1397:1423	a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability	1397:1548	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	4	108	theme	porous	1031:1036	arg1	structure					1038:1046	a hierarchical porous structure	1016:1046	a hierarchical porous structure	1016:1046	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	0	109	theme	carbons	64:70	arg1	preparation					11:21	preparation	11:21	preparation of interconnected 3D hierarchical porous carbons from bacterial cellulose-based composite monoliths for supercapacitors	11:141	Controlled preparation of interconnected 3D hierarchical porous carbons from bacterial cellulose-based composite monoliths for supercapacitors.
32643739	7	110	theme	promising	1894:1902	arg1	applications					1904:1915	promising applications	1894:1915	promising applications in supercapacitors	1894:1934	The good electrochemical performance and facile effective synthesis of bio-derived carbon materials with tunable porous structures indicate promising applications in supercapacitors.
32643739	5	111	theme	cycling	1532:1538	arg1	stability					1540:1548	good cycling stability	1527:1548	good cycling stability	1527:1548	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	2	112	theme	porous	360:365	arg1	HPCs					376:379	HPCs	376:379	HPCs	376:379	Here, hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite have been successfully prepared by thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation.
32643739	2	112	theme	porous	360:365	arg1	carbons					367:373	hierarchical porous carbons	347:373	hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite	347:496	Here, hierarchical porous carbons (HPCs) with an interconnected three-dimensional morphology derived from a natural-based bacterial cellulose (BC) composite have been successfully prepared by thermally induced phase separation of poly(ethylene-co-vinyl alcohol) (EVOH) and subsequent carbonization/activation.
32643739	5	113	from	g-1	1447:1449	arg1	capacitance					1413:1423	a high specific capacitance	1397:1423	a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability	1397:1548	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	5	113	from	g-1	1447:1449	arg1	g-1					1434:1436	420 F g-1	1428:1436	420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability	1428:1548	Due to the synergistic effects of these features, the obtained porous carbon electrode used in a supercapacitor shows a high specific capacitance of 420 F g-1 at 0.5 A g-1, excellent rate performance with 75% capacitance retention at 20 A g-1, and good cycling stability with ∼96.1% retention even after 10 000 continuous charge-discharge cycles at 5 A g-1.
32643739	4	114	theme	g-1	1144:1146	arg1	SSA					1129:1131	a high SSA	1122:1131	a high SSA of 2161 m2 g-1	1122:1146	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	4	114	theme	g-1	1144:1146	arg1	micropores					1062:1071	moderate micropores	1053:1071	moderate micropores	1053:1071	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	4	114	theme	g-1	1144:1146	arg1	content					1176:1182	a maximum oxygen-dopant content	1152:1182	a maximum oxygen-dopant content of 9.99%	1152:1191	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	4	114	theme	g-1	1144:1146	arg1	macropores					1110:1119	interconnected macropores	1095:1119	interconnected macropores	1095:1119	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	4	114	theme	g-1	1144:1146	arg1	mesopores					1084:1092	favorable mesopores	1074:1092	favorable mesopores	1074:1092	The obtained porous carbon material possesses a hierarchical porous structure with moderate micropores, favorable mesopores, interconnected macropores, a high SSA of 2161 m2 g-1 and a maximum oxygen-dopant content of 9.99%, enabling an increase in the active materials utilization efficiency and wettability.
32643739	1	115	theme	specific	260:267	arg1	SSA					283:285	SSA	283:285	SSA	283:285	The controlled design and synthesis of porous carbons with anticipated microstructures and morphologies, and a high specific surface area (SSA) have been focused on for supercapacitor development.
32643739	1	115	theme	specific	260:267	arg1	area					277:280	a high specific surface area	253:280	a high specific surface area (SSA)	253:286	The controlled design and synthesis of porous carbons with anticipated microstructures and morphologies, and a high specific surface area (SSA) have been focused on for supercapacitor development.
32643739	7	116	theme	tunable	1859:1865	arg1	structures					1874:1883	tunable porous structures	1859:1883	tunable porous structures	1859:1883	The good electrochemical performance and facile effective synthesis of bio-derived carbon materials with tunable porous structures indicate promising applications in supercapacitors.
32151816	0	0	theme	pressure	79:86	arg1	forces					96:101	different osmotic and hydraulic pressure driving forces	47:101	different osmotic and hydraulic pressure driving forces	47:101	Fouling propensity of novel TFC membranes with different osmotic and hydraulic pressure driving forces.
32151816	4	1	theme	constant	701:708	arg1	pressure					718:725	a constant osmotic pressure	699:725	a constant osmotic pressure	699:725	Compared to the widely employed method of using a non-constant osmotic pressure difference during bench-scale fouling experiments, maintaining a constant osmotic pressure led to 50% more alginate deposited on the same membrane surface (from 13.7 to 21.7 g/m2).
32151816	0	2	theme	hydraulic	69:77	arg1	forces					96:101	different osmotic and hydraulic pressure driving forces	47:101	different osmotic and hydraulic pressure driving forces	47:101	Fouling propensity of novel TFC membranes with different osmotic and hydraulic pressure driving forces.
32151816	7	3	theme	layer	1376:1380	arg1	compaction					1382:1391	cake layer compaction	1371:1391	cake layer compaction	1371:1391	A detailed analysis of the model however demonstrated that it needs further development, incorporating pore size, porosity and tortuosity of the foulant cake to enable drawing reliable conclusions on the causality of cake layer compaction.
32151816	6	4	theme	structural	1059:1068	arg1	parameter					1070:1078	the cake structural parameter	1050:1078	the cake structural parameter	1050:1078	An applied hydraulic pressure of 1 bar already changed fouling cake deposition and the cake structural parameter shrunk by 224 and 83 μm for the two thin-film composite membranes tested.
32151816	2	5	theme	pressure	323:330	arg1	impacts					295:301	the impacts	291:301	the impacts of constant osmotic pressure and hydraulic pressure driving forces on membrane fouling	291:388	In this study, the impacts of constant osmotic pressure and hydraulic pressure driving forces on membrane fouling were investigated using a novel approach.
32151816	6	6	theme	cake	1054:1057	arg1	parameter					1070:1078	the cake structural parameter	1050:1078	the cake structural parameter	1050:1078	An applied hydraulic pressure of 1 bar already changed fouling cake deposition and the cake structural parameter shrunk by 224 and 83 μm for the two thin-film composite membranes tested.
32151816	5	7	theme	Na+	957:959	arg1	ions					961:964	Na+ ions	957:964	Na+ ions	957:964	This was attributed to a stronger osmotic driving force at the active layer interface and enhanced fouling due to a greater reverse flux of Na+ ions.
32151816	2	8	from	impacts	295:301	arg1	fouling					382:388	membrane fouling	373:388	membrane fouling	373:388	In this study, the impacts of constant osmotic pressure and hydraulic pressure driving forces on membrane fouling were investigated using a novel approach.
32151816	4	9	theme	bench-scale	654:664	arg1	experiments					674:684	bench-scale fouling experiments	654:684	bench-scale fouling experiments	654:684	Compared to the widely employed method of using a non-constant osmotic pressure difference during bench-scale fouling experiments, maintaining a constant osmotic pressure led to 50% more alginate deposited on the same membrane surface (from 13.7 to 21.7 g/m2).
32151816	1	10	theme	reported	241:248	arg1	propensity					264:273	lower fouling propensity	250:273	its reported lower fouling propensity	237:273	The feasibility of Forward Osmosis (FO) as an alternative treatment technology to current membrane processes is believed to hinge on its reported lower fouling propensity.
32151816	0	11	theme	driving	88:94	arg1	forces					96:101	different osmotic and hydraulic pressure driving forces	47:101	different osmotic and hydraulic pressure driving forces	47:101	Fouling propensity of novel TFC membranes with different osmotic and hydraulic pressure driving forces.
32151816	3	12	theme	effective	531:539	arg1	force					549:553	effective driving force	531:553	effective driving force	531:553	In each case the cake layer was modelled accounting for all concentration polarisation effects and effective driving force.
32151816	2	13	dep	pressure	323:330	arg1	forces					363:368	driving forces	355:368	driving forces	355:368	In this study, the impacts of constant osmotic pressure and hydraulic pressure driving forces on membrane fouling were investigated using a novel approach.
32151816	1	14	theme	lower	250:254	arg1	propensity					264:273	lower fouling propensity	250:273	its reported lower fouling propensity	237:273	The feasibility of Forward Osmosis (FO) as an alternative treatment technology to current membrane processes is believed to hinge on its reported lower fouling propensity.
32151816	5	15	theme	active	880:885	arg1	interface					893:901	the active layer interface	876:901	the active layer interface	876:901	This was attributed to a stronger osmotic driving force at the active layer interface and enhanced fouling due to a greater reverse flux of Na+ ions.
32151816	4	16	theme	non-constant	606:617	arg1	difference					636:645	a non-constant osmotic pressure difference	604:645	a non-constant osmotic pressure difference	604:645	Compared to the widely employed method of using a non-constant osmotic pressure difference during bench-scale fouling experiments, maintaining a constant osmotic pressure led to 50% more alginate deposited on the same membrane surface (from 13.7 to 21.7 g/m2).
32151816	3	17	theme	polarisation	506:517	arg1	effects					519:525	all concentration polarisation effects	488:525	all concentration polarisation effects	488:525	In each case the cake layer was modelled accounting for all concentration polarisation effects and effective driving force.
32151816	5	18	theme	stronger	842:849	arg1	force					867:871	a stronger osmotic driving force	840:871	a stronger osmotic driving force at the active layer interface	840:901	This was attributed to a stronger osmotic driving force at the active layer interface and enhanced fouling due to a greater reverse flux of Na+ ions.
32151816	2	19	theme	membrane	373:380	arg1	fouling					382:388	membrane fouling	373:388	membrane fouling	373:388	In this study, the impacts of constant osmotic pressure and hydraulic pressure driving forces on membrane fouling were investigated using a novel approach.
32151816	5	20	from	interface	893:901	arg1	force					867:871	a stronger osmotic driving force	840:871	a stronger osmotic driving force at the active layer interface	840:901	This was attributed to a stronger osmotic driving force at the active layer interface and enhanced fouling due to a greater reverse flux of Na+ ions.
32151816	6	21	theme	thin-film	1116:1124	arg1	membranes					1136:1144	the two thin-film composite membranes	1108:1144	the two thin-film composite membranes tested	1108:1151	An applied hydraulic pressure of 1 bar already changed fouling cake deposition and the cake structural parameter shrunk by 224 and 83 μm for the two thin-film composite membranes tested.
32151816	1	22	theme	alternative	150:160	arg1	technology					172:181	an alternative treatment technology	147:181	an alternative treatment technology to current membrane processes	147:211	The feasibility of Forward Osmosis (FO) as an alternative treatment technology to current membrane processes is believed to hinge on its reported lower fouling propensity.
32151816	3	23	theme	concentration	492:504	arg1	effects					519:525	all concentration polarisation effects	488:525	all concentration polarisation effects	488:525	In each case the cake layer was modelled accounting for all concentration polarisation effects and effective driving force.
32151816	7	24	theme	pore	1257:1260	arg1	size					1262:1265	pore size	1257:1265	pore size	1257:1265	A detailed analysis of the model however demonstrated that it needs further development, incorporating pore size, porosity and tortuosity of the foulant cake to enable drawing reliable conclusions on the causality of cake layer compaction.
32151816	7	25	theme	detailed	1156:1163	arg1	analysis					1165:1172	A detailed analysis	1154:1172	A detailed analysis of the model	1154:1185	A detailed analysis of the model however demonstrated that it needs further development, incorporating pore size, porosity and tortuosity of the foulant cake to enable drawing reliable conclusions on the causality of cake layer compaction.
32151816	6	26	theme	cake	1030:1033	arg1	deposition					1035:1044	1 bar already changed fouling cake deposition	1000:1044	1 bar already changed fouling cake deposition	1000:1044	An applied hydraulic pressure of 1 bar already changed fouling cake deposition and the cake structural parameter shrunk by 224 and 83 μm for the two thin-film composite membranes tested.
32151816	1	27	theme	treatment	162:170	arg1	technology					172:181	an alternative treatment technology	147:181	an alternative treatment technology to current membrane processes	147:211	The feasibility of Forward Osmosis (FO) as an alternative treatment technology to current membrane processes is believed to hinge on its reported lower fouling propensity.
32151816	0	28	theme	Fouling	0:6	arg1	propensity					8:17	Fouling propensity	0:17	Fouling propensity of novel TFC membranes with different osmotic and hydraulic pressure driving forces.	0:102	Fouling propensity of novel TFC membranes with different osmotic and hydraulic pressure driving forces.
32151816	1	29	theme	fouling	256:262	arg1	propensity					264:273	lower fouling propensity	250:273	its reported lower fouling propensity	237:273	The feasibility of Forward Osmosis (FO) as an alternative treatment technology to current membrane processes is believed to hinge on its reported lower fouling propensity.
32151816	7	30	theme	cake	1371:1374	arg1	compaction					1382:1391	cake layer compaction	1371:1391	cake layer compaction	1371:1391	A detailed analysis of the model however demonstrated that it needs further development, incorporating pore size, porosity and tortuosity of the foulant cake to enable drawing reliable conclusions on the causality of cake layer compaction.
32151816	2	31	theme	driving	355:361	arg1	forces					363:368	driving forces	355:368	driving forces	355:368	In this study, the impacts of constant osmotic pressure and hydraulic pressure driving forces on membrane fouling were investigated using a novel approach.
32151816	0	32	theme	novel	22:26	arg1	membranes					32:40	novel TFC membranes	22:40	novel TFC membranes	22:40	Fouling propensity of novel TFC membranes with different osmotic and hydraulic pressure driving forces.
32151816	6	33	theme	deposition	1035:1044	arg1	parameter					1070:1078	the cake structural parameter	1050:1078	the cake structural parameter	1050:1078	An applied hydraulic pressure of 1 bar already changed fouling cake deposition and the cake structural parameter shrunk by 224 and 83 μm for the two thin-film composite membranes tested.
32151816	6	33	theme	deposition	1035:1044	arg1	pressure					988:995	An applied hydraulic pressure	967:995	An applied hydraulic pressure of 1 bar already changed fouling cake deposition	967:1044	An applied hydraulic pressure of 1 bar already changed fouling cake deposition and the cake structural parameter shrunk by 224 and 83 μm for the two thin-film composite membranes tested.
32151816	4	34	from	deposited	752:760	arg1	surface					783:789	the same membrane surface	765:789	the same membrane surface (from 13.7 to 21.7 g/m2)	765:814	Compared to the widely employed method of using a non-constant osmotic pressure difference during bench-scale fouling experiments, maintaining a constant osmotic pressure led to 50% more alginate deposited on the same membrane surface (from 13.7 to 21.7 g/m2).
32151816	3	35	theme	driving	541:547	arg1	force					549:553	effective driving force	531:553	effective driving force	531:553	In each case the cake layer was modelled accounting for all concentration polarisation effects and effective driving force.
32151816	4	36	theme	osmotic	619:625	arg1	difference					636:645	a non-constant osmotic pressure difference	604:645	a non-constant osmotic pressure difference	604:645	Compared to the widely employed method of using a non-constant osmotic pressure difference during bench-scale fouling experiments, maintaining a constant osmotic pressure led to 50% more alginate deposited on the same membrane surface (from 13.7 to 21.7 g/m2).
32151816	5	37	theme	layer	887:891	arg1	interface					893:901	the active layer interface	876:901	the active layer interface	876:901	This was attributed to a stronger osmotic driving force at the active layer interface and enhanced fouling due to a greater reverse flux of Na+ ions.
32151816	4	38	theme	same	769:772	arg1	surface					783:789	the same membrane surface	765:789	the same membrane surface (from 13.7 to 21.7 g/m2)	765:814	Compared to the widely employed method of using a non-constant osmotic pressure difference during bench-scale fouling experiments, maintaining a constant osmotic pressure led to 50% more alginate deposited on the same membrane surface (from 13.7 to 21.7 g/m2).
32151816	4	39	theme	more	738:741	arg1	deposited					752:760	50% more alginate deposited	734:760	50% more alginate deposited on the same membrane surface (from 13.7 to 21.7 g/m2)	734:814	Compared to the widely employed method of using a non-constant osmotic pressure difference during bench-scale fouling experiments, maintaining a constant osmotic pressure led to 50% more alginate deposited on the same membrane surface (from 13.7 to 21.7 g/m2).
32151816	1	40	theme	current	186:192	arg1	processes					203:211	current membrane processes	186:211	current membrane processes	186:211	The feasibility of Forward Osmosis (FO) as an alternative treatment technology to current membrane processes is believed to hinge on its reported lower fouling propensity.
32151816	0	41	theme	membranes	32:40	arg1	propensity					8:17	Fouling propensity	0:17	Fouling propensity of novel TFC membranes with different osmotic and hydraulic pressure driving forces.	0:102	Fouling propensity of novel TFC membranes with different osmotic and hydraulic pressure driving forces.
32151816	4	42	theme	osmotic	710:716	arg1	pressure					718:725	a constant osmotic pressure	699:725	a constant osmotic pressure	699:725	Compared to the widely employed method of using a non-constant osmotic pressure difference during bench-scale fouling experiments, maintaining a constant osmotic pressure led to 50% more alginate deposited on the same membrane surface (from 13.7 to 21.7 g/m2).
32151816	6	43	theme	1 bar	1000:1004	arg1	deposition					1035:1044	1 bar already changed fouling cake deposition	1000:1044	1 bar already changed fouling cake deposition	1000:1044	An applied hydraulic pressure of 1 bar already changed fouling cake deposition and the cake structural parameter shrunk by 224 and 83 μm for the two thin-film composite membranes tested.
32151816	4	44	theme	fouling	666:672	arg1	experiments					674:684	bench-scale fouling experiments	654:684	bench-scale fouling experiments	654:684	Compared to the widely employed method of using a non-constant osmotic pressure difference during bench-scale fouling experiments, maintaining a constant osmotic pressure led to 50% more alginate deposited on the same membrane surface (from 13.7 to 21.7 g/m2).
32151816	1	45	theme	membrane	194:201	arg1	processes					203:211	current membrane processes	186:211	current membrane processes	186:211	The feasibility of Forward Osmosis (FO) as an alternative treatment technology to current membrane processes is believed to hinge on its reported lower fouling propensity.
32151816	0	46	theme	TFC	28:30	arg1	membranes					32:40	novel TFC membranes	22:40	novel TFC membranes	22:40	Fouling propensity of novel TFC membranes with different osmotic and hydraulic pressure driving forces.
32151816	2	47	theme	novel	416:420	arg1	approach					422:429	a novel approach	414:429	a novel approach	414:429	In this study, the impacts of constant osmotic pressure and hydraulic pressure driving forces on membrane fouling were investigated using a novel approach.
32151816	4	48	theme	pressure	627:634	arg1	difference					636:645	a non-constant osmotic pressure difference	604:645	a non-constant osmotic pressure difference	604:645	Compared to the widely employed method of using a non-constant osmotic pressure difference during bench-scale fouling experiments, maintaining a constant osmotic pressure led to 50% more alginate deposited on the same membrane surface (from 13.7 to 21.7 g/m2).
32151816	0	49	theme	different	47:55	arg1	forces					96:101	different osmotic and hydraulic pressure driving forces	47:101	different osmotic and hydraulic pressure driving forces	47:101	Fouling propensity of novel TFC membranes with different osmotic and hydraulic pressure driving forces.
32151816	5	50	theme	driving	859:865	arg1	force					867:871	a stronger osmotic driving force	840:871	a stronger osmotic driving force at the active layer interface	840:901	This was attributed to a stronger osmotic driving force at the active layer interface and enhanced fouling due to a greater reverse flux of Na+ ions.
32151816	7	51	theme	foulant	1299:1305	arg1	cake					1307:1310	the foulant cake	1295:1310	the foulant cake	1295:1310	A detailed analysis of the model however demonstrated that it needs further development, incorporating pore size, porosity and tortuosity of the foulant cake to enable drawing reliable conclusions on the causality of cake layer compaction.
32151816	5	52	theme	greater	933:939	arg1	flux					949:952	a greater reverse flux	931:952	a greater reverse flux of Na+ ions	931:964	This was attributed to a stronger osmotic driving force at the active layer interface and enhanced fouling due to a greater reverse flux of Na+ ions.
32151816	4	53	from	21.7 g/m2	805:813	arg1	surface					783:789	the same membrane surface	765:789	the same membrane surface (from 13.7 to 21.7 g/m2)	765:814	Compared to the widely employed method of using a non-constant osmotic pressure difference during bench-scale fouling experiments, maintaining a constant osmotic pressure led to 50% more alginate deposited on the same membrane surface (from 13.7 to 21.7 g/m2).
32151816	2	54	theme	osmotic	315:321	arg1	pressure					323:330	constant osmotic pressure	306:330	constant osmotic pressure	306:330	In this study, the impacts of constant osmotic pressure and hydraulic pressure driving forces on membrane fouling were investigated using a novel approach.
32151816	6	55	theme	fouling	1022:1028	arg1	deposition					1035:1044	1 bar already changed fouling cake deposition	1000:1044	1 bar already changed fouling cake deposition	1000:1044	An applied hydraulic pressure of 1 bar already changed fouling cake deposition and the cake structural parameter shrunk by 224 and 83 μm for the two thin-film composite membranes tested.
32151816	4	56	dep	21.7 g/m2	805:813	arg1	to					802:803	to	802:803	to	802:803	Compared to the widely employed method of using a non-constant osmotic pressure difference during bench-scale fouling experiments, maintaining a constant osmotic pressure led to 50% more alginate deposited on the same membrane surface (from 13.7 to 21.7 g/m2).
32151816	4	57	theme	employed	579:586	arg1	method					588:593	the widely employed method	568:593	the widely employed method of using a non-constant osmotic pressure difference during bench-scale fouling experiments	568:684	Compared to the widely employed method of using a non-constant osmotic pressure difference during bench-scale fouling experiments, maintaining a constant osmotic pressure led to 50% more alginate deposited on the same membrane surface (from 13.7 to 21.7 g/m2).
32151816	7	58	theme	reliable	1330:1337	arg1	conclusions					1339:1349	reliable conclusions	1330:1349	reliable conclusions	1330:1349	A detailed analysis of the model however demonstrated that it needs further development, incorporating pore size, porosity and tortuosity of the foulant cake to enable drawing reliable conclusions on the causality of cake layer compaction.
32151816	0	59	with	propensity	8:17	arg1	forces					96:101	different osmotic and hydraulic pressure driving forces	47:101	different osmotic and hydraulic pressure driving forces	47:101	Fouling propensity of novel TFC membranes with different osmotic and hydraulic pressure driving forces.
32151816	2	60	theme	constant	306:313	arg1	pressure					323:330	constant osmotic pressure	306:330	constant osmotic pressure	306:330	In this study, the impacts of constant osmotic pressure and hydraulic pressure driving forces on membrane fouling were investigated using a novel approach.
32151816	5	61	theme	osmotic	851:857	arg1	force					867:871	a stronger osmotic driving force	840:871	a stronger osmotic driving force at the active layer interface	840:901	This was attributed to a stronger osmotic driving force at the active layer interface and enhanced fouling due to a greater reverse flux of Na+ ions.
32151816	6	62	theme	hydraulic	978:986	arg1	pressure					988:995	An applied hydraulic pressure	967:995	An applied hydraulic pressure of 1 bar already changed fouling cake deposition	967:1044	An applied hydraulic pressure of 1 bar already changed fouling cake deposition and the cake structural parameter shrunk by 224 and 83 μm for the two thin-film composite membranes tested.
32151816	1	63	theme	Forward	123:129	arg1	FO					140:141	FO	140:141	FO	140:141	The feasibility of Forward Osmosis (FO) as an alternative treatment technology to current membrane processes is believed to hinge on its reported lower fouling propensity.
32151816	1	63	theme	Forward	123:129	arg1	Osmosis					131:137	Forward Osmosis	123:137	Forward Osmosis (FO)	123:142	The feasibility of Forward Osmosis (FO) as an alternative treatment technology to current membrane processes is believed to hinge on its reported lower fouling propensity.
32151816	4	64	theme	alginate	743:750	arg1	deposited					752:760	50% more alginate deposited	734:760	50% more alginate deposited on the same membrane surface (from 13.7 to 21.7 g/m2)	734:814	Compared to the widely employed method of using a non-constant osmotic pressure difference during bench-scale fouling experiments, maintaining a constant osmotic pressure led to 50% more alginate deposited on the same membrane surface (from 13.7 to 21.7 g/m2).
32151816	7	65	theme	compaction	1382:1391	arg1	causality					1358:1366	the causality	1354:1366	the causality of cake layer compaction	1354:1391	A detailed analysis of the model however demonstrated that it needs further development, incorporating pore size, porosity and tortuosity of the foulant cake to enable drawing reliable conclusions on the causality of cake layer compaction.
32151816	4	66	theme	membrane	774:781	arg1	surface					783:789	the same membrane surface	765:789	the same membrane surface (from 13.7 to 21.7 g/m2)	765:814	Compared to the widely employed method of using a non-constant osmotic pressure difference during bench-scale fouling experiments, maintaining a constant osmotic pressure led to 50% more alginate deposited on the same membrane surface (from 13.7 to 21.7 g/m2).
32151816	1	67	theme	Osmosis	131:137	arg1	feasibility					108:118	The feasibility	104:118	The feasibility of Forward Osmosis (FO) as an alternative treatment technology to current membrane processes	104:211	The feasibility of Forward Osmosis (FO) as an alternative treatment technology to current membrane processes is believed to hinge on its reported lower fouling propensity.
32151816	3	68	theme	cake	449:452	arg1	layer					454:458	the cake layer	445:458	the cake layer	445:458	In each case the cake layer was modelled accounting for all concentration polarisation effects and effective driving force.
32151816	7	69	theme	model	1181:1185	arg1	analysis					1165:1172	A detailed analysis	1154:1172	A detailed analysis of the model	1154:1185	A detailed analysis of the model however demonstrated that it needs further development, incorporating pore size, porosity and tortuosity of the foulant cake to enable drawing reliable conclusions on the causality of cake layer compaction.
32151816	5	70	theme	reverse	941:947	arg1	flux					949:952	a greater reverse flux	931:952	a greater reverse flux of Na+ ions	931:964	This was attributed to a stronger osmotic driving force at the active layer interface and enhanced fouling due to a greater reverse flux of Na+ ions.
32151816	5	71	theme	ions	961:964	arg1	flux					949:952	a greater reverse flux	931:952	a greater reverse flux of Na+ ions	931:964	This was attributed to a stronger osmotic driving force at the active layer interface and enhanced fouling due to a greater reverse flux of Na+ ions.
32151816	6	72	theme	changed	1014:1020	arg1	deposition					1035:1044	1 bar already changed fouling cake deposition	1000:1044	1 bar already changed fouling cake deposition	1000:1044	An applied hydraulic pressure of 1 bar already changed fouling cake deposition and the cake structural parameter shrunk by 224 and 83 μm for the two thin-film composite membranes tested.
32151816	2	73	theme	pressure	346:353	arg1	impacts					295:301	the impacts	291:301	the impacts of constant osmotic pressure and hydraulic pressure driving forces on membrane fouling	291:388	In this study, the impacts of constant osmotic pressure and hydraulic pressure driving forces on membrane fouling were investigated using a novel approach.
32151816	6	74	theme	composite	1126:1134	arg1	membranes					1136:1144	the two thin-film composite membranes	1108:1144	the two thin-film composite membranes tested	1108:1151	An applied hydraulic pressure of 1 bar already changed fouling cake deposition and the cake structural parameter shrunk by 224 and 83 μm for the two thin-film composite membranes tested.
32151816	6	75	theme	applied	970:976	arg1	pressure					988:995	An applied hydraulic pressure	967:995	An applied hydraulic pressure of 1 bar already changed fouling cake deposition	967:1044	An applied hydraulic pressure of 1 bar already changed fouling cake deposition and the cake structural parameter shrunk by 224 and 83 μm for the two thin-film composite membranes tested.
32151816	0	76	theme	osmotic	57:63	arg1	forces					96:101	different osmotic and hydraulic pressure driving forces	47:101	different osmotic and hydraulic pressure driving forces	47:101	Fouling propensity of novel TFC membranes with different osmotic and hydraulic pressure driving forces.
32151816	7	77	theme	cake	1307:1310	arg1	tortuosity					1281:1290	tortuosity	1281:1290	tortuosity	1281:1290	A detailed analysis of the model however demonstrated that it needs further development, incorporating pore size, porosity and tortuosity of the foulant cake to enable drawing reliable conclusions on the causality of cake layer compaction.
32151816	7	77	theme	cake	1307:1310	arg1	porosity					1268:1275	porosity	1268:1275	porosity	1268:1275	A detailed analysis of the model however demonstrated that it needs further development, incorporating pore size, porosity and tortuosity of the foulant cake to enable drawing reliable conclusions on the causality of cake layer compaction.
32151816	7	77	theme	cake	1307:1310	arg1	size					1262:1265	pore size	1257:1265	pore size	1257:1265	A detailed analysis of the model however demonstrated that it needs further development, incorporating pore size, porosity and tortuosity of the foulant cake to enable drawing reliable conclusions on the causality of cake layer compaction.
32151816	2	78	theme	hydraulic	336:344	arg1	pressure					346:353	hydraulic pressure	336:353	hydraulic pressure	336:353	In this study, the impacts of constant osmotic pressure and hydraulic pressure driving forces on membrane fouling were investigated using a novel approach.
34669255	8	0	theme	ether	933:937	arg1	isomer					939:944	davana ether isomer	926:944	davana ether isomer (3.4 %)	926:952	Major components of the DO were cis-davanone (D3, 53.0 %), bicyclogermacrene (6.9 %), trans-ethyl cinnamate (4.9 %), davana ether isomer (3.4 %), spathulenol (2.8 %), cis-hydroxy davanone (2.4 %), and trans-davanone (2.1 %).
34669255	8	0	theme	ether	933:937	arg1	%					951:951	3.4 %	947:951	3.4 %	947:951	Major components of the DO were cis-davanone (D3, 53.0 %), bicyclogermacrene (6.9 %), trans-ethyl cinnamate (4.9 %), davana ether isomer (3.4 %), spathulenol (2.8 %), cis-hydroxy davanone (2.4 %), and trans-davanone (2.1 %).
34669255	12	1	theme	concentrations	1650:1663	arg1	administration					1616:1629	administration	1616:1629	administration of their effective concentrations	1616:1663	Pharmacological profile revealed that the treatment of DO and D3 inhibited the production of pro-inflammatory cytokines (TNF-α, IL-6) induced by lipopolysaccharide (LPS) in primary macrophages without any cytotoxic effect after administration of their effective concentrations.
34669255	5	2	theme	davana	403:408	arg1	oil					420:422	davana essential oil	403:422	davana essential oil (DO)	403:427	The compositional complexity of davana essential oil (DO) has been a challenge for quality control.
34669255	6	3	theme	chemical	490:497	arg1	profile					499:505	the chemical profile	486:505	the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques	486:741	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	8	4	theme	davana	926:931	arg1	isomer					939:944	davana ether isomer	926:944	davana ether isomer (3.4 %)	926:952	Major components of the DO were cis-davanone (D3, 53.0 %), bicyclogermacrene (6.9 %), trans-ethyl cinnamate (4.9 %), davana ether isomer (3.4 %), spathulenol (2.8 %), cis-hydroxy davanone (2.4 %), and trans-davanone (2.1 %).
34669255	8	4	theme	davana	926:931	arg1	%					951:951	3.4 %	947:951	3.4 %	947:951	Major components of the DO were cis-davanone (D3, 53.0 %), bicyclogermacrene (6.9 %), trans-ethyl cinnamate (4.9 %), davana ether isomer (3.4 %), spathulenol (2.8 %), cis-hydroxy davanone (2.4 %), and trans-davanone (2.1 %).
34669255	8	5	theme	trans-ethyl	895:905	arg1	cinnamate					907:915	trans-ethyl cinnamate	895:915	trans-ethyl cinnamate (4.9 %)	895:923	Major components of the DO were cis-davanone (D3, 53.0 %), bicyclogermacrene (6.9 %), trans-ethyl cinnamate (4.9 %), davana ether isomer (3.4 %), spathulenol (2.8 %), cis-hydroxy davanone (2.4 %), and trans-davanone (2.1 %).
34669255	8	5	theme	trans-ethyl	895:905	arg1	%					922:922	4.9 %	918:922	4.9 %	918:922	Major components of the DO were cis-davanone (D3, 53.0 %), bicyclogermacrene (6.9 %), trans-ethyl cinnamate (4.9 %), davana ether isomer (3.4 %), spathulenol (2.8 %), cis-hydroxy davanone (2.4 %), and trans-davanone (2.1 %).
34669255	9	6	theme	novel	1082:1086	arg1	components					1094:1103	identifying several co-eluting novel minor components	1051:1103	identifying several co-eluting novel minor components	1051:1103	The study led to identifying several co-eluting novel minor components, which could help determine the authenticity of DO.
34669255	5	7	theme	essential	410:418	arg1	oil					420:422	davana essential oil	403:422	davana essential oil (DO)	403:427	The compositional complexity of davana essential oil (DO) has been a challenge for quality control.
34669255	9	8	theme	identifying	1051:1061	arg1	components					1094:1103	identifying several co-eluting novel minor components	1051:1103	identifying several co-eluting novel minor components	1051:1103	The study led to identifying several co-eluting novel minor components, which could help determine the authenticity of DO.
34669255	5	9	dep	oil	420:422	arg1	DO					425:426	DO	425:426	DO	425:426	The compositional complexity of davana essential oil (DO) has been a challenge for quality control.
34669255	10	10	theme	compounds	1221:1229	arg1	isolation					1203:1211	the isolation	1199:1211	the isolation of five compounds	1199:1229	The rigorous column-chromatography led to the isolation of five compounds.
34669255	12	11	dep	TNF-α	1509:1513	arg1	IL-6					1516:1519	IL-6	1516:1519	IL-6	1516:1519	Pharmacological profile revealed that the treatment of DO and D3 inhibited the production of pro-inflammatory cytokines (TNF-α, IL-6) induced by lipopolysaccharide (LPS) in primary macrophages without any cytotoxic effect after administration of their effective concentrations.
34669255	5	12	theme	oil	420:422	arg1	challenge					440:448	a challenge	438:448	a challenge for quality control	438:468	The compositional complexity of davana essential oil (DO) has been a challenge for quality control.
34669255	5	12	theme	oil	420:422	arg1	complexity					389:398	The compositional complexity	371:398	The compositional complexity of davana essential oil (DO)	371:427	The compositional complexity of davana essential oil (DO) has been a challenge for quality control.
34669255	12	13	theme	Pharmacological	1388:1402	arg1	profile					1404:1410	Pharmacological profile	1388:1410	Pharmacological profile	1388:1410	Pharmacological profile revealed that the treatment of DO and D3 inhibited the production of pro-inflammatory cytokines (TNF-α, IL-6) induced by lipopolysaccharide (LPS) in primary macrophages without any cytotoxic effect after administration of their effective concentrations.
34669255	13	14	theme	inflammatory	1790:1801	arg1	conditions					1803:1812	chronic skin inflammatory conditions	1777:1812	chronic skin inflammatory conditions	1777:1812	The result of this study indicates the suitability of DO and D3 for further investigation for the treatment of chronic skin inflammatory conditions.
34669255	13	15	theme	study	1685:1689	arg1	result					1670:1675	The result	1666:1675	The result of this study	1666:1689	The result of this study indicates the suitability of DO and D3 for further investigation for the treatment of chronic skin inflammatory conditions.
34669255	8	16	dep	D3	855:856	arg1	%					864:864	53.0 %	859:864	53.0 %	859:864	Major components of the DO were cis-davanone (D3, 53.0 %), bicyclogermacrene (6.9 %), trans-ethyl cinnamate (4.9 %), davana ether isomer (3.4 %), spathulenol (2.8 %), cis-hydroxy davanone (2.4 %), and trans-davanone (2.1 %).
34669255	1	17	dep	DC	127:128	arg1	ex					124:125	ex DC	124:128	ex DC	124:128	ex DC.)
34669255	5	18	theme	quality	454:460	arg1	control					462:468	quality control	454:468	quality control	454:468	The compositional complexity of davana essential oil (DO) has been a challenge for quality control.
34669255	13	19	theme	D3	1727:1728	arg1	suitability					1705:1715	the suitability	1701:1715	the suitability of DO and D3 for further investigation for the treatment of chronic skin inflammatory conditions	1701:1812	The result of this study indicates the suitability of DO and D3 for further investigation for the treatment of chronic skin inflammatory conditions.
34669255	4	20	theme	considerable	262:273	arg1	attention					275:283	considerable attention	262:283	considerable attention	262:283	ex DC., popularly known as davana, has gained considerable attention because of its unique fragrance, high economic value, and pharmacological properties.
34669255	6	21	dep	profile	499:505	arg1	was					513:515	was	513:515	DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques	510:741	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	6	21	dep	profile	499:505	arg1	study					479:483	this study	474:483	this study	474:483	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	6	22	theme	chromatographic	587:601	arg1	gas					583:585	gas chromatographic	583:601	gas chromatographic (GC-FID)	583:610	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	6	22	theme	chromatographic	587:601	arg1	GC-FID					604:609	GC-FID	604:609	GC-FID	604:609	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	13	23	theme	further	1734:1740	arg1	investigation					1742:1754	further investigation	1734:1754	further investigation	1734:1754	The result of this study indicates the suitability of DO and D3 for further investigation for the treatment of chronic skin inflammatory conditions.
34669255	6	24	theme	spectroscopic	653:665	arg1	techniques					732:741	spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques	653:741	spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques	653:741	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	0	25	theme	Artemisia	100:108	arg1	Profile					81:87	Pro-Inflammatory Cytokine Inhibition Profile	44:87	Pro-Inflammatory Cytokine Inhibition Profile of Davana (Artemisia	44:108	New Insights into the Chemical Composition, Pro-Inflammatory Cytokine Inhibition Profile of Davana (Artemisia pallens Wall.
34669255	0	25	theme	Artemisia	100:108	arg1	Composition					31:41	the Chemical Composition	18:41	the Chemical Composition	18:41	New Insights into the Chemical Composition, Pro-Inflammatory Cytokine Inhibition Profile of Davana (Artemisia pallens Wall.
34669255	9	26	theme	DO	1153:1154	arg1	authenticity					1137:1148	the authenticity	1133:1148	the authenticity of DO	1133:1154	The study led to identifying several co-eluting novel minor components, which could help determine the authenticity of DO.
34669255	10	27	theme	rigorous	1161:1168	arg1	column-chromatography					1170:1190	The rigorous column-chromatography	1157:1190	The rigorous column-chromatography	1157:1190	The rigorous column-chromatography led to the isolation of five compounds.
34669255	13	28	theme	skin	1785:1788	arg1	conditions					1803:1812	chronic skin inflammatory conditions	1777:1812	chronic skin inflammatory conditions	1777:1812	The result of this study indicates the suitability of DO and D3 for further investigation for the treatment of chronic skin inflammatory conditions.
34669255	11	29	theme	trans-ethyl	1264:1274	arg1	cinnamate					1276:1284	trans-ethyl cinnamate	1264:1284	trans-ethyl cinnamate	1264:1284	Among these, bicyclogermacrene, trans-ethyl cinnamate, and spathulenol were isolated and characterized by spectroscopic methods for the first time from DO.
34669255	13	30	theme	conditions	1803:1812	arg1	treatment					1764:1772	the treatment	1760:1772	the treatment of chronic skin inflammatory conditions	1760:1812	The result of this study indicates the suitability of DO and D3 for further investigation for the treatment of chronic skin inflammatory conditions.
34669255	6	31	dep	techniques	732:741	arg1	1D					697:698	1D	697:698	1D	697:698	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	6	31	dep	techniques	732:741	arg1	Infra-Red					686:694	Infra-Red	686:694	Infra-Red	686:694	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	6	31	dep	techniques	732:741	arg1	Resonance					721:729	2D-Nuclear Magnetic Resonance	701:729	2D-Nuclear Magnetic Resonance	701:729	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	0	32	theme	Inhibition	70:79	arg1	Profile					81:87	Pro-Inflammatory Cytokine Inhibition Profile	44:87	Pro-Inflammatory Cytokine Inhibition Profile of Davana (Artemisia	44:108	New Insights into the Chemical Composition, Pro-Inflammatory Cytokine Inhibition Profile of Davana (Artemisia pallens Wall.
34669255	0	32	theme	Inhibition	70:79	arg1	Composition					31:41	the Chemical Composition	18:41	the Chemical Composition	18:41	New Insights into the Chemical Composition, Pro-Inflammatory Cytokine Inhibition Profile of Davana (Artemisia pallens Wall.
34669255	2	33	theme	Essential	132:140	arg1	Oil					142:144	Essential Oil	132:144	Essential Oil	132:144	Essential Oil and cis-Davanone in Primary Macrophage Cells.
34669255	0	34	theme	New	0:2	arg1	Insights					4:11	New Insights	0:11	New Insights into the Chemical Composition, Pro-Inflammatory Cytokine Inhibition Profile of Davana (Artemisia	0:108	New Insights into the Chemical Composition, Pro-Inflammatory Cytokine Inhibition Profile of Davana (Artemisia pallens Wall.
34669255	13	35	theme	DO	1720:1721	arg1	suitability					1705:1715	the suitability	1701:1715	the suitability of DO and D3 for further investigation for the treatment of chronic skin inflammatory conditions	1701:1812	The result of this study indicates the suitability of DO and D3 for further investigation for the treatment of chronic skin inflammatory conditions.
34669255	6	36	theme	techniques	732:741	arg1	fractionation					548:560	polarity-based fractionation	533:560	polarity-based fractionation	533:560	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	6	36	theme	techniques	732:741	arg1	combination					568:578	a combination	566:578	a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques	566:741	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	7	37	theme	ninety-nine	786:796	arg1	compounds					798:806	ninety-nine compounds	786:806	ninety-nine compounds	786:806	The analysis led to the identification of ninety-nine compounds.
34669255	9	38	theme	several	1063:1069	arg1	components					1094:1103	identifying several co-eluting novel minor components	1051:1103	identifying several co-eluting novel minor components	1051:1103	The study led to identifying several co-eluting novel minor components, which could help determine the authenticity of DO.
34669255	12	39	theme	D3	1450:1451	arg1	treatment					1430:1438	the treatment	1426:1438	the treatment of DO and D3	1426:1451	Pharmacological profile revealed that the treatment of DO and D3 inhibited the production of pro-inflammatory cytokines (TNF-α, IL-6) induced by lipopolysaccharide (LPS) in primary macrophages without any cytotoxic effect after administration of their effective concentrations.
34669255	12	40	from	production	1467:1476	arg1	macrophages					1569:1579	primary macrophages	1561:1579	primary macrophages	1561:1579	Pharmacological profile revealed that the treatment of DO and D3 inhibited the production of pro-inflammatory cytokines (TNF-α, IL-6) induced by lipopolysaccharide (LPS) in primary macrophages without any cytotoxic effect after administration of their effective concentrations.
34669255	4	41	theme	pharmacological	343:357	arg1	properties					359:368	pharmacological properties	343:368	pharmacological properties	343:368	ex DC., popularly known as davana, has gained considerable attention because of its unique fragrance, high economic value, and pharmacological properties.
34669255	7	42	theme	compounds	798:806	arg1	identification					768:781	the identification	764:781	the identification of ninety-nine compounds	764:806	The analysis led to the identification of ninety-nine compounds.
34669255	6	43	dep	was	513:515	arg1	developed					517:525	developed	517:525	DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques	510:741	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	2	44	theme	Primary	166:172	arg1	Cells					185:189	Primary Macrophage Cells	166:189	Primary Macrophage Cells	166:189	Essential Oil and cis-Davanone in Primary Macrophage Cells.
34669255	12	45	theme	cytokines	1498:1506	arg1	production					1467:1476	the production	1463:1476	the production of pro-inflammatory cytokines (TNF-α, IL-6) induced by lipopolysaccharide (LPS) in primary macrophages	1463:1579	Pharmacological profile revealed that the treatment of DO and D3 inhibited the production of pro-inflammatory cytokines (TNF-α, IL-6) induced by lipopolysaccharide (LPS) in primary macrophages without any cytotoxic effect after administration of their effective concentrations.
34669255	12	46	theme	pro-inflammatory	1481:1496	arg1	cytokines					1498:1506	pro-inflammatory cytokines	1481:1506	pro-inflammatory cytokines (TNF-α, IL-6) induced by lipopolysaccharide (LPS)	1481:1556	Pharmacological profile revealed that the treatment of DO and D3 inhibited the production of pro-inflammatory cytokines (TNF-α, IL-6) induced by lipopolysaccharide (LPS) in primary macrophages without any cytotoxic effect after administration of their effective concentrations.
34669255	12	46	theme	pro-inflammatory	1481:1496	arg1	TNF-α					1509:1513	TNF-α	1509:1513	TNF-α	1509:1513	Pharmacological profile revealed that the treatment of DO and D3 inhibited the production of pro-inflammatory cytokines (TNF-α, IL-6) induced by lipopolysaccharide (LPS) in primary macrophages without any cytotoxic effect after administration of their effective concentrations.
34669255	6	47	theme	polarity-based	533:546	arg1	fractionation					548:560	polarity-based fractionation	533:560	polarity-based fractionation	533:560	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	8	48	theme	the	829:831	arg1	components					815:824	Major components	809:824	Major components of the DO	809:834	Major components of the DO were cis-davanone (D3, 53.0 %), bicyclogermacrene (6.9 %), trans-ethyl cinnamate (4.9 %), davana ether isomer (3.4 %), spathulenol (2.8 %), cis-hydroxy davanone (2.4 %), and trans-davanone (2.1 %).
34669255	4	49	theme	ex	216:217	arg1	DC.					219:221	ex DC.	216:221	ex DC.	216:221	ex DC., popularly known as davana, has gained considerable attention because of its unique fragrance, high economic value, and pharmacological properties.
34669255	6	50	theme	gas	583:585	arg1	fractionation					548:560	polarity-based fractionation	533:560	polarity-based fractionation	533:560	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	6	50	theme	gas	583:585	arg1	combination					568:578	a combination	566:578	a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques	566:741	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	6	51	theme	chromatographic	624:638	arg1	fractionation					548:560	polarity-based fractionation	533:560	polarity-based fractionation	533:560	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	6	51	theme	chromatographic	624:638	arg1	combination					568:578	a combination	566:578	a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques	566:741	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	0	52	theme	Chemical	22:29	arg1	Profile					81:87	Pro-Inflammatory Cytokine Inhibition Profile	44:87	Pro-Inflammatory Cytokine Inhibition Profile of Davana (Artemisia	44:108	New Insights into the Chemical Composition, Pro-Inflammatory Cytokine Inhibition Profile of Davana (Artemisia pallens Wall.
34669255	0	52	theme	Chemical	22:29	arg1	Composition					31:41	the Chemical Composition	18:41	the Chemical Composition	18:41	New Insights into the Chemical Composition, Pro-Inflammatory Cytokine Inhibition Profile of Davana (Artemisia pallens Wall.
34669255	9	53	theme	co-eluting	1071:1080	arg1	components					1094:1103	identifying several co-eluting novel minor components	1051:1103	identifying several co-eluting novel minor components	1051:1103	The study led to identifying several co-eluting novel minor components, which could help determine the authenticity of DO.
34669255	4	54	theme	high	318:321	arg1	value					332:336	high economic value	318:336	high economic value	318:336	ex DC., popularly known as davana, has gained considerable attention because of its unique fragrance, high economic value, and pharmacological properties.
34669255	12	55	theme	DO	1443:1444	arg1	treatment					1430:1438	the treatment	1426:1438	the treatment of DO and D3	1426:1451	Pharmacological profile revealed that the treatment of DO and D3 inhibited the production of pro-inflammatory cytokines (TNF-α, IL-6) induced by lipopolysaccharide (LPS) in primary macrophages without any cytotoxic effect after administration of their effective concentrations.
34669255	2	56	from	Oil	142:144	arg1	Cells					185:189	Primary Macrophage Cells	166:189	Primary Macrophage Cells	166:189	Essential Oil and cis-Davanone in Primary Macrophage Cells.
34669255	0	57	theme	Cytokine	61:68	arg1	Profile					81:87	Pro-Inflammatory Cytokine Inhibition Profile	44:87	Pro-Inflammatory Cytokine Inhibition Profile of Davana (Artemisia	44:108	New Insights into the Chemical Composition, Pro-Inflammatory Cytokine Inhibition Profile of Davana (Artemisia pallens Wall.
34669255	0	57	theme	Cytokine	61:68	arg1	Composition					31:41	the Chemical Composition	18:41	the Chemical Composition	18:41	New Insights into the Chemical Composition, Pro-Inflammatory Cytokine Inhibition Profile of Davana (Artemisia pallens Wall.
34669255	6	58	theme	Magnetic	712:719	arg1	Infra-Red					686:694	Infra-Red	686:694	Infra-Red	686:694	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	6	58	theme	Magnetic	712:719	arg1	Resonance					721:729	2D-Nuclear Magnetic Resonance	701:729	2D-Nuclear Magnetic Resonance	701:729	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	9	59	theme	minor	1088:1092	arg1	components					1094:1103	identifying several co-eluting novel minor components	1051:1103	identifying several co-eluting novel minor components	1051:1103	The study led to identifying several co-eluting novel minor components, which could help determine the authenticity of DO.
34669255	6	60	theme	2D-Nuclear	701:710	arg1	Infra-Red					686:694	Infra-Red	686:694	Infra-Red	686:694	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	6	60	theme	2D-Nuclear	701:710	arg1	Resonance					721:729	2D-Nuclear Magnetic Resonance	701:729	2D-Nuclear Magnetic Resonance	701:729	In this study, the chemical profile of DO was developed using polarity-based fractionation and a combination of gas chromatographic (GC-FID), hyphenated chromatographic (GC/MS), and spectroscopic (Fourier-Transform Infra-Red, 1D, 2D-Nuclear Magnetic Resonance) techniques.
34669255	11	61	theme	spectroscopic	1338:1350	arg1	methods					1352:1358	spectroscopic methods	1338:1358	spectroscopic methods for the first time from DO	1338:1385	Among these, bicyclogermacrene, trans-ethyl cinnamate, and spathulenol were isolated and characterized by spectroscopic methods for the first time from DO.
34669255	8	62	dep	the	829:831	arg1	DO					833:834	DO	833:834	DO	833:834	Major components of the DO were cis-davanone (D3, 53.0 %), bicyclogermacrene (6.9 %), trans-ethyl cinnamate (4.9 %), davana ether isomer (3.4 %), spathulenol (2.8 %), cis-hydroxy davanone (2.4 %), and trans-davanone (2.1 %).
34669255	4	63	theme	economic	323:330	arg1	value					332:336	high economic value	318:336	high economic value	318:336	ex DC., popularly known as davana, has gained considerable attention because of its unique fragrance, high economic value, and pharmacological properties.
34669255	8	64	theme	cis-hydroxy	976:986	arg1	%					1002:1002	2.4 %	998:1002	2.4 %	998:1002	Major components of the DO were cis-davanone (D3, 53.0 %), bicyclogermacrene (6.9 %), trans-ethyl cinnamate (4.9 %), davana ether isomer (3.4 %), spathulenol (2.8 %), cis-hydroxy davanone (2.4 %), and trans-davanone (2.1 %).
34669255	8	64	theme	cis-hydroxy	976:986	arg1	davanone					988:995	cis-hydroxy davanone	976:995	cis-hydroxy davanone (2.4 %)	976:1003	Major components of the DO were cis-davanone (D3, 53.0 %), bicyclogermacrene (6.9 %), trans-ethyl cinnamate (4.9 %), davana ether isomer (3.4 %), spathulenol (2.8 %), cis-hydroxy davanone (2.4 %), and trans-davanone (2.1 %).
34669255	12	65	theme	effective	1640:1648	arg1	concentrations					1650:1663	their effective concentrations	1634:1663	their effective concentrations	1634:1663	Pharmacological profile revealed that the treatment of DO and D3 inhibited the production of pro-inflammatory cytokines (TNF-α, IL-6) induced by lipopolysaccharide (LPS) in primary macrophages without any cytotoxic effect after administration of their effective concentrations.
34669255	12	66	theme	primary	1561:1567	arg1	macrophages					1569:1579	primary macrophages	1561:1579	primary macrophages	1561:1579	Pharmacological profile revealed that the treatment of DO and D3 inhibited the production of pro-inflammatory cytokines (TNF-α, IL-6) induced by lipopolysaccharide (LPS) in primary macrophages without any cytotoxic effect after administration of their effective concentrations.
34669255	2	67	from	cis-Davanone	150:161	arg1	Cells					185:189	Primary Macrophage Cells	166:189	Primary Macrophage Cells	166:189	Essential Oil and cis-Davanone in Primary Macrophage Cells.
34669255	2	68	theme	Macrophage	174:183	arg1	Cells					185:189	Primary Macrophage Cells	166:189	Primary Macrophage Cells	166:189	Essential Oil and cis-Davanone in Primary Macrophage Cells.
34669255	11	69	theme	first	1368:1372	arg1	time					1374:1377	the first time from DO	1364:1385	the first time from DO	1364:1385	Among these, bicyclogermacrene, trans-ethyl cinnamate, and spathulenol were isolated and characterized by spectroscopic methods for the first time from DO.
34669255	12	70	theme	cytotoxic	1593:1601	arg1	effect					1603:1608	any cytotoxic effect	1589:1608	any cytotoxic effect	1589:1608	Pharmacological profile revealed that the treatment of DO and D3 inhibited the production of pro-inflammatory cytokines (TNF-α, IL-6) induced by lipopolysaccharide (LPS) in primary macrophages without any cytotoxic effect after administration of their effective concentrations.
34669255	5	71	theme	compositional	375:387	arg1	challenge					440:448	a challenge	438:448	a challenge for quality control	438:468	The compositional complexity of davana essential oil (DO) has been a challenge for quality control.
34669255	5	71	theme	compositional	375:387	arg1	complexity					389:398	The compositional complexity	371:398	The compositional complexity of davana essential oil (DO)	371:427	The compositional complexity of davana essential oil (DO) has been a challenge for quality control.
34669255	0	72	theme	Pro-Inflammatory	44:59	arg1	Profile					81:87	Pro-Inflammatory Cytokine Inhibition Profile	44:87	Pro-Inflammatory Cytokine Inhibition Profile of Davana (Artemisia	44:108	New Insights into the Chemical Composition, Pro-Inflammatory Cytokine Inhibition Profile of Davana (Artemisia pallens Wall.
34669255	0	72	theme	Pro-Inflammatory	44:59	arg1	Composition					31:41	the Chemical Composition	18:41	the Chemical Composition	18:41	New Insights into the Chemical Composition, Pro-Inflammatory Cytokine Inhibition Profile of Davana (Artemisia pallens Wall.
34669255	13	73	theme	chronic	1777:1783	arg1	conditions					1803:1812	chronic skin inflammatory conditions	1777:1812	chronic skin inflammatory conditions	1777:1812	The result of this study indicates the suitability of DO and D3 for further investigation for the treatment of chronic skin inflammatory conditions.
34669255	8	74	theme	Major	809:813	arg1	components					815:824	Major components	809:824	Major components of the DO	809:834	Major components of the DO were cis-davanone (D3, 53.0 %), bicyclogermacrene (6.9 %), trans-ethyl cinnamate (4.9 %), davana ether isomer (3.4 %), spathulenol (2.8 %), cis-hydroxy davanone (2.4 %), and trans-davanone (2.1 %).
32088621	0	0	with	dancing	89:95	arg1	polymer					115:121	cyclodextrin polymer	102:121	cyclodextrin polymer	102:121	Integration of homogeneous and heterogeneous advanced oxidation processes: Confined iron dancing with cyclodextrin polymer.
32088621	1	1	theme	homogenous	176:185	arg1	reactions					194:202	heterogeneous and homogenous Fenton reactions	158:202	heterogeneous and homogenous Fenton reactions	158:202	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	1	2	with	polymer	371:377	arg1	EDTA					426:429	EDTA	426:429	EDTA	426:429	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	1	2	with	polymer	371:377	arg1	acid					420:423	ethylene diamine tetraacetic acid	391:423	ethylene diamine tetraacetic acid (EDTA)	391:430	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	0	3	theme	cyclodextrin	102:113	arg1	polymer					115:121	cyclodextrin polymer	102:121	cyclodextrin polymer	102:121	Integration of homogeneous and heterogeneous advanced oxidation processes: Confined iron dancing with cyclodextrin polymer.
32088621	5	4	theme	main	1053:1056	arg1	species					1077:1083	main reactive oxidizing species	1053:1083	main reactive oxidizing species	1053:1083	The quinone molecules 2,5-DBQ in the β-cyclodextrin cavity can accelerate Fe3+/Fe2+ cycle adjacent to the cavity, thus in favor of the decomposition of H2O2 into OH as main reactive oxidizing species.
32088621	3	5	theme	Mechanism	598:606	arg1	study					608:612	Mechanism study	598:612	Mechanism study	598:612	Mechanism study revealed that although the initial active site is Fe3O4 loaded on ECDP, the actually catalyst is the iron ions released from Fe3O4 but confined within the composite.
32088621	6	6	from	applications	1225:1236	arg1	promising					1202:1210	promising	1202:1210	promising	1202:1210	The current catalyst integrates the advantages of homogeneous and heterogeneous advanced oxidation processes and is promising in practical applications.
32088621	6	6	from	applications	1225:1236	arg1	catalyst					1098:1105	The current catalyst	1086:1105	The current catalyst	1086:1105	The current catalyst integrates the advantages of homogeneous and heterogeneous advanced oxidation processes and is promising in practical applications.
32088621	3	7	dep	ions	720:723	arg1	released					725:732	released	725:732	released from Fe3O4	725:743	Mechanism study revealed that although the initial active site is Fe3O4 loaded on ECDP, the actually catalyst is the iron ions released from Fe3O4 but confined within the composite.
32088621	3	7	dep	ions	720:723	arg1	confined					749:756	confined	749:756	confined within the composite	749:777	Mechanism study revealed that although the initial active site is Fe3O4 loaded on ECDP, the actually catalyst is the iron ions released from Fe3O4 but confined within the composite.
32088621	1	8	theme	ethylene	391:398	arg1	EDTA					426:429	EDTA	426:429	EDTA	426:429	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	1	8	theme	ethylene	391:398	arg1	acid					420:423	ethylene diamine tetraacetic acid	391:423	ethylene diamine tetraacetic acid (EDTA)	391:430	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	1	9	theme	Fenton	187:192	arg1	reactions					194:202	heterogeneous and homogenous Fenton reactions	158:202	heterogeneous and homogenous Fenton reactions	158:202	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	1	10	theme	diamine	400:406	arg1	EDTA					426:429	EDTA	426:429	EDTA	426:429	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	1	10	theme	diamine	400:406	arg1	acid					420:423	ethylene diamine tetraacetic acid	391:423	ethylene diamine tetraacetic acid (EDTA)	391:430	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	4	11	theme	Fe3O4	878:882	arg1	solubility					832:841	solubility	832:841	solubility	832:841	EDTA in β-cyclodextrin polymer can improve both the solubility and adsorption capacity to H2O2 of Fe3O4.
32088621	4	11	theme	Fe3O4	878:882	arg1	capacity					858:865	adsorption capacity	847:865	adsorption capacity	847:865	EDTA in β-cyclodextrin polymer can improve both the solubility and adsorption capacity to H2O2 of Fe3O4.
32088621	2	12	contain	has	479:481	arg2	performance					502:512	superior catalytic performance	483:512	superior catalytic performance for 4-nitrophenol and 2,4-dichlorophenol degradation	483:565	The 2,5-DBQ@ECDP-Fe3O4 has superior catalytic performance for 4-nitrophenol and 2,4-dichlorophenol degradation compared with control systems.
32088621	2	12	contain	has	479:481	arg1	ECDP-Fe3O4					468:477	The 2,5-DBQ@ECDP-Fe3O4	456:477	The 2,5-DBQ@ECDP-Fe3O4	456:477	The 2,5-DBQ@ECDP-Fe3O4 has superior catalytic performance for 4-nitrophenol and 2,4-dichlorophenol degradation compared with control systems.
32088621	6	13	theme	practical	1215:1223	arg1	applications					1225:1236	practical applications	1215:1236	practical applications	1215:1236	The current catalyst integrates the advantages of homogeneous and heterogeneous advanced oxidation processes and is promising in practical applications.
32088621	5	14	theme	2,5-DBQ	907:913	arg1	molecules					897:905	The quinone molecules 2,5-DBQ	885:913	The quinone molecules 2,5-DBQ in the β-cyclodextrin cavity	885:942	The quinone molecules 2,5-DBQ in the β-cyclodextrin cavity can accelerate Fe3+/Fe2+ cycle adjacent to the cavity, thus in favor of the decomposition of H2O2 into OH as main reactive oxidizing species.
32088621	1	15	theme	tetraacetic	408:418	arg1	EDTA					426:429	EDTA	426:429	EDTA	426:429	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	1	15	theme	tetraacetic	408:418	arg1	acid					420:423	ethylene diamine tetraacetic acid	391:423	ethylene diamine tetraacetic acid (EDTA)	391:430	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	4	16	dep	solubility	832:841	arg1	the					828:830	the	828:830	the	828:830	EDTA in β-cyclodextrin polymer can improve both the solubility and adsorption capacity to H2O2 of Fe3O4.
32088621	2	17	theme	@	467:467	arg1	ECDP-Fe3O4					468:477	The 2,5-DBQ@ECDP-Fe3O4	456:477	The 2,5-DBQ@ECDP-Fe3O4	456:477	The 2,5-DBQ@ECDP-Fe3O4 has superior catalytic performance for 4-nitrophenol and 2,4-dichlorophenol degradation compared with control systems.
32088621	4	18	from	EDTA	780:783	arg1	polymer					803:809	β-cyclodextrin polymer	788:809	β-cyclodextrin polymer	788:809	EDTA in β-cyclodextrin polymer can improve both the solubility and adsorption capacity to H2O2 of Fe3O4.
32088621	5	19	theme	oxidizing	1067:1075	arg1	species					1077:1083	main reactive oxidizing species	1053:1083	main reactive oxidizing species	1053:1083	The quinone molecules 2,5-DBQ in the β-cyclodextrin cavity can accelerate Fe3+/Fe2+ cycle adjacent to the cavity, thus in favor of the decomposition of H2O2 into OH as main reactive oxidizing species.
32088621	6	20	theme	current	1090:1096	arg1	promising					1202:1210	promising	1202:1210	promising	1202:1210	The current catalyst integrates the advantages of homogeneous and heterogeneous advanced oxidation processes and is promising in practical applications.
32088621	6	20	theme	current	1090:1096	arg1	catalyst					1098:1105	The current catalyst	1086:1105	The current catalyst	1086:1105	The current catalyst integrates the advantages of homogeneous and heterogeneous advanced oxidation processes and is promising in practical applications.
32088621	2	21	theme	2,5-DBQ	460:466	arg1	ECDP-Fe3O4					468:477	The 2,5-DBQ@ECDP-Fe3O4	456:477	The 2,5-DBQ@ECDP-Fe3O4	456:477	The 2,5-DBQ@ECDP-Fe3O4 has superior catalytic performance for 4-nitrophenol and 2,4-dichlorophenol degradation compared with control systems.
32088621	5	22	theme	H2O2	1037:1040	arg1	decomposition					1020:1032	the decomposition	1016:1032	the decomposition of H2O2 into OH as main reactive oxidizing species	1016:1083	The quinone molecules 2,5-DBQ in the β-cyclodextrin cavity can accelerate Fe3+/Fe2+ cycle adjacent to the cavity, thus in favor of the decomposition of H2O2 into OH as main reactive oxidizing species.
32088621	5	23	theme	reactive	1058:1065	arg1	species					1077:1083	main reactive oxidizing species	1053:1083	main reactive oxidizing species	1053:1083	The quinone molecules 2,5-DBQ in the β-cyclodextrin cavity can accelerate Fe3+/Fe2+ cycle adjacent to the cavity, thus in favor of the decomposition of H2O2 into OH as main reactive oxidizing species.
32088621	4	24	theme	β-cyclodextrin	788:801	arg1	polymer					803:809	β-cyclodextrin polymer	788:809	β-cyclodextrin polymer	788:809	EDTA in β-cyclodextrin polymer can improve both the solubility and adsorption capacity to H2O2 of Fe3O4.
32088621	0	25	theme	homogeneous	15:25	arg1	processes					64:72	homogeneous and heterogeneous advanced oxidation processes	15:72	homogeneous and heterogeneous advanced oxidation processes	15:72	Integration of homogeneous and heterogeneous advanced oxidation processes: Confined iron dancing with cyclodextrin polymer.
32088621	1	26	theme	Fe3O4	319:323	arg1	nanoparticles					325:337	Fe3O4 nanoparticles	319:337	Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA)	319:430	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	0	27	theme	advanced	45:52	arg1	processes					64:72	homogeneous and heterogeneous advanced oxidation processes	15:72	homogeneous and heterogeneous advanced oxidation processes	15:72	Integration of homogeneous and heterogeneous advanced oxidation processes: Confined iron dancing with cyclodextrin polymer.
32088621	1	28	theme	nanoparticles	325:337	arg1	ECDP-Fe3O4					292:301	ECDP-Fe3O4	292:301	ECDP-Fe3O4	292:301	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	1	28	theme	nanoparticles	325:337	arg1	composite					306:314	a composite	304:314	a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA)	304:430	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	1	28	theme	nanoparticles	325:337	arg1	nanoparticles					325:337	Fe3O4 nanoparticles	319:337	Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA)	319:430	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	6	29	theme	oxidation	1175:1183	arg1	processes					1185:1193	homogeneous and heterogeneous advanced oxidation processes	1136:1193	homogeneous and heterogeneous advanced oxidation processes	1136:1193	The current catalyst integrates the advantages of homogeneous and heterogeneous advanced oxidation processes and is promising in practical applications.
32088621	6	30	from	promising	1202:1210	arg1	applications					1225:1236	practical applications	1215:1236	practical applications	1215:1236	The current catalyst integrates the advantages of homogeneous and heterogeneous advanced oxidation processes and is promising in practical applications.
32088621	0	31	theme	heterogeneous	31:43	arg1	processes					64:72	homogeneous and heterogeneous advanced oxidation processes	15:72	homogeneous and heterogeneous advanced oxidation processes	15:72	Integration of homogeneous and heterogeneous advanced oxidation processes: Confined iron dancing with cyclodextrin polymer.
32088621	2	32	theme	catalytic	492:500	arg1	performance					502:512	superior catalytic performance	483:512	superior catalytic performance for 4-nitrophenol and 2,4-dichlorophenol degradation	483:565	The 2,5-DBQ@ECDP-Fe3O4 has superior catalytic performance for 4-nitrophenol and 2,4-dichlorophenol degradation compared with control systems.
32088621	5	33	theme	quinone	889:895	arg1	molecules					897:905	The quinone molecules 2,5-DBQ	885:913	The quinone molecules 2,5-DBQ in the β-cyclodextrin cavity	885:942	The quinone molecules 2,5-DBQ in the β-cyclodextrin cavity can accelerate Fe3+/Fe2+ cycle adjacent to the cavity, thus in favor of the decomposition of H2O2 into OH as main reactive oxidizing species.
32088621	0	34	theme	processes	64:72	arg1	Integration					0:10	Integration	0:10	Integration of homogeneous and heterogeneous advanced oxidation processes:	0:73	Integration of homogeneous and heterogeneous advanced oxidation processes: Confined iron dancing with cyclodextrin polymer.
32088621	2	35	theme	control	581:587	arg1	systems					589:595	control systems	581:595	control systems	581:595	The 2,5-DBQ@ECDP-Fe3O4 has superior catalytic performance for 4-nitrophenol and 2,4-dichlorophenol degradation compared with control systems.
32088621	2	36	dep	4-nitrophenol	518:530	arg1	degradation					555:565	degradation	555:565	degradation	555:565	The 2,5-DBQ@ECDP-Fe3O4 has superior catalytic performance for 4-nitrophenol and 2,4-dichlorophenol degradation compared with control systems.
32088621	3	37	theme	initial	641:647	arg1	site					656:659	the initial active site	637:659	the initial active site	637:659	Mechanism study revealed that although the initial active site is Fe3O4 loaded on ECDP, the actually catalyst is the iron ions released from Fe3O4 but confined within the composite.
32088621	3	37	theme	initial	641:647	arg1	Fe3O4					664:668	Fe3O4	664:668	Fe3O4 loaded on ECDP	664:683	Mechanism study revealed that although the initial active site is Fe3O4 loaded on ECDP, the actually catalyst is the iron ions released from Fe3O4 but confined within the composite.
32088621	5	38	theme	Fe3+/Fe2+	959:967	arg1	cycle					969:973	Fe3+/Fe2+ cycle	959:973	Fe3+/Fe2+ cycle adjacent to the cavity	959:996	The quinone molecules 2,5-DBQ in the β-cyclodextrin cavity can accelerate Fe3+/Fe2+ cycle adjacent to the cavity, thus in favor of the decomposition of H2O2 into OH as main reactive oxidizing species.
32088621	0	39	theme	oxidation	54:62	arg1	processes					64:72	homogeneous and heterogeneous advanced oxidation processes	15:72	homogeneous and heterogeneous advanced oxidation processes	15:72	Integration of homogeneous and heterogeneous advanced oxidation processes: Confined iron dancing with cyclodextrin polymer.
32088621	3	40	theme	iron	715:718	arg1	catalyst					699:706	the actually catalyst	686:706	the actually catalyst	686:706	Mechanism study revealed that although the initial active site is Fe3O4 loaded on ECDP, the actually catalyst is the iron ions released from Fe3O4 but confined within the composite.
32088621	3	40	theme	iron	715:718	arg1	ions					720:723	the iron ions	711:723	the iron ions released from Fe3O4 but confined within the composite	711:777	Mechanism study revealed that although the initial active site is Fe3O4 loaded on ECDP, the actually catalyst is the iron ions released from Fe3O4 but confined within the composite.
32088621	3	41	theme	active	649:654	arg1	site					656:659	the initial active site	637:659	the initial active site	637:659	Mechanism study revealed that although the initial active site is Fe3O4 loaded on ECDP, the actually catalyst is the iron ions released from Fe3O4 but confined within the composite.
32088621	3	41	theme	active	649:654	arg1	Fe3O4					664:668	Fe3O4	664:668	Fe3O4 loaded on ECDP	664:683	Mechanism study revealed that although the initial active site is Fe3O4 loaded on ECDP, the actually catalyst is the iron ions released from Fe3O4 but confined within the composite.
32088621	1	42	theme	novel	126:130	arg1	catalyst					132:139	A novel catalyst	124:139	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions	124:202	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	1	43	theme	β-cyclodextrin	356:369	arg1	ECDP					380:383	ECDP	380:383	ECDP	380:383	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	1	43	theme	β-cyclodextrin	356:369	arg1	polymer					371:377	a β-cyclodextrin polymer	354:377	a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA)	354:430	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	1	44	from	2,5-dihydroxy-1,4-benzoquinone	248:277	arg1	ECDP-Fe3O4					292:301	ECDP-Fe3O4	292:301	ECDP-Fe3O4	292:301	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	1	44	from	2,5-dihydroxy-1,4-benzoquinone	248:277	arg1	composite					306:314	a composite	304:314	a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA)	304:430	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	1	44	from	2,5-dihydroxy-1,4-benzoquinone	248:277	arg1	nanoparticles					325:337	Fe3O4 nanoparticles	319:337	Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA)	319:430	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	1	45	theme	cross-linking	435:447	arg1	agent					449:453	cross-linking agent	435:453	cross-linking agent	435:453	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
32088621	5	46	theme	adjacent	975:982	arg1	cycle					969:973	Fe3+/Fe2+ cycle	959:973	Fe3+/Fe2+ cycle adjacent to the cavity	959:996	The quinone molecules 2,5-DBQ in the β-cyclodextrin cavity can accelerate Fe3+/Fe2+ cycle adjacent to the cavity, thus in favor of the decomposition of H2O2 into OH as main reactive oxidizing species.
32088621	6	47	theme	heterogeneous	1152:1164	arg1	processes					1185:1193	homogeneous and heterogeneous advanced oxidation processes	1136:1193	homogeneous and heterogeneous advanced oxidation processes	1136:1193	The current catalyst integrates the advantages of homogeneous and heterogeneous advanced oxidation processes and is promising in practical applications.
32088621	6	48	theme	homogeneous	1136:1146	arg1	processes					1185:1193	homogeneous and heterogeneous advanced oxidation processes	1136:1193	homogeneous and heterogeneous advanced oxidation processes	1136:1193	The current catalyst integrates the advantages of homogeneous and heterogeneous advanced oxidation processes and is promising in practical applications.
32088621	6	49	theme	processes	1185:1193	arg1	advantages					1122:1131	the advantages	1118:1131	the advantages of homogeneous and heterogeneous advanced oxidation processes	1118:1193	The current catalyst integrates the advantages of homogeneous and heterogeneous advanced oxidation processes and is promising in practical applications.
32088621	6	50	theme	advanced	1166:1173	arg1	processes					1185:1193	homogeneous and heterogeneous advanced oxidation processes	1136:1193	homogeneous and heterogeneous advanced oxidation processes	1136:1193	The current catalyst integrates the advantages of homogeneous and heterogeneous advanced oxidation processes and is promising in practical applications.
32088621	4	51	theme	adsorption	847:856	arg1	capacity					858:865	adsorption capacity	847:865	adsorption capacity	847:865	EDTA in β-cyclodextrin polymer can improve both the solubility and adsorption capacity to H2O2 of Fe3O4.
32088621	5	52	theme	β-cyclodextrin	922:935	arg1	cavity					937:942	the β-cyclodextrin cavity	918:942	the β-cyclodextrin cavity	918:942	The quinone molecules 2,5-DBQ in the β-cyclodextrin cavity can accelerate Fe3+/Fe2+ cycle adjacent to the cavity, thus in favor of the decomposition of H2O2 into OH as main reactive oxidizing species.
32088621	2	53	theme	superior	483:490	arg1	performance					502:512	superior catalytic performance	483:512	superior catalytic performance for 4-nitrophenol and 2,4-dichlorophenol degradation	483:565	The 2,5-DBQ@ECDP-Fe3O4 has superior catalytic performance for 4-nitrophenol and 2,4-dichlorophenol degradation compared with control systems.
32088621	5	54	from	molecules	897:905	arg1	cavity					937:942	the β-cyclodextrin cavity	918:942	the β-cyclodextrin cavity	918:942	The quinone molecules 2,5-DBQ in the β-cyclodextrin cavity can accelerate Fe3+/Fe2+ cycle adjacent to the cavity, thus in favor of the decomposition of H2O2 into OH as main reactive oxidizing species.
32088621	1	55	theme	heterogeneous	158:170	arg1	reactions					194:202	heterogeneous and homogenous Fenton reactions	158:202	heterogeneous and homogenous Fenton reactions	158:202	A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe3O4, a composite of Fe3O4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent.
33074141	10	0	theme	pyruvyl	2002:2008	arg1	content					2010:2016	particularly the pyruvyl content	1985:2016	particularly the pyruvyl content	1985:2016	The results suggest that the composition of the water used in the bacteria's culture medium influenced the composition of XG, including the amount of Xanthan and particularly the pyruvyl content, and therefore needs to be considered when using this approach of injecting XG in oil fields as pyruvyl content affects viscosity.
33074141	9	1	theme	water	1641:1645	arg1	type					1633:1636	the type	1629:1636	the type of water used in the culture medium, where the XG were strongly reduced in the groups PW compared to DW while the pyruvyl content increased proportionally with the concentration of PW	1629:1820	ANOVA GLM applied to Raman peaks of interest (~1010 and ~ 1090 cm-1) and to PCA scores (Score 1 to Score 3) showed that both were influenced by the type of water used in the culture medium, where the XG were strongly reduced in the groups PW compared to DW while the pyruvyl content increased proportionally with the concentration of PW.
33074141	4	2	located	present	725:731	arg2	present					725:731	present	725:731	present	725:731	The production of XG may be affected by the composition of the PW, where the acetyl and pyruvyl radicals may be present in the mannoses.
33074141	4	2	located	present	725:731	arg2	radicals					709:716	the acetyl and pyruvyl radicals	686:716	the acetyl and pyruvyl radicals	686:716	The production of XG may be affected by the composition of the PW, where the acetyl and pyruvyl radicals may be present in the mannoses.
33074141	4	2	located	present	725:731	arg1	mannoses					740:747	the mannoses	736:747	the mannoses	736:747	The production of XG may be affected by the composition of the PW, where the acetyl and pyruvyl radicals may be present in the mannoses.
33074141	9	3	theme	culture	1659:1665	arg1	medium					1667:1672	the culture medium	1655:1672	the culture medium	1655:1672	ANOVA GLM applied to Raman peaks of interest (~1010 and ~ 1090 cm-1) and to PCA scores (Score 1 to Score 3) showed that both were influenced by the type of water used in the culture medium, where the XG were strongly reduced in the groups PW compared to DW while the pyruvyl content increased proportionally with the concentration of PW.
33074141	4	4	theme	XG	631:632	arg1	production					617:626	The production	613:626	The production of XG	613:632	The production of XG may be affected by the composition of the PW, where the acetyl and pyruvyl radicals may be present in the mannoses.
33074141	5	5	dep	produced	948:955	arg1	PW					958:959	PW	958:959	PW	958:959	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	2	6	theme	reservoir	267:275	arg1	formation					224:232	Geological formation	213:232	Geological formation	213:232	Geological formation and geographical location of the reservoir influence its physical, chemical and biological characteristics.
33074141	2	6	theme	reservoir	267:275	arg1	location					251:258	geographical location	238:258	geographical location	238:258	Geological formation and geographical location of the reservoir influence its physical, chemical and biological characteristics.
33074141	7	7	theme	25	1295:1296	arg1	%					1297:1297	%	1297:1297	%	1297:1297	Spectral features assigned to pyruvyl were seen in all samples mainly at ~1010 cm-1, with higher intensity when using DW and 25% PW.
33074141	0	8	from	field	105:109	arg1	water					77:81	produced water	68:81	produced water from a carbonated oil field through Raman spectroscopy	68:136	Composition of Xanthan gum produced by Xanthomonas campestris using produced water from a carbonated oil field through Raman spectroscopy.
33074141	5	9	theme	study	766:770	arg1	aim					754:756	The aim	750:756	The aim of this study	750:770	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	3	10	theme	salinity	566:573	arg1	function					554:561	function	554:561	function of salinity	554:573	Xanthan gum (XG), an exopolysaccharide (EPS) produced by Xanthomonas campestris, has been widely used in enhanced oil recovery (EOR) technology because of its high viscosity, pseudoplastic behavior, stability in function of salinity, temperature and alkaline conditions.
33074141	9	11	theme	interest	1521:1528	arg1	peaks					1512:1516	Raman peaks	1506:1516	Raman peaks of interest (~1010 and ~ 1090 cm-1)	1506:1552	ANOVA GLM applied to Raman peaks of interest (~1010 and ~ 1090 cm-1) and to PCA scores (Score 1 to Score 3) showed that both were influenced by the type of water used in the culture medium, where the XG were strongly reduced in the groups PW compared to DW while the pyruvyl content increased proportionally with the concentration of PW.
33074141	3	12	from	behavior	531:538	arg1	function					554:561	function	554:561	function of salinity	554:573	Xanthan gum (XG), an exopolysaccharide (EPS) produced by Xanthomonas campestris, has been widely used in enhanced oil recovery (EOR) technology because of its high viscosity, pseudoplastic behavior, stability in function of salinity, temperature and alkaline conditions.
33074141	6	13	theme	glucuronic	1152:1161	arg1	acid					1163:1166	glucuronic acid	1152:1166	glucuronic acid	1152:1166	The spectra of XG showed peaks referred to the main constituents of the Xanthan (glucose, mannose and glucuronic acid).
33074141	5	14	theme	produced	948:955	arg1	water					962:966	distilled (DW) or produced (PW) water	930:966	distilled (DW) or produced (PW) water	930:966	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	3	15	theme	alkaline	592:599	arg1	conditions					601:610	alkaline conditions	592:610	alkaline conditions	592:610	Xanthan gum (XG), an exopolysaccharide (EPS) produced by Xanthomonas campestris, has been widely used in enhanced oil recovery (EOR) technology because of its high viscosity, pseudoplastic behavior, stability in function of salinity, temperature and alkaline conditions.
33074141	5	16	from	composition	792:802	arg1	mediums					911:917	culture mediums	903:917	culture mediums containing distilled (DW) or produced (PW) water in different concentrations	903:994	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	3	17	theme	Xanthan	342:348	arg1	XG					355:356	XG	355:356	XG	355:356	Xanthan gum (XG), an exopolysaccharide (EPS) produced by Xanthomonas campestris, has been widely used in enhanced oil recovery (EOR) technology because of its high viscosity, pseudoplastic behavior, stability in function of salinity, temperature and alkaline conditions.
33074141	3	17	theme	Xanthan	342:348	arg1	gum					350:352	Xanthan gum	342:352	Xanthan gum (XG)	342:357	Xanthan gum (XG), an exopolysaccharide (EPS) produced by Xanthomonas campestris, has been widely used in enhanced oil recovery (EOR) technology because of its high viscosity, pseudoplastic behavior, stability in function of salinity, temperature and alkaline conditions.
33074141	3	17	theme	Xanthan	342:348	arg1	exopolysaccharide					363:379	an exopolysaccharide	360:379	an exopolysaccharide (EPS) produced by Xanthomonas campestris	360:420	Xanthan gum (XG), an exopolysaccharide (EPS) produced by Xanthomonas campestris, has been widely used in enhanced oil recovery (EOR) technology because of its high viscosity, pseudoplastic behavior, stability in function of salinity, temperature and alkaline conditions.
33074141	3	18	from	stability	541:549	arg1	function					554:561	function	554:561	function of salinity	554:573	Xanthan gum (XG), an exopolysaccharide (EPS) produced by Xanthomonas campestris, has been widely used in enhanced oil recovery (EOR) technology because of its high viscosity, pseudoplastic behavior, stability in function of salinity, temperature and alkaline conditions.
33074141	2	19	theme	geographical	238:249	arg1	location					251:258	geographical location	238:258	geographical location	238:258	Geological formation and geographical location of the reservoir influence its physical, chemical and biological characteristics.
33074141	10	20	theme	injecting	2084:2092	arg1	XG					2094:2095	injecting XG	2084:2095	injecting XG	2084:2095	The results suggest that the composition of the water used in the bacteria's culture medium influenced the composition of XG, including the amount of Xanthan and particularly the pyruvyl content, and therefore needs to be considered when using this approach of injecting XG in oil fields as pyruvyl content affects viscosity.
33074141	4	21	attach	present	725:731	arg2	present					725:731	present	725:731	present	725:731	The production of XG may be affected by the composition of the PW, where the acetyl and pyruvyl radicals may be present in the mannoses.
33074141	4	21	attach	present	725:731	arg2	radicals					709:716	the acetyl and pyruvyl radicals	686:716	the acetyl and pyruvyl radicals	686:716	The production of XG may be affected by the composition of the PW, where the acetyl and pyruvyl radicals may be present in the mannoses.
33074141	4	21	attach	present	725:731	arg1	mannoses					740:747	the mannoses	736:747	the mannoses	736:747	The production of XG may be affected by the composition of the PW, where the acetyl and pyruvyl radicals may be present in the mannoses.
33074141	5	22	theme	dispersive	1009:1018	arg1	1064 nm					1040:1046	1064 nm	1040:1046	1064 nm	1040:1046	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	5	22	theme	dispersive	1009:1018	arg1	spectroscopy					1026:1037	dispersive Raman spectroscopy	1009:1037	dispersive Raman spectroscopy (1064 nm)	1009:1047	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	10	23	theme	pyruvyl	2114:2120	arg1	content					2122:2128	pyruvyl content	2114:2128	pyruvyl content	2114:2128	The results suggest that the composition of the water used in the bacteria's culture medium influenced the composition of XG, including the amount of Xanthan and particularly the pyruvyl content, and therefore needs to be considered when using this approach of injecting XG in oil fields as pyruvyl content affects viscosity.
33074141	4	24	from	mannoses	740:747	arg1	radicals					709:716	the acetyl and pyruvyl radicals	686:716	the acetyl and pyruvyl radicals	686:716	The production of XG may be affected by the composition of the PW, where the acetyl and pyruvyl radicals may be present in the mannoses.
33074141	4	24	from	mannoses	740:747	arg1	present					725:731	present	725:731	present	725:731	The production of XG may be affected by the composition of the PW, where the acetyl and pyruvyl radicals may be present in the mannoses.
33074141	5	25	theme	different	971:979	arg1	concentrations					981:994	different concentrations	971:994	different concentrations	971:994	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	9	26	theme	pyruvyl	1752:1758	arg1	content					1760:1766	the pyruvyl content	1748:1766	the pyruvyl content	1748:1766	ANOVA GLM applied to Raman peaks of interest (~1010 and ~ 1090 cm-1) and to PCA scores (Score 1 to Score 3) showed that both were influenced by the type of water used in the culture medium, where the XG were strongly reduced in the groups PW compared to DW while the pyruvyl content increased proportionally with the concentration of PW.
33074141	3	27	from	temperature	576:586	arg1	function					554:561	function	554:561	function of salinity	554:573	Xanthan gum (XG), an exopolysaccharide (EPS) produced by Xanthomonas campestris, has been widely used in enhanced oil recovery (EOR) technology because of its high viscosity, pseudoplastic behavior, stability in function of salinity, temperature and alkaline conditions.
33074141	0	28	theme	oil	101:103	arg1	field					105:109	a carbonated oil field	88:109	a carbonated oil field through Raman spectroscopy	88:136	Composition of Xanthan gum produced by Xanthomonas campestris using produced water from a carbonated oil field through Raman spectroscopy.
33074141	3	29	used	used	439:442	arg2	XG					355:356	XG	355:356	XG	355:356	Xanthan gum (XG), an exopolysaccharide (EPS) produced by Xanthomonas campestris, has been widely used in enhanced oil recovery (EOR) technology because of its high viscosity, pseudoplastic behavior, stability in function of salinity, temperature and alkaline conditions.
33074141	3	29	used	used	439:442	arg2	gum					350:352	Xanthan gum	342:352	Xanthan gum (XG)	342:357	Xanthan gum (XG), an exopolysaccharide (EPS) produced by Xanthomonas campestris, has been widely used in enhanced oil recovery (EOR) technology because of its high viscosity, pseudoplastic behavior, stability in function of salinity, temperature and alkaline conditions.
33074141	3	29	used	used	439:442	arg2	exopolysaccharide					363:379	an exopolysaccharide	360:379	an exopolysaccharide (EPS) produced by Xanthomonas campestris	360:420	Xanthan gum (XG), an exopolysaccharide (EPS) produced by Xanthomonas campestris, has been widely used in enhanced oil recovery (EOR) technology because of its high viscosity, pseudoplastic behavior, stability in function of salinity, temperature and alkaline conditions.
33074141	0	30	theme	Xanthan	15:21	arg1	gum					23:25	Xanthan gum	15:25	Xanthan gum	15:25	Composition of Xanthan gum produced by Xanthomonas campestris using produced water from a carbonated oil field through Raman spectroscopy.
33074141	1	31	theme	Produced	139:146	arg1	water					148:152	Produced water	139:152	Produced water (PW)	139:157	Produced water (PW) is a by-product generated throughout oil exploration.
33074141	1	31	theme	Produced	139:146	arg1	by-product					164:173	a by-product	162:173	a by-product generated throughout oil exploration	162:210	Produced water (PW) is a by-product generated throughout oil exploration.
33074141	1	31	theme	Produced	139:146	arg1	PW					155:156	PW	155:156	PW	155:156	Produced water (PW) is a by-product generated throughout oil exploration.
33074141	5	32	theme	distilled	930:938	arg1	water					962:966	distilled (DW) or produced (PW) water	930:966	distilled (DW) or produced (PW) water	930:966	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	8	33	theme	pyruvate	1395:1402	arg1	presence					1376:1383	presence	1376:1383	presence of sodium pyruvate (~1040/~1050 and ~ 1432 cm-1)	1376:1432	PCA loadings showed that the peaks assigned to pyruvyl are consistent to presence of sodium pyruvate (~1040/~1050 and ~ 1432 cm-1) and were higher in the samples obtained in 25% PW.
33074141	9	34	theme	PW	1819:1820	arg1	concentration					1802:1814	the concentration	1798:1814	the concentration of PW	1798:1820	ANOVA GLM applied to Raman peaks of interest (~1010 and ~ 1090 cm-1) and to PCA scores (Score 1 to Score 3) showed that both were influenced by the type of water used in the culture medium, where the XG were strongly reduced in the groups PW compared to DW while the pyruvyl content increased proportionally with the concentration of PW.
33074141	0	35	theme	Xanthomonas	39:49	arg1	campestris					51:60	Xanthomonas campestris	39:60	Xanthomonas campestris using produced water from a carbonated oil field through Raman spectroscopy	39:136	Composition of Xanthan gum produced by Xanthomonas campestris using produced water from a carbonated oil field through Raman spectroscopy.
33074141	6	36	theme	Xanthan	1122:1128	arg1	constituents					1102:1113	the main constituents	1093:1113	the main constituents of the Xanthan (glucose, mannose and glucuronic acid)	1093:1167	The spectra of XG showed peaks referred to the main constituents of the Xanthan (glucose, mannose and glucuronic acid).
33074141	3	37	theme	oil	456:458	arg1	technology					475:484	enhanced oil recovery (EOR) technology	447:484	enhanced oil recovery (EOR) technology	447:484	Xanthan gum (XG), an exopolysaccharide (EPS) produced by Xanthomonas campestris, has been widely used in enhanced oil recovery (EOR) technology because of its high viscosity, pseudoplastic behavior, stability in function of salinity, temperature and alkaline conditions.
33074141	3	38	theme	high	501:504	arg1	viscosity					506:514	its high viscosity	497:514	its high viscosity	497:514	Xanthan gum (XG), an exopolysaccharide (EPS) produced by Xanthomonas campestris, has been widely used in enhanced oil recovery (EOR) technology because of its high viscosity, pseudoplastic behavior, stability in function of salinity, temperature and alkaline conditions.
33074141	8	39	theme	%	1479:1479	arg1	PW					1481:1482	25% PW	1477:1482	25% PW	1477:1482	PCA loadings showed that the peaks assigned to pyruvyl are consistent to presence of sodium pyruvate (~1040/~1050 and ~ 1432 cm-1) and were higher in the samples obtained in 25% PW.
33074141	6	40	dep	Xanthan	1122:1128	arg1	mannose					1140:1146	mannose	1140:1146	mannose	1140:1146	The spectra of XG showed peaks referred to the main constituents of the Xanthan (glucose, mannose and glucuronic acid).
33074141	6	40	dep	Xanthan	1122:1128	arg1	acid					1163:1166	glucuronic acid	1152:1166	glucuronic acid	1152:1166	The spectra of XG showed peaks referred to the main constituents of the Xanthan (glucose, mannose and glucuronic acid).
33074141	6	40	dep	Xanthan	1122:1128	arg1	glucose					1131:1137	glucose	1131:1137	glucose	1131:1137	The spectra of XG showed peaks referred to the main constituents of the Xanthan (glucose, mannose and glucuronic acid).
33074141	3	41	from	conditions	601:610	arg1	function					554:561	function	554:561	function of salinity	554:573	Xanthan gum (XG), an exopolysaccharide (EPS) produced by Xanthomonas campestris, has been widely used in enhanced oil recovery (EOR) technology because of its high viscosity, pseudoplastic behavior, stability in function of salinity, temperature and alkaline conditions.
33074141	2	42	theme	physical	291:298	arg1	characteristics					325:339	its physical, chemical and biological characteristics	287:339	its physical, chemical and biological characteristics	287:339	Geological formation and geographical location of the reservoir influence its physical, chemical and biological characteristics.
33074141	7	43	theme	%	1297:1297	arg1	PW					1299:1300	25% PW	1295:1300	25% PW	1295:1300	Spectral features assigned to pyruvyl were seen in all samples mainly at ~1010 cm-1, with higher intensity when using DW and 25% PW.
33074141	9	44	theme	ANOVA	1485:1489	arg1	GLM					1491:1493	ANOVA GLM	1485:1493	ANOVA GLM applied to Raman peaks of interest (~1010 and ~ 1090 cm-1) and to PCA scores (Score 1 to Score 3)	1485:1591	ANOVA GLM applied to Raman peaks of interest (~1010 and ~ 1090 cm-1) and to PCA scores (Score 1 to Score 3) showed that both were influenced by the type of water used in the culture medium, where the XG were strongly reduced in the groups PW compared to DW while the pyruvyl content increased proportionally with the concentration of PW.
33074141	2	45	theme	biological	314:323	arg1	characteristics					325:339	its physical, chemical and biological characteristics	287:339	its physical, chemical and biological characteristics	287:339	Geological formation and geographical location of the reservoir influence its physical, chemical and biological characteristics.
33074141	9	46	dep	reduced	1702:1708	arg1	compared					1727:1734	compared	1727:1734	compared to DW while the pyruvyl content increased proportionally with the concentration of PW	1727:1820	ANOVA GLM applied to Raman peaks of interest (~1010 and ~ 1090 cm-1) and to PCA scores (Score 1 to Score 3) showed that both were influenced by the type of water used in the culture medium, where the XG were strongly reduced in the groups PW compared to DW while the pyruvyl content increased proportionally with the concentration of PW.
33074141	7	47	theme	higher	1260:1265	arg1	intensity					1267:1275	higher intensity	1260:1275	higher intensity	1260:1275	Spectral features assigned to pyruvyl were seen in all samples mainly at ~1010 cm-1, with higher intensity when using DW and 25% PW.
33074141	5	48	dep	distilled	930:938	arg1	DW					941:942	DW	941:942	DW	941:942	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	0	49	theme	Raman	119:123	arg1	spectroscopy					125:136	Raman spectroscopy	119:136	Raman spectroscopy	119:136	Composition of Xanthan gum produced by Xanthomonas campestris using produced water from a carbonated oil field through Raman spectroscopy.
33074141	2	50	theme	chemical	301:308	arg1	characteristics					325:339	its physical, chemical and biological characteristics	287:339	its physical, chemical and biological characteristics	287:339	Geological formation and geographical location of the reservoir influence its physical, chemical and biological characteristics.
33074141	9	51	theme	Raman	1506:1510	arg1	peaks					1512:1516	Raman peaks	1506:1516	Raman peaks of interest (~1010 and ~ 1090 cm-1)	1506:1552	ANOVA GLM applied to Raman peaks of interest (~1010 and ~ 1090 cm-1) and to PCA scores (Score 1 to Score 3) showed that both were influenced by the type of water used in the culture medium, where the XG were strongly reduced in the groups PW compared to DW while the pyruvyl content increased proportionally with the concentration of PW.
33074141	10	52	theme	water	1871:1875	arg1	composition					1852:1862	the composition	1848:1862	the composition of the water used in the bacteria's culture medium	1848:1913	The results suggest that the composition of the water used in the bacteria's culture medium influenced the composition of XG, including the amount of Xanthan and particularly the pyruvyl content, and therefore needs to be considered when using this approach of injecting XG in oil fields as pyruvyl content affects viscosity.
33074141	1	53	theme	oil	196:198	arg1	exploration					200:210	oil exploration	196:210	oil exploration	196:210	Produced water (PW) is a by-product generated throughout oil exploration.
33074141	6	54	theme	main	1097:1100	arg1	constituents					1102:1113	the main constituents	1093:1113	the main constituents of the Xanthan (glucose, mannose and glucuronic acid)	1093:1167	The spectra of XG showed peaks referred to the main constituents of the Xanthan (glucose, mannose and glucuronic acid).
33074141	10	55	theme	culture	1900:1906	arg1	medium					1908:1913	the bacteria's culture medium	1885:1913	the bacteria's culture medium	1885:1913	The results suggest that the composition of the water used in the bacteria's culture medium influenced the composition of XG, including the amount of Xanthan and particularly the pyruvyl content, and therefore needs to be considered when using this approach of injecting XG in oil fields as pyruvyl content affects viscosity.
33074141	9	56	dep	Score	1573:1577	arg1	to					1581:1582	to	1581:1582	to	1581:1582	ANOVA GLM applied to Raman peaks of interest (~1010 and ~ 1090 cm-1) and to PCA scores (Score 1 to Score 3) showed that both were influenced by the type of water used in the culture medium, where the XG were strongly reduced in the groups PW compared to DW while the pyruvyl content increased proportionally with the concentration of PW.
33074141	9	56	dep	Score	1573:1577	arg1	Score					1584:1588	Score 3	1584:1590	Score 1 to Score 3	1573:1590	ANOVA GLM applied to Raman peaks of interest (~1010 and ~ 1090 cm-1) and to PCA scores (Score 1 to Score 3) showed that both were influenced by the type of water used in the culture medium, where the XG were strongly reduced in the groups PW compared to DW while the pyruvyl content increased proportionally with the concentration of PW.
33074141	2	57	theme	Geological	213:222	arg1	formation					224:232	Geological formation	213:232	Geological formation	213:232	Geological formation and geographical location of the reservoir influence its physical, chemical and biological characteristics.
33074141	8	58	theme	PCA	1303:1305	arg1	loadings					1307:1314	PCA loadings	1303:1314	PCA loadings	1303:1314	PCA loadings showed that the peaks assigned to pyruvyl are consistent to presence of sodium pyruvate (~1040/~1050 and ~ 1432 cm-1) and were higher in the samples obtained in 25% PW.
33074141	3	59	theme	pseudoplastic	517:529	arg1	behavior					531:538	pseudoplastic behavior	517:538	pseudoplastic behavior	517:538	Xanthan gum (XG), an exopolysaccharide (EPS) produced by Xanthomonas campestris, has been widely used in enhanced oil recovery (EOR) technology because of its high viscosity, pseudoplastic behavior, stability in function of salinity, temperature and alkaline conditions.
33074141	4	60	theme	PW	676:677	arg1	composition					657:667	the composition	653:667	the composition	653:667	The production of XG may be affected by the composition of the PW, where the acetyl and pyruvyl radicals may be present in the mannoses.
33074141	0	61	theme	produced	68:75	arg1	water					77:81	produced water	68:81	produced water from a carbonated oil field through Raman spectroscopy	68:136	Composition of Xanthan gum produced by Xanthomonas campestris using produced water from a carbonated oil field through Raman spectroscopy.
33074141	5	62	theme	XG	807:808	arg1	composition					792:802	the composition	788:802	the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations	788:994	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	10	63	theme	XG	2094:2095	arg1	approach					2072:2079	this approach	2067:2079	this approach of injecting XG	2067:2095	The results suggest that the composition of the water used in the bacteria's culture medium influenced the composition of XG, including the amount of Xanthan and particularly the pyruvyl content, and therefore needs to be considered when using this approach of injecting XG in oil fields as pyruvyl content affects viscosity.
33074141	6	64	theme	XG	1065:1066	arg1	spectra					1054:1060	The spectra	1050:1060	The spectra of XG	1050:1066	The spectra of XG showed peaks referred to the main constituents of the Xanthan (glucose, mannose and glucuronic acid).
33074141	4	65	from	present	725:731	arg1	mannoses					740:747	the mannoses	736:747	the mannoses	736:747	The production of XG may be affected by the composition of the PW, where the acetyl and pyruvyl radicals may be present in the mannoses.
33074141	9	66	theme	PCA	1561:1563	arg1	Score					1573:1577	Score 1 to Score 3	1573:1590	Score 1 to Score 3	1573:1590	ANOVA GLM applied to Raman peaks of interest (~1010 and ~ 1090 cm-1) and to PCA scores (Score 1 to Score 3) showed that both were influenced by the type of water used in the culture medium, where the XG were strongly reduced in the groups PW compared to DW while the pyruvyl content increased proportionally with the concentration of PW.
33074141	9	66	theme	PCA	1561:1563	arg1	scores					1565:1570	PCA scores	1561:1570	PCA scores (Score 1 to Score 3)	1561:1591	ANOVA GLM applied to Raman peaks of interest (~1010 and ~ 1090 cm-1) and to PCA scores (Score 1 to Score 3) showed that both were influenced by the type of water used in the culture medium, where the XG were strongly reduced in the groups PW compared to DW while the pyruvyl content increased proportionally with the concentration of PW.
33074141	3	67	from	viscosity	506:514	arg1	function					554:561	function	554:561	function of salinity	554:573	Xanthan gum (XG), an exopolysaccharide (EPS) produced by Xanthomonas campestris, has been widely used in enhanced oil recovery (EOR) technology because of its high viscosity, pseudoplastic behavior, stability in function of salinity, temperature and alkaline conditions.
33074141	0	68	theme	carbonated	90:99	arg1	field					105:109	a carbonated oil field	88:109	a carbonated oil field through Raman spectroscopy	88:136	Composition of Xanthan gum produced by Xanthomonas campestris using produced water from a carbonated oil field through Raman spectroscopy.
33074141	5	69	theme	Raman	1020:1024	arg1	1064 nm					1040:1046	1064 nm	1040:1046	1064 nm	1040:1046	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	5	69	theme	Raman	1020:1024	arg1	spectroscopy					1026:1037	dispersive Raman spectroscopy	1009:1037	dispersive Raman spectroscopy (1064 nm)	1009:1047	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	5	70	theme	acetyl	873:878	arg1	XG					807:808	XG	807:808	XG produced by X. campestris	807:834	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	5	70	theme	acetyl	873:878	arg1	groups					892:897	acetyl and pyruvyl groups	873:897	groups	892:897	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	10	71	theme	XG	1945:1946	arg1	composition					1930:1940	the composition	1926:1940	the composition of XG, including the amount of Xanthan and particularly the pyruvyl content	1926:2016	The results suggest that the composition of the water used in the bacteria's culture medium influenced the composition of XG, including the amount of Xanthan and particularly the pyruvyl content, and therefore needs to be considered when using this approach of injecting XG in oil fields as pyruvyl content affects viscosity.
33074141	4	72	theme	pyruvyl	701:707	arg1	radicals					709:716	the acetyl and pyruvyl radicals	686:716	the acetyl and pyruvyl radicals	686:716	The production of XG may be affected by the composition of the PW, where the acetyl and pyruvyl radicals may be present in the mannoses.
33074141	4	72	theme	pyruvyl	701:707	arg1	present					725:731	present	725:731	present	725:731	The production of XG may be affected by the composition of the PW, where the acetyl and pyruvyl radicals may be present in the mannoses.
33074141	5	73	theme	pyruvyl	884:890	arg1	XG					807:808	XG	807:808	XG produced by X. campestris	807:834	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	5	73	theme	pyruvyl	884:890	arg1	groups					892:897	acetyl and pyruvyl groups	873:897	groups	892:897	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	10	74	theme	oil	2100:2102	arg1	fields					2104:2109	oil fields	2100:2109	oil fields	2100:2109	The results suggest that the composition of the water used in the bacteria's culture medium influenced the composition of XG, including the amount of Xanthan and particularly the pyruvyl content, and therefore needs to be considered when using this approach of injecting XG in oil fields as pyruvyl content affects viscosity.
33074141	10	75	theme	Xanthan	1973:1979	arg1	amount					1963:1968	the amount	1959:1968	the amount of Xanthan	1959:1979	The results suggest that the composition of the water used in the bacteria's culture medium influenced the composition of XG, including the amount of Xanthan and particularly the pyruvyl content, and therefore needs to be considered when using this approach of injecting XG in oil fields as pyruvyl content affects viscosity.
33074141	10	75	theme	Xanthan	1973:1979	arg1	content					2010:2016	particularly the pyruvyl content	1985:2016	particularly the pyruvyl content	1985:2016	The results suggest that the composition of the water used in the bacteria's culture medium influenced the composition of XG, including the amount of Xanthan and particularly the pyruvyl content, and therefore needs to be considered when using this approach of injecting XG in oil fields as pyruvyl content affects viscosity.
33074141	10	75	theme	Xanthan	1973:1979	arg1	Xanthan					1973:1979	Xanthan	1973:1979	Xanthan	1973:1979	The results suggest that the composition of the water used in the bacteria's culture medium influenced the composition of XG, including the amount of Xanthan and particularly the pyruvyl content, and therefore needs to be considered when using this approach of injecting XG in oil fields as pyruvyl content affects viscosity.
33074141	7	76	theme	Spectral	1170:1177	arg1	features					1179:1186	Spectral features	1170:1186	Spectral features assigned to pyruvyl	1170:1206	Spectral features assigned to pyruvyl were seen in all samples mainly at ~1010 cm-1, with higher intensity when using DW and 25% PW.
33074141	5	77	dep	X.	822:823	arg1	campestris					825:834	X. campestris	822:834	X. campestris	822:834	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	0	78	theme	gum	23:25	arg1	Composition					0:10	Composition	0:10	Composition of Xanthan gum	0:25	Composition of Xanthan gum produced by Xanthomonas campestris using produced water from a carbonated oil field through Raman spectroscopy.
33074141	5	79	theme	Xanthan	864:870	arg1	Xanthan					864:870	Xanthan	864:870	Xanthan	864:870	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	5	79	theme	Xanthan	864:870	arg1	amount					854:859	the amount	850:859	the amount of Xanthan	850:870	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	5	79	theme	Xanthan	864:870	arg1	XG					807:808	XG	807:808	XG produced by X. campestris	807:834	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	8	80	theme	sodium	1388:1393	arg1	pyruvate					1395:1402	sodium pyruvate	1388:1402	sodium pyruvate (~1040/~1050 and ~ 1432 cm-1)	1388:1432	PCA loadings showed that the peaks assigned to pyruvyl are consistent to presence of sodium pyruvate (~1040/~1050 and ~ 1432 cm-1) and were higher in the samples obtained in 25% PW.
33074141	8	80	theme	sodium	1388:1393	arg1	and ~ 1432 cm-1					1417:1431	~1040/~1050 and ~ 1432 cm-1	1405:1431	~1040/~1050 and ~ 1432 cm-1	1405:1431	PCA loadings showed that the peaks assigned to pyruvyl are consistent to presence of sodium pyruvate (~1040/~1050 and ~ 1432 cm-1) and were higher in the samples obtained in 25% PW.
33074141	4	81	theme	acetyl	690:695	arg1	radicals					709:716	the acetyl and pyruvyl radicals	686:716	the acetyl and pyruvyl radicals	686:716	The production of XG may be affected by the composition of the PW, where the acetyl and pyruvyl radicals may be present in the mannoses.
33074141	4	81	theme	acetyl	690:695	arg1	present					725:731	present	725:731	present	725:731	The production of XG may be affected by the composition of the PW, where the acetyl and pyruvyl radicals may be present in the mannoses.
33074141	3	82	theme	enhanced	447:454	arg1	technology					475:484	enhanced oil recovery (EOR) technology	447:484	enhanced oil recovery (EOR) technology	447:484	Xanthan gum (XG), an exopolysaccharide (EPS) produced by Xanthomonas campestris, has been widely used in enhanced oil recovery (EOR) technology because of its high viscosity, pseudoplastic behavior, stability in function of salinity, temperature and alkaline conditions.
33074141	3	83	theme	Xanthomonas	399:409	arg1	campestris					411:420	Xanthomonas campestris	399:420	Xanthomonas campestris	399:420	Xanthan gum (XG), an exopolysaccharide (EPS) produced by Xanthomonas campestris, has been widely used in enhanced oil recovery (EOR) technology because of its high viscosity, pseudoplastic behavior, stability in function of salinity, temperature and alkaline conditions.
33074141	3	84	theme	EOR	470:472	arg1	technology					475:484	enhanced oil recovery (EOR) technology	447:484	enhanced oil recovery (EOR) technology	447:484	Xanthan gum (XG), an exopolysaccharide (EPS) produced by Xanthomonas campestris, has been widely used in enhanced oil recovery (EOR) technology because of its high viscosity, pseudoplastic behavior, stability in function of salinity, temperature and alkaline conditions.
33074141	8	85	theme	25	1477:1478	arg1	%					1479:1479	%	1479:1479	%	1479:1479	PCA loadings showed that the peaks assigned to pyruvyl are consistent to presence of sodium pyruvate (~1040/~1050 and ~ 1432 cm-1) and were higher in the samples obtained in 25% PW.
33074141	3	86	theme	recovery	460:467	arg1	technology					475:484	enhanced oil recovery (EOR) technology	447:484	enhanced oil recovery (EOR) technology	447:484	Xanthan gum (XG), an exopolysaccharide (EPS) produced by Xanthomonas campestris, has been widely used in enhanced oil recovery (EOR) technology because of its high viscosity, pseudoplastic behavior, stability in function of salinity, temperature and alkaline conditions.
33074141	5	87	theme	culture	903:909	arg1	mediums					911:917	culture mediums	903:917	culture mediums containing distilled (DW) or produced (PW) water in different concentrations	903:994	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	5	88	contain	containing	919:928	arg2	water					962:966	distilled (DW) or produced (PW) water	930:966	distilled (DW) or produced (PW) water	930:966	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
33074141	5	88	contain	containing	919:928	arg1	mediums					911:917	culture mediums	903:917	culture mediums containing distilled (DW) or produced (PW) water in different concentrations	903:994	The aim of this study was to evaluate the composition of XG produced by X. campestris, particularly the amount of Xanthan, acetyl and pyruvyl groups, in culture mediums containing distilled (DW) or produced (PW) water in different concentrations, by means of dispersive Raman spectroscopy (1064 nm).
35574788	1	0	theme	nanocrystals	172:183	arg1	phase					156:160	the cholesteric phase	140:160	the cholesteric phase of chitin nanocrystals and N-isopropylacrylamide	140:209	Anisotropic stimuli-responsive microgels based upon the cholesteric phase of chitin nanocrystals and N-isopropylacrylamide were designed and synthesized.
35574788	4	1	theme	imprinted	603:611	arg1	phase					625:629	the imprinted cholesteric phase	599:629	the imprinted cholesteric phase	599:629	As designed, the imprinted cholesteric phase induced an asymmetric response to temperature, leading to a change in shape and optical properties.
35574788	0	2	theme	Nanocrystals	74:85	arg1	Phase					58:62	the Cholesteric Phase	42:62	the Cholesteric Phase of Chitin Nanocrystals	42:85	Anisotropic Responsive Microgels Based on the Cholesteric Phase of Chitin Nanocrystals.
35574788	6	3	theme	polymer	949:955	arg1	matrix					957:962	a polymer matrix	947:962	a polymer matrix	947:962	Access to synthetic structures having a self-assembled twisted texture derived from cholesterics embedded within a polymer matrix will provide guidelines for designing biopolymer composites with programmable motion.
35574788	4	4	theme	optical	711:717	arg1	properties					719:728	optical properties	711:728	optical properties	711:728	As designed, the imprinted cholesteric phase induced an asymmetric response to temperature, leading to a change in shape and optical properties.
35574788	5	5	theme	cholesteric	748:758	arg1	able					773:776	able	773:776	able	773:776	Furthermore, the cholesteric structure is able to deform, facilitating transport into a small channel.
35574788	5	5	theme	cholesteric	748:758	arg1	structure					760:768	the cholesteric structure	744:768	the cholesteric structure	744:768	Furthermore, the cholesteric structure is able to deform, facilitating transport into a small channel.
35574788	3	6	theme	concentric-packing	556:573	arg1	structure					575:583	a concentric-packing structure	554:583	a concentric-packing structure	554:583	Changes in the microgel volume led to changes in the texture, where microgels comprising up to six bands exhibited a twisted bipolar texture, while those with greater volumes displayed a concentric-packing structure.
35574788	6	7	contain	having	865:870	arg2	texture					897:903	a self-assembled twisted texture	872:903	a self-assembled twisted texture derived from cholesterics embedded within a polymer matrix	872:962	Access to synthetic structures having a self-assembled twisted texture derived from cholesterics embedded within a polymer matrix will provide guidelines for designing biopolymer composites with programmable motion.
35574788	6	7	contain	having	865:870	arg1	structures					854:863	synthetic structures	844:863	synthetic structures having a self-assembled twisted texture derived from cholesterics embedded within a polymer matrix	844:962	Access to synthetic structures having a self-assembled twisted texture derived from cholesterics embedded within a polymer matrix will provide guidelines for designing biopolymer composites with programmable motion.
35574788	4	8	theme	cholesteric	613:623	arg1	phase					625:629	the imprinted cholesteric phase	599:629	the imprinted cholesteric phase	599:629	As designed, the imprinted cholesteric phase induced an asymmetric response to temperature, leading to a change in shape and optical properties.
35574788	3	9	from	changes	407:413	arg1	texture					422:428	the texture	418:428	the texture	418:428	Changes in the microgel volume led to changes in the texture, where microgels comprising up to six bands exhibited a twisted bipolar texture, while those with greater volumes displayed a concentric-packing structure.
35574788	4	10	from	change	691:696	arg1	shape					701:705	shape	701:705	shape	701:705	As designed, the imprinted cholesteric phase induced an asymmetric response to temperature, leading to a change in shape and optical properties.
35574788	4	10	from	change	691:696	arg1	properties					719:728	optical properties	711:728	optical properties	711:728	As designed, the imprinted cholesteric phase induced an asymmetric response to temperature, leading to a change in shape and optical properties.
35574788	1	11	theme	N-isopropylacrylamide	189:209	arg1	phase					156:160	the cholesteric phase	140:160	the cholesteric phase of chitin nanocrystals and N-isopropylacrylamide	140:209	Anisotropic stimuli-responsive microgels based upon the cholesteric phase of chitin nanocrystals and N-isopropylacrylamide were designed and synthesized.
35574788	3	12	theme	microgel	384:391	arg1	volume					393:398	the microgel volume	380:398	the microgel volume	380:398	Changes in the microgel volume led to changes in the texture, where microgels comprising up to six bands exhibited a twisted bipolar texture, while those with greater volumes displayed a concentric-packing structure.
35574788	0	13	theme	Responsive	12:21	arg1	Microgels					23:31	Anisotropic Responsive Microgels	0:31	Anisotropic Responsive Microgels	0:31	Anisotropic Responsive Microgels Based on the Cholesteric Phase of Chitin Nanocrystals.
35574788	1	14	theme	Anisotropic	88:98	arg1	microgels					119:127	Anisotropic stimuli-responsive microgels	88:127	Anisotropic stimuli-responsive microgels based upon the cholesteric phase of chitin nanocrystals and N-isopropylacrylamide	88:209	Anisotropic stimuli-responsive microgels based upon the cholesteric phase of chitin nanocrystals and N-isopropylacrylamide were designed and synthesized.
35574788	6	15	theme	embedded	931:938	arg1	cholesterics					918:929	cholesterics	918:929	cholesterics embedded within a polymer matrix	918:962	Access to synthetic structures having a self-assembled twisted texture derived from cholesterics embedded within a polymer matrix will provide guidelines for designing biopolymer composites with programmable motion.
35574788	0	16	theme	Anisotropic	0:10	arg1	Microgels					23:31	Anisotropic Responsive Microgels	0:31	Anisotropic Responsive Microgels	0:31	Anisotropic Responsive Microgels Based on the Cholesteric Phase of Chitin Nanocrystals.
35574788	6	17	theme	programmable	1029:1040	arg1	motion					1042:1047	programmable motion	1029:1047	programmable motion	1029:1047	Access to synthetic structures having a self-assembled twisted texture derived from cholesterics embedded within a polymer matrix will provide guidelines for designing biopolymer composites with programmable motion.
35574788	1	18	theme	stimuli-responsive	100:117	arg1	microgels					119:127	Anisotropic stimuli-responsive microgels	88:127	Anisotropic stimuli-responsive microgels based upon the cholesteric phase of chitin nanocrystals and N-isopropylacrylamide	88:209	Anisotropic stimuli-responsive microgels based upon the cholesteric phase of chitin nanocrystals and N-isopropylacrylamide were designed and synthesized.
35574788	3	19	theme	twisted	486:492	arg1	texture					502:508	a twisted bipolar texture	484:508	a twisted bipolar texture	484:508	Changes in the microgel volume led to changes in the texture, where microgels comprising up to six bands exhibited a twisted bipolar texture, while those with greater volumes displayed a concentric-packing structure.
35574788	6	20	theme	biopolymer	1002:1011	arg1	composites					1013:1022	biopolymer composites	1002:1022	biopolymer composites with programmable motion	1002:1047	Access to synthetic structures having a self-assembled twisted texture derived from cholesterics embedded within a polymer matrix will provide guidelines for designing biopolymer composites with programmable motion.
35574788	6	21	theme	twisted	889:895	arg1	texture					897:903	a self-assembled twisted texture	872:903	a self-assembled twisted texture derived from cholesterics embedded within a polymer matrix	872:962	Access to synthetic structures having a self-assembled twisted texture derived from cholesterics embedded within a polymer matrix will provide guidelines for designing biopolymer composites with programmable motion.
35574788	5	22	theme	small	819:823	arg1	channel					825:831	a small channel	817:831	a small channel	817:831	Furthermore, the cholesteric structure is able to deform, facilitating transport into a small channel.
35574788	6	23	theme	self-assembled	874:887	arg1	texture					897:903	a self-assembled twisted texture	872:903	a self-assembled twisted texture derived from cholesterics embedded within a polymer matrix	872:962	Access to synthetic structures having a self-assembled twisted texture derived from cholesterics embedded within a polymer matrix will provide guidelines for designing biopolymer composites with programmable motion.
35574788	2	24	dep	microgel	338:345	arg1	anisotropy					357:366	anisotropy	357:366	anisotropy	357:366	The cholesteric structure was interrogated, and the texture was shown to directly influence the microgel shape and anisotropy.
35574788	2	24	dep	microgel	338:345	arg1	shape					347:351	shape	347:351	shape	347:351	The cholesteric structure was interrogated, and the texture was shown to directly influence the microgel shape and anisotropy.
35574788	0	25	theme	Cholesteric	46:56	arg1	Phase					58:62	the Cholesteric Phase	42:62	the Cholesteric Phase of Chitin Nanocrystals	42:85	Anisotropic Responsive Microgels Based on the Cholesteric Phase of Chitin Nanocrystals.
35574788	2	26	theme	cholesteric	246:256	arg1	structure					258:266	The cholesteric structure	242:266	The cholesteric structure	242:266	The cholesteric structure was interrogated, and the texture was shown to directly influence the microgel shape and anisotropy.
35574788	3	27	dep	six	464:466	arg1	to					461:462	to	461:462	to	461:462	Changes in the microgel volume led to changes in the texture, where microgels comprising up to six bands exhibited a twisted bipolar texture, while those with greater volumes displayed a concentric-packing structure.
35574788	1	28	theme	cholesteric	144:154	arg1	phase					156:160	the cholesteric phase	140:160	the cholesteric phase of chitin nanocrystals and N-isopropylacrylamide	140:209	Anisotropic stimuli-responsive microgels based upon the cholesteric phase of chitin nanocrystals and N-isopropylacrylamide were designed and synthesized.
35574788	3	29	from	Changes	369:375	arg1	volume					393:398	the microgel volume	380:398	the microgel volume	380:398	Changes in the microgel volume led to changes in the texture, where microgels comprising up to six bands exhibited a twisted bipolar texture, while those with greater volumes displayed a concentric-packing structure.
35574788	6	30	with	composites	1013:1022	arg1	motion					1042:1047	programmable motion	1029:1047	programmable motion	1029:1047	Access to synthetic structures having a self-assembled twisted texture derived from cholesterics embedded within a polymer matrix will provide guidelines for designing biopolymer composites with programmable motion.
35574788	6	31	attach	derived	905:911	arg2	texture					897:903	a self-assembled twisted texture	872:903	a self-assembled twisted texture derived from cholesterics embedded within a polymer matrix	872:962	Access to synthetic structures having a self-assembled twisted texture derived from cholesterics embedded within a polymer matrix will provide guidelines for designing biopolymer composites with programmable motion.
35574788	6	31	attach	derived	905:911	arg1	cholesterics					918:929	cholesterics	918:929	cholesterics embedded within a polymer matrix	918:962	Access to synthetic structures having a self-assembled twisted texture derived from cholesterics embedded within a polymer matrix will provide guidelines for designing biopolymer composites with programmable motion.
35574788	3	32	theme	bipolar	494:500	arg1	texture					502:508	a twisted bipolar texture	484:508	a twisted bipolar texture	484:508	Changes in the microgel volume led to changes in the texture, where microgels comprising up to six bands exhibited a twisted bipolar texture, while those with greater volumes displayed a concentric-packing structure.
35574788	0	33	theme	Chitin	67:72	arg1	Nanocrystals					74:85	Chitin Nanocrystals	67:85	Chitin Nanocrystals	67:85	Anisotropic Responsive Microgels Based on the Cholesteric Phase of Chitin Nanocrystals.
35574788	6	34	theme	synthetic	844:852	arg1	structures					854:863	synthetic structures	844:863	synthetic structures having a self-assembled twisted texture derived from cholesterics embedded within a polymer matrix	844:962	Access to synthetic structures having a self-assembled twisted texture derived from cholesterics embedded within a polymer matrix will provide guidelines for designing biopolymer composites with programmable motion.
35574788	3	35	theme	greater	528:534	arg1	volumes					536:542	greater volumes	528:542	greater volumes	528:542	Changes in the microgel volume led to changes in the texture, where microgels comprising up to six bands exhibited a twisted bipolar texture, while those with greater volumes displayed a concentric-packing structure.
35574788	1	36	theme	chitin	165:170	arg1	nanocrystals					172:183	chitin nanocrystals	165:183	chitin nanocrystals	165:183	Anisotropic stimuli-responsive microgels based upon the cholesteric phase of chitin nanocrystals and N-isopropylacrylamide were designed and synthesized.
35574788	4	37	theme	asymmetric	642:651	arg1	response					653:660	an asymmetric response	639:660	an asymmetric response to temperature	639:675	As designed, the imprinted cholesteric phase induced an asymmetric response to temperature, leading to a change in shape and optical properties.
32829100	0	0	theme	release	77:83	arg1	properties					85:94	the mechanical, cross-linking, and release properties	42:94	properties	85:94	Effects of microencapsulated abamectin on the mechanical, cross-linking, and release properties of PBS.
32829100	6	1	from	break	900:904	arg1	elongation					882:891	the elongation	878:891	the elongation at the break of composite films	878:923	With an increase in the A-G-G microcapsule content, the elongation at the break of composite films increases significantly.
32829100	5	2	theme	microcapsules	811:823	arg1	PBS					782:784	PBS	782:784	PBS	782:784	Results show that there is a strong interaction between the CO groups in PBS and free OH of the A-G-G microcapsules.
32829100	5	2	theme	microcapsules	811:823	arg1	OH					795:796	free OH	790:796	free OH of the A-G-G microcapsules	790:823	Results show that there is a strong interaction between the CO groups in PBS and free OH of the A-G-G microcapsules.
32829100	2	3	theme	A-G-G	329:333	arg1	mechanism					316:324	The formation mechanism	302:324	The formation mechanism of A-G-G	302:333	The formation mechanism of A-G-G was determined by fourier-transform infrared spectroscopy, scanning electron microscopy, and other characterization methods.
32829100	3	4	theme	/A-G-G	494:499	arg1	films					511:515	polybutylene succinate (PBS)/A-G-G composite films	466:515	polybutylene succinate (PBS)/A-G-G composite films with different contents of A-G-G microcapsules	466:562	Then, polybutylene succinate (PBS)/A-G-G composite films with different contents of A-G-G microcapsules were prepared.
32829100	0	5	from	Effects	0:6	arg1	cross-linking					58:70	the mechanical, cross-linking, and release properties	42:94	cross-linking	58:70	Effects of microencapsulated abamectin on the mechanical, cross-linking, and release properties of PBS.
32829100	0	5	from	Effects	0:6	arg1	properties					85:94	the mechanical, cross-linking, and release properties	42:94	properties	85:94	Effects of microencapsulated abamectin on the mechanical, cross-linking, and release properties of PBS.
32829100	9	6	theme	good	1146:1149	arg1	performance					1164:1174	good slow-release performance	1146:1174	good slow-release performance	1146:1174	Overall, the PBS/A-G-G composite films exhibit good slow-release performance.
32829100	1	7	theme	wall	249:252	arg1	materials					254:262	the wall materials	245:262	the wall materials	245:262	Herein, nanocomposite microencapsulated abamectin (A-G-G) have been prepared by composite coacervation method with gelatin and gum arabic as the wall materials and abamectin (A-W) as core material.
32829100	4	8	from	effects	583:589	arg1	properties					661:670	the mechanical and sustained-release properties	624:670	properties	661:670	The effects of adding A-G-G microcapsules on the mechanical and sustained-release properties of the composite films were studied.
32829100	3	9	with	films	511:515	arg1	contents					532:539	different contents	522:539	different contents	522:539	Then, polybutylene succinate (PBS)/A-G-G composite films with different contents of A-G-G microcapsules were prepared.
32829100	6	10	theme	composite	909:917	arg1	films					919:923	composite films	909:923	composite films	909:923	With an increase in the A-G-G microcapsule content, the elongation at the break of composite films increases significantly.
32829100	5	11	theme	strong	738:743	arg1	interaction					745:755	a strong interaction	736:755	a strong interaction between the CO groups in PBS and free OH of the A-G-G microcapsules	736:823	Results show that there is a strong interaction between the CO groups in PBS and free OH of the A-G-G microcapsules.
32829100	2	12	theme	electron	403:410	arg1	microscopy					412:421	scanning electron microscopy	394:421	scanning electron microscopy	394:421	The formation mechanism of A-G-G was determined by fourier-transform infrared spectroscopy, scanning electron microscopy, and other characterization methods.
32829100	4	13	theme	composite	679:687	arg1	films					689:693	the composite films	675:693	the composite films	675:693	The effects of adding A-G-G microcapsules on the mechanical and sustained-release properties of the composite films were studied.
32829100	0	14	theme	PBS	99:101	arg1	cross-linking					58:70	the mechanical, cross-linking, and release properties	42:94	cross-linking	58:70	Effects of microencapsulated abamectin on the mechanical, cross-linking, and release properties of PBS.
32829100	0	14	theme	PBS	99:101	arg1	properties					85:94	the mechanical, cross-linking, and release properties	42:94	properties	85:94	Effects of microencapsulated abamectin on the mechanical, cross-linking, and release properties of PBS.
32829100	9	15	theme	slow-release	1151:1162	arg1	performance					1164:1174	good slow-release performance	1146:1174	good slow-release performance	1146:1174	Overall, the PBS/A-G-G composite films exhibit good slow-release performance.
32829100	3	16	theme	different	522:530	arg1	contents					532:539	different contents	522:539	different contents	522:539	Then, polybutylene succinate (PBS)/A-G-G composite films with different contents of A-G-G microcapsules were prepared.
32829100	8	17	theme	maximum	1057:1063	arg1	absorption					1071:1080	The maximum water absorption	1053:1080	The maximum water absorption	1053:1080	The maximum water absorption is 329 ± 5.84 %.
32829100	8	17	theme	maximum	1057:1063	arg1	%					1096:1096	329 ± 5.84 %	1085:1096	329 ± 5.84 %	1085:1096	The maximum water absorption is 329 ± 5.84 %.
32829100	2	18	theme	infrared	371:378	arg1	spectroscopy					380:391	fourier-transform infrared spectroscopy	353:391	fourier-transform infrared spectroscopy	353:391	The formation mechanism of A-G-G was determined by fourier-transform infrared spectroscopy, scanning electron microscopy, and other characterization methods.
32829100	2	19	theme	characterization	434:449	arg1	methods					451:457	other characterization methods	428:457	other characterization methods	428:457	The formation mechanism of A-G-G was determined by fourier-transform infrared spectroscopy, scanning electron microscopy, and other characterization methods.
32829100	6	20	theme	microcapsule	856:867	arg1	content					869:875	the A-G-G microcapsule content	846:875	the A-G-G microcapsule content	846:875	With an increase in the A-G-G microcapsule content, the elongation at the break of composite films increases significantly.
32829100	2	21	theme	fourier-transform	353:369	arg1	spectroscopy					380:391	fourier-transform infrared spectroscopy	353:391	fourier-transform infrared spectroscopy	353:391	The formation mechanism of A-G-G was determined by fourier-transform infrared spectroscopy, scanning electron microscopy, and other characterization methods.
32829100	2	22	theme	other	428:432	arg1	methods					451:457	other characterization methods	428:457	other characterization methods	428:457	The formation mechanism of A-G-G was determined by fourier-transform infrared spectroscopy, scanning electron microscopy, and other characterization methods.
32829100	6	23	from	increase	834:841	arg1	content					869:875	the A-G-G microcapsule content	846:875	the A-G-G microcapsule content	846:875	With an increase in the A-G-G microcapsule content, the elongation at the break of composite films increases significantly.
32829100	0	24	theme	abamectin	29:37	arg1	Effects					0:6	Effects	0:6	Effects of microencapsulated abamectin on the mechanical, cross-linking, and release properties of PBS	0:101	Effects of microencapsulated abamectin on the mechanical, cross-linking, and release properties of PBS.
32829100	1	25	with	method	207:212	arg1	gum					231:233	gum	231:233	gum	231:233	Herein, nanocomposite microencapsulated abamectin (A-G-G) have been prepared by composite coacervation method with gelatin and gum arabic as the wall materials and abamectin (A-W) as core material.
32829100	1	25	with	method	207:212	arg1	gelatin					219:225	gelatin	219:225	gelatin	219:225	Herein, nanocomposite microencapsulated abamectin (A-G-G) have been prepared by composite coacervation method with gelatin and gum arabic as the wall materials and abamectin (A-W) as core material.
32829100	1	25	with	method	207:212	arg1	A-W					279:281	A-W	279:281	A-W	279:281	Herein, nanocomposite microencapsulated abamectin (A-G-G) have been prepared by composite coacervation method with gelatin and gum arabic as the wall materials and abamectin (A-W) as core material.
32829100	1	25	with	method	207:212	arg1	abamectin					268:276	abamectin	268:276	abamectin (A-W)	268:282	Herein, nanocomposite microencapsulated abamectin (A-G-G) have been prepared by composite coacervation method with gelatin and gum arabic as the wall materials and abamectin (A-W) as core material.
32829100	3	26	theme	A-G-G	544:548	arg1	microcapsules					550:562	A-G-G microcapsules	544:562	A-G-G microcapsules	544:562	Then, polybutylene succinate (PBS)/A-G-G composite films with different contents of A-G-G microcapsules were prepared.
32829100	1	27	theme	composite	184:192	arg1	method					207:212	composite coacervation method	184:212	composite coacervation method with gelatin and gum arabic as the wall materials and abamectin (A-W)	184:282	Herein, nanocomposite microencapsulated abamectin (A-G-G) have been prepared by composite coacervation method with gelatin and gum arabic as the wall materials and abamectin (A-W) as core material.
32829100	0	28	theme	microencapsulated	11:27	arg1	abamectin					29:37	microencapsulated abamectin	11:37	microencapsulated abamectin	11:37	Effects of microencapsulated abamectin on the mechanical, cross-linking, and release properties of PBS.
32829100	6	29	theme	A-G-G	850:854	arg1	content					869:875	the A-G-G microcapsule content	846:875	the A-G-G microcapsule content	846:875	With an increase in the A-G-G microcapsule content, the elongation at the break of composite films increases significantly.
32829100	3	30	theme	microcapsules	550:562	arg1	films					511:515	polybutylene succinate (PBS)/A-G-G composite films	466:515	polybutylene succinate (PBS)/A-G-G composite films with different contents of A-G-G microcapsules	466:562	Then, polybutylene succinate (PBS)/A-G-G composite films with different contents of A-G-G microcapsules were prepared.
32829100	1	31	theme	coacervation	194:205	arg1	method					207:212	composite coacervation method	184:212	composite coacervation method with gelatin and gum arabic as the wall materials and abamectin (A-W)	184:282	Herein, nanocomposite microencapsulated abamectin (A-G-G) have been prepared by composite coacervation method with gelatin and gum arabic as the wall materials and abamectin (A-W) as core material.
32829100	7	32	theme	composite	1013:1021	arg1	films					1023:1027	the composite films	1009:1027	the composite films	1009:1027	When the A-G-G content is 15 %, the elongation at break of the composite films reaches 178.6 ± 6.26 %.
32829100	4	33	theme	sustained-release	643:659	arg1	properties					661:670	the mechanical and sustained-release properties	624:670	properties	661:670	The effects of adding A-G-G microcapsules on the mechanical and sustained-release properties of the composite films were studied.
32829100	2	34	theme	scanning	394:401	arg1	microscopy					412:421	scanning electron microscopy	394:421	scanning electron microscopy	394:421	The formation mechanism of A-G-G was determined by fourier-transform infrared spectroscopy, scanning electron microscopy, and other characterization methods.
32829100	7	35	theme	films	1023:1027	arg1	break					1000:1004	the elongation at break	982:1004	the elongation at break of the composite films	982:1027	When the A-G-G content is 15 %, the elongation at break of the composite films reaches 178.6 ± 6.26 %.
32829100	9	36	theme	PBS/A-G-G	1112:1120	arg1	films					1132:1136	the PBS/A-G-G composite films	1108:1136	the PBS/A-G-G composite films	1108:1136	Overall, the PBS/A-G-G composite films exhibit good slow-release performance.
32829100	6	37	theme	films	919:923	arg1	elongation					882:891	the elongation	878:891	the elongation at the break of composite films	878:923	With an increase in the A-G-G microcapsule content, the elongation at the break of composite films increases significantly.
32829100	5	38	theme	free	790:793	arg1	OH					795:796	free OH	790:796	free OH of the A-G-G microcapsules	790:823	Results show that there is a strong interaction between the CO groups in PBS and free OH of the A-G-G microcapsules.
32829100	7	39	theme	elongation	986:995	arg1	break					1000:1004	the elongation at break	982:1004	the elongation at break of the composite films	982:1027	When the A-G-G content is 15 %, the elongation at break of the composite films reaches 178.6 ± 6.26 %.
32829100	0	40	theme	mechanical	46:55	arg1	cross-linking					58:70	the mechanical, cross-linking, and release properties	42:94	cross-linking	58:70	Effects of microencapsulated abamectin on the mechanical, cross-linking, and release properties of PBS.
32829100	1	41	theme	core	287:290	arg1	abamectin					144:152	nanocomposite microencapsulated abamectin	112:152	nanocomposite microencapsulated abamectin (A-G-G)	112:160	Herein, nanocomposite microencapsulated abamectin (A-G-G) have been prepared by composite coacervation method with gelatin and gum arabic as the wall materials and abamectin (A-W) as core material.
32829100	1	41	theme	core	287:290	arg1	material					292:299	core material	287:299	core material	287:299	Herein, nanocomposite microencapsulated abamectin (A-G-G) have been prepared by composite coacervation method with gelatin and gum arabic as the wall materials and abamectin (A-W) as core material.
32829100	8	42	theme	water	1065:1069	arg1	absorption					1071:1080	The maximum water absorption	1053:1080	The maximum water absorption	1053:1080	The maximum water absorption is 329 ± 5.84 %.
32829100	8	42	theme	water	1065:1069	arg1	%					1096:1096	329 ± 5.84 %	1085:1096	329 ± 5.84 %	1085:1096	The maximum water absorption is 329 ± 5.84 %.
32829100	2	43	theme	formation	306:314	arg1	mechanism					316:324	The formation mechanism	302:324	The formation mechanism of A-G-G	302:333	The formation mechanism of A-G-G was determined by fourier-transform infrared spectroscopy, scanning electron microscopy, and other characterization methods.
32829100	7	44	theme	at	997:998	arg1	break					1000:1004	the elongation at break	982:1004	the elongation at break of the composite films	982:1027	When the A-G-G content is 15 %, the elongation at break of the composite films reaches 178.6 ± 6.26 %.
32829100	5	45	from	groups	772:777	arg1	PBS					782:784	PBS	782:784	PBS	782:784	Results show that there is a strong interaction between the CO groups in PBS and free OH of the A-G-G microcapsules.
32829100	5	45	from	groups	772:777	arg1	OH					795:796	free OH	790:796	free OH of the A-G-G microcapsules	790:823	Results show that there is a strong interaction between the CO groups in PBS and free OH of the A-G-G microcapsules.
32829100	1	46	theme	nanocomposite	112:124	arg1	A-G-G					155:159	A-G-G	155:159	A-G-G	155:159	Herein, nanocomposite microencapsulated abamectin (A-G-G) have been prepared by composite coacervation method with gelatin and gum arabic as the wall materials and abamectin (A-W) as core material.
32829100	1	46	theme	nanocomposite	112:124	arg1	abamectin					144:152	nanocomposite microencapsulated abamectin	112:152	nanocomposite microencapsulated abamectin (A-G-G)	112:160	Herein, nanocomposite microencapsulated abamectin (A-G-G) have been prepared by composite coacervation method with gelatin and gum arabic as the wall materials and abamectin (A-W) as core material.
32829100	1	46	theme	nanocomposite	112:124	arg1	material					292:299	core material	287:299	core material	287:299	Herein, nanocomposite microencapsulated abamectin (A-G-G) have been prepared by composite coacervation method with gelatin and gum arabic as the wall materials and abamectin (A-W) as core material.
32829100	9	47	theme	composite	1122:1130	arg1	films					1132:1136	the PBS/A-G-G composite films	1108:1136	the PBS/A-G-G composite films	1108:1136	Overall, the PBS/A-G-G composite films exhibit good slow-release performance.
32829100	4	48	theme	A-G-G	601:605	arg1	microcapsules					607:619	adding A-G-G microcapsules	594:619	adding A-G-G microcapsules	594:619	The effects of adding A-G-G microcapsules on the mechanical and sustained-release properties of the composite films were studied.
32829100	1	49	theme	microencapsulated	126:142	arg1	A-G-G					155:159	A-G-G	155:159	A-G-G	155:159	Herein, nanocomposite microencapsulated abamectin (A-G-G) have been prepared by composite coacervation method with gelatin and gum arabic as the wall materials and abamectin (A-W) as core material.
32829100	1	49	theme	microencapsulated	126:142	arg1	abamectin					144:152	nanocomposite microencapsulated abamectin	112:152	nanocomposite microencapsulated abamectin (A-G-G)	112:160	Herein, nanocomposite microencapsulated abamectin (A-G-G) have been prepared by composite coacervation method with gelatin and gum arabic as the wall materials and abamectin (A-W) as core material.
32829100	1	49	theme	microencapsulated	126:142	arg1	material					292:299	core material	287:299	core material	287:299	Herein, nanocomposite microencapsulated abamectin (A-G-G) have been prepared by composite coacervation method with gelatin and gum arabic as the wall materials and abamectin (A-W) as core material.
32829100	4	50	theme	adding	594:599	arg1	microcapsules					607:619	adding A-G-G microcapsules	594:619	adding A-G-G microcapsules	594:619	The effects of adding A-G-G microcapsules on the mechanical and sustained-release properties of the composite films were studied.
32829100	4	51	theme	mechanical	628:637	arg1	properties					661:670	the mechanical and sustained-release properties	624:670	properties	661:670	The effects of adding A-G-G microcapsules on the mechanical and sustained-release properties of the composite films were studied.
32829100	3	52	theme	composite	501:509	arg1	films					511:515	polybutylene succinate (PBS)/A-G-G composite films	466:515	polybutylene succinate (PBS)/A-G-G composite films with different contents of A-G-G microcapsules	466:562	Then, polybutylene succinate (PBS)/A-G-G composite films with different contents of A-G-G microcapsules were prepared.
32829100	7	53	from	break	1000:1004	arg1	at					997:998	the elongation at break	982:1004	the elongation at break of the composite films	982:1027	When the A-G-G content is 15 %, the elongation at break of the composite films reaches 178.6 ± 6.26 %.
32829100	7	53	from	break	1000:1004	arg1	elongation					986:995	the elongation at break	982:1004	the elongation at break of the composite films	982:1027	When the A-G-G content is 15 %, the elongation at break of the composite films reaches 178.6 ± 6.26 %.
32829100	5	54	theme	A-G-G	805:809	arg1	microcapsules					811:823	the A-G-G microcapsules	801:823	the A-G-G microcapsules	801:823	Results show that there is a strong interaction between the CO groups in PBS and free OH of the A-G-G microcapsules.
32829100	7	55	theme	A-G-G	959:963	arg1	content					965:971	the A-G-G content	955:971	the A-G-G content	955:971	When the A-G-G content is 15 %, the elongation at break of the composite films reaches 178.6 ± 6.26 %.
32829100	7	55	theme	A-G-G	959:963	arg1	%					979:979	15 %	976:979	15 %	976:979	When the A-G-G content is 15 %, the elongation at break of the composite films reaches 178.6 ± 6.26 %.
32829100	4	56	theme	films	689:693	arg1	properties					661:670	the mechanical and sustained-release properties	624:670	properties	661:670	The effects of adding A-G-G microcapsules on the mechanical and sustained-release properties of the composite films were studied.
32829100	4	57	theme	microcapsules	607:619	arg1	effects					583:589	The effects	579:589	The effects of adding A-G-G microcapsules on the mechanical and sustained-release properties of the composite films	579:693	The effects of adding A-G-G microcapsules on the mechanical and sustained-release properties of the composite films were studied.
33857510	3	0	theme	β-l,6-Galf	671:680	arg1	residues					682:689	A few β-l,6-Galf residues	665:689	A few β-l,6-Galf residues	665:689	A few β-l,6-Galf residues were also detected in the IPS fraction.
33857510	2	1	theme	~3:1	583:586	arg1	ratio					574:578	a backbone ratio	563:578	a backbone ratio of ~3:1	563:586	Their chemical structures investigated by IR, NMR, enzymolysis and methylation analysis indicate that both IPS and EPS are galactomannans composed of α-1,2- mannopyranose (Manp) and α-1,6-Manp in a backbone ratio of ~3:1, respectively, both decorated with β-l,5-galactofuranose (Galf) side chains.
33857510	1	2	theme	kDa	246:248	arg1	IPS					280:282	40.4 kDa intracellular polysaccharide, IPS	241:282	IPS	280:282	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	1	2	theme	kDa	246:248	arg1	mycelium					231:238	the mycelium	227:238	the mycelium (40.4 kDa intracellular polysaccharide, IPS)	227:283	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	0	3	from	study	12:16	arg1	galectins					139:147	galectins	139:147	galectins	139:147	Comparative study on the structures of intra- and extra-cellular polysaccharides from Penicillium oxalicum and their inhibitory effects on galectins.
33857510	0	3	from	study	12:16	arg1	structures					25:34	the structures	21:34	the structures of intra- and extra-cellular polysaccharides from Penicillium oxalicum	21:105	Comparative study on the structures of intra- and extra-cellular polysaccharides from Penicillium oxalicum and their inhibitory effects on galectins.
33857510	10	4	theme	relationships	1366:1378	arg1	understanding					1330:1342	our understanding	1326:1342	our understanding of structure-activity relationships of polysaccharides as galectin inhibitors	1326:1420	These results increase our understanding of structure-activity relationships of polysaccharides as galectin inhibitors.
33857510	5	5	theme	oxalicum	849:856	arg1	extraction					832:841	alkaline extraction	823:841	alkaline extraction of P. oxalicum	823:856	IPS obtained by alkaline extraction of P. oxalicum have been reported to be galactofuranans, a composition different from our IPS.
33857510	1	6	theme	intracellular	250:262	arg1	IPS					280:282	40.4 kDa intracellular polysaccharide, IPS	241:282	IPS	280:282	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	1	6	theme	intracellular	250:262	arg1	mycelium					231:238	the mycelium	227:238	the mycelium (40.4 kDa intracellular polysaccharide, IPS)	227:283	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	10	7	theme	structure-activity	1347:1364	arg1	relationships					1366:1378	structure-activity relationships	1347:1378	structure-activity relationships of polysaccharides as galectin inhibitors	1347:1420	These results increase our understanding of structure-activity relationships of polysaccharides as galectin inhibitors.
33857510	2	8	theme	β-l,5-galactofuranose	623:643	arg1	chains					657:662	β-l,5-galactofuranose (Galf) side chains	623:662	β-l,5-galactofuranose (Galf) side chains	623:662	Their chemical structures investigated by IR, NMR, enzymolysis and methylation analysis indicate that both IPS and EPS are galactomannans composed of α-1,2- mannopyranose (Manp) and α-1,6-Manp in a backbone ratio of ~3:1, respectively, both decorated with β-l,5-galactofuranose (Galf) side chains.
33857510	1	9	theme	polysaccharide	264:277	arg1	IPS					280:282	40.4 kDa intracellular polysaccharide, IPS	241:282	IPS	280:282	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	1	9	theme	polysaccharide	264:277	arg1	mycelium					231:238	the mycelium	227:238	the mycelium (40.4 kDa intracellular polysaccharide, IPS)	227:283	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	2	10	theme	backbone	565:572	arg1	ratio					574:578	a backbone ratio	563:578	a backbone ratio of ~3:1	563:586	Their chemical structures investigated by IR, NMR, enzymolysis and methylation analysis indicate that both IPS and EPS are galactomannans composed of α-1,2- mannopyranose (Manp) and α-1,6-Manp in a backbone ratio of ~3:1, respectively, both decorated with β-l,5-galactofuranose (Galf) side chains.
33857510	5	11	theme	different	914:922	arg1	galactofuranans					883:897	galactofuranans	883:897	galactofuranans	883:897	IPS obtained by alkaline extraction of P. oxalicum have been reported to be galactofuranans, a composition different from our IPS.
33857510	5	11	theme	different	914:922	arg1	composition					902:912	a composition	900:912	a composition different from our IPS	900:935	IPS obtained by alkaline extraction of P. oxalicum have been reported to be galactofuranans, a composition different from our IPS.
33857510	0	12	theme	inhibitory	117:126	arg1	effects					128:134	their inhibitory effects	111:134	their inhibitory effects on galectins	111:147	Comparative study on the structures of intra- and extra-cellular polysaccharides from Penicillium oxalicum and their inhibitory effects on galectins.
33857510	6	13	theme	fine	983:986	arg1	structure					988:996	the fine structure	979:996	the fine structure of EPS	979:1003	Up to now, there have been no reports on the fine structure of EPS.
33857510	1	14	theme	oxalicum	357:364	arg1	IPS					280:282	40.4 kDa intracellular polysaccharide, IPS	241:282	IPS	280:282	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	1	14	theme	oxalicum	357:364	arg1	polysaccharide					321:334	27.2 kDa extracellular polysaccharide	298:334	27.2 kDa extracellular polysaccharide	298:334	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	1	14	theme	oxalicum	357:364	arg1	culture					289:295	culture	289:295	culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum	289:364	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	1	14	theme	oxalicum	357:364	arg1	mycelium					231:238	the mycelium	227:238	the mycelium (40.4 kDa intracellular polysaccharide, IPS)	227:283	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	10	15	theme	galectin	1402:1409	arg1	inhibitors					1411:1420	galectin inhibitors	1402:1420	galectin inhibitors	1402:1420	These results increase our understanding of structure-activity relationships of polysaccharides as galectin inhibitors.
33857510	5	16	from	IPS	933:935	arg1	different					914:922	different	914:922	different	914:922	IPS obtained by alkaline extraction of P. oxalicum have been reported to be galactofuranans, a composition different from our IPS.
33857510	2	17	theme	methylation	434:444	arg1	analysis					446:453	methylation analysis	434:453	methylation analysis	434:453	Their chemical structures investigated by IR, NMR, enzymolysis and methylation analysis indicate that both IPS and EPS are galactomannans composed of α-1,2- mannopyranose (Manp) and α-1,6-Manp in a backbone ratio of ~3:1, respectively, both decorated with β-l,5-galactofuranose (Galf) side chains.
33857510	0	18	from	structures	25:34	arg1	oxalicum					98:105	Penicillium oxalicum	86:105	Penicillium oxalicum	86:105	Comparative study on the structures of intra- and extra-cellular polysaccharides from Penicillium oxalicum and their inhibitory effects on galectins.
33857510	2	19	theme	side	652:655	arg1	chains					657:662	β-l,5-galactofuranose (Galf) side chains	623:662	β-l,5-galactofuranose (Galf) side chains	623:662	Their chemical structures investigated by IR, NMR, enzymolysis and methylation analysis indicate that both IPS and EPS are galactomannans composed of α-1,2- mannopyranose (Manp) and α-1,6-Manp in a backbone ratio of ~3:1, respectively, both decorated with β-l,5-galactofuranose (Galf) side chains.
33857510	2	20	theme	chemical	373:380	arg1	structures					382:391	Their chemical structures	367:391	Their chemical structures investigated by IR, NMR, enzymolysis and methylation analysis	367:453	Their chemical structures investigated by IR, NMR, enzymolysis and methylation analysis indicate that both IPS and EPS are galactomannans composed of α-1,2- mannopyranose (Manp) and α-1,6-Manp in a backbone ratio of ~3:1, respectively, both decorated with β-l,5-galactofuranose (Galf) side chains.
33857510	0	21	theme	Comparative	0:10	arg1	study					12:16	Comparative study	0:16	Comparative study on the structures of intra- and extra-cellular polysaccharides from Penicillium oxalicum	0:105	Comparative study on the structures of intra- and extra-cellular polysaccharides from Penicillium oxalicum and their inhibitory effects on galectins.
33857510	3	22	theme	IPS	717:719	arg1	fraction					721:728	the IPS fraction	713:728	the IPS fraction	713:728	A few β-l,6-Galf residues were also detected in the IPS fraction.
33857510	1	23	attach	derived	214:220	arg1	polysaccharide					321:334	27.2 kDa extracellular polysaccharide	298:334	27.2 kDa extracellular polysaccharide	298:334	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	1	23	attach	derived	214:220	arg2	polysaccharides					198:212	polysaccharides	198:212	polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum	198:364	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	1	23	attach	derived	214:220	arg1	culture					289:295	culture	289:295	culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum	289:364	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	1	23	attach	derived	214:220	arg1	mycelium					231:238	the mycelium	227:238	the mycelium (40.4 kDa intracellular polysaccharide, IPS)	227:283	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	1	23	attach	derived	214:220	arg1	IPS					280:282	40.4 kDa intracellular polysaccharide, IPS	241:282	IPS	280:282	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	5	24	theme	alkaline	823:830	arg1	extraction					832:841	alkaline extraction	823:841	alkaline extraction of P. oxalicum	823:856	IPS obtained by alkaline extraction of P. oxalicum have been reported to be galactofuranans, a composition different from our IPS.
33857510	7	25	theme	inhibitory	1094:1103	arg1	effects					1105:1111	greater inhibitory effects	1086:1111	greater inhibitory effects	1086:1111	Our results of galectin-mediated hemagglutination demonstrate that IPS exhibits greater inhibitory effects on five galectins compared with EPS.
33857510	2	26	dep	composed	505:512	arg1	both					603:606	both	603:606	both	603:606	Their chemical structures investigated by IR, NMR, enzymolysis and methylation analysis indicate that both IPS and EPS are galactomannans composed of α-1,2- mannopyranose (Manp) and α-1,6-Manp in a backbone ratio of ~3:1, respectively, both decorated with β-l,5-galactofuranose (Galf) side chains.
33857510	2	27	theme	Galf	646:649	arg1	chains					657:662	β-l,5-galactofuranose (Galf) side chains	623:662	β-l,5-galactofuranose (Galf) side chains	623:662	Their chemical structures investigated by IR, NMR, enzymolysis and methylation analysis indicate that both IPS and EPS are galactomannans composed of α-1,2- mannopyranose (Manp) and α-1,6-Manp in a backbone ratio of ~3:1, respectively, both decorated with β-l,5-galactofuranose (Galf) side chains.
33857510	10	28	theme	polysaccharides	1383:1397	arg1	relationships					1366:1378	structure-activity relationships	1347:1378	structure-activity relationships of polysaccharides as galectin inhibitors	1347:1420	These results increase our understanding of structure-activity relationships of polysaccharides as galectin inhibitors.
33857510	4	29	theme	molecular	758:766	arg1	Mw					777:778	Mw	777:778	Mw	777:778	EPS and IPS have different molecular weights (Mw) and degrees of branching.
33857510	4	29	theme	molecular	758:766	arg1	weights					768:774	different molecular weights	748:774	different molecular weights (Mw)	748:779	EPS and IPS have different molecular weights (Mw) and degrees of branching.
33857510	1	30	dep	polysaccharide	321:334	arg1	EPS					337:339	EPS	337:339	EPS	337:339	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	2	31	dep	galactomannans	490:503	arg1	composed					505:512	composed	505:512	composed	505:512	Their chemical structures investigated by IR, NMR, enzymolysis and methylation analysis indicate that both IPS and EPS are galactomannans composed of α-1,2- mannopyranose (Manp) and α-1,6-Manp in a backbone ratio of ~3:1, respectively, both decorated with β-l,5-galactofuranose (Galf) side chains.
33857510	6	32	theme	EPS	1001:1003	arg1	structure					988:996	the fine structure	979:996	the fine structure of EPS	979:1003	Up to now, there have been no reports on the fine structure of EPS.
33857510	1	33	theme	polysaccharides	198:212	arg1	composition					183:193	composition	183:193	composition	183:193	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	1	33	theme	polysaccharides	198:212	arg1	content					171:177	content	171:177	content	171:177	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	8	34	theme	ring	1198:1201	arg1	form					1203:1206	a five-membered ring form	1182:1206	a five-membered ring form of galactose	1182:1219	In addition, we find that Galf, a five-membered ring form of galactose, can also inhibit galectins.
33857510	8	34	theme	ring	1198:1201	arg1	Galf					1176:1179	Galf	1176:1179	Galf	1176:1179	In addition, we find that Galf, a five-membered ring form of galactose, can also inhibit galectins.
33857510	3	35	located	detected	701:708	arg1	fraction					721:728	the IPS fraction	713:728	the IPS fraction	713:728	A few β-l,6-Galf residues were also detected in the IPS fraction.
33857510	3	35	located	detected	701:708	arg2	residues					682:689	A few β-l,6-Galf residues	665:689	A few β-l,6-Galf residues	665:689	A few β-l,6-Galf residues were also detected in the IPS fraction.
33857510	7	36	theme	hemagglutination	1039:1054	arg1	results					1010:1016	Our results	1006:1016	Our results of galectin-mediated hemagglutination	1006:1054	Our results of galectin-mediated hemagglutination demonstrate that IPS exhibits greater inhibitory effects on five galectins compared with EPS.
33857510	7	37	theme	greater	1086:1092	arg1	effects					1105:1111	greater inhibitory effects	1086:1111	greater inhibitory effects	1086:1111	Our results of galectin-mediated hemagglutination demonstrate that IPS exhibits greater inhibitory effects on five galectins compared with EPS.
33857510	8	38	theme	five-membered	1184:1196	arg1	form					1203:1206	a five-membered ring form	1182:1206	a five-membered ring form of galactose	1182:1219	In addition, we find that Galf, a five-membered ring form of galactose, can also inhibit galectins.
33857510	8	38	theme	five-membered	1184:1196	arg1	Galf					1176:1179	Galf	1176:1179	Galf	1176:1179	In addition, we find that Galf, a five-membered ring form of galactose, can also inhibit galectins.
33857510	9	39	theme	new	1268:1270	arg1	source					1272:1277	a new source	1266:1277	a new source of galectin inhibitors	1266:1300	IPS may provide a new source of galectin inhibitors.
33857510	6	40	from	reports	968:974	arg1	structure					988:996	the fine structure	979:996	the fine structure of EPS	979:1003	Up to now, there have been no reports on the fine structure of EPS.
33857510	4	41	theme	branching	796:804	arg1	Mw					777:778	Mw	777:778	Mw	777:778	EPS and IPS have different molecular weights (Mw) and degrees of branching.
33857510	4	41	theme	branching	796:804	arg1	degrees					785:791	degrees	785:791	degrees of branching	785:804	EPS and IPS have different molecular weights (Mw) and degrees of branching.
33857510	4	41	theme	branching	796:804	arg1	weights					768:774	different molecular weights	748:774	different molecular weights (Mw)	748:779	EPS and IPS have different molecular weights (Mw) and degrees of branching.
33857510	0	42	theme	intra-	39:44	arg1	polysaccharides					65:79	intra- and extra-cellular polysaccharides	39:79	intra- and extra-cellular polysaccharides from Penicillium oxalicum	39:105	Comparative study on the structures of intra- and extra-cellular polysaccharides from Penicillium oxalicum and their inhibitory effects on galectins.
33857510	1	43	theme	kDa	303:305	arg1	polysaccharide					321:334	27.2 kDa extracellular polysaccharide	298:334	27.2 kDa extracellular polysaccharide	298:334	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	1	43	theme	kDa	303:305	arg1	culture					289:295	culture	289:295	culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum	289:364	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	1	44	dep	content	171:177	arg1	the					167:169	the	167:169	the	167:169	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	1	45	theme	extracellular	307:319	arg1	polysaccharide					321:334	27.2 kDa extracellular polysaccharide	298:334	27.2 kDa extracellular polysaccharide	298:334	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	1	45	theme	extracellular	307:319	arg1	culture					289:295	culture	289:295	culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum	289:364	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
33857510	4	46	theme	different	748:756	arg1	Mw					777:778	Mw	777:778	Mw	777:778	EPS and IPS have different molecular weights (Mw) and degrees of branching.
33857510	4	46	theme	different	748:756	arg1	weights					768:774	different molecular weights	748:774	different molecular weights (Mw)	748:779	EPS and IPS have different molecular weights (Mw) and degrees of branching.
33857510	9	47	theme	galectin	1282:1289	arg1	inhibitors					1291:1300	galectin inhibitors	1282:1300	galectin inhibitors	1282:1300	IPS may provide a new source of galectin inhibitors.
33857510	8	48	theme	galactose	1211:1219	arg1	form					1203:1206	a five-membered ring form	1182:1206	a five-membered ring form of galactose	1182:1219	In addition, we find that Galf, a five-membered ring form of galactose, can also inhibit galectins.
33857510	8	48	theme	galactose	1211:1219	arg1	Galf					1176:1179	Galf	1176:1179	Galf	1176:1179	In addition, we find that Galf, a five-membered ring form of galactose, can also inhibit galectins.
33857510	9	49	theme	inhibitors	1291:1300	arg1	source					1272:1277	a new source	1266:1277	a new source of galectin inhibitors	1266:1300	IPS may provide a new source of galectin inhibitors.
33857510	0	50	theme	polysaccharides	65:79	arg1	structures					25:34	the structures	21:34	the structures of intra- and extra-cellular polysaccharides from Penicillium oxalicum	21:105	Comparative study on the structures of intra- and extra-cellular polysaccharides from Penicillium oxalicum and their inhibitory effects on galectins.
33857510	0	51	from	oxalicum	98:105	arg1	polysaccharides					65:79	intra- and extra-cellular polysaccharides	39:79	intra- and extra-cellular polysaccharides from Penicillium oxalicum	39:105	Comparative study on the structures of intra- and extra-cellular polysaccharides from Penicillium oxalicum and their inhibitory effects on galectins.
33857510	0	51	from	oxalicum	98:105	arg1	structures					25:34	the structures	21:34	the structures of intra- and extra-cellular polysaccharides from Penicillium oxalicum	21:105	Comparative study on the structures of intra- and extra-cellular polysaccharides from Penicillium oxalicum and their inhibitory effects on galectins.
33857510	4	52	contain	have	743:746	arg1	EPS					731:733	EPS	731:733	EPS	731:733	EPS and IPS have different molecular weights (Mw) and degrees of branching.
33857510	4	52	contain	have	743:746	arg2	Mw					777:778	Mw	777:778	Mw	777:778	EPS and IPS have different molecular weights (Mw) and degrees of branching.
33857510	4	52	contain	have	743:746	arg1	IPS					739:741	IPS	739:741	IPS	739:741	EPS and IPS have different molecular weights (Mw) and degrees of branching.
33857510	4	52	contain	have	743:746	arg2	weights					768:774	different molecular weights	748:774	different molecular weights (Mw)	748:779	EPS and IPS have different molecular weights (Mw) and degrees of branching.
33857510	4	52	contain	have	743:746	arg2	degrees					785:791	degrees	785:791	degrees of branching	785:804	EPS and IPS have different molecular weights (Mw) and degrees of branching.
33857510	0	53	theme	extra-cellular	50:63	arg1	polysaccharides					65:79	intra- and extra-cellular polysaccharides	39:79	intra- and extra-cellular polysaccharides from Penicillium oxalicum	39:105	Comparative study on the structures of intra- and extra-cellular polysaccharides from Penicillium oxalicum and their inhibitory effects on galectins.
33857510	3	54	theme	few	667:669	arg1	residues					682:689	A few β-l,6-Galf residues	665:689	A few β-l,6-Galf residues	665:689	A few β-l,6-Galf residues were also detected in the IPS fraction.
33857510	0	55	from	effects	128:134	arg1	galectins					139:147	galectins	139:147	galectins	139:147	Comparative study on the structures of intra- and extra-cellular polysaccharides from Penicillium oxalicum and their inhibitory effects on galectins.
33857510	0	55	from	effects	128:134	arg1	structures					25:34	the structures	21:34	the structures of intra- and extra-cellular polysaccharides from Penicillium oxalicum	21:105	Comparative study on the structures of intra- and extra-cellular polysaccharides from Penicillium oxalicum and their inhibitory effects on galectins.
33857510	7	56	theme	galectin-mediated	1021:1037	arg1	hemagglutination					1039:1054	galectin-mediated hemagglutination	1021:1054	galectin-mediated hemagglutination	1021:1054	Our results of galectin-mediated hemagglutination demonstrate that IPS exhibits greater inhibitory effects on five galectins compared with EPS.
33857510	1	57	theme	40.4	241:244	arg1	kDa					246:248	kDa	246:248	kDa	246:248	Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum.
32853607	5	0	theme	composites	818:827	arg1	kinetics					806:813	the curing kinetics	795:813	the curing kinetics of composites based on BA-a	795:841	The investigation of the effect of the treated fibers on the curing kinetics of composites based on BA-a was performed by differential scanning calorimetry (DSC) technique, under non-isothermal conditions, and isoconversional integral kinetic methods.
32853607	7	1	theme	curing	1159:1164	arg1	reactions					1166:1174	the curing reactions	1155:1174	the curing reactions	1155:1174	The Avrami-Erofeev autocatalytic kinetic model was the most appropriate to describe the curing reactions and the predicted curves from the calculated kinetic parameters fitted well with experimental data.
32853607	0	2	from	Characterization	0:15	arg1	kinetics					101:108	the curing kinetics	90:108	the curing kinetics of bisphenol A-based benzoxazine	90:141	Characterization of raw and treated Arundo donax L. cellulosic fibers and their effect on the curing kinetics of bisphenol A-based benzoxazine.
32853607	3	3	theme	structural	459:468	arg1	properties					512:521	the structural, thermal, crystallinity and morphological properties	455:521	the structural, thermal, crystallinity and morphological properties of the untreated (NRF) and treated fibers (TRF)	455:569	The examination of the structural, thermal, crystallinity and morphological properties of the untreated (NRF) and treated fibers (TRF) was carried out using different analytical techniques.
32853607	3	4	theme	crystallinity	480:492	arg1	properties					512:521	the structural, thermal, crystallinity and morphological properties	455:521	the structural, thermal, crystallinity and morphological properties of the untreated (NRF) and treated fibers (TRF)	455:569	The examination of the structural, thermal, crystallinity and morphological properties of the untreated (NRF) and treated fibers (TRF) was carried out using different analytical techniques.
32853607	5	5	theme	calorimetry	882:892	arg1	technique					900:908	differential scanning calorimetry (DSC) technique	860:908	differential scanning calorimetry (DSC) technique	860:908	The investigation of the effect of the treated fibers on the curing kinetics of composites based on BA-a was performed by differential scanning calorimetry (DSC) technique, under non-isothermal conditions, and isoconversional integral kinetic methods.
32853607	0	6	theme	effect	80:85	arg1	Characterization					0:15	Characterization	0:15	Characterization of raw and treated Arundo donax L. cellulosic fibers and their effect on the curing kinetics of bisphenol A-based benzoxazine.	0:142	Characterization of raw and treated Arundo donax L. cellulosic fibers and their effect on the curing kinetics of bisphenol A-based benzoxazine.
32853607	5	7	from	kinetics	806:813	arg1	investigation					742:754	The investigation	738:754	The investigation of the effect of the treated fibers on the curing kinetics of composites based on BA-a	738:841	The investigation of the effect of the treated fibers on the curing kinetics of composites based on BA-a was performed by differential scanning calorimetry (DSC) technique, under non-isothermal conditions, and isoconversional integral kinetic methods.
32853607	6	8	theme	curing	1016:1021	arg1	heat					1008:1011	the heat	1004:1011	the heat of curing	1004:1021	A decrease in the heat of curing as well as activation energy (Ea) was reported.
32853607	5	9	theme	fibers	785:790	arg1	effect					763:768	the effect	759:768	the effect of the treated fibers on the curing kinetics of composites based on BA-a	759:841	The investigation of the effect of the treated fibers on the curing kinetics of composites based on BA-a was performed by differential scanning calorimetry (DSC) technique, under non-isothermal conditions, and isoconversional integral kinetic methods.
32853607	0	10	theme	curing	94:99	arg1	kinetics					101:108	the curing kinetics	90:108	the curing kinetics of bisphenol A-based benzoxazine	90:141	Characterization of raw and treated Arundo donax L. cellulosic fibers and their effect on the curing kinetics of bisphenol A-based benzoxazine.
32853607	2	11	theme	chemical	355:362	arg1	treatments					364:373	different chemical treatments	345:373	different chemical treatments using either alkaline, silane or their combining treatments	345:433	RF were extracted from giant reed cane, and subjected to different chemical treatments using either alkaline, silane or their combining treatments.
32853607	3	12	theme	thermal	471:477	arg1	properties					512:521	the structural, thermal, crystallinity and morphological properties	455:521	the structural, thermal, crystallinity and morphological properties of the untreated (NRF) and treated fibers (TRF)	455:569	The examination of the structural, thermal, crystallinity and morphological properties of the untreated (NRF) and treated fibers (TRF) was carried out using different analytical techniques.
32853607	1	13	theme	present	151:157	arg1	work					159:162	the present work	147:162	the present work	147:162	In the present work, giant reed cellulosic fibers (Arundo donax L., RF) were explored as reinforcement of bisphenol A-based benzoxazine (BA-a).
32853607	2	14	theme	combining	414:422	arg1	treatments					424:433	their combining treatments	408:433	their combining treatments	408:433	RF were extracted from giant reed cane, and subjected to different chemical treatments using either alkaline, silane or their combining treatments.
32853607	7	15	from	parameters	1229:1238	arg1	reactions					1166:1174	the curing reactions	1155:1174	the curing reactions	1155:1174	The Avrami-Erofeev autocatalytic kinetic model was the most appropriate to describe the curing reactions and the predicted curves from the calculated kinetic parameters fitted well with experimental data.
32853607	7	15	from	parameters	1229:1238	arg1	curves					1194:1199	the predicted curves	1180:1199	the predicted curves from the calculated kinetic parameters fitted well with experimental data	1180:1273	The Avrami-Erofeev autocatalytic kinetic model was the most appropriate to describe the curing reactions and the predicted curves from the calculated kinetic parameters fitted well with experimental data.
32853607	7	16	theme	kinetic	1104:1110	arg1	appropriate					1131:1141	appropriate	1131:1141	appropriate	1131:1141	The Avrami-Erofeev autocatalytic kinetic model was the most appropriate to describe the curing reactions and the predicted curves from the calculated kinetic parameters fitted well with experimental data.
32853607	7	16	theme	kinetic	1104:1110	arg1	model					1112:1116	The Avrami-Erofeev autocatalytic kinetic model	1071:1116	The Avrami-Erofeev autocatalytic kinetic model	1071:1116	The Avrami-Erofeev autocatalytic kinetic model was the most appropriate to describe the curing reactions and the predicted curves from the calculated kinetic parameters fitted well with experimental data.
32853607	7	17	theme	Avrami-Erofeev	1075:1088	arg1	appropriate					1131:1141	appropriate	1131:1141	appropriate	1131:1141	The Avrami-Erofeev autocatalytic kinetic model was the most appropriate to describe the curing reactions and the predicted curves from the calculated kinetic parameters fitted well with experimental data.
32853607	7	17	theme	Avrami-Erofeev	1075:1088	arg1	model					1112:1116	The Avrami-Erofeev autocatalytic kinetic model	1071:1116	The Avrami-Erofeev autocatalytic kinetic model	1071:1116	The Avrami-Erofeev autocatalytic kinetic model was the most appropriate to describe the curing reactions and the predicted curves from the calculated kinetic parameters fitted well with experimental data.
32853607	5	18	from	investigation	742:754	arg1	kinetics					806:813	the curing kinetics	795:813	the curing kinetics of composites based on BA-a	795:841	The investigation of the effect of the treated fibers on the curing kinetics of composites based on BA-a was performed by differential scanning calorimetry (DSC) technique, under non-isothermal conditions, and isoconversional integral kinetic methods.
32853607	4	19	theme	fibers	665:670	arg1	morphology					639:648	the morphology	635:648	the morphology of the treated fibers	635:670	Broadly, the morphology of the treated fibers is affected, their crystallinity and thermal stability increased.
32853607	7	20	theme	autocatalytic	1090:1102	arg1	appropriate					1131:1141	appropriate	1131:1141	appropriate	1131:1141	The Avrami-Erofeev autocatalytic kinetic model was the most appropriate to describe the curing reactions and the predicted curves from the calculated kinetic parameters fitted well with experimental data.
32853607	7	20	theme	autocatalytic	1090:1102	arg1	model					1112:1116	The Avrami-Erofeev autocatalytic kinetic model	1071:1116	The Avrami-Erofeev autocatalytic kinetic model	1071:1116	The Avrami-Erofeev autocatalytic kinetic model was the most appropriate to describe the curing reactions and the predicted curves from the calculated kinetic parameters fitted well with experimental data.
32853607	4	21	theme	treated	657:663	arg1	fibers					665:670	the treated fibers	653:670	the treated fibers	653:670	Broadly, the morphology of the treated fibers is affected, their crystallinity and thermal stability increased.
32853607	7	22	theme	calculated	1210:1219	arg1	parameters					1229:1238	the calculated kinetic parameters	1206:1238	the calculated kinetic parameters fitted well with experimental data	1206:1273	The Avrami-Erofeev autocatalytic kinetic model was the most appropriate to describe the curing reactions and the predicted curves from the calculated kinetic parameters fitted well with experimental data.
32853607	1	23	theme	giant	165:169	arg1	L.					208:209	L.	208:209	L.	208:209	In the present work, giant reed cellulosic fibers (Arundo donax L., RF) were explored as reinforcement of bisphenol A-based benzoxazine (BA-a).
32853607	1	23	theme	giant	165:169	arg1	fibers					187:192	giant reed cellulosic fibers	165:192	giant reed cellulosic fibers (Arundo donax L., RF)	165:214	In the present work, giant reed cellulosic fibers (Arundo donax L., RF) were explored as reinforcement of bisphenol A-based benzoxazine (BA-a).
32853607	1	23	theme	giant	165:169	arg1	reinforcement					233:245	reinforcement	233:245	reinforcement of bisphenol A-based benzoxazine (BA-a)	233:285	In the present work, giant reed cellulosic fibers (Arundo donax L., RF) were explored as reinforcement of bisphenol A-based benzoxazine (BA-a).
32853607	3	24	dep	untreated	530:538	arg1	NRF					541:543	NRF	541:543	NRF	541:543	The examination of the structural, thermal, crystallinity and morphological properties of the untreated (NRF) and treated fibers (TRF) was carried out using different analytical techniques.
32853607	3	25	theme	morphological	498:510	arg1	properties					512:521	the structural, thermal, crystallinity and morphological properties	455:521	the structural, thermal, crystallinity and morphological properties of the untreated (NRF) and treated fibers (TRF)	455:569	The examination of the structural, thermal, crystallinity and morphological properties of the untreated (NRF) and treated fibers (TRF) was carried out using different analytical techniques.
32853607	1	26	theme	reed	171:174	arg1	L.					208:209	L.	208:209	L.	208:209	In the present work, giant reed cellulosic fibers (Arundo donax L., RF) were explored as reinforcement of bisphenol A-based benzoxazine (BA-a).
32853607	1	26	theme	reed	171:174	arg1	fibers					187:192	giant reed cellulosic fibers	165:192	giant reed cellulosic fibers (Arundo donax L., RF)	165:214	In the present work, giant reed cellulosic fibers (Arundo donax L., RF) were explored as reinforcement of bisphenol A-based benzoxazine (BA-a).
32853607	1	26	theme	reed	171:174	arg1	reinforcement					233:245	reinforcement	233:245	reinforcement of bisphenol A-based benzoxazine (BA-a)	233:285	In the present work, giant reed cellulosic fibers (Arundo donax L., RF) were explored as reinforcement of bisphenol A-based benzoxazine (BA-a).
32853607	0	27	theme	raw	20:22	arg1	fibers					63:68	raw and treated Arundo donax L. cellulosic fibers	20:68	fibers	63:68	Characterization of raw and treated Arundo donax L. cellulosic fibers and their effect on the curing kinetics of bisphenol A-based benzoxazine.
32853607	5	28	theme	differential	860:871	arg1	DSC					895:897	DSC	895:897	DSC	895:897	The investigation of the effect of the treated fibers on the curing kinetics of composites based on BA-a was performed by differential scanning calorimetry (DSC) technique, under non-isothermal conditions, and isoconversional integral kinetic methods.
32853607	5	28	theme	differential	860:871	arg1	calorimetry					882:892	differential scanning calorimetry	860:892	differential scanning calorimetry (DSC) technique	860:908	The investigation of the effect of the treated fibers on the curing kinetics of composites based on BA-a was performed by differential scanning calorimetry (DSC) technique, under non-isothermal conditions, and isoconversional integral kinetic methods.
32853607	1	29	dep	L.	208:209	arg1	RF					212:213	RF	212:213	RF	212:213	In the present work, giant reed cellulosic fibers (Arundo donax L., RF) were explored as reinforcement of bisphenol A-based benzoxazine (BA-a).
32853607	3	30	theme	properties	512:521	arg1	examination					440:450	The examination	436:450	The examination of the structural, thermal, crystallinity and morphological properties of the untreated (NRF) and treated fibers (TRF)	436:569	The examination of the structural, thermal, crystallinity and morphological properties of the untreated (NRF) and treated fibers (TRF) was carried out using different analytical techniques.
32853607	5	31	theme	isoconversional	948:962	arg1	methods					981:987	isoconversional integral kinetic methods	948:987	isoconversional integral kinetic methods	948:987	The investigation of the effect of the treated fibers on the curing kinetics of composites based on BA-a was performed by differential scanning calorimetry (DSC) technique, under non-isothermal conditions, and isoconversional integral kinetic methods.
32853607	1	32	theme	cellulosic	176:185	arg1	L.					208:209	L.	208:209	L.	208:209	In the present work, giant reed cellulosic fibers (Arundo donax L., RF) were explored as reinforcement of bisphenol A-based benzoxazine (BA-a).
32853607	1	32	theme	cellulosic	176:185	arg1	fibers					187:192	giant reed cellulosic fibers	165:192	giant reed cellulosic fibers (Arundo donax L., RF)	165:214	In the present work, giant reed cellulosic fibers (Arundo donax L., RF) were explored as reinforcement of bisphenol A-based benzoxazine (BA-a).
32853607	1	32	theme	cellulosic	176:185	arg1	reinforcement					233:245	reinforcement	233:245	reinforcement of bisphenol A-based benzoxazine (BA-a)	233:285	In the present work, giant reed cellulosic fibers (Arundo donax L., RF) were explored as reinforcement of bisphenol A-based benzoxazine (BA-a).
32853607	0	33	theme	Arundo	36:41	arg1	fibers					63:68	raw and treated Arundo donax L. cellulosic fibers	20:68	fibers	63:68	Characterization of raw and treated Arundo donax L. cellulosic fibers and their effect on the curing kinetics of bisphenol A-based benzoxazine.
32853607	1	34	theme	bisphenol	250:258	arg1	benzoxazine					268:278	bisphenol A-based benzoxazine	250:278	bisphenol A-based benzoxazine (BA-a)	250:285	In the present work, giant reed cellulosic fibers (Arundo donax L., RF) were explored as reinforcement of bisphenol A-based benzoxazine (BA-a).
32853607	1	34	theme	bisphenol	250:258	arg1	BA-a					281:284	BA-a	281:284	BA-a	281:284	In the present work, giant reed cellulosic fibers (Arundo donax L., RF) were explored as reinforcement of bisphenol A-based benzoxazine (BA-a).
32853607	0	35	theme	A-based	123:129	arg1	benzoxazine					131:141	bisphenol A-based benzoxazine	113:141	bisphenol A-based benzoxazine	113:141	Characterization of raw and treated Arundo donax L. cellulosic fibers and their effect on the curing kinetics of bisphenol A-based benzoxazine.
32853607	5	36	theme	kinetic	973:979	arg1	methods					981:987	isoconversional integral kinetic methods	948:987	isoconversional integral kinetic methods	948:987	The investigation of the effect of the treated fibers on the curing kinetics of composites based on BA-a was performed by differential scanning calorimetry (DSC) technique, under non-isothermal conditions, and isoconversional integral kinetic methods.
32853607	5	37	theme	curing	799:804	arg1	kinetics					806:813	the curing kinetics	795:813	the curing kinetics of composites based on BA-a	795:841	The investigation of the effect of the treated fibers on the curing kinetics of composites based on BA-a was performed by differential scanning calorimetry (DSC) technique, under non-isothermal conditions, and isoconversional integral kinetic methods.
32853607	2	38	theme	different	345:353	arg1	treatments					364:373	different chemical treatments	345:373	different chemical treatments using either alkaline, silane or their combining treatments	345:433	RF were extracted from giant reed cane, and subjected to different chemical treatments using either alkaline, silane or their combining treatments.
32853607	0	39	theme	treated	28:34	arg1	fibers					63:68	raw and treated Arundo donax L. cellulosic fibers	20:68	fibers	63:68	Characterization of raw and treated Arundo donax L. cellulosic fibers and their effect on the curing kinetics of bisphenol A-based benzoxazine.
32853607	1	40	theme	A-based	260:266	arg1	benzoxazine					268:278	bisphenol A-based benzoxazine	250:278	bisphenol A-based benzoxazine (BA-a)	250:285	In the present work, giant reed cellulosic fibers (Arundo donax L., RF) were explored as reinforcement of bisphenol A-based benzoxazine (BA-a).
32853607	1	40	theme	A-based	260:266	arg1	BA-a					281:284	BA-a	281:284	BA-a	281:284	In the present work, giant reed cellulosic fibers (Arundo donax L., RF) were explored as reinforcement of bisphenol A-based benzoxazine (BA-a).
32853607	0	41	theme	bisphenol	113:121	arg1	benzoxazine					131:141	bisphenol A-based benzoxazine	113:141	bisphenol A-based benzoxazine	113:141	Characterization of raw and treated Arundo donax L. cellulosic fibers and their effect on the curing kinetics of bisphenol A-based benzoxazine.
32853607	0	42	from	fibers	63:68	arg1	kinetics					101:108	the curing kinetics	90:108	the curing kinetics of bisphenol A-based benzoxazine	90:141	Characterization of raw and treated Arundo donax L. cellulosic fibers and their effect on the curing kinetics of bisphenol A-based benzoxazine.
32853607	5	43	theme	treated	777:783	arg1	fibers					785:790	the treated fibers	773:790	the treated fibers	773:790	The investigation of the effect of the treated fibers on the curing kinetics of composites based on BA-a was performed by differential scanning calorimetry (DSC) technique, under non-isothermal conditions, and isoconversional integral kinetic methods.
32853607	0	44	theme	L.	49:50	arg1	fibers					63:68	raw and treated Arundo donax L. cellulosic fibers	20:68	fibers	63:68	Characterization of raw and treated Arundo donax L. cellulosic fibers and their effect on the curing kinetics of bisphenol A-based benzoxazine.
32853607	1	45	theme	benzoxazine	268:278	arg1	fibers					187:192	giant reed cellulosic fibers	165:192	giant reed cellulosic fibers (Arundo donax L., RF)	165:214	In the present work, giant reed cellulosic fibers (Arundo donax L., RF) were explored as reinforcement of bisphenol A-based benzoxazine (BA-a).
32853607	1	45	theme	benzoxazine	268:278	arg1	reinforcement					233:245	reinforcement	233:245	reinforcement of bisphenol A-based benzoxazine (BA-a)	233:285	In the present work, giant reed cellulosic fibers (Arundo donax L., RF) were explored as reinforcement of bisphenol A-based benzoxazine (BA-a).
32853607	3	46	theme	untreated	530:538	arg1	TRF					566:568	TRF	566:568	TRF	566:568	The examination of the structural, thermal, crystallinity and morphological properties of the untreated (NRF) and treated fibers (TRF) was carried out using different analytical techniques.
32853607	3	46	theme	untreated	530:538	arg1	fibers					558:563	the untreated (NRF) and treated fibers	526:563	the untreated (NRF) and treated fibers (TRF)	526:569	The examination of the structural, thermal, crystallinity and morphological properties of the untreated (NRF) and treated fibers (TRF) was carried out using different analytical techniques.
32853607	0	47	theme	donax	43:47	arg1	fibers					63:68	raw and treated Arundo donax L. cellulosic fibers	20:68	fibers	63:68	Characterization of raw and treated Arundo donax L. cellulosic fibers and their effect on the curing kinetics of bisphenol A-based benzoxazine.
32853607	0	48	theme	benzoxazine	131:141	arg1	kinetics					101:108	the curing kinetics	90:108	the curing kinetics of bisphenol A-based benzoxazine	90:141	Characterization of raw and treated Arundo donax L. cellulosic fibers and their effect on the curing kinetics of bisphenol A-based benzoxazine.
32853607	3	49	theme	different	593:601	arg1	techniques					614:623	different analytical techniques	593:623	different analytical techniques	593:623	The examination of the structural, thermal, crystallinity and morphological properties of the untreated (NRF) and treated fibers (TRF) was carried out using different analytical techniques.
32853607	5	50	theme	integral	964:971	arg1	methods					981:987	isoconversional integral kinetic methods	948:987	isoconversional integral kinetic methods	948:987	The investigation of the effect of the treated fibers on the curing kinetics of composites based on BA-a was performed by differential scanning calorimetry (DSC) technique, under non-isothermal conditions, and isoconversional integral kinetic methods.
32853607	5	51	from	effect	763:768	arg1	kinetics					806:813	the curing kinetics	795:813	the curing kinetics of composites based on BA-a	795:841	The investigation of the effect of the treated fibers on the curing kinetics of composites based on BA-a was performed by differential scanning calorimetry (DSC) technique, under non-isothermal conditions, and isoconversional integral kinetic methods.
32853607	3	52	theme	analytical	603:612	arg1	techniques					614:623	different analytical techniques	593:623	different analytical techniques	593:623	The examination of the structural, thermal, crystallinity and morphological properties of the untreated (NRF) and treated fibers (TRF) was carried out using different analytical techniques.
32853607	3	53	theme	fibers	558:563	arg1	properties					512:521	the structural, thermal, crystallinity and morphological properties	455:521	the structural, thermal, crystallinity and morphological properties of the untreated (NRF) and treated fibers (TRF)	455:569	The examination of the structural, thermal, crystallinity and morphological properties of the untreated (NRF) and treated fibers (TRF) was carried out using different analytical techniques.
32853607	6	54	from	decrease	992:999	arg1	heat					1008:1011	the heat	1004:1011	the heat of curing	1004:1021	A decrease in the heat of curing as well as activation energy (Ea) was reported.
32853607	6	55	theme	activation	1034:1043	arg1	Ea					1053:1054	Ea	1053:1054	Ea	1053:1054	A decrease in the heat of curing as well as activation energy (Ea) was reported.
32853607	6	55	theme	activation	1034:1043	arg1	energy					1045:1050	activation energy	1034:1050	A decrease in the heat of curing as well as activation energy (Ea)	990:1055	A decrease in the heat of curing as well as activation energy (Ea) was reported.
32853607	5	56	theme	scanning	873:880	arg1	DSC					895:897	DSC	895:897	DSC	895:897	The investigation of the effect of the treated fibers on the curing kinetics of composites based on BA-a was performed by differential scanning calorimetry (DSC) technique, under non-isothermal conditions, and isoconversional integral kinetic methods.
32853607	5	56	theme	scanning	873:880	arg1	calorimetry					882:892	differential scanning calorimetry	860:892	differential scanning calorimetry (DSC) technique	860:908	The investigation of the effect of the treated fibers on the curing kinetics of composites based on BA-a was performed by differential scanning calorimetry (DSC) technique, under non-isothermal conditions, and isoconversional integral kinetic methods.
32853607	6	57	from	energy	1045:1050	arg1	heat					1008:1011	the heat	1004:1011	the heat of curing	1004:1021	A decrease in the heat of curing as well as activation energy (Ea) was reported.
32853607	4	58	theme	thermal	709:715	arg1	stability					717:725	thermal stability	709:725	thermal stability	709:725	Broadly, the morphology of the treated fibers is affected, their crystallinity and thermal stability increased.
32853607	7	59	theme	predicted	1184:1192	arg1	curves					1194:1199	the predicted curves	1180:1199	the predicted curves from the calculated kinetic parameters fitted well with experimental data	1180:1273	The Avrami-Erofeev autocatalytic kinetic model was the most appropriate to describe the curing reactions and the predicted curves from the calculated kinetic parameters fitted well with experimental data.
32853607	7	60	theme	experimental	1257:1268	arg1	data					1270:1273	experimental data	1257:1273	experimental data	1257:1273	The Avrami-Erofeev autocatalytic kinetic model was the most appropriate to describe the curing reactions and the predicted curves from the calculated kinetic parameters fitted well with experimental data.
32853607	0	61	theme	fibers	63:68	arg1	Characterization					0:15	Characterization	0:15	Characterization of raw and treated Arundo donax L. cellulosic fibers and their effect on the curing kinetics of bisphenol A-based benzoxazine.	0:142	Characterization of raw and treated Arundo donax L. cellulosic fibers and their effect on the curing kinetics of bisphenol A-based benzoxazine.
32853607	0	62	from	effect	80:85	arg1	kinetics					101:108	the curing kinetics	90:108	the curing kinetics of bisphenol A-based benzoxazine	90:141	Characterization of raw and treated Arundo donax L. cellulosic fibers and their effect on the curing kinetics of bisphenol A-based benzoxazine.
32853607	5	63	theme	non-isothermal	917:930	arg1	conditions					932:941	non-isothermal conditions	917:941	non-isothermal conditions	917:941	The investigation of the effect of the treated fibers on the curing kinetics of composites based on BA-a was performed by differential scanning calorimetry (DSC) technique, under non-isothermal conditions, and isoconversional integral kinetic methods.
32853607	5	64	theme	effect	763:768	arg1	investigation					742:754	The investigation	738:754	The investigation of the effect of the treated fibers on the curing kinetics of composites based on BA-a	738:841	The investigation of the effect of the treated fibers on the curing kinetics of composites based on BA-a was performed by differential scanning calorimetry (DSC) technique, under non-isothermal conditions, and isoconversional integral kinetic methods.
32853607	2	65	theme	reed	317:320	arg1	cane					322:325	giant reed cane	311:325	giant reed cane	311:325	RF were extracted from giant reed cane, and subjected to different chemical treatments using either alkaline, silane or their combining treatments.
32853607	7	66	theme	kinetic	1221:1227	arg1	parameters					1229:1238	the calculated kinetic parameters	1206:1238	the calculated kinetic parameters fitted well with experimental data	1206:1273	The Avrami-Erofeev autocatalytic kinetic model was the most appropriate to describe the curing reactions and the predicted curves from the calculated kinetic parameters fitted well with experimental data.
32853607	0	67	theme	cellulosic	52:61	arg1	fibers					63:68	raw and treated Arundo donax L. cellulosic fibers	20:68	fibers	63:68	Characterization of raw and treated Arundo donax L. cellulosic fibers and their effect on the curing kinetics of bisphenol A-based benzoxazine.
32853607	3	68	theme	treated	550:556	arg1	TRF					566:568	TRF	566:568	TRF	566:568	The examination of the structural, thermal, crystallinity and morphological properties of the untreated (NRF) and treated fibers (TRF) was carried out using different analytical techniques.
32853607	3	68	theme	treated	550:556	arg1	fibers					558:563	the untreated (NRF) and treated fibers	526:563	the untreated (NRF) and treated fibers (TRF)	526:569	The examination of the structural, thermal, crystallinity and morphological properties of the untreated (NRF) and treated fibers (TRF) was carried out using different analytical techniques.
32853607	2	69	theme	giant	311:315	arg1	cane					322:325	giant reed cane	311:325	giant reed cane	311:325	RF were extracted from giant reed cane, and subjected to different chemical treatments using either alkaline, silane or their combining treatments.
32853607	0	70	from	kinetics	101:108	arg1	Characterization					0:15	Characterization	0:15	Characterization of raw and treated Arundo donax L. cellulosic fibers and their effect on the curing kinetics of bisphenol A-based benzoxazine.	0:142	Characterization of raw and treated Arundo donax L. cellulosic fibers and their effect on the curing kinetics of bisphenol A-based benzoxazine.
34517029	6	0	theme	adsorption	1036:1045	arg1	process					1047:1053	the adsorption process	1032:1053	the adsorption process	1032:1053	It was found that the adsorption process follows a Freundlich adsorption isotherm and pseudo second order kinetics.
34517029	4	1	link	cross-linked	597:608	arg1	adsorbent					727:735	adsorbent	727:735	adsorbent	727:735	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	4	1	link	cross-linked	597:608	arg1	chitosan-alginate					610:626	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite	555:636	chitosan-alginate	610:626	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	7	2	from	enthalpy-driven	1196:1210	arg1	nature					1231:1236	nature	1231:1236	nature	1231:1236	A thermodynamic study revealed that the process of adsorption was enthalpy-driven and spontaneous in nature.
34517029	1	3	theme	well-known	177:186	arg1	alginate					221:228	alginate	221:228	alginate	221:228	We present an approach for synthesis of a micro-porous composite of two well-known biopolymers, namely chitosan and alginate, using glutaraldehyde as the cross-linking agent.
34517029	1	3	theme	well-known	177:186	arg1	biopolymers					188:198	two well-known biopolymers	173:198	two well-known biopolymers	173:198	We present an approach for synthesis of a micro-porous composite of two well-known biopolymers, namely chitosan and alginate, using glutaraldehyde as the cross-linking agent.
34517029	1	3	theme	well-known	177:186	arg1	chitosan					208:215	chitosan	208:215	chitosan	208:215	We present an approach for synthesis of a micro-porous composite of two well-known biopolymers, namely chitosan and alginate, using glutaraldehyde as the cross-linking agent.
34517029	8	4	with	agreement	1342:1350	arg1	those					1357:1361	those	1357:1361	those	1357:1361	Interestingly, the values of the adsorption capacity obtained in column adsorption method are in close agreement with those obtained in batch adsorption experiments, which shows the potential of the synthesized composite for uptake of dyes.
34517029	8	5	theme	dyes	1474:1477	arg1	uptake					1464:1469	uptake	1464:1469	uptake of dyes	1464:1477	Interestingly, the values of the adsorption capacity obtained in column adsorption method are in close agreement with those obtained in batch adsorption experiments, which shows the potential of the synthesized composite for uptake of dyes.
34517029	4	6	dep	dyes	747:750	arg1	V					806:806	Patent Blue V	794:806	Patent Blue V	794:806	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	4	6	dep	dyes	747:750	arg1	dyes					747:750	three dyes	741:750	three dyes namely Brilliant green, Methyl orange and Patent Blue V	741:806	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	4	6	dep	dyes	747:750	arg1	orange					783:788	Methyl orange	776:788	Methyl orange	776:788	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	4	6	dep	dyes	747:750	arg1	Brilliant					759:767	Brilliant green	759:773	Brilliant green	759:773	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	8	7	theme	close	1336:1340	arg1	agreement					1342:1350	close agreement	1336:1350	close agreement with those obtained in batch adsorption experiments	1336:1402	Interestingly, the values of the adsorption capacity obtained in column adsorption method are in close agreement with those obtained in batch adsorption experiments, which shows the potential of the synthesized composite for uptake of dyes.
34517029	8	8	theme	column	1304:1309	arg1	method					1322:1327	column adsorption method	1304:1327	column adsorption method	1304:1327	Interestingly, the values of the adsorption capacity obtained in column adsorption method are in close agreement with those obtained in batch adsorption experiments, which shows the potential of the synthesized composite for uptake of dyes.
34517029	4	9	theme	Blue	801:804	arg1	V					806:806	Patent Blue V	794:806	Patent Blue V	794:806	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	4	9	theme	Blue	801:804	arg1	dyes					747:750	three dyes	741:750	three dyes namely Brilliant green, Methyl orange and Patent Blue V	741:806	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	0	10	link	Glutaraldehyde-cross-linked	0:26	arg1	chitosan-alginate					28:44	Glutaraldehyde-cross-linked chitosan-alginate	0:44	Glutaraldehyde-cross-linked chitosan-alginate	0:44	Glutaraldehyde-cross-linked chitosan-alginate composite for organic dyes removal from aqueous solutions.
34517029	1	11	theme	biopolymers	188:198	arg1	biopolymers					188:198	two well-known biopolymers	173:198	two well-known biopolymers	173:198	We present an approach for synthesis of a micro-porous composite of two well-known biopolymers, namely chitosan and alginate, using glutaraldehyde as the cross-linking agent.
34517029	1	11	theme	biopolymers	188:198	arg1	composite					160:168	a micro-porous composite	145:168	a micro-porous composite of two well-known biopolymers, namely chitosan and alginate	145:228	We present an approach for synthesis of a micro-porous composite of two well-known biopolymers, namely chitosan and alginate, using glutaraldehyde as the cross-linking agent.
34517029	7	12	from	nature	1231:1236	arg1	enthalpy-driven					1196:1210	enthalpy-driven	1196:1210	enthalpy-driven	1196:1210	A thermodynamic study revealed that the process of adsorption was enthalpy-driven and spontaneous in nature.
34517029	7	12	from	nature	1231:1236	arg1	process					1170:1176	the process	1166:1176	the process of adsorption	1166:1190	A thermodynamic study revealed that the process of adsorption was enthalpy-driven and spontaneous in nature.
34517029	7	13	theme	thermodynamic	1132:1144	arg1	study					1146:1150	A thermodynamic study	1130:1150	A thermodynamic study	1130:1150	A thermodynamic study revealed that the process of adsorption was enthalpy-driven and spontaneous in nature.
34517029	6	14	theme	order	1114:1118	arg1	kinetics					1120:1127	pseudo second order kinetics	1100:1127	pseudo second order kinetics	1100:1127	It was found that the adsorption process follows a Freundlich adsorption isotherm and pseudo second order kinetics.
34517029	0	15	theme	Glutaraldehyde-cross-linked	0:26	arg1	chitosan-alginate					28:44	Glutaraldehyde-cross-linked chitosan-alginate	0:44	Glutaraldehyde-cross-linked chitosan-alginate	0:44	Glutaraldehyde-cross-linked chitosan-alginate composite for organic dyes removal from aqueous solutions.
34517029	4	16	theme	cross-linked	597:608	arg1	adsorbent					727:735	adsorbent	727:735	adsorbent	727:735	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	4	16	theme	cross-linked	597:608	arg1	chitosan-alginate					610:626	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite	555:636	chitosan-alginate	610:626	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	3	17	dep	the	438:440	arg1	help					442:445	help	442:445	help	442:445	Chitosan was modified using aniline with the help of formaldehyde crosslinker and then the twizer was further crosslinked with alginate using glutaraldehyde.
34517029	4	18	theme	Methyl	776:781	arg1	dyes					747:750	three dyes	741:750	three dyes namely Brilliant green, Methyl orange and Patent Blue V	741:806	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	4	18	theme	Methyl	776:781	arg1	orange					783:788	Methyl orange	776:788	Methyl orange	776:788	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	4	19	theme	glutaraldehyde	582:595	arg1	adsorbent					727:735	adsorbent	727:735	adsorbent	727:735	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	4	19	theme	glutaraldehyde	582:595	arg1	chitosan-alginate					610:626	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite	555:636	chitosan-alginate	610:626	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	4	20	theme	Patent	794:799	arg1	V					806:806	Patent Blue V	794:806	Patent Blue V	794:806	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	4	20	theme	Patent	794:799	arg1	dyes					747:750	three dyes	741:750	three dyes namely Brilliant green, Methyl orange and Patent Blue V	741:806	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	4	21	theme	spectral	679:686	arg1	techniques					688:697	spectral techniques	679:697	spectral techniques	679:697	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	6	22	theme	second	1107:1112	arg1	kinetics					1120:1127	pseudo second order kinetics	1100:1127	pseudo second order kinetics	1100:1127	It was found that the adsorption process follows a Freundlich adsorption isotherm and pseudo second order kinetics.
34517029	4	23	theme	composite	628:636	arg1	adsorbent					727:735	adsorbent	727:735	adsorbent	727:735	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	4	23	theme	composite	628:636	arg1	chitosan-alginate					610:626	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite	555:636	chitosan-alginate	610:626	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	5	24	theme	monolayer	859:867	arg1	Qmax					890:893	Qmax	890:893	Qmax	890:893	The pHPZC of the material was 7.5 and the maximum monolayer adsorption capacity (Qmax) was found to be 235.82, 198.09 and 117.34 mg g-1 for BG (at pH 8.0), MO (at pH 6.0) and PBV (at pH 3.0) respectively.
34517029	5	24	theme	monolayer	859:867	arg1	capacity					880:887	the maximum monolayer adsorption capacity	847:887	the maximum monolayer adsorption capacity (Qmax)	847:894	The pHPZC of the material was 7.5 and the maximum monolayer adsorption capacity (Qmax) was found to be 235.82, 198.09 and 117.34 mg g-1 for BG (at pH 8.0), MO (at pH 6.0) and PBV (at pH 3.0) respectively.
34517029	5	24	theme	monolayer	859:867	arg1	235.82					912:917	235.82	912:917	235.82	912:917	The pHPZC of the material was 7.5 and the maximum monolayer adsorption capacity (Qmax) was found to be 235.82, 198.09 and 117.34 mg g-1 for BG (at pH 8.0), MO (at pH 6.0) and PBV (at pH 3.0) respectively.
34517029	0	25	theme	dyes	68:71	arg1	removal					73:79	organic dyes removal	60:79	organic dyes removal from aqueous solutions	60:102	Glutaraldehyde-cross-linked chitosan-alginate composite for organic dyes removal from aqueous solutions.
34517029	8	26	theme	capacity	1283:1290	arg1	values					1258:1263	the values	1254:1263	the values of the adsorption capacity obtained in column adsorption method	1254:1327	Interestingly, the values of the adsorption capacity obtained in column adsorption method are in close agreement with those obtained in batch adsorption experiments, which shows the potential of the synthesized composite for uptake of dyes.
34517029	5	27	theme	adsorption	869:878	arg1	Qmax					890:893	Qmax	890:893	Qmax	890:893	The pHPZC of the material was 7.5 and the maximum monolayer adsorption capacity (Qmax) was found to be 235.82, 198.09 and 117.34 mg g-1 for BG (at pH 8.0), MO (at pH 6.0) and PBV (at pH 3.0) respectively.
34517029	5	27	theme	adsorption	869:878	arg1	capacity					880:887	the maximum monolayer adsorption capacity	847:887	the maximum monolayer adsorption capacity (Qmax)	847:894	The pHPZC of the material was 7.5 and the maximum monolayer adsorption capacity (Qmax) was found to be 235.82, 198.09 and 117.34 mg g-1 for BG (at pH 8.0), MO (at pH 6.0) and PBV (at pH 3.0) respectively.
34517029	5	27	theme	adsorption	869:878	arg1	235.82					912:917	235.82	912:917	235.82	912:917	The pHPZC of the material was 7.5 and the maximum monolayer adsorption capacity (Qmax) was found to be 235.82, 198.09 and 117.34 mg g-1 for BG (at pH 8.0), MO (at pH 6.0) and PBV (at pH 3.0) respectively.
34517029	0	28	theme	organic	60:66	arg1	removal					73:79	organic dyes removal	60:79	organic dyes removal from aqueous solutions	60:102	Glutaraldehyde-cross-linked chitosan-alginate composite for organic dyes removal from aqueous solutions.
34517029	4	29	theme	synthesized	559:569	arg1	adsorbent					727:735	adsorbent	727:735	adsorbent	727:735	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	4	29	theme	synthesized	559:569	arg1	chitosan-alginate					610:626	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite	555:636	chitosan-alginate	610:626	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	0	30	from	solutions	94:102	arg1	removal					73:79	organic dyes removal	60:79	organic dyes removal from aqueous solutions	60:102	Glutaraldehyde-cross-linked chitosan-alginate composite for organic dyes removal from aqueous solutions.
34517029	8	31	theme	adsorption	1311:1320	arg1	method					1322:1327	column adsorption method	1304:1327	column adsorption method	1304:1327	Interestingly, the values of the adsorption capacity obtained in column adsorption method are in close agreement with those obtained in batch adsorption experiments, which shows the potential of the synthesized composite for uptake of dyes.
34517029	3	32	theme	formaldehyde	450:461	arg1	crosslinker					463:473	formaldehyde crosslinker	450:473	formaldehyde crosslinker	450:473	Chitosan was modified using aniline with the help of formaldehyde crosslinker and then the twizer was further crosslinked with alginate using glutaraldehyde.
34517029	8	33	theme	batch	1375:1379	arg1	experiments					1392:1402	batch adsorption experiments	1375:1402	batch adsorption experiments	1375:1402	Interestingly, the values of the adsorption capacity obtained in column adsorption method are in close agreement with those obtained in batch adsorption experiments, which shows the potential of the synthesized composite for uptake of dyes.
34517029	5	34	theme	material	826:833	arg1	7.5					839:841	7.5	839:841	7.5	839:841	The pHPZC of the material was 7.5 and the maximum monolayer adsorption capacity (Qmax) was found to be 235.82, 198.09 and 117.34 mg g-1 for BG (at pH 8.0), MO (at pH 6.0) and PBV (at pH 3.0) respectively.
34517029	5	34	theme	material	826:833	arg1	pHPZC					813:817	The pHPZC	809:817	The pHPZC of the material	809:833	The pHPZC of the material was 7.5 and the maximum monolayer adsorption capacity (Qmax) was found to be 235.82, 198.09 and 117.34 mg g-1 for BG (at pH 8.0), MO (at pH 6.0) and PBV (at pH 3.0) respectively.
34517029	8	35	theme	synthesized	1438:1448	arg1	composite					1450:1458	the synthesized composite	1434:1458	the synthesized composite	1434:1458	Interestingly, the values of the adsorption capacity obtained in column adsorption method are in close agreement with those obtained in batch adsorption experiments, which shows the potential of the synthesized composite for uptake of dyes.
34517029	6	36	theme	adsorption	1076:1085	arg1	isotherm					1087:1094	a Freundlich adsorption isotherm	1063:1094	a Freundlich adsorption isotherm	1063:1094	It was found that the adsorption process follows a Freundlich adsorption isotherm and pseudo second order kinetics.
34517029	4	37	theme	composite	571:579	arg1	adsorbent					727:735	adsorbent	727:735	adsorbent	727:735	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	4	37	theme	composite	571:579	arg1	chitosan-alginate					610:626	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite	555:636	chitosan-alginate	610:626	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	6	38	theme	Freundlich	1065:1074	arg1	isotherm					1087:1094	a Freundlich adsorption isotherm	1063:1094	a Freundlich adsorption isotherm	1063:1094	It was found that the adsorption process follows a Freundlich adsorption isotherm and pseudo second order kinetics.
34517029	6	39	theme	pseudo	1100:1105	arg1	kinetics					1120:1127	pseudo second order kinetics	1100:1127	pseudo second order kinetics	1100:1127	It was found that the adsorption process follows a Freundlich adsorption isotherm and pseudo second order kinetics.
34517029	8	40	theme	adsorption	1381:1390	arg1	experiments					1392:1402	batch adsorption experiments	1375:1402	batch adsorption experiments	1375:1402	Interestingly, the values of the adsorption capacity obtained in column adsorption method are in close agreement with those obtained in batch adsorption experiments, which shows the potential of the synthesized composite for uptake of dyes.
34517029	1	41	theme	micro-porous	147:158	arg1	biopolymers					188:198	two well-known biopolymers	173:198	two well-known biopolymers	173:198	We present an approach for synthesis of a micro-porous composite of two well-known biopolymers, namely chitosan and alginate, using glutaraldehyde as the cross-linking agent.
34517029	1	41	theme	micro-porous	147:158	arg1	composite					160:168	a micro-porous composite	145:168	a micro-porous composite of two well-known biopolymers, namely chitosan and alginate	145:228	We present an approach for synthesis of a micro-porous composite of two well-known biopolymers, namely chitosan and alginate, using glutaraldehyde as the cross-linking agent.
34517029	4	42	theme	-G-Al	648:652	arg1	adsorbent					727:735	adsorbent	727:735	adsorbent	727:735	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	4	42	theme	-G-Al	648:652	arg1	chitosan-alginate					610:626	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite	555:636	chitosan-alginate	610:626	The synthesized composite, glutaraldehyde cross-linked chitosan-alginate composite [(Cs-F-An)-G-Al] was characterized using spectral techniques and employed as a potential adsorbent for three dyes namely Brilliant green, Methyl orange and Patent Blue V.
34517029	7	43	theme	adsorption	1181:1190	arg1	enthalpy-driven					1196:1210	enthalpy-driven	1196:1210	enthalpy-driven	1196:1210	A thermodynamic study revealed that the process of adsorption was enthalpy-driven and spontaneous in nature.
34517029	7	43	theme	adsorption	1181:1190	arg1	process					1170:1176	the process	1166:1176	the process of adsorption	1166:1190	A thermodynamic study revealed that the process of adsorption was enthalpy-driven and spontaneous in nature.
34517029	8	44	theme	composite	1450:1458	arg1	potential					1421:1429	the potential	1417:1429	the potential of the synthesized composite for uptake of dyes	1417:1477	Interestingly, the values of the adsorption capacity obtained in column adsorption method are in close agreement with those obtained in batch adsorption experiments, which shows the potential of the synthesized composite for uptake of dyes.
34517029	5	45	theme	maximum	851:857	arg1	Qmax					890:893	Qmax	890:893	Qmax	890:893	The pHPZC of the material was 7.5 and the maximum monolayer adsorption capacity (Qmax) was found to be 235.82, 198.09 and 117.34 mg g-1 for BG (at pH 8.0), MO (at pH 6.0) and PBV (at pH 3.0) respectively.
34517029	5	45	theme	maximum	851:857	arg1	capacity					880:887	the maximum monolayer adsorption capacity	847:887	the maximum monolayer adsorption capacity (Qmax)	847:894	The pHPZC of the material was 7.5 and the maximum monolayer adsorption capacity (Qmax) was found to be 235.82, 198.09 and 117.34 mg g-1 for BG (at pH 8.0), MO (at pH 6.0) and PBV (at pH 3.0) respectively.
34517029	5	45	theme	maximum	851:857	arg1	235.82					912:917	235.82	912:917	235.82	912:917	The pHPZC of the material was 7.5 and the maximum monolayer adsorption capacity (Qmax) was found to be 235.82, 198.09 and 117.34 mg g-1 for BG (at pH 8.0), MO (at pH 6.0) and PBV (at pH 3.0) respectively.
34517029	1	46	theme	composite	160:168	arg1	synthesis					132:140	synthesis	132:140	synthesis of a micro-porous composite of two well-known biopolymers, namely chitosan and alginate	132:228	We present an approach for synthesis of a micro-porous composite of two well-known biopolymers, namely chitosan and alginate, using glutaraldehyde as the cross-linking agent.
34517029	0	47	theme	aqueous	86:92	arg1	solutions					94:102	aqueous solutions	86:102	aqueous solutions	86:102	Glutaraldehyde-cross-linked chitosan-alginate composite for organic dyes removal from aqueous solutions.
34517029	8	48	theme	adsorption	1272:1281	arg1	capacity					1283:1290	the adsorption capacity	1268:1290	the adsorption capacity obtained in column adsorption method	1268:1327	Interestingly, the values of the adsorption capacity obtained in column adsorption method are in close agreement with those obtained in batch adsorption experiments, which shows the potential of the synthesized composite for uptake of dyes.
34517029	1	49	theme	cross-linking	259:271	arg1	glutaraldehyde					237:250	glutaraldehyde	237:250	glutaraldehyde	237:250	We present an approach for synthesis of a micro-porous composite of two well-known biopolymers, namely chitosan and alginate, using glutaraldehyde as the cross-linking agent.
34517029	1	49	theme	cross-linking	259:271	arg1	agent					273:277	the cross-linking agent	255:277	the cross-linking agent	255:277	We present an approach for synthesis of a micro-porous composite of two well-known biopolymers, namely chitosan and alginate, using glutaraldehyde as the cross-linking agent.
32827799	0	0	theme	vessel	108:113	arg1	graft					115:119	small-caliber artificial blood vessel graft	77:119	small-caliber artificial blood vessel graft	77:119	Construction and performance evaluation of Hep/silk-PLCL composite nanofiber small-caliber artificial blood vessel graft.
32827799	10	1	theme	vascular	1538:1545	arg1	tissue					1547:1552	autogenous vascular tissue	1527:1552	autogenous vascular tissue	1527:1552	In addition, the regenerated vascular tissue provided a similar function to that of autogenous vascular tissue when the graft was highly degraded.
32827799	6	2	theme	mechanical	868:877	arg1	support					879:885	mechanical support	868:885	mechanical support	868:885	The core PLCL nanofibers provided mechanical support during vascular reconstruction, and the shell heparin/silk gel layer enhanced the biocompatibility of the grafts.
32827799	1	3	theme	blood	176:180	arg1	grafts					189:194	small-caliber blood vessel grafts	162:194	small-caliber blood vessel grafts	162:194	To meet the growing clinical demand for small-caliber blood vessel grafts to treat cardiovascular diseases, it is necessary to develop safe and long-term unobstructed grafts.
32827799	6	4	theme	vascular	894:901	arg1	reconstruction					903:916	vascular reconstruction	894:916	vascular reconstruction	894:916	The core PLCL nanofibers provided mechanical support during vascular reconstruction, and the shell heparin/silk gel layer enhanced the biocompatibility of the grafts.
32827799	0	5	theme	blood	102:106	arg1	graft					115:119	small-caliber artificial blood vessel graft	77:119	small-caliber artificial blood vessel graft	77:119	Construction and performance evaluation of Hep/silk-PLCL composite nanofiber small-caliber artificial blood vessel graft.
32827799	1	6	theme	vessel	182:187	arg1	grafts					189:194	small-caliber blood vessel grafts	162:194	small-caliber blood vessel grafts	162:194	To meet the growing clinical demand for small-caliber blood vessel grafts to treat cardiovascular diseases, it is necessary to develop safe and long-term unobstructed grafts.
32827799	1	7	theme	long-term	266:274	arg1	grafts					289:294	safe and long-term unobstructed grafts	257:294	safe and long-term unobstructed grafts	257:294	To meet the growing clinical demand for small-caliber blood vessel grafts to treat cardiovascular diseases, it is necessary to develop safe and long-term unobstructed grafts.
32827799	5	8	theme	synthetic	715:723	arg1	materials					725:733	synthetic materials	715:733	synthetic materials	715:733	This special structure in which the core layer was made of synthetic materials and the shell layer was made of natural materials took advantage of these two different materials.
32827799	5	9	theme	special	661:667	arg1	structure					669:677	This special structure	656:677	This special structure in which the core layer was made of synthetic materials and the shell layer was made of natural materials	656:783	This special structure in which the core layer was made of synthetic materials and the shell layer was made of natural materials took advantage of these two different materials.
32827799	11	10	theme	proposed	1600:1607	arg1	method					1609:1614	the proposed method	1596:1614	the proposed method	1596:1614	Thus, the proposed method produced a graft that could maintain long-term patency in vivo and remodel vascular tissue successfully.
32827799	1	11	theme	unobstructed	276:287	arg1	grafts					289:294	safe and long-term unobstructed grafts	257:294	safe and long-term unobstructed grafts	257:294	To meet the growing clinical demand for small-caliber blood vessel grafts to treat cardiovascular diseases, it is necessary to develop safe and long-term unobstructed grafts.
32827799	4	12	theme	core	511:514	arg1	fiber					516:520	The core fiber	507:520	The core fiber	507:520	The core fiber was poly(l-lactide-co-caprolactone) (PLCL)-based and the core fibers were coated with heparin/silk gel, which acted as a shell layer.
32827799	4	12	theme	core	511:514	arg1	-based					564:569	-based	564:569	-based	564:569	The core fiber was poly(l-lactide-co-caprolactone) (PLCL)-based and the core fibers were coated with heparin/silk gel, which acted as a shell layer.
32827799	4	12	theme	core	511:514	arg1	poly					526:529	poly	526:529	poly(l-lactide-co-caprolactone)	526:556	The core fiber was poly(l-lactide-co-caprolactone) (PLCL)-based and the core fibers were coated with heparin/silk gel, which acted as a shell layer.
32827799	8	13	theme	transplantation	1327:1341	arg1	stage					1318:1322	the late stage	1309:1322	the late stage of transplantation	1309:1341	All of the graft materials were biodegradable and safe biomaterials, and the degradation of the graft provided space for the growth of regenerated tissue in the late stage of transplantation.
32827799	10	14	theme	vascular	1472:1479	arg1	tissue					1481:1486	the regenerated vascular tissue	1456:1486	the regenerated vascular tissue	1456:1486	In addition, the regenerated vascular tissue provided a similar function to that of autogenous vascular tissue when the graft was highly degraded.
32827799	8	15	theme	safe	1202:1205	arg1	biomaterials					1207:1218	biodegradable and safe biomaterials	1184:1218	biodegradable and safe biomaterials	1184:1218	All of the graft materials were biodegradable and safe biomaterials, and the degradation of the graft provided space for the growth of regenerated tissue in the late stage of transplantation.
32827799	3	16	theme	conjugate	454:462	arg1	electrospinning					464:478	conjugate electrospinning	454:478	conjugate electrospinning	454:478	A composite nanofiber core-shell structure was prepared by a combination of conjugate electrospinning and freeze-dry technology.
32827799	10	17	theme	regenerated	1460:1470	arg1	tissue					1481:1486	the regenerated vascular tissue	1456:1486	the regenerated vascular tissue	1456:1486	In addition, the regenerated vascular tissue provided a similar function to that of autogenous vascular tissue when the graft was highly degraded.
32827799	3	18	theme	electrospinning	464:478	arg1	combination					439:449	a combination	437:449	a combination of conjugate electrospinning and freeze-dry technology	437:504	A composite nanofiber core-shell structure was prepared by a combination of conjugate electrospinning and freeze-dry technology.
32827799	3	19	theme	core-shell	400:409	arg1	structure					411:419	A composite nanofiber core-shell structure	378:419	A composite nanofiber core-shell structure	378:419	A composite nanofiber core-shell structure was prepared by a combination of conjugate electrospinning and freeze-dry technology.
32827799	4	20	theme	core	579:582	arg1	fibers					584:589	the core fibers	575:589	the core fibers	575:589	The core fiber was poly(l-lactide-co-caprolactone) (PLCL)-based and the core fibers were coated with heparin/silk gel, which acted as a shell layer.
32827799	5	21	theme	shell	743:747	arg1	layer					749:753	the shell layer	739:753	the shell layer	739:753	This special structure in which the core layer was made of synthetic materials and the shell layer was made of natural materials took advantage of these two different materials.
32827799	8	22	theme	biodegradable	1184:1196	arg1	biomaterials					1207:1218	biodegradable and safe biomaterials	1184:1218	biodegradable and safe biomaterials	1184:1218	All of the graft materials were biodegradable and safe biomaterials, and the degradation of the graft provided space for the growth of regenerated tissue in the late stage of transplantation.
32827799	2	23	theme	biodegradable	314:326	arg1	graft					328:332	a biodegradable graft	312:332	a biodegradable graft made of composite nanofibers	312:361	In this study, a biodegradable graft made of composite nanofibers is introduced.
32827799	1	24	theme	cardiovascular	205:218	arg1	diseases					220:227	cardiovascular diseases	205:227	cardiovascular diseases	205:227	To meet the growing clinical demand for small-caliber blood vessel grafts to treat cardiovascular diseases, it is necessary to develop safe and long-term unobstructed grafts.
32827799	3	25	theme	composite	380:388	arg1	structure					411:419	A composite nanofiber core-shell structure	378:419	A composite nanofiber core-shell structure	378:419	A composite nanofiber core-shell structure was prepared by a combination of conjugate electrospinning and freeze-dry technology.
32827799	11	26	theme	long-term	1653:1661	arg1	patency					1663:1669	long-term patency	1653:1669	long-term patency	1653:1669	Thus, the proposed method produced a graft that could maintain long-term patency in vivo and remodel vascular tissue successfully.
32827799	10	27	theme	similar	1499:1505	arg1	function					1507:1514	a similar function	1497:1514	a similar function	1497:1514	In addition, the regenerated vascular tissue provided a similar function to that of autogenous vascular tissue when the graft was highly degraded.
32827799	3	28	theme	technology	495:504	arg1	combination					439:449	a combination	437:449	a combination of conjugate electrospinning and freeze-dry technology	437:504	A composite nanofiber core-shell structure was prepared by a combination of conjugate electrospinning and freeze-dry technology.
32827799	3	29	theme	nanofiber	390:398	arg1	structure					411:419	A composite nanofiber core-shell structure	378:419	A composite nanofiber core-shell structure	378:419	A composite nanofiber core-shell structure was prepared by a combination of conjugate electrospinning and freeze-dry technology.
32827799	9	30	theme	Animal	1344:1349	arg1	experiments					1351:1361	Animal experiments	1344:1361	Animal experiments	1344:1361	Animal experiments showed that the graft remained unobstructed for more than eight months in vivo.
32827799	8	31	theme	graft	1163:1167	arg1	materials					1169:1177	the graft materials	1159:1177	the graft materials	1159:1177	All of the graft materials were biodegradable and safe biomaterials, and the degradation of the graft provided space for the growth of regenerated tissue in the late stage of transplantation.
32827799	5	32	theme	materials	823:831	arg1	advantage					790:798	advantage	790:798	advantage of these two different materials	790:831	This special structure in which the core layer was made of synthetic materials and the shell layer was made of natural materials took advantage of these two different materials.
32827799	0	33	theme	Hep/silk-PLCL	43:55	arg1	composite					57:65	Hep/silk-PLCL composite	43:65	Hep/silk-PLCL composite	43:65	Construction and performance evaluation of Hep/silk-PLCL composite nanofiber small-caliber artificial blood vessel graft.
32827799	2	34	theme	composite	342:350	arg1	nanofibers					352:361	composite nanofibers	342:361	composite nanofibers	342:361	In this study, a biodegradable graft made of composite nanofibers is introduced.
32827799	11	35	theme	vascular	1691:1698	arg1	tissue					1700:1705	vascular tissue	1691:1705	vascular tissue	1691:1705	Thus, the proposed method produced a graft that could maintain long-term patency in vivo and remodel vascular tissue successfully.
32827799	8	36	theme	graft	1248:1252	arg1	degradation					1229:1239	the degradation	1225:1239	the degradation of the graft	1225:1252	All of the graft materials were biodegradable and safe biomaterials, and the degradation of the graft provided space for the growth of regenerated tissue in the late stage of transplantation.
32827799	7	37	from	release	1015:1021	arg1	stage					1047:1051	the early stage	1037:1051	the early stage after transplantation	1037:1073	Moreover, the release of heparin in the early stage after transplantation could regulate the microenvironment and inhibit the proliferation of intima.
32827799	5	38	theme	natural	767:773	arg1	materials					775:783	natural materials	767:783	natural materials	767:783	This special structure in which the core layer was made of synthetic materials and the shell layer was made of natural materials took advantage of these two different materials.
32827799	6	39	theme	PLCL	843:846	arg1	nanofibers					848:857	The core PLCL nanofibers	834:857	The core PLCL nanofibers	834:857	The core PLCL nanofibers provided mechanical support during vascular reconstruction, and the shell heparin/silk gel layer enhanced the biocompatibility of the grafts.
32827799	3	40	theme	freeze-dry	484:493	arg1	technology					495:504	freeze-dry technology	484:504	freeze-dry technology	484:504	A composite nanofiber core-shell structure was prepared by a combination of conjugate electrospinning and freeze-dry technology.
32827799	7	41	theme	intima	1144:1149	arg1	proliferation					1127:1139	the proliferation	1123:1139	the proliferation of intima	1123:1149	Moreover, the release of heparin in the early stage after transplantation could regulate the microenvironment and inhibit the proliferation of intima.
32827799	6	42	theme	gel	946:948	arg1	layer					950:954	the shell heparin/silk gel layer	923:954	the shell heparin/silk gel layer	923:954	The core PLCL nanofibers provided mechanical support during vascular reconstruction, and the shell heparin/silk gel layer enhanced the biocompatibility of the grafts.
32827799	6	43	theme	core	838:841	arg1	nanofibers					848:857	The core PLCL nanofibers	834:857	The core PLCL nanofibers	834:857	The core PLCL nanofibers provided mechanical support during vascular reconstruction, and the shell heparin/silk gel layer enhanced the biocompatibility of the grafts.
32827799	8	44	theme	regenerated	1287:1297	arg1	tissue					1299:1304	regenerated tissue	1287:1304	regenerated tissue	1287:1304	All of the graft materials were biodegradable and safe biomaterials, and the degradation of the graft provided space for the growth of regenerated tissue in the late stage of transplantation.
32827799	0	45	theme	composite	57:65	arg1	Construction					0:11	Construction	0:11	Construction	0:11	Construction and performance evaluation of Hep/silk-PLCL composite nanofiber small-caliber artificial blood vessel graft.
32827799	0	45	theme	composite	57:65	arg1	evaluation					29:38	performance evaluation	17:38	performance evaluation	17:38	Construction and performance evaluation of Hep/silk-PLCL composite nanofiber small-caliber artificial blood vessel graft.
32827799	6	46	theme	heparin/silk	933:944	arg1	layer					950:954	the shell heparin/silk gel layer	923:954	the shell heparin/silk gel layer	923:954	The core PLCL nanofibers provided mechanical support during vascular reconstruction, and the shell heparin/silk gel layer enhanced the biocompatibility of the grafts.
32827799	7	47	theme	early	1041:1045	arg1	stage					1047:1051	the early stage	1037:1051	the early stage after transplantation	1037:1073	Moreover, the release of heparin in the early stage after transplantation could regulate the microenvironment and inhibit the proliferation of intima.
32827799	4	48	theme	heparin/silk	608:619	arg1	layer					649:653	a shell layer	641:653	a shell layer	641:653	The core fiber was poly(l-lactide-co-caprolactone) (PLCL)-based and the core fibers were coated with heparin/silk gel, which acted as a shell layer.
32827799	4	48	theme	heparin/silk	608:619	arg1	gel					621:623	heparin/silk gel	608:623	heparin/silk gel	608:623	The core fiber was poly(l-lactide-co-caprolactone) (PLCL)-based and the core fibers were coated with heparin/silk gel, which acted as a shell layer.
32827799	1	49	theme	growing	134:140	arg1	demand					151:156	the growing clinical demand	130:156	the growing clinical demand for small-caliber blood vessel grafts to treat cardiovascular diseases	130:227	To meet the growing clinical demand for small-caliber blood vessel grafts to treat cardiovascular diseases, it is necessary to develop safe and long-term unobstructed grafts.
32827799	6	50	theme	shell	927:931	arg1	layer					950:954	the shell heparin/silk gel layer	923:954	the shell heparin/silk gel layer	923:954	The core PLCL nanofibers provided mechanical support during vascular reconstruction, and the shell heparin/silk gel layer enhanced the biocompatibility of the grafts.
32827799	7	51	theme	heparin	1026:1032	arg1	release					1015:1021	the release	1011:1021	the release of heparin in the early stage after transplantation	1011:1073	Moreover, the release of heparin in the early stage after transplantation could regulate the microenvironment and inhibit the proliferation of intima.
32827799	1	52	theme	clinical	142:149	arg1	demand					151:156	the growing clinical demand	130:156	the growing clinical demand for small-caliber blood vessel grafts to treat cardiovascular diseases	130:227	To meet the growing clinical demand for small-caliber blood vessel grafts to treat cardiovascular diseases, it is necessary to develop safe and long-term unobstructed grafts.
32827799	6	53	theme	grafts	993:998	arg1	biocompatibility					969:984	the biocompatibility	965:984	the biocompatibility of the grafts	965:998	The core PLCL nanofibers provided mechanical support during vascular reconstruction, and the shell heparin/silk gel layer enhanced the biocompatibility of the grafts.
32827799	5	54	theme	core	692:695	arg1	layer					697:701	the core layer	688:701	the core layer	688:701	This special structure in which the core layer was made of synthetic materials and the shell layer was made of natural materials took advantage of these two different materials.
32827799	8	55	from	growth	1277:1282	arg1	stage					1318:1322	the late stage	1309:1322	the late stage of transplantation	1309:1341	All of the graft materials were biodegradable and safe biomaterials, and the degradation of the graft provided space for the growth of regenerated tissue in the late stage of transplantation.
32827799	8	56	theme	tissue	1299:1304	arg1	growth					1277:1282	the growth	1273:1282	the growth of regenerated tissue in the late stage of transplantation	1273:1341	All of the graft materials were biodegradable and safe biomaterials, and the degradation of the graft provided space for the growth of regenerated tissue in the late stage of transplantation.
32827799	0	57	theme	artificial	91:100	arg1	graft					115:119	small-caliber artificial blood vessel graft	77:119	small-caliber artificial blood vessel graft	77:119	Construction and performance evaluation of Hep/silk-PLCL composite nanofiber small-caliber artificial blood vessel graft.
32827799	4	58	theme	shell	643:647	arg1	layer					649:653	a shell layer	641:653	a shell layer	641:653	The core fiber was poly(l-lactide-co-caprolactone) (PLCL)-based and the core fibers were coated with heparin/silk gel, which acted as a shell layer.
32827799	4	58	theme	shell	643:647	arg1	gel					621:623	heparin/silk gel	608:623	heparin/silk gel	608:623	The core fiber was poly(l-lactide-co-caprolactone) (PLCL)-based and the core fibers were coated with heparin/silk gel, which acted as a shell layer.
32827799	5	59	theme	different	813:821	arg1	materials					823:831	these two different materials	803:831	these two different materials	803:831	This special structure in which the core layer was made of synthetic materials and the shell layer was made of natural materials took advantage of these two different materials.
32827799	8	60	theme	late	1313:1316	arg1	stage					1318:1322	the late stage	1309:1322	the late stage of transplantation	1309:1341	All of the graft materials were biodegradable and safe biomaterials, and the degradation of the graft provided space for the growth of regenerated tissue in the late stage of transplantation.
32827799	10	61	theme	autogenous	1527:1536	arg1	tissue					1547:1552	autogenous vascular tissue	1527:1552	autogenous vascular tissue	1527:1552	In addition, the regenerated vascular tissue provided a similar function to that of autogenous vascular tissue when the graft was highly degraded.
32827799	0	62	theme	small-caliber	77:89	arg1	graft					115:119	small-caliber artificial blood vessel graft	77:119	small-caliber artificial blood vessel graft	77:119	Construction and performance evaluation of Hep/silk-PLCL composite nanofiber small-caliber artificial blood vessel graft.
32827799	4	63	dep	-based	564:569	arg1	fiber					516:520	The core fiber	507:520	The core fiber	507:520	The core fiber was poly(l-lactide-co-caprolactone) (PLCL)-based and the core fibers were coated with heparin/silk gel, which acted as a shell layer.
32827799	4	63	dep	-based	564:569	arg1	-based					564:569	-based	564:569	-based	564:569	The core fiber was poly(l-lactide-co-caprolactone) (PLCL)-based and the core fibers were coated with heparin/silk gel, which acted as a shell layer.
32827799	4	63	dep	-based	564:569	arg1	l-lactide-co-caprolactone					531:555	l-lactide-co-caprolactone	531:555	l-lactide-co-caprolactone	531:555	The core fiber was poly(l-lactide-co-caprolactone) (PLCL)-based and the core fibers were coated with heparin/silk gel, which acted as a shell layer.
32827799	4	63	dep	-based	564:569	arg1	poly					526:529	poly	526:529	poly(l-lactide-co-caprolactone)	526:556	The core fiber was poly(l-lactide-co-caprolactone) (PLCL)-based and the core fibers were coated with heparin/silk gel, which acted as a shell layer.
32827799	0	64	theme	performance	17:27	arg1	evaluation					29:38	performance evaluation	17:38	performance evaluation	17:38	Construction and performance evaluation of Hep/silk-PLCL composite nanofiber small-caliber artificial blood vessel graft.
32827799	1	65	theme	small-caliber	162:174	arg1	grafts					189:194	small-caliber blood vessel grafts	162:194	small-caliber blood vessel grafts	162:194	To meet the growing clinical demand for small-caliber blood vessel grafts to treat cardiovascular diseases, it is necessary to develop safe and long-term unobstructed grafts.
32827799	1	66	theme	safe	257:260	arg1	grafts					289:294	safe and long-term unobstructed grafts	257:294	safe and long-term unobstructed grafts	257:294	To meet the growing clinical demand for small-caliber blood vessel grafts to treat cardiovascular diseases, it is necessary to develop safe and long-term unobstructed grafts.
32845257	7	0	theme	g-1	1035:1037	arg1	g-1					1053:1055	0.08-0.13 mg g-1, 0.04-0.08 mg g-1	1022:1055	0.08-0.13 mg g-1, 0.04-0.08 mg g-1	1022:1055	The phytate, tannin, oxalate and saponin contents ranged from 0.08-0.13 mg g-1, 0.04-0.08 mg g-1, 0.21-0.31 mg g-1 and 0.18-0.26 mg g-1 respectively.
32845257	2	1	theme	underutilized	248:260	arg1	source					278:283	an underutilized wild yam starch source	245:283	an underutilized wild yam starch source	245:283	Dioscorea villosa is an underutilized wild yam starch source and its starch was isolated and succinylated at 0, 3, 6, 9 and 12% (0-12% succinic anhydride: 88-100% starch) using standard procedures.
32845257	2	1	theme	underutilized	248:260	arg1	villosa					234:240	Dioscorea villosa	224:240	Dioscorea villosa	224:240	Dioscorea villosa is an underutilized wild yam starch source and its starch was isolated and succinylated at 0, 3, 6, 9 and 12% (0-12% succinic anhydride: 88-100% starch) using standard procedures.
32845257	5	2	dep	percentage	711:720	arg1	content					731:737	succinyl content	722:737	succinyl content	722:737	RESULTS The percentage succinyl content and degree of substitution increased from 0-10.45% and 0-0.19% respectively.
32845257	10	3	theme	best	1524:1527	arg1	properties					1552:1561	functional and pasting properties	1529:1561	the best functional and pasting properties	1520:1561	Succinylation at 9% had the best functional and pasting properties, therefore making it suitable as a potential emulsifier, fat replacer to prevent obesity and stabilizer in food applications.
32845257	7	4	theme	0.08-0.13	1022:1030	arg1	mg					1032:1033	mg	1032:1033	mg	1032:1033	The phytate, tannin, oxalate and saponin contents ranged from 0.08-0.13 mg g-1, 0.04-0.08 mg g-1, 0.21-0.31 mg g-1 and 0.18-0.26 mg g-1 respectively.
32845257	10	5	theme	fat	1620:1622	arg1	emulsifier					1608:1617	a potential emulsifier	1596:1617	a potential emulsifier	1596:1617	Succinylation at 9% had the best functional and pasting properties, therefore making it suitable as a potential emulsifier, fat replacer to prevent obesity and stabilizer in food applications.
32845257	10	5	theme	fat	1620:1622	arg1	replacer					1624:1631	fat replacer	1620:1631	fat replacer to prevent obesity and stabilizer in food applications	1620:1686	Succinylation at 9% had the best functional and pasting properties, therefore making it suitable as a potential emulsifier, fat replacer to prevent obesity and stabilizer in food applications.
32845257	10	6	from	%	1514:1514	arg1	Succinylation					1496:1508	Succinylation	1496:1508	Succinylation at 9%	1496:1514	Succinylation at 9% had the best functional and pasting properties, therefore making it suitable as a potential emulsifier, fat replacer to prevent obesity and stabilizer in food applications.
32845257	5	7	dep	RESULTS	699:705	arg1	increased					766:774	increased	766:774	increased from 0-10.45% and 0-0.19% respectively	766:813	RESULTS The percentage succinyl content and degree of substitution increased from 0-10.45% and 0-0.19% respectively.
32845257	3	8	theme	functional	472:481	arg1	substitution					436:447	substitution	436:447	substitution	436:447	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	3	8	theme	functional	472:481	arg1	properties					483:492	functional properties	472:492	functional properties	472:492	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	1	9	theme	food	158:161	arg1	applications					163:174	food applications	158:174	food applications	158:174	BACKGROUND Recent increase in demand for starch in food applications has called for research into other new sources.
32845257	8	10	theme	final	1150:1154	arg1	viscosity					1119:1127	The peak viscosity	1110:1127	The peak viscosity	1110:1127	The peak viscosity, trough, break down, final viscosity, set back, peak time and pasting temperature values ranged from 94.32-274.69 RVU, 83.50-197.50 RVU, 7.94-81.28 RVU, 113.69-367.39 RVU, 30.14-173.89 RVU, 5.30-6.94 min, and 84.78-94.70 °C respectively.
32845257	8	10	theme	final	1150:1154	arg1	viscosity					1156:1164	final viscosity	1150:1164	final viscosity	1150:1164	The peak viscosity, trough, break down, final viscosity, set back, peak time and pasting temperature values ranged from 94.32-274.69 RVU, 83.50-197.50 RVU, 7.94-81.28 RVU, 113.69-367.39 RVU, 30.14-173.89 RVU, 5.30-6.94 min, and 84.78-94.70 °C respectively.
32845257	7	11	theme	saponin	993:999	arg1	contents					1001:1008	The phytate, tannin, oxalate and saponin contents	960:1008	The phytate, tannin, oxalate and saponin contents	960:1008	The phytate, tannin, oxalate and saponin contents ranged from 0.08-0.13 mg g-1, 0.04-0.08 mg g-1, 0.21-0.31 mg g-1 and 0.18-0.26 mg g-1 respectively.
32845257	2	12	theme	yam	267:269	arg1	source					278:283	an underutilized wild yam starch source	245:283	an underutilized wild yam starch source	245:283	Dioscorea villosa is an underutilized wild yam starch source and its starch was isolated and succinylated at 0, 3, 6, 9 and 12% (0-12% succinic anhydride: 88-100% starch) using standard procedures.
32845257	2	12	theme	yam	267:269	arg1	villosa					234:240	Dioscorea villosa	224:240	Dioscorea villosa	224:240	Dioscorea villosa is an underutilized wild yam starch source and its starch was isolated and succinylated at 0, 3, 6, 9 and 12% (0-12% succinic anhydride: 88-100% starch) using standard procedures.
32845257	8	13	theme	peak	1114:1117	arg1	viscosity					1156:1164	final viscosity	1150:1164	final viscosity	1150:1164	The peak viscosity, trough, break down, final viscosity, set back, peak time and pasting temperature values ranged from 94.32-274.69 RVU, 83.50-197.50 RVU, 7.94-81.28 RVU, 113.69-367.39 RVU, 30.14-173.89 RVU, 5.30-6.94 min, and 84.78-94.70 °C respectively.
32845257	8	13	theme	peak	1114:1117	arg1	trough					1130:1135	trough	1130:1135	trough	1130:1135	The peak viscosity, trough, break down, final viscosity, set back, peak time and pasting temperature values ranged from 94.32-274.69 RVU, 83.50-197.50 RVU, 7.94-81.28 RVU, 113.69-367.39 RVU, 30.14-173.89 RVU, 5.30-6.94 min, and 84.78-94.70 °C respectively.
32845257	8	13	theme	peak	1114:1117	arg1	down					1144:1147	break down	1138:1147	break down	1138:1147	The peak viscosity, trough, break down, final viscosity, set back, peak time and pasting temperature values ranged from 94.32-274.69 RVU, 83.50-197.50 RVU, 7.94-81.28 RVU, 113.69-367.39 RVU, 30.14-173.89 RVU, 5.30-6.94 min, and 84.78-94.70 °C respectively.
32845257	8	13	theme	peak	1114:1117	arg1	viscosity					1119:1127	The peak viscosity	1110:1127	The peak viscosity	1110:1127	The peak viscosity, trough, break down, final viscosity, set back, peak time and pasting temperature values ranged from 94.32-274.69 RVU, 83.50-197.50 RVU, 7.94-81.28 RVU, 113.69-367.39 RVU, 30.14-173.89 RVU, 5.30-6.94 min, and 84.78-94.70 °C respectively.
32845257	7	14	theme	oxalate	981:987	arg1	contents					1001:1008	The phytate, tannin, oxalate and saponin contents	960:1008	The phytate, tannin, oxalate and saponin contents	960:1008	The phytate, tannin, oxalate and saponin contents ranged from 0.08-0.13 mg g-1, 0.04-0.08 mg g-1, 0.21-0.31 mg g-1 and 0.18-0.26 mg g-1 respectively.
32845257	11	15	theme	limited	1693:1699	arg1	activities					1708:1717	Its limited enzyme activities	1689:1717	Its limited enzyme activities	1689:1717	Its limited enzyme activities make it desirable in the production of slowly digestible food products, such as snacks for diabetic patients.
32845257	7	16	theme	tannin	973:978	arg1	contents					1001:1008	The phytate, tannin, oxalate and saponin contents	960:1008	The phytate, tannin, oxalate and saponin contents	960:1008	The phytate, tannin, oxalate and saponin contents ranged from 0.08-0.13 mg g-1, 0.04-0.08 mg g-1, 0.21-0.31 mg g-1 and 0.18-0.26 mg g-1 respectively.
32845257	10	17	theme	food	1670:1673	arg1	applications					1675:1686	food applications	1670:1686	food applications	1670:1686	Succinylation at 9% had the best functional and pasting properties, therefore making it suitable as a potential emulsifier, fat replacer to prevent obesity and stabilizer in food applications.
32845257	7	18	theme	g-1	1053:1055	arg1	g-1					1092:1094	0.08-0.13 mg g-1, 0.04-0.08 mg g-1, 0.21-0.31 mg g-1 and 0.18-0.26 mg g-1	1022:1094	0.08-0.13 mg g-1, 0.04-0.08 mg g-1, 0.21-0.31 mg g-1 and 0.18-0.26 mg g-1 respectively	1022:1107	The phytate, tannin, oxalate and saponin contents ranged from 0.08-0.13 mg g-1, 0.04-0.08 mg g-1, 0.21-0.31 mg g-1 and 0.18-0.26 mg g-1 respectively.
32845257	8	19	theme	peak	1177:1180	arg1	time					1182:1185	peak time and pasting temperature values	1177:1216	time	1182:1185	The peak viscosity, trough, break down, final viscosity, set back, peak time and pasting temperature values ranged from 94.32-274.69 RVU, 83.50-197.50 RVU, 7.94-81.28 RVU, 113.69-367.39 RVU, 30.14-173.89 RVU, 5.30-6.94 min, and 84.78-94.70 °C respectively.
32845257	7	20	theme	phytate	964:970	arg1	contents					1001:1008	The phytate, tannin, oxalate and saponin contents	960:1008	The phytate, tannin, oxalate and saponin contents	960:1008	The phytate, tannin, oxalate and saponin contents ranged from 0.08-0.13 mg g-1, 0.04-0.08 mg g-1, 0.21-0.31 mg g-1 and 0.18-0.26 mg g-1 respectively.
32845257	2	21	theme	Dioscorea	224:232	arg1	source					278:283	an underutilized wild yam starch source	245:283	an underutilized wild yam starch source	245:283	Dioscorea villosa is an underutilized wild yam starch source and its starch was isolated and succinylated at 0, 3, 6, 9 and 12% (0-12% succinic anhydride: 88-100% starch) using standard procedures.
32845257	2	21	theme	Dioscorea	224:232	arg1	villosa					234:240	Dioscorea villosa	224:240	Dioscorea villosa	224:240	Dioscorea villosa is an underutilized wild yam starch source and its starch was isolated and succinylated at 0, 3, 6, 9 and 12% (0-12% succinic anhydride: 88-100% starch) using standard procedures.
32845257	1	22	theme	BACKGROUND	107:116	arg1	increase					125:132	BACKGROUND Recent increase	107:132	BACKGROUND Recent increase in demand for starch in food applications	107:174	BACKGROUND Recent increase in demand for starch in food applications has called for research into other new sources.
32845257	10	23	dep	replacer	1624:1631	arg1	prevent					1636:1642	prevent	1636:1642	to prevent obesity and stabilizer in food applications	1633:1686	Succinylation at 9% had the best functional and pasting properties, therefore making it suitable as a potential emulsifier, fat replacer to prevent obesity and stabilizer in food applications.
32845257	3	24	theme	substitution	436:447	arg1	degree					426:431	The degree	422:431	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples	422:612	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	3	25	theme	pasting	521:527	arg1	substitution					436:447	substitution	436:447	substitution	436:447	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	3	25	theme	pasting	521:527	arg1	properties					529:538	pasting properties	521:538	pasting properties	521:538	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	9	26	theme	subsequent	1392:1401	arg1	succinylation					1403:1415	subsequent succinylation	1392:1415	subsequent succinylation	1392:1415	CONCLUSION Isolation and subsequent succinylation improved the wild yam starch quality and reduced its anti-nutritional factors.
32845257	2	27	theme	starch	387:392	arg1	%					385:385	88-100% starch	379:392	88-100% starch	379:392	Dioscorea villosa is an underutilized wild yam starch source and its starch was isolated and succinylated at 0, 3, 6, 9 and 12% (0-12% succinic anhydride: 88-100% starch) using standard procedures.
32845257	2	28	from	%	350:350	arg1	succinylated					317:328	succinylated	317:328	succinylated	317:328	Dioscorea villosa is an underutilized wild yam starch source and its starch was isolated and succinylated at 0, 3, 6, 9 and 12% (0-12% succinic anhydride: 88-100% starch) using standard procedures.
32845257	8	29	theme	temperature	1199:1209	arg1	values					1211:1216	peak time and pasting temperature values	1177:1216	values	1211:1216	The peak viscosity, trough, break down, final viscosity, set back, peak time and pasting temperature values ranged from 94.32-274.69 RVU, 83.50-197.50 RVU, 7.94-81.28 RVU, 113.69-367.39 RVU, 30.14-173.89 RVU, 5.30-6.94 min, and 84.78-94.70 °C respectively.
32845257	9	30	dep	CONCLUSION	1367:1376	arg1	Isolation					1378:1386	Isolation	1378:1386	Isolation	1378:1386	CONCLUSION Isolation and subsequent succinylation improved the wild yam starch quality and reduced its anti-nutritional factors.
32845257	9	30	dep	CONCLUSION	1367:1376	arg1	succinylation					1403:1415	subsequent succinylation	1392:1415	subsequent succinylation	1392:1415	CONCLUSION Isolation and subsequent succinylation improved the wild yam starch quality and reduced its anti-nutritional factors.
32845257	0	31	theme	yam	76:78	arg1	starch					99:104	wild yam (Discorea villosa) starch	71:104	wild yam (Discorea villosa) starch	71:104	Value addition and influence of succinylation levels on the quality of wild yam (Discorea villosa) starch.
32845257	9	32	theme	wild	1430:1433	arg1	quality					1446:1452	the wild yam starch quality	1426:1452	the wild yam starch quality	1426:1452	CONCLUSION Isolation and subsequent succinylation improved the wild yam starch quality and reduced its anti-nutritional factors.
32845257	0	33	theme	Discorea	81:88	arg1	starch					99:104	wild yam (Discorea villosa) starch	71:104	wild yam (Discorea villosa) starch	71:104	Value addition and influence of succinylation levels on the quality of wild yam (Discorea villosa) starch.
32845257	9	34	theme	starch	1439:1444	arg1	quality					1446:1452	the wild yam starch quality	1426:1452	the wild yam starch quality	1426:1452	CONCLUSION Isolation and subsequent succinylation improved the wild yam starch quality and reduced its anti-nutritional factors.
32845257	2	35	theme	%	357:357	arg1	anhydride					368:376	0-12% succinic anhydride	353:376	0-12% succinic anhydride	353:376	Dioscorea villosa is an underutilized wild yam starch source and its starch was isolated and succinylated at 0, 3, 6, 9 and 12% (0-12% succinic anhydride: 88-100% starch) using standard procedures.
32845257	2	35	theme	%	357:357	arg1	%					350:350	0, 3, 6, 9 and 12%	333:350	0, 3, 6, 9 and 12% (0-12% succinic anhydride: 88-100% starch) using standard procedures	333:419	Dioscorea villosa is an underutilized wild yam starch source and its starch was isolated and succinylated at 0, 3, 6, 9 and 12% (0-12% succinic anhydride: 88-100% starch) using standard procedures.
32845257	11	36	from	desirable	1727:1735	arg1	production					1744:1753	the production	1740:1753	the production of slowly digestible food products, such as snacks for diabetic patients	1740:1826	Its limited enzyme activities make it desirable in the production of slowly digestible food products, such as snacks for diabetic patients.
32845257	11	37	theme	food	1776:1779	arg1	snacks					1799:1804	snacks	1799:1804	snacks for diabetic patients	1799:1826	Its limited enzyme activities make it desirable in the production of slowly digestible food products, such as snacks for diabetic patients.
32845257	11	37	theme	food	1776:1779	arg1	products					1781:1788	slowly digestible food products	1758:1788	slowly digestible food products	1758:1788	Its limited enzyme activities make it desirable in the production of slowly digestible food products, such as snacks for diabetic patients.
32845257	0	38	theme	Value	0:4	arg1	addition					6:13	Value addition	0:13	Value addition	0:13	Value addition and influence of succinylation levels on the quality of wild yam (Discorea villosa) starch.
32845257	7	39	theme	g-1	1071:1073	arg1	g-1					1092:1094	0.08-0.13 mg g-1, 0.04-0.08 mg g-1, 0.21-0.31 mg g-1 and 0.18-0.26 mg g-1	1022:1094	0.08-0.13 mg g-1, 0.04-0.08 mg g-1, 0.21-0.31 mg g-1 and 0.18-0.26 mg g-1 respectively	1022:1107	The phytate, tannin, oxalate and saponin contents ranged from 0.08-0.13 mg g-1, 0.04-0.08 mg g-1, 0.21-0.31 mg g-1 and 0.18-0.26 mg g-1 respectively.
32845257	3	40	theme	α-amylase	560:568	arg1	substitution					436:447	substitution	436:447	substitution	436:447	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	3	40	theme	α-amylase	560:568	arg1	activities					588:597	α-amylase and α-glucosidase activities	560:597	α-amylase and α-glucosidase activities	560:597	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	7	41	theme	0.21-0.31	1058:1066	arg1	mg					1068:1069	mg	1068:1069	mg	1068:1069	The phytate, tannin, oxalate and saponin contents ranged from 0.08-0.13 mg g-1, 0.04-0.08 mg g-1, 0.21-0.31 mg g-1 and 0.18-0.26 mg g-1 respectively.
32845257	5	42	theme	succinyl	722:729	arg1	content					731:737	succinyl content	722:737	succinyl content	722:737	RESULTS The percentage succinyl content and degree of substitution increased from 0-10.45% and 0-0.19% respectively.
32845257	3	43	theme	α-glucosidase	574:586	arg1	substitution					436:447	substitution	436:447	substitution	436:447	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	3	43	theme	α-glucosidase	574:586	arg1	activities					588:597	α-amylase and α-glucosidase activities	560:597	α-amylase and α-glucosidase activities	560:597	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	1	44	theme	other	205:209	arg1	sources					215:221	other new sources	205:221	other new sources	205:221	BACKGROUND Recent increase in demand for starch in food applications has called for research into other new sources.
32845257	6	45	theme	α-amylase	842:850	arg1	activities					870:879	The amylose, amylopectin, α-amylase and α-glucosidase activities	816:879	The amylose, amylopectin, α-amylase and α-glucosidase activities	816:879	The amylose, amylopectin, α-amylase and α-glucosidase activities varied from 49.88-59.70%, 40.29-50.11%, 0.17-0.96 and 0.62-10.07 respectively.
32845257	10	46	contain	had	1516:1518	arg2	properties					1552:1561	functional and pasting properties	1529:1561	the best functional and pasting properties	1520:1561	Succinylation at 9% had the best functional and pasting properties, therefore making it suitable as a potential emulsifier, fat replacer to prevent obesity and stabilizer in food applications.
32845257	10	46	contain	had	1516:1518	arg1	Succinylation					1496:1508	Succinylation	1496:1508	Succinylation at 9%	1496:1514	Succinylation at 9% had the best functional and pasting properties, therefore making it suitable as a potential emulsifier, fat replacer to prevent obesity and stabilizer in food applications.
32845257	10	47	theme	potential	1598:1606	arg1	emulsifier					1608:1617	a potential emulsifier	1596:1617	a potential emulsifier	1596:1617	Succinylation at 9% had the best functional and pasting properties, therefore making it suitable as a potential emulsifier, fat replacer to prevent obesity and stabilizer in food applications.
32845257	10	47	theme	potential	1598:1606	arg1	replacer					1624:1631	fat replacer	1620:1631	fat replacer to prevent obesity and stabilizer in food applications	1620:1686	Succinylation at 9% had the best functional and pasting properties, therefore making it suitable as a potential emulsifier, fat replacer to prevent obesity and stabilizer in food applications.
32845257	6	48	theme	amylopectin	829:839	arg1	activities					870:879	The amylose, amylopectin, α-amylase and α-glucosidase activities	816:879	The amylose, amylopectin, α-amylase and α-glucosidase activities	816:879	The amylose, amylopectin, α-amylase and α-glucosidase activities varied from 49.88-59.70%, 40.29-50.11%, 0.17-0.96 and 0.62-10.07 respectively.
32845257	3	49	theme	samples	606:612	arg1	factors					512:518	anti-nutritional factors	495:518	anti-nutritional factors	495:518	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	3	49	theme	samples	606:612	arg1	spectra					548:554	atomic spectra	541:554	atomic spectra	541:554	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	3	49	theme	samples	606:612	arg1	properties					529:538	pasting properties	521:538	pasting properties	521:538	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	3	49	theme	samples	606:612	arg1	substitution					436:447	substitution	436:447	substitution	436:447	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	3	49	theme	samples	606:612	arg1	properties					483:492	functional properties	472:492	functional properties	472:492	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	3	49	theme	samples	606:612	arg1	activities					588:597	α-amylase and α-glucosidase activities	560:597	α-amylase and α-glucosidase activities	560:597	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	7	50	theme	mg	1032:1033	arg1	g-1					1035:1037	0.08-0.13 mg g-1	1022:1037	0.08-0.13 mg g-1	1022:1037	The phytate, tannin, oxalate and saponin contents ranged from 0.08-0.13 mg g-1, 0.04-0.08 mg g-1, 0.21-0.31 mg g-1 and 0.18-0.26 mg g-1 respectively.
32845257	7	50	theme	mg	1032:1033	arg1	mg					1050:1051	0.04-0.08 mg	1040:1051	0.04-0.08 mg	1040:1051	The phytate, tannin, oxalate and saponin contents ranged from 0.08-0.13 mg g-1, 0.04-0.08 mg g-1, 0.21-0.31 mg g-1 and 0.18-0.26 mg g-1 respectively.
32845257	2	51	theme	wild	262:265	arg1	source					278:283	an underutilized wild yam starch source	245:283	an underutilized wild yam starch source	245:283	Dioscorea villosa is an underutilized wild yam starch source and its starch was isolated and succinylated at 0, 3, 6, 9 and 12% (0-12% succinic anhydride: 88-100% starch) using standard procedures.
32845257	2	51	theme	wild	262:265	arg1	villosa					234:240	Dioscorea villosa	224:240	Dioscorea villosa	224:240	Dioscorea villosa is an underutilized wild yam starch source and its starch was isolated and succinylated at 0, 3, 6, 9 and 12% (0-12% succinic anhydride: 88-100% starch) using standard procedures.
32845257	6	52	theme	amylose	820:826	arg1	activities					870:879	The amylose, amylopectin, α-amylase and α-glucosidase activities	816:879	The amylose, amylopectin, α-amylase and α-glucosidase activities	816:879	The amylose, amylopectin, α-amylase and α-glucosidase activities varied from 49.88-59.70%, 40.29-50.11%, 0.17-0.96 and 0.62-10.07 respectively.
32845257	0	53	theme	levels	46:51	arg1	influence					19:27	influence	19:27	influence of succinylation levels on the quality of wild yam (Discorea villosa) starch	19:104	Value addition and influence of succinylation levels on the quality of wild yam (Discorea villosa) starch.
32845257	0	53	theme	levels	46:51	arg1	addition					6:13	Value addition	0:13	Value addition	0:13	Value addition and influence of succinylation levels on the quality of wild yam (Discorea villosa) starch.
32845257	3	54	theme	standard	636:643	arg1	methods					645:651	standard methods	636:651	standard methods	636:651	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	8	55	theme	break	1138:1142	arg1	down					1144:1147	break down	1138:1147	break down	1138:1147	The peak viscosity, trough, break down, final viscosity, set back, peak time and pasting temperature values ranged from 94.32-274.69 RVU, 83.50-197.50 RVU, 7.94-81.28 RVU, 113.69-367.39 RVU, 30.14-173.89 RVU, 5.30-6.94 min, and 84.78-94.70 °C respectively.
32845257	8	55	theme	break	1138:1142	arg1	viscosity					1119:1127	The peak viscosity	1110:1127	The peak viscosity	1110:1127	The peak viscosity, trough, break down, final viscosity, set back, peak time and pasting temperature values ranged from 94.32-274.69 RVU, 83.50-197.50 RVU, 7.94-81.28 RVU, 113.69-367.39 RVU, 30.14-173.89 RVU, 5.30-6.94 min, and 84.78-94.70 °C respectively.
32845257	0	56	theme	starch	99:104	arg1	quality					60:66	the quality	56:66	the quality of wild yam (Discorea villosa) starch	56:104	Value addition and influence of succinylation levels on the quality of wild yam (Discorea villosa) starch.
32845257	2	57	theme	starch	271:276	arg1	source					278:283	an underutilized wild yam starch source	245:283	an underutilized wild yam starch source	245:283	Dioscorea villosa is an underutilized wild yam starch source and its starch was isolated and succinylated at 0, 3, 6, 9 and 12% (0-12% succinic anhydride: 88-100% starch) using standard procedures.
32845257	2	57	theme	starch	271:276	arg1	villosa					234:240	Dioscorea villosa	224:240	Dioscorea villosa	224:240	Dioscorea villosa is an underutilized wild yam starch source and its starch was isolated and succinylated at 0, 3, 6, 9 and 12% (0-12% succinic anhydride: 88-100% starch) using standard procedures.
32845257	5	58	theme	substitution	753:764	arg1	percentage					711:720	The percentage succinyl content and degree	707:748	percentage	711:720	RESULTS The percentage succinyl content and degree of substitution increased from 0-10.45% and 0-0.19% respectively.
32845257	5	58	theme	substitution	753:764	arg1	degree					743:748	degree	743:748	degree	743:748	RESULTS The percentage succinyl content and degree of substitution increased from 0-10.45% and 0-0.19% respectively.
32845257	3	59	theme	anti-nutritional	495:510	arg1	substitution					436:447	substitution	436:447	substitution	436:447	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	3	59	theme	anti-nutritional	495:510	arg1	factors					512:518	anti-nutritional factors	495:518	anti-nutritional factors	495:518	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	6	60	theme	α-glucosidase	856:868	arg1	activities					870:879	The amylose, amylopectin, α-amylase and α-glucosidase activities	816:879	The amylose, amylopectin, α-amylase and α-glucosidase activities	816:879	The amylose, amylopectin, α-amylase and α-glucosidase activities varied from 49.88-59.70%, 40.29-50.11%, 0.17-0.96 and 0.62-10.07 respectively.
32845257	11	61	theme	enzyme	1701:1706	arg1	activities					1708:1717	Its limited enzyme activities	1689:1717	Its limited enzyme activities	1689:1717	Its limited enzyme activities make it desirable in the production of slowly digestible food products, such as snacks for diabetic patients.
32845257	3	62	theme	chemical	450:457	arg1	substitution					436:447	substitution	436:447	substitution	436:447	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	3	62	theme	chemical	450:457	arg1	composition					459:469	chemical composition	450:469	chemical composition	450:469	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	1	63	theme	Recent	118:123	arg1	increase					125:132	BACKGROUND Recent increase	107:132	BACKGROUND Recent increase in demand for starch in food applications	107:174	BACKGROUND Recent increase in demand for starch in food applications has called for research into other new sources.
32845257	0	64	from	influence	19:27	arg1	quality					60:66	the quality	56:66	the quality of wild yam (Discorea villosa) starch	56:104	Value addition and influence of succinylation levels on the quality of wild yam (Discorea villosa) starch.
32845257	0	65	theme	wild	71:74	arg1	starch					99:104	wild yam (Discorea villosa) starch	71:104	wild yam (Discorea villosa) starch	71:104	Value addition and influence of succinylation levels on the quality of wild yam (Discorea villosa) starch.
32845257	2	66	dep	anhydride	368:376	arg1	%					385:385	88-100% starch	379:392	88-100% starch	379:392	Dioscorea villosa is an underutilized wild yam starch source and its starch was isolated and succinylated at 0, 3, 6, 9 and 12% (0-12% succinic anhydride: 88-100% starch) using standard procedures.
32845257	10	67	from	obesity	1644:1650	arg1	applications					1675:1686	food applications	1670:1686	food applications	1670:1686	Succinylation at 9% had the best functional and pasting properties, therefore making it suitable as a potential emulsifier, fat replacer to prevent obesity and stabilizer in food applications.
32845257	0	68	from	addition	6:13	arg1	quality					60:66	the quality	56:66	the quality of wild yam (Discorea villosa) starch	56:104	Value addition and influence of succinylation levels on the quality of wild yam (Discorea villosa) starch.
32845257	11	69	from	production	1744:1753	arg1	desirable					1727:1735	desirable	1727:1735	desirable	1727:1735	Its limited enzyme activities make it desirable in the production of slowly digestible food products, such as snacks for diabetic patients.
32845257	8	70	theme	pasting	1191:1197	arg1	values					1211:1216	peak time and pasting temperature values	1177:1216	values	1211:1216	The peak viscosity, trough, break down, final viscosity, set back, peak time and pasting temperature values ranged from 94.32-274.69 RVU, 83.50-197.50 RVU, 7.94-81.28 RVU, 113.69-367.39 RVU, 30.14-173.89 RVU, 5.30-6.94 min, and 84.78-94.70 °C respectively.
32845257	10	71	from	stabilizer	1656:1665	arg1	applications					1675:1686	food applications	1670:1686	food applications	1670:1686	Succinylation at 9% had the best functional and pasting properties, therefore making it suitable as a potential emulsifier, fat replacer to prevent obesity and stabilizer in food applications.
32845257	0	72	theme	villosa	90:96	arg1	starch					99:104	wild yam (Discorea villosa) starch	71:104	wild yam (Discorea villosa) starch	71:104	Value addition and influence of succinylation levels on the quality of wild yam (Discorea villosa) starch.
32845257	1	73	from	increase	125:132	arg1	applications					163:174	food applications	158:174	food applications	158:174	BACKGROUND Recent increase in demand for starch in food applications has called for research into other new sources.
32845257	1	73	from	increase	125:132	arg1	demand					137:142	demand	137:142	demand for starch	137:153	BACKGROUND Recent increase in demand for starch in food applications has called for research into other new sources.
32845257	9	74	theme	yam	1435:1437	arg1	quality					1446:1452	the wild yam starch quality	1426:1452	the wild yam starch quality	1426:1452	CONCLUSION Isolation and subsequent succinylation improved the wild yam starch quality and reduced its anti-nutritional factors.
32845257	2	75	theme	standard	401:408	arg1	procedures					410:419	standard procedures	401:419	standard procedures	401:419	Dioscorea villosa is an underutilized wild yam starch source and its starch was isolated and succinylated at 0, 3, 6, 9 and 12% (0-12% succinic anhydride: 88-100% starch) using standard procedures.
32845257	7	76	theme	mg	1089:1090	arg1	g-1					1092:1094	0.08-0.13 mg g-1, 0.04-0.08 mg g-1, 0.21-0.31 mg g-1 and 0.18-0.26 mg g-1	1022:1094	0.08-0.13 mg g-1, 0.04-0.08 mg g-1, 0.21-0.31 mg g-1 and 0.18-0.26 mg g-1 respectively	1022:1107	The phytate, tannin, oxalate and saponin contents ranged from 0.08-0.13 mg g-1, 0.04-0.08 mg g-1, 0.21-0.31 mg g-1 and 0.18-0.26 mg g-1 respectively.
32845257	11	77	theme	products	1781:1788	arg1	production					1744:1753	the production	1740:1753	the production of slowly digestible food products, such as snacks for diabetic patients	1740:1826	Its limited enzyme activities make it desirable in the production of slowly digestible food products, such as snacks for diabetic patients.
32845257	7	78	theme	mg	1068:1069	arg1	g-1					1071:1073	0.21-0.31 mg g-1	1058:1073	0.21-0.31 mg g-1	1058:1073	The phytate, tannin, oxalate and saponin contents ranged from 0.08-0.13 mg g-1, 0.04-0.08 mg g-1, 0.21-0.31 mg g-1 and 0.18-0.26 mg g-1 respectively.
32845257	11	79	theme	diabetic	1810:1817	arg1	patients					1819:1826	diabetic patients	1810:1826	diabetic patients	1810:1826	Its limited enzyme activities make it desirable in the production of slowly digestible food products, such as snacks for diabetic patients.
32845257	0	80	theme	succinylation	32:44	arg1	levels					46:51	succinylation levels	32:51	succinylation levels	32:51	Value addition and influence of succinylation levels on the quality of wild yam (Discorea villosa) starch.
32845257	9	81	theme	anti-nutritional	1470:1485	arg1	factors					1487:1493	its anti-nutritional factors	1466:1493	its anti-nutritional factors	1466:1493	CONCLUSION Isolation and subsequent succinylation improved the wild yam starch quality and reduced its anti-nutritional factors.
32845257	3	82	theme	atomic	541:546	arg1	substitution					436:447	substitution	436:447	substitution	436:447	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	3	82	theme	atomic	541:546	arg1	spectra					548:554	atomic spectra	541:554	atomic spectra	541:554	The degree of substitution, chemical composition, functional properties, anti-nutritional factors, pasting properties, atomic spectra and α-amylase and α-glucosidase activities of the samples were determined using standard methods.
32845257	10	83	theme	pasting	1544:1550	arg1	properties					1552:1561	functional and pasting properties	1529:1561	the best functional and pasting properties	1520:1561	Succinylation at 9% had the best functional and pasting properties, therefore making it suitable as a potential emulsifier, fat replacer to prevent obesity and stabilizer in food applications.
32845257	2	84	theme	succinic	359:366	arg1	anhydride					368:376	0-12% succinic anhydride	353:376	0-12% succinic anhydride	353:376	Dioscorea villosa is an underutilized wild yam starch source and its starch was isolated and succinylated at 0, 3, 6, 9 and 12% (0-12% succinic anhydride: 88-100% starch) using standard procedures.
32845257	2	84	theme	succinic	359:366	arg1	%					350:350	0, 3, 6, 9 and 12%	333:350	0, 3, 6, 9 and 12% (0-12% succinic anhydride: 88-100% starch) using standard procedures	333:419	Dioscorea villosa is an underutilized wild yam starch source and its starch was isolated and succinylated at 0, 3, 6, 9 and 12% (0-12% succinic anhydride: 88-100% starch) using standard procedures.
32845257	10	85	theme	functional	1529:1538	arg1	properties					1552:1561	functional and pasting properties	1529:1561	the best functional and pasting properties	1520:1561	Succinylation at 9% had the best functional and pasting properties, therefore making it suitable as a potential emulsifier, fat replacer to prevent obesity and stabilizer in food applications.
32845257	11	86	theme	digestible	1765:1774	arg1	snacks					1799:1804	snacks	1799:1804	snacks for diabetic patients	1799:1826	Its limited enzyme activities make it desirable in the production of slowly digestible food products, such as snacks for diabetic patients.
32845257	11	86	theme	digestible	1765:1774	arg1	products					1781:1788	slowly digestible food products	1758:1788	slowly digestible food products	1758:1788	Its limited enzyme activities make it desirable in the production of slowly digestible food products, such as snacks for diabetic patients.
32845257	1	87	theme	new	211:213	arg1	sources					215:221	other new sources	205:221	other new sources	205:221	BACKGROUND Recent increase in demand for starch in food applications has called for research into other new sources.
34889593	0	0	theme	Good	99:102	arg1	Properties					158:167	Good Electromagnetic Shielding Biocompatible Antibacterial Properties	99:167	Good Electromagnetic Shielding Biocompatible Antibacterial Properties	99:167	Highly Sensitive Multifunctional Electronic Skin Based on Nanocellulose/MXene Composite Films with Good Electromagnetic Shielding Biocompatible Antibacterial Properties.
34889593	1	1	theme	extensive	198:206	arg1	interest					217:224	aroused extensive research interest	190:224	aroused extensive research interest	190:224	Electronic skin has aroused extensive research interest due to high similarity with human skin.
34889593	1	2	theme	research	208:215	arg1	interest					217:224	aroused extensive research interest	190:224	aroused extensive research interest	190:224	Electronic skin has aroused extensive research interest due to high similarity with human skin.
34889593	0	3	theme	Shielding	120:128	arg1	Properties					158:167	Good Electromagnetic Shielding Biocompatible Antibacterial Properties	99:167	Good Electromagnetic Shielding Biocompatible Antibacterial Properties	99:167	Highly Sensitive Multifunctional Electronic Skin Based on Nanocellulose/MXene Composite Films with Good Electromagnetic Shielding Biocompatible Antibacterial Properties.
34889593	6	4	theme	piezoresistive	986:999	arg1	sensor					1001:1006	the piezoresistive sensor	982:1006	the piezoresistive sensor assembled from the composite film	982:1040	In addition, the piezoresistive sensor assembled from the composite film exhibited a high sensitivity (11.6 kPa-1), fast response and recovery time (≤10 ms), ultralow monitoring limit (0.2 Pa), and long-term stability (>10 000 cycles).
34889593	3	5	theme	conductive	563:572	arg1	dispersion					582:591	highly conductive Ti3C2TX dispersion	556:591	highly conductive Ti3C2TX dispersion	556:591	Here, we use 2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)-oxidized cellulose nanofibril (TOCN) dispersion and highly conductive Ti3C2TX dispersion to prepare TOCN/Ti3C2TX composite film through vacuum-assisted filtration.
34889593	2	6	theme	skin	341:344	arg1	functions					346:354	skin functions	341:354	skin functions	341:354	Realizing a multifunctional electronic skin that is highly consistent with skin functions and endowed with more other functions is now a more urgent need and important challenge.
34889593	4	7	theme	obtained	672:679	arg1	film					691:694	The obtained composite film	668:694	The obtained composite film imitating the nacre-like lamellar structure of natural shells	668:756	The obtained composite film imitating the nacre-like lamellar structure of natural shells has good mechanical properties (124.6 MPa of tensile strength).
34889593	0	8	theme	Electromagnetic	104:118	arg1	Properties					158:167	Good Electromagnetic Shielding Biocompatible Antibacterial Properties	99:167	Good Electromagnetic Shielding Biocompatible Antibacterial Properties	99:167	Highly Sensitive Multifunctional Electronic Skin Based on Nanocellulose/MXene Composite Films with Good Electromagnetic Shielding Biocompatible Antibacterial Properties.
34889593	0	9	with	Films	88:92	arg1	Properties					158:167	Good Electromagnetic Shielding Biocompatible Antibacterial Properties	99:167	Good Electromagnetic Shielding Biocompatible Antibacterial Properties	99:167	Highly Sensitive Multifunctional Electronic Skin Based on Nanocellulose/MXene Composite Films with Good Electromagnetic Shielding Biocompatible Antibacterial Properties.
34889593	3	10	theme	TOCN/Ti3C2TX	604:615	arg1	film					627:630	TOCN/Ti3C2TX composite film	604:630	TOCN/Ti3C2TX composite film	604:630	Here, we use 2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)-oxidized cellulose nanofibril (TOCN) dispersion and highly conductive Ti3C2TX dispersion to prepare TOCN/Ti3C2TX composite film through vacuum-assisted filtration.
34889593	4	11	theme	tensile	803:809	arg1	strength					811:818	tensile strength	803:818	tensile strength	803:818	The obtained composite film imitating the nacre-like lamellar structure of natural shells has good mechanical properties (124.6 MPa of tensile strength).
34889593	0	12	theme	Antibacterial	144:156	arg1	Properties					158:167	Good Electromagnetic Shielding Biocompatible Antibacterial Properties	99:167	Good Electromagnetic Shielding Biocompatible Antibacterial Properties	99:167	Highly Sensitive Multifunctional Electronic Skin Based on Nanocellulose/MXene Composite Films with Good Electromagnetic Shielding Biocompatible Antibacterial Properties.
34889593	4	13	theme	nacre-like	710:719	arg1	structure					730:738	the nacre-like lamellar structure	706:738	the nacre-like lamellar structure of natural shells	706:756	The obtained composite film imitating the nacre-like lamellar structure of natural shells has good mechanical properties (124.6 MPa of tensile strength).
34889593	4	14	dep	properties	778:787	arg1	MPa					796:798	124.6 MPa	790:798	124.6 MPa of tensile strength	790:818	The obtained composite film imitating the nacre-like lamellar structure of natural shells has good mechanical properties (124.6 MPa of tensile strength).
34889593	4	15	theme	shells	751:756	arg1	structure					730:738	the nacre-like lamellar structure	706:738	the nacre-like lamellar structure of natural shells	706:756	The obtained composite film imitating the nacre-like lamellar structure of natural shells has good mechanical properties (124.6 MPa of tensile strength).
34889593	0	16	theme	Biocompatible	130:142	arg1	Properties					158:167	Good Electromagnetic Shielding Biocompatible Antibacterial Properties	99:167	Good Electromagnetic Shielding Biocompatible Antibacterial Properties	99:167	Highly Sensitive Multifunctional Electronic Skin Based on Nanocellulose/MXene Composite Films with Good Electromagnetic Shielding Biocompatible Antibacterial Properties.
34889593	4	17	theme	composite	681:689	arg1	film					691:694	The obtained composite film	668:694	The obtained composite film imitating the nacre-like lamellar structure of natural shells	668:756	The obtained composite film imitating the nacre-like lamellar structure of natural shells has good mechanical properties (124.6 MPa of tensile strength).
34889593	2	18	theme	important	424:432	arg1	challenge					434:442	important challenge	424:442	important challenge	424:442	Realizing a multifunctional electronic skin that is highly consistent with skin functions and endowed with more other functions is now a more urgent need and important challenge.
34889593	0	19	theme	Sensitive	7:15	arg1	Skin					44:47	Highly Sensitive Multifunctional Electronic Skin	0:47	Highly Sensitive Multifunctional Electronic Skin	0:47	Highly Sensitive Multifunctional Electronic Skin Based on Nanocellulose/MXene Composite Films with Good Electromagnetic Shielding Biocompatible Antibacterial Properties.
34889593	1	20	contain	has	186:188	arg2	interest					217:224	aroused extensive research interest	190:224	aroused extensive research interest	190:224	Electronic skin has aroused extensive research interest due to high similarity with human skin.
34889593	1	20	contain	has	186:188	arg1	skin					181:184	Electronic skin	170:184	Electronic skin	170:184	Electronic skin has aroused extensive research interest due to high similarity with human skin.
34889593	6	21	theme	monitoring	1136:1145	arg1	limit					1147:1151	ultralow monitoring limit	1127:1151	ultralow monitoring limit (0.2 Pa)	1127:1160	In addition, the piezoresistive sensor assembled from the composite film exhibited a high sensitivity (11.6 kPa-1), fast response and recovery time (≤10 ms), ultralow monitoring limit (0.2 Pa), and long-term stability (>10 000 cycles).
34889593	6	21	theme	monitoring	1136:1145	arg1	Pa					1158:1159	0.2 Pa	1154:1159	0.2 Pa	1154:1159	In addition, the piezoresistive sensor assembled from the composite film exhibited a high sensitivity (11.6 kPa-1), fast response and recovery time (≤10 ms), ultralow monitoring limit (0.2 Pa), and long-term stability (>10 000 cycles).
34889593	4	22	theme	natural	743:749	arg1	shells					751:756	natural shells	743:756	natural shells	743:756	The obtained composite film imitating the nacre-like lamellar structure of natural shells has good mechanical properties (124.6 MPa of tensile strength).
34889593	4	23	theme	good	762:765	arg1	properties					778:787	good mechanical properties	762:787	good mechanical properties (124.6 MPa of tensile strength)	762:819	The obtained composite film imitating the nacre-like lamellar structure of natural shells has good mechanical properties (124.6 MPa of tensile strength).
34889593	6	24	theme	recovery	1103:1110	arg1	time					1112:1115	recovery time	1103:1115	recovery time	1103:1115	In addition, the piezoresistive sensor assembled from the composite film exhibited a high sensitivity (11.6 kPa-1), fast response and recovery time (≤10 ms), ultralow monitoring limit (0.2 Pa), and long-term stability (>10 000 cycles).
34889593	3	25	theme	vacuum-assisted	640:654	arg1	filtration					656:665	vacuum-assisted filtration	640:665	vacuum-assisted filtration	640:665	Here, we use 2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)-oxidized cellulose nanofibril (TOCN) dispersion and highly conductive Ti3C2TX dispersion to prepare TOCN/Ti3C2TX composite film through vacuum-assisted filtration.
34889593	1	26	theme	high	233:236	arg1	similarity					238:247	high similarity	233:247	high similarity with human skin	233:263	Electronic skin has aroused extensive research interest due to high similarity with human skin.
34889593	0	27	theme	Electronic	33:42	arg1	Skin					44:47	Highly Sensitive Multifunctional Electronic Skin	0:47	Highly Sensitive Multifunctional Electronic Skin	0:47	Highly Sensitive Multifunctional Electronic Skin Based on Nanocellulose/MXene Composite Films with Good Electromagnetic Shielding Biocompatible Antibacterial Properties.
34889593	6	28	theme	ultralow	1127:1134	arg1	limit					1147:1151	ultralow monitoring limit	1127:1151	ultralow monitoring limit (0.2 Pa)	1127:1160	In addition, the piezoresistive sensor assembled from the composite film exhibited a high sensitivity (11.6 kPa-1), fast response and recovery time (≤10 ms), ultralow monitoring limit (0.2 Pa), and long-term stability (>10 000 cycles).
34889593	6	28	theme	ultralow	1127:1134	arg1	Pa					1158:1159	0.2 Pa	1154:1159	0.2 Pa	1154:1159	In addition, the piezoresistive sensor assembled from the composite film exhibited a high sensitivity (11.6 kPa-1), fast response and recovery time (≤10 ms), ultralow monitoring limit (0.2 Pa), and long-term stability (>10 000 cycles).
34889593	3	29	theme	composite	617:625	arg1	film					627:630	TOCN/Ti3C2TX composite film	604:630	TOCN/Ti3C2TX composite film	604:630	Here, we use 2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)-oxidized cellulose nanofibril (TOCN) dispersion and highly conductive Ti3C2TX dispersion to prepare TOCN/Ti3C2TX composite film through vacuum-assisted filtration.
34889593	3	30	theme	cellulose	513:521	arg1	dispersion					541:550	2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)-oxidized cellulose nanofibril (TOCN) dispersion	458:550	2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)-oxidized cellulose nanofibril (TOCN) dispersion	458:550	Here, we use 2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)-oxidized cellulose nanofibril (TOCN) dispersion and highly conductive Ti3C2TX dispersion to prepare TOCN/Ti3C2TX composite film through vacuum-assisted filtration.
34889593	0	31	theme	Multifunctional	17:31	arg1	Skin					44:47	Highly Sensitive Multifunctional Electronic Skin	0:47	Highly Sensitive Multifunctional Electronic Skin	0:47	Highly Sensitive Multifunctional Electronic Skin Based on Nanocellulose/MXene Composite Films with Good Electromagnetic Shielding Biocompatible Antibacterial Properties.
34889593	6	32	theme	high	1054:1057	arg1	sensitivity					1059:1069	a high sensitivity	1052:1069	a high sensitivity (11.6 kPa-1)	1052:1082	In addition, the piezoresistive sensor assembled from the composite film exhibited a high sensitivity (11.6 kPa-1), fast response and recovery time (≤10 ms), ultralow monitoring limit (0.2 Pa), and long-term stability (>10 000 cycles).
34889593	6	32	theme	high	1054:1057	arg1	kPa-1					1077:1081	11.6 kPa-1	1072:1081	11.6 kPa-1	1072:1081	In addition, the piezoresistive sensor assembled from the composite film exhibited a high sensitivity (11.6 kPa-1), fast response and recovery time (≤10 ms), ultralow monitoring limit (0.2 Pa), and long-term stability (>10 000 cycles).
34889593	3	33	theme	nanofibril	523:532	arg1	dispersion					541:550	2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)-oxidized cellulose nanofibril (TOCN) dispersion	458:550	2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)-oxidized cellulose nanofibril (TOCN) dispersion	458:550	Here, we use 2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)-oxidized cellulose nanofibril (TOCN) dispersion and highly conductive Ti3C2TX dispersion to prepare TOCN/Ti3C2TX composite film through vacuum-assisted filtration.
34889593	1	34	with	similarity	238:247	arg1	skin					260:263	human skin	254:263	human skin	254:263	Electronic skin has aroused extensive research interest due to high similarity with human skin.
34889593	2	35	theme	multifunctional	278:292	arg1	skin					305:308	a multifunctional electronic skin	276:308	a multifunctional electronic skin that is highly consistent with skin functions and endowed with more other functions	276:392	Realizing a multifunctional electronic skin that is highly consistent with skin functions and endowed with more other functions is now a more urgent need and important challenge.
34889593	2	35	theme	multifunctional	278:292	arg1	consistent					325:334	consistent	325:334	consistent	325:334	Realizing a multifunctional electronic skin that is highly consistent with skin functions and endowed with more other functions is now a more urgent need and important challenge.
34889593	1	36	theme	human	254:258	arg1	skin					260:263	human skin	254:263	human skin	254:263	Electronic skin has aroused extensive research interest due to high similarity with human skin.
34889593	5	37	theme	excellent	864:872	arg1	performance					900:910	excellent electromagnetic shielding performance	864:910	excellent electromagnetic shielding performance (36 dB)	864:918	Meanwhile, the composite film also showed excellent electromagnetic shielding performance (36 dB), biocompatibility, and antibacterial properties.
34889593	5	37	theme	excellent	864:872	arg1	dB					916:917	36 dB	913:917	36 dB	913:917	Meanwhile, the composite film also showed excellent electromagnetic shielding performance (36 dB), biocompatibility, and antibacterial properties.
34889593	5	38	theme	antibacterial	943:955	arg1	properties					957:966	antibacterial properties	943:966	antibacterial properties	943:966	Meanwhile, the composite film also showed excellent electromagnetic shielding performance (36 dB), biocompatibility, and antibacterial properties.
34889593	3	39	theme	-oxidized	503:511	arg1	dispersion					541:550	2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)-oxidized cellulose nanofibril (TOCN) dispersion	458:550	2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)-oxidized cellulose nanofibril (TOCN) dispersion	458:550	Here, we use 2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)-oxidized cellulose nanofibril (TOCN) dispersion and highly conductive Ti3C2TX dispersion to prepare TOCN/Ti3C2TX composite film through vacuum-assisted filtration.
34889593	0	40	theme	Nanocellulose/MXene	58:76	arg1	Films					88:92	Nanocellulose/MXene Composite Films	58:92	Nanocellulose/MXene Composite Films with Good Electromagnetic Shielding Biocompatible Antibacterial Properties	58:167	Highly Sensitive Multifunctional Electronic Skin Based on Nanocellulose/MXene Composite Films with Good Electromagnetic Shielding Biocompatible Antibacterial Properties.
34889593	7	41	theme	human	1226:1230	arg1	so					1283:1284	so	1283:1284	so	1283:1284	It also could detect human daily activities such as finger bent, chewing, and so on.
34889593	7	41	theme	human	1226:1230	arg1	bent					1264:1267	finger bent	1257:1267	finger bent	1257:1267	It also could detect human daily activities such as finger bent, chewing, and so on.
34889593	7	41	theme	human	1226:1230	arg1	activities					1238:1247	human daily activities	1226:1247	human daily activities such as finger bent, chewing, and so on	1226:1287	It also could detect human daily activities such as finger bent, chewing, and so on.
34889593	5	42	theme	composite	837:845	arg1	Meanwhile					822:830	Meanwhile	822:830	Meanwhile	822:830	Meanwhile, the composite film also showed excellent electromagnetic shielding performance (36 dB), biocompatibility, and antibacterial properties.
34889593	5	42	theme	composite	837:845	arg1	film					847:850	the composite film	833:850	the composite film	833:850	Meanwhile, the composite film also showed excellent electromagnetic shielding performance (36 dB), biocompatibility, and antibacterial properties.
34889593	6	43	theme	long-term	1167:1175	arg1	stability					1177:1185	long-term stability	1167:1185	long-term stability (>10 000 cycles)	1167:1202	In addition, the piezoresistive sensor assembled from the composite film exhibited a high sensitivity (11.6 kPa-1), fast response and recovery time (≤10 ms), ultralow monitoring limit (0.2 Pa), and long-term stability (>10 000 cycles).
34889593	6	43	theme	long-term	1167:1175	arg1	cycles					1196:1201	>10 000 cycles	1188:1201	>10 000 cycles	1188:1201	In addition, the piezoresistive sensor assembled from the composite film exhibited a high sensitivity (11.6 kPa-1), fast response and recovery time (≤10 ms), ultralow monitoring limit (0.2 Pa), and long-term stability (>10 000 cycles).
34889593	5	44	theme	shielding	890:898	arg1	performance					900:910	excellent electromagnetic shielding performance	864:910	excellent electromagnetic shielding performance (36 dB)	864:918	Meanwhile, the composite film also showed excellent electromagnetic shielding performance (36 dB), biocompatibility, and antibacterial properties.
34889593	5	44	theme	shielding	890:898	arg1	dB					916:917	36 dB	913:917	36 dB	913:917	Meanwhile, the composite film also showed excellent electromagnetic shielding performance (36 dB), biocompatibility, and antibacterial properties.
34889593	6	45	theme	composite	1027:1035	arg1	film					1037:1040	the composite film	1023:1040	the composite film	1023:1040	In addition, the piezoresistive sensor assembled from the composite film exhibited a high sensitivity (11.6 kPa-1), fast response and recovery time (≤10 ms), ultralow monitoring limit (0.2 Pa), and long-term stability (>10 000 cycles).
34889593	4	46	contain	has	758:760	arg1	film					691:694	The obtained composite film	668:694	The obtained composite film imitating the nacre-like lamellar structure of natural shells	668:756	The obtained composite film imitating the nacre-like lamellar structure of natural shells has good mechanical properties (124.6 MPa of tensile strength).
34889593	4	46	contain	has	758:760	arg2	properties					778:787	good mechanical properties	762:787	good mechanical properties (124.6 MPa of tensile strength)	762:819	The obtained composite film imitating the nacre-like lamellar structure of natural shells has good mechanical properties (124.6 MPa of tensile strength).
34889593	7	47	theme	daily	1232:1236	arg1	so					1283:1284	so	1283:1284	so	1283:1284	It also could detect human daily activities such as finger bent, chewing, and so on.
34889593	7	47	theme	daily	1232:1236	arg1	bent					1264:1267	finger bent	1257:1267	finger bent	1257:1267	It also could detect human daily activities such as finger bent, chewing, and so on.
34889593	7	47	theme	daily	1232:1236	arg1	activities					1238:1247	human daily activities	1226:1247	human daily activities such as finger bent, chewing, and so on	1226:1287	It also could detect human daily activities such as finger bent, chewing, and so on.
34889593	4	48	theme	strength	811:818	arg1	MPa					796:798	124.6 MPa	790:798	124.6 MPa of tensile strength	790:818	The obtained composite film imitating the nacre-like lamellar structure of natural shells has good mechanical properties (124.6 MPa of tensile strength).
34889593	5	49	theme	electromagnetic	874:888	arg1	performance					900:910	excellent electromagnetic shielding performance	864:910	excellent electromagnetic shielding performance (36 dB)	864:918	Meanwhile, the composite film also showed excellent electromagnetic shielding performance (36 dB), biocompatibility, and antibacterial properties.
34889593	5	49	theme	electromagnetic	874:888	arg1	dB					916:917	36 dB	913:917	36 dB	913:917	Meanwhile, the composite film also showed excellent electromagnetic shielding performance (36 dB), biocompatibility, and antibacterial properties.
34889593	4	50	theme	mechanical	767:776	arg1	properties					778:787	good mechanical properties	762:787	good mechanical properties (124.6 MPa of tensile strength)	762:819	The obtained composite film imitating the nacre-like lamellar structure of natural shells has good mechanical properties (124.6 MPa of tensile strength).
34889593	1	51	theme	Electronic	170:179	arg1	skin					181:184	Electronic skin	170:184	Electronic skin	170:184	Electronic skin has aroused extensive research interest due to high similarity with human skin.
34889593	2	52	with	consistent	325:334	arg1	functions					384:392	more other functions	373:392	more other functions	373:392	Realizing a multifunctional electronic skin that is highly consistent with skin functions and endowed with more other functions is now a more urgent need and important challenge.
34889593	2	52	with	consistent	325:334	arg1	functions					346:354	skin functions	341:354	skin functions	341:354	Realizing a multifunctional electronic skin that is highly consistent with skin functions and endowed with more other functions is now a more urgent need and important challenge.
34889593	3	53	theme	TOCN	535:538	arg1	dispersion					541:550	2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)-oxidized cellulose nanofibril (TOCN) dispersion	458:550	2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)-oxidized cellulose nanofibril (TOCN) dispersion	458:550	Here, we use 2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)-oxidized cellulose nanofibril (TOCN) dispersion and highly conductive Ti3C2TX dispersion to prepare TOCN/Ti3C2TX composite film through vacuum-assisted filtration.
34889593	2	54	theme	other	378:382	arg1	functions					384:392	more other functions	373:392	more other functions	373:392	Realizing a multifunctional electronic skin that is highly consistent with skin functions and endowed with more other functions is now a more urgent need and important challenge.
34889593	7	55	theme	finger	1257:1262	arg1	chewing					1270:1276	chewing	1270:1276	chewing	1270:1276	It also could detect human daily activities such as finger bent, chewing, and so on.
34889593	7	55	theme	finger	1257:1262	arg1	bent					1264:1267	finger bent	1257:1267	finger bent	1257:1267	It also could detect human daily activities such as finger bent, chewing, and so on.
34889593	4	56	theme	lamellar	721:728	arg1	structure					730:738	the nacre-like lamellar structure	706:738	the nacre-like lamellar structure of natural shells	706:756	The obtained composite film imitating the nacre-like lamellar structure of natural shells has good mechanical properties (124.6 MPa of tensile strength).
34889593	2	57	theme	urgent	408:413	arg1	need					415:418	a more urgent need	401:418	a more urgent need	401:418	Realizing a multifunctional electronic skin that is highly consistent with skin functions and endowed with more other functions is now a more urgent need and important challenge.
34889593	3	58	theme	Ti3C2TX	574:580	arg1	dispersion					582:591	highly conductive Ti3C2TX dispersion	556:591	highly conductive Ti3C2TX dispersion	556:591	Here, we use 2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)-oxidized cellulose nanofibril (TOCN) dispersion and highly conductive Ti3C2TX dispersion to prepare TOCN/Ti3C2TX composite film through vacuum-assisted filtration.
34889593	0	59	theme	Composite	78:86	arg1	Films					88:92	Nanocellulose/MXene Composite Films	58:92	Nanocellulose/MXene Composite Films with Good Electromagnetic Shielding Biocompatible Antibacterial Properties	58:167	Highly Sensitive Multifunctional Electronic Skin Based on Nanocellulose/MXene Composite Films with Good Electromagnetic Shielding Biocompatible Antibacterial Properties.
34889593	2	60	theme	electronic	294:303	arg1	skin					305:308	a multifunctional electronic skin	276:308	a multifunctional electronic skin that is highly consistent with skin functions and endowed with more other functions	276:392	Realizing a multifunctional electronic skin that is highly consistent with skin functions and endowed with more other functions is now a more urgent need and important challenge.
34889593	2	60	theme	electronic	294:303	arg1	consistent					325:334	consistent	325:334	consistent	325:334	Realizing a multifunctional electronic skin that is highly consistent with skin functions and endowed with more other functions is now a more urgent need and important challenge.
34889593	1	61	theme	aroused	190:196	arg1	interest					217:224	aroused extensive research interest	190:224	aroused extensive research interest	190:224	Electronic skin has aroused extensive research interest due to high similarity with human skin.
34889593	6	62	theme	fast	1085:1088	arg1	response					1090:1097	fast response	1085:1097	fast response	1085:1097	In addition, the piezoresistive sensor assembled from the composite film exhibited a high sensitivity (11.6 kPa-1), fast response and recovery time (≤10 ms), ultralow monitoring limit (0.2 Pa), and long-term stability (>10 000 cycles).
34889593	6	62	theme	fast	1085:1088	arg1	ms					1122:1123	≤10 ms	1118:1123	≤10 ms	1118:1123	In addition, the piezoresistive sensor assembled from the composite film exhibited a high sensitivity (11.6 kPa-1), fast response and recovery time (≤10 ms), ultralow monitoring limit (0.2 Pa), and long-term stability (>10 000 cycles).
34919998	6	0	dep	range	939:943	arg1	2.16-3.67 μm					951:962	2.16-3.67 μm	951:962	2.16-3.67 μm	951:962	The particle size remained in a relatively narrow range (D50, 2.16-3.67 μm), and therefore was considered independent of the factors investigated.
34919998	6	0	dep	range	939:943	arg1	D50					946:948	D50	946:948	D50	946:948	The particle size remained in a relatively narrow range (D50, 2.16-3.67 μm), and therefore was considered independent of the factors investigated.
34919998	2	1	theme	spray	387:391	arg1	parameters					411:420	spray drying processing parameters	387:420	spray drying processing parameters	387:420	However, developing such formulations with desirable quality attributes is challenging due to the sensitivity of microparticle critical quality attributes towards changes in spray drying processing parameters.
34919998	8	2	theme	pectin	1185:1190	arg1	microparticles					1192:1205	the pectin microparticles	1181:1205	the pectin microparticles	1181:1205	The presence of octreotide acetate in the pectin microparticles increased their surface roughness and decreased their melting enthalpy.
34919998	6	3	theme	narrow	932:937	arg1	range					939:943	a relatively narrow range	919:943	a relatively narrow range (D50, 2.16-3.67 μm)	919:963	The particle size remained in a relatively narrow range (D50, 2.16-3.67 μm), and therefore was considered independent of the factors investigated.
34919998	3	4	theme	polymer	569:575	arg1	concentration					577:589	polymer concentration	569:589	polymer concentration	569:589	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	9	5	theme	lower	1430:1434	arg1	degree					1436:1441	a lower degree	1428:1441	a lower degree of esterification (62%, CU401)	1428:1472	In addition, it was determined that pectin with a high degree of esterification (72%, AU201) resulted in faster drug release compared to pectin with a lower degree of esterification (62%, CU401).
34919998	2	6	from	changes	376:382	arg1	parameters					411:420	spray drying processing parameters	387:420	spray drying processing parameters	387:420	However, developing such formulations with desirable quality attributes is challenging due to the sensitivity of microparticle critical quality attributes towards changes in spray drying processing parameters.
34919998	10	7	theme	particle	1548:1555	arg1	size					1557:1560	microparticle particle size	1534:1560	microparticle particle size	1534:1560	Interestingly, the degree of esterification did not impact microparticle particle size, morphology or thermal properties.
34919998	7	8	theme	representative	1119:1132	arg1	acetate					1106:1112	octreotide acetate	1095:1112	octreotide acetate	1095:1112	The model for yield prediction was further validated using octreotide acetate as a representative peptide.
34919998	7	8	theme	representative	1119:1132	arg1	peptide					1134:1140	a representative peptide	1117:1140	a representative peptide	1117:1140	The model for yield prediction was further validated using octreotide acetate as a representative peptide.
34919998	3	9	theme	rate	563:566	arg1	influence					505:513	the influence	501:513	the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying	501:695	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	8	10	theme	surface	1223:1229	arg1	roughness					1231:1239	their surface roughness	1217:1239	their surface roughness	1217:1239	The presence of octreotide acetate in the pectin microparticles increased their surface roughness and decreased their melting enthalpy.
34919998	13	11	theme	drug	1971:1974	arg1	products					1976:1983	similar drug products	1963:1983	similar drug products	1963:1983	The yield prediction model achieved using a central composite design may also be helpful for formulation development of similar drug products.
34919998	2	12	theme	critical	340:347	arg1	attributes					357:366	microparticle critical quality attributes	326:366	microparticle critical quality attributes	326:366	However, developing such formulations with desirable quality attributes is challenging due to the sensitivity of microparticle critical quality attributes towards changes in spray drying processing parameters.
34919998	1	13	theme	mucoadhesion	199:210	arg1	virtue					183:188	virtue	183:188	virtue of their mucoadhesion	183:210	Spray-dried pectin microparticles can potentially improve the oral bioavailability of peptides by virtue of their mucoadhesion.
34919998	7	14	theme	octreotide	1095:1104	arg1	acetate					1106:1112	octreotide acetate	1095:1112	octreotide acetate	1095:1112	The model for yield prediction was further validated using octreotide acetate as a representative peptide.
34919998	7	14	theme	octreotide	1095:1104	arg1	peptide					1134:1140	a representative peptide	1117:1140	a representative peptide	1117:1140	The model for yield prediction was further validated using octreotide acetate as a representative peptide.
34919998	3	15	theme	rate	547:550	arg1	influence					505:513	the influence	501:513	the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying	501:695	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	5	16	theme	spray	864:868	arg1	conditions					877:886	the spray drying conditions	860:886	the spray drying conditions	860:886	A maximum yield of 72.2% was achieved through optimizing the spray drying conditions.
34919998	4	17	with	significant	765:775	arg1	predictability					787:800	good predictability	782:800	good predictability	782:800	A mathematical model for the prediction of yield was statistically significant with good predictability.
34919998	2	18	theme	such	233:236	arg1	formulations					238:249	developing such formulations	222:249	developing such formulations with desirable quality attributes	222:283	However, developing such formulations with desirable quality attributes is challenging due to the sensitivity of microparticle critical quality attributes towards changes in spray drying processing parameters.
34919998	7	19	theme	yield	1050:1054	arg1	prediction					1056:1065	yield prediction	1050:1065	yield prediction	1050:1065	The model for yield prediction was further validated using octreotide acetate as a representative peptide.
34919998	1	20	theme	oral	147:150	arg1	bioavailability					152:166	the oral bioavailability	143:166	the oral bioavailability of peptides	143:178	Spray-dried pectin microparticles can potentially improve the oral bioavailability of peptides by virtue of their mucoadhesion.
34919998	11	21	theme	microparticles	1731:1744	arg1	development					1695:1705	the development	1691:1705	the development of pectin-based peptide microparticles	1691:1744	This study demonstrates the importance of DoE in optimization of the spray drying process for the development of pectin-based peptide microparticles.
34919998	0	22	theme	spray	60:64	arg1	processing					73:82	spray drying processing	60:82	spray drying processing	60:82	Pectin microparticles for peptide delivery: Optimization of spray drying processing.
34919998	3	23	theme	particle	632:639	arg1	size					641:644	particle size	632:644	particle size	632:644	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	0	24	theme	processing	73:82	arg1	Optimization					44:55	Optimization	44:55	Pectin microparticles for peptide delivery: Optimization of spray drying processing.	0:83	Pectin microparticles for peptide delivery: Optimization of spray drying processing.
34919998	9	25	theme	esterification	1344:1357	arg1	degree					1334:1339	a high degree	1327:1339	a high degree of esterification (72%, AU201)	1327:1370	In addition, it was determined that pectin with a high degree of esterification (72%, AU201) resulted in faster drug release compared to pectin with a lower degree of esterification (62%, CU401).
34919998	11	26	from	importance	1625:1634	arg1	optimization					1646:1657	optimization	1646:1657	optimization of the spray drying process	1646:1685	This study demonstrates the importance of DoE in optimization of the spray drying process for the development of pectin-based peptide microparticles.
34919998	9	27	dep	resulted	1372:1379	arg1	compared					1404:1411	compared	1404:1411	compared to pectin with a lower degree of esterification (62%, CU401)	1404:1472	In addition, it was determined that pectin with a high degree of esterification (72%, AU201) resulted in faster drug release compared to pectin with a lower degree of esterification (62%, CU401).
34919998	13	28	theme	composite	1895:1903	arg1	design					1905:1910	a central composite design	1885:1910	a central composite design	1885:1910	The yield prediction model achieved using a central composite design may also be helpful for formulation development of similar drug products.
34919998	3	29	theme	design	458:463	arg1	approach					465:472	a central composite design approach	438:472	a central composite design approach	438:472	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	0	30	theme	Pectin	0:5	arg1	microparticles					7:20	Pectin microparticles	0:20	Pectin microparticles for peptide delivery: Optimization of spray drying processing.	0:83	Pectin microparticles for peptide delivery: Optimization of spray drying processing.
34919998	1	31	theme	pectin	97:102	arg1	microparticles					104:117	Spray-dried pectin microparticles	85:117	Spray-dried pectin microparticles	85:117	Spray-dried pectin microparticles can potentially improve the oral bioavailability of peptides by virtue of their mucoadhesion.
34919998	4	32	theme	good	782:785	arg1	predictability					787:800	good predictability	782:800	good predictability	782:800	A mathematical model for the prediction of yield was statistically significant with good predictability.
34919998	13	33	theme	prediction	1853:1862	arg1	model					1864:1868	The yield prediction model	1843:1868	The yield prediction model achieved using a central composite design	1843:1910	The yield prediction model achieved using a central composite design may also be helpful for formulation development of similar drug products.
34919998	13	33	theme	prediction	1853:1862	arg1	helpful					1924:1930	helpful	1924:1930	helpful	1924:1930	The yield prediction model achieved using a central composite design may also be helpful for formulation development of similar drug products.
34919998	3	34	theme	central	440:446	arg1	approach					465:472	a central composite design approach	438:472	a central composite design approach	438:472	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	10	35	theme	esterification	1504:1517	arg1	degree					1494:1499	the degree	1490:1499	the degree of esterification	1490:1517	Interestingly, the degree of esterification did not impact microparticle particle size, morphology or thermal properties.
34919998	3	36	theme	microparticles	656:669	arg1	yield					622:626	yield	622:626	yield	622:626	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	3	36	theme	microparticles	656:669	arg1	size					641:644	particle size	632:644	particle size	632:644	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	9	37	dep	degree	1436:1441	arg1	%					1464:1464	62%	1462:1464	62%	1462:1464	In addition, it was determined that pectin with a high degree of esterification (72%, AU201) resulted in faster drug release compared to pectin with a lower degree of esterification (62%, CU401).
34919998	9	37	dep	degree	1436:1441	arg1	CU401					1467:1471	CU401	1467:1471	CU401	1467:1471	In addition, it was determined that pectin with a high degree of esterification (72%, AU201) resulted in faster drug release compared to pectin with a lower degree of esterification (62%, CU401).
34919998	3	38	theme	temperature	524:534	arg1	influence					505:513	the influence	501:513	the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying	501:695	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	5	39	theme	%	826:826	arg1	yield					813:817	A maximum yield	803:817	A maximum yield of 72.2%	803:826	A maximum yield of 72.2% was achieved through optimizing the spray drying conditions.
34919998	11	40	theme	drying	1672:1677	arg1	process					1679:1685	the spray drying process	1662:1685	the spray drying process	1662:1685	This study demonstrates the importance of DoE in optimization of the spray drying process for the development of pectin-based peptide microparticles.
34919998	2	41	theme	quality	266:272	arg1	attributes					274:283	desirable quality attributes	256:283	desirable quality attributes	256:283	However, developing such formulations with desirable quality attributes is challenging due to the sensitivity of microparticle critical quality attributes towards changes in spray drying processing parameters.
34919998	11	42	theme	peptide	1723:1729	arg1	microparticles					1731:1744	pectin-based peptide microparticles	1710:1744	pectin-based peptide microparticles	1710:1744	This study demonstrates the importance of DoE in optimization of the spray drying process for the development of pectin-based peptide microparticles.
34919998	3	43	from	influence	505:513	arg1	yield					622:626	yield	622:626	yield	622:626	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	3	43	from	influence	505:513	arg1	size					641:644	particle size	632:644	particle size	632:644	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	9	44	theme	drug	1391:1394	arg1	release					1396:1402	faster drug release	1384:1402	faster drug release	1384:1402	In addition, it was determined that pectin with a high degree of esterification (72%, AU201) resulted in faster drug release compared to pectin with a lower degree of esterification (62%, CU401).
34919998	12	45	theme	pectin-based	1790:1801	arg1	microparticles					1803:1816	spray-dried pectin-based microparticles	1778:1816	spray-dried pectin-based microparticles for octreotide delivery	1778:1840	This is the first report using spray-dried pectin-based microparticles for octreotide delivery.
34919998	10	46	theme	microparticle	1534:1546	arg1	size					1557:1560	microparticle particle size	1534:1560	microparticle particle size	1534:1560	Interestingly, the degree of esterification did not impact microparticle particle size, morphology or thermal properties.
34919998	11	47	theme	process	1679:1685	arg1	optimization					1646:1657	optimization	1646:1657	optimization of the spray drying process	1646:1685	This study demonstrates the importance of DoE in optimization of the spray drying process for the development of pectin-based peptide microparticles.
34919998	2	48	theme	drying	393:398	arg1	parameters					411:420	spray drying processing parameters	387:420	spray drying processing parameters	387:420	However, developing such formulations with desirable quality attributes is challenging due to the sensitivity of microparticle critical quality attributes towards changes in spray drying processing parameters.
34919998	8	49	theme	acetate	1170:1176	arg1	presence					1147:1154	The presence	1143:1154	The presence of octreotide acetate in the pectin microparticles	1143:1205	The presence of octreotide acetate in the pectin microparticles increased their surface roughness and decreased their melting enthalpy.
34919998	12	50	theme	first	1759:1763	arg1	This					1747:1750	This	1747:1750	This	1747:1750	This is the first report using spray-dried pectin-based microparticles for octreotide delivery.
34919998	12	50	theme	first	1759:1763	arg1	report					1765:1770	the first report	1755:1770	the first report using spray-dried pectin-based microparticles for octreotide delivery	1755:1840	This is the first report using spray-dried pectin-based microparticles for octreotide delivery.
34919998	8	51	attach	presence	1147:1154	arg1	microparticles					1192:1205	the pectin microparticles	1181:1205	the pectin microparticles	1181:1205	The presence of octreotide acetate in the pectin microparticles increased their surface roughness and decreased their melting enthalpy.
34919998	8	51	attach	presence	1147:1154	arg2	acetate					1170:1176	octreotide acetate	1159:1176	octreotide acetate	1159:1176	The presence of octreotide acetate in the pectin microparticles increased their surface roughness and decreased their melting enthalpy.
34919998	3	52	theme	polymer	595:601	arg1	weight					608:613	polymer feed weight	595:613	polymer feed weight	595:613	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	11	53	theme	spray	1666:1670	arg1	process					1679:1685	the spray drying process	1662:1685	the spray drying process	1662:1685	This study demonstrates the importance of DoE in optimization of the spray drying process for the development of pectin-based peptide microparticles.
34919998	3	54	theme	flow	558:561	arg1	rate					563:566	feed flow rate	553:566	feed flow rate	553:566	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	8	55	from	presence	1147:1154	arg1	microparticles					1192:1205	the pectin microparticles	1181:1205	the pectin microparticles	1181:1205	The presence of octreotide acetate in the pectin microparticles increased their surface roughness and decreased their melting enthalpy.
34919998	5	56	theme	maximum	805:811	arg1	yield					813:817	A maximum yield	803:817	A maximum yield of 72.2%	803:826	A maximum yield of 72.2% was achieved through optimizing the spray drying conditions.
34919998	13	57	theme	similar	1963:1969	arg1	products					1976:1983	similar drug products	1963:1983	similar drug products	1963:1983	The yield prediction model achieved using a central composite design may also be helpful for formulation development of similar drug products.
34919998	2	58	theme	quality	349:355	arg1	attributes					357:366	microparticle critical quality attributes	326:366	microparticle critical quality attributes	326:366	However, developing such formulations with desirable quality attributes is challenging due to the sensitivity of microparticle critical quality attributes towards changes in spray drying processing parameters.
34919998	9	59	theme	esterification	1446:1459	arg1	degree					1436:1441	a lower degree	1428:1441	a lower degree of esterification (62%, CU401)	1428:1472	In addition, it was determined that pectin with a high degree of esterification (72%, AU201) resulted in faster drug release compared to pectin with a lower degree of esterification (62%, CU401).
34919998	2	60	with	formulations	238:249	arg1	attributes					274:283	desirable quality attributes	256:283	desirable quality attributes	256:283	However, developing such formulations with desirable quality attributes is challenging due to the sensitivity of microparticle critical quality attributes towards changes in spray drying processing parameters.
34919998	11	61	theme	pectin-based	1710:1721	arg1	microparticles					1731:1744	pectin-based peptide microparticles	1710:1744	pectin-based peptide microparticles	1710:1744	This study demonstrates the importance of DoE in optimization of the spray drying process for the development of pectin-based peptide microparticles.
34919998	10	62	theme	thermal	1577:1583	arg1	properties					1585:1594	thermal properties	1577:1594	thermal properties	1577:1594	Interestingly, the degree of esterification did not impact microparticle particle size, morphology or thermal properties.
34919998	13	63	theme	products	1976:1983	arg1	development					1948:1958	formulation development	1936:1958	formulation development of similar drug products	1936:1983	The yield prediction model achieved using a central composite design may also be helpful for formulation development of similar drug products.
34919998	2	64	theme	microparticle	326:338	arg1	attributes					357:366	microparticle critical quality attributes	326:366	microparticle critical quality attributes	326:366	However, developing such formulations with desirable quality attributes is challenging due to the sensitivity of microparticle critical quality attributes towards changes in spray drying processing parameters.
34919998	3	65	theme	feed	603:606	arg1	weight					608:613	polymer feed weight	595:613	polymer feed weight	595:613	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	3	66	theme	aspirator	537:545	arg1	rate					547:550	aspirator rate	537:550	aspirator rate	537:550	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	2	67	theme	desirable	256:264	arg1	attributes					274:283	desirable quality attributes	256:283	desirable quality attributes	256:283	However, developing such formulations with desirable quality attributes is challenging due to the sensitivity of microparticle critical quality attributes towards changes in spray drying processing parameters.
34919998	3	68	dep	yield	622:626	arg1	the					618:620	the	618:620	the	618:620	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	5	69	theme	drying	870:875	arg1	conditions					877:886	the spray drying conditions	860:886	the spray drying conditions	860:886	A maximum yield of 72.2% was achieved through optimizing the spray drying conditions.
34919998	3	70	theme	weight	608:613	arg1	influence					505:513	the influence	501:513	the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying	501:695	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	8	71	theme	melting	1261:1267	arg1	enthalpy					1269:1276	their melting enthalpy	1255:1276	their melting enthalpy	1255:1276	The presence of octreotide acetate in the pectin microparticles increased their surface roughness and decreased their melting enthalpy.
34919998	3	72	theme	concentration	577:589	arg1	influence					505:513	the influence	501:513	the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying	501:695	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	4	73	theme	yield	741:745	arg1	prediction					727:736	the prediction	723:736	the prediction of yield	723:745	A mathematical model for the prediction of yield was statistically significant with good predictability.
34919998	13	74	theme	formulation	1936:1946	arg1	development					1948:1958	formulation development	1936:1958	formulation development of similar drug products	1936:1983	The yield prediction model achieved using a central composite design may also be helpful for formulation development of similar drug products.
34919998	0	75	theme	drying	66:71	arg1	processing					73:82	spray drying processing	60:82	spray drying processing	60:82	Pectin microparticles for peptide delivery: Optimization of spray drying processing.
34919998	4	76	theme	mathematical	700:711	arg1	model					713:717	A mathematical model	698:717	A mathematical model for the prediction of yield	698:745	A mathematical model for the prediction of yield was statistically significant with good predictability.
34919998	4	76	theme	mathematical	700:711	arg1	significant					765:775	significant	765:775	significant	765:775	A mathematical model for the prediction of yield was statistically significant with good predictability.
34919998	1	77	theme	peptides	171:178	arg1	bioavailability					152:166	the oral bioavailability	143:166	the oral bioavailability of peptides	143:178	Spray-dried pectin microparticles can potentially improve the oral bioavailability of peptides by virtue of their mucoadhesion.
34919998	6	78	theme	factors	1014:1020	arg1	independent					995:1005	independent	995:1005	independent	995:1005	The particle size remained in a relatively narrow range (D50, 2.16-3.67 μm), and therefore was considered independent of the factors investigated.
34919998	9	79	with	pectin	1315:1320	arg1	degree					1334:1339	a high degree	1327:1339	a high degree of esterification (72%, AU201)	1327:1370	In addition, it was determined that pectin with a high degree of esterification (72%, AU201) resulted in faster drug release compared to pectin with a lower degree of esterification (62%, CU401).
34919998	9	80	theme	high	1329:1332	arg1	degree					1334:1339	a high degree	1327:1339	a high degree of esterification (72%, AU201)	1327:1370	In addition, it was determined that pectin with a high degree of esterification (72%, AU201) resulted in faster drug release compared to pectin with a lower degree of esterification (62%, CU401).
34919998	3	81	theme	input	518:522	arg1	temperature					524:534	input temperature	518:534	input temperature	518:534	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	13	82	theme	central	1887:1893	arg1	design					1905:1910	a central composite design	1885:1910	a central composite design	1885:1910	The yield prediction model achieved using a central composite design may also be helpful for formulation development of similar drug products.
34919998	1	83	theme	Spray-dried	85:95	arg1	microparticles					104:117	Spray-dried pectin microparticles	85:117	Spray-dried pectin microparticles	85:117	Spray-dried pectin microparticles can potentially improve the oral bioavailability of peptides by virtue of their mucoadhesion.
34919998	13	84	theme	yield	1847:1851	arg1	model					1864:1868	The yield prediction model	1843:1868	The yield prediction model achieved using a central composite design	1843:1910	The yield prediction model achieved using a central composite design may also be helpful for formulation development of similar drug products.
34919998	13	84	theme	yield	1847:1851	arg1	helpful					1924:1930	helpful	1924:1930	helpful	1924:1930	The yield prediction model achieved using a central composite design may also be helpful for formulation development of similar drug products.
34919998	0	85	theme	peptide	26:32	arg1	delivery					34:41	peptide delivery	26:41	peptide delivery	26:41	Pectin microparticles for peptide delivery: Optimization of spray drying processing.
34919998	6	86	theme	particle	893:900	arg1	size					902:905	The particle size	889:905	The particle size	889:905	The particle size remained in a relatively narrow range (D50, 2.16-3.67 μm), and therefore was considered independent of the factors investigated.
34919998	3	87	theme	composite	448:456	arg1	approach					465:472	a central composite design approach	438:472	a central composite design approach	438:472	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	9	88	dep	esterification	1344:1357	arg1	AU201					1365:1369	AU201	1365:1369	AU201	1365:1369	In addition, it was determined that pectin with a high degree of esterification (72%, AU201) resulted in faster drug release compared to pectin with a lower degree of esterification (62%, CU401).
34919998	9	88	dep	esterification	1344:1357	arg1	%					1362:1362	72%	1360:1362	72%	1360:1362	In addition, it was determined that pectin with a high degree of esterification (72%, AU201) resulted in faster drug release compared to pectin with a lower degree of esterification (62%, CU401).
34919998	3	89	theme	pectin	649:654	arg1	microparticles					656:669	pectin microparticles	649:669	pectin microparticles prepared via spray drying	649:695	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	12	90	theme	octreotide	1822:1831	arg1	delivery					1833:1840	octreotide delivery	1822:1840	octreotide delivery	1822:1840	This is the first report using spray-dried pectin-based microparticles for octreotide delivery.
34919998	2	91	theme	processing	400:409	arg1	parameters					411:420	spray drying processing parameters	387:420	spray drying processing parameters	387:420	However, developing such formulations with desirable quality attributes is challenging due to the sensitivity of microparticle critical quality attributes towards changes in spray drying processing parameters.
34919998	2	92	theme	developing	222:231	arg1	formulations					238:249	developing such formulations	222:249	developing such formulations with desirable quality attributes	222:283	However, developing such formulations with desirable quality attributes is challenging due to the sensitivity of microparticle critical quality attributes towards changes in spray drying processing parameters.
34919998	9	93	theme	faster	1384:1389	arg1	release					1396:1402	faster drug release	1384:1402	faster drug release	1384:1402	In addition, it was determined that pectin with a high degree of esterification (72%, AU201) resulted in faster drug release compared to pectin with a lower degree of esterification (62%, CU401).
34919998	3	94	theme	spray	684:688	arg1	drying					690:695	spray drying	684:695	spray drying	684:695	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34919998	2	95	theme	attributes	357:366	arg1	sensitivity					311:321	the sensitivity	307:321	the sensitivity of microparticle critical quality attributes towards changes in spray drying processing parameters	307:420	However, developing such formulations with desirable quality attributes is challenging due to the sensitivity of microparticle critical quality attributes towards changes in spray drying processing parameters.
34919998	8	96	theme	octreotide	1159:1168	arg1	acetate					1170:1176	octreotide acetate	1159:1176	octreotide acetate	1159:1176	The presence of octreotide acetate in the pectin microparticles increased their surface roughness and decreased their melting enthalpy.
34919998	0	97	dep	microparticles	7:20	arg1	Optimization					44:55	Optimization	44:55	Pectin microparticles for peptide delivery: Optimization of spray drying processing.	0:83	Pectin microparticles for peptide delivery: Optimization of spray drying processing.
34919998	12	98	theme	spray-dried	1778:1788	arg1	microparticles					1803:1816	spray-dried pectin-based microparticles	1778:1816	spray-dried pectin-based microparticles for octreotide delivery	1778:1840	This is the first report using spray-dried pectin-based microparticles for octreotide delivery.
34919998	11	99	theme	DoE	1639:1641	arg1	importance					1625:1634	the importance	1621:1634	the importance of DoE in optimization of the spray drying process	1621:1685	This study demonstrates the importance of DoE in optimization of the spray drying process for the development of pectin-based peptide microparticles.
34919998	3	100	theme	feed	553:556	arg1	rate					563:566	feed flow rate	553:566	feed flow rate	553:566	In this study, a central composite design approach was applied to investigate the influence of input temperature, aspirator rate, feed flow rate, polymer concentration and polymer feed weight on the yield and particle size of pectin microparticles prepared via spray drying.
34271053	2	0	theme	components	479:488	arg1	modification					448:459	desired modification	440:459	desired modification of other chemical components	440:488	The investigation of chemical constituents of optimally alkalized SISF revealed that ideal increment of cellulose content (68.69 wt%) and desired modification of other chemical components was accomplished through 60 min immersion period.
34271053	2	0	theme	components	479:488	arg1	increment					393:401	ideal increment	387:401	ideal increment of cellulose content (68.69 wt%)	387:434	The investigation of chemical constituents of optimally alkalized SISF revealed that ideal increment of cellulose content (68.69 wt%) and desired modification of other chemical components was accomplished through 60 min immersion period.
34271053	5	1	theme	tensile	917:923	arg1	471.2 ± 19.8 MPa					935:950	471.2 ± 19.8 MPa	935:950	471.2 ± 19.8 MPa	935:950	The enhancements in tensile strength (471.2 ± 19.8 MPa), tensile modulus (5.82 ± 0.77 GPa) and thermal stability (371 °C) were noted that ensures the treated fiber has good mechanical and thermal properties required for composite preparation.
34271053	5	1	theme	tensile	917:923	arg1	strength					925:932	tensile strength	917:932	tensile strength (471.2 ± 19.8 MPa)	917:951	The enhancements in tensile strength (471.2 ± 19.8 MPa), tensile modulus (5.82 ± 0.77 GPa) and thermal stability (371 °C) were noted that ensures the treated fiber has good mechanical and thermal properties required for composite preparation.
34271053	1	2	theme	surface	237:243	arg1	properties					245:254	surface properties	237:254	surface properties	237:254	The present study investigated the effect of alkali treatment on the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF).
34271053	0	3	theme	natural	64:70	arg1	fibers					72:77	natural fibers	64:77	natural fibers from Symphirema involucratum stem	64:111	Comprehensive characterization of raw and alkali (NaOH) treated natural fibers from Symphirema involucratum stem.
34271053	4	4	theme	weight	878:883	arg1	percentage					885:894	total weight percentage	872:894	total weight percentage	872:894	Moreover, the treated fiber was found suitable for light-weight applications since physical analysis acknowledges that the density of the fiber augmented to 1424 kg/m3after surface treatment that reduces total weight percentage.
34271053	1	5	from	effect	149:154	arg1	enhancement					183:193	the enhancement	179:193	the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF)	179:299	The present study investigated the effect of alkali treatment on the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF).
34271053	4	6	theme	treated	682:688	arg1	fiber					690:694	the treated fiber	678:694	the treated fiber	678:694	Moreover, the treated fiber was found suitable for light-weight applications since physical analysis acknowledges that the density of the fiber augmented to 1424 kg/m3after surface treatment that reduces total weight percentage.
34271053	3	7	from	increase	543:550	arg1	index					573:577	the crystallinity index	555:577	the crystallinity index to 33.33% and small crystallite size to 3.21 nm	555:625	An increase in the crystallinity index to 33.33% and small crystallite size to 3.21 nm was noted by X-ray diffraction analysis.
34271053	1	8	theme	properties	245:254	arg1	enhancement					183:193	the enhancement	179:193	the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF)	179:299	The present study investigated the effect of alkali treatment on the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF).
34271053	0	9	theme	Symphirema	84:93	arg1	stem					108:111	Symphirema involucratum stem	84:111	Symphirema involucratum stem	84:111	Comprehensive characterization of raw and alkali (NaOH) treated natural fibers from Symphirema involucratum stem.
34271053	6	10	theme	suitable	1211:1218	arg1	material					1220:1227	a suitable material	1209:1227	a suitable material for lightweight composite structures	1209:1264	These findings validated that the optimally surface-modified SISF is a suitable material for lightweight composite structures, for the time being.
34271053	6	10	theme	suitable	1211:1218	arg1	SISF					1201:1204	the optimally surface-modified SISF	1170:1204	the optimally surface-modified SISF	1170:1204	These findings validated that the optimally surface-modified SISF is a suitable material for lightweight composite structures, for the time being.
34271053	2	11	theme	immersion	522:530	arg1	period					532:537	60 min immersion period	515:537	60 min immersion period	515:537	The investigation of chemical constituents of optimally alkalized SISF revealed that ideal increment of cellulose content (68.69 wt%) and desired modification of other chemical components was accomplished through 60 min immersion period.
34271053	5	12	from	enhancements	901:912	arg1	471.2 ± 19.8 MPa					935:950	471.2 ± 19.8 MPa	935:950	471.2 ± 19.8 MPa	935:950	The enhancements in tensile strength (471.2 ± 19.8 MPa), tensile modulus (5.82 ± 0.77 GPa) and thermal stability (371 °C) were noted that ensures the treated fiber has good mechanical and thermal properties required for composite preparation.
34271053	5	12	from	enhancements	901:912	arg1	stability					1000:1008	thermal stability	992:1008	thermal stability (371 °C)	992:1017	The enhancements in tensile strength (471.2 ± 19.8 MPa), tensile modulus (5.82 ± 0.77 GPa) and thermal stability (371 °C) were noted that ensures the treated fiber has good mechanical and thermal properties required for composite preparation.
34271053	5	12	from	enhancements	901:912	arg1	strength					925:932	tensile strength	917:932	tensile strength (471.2 ± 19.8 MPa)	917:951	The enhancements in tensile strength (471.2 ± 19.8 MPa), tensile modulus (5.82 ± 0.77 GPa) and thermal stability (371 °C) were noted that ensures the treated fiber has good mechanical and thermal properties required for composite preparation.
34271053	5	12	from	enhancements	901:912	arg1	modulus					962:968	tensile modulus	954:968	tensile modulus (5.82 ± 0.77 GPa)	954:986	The enhancements in tensile strength (471.2 ± 19.8 MPa), tensile modulus (5.82 ± 0.77 GPa) and thermal stability (371 °C) were noted that ensures the treated fiber has good mechanical and thermal properties required for composite preparation.
34271053	5	12	from	enhancements	901:912	arg1	5.82 ± 0.77 GPa					971:985	5.82 ± 0.77 GPa	971:985	5.82 ± 0.77 GPa	971:985	The enhancements in tensile strength (471.2 ± 19.8 MPa), tensile modulus (5.82 ± 0.77 GPa) and thermal stability (371 °C) were noted that ensures the treated fiber has good mechanical and thermal properties required for composite preparation.
34271053	5	12	from	enhancements	901:912	arg1	371 °C					1011:1016	371 °C	1011:1016	371 °C	1011:1016	The enhancements in tensile strength (471.2 ± 19.8 MPa), tensile modulus (5.82 ± 0.77 GPa) and thermal stability (371 °C) were noted that ensures the treated fiber has good mechanical and thermal properties required for composite preparation.
34271053	1	13	theme	Symphirema	259:268	arg1	fiber					288:292	Symphirema involucratum stem fiber	259:292	Symphirema involucratum stem fiber (SISF)	259:299	The present study investigated the effect of alkali treatment on the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF).
34271053	1	13	theme	Symphirema	259:268	arg1	SISF					295:298	SISF	295:298	SISF	295:298	The present study investigated the effect of alkali treatment on the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF).
34271053	2	14	theme	60 min	515:520	arg1	period					532:537	60 min immersion period	515:537	60 min immersion period	515:537	The investigation of chemical constituents of optimally alkalized SISF revealed that ideal increment of cellulose content (68.69 wt%) and desired modification of other chemical components was accomplished through 60 min immersion period.
34271053	5	15	theme	thermal	992:998	arg1	371 °C					1011:1016	371 °C	1011:1016	371 °C	1011:1016	The enhancements in tensile strength (471.2 ± 19.8 MPa), tensile modulus (5.82 ± 0.77 GPa) and thermal stability (371 °C) were noted that ensures the treated fiber has good mechanical and thermal properties required for composite preparation.
34271053	5	15	theme	thermal	992:998	arg1	stability					1000:1008	thermal stability	992:1008	thermal stability (371 °C)	992:1017	The enhancements in tensile strength (471.2 ± 19.8 MPa), tensile modulus (5.82 ± 0.77 GPa) and thermal stability (371 °C) were noted that ensures the treated fiber has good mechanical and thermal properties required for composite preparation.
34271053	1	16	theme	alkali	159:164	arg1	treatment					166:174	alkali treatment	159:174	alkali treatment	159:174	The present study investigated the effect of alkali treatment on the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF).
34271053	0	17	theme	involucratum	95:106	arg1	stem					108:111	Symphirema involucratum stem	84:111	Symphirema involucratum stem	84:111	Comprehensive characterization of raw and alkali (NaOH) treated natural fibers from Symphirema involucratum stem.
34271053	5	18	theme	composite	1117:1125	arg1	preparation					1127:1137	composite preparation	1117:1137	composite preparation	1117:1137	The enhancements in tensile strength (471.2 ± 19.8 MPa), tensile modulus (5.82 ± 0.77 GPa) and thermal stability (371 °C) were noted that ensures the treated fiber has good mechanical and thermal properties required for composite preparation.
34271053	2	19	theme	other	464:468	arg1	components					479:488	other chemical components	464:488	other chemical components	464:488	The investigation of chemical constituents of optimally alkalized SISF revealed that ideal increment of cellulose content (68.69 wt%) and desired modification of other chemical components was accomplished through 60 min immersion period.
34271053	1	20	theme	treatment	166:174	arg1	effect					149:154	the effect	145:154	the effect of alkali treatment on the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF)	145:299	The present study investigated the effect of alkali treatment on the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF).
34271053	2	21	theme	chemical	470:477	arg1	components					479:488	other chemical components	464:488	other chemical components	464:488	The investigation of chemical constituents of optimally alkalized SISF revealed that ideal increment of cellulose content (68.69 wt%) and desired modification of other chemical components was accomplished through 60 min immersion period.
34271053	4	22	theme	total	872:876	arg1	percentage					885:894	total weight percentage	872:894	total weight percentage	872:894	Moreover, the treated fiber was found suitable for light-weight applications since physical analysis acknowledges that the density of the fiber augmented to 1424 kg/m3after surface treatment that reduces total weight percentage.
34271053	5	23	theme	tensile	954:960	arg1	5.82 ± 0.77 GPa					971:985	5.82 ± 0.77 GPa	971:985	5.82 ± 0.77 GPa	971:985	The enhancements in tensile strength (471.2 ± 19.8 MPa), tensile modulus (5.82 ± 0.77 GPa) and thermal stability (371 °C) were noted that ensures the treated fiber has good mechanical and thermal properties required for composite preparation.
34271053	5	23	theme	tensile	954:960	arg1	modulus					962:968	tensile modulus	954:968	tensile modulus (5.82 ± 0.77 GPa)	954:986	The enhancements in tensile strength (471.2 ± 19.8 MPa), tensile modulus (5.82 ± 0.77 GPa) and thermal stability (371 °C) were noted that ensures the treated fiber has good mechanical and thermal properties required for composite preparation.
34271053	0	24	theme	Comprehensive	0:12	arg1	characterization					14:29	Comprehensive characterization	0:29	Comprehensive characterization of raw and alkali (NaOH)	0:54	Comprehensive characterization of raw and alkali (NaOH) treated natural fibers from Symphirema involucratum stem.
34271053	1	25	theme	involucratum	270:281	arg1	fiber					288:292	Symphirema involucratum stem fiber	259:292	Symphirema involucratum stem fiber (SISF)	259:299	The present study investigated the effect of alkali treatment on the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF).
34271053	1	25	theme	involucratum	270:281	arg1	SISF					295:298	SISF	295:298	SISF	295:298	The present study investigated the effect of alkali treatment on the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF).
34271053	4	26	theme	light-weight	719:730	arg1	applications					732:743	light-weight applications	719:743	light-weight applications	719:743	Moreover, the treated fiber was found suitable for light-weight applications since physical analysis acknowledges that the density of the fiber augmented to 1424 kg/m3after surface treatment that reduces total weight percentage.
34271053	6	27	theme	surface-modified	1184:1199	arg1	material					1220:1227	a suitable material	1209:1227	a suitable material for lightweight composite structures	1209:1264	These findings validated that the optimally surface-modified SISF is a suitable material for lightweight composite structures, for the time being.
34271053	6	27	theme	surface-modified	1184:1199	arg1	SISF					1201:1204	the optimally surface-modified SISF	1170:1204	the optimally surface-modified SISF	1170:1204	These findings validated that the optimally surface-modified SISF is a suitable material for lightweight composite structures, for the time being.
34271053	3	28	theme	X-ray	640:644	arg1	analysis					658:665	X-ray diffraction analysis	640:665	X-ray diffraction analysis	640:665	An increase in the crystallinity index to 33.33% and small crystallite size to 3.21 nm was noted by X-ray diffraction analysis.
34271053	0	29	theme	raw	34:36	arg1	characterization					14:29	Comprehensive characterization	0:29	Comprehensive characterization of raw and alkali (NaOH)	0:54	Comprehensive characterization of raw and alkali (NaOH) treated natural fibers from Symphirema involucratum stem.
34271053	1	30	theme	stem	283:286	arg1	fiber					288:292	Symphirema involucratum stem fiber	259:292	Symphirema involucratum stem fiber (SISF)	259:299	The present study investigated the effect of alkali treatment on the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF).
34271053	1	30	theme	stem	283:286	arg1	SISF					295:298	SISF	295:298	SISF	295:298	The present study investigated the effect of alkali treatment on the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF).
34271053	4	31	theme	fiber	806:810	arg1	density					791:797	the density	787:797	the density of the fiber	787:810	Moreover, the treated fiber was found suitable for light-weight applications since physical analysis acknowledges that the density of the fiber augmented to 1424 kg/m3after surface treatment that reduces total weight percentage.
34271053	4	32	theme	physical	751:758	arg1	analysis					760:767	physical analysis	751:767	physical analysis	751:767	Moreover, the treated fiber was found suitable for light-weight applications since physical analysis acknowledges that the density of the fiber augmented to 1424 kg/m3after surface treatment that reduces total weight percentage.
34271053	1	33	theme	fiber	288:292	arg1	Physico-chemical					198:213	Physico-chemical	198:213	Physico-chemical	198:213	The present study investigated the effect of alkali treatment on the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF).
34271053	5	34	theme	mechanical	1070:1079	arg1	properties					1093:1102	good mechanical and thermal properties	1065:1102	good mechanical and thermal properties required for composite preparation	1065:1137	The enhancements in tensile strength (471.2 ± 19.8 MPa), tensile modulus (5.82 ± 0.77 GPa) and thermal stability (371 °C) were noted that ensures the treated fiber has good mechanical and thermal properties required for composite preparation.
34271053	3	35	theme	crystallinity	559:571	arg1	index					573:577	the crystallinity index	555:577	the crystallinity index to 33.33% and small crystallite size to 3.21 nm	555:625	An increase in the crystallinity index to 33.33% and small crystallite size to 3.21 nm was noted by X-ray diffraction analysis.
34271053	5	36	dep	fiber	1055:1059	arg1	has					1061:1063	has	1061:1063	has good mechanical and thermal properties required for composite preparation	1061:1137	The enhancements in tensile strength (471.2 ± 19.8 MPa), tensile modulus (5.82 ± 0.77 GPa) and thermal stability (371 °C) were noted that ensures the treated fiber has good mechanical and thermal properties required for composite preparation.
34271053	2	37	theme	content	416:422	arg1	modification					448:459	desired modification	440:459	desired modification of other chemical components	440:488	The investigation of chemical constituents of optimally alkalized SISF revealed that ideal increment of cellulose content (68.69 wt%) and desired modification of other chemical components was accomplished through 60 min immersion period.
34271053	2	37	theme	content	416:422	arg1	increment					393:401	ideal increment	387:401	ideal increment of cellulose content (68.69 wt%)	387:434	The investigation of chemical constituents of optimally alkalized SISF revealed that ideal increment of cellulose content (68.69 wt%) and desired modification of other chemical components was accomplished through 60 min immersion period.
34271053	0	38	theme	alkali	42:47	arg1	characterization					14:29	Comprehensive characterization	0:29	Comprehensive characterization of raw and alkali (NaOH)	0:54	Comprehensive characterization of raw and alkali (NaOH) treated natural fibers from Symphirema involucratum stem.
34271053	1	39	theme	Physico-chemical	198:213	arg1	enhancement					183:193	the enhancement	179:193	the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF)	179:299	The present study investigated the effect of alkali treatment on the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF).
34271053	5	40	theme	treated	1047:1053	arg1	fiber					1055:1059	the treated fiber	1043:1059	the treated fiber has good mechanical and thermal properties required for composite preparation	1043:1137	The enhancements in tensile strength (471.2 ± 19.8 MPa), tensile modulus (5.82 ± 0.77 GPa) and thermal stability (371 °C) were noted that ensures the treated fiber has good mechanical and thermal properties required for composite preparation.
34271053	2	41	theme	cellulose	406:414	arg1	content					416:422	cellulose content	406:422	cellulose content (68.69 wt%)	406:434	The investigation of chemical constituents of optimally alkalized SISF revealed that ideal increment of cellulose content (68.69 wt%) and desired modification of other chemical components was accomplished through 60 min immersion period.
34271053	2	41	theme	cellulose	406:414	arg1	%					433:433	68.69 wt%	425:433	68.69 wt%	425:433	The investigation of chemical constituents of optimally alkalized SISF revealed that ideal increment of cellulose content (68.69 wt%) and desired modification of other chemical components was accomplished through 60 min immersion period.
34271053	5	42	theme	thermal	1085:1091	arg1	properties					1093:1102	good mechanical and thermal properties	1065:1102	good mechanical and thermal properties required for composite preparation	1065:1137	The enhancements in tensile strength (471.2 ± 19.8 MPa), tensile modulus (5.82 ± 0.77 GPa) and thermal stability (371 °C) were noted that ensures the treated fiber has good mechanical and thermal properties required for composite preparation.
34271053	3	43	theme	small	593:597	arg1	size					611:614	small crystallite size	593:614	small crystallite size	593:614	An increase in the crystallinity index to 33.33% and small crystallite size to 3.21 nm was noted by X-ray diffraction analysis.
34271053	4	44	theme	surface	841:847	arg1	treatment					849:857	surface treatment	841:857	surface treatment that reduces total weight percentage	841:894	Moreover, the treated fiber was found suitable for light-weight applications since physical analysis acknowledges that the density of the fiber augmented to 1424 kg/m3after surface treatment that reduces total weight percentage.
34271053	2	45	theme	constituents	332:343	arg1	investigation					306:318	The investigation	302:318	The investigation of chemical constituents of optimally alkalized SISF	302:371	The investigation of chemical constituents of optimally alkalized SISF revealed that ideal increment of cellulose content (68.69 wt%) and desired modification of other chemical components was accomplished through 60 min immersion period.
34271053	2	46	theme	desired	440:446	arg1	modification					448:459	desired modification	440:459	desired modification of other chemical components	440:488	The investigation of chemical constituents of optimally alkalized SISF revealed that ideal increment of cellulose content (68.69 wt%) and desired modification of other chemical components was accomplished through 60 min immersion period.
34271053	0	47	from	stem	108:111	arg1	fibers					72:77	natural fibers	64:77	natural fibers from Symphirema involucratum stem	64:111	Comprehensive characterization of raw and alkali (NaOH) treated natural fibers from Symphirema involucratum stem.
34271053	2	48	theme	chemical	323:330	arg1	constituents					332:343	chemical constituents	323:343	chemical constituents of optimally alkalized SISF	323:371	The investigation of chemical constituents of optimally alkalized SISF revealed that ideal increment of cellulose content (68.69 wt%) and desired modification of other chemical components was accomplished through 60 min immersion period.
34271053	6	49	theme	composite	1245:1253	arg1	structures					1255:1264	lightweight composite structures	1233:1264	lightweight composite structures	1233:1264	These findings validated that the optimally surface-modified SISF is a suitable material for lightweight composite structures, for the time being.
34271053	1	50	theme	present	118:124	arg1	study					126:130	The present study	114:130	The present study	114:130	The present study investigated the effect of alkali treatment on the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF).
34271053	1	51	theme	tensile	216:222	arg1	enhancement					183:193	the enhancement	179:193	the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF)	179:299	The present study investigated the effect of alkali treatment on the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF).
34271053	3	52	theme	diffraction	646:656	arg1	analysis					658:665	X-ray diffraction analysis	640:665	X-ray diffraction analysis	640:665	An increase in the crystallinity index to 33.33% and small crystallite size to 3.21 nm was noted by X-ray diffraction analysis.
34271053	6	53	theme	lightweight	1233:1243	arg1	structures					1255:1264	lightweight composite structures	1233:1264	lightweight composite structures	1233:1264	These findings validated that the optimally surface-modified SISF is a suitable material for lightweight composite structures, for the time being.
34271053	5	54	theme	good	1065:1068	arg1	properties					1093:1102	good mechanical and thermal properties	1065:1102	good mechanical and thermal properties required for composite preparation	1065:1137	The enhancements in tensile strength (471.2 ± 19.8 MPa), tensile modulus (5.82 ± 0.77 GPa) and thermal stability (371 °C) were noted that ensures the treated fiber has good mechanical and thermal properties required for composite preparation.
34271053	3	55	theme	crystallite	599:609	arg1	size					611:614	small crystallite size	593:614	small crystallite size	593:614	An increase in the crystallinity index to 33.33% and small crystallite size to 3.21 nm was noted by X-ray diffraction analysis.
34271053	2	56	theme	SISF	368:371	arg1	constituents					332:343	chemical constituents	323:343	chemical constituents of optimally alkalized SISF	323:371	The investigation of chemical constituents of optimally alkalized SISF revealed that ideal increment of cellulose content (68.69 wt%) and desired modification of other chemical components was accomplished through 60 min immersion period.
34271053	2	57	theme	ideal	387:391	arg1	increment					393:401	ideal increment	387:401	ideal increment of cellulose content (68.69 wt%)	387:434	The investigation of chemical constituents of optimally alkalized SISF revealed that ideal increment of cellulose content (68.69 wt%) and desired modification of other chemical components was accomplished through 60 min immersion period.
34271053	1	58	theme	thermal	225:231	arg1	enhancement					183:193	the enhancement	179:193	the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF)	179:299	The present study investigated the effect of alkali treatment on the enhancement of Physico-chemical, tensile, thermal and surface properties of Symphirema involucratum stem fiber (SISF).
34271053	2	59	theme	alkalized	358:366	arg1	SISF					368:371	optimally alkalized SISF	348:371	optimally alkalized SISF	348:371	The investigation of chemical constituents of optimally alkalized SISF revealed that ideal increment of cellulose content (68.69 wt%) and desired modification of other chemical components was accomplished through 60 min immersion period.
33483077	0	0	theme	agricultural	76:87	arg1	cellulose					95:103	agricultural straw cellulose	76:103	agricultural straw cellulose	76:103	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.
33483077	2	1	theme	agricultural	412:423	arg1	straw					425:429	agricultural straw	412:429	agricultural straw (Zea mays)	412:440	Three representatively native fibers from softwood (Pinus kesiya), hardwood (Eucalyptus globulus), and agricultural straw (Zea mays) were selected to verify the effect of the method.
33483077	2	1	theme	agricultural	412:423	arg1	mays					436:439	Zea mays	432:439	Zea mays	432:439	Three representatively native fibers from softwood (Pinus kesiya), hardwood (Eucalyptus globulus), and agricultural straw (Zea mays) were selected to verify the effect of the method.
33483077	5	2	from	nature	968:973	arg1	nano-structures					947:961	nano-structures	947:961	nano-structures	947:961	The welding technique using inorganic metal salt hydrate provides a promising and convenient route to obtain firm sheet-materials with micro- or nano-structures from nature fibers.
33483077	0	3	from	preparation	7:17	arg1	cellulose					95:103	agricultural straw cellulose	76:103	agricultural straw cellulose	76:103	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.
33483077	0	3	from	preparation	7:17	arg1	hardwood					62:69	hardwood	62:69	hardwood	62:69	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.
33483077	0	3	from	preparation	7:17	arg1	softwood					52:59	softwood	52:59	softwood	52:59	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.
33483077	4	4	theme	thermostable	759:770	arg1	features					772:779	more robust and thermostable features	743:779	more robust and thermostable features	743:779	Cellulose sheets from the pine exhibited excellent mechanical properties (σb 16.94 MPa) and thermal stability (Tmax 265 °C) after the welding process, while the corn stalk sheets displayed more robust and thermostable features than the eucalyptus.
33483077	2	5	from	straw	425:429	arg1	fibers					339:344	Three representatively native fibers	309:344	Three representatively native fibers from softwood (Pinus kesiya), hardwood (Eucalyptus globulus), and agricultural straw (Zea mays)	309:440	Three representatively native fibers from softwood (Pinus kesiya), hardwood (Eucalyptus globulus), and agricultural straw (Zea mays) were selected to verify the effect of the method.
33483077	1	6	theme	molten	288:293	arg1	solvent					300:306	inorganic molten salt solvent	278:306	inorganic molten salt solvent	278:306	In this study, we report a novel, facile, and green method that was used for creating a new all-cellulose composite (ACC) based on inorganic molten salt solvent.
33483077	5	7	theme	promising	870:878	arg1	route					895:899	a promising and convenient route	868:899	a promising and convenient route to obtain firm sheet-materials with micro- or nano-structures from nature fibers	868:980	The welding technique using inorganic metal salt hydrate provides a promising and convenient route to obtain firm sheet-materials with micro- or nano-structures from nature fibers.
33483077	0	8	theme	straw	89:93	arg1	cellulose					95:103	agricultural straw cellulose	76:103	agricultural straw cellulose	76:103	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.
33483077	1	9	theme	salt	295:298	arg1	solvent					300:306	inorganic molten salt solvent	278:306	inorganic molten salt solvent	278:306	In this study, we report a novel, facile, and green method that was used for creating a new all-cellulose composite (ACC) based on inorganic molten salt solvent.
33483077	0	10	from	softwood	52:59	arg1	preparation					7:17	Facile preparation	0:17	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.	0:145	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.
33483077	0	10	from	softwood	52:59	arg1	composites					36:45	all-cellulose composites	22:45	all-cellulose composites from softwood, hardwood, and agricultural straw cellulose	22:103	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.
33483077	5	11	theme	convenient	884:893	arg1	route					895:899	a promising and convenient route	868:899	a promising and convenient route to obtain firm sheet-materials with micro- or nano-structures from nature fibers	868:980	The welding technique using inorganic metal salt hydrate provides a promising and convenient route to obtain firm sheet-materials with micro- or nano-structures from nature fibers.
33483077	4	12	from	pine	580:583	arg1	sheets					564:569	Cellulose sheets	554:569	Cellulose sheets from the pine	554:583	Cellulose sheets from the pine exhibited excellent mechanical properties (σb 16.94 MPa) and thermal stability (Tmax 265 °C) after the welding process, while the corn stalk sheets displayed more robust and thermostable features than the eucalyptus.
33483077	2	13	theme	Zea	432:434	arg1	straw					425:429	agricultural straw	412:429	agricultural straw (Zea mays)	412:440	Three representatively native fibers from softwood (Pinus kesiya), hardwood (Eucalyptus globulus), and agricultural straw (Zea mays) were selected to verify the effect of the method.
33483077	2	13	theme	Zea	432:434	arg1	mays					436:439	Zea mays	432:439	Zea mays	432:439	Three representatively native fibers from softwood (Pinus kesiya), hardwood (Eucalyptus globulus), and agricultural straw (Zea mays) were selected to verify the effect of the method.
33483077	0	14	theme	Facile	0:5	arg1	preparation					7:17	Facile preparation	0:17	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.	0:145	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.
33483077	5	15	theme	salt	846:849	arg1	hydrate					851:857	inorganic metal salt hydrate	830:857	inorganic metal salt hydrate	830:857	The welding technique using inorganic metal salt hydrate provides a promising and convenient route to obtain firm sheet-materials with micro- or nano-structures from nature fibers.
33483077	2	16	theme	native	332:337	arg1	fibers					339:344	Three representatively native fibers	309:344	Three representatively native fibers from softwood (Pinus kesiya), hardwood (Eucalyptus globulus), and agricultural straw (Zea mays)	309:440	Three representatively native fibers from softwood (Pinus kesiya), hardwood (Eucalyptus globulus), and agricultural straw (Zea mays) were selected to verify the effect of the method.
33483077	1	17	theme	inorganic	278:286	arg1	solvent					300:306	inorganic molten salt solvent	278:306	inorganic molten salt solvent	278:306	In this study, we report a novel, facile, and green method that was used for creating a new all-cellulose composite (ACC) based on inorganic molten salt solvent.
33483077	2	18	dep	hardwood	376:383	arg1	globulus					397:404	Eucalyptus globulus	386:404	Eucalyptus globulus	386:404	Three representatively native fibers from softwood (Pinus kesiya), hardwood (Eucalyptus globulus), and agricultural straw (Zea mays) were selected to verify the effect of the method.
33483077	4	19	theme	thermal	646:652	arg1	Tmax					665:668	Tmax 265 °C	665:675	Tmax 265 °C	665:675	Cellulose sheets from the pine exhibited excellent mechanical properties (σb 16.94 MPa) and thermal stability (Tmax 265 °C) after the welding process, while the corn stalk sheets displayed more robust and thermostable features than the eucalyptus.
33483077	4	19	theme	thermal	646:652	arg1	stability					654:662	thermal stability	646:662	thermal stability (Tmax 265 °C)	646:676	Cellulose sheets from the pine exhibited excellent mechanical properties (σb 16.94 MPa) and thermal stability (Tmax 265 °C) after the welding process, while the corn stalk sheets displayed more robust and thermostable features than the eucalyptus.
33483077	2	20	theme	representatively	315:330	arg1	fibers					339:344	Three representatively native fibers	309:344	Three representatively native fibers from softwood (Pinus kesiya), hardwood (Eucalyptus globulus), and agricultural straw (Zea mays)	309:440	Three representatively native fibers from softwood (Pinus kesiya), hardwood (Eucalyptus globulus), and agricultural straw (Zea mays) were selected to verify the effect of the method.
33483077	0	21	theme	all-cellulose	22:34	arg1	composites					36:45	all-cellulose composites	22:45	all-cellulose composites from softwood, hardwood, and agricultural straw cellulose	22:103	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.
33483077	5	22	theme	nano-structures	947:961	arg1	fibers					975:980	micro- or nano-structures from nature fibers	937:980	micro- or nano-structures from nature fibers	937:980	The welding technique using inorganic metal salt hydrate provides a promising and convenient route to obtain firm sheet-materials with micro- or nano-structures from nature fibers.
33483077	4	23	theme	corn	715:718	arg1	sheets					726:731	the corn stalk sheets	711:731	the corn stalk sheets	711:731	Cellulose sheets from the pine exhibited excellent mechanical properties (σb 16.94 MPa) and thermal stability (Tmax 265 °C) after the welding process, while the corn stalk sheets displayed more robust and thermostable features than the eucalyptus.
33483077	1	24	theme	new	235:237	arg1	ACC					264:266	ACC	264:266	ACC	264:266	In this study, we report a novel, facile, and green method that was used for creating a new all-cellulose composite (ACC) based on inorganic molten salt solvent.
33483077	1	24	theme	new	235:237	arg1	composite					253:261	a new all-cellulose composite	233:261	a new all-cellulose composite (ACC) based on inorganic molten salt solvent	233:306	In this study, we report a novel, facile, and green method that was used for creating a new all-cellulose composite (ACC) based on inorganic molten salt solvent.
33483077	0	25	theme	simple	110:115	arg1	route					117:121	a simple route	108:121	a simple route of partial dissolution	108:144	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.
33483077	5	26	theme	welding	806:812	arg1	technique					814:822	The welding technique	802:822	The welding technique using inorganic metal salt hydrate	802:857	The welding technique using inorganic metal salt hydrate provides a promising and convenient route to obtain firm sheet-materials with micro- or nano-structures from nature fibers.
33483077	1	27	theme	novel	174:178	arg1	method					199:204	a novel, facile, and green method	172:204	a novel, facile, and green method that was used for creating a new all-cellulose composite (ACC) based on inorganic molten salt solvent	172:306	In this study, we report a novel, facile, and green method that was used for creating a new all-cellulose composite (ACC) based on inorganic molten salt solvent.
33483077	1	28	theme	all-cellulose	239:251	arg1	ACC					264:266	ACC	264:266	ACC	264:266	In this study, we report a novel, facile, and green method that was used for creating a new all-cellulose composite (ACC) based on inorganic molten salt solvent.
33483077	1	28	theme	all-cellulose	239:251	arg1	composite					253:261	a new all-cellulose composite	233:261	a new all-cellulose composite (ACC) based on inorganic molten salt solvent	233:306	In this study, we report a novel, facile, and green method that was used for creating a new all-cellulose composite (ACC) based on inorganic molten salt solvent.
33483077	0	29	theme	partial	126:132	arg1	dissolution					134:144	partial dissolution	126:144	partial dissolution	126:144	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.
33483077	1	30	used	used	215:218	arg2	method					199:204	a novel, facile, and green method	172:204	a novel, facile, and green method that was used for creating a new all-cellulose composite (ACC) based on inorganic molten salt solvent	172:306	In this study, we report a novel, facile, and green method that was used for creating a new all-cellulose composite (ACC) based on inorganic molten salt solvent.
33483077	3	31	theme	welded	496:501	arg1	sheets					503:508	The welded sheets	492:508	The welded sheets	492:508	The welded sheets were thoroughly characterized and compared.
33483077	0	32	theme	composites	36:45	arg1	preparation					7:17	Facile preparation	0:17	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.	0:145	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.
33483077	5	33	theme	micro-	937:942	arg1	fibers					975:980	micro- or nano-structures from nature fibers	937:980	micro- or nano-structures from nature fibers	937:980	The welding technique using inorganic metal salt hydrate provides a promising and convenient route to obtain firm sheet-materials with micro- or nano-structures from nature fibers.
33483077	1	34	theme	facile	181:186	arg1	method					199:204	a novel, facile, and green method	172:204	a novel, facile, and green method that was used for creating a new all-cellulose composite (ACC) based on inorganic molten salt solvent	172:306	In this study, we report a novel, facile, and green method that was used for creating a new all-cellulose composite (ACC) based on inorganic molten salt solvent.
33483077	2	35	theme	method	484:489	arg1	effect					470:475	the effect	466:475	the effect of the method	466:489	Three representatively native fibers from softwood (Pinus kesiya), hardwood (Eucalyptus globulus), and agricultural straw (Zea mays) were selected to verify the effect of the method.
33483077	0	36	from	hardwood	62:69	arg1	preparation					7:17	Facile preparation	0:17	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.	0:145	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.
33483077	0	36	from	hardwood	62:69	arg1	composites					36:45	all-cellulose composites	22:45	all-cellulose composites from softwood, hardwood, and agricultural straw cellulose	22:103	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.
33483077	4	37	theme	robust	748:753	arg1	features					772:779	more robust and thermostable features	743:779	more robust and thermostable features	743:779	Cellulose sheets from the pine exhibited excellent mechanical properties (σb 16.94 MPa) and thermal stability (Tmax 265 °C) after the welding process, while the corn stalk sheets displayed more robust and thermostable features than the eucalyptus.
33483077	2	38	from	hardwood	376:383	arg1	fibers					339:344	Three representatively native fibers	309:344	Three representatively native fibers from softwood (Pinus kesiya), hardwood (Eucalyptus globulus), and agricultural straw (Zea mays)	309:440	Three representatively native fibers from softwood (Pinus kesiya), hardwood (Eucalyptus globulus), and agricultural straw (Zea mays) were selected to verify the effect of the method.
33483077	0	39	theme	dissolution	134:144	arg1	route					117:121	a simple route	108:121	a simple route of partial dissolution	108:144	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.
33483077	4	40	theme	stalk	720:724	arg1	sheets					726:731	the corn stalk sheets	711:731	the corn stalk sheets	711:731	Cellulose sheets from the pine exhibited excellent mechanical properties (σb 16.94 MPa) and thermal stability (Tmax 265 °C) after the welding process, while the corn stalk sheets displayed more robust and thermostable features than the eucalyptus.
33483077	5	41	theme	inorganic	830:838	arg1	hydrate					851:857	inorganic metal salt hydrate	830:857	inorganic metal salt hydrate	830:857	The welding technique using inorganic metal salt hydrate provides a promising and convenient route to obtain firm sheet-materials with micro- or nano-structures from nature fibers.
33483077	0	42	from	cellulose	95:103	arg1	preparation					7:17	Facile preparation	0:17	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.	0:145	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.
33483077	0	42	from	cellulose	95:103	arg1	composites					36:45	all-cellulose composites	22:45	all-cellulose composites from softwood, hardwood, and agricultural straw cellulose	22:103	Facile preparation of all-cellulose composites from softwood, hardwood, and agricultural straw cellulose by a simple route of partial dissolution.
33483077	4	43	theme	welding	688:694	arg1	process					696:702	the welding process	684:702	the welding process	684:702	Cellulose sheets from the pine exhibited excellent mechanical properties (σb 16.94 MPa) and thermal stability (Tmax 265 °C) after the welding process, while the corn stalk sheets displayed more robust and thermostable features than the eucalyptus.
33483077	5	44	theme	metal	840:844	arg1	hydrate					851:857	inorganic metal salt hydrate	830:857	inorganic metal salt hydrate	830:857	The welding technique using inorganic metal salt hydrate provides a promising and convenient route to obtain firm sheet-materials with micro- or nano-structures from nature fibers.
33483077	4	45	theme	Cellulose	554:562	arg1	sheets					564:569	Cellulose sheets	554:569	Cellulose sheets from the pine	554:583	Cellulose sheets from the pine exhibited excellent mechanical properties (σb 16.94 MPa) and thermal stability (Tmax 265 °C) after the welding process, while the corn stalk sheets displayed more robust and thermostable features than the eucalyptus.
33483077	5	46	theme	firm	911:914	arg1	sheet-materials					916:930	firm sheet-materials	911:930	firm sheet-materials with micro- or nano-structures from nature fibers	911:980	The welding technique using inorganic metal salt hydrate provides a promising and convenient route to obtain firm sheet-materials with micro- or nano-structures from nature fibers.
33483077	2	47	from	softwood	351:358	arg1	fibers					339:344	Three representatively native fibers	309:344	Three representatively native fibers from softwood (Pinus kesiya), hardwood (Eucalyptus globulus), and agricultural straw (Zea mays)	309:440	Three representatively native fibers from softwood (Pinus kesiya), hardwood (Eucalyptus globulus), and agricultural straw (Zea mays) were selected to verify the effect of the method.
33483077	4	48	theme	mechanical	605:614	arg1	properties					616:625	excellent mechanical properties	595:625	excellent mechanical properties (σb 16.94 MPa)	595:640	Cellulose sheets from the pine exhibited excellent mechanical properties (σb 16.94 MPa) and thermal stability (Tmax 265 °C) after the welding process, while the corn stalk sheets displayed more robust and thermostable features than the eucalyptus.
33483077	4	48	theme	mechanical	605:614	arg1	16.94 MPa					631:639	σb 16.94 MPa	628:639	σb 16.94 MPa	628:639	Cellulose sheets from the pine exhibited excellent mechanical properties (σb 16.94 MPa) and thermal stability (Tmax 265 °C) after the welding process, while the corn stalk sheets displayed more robust and thermostable features than the eucalyptus.
33483077	1	49	theme	green	193:197	arg1	method					199:204	a novel, facile, and green method	172:204	a novel, facile, and green method that was used for creating a new all-cellulose composite (ACC) based on inorganic molten salt solvent	172:306	In this study, we report a novel, facile, and green method that was used for creating a new all-cellulose composite (ACC) based on inorganic molten salt solvent.
33483077	5	50	with	sheet-materials	916:930	arg1	fibers					975:980	micro- or nano-structures from nature fibers	937:980	micro- or nano-structures from nature fibers	937:980	The welding technique using inorganic metal salt hydrate provides a promising and convenient route to obtain firm sheet-materials with micro- or nano-structures from nature fibers.
33483077	4	51	theme	excellent	595:603	arg1	properties					616:625	excellent mechanical properties	595:625	excellent mechanical properties (σb 16.94 MPa)	595:640	Cellulose sheets from the pine exhibited excellent mechanical properties (σb 16.94 MPa) and thermal stability (Tmax 265 °C) after the welding process, while the corn stalk sheets displayed more robust and thermostable features than the eucalyptus.
33483077	4	51	theme	excellent	595:603	arg1	16.94 MPa					631:639	σb 16.94 MPa	628:639	σb 16.94 MPa	628:639	Cellulose sheets from the pine exhibited excellent mechanical properties (σb 16.94 MPa) and thermal stability (Tmax 265 °C) after the welding process, while the corn stalk sheets displayed more robust and thermostable features than the eucalyptus.
34968551	0	0	theme	diabetic	73:80	arg1	mice					82:85	diabetic mice	73:85	diabetic mice	73:85	The effect of high-amylose resistant starch on the glycogen structure of diabetic mice.
34968551	8	1	theme	liver	1091:1095	arg1	glycogen					1097:1104	liver glycogen	1091:1104	liver glycogen	1091:1104	Molecular size distributions and chain-length distributions of liver glycogen from both groups were characterized to test glycogen fragility before and after DMSO treatment.
34968551	5	2	from	effects	787:793	arg1	response					807:814	glycemic response	798:814	glycemic response	798:814	High-amylose resistant starch (RS) can escape digestion in the small intestine and ferment in the large intestine, which elicits positive effects on glycemic response and type 2 diabetes.
34968551	5	2	from	effects	787:793	arg1	diabetes					827:834	type 2 diabetes	820:834	type 2 diabetes	820:834	High-amylose resistant starch (RS) can escape digestion in the small intestine and ferment in the large intestine, which elicits positive effects on glycemic response and type 2 diabetes.
34968551	5	3	theme	type	820:823	arg1	diabetes					827:834	type 2 diabetes	820:834	type 2 diabetes	820:834	High-amylose resistant starch (RS) can escape digestion in the small intestine and ferment in the large intestine, which elicits positive effects on glycemic response and type 2 diabetes.
34968551	8	4	theme	size	1038:1041	arg1	distributions					1043:1055	Molecular size distributions	1028:1055	Molecular size distributions	1028:1055	Molecular size distributions and chain-length distributions of liver glycogen from both groups were characterized to test glycogen fragility before and after DMSO treatment.
34968551	4	5	theme	enzymatic	500:508	arg1	degradation					510:520	such a rapid enzymatic degradation	487:520	such a rapid enzymatic degradation of these smaller particles into glucose	487:560	If this occurs in vivo, such a rapid enzymatic degradation of these smaller particles into glucose could exacerbate the poor blood-glucose control that is characteristic of the disease.
34968551	3	6	theme	mouse	283:287	arg1	models					289:294	mouse models	283:294	mouse models of diabetes	283:306	In mouse models of diabetes, these liver glycogen particles are molecularly fragile, breaking up into smaller particles in the presence of solvents such as dimethyl sulfoxide (DMSO).
34968551	0	7	from	effect	4:9	arg1	structure					60:68	the glycogen structure	47:68	the glycogen structure of diabetic mice	47:85	The effect of high-amylose resistant starch on the glycogen structure of diabetic mice.
34968551	10	8	theme	significant	1391:1401	arg1	effect					1403:1408	no significant effect	1388:1408	no significant effect	1388:1408	The diets had no significant effect on the glycogen chain-length distributions.
34968551	1	9	theme	many	144:147	arg1	tissues					149:155	many tissues	144:155	many tissues	144:155	Glycogen is a complex branched glucose polymer found in many tissues and acts as a blood-glucose buffer.
34968551	4	10	theme	rapid	494:498	arg1	degradation					510:520	such a rapid enzymatic degradation	487:520	such a rapid enzymatic degradation of these smaller particles into glucose	487:560	If this occurs in vivo, such a rapid enzymatic degradation of these smaller particles into glucose could exacerbate the poor blood-glucose control that is characteristic of the disease.
34968551	4	11	theme	particles	539:547	arg1	degradation					510:520	such a rapid enzymatic degradation	487:520	such a rapid enzymatic degradation of these smaller particles into glucose	487:560	If this occurs in vivo, such a rapid enzymatic degradation of these smaller particles into glucose could exacerbate the poor blood-glucose control that is characteristic of the disease.
34968551	2	12	theme	larger	250:255	arg1	particles					269:277	larger composite α particles	250:277	larger composite α particles	250:277	In the liver, smaller β glycogen particles can bind into larger composite α particles.
34968551	3	13	theme	liver	315:319	arg1	particles					330:338	these liver glycogen particles	309:338	these liver glycogen particles	309:338	In mouse models of diabetes, these liver glycogen particles are molecularly fragile, breaking up into smaller particles in the presence of solvents such as dimethyl sulfoxide (DMSO).
34968551	1	14	located	found	135:139	arg1	tissues					149:155	many tissues	144:155	many tissues	144:155	Glycogen is a complex branched glucose polymer found in many tissues and acts as a blood-glucose buffer.
34968551	1	14	located	found	135:139	arg2	Glycogen					88:95	Glycogen	88:95	Glycogen	88:95	Glycogen is a complex branched glucose polymer found in many tissues and acts as a blood-glucose buffer.
34968551	1	14	located	found	135:139	arg2	polymer					127:133	a complex branched glucose polymer	100:133	a complex branched glucose polymer found in many tissues	100:155	Glycogen is a complex branched glucose polymer found in many tissues and acts as a blood-glucose buffer.
34968551	5	15	theme	positive	778:785	arg1	effects					787:793	positive effects	778:793	positive effects on glycemic response and type 2 diabetes	778:834	High-amylose resistant starch (RS) can escape digestion in the small intestine and ferment in the large intestine, which elicits positive effects on glycemic response and type 2 diabetes.
34968551	3	16	theme	diabetes	299:306	arg1	models					289:294	mouse models	283:294	mouse models of diabetes	283:306	In mouse models of diabetes, these liver glycogen particles are molecularly fragile, breaking up into smaller particles in the presence of solvents such as dimethyl sulfoxide (DMSO).
34968551	10	17	theme	chain-length	1426:1437	arg1	distributions					1439:1451	the glycogen chain-length distributions	1413:1451	the glycogen chain-length distributions	1413:1451	The diets had no significant effect on the glycogen chain-length distributions.
34968551	3	18	theme	solvents	419:426	arg1	presence					407:414	the presence	403:414	the presence of solvents such as dimethyl sulfoxide (DMSO)	403:460	In mouse models of diabetes, these liver glycogen particles are molecularly fragile, breaking up into smaller particles in the presence of solvents such as dimethyl sulfoxide (DMSO).
34968551	4	19	theme	blood-glucose	588:600	arg1	control					602:608	the poor blood-glucose control	579:608	the poor blood-glucose control that is characteristic of the disease	579:646	If this occurs in vivo, such a rapid enzymatic degradation of these smaller particles into glucose could exacerbate the poor blood-glucose control that is characteristic of the disease.
34968551	4	19	theme	blood-glucose	588:600	arg1	characteristic					618:631	characteristic	618:631	characteristic of the disease	618:646	If this occurs in vivo, such a rapid enzymatic degradation of these smaller particles into glucose could exacerbate the poor blood-glucose control that is characteristic of the disease.
34968551	6	20	theme	glycogen	907:914	arg1	fragility					916:924	diabetes-related liver glycogen fragility	884:924	diabetes-related liver glycogen fragility	884:924	Here we postulate that RS would help attenuate diabetes-related liver glycogen fragility.
34968551	3	21	theme	breaking	365:372	arg1	up					374:375	breaking up	365:375	breaking up into smaller particles in the presence of solvents such as dimethyl sulfoxide (DMSO)	365:460	In mouse models of diabetes, these liver glycogen particles are molecularly fragile, breaking up into smaller particles in the presence of solvents such as dimethyl sulfoxide (DMSO).
34968551	9	22	with	Consistent	1202:1211	arg1	hypothesis					1222:1231	the hypothesis that high blood glucose is associated with glycogen fragility	1218:1293	the hypothesis that high blood glucose is associated with glycogen fragility	1218:1293	Consistent with the hypothesis that high blood glucose is associated with glycogen fragility, a high-amylose RS diet prevented the fragility of liver-glycogen α particles.
34968551	4	23	theme	poor	583:586	arg1	control					602:608	the poor blood-glucose control	579:608	the poor blood-glucose control that is characteristic of the disease	579:646	If this occurs in vivo, such a rapid enzymatic degradation of these smaller particles into glucose could exacerbate the poor blood-glucose control that is characteristic of the disease.
34968551	4	23	theme	poor	583:586	arg1	characteristic					618:631	characteristic	618:631	characteristic of the disease	618:646	If this occurs in vivo, such a rapid enzymatic degradation of these smaller particles into glucose could exacerbate the poor blood-glucose control that is characteristic of the disease.
34968551	9	24	theme	high-amylose	1298:1309	arg1	diet					1314:1317	a high-amylose RS diet	1296:1317	a high-amylose RS diet	1296:1317	Consistent with the hypothesis that high blood glucose is associated with glycogen fragility, a high-amylose RS diet prevented the fragility of liver-glycogen α particles.
34968551	8	25	theme	Molecular	1028:1036	arg1	distributions					1043:1055	Molecular size distributions	1028:1055	Molecular size distributions	1028:1055	Molecular size distributions and chain-length distributions of liver glycogen from both groups were characterized to test glycogen fragility before and after DMSO treatment.
34968551	9	26	theme	blood	1243:1247	arg1	glucose					1249:1255	high blood glucose	1238:1255	high blood glucose	1238:1255	Consistent with the hypothesis that high blood glucose is associated with glycogen fragility, a high-amylose RS diet prevented the fragility of liver-glycogen α particles.
34968551	6	27	theme	liver	901:905	arg1	fragility					916:924	diabetes-related liver glycogen fragility	884:924	diabetes-related liver glycogen fragility	884:924	Here we postulate that RS would help attenuate diabetes-related liver glycogen fragility.
34968551	7	28	theme	non-diabetic	1009:1020	arg1	mice					1022:1025	diabetic and non-diabetic mice	996:1025	diabetic and non-diabetic mice	996:1025	Normal maize starch and two types of high-amylose starch were fed to diabetic and non-diabetic mice.
34968551	8	29	from	groups	1116:1121	arg1	distributions					1043:1055	Molecular size distributions	1028:1055	Molecular size distributions	1028:1055	Molecular size distributions and chain-length distributions of liver glycogen from both groups were characterized to test glycogen fragility before and after DMSO treatment.
34968551	8	29	from	groups	1116:1121	arg1	distributions					1074:1086	chain-length distributions	1061:1086	chain-length distributions	1061:1086	Molecular size distributions and chain-length distributions of liver glycogen from both groups were characterized to test glycogen fragility before and after DMSO treatment.
34968551	3	30	theme	smaller	382:388	arg1	particles					390:398	smaller particles	382:398	smaller particles in the presence of solvents such as dimethyl sulfoxide (DMSO)	382:460	In mouse models of diabetes, these liver glycogen particles are molecularly fragile, breaking up into smaller particles in the presence of solvents such as dimethyl sulfoxide (DMSO).
34968551	0	31	theme	high-amylose	14:25	arg1	starch					37:42	high-amylose resistant starch	14:42	high-amylose resistant starch	14:42	The effect of high-amylose resistant starch on the glycogen structure of diabetic mice.
34968551	6	32	theme	diabetes-related	884:899	arg1	fragility					916:924	diabetes-related liver glycogen fragility	884:924	diabetes-related liver glycogen fragility	884:924	Here we postulate that RS would help attenuate diabetes-related liver glycogen fragility.
34968551	2	33	theme	α	267:267	arg1	particles					269:277	larger composite α particles	250:277	larger composite α particles	250:277	In the liver, smaller β glycogen particles can bind into larger composite α particles.
34968551	5	34	theme	large	747:751	arg1	intestine					753:761	the large intestine	743:761	the large intestine	743:761	High-amylose resistant starch (RS) can escape digestion in the small intestine and ferment in the large intestine, which elicits positive effects on glycemic response and type 2 diabetes.
34968551	7	35	theme	diabetic	996:1003	arg1	mice					1022:1025	diabetic and non-diabetic mice	996:1025	diabetic and non-diabetic mice	996:1025	Normal maize starch and two types of high-amylose starch were fed to diabetic and non-diabetic mice.
34968551	2	36	theme	composite	257:265	arg1	particles					269:277	larger composite α particles	250:277	larger composite α particles	250:277	In the liver, smaller β glycogen particles can bind into larger composite α particles.
34968551	1	37	theme	blood-glucose	171:183	arg1	Glycogen					88:95	Glycogen	88:95	Glycogen	88:95	Glycogen is a complex branched glucose polymer found in many tissues and acts as a blood-glucose buffer.
34968551	1	37	theme	blood-glucose	171:183	arg1	buffer					185:190	a blood-glucose buffer	169:190	a blood-glucose buffer	169:190	Glycogen is a complex branched glucose polymer found in many tissues and acts as a blood-glucose buffer.
34968551	0	38	theme	starch	37:42	arg1	effect					4:9	The effect	0:9	The effect of high-amylose resistant starch on the glycogen structure of diabetic mice	0:85	The effect of high-amylose resistant starch on the glycogen structure of diabetic mice.
34968551	4	39	theme	disease	640:646	arg1	control					602:608	the poor blood-glucose control	579:608	the poor blood-glucose control that is characteristic of the disease	579:646	If this occurs in vivo, such a rapid enzymatic degradation of these smaller particles into glucose could exacerbate the poor blood-glucose control that is characteristic of the disease.
34968551	4	39	theme	disease	640:646	arg1	characteristic					618:631	characteristic	618:631	characteristic of the disease	618:646	If this occurs in vivo, such a rapid enzymatic degradation of these smaller particles into glucose could exacerbate the poor blood-glucose control that is characteristic of the disease.
34968551	5	40	theme	High-amylose	649:660	arg1	RS					680:681	RS	680:681	RS	680:681	High-amylose resistant starch (RS) can escape digestion in the small intestine and ferment in the large intestine, which elicits positive effects on glycemic response and type 2 diabetes.
34968551	5	40	theme	High-amylose	649:660	arg1	starch					672:677	High-amylose resistant starch	649:677	High-amylose resistant starch (RS)	649:682	High-amylose resistant starch (RS) can escape digestion in the small intestine and ferment in the large intestine, which elicits positive effects on glycemic response and type 2 diabetes.
34968551	9	41	theme	high	1238:1241	arg1	glucose					1249:1255	high blood glucose	1238:1255	high blood glucose	1238:1255	Consistent with the hypothesis that high blood glucose is associated with glycogen fragility, a high-amylose RS diet prevented the fragility of liver-glycogen α particles.
34968551	5	42	theme	glycemic	798:805	arg1	response					807:814	glycemic response	798:814	glycemic response	798:814	High-amylose resistant starch (RS) can escape digestion in the small intestine and ferment in the large intestine, which elicits positive effects on glycemic response and type 2 diabetes.
34968551	0	43	theme	resistant	27:35	arg1	starch					37:42	high-amylose resistant starch	14:42	high-amylose resistant starch	14:42	The effect of high-amylose resistant starch on the glycogen structure of diabetic mice.
34968551	9	44	theme	RS	1311:1312	arg1	diet					1314:1317	a high-amylose RS diet	1296:1317	a high-amylose RS diet	1296:1317	Consistent with the hypothesis that high blood glucose is associated with glycogen fragility, a high-amylose RS diet prevented the fragility of liver-glycogen α particles.
34968551	5	45	theme	resistant	662:670	arg1	RS					680:681	RS	680:681	RS	680:681	High-amylose resistant starch (RS) can escape digestion in the small intestine and ferment in the large intestine, which elicits positive effects on glycemic response and type 2 diabetes.
34968551	5	45	theme	resistant	662:670	arg1	starch					672:677	High-amylose resistant starch	649:677	High-amylose resistant starch (RS)	649:682	High-amylose resistant starch (RS) can escape digestion in the small intestine and ferment in the large intestine, which elicits positive effects on glycemic response and type 2 diabetes.
34968551	3	46	theme	glycogen	321:328	arg1	particles					330:338	these liver glycogen particles	309:338	these liver glycogen particles	309:338	In mouse models of diabetes, these liver glycogen particles are molecularly fragile, breaking up into smaller particles in the presence of solvents such as dimethyl sulfoxide (DMSO).
34968551	9	47	theme	particles	1363:1371	arg1	fragility					1333:1341	the fragility	1329:1341	the fragility of liver-glycogen α particles	1329:1371	Consistent with the hypothesis that high blood glucose is associated with glycogen fragility, a high-amylose RS diet prevented the fragility of liver-glycogen α particles.
34968551	7	48	theme	high-amylose	964:975	arg1	starch					977:982	high-amylose starch	964:982	high-amylose starch	964:982	Normal maize starch and two types of high-amylose starch were fed to diabetic and non-diabetic mice.
34968551	8	49	theme	chain-length	1061:1072	arg1	distributions					1074:1086	chain-length distributions	1061:1086	chain-length distributions	1061:1086	Molecular size distributions and chain-length distributions of liver glycogen from both groups were characterized to test glycogen fragility before and after DMSO treatment.
34968551	3	50	theme	dimethyl	436:443	arg1	DMSO					456:459	DMSO	456:459	DMSO	456:459	In mouse models of diabetes, these liver glycogen particles are molecularly fragile, breaking up into smaller particles in the presence of solvents such as dimethyl sulfoxide (DMSO).
34968551	3	50	theme	dimethyl	436:443	arg1	sulfoxide					445:453	dimethyl sulfoxide	436:453	dimethyl sulfoxide (DMSO)	436:460	In mouse models of diabetes, these liver glycogen particles are molecularly fragile, breaking up into smaller particles in the presence of solvents such as dimethyl sulfoxide (DMSO).
34968551	7	51	theme	starch	977:982	arg1	starch					940:945	Normal maize starch	927:945	Normal maize starch	927:945	Normal maize starch and two types of high-amylose starch were fed to diabetic and non-diabetic mice.
34968551	7	51	theme	starch	977:982	arg1	types					955:959	two types	951:959	two types of high-amylose starch	951:982	Normal maize starch and two types of high-amylose starch were fed to diabetic and non-diabetic mice.
34968551	5	52	from	digestion	695:703	arg1	intestine					718:726	the small intestine	708:726	the small intestine	708:726	High-amylose resistant starch (RS) can escape digestion in the small intestine and ferment in the large intestine, which elicits positive effects on glycemic response and type 2 diabetes.
34968551	10	53	theme	glycogen	1417:1424	arg1	distributions					1439:1451	the glycogen chain-length distributions	1413:1451	the glycogen chain-length distributions	1413:1451	The diets had no significant effect on the glycogen chain-length distributions.
34968551	7	54	theme	maize	934:938	arg1	starch					940:945	Normal maize starch	927:945	Normal maize starch	927:945	Normal maize starch and two types of high-amylose starch were fed to diabetic and non-diabetic mice.
34968551	1	55	theme	complex	102:108	arg1	Glycogen					88:95	Glycogen	88:95	Glycogen	88:95	Glycogen is a complex branched glucose polymer found in many tissues and acts as a blood-glucose buffer.
34968551	1	55	theme	complex	102:108	arg1	polymer					127:133	a complex branched glucose polymer	100:133	a complex branched glucose polymer found in many tissues	100:155	Glycogen is a complex branched glucose polymer found in many tissues and acts as a blood-glucose buffer.
34968551	8	56	theme	glycogen	1097:1104	arg1	distributions					1043:1055	Molecular size distributions	1028:1055	Molecular size distributions	1028:1055	Molecular size distributions and chain-length distributions of liver glycogen from both groups were characterized to test glycogen fragility before and after DMSO treatment.
34968551	8	56	theme	glycogen	1097:1104	arg1	distributions					1074:1086	chain-length distributions	1061:1086	chain-length distributions	1061:1086	Molecular size distributions and chain-length distributions of liver glycogen from both groups were characterized to test glycogen fragility before and after DMSO treatment.
34968551	5	57	theme	small	712:716	arg1	intestine					718:726	the small intestine	708:726	the small intestine	708:726	High-amylose resistant starch (RS) can escape digestion in the small intestine and ferment in the large intestine, which elicits positive effects on glycemic response and type 2 diabetes.
34968551	1	58	theme	branched	110:117	arg1	Glycogen					88:95	Glycogen	88:95	Glycogen	88:95	Glycogen is a complex branched glucose polymer found in many tissues and acts as a blood-glucose buffer.
34968551	1	58	theme	branched	110:117	arg1	polymer					127:133	a complex branched glucose polymer	100:133	a complex branched glucose polymer found in many tissues	100:155	Glycogen is a complex branched glucose polymer found in many tissues and acts as a blood-glucose buffer.
34968551	4	59	theme	smaller	531:537	arg1	particles					539:547	these smaller particles	525:547	these smaller particles	525:547	If this occurs in vivo, such a rapid enzymatic degradation of these smaller particles into glucose could exacerbate the poor blood-glucose control that is characteristic of the disease.
34968551	10	60	contain	had	1384:1386	arg2	effect					1403:1408	no significant effect	1388:1408	no significant effect	1388:1408	The diets had no significant effect on the glycogen chain-length distributions.
34968551	10	60	contain	had	1384:1386	arg1	diets					1378:1382	The diets	1374:1382	The diets	1374:1382	The diets had no significant effect on the glycogen chain-length distributions.
34968551	3	61	from	models	289:294	arg1	fragile					356:362	fragile	356:362	fragile	356:362	In mouse models of diabetes, these liver glycogen particles are molecularly fragile, breaking up into smaller particles in the presence of solvents such as dimethyl sulfoxide (DMSO).
34968551	2	62	theme	glycogen	217:224	arg1	particles					226:234	smaller β glycogen particles	207:234	smaller β glycogen particles	207:234	In the liver, smaller β glycogen particles can bind into larger composite α particles.
34968551	1	63	theme	glucose	119:125	arg1	Glycogen					88:95	Glycogen	88:95	Glycogen	88:95	Glycogen is a complex branched glucose polymer found in many tissues and acts as a blood-glucose buffer.
34968551	1	63	theme	glucose	119:125	arg1	polymer					127:133	a complex branched glucose polymer	100:133	a complex branched glucose polymer found in many tissues	100:155	Glycogen is a complex branched glucose polymer found in many tissues and acts as a blood-glucose buffer.
34968551	8	64	theme	glycogen	1150:1157	arg1	fragility					1159:1167	glycogen fragility	1150:1167	glycogen fragility	1150:1167	Molecular size distributions and chain-length distributions of liver glycogen from both groups were characterized to test glycogen fragility before and after DMSO treatment.
34968551	9	65	theme	glycogen	1276:1283	arg1	fragility					1285:1293	glycogen fragility	1276:1293	glycogen fragility	1276:1293	Consistent with the hypothesis that high blood glucose is associated with glycogen fragility, a high-amylose RS diet prevented the fragility of liver-glycogen α particles.
34968551	2	66	theme	β	215:215	arg1	particles					226:234	smaller β glycogen particles	207:234	smaller β glycogen particles	207:234	In the liver, smaller β glycogen particles can bind into larger composite α particles.
34968551	7	67	theme	Normal	927:932	arg1	starch					940:945	Normal maize starch	927:945	Normal maize starch	927:945	Normal maize starch and two types of high-amylose starch were fed to diabetic and non-diabetic mice.
34968551	9	68	theme	liver-glycogen	1346:1359	arg1	particles					1363:1371	liver-glycogen α particles	1346:1371	liver-glycogen α particles	1346:1371	Consistent with the hypothesis that high blood glucose is associated with glycogen fragility, a high-amylose RS diet prevented the fragility of liver-glycogen α particles.
34968551	0	69	theme	glycogen	51:58	arg1	structure					60:68	the glycogen structure	47:68	the glycogen structure of diabetic mice	47:85	The effect of high-amylose resistant starch on the glycogen structure of diabetic mice.
34968551	3	70	from	fragile	356:362	arg1	models					289:294	mouse models	283:294	mouse models of diabetes	283:306	In mouse models of diabetes, these liver glycogen particles are molecularly fragile, breaking up into smaller particles in the presence of solvents such as dimethyl sulfoxide (DMSO).
34968551	2	71	theme	smaller	207:213	arg1	particles					226:234	smaller β glycogen particles	207:234	smaller β glycogen particles	207:234	In the liver, smaller β glycogen particles can bind into larger composite α particles.
34968551	3	72	from	particles	390:398	arg1	presence					407:414	the presence	403:414	the presence of solvents such as dimethyl sulfoxide (DMSO)	403:460	In mouse models of diabetes, these liver glycogen particles are molecularly fragile, breaking up into smaller particles in the presence of solvents such as dimethyl sulfoxide (DMSO).
34968551	8	73	theme	DMSO	1186:1189	arg1	treatment					1191:1199	DMSO treatment	1186:1199	DMSO treatment	1186:1199	Molecular size distributions and chain-length distributions of liver glycogen from both groups were characterized to test glycogen fragility before and after DMSO treatment.
34968551	9	74	theme	α	1361:1361	arg1	particles					1363:1371	liver-glycogen α particles	1346:1371	liver-glycogen α particles	1346:1371	Consistent with the hypothesis that high blood glucose is associated with glycogen fragility, a high-amylose RS diet prevented the fragility of liver-glycogen α particles.
34968551	0	75	theme	mice	82:85	arg1	structure					60:68	the glycogen structure	47:68	the glycogen structure of diabetic mice	47:85	The effect of high-amylose resistant starch on the glycogen structure of diabetic mice.
33802882	2	0	from	stability	202:210	arg1	ComPs					281:285	ComPs	281:285	ComPs	281:285	The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides was studied under the action of several factors.
33802882	2	0	from	stability	202:210	arg1	particles					270:278	complex particles	262:278	complex particles (ComPs)	262:286	The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides was studied under the action of several factors.
33802882	6	1	theme	significant	1041:1051	arg1	change					1053:1058	no significant change	1038:1058	no significant change	1038:1058	The total phenol (TPC) and total flavonoids (TFC) content values increased by 16% and 13%, respectively, for FC exposed to ultraviolet light at λ = 365 nm (UVA), whereas no significant change was observed for immobilized curcumin.
33802882	2	2	theme	several	355:361	arg1	factors					363:369	several factors	355:369	several factors	355:369	The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides was studied under the action of several factors.
33802882	9	3	theme	protective	1386:1395	arg1	role					1397:1400	a protective role	1384:1400	a protective role for the immobilized curcumin	1384:1429	ComPs have a protective role for the immobilized curcumin.
33802882	4	4	theme	degradation	641:651	arg1	t1/2					664:667	t1/2	664:667	t1/2	664:667	The degradation half-life (t1/2) decreases for FC when the pH increases, from 6.8 h at pH = 3 to 2.1 h at pH = 9.
33802882	4	4	theme	degradation	641:651	arg1	half-life					653:661	The degradation half-life	637:661	The degradation half-life (t1/2)	637:668	The degradation half-life (t1/2) decreases for FC when the pH increases, from 6.8 h at pH = 3 to 2.1 h at pH = 9.
33802882	6	5	dep	=	1014:1014	arg1	UVA					1024:1026	UVA	1024:1026	UVA	1024:1026	The total phenol (TPC) and total flavonoids (TFC) content values increased by 16% and 13%, respectively, for FC exposed to ultraviolet light at λ = 365 nm (UVA), whereas no significant change was observed for immobilized curcumin.
33802882	6	5	dep	=	1014:1014	arg1	nm					1020:1021	365 nm	1016:1021	365 nm (UVA)	1016:1027	The total phenol (TPC) and total flavonoids (TFC) content values increased by 16% and 13%, respectively, for FC exposed to ultraviolet light at λ = 365 nm (UVA), whereas no significant change was observed for immobilized curcumin.
33802882	8	6	theme	pH	1334:1335	arg1	value					1337:1341	the pH value	1330:1341	the pH value	1330:1341	The bovine serum albumin (BSA) adsorption efficiency on the ComPs surface depends on the pH value and the cross-linking degree.
33802882	0	7	theme	Curcumin	105:112	arg1	Bioactivity					78:88	Bioactivity	78:88	Bioactivity	78:88	Polysaccharides-Based Complex Particles' Protective Role on the Stability and Bioactivity of Immobilized Curcumin.
33802882	0	7	theme	Curcumin	105:112	arg1	Stability					64:72	Stability	64:72	Stability	64:72	Polysaccharides-Based Complex Particles' Protective Role on the Stability and Bioactivity of Immobilized Curcumin.
33802882	6	8	theme	ultraviolet	991:1001	arg1	light					1003:1007	ultraviolet light	991:1007	ultraviolet light	991:1007	The total phenol (TPC) and total flavonoids (TFC) content values increased by 16% and 13%, respectively, for FC exposed to ultraviolet light at λ = 365 nm (UVA), whereas no significant change was observed for immobilized curcumin.
33802882	3	9	theme	FC	524:525	arg1	FC					524:525	FC	524:525	FC	524:525	Ultraviolet-visible (UV-VIS) and Fourier-transform infrared (FTIR) spectroscopy proved the FC photodegradation and its role as a metal chelator: 82% of FC and between 26% and 39.79% of curcumin within the ComPs degraded after exposure for 28 days to natural light.
33802882	3	9	theme	FC	524:525	arg1	%					519:519	82%	517:519	82% of FC and between 26% and 39.79% of curcumin within the ComPs degraded after exposure for 28 days to natural light	517:634	Ultraviolet-visible (UV-VIS) and Fourier-transform infrared (FTIR) spectroscopy proved the FC photodegradation and its role as a metal chelator: 82% of FC and between 26% and 39.79% of curcumin within the ComPs degraded after exposure for 28 days to natural light.
33802882	0	10	theme	Immobilized	93:103	arg1	Curcumin					105:112	Immobilized Curcumin	93:112	Immobilized Curcumin	93:112	Polysaccharides-Based Complex Particles' Protective Role on the Stability and Bioactivity of Immobilized Curcumin.
33802882	8	11	theme	bovine	1249:1254	arg1	BSA					1271:1273	BSA	1271:1273	BSA	1271:1273	The bovine serum albumin (BSA) adsorption efficiency on the ComPs surface depends on the pH value and the cross-linking degree.
33802882	8	11	theme	bovine	1249:1254	arg1	albumin					1262:1268	bovine serum albumin	1249:1268	The bovine serum albumin (BSA) adsorption efficiency on the ComPs surface	1245:1317	The bovine serum albumin (BSA) adsorption efficiency on the ComPs surface depends on the pH value and the cross-linking degree.
33802882	3	12	theme	metal	501:505	arg1	chelator					507:514	a metal chelator	499:514	a metal chelator	499:514	Ultraviolet-visible (UV-VIS) and Fourier-transform infrared (FTIR) spectroscopy proved the FC photodegradation and its role as a metal chelator: 82% of FC and between 26% and 39.79% of curcumin within the ComPs degraded after exposure for 28 days to natural light.
33802882	4	13	from	pH	743:744	arg1	=					727:727	=	727:727	=	727:727	The degradation half-life (t1/2) decreases for FC when the pH increases, from 6.8 h at pH = 3 to 2.1 h at pH = 9.
33802882	9	14	contain	have	1379:1382	arg2	role					1397:1400	a protective role	1384:1400	a protective role for the immobilized curcumin	1384:1429	ComPs have a protective role for the immobilized curcumin.
33802882	9	14	contain	have	1379:1382	arg1	ComPs					1373:1377	ComPs	1373:1377	ComPs	1373:1377	ComPs have a protective role for the immobilized curcumin.
33802882	6	15	theme	total	895:899	arg1	TFC					913:915	TFC	913:915	TFC	913:915	The total phenol (TPC) and total flavonoids (TFC) content values increased by 16% and 13%, respectively, for FC exposed to ultraviolet light at λ = 365 nm (UVA), whereas no significant change was observed for immobilized curcumin.
33802882	6	15	theme	total	895:899	arg1	flavonoids					901:910	total flavonoids	895:910	total flavonoids (TFC)	895:916	The total phenol (TPC) and total flavonoids (TFC) content values increased by 16% and 13%, respectively, for FC exposed to ultraviolet light at λ = 365 nm (UVA), whereas no significant change was observed for immobilized curcumin.
33802882	4	16	dep	pH	724:725	arg1	=					727:727	=	727:727	=	727:727	The degradation half-life (t1/2) decreases for FC when the pH increases, from 6.8 h at pH = 3 to 2.1 h at pH = 9.
33802882	2	17	from	curcumin	220:227	arg1	ComPs					281:285	ComPs	281:285	ComPs	281:285	The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides was studied under the action of several factors.
33802882	2	17	from	curcumin	220:227	arg1	particles					270:278	complex particles	262:278	complex particles (ComPs)	262:286	The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides was studied under the action of several factors.
33802882	0	18	from	Role	52:55	arg1	Bioactivity					78:88	Bioactivity	78:88	Bioactivity	78:88	Polysaccharides-Based Complex Particles' Protective Role on the Stability and Bioactivity of Immobilized Curcumin.
33802882	0	18	from	Role	52:55	arg1	Stability					64:72	Stability	64:72	Stability	64:72	Polysaccharides-Based Complex Particles' Protective Role on the Stability and Bioactivity of Immobilized Curcumin.
33802882	6	19	theme	flavonoids	901:910	arg1	values					926:931	The total phenol (TPC) and total flavonoids (TFC) content values	868:931	The total phenol (TPC) and total flavonoids (TFC) content values	868:931	The total phenol (TPC) and total flavonoids (TFC) content values increased by 16% and 13%, respectively, for FC exposed to ultraviolet light at λ = 365 nm (UVA), whereas no significant change was observed for immobilized curcumin.
33802882	0	20	theme	Complex	22:28	arg1	Particles					30:38	Polysaccharides-Based Complex Particles	0:38	Polysaccharides-Based Complex Particles	0:38	Polysaccharides-Based Complex Particles' Protective Role on the Stability and Bioactivity of Immobilized Curcumin.
33802882	8	21	theme	serum	1256:1260	arg1	BSA					1271:1273	BSA	1271:1273	BSA	1271:1273	The bovine serum albumin (BSA) adsorption efficiency on the ComPs surface depends on the pH value and the cross-linking degree.
33802882	8	21	theme	serum	1256:1260	arg1	albumin					1262:1268	bovine serum albumin	1249:1268	The bovine serum albumin (BSA) adsorption efficiency on the ComPs surface	1245:1317	The bovine serum albumin (BSA) adsorption efficiency on the ComPs surface depends on the pH value and the cross-linking degree.
33802882	4	22	theme	=	746:746	arg1	pH					743:744	pH	743:744	pH = 9	743:748	The degradation half-life (t1/2) decreases for FC when the pH increases, from 6.8 h at pH = 3 to 2.1 h at pH = 9.
33802882	3	23	theme	FTIR	433:436	arg1	spectroscopy					439:450	Ultraviolet-visible (UV-VIS) and Fourier-transform infrared (FTIR) spectroscopy	372:450	spectroscopy	439:450	Ultraviolet-visible (UV-VIS) and Fourier-transform infrared (FTIR) spectroscopy proved the FC photodegradation and its role as a metal chelator: 82% of FC and between 26% and 39.79% of curcumin within the ComPs degraded after exposure for 28 days to natural light.
33802882	0	24	theme	Polysaccharides-Based	0:20	arg1	Particles					30:38	Polysaccharides-Based Complex Particles	0:38	Polysaccharides-Based Complex Particles	0:38	Polysaccharides-Based Complex Particles' Protective Role on the Stability and Bioactivity of Immobilized Curcumin.
33802882	8	25	from	efficiency	1287:1296	arg1	surface					1311:1317	the ComPs surface	1301:1317	the ComPs surface	1301:1317	The bovine serum albumin (BSA) adsorption efficiency on the ComPs surface depends on the pH value and the cross-linking degree.
33802882	2	26	theme	different	297:305	arg1	polysaccharides					307:321	different polysaccharides	297:321	different polysaccharides	297:321	The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides was studied under the action of several factors.
33802882	2	27	from	curcumin	250:257	arg1	ComPs					281:285	ComPs	281:285	ComPs	281:285	The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides was studied under the action of several factors.
33802882	2	27	from	curcumin	250:257	arg1	particles					270:278	complex particles	262:278	complex particles (ComPs)	262:286	The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides was studied under the action of several factors.
33802882	4	28	dep	2.1	734:736	arg1	to					731:732	to	731:732	to	731:732	The degradation half-life (t1/2) decreases for FC when the pH increases, from 6.8 h at pH = 3 to 2.1 h at pH = 9.
33802882	8	29	theme	adsorption	1276:1285	arg1	efficiency					1287:1296	The bovine serum albumin (BSA) adsorption efficiency	1245:1296	The bovine serum albumin (BSA) adsorption efficiency on the ComPs surface	1245:1317	The bovine serum albumin (BSA) adsorption efficiency on the ComPs surface depends on the pH value and the cross-linking degree.
33802882	0	30	dep	Stability	64:72	arg1	the					60:62	the	60:62	the	60:62	Polysaccharides-Based Complex Particles' Protective Role on the Stability and Bioactivity of Immobilized Curcumin.
33802882	2	31	from	particles	270:278	arg1	stability					202:210	The stability	198:210	The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides	198:321	The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides was studied under the action of several factors.
33802882	3	32	theme	infrared	423:430	arg1	spectroscopy					439:450	Ultraviolet-visible (UV-VIS) and Fourier-transform infrared (FTIR) spectroscopy	372:450	spectroscopy	439:450	Ultraviolet-visible (UV-VIS) and Fourier-transform infrared (FTIR) spectroscopy proved the FC photodegradation and its role as a metal chelator: 82% of FC and between 26% and 39.79% of curcumin within the ComPs degraded after exposure for 28 days to natural light.
33802882	9	33	theme	immobilized	1410:1420	arg1	curcumin					1422:1429	the immobilized curcumin	1406:1429	the immobilized curcumin	1406:1429	ComPs have a protective role for the immobilized curcumin.
33802882	2	34	theme	immobilized	238:248	arg1	curcumin					250:257	immobilized curcumin	238:257	immobilized curcumin in complex particles (ComPs)	238:286	The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides was studied under the action of several factors.
33802882	0	35	theme	Protective	41:50	arg1	Role					52:55	' Protective Role	39:55	' Protective Role	39:55	Polysaccharides-Based Complex Particles' Protective Role on the Stability and Bioactivity of Immobilized Curcumin.
33802882	6	36	theme	immobilized	1077:1087	arg1	curcumin					1089:1096	immobilized curcumin	1077:1096	immobilized curcumin	1077:1096	The total phenol (TPC) and total flavonoids (TFC) content values increased by 16% and 13%, respectively, for FC exposed to ultraviolet light at λ = 365 nm (UVA), whereas no significant change was observed for immobilized curcumin.
33802882	3	37	theme	curcumin	557:564	arg1	curcumin					557:564	curcumin	557:564	curcumin	557:564	Ultraviolet-visible (UV-VIS) and Fourier-transform infrared (FTIR) spectroscopy proved the FC photodegradation and its role as a metal chelator: 82% of FC and between 26% and 39.79% of curcumin within the ComPs degraded after exposure for 28 days to natural light.
33802882	3	37	theme	curcumin	557:564	arg1	%					541:541	26%	539:541	26%	539:541	Ultraviolet-visible (UV-VIS) and Fourier-transform infrared (FTIR) spectroscopy proved the FC photodegradation and its role as a metal chelator: 82% of FC and between 26% and 39.79% of curcumin within the ComPs degraded after exposure for 28 days to natural light.
33802882	3	37	theme	curcumin	557:564	arg1	%					552:552	39.79%	547:552	39.79%	547:552	Ultraviolet-visible (UV-VIS) and Fourier-transform infrared (FTIR) spectroscopy proved the FC photodegradation and its role as a metal chelator: 82% of FC and between 26% and 39.79% of curcumin within the ComPs degraded after exposure for 28 days to natural light.
33802882	6	38	dep	increased	933:941	arg1	whereas					1030:1036	whereas	1030:1036	whereas	1030:1036	The total phenol (TPC) and total flavonoids (TFC) content values increased by 16% and 13%, respectively, for FC exposed to ultraviolet light at λ = 365 nm (UVA), whereas no significant change was observed for immobilized curcumin.
33802882	3	39	theme	FC	463:464	arg1	photodegradation					466:481	the FC photodegradation	459:481	the FC photodegradation	459:481	Ultraviolet-visible (UV-VIS) and Fourier-transform infrared (FTIR) spectroscopy proved the FC photodegradation and its role as a metal chelator: 82% of FC and between 26% and 39.79% of curcumin within the ComPs degraded after exposure for 28 days to natural light.
33802882	5	40	dep	constant	795:802	arg1	h					823:823	10 and 13 h	813:823	10 and 13 h	813:823	For curcumin extracted from ComPs, t1/2 was constant (between 10 and 13 h) and depended on the sample's composition.
33802882	8	41	theme	albumin	1262:1268	arg1	efficiency					1287:1296	The bovine serum albumin (BSA) adsorption efficiency	1245:1296	The bovine serum albumin (BSA) adsorption efficiency on the ComPs surface	1245:1317	The bovine serum albumin (BSA) adsorption efficiency on the ComPs surface depends on the pH value and the cross-linking degree.
33802882	1	42	theme	curcumin	119:126	arg1	degradation					128:138	The curcumin degradation	115:138	The curcumin degradation	115:138	The curcumin degradation represents a significant limitation for its applications.
33802882	3	43	theme	Fourier-transform	405:421	arg1	spectroscopy					439:450	Ultraviolet-visible (UV-VIS) and Fourier-transform infrared (FTIR) spectroscopy	372:450	spectroscopy	439:450	Ultraviolet-visible (UV-VIS) and Fourier-transform infrared (FTIR) spectroscopy proved the FC photodegradation and its role as a metal chelator: 82% of FC and between 26% and 39.79% of curcumin within the ComPs degraded after exposure for 28 days to natural light.
33802882	8	44	theme	ComPs	1305:1309	arg1	surface					1311:1317	the ComPs surface	1301:1317	the ComPs surface	1301:1317	The bovine serum albumin (BSA) adsorption efficiency on the ComPs surface depends on the pH value and the cross-linking degree.
33802882	3	45	theme	natural	622:628	arg1	light					630:634	natural light	622:634	natural light	622:634	Ultraviolet-visible (UV-VIS) and Fourier-transform infrared (FTIR) spectroscopy proved the FC photodegradation and its role as a metal chelator: 82% of FC and between 26% and 39.79% of curcumin within the ComPs degraded after exposure for 28 days to natural light.
33802882	3	46	dep	proved	452:457	arg1	FC					524:525	FC	524:525	FC	524:525	Ultraviolet-visible (UV-VIS) and Fourier-transform infrared (FTIR) spectroscopy proved the FC photodegradation and its role as a metal chelator: 82% of FC and between 26% and 39.79% of curcumin within the ComPs degraded after exposure for 28 days to natural light.
33802882	3	46	dep	proved	452:457	arg1	%					519:519	82%	517:519	82% of FC and between 26% and 39.79% of curcumin within the ComPs degraded after exposure for 28 days to natural light	517:634	Ultraviolet-visible (UV-VIS) and Fourier-transform infrared (FTIR) spectroscopy proved the FC photodegradation and its role as a metal chelator: 82% of FC and between 26% and 39.79% of curcumin within the ComPs degraded after exposure for 28 days to natural light.
33802882	7	47	theme	Antioxidant	1099:1109	arg1	activity					1111:1118	Antioxidant activity	1099:1118	Antioxidant activity expressed by IC50 (µmoles/mL) for FC exposed to UVA	1099:1170	Antioxidant activity expressed by IC50 (µmoles/mL) for FC exposed to UVA decreased by 29%, but curcumin within ComPs was not affected by the UVA.
33802882	2	48	theme	curcumin	220:227	arg1	stability					202:210	The stability	198:210	The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides	198:321	The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides was studied under the action of several factors.
33802882	6	49	theme	=	1014:1014	arg1	λ					1012:1012	λ	1012:1012	λ = 365 nm (UVA)	1012:1027	The total phenol (TPC) and total flavonoids (TFC) content values increased by 16% and 13%, respectively, for FC exposed to ultraviolet light at λ = 365 nm (UVA), whereas no significant change was observed for immobilized curcumin.
33802882	8	50	theme	cross-linking	1351:1363	arg1	degree					1365:1370	the cross-linking degree	1347:1370	the cross-linking degree	1347:1370	The bovine serum albumin (BSA) adsorption efficiency on the ComPs surface depends on the pH value and the cross-linking degree.
33802882	6	51	theme	total	872:876	arg1	TPC					886:888	TPC	886:888	TPC	886:888	The total phenol (TPC) and total flavonoids (TFC) content values increased by 16% and 13%, respectively, for FC exposed to ultraviolet light at λ = 365 nm (UVA), whereas no significant change was observed for immobilized curcumin.
33802882	6	51	theme	total	872:876	arg1	phenol					878:883	The total phenol	868:883	The total phenol (TPC)	868:889	The total phenol (TPC) and total flavonoids (TFC) content values increased by 16% and 13%, respectively, for FC exposed to ultraviolet light at λ = 365 nm (UVA), whereas no significant change was observed for immobilized curcumin.
33802882	2	52	theme	free	215:218	arg1	FC					230:231	FC	230:231	FC	230:231	The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides was studied under the action of several factors.
33802882	2	52	theme	free	215:218	arg1	curcumin					220:227	free curcumin	215:227	free curcumin (FC)	215:232	The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides was studied under the action of several factors.
33802882	2	53	theme	complex	262:268	arg1	ComPs					281:285	ComPs	281:285	ComPs	281:285	The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides was studied under the action of several factors.
33802882	2	53	theme	complex	262:268	arg1	particles					270:278	complex particles	262:278	complex particles (ComPs)	262:286	The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides was studied under the action of several factors.
33802882	4	54	from	pH	724:725	arg1	h					719:719	6.8 h	715:719	6.8 h at pH = 3 to 2.1 h at pH = 9	715:748	The degradation half-life (t1/2) decreases for FC when the pH increases, from 6.8 h at pH = 3 to 2.1 h at pH = 9.
33802882	6	55	theme	content	918:924	arg1	values					926:931	The total phenol (TPC) and total flavonoids (TFC) content values	868:931	The total phenol (TPC) and total flavonoids (TFC) content values	868:931	The total phenol (TPC) and total flavonoids (TFC) content values increased by 16% and 13%, respectively, for FC exposed to ultraviolet light at λ = 365 nm (UVA), whereas no significant change was observed for immobilized curcumin.
33802882	6	56	theme	phenol	878:883	arg1	values					926:931	The total phenol (TPC) and total flavonoids (TFC) content values	868:931	The total phenol (TPC) and total flavonoids (TFC) content values	868:931	The total phenol (TPC) and total flavonoids (TFC) content values increased by 16% and 13%, respectively, for FC exposed to ultraviolet light at λ = 365 nm (UVA), whereas no significant change was observed for immobilized curcumin.
33802882	1	57	theme	significant	153:163	arg1	limitation					165:174	a significant limitation	151:174	a significant limitation for its applications	151:195	The curcumin degradation represents a significant limitation for its applications.
33802882	4	58	dep	=	746:746	arg1	9					748:748	9	748:748	9	748:748	The degradation half-life (t1/2) decreases for FC when the pH increases, from 6.8 h at pH = 3 to 2.1 h at pH = 9.
33802882	2	59	theme	curcumin	250:257	arg1	stability					202:210	The stability	198:210	The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides	198:321	The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides was studied under the action of several factors.
33802882	2	60	theme	factors	363:369	arg1	action					345:350	the action	341:350	the action of several factors	341:369	The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides was studied under the action of several factors.
34134408	6	0	theme	grafting	845:852	arg1	α-cellulose					854:864	grafting α-cellulose	845:864	grafting α-cellulose with PHB	845:873	Thereafter, grafting α-cellulose with PHB offered intermolecular bonding, which resulted in enhanced thermal stability of the biocomposite than corresponding pristine PHB.
34134408	2	1	theme	fatty	376:380	arg1	acids					382:386	volatile fatty acids	367:386	volatile fatty acids (VFA) derived from vegetable waste	367:421	PHB was produced using dark fermentation effluent rich in volatile fatty acids (VFA) derived from vegetable waste and α-cellulose was extracted from sugarcane bagasse (SCB).
34134408	2	1	theme	fatty	376:380	arg1	VFA					389:391	VFA	389:391	VFA	389:391	PHB was produced using dark fermentation effluent rich in volatile fatty acids (VFA) derived from vegetable waste and α-cellulose was extracted from sugarcane bagasse (SCB).
34134408	8	2	theme	effective	1227:1235	arg1	agent					1249:1253	an effective reinforcing agent	1224:1253	an effective reinforcing agent	1224:1253	XRD profiles showed significant decrement in PHB crystallinity, signifying the functional role of α-cellulose as an effective reinforcing agent.
34134408	11	3	theme	acidogenic	1725:1734	arg1	effluents					1749:1757	acidogenic fermentation effluents	1725:1757	acidogenic fermentation effluents	1725:1757	Additional value from this process was further achieved by incorporating the concept of biorefinery, wherein acidogenic fermentation effluents were used for the production of PHA, which enabled the re-entry of products (VFA) to the production cycle, thus achieving circularity.
34134408	11	4	theme	PHA	1791:1793	arg1	production					1777:1786	the production	1773:1786	the production	1773:1786	Additional value from this process was further achieved by incorporating the concept of biorefinery, wherein acidogenic fermentation effluents were used for the production of PHA, which enabled the re-entry of products (VFA) to the production cycle, thus achieving circularity.
34134408	3	5	theme	0.28 ± 0.06 g	569:581	arg1	DCW					589:591	0.28 ± 0.06 g PHB/g DCW	569:591	0.28 ± 0.06 g PHB/g DCW (28%)	569:597	Nutrient limitation induced microbial PHB accumulation, wherein maximum production of 0.28 ± 0.06 g PHB/g DCW (28%) was observed.
34134408	3	5	theme	0.28 ± 0.06 g	569:581	arg1	%					596:596	28%	594:596	28%	594:596	Nutrient limitation induced microbial PHB accumulation, wherein maximum production of 0.28 ± 0.06 g PHB/g DCW (28%) was observed.
34134408	1	6	theme	biorefinery	287:297	arg1	approach					299:306	a biorefinery approach	285:306	a biorefinery approach	285:306	The study evaluated the preparation of a biocomposite using waste-derived polyhydroxybutyrate (PHB) and bagasse cellulose (α-cellulose) in a biorefinery approach.
34134408	6	7	theme	enhanced	925:932	arg1	stability					942:950	enhanced thermal stability	925:950	enhanced thermal stability of the biocomposite than corresponding pristine PHB	925:1002	Thereafter, grafting α-cellulose with PHB offered intermolecular bonding, which resulted in enhanced thermal stability of the biocomposite than corresponding pristine PHB.
34134408	10	8	theme	produced	1515:1522	arg1	expands					1528:1534	biologically produced PHB expands	1502:1534	biologically produced PHB expands	1502:1534	Utilization of bagasse-derived cellulose for strengthening biologically produced PHB expands its applications, while simultaneously addressing the plastic pollution issues.
34134408	11	9	used	used	1764:1767	arg2	effluents					1749:1757	acidogenic fermentation effluents	1725:1757	acidogenic fermentation effluents	1725:1757	Additional value from this process was further achieved by incorporating the concept of biorefinery, wherein acidogenic fermentation effluents were used for the production of PHA, which enabled the re-entry of products (VFA) to the production cycle, thus achieving circularity.
34134408	4	10	theme	cell	679:682	arg1	cytoplasm					684:692	the cell cytoplasm	675:692	the cell cytoplasm	675:692	Confocal examination showed the deposition of PHB granules in the cell cytoplasm and NMR spectrum exhibited a structural correlation.
34134408	2	11	theme	dark	332:335	arg1	effluent					350:357	dark fermentation effluent	332:357	dark fermentation effluent rich in volatile fatty acids (VFA) derived from vegetable waste	332:421	PHB was produced using dark fermentation effluent rich in volatile fatty acids (VFA) derived from vegetable waste and α-cellulose was extracted from sugarcane bagasse (SCB).
34134408	10	12	theme	cellulose	1474:1482	arg1	Utilization					1443:1453	Utilization	1443:1453	Utilization of bagasse-derived cellulose for strengthening biologically produced PHB expands	1443:1534	Utilization of bagasse-derived cellulose for strengthening biologically produced PHB expands its applications, while simultaneously addressing the plastic pollution issues.
34134408	10	13	link	bagasse-derived	1458:1472	arg1	cellulose					1474:1482	bagasse-derived cellulose	1458:1482	bagasse-derived cellulose	1458:1482	Utilization of bagasse-derived cellulose for strengthening biologically produced PHB expands its applications, while simultaneously addressing the plastic pollution issues.
34134408	3	14	theme	DCW	589:591	arg1	production					555:564	wherein maximum production	539:564	wherein maximum production of 0.28 ± 0.06 g PHB/g DCW (28%)	539:597	Nutrient limitation induced microbial PHB accumulation, wherein maximum production of 0.28 ± 0.06 g PHB/g DCW (28%) was observed.
34134408	4	15	from	deposition	645:654	arg1	cytoplasm					684:692	the cell cytoplasm	675:692	the cell cytoplasm	675:692	Confocal examination showed the deposition of PHB granules in the cell cytoplasm and NMR spectrum exhibited a structural correlation.
34134408	4	16	theme	PHB	659:661	arg1	granules					663:670	PHB granules	659:670	PHB granules	659:670	Confocal examination showed the deposition of PHB granules in the cell cytoplasm and NMR spectrum exhibited a structural correlation.
34134408	6	17	theme	pristine	991:998	arg1	PHB					1000:1002	corresponding pristine PHB	977:1002	corresponding pristine PHB	977:1002	Thereafter, grafting α-cellulose with PHB offered intermolecular bonding, which resulted in enhanced thermal stability of the biocomposite than corresponding pristine PHB.
34134408	9	18	theme	PHB	1327:1329	arg1	group					1318:1322	ester group	1312:1322	ester group of PHB	1312:1329	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	9	18	theme	PHB	1327:1329	arg1	group					1287:1291	ether functional group	1270:1291	ether functional group of α-cellulose	1270:1306	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	9	18	theme	PHB	1327:1329	arg1	PHB					1327:1329	PHB	1327:1329	PHB	1327:1329	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	9	18	theme	PHB	1327:1329	arg1	α-cellulose					1296:1306	α-cellulose	1296:1306	α-cellulose	1296:1306	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	9	19	theme	XPS	1348:1350	arg1	analysis					1352:1359	XPS analysis	1348:1359	XPS analysis of the composite	1348:1376	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	11	20	from	process	1643:1649	arg1	value					1627:1631	Additional value	1616:1631	Additional value from this process	1616:1649	Additional value from this process was further achieved by incorporating the concept of biorefinery, wherein acidogenic fermentation effluents were used for the production of PHA, which enabled the re-entry of products (VFA) to the production cycle, thus achieving circularity.
34134408	7	21	theme	morphological	1012:1024	arg1	examination					1026:1036	FE-SEM morphological examination	1005:1036	FE-SEM morphological examination of biocomposite	1005:1052	FE-SEM morphological examination of biocomposite depicted that α-cellulose functioned as a filler to PHB.
34134408	11	22	theme	Additional	1616:1625	arg1	value					1627:1631	Additional value	1616:1631	Additional value from this process	1616:1649	Additional value from this process was further achieved by incorporating the concept of biorefinery, wherein acidogenic fermentation effluents were used for the production of PHA, which enabled the re-entry of products (VFA) to the production cycle, thus achieving circularity.
34134408	9	23	theme	composite	1368:1376	arg1	analysis					1352:1359	XPS analysis	1348:1359	XPS analysis of the composite	1348:1376	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	1	24	link	waste-derived	206:218	arg1	PHB					241:243	PHB	241:243	PHB	241:243	The study evaluated the preparation of a biocomposite using waste-derived polyhydroxybutyrate (PHB) and bagasse cellulose (α-cellulose) in a biorefinery approach.
34134408	1	24	link	waste-derived	206:218	arg1	polyhydroxybutyrate					220:238	waste-derived polyhydroxybutyrate	206:238	waste-derived polyhydroxybutyrate (PHB)	206:244	The study evaluated the preparation of a biocomposite using waste-derived polyhydroxybutyrate (PHB) and bagasse cellulose (α-cellulose) in a biorefinery approach.
34134408	2	25	theme	vegetable	407:415	arg1	waste					417:421	vegetable waste	407:421	vegetable waste	407:421	PHB was produced using dark fermentation effluent rich in volatile fatty acids (VFA) derived from vegetable waste and α-cellulose was extracted from sugarcane bagasse (SCB).
34134408	0	26	theme	biorefinery	124:134	arg1	approach					136:143	a biorefinery approach	122:143	a biorefinery approach	122:143	Polyhydroxybutyrate production from dark-fermentative effluent and composite grafting with bagasse derived α-cellulose in a biorefinery approach.
34134408	8	27	theme	XRD	1111:1113	arg1	profiles					1115:1122	XRD profiles	1111:1122	XRD profiles	1111:1122	XRD profiles showed significant decrement in PHB crystallinity, signifying the functional role of α-cellulose as an effective reinforcing agent.
34134408	0	28	from	effluent	54:61	arg1	production					20:29	Polyhydroxybutyrate production	0:29	Polyhydroxybutyrate production from dark-fermentative effluent and composite grafting with bagasse	0:97	Polyhydroxybutyrate production from dark-fermentative effluent and composite grafting with bagasse derived α-cellulose in a biorefinery approach.
34134408	0	29	theme	Polyhydroxybutyrate	0:18	arg1	production					20:29	Polyhydroxybutyrate production	0:29	Polyhydroxybutyrate production from dark-fermentative effluent and composite grafting with bagasse	0:97	Polyhydroxybutyrate production from dark-fermentative effluent and composite grafting with bagasse derived α-cellulose in a biorefinery approach.
34134408	6	30	with	α-cellulose	854:864	arg1	PHB					871:873	PHB	871:873	PHB	871:873	Thereafter, grafting α-cellulose with PHB offered intermolecular bonding, which resulted in enhanced thermal stability of the biocomposite than corresponding pristine PHB.
34134408	10	31	theme	plastic	1590:1596	arg1	issues					1608:1613	the plastic pollution issues	1586:1613	the plastic pollution issues	1586:1613	Utilization of bagasse-derived cellulose for strengthening biologically produced PHB expands its applications, while simultaneously addressing the plastic pollution issues.
34134408	9	32	theme	α-cellulose	1422:1432	arg1	blending					1410:1417	the effective blending	1396:1417	the effective blending of α-cellulose and PHB	1396:1440	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	8	33	theme	PHB	1156:1158	arg1	crystallinity					1160:1172	PHB crystallinity	1156:1172	PHB crystallinity	1156:1172	XRD profiles showed significant decrement in PHB crystallinity, signifying the functional role of α-cellulose as an effective reinforcing agent.
34134408	2	34	theme	volatile	367:374	arg1	acids					382:386	volatile fatty acids	367:386	volatile fatty acids (VFA) derived from vegetable waste	367:421	PHB was produced using dark fermentation effluent rich in volatile fatty acids (VFA) derived from vegetable waste and α-cellulose was extracted from sugarcane bagasse (SCB).
34134408	2	34	theme	volatile	367:374	arg1	VFA					389:391	VFA	389:391	VFA	389:391	PHB was produced using dark fermentation effluent rich in volatile fatty acids (VFA) derived from vegetable waste and α-cellulose was extracted from sugarcane bagasse (SCB).
34134408	0	35	theme	composite	67:75	arg1	grafting					77:84	composite grafting	67:84	composite grafting	67:84	Polyhydroxybutyrate production from dark-fermentative effluent and composite grafting with bagasse derived α-cellulose in a biorefinery approach.
34134408	9	36	theme	PHB	1438:1440	arg1	blending					1410:1417	the effective blending	1396:1417	the effective blending of α-cellulose and PHB	1396:1440	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	3	37	theme	Nutrient	483:490	arg1	limitation					492:501	Nutrient limitation	483:501	Nutrient limitation	483:501	Nutrient limitation induced microbial PHB accumulation, wherein maximum production of 0.28 ± 0.06 g PHB/g DCW (28%) was observed.
34134408	9	38	theme	functional	1276:1285	arg1	group					1287:1291	ether functional group	1270:1291	ether functional group of α-cellulose	1270:1306	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	9	38	theme	functional	1276:1285	arg1	PHB					1327:1329	PHB	1327:1329	PHB	1327:1329	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	9	38	theme	functional	1276:1285	arg1	α-cellulose					1296:1306	α-cellulose	1296:1306	α-cellulose	1296:1306	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	2	39	theme	rich	359:362	arg1	effluent					350:357	dark fermentation effluent	332:357	dark fermentation effluent rich in volatile fatty acids (VFA) derived from vegetable waste	332:421	PHB was produced using dark fermentation effluent rich in volatile fatty acids (VFA) derived from vegetable waste and α-cellulose was extracted from sugarcane bagasse (SCB).
34134408	1	40	theme	bagasse	250:256	arg1	α-cellulose					269:279	α-cellulose	269:279	α-cellulose	269:279	The study evaluated the preparation of a biocomposite using waste-derived polyhydroxybutyrate (PHB) and bagasse cellulose (α-cellulose) in a biorefinery approach.
34134408	1	40	theme	bagasse	250:256	arg1	cellulose					258:266	bagasse cellulose	250:266	bagasse cellulose (α-cellulose)	250:280	The study evaluated the preparation of a biocomposite using waste-derived polyhydroxybutyrate (PHB) and bagasse cellulose (α-cellulose) in a biorefinery approach.
34134408	11	41	theme	products	1826:1833	arg1	re-entry					1814:1821	the re-entry	1810:1821	the re-entry of products (VFA) to the production cycle	1810:1863	Additional value from this process was further achieved by incorporating the concept of biorefinery, wherein acidogenic fermentation effluents were used for the production of PHA, which enabled the re-entry of products (VFA) to the production cycle, thus achieving circularity.
34134408	6	42	theme	biocomposite	959:970	arg1	stability					942:950	enhanced thermal stability	925:950	enhanced thermal stability of the biocomposite than corresponding pristine PHB	925:1002	Thereafter, grafting α-cellulose with PHB offered intermolecular bonding, which resulted in enhanced thermal stability of the biocomposite than corresponding pristine PHB.
34134408	0	43	with	effluent	54:61	arg1	bagasse					91:97	bagasse	91:97	bagasse	91:97	Polyhydroxybutyrate production from dark-fermentative effluent and composite grafting with bagasse derived α-cellulose in a biorefinery approach.
34134408	3	44	theme	PHB	521:523	arg1	accumulation					525:536	microbial PHB accumulation	511:536	microbial PHB accumulation	511:536	Nutrient limitation induced microbial PHB accumulation, wherein maximum production of 0.28 ± 0.06 g PHB/g DCW (28%) was observed.
34134408	8	45	theme	α-cellulose	1209:1219	arg1	role					1201:1204	the functional role	1186:1204	the functional role of α-cellulose as an effective reinforcing agent	1186:1253	XRD profiles showed significant decrement in PHB crystallinity, signifying the functional role of α-cellulose as an effective reinforcing agent.
34134408	2	46	from	rich	359:362	arg1	acids					382:386	volatile fatty acids	367:386	volatile fatty acids (VFA) derived from vegetable waste	367:421	PHB was produced using dark fermentation effluent rich in volatile fatty acids (VFA) derived from vegetable waste and α-cellulose was extracted from sugarcane bagasse (SCB).
34134408	2	46	from	rich	359:362	arg1	VFA					389:391	VFA	389:391	VFA	389:391	PHB was produced using dark fermentation effluent rich in volatile fatty acids (VFA) derived from vegetable waste and α-cellulose was extracted from sugarcane bagasse (SCB).
34134408	10	47	theme	bagasse-derived	1458:1472	arg1	cellulose					1474:1482	bagasse-derived cellulose	1458:1482	bagasse-derived cellulose	1458:1482	Utilization of bagasse-derived cellulose for strengthening biologically produced PHB expands its applications, while simultaneously addressing the plastic pollution issues.
34134408	6	48	theme	thermal	934:940	arg1	stability					942:950	enhanced thermal stability	925:950	enhanced thermal stability of the biocomposite than corresponding pristine PHB	925:1002	Thereafter, grafting α-cellulose with PHB offered intermolecular bonding, which resulted in enhanced thermal stability of the biocomposite than corresponding pristine PHB.
34134408	9	49	theme	ether	1270:1274	arg1	group					1287:1291	ether functional group	1270:1291	ether functional group of α-cellulose	1270:1306	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	9	49	theme	ether	1270:1274	arg1	PHB					1327:1329	PHB	1327:1329	PHB	1327:1329	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	9	49	theme	ether	1270:1274	arg1	α-cellulose					1296:1306	α-cellulose	1296:1306	α-cellulose	1296:1306	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	3	50	theme	maximum	547:553	arg1	production					555:564	wherein maximum production	539:564	wherein maximum production of 0.28 ± 0.06 g PHB/g DCW (28%)	539:597	Nutrient limitation induced microbial PHB accumulation, wherein maximum production of 0.28 ± 0.06 g PHB/g DCW (28%) was observed.
34134408	1	51	theme	biocomposite	187:198	arg1	preparation					170:180	the preparation	166:180	the preparation of a biocomposite	166:198	The study evaluated the preparation of a biocomposite using waste-derived polyhydroxybutyrate (PHB) and bagasse cellulose (α-cellulose) in a biorefinery approach.
34134408	8	52	theme	reinforcing	1237:1247	arg1	agent					1249:1253	an effective reinforcing agent	1224:1253	an effective reinforcing agent	1224:1253	XRD profiles showed significant decrement in PHB crystallinity, signifying the functional role of α-cellulose as an effective reinforcing agent.
34134408	2	53	from	acids	382:386	arg1	rich					359:362	rich	359:362	rich	359:362	PHB was produced using dark fermentation effluent rich in volatile fatty acids (VFA) derived from vegetable waste and α-cellulose was extracted from sugarcane bagasse (SCB).
34134408	4	54	theme	granules	663:670	arg1	deposition					645:654	the deposition	641:654	the deposition of PHB granules in the cell cytoplasm	641:692	Confocal examination showed the deposition of PHB granules in the cell cytoplasm and NMR spectrum exhibited a structural correlation.
34134408	2	55	theme	fermentation	337:348	arg1	effluent					350:357	dark fermentation effluent	332:357	dark fermentation effluent rich in volatile fatty acids (VFA) derived from vegetable waste	332:421	PHB was produced using dark fermentation effluent rich in volatile fatty acids (VFA) derived from vegetable waste and α-cellulose was extracted from sugarcane bagasse (SCB).
34134408	1	56	theme	waste-derived	206:218	arg1	PHB					241:243	PHB	241:243	PHB	241:243	The study evaluated the preparation of a biocomposite using waste-derived polyhydroxybutyrate (PHB) and bagasse cellulose (α-cellulose) in a biorefinery approach.
34134408	1	56	theme	waste-derived	206:218	arg1	polyhydroxybutyrate					220:238	waste-derived polyhydroxybutyrate	206:238	waste-derived polyhydroxybutyrate (PHB)	206:244	The study evaluated the preparation of a biocomposite using waste-derived polyhydroxybutyrate (PHB) and bagasse cellulose (α-cellulose) in a biorefinery approach.
34134408	11	57	dep	wherein	1717:1723	arg1	used					1764:1767	used	1764:1767	were used for the production of PHA, which enabled the re-entry of products (VFA) to the production cycle, thus achieving circularity	1759:1891	Additional value from this process was further achieved by incorporating the concept of biorefinery, wherein acidogenic fermentation effluents were used for the production of PHA, which enabled the re-entry of products (VFA) to the production cycle, thus achieving circularity.
34134408	9	58	theme	α-cellulose	1296:1306	arg1	group					1318:1322	ester group	1312:1322	ester group of PHB	1312:1329	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	9	58	theme	α-cellulose	1296:1306	arg1	group					1287:1291	ether functional group	1270:1291	ether functional group of α-cellulose	1270:1306	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	9	58	theme	α-cellulose	1296:1306	arg1	PHB					1327:1329	PHB	1327:1329	PHB	1327:1329	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	9	58	theme	α-cellulose	1296:1306	arg1	α-cellulose					1296:1306	α-cellulose	1296:1306	α-cellulose	1296:1306	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	3	59	theme	PHB/g	583:587	arg1	DCW					589:591	0.28 ± 0.06 g PHB/g DCW	569:591	0.28 ± 0.06 g PHB/g DCW (28%)	569:597	Nutrient limitation induced microbial PHB accumulation, wherein maximum production of 0.28 ± 0.06 g PHB/g DCW (28%) was observed.
34134408	3	59	theme	PHB/g	583:587	arg1	%					596:596	28%	594:596	28%	594:596	Nutrient limitation induced microbial PHB accumulation, wherein maximum production of 0.28 ± 0.06 g PHB/g DCW (28%) was observed.
34134408	0	60	from	α-cellulose	107:117	arg1	approach					136:143	a biorefinery approach	122:143	a biorefinery approach	122:143	Polyhydroxybutyrate production from dark-fermentative effluent and composite grafting with bagasse derived α-cellulose in a biorefinery approach.
34134408	9	61	theme	ester	1312:1316	arg1	group					1318:1322	ester group	1312:1322	ester group of PHB	1312:1329	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	9	61	theme	ester	1312:1316	arg1	PHB					1327:1329	PHB	1327:1329	PHB	1327:1329	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	9	61	theme	ester	1312:1316	arg1	α-cellulose					1296:1306	α-cellulose	1296:1306	α-cellulose	1296:1306	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	6	62	theme	corresponding	977:989	arg1	PHB					1000:1002	corresponding pristine PHB	977:1002	corresponding pristine PHB	977:1002	Thereafter, grafting α-cellulose with PHB offered intermolecular bonding, which resulted in enhanced thermal stability of the biocomposite than corresponding pristine PHB.
34134408	4	63	theme	structural	723:732	arg1	correlation					734:744	a structural correlation	721:744	a structural correlation	721:744	Confocal examination showed the deposition of PHB granules in the cell cytoplasm and NMR spectrum exhibited a structural correlation.
34134408	11	64	theme	production	1848:1857	arg1	cycle					1859:1863	the production cycle	1844:1863	the production cycle	1844:1863	Additional value from this process was further achieved by incorporating the concept of biorefinery, wherein acidogenic fermentation effluents were used for the production of PHA, which enabled the re-entry of products (VFA) to the production cycle, thus achieving circularity.
34134408	5	65	theme	α-cellulose/g	774:786	arg1	SCB					788:790	0.22 ± 0.02 g α-cellulose/g SCB	760:790	0.22 ± 0.02 g α-cellulose/g SCB	760:790	α-Cellulose (0.22 ± 0.02 g α-cellulose/g SCB) was extracted through SCB pretreatment.
34134408	5	65	theme	α-cellulose/g	774:786	arg1	α-Cellulose					747:757	α-Cellulose	747:757	α-Cellulose (0.22 ± 0.02 g α-cellulose/g SCB)	747:791	α-Cellulose (0.22 ± 0.02 g α-cellulose/g SCB) was extracted through SCB pretreatment.
34134408	7	66	theme	biocomposite	1041:1052	arg1	examination					1026:1036	FE-SEM morphological examination	1005:1036	FE-SEM morphological examination of biocomposite	1005:1052	FE-SEM morphological examination of biocomposite depicted that α-cellulose functioned as a filler to PHB.
34134408	4	67	theme	NMR	698:700	arg1	spectrum					702:709	NMR spectrum	698:709	NMR spectrum	698:709	Confocal examination showed the deposition of PHB granules in the cell cytoplasm and NMR spectrum exhibited a structural correlation.
34134408	0	68	with	grafting	77:84	arg1	bagasse					91:97	bagasse	91:97	bagasse	91:97	Polyhydroxybutyrate production from dark-fermentative effluent and composite grafting with bagasse derived α-cellulose in a biorefinery approach.
34134408	0	69	from	grafting	77:84	arg1	production					20:29	Polyhydroxybutyrate production	0:29	Polyhydroxybutyrate production from dark-fermentative effluent and composite grafting with bagasse	0:97	Polyhydroxybutyrate production from dark-fermentative effluent and composite grafting with bagasse derived α-cellulose in a biorefinery approach.
34134408	10	70	theme	PHB	1524:1526	arg1	expands					1528:1534	biologically produced PHB expands	1502:1534	biologically produced PHB expands	1502:1534	Utilization of bagasse-derived cellulose for strengthening biologically produced PHB expands its applications, while simultaneously addressing the plastic pollution issues.
34134408	7	71	theme	FE-SEM	1005:1010	arg1	examination					1026:1036	FE-SEM morphological examination	1005:1036	FE-SEM morphological examination of biocomposite	1005:1052	FE-SEM morphological examination of biocomposite depicted that α-cellulose functioned as a filler to PHB.
34134408	8	72	theme	significant	1131:1141	arg1	decrement					1143:1151	significant decrement	1131:1151	significant decrement	1131:1151	XRD profiles showed significant decrement in PHB crystallinity, signifying the functional role of α-cellulose as an effective reinforcing agent.
34134408	2	73	theme	sugarcane	458:466	arg1	SCB					477:479	SCB	477:479	SCB	477:479	PHB was produced using dark fermentation effluent rich in volatile fatty acids (VFA) derived from vegetable waste and α-cellulose was extracted from sugarcane bagasse (SCB).
34134408	2	73	theme	sugarcane	458:466	arg1	bagasse					468:474	sugarcane bagasse	458:474	sugarcane bagasse (SCB)	458:480	PHB was produced using dark fermentation effluent rich in volatile fatty acids (VFA) derived from vegetable waste and α-cellulose was extracted from sugarcane bagasse (SCB).
34134408	0	74	theme	dark-fermentative	36:52	arg1	effluent					54:61	dark-fermentative effluent	36:61	dark-fermentative effluent	36:61	Polyhydroxybutyrate production from dark-fermentative effluent and composite grafting with bagasse derived α-cellulose in a biorefinery approach.
34134408	9	75	theme	effective	1400:1408	arg1	blending					1410:1417	the effective blending	1396:1417	the effective blending of α-cellulose and PHB	1396:1440	Additionally, ether functional group of α-cellulose and ester group of PHB also appeared in XPS analysis of the composite, thus authorizing the effective blending of α-cellulose and PHB.
34134408	10	76	theme	pollution	1598:1606	arg1	issues					1608:1613	the plastic pollution issues	1586:1613	the plastic pollution issues	1586:1613	Utilization of bagasse-derived cellulose for strengthening biologically produced PHB expands its applications, while simultaneously addressing the plastic pollution issues.
34134408	11	77	theme	biorefinery	1704:1714	arg1	concept					1693:1699	the concept	1689:1699	the concept of biorefinery, wherein acidogenic fermentation effluents were used for the production of PHA, which enabled the re-entry of products (VFA) to the production cycle, thus achieving circularity	1689:1891	Additional value from this process was further achieved by incorporating the concept of biorefinery, wherein acidogenic fermentation effluents were used for the production of PHA, which enabled the re-entry of products (VFA) to the production cycle, thus achieving circularity.
34134408	5	78	theme	0.22 ± 0.02 g	760:772	arg1	SCB					788:790	0.22 ± 0.02 g α-cellulose/g SCB	760:790	0.22 ± 0.02 g α-cellulose/g SCB	760:790	α-Cellulose (0.22 ± 0.02 g α-cellulose/g SCB) was extracted through SCB pretreatment.
34134408	5	78	theme	0.22 ± 0.02 g	760:772	arg1	α-Cellulose					747:757	α-Cellulose	747:757	α-Cellulose (0.22 ± 0.02 g α-cellulose/g SCB)	747:791	α-Cellulose (0.22 ± 0.02 g α-cellulose/g SCB) was extracted through SCB pretreatment.
34134408	5	79	theme	SCB	815:817	arg1	pretreatment					819:830	SCB pretreatment	815:830	SCB pretreatment	815:830	α-Cellulose (0.22 ± 0.02 g α-cellulose/g SCB) was extracted through SCB pretreatment.
34134408	6	80	theme	intermolecular	883:896	arg1	bonding					898:904	intermolecular bonding	883:904	intermolecular bonding	883:904	Thereafter, grafting α-cellulose with PHB offered intermolecular bonding, which resulted in enhanced thermal stability of the biocomposite than corresponding pristine PHB.
34134408	3	81	theme	microbial	511:519	arg1	accumulation					525:536	microbial PHB accumulation	511:536	microbial PHB accumulation	511:536	Nutrient limitation induced microbial PHB accumulation, wherein maximum production of 0.28 ± 0.06 g PHB/g DCW (28%) was observed.
34134408	8	82	theme	functional	1190:1199	arg1	role					1201:1204	the functional role	1186:1204	the functional role of α-cellulose as an effective reinforcing agent	1186:1253	XRD profiles showed significant decrement in PHB crystallinity, signifying the functional role of α-cellulose as an effective reinforcing agent.
34134408	4	83	theme	Confocal	613:620	arg1	examination					622:632	Confocal examination	613:632	Confocal examination	613:632	Confocal examination showed the deposition of PHB granules in the cell cytoplasm and NMR spectrum exhibited a structural correlation.
34134408	11	84	theme	fermentation	1736:1747	arg1	effluents					1749:1757	acidogenic fermentation effluents	1725:1757	acidogenic fermentation effluents	1725:1757	Additional value from this process was further achieved by incorporating the concept of biorefinery, wherein acidogenic fermentation effluents were used for the production of PHA, which enabled the re-entry of products (VFA) to the production cycle, thus achieving circularity.
34134408	2	85	attach	derived	394:400	arg2	VFA					389:391	VFA	389:391	VFA	389:391	PHB was produced using dark fermentation effluent rich in volatile fatty acids (VFA) derived from vegetable waste and α-cellulose was extracted from sugarcane bagasse (SCB).
34134408	2	85	attach	derived	394:400	arg2	acids					382:386	volatile fatty acids	367:386	volatile fatty acids (VFA) derived from vegetable waste	367:421	PHB was produced using dark fermentation effluent rich in volatile fatty acids (VFA) derived from vegetable waste and α-cellulose was extracted from sugarcane bagasse (SCB).
34134408	2	85	attach	derived	394:400	arg1	waste					417:421	vegetable waste	407:421	vegetable waste	407:421	PHB was produced using dark fermentation effluent rich in volatile fatty acids (VFA) derived from vegetable waste and α-cellulose was extracted from sugarcane bagasse (SCB).
34134408	3	86	theme	wherein	539:545	arg1	production					555:564	wherein maximum production	539:564	wherein maximum production of 0.28 ± 0.06 g PHB/g DCW (28%)	539:597	Nutrient limitation induced microbial PHB accumulation, wherein maximum production of 0.28 ± 0.06 g PHB/g DCW (28%) was observed.
34115245	0	0	theme	nerve	96:100	arg1	material					109:116	a moldable nerve repair material	85:116	a moldable nerve repair material	85:116	Decellularized nerve extracellular matrix/chitosan crosslinked by genipin to prepare a moldable nerve repair material.
34115245	9	1	theme	glutaraldehyde	1464:1477	arg1	group					1479:1483	glutaraldehyde group	1464:1483	glutaraldehyde group	1464:1483	The histocompatibility of scaffold crosslinked by genipin was also better than glutaraldehyde group.
34115245	8	2	theme	Cell	1252:1255	arg1	cytotoxicity					1257:1268	Cell cytotoxicity	1252:1268	Cell cytotoxicity	1252:1268	Cell cytotoxicity showed that the cytotoxicity of scaffold crosslinked by genipin was lower than that crosslinked by glutaraldehyde.
34115245	0	3	theme	moldable	87:94	arg1	material					109:116	a moldable nerve repair material	85:116	a moldable nerve repair material	85:116	Decellularized nerve extracellular matrix/chitosan crosslinked by genipin to prepare a moldable nerve repair material.
34115245	4	4	theme	biocompatibility	551:566	arg1	of materials					568:579	Physicochemical property, degradation and biocompatibility of materials	509:579	Physicochemical property, degradation and biocompatibility of materials crosslinked by genipin, glutaraldehyde or ultraviolet ray	509:637	Physicochemical property, degradation and biocompatibility of materials crosslinked by genipin, glutaraldehyde or ultraviolet ray were evaluated.
34115245	10	5	theme	L929	1568:1571	arg1	cells					1573:1577	L929 cells	1568:1577	L929 cells	1568:1577	More cells grew well in the scaffold crosslinked by genipin when co-cultured with L929 cells.
34115245	11	6	theme	cord	1783:1786	arg1	engineering					1795:1805	the spinal cord tissue engineering	1772:1805	the spinal cord tissue engineering	1772:1805	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	7	7	theme	hydrolysis	1204:1213	arg1	ability					1215:1221	better water absorption, water retention and anti-enzymatic hydrolysis ability	1144:1221	better water absorption, water retention and anti-enzymatic hydrolysis ability	1144:1221	The scaffold crosslinked by genipin has better water absorption, water retention and anti-enzymatic hydrolysis ability than the other three groups.
34115245	2	8	theme	neural	309:314	arg1	reconstruction					323:336	neural tissue reconstruction	309:336	neural tissue reconstruction	309:336	But the rapid biodegradation of the materials may interrupt neural tissue reconstruction in vivo.
34115245	1	9	theme	Decellularized	119:132	arg1	matrix					154:159	Decellularized nerve extracellular matrix	119:159	Decellularized nerve extracellular matrix (NECM) composited with chitosan	119:191	Decellularized nerve extracellular matrix (NECM) composited with chitosan are moldable materials suitable for spinal cord repair.
34115245	1	9	theme	Decellularized	119:132	arg1	materials					206:214	moldable materials	197:214	moldable materials suitable for spinal cord repair	197:246	Decellularized nerve extracellular matrix (NECM) composited with chitosan are moldable materials suitable for spinal cord repair.
34115245	1	9	theme	Decellularized	119:132	arg1	NECM					162:165	NECM	162:165	NECM	162:165	Decellularized nerve extracellular matrix (NECM) composited with chitosan are moldable materials suitable for spinal cord repair.
34115245	5	10	contain	possessed	691:699	arg1	scaffold					659:666	The scaffold	655:666	The scaffold crosslinked by genipin	655:689	The scaffold crosslinked by genipin possessed a porous structure, and the porosity ratio was 89.07 + 4.90%, the average diameter of pore was 85.32 + 5.34 μm.
34115245	5	10	contain	possessed	691:699	arg2	structure					710:718	a porous structure	701:718	a porous structure	701:718	The scaffold crosslinked by genipin possessed a porous structure, and the porosity ratio was 89.07 + 4.90%, the average diameter of pore was 85.32 + 5.34 μm.
34115245	0	11	theme	repair	102:107	arg1	material					109:116	a moldable nerve repair material	85:116	a moldable nerve repair material	85:116	Decellularized nerve extracellular matrix/chitosan crosslinked by genipin to prepare a moldable nerve repair material.
34115245	11	12	theme	good	1675:1678	arg1	properties					1691:1700	good mechanical properties	1675:1700	good mechanical properties	1675:1700	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	11	12	theme	good	1675:1678	arg1	structure					1709:1717	micro structure	1703:1717	micro structure	1703:1717	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	11	12	theme	good	1675:1678	arg1	scaffold					1759:1766	an ideal scaffold	1750:1766	an ideal scaffold for the spinal cord tissue engineering	1750:1805	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	11	12	theme	good	1675:1678	arg1	biocompatibility					1723:1738	biocompatibility	1723:1738	biocompatibility	1723:1738	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	11	13	theme	spinal	1776:1781	arg1	cord					1783:1786	spinal cord	1776:1786	the spinal cord tissue engineering	1772:1805	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	5	14	theme	pore	787:790	arg1	diameter					775:782	the average diameter	763:782	the average diameter of pore	763:790	The scaffold crosslinked by genipin possessed a porous structure, and the porosity ratio was 89.07 + 4.90%, the average diameter of pore was 85.32 + 5.34 μm.
34115245	11	15	theme	mechanical	1680:1689	arg1	properties					1691:1700	good mechanical properties	1675:1700	good mechanical properties	1675:1700	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	11	15	theme	mechanical	1680:1689	arg1	structure					1709:1717	micro structure	1703:1717	micro structure	1703:1717	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	11	15	theme	mechanical	1680:1689	arg1	scaffold					1759:1766	an ideal scaffold	1750:1766	an ideal scaffold for the spinal cord tissue engineering	1750:1805	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	11	15	theme	mechanical	1680:1689	arg1	biocompatibility					1723:1738	biocompatibility	1723:1738	biocompatibility	1723:1738	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	11	16	theme	tissue	1788:1793	arg1	engineering					1795:1805	the spinal cord tissue engineering	1772:1805	the spinal cord tissue engineering	1772:1805	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	4	17	theme	Physicochemical property	509:532	arg1	of materials					568:579	Physicochemical property, degradation and biocompatibility of materials	509:579	Physicochemical property, degradation and biocompatibility of materials crosslinked by genipin, glutaraldehyde or ultraviolet ray	509:637	Physicochemical property, degradation and biocompatibility of materials crosslinked by genipin, glutaraldehyde or ultraviolet ray were evaluated.
34115245	3	18	dep	NECM	416:419	arg1	hydrogels					434:442	hydrogels	434:442	hydrogels	434:442	To improve the stability of the materials, the materials produced by NECM and chitosan hydrogels were crosslinked by genipine, glutaraldehyde or ultraviolet ray.
34115245	6	19	theme	scaffold	843:850	arg1	degree					829:834	The crosslinked degree	813:834	The crosslinked degree of the scaffold crosslinked by genipin and glutaraldehyde	813:892	The crosslinked degree of the scaffold crosslinked by genipin and glutaraldehyde was 75.13 ± 4.87%, 71.25 ± 5.06% respectively; Uncrosslinked scaffold disintegrated when immerged in distilled water while the scaffold crosslinked by genipin and glutaraldehyde group retained their integrity.
34115245	6	19	theme	scaffold	843:850	arg1	%					910:910	75.13 ± 4.87%	898:910	75.13 ± 4.87%	898:910	The crosslinked degree of the scaffold crosslinked by genipin and glutaraldehyde was 75.13 ± 4.87%, 71.25 ± 5.06% respectively; Uncrosslinked scaffold disintegrated when immerged in distilled water while the scaffold crosslinked by genipin and glutaraldehyde group retained their integrity.
34115245	2	20	theme	materials	285:293	arg1	biodegradation					263:276	the rapid biodegradation	253:276	the rapid biodegradation of the materials	253:293	But the rapid biodegradation of the materials may interrupt neural tissue reconstruction in vivo.
34115245	7	21	contain	has	1140:1142	arg2	ability					1215:1221	better water absorption, water retention and anti-enzymatic hydrolysis ability	1144:1221	better water absorption, water retention and anti-enzymatic hydrolysis ability	1144:1221	The scaffold crosslinked by genipin has better water absorption, water retention and anti-enzymatic hydrolysis ability than the other three groups.
34115245	7	21	contain	has	1140:1142	arg1	scaffold					1108:1115	The scaffold	1104:1115	The scaffold crosslinked by genipin	1104:1138	The scaffold crosslinked by genipin has better water absorption, water retention and anti-enzymatic hydrolysis ability than the other three groups.
34115245	0	22	theme	nerve	15:19	arg1	matrix/chitosan					35:49	Decellularized nerve extracellular matrix/chitosan	0:49	Decellularized nerve extracellular matrix/chitosan	0:49	Decellularized nerve extracellular matrix/chitosan crosslinked by genipin to prepare a moldable nerve repair material.
34115245	7	23	theme	water	1169:1173	arg1	retention					1175:1183	water retention	1169:1183	water retention	1169:1183	The scaffold crosslinked by genipin has better water absorption, water retention and anti-enzymatic hydrolysis ability than the other three groups.
34115245	1	24	theme	moldable	197:204	arg1	matrix					154:159	Decellularized nerve extracellular matrix	119:159	Decellularized nerve extracellular matrix (NECM) composited with chitosan	119:191	Decellularized nerve extracellular matrix (NECM) composited with chitosan are moldable materials suitable for spinal cord repair.
34115245	1	24	theme	moldable	197:204	arg1	materials					206:214	moldable materials	197:214	moldable materials suitable for spinal cord repair	197:246	Decellularized nerve extracellular matrix (NECM) composited with chitosan are moldable materials suitable for spinal cord repair.
34115245	0	25	theme	Decellularized	0:13	arg1	matrix/chitosan					35:49	Decellularized nerve extracellular matrix/chitosan	0:49	Decellularized nerve extracellular matrix/chitosan	0:49	Decellularized nerve extracellular matrix/chitosan crosslinked by genipin to prepare a moldable nerve repair material.
34115245	5	26	theme	porous	703:708	arg1	structure					710:718	a porous structure	701:718	a porous structure	701:718	The scaffold crosslinked by genipin possessed a porous structure, and the porosity ratio was 89.07 + 4.90%, the average diameter of pore was 85.32 + 5.34 μm.
34115245	11	27	theme	micro	1703:1707	arg1	properties					1691:1700	good mechanical properties	1675:1700	good mechanical properties	1675:1700	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	11	27	theme	micro	1703:1707	arg1	structure					1709:1717	micro structure	1703:1717	micro structure	1703:1717	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	11	28	theme	decellularized	1584:1597	arg1	scaffold					1635:1642	The decellularized nerve extracellular matrix/chitosan scaffold	1580:1642	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin	1580:1669	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	6	29	link	crosslinked	817:827	arg1	degree					829:834	The crosslinked degree	813:834	The crosslinked degree of the scaffold crosslinked by genipin and glutaraldehyde	813:892	The crosslinked degree of the scaffold crosslinked by genipin and glutaraldehyde was 75.13 ± 4.87%, 71.25 ± 5.06% respectively; Uncrosslinked scaffold disintegrated when immerged in distilled water while the scaffold crosslinked by genipin and glutaraldehyde group retained their integrity.
34115245	6	29	link	crosslinked	817:827	arg1	%					910:910	75.13 ± 4.87%	898:910	75.13 ± 4.87%	898:910	The crosslinked degree of the scaffold crosslinked by genipin and glutaraldehyde was 75.13 ± 4.87%, 71.25 ± 5.06% respectively; Uncrosslinked scaffold disintegrated when immerged in distilled water while the scaffold crosslinked by genipin and glutaraldehyde group retained their integrity.
34115245	7	30	theme	absorption	1157:1166	arg1	ability					1215:1221	better water absorption, water retention and anti-enzymatic hydrolysis ability	1144:1221	better water absorption, water retention and anti-enzymatic hydrolysis ability	1144:1221	The scaffold crosslinked by genipin has better water absorption, water retention and anti-enzymatic hydrolysis ability than the other three groups.
34115245	11	31	theme	nerve	1599:1603	arg1	scaffold					1635:1642	The decellularized nerve extracellular matrix/chitosan scaffold	1580:1642	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin	1580:1669	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	1	32	theme	suitable	216:223	arg1	matrix					154:159	Decellularized nerve extracellular matrix	119:159	Decellularized nerve extracellular matrix (NECM) composited with chitosan	119:191	Decellularized nerve extracellular matrix (NECM) composited with chitosan are moldable materials suitable for spinal cord repair.
34115245	1	32	theme	suitable	216:223	arg1	materials					206:214	moldable materials	197:214	moldable materials suitable for spinal cord repair	197:246	Decellularized nerve extracellular matrix (NECM) composited with chitosan are moldable materials suitable for spinal cord repair.
34115245	0	33	theme	extracellular	21:33	arg1	matrix/chitosan					35:49	Decellularized nerve extracellular matrix/chitosan	0:49	Decellularized nerve extracellular matrix/chitosan	0:49	Decellularized nerve extracellular matrix/chitosan crosslinked by genipin to prepare a moldable nerve repair material.
34115245	4	34	theme	ultraviolet	623:633	arg1	ray					635:637	ultraviolet ray	623:637	ultraviolet ray	623:637	Physicochemical property, degradation and biocompatibility of materials crosslinked by genipin, glutaraldehyde or ultraviolet ray were evaluated.
34115245	2	35	theme	tissue	316:321	arg1	reconstruction					323:336	neural tissue reconstruction	309:336	neural tissue reconstruction	309:336	But the rapid biodegradation of the materials may interrupt neural tissue reconstruction in vivo.
34115245	7	36	theme	better	1144:1149	arg1	ability					1215:1221	better water absorption, water retention and anti-enzymatic hydrolysis ability	1144:1221	better water absorption, water retention and anti-enzymatic hydrolysis ability	1144:1221	The scaffold crosslinked by genipin has better water absorption, water retention and anti-enzymatic hydrolysis ability than the other three groups.
34115245	11	37	theme	matrix/chitosan	1619:1633	arg1	scaffold					1635:1642	The decellularized nerve extracellular matrix/chitosan scaffold	1580:1642	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin	1580:1669	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	1	38	theme	spinal	229:234	arg1	repair					241:246	spinal cord repair	229:246	spinal cord repair	229:246	Decellularized nerve extracellular matrix (NECM) composited with chitosan are moldable materials suitable for spinal cord repair.
34115245	5	39	theme	average	767:773	arg1	diameter					775:782	the average diameter	763:782	the average diameter of pore	763:790	The scaffold crosslinked by genipin possessed a porous structure, and the porosity ratio was 89.07 + 4.90%, the average diameter of pore was 85.32 + 5.34 μm.
34115245	11	40	contain	has	1671:1673	arg1	scaffold					1635:1642	The decellularized nerve extracellular matrix/chitosan scaffold	1580:1642	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin	1580:1669	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	11	40	contain	has	1671:1673	arg2	biocompatibility					1723:1738	biocompatibility	1723:1738	biocompatibility	1723:1738	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	11	40	contain	has	1671:1673	arg2	structure					1709:1717	micro structure	1703:1717	micro structure	1703:1717	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	11	40	contain	has	1671:1673	arg2	scaffold					1759:1766	an ideal scaffold	1750:1766	an ideal scaffold for the spinal cord tissue engineering	1750:1805	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	11	40	contain	has	1671:1673	arg2	properties					1691:1700	good mechanical properties	1675:1700	good mechanical properties	1675:1700	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	8	41	theme	scaffold	1302:1309	arg1	lower					1338:1342	lower	1338:1342	lower	1338:1342	Cell cytotoxicity showed that the cytotoxicity of scaffold crosslinked by genipin was lower than that crosslinked by glutaraldehyde.
34115245	8	41	theme	scaffold	1302:1309	arg1	cytotoxicity					1286:1297	the cytotoxicity	1282:1297	the cytotoxicity of scaffold crosslinked by genipin	1282:1332	Cell cytotoxicity showed that the cytotoxicity of scaffold crosslinked by genipin was lower than that crosslinked by glutaraldehyde.
34115245	6	42	theme	crosslinked	817:827	arg1	degree					829:834	The crosslinked degree	813:834	The crosslinked degree of the scaffold crosslinked by genipin and glutaraldehyde	813:892	The crosslinked degree of the scaffold crosslinked by genipin and glutaraldehyde was 75.13 ± 4.87%, 71.25 ± 5.06% respectively; Uncrosslinked scaffold disintegrated when immerged in distilled water while the scaffold crosslinked by genipin and glutaraldehyde group retained their integrity.
34115245	6	42	theme	crosslinked	817:827	arg1	%					910:910	75.13 ± 4.87%	898:910	75.13 ± 4.87%	898:910	The crosslinked degree of the scaffold crosslinked by genipin and glutaraldehyde was 75.13 ± 4.87%, 71.25 ± 5.06% respectively; Uncrosslinked scaffold disintegrated when immerged in distilled water while the scaffold crosslinked by genipin and glutaraldehyde group retained their integrity.
34115245	1	43	theme	cord	236:239	arg1	repair					241:246	spinal cord repair	229:246	spinal cord repair	229:246	Decellularized nerve extracellular matrix (NECM) composited with chitosan are moldable materials suitable for spinal cord repair.
34115245	7	44	theme	other	1232:1236	arg1	groups					1244:1249	the other three groups	1228:1249	the other three groups	1228:1249	The scaffold crosslinked by genipin has better water absorption, water retention and anti-enzymatic hydrolysis ability than the other three groups.
34115245	5	45	theme	porosity	729:736	arg1	ratio					738:742	the porosity ratio	725:742	the porosity ratio	725:742	The scaffold crosslinked by genipin possessed a porous structure, and the porosity ratio was 89.07 + 4.90%, the average diameter of pore was 85.32 + 5.34 μm.
34115245	5	45	theme	porosity	729:736	arg1	%					760:760	89.07 + 4.90%	748:760	89.07 + 4.90%	748:760	The scaffold crosslinked by genipin possessed a porous structure, and the porosity ratio was 89.07 + 4.90%, the average diameter of pore was 85.32 + 5.34 μm.
34115245	6	46	theme	Uncrosslinked	941:953	arg1	scaffold					955:962	Uncrosslinked scaffold	941:962	Uncrosslinked scaffold	941:962	The crosslinked degree of the scaffold crosslinked by genipin and glutaraldehyde was 75.13 ± 4.87%, 71.25 ± 5.06% respectively; Uncrosslinked scaffold disintegrated when immerged in distilled water while the scaffold crosslinked by genipin and glutaraldehyde group retained their integrity.
34115245	1	47	theme	nerve	134:138	arg1	matrix					154:159	Decellularized nerve extracellular matrix	119:159	Decellularized nerve extracellular matrix (NECM) composited with chitosan	119:191	Decellularized nerve extracellular matrix (NECM) composited with chitosan are moldable materials suitable for spinal cord repair.
34115245	1	47	theme	nerve	134:138	arg1	materials					206:214	moldable materials	197:214	moldable materials suitable for spinal cord repair	197:246	Decellularized nerve extracellular matrix (NECM) composited with chitosan are moldable materials suitable for spinal cord repair.
34115245	1	47	theme	nerve	134:138	arg1	NECM					162:165	NECM	162:165	NECM	162:165	Decellularized nerve extracellular matrix (NECM) composited with chitosan are moldable materials suitable for spinal cord repair.
34115245	7	48	theme	water	1151:1155	arg1	absorption					1157:1166	water absorption	1151:1166	water absorption	1151:1166	The scaffold crosslinked by genipin has better water absorption, water retention and anti-enzymatic hydrolysis ability than the other three groups.
34115245	6	49	theme	distilled	995:1003	arg1	water					1005:1009	distilled water	995:1009	distilled water	995:1009	The crosslinked degree of the scaffold crosslinked by genipin and glutaraldehyde was 75.13 ± 4.87%, 71.25 ± 5.06% respectively; Uncrosslinked scaffold disintegrated when immerged in distilled water while the scaffold crosslinked by genipin and glutaraldehyde group retained their integrity.
34115245	7	50	theme	anti-enzymatic	1189:1202	arg1	hydrolysis					1204:1213	anti-enzymatic hydrolysis	1189:1213	anti-enzymatic hydrolysis	1189:1213	The scaffold crosslinked by genipin has better water absorption, water retention and anti-enzymatic hydrolysis ability than the other three groups.
34115245	1	51	theme	extracellular	140:152	arg1	matrix					154:159	Decellularized nerve extracellular matrix	119:159	Decellularized nerve extracellular matrix (NECM) composited with chitosan	119:191	Decellularized nerve extracellular matrix (NECM) composited with chitosan are moldable materials suitable for spinal cord repair.
34115245	1	51	theme	extracellular	140:152	arg1	materials					206:214	moldable materials	197:214	moldable materials suitable for spinal cord repair	197:246	Decellularized nerve extracellular matrix (NECM) composited with chitosan are moldable materials suitable for spinal cord repair.
34115245	1	51	theme	extracellular	140:152	arg1	NECM					162:165	NECM	162:165	NECM	162:165	Decellularized nerve extracellular matrix (NECM) composited with chitosan are moldable materials suitable for spinal cord repair.
34115245	3	52	theme	ultraviolet	492:502	arg1	ray					504:506	ultraviolet ray	492:506	ultraviolet ray	492:506	To improve the stability of the materials, the materials produced by NECM and chitosan hydrogels were crosslinked by genipine, glutaraldehyde or ultraviolet ray.
34115245	11	53	theme	ideal	1753:1757	arg1	properties					1691:1700	good mechanical properties	1675:1700	good mechanical properties	1675:1700	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	11	53	theme	ideal	1753:1757	arg1	scaffold					1759:1766	an ideal scaffold	1750:1766	an ideal scaffold for the spinal cord tissue engineering	1750:1805	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	11	54	theme	extracellular	1605:1617	arg1	scaffold					1635:1642	The decellularized nerve extracellular matrix/chitosan scaffold	1580:1642	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin	1580:1669	The decellularized nerve extracellular matrix/chitosan scaffold crosslinked by the genipin has good mechanical properties, micro structure and biocompatibility, which is an ideal scaffold for the spinal cord tissue engineering.
34115245	7	55	theme	retention	1175:1183	arg1	ability					1215:1221	better water absorption, water retention and anti-enzymatic hydrolysis ability	1144:1221	better water absorption, water retention and anti-enzymatic hydrolysis ability	1144:1221	The scaffold crosslinked by genipin has better water absorption, water retention and anti-enzymatic hydrolysis ability than the other three groups.
34115245	6	56	dep	genipin	1045:1051	arg1	group					1072:1076	group	1072:1076	group	1072:1076	The crosslinked degree of the scaffold crosslinked by genipin and glutaraldehyde was 75.13 ± 4.87%, 71.25 ± 5.06% respectively; Uncrosslinked scaffold disintegrated when immerged in distilled water while the scaffold crosslinked by genipin and glutaraldehyde group retained their integrity.
34115245	6	57	link	Uncrosslinked	941:953	arg1	scaffold					955:962	Uncrosslinked scaffold	941:962	Uncrosslinked scaffold	941:962	The crosslinked degree of the scaffold crosslinked by genipin and glutaraldehyde was 75.13 ± 4.87%, 71.25 ± 5.06% respectively; Uncrosslinked scaffold disintegrated when immerged in distilled water while the scaffold crosslinked by genipin and glutaraldehyde group retained their integrity.
34115245	9	58	theme	scaffold	1411:1418	arg1	histocompatibility					1389:1406	The histocompatibility	1385:1406	The histocompatibility of scaffold crosslinked by genipin	1385:1441	The histocompatibility of scaffold crosslinked by genipin was also better than glutaraldehyde group.
34115245	9	58	theme	scaffold	1411:1418	arg1	better					1452:1457	better	1452:1457	better	1452:1457	The histocompatibility of scaffold crosslinked by genipin was also better than glutaraldehyde group.
34115245	4	59	theme	degradation	535:545	arg1	of materials					568:579	Physicochemical property, degradation and biocompatibility of materials	509:579	Physicochemical property, degradation and biocompatibility of materials crosslinked by genipin, glutaraldehyde or ultraviolet ray	509:637	Physicochemical property, degradation and biocompatibility of materials crosslinked by genipin, glutaraldehyde or ultraviolet ray were evaluated.
34115245	2	60	theme	rapid	257:261	arg1	biodegradation					263:276	the rapid biodegradation	253:276	the rapid biodegradation of the materials	253:293	But the rapid biodegradation of the materials may interrupt neural tissue reconstruction in vivo.
34115245	10	61	theme	More	1486:1489	arg1	cells					1491:1495	More cells	1486:1495	More cells	1486:1495	More cells grew well in the scaffold crosslinked by genipin when co-cultured with L929 cells.
34115245	3	62	theme	materials	379:387	arg1	stability					362:370	the stability	358:370	the stability of the materials	358:387	To improve the stability of the materials, the materials produced by NECM and chitosan hydrogels were crosslinked by genipine, glutaraldehyde or ultraviolet ray.
34848084	0	0	theme	antioxidant	83:93	arg1	nanocomposites					95:108	quantum dot antioxidant nanocomposites	71:108	quantum dot antioxidant nanocomposites	71:108	Preparation, characterization of naringenin, β-cyclodextrin and carbon quantum dot antioxidant nanocomposites.
34848084	1	1	theme	carbon	162:167	arg1	dots					177:180	carbon quantum dots	162:180	carbon quantum dots	162:180	In this work, naringenin loaded β-cyclodextrin and carbon quantum dots composite nanoparticles were successfully fabricated.
34848084	5	2	theme	naringenin	854:863	arg1	state					845:849	the crystalline state	829:849	the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex	829:907	X-Ray Diffraction experiment revealed that the material state of the formed naringenin-β-CD-CQDs nanoparticles is amorphous in opposition to the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex.
34848084	0	3	theme	dot	79:81	arg1	nanocomposites					95:108	quantum dot antioxidant nanocomposites	71:108	quantum dot antioxidant nanocomposites	71:108	Preparation, characterization of naringenin, β-cyclodextrin and carbon quantum dot antioxidant nanocomposites.
34848084	6	4	theme	formed	1036:1041	arg1	nanoparticles					1053:1065	the formed composite nanoparticles	1032:1065	the formed composite nanoparticles	1032:1065	Finally, antioxidant activity analyses against DPPH, ABTS+ and Fe2+ chelating, showed an enhanced antioxidant activity of the formed composite nanoparticles compared to their constituents.
34848084	7	5	theme	composite	1219:1227	arg1	nanoparticles					1229:1241	composite nanoparticles	1219:1241	composite nanoparticles	1219:1241	These results indicated that naringenin can be effectively entrapped in β-cyclodextrin and carbon quantum dots, forming composite nanoparticles with improved antioxidant properties.
34848084	1	6	theme	quantum	169:175	arg1	dots					177:180	carbon quantum dots	162:180	carbon quantum dots	162:180	In this work, naringenin loaded β-cyclodextrin and carbon quantum dots composite nanoparticles were successfully fabricated.
34848084	5	7	theme	crystalline	833:843	arg1	state					845:849	the crystalline state	829:849	the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex	829:907	X-Ray Diffraction experiment revealed that the material state of the formed naringenin-β-CD-CQDs nanoparticles is amorphous in opposition to the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex.
34848084	7	8	theme	antioxidant	1257:1267	arg1	properties					1269:1278	improved antioxidant properties	1248:1278	improved antioxidant properties	1248:1278	These results indicated that naringenin can be effectively entrapped in β-cyclodextrin and carbon quantum dots, forming composite nanoparticles with improved antioxidant properties.
34848084	5	9	theme	X-Ray	688:692	arg1	Diffraction					694:704	X-Ray Diffraction	688:704	X-Ray Diffraction experiment	688:715	X-Ray Diffraction experiment revealed that the material state of the formed naringenin-β-CD-CQDs nanoparticles is amorphous in opposition to the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex.
34848084	2	10	theme	antioxidant	315:325	arg1	activities					327:336	antioxidant activities	315:336	antioxidant activities of nanoparticles	315:353	The results showed that incorporation of carbon quantum dots not only enhanced antioxidant activities of nanoparticles but also improved encapsulation efficiency of naringenin.
34848084	6	11	theme	antioxidant	1008:1018	arg1	activity					1020:1027	an enhanced antioxidant activity	996:1027	an enhanced antioxidant activity of the formed composite nanoparticles	996:1065	Finally, antioxidant activity analyses against DPPH, ABTS+ and Fe2+ chelating, showed an enhanced antioxidant activity of the formed composite nanoparticles compared to their constituents.
34848084	1	12	dep	loaded	136:141	arg1	naringenin					125:134	naringenin	125:134	naringenin	125:134	In this work, naringenin loaded β-cyclodextrin and carbon quantum dots composite nanoparticles were successfully fabricated.
34848084	5	13	theme	Diffraction	694:704	arg1	experiment					706:715	X-Ray Diffraction experiment	688:715	X-Ray Diffraction experiment	688:715	X-Ray Diffraction experiment revealed that the material state of the formed naringenin-β-CD-CQDs nanoparticles is amorphous in opposition to the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex.
34848084	2	14	theme	naringenin	401:410	arg1	efficiency					387:396	encapsulation efficiency	373:396	encapsulation efficiency of naringenin	373:410	The results showed that incorporation of carbon quantum dots not only enhanced antioxidant activities of nanoparticles but also improved encapsulation efficiency of naringenin.
34848084	5	15	from	opposition	815:824	arg1	amorphous					802:810	amorphous	802:810	amorphous	802:810	X-Ray Diffraction experiment revealed that the material state of the formed naringenin-β-CD-CQDs nanoparticles is amorphous in opposition to the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex.
34848084	5	15	from	opposition	815:824	arg1	state					744:748	the material state	731:748	the material state of the formed naringenin-β-CD-CQDs nanoparticles	731:797	X-Ray Diffraction experiment revealed that the material state of the formed naringenin-β-CD-CQDs nanoparticles is amorphous in opposition to the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex.
34848084	6	16	theme	enhanced	999:1006	arg1	activity					1020:1027	an enhanced antioxidant activity	996:1027	an enhanced antioxidant activity of the formed composite nanoparticles	996:1065	Finally, antioxidant activity analyses against DPPH, ABTS+ and Fe2+ chelating, showed an enhanced antioxidant activity of the formed composite nanoparticles compared to their constituents.
34848084	1	17	theme	dots	177:180	arg1	nanoparticles					192:204	naringenin loaded β-cyclodextrin and carbon quantum dots composite nanoparticles	125:204	naringenin loaded β-cyclodextrin and carbon quantum dots composite nanoparticles	125:204	In this work, naringenin loaded β-cyclodextrin and carbon quantum dots composite nanoparticles were successfully fabricated.
34848084	3	18	theme	nanoparticles	449:461	arg1	formation					426:434	the formation	422:434	the formation of composite nanoparticles	422:461	Further, the formation of composite nanoparticles was confirmed by a series of characterization methods.
34848084	7	19	theme	improved	1248:1255	arg1	properties					1269:1278	improved antioxidant properties	1248:1278	improved antioxidant properties	1248:1278	These results indicated that naringenin can be effectively entrapped in β-cyclodextrin and carbon quantum dots, forming composite nanoparticles with improved antioxidant properties.
34848084	1	20	theme	composite	182:190	arg1	nanoparticles					192:204	naringenin loaded β-cyclodextrin and carbon quantum dots composite nanoparticles	125:204	naringenin loaded β-cyclodextrin and carbon quantum dots composite nanoparticles	125:204	In this work, naringenin loaded β-cyclodextrin and carbon quantum dots composite nanoparticles were successfully fabricated.
34848084	5	21	theme	naringenin-β-CD	875:889	arg1	state					845:849	the crystalline state	829:849	the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex	829:907	X-Ray Diffraction experiment revealed that the material state of the formed naringenin-β-CD-CQDs nanoparticles is amorphous in opposition to the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex.
34848084	7	22	theme	carbon	1190:1195	arg1	dots					1205:1208	carbon quantum dots	1190:1208	carbon quantum dots	1190:1208	These results indicated that naringenin can be effectively entrapped in β-cyclodextrin and carbon quantum dots, forming composite nanoparticles with improved antioxidant properties.
34848084	6	23	dep	DPPH	957:960	arg1	chelating					978:986	chelating	978:986	chelating	978:986	Finally, antioxidant activity analyses against DPPH, ABTS+ and Fe2+ chelating, showed an enhanced antioxidant activity of the formed composite nanoparticles compared to their constituents.
34848084	5	24	dep	naringenin	854:863	arg1	complex					901:907	inclusion complex	891:907	inclusion complex	891:907	X-Ray Diffraction experiment revealed that the material state of the formed naringenin-β-CD-CQDs nanoparticles is amorphous in opposition to the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex.
34848084	4	25	dep	transform	549:557	arg1	infrared					559:566	infrared	559:566	transform infrared spectroscopy data	549:584	The zeta-potential and Fourier transform infrared spectroscopy data proved that electrostatic interaction and hydrogen bonding are dominant forces to form nanoparticles.
34848084	5	26	theme	material	735:742	arg1	amorphous					802:810	amorphous	802:810	amorphous	802:810	X-Ray Diffraction experiment revealed that the material state of the formed naringenin-β-CD-CQDs nanoparticles is amorphous in opposition to the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex.
34848084	5	26	theme	material	735:742	arg1	state					744:748	the material state	731:748	the material state of the formed naringenin-β-CD-CQDs nanoparticles	731:797	X-Ray Diffraction experiment revealed that the material state of the formed naringenin-β-CD-CQDs nanoparticles is amorphous in opposition to the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex.
34848084	2	27	theme	nanoparticles	341:353	arg1	activities					327:336	antioxidant activities	315:336	antioxidant activities of nanoparticles	315:353	The results showed that incorporation of carbon quantum dots not only enhanced antioxidant activities of nanoparticles but also improved encapsulation efficiency of naringenin.
34848084	4	28	theme	hydrogen	628:635	arg1	interaction					612:622	electrostatic interaction	598:622	electrostatic interaction	598:622	The zeta-potential and Fourier transform infrared spectroscopy data proved that electrostatic interaction and hydrogen bonding are dominant forces to form nanoparticles.
34848084	4	28	theme	hydrogen	628:635	arg1	bonding					637:643	hydrogen bonding	628:643	hydrogen bonding	628:643	The zeta-potential and Fourier transform infrared spectroscopy data proved that electrostatic interaction and hydrogen bonding are dominant forces to form nanoparticles.
34848084	4	28	theme	hydrogen	628:635	arg1	forces					658:663	dominant forces	649:663	dominant forces	649:663	The zeta-potential and Fourier transform infrared spectroscopy data proved that electrostatic interaction and hydrogen bonding are dominant forces to form nanoparticles.
34848084	4	29	theme	dominant	649:656	arg1	interaction					612:622	electrostatic interaction	598:622	electrostatic interaction	598:622	The zeta-potential and Fourier transform infrared spectroscopy data proved that electrostatic interaction and hydrogen bonding are dominant forces to form nanoparticles.
34848084	4	29	theme	dominant	649:656	arg1	bonding					637:643	hydrogen bonding	628:643	hydrogen bonding	628:643	The zeta-potential and Fourier transform infrared spectroscopy data proved that electrostatic interaction and hydrogen bonding are dominant forces to form nanoparticles.
34848084	4	29	theme	dominant	649:656	arg1	forces					658:663	dominant forces	649:663	dominant forces	649:663	The zeta-potential and Fourier transform infrared spectroscopy data proved that electrostatic interaction and hydrogen bonding are dominant forces to form nanoparticles.
34848084	6	30	theme	antioxidant	919:929	arg1	analyses					940:947	antioxidant activity analyses	919:947	antioxidant activity analyses	919:947	Finally, antioxidant activity analyses against DPPH, ABTS+ and Fe2+ chelating, showed an enhanced antioxidant activity of the formed composite nanoparticles compared to their constituents.
34848084	5	31	theme	inclusion	891:899	arg1	complex					901:907	inclusion complex	891:907	inclusion complex	891:907	X-Ray Diffraction experiment revealed that the material state of the formed naringenin-β-CD-CQDs nanoparticles is amorphous in opposition to the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex.
34848084	4	32	dep	zeta-potential	522:535	arg1	transform					549:557	transform	549:557	transform infrared spectroscopy data	549:584	The zeta-potential and Fourier transform infrared spectroscopy data proved that electrostatic interaction and hydrogen bonding are dominant forces to form nanoparticles.
34848084	7	33	theme	quantum	1197:1203	arg1	dots					1205:1208	carbon quantum dots	1190:1208	carbon quantum dots	1190:1208	These results indicated that naringenin can be effectively entrapped in β-cyclodextrin and carbon quantum dots, forming composite nanoparticles with improved antioxidant properties.
34848084	3	34	theme	characterization	492:507	arg1	methods					509:515	characterization methods	492:515	characterization methods	492:515	Further, the formation of composite nanoparticles was confirmed by a series of characterization methods.
34848084	0	35	theme	naringenin	33:42	arg1	characterization					13:28	characterization	13:28	characterization of naringenin, β-cyclodextrin and carbon quantum dot antioxidant nanocomposites	13:108	Preparation, characterization of naringenin, β-cyclodextrin and carbon quantum dot antioxidant nanocomposites.
34848084	0	35	theme	naringenin	33:42	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, characterization of naringenin, β-cyclodextrin and carbon quantum dot antioxidant nanocomposites.
34848084	4	36	theme	electrostatic	598:610	arg1	interaction					612:622	electrostatic interaction	598:622	electrostatic interaction	598:622	The zeta-potential and Fourier transform infrared spectroscopy data proved that electrostatic interaction and hydrogen bonding are dominant forces to form nanoparticles.
34848084	4	36	theme	electrostatic	598:610	arg1	bonding					637:643	hydrogen bonding	628:643	hydrogen bonding	628:643	The zeta-potential and Fourier transform infrared spectroscopy data proved that electrostatic interaction and hydrogen bonding are dominant forces to form nanoparticles.
34848084	4	36	theme	electrostatic	598:610	arg1	forces					658:663	dominant forces	649:663	dominant forces	649:663	The zeta-potential and Fourier transform infrared spectroscopy data proved that electrostatic interaction and hydrogen bonding are dominant forces to form nanoparticles.
34848084	3	37	theme	methods	509:515	arg1	series					482:487	a series	480:487	a series of characterization methods	480:515	Further, the formation of composite nanoparticles was confirmed by a series of characterization methods.
34848084	3	38	theme	composite	439:447	arg1	nanoparticles					449:461	composite nanoparticles	439:461	composite nanoparticles	439:461	Further, the formation of composite nanoparticles was confirmed by a series of characterization methods.
34848084	5	39	theme	naringenin-β-CD-CQDs	764:783	arg1	nanoparticles					785:797	the formed naringenin-β-CD-CQDs nanoparticles	753:797	the formed naringenin-β-CD-CQDs nanoparticles	753:797	X-Ray Diffraction experiment revealed that the material state of the formed naringenin-β-CD-CQDs nanoparticles is amorphous in opposition to the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex.
34848084	5	40	theme	β-CD	866:869	arg1	state					845:849	the crystalline state	829:849	the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex	829:907	X-Ray Diffraction experiment revealed that the material state of the formed naringenin-β-CD-CQDs nanoparticles is amorphous in opposition to the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex.
34848084	0	41	theme	β-cyclodextrin	45:58	arg1	characterization					13:28	characterization	13:28	characterization of naringenin, β-cyclodextrin and carbon quantum dot antioxidant nanocomposites	13:108	Preparation, characterization of naringenin, β-cyclodextrin and carbon quantum dot antioxidant nanocomposites.
34848084	0	41	theme	β-cyclodextrin	45:58	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, characterization of naringenin, β-cyclodextrin and carbon quantum dot antioxidant nanocomposites.
34848084	6	42	theme	nanoparticles	1053:1065	arg1	activity					1020:1027	an enhanced antioxidant activity	996:1027	an enhanced antioxidant activity of the formed composite nanoparticles	996:1065	Finally, antioxidant activity analyses against DPPH, ABTS+ and Fe2+ chelating, showed an enhanced antioxidant activity of the formed composite nanoparticles compared to their constituents.
34848084	5	43	theme	nanoparticles	785:797	arg1	amorphous					802:810	amorphous	802:810	amorphous	802:810	X-Ray Diffraction experiment revealed that the material state of the formed naringenin-β-CD-CQDs nanoparticles is amorphous in opposition to the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex.
34848084	5	43	theme	nanoparticles	785:797	arg1	state					744:748	the material state	731:748	the material state of the formed naringenin-β-CD-CQDs nanoparticles	731:797	X-Ray Diffraction experiment revealed that the material state of the formed naringenin-β-CD-CQDs nanoparticles is amorphous in opposition to the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex.
34848084	0	44	dep	naringenin	33:42	arg1	nanocomposites					95:108	quantum dot antioxidant nanocomposites	71:108	quantum dot antioxidant nanocomposites	71:108	Preparation, characterization of naringenin, β-cyclodextrin and carbon quantum dot antioxidant nanocomposites.
34848084	2	45	theme	encapsulation	373:385	arg1	efficiency					387:396	encapsulation efficiency	373:396	encapsulation efficiency of naringenin	373:410	The results showed that incorporation of carbon quantum dots not only enhanced antioxidant activities of nanoparticles but also improved encapsulation efficiency of naringenin.
34848084	6	46	theme	composite	1043:1051	arg1	nanoparticles					1053:1065	the formed composite nanoparticles	1032:1065	the formed composite nanoparticles	1032:1065	Finally, antioxidant activity analyses against DPPH, ABTS+ and Fe2+ chelating, showed an enhanced antioxidant activity of the formed composite nanoparticles compared to their constituents.
34848084	2	47	theme	dots	292:295	arg1	incorporation					260:272	incorporation	260:272	incorporation of carbon quantum dots	260:295	The results showed that incorporation of carbon quantum dots not only enhanced antioxidant activities of nanoparticles but also improved encapsulation efficiency of naringenin.
34848084	6	48	theme	activity	931:938	arg1	analyses					940:947	antioxidant activity analyses	919:947	antioxidant activity analyses	919:947	Finally, antioxidant activity analyses against DPPH, ABTS+ and Fe2+ chelating, showed an enhanced antioxidant activity of the formed composite nanoparticles compared to their constituents.
34848084	2	49	theme	quantum	284:290	arg1	dots					292:295	carbon quantum dots	277:295	carbon quantum dots	277:295	The results showed that incorporation of carbon quantum dots not only enhanced antioxidant activities of nanoparticles but also improved encapsulation efficiency of naringenin.
34848084	1	50	theme	loaded	136:141	arg1	β-cyclodextrin					143:156	naringenin loaded β-cyclodextrin	125:156	naringenin loaded β-cyclodextrin	125:156	In this work, naringenin loaded β-cyclodextrin and carbon quantum dots composite nanoparticles were successfully fabricated.
34848084	5	51	from	amorphous	802:810	arg1	opposition					815:824	opposition	815:824	opposition to the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex	815:907	X-Ray Diffraction experiment revealed that the material state of the formed naringenin-β-CD-CQDs nanoparticles is amorphous in opposition to the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex.
34848084	0	52	theme	quantum	71:77	arg1	nanocomposites					95:108	quantum dot antioxidant nanocomposites	71:108	quantum dot antioxidant nanocomposites	71:108	Preparation, characterization of naringenin, β-cyclodextrin and carbon quantum dot antioxidant nanocomposites.
34848084	2	53	theme	carbon	277:282	arg1	dots					292:295	carbon quantum dots	277:295	carbon quantum dots	277:295	The results showed that incorporation of carbon quantum dots not only enhanced antioxidant activities of nanoparticles but also improved encapsulation efficiency of naringenin.
34848084	1	54	theme	β-cyclodextrin	143:156	arg1	nanoparticles					192:204	naringenin loaded β-cyclodextrin and carbon quantum dots composite nanoparticles	125:204	naringenin loaded β-cyclodextrin and carbon quantum dots composite nanoparticles	125:204	In this work, naringenin loaded β-cyclodextrin and carbon quantum dots composite nanoparticles were successfully fabricated.
34848084	5	55	theme	formed	757:762	arg1	nanoparticles					785:797	the formed naringenin-β-CD-CQDs nanoparticles	753:797	the formed naringenin-β-CD-CQDs nanoparticles	753:797	X-Ray Diffraction experiment revealed that the material state of the formed naringenin-β-CD-CQDs nanoparticles is amorphous in opposition to the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex.
34848084	0	56	theme	carbon	64:69	arg1	characterization					13:28	characterization	13:28	characterization of naringenin, β-cyclodextrin and carbon quantum dot antioxidant nanocomposites	13:108	Preparation, characterization of naringenin, β-cyclodextrin and carbon quantum dot antioxidant nanocomposites.
34848084	0	56	theme	carbon	64:69	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, characterization of naringenin, β-cyclodextrin and carbon quantum dot antioxidant nanocomposites.
34848084	4	57	theme	spectroscopy	568:579	arg1	data					581:584	spectroscopy data	568:584	spectroscopy data	568:584	The zeta-potential and Fourier transform infrared spectroscopy data proved that electrostatic interaction and hydrogen bonding are dominant forces to form nanoparticles.
33965104	0	0	theme	self-healing	97:108	arg1	hydrogel					110:117	conductive self-healing hydrogel	86:117	conductive self-healing hydrogel for sensor	86:128	Synergy coordination of cellulose-based dialdehyde and carboxyl with Fe3+ recoverable conductive self-healing hydrogel for sensor.
33965104	3	1	theme	80	720:721	arg1	%					722:722	%	722:722	%	722:722	The elongation (~1300%) of the hydrogel containing 1 wt% DACNFs was approximate 13-fold of the pure PAA hydrogel and can recover to original state within 2 min after 80% compression.
33965104	1	2	theme	composite	174:182	arg1	hydrogel					208:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel	131:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel	131:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel was fabricated with high elongation, rapid recoverability, high conductive sensitivity, self-healing ability and good biocompatibility.
33965104	8	3	from	potential	1310:1318	arg1	monitor					1323:1329	monitor	1323:1329	monitor	1323:1329	Meanwhile, the current had a good linear relationship with the bending angles of hydrogels and pressure on hydrogels, which may provide a great potential in monitor both minor variations and large movements.
33965104	1	4	theme	conductive	281:290	arg1	sensitivity					292:302	high conductive sensitivity	276:302	high conductive sensitivity	276:302	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel was fabricated with high elongation, rapid recoverability, high conductive sensitivity, self-healing ability and good biocompatibility.
33965104	0	5	theme	conductive	86:95	arg1	hydrogel					110:117	conductive self-healing hydrogel	86:117	conductive self-healing hydrogel for sensor	86:128	Synergy coordination of cellulose-based dialdehyde and carboxyl with Fe3+ recoverable conductive self-healing hydrogel for sensor.
33965104	2	6	from	groups	515:520	arg1	complexations					401:413	the synergistic complexations	385:413	the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+	385:551	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	4	7	from	DACNFs	796:801	arg1	hydrogel					879:886	the dual ionic network cellulose-based composite conductive self-healing hydrogel	806:886	the dual ionic network cellulose-based composite conductive self-healing hydrogel	806:886	The self-healing efficiency increased with the addition of DACNFs in the dual ionic network cellulose-based composite conductive self-healing hydrogel.
33965104	1	8	theme	conductive	184:193	arg1	hydrogel					208:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel	131:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel	131:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel was fabricated with high elongation, rapid recoverability, high conductive sensitivity, self-healing ability and good biocompatibility.
33965104	4	9	theme	conductive	855:864	arg1	hydrogel					879:886	the dual ionic network cellulose-based composite conductive self-healing hydrogel	806:886	the dual ionic network cellulose-based composite conductive self-healing hydrogel	806:886	The self-healing efficiency increased with the addition of DACNFs in the dual ionic network cellulose-based composite conductive self-healing hydrogel.
33965104	5	10	theme	13.82	995:999	arg1	factor					985:990	a gauge factor	977:990	a gauge factor of 13.82 at strain within 1.6%	977:1021	The hydrogel configured for a wearable test and showed high stretching sensitivity with a gauge factor of 13.82 at strain within 1.6%.
33965104	8	11	with	relationship	1207:1218	arg1	angles					1237:1242	the bending angles	1225:1242	the bending angles of hydrogels	1225:1255	Meanwhile, the current had a good linear relationship with the bending angles of hydrogels and pressure on hydrogels, which may provide a great potential in monitor both minor variations and large movements.
33965104	2	12	with	nanofibers	482:491	arg1	Fe3+					548:551	Fe3+	548:551	Fe3+	548:551	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	4	13	from	addition	784:791	arg1	hydrogel					879:886	the dual ionic network cellulose-based composite conductive self-healing hydrogel	806:886	the dual ionic network cellulose-based composite conductive self-healing hydrogel	806:886	The self-healing efficiency increased with the addition of DACNFs in the dual ionic network cellulose-based composite conductive self-healing hydrogel.
33965104	1	14	theme	self-healing	305:316	arg1	ability					318:324	self-healing ability	305:324	self-healing ability	305:324	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel was fabricated with high elongation, rapid recoverability, high conductive sensitivity, self-healing ability and good biocompatibility.
33965104	5	15	theme	high	944:947	arg1	sensitivity					960:970	high stretching sensitivity	944:970	high stretching sensitivity	944:970	The hydrogel configured for a wearable test and showed high stretching sensitivity with a gauge factor of 13.82 at strain within 1.6%.
33965104	5	16	theme	gauge	979:983	arg1	factor					985:990	a gauge factor	977:990	a gauge factor of 13.82 at strain within 1.6%	977:1021	The hydrogel configured for a wearable test and showed high stretching sensitivity with a gauge factor of 13.82 at strain within 1.6%.
33965104	8	17	theme	bending	1229:1235	arg1	angles					1237:1242	the bending angles	1225:1242	the bending angles of hydrogels	1225:1255	Meanwhile, the current had a good linear relationship with the bending angles of hydrogels and pressure on hydrogels, which may provide a great potential in monitor both minor variations and large movements.
33965104	4	18	theme	DACNFs	796:801	arg1	addition					784:791	the addition	780:791	the addition of DACNFs in the dual ionic network cellulose-based composite conductive self-healing hydrogel	780:886	The self-healing efficiency increased with the addition of DACNFs in the dual ionic network cellulose-based composite conductive self-healing hydrogel.
33965104	8	19	theme	good	1195:1198	arg1	relationship					1207:1218	a good linear relationship	1193:1218	a good linear relationship with the bending angles of hydrogels	1193:1255	Meanwhile, the current had a good linear relationship with the bending angles of hydrogels and pressure on hydrogels, which may provide a great potential in monitor both minor variations and large movements.
33965104	1	20	theme	self-healing	195:206	arg1	hydrogel					208:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel	131:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel	131:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel was fabricated with high elongation, rapid recoverability, high conductive sensitivity, self-healing ability and good biocompatibility.
33965104	7	21	theme	300	1125:1127	arg1	%					1128:1128	%	1128:1128	%	1128:1128	GF were 0.696 between 20% and 300% strain, 0.837 within 300% and 500%.
33965104	3	22	theme	%	722:722	arg1	compression					724:734	80% compression	720:734	80% compression	720:734	The elongation (~1300%) of the hydrogel containing 1 wt% DACNFs was approximate 13-fold of the pure PAA hydrogel and can recover to original state within 2 min after 80% compression.
33965104	4	23	theme	self-healing	866:877	arg1	hydrogel					879:886	the dual ionic network cellulose-based composite conductive self-healing hydrogel	806:886	the dual ionic network cellulose-based composite conductive self-healing hydrogel	806:886	The self-healing efficiency increased with the addition of DACNFs in the dual ionic network cellulose-based composite conductive self-healing hydrogel.
33965104	2	24	theme	carboxyl	506:513	arg1	AA					539:540	AA	539:540	AA	539:540	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	2	24	theme	carboxyl	506:513	arg1	groups					515:520	carboxyl groups	506:520	carboxyl groups of acrylic acid (AA) with Fe3+	506:551	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	2	24	theme	carboxyl	506:513	arg1	acid					533:536	acrylic acid	525:536	acrylic acid (AA)	525:541	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	2	25	from	complexations	401:413	arg1	nanofibers					482:491	dialdehyde cellulose nanofibers	461:491	dialdehyde cellulose nanofibers (DACNFs)	461:500	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	2	25	from	complexations	401:413	arg1	AA					539:540	AA	539:540	AA	539:540	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	2	25	from	complexations	401:413	arg1	groups					515:520	carboxyl groups	506:520	carboxyl groups of acrylic acid (AA) with Fe3+	506:551	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	2	25	from	complexations	401:413	arg1	acid					533:536	acrylic acid	525:536	acrylic acid (AA)	525:541	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	2	25	from	complexations	401:413	arg1	DACNFs					494:499	DACNFs	494:499	DACNFs	494:499	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	8	26	theme	great	1304:1308	arg1	potential					1310:1318	a great potential	1302:1318	a great potential in monitor both minor variations and large movements	1302:1371	Meanwhile, the current had a good linear relationship with the bending angles of hydrogels and pressure on hydrogels, which may provide a great potential in monitor both minor variations and large movements.
33965104	5	27	theme	stretching	949:958	arg1	sensitivity					960:970	high stretching sensitivity	944:970	high stretching sensitivity	944:970	The hydrogel configured for a wearable test and showed high stretching sensitivity with a gauge factor of 13.82 at strain within 1.6%.
33965104	2	28	theme	groups	451:456	arg1	complexations					401:413	the synergistic complexations	385:413	the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+	385:551	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	0	29	theme	Synergy	0:6	arg1	coordination					8:19	Synergy coordination	0:19	Synergy coordination of cellulose-based dialdehyde and carboxyl with Fe3+	0:72	Synergy coordination of cellulose-based dialdehyde and carboxyl with Fe3+ recoverable conductive self-healing hydrogel for sensor.
33965104	8	30	contain	had	1189:1191	arg1	current					1181:1187	the current	1177:1187	the current	1177:1187	Meanwhile, the current had a good linear relationship with the bending angles of hydrogels and pressure on hydrogels, which may provide a great potential in monitor both minor variations and large movements.
33965104	8	30	contain	had	1189:1191	arg2	relationship					1207:1218	a good linear relationship	1193:1218	a good linear relationship with the bending angles of hydrogels	1193:1255	Meanwhile, the current had a good linear relationship with the bending angles of hydrogels and pressure on hydrogels, which may provide a great potential in monitor both minor variations and large movements.
33965104	8	30	contain	had	1189:1191	arg2	pressure					1261:1268	pressure	1261:1268	pressure	1261:1268	Meanwhile, the current had a good linear relationship with the bending angles of hydrogels and pressure on hydrogels, which may provide a great potential in monitor both minor variations and large movements.
33965104	3	31	theme	1 wt	605:608	arg1	DACNFs					611:616	1 wt% DACNFs	605:616	1 wt% DACNFs	605:616	The elongation (~1300%) of the hydrogel containing 1 wt% DACNFs was approximate 13-fold of the pure PAA hydrogel and can recover to original state within 2 min after 80% compression.
33965104	8	32	theme	linear	1200:1205	arg1	relationship					1207:1218	a good linear relationship	1193:1218	a good linear relationship with the bending angles of hydrogels	1193:1255	Meanwhile, the current had a good linear relationship with the bending angles of hydrogels and pressure on hydrogels, which may provide a great potential in monitor both minor variations and large movements.
33965104	2	33	theme	aldehyde	442:449	arg1	groups					451:456	new-fashioned bidentate aldehyde groups	418:456	new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+	418:551	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	0	34	theme	cellulose-based	24:38	arg1	dialdehyde					40:49	cellulose-based dialdehyde	24:49	cellulose-based dialdehyde	24:49	Synergy coordination of cellulose-based dialdehyde and carboxyl with Fe3+ recoverable conductive self-healing hydrogel for sensor.
33965104	2	35	theme	new-fashioned	418:430	arg1	groups					451:456	new-fashioned bidentate aldehyde groups	418:456	new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+	418:551	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	8	36	theme	minor	1336:1340	arg1	variations					1342:1351	minor variations	1336:1351	minor variations	1336:1351	Meanwhile, the current had a good linear relationship with the bending angles of hydrogels and pressure on hydrogels, which may provide a great potential in monitor both minor variations and large movements.
33965104	6	37	theme	gauge	1028:1032	arg1	factor					1034:1039	The gauge factor	1024:1039	The gauge factor (GF)	1024:1044	The gauge factor (GF) decrease with the incremental strain within 20%.
33965104	6	37	theme	gauge	1028:1032	arg1	GF					1042:1043	GF	1042:1043	GF	1042:1043	The gauge factor (GF) decrease with the incremental strain within 20%.
33965104	4	38	theme	self-healing	741:752	arg1	efficiency					754:763	The self-healing efficiency	737:763	The self-healing efficiency	737:763	The self-healing efficiency increased with the addition of DACNFs in the dual ionic network cellulose-based composite conductive self-healing hydrogel.
33965104	1	39	theme	good	330:333	arg1	biocompatibility					335:350	good biocompatibility	330:350	good biocompatibility	330:350	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel was fabricated with high elongation, rapid recoverability, high conductive sensitivity, self-healing ability and good biocompatibility.
33965104	2	40	with	groups	515:520	arg1	Fe3+					548:551	Fe3+	548:551	Fe3+	548:551	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	3	41	theme	hydrogel	585:592	arg1	approximate					622:632	approximate	622:632	approximate	622:632	The elongation (~1300%) of the hydrogel containing 1 wt% DACNFs was approximate 13-fold of the pure PAA hydrogel and can recover to original state within 2 min after 80% compression.
33965104	3	41	theme	hydrogel	585:592	arg1	elongation					558:567	The elongation	554:567	The elongation (~1300%) of the hydrogel containing 1 wt% DACNFs	554:616	The elongation (~1300%) of the hydrogel containing 1 wt% DACNFs was approximate 13-fold of the pure PAA hydrogel and can recover to original state within 2 min after 80% compression.
33965104	3	41	theme	hydrogel	585:592	arg1	%					575:575	~1300%	570:575	~1300%	570:575	The elongation (~1300%) of the hydrogel containing 1 wt% DACNFs was approximate 13-fold of the pure PAA hydrogel and can recover to original state within 2 min after 80% compression.
33965104	1	42	theme	high	237:240	arg1	elongation					242:251	high elongation	237:251	high elongation	237:251	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel was fabricated with high elongation, rapid recoverability, high conductive sensitivity, self-healing ability and good biocompatibility.
33965104	7	43	theme	%	1128:1128	arg1	strain					1130:1135	300% strain	1125:1135	300% strain	1125:1135	GF were 0.696 between 20% and 300% strain, 0.837 within 300% and 500%.
33965104	3	44	contain	containing	594:603	arg1	hydrogel					585:592	the hydrogel	581:592	the hydrogel containing 1 wt% DACNFs	581:616	The elongation (~1300%) of the hydrogel containing 1 wt% DACNFs was approximate 13-fold of the pure PAA hydrogel and can recover to original state within 2 min after 80% compression.
33965104	3	44	contain	containing	594:603	arg2	DACNFs					611:616	1 wt% DACNFs	605:616	1 wt% DACNFs	605:616	The elongation (~1300%) of the hydrogel containing 1 wt% DACNFs was approximate 13-fold of the pure PAA hydrogel and can recover to original state within 2 min after 80% compression.
33965104	4	45	theme	cellulose-based	829:843	arg1	hydrogel					879:886	the dual ionic network cellulose-based composite conductive self-healing hydrogel	806:886	the dual ionic network cellulose-based composite conductive self-healing hydrogel	806:886	The self-healing efficiency increased with the addition of DACNFs in the dual ionic network cellulose-based composite conductive self-healing hydrogel.
33965104	6	46	theme	incremental	1064:1074	arg1	strain					1076:1081	the incremental strain	1060:1081	the incremental strain	1060:1081	The gauge factor (GF) decrease with the incremental strain within 20%.
33965104	0	47	theme	dialdehyde	40:49	arg1	coordination					8:19	Synergy coordination	0:19	Synergy coordination of cellulose-based dialdehyde and carboxyl with Fe3+	0:72	Synergy coordination of cellulose-based dialdehyde and carboxyl with Fe3+ recoverable conductive self-healing hydrogel for sensor.
33965104	2	48	theme	acid	533:536	arg1	nanofibers					482:491	dialdehyde cellulose nanofibers	461:491	dialdehyde cellulose nanofibers (DACNFs)	461:500	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	2	48	theme	acid	533:536	arg1	AA					539:540	AA	539:540	AA	539:540	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	2	48	theme	acid	533:536	arg1	groups					515:520	carboxyl groups	506:520	carboxyl groups of acrylic acid (AA) with Fe3+	506:551	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	2	48	theme	acid	533:536	arg1	acid					533:536	acrylic acid	525:536	acrylic acid (AA)	525:541	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	2	48	theme	acid	533:536	arg1	DACNFs					494:499	DACNFs	494:499	DACNFs	494:499	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	3	49	theme	PAA	654:656	arg1	hydrogel					658:665	the pure PAA hydrogel	645:665	the pure PAA hydrogel	645:665	The elongation (~1300%) of the hydrogel containing 1 wt% DACNFs was approximate 13-fold of the pure PAA hydrogel and can recover to original state within 2 min after 80% compression.
33965104	4	50	theme	network	821:827	arg1	hydrogel					879:886	the dual ionic network cellulose-based composite conductive self-healing hydrogel	806:886	the dual ionic network cellulose-based composite conductive self-healing hydrogel	806:886	The self-healing efficiency increased with the addition of DACNFs in the dual ionic network cellulose-based composite conductive self-healing hydrogel.
33965104	2	51	from	groups	451:456	arg1	nanofibers					482:491	dialdehyde cellulose nanofibers	461:491	dialdehyde cellulose nanofibers (DACNFs)	461:500	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	2	51	from	groups	451:456	arg1	AA					539:540	AA	539:540	AA	539:540	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	2	51	from	groups	451:456	arg1	groups					515:520	carboxyl groups	506:520	carboxyl groups of acrylic acid (AA) with Fe3+	506:551	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	2	51	from	groups	451:456	arg1	acid					533:536	acrylic acid	525:536	acrylic acid (AA)	525:541	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	2	51	from	groups	451:456	arg1	DACNFs					494:499	DACNFs	494:499	DACNFs	494:499	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	2	52	theme	cellulose	472:480	arg1	nanofibers					482:491	dialdehyde cellulose nanofibers	461:491	dialdehyde cellulose nanofibers (DACNFs)	461:500	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	2	52	theme	cellulose	472:480	arg1	DACNFs					494:499	DACNFs	494:499	DACNFs	494:499	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	2	53	theme	acrylic	525:531	arg1	AA					539:540	AA	539:540	AA	539:540	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	2	53	theme	acrylic	525:531	arg1	acid					533:536	acrylic acid	525:536	acrylic acid (AA)	525:541	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	5	54	theme	wearable	919:926	arg1	test					928:931	a wearable test	917:931	a wearable test	917:931	The hydrogel configured for a wearable test and showed high stretching sensitivity with a gauge factor of 13.82 at strain within 1.6%.
33965104	3	55	theme	%	609:609	arg1	DACNFs					611:616	1 wt% DACNFs	605:616	1 wt% DACNFs	605:616	The elongation (~1300%) of the hydrogel containing 1 wt% DACNFs was approximate 13-fold of the pure PAA hydrogel and can recover to original state within 2 min after 80% compression.
33965104	4	56	from	hydrogel	879:886	arg1	addition					784:791	the addition	780:791	the addition of DACNFs in the dual ionic network cellulose-based composite conductive self-healing hydrogel	780:886	The self-healing efficiency increased with the addition of DACNFs in the dual ionic network cellulose-based composite conductive self-healing hydrogel.
33965104	2	57	theme	synergistic	389:399	arg1	complexations					401:413	the synergistic complexations	385:413	the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+	385:551	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	0	58	theme	carboxyl	55:62	arg1	coordination					8:19	Synergy coordination	0:19	Synergy coordination of cellulose-based dialdehyde and carboxyl with Fe3+	0:72	Synergy coordination of cellulose-based dialdehyde and carboxyl with Fe3+ recoverable conductive self-healing hydrogel for sensor.
33965104	2	59	theme	dialdehyde	461:470	arg1	nanofibers					482:491	dialdehyde cellulose nanofibers	461:491	dialdehyde cellulose nanofibers (DACNFs)	461:500	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	2	59	theme	dialdehyde	461:470	arg1	DACNFs					494:499	DACNFs	494:499	DACNFs	494:499	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	3	60	theme	original	686:693	arg1	state					695:699	original state	686:699	original state within 2 min	686:712	The elongation (~1300%) of the hydrogel containing 1 wt% DACNFs was approximate 13-fold of the pure PAA hydrogel and can recover to original state within 2 min after 80% compression.
33965104	4	61	theme	composite	845:853	arg1	hydrogel					879:886	the dual ionic network cellulose-based composite conductive self-healing hydrogel	806:886	the dual ionic network cellulose-based composite conductive self-healing hydrogel	806:886	The self-healing efficiency increased with the addition of DACNFs in the dual ionic network cellulose-based composite conductive self-healing hydrogel.
33965104	0	62	with	coordination	8:19	arg1	Fe3+					69:72	Fe3+	69:72	Fe3+	69:72	Synergy coordination of cellulose-based dialdehyde and carboxyl with Fe3+ recoverable conductive self-healing hydrogel for sensor.
33965104	1	63	theme	novel	133:137	arg1	hydrogel					208:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel	131:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel	131:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel was fabricated with high elongation, rapid recoverability, high conductive sensitivity, self-healing ability and good biocompatibility.
33965104	1	64	theme	network	150:156	arg1	hydrogel					208:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel	131:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel	131:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel was fabricated with high elongation, rapid recoverability, high conductive sensitivity, self-healing ability and good biocompatibility.
33965104	8	65	theme	large	1357:1361	arg1	movements					1363:1371	large movements	1357:1371	large movements	1357:1371	Meanwhile, the current had a good linear relationship with the bending angles of hydrogels and pressure on hydrogels, which may provide a great potential in monitor both minor variations and large movements.
33965104	1	66	theme	dual	139:142	arg1	hydrogel					208:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel	131:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel	131:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel was fabricated with high elongation, rapid recoverability, high conductive sensitivity, self-healing ability and good biocompatibility.
33965104	1	67	theme	rapid	254:258	arg1	recoverability					260:273	rapid recoverability	254:273	rapid recoverability	254:273	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel was fabricated with high elongation, rapid recoverability, high conductive sensitivity, self-healing ability and good biocompatibility.
33965104	8	68	with	pressure	1261:1268	arg1	angles					1237:1242	the bending angles	1225:1242	the bending angles of hydrogels	1225:1255	Meanwhile, the current had a good linear relationship with the bending angles of hydrogels and pressure on hydrogels, which may provide a great potential in monitor both minor variations and large movements.
33965104	3	69	theme	pure	649:652	arg1	hydrogel					658:665	the pure PAA hydrogel	645:665	the pure PAA hydrogel	645:665	The elongation (~1300%) of the hydrogel containing 1 wt% DACNFs was approximate 13-fold of the pure PAA hydrogel and can recover to original state within 2 min after 80% compression.
33965104	2	70	from	nanofibers	482:491	arg1	complexations					401:413	the synergistic complexations	385:413	the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+	385:551	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	1	71	theme	ionic	144:148	arg1	hydrogel					208:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel	131:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel	131:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel was fabricated with high elongation, rapid recoverability, high conductive sensitivity, self-healing ability and good biocompatibility.
33965104	8	72	theme	hydrogels	1247:1255	arg1	angles					1237:1242	the bending angles	1225:1242	the bending angles of hydrogels	1225:1255	Meanwhile, the current had a good linear relationship with the bending angles of hydrogels and pressure on hydrogels, which may provide a great potential in monitor both minor variations and large movements.
33965104	4	73	theme	ionic	815:819	arg1	hydrogel					879:886	the dual ionic network cellulose-based composite conductive self-healing hydrogel	806:886	the dual ionic network cellulose-based composite conductive self-healing hydrogel	806:886	The self-healing efficiency increased with the addition of DACNFs in the dual ionic network cellulose-based composite conductive self-healing hydrogel.
33965104	2	74	theme	bidentate	432:440	arg1	groups					451:456	new-fashioned bidentate aldehyde groups	418:456	new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+	418:551	The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe3+.
33965104	4	75	theme	dual	810:813	arg1	hydrogel					879:886	the dual ionic network cellulose-based composite conductive self-healing hydrogel	806:886	the dual ionic network cellulose-based composite conductive self-healing hydrogel	806:886	The self-healing efficiency increased with the addition of DACNFs in the dual ionic network cellulose-based composite conductive self-healing hydrogel.
33965104	1	76	theme	cellulose-based	158:172	arg1	hydrogel					208:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel	131:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel	131:215	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel was fabricated with high elongation, rapid recoverability, high conductive sensitivity, self-healing ability and good biocompatibility.
33965104	5	77	from	strain	1004:1009	arg1	factor					985:990	a gauge factor	977:990	a gauge factor of 13.82 at strain within 1.6%	977:1021	The hydrogel configured for a wearable test and showed high stretching sensitivity with a gauge factor of 13.82 at strain within 1.6%.
33965104	1	78	theme	high	276:279	arg1	sensitivity					292:302	high conductive sensitivity	276:302	high conductive sensitivity	276:302	A novel dual ionic network cellulose-based composite conductive self-healing hydrogel was fabricated with high elongation, rapid recoverability, high conductive sensitivity, self-healing ability and good biocompatibility.
32122515	8	0	theme	FTIR	927:930	arg1	techniques					932:941	FTIR techniques	927:941	FTIR techniques	927:941	%) were identified by employing zeta potential, XRD, conductometric test and FTIR techniques.
32122515	8	1	theme	zeta	882:885	arg1	potential					887:895	zeta potential	882:895	zeta potential	882:895	%) were identified by employing zeta potential, XRD, conductometric test and FTIR techniques.
32122515	0	2	theme	acid	98:101	arg1	hydrolysis					103:112	citric acid hydrolysis	91:112	citric acid hydrolysis	91:112	Preparation of bio-eco based cellulose nanomaterials from used disposal paper cups through citric acid hydrolysis.
32122515	7	3	theme	functional	791:800	arg1	groups					802:807	functional groups	791:807	functional groups	791:807	The colloidal stability, crystallinity index, presence of functional groups and elemental composition in CNCs (76 wt.
32122515	0	4	theme	citric	91:96	arg1	hydrolysis					103:112	citric acid hydrolysis	91:112	citric acid hydrolysis	91:112	Preparation of bio-eco based cellulose nanomaterials from used disposal paper cups through citric acid hydrolysis.
32122515	0	5	used	used	58:61	arg2	Preparation					0:10	Preparation	0:10	Preparation of bio-eco based cellulose nanomaterials from	0:56	Preparation of bio-eco based cellulose nanomaterials from used disposal paper cups through citric acid hydrolysis.
32122515	6	6	theme	acid	711:714	arg1	%					706:706	%	706:706	% of acid	706:714	% of acid hydrolyzed CNCs.
32122515	6	6	theme	acid	711:714	arg1	acid					711:714	acid	711:714	acid	711:714	% of acid hydrolyzed CNCs.
32122515	5	7	theme	13.7 ± 0.6 nm	665:677	arg1	width					656:660	a width	654:660	a width of 13.7 ± 0.6 nm	654:677	%) has an appearance of rod-like structure with a width of 13.7 ± 0.6 nm which results due to 76 wt.
32122515	0	8	theme	nanomaterials	39:51	arg1	Preparation					0:10	Preparation	0:10	Preparation of bio-eco based cellulose nanomaterials from	0:56	Preparation of bio-eco based cellulose nanomaterials from used disposal paper cups through citric acid hydrolysis.
32122515	7	9	theme	crystallinity	758:770	arg1	stability					747:755	The colloidal stability	733:755	The colloidal stability	733:755	The colloidal stability, crystallinity index, presence of functional groups and elemental composition in CNCs (76 wt.
32122515	7	9	theme	crystallinity	758:770	arg1	index					772:776	crystallinity index	758:776	crystallinity index	758:776	The colloidal stability, crystallinity index, presence of functional groups and elemental composition in CNCs (76 wt.
32122515	10	10	theme	thermo-gravimetric	1013:1030	arg1	analysis					1032:1039	thermo-gravimetric analysis	1013:1039	thermo-gravimetric analysis	1013:1039	%) was investigated by thermo-gravimetric analysis.
32122515	3	11	theme	acid	507:510	arg1	concentration					512:524	acid concentration	507:524	acid concentration	507:524	The effect of acid concentration on microstructure and yield of CNCs are highlighted.
32122515	1	12	theme	recent	251:256	arg1	years					258:262	recent years	251:262	recent years	251:262	The Used Disposal Paper Cups (UDPCs) have become a concern to the solid waste management sector as scientists triggered the problems in recent years, to proceed forward in developing the process for this issue.
32122515	3	13	theme	concentration	512:524	arg1	effect					497:502	The effect	493:502	The effect of acid concentration on microstructure and yield of CNCs	493:560	The effect of acid concentration on microstructure and yield of CNCs are highlighted.
32122515	0	14	theme	based	23:27	arg1	nanomaterials					39:51	bio-eco based cellulose nanomaterials	15:51	bio-eco based cellulose nanomaterials	15:51	Preparation of bio-eco based cellulose nanomaterials from used disposal paper cups through citric acid hydrolysis.
32122515	1	15	from	problems	239:246	arg1	years					258:262	recent years	251:262	recent years	251:262	The Used Disposal Paper Cups (UDPCs) have become a concern to the solid waste management sector as scientists triggered the problems in recent years, to proceed forward in developing the process for this issue.
32122515	7	16	dep	CNCs	838:841	arg1	76 wt					844:848	76 wt	844:848	76 wt	844:848	The colloidal stability, crystallinity index, presence of functional groups and elemental composition in CNCs (76 wt.
32122515	4	17	dep	yield	593:597	arg1	55 wt					600:604	55 wt	600:604	55 wt	600:604	The optimized yield (55 wt.
32122515	5	18	with	structure	639:647	arg1	width					656:660	a width	654:660	a width of 13.7 ± 0.6 nm	654:677	%) has an appearance of rod-like structure with a width of 13.7 ± 0.6 nm which results due to 76 wt.
32122515	0	19	theme	bio-eco	15:21	arg1	nanomaterials					39:51	bio-eco based cellulose nanomaterials	15:51	bio-eco based cellulose nanomaterials	15:51	Preparation of bio-eco based cellulose nanomaterials from used disposal paper cups through citric acid hydrolysis.
32122515	5	20	theme	rod-like	630:637	arg1	structure					639:647	rod-like structure	630:647	rod-like structure with a width of 13.7 ± 0.6 nm	630:677	%) has an appearance of rod-like structure with a width of 13.7 ± 0.6 nm which results due to 76 wt.
32122515	2	21	theme	Cellulose	420:428	arg1	CNCs					444:447	CNCs	444:447	CNCs	444:447	Based on this concern, the present study emphasizes on the isolation of a novel bio-eco based Cellulose NanoCrystals (CNCs) from UDPCs through citric acid hydrolysis.
32122515	2	21	theme	Cellulose	420:428	arg1	NanoCrystals					430:441	Cellulose NanoCrystals	420:441	Cellulose NanoCrystals (CNCs) from UDPCs	420:459	Based on this concern, the present study emphasizes on the isolation of a novel bio-eco based Cellulose NanoCrystals (CNCs) from UDPCs through citric acid hydrolysis.
32122515	5	22	theme	structure	639:647	arg1	appearance					616:625	an appearance	613:625	an appearance of rod-like structure with a width of 13.7 ± 0.6 nm which results due to 76 wt	613:704	%) has an appearance of rod-like structure with a width of 13.7 ± 0.6 nm which results due to 76 wt.
32122515	4	23	theme	optimized	583:591	arg1	yield					593:597	The optimized yield	579:597	The optimized yield (55 wt	579:604	The optimized yield (55 wt.
32122515	7	24	theme	colloidal	737:745	arg1	stability					747:755	The colloidal stability	733:755	The colloidal stability	733:755	The colloidal stability, crystallinity index, presence of functional groups and elemental composition in CNCs (76 wt.
32122515	7	24	theme	colloidal	737:745	arg1	index					772:776	crystallinity index	758:776	crystallinity index	758:776	The colloidal stability, crystallinity index, presence of functional groups and elemental composition in CNCs (76 wt.
32122515	7	24	theme	colloidal	737:745	arg1	presence					779:786	presence	779:786	presence of functional groups and elemental composition in CNCs (76 wt	779:848	The colloidal stability, crystallinity index, presence of functional groups and elemental composition in CNCs (76 wt.
32122515	1	25	theme	solid	181:185	arg1	sector					204:209	the solid waste management sector	177:209	the solid waste management sector	177:209	The Used Disposal Paper Cups (UDPCs) have become a concern to the solid waste management sector as scientists triggered the problems in recent years, to proceed forward in developing the process for this issue.
32122515	0	26	theme	cellulose	29:37	arg1	nanomaterials					39:51	bio-eco based cellulose nanomaterials	15:51	bio-eco based cellulose nanomaterials	15:51	Preparation of bio-eco based cellulose nanomaterials from used disposal paper cups through citric acid hydrolysis.
32122515	2	27	theme	acid	476:479	arg1	hydrolysis					481:490	citric acid hydrolysis	469:490	citric acid hydrolysis	469:490	Based on this concern, the present study emphasizes on the isolation of a novel bio-eco based Cellulose NanoCrystals (CNCs) from UDPCs through citric acid hydrolysis.
32122515	8	28	theme	conductometric	903:916	arg1	test					918:921	conductometric test	903:921	conductometric test	903:921	%) were identified by employing zeta potential, XRD, conductometric test and FTIR techniques.
32122515	9	29	dep	CNCs	978:981	arg1	76 wt					984:988	76 wt	984:988	76 wt	984:988	Finally, the thermal stability of CNCs (76 wt.
32122515	1	30	theme	waste	187:191	arg1	sector					204:209	the solid waste management sector	177:209	the solid waste management sector	177:209	The Used Disposal Paper Cups (UDPCs) have become a concern to the solid waste management sector as scientists triggered the problems in recent years, to proceed forward in developing the process for this issue.
32122515	2	31	theme	bio-eco	406:412	arg1	isolation					385:393	the isolation	381:393	the isolation of a novel bio-eco based Cellulose NanoCrystals (CNCs) from UDPCs through citric acid hydrolysis	381:490	Based on this concern, the present study emphasizes on the isolation of a novel bio-eco based Cellulose NanoCrystals (CNCs) from UDPCs through citric acid hydrolysis.
32122515	2	32	theme	citric	469:474	arg1	hydrolysis					481:490	citric acid hydrolysis	469:490	citric acid hydrolysis	469:490	Based on this concern, the present study emphasizes on the isolation of a novel bio-eco based Cellulose NanoCrystals (CNCs) from UDPCs through citric acid hydrolysis.
32122515	7	33	attach	presence	779:786	arg2	composition					823:833	elemental composition	813:833	elemental composition	813:833	The colloidal stability, crystallinity index, presence of functional groups and elemental composition in CNCs (76 wt.
32122515	7	33	attach	presence	779:786	arg1	CNCs					838:841	CNCs	838:841	CNCs (76 wt	838:848	The colloidal stability, crystallinity index, presence of functional groups and elemental composition in CNCs (76 wt.
32122515	7	33	attach	presence	779:786	arg2	groups					802:807	functional groups	791:807	functional groups	791:807	The colloidal stability, crystallinity index, presence of functional groups and elemental composition in CNCs (76 wt.
32122515	2	34	theme	novel	400:404	arg1	bio-eco					406:412	a novel bio-eco	398:412	a novel bio-eco based Cellulose NanoCrystals (CNCs) from UDPCs through citric acid hydrolysis	398:490	Based on this concern, the present study emphasizes on the isolation of a novel bio-eco based Cellulose NanoCrystals (CNCs) from UDPCs through citric acid hydrolysis.
32122515	5	35	contain	has	609:611	arg2	appearance					616:625	an appearance	613:625	an appearance of rod-like structure with a width of 13.7 ± 0.6 nm which results due to 76 wt	613:704	%) has an appearance of rod-like structure with a width of 13.7 ± 0.6 nm which results due to 76 wt.
32122515	5	35	contain	has	609:611	arg1	%					606:606	%)	606:607	%)	606:607	%) has an appearance of rod-like structure with a width of 13.7 ± 0.6 nm which results due to 76 wt.
32122515	2	36	from	UDPCs	455:459	arg1	CNCs					444:447	CNCs	444:447	CNCs	444:447	Based on this concern, the present study emphasizes on the isolation of a novel bio-eco based Cellulose NanoCrystals (CNCs) from UDPCs through citric acid hydrolysis.
32122515	2	36	from	UDPCs	455:459	arg1	NanoCrystals					430:441	Cellulose NanoCrystals	420:441	Cellulose NanoCrystals (CNCs) from UDPCs	420:459	Based on this concern, the present study emphasizes on the isolation of a novel bio-eco based Cellulose NanoCrystals (CNCs) from UDPCs through citric acid hydrolysis.
32122515	1	37	theme	Used	119:122	arg1	UDPCs					145:149	The Used Disposal Paper Cups (UDPCs)	115:150	The Used Disposal Paper Cups (UDPCs)	115:150	The Used Disposal Paper Cups (UDPCs) have become a concern to the solid waste management sector as scientists triggered the problems in recent years, to proceed forward in developing the process for this issue.
32122515	3	38	from	effect	497:502	arg1	yield					548:552	yield	548:552	yield	548:552	The effect of acid concentration on microstructure and yield of CNCs are highlighted.
32122515	3	38	from	effect	497:502	arg1	microstructure					529:542	microstructure	529:542	microstructure	529:542	The effect of acid concentration on microstructure and yield of CNCs are highlighted.
32122515	7	39	from	presence	779:786	arg1	CNCs					838:841	CNCs	838:841	CNCs (76 wt	838:848	The colloidal stability, crystallinity index, presence of functional groups and elemental composition in CNCs (76 wt.
32122515	9	40	theme	thermal	957:963	arg1	stability					965:973	the thermal stability	953:973	the thermal stability of CNCs (76 wt	953:988	Finally, the thermal stability of CNCs (76 wt.
32122515	1	41	theme	Disposal	124:131	arg1	UDPCs					145:149	The Used Disposal Paper Cups (UDPCs)	115:150	The Used Disposal Paper Cups (UDPCs)	115:150	The Used Disposal Paper Cups (UDPCs) have become a concern to the solid waste management sector as scientists triggered the problems in recent years, to proceed forward in developing the process for this issue.
32122515	1	42	theme	management	193:202	arg1	sector					204:209	the solid waste management sector	177:209	the solid waste management sector	177:209	The Used Disposal Paper Cups (UDPCs) have become a concern to the solid waste management sector as scientists triggered the problems in recent years, to proceed forward in developing the process for this issue.
32122515	1	43	theme	Paper	133:137	arg1	UDPCs					145:149	The Used Disposal Paper Cups (UDPCs)	115:150	The Used Disposal Paper Cups (UDPCs)	115:150	The Used Disposal Paper Cups (UDPCs) have become a concern to the solid waste management sector as scientists triggered the problems in recent years, to proceed forward in developing the process for this issue.
32122515	7	44	theme	elemental	813:821	arg1	composition					823:833	elemental composition	813:833	elemental composition	813:833	The colloidal stability, crystallinity index, presence of functional groups and elemental composition in CNCs (76 wt.
32122515	9	45	theme	CNCs	978:981	arg1	stability					965:973	the thermal stability	953:973	the thermal stability of CNCs (76 wt	953:988	Finally, the thermal stability of CNCs (76 wt.
32122515	0	46	theme	paper	72:76	arg1	cups					78:81	disposal paper cups	63:81	disposal paper cups	63:81	Preparation of bio-eco based cellulose nanomaterials from used disposal paper cups through citric acid hydrolysis.
32122515	2	47	theme	present	353:359	arg1	study					361:365	the present study	349:365	the present study	349:365	Based on this concern, the present study emphasizes on the isolation of a novel bio-eco based Cellulose NanoCrystals (CNCs) from UDPCs through citric acid hydrolysis.
32122515	1	48	theme	Cups	139:142	arg1	UDPCs					145:149	The Used Disposal Paper Cups (UDPCs)	115:150	The Used Disposal Paper Cups (UDPCs)	115:150	The Used Disposal Paper Cups (UDPCs) have become a concern to the solid waste management sector as scientists triggered the problems in recent years, to proceed forward in developing the process for this issue.
32122515	7	49	theme	composition	823:833	arg1	stability					747:755	The colloidal stability	733:755	The colloidal stability	733:755	The colloidal stability, crystallinity index, presence of functional groups and elemental composition in CNCs (76 wt.
32122515	7	49	theme	composition	823:833	arg1	presence					779:786	presence	779:786	presence of functional groups and elemental composition in CNCs (76 wt	779:848	The colloidal stability, crystallinity index, presence of functional groups and elemental composition in CNCs (76 wt.
32122515	0	50	theme	disposal	63:70	arg1	cups					78:81	disposal paper cups	63:81	disposal paper cups	63:81	Preparation of bio-eco based cellulose nanomaterials from used disposal paper cups through citric acid hydrolysis.
32122515	3	51	theme	CNCs	557:560	arg1	yield					548:552	yield	548:552	yield	548:552	The effect of acid concentration on microstructure and yield of CNCs are highlighted.
32122515	3	51	theme	CNCs	557:560	arg1	microstructure					529:542	microstructure	529:542	microstructure	529:542	The effect of acid concentration on microstructure and yield of CNCs are highlighted.
32122515	7	52	theme	groups	802:807	arg1	stability					747:755	The colloidal stability	733:755	The colloidal stability	733:755	The colloidal stability, crystallinity index, presence of functional groups and elemental composition in CNCs (76 wt.
32122515	7	52	theme	groups	802:807	arg1	presence					779:786	presence	779:786	presence of functional groups and elemental composition in CNCs (76 wt	779:848	The colloidal stability, crystallinity index, presence of functional groups and elemental composition in CNCs (76 wt.
32105688	0	0	theme	alginate	85:92	arg1	nanoparticles					111:123	cellulose, sodium alginate and copper oxide nanoparticles	67:123	cellulose, sodium alginate and copper oxide nanoparticles for food packaging application	67:154	Physical and bioactivities of biopolymeric films incorporated with cellulose, sodium alginate and copper oxide nanoparticles for food packaging application.
32105688	1	1	theme	embedded	311:318	arg1	combination					242:252	the different combination	228:252	the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs)	228:360	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	5	2	theme	SA-CNW-CuNPs	611:622	arg1	film					624:627	SA-CNW-CuNPs film	611:627	SA-CNW-CuNPs film	611:627	Cross-linking and functional relationship of SA-CNW-CuNPs film were confirmed by adopting characterization including SEM, FTIR, EDS and XRD.
32105688	6	3	theme	antibacterial	782:794	arg1	activity					796:803	promising antibacterial activity	772:803	promising antibacterial activity against several pathogens	772:829	The film composed with CNW (0.5%)-SA (3%)-CuNPs (5 mM) exhibited promising antibacterial activity against several pathogens in terms of higher zone of inhibition against S. aureus (27.49 ± 0.91 mm), E. coli (12.12 ± 0.58 mm), Salmonella sp.
32105688	1	4	theme	present	161:167	arg1	work					169:172	The present work	157:172	The present work	157:172	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	6	5	theme	several	813:819	arg1	pathogens					821:829	several pathogens	813:829	several pathogens	813:829	The film composed with CNW (0.5%)-SA (3%)-CuNPs (5 mM) exhibited promising antibacterial activity against several pathogens in terms of higher zone of inhibition against S. aureus (27.49 ± 0.91 mm), E. coli (12.12 ± 0.58 mm), Salmonella sp.
32105688	10	6	theme	-SA	1233:1235	arg1	5 mM					1251:1254	5 mM	1251:1254	5 mM	1251:1254	Also, the optimized composition of film composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) was preventing the microbial contamination in fresh cut pepper (FCP).
32105688	10	6	theme	-SA	1233:1235	arg1	NPs					1246:1248	CNW (0.5%)-SA (3%)-CuO NPs	1223:1248	CNW (0.5%)-SA (3%)-CuO NPs (5 mM)	1223:1255	Also, the optimized composition of film composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) was preventing the microbial contamination in fresh cut pepper (FCP).
32105688	0	7	theme	sodium	78:83	arg1	alginate					85:92	sodium alginate	78:92	sodium alginate	78:92	Physical and bioactivities of biopolymeric films incorporated with cellulose, sodium alginate and copper oxide nanoparticles for food packaging application.
32105688	9	8	theme	challenging	1098:1108	arg1	activity					1122:1129	the challenging antioxidant activity	1094:1129	the challenging antioxidant activity in terms of DPPH and ABTS scavenging	1094:1166	In addition, CNW (0.5%)-SA(3%)-CuO NPs (5 mM) film showed the challenging antioxidant activity in terms of DPPH and ABTS scavenging.
32105688	5	9	theme	film	624:627	arg1	relationship					595:606	functional relationship	584:606	functional relationship	584:606	Cross-linking and functional relationship of SA-CNW-CuNPs film were confirmed by adopting characterization including SEM, FTIR, EDS and XRD.
32105688	5	9	theme	film	624:627	arg1	Cross-linking					566:578	Cross-linking	566:578	Cross-linking	566:578	Cross-linking and functional relationship of SA-CNW-CuNPs film were confirmed by adopting characterization including SEM, FTIR, EDS and XRD.
32105688	9	10	theme	CNW	1049:1051	arg1	-SA					1059:1061	CNW (0.5%)-SA	1049:1061	CNW (0.5%)-SA(3%)-CuO NPs (5 mM) film	1049:1085	In addition, CNW (0.5%)-SA(3%)-CuO NPs (5 mM) film showed the challenging antioxidant activity in terms of DPPH and ABTS scavenging.
32105688	9	10	theme	CNW	1049:1051	arg1	%					1064:1064	3%	1063:1064	3%	1063:1064	In addition, CNW (0.5%)-SA(3%)-CuO NPs (5 mM) film showed the challenging antioxidant activity in terms of DPPH and ABTS scavenging.
32105688	11	11	theme	conventional	1508:1519	arg1	packaging					1526:1534	conventional food packaging	1508:1534	conventional food packaging	1508:1534	Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
32105688	1	12	theme	sodium	257:262	arg1	SA					274:275	SA	274:275	SA	274:275	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	1	12	theme	sodium	257:262	arg1	alginate					264:271	sodium alginate	257:271	sodium alginate (SA)	257:276	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	0	13	theme	copper	98:103	arg1	oxide					105:109	copper oxide	98:109	copper oxide	98:109	Physical and bioactivities of biopolymeric films incorporated with cellulose, sodium alginate and copper oxide nanoparticles for food packaging application.
32105688	1	14	theme	copper	325:330	arg1	oxide					332:336	copper oxide	325:336	copper oxide nanoparticles (CuO NPs)	325:360	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	1	15	with	embedded	311:318	arg1	nanoparticles					338:350	copper oxide nanoparticles	325:350	copper oxide nanoparticles (CuO NPs)	325:360	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	1	15	with	embedded	311:318	arg1	NPs					357:359	CuO NPs	353:359	CuO NPs	353:359	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	1	16	theme	alginate	264:271	arg1	combination					242:252	the different combination	228:252	the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs)	228:360	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	1	17	theme	oxide	332:336	arg1	nanoparticles					338:350	copper oxide nanoparticles	325:350	copper oxide nanoparticles (CuO NPs)	325:360	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	1	17	theme	oxide	332:336	arg1	NPs					357:359	CuO NPs	353:359	CuO NPs	353:359	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	4	18	theme	microbial	533:541	arg1	contamination					543:555	the microbial contamination	529:555	the microbial contamination to food	529:563	CuO NPs prevent the microbial contamination to food.
32105688	10	19	theme	optimized	1179:1187	arg1	composition					1189:1199	the optimized composition	1175:1199	the optimized composition of film composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM)	1175:1255	Also, the optimized composition of film composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) was preventing the microbial contamination in fresh cut pepper (FCP).
32105688	6	20	dep	CNW	730:732	arg1	%					738:738	0.5%	735:738	0.5%	735:738	The film composed with CNW (0.5%)-SA (3%)-CuNPs (5 mM) exhibited promising antibacterial activity against several pathogens in terms of higher zone of inhibition against S. aureus (27.49 ± 0.91 mm), E. coli (12.12 ± 0.58 mm), Salmonella sp.
32105688	0	21	theme	oxide	105:109	arg1	nanoparticles					111:123	cellulose, sodium alginate and copper oxide nanoparticles	67:123	cellulose, sodium alginate and copper oxide nanoparticles for food packaging application	67:154	Physical and bioactivities of biopolymeric films incorporated with cellulose, sodium alginate and copper oxide nanoparticles for food packaging application.
32105688	11	22	theme	-SA	1366:1368	arg1	NPs					1379:1381	CNW (0.5%)-SA (3%)-CuO NPs	1356:1381	CNW (0.5%)-SA (3%)-CuO NPs (5 mM)	1356:1388	Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
32105688	11	22	theme	-SA	1366:1368	arg1	5 mM					1384:1387	5 mM	1384:1387	5 mM	1384:1387	Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
32105688	5	23	theme	functional	584:593	arg1	relationship					595:606	functional relationship	584:606	functional relationship	584:606	Cross-linking and functional relationship of SA-CNW-CuNPs film were confirmed by adopting characterization including SEM, FTIR, EDS and XRD.
32105688	10	24	theme	-CuO	1241:1244	arg1	5 mM					1251:1254	5 mM	1251:1254	5 mM	1251:1254	Also, the optimized composition of film composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) was preventing the microbial contamination in fresh cut pepper (FCP).
32105688	10	24	theme	-CuO	1241:1244	arg1	NPs					1246:1248	CNW (0.5%)-SA (3%)-CuO NPs	1223:1248	CNW (0.5%)-SA (3%)-CuO NPs (5 mM)	1223:1255	Also, the optimized composition of film composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) was preventing the microbial contamination in fresh cut pepper (FCP).
32105688	3	25	theme	moisture	481:488	arg1	penetration					490:500	moisture penetration	481:500	moisture penetration into food	481:510	The CNW improves barrier properties of the film to limit moisture penetration into food.
32105688	1	26	theme	CuO	353:355	arg1	nanoparticles					338:350	copper oxide nanoparticles	325:350	copper oxide nanoparticles (CuO NPs)	325:360	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	1	26	theme	CuO	353:355	arg1	NPs					357:359	CuO NPs	353:359	CuO NPs	353:359	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	10	27	theme	CNW	1223:1225	arg1	-SA					1233:1235	CNW (0.5%)-SA	1223:1235	CNW (0.5%)-SA (3%)-CuO NPs (5 mM)	1223:1255	Also, the optimized composition of film composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) was preventing the microbial contamination in fresh cut pepper (FCP).
32105688	10	27	theme	CNW	1223:1225	arg1	%					1239:1239	3%	1238:1239	3%	1238:1239	Also, the optimized composition of film composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) was preventing the microbial contamination in fresh cut pepper (FCP).
32105688	6	28	dep	-CuNPs	748:753	arg1	-SA					740:742	-SA	740:742	-SA	740:742	The film composed with CNW (0.5%)-SA (3%)-CuNPs (5 mM) exhibited promising antibacterial activity against several pathogens in terms of higher zone of inhibition against S. aureus (27.49 ± 0.91 mm), E. coli (12.12 ± 0.58 mm), Salmonella sp.
32105688	1	29	theme	antibacterial	187:199	arg1	APF					217:219	APF	217:219	APF	217:219	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	1	29	theme	antibacterial	187:199	arg1	film					211:214	the antibacterial polymeric film	183:214	the antibacterial polymeric film (APF)	183:220	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	9	30	theme	DPPH	1143:1146	arg1	terms					1134:1138	terms	1134:1138	terms of DPPH and ABTS scavenging	1134:1166	In addition, CNW (0.5%)-SA(3%)-CuO NPs (5 mM) film showed the challenging antioxidant activity in terms of DPPH and ABTS scavenging.
32105688	6	31	dep	-SA	740:742	arg1	CNW					730:732	CNW	730:732	CNW	730:732	The film composed with CNW (0.5%)-SA (3%)-CuNPs (5 mM) exhibited promising antibacterial activity against several pathogens in terms of higher zone of inhibition against S. aureus (27.49 ± 0.91 mm), E. coli (12.12 ± 0.58 mm), Salmonella sp.
32105688	6	31	dep	-SA	740:742	arg1	%					746:746	3%	745:746	3%	745:746	The film composed with CNW (0.5%)-SA (3%)-CuNPs (5 mM) exhibited promising antibacterial activity against several pathogens in terms of higher zone of inhibition against S. aureus (27.49 ± 0.91 mm), E. coli (12.12 ± 0.58 mm), Salmonella sp.
32105688	1	32	theme	polymeric	201:209	arg1	APF					217:219	APF	217:219	APF	217:219	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	1	32	theme	polymeric	201:209	arg1	film					211:214	the antibacterial polymeric film	183:214	the antibacterial polymeric film (APF)	183:220	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	9	33	from	activity	1122:1129	arg1	terms					1134:1138	terms	1134:1138	terms of DPPH and ABTS scavenging	1134:1166	In addition, CNW (0.5%)-SA(3%)-CuO NPs (5 mM) film showed the challenging antioxidant activity in terms of DPPH and ABTS scavenging.
32105688	0	34	theme	food	129:132	arg1	application					144:154	food packaging application	129:154	food packaging application	129:154	Physical and bioactivities of biopolymeric films incorporated with cellulose, sodium alginate and copper oxide nanoparticles for food packaging application.
32105688	11	35	from	limitations	1493:1503	arg1	packaging					1526:1534	conventional food packaging	1508:1534	conventional food packaging	1508:1534	Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
32105688	11	36	theme	food	1463:1466	arg1	industry					1468:1475	food industry	1463:1475	food industry	1463:1475	Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
32105688	10	37	theme	film	1204:1207	arg1	composition					1189:1199	the optimized composition	1175:1199	the optimized composition of film composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM)	1175:1255	Also, the optimized composition of film composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) was preventing the microbial contamination in fresh cut pepper (FCP).
32105688	4	38	theme	CuO	513:515	arg1	NPs					517:519	CuO NPs	513:519	CuO NPs	513:519	CuO NPs prevent the microbial contamination to food.
32105688	9	39	theme	ABTS	1152:1155	arg1	scavenging					1157:1166	ABTS scavenging	1152:1166	ABTS scavenging	1152:1166	In addition, CNW (0.5%)-SA(3%)-CuO NPs (5 mM) film showed the challenging antioxidant activity in terms of DPPH and ABTS scavenging.
32105688	6	40	theme	promising	772:780	arg1	activity					796:803	promising antibacterial activity	772:803	promising antibacterial activity against several pathogens	772:829	The film composed with CNW (0.5%)-SA (3%)-CuNPs (5 mM) exhibited promising antibacterial activity against several pathogens in terms of higher zone of inhibition against S. aureus (27.49 ± 0.91 mm), E. coli (12.12 ± 0.58 mm), Salmonella sp.
32105688	11	41	theme	food	1521:1524	arg1	packaging					1526:1534	conventional food packaging	1508:1534	conventional food packaging	1508:1534	Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
32105688	0	42	theme	films	43:47	arg1	Physical					0:7	Physical	0:7	Physical	0:7	Physical and bioactivities of biopolymeric films incorporated with cellulose, sodium alginate and copper oxide nanoparticles for food packaging application.
32105688	1	43	theme	cellulose	282:290	arg1	whisker					297:303	cellulose nano whisker	282:303	cellulose nano whisker (CNW)	282:309	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	1	43	theme	cellulose	282:290	arg1	CNW					306:308	CNW	306:308	CNW	306:308	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	6	44	theme	Salmonella	933:942	arg1	sp					944:945	Salmonella sp	933:945	Salmonella sp	933:945	The film composed with CNW (0.5%)-SA (3%)-CuNPs (5 mM) exhibited promising antibacterial activity against several pathogens in terms of higher zone of inhibition against S. aureus (27.49 ± 0.91 mm), E. coli (12.12 ± 0.58 mm), Salmonella sp.
32105688	6	44	theme	Salmonella	933:942	arg1	aureus					880:885	S. aureus	877:885	S. aureus (27.49 ± 0.91 mm)	877:903	The film composed with CNW (0.5%)-SA (3%)-CuNPs (5 mM) exhibited promising antibacterial activity against several pathogens in terms of higher zone of inhibition against S. aureus (27.49 ± 0.91 mm), E. coli (12.12 ± 0.58 mm), Salmonella sp.
32105688	0	45	theme	biopolymeric	30:41	arg1	films					43:47	biopolymeric films	30:47	biopolymeric films	30:47	Physical and bioactivities of biopolymeric films incorporated with cellulose, sodium alginate and copper oxide nanoparticles for food packaging application.
32105688	1	46	theme	nano	292:295	arg1	whisker					297:303	cellulose nano whisker	282:303	cellulose nano whisker (CNW)	282:309	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	1	46	theme	nano	292:295	arg1	CNW					306:308	CNW	306:308	CNW	306:308	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	0	47	theme	packaging	134:142	arg1	application					144:154	food packaging application	129:154	food packaging application	129:154	Physical and bioactivities of biopolymeric films incorporated with cellulose, sodium alginate and copper oxide nanoparticles for food packaging application.
32105688	10	48	theme	microbial	1276:1284	arg1	contamination					1286:1298	the microbial contamination	1272:1298	the microbial contamination	1272:1298	Also, the optimized composition of film composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) was preventing the microbial contamination in fresh cut pepper (FCP).
32105688	11	49	theme	food	1416:1419	arg1	FPS					1439:1441	FPS	1439:1441	FPS	1439:1441	Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
32105688	11	49	theme	food	1416:1419	arg1	system					1431:1436	an active food packaging system	1406:1436	an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging	1406:1534	Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
32105688	11	49	theme	food	1416:1419	arg1	APF					1338:1340	APF	1338:1340	APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM)	1338:1388	Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
32105688	10	50	theme	cut	1309:1311	arg1	pepper					1313:1318	fresh cut pepper	1303:1318	fresh cut pepper (FCP)	1303:1324	Also, the optimized composition of film composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) was preventing the microbial contamination in fresh cut pepper (FCP).
32105688	10	50	theme	cut	1309:1311	arg1	FCP					1321:1323	FCP	1321:1323	FCP	1321:1323	Also, the optimized composition of film composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) was preventing the microbial contamination in fresh cut pepper (FCP).
32105688	1	51	theme	whisker	297:303	arg1	combination					242:252	the different combination	228:252	the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs)	228:360	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	7	52	dep	25.21 ± 1.05 mm	949:963	arg1	23.35 ± 0.45 mm					980:994	23.35 ± 0.45 mm	980:994	23.35 ± 0.45 mm	980:994	(25.21 ± 1.05 mm), C. albicans (23.35 ± 0.45 mm) and Trichoderma spp.
32105688	7	52	dep	25.21 ± 1.05 mm	949:963	arg1	albicans					970:977	C. albicans	967:977	C. albicans (23.35 ± 0.45 mm)	967:995	(25.21 ± 1.05 mm), C. albicans (23.35 ± 0.45 mm) and Trichoderma spp.
32105688	7	52	dep	25.21 ± 1.05 mm	949:963	arg1	spp					1013:1015	Trichoderma spp	1001:1015	Trichoderma spp	1001:1015	(25.21 ± 1.05 mm), C. albicans (23.35 ± 0.45 mm) and Trichoderma spp.
32105688	3	53	theme	barrier	441:447	arg1	properties					449:458	barrier properties	441:458	barrier properties of the film	441:470	The CNW improves barrier properties of the film to limit moisture penetration into food.
32105688	9	54	theme	antioxidant	1110:1120	arg1	activity					1122:1129	the challenging antioxidant activity	1094:1129	the challenging antioxidant activity in terms of DPPH and ABTS scavenging	1094:1166	In addition, CNW (0.5%)-SA(3%)-CuO NPs (5 mM) film showed the challenging antioxidant activity in terms of DPPH and ABTS scavenging.
32105688	6	55	theme	zone	850:853	arg1	terms					834:838	terms	834:838	terms of higher zone of inhibition against S. aureus (27.49 ± 0.91 mm), E. coli (12.12 ± 0.58 mm), Salmonella sp	834:945	The film composed with CNW (0.5%)-SA (3%)-CuNPs (5 mM) exhibited promising antibacterial activity against several pathogens in terms of higher zone of inhibition against S. aureus (27.49 ± 0.91 mm), E. coli (12.12 ± 0.58 mm), Salmonella sp.
32105688	6	56	theme	higher	843:848	arg1	zone					850:853	higher zone	843:853	higher zone of inhibition against S. aureus (27.49 ± 0.91 mm), E. coli (12.12 ± 0.58 mm), Salmonella sp	843:945	The film composed with CNW (0.5%)-SA (3%)-CuNPs (5 mM) exhibited promising antibacterial activity against several pathogens in terms of higher zone of inhibition against S. aureus (27.49 ± 0.91 mm), E. coli (12.12 ± 0.58 mm), Salmonella sp.
32105688	6	57	theme	inhibition	858:867	arg1	zone					850:853	higher zone	843:853	higher zone of inhibition against S. aureus (27.49 ± 0.91 mm), E. coli (12.12 ± 0.58 mm), Salmonella sp	843:945	The film composed with CNW (0.5%)-SA (3%)-CuNPs (5 mM) exhibited promising antibacterial activity against several pathogens in terms of higher zone of inhibition against S. aureus (27.49 ± 0.91 mm), E. coli (12.12 ± 0.58 mm), Salmonella sp.
32105688	9	58	theme	scavenging	1157:1166	arg1	terms					1134:1138	terms	1134:1138	terms of DPPH and ABTS scavenging	1134:1166	In addition, CNW (0.5%)-SA(3%)-CuO NPs (5 mM) film showed the challenging antioxidant activity in terms of DPPH and ABTS scavenging.
32105688	11	59	theme	packaging	1421:1429	arg1	FPS					1439:1441	FPS	1439:1441	FPS	1439:1441	Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
32105688	11	59	theme	packaging	1421:1429	arg1	system					1431:1436	an active food packaging system	1406:1436	an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging	1406:1534	Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
32105688	11	59	theme	packaging	1421:1429	arg1	APF					1338:1340	APF	1338:1340	APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM)	1338:1388	Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
32105688	11	60	theme	active	1409:1414	arg1	FPS					1439:1441	FPS	1439:1441	FPS	1439:1441	Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
32105688	11	60	theme	active	1409:1414	arg1	system					1431:1436	an active food packaging system	1406:1436	an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging	1406:1534	Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
32105688	11	60	theme	active	1409:1414	arg1	APF					1338:1340	APF	1338:1340	APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM)	1338:1388	Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
32105688	10	61	theme	fresh	1303:1307	arg1	pepper					1313:1318	fresh cut pepper	1303:1318	fresh cut pepper (FCP)	1303:1324	Also, the optimized composition of film composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) was preventing the microbial contamination in fresh cut pepper (FCP).
32105688	10	61	theme	fresh	1303:1307	arg1	FCP					1321:1323	FCP	1321:1323	FCP	1321:1323	Also, the optimized composition of film composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) was preventing the microbial contamination in fresh cut pepper (FCP).
32105688	9	62	theme	film	1082:1085	arg1	-SA					1059:1061	CNW (0.5%)-SA	1049:1061	CNW (0.5%)-SA(3%)-CuO NPs (5 mM) film	1049:1085	In addition, CNW (0.5%)-SA(3%)-CuO NPs (5 mM) film showed the challenging antioxidant activity in terms of DPPH and ABTS scavenging.
32105688	9	62	theme	film	1082:1085	arg1	%					1064:1064	3%	1063:1064	3%	1063:1064	In addition, CNW (0.5%)-SA(3%)-CuO NPs (5 mM) film showed the challenging antioxidant activity in terms of DPPH and ABTS scavenging.
32105688	11	63	theme	CNW	1356:1358	arg1	-SA					1366:1368	CNW (0.5%)-SA	1356:1368	CNW (0.5%)-SA (3%)-CuO NPs (5 mM)	1356:1388	Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
32105688	11	63	theme	CNW	1356:1358	arg1	%					1372:1372	3%	1371:1372	3%	1371:1372	Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
32105688	3	64	theme	film	467:470	arg1	properties					449:458	barrier properties	441:458	barrier properties of the film	441:470	The CNW improves barrier properties of the film to limit moisture penetration into food.
32105688	1	65	theme	different	232:240	arg1	combination					242:252	the different combination	228:252	the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs)	228:360	The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs).
32105688	0	66	theme	cellulose	67:75	arg1	nanoparticles					111:123	cellulose, sodium alginate and copper oxide nanoparticles	67:123	cellulose, sodium alginate and copper oxide nanoparticles for food packaging application	67:154	Physical and bioactivities of biopolymeric films incorporated with cellulose, sodium alginate and copper oxide nanoparticles for food packaging application.
32105688	11	67	theme	-CuO	1374:1377	arg1	NPs					1379:1381	CNW (0.5%)-SA (3%)-CuO NPs	1356:1381	CNW (0.5%)-SA (3%)-CuO NPs (5 mM)	1356:1388	Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
32105688	11	67	theme	-CuO	1374:1377	arg1	5 mM					1384:1387	5 mM	1384:1387	5 mM	1384:1387	Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
32105688	11	68	from	utility	1452:1458	arg1	industry					1468:1475	food industry	1463:1475	food industry	1463:1475	Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
32767501	6	0	theme	Ac-PRXs	859:865	arg1	temperature-responsivities					829:854	These temperature-responsivities	823:854	These temperature-responsivities of Ac-PRXs	823:865	These temperature-responsivities of Ac-PRXs are observed for other PRXs with different axle polymer molecular weights and compositions.
32767501	0	1	theme	Formation	113:121	arg1	Properties					123:132	Coacervate Formation Properties	102:132	Coacervate Formation Properties	102:132	Acetylation of Cyclodextrin-Threaded Polyrotaxanes Yields Temperature-Responsive Phase Transition and Coacervate Formation Properties.
32767501	1	2	theme	biomedical	226:235	arg1	fields					237:242	biomedical fields	226:242	biomedical fields	226:242	Stimuli-responsive materials have received considerable attention for their application in biomedical fields.
32767501	7	3	theme	chemical	1014:1021	arg1	acetylation					977:987	the acetylation	973:987	the acetylation of PRXs	973:995	Consequently, the acetylation of PRXs is an attractive chemical modification for yielding temperature-responsivities, and Ac-PRXs can be applied as temperature-responsive supramolecular materials.
32767501	7	3	theme	chemical	1014:1021	arg1	modification					1023:1034	an attractive chemical modification	1000:1034	an attractive chemical modification for yielding temperature-responsivities	1000:1074	Consequently, the acetylation of PRXs is an attractive chemical modification for yielding temperature-responsivities, and Ac-PRXs can be applied as temperature-responsive supramolecular materials.
32767501	4	4	theme	hydrophobic	692:702	arg1	interactions					704:715	hydrophobic interactions	692:715	hydrophobic interactions	692:715	Upon increasing the temperature, Ac-PRXs are dehydrated and acetyl groups are subsequently associated through hydrophobic interactions.
32767501	5	5	theme	≈2-3	789:792	arg1	µm					794:795	≈2-3 µm	789:795	≈2-3 µm	789:795	Interestingly, the Ac-PRXs form coacervate droplets with a diameter of ≈2-3 µm above their cloud points.
32767501	2	6	from	polymer	410:416	arg1	solution					429:436	aqueous solution	421:436	aqueous solution	421:436	Herein, the temperature-dependent phase transition behavior of acetylated polyrotaxanes (Ac-PRXs) consisting of acetylated cyclodextrins threaded onto a linear axle polymer in aqueous solution is investigated.
32767501	5	7	theme	µm	794:795	arg1	diameter					777:784	a diameter	775:784	a diameter of ≈2-3 µm	775:795	Interestingly, the Ac-PRXs form coacervate droplets with a diameter of ≈2-3 µm above their cloud points.
32767501	5	8	theme	Ac-PRXs	737:743	arg1	coacervate					750:759	the Ac-PRXs form coacervate	733:759	the Ac-PRXs form coacervate	733:759	Interestingly, the Ac-PRXs form coacervate droplets with a diameter of ≈2-3 µm above their cloud points.
32767501	1	9	from	application	211:221	arg1	fields					237:242	biomedical fields	226:242	biomedical fields	226:242	Stimuli-responsive materials have received considerable attention for their application in biomedical fields.
32767501	2	10	theme	acetylated	357:366	arg1	cyclodextrins					368:380	acetylated cyclodextrins	357:380	acetylated cyclodextrins threaded onto a linear axle polymer in aqueous solution	357:436	Herein, the temperature-dependent phase transition behavior of acetylated polyrotaxanes (Ac-PRXs) consisting of acetylated cyclodextrins threaded onto a linear axle polymer in aqueous solution is investigated.
32767501	7	11	theme	attractive	1003:1012	arg1	acetylation					977:987	the acetylation	973:987	the acetylation of PRXs	973:995	Consequently, the acetylation of PRXs is an attractive chemical modification for yielding temperature-responsivities, and Ac-PRXs can be applied as temperature-responsive supramolecular materials.
32767501	7	11	theme	attractive	1003:1012	arg1	modification					1023:1034	an attractive chemical modification	1000:1034	an attractive chemical modification for yielding temperature-responsivities	1000:1074	Consequently, the acetylation of PRXs is an attractive chemical modification for yielding temperature-responsivities, and Ac-PRXs can be applied as temperature-responsive supramolecular materials.
32767501	3	12	theme	temperature-dependent	496:516	arg1	changes					532:538	temperature-dependent transmittance changes	496:538	temperature-dependent transmittance changes	496:538	The aqueous solutions of Ac-PRXs exhibit temperature-dependent transmittance changes when the degree of acetylation is 30-40%.
32767501	3	13	theme	transmittance	518:530	arg1	changes					532:538	temperature-dependent transmittance changes	496:538	temperature-dependent transmittance changes	496:538	The aqueous solutions of Ac-PRXs exhibit temperature-dependent transmittance changes when the degree of acetylation is 30-40%.
32767501	7	14	theme	PRXs	992:995	arg1	acetylation					977:987	the acetylation	973:987	the acetylation of PRXs	973:995	Consequently, the acetylation of PRXs is an attractive chemical modification for yielding temperature-responsivities, and Ac-PRXs can be applied as temperature-responsive supramolecular materials.
32767501	7	14	theme	PRXs	992:995	arg1	modification					1023:1034	an attractive chemical modification	1000:1034	an attractive chemical modification for yielding temperature-responsivities	1000:1074	Consequently, the acetylation of PRXs is an attractive chemical modification for yielding temperature-responsivities, and Ac-PRXs can be applied as temperature-responsive supramolecular materials.
32767501	7	15	theme	temperature-responsive	1107:1128	arg1	materials					1145:1153	temperature-responsive supramolecular materials	1107:1153	temperature-responsive supramolecular materials	1107:1153	Consequently, the acetylation of PRXs is an attractive chemical modification for yielding temperature-responsivities, and Ac-PRXs can be applied as temperature-responsive supramolecular materials.
32767501	7	15	theme	temperature-responsive	1107:1128	arg1	Ac-PRXs					1081:1087	Ac-PRXs	1081:1087	Ac-PRXs	1081:1087	Consequently, the acetylation of PRXs is an attractive chemical modification for yielding temperature-responsivities, and Ac-PRXs can be applied as temperature-responsive supramolecular materials.
32767501	3	16	theme	aqueous	459:465	arg1	solutions					467:475	The aqueous solutions	455:475	The aqueous solutions of Ac-PRXs	455:486	The aqueous solutions of Ac-PRXs exhibit temperature-dependent transmittance changes when the degree of acetylation is 30-40%.
32767501	6	17	theme	molecular	923:931	arg1	weights					933:939	different axle polymer molecular weights	900:939	different axle polymer molecular weights	900:939	These temperature-responsivities of Ac-PRXs are observed for other PRXs with different axle polymer molecular weights and compositions.
32767501	2	18	theme	axle	405:408	arg1	polymer					410:416	a linear axle polymer	396:416	a linear axle polymer in aqueous solution	396:436	Herein, the temperature-dependent phase transition behavior of acetylated polyrotaxanes (Ac-PRXs) consisting of acetylated cyclodextrins threaded onto a linear axle polymer in aqueous solution is investigated.
32767501	3	19	theme	Ac-PRXs	480:486	arg1	solutions					467:475	The aqueous solutions	455:475	The aqueous solutions of Ac-PRXs	455:486	The aqueous solutions of Ac-PRXs exhibit temperature-dependent transmittance changes when the degree of acetylation is 30-40%.
32767501	6	20	theme	polymer	915:921	arg1	weights					933:939	different axle polymer molecular weights	900:939	different axle polymer molecular weights	900:939	These temperature-responsivities of Ac-PRXs are observed for other PRXs with different axle polymer molecular weights and compositions.
32767501	2	21	theme	linear	398:403	arg1	polymer					410:416	a linear axle polymer	396:416	a linear axle polymer in aqueous solution	396:436	Herein, the temperature-dependent phase transition behavior of acetylated polyrotaxanes (Ac-PRXs) consisting of acetylated cyclodextrins threaded onto a linear axle polymer in aqueous solution is investigated.
32767501	0	22	theme	Polyrotaxanes	37:49	arg1	Acetylation					0:10	Acetylation	0:10	Acetylation of Cyclodextrin-Threaded Polyrotaxanes	0:49	Acetylation of Cyclodextrin-Threaded Polyrotaxanes Yields Temperature-Responsive Phase Transition and Coacervate Formation Properties.
32767501	5	23	theme	form	745:748	arg1	coacervate					750:759	the Ac-PRXs form coacervate	733:759	the Ac-PRXs form coacervate	733:759	Interestingly, the Ac-PRXs form coacervate droplets with a diameter of ≈2-3 µm above their cloud points.
32767501	1	24	theme	Stimuli-responsive	135:152	arg1	materials					154:162	Stimuli-responsive materials	135:162	Stimuli-responsive materials	135:162	Stimuli-responsive materials have received considerable attention for their application in biomedical fields.
32767501	7	25	theme	supramolecular	1130:1143	arg1	materials					1145:1153	temperature-responsive supramolecular materials	1107:1153	temperature-responsive supramolecular materials	1107:1153	Consequently, the acetylation of PRXs is an attractive chemical modification for yielding temperature-responsivities, and Ac-PRXs can be applied as temperature-responsive supramolecular materials.
32767501	7	25	theme	supramolecular	1130:1143	arg1	Ac-PRXs					1081:1087	Ac-PRXs	1081:1087	Ac-PRXs	1081:1087	Consequently, the acetylation of PRXs is an attractive chemical modification for yielding temperature-responsivities, and Ac-PRXs can be applied as temperature-responsive supramolecular materials.
32767501	0	26	theme	Temperature-Responsive	58:79	arg1	Transition					87:96	Temperature-Responsive Phase Transition	58:96	Temperature-Responsive Phase Transition	58:96	Acetylation of Cyclodextrin-Threaded Polyrotaxanes Yields Temperature-Responsive Phase Transition and Coacervate Formation Properties.
32767501	2	27	theme	aqueous	421:427	arg1	solution					429:436	aqueous solution	421:436	aqueous solution	421:436	Herein, the temperature-dependent phase transition behavior of acetylated polyrotaxanes (Ac-PRXs) consisting of acetylated cyclodextrins threaded onto a linear axle polymer in aqueous solution is investigated.
32767501	2	28	theme	polyrotaxanes	319:331	arg1	behavior					296:303	the temperature-dependent phase transition behavior	253:303	the temperature-dependent phase transition behavior of acetylated polyrotaxanes (Ac-PRXs) consisting of acetylated cyclodextrins threaded onto a linear axle polymer in aqueous solution	253:436	Herein, the temperature-dependent phase transition behavior of acetylated polyrotaxanes (Ac-PRXs) consisting of acetylated cyclodextrins threaded onto a linear axle polymer in aqueous solution is investigated.
32767501	3	29	theme	acetylation	559:569	arg1	degree					549:554	the degree	545:554	the degree of acetylation	545:569	The aqueous solutions of Ac-PRXs exhibit temperature-dependent transmittance changes when the degree of acetylation is 30-40%.
32767501	3	29	theme	acetylation	559:569	arg1	%					579:579	30-40%	574:579	30-40%	574:579	The aqueous solutions of Ac-PRXs exhibit temperature-dependent transmittance changes when the degree of acetylation is 30-40%.
32767501	2	30	theme	acetylated	308:317	arg1	Ac-PRXs					334:340	Ac-PRXs	334:340	Ac-PRXs	334:340	Herein, the temperature-dependent phase transition behavior of acetylated polyrotaxanes (Ac-PRXs) consisting of acetylated cyclodextrins threaded onto a linear axle polymer in aqueous solution is investigated.
32767501	2	30	theme	acetylated	308:317	arg1	polyrotaxanes					319:331	acetylated polyrotaxanes	308:331	acetylated polyrotaxanes (Ac-PRXs) consisting of acetylated cyclodextrins threaded onto a linear axle polymer in aqueous solution	308:436	Herein, the temperature-dependent phase transition behavior of acetylated polyrotaxanes (Ac-PRXs) consisting of acetylated cyclodextrins threaded onto a linear axle polymer in aqueous solution is investigated.
32767501	6	31	theme	different	900:908	arg1	weights					933:939	different axle polymer molecular weights	900:939	different axle polymer molecular weights	900:939	These temperature-responsivities of Ac-PRXs are observed for other PRXs with different axle polymer molecular weights and compositions.
32767501	6	32	theme	axle	910:913	arg1	weights					933:939	different axle polymer molecular weights	900:939	different axle polymer molecular weights	900:939	These temperature-responsivities of Ac-PRXs are observed for other PRXs with different axle polymer molecular weights and compositions.
32767501	0	33	theme	Phase	81:85	arg1	Transition					87:96	Temperature-Responsive Phase Transition	58:96	Temperature-Responsive Phase Transition	58:96	Acetylation of Cyclodextrin-Threaded Polyrotaxanes Yields Temperature-Responsive Phase Transition and Coacervate Formation Properties.
32767501	2	34	theme	transition	285:294	arg1	behavior					296:303	the temperature-dependent phase transition behavior	253:303	the temperature-dependent phase transition behavior of acetylated polyrotaxanes (Ac-PRXs) consisting of acetylated cyclodextrins threaded onto a linear axle polymer in aqueous solution	253:436	Herein, the temperature-dependent phase transition behavior of acetylated polyrotaxanes (Ac-PRXs) consisting of acetylated cyclodextrins threaded onto a linear axle polymer in aqueous solution is investigated.
32767501	1	35	theme	considerable	178:189	arg1	attention					191:199	considerable attention	178:199	considerable attention	178:199	Stimuli-responsive materials have received considerable attention for their application in biomedical fields.
32767501	4	36	theme	acetyl	642:647	arg1	groups					649:654	acetyl groups	642:654	acetyl groups	642:654	Upon increasing the temperature, Ac-PRXs are dehydrated and acetyl groups are subsequently associated through hydrophobic interactions.
32767501	5	37	theme	cloud	809:813	arg1	points					815:820	their cloud points	803:820	their cloud points	803:820	Interestingly, the Ac-PRXs form coacervate droplets with a diameter of ≈2-3 µm above their cloud points.
32767501	2	38	theme	phase	279:283	arg1	behavior					296:303	the temperature-dependent phase transition behavior	253:303	the temperature-dependent phase transition behavior of acetylated polyrotaxanes (Ac-PRXs) consisting of acetylated cyclodextrins threaded onto a linear axle polymer in aqueous solution	253:436	Herein, the temperature-dependent phase transition behavior of acetylated polyrotaxanes (Ac-PRXs) consisting of acetylated cyclodextrins threaded onto a linear axle polymer in aqueous solution is investigated.
32767501	6	39	theme	other	884:888	arg1	PRXs					890:893	other PRXs	884:893	other PRXs with different axle polymer molecular weights and compositions	884:956	These temperature-responsivities of Ac-PRXs are observed for other PRXs with different axle polymer molecular weights and compositions.
32767501	6	40	with	PRXs	890:893	arg1	compositions					945:956	compositions	945:956	compositions	945:956	These temperature-responsivities of Ac-PRXs are observed for other PRXs with different axle polymer molecular weights and compositions.
32767501	6	40	with	PRXs	890:893	arg1	weights					933:939	different axle polymer molecular weights	900:939	different axle polymer molecular weights	900:939	These temperature-responsivities of Ac-PRXs are observed for other PRXs with different axle polymer molecular weights and compositions.
32767501	2	41	theme	temperature-dependent	257:277	arg1	behavior					296:303	the temperature-dependent phase transition behavior	253:303	the temperature-dependent phase transition behavior of acetylated polyrotaxanes (Ac-PRXs) consisting of acetylated cyclodextrins threaded onto a linear axle polymer in aqueous solution	253:436	Herein, the temperature-dependent phase transition behavior of acetylated polyrotaxanes (Ac-PRXs) consisting of acetylated cyclodextrins threaded onto a linear axle polymer in aqueous solution is investigated.
32767501	0	42	theme	Coacervate	102:111	arg1	Properties					123:132	Coacervate Formation Properties	102:132	Coacervate Formation Properties	102:132	Acetylation of Cyclodextrin-Threaded Polyrotaxanes Yields Temperature-Responsive Phase Transition and Coacervate Formation Properties.
32767501	0	43	theme	Cyclodextrin-Threaded	15:35	arg1	Polyrotaxanes					37:49	Cyclodextrin-Threaded Polyrotaxanes	15:49	Cyclodextrin-Threaded Polyrotaxanes	15:49	Acetylation of Cyclodextrin-Threaded Polyrotaxanes Yields Temperature-Responsive Phase Transition and Coacervate Formation Properties.
33570460	0	0	theme	Standardized	94:105	arg1	Composition					120:130	Standardized Bioflavonoid Composition	94:130	Standardized Bioflavonoid Composition	94:130	Antinociceptive and Anti-Inflammatory Properties of Cannabidiol Alone and in Combination with Standardized Bioflavonoid Composition.
33570460	6	1	theme	CBD	1113:1115	arg1	activity					1101:1108	the antinociceptive and anti-inflammatory activity	1059:1108	the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions	1059:1184	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	6	2	theme	paw	1016:1018	arg1	model					1026:1030	carrageenan-induced rat paw edema model	992:1030	carrageenan-induced rat paw edema model	992:1030	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	5	3	theme	preclinical	806:816	arg1	models					818:823	preclinical models	806:823	preclinical models of arthritis	806:836	It has shown promise in reducing pain and inflammation in preclinical models of arthritis.
33570460	6	4	theme	plate	939:943	arg1	test					945:948	the hot plate test	931:948	the hot plate test	931:948	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	6	5	theme	employed	861:868	arg1	models					915:920	widely employed inflammatory and noninflammatory animal pain models	854:920	widely employed inflammatory and noninflammatory animal pain models	854:920	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	6	6	used	utilized	1038:1045	arg2	models					915:920	widely employed inflammatory and noninflammatory animal pain models	854:920	widely employed inflammatory and noninflammatory animal pain models	854:920	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	6	6	used	utilized	1038:1045	arg2	model					1026:1030	carrageenan-induced rat paw edema model	992:1030	carrageenan-induced rat paw edema model	992:1030	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	6	6	used	utilized	1038:1045	arg2	test					945:948	the hot plate test	931:948	the hot plate test	931:948	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	6	6	used	utilized	1038:1045	arg2	test					981:984	writhing test	972:984	writhing test	972:984	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	6	6	used	utilized	1038:1045	arg2	model					965:969	visceral pain model	951:969	visceral pain model (writhing test)	951:985	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	4	7	theme	pharmacological	723:737	arg1	actions					739:745	its pharmacological actions	719:745	its pharmacological actions	719:745	Recently, cannabidiol (CBD), the major nonpsychoactive component of the hemp plant, has gained renewed global attention for its pharmacological actions.
33570460	6	8	theme	rat	1012:1014	arg1	model					1026:1030	carrageenan-induced rat paw edema model	992:1030	carrageenan-induced rat paw edema model	992:1030	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	3	9	theme	botanical	403:411	arg1	extracts					413:420	traditionally used botanical extracts	384:420	traditionally used botanical extracts	384:420	We believe that a combination of traditionally used botanical extracts, which have diverse active components that target multiple inflammatory pathways, may provide a safe and efficacious alternative to address the multifactorial nature of OA.
33570460	6	10	theme	hot	935:937	arg1	test					945:948	the hot plate test	931:948	the hot plate test	931:948	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	2	11	theme	side	337:340	arg1	effects					342:348	associated side effects	326:348	associated side effects	326:348	Long-term application of first-line treatments, such as nonsteroidal anti-inflammatory drugs, is limited due to associated side effects.
33570460	6	12	theme	bioflavonoid	1160:1171	arg1	compositions					1173:1184	standardized bioflavonoid compositions	1147:1184	standardized bioflavonoid compositions	1147:1184	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	4	13	theme	major	628:632	arg1	component					650:658	the major nonpsychoactive component	624:658	the major nonpsychoactive component of the hemp plant	624:676	Recently, cannabidiol (CBD), the major nonpsychoactive component of the hemp plant, has gained renewed global attention for its pharmacological actions.
33570460	4	13	theme	major	628:632	arg1	cannabidiol					605:615	cannabidiol	605:615	cannabidiol (CBD)	605:621	Recently, cannabidiol (CBD), the major nonpsychoactive component of the hemp plant, has gained renewed global attention for its pharmacological actions.
33570460	0	14	theme	Bioflavonoid	107:118	arg1	Composition					120:130	Standardized Bioflavonoid Composition	94:130	Standardized Bioflavonoid Composition	94:130	Antinociceptive and Anti-Inflammatory Properties of Cannabidiol Alone and in Combination with Standardized Bioflavonoid Composition.
33570460	2	15	theme	associated	326:335	arg1	effects					342:348	associated side effects	326:348	associated side effects	326:348	Long-term application of first-line treatments, such as nonsteroidal anti-inflammatory drugs, is limited due to associated side effects.
33570460	0	16	with	Combination	77:87	arg1	Composition					120:130	Standardized Bioflavonoid Composition	94:130	Standardized Bioflavonoid Composition	94:130	Antinociceptive and Anti-Inflammatory Properties of Cannabidiol Alone and in Combination with Standardized Bioflavonoid Composition.
33570460	0	17	theme	Antinociceptive	0:14	arg1	Properties					38:47	Antinociceptive and Anti-Inflammatory Properties	0:47	Antinociceptive and Anti-Inflammatory Properties of Cannabidiol Alone and in Combination with Standardized Bioflavonoid Composition	0:130	Antinociceptive and Anti-Inflammatory Properties of Cannabidiol Alone and in Combination with Standardized Bioflavonoid Composition.
33570460	10	18	theme	similar	1688:1694	arg1	activities					1696:1705	similar activities	1688:1705	similar activities	1688:1705	Data from this study show that for clinically meaningful efficacy against OA, CBD may have to be delivered in higher dosage or formulated with other medicinal plants with similar activities.
33570460	9	19	theme	bioflavonoid	1490:1501	arg1	compositions					1503:1514	the bioflavonoid compositions	1486:1514	the bioflavonoid compositions	1486:1514	Enhanced performance in pain and inflammation reduction was observed when CBD was orally administered in complex with the bioflavonoid compositions.
33570460	4	20	theme	hemp	667:670	arg1	plant					672:676	the hemp plant	663:676	the hemp plant	663:676	Recently, cannabidiol (CBD), the major nonpsychoactive component of the hemp plant, has gained renewed global attention for its pharmacological actions.
33570460	4	21	theme	renewed	690:696	arg1	attention					705:713	renewed global attention	690:713	renewed global attention	690:713	Recently, cannabidiol (CBD), the major nonpsychoactive component of the hemp plant, has gained renewed global attention for its pharmacological actions.
33570460	3	22	theme	used	398:401	arg1	extracts					413:420	traditionally used botanical extracts	384:420	traditionally used botanical extracts	384:420	We believe that a combination of traditionally used botanical extracts, which have diverse active components that target multiple inflammatory pathways, may provide a safe and efficacious alternative to address the multifactorial nature of OA.
33570460	10	23	theme	higher	1627:1632	arg1	dosage					1634:1639	higher dosage	1627:1639	higher dosage	1627:1639	Data from this study show that for clinically meaningful efficacy against OA, CBD may have to be delivered in higher dosage or formulated with other medicinal plants with similar activities.
33570460	3	24	theme	OA	591:592	arg1	nature					581:586	the multifactorial nature	562:586	the multifactorial nature of OA	562:592	We believe that a combination of traditionally used botanical extracts, which have diverse active components that target multiple inflammatory pathways, may provide a safe and efficacious alternative to address the multifactorial nature of OA.
33570460	6	25	theme	anti-inflammatory	1083:1099	arg1	activity					1101:1108	the antinociceptive and anti-inflammatory activity	1059:1108	the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions	1059:1184	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	9	26	dep	pain	1392:1395	arg1	reduction					1414:1422	reduction	1414:1422	reduction	1414:1422	Enhanced performance in pain and inflammation reduction was observed when CBD was orally administered in complex with the bioflavonoid compositions.
33570460	10	27	with	plants	1676:1681	arg1	activities					1696:1705	similar activities	1688:1705	similar activities	1688:1705	Data from this study show that for clinically meaningful efficacy against OA, CBD may have to be delivered in higher dosage or formulated with other medicinal plants with similar activities.
33570460	10	28	theme	meaningful	1563:1572	arg1	efficacy					1574:1581	clinically meaningful efficacy	1552:1581	clinically meaningful efficacy against OA	1552:1592	Data from this study show that for clinically meaningful efficacy against OA, CBD may have to be delivered in higher dosage or formulated with other medicinal plants with similar activities.
33570460	3	29	theme	multiple	472:479	arg1	pathways					494:501	multiple inflammatory pathways	472:501	multiple inflammatory pathways	472:501	We believe that a combination of traditionally used botanical extracts, which have diverse active components that target multiple inflammatory pathways, may provide a safe and efficacious alternative to address the multifactorial nature of OA.
33570460	3	30	theme	diverse	434:440	arg1	components					449:458	diverse active components	434:458	diverse active components that target multiple inflammatory pathways	434:501	We believe that a combination of traditionally used botanical extracts, which have diverse active components that target multiple inflammatory pathways, may provide a safe and efficacious alternative to address the multifactorial nature of OA.
33570460	9	31	theme	Enhanced	1368:1375	arg1	performance					1377:1387	Enhanced performance	1368:1387	Enhanced performance in pain and inflammation reduction	1368:1422	Enhanced performance in pain and inflammation reduction was observed when CBD was orally administered in complex with the bioflavonoid compositions.
33570460	3	32	theme	inflammatory	481:492	arg1	pathways					494:501	multiple inflammatory pathways	472:501	multiple inflammatory pathways	472:501	We believe that a combination of traditionally used botanical extracts, which have diverse active components that target multiple inflammatory pathways, may provide a safe and efficacious alternative to address the multifactorial nature of OA.
33570460	8	33	theme	dose-correlated	1290:1304	arg1	inhibition					1338:1347	dose-correlated, statistically significant pain inhibition	1290:1347	dose-correlated, statistically significant pain inhibition	1290:1347	Administered alone, CBD produced dose-correlated, statistically significant pain inhibition in all the models.
33570460	3	34	contain	have	429:432	arg1	combination					369:379	a combination	367:379	a combination	367:379	We believe that a combination of traditionally used botanical extracts, which have diverse active components that target multiple inflammatory pathways, may provide a safe and efficacious alternative to address the multifactorial nature of OA.
33570460	3	34	contain	have	429:432	arg2	components					449:458	diverse active components	434:458	diverse active components that target multiple inflammatory pathways	434:501	We believe that a combination of traditionally used botanical extracts, which have diverse active components that target multiple inflammatory pathways, may provide a safe and efficacious alternative to address the multifactorial nature of OA.
33570460	4	35	theme	plant	672:676	arg1	component					650:658	the major nonpsychoactive component	624:658	the major nonpsychoactive component of the hemp plant	624:676	Recently, cannabidiol (CBD), the major nonpsychoactive component of the hemp plant, has gained renewed global attention for its pharmacological actions.
33570460	4	35	theme	plant	672:676	arg1	cannabidiol					605:615	cannabidiol	605:615	cannabidiol (CBD)	605:621	Recently, cannabidiol (CBD), the major nonpsychoactive component of the hemp plant, has gained renewed global attention for its pharmacological actions.
33570460	0	36	theme	Anti-Inflammatory	20:36	arg1	Properties					38:47	Antinociceptive and Anti-Inflammatory Properties	0:47	Antinociceptive and Anti-Inflammatory Properties of Cannabidiol Alone and in Combination with Standardized Bioflavonoid Composition	0:130	Antinociceptive and Anti-Inflammatory Properties of Cannabidiol Alone and in Combination with Standardized Bioflavonoid Composition.
33570460	1	37	theme	Symptom-alleviating	133:151	arg1	therapies					153:161	Symptom-alleviating therapies	133:161	Symptom-alleviating therapies for osteoarthritis (OA) management	133:196	Symptom-alleviating therapies for osteoarthritis (OA) management are inadequate.
33570460	3	38	theme	safe	518:521	arg1	alternative					539:549	a safe and efficacious alternative	516:549	a safe and efficacious alternative to address the multifactorial nature of OA	516:592	We believe that a combination of traditionally used botanical extracts, which have diverse active components that target multiple inflammatory pathways, may provide a safe and efficacious alternative to address the multifactorial nature of OA.
33570460	0	39	theme	Cannabidiol	52:62	arg1	Properties					38:47	Antinociceptive and Anti-Inflammatory Properties	0:47	Antinociceptive and Anti-Inflammatory Properties of Cannabidiol Alone and in Combination with Standardized Bioflavonoid Composition	0:130	Antinociceptive and Anti-Inflammatory Properties of Cannabidiol Alone and in Combination with Standardized Bioflavonoid Composition.
33570460	6	40	theme	writhing	972:979	arg1	model					965:969	visceral pain model	951:969	visceral pain model (writhing test)	951:985	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	6	40	theme	writhing	972:979	arg1	test					981:984	writhing test	972:984	writhing test	972:984	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	2	41	theme	Long-term	214:222	arg1	application					224:234	Long-term application	214:234	Long-term application of first-line treatments, such as nonsteroidal anti-inflammatory drugs,	214:306	Long-term application of first-line treatments, such as nonsteroidal anti-inflammatory drugs, is limited due to associated side effects.
33570460	2	42	theme	anti-inflammatory	283:299	arg1	drugs					301:305	nonsteroidal anti-inflammatory drugs	270:305	nonsteroidal anti-inflammatory drugs	270:305	Long-term application of first-line treatments, such as nonsteroidal anti-inflammatory drugs, is limited due to associated side effects.
33570460	4	43	theme	global	698:703	arg1	attention					705:713	renewed global attention	690:713	renewed global attention	690:713	Recently, cannabidiol (CBD), the major nonpsychoactive component of the hemp plant, has gained renewed global attention for its pharmacological actions.
33570460	10	44	theme	medicinal	1666:1674	arg1	plants					1676:1681	other medicinal plants	1660:1681	other medicinal plants with similar activities	1660:1705	Data from this study show that for clinically meaningful efficacy against OA, CBD may have to be delivered in higher dosage or formulated with other medicinal plants with similar activities.
33570460	9	45	with	complex	1473:1479	arg1	compositions					1503:1514	the bioflavonoid compositions	1486:1514	the bioflavonoid compositions	1486:1514	Enhanced performance in pain and inflammation reduction was observed when CBD was orally administered in complex with the bioflavonoid compositions.
33570460	6	46	theme	animal	903:908	arg1	models					915:920	widely employed inflammatory and noninflammatory animal pain models	854:920	widely employed inflammatory and noninflammatory animal pain models	854:920	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	2	47	theme	nonsteroidal	270:281	arg1	drugs					301:305	nonsteroidal anti-inflammatory drugs	270:305	nonsteroidal anti-inflammatory drugs	270:305	Long-term application of first-line treatments, such as nonsteroidal anti-inflammatory drugs, is limited due to associated side effects.
33570460	10	48	theme	other	1660:1664	arg1	plants					1676:1681	other medicinal plants	1660:1681	other medicinal plants with similar activities	1660:1705	Data from this study show that for clinically meaningful efficacy against OA, CBD may have to be delivered in higher dosage or formulated with other medicinal plants with similar activities.
33570460	3	49	theme	active	442:447	arg1	components					449:458	diverse active components	434:458	diverse active components that target multiple inflammatory pathways	434:501	We believe that a combination of traditionally used botanical extracts, which have diverse active components that target multiple inflammatory pathways, may provide a safe and efficacious alternative to address the multifactorial nature of OA.
33570460	5	50	from	pain	781:784	arg1	models					818:823	preclinical models	806:823	preclinical models of arthritis	806:836	It has shown promise in reducing pain and inflammation in preclinical models of arthritis.
33570460	6	51	theme	noninflammatory	887:901	arg1	models					915:920	widely employed inflammatory and noninflammatory animal pain models	854:920	widely employed inflammatory and noninflammatory animal pain models	854:920	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	9	52	from	performance	1377:1387	arg1	inflammation					1401:1412	inflammation	1401:1412	inflammation	1401:1412	Enhanced performance in pain and inflammation reduction was observed when CBD was orally administered in complex with the bioflavonoid compositions.
33570460	9	52	from	performance	1377:1387	arg1	pain					1392:1395	pain	1392:1395	pain	1392:1395	Enhanced performance in pain and inflammation reduction was observed when CBD was orally administered in complex with the bioflavonoid compositions.
33570460	6	53	theme	standardized	1147:1158	arg1	compositions					1173:1184	standardized bioflavonoid compositions	1147:1184	standardized bioflavonoid compositions	1147:1184	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	6	54	theme	pain	960:963	arg1	model					965:969	visceral pain model	951:969	visceral pain model (writhing test)	951:985	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	6	54	theme	pain	960:963	arg1	test					981:984	writhing test	972:984	writhing test	972:984	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	6	55	theme	inflammatory	870:881	arg1	models					915:920	widely employed inflammatory and noninflammatory animal pain models	854:920	widely employed inflammatory and noninflammatory animal pain models	854:920	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	6	56	theme	visceral	951:958	arg1	model					965:969	visceral pain model	951:969	visceral pain model (writhing test)	951:985	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	6	56	theme	visceral	951:958	arg1	test					981:984	writhing test	972:984	writhing test	972:984	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	2	57	theme	treatments	250:259	arg1	application					224:234	Long-term application	214:234	Long-term application of first-line treatments, such as nonsteroidal anti-inflammatory drugs,	214:306	Long-term application of first-line treatments, such as nonsteroidal anti-inflammatory drugs, is limited due to associated side effects.
33570460	8	58	dep	dose-correlated	1290:1304	arg1	significant					1321:1331	significant	1321:1331	significant	1321:1331	Administered alone, CBD produced dose-correlated, statistically significant pain inhibition in all the models.
33570460	1	59	theme	osteoarthritis	167:180	arg1	management					187:196	osteoarthritis (OA) management	167:196	osteoarthritis (OA) management	167:196	Symptom-alleviating therapies for osteoarthritis (OA) management are inadequate.
33570460	6	60	theme	carrageenan-induced	992:1010	arg1	model					1026:1030	carrageenan-induced rat paw edema model	992:1030	carrageenan-induced rat paw edema model	992:1030	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	2	61	theme	first-line	239:248	arg1	treatments					250:259	first-line treatments	239:259	first-line treatments	239:259	Long-term application of first-line treatments, such as nonsteroidal anti-inflammatory drugs, is limited due to associated side effects.
33570460	2	61	theme	first-line	239:248	arg1	drugs					301:305	nonsteroidal anti-inflammatory drugs	270:305	nonsteroidal anti-inflammatory drugs	270:305	Long-term application of first-line treatments, such as nonsteroidal anti-inflammatory drugs, is limited due to associated side effects.
33570460	6	62	theme	antinociceptive	1063:1077	arg1	activity					1101:1108	the antinociceptive and anti-inflammatory activity	1059:1108	the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions	1059:1184	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	3	63	theme	efficacious	527:537	arg1	alternative					539:549	a safe and efficacious alternative	516:549	a safe and efficacious alternative to address the multifactorial nature of OA	516:592	We believe that a combination of traditionally used botanical extracts, which have diverse active components that target multiple inflammatory pathways, may provide a safe and efficacious alternative to address the multifactorial nature of OA.
33570460	8	64	theme	pain	1333:1336	arg1	inhibition					1338:1347	dose-correlated, statistically significant pain inhibition	1290:1347	dose-correlated, statistically significant pain inhibition	1290:1347	Administered alone, CBD produced dose-correlated, statistically significant pain inhibition in all the models.
33570460	4	65	theme	nonpsychoactive	634:648	arg1	component					650:658	the major nonpsychoactive component	624:658	the major nonpsychoactive component of the hemp plant	624:676	Recently, cannabidiol (CBD), the major nonpsychoactive component of the hemp plant, has gained renewed global attention for its pharmacological actions.
33570460	4	65	theme	nonpsychoactive	634:648	arg1	cannabidiol					605:615	cannabidiol	605:615	cannabidiol (CBD)	605:621	Recently, cannabidiol (CBD), the major nonpsychoactive component of the hemp plant, has gained renewed global attention for its pharmacological actions.
33570460	6	66	theme	pain	910:913	arg1	models					915:920	widely employed inflammatory and noninflammatory animal pain models	854:920	widely employed inflammatory and noninflammatory animal pain models	854:920	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	5	67	from	inflammation	790:801	arg1	models					818:823	preclinical models	806:823	preclinical models of arthritis	806:836	It has shown promise in reducing pain and inflammation in preclinical models of arthritis.
33570460	3	68	theme	multifactorial	566:579	arg1	nature					581:586	the multifactorial nature	562:586	the multifactorial nature of OA	562:592	We believe that a combination of traditionally used botanical extracts, which have diverse active components that target multiple inflammatory pathways, may provide a safe and efficacious alternative to address the multifactorial nature of OA.
33570460	10	69	from	study	1532:1536	arg1	Data					1517:1520	Data	1517:1520	Data from this study	1517:1536	Data from this study show that for clinically meaningful efficacy against OA, CBD may have to be delivered in higher dosage or formulated with other medicinal plants with similar activities.
33570460	5	70	theme	arthritis	828:836	arg1	models					818:823	preclinical models	806:823	preclinical models of arthritis	806:836	It has shown promise in reducing pain and inflammation in preclinical models of arthritis.
33570460	6	71	with	combination	1130:1140	arg1	compositions					1173:1184	standardized bioflavonoid compositions	1147:1184	standardized bioflavonoid compositions	1147:1184	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	6	72	theme	edema	1020:1024	arg1	model					1026:1030	carrageenan-induced rat paw edema model	992:1030	carrageenan-induced rat paw edema model	992:1030	In this study, widely employed inflammatory and noninflammatory animal pain models, such as the hot plate test, visceral pain model (writhing test), and carrageenan-induced rat paw edema model, were utilized to evaluate the antinociceptive and anti-inflammatory activity of CBD alone and in combination with standardized bioflavonoid compositions.
33570460	3	73	theme	extracts	413:420	arg1	combination					369:379	a combination	367:379	a combination	367:379	We believe that a combination of traditionally used botanical extracts, which have diverse active components that target multiple inflammatory pathways, may provide a safe and efficacious alternative to address the multifactorial nature of OA.
32553494	0	0	theme	water	109:113	arg1	solubility					115:124	the water solubility	105:124	the water solubility of tocopherol	105:138	Hyaluronic-acid-based β-cyclodextrin grafted copolymers as biocompatible supramolecular hosts to enhance the water solubility of tocopherol.
32553494	2	1	theme	varying	466:472	arg1	ratios					482:487	varying HA:β-CD ratios	466:487	varying HA:β-CD ratios	466:487	Herein, different copolymers were prepared with varying HA:β-CD ratios and characterized.
32553494	1	2	theme	drug	396:399	arg1	system					410:415	a model drug delivery system	388:415	a model drug delivery system	388:415	Hyaluronic acid (HA), a common biopolymer found in the extracellular fluid, was grafted with β-cyclodextrin (β-CD) to form a composite polymer that could form inclusion complexes with tocopherol (VE), enhancing its water-solubility and serving as a model drug delivery system.
32553494	1	3	theme	common	165:170	arg1	biopolymer					172:181	a common biopolymer	163:181	a common biopolymer found in the extracellular fluid	163:214	Hyaluronic acid (HA), a common biopolymer found in the extracellular fluid, was grafted with β-cyclodextrin (β-CD) to form a composite polymer that could form inclusion complexes with tocopherol (VE), enhancing its water-solubility and serving as a model drug delivery system.
32553494	1	3	theme	common	165:170	arg1	acid					152:155	Hyaluronic acid	141:155	Hyaluronic acid (HA)	141:160	Hyaluronic acid (HA), a common biopolymer found in the extracellular fluid, was grafted with β-cyclodextrin (β-CD) to form a composite polymer that could form inclusion complexes with tocopherol (VE), enhancing its water-solubility and serving as a model drug delivery system.
32553494	3	4	theme	loading	511:517	arg1	capacity					519:526	VE loading capacity	508:526	VE loading capacity	508:526	VE loading capacity was directly correlated with increased β-CD composition in the polymers and morphological changes were observed upon VE binding.
32553494	4	5	theme	inclusion	689:697	arg1	complexes					699:707	their VE inclusion complexes	680:707	their VE inclusion complexes	680:707	The host materials and their VE inclusion complexes are not cytotoxic, and are thus useful for VE and drug delivery.
32553494	0	6	theme	tocopherol	129:138	arg1	solubility					115:124	the water solubility	105:124	the water solubility of tocopherol	105:138	Hyaluronic-acid-based β-cyclodextrin grafted copolymers as biocompatible supramolecular hosts to enhance the water solubility of tocopherol.
32553494	3	7	theme	VE	508:509	arg1	capacity					519:526	VE loading capacity	508:526	VE loading capacity	508:526	VE loading capacity was directly correlated with increased β-CD composition in the polymers and morphological changes were observed upon VE binding.
32553494	1	8	dep	form	295:298	arg1	serving					377:383	serving	377:383	serving as a model drug delivery system	377:415	Hyaluronic acid (HA), a common biopolymer found in the extracellular fluid, was grafted with β-cyclodextrin (β-CD) to form a composite polymer that could form inclusion complexes with tocopherol (VE), enhancing its water-solubility and serving as a model drug delivery system.
32553494	1	8	dep	form	295:298	arg1	enhancing					342:350	enhancing	342:350	enhancing its water-solubility	342:371	Hyaluronic acid (HA), a common biopolymer found in the extracellular fluid, was grafted with β-cyclodextrin (β-CD) to form a composite polymer that could form inclusion complexes with tocopherol (VE), enhancing its water-solubility and serving as a model drug delivery system.
32553494	0	9	theme	Hyaluronic-acid-based	0:20	arg1	β-cyclodextrin					22:35	Hyaluronic-acid-based β-cyclodextrin	0:35	Hyaluronic-acid-based β-cyclodextrin	0:35	Hyaluronic-acid-based β-cyclodextrin grafted copolymers as biocompatible supramolecular hosts to enhance the water solubility of tocopherol.
32553494	0	10	theme	supramolecular	73:86	arg1	copolymers					45:54	copolymers	45:54	copolymers	45:54	Hyaluronic-acid-based β-cyclodextrin grafted copolymers as biocompatible supramolecular hosts to enhance the water solubility of tocopherol.
32553494	0	10	theme	supramolecular	73:86	arg1	hosts					88:92	biocompatible supramolecular hosts	59:92	biocompatible supramolecular hosts	59:92	Hyaluronic-acid-based β-cyclodextrin grafted copolymers as biocompatible supramolecular hosts to enhance the water solubility of tocopherol.
32553494	1	11	theme	composite	266:274	arg1	polymer					276:282	a composite polymer	264:282	a composite polymer that could form inclusion complexes with tocopherol (VE), enhancing its water-solubility and serving as a model drug delivery system	264:415	Hyaluronic acid (HA), a common biopolymer found in the extracellular fluid, was grafted with β-cyclodextrin (β-CD) to form a composite polymer that could form inclusion complexes with tocopherol (VE), enhancing its water-solubility and serving as a model drug delivery system.
32553494	1	12	theme	extracellular	196:208	arg1	fluid					210:214	the extracellular fluid	192:214	the extracellular fluid	192:214	Hyaluronic acid (HA), a common biopolymer found in the extracellular fluid, was grafted with β-cyclodextrin (β-CD) to form a composite polymer that could form inclusion complexes with tocopherol (VE), enhancing its water-solubility and serving as a model drug delivery system.
32553494	1	13	located	found	183:187	arg1	fluid					210:214	the extracellular fluid	192:214	the extracellular fluid	192:214	Hyaluronic acid (HA), a common biopolymer found in the extracellular fluid, was grafted with β-cyclodextrin (β-CD) to form a composite polymer that could form inclusion complexes with tocopherol (VE), enhancing its water-solubility and serving as a model drug delivery system.
32553494	1	13	located	found	183:187	arg2	acid					152:155	Hyaluronic acid	141:155	Hyaluronic acid (HA)	141:160	Hyaluronic acid (HA), a common biopolymer found in the extracellular fluid, was grafted with β-cyclodextrin (β-CD) to form a composite polymer that could form inclusion complexes with tocopherol (VE), enhancing its water-solubility and serving as a model drug delivery system.
32553494	1	13	located	found	183:187	arg2	biopolymer					172:181	a common biopolymer	163:181	a common biopolymer found in the extracellular fluid	163:214	Hyaluronic acid (HA), a common biopolymer found in the extracellular fluid, was grafted with β-cyclodextrin (β-CD) to form a composite polymer that could form inclusion complexes with tocopherol (VE), enhancing its water-solubility and serving as a model drug delivery system.
32553494	3	14	from	composition	572:582	arg1	polymers					591:598	the polymers	587:598	the polymers	587:598	VE loading capacity was directly correlated with increased β-CD composition in the polymers and morphological changes were observed upon VE binding.
32553494	0	15	theme	biocompatible	59:71	arg1	copolymers					45:54	copolymers	45:54	copolymers	45:54	Hyaluronic-acid-based β-cyclodextrin grafted copolymers as biocompatible supramolecular hosts to enhance the water solubility of tocopherol.
32553494	0	15	theme	biocompatible	59:71	arg1	hosts					88:92	biocompatible supramolecular hosts	59:92	biocompatible supramolecular hosts	59:92	Hyaluronic-acid-based β-cyclodextrin grafted copolymers as biocompatible supramolecular hosts to enhance the water solubility of tocopherol.
32553494	2	16	theme	β-CD	477:480	arg1	ratios					482:487	varying HA:β-CD ratios	466:487	varying HA:β-CD ratios	466:487	Herein, different copolymers were prepared with varying HA:β-CD ratios and characterized.
32553494	3	17	theme	increased	557:565	arg1	composition					572:582	increased β-CD composition	557:582	increased β-CD composition in the polymers	557:598	VE loading capacity was directly correlated with increased β-CD composition in the polymers and morphological changes were observed upon VE binding.
32553494	1	18	theme	Hyaluronic	141:150	arg1	biopolymer					172:181	a common biopolymer	163:181	a common biopolymer found in the extracellular fluid	163:214	Hyaluronic acid (HA), a common biopolymer found in the extracellular fluid, was grafted with β-cyclodextrin (β-CD) to form a composite polymer that could form inclusion complexes with tocopherol (VE), enhancing its water-solubility and serving as a model drug delivery system.
32553494	1	18	theme	Hyaluronic	141:150	arg1	HA					158:159	HA	158:159	HA	158:159	Hyaluronic acid (HA), a common biopolymer found in the extracellular fluid, was grafted with β-cyclodextrin (β-CD) to form a composite polymer that could form inclusion complexes with tocopherol (VE), enhancing its water-solubility and serving as a model drug delivery system.
32553494	1	18	theme	Hyaluronic	141:150	arg1	acid					152:155	Hyaluronic acid	141:155	Hyaluronic acid (HA)	141:160	Hyaluronic acid (HA), a common biopolymer found in the extracellular fluid, was grafted with β-cyclodextrin (β-CD) to form a composite polymer that could form inclusion complexes with tocopherol (VE), enhancing its water-solubility and serving as a model drug delivery system.
32553494	2	19	theme	different	426:434	arg1	copolymers					436:445	different copolymers	426:445	different copolymers	426:445	Herein, different copolymers were prepared with varying HA:β-CD ratios and characterized.
32553494	1	20	theme	inclusion	300:308	arg1	complexes					310:318	inclusion complexes	300:318	inclusion complexes	300:318	Hyaluronic acid (HA), a common biopolymer found in the extracellular fluid, was grafted with β-cyclodextrin (β-CD) to form a composite polymer that could form inclusion complexes with tocopherol (VE), enhancing its water-solubility and serving as a model drug delivery system.
32553494	3	21	theme	β-CD	567:570	arg1	composition					572:582	increased β-CD composition	557:582	increased β-CD composition in the polymers	557:598	VE loading capacity was directly correlated with increased β-CD composition in the polymers and morphological changes were observed upon VE binding.
32553494	4	22	theme	VE	686:687	arg1	complexes					699:707	their VE inclusion complexes	680:707	their VE inclusion complexes	680:707	The host materials and their VE inclusion complexes are not cytotoxic, and are thus useful for VE and drug delivery.
32553494	1	23	theme	model	390:394	arg1	system					410:415	a model drug delivery system	388:415	a model drug delivery system	388:415	Hyaluronic acid (HA), a common biopolymer found in the extracellular fluid, was grafted with β-cyclodextrin (β-CD) to form a composite polymer that could form inclusion complexes with tocopherol (VE), enhancing its water-solubility and serving as a model drug delivery system.
32553494	3	24	theme	VE	645:646	arg1	binding					648:654	VE binding	645:654	VE binding	645:654	VE loading capacity was directly correlated with increased β-CD composition in the polymers and morphological changes were observed upon VE binding.
32553494	3	25	theme	morphological	604:616	arg1	changes					618:624	morphological changes	604:624	morphological changes	604:624	VE loading capacity was directly correlated with increased β-CD composition in the polymers and morphological changes were observed upon VE binding.
32553494	1	26	theme	delivery	401:408	arg1	system					410:415	a model drug delivery system	388:415	a model drug delivery system	388:415	Hyaluronic acid (HA), a common biopolymer found in the extracellular fluid, was grafted with β-cyclodextrin (β-CD) to form a composite polymer that could form inclusion complexes with tocopherol (VE), enhancing its water-solubility and serving as a model drug delivery system.
32553494	2	27	theme	HA	474:475	arg1	ratios					482:487	varying HA:β-CD ratios	466:487	varying HA:β-CD ratios	466:487	Herein, different copolymers were prepared with varying HA:β-CD ratios and characterized.
32553494	4	28	theme	drug	759:762	arg1	delivery					764:771	drug delivery	759:771	drug delivery	759:771	The host materials and their VE inclusion complexes are not cytotoxic, and are thus useful for VE and drug delivery.
32553494	4	29	theme	host	661:664	arg1	useful					741:746	useful	741:746	useful	741:746	The host materials and their VE inclusion complexes are not cytotoxic, and are thus useful for VE and drug delivery.
32553494	4	29	theme	host	661:664	arg1	materials					666:674	The host materials	657:674	The host materials	657:674	The host materials and their VE inclusion complexes are not cytotoxic, and are thus useful for VE and drug delivery.
32553494	4	29	theme	host	661:664	arg1	cytotoxic					717:725	cytotoxic	717:725	cytotoxic	717:725	The host materials and their VE inclusion complexes are not cytotoxic, and are thus useful for VE and drug delivery.
34606788	0	0	theme	carboxymethyl	80:92	arg1	cellulose					94:102	carboxymethyl cellulose	80:102	carboxymethyl cellulose	80:102	Physical properties and antibacterial activity of the composited films based on carboxymethyl cellulose and gelatin functionalized with ε-polylysine.
34606788	3	1	from	spectrometer	748:759	arg1	calorimeter					835:845	differential scanning calorimeter	813:845	differential scanning calorimeter	813:845	The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter.
34606788	6	2	theme	present	1110:1116	arg1	results					1118:1124	The present results	1106:1124	The present results	1106:1124	The present results showed that the active films could be a potential material for food packaging.
34606788	3	3	from	peaks	708:712	arg1	Fourier					721:727	the Fourier	717:727	the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter	717:845	The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter.
34606788	2	4	theme	CMC-Gel	388:394	arg1	film					396:399	the control CMC-Gel film	376:399	the control CMC-Gel film	376:399	Compared with the control CMC-Gel film, the functionalized films had almost indistinguishable crystalline type, thickness, tensile strength, and elongation at break, however, poor water vapor barrier properties.
34606788	2	5	theme	barrier	554:560	arg1	properties					562:571	poor water vapor barrier properties	537:571	poor water vapor barrier properties	537:571	Compared with the control CMC-Gel film, the functionalized films had almost indistinguishable crystalline type, thickness, tensile strength, and elongation at break, however, poor water vapor barrier properties.
34606788	4	6	contain	containing	858:867	arg2	ε-PL					869:872	ε-PL	869:872	ε-PL	869:872	The films containing ε-PL showed excellent antibacterial activity against S. aureus, B. subtilis, E. coli and P. aeruginosa.
34606788	4	6	contain	containing	858:867	arg1	films					852:856	The films	848:856	The films containing ε-PL	848:872	The films containing ε-PL showed excellent antibacterial activity against S. aureus, B. subtilis, E. coli and P. aeruginosa.
34606788	2	7	theme	vapor	548:552	arg1	properties					562:571	poor water vapor barrier properties	537:571	poor water vapor barrier properties	537:571	Compared with the control CMC-Gel film, the functionalized films had almost indistinguishable crystalline type, thickness, tensile strength, and elongation at break, however, poor water vapor barrier properties.
34606788	6	8	theme	food	1189:1192	arg1	packaging					1194:1202	food packaging	1189:1202	food packaging	1189:1202	The present results showed that the active films could be a potential material for food packaging.
34606788	2	9	theme	water	542:546	arg1	vapor					548:552	poor water vapor	537:552	poor water vapor barrier properties	537:571	Compared with the control CMC-Gel film, the functionalized films had almost indistinguishable crystalline type, thickness, tensile strength, and elongation at break, however, poor water vapor barrier properties.
34606788	3	10	from	increase	769:776	arg1	calorimeter					835:845	differential scanning calorimeter	813:845	differential scanning calorimeter	813:845	The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter.
34606788	2	11	theme	poor	537:540	arg1	vapor					548:552	poor water vapor	537:552	poor water vapor barrier properties	537:571	Compared with the control CMC-Gel film, the functionalized films had almost indistinguishable crystalline type, thickness, tensile strength, and elongation at break, however, poor water vapor barrier properties.
34606788	4	12	theme	antibacterial	891:903	arg1	activity					905:912	excellent antibacterial activity	881:912	excellent antibacterial activity against S. aureus, B. subtilis, E. coli and P. aeruginosa	881:970	The films containing ε-PL showed excellent antibacterial activity against S. aureus, B. subtilis, E. coli and P. aeruginosa.
34606788	0	13	theme	Physical	0:7	arg1	properties					9:18	Physical properties	0:18	Physical properties	0:18	Physical properties and antibacterial activity of the composited films based on carboxymethyl cellulose and gelatin functionalized with ε-polylysine.
34606788	1	14	theme	carboxymethyl	199:211	arg1	blend					241:245	carboxymethyl cellulose-gelatin (CMC-Gel) blend	199:245	carboxymethyl cellulose-gelatin (CMC-Gel) blend with different concentration of ε-polylysine (ε-PL)	199:297	Antibacterial composite films were produced from carboxymethyl cellulose-gelatin (CMC-Gel) blend with different concentration of ε-polylysine (ε-PL) and their physical and chemical properties were characterized.
34606788	3	15	dep	transform	729:737	arg1	infrared					739:746	infrared	739:746	transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter	729:845	The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter.
34606788	5	16	theme	seventh	1036:1042	arg1	day					1044:1046	the seventh day	1032:1046	the seventh day	1032:1046	In the composting experiment, the films become degraded on the seventh day, and further degraded with the growth of molds over time.
34606788	6	17	theme	potential	1166:1174	arg1	material					1176:1183	a potential material	1164:1183	a potential material for food packaging	1164:1202	The present results showed that the active films could be a potential material for food packaging.
34606788	6	17	theme	potential	1166:1174	arg1	films					1149:1153	the active films	1138:1153	the active films	1138:1153	The present results showed that the active films could be a potential material for food packaging.
34606788	1	18	theme	cellulose-gelatin	213:229	arg1	blend					241:245	carboxymethyl cellulose-gelatin (CMC-Gel) blend	199:245	carboxymethyl cellulose-gelatin (CMC-Gel) blend with different concentration of ε-polylysine (ε-PL)	199:297	Antibacterial composite films were produced from carboxymethyl cellulose-gelatin (CMC-Gel) blend with different concentration of ε-polylysine (ε-PL) and their physical and chemical properties were characterized.
34606788	2	19	theme	tensile	485:491	arg1	strength					493:500	tensile strength	485:500	tensile strength	485:500	Compared with the control CMC-Gel film, the functionalized films had almost indistinguishable crystalline type, thickness, tensile strength, and elongation at break, however, poor water vapor barrier properties.
34606788	3	20	theme	glass	781:785	arg1	transition					787:796	glass transition	781:796	glass transition temperature	781:808	The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter.
34606788	5	21	theme	composting	980:989	arg1	experiment					991:1000	the composting experiment	976:1000	the composting experiment	976:1000	In the composting experiment, the films become degraded on the seventh day, and further degraded with the growth of molds over time.
34606788	2	22	theme	functionalized	406:419	arg1	films					421:425	the functionalized films	402:425	the functionalized films	402:425	Compared with the control CMC-Gel film, the functionalized films had almost indistinguishable crystalline type, thickness, tensile strength, and elongation at break, however, poor water vapor barrier properties.
34606788	0	23	theme	antibacterial	24:36	arg1	activity					38:45	antibacterial activity	24:45	antibacterial activity	24:45	Physical properties and antibacterial activity of the composited films based on carboxymethyl cellulose and gelatin functionalized with ε-polylysine.
34606788	1	24	theme	physical	309:316	arg1	properties					331:340	their physical and chemical properties	303:340	their physical and chemical properties	303:340	Antibacterial composite films were produced from carboxymethyl cellulose-gelatin (CMC-Gel) blend with different concentration of ε-polylysine (ε-PL) and their physical and chemical properties were characterized.
34606788	1	25	with	blend	241:245	arg1	concentration					262:274	different concentration	252:274	different concentration of ε-polylysine (ε-PL)	252:297	Antibacterial composite films were produced from carboxymethyl cellulose-gelatin (CMC-Gel) blend with different concentration of ε-polylysine (ε-PL) and their physical and chemical properties were characterized.
34606788	1	26	theme	CMC-Gel	232:238	arg1	blend					241:245	carboxymethyl cellulose-gelatin (CMC-Gel) blend	199:245	carboxymethyl cellulose-gelatin (CMC-Gel) blend with different concentration of ε-polylysine (ε-PL)	199:297	Antibacterial composite films were produced from carboxymethyl cellulose-gelatin (CMC-Gel) blend with different concentration of ε-polylysine (ε-PL) and their physical and chemical properties were characterized.
34606788	3	27	dep	Fourier	721:727	arg1	transform					729:737	transform	729:737	transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter	729:845	The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter.
34606788	5	28	theme	molds	1089:1093	arg1	growth					1079:1084	the growth	1075:1084	the growth of molds over time	1075:1103	In the composting experiment, the films become degraded on the seventh day, and further degraded with the growth of molds over time.
34606788	1	29	theme	chemical	322:329	arg1	properties					331:340	their physical and chemical properties	303:340	their physical and chemical properties	303:340	Antibacterial composite films were produced from carboxymethyl cellulose-gelatin (CMC-Gel) blend with different concentration of ε-polylysine (ε-PL) and their physical and chemical properties were characterized.
34606788	2	30	theme	crystalline	456:466	arg1	type					468:471	almost indistinguishable crystalline type	431:471	almost indistinguishable crystalline type	431:471	Compared with the control CMC-Gel film, the functionalized films had almost indistinguishable crystalline type, thickness, tensile strength, and elongation at break, however, poor water vapor barrier properties.
34606788	3	31	theme	differential	813:824	arg1	calorimeter					835:845	differential scanning calorimeter	813:845	differential scanning calorimeter	813:845	The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter.
34606788	2	32	contain	had	427:429	arg2	type					468:471	almost indistinguishable crystalline type	431:471	almost indistinguishable crystalline type	431:471	Compared with the control CMC-Gel film, the functionalized films had almost indistinguishable crystalline type, thickness, tensile strength, and elongation at break, however, poor water vapor barrier properties.
34606788	2	32	contain	had	427:429	arg2	properties					562:571	poor water vapor barrier properties	537:571	poor water vapor barrier properties	537:571	Compared with the control CMC-Gel film, the functionalized films had almost indistinguishable crystalline type, thickness, tensile strength, and elongation at break, however, poor water vapor barrier properties.
34606788	2	32	contain	had	427:429	arg2	elongation					507:516	elongation	507:516	elongation	507:516	Compared with the control CMC-Gel film, the functionalized films had almost indistinguishable crystalline type, thickness, tensile strength, and elongation at break, however, poor water vapor barrier properties.
34606788	2	32	contain	had	427:429	arg2	thickness					474:482	thickness	474:482	thickness	474:482	Compared with the control CMC-Gel film, the functionalized films had almost indistinguishable crystalline type, thickness, tensile strength, and elongation at break, however, poor water vapor barrier properties.
34606788	2	32	contain	had	427:429	arg2	strength					493:500	tensile strength	485:500	tensile strength	485:500	Compared with the control CMC-Gel film, the functionalized films had almost indistinguishable crystalline type, thickness, tensile strength, and elongation at break, however, poor water vapor barrier properties.
34606788	2	32	contain	had	427:429	arg1	films					421:425	the functionalized films	402:425	the functionalized films	402:425	Compared with the control CMC-Gel film, the functionalized films had almost indistinguishable crystalline type, thickness, tensile strength, and elongation at break, however, poor water vapor barrier properties.
34606788	2	33	theme	indistinguishable	438:454	arg1	type					468:471	almost indistinguishable crystalline type	431:471	almost indistinguishable crystalline type	431:471	Compared with the control CMC-Gel film, the functionalized films had almost indistinguishable crystalline type, thickness, tensile strength, and elongation at break, however, poor water vapor barrier properties.
34606788	0	34	theme	composited	54:63	arg1	films					65:69	the composited films	50:69	the composited films	50:69	Physical properties and antibacterial activity of the composited films based on carboxymethyl cellulose and gelatin functionalized with ε-polylysine.
34606788	3	35	theme	scanning	826:833	arg1	calorimeter					835:845	differential scanning calorimeter	813:845	differential scanning calorimeter	813:845	The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter.
34606788	3	36	theme	transition	787:796	arg1	temperature					798:808	glass transition temperature	781:808	glass transition temperature	781:808	The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter.
34606788	4	37	theme	excellent	881:889	arg1	activity					905:912	excellent antibacterial activity	881:912	excellent antibacterial activity against S. aureus, B. subtilis, E. coli and P. aeruginosa	881:970	The films containing ε-PL showed excellent antibacterial activity against S. aureus, B. subtilis, E. coli and P. aeruginosa.
34606788	2	38	theme	control	380:386	arg1	film					396:399	the control CMC-Gel film	376:399	the control CMC-Gel film	376:399	Compared with the control CMC-Gel film, the functionalized films had almost indistinguishable crystalline type, thickness, tensile strength, and elongation at break, however, poor water vapor barrier properties.
34606788	3	39	theme	temperature	798:808	arg1	spectrometer					748:759	spectrometer	748:759	spectrometer	748:759	The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter.
34606788	3	39	theme	temperature	798:808	arg1	increase					769:776	the increase	765:776	the increase of glass transition temperature in differential scanning calorimeter	765:845	The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter.
34606788	3	40	theme	absorption	697:706	arg1	peaks					708:712	absorption peaks	697:712	absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter	697:845	The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter.
34606788	6	41	theme	active	1142:1147	arg1	material					1176:1183	a potential material	1164:1183	a potential material for food packaging	1164:1202	The present results showed that the active films could be a potential material for food packaging.
34606788	6	41	theme	active	1142:1147	arg1	films					1149:1153	the active films	1138:1153	the active films	1138:1153	The present results showed that the active films could be a potential material for food packaging.
34606788	3	42	theme	peaks	708:712	arg1	changes					686:692	the changes	682:692	the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter	682:845	The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter.
34606788	3	42	theme	peaks	708:712	arg1	ε-PL					602:605	the ε-PL	598:605	the ε-PL	598:605	The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter.
34606788	3	43	from	Fourier	721:727	arg1	changes					686:692	the changes	682:692	the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter	682:845	The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter.
34606788	3	43	from	Fourier	721:727	arg1	ε-PL					602:605	the ε-PL	598:605	the ε-PL	598:605	The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter.
34606788	1	44	theme	different	252:260	arg1	concentration					262:274	different concentration	252:274	different concentration of ε-polylysine (ε-PL)	252:297	Antibacterial composite films were produced from carboxymethyl cellulose-gelatin (CMC-Gel) blend with different concentration of ε-polylysine (ε-PL) and their physical and chemical properties were characterized.
34606788	3	45	theme	electrostatic	652:664	arg1	interaction					666:676	electrostatic interaction	652:676	electrostatic interaction	652:676	The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter.
34606788	1	46	theme	Antibacterial	150:162	arg1	films					174:178	Antibacterial composite films	150:178	Antibacterial composite films	150:178	Antibacterial composite films were produced from carboxymethyl cellulose-gelatin (CMC-Gel) blend with different concentration of ε-polylysine (ε-PL) and their physical and chemical properties were characterized.
34606788	3	47	from	changes	686:692	arg1	Fourier					721:727	the Fourier	717:727	the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter	717:845	The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter.
34606788	3	48	theme	CMC-Gel	634:640	arg1	matrix					642:647	CMC-Gel matrix	634:647	CMC-Gel matrix	634:647	The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter.
34606788	1	49	theme	composite	164:172	arg1	films					174:178	Antibacterial composite films	150:178	Antibacterial composite films	150:178	Antibacterial composite films were produced from carboxymethyl cellulose-gelatin (CMC-Gel) blend with different concentration of ε-polylysine (ε-PL) and their physical and chemical properties were characterized.
34606788	0	50	theme	films	65:69	arg1	properties					9:18	Physical properties	0:18	Physical properties	0:18	Physical properties and antibacterial activity of the composited films based on carboxymethyl cellulose and gelatin functionalized with ε-polylysine.
34606788	0	50	theme	films	65:69	arg1	activity					38:45	antibacterial activity	24:45	antibacterial activity	24:45	Physical properties and antibacterial activity of the composited films based on carboxymethyl cellulose and gelatin functionalized with ε-polylysine.
34606788	1	51	theme	ε-polylysine	279:290	arg1	concentration					262:274	different concentration	252:274	different concentration of ε-polylysine (ε-PL)	252:297	Antibacterial composite films were produced from carboxymethyl cellulose-gelatin (CMC-Gel) blend with different concentration of ε-polylysine (ε-PL) and their physical and chemical properties were characterized.
34672616	4	0	theme	μm	603:604	arg1	scale					613:617	a μm length scale	601:617	a μm length scale in the presence of calcium chloride	601:653	Liesegang-like precipitation patterns develop on the outer surface on a μm length scale in the presence of calcium chloride, while the precipitate particles are distributed evenly in the bulk as corroborated by X-ray μ-CT.
34672616	4	1	theme	Liesegang-like	531:544	arg1	patterns					560:567	Liesegang-like precipitation patterns	531:567	Liesegang-like precipitation patterns	531:567	Liesegang-like precipitation patterns develop on the outer surface on a μm length scale in the presence of calcium chloride, while the precipitate particles are distributed evenly in the bulk as corroborated by X-ray μ-CT.
34672616	6	2	theme	pH	1063:1064	arg1	difference					1066:1075	the pH difference	1059:1075	the pH difference between the two sides of the membrane	1059:1113	Independent of the concentration of the weakly interacting salt, an electric potential gradient across the CS membrane develops, which vanishes when the pH difference between the two sides of the membrane diminishes.
34672616	4	3	from	scale	613:617	arg1	presence					626:633	the presence	622:633	the presence of calcium chloride	622:653	Liesegang-like precipitation patterns develop on the outer surface on a μm length scale in the presence of calcium chloride, while the precipitate particles are distributed evenly in the bulk as corroborated by X-ray μ-CT.
34672616	2	4	theme	inorganic	303:311	arg1	solution					336:343	inorganic salt and chitosan (CS) solution	303:343	inorganic salt and chitosan (CS) solution from below	303:354	Here, we report the instantaneous formation of soft tubular structures with a two-level hierarchy by injecting a mixture of inorganic salt and chitosan (CS) solution from below into a reactor filled with alkaline solution.
34672616	6	5	theme	membrane	1106:1113	arg1	sides					1093:1097	the two sides	1085:1097	the two sides of the membrane	1085:1113	Independent of the concentration of the weakly interacting salt, an electric potential gradient across the CS membrane develops, which vanishes when the pH difference between the two sides of the membrane diminishes.
34672616	6	6	theme	interacting	957:967	arg1	salt					969:972	the weakly interacting salt	946:972	the weakly interacting salt	946:972	Independent of the concentration of the weakly interacting salt, an electric potential gradient across the CS membrane develops, which vanishes when the pH difference between the two sides of the membrane diminishes.
34672616	4	7	theme	length	606:611	arg1	scale					613:617	a μm length scale	601:617	a μm length scale in the presence of calcium chloride	601:653	Liesegang-like precipitation patterns develop on the outer surface on a μm length scale in the presence of calcium chloride, while the precipitate particles are distributed evenly in the bulk as corroborated by X-ray μ-CT.
34672616	4	8	theme	precipitate	666:676	arg1	particles					678:686	the precipitate particles	662:686	the precipitate particles	662:686	Liesegang-like precipitation patterns develop on the outer surface on a μm length scale in the presence of calcium chloride, while the precipitate particles are distributed evenly in the bulk as corroborated by X-ray μ-CT.
34672616	6	9	theme	salt	969:972	arg1	concentration					929:941	the concentration	925:941	the concentration of the weakly interacting salt	925:972	Independent of the concentration of the weakly interacting salt, an electric potential gradient across the CS membrane develops, which vanishes when the pH difference between the two sides of the membrane diminishes.
34672616	5	10	theme	barium	773:778	arg1	hydroxide					780:788	barium hydroxide	773:788	barium hydroxide	773:788	On the other hand, barium hydroxide precipitates out only in the thin outer layer of the CS tubule when barium chloride is introduced into the CS solution.
34672616	4	11	from	surface	590:596	arg1	scale					613:617	a μm length scale	601:617	a μm length scale in the presence of calcium chloride	601:653	Liesegang-like precipitation patterns develop on the outer surface on a μm length scale in the presence of calcium chloride, while the precipitate particles are distributed evenly in the bulk as corroborated by X-ray μ-CT.
34672616	2	12	theme	alkaline	383:390	arg1	solution					392:399	alkaline solution	383:399	alkaline solution	383:399	Here, we report the instantaneous formation of soft tubular structures with a two-level hierarchy by injecting a mixture of inorganic salt and chitosan (CS) solution from below into a reactor filled with alkaline solution.
34672616	4	13	theme	outer	584:588	arg1	surface					590:596	the outer surface	580:596	the outer surface on a μm length scale in the presence of calcium chloride	580:653	Liesegang-like precipitation patterns develop on the outer surface on a μm length scale in the presence of calcium chloride, while the precipitate particles are distributed evenly in the bulk as corroborated by X-ray μ-CT.
34672616	0	14	theme	Hierarchical	0:11	arg1	Self-Assembly					13:25	Hierarchical Self-Assembly	0:25	Hierarchical Self-Assembly of Metal-Ion-Modulated Chitosan Tubules	0:65	Hierarchical Self-Assembly of Metal-Ion-Modulated Chitosan Tubules.
34672616	2	15	with	structures	239:248	arg1	hierarchy					267:275	a two-level hierarchy	255:275	a two-level hierarchy	255:275	Here, we report the instantaneous formation of soft tubular structures with a two-level hierarchy by injecting a mixture of inorganic salt and chitosan (CS) solution from below into a reactor filled with alkaline solution.
34672616	5	16	theme	CS	843:844	arg1	tubule					846:851	the CS tubule	839:851	the CS tubule	839:851	On the other hand, barium hydroxide precipitates out only in the thin outer layer of the CS tubule when barium chloride is introduced into the CS solution.
34672616	1	17	theme	biological	99:108	arg1	tissues					110:116	biological tissues	99:116	biological tissues	99:116	Soft materials such as gels or biological tissues can develop via self-assembly under chemo-mechanical forces.
34672616	6	18	theme	electric	978:985	arg1	gradient					997:1004	an electric potential gradient	975:1004	an electric potential gradient across the CS membrane	975:1027	Independent of the concentration of the weakly interacting salt, an electric potential gradient across the CS membrane develops, which vanishes when the pH difference between the two sides of the membrane diminishes.
34672616	5	19	theme	barium	858:863	arg1	chloride					865:872	barium chloride	858:872	barium chloride	858:872	On the other hand, barium hydroxide precipitates out only in the thin outer layer of the CS tubule when barium chloride is introduced into the CS solution.
34672616	0	20	theme	Metal-Ion-Modulated	30:48	arg1	Tubules					59:65	Metal-Ion-Modulated Chitosan Tubules	30:65	Metal-Ion-Modulated Chitosan Tubules	30:65	Hierarchical Self-Assembly of Metal-Ion-Modulated Chitosan Tubules.
34672616	4	21	theme	X-ray	742:746	arg1	μ-CT					748:751	X-ray μ-CT	742:751	X-ray μ-CT	742:751	Liesegang-like precipitation patterns develop on the outer surface on a μm length scale in the presence of calcium chloride, while the precipitate particles are distributed evenly in the bulk as corroborated by X-ray μ-CT.
34672616	4	22	theme	chloride	646:653	arg1	presence					626:633	the presence	622:633	the presence of calcium chloride	622:653	Liesegang-like precipitation patterns develop on the outer surface on a μm length scale in the presence of calcium chloride, while the precipitate particles are distributed evenly in the bulk as corroborated by X-ray μ-CT.
34672616	2	23	theme	chitosan	322:329	arg1	solution					336:343	inorganic salt and chitosan (CS) solution	303:343	inorganic salt and chitosan (CS) solution from below	303:354	Here, we report the instantaneous formation of soft tubular structures with a two-level hierarchy by injecting a mixture of inorganic salt and chitosan (CS) solution from below into a reactor filled with alkaline solution.
34672616	2	24	theme	salt	313:316	arg1	solution					336:343	inorganic salt and chitosan (CS) solution	303:343	inorganic salt and chitosan (CS) solution from below	303:354	Here, we report the instantaneous formation of soft tubular structures with a two-level hierarchy by injecting a mixture of inorganic salt and chitosan (CS) solution from below into a reactor filled with alkaline solution.
34672616	4	25	theme	calcium	638:644	arg1	chloride					646:653	calcium chloride	638:653	calcium chloride	638:653	Liesegang-like precipitation patterns develop on the outer surface on a μm length scale in the presence of calcium chloride, while the precipitate particles are distributed evenly in the bulk as corroborated by X-ray μ-CT.
34672616	0	26	theme	Tubules	59:65	arg1	Self-Assembly					13:25	Hierarchical Self-Assembly	0:25	Hierarchical Self-Assembly of Metal-Ion-Modulated Chitosan Tubules	0:65	Hierarchical Self-Assembly of Metal-Ion-Modulated Chitosan Tubules.
34672616	5	27	theme	CS	897:898	arg1	solution					900:907	the CS solution	893:907	the CS solution	893:907	On the other hand, barium hydroxide precipitates out only in the thin outer layer of the CS tubule when barium chloride is introduced into the CS solution.
34672616	0	28	theme	Chitosan	50:57	arg1	Tubules					59:65	Metal-Ion-Modulated Chitosan Tubules	30:65	Metal-Ion-Modulated Chitosan Tubules	30:65	Hierarchical Self-Assembly of Metal-Ion-Modulated Chitosan Tubules.
34672616	6	29	theme	concentration	929:941	arg1	Independent					910:920	Independent	910:920	Independent	910:920	Independent of the concentration of the weakly interacting salt, an electric potential gradient across the CS membrane develops, which vanishes when the pH difference between the two sides of the membrane diminishes.
34672616	3	30	theme	wrinkling	414:422	arg1	instabilities					424:436	Folding and wrinkling instabilities	402:436	Folding and wrinkling instabilities	402:436	Folding and wrinkling instabilities occur on the originally smooth surface controlled by the salt composition and concentration.
34672616	3	31	theme	smooth	462:467	arg1	surface					469:475	the originally smooth surface	447:475	the originally smooth surface controlled by the salt composition and concentration	447:528	Folding and wrinkling instabilities occur on the originally smooth surface controlled by the salt composition and concentration.
34672616	4	32	theme	precipitation	546:558	arg1	patterns					560:567	Liesegang-like precipitation patterns	531:567	Liesegang-like precipitation patterns	531:567	Liesegang-like precipitation patterns develop on the outer surface on a μm length scale in the presence of calcium chloride, while the precipitate particles are distributed evenly in the bulk as corroborated by X-ray μ-CT.
34672616	6	33	theme	CS	1017:1018	arg1	membrane					1020:1027	the CS membrane	1013:1027	the CS membrane	1013:1027	Independent of the concentration of the weakly interacting salt, an electric potential gradient across the CS membrane develops, which vanishes when the pH difference between the two sides of the membrane diminishes.
34672616	2	34	theme	structures	239:248	arg1	formation					213:221	the instantaneous formation	195:221	the instantaneous formation of soft tubular structures with a two-level hierarchy	195:275	Here, we report the instantaneous formation of soft tubular structures with a two-level hierarchy by injecting a mixture of inorganic salt and chitosan (CS) solution from below into a reactor filled with alkaline solution.
34672616	3	35	theme	salt	495:498	arg1	composition					500:510	the salt composition	491:510	the salt composition	491:510	Folding and wrinkling instabilities occur on the originally smooth surface controlled by the salt composition and concentration.
34672616	3	36	theme	Folding	402:408	arg1	instabilities					424:436	Folding and wrinkling instabilities	402:436	Folding and wrinkling instabilities	402:436	Folding and wrinkling instabilities occur on the originally smooth surface controlled by the salt composition and concentration.
34672616	2	37	theme	soft	226:229	arg1	structures					239:248	soft tubular structures	226:248	soft tubular structures with a two-level hierarchy	226:275	Here, we report the instantaneous formation of soft tubular structures with a two-level hierarchy by injecting a mixture of inorganic salt and chitosan (CS) solution from below into a reactor filled with alkaline solution.
34672616	2	38	theme	tubular	231:237	arg1	structures					239:248	soft tubular structures	226:248	soft tubular structures with a two-level hierarchy	226:275	Here, we report the instantaneous formation of soft tubular structures with a two-level hierarchy by injecting a mixture of inorganic salt and chitosan (CS) solution from below into a reactor filled with alkaline solution.
34672616	2	39	theme	solution	336:343	arg1	mixture					292:298	a mixture	290:298	a mixture of inorganic salt and chitosan (CS) solution from below	290:354	Here, we report the instantaneous formation of soft tubular structures with a two-level hierarchy by injecting a mixture of inorganic salt and chitosan (CS) solution from below into a reactor filled with alkaline solution.
34672616	1	40	theme	Soft	68:71	arg1	materials					73:81	Soft materials	68:81	Soft materials such as gels or biological tissues	68:116	Soft materials such as gels or biological tissues can develop via self-assembly under chemo-mechanical forces.
34672616	1	40	theme	Soft	68:71	arg1	gels					91:94	gels	91:94	gels	91:94	Soft materials such as gels or biological tissues can develop via self-assembly under chemo-mechanical forces.
34672616	1	40	theme	Soft	68:71	arg1	tissues					110:116	biological tissues	99:116	biological tissues	99:116	Soft materials such as gels or biological tissues can develop via self-assembly under chemo-mechanical forces.
34672616	5	41	theme	thin	819:822	arg1	layer					830:834	the thin outer layer	815:834	the thin outer layer of the CS tubule	815:851	On the other hand, barium hydroxide precipitates out only in the thin outer layer of the CS tubule when barium chloride is introduced into the CS solution.
34672616	2	42	theme	instantaneous	199:211	arg1	formation					213:221	the instantaneous formation	195:221	the instantaneous formation of soft tubular structures with a two-level hierarchy	195:275	Here, we report the instantaneous formation of soft tubular structures with a two-level hierarchy by injecting a mixture of inorganic salt and chitosan (CS) solution from below into a reactor filled with alkaline solution.
34672616	5	43	theme	outer	824:828	arg1	layer					830:834	the thin outer layer	815:834	the thin outer layer of the CS tubule	815:851	On the other hand, barium hydroxide precipitates out only in the thin outer layer of the CS tubule when barium chloride is introduced into the CS solution.
34672616	1	44	theme	chemo-mechanical	154:169	arg1	forces					171:176	chemo-mechanical forces	154:176	chemo-mechanical forces	154:176	Soft materials such as gels or biological tissues can develop via self-assembly under chemo-mechanical forces.
34672616	5	45	theme	tubule	846:851	arg1	layer					830:834	the thin outer layer	815:834	the thin outer layer of the CS tubule	815:851	On the other hand, barium hydroxide precipitates out only in the thin outer layer of the CS tubule when barium chloride is introduced into the CS solution.
34672616	5	46	theme	other	761:765	arg1	hand					767:770	the other hand	757:770	the other hand	757:770	On the other hand, barium hydroxide precipitates out only in the thin outer layer of the CS tubule when barium chloride is introduced into the CS solution.
34672616	2	47	theme	two-level	257:265	arg1	hierarchy					267:275	a two-level hierarchy	255:275	a two-level hierarchy	255:275	Here, we report the instantaneous formation of soft tubular structures with a two-level hierarchy by injecting a mixture of inorganic salt and chitosan (CS) solution from below into a reactor filled with alkaline solution.
34672616	6	48	theme	potential	987:995	arg1	gradient					997:1004	an electric potential gradient	975:1004	an electric potential gradient across the CS membrane	975:1027	Independent of the concentration of the weakly interacting salt, an electric potential gradient across the CS membrane develops, which vanishes when the pH difference between the two sides of the membrane diminishes.
34717974	0	0	theme	barley	74:79	arg1	performances					94:105	barley malt brewing performances	74:105	barley malt brewing performances	74:105	Molecular brewing: The molecular structural effects of starch adjuncts on barley malt brewing performances.
34717974	3	1	theme	maltotriose	790:800	arg1	content					742:748	the glucose content	730:748	the glucose content	730:748	High performance liquid chromatography results showed that depending on their molecular structures, starch adjuncts addition significantly increased wort maltose and maltotriose content, whereas reducing the glucose content and thus both the ratios of glucose and maltotriose to that of the maltose.
34717974	3	1	theme	maltotriose	790:800	arg1	ratios					768:773	thus both the ratios	754:773	thus both the ratios of glucose and maltotriose to that of the maltose	754:823	High performance liquid chromatography results showed that depending on their molecular structures, starch adjuncts addition significantly increased wort maltose and maltotriose content, whereas reducing the glucose content and thus both the ratios of glucose and maltotriose to that of the maltose.
34717974	4	2	theme	rest	937:940	arg1	72 h					942:945	the rest 72 h	933:945	the rest 72 h	933:945	The whole fermentation by dry beer yeast was finished within the first 48 h and reached to equilibrium for the rest 72 h, represented by the stable soluble protein content.
34717974	2	3	theme	beer	380:383	arg1	samples					385:391	freshly-fermented beer samples	362:391	freshly-fermented beer samples	362:391	Following fermentation, violate compounds of freshly-fermented beer samples were determined by headspace-solid-phase microextraction coupled with gas chromatography-mass spectrometry analysis (HS-SMPE-GC-MS).
34717974	6	4	from	content	1325:1331	arg1	contrary					1341:1348	the contrary	1337:1348	the contrary	1337:1348	The HS-SPME-GC-MS results showed that whether or not with starch adjuncts addition, the composition of violate compounds were not significantly influenced, their content, on the contrary, were altered, represented by different peak heights.
34717974	4	5	theme	soluble	974:980	arg1	content					990:996	the stable soluble protein content	963:996	the stable soluble protein content	963:996	The whole fermentation by dry beer yeast was finished within the first 48 h and reached to equilibrium for the rest 72 h, represented by the stable soluble protein content.
34717974	6	6	dep	altered	1356:1362	arg1	represented					1365:1375	represented	1365:1375	represented by different peak heights	1365:1401	The HS-SPME-GC-MS results showed that whether or not with starch adjuncts addition, the composition of violate compounds were not significantly influenced, their content, on the contrary, were altered, represented by different peak heights.
34717974	1	7	theme	starch	138:143	arg1	effects					127:133	the effects	123:133	the effects of starch adjuncts with different fine molecular structures obtained by size-exclusion chromatography on the mashing and fermentation efficiencies of barley malts	123:296	In this study, the effects of starch adjuncts with different fine molecular structures obtained by size-exclusion chromatography on the mashing and fermentation efficiencies of barley malts were investigated.
34717974	5	8	theme	starch	1040:1045	arg1	addition					1028:1035	the addition	1024:1035	the addition of starch	1024:1045	Results also showed that the addition of starch adjuncts resulted into increased alcohol content, which was mainly attributed to the altered glucose/maltose ratio.
34717974	0	9	theme	brewing	86:92	arg1	performances					94:105	barley malt brewing performances	74:105	barley malt brewing performances	74:105	Molecular brewing: The molecular structural effects of starch adjuncts on barley malt brewing performances.
34717974	3	10	theme	glucose	778:784	arg1	content					742:748	the glucose content	730:748	the glucose content	730:748	High performance liquid chromatography results showed that depending on their molecular structures, starch adjuncts addition significantly increased wort maltose and maltotriose content, whereas reducing the glucose content and thus both the ratios of glucose and maltotriose to that of the maltose.
34717974	3	10	theme	glucose	778:784	arg1	ratios					768:773	thus both the ratios	754:773	thus both the ratios of glucose and maltotriose to that of the maltose	754:823	High performance liquid chromatography results showed that depending on their molecular structures, starch adjuncts addition significantly increased wort maltose and maltotriose content, whereas reducing the glucose content and thus both the ratios of glucose and maltotriose to that of the maltose.
34717974	1	11	theme	fermentation	256:267	arg1	efficiencies					269:280	fermentation efficiencies	256:280	fermentation efficiencies	256:280	In this study, the effects of starch adjuncts with different fine molecular structures obtained by size-exclusion chromatography on the mashing and fermentation efficiencies of barley malts were investigated.
34717974	0	12	theme	malt	81:84	arg1	performances					94:105	barley malt brewing performances	74:105	barley malt brewing performances	74:105	Molecular brewing: The molecular structural effects of starch adjuncts on barley malt brewing performances.
34717974	1	13	with	effects	127:133	arg1	structures					184:193	different fine molecular structures	159:193	different fine molecular structures obtained by size-exclusion chromatography on the mashing and fermentation efficiencies of barley malts	159:296	In this study, the effects of starch adjuncts with different fine molecular structures obtained by size-exclusion chromatography on the mashing and fermentation efficiencies of barley malts were investigated.
34717974	7	14	theme	brewing	1501:1507	arg1	performances					1509:1520	brewing performances	1501:1520	brewing performances of barley malts	1501:1536	This study provides important information concerning the molecular effects of starch adjuncts on brewing performances of barley malts, and also provides a new pathway for choosing suitable types of adjuncts for making beer with better quality.
34717974	7	15	theme	better	1632:1637	arg1	quality					1639:1645	better quality	1632:1645	better quality	1632:1645	This study provides important information concerning the molecular effects of starch adjuncts on brewing performances of barley malts, and also provides a new pathway for choosing suitable types of adjuncts for making beer with better quality.
34717974	3	16	theme	performance	531:541	arg1	chromatography					550:563	High performance liquid chromatography	526:563	High performance liquid chromatography results	526:571	High performance liquid chromatography results showed that depending on their molecular structures, starch adjuncts addition significantly increased wort maltose and maltotriose content, whereas reducing the glucose content and thus both the ratios of glucose and maltotriose to that of the maltose.
34717974	3	17	theme	molecular	604:612	arg1	structures					614:623	their molecular structures	598:623	their molecular structures	598:623	High performance liquid chromatography results showed that depending on their molecular structures, starch adjuncts addition significantly increased wort maltose and maltotriose content, whereas reducing the glucose content and thus both the ratios of glucose and maltotriose to that of the maltose.
34717974	3	18	theme	High	526:529	arg1	chromatography					550:563	High performance liquid chromatography	526:563	High performance liquid chromatography results	526:571	High performance liquid chromatography results showed that depending on their molecular structures, starch adjuncts addition significantly increased wort maltose and maltotriose content, whereas reducing the glucose content and thus both the ratios of glucose and maltotriose to that of the maltose.
34717974	7	19	theme	barley	1525:1530	arg1	malts					1532:1536	barley malts	1525:1536	barley malts	1525:1536	This study provides important information concerning the molecular effects of starch adjuncts on brewing performances of barley malts, and also provides a new pathway for choosing suitable types of adjuncts for making beer with better quality.
34717974	4	20	theme	dry	852:854	arg1	yeast					861:865	dry beer yeast	852:865	dry beer yeast	852:865	The whole fermentation by dry beer yeast was finished within the first 48 h and reached to equilibrium for the rest 72 h, represented by the stable soluble protein content.
34717974	2	21	theme	headspace-solid-phase	412:432	arg1	microextraction					434:448	headspace-solid-phase microextraction	412:448	headspace-solid-phase microextraction coupled with gas chromatography-mass spectrometry analysis (HS-SMPE-GC-MS)	412:523	Following fermentation, violate compounds of freshly-fermented beer samples were determined by headspace-solid-phase microextraction coupled with gas chromatography-mass spectrometry analysis (HS-SMPE-GC-MS).
34717974	7	22	theme	malts	1532:1536	arg1	performances					1509:1520	brewing performances	1501:1520	brewing performances of barley malts	1501:1536	This study provides important information concerning the molecular effects of starch adjuncts on brewing performances of barley malts, and also provides a new pathway for choosing suitable types of adjuncts for making beer with better quality.
34717974	7	23	theme	starch	1482:1487	arg1	effects					1471:1477	the molecular effects	1457:1477	the molecular effects of starch adjuncts on brewing performances of barley malts	1457:1536	This study provides important information concerning the molecular effects of starch adjuncts on brewing performances of barley malts, and also provides a new pathway for choosing suitable types of adjuncts for making beer with better quality.
34717974	1	24	theme	different	159:167	arg1	structures					184:193	different fine molecular structures	159:193	different fine molecular structures obtained by size-exclusion chromatography on the mashing and fermentation efficiencies of barley malts	159:296	In this study, the effects of starch adjuncts with different fine molecular structures obtained by size-exclusion chromatography on the mashing and fermentation efficiencies of barley malts were investigated.
34717974	0	25	theme	Molecular	0:8	arg1	brewing					10:16	Molecular brewing	0:16	Molecular brewing: The molecular structural effects of starch	0:60	Molecular brewing: The molecular structural effects of starch adjuncts on barley malt brewing performances.
34717974	6	26	theme	violate	1266:1272	arg1	compounds					1274:1282	violate compounds	1266:1282	violate compounds	1266:1282	The HS-SPME-GC-MS results showed that whether or not with starch adjuncts addition, the composition of violate compounds were not significantly influenced, their content, on the contrary, were altered, represented by different peak heights.
34717974	7	27	theme	new	1559:1561	arg1	pathway					1563:1569	a new pathway	1557:1569	a new pathway for choosing suitable types of adjuncts for making beer with better quality	1557:1645	This study provides important information concerning the molecular effects of starch adjuncts on brewing performances of barley malts, and also provides a new pathway for choosing suitable types of adjuncts for making beer with better quality.
34717974	1	28	dep	starch	138:143	arg1	adjuncts					145:152	adjuncts	145:152	adjuncts	145:152	In this study, the effects of starch adjuncts with different fine molecular structures obtained by size-exclusion chromatography on the mashing and fermentation efficiencies of barley malts were investigated.
34717974	7	29	theme	important	1424:1432	arg1	information					1434:1444	important information	1424:1444	important information concerning the molecular effects of starch adjuncts on brewing performances of barley malts	1424:1536	This study provides important information concerning the molecular effects of starch adjuncts on brewing performances of barley malts, and also provides a new pathway for choosing suitable types of adjuncts for making beer with better quality.
34717974	1	30	theme	fine	169:172	arg1	structures					184:193	different fine molecular structures	159:193	different fine molecular structures obtained by size-exclusion chromatography on the mashing and fermentation efficiencies of barley malts	159:296	In this study, the effects of starch adjuncts with different fine molecular structures obtained by size-exclusion chromatography on the mashing and fermentation efficiencies of barley malts were investigated.
34717974	1	31	theme	barley	285:290	arg1	malts					292:296	barley malts	285:296	barley malts	285:296	In this study, the effects of starch adjuncts with different fine molecular structures obtained by size-exclusion chromatography on the mashing and fermentation efficiencies of barley malts were investigated.
34717974	4	32	theme	protein	982:988	arg1	content					990:996	the stable soluble protein content	963:996	the stable soluble protein content	963:996	The whole fermentation by dry beer yeast was finished within the first 48 h and reached to equilibrium for the rest 72 h, represented by the stable soluble protein content.
34717974	4	33	theme	whole	830:834	arg1	fermentation					836:847	The whole fermentation	826:847	The whole fermentation by dry beer yeast	826:865	The whole fermentation by dry beer yeast was finished within the first 48 h and reached to equilibrium for the rest 72 h, represented by the stable soluble protein content.
34717974	5	34	theme	alcohol	1080:1086	arg1	content					1088:1094	increased alcohol content	1070:1094	increased alcohol content	1070:1094	Results also showed that the addition of starch adjuncts resulted into increased alcohol content, which was mainly attributed to the altered glucose/maltose ratio.
34717974	2	35	theme	chromatography-mass	467:485	arg1	HS-SMPE-GC-MS					510:522	HS-SMPE-GC-MS	510:522	HS-SMPE-GC-MS	510:522	Following fermentation, violate compounds of freshly-fermented beer samples were determined by headspace-solid-phase microextraction coupled with gas chromatography-mass spectrometry analysis (HS-SMPE-GC-MS).
34717974	2	35	theme	chromatography-mass	467:485	arg1	analysis					500:507	gas chromatography-mass spectrometry analysis	463:507	gas chromatography-mass spectrometry analysis (HS-SMPE-GC-MS)	463:523	Following fermentation, violate compounds of freshly-fermented beer samples were determined by headspace-solid-phase microextraction coupled with gas chromatography-mass spectrometry analysis (HS-SMPE-GC-MS).
34717974	2	36	theme	spectrometry	487:498	arg1	HS-SMPE-GC-MS					510:522	HS-SMPE-GC-MS	510:522	HS-SMPE-GC-MS	510:522	Following fermentation, violate compounds of freshly-fermented beer samples were determined by headspace-solid-phase microextraction coupled with gas chromatography-mass spectrometry analysis (HS-SMPE-GC-MS).
34717974	2	36	theme	spectrometry	487:498	arg1	analysis					500:507	gas chromatography-mass spectrometry analysis	463:507	gas chromatography-mass spectrometry analysis (HS-SMPE-GC-MS)	463:523	Following fermentation, violate compounds of freshly-fermented beer samples were determined by headspace-solid-phase microextraction coupled with gas chromatography-mass spectrometry analysis (HS-SMPE-GC-MS).
34717974	1	37	theme	malts	292:296	arg1	mashing					244:250	mashing	244:250	mashing	244:250	In this study, the effects of starch adjuncts with different fine molecular structures obtained by size-exclusion chromatography on the mashing and fermentation efficiencies of barley malts were investigated.
34717974	1	37	theme	malts	292:296	arg1	efficiencies					269:280	fermentation efficiencies	256:280	fermentation efficiencies	256:280	In this study, the effects of starch adjuncts with different fine molecular structures obtained by size-exclusion chromatography on the mashing and fermentation efficiencies of barley malts were investigated.
34717974	4	38	theme	beer	856:859	arg1	yeast					861:865	dry beer yeast	852:865	dry beer yeast	852:865	The whole fermentation by dry beer yeast was finished within the first 48 h and reached to equilibrium for the rest 72 h, represented by the stable soluble protein content.
34717974	7	39	theme	adjuncts	1602:1609	arg1	types					1593:1597	suitable types	1584:1597	suitable types of adjuncts	1584:1609	This study provides important information concerning the molecular effects of starch adjuncts on brewing performances of barley malts, and also provides a new pathway for choosing suitable types of adjuncts for making beer with better quality.
34717974	2	40	theme	freshly-fermented	362:378	arg1	samples					385:391	freshly-fermented beer samples	362:391	freshly-fermented beer samples	362:391	Following fermentation, violate compounds of freshly-fermented beer samples were determined by headspace-solid-phase microextraction coupled with gas chromatography-mass spectrometry analysis (HS-SMPE-GC-MS).
34717974	2	41	theme	gas	463:465	arg1	HS-SMPE-GC-MS					510:522	HS-SMPE-GC-MS	510:522	HS-SMPE-GC-MS	510:522	Following fermentation, violate compounds of freshly-fermented beer samples were determined by headspace-solid-phase microextraction coupled with gas chromatography-mass spectrometry analysis (HS-SMPE-GC-MS).
34717974	2	41	theme	gas	463:465	arg1	analysis					500:507	gas chromatography-mass spectrometry analysis	463:507	gas chromatography-mass spectrometry analysis (HS-SMPE-GC-MS)	463:523	Following fermentation, violate compounds of freshly-fermented beer samples were determined by headspace-solid-phase microextraction coupled with gas chromatography-mass spectrometry analysis (HS-SMPE-GC-MS).
34717974	0	42	theme	structural	33:42	arg1	effects					44:50	The molecular structural effects	19:50	Molecular brewing: The molecular structural effects of starch	0:60	Molecular brewing: The molecular structural effects of starch adjuncts on barley malt brewing performances.
34717974	6	43	theme	peak	1390:1393	arg1	heights					1395:1401	different peak heights	1380:1401	different peak heights	1380:1401	The HS-SPME-GC-MS results showed that whether or not with starch adjuncts addition, the composition of violate compounds were not significantly influenced, their content, on the contrary, were altered, represented by different peak heights.
34717974	5	44	theme	altered	1132:1138	arg1	ratio					1156:1160	the altered glucose/maltose ratio	1128:1160	the altered glucose/maltose ratio	1128:1160	Results also showed that the addition of starch adjuncts resulted into increased alcohol content, which was mainly attributed to the altered glucose/maltose ratio.
34717974	4	45	theme	first	891:895	arg1	48 h					897:900	the first 48 h	887:900	the first 48 h	887:900	The whole fermentation by dry beer yeast was finished within the first 48 h and reached to equilibrium for the rest 72 h, represented by the stable soluble protein content.
34717974	6	46	dep	starch	1221:1226	arg1	adjuncts					1228:1235	adjuncts	1228:1235	adjuncts addition	1228:1244	The HS-SPME-GC-MS results showed that whether or not with starch adjuncts addition, the composition of violate compounds were not significantly influenced, their content, on the contrary, were altered, represented by different peak heights.
34717974	5	47	dep	adjuncts	1047:1054	arg1	resulted					1056:1063	resulted	1056:1063	adjuncts resulted into increased alcohol content, which was mainly attributed to the altered glucose/maltose ratio	1047:1160	Results also showed that the addition of starch adjuncts resulted into increased alcohol content, which was mainly attributed to the altered glucose/maltose ratio.
34717974	0	48	theme	molecular	23:31	arg1	effects					44:50	The molecular structural effects	19:50	Molecular brewing: The molecular structural effects of starch	0:60	Molecular brewing: The molecular structural effects of starch adjuncts on barley malt brewing performances.
34717974	6	49	theme	different	1380:1388	arg1	heights					1395:1401	different peak heights	1380:1401	different peak heights	1380:1401	The HS-SPME-GC-MS results showed that whether or not with starch adjuncts addition, the composition of violate compounds were not significantly influenced, their content, on the contrary, were altered, represented by different peak heights.
34717974	5	50	theme	glucose/maltose	1140:1154	arg1	ratio					1156:1160	the altered glucose/maltose ratio	1128:1160	the altered glucose/maltose ratio	1128:1160	Results also showed that the addition of starch adjuncts resulted into increased alcohol content, which was mainly attributed to the altered glucose/maltose ratio.
34717974	3	51	theme	maltotriose	692:702	arg1	content					704:710	maltotriose content	692:710	maltotriose content	692:710	High performance liquid chromatography results showed that depending on their molecular structures, starch adjuncts addition significantly increased wort maltose and maltotriose content, whereas reducing the glucose content and thus both the ratios of glucose and maltotriose to that of the maltose.
34717974	3	52	theme	glucose	734:740	arg1	content					742:748	the glucose content	730:748	the glucose content	730:748	High performance liquid chromatography results showed that depending on their molecular structures, starch adjuncts addition significantly increased wort maltose and maltotriose content, whereas reducing the glucose content and thus both the ratios of glucose and maltotriose to that of the maltose.
34717974	3	53	theme	wort	675:678	arg1	maltose					680:686	wort maltose	675:686	wort maltose	675:686	High performance liquid chromatography results showed that depending on their molecular structures, starch adjuncts addition significantly increased wort maltose and maltotriose content, whereas reducing the glucose content and thus both the ratios of glucose and maltotriose to that of the maltose.
34717974	7	54	dep	starch	1482:1487	arg1	adjuncts					1489:1496	adjuncts	1489:1496	adjuncts	1489:1496	This study provides important information concerning the molecular effects of starch adjuncts on brewing performances of barley malts, and also provides a new pathway for choosing suitable types of adjuncts for making beer with better quality.
34717974	7	55	from	effects	1471:1477	arg1	performances					1509:1520	brewing performances	1501:1520	brewing performances of barley malts	1501:1536	This study provides important information concerning the molecular effects of starch adjuncts on brewing performances of barley malts, and also provides a new pathway for choosing suitable types of adjuncts for making beer with better quality.
34717974	4	56	theme	stable	967:972	arg1	content					990:996	the stable soluble protein content	963:996	the stable soluble protein content	963:996	The whole fermentation by dry beer yeast was finished within the first 48 h and reached to equilibrium for the rest 72 h, represented by the stable soluble protein content.
34717974	7	57	theme	suitable	1584:1591	arg1	types					1593:1597	suitable types	1584:1597	suitable types of adjuncts	1584:1609	This study provides important information concerning the molecular effects of starch adjuncts on brewing performances of barley malts, and also provides a new pathway for choosing suitable types of adjuncts for making beer with better quality.
34717974	7	58	theme	molecular	1461:1469	arg1	effects					1471:1477	the molecular effects	1457:1477	the molecular effects of starch adjuncts on brewing performances of barley malts	1457:1536	This study provides important information concerning the molecular effects of starch adjuncts on brewing performances of barley malts, and also provides a new pathway for choosing suitable types of adjuncts for making beer with better quality.
34717974	2	59	theme	violate	341:347	arg1	compounds					349:357	violate compounds	341:357	violate compounds of freshly-fermented beer samples	341:391	Following fermentation, violate compounds of freshly-fermented beer samples were determined by headspace-solid-phase microextraction coupled with gas chromatography-mass spectrometry analysis (HS-SMPE-GC-MS).
34717974	1	60	theme	size-exclusion	207:220	arg1	chromatography					222:235	size-exclusion chromatography	207:235	size-exclusion chromatography on the mashing and fermentation efficiencies of barley malts	207:296	In this study, the effects of starch adjuncts with different fine molecular structures obtained by size-exclusion chromatography on the mashing and fermentation efficiencies of barley malts were investigated.
34717974	1	61	from	chromatography	222:235	arg1	mashing					244:250	mashing	244:250	mashing	244:250	In this study, the effects of starch adjuncts with different fine molecular structures obtained by size-exclusion chromatography on the mashing and fermentation efficiencies of barley malts were investigated.
34717974	1	61	from	chromatography	222:235	arg1	efficiencies					269:280	fermentation efficiencies	256:280	fermentation efficiencies	256:280	In this study, the effects of starch adjuncts with different fine molecular structures obtained by size-exclusion chromatography on the mashing and fermentation efficiencies of barley malts were investigated.
34717974	1	62	dep	mashing	244:250	arg1	the					240:242	the	240:242	the	240:242	In this study, the effects of starch adjuncts with different fine molecular structures obtained by size-exclusion chromatography on the mashing and fermentation efficiencies of barley malts were investigated.
34717974	6	63	theme	compounds	1274:1282	arg1	composition					1251:1261	the composition	1247:1261	the composition of violate compounds	1247:1282	The HS-SPME-GC-MS results showed that whether or not with starch adjuncts addition, the composition of violate compounds were not significantly influenced, their content, on the contrary, were altered, represented by different peak heights.
34717974	3	64	theme	chromatography	550:563	arg1	results					565:571	High performance liquid chromatography results	526:571	High performance liquid chromatography results	526:571	High performance liquid chromatography results showed that depending on their molecular structures, starch adjuncts addition significantly increased wort maltose and maltotriose content, whereas reducing the glucose content and thus both the ratios of glucose and maltotriose to that of the maltose.
34717974	3	65	theme	liquid	543:548	arg1	chromatography					550:563	High performance liquid chromatography	526:563	High performance liquid chromatography results	526:571	High performance liquid chromatography results showed that depending on their molecular structures, starch adjuncts addition significantly increased wort maltose and maltotriose content, whereas reducing the glucose content and thus both the ratios of glucose and maltotriose to that of the maltose.
34717974	6	66	theme	HS-SPME-GC-MS	1167:1179	arg1	results					1181:1187	The HS-SPME-GC-MS results	1163:1187	The HS-SPME-GC-MS results	1163:1187	The HS-SPME-GC-MS results showed that whether or not with starch adjuncts addition, the composition of violate compounds were not significantly influenced, their content, on the contrary, were altered, represented by different peak heights.
34717974	0	67	dep	brewing	10:16	arg1	effects					44:50	The molecular structural effects	19:50	Molecular brewing: The molecular structural effects of starch	0:60	Molecular brewing: The molecular structural effects of starch adjuncts on barley malt brewing performances.
34717974	1	68	theme	molecular	174:182	arg1	structures					184:193	different fine molecular structures	159:193	different fine molecular structures obtained by size-exclusion chromatography on the mashing and fermentation efficiencies of barley malts	159:296	In this study, the effects of starch adjuncts with different fine molecular structures obtained by size-exclusion chromatography on the mashing and fermentation efficiencies of barley malts were investigated.
34717974	0	69	theme	starch	55:60	arg1	effects					44:50	The molecular structural effects	19:50	Molecular brewing: The molecular structural effects of starch	0:60	Molecular brewing: The molecular structural effects of starch adjuncts on barley malt brewing performances.
34717974	5	70	theme	increased	1070:1078	arg1	content					1088:1094	increased alcohol content	1070:1094	increased alcohol content	1070:1094	Results also showed that the addition of starch adjuncts resulted into increased alcohol content, which was mainly attributed to the altered glucose/maltose ratio.
34717974	2	71	theme	samples	385:391	arg1	compounds					349:357	violate compounds	341:357	violate compounds of freshly-fermented beer samples	341:391	Following fermentation, violate compounds of freshly-fermented beer samples were determined by headspace-solid-phase microextraction coupled with gas chromatography-mass spectrometry analysis (HS-SMPE-GC-MS).
33712134	0	0	theme	composite	74:82	arg1	films					98:102	bacterial cellulose, curcumin, and chitosan composite biodegradable films	30:102	bacterial cellulose, curcumin, and chitosan composite biodegradable films for active packaging materials	30:133	Development and properties of bacterial cellulose, curcumin, and chitosan composite biodegradable films for active packaging materials.
33712134	1	1	theme	serious	149:155	arg1	damage					171:176	serious environmental damage	149:176	serious environmental damage resulting from plastic packaging materials	149:219	To deal with serious environmental damage resulting from plastic packaging materials, biodegradable films using natural products have gained considerable attention.
33712134	0	2	theme	chitosan	65:72	arg1	films					98:102	bacterial cellulose, curcumin, and chitosan composite biodegradable films	30:102	bacterial cellulose, curcumin, and chitosan composite biodegradable films for active packaging materials	30:133	Development and properties of bacterial cellulose, curcumin, and chitosan composite biodegradable films for active packaging materials.
33712134	1	3	theme	environmental	157:169	arg1	damage					171:176	serious environmental damage	149:176	serious environmental damage resulting from plastic packaging materials	149:219	To deal with serious environmental damage resulting from plastic packaging materials, biodegradable films using natural products have gained considerable attention.
33712134	0	4	theme	films	98:102	arg1	properties					16:25	properties	16:25	properties	16:25	Development and properties of bacterial cellulose, curcumin, and chitosan composite biodegradable films for active packaging materials.
33712134	0	4	theme	films	98:102	arg1	Development					0:10	Development	0:10	Development	0:10	Development and properties of bacterial cellulose, curcumin, and chitosan composite biodegradable films for active packaging materials.
33712134	3	5	theme	contact	622:628	arg1	angle					630:634	contact angle	622:634	contact angle (CA)	622:639	Composite films (CSn-BC-Cur) using CS with different molecular weights were investigated, and their water moisture content (MC), water solubility (WS), contact angle (CA), mechanical properties, barrier properties, and antioxidant properties were compared.
33712134	3	5	theme	contact	622:628	arg1	CA					637:638	CA	637:638	CA	637:638	Composite films (CSn-BC-Cur) using CS with different molecular weights were investigated, and their water moisture content (MC), water solubility (WS), contact angle (CA), mechanical properties, barrier properties, and antioxidant properties were compared.
33712134	1	6	with	deal	139:142	arg1	damage					171:176	serious environmental damage	149:176	serious environmental damage resulting from plastic packaging materials	149:219	To deal with serious environmental damage resulting from plastic packaging materials, biodegradable films using natural products have gained considerable attention.
33712134	5	7	theme	contact	868:874	arg1	angles					876:881	higher contact angles	861:881	higher contact angles	861:881	The results showed that chitosan with a higher molecular weight presented higher contact angles and mechanical properties, along with a lower moisture content, water vapor transmission rate, and oxygen transmission rate.
33712134	5	8	theme	higher	861:866	arg1	angles					876:881	higher contact angles	861:881	higher contact angles	861:881	The results showed that chitosan with a higher molecular weight presented higher contact angles and mechanical properties, along with a lower moisture content, water vapor transmission rate, and oxygen transmission rate.
33712134	1	9	theme	considerable	277:288	arg1	attention					290:298	considerable attention	277:298	considerable attention	277:298	To deal with serious environmental damage resulting from plastic packaging materials, biodegradable films using natural products have gained considerable attention.
33712134	0	10	theme	biodegradable	84:96	arg1	films					98:102	bacterial cellulose, curcumin, and chitosan composite biodegradable films	30:102	bacterial cellulose, curcumin, and chitosan composite biodegradable films for active packaging materials	30:133	Development and properties of bacterial cellulose, curcumin, and chitosan composite biodegradable films for active packaging materials.
33712134	5	11	theme	transmission	989:1000	arg1	rate					1002:1005	oxygen transmission rate	982:1005	oxygen transmission rate	982:1005	The results showed that chitosan with a higher molecular weight presented higher contact angles and mechanical properties, along with a lower moisture content, water vapor transmission rate, and oxygen transmission rate.
33712134	3	12	theme	Composite	470:478	arg1	films					480:484	Composite films	470:484	Composite films (CSn-BC-Cur) using CS with different molecular weights	470:539	Composite films (CSn-BC-Cur) using CS with different molecular weights were investigated, and their water moisture content (MC), water solubility (WS), contact angle (CA), mechanical properties, barrier properties, and antioxidant properties were compared.
33712134	3	12	theme	Composite	470:478	arg1	CSn-BC-Cur					487:496	CSn-BC-Cur	487:496	CSn-BC-Cur	487:496	Composite films (CSn-BC-Cur) using CS with different molecular weights were investigated, and their water moisture content (MC), water solubility (WS), contact angle (CA), mechanical properties, barrier properties, and antioxidant properties were compared.
33712134	5	13	theme	higher	827:832	arg1	weight					844:849	a higher molecular weight	825:849	a higher molecular weight	825:849	The results showed that chitosan with a higher molecular weight presented higher contact angles and mechanical properties, along with a lower moisture content, water vapor transmission rate, and oxygen transmission rate.
33712134	0	14	theme	active	108:113	arg1	materials					125:133	active packaging materials	108:133	active packaging materials	108:133	Development and properties of bacterial cellulose, curcumin, and chitosan composite biodegradable films for active packaging materials.
33712134	3	15	theme	mechanical	642:651	arg1	properties					653:662	mechanical properties	642:662	mechanical properties	642:662	Composite films (CSn-BC-Cur) using CS with different molecular weights were investigated, and their water moisture content (MC), water solubility (WS), contact angle (CA), mechanical properties, barrier properties, and antioxidant properties were compared.
33712134	6	16	theme	%	1062:1062	arg1	ethanol					1064:1070	95 % ethanol	1059:1070	95 % ethanol	1059:1070	Furthermore, when the composite film was placed in 95 % ethanol, it released active substances.
33712134	5	17	theme	mechanical	887:896	arg1	properties					898:907	mechanical properties	887:907	mechanical properties	887:907	The results showed that chitosan with a higher molecular weight presented higher contact angles and mechanical properties, along with a lower moisture content, water vapor transmission rate, and oxygen transmission rate.
33712134	5	18	with	chitosan	811:818	arg1	weight					844:849	a higher molecular weight	825:849	a higher molecular weight	825:849	The results showed that chitosan with a higher molecular weight presented higher contact angles and mechanical properties, along with a lower moisture content, water vapor transmission rate, and oxygen transmission rate.
33712134	5	19	theme	molecular	834:842	arg1	weight					844:849	a higher molecular weight	825:849	a higher molecular weight	825:849	The results showed that chitosan with a higher molecular weight presented higher contact angles and mechanical properties, along with a lower moisture content, water vapor transmission rate, and oxygen transmission rate.
33712134	3	20	theme	barrier	665:671	arg1	properties					673:682	barrier properties	665:682	barrier properties	665:682	Composite films (CSn-BC-Cur) using CS with different molecular weights were investigated, and their water moisture content (MC), water solubility (WS), contact angle (CA), mechanical properties, barrier properties, and antioxidant properties were compared.
33712134	2	21	theme	simple	320:325	arg1	route					363:367	a simple, fast, and environmentally-friendly route	318:367	a simple, fast, and environmentally-friendly route to construct a biodegradable film using chitosan (CS), bacterial cellulose (BC), and curcumin (Cur)	318:467	Here, we provide a simple, fast, and environmentally-friendly route to construct a biodegradable film using chitosan (CS), bacterial cellulose (BC), and curcumin (Cur).
33712134	2	22	theme	biodegradable	384:396	arg1	film					398:401	a biodegradable film	382:401	a biodegradable film using chitosan (CS), bacterial cellulose (BC), and curcumin (Cur)	382:467	Here, we provide a simple, fast, and environmentally-friendly route to construct a biodegradable film using chitosan (CS), bacterial cellulose (BC), and curcumin (Cur).
33712134	3	23	theme	different	513:521	arg1	weights					533:539	different molecular weights	513:539	different molecular weights	513:539	Composite films (CSn-BC-Cur) using CS with different molecular weights were investigated, and their water moisture content (MC), water solubility (WS), contact angle (CA), mechanical properties, barrier properties, and antioxidant properties were compared.
33712134	1	24	theme	packaging	201:209	arg1	materials					211:219	plastic packaging materials	193:219	plastic packaging materials	193:219	To deal with serious environmental damage resulting from plastic packaging materials, biodegradable films using natural products have gained considerable attention.
33712134	2	25	theme	bacterial	424:432	arg1	BC					445:446	BC	445:446	BC	445:446	Here, we provide a simple, fast, and environmentally-friendly route to construct a biodegradable film using chitosan (CS), bacterial cellulose (BC), and curcumin (Cur).
33712134	2	25	theme	bacterial	424:432	arg1	cellulose					434:442	bacterial cellulose	424:442	bacterial cellulose (BC)	424:447	Here, we provide a simple, fast, and environmentally-friendly route to construct a biodegradable film using chitosan (CS), bacterial cellulose (BC), and curcumin (Cur).
33712134	0	26	theme	packaging	115:123	arg1	materials					125:133	active packaging materials	108:133	active packaging materials	108:133	Development and properties of bacterial cellulose, curcumin, and chitosan composite biodegradable films for active packaging materials.
33712134	3	27	theme	molecular	523:531	arg1	weights					533:539	different molecular weights	513:539	different molecular weights	513:539	Composite films (CSn-BC-Cur) using CS with different molecular weights were investigated, and their water moisture content (MC), water solubility (WS), contact angle (CA), mechanical properties, barrier properties, and antioxidant properties were compared.
33712134	7	28	theme	food	1190:1193	arg1	packaging					1195:1203	food packaging	1190:1203	food packaging	1190:1203	The results suggest that these composite films can be used as promising materials for food packaging.
33712134	0	29	theme	cellulose	40:48	arg1	films					98:102	bacterial cellulose, curcumin, and chitosan composite biodegradable films	30:102	bacterial cellulose, curcumin, and chitosan composite biodegradable films for active packaging materials	30:133	Development and properties of bacterial cellulose, curcumin, and chitosan composite biodegradable films for active packaging materials.
33712134	3	30	theme	water	599:603	arg1	WS					617:618	WS	617:618	WS	617:618	Composite films (CSn-BC-Cur) using CS with different molecular weights were investigated, and their water moisture content (MC), water solubility (WS), contact angle (CA), mechanical properties, barrier properties, and antioxidant properties were compared.
33712134	3	30	theme	water	599:603	arg1	solubility					605:614	water solubility	599:614	water solubility (WS)	599:619	Composite films (CSn-BC-Cur) using CS with different molecular weights were investigated, and their water moisture content (MC), water solubility (WS), contact angle (CA), mechanical properties, barrier properties, and antioxidant properties were compared.
33712134	1	31	theme	plastic	193:199	arg1	materials					211:219	plastic packaging materials	193:219	plastic packaging materials	193:219	To deal with serious environmental damage resulting from plastic packaging materials, biodegradable films using natural products have gained considerable attention.
33712134	7	32	theme	promising	1166:1174	arg1	films					1145:1149	these composite films	1129:1149	these composite films	1129:1149	The results suggest that these composite films can be used as promising materials for food packaging.
33712134	7	32	theme	promising	1166:1174	arg1	materials					1176:1184	promising materials	1166:1184	promising materials for food packaging	1166:1203	The results suggest that these composite films can be used as promising materials for food packaging.
33712134	0	33	theme	bacterial	30:38	arg1	cellulose					40:48	bacterial cellulose	30:48	bacterial cellulose	30:48	Development and properties of bacterial cellulose, curcumin, and chitosan composite biodegradable films for active packaging materials.
33712134	3	34	theme	antioxidant	689:699	arg1	properties					701:710	antioxidant properties	689:710	antioxidant properties	689:710	Composite films (CSn-BC-Cur) using CS with different molecular weights were investigated, and their water moisture content (MC), water solubility (WS), contact angle (CA), mechanical properties, barrier properties, and antioxidant properties were compared.
33712134	4	35	theme	obtained	731:738	arg1	films					740:744	The obtained films	727:744	The obtained films	727:744	The obtained films were characterized by SEM, XRD, and TGA.
33712134	1	36	theme	biodegradable	222:234	arg1	films					236:240	biodegradable films	222:240	biodegradable films using natural products	222:263	To deal with serious environmental damage resulting from plastic packaging materials, biodegradable films using natural products have gained considerable attention.
33712134	0	37	theme	curcumin	51:58	arg1	films					98:102	bacterial cellulose, curcumin, and chitosan composite biodegradable films	30:102	bacterial cellulose, curcumin, and chitosan composite biodegradable films for active packaging materials	30:133	Development and properties of bacterial cellulose, curcumin, and chitosan composite biodegradable films for active packaging materials.
33712134	5	38	theme	oxygen	982:987	arg1	rate					1002:1005	oxygen transmission rate	982:1005	oxygen transmission rate	982:1005	The results showed that chitosan with a higher molecular weight presented higher contact angles and mechanical properties, along with a lower moisture content, water vapor transmission rate, and oxygen transmission rate.
33712134	5	39	theme	moisture	929:936	arg1	content					938:944	a lower moisture content	921:944	a lower moisture content	921:944	The results showed that chitosan with a higher molecular weight presented higher contact angles and mechanical properties, along with a lower moisture content, water vapor transmission rate, and oxygen transmission rate.
33712134	2	40	theme	environmentally-friendly	338:361	arg1	route					363:367	a simple, fast, and environmentally-friendly route	318:367	a simple, fast, and environmentally-friendly route to construct a biodegradable film using chitosan (CS), bacterial cellulose (BC), and curcumin (Cur)	318:467	Here, we provide a simple, fast, and environmentally-friendly route to construct a biodegradable film using chitosan (CS), bacterial cellulose (BC), and curcumin (Cur).
33712134	7	41	used	used	1158:1161	arg2	films					1145:1149	these composite films	1129:1149	these composite films	1129:1149	The results suggest that these composite films can be used as promising materials for food packaging.
33712134	7	41	used	used	1158:1161	arg2	materials					1176:1184	promising materials	1166:1184	promising materials for food packaging	1166:1203	The results suggest that these composite films can be used as promising materials for food packaging.
33712134	5	42	theme	water	947:951	arg1	rate					972:975	water vapor transmission rate	947:975	water vapor transmission rate	947:975	The results showed that chitosan with a higher molecular weight presented higher contact angles and mechanical properties, along with a lower moisture content, water vapor transmission rate, and oxygen transmission rate.
33712134	5	43	theme	lower	923:927	arg1	content					938:944	a lower moisture content	921:944	a lower moisture content	921:944	The results showed that chitosan with a higher molecular weight presented higher contact angles and mechanical properties, along with a lower moisture content, water vapor transmission rate, and oxygen transmission rate.
33712134	6	44	theme	95	1059:1060	arg1	%					1062:1062	%	1062:1062	%	1062:1062	Furthermore, when the composite film was placed in 95 % ethanol, it released active substances.
33712134	5	45	theme	vapor	953:957	arg1	rate					972:975	water vapor transmission rate	947:975	water vapor transmission rate	947:975	The results showed that chitosan with a higher molecular weight presented higher contact angles and mechanical properties, along with a lower moisture content, water vapor transmission rate, and oxygen transmission rate.
33712134	6	46	theme	active	1085:1090	arg1	substances					1092:1101	active substances	1085:1101	active substances	1085:1101	Furthermore, when the composite film was placed in 95 % ethanol, it released active substances.
33712134	2	47	theme	fast	328:331	arg1	route					363:367	a simple, fast, and environmentally-friendly route	318:367	a simple, fast, and environmentally-friendly route to construct a biodegradable film using chitosan (CS), bacterial cellulose (BC), and curcumin (Cur)	318:467	Here, we provide a simple, fast, and environmentally-friendly route to construct a biodegradable film using chitosan (CS), bacterial cellulose (BC), and curcumin (Cur).
33712134	6	48	theme	composite	1030:1038	arg1	film					1040:1043	the composite film	1026:1043	the composite film	1026:1043	Furthermore, when the composite film was placed in 95 % ethanol, it released active substances.
33712134	5	49	theme	transmission	959:970	arg1	rate					972:975	water vapor transmission rate	947:975	water vapor transmission rate	947:975	The results showed that chitosan with a higher molecular weight presented higher contact angles and mechanical properties, along with a lower moisture content, water vapor transmission rate, and oxygen transmission rate.
33712134	3	50	theme	water	570:574	arg1	MC					594:595	MC	594:595	MC	594:595	Composite films (CSn-BC-Cur) using CS with different molecular weights were investigated, and their water moisture content (MC), water solubility (WS), contact angle (CA), mechanical properties, barrier properties, and antioxidant properties were compared.
33712134	3	50	theme	water	570:574	arg1	content					585:591	their water moisture content	564:591	their water moisture content (MC)	564:596	Composite films (CSn-BC-Cur) using CS with different molecular weights were investigated, and their water moisture content (MC), water solubility (WS), contact angle (CA), mechanical properties, barrier properties, and antioxidant properties were compared.
33712134	7	51	theme	composite	1135:1143	arg1	films					1145:1149	these composite films	1129:1149	these composite films	1129:1149	The results suggest that these composite films can be used as promising materials for food packaging.
33712134	7	51	theme	composite	1135:1143	arg1	materials					1176:1184	promising materials	1166:1184	promising materials for food packaging	1166:1203	The results suggest that these composite films can be used as promising materials for food packaging.
33712134	1	52	theme	natural	248:254	arg1	products					256:263	natural products	248:263	natural products	248:263	To deal with serious environmental damage resulting from plastic packaging materials, biodegradable films using natural products have gained considerable attention.
33712134	3	53	theme	moisture	576:583	arg1	MC					594:595	MC	594:595	MC	594:595	Composite films (CSn-BC-Cur) using CS with different molecular weights were investigated, and their water moisture content (MC), water solubility (WS), contact angle (CA), mechanical properties, barrier properties, and antioxidant properties were compared.
33712134	3	53	theme	moisture	576:583	arg1	content					585:591	their water moisture content	564:591	their water moisture content (MC)	564:596	Composite films (CSn-BC-Cur) using CS with different molecular weights were investigated, and their water moisture content (MC), water solubility (WS), contact angle (CA), mechanical properties, barrier properties, and antioxidant properties were compared.
35027125	0	0	theme	graphitic	92:100	arg1	nitride/cellulose					109:125	aqueous stable graphitic carbon nitride/cellulose	77:125	aqueous stable graphitic carbon nitride/cellulose	77:125	Increased ion transport and high-efficient osmotic energy conversion through aqueous stable graphitic carbon nitride/cellulose nanofiber composite membrane.
35027125	7	1	theme	nanofluidic	1227:1237	arg1	devices					1239:1245	cellulose-based nanofluidic devices	1211:1245	cellulose-based nanofluidic devices	1211:1245	This work could inspire new designs of cellulose-based nanofluidic devices for improved osmotic energy conversion.
35027125	3	2	theme	charge-governed	551:565	arg1	conductivity					571:582	charge-governed ion conductivity	551:582	charge-governed ion conductivity	551:582	Composite membranes exhibiting charge-governed ion conductivity were prepared via co-homogenization of g-C3N4 with CNF and vacuum filtration.
35027125	6	3	theme	nanofluidic	1080:1090	arg1	conversion					1107:1116	nanofluidic osmotic energy conversion	1080:1116	nanofluidic osmotic energy conversion	1080:1116	We demonstrate the potential of the composite membranes in nanofluidic osmotic energy conversion, displaying thermo-enhanced power output performance.
35027125	0	4	theme	stable	85:90	arg1	nitride/cellulose					109:125	aqueous stable graphitic carbon nitride/cellulose	77:125	aqueous stable graphitic carbon nitride/cellulose	77:125	Increased ion transport and high-efficient osmotic energy conversion through aqueous stable graphitic carbon nitride/cellulose nanofiber composite membrane.
35027125	5	5	from	concentrations	860:873	arg1	conductivity					824:835	Higher ion conductivity	813:835	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl)	813:887	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl) was achieved due to the increased charge density of the lamellar nanochannels and the excellent aqueous stability of the membranes.
35027125	7	6	theme	devices	1239:1245	arg1	designs					1200:1206	new designs	1196:1206	new designs of cellulose-based nanofluidic devices for improved osmotic energy conversion	1196:1284	This work could inspire new designs of cellulose-based nanofluidic devices for improved osmotic energy conversion.
35027125	2	7	theme	g-C3N4	376:381	arg1	membranes					420:428	graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes	350:428	graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels	350:464	Herein, we fabricate graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels for capturing osmotic energy from salinity gradients.
35027125	4	8	from	content	770:776	arg1	membranes					802:810	the composite membranes	788:810	the composite membranes	788:810	Ion conductivity was efficiently modulated by fine-tuning the charge density through controlling the weight content of CNF in the composite membranes.
35027125	5	9	theme	<10-2 M	876:882	arg1	concentrations					860:873	low concentrations	856:873	low concentrations (<10-2 M KCl)	856:887	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl) was achieved due to the increased charge density of the lamellar nanochannels and the excellent aqueous stability of the membranes.
35027125	5	9	theme	<10-2 M	876:882	arg1	KCl					884:886	<10-2 M KCl	876:886	<10-2 M KCl	876:886	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl) was achieved due to the increased charge density of the lamellar nanochannels and the excellent aqueous stability of the membranes.
35027125	6	10	theme	thermo-enhanced	1130:1144	arg1	output					1152:1157	thermo-enhanced power output	1130:1157	thermo-enhanced power output performance	1130:1169	We demonstrate the potential of the composite membranes in nanofluidic osmotic energy conversion, displaying thermo-enhanced power output performance.
35027125	1	11	theme	energy	286:291	arg1	crisis					293:298	the energy crisis	282:298	the energy crisis	282:298	Increased attention has evoked on the utilization of renewable energy, particularly osmotic power as a potential solution to the energy crisis and environmental pollution.
35027125	0	12	theme	carbon	102:107	arg1	nitride/cellulose					109:125	aqueous stable graphitic carbon nitride/cellulose	77:125	aqueous stable graphitic carbon nitride/cellulose	77:125	Increased ion transport and high-efficient osmotic energy conversion through aqueous stable graphitic carbon nitride/cellulose nanofiber composite membrane.
35027125	7	13	theme	cellulose-based	1211:1225	arg1	devices					1239:1245	cellulose-based nanofluidic devices	1211:1245	cellulose-based nanofluidic devices	1211:1245	This work could inspire new designs of cellulose-based nanofluidic devices for improved osmotic energy conversion.
35027125	4	14	theme	CNF	781:783	arg1	content					770:776	the weight content	759:776	the weight content of CNF in the composite membranes	759:810	Ion conductivity was efficiently modulated by fine-tuning the charge density through controlling the weight content of CNF in the composite membranes.
35027125	3	15	theme	Composite	520:528	arg1	membranes					530:538	Composite membranes	520:538	Composite membranes exhibiting charge-governed ion conductivity	520:582	Composite membranes exhibiting charge-governed ion conductivity were prepared via co-homogenization of g-C3N4 with CNF and vacuum filtration.
35027125	0	16	theme	composite	137:145	arg1	membrane					147:154	composite membrane	137:154	composite membrane	137:154	Increased ion transport and high-efficient osmotic energy conversion through aqueous stable graphitic carbon nitride/cellulose nanofiber composite membrane.
35027125	2	17	with	membranes	420:428	arg1	nanochannels					453:464	tailored lamellar nanochannels	435:464	tailored lamellar nanochannels	435:464	Herein, we fabricate graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels for capturing osmotic energy from salinity gradients.
35027125	7	18	theme	new	1196:1198	arg1	designs					1200:1206	new designs	1196:1206	new designs of cellulose-based nanofluidic devices for improved osmotic energy conversion	1196:1284	This work could inspire new designs of cellulose-based nanofluidic devices for improved osmotic energy conversion.
35027125	6	19	theme	power	1146:1150	arg1	output					1152:1157	thermo-enhanced power output	1130:1157	thermo-enhanced power output performance	1130:1169	We demonstrate the potential of the composite membranes in nanofluidic osmotic energy conversion, displaying thermo-enhanced power output performance.
35027125	2	20	theme	CNF	405:407	arg1	membranes					420:428	graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes	350:428	graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels	350:464	Herein, we fabricate graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels for capturing osmotic energy from salinity gradients.
35027125	2	21	theme	composite	410:418	arg1	membranes					420:428	graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes	350:428	graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels	350:464	Herein, we fabricate graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels for capturing osmotic energy from salinity gradients.
35027125	6	22	theme	composite	1057:1065	arg1	membranes					1067:1075	the composite membranes	1053:1075	the composite membranes	1053:1075	We demonstrate the potential of the composite membranes in nanofluidic osmotic energy conversion, displaying thermo-enhanced power output performance.
35027125	2	23	from	gradients	509:517	arg1	energy					488:493	osmotic energy	480:493	osmotic energy from salinity gradients	480:517	Herein, we fabricate graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels for capturing osmotic energy from salinity gradients.
35027125	7	24	theme	osmotic	1260:1266	arg1	conversion					1275:1284	improved osmotic energy conversion	1251:1284	improved osmotic energy conversion	1251:1284	This work could inspire new designs of cellulose-based nanofluidic devices for improved osmotic energy conversion.
35027125	2	25	theme	osmotic	480:486	arg1	energy					488:493	osmotic energy	480:493	osmotic energy from salinity gradients	480:517	Herein, we fabricate graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels for capturing osmotic energy from salinity gradients.
35027125	5	26	theme	ion	820:822	arg1	conductivity					824:835	Higher ion conductivity	813:835	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl)	813:887	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl) was achieved due to the increased charge density of the lamellar nanochannels and the excellent aqueous stability of the membranes.
35027125	0	27	theme	ion	10:12	arg1	transport					14:22	Increased ion transport	0:22	Increased ion transport	0:22	Increased ion transport and high-efficient osmotic energy conversion through aqueous stable graphitic carbon nitride/cellulose nanofiber composite membrane.
35027125	5	28	theme	excellent	975:983	arg1	stability					993:1001	the excellent aqueous stability	971:1001	the excellent aqueous stability of the membranes	971:1018	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl) was achieved due to the increased charge density of the lamellar nanochannels and the excellent aqueous stability of the membranes.
35027125	5	29	theme	membranes	1010:1018	arg1	stability					993:1001	the excellent aqueous stability	971:1001	the excellent aqueous stability of the membranes	971:1018	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl) was achieved due to the increased charge density of the lamellar nanochannels and the excellent aqueous stability of the membranes.
35027125	5	29	theme	membranes	1010:1018	arg1	density					930:936	the increased charge density	909:936	the increased charge density of the lamellar nanochannels	909:965	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl) was achieved due to the increased charge density of the lamellar nanochannels and the excellent aqueous stability of the membranes.
35027125	1	30	theme	renewable	210:218	arg1	energy					220:225	renewable energy	210:225	renewable energy	210:225	Increased attention has evoked on the utilization of renewable energy, particularly osmotic power as a potential solution to the energy crisis and environmental pollution.
35027125	1	30	theme	renewable	210:218	arg1	power					249:253	osmotic power	241:253	particularly osmotic power	228:253	Increased attention has evoked on the utilization of renewable energy, particularly osmotic power as a potential solution to the energy crisis and environmental pollution.
35027125	0	31	theme	Increased	0:8	arg1	transport					14:22	Increased ion transport	0:22	Increased ion transport	0:22	Increased ion transport and high-efficient osmotic energy conversion through aqueous stable graphitic carbon nitride/cellulose nanofiber composite membrane.
35027125	7	32	theme	energy	1268:1273	arg1	conversion					1275:1284	improved osmotic energy conversion	1251:1284	improved osmotic energy conversion	1251:1284	This work could inspire new designs of cellulose-based nanofluidic devices for improved osmotic energy conversion.
35027125	4	33	theme	composite	792:800	arg1	membranes					802:810	the composite membranes	788:810	the composite membranes	788:810	Ion conductivity was efficiently modulated by fine-tuning the charge density through controlling the weight content of CNF in the composite membranes.
35027125	1	34	theme	energy	220:225	arg1	utilization					195:205	the utilization	191:205	the utilization of renewable energy, particularly osmotic power as a potential solution to the energy crisis and environmental pollution	191:326	Increased attention has evoked on the utilization of renewable energy, particularly osmotic power as a potential solution to the energy crisis and environmental pollution.
35027125	5	35	theme	increased	913:921	arg1	density					930:936	the increased charge density	909:936	the increased charge density of the lamellar nanochannels	909:965	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl) was achieved due to the increased charge density of the lamellar nanochannels and the excellent aqueous stability of the membranes.
35027125	1	36	theme	environmental	304:316	arg1	pollution					318:326	environmental pollution	304:326	environmental pollution	304:326	Increased attention has evoked on the utilization of renewable energy, particularly osmotic power as a potential solution to the energy crisis and environmental pollution.
35027125	6	37	from	potential	1040:1048	arg1	conversion					1107:1116	nanofluidic osmotic energy conversion	1080:1116	nanofluidic osmotic energy conversion	1080:1116	We demonstrate the potential of the composite membranes in nanofluidic osmotic energy conversion, displaying thermo-enhanced power output performance.
35027125	3	38	theme	g-C3N4	623:628	arg1	co-homogenization					602:618	co-homogenization	602:618	co-homogenization of g-C3N4 with CNF and vacuum filtration	602:659	Composite membranes exhibiting charge-governed ion conductivity were prepared via co-homogenization of g-C3N4 with CNF and vacuum filtration.
35027125	2	39	theme	lamellar	444:451	arg1	nanochannels					453:464	tailored lamellar nanochannels	435:464	tailored lamellar nanochannels	435:464	Herein, we fabricate graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels for capturing osmotic energy from salinity gradients.
35027125	3	40	theme	ion	567:569	arg1	conductivity					571:582	charge-governed ion conductivity	551:582	charge-governed ion conductivity	551:582	Composite membranes exhibiting charge-governed ion conductivity were prepared via co-homogenization of g-C3N4 with CNF and vacuum filtration.
35027125	2	41	theme	tailored	435:442	arg1	nanochannels					453:464	tailored lamellar nanochannels	435:464	tailored lamellar nanochannels	435:464	Herein, we fabricate graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels for capturing osmotic energy from salinity gradients.
35027125	0	42	theme	osmotic	43:49	arg1	conversion					58:67	high-efficient osmotic energy conversion	28:67	high-efficient osmotic energy conversion	28:67	Increased ion transport and high-efficient osmotic energy conversion through aqueous stable graphitic carbon nitride/cellulose nanofiber composite membrane.
35027125	4	43	theme	charge	724:729	arg1	density					731:737	the charge density	720:737	the charge density	720:737	Ion conductivity was efficiently modulated by fine-tuning the charge density through controlling the weight content of CNF in the composite membranes.
35027125	5	44	theme	lamellar	945:952	arg1	nanochannels					954:965	the lamellar nanochannels	941:965	the lamellar nanochannels	941:965	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl) was achieved due to the increased charge density of the lamellar nanochannels and the excellent aqueous stability of the membranes.
35027125	1	45	theme	osmotic	241:247	arg1	energy					220:225	renewable energy	210:225	renewable energy	210:225	Increased attention has evoked on the utilization of renewable energy, particularly osmotic power as a potential solution to the energy crisis and environmental pollution.
35027125	1	45	theme	osmotic	241:247	arg1	power					249:253	osmotic power	241:253	particularly osmotic power	228:253	Increased attention has evoked on the utilization of renewable energy, particularly osmotic power as a potential solution to the energy crisis and environmental pollution.
35027125	0	46	theme	high-efficient	28:41	arg1	conversion					58:67	high-efficient osmotic energy conversion	28:67	high-efficient osmotic energy conversion	28:67	Increased ion transport and high-efficient osmotic energy conversion through aqueous stable graphitic carbon nitride/cellulose nanofiber composite membrane.
35027125	2	47	theme	salinity	500:507	arg1	gradients					509:517	salinity gradients	500:517	salinity gradients	500:517	Herein, we fabricate graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels for capturing osmotic energy from salinity gradients.
35027125	6	48	theme	energy	1100:1105	arg1	conversion					1107:1116	nanofluidic osmotic energy conversion	1080:1116	nanofluidic osmotic energy conversion	1080:1116	We demonstrate the potential of the composite membranes in nanofluidic osmotic energy conversion, displaying thermo-enhanced power output performance.
35027125	5	49	theme	charge	923:928	arg1	density					930:936	the increased charge density	909:936	the increased charge density of the lamellar nanochannels	909:965	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl) was achieved due to the increased charge density of the lamellar nanochannels and the excellent aqueous stability of the membranes.
35027125	3	50	with	co-homogenization	602:618	arg1	CNF					635:637	CNF	635:637	CNF	635:637	Composite membranes exhibiting charge-governed ion conductivity were prepared via co-homogenization of g-C3N4 with CNF and vacuum filtration.
35027125	3	50	with	co-homogenization	602:618	arg1	filtration					650:659	vacuum filtration	643:659	vacuum filtration	643:659	Composite membranes exhibiting charge-governed ion conductivity were prepared via co-homogenization of g-C3N4 with CNF and vacuum filtration.
35027125	5	51	theme	nanochannels	954:965	arg1	stability					993:1001	the excellent aqueous stability	971:1001	the excellent aqueous stability of the membranes	971:1018	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl) was achieved due to the increased charge density of the lamellar nanochannels and the excellent aqueous stability of the membranes.
35027125	5	51	theme	nanochannels	954:965	arg1	density					930:936	the increased charge density	909:936	the increased charge density of the lamellar nanochannels	909:965	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl) was achieved due to the increased charge density of the lamellar nanochannels and the excellent aqueous stability of the membranes.
35027125	5	52	theme	0.014 S cm-1	840:851	arg1	conductivity					824:835	Higher ion conductivity	813:835	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl)	813:887	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl) was achieved due to the increased charge density of the lamellar nanochannels and the excellent aqueous stability of the membranes.
35027125	3	53	theme	vacuum	643:648	arg1	filtration					650:659	vacuum filtration	643:659	vacuum filtration	643:659	Composite membranes exhibiting charge-governed ion conductivity were prepared via co-homogenization of g-C3N4 with CNF and vacuum filtration.
35027125	5	54	theme	aqueous	985:991	arg1	stability					993:1001	the excellent aqueous stability	971:1001	the excellent aqueous stability of the membranes	971:1018	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl) was achieved due to the increased charge density of the lamellar nanochannels and the excellent aqueous stability of the membranes.
35027125	0	55	theme	energy	51:56	arg1	conversion					58:67	high-efficient osmotic energy conversion	28:67	high-efficient osmotic energy conversion	28:67	Increased ion transport and high-efficient osmotic energy conversion through aqueous stable graphitic carbon nitride/cellulose nanofiber composite membrane.
35027125	2	56	theme	nitride	367:373	arg1	membranes					420:428	graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes	350:428	graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels	350:464	Herein, we fabricate graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels for capturing osmotic energy from salinity gradients.
35027125	7	57	theme	improved	1251:1258	arg1	conversion					1275:1284	improved osmotic energy conversion	1251:1284	improved osmotic energy conversion	1251:1284	This work could inspire new designs of cellulose-based nanofluidic devices for improved osmotic energy conversion.
35027125	4	58	theme	weight	763:768	arg1	content					770:776	the weight content	759:776	the weight content of CNF in the composite membranes	759:810	Ion conductivity was efficiently modulated by fine-tuning the charge density through controlling the weight content of CNF in the composite membranes.
35027125	1	59	theme	Increased	157:165	arg1	attention					167:175	Increased attention	157:175	Increased attention	157:175	Increased attention has evoked on the utilization of renewable energy, particularly osmotic power as a potential solution to the energy crisis and environmental pollution.
35027125	6	60	theme	membranes	1067:1075	arg1	potential					1040:1048	the potential	1036:1048	the potential of the composite membranes in nanofluidic osmotic energy conversion	1036:1116	We demonstrate the potential of the composite membranes in nanofluidic osmotic energy conversion, displaying thermo-enhanced power output performance.
35027125	5	61	theme	low	856:858	arg1	concentrations					860:873	low concentrations	856:873	low concentrations (<10-2 M KCl)	856:887	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl) was achieved due to the increased charge density of the lamellar nanochannels and the excellent aqueous stability of the membranes.
35027125	5	61	theme	low	856:858	arg1	KCl					884:886	<10-2 M KCl	876:886	<10-2 M KCl	876:886	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl) was achieved due to the increased charge density of the lamellar nanochannels and the excellent aqueous stability of the membranes.
35027125	2	62	theme	graphitic	350:358	arg1	membranes					420:428	graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes	350:428	graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels	350:464	Herein, we fabricate graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels for capturing osmotic energy from salinity gradients.
35027125	2	63	theme	carbon	360:365	arg1	membranes					420:428	graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes	350:428	graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels	350:464	Herein, we fabricate graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels for capturing osmotic energy from salinity gradients.
35027125	0	64	theme	aqueous	77:83	arg1	nitride/cellulose					109:125	aqueous stable graphitic carbon nitride/cellulose	77:125	aqueous stable graphitic carbon nitride/cellulose	77:125	Increased ion transport and high-efficient osmotic energy conversion through aqueous stable graphitic carbon nitride/cellulose nanofiber composite membrane.
35027125	6	65	theme	output	1152:1157	arg1	performance					1159:1169	thermo-enhanced power output performance	1130:1169	thermo-enhanced power output performance	1130:1169	We demonstrate the potential of the composite membranes in nanofluidic osmotic energy conversion, displaying thermo-enhanced power output performance.
35027125	2	66	theme	nanofiber	394:402	arg1	membranes					420:428	graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes	350:428	graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels	350:464	Herein, we fabricate graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels for capturing osmotic energy from salinity gradients.
35027125	1	67	theme	potential	260:268	arg1	solution					270:277	a potential solution	258:277	a potential solution to the energy crisis and environmental pollution	258:326	Increased attention has evoked on the utilization of renewable energy, particularly osmotic power as a potential solution to the energy crisis and environmental pollution.
35027125	4	68	theme	Ion	662:664	arg1	conductivity					666:677	Ion conductivity	662:677	Ion conductivity	662:677	Ion conductivity was efficiently modulated by fine-tuning the charge density through controlling the weight content of CNF in the composite membranes.
35027125	6	69	theme	osmotic	1092:1098	arg1	conversion					1107:1116	nanofluidic osmotic energy conversion	1080:1116	nanofluidic osmotic energy conversion	1080:1116	We demonstrate the potential of the composite membranes in nanofluidic osmotic energy conversion, displaying thermo-enhanced power output performance.
35027125	2	70	theme	/cellulose	383:392	arg1	membranes					420:428	graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes	350:428	graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels	350:464	Herein, we fabricate graphitic carbon nitride (g-C3N4)/cellulose nanofiber (CNF) composite membranes with tailored lamellar nanochannels for capturing osmotic energy from salinity gradients.
35027125	5	71	theme	Higher	813:818	arg1	conductivity					824:835	Higher ion conductivity	813:835	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl)	813:887	Higher ion conductivity of 0.014 S cm-1 at low concentrations (<10-2 M KCl) was achieved due to the increased charge density of the lamellar nanochannels and the excellent aqueous stability of the membranes.
34687763	1	0	dep	new	273:275	arg1	green					278:282	green	278:282	green	278:282	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields is important for the sustainable development of new, green, light-weight, functional biomass materials.
34687763	1	0	dep	new	273:275	arg1	functional					299:308	functional	299:308	functional	299:308	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields is important for the sustainable development of new, green, light-weight, functional biomass materials.
34687763	1	0	dep	new	273:275	arg1	light-weight					285:296	light-weight	285:296	light-weight	285:296	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields is important for the sustainable development of new, green, light-weight, functional biomass materials.
34687763	1	1	from	fibers	172:177	arg1	fields					218:223	other fields	212:223	other fields	212:223	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields is important for the sustainable development of new, green, light-weight, functional biomass materials.
34687763	1	1	from	fibers	172:177	arg1	composites					196:205	natural composites	188:205	natural composites	188:205	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields is important for the sustainable development of new, green, light-weight, functional biomass materials.
34687763	1	2	theme	prospects	151:159	arg1	Exploration					120:130	Exploration	120:130	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields	120:223	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields is important for the sustainable development of new, green, light-weight, functional biomass materials.
34687763	4	3	theme	microscopy	541:550	arg1	results					508:514	The results	504:514	The results of transmission electron microscopy	504:550	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	4	3	theme	microscopy	541:550	arg1	data					572:575	tensile testing data	556:575	tensile testing data	556:575	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	3	4	contain	have	469:472	arg2	density					480:486	a low density	474:486	a low density (618.0 kg m-3)	474:501	The CFs have a low density (618.0 kg m-3).
34687763	3	4	contain	have	469:472	arg1	CFs					465:467	The CFs	461:467	The CFs	461:467	The CFs have a low density (618.0 kg m-3).
34687763	3	4	contain	have	469:472	arg2	618.0 kg m-3					489:500	618.0 kg m-3	489:500	618.0 kg m-3	489:500	The CFs have a low density (618.0 kg m-3).
34687763	1	5	theme	biomass	310:316	arg1	materials					318:326	new, green, light-weight, functional biomass materials	273:326	new, green, light-weight, functional biomass materials	273:326	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields is important for the sustainable development of new, green, light-weight, functional biomass materials.
34687763	1	6	from	prospects	151:159	arg1	fields					218:223	other fields	212:223	other fields	212:223	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields is important for the sustainable development of new, green, light-weight, functional biomass materials.
34687763	1	6	from	prospects	151:159	arg1	composites					196:205	natural composites	188:205	natural composites	188:205	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields is important for the sustainable development of new, green, light-weight, functional biomass materials.
34687763	5	7	theme	CBF	811:813	arg1	bundle					816:821	the cattail branch fiber (CBF) bundle	785:821	the cattail branch fiber (CBF) bundle	785:821	In turn, the cattail branch fiber (CBF) bundle is composed of parenchyma cells with specific "half-honeycomb" shape.
34687763	4	8	theme	electron	532:539	arg1	microscopy					541:550	transmission electron microscopy	519:550	transmission electron microscopy	519:550	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	1	9	theme	materials	318:326	arg1	development					258:268	the sustainable development	242:268	the sustainable development of new, green, light-weight, functional biomass materials	242:326	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields is important for the sustainable development of new, green, light-weight, functional biomass materials.
34687763	8	10	theme	infrared	1120:1127	arg1	analyses					1142:1149	infrared spectroscopy analyses	1120:1149	infrared spectroscopy analyses	1120:1149	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	5	11	theme	fiber	804:808	arg1	bundle					816:821	the cattail branch fiber (CBF) bundle	785:821	the cattail branch fiber (CBF) bundle	785:821	In turn, the cattail branch fiber (CBF) bundle is composed of parenchyma cells with specific "half-honeycomb" shape.
34687763	4	12	theme	CTF	617:619	arg1	bundle					622:627	the cattail trunk fiber (CTF) bundle	592:627	the cattail trunk fiber (CTF) bundle	592:627	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	4	13	theme	tensile	556:562	arg1	data					572:575	tensile testing data	556:575	tensile testing data	556:575	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	0	14	theme	micro/nano	77:86	arg1	structure					88:96	micro/nano structure	77:96	micro/nano structure	77:96	Characterization of potential cellulose fiber from cattail fiber: A study on micro/nano structure and other properties.
34687763	0	15	from	fiber	59:63	arg1	Characterization					0:15	Characterization	0:15	Characterization of potential cellulose fiber from cattail fiber: A study on micro/nano structure and other properties.	0:118	Characterization of potential cellulose fiber from cattail fiber: A study on micro/nano structure and other properties.
34687763	4	16	theme	specific	703:710	arg1	modulus					712:718	high specific modulus	698:718	high specific modulus (10.1 MPa∙m3·kg-1)	698:737	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	4	16	theme	specific	703:710	arg1	10.1 MPa∙m3·kg-1					721:736	10.1 MPa∙m3·kg-1	721:736	10.1 MPa∙m3·kg-1	721:736	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	8	17	theme	composites	1528:1537	arg1	processability					1495:1508	processability	1495:1508	processability of CFs-reinforced composites	1495:1537	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	4	18	theme	parenchyma	644:653	arg1	cells					655:659	parenchyma cells	644:659	parenchyma cells	644:659	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	5	19	with	cells	849:853	arg1	shape					886:890	specific "half-honeycomb" shape	860:890	specific "half-honeycomb" shape	860:890	In turn, the cattail branch fiber (CBF) bundle is composed of parenchyma cells with specific "half-honeycomb" shape.
34687763	0	20	theme	other	102:106	arg1	properties					108:117	other properties	102:117	other properties	102:117	Characterization of potential cellulose fiber from cattail fiber: A study on micro/nano structure and other properties.
34687763	7	21	theme	CTF	992:994	arg1	bundles					996:1002	the CTF bundles	988:1002	the CTF bundles	988:1002	This structural feature endows the CTF bundles with stable structure, good oil absorption and storage capacities.
34687763	6	22	theme	open	942:945	arg1	cavities					947:954	the open cavities	938:954	the open cavities	938:954	The inner diaphragms divide these cells into the open cavities.
34687763	5	23	theme	half-honeycomb	870:883	arg1	shape					886:890	specific "half-honeycomb" shape	860:890	specific "half-honeycomb" shape	860:890	In turn, the cattail branch fiber (CBF) bundle is composed of parenchyma cells with specific "half-honeycomb" shape.
34687763	1	24	theme	cattail	164:170	arg1	CFs					180:182	CFs	180:182	CFs	180:182	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields is important for the sustainable development of new, green, light-weight, functional biomass materials.
34687763	1	24	theme	cattail	164:170	arg1	fibers					172:177	cattail fibers	164:177	cattail fibers (CFs) in natural composites, and other fields	164:223	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields is important for the sustainable development of new, green, light-weight, functional biomass materials.
34687763	7	25	theme	oil	1032:1034	arg1	absorption					1036:1045	good oil absorption	1027:1045	good oil absorption	1027:1045	This structural feature endows the CTF bundles with stable structure, good oil absorption and storage capacities.
34687763	8	26	theme	degradation	1419:1429	arg1	temperature					1431:1441	its final degradation temperature	1409:1441	its final degradation temperature	1409:1441	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	6	27	theme	inner	897:901	arg1	diaphragms					903:912	The inner diaphragms	893:912	The inner diaphragms	893:912	The inner diaphragms divide these cells into the open cavities.
34687763	8	28	dep	resistance	1281:1290	arg1	CF.					1350:1352	CF.	1350:1352	CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites	1350:1537	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	5	29	theme	"	884:884	arg1	shape					886:890	specific "half-honeycomb" shape	860:890	specific "half-honeycomb" shape	860:890	In turn, the cattail branch fiber (CBF) bundle is composed of parenchyma cells with specific "half-honeycomb" shape.
34687763	1	30	theme	fibers	172:177	arg1	prospects					151:159	the application prospects	135:159	the application prospects of cattail fibers (CFs) in natural composites, and other fields	135:223	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields is important for the sustainable development of new, green, light-weight, functional biomass materials.
34687763	2	31	theme	mechanical	408:417	arg1	characteristics					419:433	mechanical characteristics	408:433	mechanical characteristics	408:433	In this study, the physical and chemical properties, micro/nano structure, and mechanical characteristics of CFs were investigated.
34687763	8	32	theme	corrosion	1271:1279	arg1	resistance					1281:1290	corrosion resistance	1271:1290	corrosion resistance	1271:1290	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	4	33	theme	fiber	610:614	arg1	bundle					622:627	the cattail trunk fiber (CTF) bundle	592:627	the cattail trunk fiber (CTF) bundle	592:627	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	8	34	theme	chemical	1075:1082	arg1	component					1084:1092	The chemical component	1071:1092	The chemical component	1071:1092	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	1	35	theme	sustainable	246:256	arg1	development					258:268	the sustainable development	242:268	the sustainable development of new, green, light-weight, functional biomass materials	242:326	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields is important for the sustainable development of new, green, light-weight, functional biomass materials.
34687763	5	36	theme	parenchyma	838:847	arg1	cells					849:853	parenchyma cells	838:853	parenchyma cells with specific "half-honeycomb" shape	838:890	In turn, the cattail branch fiber (CBF) bundle is composed of parenchyma cells with specific "half-honeycomb" shape.
34687763	5	37	theme	cattail	789:795	arg1	bundle					816:821	the cattail branch fiber (CBF) bundle	785:821	the cattail branch fiber (CBF) bundle	785:821	In turn, the cattail branch fiber (CBF) bundle is composed of parenchyma cells with specific "half-honeycomb" shape.
34687763	0	38	theme	cellulose	30:38	arg1	fiber					40:44	potential cellulose fiber	20:44	potential cellulose fiber	20:44	Characterization of potential cellulose fiber from cattail fiber: A study on micro/nano structure and other properties.
34687763	7	39	theme	structural	962:971	arg1	feature					973:979	This structural feature	957:979	This structural feature	957:979	This structural feature endows the CTF bundles with stable structure, good oil absorption and storage capacities.
34687763	4	40	theme	high	698:701	arg1	modulus					712:718	high specific modulus	698:718	high specific modulus (10.1 MPa∙m3·kg-1)	698:737	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	4	40	theme	high	698:701	arg1	10.1 MPa∙m3·kg-1					721:736	10.1 MPa∙m3·kg-1	721:736	10.1 MPa∙m3·kg-1	721:736	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	2	41	theme	micro/nano	382:391	arg1	structure					393:401	micro/nano structure	382:401	micro/nano structure	382:401	In this study, the physical and chemical properties, micro/nano structure, and mechanical characteristics of CFs were investigated.
34687763	0	42	theme	potential	20:28	arg1	fiber					40:44	potential cellulose fiber	20:44	potential cellulose fiber	20:44	Characterization of potential cellulose fiber from cattail fiber: A study on micro/nano structure and other properties.
34687763	4	43	theme	transmission	519:530	arg1	microscopy					541:550	transmission electron microscopy	519:550	transmission electron microscopy	519:550	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	8	44	theme	wax	1208:1210	arg1	%					1225:1225	11.5%	1221:1225	11.5%	1221:1225	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	8	44	theme	wax	1208:1210	arg1	content					1212:1218	wax content	1208:1218	wax content (11.5%)	1208:1226	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	5	45	theme	branch	797:802	arg1	bundle					816:821	the cattail branch fiber (CBF) bundle	785:821	the cattail branch fiber (CBF) bundle	785:821	In turn, the cattail branch fiber (CBF) bundle is composed of parenchyma cells with specific "half-honeycomb" shape.
34687763	1	46	theme	new	273:275	arg1	materials					318:326	new, green, light-weight, functional biomass materials	273:326	new, green, light-weight, functional biomass materials	273:326	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields is important for the sustainable development of new, green, light-weight, functional biomass materials.
34687763	8	47	from	increase	1483:1490	arg1	processability					1495:1508	processability	1495:1508	processability of CFs-reinforced composites	1495:1537	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	0	48	from	study	68:72	arg1	structure					88:96	micro/nano structure	77:96	micro/nano structure	77:96	Characterization of potential cellulose fiber from cattail fiber: A study on micro/nano structure and other properties.
34687763	0	48	from	study	68:72	arg1	properties					108:117	other properties	102:117	other properties	102:117	Characterization of potential cellulose fiber from cattail fiber: A study on micro/nano structure and other properties.
34687763	8	49	theme	CFs-reinforced	1513:1526	arg1	composites					1528:1537	CFs-reinforced composites	1513:1537	CFs-reinforced composites	1513:1537	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	1	50	theme	natural	188:194	arg1	composites					196:205	natural composites	188:205	natural composites	188:205	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields is important for the sustainable development of new, green, light-weight, functional biomass materials.
34687763	0	51	theme	fiber	40:44	arg1	Characterization					0:15	Characterization	0:15	Characterization of potential cellulose fiber from cattail fiber: A study on micro/nano structure and other properties.	0:118	Characterization of potential cellulose fiber from cattail fiber: A study on micro/nano structure and other properties.
34687763	2	52	theme	CFs	438:440	arg1	properties					370:379	the physical and chemical properties	344:379	the physical and chemical properties	344:379	In this study, the physical and chemical properties, micro/nano structure, and mechanical characteristics of CFs were investigated.
34687763	2	52	theme	CFs	438:440	arg1	structure					393:401	micro/nano structure	382:401	micro/nano structure	382:401	In this study, the physical and chemical properties, micro/nano structure, and mechanical characteristics of CFs were investigated.
34687763	2	52	theme	CFs	438:440	arg1	characteristics					419:433	mechanical characteristics	408:433	mechanical characteristics	408:433	In this study, the physical and chemical properties, micro/nano structure, and mechanical characteristics of CFs were investigated.
34687763	8	53	contain	have	1169:1172	arg2	content					1212:1218	wax content	1208:1218	wax content (11.5%)	1208:1226	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	8	53	contain	have	1169:1172	arg2	content					1188:1194	higher lignin content	1174:1194	higher lignin content (20.6%)	1174:1202	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	8	53	contain	have	1169:1172	arg2	%					1201:1201	20.6%	1197:1201	20.6%	1197:1201	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	8	53	contain	have	1169:1172	arg1	CFs					1165:1167	the CFs	1161:1167	the CFs	1161:1167	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	8	53	contain	have	1169:1172	arg2	%					1225:1225	11.5%	1221:1225	11.5%	1221:1225	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	7	54	theme	storage	1051:1057	arg1	capacities					1059:1068	storage capacities	1051:1068	storage capacities	1051:1068	This structural feature endows the CTF bundles with stable structure, good oil absorption and storage capacities.
34687763	2	55	theme	chemical	361:368	arg1	properties					370:379	the physical and chemical properties	344:379	the physical and chemical properties	344:379	In this study, the physical and chemical properties, micro/nano structure, and mechanical characteristics of CFs were investigated.
34687763	7	56	theme	good	1027:1030	arg1	absorption					1036:1045	good oil absorption	1027:1045	good oil absorption	1027:1045	This structural feature endows the CTF bundles with stable structure, good oil absorption and storage capacities.
34687763	8	57	theme	final	1413:1417	arg1	temperature					1431:1441	its final degradation temperature	1409:1441	its final degradation temperature	1409:1441	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	8	58	theme	lipophilic-hydrophobic	1315:1336	arg1	property					1338:1345	lipophilic-hydrophobic property	1315:1345	lipophilic-hydrophobic property	1315:1345	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	1	59	from	composites	196:205	arg1	prospects					151:159	the application prospects	135:159	the application prospects of cattail fibers (CFs) in natural composites, and other fields	135:223	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields is important for the sustainable development of new, green, light-weight, functional biomass materials.
34687763	0	60	theme	cattail	51:57	arg1	fiber					59:63	cattail fiber	51:63	cattail fiber	51:63	Characterization of potential cellulose fiber from cattail fiber: A study on micro/nano structure and other properties.
34687763	1	61	from	fields	218:223	arg1	prospects					151:159	the application prospects	135:159	the application prospects of cattail fibers (CFs) in natural composites, and other fields	135:223	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields is important for the sustainable development of new, green, light-weight, functional biomass materials.
34687763	4	62	from	break	762:766	arg1	modulus					712:718	high specific modulus	698:718	high specific modulus (10.1 MPa∙m3·kg-1)	698:737	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	4	62	from	break	762:766	arg1	%					772:772	3.9%	769:772	3.9%	769:772	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	4	62	from	break	762:766	arg1	elongation					748:757	high elongation	743:757	high elongation at break (3.9%)	743:773	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	4	62	from	break	762:766	arg1	10.1 MPa∙m3·kg-1					721:736	10.1 MPa∙m3·kg-1	721:736	10.1 MPa∙m3·kg-1	721:736	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	8	63	theme	property	1338:1345	arg1	improvement					1256:1266	the improvement	1252:1266	the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites	1252:1537	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	8	64	theme	stability	1301:1309	arg1	improvement					1256:1266	the improvement	1252:1266	the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites	1252:1537	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	4	65	theme	high	743:746	arg1	%					772:772	3.9%	769:772	3.9%	769:772	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	4	65	theme	high	743:746	arg1	elongation					748:757	high elongation	743:757	high elongation at break (3.9%)	743:773	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	8	66	theme	lignin	1181:1186	arg1	content					1188:1194	higher lignin content	1174:1194	higher lignin content (20.6%)	1174:1202	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	8	66	theme	lignin	1181:1186	arg1	%					1201:1201	20.6%	1197:1201	20.6%	1197:1201	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	8	67	theme	resistance	1281:1290	arg1	improvement					1256:1266	the improvement	1252:1266	the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites	1252:1537	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	4	68	theme	stone	671:675	arg1	cells					677:681	solid stone cells	665:681	solid stone cells	665:681	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	8	69	theme	higher	1174:1179	arg1	content					1188:1194	higher lignin content	1174:1194	higher lignin content (20.6%)	1174:1202	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	8	69	theme	higher	1174:1179	arg1	%					1201:1201	20.6%	1197:1201	20.6%	1197:1201	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	8	70	dep	Fourier	1102:1108	arg1	transform					1110:1118	transform	1110:1118	transform infrared spectroscopy analyses	1110:1149	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	5	71	theme	specific	860:867	arg1	shape					886:890	specific "half-honeycomb" shape	860:890	specific "half-honeycomb" shape	860:890	In turn, the cattail branch fiber (CBF) bundle is composed of parenchyma cells with specific "half-honeycomb" shape.
34687763	4	72	theme	solid	665:669	arg1	cells					677:681	solid stone cells	665:681	solid stone cells	665:681	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	4	73	theme	testing	564:570	arg1	data					572:575	tensile testing data	556:575	tensile testing data	556:575	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	4	74	theme	trunk	604:608	arg1	bundle					622:627	the cattail trunk fiber (CTF) bundle	592:627	the cattail trunk fiber (CTF) bundle	592:627	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	8	75	theme	thermal	1293:1299	arg1	stability					1301:1309	thermal stability	1293:1309	thermal stability	1293:1309	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	4	76	theme	cattail	596:602	arg1	bundle					622:627	the cattail trunk fiber (CTF) bundle	592:627	the cattail trunk fiber (CTF) bundle	592:627	The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%).
34687763	3	77	theme	low	476:478	arg1	618.0 kg m-3					489:500	618.0 kg m-3	489:500	618.0 kg m-3	489:500	The CFs have a low density (618.0 kg m-3).
34687763	3	77	theme	low	476:478	arg1	density					480:486	a low density	474:486	a low density (618.0 kg m-3)	474:501	The CFs have a low density (618.0 kg m-3).
34687763	2	78	theme	physical	348:355	arg1	properties					370:379	the physical and chemical properties	344:379	the physical and chemical properties	344:379	In this study, the physical and chemical properties, micro/nano structure, and mechanical characteristics of CFs were investigated.
34687763	1	79	theme	other	212:216	arg1	fields					218:223	other fields	212:223	other fields	212:223	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields is important for the sustainable development of new, green, light-weight, functional biomass materials.
34687763	8	80	theme	spectroscopy	1129:1140	arg1	analyses					1142:1149	infrared spectroscopy analyses	1120:1149	infrared spectroscopy analyses	1120:1149	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	8	81	theme	thermogravimetric	1367:1383	arg1	analysis					1385:1392	the thermogravimetric analysis	1363:1392	the thermogravimetric analysis	1363:1392	The chemical component and the Fourier transform infrared spectroscopy analyses show that the CFs have higher lignin content (20.6%) and wax content (11.5%), which are conducive to the improvement of corrosion resistance, thermal stability and lipophilic-hydrophobic property of CF. Finally, the thermogravimetric analysis indicates that its final degradation temperature is 404.5 °C, which is beneficial to the increase in processability of CFs-reinforced composites.
34687763	0	82	dep	Characterization	0:15	arg1	study					68:72	A study	66:72	Characterization of potential cellulose fiber from cattail fiber: A study on micro/nano structure and other properties.	0:118	Characterization of potential cellulose fiber from cattail fiber: A study on micro/nano structure and other properties.
34687763	1	83	theme	application	139:149	arg1	prospects					151:159	the application prospects	135:159	the application prospects of cattail fibers (CFs) in natural composites, and other fields	135:223	Exploration of the application prospects of cattail fibers (CFs) in natural composites, and other fields is important for the sustainable development of new, green, light-weight, functional biomass materials.
34687763	7	84	theme	stable	1009:1014	arg1	structure					1016:1024	stable structure	1009:1024	stable structure	1009:1024	This structural feature endows the CTF bundles with stable structure, good oil absorption and storage capacities.
34465434	2	0	theme	Cr	551:552	arg1	removal					540:546	the flocculation removal	523:546	the flocculation removal of Cr(III), Co(II), and Pb(II)	523:577	They were applied to the flocculation removal of Cr(III), Co(II), and Pb(II).
34465434	1	1	theme	chitosan	394:401	arg1	flocculant					403:412	2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant	304:412	2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA)	304:419	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	1	1	theme	chitosan	394:401	arg1	MCAA					415:418	MCAA	415:418	MCAA	415:418	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	4	2	theme	magnetic	825:832	arg1	properties					844:853	good magnetic induction properties	820:853	good magnetic induction properties	820:853	Characterization results show that the three magnetic carboxymethyl chitosan-based flocculants have been successfully prepared with good magnetic induction properties.
34465434	5	3	theme	magnetic	1105:1112	arg1	strength					1120:1127	precipitation magnetic field strength	1091:1127	precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively	1091:1232	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	1	4	theme	carboxymethyl	259:271	arg1	MCM					294:296	MCM	294:296	MCM	294:296	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	1	4	theme	carboxymethyl	259:271	arg1	flocculant					282:291	acrylamide-grafted magnetic carboxymethyl chitosan flocculant	231:291	acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM)	231:297	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	5	5	theme	80 mg/L	1007:1013	arg1	flocculant					1015:1024	80 mg/L flocculant	1007:1024	80 mg/L flocculant	1007:1024	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	3	6	theme	Co	656:657	arg1	performance					632:642	the removal performance	620:642	the removal performance of Cr(III), Co(II), and Pb(II)	620:673	The effect of flocculation condition on the removal performance of Cr(III), Co(II), and Pb(II) was studied.
34465434	4	7	theme	good	820:823	arg1	properties					844:853	good magnetic induction properties	820:853	good magnetic induction properties	820:853	Characterization results show that the three magnetic carboxymethyl chitosan-based flocculants have been successfully prepared with good magnetic induction properties.
34465434	5	8	theme	field	1114:1118	arg1	strength					1120:1127	precipitation magnetic field strength	1091:1127	precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively	1091:1232	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	1	9	theme	chitosan	273:280	arg1	MCM					294:296	MCM	294:296	MCM	294:296	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	1	9	theme	chitosan	273:280	arg1	flocculant					282:291	acrylamide-grafted magnetic carboxymethyl chitosan flocculant	231:291	acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM)	231:297	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	9	10	theme	technology	1647:1656	arg1	development					1596:1606	the development	1592:1606	the development of efficient and low-cost flocculation technology	1592:1656	This research provides guidance and ideas for the development of efficient and low-cost flocculation technology to remove heavy metals in wastewater.
34465434	4	11	theme	Characterization	688:703	arg1	results					705:711	Characterization results	688:711	Characterization results	688:711	Characterization results show that the three magnetic carboxymethyl chitosan-based flocculants have been successfully prepared with good magnetic induction properties.
34465434	5	12	theme	G	1067:1067	arg1	value					1069:1073	G value	1067:1073	G value of 200 s-1	1067:1084	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	2	13	theme	Co	560:561	arg1	removal					540:546	the flocculation removal	523:546	the flocculation removal of Cr(III), Co(II), and Pb(II)	523:577	They were applied to the flocculation removal of Cr(III), Co(II), and Pb(II).
34465434	3	14	theme	Pb	668:669	arg1	performance					632:642	the removal performance	620:642	the removal performance of Cr(III), Co(II), and Pb(II)	620:673	The effect of flocculation condition on the removal performance of Cr(III), Co(II), and Pb(II) was studied.
34465434	4	15	theme	induction	834:842	arg1	properties					844:853	good magnetic induction properties	820:853	good magnetic induction properties	820:853	Characterization results show that the three magnetic carboxymethyl chitosan-based flocculants have been successfully prepared with good magnetic induction properties.
34465434	5	16	theme	Co	1161:1162	arg1	rates					1152:1156	The removal rates	1140:1156	The removal rates of Co(II) by MC, MCM, and MCAA	1140:1187	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	16	theme	Co	1161:1162	arg1	%					1198:1198	54.33%	1193:1198	54.33%	1193:1198	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	7	17	theme	metal-simulated	1390:1404	arg1	wastewater					1406:1415	composite heavy metal-simulated wastewater	1374:1415	composite heavy metal-simulated wastewater	1374:1415	MCAA shows good flocculation performance in composite heavy metal-simulated wastewater.
34465434	8	18	theme	magnetic	1422:1429	arg1	flocculant					1460:1469	The magnetic carboxymethyl chitosan-based flocculant	1418:1469	The magnetic carboxymethyl chitosan-based flocculant	1418:1469	The magnetic carboxymethyl chitosan-based flocculant shows excellent flocculation performance in removing soluble heavy metals.
34465434	1	19	with	flocculants	123:133	arg1	MCAA					415:418	MCAA	415:418	MCAA	415:418	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	1	19	with	flocculants	123:133	arg1	MCM					294:296	MCM	294:296	MCM	294:296	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	1	19	with	flocculants	123:133	arg1	groups					160:165	different chelating groups	140:165	different chelating groups	140:165	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	1	19	with	flocculants	123:133	arg1	flocculant					214:223	carboxymethyl chitosan-modified Fe3O4 flocculant	176:223	carboxymethyl chitosan-modified Fe3O4 flocculant (MC)	176:228	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	1	19	with	flocculants	123:133	arg1	MC					226:227	MC	226:227	MC	226:227	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	1	19	with	flocculants	123:133	arg1	flocculant					403:412	2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant	304:412	2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA)	304:419	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	1	19	with	flocculants	123:133	arg1	flocculant					282:291	acrylamide-grafted magnetic carboxymethyl chitosan flocculant	231:291	acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM)	231:297	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	5	20	dep	120 mT.	1132:1138	arg1	%					1206:1206	84.99%	1201:1206	84.99%	1201:1206	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	20	dep	120 mT.	1132:1138	arg1	rates					1152:1156	The removal rates	1140:1156	The removal rates of Co(II) by MC, MCM, and MCAA	1140:1187	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	20	dep	120 mT.	1132:1138	arg1	%					1218:1218	90.49%	1213:1218	90.49%	1213:1218	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	20	dep	120 mT.	1132:1138	arg1	%					1198:1198	54.33%	1193:1198	54.33%	1193:1198	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	21	theme	MCAA	921:924	arg1	%					946:946	51.79%	941:946	51.79%	941:946	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	21	theme	MCAA	921:924	arg1	rates					899:903	the removal rates	887:903	the removal rates of MC, MCM, and MCAA on Cr(III)	887:935	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	9	22	theme	heavy	1668:1672	arg1	metals					1674:1679	heavy metals	1668:1679	heavy metals in wastewater	1668:1693	This research provides guidance and ideas for the development of efficient and low-cost flocculation technology to remove heavy metals in wastewater.
34465434	0	23	from	solutions	33:41	arg1	removal					12:18	Heavy metal removal	0:18	Heavy metal removal from aqueous solutions by chitosan-based magnetic composite	0:78	Heavy metal removal from aqueous solutions by chitosan-based magnetic composite flocculants.
34465434	5	24	theme	1.5 hr	1059:1064	arg1	strength					1120:1127	precipitation magnetic field strength	1091:1127	precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively	1091:1232	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	24	theme	1.5 hr	1059:1064	arg1	value					1069:1073	G value	1067:1073	G value of 200 s-1	1067:1084	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	24	theme	1.5 hr	1059:1064	arg1	conditions					993:1002	the conditions	989:1002	the conditions of 80 mg/L flocculant	989:1024	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	24	theme	1.5 hr	1059:1064	arg1	time					1051:1054	reaction time	1042:1054	reaction time of 1.5 hr	1042:1064	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	24	theme	1.5 hr	1059:1064	arg1	value					1030:1034	pH value	1027:1034	pH value of 6	1027:1039	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	7	25	theme	composite	1374:1382	arg1	wastewater					1406:1415	composite heavy metal-simulated wastewater	1374:1415	composite heavy metal-simulated wastewater	1374:1415	MCAA shows good flocculation performance in composite heavy metal-simulated wastewater.
34465434	0	26	theme	metal	6:10	arg1	removal					12:18	Heavy metal removal	0:18	Heavy metal removal from aqueous solutions by chitosan-based magnetic composite	0:78	Heavy metal removal from aqueous solutions by chitosan-based magnetic composite flocculants.
34465434	6	27	theme	Pb	1256:1257	arg1	%					1293:1293	61.54%	1288:1293	61.54%	1288:1293	The removal rates of Pb(II) by MC, MCM, and MCAA are 61.54%, 91.32%, and 95.74%, respectively.
34465434	6	27	theme	Pb	1256:1257	arg1	rates					1247:1251	The removal rates	1235:1251	The removal rates of Pb(II) by MC, MCM, and MCAA	1235:1282	The removal rates of Pb(II) by MC, MCM, and MCAA are 61.54%, 91.32%, and 95.74%, respectively.
34465434	5	28	from	rates	899:903	arg1	Cr					929:930	Cr(III)	929:935	Cr(III)	929:935	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	7	29	theme	heavy	1384:1388	arg1	wastewater					1406:1415	composite heavy metal-simulated wastewater	1374:1415	composite heavy metal-simulated wastewater	1374:1415	MCAA shows good flocculation performance in composite heavy metal-simulated wastewater.
34465434	1	30	theme	carboxymethyl	176:188	arg1	flocculant					214:223	carboxymethyl chitosan-modified Fe3O4 flocculant	176:223	carboxymethyl chitosan-modified Fe3O4 flocculant (MC)	176:228	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	1	30	theme	carboxymethyl	176:188	arg1	MC					226:227	MC	226:227	MC	226:227	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	0	31	theme	Heavy	0:4	arg1	removal					12:18	Heavy metal removal	0:18	Heavy metal removal from aqueous solutions by chitosan-based magnetic composite	0:78	Heavy metal removal from aqueous solutions by chitosan-based magnetic composite flocculants.
34465434	5	32	theme	MC	908:909	arg1	%					946:946	51.79%	941:946	51.79%	941:946	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	32	theme	MC	908:909	arg1	rates					899:903	the removal rates	887:903	the removal rates of MC, MCM, and MCAA on Cr(III)	887:935	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	1	33	theme	chitosan-modified	190:206	arg1	flocculant					214:223	carboxymethyl chitosan-modified Fe3O4 flocculant	176:223	carboxymethyl chitosan-modified Fe3O4 flocculant (MC)	176:228	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	1	33	theme	chitosan-modified	190:206	arg1	MC					226:227	MC	226:227	MC	226:227	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	4	34	theme	magnetic	733:740	arg1	flocculants					771:781	the three magnetic carboxymethyl chitosan-based flocculants	723:781	the three magnetic carboxymethyl chitosan-based flocculants	723:781	Characterization results show that the three magnetic carboxymethyl chitosan-based flocculants have been successfully prepared with good magnetic induction properties.
34465434	8	35	theme	chitosan-based	1445:1458	arg1	flocculant					1460:1469	The magnetic carboxymethyl chitosan-based flocculant	1418:1469	The magnetic carboxymethyl chitosan-based flocculant	1418:1469	The magnetic carboxymethyl chitosan-based flocculant shows excellent flocculation performance in removing soluble heavy metals.
34465434	1	36	theme	Fe3O4	208:212	arg1	flocculant					214:223	carboxymethyl chitosan-modified Fe3O4 flocculant	176:223	carboxymethyl chitosan-modified Fe3O4 flocculant (MC)	176:228	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	1	36	theme	Fe3O4	208:212	arg1	MC					226:227	MC	226:227	MC	226:227	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	7	37	theme	good	1341:1344	arg1	performance					1359:1369	good flocculation performance	1341:1369	good flocculation performance	1341:1369	MCAA shows good flocculation performance in composite heavy metal-simulated wastewater.
34465434	9	38	theme	low-cost	1625:1632	arg1	technology					1647:1656	efficient and low-cost flocculation technology	1611:1656	efficient and low-cost flocculation technology	1611:1656	This research provides guidance and ideas for the development of efficient and low-cost flocculation technology to remove heavy metals in wastewater.
34465434	5	39	theme	MCM	912:914	arg1	%					946:946	51.79%	941:946	51.79%	941:946	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	39	theme	MCM	912:914	arg1	rates					899:903	the removal rates	887:903	the removal rates of MC, MCM, and MCAA on Cr(III)	887:935	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	3	40	from	effect	584:589	arg1	performance					632:642	the removal performance	620:642	the removal performance of Cr(III), Co(II), and Pb(II)	620:673	The effect of flocculation condition on the removal performance of Cr(III), Co(II), and Pb(II) was studied.
34465434	8	41	theme	excellent	1477:1485	arg1	performance					1500:1510	excellent flocculation performance	1477:1510	excellent flocculation performance	1477:1510	The magnetic carboxymethyl chitosan-based flocculant shows excellent flocculation performance in removing soluble heavy metals.
34465434	8	42	theme	carboxymethyl	1431:1443	arg1	flocculant					1460:1469	The magnetic carboxymethyl chitosan-based flocculant	1418:1469	The magnetic carboxymethyl chitosan-based flocculant	1418:1469	The magnetic carboxymethyl chitosan-based flocculant shows excellent flocculation performance in removing soluble heavy metals.
34465434	9	43	theme	flocculation	1634:1645	arg1	technology					1647:1656	efficient and low-cost flocculation technology	1611:1656	efficient and low-cost flocculation technology	1611:1656	This research provides guidance and ideas for the development of efficient and low-cost flocculation technology to remove heavy metals in wastewater.
34465434	3	44	theme	Cr	647:648	arg1	performance					632:642	the removal performance	620:642	the removal performance of Cr(III), Co(II), and Pb(II)	620:673	The effect of flocculation condition on the removal performance of Cr(III), Co(II), and Pb(II) was studied.
34465434	4	45	theme	chitosan-based	756:769	arg1	flocculants					771:781	the three magnetic carboxymethyl chitosan-based flocculants	723:781	the three magnetic carboxymethyl chitosan-based flocculants	723:781	Characterization results show that the three magnetic carboxymethyl chitosan-based flocculants have been successfully prepared with good magnetic induction properties.
34465434	7	46	theme	flocculation	1346:1357	arg1	performance					1359:1369	good flocculation performance	1341:1369	good flocculation performance	1341:1369	MCAA shows good flocculation performance in composite heavy metal-simulated wastewater.
34465434	0	47	theme	aqueous	25:31	arg1	solutions					33:41	aqueous solutions	25:41	aqueous solutions	25:41	Heavy metal removal from aqueous solutions by chitosan-based magnetic composite flocculants.
34465434	1	48	theme	2-acrylamide-2-methylpropanesulfonic	304:339	arg1	flocculant					403:412	2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant	304:412	2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA)	304:419	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	1	48	theme	2-acrylamide-2-methylpropanesulfonic	304:339	arg1	MCAA					415:418	MCAA	415:418	MCAA	415:418	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	2	49	theme	flocculation	527:538	arg1	removal					540:546	the flocculation removal	523:546	the flocculation removal of Cr(III), Co(II), and Pb(II)	523:577	They were applied to the flocculation removal of Cr(III), Co(II), and Pb(II).
34465434	3	50	theme	removal	624:630	arg1	performance					632:642	the removal performance	620:642	the removal performance of Cr(III), Co(II), and Pb(II)	620:673	The effect of flocculation condition on the removal performance of Cr(III), Co(II), and Pb(II) was studied.
34465434	4	51	theme	carboxymethyl	742:754	arg1	flocculants					771:781	the three magnetic carboxymethyl chitosan-based flocculants	723:781	the three magnetic carboxymethyl chitosan-based flocculants	723:781	Characterization results show that the three magnetic carboxymethyl chitosan-based flocculants have been successfully prepared with good magnetic induction properties.
34465434	5	52	theme	6	1039:1039	arg1	strength					1120:1127	precipitation magnetic field strength	1091:1127	precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively	1091:1232	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	52	theme	6	1039:1039	arg1	value					1069:1073	G value	1067:1073	G value of 200 s-1	1067:1084	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	52	theme	6	1039:1039	arg1	conditions					993:1002	the conditions	989:1002	the conditions of 80 mg/L flocculant	989:1024	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	52	theme	6	1039:1039	arg1	time					1051:1054	reaction time	1042:1054	reaction time of 1.5 hr	1042:1064	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	52	theme	6	1039:1039	arg1	value					1030:1034	pH value	1027:1034	pH value of 6	1027:1039	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	0	53	theme	chitosan-based	46:59	arg1	composite					70:78	chitosan-based magnetic composite	46:78	chitosan-based magnetic composite	46:78	Heavy metal removal from aqueous solutions by chitosan-based magnetic composite flocculants.
34465434	1	54	theme	acid	341:344	arg1	flocculant					403:412	2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant	304:412	2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA)	304:419	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	1	54	theme	acid	341:344	arg1	MCAA					415:418	MCAA	415:418	MCAA	415:418	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	5	55	theme	reaction	1042:1049	arg1	time					1051:1054	reaction time	1042:1054	reaction time of 1.5 hr	1042:1064	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	8	56	theme	flocculation	1487:1498	arg1	performance					1500:1510	excellent flocculation performance	1477:1510	excellent flocculation performance	1477:1510	The magnetic carboxymethyl chitosan-based flocculant shows excellent flocculation performance in removing soluble heavy metals.
34465434	5	57	theme	flocculant	1015:1024	arg1	strength					1120:1127	precipitation magnetic field strength	1091:1127	precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively	1091:1232	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	57	theme	flocculant	1015:1024	arg1	value					1069:1073	G value	1067:1073	G value of 200 s-1	1067:1084	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	57	theme	flocculant	1015:1024	arg1	conditions					993:1002	the conditions	989:1002	the conditions of 80 mg/L flocculant	989:1024	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	57	theme	flocculant	1015:1024	arg1	time					1051:1054	reaction time	1042:1054	reaction time of 1.5 hr	1042:1064	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	57	theme	flocculant	1015:1024	arg1	value					1030:1034	pH value	1027:1034	pH value of 6	1027:1039	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	1	58	theme	copolyacrylamide-grafted	346:369	arg1	flocculant					403:412	2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant	304:412	2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA)	304:419	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	1	58	theme	copolyacrylamide-grafted	346:369	arg1	MCAA					415:418	MCAA	415:418	MCAA	415:418	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	5	59	theme	pH	1027:1028	arg1	value					1030:1034	pH value	1027:1034	pH value of 6	1027:1039	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	1	60	theme	magnetic	114:121	arg1	flocculants					123:133	three magnetic flocculants	108:133	three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA)	108:419	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	1	61	theme	magnetic	371:378	arg1	flocculant					403:412	2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant	304:412	2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA)	304:419	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	1	61	theme	magnetic	371:378	arg1	MCAA					415:418	MCAA	415:418	MCAA	415:418	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	9	62	from	metals	1674:1679	arg1	wastewater					1684:1693	wastewater	1684:1693	wastewater	1684:1693	This research provides guidance and ideas for the development of efficient and low-cost flocculation technology to remove heavy metals in wastewater.
34465434	8	63	theme	soluble	1524:1530	arg1	metals					1538:1543	soluble heavy metals	1524:1543	soluble heavy metals	1524:1543	The magnetic carboxymethyl chitosan-based flocculant shows excellent flocculation performance in removing soluble heavy metals.
34465434	5	64	theme	removal	891:897	arg1	%					946:946	51.79%	941:946	51.79%	941:946	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	64	theme	removal	891:897	arg1	rates					899:903	the removal rates	887:903	the removal rates of MC, MCM, and MCAA on Cr(III)	887:935	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	65	theme	120 mT.	1132:1138	arg1	strength					1120:1127	precipitation magnetic field strength	1091:1127	precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively	1091:1232	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	65	theme	120 mT.	1132:1138	arg1	value					1069:1073	G value	1067:1073	G value of 200 s-1	1067:1084	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	65	theme	120 mT.	1132:1138	arg1	conditions					993:1002	the conditions	989:1002	the conditions of 80 mg/L flocculant	989:1024	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	65	theme	120 mT.	1132:1138	arg1	time					1051:1054	reaction time	1042:1054	reaction time of 1.5 hr	1042:1064	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	65	theme	120 mT.	1132:1138	arg1	value					1030:1034	pH value	1027:1034	pH value of 6	1027:1039	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	2	66	theme	Pb	572:573	arg1	removal					540:546	the flocculation removal	523:546	the flocculation removal of Cr(III), Co(II), and Pb(II)	523:577	They were applied to the flocculation removal of Cr(III), Co(II), and Pb(II).
34465434	1	67	theme	photopolymerization	470:488	arg1	technology					490:499	photopolymerization technology	470:499	photopolymerization technology	470:499	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	8	68	theme	heavy	1532:1536	arg1	metals					1538:1543	soluble heavy metals	1524:1543	soluble heavy metals	1524:1543	The magnetic carboxymethyl chitosan-based flocculant shows excellent flocculation performance in removing soluble heavy metals.
34465434	5	69	theme	Flocculation	856:867	arg1	results					869:875	Flocculation results	856:875	Flocculation results	856:875	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	9	70	theme	efficient	1611:1619	arg1	technology					1647:1656	efficient and low-cost flocculation technology	1611:1656	efficient and low-cost flocculation technology	1611:1656	This research provides guidance and ideas for the development of efficient and low-cost flocculation technology to remove heavy metals in wastewater.
34465434	5	71	theme	200 s-1	1078:1084	arg1	strength					1120:1127	precipitation magnetic field strength	1091:1127	precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively	1091:1232	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	71	theme	200 s-1	1078:1084	arg1	value					1069:1073	G value	1067:1073	G value of 200 s-1	1067:1084	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	71	theme	200 s-1	1078:1084	arg1	conditions					993:1002	the conditions	989:1002	the conditions of 80 mg/L flocculant	989:1024	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	71	theme	200 s-1	1078:1084	arg1	time					1051:1054	reaction time	1042:1054	reaction time of 1.5 hr	1042:1064	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	71	theme	200 s-1	1078:1084	arg1	value					1030:1034	pH value	1027:1034	pH value of 6	1027:1039	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	3	72	theme	flocculation	594:605	arg1	condition					607:615	flocculation condition	594:615	flocculation condition	594:615	The effect of flocculation condition on the removal performance of Cr(III), Co(II), and Pb(II) was studied.
34465434	6	73	theme	removal	1239:1245	arg1	%					1293:1293	61.54%	1288:1293	61.54%	1288:1293	The removal rates of Pb(II) by MC, MCM, and MCAA are 61.54%, 91.32%, and 95.74%, respectively.
34465434	6	73	theme	removal	1239:1245	arg1	rates					1247:1251	The removal rates	1235:1251	The removal rates of Pb(II) by MC, MCM, and MCAA	1235:1282	The removal rates of Pb(II) by MC, MCM, and MCAA are 61.54%, 91.32%, and 95.74%, respectively.
34465434	1	74	theme	different	140:148	arg1	groups					160:165	different chelating groups	140:165	different chelating groups	140:165	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	5	75	theme	removal	1144:1150	arg1	rates					1152:1156	The removal rates	1140:1156	The removal rates of Co(II) by MC, MCM, and MCAA	1140:1187	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	5	75	theme	removal	1144:1150	arg1	%					1198:1198	54.33%	1193:1198	54.33%	1193:1198	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	1	76	theme	acrylamide-grafted	231:248	arg1	MCM					294:296	MCM	294:296	MCM	294:296	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	1	76	theme	acrylamide-grafted	231:248	arg1	flocculant					282:291	acrylamide-grafted magnetic carboxymethyl chitosan flocculant	231:291	acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM)	231:297	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	0	77	theme	magnetic	61:68	arg1	composite					70:78	chitosan-based magnetic composite	46:78	chitosan-based magnetic composite	46:78	Heavy metal removal from aqueous solutions by chitosan-based magnetic composite flocculants.
34465434	1	78	theme	carboxymethyl	380:392	arg1	flocculant					403:412	2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant	304:412	2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA)	304:419	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	1	78	theme	carboxymethyl	380:392	arg1	MCAA					415:418	MCAA	415:418	MCAA	415:418	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	3	79	theme	condition	607:615	arg1	effect					584:589	The effect	580:589	The effect of flocculation condition on the removal performance of Cr(III), Co(II), and Pb(II)	580:673	The effect of flocculation condition on the removal performance of Cr(III), Co(II), and Pb(II) was studied.
34465434	1	80	theme	chelating	150:158	arg1	groups					160:165	different chelating groups	140:165	different chelating groups	140:165	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	5	81	theme	precipitation	1091:1103	arg1	strength					1120:1127	precipitation magnetic field strength	1091:1127	precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively	1091:1232	Flocculation results show that the removal rates of MC, MCM, and MCAA on Cr(III) are 51.79%, 82.33%, and 91.42%, respectively, under the conditions of 80 mg/L flocculant, pH value of 6, reaction time of 1.5 hr, G value of 200 s-1, and precipitation magnetic field strength of 120 mT. The removal rates of Co(II) by MC, MCM, and MCAA are 54.33%, 84.99%, and 90.49%, respectively.
34465434	1	82	theme	magnetic	250:257	arg1	MCM					294:296	MCM	294:296	MCM	294:296	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
34465434	1	82	theme	magnetic	250:257	arg1	flocculant					282:291	acrylamide-grafted magnetic carboxymethyl chitosan flocculant	231:291	acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM)	231:297	In this study, three magnetic flocculants with different chelating groups, namely, carboxymethyl chitosan-modified Fe3O4 flocculant (MC), acrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCM), and 2-acrylamide-2-methylpropanesulfonic acid copolyacrylamide-grafted magnetic carboxymethyl chitosan flocculant (MCAA) were prepared, synthesized, and characterized by photopolymerization technology.
33142575	2	0	used	used	334:337	arg2	polymer					273:279	a polymer	271:279	a polymer	271:279	Chitosan (CTS) is a polymer extracted from shrimp shells and crabs, which can be used to make biodegradable materials.
33142575	2	0	used	used	334:337	arg2	Chitosan					253:260	Chitosan	253:260	Chitosan (CTS)	253:266	Chitosan (CTS) is a polymer extracted from shrimp shells and crabs, which can be used to make biodegradable materials.
33142575	4	1	with	homogeneous	807:817	arg1	increase					841:848	the increase	837:848	the increase in the content of pluronic F127	837:880	The surface of starch-chitosan composite films obtained was more homogeneous and smoother with the increase in the content of pluronic F127.
33142575	2	2	theme	biodegradable	347:359	arg1	materials					361:369	biodegradable materials	347:369	biodegradable materials	347:369	Chitosan (CTS) is a polymer extracted from shrimp shells and crabs, which can be used to make biodegradable materials.
33142575	3	3	theme	copolymer	447:455	arg1	F127					466:469	the copolymer pluronic F127	443:469	the copolymer pluronic F127	443:469	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	5	4	theme	m-1s-1	983:988	arg1	Pa-1					990:993	10-14 g. m-1s-1 Pa-1	974:993	10-14 g. m-1s-1 Pa-1	974:993	The %S and WVP of the starch-chitosan films decreased from 42 to 3%, and 21 × 10-11 to 3 × 10-14 g. m-1s-1 Pa-1, respectively, with the incorporation of pluronic from 3%, which makes these materials a good alternative for product packaging.
33142575	5	5	dep	×	959:959	arg1	to					967:968	to	967:968	to	967:968	The %S and WVP of the starch-chitosan films decreased from 42 to 3%, and 21 × 10-11 to 3 × 10-14 g. m-1s-1 Pa-1, respectively, with the incorporation of pluronic from 3%, which makes these materials a good alternative for product packaging.
33142575	3	6	theme	several	491:497	arg1	concentrations					499:512	several concentrations	491:512	several concentrations	491:512	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	1	7	theme	other	237:241	arg1	polymers					243:250	other polymers	237:250	other polymers	237:250	Starch is an excellent alternative to produce packaging materials, however, due to its high hydrophilicity, it is necessary to mix it with other polymers.
33142575	3	8	theme	corn	417:420	arg1	starch					422:427	corn starch	417:427	corn starch	417:427	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	3	8	theme	corn	417:420	arg1	component					386:394	The principal component	372:394	The principal component of biodegradable	372:411	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	5	9	theme	starch-chitosan	905:919	arg1	films					921:925	the starch-chitosan films	901:925	the starch-chitosan films	901:925	The %S and WVP of the starch-chitosan films decreased from 42 to 3%, and 21 × 10-11 to 3 × 10-14 g. m-1s-1 Pa-1, respectively, with the incorporation of pluronic from 3%, which makes these materials a good alternative for product packaging.
33142575	3	10	theme	principal	376:384	arg1	starch					422:427	corn starch	417:427	corn starch	417:427	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	3	10	theme	principal	376:384	arg1	component					386:394	The principal component	372:394	The principal component of biodegradable	372:411	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	5	11	theme	good	1084:1087	arg1	alternative					1089:1099	a good alternative	1082:1099	a good alternative for product packaging	1082:1121	The %S and WVP of the starch-chitosan films decreased from 42 to 3%, and 21 × 10-11 to 3 × 10-14 g. m-1s-1 Pa-1, respectively, with the incorporation of pluronic from 3%, which makes these materials a good alternative for product packaging.
33142575	5	12	theme	films	921:925	arg1	WVP					894:896	WVP	894:896	WVP	894:896	The %S and WVP of the starch-chitosan films decreased from 42 to 3%, and 21 × 10-11 to 3 × 10-14 g. m-1s-1 Pa-1, respectively, with the incorporation of pluronic from 3%, which makes these materials a good alternative for product packaging.
33142575	5	12	theme	films	921:925	arg1	S					888:888	%S	887:888	%S	887:888	The %S and WVP of the starch-chitosan films decreased from 42 to 3%, and 21 × 10-11 to 3 × 10-14 g. m-1s-1 Pa-1, respectively, with the incorporation of pluronic from 3%, which makes these materials a good alternative for product packaging.
33142575	5	13	from	%	1051:1051	arg1	incorporation					1019:1031	the incorporation	1015:1031	the incorporation of pluronic from 3%, which makes these materials a good alternative for product packaging	1015:1121	The %S and WVP of the starch-chitosan films decreased from 42 to 3%, and 21 × 10-11 to 3 × 10-14 g. m-1s-1 Pa-1, respectively, with the incorporation of pluronic from 3%, which makes these materials a good alternative for product packaging.
33142575	5	14	theme	%	887:887	arg1	S					888:888	%S	887:888	%S	887:888	The %S and WVP of the starch-chitosan films decreased from 42 to 3%, and 21 × 10-11 to 3 × 10-14 g. m-1s-1 Pa-1, respectively, with the incorporation of pluronic from 3%, which makes these materials a good alternative for product packaging.
33142575	5	15	dep	3	948:948	arg1	to					945:946	to	945:946	to	945:946	The %S and WVP of the starch-chitosan films decreased from 42 to 3%, and 21 × 10-11 to 3 × 10-14 g. m-1s-1 Pa-1, respectively, with the incorporation of pluronic from 3%, which makes these materials a good alternative for product packaging.
33142575	3	16	from	incorporation	647:659	arg1	formulation					668:678	the formulation	664:678	the formulation of biodegradable materials	664:705	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	3	17	theme	biodegradable	683:695	arg1	materials					697:705	biodegradable materials	683:705	biodegradable materials	683:705	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	0	18	theme	novel	17:21	arg1	film					47:50	a novel biodegradable packaging film	15:50	a novel biodegradable packaging film	15:50	Preparation of a novel biodegradable packaging film based on corn starch-chitosan and poloxamers.
33142575	3	19	theme	materials	697:705	arg1	formulation					668:678	the formulation	664:678	the formulation of biodegradable materials	664:705	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	4	20	theme	F127	877:880	arg1	content					857:863	the content	853:863	the content of pluronic F127	853:880	The surface of starch-chitosan composite films obtained was more homogeneous and smoother with the increase in the content of pluronic F127.
33142575	4	21	theme	composite	773:781	arg1	films					783:787	starch-chitosan composite films	757:787	starch-chitosan composite films obtained	757:796	The surface of starch-chitosan composite films obtained was more homogeneous and smoother with the increase in the content of pluronic F127.
33142575	4	22	theme	pluronic	868:875	arg1	F127					877:880	pluronic F127	868:880	pluronic F127	868:880	The surface of starch-chitosan composite films obtained was more homogeneous and smoother with the increase in the content of pluronic F127.
33142575	5	23	dep	S	888:888	arg1	The					883:885	The	883:885	The	883:885	The %S and WVP of the starch-chitosan films decreased from 42 to 3%, and 21 × 10-11 to 3 × 10-14 g. m-1s-1 Pa-1, respectively, with the incorporation of pluronic from 3%, which makes these materials a good alternative for product packaging.
33142575	1	24	theme	high	185:188	arg1	hydrophilicity					190:203	its high hydrophilicity	181:203	its high hydrophilicity	181:203	Starch is an excellent alternative to produce packaging materials, however, due to its high hydrophilicity, it is necessary to mix it with other polymers.
33142575	0	25	theme	packaging	37:45	arg1	film					47:50	a novel biodegradable packaging film	15:50	a novel biodegradable packaging film	15:50	Preparation of a novel biodegradable packaging film based on corn starch-chitosan and poloxamers.
33142575	3	26	theme	mechanical	585:594	arg1	barrier					548:554	the water vapor barrier	532:554	the water vapor barrier	532:554	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	3	26	theme	mechanical	585:594	arg1	properties					596:605	morphological, thermal, and mechanical properties	557:605	morphological, thermal, and mechanical properties of the films	557:618	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	3	27	theme	water	536:540	arg1	properties					596:605	morphological, thermal, and mechanical properties	557:605	morphological, thermal, and mechanical properties of the films	557:618	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	3	27	theme	water	536:540	arg1	barrier					548:554	the water vapor barrier	532:554	the water vapor barrier	532:554	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	5	28	with	Pa-1	990:993	arg1	incorporation					1019:1031	the incorporation	1015:1031	the incorporation of pluronic from 3%, which makes these materials a good alternative for product packaging	1015:1121	The %S and WVP of the starch-chitosan films decreased from 42 to 3%, and 21 × 10-11 to 3 × 10-14 g. m-1s-1 Pa-1, respectively, with the incorporation of pluronic from 3%, which makes these materials a good alternative for product packaging.
33142575	0	29	theme	biodegradable	23:35	arg1	film					47:50	a novel biodegradable packaging film	15:50	a novel biodegradable packaging film	15:50	Preparation of a novel biodegradable packaging film based on corn starch-chitosan and poloxamers.
33142575	3	30	theme	thermal	572:578	arg1	barrier					548:554	the water vapor barrier	532:554	the water vapor barrier	532:554	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	3	30	theme	thermal	572:578	arg1	properties					596:605	morphological, thermal, and mechanical properties	557:605	morphological, thermal, and mechanical properties of the films	557:618	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	3	31	theme	vapor	542:546	arg1	properties					596:605	morphological, thermal, and mechanical properties	557:605	morphological, thermal, and mechanical properties of the films	557:618	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	3	31	theme	vapor	542:546	arg1	barrier					548:554	the water vapor barrier	532:554	the water vapor barrier	532:554	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	3	32	theme	biodegradable	399:411	arg1	starch					422:427	corn starch	417:427	corn starch	417:427	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	3	32	theme	biodegradable	399:411	arg1	component					386:394	The principal component	372:394	The principal component of biodegradable	372:411	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	5	33	theme	pluronic	1036:1043	arg1	incorporation					1019:1031	the incorporation	1015:1031	the incorporation of pluronic from 3%, which makes these materials a good alternative for product packaging	1015:1121	The %S and WVP of the starch-chitosan films decreased from 42 to 3%, and 21 × 10-11 to 3 × 10-14 g. m-1s-1 Pa-1, respectively, with the incorporation of pluronic from 3%, which makes these materials a good alternative for product packaging.
33142575	5	34	theme	g.	980:981	arg1	Pa-1					990:993	10-14 g. m-1s-1 Pa-1	974:993	10-14 g. m-1s-1 Pa-1	974:993	The %S and WVP of the starch-chitosan films decreased from 42 to 3%, and 21 × 10-11 to 3 × 10-14 g. m-1s-1 Pa-1, respectively, with the incorporation of pluronic from 3%, which makes these materials a good alternative for product packaging.
33142575	2	35	theme	shrimp	296:301	arg1	shells					303:308	shrimp shells	296:308	shrimp shells	296:308	Chitosan (CTS) is a polymer extracted from shrimp shells and crabs, which can be used to make biodegradable materials.
33142575	0	36	theme	film	47:50	arg1	Preparation					0:10	Preparation	0:10	Preparation of a novel biodegradable packaging film	0:50	Preparation of a novel biodegradable packaging film based on corn starch-chitosan and poloxamers.
33142575	4	37	theme	films	783:787	arg1	surface					746:752	The surface	742:752	The surface of starch-chitosan composite films obtained	742:796	The surface of starch-chitosan composite films obtained was more homogeneous and smoother with the increase in the content of pluronic F127.
33142575	4	37	theme	films	783:787	arg1	homogeneous					807:817	homogeneous	807:817	homogeneous	807:817	The surface of starch-chitosan composite films obtained was more homogeneous and smoother with the increase in the content of pluronic F127.
33142575	1	38	with	mix	225:227	arg1	polymers					243:250	other polymers	237:250	other polymers	237:250	Starch is an excellent alternative to produce packaging materials, however, due to its high hydrophilicity, it is necessary to mix it with other polymers.
33142575	1	39	theme	excellent	111:119	arg1	alternative					121:131	an excellent alternative	108:131	an excellent alternative to produce packaging materials	108:162	Starch is an excellent alternative to produce packaging materials, however, due to its high hydrophilicity, it is necessary to mix it with other polymers.
33142575	1	39	theme	excellent	111:119	arg1	Starch					98:103	Starch	98:103	Starch	98:103	Starch is an excellent alternative to produce packaging materials, however, due to its high hydrophilicity, it is necessary to mix it with other polymers.
33142575	3	40	theme	films	614:618	arg1	barrier					548:554	the water vapor barrier	532:554	the water vapor barrier	532:554	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	3	40	theme	films	614:618	arg1	properties					596:605	morphological, thermal, and mechanical properties	557:605	morphological, thermal, and mechanical properties of the films	557:618	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	3	41	theme	morphological	557:569	arg1	barrier					548:554	the water vapor barrier	532:554	the water vapor barrier	532:554	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	3	41	theme	morphological	557:569	arg1	properties					596:605	morphological, thermal, and mechanical properties	557:605	morphological, thermal, and mechanical properties of the films	557:618	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	4	42	theme	starch-chitosan	757:771	arg1	films					783:787	starch-chitosan composite films	757:787	starch-chitosan composite films obtained	757:796	The surface of starch-chitosan composite films obtained was more homogeneous and smoother with the increase in the content of pluronic F127.
33142575	0	43	theme	corn	61:64	arg1	starch-chitosan					66:80	corn starch-chitosan	61:80	corn starch-chitosan	61:80	Preparation of a novel biodegradable packaging film based on corn starch-chitosan and poloxamers.
33142575	3	44	theme	pluronic	457:464	arg1	F127					466:469	the copolymer pluronic F127	443:469	the copolymer pluronic F127	443:469	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	3	45	from	effect	522:527	arg1	properties					596:605	morphological, thermal, and mechanical properties	557:605	morphological, thermal, and mechanical properties of the films	557:618	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	3	45	from	effect	522:527	arg1	barrier					548:554	the water vapor barrier	532:554	the water vapor barrier	532:554	The principal component of biodegradable was corn starch and chitosan, the copolymer pluronic F127 was incorporated in several concentrations and its effect on the water vapor barrier, morphological, thermal, and mechanical properties of the films was evaluated, because its incorporation in the formulation of biodegradable materials could increase its hydrophobicity.
33142575	5	46	theme	product	1105:1111	arg1	packaging					1113:1121	product packaging	1105:1121	product packaging	1105:1121	The %S and WVP of the starch-chitosan films decreased from 42 to 3%, and 21 × 10-11 to 3 × 10-14 g. m-1s-1 Pa-1, respectively, with the incorporation of pluronic from 3%, which makes these materials a good alternative for product packaging.
33142575	4	47	from	increase	841:848	arg1	content					857:863	the content	853:863	the content of pluronic F127	853:880	The surface of starch-chitosan composite films obtained was more homogeneous and smoother with the increase in the content of pluronic F127.
33142575	1	48	theme	packaging	144:152	arg1	materials					154:162	packaging materials	144:162	packaging materials	144:162	Starch is an excellent alternative to produce packaging materials, however, due to its high hydrophilicity, it is necessary to mix it with other polymers.
33240285	0	0	theme	Gut	92:94	arg1	Microbiota					96:105	Gut Microbiota	92:105	Gut Microbiota	92:105	Effects of Polysaccharides From Auricularia auricula on the Immuno-Stimulatory Activity and Gut Microbiota in Immunosuppressed Mice Induced by Cyclophosphamide.
33240285	7	1	theme	microbiota	1023:1032	arg1	composition					1034:1044	the gut microbiota composition	1015:1044	the gut microbiota composition similar to that of the control group	1015:1081	Compared to the CTX group, AAP1 administration restored the gut microbiota composition similar to that of the control group by decreasing the ratio of Firmicutes/Bacteroidetes and increasing the relative abundances of short-chain fatty acid-producing microbiota.
33240285	6	2	theme	mRNA	914:917	arg1	levels					919:924	the mRNA levels	910:924	the mRNA levels of Claudin-1, Occludin and ZO-1	910:956	Results showed that AAP1 significantly enhanced immune organ indexes, remarkably stimulated IFN-γ, IL-2, IL-4, IL-10, and TNF-α levels in the serum, and dramatically up-regulated the mRNA levels of Claudin-1, Occludin and ZO-1.
33240285	7	3	theme	control	1069:1075	arg1	group					1077:1081	the control group	1065:1081	the control group	1065:1081	Compared to the CTX group, AAP1 administration restored the gut microbiota composition similar to that of the control group by decreasing the ratio of Firmicutes/Bacteroidetes and increasing the relative abundances of short-chain fatty acid-producing microbiota.
33240285	1	4	theme	immuno-enhancing	175:190	arg1	potential					192:200	the immuno-enhancing potential	171:200	the immuno-enhancing potential of polysaccharide from Auricularia auricula (AAP)	171:250	Recently, the immuno-enhancing potential of polysaccharide from Auricularia auricula (AAP) has been an area of research interest.
33240285	1	4	theme	immuno-enhancing	175:190	arg1	area					264:267	an area	261:267	an area of research interest	261:288	Recently, the immuno-enhancing potential of polysaccharide from Auricularia auricula (AAP) has been an area of research interest.
33240285	2	5	from	mechanisms	336:345	arg1	mice					375:378	immunosuppressive mice	357:378	immunosuppressive mice treated with cyclophosphamide (CTX)	357:414	However, the immune-stimulatory activity and mechanisms of AAP in immunosuppressive mice treated with cyclophosphamide (CTX) are still poorly understood.
33240285	0	6	from	Effects	0:6	arg1	auricula					44:51	Auricularia auricula	32:51	Auricularia auricula	32:51	Effects of Polysaccharides From Auricularia auricula on the Immuno-Stimulatory Activity and Gut Microbiota in Immunosuppressed Mice Induced by Cyclophosphamide.
33240285	0	6	from	Effects	0:6	arg1	Activity					79:86	Immuno-Stimulatory Activity	60:86	Immuno-Stimulatory Activity	60:86	Effects of Polysaccharides From Auricularia auricula on the Immuno-Stimulatory Activity and Gut Microbiota in Immunosuppressed Mice Induced by Cyclophosphamide.
33240285	0	6	from	Effects	0:6	arg1	Microbiota					96:105	Gut Microbiota	92:105	Gut Microbiota	92:105	Effects of Polysaccharides From Auricularia auricula on the Immuno-Stimulatory Activity and Gut Microbiota in Immunosuppressed Mice Induced by Cyclophosphamide.
33240285	5	7	theme	immune-stimulatory	628:645	arg1	activities					647:656	the immune-stimulatory activities	624:656	the immune-stimulatory activities of the first AAP fraction (AAP1)	624:689	Secondly, the immune-stimulatory activities of the first AAP fraction (AAP1) were evaluated in the CTX-treated mice.
33240285	4	8	attach	isolated	574:581	arg1	A. auricula					588:598	A. auricula	588:598	A. auricula	588:598	Firstly, polysaccharides were isolated from A. auricula and purified.
33240285	4	8	attach	isolated	574:581	arg2	polysaccharides					553:567	polysaccharides	553:567	polysaccharides	553:567	Firstly, polysaccharides were isolated from A. auricula and purified.
33240285	7	9	theme	CTX	975:977	arg1	group					979:983	the CTX group	971:983	the CTX group	971:983	Compared to the CTX group, AAP1 administration restored the gut microbiota composition similar to that of the control group by decreasing the ratio of Firmicutes/Bacteroidetes and increasing the relative abundances of short-chain fatty acid-producing microbiota.
33240285	1	10	theme	research	272:279	arg1	interest					281:288	research interest	272:288	research interest	272:288	Recently, the immuno-enhancing potential of polysaccharide from Auricularia auricula (AAP) has been an area of research interest.
33240285	3	11	dep	AAP	506:508	arg1	mechanisms					532:541	its possible mechanisms	519:541	its possible mechanisms	519:541	This study aimed to evaluate the immuno-enhancing effects of AAP and mine its possible mechanisms.
33240285	1	12	theme	interest	281:288	arg1	potential					192:200	the immuno-enhancing potential	171:200	the immuno-enhancing potential of polysaccharide from Auricularia auricula (AAP)	171:250	Recently, the immuno-enhancing potential of polysaccharide from Auricularia auricula (AAP) has been an area of research interest.
33240285	1	12	theme	interest	281:288	arg1	area					264:267	an area	261:267	an area of research interest	261:288	Recently, the immuno-enhancing potential of polysaccharide from Auricularia auricula (AAP) has been an area of research interest.
33240285	7	13	theme	acid-producing	1195:1208	arg1	microbiota					1210:1219	short-chain fatty acid-producing microbiota	1177:1219	short-chain fatty acid-producing microbiota	1177:1219	Compared to the CTX group, AAP1 administration restored the gut microbiota composition similar to that of the control group by decreasing the ratio of Firmicutes/Bacteroidetes and increasing the relative abundances of short-chain fatty acid-producing microbiota.
33240285	2	14	dep	activity	323:330	arg1	the					300:302	the	300:302	the	300:302	However, the immune-stimulatory activity and mechanisms of AAP in immunosuppressive mice treated with cyclophosphamide (CTX) are still poorly understood.
33240285	6	15	theme	immune	779:784	arg1	indexes					792:798	immune organ indexes	779:798	immune organ indexes	779:798	Results showed that AAP1 significantly enhanced immune organ indexes, remarkably stimulated IFN-γ, IL-2, IL-4, IL-10, and TNF-α levels in the serum, and dramatically up-regulated the mRNA levels of Claudin-1, Occludin and ZO-1.
33240285	0	16	theme	Immunosuppressed	110:125	arg1	Mice					127:130	Immunosuppressed Mice	110:130	Immunosuppressed Mice	110:130	Effects of Polysaccharides From Auricularia auricula on the Immuno-Stimulatory Activity and Gut Microbiota in Immunosuppressed Mice Induced by Cyclophosphamide.
33240285	0	17	from	auricula	44:51	arg1	Polysaccharides					11:25	Polysaccharides	11:25	Polysaccharides From Auricularia auricula	11:51	Effects of Polysaccharides From Auricularia auricula on the Immuno-Stimulatory Activity and Gut Microbiota in Immunosuppressed Mice Induced by Cyclophosphamide.
33240285	0	17	from	auricula	44:51	arg1	Effects					0:6	Effects	0:6	Effects of Polysaccharides From Auricularia auricula on the Immuno-Stimulatory Activity and Gut Microbiota in Immunosuppressed Mice	0:130	Effects of Polysaccharides From Auricularia auricula on the Immuno-Stimulatory Activity and Gut Microbiota in Immunosuppressed Mice Induced by Cyclophosphamide.
33240285	5	18	theme	first	665:669	arg1	AAP1					685:688	AAP1	685:688	AAP1	685:688	Secondly, the immune-stimulatory activities of the first AAP fraction (AAP1) were evaluated in the CTX-treated mice.
33240285	5	18	theme	first	665:669	arg1	fraction					675:682	the first AAP fraction	661:682	the first AAP fraction (AAP1)	661:689	Secondly, the immune-stimulatory activities of the first AAP fraction (AAP1) were evaluated in the CTX-treated mice.
33240285	7	19	theme	fatty	1189:1193	arg1	microbiota					1210:1219	short-chain fatty acid-producing microbiota	1177:1219	short-chain fatty acid-producing microbiota	1177:1219	Compared to the CTX group, AAP1 administration restored the gut microbiota composition similar to that of the control group by decreasing the ratio of Firmicutes/Bacteroidetes and increasing the relative abundances of short-chain fatty acid-producing microbiota.
33240285	6	20	from	IL-2	830:833	arg1	serum					873:877	the serum	869:877	the serum	869:877	Results showed that AAP1 significantly enhanced immune organ indexes, remarkably stimulated IFN-γ, IL-2, IL-4, IL-10, and TNF-α levels in the serum, and dramatically up-regulated the mRNA levels of Claudin-1, Occludin and ZO-1.
33240285	7	21	theme	short-chain	1177:1187	arg1	microbiota					1210:1219	short-chain fatty acid-producing microbiota	1177:1219	short-chain fatty acid-producing microbiota	1177:1219	Compared to the CTX group, AAP1 administration restored the gut microbiota composition similar to that of the control group by decreasing the ratio of Firmicutes/Bacteroidetes and increasing the relative abundances of short-chain fatty acid-producing microbiota.
33240285	5	22	theme	AAP	671:673	arg1	AAP1					685:688	AAP1	685:688	AAP1	685:688	Secondly, the immune-stimulatory activities of the first AAP fraction (AAP1) were evaluated in the CTX-treated mice.
33240285	5	22	theme	AAP	671:673	arg1	fraction					675:682	the first AAP fraction	661:682	the first AAP fraction (AAP1)	661:689	Secondly, the immune-stimulatory activities of the first AAP fraction (AAP1) were evaluated in the CTX-treated mice.
33240285	1	23	theme	polysaccharide	205:218	arg1	potential					192:200	the immuno-enhancing potential	171:200	the immuno-enhancing potential of polysaccharide from Auricularia auricula (AAP)	171:250	Recently, the immuno-enhancing potential of polysaccharide from Auricularia auricula (AAP) has been an area of research interest.
33240285	1	23	theme	polysaccharide	205:218	arg1	area					264:267	an area	261:267	an area of research interest	261:288	Recently, the immuno-enhancing potential of polysaccharide from Auricularia auricula (AAP) has been an area of research interest.
33240285	6	24	from	levels	859:864	arg1	serum					873:877	the serum	869:877	the serum	869:877	Results showed that AAP1 significantly enhanced immune organ indexes, remarkably stimulated IFN-γ, IL-2, IL-4, IL-10, and TNF-α levels in the serum, and dramatically up-regulated the mRNA levels of Claudin-1, Occludin and ZO-1.
33240285	5	25	theme	fraction	675:682	arg1	activities					647:656	the immune-stimulatory activities	624:656	the immune-stimulatory activities of the first AAP fraction (AAP1)	624:689	Secondly, the immune-stimulatory activities of the first AAP fraction (AAP1) were evaluated in the CTX-treated mice.
33240285	6	26	from	IL-4	836:839	arg1	serum					873:877	the serum	869:877	the serum	869:877	Results showed that AAP1 significantly enhanced immune organ indexes, remarkably stimulated IFN-γ, IL-2, IL-4, IL-10, and TNF-α levels in the serum, and dramatically up-regulated the mRNA levels of Claudin-1, Occludin and ZO-1.
33240285	0	27	dep	Activity	79:86	arg1	the					56:58	the	56:58	the	56:58	Effects of Polysaccharides From Auricularia auricula on the Immuno-Stimulatory Activity and Gut Microbiota in Immunosuppressed Mice Induced by Cyclophosphamide.
33240285	3	28	theme	immuno-enhancing	478:493	arg1	effects					495:501	the immuno-enhancing effects	474:501	the immuno-enhancing effects of AAP and mine its possible mechanisms	474:541	This study aimed to evaluate the immuno-enhancing effects of AAP and mine its possible mechanisms.
33240285	0	29	theme	Polysaccharides	11:25	arg1	Effects					0:6	Effects	0:6	Effects of Polysaccharides From Auricularia auricula on the Immuno-Stimulatory Activity and Gut Microbiota in Immunosuppressed Mice	0:130	Effects of Polysaccharides From Auricularia auricula on the Immuno-Stimulatory Activity and Gut Microbiota in Immunosuppressed Mice Induced by Cyclophosphamide.
33240285	6	30	theme	organ	786:790	arg1	indexes					792:798	immune organ indexes	779:798	immune organ indexes	779:798	Results showed that AAP1 significantly enhanced immune organ indexes, remarkably stimulated IFN-γ, IL-2, IL-4, IL-10, and TNF-α levels in the serum, and dramatically up-regulated the mRNA levels of Claudin-1, Occludin and ZO-1.
33240285	2	31	theme	immune-stimulatory	304:321	arg1	activity					323:330	immune-stimulatory activity	304:330	immune-stimulatory activity	304:330	However, the immune-stimulatory activity and mechanisms of AAP in immunosuppressive mice treated with cyclophosphamide (CTX) are still poorly understood.
33240285	6	32	theme	ZO-1	953:956	arg1	levels					919:924	the mRNA levels	910:924	the mRNA levels of Claudin-1, Occludin and ZO-1	910:956	Results showed that AAP1 significantly enhanced immune organ indexes, remarkably stimulated IFN-γ, IL-2, IL-4, IL-10, and TNF-α levels in the serum, and dramatically up-regulated the mRNA levels of Claudin-1, Occludin and ZO-1.
33240285	8	33	theme	further	1269:1275	arg1	application					1277:1287	its further application	1265:1287	its further application as an immune-stimulator in foods and drugs	1265:1330	This study provides useful information for its further application as an immune-stimulator in foods and drugs.
33240285	6	34	from	IL-10	842:846	arg1	serum					873:877	the serum	869:877	the serum	869:877	Results showed that AAP1 significantly enhanced immune organ indexes, remarkably stimulated IFN-γ, IL-2, IL-4, IL-10, and TNF-α levels in the serum, and dramatically up-regulated the mRNA levels of Claudin-1, Occludin and ZO-1.
33240285	7	35	theme	relative	1154:1161	arg1	abundances					1163:1172	the relative abundances	1150:1172	the relative abundances of short-chain fatty acid-producing microbiota	1150:1219	Compared to the CTX group, AAP1 administration restored the gut microbiota composition similar to that of the control group by decreasing the ratio of Firmicutes/Bacteroidetes and increasing the relative abundances of short-chain fatty acid-producing microbiota.
33240285	0	36	from	Activity	79:86	arg1	Mice					127:130	Immunosuppressed Mice	110:130	Immunosuppressed Mice	110:130	Effects of Polysaccharides From Auricularia auricula on the Immuno-Stimulatory Activity and Gut Microbiota in Immunosuppressed Mice Induced by Cyclophosphamide.
33240285	7	37	theme	microbiota	1210:1219	arg1	abundances					1163:1172	the relative abundances	1150:1172	the relative abundances of short-chain fatty acid-producing microbiota	1150:1219	Compared to the CTX group, AAP1 administration restored the gut microbiota composition similar to that of the control group by decreasing the ratio of Firmicutes/Bacteroidetes and increasing the relative abundances of short-chain fatty acid-producing microbiota.
33240285	7	38	theme	Firmicutes/Bacteroidetes	1110:1133	arg1	ratio					1101:1105	the ratio	1097:1105	the ratio of Firmicutes/Bacteroidetes	1097:1133	Compared to the CTX group, AAP1 administration restored the gut microbiota composition similar to that of the control group by decreasing the ratio of Firmicutes/Bacteroidetes and increasing the relative abundances of short-chain fatty acid-producing microbiota.
33240285	6	39	theme	Occludin	940:947	arg1	levels					919:924	the mRNA levels	910:924	the mRNA levels of Claudin-1, Occludin and ZO-1	910:956	Results showed that AAP1 significantly enhanced immune organ indexes, remarkably stimulated IFN-γ, IL-2, IL-4, IL-10, and TNF-α levels in the serum, and dramatically up-regulated the mRNA levels of Claudin-1, Occludin and ZO-1.
33240285	7	40	theme	AAP1	986:989	arg1	administration					991:1004	AAP1 administration	986:1004	AAP1 administration	986:1004	Compared to the CTX group, AAP1 administration restored the gut microbiota composition similar to that of the control group by decreasing the ratio of Firmicutes/Bacteroidetes and increasing the relative abundances of short-chain fatty acid-producing microbiota.
33240285	2	41	theme	immunosuppressive	357:373	arg1	mice					375:378	immunosuppressive mice	357:378	immunosuppressive mice treated with cyclophosphamide (CTX)	357:414	However, the immune-stimulatory activity and mechanisms of AAP in immunosuppressive mice treated with cyclophosphamide (CTX) are still poorly understood.
33240285	8	42	theme	useful	1242:1247	arg1	information					1249:1259	useful information	1242:1259	useful information for its further application as an immune-stimulator in foods and drugs	1242:1330	This study provides useful information for its further application as an immune-stimulator in foods and drugs.
33240285	7	43	theme	similar	1046:1052	arg1	composition					1034:1044	the gut microbiota composition	1015:1044	the gut microbiota composition similar to that of the control group	1015:1081	Compared to the CTX group, AAP1 administration restored the gut microbiota composition similar to that of the control group by decreasing the ratio of Firmicutes/Bacteroidetes and increasing the relative abundances of short-chain fatty acid-producing microbiota.
33240285	6	44	theme	TNF-α	853:857	arg1	IFN-γ					823:827	IFN-γ	823:827	IFN-γ	823:827	Results showed that AAP1 significantly enhanced immune organ indexes, remarkably stimulated IFN-γ, IL-2, IL-4, IL-10, and TNF-α levels in the serum, and dramatically up-regulated the mRNA levels of Claudin-1, Occludin and ZO-1.
33240285	6	44	theme	TNF-α	853:857	arg1	levels					859:864	TNF-α levels	853:864	TNF-α levels in the serum	853:877	Results showed that AAP1 significantly enhanced immune organ indexes, remarkably stimulated IFN-γ, IL-2, IL-4, IL-10, and TNF-α levels in the serum, and dramatically up-regulated the mRNA levels of Claudin-1, Occludin and ZO-1.
33240285	3	45	theme	possible	523:530	arg1	mechanisms					532:541	its possible mechanisms	519:541	its possible mechanisms	519:541	This study aimed to evaluate the immuno-enhancing effects of AAP and mine its possible mechanisms.
33240285	3	46	theme	mine	514:517	arg1	effects					495:501	the immuno-enhancing effects	474:501	the immuno-enhancing effects of AAP and mine its possible mechanisms	474:541	This study aimed to evaluate the immuno-enhancing effects of AAP and mine its possible mechanisms.
33240285	6	47	theme	Claudin-1	929:937	arg1	levels					919:924	the mRNA levels	910:924	the mRNA levels of Claudin-1, Occludin and ZO-1	910:956	Results showed that AAP1 significantly enhanced immune organ indexes, remarkably stimulated IFN-γ, IL-2, IL-4, IL-10, and TNF-α levels in the serum, and dramatically up-regulated the mRNA levels of Claudin-1, Occludin and ZO-1.
33240285	7	48	theme	gut	1019:1021	arg1	composition					1034:1044	the gut microbiota composition	1015:1044	the gut microbiota composition similar to that of the control group	1015:1081	Compared to the CTX group, AAP1 administration restored the gut microbiota composition similar to that of the control group by decreasing the ratio of Firmicutes/Bacteroidetes and increasing the relative abundances of short-chain fatty acid-producing microbiota.
33240285	1	49	from	auricula	237:244	arg1	potential					192:200	the immuno-enhancing potential	171:200	the immuno-enhancing potential of polysaccharide from Auricularia auricula (AAP)	171:250	Recently, the immuno-enhancing potential of polysaccharide from Auricularia auricula (AAP) has been an area of research interest.
33240285	1	49	from	auricula	237:244	arg1	area					264:267	an area	261:267	an area of research interest	261:288	Recently, the immuno-enhancing potential of polysaccharide from Auricularia auricula (AAP) has been an area of research interest.
33240285	2	50	theme	AAP	350:352	arg1	activity					323:330	immune-stimulatory activity	304:330	immune-stimulatory activity	304:330	However, the immune-stimulatory activity and mechanisms of AAP in immunosuppressive mice treated with cyclophosphamide (CTX) are still poorly understood.
33240285	2	50	theme	AAP	350:352	arg1	mechanisms					336:345	mechanisms	336:345	mechanisms	336:345	However, the immune-stimulatory activity and mechanisms of AAP in immunosuppressive mice treated with cyclophosphamide (CTX) are still poorly understood.
33240285	5	51	theme	CTX-treated	713:723	arg1	mice					725:728	the CTX-treated mice	709:728	the CTX-treated mice	709:728	Secondly, the immune-stimulatory activities of the first AAP fraction (AAP1) were evaluated in the CTX-treated mice.
33240285	3	52	theme	AAP	506:508	arg1	effects					495:501	the immuno-enhancing effects	474:501	the immuno-enhancing effects of AAP and mine its possible mechanisms	474:541	This study aimed to evaluate the immuno-enhancing effects of AAP and mine its possible mechanisms.
33240285	0	53	from	Microbiota	96:105	arg1	Mice					127:130	Immunosuppressed Mice	110:130	Immunosuppressed Mice	110:130	Effects of Polysaccharides From Auricularia auricula on the Immuno-Stimulatory Activity and Gut Microbiota in Immunosuppressed Mice Induced by Cyclophosphamide.
33240285	8	54	from	immune-stimulator	1295:1311	arg1	drugs					1326:1330	drugs	1326:1330	drugs	1326:1330	This study provides useful information for its further application as an immune-stimulator in foods and drugs.
33240285	8	54	from	immune-stimulator	1295:1311	arg1	foods					1316:1320	foods	1316:1320	foods	1316:1320	This study provides useful information for its further application as an immune-stimulator in foods and drugs.
33240285	0	55	theme	Immuno-Stimulatory	60:77	arg1	Activity					79:86	Immuno-Stimulatory Activity	60:86	Immuno-Stimulatory Activity	60:86	Effects of Polysaccharides From Auricularia auricula on the Immuno-Stimulatory Activity and Gut Microbiota in Immunosuppressed Mice Induced by Cyclophosphamide.
33240285	2	56	from	activity	323:330	arg1	mice					375:378	immunosuppressive mice	357:378	immunosuppressive mice treated with cyclophosphamide (CTX)	357:414	However, the immune-stimulatory activity and mechanisms of AAP in immunosuppressive mice treated with cyclophosphamide (CTX) are still poorly understood.
33955159	6	0	theme	superficial	1297:1307	arg1	velocity					1309:1316	superficial velocity	1297:1316	superficial velocity of 400 μm/s	1297:1328	The continuous SBF flow (superficial velocity of 400 μm/s) induced the formation of abundant HAP crystals throughout the scaffolds leading to improved mechanical properties to some extent as compared to the initial scaffolds.
33955159	6	1	theme	initial	1479:1485	arg1	scaffolds					1487:1495	the initial scaffolds	1475:1495	the initial scaffolds	1475:1495	The continuous SBF flow (superficial velocity of 400 μm/s) induced the formation of abundant HAP crystals throughout the scaffolds leading to improved mechanical properties to some extent as compared to the initial scaffolds.
33955159	1	2	theme	alginate	243:250	arg1	scaffolds					270:278	alginate based macroporous scaffolds	243:278	alginate based macroporous scaffolds with the aim to imitate the natural bone structure	243:329	In the present study, we synthesized hydroxyapatite (HAP) powders followed by the production of alginate based macroporous scaffolds with the aim to imitate the natural bone structure.
33955159	2	3	dep	HAP	481:483	arg1	phosphate					502:510	phosphate	502:510	phosphate	502:510	HAP powders were synthesized by using a hydrothermal method, and after calcination, dominant phases in the powders, undoped and doped with Mg2+ were HAP and β-tricalcium phosphate, respectively.
33955159	5	4	theme	[Ca + Mg	1148:1155	arg1	ratio					1166:1170	[Ca + Mg]/P molar ratio	1148:1170	[Ca + Mg]/P molar ratio of 1.52	1148:1178	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
33955159	2	5	dep	powders	439:445	arg1	doped					460:464	doped	460:464	doped with Mg2+	460:474	HAP powders were synthesized by using a hydrothermal method, and after calcination, dominant phases in the powders, undoped and doped with Mg2+ were HAP and β-tricalcium phosphate, respectively.
33955159	2	5	dep	powders	439:445	arg1	undoped					448:454	undoped	448:454	undoped	448:454	HAP powders were synthesized by using a hydrothermal method, and after calcination, dominant phases in the powders, undoped and doped with Mg2+ were HAP and β-tricalcium phosphate, respectively.
33955159	6	6	theme	SBF	1287:1289	arg1	flow					1291:1294	The continuous SBF flow	1272:1294	The continuous SBF flow (superficial velocity of 400 μm/s)	1272:1329	The continuous SBF flow (superficial velocity of 400 μm/s) induced the formation of abundant HAP crystals throughout the scaffolds leading to improved mechanical properties to some extent as compared to the initial scaffolds.
33955159	5	7	with	solution	1134:1141	arg1	ratio					1166:1170	[Ca + Mg]/P molar ratio	1148:1170	[Ca + Mg]/P molar ratio of 1.52	1148:1178	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
33955159	1	8	theme	based	252:256	arg1	scaffolds					270:278	alginate based macroporous scaffolds	243:278	alginate based macroporous scaffolds with the aim to imitate the natural bone structure	243:329	In the present study, we synthesized hydroxyapatite (HAP) powders followed by the production of alginate based macroporous scaffolds with the aim to imitate the natural bone structure.
33955159	0	9	theme	hydroxyapatite	93:106	arg1	formation					108:116	Enhanced hydroxyapatite formation	84:116	Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions.	0:145	Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions.
33955159	3	10	theme	macroporous	603:613	arg1	scaffolds					625:633	highly macroporous composite scaffolds	596:633	highly macroporous composite scaffolds	596:633	Upon mixing with Na-alginate, followed by gelation and freeze-dying, highly macroporous composite scaffolds were obtained with open and connected pores and uniformly dispersed mineral phase as determined by scanning electron microscopy.
33955159	6	11	theme	mechanical	1423:1432	arg1	properties					1434:1443	improved mechanical properties	1414:1443	improved mechanical properties	1414:1443	The continuous SBF flow (superficial velocity of 400 μm/s) induced the formation of abundant HAP crystals throughout the scaffolds leading to improved mechanical properties to some extent as compared to the initial scaffolds.
33955159	4	12	theme	Mechanical	764:773	arg1	properties					775:784	Mechanical properties	764:784	Mechanical properties of the scaffolds	764:801	Mechanical properties of the scaffolds were influenced by the composition of calcium phosphate fillers being improved as Ca2+ concentration increased while Mg2+ concentration decreased.
33955159	1	13	theme	macroporous	258:268	arg1	scaffolds					270:278	alginate based macroporous scaffolds	243:278	alginate based macroporous scaffolds with the aim to imitate the natural bone structure	243:329	In the present study, we synthesized hydroxyapatite (HAP) powders followed by the production of alginate based macroporous scaffolds with the aim to imitate the natural bone structure.
33955159	0	14	theme	Enhanced	84:91	arg1	formation					108:116	Enhanced hydroxyapatite formation	84:116	Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions.	0:145	Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions.
33955159	5	15	theme	HAP	1112:1114	arg1	filler					1116:1121	substituted HAP filler	1100:1121	Mg substituted HAP filler	1097:1121	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
33955159	4	16	theme	Ca2+	885:888	arg1	concentration					890:902	Ca2+ concentration	885:902	Ca2+ concentration	885:902	Mechanical properties of the scaffolds were influenced by the composition of calcium phosphate fillers being improved as Ca2+ concentration increased while Mg2+ concentration decreased.
33955159	1	17	theme	scaffolds	270:278	arg1	production					229:238	the production	225:238	the production of alginate based macroporous scaffolds with the aim to imitate the natural bone structure	225:329	In the present study, we synthesized hydroxyapatite (HAP) powders followed by the production of alginate based macroporous scaffolds with the aim to imitate the natural bone structure.
33955159	6	18	theme	HAP	1365:1367	arg1	crystals					1369:1376	abundant HAP crystals	1356:1376	abundant HAP crystals	1356:1376	The continuous SBF flow (superficial velocity of 400 μm/s) induced the formation of abundant HAP crystals throughout the scaffolds leading to improved mechanical properties to some extent as compared to the initial scaffolds.
33955159	4	19	theme	phosphate	849:857	arg1	fillers					859:865	calcium phosphate fillers	841:865	calcium phosphate fillers	841:865	Mechanical properties of the scaffolds were influenced by the composition of calcium phosphate fillers being improved as Ca2+ concentration increased while Mg2+ concentration decreased.
33955159	5	20	theme	simulated	1005:1013	arg1	SBF					1027:1029	SBF	1027:1029	SBF	1027:1029	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
33955159	5	20	theme	simulated	1005:1013	arg1	fluid					1020:1024	simulated body fluid	1005:1024	simulated body fluid (SBF) during 28 days	1005:1045	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
33955159	1	21	theme	hydroxyapatite	184:197	arg1	powders					205:211	hydroxyapatite (HAP) powders	184:211	hydroxyapatite (HAP) powders followed by the production of alginate based macroporous scaffolds with the aim to imitate the natural bone structure	184:329	In the present study, we synthesized hydroxyapatite (HAP) powders followed by the production of alginate based macroporous scaffolds with the aim to imitate the natural bone structure.
33955159	6	22	theme	abundant	1356:1363	arg1	crystals					1369:1376	abundant HAP crystals	1356:1376	abundant HAP crystals	1356:1376	The continuous SBF flow (superficial velocity of 400 μm/s) induced the formation of abundant HAP crystals throughout the scaffolds leading to improved mechanical properties to some extent as compared to the initial scaffolds.
33955159	3	23	theme	mineral	703:709	arg1	phase					711:715	mineral phase as determined by scanning electron microscopy	703:761	mineral phase as determined by scanning electron microscopy	703:761	Upon mixing with Na-alginate, followed by gelation and freeze-dying, highly macroporous composite scaffolds were obtained with open and connected pores and uniformly dispersed mineral phase as determined by scanning electron microscopy.
33955159	4	24	theme	calcium	841:847	arg1	fillers					859:865	calcium phosphate fillers	841:865	calcium phosphate fillers	841:865	Mechanical properties of the scaffolds were influenced by the composition of calcium phosphate fillers being improved as Ca2+ concentration increased while Mg2+ concentration decreased.
33955159	5	25	theme	body	1015:1018	arg1	SBF					1027:1029	SBF	1027:1029	SBF	1027:1029	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
33955159	5	25	theme	body	1015:1018	arg1	fluid					1020:1024	simulated body fluid	1005:1024	simulated body fluid (SBF) during 28 days	1005:1045	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
33955159	7	26	from	use	1568:1570	arg1	engineering					1587:1597	bone tissue engineering	1575:1597	bone tissue engineering	1575:1597	These findings indicated potentials of novel biomimetic scaffolds for use in bone tissue engineering.
33955159	0	27	theme	composite	6:14	arg1	scaffolds					16:24	Novel composite scaffolds	0:24	Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions.	0:145	Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions.
33955159	5	28	theme	precursor	1124:1132	arg1	solution					1134:1141	precursor solution	1124:1141	precursor solution with [Ca + Mg]/P molar ratio of 1.52	1124:1178	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
33955159	5	29	theme	1.52	1175:1178	arg1	ratio					1166:1170	[Ca + Mg]/P molar ratio	1148:1170	[Ca + Mg]/P molar ratio of 1.52	1148:1178	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
33955159	2	30	theme	hydrothermal	372:383	arg1	method					385:390	a hydrothermal method	370:390	a hydrothermal method	370:390	HAP powders were synthesized by using a hydrothermal method, and after calcination, dominant phases in the powders, undoped and doped with Mg2+ were HAP and β-tricalcium phosphate, respectively.
33955159	0	31	theme	Novel	0:4	arg1	scaffolds					16:24	Novel composite scaffolds	0:24	Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions.	0:145	Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions.
33955159	3	32	theme	electron	743:750	arg1	microscopy					752:761	scanning electron microscopy	734:761	scanning electron microscopy	734:761	Upon mixing with Na-alginate, followed by gelation and freeze-dying, highly macroporous composite scaffolds were obtained with open and connected pores and uniformly dispersed mineral phase as determined by scanning electron microscopy.
33955159	4	33	theme	fillers	859:865	arg1	composition					826:836	the composition	822:836	the composition of calcium phosphate fillers	822:865	Mechanical properties of the scaffolds were influenced by the composition of calcium phosphate fillers being improved as Ca2+ concentration increased while Mg2+ concentration decreased.
33955159	6	34	theme	continuous	1276:1285	arg1	flow					1291:1294	The continuous SBF flow	1272:1294	The continuous SBF flow (superficial velocity of 400 μm/s)	1272:1329	The continuous SBF flow (superficial velocity of 400 μm/s) induced the formation of abundant HAP crystals throughout the scaffolds leading to improved mechanical properties to some extent as compared to the initial scaffolds.
33955159	6	35	dep	flow	1291:1294	arg1	velocity					1309:1316	superficial velocity	1297:1316	superficial velocity of 400 μm/s	1297:1328	The continuous SBF flow (superficial velocity of 400 μm/s) induced the formation of abundant HAP crystals throughout the scaffolds leading to improved mechanical properties to some extent as compared to the initial scaffolds.
33955159	2	36	theme	dominant	416:423	arg1	phases					425:430	dominant phases	416:430	dominant phases in the powders, undoped and doped with Mg2+	416:474	HAP powders were synthesized by using a hydrothermal method, and after calcination, dominant phases in the powders, undoped and doped with Mg2+ were HAP and β-tricalcium phosphate, respectively.
33955159	0	37	theme	biomimetic	124:133	arg1	conditions					135:144	biomimetic conditions	124:144	biomimetic conditions	124:144	Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions.
33955159	7	38	theme	scaffolds	1554:1562	arg1	potentials					1523:1532	potentials	1523:1532	potentials of novel biomimetic scaffolds for use in bone tissue engineering	1523:1597	These findings indicated potentials of novel biomimetic scaffolds for use in bone tissue engineering.
33955159	0	39	theme	alginate	35:42	arg1	fillers					75:81	alginate and Mg-doped calcium phosphate fillers	35:81	alginate and Mg-doped calcium phosphate fillers	35:81	Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions.
33955159	3	40	theme	scanning	734:741	arg1	microscopy					752:761	scanning electron microscopy	734:761	scanning electron microscopy	734:761	Upon mixing with Na-alginate, followed by gelation and freeze-dying, highly macroporous composite scaffolds were obtained with open and connected pores and uniformly dispersed mineral phase as determined by scanning electron microscopy.
33955159	7	41	theme	biomimetic	1543:1552	arg1	scaffolds					1554:1562	novel biomimetic scaffolds	1537:1562	novel biomimetic scaffolds	1537:1562	These findings indicated potentials of novel biomimetic scaffolds for use in bone tissue engineering.
33955159	5	42	theme	/P	1157:1158	arg1	ratio					1166:1170	[Ca + Mg]/P molar ratio	1148:1170	[Ca + Mg]/P molar ratio of 1.52	1148:1178	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
33955159	3	43	theme	composite	615:623	arg1	scaffolds					625:633	highly macroporous composite scaffolds	596:633	highly macroporous composite scaffolds	596:633	Upon mixing with Na-alginate, followed by gelation and freeze-dying, highly macroporous composite scaffolds were obtained with open and connected pores and uniformly dispersed mineral phase as determined by scanning electron microscopy.
33955159	5	44	dep	candidate	1086:1094	arg1	Mg					1097:1098	Mg	1097:1098	Mg substituted HAP filler	1097:1121	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
33955159	0	45	theme	Mg-doped	48:55	arg1	fillers					75:81	alginate and Mg-doped calcium phosphate fillers	35:81	alginate and Mg-doped calcium phosphate fillers	35:81	Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions.
33955159	5	46	theme	molar	1160:1164	arg1	ratio					1166:1170	[Ca + Mg]/P molar ratio	1148:1170	[Ca + Mg]/P molar ratio of 1.52	1148:1178	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
33955159	5	47	theme	substituted	1100:1110	arg1	filler					1116:1121	substituted HAP filler	1100:1121	Mg substituted HAP filler	1097:1121	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
33955159	1	48	with	scaffolds	270:278	arg1	aim					289:291	the aim to imitate the natural bone structure	285:329	the aim to imitate the natural bone structure	285:329	In the present study, we synthesized hydroxyapatite (HAP) powders followed by the production of alginate based macroporous scaffolds with the aim to imitate the natural bone structure.
33955159	7	49	theme	bone	1575:1578	arg1	engineering					1587:1597	bone tissue engineering	1575:1597	bone tissue engineering	1575:1597	These findings indicated potentials of novel biomimetic scaffolds for use in bone tissue engineering.
33955159	6	50	theme	improved	1414:1421	arg1	properties					1434:1443	improved mechanical properties	1414:1443	improved mechanical properties	1414:1443	The continuous SBF flow (superficial velocity of 400 μm/s) induced the formation of abundant HAP crystals throughout the scaffolds leading to improved mechanical properties to some extent as compared to the initial scaffolds.
33955159	1	51	theme	natural	308:314	arg1	structure					321:329	the natural bone structure	304:329	the natural bone structure	304:329	In the present study, we synthesized hydroxyapatite (HAP) powders followed by the production of alginate based macroporous scaffolds with the aim to imitate the natural bone structure.
33955159	6	52	theme	400 μm/s	1321:1328	arg1	velocity					1309:1316	superficial velocity	1297:1316	superficial velocity of 400 μm/s	1297:1328	The continuous SBF flow (superficial velocity of 400 μm/s) induced the formation of abundant HAP crystals throughout the scaffolds leading to improved mechanical properties to some extent as compared to the initial scaffolds.
33955159	7	53	theme	novel	1537:1541	arg1	scaffolds					1554:1562	novel biomimetic scaffolds	1537:1562	novel biomimetic scaffolds	1537:1562	These findings indicated potentials of novel biomimetic scaffolds for use in bone tissue engineering.
33955159	5	54	theme	static	1053:1058	arg1	conditions					1060:1069	static conditions	1053:1069	static conditions	1053:1069	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
33955159	2	55	theme	HAP	332:334	arg1	powders					336:342	HAP powders	332:342	HAP powders	332:342	HAP powders were synthesized by using a hydrothermal method, and after calcination, dominant phases in the powders, undoped and doped with Mg2+ were HAP and β-tricalcium phosphate, respectively.
33955159	1	56	theme	bone	316:319	arg1	structure					321:329	the natural bone structure	304:329	the natural bone structure	304:329	In the present study, we synthesized hydroxyapatite (HAP) powders followed by the production of alginate based macroporous scaffolds with the aim to imitate the natural bone structure.
33955159	3	57	theme	connected	663:671	arg1	pores					673:677	connected pores	663:677	connected pores	663:677	Upon mixing with Na-alginate, followed by gelation and freeze-dying, highly macroporous composite scaffolds were obtained with open and connected pores and uniformly dispersed mineral phase as determined by scanning electron microscopy.
33955159	4	58	theme	scaffolds	793:801	arg1	properties					775:784	Mechanical properties	764:784	Mechanical properties of the scaffolds	764:801	Mechanical properties of the scaffolds were influenced by the composition of calcium phosphate fillers being improved as Ca2+ concentration increased while Mg2+ concentration decreased.
33955159	5	59	theme	HAP	950:952	arg1	formation					954:962	HAP formation	950:962	HAP formation within all scaffolds	950:983	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
33955159	5	60	dep	Mg	1097:1098	arg1	filler					1116:1121	substituted HAP filler	1100:1121	Mg substituted HAP filler	1097:1121	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
33955159	5	60	dep	Mg	1097:1098	arg1	solution					1134:1141	precursor solution	1124:1141	precursor solution with [Ca + Mg]/P molar ratio of 1.52	1124:1178	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
33955159	4	61	theme	Mg2+	920:923	arg1	concentration					925:937	Mg2+ concentration	920:937	Mg2+ concentration	920:937	Mechanical properties of the scaffolds were influenced by the composition of calcium phosphate fillers being improved as Ca2+ concentration increased while Mg2+ concentration decreased.
33955159	0	62	dep	scaffolds	16:24	arg1	formation					108:116	Enhanced hydroxyapatite formation	84:116	Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions.	0:145	Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions.
33955159	6	63	theme	crystals	1369:1376	arg1	formation					1343:1351	the formation	1339:1351	the formation of abundant HAP crystals	1339:1376	The continuous SBF flow (superficial velocity of 400 μm/s) induced the formation of abundant HAP crystals throughout the scaffolds leading to improved mechanical properties to some extent as compared to the initial scaffolds.
33955159	0	64	theme	phosphate	65:73	arg1	fillers					75:81	alginate and Mg-doped calcium phosphate fillers	35:81	alginate and Mg-doped calcium phosphate fillers	35:81	Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions.
33955159	5	65	theme	best	1081:1084	arg1	candidate					1086:1094	the best candidate	1077:1094	the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52)	1077:1179	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
33955159	7	66	theme	tissue	1580:1585	arg1	engineering					1587:1597	bone tissue engineering	1575:1597	bone tissue engineering	1575:1597	These findings indicated potentials of novel biomimetic scaffolds for use in bone tissue engineering.
33955159	5	67	theme	biomimetic	1239:1248	arg1	bioreactor					1260:1269	a biomimetic perfusion bioreactor	1237:1269	a biomimetic perfusion bioreactor	1237:1269	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
33955159	0	68	theme	calcium	57:63	arg1	fillers					75:81	alginate and Mg-doped calcium phosphate fillers	35:81	alginate and Mg-doped calcium phosphate fillers	35:81	Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions.
33955159	2	69	from	phases	425:430	arg1	powders					439:445	the powders	435:445	the powders	435:445	HAP powders were synthesized by using a hydrothermal method, and after calcination, dominant phases in the powders, undoped and doped with Mg2+ were HAP and β-tricalcium phosphate, respectively.
33955159	5	70	theme	physiological	1209:1221	arg1	conditions					1223:1232	more physiological conditions	1204:1232	more physiological conditions	1204:1232	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
33955159	1	71	theme	present	154:160	arg1	study					162:166	the present study	150:166	the present study	150:166	In the present study, we synthesized hydroxyapatite (HAP) powders followed by the production of alginate based macroporous scaffolds with the aim to imitate the natural bone structure.
33955159	5	72	theme	perfusion	1250:1258	arg1	bioreactor					1260:1269	a biomimetic perfusion bioreactor	1237:1269	a biomimetic perfusion bioreactor	1237:1269	HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor.
32088232	5	0	theme	materials	769:777	arg1	none					735:738	none	735:738	none of the synthesized composite materials	735:777	Thus, none of the synthesized composite materials were cytotoxic and no cell adhesion was observed on the films.
32088232	6	1	theme	alginate	951:958	arg1	matrix					960:965	the alginate matrix	947:965	the alginate matrix	947:965	Water sorption at the body temperature did not suffer strong changes with the incorporation of CNFs into the alginate matrix.
32088232	9	2	contain	possessing	1399:1408	arg2	transparency					1415:1426	more transparency	1410:1426	more transparency	1410:1426	In addition, the composites reinforced with the lowest CNFs content have the advantage of possessing more transparency and lower production costs.
32088232	9	2	contain	possessing	1399:1408	arg1	advantage					1386:1394	the advantage	1382:1394	the advantage of possessing more transparency and lower production costs	1382:1453	In addition, the composites reinforced with the lowest CNFs content have the advantage of possessing more transparency and lower production costs.
32088232	9	2	contain	possessing	1399:1408	arg2	costs					1449:1453	lower production costs	1432:1453	lower production costs	1432:1453	In addition, the composites reinforced with the lowest CNFs content have the advantage of possessing more transparency and lower production costs.
32088232	7	3	theme	dynamic	972:978	arg1	properties					1015:1024	The dynamic mechanical and tensile/compressive properties	968:1024	The dynamic mechanical and tensile/compressive properties of calcium alginate	968:1044	The dynamic mechanical and tensile/compressive properties of calcium alginate significantly improved with the addition of even a very low amount of CNFs.
32088232	1	4	theme	other	189:193	arg1	polymers					209:216	other biodegradable polymers	189:216	other biodegradable polymers	189:216	Alginates are renewable materials with excellent biocompatibility and cost-effectiveness in comparison with other biodegradable polymers.
32088232	9	5	theme	lowest	1357:1362	arg1	content					1369:1375	the lowest CNFs content	1353:1375	the lowest CNFs content	1353:1375	In addition, the composites reinforced with the lowest CNFs content have the advantage of possessing more transparency and lower production costs.
32088232	8	6	theme	tensile	1132:1138	arg1	modulus					1156:1162	the tensile and compression modulus	1128:1162	modulus	1156:1162	Thus, the tensile and compression modulus of the calcium alginate films in the dry and hydrated state increases up to three and six times, respectively, with the addition of 2% w/w CNFs.
32088232	1	7	theme	biodegradable	195:207	arg1	polymers					209:216	other biodegradable polymers	189:216	other biodegradable polymers	189:216	Alginates are renewable materials with excellent biocompatibility and cost-effectiveness in comparison with other biodegradable polymers.
32088232	8	8	theme	hydrated	1209:1216	arg1	state					1218:1222	the dry and hydrated state	1197:1222	the dry and hydrated state	1197:1222	Thus, the tensile and compression modulus of the calcium alginate films in the dry and hydrated state increases up to three and six times, respectively, with the addition of 2% w/w CNFs.
32088232	9	9	theme	CNFs	1364:1367	arg1	content					1369:1375	the lowest CNFs content	1353:1375	the lowest CNFs content	1353:1375	In addition, the composites reinforced with the lowest CNFs content have the advantage of possessing more transparency and lower production costs.
32088232	6	10	theme	strong	896:901	arg1	changes					903:909	strong changes	896:909	strong changes	896:909	Water sorption at the body temperature did not suffer strong changes with the incorporation of CNFs into the alginate matrix.
32088232	1	11	with	comparison	173:182	arg1	polymers					209:216	other biodegradable polymers	189:216	other biodegradable polymers	189:216	Alginates are renewable materials with excellent biocompatibility and cost-effectiveness in comparison with other biodegradable polymers.
32088232	2	12	theme	skin	338:341	arg1	engineering					350:360	skin tissue engineering	338:360	skin tissue engineering	338:360	However, these hydrogels have poor mechanical properties that restrict their applications in biomedical fields such as skin tissue engineering.
32088232	2	13	theme	tissue	343:348	arg1	engineering					350:360	skin tissue engineering	338:360	skin tissue engineering	338:360	However, these hydrogels have poor mechanical properties that restrict their applications in biomedical fields such as skin tissue engineering.
32088232	3	14	theme	alginate-based	438:451	arg1	films					453:457	alginate-based films	438:457	alginate-based films reinforced with different amounts (0, 0.1, 0.5, 1 and 2% w/w) of carbon nanofibers (CNFs)	438:547	In this regard, the study follows an enhanced engineering route to produce alginate-based films reinforced with different amounts (0, 0.1, 0.5, 1 and 2% w/w) of carbon nanofibers (CNFs) and characterize their physical and biological properties.
32088232	3	15	theme	enhanced	400:407	arg1	route					421:425	an enhanced engineering route	397:425	an enhanced engineering route to produce alginate-based films reinforced with different amounts (0, 0.1, 0.5, 1 and 2% w/w) of carbon nanofibers (CNFs) and characterize their physical and biological properties	397:605	In this regard, the study follows an enhanced engineering route to produce alginate-based films reinforced with different amounts (0, 0.1, 0.5, 1 and 2% w/w) of carbon nanofibers (CNFs) and characterize their physical and biological properties.
32088232	7	16	theme	CNFs	1116:1119	arg1	CNFs					1116:1119	CNFs	1116:1119	CNFs	1116:1119	The dynamic mechanical and tensile/compressive properties of calcium alginate significantly improved with the addition of even a very low amount of CNFs.
32088232	7	16	theme	CNFs	1116:1119	arg1	amount					1106:1111	even a very low amount	1090:1111	even a very low amount of CNFs	1090:1119	The dynamic mechanical and tensile/compressive properties of calcium alginate significantly improved with the addition of even a very low amount of CNFs.
32088232	4	17	theme	neat	704:707	arg1	hydrogels					718:726	neat alginate hydrogels	704:726	neat alginate hydrogels	704:726	The results of this study showed that these composites possess similar biological properties to neat alginate hydrogels.
32088232	7	18	theme	low	1102:1104	arg1	CNFs					1116:1119	CNFs	1116:1119	CNFs	1116:1119	The dynamic mechanical and tensile/compressive properties of calcium alginate significantly improved with the addition of even a very low amount of CNFs.
32088232	7	18	theme	low	1102:1104	arg1	amount					1106:1111	even a very low amount	1090:1111	even a very low amount of CNFs	1090:1119	The dynamic mechanical and tensile/compressive properties of calcium alginate significantly improved with the addition of even a very low amount of CNFs.
32088232	5	19	located	observed	819:826	arg1	films					835:839	the films	831:839	the films	831:839	Thus, none of the synthesized composite materials were cytotoxic and no cell adhesion was observed on the films.
32088232	5	19	located	observed	819:826	arg2	adhesion					806:813	no cell adhesion	798:813	no cell adhesion	798:813	Thus, none of the synthesized composite materials were cytotoxic and no cell adhesion was observed on the films.
32088232	3	20	theme	carbon	524:529	arg1	CNFs					543:546	CNFs	543:546	CNFs	543:546	In this regard, the study follows an enhanced engineering route to produce alginate-based films reinforced with different amounts (0, 0.1, 0.5, 1 and 2% w/w) of carbon nanofibers (CNFs) and characterize their physical and biological properties.
32088232	3	20	theme	carbon	524:529	arg1	nanofibers					531:540	carbon nanofibers	524:540	carbon nanofibers (CNFs)	524:547	In this regard, the study follows an enhanced engineering route to produce alginate-based films reinforced with different amounts (0, 0.1, 0.5, 1 and 2% w/w) of carbon nanofibers (CNFs) and characterize their physical and biological properties.
32088232	4	21	theme	similar	671:677	arg1	properties					690:699	similar biological properties	671:699	similar biological properties	671:699	The results of this study showed that these composites possess similar biological properties to neat alginate hydrogels.
32088232	8	22	theme	2	1296:1296	arg1	%					1297:1297	%	1297:1297	%	1297:1297	Thus, the tensile and compression modulus of the calcium alginate films in the dry and hydrated state increases up to three and six times, respectively, with the addition of 2% w/w CNFs.
32088232	5	23	theme	cell	801:804	arg1	adhesion					806:813	no cell adhesion	798:813	no cell adhesion	798:813	Thus, none of the synthesized composite materials were cytotoxic and no cell adhesion was observed on the films.
32088232	7	24	theme	amount	1106:1111	arg1	addition					1078:1085	the addition	1074:1085	the addition of even a very low amount of CNFs	1074:1119	The dynamic mechanical and tensile/compressive properties of calcium alginate significantly improved with the addition of even a very low amount of CNFs.
32088232	9	25	contain	have	1377:1380	arg1	composites					1326:1335	the composites	1322:1335	the composites reinforced with the lowest CNFs content	1322:1375	In addition, the composites reinforced with the lowest CNFs content have the advantage of possessing more transparency and lower production costs.
32088232	9	25	contain	have	1377:1380	arg2	advantage					1386:1394	the advantage	1382:1394	the advantage of possessing more transparency and lower production costs	1382:1453	In addition, the composites reinforced with the lowest CNFs content have the advantage of possessing more transparency and lower production costs.
32088232	3	26	theme	nanofibers	531:540	arg1	CNFs					543:546	CNFs	543:546	CNFs	543:546	In this regard, the study follows an enhanced engineering route to produce alginate-based films reinforced with different amounts (0, 0.1, 0.5, 1 and 2% w/w) of carbon nanofibers (CNFs) and characterize their physical and biological properties.
32088232	3	26	theme	nanofibers	531:540	arg1	amounts					485:491	different amounts	475:491	different amounts (0, 0.1, 0.5, 1 and 2% w/w) of carbon nanofibers (CNFs)	475:547	In this regard, the study follows an enhanced engineering route to produce alginate-based films reinforced with different amounts (0, 0.1, 0.5, 1 and 2% w/w) of carbon nanofibers (CNFs) and characterize their physical and biological properties.
32088232	3	26	theme	nanofibers	531:540	arg1	%					514:514	0, 0.1, 0.5, 1 and 2% w/w	494:518	0, 0.1, 0.5, 1 and 2% w/w	494:518	In this regard, the study follows an enhanced engineering route to produce alginate-based films reinforced with different amounts (0, 0.1, 0.5, 1 and 2% w/w) of carbon nanofibers (CNFs) and characterize their physical and biological properties.
32088232	3	26	theme	nanofibers	531:540	arg1	nanofibers					531:540	carbon nanofibers	524:540	carbon nanofibers (CNFs)	524:547	In this regard, the study follows an enhanced engineering route to produce alginate-based films reinforced with different amounts (0, 0.1, 0.5, 1 and 2% w/w) of carbon nanofibers (CNFs) and characterize their physical and biological properties.
32088232	8	27	theme	alginate	1179:1186	arg1	films					1188:1192	the calcium alginate films	1167:1192	the calcium alginate films	1167:1192	Thus, the tensile and compression modulus of the calcium alginate films in the dry and hydrated state increases up to three and six times, respectively, with the addition of 2% w/w CNFs.
32088232	8	28	theme	calcium	1171:1177	arg1	films					1188:1192	the calcium alginate films	1167:1192	the calcium alginate films	1167:1192	Thus, the tensile and compression modulus of the calcium alginate films in the dry and hydrated state increases up to three and six times, respectively, with the addition of 2% w/w CNFs.
32088232	1	29	theme	renewable	95:103	arg1	Alginates					81:89	Alginates	81:89	Alginates	81:89	Alginates are renewable materials with excellent biocompatibility and cost-effectiveness in comparison with other biodegradable polymers.
32088232	1	29	theme	renewable	95:103	arg1	materials					105:113	renewable materials	95:113	renewable materials with excellent biocompatibility and cost-effectiveness	95:168	Alginates are renewable materials with excellent biocompatibility and cost-effectiveness in comparison with other biodegradable polymers.
32088232	3	30	theme	different	475:483	arg1	CNFs					543:546	CNFs	543:546	CNFs	543:546	In this regard, the study follows an enhanced engineering route to produce alginate-based films reinforced with different amounts (0, 0.1, 0.5, 1 and 2% w/w) of carbon nanofibers (CNFs) and characterize their physical and biological properties.
32088232	3	30	theme	different	475:483	arg1	amounts					485:491	different amounts	475:491	different amounts (0, 0.1, 0.5, 1 and 2% w/w) of carbon nanofibers (CNFs)	475:547	In this regard, the study follows an enhanced engineering route to produce alginate-based films reinforced with different amounts (0, 0.1, 0.5, 1 and 2% w/w) of carbon nanofibers (CNFs) and characterize their physical and biological properties.
32088232	3	30	theme	different	475:483	arg1	%					514:514	0, 0.1, 0.5, 1 and 2% w/w	494:518	0, 0.1, 0.5, 1 and 2% w/w	494:518	In this regard, the study follows an enhanced engineering route to produce alginate-based films reinforced with different amounts (0, 0.1, 0.5, 1 and 2% w/w) of carbon nanofibers (CNFs) and characterize their physical and biological properties.
32088232	3	30	theme	different	475:483	arg1	nanofibers					531:540	carbon nanofibers	524:540	carbon nanofibers (CNFs)	524:547	In this regard, the study follows an enhanced engineering route to produce alginate-based films reinforced with different amounts (0, 0.1, 0.5, 1 and 2% w/w) of carbon nanofibers (CNFs) and characterize their physical and biological properties.
32088232	0	31	theme	Physical	0:7	arg1	properties					24:33	Physical and biological properties	0:33	Physical and biological properties of alginate/carbon nanofibers hydrogel films	0:78	Physical and biological properties of alginate/carbon nanofibers hydrogel films.
32088232	4	32	contain	possess	663:669	arg1	composites					652:661	these composites	646:661	these composites	646:661	The results of this study showed that these composites possess similar biological properties to neat alginate hydrogels.
32088232	4	32	contain	possess	663:669	arg2	properties					690:699	similar biological properties	671:699	similar biological properties	671:699	The results of this study showed that these composites possess similar biological properties to neat alginate hydrogels.
32088232	3	33	theme	engineering	409:419	arg1	route					421:425	an enhanced engineering route	397:425	an enhanced engineering route to produce alginate-based films reinforced with different amounts (0, 0.1, 0.5, 1 and 2% w/w) of carbon nanofibers (CNFs) and characterize their physical and biological properties	397:605	In this regard, the study follows an enhanced engineering route to produce alginate-based films reinforced with different amounts (0, 0.1, 0.5, 1 and 2% w/w) of carbon nanofibers (CNFs) and characterize their physical and biological properties.
32088232	8	34	theme	dry	1201:1203	arg1	state					1218:1222	the dry and hydrated state	1197:1222	the dry and hydrated state	1197:1222	Thus, the tensile and compression modulus of the calcium alginate films in the dry and hydrated state increases up to three and six times, respectively, with the addition of 2% w/w CNFs.
32088232	4	35	theme	study	628:632	arg1	results					612:618	The results	608:618	The results of this study	608:632	The results of this study showed that these composites possess similar biological properties to neat alginate hydrogels.
32088232	1	36	with	materials	105:113	arg1	cost-effectiveness					151:168	cost-effectiveness	151:168	cost-effectiveness	151:168	Alginates are renewable materials with excellent biocompatibility and cost-effectiveness in comparison with other biodegradable polymers.
32088232	1	36	with	materials	105:113	arg1	biocompatibility					130:145	excellent biocompatibility	120:145	excellent biocompatibility	120:145	Alginates are renewable materials with excellent biocompatibility and cost-effectiveness in comparison with other biodegradable polymers.
32088232	6	37	theme	Water	842:846	arg1	sorption					848:855	Water sorption	842:855	Water sorption at the body temperature	842:879	Water sorption at the body temperature did not suffer strong changes with the incorporation of CNFs into the alginate matrix.
32088232	6	38	theme	body	864:867	arg1	temperature					869:879	the body temperature	860:879	the body temperature	860:879	Water sorption at the body temperature did not suffer strong changes with the incorporation of CNFs into the alginate matrix.
32088232	1	39	theme	excellent	120:128	arg1	biocompatibility					130:145	excellent biocompatibility	120:145	excellent biocompatibility	120:145	Alginates are renewable materials with excellent biocompatibility and cost-effectiveness in comparison with other biodegradable polymers.
32088232	0	40	theme	alginate/carbon	38:52	arg1	films					74:78	alginate/carbon nanofibers hydrogel films	38:78	alginate/carbon nanofibers hydrogel films	38:78	Physical and biological properties of alginate/carbon nanofibers hydrogel films.
32088232	3	41	theme	biological	585:594	arg1	properties					596:605	their physical and biological properties	566:605	their physical and biological properties	566:605	In this regard, the study follows an enhanced engineering route to produce alginate-based films reinforced with different amounts (0, 0.1, 0.5, 1 and 2% w/w) of carbon nanofibers (CNFs) and characterize their physical and biological properties.
32088232	8	42	theme	films	1188:1192	arg1	modulus					1156:1162	the tensile and compression modulus	1128:1162	modulus	1156:1162	Thus, the tensile and compression modulus of the calcium alginate films in the dry and hydrated state increases up to three and six times, respectively, with the addition of 2% w/w CNFs.
32088232	2	43	contain	have	244:247	arg1	hydrogels					234:242	these hydrogels	228:242	these hydrogels	228:242	However, these hydrogels have poor mechanical properties that restrict their applications in biomedical fields such as skin tissue engineering.
32088232	2	43	contain	have	244:247	arg2	properties					265:274	poor mechanical properties	249:274	poor mechanical properties that restrict their applications in biomedical fields such as skin tissue engineering	249:360	However, these hydrogels have poor mechanical properties that restrict their applications in biomedical fields such as skin tissue engineering.
32088232	9	44	theme	lower	1432:1436	arg1	costs					1449:1453	lower production costs	1432:1453	lower production costs	1432:1453	In addition, the composites reinforced with the lowest CNFs content have the advantage of possessing more transparency and lower production costs.
32088232	0	45	theme	hydrogel	65:72	arg1	films					74:78	alginate/carbon nanofibers hydrogel films	38:78	alginate/carbon nanofibers hydrogel films	38:78	Physical and biological properties of alginate/carbon nanofibers hydrogel films.
32088232	6	46	theme	CNFs	937:940	arg1	incorporation					920:932	the incorporation	916:932	the incorporation of CNFs into the alginate matrix	916:965	Water sorption at the body temperature did not suffer strong changes with the incorporation of CNFs into the alginate matrix.
32088232	5	47	theme	composite	759:767	arg1	materials					769:777	the synthesized composite materials	743:777	the synthesized composite materials	743:777	Thus, none of the synthesized composite materials were cytotoxic and no cell adhesion was observed on the films.
32088232	3	48	theme	physical	572:579	arg1	properties					596:605	their physical and biological properties	566:605	their physical and biological properties	566:605	In this regard, the study follows an enhanced engineering route to produce alginate-based films reinforced with different amounts (0, 0.1, 0.5, 1 and 2% w/w) of carbon nanofibers (CNFs) and characterize their physical and biological properties.
32088232	7	49	theme	calcium	1029:1035	arg1	alginate					1037:1044	calcium alginate	1029:1044	calcium alginate	1029:1044	The dynamic mechanical and tensile/compressive properties of calcium alginate significantly improved with the addition of even a very low amount of CNFs.
32088232	9	50	theme	production	1438:1447	arg1	costs					1449:1453	lower production costs	1432:1453	lower production costs	1432:1453	In addition, the composites reinforced with the lowest CNFs content have the advantage of possessing more transparency and lower production costs.
32088232	0	51	theme	nanofibers	54:63	arg1	films					74:78	alginate/carbon nanofibers hydrogel films	38:78	alginate/carbon nanofibers hydrogel films	38:78	Physical and biological properties of alginate/carbon nanofibers hydrogel films.
32088232	2	52	theme	poor	249:252	arg1	properties					265:274	poor mechanical properties	249:274	poor mechanical properties that restrict their applications in biomedical fields such as skin tissue engineering	249:360	However, these hydrogels have poor mechanical properties that restrict their applications in biomedical fields such as skin tissue engineering.
32088232	2	53	theme	mechanical	254:263	arg1	properties					265:274	poor mechanical properties	249:274	poor mechanical properties that restrict their applications in biomedical fields such as skin tissue engineering	249:360	However, these hydrogels have poor mechanical properties that restrict their applications in biomedical fields such as skin tissue engineering.
32088232	7	54	theme	tensile/compressive	995:1013	arg1	properties					1015:1024	The dynamic mechanical and tensile/compressive properties	968:1024	The dynamic mechanical and tensile/compressive properties of calcium alginate	968:1044	The dynamic mechanical and tensile/compressive properties of calcium alginate significantly improved with the addition of even a very low amount of CNFs.
32088232	4	55	theme	alginate	709:716	arg1	hydrogels					718:726	neat alginate hydrogels	704:726	neat alginate hydrogels	704:726	The results of this study showed that these composites possess similar biological properties to neat alginate hydrogels.
32088232	7	56	theme	alginate	1037:1044	arg1	properties					1015:1024	The dynamic mechanical and tensile/compressive properties	968:1024	The dynamic mechanical and tensile/compressive properties of calcium alginate	968:1044	The dynamic mechanical and tensile/compressive properties of calcium alginate significantly improved with the addition of even a very low amount of CNFs.
32088232	8	57	theme	compression	1144:1154	arg1	modulus					1156:1162	the tensile and compression modulus	1128:1162	modulus	1156:1162	Thus, the tensile and compression modulus of the calcium alginate films in the dry and hydrated state increases up to three and six times, respectively, with the addition of 2% w/w CNFs.
32088232	2	58	theme	biomedical	312:321	arg1	engineering					350:360	skin tissue engineering	338:360	skin tissue engineering	338:360	However, these hydrogels have poor mechanical properties that restrict their applications in biomedical fields such as skin tissue engineering.
32088232	2	58	theme	biomedical	312:321	arg1	fields					323:328	biomedical fields	312:328	biomedical fields such as skin tissue engineering	312:360	However, these hydrogels have poor mechanical properties that restrict their applications in biomedical fields such as skin tissue engineering.
32088232	8	59	theme	CNFs	1303:1306	arg1	addition					1284:1291	the addition	1280:1291	the addition of 2% w/w CNFs	1280:1306	Thus, the tensile and compression modulus of the calcium alginate films in the dry and hydrated state increases up to three and six times, respectively, with the addition of 2% w/w CNFs.
32088232	8	60	from	modulus	1156:1162	arg1	state					1218:1222	the dry and hydrated state	1197:1222	the dry and hydrated state	1197:1222	Thus, the tensile and compression modulus of the calcium alginate films in the dry and hydrated state increases up to three and six times, respectively, with the addition of 2% w/w CNFs.
32088232	8	61	theme	w/w	1299:1301	arg1	CNFs					1303:1306	2% w/w CNFs	1296:1306	2% w/w CNFs	1296:1306	Thus, the tensile and compression modulus of the calcium alginate films in the dry and hydrated state increases up to three and six times, respectively, with the addition of 2% w/w CNFs.
32088232	7	62	theme	mechanical	980:989	arg1	properties					1015:1024	The dynamic mechanical and tensile/compressive properties	968:1024	The dynamic mechanical and tensile/compressive properties of calcium alginate	968:1044	The dynamic mechanical and tensile/compressive properties of calcium alginate significantly improved with the addition of even a very low amount of CNFs.
32088232	8	63	theme	%	1297:1297	arg1	CNFs					1303:1306	2% w/w CNFs	1296:1306	2% w/w CNFs	1296:1306	Thus, the tensile and compression modulus of the calcium alginate films in the dry and hydrated state increases up to three and six times, respectively, with the addition of 2% w/w CNFs.
32088232	5	64	theme	synthesized	747:757	arg1	materials					769:777	the synthesized composite materials	743:777	the synthesized composite materials	743:777	Thus, none of the synthesized composite materials were cytotoxic and no cell adhesion was observed on the films.
32088232	9	65	theme	more	1410:1413	arg1	transparency					1415:1426	more transparency	1410:1426	more transparency	1410:1426	In addition, the composites reinforced with the lowest CNFs content have the advantage of possessing more transparency and lower production costs.
32088232	1	66	from	materials	105:113	arg1	comparison					173:182	comparison	173:182	comparison with other biodegradable polymers	173:216	Alginates are renewable materials with excellent biocompatibility and cost-effectiveness in comparison with other biodegradable polymers.
32088232	0	67	theme	films	74:78	arg1	properties					24:33	Physical and biological properties	0:33	Physical and biological properties of alginate/carbon nanofibers hydrogel films	0:78	Physical and biological properties of alginate/carbon nanofibers hydrogel films.
32088232	0	68	theme	biological	13:22	arg1	properties					24:33	Physical and biological properties	0:33	Physical and biological properties of alginate/carbon nanofibers hydrogel films	0:78	Physical and biological properties of alginate/carbon nanofibers hydrogel films.
32088232	4	69	theme	biological	679:688	arg1	properties					690:699	similar biological properties	671:699	similar biological properties	671:699	The results of this study showed that these composites possess similar biological properties to neat alginate hydrogels.
32088232	6	70	from	temperature	869:879	arg1	sorption					848:855	Water sorption	842:855	Water sorption at the body temperature	842:879	Water sorption at the body temperature did not suffer strong changes with the incorporation of CNFs into the alginate matrix.
33011479	7	0	theme	ng	1475:1476	arg1	L-1					1478:1480	10-20 (15.6) ng L-1	1462:1480	10-20 (15.6) ng L-1	1462:1480	The quantitation of MP, EP, PP, and BP was made at detection range (and limits of detection (LOD)) of 5-15 (9.8), 11-21 (16.2), 6-18 (12.4), and 10-20 (15.6) ng L-1, respectively.
33011479	3	1	theme	coral	638:642	arg1	structure					654:662	coral reef-like structure	638:662	coral reef-like structure	638:662	CS-Ni/Fe LDH composites with a heterogeneous, porous texture, and coral reef-like structure exhibit appealing extraction efficiency for the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions.
33011479	4	2	theme	composites	851:860	arg1	performance					832:842	The performance	828:842	The performance of the composites	828:860	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	7	3	theme	PP	1345:1346	arg1	quantitation					1321:1332	The quantitation	1317:1332	The quantitation of MP, EP, PP, and BP	1317:1354	The quantitation of MP, EP, PP, and BP was made at detection range (and limits of detection (LOD)) of 5-15 (9.8), 11-21 (16.2), 6-18 (12.4), and 10-20 (15.6) ng L-1, respectively.
33011479	5	4	theme	wide	1063:1066	arg1	range					1075:1079	a wide linear range	1061:1079	a wide linear range (e.g., 100-50,000 ng L-1)	1061:1105	The LDH-SPE-HPLC method exhibited a wide linear range (e.g., 100-50,000 ng L-1), good linearity (R2 ≥ 0.999), and good precision (relative standard deviation (RSD) < 3%).
33011479	5	4	theme	wide	1063:1066	arg1	100-50,000 ng L-1					1088:1104	100-50,000 ng L-1	1088:1104	100-50,000 ng L-1	1088:1104	The LDH-SPE-HPLC method exhibited a wide linear range (e.g., 100-50,000 ng L-1), good linearity (R2 ≥ 0.999), and good precision (relative standard deviation (RSD) < 3%).
33011479	2	5	theme	sorbent	536:542	arg1	extraction					525:534	solid phase extraction	513:534	solid phase extraction sorbent prior to HPLC-PDA analysis	513:569	In this research, we focused on quantification of four parabens (i.e., methylparaben (MP), ethylparaben (EP), propylparaben (PP), and butyl paraben (BP)) using chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites as solid phase extraction sorbent prior to HPLC-PDA analysis.
33011479	3	6	theme	LDH	581:583	arg1	composites					585:594	CS-Ni/Fe LDH composites	572:594	CS-Ni/Fe LDH composites with a heterogeneous, porous texture, and coral reef-like structure	572:662	CS-Ni/Fe LDH composites with a heterogeneous, porous texture, and coral reef-like structure exhibit appealing extraction efficiency for the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions.
33011479	5	7	theme	standard	1166:1173	arg1	RSD					1186:1188	RSD	1186:1188	RSD	1186:1188	The LDH-SPE-HPLC method exhibited a wide linear range (e.g., 100-50,000 ng L-1), good linearity (R2 ≥ 0.999), and good precision (relative standard deviation (RSD) < 3%).
33011479	5	7	theme	standard	1166:1173	arg1	deviation					1175:1183	relative standard deviation	1157:1183	relative standard deviation (RSD) < 3%	1157:1194	The LDH-SPE-HPLC method exhibited a wide linear range (e.g., 100-50,000 ng L-1), good linearity (R2 ≥ 0.999), and good precision (relative standard deviation (RSD) < 3%).
33011479	3	8	theme	hydrogen	781:788	arg1	bonding					790:796	hydrogen bonding	781:796	hydrogen bonding	781:796	CS-Ni/Fe LDH composites with a heterogeneous, porous texture, and coral reef-like structure exhibit appealing extraction efficiency for the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions.
33011479	0	9	theme	HPLC-PDA	102:109	arg1	monitoring					111:120	HPLC-PDA monitoring	102:120	HPLC-PDA monitoring of parabens in personal care products	102:158	Chitosan-Ni/Fe layered double hydroxide composites as an efficient solid phase extraction sorbent for HPLC-PDA monitoring of parabens in personal care products.
33011479	5	10	theme	linear	1068:1073	arg1	range					1075:1079	a wide linear range	1061:1079	a wide linear range (e.g., 100-50,000 ng L-1)	1061:1105	The LDH-SPE-HPLC method exhibited a wide linear range (e.g., 100-50,000 ng L-1), good linearity (R2 ≥ 0.999), and good precision (relative standard deviation (RSD) < 3%).
33011479	5	10	theme	linear	1068:1073	arg1	100-50,000 ng L-1					1088:1104	100-50,000 ng L-1	1088:1104	100-50,000 ng L-1	1088:1104	The LDH-SPE-HPLC method exhibited a wide linear range (e.g., 100-50,000 ng L-1), good linearity (R2 ≥ 0.999), and good precision (relative standard deviation (RSD) < 3%).
33011479	8	11	theme	excellent	1536:1544	arg1	performance					1546:1556	excellent performance	1536:1556	excellent performance	1536:1556	The prepared composites also displayed excellent performance with enhanced reusability/durability (n = 30 cycles) and reproducibility (n = 5).
33011479	2	12	dep	methylparaben	336:348	arg1	i.e.					330:333	i.e.	330:333	i.e.	330:333	In this research, we focused on quantification of four parabens (i.e., methylparaben (MP), ethylparaben (EP), propylparaben (PP), and butyl paraben (BP)) using chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites as solid phase extraction sorbent prior to HPLC-PDA analysis.
33011479	4	13	theme	hair	999:1002	arg1	serum					1004:1008	hair serum	999:1008	hair serum	999:1008	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	13	theme	hair	999:1002	arg1	samples					942:948	standard samples	933:948	standard samples	933:948	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	14	from	samples	959:965	arg1	parabens					919:926	parabens	919:926	parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen)	919:1024	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	14	from	samples	959:965	arg1	extraction					905:914	solid phase extraction	893:914	solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen)	893:1024	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	2	15	theme	nickel/iron	447:457	arg1	composites					499:508	chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites	425:508	chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites	425:508	In this research, we focused on quantification of four parabens (i.e., methylparaben (MP), ethylparaben (EP), propylparaben (PP), and butyl paraben (BP)) using chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites as solid phase extraction sorbent prior to HPLC-PDA analysis.
33011479	2	16	theme	phase	519:523	arg1	extraction					525:534	solid phase extraction	513:534	solid phase extraction sorbent prior to HPLC-PDA analysis	513:569	In this research, we focused on quantification of four parabens (i.e., methylparaben (MP), ethylparaben (EP), propylparaben (PP), and butyl paraben (BP)) using chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites as solid phase extraction sorbent prior to HPLC-PDA analysis.
33011479	8	17	theme	enhanced	1563:1570	arg1	reusability/durability					1572:1593	enhanced reusability/durability	1563:1593	enhanced reusability/durability (n = 30 cycles)	1563:1609	The prepared composites also displayed excellent performance with enhanced reusability/durability (n = 30 cycles) and reproducibility (n = 5).
33011479	8	17	theme	enhanced	1563:1570	arg1	cycles					1603:1608	n = 30 cycles	1596:1608	n = 30 cycles	1596:1608	The prepared composites also displayed excellent performance with enhanced reusability/durability (n = 30 cycles) and reproducibility (n = 5).
33011479	7	18	theme	12.4	1451:1454	arg1	limits					1389:1394	limits	1389:1394	limits of detection (LOD)	1389:1413	The quantitation of MP, EP, PP, and BP was made at detection range (and limits of detection (LOD)) of 5-15 (9.8), 11-21 (16.2), 6-18 (12.4), and 10-20 (15.6) ng L-1, respectively.
33011479	7	18	theme	12.4	1451:1454	arg1	range					1378:1382	detection range	1368:1382	detection range	1368:1382	The quantitation of MP, EP, PP, and BP was made at detection range (and limits of detection (LOD)) of 5-15 (9.8), 11-21 (16.2), 6-18 (12.4), and 10-20 (15.6) ng L-1, respectively.
33011479	6	19	theme	selected	1232:1239	arg1	parabens					1241:1248	selected parabens	1232:1248	selected parabens	1232:1248	This method successfully enriched selected parabens with remarkable recovery above 85.95% and a good RSD (0.01-2.90%).
33011479	2	20	theme	intercalated	434:445	arg1	composites					499:508	chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites	425:508	chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites	425:508	In this research, we focused on quantification of four parabens (i.e., methylparaben (MP), ethylparaben (EP), propylparaben (PP), and butyl paraben (BP)) using chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites as solid phase extraction sorbent prior to HPLC-PDA analysis.
33011479	3	21	theme	appealing	672:680	arg1	efficiency					693:702	appealing extraction efficiency	672:702	appealing extraction efficiency for the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions	672:825	CS-Ni/Fe LDH composites with a heterogeneous, porous texture, and coral reef-like structure exhibit appealing extraction efficiency for the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions.
33011479	5	22	theme	relative	1157:1164	arg1	RSD					1186:1188	RSD	1186:1188	RSD	1186:1188	The LDH-SPE-HPLC method exhibited a wide linear range (e.g., 100-50,000 ng L-1), good linearity (R2 ≥ 0.999), and good precision (relative standard deviation (RSD) < 3%).
33011479	5	22	theme	relative	1157:1164	arg1	deviation					1175:1183	relative standard deviation	1157:1183	relative standard deviation (RSD) < 3%	1157:1194	The LDH-SPE-HPLC method exhibited a wide linear range (e.g., 100-50,000 ng L-1), good linearity (R2 ≥ 0.999), and good precision (relative standard deviation (RSD) < 3%).
33011479	7	23	theme	9.8	1425:1427	arg1	limits					1389:1394	limits	1389:1394	limits of detection (LOD)	1389:1413	The quantitation of MP, EP, PP, and BP was made at detection range (and limits of detection (LOD)) of 5-15 (9.8), 11-21 (16.2), 6-18 (12.4), and 10-20 (15.6) ng L-1, respectively.
33011479	7	23	theme	9.8	1425:1427	arg1	range					1378:1382	detection range	1368:1382	detection range	1368:1382	The quantitation of MP, EP, PP, and BP was made at detection range (and limits of detection (LOD)) of 5-15 (9.8), 11-21 (16.2), 6-18 (12.4), and 10-20 (15.6) ng L-1, respectively.
33011479	2	24	theme	chitosan	425:432	arg1	composites					499:508	chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites	425:508	chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites	425:508	In this research, we focused on quantification of four parabens (i.e., methylparaben (MP), ethylparaben (EP), propylparaben (PP), and butyl paraben (BP)) using chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites as solid phase extraction sorbent prior to HPLC-PDA analysis.
33011479	3	25	theme	reef-like	644:652	arg1	structure					654:662	coral reef-like structure	638:662	coral reef-like structure	638:662	CS-Ni/Fe LDH composites with a heterogeneous, porous texture, and coral reef-like structure exhibit appealing extraction efficiency for the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions.
33011479	0	26	theme	Chitosan-Ni/Fe	0:13	arg1	composites					40:49	Chitosan-Ni/Fe layered double hydroxide composites	0:49	Chitosan-Ni/Fe layered double hydroxide composites as an efficient solid phase extraction	0:88	Chitosan-Ni/Fe layered double hydroxide composites as an efficient solid phase extraction sorbent for HPLC-PDA monitoring of parabens in personal care products.
33011479	4	27	theme	solid	893:897	arg1	extraction					905:914	solid phase extraction	893:914	solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen)	893:1024	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	1	28	theme	dire	172:175	arg1	need					177:180	a dire need	170:180	a dire need for development of efficient and sensitive methods to efficiently screen parabens	170:262	There is a dire need for development of efficient and sensitive methods to efficiently screen parabens.
33011479	0	29	theme	layered	15:21	arg1	composites					40:49	Chitosan-Ni/Fe layered double hydroxide composites	0:49	Chitosan-Ni/Fe layered double hydroxide composites as an efficient solid phase extraction	0:88	Chitosan-Ni/Fe layered double hydroxide composites as an efficient solid phase extraction sorbent for HPLC-PDA monitoring of parabens in personal care products.
33011479	2	30	theme	HPLC-PDA	553:560	arg1	analysis					562:569	HPLC-PDA analysis	553:569	HPLC-PDA analysis	553:569	In this research, we focused on quantification of four parabens (i.e., methylparaben (MP), ethylparaben (EP), propylparaben (PP), and butyl paraben (BP)) using chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites as solid phase extraction sorbent prior to HPLC-PDA analysis.
33011479	4	31	theme	standard	933:940	arg1	toothpaste					987:996	toothpaste	987:996	toothpaste	987:996	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	31	theme	standard	933:940	arg1	serum					1004:1008	hair serum	999:1008	hair serum	999:1008	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	31	theme	standard	933:940	arg1	samples					942:948	standard samples	933:948	standard samples	933:948	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	31	theme	standard	933:940	arg1	water					973:977	rose water	968:977	rose water	968:977	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	31	theme	standard	933:940	arg1	cream					980:984	cream	980:984	cream	980:984	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	31	theme	standard	933:940	arg1	sunscreen					1015:1023	sunscreen	1015:1023	sunscreen	1015:1023	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	5	32	dep	deviation	1175:1183	arg1	%					1194:1194	 < 3%	1190:1194	relative standard deviation (RSD) < 3%	1157:1194	The LDH-SPE-HPLC method exhibited a wide linear range (e.g., 100-50,000 ng L-1), good linearity (R2 ≥ 0.999), and good precision (relative standard deviation (RSD) < 3%).
33011479	7	33	theme	MP	1337:1338	arg1	quantitation					1321:1332	The quantitation	1317:1332	The quantitation of MP, EP, PP, and BP	1317:1354	The quantitation of MP, EP, PP, and BP was made at detection range (and limits of detection (LOD)) of 5-15 (9.8), 11-21 (16.2), 6-18 (12.4), and 10-20 (15.6) ng L-1, respectively.
33011479	6	34	theme	good	1294:1297	arg1	RSD					1299:1301	a good RSD	1292:1301	a good RSD (0.01-2.90%)	1292:1314	This method successfully enriched selected parabens with remarkable recovery above 85.95% and a good RSD (0.01-2.90%).
33011479	6	34	theme	good	1294:1297	arg1	%					1313:1313	0.01-2.90%	1304:1313	0.01-2.90%	1304:1313	This method successfully enriched selected parabens with remarkable recovery above 85.95% and a good RSD (0.01-2.90%).
33011479	0	35	theme	care	146:149	arg1	products					151:158	personal care products	137:158	personal care products	137:158	Chitosan-Ni/Fe layered double hydroxide composites as an efficient solid phase extraction sorbent for HPLC-PDA monitoring of parabens in personal care products.
33011479	4	36	theme	rose	968:971	arg1	samples					942:948	standard samples	933:948	standard samples	933:948	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	36	theme	rose	968:971	arg1	water					973:977	rose water	968:977	rose water	968:977	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	6	37	theme	remarkable	1255:1264	arg1	recovery					1266:1273	remarkable recovery	1255:1273	remarkable recovery above 85.95%	1255:1286	This method successfully enriched selected parabens with remarkable recovery above 85.95% and a good RSD (0.01-2.90%).
33011479	0	38	theme	hydroxide	30:38	arg1	composites					40:49	Chitosan-Ni/Fe layered double hydroxide composites	0:49	Chitosan-Ni/Fe layered double hydroxide composites as an efficient solid phase extraction	0:88	Chitosan-Ni/Fe layered double hydroxide composites as an efficient solid phase extraction sorbent for HPLC-PDA monitoring of parabens in personal care products.
33011479	2	39	theme	CS-Ni/Fe-LDH	485:496	arg1	composites					499:508	chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites	425:508	chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites	425:508	In this research, we focused on quantification of four parabens (i.e., methylparaben (MP), ethylparaben (EP), propylparaben (PP), and butyl paraben (BP)) using chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites as solid phase extraction sorbent prior to HPLC-PDA analysis.
33011479	7	40	theme	EP	1341:1342	arg1	quantitation					1321:1332	The quantitation	1317:1332	The quantitation of MP, EP, PP, and BP	1317:1354	The quantitation of MP, EP, PP, and BP was made at detection range (and limits of detection (LOD)) of 5-15 (9.8), 11-21 (16.2), 6-18 (12.4), and 10-20 (15.6) ng L-1, respectively.
33011479	0	41	theme	double	23:28	arg1	composites					40:49	Chitosan-Ni/Fe layered double hydroxide composites	0:49	Chitosan-Ni/Fe layered double hydroxide composites as an efficient solid phase extraction	0:88	Chitosan-Ni/Fe layered double hydroxide composites as an efficient solid phase extraction sorbent for HPLC-PDA monitoring of parabens in personal care products.
33011479	3	42	theme	extraction	682:691	arg1	efficiency					693:702	appealing extraction efficiency	672:702	appealing extraction efficiency for the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions	672:825	CS-Ni/Fe LDH composites with a heterogeneous, porous texture, and coral reef-like structure exhibit appealing extraction efficiency for the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions.
33011479	0	43	theme	parabens	125:132	arg1	monitoring					111:120	HPLC-PDA monitoring	102:120	HPLC-PDA monitoring of parabens in personal care products	102:158	Chitosan-Ni/Fe layered double hydroxide composites as an efficient solid phase extraction sorbent for HPLC-PDA monitoring of parabens in personal care products.
33011479	7	44	theme	detection	1368:1376	arg1	range					1378:1382	detection range	1368:1382	detection range	1368:1382	The quantitation of MP, EP, PP, and BP was made at detection range (and limits of detection (LOD)) of 5-15 (9.8), 11-21 (16.2), 6-18 (12.4), and 10-20 (15.6) ng L-1, respectively.
33011479	3	45	with	composites	585:594	arg1	structure					654:662	coral reef-like structure	638:662	coral reef-like structure	638:662	CS-Ni/Fe LDH composites with a heterogeneous, porous texture, and coral reef-like structure exhibit appealing extraction efficiency for the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions.
33011479	3	45	with	composites	585:594	arg1	texture					625:631	a heterogeneous, porous texture	601:631	a heterogeneous, porous texture	601:631	CS-Ni/Fe LDH composites with a heterogeneous, porous texture, and coral reef-like structure exhibit appealing extraction efficiency for the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions.
33011479	5	46	theme	good	1108:1111	arg1	R2 ≥ 0.999					1124:1133	R2 ≥ 0.999	1124:1133	R2 ≥ 0.999	1124:1133	The LDH-SPE-HPLC method exhibited a wide linear range (e.g., 100-50,000 ng L-1), good linearity (R2 ≥ 0.999), and good precision (relative standard deviation (RSD) < 3%).
33011479	5	46	theme	good	1108:1111	arg1	linearity					1113:1121	good linearity	1108:1121	good linearity (R2 ≥ 0.999)	1108:1134	The LDH-SPE-HPLC method exhibited a wide linear range (e.g., 100-50,000 ng L-1), good linearity (R2 ≥ 0.999), and good precision (relative standard deviation (RSD) < 3%).
33011479	2	47	theme	hydroxide	474:482	arg1	composites					499:508	chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites	425:508	chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites	425:508	In this research, we focused on quantification of four parabens (i.e., methylparaben (MP), ethylparaben (EP), propylparaben (PP), and butyl paraben (BP)) using chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites as solid phase extraction sorbent prior to HPLC-PDA analysis.
33011479	5	48	dep	precision	1146:1154	arg1	RSD					1186:1188	RSD	1186:1188	RSD	1186:1188	The LDH-SPE-HPLC method exhibited a wide linear range (e.g., 100-50,000 ng L-1), good linearity (R2 ≥ 0.999), and good precision (relative standard deviation (RSD) < 3%).
33011479	5	48	dep	precision	1146:1154	arg1	deviation					1175:1183	relative standard deviation	1157:1183	relative standard deviation (RSD) < 3%	1157:1194	The LDH-SPE-HPLC method exhibited a wide linear range (e.g., 100-50,000 ng L-1), good linearity (R2 ≥ 0.999), and good precision (relative standard deviation (RSD) < 3%).
33011479	7	49	theme	16.2	1438:1441	arg1	limits					1389:1394	limits	1389:1394	limits of detection (LOD)	1389:1413	The quantitation of MP, EP, PP, and BP was made at detection range (and limits of detection (LOD)) of 5-15 (9.8), 11-21 (16.2), 6-18 (12.4), and 10-20 (15.6) ng L-1, respectively.
33011479	7	49	theme	16.2	1438:1441	arg1	range					1378:1382	detection range	1368:1382	detection range	1368:1382	The quantitation of MP, EP, PP, and BP was made at detection range (and limits of detection (LOD)) of 5-15 (9.8), 11-21 (16.2), 6-18 (12.4), and 10-20 (15.6) ng L-1, respectively.
33011479	1	50	theme	efficient	201:209	arg1	methods					225:231	efficient and sensitive methods	201:231	efficient and sensitive methods	201:231	There is a dire need for development of efficient and sensitive methods to efficiently screen parabens.
33011479	4	51	theme	real	954:957	arg1	samples					959:965	real samples	954:965	real samples	954:965	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	3	52	theme	due	728:730	arg1	parabens					719:726	the target parabens	708:726	the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions	708:825	CS-Ni/Fe LDH composites with a heterogeneous, porous texture, and coral reef-like structure exhibit appealing extraction efficiency for the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions.
33011479	0	53	theme	personal	137:144	arg1	products					151:158	personal care products	137:158	personal care products	137:158	Chitosan-Ni/Fe layered double hydroxide composites as an efficient solid phase extraction sorbent for HPLC-PDA monitoring of parabens in personal care products.
33011479	3	54	theme	CS-Ni/Fe	572:579	arg1	composites					585:594	CS-Ni/Fe LDH composites	572:594	CS-Ni/Fe LDH composites with a heterogeneous, porous texture, and coral reef-like structure	572:662	CS-Ni/Fe LDH composites with a heterogeneous, porous texture, and coral reef-like structure exhibit appealing extraction efficiency for the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions.
33011479	7	55	theme	detection	1399:1407	arg1	limits					1389:1394	limits	1389:1394	limits of detection (LOD)	1389:1413	The quantitation of MP, EP, PP, and BP was made at detection range (and limits of detection (LOD)) of 5-15 (9.8), 11-21 (16.2), 6-18 (12.4), and 10-20 (15.6) ng L-1, respectively.
33011479	7	55	theme	detection	1399:1407	arg1	range					1378:1382	detection range	1368:1382	detection range	1368:1382	The quantitation of MP, EP, PP, and BP was made at detection range (and limits of detection (LOD)) of 5-15 (9.8), 11-21 (16.2), 6-18 (12.4), and 10-20 (15.6) ng L-1, respectively.
33011479	2	56	theme	butyl	399:403	arg1	BP					414:415	BP	414:415	BP	414:415	In this research, we focused on quantification of four parabens (i.e., methylparaben (MP), ethylparaben (EP), propylparaben (PP), and butyl paraben (BP)) using chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites as solid phase extraction sorbent prior to HPLC-PDA analysis.
33011479	2	56	theme	butyl	399:403	arg1	paraben					405:411	butyl paraben	399:411	butyl paraben (BP)	399:416	In this research, we focused on quantification of four parabens (i.e., methylparaben (MP), ethylparaben (EP), propylparaben (PP), and butyl paraben (BP)) using chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites as solid phase extraction sorbent prior to HPLC-PDA analysis.
33011479	2	57	theme	layered	459:465	arg1	composites					499:508	chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites	425:508	chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites	425:508	In this research, we focused on quantification of four parabens (i.e., methylparaben (MP), ethylparaben (EP), propylparaben (PP), and butyl paraben (BP)) using chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites as solid phase extraction sorbent prior to HPLC-PDA analysis.
33011479	2	58	theme	solid	513:517	arg1	extraction					525:534	solid phase extraction	513:534	solid phase extraction sorbent prior to HPLC-PDA analysis	513:569	In this research, we focused on quantification of four parabens (i.e., methylparaben (MP), ethylparaben (EP), propylparaben (PP), and butyl paraben (BP)) using chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites as solid phase extraction sorbent prior to HPLC-PDA analysis.
33011479	0	59	theme	solid	67:71	arg1	extraction					79:88	an efficient solid phase extraction	54:88	an efficient solid phase extraction	54:88	Chitosan-Ni/Fe layered double hydroxide composites as an efficient solid phase extraction sorbent for HPLC-PDA monitoring of parabens in personal care products.
33011479	5	60	theme	LDH-SPE-HPLC	1031:1042	arg1	method					1044:1049	The LDH-SPE-HPLC method	1027:1049	The LDH-SPE-HPLC method	1027:1049	The LDH-SPE-HPLC method exhibited a wide linear range (e.g., 100-50,000 ng L-1), good linearity (R2 ≥ 0.999), and good precision (relative standard deviation (RSD) < 3%).
33011479	5	61	dep	100-50,000 ng L-1	1088:1104	arg1	e.g.					1082:1085	e.g.	1082:1085	e.g.	1082:1085	The LDH-SPE-HPLC method exhibited a wide linear range (e.g., 100-50,000 ng L-1), good linearity (R2 ≥ 0.999), and good precision (relative standard deviation (RSD) < 3%).
33011479	2	62	theme	double	467:472	arg1	composites					499:508	chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites	425:508	chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites	425:508	In this research, we focused on quantification of four parabens (i.e., methylparaben (MP), ethylparaben (EP), propylparaben (PP), and butyl paraben (BP)) using chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites as solid phase extraction sorbent prior to HPLC-PDA analysis.
33011479	3	63	theme	target	712:717	arg1	parabens					719:726	the target parabens	708:726	the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions	708:825	CS-Ni/Fe LDH composites with a heterogeneous, porous texture, and coral reef-like structure exhibit appealing extraction efficiency for the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions.
33011479	4	64	dep	samples	942:948	arg1	toothpaste					987:996	toothpaste	987:996	toothpaste	987:996	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	64	dep	samples	942:948	arg1	serum					1004:1008	hair serum	999:1008	hair serum	999:1008	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	64	dep	samples	942:948	arg1	samples					942:948	standard samples	933:948	standard samples	933:948	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	64	dep	samples	942:948	arg1	water					973:977	rose water	968:977	rose water	968:977	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	64	dep	samples	942:948	arg1	cream					980:984	cream	980:984	cream	980:984	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	64	dep	samples	942:948	arg1	sunscreen					1015:1023	sunscreen	1015:1023	sunscreen	1015:1023	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	7	65	theme	BP	1353:1354	arg1	quantitation					1321:1332	The quantitation	1317:1332	The quantitation of MP, EP, PP, and BP	1317:1354	The quantitation of MP, EP, PP, and BP was made at detection range (and limits of detection (LOD)) of 5-15 (9.8), 11-21 (16.2), 6-18 (12.4), and 10-20 (15.6) ng L-1, respectively.
33011479	3	66	theme	hydrophobic	802:812	arg1	interactions					814:825	hydrophobic interactions	802:825	hydrophobic interactions	802:825	CS-Ni/Fe LDH composites with a heterogeneous, porous texture, and coral reef-like structure exhibit appealing extraction efficiency for the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions.
33011479	4	67	from	samples	942:948	arg1	parabens					919:926	parabens	919:926	parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen)	919:1024	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	67	from	samples	942:948	arg1	extraction					905:914	solid phase extraction	893:914	solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen)	893:1024	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	0	68	from	monitoring	111:120	arg1	products					151:158	personal care products	137:158	personal care products	137:158	Chitosan-Ni/Fe layered double hydroxide composites as an efficient solid phase extraction sorbent for HPLC-PDA monitoring of parabens in personal care products.
33011479	4	69	from	extraction	905:914	arg1	toothpaste					987:996	toothpaste	987:996	toothpaste	987:996	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	69	from	extraction	905:914	arg1	serum					1004:1008	hair serum	999:1008	hair serum	999:1008	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	69	from	extraction	905:914	arg1	samples					942:948	standard samples	933:948	standard samples	933:948	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	69	from	extraction	905:914	arg1	water					973:977	rose water	968:977	rose water	968:977	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	69	from	extraction	905:914	arg1	cream					980:984	cream	980:984	cream	980:984	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	69	from	extraction	905:914	arg1	sunscreen					1015:1023	sunscreen	1015:1023	sunscreen	1015:1023	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	4	69	from	extraction	905:914	arg1	samples					959:965	real samples	954:965	real samples	954:965	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	2	70	theme	parabens	320:327	arg1	quantification					297:310	quantification	297:310	quantification of four parabens (i.e., methylparaben (MP), ethylparaben (EP), propylparaben (PP), and butyl paraben (BP))	297:417	In this research, we focused on quantification of four parabens (i.e., methylparaben (MP), ethylparaben (EP), propylparaben (PP), and butyl paraben (BP)) using chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites as solid phase extraction sorbent prior to HPLC-PDA analysis.
33011479	3	71	theme	interactions	814:825	arg1	formation					768:776	the formation	764:776	the formation of hydrogen bonding and hydrophobic interactions	764:825	CS-Ni/Fe LDH composites with a heterogeneous, porous texture, and coral reef-like structure exhibit appealing extraction efficiency for the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions.
33011479	1	72	theme	sensitive	215:223	arg1	methods					225:231	efficient and sensitive methods	201:231	efficient and sensitive methods	201:231	There is a dire need for development of efficient and sensitive methods to efficiently screen parabens.
33011479	3	73	dep	heterogeneous	603:615	arg1	porous					618:623	porous	618:623	porous	618:623	CS-Ni/Fe LDH composites with a heterogeneous, porous texture, and coral reef-like structure exhibit appealing extraction efficiency for the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions.
33011479	4	74	theme	phase	899:903	arg1	extraction					905:914	solid phase extraction	893:914	solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen)	893:1024	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	3	75	theme	bonding	790:796	arg1	formation					768:776	the formation	764:776	the formation of hydrogen bonding and hydrophobic interactions	764:825	CS-Ni/Fe LDH composites with a heterogeneous, porous texture, and coral reef-like structure exhibit appealing extraction efficiency for the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions.
33011479	1	76	theme	methods	225:231	arg1	development					186:196	development	186:196	development of efficient and sensitive methods	186:231	There is a dire need for development of efficient and sensitive methods to efficiently screen parabens.
33011479	0	77	theme	phase	73:77	arg1	extraction					79:88	an efficient solid phase extraction	54:88	an efficient solid phase extraction	54:88	Chitosan-Ni/Fe layered double hydroxide composites as an efficient solid phase extraction sorbent for HPLC-PDA monitoring of parabens in personal care products.
33011479	3	78	theme	heterogeneous	603:615	arg1	texture					625:631	a heterogeneous, porous texture	601:631	a heterogeneous, porous texture	601:631	CS-Ni/Fe LDH composites with a heterogeneous, porous texture, and coral reef-like structure exhibit appealing extraction efficiency for the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions.
33011479	4	79	theme	parabens	919:926	arg1	extraction					905:914	solid phase extraction	893:914	solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen)	893:1024	The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen).
33011479	8	80	theme	prepared	1501:1508	arg1	composites					1510:1519	The prepared composites	1497:1519	The prepared composites	1497:1519	The prepared composites also displayed excellent performance with enhanced reusability/durability (n = 30 cycles) and reproducibility (n = 5).
33011479	7	81	theme	L-1	1478:1480	arg1	limits					1389:1394	limits	1389:1394	limits of detection (LOD)	1389:1413	The quantitation of MP, EP, PP, and BP was made at detection range (and limits of detection (LOD)) of 5-15 (9.8), 11-21 (16.2), 6-18 (12.4), and 10-20 (15.6) ng L-1, respectively.
33011479	7	81	theme	L-1	1478:1480	arg1	range					1378:1382	detection range	1368:1382	detection range	1368:1382	The quantitation of MP, EP, PP, and BP was made at detection range (and limits of detection (LOD)) of 5-15 (9.8), 11-21 (16.2), 6-18 (12.4), and 10-20 (15.6) ng L-1, respectively.
33011479	5	82	theme	good	1141:1144	arg1	precision					1146:1154	good precision	1141:1154	good precision (relative standard deviation (RSD) < 3%)	1141:1195	The LDH-SPE-HPLC method exhibited a wide linear range (e.g., 100-50,000 ng L-1), good linearity (R2 ≥ 0.999), and good precision (relative standard deviation (RSD) < 3%).
33011479	0	83	theme	efficient	57:65	arg1	extraction					79:88	an efficient solid phase extraction	54:88	an efficient solid phase extraction	54:88	Chitosan-Ni/Fe layered double hydroxide composites as an efficient solid phase extraction sorbent for HPLC-PDA monitoring of parabens in personal care products.
33011479	3	84	theme	enhanced	739:746	arg1	possibility					748:758	the enhanced possibility	735:758	the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions	735:825	CS-Ni/Fe LDH composites with a heterogeneous, porous texture, and coral reef-like structure exhibit appealing extraction efficiency for the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions.
33255098	8	0	theme	HAp	1310:1312	arg1	concentration					1293:1305	increased concentration	1283:1305	increased concentration of HAp	1283:1312	The SEM of the samples after cell culture revealed that these composites allowed a proliferation of the fibroblasts over and within the nanofiber network, and increased concentration of HAp levitated the excessive of apatite formation as well as increased cell growth.
33255098	1	1	theme	acetate-derived	298:312	arg1	threads					314:320	its acetate-derived threads	294:320	its acetate-derived threads	294:320	Cellulose nanofibers, which are troublesome to spin into fibers, can be easily fabricated by post-regeneration of its acetate-derived threads.
33255098	7	2	theme	embryo	1104:1109	arg1	fibroblasts					1111:1121	chicken embryo fibroblasts	1096:1121	chicken embryo fibroblasts	1096:1121	These nanofibers were cytocompatible, as resolved by MTT assay conducted on chicken embryo fibroblasts.
33255098	11	3	contain	possess	1754:1760	arg1	nanofibers					1743:1752	these nanofibers	1737:1752	these nanofibers	1737:1752	The results certify that these nanofibers possess enormous potential for soft and hard tissue engineering besides their antimicrobial properties.
33255098	11	3	contain	possess	1754:1760	arg2	potential					1771:1779	enormous potential	1762:1779	enormous potential for soft and hard tissue engineering besides their antimicrobial properties	1762:1855	The results certify that these nanofibers possess enormous potential for soft and hard tissue engineering besides their antimicrobial properties.
33255098	4	4	theme	cellulose	579:587	arg1	nanofibers					589:598	The resultant cellulose nanofibers	565:598	The resultant cellulose nanofibers previously loaded with hydroxyapatite (HAp)	565:642	The resultant cellulose nanofibers previously loaded with hydroxyapatite (HAp) were immobilized using silver (Ag) nanoparticles (NPs) by reduction of adsorbed Ag ions on using sodium borohydride.
33255098	1	5	theme	threads	314:320	arg1	post-regeneration					273:289	post-regeneration	273:289	post-regeneration of its acetate-derived threads	273:320	Cellulose nanofibers, which are troublesome to spin into fibers, can be easily fabricated by post-regeneration of its acetate-derived threads.
33255098	7	6	theme	chicken	1096:1102	arg1	fibroblasts					1111:1121	chicken embryo fibroblasts	1096:1121	chicken embryo fibroblasts	1096:1121	These nanofibers were cytocompatible, as resolved by MTT assay conducted on chicken embryo fibroblasts.
33255098	4	7	theme	resultant	569:577	arg1	nanofibers					589:598	The resultant cellulose nanofibers	565:598	The resultant cellulose nanofibers previously loaded with hydroxyapatite (HAp)	565:642	The resultant cellulose nanofibers previously loaded with hydroxyapatite (HAp) were immobilized using silver (Ag) nanoparticles (NPs) by reduction of adsorbed Ag ions on using sodium borohydride.
33255098	0	8	theme	silver	96:101	arg1	scaffold					134:141	a scaffold	132:141	a scaffold for tissue engineering applications	132:177	Regenerated cellulose nanofibers from cellulose acetate: Incorporating hydroxyapatite (HAp) and silver (Ag) nanoparticles (NPs), as a scaffold for tissue engineering applications.
33255098	0	8	theme	silver	96:101	arg1	NPs					123:125	NPs	123:125	NPs	123:125	Regenerated cellulose nanofibers from cellulose acetate: Incorporating hydroxyapatite (HAp) and silver (Ag) nanoparticles (NPs), as a scaffold for tissue engineering applications.
33255098	0	8	theme	silver	96:101	arg1	nanoparticles					108:120	silver (Ag) nanoparticles	96:120	silver (Ag) nanoparticles (NPs)	96:126	Regenerated cellulose nanofibers from cellulose acetate: Incorporating hydroxyapatite (HAp) and silver (Ag) nanoparticles (NPs), as a scaffold for tissue engineering applications.
33255098	0	8	theme	silver	96:101	arg1	hydroxyapatite					71:84	hydroxyapatite	71:84	hydroxyapatite (HAp)	71:90	Regenerated cellulose nanofibers from cellulose acetate: Incorporating hydroxyapatite (HAp) and silver (Ag) nanoparticles (NPs), as a scaffold for tissue engineering applications.
33255098	0	9	theme	engineering	154:164	arg1	applications					166:177	tissue engineering applications	147:177	tissue engineering applications	147:177	Regenerated cellulose nanofibers from cellulose acetate: Incorporating hydroxyapatite (HAp) and silver (Ag) nanoparticles (NPs), as a scaffold for tissue engineering applications.
33255098	4	10	theme	sodium	741:746	arg1	borohydride					748:758	sodium borohydride	741:758	sodium borohydride	741:758	The resultant cellulose nanofibers previously loaded with hydroxyapatite (HAp) were immobilized using silver (Ag) nanoparticles (NPs) by reduction of adsorbed Ag ions on using sodium borohydride.
33255098	4	11	theme	ions	727:730	arg1	reduction					702:710	reduction	702:710	reduction of adsorbed Ag ions	702:730	The resultant cellulose nanofibers previously loaded with hydroxyapatite (HAp) were immobilized using silver (Ag) nanoparticles (NPs) by reduction of adsorbed Ag ions on using sodium borohydride.
33255098	5	12	theme	Ag	899:900	arg1	in/on					906:910	Ag NPs in/on	899:910	Ag NPs in/on	899:910	These amalgamated nanofibers were characterized for SEM, EDX, TEM, FTIR, and hydrophilicity tests revealing the existence of both HAp and Ag NPs in/on the nanofiber scaffolds.
33255098	0	13	theme	tissue	147:152	arg1	applications					166:177	tissue engineering applications	147:177	tissue engineering applications	147:177	Regenerated cellulose nanofibers from cellulose acetate: Incorporating hydroxyapatite (HAp) and silver (Ag) nanoparticles (NPs), as a scaffold for tissue engineering applications.
33255098	5	14	theme	FTIR	828:831	arg1	tests					853:857	SEM, EDX, TEM, FTIR, and hydrophilicity tests	813:857	SEM, EDX, TEM, FTIR, and hydrophilicity tests	813:857	These amalgamated nanofibers were characterized for SEM, EDX, TEM, FTIR, and hydrophilicity tests revealing the existence of both HAp and Ag NPs in/on the nanofiber scaffolds.
33255098	8	15	theme	cell	1153:1156	arg1	culture					1158:1164	cell culture	1153:1164	cell culture	1153:1164	The SEM of the samples after cell culture revealed that these composites allowed a proliferation of the fibroblasts over and within the nanofiber network, and increased concentration of HAp levitated the excessive of apatite formation as well as increased cell growth.
33255098	8	16	theme	apatite	1341:1347	arg1	excessive					1328:1336	excessive	1328:1336	excessive	1328:1336	The SEM of the samples after cell culture revealed that these composites allowed a proliferation of the fibroblasts over and within the nanofiber network, and increased concentration of HAp levitated the excessive of apatite formation as well as increased cell growth.
33255098	4	17	theme	silver	667:672	arg1	NPs					694:696	NPs	694:696	NPs	694:696	The resultant cellulose nanofibers previously loaded with hydroxyapatite (HAp) were immobilized using silver (Ag) nanoparticles (NPs) by reduction of adsorbed Ag ions on using sodium borohydride.
33255098	4	17	theme	silver	667:672	arg1	nanoparticles					679:691	silver (Ag) nanoparticles	667:691	silver (Ag) nanoparticles (NPs)	667:697	The resultant cellulose nanofibers previously loaded with hydroxyapatite (HAp) were immobilized using silver (Ag) nanoparticles (NPs) by reduction of adsorbed Ag ions on using sodium borohydride.
33255098	0	18	from	acetate	48:54	arg1	nanofibers					22:31	cellulose nanofibers	12:31	cellulose nanofibers from cellulose acetate	12:54	Regenerated cellulose nanofibers from cellulose acetate: Incorporating hydroxyapatite (HAp) and silver (Ag) nanoparticles (NPs), as a scaffold for tissue engineering applications.
33255098	6	19	theme	nanofibers	969:978	arg1	de-acetylation					941:954	The de-acetylation	937:954	The de-acetylation of composite nanofibers	937:978	The de-acetylation of composite nanofibers resulted in spontaneous hydrophilicity.
33255098	3	20	theme	alkaline	518:525	arg1	de-acetylation					527:540	alkaline de-acetylation	518:540	alkaline de-acetylation of as-spun nanofibers	518:562	Herein, we regenerated acetate-free nanofibers by alkaline de-acetylation of as-spun nanofibers.
33255098	9	21	theme	nanofibers	1429:1438	arg1	activity					1411:1418	The antimicrobial activity	1393:1418	The antimicrobial activity of these nanofibers	1393:1438	The antimicrobial activity of these nanofibers was assessed on E. coli (BL21) and S. aureus, suggesting the potential of de-acetylated nanofibers to restrain bacterial growth.
33255098	0	22	theme	cellulose	12:20	arg1	nanofibers					22:31	cellulose nanofibers	12:31	cellulose nanofibers from cellulose acetate	12:54	Regenerated cellulose nanofibers from cellulose acetate: Incorporating hydroxyapatite (HAp) and silver (Ag) nanoparticles (NPs), as a scaffold for tissue engineering applications.
33255098	4	23	theme	Ag	724:725	arg1	ions					727:730	adsorbed Ag ions	715:730	adsorbed Ag ions	715:730	The resultant cellulose nanofibers previously loaded with hydroxyapatite (HAp) were immobilized using silver (Ag) nanoparticles (NPs) by reduction of adsorbed Ag ions on using sodium borohydride.
33255098	10	24	theme	degradation	1573:1583	arg1	study					1585:1589	The degradation study	1569:1589	The degradation study for 10, 30, and 60 days	1569:1613	The degradation study for 10, 30, and 60 days indicated degradation of the fibers much is faster in enzymes as compared to degradation in PBS.
33255098	7	25	theme	MTT	1073:1075	arg1	assay					1077:1081	MTT assay	1073:1081	MTT assay conducted on chicken embryo fibroblasts	1073:1121	These nanofibers were cytocompatible, as resolved by MTT assay conducted on chicken embryo fibroblasts.
33255098	4	26	theme	adsorbed	715:722	arg1	ions					727:730	adsorbed Ag ions	715:730	adsorbed Ag ions	715:730	The resultant cellulose nanofibers previously loaded with hydroxyapatite (HAp) were immobilized using silver (Ag) nanoparticles (NPs) by reduction of adsorbed Ag ions on using sodium borohydride.
33255098	5	27	theme	hydrophilicity	838:851	arg1	tests					853:857	SEM, EDX, TEM, FTIR, and hydrophilicity tests	813:857	SEM, EDX, TEM, FTIR, and hydrophilicity tests	813:857	These amalgamated nanofibers were characterized for SEM, EDX, TEM, FTIR, and hydrophilicity tests revealing the existence of both HAp and Ag NPs in/on the nanofiber scaffolds.
33255098	5	28	theme	SEM	813:815	arg1	tests					853:857	SEM, EDX, TEM, FTIR, and hydrophilicity tests	813:857	SEM, EDX, TEM, FTIR, and hydrophilicity tests	813:857	These amalgamated nanofibers were characterized for SEM, EDX, TEM, FTIR, and hydrophilicity tests revealing the existence of both HAp and Ag NPs in/on the nanofiber scaffolds.
33255098	8	29	theme	increased	1283:1291	arg1	concentration					1293:1305	increased concentration	1283:1305	increased concentration of HAp	1283:1312	The SEM of the samples after cell culture revealed that these composites allowed a proliferation of the fibroblasts over and within the nanofiber network, and increased concentration of HAp levitated the excessive of apatite formation as well as increased cell growth.
33255098	11	30	theme	antimicrobial	1832:1844	arg1	properties					1846:1855	their antimicrobial properties	1826:1855	their antimicrobial properties	1826:1855	The results certify that these nanofibers possess enormous potential for soft and hard tissue engineering besides their antimicrobial properties.
33255098	3	31	theme	as-spun	545:551	arg1	nanofibers					553:562	as-spun nanofibers	545:562	as-spun nanofibers	545:562	Herein, we regenerated acetate-free nanofibers by alkaline de-acetylation of as-spun nanofibers.
33255098	5	32	theme	EDX	818:820	arg1	tests					853:857	SEM, EDX, TEM, FTIR, and hydrophilicity tests	813:857	SEM, EDX, TEM, FTIR, and hydrophilicity tests	813:857	These amalgamated nanofibers were characterized for SEM, EDX, TEM, FTIR, and hydrophilicity tests revealing the existence of both HAp and Ag NPs in/on the nanofiber scaffolds.
33255098	5	33	theme	NPs	902:904	arg1	in/on					906:910	Ag NPs in/on	899:910	Ag NPs in/on	899:910	These amalgamated nanofibers were characterized for SEM, EDX, TEM, FTIR, and hydrophilicity tests revealing the existence of both HAp and Ag NPs in/on the nanofiber scaffolds.
33255098	9	34	theme	de-acetylated	1514:1526	arg1	nanofibers					1528:1537	de-acetylated nanofibers	1514:1537	de-acetylated nanofibers	1514:1537	The antimicrobial activity of these nanofibers was assessed on E. coli (BL21) and S. aureus, suggesting the potential of de-acetylated nanofibers to restrain bacterial growth.
33255098	3	35	theme	nanofibers	553:562	arg1	de-acetylation					527:540	alkaline de-acetylation	518:540	alkaline de-acetylation of as-spun nanofibers	518:562	Herein, we regenerated acetate-free nanofibers by alkaline de-acetylation of as-spun nanofibers.
33255098	2	36	theme	natural	338:344	arg1	polymer					346:352	a natural polymer	336:352	a natural polymer	336:352	Cellulose is a natural polymer; it enjoys better biocompatibility, cellular mimicking, and hydrophilic properties than its proportionate analog.
33255098	2	36	theme	natural	338:344	arg1	Cellulose					323:331	Cellulose	323:331	Cellulose	323:331	Cellulose is a natural polymer; it enjoys better biocompatibility, cellular mimicking, and hydrophilic properties than its proportionate analog.
33255098	2	37	theme	proportionate	446:458	arg1	analog					460:465	its proportionate analog	442:465	its proportionate analog	442:465	Cellulose is a natural polymer; it enjoys better biocompatibility, cellular mimicking, and hydrophilic properties than its proportionate analog.
33255098	5	38	theme	TEM	823:825	arg1	tests					853:857	SEM, EDX, TEM, FTIR, and hydrophilicity tests	813:857	SEM, EDX, TEM, FTIR, and hydrophilicity tests	813:857	These amalgamated nanofibers were characterized for SEM, EDX, TEM, FTIR, and hydrophilicity tests revealing the existence of both HAp and Ag NPs in/on the nanofiber scaffolds.
33255098	8	39	theme	cell	1380:1383	arg1	growth					1385:1390	increased cell growth	1370:1390	the excessive of apatite formation as well as increased cell growth	1324:1390	The SEM of the samples after cell culture revealed that these composites allowed a proliferation of the fibroblasts over and within the nanofiber network, and increased concentration of HAp levitated the excessive of apatite formation as well as increased cell growth.
33255098	1	40	link	acetate-derived	298:312	arg1	threads					314:320	its acetate-derived threads	294:320	its acetate-derived threads	294:320	Cellulose nanofibers, which are troublesome to spin into fibers, can be easily fabricated by post-regeneration of its acetate-derived threads.
33255098	1	41	theme	Cellulose	180:188	arg1	nanofibers					190:199	Cellulose nanofibers	180:199	Cellulose nanofibers	180:199	Cellulose nanofibers, which are troublesome to spin into fibers, can be easily fabricated by post-regeneration of its acetate-derived threads.
33255098	0	42	theme	cellulose	38:46	arg1	acetate					48:54	cellulose acetate	38:54	cellulose acetate	38:54	Regenerated cellulose nanofibers from cellulose acetate: Incorporating hydroxyapatite (HAp) and silver (Ag) nanoparticles (NPs), as a scaffold for tissue engineering applications.
33255098	5	43	theme	amalgamated	767:777	arg1	nanofibers					779:788	These amalgamated nanofibers	761:788	These amalgamated nanofibers	761:788	These amalgamated nanofibers were characterized for SEM, EDX, TEM, FTIR, and hydrophilicity tests revealing the existence of both HAp and Ag NPs in/on the nanofiber scaffolds.
33255098	6	44	theme	composite	959:967	arg1	nanofibers					969:978	composite nanofibers	959:978	composite nanofibers	959:978	The de-acetylation of composite nanofibers resulted in spontaneous hydrophilicity.
33255098	8	45	theme	increased	1370:1378	arg1	growth					1385:1390	increased cell growth	1370:1390	the excessive of apatite formation as well as increased cell growth	1324:1390	The SEM of the samples after cell culture revealed that these composites allowed a proliferation of the fibroblasts over and within the nanofiber network, and increased concentration of HAp levitated the excessive of apatite formation as well as increased cell growth.
33255098	8	46	theme	samples	1139:1145	arg1	SEM					1128:1130	The SEM	1124:1130	The SEM of the samples after cell culture	1124:1164	The SEM of the samples after cell culture revealed that these composites allowed a proliferation of the fibroblasts over and within the nanofiber network, and increased concentration of HAp levitated the excessive of apatite formation as well as increased cell growth.
33255098	5	47	dep	HAp	891:893	arg1	scaffolds					926:934	the nanofiber scaffolds	912:934	the nanofiber scaffolds	912:934	These amalgamated nanofibers were characterized for SEM, EDX, TEM, FTIR, and hydrophilicity tests revealing the existence of both HAp and Ag NPs in/on the nanofiber scaffolds.
33255098	11	48	theme	enormous	1762:1769	arg1	potential					1771:1779	enormous potential	1762:1779	enormous potential for soft and hard tissue engineering besides their antimicrobial properties	1762:1855	The results certify that these nanofibers possess enormous potential for soft and hard tissue engineering besides their antimicrobial properties.
33255098	8	49	theme	fibroblasts	1228:1238	arg1	proliferation					1207:1219	a proliferation	1205:1219	a proliferation of the fibroblasts	1205:1238	The SEM of the samples after cell culture revealed that these composites allowed a proliferation of the fibroblasts over and within the nanofiber network, and increased concentration of HAp levitated the excessive of apatite formation as well as increased cell growth.
33255098	11	50	dep	soft	1785:1788	arg1	engineering					1806:1816	tissue engineering	1799:1816	tissue engineering	1799:1816	The results certify that these nanofibers possess enormous potential for soft and hard tissue engineering besides their antimicrobial properties.
33255098	8	51	theme	nanofiber	1260:1268	arg1	network					1270:1276	the nanofiber network	1256:1276	the nanofiber network	1256:1276	The SEM of the samples after cell culture revealed that these composites allowed a proliferation of the fibroblasts over and within the nanofiber network, and increased concentration of HAp levitated the excessive of apatite formation as well as increased cell growth.
33255098	5	52	theme	HAp	891:893	arg1	existence					873:881	the existence	869:881	the existence of both HAp and Ag NPs in/on the nanofiber scaffolds	869:934	These amalgamated nanofibers were characterized for SEM, EDX, TEM, FTIR, and hydrophilicity tests revealing the existence of both HAp and Ag NPs in/on the nanofiber scaffolds.
33255098	5	53	theme	in/on	906:910	arg1	existence					873:881	the existence	869:881	the existence of both HAp and Ag NPs in/on the nanofiber scaffolds	869:934	These amalgamated nanofibers were characterized for SEM, EDX, TEM, FTIR, and hydrophilicity tests revealing the existence of both HAp and Ag NPs in/on the nanofiber scaffolds.
33255098	9	54	theme	nanofibers	1528:1537	arg1	potential					1501:1509	the potential	1497:1509	the potential of de-acetylated nanofibers to restrain bacterial growth	1497:1566	The antimicrobial activity of these nanofibers was assessed on E. coli (BL21) and S. aureus, suggesting the potential of de-acetylated nanofibers to restrain bacterial growth.
33255098	9	55	theme	antimicrobial	1397:1409	arg1	activity					1411:1418	The antimicrobial activity	1393:1418	The antimicrobial activity of these nanofibers	1393:1438	The antimicrobial activity of these nanofibers was assessed on E. coli (BL21) and S. aureus, suggesting the potential of de-acetylated nanofibers to restrain bacterial growth.
33255098	5	56	theme	nanofiber	916:924	arg1	scaffolds					926:934	the nanofiber scaffolds	912:934	the nanofiber scaffolds	912:934	These amalgamated nanofibers were characterized for SEM, EDX, TEM, FTIR, and hydrophilicity tests revealing the existence of both HAp and Ag NPs in/on the nanofiber scaffolds.
33255098	11	57	theme	tissue	1799:1804	arg1	engineering					1806:1816	tissue engineering	1799:1816	tissue engineering	1799:1816	The results certify that these nanofibers possess enormous potential for soft and hard tissue engineering besides their antimicrobial properties.
33255098	6	58	theme	spontaneous	992:1002	arg1	hydrophilicity					1004:1017	spontaneous hydrophilicity	992:1017	spontaneous hydrophilicity	992:1017	The de-acetylation of composite nanofibers resulted in spontaneous hydrophilicity.
33255098	8	59	theme	excessive	1328:1336	arg1	formation					1349:1357	the excessive of apatite formation	1324:1357	the excessive of apatite formation as well as increased cell growth	1324:1390	The SEM of the samples after cell culture revealed that these composites allowed a proliferation of the fibroblasts over and within the nanofiber network, and increased concentration of HAp levitated the excessive of apatite formation as well as increased cell growth.
33255098	10	60	theme	fibers	1644:1649	arg1	degradation					1625:1635	degradation	1625:1635	degradation of the fibers	1625:1649	The degradation study for 10, 30, and 60 days indicated degradation of the fibers much is faster in enzymes as compared to degradation in PBS.
33255098	3	61	theme	acetate-free	491:502	arg1	nanofibers					504:513	acetate-free nanofibers	491:513	acetate-free nanofibers	491:513	Herein, we regenerated acetate-free nanofibers by alkaline de-acetylation of as-spun nanofibers.
33255098	10	62	from	degradation	1692:1702	arg1	PBS					1707:1709	PBS	1707:1709	PBS	1707:1709	The degradation study for 10, 30, and 60 days indicated degradation of the fibers much is faster in enzymes as compared to degradation in PBS.
33255098	9	63	theme	bacterial	1551:1559	arg1	growth					1561:1566	bacterial growth	1551:1566	bacterial growth	1551:1566	The antimicrobial activity of these nanofibers was assessed on E. coli (BL21) and S. aureus, suggesting the potential of de-acetylated nanofibers to restrain bacterial growth.
33255098	2	64	theme	hydrophilic	414:424	arg1	properties					426:435	hydrophilic properties	414:435	hydrophilic properties	414:435	Cellulose is a natural polymer; it enjoys better biocompatibility, cellular mimicking, and hydrophilic properties than its proportionate analog.
33999089	8	0	theme	U	1349:1349	arg1	mL-1					1351:1354	1.0-36 U mL-1	1342:1354	1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1	1342:1395	Under the optimized conditions, the amount of water collected within 5 min showed a linear relationship with the HAase concentration in a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1.
33999089	7	1	theme	membrane	1113:1120	arg1	composite					1096:1104	The composite	1092:1104	The composite of the membrane	1092:1120	The composite of the membrane, and the reaction conditions after the addition of HAase were optimized.
33999089	7	1	theme	membrane	1113:1120	arg1	membrane					1113:1120	the membrane	1109:1120	the membrane	1109:1120	The composite of the membrane, and the reaction conditions after the addition of HAase were optimized.
33999089	7	1	theme	membrane	1113:1120	arg1	conditions					1140:1149	the reaction conditions	1127:1149	the reaction conditions after the addition of HAase	1127:1177	The composite of the membrane, and the reaction conditions after the addition of HAase were optimized.
33999089	0	2	theme	permeability	81:92	arg1	enhancing					64:72	the target-trigger enhancing	45:72	the target-trigger enhancing of the permeability of a membrane	45:106	Convenient hyaluronidase biosensors based on the target-trigger enhancing of the permeability of a membrane using an electronic balance as a readout.
33999089	9	3	theme	satisfactory	1475:1486	arg1	results					1488:1494	satisfactory results	1475:1494	satisfactory results	1475:1494	The proposed method has been applied to detect HAase in vitreous samples with satisfactory results.
33999089	0	4	theme	membrane	99:106	arg1	permeability					81:92	the permeability	77:92	the permeability of a membrane	77:106	Convenient hyaluronidase biosensors based on the target-trigger enhancing of the permeability of a membrane using an electronic balance as a readout.
33999089	1	5	theme	HAase	311:315	arg1	detection					317:325	HAase detection	311:325	HAase detection	311:325	The change in hyaluronidase (HAase) is related to specific changes in the structure of vitreous, and it is necessary to develop simple but sensitive methods for HAase detection.
33999089	1	6	from	change	154:159	arg1	HAase					179:183	HAase	179:183	HAase	179:183	The change in hyaluronidase (HAase) is related to specific changes in the structure of vitreous, and it is necessary to develop simple but sensitive methods for HAase detection.
33999089	1	6	from	change	154:159	arg1	hyaluronidase					164:176	hyaluronidase	164:176	hyaluronidase (HAase)	164:184	The change in hyaluronidase (HAase) is related to specific changes in the structure of vitreous, and it is necessary to develop simple but sensitive methods for HAase detection.
33999089	6	7	theme	composite	1050:1058	arg1	membrane					1060:1067	the HA-PEI-MCEM composite membrane	1034:1067	the HA-PEI-MCEM composite membrane	1034:1067	Therefore, the amount of water passing through the HA-PEI-MCEM composite membrane increased accordingly.
33999089	1	8	theme	vitreous	237:244	arg1	structure					224:232	the structure	220:232	the structure of vitreous	220:244	The change in hyaluronidase (HAase) is related to specific changes in the structure of vitreous, and it is necessary to develop simple but sensitive methods for HAase detection.
33999089	8	9	theme	optimized	1205:1213	arg1	conditions					1215:1224	the optimized conditions	1201:1224	the optimized conditions	1201:1224	Under the optimized conditions, the amount of water collected within 5 min showed a linear relationship with the HAase concentration in a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1.
33999089	6	10	theme	HA-PEI-MCEM	1038:1048	arg1	membrane					1060:1067	the HA-PEI-MCEM composite membrane	1034:1067	the HA-PEI-MCEM composite membrane	1034:1067	Therefore, the amount of water passing through the HA-PEI-MCEM composite membrane increased accordingly.
33999089	8	11	with	mL-1	1351:1354	arg1	limit					1363:1367	a limit	1361:1367	a limit of detection of 0.35 U mL-1	1361:1395	Under the optimized conditions, the amount of water collected within 5 min showed a linear relationship with the HAase concentration in a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1.
33999089	2	12	theme	thin	344:347	arg1	film					349:352	a thin film	342:352	a thin film fabricated from a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel	342:424	In this work, a thin film fabricated from a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel has been covered on a mixed cellulose microporous membrane (MCEM) to form a HA-PEI-MCEM firstly and it was then applied in a filtration system.
33999089	4	13	theme	water	849:853	arg1	water					849:853	water	849:853	water	849:853	The low permeability of the HA-PEI-MCEM allows a small amount of water to be drained.
33999089	4	13	theme	water	849:853	arg1	amount					839:844	a small amount	831:844	a small amount of water	831:853	The low permeability of the HA-PEI-MCEM allows a small amount of water to be drained.
33999089	5	14	theme	membrane	967:974	arg1	permeability					947:958	the permeability	943:958	the permeability of the membrane	943:974	But after the addition of HAase, which can hydrolyze HA in the hydrogel, the permeability of the membrane increased.
33999089	4	15	theme	small	833:837	arg1	water					849:853	water	849:853	water	849:853	The low permeability of the HA-PEI-MCEM allows a small amount of water to be drained.
33999089	4	15	theme	small	833:837	arg1	amount					839:844	a small amount	831:844	a small amount of water	831:853	The low permeability of the HA-PEI-MCEM allows a small amount of water to be drained.
33999089	8	16	theme	detection	1372:1380	arg1	limit					1363:1367	a limit	1361:1367	a limit of detection of 0.35 U mL-1	1361:1395	Under the optimized conditions, the amount of water collected within 5 min showed a linear relationship with the HAase concentration in a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1.
33999089	4	17	theme	low	788:790	arg1	permeability					792:803	The low permeability	784:803	The low permeability of the HA-PEI-MCEM	784:822	The low permeability of the HA-PEI-MCEM allows a small amount of water to be drained.
33999089	3	18	theme	filter	594:599	arg1	membrane					601:608	the filter membrane	590:608	the filter membrane	590:608	The permeability of the filter membrane greatly affects the amount of water passing through within a certain time and the water can be collected and quantitatively measured with a simple electronic balance easily.
33999089	0	19	theme	Convenient	0:9	arg1	hyaluronidase					11:23	Convenient hyaluronidase	0:23	Convenient hyaluronidase	0:23	Convenient hyaluronidase biosensors based on the target-trigger enhancing of the permeability of a membrane using an electronic balance as a readout.
33999089	8	20	theme	U	1390:1390	arg1	mL-1					1392:1395	0.35 U mL-1	1385:1395	0.35 U mL-1	1385:1395	Under the optimized conditions, the amount of water collected within 5 min showed a linear relationship with the HAase concentration in a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1.
33999089	6	21	theme	water	1012:1016	arg1	water					1012:1016	water	1012:1016	water passing through the HA-PEI-MCEM composite membrane	1012:1067	Therefore, the amount of water passing through the HA-PEI-MCEM composite membrane increased accordingly.
33999089	6	21	theme	water	1012:1016	arg1	amount					1002:1007	the amount	998:1007	the amount of water passing through the HA-PEI-MCEM composite membrane	998:1067	Therefore, the amount of water passing through the HA-PEI-MCEM composite membrane increased accordingly.
33999089	2	22	theme	acid	383:386	arg1	hydrogel					417:424	a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel	370:424	a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel	370:424	In this work, a thin film fabricated from a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel has been covered on a mixed cellulose microporous membrane (MCEM) to form a HA-PEI-MCEM firstly and it was then applied in a filtration system.
33999089	8	23	theme	mL-1	1351:1354	arg1	range					1333:1337	a range	1331:1337	a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1	1331:1395	Under the optimized conditions, the amount of water collected within 5 min showed a linear relationship with the HAase concentration in a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1.
33999089	7	24	theme	HAase	1173:1177	arg1	addition					1161:1168	the addition	1157:1168	the addition of HAase	1157:1177	The composite of the membrane, and the reaction conditions after the addition of HAase were optimized.
33999089	2	25	theme	hyaluronic	372:381	arg1	HA					389:390	HA	389:390	HA	389:390	In this work, a thin film fabricated from a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel has been covered on a mixed cellulose microporous membrane (MCEM) to form a HA-PEI-MCEM firstly and it was then applied in a filtration system.
33999089	2	25	theme	hyaluronic	372:381	arg1	acid					383:386	a hyaluronic acid	370:386	a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel	370:424	In this work, a thin film fabricated from a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel has been covered on a mixed cellulose microporous membrane (MCEM) to form a HA-PEI-MCEM firstly and it was then applied in a filtration system.
33999089	3	26	theme	water	640:644	arg1	water					640:644	water	640:644	water passing through within a certain time	640:682	The permeability of the filter membrane greatly affects the amount of water passing through within a certain time and the water can be collected and quantitatively measured with a simple electronic balance easily.
33999089	3	26	theme	water	640:644	arg1	amount					630:635	the amount	626:635	the amount of water passing through within a certain time	626:682	The permeability of the filter membrane greatly affects the amount of water passing through within a certain time and the water can be collected and quantitatively measured with a simple electronic balance easily.
33999089	0	27	theme	electronic	117:126	arg1	balance					128:134	an electronic balance	114:134	an electronic balance	114:134	Convenient hyaluronidase biosensors based on the target-trigger enhancing of the permeability of a membrane using an electronic balance as a readout.
33999089	0	27	theme	electronic	117:126	arg1	readout					141:147	a readout	139:147	a readout	139:147	Convenient hyaluronidase biosensors based on the target-trigger enhancing of the permeability of a membrane using an electronic balance as a readout.
33999089	8	28	theme	HAase	1308:1312	arg1	concentration					1314:1326	the HAase concentration	1304:1326	the HAase concentration in a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1	1304:1395	Under the optimized conditions, the amount of water collected within 5 min showed a linear relationship with the HAase concentration in a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1.
33999089	8	29	theme	water	1241:1245	arg1	water					1241:1245	water	1241:1245	water collected within 5 min	1241:1268	Under the optimized conditions, the amount of water collected within 5 min showed a linear relationship with the HAase concentration in a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1.
33999089	8	29	theme	water	1241:1245	arg1	amount					1231:1236	the amount	1227:1236	the amount of water collected within 5 min	1227:1268	Under the optimized conditions, the amount of water collected within 5 min showed a linear relationship with the HAase concentration in a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1.
33999089	2	30	theme	PEI	412:414	arg1	hydrogel					417:424	a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel	370:424	a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel	370:424	In this work, a thin film fabricated from a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel has been covered on a mixed cellulose microporous membrane (MCEM) to form a HA-PEI-MCEM firstly and it was then applied in a filtration system.
33999089	8	31	theme	mL-1	1392:1395	arg1	detection					1372:1380	detection	1372:1380	detection of 0.35 U mL-1	1372:1395	Under the optimized conditions, the amount of water collected within 5 min showed a linear relationship with the HAase concentration in a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1.
33999089	2	32	theme	microporous	464:474	arg1	membrane					476:483	a mixed cellulose microporous membrane	446:483	a mixed cellulose microporous membrane (MCEM)	446:490	In this work, a thin film fabricated from a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel has been covered on a mixed cellulose microporous membrane (MCEM) to form a HA-PEI-MCEM firstly and it was then applied in a filtration system.
33999089	2	32	theme	microporous	464:474	arg1	MCEM					486:489	MCEM	486:489	MCEM	486:489	In this work, a thin film fabricated from a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel has been covered on a mixed cellulose microporous membrane (MCEM) to form a HA-PEI-MCEM firstly and it was then applied in a filtration system.
33999089	5	33	theme	HAase	896:900	arg1	addition					884:891	the addition	880:891	the addition	880:891	But after the addition of HAase, which can hydrolyze HA in the hydrogel, the permeability of the membrane increased.
33999089	2	34	theme	cellulose	454:462	arg1	membrane					476:483	a mixed cellulose microporous membrane	446:483	a mixed cellulose microporous membrane (MCEM)	446:490	In this work, a thin film fabricated from a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel has been covered on a mixed cellulose microporous membrane (MCEM) to form a HA-PEI-MCEM firstly and it was then applied in a filtration system.
33999089	2	34	theme	cellulose	454:462	arg1	MCEM					486:489	MCEM	486:489	MCEM	486:489	In this work, a thin film fabricated from a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel has been covered on a mixed cellulose microporous membrane (MCEM) to form a HA-PEI-MCEM firstly and it was then applied in a filtration system.
33999089	2	35	theme	-polyethyleneimine	392:409	arg1	hydrogel					417:424	a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel	370:424	a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel	370:424	In this work, a thin film fabricated from a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel has been covered on a mixed cellulose microporous membrane (MCEM) to form a HA-PEI-MCEM firstly and it was then applied in a filtration system.
33999089	2	36	theme	mixed	448:452	arg1	membrane					476:483	a mixed cellulose microporous membrane	446:483	a mixed cellulose microporous membrane (MCEM)	446:490	In this work, a thin film fabricated from a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel has been covered on a mixed cellulose microporous membrane (MCEM) to form a HA-PEI-MCEM firstly and it was then applied in a filtration system.
33999089	2	36	theme	mixed	448:452	arg1	MCEM					486:489	MCEM	486:489	MCEM	486:489	In this work, a thin film fabricated from a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel has been covered on a mixed cellulose microporous membrane (MCEM) to form a HA-PEI-MCEM firstly and it was then applied in a filtration system.
33999089	9	37	with	samples	1462:1468	arg1	results					1488:1494	satisfactory results	1475:1494	satisfactory results	1475:1494	The proposed method has been applied to detect HAase in vitreous samples with satisfactory results.
33999089	0	38	theme	target-trigger	49:62	arg1	enhancing					64:72	the target-trigger enhancing	45:72	the target-trigger enhancing of the permeability of a membrane	45:106	Convenient hyaluronidase biosensors based on the target-trigger enhancing of the permeability of a membrane using an electronic balance as a readout.
33999089	1	39	theme	simple	278:283	arg1	methods					299:305	simple but sensitive methods	278:305	simple but sensitive methods for HAase detection	278:325	The change in hyaluronidase (HAase) is related to specific changes in the structure of vitreous, and it is necessary to develop simple but sensitive methods for HAase detection.
33999089	3	40	theme	membrane	601:608	arg1	permeability					574:585	The permeability	570:585	The permeability of the filter membrane	570:608	The permeability of the filter membrane greatly affects the amount of water passing through within a certain time and the water can be collected and quantitatively measured with a simple electronic balance easily.
33999089	9	41	theme	proposed	1401:1408	arg1	method					1410:1415	The proposed method	1397:1415	The proposed method	1397:1415	The proposed method has been applied to detect HAase in vitreous samples with satisfactory results.
33999089	3	42	theme	simple	750:755	arg1	balance					768:774	a simple electronic balance	748:774	a simple electronic balance	748:774	The permeability of the filter membrane greatly affects the amount of water passing through within a certain time and the water can be collected and quantitatively measured with a simple electronic balance easily.
33999089	3	43	theme	certain	671:677	arg1	time					679:682	a certain time	669:682	a certain time	669:682	The permeability of the filter membrane greatly affects the amount of water passing through within a certain time and the water can be collected and quantitatively measured with a simple electronic balance easily.
33999089	8	44	theme	linear	1279:1284	arg1	relationship					1286:1297	a linear relationship	1277:1297	a linear relationship with the HAase concentration in a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1	1277:1395	Under the optimized conditions, the amount of water collected within 5 min showed a linear relationship with the HAase concentration in a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1.
33999089	2	45	theme	filtration	551:560	arg1	system					562:567	a filtration system	549:567	a filtration system	549:567	In this work, a thin film fabricated from a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel has been covered on a mixed cellulose microporous membrane (MCEM) to form a HA-PEI-MCEM firstly and it was then applied in a filtration system.
33999089	7	46	theme	reaction	1131:1138	arg1	conditions					1140:1149	the reaction conditions	1127:1149	the reaction conditions after the addition of HAase	1127:1177	The composite of the membrane, and the reaction conditions after the addition of HAase were optimized.
33999089	3	47	theme	electronic	757:766	arg1	balance					768:774	a simple electronic balance	748:774	a simple electronic balance	748:774	The permeability of the filter membrane greatly affects the amount of water passing through within a certain time and the water can be collected and quantitatively measured with a simple electronic balance easily.
33999089	1	48	theme	specific	200:207	arg1	changes					209:215	specific changes	200:215	specific changes in the structure of vitreous	200:244	The change in hyaluronidase (HAase) is related to specific changes in the structure of vitreous, and it is necessary to develop simple but sensitive methods for HAase detection.
33999089	1	49	from	changes	209:215	arg1	structure					224:232	the structure	220:232	the structure of vitreous	220:244	The change in hyaluronidase (HAase) is related to specific changes in the structure of vitreous, and it is necessary to develop simple but sensitive methods for HAase detection.
33999089	0	50	dep	biosensors	25:34	arg1	based					36:40	based	36:40	biosensors based on the target-trigger enhancing of the permeability of a membrane using an electronic balance as a readout	25:147	Convenient hyaluronidase biosensors based on the target-trigger enhancing of the permeability of a membrane using an electronic balance as a readout.
33999089	4	51	theme	HA-PEI-MCEM	812:822	arg1	permeability					792:803	The low permeability	784:803	The low permeability of the HA-PEI-MCEM	784:822	The low permeability of the HA-PEI-MCEM allows a small amount of water to be drained.
33999089	9	52	theme	vitreous	1453:1460	arg1	samples					1462:1468	vitreous samples	1453:1468	vitreous samples with satisfactory results	1453:1494	The proposed method has been applied to detect HAase in vitreous samples with satisfactory results.
33999089	8	53	from	concentration	1314:1326	arg1	range					1333:1337	a range	1331:1337	a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1	1331:1395	Under the optimized conditions, the amount of water collected within 5 min showed a linear relationship with the HAase concentration in a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1.
33999089	1	54	theme	sensitive	289:297	arg1	methods					299:305	simple but sensitive methods	278:305	simple but sensitive methods for HAase detection	278:325	The change in hyaluronidase (HAase) is related to specific changes in the structure of vitreous, and it is necessary to develop simple but sensitive methods for HAase detection.
33999089	8	55	with	relationship	1286:1297	arg1	concentration					1314:1326	the HAase concentration	1304:1326	the HAase concentration in a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1	1304:1395	Under the optimized conditions, the amount of water collected within 5 min showed a linear relationship with the HAase concentration in a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1.
34427087	12	0	theme	12AMOR1	1456:1462	arg1	enzyme					1469:1474	12AMOR1 GtfC enzyme	1456:1474	12AMOR1 GtfC enzyme	1456:1474	12AMOR1 GtfC enzyme showed clear antistaling effects in bread bakery products.
34427087	9	1	theme	maltogenic	1207:1216	arg1	α-amylase					1218:1226	a novel maltogenic α-amylase	1199:1226	a novel maltogenic α-amylase	1199:1226	In fact, this GtfC represents a novel maltogenic α-amylase.
34427087	5	2	theme	GtfC	604:607	arg1	enzymes					609:615	related GtfC enzymes	596:615	related GtfC enzymes	596:615	Database searches showed that related GtfC enzymes occur in Gram-positive bacteria of the genera Exiguobacterium, Bacillus, and Geobacillus, adapted to growth at more extreme temperatures.
34427087	12	3	theme	bakery	1518:1523	arg1	products					1525:1532	bread bakery products	1512:1532	bread bakery products	1512:1532	12AMOR1 GtfC enzyme showed clear antistaling effects in bread bakery products.
34427087	5	4	dep	Exiguobacterium	663:677	arg1	Bacillus					680:687	the genera Exiguobacterium, Bacillus, and Geobacillus, adapted to growth at more extreme temperatures	652:752	Bacillus	680:687	Database searches showed that related GtfC enzymes occur in Gram-positive bacteria of the genera Exiguobacterium, Bacillus, and Geobacillus, adapted to growth at more extreme temperatures.
34427087	10	5	theme	α-glucan	1288:1295	arg1	products					1297:1304	its starch-derived α-glucan products	1269:1304	its starch-derived α-glucan products	1269:1304	Detailed structural characterization of its starch-derived α-glucan products revealed that it yielded a unique polymer with alternating (α1 → 6)/(α1 → 4)-linked glucose units but without branches.
34427087	3	6	theme	linear	459:464	arg1	α-glucans					478:486	linear or branched α-glucans	459:486	linear or branched α-glucans	459:486	In previous work, we have characterized various 4,6- and 4,3-α-glucanotransferases of the glycosyl hydrolase (GH) family 70 (subfamily GtfB), synthesizing linear or branched α-glucans.
34427087	2	7	theme	α-glucanotransferase	214:233	arg1	enzymes					235:241	Starch-acting α-glucanotransferase enzymes	200:241	Starch-acting α-glucanotransferase enzymes	200:241	Starch-acting α-glucanotransferase enzymes are of great interest for applications in the food industry.
34427087	8	8	theme	thermostable	979:990	arg1	4,6-α-glucanotransferase					992:1015	This thermostable 4,6-α-glucanotransferase	974:1015	This thermostable 4,6-α-glucanotransferase	974:1015	This thermostable 4,6-α-glucanotransferase has a novel product specificity, cleaving off predominantly maltose units from amylose, attaching them with an (α1 → 6)-linkage to acceptor substrates.
34427087	10	9	theme	unique	1333:1338	arg1	polymer					1340:1346	a unique polymer	1331:1346	a unique polymer with alternating (α1 → 6)/(α1 → 4)-linked glucose units but without branches	1331:1423	Detailed structural characterization of its starch-derived α-glucan products revealed that it yielded a unique polymer with alternating (α1 → 6)/(α1 → 4)-linked glucose units but without branches.
34427087	10	10	dep	-linked	1382:1388	arg1	→					1378:1378	α1 → 4	1375:1380	α1 → 4	1375:1380	Detailed structural characterization of its starch-derived α-glucan products revealed that it yielded a unique polymer with alternating (α1 → 6)/(α1 → 4)-linked glucose units but without branches.
34427087	10	10	dep	-linked	1382:1388	arg1	→					1369:1369	α1 → 6)/	1366:1373	α1 → 6)/	1366:1373	Detailed structural characterization of its starch-derived α-glucan products revealed that it yielded a unique polymer with alternating (α1 → 6)/(α1 → 4)-linked glucose units but without branches.
34427087	1	11	theme	Novel	52:56	arg1	Type					71:74	a Novel Thermostable Type	50:74	a Novel Thermostable Type of GH70 4,6-α-Glucanotransferase That Synthesizes a Linear Alternating (α1 → 6)/(α1 → 4) α-Glucan and Delays Bread Staling	50:197	12AMOR1 Represents a Novel Thermostable Type of GH70 4,6-α-Glucanotransferase That Synthesizes a Linear Alternating (α1 → 6)/(α1 → 4) α-Glucan and Delays Bread Staling.
34427087	7	12	theme	melting	883:889	arg1	temperature					891:901	a melting temperature	881:901	a melting temperature of 68 °C	881:910	12AMOR1 GtfC enzyme, with an optimal reaction temperature of 60 °C and a melting temperature of 68 °C, allowing starch conversions at relatively high temperatures.
34427087	2	13	theme	great	250:254	arg1	interest					256:263	great interest	250:263	great interest	250:263	Starch-acting α-glucanotransferase enzymes are of great interest for applications in the food industry.
34427087	7	14	theme	12AMOR1	810:816	arg1	enzyme					823:828	12AMOR1 GtfC enzyme	810:828	12AMOR1 GtfC enzyme	810:828	12AMOR1 GtfC enzyme, with an optimal reaction temperature of 60 °C and a melting temperature of 68 °C, allowing starch conversions at relatively high temperatures.
34427087	1	15	theme	Alternating	135:145	arg1	Linear					128:133	a Linear Alternating	126:145	a Linear Alternating (α1 → 6)/	126:155	12AMOR1 Represents a Novel Thermostable Type of GH70 4,6-α-Glucanotransferase That Synthesizes a Linear Alternating (α1 → 6)/(α1 → 4) α-Glucan and Delays Bread Staling.
34427087	1	15	theme	Alternating	135:145	arg1	→					151:151	α1 → 6	148:153	α1 → 6	148:153	12AMOR1 Represents a Novel Thermostable Type of GH70 4,6-α-Glucanotransferase That Synthesizes a Linear Alternating (α1 → 6)/(α1 → 4) α-Glucan and Delays Bread Staling.
34427087	3	16	theme	previous	307:314	arg1	work					316:319	previous work	307:319	previous work	307:319	In previous work, we have characterized various 4,6- and 4,3-α-glucanotransferases of the glycosyl hydrolase (GH) family 70 (subfamily GtfB), synthesizing linear or branched α-glucans.
34427087	5	17	theme	Database	566:573	arg1	searches					575:582	Database searches	566:582	Database searches	566:582	Database searches showed that related GtfC enzymes occur in Gram-positive bacteria of the genera Exiguobacterium, Bacillus, and Geobacillus, adapted to growth at more extreme temperatures.
34427087	1	18	theme	Thermostable	58:69	arg1	Type					71:74	a Novel Thermostable Type	50:74	a Novel Thermostable Type of GH70 4,6-α-Glucanotransferase That Synthesizes a Linear Alternating (α1 → 6)/(α1 → 4) α-Glucan and Delays Bread Staling	50:197	12AMOR1 Represents a Novel Thermostable Type of GH70 4,6-α-Glucanotransferase That Synthesizes a Linear Alternating (α1 → 6)/(α1 → 4) α-Glucan and Delays Bread Staling.
34427087	7	19	theme	GtfC	818:821	arg1	enzyme					823:828	12AMOR1 GtfC enzyme	810:828	12AMOR1 GtfC enzyme	810:828	12AMOR1 GtfC enzyme, with an optimal reaction temperature of 60 °C and a melting temperature of 68 °C, allowing starch conversions at relatively high temperatures.
34427087	8	20	dep	units	1085:1089	arg1	amylose					1096:1102	amylose	1096:1102	amylose	1096:1102	This thermostable 4,6-α-glucanotransferase has a novel product specificity, cleaving off predominantly maltose units from amylose, attaching them with an (α1 → 6)-linkage to acceptor substrates.
34427087	12	21	theme	antistaling	1489:1499	arg1	effects					1501:1507	clear antistaling effects	1483:1507	clear antistaling effects	1483:1507	12AMOR1 GtfC enzyme showed clear antistaling effects in bread bakery products.
34427087	10	22	theme	-linked	1382:1388	arg1	units					1398:1402	alternating (α1 → 6)/(α1 → 4)-linked glucose units	1353:1402	alternating (α1 → 6)/(α1 → 4)-linked glucose units	1353:1402	Detailed structural characterization of its starch-derived α-glucan products revealed that it yielded a unique polymer with alternating (α1 → 6)/(α1 → 4)-linked glucose units but without branches.
34427087	3	23	theme	GH	414:415	arg1	family					418:423	the glycosyl hydrolase (GH) family 70	390:426	the glycosyl hydrolase (GH) family 70 (subfamily GtfB)	390:443	In previous work, we have characterized various 4,6- and 4,3-α-glucanotransferases of the glycosyl hydrolase (GH) family 70 (subfamily GtfB), synthesizing linear or branched α-glucans.
34427087	3	23	theme	GH	414:415	arg1	GtfB					439:442	subfamily GtfB	429:442	subfamily GtfB	429:442	In previous work, we have characterized various 4,6- and 4,3-α-glucanotransferases of the glycosyl hydrolase (GH) family 70 (subfamily GtfB), synthesizing linear or branched α-glucans.
34427087	3	24	theme	various	344:350	arg1	4,6-					352:355	various 4,6-	344:355	various 4,6-	344:355	In previous work, we have characterized various 4,6- and 4,3-α-glucanotransferases of the glycosyl hydrolase (GH) family 70 (subfamily GtfB), synthesizing linear or branched α-glucans.
34427087	10	25	with	polymer	1340:1346	arg1	units					1398:1402	alternating (α1 → 6)/(α1 → 4)-linked glucose units	1353:1402	alternating (α1 → 6)/(α1 → 4)-linked glucose units	1353:1402	Detailed structural characterization of its starch-derived α-glucan products revealed that it yielded a unique polymer with alternating (α1 → 6)/(α1 → 4)-linked glucose units but without branches.
34427087	8	26	theme	novel	1023:1027	arg1	specificity					1037:1047	a novel product specificity	1021:1047	a novel product specificity	1021:1047	This thermostable 4,6-α-glucanotransferase has a novel product specificity, cleaving off predominantly maltose units from amylose, attaching them with an (α1 → 6)-linkage to acceptor substrates.
34427087	12	27	theme	bread	1512:1516	arg1	products					1525:1532	bread bakery products	1512:1532	bread bakery products	1512:1532	12AMOR1 GtfC enzyme showed clear antistaling effects in bread bakery products.
34427087	3	28	theme	glycosyl	394:401	arg1	family					418:423	the glycosyl hydrolase (GH) family 70	390:426	the glycosyl hydrolase (GH) family 70 (subfamily GtfB)	390:443	In previous work, we have characterized various 4,6- and 4,3-α-glucanotransferases of the glycosyl hydrolase (GH) family 70 (subfamily GtfB), synthesizing linear or branched α-glucans.
34427087	3	28	theme	glycosyl	394:401	arg1	GtfB					439:442	subfamily GtfB	429:442	subfamily GtfB	429:442	In previous work, we have characterized various 4,6- and 4,3-α-glucanotransferases of the glycosyl hydrolase (GH) family 70 (subfamily GtfB), synthesizing linear or branched α-glucans.
34427087	8	29	theme	α1	1129:1130	arg1	→					1132:1132	α1 → 6	1129:1134	α1 → 6	1129:1134	This thermostable 4,6-α-glucanotransferase has a novel product specificity, cleaving off predominantly maltose units from amylose, attaching them with an (α1 → 6)-linkage to acceptor substrates.
34427087	8	29	theme	α1	1129:1130	arg1	-linkage					1136:1143	an (α1 → 6)-linkage	1125:1143	an (α1 → 6)-linkage to acceptor substrates	1125:1166	This thermostable 4,6-α-glucanotransferase has a novel product specificity, cleaving off predominantly maltose units from amylose, attaching them with an (α1 → 6)-linkage to acceptor substrates.
34427087	4	30	theme	GtfB	499:502	arg1	enzymes					504:510	GtfB enzymes	499:510	GtfB enzymes	499:510	Thus far, GtfB enzymes have only been identified in mesophilic Lactobacilli.
34427087	5	31	theme	related	596:602	arg1	enzymes					609:615	related GtfC enzymes	596:615	related GtfC enzymes	596:615	Database searches showed that related GtfC enzymes occur in Gram-positive bacteria of the genera Exiguobacterium, Bacillus, and Geobacillus, adapted to growth at more extreme temperatures.
34427087	1	32	theme	GH70	79:82	arg1	4,6-α-Glucanotransferase					84:107	GH70 4,6-α-Glucanotransferase	79:107	GH70 4,6-α-Glucanotransferase	79:107	12AMOR1 Represents a Novel Thermostable Type of GH70 4,6-α-Glucanotransferase That Synthesizes a Linear Alternating (α1 → 6)/(α1 → 4) α-Glucan and Delays Bread Staling.
34427087	3	33	theme	hydrolase	403:411	arg1	family					418:423	the glycosyl hydrolase (GH) family 70	390:426	the glycosyl hydrolase (GH) family 70 (subfamily GtfB)	390:443	In previous work, we have characterized various 4,6- and 4,3-α-glucanotransferases of the glycosyl hydrolase (GH) family 70 (subfamily GtfB), synthesizing linear or branched α-glucans.
34427087	3	33	theme	hydrolase	403:411	arg1	GtfB					439:442	subfamily GtfB	429:442	subfamily GtfB	429:442	In previous work, we have characterized various 4,6- and 4,3-α-glucanotransferases of the glycosyl hydrolase (GH) family 70 (subfamily GtfB), synthesizing linear or branched α-glucans.
34427087	0	34	theme	Geobacillus	15:25	arg1	sp					27:28	Geobacillus sp	15:28	Geobacillus sp	15:28	GtfC Enzyme of Geobacillus sp.
34427087	5	35	theme	genera	656:661	arg1	Exiguobacterium					663:677	the genera Exiguobacterium	652:677	the genera Exiguobacterium	652:677	Database searches showed that related GtfC enzymes occur in Gram-positive bacteria of the genera Exiguobacterium, Bacillus, and Geobacillus, adapted to growth at more extreme temperatures.
34427087	7	36	theme	°C	874:875	arg1	temperature					891:901	a melting temperature	881:901	a melting temperature of 68 °C	881:910	12AMOR1 GtfC enzyme, with an optimal reaction temperature of 60 °C and a melting temperature of 68 °C, allowing starch conversions at relatively high temperatures.
34427087	7	36	theme	°C	874:875	arg1	temperature					856:866	an optimal reaction temperature	836:866	an optimal reaction temperature of 60 °C	836:875	12AMOR1 GtfC enzyme, with an optimal reaction temperature of 60 °C and a melting temperature of 68 °C, allowing starch conversions at relatively high temperatures.
34427087	1	37	theme	4,6-α-Glucanotransferase	84:107	arg1	Type					71:74	a Novel Thermostable Type	50:74	a Novel Thermostable Type of GH70 4,6-α-Glucanotransferase That Synthesizes a Linear Alternating (α1 → 6)/(α1 → 4) α-Glucan and Delays Bread Staling	50:197	12AMOR1 Represents a Novel Thermostable Type of GH70 4,6-α-Glucanotransferase That Synthesizes a Linear Alternating (α1 → 6)/(α1 → 4) α-Glucan and Delays Bread Staling.
34427087	11	38	theme	Geobacillus	1440:1450	arg1	sp					1452:1453	this Geobacillus sp	1435:1453	this Geobacillus sp	1435:1453	Notably, this Geobacillus sp.
34427087	5	39	theme	Exiguobacterium	663:677	arg1	bacteria					640:647	Gram-positive bacteria	626:647	Gram-positive bacteria of the genera Exiguobacterium, Bacillus, and Geobacillus, adapted to growth at more extreme temperatures	626:752	Database searches showed that related GtfC enzymes occur in Gram-positive bacteria of the genera Exiguobacterium, Bacillus, and Geobacillus, adapted to growth at more extreme temperatures.
34427087	10	40	theme	Detailed	1229:1236	arg1	characterization					1249:1264	Detailed structural characterization	1229:1264	Detailed structural characterization of its starch-derived α-glucan products	1229:1304	Detailed structural characterization of its starch-derived α-glucan products revealed that it yielded a unique polymer with alternating (α1 → 6)/(α1 → 4)-linked glucose units but without branches.
34427087	10	41	theme	structural	1238:1247	arg1	characterization					1249:1264	Detailed structural characterization	1229:1264	Detailed structural characterization of its starch-derived α-glucan products	1229:1304	Detailed structural characterization of its starch-derived α-glucan products revealed that it yielded a unique polymer with alternating (α1 → 6)/(α1 → 4)-linked glucose units but without branches.
34427087	9	42	theme	novel	1201:1205	arg1	α-amylase					1218:1226	a novel maltogenic α-amylase	1199:1226	a novel maltogenic α-amylase	1199:1226	In fact, this GtfC represents a novel maltogenic α-amylase.
34427087	10	43	link	-linked	1382:1388	arg1	units					1398:1402	alternating (α1 → 6)/(α1 → 4)-linked glucose units	1353:1402	alternating (α1 → 6)/(α1 → 4)-linked glucose units	1353:1402	Detailed structural characterization of its starch-derived α-glucan products revealed that it yielded a unique polymer with alternating (α1 → 6)/(α1 → 4)-linked glucose units but without branches.
34427087	10	44	theme	glucose	1390:1396	arg1	units					1398:1402	alternating (α1 → 6)/(α1 → 4)-linked glucose units	1353:1402	alternating (α1 → 6)/(α1 → 4)-linked glucose units	1353:1402	Detailed structural characterization of its starch-derived α-glucan products revealed that it yielded a unique polymer with alternating (α1 → 6)/(α1 → 4)-linked glucose units but without branches.
34427087	8	45	theme	product	1029:1035	arg1	specificity					1037:1047	a novel product specificity	1021:1047	a novel product specificity	1021:1047	This thermostable 4,6-α-glucanotransferase has a novel product specificity, cleaving off predominantly maltose units from amylose, attaching them with an (α1 → 6)-linkage to acceptor substrates.
34427087	8	46	contain	has	1017:1019	arg2	specificity					1037:1047	a novel product specificity	1021:1047	a novel product specificity	1021:1047	This thermostable 4,6-α-glucanotransferase has a novel product specificity, cleaving off predominantly maltose units from amylose, attaching them with an (α1 → 6)-linkage to acceptor substrates.
34427087	8	46	contain	has	1017:1019	arg1	4,6-α-glucanotransferase					992:1015	This thermostable 4,6-α-glucanotransferase	974:1015	This thermostable 4,6-α-glucanotransferase	974:1015	This thermostable 4,6-α-glucanotransferase has a novel product specificity, cleaving off predominantly maltose units from amylose, attaching them with an (α1 → 6)-linkage to acceptor substrates.
34427087	0	47	theme	sp	27:28	arg1	Enzyme					5:10	Enzyme	5:10	Enzyme of Geobacillus sp	5:28	GtfC Enzyme of Geobacillus sp.
34427087	12	48	theme	clear	1483:1487	arg1	effects					1501:1507	clear antistaling effects	1483:1507	clear antistaling effects	1483:1507	12AMOR1 GtfC enzyme showed clear antistaling effects in bread bakery products.
34427087	5	49	theme	extreme	733:739	arg1	temperatures					741:752	more extreme temperatures	728:752	more extreme temperatures	728:752	Database searches showed that related GtfC enzymes occur in Gram-positive bacteria of the genera Exiguobacterium, Bacillus, and Geobacillus, adapted to growth at more extreme temperatures.
34427087	10	50	theme	products	1297:1304	arg1	characterization					1249:1264	Detailed structural characterization	1229:1264	Detailed structural characterization of its starch-derived α-glucan products	1229:1304	Detailed structural characterization of its starch-derived α-glucan products revealed that it yielded a unique polymer with alternating (α1 → 6)/(α1 → 4)-linked glucose units but without branches.
34427087	3	51	theme	family	418:423	arg1	4,3-α-glucanotransferases					361:385	4,3-α-glucanotransferases	361:385	4,3-α-glucanotransferases of the glycosyl hydrolase (GH) family 70 (subfamily GtfB)	361:443	In previous work, we have characterized various 4,6- and 4,3-α-glucanotransferases of the glycosyl hydrolase (GH) family 70 (subfamily GtfB), synthesizing linear or branched α-glucans.
34427087	3	51	theme	family	418:423	arg1	4,6-					352:355	various 4,6-	344:355	various 4,6-	344:355	In previous work, we have characterized various 4,6- and 4,3-α-glucanotransferases of the glycosyl hydrolase (GH) family 70 (subfamily GtfB), synthesizing linear or branched α-glucans.
34427087	5	52	theme	Gram-positive	626:638	arg1	bacteria					640:647	Gram-positive bacteria	626:647	Gram-positive bacteria of the genera Exiguobacterium, Bacillus, and Geobacillus, adapted to growth at more extreme temperatures	626:752	Database searches showed that related GtfC enzymes occur in Gram-positive bacteria of the genera Exiguobacterium, Bacillus, and Geobacillus, adapted to growth at more extreme temperatures.
34427087	6	53	theme	sp	806:807	arg1	characteristics					771:785	characteristics	771:785	characteristics of the Geobacillus sp	771:807	Here, we report characteristics of the Geobacillus sp.
34427087	7	54	theme	high	955:958	arg1	temperatures					960:971	relatively high temperatures	944:971	relatively high temperatures	944:971	12AMOR1 GtfC enzyme, with an optimal reaction temperature of 60 °C and a melting temperature of 68 °C, allowing starch conversions at relatively high temperatures.
34427087	8	55	theme	acceptor	1148:1155	arg1	substrates					1157:1166	acceptor substrates	1148:1166	acceptor substrates	1148:1166	This thermostable 4,6-α-glucanotransferase has a novel product specificity, cleaving off predominantly maltose units from amylose, attaching them with an (α1 → 6)-linkage to acceptor substrates.
34427087	7	56	with	enzyme	823:828	arg1	temperature					891:901	a melting temperature	881:901	a melting temperature of 68 °C	881:910	12AMOR1 GtfC enzyme, with an optimal reaction temperature of 60 °C and a melting temperature of 68 °C, allowing starch conversions at relatively high temperatures.
34427087	7	56	with	enzyme	823:828	arg1	temperature					856:866	an optimal reaction temperature	836:866	an optimal reaction temperature of 60 °C	836:875	12AMOR1 GtfC enzyme, with an optimal reaction temperature of 60 °C and a melting temperature of 68 °C, allowing starch conversions at relatively high temperatures.
34427087	6	57	theme	Geobacillus	794:804	arg1	sp					806:807	the Geobacillus sp	790:807	the Geobacillus sp	790:807	Here, we report characteristics of the Geobacillus sp.
34427087	1	58	theme	α1	148:149	arg1	Linear					128:133	a Linear Alternating	126:145	a Linear Alternating (α1 → 6)/	126:155	12AMOR1 Represents a Novel Thermostable Type of GH70 4,6-α-Glucanotransferase That Synthesizes a Linear Alternating (α1 → 6)/(α1 → 4) α-Glucan and Delays Bread Staling.
34427087	1	58	theme	α1	148:149	arg1	→					151:151	α1 → 6	148:153	α1 → 6	148:153	12AMOR1 Represents a Novel Thermostable Type of GH70 4,6-α-Glucanotransferase That Synthesizes a Linear Alternating (α1 → 6)/(α1 → 4) α-Glucan and Delays Bread Staling.
34427087	10	59	theme	starch-derived	1273:1286	arg1	products					1297:1304	its starch-derived α-glucan products	1269:1304	its starch-derived α-glucan products	1269:1304	Detailed structural characterization of its starch-derived α-glucan products revealed that it yielded a unique polymer with alternating (α1 → 6)/(α1 → 4)-linked glucose units but without branches.
34427087	10	60	theme	alternating	1353:1363	arg1	units					1398:1402	alternating (α1 → 6)/(α1 → 4)-linked glucose units	1353:1402	alternating (α1 → 6)/(α1 → 4)-linked glucose units	1353:1402	Detailed structural characterization of its starch-derived α-glucan products revealed that it yielded a unique polymer with alternating (α1 → 6)/(α1 → 4)-linked glucose units but without branches.
34427087	3	61	theme	subfamily	429:437	arg1	family					418:423	the glycosyl hydrolase (GH) family 70	390:426	the glycosyl hydrolase (GH) family 70 (subfamily GtfB)	390:443	In previous work, we have characterized various 4,6- and 4,3-α-glucanotransferases of the glycosyl hydrolase (GH) family 70 (subfamily GtfB), synthesizing linear or branched α-glucans.
34427087	3	61	theme	subfamily	429:437	arg1	GtfB					439:442	subfamily GtfB	429:442	subfamily GtfB	429:442	In previous work, we have characterized various 4,6- and 4,3-α-glucanotransferases of the glycosyl hydrolase (GH) family 70 (subfamily GtfB), synthesizing linear or branched α-glucans.
34427087	5	62	theme	Geobacillus	694:704	arg1	bacteria					640:647	Gram-positive bacteria	626:647	Gram-positive bacteria of the genera Exiguobacterium, Bacillus, and Geobacillus, adapted to growth at more extreme temperatures	626:752	Database searches showed that related GtfC enzymes occur in Gram-positive bacteria of the genera Exiguobacterium, Bacillus, and Geobacillus, adapted to growth at more extreme temperatures.
34427087	8	63	theme	maltose	1077:1083	arg1	units					1085:1089	maltose units	1077:1089	maltose units	1077:1089	This thermostable 4,6-α-glucanotransferase has a novel product specificity, cleaving off predominantly maltose units from amylose, attaching them with an (α1 → 6)-linkage to acceptor substrates.
34427087	1	64	dep	α-Glucan	165:172	arg1	Linear					128:133	a Linear Alternating	126:145	a Linear Alternating (α1 → 6)/	126:155	12AMOR1 Represents a Novel Thermostable Type of GH70 4,6-α-Glucanotransferase That Synthesizes a Linear Alternating (α1 → 6)/(α1 → 4) α-Glucan and Delays Bread Staling.
34427087	1	64	dep	α-Glucan	165:172	arg1	→					151:151	α1 → 6	148:153	α1 → 6	148:153	12AMOR1 Represents a Novel Thermostable Type of GH70 4,6-α-Glucanotransferase That Synthesizes a Linear Alternating (α1 → 6)/(α1 → 4) α-Glucan and Delays Bread Staling.
34427087	1	64	dep	α-Glucan	165:172	arg1	→					160:160	α1 → 4	157:162	α1 → 4	157:162	12AMOR1 Represents a Novel Thermostable Type of GH70 4,6-α-Glucanotransferase That Synthesizes a Linear Alternating (α1 → 6)/(α1 → 4) α-Glucan and Delays Bread Staling.
34427087	2	65	theme	food	289:292	arg1	industry					294:301	the food industry	285:301	the food industry	285:301	Starch-acting α-glucanotransferase enzymes are of great interest for applications in the food industry.
34427087	7	66	theme	optimal	839:845	arg1	temperature					856:866	an optimal reaction temperature	836:866	an optimal reaction temperature of 60 °C	836:875	12AMOR1 GtfC enzyme, with an optimal reaction temperature of 60 °C and a melting temperature of 68 °C, allowing starch conversions at relatively high temperatures.
34427087	4	67	theme	mesophilic	541:550	arg1	Lactobacilli					552:563	mesophilic Lactobacilli	541:563	mesophilic Lactobacilli	541:563	Thus far, GtfB enzymes have only been identified in mesophilic Lactobacilli.
34427087	10	68	link	starch-derived	1273:1286	arg1	products					1297:1304	its starch-derived α-glucan products	1269:1304	its starch-derived α-glucan products	1269:1304	Detailed structural characterization of its starch-derived α-glucan products revealed that it yielded a unique polymer with alternating (α1 → 6)/(α1 → 4)-linked glucose units but without branches.
34427087	2	69	theme	Starch-acting	200:212	arg1	enzymes					235:241	Starch-acting α-glucanotransferase enzymes	200:241	Starch-acting α-glucanotransferase enzymes	200:241	Starch-acting α-glucanotransferase enzymes are of great interest for applications in the food industry.
34427087	7	70	theme	starch	922:927	arg1	conversions					929:939	starch conversions	922:939	starch conversions	922:939	12AMOR1 GtfC enzyme, with an optimal reaction temperature of 60 °C and a melting temperature of 68 °C, allowing starch conversions at relatively high temperatures.
34427087	7	71	theme	reaction	847:854	arg1	temperature					856:866	an optimal reaction temperature	836:866	an optimal reaction temperature of 60 °C	836:875	12AMOR1 GtfC enzyme, with an optimal reaction temperature of 60 °C and a melting temperature of 68 °C, allowing starch conversions at relatively high temperatures.
34427087	1	72	theme	Bread	185:189	arg1	Staling					191:197	Bread Staling	185:197	Bread Staling	185:197	12AMOR1 Represents a Novel Thermostable Type of GH70 4,6-α-Glucanotransferase That Synthesizes a Linear Alternating (α1 → 6)/(α1 → 4) α-Glucan and Delays Bread Staling.
34427087	5	73	from	temperatures	741:752	arg1	growth					718:723	growth	718:723	growth at more extreme temperatures	718:752	Database searches showed that related GtfC enzymes occur in Gram-positive bacteria of the genera Exiguobacterium, Bacillus, and Geobacillus, adapted to growth at more extreme temperatures.
34427087	7	74	theme	°C	909:910	arg1	temperature					891:901	a melting temperature	881:901	a melting temperature of 68 °C	881:910	12AMOR1 GtfC enzyme, with an optimal reaction temperature of 60 °C and a melting temperature of 68 °C, allowing starch conversions at relatively high temperatures.
34427087	7	74	theme	°C	909:910	arg1	temperature					856:866	an optimal reaction temperature	836:866	an optimal reaction temperature of 60 °C	836:875	12AMOR1 GtfC enzyme, with an optimal reaction temperature of 60 °C and a melting temperature of 68 °C, allowing starch conversions at relatively high temperatures.
34427087	3	75	theme	branched	469:476	arg1	α-glucans					478:486	linear or branched α-glucans	459:486	linear or branched α-glucans	459:486	In previous work, we have characterized various 4,6- and 4,3-α-glucanotransferases of the glycosyl hydrolase (GH) family 70 (subfamily GtfB), synthesizing linear or branched α-glucans.
34427087	2	76	from	applications	269:280	arg1	industry					294:301	the food industry	285:301	the food industry	285:301	Starch-acting α-glucanotransferase enzymes are of great interest for applications in the food industry.
34427087	12	77	theme	GtfC	1464:1467	arg1	enzyme					1469:1474	12AMOR1 GtfC enzyme	1456:1474	12AMOR1 GtfC enzyme	1456:1474	12AMOR1 GtfC enzyme showed clear antistaling effects in bread bakery products.
34878294	12	0	theme	secondary	2043:2051	arg1	chromosomes					2053:2063	secondary chromosomes	2043:2063	secondary chromosomes	2043:2063	However, an increasing number of secondary chromosomes have been identified in various bacteria (e.g., plant symbiotic bacteria and human pathogens).
34878294	16	1	with	evolution	2951:2959	arg1	systems					3005:3011	multireplicon systems	2991:3011	multireplicon systems	2991:3011	These findings will promote the understanding of the evolution and function of bacteria with multireplicon systems.
34878294	4	2	theme	larger	569:574	arg1	chromosome					552:561	The second chromosome	541:561	The second chromosome	541:561	The second chromosome, much larger than plasmids and even megaplasmids, had rRNA and a disparity of 1% relative to the main chromosome in guanine-cytosine (GC) content.
34878294	15	3	theme	diverse	2847:2853	arg1	polysaccharides					2855:2869	more diverse polysaccharides	2842:2869	more diverse polysaccharides from marine environments	2842:2894	This study indicated that the second chromosomes of the genus Flammeovirga initially originated from an ancestral plasmid and subsequently expanded by gene duplication or by obtaining heterologous genes with functions, thus promoting host strains to adapt to complex living environments (e.g., to degrade more diverse polysaccharides from marine environments).
34878294	11	4	theme	typical	1883:1889	arg1	IMPORTANCE					1855:1864	IMPORTANCE	1855:1864	IMPORTANCE For decades	1855:1876	IMPORTANCE For decades, the typical bacterial genome has been thought to contain a single chromosome and a few small plasmids carrying nonessential genes.
34878294	11	4	theme	typical	1883:1889	arg1	genome					1901:1906	the typical bacterial genome	1879:1906	the typical bacterial genome	1879:1906	IMPORTANCE For decades, the typical bacterial genome has been thought to contain a single chromosome and a few small plasmids carrying nonessential genes.
34878294	9	5	theme	second	1642:1647	arg1	chromosomes					1649:1659	the second chromosomes	1638:1659	the second chromosomes	1638:1659	This study can be used as an example to explain possible formation mechanisms and functions of the second chromosomes, providing a reference for peer research on the second chromosomes.
34878294	16	6	theme	bacteria	2977:2984	arg1	function					2965:2972	function	2965:2972	function	2965:2972	These findings will promote the understanding of the evolution and function of bacteria with multireplicon systems.
34878294	16	6	theme	bacteria	2977:2984	arg1	evolution					2951:2959	evolution	2951:2959	evolution	2951:2959	These findings will promote the understanding of the evolution and function of bacteria with multireplicon systems.
34878294	12	7	theme	various	2089:2095	arg1	bacteria					2097:2104	various bacteria	2089:2104	various bacteria (e.g., plant symbiotic bacteria and human pathogens)	2089:2157	However, an increasing number of secondary chromosomes have been identified in various bacteria (e.g., plant symbiotic bacteria and human pathogens).
34878294	6	8	theme	primary	1158:1164	arg1	chromosomes					1166:1176	the primary chromosomes	1154:1176	the primary chromosomes	1154:1176	The second chromosomal evolution had a higher mutation rate than the primary chromosomes.
34878294	16	9	theme	multireplicon	2991:3003	arg1	systems					3005:3011	multireplicon systems	2991:3011	multireplicon systems	2991:3011	These findings will promote the understanding of the evolution and function of bacteria with multireplicon systems.
34878294	3	10	theme	second	521:526	arg1	chromosomes					528:538	multireplicons and second chromosomes	502:538	multireplicons and second chromosomes	502:538	These genomes all had multireplicons and second chromosomes.
34878294	11	11	contain	carrying	1981:1988	arg2	genes					2003:2007	nonessential genes	1990:2007	nonessential genes	1990:2007	IMPORTANCE For decades, the typical bacterial genome has been thought to contain a single chromosome and a few small plasmids carrying nonessential genes.
34878294	11	11	contain	carrying	1981:1988	arg1	plasmids					1972:1979	a few small plasmids	1960:1979	a few small plasmids carrying nonessential genes	1960:2007	IMPORTANCE For decades, the typical bacterial genome has been thought to contain a single chromosome and a few small plasmids carrying nonessential genes.
34878294	16	12	with	function	2965:2972	arg1	systems					3005:3011	multireplicon systems	2991:3011	multireplicon systems	2991:3011	These findings will promote the understanding of the evolution and function of bacteria with multireplicon systems.
34878294	4	13	theme	%	642:642	arg1	rRNA					617:620	rRNA	617:620	rRNA	617:620	The second chromosome, much larger than plasmids and even megaplasmids, had rRNA and a disparity of 1% relative to the main chromosome in guanine-cytosine (GC) content.
34878294	4	13	theme	%	642:642	arg1	disparity					628:636	a disparity	626:636	a disparity of 1% relative to the main chromosome in guanine-cytosine (GC) content	626:707	The second chromosome, much larger than plasmids and even megaplasmids, had rRNA and a disparity of 1% relative to the main chromosome in guanine-cytosine (GC) content.
34878294	15	14	dep	degrade	2834:2840	arg1	e.g.					2825:2828	e.g.	2825:2828	e.g.	2825:2828	This study indicated that the second chromosomes of the genus Flammeovirga initially originated from an ancestral plasmid and subsequently expanded by gene duplication or by obtaining heterologous genes with functions, thus promoting host strains to adapt to complex living environments (e.g., to degrade more diverse polysaccharides from marine environments).
34878294	8	15	from	roles	1435:1439	arg1	process					1467:1473	the bacterial survival process	1444:1473	the bacterial survival process	1444:1473	The primary chromosomes were more evolutionarily conserved, while the second chromosomes were more plastic, which might be related to their different roles in the bacterial survival process.
34878294	13	16	theme	multireplicon	2361:2373	arg1	system					2375:2380	a multireplicon system	2359:2380	a multireplicon system	2359:2380	This study reported three complete genomes of the polysaccharide-degrading marine bacterial genus Flammeovirga, revealed that they harbor two chromosomes, and further identified that the presence of a multireplicon system is a characteristic of complete Flammeovirga genomes.
34878294	6	17	theme	mutation	1135:1142	arg1	rate					1144:1147	a higher mutation rate	1126:1147	a higher mutation rate than the primary chromosomes	1126:1176	The second chromosomal evolution had a higher mutation rate than the primary chromosomes.
34878294	5	18	theme	excellent	1038:1046	arg1	capabilities					1048:1059	the strains' excellent capabilities	1025:1059	the strains' excellent capabilities	1025:1059	The largest chromosomes carried core genes for cellular processes, while the second chromosomes were enriched with genes involved in the transport and metabolism of inorganic ions and carbohydrates, particularly genes encoding glycoside hydrolases and polysaccharide lyases, which constituted the genetic basis for the strains' excellent capabilities to utilize polysaccharides.
34878294	10	19	theme	theoretical	1770:1780	arg1	support					1782:1788	theoretical support	1770:1788	theoretical support for using genomic data to mine tool-type carbohydrase resources	1770:1852	In particular, the second chromosomes were enriched in polysaccharide-degrading enzymes, which will provide theoretical support for using genomic data to mine tool-type carbohydrase resources.
34878294	12	20	theme	symbiotic	2119:2127	arg1	bacteria					2129:2136	plant symbiotic bacteria	2113:2136	plant symbiotic bacteria	2113:2136	However, an increasing number of secondary chromosomes have been identified in various bacteria (e.g., plant symbiotic bacteria and human pathogens).
34878294	11	21	theme	small	1966:1970	arg1	plasmids					1972:1979	a few small plasmids	1960:1979	a few small plasmids carrying nonessential genes	1960:2007	IMPORTANCE For decades, the typical bacterial genome has been thought to contain a single chromosome and a few small plasmids carrying nonessential genes.
34878294	8	22	theme	bacterial	1448:1456	arg1	process					1467:1473	the bacterial survival process	1444:1473	the bacterial survival process	1444:1473	The primary chromosomes were more evolutionarily conserved, while the second chromosomes were more plastic, which might be related to their different roles in the bacterial survival process.
34878294	14	23	from	knowledge	2468:2476	arg1	chromosomes					2491:2501	secondary chromosomes	2481:2501	secondary chromosomes	2481:2501	These sequences will add to our knowledge on secondary chromosomes, especially within Bacteroidetes.
34878294	8	24	theme	primary	1289:1295	arg1	chromosomes					1297:1307	The primary chromosomes	1285:1307	The primary chromosomes	1285:1307	The primary chromosomes were more evolutionarily conserved, while the second chromosomes were more plastic, which might be related to their different roles in the bacterial survival process.
34878294	10	25	theme	genomic	1800:1806	arg1	data					1808:1811	genomic data	1800:1811	genomic data to mine tool-type carbohydrase resources	1800:1852	In particular, the second chromosomes were enriched in polysaccharide-degrading enzymes, which will provide theoretical support for using genomic data to mine tool-type carbohydrase resources.
34878294	5	26	theme	inorganic	875:883	arg1	ions					885:888	inorganic ions	875:888	inorganic ions	875:888	The largest chromosomes carried core genes for cellular processes, while the second chromosomes were enriched with genes involved in the transport and metabolism of inorganic ions and carbohydrates, particularly genes encoding glycoside hydrolases and polysaccharide lyases, which constituted the genetic basis for the strains' excellent capabilities to utilize polysaccharides.
34878294	4	27	theme	relative	644:651	arg1	%					642:642	1%	641:642	1% relative to the main chromosome in guanine-cytosine (GC) content	641:707	The second chromosome, much larger than plasmids and even megaplasmids, had rRNA and a disparity of 1% relative to the main chromosome in guanine-cytosine (GC) content.
34878294	16	28	theme	evolution	2951:2959	arg1	understanding					2930:2942	the understanding	2926:2942	the understanding of the evolution and function of bacteria with multireplicon systems	2926:3011	These findings will promote the understanding of the evolution and function of bacteria with multireplicon systems.
34878294	1	29	theme	strains	128:134	arg1	%					113:113	Approximately 10%	97:113	Approximately 10% of bacterial strains	97:134	Approximately 10% of bacterial strains contain more than one chromosome; however, in contrast to the primary chromosomes, the mechanisms underlying the formation of the second chromosomes and the significance of their existence remain unclear.
34878294	1	29	theme	strains	128:134	arg1	strains					128:134	bacterial strains	118:134	bacterial strains	118:134	Approximately 10% of bacterial strains contain more than one chromosome; however, in contrast to the primary chromosomes, the mechanisms underlying the formation of the second chromosomes and the significance of their existence remain unclear.
34878294	5	30	theme	ions	885:888	arg1	metabolism					861:870	metabolism	861:870	metabolism	861:870	The largest chromosomes carried core genes for cellular processes, while the second chromosomes were enriched with genes involved in the transport and metabolism of inorganic ions and carbohydrates, particularly genes encoding glycoside hydrolases and polysaccharide lyases, which constituted the genetic basis for the strains' excellent capabilities to utilize polysaccharides.
34878294	5	30	theme	ions	885:888	arg1	transport					847:855	transport	847:855	transport	847:855	The largest chromosomes carried core genes for cellular processes, while the second chromosomes were enriched with genes involved in the transport and metabolism of inorganic ions and carbohydrates, particularly genes encoding glycoside hydrolases and polysaccharide lyases, which constituted the genetic basis for the strains' excellent capabilities to utilize polysaccharides.
34878294	16	31	theme	function	2965:2972	arg1	understanding					2930:2942	the understanding	2926:2942	the understanding of the evolution and function of bacteria with multireplicon systems	2926:3011	These findings will promote the understanding of the evolution and function of bacteria with multireplicon systems.
34878294	5	32	theme	largest	714:720	arg1	chromosomes					722:732	The largest chromosomes	710:732	The largest chromosomes	710:732	The largest chromosomes carried core genes for cellular processes, while the second chromosomes were enriched with genes involved in the transport and metabolism of inorganic ions and carbohydrates, particularly genes encoding glycoside hydrolases and polysaccharide lyases, which constituted the genetic basis for the strains' excellent capabilities to utilize polysaccharides.
34878294	10	33	theme	tool-type	1821:1829	arg1	resources					1844:1852	mine tool-type carbohydrase resources	1816:1852	mine tool-type carbohydrase resources	1816:1852	In particular, the second chromosomes were enriched in polysaccharide-degrading enzymes, which will provide theoretical support for using genomic data to mine tool-type carbohydrase resources.
34878294	2	34	theme	genus	473:477	arg1	maps					460:463	complete genome maps	444:463	complete genome maps of this genus	444:477	Species of the genus Flammeovirga are typical polysaccharide-degrading bacteria, and herein, we report complete genome maps of this genus.
34878294	13	35	theme	Flammeovirga	2258:2269	arg1	genomes					2195:2201	three complete genomes	2180:2201	three complete genomes of the polysaccharide-degrading marine bacterial genus Flammeovirga	2180:2269	This study reported three complete genomes of the polysaccharide-degrading marine bacterial genus Flammeovirga, revealed that they harbor two chromosomes, and further identified that the presence of a multireplicon system is a characteristic of complete Flammeovirga genomes.
34878294	4	36	theme	main	660:663	arg1	chromosome					665:674	the main chromosome	656:674	the main chromosome in guanine-cytosine (GC) content	656:707	The second chromosome, much larger than plasmids and even megaplasmids, had rRNA and a disparity of 1% relative to the main chromosome in guanine-cytosine (GC) content.
34878294	11	37	contain	contain	1928:1934	arg2	chromosome					1945:1954	a single chromosome	1936:1954	a single chromosome	1936:1954	IMPORTANCE For decades, the typical bacterial genome has been thought to contain a single chromosome and a few small plasmids carrying nonessential genes.
34878294	11	37	contain	contain	1928:1934	arg1	IMPORTANCE					1855:1864	IMPORTANCE	1855:1864	IMPORTANCE For decades	1855:1876	IMPORTANCE For decades, the typical bacterial genome has been thought to contain a single chromosome and a few small plasmids carrying nonessential genes.
34878294	11	37	contain	contain	1928:1934	arg2	plasmids					1972:1979	a few small plasmids	1960:1979	a few small plasmids carrying nonessential genes	1960:2007	IMPORTANCE For decades, the typical bacterial genome has been thought to contain a single chromosome and a few small plasmids carrying nonessential genes.
34878294	11	37	contain	contain	1928:1934	arg1	genome					1901:1906	the typical bacterial genome	1879:1906	the typical bacterial genome	1879:1906	IMPORTANCE For decades, the typical bacterial genome has been thought to contain a single chromosome and a few small plasmids carrying nonessential genes.
34878294	1	38	dep	chromosomes	206:216	arg1	contrast					182:189	contrast	182:189	contrast	182:189	Approximately 10% of bacterial strains contain more than one chromosome; however, in contrast to the primary chromosomes, the mechanisms underlying the formation of the second chromosomes and the significance of their existence remain unclear.
34878294	3	39	contain	had	498:500	arg1	genomes					486:492	These genomes	480:492	These genomes	480:492	These genomes all had multireplicons and second chromosomes.
34878294	3	39	contain	had	498:500	arg2	chromosomes					528:538	multireplicons and second chromosomes	502:538	multireplicons and second chromosomes	502:538	These genomes all had multireplicons and second chromosomes.
34878294	7	40	theme	second	1196:1201	arg1	chromosomes					1203:1213	the second chromosomes	1192:1213	the second chromosomes	1192:1213	Furthermore, the second chromosomes were also enriched in horizontal transfer genes and duplicated genes.
34878294	9	41	theme	formation	1533:1541	arg1	mechanisms					1543:1552	possible formation mechanisms	1524:1552	possible formation mechanisms	1524:1552	This study can be used as an example to explain possible formation mechanisms and functions of the second chromosomes, providing a reference for peer research on the second chromosomes.
34878294	15	42	theme	complex	2796:2802	arg1	environments					2811:2822	complex living environments	2796:2822	complex living environments (e.g., to degrade more diverse polysaccharides from marine environments)	2796:2895	This study indicated that the second chromosomes of the genus Flammeovirga initially originated from an ancestral plasmid and subsequently expanded by gene duplication or by obtaining heterologous genes with functions, thus promoting host strains to adapt to complex living environments (e.g., to degrade more diverse polysaccharides from marine environments).
34878294	2	43	theme	typical	379:385	arg1	bacteria					412:419	typical polysaccharide-degrading bacteria	379:419	typical polysaccharide-degrading bacteria	379:419	Species of the genus Flammeovirga are typical polysaccharide-degrading bacteria, and herein, we report complete genome maps of this genus.
34878294	2	43	theme	typical	379:385	arg1	Species					341:347	Species	341:347	Species of the genus Flammeovirga	341:373	Species of the genus Flammeovirga are typical polysaccharide-degrading bacteria, and herein, we report complete genome maps of this genus.
34878294	2	44	theme	Flammeovirga	362:373	arg1	Species					341:347	Species	341:347	Species of the genus Flammeovirga	341:373	Species of the genus Flammeovirga are typical polysaccharide-degrading bacteria, and herein, we report complete genome maps of this genus.
34878294	2	44	theme	Flammeovirga	362:373	arg1	bacteria					412:419	typical polysaccharide-degrading bacteria	379:419	typical polysaccharide-degrading bacteria	379:419	Species of the genus Flammeovirga are typical polysaccharide-degrading bacteria, and herein, we report complete genome maps of this genus.
34878294	15	45	theme	living	2804:2809	arg1	environments					2811:2822	complex living environments	2796:2822	complex living environments (e.g., to degrade more diverse polysaccharides from marine environments)	2796:2895	This study indicated that the second chromosomes of the genus Flammeovirga initially originated from an ancestral plasmid and subsequently expanded by gene duplication or by obtaining heterologous genes with functions, thus promoting host strains to adapt to complex living environments (e.g., to degrade more diverse polysaccharides from marine environments).
34878294	13	46	theme	bacterial	2242:2250	arg1	Flammeovirga					2258:2269	the polysaccharide-degrading marine bacterial genus Flammeovirga	2206:2269	the polysaccharide-degrading marine bacterial genus Flammeovirga	2206:2269	This study reported three complete genomes of the polysaccharide-degrading marine bacterial genus Flammeovirga, revealed that they harbor two chromosomes, and further identified that the presence of a multireplicon system is a characteristic of complete Flammeovirga genomes.
34878294	11	47	theme	single	1938:1943	arg1	chromosome					1945:1954	a single chromosome	1936:1954	a single chromosome	1936:1954	IMPORTANCE For decades, the typical bacterial genome has been thought to contain a single chromosome and a few small plasmids carrying nonessential genes.
34878294	1	48	theme	second	266:271	arg1	chromosomes					273:283	the second chromosomes	262:283	the second chromosomes	262:283	Approximately 10% of bacterial strains contain more than one chromosome; however, in contrast to the primary chromosomes, the mechanisms underlying the formation of the second chromosomes and the significance of their existence remain unclear.
34878294	9	49	theme	second	1575:1580	arg1	chromosomes					1582:1592	the second chromosomes	1571:1592	the second chromosomes	1571:1592	This study can be used as an example to explain possible formation mechanisms and functions of the second chromosomes, providing a reference for peer research on the second chromosomes.
34878294	13	50	theme	complete	2405:2412	arg1	genomes					2427:2433	complete Flammeovirga genomes	2405:2433	complete Flammeovirga genomes	2405:2433	This study reported three complete genomes of the polysaccharide-degrading marine bacterial genus Flammeovirga, revealed that they harbor two chromosomes, and further identified that the presence of a multireplicon system is a characteristic of complete Flammeovirga genomes.
34878294	2	51	theme	genome	453:458	arg1	maps					460:463	complete genome maps	444:463	complete genome maps of this genus	444:477	Species of the genus Flammeovirga are typical polysaccharide-degrading bacteria, and herein, we report complete genome maps of this genus.
34878294	0	52	theme	Genus	53:57	arg1	Flammeovirga					59:70	the Genus Flammeovirga	49:70	the Genus Flammeovirga	49:70	The Second Chromosome Promotes the Adaptation of the Genus Flammeovirga to Complex Environments.
34878294	5	53	theme	cellular	757:764	arg1	processes					766:774	cellular processes	757:774	cellular processes	757:774	The largest chromosomes carried core genes for cellular processes, while the second chromosomes were enriched with genes involved in the transport and metabolism of inorganic ions and carbohydrates, particularly genes encoding glycoside hydrolases and polysaccharide lyases, which constituted the genetic basis for the strains' excellent capabilities to utilize polysaccharides.
34878294	13	54	theme	polysaccharide-degrading	2210:2233	arg1	Flammeovirga					2258:2269	the polysaccharide-degrading marine bacterial genus Flammeovirga	2206:2269	the polysaccharide-degrading marine bacterial genus Flammeovirga	2206:2269	This study reported three complete genomes of the polysaccharide-degrading marine bacterial genus Flammeovirga, revealed that they harbor two chromosomes, and further identified that the presence of a multireplicon system is a characteristic of complete Flammeovirga genomes.
34878294	13	55	theme	genomes	2427:2433	arg1	characteristic					2387:2400	a characteristic	2385:2400	a characteristic of complete Flammeovirga genomes	2385:2433	This study reported three complete genomes of the polysaccharide-degrading marine bacterial genus Flammeovirga, revealed that they harbor two chromosomes, and further identified that the presence of a multireplicon system is a characteristic of complete Flammeovirga genomes.
34878294	13	55	theme	genomes	2427:2433	arg1	presence					2347:2354	the presence	2343:2354	the presence of a multireplicon system	2343:2380	This study reported three complete genomes of the polysaccharide-degrading marine bacterial genus Flammeovirga, revealed that they harbor two chromosomes, and further identified that the presence of a multireplicon system is a characteristic of complete Flammeovirga genomes.
34878294	10	56	theme	second	1681:1686	arg1	chromosomes					1688:1698	the second chromosomes	1677:1698	the second chromosomes	1677:1698	In particular, the second chromosomes were enriched in polysaccharide-degrading enzymes, which will provide theoretical support for using genomic data to mine tool-type carbohydrase resources.
34878294	15	57	theme	ancestral	2641:2649	arg1	plasmid					2651:2657	an ancestral plasmid	2638:2657	an ancestral plasmid	2638:2657	This study indicated that the second chromosomes of the genus Flammeovirga initially originated from an ancestral plasmid and subsequently expanded by gene duplication or by obtaining heterologous genes with functions, thus promoting host strains to adapt to complex living environments (e.g., to degrade more diverse polysaccharides from marine environments).
34878294	1	58	theme	primary	198:204	arg1	chromosomes					206:216	the primary chromosomes	194:216	the primary chromosomes	194:216	Approximately 10% of bacterial strains contain more than one chromosome; however, in contrast to the primary chromosomes, the mechanisms underlying the formation of the second chromosomes and the significance of their existence remain unclear.
34878294	7	59	theme	duplicated	1267:1276	arg1	genes					1278:1282	duplicated genes	1267:1282	duplicated genes	1267:1282	Furthermore, the second chromosomes were also enriched in horizontal transfer genes and duplicated genes.
34878294	11	60	theme	bacterial	1891:1899	arg1	IMPORTANCE					1855:1864	IMPORTANCE	1855:1864	IMPORTANCE For decades	1855:1876	IMPORTANCE For decades, the typical bacterial genome has been thought to contain a single chromosome and a few small plasmids carrying nonessential genes.
34878294	11	60	theme	bacterial	1891:1899	arg1	genome					1901:1906	the typical bacterial genome	1879:1906	the typical bacterial genome	1879:1906	IMPORTANCE For decades, the typical bacterial genome has been thought to contain a single chromosome and a few small plasmids carrying nonessential genes.
34878294	4	61	from	chromosome	665:674	arg1	content					701:707	guanine-cytosine (GC) content	679:707	guanine-cytosine (GC) content	679:707	The second chromosome, much larger than plasmids and even megaplasmids, had rRNA and a disparity of 1% relative to the main chromosome in guanine-cytosine (GC) content.
34878294	13	62	theme	complete	2186:2193	arg1	genomes					2195:2201	three complete genomes	2180:2201	three complete genomes of the polysaccharide-degrading marine bacterial genus Flammeovirga	2180:2269	This study reported three complete genomes of the polysaccharide-degrading marine bacterial genus Flammeovirga, revealed that they harbor two chromosomes, and further identified that the presence of a multireplicon system is a characteristic of complete Flammeovirga genomes.
34878294	12	63	theme	increasing	2022:2031	arg1	number					2033:2038	an increasing number	2019:2038	an increasing number of secondary chromosomes	2019:2063	However, an increasing number of secondary chromosomes have been identified in various bacteria (e.g., plant symbiotic bacteria and human pathogens).
34878294	12	64	dep	bacteria	2129:2136	arg1	e.g.					2107:2110	e.g.	2107:2110	e.g.	2107:2110	However, an increasing number of secondary chromosomes have been identified in various bacteria (e.g., plant symbiotic bacteria and human pathogens).
34878294	15	65	from	environments	2883:2894	arg1	polysaccharides					2855:2869	more diverse polysaccharides	2842:2869	more diverse polysaccharides from marine environments	2842:2894	This study indicated that the second chromosomes of the genus Flammeovirga initially originated from an ancestral plasmid and subsequently expanded by gene duplication or by obtaining heterologous genes with functions, thus promoting host strains to adapt to complex living environments (e.g., to degrade more diverse polysaccharides from marine environments).
34878294	7	66	theme	horizontal	1237:1246	arg1	genes					1257:1261	horizontal transfer genes	1237:1261	horizontal transfer genes	1237:1261	Furthermore, the second chromosomes were also enriched in horizontal transfer genes and duplicated genes.
34878294	13	67	theme	Flammeovirga	2414:2425	arg1	genomes					2427:2433	complete Flammeovirga genomes	2405:2433	complete Flammeovirga genomes	2405:2433	This study reported three complete genomes of the polysaccharide-degrading marine bacterial genus Flammeovirga, revealed that they harbor two chromosomes, and further identified that the presence of a multireplicon system is a characteristic of complete Flammeovirga genomes.
34878294	15	68	theme	genus	2593:2597	arg1	Flammeovirga					2599:2610	the genus Flammeovirga	2589:2610	the genus Flammeovirga	2589:2610	This study indicated that the second chromosomes of the genus Flammeovirga initially originated from an ancestral plasmid and subsequently expanded by gene duplication or by obtaining heterologous genes with functions, thus promoting host strains to adapt to complex living environments (e.g., to degrade more diverse polysaccharides from marine environments).
34878294	15	69	theme	host	2771:2774	arg1	strains					2776:2782	host strains	2771:2782	host strains	2771:2782	This study indicated that the second chromosomes of the genus Flammeovirga initially originated from an ancestral plasmid and subsequently expanded by gene duplication or by obtaining heterologous genes with functions, thus promoting host strains to adapt to complex living environments (e.g., to degrade more diverse polysaccharides from marine environments).
34878294	14	70	theme	secondary	2481:2489	arg1	chromosomes					2491:2501	secondary chromosomes	2481:2501	secondary chromosomes	2481:2501	These sequences will add to our knowledge on secondary chromosomes, especially within Bacteroidetes.
34878294	1	71	contain	contain	136:142	arg2	chromosome					158:167	more than one chromosome	144:167	more than one chromosome	144:167	Approximately 10% of bacterial strains contain more than one chromosome; however, in contrast to the primary chromosomes, the mechanisms underlying the formation of the second chromosomes and the significance of their existence remain unclear.
34878294	1	71	contain	contain	136:142	arg1	%					113:113	Approximately 10%	97:113	Approximately 10% of bacterial strains	97:134	Approximately 10% of bacterial strains contain more than one chromosome; however, in contrast to the primary chromosomes, the mechanisms underlying the formation of the second chromosomes and the significance of their existence remain unclear.
34878294	1	71	contain	contain	136:142	arg1	strains					128:134	bacterial strains	118:134	bacterial strains	118:134	Approximately 10% of bacterial strains contain more than one chromosome; however, in contrast to the primary chromosomes, the mechanisms underlying the formation of the second chromosomes and the significance of their existence remain unclear.
34878294	15	72	theme	gene	2688:2691	arg1	duplication					2693:2703	gene duplication	2688:2703	gene duplication	2688:2703	This study indicated that the second chromosomes of the genus Flammeovirga initially originated from an ancestral plasmid and subsequently expanded by gene duplication or by obtaining heterologous genes with functions, thus promoting host strains to adapt to complex living environments (e.g., to degrade more diverse polysaccharides from marine environments).
34878294	12	73	theme	human	2142:2146	arg1	pathogens					2148:2156	human pathogens	2142:2156	human pathogens	2142:2156	However, an increasing number of secondary chromosomes have been identified in various bacteria (e.g., plant symbiotic bacteria and human pathogens).
34878294	5	74	theme	second	787:792	arg1	chromosomes					794:804	the second chromosomes	783:804	the second chromosomes	783:804	The largest chromosomes carried core genes for cellular processes, while the second chromosomes were enriched with genes involved in the transport and metabolism of inorganic ions and carbohydrates, particularly genes encoding glycoside hydrolases and polysaccharide lyases, which constituted the genetic basis for the strains' excellent capabilities to utilize polysaccharides.
34878294	6	75	theme	higher	1128:1133	arg1	rate					1144:1147	a higher mutation rate	1126:1147	a higher mutation rate than the primary chromosomes	1126:1176	The second chromosomal evolution had a higher mutation rate than the primary chromosomes.
34878294	10	76	theme	mine	1816:1819	arg1	resources					1844:1852	mine tool-type carbohydrase resources	1816:1852	mine tool-type carbohydrase resources	1816:1852	In particular, the second chromosomes were enriched in polysaccharide-degrading enzymes, which will provide theoretical support for using genomic data to mine tool-type carbohydrase resources.
34878294	6	77	contain	had	1122:1124	arg1	evolution					1112:1120	The second chromosomal evolution	1089:1120	The second chromosomal evolution	1089:1120	The second chromosomal evolution had a higher mutation rate than the primary chromosomes.
34878294	6	77	contain	had	1122:1124	arg2	rate					1144:1147	a higher mutation rate	1126:1147	a higher mutation rate than the primary chromosomes	1126:1176	The second chromosomal evolution had a higher mutation rate than the primary chromosomes.
34878294	12	78	theme	plant	2113:2117	arg1	bacteria					2129:2136	plant symbiotic bacteria	2113:2136	plant symbiotic bacteria	2113:2136	However, an increasing number of secondary chromosomes have been identified in various bacteria (e.g., plant symbiotic bacteria and human pathogens).
34878294	15	79	theme	marine	2876:2881	arg1	environments					2883:2894	marine environments	2876:2894	marine environments	2876:2894	This study indicated that the second chromosomes of the genus Flammeovirga initially originated from an ancestral plasmid and subsequently expanded by gene duplication or by obtaining heterologous genes with functions, thus promoting host strains to adapt to complex living environments (e.g., to degrade more diverse polysaccharides from marine environments).
34878294	13	80	theme	system	2375:2380	arg1	characteristic					2387:2400	a characteristic	2385:2400	a characteristic of complete Flammeovirga genomes	2385:2433	This study reported three complete genomes of the polysaccharide-degrading marine bacterial genus Flammeovirga, revealed that they harbor two chromosomes, and further identified that the presence of a multireplicon system is a characteristic of complete Flammeovirga genomes.
34878294	13	80	theme	system	2375:2380	arg1	presence					2347:2354	the presence	2343:2354	the presence of a multireplicon system	2343:2380	This study reported three complete genomes of the polysaccharide-degrading marine bacterial genus Flammeovirga, revealed that they harbor two chromosomes, and further identified that the presence of a multireplicon system is a characteristic of complete Flammeovirga genomes.
34878294	11	81	theme	nonessential	1990:2001	arg1	genes					2003:2007	nonessential genes	1990:2007	nonessential genes	1990:2007	IMPORTANCE For decades, the typical bacterial genome has been thought to contain a single chromosome and a few small plasmids carrying nonessential genes.
34878294	1	82	theme	bacterial	118:126	arg1	strains					128:134	bacterial strains	118:134	bacterial strains	118:134	Approximately 10% of bacterial strains contain more than one chromosome; however, in contrast to the primary chromosomes, the mechanisms underlying the formation of the second chromosomes and the significance of their existence remain unclear.
34878294	4	83	theme	guanine-cytosine	679:694	arg1	content					701:707	guanine-cytosine (GC) content	679:707	guanine-cytosine (GC) content	679:707	The second chromosome, much larger than plasmids and even megaplasmids, had rRNA and a disparity of 1% relative to the main chromosome in guanine-cytosine (GC) content.
34878294	0	84	theme	Flammeovirga	59:70	arg1	Adaptation					35:44	the Adaptation	31:44	the Adaptation of the Genus Flammeovirga to Complex Environments	31:94	The Second Chromosome Promotes the Adaptation of the Genus Flammeovirga to Complex Environments.
34878294	10	85	theme	carbohydrase	1831:1842	arg1	resources					1844:1852	mine tool-type carbohydrase resources	1816:1852	mine tool-type carbohydrase resources	1816:1852	In particular, the second chromosomes were enriched in polysaccharide-degrading enzymes, which will provide theoretical support for using genomic data to mine tool-type carbohydrase resources.
34878294	5	86	contain	carried	734:740	arg1	chromosomes					722:732	The largest chromosomes	710:732	The largest chromosomes	710:732	The largest chromosomes carried core genes for cellular processes, while the second chromosomes were enriched with genes involved in the transport and metabolism of inorganic ions and carbohydrates, particularly genes encoding glycoside hydrolases and polysaccharide lyases, which constituted the genetic basis for the strains' excellent capabilities to utilize polysaccharides.
34878294	5	86	contain	carried	734:740	arg2	genes					747:751	core genes	742:751	core genes for cellular processes	742:774	The largest chromosomes carried core genes for cellular processes, while the second chromosomes were enriched with genes involved in the transport and metabolism of inorganic ions and carbohydrates, particularly genes encoding glycoside hydrolases and polysaccharide lyases, which constituted the genetic basis for the strains' excellent capabilities to utilize polysaccharides.
34878294	12	87	theme	chromosomes	2053:2063	arg1	number					2033:2038	an increasing number	2019:2038	an increasing number of secondary chromosomes	2019:2063	However, an increasing number of secondary chromosomes have been identified in various bacteria (e.g., plant symbiotic bacteria and human pathogens).
34878294	0	88	theme	Complex	75:81	arg1	Environments					83:94	Complex Environments	75:94	Complex Environments	75:94	The Second Chromosome Promotes the Adaptation of the Genus Flammeovirga to Complex Environments.
34878294	1	89	theme	existence	315:323	arg1	significance					293:304	the significance	289:304	the significance of their existence	289:323	Approximately 10% of bacterial strains contain more than one chromosome; however, in contrast to the primary chromosomes, the mechanisms underlying the formation of the second chromosomes and the significance of their existence remain unclear.
34878294	1	89	theme	existence	315:323	arg1	formation					249:257	the formation	245:257	the formation of the second chromosomes	245:283	Approximately 10% of bacterial strains contain more than one chromosome; however, in contrast to the primary chromosomes, the mechanisms underlying the formation of the second chromosomes and the significance of their existence remain unclear.
34878294	9	90	used	used	1494:1497	arg2	study					1481:1485	This study	1476:1485	This study	1476:1485	This study can be used as an example to explain possible formation mechanisms and functions of the second chromosomes, providing a reference for peer research on the second chromosomes.
34878294	9	90	used	used	1494:1497	arg2	example					1505:1511	an example	1502:1511	an example	1502:1511	This study can be used as an example to explain possible formation mechanisms and functions of the second chromosomes, providing a reference for peer research on the second chromosomes.
34878294	15	91	theme	heterologous	2721:2732	arg1	genes					2734:2738	heterologous genes	2721:2738	heterologous genes	2721:2738	This study indicated that the second chromosomes of the genus Flammeovirga initially originated from an ancestral plasmid and subsequently expanded by gene duplication or by obtaining heterologous genes with functions, thus promoting host strains to adapt to complex living environments (e.g., to degrade more diverse polysaccharides from marine environments).
34878294	9	92	theme	possible	1524:1531	arg1	mechanisms					1543:1552	possible formation mechanisms	1524:1552	possible formation mechanisms	1524:1552	This study can be used as an example to explain possible formation mechanisms and functions of the second chromosomes, providing a reference for peer research on the second chromosomes.
34878294	5	93	theme	glycoside	937:945	arg1	hydrolases					947:956	glycoside hydrolases	937:956	glycoside hydrolases	937:956	The largest chromosomes carried core genes for cellular processes, while the second chromosomes were enriched with genes involved in the transport and metabolism of inorganic ions and carbohydrates, particularly genes encoding glycoside hydrolases and polysaccharide lyases, which constituted the genetic basis for the strains' excellent capabilities to utilize polysaccharides.
34878294	6	94	theme	chromosomal	1100:1110	arg1	evolution					1112:1120	The second chromosomal evolution	1089:1120	The second chromosomal evolution	1089:1120	The second chromosomal evolution had a higher mutation rate than the primary chromosomes.
34878294	15	95	theme	second	2567:2572	arg1	chromosomes					2574:2584	the second chromosomes	2563:2584	the second chromosomes of the genus Flammeovirga	2563:2610	This study indicated that the second chromosomes of the genus Flammeovirga initially originated from an ancestral plasmid and subsequently expanded by gene duplication or by obtaining heterologous genes with functions, thus promoting host strains to adapt to complex living environments (e.g., to degrade more diverse polysaccharides from marine environments).
34878294	9	96	from	research	1626:1633	arg1	chromosomes					1649:1659	the second chromosomes	1638:1659	the second chromosomes	1638:1659	This study can be used as an example to explain possible formation mechanisms and functions of the second chromosomes, providing a reference for peer research on the second chromosomes.
34878294	8	97	theme	second	1355:1360	arg1	chromosomes					1362:1372	the second chromosomes	1351:1372	the second chromosomes	1351:1372	The primary chromosomes were more evolutionarily conserved, while the second chromosomes were more plastic, which might be related to their different roles in the bacterial survival process.
34878294	8	97	theme	second	1355:1360	arg1	plastic					1384:1390	plastic	1384:1390	plastic	1384:1390	The primary chromosomes were more evolutionarily conserved, while the second chromosomes were more plastic, which might be related to their different roles in the bacterial survival process.
34878294	0	98	theme	Second	4:9	arg1	Chromosome					11:20	The Second Chromosome	0:20	The Second Chromosome	0:20	The Second Chromosome Promotes the Adaptation of the Genus Flammeovirga to Complex Environments.
34878294	5	99	theme	carbohydrates	894:906	arg1	metabolism					861:870	metabolism	861:870	metabolism	861:870	The largest chromosomes carried core genes for cellular processes, while the second chromosomes were enriched with genes involved in the transport and metabolism of inorganic ions and carbohydrates, particularly genes encoding glycoside hydrolases and polysaccharide lyases, which constituted the genetic basis for the strains' excellent capabilities to utilize polysaccharides.
34878294	5	99	theme	carbohydrates	894:906	arg1	transport					847:855	transport	847:855	transport	847:855	The largest chromosomes carried core genes for cellular processes, while the second chromosomes were enriched with genes involved in the transport and metabolism of inorganic ions and carbohydrates, particularly genes encoding glycoside hydrolases and polysaccharide lyases, which constituted the genetic basis for the strains' excellent capabilities to utilize polysaccharides.
34878294	4	100	theme	second	545:550	arg1	chromosome					552:561	The second chromosome	541:561	The second chromosome	541:561	The second chromosome, much larger than plasmids and even megaplasmids, had rRNA and a disparity of 1% relative to the main chromosome in guanine-cytosine (GC) content.
34878294	4	101	theme	GC	697:698	arg1	content					701:707	guanine-cytosine (GC) content	679:707	guanine-cytosine (GC) content	679:707	The second chromosome, much larger than plasmids and even megaplasmids, had rRNA and a disparity of 1% relative to the main chromosome in guanine-cytosine (GC) content.
34878294	2	102	theme	genus	356:360	arg1	Flammeovirga					362:373	the genus Flammeovirga	352:373	the genus Flammeovirga	352:373	Species of the genus Flammeovirga are typical polysaccharide-degrading bacteria, and herein, we report complete genome maps of this genus.
34878294	13	103	theme	genus	2252:2256	arg1	Flammeovirga					2258:2269	the polysaccharide-degrading marine bacterial genus Flammeovirga	2206:2269	the polysaccharide-degrading marine bacterial genus Flammeovirga	2206:2269	This study reported three complete genomes of the polysaccharide-degrading marine bacterial genus Flammeovirga, revealed that they harbor two chromosomes, and further identified that the presence of a multireplicon system is a characteristic of complete Flammeovirga genomes.
34878294	11	104	theme	few	1962:1964	arg1	plasmids					1972:1979	a few small plasmids	1960:1979	a few small plasmids carrying nonessential genes	1960:2007	IMPORTANCE For decades, the typical bacterial genome has been thought to contain a single chromosome and a few small plasmids carrying nonessential genes.
34878294	16	105	dep	evolution	2951:2959	arg1	the					2947:2949	the	2947:2949	the	2947:2949	These findings will promote the understanding of the evolution and function of bacteria with multireplicon systems.
34878294	5	106	dep	transport	847:855	arg1	the					843:845	the	843:845	the	843:845	The largest chromosomes carried core genes for cellular processes, while the second chromosomes were enriched with genes involved in the transport and metabolism of inorganic ions and carbohydrates, particularly genes encoding glycoside hydrolases and polysaccharide lyases, which constituted the genetic basis for the strains' excellent capabilities to utilize polysaccharides.
34878294	6	107	theme	second	1093:1098	arg1	evolution					1112:1120	The second chromosomal evolution	1089:1120	The second chromosomal evolution	1089:1120	The second chromosomal evolution had a higher mutation rate than the primary chromosomes.
34878294	5	108	theme	core	742:745	arg1	genes					747:751	core genes	742:751	core genes for cellular processes	742:774	The largest chromosomes carried core genes for cellular processes, while the second chromosomes were enriched with genes involved in the transport and metabolism of inorganic ions and carbohydrates, particularly genes encoding glycoside hydrolases and polysaccharide lyases, which constituted the genetic basis for the strains' excellent capabilities to utilize polysaccharides.
34878294	1	109	theme	chromosomes	273:283	arg1	significance					293:304	the significance	289:304	the significance of their existence	289:323	Approximately 10% of bacterial strains contain more than one chromosome; however, in contrast to the primary chromosomes, the mechanisms underlying the formation of the second chromosomes and the significance of their existence remain unclear.
34878294	1	109	theme	chromosomes	273:283	arg1	formation					249:257	the formation	245:257	the formation of the second chromosomes	245:283	Approximately 10% of bacterial strains contain more than one chromosome; however, in contrast to the primary chromosomes, the mechanisms underlying the formation of the second chromosomes and the significance of their existence remain unclear.
34878294	9	110	theme	chromosomes	1582:1592	arg1	mechanisms					1543:1552	possible formation mechanisms	1524:1552	possible formation mechanisms	1524:1552	This study can be used as an example to explain possible formation mechanisms and functions of the second chromosomes, providing a reference for peer research on the second chromosomes.
34878294	9	110	theme	chromosomes	1582:1592	arg1	functions					1558:1566	functions	1558:1566	functions	1558:1566	This study can be used as an example to explain possible formation mechanisms and functions of the second chromosomes, providing a reference for peer research on the second chromosomes.
34878294	4	111	contain	had	613:615	arg1	chromosome					552:561	The second chromosome	541:561	The second chromosome	541:561	The second chromosome, much larger than plasmids and even megaplasmids, had rRNA and a disparity of 1% relative to the main chromosome in guanine-cytosine (GC) content.
34878294	4	111	contain	had	613:615	arg2	disparity					628:636	a disparity	626:636	a disparity of 1% relative to the main chromosome in guanine-cytosine (GC) content	626:707	The second chromosome, much larger than plasmids and even megaplasmids, had rRNA and a disparity of 1% relative to the main chromosome in guanine-cytosine (GC) content.
34878294	4	111	contain	had	613:615	arg2	rRNA					617:620	rRNA	617:620	rRNA	617:620	The second chromosome, much larger than plasmids and even megaplasmids, had rRNA and a disparity of 1% relative to the main chromosome in guanine-cytosine (GC) content.
34878294	2	112	theme	polysaccharide-degrading	387:410	arg1	bacteria					412:419	typical polysaccharide-degrading bacteria	379:419	typical polysaccharide-degrading bacteria	379:419	Species of the genus Flammeovirga are typical polysaccharide-degrading bacteria, and herein, we report complete genome maps of this genus.
34878294	2	112	theme	polysaccharide-degrading	387:410	arg1	Species					341:347	Species	341:347	Species of the genus Flammeovirga	341:373	Species of the genus Flammeovirga are typical polysaccharide-degrading bacteria, and herein, we report complete genome maps of this genus.
34878294	5	113	theme	genetic	1007:1013	arg1	basis					1015:1019	the genetic basis	1003:1019	the genetic basis for the strains' excellent capabilities to utilize polysaccharides	1003:1086	The largest chromosomes carried core genes for cellular processes, while the second chromosomes were enriched with genes involved in the transport and metabolism of inorganic ions and carbohydrates, particularly genes encoding glycoside hydrolases and polysaccharide lyases, which constituted the genetic basis for the strains' excellent capabilities to utilize polysaccharides.
34878294	2	114	theme	complete	444:451	arg1	maps					460:463	complete genome maps	444:463	complete genome maps of this genus	444:477	Species of the genus Flammeovirga are typical polysaccharide-degrading bacteria, and herein, we report complete genome maps of this genus.
34878294	8	115	theme	survival	1458:1465	arg1	process					1467:1473	the bacterial survival process	1444:1473	the bacterial survival process	1444:1473	The primary chromosomes were more evolutionarily conserved, while the second chromosomes were more plastic, which might be related to their different roles in the bacterial survival process.
34878294	13	116	theme	marine	2235:2240	arg1	Flammeovirga					2258:2269	the polysaccharide-degrading marine bacterial genus Flammeovirga	2206:2269	the polysaccharide-degrading marine bacterial genus Flammeovirga	2206:2269	This study reported three complete genomes of the polysaccharide-degrading marine bacterial genus Flammeovirga, revealed that they harbor two chromosomes, and further identified that the presence of a multireplicon system is a characteristic of complete Flammeovirga genomes.
34878294	5	117	theme	polysaccharide	962:975	arg1	lyases					977:982	polysaccharide lyases	962:982	polysaccharide lyases	962:982	The largest chromosomes carried core genes for cellular processes, while the second chromosomes were enriched with genes involved in the transport and metabolism of inorganic ions and carbohydrates, particularly genes encoding glycoside hydrolases and polysaccharide lyases, which constituted the genetic basis for the strains' excellent capabilities to utilize polysaccharides.
34878294	4	118	theme	even	594:597	arg1	megaplasmids					599:610	even megaplasmids	594:610	even megaplasmids	594:610	The second chromosome, much larger than plasmids and even megaplasmids, had rRNA and a disparity of 1% relative to the main chromosome in guanine-cytosine (GC) content.
34878294	3	119	theme	multireplicons	502:515	arg1	chromosomes					528:538	multireplicons and second chromosomes	502:538	multireplicons and second chromosomes	502:538	These genomes all had multireplicons and second chromosomes.
34878294	9	120	theme	peer	1621:1624	arg1	research					1626:1633	peer research	1621:1633	peer research on the second chromosomes	1621:1659	This study can be used as an example to explain possible formation mechanisms and functions of the second chromosomes, providing a reference for peer research on the second chromosomes.
34878294	7	121	theme	transfer	1248:1255	arg1	genes					1257:1261	horizontal transfer genes	1237:1261	horizontal transfer genes	1237:1261	Furthermore, the second chromosomes were also enriched in horizontal transfer genes and duplicated genes.
34878294	8	122	theme	different	1425:1433	arg1	roles					1435:1439	their different roles	1419:1439	their different roles in the bacterial survival process	1419:1473	The primary chromosomes were more evolutionarily conserved, while the second chromosomes were more plastic, which might be related to their different roles in the bacterial survival process.
34878294	10	123	theme	polysaccharide-degrading	1717:1740	arg1	enzymes					1742:1748	polysaccharide-degrading enzymes	1717:1748	polysaccharide-degrading enzymes	1717:1748	In particular, the second chromosomes were enriched in polysaccharide-degrading enzymes, which will provide theoretical support for using genomic data to mine tool-type carbohydrase resources.
34878294	15	124	theme	Flammeovirga	2599:2610	arg1	chromosomes					2574:2584	the second chromosomes	2563:2584	the second chromosomes of the genus Flammeovirga	2563:2610	This study indicated that the second chromosomes of the genus Flammeovirga initially originated from an ancestral plasmid and subsequently expanded by gene duplication or by obtaining heterologous genes with functions, thus promoting host strains to adapt to complex living environments (e.g., to degrade more diverse polysaccharides from marine environments).
34130289	4	0	theme	human	528:532	arg1	milk					534:537	human milk	528:537	human milk	528:537	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	9	1	theme	milk	1154:1157	arg1	control					1159:1165	bovine milk control	1147:1165	bovine milk control (15.3 ± 8.2)	1147:1178	In terms of %SHL, negative control (7.7 ± 3.1), human milk control (13.3 ± 7.5), bovine milk control (15.3 ± 8.2), human milk (7.5 ± 5.0), and bovine milk (8.7 ± 6.3) did not differ among them (p > 0.05) but differed (p < 0.05) from sucrose (55.1 ± 5.4).
34130289	9	1	theme	milk	1154:1157	arg1	±					1173:1173	15.3 ± 8.2	1168:1177	15.3 ± 8.2	1168:1177	In terms of %SHL, negative control (7.7 ± 3.1), human milk control (13.3 ± 7.5), bovine milk control (15.3 ± 8.2), human milk (7.5 ± 5.0), and bovine milk (8.7 ± 6.3) did not differ among them (p > 0.05) but differed (p < 0.05) from sucrose (55.1 ± 5.4).
34130289	9	2	theme	p	1284:1284	arg1	<					1286:1286	p < 0.05	1284:1291	p < 0.05	1284:1291	In terms of %SHL, negative control (7.7 ± 3.1), human milk control (13.3 ± 7.5), bovine milk control (15.3 ± 8.2), human milk (7.5 ± 5.0), and bovine milk (8.7 ± 6.3) did not differ among them (p > 0.05) but differed (p < 0.05) from sucrose (55.1 ± 5.4).
34130289	4	3	theme	milk	580:583	arg1	control					585:591	active human milk control	567:591	active human milk control	567:591	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	4	3	theme	milk	580:583	arg1	lactose					558:564	7.0% lactose	553:564	7.0% lactose (active human milk control)	553:592	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	5	4	theme	culture	677:683	arg1	medium					685:690	The culture medium	673:690	The culture medium	673:690	The culture medium was changed twice daily, and the pH was analyzed as an indicator of biofilm acidogenicity.
34130289	1	5	theme	debatable	155:163	arg1	subject					165:171	a debatable subject	153:171	a debatable subject	153:171	The higher cariogenicity of human milk when compared with bovine milk is still a debatable subject.
34130289	1	5	theme	debatable	155:163	arg1	cariogenicity					85:97	The higher cariogenicity	74:97	The higher cariogenicity of human milk when compared with bovine milk	74:142	The higher cariogenicity of human milk when compared with bovine milk is still a debatable subject.
34130289	10	6	theme	%	1362:1362	arg1	demineralization					1344:1359	enamel demineralization	1337:1359	enamel demineralization (%SHL)	1337:1366	The findings of enamel demineralization (%SHL) were statistically supported by the data of biofilm acidogenicity, bacterial counts and EPS biofilm composition.
34130289	10	6	theme	%	1362:1362	arg1	SHL					1363:1365	%SHL	1362:1365	%SHL	1362:1365	The findings of enamel demineralization (%SHL) were statistically supported by the data of biofilm acidogenicity, bacterial counts and EPS biofilm composition.
34130289	10	7	theme	demineralization	1344:1359	arg1	findings					1325:1332	The findings	1321:1332	The findings of enamel demineralization (%SHL)	1321:1366	The findings of enamel demineralization (%SHL) were statistically supported by the data of biofilm acidogenicity, bacterial counts and EPS biofilm composition.
34130289	6	8	theme	growth	798:803	arg1	h					793:793	120 h	789:793	120 h of growth	789:803	After 120 h of growth, biofilms were harvested to evaluate viable cells, and soluble and insoluble extracellular polysaccharides (EPS).
34130289	4	9	theme	active	567:572	arg1	control					585:591	active human milk control	567:591	active human milk control	567:591	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	4	9	theme	active	567:572	arg1	lactose					558:564	7.0% lactose	553:564	7.0% lactose (active human milk control)	553:592	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	7	10	theme	%	1000:1000	arg1	loss					994:997	surface hardness loss	977:997	surface hardness loss (%SHL)	977:1004	Enamel demineralization was assessed by the percentage of surface hardness loss (%SHL).
34130289	7	10	theme	%	1000:1000	arg1	SHL					1001:1003	%SHL	1000:1003	%SHL	1000:1003	Enamel demineralization was assessed by the percentage of surface hardness loss (%SHL).
34130289	9	11	theme	human	1181:1185	arg1	milk					1187:1190	human milk	1181:1190	human milk (7.5 ± 5.0)	1181:1202	In terms of %SHL, negative control (7.7 ± 3.1), human milk control (13.3 ± 7.5), bovine milk control (15.3 ± 8.2), human milk (7.5 ± 5.0), and bovine milk (8.7 ± 6.3) did not differ among them (p > 0.05) but differed (p < 0.05) from sucrose (55.1 ± 5.4).
34130289	9	11	theme	human	1181:1185	arg1	±					1197:1197	7.5 ± 5.0	1193:1201	7.5 ± 5.0	1193:1201	In terms of %SHL, negative control (7.7 ± 3.1), human milk control (13.3 ± 7.5), bovine milk control (15.3 ± 8.2), human milk (7.5 ± 5.0), and bovine milk (8.7 ± 6.3) did not differ among them (p > 0.05) but differed (p < 0.05) from sucrose (55.1 ± 5.4).
34130289	11	12	contain	have	1541:1544	arg1	milk					1536:1539	human and bovine milk	1519:1539	human and bovine milk	1519:1539	This experimental study suggests that human and bovine milk have low cariogenic potential to provoke caries lesions in enamel.
34130289	11	12	contain	have	1541:1544	arg2	potential					1561:1569	low cariogenic potential	1546:1569	low cariogenic potential to provoke caries lesions in enamel	1546:1605	This experimental study suggests that human and bovine milk have low cariogenic potential to provoke caries lesions in enamel.
34130289	4	13	theme	active	609:614	arg1	control					628:634	active bovine milk control	609:634	active bovine milk control	609:634	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	4	13	theme	active	609:614	arg1	lactose					600:606	4.5% lactose	595:606	4.5% lactose (active bovine milk control)	595:635	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	11	14	theme	bovine	1529:1534	arg1	milk					1536:1539	human and bovine milk	1519:1539	human and bovine milk	1519:1539	This experimental study suggests that human and bovine milk have low cariogenic potential to provoke caries lesions in enamel.
34130289	5	15	theme	biofilm	760:766	arg1	acidogenicity					768:780	biofilm acidogenicity	760:780	biofilm acidogenicity	760:780	The culture medium was changed twice daily, and the pH was analyzed as an indicator of biofilm acidogenicity.
34130289	4	16	theme	milk	623:626	arg1	control					628:634	active bovine milk control	609:634	active bovine milk control	609:634	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	4	16	theme	milk	623:626	arg1	lactose					600:606	4.5% lactose	595:606	4.5% lactose (active bovine milk control)	595:635	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	2	17	theme	milk	228:231	arg1	exposure					233:240	human or bovine milk exposure	212:240	human or bovine milk exposure	212:240	Therefore, we evaluated the effect of human or bovine milk exposure on biofilm composition and enamel demineralization using a validated cariogenic biofilm model.
34130289	4	18	theme	4.5	595:597	arg1	%					598:598	%	598:598	%	598:598	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	0	19	theme	Enamel	49:54	arg1	Demineralization					56:71	Enamel Demineralization	49:71	Enamel Demineralization	49:71	Cariogenic Potential of Human and Bovine Milk on Enamel Demineralization.
34130289	11	20	theme	cariogenic	1550:1559	arg1	potential					1561:1569	low cariogenic potential	1546:1569	low cariogenic potential to provoke caries lesions in enamel	1546:1605	This experimental study suggests that human and bovine milk have low cariogenic potential to provoke caries lesions in enamel.
34130289	9	21	theme	%	1078:1078	arg1	SHL					1079:1081	%SHL	1078:1081	%SHL	1078:1081	In terms of %SHL, negative control (7.7 ± 3.1), human milk control (13.3 ± 7.5), bovine milk control (15.3 ± 8.2), human milk (7.5 ± 5.0), and bovine milk (8.7 ± 6.3) did not differ among them (p > 0.05) but differed (p < 0.05) from sucrose (55.1 ± 5.4).
34130289	3	22	theme	mutans	351:356	arg1	n					374:374	n = 8	374:378	n = 8	374:378	Streptococcus mutans UA159 biofilms (n = 8) were grown on human saliva-coated bovine enamel slabs of known surface hardness.
34130289	3	22	theme	mutans	351:356	arg1	biofilms					364:371	Streptococcus mutans UA159 biofilms	337:371	Streptococcus mutans UA159 biofilms (n = 8)	337:379	Streptococcus mutans UA159 biofilms (n = 8) were grown on human saliva-coated bovine enamel slabs of known surface hardness.
34130289	4	23	theme	%	643:643	arg1	sucrose					645:651	10% sucrose	641:651	10% sucrose (positive control)	641:670	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	4	23	theme	%	643:643	arg1	control					663:669	positive control	654:669	positive control	654:669	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	2	24	from	effect	202:207	arg1	composition					253:263	biofilm composition	245:263	biofilm composition	245:263	Therefore, we evaluated the effect of human or bovine milk exposure on biofilm composition and enamel demineralization using a validated cariogenic biofilm model.
34130289	2	24	from	effect	202:207	arg1	demineralization					276:291	enamel demineralization	269:291	enamel demineralization	269:291	Therefore, we evaluated the effect of human or bovine milk exposure on biofilm composition and enamel demineralization using a validated cariogenic biofilm model.
34130289	11	25	theme	human	1519:1523	arg1	milk					1536:1539	human and bovine milk	1519:1539	human and bovine milk	1519:1539	This experimental study suggests that human and bovine milk have low cariogenic potential to provoke caries lesions in enamel.
34130289	3	26	theme	saliva-coated	401:413	arg1	slabs					429:433	human saliva-coated bovine enamel slabs	395:433	human saliva-coated bovine enamel slabs of known surface hardness	395:459	Streptococcus mutans UA159 biofilms (n = 8) were grown on human saliva-coated bovine enamel slabs of known surface hardness.
34130289	0	27	theme	Bovine	34:39	arg1	Milk					41:44	Human and Bovine Milk	24:44	Human and Bovine Milk	24:44	Cariogenic Potential of Human and Bovine Milk on Enamel Demineralization.
34130289	0	28	theme	Cariogenic	0:9	arg1	Potential					11:19	Cariogenic Potential	0:19	Cariogenic Potential of Human and Bovine Milk on Enamel Demineralization.	0:72	Cariogenic Potential of Human and Bovine Milk on Enamel Demineralization.
34130289	11	29	theme	low	1546:1548	arg1	potential					1561:1569	low cariogenic potential	1546:1569	low cariogenic potential to provoke caries lesions in enamel	1546:1605	This experimental study suggests that human and bovine milk have low cariogenic potential to provoke caries lesions in enamel.
34130289	1	30	theme	human	102:106	arg1	milk					108:111	human milk	102:111	human milk	102:111	The higher cariogenicity of human milk when compared with bovine milk is still a debatable subject.
34130289	6	31	theme	extracellular	882:894	arg1	polysaccharides					896:910	extracellular polysaccharides	882:910	extracellular polysaccharides	882:910	After 120 h of growth, biofilms were harvested to evaluate viable cells, and soluble and insoluble extracellular polysaccharides (EPS).
34130289	3	32	theme	enamel	422:427	arg1	slabs					429:433	human saliva-coated bovine enamel slabs	395:433	human saliva-coated bovine enamel slabs of known surface hardness	395:459	Streptococcus mutans UA159 biofilms (n = 8) were grown on human saliva-coated bovine enamel slabs of known surface hardness.
34130289	8	33	theme	α	1057:1057	arg1	%					1062:1062	α = 5%	1057:1062	α = 5%	1057:1062	Data were analyzed by one-way ANOVA/Tukey's test (α = 5%).
34130289	8	33	theme	α	1057:1057	arg1	test					1051:1054	one-way ANOVA/Tukey's test	1029:1054	one-way ANOVA/Tukey's test (α = 5%)	1029:1063	Data were analyzed by one-way ANOVA/Tukey's test (α = 5%).
34130289	10	34	theme	biofilm	1412:1418	arg1	acidogenicity					1420:1432	biofilm acidogenicity	1412:1432	biofilm acidogenicity	1412:1432	The findings of enamel demineralization (%SHL) were statistically supported by the data of biofilm acidogenicity, bacterial counts and EPS biofilm composition.
34130289	7	35	theme	hardness	985:992	arg1	loss					994:997	surface hardness loss	977:997	surface hardness loss (%SHL)	977:1004	Enamel demineralization was assessed by the percentage of surface hardness loss (%SHL).
34130289	7	35	theme	hardness	985:992	arg1	SHL					1001:1003	%SHL	1000:1003	%SHL	1000:1003	Enamel demineralization was assessed by the percentage of surface hardness loss (%SHL).
34130289	9	36	theme	milk	1120:1123	arg1	±					1139:1139	13.3 ± 7.5	1134:1143	13.3 ± 7.5	1134:1143	In terms of %SHL, negative control (7.7 ± 3.1), human milk control (13.3 ± 7.5), bovine milk control (15.3 ± 8.2), human milk (7.5 ± 5.0), and bovine milk (8.7 ± 6.3) did not differ among them (p > 0.05) but differed (p < 0.05) from sucrose (55.1 ± 5.4).
34130289	9	36	theme	milk	1120:1123	arg1	control					1125:1131	human milk control	1114:1131	human milk control (13.3 ± 7.5)	1114:1144	In terms of %SHL, negative control (7.7 ± 3.1), human milk control (13.3 ± 7.5), bovine milk control (15.3 ± 8.2), human milk (7.5 ± 5.0), and bovine milk (8.7 ± 6.3) did not differ among them (p > 0.05) but differed (p < 0.05) from sucrose (55.1 ± 5.4).
34130289	4	37	theme	%	501:501	arg1	NaCl					503:506	0.9% NaCl	498:506	0.9% NaCl (negative control)	498:525	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	4	37	theme	%	501:501	arg1	control					518:524	negative control	509:524	negative control	509:524	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	10	38	theme	EPS	1456:1458	arg1	composition					1468:1478	EPS biofilm composition	1456:1478	EPS biofilm composition	1456:1478	The findings of enamel demineralization (%SHL) were statistically supported by the data of biofilm acidogenicity, bacterial counts and EPS biofilm composition.
34130289	2	39	theme	cariogenic	311:320	arg1	model					330:334	a validated cariogenic biofilm model	299:334	a validated cariogenic biofilm model	299:334	Therefore, we evaluated the effect of human or bovine milk exposure on biofilm composition and enamel demineralization using a validated cariogenic biofilm model.
34130289	11	40	theme	caries	1582:1587	arg1	lesions					1589:1595	caries lesions	1582:1595	caries lesions	1582:1595	This experimental study suggests that human and bovine milk have low cariogenic potential to provoke caries lesions in enamel.
34130289	9	41	theme	p	1260:1260	arg1	>					1262:1262	p > 0.05	1260:1267	p > 0.05	1260:1267	In terms of %SHL, negative control (7.7 ± 3.1), human milk control (13.3 ± 7.5), bovine milk control (15.3 ± 8.2), human milk (7.5 ± 5.0), and bovine milk (8.7 ± 6.3) did not differ among them (p > 0.05) but differed (p < 0.05) from sucrose (55.1 ± 5.4).
34130289	4	42	theme	bovine	540:545	arg1	milk					547:550	bovine milk	540:550	bovine milk	540:550	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	10	43	theme	counts	1445:1450	arg1	data					1404:1407	the data	1400:1407	the data of biofilm acidogenicity, bacterial counts and EPS biofilm composition	1400:1478	The findings of enamel demineralization (%SHL) were statistically supported by the data of biofilm acidogenicity, bacterial counts and EPS biofilm composition.
34130289	10	44	theme	composition	1468:1478	arg1	data					1404:1407	the data	1400:1407	the data of biofilm acidogenicity, bacterial counts and EPS biofilm composition	1400:1478	The findings of enamel demineralization (%SHL) were statistically supported by the data of biofilm acidogenicity, bacterial counts and EPS biofilm composition.
34130289	10	45	theme	enamel	1337:1342	arg1	demineralization					1344:1359	enamel demineralization	1337:1359	enamel demineralization (%SHL)	1337:1366	The findings of enamel demineralization (%SHL) were statistically supported by the data of biofilm acidogenicity, bacterial counts and EPS biofilm composition.
34130289	10	45	theme	enamel	1337:1342	arg1	SHL					1363:1365	%SHL	1362:1365	%SHL	1362:1365	The findings of enamel demineralization (%SHL) were statistically supported by the data of biofilm acidogenicity, bacterial counts and EPS biofilm composition.
34130289	9	46	theme	bovine	1147:1152	arg1	control					1159:1165	bovine milk control	1147:1165	bovine milk control (15.3 ± 8.2)	1147:1178	In terms of %SHL, negative control (7.7 ± 3.1), human milk control (13.3 ± 7.5), bovine milk control (15.3 ± 8.2), human milk (7.5 ± 5.0), and bovine milk (8.7 ± 6.3) did not differ among them (p > 0.05) but differed (p < 0.05) from sucrose (55.1 ± 5.4).
34130289	9	46	theme	bovine	1147:1152	arg1	±					1173:1173	15.3 ± 8.2	1168:1177	15.3 ± 8.2	1168:1177	In terms of %SHL, negative control (7.7 ± 3.1), human milk control (13.3 ± 7.5), bovine milk control (15.3 ± 8.2), human milk (7.5 ± 5.0), and bovine milk (8.7 ± 6.3) did not differ among them (p > 0.05) but differed (p < 0.05) from sucrose (55.1 ± 5.4).
34130289	2	47	theme	biofilm	245:251	arg1	composition					253:263	biofilm composition	245:263	biofilm composition	245:263	Therefore, we evaluated the effect of human or bovine milk exposure on biofilm composition and enamel demineralization using a validated cariogenic biofilm model.
34130289	0	48	theme	Milk	41:44	arg1	Potential					11:19	Cariogenic Potential	0:19	Cariogenic Potential of Human and Bovine Milk on Enamel Demineralization.	0:72	Cariogenic Potential of Human and Bovine Milk on Enamel Demineralization.
34130289	4	49	theme	human	574:578	arg1	control					585:591	active human milk control	567:591	active human milk control	567:591	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	4	49	theme	human	574:578	arg1	lactose					558:564	7.0% lactose	553:564	7.0% lactose (active human milk control)	553:592	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	7	50	theme	Enamel	919:924	arg1	demineralization					926:941	Enamel demineralization	919:941	Enamel demineralization	919:941	Enamel demineralization was assessed by the percentage of surface hardness loss (%SHL).
34130289	9	51	dep	differ	1241:1246	arg1	>					1262:1262	p > 0.05	1260:1267	p > 0.05	1260:1267	In terms of %SHL, negative control (7.7 ± 3.1), human milk control (13.3 ± 7.5), bovine milk control (15.3 ± 8.2), human milk (7.5 ± 5.0), and bovine milk (8.7 ± 6.3) did not differ among them (p > 0.05) but differed (p < 0.05) from sucrose (55.1 ± 5.4).
34130289	4	52	theme	%	556:556	arg1	control					585:591	active human milk control	567:591	active human milk control	567:591	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	4	52	theme	%	556:556	arg1	lactose					558:564	7.0% lactose	553:564	7.0% lactose (active human milk control)	553:592	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	2	53	theme	human	212:216	arg1	exposure					233:240	human or bovine milk exposure	212:240	human or bovine milk exposure	212:240	Therefore, we evaluated the effect of human or bovine milk exposure on biofilm composition and enamel demineralization using a validated cariogenic biofilm model.
34130289	8	54	theme	=	1059:1059	arg1	%					1062:1062	α = 5%	1057:1062	α = 5%	1057:1062	Data were analyzed by one-way ANOVA/Tukey's test (α = 5%).
34130289	8	54	theme	=	1059:1059	arg1	test					1051:1054	one-way ANOVA/Tukey's test	1029:1054	one-way ANOVA/Tukey's test (α = 5%)	1029:1063	Data were analyzed by one-way ANOVA/Tukey's test (α = 5%).
34130289	8	55	theme	one-way	1029:1035	arg1	%					1062:1062	α = 5%	1057:1062	α = 5%	1057:1062	Data were analyzed by one-way ANOVA/Tukey's test (α = 5%).
34130289	8	55	theme	one-way	1029:1035	arg1	test					1051:1054	one-way ANOVA/Tukey's test	1029:1054	one-way ANOVA/Tukey's test (α = 5%)	1029:1063	Data were analyzed by one-way ANOVA/Tukey's test (α = 5%).
34130289	1	56	theme	milk	108:111	arg1	subject					165:171	a debatable subject	153:171	a debatable subject	153:171	The higher cariogenicity of human milk when compared with bovine milk is still a debatable subject.
34130289	1	56	theme	milk	108:111	arg1	cariogenicity					85:97	The higher cariogenicity	74:97	The higher cariogenicity of human milk when compared with bovine milk	74:142	The higher cariogenicity of human milk when compared with bovine milk is still a debatable subject.
34130289	2	57	theme	exposure	233:240	arg1	effect					202:207	the effect	198:207	the effect of human or bovine milk exposure on biofilm composition and enamel demineralization	198:291	Therefore, we evaluated the effect of human or bovine milk exposure on biofilm composition and enamel demineralization using a validated cariogenic biofilm model.
34130289	5	58	theme	acidogenicity	768:780	arg1	indicator					747:755	an indicator	744:755	an indicator of biofilm acidogenicity	744:780	The culture medium was changed twice daily, and the pH was analyzed as an indicator of biofilm acidogenicity.
34130289	5	58	theme	acidogenicity	768:780	arg1	pH					725:726	the pH	721:726	the pH	721:726	The culture medium was changed twice daily, and the pH was analyzed as an indicator of biofilm acidogenicity.
34130289	4	59	theme	bovine	616:621	arg1	control					628:634	active bovine milk control	609:634	active bovine milk control	609:634	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	4	59	theme	bovine	616:621	arg1	lactose					600:606	4.5% lactose	595:606	4.5% lactose (active bovine milk control)	595:635	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	3	60	theme	surface	444:450	arg1	hardness					452:459	known surface hardness	438:459	known surface hardness	438:459	Streptococcus mutans UA159 biofilms (n = 8) were grown on human saliva-coated bovine enamel slabs of known surface hardness.
34130289	2	61	theme	bovine	221:226	arg1	exposure					233:240	human or bovine milk exposure	212:240	human or bovine milk exposure	212:240	Therefore, we evaluated the effect of human or bovine milk exposure on biofilm composition and enamel demineralization using a validated cariogenic biofilm model.
34130289	1	62	theme	bovine	132:137	arg1	milk					139:142	bovine milk	132:142	bovine milk	132:142	The higher cariogenicity of human milk when compared with bovine milk is still a debatable subject.
34130289	6	63	theme	viable	842:847	arg1	insoluble					872:880	insoluble	872:880	insoluble	872:880	After 120 h of growth, biofilms were harvested to evaluate viable cells, and soluble and insoluble extracellular polysaccharides (EPS).
34130289	6	63	theme	viable	842:847	arg1	cells					849:853	viable cells	842:853	viable cells	842:853	After 120 h of growth, biofilms were harvested to evaluate viable cells, and soluble and insoluble extracellular polysaccharides (EPS).
34130289	6	63	theme	viable	842:847	arg1	soluble					860:866	soluble	860:866	soluble	860:866	After 120 h of growth, biofilms were harvested to evaluate viable cells, and soluble and insoluble extracellular polysaccharides (EPS).
34130289	0	64	from	Potential	11:19	arg1	Demineralization					56:71	Enamel Demineralization	49:71	Enamel Demineralization	49:71	Cariogenic Potential of Human and Bovine Milk on Enamel Demineralization.
34130289	4	65	theme	%	598:598	arg1	control					628:634	active bovine milk control	609:634	active bovine milk control	609:634	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	4	65	theme	%	598:598	arg1	lactose					600:606	4.5% lactose	595:606	4.5% lactose (active bovine milk control)	595:635	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	9	66	theme	SHL	1079:1081	arg1	terms					1069:1073	terms	1069:1073	terms of %SHL	1069:1081	In terms of %SHL, negative control (7.7 ± 3.1), human milk control (13.3 ± 7.5), bovine milk control (15.3 ± 8.2), human milk (7.5 ± 5.0), and bovine milk (8.7 ± 6.3) did not differ among them (p > 0.05) but differed (p < 0.05) from sucrose (55.1 ± 5.4).
34130289	9	67	theme	bovine	1209:1214	arg1	milk					1216:1219	bovine milk	1209:1219	bovine milk (8.7 ± 6.3)	1209:1231	In terms of %SHL, negative control (7.7 ± 3.1), human milk control (13.3 ± 7.5), bovine milk control (15.3 ± 8.2), human milk (7.5 ± 5.0), and bovine milk (8.7 ± 6.3) did not differ among them (p > 0.05) but differed (p < 0.05) from sucrose (55.1 ± 5.4).
34130289	9	67	theme	bovine	1209:1214	arg1	±					1226:1226	8.7 ± 6.3	1222:1230	8.7 ± 6.3	1222:1230	In terms of %SHL, negative control (7.7 ± 3.1), human milk control (13.3 ± 7.5), bovine milk control (15.3 ± 8.2), human milk (7.5 ± 5.0), and bovine milk (8.7 ± 6.3) did not differ among them (p > 0.05) but differed (p < 0.05) from sucrose (55.1 ± 5.4).
34130289	2	68	theme	enamel	269:274	arg1	demineralization					276:291	enamel demineralization	269:291	enamel demineralization	269:291	Therefore, we evaluated the effect of human or bovine milk exposure on biofilm composition and enamel demineralization using a validated cariogenic biofilm model.
34130289	3	69	theme	UA159	358:362	arg1	n					374:374	n = 8	374:378	n = 8	374:378	Streptococcus mutans UA159 biofilms (n = 8) were grown on human saliva-coated bovine enamel slabs of known surface hardness.
34130289	3	69	theme	UA159	358:362	arg1	biofilms					364:371	Streptococcus mutans UA159 biofilms	337:371	Streptococcus mutans UA159 biofilms (n = 8)	337:379	Streptococcus mutans UA159 biofilms (n = 8) were grown on human saliva-coated bovine enamel slabs of known surface hardness.
34130289	4	70	theme	10	641:642	arg1	%					643:643	%	643:643	%	643:643	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	2	71	theme	validated	301:309	arg1	model					330:334	a validated cariogenic biofilm model	299:334	a validated cariogenic biofilm model	299:334	Therefore, we evaluated the effect of human or bovine milk exposure on biofilm composition and enamel demineralization using a validated cariogenic biofilm model.
34130289	9	72	theme	negative	1084:1091	arg1	control					1093:1099	negative control	1084:1099	negative control (7.7 ± 3.1)	1084:1111	In terms of %SHL, negative control (7.7 ± 3.1), human milk control (13.3 ± 7.5), bovine milk control (15.3 ± 8.2), human milk (7.5 ± 5.0), and bovine milk (8.7 ± 6.3) did not differ among them (p > 0.05) but differed (p < 0.05) from sucrose (55.1 ± 5.4).
34130289	9	72	theme	negative	1084:1091	arg1	±					1106:1106	7.7 ± 3.1	1102:1110	7.7 ± 3.1	1102:1110	In terms of %SHL, negative control (7.7 ± 3.1), human milk control (13.3 ± 7.5), bovine milk control (15.3 ± 8.2), human milk (7.5 ± 5.0), and bovine milk (8.7 ± 6.3) did not differ among them (p > 0.05) but differed (p < 0.05) from sucrose (55.1 ± 5.4).
34130289	6	73	dep	soluble	860:866	arg1	polysaccharides					896:910	extracellular polysaccharides	882:910	extracellular polysaccharides	882:910	After 120 h of growth, biofilms were harvested to evaluate viable cells, and soluble and insoluble extracellular polysaccharides (EPS).
34130289	3	74	theme	bovine	415:420	arg1	slabs					429:433	human saliva-coated bovine enamel slabs	395:433	human saliva-coated bovine enamel slabs of known surface hardness	395:459	Streptococcus mutans UA159 biofilms (n = 8) were grown on human saliva-coated bovine enamel slabs of known surface hardness.
34130289	9	75	dep	differed	1274:1281	arg1	<					1286:1286	p < 0.05	1284:1291	p < 0.05	1284:1291	In terms of %SHL, negative control (7.7 ± 3.1), human milk control (13.3 ± 7.5), bovine milk control (15.3 ± 8.2), human milk (7.5 ± 5.0), and bovine milk (8.7 ± 6.3) did not differ among them (p > 0.05) but differed (p < 0.05) from sucrose (55.1 ± 5.4).
34130289	1	76	theme	higher	78:83	arg1	subject					165:171	a debatable subject	153:171	a debatable subject	153:171	The higher cariogenicity of human milk when compared with bovine milk is still a debatable subject.
34130289	1	76	theme	higher	78:83	arg1	cariogenicity					85:97	The higher cariogenicity	74:97	The higher cariogenicity of human milk when compared with bovine milk	74:142	The higher cariogenicity of human milk when compared with bovine milk is still a debatable subject.
34130289	3	77	theme	=	376:376	arg1	n					374:374	n = 8	374:378	n = 8	374:378	Streptococcus mutans UA159 biofilms (n = 8) were grown on human saliva-coated bovine enamel slabs of known surface hardness.
34130289	3	77	theme	=	376:376	arg1	biofilms					364:371	Streptococcus mutans UA159 biofilms	337:371	Streptococcus mutans UA159 biofilms (n = 8)	337:379	Streptococcus mutans UA159 biofilms (n = 8) were grown on human saliva-coated bovine enamel slabs of known surface hardness.
34130289	4	78	theme	negative	509:516	arg1	NaCl					503:506	0.9% NaCl	498:506	0.9% NaCl (negative control)	498:525	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	4	78	theme	negative	509:516	arg1	control					518:524	negative control	509:524	negative control	509:524	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	3	79	theme	human	395:399	arg1	slabs					429:433	human saliva-coated bovine enamel slabs	395:433	human saliva-coated bovine enamel slabs of known surface hardness	395:459	Streptococcus mutans UA159 biofilms (n = 8) were grown on human saliva-coated bovine enamel slabs of known surface hardness.
34130289	0	80	theme	Human	24:28	arg1	Milk					41:44	Human and Bovine Milk	24:44	Human and Bovine Milk	24:44	Cariogenic Potential of Human and Bovine Milk on Enamel Demineralization.
34130289	10	81	theme	bacterial	1435:1443	arg1	counts					1445:1450	bacterial counts	1435:1450	bacterial counts	1435:1450	The findings of enamel demineralization (%SHL) were statistically supported by the data of biofilm acidogenicity, bacterial counts and EPS biofilm composition.
34130289	3	82	theme	known	438:442	arg1	hardness					452:459	known surface hardness	438:459	known surface hardness	438:459	Streptococcus mutans UA159 biofilms (n = 8) were grown on human saliva-coated bovine enamel slabs of known surface hardness.
34130289	7	83	theme	loss	994:997	arg1	percentage					963:972	the percentage	959:972	the percentage of surface hardness loss (%SHL)	959:1004	Enamel demineralization was assessed by the percentage of surface hardness loss (%SHL).
34130289	4	84	theme	0.9	498:500	arg1	%					501:501	%	501:501	%	501:501	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	10	85	theme	acidogenicity	1420:1432	arg1	data					1404:1407	the data	1400:1407	the data of biofilm acidogenicity, bacterial counts and EPS biofilm composition	1400:1478	The findings of enamel demineralization (%SHL) were statistically supported by the data of biofilm acidogenicity, bacterial counts and EPS biofilm composition.
34130289	7	86	theme	surface	977:983	arg1	loss					994:997	surface hardness loss	977:997	surface hardness loss (%SHL)	977:1004	Enamel demineralization was assessed by the percentage of surface hardness loss (%SHL).
34130289	7	86	theme	surface	977:983	arg1	SHL					1001:1003	%SHL	1000:1003	%SHL	1000:1003	Enamel demineralization was assessed by the percentage of surface hardness loss (%SHL).
34130289	9	87	theme	human	1114:1118	arg1	±					1139:1139	13.3 ± 7.5	1134:1143	13.3 ± 7.5	1134:1143	In terms of %SHL, negative control (7.7 ± 3.1), human milk control (13.3 ± 7.5), bovine milk control (15.3 ± 8.2), human milk (7.5 ± 5.0), and bovine milk (8.7 ± 6.3) did not differ among them (p > 0.05) but differed (p < 0.05) from sucrose (55.1 ± 5.4).
34130289	9	87	theme	human	1114:1118	arg1	control					1125:1131	human milk control	1114:1131	human milk control (13.3 ± 7.5)	1114:1144	In terms of %SHL, negative control (7.7 ± 3.1), human milk control (13.3 ± 7.5), bovine milk control (15.3 ± 8.2), human milk (7.5 ± 5.0), and bovine milk (8.7 ± 6.3) did not differ among them (p > 0.05) but differed (p < 0.05) from sucrose (55.1 ± 5.4).
34130289	10	88	theme	biofilm	1460:1466	arg1	composition					1468:1478	EPS biofilm composition	1456:1478	EPS biofilm composition	1456:1478	The findings of enamel demineralization (%SHL) were statistically supported by the data of biofilm acidogenicity, bacterial counts and EPS biofilm composition.
34130289	2	89	theme	biofilm	322:328	arg1	model					330:334	a validated cariogenic biofilm model	299:334	a validated cariogenic biofilm model	299:334	Therefore, we evaluated the effect of human or bovine milk exposure on biofilm composition and enamel demineralization using a validated cariogenic biofilm model.
34130289	4	90	theme	positive	654:661	arg1	sucrose					645:651	10% sucrose	641:651	10% sucrose (positive control)	641:670	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	4	90	theme	positive	654:661	arg1	control					663:669	positive control	654:669	positive control	654:669	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
34130289	3	91	theme	hardness	452:459	arg1	slabs					429:433	human saliva-coated bovine enamel slabs	395:433	human saliva-coated bovine enamel slabs of known surface hardness	395:459	Streptococcus mutans UA159 biofilms (n = 8) were grown on human saliva-coated bovine enamel slabs of known surface hardness.
34130289	11	92	theme	experimental	1486:1497	arg1	study					1499:1503	This experimental study	1481:1503	This experimental study	1481:1503	This experimental study suggests that human and bovine milk have low cariogenic potential to provoke caries lesions in enamel.
34130289	4	93	theme	7.0	553:555	arg1	%					556:556	%	556:556	%	556:556	The biofilms were exposed 8×/day to 0.9% NaCl (negative control), human milk, bovine milk, 7.0% lactose (active human milk control), 4.5% lactose (active bovine milk control), or 10% sucrose (positive control).
32497755	4	0	theme	model	879:883	arg1	studies					885:891	model studies	879:891	model studies	879:891	Besides, the recovery of these REEs was not affected by competitive alkali, alkaline earth, and transition metal ions in model studies and industrial wastewater as revealed by the recovery efficiencies, which ranged from 62% to 100% indicating an excellent selectivity on both adsorbents.
32497755	3	1	theme	Langmuir	606:613	arg1	model					624:628	the Langmuir isotherm model	602:628	the Langmuir isotherm model with maximum adsorption capacities ranging from 7.76 to 9.59 mg/g	602:694	Both P-CDP and P-CDP@Fe3O4 rapidly sequester REEs such as Nd, Gd, Eu, and Y, reaching equilibrium in less than 10 min and fitting the Langmuir isotherm model with maximum adsorption capacities ranging from 7.76 to 9.59 mg/g at 25 °C when the highest initial concentration was 100 mg/L.
32497755	1	2	from	demand	302:307	arg1	technologies					319:330	modern technologies	312:330	modern technologies	312:330	Recovery of rare earth elements (REEs) from industrial wastewater has drawn great attention due to their potential environmental toxicity, as well as their high demand in modern technologies.
32497755	1	3	theme	earth	158:162	arg1	REEs					174:177	REEs	174:177	REEs	174:177	Recovery of rare earth elements (REEs) from industrial wastewater has drawn great attention due to their potential environmental toxicity, as well as their high demand in modern technologies.
32497755	1	3	theme	earth	158:162	arg1	elements					164:171	rare earth elements	153:171	rare earth elements (REEs)	153:178	Recovery of rare earth elements (REEs) from industrial wastewater has drawn great attention due to their potential environmental toxicity, as well as their high demand in modern technologies.
32497755	4	4	theme	recovery	938:945	arg1	efficiencies					947:958	the recovery efficiencies	934:958	the recovery efficiencies	934:958	Besides, the recovery of these REEs was not affected by competitive alkali, alkaline earth, and transition metal ions in model studies and industrial wastewater as revealed by the recovery efficiencies, which ranged from 62% to 100% indicating an excellent selectivity on both adsorbents.
32497755	8	5	theme	@	1501:1501	arg1	Fe3O4					1502:1506	P-CDP@Fe3O4	1496:1506	P-CDP@Fe3O4	1496:1506	These characteristics demonstrate the promise of P-CDP and P-CDP@Fe3O4 for the pollution control and recovery of REEs.
32497755	4	6	theme	competitive	814:824	arg1	alkali					826:831	competitive alkali	814:831	competitive alkali	814:831	Besides, the recovery of these REEs was not affected by competitive alkali, alkaline earth, and transition metal ions in model studies and industrial wastewater as revealed by the recovery efficiencies, which ranged from 62% to 100% indicating an excellent selectivity on both adsorbents.
32497755	1	7	theme	elements	164:171	arg1	Recovery					141:148	Recovery	141:148	Recovery of rare earth elements (REEs) from industrial wastewater	141:205	Recovery of rare earth elements (REEs) from industrial wastewater has drawn great attention due to their potential environmental toxicity, as well as their high demand in modern technologies.
32497755	1	8	theme	potential	246:254	arg1	toxicity					270:277	their potential environmental toxicity	240:277	their potential environmental toxicity	240:277	Recovery of rare earth elements (REEs) from industrial wastewater has drawn great attention due to their potential environmental toxicity, as well as their high demand in modern technologies.
32497755	6	9	theme	magnetic	1230:1237	arg1	field					1239:1243	an external magnetic field	1218:1243	an external magnetic field which simplifies its synthesis and usability	1218:1288	Moreover, P-CDP@Fe3O4 can be easily isolated by an external magnetic field which simplifies its synthesis and usability.
32497755	8	10	dep	control	1526:1532	arg1	the					1512:1514	the	1512:1514	the	1512:1514	These characteristics demonstrate the promise of P-CDP and P-CDP@Fe3O4 for the pollution control and recovery of REEs.
32497755	4	11	theme	REEs	789:792	arg1	recovery					771:778	the recovery	767:778	the recovery of these REEs	767:792	Besides, the recovery of these REEs was not affected by competitive alkali, alkaline earth, and transition metal ions in model studies and industrial wastewater as revealed by the recovery efficiencies, which ranged from 62% to 100% indicating an excellent selectivity on both adsorbents.
32497755	3	12	theme	@	492:492	arg1	Fe3O4					493:497	P-CDP@Fe3O4	487:497	P-CDP@Fe3O4	487:497	Both P-CDP and P-CDP@Fe3O4 rapidly sequester REEs such as Nd, Gd, Eu, and Y, reaching equilibrium in less than 10 min and fitting the Langmuir isotherm model with maximum adsorption capacities ranging from 7.76 to 9.59 mg/g at 25 °C when the highest initial concentration was 100 mg/L.
32497755	8	13	theme	REEs	1550:1553	arg1	control					1526:1532	pollution control	1516:1532	pollution control	1516:1532	These characteristics demonstrate the promise of P-CDP and P-CDP@Fe3O4 for the pollution control and recovery of REEs.
32497755	8	13	theme	REEs	1550:1553	arg1	recovery					1538:1545	recovery	1538:1545	recovery	1538:1545	These characteristics demonstrate the promise of P-CDP and P-CDP@Fe3O4 for the pollution control and recovery of REEs.
32497755	1	14	theme	environmental	256:268	arg1	toxicity					270:277	their potential environmental toxicity	240:277	their potential environmental toxicity	240:277	Recovery of rare earth elements (REEs) from industrial wastewater has drawn great attention due to their potential environmental toxicity, as well as their high demand in modern technologies.
32497755	0	15	theme	porous	81:86	arg1	β-cyclodextrin					88:101	porous β-cyclodextrin	81:101	porous β-cyclodextrin	81:101	Selective and fast recovery of rare earth elements from industrial wastewater by porous β-cyclodextrin and magnetic β-cyclodextrin polymers.
32497755	7	16	theme	REEs	1399:1402	arg1	recovery					1404:1411	REEs recovery	1399:1411	REEs recovery	1399:1411	It also overcomes the clogging and high backpressure issues of P-CDP, which facilitates its application for REEs recovery as compared with P-CDP.
32497755	7	17	theme	P-CDP	1354:1358	arg1	issues					1344:1349	the clogging and high backpressure issues	1309:1349	the clogging and high backpressure issues	1309:1349	It also overcomes the clogging and high backpressure issues of P-CDP, which facilitates its application for REEs recovery as compared with P-CDP.
32497755	4	18	theme	excellent	1005:1013	arg1	selectivity					1015:1025	an excellent selectivity	1002:1025	an excellent selectivity on both adsorbents	1002:1044	Besides, the recovery of these REEs was not affected by competitive alkali, alkaline earth, and transition metal ions in model studies and industrial wastewater as revealed by the recovery efficiencies, which ranged from 62% to 100% indicating an excellent selectivity on both adsorbents.
32497755	8	19	theme	Fe3O4	1502:1506	arg1	promise					1475:1481	the promise	1471:1481	the promise of P-CDP and P-CDP@Fe3O4 for the pollution control and recovery of REEs	1471:1553	These characteristics demonstrate the promise of P-CDP and P-CDP@Fe3O4 for the pollution control and recovery of REEs.
32497755	0	20	theme	magnetic	107:114	arg1	polymers					131:138	magnetic β-cyclodextrin polymers	107:138	magnetic β-cyclodextrin polymers	107:138	Selective and fast recovery of rare earth elements from industrial wastewater by porous β-cyclodextrin and magnetic β-cyclodextrin polymers.
32497755	3	21	theme	maximum	635:641	arg1	capacities					654:663	maximum adsorption capacities	635:663	maximum adsorption capacities ranging from 7.76 to 9.59 mg/g	635:694	Both P-CDP and P-CDP@Fe3O4 rapidly sequester REEs such as Nd, Gd, Eu, and Y, reaching equilibrium in less than 10 min and fitting the Langmuir isotherm model with maximum adsorption capacities ranging from 7.76 to 9.59 mg/g at 25 °C when the highest initial concentration was 100 mg/L.
32497755	3	22	theme	initial	722:728	arg1	concentration					730:742	the highest initial concentration	710:742	the highest initial concentration	710:742	Both P-CDP and P-CDP@Fe3O4 rapidly sequester REEs such as Nd, Gd, Eu, and Y, reaching equilibrium in less than 10 min and fitting the Langmuir isotherm model with maximum adsorption capacities ranging from 7.76 to 9.59 mg/g at 25 °C when the highest initial concentration was 100 mg/L.
32497755	3	22	theme	initial	722:728	arg1	100 mg/L					748:755	100 mg/L	748:755	100 mg/L	748:755	Both P-CDP and P-CDP@Fe3O4 rapidly sequester REEs such as Nd, Gd, Eu, and Y, reaching equilibrium in less than 10 min and fitting the Langmuir isotherm model with maximum adsorption capacities ranging from 7.76 to 9.59 mg/g at 25 °C when the highest initial concentration was 100 mg/L.
32497755	4	23	theme	industrial	897:906	arg1	wastewater					908:917	industrial wastewater	897:917	industrial wastewater	897:917	Besides, the recovery of these REEs was not affected by competitive alkali, alkaline earth, and transition metal ions in model studies and industrial wastewater as revealed by the recovery efficiencies, which ranged from 62% to 100% indicating an excellent selectivity on both adsorbents.
32497755	3	24	theme	isotherm	615:622	arg1	model					624:628	the Langmuir isotherm model	602:628	the Langmuir isotherm model with maximum adsorption capacities ranging from 7.76 to 9.59 mg/g	602:694	Both P-CDP and P-CDP@Fe3O4 rapidly sequester REEs such as Nd, Gd, Eu, and Y, reaching equilibrium in less than 10 min and fitting the Langmuir isotherm model with maximum adsorption capacities ranging from 7.76 to 9.59 mg/g at 25 °C when the highest initial concentration was 100 mg/L.
32497755	4	25	dep	%	981:981	arg1	to					983:984	to	983:984	to	983:984	Besides, the recovery of these REEs was not affected by competitive alkali, alkaline earth, and transition metal ions in model studies and industrial wastewater as revealed by the recovery efficiencies, which ranged from 62% to 100% indicating an excellent selectivity on both adsorbents.
32497755	2	26	theme	porous	413:418	arg1	P-CDP					444:448	P-CDP	444:448	P-CDP	444:448	In this study, we developed a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4.
32497755	2	26	theme	porous	413:418	arg1	Fe3O4					465:469	P-CDP@Fe3O4	459:469	P-CDP@Fe3O4	459:469	In this study, we developed a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4.
32497755	2	26	theme	porous	413:418	arg1	polymer					435:441	the high surface area porous β-cyclodextrin polymer	391:441	the high surface area porous β-cyclodextrin polymer (P-CDP)	391:449	In this study, we developed a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4.
32497755	2	27	theme	magnetic	363:370	arg1	composite					372:380	a magnetic composite	361:380	a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4	361:469	In this study, we developed a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4.
32497755	2	28	theme	β-cyclodextrin	420:433	arg1	P-CDP					444:448	P-CDP	444:448	P-CDP	444:448	In this study, we developed a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4.
32497755	2	28	theme	β-cyclodextrin	420:433	arg1	Fe3O4					465:469	P-CDP@Fe3O4	459:469	P-CDP@Fe3O4	459:469	In this study, we developed a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4.
32497755	2	28	theme	β-cyclodextrin	420:433	arg1	polymer					435:441	the high surface area porous β-cyclodextrin polymer	391:441	the high surface area porous β-cyclodextrin polymer (P-CDP)	391:449	In this study, we developed a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4.
32497755	3	29	theme	P-CDP	487:491	arg1	Fe3O4					493:497	P-CDP@Fe3O4	487:497	P-CDP@Fe3O4	487:497	Both P-CDP and P-CDP@Fe3O4 rapidly sequester REEs such as Nd, Gd, Eu, and Y, reaching equilibrium in less than 10 min and fitting the Langmuir isotherm model with maximum adsorption capacities ranging from 7.76 to 9.59 mg/g at 25 °C when the highest initial concentration was 100 mg/L.
32497755	2	30	theme	@	464:464	arg1	polymer					435:441	the high surface area porous β-cyclodextrin polymer	391:441	the high surface area porous β-cyclodextrin polymer (P-CDP)	391:449	In this study, we developed a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4.
32497755	2	30	theme	@	464:464	arg1	Fe3O4					465:469	P-CDP@Fe3O4	459:469	P-CDP@Fe3O4	459:469	In this study, we developed a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4.
32497755	6	31	theme	@	1185:1185	arg1	Fe3O4					1186:1190	P-CDP@Fe3O4	1180:1190	P-CDP@Fe3O4	1180:1190	Moreover, P-CDP@Fe3O4 can be easily isolated by an external magnetic field which simplifies its synthesis and usability.
32497755	0	32	theme	Selective	0:8	arg1	recovery					19:26	Selective and fast recovery	0:26	Selective and fast recovery of rare earth elements from industrial wastewater by porous β-cyclodextrin and magnetic β-cyclodextrin polymers.	0:139	Selective and fast recovery of rare earth elements from industrial wastewater by porous β-cyclodextrin and magnetic β-cyclodextrin polymers.
32497755	7	33	theme	backpressure	1331:1342	arg1	issues					1344:1349	the clogging and high backpressure issues	1309:1349	the clogging and high backpressure issues	1309:1349	It also overcomes the clogging and high backpressure issues of P-CDP, which facilitates its application for REEs recovery as compared with P-CDP.
32497755	4	34	from	selectivity	1015:1025	arg1	adsorbents					1035:1044	both adsorbents	1030:1044	both adsorbents	1030:1044	Besides, the recovery of these REEs was not affected by competitive alkali, alkaline earth, and transition metal ions in model studies and industrial wastewater as revealed by the recovery efficiencies, which ranged from 62% to 100% indicating an excellent selectivity on both adsorbents.
32497755	4	35	theme	100	986:988	arg1	%					981:981	%	981:981	%	981:981	Besides, the recovery of these REEs was not affected by competitive alkali, alkaline earth, and transition metal ions in model studies and industrial wastewater as revealed by the recovery efficiencies, which ranged from 62% to 100% indicating an excellent selectivity on both adsorbents.
32497755	2	36	theme	area	408:411	arg1	P-CDP					444:448	P-CDP	444:448	P-CDP	444:448	In this study, we developed a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4.
32497755	2	36	theme	area	408:411	arg1	Fe3O4					465:469	P-CDP@Fe3O4	459:469	P-CDP@Fe3O4	459:469	In this study, we developed a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4.
32497755	2	36	theme	area	408:411	arg1	polymer					435:441	the high surface area porous β-cyclodextrin polymer	391:441	the high surface area porous β-cyclodextrin polymer (P-CDP)	391:449	In this study, we developed a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4.
32497755	1	37	theme	industrial	185:194	arg1	wastewater					196:205	industrial wastewater	185:205	industrial wastewater	185:205	Recovery of rare earth elements (REEs) from industrial wastewater has drawn great attention due to their potential environmental toxicity, as well as their high demand in modern technologies.
32497755	0	38	theme	fast	14:17	arg1	recovery					19:26	Selective and fast recovery	0:26	Selective and fast recovery of rare earth elements from industrial wastewater by porous β-cyclodextrin and magnetic β-cyclodextrin polymers.	0:139	Selective and fast recovery of rare earth elements from industrial wastewater by porous β-cyclodextrin and magnetic β-cyclodextrin polymers.
32497755	3	39	theme	adsorption	643:652	arg1	capacities					654:663	maximum adsorption capacities	635:663	maximum adsorption capacities ranging from 7.76 to 9.59 mg/g	635:694	Both P-CDP and P-CDP@Fe3O4 rapidly sequester REEs such as Nd, Gd, Eu, and Y, reaching equilibrium in less than 10 min and fitting the Langmuir isotherm model with maximum adsorption capacities ranging from 7.76 to 9.59 mg/g at 25 °C when the highest initial concentration was 100 mg/L.
32497755	0	40	theme	β-cyclodextrin	116:129	arg1	polymers					131:138	magnetic β-cyclodextrin polymers	107:138	magnetic β-cyclodextrin polymers	107:138	Selective and fast recovery of rare earth elements from industrial wastewater by porous β-cyclodextrin and magnetic β-cyclodextrin polymers.
32497755	0	41	theme	rare	31:34	arg1	elements					42:49	rare earth elements	31:49	rare earth elements from industrial wastewater	31:76	Selective and fast recovery of rare earth elements from industrial wastewater by porous β-cyclodextrin and magnetic β-cyclodextrin polymers.
32497755	4	42	theme	alkaline	834:841	arg1	earth					843:847	alkaline earth	834:847	alkaline earth	834:847	Besides, the recovery of these REEs was not affected by competitive alkali, alkaline earth, and transition metal ions in model studies and industrial wastewater as revealed by the recovery efficiencies, which ranged from 62% to 100% indicating an excellent selectivity on both adsorbents.
32497755	0	43	from	wastewater	67:76	arg1	recovery					19:26	Selective and fast recovery	0:26	Selective and fast recovery of rare earth elements from industrial wastewater by porous β-cyclodextrin and magnetic β-cyclodextrin polymers.	0:139	Selective and fast recovery of rare earth elements from industrial wastewater by porous β-cyclodextrin and magnetic β-cyclodextrin polymers.
32497755	0	43	from	wastewater	67:76	arg1	elements					42:49	rare earth elements	31:49	rare earth elements from industrial wastewater	31:76	Selective and fast recovery of rare earth elements from industrial wastewater by porous β-cyclodextrin and magnetic β-cyclodextrin polymers.
32497755	8	44	theme	P-CDP	1486:1490	arg1	promise					1475:1481	the promise	1471:1481	the promise of P-CDP and P-CDP@Fe3O4 for the pollution control and recovery of REEs	1471:1553	These characteristics demonstrate the promise of P-CDP and P-CDP@Fe3O4 for the pollution control and recovery of REEs.
32497755	5	45	theme	consecutive	1150:1160	arg1	cycles					1162:1167	at least five consecutive cycles	1136:1167	at least five consecutive cycles	1136:1167	In addition, both adsorbents can be fully regenerated under mildly acidic conditions for at least five consecutive cycles.
32497755	8	46	theme	P-CDP	1496:1500	arg1	Fe3O4					1502:1506	P-CDP@Fe3O4	1496:1506	P-CDP@Fe3O4	1496:1506	These characteristics demonstrate the promise of P-CDP and P-CDP@Fe3O4 for the pollution control and recovery of REEs.
32497755	8	47	theme	pollution	1516:1524	arg1	control					1526:1532	pollution control	1516:1532	pollution control	1516:1532	These characteristics demonstrate the promise of P-CDP and P-CDP@Fe3O4 for the pollution control and recovery of REEs.
32497755	0	48	theme	elements	42:49	arg1	recovery					19:26	Selective and fast recovery	0:26	Selective and fast recovery of rare earth elements from industrial wastewater by porous β-cyclodextrin and magnetic β-cyclodextrin polymers.	0:139	Selective and fast recovery of rare earth elements from industrial wastewater by porous β-cyclodextrin and magnetic β-cyclodextrin polymers.
32497755	1	49	theme	high	297:300	arg1	demand					302:307	their high demand	291:307	their high demand in modern technologies	291:330	Recovery of rare earth elements (REEs) from industrial wastewater has drawn great attention due to their potential environmental toxicity, as well as their high demand in modern technologies.
32497755	7	50	theme	high	1326:1329	arg1	backpressure					1331:1342	high backpressure	1326:1342	high backpressure	1326:1342	It also overcomes the clogging and high backpressure issues of P-CDP, which facilitates its application for REEs recovery as compared with P-CDP.
32497755	4	51	theme	metal	865:869	arg1	ions					871:874	transition metal ions	854:874	transition metal ions	854:874	Besides, the recovery of these REEs was not affected by competitive alkali, alkaline earth, and transition metal ions in model studies and industrial wastewater as revealed by the recovery efficiencies, which ranged from 62% to 100% indicating an excellent selectivity on both adsorbents.
32497755	7	52	theme	clogging	1313:1320	arg1	issues					1344:1349	the clogging and high backpressure issues	1309:1349	the clogging and high backpressure issues	1309:1349	It also overcomes the clogging and high backpressure issues of P-CDP, which facilitates its application for REEs recovery as compared with P-CDP.
32497755	0	53	theme	earth	36:40	arg1	elements					42:49	rare earth elements	31:49	rare earth elements from industrial wastewater	31:76	Selective and fast recovery of rare earth elements from industrial wastewater by porous β-cyclodextrin and magnetic β-cyclodextrin polymers.
32497755	1	54	theme	modern	312:317	arg1	technologies					319:330	modern technologies	312:330	modern technologies	312:330	Recovery of rare earth elements (REEs) from industrial wastewater has drawn great attention due to their potential environmental toxicity, as well as their high demand in modern technologies.
32497755	4	55	theme	transition	854:863	arg1	ions					871:874	transition metal ions	854:874	transition metal ions	854:874	Besides, the recovery of these REEs was not affected by competitive alkali, alkaline earth, and transition metal ions in model studies and industrial wastewater as revealed by the recovery efficiencies, which ranged from 62% to 100% indicating an excellent selectivity on both adsorbents.
32497755	6	56	theme	P-CDP	1180:1184	arg1	Fe3O4					1186:1190	P-CDP@Fe3O4	1180:1190	P-CDP@Fe3O4	1180:1190	Moreover, P-CDP@Fe3O4 can be easily isolated by an external magnetic field which simplifies its synthesis and usability.
32497755	1	57	from	wastewater	196:205	arg1	Recovery					141:148	Recovery	141:148	Recovery of rare earth elements (REEs) from industrial wastewater	141:205	Recovery of rare earth elements (REEs) from industrial wastewater has drawn great attention due to their potential environmental toxicity, as well as their high demand in modern technologies.
32497755	3	58	with	model	624:628	arg1	capacities					654:663	maximum adsorption capacities	635:663	maximum adsorption capacities ranging from 7.76 to 9.59 mg/g	635:694	Both P-CDP and P-CDP@Fe3O4 rapidly sequester REEs such as Nd, Gd, Eu, and Y, reaching equilibrium in less than 10 min and fitting the Langmuir isotherm model with maximum adsorption capacities ranging from 7.76 to 9.59 mg/g at 25 °C when the highest initial concentration was 100 mg/L.
32497755	1	59	theme	great	217:221	arg1	attention					223:231	great attention	217:231	great attention due to their potential environmental toxicity	217:277	Recovery of rare earth elements (REEs) from industrial wastewater has drawn great attention due to their potential environmental toxicity, as well as their high demand in modern technologies.
32497755	1	60	from	attention	223:231	arg1	technologies					319:330	modern technologies	312:330	modern technologies	312:330	Recovery of rare earth elements (REEs) from industrial wastewater has drawn great attention due to their potential environmental toxicity, as well as their high demand in modern technologies.
32497755	3	61	dep	sequester	507:515	arg1	reaching					549:556	reaching	549:556	reaching equilibrium in less than 10 min	549:588	Both P-CDP and P-CDP@Fe3O4 rapidly sequester REEs such as Nd, Gd, Eu, and Y, reaching equilibrium in less than 10 min and fitting the Langmuir isotherm model with maximum adsorption capacities ranging from 7.76 to 9.59 mg/g at 25 °C when the highest initial concentration was 100 mg/L.
32497755	3	61	dep	sequester	507:515	arg1	fitting					594:600	fitting	594:600	fitting the Langmuir isotherm model with maximum adsorption capacities ranging from 7.76 to 9.59 mg/g at 25 °C when the highest initial concentration was 100 mg/L	594:755	Both P-CDP and P-CDP@Fe3O4 rapidly sequester REEs such as Nd, Gd, Eu, and Y, reaching equilibrium in less than 10 min and fitting the Langmuir isotherm model with maximum adsorption capacities ranging from 7.76 to 9.59 mg/g at 25 °C when the highest initial concentration was 100 mg/L.
32497755	3	62	theme	highest	714:720	arg1	concentration					730:742	the highest initial concentration	710:742	the highest initial concentration	710:742	Both P-CDP and P-CDP@Fe3O4 rapidly sequester REEs such as Nd, Gd, Eu, and Y, reaching equilibrium in less than 10 min and fitting the Langmuir isotherm model with maximum adsorption capacities ranging from 7.76 to 9.59 mg/g at 25 °C when the highest initial concentration was 100 mg/L.
32497755	3	62	theme	highest	714:720	arg1	100 mg/L					748:755	100 mg/L	748:755	100 mg/L	748:755	Both P-CDP and P-CDP@Fe3O4 rapidly sequester REEs such as Nd, Gd, Eu, and Y, reaching equilibrium in less than 10 min and fitting the Langmuir isotherm model with maximum adsorption capacities ranging from 7.76 to 9.59 mg/g at 25 °C when the highest initial concentration was 100 mg/L.
32497755	0	63	theme	industrial	56:65	arg1	wastewater					67:76	industrial wastewater	56:76	industrial wastewater	56:76	Selective and fast recovery of rare earth elements from industrial wastewater by porous β-cyclodextrin and magnetic β-cyclodextrin polymers.
32497755	2	64	theme	high	395:398	arg1	P-CDP					444:448	P-CDP	444:448	P-CDP	444:448	In this study, we developed a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4.
32497755	2	64	theme	high	395:398	arg1	Fe3O4					465:469	P-CDP@Fe3O4	459:469	P-CDP@Fe3O4	459:469	In this study, we developed a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4.
32497755	2	64	theme	high	395:398	arg1	polymer					435:441	the high surface area porous β-cyclodextrin polymer	391:441	the high surface area porous β-cyclodextrin polymer (P-CDP)	391:449	In this study, we developed a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4.
32497755	4	65	theme	62	979:980	arg1	%					981:981	%	981:981	%	981:981	Besides, the recovery of these REEs was not affected by competitive alkali, alkaline earth, and transition metal ions in model studies and industrial wastewater as revealed by the recovery efficiencies, which ranged from 62% to 100% indicating an excellent selectivity on both adsorbents.
32497755	5	66	theme	acidic	1114:1119	arg1	conditions					1121:1130	mildly acidic conditions	1107:1130	mildly acidic conditions	1107:1130	In addition, both adsorbents can be fully regenerated under mildly acidic conditions for at least five consecutive cycles.
32497755	2	67	theme	P-CDP	459:463	arg1	polymer					435:441	the high surface area porous β-cyclodextrin polymer	391:441	the high surface area porous β-cyclodextrin polymer (P-CDP)	391:449	In this study, we developed a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4.
32497755	2	67	theme	P-CDP	459:463	arg1	Fe3O4					465:469	P-CDP@Fe3O4	459:469	P-CDP@Fe3O4	459:469	In this study, we developed a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4.
32497755	0	68	from	recovery	19:26	arg1	wastewater					67:76	industrial wastewater	56:76	industrial wastewater	56:76	Selective and fast recovery of rare earth elements from industrial wastewater by porous β-cyclodextrin and magnetic β-cyclodextrin polymers.
32497755	2	69	theme	surface	400:406	arg1	P-CDP					444:448	P-CDP	444:448	P-CDP	444:448	In this study, we developed a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4.
32497755	2	69	theme	surface	400:406	arg1	Fe3O4					465:469	P-CDP@Fe3O4	459:469	P-CDP@Fe3O4	459:469	In this study, we developed a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4.
32497755	2	69	theme	surface	400:406	arg1	polymer					435:441	the high surface area porous β-cyclodextrin polymer	391:441	the high surface area porous β-cyclodextrin polymer (P-CDP)	391:449	In this study, we developed a magnetic composite based on the high surface area porous β-cyclodextrin polymer (P-CDP), namely P-CDP@Fe3O4.
32497755	1	70	theme	rare	153:156	arg1	REEs					174:177	REEs	174:177	REEs	174:177	Recovery of rare earth elements (REEs) from industrial wastewater has drawn great attention due to their potential environmental toxicity, as well as their high demand in modern technologies.
32497755	1	70	theme	rare	153:156	arg1	elements					164:171	rare earth elements	153:171	rare earth elements (REEs)	153:178	Recovery of rare earth elements (REEs) from industrial wastewater has drawn great attention due to their potential environmental toxicity, as well as their high demand in modern technologies.
32497755	6	71	theme	external	1221:1228	arg1	field					1239:1243	an external magnetic field	1218:1243	an external magnetic field which simplifies its synthesis and usability	1218:1288	Moreover, P-CDP@Fe3O4 can be easily isolated by an external magnetic field which simplifies its synthesis and usability.
32175650	0	0	theme	macromolecules	100:113	arg1	degradation					64:74	spatially oriented degradation	45:74	spatially oriented degradation	45:74	Composite nanocellulose-based hydrogels with spatially oriented degradation and retarded release of macromolecules.
32175650	0	0	theme	macromolecules	100:113	arg1	release					89:95	retarded release	80:95	retarded release of macromolecules	80:113	Composite nanocellulose-based hydrogels with spatially oriented degradation and retarded release of macromolecules.
32175650	5	1	theme	long-term	1086:1094	arg1	release					1104:1110	the long-term protein release	1082:1110	the long-term protein release	1082:1110	The cup-like, scarcely interconnected porous network, with a gradient of hardness along the cylinder axis, and the compact skin-like layer covering the lateral wall which stayed in contact with the mold during gelification, explain the preferred erosion direction and the long-term protein release.
32175650	6	2	theme	surface	1241:1247	arg1	compaction					1249:1258	surface compaction	1241:1258	surface compaction	1241:1258	The possible effect of the molding process on hydrogel structure suggests that molding could be a simple and cheap way to favor surface compaction and directional scaffold degradation.
32175650	5	3	theme	hardness	887:894	arg1	gradient					875:882	a gradient	873:882	a gradient of hardness along the cylinder axis	873:918	The cup-like, scarcely interconnected porous network, with a gradient of hardness along the cylinder axis, and the compact skin-like layer covering the lateral wall which stayed in contact with the mold during gelification, explain the preferred erosion direction and the long-term protein release.
32175650	5	4	theme	protein	1096:1102	arg1	release					1104:1110	the long-term protein release	1082:1110	the long-term protein release	1082:1110	The cup-like, scarcely interconnected porous network, with a gradient of hardness along the cylinder axis, and the compact skin-like layer covering the lateral wall which stayed in contact with the mold during gelification, explain the preferred erosion direction and the long-term protein release.
32175650	4	5	theme	protein	588:594	arg1	release					548:554	The release	544:554	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel	544:643	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	4	5	theme	protein	588:594	arg1	meanwhile					684:692	meanwhile	684:692	meanwhile	684:692	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	4	5	theme	protein	588:594	arg1	slow					648:651	slow	648:651	slow	648:651	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	4	6	dep	cylinder	770:777	arg1	eroded					782:787	eroded	782:787	is eroded along the vertical axis	779:811	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	2	7	theme	cylinders	306:314	arg1	molding					276:282	molding	276:282	molding of composite hydrogel cylinders made of cellulose nanocrystals (CNCs) and chitosan (Cht)	276:371	Here, we report on fabrication by molding of composite hydrogel cylinders made of cellulose nanocrystals (CNCs) and chitosan (Cht) and their performance as delivery vehicles.
32175650	4	8	theme	serum	604:608	arg1	protein					588:594	a representative medium size protein	559:594	a representative medium size protein (bovine serum albumin) dispersed in the hydrogel	559:643	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	4	8	theme	serum	604:608	arg1	albumin					610:616	bovine serum albumin	597:616	bovine serum albumin	597:616	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	5	9	with	contact	995:1001	arg1	mold					1012:1015	the mold	1008:1015	the mold	1008:1015	The cup-like, scarcely interconnected porous network, with a gradient of hardness along the cylinder axis, and the compact skin-like layer covering the lateral wall which stayed in contact with the mold during gelification, explain the preferred erosion direction and the long-term protein release.
32175650	5	10	theme	preferred	1050:1058	arg1	direction					1068:1076	the preferred erosion direction	1046:1076	the preferred erosion direction	1046:1076	The cup-like, scarcely interconnected porous network, with a gradient of hardness along the cylinder axis, and the compact skin-like layer covering the lateral wall which stayed in contact with the mold during gelification, explain the preferred erosion direction and the long-term protein release.
32175650	5	11	theme	compact	929:935	arg1	layer					947:951	the compact skin-like layer	925:951	the compact skin-like layer covering the lateral wall which stayed in contact with the mold during gelification	925:1035	The cup-like, scarcely interconnected porous network, with a gradient of hardness along the cylinder axis, and the compact skin-like layer covering the lateral wall which stayed in contact with the mold during gelification, explain the preferred erosion direction and the long-term protein release.
32175650	2	12	theme	hydrogel	297:304	arg1	cylinders					306:314	composite hydrogel cylinders	287:314	composite hydrogel cylinders made of cellulose nanocrystals (CNCs) and chitosan (Cht)	287:371	Here, we report on fabrication by molding of composite hydrogel cylinders made of cellulose nanocrystals (CNCs) and chitosan (Cht) and their performance as delivery vehicles.
32175650	4	13	theme	bovine	597:602	arg1	protein					588:594	a representative medium size protein	559:594	a representative medium size protein (bovine serum albumin) dispersed in the hydrogel	559:643	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	4	13	theme	bovine	597:602	arg1	albumin					610:616	bovine serum albumin	597:616	bovine serum albumin	597:616	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	5	14	theme	erosion	1060:1066	arg1	direction					1068:1076	the preferred erosion direction	1046:1076	the preferred erosion direction	1046:1076	The cup-like, scarcely interconnected porous network, with a gradient of hardness along the cylinder axis, and the compact skin-like layer covering the lateral wall which stayed in contact with the mold during gelification, explain the preferred erosion direction and the long-term protein release.
32175650	5	15	with	layer	947:951	arg1	gradient					875:882	a gradient	873:882	a gradient of hardness along the cylinder axis	873:918	The cup-like, scarcely interconnected porous network, with a gradient of hardness along the cylinder axis, and the compact skin-like layer covering the lateral wall which stayed in contact with the mold during gelification, explain the preferred erosion direction and the long-term protein release.
32175650	2	16	theme	composite	287:295	arg1	cylinders					306:314	composite hydrogel cylinders	287:314	composite hydrogel cylinders made of cellulose nanocrystals (CNCs) and chitosan (Cht)	287:371	Here, we report on fabrication by molding of composite hydrogel cylinders made of cellulose nanocrystals (CNCs) and chitosan (Cht) and their performance as delivery vehicles.
32175650	4	17	theme	sigmoidal	665:673	arg1	profile					675:681	a sigmoidal profile	663:681	a sigmoidal profile	663:681	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	1	18	theme	novel	198:202	arg1	formulations					228:239	novel, robust, and controlled formulations	198:239	novel, robust, and controlled formulations	198:239	The oral delivery of macromolecular therapeutics to the intestinal tract requires novel, robust, and controlled formulations.
32175650	4	19	theme	medium	576:581	arg1	protein					588:594	a representative medium size protein	559:594	a representative medium size protein (bovine serum albumin) dispersed in the hydrogel	559:643	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	4	19	theme	medium	576:581	arg1	albumin					610:616	bovine serum albumin	597:616	bovine serum albumin	597:616	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	6	20	theme	simple	1211:1216	arg1	molding					1192:1198	molding	1192:1198	molding	1192:1198	The possible effect of the molding process on hydrogel structure suggests that molding could be a simple and cheap way to favor surface compaction and directional scaffold degradation.
32175650	6	20	theme	simple	1211:1216	arg1	way					1228:1230	a simple and cheap way	1209:1230	a simple and cheap way to favor surface compaction and directional scaffold degradation	1209:1295	The possible effect of the molding process on hydrogel structure suggests that molding could be a simple and cheap way to favor surface compaction and directional scaffold degradation.
32175650	5	21	theme	cylinder	906:913	arg1	axis					915:918	the cylinder axis	902:918	the cylinder axis	902:918	The cup-like, scarcely interconnected porous network, with a gradient of hardness along the cylinder axis, and the compact skin-like layer covering the lateral wall which stayed in contact with the mold during gelification, explain the preferred erosion direction and the long-term protein release.
32175650	6	22	theme	directional	1264:1274	arg1	degradation					1285:1295	directional scaffold degradation	1264:1295	directional scaffold degradation	1264:1295	The possible effect of the molding process on hydrogel structure suggests that molding could be a simple and cheap way to favor surface compaction and directional scaffold degradation.
32175650	1	23	theme	robust	205:210	arg1	formulations					228:239	novel, robust, and controlled formulations	198:239	novel, robust, and controlled formulations	198:239	The oral delivery of macromolecular therapeutics to the intestinal tract requires novel, robust, and controlled formulations.
32175650	0	24	theme	Composite	0:8	arg1	nanocellulose-based					10:28	Composite nanocellulose-based	0:28	Composite nanocellulose-based	0:28	Composite nanocellulose-based hydrogels with spatially oriented degradation and retarded release of macromolecules.
32175650	4	25	theme	representative	561:574	arg1	protein					588:594	a representative medium size protein	559:594	a representative medium size protein (bovine serum albumin) dispersed in the hydrogel	559:643	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	4	25	theme	representative	561:574	arg1	albumin					610:616	bovine serum albumin	597:616	bovine serum albumin	597:616	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	2	26	theme	cellulose	324:332	arg1	CNCs					348:351	CNCs	348:351	CNCs	348:351	Here, we report on fabrication by molding of composite hydrogel cylinders made of cellulose nanocrystals (CNCs) and chitosan (Cht) and their performance as delivery vehicles.
32175650	2	26	theme	cellulose	324:332	arg1	nanocrystals					334:345	cellulose nanocrystals	324:345	cellulose nanocrystals (CNCs)	324:352	Here, we report on fabrication by molding of composite hydrogel cylinders made of cellulose nanocrystals (CNCs) and chitosan (Cht) and their performance as delivery vehicles.
32175650	3	27	theme	excellent	430:438	arg1	resistance					471:480	excellent mechanical and chemical stress resistance	430:480	excellent mechanical and chemical stress resistance	430:480	CNCs provide excellent mechanical and chemical stress resistance, whereas Cht allows scaffold degradation by enzyme digestion.
32175650	6	28	theme	hydrogel	1159:1166	arg1	structure					1168:1176	hydrogel structure	1159:1176	hydrogel structure	1159:1176	The possible effect of the molding process on hydrogel structure suggests that molding could be a simple and cheap way to favor surface compaction and directional scaffold degradation.
32175650	1	29	theme	oral	120:123	arg1	delivery					125:132	The oral delivery	116:132	The oral delivery of macromolecular therapeutics to the intestinal tract	116:187	The oral delivery of macromolecular therapeutics to the intestinal tract requires novel, robust, and controlled formulations.
32175650	4	30	dep	slow	648:651	arg1	meanwhile					684:692	meanwhile	684:692	meanwhile	684:692	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	4	30	dep	slow	648:651	arg1	release					548:554	The release	544:554	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel	544:643	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	4	30	dep	slow	648:651	arg1	slow					648:651	slow	648:651	slow	648:651	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	4	30	dep	slow	648:651	arg1	scaffold					708:715	the hydrogel scaffold	695:715	the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis	695:811	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	4	31	dep	scaffold	708:715	arg1	degrades					717:724	degrades	717:724	degrades according to a preferred route, that is the cylinder is eroded along the vertical axis	717:811	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	4	32	theme	size	583:586	arg1	protein					588:594	a representative medium size protein	559:594	a representative medium size protein (bovine serum albumin) dispersed in the hydrogel	559:643	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	4	32	theme	size	583:586	arg1	albumin					610:616	bovine serum albumin	597:616	bovine serum albumin	597:616	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	5	33	theme	skin-like	937:945	arg1	layer					947:951	the compact skin-like layer	925:951	the compact skin-like layer covering the lateral wall which stayed in contact with the mold during gelification	925:1035	The cup-like, scarcely interconnected porous network, with a gradient of hardness along the cylinder axis, and the compact skin-like layer covering the lateral wall which stayed in contact with the mold during gelification, explain the preferred erosion direction and the long-term protein release.
32175650	6	34	from	effect	1126:1131	arg1	structure					1168:1176	hydrogel structure	1159:1176	hydrogel structure	1159:1176	The possible effect of the molding process on hydrogel structure suggests that molding could be a simple and cheap way to favor surface compaction and directional scaffold degradation.
32175650	1	35	theme	controlled	217:226	arg1	formulations					228:239	novel, robust, and controlled formulations	198:239	novel, robust, and controlled formulations	198:239	The oral delivery of macromolecular therapeutics to the intestinal tract requires novel, robust, and controlled formulations.
32175650	0	36	theme	oriented	55:62	arg1	degradation					64:74	spatially oriented degradation	45:74	spatially oriented degradation	45:74	Composite nanocellulose-based hydrogels with spatially oriented degradation and retarded release of macromolecules.
32175650	3	37	theme	scaffold	502:509	arg1	degradation					511:521	scaffold degradation	502:521	scaffold degradation	502:521	CNCs provide excellent mechanical and chemical stress resistance, whereas Cht allows scaffold degradation by enzyme digestion.
32175650	5	38	theme	cup-like	818:825	arg1	network					859:865	The cup-like, scarcely interconnected porous network	814:865	The cup-like, scarcely interconnected porous network	814:865	The cup-like, scarcely interconnected porous network, with a gradient of hardness along the cylinder axis, and the compact skin-like layer covering the lateral wall which stayed in contact with the mold during gelification, explain the preferred erosion direction and the long-term protein release.
32175650	3	39	dep	provide	422:428	arg1	whereas					483:489	whereas	483:489	whereas	483:489	CNCs provide excellent mechanical and chemical stress resistance, whereas Cht allows scaffold degradation by enzyme digestion.
32175650	3	40	theme	chemical	455:462	arg1	resistance					471:480	excellent mechanical and chemical stress resistance	430:480	excellent mechanical and chemical stress resistance	430:480	CNCs provide excellent mechanical and chemical stress resistance, whereas Cht allows scaffold degradation by enzyme digestion.
32175650	3	41	theme	mechanical	440:449	arg1	resistance					471:480	excellent mechanical and chemical stress resistance	430:480	excellent mechanical and chemical stress resistance	430:480	CNCs provide excellent mechanical and chemical stress resistance, whereas Cht allows scaffold degradation by enzyme digestion.
32175650	5	42	theme	porous	852:857	arg1	network					859:865	The cup-like, scarcely interconnected porous network	814:865	The cup-like, scarcely interconnected porous network	814:865	The cup-like, scarcely interconnected porous network, with a gradient of hardness along the cylinder axis, and the compact skin-like layer covering the lateral wall which stayed in contact with the mold during gelification, explain the preferred erosion direction and the long-term protein release.
32175650	3	43	theme	stress	464:469	arg1	resistance					471:480	excellent mechanical and chemical stress resistance	430:480	excellent mechanical and chemical stress resistance	430:480	CNCs provide excellent mechanical and chemical stress resistance, whereas Cht allows scaffold degradation by enzyme digestion.
32175650	6	44	theme	scaffold	1276:1283	arg1	degradation					1285:1295	directional scaffold degradation	1264:1295	directional scaffold degradation	1264:1295	The possible effect of the molding process on hydrogel structure suggests that molding could be a simple and cheap way to favor surface compaction and directional scaffold degradation.
32175650	4	45	theme	hydrogel	699:706	arg1	meanwhile					684:692	meanwhile	684:692	meanwhile	684:692	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	4	45	theme	hydrogel	699:706	arg1	scaffold					708:715	the hydrogel scaffold	695:715	the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis	695:811	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	6	46	theme	molding	1140:1146	arg1	process					1148:1154	the molding process	1136:1154	the molding process	1136:1154	The possible effect of the molding process on hydrogel structure suggests that molding could be a simple and cheap way to favor surface compaction and directional scaffold degradation.
32175650	5	47	with	network	859:865	arg1	gradient					875:882	a gradient	873:882	a gradient of hardness along the cylinder axis	873:918	The cup-like, scarcely interconnected porous network, with a gradient of hardness along the cylinder axis, and the compact skin-like layer covering the lateral wall which stayed in contact with the mold during gelification, explain the preferred erosion direction and the long-term protein release.
32175650	4	48	theme	vertical	799:806	arg1	axis					808:811	the vertical axis	795:811	the vertical axis	795:811	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	1	49	theme	macromolecular	137:150	arg1	therapeutics					152:163	macromolecular therapeutics	137:163	macromolecular therapeutics	137:163	The oral delivery of macromolecular therapeutics to the intestinal tract requires novel, robust, and controlled formulations.
32175650	5	50	dep	cup-like	818:825	arg1	interconnected					837:850	interconnected	837:850	interconnected	837:850	The cup-like, scarcely interconnected porous network, with a gradient of hardness along the cylinder axis, and the compact skin-like layer covering the lateral wall which stayed in contact with the mold during gelification, explain the preferred erosion direction and the long-term protein release.
32175650	1	51	theme	therapeutics	152:163	arg1	delivery					125:132	The oral delivery	116:132	The oral delivery of macromolecular therapeutics to the intestinal tract	116:187	The oral delivery of macromolecular therapeutics to the intestinal tract requires novel, robust, and controlled formulations.
32175650	4	52	theme	preferred	741:749	arg1	cylinder					770:777	the cylinder	766:777	the cylinder is eroded along the vertical axis	766:811	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	4	52	theme	preferred	741:749	arg1	route					751:755	a preferred route	739:755	a preferred route	739:755	The release of a representative medium size protein (bovine serum albumin) dispersed in the hydrogel is slow and shows a sigmoidal profile; meanwhile, the hydrogel scaffold degrades according to a preferred route, that is the cylinder is eroded along the vertical axis.
32175650	6	53	theme	cheap	1222:1226	arg1	molding					1192:1198	molding	1192:1198	molding	1192:1198	The possible effect of the molding process on hydrogel structure suggests that molding could be a simple and cheap way to favor surface compaction and directional scaffold degradation.
32175650	6	53	theme	cheap	1222:1226	arg1	way					1228:1230	a simple and cheap way	1209:1230	a simple and cheap way to favor surface compaction and directional scaffold degradation	1209:1295	The possible effect of the molding process on hydrogel structure suggests that molding could be a simple and cheap way to favor surface compaction and directional scaffold degradation.
32175650	6	54	theme	process	1148:1154	arg1	effect					1126:1131	The possible effect	1113:1131	The possible effect of the molding process on hydrogel structure	1113:1176	The possible effect of the molding process on hydrogel structure suggests that molding could be a simple and cheap way to favor surface compaction and directional scaffold degradation.
32175650	2	55	theme	delivery	398:405	arg1	vehicles					407:414	delivery vehicles	398:414	delivery vehicles	398:414	Here, we report on fabrication by molding of composite hydrogel cylinders made of cellulose nanocrystals (CNCs) and chitosan (Cht) and their performance as delivery vehicles.
32175650	5	56	theme	lateral	966:972	arg1	wall					974:977	the lateral wall	962:977	the lateral wall which stayed in contact with the mold during gelification	962:1035	The cup-like, scarcely interconnected porous network, with a gradient of hardness along the cylinder axis, and the compact skin-like layer covering the lateral wall which stayed in contact with the mold during gelification, explain the preferred erosion direction and the long-term protein release.
32175650	3	57	theme	enzyme	526:531	arg1	digestion					533:541	enzyme digestion	526:541	enzyme digestion	526:541	CNCs provide excellent mechanical and chemical stress resistance, whereas Cht allows scaffold degradation by enzyme digestion.
32175650	6	58	theme	possible	1117:1124	arg1	effect					1126:1131	The possible effect	1113:1131	The possible effect of the molding process on hydrogel structure	1113:1176	The possible effect of the molding process on hydrogel structure suggests that molding could be a simple and cheap way to favor surface compaction and directional scaffold degradation.
32175650	0	59	theme	retarded	80:87	arg1	release					89:95	retarded release	80:95	retarded release of macromolecules	80:113	Composite nanocellulose-based hydrogels with spatially oriented degradation and retarded release of macromolecules.
32175650	1	60	theme	intestinal	172:181	arg1	tract					183:187	the intestinal tract	168:187	the intestinal tract	168:187	The oral delivery of macromolecular therapeutics to the intestinal tract requires novel, robust, and controlled formulations.
33142622	0	0	theme	titania	112:118	arg1	surfaces					129:136	titania nanotube surfaces	112:136	titania nanotube surfaces	112:136	Tanfloc/heparin polyelectrolyte multilayers improve osteogenic differentiation of adipose-derived stem cells on titania nanotube surfaces.
33142622	3	1	used	used	688:691	arg2	SEM					606:608	SEM	606:608	SEM	606:608	Scanning electron microscopy (SEM), water contact angle measurements, and X-ray photoelectron spectroscopy were used to characterize the surfaces.
33142622	3	1	used	used	688:691	arg2	microscopy					594:603	Scanning electron microscopy	576:603	Scanning electron microscopy (SEM)	576:609	Scanning electron microscopy (SEM), water contact angle measurements, and X-ray photoelectron spectroscopy were used to characterize the surfaces.
33142622	3	1	used	used	688:691	arg2	spectroscopy					670:681	X-ray photoelectron spectroscopy	650:681	X-ray photoelectron spectroscopy	650:681	Scanning electron microscopy (SEM), water contact angle measurements, and X-ray photoelectron spectroscopy were used to characterize the surfaces.
33142622	3	1	used	used	688:691	arg2	measurements					632:643	water contact angle measurements	612:643	water contact angle measurements	612:643	Scanning electron microscopy (SEM), water contact angle measurements, and X-ray photoelectron spectroscopy were used to characterize the surfaces.
33142622	6	2	theme	ADSCs	1143:1147	arg1	differentiation					1124:1138	osteogenic differentiation	1113:1138	osteogenic differentiation of ADSCs as well as the bone mineral deposition	1113:1186	The Tanfloc/heparin PEMs on titania nanotubes improved the rate of osteogenic differentiation of ADSCs as well as the bone mineral deposition, and is therefore a promising approach for use in orthopedic implants.
33142622	1	3	theme	rapid	272:276	arg1	osseointegration					293:308	rapid and successful osseointegration	272:308	rapid and successful osseointegration of orthopedic implants	272:331	In this study, a surface modification strategy using natural biopolymers on titanium is proposed to improve bone healing and promote rapid and successful osseointegration of orthopedic implants.
33142622	2	4	theme	polyelectrolyte	439:453	arg1	PEMs					468:471	PEMs	468:471	PEMs	468:471	Titania nanotubes were fabricated via an anodization process and the surfaces were further modified with polyelectrolyte multilayers (PEMs) based on Tanfloc (a cationic tannin derivative) and glycosaminoglycans (heparin and hyaluronic acid).
33142622	2	4	theme	polyelectrolyte	439:453	arg1	multilayers					455:465	polyelectrolyte multilayers	439:465	polyelectrolyte multilayers (PEMs) based on Tanfloc (a cationic tannin derivative) and glycosaminoglycans (heparin and hyaluronic acid)	439:573	Titania nanotubes were fabricated via an anodization process and the surfaces were further modified with polyelectrolyte multilayers (PEMs) based on Tanfloc (a cationic tannin derivative) and glycosaminoglycans (heparin and hyaluronic acid).
33142622	4	5	theme	stem	739:742	arg1	ADSCs					751:755	ADSCs	751:755	ADSCs	751:755	Adipose-derived stem cells (ADSCs) were seeded on the surfaces, and the cell viability, adhesion, and proliferation were investigated.
33142622	4	5	theme	stem	739:742	arg1	cells					744:748	Adipose-derived stem cells	723:748	Adipose-derived stem cells (ADSCs)	723:756	Adipose-derived stem cells (ADSCs) were seeded on the surfaces, and the cell viability, adhesion, and proliferation were investigated.
33142622	3	6	theme	Scanning	576:583	arg1	SEM					606:608	SEM	606:608	SEM	606:608	Scanning electron microscopy (SEM), water contact angle measurements, and X-ray photoelectron spectroscopy were used to characterize the surfaces.
33142622	3	6	theme	Scanning	576:583	arg1	microscopy					594:603	Scanning electron microscopy	576:603	Scanning electron microscopy (SEM)	576:609	Scanning electron microscopy (SEM), water contact angle measurements, and X-ray photoelectron spectroscopy were used to characterize the surfaces.
33142622	5	7	theme	expression	990:999	arg1	assays					1001:1006	mineralization and protein expression assays	963:1006	mineralization and protein expression assays	963:1006	Osteogenesis was induced and osteogenic differentiation of human ADSCs on the surfaces was evaluated via mineralization and protein expression assays, immunofluorescent staining, and SEM.
33142622	1	8	theme	successful	282:291	arg1	osseointegration					293:308	rapid and successful osseointegration	272:308	rapid and successful osseointegration of orthopedic implants	272:331	In this study, a surface modification strategy using natural biopolymers on titanium is proposed to improve bone healing and promote rapid and successful osseointegration of orthopedic implants.
33142622	0	9	theme	nanotube	120:127	arg1	surfaces					129:136	titania nanotube surfaces	112:136	titania nanotube surfaces	112:136	Tanfloc/heparin polyelectrolyte multilayers improve osteogenic differentiation of adipose-derived stem cells on titania nanotube surfaces.
33142622	6	10	theme	promising	1208:1216	arg1	approach					1218:1225	a promising approach	1206:1225	a promising approach for use in orthopedic implants	1206:1256	The Tanfloc/heparin PEMs on titania nanotubes improved the rate of osteogenic differentiation of ADSCs as well as the bone mineral deposition, and is therefore a promising approach for use in orthopedic implants.
33142622	3	11	theme	electron	585:592	arg1	SEM					606:608	SEM	606:608	SEM	606:608	Scanning electron microscopy (SEM), water contact angle measurements, and X-ray photoelectron spectroscopy were used to characterize the surfaces.
33142622	3	11	theme	electron	585:592	arg1	microscopy					594:603	Scanning electron microscopy	576:603	Scanning electron microscopy (SEM)	576:609	Scanning electron microscopy (SEM), water contact angle measurements, and X-ray photoelectron spectroscopy were used to characterize the surfaces.
33142622	6	12	theme	differentiation	1124:1138	arg1	rate					1105:1108	the rate	1101:1108	the rate of osteogenic differentiation of ADSCs as well as the bone mineral deposition	1101:1186	The Tanfloc/heparin PEMs on titania nanotubes improved the rate of osteogenic differentiation of ADSCs as well as the bone mineral deposition, and is therefore a promising approach for use in orthopedic implants.
33142622	4	13	theme	cell	795:798	arg1	viability					800:808	the cell viability	791:808	the cell viability	791:808	Adipose-derived stem cells (ADSCs) were seeded on the surfaces, and the cell viability, adhesion, and proliferation were investigated.
33142622	2	14	mod	modified	425:432	arg3	multilayers					455:465	polyelectrolyte multilayers	439:465	polyelectrolyte multilayers (PEMs) based on Tanfloc (a cationic tannin derivative) and glycosaminoglycans (heparin and hyaluronic acid)	439:573	Titania nanotubes were fabricated via an anodization process and the surfaces were further modified with polyelectrolyte multilayers (PEMs) based on Tanfloc (a cationic tannin derivative) and glycosaminoglycans (heparin and hyaluronic acid).
33142622	2	14	mod	modified	425:432	arg1	surfaces					403:410	the surfaces	399:410	the surfaces	399:410	Titania nanotubes were fabricated via an anodization process and the surfaces were further modified with polyelectrolyte multilayers (PEMs) based on Tanfloc (a cationic tannin derivative) and glycosaminoglycans (heparin and hyaluronic acid).
33142622	2	14	mod	modified	425:432	arg3	PEMs					468:471	PEMs	468:471	PEMs	468:471	Titania nanotubes were fabricated via an anodization process and the surfaces were further modified with polyelectrolyte multilayers (PEMs) based on Tanfloc (a cationic tannin derivative) and glycosaminoglycans (heparin and hyaluronic acid).
33142622	1	15	theme	natural	192:198	arg1	biopolymers					200:210	natural biopolymers	192:210	natural biopolymers	192:210	In this study, a surface modification strategy using natural biopolymers on titanium is proposed to improve bone healing and promote rapid and successful osseointegration of orthopedic implants.
33142622	0	16	link	adipose-derived	82:96	arg1	cells					103:107	adipose-derived stem cells	82:107	adipose-derived stem cells	82:107	Tanfloc/heparin polyelectrolyte multilayers improve osteogenic differentiation of adipose-derived stem cells on titania nanotube surfaces.
33142622	5	17	theme	osteogenic	887:896	arg1	differentiation					898:912	osteogenic differentiation	887:912	osteogenic differentiation of human ADSCs on the surfaces	887:943	Osteogenesis was induced and osteogenic differentiation of human ADSCs on the surfaces was evaluated via mineralization and protein expression assays, immunofluorescent staining, and SEM.
33142622	0	18	theme	polyelectrolyte	16:30	arg1	multilayers					32:42	Tanfloc/heparin polyelectrolyte multilayers	0:42	Tanfloc/heparin polyelectrolyte multilayers	0:42	Tanfloc/heparin polyelectrolyte multilayers improve osteogenic differentiation of adipose-derived stem cells on titania nanotube surfaces.
33142622	5	19	theme	mineralization	963:976	arg1	assays					1001:1006	mineralization and protein expression assays	963:1006	mineralization and protein expression assays	963:1006	Osteogenesis was induced and osteogenic differentiation of human ADSCs on the surfaces was evaluated via mineralization and protein expression assays, immunofluorescent staining, and SEM.
33142622	0	20	theme	Tanfloc/heparin	0:14	arg1	multilayers					32:42	Tanfloc/heparin polyelectrolyte multilayers	0:42	Tanfloc/heparin polyelectrolyte multilayers	0:42	Tanfloc/heparin polyelectrolyte multilayers improve osteogenic differentiation of adipose-derived stem cells on titania nanotube surfaces.
33142622	2	21	dep	Tanfloc	483:489	arg1	derivative					510:519	a cationic tannin derivative	492:519	Tanfloc (a cationic tannin derivative)	483:520	Titania nanotubes were fabricated via an anodization process and the surfaces were further modified with polyelectrolyte multilayers (PEMs) based on Tanfloc (a cationic tannin derivative) and glycosaminoglycans (heparin and hyaluronic acid).
33142622	1	22	theme	orthopedic	313:322	arg1	implants					324:331	orthopedic implants	313:331	orthopedic implants	313:331	In this study, a surface modification strategy using natural biopolymers on titanium is proposed to improve bone healing and promote rapid and successful osseointegration of orthopedic implants.
33142622	4	23	link	Adipose-derived	723:737	arg1	ADSCs					751:755	ADSCs	751:755	ADSCs	751:755	Adipose-derived stem cells (ADSCs) were seeded on the surfaces, and the cell viability, adhesion, and proliferation were investigated.
33142622	4	23	link	Adipose-derived	723:737	arg1	cells					744:748	Adipose-derived stem cells	723:748	Adipose-derived stem cells (ADSCs)	723:756	Adipose-derived stem cells (ADSCs) were seeded on the surfaces, and the cell viability, adhesion, and proliferation were investigated.
33142622	1	24	theme	implants	324:331	arg1	osseointegration					293:308	rapid and successful osseointegration	272:308	rapid and successful osseointegration of orthopedic implants	272:331	In this study, a surface modification strategy using natural biopolymers on titanium is proposed to improve bone healing and promote rapid and successful osseointegration of orthopedic implants.
33142622	5	25	theme	immunofluorescent	1009:1025	arg1	staining					1027:1034	immunofluorescent staining	1009:1034	immunofluorescent staining	1009:1034	Osteogenesis was induced and osteogenic differentiation of human ADSCs on the surfaces was evaluated via mineralization and protein expression assays, immunofluorescent staining, and SEM.
33142622	2	26	theme	Titania	334:340	arg1	nanotubes					342:350	Titania nanotubes	334:350	Titania nanotubes	334:350	Titania nanotubes were fabricated via an anodization process and the surfaces were further modified with polyelectrolyte multilayers (PEMs) based on Tanfloc (a cationic tannin derivative) and glycosaminoglycans (heparin and hyaluronic acid).
33142622	2	27	theme	cationic	494:501	arg1	derivative					510:519	a cationic tannin derivative	492:519	Tanfloc (a cationic tannin derivative)	483:520	Titania nanotubes were fabricated via an anodization process and the surfaces were further modified with polyelectrolyte multilayers (PEMs) based on Tanfloc (a cationic tannin derivative) and glycosaminoglycans (heparin and hyaluronic acid).
33142622	4	28	theme	Adipose-derived	723:737	arg1	ADSCs					751:755	ADSCs	751:755	ADSCs	751:755	Adipose-derived stem cells (ADSCs) were seeded on the surfaces, and the cell viability, adhesion, and proliferation were investigated.
33142622	4	28	theme	Adipose-derived	723:737	arg1	cells					744:748	Adipose-derived stem cells	723:748	Adipose-derived stem cells (ADSCs)	723:756	Adipose-derived stem cells (ADSCs) were seeded on the surfaces, and the cell viability, adhesion, and proliferation were investigated.
33142622	6	29	theme	bone	1164:1167	arg1	deposition					1177:1186	the bone mineral deposition	1160:1186	ADSCs as well as the bone mineral deposition	1143:1186	The Tanfloc/heparin PEMs on titania nanotubes improved the rate of osteogenic differentiation of ADSCs as well as the bone mineral deposition, and is therefore a promising approach for use in orthopedic implants.
33142622	3	30	theme	contact	618:624	arg1	measurements					632:643	water contact angle measurements	612:643	water contact angle measurements	612:643	Scanning electron microscopy (SEM), water contact angle measurements, and X-ray photoelectron spectroscopy were used to characterize the surfaces.
33142622	2	31	theme	tannin	503:508	arg1	derivative					510:519	a cationic tannin derivative	492:519	Tanfloc (a cationic tannin derivative)	483:520	Titania nanotubes were fabricated via an anodization process and the surfaces were further modified with polyelectrolyte multilayers (PEMs) based on Tanfloc (a cationic tannin derivative) and glycosaminoglycans (heparin and hyaluronic acid).
33142622	0	32	theme	osteogenic	52:61	arg1	differentiation					63:77	osteogenic differentiation	52:77	osteogenic differentiation of adipose-derived stem cells	52:107	Tanfloc/heparin polyelectrolyte multilayers improve osteogenic differentiation of adipose-derived stem cells on titania nanotube surfaces.
33142622	3	33	theme	photoelectron	656:668	arg1	spectroscopy					670:681	X-ray photoelectron spectroscopy	650:681	X-ray photoelectron spectroscopy	650:681	Scanning electron microscopy (SEM), water contact angle measurements, and X-ray photoelectron spectroscopy were used to characterize the surfaces.
33142622	3	34	theme	angle	626:630	arg1	measurements					632:643	water contact angle measurements	612:643	water contact angle measurements	612:643	Scanning electron microscopy (SEM), water contact angle measurements, and X-ray photoelectron spectroscopy were used to characterize the surfaces.
33142622	6	35	theme	titania	1074:1080	arg1	nanotubes					1082:1090	titania nanotubes	1074:1090	titania nanotubes	1074:1090	The Tanfloc/heparin PEMs on titania nanotubes improved the rate of osteogenic differentiation of ADSCs as well as the bone mineral deposition, and is therefore a promising approach for use in orthopedic implants.
33142622	0	36	theme	adipose-derived	82:96	arg1	cells					103:107	adipose-derived stem cells	82:107	adipose-derived stem cells	82:107	Tanfloc/heparin polyelectrolyte multilayers improve osteogenic differentiation of adipose-derived stem cells on titania nanotube surfaces.
33142622	5	37	theme	human	917:921	arg1	ADSCs					923:927	human ADSCs	917:927	human ADSCs	917:927	Osteogenesis was induced and osteogenic differentiation of human ADSCs on the surfaces was evaluated via mineralization and protein expression assays, immunofluorescent staining, and SEM.
33142622	6	38	theme	mineral	1169:1175	arg1	deposition					1177:1186	the bone mineral deposition	1160:1186	ADSCs as well as the bone mineral deposition	1143:1186	The Tanfloc/heparin PEMs on titania nanotubes improved the rate of osteogenic differentiation of ADSCs as well as the bone mineral deposition, and is therefore a promising approach for use in orthopedic implants.
33142622	3	39	theme	water	612:616	arg1	measurements					632:643	water contact angle measurements	612:643	water contact angle measurements	612:643	Scanning electron microscopy (SEM), water contact angle measurements, and X-ray photoelectron spectroscopy were used to characterize the surfaces.
33142622	2	40	theme	anodization	375:385	arg1	process					387:393	an anodization process	372:393	an anodization process	372:393	Titania nanotubes were fabricated via an anodization process and the surfaces were further modified with polyelectrolyte multilayers (PEMs) based on Tanfloc (a cationic tannin derivative) and glycosaminoglycans (heparin and hyaluronic acid).
33142622	6	41	theme	orthopedic	1238:1247	arg1	implants					1249:1256	orthopedic implants	1238:1256	orthopedic implants	1238:1256	The Tanfloc/heparin PEMs on titania nanotubes improved the rate of osteogenic differentiation of ADSCs as well as the bone mineral deposition, and is therefore a promising approach for use in orthopedic implants.
33142622	5	42	theme	protein	982:988	arg1	assays					1001:1006	mineralization and protein expression assays	963:1006	mineralization and protein expression assays	963:1006	Osteogenesis was induced and osteogenic differentiation of human ADSCs on the surfaces was evaluated via mineralization and protein expression assays, immunofluorescent staining, and SEM.
33142622	1	43	theme	bone	247:250	arg1	healing					252:258	bone healing	247:258	bone healing	247:258	In this study, a surface modification strategy using natural biopolymers on titanium is proposed to improve bone healing and promote rapid and successful osseointegration of orthopedic implants.
33142622	2	44	dep	glycosaminoglycans	526:543	arg1	glycosaminoglycans					526:543	glycosaminoglycans	526:543	glycosaminoglycans (heparin and hyaluronic acid)	526:573	Titania nanotubes were fabricated via an anodization process and the surfaces were further modified with polyelectrolyte multilayers (PEMs) based on Tanfloc (a cationic tannin derivative) and glycosaminoglycans (heparin and hyaluronic acid).
33142622	2	44	dep	glycosaminoglycans	526:543	arg1	heparin					546:552	heparin	546:552	heparin	546:552	Titania nanotubes were fabricated via an anodization process and the surfaces were further modified with polyelectrolyte multilayers (PEMs) based on Tanfloc (a cationic tannin derivative) and glycosaminoglycans (heparin and hyaluronic acid).
33142622	2	44	dep	glycosaminoglycans	526:543	arg1	acid					569:572	hyaluronic acid	558:572	hyaluronic acid	558:572	Titania nanotubes were fabricated via an anodization process and the surfaces were further modified with polyelectrolyte multilayers (PEMs) based on Tanfloc (a cationic tannin derivative) and glycosaminoglycans (heparin and hyaluronic acid).
33142622	3	45	theme	X-ray	650:654	arg1	spectroscopy					670:681	X-ray photoelectron spectroscopy	650:681	X-ray photoelectron spectroscopy	650:681	Scanning electron microscopy (SEM), water contact angle measurements, and X-ray photoelectron spectroscopy were used to characterize the surfaces.
33142622	5	46	from	differentiation	898:912	arg1	surfaces					936:943	the surfaces	932:943	the surfaces	932:943	Osteogenesis was induced and osteogenic differentiation of human ADSCs on the surfaces was evaluated via mineralization and protein expression assays, immunofluorescent staining, and SEM.
33142622	5	47	theme	ADSCs	923:927	arg1	differentiation					898:912	osteogenic differentiation	887:912	osteogenic differentiation of human ADSCs on the surfaces	887:943	Osteogenesis was induced and osteogenic differentiation of human ADSCs on the surfaces was evaluated via mineralization and protein expression assays, immunofluorescent staining, and SEM.
33142622	0	48	theme	cells	103:107	arg1	differentiation					63:77	osteogenic differentiation	52:77	osteogenic differentiation of adipose-derived stem cells	52:107	Tanfloc/heparin polyelectrolyte multilayers improve osteogenic differentiation of adipose-derived stem cells on titania nanotube surfaces.
33142622	6	49	from	PEMs	1066:1069	arg1	nanotubes					1082:1090	titania nanotubes	1074:1090	titania nanotubes	1074:1090	The Tanfloc/heparin PEMs on titania nanotubes improved the rate of osteogenic differentiation of ADSCs as well as the bone mineral deposition, and is therefore a promising approach for use in orthopedic implants.
33142622	1	50	theme	surface	156:162	arg1	strategy					177:184	a surface modification strategy	154:184	a surface modification strategy using natural biopolymers on titanium	154:222	In this study, a surface modification strategy using natural biopolymers on titanium is proposed to improve bone healing and promote rapid and successful osseointegration of orthopedic implants.
33142622	6	51	theme	osteogenic	1113:1122	arg1	differentiation					1124:1138	osteogenic differentiation	1113:1138	osteogenic differentiation of ADSCs as well as the bone mineral deposition	1113:1186	The Tanfloc/heparin PEMs on titania nanotubes improved the rate of osteogenic differentiation of ADSCs as well as the bone mineral deposition, and is therefore a promising approach for use in orthopedic implants.
33142622	6	52	theme	Tanfloc/heparin	1050:1064	arg1	PEMs					1066:1069	The Tanfloc/heparin PEMs	1046:1069	The Tanfloc/heparin PEMs on titania nanotubes	1046:1090	The Tanfloc/heparin PEMs on titania nanotubes improved the rate of osteogenic differentiation of ADSCs as well as the bone mineral deposition, and is therefore a promising approach for use in orthopedic implants.
33142622	0	53	theme	stem	98:101	arg1	cells					103:107	adipose-derived stem cells	82:107	adipose-derived stem cells	82:107	Tanfloc/heparin polyelectrolyte multilayers improve osteogenic differentiation of adipose-derived stem cells on titania nanotube surfaces.
33142622	6	54	theme	deposition	1177:1186	arg1	differentiation					1124:1138	osteogenic differentiation	1113:1138	osteogenic differentiation of ADSCs as well as the bone mineral deposition	1113:1186	The Tanfloc/heparin PEMs on titania nanotubes improved the rate of osteogenic differentiation of ADSCs as well as the bone mineral deposition, and is therefore a promising approach for use in orthopedic implants.
33142622	1	55	theme	modification	164:175	arg1	strategy					177:184	a surface modification strategy	154:184	a surface modification strategy using natural biopolymers on titanium	154:222	In this study, a surface modification strategy using natural biopolymers on titanium is proposed to improve bone healing and promote rapid and successful osseointegration of orthopedic implants.
33142622	6	56	from	use	1231:1233	arg1	implants					1249:1256	orthopedic implants	1238:1256	orthopedic implants	1238:1256	The Tanfloc/heparin PEMs on titania nanotubes improved the rate of osteogenic differentiation of ADSCs as well as the bone mineral deposition, and is therefore a promising approach for use in orthopedic implants.
33142622	2	57	theme	hyaluronic	558:567	arg1	glycosaminoglycans					526:543	glycosaminoglycans	526:543	glycosaminoglycans (heparin and hyaluronic acid)	526:573	Titania nanotubes were fabricated via an anodization process and the surfaces were further modified with polyelectrolyte multilayers (PEMs) based on Tanfloc (a cationic tannin derivative) and glycosaminoglycans (heparin and hyaluronic acid).
33142622	2	57	theme	hyaluronic	558:567	arg1	acid					569:572	hyaluronic acid	558:572	hyaluronic acid	558:572	Titania nanotubes were fabricated via an anodization process and the surfaces were further modified with polyelectrolyte multilayers (PEMs) based on Tanfloc (a cationic tannin derivative) and glycosaminoglycans (heparin and hyaluronic acid).
32856644	7	0	from	content	1350:1356	arg1	range					1381:1385	the range	1377:1385	the range of 0-20 wt%	1377:1397	Moreover, these promoting effects of CHWs are positively related to the content of the whiskers in the range of 0-20 wt%.
32856644	2	1	theme	PLLA	528:531	arg1	matrix					533:538	the PLLA matrix	524:538	the PLLA matrix	524:538	Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix.
32856644	3	2	theme	dibutyl	633:639	arg1	DBP					652:654	DBP	652:654	DBP	652:654	Moreover, a trisolvent system, including dichloromethane (DCM), 2-butoxyethanlol (2-Bu) and dibutyl phthalate (DBP), instead of a single solvent system of DCM was chosen to prepare CHW/PLLA (CP) composite inks.
32856644	3	2	theme	dibutyl	633:639	arg1	phthalate					641:649	dibutyl phthalate	633:649	dibutyl phthalate (DBP)	633:655	Moreover, a trisolvent system, including dichloromethane (DCM), 2-butoxyethanlol (2-Bu) and dibutyl phthalate (DBP), instead of a single solvent system of DCM was chosen to prepare CHW/PLLA (CP) composite inks.
32856644	4	3	theme	CP	762:763	arg1	scaffolds					782:790	the CP porous composite scaffolds	758:790	the CP porous composite scaffolds	758:790	Then, the CP porous composite scaffolds were further fabricated via the direct ink writing method.
32856644	10	4	theme	composite	1877:1885	arg1	scaffolds					1887:1895	These robust CP composite scaffolds	1861:1895	These robust CP composite scaffolds	1861:1895	These robust CP composite scaffolds offer a new route for bone tissue engineering application.
32856644	1	5	theme	3D	246:247	arg1	printing					249:256	3D printing	246:256	3D printing	246:256	Although poly(l-lactide) (PLLA) based porous scaffolds have been widely fabricated through 3D printing, their poor mechanical properties and osteogenic activity still do not meet the needs of bone tissue repair.
32856644	5	6	contain	have	889:892	arg2	structures					909:918	good 3D porous structures	894:918	good 3D porous structures	894:918	The as-printed CP composite scaffolds have good 3D porous structures with a pore size of 400 ± 14 μm and a porosity of 80 ± 5%.
32856644	5	6	contain	have	889:892	arg1	scaffolds					879:887	The as-printed CP composite scaffolds	851:887	The as-printed CP composite scaffolds	851:887	The as-printed CP composite scaffolds have good 3D porous structures with a pore size of 400 ± 14 μm and a porosity of 80 ± 5%.
32856644	6	7	theme	PLLA	1002:1005	arg1	scaffold					1007:1014	the pure PLLA scaffold	993:1014	the pure PLLA scaffold	993:1014	Compared with the pure PLLA scaffold, the CP composite scaffolds showed significantly superior hydrophilicity and compression performance, and also were more conducive to cell adhesion, proliferation, and up-regulating alkaline phosphate activity and calcium deposition due to the presence of CHWs.
32856644	9	8	contain	containing	1734:1743	arg2	content					1758:1764	a lower mass content	1745:1764	a lower mass content of CHWs	1745:1772	Interestingly, compared with these scaffolds containing a lower mass content of CHWs, only the 40%CP composite scaffold exhibited significant anti-inflammatory properties.
32856644	9	8	contain	containing	1734:1743	arg1	scaffolds					1724:1732	these scaffolds	1718:1732	these scaffolds containing a lower mass content of CHWs	1718:1772	Interestingly, compared with these scaffolds containing a lower mass content of CHWs, only the 40%CP composite scaffold exhibited significant anti-inflammatory properties.
32856644	8	9	theme	composite	1668:1676	arg1	scaffolds					1678:1686	other composite scaffolds	1662:1686	other composite scaffolds	1662:1686	However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds.
32856644	2	10	contain	having	399:404	arg2	properties					429:438	outstanding mechanical properties	406:438	outstanding mechanical properties	406:438	Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix.
32856644	2	10	contain	having	399:404	arg1	CHWs					392:395	CHWs	392:395	CHWs	392:395	Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix.
32856644	2	10	contain	having	399:404	arg1	whiskers					382:389	chitin whiskers	375:389	chitin whiskers (CHWs)	375:396	Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix.
32856644	8	11	theme	osteogenic	1508:1517	arg1	activity					1519:1526	osteogenic activity	1508:1526	osteogenic activity	1508:1526	However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds.
32856644	7	12	theme	promoting	1294:1302	arg1	effects					1304:1310	these promoting effects	1288:1310	these promoting effects of CHWs	1288:1318	Moreover, these promoting effects of CHWs are positively related to the content of the whiskers in the range of 0-20 wt%.
32856644	0	13	theme	tissue	135:140	arg1	engineering					142:152	bone tissue engineering	130:152	bone tissue engineering	130:152	Fabrication and evaluation of a chitin whisker/poly(L-lactide) composite scaffold by the direct trisolvent-ink writing method for bone tissue engineering.
32856644	0	14	theme	writing	111:117	arg1	method					119:124	the direct trisolvent-ink writing method	85:124	the direct trisolvent-ink writing method for bone tissue engineering	85:152	Fabrication and evaluation of a chitin whisker/poly(L-lactide) composite scaffold by the direct trisolvent-ink writing method for bone tissue engineering.
32856644	1	15	theme	bone	347:350	arg1	repair					359:364	bone tissue repair	347:364	bone tissue repair	347:364	Although poly(l-lactide) (PLLA) based porous scaffolds have been widely fabricated through 3D printing, their poor mechanical properties and osteogenic activity still do not meet the needs of bone tissue repair.
32856644	8	16	theme	corresponding	1535:1547	arg1	scaffold					1565:1572	the corresponding 40%CP composite scaffold	1531:1572	the corresponding 40%CP composite scaffold	1531:1572	However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds.
32856644	9	17	theme	lower	1747:1751	arg1	content					1758:1764	a lower mass content	1745:1764	a lower mass content of CHWs	1745:1772	Interestingly, compared with these scaffolds containing a lower mass content of CHWs, only the 40%CP composite scaffold exhibited significant anti-inflammatory properties.
32856644	8	18	theme	%	1551:1551	arg1	scaffold					1565:1572	the corresponding 40%CP composite scaffold	1531:1572	the corresponding 40%CP composite scaffold	1531:1572	However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds.
32856644	1	19	theme	based	187:191	arg1	scaffolds					200:208	poly(l-lactide) (PLLA) based porous scaffolds	164:208	poly(l-lactide) (PLLA) based porous scaffolds	164:208	Although poly(l-lactide) (PLLA) based porous scaffolds have been widely fabricated through 3D printing, their poor mechanical properties and osteogenic activity still do not meet the needs of bone tissue repair.
32856644	4	20	theme	composite	772:780	arg1	scaffolds					782:790	the CP porous composite scaffolds	758:790	the CP porous composite scaffolds	758:790	Then, the CP porous composite scaffolds were further fabricated via the direct ink writing method.
32856644	3	21	theme	single	671:676	arg1	system					686:691	a single solvent system	669:691	a trisolvent system, including dichloromethane (DCM), 2-butoxyethanlol (2-Bu) and dibutyl phthalate (DBP), instead of a single solvent system of DCM	551:698	Moreover, a trisolvent system, including dichloromethane (DCM), 2-butoxyethanlol (2-Bu) and dibutyl phthalate (DBP), instead of a single solvent system of DCM was chosen to prepare CHW/PLLA (CP) composite inks.
32856644	6	22	theme	cell	1150:1153	arg1	adhesion					1155:1162	cell adhesion	1150:1162	cell adhesion	1150:1162	Compared with the pure PLLA scaffold, the CP composite scaffolds showed significantly superior hydrophilicity and compression performance, and also were more conducive to cell adhesion, proliferation, and up-regulating alkaline phosphate activity and calcium deposition due to the presence of CHWs.
32856644	8	23	theme	composite	1555:1563	arg1	scaffold					1565:1572	the corresponding 40%CP composite scaffold	1531:1572	the corresponding 40%CP composite scaffold	1531:1572	However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds.
32856644	9	24	theme	CHWs	1769:1772	arg1	content					1758:1764	a lower mass content	1745:1764	a lower mass content of CHWs	1745:1772	Interestingly, compared with these scaffolds containing a lower mass content of CHWs, only the 40%CP composite scaffold exhibited significant anti-inflammatory properties.
32856644	6	25	theme	CP	1021:1022	arg1	conducive					1137:1145	conducive	1137:1145	conducive	1137:1145	Compared with the pure PLLA scaffold, the CP composite scaffolds showed significantly superior hydrophilicity and compression performance, and also were more conducive to cell adhesion, proliferation, and up-regulating alkaline phosphate activity and calcium deposition due to the presence of CHWs.
32856644	6	25	theme	CP	1021:1022	arg1	scaffolds					1034:1042	the CP composite scaffolds	1017:1042	the CP composite scaffolds	1017:1042	Compared with the pure PLLA scaffold, the CP composite scaffolds showed significantly superior hydrophilicity and compression performance, and also were more conducive to cell adhesion, proliferation, and up-regulating alkaline phosphate activity and calcium deposition due to the presence of CHWs.
32856644	3	26	theme	DCM	696:698	arg1	system					686:691	a single solvent system	669:691	a trisolvent system, including dichloromethane (DCM), 2-butoxyethanlol (2-Bu) and dibutyl phthalate (DBP), instead of a single solvent system of DCM	551:698	Moreover, a trisolvent system, including dichloromethane (DCM), 2-butoxyethanlol (2-Bu) and dibutyl phthalate (DBP), instead of a single solvent system of DCM was chosen to prepare CHW/PLLA (CP) composite inks.
32856644	0	27	theme	L-lactide	52:60	arg1	scaffold					73:80	a chitin whisker/poly(L-lactide) composite scaffold	30:80	a chitin whisker/poly(L-lactide) composite scaffold	30:80	Fabrication and evaluation of a chitin whisker/poly(L-lactide) composite scaffold by the direct trisolvent-ink writing method for bone tissue engineering.
32856644	5	28	theme	80	970:971	arg1	±					973:973	±	973:973	±	973:973	The as-printed CP composite scaffolds have good 3D porous structures with a pore size of 400 ± 14 μm and a porosity of 80 ± 5%.
32856644	3	29	theme	trisolvent	553:562	arg1	system					564:569	a trisolvent system	551:569	a trisolvent system	551:569	Moreover, a trisolvent system, including dichloromethane (DCM), 2-butoxyethanlol (2-Bu) and dibutyl phthalate (DBP), instead of a single solvent system of DCM was chosen to prepare CHW/PLLA (CP) composite inks.
32856644	2	30	theme	excellent	441:449	arg1	etc.					487:490	etc.	487:490	etc.	487:490	Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix.
32856644	2	30	theme	excellent	441:449	arg1	affinity					456:463	excellent cell affinity	441:463	excellent cell affinity	441:463	Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix.
32856644	2	30	theme	excellent	441:449	arg1	activity					477:484	osteogenic activity	466:484	osteogenic activity	466:484	Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix.
32856644	9	31	theme	40	1784:1785	arg1	%					1786:1786	%	1786:1786	%	1786:1786	Interestingly, compared with these scaffolds containing a lower mass content of CHWs, only the 40%CP composite scaffold exhibited significant anti-inflammatory properties.
32856644	0	32	theme	composite	63:71	arg1	scaffold					73:80	a chitin whisker/poly(L-lactide) composite scaffold	30:80	a chitin whisker/poly(L-lactide) composite scaffold	30:80	Fabrication and evaluation of a chitin whisker/poly(L-lactide) composite scaffold by the direct trisolvent-ink writing method for bone tissue engineering.
32856644	5	33	theme	5	975:975	arg1	±					973:973	±	973:973	±	973:973	The as-printed CP composite scaffolds have good 3D porous structures with a pore size of 400 ± 14 μm and a porosity of 80 ± 5%.
32856644	7	34	theme	wt	1395:1396	arg1	%					1397:1397	0-20 wt%	1390:1397	0-20 wt%	1390:1397	Moreover, these promoting effects of CHWs are positively related to the content of the whiskers in the range of 0-20 wt%.
32856644	4	35	theme	ink	831:833	arg1	method					843:848	the direct ink writing method	820:848	the direct ink writing method	820:848	Then, the CP porous composite scaffolds were further fabricated via the direct ink writing method.
32856644	9	36	theme	CP	1787:1788	arg1	scaffold					1800:1807	only the 40%CP composite scaffold	1775:1807	only the 40%CP composite scaffold	1775:1807	Interestingly, compared with these scaffolds containing a lower mass content of CHWs, only the 40%CP composite scaffold exhibited significant anti-inflammatory properties.
32856644	6	37	theme	phosphate	1207:1215	arg1	activity					1217:1224	up-regulating alkaline phosphate activity	1184:1224	up-regulating alkaline phosphate activity	1184:1224	Compared with the pure PLLA scaffold, the CP composite scaffolds showed significantly superior hydrophilicity and compression performance, and also were more conducive to cell adhesion, proliferation, and up-regulating alkaline phosphate activity and calcium deposition due to the presence of CHWs.
32856644	2	38	theme	osteogenic	466:475	arg1	affinity					456:463	excellent cell affinity	441:463	excellent cell affinity	441:463	Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix.
32856644	2	38	theme	osteogenic	466:475	arg1	activity					477:484	osteogenic activity	466:484	osteogenic activity	466:484	Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix.
32856644	0	39	theme	direct	89:94	arg1	method					119:124	the direct trisolvent-ink writing method	85:124	the direct trisolvent-ink writing method for bone tissue engineering	85:152	Fabrication and evaluation of a chitin whisker/poly(L-lactide) composite scaffold by the direct trisolvent-ink writing method for bone tissue engineering.
32856644	8	40	theme	different	1616:1624	arg1	microstructure					1626:1639	the different microstructure	1612:1639	the different microstructure of the scaffold from other composite scaffolds	1612:1686	However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds.
32856644	5	41	theme	CP	866:867	arg1	scaffolds					879:887	The as-printed CP composite scaffolds	851:887	The as-printed CP composite scaffolds	851:887	The as-printed CP composite scaffolds have good 3D porous structures with a pore size of 400 ± 14 μm and a porosity of 80 ± 5%.
32856644	9	42	theme	significant	1819:1829	arg1	properties					1849:1858	significant anti-inflammatory properties	1819:1858	significant anti-inflammatory properties	1819:1858	Interestingly, compared with these scaffolds containing a lower mass content of CHWs, only the 40%CP composite scaffold exhibited significant anti-inflammatory properties.
32856644	6	43	theme	CHWs	1272:1275	arg1	presence					1260:1267	the presence	1256:1267	the presence of CHWs	1256:1275	Compared with the pure PLLA scaffold, the CP composite scaffolds showed significantly superior hydrophilicity and compression performance, and also were more conducive to cell adhesion, proliferation, and up-regulating alkaline phosphate activity and calcium deposition due to the presence of CHWs.
32856644	1	44	theme	mechanical	270:279	arg1	properties					281:290	their poor mechanical properties	259:290	their poor mechanical properties	259:290	Although poly(l-lactide) (PLLA) based porous scaffolds have been widely fabricated through 3D printing, their poor mechanical properties and osteogenic activity still do not meet the needs of bone tissue repair.
32856644	5	45	theme	porous	902:907	arg1	structures					909:918	good 3D porous structures	894:918	good 3D porous structures	894:918	The as-printed CP composite scaffolds have good 3D porous structures with a pore size of 400 ± 14 μm and a porosity of 80 ± 5%.
32856644	2	46	theme	outstanding	406:416	arg1	properties					429:438	outstanding mechanical properties	406:438	outstanding mechanical properties	406:438	Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix.
32856644	2	47	dep	whiskers	382:389	arg1	etc.					487:490	etc.	487:490	etc.	487:490	Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix.
32856644	2	47	dep	whiskers	382:389	arg1	affinity					456:463	excellent cell affinity	441:463	excellent cell affinity	441:463	Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix.
32856644	2	47	dep	whiskers	382:389	arg1	activity					477:484	osteogenic activity	466:484	osteogenic activity	466:484	Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix.
32856644	0	48	theme	whisker/poly	39:50	arg1	scaffold					73:80	a chitin whisker/poly(L-lactide) composite scaffold	30:80	a chitin whisker/poly(L-lactide) composite scaffold	30:80	Fabrication and evaluation of a chitin whisker/poly(L-lactide) composite scaffold by the direct trisolvent-ink writing method for bone tissue engineering.
32856644	10	49	theme	CP	1874:1875	arg1	scaffolds					1887:1895	These robust CP composite scaffolds	1861:1895	These robust CP composite scaffolds	1861:1895	These robust CP composite scaffolds offer a new route for bone tissue engineering application.
32856644	1	50	dep	the	334:336	arg1	needs					338:342	needs	338:342	needs	338:342	Although poly(l-lactide) (PLLA) based porous scaffolds have been widely fabricated through 3D printing, their poor mechanical properties and osteogenic activity still do not meet the needs of bone tissue repair.
32856644	1	51	theme	porous	193:198	arg1	scaffolds					200:208	poly(l-lactide) (PLLA) based porous scaffolds	164:208	poly(l-lactide) (PLLA) based porous scaffolds	164:208	Although poly(l-lactide) (PLLA) based porous scaffolds have been widely fabricated through 3D printing, their poor mechanical properties and osteogenic activity still do not meet the needs of bone tissue repair.
32856644	5	52	theme	good	894:897	arg1	structures					909:918	good 3D porous structures	894:918	good 3D porous structures	894:918	The as-printed CP composite scaffolds have good 3D porous structures with a pore size of 400 ± 14 μm and a porosity of 80 ± 5%.
32856644	6	53	theme	calcium	1230:1236	arg1	deposition					1238:1247	calcium deposition	1230:1247	calcium deposition	1230:1247	Compared with the pure PLLA scaffold, the CP composite scaffolds showed significantly superior hydrophilicity and compression performance, and also were more conducive to cell adhesion, proliferation, and up-regulating alkaline phosphate activity and calcium deposition due to the presence of CHWs.
32856644	8	54	theme	scaffold	1648:1655	arg1	microstructure					1626:1639	the different microstructure	1612:1639	the different microstructure of the scaffold from other composite scaffolds	1612:1686	However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds.
32856644	7	55	theme	CHWs	1315:1318	arg1	effects					1304:1310	these promoting effects	1288:1310	these promoting effects of CHWs	1288:1318	Moreover, these promoting effects of CHWs are positively related to the content of the whiskers in the range of 0-20 wt%.
32856644	8	56	theme	CHWs	1427:1430	arg1	content					1416:1422	the content	1412:1422	the content of CHWs	1412:1430	However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds.
32856644	8	57	theme	other	1662:1666	arg1	scaffolds					1678:1686	other composite scaffolds	1662:1686	other composite scaffolds	1662:1686	However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds.
32856644	7	58	from	whiskers	1365:1372	arg1	range					1381:1385	the range	1377:1385	the range of 0-20 wt%	1377:1397	Moreover, these promoting effects of CHWs are positively related to the content of the whiskers in the range of 0-20 wt%.
32856644	10	59	theme	bone	1919:1922	arg1	engineering					1931:1941	bone tissue engineering	1919:1941	bone tissue engineering application	1919:1953	These robust CP composite scaffolds offer a new route for bone tissue engineering application.
32856644	10	60	theme	new	1905:1907	arg1	route					1909:1913	a new route	1903:1913	a new route for bone tissue engineering application	1903:1953	These robust CP composite scaffolds offer a new route for bone tissue engineering application.
32856644	8	61	theme	cell	1490:1493	arg1	affinity					1495:1502	cell affinity	1490:1502	cell affinity	1490:1502	However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds.
32856644	1	62	theme	poly	164:167	arg1	scaffolds					200:208	poly(l-lactide) (PLLA) based porous scaffolds	164:208	poly(l-lactide) (PLLA) based porous scaffolds	164:208	Although poly(l-lactide) (PLLA) based porous scaffolds have been widely fabricated through 3D printing, their poor mechanical properties and osteogenic activity still do not meet the needs of bone tissue repair.
32856644	6	63	theme	pure	997:1000	arg1	scaffold					1007:1014	the pure PLLA scaffold	993:1014	the pure PLLA scaffold	993:1014	Compared with the pure PLLA scaffold, the CP composite scaffolds showed significantly superior hydrophilicity and compression performance, and also were more conducive to cell adhesion, proliferation, and up-regulating alkaline phosphate activity and calcium deposition due to the presence of CHWs.
32856644	6	64	theme	compression	1093:1103	arg1	performance					1105:1115	compression performance	1093:1115	compression performance	1093:1115	Compared with the pure PLLA scaffold, the CP composite scaffolds showed significantly superior hydrophilicity and compression performance, and also were more conducive to cell adhesion, proliferation, and up-regulating alkaline phosphate activity and calcium deposition due to the presence of CHWs.
32856644	10	65	theme	engineering	1931:1941	arg1	application					1943:1953	bone tissue engineering application	1919:1953	bone tissue engineering application	1919:1953	These robust CP composite scaffolds offer a new route for bone tissue engineering application.
32856644	0	66	theme	bone	130:133	arg1	engineering					142:152	bone tissue engineering	130:152	bone tissue engineering	130:152	Fabrication and evaluation of a chitin whisker/poly(L-lactide) composite scaffold by the direct trisolvent-ink writing method for bone tissue engineering.
32856644	5	67	theme	±	944:944	arg1	μm					949:950	400 ± 14 μm	940:950	400 ± 14 μm	940:950	The as-printed CP composite scaffolds have good 3D porous structures with a pore size of 400 ± 14 μm and a porosity of 80 ± 5%.
32856644	6	68	theme	superior	1065:1072	arg1	hydrophilicity					1074:1087	significantly superior hydrophilicity	1051:1087	significantly superior hydrophilicity	1051:1087	Compared with the pure PLLA scaffold, the CP composite scaffolds showed significantly superior hydrophilicity and compression performance, and also were more conducive to cell adhesion, proliferation, and up-regulating alkaline phosphate activity and calcium deposition due to the presence of CHWs.
32856644	9	69	theme	mass	1753:1756	arg1	content					1758:1764	a lower mass content	1745:1764	a lower mass content of CHWs	1745:1772	Interestingly, compared with these scaffolds containing a lower mass content of CHWs, only the 40%CP composite scaffold exhibited significant anti-inflammatory properties.
32856644	5	70	theme	μm	949:950	arg1	size					932:935	a pore size	925:935	a pore size of 400 ± 14 μm	925:950	The as-printed CP composite scaffolds have good 3D porous structures with a pore size of 400 ± 14 μm and a porosity of 80 ± 5%.
32856644	5	70	theme	μm	949:950	arg1	porosity					958:965	a porosity	956:965	a porosity of 80 ± 5%	956:976	The as-printed CP composite scaffolds have good 3D porous structures with a pore size of 400 ± 14 μm and a porosity of 80 ± 5%.
32856644	8	71	theme	40	1549:1550	arg1	%					1551:1551	%	1551:1551	%	1551:1551	However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds.
32856644	4	72	theme	porous	765:770	arg1	scaffolds					782:790	the CP porous composite scaffolds	758:790	the CP porous composite scaffolds	758:790	Then, the CP porous composite scaffolds were further fabricated via the direct ink writing method.
32856644	3	73	theme	solvent	678:684	arg1	system					686:691	a single solvent system	669:691	a trisolvent system, including dichloromethane (DCM), 2-butoxyethanlol (2-Bu) and dibutyl phthalate (DBP), instead of a single solvent system of DCM	551:698	Moreover, a trisolvent system, including dichloromethane (DCM), 2-butoxyethanlol (2-Bu) and dibutyl phthalate (DBP), instead of a single solvent system of DCM was chosen to prepare CHW/PLLA (CP) composite inks.
32856644	5	74	theme	pore	927:930	arg1	size					932:935	a pore size	925:935	a pore size of 400 ± 14 μm	925:950	The as-printed CP composite scaffolds have good 3D porous structures with a pore size of 400 ± 14 μm and a porosity of 80 ± 5%.
32856644	8	75	theme	CP	1552:1553	arg1	scaffold					1565:1572	the corresponding 40%CP composite scaffold	1531:1572	the corresponding 40%CP composite scaffold	1531:1572	However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds.
32856644	6	76	theme	composite	1024:1032	arg1	conducive					1137:1145	conducive	1137:1145	conducive	1137:1145	Compared with the pure PLLA scaffold, the CP composite scaffolds showed significantly superior hydrophilicity and compression performance, and also were more conducive to cell adhesion, proliferation, and up-regulating alkaline phosphate activity and calcium deposition due to the presence of CHWs.
32856644	6	76	theme	composite	1024:1032	arg1	scaffolds					1034:1042	the CP composite scaffolds	1017:1042	the CP composite scaffolds	1017:1042	Compared with the pure PLLA scaffold, the CP composite scaffolds showed significantly superior hydrophilicity and compression performance, and also were more conducive to cell adhesion, proliferation, and up-regulating alkaline phosphate activity and calcium deposition due to the presence of CHWs.
32856644	8	77	theme	scaffold	1565:1572	arg1	performance					1477:1487	compression performance	1465:1487	compression performance	1465:1487	However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds.
32856644	8	77	theme	scaffold	1565:1572	arg1	affinity					1495:1502	cell affinity	1490:1502	cell affinity	1490:1502	However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds.
32856644	8	77	theme	scaffold	1565:1572	arg1	activity					1519:1526	osteogenic activity	1508:1526	osteogenic activity	1508:1526	However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds.
32856644	3	78	theme	composite	736:744	arg1	inks					746:749	CHW/PLLA (CP) composite inks	722:749	CHW/PLLA (CP) composite inks	722:749	Moreover, a trisolvent system, including dichloromethane (DCM), 2-butoxyethanlol (2-Bu) and dibutyl phthalate (DBP), instead of a single solvent system of DCM was chosen to prepare CHW/PLLA (CP) composite inks.
32856644	7	79	theme	%	1397:1397	arg1	range					1381:1385	the range	1377:1385	the range of 0-20 wt%	1377:1397	Moreover, these promoting effects of CHWs are positively related to the content of the whiskers in the range of 0-20 wt%.
32856644	2	80	theme	cell	451:454	arg1	etc.					487:490	etc.	487:490	etc.	487:490	Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix.
32856644	2	80	theme	cell	451:454	arg1	affinity					456:463	excellent cell affinity	441:463	excellent cell affinity	441:463	Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix.
32856644	2	80	theme	cell	451:454	arg1	activity					477:484	osteogenic activity	466:484	osteogenic activity	466:484	Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix.
32856644	0	81	theme	scaffold	73:80	arg1	evaluation					16:25	evaluation	16:25	evaluation	16:25	Fabrication and evaluation of a chitin whisker/poly(L-lactide) composite scaffold by the direct trisolvent-ink writing method for bone tissue engineering.
32856644	0	81	theme	scaffold	73:80	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and evaluation of a chitin whisker/poly(L-lactide) composite scaffold by the direct trisolvent-ink writing method for bone tissue engineering.
32856644	9	82	theme	%	1786:1786	arg1	scaffold					1800:1807	only the 40%CP composite scaffold	1775:1807	only the 40%CP composite scaffold	1775:1807	Interestingly, compared with these scaffolds containing a lower mass content of CHWs, only the 40%CP composite scaffold exhibited significant anti-inflammatory properties.
32856644	3	83	theme	CP	732:733	arg1	inks					746:749	CHW/PLLA (CP) composite inks	722:749	CHW/PLLA (CP) composite inks	722:749	Moreover, a trisolvent system, including dichloromethane (DCM), 2-butoxyethanlol (2-Bu) and dibutyl phthalate (DBP), instead of a single solvent system of DCM was chosen to prepare CHW/PLLA (CP) composite inks.
32856644	5	84	theme	%	976:976	arg1	size					932:935	a pore size	925:935	a pore size of 400 ± 14 μm	925:950	The as-printed CP composite scaffolds have good 3D porous structures with a pore size of 400 ± 14 μm and a porosity of 80 ± 5%.
32856644	5	84	theme	%	976:976	arg1	porosity					958:965	a porosity	956:965	a porosity of 80 ± 5%	956:976	The as-printed CP composite scaffolds have good 3D porous structures with a pore size of 400 ± 14 μm and a porosity of 80 ± 5%.
32856644	4	85	theme	direct	824:829	arg1	method					843:848	the direct ink writing method	820:848	the direct ink writing method	820:848	Then, the CP porous composite scaffolds were further fabricated via the direct ink writing method.
32856644	9	86	theme	composite	1790:1798	arg1	scaffold					1800:1807	only the 40%CP composite scaffold	1775:1807	only the 40%CP composite scaffold	1775:1807	Interestingly, compared with these scaffolds containing a lower mass content of CHWs, only the 40%CP composite scaffold exhibited significant anti-inflammatory properties.
32856644	3	87	theme	CHW/PLLA	722:729	arg1	inks					746:749	CHW/PLLA (CP) composite inks	722:749	CHW/PLLA (CP) composite inks	722:749	Moreover, a trisolvent system, including dichloromethane (DCM), 2-butoxyethanlol (2-Bu) and dibutyl phthalate (DBP), instead of a single solvent system of DCM was chosen to prepare CHW/PLLA (CP) composite inks.
32856644	6	88	theme	alkaline	1198:1205	arg1	activity					1217:1224	up-regulating alkaline phosphate activity	1184:1224	up-regulating alkaline phosphate activity	1184:1224	Compared with the pure PLLA scaffold, the CP composite scaffolds showed significantly superior hydrophilicity and compression performance, and also were more conducive to cell adhesion, proliferation, and up-regulating alkaline phosphate activity and calcium deposition due to the presence of CHWs.
32856644	5	89	theme	as-printed	855:864	arg1	scaffolds					879:887	The as-printed CP composite scaffolds	851:887	The as-printed CP composite scaffolds	851:887	The as-printed CP composite scaffolds have good 3D porous structures with a pore size of 400 ± 14 μm and a porosity of 80 ± 5%.
32856644	4	90	theme	writing	835:841	arg1	method					843:848	the direct ink writing method	820:848	the direct ink writing method	820:848	Then, the CP porous composite scaffolds were further fabricated via the direct ink writing method.
32856644	0	91	theme	trisolvent-ink	96:109	arg1	method					119:124	the direct trisolvent-ink writing method	85:124	the direct trisolvent-ink writing method for bone tissue engineering	85:152	Fabrication and evaluation of a chitin whisker/poly(L-lactide) composite scaffold by the direct trisolvent-ink writing method for bone tissue engineering.
32856644	9	92	theme	anti-inflammatory	1831:1847	arg1	properties					1849:1858	significant anti-inflammatory properties	1819:1858	significant anti-inflammatory properties	1819:1858	Interestingly, compared with these scaffolds containing a lower mass content of CHWs, only the 40%CP composite scaffold exhibited significant anti-inflammatory properties.
32856644	1	93	theme	tissue	352:357	arg1	repair					359:364	bone tissue repair	347:364	bone tissue repair	347:364	Although poly(l-lactide) (PLLA) based porous scaffolds have been widely fabricated through 3D printing, their poor mechanical properties and osteogenic activity still do not meet the needs of bone tissue repair.
32856644	7	94	from	range	1381:1385	arg1	content					1350:1356	the content	1346:1356	the content of the whiskers in the range of 0-20 wt%	1346:1397	Moreover, these promoting effects of CHWs are positively related to the content of the whiskers in the range of 0-20 wt%.
32856644	10	95	theme	tissue	1924:1929	arg1	engineering					1931:1941	bone tissue engineering	1919:1941	bone tissue engineering application	1919:1953	These robust CP composite scaffolds offer a new route for bone tissue engineering application.
32856644	2	96	theme	chitin	375:380	arg1	CHWs					392:395	CHWs	392:395	CHWs	392:395	Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix.
32856644	2	96	theme	chitin	375:380	arg1	whiskers					382:389	chitin whiskers	375:389	chitin whiskers (CHWs)	375:396	Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix.
32856644	0	97	theme	chitin	32:37	arg1	scaffold					73:80	a chitin whisker/poly(L-lactide) composite scaffold	30:80	a chitin whisker/poly(L-lactide) composite scaffold	30:80	Fabrication and evaluation of a chitin whisker/poly(L-lactide) composite scaffold by the direct trisolvent-ink writing method for bone tissue engineering.
32856644	1	98	theme	poor	265:268	arg1	properties					281:290	their poor mechanical properties	259:290	their poor mechanical properties	259:290	Although poly(l-lactide) (PLLA) based porous scaffolds have been widely fabricated through 3D printing, their poor mechanical properties and osteogenic activity still do not meet the needs of bone tissue repair.
32856644	5	99	theme	3D	899:900	arg1	structures					909:918	good 3D porous structures	894:918	good 3D porous structures	894:918	The as-printed CP composite scaffolds have good 3D porous structures with a pore size of 400 ± 14 μm and a porosity of 80 ± 5%.
32856644	2	100	theme	mechanical	418:427	arg1	properties					429:438	outstanding mechanical properties	406:438	outstanding mechanical properties	406:438	Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix.
32856644	6	101	theme	up-regulating	1184:1196	arg1	activity					1217:1224	up-regulating alkaline phosphate activity	1184:1224	up-regulating alkaline phosphate activity	1184:1224	Compared with the pure PLLA scaffold, the CP composite scaffolds showed significantly superior hydrophilicity and compression performance, and also were more conducive to cell adhesion, proliferation, and up-regulating alkaline phosphate activity and calcium deposition due to the presence of CHWs.
32856644	7	102	theme	whiskers	1365:1372	arg1	content					1350:1356	the content	1346:1356	the content of the whiskers in the range of 0-20 wt%	1346:1397	Moreover, these promoting effects of CHWs are positively related to the content of the whiskers in the range of 0-20 wt%.
32856644	8	103	from	scaffolds	1678:1686	arg1	microstructure					1626:1639	the different microstructure	1612:1639	the different microstructure of the scaffold from other composite scaffolds	1612:1686	However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds.
32856644	8	104	theme	wt	1456:1457	arg1	%					1458:1458	40 wt%	1453:1458	40 wt%	1453:1458	However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds.
32856644	1	105	theme	osteogenic	296:305	arg1	activity					307:314	osteogenic activity	296:314	osteogenic activity	296:314	Although poly(l-lactide) (PLLA) based porous scaffolds have been widely fabricated through 3D printing, their poor mechanical properties and osteogenic activity still do not meet the needs of bone tissue repair.
32856644	5	106	theme	composite	869:877	arg1	scaffolds					879:887	The as-printed CP composite scaffolds	851:887	The as-printed CP composite scaffolds	851:887	The as-printed CP composite scaffolds have good 3D porous structures with a pore size of 400 ± 14 μm and a porosity of 80 ± 5%.
32856644	10	107	theme	robust	1867:1872	arg1	scaffolds					1887:1895	These robust CP composite scaffolds	1861:1895	These robust CP composite scaffolds	1861:1895	These robust CP composite scaffolds offer a new route for bone tissue engineering application.
32856644	8	108	theme	compression	1465:1475	arg1	performance					1477:1487	compression performance	1465:1487	compression performance	1465:1487	However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds.
33965543	11	0	theme	dichotomous	1429:1439	arg1	effect					1441:1446	the dichotomous effect	1425:1446	the dichotomous effect by the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets	1425:1598	The findings provide a deeper understanding of the dichotomous effect by the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets.
33965543	9	1	theme	tablet	1196:1201	arg1	properties					1214:1223	the tablet mechanical properties	1192:1223	the tablet mechanical properties	1192:1223	The presence of starch contributed positively to ASA stability although increasing proportions of starch compromised the tablet mechanical properties.
33965543	11	2	from	components	1469:1478	arg1	formulations					1483:1494	formulations	1483:1494	formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets	1483:1598	The findings provide a deeper understanding of the dichotomous effect by the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets.
33965543	11	3	theme	components	1469:1478	arg1	proportion					1455:1464	the proportion	1451:1464	the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets	1451:1598	The findings provide a deeper understanding of the dichotomous effect by the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets.
33965543	11	4	theme	tablets	1592:1598	arg1	stability					1541:1549	drug stability	1536:1549	drug stability	1536:1549	The findings provide a deeper understanding of the dichotomous effect by the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets.
33965543	11	4	theme	tablets	1592:1598	arg1	strength					1566:1573	mechanical strength	1555:1573	mechanical strength of the resultant tablets	1555:1598	The findings provide a deeper understanding of the dichotomous effect by the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets.
33965543	7	5	theme	level	875:879	arg1	importance					848:857	the importance	844:857	the importance of drug loading level on its stability	844:896	The stability study showed the importance of drug loading level on its stability.
33965543	10	6	theme	fibrous	1317:1323	arg1	properties					1325:1334	its plastically deforming and fibrous properties	1287:1334	its plastically deforming and fibrous properties	1287:1334	Contrastingly, MCC produced mechanically stronger tablets as its plastically deforming and fibrous properties contributed to a good structural network.
33965543	7	7	theme	drug	862:865	arg1	level					875:879	drug loading level	862:879	drug loading level	862:879	The stability study showed the importance of drug loading level on its stability.
33965543	11	8	contain	containing	1496:1505	arg2	drug					1528:1531	a moisture sensitive drug	1507:1531	a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets	1507:1598	The findings provide a deeper understanding of the dichotomous effect by the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets.
33965543	11	8	contain	containing	1496:1505	arg1	formulations					1483:1494	formulations	1483:1494	formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets	1483:1598	The findings provide a deeper understanding of the dichotomous effect by the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets.
33965543	5	9	theme	sorption	675:682	arg1	properties					684:693	their moisture sorption properties	660:693	their moisture sorption properties	660:693	The excipients were evaluated for their moisture sorption properties.
33965543	0	10	theme	sensitive	110:118	arg1	drug					120:123	a moisture sensitive drug	99:123	a moisture sensitive drug	99:123	Investigation on the impact of different proportions of components in formulations on stability of a moisture sensitive drug.
33965543	3	11	from	influence	372:380	arg1	formulations					417:428	formulations	417:428	formulations	417:428	Therefore, this study was designed to elucidate the relative influence of the proportion of components in formulations on tablet properties.
33965543	3	11	from	influence	372:380	arg1	properties					440:449	tablet properties	433:449	tablet properties	433:449	Therefore, this study was designed to elucidate the relative influence of the proportion of components in formulations on tablet properties.
33965543	1	12	theme	formulation	203:213	arg1	compositions					215:226	formulation compositions	203:226	formulation compositions	203:226	Physicochemical and mechanical properties of tablets are largely dictated by formulation compositions.
33965543	0	13	from	formulations	70:81	arg1	proportions					41:51	different proportions	31:51	different proportions of components in formulations	31:81	Investigation on the impact of different proportions of components in formulations on stability of a moisture sensitive drug.
33965543	8	14	from	isotherms	1064:1072	arg1	areas					1012:1016	larger areas	1005:1016	larger areas of hysteresis loop in their moisture sorption isotherms	1005:1072	For a fixed ASA proportion, formulations with more starch were able to absorb more moisture and possessed larger areas of hysteresis loop in their moisture sorption isotherms.
33965543	0	15	from	components	56:65	arg1	formulations					70:81	formulations	70:81	formulations	70:81	Investigation on the impact of different proportions of components in formulations on stability of a moisture sensitive drug.
33965543	11	16	theme	deeper	1401:1406	arg1	understanding					1408:1420	a deeper understanding	1399:1420	a deeper understanding of the dichotomous effect by the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets	1399:1598	The findings provide a deeper understanding of the dichotomous effect by the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets.
33965543	2	17	theme	Different	229:237	arg1	excipients					239:248	Different excipients	229:248	Different excipients	229:248	Different excipients possess different tableting and moisture sorption behaviors.
33965543	4	18	theme	Acetylsalicylic	452:466	arg1	ASA					474:476	ASA	474:476	ASA	474:476	Acetylsalicylic acid (ASA) tablets containing different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP) were prepared.
33965543	4	18	theme	Acetylsalicylic	452:466	arg1	acid					468:471	Acetylsalicylic acid	452:471	Acetylsalicylic acid (ASA) tablets containing different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP)	452:609	Acetylsalicylic acid (ASA) tablets containing different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP) were prepared.
33965543	6	19	theme	Mechanical	696:705	arg1	strength					707:714	Mechanical strength	696:714	Mechanical strength of the tablets	696:729	Mechanical strength of the tablets was determined alongside with ASA stability, by storing the tablets at 75% RH, 25 °C.
33965543	2	20	theme	moisture	282:289	arg1	behaviors					300:308	different tableting and moisture sorption behaviors	258:308	different tableting and moisture sorption behaviors	258:308	Different excipients possess different tableting and moisture sorption behaviors.
33965543	6	21	theme	tablets	723:729	arg1	strength					707:714	Mechanical strength	696:714	Mechanical strength of the tablets	696:729	Mechanical strength of the tablets was determined alongside with ASA stability, by storing the tablets at 75% RH, 25 °C.
33965543	11	22	theme	mechanical	1555:1564	arg1	strength					1566:1573	mechanical strength	1555:1573	mechanical strength of the resultant tablets	1555:1598	The findings provide a deeper understanding of the dichotomous effect by the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets.
33965543	8	23	contain	possessed	995:1003	arg2	areas					1012:1016	larger areas	1005:1016	larger areas of hysteresis loop in their moisture sorption isotherms	1005:1072	For a fixed ASA proportion, formulations with more starch were able to absorb more moisture and possessed larger areas of hysteresis loop in their moisture sorption isotherms.
33965543	8	23	contain	possessed	995:1003	arg1	formulations					927:938	formulations	927:938	formulations with more starch	927:955	For a fixed ASA proportion, formulations with more starch were able to absorb more moisture and possessed larger areas of hysteresis loop in their moisture sorption isotherms.
33965543	0	24	theme	components	56:65	arg1	proportions					41:51	different proportions	31:51	different proportions of components in formulations	31:81	Investigation on the impact of different proportions of components in formulations on stability of a moisture sensitive drug.
33965543	2	25	theme	tableting	268:276	arg1	behaviors					300:308	different tableting and moisture sorption behaviors	258:308	different tableting and moisture sorption behaviors	258:308	Different excipients possess different tableting and moisture sorption behaviors.
33965543	1	26	theme	mechanical	146:155	arg1	properties					157:166	Physicochemical and mechanical properties	126:166	Physicochemical and mechanical properties of tablets	126:177	Physicochemical and mechanical properties of tablets are largely dictated by formulation compositions.
33965543	4	27	theme	microcrystalline	531:546	arg1	cellulose					548:556	microcrystalline cellulose	531:556	microcrystalline cellulose (MCC)	531:562	Acetylsalicylic acid (ASA) tablets containing different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP) were prepared.
33965543	4	27	theme	microcrystalline	531:546	arg1	MCC					559:561	MCC	559:561	MCC	559:561	Acetylsalicylic acid (ASA) tablets containing different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP) were prepared.
33965543	11	28	theme	resultant	1582:1590	arg1	tablets					1592:1598	the resultant tablets	1578:1598	the resultant tablets	1578:1598	The findings provide a deeper understanding of the dichotomous effect by the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets.
33965543	8	29	theme	hysteresis	1021:1030	arg1	loop					1032:1035	hysteresis loop	1021:1035	hysteresis loop in their moisture sorption isotherms	1021:1072	For a fixed ASA proportion, formulations with more starch were able to absorb more moisture and possessed larger areas of hysteresis loop in their moisture sorption isotherms.
33965543	0	30	from	Investigation	0:12	arg1	impact					21:26	the impact	17:26	the impact of different proportions of components in formulations on stability of a moisture sensitive drug	17:123	Investigation on the impact of different proportions of components in formulations on stability of a moisture sensitive drug.
33965543	1	31	theme	Physicochemical	126:140	arg1	properties					157:166	Physicochemical and mechanical properties	126:166	Physicochemical and mechanical properties of tablets	126:177	Physicochemical and mechanical properties of tablets are largely dictated by formulation compositions.
33965543	0	32	from	proportions	41:51	arg1	formulations					70:81	formulations	70:81	formulations	70:81	Investigation on the impact of different proportions of components in formulations on stability of a moisture sensitive drug.
33965543	11	33	theme	drug	1536:1539	arg1	stability					1541:1549	drug stability	1536:1549	drug stability	1536:1549	The findings provide a deeper understanding of the dichotomous effect by the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets.
33965543	8	34	theme	moisture	1046:1053	arg1	isotherms					1064:1072	their moisture sorption isotherms	1040:1072	their moisture sorption isotherms	1040:1072	For a fixed ASA proportion, formulations with more starch were able to absorb more moisture and possessed larger areas of hysteresis loop in their moisture sorption isotherms.
33965543	4	35	theme	starch	523:528	arg1	proportions					508:518	different proportions	498:518	different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP)	498:609	Acetylsalicylic acid (ASA) tablets containing different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP) were prepared.
33965543	9	36	theme	starch	1091:1096	arg1	presence					1079:1086	The presence	1075:1086	The presence of starch	1075:1096	The presence of starch contributed positively to ASA stability although increasing proportions of starch compromised the tablet mechanical properties.
33965543	7	37	theme	stability	821:829	arg1	study					831:835	The stability study	817:835	The stability study	817:835	The stability study showed the importance of drug loading level on its stability.
33965543	8	38	theme	fixed	905:909	arg1	proportion					915:924	a fixed ASA proportion	903:924	a fixed ASA proportion	903:924	For a fixed ASA proportion, formulations with more starch were able to absorb more moisture and possessed larger areas of hysteresis loop in their moisture sorption isotherms.
33965543	8	39	with	formulations	927:938	arg1	starch					950:955	more starch	945:955	more starch	945:955	For a fixed ASA proportion, formulations with more starch were able to absorb more moisture and possessed larger areas of hysteresis loop in their moisture sorption isotherms.
33965543	4	40	theme	phosphate	585:593	arg1	dihydrate					595:603	calcium hydrogen phosphate dihydrate	568:603	calcium hydrogen phosphate dihydrate (DCP)	568:609	Acetylsalicylic acid (ASA) tablets containing different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP) were prepared.
33965543	4	40	theme	phosphate	585:593	arg1	DCP					606:608	DCP	606:608	DCP	606:608	Acetylsalicylic acid (ASA) tablets containing different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP) were prepared.
33965543	10	41	theme	structural	1358:1367	arg1	network					1369:1375	a good structural network	1351:1375	a good structural network	1351:1375	Contrastingly, MCC produced mechanically stronger tablets as its plastically deforming and fibrous properties contributed to a good structural network.
33965543	4	42	contain	containing	487:496	arg1	tablets					479:485	Acetylsalicylic acid (ASA) tablets	452:485	Acetylsalicylic acid (ASA) tablets containing different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP)	452:609	Acetylsalicylic acid (ASA) tablets containing different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP) were prepared.
33965543	4	42	contain	containing	487:496	arg2	proportions					508:518	different proportions	498:518	different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP)	498:609	Acetylsalicylic acid (ASA) tablets containing different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP) were prepared.
33965543	11	43	theme	sensitive	1518:1526	arg1	drug					1528:1531	a moisture sensitive drug	1507:1531	a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets	1507:1598	The findings provide a deeper understanding of the dichotomous effect by the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets.
33965543	9	44	theme	ASA	1124:1126	arg1	stability					1128:1136	ASA stability	1124:1136	ASA stability	1124:1136	The presence of starch contributed positively to ASA stability although increasing proportions of starch compromised the tablet mechanical properties.
33965543	7	45	from	importance	848:857	arg1	stability					888:896	its stability	884:896	its stability	884:896	The stability study showed the importance of drug loading level on its stability.
33965543	0	46	theme	proportions	41:51	arg1	impact					21:26	the impact	17:26	the impact of different proportions of components in formulations on stability of a moisture sensitive drug	17:123	Investigation on the impact of different proportions of components in formulations on stability of a moisture sensitive drug.
33965543	0	47	from	impact	21:26	arg1	stability					86:94	stability	86:94	stability of a moisture sensitive drug	86:123	Investigation on the impact of different proportions of components in formulations on stability of a moisture sensitive drug.
33965543	8	48	theme	more	945:948	arg1	starch					950:955	more starch	945:955	more starch	945:955	For a fixed ASA proportion, formulations with more starch were able to absorb more moisture and possessed larger areas of hysteresis loop in their moisture sorption isotherms.
33965543	4	49	theme	calcium	568:574	arg1	dihydrate					595:603	calcium hydrogen phosphate dihydrate	568:603	calcium hydrogen phosphate dihydrate (DCP)	568:609	Acetylsalicylic acid (ASA) tablets containing different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP) were prepared.
33965543	4	49	theme	calcium	568:574	arg1	DCP					606:608	DCP	606:608	DCP	606:608	Acetylsalicylic acid (ASA) tablets containing different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP) were prepared.
33965543	11	50	from	formulations	1483:1494	arg1	proportion					1455:1464	the proportion	1451:1464	the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets	1451:1598	The findings provide a deeper understanding of the dichotomous effect by the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets.
33965543	11	51	theme	effect	1441:1446	arg1	understanding					1408:1420	a deeper understanding	1399:1420	a deeper understanding of the dichotomous effect by the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets	1399:1598	The findings provide a deeper understanding of the dichotomous effect by the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets.
33965543	9	52	theme	starch	1173:1178	arg1	proportions					1158:1168	increasing proportions	1147:1168	increasing proportions of starch	1147:1178	The presence of starch contributed positively to ASA stability although increasing proportions of starch compromised the tablet mechanical properties.
33965543	2	53	contain	possess	250:256	arg2	behaviors					300:308	different tableting and moisture sorption behaviors	258:308	different tableting and moisture sorption behaviors	258:308	Different excipients possess different tableting and moisture sorption behaviors.
33965543	2	53	contain	possess	250:256	arg1	excipients					239:248	Different excipients	229:248	Different excipients	229:248	Different excipients possess different tableting and moisture sorption behaviors.
33965543	8	54	from	areas	1012:1016	arg1	isotherms					1064:1072	their moisture sorption isotherms	1040:1072	their moisture sorption isotherms	1040:1072	For a fixed ASA proportion, formulations with more starch were able to absorb more moisture and possessed larger areas of hysteresis loop in their moisture sorption isotherms.
33965543	8	55	theme	more	977:980	arg1	moisture					982:989	more moisture	977:989	more moisture	977:989	For a fixed ASA proportion, formulations with more starch were able to absorb more moisture and possessed larger areas of hysteresis loop in their moisture sorption isotherms.
33965543	9	56	theme	mechanical	1203:1212	arg1	properties					1214:1223	the tablet mechanical properties	1192:1223	the tablet mechanical properties	1192:1223	The presence of starch contributed positively to ASA stability although increasing proportions of starch compromised the tablet mechanical properties.
33965543	10	57	theme	deforming	1303:1311	arg1	properties					1325:1334	its plastically deforming and fibrous properties	1287:1334	its plastically deforming and fibrous properties	1287:1334	Contrastingly, MCC produced mechanically stronger tablets as its plastically deforming and fibrous properties contributed to a good structural network.
33965543	1	58	theme	tablets	171:177	arg1	properties					157:166	Physicochemical and mechanical properties	126:166	Physicochemical and mechanical properties of tablets	126:177	Physicochemical and mechanical properties of tablets are largely dictated by formulation compositions.
33965543	8	59	theme	larger	1005:1010	arg1	areas					1012:1016	larger areas	1005:1016	larger areas of hysteresis loop in their moisture sorption isotherms	1005:1072	For a fixed ASA proportion, formulations with more starch were able to absorb more moisture and possessed larger areas of hysteresis loop in their moisture sorption isotherms.
33965543	5	60	theme	moisture	666:673	arg1	properties					684:693	their moisture sorption properties	660:693	their moisture sorption properties	660:693	The excipients were evaluated for their moisture sorption properties.
33965543	7	61	theme	loading	867:873	arg1	level					875:879	drug loading level	862:879	drug loading level	862:879	The stability study showed the importance of drug loading level on its stability.
33965543	0	62	theme	drug	120:123	arg1	stability					86:94	stability	86:94	stability of a moisture sensitive drug	86:123	Investigation on the impact of different proportions of components in formulations on stability of a moisture sensitive drug.
33965543	8	63	from	loop	1032:1035	arg1	isotherms					1064:1072	their moisture sorption isotherms	1040:1072	their moisture sorption isotherms	1040:1072	For a fixed ASA proportion, formulations with more starch were able to absorb more moisture and possessed larger areas of hysteresis loop in their moisture sorption isotherms.
33965543	11	64	from	proportion	1455:1464	arg1	formulations					1483:1494	formulations	1483:1494	formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets	1483:1598	The findings provide a deeper understanding of the dichotomous effect by the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets.
33965543	2	65	theme	different	258:266	arg1	behaviors					300:308	different tableting and moisture sorption behaviors	258:308	different tableting and moisture sorption behaviors	258:308	Different excipients possess different tableting and moisture sorption behaviors.
33965543	4	66	theme	different	498:506	arg1	proportions					508:518	different proportions	498:518	different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP)	498:609	Acetylsalicylic acid (ASA) tablets containing different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP) were prepared.
33965543	3	67	from	proportion	389:398	arg1	formulations					417:428	formulations	417:428	formulations	417:428	Therefore, this study was designed to elucidate the relative influence of the proportion of components in formulations on tablet properties.
33965543	4	68	theme	acid	468:471	arg1	tablets					479:485	Acetylsalicylic acid (ASA) tablets	452:485	Acetylsalicylic acid (ASA) tablets containing different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP)	452:609	Acetylsalicylic acid (ASA) tablets containing different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP) were prepared.
33965543	2	69	theme	sorption	291:298	arg1	behaviors					300:308	different tableting and moisture sorption behaviors	258:308	different tableting and moisture sorption behaviors	258:308	Different excipients possess different tableting and moisture sorption behaviors.
33965543	10	70	theme	stronger	1267:1274	arg1	tablets					1276:1282	mechanically stronger tablets	1254:1282	mechanically stronger tablets	1254:1282	Contrastingly, MCC produced mechanically stronger tablets as its plastically deforming and fibrous properties contributed to a good structural network.
33965543	3	71	theme	relative	363:370	arg1	influence					372:380	the relative influence	359:380	the relative influence of the proportion of components in formulations on tablet properties	359:449	Therefore, this study was designed to elucidate the relative influence of the proportion of components in formulations on tablet properties.
33965543	4	72	theme	cellulose	548:556	arg1	proportions					508:518	different proportions	498:518	different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP)	498:609	Acetylsalicylic acid (ASA) tablets containing different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP) were prepared.
33965543	8	73	theme	loop	1032:1035	arg1	areas					1012:1016	larger areas	1005:1016	larger areas of hysteresis loop in their moisture sorption isotherms	1005:1072	For a fixed ASA proportion, formulations with more starch were able to absorb more moisture and possessed larger areas of hysteresis loop in their moisture sorption isotherms.
33965543	3	74	theme	proportion	389:398	arg1	influence					372:380	the relative influence	359:380	the relative influence of the proportion of components in formulations on tablet properties	359:449	Therefore, this study was designed to elucidate the relative influence of the proportion of components in formulations on tablet properties.
33965543	6	75	theme	ASA	761:763	arg1	stability					765:773	ASA stability	761:773	ASA stability	761:773	Mechanical strength of the tablets was determined alongside with ASA stability, by storing the tablets at 75% RH, 25 °C.
33965543	0	76	theme	moisture	101:108	arg1	drug					120:123	a moisture sensitive drug	99:123	a moisture sensitive drug	99:123	Investigation on the impact of different proportions of components in formulations on stability of a moisture sensitive drug.
33965543	8	77	theme	sorption	1055:1062	arg1	isotherms					1064:1072	their moisture sorption isotherms	1040:1072	their moisture sorption isotherms	1040:1072	For a fixed ASA proportion, formulations with more starch were able to absorb more moisture and possessed larger areas of hysteresis loop in their moisture sorption isotherms.
33965543	3	78	from	formulations	417:428	arg1	influence					372:380	the relative influence	359:380	the relative influence of the proportion of components in formulations on tablet properties	359:449	Therefore, this study was designed to elucidate the relative influence of the proportion of components in formulations on tablet properties.
33965543	4	79	theme	dihydrate	595:603	arg1	proportions					508:518	different proportions	498:518	different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP)	498:609	Acetylsalicylic acid (ASA) tablets containing different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP) were prepared.
33965543	3	80	theme	components	403:412	arg1	proportion					389:398	the proportion	385:398	the proportion of components in formulations	385:428	Therefore, this study was designed to elucidate the relative influence of the proportion of components in formulations on tablet properties.
33965543	10	81	theme	good	1353:1356	arg1	network					1369:1375	a good structural network	1351:1375	a good structural network	1351:1375	Contrastingly, MCC produced mechanically stronger tablets as its plastically deforming and fibrous properties contributed to a good structural network.
33965543	0	82	theme	different	31:39	arg1	proportions					41:51	different proportions	31:51	different proportions of components in formulations	31:81	Investigation on the impact of different proportions of components in formulations on stability of a moisture sensitive drug.
33965543	8	83	theme	ASA	911:913	arg1	proportion					915:924	a fixed ASA proportion	903:924	a fixed ASA proportion	903:924	For a fixed ASA proportion, formulations with more starch were able to absorb more moisture and possessed larger areas of hysteresis loop in their moisture sorption isotherms.
33965543	11	84	from	drug	1528:1531	arg1	stability					1541:1549	drug stability	1536:1549	drug stability	1536:1549	The findings provide a deeper understanding of the dichotomous effect by the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets.
33965543	11	84	from	drug	1528:1531	arg1	strength					1566:1573	mechanical strength	1555:1573	mechanical strength of the resultant tablets	1555:1598	The findings provide a deeper understanding of the dichotomous effect by the proportion of components in formulations containing a moisture sensitive drug on drug stability and mechanical strength of the resultant tablets.
33965543	3	85	theme	tablet	433:438	arg1	properties					440:449	tablet properties	433:449	tablet properties	433:449	Therefore, this study was designed to elucidate the relative influence of the proportion of components in formulations on tablet properties.
33965543	4	86	theme	hydrogen	576:583	arg1	dihydrate					595:603	calcium hydrogen phosphate dihydrate	568:603	calcium hydrogen phosphate dihydrate (DCP)	568:609	Acetylsalicylic acid (ASA) tablets containing different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP) were prepared.
33965543	4	86	theme	hydrogen	576:583	arg1	DCP					606:608	DCP	606:608	DCP	606:608	Acetylsalicylic acid (ASA) tablets containing different proportions of starch, microcrystalline cellulose (MCC) and calcium hydrogen phosphate dihydrate (DCP) were prepared.
33965543	9	87	theme	increasing	1147:1156	arg1	proportions					1158:1168	increasing proportions	1147:1168	increasing proportions of starch	1147:1178	The presence of starch contributed positively to ASA stability although increasing proportions of starch compromised the tablet mechanical properties.
32614709	0	0	theme	dual	95:98	arg1	delivery					105:112	controlled dual drug delivery	84:112	controlled dual drug delivery	84:112	A dual pH and redox-responsive Ag/AgO/carboxymethyl chitosan composite hydrogel for controlled dual drug delivery.
32614709	2	1	from	ring-opened	488:498	arg1	atmosphere					506:515	Ar atmosphere	503:515	Ar atmosphere	503:515	Dithiodipropionic acid and poly(ethylene glycol) (PEG) were esterified to synthesize a hydrophilic segment PEG-SS-COOH polymer containing a disulfide bond, and ε-caprolactone is ring-opened in Ar atmosphere to obtain a hydrophobic segment HO-PCL-OH homopolymer.
32614709	4	2	theme	Ag/AgO/CMCS	732:742	arg1	hydrogels					754:762	Ag/AgO/CMCS composite hydrogels	732:762	Ag/AgO/CMCS composite hydrogels with a pH and redox dual-responsive	732:798	Ag/AgO/CMCS composite hydrogels with a pH and redox dual-responsive were prepared by dispersing aspirin-loaded micelles into an Ag/AgO/CMCS hydrogel network loaded with another hydrophilic drug ciprofloxacin hydrochloride (CIP).
32614709	0	3	theme	controlled	84:93	arg1	delivery					105:112	controlled dual drug delivery	84:112	controlled dual drug delivery	84:112	A dual pH and redox-responsive Ag/AgO/carboxymethyl chitosan composite hydrogel for controlled dual drug delivery.
32614709	3	4	theme	aqueous	714:720	arg1	solution					722:729	an aqueous solution	711:729	an aqueous solution	711:729	The PEG-SS-PCL-SS-PEG triblock polymers were prepared by reacting the two in a certain ratio, and spontaneously assembled into micelles in an aqueous solution.
32614709	4	5	theme	ciprofloxacin	926:938	arg1	hydrochloride					940:952	another hydrophilic drug ciprofloxacin hydrochloride	901:952	another hydrophilic drug ciprofloxacin hydrochloride (CIP)	901:958	Ag/AgO/CMCS composite hydrogels with a pH and redox dual-responsive were prepared by dispersing aspirin-loaded micelles into an Ag/AgO/CMCS hydrogel network loaded with another hydrophilic drug ciprofloxacin hydrochloride (CIP).
32614709	4	5	theme	ciprofloxacin	926:938	arg1	CIP					955:957	CIP	955:957	CIP	955:957	Ag/AgO/CMCS composite hydrogels with a pH and redox dual-responsive were prepared by dispersing aspirin-loaded micelles into an Ag/AgO/CMCS hydrogel network loaded with another hydrophilic drug ciprofloxacin hydrochloride (CIP).
32614709	6	6	theme	high	1391:1394	arg1	concentration					1400:1412	high DTT concentration	1391:1412	high DTT concentration	1391:1412	In vitro simulated drug release test of composite hydrogel showed that the hydrogel network swelled or degraded, the micelles were lysed, and the release of the drug was triggered when the composite hydrogels were exposed to the micro-environment with high pH or high DTT concentration.
32614709	6	7	theme	drug	1147:1150	arg1	test					1160:1163	In vitro simulated drug release test	1128:1163	In vitro simulated drug release test of composite hydrogel	1128:1185	In vitro simulated drug release test of composite hydrogel showed that the hydrogel network swelled or degraded, the micelles were lysed, and the release of the drug was triggered when the composite hydrogels were exposed to the micro-environment with high pH or high DTT concentration.
32614709	2	8	theme	HO-PCL-OH	549:557	arg1	homopolymer					559:569	a hydrophobic segment HO-PCL-OH homopolymer	527:569	a hydrophobic segment HO-PCL-OH homopolymer	527:569	Dithiodipropionic acid and poly(ethylene glycol) (PEG) were esterified to synthesize a hydrophilic segment PEG-SS-COOH polymer containing a disulfide bond, and ε-caprolactone is ring-opened in Ar atmosphere to obtain a hydrophobic segment HO-PCL-OH homopolymer.
32614709	4	9	theme	aspirin-loaded	828:841	arg1	micelles					843:850	aspirin-loaded micelles	828:850	aspirin-loaded micelles	828:850	Ag/AgO/CMCS composite hydrogels with a pH and redox dual-responsive were prepared by dispersing aspirin-loaded micelles into an Ag/AgO/CMCS hydrogel network loaded with another hydrophilic drug ciprofloxacin hydrochloride (CIP).
32614709	6	10	theme	drug	1289:1292	arg1	release					1274:1280	the release	1270:1280	the release of the drug	1270:1292	In vitro simulated drug release test of composite hydrogel showed that the hydrogel network swelled or degraded, the micelles were lysed, and the release of the drug was triggered when the composite hydrogels were exposed to the micro-environment with high pH or high DTT concentration.
32614709	5	11	theme	bonds	1054:1058	arg1	insertion					1031:1039	the insertion	1027:1039	the insertion of disulfide bonds	1027:1058	The results showed that the micelles had redox sensitivity due to the insertion of disulfide bonds, and had certain stability within 24 h without reducing substances.
32614709	1	12	theme	hydrogel	280:287	arg1	complexes					299:307	hydrogel inclusion complexes	280:307	hydrogel inclusion complexes	280:307	A pH and redox dual-responsive composite hydrogel was developed, which was formed from triblock copolymer PEG-SS-PCL-SS-PEG and Ag/AgO/carboxymethyl chitosan (CMCS) hydrogel inclusion complexes.
32614709	0	13	theme	drug	100:103	arg1	delivery					105:112	controlled dual drug delivery	84:112	controlled dual drug delivery	84:112	A dual pH and redox-responsive Ag/AgO/carboxymethyl chitosan composite hydrogel for controlled dual drug delivery.
32614709	3	14	theme	PEG-SS-PCL-SS-PEG	576:592	arg1	polymers					603:610	The PEG-SS-PCL-SS-PEG triblock polymers	572:610	The PEG-SS-PCL-SS-PEG triblock polymers	572:610	The PEG-SS-PCL-SS-PEG triblock polymers were prepared by reacting the two in a certain ratio, and spontaneously assembled into micelles in an aqueous solution.
32614709	2	15	theme	Dithiodipropionic	310:326	arg1	acid					328:331	Dithiodipropionic acid	310:331	Dithiodipropionic acid	310:331	Dithiodipropionic acid and poly(ethylene glycol) (PEG) were esterified to synthesize a hydrophilic segment PEG-SS-COOH polymer containing a disulfide bond, and ε-caprolactone is ring-opened in Ar atmosphere to obtain a hydrophobic segment HO-PCL-OH homopolymer.
32614709	1	16	theme	inclusion	289:297	arg1	complexes					299:307	hydrogel inclusion complexes	280:307	hydrogel inclusion complexes	280:307	A pH and redox dual-responsive composite hydrogel was developed, which was formed from triblock copolymer PEG-SS-PCL-SS-PEG and Ag/AgO/carboxymethyl chitosan (CMCS) hydrogel inclusion complexes.
32614709	2	17	theme	hydrophobic	529:539	arg1	segment					541:547	a hydrophobic segment	527:547	a hydrophobic segment HO-PCL-OH homopolymer	527:569	Dithiodipropionic acid and poly(ethylene glycol) (PEG) were esterified to synthesize a hydrophilic segment PEG-SS-COOH polymer containing a disulfide bond, and ε-caprolactone is ring-opened in Ar atmosphere to obtain a hydrophobic segment HO-PCL-OH homopolymer.
32614709	6	18	theme	release	1152:1158	arg1	test					1160:1163	In vitro simulated drug release test	1128:1163	In vitro simulated drug release test of composite hydrogel	1128:1185	In vitro simulated drug release test of composite hydrogel showed that the hydrogel network swelled or degraded, the micelles were lysed, and the release of the drug was triggered when the composite hydrogels were exposed to the micro-environment with high pH or high DTT concentration.
32614709	6	19	with	micro-environment	1357:1373	arg1	concentration					1400:1412	high DTT concentration	1391:1412	high DTT concentration	1391:1412	In vitro simulated drug release test of composite hydrogel showed that the hydrogel network swelled or degraded, the micelles were lysed, and the release of the drug was triggered when the composite hydrogels were exposed to the micro-environment with high pH or high DTT concentration.
32614709	6	19	with	micro-environment	1357:1373	arg1	pH					1385:1386	high pH	1380:1386	high pH	1380:1386	In vitro simulated drug release test of composite hydrogel showed that the hydrogel network swelled or degraded, the micelles were lysed, and the release of the drug was triggered when the composite hydrogels were exposed to the micro-environment with high pH or high DTT concentration.
32614709	2	20	theme	segment	409:415	arg1	polymer					429:435	a hydrophilic segment PEG-SS-COOH polymer	395:435	a hydrophilic segment PEG-SS-COOH polymer containing a disulfide bond	395:463	Dithiodipropionic acid and poly(ethylene glycol) (PEG) were esterified to synthesize a hydrophilic segment PEG-SS-COOH polymer containing a disulfide bond, and ε-caprolactone is ring-opened in Ar atmosphere to obtain a hydrophobic segment HO-PCL-OH homopolymer.
32614709	7	21	theme	candidate	1482:1490	arg1	materials					1492:1500	the candidate materials	1478:1500	the candidate materials	1478:1500	Therefore, this composite hydrogel could potentially be one of the candidate materials for the dual drug delivery system.
32614709	6	22	theme	hydrogel	1178:1185	arg1	test					1160:1163	In vitro simulated drug release test	1128:1163	In vitro simulated drug release test of composite hydrogel	1128:1185	In vitro simulated drug release test of composite hydrogel showed that the hydrogel network swelled or degraded, the micelles were lysed, and the release of the drug was triggered when the composite hydrogels were exposed to the micro-environment with high pH or high DTT concentration.
32614709	7	23	theme	materials	1492:1500	arg1	one					1471:1473	one	1471:1473	one	1471:1473	Therefore, this composite hydrogel could potentially be one of the candidate materials for the dual drug delivery system.
32614709	7	23	theme	materials	1492:1500	arg1	materials					1492:1500	the candidate materials	1478:1500	the candidate materials	1478:1500	Therefore, this composite hydrogel could potentially be one of the candidate materials for the dual drug delivery system.
32614709	2	24	theme	hydrophilic	397:407	arg1	polymer					429:435	a hydrophilic segment PEG-SS-COOH polymer	395:435	a hydrophilic segment PEG-SS-COOH polymer containing a disulfide bond	395:463	Dithiodipropionic acid and poly(ethylene glycol) (PEG) were esterified to synthesize a hydrophilic segment PEG-SS-COOH polymer containing a disulfide bond, and ε-caprolactone is ring-opened in Ar atmosphere to obtain a hydrophobic segment HO-PCL-OH homopolymer.
32614709	0	25	theme	dual	2:5	arg1	pH					7:8	A dual pH	0:8	A dual pH	0:8	A dual pH and redox-responsive Ag/AgO/carboxymethyl chitosan composite hydrogel for controlled dual drug delivery.
32614709	2	26	theme	Ar	503:504	arg1	atmosphere					506:515	Ar atmosphere	503:515	Ar atmosphere	503:515	Dithiodipropionic acid and poly(ethylene glycol) (PEG) were esterified to synthesize a hydrophilic segment PEG-SS-COOH polymer containing a disulfide bond, and ε-caprolactone is ring-opened in Ar atmosphere to obtain a hydrophobic segment HO-PCL-OH homopolymer.
32614709	4	27	theme	drug	921:924	arg1	hydrochloride					940:952	another hydrophilic drug ciprofloxacin hydrochloride	901:952	another hydrophilic drug ciprofloxacin hydrochloride (CIP)	901:958	Ag/AgO/CMCS composite hydrogels with a pH and redox dual-responsive were prepared by dispersing aspirin-loaded micelles into an Ag/AgO/CMCS hydrogel network loaded with another hydrophilic drug ciprofloxacin hydrochloride (CIP).
32614709	4	27	theme	drug	921:924	arg1	CIP					955:957	CIP	955:957	CIP	955:957	Ag/AgO/CMCS composite hydrogels with a pH and redox dual-responsive were prepared by dispersing aspirin-loaded micelles into an Ag/AgO/CMCS hydrogel network loaded with another hydrophilic drug ciprofloxacin hydrochloride (CIP).
32614709	2	28	theme	ethylene	342:349	arg1	poly					337:340	poly	337:340	poly(ethylene glycol) (PEG)	337:363	Dithiodipropionic acid and poly(ethylene glycol) (PEG) were esterified to synthesize a hydrophilic segment PEG-SS-COOH polymer containing a disulfide bond, and ε-caprolactone is ring-opened in Ar atmosphere to obtain a hydrophobic segment HO-PCL-OH homopolymer.
32614709	2	28	theme	ethylene	342:349	arg1	glycol					351:356	ethylene glycol	342:356	ethylene glycol	342:356	Dithiodipropionic acid and poly(ethylene glycol) (PEG) were esterified to synthesize a hydrophilic segment PEG-SS-COOH polymer containing a disulfide bond, and ε-caprolactone is ring-opened in Ar atmosphere to obtain a hydrophobic segment HO-PCL-OH homopolymer.
32614709	6	29	theme	simulated	1137:1145	arg1	test					1160:1163	In vitro simulated drug release test	1128:1163	In vitro simulated drug release test of composite hydrogel	1128:1185	In vitro simulated drug release test of composite hydrogel showed that the hydrogel network swelled or degraded, the micelles were lysed, and the release of the drug was triggered when the composite hydrogels were exposed to the micro-environment with high pH or high DTT concentration.
32614709	6	30	theme	In	1128:1129	arg1	test					1160:1163	In vitro simulated drug release test	1128:1163	In vitro simulated drug release test of composite hydrogel	1128:1185	In vitro simulated drug release test of composite hydrogel showed that the hydrogel network swelled or degraded, the micelles were lysed, and the release of the drug was triggered when the composite hydrogels were exposed to the micro-environment with high pH or high DTT concentration.
32614709	4	31	with	hydrogels	754:762	arg1	dual-responsive					784:798	a pH and redox dual-responsive	769:798	dual-responsive	784:798	Ag/AgO/CMCS composite hydrogels with a pH and redox dual-responsive were prepared by dispersing aspirin-loaded micelles into an Ag/AgO/CMCS hydrogel network loaded with another hydrophilic drug ciprofloxacin hydrochloride (CIP).
32614709	4	32	theme	hydrophilic	909:919	arg1	hydrochloride					940:952	another hydrophilic drug ciprofloxacin hydrochloride	901:952	another hydrophilic drug ciprofloxacin hydrochloride (CIP)	901:958	Ag/AgO/CMCS composite hydrogels with a pH and redox dual-responsive were prepared by dispersing aspirin-loaded micelles into an Ag/AgO/CMCS hydrogel network loaded with another hydrophilic drug ciprofloxacin hydrochloride (CIP).
32614709	4	32	theme	hydrophilic	909:919	arg1	CIP					955:957	CIP	955:957	CIP	955:957	Ag/AgO/CMCS composite hydrogels with a pH and redox dual-responsive were prepared by dispersing aspirin-loaded micelles into an Ag/AgO/CMCS hydrogel network loaded with another hydrophilic drug ciprofloxacin hydrochloride (CIP).
32614709	4	33	theme	pH	771:772	arg1	dual-responsive					784:798	a pH and redox dual-responsive	769:798	dual-responsive	784:798	Ag/AgO/CMCS composite hydrogels with a pH and redox dual-responsive were prepared by dispersing aspirin-loaded micelles into an Ag/AgO/CMCS hydrogel network loaded with another hydrophilic drug ciprofloxacin hydrochloride (CIP).
32614709	6	34	theme	high	1380:1383	arg1	pH					1385:1386	high pH	1380:1386	high pH	1380:1386	In vitro simulated drug release test of composite hydrogel showed that the hydrogel network swelled or degraded, the micelles were lysed, and the release of the drug was triggered when the composite hydrogels were exposed to the micro-environment with high pH or high DTT concentration.
32614709	6	35	theme	composite	1317:1325	arg1	hydrogels					1327:1335	the composite hydrogels	1313:1335	the composite hydrogels	1313:1335	In vitro simulated drug release test of composite hydrogel showed that the hydrogel network swelled or degraded, the micelles were lysed, and the release of the drug was triggered when the composite hydrogels were exposed to the micro-environment with high pH or high DTT concentration.
32614709	3	36	theme	triblock	594:601	arg1	polymers					603:610	The PEG-SS-PCL-SS-PEG triblock polymers	572:610	The PEG-SS-PCL-SS-PEG triblock polymers	572:610	The PEG-SS-PCL-SS-PEG triblock polymers were prepared by reacting the two in a certain ratio, and spontaneously assembled into micelles in an aqueous solution.
32614709	2	37	theme	disulfide	450:458	arg1	bond					460:463	a disulfide bond	448:463	a disulfide bond	448:463	Dithiodipropionic acid and poly(ethylene glycol) (PEG) were esterified to synthesize a hydrophilic segment PEG-SS-COOH polymer containing a disulfide bond, and ε-caprolactone is ring-opened in Ar atmosphere to obtain a hydrophobic segment HO-PCL-OH homopolymer.
32614709	5	38	theme	redox	1002:1006	arg1	sensitivity					1008:1018	redox sensitivity	1002:1018	redox sensitivity	1002:1018	The results showed that the micelles had redox sensitivity due to the insertion of disulfide bonds, and had certain stability within 24 h without reducing substances.
32614709	2	39	from	atmosphere	506:515	arg1	ring-opened					488:498	ring-opened	488:498	ring-opened	488:498	Dithiodipropionic acid and poly(ethylene glycol) (PEG) were esterified to synthesize a hydrophilic segment PEG-SS-COOH polymer containing a disulfide bond, and ε-caprolactone is ring-opened in Ar atmosphere to obtain a hydrophobic segment HO-PCL-OH homopolymer.
32614709	4	40	theme	redox	778:782	arg1	dual-responsive					784:798	a pH and redox dual-responsive	769:798	dual-responsive	784:798	Ag/AgO/CMCS composite hydrogels with a pH and redox dual-responsive were prepared by dispersing aspirin-loaded micelles into an Ag/AgO/CMCS hydrogel network loaded with another hydrophilic drug ciprofloxacin hydrochloride (CIP).
32614709	6	41	theme	DTT	1396:1398	arg1	concentration					1400:1412	high DTT concentration	1391:1412	high DTT concentration	1391:1412	In vitro simulated drug release test of composite hydrogel showed that the hydrogel network swelled or degraded, the micelles were lysed, and the release of the drug was triggered when the composite hydrogels were exposed to the micro-environment with high pH or high DTT concentration.
32614709	1	42	theme	triblock	202:209	arg1	PEG-SS-PCL-SS-PEG					221:237	triblock copolymer PEG-SS-PCL-SS-PEG	202:237	triblock copolymer PEG-SS-PCL-SS-PEG	202:237	A pH and redox dual-responsive composite hydrogel was developed, which was formed from triblock copolymer PEG-SS-PCL-SS-PEG and Ag/AgO/carboxymethyl chitosan (CMCS) hydrogel inclusion complexes.
32614709	0	43	theme	Ag/AgO/carboxymethyl	31:50	arg1	hydrogel					71:78	redox-responsive Ag/AgO/carboxymethyl chitosan composite hydrogel	14:78	redox-responsive Ag/AgO/carboxymethyl chitosan composite hydrogel	14:78	A dual pH and redox-responsive Ag/AgO/carboxymethyl chitosan composite hydrogel for controlled dual drug delivery.
32614709	4	44	theme	hydrogel	872:879	arg1	network					881:887	an Ag/AgO/CMCS hydrogel network	857:887	an Ag/AgO/CMCS hydrogel network loaded with another hydrophilic drug ciprofloxacin hydrochloride (CIP)	857:958	Ag/AgO/CMCS composite hydrogels with a pH and redox dual-responsive were prepared by dispersing aspirin-loaded micelles into an Ag/AgO/CMCS hydrogel network loaded with another hydrophilic drug ciprofloxacin hydrochloride (CIP).
32614709	5	45	contain	had	998:1000	arg2	sensitivity					1008:1018	redox sensitivity	1002:1018	redox sensitivity	1002:1018	The results showed that the micelles had redox sensitivity due to the insertion of disulfide bonds, and had certain stability within 24 h without reducing substances.
32614709	5	45	contain	had	998:1000	arg1	micelles					989:996	the micelles	985:996	the micelles	985:996	The results showed that the micelles had redox sensitivity due to the insertion of disulfide bonds, and had certain stability within 24 h without reducing substances.
32614709	1	46	theme	copolymer	211:219	arg1	PEG-SS-PCL-SS-PEG					221:237	triblock copolymer PEG-SS-PCL-SS-PEG	202:237	triblock copolymer PEG-SS-PCL-SS-PEG	202:237	A pH and redox dual-responsive composite hydrogel was developed, which was formed from triblock copolymer PEG-SS-PCL-SS-PEG and Ag/AgO/carboxymethyl chitosan (CMCS) hydrogel inclusion complexes.
32614709	0	47	theme	redox-responsive	14:29	arg1	hydrogel					71:78	redox-responsive Ag/AgO/carboxymethyl chitosan composite hydrogel	14:78	redox-responsive Ag/AgO/carboxymethyl chitosan composite hydrogel	14:78	A dual pH and redox-responsive Ag/AgO/carboxymethyl chitosan composite hydrogel for controlled dual drug delivery.
32614709	7	48	theme	dual	1510:1513	arg1	system					1529:1534	the dual drug delivery system	1506:1534	the dual drug delivery system	1506:1534	Therefore, this composite hydrogel could potentially be one of the candidate materials for the dual drug delivery system.
32614709	7	49	theme	composite	1431:1439	arg1	hydrogel					1441:1448	this composite hydrogel	1426:1448	this composite hydrogel	1426:1448	Therefore, this composite hydrogel could potentially be one of the candidate materials for the dual drug delivery system.
32614709	4	50	theme	Ag/AgO/CMCS	860:870	arg1	network					881:887	an Ag/AgO/CMCS hydrogel network	857:887	an Ag/AgO/CMCS hydrogel network loaded with another hydrophilic drug ciprofloxacin hydrochloride (CIP)	857:958	Ag/AgO/CMCS composite hydrogels with a pH and redox dual-responsive were prepared by dispersing aspirin-loaded micelles into an Ag/AgO/CMCS hydrogel network loaded with another hydrophilic drug ciprofloxacin hydrochloride (CIP).
32614709	2	51	theme	PEG-SS-COOH	417:427	arg1	polymer					429:435	a hydrophilic segment PEG-SS-COOH polymer	395:435	a hydrophilic segment PEG-SS-COOH polymer containing a disulfide bond	395:463	Dithiodipropionic acid and poly(ethylene glycol) (PEG) were esterified to synthesize a hydrophilic segment PEG-SS-COOH polymer containing a disulfide bond, and ε-caprolactone is ring-opened in Ar atmosphere to obtain a hydrophobic segment HO-PCL-OH homopolymer.
32614709	0	52	theme	composite	61:69	arg1	hydrogel					71:78	redox-responsive Ag/AgO/carboxymethyl chitosan composite hydrogel	14:78	redox-responsive Ag/AgO/carboxymethyl chitosan composite hydrogel	14:78	A dual pH and redox-responsive Ag/AgO/carboxymethyl chitosan composite hydrogel for controlled dual drug delivery.
32614709	0	53	theme	chitosan	52:59	arg1	hydrogel					71:78	redox-responsive Ag/AgO/carboxymethyl chitosan composite hydrogel	14:78	redox-responsive Ag/AgO/carboxymethyl chitosan composite hydrogel	14:78	A dual pH and redox-responsive Ag/AgO/carboxymethyl chitosan composite hydrogel for controlled dual drug delivery.
32614709	6	54	dep	In	1128:1129	arg1	vitro					1131:1135	vitro	1131:1135	vitro	1131:1135	In vitro simulated drug release test of composite hydrogel showed that the hydrogel network swelled or degraded, the micelles were lysed, and the release of the drug was triggered when the composite hydrogels were exposed to the micro-environment with high pH or high DTT concentration.
32614709	4	55	theme	composite	744:752	arg1	hydrogels					754:762	Ag/AgO/CMCS composite hydrogels	732:762	Ag/AgO/CMCS composite hydrogels with a pH and redox dual-responsive	732:798	Ag/AgO/CMCS composite hydrogels with a pH and redox dual-responsive were prepared by dispersing aspirin-loaded micelles into an Ag/AgO/CMCS hydrogel network loaded with another hydrophilic drug ciprofloxacin hydrochloride (CIP).
32614709	7	56	theme	drug	1515:1518	arg1	system					1529:1534	the dual drug delivery system	1506:1534	the dual drug delivery system	1506:1534	Therefore, this composite hydrogel could potentially be one of the candidate materials for the dual drug delivery system.
32614709	5	57	theme	disulfide	1044:1052	arg1	bonds					1054:1058	disulfide bonds	1044:1058	disulfide bonds	1044:1058	The results showed that the micelles had redox sensitivity due to the insertion of disulfide bonds, and had certain stability within 24 h without reducing substances.
32614709	3	58	theme	certain	651:657	arg1	ratio					659:663	a certain ratio	649:663	a certain ratio	649:663	The PEG-SS-PCL-SS-PEG triblock polymers were prepared by reacting the two in a certain ratio, and spontaneously assembled into micelles in an aqueous solution.
32614709	5	59	theme	certain	1069:1075	arg1	stability					1077:1085	certain stability	1069:1085	certain stability	1069:1085	The results showed that the micelles had redox sensitivity due to the insertion of disulfide bonds, and had certain stability within 24 h without reducing substances.
32614709	1	60	theme	redox	124:128	arg1	hydrogel					156:163	redox dual-responsive composite hydrogel	124:163	redox dual-responsive composite hydrogel	124:163	A pH and redox dual-responsive composite hydrogel was developed, which was formed from triblock copolymer PEG-SS-PCL-SS-PEG and Ag/AgO/carboxymethyl chitosan (CMCS) hydrogel inclusion complexes.
32614709	6	61	theme	hydrogel	1203:1210	arg1	network					1212:1218	the hydrogel network	1199:1218	the hydrogel network	1199:1218	In vitro simulated drug release test of composite hydrogel showed that the hydrogel network swelled or degraded, the micelles were lysed, and the release of the drug was triggered when the composite hydrogels were exposed to the micro-environment with high pH or high DTT concentration.
32614709	2	62	contain	containing	437:446	arg1	polymer					429:435	a hydrophilic segment PEG-SS-COOH polymer	395:435	a hydrophilic segment PEG-SS-COOH polymer containing a disulfide bond	395:463	Dithiodipropionic acid and poly(ethylene glycol) (PEG) were esterified to synthesize a hydrophilic segment PEG-SS-COOH polymer containing a disulfide bond, and ε-caprolactone is ring-opened in Ar atmosphere to obtain a hydrophobic segment HO-PCL-OH homopolymer.
32614709	2	62	contain	containing	437:446	arg2	bond					460:463	a disulfide bond	448:463	a disulfide bond	448:463	Dithiodipropionic acid and poly(ethylene glycol) (PEG) were esterified to synthesize a hydrophilic segment PEG-SS-COOH polymer containing a disulfide bond, and ε-caprolactone is ring-opened in Ar atmosphere to obtain a hydrophobic segment HO-PCL-OH homopolymer.
32614709	1	63	dep	PEG-SS-PCL-SS-PEG	221:237	arg1	complexes					299:307	hydrogel inclusion complexes	280:307	hydrogel inclusion complexes	280:307	A pH and redox dual-responsive composite hydrogel was developed, which was formed from triblock copolymer PEG-SS-PCL-SS-PEG and Ag/AgO/carboxymethyl chitosan (CMCS) hydrogel inclusion complexes.
32614709	1	64	theme	dual-responsive	130:144	arg1	hydrogel					156:163	redox dual-responsive composite hydrogel	124:163	redox dual-responsive composite hydrogel	124:163	A pH and redox dual-responsive composite hydrogel was developed, which was formed from triblock copolymer PEG-SS-PCL-SS-PEG and Ag/AgO/carboxymethyl chitosan (CMCS) hydrogel inclusion complexes.
32614709	5	65	theme	reducing	1107:1114	arg1	substances					1116:1125	reducing substances	1107:1125	reducing substances	1107:1125	The results showed that the micelles had redox sensitivity due to the insertion of disulfide bonds, and had certain stability within 24 h without reducing substances.
32614709	1	66	theme	Ag/AgO/carboxymethyl	243:262	arg1	chitosan					264:271	Ag/AgO/carboxymethyl chitosan	243:271	Ag/AgO/carboxymethyl chitosan (CMCS)	243:278	A pH and redox dual-responsive composite hydrogel was developed, which was formed from triblock copolymer PEG-SS-PCL-SS-PEG and Ag/AgO/carboxymethyl chitosan (CMCS) hydrogel inclusion complexes.
32614709	1	66	theme	Ag/AgO/carboxymethyl	243:262	arg1	CMCS					274:277	CMCS	274:277	CMCS	274:277	A pH and redox dual-responsive composite hydrogel was developed, which was formed from triblock copolymer PEG-SS-PCL-SS-PEG and Ag/AgO/carboxymethyl chitosan (CMCS) hydrogel inclusion complexes.
32614709	1	67	theme	composite	146:154	arg1	hydrogel					156:163	redox dual-responsive composite hydrogel	124:163	redox dual-responsive composite hydrogel	124:163	A pH and redox dual-responsive composite hydrogel was developed, which was formed from triblock copolymer PEG-SS-PCL-SS-PEG and Ag/AgO/carboxymethyl chitosan (CMCS) hydrogel inclusion complexes.
32614709	5	68	contain	had	1065:1067	arg2	stability					1077:1085	certain stability	1069:1085	certain stability	1069:1085	The results showed that the micelles had redox sensitivity due to the insertion of disulfide bonds, and had certain stability within 24 h without reducing substances.
32614709	5	68	contain	had	1065:1067	arg1	micelles					989:996	the micelles	985:996	the micelles	985:996	The results showed that the micelles had redox sensitivity due to the insertion of disulfide bonds, and had certain stability within 24 h without reducing substances.
32614709	3	69	from	micelles	699:706	arg1	solution					722:729	an aqueous solution	711:729	an aqueous solution	711:729	The PEG-SS-PCL-SS-PEG triblock polymers were prepared by reacting the two in a certain ratio, and spontaneously assembled into micelles in an aqueous solution.
32614709	7	70	theme	delivery	1520:1527	arg1	system					1529:1534	the dual drug delivery system	1506:1534	the dual drug delivery system	1506:1534	Therefore, this composite hydrogel could potentially be one of the candidate materials for the dual drug delivery system.
32614709	6	71	theme	composite	1168:1176	arg1	hydrogel					1178:1185	composite hydrogel	1168:1185	composite hydrogel	1168:1185	In vitro simulated drug release test of composite hydrogel showed that the hydrogel network swelled or degraded, the micelles were lysed, and the release of the drug was triggered when the composite hydrogels were exposed to the micro-environment with high pH or high DTT concentration.
32614709	2	72	theme	segment	541:547	arg1	homopolymer					559:569	a hydrophobic segment HO-PCL-OH homopolymer	527:569	a hydrophobic segment HO-PCL-OH homopolymer	527:569	Dithiodipropionic acid and poly(ethylene glycol) (PEG) were esterified to synthesize a hydrophilic segment PEG-SS-COOH polymer containing a disulfide bond, and ε-caprolactone is ring-opened in Ar atmosphere to obtain a hydrophobic segment HO-PCL-OH homopolymer.
33231600	10	0	theme	gut	1231:1233	arg1	microbiota					1235:1244	gut microbiota	1231:1244	gut microbiota	1231:1244	Thus, β-glucan showed a similar probiotic activity to inulin and is expected to be a potential prebiotic for the modulation of gut microbiota.
33231600	0	1	theme	probiotic	56:64	arg1	activity					66:73	a similar probiotic activity	46:73	a similar probiotic activity to inulin	46:83	Yeast β-glucan, a potential prebiotic, showed a similar probiotic activity to inulin.
33231600	8	2	theme	Bifidobacterium	998:1012	arg1	growth					988:993	the growth	984:993	the growth of Bifidobacterium	984:1012	Furthermore, both β-glucan and inulin could selectively promote the growth of Bifidobacterium.
33231600	6	3	theme	microbiota	752:761	arg1	proliferation					723:735	the proliferation	719:735	the proliferation of harmful gut microbiota	719:761	At the same time, β-glucan could modulate the structure and composition of gut microbiota by inhibiting the proliferation of harmful gut microbiota and promoting the growth of health-promoting gut microbiota.
33231600	6	4	theme	gut	748:750	arg1	microbiota					752:761	harmful gut microbiota	740:761	harmful gut microbiota	740:761	At the same time, β-glucan could modulate the structure and composition of gut microbiota by inhibiting the proliferation of harmful gut microbiota and promoting the growth of health-promoting gut microbiota.
33231600	1	5	contain	has	131:133	arg2	activities					160:169	various health-promoting activities	135:169	various health-promoting activities	135:169	β-Glucan, an economical by-product of yeast, has various health-promoting activities.
33231600	1	5	contain	has	131:133	arg1	by-product					110:119	an economical by-product	96:119	an economical by-product of yeast	96:128	β-Glucan, an economical by-product of yeast, has various health-promoting activities.
33231600	1	5	contain	has	131:133	arg1	β-Glucan					86:93	β-Glucan	86:93	β-Glucan	86:93	β-Glucan, an economical by-product of yeast, has various health-promoting activities.
33231600	4	6	dep	showed	423:428	arg1	hydrolyzed					452:461	hydrolyzed	452:461	showed β-glucan could not be hydrolyzed in saliva, gastric and small intestinal conditions	423:512	The results showed β-glucan could not be hydrolyzed in saliva, gastric and small intestinal conditions.
33231600	7	7	theme	phylum	831:836	arg1	level					838:842	the phylum level	827:842	the phylum level	827:842	At the phylum level, β-glucan significantly decreased the ratio of Firmicutes to Bacteroidetes.
33231600	1	8	theme	various	135:141	arg1	activities					160:169	various health-promoting activities	135:169	various health-promoting activities	135:169	β-Glucan, an economical by-product of yeast, has various health-promoting activities.
33231600	3	9	theme	intestinal	328:337	arg1	solutions					339:347	gastric and small intestinal solutions	310:347	gastric and small intestinal solutions	310:347	Thus, simulated digestion under saliva, gastric and small intestinal solutions and fermentation by gut microbiota were studied in this work.
33231600	5	10	theme	large	542:546	arg1	intestine					548:556	the large intestine	538:556	the large intestine	538:556	Then, β-glucan reaches the large intestine, where it is degraded and metabolized by gut microbiota.
33231600	1	11	theme	health-promoting	143:158	arg1	activities					160:169	various health-promoting activities	135:169	various health-promoting activities	135:169	β-Glucan, an economical by-product of yeast, has various health-promoting activities.
33231600	3	12	theme	gut	369:371	arg1	microbiota					373:382	gut microbiota	369:382	gut microbiota	369:382	Thus, simulated digestion under saliva, gastric and small intestinal solutions and fermentation by gut microbiota were studied in this work.
33231600	5	13	theme	gut	599:601	arg1	microbiota					603:612	gut microbiota	599:612	gut microbiota	599:612	Then, β-glucan reaches the large intestine, where it is degraded and metabolized by gut microbiota.
33231600	0	14	theme	similar	48:54	arg1	activity					66:73	a similar probiotic activity	46:73	a similar probiotic activity to inulin	46:83	Yeast β-glucan, a potential prebiotic, showed a similar probiotic activity to inulin.
33231600	0	15	theme	Yeast	0:4	arg1	prebiotic					28:36	a potential prebiotic	16:36	a potential prebiotic	16:36	Yeast β-glucan, a potential prebiotic, showed a similar probiotic activity to inulin.
33231600	0	15	theme	Yeast	0:4	arg1	β-glucan					6:13	Yeast β-glucan	0:13	Yeast β-glucan	0:13	Yeast β-glucan, a potential prebiotic, showed a similar probiotic activity to inulin.
33231600	6	16	theme	harmful	740:746	arg1	microbiota					752:761	harmful gut microbiota	740:761	harmful gut microbiota	740:761	At the same time, β-glucan could modulate the structure and composition of gut microbiota by inhibiting the proliferation of harmful gut microbiota and promoting the growth of health-promoting gut microbiota.
33231600	6	17	theme	microbiota	812:821	arg1	growth					781:786	the growth	777:786	the growth of health-promoting gut microbiota	777:821	At the same time, β-glucan could modulate the structure and composition of gut microbiota by inhibiting the proliferation of harmful gut microbiota and promoting the growth of health-promoting gut microbiota.
33231600	7	18	theme	Firmicutes	891:900	arg1	ratio					882:886	the ratio	878:886	the ratio of Firmicutes to Bacteroidetes	878:917	At the phylum level, β-glucan significantly decreased the ratio of Firmicutes to Bacteroidetes.
33231600	6	19	dep	structure	661:669	arg1	the					657:659	the	657:659	the	657:659	At the same time, β-glucan could modulate the structure and composition of gut microbiota by inhibiting the proliferation of harmful gut microbiota and promoting the growth of health-promoting gut microbiota.
33231600	9	20	theme	Bifidobacterium_longum	1080:1101	arg1	growth					1070:1075	the growth	1066:1075	the growth of Bifidobacterium_longum	1066:1101	Unlike inulin, β-glucan was able to better promote the growth of Bifidobacterium_longum.
33231600	2	21	from	yeast	245:249	arg1	characteristics					212:226	the digestion and fermentation characteristics	181:226	characteristics	212:226	However, the digestion and fermentation characteristics of β-glucan from yeast are still unknown.
33231600	2	21	from	yeast	245:249	arg1	unknown					261:267	unknown	261:267	unknown	261:267	However, the digestion and fermentation characteristics of β-glucan from yeast are still unknown.
33231600	0	22	theme	potential	18:26	arg1	prebiotic					28:36	a potential prebiotic	16:36	a potential prebiotic	16:36	Yeast β-glucan, a potential prebiotic, showed a similar probiotic activity to inulin.
33231600	0	22	theme	potential	18:26	arg1	β-glucan					6:13	Yeast β-glucan	0:13	Yeast β-glucan	0:13	Yeast β-glucan, a potential prebiotic, showed a similar probiotic activity to inulin.
33231600	4	23	theme	intestinal	492:501	arg1	saliva					466:471	saliva	466:471	saliva	466:471	The results showed β-glucan could not be hydrolyzed in saliva, gastric and small intestinal conditions.
33231600	4	23	theme	intestinal	492:501	arg1	conditions					503:512	gastric and small intestinal conditions	474:512	gastric and small intestinal conditions	474:512	The results showed β-glucan could not be hydrolyzed in saliva, gastric and small intestinal conditions.
33231600	2	24	theme	fermentation	199:210	arg1	characteristics					212:226	the digestion and fermentation characteristics	181:226	characteristics	212:226	However, the digestion and fermentation characteristics of β-glucan from yeast are still unknown.
33231600	2	24	theme	fermentation	199:210	arg1	unknown					261:267	unknown	261:267	unknown	261:267	However, the digestion and fermentation characteristics of β-glucan from yeast are still unknown.
33231600	10	25	theme	similar	1128:1134	arg1	activity					1146:1153	a similar probiotic activity	1126:1153	a similar probiotic activity	1126:1153	Thus, β-glucan showed a similar probiotic activity to inulin and is expected to be a potential prebiotic for the modulation of gut microbiota.
33231600	2	26	theme	digestion	185:193	arg1	characteristics					212:226	the digestion and fermentation characteristics	181:226	characteristics	212:226	However, the digestion and fermentation characteristics of β-glucan from yeast are still unknown.
33231600	2	26	theme	digestion	185:193	arg1	unknown					261:267	unknown	261:267	unknown	261:267	However, the digestion and fermentation characteristics of β-glucan from yeast are still unknown.
33231600	6	27	theme	same	622:625	arg1	time					627:630	the same time	618:630	the same time	618:630	At the same time, β-glucan could modulate the structure and composition of gut microbiota by inhibiting the proliferation of harmful gut microbiota and promoting the growth of health-promoting gut microbiota.
33231600	6	28	theme	gut	808:810	arg1	microbiota					812:821	health-promoting gut microbiota	791:821	health-promoting gut microbiota	791:821	At the same time, β-glucan could modulate the structure and composition of gut microbiota by inhibiting the proliferation of harmful gut microbiota and promoting the growth of health-promoting gut microbiota.
33231600	1	29	theme	economical	99:108	arg1	by-product					110:119	an economical by-product	96:119	an economical by-product of yeast	96:128	β-Glucan, an economical by-product of yeast, has various health-promoting activities.
33231600	1	29	theme	economical	99:108	arg1	β-Glucan					86:93	β-Glucan	86:93	β-Glucan	86:93	β-Glucan, an economical by-product of yeast, has various health-promoting activities.
33231600	3	30	theme	simulated	276:284	arg1	digestion					286:294	simulated digestion	276:294	simulated digestion under saliva, gastric and small intestinal solutions and fermentation by gut microbiota	276:382	Thus, simulated digestion under saliva, gastric and small intestinal solutions and fermentation by gut microbiota were studied in this work.
33231600	6	31	theme	health-promoting	791:806	arg1	microbiota					812:821	health-promoting gut microbiota	791:821	health-promoting gut microbiota	791:821	At the same time, β-glucan could modulate the structure and composition of gut microbiota by inhibiting the proliferation of harmful gut microbiota and promoting the growth of health-promoting gut microbiota.
33231600	6	32	theme	microbiota	694:703	arg1	composition					675:685	composition	675:685	composition	675:685	At the same time, β-glucan could modulate the structure and composition of gut microbiota by inhibiting the proliferation of harmful gut microbiota and promoting the growth of health-promoting gut microbiota.
33231600	6	32	theme	microbiota	694:703	arg1	structure					661:669	structure	661:669	structure	661:669	At the same time, β-glucan could modulate the structure and composition of gut microbiota by inhibiting the proliferation of harmful gut microbiota and promoting the growth of health-promoting gut microbiota.
33231600	3	33	theme	gastric	310:316	arg1	solutions					339:347	gastric and small intestinal solutions	310:347	gastric and small intestinal solutions	310:347	Thus, simulated digestion under saliva, gastric and small intestinal solutions and fermentation by gut microbiota were studied in this work.
33231600	6	34	theme	gut	690:692	arg1	microbiota					694:703	gut microbiota	690:703	gut microbiota	690:703	At the same time, β-glucan could modulate the structure and composition of gut microbiota by inhibiting the proliferation of harmful gut microbiota and promoting the growth of health-promoting gut microbiota.
33231600	10	35	theme	potential	1189:1197	arg1	β-glucan					1110:1117	β-glucan	1110:1117	β-glucan	1110:1117	Thus, β-glucan showed a similar probiotic activity to inulin and is expected to be a potential prebiotic for the modulation of gut microbiota.
33231600	10	35	theme	potential	1189:1197	arg1	prebiotic					1199:1207	a potential prebiotic	1187:1207	a potential prebiotic for the modulation of gut microbiota	1187:1244	Thus, β-glucan showed a similar probiotic activity to inulin and is expected to be a potential prebiotic for the modulation of gut microbiota.
33231600	4	36	theme	gastric	474:480	arg1	saliva					466:471	saliva	466:471	saliva	466:471	The results showed β-glucan could not be hydrolyzed in saliva, gastric and small intestinal conditions.
33231600	4	36	theme	gastric	474:480	arg1	conditions					503:512	gastric and small intestinal conditions	474:512	gastric and small intestinal conditions	474:512	The results showed β-glucan could not be hydrolyzed in saliva, gastric and small intestinal conditions.
33231600	2	37	theme	β-glucan	231:238	arg1	characteristics					212:226	the digestion and fermentation characteristics	181:226	characteristics	212:226	However, the digestion and fermentation characteristics of β-glucan from yeast are still unknown.
33231600	2	37	theme	β-glucan	231:238	arg1	unknown					261:267	unknown	261:267	unknown	261:267	However, the digestion and fermentation characteristics of β-glucan from yeast are still unknown.
33231600	1	38	theme	yeast	124:128	arg1	by-product					110:119	an economical by-product	96:119	an economical by-product of yeast	96:128	β-Glucan, an economical by-product of yeast, has various health-promoting activities.
33231600	1	38	theme	yeast	124:128	arg1	β-Glucan					86:93	β-Glucan	86:93	β-Glucan	86:93	β-Glucan, an economical by-product of yeast, has various health-promoting activities.
33231600	10	39	theme	microbiota	1235:1244	arg1	modulation					1217:1226	the modulation	1213:1226	the modulation of gut microbiota	1213:1244	Thus, β-glucan showed a similar probiotic activity to inulin and is expected to be a potential prebiotic for the modulation of gut microbiota.
33231600	10	40	theme	probiotic	1136:1144	arg1	activity					1146:1153	a similar probiotic activity	1126:1153	a similar probiotic activity	1126:1153	Thus, β-glucan showed a similar probiotic activity to inulin and is expected to be a potential prebiotic for the modulation of gut microbiota.
33248696	2	0	theme	diverse	489:495	arg1	properties					497:506	diverse properties	489:506	diverse properties	489:506	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	8	1	theme	combination	1485:1495	arg1	use					1470:1472	the use	1466:1472	the use of a novel combination of all-natural, sustainable nanoscale building blocks	1466:1549	Our work opens up new scenarios on the use of a novel combination of all-natural, sustainable nanoscale building blocks to develop aqueous "superfoams" which are highly stable, stimulable and processable.
33248696	4	2	theme	small	721:725	arg1	shear					751:755	small deformation oscillatory shear	721:755	small deformation oscillatory shear	721:755	A range of microscopy techniques and small deformation oscillatory shear were adopted to examine the microstructure and viscoelasticity of foams, and a stabilization mechanism for highly stable foams was then established.
33248696	0	3	theme	acid	88:91	arg1	nanofibrils					93:103	glycyrrhizic acid nanofibrils	75:103	glycyrrhizic acid nanofibrils	75:103	Highly stable and thermo-responsive gel foams by synergistically combining glycyrrhizic acid nanofibrils and cellulose nanocrystals.
33248696	7	4	theme	rapid	1324:1328	arg1	destabilization					1340:1354	a rapid on-demand destabilization	1322:1354	a rapid on-demand destabilization upon heating	1322:1367	Such ultra-stable gel foams display tunable thermo-responsive behavior and a rapid on-demand destabilization upon heating by inducing a phase transition of the bulk composite network.
33248696	2	5	theme	cellulose	367:375	arg1	nanocrystals					377:388	rigid nanofiller cellulose nanocrystals	350:388	rigid nanofiller cellulose nanocrystals (CNCs)	350:395	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	2	5	theme	cellulose	367:375	arg1	CNCs					391:394	CNCs	391:394	CNCs	391:394	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	1	6	theme	aqueous	257:263	arg1	foams					276:280	aqueous food-grade foams	257:280	aqueous food-grade foams	257:280	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	0	7	theme	cellulose	109:117	arg1	nanocrystals					119:130	cellulose nanocrystals	109:130	cellulose nanocrystals	109:130	Highly stable and thermo-responsive gel foams by synergistically combining glycyrrhizic acid nanofibrils and cellulose nanocrystals.
33248696	4	8	theme	foams	823:827	arg1	viscoelasticity					804:818	viscoelasticity	804:818	viscoelasticity	804:818	A range of microscopy techniques and small deformation oscillatory shear were adopted to examine the microstructure and viscoelasticity of foams, and a stabilization mechanism for highly stable foams was then established.
33248696	4	8	theme	foams	823:827	arg1	microstructure					785:798	microstructure	785:798	microstructure	785:798	A range of microscopy techniques and small deformation oscillatory shear were adopted to examine the microstructure and viscoelasticity of foams, and a stabilization mechanism for highly stable foams was then established.
33248696	4	9	theme	microscopy	695:704	arg1	techniques					706:715	microscopy techniques	695:715	microscopy techniques	695:715	A range of microscopy techniques and small deformation oscillatory shear were adopted to examine the microstructure and viscoelasticity of foams, and a stabilization mechanism for highly stable foams was then established.
33248696	2	10	theme	synergistic	295:305	arg1	combination					307:317	the synergistic combination	291:317	the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs)	291:395	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	8	11	theme	novel	1479:1483	arg1	combination					1485:1495	a novel combination	1477:1495	a novel combination of all-natural, sustainable nanoscale building blocks	1477:1549	Our work opens up new scenarios on the use of a novel combination of all-natural, sustainable nanoscale building blocks to develop aqueous "superfoams" which are highly stable, stimulable and processable.
33248696	1	12	theme	food-grade	265:274	arg1	foams					276:280	aqueous food-grade foams	257:280	aqueous food-grade foams	257:280	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	6	13	theme	responsible	1209:1219	arg1	factor					1202:1207	the key factor	1194:1207	the key factor responsible for their high stability	1194:1244	FINDINGS CNCs are homogeneously distributed in the architecture and mechanically reinforce the GNF fibrillar network, leading to a highly viscoelastic composite network in the continuous phase of foams, which is the key factor responsible for their high stability.
33248696	6	13	theme	responsible	1209:1219	arg1	network					1143:1149	a highly viscoelastic composite network	1111:1149	a highly viscoelastic composite network	1111:1149	FINDINGS CNCs are homogeneously distributed in the architecture and mechanically reinforce the GNF fibrillar network, leading to a highly viscoelastic composite network in the continuous phase of foams, which is the key factor responsible for their high stability.
33248696	6	14	theme	high	1231:1234	arg1	stability					1236:1244	their high stability	1225:1244	their high stability	1225:1244	FINDINGS CNCs are homogeneously distributed in the architecture and mechanically reinforce the GNF fibrillar network, leading to a highly viscoelastic composite network in the continuous phase of foams, which is the key factor responsible for their high stability.
33248696	6	15	theme	continuous	1158:1167	arg1	phase					1169:1173	the continuous phase	1154:1173	the continuous phase of foams	1154:1182	FINDINGS CNCs are homogeneously distributed in the architecture and mechanically reinforce the GNF fibrillar network, leading to a highly viscoelastic composite network in the continuous phase of foams, which is the key factor responsible for their high stability.
33248696	1	16	theme	glycyrrhizic	160:171	arg1	nanofibrils					190:200	GA nanofibrils	187:200	GA nanofibrils (GNFs)	187:207	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	1	16	theme	glycyrrhizic	160:171	arg1	GA					179:180	GA	179:180	GA	179:180	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	1	16	theme	glycyrrhizic	160:171	arg1	agents					231:236	effective foaming agents	213:236	effective foaming agents for formulation of aqueous food-grade foams	213:280	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	1	16	theme	glycyrrhizic	160:171	arg1	acid					173:176	HYPOTHESIS Natural saponin glycyrrhizic acid	133:176	HYPOTHESIS Natural saponin glycyrrhizic acid (GA)	133:181	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	1	17	theme	HYPOTHESIS	133:142	arg1	nanofibrils					190:200	GA nanofibrils	187:200	GA nanofibrils (GNFs)	187:207	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	1	17	theme	HYPOTHESIS	133:142	arg1	GA					179:180	GA	179:180	GA	179:180	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	1	17	theme	HYPOTHESIS	133:142	arg1	agents					231:236	effective foaming agents	213:236	effective foaming agents for formulation of aqueous food-grade foams	213:280	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	1	17	theme	HYPOTHESIS	133:142	arg1	acid					173:176	HYPOTHESIS Natural saponin glycyrrhizic acid	133:176	HYPOTHESIS Natural saponin glycyrrhizic acid (GA)	133:181	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	6	18	from	network	1143:1149	arg1	phase					1169:1173	the continuous phase	1154:1173	the continuous phase of foams	1154:1182	FINDINGS CNCs are homogeneously distributed in the architecture and mechanically reinforce the GNF fibrillar network, leading to a highly viscoelastic composite network in the continuous phase of foams, which is the key factor responsible for their high stability.
33248696	8	19	theme	new	1449:1451	arg1	scenarios					1453:1461	new scenarios	1449:1461	new scenarios on the use of a novel combination of all-natural, sustainable nanoscale building blocks	1449:1549	Our work opens up new scenarios on the use of a novel combination of all-natural, sustainable nanoscale building blocks to develop aqueous "superfoams" which are highly stable, stimulable and processable.
33248696	4	20	theme	stabilization	836:848	arg1	mechanism					850:858	a stabilization mechanism	834:858	a stabilization mechanism for highly stable foams	834:882	A range of microscopy techniques and small deformation oscillatory shear were adopted to examine the microstructure and viscoelasticity of foams, and a stabilization mechanism for highly stable foams was then established.
33248696	1	21	theme	foams	276:280	arg1	formulation					242:252	formulation	242:252	formulation of aqueous food-grade foams	242:280	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	4	22	theme	stable	871:876	arg1	foams					878:882	highly stable foams	864:882	highly stable foams	864:882	A range of microscopy techniques and small deformation oscillatory shear were adopted to examine the microstructure and viscoelasticity of foams, and a stabilization mechanism for highly stable foams was then established.
33248696	6	23	theme	key	1198:1200	arg1	factor					1202:1207	the key factor	1194:1207	the key factor responsible for their high stability	1194:1244	FINDINGS CNCs are homogeneously distributed in the architecture and mechanically reinforce the GNF fibrillar network, leading to a highly viscoelastic composite network in the continuous phase of foams, which is the key factor responsible for their high stability.
33248696	6	23	theme	key	1198:1200	arg1	network					1143:1149	a highly viscoelastic composite network	1111:1149	a highly viscoelastic composite network	1111:1149	FINDINGS CNCs are homogeneously distributed in the architecture and mechanically reinforce the GNF fibrillar network, leading to a highly viscoelastic composite network in the continuous phase of foams, which is the key factor responsible for their high stability.
33248696	1	24	theme	GA	187:188	arg1	nanofibrils					190:200	GA nanofibrils	187:200	GA nanofibrils (GNFs)	187:207	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	1	24	theme	GA	187:188	arg1	acid					173:176	HYPOTHESIS Natural saponin glycyrrhizic acid	133:176	HYPOTHESIS Natural saponin glycyrrhizic acid (GA)	133:181	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	1	24	theme	GA	187:188	arg1	agents					231:236	effective foaming agents	213:236	effective foaming agents for formulation of aqueous food-grade foams	213:280	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	1	24	theme	GA	187:188	arg1	GNFs					203:206	GNFs	203:206	GNFs	203:206	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	0	25	theme	stable	7:12	arg1	foams					40:44	Highly stable and thermo-responsive gel foams	0:44	Highly stable and thermo-responsive gel foams	0:44	Highly stable and thermo-responsive gel foams by synergistically combining glycyrrhizic acid nanofibrils and cellulose nanocrystals.
33248696	7	26	theme	tunable	1283:1289	arg1	behavior					1309:1316	tunable thermo-responsive behavior	1283:1316	tunable thermo-responsive behavior	1283:1316	Such ultra-stable gel foams display tunable thermo-responsive behavior and a rapid on-demand destabilization upon heating by inducing a phase transition of the bulk composite network.
33248696	2	27	theme	semiflexible	327:338	arg1	GNFs					340:343	soft semiflexible GNFs	322:343	soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs)	322:395	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	8	28	from	scenarios	1453:1461	arg1	use					1470:1472	the use	1466:1472	the use of a novel combination of all-natural, sustainable nanoscale building blocks	1466:1549	Our work opens up new scenarios on the use of a novel combination of all-natural, sustainable nanoscale building blocks to develop aqueous "superfoams" which are highly stable, stimulable and processable.
33248696	6	29	theme	composite	1133:1141	arg1	factor					1202:1207	the key factor	1194:1207	the key factor responsible for their high stability	1194:1244	FINDINGS CNCs are homogeneously distributed in the architecture and mechanically reinforce the GNF fibrillar network, leading to a highly viscoelastic composite network in the continuous phase of foams, which is the key factor responsible for their high stability.
33248696	6	29	theme	composite	1133:1141	arg1	network					1143:1149	a highly viscoelastic composite network	1111:1149	a highly viscoelastic composite network	1111:1149	FINDINGS CNCs are homogeneously distributed in the architecture and mechanically reinforce the GNF fibrillar network, leading to a highly viscoelastic composite network in the continuous phase of foams, which is the key factor responsible for their high stability.
33248696	8	30	theme	superfoams	1571:1580	arg1	"					1581:1581	aqueous "superfoams"	1562:1581	aqueous "superfoams" which are highly stable, stimulable and processable	1562:1633	Our work opens up new scenarios on the use of a novel combination of all-natural, sustainable nanoscale building blocks to develop aqueous "superfoams" which are highly stable, stimulable and processable.
33248696	4	31	theme	shear	751:755	arg1	range					686:690	A range	684:690	A range of microscopy techniques and small deformation oscillatory shear	684:755	A range of microscopy techniques and small deformation oscillatory shear were adopted to examine the microstructure and viscoelasticity of foams, and a stabilization mechanism for highly stable foams was then established.
33248696	2	32	theme	soft	322:325	arg1	GNFs					340:343	soft semiflexible GNFs	322:343	soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs)	322:395	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	6	33	theme	viscoelastic	1120:1131	arg1	factor					1202:1207	the key factor	1194:1207	the key factor responsible for their high stability	1194:1244	FINDINGS CNCs are homogeneously distributed in the architecture and mechanically reinforce the GNF fibrillar network, leading to a highly viscoelastic composite network in the continuous phase of foams, which is the key factor responsible for their high stability.
33248696	6	33	theme	viscoelastic	1120:1131	arg1	network					1143:1149	a highly viscoelastic composite network	1111:1149	a highly viscoelastic composite network	1111:1149	FINDINGS CNCs are homogeneously distributed in the architecture and mechanically reinforce the GNF fibrillar network, leading to a highly viscoelastic composite network in the continuous phase of foams, which is the key factor responsible for their high stability.
33248696	2	34	theme	composite	439:447	arg1	foams					449:453	advanced composite foams	430:453	advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness	430:558	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	2	34	theme	composite	439:447	arg1	stability					523:531	high stability	518:531	high stability	518:531	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	2	34	theme	composite	439:447	arg1	stimuli					537:543	stimuli	537:543	stimuli	537:543	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	3	35	contain	containing	579:588	arg2	mixtures					590:597	mixtures	590:597	mixtures of GNFs and CNCs	590:614	EXPERIMENTS Foams containing mixtures of GNFs and CNCs were prepared, and their formation and stability were investigated.
33248696	3	35	contain	containing	579:588	arg1	Foams					573:577	EXPERIMENTS Foams	561:577	EXPERIMENTS Foams containing mixtures of GNFs and CNCs	561:614	EXPERIMENTS Foams containing mixtures of GNFs and CNCs were prepared, and their formation and stability were investigated.
33248696	2	36	dep	stability	523:531	arg1	responsiveness					545:558	responsiveness	545:558	responsiveness	545:558	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	7	37	theme	network	1422:1428	arg1	transition					1389:1398	a phase transition	1381:1398	a phase transition of the bulk composite network	1381:1428	Such ultra-stable gel foams display tunable thermo-responsive behavior and a rapid on-demand destabilization upon heating by inducing a phase transition of the bulk composite network.
33248696	6	38	theme	GNF	1077:1079	arg1	network					1091:1097	the GNF fibrillar network	1073:1097	the GNF fibrillar network	1073:1097	FINDINGS CNCs are homogeneously distributed in the architecture and mechanically reinforce the GNF fibrillar network, leading to a highly viscoelastic composite network in the continuous phase of foams, which is the key factor responsible for their high stability.
33248696	2	39	theme	advanced	430:437	arg1	foams					449:453	advanced composite foams	430:453	advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness	430:558	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	2	39	theme	advanced	430:437	arg1	stability					523:531	high stability	518:531	high stability	518:531	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	2	39	theme	advanced	430:437	arg1	stimuli					537:543	stimuli	537:543	stimuli	537:543	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	8	40	theme	blocks	1544:1549	arg1	combination					1485:1495	a novel combination	1477:1495	a novel combination of all-natural, sustainable nanoscale building blocks	1477:1549	Our work opens up new scenarios on the use of a novel combination of all-natural, sustainable nanoscale building blocks to develop aqueous "superfoams" which are highly stable, stimulable and processable.
33248696	4	41	dep	microstructure	785:798	arg1	the					781:783	the	781:783	the	781:783	A range of microscopy techniques and small deformation oscillatory shear were adopted to examine the microstructure and viscoelasticity of foams, and a stabilization mechanism for highly stable foams was then established.
33248696	7	42	theme	phase	1383:1387	arg1	transition					1389:1398	a phase transition	1381:1398	a phase transition of the bulk composite network	1381:1428	Such ultra-stable gel foams display tunable thermo-responsive behavior and a rapid on-demand destabilization upon heating by inducing a phase transition of the bulk composite network.
33248696	2	43	theme	rigid	350:354	arg1	nanocrystals					377:388	rigid nanofiller cellulose nanocrystals	350:388	rigid nanofiller cellulose nanocrystals (CNCs)	350:395	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	2	43	theme	rigid	350:354	arg1	CNCs					391:394	CNCs	391:394	CNCs	391:394	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	2	44	with	GNFs	340:343	arg1	nanocrystals					377:388	rigid nanofiller cellulose nanocrystals	350:388	rigid nanofiller cellulose nanocrystals (CNCs)	350:395	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	2	44	with	GNFs	340:343	arg1	CNCs					391:394	CNCs	391:394	CNCs	391:394	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	0	45	theme	gel	36:38	arg1	foams					40:44	Highly stable and thermo-responsive gel foams	0:44	Highly stable and thermo-responsive gel foams	0:44	Highly stable and thermo-responsive gel foams by synergistically combining glycyrrhizic acid nanofibrils and cellulose nanocrystals.
33248696	2	46	theme	complex	467:473	arg1	structure					475:483	a more complex structure	460:483	a more complex structure	460:483	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	0	47	theme	thermo-responsive	18:34	arg1	foams					40:44	Highly stable and thermo-responsive gel foams	0:44	Highly stable and thermo-responsive gel foams	0:44	Highly stable and thermo-responsive gel foams by synergistically combining glycyrrhizic acid nanofibrils and cellulose nanocrystals.
33248696	8	48	theme	all-natural	1500:1510	arg1	blocks					1544:1549	all-natural, sustainable nanoscale building blocks	1500:1549	all-natural, sustainable nanoscale building blocks	1500:1549	Our work opens up new scenarios on the use of a novel combination of all-natural, sustainable nanoscale building blocks to develop aqueous "superfoams" which are highly stable, stimulable and processable.
33248696	1	49	theme	effective	213:221	arg1	nanofibrils					190:200	GA nanofibrils	187:200	GA nanofibrils (GNFs)	187:207	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	1	49	theme	effective	213:221	arg1	agents					231:236	effective foaming agents	213:236	effective foaming agents for formulation of aqueous food-grade foams	213:280	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	1	49	theme	effective	213:221	arg1	acid					173:176	HYPOTHESIS Natural saponin glycyrrhizic acid	133:176	HYPOTHESIS Natural saponin glycyrrhizic acid (GA)	133:181	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	7	50	theme	ultra-stable	1252:1263	arg1	foams					1269:1273	Such ultra-stable gel foams	1247:1273	Such ultra-stable gel foams	1247:1273	Such ultra-stable gel foams display tunable thermo-responsive behavior and a rapid on-demand destabilization upon heating by inducing a phase transition of the bulk composite network.
33248696	2	51	theme	nanofiller	356:365	arg1	nanocrystals					377:388	rigid nanofiller cellulose nanocrystals	350:388	rigid nanofiller cellulose nanocrystals (CNCs)	350:395	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	2	51	theme	nanofiller	356:365	arg1	CNCs					391:394	CNCs	391:394	CNCs	391:394	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	8	52	dep	all-natural	1500:1510	arg1	sustainable					1513:1523	sustainable	1513:1523	sustainable	1513:1523	Our work opens up new scenarios on the use of a novel combination of all-natural, sustainable nanoscale building blocks to develop aqueous "superfoams" which are highly stable, stimulable and processable.
33248696	7	53	theme	Such	1247:1250	arg1	foams					1269:1273	Such ultra-stable gel foams	1247:1273	Such ultra-stable gel foams	1247:1273	Such ultra-stable gel foams display tunable thermo-responsive behavior and a rapid on-demand destabilization upon heating by inducing a phase transition of the bulk composite network.
33248696	8	54	theme	building	1535:1542	arg1	blocks					1544:1549	all-natural, sustainable nanoscale building blocks	1500:1549	all-natural, sustainable nanoscale building blocks	1500:1549	Our work opens up new scenarios on the use of a novel combination of all-natural, sustainable nanoscale building blocks to develop aqueous "superfoams" which are highly stable, stimulable and processable.
33248696	3	55	theme	CNCs	611:614	arg1	mixtures					590:597	mixtures	590:597	mixtures of GNFs and CNCs	590:614	EXPERIMENTS Foams containing mixtures of GNFs and CNCs were prepared, and their formation and stability were investigated.
33248696	7	56	theme	gel	1265:1267	arg1	foams					1269:1273	Such ultra-stable gel foams	1247:1273	Such ultra-stable gel foams	1247:1273	Such ultra-stable gel foams display tunable thermo-responsive behavior and a rapid on-demand destabilization upon heating by inducing a phase transition of the bulk composite network.
33248696	1	57	theme	Natural	144:150	arg1	nanofibrils					190:200	GA nanofibrils	187:200	GA nanofibrils (GNFs)	187:207	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	1	57	theme	Natural	144:150	arg1	GA					179:180	GA	179:180	GA	179:180	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	1	57	theme	Natural	144:150	arg1	agents					231:236	effective foaming agents	213:236	effective foaming agents for formulation of aqueous food-grade foams	213:280	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	1	57	theme	Natural	144:150	arg1	acid					173:176	HYPOTHESIS Natural saponin glycyrrhizic acid	133:176	HYPOTHESIS Natural saponin glycyrrhizic acid (GA)	133:181	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	6	58	theme	FINDINGS	982:989	arg1	CNCs					991:994	FINDINGS CNCs	982:994	FINDINGS CNCs	982:994	FINDINGS CNCs are homogeneously distributed in the architecture and mechanically reinforce the GNF fibrillar network, leading to a highly viscoelastic composite network in the continuous phase of foams, which is the key factor responsible for their high stability.
33248696	4	59	theme	oscillatory	739:749	arg1	shear					751:755	small deformation oscillatory shear	721:755	small deformation oscillatory shear	721:755	A range of microscopy techniques and small deformation oscillatory shear were adopted to examine the microstructure and viscoelasticity of foams, and a stabilization mechanism for highly stable foams was then established.
33248696	8	60	theme	nanoscale	1525:1533	arg1	blocks					1544:1549	all-natural, sustainable nanoscale building blocks	1500:1549	all-natural, sustainable nanoscale building blocks	1500:1549	Our work opens up new scenarios on the use of a novel combination of all-natural, sustainable nanoscale building blocks to develop aqueous "superfoams" which are highly stable, stimulable and processable.
33248696	2	61	with	foams	449:453	arg1	properties					497:506	diverse properties	489:506	diverse properties	489:506	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	2	61	with	foams	449:453	arg1	structure					475:483	a more complex structure	460:483	a more complex structure	460:483	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	1	62	theme	saponin	152:158	arg1	nanofibrils					190:200	GA nanofibrils	187:200	GA nanofibrils (GNFs)	187:207	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	1	62	theme	saponin	152:158	arg1	GA					179:180	GA	179:180	GA	179:180	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	1	62	theme	saponin	152:158	arg1	agents					231:236	effective foaming agents	213:236	effective foaming agents for formulation of aqueous food-grade foams	213:280	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	1	62	theme	saponin	152:158	arg1	acid					173:176	HYPOTHESIS Natural saponin glycyrrhizic acid	133:176	HYPOTHESIS Natural saponin glycyrrhizic acid (GA)	133:181	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	2	63	theme	high	518:521	arg1	stability					523:531	high stability	518:531	high stability	518:531	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	1	64	theme	foaming	223:229	arg1	nanofibrils					190:200	GA nanofibrils	187:200	GA nanofibrils (GNFs)	187:207	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	1	64	theme	foaming	223:229	arg1	agents					231:236	effective foaming agents	213:236	effective foaming agents for formulation of aqueous food-grade foams	213:280	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	1	64	theme	foaming	223:229	arg1	acid					173:176	HYPOTHESIS Natural saponin glycyrrhizic acid	133:176	HYPOTHESIS Natural saponin glycyrrhizic acid (GA)	133:181	HYPOTHESIS Natural saponin glycyrrhizic acid (GA) and GA nanofibrils (GNFs) are effective foaming agents for formulation of aqueous food-grade foams.
33248696	5	65	theme	foams	961:965	arg1	destabilization					942:956	the temperature-responsive destabilization	915:956	the temperature-responsive destabilization of foams	915:965	Further, the temperature-responsive destabilization of foams was evaluated.
33248696	3	66	theme	EXPERIMENTS	561:571	arg1	Foams					573:577	EXPERIMENTS Foams	561:577	EXPERIMENTS Foams containing mixtures of GNFs and CNCs	561:614	EXPERIMENTS Foams containing mixtures of GNFs and CNCs were prepared, and their formation and stability were investigated.
33248696	2	67	theme	GNFs	340:343	arg1	combination					307:317	the synergistic combination	291:317	the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs)	291:395	Through the synergistic combination of soft semiflexible GNFs with rigid nanofiller cellulose nanocrystals (CNCs), it should be possible to create advanced composite foams with a more complex structure and diverse properties including high stability and stimuli responsiveness.
33248696	7	68	theme	on-demand	1330:1338	arg1	destabilization					1340:1354	a rapid on-demand destabilization	1322:1354	a rapid on-demand destabilization upon heating	1322:1367	Such ultra-stable gel foams display tunable thermo-responsive behavior and a rapid on-demand destabilization upon heating by inducing a phase transition of the bulk composite network.
33248696	0	69	theme	glycyrrhizic	75:86	arg1	nanofibrils					93:103	glycyrrhizic acid nanofibrils	75:103	glycyrrhizic acid nanofibrils	75:103	Highly stable and thermo-responsive gel foams by synergistically combining glycyrrhizic acid nanofibrils and cellulose nanocrystals.
33248696	5	70	theme	temperature-responsive	919:940	arg1	destabilization					942:956	the temperature-responsive destabilization	915:956	the temperature-responsive destabilization of foams	915:965	Further, the temperature-responsive destabilization of foams was evaluated.
33248696	7	71	theme	composite	1412:1420	arg1	network					1422:1428	the bulk composite network	1403:1428	the bulk composite network	1403:1428	Such ultra-stable gel foams display tunable thermo-responsive behavior and a rapid on-demand destabilization upon heating by inducing a phase transition of the bulk composite network.
33248696	4	72	theme	techniques	706:715	arg1	range					686:690	A range	684:690	A range of microscopy techniques and small deformation oscillatory shear	684:755	A range of microscopy techniques and small deformation oscillatory shear were adopted to examine the microstructure and viscoelasticity of foams, and a stabilization mechanism for highly stable foams was then established.
33248696	7	73	theme	bulk	1407:1410	arg1	network					1422:1428	the bulk composite network	1403:1428	the bulk composite network	1403:1428	Such ultra-stable gel foams display tunable thermo-responsive behavior and a rapid on-demand destabilization upon heating by inducing a phase transition of the bulk composite network.
33248696	6	74	theme	fibrillar	1081:1089	arg1	network					1091:1097	the GNF fibrillar network	1073:1097	the GNF fibrillar network	1073:1097	FINDINGS CNCs are homogeneously distributed in the architecture and mechanically reinforce the GNF fibrillar network, leading to a highly viscoelastic composite network in the continuous phase of foams, which is the key factor responsible for their high stability.
33248696	7	75	theme	thermo-responsive	1291:1307	arg1	behavior					1309:1316	tunable thermo-responsive behavior	1283:1316	tunable thermo-responsive behavior	1283:1316	Such ultra-stable gel foams display tunable thermo-responsive behavior and a rapid on-demand destabilization upon heating by inducing a phase transition of the bulk composite network.
33248696	8	76	theme	aqueous	1562:1568	arg1	"					1581:1581	aqueous "superfoams"	1562:1581	aqueous "superfoams" which are highly stable, stimulable and processable	1562:1633	Our work opens up new scenarios on the use of a novel combination of all-natural, sustainable nanoscale building blocks to develop aqueous "superfoams" which are highly stable, stimulable and processable.
33248696	6	77	theme	foams	1178:1182	arg1	phase					1169:1173	the continuous phase	1154:1173	the continuous phase of foams	1154:1182	FINDINGS CNCs are homogeneously distributed in the architecture and mechanically reinforce the GNF fibrillar network, leading to a highly viscoelastic composite network in the continuous phase of foams, which is the key factor responsible for their high stability.
33248696	3	78	theme	GNFs	602:605	arg1	mixtures					590:597	mixtures	590:597	mixtures of GNFs and CNCs	590:614	EXPERIMENTS Foams containing mixtures of GNFs and CNCs were prepared, and their formation and stability were investigated.
33248696	4	79	theme	deformation	727:737	arg1	shear					751:755	small deformation oscillatory shear	721:755	small deformation oscillatory shear	721:755	A range of microscopy techniques and small deformation oscillatory shear were adopted to examine the microstructure and viscoelasticity of foams, and a stabilization mechanism for highly stable foams was then established.
34331513	8	0	theme	bioactive	1632:1640	arg1	signal					1652:1657	bioactive inorganic signal	1632:1657	bioactive inorganic signal	1632:1657	Our results highlight the possibility of achieving smart CS/HA based composites able to promote a great osteogenic differentiation of hMSC by increasing the amount of HA nanoparticles used as bioactive inorganic signal.
34331513	6	1	theme	human	1189:1193	arg1	cells					1212:1216	human mesenchymal stem cells	1189:1216	human mesenchymal stem cells (hMSC)	1189:1223	The results obtained have demonstrated that by increasing inorganic component concentration, CS/HA (60 and 70% v/v) scaffolds induced a good biological response in terms of osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype.
34331513	6	1	theme	human	1189:1193	arg1	hMSC					1219:1222	hMSC	1219:1222	hMSC	1219:1222	The results obtained have demonstrated that by increasing inorganic component concentration, CS/HA (60 and 70% v/v) scaffolds induced a good biological response in terms of osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype.
34331513	6	2	theme	differentiation	1170:1184	arg1	terms					1150:1154	terms	1150:1154	terms of osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype	1150:1252	The results obtained have demonstrated that by increasing inorganic component concentration, CS/HA (60 and 70% v/v) scaffolds induced a good biological response in terms of osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype.
34331513	4	3	theme	approach	644:651	arg1	combination					621:631	The combination	617:631	The combination of sol-gel approach and freeze-drying technology	617:680	The combination of sol-gel approach and freeze-drying technology was used to obtain CS/HA scaffolds with three-dimensional (3D) porous structure suitable for cell in-growth.
34331513	7	4	with	scaffolds	1272:1280	arg1	concentration					1294:1306	higher concentration	1287:1306	higher concentration of inorganic fillers	1287:1327	Furthermore, the scaffolds with higher concentration of inorganic fillers are able to modulate the production of pro-inflammatory (TGF-β) and anti-inflammatory (IL-4, IL-10) cytokines.
34331513	3	5	theme	cell	535:538	arg1	models					540:545	in vitro bone cell models	521:545	in vitro bone cell models for their applications in the field of bone tissue engineering (BTE)	521:614	Herein, the bioactivity of these composite systems was analyzed on in vitro bone cell models for their applications in the field of bone tissue engineering (BTE).
34331513	5	6	theme	bioactive	849:857	arg1	scaffolds					869:877	bioactive composite scaffolds	849:877	bioactive composite scaffolds	849:877	Specifically, our aim was to investigate the influence of bioactive composite scaffolds on cellular behavior in terms of osteoinductivity and anti-inflammatory effects for treating bone defects.
34331513	4	7	theme	freeze-drying	657:669	arg1	technology					671:680	freeze-drying technology	657:680	freeze-drying technology	657:680	The combination of sol-gel approach and freeze-drying technology was used to obtain CS/HA scaffolds with three-dimensional (3D) porous structure suitable for cell in-growth.
34331513	1	8	theme	inorganic	196:204	arg1	signals					216:222	inorganic bioactive signals	196:222	inorganic bioactive signals such as hydroxyapatite (HA)	196:250	Considerable attention has been given to the use of chitosan (CS)-based materials reinforced with inorganic bioactive signals such as hydroxyapatite (HA) to treat bone defects and tissue loss.
34331513	1	8	theme	inorganic	196:204	arg1	hydroxyapatite					232:245	hydroxyapatite	232:245	hydroxyapatite (HA)	232:250	Considerable attention has been given to the use of chitosan (CS)-based materials reinforced with inorganic bioactive signals such as hydroxyapatite (HA) to treat bone defects and tissue loss.
34331513	7	9	theme	anti-inflammatory	1397:1413	arg1	cytokines					1429:1437	pro-inflammatory (TGF-β) and anti-inflammatory (IL-4, IL-10) cytokines	1368:1437	pro-inflammatory (TGF-β) and anti-inflammatory (IL-4, IL-10) cytokines	1368:1437	Furthermore, the scaffolds with higher concentration of inorganic fillers are able to modulate the production of pro-inflammatory (TGF-β) and anti-inflammatory (IL-4, IL-10) cytokines.
34331513	6	10	theme	osteoblast	1233:1242	arg1	phenotype					1244:1252	osteoblast phenotype	1233:1252	osteoblast phenotype	1233:1252	The results obtained have demonstrated that by increasing inorganic component concentration, CS/HA (60 and 70% v/v) scaffolds induced a good biological response in terms of osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype.
34331513	3	11	theme	tissue	591:596	arg1	BTE					611:613	BTE	611:613	BTE	611:613	Herein, the bioactivity of these composite systems was analyzed on in vitro bone cell models for their applications in the field of bone tissue engineering (BTE).
34331513	3	11	theme	tissue	591:596	arg1	engineering					598:608	bone tissue engineering	586:608	bone tissue engineering (BTE)	586:614	Herein, the bioactivity of these composite systems was analyzed on in vitro bone cell models for their applications in the field of bone tissue engineering (BTE).
34331513	7	12	dep	pro-inflammatory	1368:1383	arg1	TGF-β					1386:1390	TGF-β	1386:1390	TGF-β	1386:1390	Furthermore, the scaffolds with higher concentration of inorganic fillers are able to modulate the production of pro-inflammatory (TGF-β) and anti-inflammatory (IL-4, IL-10) cytokines.
34331513	9	13	theme	response	1743:1750	arg1	induction					1711:1719	the induction	1707:1719	the induction of a pro-inflammatory response in bone implant site	1707:1771	Contemporarily, these materials allow avoiding the induction of a pro-inflammatory response in bone implant site.
34331513	4	14	theme	CS/HA	701:705	arg1	scaffolds					707:715	CS/HA scaffolds	701:715	CS/HA scaffolds	701:715	The combination of sol-gel approach and freeze-drying technology was used to obtain CS/HA scaffolds with three-dimensional (3D) porous structure suitable for cell in-growth.
34331513	9	15	theme	bone	1755:1758	arg1	site					1768:1771	bone implant site	1755:1771	bone implant site	1755:1771	Contemporarily, these materials allow avoiding the induction of a pro-inflammatory response in bone implant site.
34331513	3	16	from	applications	557:568	arg1	field					577:581	the field	573:581	the field of bone tissue engineering (BTE)	573:614	Herein, the bioactivity of these composite systems was analyzed on in vitro bone cell models for their applications in the field of bone tissue engineering (BTE).
34331513	7	17	theme	cytokines	1429:1437	arg1	production					1354:1363	the production	1350:1363	the production of pro-inflammatory (TGF-β) and anti-inflammatory (IL-4, IL-10) cytokines	1350:1437	Furthermore, the scaffolds with higher concentration of inorganic fillers are able to modulate the production of pro-inflammatory (TGF-β) and anti-inflammatory (IL-4, IL-10) cytokines.
34331513	1	18	theme	chitosan	150:157	arg1	CS					160:161	CS	160:161	CS	160:161	Considerable attention has been given to the use of chitosan (CS)-based materials reinforced with inorganic bioactive signals such as hydroxyapatite (HA) to treat bone defects and tissue loss.
34331513	1	18	theme	chitosan	150:157	arg1	materials					170:178	chitosan (CS)-based materials	150:178	chitosan (CS)-based materials reinforced with inorganic bioactive signals such as hydroxyapatite (HA)	150:250	Considerable attention has been given to the use of chitosan (CS)-based materials reinforced with inorganic bioactive signals such as hydroxyapatite (HA) to treat bone defects and tissue loss.
34331513	5	19	theme	osteoinductivity	912:927	arg1	terms					903:907	terms	903:907	terms of osteoinductivity and anti-inflammatory effects for treating bone defects	903:983	Specifically, our aim was to investigate the influence of bioactive composite scaffolds on cellular behavior in terms of osteoinductivity and anti-inflammatory effects for treating bone defects.
34331513	6	20	theme	stem	1207:1210	arg1	cells					1212:1216	human mesenchymal stem cells	1189:1216	human mesenchymal stem cells (hMSC)	1189:1223	The results obtained have demonstrated that by increasing inorganic component concentration, CS/HA (60 and 70% v/v) scaffolds induced a good biological response in terms of osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype.
34331513	6	20	theme	stem	1207:1210	arg1	hMSC					1219:1222	hMSC	1219:1222	hMSC	1219:1222	The results obtained have demonstrated that by increasing inorganic component concentration, CS/HA (60 and 70% v/v) scaffolds induced a good biological response in terms of osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype.
34331513	6	21	theme	CS/HA	1079:1083	arg1	scaffolds					1102:1110	CS/HA (60 and 70% v/v) scaffolds	1079:1110	CS/HA (60 and 70% v/v) scaffolds	1079:1110	The results obtained have demonstrated that by increasing inorganic component concentration, CS/HA (60 and 70% v/v) scaffolds induced a good biological response in terms of osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype.
34331513	5	22	theme	cellular	882:889	arg1	behavior					891:898	cellular behavior	882:898	cellular behavior in terms of osteoinductivity and anti-inflammatory effects for treating bone defects	882:983	Specifically, our aim was to investigate the influence of bioactive composite scaffolds on cellular behavior in terms of osteoinductivity and anti-inflammatory effects for treating bone defects.
34331513	8	23	theme	CS/HA	1497:1501	arg1	composites					1509:1518	smart CS/HA based composites	1491:1518	smart CS/HA based composites able to promote a great osteogenic differentiation of hMSC by increasing the amount of HA nanoparticles used as bioactive inorganic signal	1491:1657	Our results highlight the possibility of achieving smart CS/HA based composites able to promote a great osteogenic differentiation of hMSC by increasing the amount of HA nanoparticles used as bioactive inorganic signal.
34331513	5	24	from	behavior	891:898	arg1	terms					903:907	terms	903:907	terms of osteoinductivity and anti-inflammatory effects for treating bone defects	903:983	Specifically, our aim was to investigate the influence of bioactive composite scaffolds on cellular behavior in terms of osteoinductivity and anti-inflammatory effects for treating bone defects.
34331513	1	25	theme	-based	163:168	arg1	CS					160:161	CS	160:161	CS	160:161	Considerable attention has been given to the use of chitosan (CS)-based materials reinforced with inorganic bioactive signals such as hydroxyapatite (HA) to treat bone defects and tissue loss.
34331513	1	25	theme	-based	163:168	arg1	materials					170:178	chitosan (CS)-based materials	150:178	chitosan (CS)-based materials reinforced with inorganic bioactive signals such as hydroxyapatite (HA)	150:250	Considerable attention has been given to the use of chitosan (CS)-based materials reinforced with inorganic bioactive signals such as hydroxyapatite (HA) to treat bone defects and tissue loss.
34331513	6	26	theme	cells	1212:1216	arg1	differentiation					1170:1184	osteogenic differentiation	1159:1184	osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype	1159:1252	The results obtained have demonstrated that by increasing inorganic component concentration, CS/HA (60 and 70% v/v) scaffolds induced a good biological response in terms of osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype.
34331513	2	27	contain	possess	335:341	arg2	reactions					364:372	minimal foreign body reactions	343:372	minimal foreign body reactions	343:372	It is well known that CS/HA based materials possess minimal foreign body reactions, good biocompatibility, controlled biodegradability and antibacterial property.
34331513	2	27	contain	possess	335:341	arg1	materials					325:333	CS/HA based materials	313:333	CS/HA based materials	313:333	It is well known that CS/HA based materials possess minimal foreign body reactions, good biocompatibility, controlled biodegradability and antibacterial property.
34331513	2	27	contain	possess	335:341	arg2	biocompatibility					380:395	good biocompatibility	375:395	good biocompatibility	375:395	It is well known that CS/HA based materials possess minimal foreign body reactions, good biocompatibility, controlled biodegradability and antibacterial property.
34331513	2	27	contain	possess	335:341	arg2	property					444:451	antibacterial property	430:451	antibacterial property	430:451	It is well known that CS/HA based materials possess minimal foreign body reactions, good biocompatibility, controlled biodegradability and antibacterial property.
34331513	2	27	contain	possess	335:341	arg2	biodegradability					409:424	controlled biodegradability	398:424	controlled biodegradability	398:424	It is well known that CS/HA based materials possess minimal foreign body reactions, good biocompatibility, controlled biodegradability and antibacterial property.
34331513	4	28	used	used	686:689	arg2	combination					621:631	The combination	617:631	The combination of sol-gel approach and freeze-drying technology	617:680	The combination of sol-gel approach and freeze-drying technology was used to obtain CS/HA scaffolds with three-dimensional (3D) porous structure suitable for cell in-growth.
34331513	0	29	theme	Chitosan/hydroxyapatite	0:22	arg1	nanocomposite					24:36	Chitosan/hydroxyapatite nanocomposite	0:36	Chitosan/hydroxyapatite nanocomposite	0:36	Chitosan/hydroxyapatite nanocomposite scaffolds to modulate osteogenic and inflammatory response.
34331513	5	30	theme	effects	951:957	arg1	terms					903:907	terms	903:907	terms of osteoinductivity and anti-inflammatory effects for treating bone defects	903:983	Specifically, our aim was to investigate the influence of bioactive composite scaffolds on cellular behavior in terms of osteoinductivity and anti-inflammatory effects for treating bone defects.
34331513	8	31	theme	osteogenic	1544:1553	arg1	differentiation					1555:1569	a great osteogenic differentiation	1536:1569	a great osteogenic differentiation of hMSC	1536:1577	Our results highlight the possibility of achieving smart CS/HA based composites able to promote a great osteogenic differentiation of hMSC by increasing the amount of HA nanoparticles used as bioactive inorganic signal.
34331513	7	32	theme	fillers	1321:1327	arg1	concentration					1294:1306	higher concentration	1287:1306	higher concentration of inorganic fillers	1287:1327	Furthermore, the scaffolds with higher concentration of inorganic fillers are able to modulate the production of pro-inflammatory (TGF-β) and anti-inflammatory (IL-4, IL-10) cytokines.
34331513	2	33	theme	controlled	398:407	arg1	biodegradability					409:424	controlled biodegradability	398:424	controlled biodegradability	398:424	It is well known that CS/HA based materials possess minimal foreign body reactions, good biocompatibility, controlled biodegradability and antibacterial property.
34331513	6	34	theme	component	1054:1062	arg1	concentration					1064:1076	inorganic component concentration	1044:1076	inorganic component concentration	1044:1076	The results obtained have demonstrated that by increasing inorganic component concentration, CS/HA (60 and 70% v/v) scaffolds induced a good biological response in terms of osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype.
34331513	3	35	theme	systems	497:503	arg1	bioactivity					466:476	the bioactivity	462:476	the bioactivity of these composite systems	462:503	Herein, the bioactivity of these composite systems was analyzed on in vitro bone cell models for their applications in the field of bone tissue engineering (BTE).
34331513	6	36	theme	good	1122:1125	arg1	response					1138:1145	a good biological response	1120:1145	a good biological response	1120:1145	The results obtained have demonstrated that by increasing inorganic component concentration, CS/HA (60 and 70% v/v) scaffolds induced a good biological response in terms of osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype.
34331513	2	37	theme	CS/HA	313:317	arg1	materials					325:333	CS/HA based materials	313:333	CS/HA based materials	313:333	It is well known that CS/HA based materials possess minimal foreign body reactions, good biocompatibility, controlled biodegradability and antibacterial property.
34331513	9	38	from	site	1768:1771	arg1	induction					1711:1719	the induction	1707:1719	the induction of a pro-inflammatory response in bone implant site	1707:1771	Contemporarily, these materials allow avoiding the induction of a pro-inflammatory response in bone implant site.
34331513	3	39	theme	in	521:522	arg1	models					540:545	in vitro bone cell models	521:545	in vitro bone cell models for their applications in the field of bone tissue engineering (BTE)	521:614	Herein, the bioactivity of these composite systems was analyzed on in vitro bone cell models for their applications in the field of bone tissue engineering (BTE).
34331513	8	40	theme	nanoparticles	1610:1622	arg1	nanoparticles					1610:1622	HA nanoparticles	1607:1622	HA nanoparticles	1607:1622	Our results highlight the possibility of achieving smart CS/HA based composites able to promote a great osteogenic differentiation of hMSC by increasing the amount of HA nanoparticles used as bioactive inorganic signal.
34331513	8	40	theme	nanoparticles	1610:1622	arg1	amount					1597:1602	the amount	1593:1602	the amount of HA nanoparticles used as bioactive inorganic signal	1593:1657	Our results highlight the possibility of achieving smart CS/HA based composites able to promote a great osteogenic differentiation of hMSC by increasing the amount of HA nanoparticles used as bioactive inorganic signal.
34331513	2	41	theme	minimal	343:349	arg1	reactions					364:372	minimal foreign body reactions	343:372	minimal foreign body reactions	343:372	It is well known that CS/HA based materials possess minimal foreign body reactions, good biocompatibility, controlled biodegradability and antibacterial property.
34331513	6	42	theme	%	1095:1095	arg1	CS/HA					1079:1083	CS/HA	1079:1083	CS/HA (60 and 70% v/v) scaffolds	1079:1110	The results obtained have demonstrated that by increasing inorganic component concentration, CS/HA (60 and 70% v/v) scaffolds induced a good biological response in terms of osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype.
34331513	6	42	theme	%	1095:1095	arg1	v/v					1097:1099	60 and 70% v/v	1086:1099	60 and 70% v/v	1086:1099	The results obtained have demonstrated that by increasing inorganic component concentration, CS/HA (60 and 70% v/v) scaffolds induced a good biological response in terms of osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype.
34331513	1	43	theme	bone	261:264	arg1	defects					266:272	bone defects	261:272	bone defects	261:272	Considerable attention has been given to the use of chitosan (CS)-based materials reinforced with inorganic bioactive signals such as hydroxyapatite (HA) to treat bone defects and tissue loss.
34331513	8	44	theme	inorganic	1642:1650	arg1	signal					1652:1657	bioactive inorganic signal	1632:1657	bioactive inorganic signal	1632:1657	Our results highlight the possibility of achieving smart CS/HA based composites able to promote a great osteogenic differentiation of hMSC by increasing the amount of HA nanoparticles used as bioactive inorganic signal.
34331513	3	45	theme	bone	530:533	arg1	models					540:545	in vitro bone cell models	521:545	in vitro bone cell models for their applications in the field of bone tissue engineering (BTE)	521:614	Herein, the bioactivity of these composite systems was analyzed on in vitro bone cell models for their applications in the field of bone tissue engineering (BTE).
34331513	1	46	theme	materials	170:178	arg1	use					143:145	the use	139:145	the use of chitosan (CS)-based materials reinforced with inorganic bioactive signals such as hydroxyapatite (HA) to treat bone defects and tissue loss	139:288	Considerable attention has been given to the use of chitosan (CS)-based materials reinforced with inorganic bioactive signals such as hydroxyapatite (HA) to treat bone defects and tissue loss.
34331513	6	47	theme	osteogenic	1159:1168	arg1	differentiation					1170:1184	osteogenic differentiation	1159:1184	osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype	1159:1252	The results obtained have demonstrated that by increasing inorganic component concentration, CS/HA (60 and 70% v/v) scaffolds induced a good biological response in terms of osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype.
34331513	4	48	theme	sol-gel	636:642	arg1	approach					644:651	sol-gel approach	636:651	sol-gel approach	636:651	The combination of sol-gel approach and freeze-drying technology was used to obtain CS/HA scaffolds with three-dimensional (3D) porous structure suitable for cell in-growth.
34331513	5	49	theme	composite	859:867	arg1	scaffolds					869:877	bioactive composite scaffolds	849:877	bioactive composite scaffolds	849:877	Specifically, our aim was to investigate the influence of bioactive composite scaffolds on cellular behavior in terms of osteoinductivity and anti-inflammatory effects for treating bone defects.
34331513	1	50	theme	bioactive	206:214	arg1	signals					216:222	inorganic bioactive signals	196:222	inorganic bioactive signals such as hydroxyapatite (HA)	196:250	Considerable attention has been given to the use of chitosan (CS)-based materials reinforced with inorganic bioactive signals such as hydroxyapatite (HA) to treat bone defects and tissue loss.
34331513	1	50	theme	bioactive	206:214	arg1	hydroxyapatite					232:245	hydroxyapatite	232:245	hydroxyapatite (HA)	232:250	Considerable attention has been given to the use of chitosan (CS)-based materials reinforced with inorganic bioactive signals such as hydroxyapatite (HA) to treat bone defects and tissue loss.
34331513	7	51	theme	higher	1287:1292	arg1	concentration					1294:1306	higher concentration	1287:1306	higher concentration of inorganic fillers	1287:1327	Furthermore, the scaffolds with higher concentration of inorganic fillers are able to modulate the production of pro-inflammatory (TGF-β) and anti-inflammatory (IL-4, IL-10) cytokines.
34331513	3	52	theme	bone	586:589	arg1	BTE					611:613	BTE	611:613	BTE	611:613	Herein, the bioactivity of these composite systems was analyzed on in vitro bone cell models for their applications in the field of bone tissue engineering (BTE).
34331513	3	52	theme	bone	586:589	arg1	engineering					598:608	bone tissue engineering	586:608	bone tissue engineering (BTE)	586:614	Herein, the bioactivity of these composite systems was analyzed on in vitro bone cell models for their applications in the field of bone tissue engineering (BTE).
34331513	5	53	theme	scaffolds	869:877	arg1	influence					836:844	the influence	832:844	the influence of bioactive composite scaffolds on cellular behavior in terms of osteoinductivity and anti-inflammatory effects for treating bone defects	832:983	Specifically, our aim was to investigate the influence of bioactive composite scaffolds on cellular behavior in terms of osteoinductivity and anti-inflammatory effects for treating bone defects.
34331513	9	54	theme	pro-inflammatory	1726:1741	arg1	response					1743:1750	a pro-inflammatory response	1724:1750	a pro-inflammatory response in bone implant site	1724:1771	Contemporarily, these materials allow avoiding the induction of a pro-inflammatory response in bone implant site.
34331513	5	55	from	influence	836:844	arg1	behavior					891:898	cellular behavior	882:898	cellular behavior in terms of osteoinductivity and anti-inflammatory effects for treating bone defects	882:983	Specifically, our aim was to investigate the influence of bioactive composite scaffolds on cellular behavior in terms of osteoinductivity and anti-inflammatory effects for treating bone defects.
34331513	7	56	dep	cytokines	1429:1437	arg1	IL-4					1416:1419	IL-4	1416:1419	IL-4	1416:1419	Furthermore, the scaffolds with higher concentration of inorganic fillers are able to modulate the production of pro-inflammatory (TGF-β) and anti-inflammatory (IL-4, IL-10) cytokines.
34331513	7	56	dep	cytokines	1429:1437	arg1	IL-10					1422:1426	IL-10	1422:1426	IL-10	1422:1426	Furthermore, the scaffolds with higher concentration of inorganic fillers are able to modulate the production of pro-inflammatory (TGF-β) and anti-inflammatory (IL-4, IL-10) cytokines.
34331513	6	57	theme	inorganic	1044:1052	arg1	concentration					1064:1076	inorganic component concentration	1044:1076	inorganic component concentration	1044:1076	The results obtained have demonstrated that by increasing inorganic component concentration, CS/HA (60 and 70% v/v) scaffolds induced a good biological response in terms of osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype.
34331513	9	58	theme	implant	1760:1766	arg1	site					1768:1771	bone implant site	1755:1771	bone implant site	1755:1771	Contemporarily, these materials allow avoiding the induction of a pro-inflammatory response in bone implant site.
34331513	8	59	theme	smart	1491:1495	arg1	composites					1509:1518	smart CS/HA based composites	1491:1518	smart CS/HA based composites able to promote a great osteogenic differentiation of hMSC by increasing the amount of HA nanoparticles used as bioactive inorganic signal	1491:1657	Our results highlight the possibility of achieving smart CS/HA based composites able to promote a great osteogenic differentiation of hMSC by increasing the amount of HA nanoparticles used as bioactive inorganic signal.
34331513	7	60	theme	pro-inflammatory	1368:1383	arg1	cytokines					1429:1437	pro-inflammatory (TGF-β) and anti-inflammatory (IL-4, IL-10) cytokines	1368:1437	pro-inflammatory (TGF-β) and anti-inflammatory (IL-4, IL-10) cytokines	1368:1437	Furthermore, the scaffolds with higher concentration of inorganic fillers are able to modulate the production of pro-inflammatory (TGF-β) and anti-inflammatory (IL-4, IL-10) cytokines.
34331513	3	61	theme	engineering	598:608	arg1	field					577:581	the field	573:581	the field of bone tissue engineering (BTE)	573:614	Herein, the bioactivity of these composite systems was analyzed on in vitro bone cell models for their applications in the field of bone tissue engineering (BTE).
34331513	9	62	from	response	1743:1750	arg1	site					1768:1771	bone implant site	1755:1771	bone implant site	1755:1771	Contemporarily, these materials allow avoiding the induction of a pro-inflammatory response in bone implant site.
34331513	8	63	theme	based	1503:1507	arg1	composites					1509:1518	smart CS/HA based composites	1491:1518	smart CS/HA based composites able to promote a great osteogenic differentiation of hMSC by increasing the amount of HA nanoparticles used as bioactive inorganic signal	1491:1657	Our results highlight the possibility of achieving smart CS/HA based composites able to promote a great osteogenic differentiation of hMSC by increasing the amount of HA nanoparticles used as bioactive inorganic signal.
34331513	6	64	theme	mesenchymal	1195:1205	arg1	cells					1212:1216	human mesenchymal stem cells	1189:1216	human mesenchymal stem cells (hMSC)	1189:1223	The results obtained have demonstrated that by increasing inorganic component concentration, CS/HA (60 and 70% v/v) scaffolds induced a good biological response in terms of osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype.
34331513	6	64	theme	mesenchymal	1195:1205	arg1	hMSC					1219:1222	hMSC	1219:1222	hMSC	1219:1222	The results obtained have demonstrated that by increasing inorganic component concentration, CS/HA (60 and 70% v/v) scaffolds induced a good biological response in terms of osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype.
34331513	4	65	theme	technology	671:680	arg1	combination					621:631	The combination	617:631	The combination of sol-gel approach and freeze-drying technology	617:680	The combination of sol-gel approach and freeze-drying technology was used to obtain CS/HA scaffolds with three-dimensional (3D) porous structure suitable for cell in-growth.
34331513	8	66	theme	able	1520:1523	arg1	composites					1509:1518	smart CS/HA based composites	1491:1518	smart CS/HA based composites able to promote a great osteogenic differentiation of hMSC by increasing the amount of HA nanoparticles used as bioactive inorganic signal	1491:1657	Our results highlight the possibility of achieving smart CS/HA based composites able to promote a great osteogenic differentiation of hMSC by increasing the amount of HA nanoparticles used as bioactive inorganic signal.
34331513	5	67	theme	bone	972:975	arg1	defects					977:983	bone defects	972:983	bone defects	972:983	Specifically, our aim was to investigate the influence of bioactive composite scaffolds on cellular behavior in terms of osteoinductivity and anti-inflammatory effects for treating bone defects.
34331513	9	68	from	induction	1711:1719	arg1	site					1768:1771	bone implant site	1755:1771	bone implant site	1755:1771	Contemporarily, these materials allow avoiding the induction of a pro-inflammatory response in bone implant site.
34331513	4	69	theme	porous	745:750	arg1	3D					741:742	3D	741:742	3D	741:742	The combination of sol-gel approach and freeze-drying technology was used to obtain CS/HA scaffolds with three-dimensional (3D) porous structure suitable for cell in-growth.
34331513	4	69	theme	porous	745:750	arg1	structure					752:760	three-dimensional (3D) porous structure	722:760	three-dimensional (3D) porous structure suitable for cell in-growth	722:788	The combination of sol-gel approach and freeze-drying technology was used to obtain CS/HA scaffolds with three-dimensional (3D) porous structure suitable for cell in-growth.
34331513	1	70	theme	Considerable	98:109	arg1	attention					111:119	Considerable attention	98:119	Considerable attention	98:119	Considerable attention has been given to the use of chitosan (CS)-based materials reinforced with inorganic bioactive signals such as hydroxyapatite (HA) to treat bone defects and tissue loss.
34331513	8	71	theme	great	1538:1542	arg1	differentiation					1555:1569	a great osteogenic differentiation	1536:1569	a great osteogenic differentiation of hMSC	1536:1577	Our results highlight the possibility of achieving smart CS/HA based composites able to promote a great osteogenic differentiation of hMSC by increasing the amount of HA nanoparticles used as bioactive inorganic signal.
34331513	4	72	theme	suitable	762:769	arg1	3D					741:742	3D	741:742	3D	741:742	The combination of sol-gel approach and freeze-drying technology was used to obtain CS/HA scaffolds with three-dimensional (3D) porous structure suitable for cell in-growth.
34331513	4	72	theme	suitable	762:769	arg1	structure					752:760	three-dimensional (3D) porous structure	722:760	three-dimensional (3D) porous structure suitable for cell in-growth	722:788	The combination of sol-gel approach and freeze-drying technology was used to obtain CS/HA scaffolds with three-dimensional (3D) porous structure suitable for cell in-growth.
34331513	2	73	theme	good	375:378	arg1	biocompatibility					380:395	good biocompatibility	375:395	good biocompatibility	375:395	It is well known that CS/HA based materials possess minimal foreign body reactions, good biocompatibility, controlled biodegradability and antibacterial property.
34331513	5	74	theme	anti-inflammatory	933:949	arg1	effects					951:957	anti-inflammatory effects	933:957	anti-inflammatory effects	933:957	Specifically, our aim was to investigate the influence of bioactive composite scaffolds on cellular behavior in terms of osteoinductivity and anti-inflammatory effects for treating bone defects.
34331513	4	75	theme	three-dimensional	722:738	arg1	3D					741:742	3D	741:742	3D	741:742	The combination of sol-gel approach and freeze-drying technology was used to obtain CS/HA scaffolds with three-dimensional (3D) porous structure suitable for cell in-growth.
34331513	4	75	theme	three-dimensional	722:738	arg1	structure					752:760	three-dimensional (3D) porous structure	722:760	three-dimensional (3D) porous structure suitable for cell in-growth	722:788	The combination of sol-gel approach and freeze-drying technology was used to obtain CS/HA scaffolds with three-dimensional (3D) porous structure suitable for cell in-growth.
34331513	1	76	theme	tissue	278:283	arg1	loss					285:288	tissue loss	278:288	tissue loss	278:288	Considerable attention has been given to the use of chitosan (CS)-based materials reinforced with inorganic bioactive signals such as hydroxyapatite (HA) to treat bone defects and tissue loss.
34331513	3	77	dep	in	521:522	arg1	vitro					524:528	vitro	524:528	vitro	524:528	Herein, the bioactivity of these composite systems was analyzed on in vitro bone cell models for their applications in the field of bone tissue engineering (BTE).
34331513	7	78	theme	inorganic	1311:1319	arg1	fillers					1321:1327	inorganic fillers	1311:1327	inorganic fillers	1311:1327	Furthermore, the scaffolds with higher concentration of inorganic fillers are able to modulate the production of pro-inflammatory (TGF-β) and anti-inflammatory (IL-4, IL-10) cytokines.
34331513	2	79	theme	antibacterial	430:442	arg1	property					444:451	antibacterial property	430:451	antibacterial property	430:451	It is well known that CS/HA based materials possess minimal foreign body reactions, good biocompatibility, controlled biodegradability and antibacterial property.
34331513	8	80	theme	hMSC	1574:1577	arg1	differentiation					1555:1569	a great osteogenic differentiation	1536:1569	a great osteogenic differentiation of hMSC	1536:1577	Our results highlight the possibility of achieving smart CS/HA based composites able to promote a great osteogenic differentiation of hMSC by increasing the amount of HA nanoparticles used as bioactive inorganic signal.
34331513	2	81	theme	body	359:362	arg1	reactions					364:372	minimal foreign body reactions	343:372	minimal foreign body reactions	343:372	It is well known that CS/HA based materials possess minimal foreign body reactions, good biocompatibility, controlled biodegradability and antibacterial property.
34331513	0	82	theme	osteogenic	60:69	arg1	response					88:95	osteogenic and inflammatory response	60:95	osteogenic and inflammatory response	60:95	Chitosan/hydroxyapatite nanocomposite scaffolds to modulate osteogenic and inflammatory response.
34331513	0	83	theme	inflammatory	75:86	arg1	response					88:95	osteogenic and inflammatory response	60:95	osteogenic and inflammatory response	60:95	Chitosan/hydroxyapatite nanocomposite scaffolds to modulate osteogenic and inflammatory response.
34331513	6	84	theme	biological	1127:1136	arg1	response					1138:1145	a good biological response	1120:1145	a good biological response	1120:1145	The results obtained have demonstrated that by increasing inorganic component concentration, CS/HA (60 and 70% v/v) scaffolds induced a good biological response in terms of osteogenic differentiation of human mesenchymal stem cells (hMSC) towards osteoblast phenotype.
34331513	2	85	theme	based	319:323	arg1	materials					325:333	CS/HA based materials	313:333	CS/HA based materials	313:333	It is well known that CS/HA based materials possess minimal foreign body reactions, good biocompatibility, controlled biodegradability and antibacterial property.
34331513	8	86	theme	HA	1607:1608	arg1	nanoparticles					1610:1622	HA nanoparticles	1607:1622	HA nanoparticles	1607:1622	Our results highlight the possibility of achieving smart CS/HA based composites able to promote a great osteogenic differentiation of hMSC by increasing the amount of HA nanoparticles used as bioactive inorganic signal.
34331513	4	87	theme	cell	775:778	arg1	in-growth					780:788	cell in-growth	775:788	cell in-growth	775:788	The combination of sol-gel approach and freeze-drying technology was used to obtain CS/HA scaffolds with three-dimensional (3D) porous structure suitable for cell in-growth.
34331513	3	88	theme	composite	487:495	arg1	systems					497:503	these composite systems	481:503	these composite systems	481:503	Herein, the bioactivity of these composite systems was analyzed on in vitro bone cell models for their applications in the field of bone tissue engineering (BTE).
34331513	2	89	theme	foreign	351:357	arg1	reactions					364:372	minimal foreign body reactions	343:372	minimal foreign body reactions	343:372	It is well known that CS/HA based materials possess minimal foreign body reactions, good biocompatibility, controlled biodegradability and antibacterial property.
34740689	2	0	theme	basic	456:460	arg1	structure					471:479	basic chemical structure	456:479	basic chemical structure	456:479	Cellulose molecules were successfully cross-linked by boron ester bonds, and the original crystal type and basic chemical structure were not changed.
34740689	7	1	theme	tensile	959:965	arg1	strength					967:974	tensile strength	959:974	tensile strength of the composite film with 6 wt% BNC	959:1011	Compared with pure CS film, tensile strength of the composite film with 6 wt% BNC increased about 4.0 times, and its water-vapor permeability decreased about 37%.
34740689	4	2	theme	CS	651:652	arg1	films					654:658	CS films	651:658	CS films	651:658	Addition of NC and BNC to CS films significantly improved their tensile strength and water resistance.
34740689	8	3	theme	novel	1098:1102	arg1	strategy					1104:1111	The novel strategy	1094:1111	The novel strategy for preparing BNC by using boron ester bonds	1094:1156	The novel strategy for preparing BNC by using boron ester bonds will provide a potential approach for the development of starch films with desirable properties.
34740689	7	4	theme	CS	950:951	arg1	film					953:956	pure CS film	945:956	pure CS film	945:956	Compared with pure CS film, tensile strength of the composite film with 6 wt% BNC increased about 4.0 times, and its water-vapor permeability decreased about 37%.
34740689	6	5	theme	resistance	882:891	arg1	properties					893:902	higher mechanical and water resistance properties	854:902	properties	893:902	CS/BNC films showed higher mechanical and water resistance properties compared with CS/NC films.
34740689	6	6	theme	water	876:880	arg1	properties					893:902	higher mechanical and water resistance properties	854:902	properties	893:902	CS/BNC films showed higher mechanical and water resistance properties compared with CS/NC films.
34740689	8	7	theme	desirable	1233:1241	arg1	properties					1243:1252	desirable properties	1233:1252	desirable properties	1233:1252	The novel strategy for preparing BNC by using boron ester bonds will provide a potential approach for the development of starch films with desirable properties.
34740689	8	8	with	films	1222:1226	arg1	properties					1243:1252	desirable properties	1233:1252	desirable properties	1233:1252	The novel strategy for preparing BNC by using boron ester bonds will provide a potential approach for the development of starch films with desirable properties.
34740689	7	9	theme	water-vapor	1048:1058	arg1	permeability					1060:1071	its water-vapor permeability	1044:1071	its water-vapor permeability	1044:1071	Compared with pure CS film, tensile strength of the composite film with 6 wt% BNC increased about 4.0 times, and its water-vapor permeability decreased about 37%.
34740689	7	10	theme	pure	945:948	arg1	film					953:956	pure CS film	945:956	pure CS film	945:956	Compared with pure CS film, tensile strength of the composite film with 6 wt% BNC increased about 4.0 times, and its water-vapor permeability decreased about 37%.
34740689	2	11	theme	Cellulose	349:357	arg1	molecules					359:367	Cellulose molecules	349:367	Cellulose molecules	349:367	Cellulose molecules were successfully cross-linked by boron ester bonds, and the original crystal type and basic chemical structure were not changed.
34740689	5	12	theme	nanocellulose	746:758	arg1	dispersion					732:741	The dispersion	728:741	The dispersion of nanocellulose in CS films	728:770	The dispersion of nanocellulose in CS films was effectively improved by borax cross-linking modification.
34740689	0	13	theme	high	11:14	arg1	films					37:41	Cationized high amylose maize starch films	0:41	Cationized high amylose maize starch films	0:41	Cationized high amylose maize starch films reinforced with borax cross-linked nanocellulose.
34740689	1	14	theme	borax	161:165	arg1	cross-linking					167:179	borax cross-linking	161:179	borax cross-linking	161:179	In this study, a novel strategy for modifying nanocellulose (NC) by borax cross-linking was developed, and the obtained borax modified nanocellulose (BNC) was incorporated into cationized high amylose maize starch (CS) films to evaluate the applicability.
34740689	0	15	theme	Cationized	0:9	arg1	films					37:41	Cationized high amylose maize starch films	0:41	Cationized high amylose maize starch films	0:41	Cationized high amylose maize starch films reinforced with borax cross-linked nanocellulose.
34740689	4	16	theme	tensile	689:695	arg1	strength					697:704	their tensile strength	683:704	their tensile strength	683:704	Addition of NC and BNC to CS films significantly improved their tensile strength and water resistance.
34740689	0	17	theme	maize	24:28	arg1	films					37:41	Cationized high amylose maize starch films	0:41	Cationized high amylose maize starch films	0:41	Cationized high amylose maize starch films reinforced with borax cross-linked nanocellulose.
34740689	4	18	theme	water	710:714	arg1	resistance					716:725	water resistance	710:725	water resistance	710:725	Addition of NC and BNC to CS films significantly improved their tensile strength and water resistance.
34740689	2	19	theme	boron	403:407	arg1	bonds					415:419	boron ester bonds	403:419	boron ester bonds	403:419	Cellulose molecules were successfully cross-linked by boron ester bonds, and the original crystal type and basic chemical structure were not changed.
34740689	2	20	theme	ester	409:413	arg1	bonds					415:419	boron ester bonds	403:419	boron ester bonds	403:419	Cellulose molecules were successfully cross-linked by boron ester bonds, and the original crystal type and basic chemical structure were not changed.
34740689	3	21	theme	relative	521:528	arg1	crystallinity					530:542	the relative crystallinity	517:542	the relative crystallinity of BNC	517:549	Compared with NC, the relative crystallinity of BNC was slightly increased, and the thermal stability was obviously enhanced.
34740689	5	22	theme	borax	800:804	arg1	modification					820:831	borax cross-linking modification	800:831	borax cross-linking modification	800:831	The dispersion of nanocellulose in CS films was effectively improved by borax cross-linking modification.
34740689	1	23	theme	cationized	270:279	arg1	films					312:316	cationized high amylose maize starch (CS) films	270:316	cationized high amylose maize starch (CS) films	270:316	In this study, a novel strategy for modifying nanocellulose (NC) by borax cross-linking was developed, and the obtained borax modified nanocellulose (BNC) was incorporated into cationized high amylose maize starch (CS) films to evaluate the applicability.
34740689	4	24	theme	NC	637:638	arg1	Addition					625:632	Addition	625:632	Addition of NC and BNC to CS films	625:658	Addition of NC and BNC to CS films significantly improved their tensile strength and water resistance.
34740689	5	25	theme	cross-linking	806:818	arg1	modification					820:831	borax cross-linking modification	800:831	borax cross-linking modification	800:831	The dispersion of nanocellulose in CS films was effectively improved by borax cross-linking modification.
34740689	0	26	theme	starch	30:35	arg1	films					37:41	Cationized high amylose maize starch films	0:41	Cationized high amylose maize starch films	0:41	Cationized high amylose maize starch films reinforced with borax cross-linked nanocellulose.
34740689	1	27	theme	high	281:284	arg1	CS					308:309	CS	308:309	CS	308:309	In this study, a novel strategy for modifying nanocellulose (NC) by borax cross-linking was developed, and the obtained borax modified nanocellulose (BNC) was incorporated into cationized high amylose maize starch (CS) films to evaluate the applicability.
34740689	1	27	theme	high	281:284	arg1	starch					300:305	high amylose maize starch	281:305	cationized high amylose maize starch (CS) films	270:316	In this study, a novel strategy for modifying nanocellulose (NC) by borax cross-linking was developed, and the obtained borax modified nanocellulose (BNC) was incorporated into cationized high amylose maize starch (CS) films to evaluate the applicability.
34740689	2	28	theme	crystal	439:445	arg1	type					447:450	the original crystal type	426:450	the original crystal type	426:450	Cellulose molecules were successfully cross-linked by boron ester bonds, and the original crystal type and basic chemical structure were not changed.
34740689	6	29	theme	CS/BNC	834:839	arg1	films					841:845	CS/BNC films	834:845	CS/BNC films	834:845	CS/BNC films showed higher mechanical and water resistance properties compared with CS/NC films.
34740689	7	30	with	strength	967:974	arg1	BNC					1009:1011	6 wt% BNC	1003:1011	6 wt% BNC	1003:1011	Compared with pure CS film, tensile strength of the composite film with 6 wt% BNC increased about 4.0 times, and its water-vapor permeability decreased about 37%.
34740689	7	31	theme	film	993:996	arg1	strength					967:974	tensile strength	959:974	tensile strength of the composite film with 6 wt% BNC	959:1011	Compared with pure CS film, tensile strength of the composite film with 6 wt% BNC increased about 4.0 times, and its water-vapor permeability decreased about 37%.
34740689	6	32	theme	CS/NC	918:922	arg1	films					924:928	CS/NC films	918:928	CS/NC films	918:928	CS/BNC films showed higher mechanical and water resistance properties compared with CS/NC films.
34740689	2	33	theme	original	430:437	arg1	type					447:450	the original crystal type	426:450	the original crystal type	426:450	Cellulose molecules were successfully cross-linked by boron ester bonds, and the original crystal type and basic chemical structure were not changed.
34740689	1	34	theme	amylose	286:292	arg1	CS					308:309	CS	308:309	CS	308:309	In this study, a novel strategy for modifying nanocellulose (NC) by borax cross-linking was developed, and the obtained borax modified nanocellulose (BNC) was incorporated into cationized high amylose maize starch (CS) films to evaluate the applicability.
34740689	1	34	theme	amylose	286:292	arg1	starch					300:305	high amylose maize starch	281:305	cationized high amylose maize starch (CS) films	270:316	In this study, a novel strategy for modifying nanocellulose (NC) by borax cross-linking was developed, and the obtained borax modified nanocellulose (BNC) was incorporated into cationized high amylose maize starch (CS) films to evaluate the applicability.
34740689	2	35	theme	chemical	462:469	arg1	structure					471:479	basic chemical structure	456:479	basic chemical structure	456:479	Cellulose molecules were successfully cross-linked by boron ester bonds, and the original crystal type and basic chemical structure were not changed.
34740689	3	36	theme	BNC	547:549	arg1	crystallinity					530:542	the relative crystallinity	517:542	the relative crystallinity of BNC	517:549	Compared with NC, the relative crystallinity of BNC was slightly increased, and the thermal stability was obviously enhanced.
34740689	5	37	theme	CS	763:764	arg1	films					766:770	CS films	763:770	CS films	763:770	The dispersion of nanocellulose in CS films was effectively improved by borax cross-linking modification.
34740689	1	38	theme	maize	294:298	arg1	CS					308:309	CS	308:309	CS	308:309	In this study, a novel strategy for modifying nanocellulose (NC) by borax cross-linking was developed, and the obtained borax modified nanocellulose (BNC) was incorporated into cationized high amylose maize starch (CS) films to evaluate the applicability.
34740689	1	38	theme	maize	294:298	arg1	starch					300:305	high amylose maize starch	281:305	cationized high amylose maize starch (CS) films	270:316	In this study, a novel strategy for modifying nanocellulose (NC) by borax cross-linking was developed, and the obtained borax modified nanocellulose (BNC) was incorporated into cationized high amylose maize starch (CS) films to evaluate the applicability.
34740689	0	39	link	cross-linked	65:76	arg1	nanocellulose					78:90	borax cross-linked nanocellulose	59:90	borax cross-linked nanocellulose	59:90	Cationized high amylose maize starch films reinforced with borax cross-linked nanocellulose.
34740689	1	40	theme	novel	110:114	arg1	strategy					116:123	a novel strategy	108:123	a novel strategy for modifying nanocellulose (NC) by borax cross-linking	108:179	In this study, a novel strategy for modifying nanocellulose (NC) by borax cross-linking was developed, and the obtained borax modified nanocellulose (BNC) was incorporated into cationized high amylose maize starch (CS) films to evaluate the applicability.
34740689	1	41	theme	starch	300:305	arg1	films					312:316	cationized high amylose maize starch (CS) films	270:316	cationized high amylose maize starch (CS) films	270:316	In this study, a novel strategy for modifying nanocellulose (NC) by borax cross-linking was developed, and the obtained borax modified nanocellulose (BNC) was incorporated into cationized high amylose maize starch (CS) films to evaluate the applicability.
34740689	7	42	theme	6 wt	1003:1006	arg1	BNC					1009:1011	6 wt% BNC	1003:1011	6 wt% BNC	1003:1011	Compared with pure CS film, tensile strength of the composite film with 6 wt% BNC increased about 4.0 times, and its water-vapor permeability decreased about 37%.
34740689	5	43	from	dispersion	732:741	arg1	films					766:770	CS films	763:770	CS films	763:770	The dispersion of nanocellulose in CS films was effectively improved by borax cross-linking modification.
34740689	7	44	theme	composite	983:991	arg1	film					993:996	the composite film	979:996	the composite film	979:996	Compared with pure CS film, tensile strength of the composite film with 6 wt% BNC increased about 4.0 times, and its water-vapor permeability decreased about 37%.
34740689	8	45	theme	ester	1146:1150	arg1	bonds					1152:1156	boron ester bonds	1140:1156	boron ester bonds	1140:1156	The novel strategy for preparing BNC by using boron ester bonds will provide a potential approach for the development of starch films with desirable properties.
34740689	6	46	dep	mechanical	861:870	arg1	higher					854:859	higher	854:859	higher	854:859	CS/BNC films showed higher mechanical and water resistance properties compared with CS/NC films.
34740689	7	47	theme	%	1007:1007	arg1	BNC					1009:1011	6 wt% BNC	1003:1011	6 wt% BNC	1003:1011	Compared with pure CS film, tensile strength of the composite film with 6 wt% BNC increased about 4.0 times, and its water-vapor permeability decreased about 37%.
34740689	3	48	theme	thermal	583:589	arg1	stability					591:599	the thermal stability	579:599	the thermal stability	579:599	Compared with NC, the relative crystallinity of BNC was slightly increased, and the thermal stability was obviously enhanced.
34740689	8	49	theme	potential	1173:1181	arg1	approach					1183:1190	a potential approach	1171:1190	a potential approach for the development of starch films with desirable properties	1171:1252	The novel strategy for preparing BNC by using boron ester bonds will provide a potential approach for the development of starch films with desirable properties.
34740689	1	50	theme	obtained	204:211	arg1	BNC					243:245	BNC	243:245	BNC	243:245	In this study, a novel strategy for modifying nanocellulose (NC) by borax cross-linking was developed, and the obtained borax modified nanocellulose (BNC) was incorporated into cationized high amylose maize starch (CS) films to evaluate the applicability.
34740689	1	50	theme	obtained	204:211	arg1	nanocellulose					228:240	borax modified nanocellulose	213:240	the obtained borax modified nanocellulose (BNC)	200:246	In this study, a novel strategy for modifying nanocellulose (NC) by borax cross-linking was developed, and the obtained borax modified nanocellulose (BNC) was incorporated into cationized high amylose maize starch (CS) films to evaluate the applicability.
34740689	0	51	theme	cross-linked	65:76	arg1	nanocellulose					78:90	borax cross-linked nanocellulose	59:90	borax cross-linked nanocellulose	59:90	Cationized high amylose maize starch films reinforced with borax cross-linked nanocellulose.
34740689	8	52	theme	films	1222:1226	arg1	development					1200:1210	the development	1196:1210	the development of starch films with desirable properties	1196:1252	The novel strategy for preparing BNC by using boron ester bonds will provide a potential approach for the development of starch films with desirable properties.
34740689	8	53	theme	starch	1215:1220	arg1	films					1222:1226	starch films	1215:1226	starch films with desirable properties	1215:1252	The novel strategy for preparing BNC by using boron ester bonds will provide a potential approach for the development of starch films with desirable properties.
34740689	4	54	theme	BNC	644:646	arg1	Addition					625:632	Addition	625:632	Addition of NC and BNC to CS films	625:658	Addition of NC and BNC to CS films significantly improved their tensile strength and water resistance.
34740689	1	55	theme	borax	213:217	arg1	BNC					243:245	BNC	243:245	BNC	243:245	In this study, a novel strategy for modifying nanocellulose (NC) by borax cross-linking was developed, and the obtained borax modified nanocellulose (BNC) was incorporated into cationized high amylose maize starch (CS) films to evaluate the applicability.
34740689	1	55	theme	borax	213:217	arg1	nanocellulose					228:240	borax modified nanocellulose	213:240	the obtained borax modified nanocellulose (BNC)	200:246	In this study, a novel strategy for modifying nanocellulose (NC) by borax cross-linking was developed, and the obtained borax modified nanocellulose (BNC) was incorporated into cationized high amylose maize starch (CS) films to evaluate the applicability.
34740689	0	56	theme	borax	59:63	arg1	nanocellulose					78:90	borax cross-linked nanocellulose	59:90	borax cross-linked nanocellulose	59:90	Cationized high amylose maize starch films reinforced with borax cross-linked nanocellulose.
34740689	0	57	theme	amylose	16:22	arg1	films					37:41	Cationized high amylose maize starch films	0:41	Cationized high amylose maize starch films	0:41	Cationized high amylose maize starch films reinforced with borax cross-linked nanocellulose.
34740689	8	58	theme	boron	1140:1144	arg1	bonds					1152:1156	boron ester bonds	1140:1156	boron ester bonds	1140:1156	The novel strategy for preparing BNC by using boron ester bonds will provide a potential approach for the development of starch films with desirable properties.
34740689	1	59	theme	modified	219:226	arg1	BNC					243:245	BNC	243:245	BNC	243:245	In this study, a novel strategy for modifying nanocellulose (NC) by borax cross-linking was developed, and the obtained borax modified nanocellulose (BNC) was incorporated into cationized high amylose maize starch (CS) films to evaluate the applicability.
34740689	1	59	theme	modified	219:226	arg1	nanocellulose					228:240	borax modified nanocellulose	213:240	the obtained borax modified nanocellulose (BNC)	200:246	In this study, a novel strategy for modifying nanocellulose (NC) by borax cross-linking was developed, and the obtained borax modified nanocellulose (BNC) was incorporated into cationized high amylose maize starch (CS) films to evaluate the applicability.
33984610	0	0	theme	cinnamon	91:98	arg1	oil					110:112	cinnamon essential oil	91:112	cinnamon essential oil	91:112	Fabrication of bioactive binary composite film based on gelatin/chitosan incorporated with cinnamon essential oil and rutin.
33984610	4	1	theme	food	706:709	arg1	solutions					720:728	various food simulant solutions	698:728	various food simulant solutions	698:728	The rutin release from the composite film was evaluated using various food simulant solutions and found that rutin was released faster in acidic and alcoholic solutions.
33984610	7	2	theme	rutin-added	1017:1027	arg1	films					1052:1056	rutin-added chitosan/gelatin-based films	1017:1056	rutin-added chitosan/gelatin-based films	1017:1056	The CEO and rutin-added chitosan/gelatin-based films with improved physical and functional properties can be used for active food packaging applications.
33984610	6	3	theme	rutin	945:949	arg1	use					930:932	the combined use	917:932	the combined use of CEO and rutin	917:949	In addition, the combined use of CEO and rutin showed a synergistic effect of functional properties.
33984610	3	4	theme	vapor	522:526	arg1	properties					536:545	mechanical, thermal, and water vapor barrier properties	491:545	mechanical, thermal, and water vapor barrier properties of the film	491:557	However, the functional material did not affect the physical properties such as mechanical, thermal, and water vapor barrier properties of the film but exhibited high light transmittance with enhanced UV blocking properties.
33984610	3	5	theme	film	554:557	arg1	properties					536:545	mechanical, thermal, and water vapor barrier properties	491:545	mechanical, thermal, and water vapor barrier properties of the film	491:557	However, the functional material did not affect the physical properties such as mechanical, thermal, and water vapor barrier properties of the film but exhibited high light transmittance with enhanced UV blocking properties.
33984610	0	6	theme	essential	100:108	arg1	oil					110:112	cinnamon essential oil	91:112	cinnamon essential oil	91:112	Fabrication of bioactive binary composite film based on gelatin/chitosan incorporated with cinnamon essential oil and rutin.
33984610	6	7	theme	CEO	937:939	arg1	use					930:932	the combined use	917:932	the combined use of CEO and rutin	917:949	In addition, the combined use of CEO and rutin showed a synergistic effect of functional properties.
33984610	4	8	theme	acidic	774:779	arg1	solutions					795:803	acidic and alcoholic solutions	774:803	solutions	795:803	The rutin release from the composite film was evaluated using various food simulant solutions and found that rutin was released faster in acidic and alcoholic solutions.
33984610	4	9	theme	simulant	711:718	arg1	solutions					720:728	various food simulant solutions	698:728	various food simulant solutions	698:728	The rutin release from the composite film was evaluated using various food simulant solutions and found that rutin was released faster in acidic and alcoholic solutions.
33984610	5	10	theme	antioxidant	880:890	arg1	activities					892:901	potent antimicrobial and antioxidant activities	855:901	potent antimicrobial and antioxidant activities	855:901	The chitosan/gelatin-based composite film showed potent antimicrobial and antioxidant activities.
33984610	3	11	theme	mechanical	491:500	arg1	properties					536:545	mechanical, thermal, and water vapor barrier properties	491:545	mechanical, thermal, and water vapor barrier properties of the film	491:557	However, the functional material did not affect the physical properties such as mechanical, thermal, and water vapor barrier properties of the film but exhibited high light transmittance with enhanced UV blocking properties.
33984610	1	12	theme	cinnamon	198:205	arg1	oil					217:219	cinnamon essential oil	198:219	cinnamon essential oil (CEO)	198:225	Chitosan/gelatin-based functional films were fabricated by incorporating cinnamon essential oil (CEO) and rutin for active packaging application.
33984610	1	12	theme	cinnamon	198:205	arg1	CEO					222:224	CEO	222:224	CEO	222:224	Chitosan/gelatin-based functional films were fabricated by incorporating cinnamon essential oil (CEO) and rutin for active packaging application.
33984610	5	13	theme	composite	833:841	arg1	film					843:846	The chitosan/gelatin-based composite film	806:846	The chitosan/gelatin-based composite film	806:846	The chitosan/gelatin-based composite film showed potent antimicrobial and antioxidant activities.
33984610	4	14	theme	alcoholic	785:793	arg1	solutions					795:803	acidic and alcoholic solutions	774:803	solutions	795:803	The rutin release from the composite film was evaluated using various food simulant solutions and found that rutin was released faster in acidic and alcoholic solutions.
33984610	1	15	theme	essential	207:215	arg1	oil					217:219	cinnamon essential oil	198:219	cinnamon essential oil (CEO)	198:225	Chitosan/gelatin-based functional films were fabricated by incorporating cinnamon essential oil (CEO) and rutin for active packaging application.
33984610	1	15	theme	essential	207:215	arg1	CEO					222:224	CEO	222:224	CEO	222:224	Chitosan/gelatin-based functional films were fabricated by incorporating cinnamon essential oil (CEO) and rutin for active packaging application.
33984610	6	16	theme	combined	921:928	arg1	use					930:932	the combined use	917:932	the combined use of CEO and rutin	917:949	In addition, the combined use of CEO and rutin showed a synergistic effect of functional properties.
33984610	7	17	theme	packaging	1135:1143	arg1	applications					1145:1156	active food packaging applications	1123:1156	active food packaging applications	1123:1156	The CEO and rutin-added chitosan/gelatin-based films with improved physical and functional properties can be used for active food packaging applications.
33984610	3	18	theme	light	578:582	arg1	transmittance					584:596	high light transmittance	573:596	high light transmittance with enhanced UV blocking properties	573:633	However, the functional material did not affect the physical properties such as mechanical, thermal, and water vapor barrier properties of the film but exhibited high light transmittance with enhanced UV blocking properties.
33984610	4	19	from	film	673:676	arg1	release					646:652	The rutin release	636:652	The rutin release from the composite film	636:676	The rutin release from the composite film was evaluated using various food simulant solutions and found that rutin was released faster in acidic and alcoholic solutions.
33984610	7	20	used	used	1114:1117	arg2	CEO					1009:1011	CEO	1009:1011	CEO	1009:1011	The CEO and rutin-added chitosan/gelatin-based films with improved physical and functional properties can be used for active food packaging applications.
33984610	7	20	used	used	1114:1117	arg2	films					1052:1056	rutin-added chitosan/gelatin-based films	1017:1056	rutin-added chitosan/gelatin-based films	1017:1056	The CEO and rutin-added chitosan/gelatin-based films with improved physical and functional properties can be used for active food packaging applications.
33984610	7	21	with	CEO	1009:1011	arg1	properties					1096:1105	improved physical and functional properties	1063:1105	improved physical and functional properties	1063:1105	The CEO and rutin-added chitosan/gelatin-based films with improved physical and functional properties can be used for active food packaging applications.
33984610	0	22	theme	binary	25:30	arg1	film					42:45	bioactive binary composite film	15:45	bioactive binary composite film based on gelatin/chitosan	15:71	Fabrication of bioactive binary composite film based on gelatin/chitosan incorporated with cinnamon essential oil and rutin.
33984610	7	23	theme	active	1123:1128	arg1	applications					1145:1156	active food packaging applications	1123:1156	active food packaging applications	1123:1156	The CEO and rutin-added chitosan/gelatin-based films with improved physical and functional properties can be used for active food packaging applications.
33984610	2	24	theme	polymer	325:331	arg1	matrix					333:338	the polymer matrix	321:338	the polymer matrix	321:338	The functional fillers were evenly distributed in the polymer matrix and formed a film compatible with the chitosan/gelatin polymer matrix.
33984610	0	25	theme	bioactive	15:23	arg1	film					42:45	bioactive binary composite film	15:45	bioactive binary composite film based on gelatin/chitosan	15:71	Fabrication of bioactive binary composite film based on gelatin/chitosan incorporated with cinnamon essential oil and rutin.
33984610	7	26	theme	food	1130:1133	arg1	applications					1145:1156	active food packaging applications	1123:1156	active food packaging applications	1123:1156	The CEO and rutin-added chitosan/gelatin-based films with improved physical and functional properties can be used for active food packaging applications.
33984610	3	27	theme	high	573:576	arg1	transmittance					584:596	high light transmittance	573:596	high light transmittance with enhanced UV blocking properties	573:633	However, the functional material did not affect the physical properties such as mechanical, thermal, and water vapor barrier properties of the film but exhibited high light transmittance with enhanced UV blocking properties.
33984610	2	28	theme	polymer	395:401	arg1	matrix					403:408	the chitosan/gelatin polymer matrix	374:408	the chitosan/gelatin polymer matrix	374:408	The functional fillers were evenly distributed in the polymer matrix and formed a film compatible with the chitosan/gelatin polymer matrix.
33984610	0	29	theme	film	42:45	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of bioactive binary composite film based on gelatin/chitosan	0:71	Fabrication of bioactive binary composite film based on gelatin/chitosan incorporated with cinnamon essential oil and rutin.
33984610	6	30	theme	properties	993:1002	arg1	effect					972:977	a synergistic effect	958:977	a synergistic effect of functional properties	958:1002	In addition, the combined use of CEO and rutin showed a synergistic effect of functional properties.
33984610	3	31	theme	UV	612:613	arg1	properties					624:633	enhanced UV blocking properties	603:633	enhanced UV blocking properties	603:633	However, the functional material did not affect the physical properties such as mechanical, thermal, and water vapor barrier properties of the film but exhibited high light transmittance with enhanced UV blocking properties.
33984610	7	32	dep	CEO	1009:1011	arg1	The					1005:1007	The	1005:1007	The	1005:1007	The CEO and rutin-added chitosan/gelatin-based films with improved physical and functional properties can be used for active food packaging applications.
33984610	4	33	theme	composite	663:671	arg1	film					673:676	the composite film	659:676	the composite film	659:676	The rutin release from the composite film was evaluated using various food simulant solutions and found that rutin was released faster in acidic and alcoholic solutions.
33984610	0	34	theme	composite	32:40	arg1	film					42:45	bioactive binary composite film	15:45	bioactive binary composite film based on gelatin/chitosan	15:71	Fabrication of bioactive binary composite film based on gelatin/chitosan incorporated with cinnamon essential oil and rutin.
33984610	6	35	theme	functional	982:991	arg1	properties					993:1002	functional properties	982:1002	functional properties	982:1002	In addition, the combined use of CEO and rutin showed a synergistic effect of functional properties.
33984610	2	36	with	compatible	358:367	arg1	matrix					403:408	the chitosan/gelatin polymer matrix	374:408	the chitosan/gelatin polymer matrix	374:408	The functional fillers were evenly distributed in the polymer matrix and formed a film compatible with the chitosan/gelatin polymer matrix.
33984610	3	37	theme	blocking	615:622	arg1	properties					624:633	enhanced UV blocking properties	603:633	enhanced UV blocking properties	603:633	However, the functional material did not affect the physical properties such as mechanical, thermal, and water vapor barrier properties of the film but exhibited high light transmittance with enhanced UV blocking properties.
33984610	7	38	theme	physical	1072:1079	arg1	properties					1096:1105	improved physical and functional properties	1063:1105	improved physical and functional properties	1063:1105	The CEO and rutin-added chitosan/gelatin-based films with improved physical and functional properties can be used for active food packaging applications.
33984610	5	39	theme	potent	855:860	arg1	activities					892:901	potent antimicrobial and antioxidant activities	855:901	potent antimicrobial and antioxidant activities	855:901	The chitosan/gelatin-based composite film showed potent antimicrobial and antioxidant activities.
33984610	5	40	theme	chitosan/gelatin-based	810:831	arg1	film					843:846	The chitosan/gelatin-based composite film	806:846	The chitosan/gelatin-based composite film	806:846	The chitosan/gelatin-based composite film showed potent antimicrobial and antioxidant activities.
33984610	5	41	theme	antimicrobial	862:874	arg1	activities					892:901	potent antimicrobial and antioxidant activities	855:901	potent antimicrobial and antioxidant activities	855:901	The chitosan/gelatin-based composite film showed potent antimicrobial and antioxidant activities.
33984610	2	42	theme	compatible	358:367	arg1	film					353:356	a film	351:356	a film compatible with the chitosan/gelatin polymer matrix	351:408	The functional fillers were evenly distributed in the polymer matrix and formed a film compatible with the chitosan/gelatin polymer matrix.
33984610	3	43	theme	functional	424:433	arg1	material					435:442	the functional material	420:442	the functional material	420:442	However, the functional material did not affect the physical properties such as mechanical, thermal, and water vapor barrier properties of the film but exhibited high light transmittance with enhanced UV blocking properties.
33984610	3	44	theme	enhanced	603:610	arg1	properties					624:633	enhanced UV blocking properties	603:633	enhanced UV blocking properties	603:633	However, the functional material did not affect the physical properties such as mechanical, thermal, and water vapor barrier properties of the film but exhibited high light transmittance with enhanced UV blocking properties.
33984610	1	45	theme	Chitosan/gelatin-based	125:146	arg1	films					159:163	Chitosan/gelatin-based functional films	125:163	Chitosan/gelatin-based functional films	125:163	Chitosan/gelatin-based functional films were fabricated by incorporating cinnamon essential oil (CEO) and rutin for active packaging application.
33984610	6	46	theme	synergistic	960:970	arg1	effect					972:977	a synergistic effect	958:977	a synergistic effect of functional properties	958:1002	In addition, the combined use of CEO and rutin showed a synergistic effect of functional properties.
33984610	7	47	theme	functional	1085:1094	arg1	properties					1096:1105	improved physical and functional properties	1063:1105	improved physical and functional properties	1063:1105	The CEO and rutin-added chitosan/gelatin-based films with improved physical and functional properties can be used for active food packaging applications.
33984610	4	48	theme	various	698:704	arg1	solutions					720:728	various food simulant solutions	698:728	various food simulant solutions	698:728	The rutin release from the composite film was evaluated using various food simulant solutions and found that rutin was released faster in acidic and alcoholic solutions.
33984610	1	49	theme	functional	148:157	arg1	films					159:163	Chitosan/gelatin-based functional films	125:163	Chitosan/gelatin-based functional films	125:163	Chitosan/gelatin-based functional films were fabricated by incorporating cinnamon essential oil (CEO) and rutin for active packaging application.
33984610	7	50	theme	improved	1063:1070	arg1	properties					1096:1105	improved physical and functional properties	1063:1105	improved physical and functional properties	1063:1105	The CEO and rutin-added chitosan/gelatin-based films with improved physical and functional properties can be used for active food packaging applications.
33984610	2	51	theme	functional	275:284	arg1	fillers					286:292	The functional fillers	271:292	The functional fillers	271:292	The functional fillers were evenly distributed in the polymer matrix and formed a film compatible with the chitosan/gelatin polymer matrix.
33984610	7	52	theme	chitosan/gelatin-based	1029:1050	arg1	films					1052:1056	rutin-added chitosan/gelatin-based films	1017:1056	rutin-added chitosan/gelatin-based films	1017:1056	The CEO and rutin-added chitosan/gelatin-based films with improved physical and functional properties can be used for active food packaging applications.
33984610	3	53	theme	thermal	503:509	arg1	properties					536:545	mechanical, thermal, and water vapor barrier properties	491:545	mechanical, thermal, and water vapor barrier properties of the film	491:557	However, the functional material did not affect the physical properties such as mechanical, thermal, and water vapor barrier properties of the film but exhibited high light transmittance with enhanced UV blocking properties.
33984610	1	54	theme	active	241:246	arg1	application					258:268	active packaging application	241:268	active packaging application	241:268	Chitosan/gelatin-based functional films were fabricated by incorporating cinnamon essential oil (CEO) and rutin for active packaging application.
33984610	4	55	theme	rutin	640:644	arg1	release					646:652	The rutin release	636:652	The rutin release from the composite film	636:676	The rutin release from the composite film was evaluated using various food simulant solutions and found that rutin was released faster in acidic and alcoholic solutions.
33984610	3	56	theme	barrier	528:534	arg1	properties					536:545	mechanical, thermal, and water vapor barrier properties	491:545	mechanical, thermal, and water vapor barrier properties of the film	491:557	However, the functional material did not affect the physical properties such as mechanical, thermal, and water vapor barrier properties of the film but exhibited high light transmittance with enhanced UV blocking properties.
33984610	3	57	theme	physical	463:470	arg1	properties					472:481	the physical properties	459:481	the physical properties such as mechanical, thermal, and water vapor barrier properties of the film	459:557	However, the functional material did not affect the physical properties such as mechanical, thermal, and water vapor barrier properties of the film but exhibited high light transmittance with enhanced UV blocking properties.
33984610	3	57	theme	physical	463:470	arg1	properties					536:545	mechanical, thermal, and water vapor barrier properties	491:545	mechanical, thermal, and water vapor barrier properties of the film	491:557	However, the functional material did not affect the physical properties such as mechanical, thermal, and water vapor barrier properties of the film but exhibited high light transmittance with enhanced UV blocking properties.
33984610	1	58	theme	packaging	248:256	arg1	application					258:268	active packaging application	241:268	active packaging application	241:268	Chitosan/gelatin-based functional films were fabricated by incorporating cinnamon essential oil (CEO) and rutin for active packaging application.
33984610	3	59	with	transmittance	584:596	arg1	properties					624:633	enhanced UV blocking properties	603:633	enhanced UV blocking properties	603:633	However, the functional material did not affect the physical properties such as mechanical, thermal, and water vapor barrier properties of the film but exhibited high light transmittance with enhanced UV blocking properties.
33984610	2	60	theme	chitosan/gelatin	378:393	arg1	matrix					403:408	the chitosan/gelatin polymer matrix	374:408	the chitosan/gelatin polymer matrix	374:408	The functional fillers were evenly distributed in the polymer matrix and formed a film compatible with the chitosan/gelatin polymer matrix.
33984610	7	61	with	films	1052:1056	arg1	properties					1096:1105	improved physical and functional properties	1063:1105	improved physical and functional properties	1063:1105	The CEO and rutin-added chitosan/gelatin-based films with improved physical and functional properties can be used for active food packaging applications.
33652632	12	0	theme	fixation	1594:1601	arg1	plates					1603:1608	bioresorbable load-bearing composite fracture fixation plates	1548:1608	bioresorbable load-bearing composite fracture fixation plates	1548:1608	We concluded that PLAMA-500 has potential for the use as the matrix material for bioresorbable load-bearing composite fracture fixation plates.
33652632	4	1	theme	concept	877:883	arg1	prospective					852:862	prospective	852:862	prospective	852:862	The purpose was to obtain a preliminary understanding of the chemical and physical properties and the biological safety of PLAMA from the prospective of the novel concept.
33652632	6	2	theme	curing	995:1000	arg1	efficiency					981:990	The efficiency	977:990	The efficiency of curing	977:1000	The efficiency of curing was assessed by the degree of convergence (DC).
33652632	8	3	theme	body	1201:1204	arg1	fluid					1206:1210	simulated body fluid	1191:1210	simulated body fluid	1191:1210	The bioresorbability was investigated by immersion in simulated body fluid.
33652632	5	4	with	Modifications	886:898	arg1	PLAMA-1000					947:956	PLAMA-1000	947:956	PLAMA-1000	947:956	Modifications with different molecular masses (PLAMA-500 and PLAMA-1000) were synthesized.
33652632	5	4	with	Modifications	886:898	arg1	PLAMA-500					933:941	PLAMA-500	933:941	PLAMA-500	933:941	Modifications with different molecular masses (PLAMA-500 and PLAMA-1000) were synthesized.
33652632	5	4	with	Modifications	886:898	arg1	masses					925:930	different molecular masses	905:930	different molecular masses (PLAMA-500 and PLAMA-1000)	905:957	Modifications with different molecular masses (PLAMA-500 and PLAMA-1000) were synthesized.
33652632	12	5	theme	matrix	1528:1533	arg1	material					1535:1542	the matrix material	1524:1542	the matrix material for bioresorbable load-bearing composite fracture fixation plates	1524:1608	We concluded that PLAMA-500 has potential for the use as the matrix material for bioresorbable load-bearing composite fracture fixation plates.
33652632	11	6	theme	morphological	1431:1443	arg1	development					1445:1455	normal morphological development	1424:1455	normal morphological development	1424:1455	Both did not prevent proliferation and normal morphological development of cells.
33652632	1	7	theme	eliminated	344:353	arg1	need					355:358	eliminated need	344:358	eliminated need for plate removal	344:376	The use of bioresorbable fracture fixation plates made of aliphatic polyesters have good potential due to good biocompatibility, reduced risk of stress-shielding, and eliminated need for plate removal.
33652632	4	8	theme	biological	816:825	arg1	safety					827:832	the biological safety	812:832	the biological safety of PLAMA	812:841	The purpose was to obtain a preliminary understanding of the chemical and physical properties and the biological safety of PLAMA from the prospective of the novel concept.
33652632	4	9	theme	preliminary	742:752	arg1	understanding					754:766	a preliminary understanding	740:766	a preliminary understanding of the chemical and physical properties	740:806	The purpose was to obtain a preliminary understanding of the chemical and physical properties and the biological safety of PLAMA from the prospective of the novel concept.
33652632	11	10	theme	normal	1424:1429	arg1	development					1445:1455	normal morphological development	1424:1455	normal morphological development	1424:1455	Both did not prevent proliferation and normal morphological development of cells.
33652632	1	11	theme	good	261:264	arg1	potential					266:274	good potential	261:274	good potential	261:274	The use of bioresorbable fracture fixation plates made of aliphatic polyesters have good potential due to good biocompatibility, reduced risk of stress-shielding, and eliminated need for plate removal.
33652632	0	12	theme	In	60:61	arg1	Fiber					124:128	In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber	60:128	In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber	60:128	A Polymer for Application as a Matrix Phase in a Concept of In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber Reinforced Composite Fracture Fixation Plates.
33652632	0	13	from	Phase	38:42	arg1	Concept					49:55	a Concept	47:55	a Concept of In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber	47:128	A Polymer for Application as a Matrix Phase in a Concept of In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber Reinforced Composite Fracture Fixation Plates.
33652632	7	14	theme	tensile	1093:1099	arg1	test					1101:1104	tensile test	1093:1104	tensile test	1093:1104	The mechanical properties were obtained by tensile test and thermomechanical analysis.
33652632	5	15	dep	masses	925:930	arg1	PLAMA-1000					947:956	PLAMA-1000	947:956	PLAMA-1000	947:956	Modifications with different molecular masses (PLAMA-500 and PLAMA-1000) were synthesized.
33652632	5	15	dep	masses	925:930	arg1	PLAMA-500					933:941	PLAMA-500	933:941	PLAMA-500	933:941	Modifications with different molecular masses (PLAMA-500 and PLAMA-1000) were synthesized.
33652632	5	15	dep	masses	925:930	arg1	masses					925:930	different molecular masses	905:930	different molecular masses (PLAMA-500 and PLAMA-1000)	905:957	Modifications with different molecular masses (PLAMA-500 and PLAMA-1000) were synthesized.
33652632	12	16	theme	load-bearing	1562:1573	arg1	plates					1603:1608	bioresorbable load-bearing composite fracture fixation plates	1548:1608	bioresorbable load-bearing composite fracture fixation plates	1548:1608	We concluded that PLAMA-500 has potential for the use as the matrix material for bioresorbable load-bearing composite fracture fixation plates.
33652632	0	17	theme	Bioresorbable	76:88	arg1	Fiber					124:128	In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber	60:128	In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber	60:128	A Polymer for Application as a Matrix Phase in a Concept of In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber Reinforced Composite Fracture Fixation Plates.
33652632	6	18	theme	convergence	1032:1042	arg1	degree					1022:1027	the degree	1018:1027	the degree of convergence (DC)	1018:1047	The efficiency of curing was assessed by the degree of convergence (DC).
33652632	0	19	theme	Curable	68:74	arg1	Fiber					124:128	In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber	60:128	In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber	60:128	A Polymer for Application as a Matrix Phase in a Concept of In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber Reinforced Composite Fracture Fixation Plates.
33652632	1	20	theme	plate	364:368	arg1	removal					370:376	plate removal	364:376	plate removal	364:376	The use of bioresorbable fracture fixation plates made of aliphatic polyesters have good potential due to good biocompatibility, reduced risk of stress-shielding, and eliminated need for plate removal.
33652632	4	21	theme	physical	788:795	arg1	properties					797:806	the chemical and physical properties	771:806	the chemical and physical properties	771:806	The purpose was to obtain a preliminary understanding of the chemical and physical properties and the biological safety of PLAMA from the prospective of the novel concept.
33652632	12	22	theme	bioresorbable	1548:1560	arg1	plates					1603:1608	bioresorbable load-bearing composite fracture fixation plates	1548:1608	bioresorbable load-bearing composite fracture fixation plates	1548:1608	We concluded that PLAMA-500 has potential for the use as the matrix material for bioresorbable load-bearing composite fracture fixation plates.
33652632	0	23	dep	In	60:61	arg1	Situ					63:66	Situ	63:66	Situ	63:66	A Polymer for Application as a Matrix Phase in a Concept of In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber Reinforced Composite Fracture Fixation Plates.
33652632	3	24	theme	polyesters	702:711	arg1	limitations					674:684	the limitations	670:684	the limitations of conventional polyesters	670:711	We suggested an alternative, PLAMA (PolyLActide functionalized with diMethAcrylate), for the use as the matrix phase for the novel concept of the in situ curable bioresorbable load-bearing composite plate to reduce the limitations of conventional polyesters.
33652632	7	25	theme	mechanical	1054:1063	arg1	properties					1065:1074	The mechanical properties	1050:1074	The mechanical properties	1050:1074	The mechanical properties were obtained by tensile test and thermomechanical analysis.
33652632	3	26	dep	PLAMA	484:488	arg1	PolyLActide					491:501	PolyLActide	491:501	PolyLActide functionalized with diMethAcrylate	491:536	We suggested an alternative, PLAMA (PolyLActide functionalized with diMethAcrylate), for the use as the matrix phase for the novel concept of the in situ curable bioresorbable load-bearing composite plate to reduce the limitations of conventional polyesters.
33652632	11	27	theme	cells	1460:1464	arg1	proliferation					1406:1418	proliferation	1406:1418	proliferation	1406:1418	Both did not prevent proliferation and normal morphological development of cells.
33652632	11	27	theme	cells	1460:1464	arg1	development					1445:1455	normal morphological development	1424:1455	normal morphological development	1424:1455	Both did not prevent proliferation and normal morphological development of cells.
33652632	4	28	theme	PLAMA	837:841	arg1	safety					827:832	the biological safety	812:832	the biological safety of PLAMA	812:841	The purpose was to obtain a preliminary understanding of the chemical and physical properties and the biological safety of PLAMA from the prospective of the novel concept.
33652632	4	28	theme	PLAMA	837:841	arg1	understanding					754:766	a preliminary understanding	740:766	a preliminary understanding of the chemical and physical properties	740:806	The purpose was to obtain a preliminary understanding of the chemical and physical properties and the biological safety of PLAMA from the prospective of the novel concept.
33652632	2	29	theme	handling	422:429	arg1	properties					431:440	their handling properties	416:440	their handling properties	416:440	However, polyesters are ductile, and their handling properties are limited.
33652632	3	30	theme	novel	580:584	arg1	concept					586:592	the novel concept	576:592	the novel concept of the in situ curable bioresorbable load-bearing composite plate to reduce the limitations of conventional polyesters	576:711	We suggested an alternative, PLAMA (PolyLActide functionalized with diMethAcrylate), for the use as the matrix phase for the novel concept of the in situ curable bioresorbable load-bearing composite plate to reduce the limitations of conventional polyesters.
33652632	0	31	theme	Load-Bearing	100:111	arg1	Fiber					124:128	In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber	60:128	In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber	60:128	A Polymer for Application as a Matrix Phase in a Concept of In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber Reinforced Composite Fracture Fixation Plates.
33652632	10	32	theme	mechanical	1317:1326	arg1	properties					1328:1337	mechanical properties	1317:1337	mechanical properties	1317:1337	PLAMA-500 showed better DC and mechanical properties, and slower bioresorbability than PLAMA-1000.
33652632	8	33	theme	simulated	1191:1199	arg1	fluid					1206:1210	simulated body fluid	1191:1210	simulated body fluid	1191:1210	The bioresorbability was investigated by immersion in simulated body fluid.
33652632	1	34	theme	good	283:286	arg1	biocompatibility					288:303	good biocompatibility	283:303	good biocompatibility	283:303	The use of bioresorbable fracture fixation plates made of aliphatic polyesters have good potential due to good biocompatibility, reduced risk of stress-shielding, and eliminated need for plate removal.
33652632	0	35	theme	Bioactive	90:98	arg1	Fiber					124:128	In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber	60:128	In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber	60:128	A Polymer for Application as a Matrix Phase in a Concept of In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber Reinforced Composite Fracture Fixation Plates.
33652632	3	36	theme	curable	609:615	arg1	plate					654:658	the in situ curable bioresorbable load-bearing composite plate	597:658	the in situ curable bioresorbable load-bearing composite plate	597:658	We suggested an alternative, PLAMA (PolyLActide functionalized with diMethAcrylate), for the use as the matrix phase for the novel concept of the in situ curable bioresorbable load-bearing composite plate to reduce the limitations of conventional polyesters.
33652632	1	37	theme	bioresorbable	188:200	arg1	plates					220:225	bioresorbable fracture fixation plates	188:225	bioresorbable fracture fixation plates made of aliphatic polyesters	188:254	The use of bioresorbable fracture fixation plates made of aliphatic polyesters have good potential due to good biocompatibility, reduced risk of stress-shielding, and eliminated need for plate removal.
33652632	0	38	theme	Fiber	124:128	arg1	Concept					49:55	a Concept	47:55	a Concept of In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber	47:128	A Polymer for Application as a Matrix Phase in a Concept of In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber Reinforced Composite Fracture Fixation Plates.
33652632	3	39	theme	bioresorbable	617:629	arg1	plate					654:658	the in situ curable bioresorbable load-bearing composite plate	597:658	the in situ curable bioresorbable load-bearing composite plate	597:658	We suggested an alternative, PLAMA (PolyLActide functionalized with diMethAcrylate), for the use as the matrix phase for the novel concept of the in situ curable bioresorbable load-bearing composite plate to reduce the limitations of conventional polyesters.
33652632	10	40	dep	slower	1344:1349	arg1	bioresorbability					1351:1366	bioresorbability	1351:1366	bioresorbability	1351:1366	PLAMA-500 showed better DC and mechanical properties, and slower bioresorbability than PLAMA-1000.
33652632	1	41	theme	fracture	202:209	arg1	plates					220:225	bioresorbable fracture fixation plates	188:225	bioresorbable fracture fixation plates made of aliphatic polyesters	188:254	The use of bioresorbable fracture fixation plates made of aliphatic polyesters have good potential due to good biocompatibility, reduced risk of stress-shielding, and eliminated need for plate removal.
33652632	0	42	theme	Continuous	113:122	arg1	Fiber					124:128	In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber	60:128	In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber	60:128	A Polymer for Application as a Matrix Phase in a Concept of In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber Reinforced Composite Fracture Fixation Plates.
33652632	3	43	theme	in	601:602	arg1	plate					654:658	the in situ curable bioresorbable load-bearing composite plate	597:658	the in situ curable bioresorbable load-bearing composite plate	597:658	We suggested an alternative, PLAMA (PolyLActide functionalized with diMethAcrylate), for the use as the matrix phase for the novel concept of the in situ curable bioresorbable load-bearing composite plate to reduce the limitations of conventional polyesters.
33652632	4	44	theme	properties	797:806	arg1	safety					827:832	the biological safety	812:832	the biological safety of PLAMA	812:841	The purpose was to obtain a preliminary understanding of the chemical and physical properties and the biological safety of PLAMA from the prospective of the novel concept.
33652632	4	44	theme	properties	797:806	arg1	understanding					754:766	a preliminary understanding	740:766	a preliminary understanding of the chemical and physical properties	740:806	The purpose was to obtain a preliminary understanding of the chemical and physical properties and the biological safety of PLAMA from the prospective of the novel concept.
33652632	1	45	theme	fixation	211:218	arg1	plates					220:225	bioresorbable fracture fixation plates	188:225	bioresorbable fracture fixation plates made of aliphatic polyesters	188:254	The use of bioresorbable fracture fixation plates made of aliphatic polyesters have good potential due to good biocompatibility, reduced risk of stress-shielding, and eliminated need for plate removal.
33652632	7	46	theme	thermomechanical	1110:1125	arg1	analysis					1127:1134	thermomechanical analysis	1110:1134	thermomechanical analysis	1110:1134	The mechanical properties were obtained by tensile test and thermomechanical analysis.
33652632	1	47	theme	reduced	306:312	arg1	risk					314:317	reduced risk	306:317	reduced risk of stress-shielding	306:337	The use of bioresorbable fracture fixation plates made of aliphatic polyesters have good potential due to good biocompatibility, reduced risk of stress-shielding, and eliminated need for plate removal.
33652632	0	48	theme	Composite	141:149	arg1	Plates					169:174	Composite Fracture Fixation Plates	141:174	Composite Fracture Fixation Plates	141:174	A Polymer for Application as a Matrix Phase in a Concept of In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber Reinforced Composite Fracture Fixation Plates.
33652632	9	49	theme	cell	1249:1252	arg1	morphology					1254:1263	cell morphology	1249:1263	cell morphology	1249:1263	The biocompatibility was studied in cell morphology and viability tests.
33652632	3	50	theme	matrix	559:564	arg1	phase					566:570	the matrix phase	555:570	the matrix phase for the novel concept of the in situ curable bioresorbable load-bearing composite plate to reduce the limitations of conventional polyesters	555:711	We suggested an alternative, PLAMA (PolyLActide functionalized with diMethAcrylate), for the use as the matrix phase for the novel concept of the in situ curable bioresorbable load-bearing composite plate to reduce the limitations of conventional polyesters.
33652632	3	51	theme	conventional	689:700	arg1	polyesters					702:711	conventional polyesters	689:711	conventional polyesters	689:711	We suggested an alternative, PLAMA (PolyLActide functionalized with diMethAcrylate), for the use as the matrix phase for the novel concept of the in situ curable bioresorbable load-bearing composite plate to reduce the limitations of conventional polyesters.
33652632	12	52	theme	composite	1575:1583	arg1	plates					1603:1608	bioresorbable load-bearing composite fracture fixation plates	1548:1608	bioresorbable load-bearing composite fracture fixation plates	1548:1608	We concluded that PLAMA-500 has potential for the use as the matrix material for bioresorbable load-bearing composite fracture fixation plates.
33652632	0	53	theme	Matrix	31:36	arg1	Phase					38:42	a Matrix Phase	29:42	a Matrix Phase in a Concept of In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber	29:128	A Polymer for Application as a Matrix Phase in a Concept of In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber Reinforced Composite Fracture Fixation Plates.
33652632	4	54	theme	novel	871:875	arg1	concept					877:883	the novel concept	867:883	the novel concept	867:883	The purpose was to obtain a preliminary understanding of the chemical and physical properties and the biological safety of PLAMA from the prospective of the novel concept.
33652632	3	55	theme	plate	654:658	arg1	concept					586:592	the novel concept	576:592	the novel concept of the in situ curable bioresorbable load-bearing composite plate to reduce the limitations of conventional polyesters	576:711	We suggested an alternative, PLAMA (PolyLActide functionalized with diMethAcrylate), for the use as the matrix phase for the novel concept of the in situ curable bioresorbable load-bearing composite plate to reduce the limitations of conventional polyesters.
33652632	5	56	theme	different	905:913	arg1	PLAMA-1000					947:956	PLAMA-1000	947:956	PLAMA-1000	947:956	Modifications with different molecular masses (PLAMA-500 and PLAMA-1000) were synthesized.
33652632	5	56	theme	different	905:913	arg1	PLAMA-500					933:941	PLAMA-500	933:941	PLAMA-500	933:941	Modifications with different molecular masses (PLAMA-500 and PLAMA-1000) were synthesized.
33652632	5	56	theme	different	905:913	arg1	masses					925:930	different molecular masses	905:930	different molecular masses (PLAMA-500 and PLAMA-1000)	905:957	Modifications with different molecular masses (PLAMA-500 and PLAMA-1000) were synthesized.
33652632	0	57	theme	Fixation	160:167	arg1	Plates					169:174	Composite Fracture Fixation Plates	141:174	Composite Fracture Fixation Plates	141:174	A Polymer for Application as a Matrix Phase in a Concept of In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber Reinforced Composite Fracture Fixation Plates.
33652632	4	58	theme	chemical	775:782	arg1	properties					797:806	the chemical and physical properties	771:806	the chemical and physical properties	771:806	The purpose was to obtain a preliminary understanding of the chemical and physical properties and the biological safety of PLAMA from the prospective of the novel concept.
33652632	5	59	theme	molecular	915:923	arg1	PLAMA-1000					947:956	PLAMA-1000	947:956	PLAMA-1000	947:956	Modifications with different molecular masses (PLAMA-500 and PLAMA-1000) were synthesized.
33652632	5	59	theme	molecular	915:923	arg1	PLAMA-500					933:941	PLAMA-500	933:941	PLAMA-500	933:941	Modifications with different molecular masses (PLAMA-500 and PLAMA-1000) were synthesized.
33652632	5	59	theme	molecular	915:923	arg1	masses					925:930	different molecular masses	905:930	different molecular masses (PLAMA-500 and PLAMA-1000)	905:957	Modifications with different molecular masses (PLAMA-500 and PLAMA-1000) were synthesized.
33652632	1	60	theme	plates	220:225	arg1	use					181:183	The use	177:183	The use of bioresorbable fracture fixation plates made of aliphatic polyesters	177:254	The use of bioresorbable fracture fixation plates made of aliphatic polyesters have good potential due to good biocompatibility, reduced risk of stress-shielding, and eliminated need for plate removal.
33652632	0	61	theme	Fracture	151:158	arg1	Plates					169:174	Composite Fracture Fixation Plates	141:174	Composite Fracture Fixation Plates	141:174	A Polymer for Application as a Matrix Phase in a Concept of In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber Reinforced Composite Fracture Fixation Plates.
33652632	3	62	theme	load-bearing	631:642	arg1	plate					654:658	the in situ curable bioresorbable load-bearing composite plate	597:658	the in situ curable bioresorbable load-bearing composite plate	597:658	We suggested an alternative, PLAMA (PolyLActide functionalized with diMethAcrylate), for the use as the matrix phase for the novel concept of the in situ curable bioresorbable load-bearing composite plate to reduce the limitations of conventional polyesters.
33652632	12	63	contain	has	1495:1497	arg2	potential					1499:1507	potential	1499:1507	potential for the use as the matrix material for bioresorbable load-bearing composite fracture fixation plates	1499:1608	We concluded that PLAMA-500 has potential for the use as the matrix material for bioresorbable load-bearing composite fracture fixation plates.
33652632	12	63	contain	has	1495:1497	arg1	PLAMA-500					1485:1493	PLAMA-500	1485:1493	PLAMA-500	1485:1493	We concluded that PLAMA-500 has potential for the use as the matrix material for bioresorbable load-bearing composite fracture fixation plates.
33652632	12	64	theme	fracture	1585:1592	arg1	plates					1603:1608	bioresorbable load-bearing composite fracture fixation plates	1548:1608	bioresorbable load-bearing composite fracture fixation plates	1548:1608	We concluded that PLAMA-500 has potential for the use as the matrix material for bioresorbable load-bearing composite fracture fixation plates.
33652632	3	65	theme	composite	644:652	arg1	plate					654:658	the in situ curable bioresorbable load-bearing composite plate	597:658	the in situ curable bioresorbable load-bearing composite plate	597:658	We suggested an alternative, PLAMA (PolyLActide functionalized with diMethAcrylate), for the use as the matrix phase for the novel concept of the in situ curable bioresorbable load-bearing composite plate to reduce the limitations of conventional polyesters.
33652632	1	66	contain	have	256:259	arg2	potential					266:274	good potential	261:274	good potential	261:274	The use of bioresorbable fracture fixation plates made of aliphatic polyesters have good potential due to good biocompatibility, reduced risk of stress-shielding, and eliminated need for plate removal.
33652632	1	66	contain	have	256:259	arg1	use					181:183	The use	177:183	The use of bioresorbable fracture fixation plates made of aliphatic polyesters	177:254	The use of bioresorbable fracture fixation plates made of aliphatic polyesters have good potential due to good biocompatibility, reduced risk of stress-shielding, and eliminated need for plate removal.
33652632	9	67	dep	morphology	1254:1263	arg1	tests					1279:1283	tests	1279:1283	tests	1279:1283	The biocompatibility was studied in cell morphology and viability tests.
33652632	1	68	theme	stress-shielding	322:337	arg1	biocompatibility					288:303	good biocompatibility	283:303	good biocompatibility	283:303	The use of bioresorbable fracture fixation plates made of aliphatic polyesters have good potential due to good biocompatibility, reduced risk of stress-shielding, and eliminated need for plate removal.
33652632	1	68	theme	stress-shielding	322:337	arg1	risk					314:317	reduced risk	306:317	reduced risk of stress-shielding	306:337	The use of bioresorbable fracture fixation plates made of aliphatic polyesters have good potential due to good biocompatibility, reduced risk of stress-shielding, and eliminated need for plate removal.
33652632	1	68	theme	stress-shielding	322:337	arg1	need					355:358	eliminated need	344:358	eliminated need for plate removal	344:376	The use of bioresorbable fracture fixation plates made of aliphatic polyesters have good potential due to good biocompatibility, reduced risk of stress-shielding, and eliminated need for plate removal.
33652632	10	69	dep	better	1303:1308	arg1	DC					1310:1311	DC	1310:1311	DC	1310:1311	PLAMA-500 showed better DC and mechanical properties, and slower bioresorbability than PLAMA-1000.
33652632	10	69	dep	better	1303:1308	arg1	properties					1328:1337	mechanical properties	1317:1337	mechanical properties	1317:1337	PLAMA-500 showed better DC and mechanical properties, and slower bioresorbability than PLAMA-1000.
33652632	3	70	dep	in	601:602	arg1	situ					604:607	situ	604:607	situ	604:607	We suggested an alternative, PLAMA (PolyLActide functionalized with diMethAcrylate), for the use as the matrix phase for the novel concept of the in situ curable bioresorbable load-bearing composite plate to reduce the limitations of conventional polyesters.
33652632	1	71	theme	aliphatic	235:243	arg1	polyesters					245:254	aliphatic polyesters	235:254	aliphatic polyesters	235:254	The use of bioresorbable fracture fixation plates made of aliphatic polyesters have good potential due to good biocompatibility, reduced risk of stress-shielding, and eliminated need for plate removal.
34146360	7	0	theme	comparable	1140:1149	arg1	actions					1164:1170	potent comparable bactericidal actions	1133:1170	potent comparable bactericidal actions	1133:1170	The NPs (plain Ag NPs and composited βG-Ag NPs) exhibited potent comparable bactericidal actions, opposing Gram+ (Staphylococcus aureus) and Gram- (Escherichia coli, Salmonella Typhimurium, and Pseudomonas aeruginosa).
34146360	5	1	from	interaction	846:856	arg1	NPs					896:898	βG NPs	893:898	βG NPs	893:898	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	7	2	theme	composited	1101:1110	arg1	NPs					1118:1120	composited βG-Ag NPs	1101:1120	composited βG-Ag NPs	1101:1120	The NPs (plain Ag NPs and composited βG-Ag NPs) exhibited potent comparable bactericidal actions, opposing Gram+ (Staphylococcus aureus) and Gram- (Escherichia coli, Salmonella Typhimurium, and Pseudomonas aeruginosa).
34146360	3	3	from	cerevisiae	474:483	arg1	βG					450:451	βG	450:451	βG	450:451	Yeast β-glucan (βG), from Saccharomyces cerevisiae, was extracted and transformed to nanoparticles (NPs) using alkali/acid facile protocol.
34146360	3	3	from	cerevisiae	474:483	arg1	β-glucan					440:447	Yeast β-glucan	434:447	Yeast β-glucan (βG)	434:452	Yeast β-glucan (βG), from Saccharomyces cerevisiae, was extracted and transformed to nanoparticles (NPs) using alkali/acid facile protocol.
34146360	1	4	theme	potent	165:170	arg1	potentials					186:195	potent antimicrobial potentials	165:195	potent antimicrobial potentials	165:195	Nanometals (NM) frequently possess potent antimicrobial potentials to combat various pathogens, but their elevated biotoxicity limits their direct applications.
34146360	2	5	with	capping/conjugation	324:342	arg1	biopolymers					357:367	natural biopolymers	349:367	natural biopolymers	349:367	The biosynthesis of NM and their capping/conjugation with natural biopolymers can effectually enhance NM stability and diminish such toxicity.
34146360	5	6	theme	infrared	754:761	arg1	assessments					723:733	The physicochemical assessments	703:733	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy)	703:819	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	5	6	theme	infrared	754:761	arg1	diffraction					770:780	X-ray diffraction	764:780	X-ray diffraction	764:780	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	7	7	theme	βG-Ag	1112:1116	arg1	NPs					1118:1120	composited βG-Ag NPs	1101:1120	composited βG-Ag NPs	1101:1120	The NPs (plain Ag NPs and composited βG-Ag NPs) exhibited potent comparable bactericidal actions, opposing Gram+ (Staphylococcus aureus) and Gram- (Escherichia coli, Salmonella Typhimurium, and Pseudomonas aeruginosa).
34146360	2	8	theme	NM	393:394	arg1	stability					396:404	NM stability	393:404	NM stability	393:404	The biosynthesis of NM and their capping/conjugation with natural biopolymers can effectually enhance NM stability and diminish such toxicity.
34146360	0	9	theme	antibacterial	105:117	arg1	composite					119:127	antibacterial composite	105:127	antibacterial composite	105:127	Innovative biosynthesis of silver nanoparticles using yeast glucan nanopolymer and their potentiality as antibacterial composite.
34146360	5	10	from	formation	835:843	arg1	NPs					896:898	βG NPs	893:898	βG NPs	893:898	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	9	11	theme	βG-Ag	1531:1535	arg1	nanocomposites					1541:1554	the combined βG-Ag NPs nanocomposites	1518:1554	the combined βG-Ag NPs nanocomposites	1518:1554	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites could be impressively recommended as powerful antibacterial candidates with minor potential toxicity.
34146360	8	12	theme	Typhimurium	1344:1354	arg1	micrographs					1303:1313	Scanning micrographs	1294:1313	Scanning micrographs	1294:1313	Scanning micrographs, of treated S. aureus and S. Typhimurium with βG-Ag NPs, elucidated the powerful bactericidal actions of nanocomposite for destructing pathogens' cells.
34146360	6	13	theme	minute	989:994	arg1	particle sizes					996:1009	minute particle sizes	989:1009	minute particle sizes	989:1009	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	2	14	theme	NM	311:312	arg1	capping/conjugation					324:342	their capping/conjugation	318:342	their capping/conjugation with natural biopolymers	318:367	The biosynthesis of NM and their capping/conjugation with natural biopolymers can effectually enhance NM stability and diminish such toxicity.
34146360	2	14	theme	NM	311:312	arg1	biosynthesis					295:306	The biosynthesis	291:306	The biosynthesis of NM	291:312	The biosynthesis of NM and their capping/conjugation with natural biopolymers can effectually enhance NM stability and diminish such toxicity.
34146360	4	15	theme	direct	616:621	arg1	biosynthesis					623:634	direct biosynthesis	616:634	direct biosynthesis of silver nanoparticles (Ag NPs) without extra chemical processes	616:700	The βG NPs were innovatively employed for direct biosynthesis of silver nanoparticles (Ag NPs) without extra chemical processes.
34146360	5	16	from	capping	872:878	arg1	NPs					896:898	βG NPs	893:898	βG NPs	893:898	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	7	17	theme	plain	1084:1088	arg1	NPs					1093:1095	plain Ag NPs	1084:1095	plain Ag NPs	1084:1095	The NPs (plain Ag NPs and composited βG-Ag NPs) exhibited potent comparable bactericidal actions, opposing Gram+ (Staphylococcus aureus) and Gram- (Escherichia coli, Salmonella Typhimurium, and Pseudomonas aeruginosa).
34146360	5	18	theme	Ag	883:884	arg1	NPs					886:888	Ag NPs	883:888	Ag NPs	883:888	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	3	19	theme	facile	557:562	arg1	protocol					564:571	alkali/acid facile protocol	545:571	alkali/acid facile protocol	545:571	Yeast β-glucan (βG), from Saccharomyces cerevisiae, was extracted and transformed to nanoparticles (NPs) using alkali/acid facile protocol.
34146360	4	20	theme	chemical	683:690	arg1	processes					692:700	extra chemical processes	677:700	extra chemical processes	677:700	The βG NPs were innovatively employed for direct biosynthesis of silver nanoparticles (Ag NPs) without extra chemical processes.
34146360	5	21	theme	transmission	787:798	arg1	assessments					723:733	The physicochemical assessments	703:733	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy)	703:819	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	5	21	theme	transmission	787:798	arg1	microscopy					809:818	transmission electron microscopy	787:818	transmission electron microscopy	787:818	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	1	22	contain	possess	157:163	arg1	NM					142:143	NM	142:143	NM	142:143	Nanometals (NM) frequently possess potent antimicrobial potentials to combat various pathogens, but their elevated biotoxicity limits their direct applications.
34146360	1	22	contain	possess	157:163	arg2	potentials					186:195	potent antimicrobial potentials	165:195	potent antimicrobial potentials	165:195	Nanometals (NM) frequently possess potent antimicrobial potentials to combat various pathogens, but their elevated biotoxicity limits their direct applications.
34146360	1	22	contain	possess	157:163	arg1	Nanometals					130:139	Nanometals	130:139	Nanometals (NM)	130:144	Nanometals (NM) frequently possess potent antimicrobial potentials to combat various pathogens, but their elevated biotoxicity limits their direct applications.
34146360	1	23	theme	direct	270:275	arg1	applications					277:288	their direct applications	264:288	their direct applications	264:288	Nanometals (NM) frequently possess potent antimicrobial potentials to combat various pathogens, but their elevated biotoxicity limits their direct applications.
34146360	9	24	with	candidates	1616:1625	arg1	toxicity					1648:1655	minor potential toxicity	1632:1655	minor potential toxicity	1632:1655	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites could be impressively recommended as powerful antibacterial candidates with minor potential toxicity.
34146360	0	25	theme	Innovative	0:9	arg1	biosynthesis					11:22	Innovative biosynthesis	0:22	Innovative biosynthesis of silver nanoparticles using yeast glucan nanopolymer and their potentiality as antibacterial composite.	0:128	Innovative biosynthesis of silver nanoparticles using yeast glucan nanopolymer and their potentiality as antibacterial composite.
34146360	4	26	theme	extra	677:681	arg1	processes					692:700	extra chemical processes	677:700	extra chemical processes	677:700	The βG NPs were innovatively employed for direct biosynthesis of silver nanoparticles (Ag NPs) without extra chemical processes.
34146360	6	27	theme	synthesized	905:915	arg1	NPs					920:922	NPs	920:922	NPs	920:922	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	6	27	theme	synthesized	905:915	arg1	NPs					928:930	Ag NPs	925:930	Ag NPs	925:930	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	6	27	theme	synthesized	905:915	arg1	βG					917:918	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite	901:955	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite	901:955	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	6	27	theme	synthesized	905:915	arg1	composite					947:955	βG-Ag NPs composite	937:955	βG-Ag NPs composite	937:955	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	6	27	theme	synthesized	905:915	arg1	charged					973:979	charged	973:979	charged	973:979	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	9	28	theme	NPs	1537:1539	arg1	nanocomposites					1541:1554	the combined βG-Ag NPs nanocomposites	1518:1554	the combined βG-Ag NPs nanocomposites	1518:1554	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites could be impressively recommended as powerful antibacterial candidates with minor potential toxicity.
34146360	2	29	theme	natural	349:355	arg1	biopolymers					357:367	natural biopolymers	349:367	natural biopolymers	349:367	The biosynthesis of NM and their capping/conjugation with natural biopolymers can effectually enhance NM stability and diminish such toxicity.
34146360	7	30	dep	Gram+	1182:1186	arg1	aureus					1204:1209	Staphylococcus aureus	1189:1209	Staphylococcus aureus	1189:1209	The NPs (plain Ag NPs and composited βG-Ag NPs) exhibited potent comparable bactericidal actions, opposing Gram+ (Staphylococcus aureus) and Gram- (Escherichia coli, Salmonella Typhimurium, and Pseudomonas aeruginosa).
34146360	3	31	theme	Yeast	434:438	arg1	βG					450:451	βG	450:451	βG	450:451	Yeast β-glucan (βG), from Saccharomyces cerevisiae, was extracted and transformed to nanoparticles (NPs) using alkali/acid facile protocol.
34146360	3	31	theme	Yeast	434:438	arg1	β-glucan					440:447	Yeast β-glucan	434:447	Yeast β-glucan (βG)	434:452	Yeast β-glucan (βG), from Saccharomyces cerevisiae, was extracted and transformed to nanoparticles (NPs) using alkali/acid facile protocol.
34146360	7	32	theme	potent	1133:1138	arg1	actions					1164:1170	potent comparable bactericidal actions	1133:1170	potent comparable bactericidal actions	1133:1170	The NPs (plain Ag NPs and composited βG-Ag NPs) exhibited potent comparable bactericidal actions, opposing Gram+ (Staphylococcus aureus) and Gram- (Escherichia coli, Salmonella Typhimurium, and Pseudomonas aeruginosa).
34146360	5	33	theme	βG	893:894	arg1	NPs					896:898	βG NPs	893:898	βG NPs	893:898	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	6	34	theme	mean	1016:1019	arg1	diameters					1021:1029	mean diameters	1016:1029	mean diameters of 58.65, 6.72, and 63.88 nm, respectively	1016:1072	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	2	35	theme	such	419:422	arg1	toxicity					424:431	such toxicity	419:431	such toxicity	419:431	The biosynthesis of NM and their capping/conjugation with natural biopolymers can effectually enhance NM stability and diminish such toxicity.
34146360	0	36	theme	silver	27:32	arg1	nanoparticles					34:46	silver nanoparticles	27:46	silver nanoparticles	27:46	Innovative biosynthesis of silver nanoparticles using yeast glucan nanopolymer and their potentiality as antibacterial composite.
34146360	4	37	theme	βG	578:579	arg1	NPs					581:583	The βG NPs	574:583	The βG NPs	574:583	The βG NPs were innovatively employed for direct biosynthesis of silver nanoparticles (Ag NPs) without extra chemical processes.
34146360	5	38	theme	Fourier-transform	736:752	arg1	assessments					723:733	The physicochemical assessments	703:733	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy)	703:819	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	5	38	theme	Fourier-transform	736:752	arg1	diffraction					770:780	X-ray diffraction	764:780	X-ray diffraction	764:780	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	9	39	theme	antibacterial	1602:1614	arg1	biosynthesis					1489:1500	The inventive Ag NPs biosynthesis	1468:1500	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites	1468:1554	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites could be impressively recommended as powerful antibacterial candidates with minor potential toxicity.
34146360	9	39	theme	antibacterial	1602:1614	arg1	candidates					1616:1625	powerful antibacterial candidates	1593:1625	powerful antibacterial candidates with minor potential toxicity	1593:1655	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites could be impressively recommended as powerful antibacterial candidates with minor potential toxicity.
34146360	8	40	theme	βG-Ag	1361:1365	arg1	NPs					1367:1369	βG-Ag NPs	1361:1369	βG-Ag NPs	1361:1369	Scanning micrographs, of treated S. aureus and S. Typhimurium with βG-Ag NPs, elucidated the powerful bactericidal actions of nanocomposite for destructing pathogens' cells.
34146360	7	41	theme	Ag	1090:1091	arg1	NPs					1093:1095	plain Ag NPs	1084:1095	plain Ag NPs	1084:1095	The NPs (plain Ag NPs and composited βG-Ag NPs) exhibited potent comparable bactericidal actions, opposing Gram+ (Staphylococcus aureus) and Gram- (Escherichia coli, Salmonella Typhimurium, and Pseudomonas aeruginosa).
34146360	6	42	theme	63.88 nm	1051:1058	arg1	diameters					1021:1029	mean diameters	1016:1029	mean diameters of 58.65, 6.72, and 63.88 nm, respectively	1016:1072	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	7	43	theme	bactericidal	1151:1162	arg1	actions					1164:1170	potent comparable bactericidal actions	1133:1170	potent comparable bactericidal actions	1133:1170	The NPs (plain Ag NPs and composited βG-Ag NPs) exhibited potent comparable bactericidal actions, opposing Gram+ (Staphylococcus aureus) and Gram- (Escherichia coli, Salmonella Typhimurium, and Pseudomonas aeruginosa).
34146360	4	44	theme	silver	639:644	arg1	nanoparticles					646:658	silver nanoparticles	639:658	silver nanoparticles (Ag NPs) without extra chemical processes	639:700	The βG NPs were innovatively employed for direct biosynthesis of silver nanoparticles (Ag NPs) without extra chemical processes.
34146360	4	44	theme	silver	639:644	arg1	NPs					664:666	Ag NPs	661:666	Ag NPs	661:666	The βG NPs were innovatively employed for direct biosynthesis of silver nanoparticles (Ag NPs) without extra chemical processes.
34146360	1	45	theme	antimicrobial	172:184	arg1	potentials					186:195	potent antimicrobial potentials	165:195	potent antimicrobial potentials	165:195	Nanometals (NM) frequently possess potent antimicrobial potentials to combat various pathogens, but their elevated biotoxicity limits their direct applications.
34146360	4	46	theme	Ag	661:662	arg1	nanoparticles					646:658	silver nanoparticles	639:658	silver nanoparticles (Ag NPs) without extra chemical processes	639:700	The βG NPs were innovatively employed for direct biosynthesis of silver nanoparticles (Ag NPs) without extra chemical processes.
34146360	4	46	theme	Ag	661:662	arg1	NPs					664:666	Ag NPs	661:666	Ag NPs	661:666	The βG NPs were innovatively employed for direct biosynthesis of silver nanoparticles (Ag NPs) without extra chemical processes.
34146360	5	47	theme	NPs	831:833	arg1	formation					835:843	NPs formation	831:843	NPs formation	831:843	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	8	48	theme	nanocomposite	1420:1432	arg1	actions					1409:1415	the powerful bactericidal actions	1383:1415	the powerful bactericidal actions of nanocomposite for destructing pathogens' cells	1383:1465	Scanning micrographs, of treated S. aureus and S. Typhimurium with βG-Ag NPs, elucidated the powerful bactericidal actions of nanocomposite for destructing pathogens' cells.
34146360	6	49	dep	βG	917:918	arg1	NPs					920:922	NPs	920:922	NPs	920:922	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	6	49	dep	βG	917:918	arg1	NPs					928:930	Ag NPs	925:930	Ag NPs	925:930	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	6	49	dep	βG	917:918	arg1	βG					917:918	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite	901:955	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite	901:955	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	6	49	dep	βG	917:918	arg1	composite					947:955	βG-Ag NPs composite	937:955	βG-Ag NPs composite	937:955	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	1	50	theme	various	207:213	arg1	pathogens					215:223	various pathogens	207:223	various pathogens	207:223	Nanometals (NM) frequently possess potent antimicrobial potentials to combat various pathogens, but their elevated biotoxicity limits their direct applications.
34146360	5	51	theme	NPs	886:888	arg1	interaction					846:856	interaction	846:856	interaction	846:856	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	5	51	theme	NPs	886:888	arg1	formation					835:843	NPs formation	831:843	NPs formation	831:843	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	5	51	theme	NPs	886:888	arg1	capping					872:878	interior capping	863:878	interior capping	863:878	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	0	52	theme	nanoparticles	34:46	arg1	biosynthesis					11:22	Innovative biosynthesis	0:22	Innovative biosynthesis of silver nanoparticles using yeast glucan nanopolymer and their potentiality as antibacterial composite.	0:128	Innovative biosynthesis of silver nanoparticles using yeast glucan nanopolymer and their potentiality as antibacterial composite.
34146360	9	53	with	biosynthesis	1489:1500	arg1	NPs					1510:1512	βG NPs	1507:1512	βG NPs	1507:1512	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites could be impressively recommended as powerful antibacterial candidates with minor potential toxicity.
34146360	9	53	with	biosynthesis	1489:1500	arg1	nanocomposites					1541:1554	the combined βG-Ag NPs nanocomposites	1518:1554	the combined βG-Ag NPs nanocomposites	1518:1554	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites could be impressively recommended as powerful antibacterial candidates with minor potential toxicity.
34146360	5	54	theme	X-ray	764:768	arg1	assessments					723:733	The physicochemical assessments	703:733	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy)	703:819	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	5	54	theme	X-ray	764:768	arg1	diffraction					770:780	X-ray diffraction	764:780	X-ray diffraction	764:780	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	5	55	theme	interior	863:870	arg1	capping					872:878	interior capping	863:878	interior capping	863:878	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	9	56	theme	minor	1632:1636	arg1	toxicity					1648:1655	minor potential toxicity	1632:1655	minor potential toxicity	1632:1655	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites could be impressively recommended as powerful antibacterial candidates with minor potential toxicity.
34146360	6	57	theme	6.72	1041:1044	arg1	diameters					1021:1029	mean diameters	1016:1029	mean diameters of 58.65, 6.72, and 63.88 nm, respectively	1016:1072	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	0	58	theme	glucan	60:65	arg1	nanopolymer					67:77	yeast glucan nanopolymer	54:77	yeast glucan nanopolymer	54:77	Innovative biosynthesis of silver nanoparticles using yeast glucan nanopolymer and their potentiality as antibacterial composite.
34146360	5	59	dep	assessments	723:733	arg1	assessments					723:733	The physicochemical assessments	703:733	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy)	703:819	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	5	59	dep	assessments	723:733	arg1	microscopy					809:818	transmission electron microscopy	787:818	transmission electron microscopy	787:818	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	5	59	dep	assessments	723:733	arg1	diffraction					770:780	X-ray diffraction	764:780	X-ray diffraction	764:780	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	9	60	theme	βG	1507:1508	arg1	NPs					1510:1512	βG NPs	1507:1512	βG NPs	1507:1512	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites could be impressively recommended as powerful antibacterial candidates with minor potential toxicity.
34146360	9	61	theme	powerful	1593:1600	arg1	biosynthesis					1489:1500	The inventive Ag NPs biosynthesis	1468:1500	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites	1468:1554	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites could be impressively recommended as powerful antibacterial candidates with minor potential toxicity.
34146360	9	61	theme	powerful	1593:1600	arg1	candidates					1616:1625	powerful antibacterial candidates	1593:1625	powerful antibacterial candidates with minor potential toxicity	1593:1655	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites could be impressively recommended as powerful antibacterial candidates with minor potential toxicity.
34146360	9	62	theme	inventive	1472:1480	arg1	biosynthesis					1489:1500	The inventive Ag NPs biosynthesis	1468:1500	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites	1468:1554	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites could be impressively recommended as powerful antibacterial candidates with minor potential toxicity.
34146360	9	62	theme	inventive	1472:1480	arg1	candidates					1616:1625	powerful antibacterial candidates	1593:1625	powerful antibacterial candidates with minor potential toxicity	1593:1655	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites could be impressively recommended as powerful antibacterial candidates with minor potential toxicity.
34146360	0	63	theme	yeast	54:58	arg1	nanopolymer					67:77	yeast glucan nanopolymer	54:77	yeast glucan nanopolymer	54:77	Innovative biosynthesis of silver nanoparticles using yeast glucan nanopolymer and their potentiality as antibacterial composite.
34146360	6	64	theme	NPs	943:945	arg1	composite					947:955	βG-Ag NPs composite	937:955	βG-Ag NPs composite	937:955	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	6	64	theme	NPs	943:945	arg1	βG					917:918	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite	901:955	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite	901:955	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	5	65	theme	physicochemical	707:721	arg1	assessments					723:733	The physicochemical assessments	703:733	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy)	703:819	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	5	65	theme	physicochemical	707:721	arg1	microscopy					809:818	transmission electron microscopy	787:818	transmission electron microscopy	787:818	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	5	65	theme	physicochemical	707:721	arg1	diffraction					770:780	X-ray diffraction	764:780	X-ray diffraction	764:780	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	6	66	contain	had	985:987	arg1	NPs					928:930	Ag NPs	925:930	Ag NPs	925:930	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	6	66	contain	had	985:987	arg1	charged					973:979	charged	973:979	charged	973:979	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	6	66	contain	had	985:987	arg1	βG					917:918	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite	901:955	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite	901:955	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	6	66	contain	had	985:987	arg2	particle sizes					996:1009	minute particle sizes	989:1009	minute particle sizes	989:1009	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	6	66	contain	had	985:987	arg1	NPs					920:922	NPs	920:922	NPs	920:922	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	6	66	contain	had	985:987	arg1	composite					947:955	βG-Ag NPs composite	937:955	βG-Ag NPs composite	937:955	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	9	67	theme	Ag	1482:1483	arg1	biosynthesis					1489:1500	The inventive Ag NPs biosynthesis	1468:1500	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites	1468:1554	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites could be impressively recommended as powerful antibacterial candidates with minor potential toxicity.
34146360	9	67	theme	Ag	1482:1483	arg1	candidates					1616:1625	powerful antibacterial candidates	1593:1625	powerful antibacterial candidates with minor potential toxicity	1593:1655	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites could be impressively recommended as powerful antibacterial candidates with minor potential toxicity.
34146360	9	68	theme	combined	1522:1529	arg1	nanocomposites					1541:1554	the combined βG-Ag NPs nanocomposites	1518:1554	the combined βG-Ag NPs nanocomposites	1518:1554	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites could be impressively recommended as powerful antibacterial candidates with minor potential toxicity.
34146360	6	69	theme	βG-Ag	937:941	arg1	composite					947:955	βG-Ag NPs composite	937:955	βG-Ag NPs composite	937:955	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	6	69	theme	βG-Ag	937:941	arg1	βG					917:918	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite	901:955	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite	901:955	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	7	70	dep	Gram-	1216:1220	arg1	coli					1235:1238	Escherichia coli	1223:1238	Escherichia coli	1223:1238	The NPs (plain Ag NPs and composited βG-Ag NPs) exhibited potent comparable bactericidal actions, opposing Gram+ (Staphylococcus aureus) and Gram- (Escherichia coli, Salmonella Typhimurium, and Pseudomonas aeruginosa).
34146360	7	70	dep	Gram-	1216:1220	arg1	Typhimurium					1252:1262	Salmonella Typhimurium	1241:1262	Salmonella Typhimurium	1241:1262	The NPs (plain Ag NPs and composited βG-Ag NPs) exhibited potent comparable bactericidal actions, opposing Gram+ (Staphylococcus aureus) and Gram- (Escherichia coli, Salmonella Typhimurium, and Pseudomonas aeruginosa).
34146360	7	70	dep	Gram-	1216:1220	arg1	aeruginosa					1281:1290	Pseudomonas aeruginosa	1269:1290	Pseudomonas aeruginosa	1269:1290	The NPs (plain Ag NPs and composited βG-Ag NPs) exhibited potent comparable bactericidal actions, opposing Gram+ (Staphylococcus aureus) and Gram- (Escherichia coli, Salmonella Typhimurium, and Pseudomonas aeruginosa).
34146360	8	71	theme	bactericidal	1396:1407	arg1	actions					1409:1415	the powerful bactericidal actions	1383:1415	the powerful bactericidal actions of nanocomposite for destructing pathogens' cells	1383:1465	Scanning micrographs, of treated S. aureus and S. Typhimurium with βG-Ag NPs, elucidated the powerful bactericidal actions of nanocomposite for destructing pathogens' cells.
34146360	6	72	theme	58.65	1034:1038	arg1	diameters					1021:1029	mean diameters	1016:1029	mean diameters of 58.65, 6.72, and 63.88 nm, respectively	1016:1072	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	9	73	theme	NPs	1485:1487	arg1	biosynthesis					1489:1500	The inventive Ag NPs biosynthesis	1468:1500	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites	1468:1554	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites could be impressively recommended as powerful antibacterial candidates with minor potential toxicity.
34146360	9	73	theme	NPs	1485:1487	arg1	candidates					1616:1625	powerful antibacterial candidates	1593:1625	powerful antibacterial candidates with minor potential toxicity	1593:1655	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites could be impressively recommended as powerful antibacterial candidates with minor potential toxicity.
34146360	1	74	theme	elevated	236:243	arg1	biotoxicity					245:255	their elevated biotoxicity	230:255	their elevated biotoxicity	230:255	Nanometals (NM) frequently possess potent antimicrobial potentials to combat various pathogens, but their elevated biotoxicity limits their direct applications.
34146360	8	75	theme	aureus	1330:1335	arg1	micrographs					1303:1313	Scanning micrographs	1294:1313	Scanning micrographs	1294:1313	Scanning micrographs, of treated S. aureus and S. Typhimurium with βG-Ag NPs, elucidated the powerful bactericidal actions of nanocomposite for destructing pathogens' cells.
34146360	8	76	theme	powerful	1387:1394	arg1	actions					1409:1415	the powerful bactericidal actions	1383:1415	the powerful bactericidal actions of nanocomposite for destructing pathogens' cells	1383:1465	Scanning micrographs, of treated S. aureus and S. Typhimurium with βG-Ag NPs, elucidated the powerful bactericidal actions of nanocomposite for destructing pathogens' cells.
34146360	8	77	theme	Scanning	1294:1301	arg1	micrographs					1303:1313	Scanning micrographs	1294:1313	Scanning micrographs	1294:1313	Scanning micrographs, of treated S. aureus and S. Typhimurium with βG-Ag NPs, elucidated the powerful bactericidal actions of nanocomposite for destructing pathogens' cells.
34146360	2	78	with	biosynthesis	295:306	arg1	biopolymers					357:367	natural biopolymers	349:367	natural biopolymers	349:367	The biosynthesis of NM and their capping/conjugation with natural biopolymers can effectually enhance NM stability and diminish such toxicity.
34146360	5	79	theme	electron	800:807	arg1	assessments					723:733	The physicochemical assessments	703:733	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy)	703:819	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	5	79	theme	electron	800:807	arg1	microscopy					809:818	transmission electron microscopy	787:818	transmission electron microscopy	787:818	The physicochemical assessments (Fourier-transform infrared, X-ray diffraction, and transmission electron microscopy) validated NPs formation, interaction, and interior capping of Ag NPs in βG NPs.
34146360	7	80	dep	NPs	1079:1081	arg1	NPs					1118:1120	composited βG-Ag NPs	1101:1120	composited βG-Ag NPs	1101:1120	The NPs (plain Ag NPs and composited βG-Ag NPs) exhibited potent comparable bactericidal actions, opposing Gram+ (Staphylococcus aureus) and Gram- (Escherichia coli, Salmonella Typhimurium, and Pseudomonas aeruginosa).
34146360	7	80	dep	NPs	1079:1081	arg1	NPs					1093:1095	plain Ag NPs	1084:1095	plain Ag NPs	1084:1095	The NPs (plain Ag NPs and composited βG-Ag NPs) exhibited potent comparable bactericidal actions, opposing Gram+ (Staphylococcus aureus) and Gram- (Escherichia coli, Salmonella Typhimurium, and Pseudomonas aeruginosa).
34146360	6	81	theme	Ag	925:926	arg1	NPs					928:930	Ag NPs	925:930	Ag NPs	925:930	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	6	81	theme	Ag	925:926	arg1	βG					917:918	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite	901:955	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite	901:955	The synthesized βG NPs, Ag NPs, and βG-Ag NPs composite were negatively charged and had minute particle sizes with mean diameters of 58.65, 6.72, and 63.88 nm, respectively.
34146360	9	82	theme	potential	1638:1646	arg1	toxicity					1648:1655	minor potential toxicity	1632:1655	minor potential toxicity	1632:1655	The inventive Ag NPs biosynthesis with βG NPs and the combined βG-Ag NPs nanocomposites could be impressively recommended as powerful antibacterial candidates with minor potential toxicity.
34146360	3	83	theme	alkali/acid	545:555	arg1	protocol					564:571	alkali/acid facile protocol	545:571	alkali/acid facile protocol	545:571	Yeast β-glucan (βG), from Saccharomyces cerevisiae, was extracted and transformed to nanoparticles (NPs) using alkali/acid facile protocol.
34146360	4	84	theme	nanoparticles	646:658	arg1	biosynthesis					623:634	direct biosynthesis	616:634	direct biosynthesis of silver nanoparticles (Ag NPs) without extra chemical processes	616:700	The βG NPs were innovatively employed for direct biosynthesis of silver nanoparticles (Ag NPs) without extra chemical processes.
33765627	0	0	theme	luteus	97:102	arg1	fermentation					104:115	Micrococcus luteus fermentation	85:115	Micrococcus luteus fermentation	85:115	Electrospun nanofibers enhance trehalose synthesis by regulating gene expression for Micrococcus luteus fermentation.
33765627	9	1	theme	trehalose	1348:1356	arg1	production					1358:1367	sufficient trehalose production	1337:1367	sufficient trehalose production	1337:1367	Therefore, this research provided theoretical foundation for the metabolic regulation of sufficient trehalose production by immobilized cells.
33765627	9	2	theme	immobilized	1372:1382	arg1	cells					1384:1388	immobilized cells	1372:1388	immobilized cells	1372:1388	Therefore, this research provided theoretical foundation for the metabolic regulation of sufficient trehalose production by immobilized cells.
33765627	6	3	theme	species	868:874	arg1	level					882:886	the reactive oxygen species (ROS) level	848:886	the reactive oxygen species (ROS) level	848:886	Moreover, the reactive oxygen species (ROS) level was decreased to 12.8 % while the enzyme concentration was increased to 11.176 mg/mL.
33765627	7	4	theme	target	1076:1081	arg1	MtreY					1088:1092	target gene MtreY	1076:1092	target gene MtreY	1076:1092	Meanwhile, it was also found that PAN/TPU-P2 immobilization substantially increased the expression of target gene MtreY by 3.500 times.
33765627	8	5	theme	immobilized	1149:1159	arg1	cells					1161:1165	immobilized cells	1149:1165	immobilized cells	1149:1165	In other words, the mechanism by which immobilized cells increased trehalose yield was that PAN/TPU-P regulated gene expression of MtreY.
33765627	1	6	theme	/thermoplastic	167:180	arg1	nanofibers					209:218	mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers	133:218	mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers	133:218	In this study, mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers were prepared to immobilize Micrococcus luteus for enhancing the conversion of trehalose.
33765627	6	7	theme	oxygen	861:866	arg1	species					868:874	reactive oxygen species	852:874	the reactive oxygen species (ROS) level	848:886	Moreover, the reactive oxygen species (ROS) level was decreased to 12.8 % while the enzyme concentration was increased to 11.176 mg/mL.
33765627	6	7	theme	oxygen	861:866	arg1	ROS					877:879	ROS	877:879	ROS	877:879	Moreover, the reactive oxygen species (ROS) level was decreased to 12.8 % while the enzyme concentration was increased to 11.176 mg/mL.
33765627	6	8	theme	enzyme	922:927	arg1	concentration					929:941	the enzyme concentration	918:941	the enzyme concentration	918:941	Moreover, the reactive oxygen species (ROS) level was decreased to 12.8 % while the enzyme concentration was increased to 11.176 mg/mL.
33765627	6	9	theme	reactive	852:859	arg1	species					868:874	reactive oxygen species	852:874	the reactive oxygen species (ROS) level	848:886	Moreover, the reactive oxygen species (ROS) level was decreased to 12.8 % while the enzyme concentration was increased to 11.176 mg/mL.
33765627	6	9	theme	reactive	852:859	arg1	ROS					877:879	ROS	877:879	ROS	877:879	Moreover, the reactive oxygen species (ROS) level was decreased to 12.8 % while the enzyme concentration was increased to 11.176 mg/mL.
33765627	3	10	theme	PAN/TPU-P2	538:547	arg1	stability					525:533	stability	525:533	stability	525:533	The results of contact angle, Zeta potential and water holding ratio exhibited the good hydrophilicity and stability of PAN/TPU-P2.
33765627	3	10	theme	PAN/TPU-P2	538:547	arg1	hydrophilicity					506:519	hydrophilicity	506:519	hydrophilicity	506:519	The results of contact angle, Zeta potential and water holding ratio exhibited the good hydrophilicity and stability of PAN/TPU-P2.
33765627	3	11	theme	water	467:471	arg1	ratio					481:485	water holding ratio	467:485	water holding ratio	467:485	The results of contact angle, Zeta potential and water holding ratio exhibited the good hydrophilicity and stability of PAN/TPU-P2.
33765627	5	12	theme	trehalose	716:724	arg1	yield					726:730	the trehalose yield	712:730	the trehalose yield	712:730	It was the most noteworthy that the trehalose yield could reach 23.46 g/L, which was 71.62 % higher than that of the control in the multi-batch fermentation.
33765627	8	13	theme	other	1113:1117	arg1	words					1119:1123	other words	1113:1123	other words	1113:1123	In other words, the mechanism by which immobilized cells increased trehalose yield was that PAN/TPU-P regulated gene expression of MtreY.
33765627	1	14	theme	TPU	196:198	arg1	nanofibers					209:218	mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers	133:218	mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers	133:218	In this study, mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers were prepared to immobilize Micrococcus luteus for enhancing the conversion of trehalose.
33765627	1	15	theme	polyurethane	182:193	arg1	nanofibers					209:218	mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers	133:218	mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers	133:218	In this study, mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers were prepared to immobilize Micrococcus luteus for enhancing the conversion of trehalose.
33765627	7	16	theme	MtreY	1088:1092	arg1	expression					1062:1071	the expression	1058:1071	the expression of target gene MtreY	1058:1092	Meanwhile, it was also found that PAN/TPU-P2 immobilization substantially increased the expression of target gene MtreY by 3.500 times.
33765627	2	17	dep	surface	366:372	arg1	the					362:364	the	362:364	the	362:364	The images of SEM showed the cells were adsorbed on the surface and pores due to the unique pore structure.
33765627	3	18	theme	contact	433:439	arg1	angle					441:445	contact angle	433:445	contact angle	433:445	The results of contact angle, Zeta potential and water holding ratio exhibited the good hydrophilicity and stability of PAN/TPU-P2.
33765627	7	19	theme	PAN/TPU-P2	1008:1017	arg1	immobilization					1019:1032	PAN/TPU-P2 immobilization	1008:1032	PAN/TPU-P2 immobilization	1008:1032	Meanwhile, it was also found that PAN/TPU-P2 immobilization substantially increased the expression of target gene MtreY by 3.500 times.
33765627	2	20	theme	due	384:386	arg1	surface					366:372	surface	366:372	surface	366:372	The images of SEM showed the cells were adsorbed on the surface and pores due to the unique pore structure.
33765627	0	21	theme	Electrospun	0:10	arg1	nanofibers					12:21	Electrospun nanofibers	0:21	Electrospun nanofibers	0:21	Electrospun nanofibers enhance trehalose synthesis by regulating gene expression for Micrococcus luteus fermentation.
33765627	2	22	theme	SEM	324:326	arg1	images					314:319	The images	310:319	The images of SEM	310:326	The images of SEM showed the cells were adsorbed on the surface and pores due to the unique pore structure.
33765627	0	23	theme	trehalose	31:39	arg1	synthesis					41:49	trehalose synthesis	31:49	trehalose synthesis	31:49	Electrospun nanofibers enhance trehalose synthesis by regulating gene expression for Micrococcus luteus fermentation.
33765627	1	24	theme	trehalose	299:307	arg1	conversion					285:294	the conversion	281:294	the conversion of trehalose	281:307	In this study, mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers were prepared to immobilize Micrococcus luteus for enhancing the conversion of trehalose.
33765627	8	25	theme	MtreY	1241:1245	arg1	expression					1227:1236	gene expression	1222:1236	gene expression of MtreY	1222:1245	In other words, the mechanism by which immobilized cells increased trehalose yield was that PAN/TPU-P regulated gene expression of MtreY.
33765627	1	26	theme	blended	201:207	arg1	nanofibers					209:218	mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers	133:218	mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers	133:218	In this study, mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers were prepared to immobilize Micrococcus luteus for enhancing the conversion of trehalose.
33765627	3	27	dep	hydrophilicity	506:519	arg1	the					497:499	the	497:499	the	497:499	The results of contact angle, Zeta potential and water holding ratio exhibited the good hydrophilicity and stability of PAN/TPU-P2.
33765627	3	28	theme	potential	453:461	arg1	results					422:428	The results	418:428	The results of contact angle, Zeta potential and water holding ratio	418:485	The results of contact angle, Zeta potential and water holding ratio exhibited the good hydrophilicity and stability of PAN/TPU-P2.
33765627	3	29	theme	angle	441:445	arg1	results					422:428	The results	418:428	The results of contact angle, Zeta potential and water holding ratio	418:485	The results of contact angle, Zeta potential and water holding ratio exhibited the good hydrophilicity and stability of PAN/TPU-P2.
33765627	2	30	theme	pore	402:405	arg1	structure					407:415	the unique pore structure	391:415	the unique pore structure	391:415	The images of SEM showed the cells were adsorbed on the surface and pores due to the unique pore structure.
33765627	8	31	theme	trehalose	1177:1185	arg1	yield					1187:1191	trehalose yield	1177:1191	trehalose yield	1177:1191	In other words, the mechanism by which immobilized cells increased trehalose yield was that PAN/TPU-P regulated gene expression of MtreY.
33765627	8	32	theme	gene	1222:1225	arg1	expression					1227:1236	gene expression	1222:1236	gene expression of MtreY	1222:1245	In other words, the mechanism by which immobilized cells increased trehalose yield was that PAN/TPU-P regulated gene expression of MtreY.
33765627	5	33	from	control	797:803	arg1	fermentation					824:835	the multi-batch fermentation	808:835	the multi-batch fermentation	808:835	It was the most noteworthy that the trehalose yield could reach 23.46 g/L, which was 71.62 % higher than that of the control in the multi-batch fermentation.
33765627	2	34	theme	unique	395:400	arg1	structure					407:415	the unique pore structure	391:415	the unique pore structure	391:415	The images of SEM showed the cells were adsorbed on the surface and pores due to the unique pore structure.
33765627	0	35	theme	gene	65:68	arg1	expression					70:79	gene expression	65:79	gene expression for Micrococcus luteus fermentation	65:115	Electrospun nanofibers enhance trehalose synthesis by regulating gene expression for Micrococcus luteus fermentation.
33765627	9	36	theme	metabolic	1313:1321	arg1	regulation					1323:1332	the metabolic regulation	1309:1332	the metabolic regulation of sufficient trehalose production by immobilized cells	1309:1388	Therefore, this research provided theoretical foundation for the metabolic regulation of sufficient trehalose production by immobilized cells.
33765627	9	37	theme	theoretical	1282:1292	arg1	foundation					1294:1303	theoretical foundation	1282:1303	theoretical foundation for the metabolic regulation of sufficient trehalose production by immobilized cells	1282:1388	Therefore, this research provided theoretical foundation for the metabolic regulation of sufficient trehalose production by immobilized cells.
33765627	3	38	theme	holding	473:479	arg1	ratio					481:485	water holding ratio	467:485	water holding ratio	467:485	The results of contact angle, Zeta potential and water holding ratio exhibited the good hydrophilicity and stability of PAN/TPU-P2.
33765627	4	39	theme	biomass	585:591	arg1	efficiency					612:621	the biomass and immobilization efficiency	581:621	the biomass and immobilization efficiency	581:621	Besides, it was indicated that the biomass and immobilization efficiency were increased to 0.633 g/L and 0.153 g/g, respectively.
33765627	3	40	theme	ratio	481:485	arg1	results					422:428	The results	418:428	The results of contact angle, Zeta potential and water holding ratio	418:485	The results of contact angle, Zeta potential and water holding ratio exhibited the good hydrophilicity and stability of PAN/TPU-P2.
33765627	1	41	theme	mesoporous	133:142	arg1	nanofibers					209:218	mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers	133:218	mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers	133:218	In this study, mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers were prepared to immobilize Micrococcus luteus for enhancing the conversion of trehalose.
33765627	9	42	theme	sufficient	1337:1346	arg1	production					1358:1367	sufficient trehalose production	1337:1367	sufficient trehalose production	1337:1367	Therefore, this research provided theoretical foundation for the metabolic regulation of sufficient trehalose production by immobilized cells.
33765627	1	43	theme	polyacrylonitrile	144:160	arg1	nanofibers					209:218	mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers	133:218	mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers	133:218	In this study, mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers were prepared to immobilize Micrococcus luteus for enhancing the conversion of trehalose.
33765627	2	44	dep	showed	328:333	arg1	adsorbed					350:357	adsorbed	350:357	showed the cells were adsorbed on the surface and pores due to the unique pore structure	328:415	The images of SEM showed the cells were adsorbed on the surface and pores due to the unique pore structure.
33765627	3	45	theme	Zeta	448:451	arg1	potential					453:461	Zeta potential	448:461	Zeta potential	448:461	The results of contact angle, Zeta potential and water holding ratio exhibited the good hydrophilicity and stability of PAN/TPU-P2.
33765627	3	46	theme	good	501:504	arg1	hydrophilicity					506:519	hydrophilicity	506:519	hydrophilicity	506:519	The results of contact angle, Zeta potential and water holding ratio exhibited the good hydrophilicity and stability of PAN/TPU-P2.
33765627	5	47	theme	multi-batch	812:822	arg1	fermentation					824:835	the multi-batch fermentation	808:835	the multi-batch fermentation	808:835	It was the most noteworthy that the trehalose yield could reach 23.46 g/L, which was 71.62 % higher than that of the control in the multi-batch fermentation.
33765627	9	48	theme	production	1358:1367	arg1	regulation					1323:1332	the metabolic regulation	1309:1332	the metabolic regulation of sufficient trehalose production by immobilized cells	1309:1388	Therefore, this research provided theoretical foundation for the metabolic regulation of sufficient trehalose production by immobilized cells.
33765627	7	49	theme	gene	1083:1086	arg1	MtreY					1088:1092	target gene MtreY	1076:1092	target gene MtreY	1076:1092	Meanwhile, it was also found that PAN/TPU-P2 immobilization substantially increased the expression of target gene MtreY by 3.500 times.
33765627	1	50	theme	PAN	163:165	arg1	nanofibers					209:218	mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers	133:218	mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers	133:218	In this study, mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers were prepared to immobilize Micrococcus luteus for enhancing the conversion of trehalose.
33765627	4	51	theme	immobilization	597:610	arg1	efficiency					612:621	the biomass and immobilization efficiency	581:621	the biomass and immobilization efficiency	581:621	Besides, it was indicated that the biomass and immobilization efficiency were increased to 0.633 g/L and 0.153 g/g, respectively.
33317143	1	0	theme	valuable	133:140	arg1	N-glycome					118:126	The human serum N-glycome	102:126	The human serum N-glycome	102:126	The human serum N-glycome is a valuable source of biomarkers for malignant diseases, already utilized in multiple studies.
33317143	1	0	theme	valuable	133:140	arg1	source					142:147	a valuable source	131:147	a valuable source	131:147	The human serum N-glycome is a valuable source of biomarkers for malignant diseases, already utilized in multiple studies.
33317143	4	1	theme	relative	588:595	arg1	areas					602:606	The relative peak areas	584:606	The relative peak areas of 21 N-glycans	584:622	The relative peak areas of 21 N-glycans were evaluated from the acquired electropherograms using machine learning-based data analysis.
33317143	2	2	theme	tumor	326:330	arg1	resection					332:340	surgical lung tumor resection	312:340	surgical lung tumor resection	312:340	In this paper, the N-glycosylation changes in human serum proteins were analyzed after surgical lung tumor resection.
33317143	0	3	theme	Tumor	87:91	arg1	Surgery					93:99	Lung Tumor Surgery	82:99	Lung Tumor Surgery	82:99	Machine Learning Based Analysis of Human Serum N-glycome Alterations to Follow up Lung Tumor Surgery.
33317143	6	4	theme	data	831:834	arg1	analysis					836:843	the data analysis	827:843	the data analysis	827:843	For the data analysis, both discrete (e.g., smoker or not) and continuous (e.g., age of the patient) clinical parameters were compared against the alterations in these 21 N-linked carbohydrate structures.
33317143	5	5	theme	evaluation	811:820	arg1	course					801:806	the course	797:806	the course of evaluation	797:820	Individual glycans as well as their subclasses were taken into account during the course of evaluation.
33317143	3	6	theme	fluorescent	561:571	arg1	detection					573:581	laser-induced fluorescent detection	547:581	laser-induced fluorescent detection	547:581	Seventeen lung cancer patients were involved in this study and the N-glycosylation pattern of their serum samples was analyzed before and after the surgery using capillary electrophoresis separation with laser-induced fluorescent detection.
33317143	6	7	dep	age	904:906	arg1	e.g.					898:901	e.g.	898:901	e.g.	898:901	For the data analysis, both discrete (e.g., smoker or not) and continuous (e.g., age of the patient) clinical parameters were compared against the alterations in these 21 N-linked carbohydrate structures.
33317143	0	8	theme	Lung	82:85	arg1	Surgery					93:99	Lung Tumor Surgery	82:99	Lung Tumor Surgery	82:99	Machine Learning Based Analysis of Human Serum N-glycome Alterations to Follow up Lung Tumor Surgery.
33317143	6	9	theme	continuous	886:895	arg1	parameters					933:942	both discrete (e.g., smoker or not) and continuous (e.g., age of the patient) clinical parameters	846:942	both discrete (e.g., smoker or not) and continuous (e.g., age of the patient) clinical parameters	846:942	For the data analysis, both discrete (e.g., smoker or not) and continuous (e.g., age of the patient) clinical parameters were compared against the alterations in these 21 N-linked carbohydrate structures.
33317143	6	10	link	N-linked	994:1001	arg1	structures					1016:1025	these 21 N-linked carbohydrate structures	985:1025	these 21 N-linked carbohydrate structures	985:1025	For the data analysis, both discrete (e.g., smoker or not) and continuous (e.g., age of the patient) clinical parameters were compared against the alterations in these 21 N-linked carbohydrate structures.
33317143	4	11	theme	peak	597:600	arg1	areas					602:606	The relative peak areas	584:606	The relative peak areas of 21 N-glycans	584:622	The relative peak areas of 21 N-glycans were evaluated from the acquired electropherograms using machine learning-based data analysis.
33317143	7	12	theme	lung	1146:1149	arg1	resection					1166:1174	lung tumor surgical resection	1146:1174	lung tumor surgical resection	1146:1174	The classification tree analysis resulted in a panel of N-glycans, which could be used to follow up on the effects of lung tumor surgical resection.
33317143	2	13	theme	N-glycosylation	244:258	arg1	changes					260:266	the N-glycosylation changes	240:266	the N-glycosylation changes in human serum proteins	240:290	In this paper, the N-glycosylation changes in human serum proteins were analyzed after surgical lung tumor resection.
33317143	3	14	theme	N-glycosylation	410:424	arg1	pattern					426:432	the N-glycosylation pattern	406:432	the N-glycosylation pattern of their serum samples	406:455	Seventeen lung cancer patients were involved in this study and the N-glycosylation pattern of their serum samples was analyzed before and after the surgery using capillary electrophoresis separation with laser-induced fluorescent detection.
33317143	7	15	theme	tumor	1151:1155	arg1	resection					1166:1174	lung tumor surgical resection	1146:1174	lung tumor surgical resection	1146:1174	The classification tree analysis resulted in a panel of N-glycans, which could be used to follow up on the effects of lung tumor surgical resection.
33317143	7	16	theme	N-glycans	1084:1092	arg1	panel					1075:1079	a panel	1073:1079	a panel	1073:1079	The classification tree analysis resulted in a panel of N-glycans, which could be used to follow up on the effects of lung tumor surgical resection.
33317143	7	17	theme	surgical	1157:1164	arg1	resection					1166:1174	lung tumor surgical resection	1146:1174	lung tumor surgical resection	1146:1174	The classification tree analysis resulted in a panel of N-glycans, which could be used to follow up on the effects of lung tumor surgical resection.
33317143	3	18	theme	electrophoresis	515:529	arg1	separation					531:540	capillary electrophoresis separation	505:540	capillary electrophoresis separation with laser-induced fluorescent detection	505:581	Seventeen lung cancer patients were involved in this study and the N-glycosylation pattern of their serum samples was analyzed before and after the surgery using capillary electrophoresis separation with laser-induced fluorescent detection.
33317143	1	19	theme	biomarkers	152:161	arg1	N-glycome					118:126	The human serum N-glycome	102:126	The human serum N-glycome	102:126	The human serum N-glycome is a valuable source of biomarkers for malignant diseases, already utilized in multiple studies.
33317143	1	19	theme	biomarkers	152:161	arg1	source					142:147	a valuable source	131:147	a valuable source	131:147	The human serum N-glycome is a valuable source of biomarkers for malignant diseases, already utilized in multiple studies.
33317143	6	20	theme	clinical	924:931	arg1	parameters					933:942	both discrete (e.g., smoker or not) and continuous (e.g., age of the patient) clinical parameters	846:942	both discrete (e.g., smoker or not) and continuous (e.g., age of the patient) clinical parameters	846:942	For the data analysis, both discrete (e.g., smoker or not) and continuous (e.g., age of the patient) clinical parameters were compared against the alterations in these 21 N-linked carbohydrate structures.
33317143	6	21	dep	discrete	851:858	arg1	not					877:879	not	877:879	not	877:879	For the data analysis, both discrete (e.g., smoker or not) and continuous (e.g., age of the patient) clinical parameters were compared against the alterations in these 21 N-linked carbohydrate structures.
33317143	6	21	dep	discrete	851:858	arg1	smoker					867:872	smoker	867:872	smoker	867:872	For the data analysis, both discrete (e.g., smoker or not) and continuous (e.g., age of the patient) clinical parameters were compared against the alterations in these 21 N-linked carbohydrate structures.
33317143	6	21	dep	discrete	851:858	arg1	age					904:906	age	904:906	age	904:906	For the data analysis, both discrete (e.g., smoker or not) and continuous (e.g., age of the patient) clinical parameters were compared against the alterations in these 21 N-linked carbohydrate structures.
33317143	7	22	theme	classification	1032:1045	arg1	analysis					1052:1059	The classification tree analysis	1028:1059	The classification tree analysis	1028:1059	The classification tree analysis resulted in a panel of N-glycans, which could be used to follow up on the effects of lung tumor surgical resection.
33317143	4	23	theme	learning-based	689:702	arg1	analysis					709:716	machine learning-based data analysis	681:716	machine learning-based data analysis	681:716	The relative peak areas of 21 N-glycans were evaluated from the acquired electropherograms using machine learning-based data analysis.
33317143	0	24	theme	Machine	0:6	arg1	Learning					8:15	Machine Learning	0:15	Machine Learning	0:15	Machine Learning Based Analysis of Human Serum N-glycome Alterations to Follow up Lung Tumor Surgery.
33317143	6	25	from	alterations	970:980	arg1	structures					1016:1025	these 21 N-linked carbohydrate structures	985:1025	these 21 N-linked carbohydrate structures	985:1025	For the data analysis, both discrete (e.g., smoker or not) and continuous (e.g., age of the patient) clinical parameters were compared against the alterations in these 21 N-linked carbohydrate structures.
33317143	1	26	theme	malignant	167:175	arg1	diseases					177:184	malignant diseases	167:184	malignant diseases	167:184	The human serum N-glycome is a valuable source of biomarkers for malignant diseases, already utilized in multiple studies.
33317143	3	27	theme	lung	353:356	arg1	patients					365:372	Seventeen lung cancer patients	343:372	Seventeen lung cancer patients	343:372	Seventeen lung cancer patients were involved in this study and the N-glycosylation pattern of their serum samples was analyzed before and after the surgery using capillary electrophoresis separation with laser-induced fluorescent detection.
33317143	2	28	theme	serum	277:281	arg1	proteins					283:290	human serum proteins	271:290	human serum proteins	271:290	In this paper, the N-glycosylation changes in human serum proteins were analyzed after surgical lung tumor resection.
33317143	3	29	theme	serum	443:447	arg1	samples					449:455	their serum samples	437:455	their serum samples	437:455	Seventeen lung cancer patients were involved in this study and the N-glycosylation pattern of their serum samples was analyzed before and after the surgery using capillary electrophoresis separation with laser-induced fluorescent detection.
33317143	3	30	theme	cancer	358:363	arg1	patients					365:372	Seventeen lung cancer patients	343:372	Seventeen lung cancer patients	343:372	Seventeen lung cancer patients were involved in this study and the N-glycosylation pattern of their serum samples was analyzed before and after the surgery using capillary electrophoresis separation with laser-induced fluorescent detection.
33317143	3	31	theme	capillary	505:513	arg1	electrophoresis					515:529	capillary electrophoresis	505:529	capillary electrophoresis separation with laser-induced fluorescent detection	505:581	Seventeen lung cancer patients were involved in this study and the N-glycosylation pattern of their serum samples was analyzed before and after the surgery using capillary electrophoresis separation with laser-induced fluorescent detection.
33317143	2	32	theme	human	271:275	arg1	proteins					283:290	human serum proteins	271:290	human serum proteins	271:290	In this paper, the N-glycosylation changes in human serum proteins were analyzed after surgical lung tumor resection.
33317143	3	33	theme	samples	449:455	arg1	pattern					426:432	the N-glycosylation pattern	406:432	the N-glycosylation pattern of their serum samples	406:455	Seventeen lung cancer patients were involved in this study and the N-glycosylation pattern of their serum samples was analyzed before and after the surgery using capillary electrophoresis separation with laser-induced fluorescent detection.
33317143	0	34	theme	Human	35:39	arg1	Alterations					57:67	Human Serum N-glycome Alterations	35:67	Human Serum N-glycome Alterations	35:67	Machine Learning Based Analysis of Human Serum N-glycome Alterations to Follow up Lung Tumor Surgery.
33317143	2	35	theme	surgical	312:319	arg1	resection					332:340	surgical lung tumor resection	312:340	surgical lung tumor resection	312:340	In this paper, the N-glycosylation changes in human serum proteins were analyzed after surgical lung tumor resection.
33317143	4	36	theme	acquired	648:655	arg1	electropherograms					657:673	the acquired electropherograms	644:673	the acquired electropherograms using machine learning-based data analysis	644:716	The relative peak areas of 21 N-glycans were evaluated from the acquired electropherograms using machine learning-based data analysis.
33317143	4	37	theme	machine	681:687	arg1	analysis					709:716	machine learning-based data analysis	681:716	machine learning-based data analysis	681:716	The relative peak areas of 21 N-glycans were evaluated from the acquired electropherograms using machine learning-based data analysis.
33317143	3	38	with	separation	531:540	arg1	detection					573:581	laser-induced fluorescent detection	547:581	laser-induced fluorescent detection	547:581	Seventeen lung cancer patients were involved in this study and the N-glycosylation pattern of their serum samples was analyzed before and after the surgery using capillary electrophoresis separation with laser-induced fluorescent detection.
33317143	0	39	theme	N-glycome	47:55	arg1	Alterations					57:67	Human Serum N-glycome Alterations	35:67	Human Serum N-glycome Alterations	35:67	Machine Learning Based Analysis of Human Serum N-glycome Alterations to Follow up Lung Tumor Surgery.
33317143	6	40	theme	patient	915:921	arg1	age					904:906	age	904:906	age	904:906	For the data analysis, both discrete (e.g., smoker or not) and continuous (e.g., age of the patient) clinical parameters were compared against the alterations in these 21 N-linked carbohydrate structures.
33317143	2	41	theme	lung	321:324	arg1	resection					332:340	surgical lung tumor resection	312:340	surgical lung tumor resection	312:340	In this paper, the N-glycosylation changes in human serum proteins were analyzed after surgical lung tumor resection.
33317143	0	42	theme	Serum	41:45	arg1	Alterations					57:67	Human Serum N-glycome Alterations	35:67	Human Serum N-glycome Alterations	35:67	Machine Learning Based Analysis of Human Serum N-glycome Alterations to Follow up Lung Tumor Surgery.
33317143	7	43	used	used	1110:1113	arg2	panel					1075:1079	a panel	1073:1079	a panel	1073:1079	The classification tree analysis resulted in a panel of N-glycans, which could be used to follow up on the effects of lung tumor surgical resection.
33317143	7	44	theme	resection	1166:1174	arg1	effects					1135:1141	the effects	1131:1141	the effects of lung tumor surgical resection	1131:1174	The classification tree analysis resulted in a panel of N-glycans, which could be used to follow up on the effects of lung tumor surgical resection.
33317143	1	45	theme	human	106:110	arg1	N-glycome					118:126	The human serum N-glycome	102:126	The human serum N-glycome	102:126	The human serum N-glycome is a valuable source of biomarkers for malignant diseases, already utilized in multiple studies.
33317143	1	45	theme	human	106:110	arg1	source					142:147	a valuable source	131:147	a valuable source	131:147	The human serum N-glycome is a valuable source of biomarkers for malignant diseases, already utilized in multiple studies.
33317143	6	46	dep	smoker	867:872	arg1	e.g.					861:864	e.g.	861:864	e.g.	861:864	For the data analysis, both discrete (e.g., smoker or not) and continuous (e.g., age of the patient) clinical parameters were compared against the alterations in these 21 N-linked carbohydrate structures.
33317143	6	47	theme	discrete	851:858	arg1	parameters					933:942	both discrete (e.g., smoker or not) and continuous (e.g., age of the patient) clinical parameters	846:942	both discrete (e.g., smoker or not) and continuous (e.g., age of the patient) clinical parameters	846:942	For the data analysis, both discrete (e.g., smoker or not) and continuous (e.g., age of the patient) clinical parameters were compared against the alterations in these 21 N-linked carbohydrate structures.
33317143	6	48	theme	N-linked	994:1001	arg1	structures					1016:1025	these 21 N-linked carbohydrate structures	985:1025	these 21 N-linked carbohydrate structures	985:1025	For the data analysis, both discrete (e.g., smoker or not) and continuous (e.g., age of the patient) clinical parameters were compared against the alterations in these 21 N-linked carbohydrate structures.
33317143	1	49	theme	serum	112:116	arg1	N-glycome					118:126	The human serum N-glycome	102:126	The human serum N-glycome	102:126	The human serum N-glycome is a valuable source of biomarkers for malignant diseases, already utilized in multiple studies.
33317143	1	49	theme	serum	112:116	arg1	source					142:147	a valuable source	131:147	a valuable source	131:147	The human serum N-glycome is a valuable source of biomarkers for malignant diseases, already utilized in multiple studies.
33317143	3	50	theme	laser-induced	547:559	arg1	detection					573:581	laser-induced fluorescent detection	547:581	laser-induced fluorescent detection	547:581	Seventeen lung cancer patients were involved in this study and the N-glycosylation pattern of their serum samples was analyzed before and after the surgery using capillary electrophoresis separation with laser-induced fluorescent detection.
33317143	6	51	theme	carbohydrate	1003:1014	arg1	structures					1016:1025	these 21 N-linked carbohydrate structures	985:1025	these 21 N-linked carbohydrate structures	985:1025	For the data analysis, both discrete (e.g., smoker or not) and continuous (e.g., age of the patient) clinical parameters were compared against the alterations in these 21 N-linked carbohydrate structures.
33317143	5	52	theme	Individual	719:728	arg1	glycans					730:736	Individual glycans	719:736	Individual glycans as well as their subclasses	719:764	Individual glycans as well as their subclasses were taken into account during the course of evaluation.
33317143	3	53	gly	N-glycosylation	410:424	arg1	samples					449:455	their serum samples	437:455	their serum samples	437:455	Seventeen lung cancer patients were involved in this study and the N-glycosylation pattern of their serum samples was analyzed before and after the surgery using capillary electrophoresis separation with laser-induced fluorescent detection.
33317143	1	54	theme	multiple	207:214	arg1	studies					216:222	multiple studies	207:222	multiple studies	207:222	The human serum N-glycome is a valuable source of biomarkers for malignant diseases, already utilized in multiple studies.
33317143	2	55	from	changes	260:266	arg1	proteins					283:290	human serum proteins	271:290	human serum proteins	271:290	In this paper, the N-glycosylation changes in human serum proteins were analyzed after surgical lung tumor resection.
33317143	0	56	theme	Alterations	57:67	arg1	Analysis					23:30	Analysis	23:30	Analysis of Human Serum N-glycome Alterations	23:67	Machine Learning Based Analysis of Human Serum N-glycome Alterations to Follow up Lung Tumor Surgery.
33317143	4	57	theme	data	704:707	arg1	analysis					709:716	machine learning-based data analysis	681:716	machine learning-based data analysis	681:716	The relative peak areas of 21 N-glycans were evaluated from the acquired electropherograms using machine learning-based data analysis.
33317143	4	58	theme	N-glycans	614:622	arg1	areas					602:606	The relative peak areas	584:606	The relative peak areas of 21 N-glycans	584:622	The relative peak areas of 21 N-glycans were evaluated from the acquired electropherograms using machine learning-based data analysis.
32681312	12	0	theme	polar	1256:1260	arg1	microorganism					1280:1292	the polar lichen-associated microorganism	1252:1292	the polar lichen-associated microorganism	1252:1292	However, the polar lichen-associated microorganism was found to have a high percentage of hemicellulose degradation genes, and less starch and laminarin degradation.
32681312	13	1	theme	genes	1557:1561	arg1	number					1514:1519	a lower number	1506:1519	a lower number of starch and laminarin degradation genes	1506:1561	The Hymenobacter species with higher number of hemicellulose degradation genes was found to have a lower number of starch and laminarin degradation genes and vice versa, highlighting the differences in polysaccharide utilization among the species.
32681312	3	2	theme	family	232:237	arg1	Hymenobacteraceae					239:255	the family Hymenobacteraceae	228:255	the family Hymenobacteraceae	228:255	The genus Hymenobacter is classified in the family Hymenobacteraceae under the phylum Bacteroidetes.
32681312	5	3	theme	novel	534:538	arg1	microorganisms					540:553	novel microorganisms	534:553	novel microorganisms	534:553	The polar regions have attracted intense research interest for the discovery of novel microorganisms and their functions.
32681312	9	4	theme	polar	949:953	arg1	species					973:979	the two polar lichen-associated species	941:979	the two polar lichen-associated species	941:979	The study was conducted relying on the AZCL screening data for the two polar lichen-associated species.
32681312	10	5	theme	biosample	1114:1122	arg1	source					1124:1129	biosample source	1114:1129	biosample source	1114:1129	While comparing with eight other complete genomes, differences in polysaccharide preferences based on the isolation environment and biosample source were discovered.
32681312	4	6	theme	polar	390:394	arg1	regions					445:451	the Arctic and Antarctic regions	420:451	the Arctic and Antarctic regions	420:451	They have been isolated from diverse environments, such as air, soil, and lichen, along with extreme polar environments, including the Arctic and Antarctic regions.
32681312	4	6	theme	polar	390:394	arg1	environments					396:407	extreme polar environments	382:407	extreme polar environments	382:407	They have been isolated from diverse environments, such as air, soil, and lichen, along with extreme polar environments, including the Arctic and Antarctic regions.
32681312	9	7	theme	lichen-associated	955:971	arg1	species					973:979	the two polar lichen-associated species	941:979	the two polar lichen-associated species	941:979	The study was conducted relying on the AZCL screening data for the two polar lichen-associated species.
32681312	4	8	theme	extreme	382:388	arg1	regions					445:451	the Arctic and Antarctic regions	420:451	the Arctic and Antarctic regions	420:451	They have been isolated from diverse environments, such as air, soil, and lichen, along with extreme polar environments, including the Arctic and Antarctic regions.
32681312	4	8	theme	extreme	382:388	arg1	environments					396:407	extreme polar environments	382:407	extreme polar environments	382:407	They have been isolated from diverse environments, such as air, soil, and lichen, along with extreme polar environments, including the Arctic and Antarctic regions.
32681312	11	9	theme	genes	1236:1240	arg1	percentage					1186:1195	almost similar percentage	1171:1195	almost similar percentage of cellulose synthesis and degradation genes	1171:1240	All the species showed almost similar percentage of cellulose synthesis and degradation genes.
32681312	13	10	theme	degradation	1470:1480	arg1	genes					1482:1486	hemicellulose degradation genes	1456:1486	hemicellulose degradation genes	1456:1486	The Hymenobacter species with higher number of hemicellulose degradation genes was found to have a lower number of starch and laminarin degradation genes and vice versa, highlighting the differences in polysaccharide utilization among the species.
32681312	8	11	theme	genus	840:844	arg1	genome					821:826	the complete genome	808:826	the complete genome of the same genus available in the NCBI database	808:875	PAMC 26628 was performed, along with its comparison with the complete genome of the same genus available in the NCBI database.
32681312	1	12	theme	PAMC	70:73	arg1	26554					75:79	PAMC 26554	70:79	PAMC 26554	70:79	PAMC 26554 and Hymenobacter sp.
32681312	5	13	theme	intense	487:493	arg1	interest					504:511	intense research interest	487:511	intense research interest	487:511	The polar regions have attracted intense research interest for the discovery of novel microorganisms and their functions.
32681312	8	14	theme	same	835:838	arg1	genus					840:844	the same genus	831:844	the same genus available in the NCBI database	831:875	PAMC 26628 was performed, along with its comparison with the complete genome of the same genus available in the NCBI database.
32681312	12	15	theme	genes	1359:1363	arg1	laminarin					1386:1394	laminarin	1386:1394	laminarin	1386:1394	However, the polar lichen-associated microorganism was found to have a high percentage of hemicellulose degradation genes, and less starch and laminarin degradation.
32681312	12	15	theme	genes	1359:1363	arg1	percentage					1319:1328	a high percentage	1312:1328	a high percentage of hemicellulose degradation genes	1312:1363	However, the polar lichen-associated microorganism was found to have a high percentage of hemicellulose degradation genes, and less starch and laminarin degradation.
32681312	12	15	theme	genes	1359:1363	arg1	starch					1375:1380	starch	1375:1380	starch	1375:1380	However, the polar lichen-associated microorganism was found to have a high percentage of hemicellulose degradation genes, and less starch and laminarin degradation.
32681312	2	16	theme	Ability	162:168	arg1	Presence					125:132	the Presence	121:132	the Presence of Polysaccharide-Degrading Ability Based on Habitat	121:185	PAMC 26628 Reveals the Presence of Polysaccharide-Degrading Ability Based on Habitat.
32681312	3	17	theme	phylum	267:272	arg1	Bacteroidetes					274:286	the phylum Bacteroidetes	263:286	the phylum Bacteroidetes	263:286	The genus Hymenobacter is classified in the family Hymenobacteraceae under the phylum Bacteroidetes.
32681312	5	18	theme	research	495:502	arg1	interest					504:511	intense research interest	487:511	intense research interest	487:511	The polar regions have attracted intense research interest for the discovery of novel microorganisms and their functions.
32681312	2	19	theme	Polysaccharide-Degrading	137:160	arg1	Ability					162:168	Polysaccharide-Degrading Ability	137:168	Polysaccharide-Degrading Ability	137:168	PAMC 26628 Reveals the Presence of Polysaccharide-Degrading Ability Based on Habitat.
32681312	13	20	contain	have	1501:1504	arg2	number					1514:1519	a lower number	1506:1519	a lower number of starch and laminarin degradation genes	1506:1561	The Hymenobacter species with higher number of hemicellulose degradation genes was found to have a lower number of starch and laminarin degradation genes and vice versa, highlighting the differences in polysaccharide utilization among the species.
32681312	13	20	contain	have	1501:1504	arg2	versa					1572:1576	versa	1572:1576	versa	1572:1576	The Hymenobacter species with higher number of hemicellulose degradation genes was found to have a lower number of starch and laminarin degradation genes and vice versa, highlighting the differences in polysaccharide utilization among the species.
32681312	13	20	contain	have	1501:1504	arg1	species					1426:1432	The Hymenobacter species	1409:1432	The Hymenobacter species with higher number of hemicellulose degradation genes	1409:1486	The Hymenobacter species with higher number of hemicellulose degradation genes was found to have a lower number of starch and laminarin degradation genes and vice versa, highlighting the differences in polysaccharide utilization among the species.
32681312	6	21	theme	carbohydrate-active	627:645	arg1	enzyme					647:652	the polysaccharide utilization-related carbohydrate-active enzyme	588:652	the polysaccharide utilization-related carbohydrate-active enzyme	588:652	Analysis of the polysaccharide utilization-related carbohydrate-active enzyme among the two lichen-associated polar organisms Hymenobacter sp.
32681312	11	22	theme	cellulose	1200:1208	arg1	synthesis					1210:1218	cellulose synthesis	1200:1218	cellulose synthesis	1200:1218	All the species showed almost similar percentage of cellulose synthesis and degradation genes.
32681312	0	23	theme	Genomic	12:18	arg1	Study					20:24	Comparative Genomic Study	0:24	Comparative Genomic Study of Polar Lichen-Associated Hymenobacter sp	0:67	Comparative Genomic Study of Polar Lichen-Associated Hymenobacter sp.
32681312	1	24	theme	Hymenobacter	85:96	arg1	sp					98:99	Hymenobacter sp	85:99	Hymenobacter sp	85:99	PAMC 26554 and Hymenobacter sp.
32681312	4	25	theme	diverse	318:324	arg1	soil					353:356	soil	353:356	soil	353:356	They have been isolated from diverse environments, such as air, soil, and lichen, along with extreme polar environments, including the Arctic and Antarctic regions.
32681312	4	25	theme	diverse	318:324	arg1	lichen					363:368	lichen	363:368	lichen	363:368	They have been isolated from diverse environments, such as air, soil, and lichen, along with extreme polar environments, including the Arctic and Antarctic regions.
32681312	4	25	theme	diverse	318:324	arg1	air					348:350	air	348:350	air	348:350	They have been isolated from diverse environments, such as air, soil, and lichen, along with extreme polar environments, including the Arctic and Antarctic regions.
32681312	4	25	theme	diverse	318:324	arg1	environments					326:337	diverse environments	318:337	diverse environments	318:337	They have been isolated from diverse environments, such as air, soil, and lichen, along with extreme polar environments, including the Arctic and Antarctic regions.
32681312	0	26	theme	Comparative	0:10	arg1	Study					20:24	Comparative Genomic Study	0:24	Comparative Genomic Study of Polar Lichen-Associated Hymenobacter sp	0:67	Comparative Genomic Study of Polar Lichen-Associated Hymenobacter sp.
32681312	13	27	theme	hemicellulose	1456:1468	arg1	genes					1482:1486	hemicellulose degradation genes	1456:1486	hemicellulose degradation genes	1456:1486	The Hymenobacter species with higher number of hemicellulose degradation genes was found to have a lower number of starch and laminarin degradation genes and vice versa, highlighting the differences in polysaccharide utilization among the species.
32681312	7	28	theme	PAMC	719:722	arg1	26554					724:728	PAMC 26554	719:728	PAMC 26554	719:728	PAMC 26554 and Hymenobacter sp.
32681312	3	29	theme	genus	192:196	arg1	Hymenobacter					198:209	The genus Hymenobacter	188:209	The genus Hymenobacter	188:209	The genus Hymenobacter is classified in the family Hymenobacteraceae under the phylum Bacteroidetes.
32681312	5	30	theme	polar	458:462	arg1	regions					464:470	The polar regions	454:470	The polar regions	454:470	The polar regions have attracted intense research interest for the discovery of novel microorganisms and their functions.
32681312	8	31	theme	available	846:854	arg1	genus					840:844	the same genus	831:844	the same genus available in the NCBI database	831:875	PAMC 26628 was performed, along with its comparison with the complete genome of the same genus available in the NCBI database.
32681312	6	32	theme	utilization-related	607:625	arg1	enzyme					647:652	the polysaccharide utilization-related carbohydrate-active enzyme	588:652	the polysaccharide utilization-related carbohydrate-active enzyme	588:652	Analysis of the polysaccharide utilization-related carbohydrate-active enzyme among the two lichen-associated polar organisms Hymenobacter sp.
32681312	13	33	theme	polysaccharide	1611:1624	arg1	utilization					1626:1636	polysaccharide utilization	1611:1636	polysaccharide utilization	1611:1636	The Hymenobacter species with higher number of hemicellulose degradation genes was found to have a lower number of starch and laminarin degradation genes and vice versa, highlighting the differences in polysaccharide utilization among the species.
32681312	4	34	attach	isolated	304:311	arg1	soil					353:356	soil	353:356	soil	353:356	They have been isolated from diverse environments, such as air, soil, and lichen, along with extreme polar environments, including the Arctic and Antarctic regions.
32681312	4	34	attach	isolated	304:311	arg1	lichen					363:368	lichen	363:368	lichen	363:368	They have been isolated from diverse environments, such as air, soil, and lichen, along with extreme polar environments, including the Arctic and Antarctic regions.
32681312	4	34	attach	isolated	304:311	arg1	air					348:350	air	348:350	air	348:350	They have been isolated from diverse environments, such as air, soil, and lichen, along with extreme polar environments, including the Arctic and Antarctic regions.
32681312	4	34	attach	isolated	304:311	arg2	They					289:292	They	289:292	They	289:292	They have been isolated from diverse environments, such as air, soil, and lichen, along with extreme polar environments, including the Arctic and Antarctic regions.
32681312	4	34	attach	isolated	304:311	arg1	environments					326:337	diverse environments	318:337	diverse environments	318:337	They have been isolated from diverse environments, such as air, soil, and lichen, along with extreme polar environments, including the Arctic and Antarctic regions.
32681312	11	35	theme	degradation	1224:1234	arg1	genes					1236:1240	degradation genes	1224:1240	degradation genes	1224:1240	All the species showed almost similar percentage of cellulose synthesis and degradation genes.
32681312	6	36	theme	polysaccharide	592:605	arg1	enzyme					647:652	the polysaccharide utilization-related carbohydrate-active enzyme	588:652	the polysaccharide utilization-related carbohydrate-active enzyme	588:652	Analysis of the polysaccharide utilization-related carbohydrate-active enzyme among the two lichen-associated polar organisms Hymenobacter sp.
32681312	13	37	theme	genes	1482:1486	arg1	number					1446:1451	higher number	1439:1451	higher number of hemicellulose degradation genes	1439:1486	The Hymenobacter species with higher number of hemicellulose degradation genes was found to have a lower number of starch and laminarin degradation genes and vice versa, highlighting the differences in polysaccharide utilization among the species.
32681312	8	38	from	database	868:875	arg1	available					846:854	available	846:854	available	846:854	PAMC 26628 was performed, along with its comparison with the complete genome of the same genus available in the NCBI database.
32681312	10	39	from	differences	1033:1043	arg1	preferences					1063:1073	polysaccharide preferences	1048:1073	polysaccharide preferences based on the isolation environment and biosample source	1048:1129	While comparing with eight other complete genomes, differences in polysaccharide preferences based on the isolation environment and biosample source were discovered.
32681312	13	40	theme	laminarin	1535:1543	arg1	degradation					1545:1555	laminarin degradation	1535:1555	laminarin degradation	1535:1555	The Hymenobacter species with higher number of hemicellulose degradation genes was found to have a lower number of starch and laminarin degradation genes and vice versa, highlighting the differences in polysaccharide utilization among the species.
32681312	0	41	theme	Lichen-Associated	35:51	arg1	sp					66:67	Polar Lichen-Associated Hymenobacter sp	29:67	Polar Lichen-Associated Hymenobacter sp	29:67	Comparative Genomic Study of Polar Lichen-Associated Hymenobacter sp.
32681312	4	42	theme	Antarctic	435:443	arg1	regions					445:451	the Arctic and Antarctic regions	420:451	the Arctic and Antarctic regions	420:451	They have been isolated from diverse environments, such as air, soil, and lichen, along with extreme polar environments, including the Arctic and Antarctic regions.
32681312	10	43	theme	other	1009:1013	arg1	genomes					1024:1030	eight other complete genomes	1003:1030	eight other complete genomes	1003:1030	While comparing with eight other complete genomes, differences in polysaccharide preferences based on the isolation environment and biosample source were discovered.
32681312	12	44	dep	starch	1375:1380	arg1	degradation					1396:1406	degradation	1396:1406	degradation	1396:1406	However, the polar lichen-associated microorganism was found to have a high percentage of hemicellulose degradation genes, and less starch and laminarin degradation.
32681312	12	45	theme	degradation	1347:1357	arg1	genes					1359:1363	hemicellulose degradation genes	1333:1363	hemicellulose degradation genes	1333:1363	However, the polar lichen-associated microorganism was found to have a high percentage of hemicellulose degradation genes, and less starch and laminarin degradation.
32681312	0	46	theme	Polar	29:33	arg1	sp					66:67	Polar Lichen-Associated Hymenobacter sp	29:67	Polar Lichen-Associated Hymenobacter sp	29:67	Comparative Genomic Study of Polar Lichen-Associated Hymenobacter sp.
32681312	2	47	theme	PAMC	102:105	arg1	26628					107:111	PAMC 26628	102:111	PAMC 26628	102:111	PAMC 26628 Reveals the Presence of Polysaccharide-Degrading Ability Based on Habitat.
32681312	7	48	theme	Hymenobacter	734:745	arg1	sp					747:748	Hymenobacter sp	734:748	Hymenobacter sp	734:748	PAMC 26554 and Hymenobacter sp.
32681312	11	49	theme	similar	1178:1184	arg1	percentage					1186:1195	almost similar percentage	1171:1195	almost similar percentage of cellulose synthesis and degradation genes	1171:1240	All the species showed almost similar percentage of cellulose synthesis and degradation genes.
32681312	5	50	theme	microorganisms	540:553	arg1	discovery					521:529	the discovery	517:529	the discovery of novel microorganisms and their functions	517:573	The polar regions have attracted intense research interest for the discovery of novel microorganisms and their functions.
32681312	12	51	theme	hemicellulose	1333:1345	arg1	genes					1359:1363	hemicellulose degradation genes	1333:1363	hemicellulose degradation genes	1333:1363	However, the polar lichen-associated microorganism was found to have a high percentage of hemicellulose degradation genes, and less starch and laminarin degradation.
32681312	0	52	theme	sp	66:67	arg1	Study					20:24	Comparative Genomic Study	0:24	Comparative Genomic Study of Polar Lichen-Associated Hymenobacter sp	0:67	Comparative Genomic Study of Polar Lichen-Associated Hymenobacter sp.
32681312	13	53	theme	higher	1439:1444	arg1	number					1446:1451	higher number	1439:1451	higher number of hemicellulose degradation genes	1439:1486	The Hymenobacter species with higher number of hemicellulose degradation genes was found to have a lower number of starch and laminarin degradation genes and vice versa, highlighting the differences in polysaccharide utilization among the species.
32681312	13	54	theme	starch	1524:1529	arg1	genes					1557:1561	starch and laminarin degradation genes	1524:1561	starch and laminarin degradation genes	1524:1561	The Hymenobacter species with higher number of hemicellulose degradation genes was found to have a lower number of starch and laminarin degradation genes and vice versa, highlighting the differences in polysaccharide utilization among the species.
32681312	8	55	theme	PAMC	751:754	arg1	26628					756:760	PAMC 26628	751:760	PAMC 26628	751:760	PAMC 26628 was performed, along with its comparison with the complete genome of the same genus available in the NCBI database.
32681312	13	56	theme	lower	1508:1512	arg1	number					1514:1519	a lower number	1506:1519	a lower number of starch and laminarin degradation genes	1506:1561	The Hymenobacter species with higher number of hemicellulose degradation genes was found to have a lower number of starch and laminarin degradation genes and vice versa, highlighting the differences in polysaccharide utilization among the species.
32681312	0	57	theme	Hymenobacter	53:64	arg1	sp					66:67	Polar Lichen-Associated Hymenobacter sp	29:67	Polar Lichen-Associated Hymenobacter sp	29:67	Comparative Genomic Study of Polar Lichen-Associated Hymenobacter sp.
32681312	9	58	theme	AZCL	917:920	arg1	data					932:935	the AZCL screening data	913:935	the AZCL screening data for the two polar lichen-associated species	913:979	The study was conducted relying on the AZCL screening data for the two polar lichen-associated species.
32681312	10	59	theme	polysaccharide	1048:1061	arg1	preferences					1063:1073	polysaccharide preferences	1048:1073	polysaccharide preferences based on the isolation environment and biosample source	1048:1129	While comparing with eight other complete genomes, differences in polysaccharide preferences based on the isolation environment and biosample source were discovered.
32681312	12	60	theme	lichen-associated	1262:1278	arg1	microorganism					1280:1292	the polar lichen-associated microorganism	1252:1292	the polar lichen-associated microorganism	1252:1292	However, the polar lichen-associated microorganism was found to have a high percentage of hemicellulose degradation genes, and less starch and laminarin degradation.
32681312	9	61	theme	screening	922:930	arg1	data					932:935	the AZCL screening data	913:935	the AZCL screening data for the two polar lichen-associated species	913:979	The study was conducted relying on the AZCL screening data for the two polar lichen-associated species.
32681312	10	62	theme	isolation	1088:1096	arg1	environment					1098:1108	the isolation environment	1084:1108	the isolation environment	1084:1108	While comparing with eight other complete genomes, differences in polysaccharide preferences based on the isolation environment and biosample source were discovered.
32681312	8	63	with	comparison	792:801	arg1	genome					821:826	the complete genome	808:826	the complete genome of the same genus available in the NCBI database	808:875	PAMC 26628 was performed, along with its comparison with the complete genome of the same genus available in the NCBI database.
32681312	5	64	theme	functions	565:573	arg1	discovery					521:529	the discovery	517:529	the discovery of novel microorganisms and their functions	517:573	The polar regions have attracted intense research interest for the discovery of novel microorganisms and their functions.
32681312	12	65	theme	high	1314:1317	arg1	percentage					1319:1328	a high percentage	1312:1328	a high percentage of hemicellulose degradation genes	1312:1363	However, the polar lichen-associated microorganism was found to have a high percentage of hemicellulose degradation genes, and less starch and laminarin degradation.
32681312	6	66	theme	Hymenobacter	702:713	arg1	sp					715:716	Hymenobacter sp	702:716	Hymenobacter sp	702:716	Analysis of the polysaccharide utilization-related carbohydrate-active enzyme among the two lichen-associated polar organisms Hymenobacter sp.
32681312	8	67	from	available	846:854	arg1	database					868:875	the NCBI database	859:875	the NCBI database	859:875	PAMC 26628 was performed, along with its comparison with the complete genome of the same genus available in the NCBI database.
32681312	11	68	theme	synthesis	1210:1218	arg1	percentage					1186:1195	almost similar percentage	1171:1195	almost similar percentage of cellulose synthesis and degradation genes	1171:1240	All the species showed almost similar percentage of cellulose synthesis and degradation genes.
32681312	12	69	contain	have	1307:1310	arg2	starch					1375:1380	starch	1375:1380	starch	1375:1380	However, the polar lichen-associated microorganism was found to have a high percentage of hemicellulose degradation genes, and less starch and laminarin degradation.
32681312	12	69	contain	have	1307:1310	arg1	microorganism					1280:1292	the polar lichen-associated microorganism	1252:1292	the polar lichen-associated microorganism	1252:1292	However, the polar lichen-associated microorganism was found to have a high percentage of hemicellulose degradation genes, and less starch and laminarin degradation.
32681312	12	69	contain	have	1307:1310	arg2	percentage					1319:1328	a high percentage	1312:1328	a high percentage of hemicellulose degradation genes	1312:1363	However, the polar lichen-associated microorganism was found to have a high percentage of hemicellulose degradation genes, and less starch and laminarin degradation.
32681312	8	70	theme	NCBI	863:866	arg1	database					868:875	the NCBI database	859:875	the NCBI database	859:875	PAMC 26628 was performed, along with its comparison with the complete genome of the same genus available in the NCBI database.
32681312	4	71	theme	Arctic	424:429	arg1	regions					445:451	the Arctic and Antarctic regions	420:451	the Arctic and Antarctic regions	420:451	They have been isolated from diverse environments, such as air, soil, and lichen, along with extreme polar environments, including the Arctic and Antarctic regions.
32681312	13	72	theme	degradation	1545:1555	arg1	genes					1557:1561	starch and laminarin degradation genes	1524:1561	starch and laminarin degradation genes	1524:1561	The Hymenobacter species with higher number of hemicellulose degradation genes was found to have a lower number of starch and laminarin degradation genes and vice versa, highlighting the differences in polysaccharide utilization among the species.
32681312	13	73	with	species	1426:1432	arg1	number					1446:1451	higher number	1439:1451	higher number of hemicellulose degradation genes	1439:1486	The Hymenobacter species with higher number of hemicellulose degradation genes was found to have a lower number of starch and laminarin degradation genes and vice versa, highlighting the differences in polysaccharide utilization among the species.
32681312	6	74	theme	polar	686:690	arg1	organisms					692:700	the two lichen-associated polar organisms	660:700	the two lichen-associated polar organisms	660:700	Analysis of the polysaccharide utilization-related carbohydrate-active enzyme among the two lichen-associated polar organisms Hymenobacter sp.
32681312	13	75	theme	Hymenobacter	1413:1424	arg1	species					1426:1432	The Hymenobacter species	1409:1432	The Hymenobacter species with higher number of hemicellulose degradation genes	1409:1486	The Hymenobacter species with higher number of hemicellulose degradation genes was found to have a lower number of starch and laminarin degradation genes and vice versa, highlighting the differences in polysaccharide utilization among the species.
32681312	13	76	from	differences	1596:1606	arg1	utilization					1626:1636	polysaccharide utilization	1611:1636	polysaccharide utilization	1611:1636	The Hymenobacter species with higher number of hemicellulose degradation genes was found to have a lower number of starch and laminarin degradation genes and vice versa, highlighting the differences in polysaccharide utilization among the species.
32681312	10	77	theme	complete	1015:1022	arg1	genomes					1024:1030	eight other complete genomes	1003:1030	eight other complete genomes	1003:1030	While comparing with eight other complete genomes, differences in polysaccharide preferences based on the isolation environment and biosample source were discovered.
32681312	6	78	dep	sp	715:716	arg1	Analysis					576:583	Analysis	576:583	Analysis of the polysaccharide utilization-related carbohydrate-active enzyme among the two lichen-associated polar organisms	576:700	Analysis of the polysaccharide utilization-related carbohydrate-active enzyme among the two lichen-associated polar organisms Hymenobacter sp.
32681312	6	79	theme	enzyme	647:652	arg1	Analysis					576:583	Analysis	576:583	Analysis of the polysaccharide utilization-related carbohydrate-active enzyme among the two lichen-associated polar organisms	576:700	Analysis of the polysaccharide utilization-related carbohydrate-active enzyme among the two lichen-associated polar organisms Hymenobacter sp.
32681312	6	80	theme	lichen-associated	668:684	arg1	organisms					692:700	the two lichen-associated polar organisms	660:700	the two lichen-associated polar organisms	660:700	Analysis of the polysaccharide utilization-related carbohydrate-active enzyme among the two lichen-associated polar organisms Hymenobacter sp.
32681312	8	81	theme	complete	812:819	arg1	genome					821:826	the complete genome	808:826	the complete genome of the same genus available in the NCBI database	808:875	PAMC 26628 was performed, along with its comparison with the complete genome of the same genus available in the NCBI database.
33128033	7	0	theme	development	1381:1391	arg1	opportunities					1393:1405	in vitro diagnostic development opportunities	1361:1405	in vitro diagnostic development opportunities that utilize glycoforms as a disease-specific biomarkers	1361:1462	Moreover, the engineered mice can be used as a host to produce various aglycosylated antibodies in a convenient and robust fashion, thereby expanding in vitro diagnostic development opportunities that utilize glycoforms as a disease-specific biomarkers.
33128033	6	1	theme	accessible	1114:1123	arg1	platform					1137:1144	a robust, versatile and accessible immunoassay platform	1090:1144	a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics	1090:1208	Thus, this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method can provide a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics.
33128033	7	2	theme	aglycosylated	1282:1294	arg1	antibodies					1296:1305	various aglycosylated antibodies	1274:1305	various aglycosylated antibodies	1274:1305	Moreover, the engineered mice can be used as a host to produce various aglycosylated antibodies in a convenient and robust fashion, thereby expanding in vitro diagnostic development opportunities that utilize glycoforms as a disease-specific biomarkers.
33128033	4	3	theme	aglycosylated	795:807	arg1	mice					828:831	aglycosylated antibody-producing mice	795:831	aglycosylated antibody-producing mice	795:831	Here, we edited four murine immunoglobulin G genes using adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods to generate aglycosylated antibody-producing mice.
33128033	5	4	theme	required	876:883	arg1	performances					896:907	required analytical performances	876:907	required analytical performances	876:907	Resulting aglycosylated antibodies showed required analytical performances without compromised protein stability.
33128033	4	5	theme	immunoglobulin	661:674	arg1	genes					678:682	four murine immunoglobulin G genes	649:682	four murine immunoglobulin G genes	649:682	Here, we edited four murine immunoglobulin G genes using adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods to generate aglycosylated antibody-producing mice.
33128033	5	6	theme	compromised	917:927	arg1	stability					937:945	compromised protein stability	917:945	compromised protein stability	917:945	Resulting aglycosylated antibodies showed required analytical performances without compromised protein stability.
33128033	6	7	theme	monoclonal	973:982	arg1	immunoassay					1008:1018	monoclonal antibody-lectin coupled immunoassay	973:1018	this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method	954:1076	Thus, this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method can provide a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics.
33128033	6	7	theme	monoclonal	973:982	arg1	ALIQUAT					1062:1068	ALIQUAT	1062:1068	ALIQUAT	1062:1068	Thus, this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method can provide a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics.
33128033	3	8	theme	onerous	593:599	arg1	methods					624:630	incomplete, uneconomical and onerous enzymatic and chemical methods	564:630	incomplete, uneconomical and onerous enzymatic and chemical methods	564:630	Previously, aglycosylated antibody acquisition relied on incomplete, uneconomical and onerous enzymatic and chemical methods.
33128033	1	9	theme	routine	272:278	arg1	use					289:291	routine clinical use	272:291	routine clinical use	272:291	Targeting aberrant glycoforms has been validated for in vitro cancer diagnostic development, and several assays are currently in routine clinical use.
33128033	0	10	theme	markers	124:130	arg1	quantification					100:113	the quantification	96:113	the quantification of tumor markers (ALIQUAT)	96:140	Aglycosylated antibody-producing mice for aglycosylated antibody-lectin coupled immunoassay for the quantification of tumor markers (ALIQUAT).
33128033	3	11	theme	enzymatic	601:609	arg1	methods					624:630	incomplete, uneconomical and onerous enzymatic and chemical methods	564:630	incomplete, uneconomical and onerous enzymatic and chemical methods	564:630	Previously, aglycosylated antibody acquisition relied on incomplete, uneconomical and onerous enzymatic and chemical methods.
33128033	1	12	theme	clinical	280:287	arg1	use					289:291	routine clinical use	272:291	routine clinical use	272:291	Targeting aberrant glycoforms has been validated for in vitro cancer diagnostic development, and several assays are currently in routine clinical use.
33128033	0	13	theme	tumor	118:122	arg1	markers					124:130	tumor markers	118:130	tumor markers (ALIQUAT)	118:140	Aglycosylated antibody-producing mice for aglycosylated antibody-lectin coupled immunoassay for the quantification of tumor markers (ALIQUAT).
33128033	0	13	theme	tumor	118:122	arg1	ALIQUAT					133:139	ALIQUAT	133:139	ALIQUAT	133:139	Aglycosylated antibody-producing mice for aglycosylated antibody-lectin coupled immunoassay for the quantification of tumor markers (ALIQUAT).
33128033	7	14	theme	various	1274:1280	arg1	antibodies					1296:1305	various aglycosylated antibodies	1274:1305	various aglycosylated antibodies	1274:1305	Moreover, the engineered mice can be used as a host to produce various aglycosylated antibodies in a convenient and robust fashion, thereby expanding in vitro diagnostic development opportunities that utilize glycoforms as a disease-specific biomarkers.
33128033	3	15	theme	incomplete	564:573	arg1	methods					624:630	incomplete, uneconomical and onerous enzymatic and chemical methods	564:630	incomplete, uneconomical and onerous enzymatic and chemical methods	564:630	Previously, aglycosylated antibody acquisition relied on incomplete, uneconomical and onerous enzymatic and chemical methods.
33128033	4	16	theme	homology-directed	715:731	arg1	recombination					733:745	homology-directed recombination	715:745	homology-directed recombination	715:745	Here, we edited four murine immunoglobulin G genes using adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods to generate aglycosylated antibody-producing mice.
33128033	3	17	theme	chemical	615:622	arg1	methods					624:630	incomplete, uneconomical and onerous enzymatic and chemical methods	564:630	incomplete, uneconomical and onerous enzymatic and chemical methods	564:630	Previously, aglycosylated antibody acquisition relied on incomplete, uneconomical and onerous enzymatic and chemical methods.
33128033	4	18	theme	-mediated	752:760	arg1	methods					775:781	adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods	690:781	adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods	690:781	Here, we edited four murine immunoglobulin G genes using adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods to generate aglycosylated antibody-producing mice.
33128033	4	19	theme	G	676:676	arg1	genes					678:682	four murine immunoglobulin G genes	649:682	four murine immunoglobulin G genes	649:682	Here, we edited four murine immunoglobulin G genes using adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods to generate aglycosylated antibody-producing mice.
33128033	7	20	theme	convenient	1312:1321	arg1	fashion					1334:1340	a convenient and robust fashion	1310:1340	a convenient and robust fashion	1310:1340	Moreover, the engineered mice can be used as a host to produce various aglycosylated antibodies in a convenient and robust fashion, thereby expanding in vitro diagnostic development opportunities that utilize glycoforms as a disease-specific biomarkers.
33128033	4	21	theme	adenine	690:696	arg1	base-editing					698:709	adenine base-editing	690:709	adenine base-editing	690:709	Here, we edited four murine immunoglobulin G genes using adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods to generate aglycosylated antibody-producing mice.
33128033	1	22	theme	in	196:197	arg1	development					223:233	in vitro cancer diagnostic development	196:233	in vitro cancer diagnostic development	196:233	Targeting aberrant glycoforms has been validated for in vitro cancer diagnostic development, and several assays are currently in routine clinical use.
33128033	0	23	theme	antibody-producing	14:31	arg1	mice					33:36	Aglycosylated antibody-producing mice	0:36	Aglycosylated antibody-producing mice for aglycosylated antibody-lectin	0:70	Aglycosylated antibody-producing mice for aglycosylated antibody-lectin coupled immunoassay for the quantification of tumor markers (ALIQUAT).
33128033	6	24	theme	markers	1053:1059	arg1	quantification					1028:1041	the quantification	1024:1041	the quantification of tumour markers	1024:1059	Thus, this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method can provide a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics.
33128033	7	25	theme	disease-specific	1436:1451	arg1	biomarkers					1453:1462	a disease-specific biomarkers	1434:1462	a disease-specific biomarkers	1434:1462	Moreover, the engineered mice can be used as a host to produce various aglycosylated antibodies in a convenient and robust fashion, thereby expanding in vitro diagnostic development opportunities that utilize glycoforms as a disease-specific biomarkers.
33128033	2	26	theme	antibodies	328:337	arg1	region					318:323	Fc region	315:323	Fc region of antibodies	315:337	Because N-glycans in Fc region of antibodies show cross-reactivity with various lectins, high-quality aglycosylated antibodies are exceptionally important for immunoassay platform-based quantitative measurements.
33128033	7	27	theme	engineered	1225:1234	arg1	host					1258:1261	a host	1256:1261	a host	1256:1261	Moreover, the engineered mice can be used as a host to produce various aglycosylated antibodies in a convenient and robust fashion, thereby expanding in vitro diagnostic development opportunities that utilize glycoforms as a disease-specific biomarkers.
33128033	7	27	theme	engineered	1225:1234	arg1	mice					1236:1239	the engineered mice	1221:1239	the engineered mice	1221:1239	Moreover, the engineered mice can be used as a host to produce various aglycosylated antibodies in a convenient and robust fashion, thereby expanding in vitro diagnostic development opportunities that utilize glycoforms as a disease-specific biomarkers.
33128033	4	28	theme	editing	767:773	arg1	methods					775:781	adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods	690:781	adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods	690:781	Here, we edited four murine immunoglobulin G genes using adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods to generate aglycosylated antibody-producing mice.
33128033	0	29	theme	Aglycosylated	0:12	arg1	mice					33:36	Aglycosylated antibody-producing mice	0:36	Aglycosylated antibody-producing mice for aglycosylated antibody-lectin	0:70	Aglycosylated antibody-producing mice for aglycosylated antibody-lectin coupled immunoassay for the quantification of tumor markers (ALIQUAT).
33128033	5	30	gly	aglycosylated	844:856	arg1	antibodies					858:867	Resulting aglycosylated antibodies	834:867	Resulting aglycosylated antibodies	834:867	Resulting aglycosylated antibodies showed required analytical performances without compromised protein stability.
33128033	4	31	theme	gene	762:765	arg1	methods					775:781	adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods	690:781	adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods	690:781	Here, we edited four murine immunoglobulin G genes using adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods to generate aglycosylated antibody-producing mice.
33128033	7	32	used	used	1248:1251	arg2	mice					1236:1239	the engineered mice	1221:1239	the engineered mice	1221:1239	Moreover, the engineered mice can be used as a host to produce various aglycosylated antibodies in a convenient and robust fashion, thereby expanding in vitro diagnostic development opportunities that utilize glycoforms as a disease-specific biomarkers.
33128033	7	32	used	used	1248:1251	arg2	host					1258:1261	a host	1256:1261	a host	1256:1261	Moreover, the engineered mice can be used as a host to produce various aglycosylated antibodies in a convenient and robust fashion, thereby expanding in vitro diagnostic development opportunities that utilize glycoforms as a disease-specific biomarkers.
33128033	3	33	gly	aglycosylated	519:531	arg1	acquisition					542:552	aglycosylated antibody acquisition	519:552	aglycosylated antibody acquisition	519:552	Previously, aglycosylated antibody acquisition relied on incomplete, uneconomical and onerous enzymatic and chemical methods.
33128033	7	34	dep	in	1361:1362	arg1	vitro					1364:1368	vitro	1364:1368	vitro	1364:1368	Moreover, the engineered mice can be used as a host to produce various aglycosylated antibodies in a convenient and robust fashion, thereby expanding in vitro diagnostic development opportunities that utilize glycoforms as a disease-specific biomarkers.
33128033	2	35	theme	quantitative	480:491	arg1	measurements					493:504	immunoassay platform-based quantitative measurements	453:504	immunoassay platform-based quantitative measurements	453:504	Because N-glycans in Fc region of antibodies show cross-reactivity with various lectins, high-quality aglycosylated antibodies are exceptionally important for immunoassay platform-based quantitative measurements.
33128033	6	36	theme	immunoassay	1125:1135	arg1	platform					1137:1144	a robust, versatile and accessible immunoassay platform	1090:1144	a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics	1090:1208	Thus, this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method can provide a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics.
33128033	2	37	gly	aglycosylated	396:408	arg1	antibodies					410:419	high-quality aglycosylated antibodies	383:419	high-quality aglycosylated antibodies	383:419	Because N-glycans in Fc region of antibodies show cross-reactivity with various lectins, high-quality aglycosylated antibodies are exceptionally important for immunoassay platform-based quantitative measurements.
33128033	2	38	with	cross-reactivity	344:359	arg1	lectins					374:380	various lectins	366:380	various lectins	366:380	Because N-glycans in Fc region of antibodies show cross-reactivity with various lectins, high-quality aglycosylated antibodies are exceptionally important for immunoassay platform-based quantitative measurements.
33128033	2	39	theme	Fc	315:316	arg1	region					318:323	Fc region	315:323	Fc region of antibodies	315:337	Because N-glycans in Fc region of antibodies show cross-reactivity with various lectins, high-quality aglycosylated antibodies are exceptionally important for immunoassay platform-based quantitative measurements.
33128033	2	40	theme	platform-based	465:478	arg1	measurements					493:504	immunoassay platform-based quantitative measurements	453:504	immunoassay platform-based quantitative measurements	453:504	Because N-glycans in Fc region of antibodies show cross-reactivity with various lectins, high-quality aglycosylated antibodies are exceptionally important for immunoassay platform-based quantitative measurements.
33128033	5	41	theme	protein	929:935	arg1	stability					937:945	compromised protein stability	917:945	compromised protein stability	917:945	Resulting aglycosylated antibodies showed required analytical performances without compromised protein stability.
33128033	4	42	theme	antibody-producing	809:826	arg1	mice					828:831	aglycosylated antibody-producing mice	795:831	aglycosylated antibody-producing mice	795:831	Here, we edited four murine immunoglobulin G genes using adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods to generate aglycosylated antibody-producing mice.
33128033	3	43	theme	aglycosylated	519:531	arg1	acquisition					542:552	aglycosylated antibody acquisition	519:552	aglycosylated antibody acquisition	519:552	Previously, aglycosylated antibody acquisition relied on incomplete, uneconomical and onerous enzymatic and chemical methods.
33128033	0	44	gly	Aglycosylated	0:12	arg1	mice					33:36	Aglycosylated antibody-producing mice	0:36	Aglycosylated antibody-producing mice for aglycosylated antibody-lectin	0:70	Aglycosylated antibody-producing mice for aglycosylated antibody-lectin coupled immunoassay for the quantification of tumor markers (ALIQUAT).
33128033	2	45	theme	immunoassay	453:463	arg1	measurements					493:504	immunoassay platform-based quantitative measurements	453:504	immunoassay platform-based quantitative measurements	453:504	Because N-glycans in Fc region of antibodies show cross-reactivity with various lectins, high-quality aglycosylated antibodies are exceptionally important for immunoassay platform-based quantitative measurements.
33128033	0	46	theme	aglycosylated	42:54	arg1	antibody-lectin					56:70	aglycosylated antibody-lectin	42:70	aglycosylated antibody-lectin	42:70	Aglycosylated antibody-producing mice for aglycosylated antibody-lectin coupled immunoassay for the quantification of tumor markers (ALIQUAT).
33128033	6	47	gly	aglycosylated	959:971	arg1	method					1071:1076	this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method	954:1076	this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method	954:1076	Thus, this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method can provide a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics.
33128033	4	48	theme	murine	654:659	arg1	genes					678:682	four murine immunoglobulin G genes	649:682	four murine immunoglobulin G genes	649:682	Here, we edited four murine immunoglobulin G genes using adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods to generate aglycosylated antibody-producing mice.
33128033	7	49	theme	robust	1327:1332	arg1	fashion					1334:1340	a convenient and robust fashion	1310:1340	a convenient and robust fashion	1310:1340	Moreover, the engineered mice can be used as a host to produce various aglycosylated antibodies in a convenient and robust fashion, thereby expanding in vitro diagnostic development opportunities that utilize glycoforms as a disease-specific biomarkers.
33128033	3	50	theme	antibody	533:540	arg1	acquisition					542:552	aglycosylated antibody acquisition	519:552	aglycosylated antibody acquisition	519:552	Previously, aglycosylated antibody acquisition relied on incomplete, uneconomical and onerous enzymatic and chemical methods.
33128033	5	51	theme	aglycosylated	844:856	arg1	antibodies					858:867	Resulting aglycosylated antibodies	834:867	Resulting aglycosylated antibodies	834:867	Resulting aglycosylated antibodies showed required analytical performances without compromised protein stability.
33128033	7	52	gly	aglycosylated	1282:1294	arg1	antibodies					1296:1305	various aglycosylated antibodies	1274:1305	various aglycosylated antibodies	1274:1305	Moreover, the engineered mice can be used as a host to produce various aglycosylated antibodies in a convenient and robust fashion, thereby expanding in vitro diagnostic development opportunities that utilize glycoforms as a disease-specific biomarkers.
33128033	7	53	theme	diagnostic	1370:1379	arg1	opportunities					1393:1405	in vitro diagnostic development opportunities	1361:1405	in vitro diagnostic development opportunities that utilize glycoforms as a disease-specific biomarkers	1361:1462	Moreover, the engineered mice can be used as a host to produce various aglycosylated antibodies in a convenient and robust fashion, thereby expanding in vitro diagnostic development opportunities that utilize glycoforms as a disease-specific biomarkers.
33128033	6	54	theme	tumour	1046:1051	arg1	markers					1053:1059	tumour markers	1046:1059	tumour markers	1046:1059	Thus, this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method can provide a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics.
33128033	2	55	theme	various	366:372	arg1	lectins					374:380	various lectins	366:380	various lectins	366:380	Because N-glycans in Fc region of antibodies show cross-reactivity with various lectins, high-quality aglycosylated antibodies are exceptionally important for immunoassay platform-based quantitative measurements.
33128033	5	56	theme	Resulting	834:842	arg1	antibodies					858:867	Resulting aglycosylated antibodies	834:867	Resulting aglycosylated antibodies	834:867	Resulting aglycosylated antibodies showed required analytical performances without compromised protein stability.
33128033	1	57	gly	glycoforms	162:171	arg1	Targeting					143:151	Targeting aberrant glycoforms	143:171	Targeting aberrant glycoforms	143:171	Targeting aberrant glycoforms has been validated for in vitro cancer diagnostic development, and several assays are currently in routine clinical use.
33128033	1	58	dep	in	196:197	arg1	vitro					199:203	vitro	199:203	vitro	199:203	Targeting aberrant glycoforms has been validated for in vitro cancer diagnostic development, and several assays are currently in routine clinical use.
33128033	1	58	dep	in	196:197	arg1	diagnostic					212:221	diagnostic	212:221	diagnostic	212:221	Targeting aberrant glycoforms has been validated for in vitro cancer diagnostic development, and several assays are currently in routine clinical use.
33128033	7	59	theme	in	1361:1362	arg1	opportunities					1393:1405	in vitro diagnostic development opportunities	1361:1405	in vitro diagnostic development opportunities that utilize glycoforms as a disease-specific biomarkers	1361:1462	Moreover, the engineered mice can be used as a host to produce various aglycosylated antibodies in a convenient and robust fashion, thereby expanding in vitro diagnostic development opportunities that utilize glycoforms as a disease-specific biomarkers.
33128033	3	60	theme	uneconomical	576:587	arg1	methods					624:630	incomplete, uneconomical and onerous enzymatic and chemical methods	564:630	incomplete, uneconomical and onerous enzymatic and chemical methods	564:630	Previously, aglycosylated antibody acquisition relied on incomplete, uneconomical and onerous enzymatic and chemical methods.
33128033	6	61	theme	robust	1092:1097	arg1	platform					1137:1144	a robust, versatile and accessible immunoassay platform	1090:1144	a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics	1090:1208	Thus, this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method can provide a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics.
33128033	6	62	theme	immunoassay	1008:1018	arg1	method					1071:1076	this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method	954:1076	this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method	954:1076	Thus, this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method can provide a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics.
33128033	0	63	gly	aglycosylated	42:54	arg1	antibody-lectin					56:70	aglycosylated antibody-lectin	42:70	aglycosylated antibody-lectin	42:70	Aglycosylated antibody-producing mice for aglycosylated antibody-lectin coupled immunoassay for the quantification of tumor markers (ALIQUAT).
33128033	6	64	theme	aglycosylated	959:971	arg1	method					1071:1076	this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method	954:1076	this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method	954:1076	Thus, this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method can provide a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics.
33128033	1	65	theme	Targeting	143:151	arg1	glycoforms					162:171	Targeting aberrant glycoforms	143:171	Targeting aberrant glycoforms	143:171	Targeting aberrant glycoforms has been validated for in vitro cancer diagnostic development, and several assays are currently in routine clinical use.
33128033	1	66	theme	several	240:246	arg1	assays					248:253	several assays	240:253	several assays	240:253	Targeting aberrant glycoforms has been validated for in vitro cancer diagnostic development, and several assays are currently in routine clinical use.
33128033	4	67	gly	aglycosylated	795:807	arg1	mice					828:831	aglycosylated antibody-producing mice	795:831	aglycosylated antibody-producing mice	795:831	Here, we edited four murine immunoglobulin G genes using adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods to generate aglycosylated antibody-producing mice.
33128033	6	68	theme	coupled	1000:1006	arg1	immunoassay					1008:1018	monoclonal antibody-lectin coupled immunoassay	973:1018	this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method	954:1076	Thus, this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method can provide a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics.
33128033	6	68	theme	coupled	1000:1006	arg1	ALIQUAT					1062:1068	ALIQUAT	1062:1068	ALIQUAT	1062:1068	Thus, this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method can provide a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics.
33128033	1	69	theme	aberrant	153:160	arg1	glycoforms					162:171	Targeting aberrant glycoforms	143:171	Targeting aberrant glycoforms	143:171	Targeting aberrant glycoforms has been validated for in vitro cancer diagnostic development, and several assays are currently in routine clinical use.
33128033	5	70	theme	analytical	885:894	arg1	performances					896:907	required analytical performances	876:907	required analytical performances	876:907	Resulting aglycosylated antibodies showed required analytical performances without compromised protein stability.
33128033	6	71	theme	antibody-lectin	984:998	arg1	immunoassay					1008:1018	monoclonal antibody-lectin coupled immunoassay	973:1018	this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method	954:1076	Thus, this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method can provide a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics.
33128033	6	71	theme	antibody-lectin	984:998	arg1	ALIQUAT					1062:1068	ALIQUAT	1062:1068	ALIQUAT	1062:1068	Thus, this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method can provide a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics.
33128033	4	72	dep	-mediated	752:760	arg1	base-editing					698:709	adenine base-editing	690:709	adenine base-editing	690:709	Here, we edited four murine immunoglobulin G genes using adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods to generate aglycosylated antibody-producing mice.
33128033	4	72	dep	-mediated	752:760	arg1	recombination					733:745	homology-directed recombination	715:745	homology-directed recombination	715:745	Here, we edited four murine immunoglobulin G genes using adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods to generate aglycosylated antibody-producing mice.
33128033	2	73	theme	aglycosylated	396:408	arg1	antibodies					410:419	high-quality aglycosylated antibodies	383:419	high-quality aglycosylated antibodies	383:419	Because N-glycans in Fc region of antibodies show cross-reactivity with various lectins, high-quality aglycosylated antibodies are exceptionally important for immunoassay platform-based quantitative measurements.
33128033	2	74	from	N-glycans	302:310	arg1	region					318:323	Fc region	315:323	Fc region of antibodies	315:337	Because N-glycans in Fc region of antibodies show cross-reactivity with various lectins, high-quality aglycosylated antibodies are exceptionally important for immunoassay platform-based quantitative measurements.
33128033	6	75	theme	cancer	1191:1196	arg1	diagnostics					1198:1208	precision cancer diagnostics	1181:1208	precision cancer diagnostics	1181:1208	Thus, this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method can provide a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics.
33128033	6	76	theme	specific	1158:1165	arg1	glycoforms					1167:1176	specific glycoforms	1158:1176	specific glycoforms	1158:1176	Thus, this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method can provide a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics.
33128033	6	77	theme	precision	1181:1189	arg1	diagnostics					1198:1208	precision cancer diagnostics	1181:1208	precision cancer diagnostics	1181:1208	Thus, this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method can provide a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics.
33128033	2	78	theme	high-quality	383:394	arg1	antibodies					410:419	high-quality aglycosylated antibodies	383:419	high-quality aglycosylated antibodies	383:419	Because N-glycans in Fc region of antibodies show cross-reactivity with various lectins, high-quality aglycosylated antibodies are exceptionally important for immunoassay platform-based quantitative measurements.
33128033	6	79	theme	versatile	1100:1108	arg1	platform					1137:1144	a robust, versatile and accessible immunoassay platform	1090:1144	a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics	1090:1208	Thus, this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method can provide a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics.
34597753	0	0	theme	acid	87:90	arg1	coils					92:96	hyaluronic acid coils	76:96	hyaluronic acid coils	76:96	How centrifugation influences the recovery and soy peptone incorporation in hyaluronic acid coils from fermentation.
34597753	2	1	theme	bio-HA	506:511	arg1	interactions					513:524	the bio-HA interactions	502:524	the bio-HA interactions with the medium components	502:551	Bio-HA is a capsular exopolysaccharide highly hydrophilic with a coiled structure and high negative charge density at physiological pH. Centrifugation, currently used to separate microbial cells after fermentation, should significantly affect the bio-HA interactions with the medium components.
34597753	2	2	theme	charge	359:364	arg1	density					366:372	high negative charge density	345:372	high negative charge density	345:372	Bio-HA is a capsular exopolysaccharide highly hydrophilic with a coiled structure and high negative charge density at physiological pH. Centrifugation, currently used to separate microbial cells after fermentation, should significantly affect the bio-HA interactions with the medium components.
34597753	1	3	theme	medical	244:250	arg1	areas					252:256	aesthetic and medical areas	230:256	aesthetic and medical areas	230:256	Hyaluronic acid from fermentation (bio-HA) is a high-added-value biopolymer with a wide range of applications in aesthetic and medical areas.
34597753	0	4	theme	hyaluronic	76:85	arg1	coils					92:96	hyaluronic acid coils	76:96	hyaluronic acid coils	76:96	How centrifugation influences the recovery and soy peptone incorporation in hyaluronic acid coils from fermentation.
34597753	4	5	with	interactions	764:775	arg1	SP					795:796	SP	795:796	SP	795:796	We examined how centrifugation influences its recovery and characterized interactions with soy peptone (SP), used as a nitrogen source.
34597753	4	5	with	interactions	764:775	arg1	peptone					786:792	soy peptone	782:792	soy peptone (SP)	782:797	We examined how centrifugation influences its recovery and characterized interactions with soy peptone (SP), used as a nitrogen source.
34597753	2	6	theme	negative	350:357	arg1	density					366:372	high negative charge density	345:372	high negative charge density	345:372	Bio-HA is a capsular exopolysaccharide highly hydrophilic with a coiled structure and high negative charge density at physiological pH. Centrifugation, currently used to separate microbial cells after fermentation, should significantly affect the bio-HA interactions with the medium components.
34597753	7	7	from	higher	1259:1264	arg1	sedimentation					1242:1254	sedimentation	1242:1254	sedimentation at higher spins and times	1242:1280	The incorporation of amino acids and peptides from SP increased HA coils' density, causing losses due to sedimentation at higher spins and times.
34597753	7	8	dep	higher	1259:1264	arg1	spins					1266:1270	spins	1266:1270	spins	1266:1270	The incorporation of amino acids and peptides from SP increased HA coils' density, causing losses due to sedimentation at higher spins and times.
34597753	7	9	theme	due	1235:1237	arg1	losses					1228:1233	losses	1228:1233	losses due to sedimentation at higher spins and times	1228:1280	The incorporation of amino acids and peptides from SP increased HA coils' density, causing losses due to sedimentation at higher spins and times.
34597753	2	10	theme	capsular	271:278	arg1	exopolysaccharide					280:296	a capsular exopolysaccharide	269:296	a capsular exopolysaccharide highly hydrophilic	269:315	Bio-HA is a capsular exopolysaccharide highly hydrophilic with a coiled structure and high negative charge density at physiological pH. Centrifugation, currently used to separate microbial cells after fermentation, should significantly affect the bio-HA interactions with the medium components.
34597753	2	10	theme	capsular	271:278	arg1	Bio-HA					259:264	Bio-HA	259:264	Bio-HA	259:264	Bio-HA is a capsular exopolysaccharide highly hydrophilic with a coiled structure and high negative charge density at physiological pH. Centrifugation, currently used to separate microbial cells after fermentation, should significantly affect the bio-HA interactions with the medium components.
34597753	2	11	theme	medium	535:540	arg1	components					542:551	the medium components	531:551	the medium components	531:551	Bio-HA is a capsular exopolysaccharide highly hydrophilic with a coiled structure and high negative charge density at physiological pH. Centrifugation, currently used to separate microbial cells after fermentation, should significantly affect the bio-HA interactions with the medium components.
34597753	2	12	theme	high	345:348	arg1	density					366:372	high negative charge density	345:372	high negative charge density	345:372	Bio-HA is a capsular exopolysaccharide highly hydrophilic with a coiled structure and high negative charge density at physiological pH. Centrifugation, currently used to separate microbial cells after fermentation, should significantly affect the bio-HA interactions with the medium components.
34597753	2	13	with	interactions	513:524	arg1	components					542:551	the medium components	531:551	the medium components	531:551	Bio-HA is a capsular exopolysaccharide highly hydrophilic with a coiled structure and high negative charge density at physiological pH. Centrifugation, currently used to separate microbial cells after fermentation, should significantly affect the bio-HA interactions with the medium components.
34597753	5	14	theme	composite	893:901	arg1	design					903:908	a statistical central composite design	871:908	a statistical central composite design (CCD) planning with replicates at the central point with time (8-22 min) and spin (582xg to 12,402xg) as independent variables	871:1035	We collected experimental data according to a statistical central composite design (CCD) planning with replicates at the central point with time (8-22 min) and spin (582xg to 12,402xg) as independent variables.
34597753	5	14	theme	composite	893:901	arg1	CCD					911:913	CCD	911:913	CCD	911:913	We collected experimental data according to a statistical central composite design (CCD) planning with replicates at the central point with time (8-22 min) and spin (582xg to 12,402xg) as independent variables.
34597753	5	15	with	point	956:960	arg1	12,402xg					1002:1009	582xg to 12,402xg	993:1009	582xg to 12,402xg	993:1009	We collected experimental data according to a statistical central composite design (CCD) planning with replicates at the central point with time (8-22 min) and spin (582xg to 12,402xg) as independent variables.
34597753	5	15	with	point	956:960	arg1	8-22 min					973:980	8-22 min	973:980	8-22 min	973:980	We collected experimental data according to a statistical central composite design (CCD) planning with replicates at the central point with time (8-22 min) and spin (582xg to 12,402xg) as independent variables.
34597753	5	15	with	point	956:960	arg1	spin					987:990	spin	987:990	spin (582xg to 12,402xg)	987:1010	We collected experimental data according to a statistical central composite design (CCD) planning with replicates at the central point with time (8-22 min) and spin (582xg to 12,402xg) as independent variables.
34597753	5	15	with	point	956:960	arg1	time					967:970	time	967:970	time (8-22 min)	967:981	We collected experimental data according to a statistical central composite design (CCD) planning with replicates at the central point with time (8-22 min) and spin (582xg to 12,402xg) as independent variables.
34597753	1	16	theme	high-added-value	165:180	arg1	biopolymer					182:191	a high-added-value biopolymer	163:191	a high-added-value biopolymer with a wide range of applications in aesthetic and medical areas	163:256	Hyaluronic acid from fermentation (bio-HA) is a high-added-value biopolymer with a wide range of applications in aesthetic and medical areas.
34597753	1	16	theme	high-added-value	165:180	arg1	acid					128:131	Hyaluronic acid	117:131	Hyaluronic acid from fermentation (bio-HA)	117:158	Hyaluronic acid from fermentation (bio-HA) is a high-added-value biopolymer with a wide range of applications in aesthetic and medical areas.
34597753	7	17	theme	peptides	1174:1181	arg1	incorporation					1141:1153	The incorporation	1137:1153	The incorporation of amino acids and peptides from SP	1137:1189	The incorporation of amino acids and peptides from SP increased HA coils' density, causing losses due to sedimentation at higher spins and times.
34597753	0	18	from	recovery	34:41	arg1	coils					92:96	hyaluronic acid coils	76:96	hyaluronic acid coils	76:96	How centrifugation influences the recovery and soy peptone incorporation in hyaluronic acid coils from fermentation.
34597753	8	19	theme	molar	1368:1372	arg1	mass					1374:1377	molar mass	1368:1377	molar mass 106,105 and 104 Da	1368:1396	Furthermore, SP incorporation also changed the coils' size regarding HA standards of molar mass 106,105 and 104 Da.
34597753	1	20	from	fermentation	138:149	arg1	biopolymer					182:191	a high-added-value biopolymer	163:191	a high-added-value biopolymer with a wide range of applications in aesthetic and medical areas	163:256	Hyaluronic acid from fermentation (bio-HA) is a high-added-value biopolymer with a wide range of applications in aesthetic and medical areas.
34597753	1	20	from	fermentation	138:149	arg1	acid					128:131	Hyaluronic acid	117:131	Hyaluronic acid from fermentation (bio-HA)	117:158	Hyaluronic acid from fermentation (bio-HA) is a high-added-value biopolymer with a wide range of applications in aesthetic and medical areas.
34597753	8	21	theme	HA	1352:1353	arg1	standards					1355:1363	HA standards	1352:1363	HA standards of molar mass 106,105 and 104 Da	1352:1396	Furthermore, SP incorporation also changed the coils' size regarding HA standards of molar mass 106,105 and 104 Da.
34597753	2	22	theme	pH.	391:393	arg1	Centrifugation					395:408	physiological pH. Centrifugation	377:408	physiological pH. Centrifugation	377:408	Bio-HA is a capsular exopolysaccharide highly hydrophilic with a coiled structure and high negative charge density at physiological pH. Centrifugation, currently used to separate microbial cells after fermentation, should significantly affect the bio-HA interactions with the medium components.
34597753	5	23	theme	experimental	840:851	arg1	data					853:856	experimental data	840:856	experimental data	840:856	We collected experimental data according to a statistical central composite design (CCD) planning with replicates at the central point with time (8-22 min) and spin (582xg to 12,402xg) as independent variables.
34597753	7	24	from	incorporation	1141:1153	arg1	SP					1188:1189	SP	1188:1189	SP	1188:1189	The incorporation of amino acids and peptides from SP increased HA coils' density, causing losses due to sedimentation at higher spins and times.
34597753	2	25	theme	physiological	377:389	arg1	Centrifugation					395:408	physiological pH. Centrifugation	377:408	physiological pH. Centrifugation	377:408	Bio-HA is a capsular exopolysaccharide highly hydrophilic with a coiled structure and high negative charge density at physiological pH. Centrifugation, currently used to separate microbial cells after fermentation, should significantly affect the bio-HA interactions with the medium components.
34597753	7	26	theme	acids	1164:1168	arg1	incorporation					1141:1153	The incorporation	1137:1153	The incorporation of amino acids and peptides from SP	1137:1189	The incorporation of amino acids and peptides from SP increased HA coils' density, causing losses due to sedimentation at higher spins and times.
34597753	8	27	dep	mass	1374:1377	arg1	Da					1395:1396	106,105 and 104 Da	1379:1396	molar mass 106,105 and 104 Da	1368:1396	Furthermore, SP incorporation also changed the coils' size regarding HA standards of molar mass 106,105 and 104 Da.
34597753	2	28	theme	hydrophilic	305:315	arg1	exopolysaccharide					280:296	a capsular exopolysaccharide	269:296	a capsular exopolysaccharide highly hydrophilic	269:315	Bio-HA is a capsular exopolysaccharide highly hydrophilic with a coiled structure and high negative charge density at physiological pH. Centrifugation, currently used to separate microbial cells after fermentation, should significantly affect the bio-HA interactions with the medium components.
34597753	2	28	theme	hydrophilic	305:315	arg1	Bio-HA					259:264	Bio-HA	259:264	Bio-HA	259:264	Bio-HA is a capsular exopolysaccharide highly hydrophilic with a coiled structure and high negative charge density at physiological pH. Centrifugation, currently used to separate microbial cells after fermentation, should significantly affect the bio-HA interactions with the medium components.
34597753	8	29	theme	mass	1374:1377	arg1	standards					1355:1363	HA standards	1352:1363	HA standards of molar mass 106,105 and 104 Da	1352:1396	Furthermore, SP incorporation also changed the coils' size regarding HA standards of molar mass 106,105 and 104 Da.
34597753	2	30	theme	separate	429:436	arg1	cells					448:452	separate microbial cells	429:452	separate microbial cells	429:452	Bio-HA is a capsular exopolysaccharide highly hydrophilic with a coiled structure and high negative charge density at physiological pH. Centrifugation, currently used to separate microbial cells after fermentation, should significantly affect the bio-HA interactions with the medium components.
34597753	2	31	theme	microbial	438:446	arg1	cells					448:452	separate microbial cells	429:452	separate microbial cells	429:452	Bio-HA is a capsular exopolysaccharide highly hydrophilic with a coiled structure and high negative charge density at physiological pH. Centrifugation, currently used to separate microbial cells after fermentation, should significantly affect the bio-HA interactions with the medium components.
34597753	7	32	from	SP	1188:1189	arg1	peptides					1174:1181	peptides	1174:1181	peptides from SP	1174:1189	The incorporation of amino acids and peptides from SP increased HA coils' density, causing losses due to sedimentation at higher spins and times.
34597753	7	32	from	SP	1188:1189	arg1	incorporation					1141:1153	The incorporation	1137:1153	The incorporation of amino acids and peptides from SP	1137:1189	The incorporation of amino acids and peptides from SP increased HA coils' density, causing losses due to sedimentation at higher spins and times.
34597753	7	32	from	SP	1188:1189	arg1	acids					1164:1168	amino acids	1158:1168	amino acids	1158:1168	The incorporation of amino acids and peptides from SP increased HA coils' density, causing losses due to sedimentation at higher spins and times.
34597753	3	33	theme	centrifugation	592:605	arg1	conditions					607:616	various centrifugation conditions	584:616	various centrifugation conditions	584:616	Although the literature shows various centrifugation conditions for bio-HA, there is no concern about its recovery and characterization.
34597753	1	34	theme	wide	200:203	arg1	range					205:209	a wide range	198:209	a wide range of applications in aesthetic and medical areas	198:256	Hyaluronic acid from fermentation (bio-HA) is a high-added-value biopolymer with a wide range of applications in aesthetic and medical areas.
34597753	6	35	theme	obtained	1042:1049	arg1	results					1051:1057	The obtained results	1038:1057	The obtained results	1038:1057	The obtained results show bio-HA recoveries ranged from 45 ± 0.5-100% as dosed in the supernatant.
34597753	6	36	dep	%	1106:1106	arg1	dosed					1111:1115	dosed	1111:1115	dosed in the supernatant	1111:1134	The obtained results show bio-HA recoveries ranged from 45 ± 0.5-100% as dosed in the supernatant.
34597753	6	37	theme	bio-HA	1064:1069	arg1	recoveries					1071:1080	bio-HA recoveries	1064:1080	bio-HA recoveries ranged from 45 ± 0.5-100% as dosed in the supernatant	1064:1134	The obtained results show bio-HA recoveries ranged from 45 ± 0.5-100% as dosed in the supernatant.
34597753	5	38	theme	statistical	873:883	arg1	design					903:908	a statistical central composite design	871:908	a statistical central composite design (CCD) planning with replicates at the central point with time (8-22 min) and spin (582xg to 12,402xg) as independent variables	871:1035	We collected experimental data according to a statistical central composite design (CCD) planning with replicates at the central point with time (8-22 min) and spin (582xg to 12,402xg) as independent variables.
34597753	5	38	theme	statistical	873:883	arg1	CCD					911:913	CCD	911:913	CCD	911:913	We collected experimental data according to a statistical central composite design (CCD) planning with replicates at the central point with time (8-22 min) and spin (582xg to 12,402xg) as independent variables.
34597753	7	39	theme	amino	1158:1162	arg1	acids					1164:1168	amino acids	1158:1168	amino acids	1158:1168	The incorporation of amino acids and peptides from SP increased HA coils' density, causing losses due to sedimentation at higher spins and times.
34597753	1	40	from	areas	252:256	arg1	range					205:209	a wide range	198:209	a wide range of applications in aesthetic and medical areas	198:256	Hyaluronic acid from fermentation (bio-HA) is a high-added-value biopolymer with a wide range of applications in aesthetic and medical areas.
34597753	2	41	with	exopolysaccharide	280:296	arg1	density					366:372	high negative charge density	345:372	high negative charge density	345:372	Bio-HA is a capsular exopolysaccharide highly hydrophilic with a coiled structure and high negative charge density at physiological pH. Centrifugation, currently used to separate microbial cells after fermentation, should significantly affect the bio-HA interactions with the medium components.
34597753	2	41	with	exopolysaccharide	280:296	arg1	structure					331:339	a coiled structure	322:339	a coiled structure	322:339	Bio-HA is a capsular exopolysaccharide highly hydrophilic with a coiled structure and high negative charge density at physiological pH. Centrifugation, currently used to separate microbial cells after fermentation, should significantly affect the bio-HA interactions with the medium components.
34597753	1	42	from	range	205:209	arg1	areas					252:256	aesthetic and medical areas	230:256	aesthetic and medical areas	230:256	Hyaluronic acid from fermentation (bio-HA) is a high-added-value biopolymer with a wide range of applications in aesthetic and medical areas.
34597753	5	43	theme	central	885:891	arg1	design					903:908	a statistical central composite design	871:908	a statistical central composite design (CCD) planning with replicates at the central point with time (8-22 min) and spin (582xg to 12,402xg) as independent variables	871:1035	We collected experimental data according to a statistical central composite design (CCD) planning with replicates at the central point with time (8-22 min) and spin (582xg to 12,402xg) as independent variables.
34597753	5	43	theme	central	885:891	arg1	CCD					911:913	CCD	911:913	CCD	911:913	We collected experimental data according to a statistical central composite design (CCD) planning with replicates at the central point with time (8-22 min) and spin (582xg to 12,402xg) as independent variables.
34597753	0	44	theme	peptone	51:57	arg1	incorporation					59:71	soy peptone incorporation	47:71	soy peptone incorporation	47:71	How centrifugation influences the recovery and soy peptone incorporation in hyaluronic acid coils from fermentation.
34597753	1	45	with	biopolymer	182:191	arg1	range					205:209	a wide range	198:209	a wide range of applications in aesthetic and medical areas	198:256	Hyaluronic acid from fermentation (bio-HA) is a high-added-value biopolymer with a wide range of applications in aesthetic and medical areas.
34597753	0	46	from	incorporation	59:71	arg1	coils					92:96	hyaluronic acid coils	76:96	hyaluronic acid coils	76:96	How centrifugation influences the recovery and soy peptone incorporation in hyaluronic acid coils from fermentation.
34597753	7	47	from	times	1276:1280	arg1	sedimentation					1242:1254	sedimentation	1242:1254	sedimentation at higher spins and times	1242:1280	The incorporation of amino acids and peptides from SP increased HA coils' density, causing losses due to sedimentation at higher spins and times.
34597753	9	48	theme	production	1505:1514	arg1	cost					1490:1493	the total cost	1480:1493	the total cost of bio-HA production	1480:1514	We conclude centrifugation should significantly impact downstream processing and the total cost of bio-HA production.
34597753	9	48	theme	production	1505:1514	arg1	processing					1465:1474	downstream processing	1454:1474	downstream processing	1454:1474	We conclude centrifugation should significantly impact downstream processing and the total cost of bio-HA production.
34597753	1	49	theme	Hyaluronic	117:126	arg1	biopolymer					182:191	a high-added-value biopolymer	163:191	a high-added-value biopolymer with a wide range of applications in aesthetic and medical areas	163:256	Hyaluronic acid from fermentation (bio-HA) is a high-added-value biopolymer with a wide range of applications in aesthetic and medical areas.
34597753	1	49	theme	Hyaluronic	117:126	arg1	acid					128:131	Hyaluronic acid	117:131	Hyaluronic acid from fermentation (bio-HA)	117:158	Hyaluronic acid from fermentation (bio-HA) is a high-added-value biopolymer with a wide range of applications in aesthetic and medical areas.
34597753	0	50	theme	soy	47:49	arg1	incorporation					59:71	soy peptone incorporation	47:71	soy peptone incorporation	47:71	How centrifugation influences the recovery and soy peptone incorporation in hyaluronic acid coils from fermentation.
34597753	0	51	dep	recovery	34:41	arg1	the					30:32	the	30:32	the	30:32	How centrifugation influences the recovery and soy peptone incorporation in hyaluronic acid coils from fermentation.
34597753	9	52	theme	downstream	1454:1463	arg1	processing					1465:1474	downstream processing	1454:1474	downstream processing	1454:1474	We conclude centrifugation should significantly impact downstream processing and the total cost of bio-HA production.
34597753	9	53	theme	total	1484:1488	arg1	cost					1490:1493	the total cost	1480:1493	the total cost of bio-HA production	1480:1514	We conclude centrifugation should significantly impact downstream processing and the total cost of bio-HA production.
34597753	1	54	from	applications	214:225	arg1	areas					252:256	aesthetic and medical areas	230:256	aesthetic and medical areas	230:256	Hyaluronic acid from fermentation (bio-HA) is a high-added-value biopolymer with a wide range of applications in aesthetic and medical areas.
34597753	0	55	from	fermentation	103:114	arg1	incorporation					59:71	soy peptone incorporation	47:71	soy peptone incorporation	47:71	How centrifugation influences the recovery and soy peptone incorporation in hyaluronic acid coils from fermentation.
34597753	0	55	from	fermentation	103:114	arg1	recovery					34:41	recovery	34:41	recovery	34:41	How centrifugation influences the recovery and soy peptone incorporation in hyaluronic acid coils from fermentation.
34597753	5	56	theme	582xg	993:997	arg1	12,402xg					1002:1009	582xg to 12,402xg	993:1009	582xg to 12,402xg	993:1009	We collected experimental data according to a statistical central composite design (CCD) planning with replicates at the central point with time (8-22 min) and spin (582xg to 12,402xg) as independent variables.
34597753	5	56	theme	582xg	993:997	arg1	spin					987:990	spin	987:990	spin (582xg to 12,402xg)	987:1010	We collected experimental data according to a statistical central composite design (CCD) planning with replicates at the central point with time (8-22 min) and spin (582xg to 12,402xg) as independent variables.
34597753	5	57	theme	independent	1015:1025	arg1	variables					1027:1035	independent variables	1015:1035	independent variables	1015:1035	We collected experimental data according to a statistical central composite design (CCD) planning with replicates at the central point with time (8-22 min) and spin (582xg to 12,402xg) as independent variables.
34597753	4	58	theme	soy	782:784	arg1	SP					795:796	SP	795:796	SP	795:796	We examined how centrifugation influences its recovery and characterized interactions with soy peptone (SP), used as a nitrogen source.
34597753	4	58	theme	soy	782:784	arg1	peptone					786:792	soy peptone	782:792	soy peptone (SP)	782:797	We examined how centrifugation influences its recovery and characterized interactions with soy peptone (SP), used as a nitrogen source.
34597753	3	59	theme	various	584:590	arg1	conditions					607:616	various centrifugation conditions	584:616	various centrifugation conditions	584:616	Although the literature shows various centrifugation conditions for bio-HA, there is no concern about its recovery and characterization.
34597753	1	60	theme	applications	214:225	arg1	range					205:209	a wide range	198:209	a wide range of applications in aesthetic and medical areas	198:256	Hyaluronic acid from fermentation (bio-HA) is a high-added-value biopolymer with a wide range of applications in aesthetic and medical areas.
34597753	4	61	theme	nitrogen	810:817	arg1	source					819:824	a nitrogen source	808:824	a nitrogen source	808:824	We examined how centrifugation influences its recovery and characterized interactions with soy peptone (SP), used as a nitrogen source.
34597753	2	62	theme	coiled	324:329	arg1	structure					331:339	a coiled structure	322:339	a coiled structure	322:339	Bio-HA is a capsular exopolysaccharide highly hydrophilic with a coiled structure and high negative charge density at physiological pH. Centrifugation, currently used to separate microbial cells after fermentation, should significantly affect the bio-HA interactions with the medium components.
34597753	5	63	theme	central	948:954	arg1	point					956:960	the central point	944:960	the central point with time (8-22 min) and spin (582xg to 12,402xg)	944:1010	We collected experimental data according to a statistical central composite design (CCD) planning with replicates at the central point with time (8-22 min) and spin (582xg to 12,402xg) as independent variables.
34597753	5	64	dep	12,402xg	1002:1009	arg1	to					999:1000	to	999:1000	to	999:1000	We collected experimental data according to a statistical central composite design (CCD) planning with replicates at the central point with time (8-22 min) and spin (582xg to 12,402xg) as independent variables.
34597753	9	65	theme	bio-HA	1498:1503	arg1	production					1505:1514	bio-HA production	1498:1514	bio-HA production	1498:1514	We conclude centrifugation should significantly impact downstream processing and the total cost of bio-HA production.
34597753	1	66	theme	aesthetic	230:238	arg1	areas					252:256	aesthetic and medical areas	230:256	aesthetic and medical areas	230:256	Hyaluronic acid from fermentation (bio-HA) is a high-added-value biopolymer with a wide range of applications in aesthetic and medical areas.
34597753	8	67	theme	SP	1296:1297	arg1	incorporation					1299:1311	SP incorporation	1296:1311	SP incorporation	1296:1311	Furthermore, SP incorporation also changed the coils' size regarding HA standards of molar mass 106,105 and 104 Da.
34605300	0	0	theme	peri-implantitis	125:140	arg1	repair					142:147	peri-implantitis repair	125:147	peri-implantitis repair	125:147	Preparation and characterization of antibacterial and anti-inflammatory hyaluronic acid-chitosan-dexamethasone hydrogels for peri-implantitis repair.
34605300	5	1	theme	in	739:740	arg1	repair					790:795	peri-implantitis repair	773:795	peri-implantitis repair	773:795	The in vivo hydrogels degradation and peri-implantitis repair were assessed in mice.
34605300	5	1	theme	in	739:740	arg1	hydrogels					747:755	The in vivo hydrogels	735:755	The in vivo hydrogels degradation and peri-implantitis repair	735:795	The in vivo hydrogels degradation and peri-implantitis repair were assessed in mice.
34605300	5	1	theme	in	739:740	arg1	degradation					757:767	degradation	757:767	degradation	757:767	The in vivo hydrogels degradation and peri-implantitis repair were assessed in mice.
34605300	9	2	with	hydrogels	1281:1289	arg1	function					1307:1314	integrated function	1296:1314	integrated function	1296:1314	The prepared HA-CT-DE composite hydrogels with integrated function is promising for the treatment of peri-implantitis.
34605300	8	3	theme	expression	1164:1173	arg1	levels					1175:1180	the expression levels	1160:1180	the expression levels of inflammation factor IL-1β, IL-6 and, TNF-α in peri-implantitis	1160:1246	It down-regulated the expression levels of inflammation factor IL-1β, IL-6 and, TNF-α in peri-implantitis.
34605300	3	4	theme	composite	567:575	arg1	system					587:592	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system	474:592	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system	474:592	Herein, an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis.
34605300	2	5	theme	peri-implantitis	420:435	arg1	repair					437:442	effective peri-implantitis repair	410:442	effective peri-implantitis repair	410:442	However, to continuously release antibacterial and anti-inflammatory drug in location in situ for effective peri-implantitis repair is still challenging.
34605300	6	6	theme	equilibrium	919:929	arg1	swelling					931:938	an equilibrium swelling	916:938	an equilibrium swelling of 18	916:944	The results showed that the prepared multifunctional hydrogels achieved sustained release, with an equilibrium swelling of 18, and promoted the growth against NIH-3T3 fibroblast cells.
34605300	2	7	theme	anti-inflammatory	363:379	arg1	drug					381:384	antibacterial and anti-inflammatory drug	345:384	antibacterial and anti-inflammatory drug	345:384	However, to continuously release antibacterial and anti-inflammatory drug in location in situ for effective peri-implantitis repair is still challenging.
34605300	9	8	theme	integrated	1296:1305	arg1	function					1307:1314	integrated function	1296:1314	integrated function	1296:1314	The prepared HA-CT-DE composite hydrogels with integrated function is promising for the treatment of peri-implantitis.
34605300	3	9	theme	repair	611:616	arg1	peri-implantitis					618:633	repair peri-implantitis	611:633	repair peri-implantitis	611:633	Herein, an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis.
34605300	2	10	theme	antibacterial	345:357	arg1	drug					381:384	antibacterial and anti-inflammatory drug	345:384	antibacterial and anti-inflammatory drug	345:384	However, to continuously release antibacterial and anti-inflammatory drug in location in situ for effective peri-implantitis repair is still challenging.
34605300	8	11	from	peri-implantitis	1231:1246	arg1	TNF-α					1222:1226	TNF-α	1222:1226	TNF-α	1222:1226	It down-regulated the expression levels of inflammation factor IL-1β, IL-6 and, TNF-α in peri-implantitis.
34605300	1	12	used	used	215:218	arg2	methods					169:175	Numerous treatment methods	150:175	Numerous treatment methods for peri-implantitis	150:196	Numerous treatment methods for peri-implantitis have been widely used including oral cleaning, traditional metal scraping means, or local antibiotic application.
34605300	3	13	theme	HA	549:550	arg1	CT					563:564	CT	563:564	CT	563:564	Herein, an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis.
34605300	3	13	theme	HA	549:550	arg1	-chitosan					552:560	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan	474:560	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system	474:592	Herein, an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis.
34605300	1	14	theme	oral	230:233	arg1	cleaning					235:242	oral cleaning	230:242	oral cleaning	230:242	Numerous treatment methods for peri-implantitis have been widely used including oral cleaning, traditional metal scraping means, or local antibiotic application.
34605300	3	15	theme	anti-inflammatory	477:493	arg1	CT					563:564	CT	563:564	CT	563:564	Herein, an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis.
34605300	3	15	theme	anti-inflammatory	477:493	arg1	-chitosan					552:560	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan	474:560	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system	474:592	Herein, an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis.
34605300	6	16	theme	fibroblast	987:996	arg1	cells					998:1002	NIH-3T3 fibroblast cells	979:1002	NIH-3T3 fibroblast cells	979:1002	The results showed that the prepared multifunctional hydrogels achieved sustained release, with an equilibrium swelling of 18, and promoted the growth against NIH-3T3 fibroblast cells.
34605300	8	17	theme	factor	1198:1203	arg1	levels					1175:1180	the expression levels	1160:1180	the expression levels of inflammation factor IL-1β, IL-6 and, TNF-α in peri-implantitis	1160:1246	It down-regulated the expression levels of inflammation factor IL-1β, IL-6 and, TNF-α in peri-implantitis.
34605300	7	18	theme	HA-CT-DE	1045:1052	arg1	hydrogels					1054:1062	HA-CT-DE hydrogels	1045:1062	HA-CT-DE hydrogels can inhibit methicillin-resistant Staphylococcus aureus and Escherichia coli	1045:1139	The in vitro antibacterial tests showed HA-CT-DE hydrogels can inhibit methicillin-resistant Staphylococcus aureus and Escherichia coli.
34605300	8	19	from	TNF-α	1222:1226	arg1	peri-implantitis					1231:1246	peri-implantitis	1231:1246	peri-implantitis	1231:1246	It down-regulated the expression levels of inflammation factor IL-1β, IL-6 and, TNF-α in peri-implantitis.
34605300	3	20	theme	-incorporated	518:530	arg1	CT					563:564	CT	563:564	CT	563:564	Herein, an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis.
34605300	3	20	theme	-incorporated	518:530	arg1	-chitosan					552:560	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan	474:560	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system	474:592	Herein, an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis.
34605300	5	21	dep	hydrogels	747:755	arg1	repair					790:795	peri-implantitis repair	773:795	peri-implantitis repair	773:795	The in vivo hydrogels degradation and peri-implantitis repair were assessed in mice.
34605300	5	21	dep	hydrogels	747:755	arg1	hydrogels					747:755	The in vivo hydrogels	735:755	The in vivo hydrogels degradation and peri-implantitis repair	735:795	The in vivo hydrogels degradation and peri-implantitis repair were assessed in mice.
34605300	5	21	dep	hydrogels	747:755	arg1	degradation					757:767	degradation	757:767	degradation	757:767	The in vivo hydrogels degradation and peri-implantitis repair were assessed in mice.
34605300	6	22	theme	NIH-3T3	979:985	arg1	cells					998:1002	NIH-3T3 fibroblast cells	979:1002	NIH-3T3 fibroblast cells	979:1002	The results showed that the prepared multifunctional hydrogels achieved sustained release, with an equilibrium swelling of 18, and promoted the growth against NIH-3T3 fibroblast cells.
34605300	8	23	theme	IL-6	1212:1215	arg1	levels					1175:1180	the expression levels	1160:1180	the expression levels of inflammation factor IL-1β, IL-6 and, TNF-α in peri-implantitis	1160:1246	It down-regulated the expression levels of inflammation factor IL-1β, IL-6 and, TNF-α in peri-implantitis.
34605300	6	24	theme	sustained	892:900	arg1	release					902:908	sustained release	892:908	sustained release	892:908	The results showed that the prepared multifunctional hydrogels achieved sustained release, with an equilibrium swelling of 18, and promoted the growth against NIH-3T3 fibroblast cells.
34605300	9	25	theme	prepared	1253:1260	arg1	hydrogels					1281:1289	The prepared HA-CT-DE composite hydrogels	1249:1289	The prepared HA-CT-DE composite hydrogels with integrated function	1249:1314	The prepared HA-CT-DE composite hydrogels with integrated function is promising for the treatment of peri-implantitis.
34605300	9	25	theme	prepared	1253:1260	arg1	promising					1319:1327	promising	1319:1327	promising	1319:1327	The prepared HA-CT-DE composite hydrogels with integrated function is promising for the treatment of peri-implantitis.
34605300	1	26	theme	traditional	245:255	arg1	means					272:276	traditional metal scraping means	245:276	traditional metal scraping means	245:276	Numerous treatment methods for peri-implantitis have been widely used including oral cleaning, traditional metal scraping means, or local antibiotic application.
34605300	5	27	dep	in	739:740	arg1	vivo					742:745	vivo	742:745	vivo	742:745	The in vivo hydrogels degradation and peri-implantitis repair were assessed in mice.
34605300	1	28	theme	Numerous	150:157	arg1	methods					169:175	Numerous treatment methods	150:175	Numerous treatment methods for peri-implantitis	150:196	Numerous treatment methods for peri-implantitis have been widely used including oral cleaning, traditional metal scraping means, or local antibiotic application.
34605300	1	29	theme	metal	257:261	arg1	means					272:276	traditional metal scraping means	245:276	traditional metal scraping means	245:276	Numerous treatment methods for peri-implantitis have been widely used including oral cleaning, traditional metal scraping means, or local antibiotic application.
34605300	4	30	theme	physicochemical	640:654	arg1	characterization					656:671	physicochemical characterization	640:671	physicochemical characterization	640:671	The physicochemical characterization and biocompatibility of the hydrogel were evaluated in vitro.
34605300	1	31	theme	treatment	159:167	arg1	methods					169:175	Numerous treatment methods	150:175	Numerous treatment methods for peri-implantitis	150:196	Numerous treatment methods for peri-implantitis have been widely used including oral cleaning, traditional metal scraping means, or local antibiotic application.
34605300	1	32	theme	scraping	263:270	arg1	means					272:276	traditional metal scraping means	245:276	traditional metal scraping means	245:276	Numerous treatment methods for peri-implantitis have been widely used including oral cleaning, traditional metal scraping means, or local antibiotic application.
34605300	3	33	theme	drug	495:498	arg1	CT					563:564	CT	563:564	CT	563:564	Herein, an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis.
34605300	3	33	theme	drug	495:498	arg1	-chitosan					552:560	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan	474:560	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system	474:592	Herein, an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis.
34605300	3	34	theme	DE	515:516	arg1	CT					563:564	CT	563:564	CT	563:564	Herein, an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis.
34605300	3	34	theme	DE	515:516	arg1	-chitosan					552:560	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan	474:560	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system	474:592	Herein, an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis.
34605300	3	35	theme	hyaluronic	532:541	arg1	CT					563:564	CT	563:564	CT	563:564	Herein, an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis.
34605300	3	35	theme	hyaluronic	532:541	arg1	-chitosan					552:560	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan	474:560	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system	474:592	Herein, an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis.
34605300	0	36	theme	antibacterial	36:48	arg1	acid-chitosan-dexamethasone					83:109	antibacterial and anti-inflammatory hyaluronic acid-chitosan-dexamethasone	36:109	antibacterial and anti-inflammatory hyaluronic acid-chitosan-dexamethasone	36:109	Preparation and characterization of antibacterial and anti-inflammatory hyaluronic acid-chitosan-dexamethasone hydrogels for peri-implantitis repair.
34605300	3	37	theme	dexamethasone	500:512	arg1	CT					563:564	CT	563:564	CT	563:564	Herein, an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis.
34605300	3	37	theme	dexamethasone	500:512	arg1	-chitosan					552:560	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan	474:560	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system	474:592	Herein, an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis.
34605300	9	38	theme	HA-CT-DE	1262:1269	arg1	hydrogels					1281:1289	The prepared HA-CT-DE composite hydrogels	1249:1289	The prepared HA-CT-DE composite hydrogels with integrated function	1249:1314	The prepared HA-CT-DE composite hydrogels with integrated function is promising for the treatment of peri-implantitis.
34605300	9	38	theme	HA-CT-DE	1262:1269	arg1	promising					1319:1327	promising	1319:1327	promising	1319:1327	The prepared HA-CT-DE composite hydrogels with integrated function is promising for the treatment of peri-implantitis.
34605300	3	39	theme	acid	543:546	arg1	CT					563:564	CT	563:564	CT	563:564	Herein, an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis.
34605300	3	39	theme	acid	543:546	arg1	-chitosan					552:560	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan	474:560	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system	474:592	Herein, an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis.
34605300	0	40	theme	hyaluronic	72:81	arg1	acid-chitosan-dexamethasone					83:109	antibacterial and anti-inflammatory hyaluronic acid-chitosan-dexamethasone	36:109	antibacterial and anti-inflammatory hyaluronic acid-chitosan-dexamethasone	36:109	Preparation and characterization of antibacterial and anti-inflammatory hyaluronic acid-chitosan-dexamethasone hydrogels for peri-implantitis repair.
34605300	4	41	dep	characterization	656:671	arg1	The					636:638	The	636:638	The	636:638	The physicochemical characterization and biocompatibility of the hydrogel were evaluated in vitro.
34605300	6	42	theme	multifunctional	857:871	arg1	hydrogels					873:881	the prepared multifunctional hydrogels	844:881	the prepared multifunctional hydrogels	844:881	The results showed that the prepared multifunctional hydrogels achieved sustained release, with an equilibrium swelling of 18, and promoted the growth against NIH-3T3 fibroblast cells.
34605300	2	43	theme	effective	410:418	arg1	repair					437:442	effective peri-implantitis repair	410:442	effective peri-implantitis repair	410:442	However, to continuously release antibacterial and anti-inflammatory drug in location in situ for effective peri-implantitis repair is still challenging.
34605300	0	44	theme	anti-inflammatory	54:70	arg1	acid-chitosan-dexamethasone					83:109	antibacterial and anti-inflammatory hyaluronic acid-chitosan-dexamethasone	36:109	antibacterial and anti-inflammatory hyaluronic acid-chitosan-dexamethasone	36:109	Preparation and characterization of antibacterial and anti-inflammatory hyaluronic acid-chitosan-dexamethasone hydrogels for peri-implantitis repair.
34605300	5	45	theme	peri-implantitis	773:788	arg1	repair					790:795	peri-implantitis repair	773:795	peri-implantitis repair	773:795	The in vivo hydrogels degradation and peri-implantitis repair were assessed in mice.
34605300	5	45	theme	peri-implantitis	773:788	arg1	hydrogels					747:755	The in vivo hydrogels	735:755	The in vivo hydrogels degradation and peri-implantitis repair	735:795	The in vivo hydrogels degradation and peri-implantitis repair were assessed in mice.
34605300	6	46	theme	prepared	848:855	arg1	hydrogels					873:881	the prepared multifunctional hydrogels	844:881	the prepared multifunctional hydrogels	844:881	The results showed that the prepared multifunctional hydrogels achieved sustained release, with an equilibrium swelling of 18, and promoted the growth against NIH-3T3 fibroblast cells.
34605300	8	47	theme	inflammation	1185:1196	arg1	factor					1198:1203	inflammation factor IL-1β	1185:1209	inflammation factor IL-1β	1185:1209	It down-regulated the expression levels of inflammation factor IL-1β, IL-6 and, TNF-α in peri-implantitis.
34605300	9	48	theme	peri-implantitis	1350:1365	arg1	treatment					1337:1345	the treatment	1333:1345	the treatment of peri-implantitis	1333:1365	The prepared HA-CT-DE composite hydrogels with integrated function is promising for the treatment of peri-implantitis.
34605300	7	49	dep	in	1009:1010	arg1	vitro					1012:1016	vitro	1012:1016	vitro	1012:1016	The in vitro antibacterial tests showed HA-CT-DE hydrogels can inhibit methicillin-resistant Staphylococcus aureus and Escherichia coli.
34605300	7	50	theme	antibacterial	1018:1030	arg1	tests					1032:1036	The in vitro antibacterial tests	1005:1036	The in vitro antibacterial tests	1005:1036	The in vitro antibacterial tests showed HA-CT-DE hydrogels can inhibit methicillin-resistant Staphylococcus aureus and Escherichia coli.
34605300	4	51	theme	hydrogel	701:708	arg1	biocompatibility					677:692	biocompatibility	677:692	biocompatibility	677:692	The physicochemical characterization and biocompatibility of the hydrogel were evaluated in vitro.
34605300	4	51	theme	hydrogel	701:708	arg1	characterization					656:671	physicochemical characterization	640:671	physicochemical characterization	640:671	The physicochemical characterization and biocompatibility of the hydrogel were evaluated in vitro.
34605300	6	52	theme	18	943:944	arg1	swelling					931:938	an equilibrium swelling	916:938	an equilibrium swelling of 18	916:944	The results showed that the prepared multifunctional hydrogels achieved sustained release, with an equilibrium swelling of 18, and promoted the growth against NIH-3T3 fibroblast cells.
34605300	9	53	theme	composite	1271:1279	arg1	hydrogels					1281:1289	The prepared HA-CT-DE composite hydrogels	1249:1289	The prepared HA-CT-DE composite hydrogels with integrated function	1249:1314	The prepared HA-CT-DE composite hydrogels with integrated function is promising for the treatment of peri-implantitis.
34605300	9	53	theme	composite	1271:1279	arg1	promising					1319:1327	promising	1319:1327	promising	1319:1327	The prepared HA-CT-DE composite hydrogels with integrated function is promising for the treatment of peri-implantitis.
34605300	3	54	theme	-chitosan	552:560	arg1	system					587:592	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system	474:592	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system	474:592	Herein, an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis.
34605300	1	55	theme	local	282:286	arg1	application					299:309	local antibiotic application	282:309	local antibiotic application	282:309	Numerous treatment methods for peri-implantitis have been widely used including oral cleaning, traditional metal scraping means, or local antibiotic application.
34605300	7	56	dep	hydrogels	1054:1062	arg1	inhibit					1068:1074	inhibit	1068:1074	can inhibit methicillin-resistant Staphylococcus aureus and Escherichia coli	1064:1139	The in vitro antibacterial tests showed HA-CT-DE hydrogels can inhibit methicillin-resistant Staphylococcus aureus and Escherichia coli.
34605300	3	57	theme	hydrogels	577:585	arg1	system					587:592	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system	474:592	an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system	474:592	Herein, an anti-inflammatory drug dexamethasone (DE)-incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis.
34605300	7	58	theme	in	1009:1010	arg1	tests					1032:1036	The in vitro antibacterial tests	1005:1036	The in vitro antibacterial tests	1005:1036	The in vitro antibacterial tests showed HA-CT-DE hydrogels can inhibit methicillin-resistant Staphylococcus aureus and Escherichia coli.
34605300	1	59	theme	antibiotic	288:297	arg1	application					299:309	local antibiotic application	282:309	local antibiotic application	282:309	Numerous treatment methods for peri-implantitis have been widely used including oral cleaning, traditional metal scraping means, or local antibiotic application.
34605300	0	60	theme	acid-chitosan-dexamethasone	83:109	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of antibacterial and anti-inflammatory hyaluronic acid-chitosan-dexamethasone hydrogels for peri-implantitis repair.
34605300	0	60	theme	acid-chitosan-dexamethasone	83:109	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of antibacterial and anti-inflammatory hyaluronic acid-chitosan-dexamethasone hydrogels for peri-implantitis repair.
34605300	8	61	theme	TNF-α	1222:1226	arg1	factor					1198:1203	inflammation factor IL-1β	1185:1209	inflammation factor IL-1β	1185:1209	It down-regulated the expression levels of inflammation factor IL-1β, IL-6 and, TNF-α in peri-implantitis.
34617273	9	0	theme	Ca/P	1716:1719	arg1	ratio					1728:1732	calcium/phosphorus (Ca/P) molar ratio	1696:1732	calcium/phosphorus (Ca/P) molar ratio	1696:1732	RESULTS Synthesized hydroxyapatite (HAp) comprised crystals ranging from 20 to 50 nm (HAnp) with spherulite morphology and calcium/phosphorus (Ca/P) molar ratio of 1.67.
34617273	15	1	from	day	2548:2550	arg1	intensity					2512:2520	Mean fluorescence intensity	2494:2520	Mean fluorescence intensity of the VEGF expression at day 21 (32.5)	2494:2560	Mean fluorescence intensity of the VEGF expression at day 21 (32.5) was also significantly higher (p < .01) than at day 7 (12.54).
34617273	16	2	theme	cell	2689:2692	arg1	viability/proliferation					2694:2716	increased cell viability/proliferation	2679:2716	increased cell viability/proliferation	2679:2716	CONCLUSION hDPSCs on 1:5 HAnp-CMC scaffolds displayed increased cell viability/proliferation and enhanced DSPP as well as VEGF expressions.
34617273	10	3	theme	scaffolds	1773:1781	arg1	ultrastructure					1747:1760	The ultrastructure	1743:1760	The ultrastructure of all the scaffolds	1743:1781	The ultrastructure of all the scaffolds revealed a highly interconnected porous microstructure, whilst the chemical characterization displayed specific functional groups of both HAnp and CMC.
34617273	2	4	attach	derived	410:416	arg2	Nano-hydroxyapatite					386:404	METHODOLOGY Nano-hydroxyapatite	374:404	METHODOLOGY Nano-hydroxyapatite	374:404	METHODOLOGY Nano-hydroxyapatite was derived from eggshells using a simple combustion method and CMC was prepared from chitosan through a chemical route.
34617273	2	4	attach	derived	410:416	arg1	eggshells					423:431	eggshells	423:431	eggshells using a simple combustion method	423:464	METHODOLOGY Nano-hydroxyapatite was derived from eggshells using a simple combustion method and CMC was prepared from chitosan through a chemical route.
34617273	9	5	theme	molar	1722:1726	arg1	ratio					1728:1732	calcium/phosphorus (Ca/P) molar ratio	1696:1732	calcium/phosphorus (Ca/P) molar ratio	1696:1732	RESULTS Synthesized hydroxyapatite (HAp) comprised crystals ranging from 20 to 50 nm (HAnp) with spherulite morphology and calcium/phosphorus (Ca/P) molar ratio of 1.67.
34617273	11	6	theme	crystal	2047:2053	arg1	formation					2055:2063	an apatite-like crystal formation	2031:2063	an apatite-like crystal formation on the surface	2031:2078	In vitro bioactivity assessment confirmed the biomineralization potential of all scaffolds with an apatite-like crystal formation on the surface.
34617273	12	7	theme	favourable	2109:2118	arg1	size					2125:2128	a favourable pore size	2107:2128	a favourable pore size (60-180 µm) that was suitable for cell seeding and was chosen for further experiments	2107:2214	The 1:5 HAnp-CMC revealed a favourable pore size (60-180 µm) that was suitable for cell seeding and was chosen for further experiments.
34617273	12	7	theme	favourable	2109:2118	arg1	suitable					2151:2158	suitable	2151:2158	suitable	2151:2158	The 1:5 HAnp-CMC revealed a favourable pore size (60-180 µm) that was suitable for cell seeding and was chosen for further experiments.
34617273	12	7	theme	favourable	2109:2118	arg1	60-180 µm					2131:2139	60-180 µm	2131:2139	60-180 µm	2131:2139	The 1:5 HAnp-CMC revealed a favourable pore size (60-180 µm) that was suitable for cell seeding and was chosen for further experiments.
34617273	12	8	theme	cell	2164:2167	arg1	seeding					2169:2175	cell seeding	2164:2175	cell seeding	2164:2175	The 1:5 HAnp-CMC revealed a favourable pore size (60-180 µm) that was suitable for cell seeding and was chosen for further experiments.
34617273	6	9	theme	polymerase	1270:1279	arg1	reaction					1287:1294	real-time quantitative polymerase chain reaction	1247:1294	real-time quantitative polymerase chain reaction	1247:1294	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	6	10	dep	assay	1240:1244	arg1	methods					1315:1321	methods	1315:1321	methods	1315:1321	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	10	11	theme	interconnected	1801:1814	arg1	microstructure					1823:1836	a highly interconnected porous microstructure	1792:1836	a highly interconnected porous microstructure	1792:1836	The ultrastructure of all the scaffolds revealed a highly interconnected porous microstructure, whilst the chemical characterization displayed specific functional groups of both HAnp and CMC.
34617273	1	12	theme	scaffolds	225:233	arg1	abilities					178:186	odontogenic differentiation abilities	150:186	odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs)	150:371	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	6	13	theme	real-time	1247:1255	arg1	reaction					1287:1294	real-time quantitative polymerase chain reaction	1247:1294	real-time quantitative polymerase chain reaction	1247:1294	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	4	14	theme	HAnp-CMC	676:683	arg1	scaffolds					685:693	HAnp-CMC scaffolds	676:693	HAnp-CMC scaffolds	676:693	HAnp-CMC scaffolds were characterized using high-resolution scanning electron microscopy combined with energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction methods.
34617273	10	15	theme	chemical	1850:1857	arg1	characterization					1859:1874	the chemical characterization	1846:1874	the chemical characterization	1846:1874	The ultrastructure of all the scaffolds revealed a highly interconnected porous microstructure, whilst the chemical characterization displayed specific functional groups of both HAnp and CMC.
34617273	12	16	theme	1:5	2085:2087	arg1	HAnp-CMC					2089:2096	The 1:5 HAnp-CMC	2081:2096	The 1:5 HAnp-CMC	2081:2096	The 1:5 HAnp-CMC revealed a favourable pore size (60-180 µm) that was suitable for cell seeding and was chosen for further experiments.
34617273	7	17	theme	post	1497:1500	arg1	test					1506:1509	appropriate post hoc test	1485:1509	appropriate post hoc test (Dunn)	1485:1516	For intergroup and intragroup comparisons, Kruskal-Wallis and Friedman tests were employed, respectively, followed by appropriate post hoc test (Dunn).
34617273	7	17	theme	post	1497:1500	arg1	Dunn					1512:1515	Dunn	1512:1515	Dunn	1512:1515	For intergroup and intragroup comparisons, Kruskal-Wallis and Friedman tests were employed, respectively, followed by appropriate post hoc test (Dunn).
34617273	11	18	from	formation	2055:2063	arg1	surface					2072:2078	the surface	2068:2078	the surface	2068:2078	In vitro bioactivity assessment confirmed the biomineralization potential of all scaffolds with an apatite-like crystal formation on the surface.
34617273	16	19	theme	increased	2679:2687	arg1	viability/proliferation					2694:2716	increased cell viability/proliferation	2679:2716	increased cell viability/proliferation	2679:2716	CONCLUSION hDPSCs on 1:5 HAnp-CMC scaffolds displayed increased cell viability/proliferation and enhanced DSPP as well as VEGF expressions.
34617273	7	20	dep	employed	1449:1456	arg1	followed					1473:1480	followed	1473:1480	followed by appropriate post hoc test (Dunn)	1473:1516	For intergroup and intragroup comparisons, Kruskal-Wallis and Friedman tests were employed, respectively, followed by appropriate post hoc test (Dunn).
34617273	13	21	dep	HAnp-CMC	2273:2280	arg1	day					2290:2292	21st day	2285:2292	21st day	2285:2292	Cell viability/proliferation rates of hDPSCs loaded 1:5 HAnp-CMC at 21st day was significantly greater than that at 7th day (p < .05).
34617273	12	22	theme	further	2196:2202	arg1	experiments					2204:2214	further experiments	2196:2214	further experiments	2196:2214	The 1:5 HAnp-CMC revealed a favourable pore size (60-180 µm) that was suitable for cell seeding and was chosen for further experiments.
34617273	13	23	theme	Cell	2217:2220	arg1	rates					2246:2250	Cell viability/proliferation rates	2217:2250	Cell viability/proliferation rates of hDPSCs loaded 1:5 HAnp-CMC at 21st day	2217:2292	Cell viability/proliferation rates of hDPSCs loaded 1:5 HAnp-CMC at 21st day was significantly greater than that at 7th day (p < .05).
34617273	10	24	theme	functional	1895:1904	arg1	CMC					1930:1932	CMC	1930:1932	CMC	1930:1932	The ultrastructure of all the scaffolds revealed a highly interconnected porous microstructure, whilst the chemical characterization displayed specific functional groups of both HAnp and CMC.
34617273	10	24	theme	functional	1895:1904	arg1	groups					1906:1911	specific functional groups	1886:1911	specific functional groups of both HAnp and CMC	1886:1932	The ultrastructure of all the scaffolds revealed a highly interconnected porous microstructure, whilst the chemical characterization displayed specific functional groups of both HAnp and CMC.
34617273	10	24	theme	functional	1895:1904	arg1	HAnp					1921:1924	HAnp	1921:1924	HAnp	1921:1924	The ultrastructure of all the scaffolds revealed a highly interconnected porous microstructure, whilst the chemical characterization displayed specific functional groups of both HAnp and CMC.
34617273	1	25	theme	human	335:339	arg1	cells					358:362	cultured human dental pulp stem cells	326:362	cultured human dental pulp stem cells (hDPSCs)	326:371	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	1	25	theme	human	335:339	arg1	hDPSCs					365:370	hDPSCs	365:370	hDPSCs	365:370	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	4	26	theme	scanning	736:743	arg1	Fourier					817:823	Fourier	817:823	Fourier transform infrared spectroscopy and X-ray diffraction methods	817:885	HAnp-CMC scaffolds were characterized using high-resolution scanning electron microscopy combined with energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction methods.
34617273	4	26	theme	scanning	736:743	arg1	microscopy					754:763	high-resolution scanning electron microscopy	720:763	high-resolution scanning electron microscopy combined with energy-dispersive X-ray spectroscopy	720:814	HAnp-CMC scaffolds were characterized using high-resolution scanning electron microscopy combined with energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction methods.
34617273	15	27	theme	fluorescence	2499:2510	arg1	intensity					2512:2520	Mean fluorescence intensity	2494:2520	Mean fluorescence intensity of the VEGF expression at day 21 (32.5)	2494:2560	Mean fluorescence intensity of the VEGF expression at day 21 (32.5) was also significantly higher (p < .01) than at day 7 (12.54).
34617273	10	28	theme	HAnp	1921:1924	arg1	CMC					1930:1932	CMC	1930:1932	CMC	1930:1932	The ultrastructure of all the scaffolds revealed a highly interconnected porous microstructure, whilst the chemical characterization displayed specific functional groups of both HAnp and CMC.
34617273	10	28	theme	HAnp	1921:1924	arg1	groups					1906:1911	specific functional groups	1886:1911	specific functional groups of both HAnp and CMC	1886:1932	The ultrastructure of all the scaffolds revealed a highly interconnected porous microstructure, whilst the chemical characterization displayed specific functional groups of both HAnp and CMC.
34617273	10	28	theme	HAnp	1921:1924	arg1	HAnp					1921:1924	HAnp	1921:1924	HAnp	1921:1924	The ultrastructure of all the scaffolds revealed a highly interconnected porous microstructure, whilst the chemical characterization displayed specific functional groups of both HAnp and CMC.
34617273	5	29	from	interaction	938:948	arg1	fluid					968:972	simulated body fluid	953:972	simulated body fluid for 21 days	953:984	In vitro bioactivity was determined following the interaction in simulated body fluid for 21 days.
34617273	1	30	theme	differentiation	162:176	arg1	abilities					178:186	odontogenic differentiation abilities	150:186	odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs)	150:371	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	6	31	theme	flow	1300:1303	arg1	cytometry					1305:1313	flow cytometry	1300:1313	flow cytometry	1300:1313	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	0	32	theme	dentine	86:92	arg1	regeneration					94:105	dentine regeneration	86:105	dentine regeneration	86:105	Eggshell derived nano-hydroxyapatite incorporated carboxymethyl chitosan scaffold for dentine regeneration: A laboratory investigation.
34617273	5	33	theme	In	888:889	arg1	bioactivity					897:907	In vitro bioactivity	888:907	In vitro bioactivity	888:907	In vitro bioactivity was determined following the interaction in simulated body fluid for 21 days.
34617273	5	34	theme	body	963:966	arg1	fluid					968:972	simulated body fluid	953:972	simulated body fluid for 21 days	953:984	In vitro bioactivity was determined following the interaction in simulated body fluid for 21 days.
34617273	14	35	theme	DSPP	2388:2391	arg1	expression					2393:2402	DSPP expression	2388:2402	DSPP expression	2388:2402	The mean relative quantification of DSPP expression by the scaffold was significantly higher (p < .05) on day 21 (3.16) than on day 7 (1.67).
34617273	1	36	theme	biomineralizable	198:213	arg1	scaffolds					225:233	porous biomineralizable composite scaffolds	191:233	porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC)	191:321	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	1	37	link	derived	255:261	arg1	nano-hydroxyapatite					263:281	eggshell derived nano-hydroxyapatite	246:281	eggshell derived nano-hydroxyapatite (HAnp)	246:288	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	1	37	link	derived	255:261	arg1	HAnp					284:287	HAnp	284:287	HAnp	284:287	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	0	38	theme	laboratory	110:119	arg1	investigation					121:133	A laboratory investigation	108:133	A laboratory investigation	108:133	Eggshell derived nano-hydroxyapatite incorporated carboxymethyl chitosan scaffold for dentine regeneration: A laboratory investigation.
34617273	1	39	from	abilities	178:186	arg1	cells					358:362	cultured human dental pulp stem cells	326:362	cultured human dental pulp stem cells (hDPSCs)	326:371	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	1	39	from	abilities	178:186	arg1	hDPSCs					365:370	hDPSCs	365:370	hDPSCs	365:370	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	6	40	theme	cell	1049:1052	arg1	viability/proliferation					1054:1076	cell viability/proliferation	1049:1076	cell viability/proliferation	1049:1076	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	11	41	theme	apatite-like	2034:2045	arg1	formation					2055:2063	an apatite-like crystal formation	2031:2063	an apatite-like crystal formation on the surface	2031:2078	In vitro bioactivity assessment confirmed the biomineralization potential of all scaffolds with an apatite-like crystal formation on the surface.
34617273	14	42	theme	mean	2356:2359	arg1	quantification					2370:2383	The mean relative quantification	2352:2383	The mean relative quantification of DSPP expression by the scaffold	2352:2418	The mean relative quantification of DSPP expression by the scaffold was significantly higher (p < .05) on day 21 (3.16) than on day 7 (1.67).
34617273	14	42	theme	mean	2356:2359	arg1	higher					2438:2443	higher	2438:2443	higher	2438:2443	The mean relative quantification of DSPP expression by the scaffold was significantly higher (p < .05) on day 21 (3.16) than on day 7 (1.67).
34617273	2	43	theme	combustion	448:457	arg1	method					459:464	a simple combustion method	439:464	a simple combustion method	439:464	METHODOLOGY Nano-hydroxyapatite was derived from eggshells using a simple combustion method and CMC was prepared from chitosan through a chemical route.
34617273	3	44	dep	HAnp-CMC	551:558	arg1	%					601:601	0:5, 5:0, 1:5, 2:5, 3:5, 4:5 and 1:1 w/w%	561:601	0:5, 5:0, 1:5, 2:5, 3:5, 4:5 and 1:1 w/w%	561:601	Several compositions of HAnp-CMC (0:5, 5:0, 1:5, 2:5, 3:5, 4:5 and 1:1 w/w%) scaffolds were prepared by magnetic stirring and freeze-drying methods.
34617273	13	45	theme	7th	2333:2335	arg1	p < .05					2342:2348	p < .05	2342:2348	p < .05	2342:2348	Cell viability/proliferation rates of hDPSCs loaded 1:5 HAnp-CMC at 21st day was significantly greater than that at 7th day (p < .05).
34617273	13	45	theme	7th	2333:2335	arg1	day					2337:2339	7th day	2333:2339	7th day (p < .05)	2333:2349	Cell viability/proliferation rates of hDPSCs loaded 1:5 HAnp-CMC at 21st day was significantly greater than that at 7th day (p < .05).
34617273	17	46	contain	has	2792:2794	arg1	composite					2782:2790	The 1:5 HAnp-CMC composite	2765:2790	The 1:5 HAnp-CMC composite	2765:2790	The 1:5 HAnp-CMC composite has the potential to serve as a promising scaffold for dentine regeneration.
34617273	17	46	contain	has	2792:2794	arg2	potential					2800:2808	the potential to serve as a promising scaffold for dentine regeneration	2796:2866	the potential to serve as a promising scaffold for dentine regeneration	2796:2866	The 1:5 HAnp-CMC composite has the potential to serve as a promising scaffold for dentine regeneration.
34617273	11	47	theme	biomineralization	1981:1997	arg1	potential					1999:2007	the biomineralization potential	1977:2007	the biomineralization potential of all scaffolds	1977:2024	In vitro bioactivity assessment confirmed the biomineralization potential of all scaffolds with an apatite-like crystal formation on the surface.
34617273	1	48	theme	cultured	326:333	arg1	cells					358:362	cultured human dental pulp stem cells	326:362	cultured human dental pulp stem cells (hDPSCs)	326:371	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	1	48	theme	cultured	326:333	arg1	hDPSCs					365:370	hDPSCs	365:370	hDPSCs	365:370	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	15	49	theme	VEGF	2529:2532	arg1	expression					2534:2543	the VEGF expression	2525:2543	the VEGF expression	2525:2543	Mean fluorescence intensity of the VEGF expression at day 21 (32.5) was also significantly higher (p < .01) than at day 7 (12.54).
34617273	4	50	theme	X-ray	797:801	arg1	spectroscopy					803:814	energy-dispersive X-ray spectroscopy	779:814	energy-dispersive X-ray spectroscopy	779:814	HAnp-CMC scaffolds were characterized using high-resolution scanning electron microscopy combined with energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction methods.
34617273	0	51	dep	derived	9:15	arg1	investigation					121:133	A laboratory investigation	108:133	A laboratory investigation	108:133	Eggshell derived nano-hydroxyapatite incorporated carboxymethyl chitosan scaffold for dentine regeneration: A laboratory investigation.
34617273	0	52	theme	nano-hydroxyapatite	17:35	arg1	scaffold					73:80	nano-hydroxyapatite incorporated carboxymethyl chitosan scaffold	17:80	nano-hydroxyapatite incorporated carboxymethyl chitosan scaffold for dentine regeneration	17:105	Eggshell derived nano-hydroxyapatite incorporated carboxymethyl chitosan scaffold for dentine regeneration: A laboratory investigation.
34617273	1	53	theme	dental	341:346	arg1	cells					358:362	cultured human dental pulp stem cells	326:362	cultured human dental pulp stem cells (hDPSCs)	326:371	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	1	53	theme	dental	341:346	arg1	hDPSCs					365:370	hDPSCs	365:370	hDPSCs	365:370	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	6	54	theme	endothelial	1127:1137	arg1	VEGF					1154:1157	VEGF	1154:1157	VEGF	1154:1157	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	6	54	theme	endothelial	1127:1137	arg1	factor					1146:1151	vascular endothelial growth factor	1118:1151	vascular endothelial growth factor (VEGF)	1118:1158	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	17	55	theme	HAnp-CMC	2773:2780	arg1	composite					2782:2790	The 1:5 HAnp-CMC composite	2765:2790	The 1:5 HAnp-CMC composite	2765:2790	The 1:5 HAnp-CMC composite has the potential to serve as a promising scaffold for dentine regeneration.
34617273	16	56	theme	VEGF	2747:2750	arg1	expressions					2752:2762	VEGF expressions	2747:2762	DSPP as well as VEGF expressions	2731:2762	CONCLUSION hDPSCs on 1:5 HAnp-CMC scaffolds displayed increased cell viability/proliferation and enhanced DSPP as well as VEGF expressions.
34617273	11	57	theme	scaffolds	2016:2024	arg1	potential					1999:2007	the biomineralization potential	1977:2007	the biomineralization potential of all scaffolds	1977:2024	In vitro bioactivity assessment confirmed the biomineralization potential of all scaffolds with an apatite-like crystal formation on the surface.
34617273	0	58	theme	carboxymethyl	50:62	arg1	scaffold					73:80	nano-hydroxyapatite incorporated carboxymethyl chitosan scaffold	17:80	nano-hydroxyapatite incorporated carboxymethyl chitosan scaffold for dentine regeneration	17:105	Eggshell derived nano-hydroxyapatite incorporated carboxymethyl chitosan scaffold for dentine regeneration: A laboratory investigation.
34617273	1	59	theme	stem	353:356	arg1	cells					358:362	cultured human dental pulp stem cells	326:362	cultured human dental pulp stem cells (hDPSCs)	326:371	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	1	59	theme	stem	353:356	arg1	hDPSCs					365:370	hDPSCs	365:370	hDPSCs	365:370	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	7	60	theme	intragroup	1386:1395	arg1	comparisons					1397:1407	intergroup and intragroup comparisons	1371:1407	comparisons	1397:1407	For intergroup and intragroup comparisons, Kruskal-Wallis and Friedman tests were employed, respectively, followed by appropriate post hoc test (Dunn).
34617273	2	61	theme	chemical	511:518	arg1	route					520:524	a chemical route	509:524	a chemical route	509:524	METHODOLOGY Nano-hydroxyapatite was derived from eggshells using a simple combustion method and CMC was prepared from chitosan through a chemical route.
34617273	10	62	theme	CMC	1930:1932	arg1	CMC					1930:1932	CMC	1930:1932	CMC	1930:1932	The ultrastructure of all the scaffolds revealed a highly interconnected porous microstructure, whilst the chemical characterization displayed specific functional groups of both HAnp and CMC.
34617273	10	62	theme	CMC	1930:1932	arg1	groups					1906:1911	specific functional groups	1886:1911	specific functional groups of both HAnp and CMC	1886:1932	The ultrastructure of all the scaffolds revealed a highly interconnected porous microstructure, whilst the chemical characterization displayed specific functional groups of both HAnp and CMC.
34617273	10	62	theme	CMC	1930:1932	arg1	HAnp					1921:1924	HAnp	1921:1924	HAnp	1921:1924	The ultrastructure of all the scaffolds revealed a highly interconnected porous microstructure, whilst the chemical characterization displayed specific functional groups of both HAnp and CMC.
34617273	11	63	theme	In	1935:1936	arg1	assessment					1956:1965	In vitro bioactivity assessment	1935:1965	In vitro bioactivity assessment	1935:1965	In vitro bioactivity assessment confirmed the biomineralization potential of all scaffolds with an apatite-like crystal formation on the surface.
34617273	9	64	theme	spherulite	1670:1679	arg1	morphology					1681:1690	spherulite morphology	1670:1690	spherulite morphology	1670:1690	RESULTS Synthesized hydroxyapatite (HAp) comprised crystals ranging from 20 to 50 nm (HAnp) with spherulite morphology and calcium/phosphorus (Ca/P) molar ratio of 1.67.
34617273	6	65	theme	-2,5-diphenyl	1206:1218	arg1	bromide					1232:1238	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide	1178:1238	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay	1178:1244	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	3	66	theme	scaffolds	604:612	arg1	compositions					535:546	Several compositions	527:546	Several compositions of HAnp-CMC (0:5, 5:0, 1:5, 2:5, 3:5, 4:5 and 1:1 w/w%) scaffolds	527:612	Several compositions of HAnp-CMC (0:5, 5:0, 1:5, 2:5, 3:5, 4:5 and 1:1 w/w%) scaffolds were prepared by magnetic stirring and freeze-drying methods.
34617273	3	67	theme	Several	527:533	arg1	compositions					535:546	Several compositions	527:546	Several compositions of HAnp-CMC (0:5, 5:0, 1:5, 2:5, 3:5, 4:5 and 1:1 w/w%) scaffolds	527:612	Several compositions of HAnp-CMC (0:5, 5:0, 1:5, 2:5, 3:5, 4:5 and 1:1 w/w%) scaffolds were prepared by magnetic stirring and freeze-drying methods.
34617273	6	68	theme	4,5-dimethylthiazol-2-yl	1181:1204	arg1	bromide					1232:1238	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide	1178:1238	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay	1178:1244	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	1	69	dep	derived	255:261	arg1	eggshell					246:253	eggshell	246:253	eggshell	246:253	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	1	70	theme	carboxymethyl	294:306	arg1	CMC					318:320	CMC	318:320	CMC	318:320	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	1	70	theme	carboxymethyl	294:306	arg1	chitosan					308:315	carboxymethyl chitosan	294:315	carboxymethyl chitosan (CMC)	294:321	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	12	71	theme	pore	2120:2123	arg1	size					2125:2128	a favourable pore size	2107:2128	a favourable pore size (60-180 µm) that was suitable for cell seeding and was chosen for further experiments	2107:2214	The 1:5 HAnp-CMC revealed a favourable pore size (60-180 µm) that was suitable for cell seeding and was chosen for further experiments.
34617273	12	71	theme	pore	2120:2123	arg1	suitable					2151:2158	suitable	2151:2158	suitable	2151:2158	The 1:5 HAnp-CMC revealed a favourable pore size (60-180 µm) that was suitable for cell seeding and was chosen for further experiments.
34617273	12	71	theme	pore	2120:2123	arg1	60-180 µm					2131:2139	60-180 µm	2131:2139	60-180 µm	2131:2139	The 1:5 HAnp-CMC revealed a favourable pore size (60-180 µm) that was suitable for cell seeding and was chosen for further experiments.
34617273	6	72	theme	optimized	991:999	arg1	composite					1001:1009	The optimized composite	987:1009	The optimized composite	987:1009	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	7	73	theme	intergroup	1371:1380	arg1	comparisons					1397:1407	intergroup and intragroup comparisons	1371:1407	comparisons	1397:1407	For intergroup and intragroup comparisons, Kruskal-Wallis and Friedman tests were employed, respectively, followed by appropriate post hoc test (Dunn).
34617273	11	74	theme	bioactivity	1944:1954	arg1	assessment					1956:1965	In vitro bioactivity assessment	1935:1965	In vitro bioactivity assessment	1935:1965	In vitro bioactivity assessment confirmed the biomineralization potential of all scaffolds with an apatite-like crystal formation on the surface.
34617273	5	75	dep	In	888:889	arg1	vitro					891:895	vitro	891:895	vitro	891:895	In vitro bioactivity was determined following the interaction in simulated body fluid for 21 days.
34617273	3	76	theme	magnetic	631:638	arg1	methods					667:673	magnetic stirring and freeze-drying methods	631:673	magnetic stirring and freeze-drying methods	631:673	Several compositions of HAnp-CMC (0:5, 5:0, 1:5, 2:5, 3:5, 4:5 and 1:1 w/w%) scaffolds were prepared by magnetic stirring and freeze-drying methods.
34617273	13	77	theme	hDPSCs	2255:2260	arg1	rates					2246:2250	Cell viability/proliferation rates	2217:2250	Cell viability/proliferation rates of hDPSCs loaded 1:5 HAnp-CMC at 21st day	2217:2292	Cell viability/proliferation rates of hDPSCs loaded 1:5 HAnp-CMC at 21st day was significantly greater than that at 7th day (p < .05).
34617273	4	78	dep	transform	825:833	arg1	infrared					835:842	infrared	835:842	transform infrared spectroscopy and X-ray diffraction methods	825:885	HAnp-CMC scaffolds were characterized using high-resolution scanning electron microscopy combined with energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction methods.
34617273	6	79	theme	chain	1281:1285	arg1	reaction					1287:1294	real-time quantitative polymerase chain reaction	1247:1294	real-time quantitative polymerase chain reaction	1247:1294	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	17	80	theme	dentine	2847:2853	arg1	regeneration					2855:2866	dentine regeneration	2847:2866	dentine regeneration	2847:2866	The 1:5 HAnp-CMC composite has the potential to serve as a promising scaffold for dentine regeneration.
34617273	10	81	theme	porous	1816:1821	arg1	microstructure					1823:1836	a highly interconnected porous microstructure	1792:1836	a highly interconnected porous microstructure	1792:1836	The ultrastructure of all the scaffolds revealed a highly interconnected porous microstructure, whilst the chemical characterization displayed specific functional groups of both HAnp and CMC.
34617273	1	82	theme	composite	215:223	arg1	scaffolds					225:233	porous biomineralizable composite scaffolds	191:233	porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC)	191:321	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	9	83	theme	1.67	1737:1740	arg1	ratio					1728:1732	calcium/phosphorus (Ca/P) molar ratio	1696:1732	calcium/phosphorus (Ca/P) molar ratio	1696:1732	RESULTS Synthesized hydroxyapatite (HAp) comprised crystals ranging from 20 to 50 nm (HAnp) with spherulite morphology and calcium/phosphorus (Ca/P) molar ratio of 1.67.
34617273	9	83	theme	1.67	1737:1740	arg1	morphology					1681:1690	spherulite morphology	1670:1690	spherulite morphology	1670:1690	RESULTS Synthesized hydroxyapatite (HAp) comprised crystals ranging from 20 to 50 nm (HAnp) with spherulite morphology and calcium/phosphorus (Ca/P) molar ratio of 1.67.
34617273	6	84	theme	quantitative	1257:1268	arg1	reaction					1287:1294	real-time quantitative polymerase chain reaction	1247:1294	real-time quantitative polymerase chain reaction	1247:1294	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	9	85	with	50 nm	1652:1656	arg1	ratio					1728:1732	calcium/phosphorus (Ca/P) molar ratio	1696:1732	calcium/phosphorus (Ca/P) molar ratio	1696:1732	RESULTS Synthesized hydroxyapatite (HAp) comprised crystals ranging from 20 to 50 nm (HAnp) with spherulite morphology and calcium/phosphorus (Ca/P) molar ratio of 1.67.
34617273	9	85	with	50 nm	1652:1656	arg1	morphology					1681:1690	spherulite morphology	1670:1690	spherulite morphology	1670:1690	RESULTS Synthesized hydroxyapatite (HAp) comprised crystals ranging from 20 to 50 nm (HAnp) with spherulite morphology and calcium/phosphorus (Ca/P) molar ratio of 1.67.
34617273	15	86	dep	higher	2585:2590	arg1	p < .01					2593:2599	p < .01	2593:2599	p < .01	2593:2599	Mean fluorescence intensity of the VEGF expression at day 21 (32.5) was also significantly higher (p < .01) than at day 7 (12.54).
34617273	1	87	theme	derived	255:261	arg1	nano-hydroxyapatite					263:281	eggshell derived nano-hydroxyapatite	246:281	eggshell derived nano-hydroxyapatite (HAnp)	246:288	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	1	87	theme	derived	255:261	arg1	HAnp					284:287	HAnp	284:287	HAnp	284:287	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	7	88	theme	hoc	1502:1504	arg1	test					1506:1509	appropriate post hoc test	1485:1509	appropriate post hoc test (Dunn)	1485:1516	For intergroup and intragroup comparisons, Kruskal-Wallis and Friedman tests were employed, respectively, followed by appropriate post hoc test (Dunn).
34617273	7	88	theme	hoc	1502:1504	arg1	Dunn					1512:1515	Dunn	1512:1515	Dunn	1512:1515	For intergroup and intragroup comparisons, Kruskal-Wallis and Friedman tests were employed, respectively, followed by appropriate post hoc test (Dunn).
34617273	2	89	theme	METHODOLOGY	374:384	arg1	Nano-hydroxyapatite					386:404	METHODOLOGY Nano-hydroxyapatite	374:404	METHODOLOGY Nano-hydroxyapatite	374:404	METHODOLOGY Nano-hydroxyapatite was derived from eggshells using a simple combustion method and CMC was prepared from chitosan through a chemical route.
34617273	7	90	theme	appropriate	1485:1495	arg1	test					1506:1509	appropriate post hoc test	1485:1509	appropriate post hoc test (Dunn)	1485:1516	For intergroup and intragroup comparisons, Kruskal-Wallis and Friedman tests were employed, respectively, followed by appropriate post hoc test (Dunn).
34617273	7	90	theme	appropriate	1485:1495	arg1	Dunn					1512:1515	Dunn	1512:1515	Dunn	1512:1515	For intergroup and intragroup comparisons, Kruskal-Wallis and Friedman tests were employed, respectively, followed by appropriate post hoc test (Dunn).
34617273	13	91	theme	viability/proliferation	2222:2244	arg1	rates					2246:2250	Cell viability/proliferation rates	2217:2250	Cell viability/proliferation rates of hDPSCs loaded 1:5 HAnp-CMC at 21st day	2217:2292	Cell viability/proliferation rates of hDPSCs loaded 1:5 HAnp-CMC at 21st day was significantly greater than that at 7th day (p < .05).
34617273	10	92	theme	specific	1886:1893	arg1	CMC					1930:1932	CMC	1930:1932	CMC	1930:1932	The ultrastructure of all the scaffolds revealed a highly interconnected porous microstructure, whilst the chemical characterization displayed specific functional groups of both HAnp and CMC.
34617273	10	92	theme	specific	1886:1893	arg1	groups					1906:1911	specific functional groups	1886:1911	specific functional groups of both HAnp and CMC	1886:1932	The ultrastructure of all the scaffolds revealed a highly interconnected porous microstructure, whilst the chemical characterization displayed specific functional groups of both HAnp and CMC.
34617273	10	92	theme	specific	1886:1893	arg1	HAnp					1921:1924	HAnp	1921:1924	HAnp	1921:1924	The ultrastructure of all the scaffolds revealed a highly interconnected porous microstructure, whilst the chemical characterization displayed specific functional groups of both HAnp and CMC.
34617273	16	93	theme	CONCLUSION	2625:2634	arg1	hDPSCs					2636:2641	CONCLUSION hDPSCs	2625:2641	CONCLUSION hDPSCs on 1:5 HAnp-CMC scaffolds	2625:2667	CONCLUSION hDPSCs on 1:5 HAnp-CMC scaffolds displayed increased cell viability/proliferation and enhanced DSPP as well as VEGF expressions.
34617273	4	94	theme	high-resolution	720:734	arg1	Fourier					817:823	Fourier	817:823	Fourier transform infrared spectroscopy and X-ray diffraction methods	817:885	HAnp-CMC scaffolds were characterized using high-resolution scanning electron microscopy combined with energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction methods.
34617273	4	94	theme	high-resolution	720:734	arg1	microscopy					754:763	high-resolution scanning electron microscopy	720:763	high-resolution scanning electron microscopy combined with energy-dispersive X-ray spectroscopy	720:814	HAnp-CMC scaffolds were characterized using high-resolution scanning electron microscopy combined with energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction methods.
34617273	1	95	theme	odontogenic	150:160	arg1	abilities					178:186	odontogenic differentiation abilities	150:186	odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs)	150:371	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	4	96	theme	electron	745:752	arg1	Fourier					817:823	Fourier	817:823	Fourier transform infrared spectroscopy and X-ray diffraction methods	817:885	HAnp-CMC scaffolds were characterized using high-resolution scanning electron microscopy combined with energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction methods.
34617273	4	96	theme	electron	745:752	arg1	microscopy					754:763	high-resolution scanning electron microscopy	720:763	high-resolution scanning electron microscopy combined with energy-dispersive X-ray spectroscopy	720:814	HAnp-CMC scaffolds were characterized using high-resolution scanning electron microscopy combined with energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction methods.
34617273	15	97	theme	Mean	2494:2497	arg1	intensity					2512:2520	Mean fluorescence intensity	2494:2520	Mean fluorescence intensity of the VEGF expression at day 21 (32.5)	2494:2560	Mean fluorescence intensity of the VEGF expression at day 21 (32.5) was also significantly higher (p < .01) than at day 7 (12.54).
34617273	5	98	theme	simulated	953:961	arg1	fluid					968:972	simulated body fluid	953:972	simulated body fluid for 21 days	953:984	In vitro bioactivity was determined following the interaction in simulated body fluid for 21 days.
34617273	6	99	dep	viability/proliferation	1054:1076	arg1	expressions					1160:1170	expressions	1160:1170	expressions	1160:1170	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	14	100	theme	expression	2393:2402	arg1	quantification					2370:2383	The mean relative quantification	2352:2383	The mean relative quantification of DSPP expression by the scaffold	2352:2418	The mean relative quantification of DSPP expression by the scaffold was significantly higher (p < .05) on day 21 (3.16) than on day 7 (1.67).
34617273	14	100	theme	expression	2393:2402	arg1	higher					2438:2443	higher	2438:2443	higher	2438:2443	The mean relative quantification of DSPP expression by the scaffold was significantly higher (p < .05) on day 21 (3.16) than on day 7 (1.67).
34617273	1	101	theme	porous	191:196	arg1	scaffolds					225:233	porous biomineralizable composite scaffolds	191:233	porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC)	191:321	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	6	102	theme	dentine	1079:1085	arg1	DSPP					1108:1111	DSPP	1108:1111	DSPP	1108:1111	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	6	102	theme	dentine	1079:1085	arg1	sialophosphoprotein					1087:1105	dentine sialophosphoprotein	1079:1105	dentine sialophosphoprotein (DSPP)	1079:1112	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	14	103	dep	higher	2438:2443	arg1	1.67					2487:2490	1.67	2487:2490	1.67	2487:2490	The mean relative quantification of DSPP expression by the scaffold was significantly higher (p < .05) on day 21 (3.16) than on day 7 (1.67).
34617273	14	103	dep	higher	2438:2443	arg1	p < .05					2446:2452	p < .05	2446:2452	p < .05	2446:2452	The mean relative quantification of DSPP expression by the scaffold was significantly higher (p < .05) on day 21 (3.16) than on day 7 (1.67).
34617273	6	104	theme	bromide	1232:1238	arg1	assay					1240:1244	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay	1178:1244	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay	1178:1244	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	17	105	theme	promising	2824:2832	arg1	scaffold					2834:2841	a promising scaffold	2822:2841	a promising scaffold for dentine regeneration	2822:2866	The 1:5 HAnp-CMC composite has the potential to serve as a promising scaffold for dentine regeneration.
34617273	14	106	theme	relative	2361:2368	arg1	quantification					2370:2383	The mean relative quantification	2352:2383	The mean relative quantification of DSPP expression by the scaffold	2352:2418	The mean relative quantification of DSPP expression by the scaffold was significantly higher (p < .05) on day 21 (3.16) than on day 7 (1.67).
34617273	14	106	theme	relative	2361:2368	arg1	higher					2438:2443	higher	2438:2443	higher	2438:2443	The mean relative quantification of DSPP expression by the scaffold was significantly higher (p < .05) on day 21 (3.16) than on day 7 (1.67).
34617273	16	107	theme	1:5	2646:2648	arg1	scaffolds					2659:2667	1:5 HAnp-CMC scaffolds	2646:2667	1:5 HAnp-CMC scaffolds	2646:2667	CONCLUSION hDPSCs on 1:5 HAnp-CMC scaffolds displayed increased cell viability/proliferation and enhanced DSPP as well as VEGF expressions.
34617273	16	108	from	hDPSCs	2636:2641	arg1	scaffolds					2659:2667	1:5 HAnp-CMC scaffolds	2646:2667	1:5 HAnp-CMC scaffolds	2646:2667	CONCLUSION hDPSCs on 1:5 HAnp-CMC scaffolds displayed increased cell viability/proliferation and enhanced DSPP as well as VEGF expressions.
34617273	11	109	dep	In	1935:1936	arg1	vitro					1938:1942	vitro	1938:1942	vitro	1938:1942	In vitro bioactivity assessment confirmed the biomineralization potential of all scaffolds with an apatite-like crystal formation on the surface.
34617273	7	110	theme	Friedman	1429:1436	arg1	tests					1438:1442	Kruskal-Wallis and Friedman tests	1410:1442	Kruskal-Wallis and Friedman tests	1410:1442	For intergroup and intragroup comparisons, Kruskal-Wallis and Friedman tests were employed, respectively, followed by appropriate post hoc test (Dunn).
34617273	2	111	theme	simple	441:446	arg1	method					459:464	a simple combustion method	439:464	a simple combustion method	439:464	METHODOLOGY Nano-hydroxyapatite was derived from eggshells using a simple combustion method and CMC was prepared from chitosan through a chemical route.
34617273	4	112	dep	Fourier	817:823	arg1	transform					825:833	transform	825:833	transform infrared spectroscopy and X-ray diffraction methods	825:885	HAnp-CMC scaffolds were characterized using high-resolution scanning electron microscopy combined with energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction methods.
34617273	7	113	theme	Kruskal-Wallis	1410:1423	arg1	tests					1438:1442	Kruskal-Wallis and Friedman tests	1410:1442	Kruskal-Wallis and Friedman tests	1410:1442	For intergroup and intragroup comparisons, Kruskal-Wallis and Friedman tests were employed, respectively, followed by appropriate post hoc test (Dunn).
34617273	0	114	theme	incorporated	37:48	arg1	scaffold					73:80	nano-hydroxyapatite incorporated carboxymethyl chitosan scaffold	17:80	nano-hydroxyapatite incorporated carboxymethyl chitosan scaffold for dentine regeneration	17:105	Eggshell derived nano-hydroxyapatite incorporated carboxymethyl chitosan scaffold for dentine regeneration: A laboratory investigation.
34617273	4	115	theme	X-ray	861:865	arg1	methods					879:885	X-ray diffraction methods	861:885	X-ray diffraction methods	861:885	HAnp-CMC scaffolds were characterized using high-resolution scanning electron microscopy combined with energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction methods.
34617273	15	116	theme	expression	2534:2543	arg1	intensity					2512:2520	Mean fluorescence intensity	2494:2520	Mean fluorescence intensity of the VEGF expression at day 21 (32.5)	2494:2560	Mean fluorescence intensity of the VEGF expression at day 21 (32.5) was also significantly higher (p < .01) than at day 7 (12.54).
34617273	6	117	theme	growth	1139:1144	arg1	VEGF					1154:1157	VEGF	1154:1157	VEGF	1154:1157	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	6	117	theme	growth	1139:1144	arg1	factor					1146:1151	vascular endothelial growth factor	1118:1151	vascular endothelial growth factor (VEGF)	1118:1158	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	17	118	theme	1:5	2769:2771	arg1	composite					2782:2790	The 1:5 HAnp-CMC composite	2765:2790	The 1:5 HAnp-CMC composite	2765:2790	The 1:5 HAnp-CMC composite has the potential to serve as a promising scaffold for dentine regeneration.
34617273	3	119	theme	stirring	640:647	arg1	methods					667:673	magnetic stirring and freeze-drying methods	631:673	magnetic stirring and freeze-drying methods	631:673	Several compositions of HAnp-CMC (0:5, 5:0, 1:5, 2:5, 3:5, 4:5 and 1:1 w/w%) scaffolds were prepared by magnetic stirring and freeze-drying methods.
34617273	4	120	theme	energy-dispersive	779:795	arg1	spectroscopy					803:814	energy-dispersive X-ray spectroscopy	779:814	energy-dispersive X-ray spectroscopy	779:814	HAnp-CMC scaffolds were characterized using high-resolution scanning electron microscopy combined with energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction methods.
34617273	8	121	theme	Significant	1519:1529	arg1	levels					1531:1536	Significant levels	1519:1536	Significant levels	1519:1536	Significant levels were set at *p < .05 and *p < .01.
34617273	0	122	theme	chitosan	64:71	arg1	scaffold					73:80	nano-hydroxyapatite incorporated carboxymethyl chitosan scaffold	17:80	nano-hydroxyapatite incorporated carboxymethyl chitosan scaffold for dentine regeneration	17:105	Eggshell derived nano-hydroxyapatite incorporated carboxymethyl chitosan scaffold for dentine regeneration: A laboratory investigation.
34617273	1	123	theme	pulp	348:351	arg1	cells					358:362	cultured human dental pulp stem cells	326:362	cultured human dental pulp stem cells (hDPSCs)	326:371	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	1	123	theme	pulp	348:351	arg1	hDPSCs					365:370	hDPSCs	365:370	hDPSCs	365:370	AIM To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
34617273	6	124	theme	vascular	1118:1125	arg1	VEGF					1154:1157	VEGF	1154:1157	VEGF	1154:1157	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	6	124	theme	vascular	1118:1125	arg1	factor					1146:1151	vascular endothelial growth factor	1118:1151	vascular endothelial growth factor (VEGF)	1118:1158	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	4	125	theme	diffraction	867:877	arg1	methods					879:885	X-ray diffraction methods	861:885	X-ray diffraction methods	861:885	HAnp-CMC scaffolds were characterized using high-resolution scanning electron microscopy combined with energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction methods.
34617273	6	126	theme	tetrazolium	1220:1230	arg1	bromide					1232:1238	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide	1178:1238	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay	1178:1244	The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days.
34617273	3	127	theme	HAnp-CMC	551:558	arg1	scaffolds					604:612	HAnp-CMC (0:5, 5:0, 1:5, 2:5, 3:5, 4:5 and 1:1 w/w%) scaffolds	551:612	HAnp-CMC (0:5, 5:0, 1:5, 2:5, 3:5, 4:5 and 1:1 w/w%) scaffolds	551:612	Several compositions of HAnp-CMC (0:5, 5:0, 1:5, 2:5, 3:5, 4:5 and 1:1 w/w%) scaffolds were prepared by magnetic stirring and freeze-drying methods.
34617273	16	128	theme	HAnp-CMC	2650:2657	arg1	scaffolds					2659:2667	1:5 HAnp-CMC scaffolds	2646:2667	1:5 HAnp-CMC scaffolds	2646:2667	CONCLUSION hDPSCs on 1:5 HAnp-CMC scaffolds displayed increased cell viability/proliferation and enhanced DSPP as well as VEGF expressions.
34617273	3	129	theme	freeze-drying	653:665	arg1	methods					667:673	magnetic stirring and freeze-drying methods	631:673	magnetic stirring and freeze-drying methods	631:673	Several compositions of HAnp-CMC (0:5, 5:0, 1:5, 2:5, 3:5, 4:5 and 1:1 w/w%) scaffolds were prepared by magnetic stirring and freeze-drying methods.
34617273	9	130	theme	calcium/phosphorus	1696:1713	arg1	ratio					1728:1732	calcium/phosphorus (Ca/P) molar ratio	1696:1732	calcium/phosphorus (Ca/P) molar ratio	1696:1732	RESULTS Synthesized hydroxyapatite (HAp) comprised crystals ranging from 20 to 50 nm (HAnp) with spherulite morphology and calcium/phosphorus (Ca/P) molar ratio of 1.67.
34617273	13	131	theme	21st	2285:2288	arg1	day					2290:2292	21st day	2285:2292	21st day	2285:2292	Cell viability/proliferation rates of hDPSCs loaded 1:5 HAnp-CMC at 21st day was significantly greater than that at 7th day (p < .05).
31972997	1	0	theme	heterostructured	159:174	arg1	Ag-NPs					234:239	Ag-NPs	234:239	Ag-NPs	234:239	A new method for the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry is reported.
31972997	1	0	theme	heterostructured	159:174	arg1	nanoparticles					219:231	stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles	152:231	stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry	152:265	A new method for the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry is reported.
31972997	1	1	theme	green	251:255	arg1	chemistry					257:265	green chemistry	251:265	green chemistry	251:265	A new method for the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry is reported.
31972997	0	2	theme	Sensing	94:100	arg1	Ability					102:108	Their Hydrogen Peroxide Sensing Ability	70:108	Their Hydrogen Peroxide Sensing Ability	70:108	Alginate-Mediated Synthesis of Hetero-Shaped Silver Nanoparticles and Their Hydrogen Peroxide Sensing Ability.
31972997	2	3	dep	Fourier	458:464	arg1	transform					466:474	transform	466:474	transform infrared spectroscopy (FTIR)	466:503	The as-prepared nanoparticles were characterized using the ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS) techniques.
31972997	2	4	theme	absorption	368:377	arg1	spectroscopy					379:390	the ultraviolet-visible (UV-Vis) absorption spectroscopy	335:390	the ultraviolet-visible (UV-Vis) absorption spectroscopy	335:390	The as-prepared nanoparticles were characterized using the ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS) techniques.
31972997	3	5	dep	range	661:665	arg1	nm					683:684	± 18-60 ± 25 nm	670:684	the range 30 ± 18-60 ± 25 nm	657:684	The results showed that the as-prepared Ag-NPs have a heterostructured morphology with particle size in the range 30 ± 18-60 ± 25 nm, showing a zeta potential of -62 mV.
31972997	2	6	theme	scattering	524:533	arg1	techniques					541:550	dynamic light scattering (DLS) techniques	510:550	dynamic light scattering (DLS) techniques	510:550	The as-prepared nanoparticles were characterized using the ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS) techniques.
31972997	7	7	theme	sensing	1091:1097	arg1	capability					1099:1108	excellent sensing capability	1081:1108	excellent sensing capability	1081:1108	The Ag-NPs also showed excellent sensing capability, with a linear response to hydrogen peroxide spanning a wide range of concentrations from 10-1 to 10-7 M, which indicates their high potential for water treatment applications, such as pollution detection and nanofiltration composites.
31972997	5	8	theme	XRD	929:931	arg1	measurements					933:944	TEM and XRD measurements	921:944	TEM and XRD measurements	921:944	The particle size and crystallinity of the as-synthesized nanoparticles were analyzed using TEM and XRD measurements, respectively.
31972997	2	9	theme	light	518:522	arg1	DLS					536:538	DLS	536:538	DLS	536:538	The as-prepared nanoparticles were characterized using the ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS) techniques.
31972997	2	9	theme	light	518:522	arg1	scattering					524:533	dynamic light scattering	510:533	dynamic light scattering (DLS) techniques	510:550	The as-prepared nanoparticles were characterized using the ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS) techniques.
31972997	2	10	theme	as-prepared	284:294	arg1	nanoparticles					296:308	The as-prepared nanoparticles	280:308	The as-prepared nanoparticles	280:308	The as-prepared nanoparticles were characterized using the ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS) techniques.
31972997	6	11	theme	FTIR	961:964	arg1	spectra					966:972	FTIR spectra	961:972	FTIR spectra	961:972	FTIR spectra confirmed the presence of alginate as capping agent to stabilize the nanoparticles.
31972997	2	12	theme	dynamic	510:516	arg1	DLS					536:538	DLS	536:538	DLS	536:538	The as-prepared nanoparticles were characterized using the ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS) techniques.
31972997	2	12	theme	dynamic	510:516	arg1	scattering					524:533	dynamic light scattering	510:533	dynamic light scattering (DLS) techniques	510:550	The as-prepared nanoparticles were characterized using the ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS) techniques.
31972997	3	13	theme	zeta	697:700	arg1	potential					702:710	a zeta potential	695:710	a zeta potential of -62 mV	695:720	The results showed that the as-prepared Ag-NPs have a heterostructured morphology with particle size in the range 30 ± 18-60 ± 25 nm, showing a zeta potential of -62 mV.
31972997	2	14	theme	electron	431:438	arg1	TEM					452:454	TEM	452:454	TEM	452:454	The as-prepared nanoparticles were characterized using the ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS) techniques.
31972997	2	14	theme	electron	431:438	arg1	microscopy					440:449	transmission electron microscopy	418:449	transmission electron microscopy (TEM)	418:455	The as-prepared nanoparticles were characterized using the ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS) techniques.
31972997	4	15	theme	nanoparticle	734:745	arg1	formation					747:755	The silver nanoparticle formation	723:755	The silver nanoparticle formation	723:755	The silver nanoparticle formation was confirmed from UV-Vis spectra showing 424 nm as maximum absorption.
31972997	5	16	theme	particle	833:840	arg1	size					842:845	particle size	833:845	particle size	833:845	The particle size and crystallinity of the as-synthesized nanoparticles were analyzed using TEM and XRD measurements, respectively.
31972997	7	17	theme	pollution	1295:1303	arg1	detection					1305:1313	pollution detection and nanofiltration composites	1295:1343	detection	1305:1313	The Ag-NPs also showed excellent sensing capability, with a linear response to hydrogen peroxide spanning a wide range of concentrations from 10-1 to 10-7 M, which indicates their high potential for water treatment applications, such as pollution detection and nanofiltration composites.
31972997	7	18	theme	excellent	1081:1089	arg1	capability					1099:1108	excellent sensing capability	1081:1108	excellent sensing capability	1081:1108	The Ag-NPs also showed excellent sensing capability, with a linear response to hydrogen peroxide spanning a wide range of concentrations from 10-1 to 10-7 M, which indicates their high potential for water treatment applications, such as pollution detection and nanofiltration composites.
31972997	4	19	theme	silver	727:732	arg1	formation					747:755	The silver nanoparticle formation	723:755	The silver nanoparticle formation	723:755	The silver nanoparticle formation was confirmed from UV-Vis spectra showing 424 nm as maximum absorption.
31972997	1	20	theme	sodium	188:193	arg1	Ag-NPs					234:239	Ag-NPs	234:239	Ag-NPs	234:239	A new method for the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry is reported.
31972997	1	20	theme	sodium	188:193	arg1	nanoparticles					219:231	stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles	152:231	stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry	152:265	A new method for the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry is reported.
31972997	3	21	with	morphology	624:633	arg1	size					649:652	particle size	640:652	particle size	640:652	The results showed that the as-prepared Ag-NPs have a heterostructured morphology with particle size in the range 30 ± 18-60 ± 25 nm, showing a zeta potential of -62 mV.
31972997	7	22	theme	water	1257:1261	arg1	applications					1273:1284	water treatment applications	1257:1284	water treatment applications	1257:1284	The Ag-NPs also showed excellent sensing capability, with a linear response to hydrogen peroxide spanning a wide range of concentrations from 10-1 to 10-7 M, which indicates their high potential for water treatment applications, such as pollution detection and nanofiltration composites.
31972997	7	22	theme	water	1257:1261	arg1	composites					1334:1343	pollution detection and nanofiltration composites	1295:1343	composites	1334:1343	The Ag-NPs also showed excellent sensing capability, with a linear response to hydrogen peroxide spanning a wide range of concentrations from 10-1 to 10-7 M, which indicates their high potential for water treatment applications, such as pollution detection and nanofiltration composites.
31972997	7	22	theme	water	1257:1261	arg1	detection					1305:1313	pollution detection and nanofiltration composites	1295:1343	detection	1305:1313	The Ag-NPs also showed excellent sensing capability, with a linear response to hydrogen peroxide spanning a wide range of concentrations from 10-1 to 10-7 M, which indicates their high potential for water treatment applications, such as pollution detection and nanofiltration composites.
31972997	7	23	theme	high	1238:1241	arg1	potential					1243:1251	their high potential	1232:1251	their high potential for water treatment applications, such as pollution detection and nanofiltration composites	1232:1343	The Ag-NPs also showed excellent sensing capability, with a linear response to hydrogen peroxide spanning a wide range of concentrations from 10-1 to 10-7 M, which indicates their high potential for water treatment applications, such as pollution detection and nanofiltration composites.
31972997	1	24	theme	alginate	195:202	arg1	Ag-NPs					234:239	Ag-NPs	234:239	Ag-NPs	234:239	A new method for the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry is reported.
31972997	1	24	theme	alginate	195:202	arg1	nanoparticles					219:231	stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles	152:231	stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry	152:265	A new method for the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry is reported.
31972997	0	25	theme	Alginate-Mediated	0:16	arg1	Synthesis					18:26	Alginate-Mediated Synthesis	0:26	Alginate-Mediated Synthesis of Hetero-Shaped Silver Nanoparticles	0:64	Alginate-Mediated Synthesis of Hetero-Shaped Silver Nanoparticles and Their Hydrogen Peroxide Sensing Ability.
31972997	4	26	theme	maximum	809:815	arg1	absorption					817:826	maximum absorption	809:826	maximum absorption	809:826	The silver nanoparticle formation was confirmed from UV-Vis spectra showing 424 nm as maximum absorption.
31972997	5	27	theme	TEM	921:923	arg1	measurements					933:944	TEM and XRD measurements	921:944	TEM and XRD measurements	921:944	The particle size and crystallinity of the as-synthesized nanoparticles were analyzed using TEM and XRD measurements, respectively.
31972997	0	28	theme	Hetero-Shaped	31:43	arg1	Nanoparticles					52:64	Hetero-Shaped Silver Nanoparticles	31:64	Hetero-Shaped Silver Nanoparticles	31:64	Alginate-Mediated Synthesis of Hetero-Shaped Silver Nanoparticles and Their Hydrogen Peroxide Sensing Ability.
31972997	3	29	theme	mV	719:720	arg1	potential					702:710	a zeta potential	695:710	a zeta potential of -62 mV	695:720	The results showed that the as-prepared Ag-NPs have a heterostructured morphology with particle size in the range 30 ± 18-60 ± 25 nm, showing a zeta potential of -62 mV.
31972997	1	30	theme	new	113:115	arg1	method					117:122	A new method	111:122	A new method for the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry	111:265	A new method for the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry is reported.
31972997	1	31	theme	-capped	204:210	arg1	Ag-NPs					234:239	Ag-NPs	234:239	Ag-NPs	234:239	A new method for the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry is reported.
31972997	1	31	theme	-capped	204:210	arg1	nanoparticles					219:231	stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles	152:231	stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry	152:265	A new method for the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry is reported.
31972997	1	32	theme	biopolymer	176:185	arg1	Ag-NPs					234:239	Ag-NPs	234:239	Ag-NPs	234:239	A new method for the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry is reported.
31972997	1	32	theme	biopolymer	176:185	arg1	nanoparticles					219:231	stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles	152:231	stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry	152:265	A new method for the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry is reported.
31972997	1	33	theme	silver	212:217	arg1	Ag-NPs					234:239	Ag-NPs	234:239	Ag-NPs	234:239	A new method for the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry is reported.
31972997	1	33	theme	silver	212:217	arg1	nanoparticles					219:231	stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles	152:231	stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry	152:265	A new method for the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry is reported.
31972997	0	34	theme	Nanoparticles	52:64	arg1	Ability					102:108	Their Hydrogen Peroxide Sensing Ability	70:108	Their Hydrogen Peroxide Sensing Ability	70:108	Alginate-Mediated Synthesis of Hetero-Shaped Silver Nanoparticles and Their Hydrogen Peroxide Sensing Ability.
31972997	0	34	theme	Nanoparticles	52:64	arg1	Synthesis					18:26	Alginate-Mediated Synthesis	0:26	Alginate-Mediated Synthesis of Hetero-Shaped Silver Nanoparticles	0:64	Alginate-Mediated Synthesis of Hetero-Shaped Silver Nanoparticles and Their Hydrogen Peroxide Sensing Ability.
31972997	5	35	theme	nanoparticles	887:899	arg1	crystallinity					851:863	crystallinity	851:863	crystallinity	851:863	The particle size and crystallinity of the as-synthesized nanoparticles were analyzed using TEM and XRD measurements, respectively.
31972997	5	35	theme	nanoparticles	887:899	arg1	size					842:845	particle size	833:845	particle size	833:845	The particle size and crystallinity of the as-synthesized nanoparticles were analyzed using TEM and XRD measurements, respectively.
31972997	2	36	theme	transmission	418:429	arg1	TEM					452:454	TEM	452:454	TEM	452:454	The as-prepared nanoparticles were characterized using the ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS) techniques.
31972997	2	36	theme	transmission	418:429	arg1	microscopy					440:449	transmission electron microscopy	418:449	transmission electron microscopy (TEM)	418:455	The as-prepared nanoparticles were characterized using the ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS) techniques.
31972997	3	37	theme	as-prepared	581:591	arg1	Ag-NPs					593:598	the as-prepared Ag-NPs	577:598	the as-prepared Ag-NPs	577:598	The results showed that the as-prepared Ag-NPs have a heterostructured morphology with particle size in the range 30 ± 18-60 ± 25 nm, showing a zeta potential of -62 mV.
31972997	1	38	theme	nanoparticles	219:231	arg1	synthesis					139:147	the simple synthesis	128:147	the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry	128:265	A new method for the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry is reported.
31972997	0	39	theme	Silver	45:50	arg1	Nanoparticles					52:64	Hetero-Shaped Silver Nanoparticles	31:64	Hetero-Shaped Silver Nanoparticles	31:64	Alginate-Mediated Synthesis of Hetero-Shaped Silver Nanoparticles and Their Hydrogen Peroxide Sensing Ability.
31972997	2	40	theme	infrared	476:483	arg1	FTIR					499:502	FTIR	499:502	FTIR	499:502	The as-prepared nanoparticles were characterized using the ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS) techniques.
31972997	2	40	theme	infrared	476:483	arg1	spectroscopy					485:496	infrared spectroscopy	476:496	infrared spectroscopy (FTIR)	476:503	The as-prepared nanoparticles were characterized using the ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS) techniques.
31972997	4	41	theme	UV-Vis	776:781	arg1	spectra					783:789	UV-Vis spectra	776:789	UV-Vis spectra showing 424 nm as maximum absorption	776:826	The silver nanoparticle formation was confirmed from UV-Vis spectra showing 424 nm as maximum absorption.
31972997	6	42	theme	capping	1012:1018	arg1	agent					1020:1024	capping agent	1012:1024	capping agent to stabilize the nanoparticles	1012:1055	FTIR spectra confirmed the presence of alginate as capping agent to stabilize the nanoparticles.
31972997	3	43	theme	particle	640:647	arg1	size					649:652	particle size	640:652	particle size	640:652	The results showed that the as-prepared Ag-NPs have a heterostructured morphology with particle size in the range 30 ± 18-60 ± 25 nm, showing a zeta potential of -62 mV.
31972997	5	44	theme	as-synthesized	872:885	arg1	nanoparticles					887:899	the as-synthesized nanoparticles	868:899	the as-synthesized nanoparticles	868:899	The particle size and crystallinity of the as-synthesized nanoparticles were analyzed using TEM and XRD measurements, respectively.
31972997	7	45	theme	linear	1118:1123	arg1	response					1125:1132	a linear response	1116:1132	a linear response to hydrogen peroxide spanning a wide range of concentrations from 10-1 to 10-7 M, which indicates their high potential for water treatment applications, such as pollution detection and nanofiltration composites	1116:1343	The Ag-NPs also showed excellent sensing capability, with a linear response to hydrogen peroxide spanning a wide range of concentrations from 10-1 to 10-7 M, which indicates their high potential for water treatment applications, such as pollution detection and nanofiltration composites.
31972997	2	46	theme	UV-Vis	360:365	arg1	spectroscopy					379:390	the ultraviolet-visible (UV-Vis) absorption spectroscopy	335:390	the ultraviolet-visible (UV-Vis) absorption spectroscopy	335:390	The as-prepared nanoparticles were characterized using the ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS) techniques.
31972997	7	47	theme	hydrogen	1137:1144	arg1	peroxide					1146:1153	hydrogen peroxide	1137:1153	hydrogen peroxide spanning a wide range of concentrations from 10-1 to 10-7 M, which indicates their high potential for water treatment applications, such as pollution detection and nanofiltration composites	1137:1343	The Ag-NPs also showed excellent sensing capability, with a linear response to hydrogen peroxide spanning a wide range of concentrations from 10-1 to 10-7 M, which indicates their high potential for water treatment applications, such as pollution detection and nanofiltration composites.
31972997	3	48	theme	heterostructured	607:622	arg1	morphology					624:633	a heterostructured morphology	605:633	a heterostructured morphology with particle size	605:652	The results showed that the as-prepared Ag-NPs have a heterostructured morphology with particle size in the range 30 ± 18-60 ± 25 nm, showing a zeta potential of -62 mV.
31972997	6	49	theme	alginate	1000:1007	arg1	presence					988:995	the presence	984:995	the presence of alginate	984:1007	FTIR spectra confirmed the presence of alginate as capping agent to stabilize the nanoparticles.
31972997	1	50	theme	simple	132:137	arg1	synthesis					139:147	the simple synthesis	128:147	the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry	128:265	A new method for the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry is reported.
31972997	2	51	theme	ultraviolet-visible	339:357	arg1	spectroscopy					379:390	the ultraviolet-visible (UV-Vis) absorption spectroscopy	335:390	the ultraviolet-visible (UV-Vis) absorption spectroscopy	335:390	The as-prepared nanoparticles were characterized using the ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS) techniques.
31972997	3	52	contain	have	600:603	arg2	morphology					624:633	a heterostructured morphology	605:633	a heterostructured morphology with particle size	605:652	The results showed that the as-prepared Ag-NPs have a heterostructured morphology with particle size in the range 30 ± 18-60 ± 25 nm, showing a zeta potential of -62 mV.
31972997	3	52	contain	have	600:603	arg1	Ag-NPs					593:598	the as-prepared Ag-NPs	577:598	the as-prepared Ag-NPs	577:598	The results showed that the as-prepared Ag-NPs have a heterostructured morphology with particle size in the range 30 ± 18-60 ± 25 nm, showing a zeta potential of -62 mV.
31972997	0	53	theme	Peroxide	85:92	arg1	Ability					102:108	Their Hydrogen Peroxide Sensing Ability	70:108	Their Hydrogen Peroxide Sensing Ability	70:108	Alginate-Mediated Synthesis of Hetero-Shaped Silver Nanoparticles and Their Hydrogen Peroxide Sensing Ability.
31972997	7	54	theme	treatment	1263:1271	arg1	applications					1273:1284	water treatment applications	1257:1284	water treatment applications	1257:1284	The Ag-NPs also showed excellent sensing capability, with a linear response to hydrogen peroxide spanning a wide range of concentrations from 10-1 to 10-7 M, which indicates their high potential for water treatment applications, such as pollution detection and nanofiltration composites.
31972997	7	54	theme	treatment	1263:1271	arg1	composites					1334:1343	pollution detection and nanofiltration composites	1295:1343	composites	1334:1343	The Ag-NPs also showed excellent sensing capability, with a linear response to hydrogen peroxide spanning a wide range of concentrations from 10-1 to 10-7 M, which indicates their high potential for water treatment applications, such as pollution detection and nanofiltration composites.
31972997	7	54	theme	treatment	1263:1271	arg1	detection					1305:1313	pollution detection and nanofiltration composites	1295:1343	detection	1305:1313	The Ag-NPs also showed excellent sensing capability, with a linear response to hydrogen peroxide spanning a wide range of concentrations from 10-1 to 10-7 M, which indicates their high potential for water treatment applications, such as pollution detection and nanofiltration composites.
31972997	2	55	theme	X-ray	393:397	arg1	XRD					412:414	XRD	412:414	XRD	412:414	The as-prepared nanoparticles were characterized using the ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS) techniques.
31972997	2	55	theme	X-ray	393:397	arg1	diffraction					399:409	X-ray diffraction	393:409	X-ray diffraction (XRD)	393:415	The as-prepared nanoparticles were characterized using the ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS) techniques.
31972997	0	56	theme	Hydrogen	76:83	arg1	Ability					102:108	Their Hydrogen Peroxide Sensing Ability	70:108	Their Hydrogen Peroxide Sensing Ability	70:108	Alginate-Mediated Synthesis of Hetero-Shaped Silver Nanoparticles and Their Hydrogen Peroxide Sensing Ability.
31972997	7	57	theme	wide	1166:1169	arg1	range					1171:1175	a wide range	1164:1175	a wide range of concentrations	1164:1193	The Ag-NPs also showed excellent sensing capability, with a linear response to hydrogen peroxide spanning a wide range of concentrations from 10-1 to 10-7 M, which indicates their high potential for water treatment applications, such as pollution detection and nanofiltration composites.
31972997	7	58	theme	nanofiltration	1319:1332	arg1	composites					1334:1343	pollution detection and nanofiltration composites	1295:1343	composites	1334:1343	The Ag-NPs also showed excellent sensing capability, with a linear response to hydrogen peroxide spanning a wide range of concentrations from 10-1 to 10-7 M, which indicates their high potential for water treatment applications, such as pollution detection and nanofiltration composites.
31972997	1	59	theme	stable	152:157	arg1	Ag-NPs					234:239	Ag-NPs	234:239	Ag-NPs	234:239	A new method for the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry is reported.
31972997	1	59	theme	stable	152:157	arg1	nanoparticles					219:231	stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles	152:231	stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry	152:265	A new method for the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry is reported.
31972997	7	60	theme	concentrations	1180:1193	arg1	range					1171:1175	a wide range	1164:1175	a wide range of concentrations	1164:1193	The Ag-NPs also showed excellent sensing capability, with a linear response to hydrogen peroxide spanning a wide range of concentrations from 10-1 to 10-7 M, which indicates their high potential for water treatment applications, such as pollution detection and nanofiltration composites.
32950870	3	0	theme	differing	334:342	arg1	backbones					355:363	differing polyphenol backbones	334:363	differing polyphenol backbones	334:363	Monoglycosylated, polyglycosylated, and aglycones with differing polyphenol backbones were studied, in addition to bulk food ingredients containing a high concentration of polyphenols.
32950870	8	1	with	polyphenols	1024:1034	arg1	2,1					1042:1044	2,1	1042:1044	2,1	1042:1044	Glycosylated polyphenols were more effective than aglycones, polyphenols with (2,1) glycosidic linkages were more effective than those with (6,1) linkages, and bulk food ingredients were the most effective at delaying sucrose crystallization.
32950870	8	1	with	polyphenols	1024:1034	arg1	linkages					1058:1065	(2,1) glycosidic linkages	1041:1065	(2,1) glycosidic linkages	1041:1065	Glycosylated polyphenols were more effective than aglycones, polyphenols with (2,1) glycosidic linkages were more effective than those with (6,1) linkages, and bulk food ingredients were the most effective at delaying sucrose crystallization.
32950870	1	2	from	crystallization	78:92	arg1	products					123:130	food products	118:130	food products	118:130	The crystallization of amorphous sucrose in food products can greatly affect the quality of foods.
32950870	3	3	theme	polyphenol	344:353	arg1	backbones					355:363	differing polyphenol backbones	334:363	differing polyphenol backbones	334:363	Monoglycosylated, polyglycosylated, and aglycones with differing polyphenol backbones were studied, in addition to bulk food ingredients containing a high concentration of polyphenols.
32950870	8	4	theme	6,1	1104:1106	arg1	linkages					1109:1116	(6,1) linkages	1103:1116	(6,1) linkages	1103:1116	Glycosylated polyphenols were more effective than aglycones, polyphenols with (2,1) glycosidic linkages were more effective than those with (6,1) linkages, and bulk food ingredients were the most effective at delaying sucrose crystallization.
32950870	7	5	theme	sucrose	906:912	arg1	crystallization					914:928	sucrose crystallization	906:928	sucrose crystallization	906:928	Structure played the most significant role in efficacy of polyphenols in delaying sucrose crystallization, more than Tg or hygroscopicity.
32950870	8	6	theme	sucrose	1181:1187	arg1	crystallization					1189:1203	sucrose crystallization	1181:1203	sucrose crystallization	1181:1203	Glycosylated polyphenols were more effective than aglycones, polyphenols with (2,1) glycosidic linkages were more effective than those with (6,1) linkages, and bulk food ingredients were the most effective at delaying sucrose crystallization.
32950870	0	7	from	Effects	0:6	arg1	crystallization					26:40	crystallization	26:40	crystallization of amorphous sucrose lyophiles	26:71	Effects of polyphenols on crystallization of amorphous sucrose lyophiles.
32950870	3	8	contain	containing	416:425	arg2	concentration					434:446	a high concentration	427:446	a high concentration of polyphenols	427:461	Monoglycosylated, polyglycosylated, and aglycones with differing polyphenol backbones were studied, in addition to bulk food ingredients containing a high concentration of polyphenols.
32950870	3	8	contain	containing	416:425	arg1	ingredients					404:414	bulk food ingredients	394:414	bulk food ingredients containing a high concentration of polyphenols	394:461	Monoglycosylated, polyglycosylated, and aglycones with differing polyphenol backbones were studied, in addition to bulk food ingredients containing a high concentration of polyphenols.
32950870	6	9	dep	6.4x	794:797	arg1	to					791:792	to	791:792	to	791:792	Polyphenol addition delayed sucrose crystallization by up to 6.4x compared to the control.
32950870	4	10	theme	x-ray	604:608	arg1	diffraction					610:620	x-ray diffraction	604:620	x-ray diffraction	604:620	Solutions containing sucrose with and without polyphenols (1 and 5%) were lyophilized, stored in RH-controlled desiccators, and analyzed by x-ray diffraction.
32950870	5	11	theme	differential	678:689	arg1	calorimetry					700:710	differential scanning calorimetry	678:710	differential scanning calorimetry	678:710	Moisture sorption studies, Karl Fischer titration, and differential scanning calorimetry were also completed.
32950870	5	12	theme	Moisture	623:630	arg1	studies					641:647	Moisture sorption studies	623:647	Moisture sorption studies	623:647	Moisture sorption studies, Karl Fischer titration, and differential scanning calorimetry were also completed.
32950870	5	13	theme	sorption	632:639	arg1	studies					641:647	Moisture sorption studies	623:647	Moisture sorption studies	623:647	Moisture sorption studies, Karl Fischer titration, and differential scanning calorimetry were also completed.
32950870	6	14	theme	Polyphenol	733:742	arg1	addition					744:751	Polyphenol addition	733:751	Polyphenol addition	733:751	Polyphenol addition delayed sucrose crystallization by up to 6.4x compared to the control.
32950870	1	15	theme	foods	166:170	arg1	quality					155:161	the quality	151:161	the quality of foods	151:170	The crystallization of amorphous sucrose in food products can greatly affect the quality of foods.
32950870	2	16	from	effects	201:207	arg1	crystallization					231:245	the crystallization	227:245	the crystallization of amorphous sucrose lyophiles	227:276	This study investigated the effects of polyphenols on the crystallization of amorphous sucrose lyophiles.
32950870	3	17	theme	food	399:402	arg1	ingredients					404:414	bulk food ingredients	394:414	bulk food ingredients containing a high concentration of polyphenols	394:461	Monoglycosylated, polyglycosylated, and aglycones with differing polyphenol backbones were studied, in addition to bulk food ingredients containing a high concentration of polyphenols.
32950870	5	18	theme	Karl	650:653	arg1	titration					663:671	Karl Fischer titration	650:671	Karl Fischer titration	650:671	Moisture sorption studies, Karl Fischer titration, and differential scanning calorimetry were also completed.
32950870	7	19	theme	polyphenols	882:892	arg1	role					862:865	the most significant role	841:865	the most significant role in efficacy of polyphenols	841:892	Structure played the most significant role in efficacy of polyphenols in delaying sucrose crystallization, more than Tg or hygroscopicity.
32950870	3	20	with	aglycones	319:327	arg1	backbones					355:363	differing polyphenol backbones	334:363	differing polyphenol backbones	334:363	Monoglycosylated, polyglycosylated, and aglycones with differing polyphenol backbones were studied, in addition to bulk food ingredients containing a high concentration of polyphenols.
32950870	4	21	theme	RH-controlled	561:573	arg1	desiccators					575:585	RH-controlled desiccators	561:585	RH-controlled desiccators	561:585	Solutions containing sucrose with and without polyphenols (1 and 5%) were lyophilized, stored in RH-controlled desiccators, and analyzed by x-ray diffraction.
32950870	5	22	theme	Fischer	655:661	arg1	titration					663:671	Karl Fischer titration	650:671	Karl Fischer titration	650:671	Moisture sorption studies, Karl Fischer titration, and differential scanning calorimetry were also completed.
32950870	1	23	theme	amorphous	97:105	arg1	sucrose					107:113	amorphous sucrose	97:113	amorphous sucrose	97:113	The crystallization of amorphous sucrose in food products can greatly affect the quality of foods.
32950870	3	24	theme	polyphenols	451:461	arg1	concentration					434:446	a high concentration	427:446	a high concentration of polyphenols	427:461	Monoglycosylated, polyglycosylated, and aglycones with differing polyphenol backbones were studied, in addition to bulk food ingredients containing a high concentration of polyphenols.
32950870	8	25	theme	glycosidic	1047:1056	arg1	2,1					1042:1044	2,1	1042:1044	2,1	1042:1044	Glycosylated polyphenols were more effective than aglycones, polyphenols with (2,1) glycosidic linkages were more effective than those with (6,1) linkages, and bulk food ingredients were the most effective at delaying sucrose crystallization.
32950870	8	25	theme	glycosidic	1047:1056	arg1	linkages					1058:1065	(2,1) glycosidic linkages	1041:1065	(2,1) glycosidic linkages	1041:1065	Glycosylated polyphenols were more effective than aglycones, polyphenols with (2,1) glycosidic linkages were more effective than those with (6,1) linkages, and bulk food ingredients were the most effective at delaying sucrose crystallization.
32950870	1	26	theme	sucrose	107:113	arg1	crystallization					78:92	The crystallization	74:92	The crystallization of amorphous sucrose in food products	74:130	The crystallization of amorphous sucrose in food products can greatly affect the quality of foods.
32950870	3	27	theme	bulk	394:397	arg1	ingredients					404:414	bulk food ingredients	394:414	bulk food ingredients containing a high concentration of polyphenols	394:461	Monoglycosylated, polyglycosylated, and aglycones with differing polyphenol backbones were studied, in addition to bulk food ingredients containing a high concentration of polyphenols.
32950870	7	28	theme	significant	850:860	arg1	role					862:865	the most significant role	841:865	the most significant role in efficacy of polyphenols	841:892	Structure played the most significant role in efficacy of polyphenols in delaying sucrose crystallization, more than Tg or hygroscopicity.
32950870	3	29	theme	high	429:432	arg1	concentration					434:446	a high concentration	427:446	a high concentration of polyphenols	427:461	Monoglycosylated, polyglycosylated, and aglycones with differing polyphenol backbones were studied, in addition to bulk food ingredients containing a high concentration of polyphenols.
32950870	8	30	theme	food	1128:1131	arg1	effective					1159:1167	effective	1159:1167	effective	1159:1167	Glycosylated polyphenols were more effective than aglycones, polyphenols with (2,1) glycosidic linkages were more effective than those with (6,1) linkages, and bulk food ingredients were the most effective at delaying sucrose crystallization.
32950870	8	30	theme	food	1128:1131	arg1	ingredients					1133:1143	bulk food ingredients	1123:1143	bulk food ingredients	1123:1143	Glycosylated polyphenols were more effective than aglycones, polyphenols with (2,1) glycosidic linkages were more effective than those with (6,1) linkages, and bulk food ingredients were the most effective at delaying sucrose crystallization.
32950870	4	31	contain	containing	474:483	arg2	sucrose					485:491	sucrose	485:491	sucrose	485:491	Solutions containing sucrose with and without polyphenols (1 and 5%) were lyophilized, stored in RH-controlled desiccators, and analyzed by x-ray diffraction.
32950870	4	31	contain	containing	474:483	arg1	Solutions					464:472	Solutions	464:472	Solutions containing sucrose with and without polyphenols (1 and 5%)	464:531	Solutions containing sucrose with and without polyphenols (1 and 5%) were lyophilized, stored in RH-controlled desiccators, and analyzed by x-ray diffraction.
32950870	7	32	from	role	862:865	arg1	efficacy					870:877	efficacy	870:877	efficacy	870:877	Structure played the most significant role in efficacy of polyphenols in delaying sucrose crystallization, more than Tg or hygroscopicity.
32950870	8	33	theme	Glycosylated	963:974	arg1	polyphenols					976:986	Glycosylated polyphenols	963:986	Glycosylated polyphenols	963:986	Glycosylated polyphenols were more effective than aglycones, polyphenols with (2,1) glycosidic linkages were more effective than those with (6,1) linkages, and bulk food ingredients were the most effective at delaying sucrose crystallization.
32950870	0	34	theme	sucrose	55:61	arg1	lyophiles					63:71	amorphous sucrose lyophiles	45:71	amorphous sucrose lyophiles	45:71	Effects of polyphenols on crystallization of amorphous sucrose lyophiles.
32950870	5	35	theme	scanning	691:698	arg1	calorimetry					700:710	differential scanning calorimetry	678:710	differential scanning calorimetry	678:710	Moisture sorption studies, Karl Fischer titration, and differential scanning calorimetry were also completed.
32950870	1	36	theme	food	118:121	arg1	products					123:130	food products	118:130	food products	118:130	The crystallization of amorphous sucrose in food products can greatly affect the quality of foods.
32950870	0	37	theme	amorphous	45:53	arg1	lyophiles					63:71	amorphous sucrose lyophiles	45:71	amorphous sucrose lyophiles	45:71	Effects of polyphenols on crystallization of amorphous sucrose lyophiles.
32950870	8	38	theme	bulk	1123:1126	arg1	effective					1159:1167	effective	1159:1167	effective	1159:1167	Glycosylated polyphenols were more effective than aglycones, polyphenols with (2,1) glycosidic linkages were more effective than those with (6,1) linkages, and bulk food ingredients were the most effective at delaying sucrose crystallization.
32950870	8	38	theme	bulk	1123:1126	arg1	ingredients					1133:1143	bulk food ingredients	1123:1143	bulk food ingredients	1123:1143	Glycosylated polyphenols were more effective than aglycones, polyphenols with (2,1) glycosidic linkages were more effective than those with (6,1) linkages, and bulk food ingredients were the most effective at delaying sucrose crystallization.
32950870	2	39	theme	polyphenols	212:222	arg1	effects					201:207	the effects	197:207	the effects of polyphenols on the crystallization of amorphous sucrose lyophiles	197:276	This study investigated the effects of polyphenols on the crystallization of amorphous sucrose lyophiles.
32950870	2	40	theme	lyophiles	268:276	arg1	crystallization					231:245	the crystallization	227:245	the crystallization of amorphous sucrose lyophiles	227:276	This study investigated the effects of polyphenols on the crystallization of amorphous sucrose lyophiles.
32950870	2	41	theme	sucrose	260:266	arg1	lyophiles					268:276	amorphous sucrose lyophiles	250:276	amorphous sucrose lyophiles	250:276	This study investigated the effects of polyphenols on the crystallization of amorphous sucrose lyophiles.
32950870	2	42	theme	amorphous	250:258	arg1	lyophiles					268:276	amorphous sucrose lyophiles	250:276	amorphous sucrose lyophiles	250:276	This study investigated the effects of polyphenols on the crystallization of amorphous sucrose lyophiles.
32950870	0	43	theme	lyophiles	63:71	arg1	crystallization					26:40	crystallization	26:40	crystallization of amorphous sucrose lyophiles	26:71	Effects of polyphenols on crystallization of amorphous sucrose lyophiles.
32950870	0	44	theme	polyphenols	11:21	arg1	Effects					0:6	Effects	0:6	Effects of polyphenols on crystallization of amorphous sucrose lyophiles	0:71	Effects of polyphenols on crystallization of amorphous sucrose lyophiles.
32950870	6	45	theme	sucrose	761:767	arg1	crystallization					769:783	sucrose crystallization	761:783	sucrose crystallization	761:783	Polyphenol addition delayed sucrose crystallization by up to 6.4x compared to the control.
34096099	0	0	theme	Oral	92:95	arg1	Forms					104:108	Formulate Nanocrystalline Oral Dosage Forms	66:108	Formulate Nanocrystalline Oral Dosage Forms	66:108	Design and Use of a Thermogelling Methylcellulose Nanoemulsion to Formulate Nanocrystalline Oral Dosage Forms.
34096099	7	1	theme	fast	1506:1509	arg1	release					1511:1517	tunable fast release	1498:1517	tunable fast release	1498:1517	Overall, the proposed thermogelling nanoemulsion platform not only broadens the applications of thermoresponsive nanoemulsions but also shows great promise for more efficient formulation of oral drug products with high quality and tunable fast release.
34096099	7	2	with	formulation	1442:1452	arg1	quality					1486:1492	high quality	1481:1492	high quality	1481:1492	Overall, the proposed thermogelling nanoemulsion platform not only broadens the applications of thermoresponsive nanoemulsions but also shows great promise for more efficient formulation of oral drug products with high quality and tunable fast release.
34096099	7	2	with	formulation	1442:1452	arg1	release					1511:1517	tunable fast release	1498:1517	tunable fast release	1498:1517	Overall, the proposed thermogelling nanoemulsion platform not only broadens the applications of thermoresponsive nanoemulsions but also shows great promise for more efficient formulation of oral drug products with high quality and tunable fast release.
34096099	1	3	theme	modern	159:164	arg1	medicine					166:173	modern medicine	159:173	modern medicine	159:173	Oral drug products have become indispensable in modern medicine because of their exceptional patient compliance.
34096099	4	4	theme	thermoresponsive	628:643	arg1	properties					645:654	the dual amphiphilic and thermoresponsive properties	603:654	the dual amphiphilic and thermoresponsive properties of methylcellulose (MC)	603:678	Using the dual amphiphilic and thermoresponsive properties of methylcellulose (MC), a new thermogelling nanoemulsion and a facile thermal dripping method are developed for efficient formulation of composite particles with the MC matrix embedded with precisely controlled API nanocrystals.
34096099	0	5	theme	Nanocrystalline	76:90	arg1	Forms					104:108	Formulate Nanocrystalline Oral Dosage Forms	66:108	Formulate Nanocrystalline Oral Dosage Forms	66:108	Design and Use of a Thermogelling Methylcellulose Nanoemulsion to Formulate Nanocrystalline Oral Dosage Forms.
34096099	6	6	theme	nanoparticle	1172:1183	arg1	forms					1165:1169	dosage forms	1158:1169	dosage forms (nanoparticle suspension, drug tablet, and oral thin film)	1158:1228	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	6	6	theme	nanoparticle	1172:1183	arg1	suspension					1185:1194	nanoparticle suspension	1172:1194	nanoparticle suspension	1172:1194	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	6	7	theme	forms	1165:1169	arg1	film					1224:1227	oral thin film	1214:1227	oral thin film	1214:1227	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	6	7	theme	forms	1165:1169	arg1	variety					1147:1153	a wide variety	1140:1153	a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film)	1140:1228	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	6	7	theme	forms	1165:1169	arg1	forms					1165:1169	dosage forms	1158:1169	dosage forms (nanoparticle suspension, drug tablet, and oral thin film)	1158:1228	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	6	7	theme	forms	1165:1169	arg1	suspension					1185:1194	nanoparticle suspension	1172:1194	nanoparticle suspension	1172:1194	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	6	7	theme	forms	1165:1169	arg1	tablet					1202:1207	drug tablet	1197:1207	drug tablet	1197:1207	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	7	8	theme	great	1409:1413	arg1	promise					1415:1421	great promise	1409:1421	great promise for more efficient formulation of oral drug products with high quality and tunable fast release	1409:1517	Overall, the proposed thermogelling nanoemulsion platform not only broadens the applications of thermoresponsive nanoemulsions but also shows great promise for more efficient formulation of oral drug products with high quality and tunable fast release.
34096099	4	9	theme	MC	823:824	arg1	matrix					826:831	the MC matrix	819:831	the MC matrix	819:831	Using the dual amphiphilic and thermoresponsive properties of methylcellulose (MC), a new thermogelling nanoemulsion and a facile thermal dripping method are developed for efficient formulation of composite particles with the MC matrix embedded with precisely controlled API nanocrystals.
34096099	4	10	theme	particles	804:812	arg1	formulation					779:789	efficient formulation	769:789	efficient formulation of composite particles with the MC matrix	769:831	Using the dual amphiphilic and thermoresponsive properties of methylcellulose (MC), a new thermogelling nanoemulsion and a facile thermal dripping method are developed for efficient formulation of composite particles with the MC matrix embedded with precisely controlled API nanocrystals.
34096099	6	11	theme	processing	1062:1071	arg1	approach					1073:1080	the versatile thermal processing approach	1040:1080	the versatile thermal processing approach	1040:1080	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	0	12	theme	Dosage	97:102	arg1	Forms					104:108	Formulate Nanocrystalline Oral Dosage Forms	66:108	Formulate Nanocrystalline Oral Dosage Forms	66:108	Design and Use of a Thermogelling Methylcellulose Nanoemulsion to Formulate Nanocrystalline Oral Dosage Forms.
34096099	7	13	theme	nanoemulsion	1303:1314	arg1	platform					1316:1323	the proposed thermogelling nanoemulsion platform	1276:1323	the proposed thermogelling nanoemulsion platform	1276:1323	Overall, the proposed thermogelling nanoemulsion platform not only broadens the applications of thermoresponsive nanoemulsions but also shows great promise for more efficient formulation of oral drug products with high quality and tunable fast release.
34096099	2	14	theme	significant	382:392	arg1	challenges					394:403	significant challenges	382:403	significant challenges	382:403	However, poor bioavailability of ubiquitous low-water-soluble active pharmaceutical ingredients (APIs) and lack of efficient oral drug formulations remain as significant challenges.
34096099	4	15	theme	efficient	769:777	arg1	formulation					779:789	efficient formulation	769:789	efficient formulation of composite particles with the MC matrix	769:831	Using the dual amphiphilic and thermoresponsive properties of methylcellulose (MC), a new thermogelling nanoemulsion and a facile thermal dripping method are developed for efficient formulation of composite particles with the MC matrix embedded with precisely controlled API nanocrystals.
34096099	3	16	theme	several	541:547	arg1	steps					560:564	several processing steps	541:564	several processing steps	541:564	Nanocrystalline formulations are an attractive route to increase API solubility, but typically require abrasive mechanical milling and several processing steps to create an oral dosage form.
34096099	5	17	theme	fast	898:901	arg1	release					915:921	a fast and tunable release	896:921	a fast and tunable release performance	896:933	Moreover, a fast and tunable release performance is achieved with the combination of a fast-eroding MC matrix and fast-dissolving API nanocrystals.
34096099	4	18	theme	composite	794:802	arg1	particles					804:812	composite particles	794:812	composite particles with the MC matrix	794:831	Using the dual amphiphilic and thermoresponsive properties of methylcellulose (MC), a new thermogelling nanoemulsion and a facile thermal dripping method are developed for efficient formulation of composite particles with the MC matrix embedded with precisely controlled API nanocrystals.
34096099	3	19	theme	oral	579:582	arg1	form					591:594	an oral dosage form	576:594	an oral dosage form	576:594	Nanocrystalline formulations are an attractive route to increase API solubility, but typically require abrasive mechanical milling and several processing steps to create an oral dosage form.
34096099	4	20	theme	facile	720:725	arg1	method					744:749	a facile thermal dripping method	718:749	a facile thermal dripping method	718:749	Using the dual amphiphilic and thermoresponsive properties of methylcellulose (MC), a new thermogelling nanoemulsion and a facile thermal dripping method are developed for efficient formulation of composite particles with the MC matrix embedded with precisely controlled API nanocrystals.
34096099	5	21	theme	release	915:921	arg1	performance					923:933	a fast and tunable release performance	896:933	a fast and tunable release performance	896:933	Moreover, a fast and tunable release performance is achieved with the combination of a fast-eroding MC matrix and fast-dissolving API nanocrystals.
34096099	6	22	theme	thermal	1054:1060	arg1	approach					1073:1080	the versatile thermal processing approach	1040:1080	the versatile thermal processing approach	1040:1080	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	6	23	theme	drug	1197:1200	arg1	forms					1165:1169	dosage forms	1158:1169	dosage forms (nanoparticle suspension, drug tablet, and oral thin film)	1158:1228	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	6	23	theme	drug	1197:1200	arg1	tablet					1202:1207	drug tablet	1197:1207	drug tablet	1197:1207	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	2	24	theme	ingredients	308:318	arg1	bioavailability					238:252	poor bioavailability	233:252	poor bioavailability of ubiquitous low-water-soluble active pharmaceutical ingredients (APIs)	233:325	However, poor bioavailability of ubiquitous low-water-soluble active pharmaceutical ingredients (APIs) and lack of efficient oral drug formulations remain as significant challenges.
34096099	2	24	theme	ingredients	308:318	arg1	lack					331:334	lack	331:334	lack of efficient oral drug formulations	331:370	However, poor bioavailability of ubiquitous low-water-soluble active pharmaceutical ingredients (APIs) and lack of efficient oral drug formulations remain as significant challenges.
34096099	4	25	theme	dual	607:610	arg1	properties					645:654	the dual amphiphilic and thermoresponsive properties	603:654	the dual amphiphilic and thermoresponsive properties of methylcellulose (MC)	603:678	Using the dual amphiphilic and thermoresponsive properties of methylcellulose (MC), a new thermogelling nanoemulsion and a facile thermal dripping method are developed for efficient formulation of composite particles with the MC matrix embedded with precisely controlled API nanocrystals.
34096099	6	26	theme	versatile	1044:1052	arg1	approach					1073:1080	the versatile thermal processing approach	1040:1080	the versatile thermal processing approach	1040:1080	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	2	27	theme	efficient	339:347	arg1	formulations					359:370	efficient oral drug formulations	339:370	efficient oral drug formulations	339:370	However, poor bioavailability of ubiquitous low-water-soluble active pharmaceutical ingredients (APIs) and lack of efficient oral drug formulations remain as significant challenges.
34096099	3	28	theme	API	471:473	arg1	solubility					475:484	API solubility	471:484	API solubility	471:484	Nanocrystalline formulations are an attractive route to increase API solubility, but typically require abrasive mechanical milling and several processing steps to create an oral dosage form.
34096099	4	29	with	embedded	833:840	arg1	nanocrystals					872:883	precisely controlled API nanocrystals	847:883	precisely controlled API nanocrystals	847:883	Using the dual amphiphilic and thermoresponsive properties of methylcellulose (MC), a new thermogelling nanoemulsion and a facile thermal dripping method are developed for efficient formulation of composite particles with the MC matrix embedded with precisely controlled API nanocrystals.
34096099	5	30	theme	fast-dissolving	1000:1014	arg1	nanocrystals					1020:1031	fast-dissolving API nanocrystals	1000:1031	fast-dissolving API nanocrystals	1000:1031	Moreover, a fast and tunable release performance is achieved with the combination of a fast-eroding MC matrix and fast-dissolving API nanocrystals.
34096099	5	31	theme	MC	986:987	arg1	matrix					989:994	a fast-eroding MC matrix	971:994	a fast-eroding MC matrix	971:994	Moreover, a fast and tunable release performance is achieved with the combination of a fast-eroding MC matrix and fast-dissolving API nanocrystals.
34096099	1	32	theme	exceptional	192:202	arg1	compliance					212:221	their exceptional patient compliance	186:221	their exceptional patient compliance	186:221	Oral drug products have become indispensable in modern medicine because of their exceptional patient compliance.
34096099	3	33	theme	Nanocrystalline	406:420	arg1	route					453:457	an attractive route	439:457	an attractive route to increase API solubility	439:484	Nanocrystalline formulations are an attractive route to increase API solubility, but typically require abrasive mechanical milling and several processing steps to create an oral dosage form.
34096099	3	33	theme	Nanocrystalline	406:420	arg1	formulations					422:433	Nanocrystalline formulations	406:433	Nanocrystalline formulations	406:433	Nanocrystalline formulations are an attractive route to increase API solubility, but typically require abrasive mechanical milling and several processing steps to create an oral dosage form.
34096099	4	34	theme	API	868:870	arg1	nanocrystals					872:883	precisely controlled API nanocrystals	847:883	precisely controlled API nanocrystals	847:883	Using the dual amphiphilic and thermoresponsive properties of methylcellulose (MC), a new thermogelling nanoemulsion and a facile thermal dripping method are developed for efficient formulation of composite particles with the MC matrix embedded with precisely controlled API nanocrystals.
34096099	5	35	theme	matrix	989:994	arg1	combination					956:966	the combination	952:966	the combination of a fast-eroding MC matrix and fast-dissolving API nanocrystals	952:1031	Moreover, a fast and tunable release performance is achieved with the combination of a fast-eroding MC matrix and fast-dissolving API nanocrystals.
34096099	5	36	theme	API	1016:1018	arg1	nanocrystals					1020:1031	fast-dissolving API nanocrystals	1000:1031	fast-dissolving API nanocrystals	1000:1031	Moreover, a fast and tunable release performance is achieved with the combination of a fast-eroding MC matrix and fast-dissolving API nanocrystals.
34096099	5	37	theme	nanocrystals	1020:1031	arg1	combination					956:966	the combination	952:966	the combination of a fast-eroding MC matrix and fast-dissolving API nanocrystals	952:1031	Moreover, a fast and tunable release performance is achieved with the combination of a fast-eroding MC matrix and fast-dissolving API nanocrystals.
34096099	1	38	theme	patient	204:210	arg1	compliance					212:221	their exceptional patient compliance	186:221	their exceptional patient compliance	186:221	Oral drug products have become indispensable in modern medicine because of their exceptional patient compliance.
34096099	6	39	theme	thin	1219:1222	arg1	forms					1165:1169	dosage forms	1158:1169	dosage forms (nanoparticle suspension, drug tablet, and oral thin film)	1158:1228	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	6	39	theme	thin	1219:1222	arg1	film					1224:1227	oral thin film	1214:1227	oral thin film	1214:1227	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	4	40	theme	amphiphilic	612:622	arg1	properties					645:654	the dual amphiphilic and thermoresponsive properties	603:654	the dual amphiphilic and thermoresponsive properties of methylcellulose (MC)	603:678	Using the dual amphiphilic and thermoresponsive properties of methylcellulose (MC), a new thermogelling nanoemulsion and a facile thermal dripping method are developed for efficient formulation of composite particles with the MC matrix embedded with precisely controlled API nanocrystals.
34096099	7	41	theme	tunable	1498:1504	arg1	release					1511:1517	tunable fast release	1498:1517	tunable fast release	1498:1517	Overall, the proposed thermogelling nanoemulsion platform not only broadens the applications of thermoresponsive nanoemulsions but also shows great promise for more efficient formulation of oral drug products with high quality and tunable fast release.
34096099	1	42	theme	Oral	111:114	arg1	products					121:128	Oral drug products	111:128	Oral drug products	111:128	Oral drug products have become indispensable in modern medicine because of their exceptional patient compliance.
34096099	3	43	theme	mechanical	518:527	arg1	milling					529:535	abrasive mechanical milling	509:535	abrasive mechanical milling	509:535	Nanocrystalline formulations are an attractive route to increase API solubility, but typically require abrasive mechanical milling and several processing steps to create an oral dosage form.
34096099	0	44	theme	Thermogelling	20:32	arg1	Nanoemulsion					50:61	a Thermogelling Methylcellulose Nanoemulsion	18:61	a Thermogelling Methylcellulose Nanoemulsion	18:61	Design and Use of a Thermogelling Methylcellulose Nanoemulsion to Formulate Nanocrystalline Oral Dosage Forms.
34096099	3	45	theme	processing	549:558	arg1	steps					560:564	several processing steps	541:564	several processing steps	541:564	Nanocrystalline formulations are an attractive route to increase API solubility, but typically require abrasive mechanical milling and several processing steps to create an oral dosage form.
34096099	7	46	theme	thermoresponsive	1363:1378	arg1	nanoemulsions					1380:1392	thermoresponsive nanoemulsions	1363:1392	thermoresponsive nanoemulsions	1363:1392	Overall, the proposed thermogelling nanoemulsion platform not only broadens the applications of thermoresponsive nanoemulsions but also shows great promise for more efficient formulation of oral drug products with high quality and tunable fast release.
34096099	7	47	theme	drug	1462:1465	arg1	products					1467:1474	oral drug products	1457:1474	oral drug products	1457:1474	Overall, the proposed thermogelling nanoemulsion platform not only broadens the applications of thermoresponsive nanoemulsions but also shows great promise for more efficient formulation of oral drug products with high quality and tunable fast release.
34096099	6	48	theme	wide	1142:1145	arg1	film					1224:1227	oral thin film	1214:1227	oral thin film	1214:1227	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	6	48	theme	wide	1142:1145	arg1	variety					1147:1153	a wide variety	1140:1153	a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film)	1140:1228	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	6	48	theme	wide	1142:1145	arg1	forms					1165:1169	dosage forms	1158:1169	dosage forms (nanoparticle suspension, drug tablet, and oral thin film)	1158:1228	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	6	48	theme	wide	1142:1145	arg1	suspension					1185:1194	nanoparticle suspension	1172:1194	nanoparticle suspension	1172:1194	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	6	48	theme	wide	1142:1145	arg1	tablet					1202:1207	drug tablet	1197:1207	drug tablet	1197:1207	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	4	49	theme	thermogelling	687:699	arg1	nanoemulsion					701:712	a new thermogelling nanoemulsion	681:712	a new thermogelling nanoemulsion	681:712	Using the dual amphiphilic and thermoresponsive properties of methylcellulose (MC), a new thermogelling nanoemulsion and a facile thermal dripping method are developed for efficient formulation of composite particles with the MC matrix embedded with precisely controlled API nanocrystals.
34096099	6	50	theme	dosage	1158:1163	arg1	film					1224:1227	oral thin film	1214:1227	oral thin film	1214:1227	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	6	50	theme	dosage	1158:1163	arg1	forms					1165:1169	dosage forms	1158:1169	dosage forms (nanoparticle suspension, drug tablet, and oral thin film)	1158:1228	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	6	50	theme	dosage	1158:1163	arg1	suspension					1185:1194	nanoparticle suspension	1172:1194	nanoparticle suspension	1172:1194	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	6	50	theme	dosage	1158:1163	arg1	tablet					1202:1207	drug tablet	1197:1207	drug tablet	1197:1207	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	5	51	theme	fast-eroding	973:984	arg1	matrix					989:994	a fast-eroding MC matrix	971:994	a fast-eroding MC matrix	971:994	Moreover, a fast and tunable release performance is achieved with the combination of a fast-eroding MC matrix and fast-dissolving API nanocrystals.
34096099	4	52	theme	new	683:685	arg1	nanoemulsion					701:712	a new thermogelling nanoemulsion	681:712	a new thermogelling nanoemulsion	681:712	Using the dual amphiphilic and thermoresponsive properties of methylcellulose (MC), a new thermogelling nanoemulsion and a facile thermal dripping method are developed for efficient formulation of composite particles with the MC matrix embedded with precisely controlled API nanocrystals.
34096099	0	53	theme	Nanoemulsion	50:61	arg1	Use					11:13	Use	11:13	Use	11:13	Design and Use of a Thermogelling Methylcellulose Nanoemulsion to Formulate Nanocrystalline Oral Dosage Forms.
34096099	0	53	theme	Nanoemulsion	50:61	arg1	Design					0:5	Design	0:5	Design	0:5	Design and Use of a Thermogelling Methylcellulose Nanoemulsion to Formulate Nanocrystalline Oral Dosage Forms.
34096099	4	54	with	particles	804:812	arg1	matrix					826:831	the MC matrix	819:831	the MC matrix	819:831	Using the dual amphiphilic and thermoresponsive properties of methylcellulose (MC), a new thermogelling nanoemulsion and a facile thermal dripping method are developed for efficient formulation of composite particles with the MC matrix embedded with precisely controlled API nanocrystals.
34096099	7	55	theme	proposed	1280:1287	arg1	platform					1316:1323	the proposed thermogelling nanoemulsion platform	1276:1323	the proposed thermogelling nanoemulsion platform	1276:1323	Overall, the proposed thermogelling nanoemulsion platform not only broadens the applications of thermoresponsive nanoemulsions but also shows great promise for more efficient formulation of oral drug products with high quality and tunable fast release.
34096099	2	56	theme	poor	233:236	arg1	bioavailability					238:252	poor bioavailability	233:252	poor bioavailability of ubiquitous low-water-soluble active pharmaceutical ingredients (APIs)	233:325	However, poor bioavailability of ubiquitous low-water-soluble active pharmaceutical ingredients (APIs) and lack of efficient oral drug formulations remain as significant challenges.
34096099	1	57	theme	drug	116:119	arg1	products					121:128	Oral drug products	111:128	Oral drug products	111:128	Oral drug products have become indispensable in modern medicine because of their exceptional patient compliance.
34096099	0	58	theme	Methylcellulose	34:48	arg1	Nanoemulsion					50:61	a Thermogelling Methylcellulose Nanoemulsion	18:61	a Thermogelling Methylcellulose Nanoemulsion	18:61	Design and Use of a Thermogelling Methylcellulose Nanoemulsion to Formulate Nanocrystalline Oral Dosage Forms.
34096099	7	59	theme	oral	1457:1460	arg1	products					1467:1474	oral drug products	1457:1474	oral drug products	1457:1474	Overall, the proposed thermogelling nanoemulsion platform not only broadens the applications of thermoresponsive nanoemulsions but also shows great promise for more efficient formulation of oral drug products with high quality and tunable fast release.
34096099	2	60	theme	formulations	359:370	arg1	bioavailability					238:252	poor bioavailability	233:252	poor bioavailability of ubiquitous low-water-soluble active pharmaceutical ingredients (APIs)	233:325	However, poor bioavailability of ubiquitous low-water-soluble active pharmaceutical ingredients (APIs) and lack of efficient oral drug formulations remain as significant challenges.
34096099	2	60	theme	formulations	359:370	arg1	lack					331:334	lack	331:334	lack of efficient oral drug formulations	331:370	However, poor bioavailability of ubiquitous low-water-soluble active pharmaceutical ingredients (APIs) and lack of efficient oral drug formulations remain as significant challenges.
34096099	4	61	theme	controlled	857:866	arg1	nanocrystals					872:883	precisely controlled API nanocrystals	847:883	precisely controlled API nanocrystals	847:883	Using the dual amphiphilic and thermoresponsive properties of methylcellulose (MC), a new thermogelling nanoemulsion and a facile thermal dripping method are developed for efficient formulation of composite particles with the MC matrix embedded with precisely controlled API nanocrystals.
34096099	7	62	theme	efficient	1432:1440	arg1	formulation					1442:1452	more efficient formulation	1427:1452	more efficient formulation of oral drug products with high quality and tunable fast release	1427:1517	Overall, the proposed thermogelling nanoemulsion platform not only broadens the applications of thermoresponsive nanoemulsions but also shows great promise for more efficient formulation of oral drug products with high quality and tunable fast release.
34096099	2	63	theme	pharmaceutical	293:306	arg1	APIs					321:324	APIs	321:324	APIs	321:324	However, poor bioavailability of ubiquitous low-water-soluble active pharmaceutical ingredients (APIs) and lack of efficient oral drug formulations remain as significant challenges.
34096099	2	63	theme	pharmaceutical	293:306	arg1	ingredients					308:318	ubiquitous low-water-soluble active pharmaceutical ingredients	257:318	ubiquitous low-water-soluble active pharmaceutical ingredients (APIs)	257:325	However, poor bioavailability of ubiquitous low-water-soluble active pharmaceutical ingredients (APIs) and lack of efficient oral drug formulations remain as significant challenges.
34096099	6	64	dep	forms	1165:1169	arg1	film					1224:1227	oral thin film	1214:1227	oral thin film	1214:1227	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	6	64	dep	forms	1165:1169	arg1	forms					1165:1169	dosage forms	1158:1169	dosage forms (nanoparticle suspension, drug tablet, and oral thin film)	1158:1228	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	6	64	dep	forms	1165:1169	arg1	suspension					1185:1194	nanoparticle suspension	1172:1194	nanoparticle suspension	1172:1194	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	6	64	dep	forms	1165:1169	arg1	tablet					1202:1207	drug tablet	1197:1207	drug tablet	1197:1207	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	2	65	theme	drug	354:357	arg1	formulations					359:370	efficient oral drug formulations	339:370	efficient oral drug formulations	339:370	However, poor bioavailability of ubiquitous low-water-soluble active pharmaceutical ingredients (APIs) and lack of efficient oral drug formulations remain as significant challenges.
34096099	6	66	theme	oral	1214:1217	arg1	forms					1165:1169	dosage forms	1158:1169	dosage forms (nanoparticle suspension, drug tablet, and oral thin film)	1158:1228	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	6	66	theme	oral	1214:1217	arg1	film					1224:1227	oral thin film	1214:1227	oral thin film	1214:1227	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	7	67	theme	high	1481:1484	arg1	quality					1486:1492	high quality	1481:1492	high quality	1481:1492	Overall, the proposed thermogelling nanoemulsion platform not only broadens the applications of thermoresponsive nanoemulsions but also shows great promise for more efficient formulation of oral drug products with high quality and tunable fast release.
34096099	3	68	theme	dosage	584:589	arg1	form					591:594	an oral dosage form	576:594	an oral dosage form	576:594	Nanocrystalline formulations are an attractive route to increase API solubility, but typically require abrasive mechanical milling and several processing steps to create an oral dosage form.
34096099	2	69	theme	active	286:291	arg1	APIs					321:324	APIs	321:324	APIs	321:324	However, poor bioavailability of ubiquitous low-water-soluble active pharmaceutical ingredients (APIs) and lack of efficient oral drug formulations remain as significant challenges.
34096099	2	69	theme	active	286:291	arg1	ingredients					308:318	ubiquitous low-water-soluble active pharmaceutical ingredients	257:318	ubiquitous low-water-soluble active pharmaceutical ingredients (APIs)	257:325	However, poor bioavailability of ubiquitous low-water-soluble active pharmaceutical ingredients (APIs) and lack of efficient oral drug formulations remain as significant challenges.
34096099	2	70	theme	oral	349:352	arg1	formulations					359:370	efficient oral drug formulations	339:370	efficient oral drug formulations	339:370	However, poor bioavailability of ubiquitous low-water-soluble active pharmaceutical ingredients (APIs) and lack of efficient oral drug formulations remain as significant challenges.
34096099	4	71	theme	methylcellulose	659:673	arg1	properties					645:654	the dual amphiphilic and thermoresponsive properties	603:654	the dual amphiphilic and thermoresponsive properties of methylcellulose (MC)	603:678	Using the dual amphiphilic and thermoresponsive properties of methylcellulose (MC), a new thermogelling nanoemulsion and a facile thermal dripping method are developed for efficient formulation of composite particles with the MC matrix embedded with precisely controlled API nanocrystals.
34096099	0	72	theme	Formulate	66:74	arg1	Forms					104:108	Formulate Nanocrystalline Oral Dosage Forms	66:108	Formulate Nanocrystalline Oral Dosage Forms	66:108	Design and Use of a Thermogelling Methylcellulose Nanoemulsion to Formulate Nanocrystalline Oral Dosage Forms.
34096099	5	73	theme	tunable	907:913	arg1	release					915:921	a fast and tunable release	896:921	a fast and tunable release performance	896:933	Moreover, a fast and tunable release performance is achieved with the combination of a fast-eroding MC matrix and fast-dissolving API nanocrystals.
34096099	2	74	theme	low-water-soluble	268:284	arg1	APIs					321:324	APIs	321:324	APIs	321:324	However, poor bioavailability of ubiquitous low-water-soluble active pharmaceutical ingredients (APIs) and lack of efficient oral drug formulations remain as significant challenges.
34096099	2	74	theme	low-water-soluble	268:284	arg1	ingredients					308:318	ubiquitous low-water-soluble active pharmaceutical ingredients	257:318	ubiquitous low-water-soluble active pharmaceutical ingredients (APIs)	257:325	However, poor bioavailability of ubiquitous low-water-soluble active pharmaceutical ingredients (APIs) and lack of efficient oral drug formulations remain as significant challenges.
34096099	3	75	theme	abrasive	509:516	arg1	milling					529:535	abrasive mechanical milling	509:535	abrasive mechanical milling	509:535	Nanocrystalline formulations are an attractive route to increase API solubility, but typically require abrasive mechanical milling and several processing steps to create an oral dosage form.
34096099	4	76	theme	dripping	735:742	arg1	method					744:749	a facile thermal dripping method	718:749	a facile thermal dripping method	718:749	Using the dual amphiphilic and thermoresponsive properties of methylcellulose (MC), a new thermogelling nanoemulsion and a facile thermal dripping method are developed for efficient formulation of composite particles with the MC matrix embedded with precisely controlled API nanocrystals.
34096099	7	77	theme	nanoemulsions	1380:1392	arg1	applications					1347:1358	the applications	1343:1358	the applications of thermoresponsive nanoemulsions	1343:1392	Overall, the proposed thermogelling nanoemulsion platform not only broadens the applications of thermoresponsive nanoemulsions but also shows great promise for more efficient formulation of oral drug products with high quality and tunable fast release.
34096099	6	78	theme	thermogelling	1087:1099	arg1	nanoemulsion					1101:1112	the thermogelling nanoemulsion	1083:1112	the thermogelling nanoemulsion	1083:1112	Using the versatile thermal processing approach, the thermogelling nanoemulsion is easily formulated into a wide variety of dosage forms (nanoparticle suspension, drug tablet, and oral thin film) in a manner that avoids nanomilling.
34096099	3	79	theme	attractive	442:451	arg1	route					453:457	an attractive route	439:457	an attractive route to increase API solubility	439:484	Nanocrystalline formulations are an attractive route to increase API solubility, but typically require abrasive mechanical milling and several processing steps to create an oral dosage form.
34096099	3	79	theme	attractive	442:451	arg1	formulations					422:433	Nanocrystalline formulations	406:433	Nanocrystalline formulations	406:433	Nanocrystalline formulations are an attractive route to increase API solubility, but typically require abrasive mechanical milling and several processing steps to create an oral dosage form.
34096099	2	80	theme	ubiquitous	257:266	arg1	APIs					321:324	APIs	321:324	APIs	321:324	However, poor bioavailability of ubiquitous low-water-soluble active pharmaceutical ingredients (APIs) and lack of efficient oral drug formulations remain as significant challenges.
34096099	2	80	theme	ubiquitous	257:266	arg1	ingredients					308:318	ubiquitous low-water-soluble active pharmaceutical ingredients	257:318	ubiquitous low-water-soluble active pharmaceutical ingredients (APIs)	257:325	However, poor bioavailability of ubiquitous low-water-soluble active pharmaceutical ingredients (APIs) and lack of efficient oral drug formulations remain as significant challenges.
34096099	4	81	theme	thermal	727:733	arg1	method					744:749	a facile thermal dripping method	718:749	a facile thermal dripping method	718:749	Using the dual amphiphilic and thermoresponsive properties of methylcellulose (MC), a new thermogelling nanoemulsion and a facile thermal dripping method are developed for efficient formulation of composite particles with the MC matrix embedded with precisely controlled API nanocrystals.
34096099	7	82	theme	thermogelling	1289:1301	arg1	platform					1316:1323	the proposed thermogelling nanoemulsion platform	1276:1323	the proposed thermogelling nanoemulsion platform	1276:1323	Overall, the proposed thermogelling nanoemulsion platform not only broadens the applications of thermoresponsive nanoemulsions but also shows great promise for more efficient formulation of oral drug products with high quality and tunable fast release.
34096099	7	83	theme	products	1467:1474	arg1	formulation					1442:1452	more efficient formulation	1427:1452	more efficient formulation of oral drug products with high quality and tunable fast release	1427:1517	Overall, the proposed thermogelling nanoemulsion platform not only broadens the applications of thermoresponsive nanoemulsions but also shows great promise for more efficient formulation of oral drug products with high quality and tunable fast release.
33436213	3	0	from	DSM	470:472	arg1	LevB2286					431:438	LevB2286	431:438	LevB2286	431:438	The novel endo-levanase (LevB2286) from Azotobacter chroococcum DSM 2286, combines an exceptionally high specific activity with advantageous hydrolytic properties.
33436213	3	0	from	DSM	470:472	arg1	endo-levanase					416:428	The novel endo-levanase	406:428	The novel endo-levanase (LevB2286)	406:439	The novel endo-levanase (LevB2286) from Azotobacter chroococcum DSM 2286, combines an exceptionally high specific activity with advantageous hydrolytic properties.
33436213	1	1	theme	health-promoting	259:274	arg1	benefits					276:283	health-promoting benefits	259:283	health-promoting benefits	259:283	Prebiotics are known for their ability to modulate the composition of the human microbiome and mediate health-promoting benefits.
33436213	6	2	theme	LevB2286	845:852	arg1	activity					833:840	The combined activity	820:840	The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417	820:920	The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417 led to a one-step synthesis of levan-type FOS from sucrose.
33436213	3	3	theme	advantageous	534:545	arg1	properties					558:567	advantageous hydrolytic properties	534:567	advantageous hydrolytic properties	534:567	The novel endo-levanase (LevB2286) from Azotobacter chroococcum DSM 2286, combines an exceptionally high specific activity with advantageous hydrolytic properties.
33436213	0	4	from	Characterization	0:15	arg1	2286					75:78	Azotobacter chroococcum DSM 2286	47:78	Azotobacter chroococcum DSM 2286	47:78	Characterization of a novel endo-levanase from Azotobacter chroococcum DSM 2286 and its application for the production of prebiotic fructooligosaccharides.
33436213	7	5	theme	g	995:995	arg1	FOS					1001:1003	g L-1 FOS	995:1003	387.4 ± 17.3 g L-1 FOS	982:1003	387.4 ± 17.3 g L-1 FOS were produced within 48 h by the production strategy based on crude cell extract of recombinant Escherichia coli expressing levS1417 and levB2286 simultaneously.
33436213	4	6	attach	isolated	590:597	arg2	levan					584:588	levan	584:588	levan isolated from Timothy grass	584:616	Starting from levan isolated from Timothy grass, LevB2286 produced FOS ranging from DP 2 - 8.
33436213	4	6	attach	isolated	590:597	arg1	grass					612:616	Timothy grass	604:616	Timothy grass	604:616	Starting from levan isolated from Timothy grass, LevB2286 produced FOS ranging from DP 2 - 8.
33436213	5	7	theme	fructose	756:763	arg1	levanbiose					769:778	levanbiose	769:778	levanbiose	769:778	In contrast to endo-levanases described in the literature, LevB2286 formed minor amounts of fructose and levanbiose, even with greatly extended incubation.
33436213	5	7	theme	fructose	756:763	arg1	fructose					756:763	fructose	756:763	fructose	756:763	In contrast to endo-levanases described in the literature, LevB2286 formed minor amounts of fructose and levanbiose, even with greatly extended incubation.
33436213	5	7	theme	fructose	756:763	arg1	amounts					745:751	minor amounts	739:751	minor amounts of fructose and levanbiose	739:778	In contrast to endo-levanases described in the literature, LevB2286 formed minor amounts of fructose and levanbiose, even with greatly extended incubation.
33436213	6	8	theme	combined	824:831	arg1	activity					833:840	The combined activity	820:840	The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417	820:920	The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417 led to a one-step synthesis of levan-type FOS from sucrose.
33436213	3	9	theme	chroococcum	458:468	arg1	DSM					470:472	Azotobacter chroococcum DSM 2286	446:477	Azotobacter chroococcum DSM 2286	446:477	The novel endo-levanase (LevB2286) from Azotobacter chroococcum DSM 2286, combines an exceptionally high specific activity with advantageous hydrolytic properties.
33436213	5	10	theme	levanbiose	769:778	arg1	levanbiose					769:778	levanbiose	769:778	levanbiose	769:778	In contrast to endo-levanases described in the literature, LevB2286 formed minor amounts of fructose and levanbiose, even with greatly extended incubation.
33436213	5	10	theme	levanbiose	769:778	arg1	fructose					756:763	fructose	756:763	fructose	756:763	In contrast to endo-levanases described in the literature, LevB2286 formed minor amounts of fructose and levanbiose, even with greatly extended incubation.
33436213	5	10	theme	levanbiose	769:778	arg1	amounts					745:751	minor amounts	739:751	minor amounts of fructose and levanbiose	739:778	In contrast to endo-levanases described in the literature, LevB2286 formed minor amounts of fructose and levanbiose, even with greatly extended incubation.
33436213	7	11	theme	coli	1113:1116	arg1	extract					1078:1084	crude cell extract	1067:1084	crude cell extract of recombinant Escherichia coli expressing levS1417 and levB2286 simultaneously	1067:1164	387.4 ± 17.3 g L-1 FOS were produced within 48 h by the production strategy based on crude cell extract of recombinant Escherichia coli expressing levS1417 and levB2286 simultaneously.
33436213	3	12	theme	novel	410:414	arg1	LevB2286					431:438	LevB2286	431:438	LevB2286	431:438	The novel endo-levanase (LevB2286) from Azotobacter chroococcum DSM 2286, combines an exceptionally high specific activity with advantageous hydrolytic properties.
33436213	3	12	theme	novel	410:414	arg1	endo-levanase					416:428	The novel endo-levanase	406:428	The novel endo-levanase (LevB2286)	406:439	The novel endo-levanase (LevB2286) from Azotobacter chroococcum DSM 2286, combines an exceptionally high specific activity with advantageous hydrolytic properties.
33436213	3	13	with	activity	520:527	arg1	properties					558:567	advantageous hydrolytic properties	534:567	advantageous hydrolytic properties	534:567	The novel endo-levanase (LevB2286) from Azotobacter chroococcum DSM 2286, combines an exceptionally high specific activity with advantageous hydrolytic properties.
33436213	2	14	used	used	355:358	arg2	Endo-levanases					286:299	Endo-levanases	286:299	Endo-levanases	286:299	Endo-levanases, which hydrolyze levan into short-chain FOS, could be used for the production of levan-based prebiotics.
33436213	3	15	theme	Azotobacter	446:456	arg1	DSM					470:472	Azotobacter chroococcum DSM 2286	446:477	Azotobacter chroococcum DSM 2286	446:477	The novel endo-levanase (LevB2286) from Azotobacter chroococcum DSM 2286, combines an exceptionally high specific activity with advantageous hydrolytic properties.
33436213	0	16	theme	novel	22:26	arg1	endo-levanase					28:40	a novel endo-levanase	20:40	a novel endo-levanase from Azotobacter chroococcum DSM 2286	20:78	Characterization of a novel endo-levanase from Azotobacter chroococcum DSM 2286 and its application for the production of prebiotic fructooligosaccharides.
33436213	0	17	theme	prebiotic	122:130	arg1	fructooligosaccharides					132:153	prebiotic fructooligosaccharides	122:153	prebiotic fructooligosaccharides	122:153	Characterization of a novel endo-levanase from Azotobacter chroococcum DSM 2286 and its application for the production of prebiotic fructooligosaccharides.
33436213	2	18	theme	short-chain	329:339	arg1	FOS					341:343	short-chain FOS	329:343	short-chain FOS	329:343	Endo-levanases, which hydrolyze levan into short-chain FOS, could be used for the production of levan-based prebiotics.
33436213	7	19	theme	production	1038:1047	arg1	strategy					1049:1056	the production strategy	1034:1056	the production strategy based on crude cell extract of recombinant Escherichia coli expressing levS1417 and levB2286 simultaneously	1034:1164	387.4 ± 17.3 g L-1 FOS were produced within 48 h by the production strategy based on crude cell extract of recombinant Escherichia coli expressing levS1417 and levB2286 simultaneously.
33436213	3	20	theme	hydrolytic	547:556	arg1	properties					558:567	advantageous hydrolytic properties	534:567	advantageous hydrolytic properties	534:567	The novel endo-levanase (LevB2286) from Azotobacter chroococcum DSM 2286, combines an exceptionally high specific activity with advantageous hydrolytic properties.
33436213	5	21	theme	extended	799:806	arg1	incubation					808:817	greatly extended incubation	791:817	greatly extended incubation	791:817	In contrast to endo-levanases described in the literature, LevB2286 formed minor amounts of fructose and levanbiose, even with greatly extended incubation.
33436213	4	22	theme	Timothy	604:610	arg1	grass					612:616	Timothy grass	604:616	Timothy grass	604:616	Starting from levan isolated from Timothy grass, LevB2286 produced FOS ranging from DP 2 - 8.
33436213	5	23	theme	minor	739:743	arg1	levanbiose					769:778	levanbiose	769:778	levanbiose	769:778	In contrast to endo-levanases described in the literature, LevB2286 formed minor amounts of fructose and levanbiose, even with greatly extended incubation.
33436213	5	23	theme	minor	739:743	arg1	fructose					756:763	fructose	756:763	fructose	756:763	In contrast to endo-levanases described in the literature, LevB2286 formed minor amounts of fructose and levanbiose, even with greatly extended incubation.
33436213	5	23	theme	minor	739:743	arg1	amounts					745:751	minor amounts	739:751	minor amounts of fructose and levanbiose	739:778	In contrast to endo-levanases described in the literature, LevB2286 formed minor amounts of fructose and levanbiose, even with greatly extended incubation.
33436213	6	24	theme	japonicus	903:911	arg1	LMG					913:915	Gluconobacter japonicus LMG 1417	889:920	Gluconobacter japonicus LMG 1417	889:920	The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417 led to a one-step synthesis of levan-type FOS from sucrose.
33436213	2	25	theme	prebiotics	394:403	arg1	production					368:377	the production	364:377	the production of levan-based prebiotics	364:403	Endo-levanases, which hydrolyze levan into short-chain FOS, could be used for the production of levan-based prebiotics.
33436213	0	26	theme	endo-levanase	28:40	arg1	Characterization					0:15	Characterization	0:15	Characterization of a novel endo-levanase from Azotobacter chroococcum DSM 2286	0:78	Characterization of a novel endo-levanase from Azotobacter chroococcum DSM 2286 and its application for the production of prebiotic fructooligosaccharides.
33436213	0	26	theme	endo-levanase	28:40	arg1	application					88:98	its application	84:98	its application for the production of prebiotic fructooligosaccharides	84:153	Characterization of a novel endo-levanase from Azotobacter chroococcum DSM 2286 and its application for the production of prebiotic fructooligosaccharides.
33436213	0	27	theme	fructooligosaccharides	132:153	arg1	production					108:117	the production	104:117	the production of prebiotic fructooligosaccharides	104:153	Characterization of a novel endo-levanase from Azotobacter chroococcum DSM 2286 and its application for the production of prebiotic fructooligosaccharides.
33436213	0	28	from	2286	75:78	arg1	Characterization					0:15	Characterization	0:15	Characterization of a novel endo-levanase from Azotobacter chroococcum DSM 2286	0:78	Characterization of a novel endo-levanase from Azotobacter chroococcum DSM 2286 and its application for the production of prebiotic fructooligosaccharides.
33436213	0	28	from	2286	75:78	arg1	endo-levanase					28:40	a novel endo-levanase	20:40	a novel endo-levanase from Azotobacter chroococcum DSM 2286	20:78	Characterization of a novel endo-levanase from Azotobacter chroococcum DSM 2286 and its application for the production of prebiotic fructooligosaccharides.
33436213	0	28	from	2286	75:78	arg1	application					88:98	its application	84:98	its application for the production of prebiotic fructooligosaccharides	84:153	Characterization of a novel endo-levanase from Azotobacter chroococcum DSM 2286 and its application for the production of prebiotic fructooligosaccharides.
33436213	6	29	from	LMG	913:915	arg1	activity					833:840	The combined activity	820:840	The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417	820:920	The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417 led to a one-step synthesis of levan-type FOS from sucrose.
33436213	6	29	from	LMG	913:915	arg1	LevS1417					875:882	the levansucrase LevS1417	858:882	the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417	858:920	The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417 led to a one-step synthesis of levan-type FOS from sucrose.
33436213	6	29	from	LMG	913:915	arg1	LevB2286					845:852	LevB2286	845:852	LevB2286	845:852	The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417 led to a one-step synthesis of levan-type FOS from sucrose.
33436213	0	30	theme	chroococcum	59:69	arg1	2286					75:78	Azotobacter chroococcum DSM 2286	47:78	Azotobacter chroococcum DSM 2286	47:78	Characterization of a novel endo-levanase from Azotobacter chroococcum DSM 2286 and its application for the production of prebiotic fructooligosaccharides.
33436213	3	31	theme	specific	511:518	arg1	activity					520:527	an exceptionally high specific activity	489:527	an exceptionally high specific activity with advantageous hydrolytic properties	489:567	The novel endo-levanase (LevB2286) from Azotobacter chroococcum DSM 2286, combines an exceptionally high specific activity with advantageous hydrolytic properties.
33436213	0	32	theme	Azotobacter	47:57	arg1	2286					75:78	Azotobacter chroococcum DSM 2286	47:78	Azotobacter chroococcum DSM 2286	47:78	Characterization of a novel endo-levanase from Azotobacter chroococcum DSM 2286 and its application for the production of prebiotic fructooligosaccharides.
33436213	7	33	theme	cell	1073:1076	arg1	extract					1078:1084	crude cell extract	1067:1084	crude cell extract of recombinant Escherichia coli expressing levS1417 and levB2286 simultaneously	1067:1164	387.4 ± 17.3 g L-1 FOS were produced within 48 h by the production strategy based on crude cell extract of recombinant Escherichia coli expressing levS1417 and levB2286 simultaneously.
33436213	6	34	theme	LevS1417	875:882	arg1	activity					833:840	The combined activity	820:840	The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417	820:920	The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417 led to a one-step synthesis of levan-type FOS from sucrose.
33436213	6	35	from	activity	833:840	arg1	LMG					913:915	Gluconobacter japonicus LMG 1417	889:920	Gluconobacter japonicus LMG 1417	889:920	The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417 led to a one-step synthesis of levan-type FOS from sucrose.
33436213	6	36	theme	Gluconobacter	889:901	arg1	LMG					913:915	Gluconobacter japonicus LMG 1417	889:920	Gluconobacter japonicus LMG 1417	889:920	The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417 led to a one-step synthesis of levan-type FOS from sucrose.
33436213	6	37	theme	FOS	964:966	arg1	synthesis					940:948	a one-step synthesis	929:948	a one-step synthesis of levan-type FOS from sucrose	929:979	The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417 led to a one-step synthesis of levan-type FOS from sucrose.
33436213	6	38	theme	levansucrase	862:873	arg1	LevS1417					875:882	the levansucrase LevS1417	858:882	the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417	858:920	The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417 led to a one-step synthesis of levan-type FOS from sucrose.
33436213	7	39	dep	±	988:988	arg1	FOS					1001:1003	g L-1 FOS	995:1003	387.4 ± 17.3 g L-1 FOS	982:1003	387.4 ± 17.3 g L-1 FOS were produced within 48 h by the production strategy based on crude cell extract of recombinant Escherichia coli expressing levS1417 and levB2286 simultaneously.
33436213	1	40	theme	human	230:234	arg1	microbiome					236:245	the human microbiome	226:245	the human microbiome	226:245	Prebiotics are known for their ability to modulate the composition of the human microbiome and mediate health-promoting benefits.
33436213	6	41	from	sucrose	973:979	arg1	synthesis					940:948	a one-step synthesis	929:948	a one-step synthesis of levan-type FOS from sucrose	929:979	The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417 led to a one-step synthesis of levan-type FOS from sucrose.
33436213	6	41	from	sucrose	973:979	arg1	FOS					964:966	levan-type FOS	953:966	levan-type FOS from sucrose	953:979	The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417 led to a one-step synthesis of levan-type FOS from sucrose.
33436213	3	42	theme	high	506:509	arg1	activity					520:527	an exceptionally high specific activity	489:527	an exceptionally high specific activity with advantageous hydrolytic properties	489:567	The novel endo-levanase (LevB2286) from Azotobacter chroococcum DSM 2286, combines an exceptionally high specific activity with advantageous hydrolytic properties.
33436213	1	43	theme	microbiome	236:245	arg1	composition					211:221	the composition	207:221	the composition of the human microbiome	207:245	Prebiotics are known for their ability to modulate the composition of the human microbiome and mediate health-promoting benefits.
33436213	6	44	from	synthesis	940:948	arg1	sucrose					973:979	sucrose	973:979	sucrose	973:979	The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417 led to a one-step synthesis of levan-type FOS from sucrose.
33436213	0	45	from	application	88:98	arg1	2286					75:78	Azotobacter chroococcum DSM 2286	47:78	Azotobacter chroococcum DSM 2286	47:78	Characterization of a novel endo-levanase from Azotobacter chroococcum DSM 2286 and its application for the production of prebiotic fructooligosaccharides.
33436213	7	46	theme	crude	1067:1071	arg1	extract					1078:1084	crude cell extract	1067:1084	crude cell extract of recombinant Escherichia coli expressing levS1417 and levB2286 simultaneously	1067:1164	387.4 ± 17.3 g L-1 FOS were produced within 48 h by the production strategy based on crude cell extract of recombinant Escherichia coli expressing levS1417 and levB2286 simultaneously.
33436213	2	47	theme	levan-based	382:392	arg1	prebiotics					394:403	levan-based prebiotics	382:403	levan-based prebiotics	382:403	Endo-levanases, which hydrolyze levan into short-chain FOS, could be used for the production of levan-based prebiotics.
33436213	6	48	theme	one-step	931:938	arg1	synthesis					940:948	a one-step synthesis	929:948	a one-step synthesis of levan-type FOS from sucrose	929:979	The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417 led to a one-step synthesis of levan-type FOS from sucrose.
33436213	0	49	theme	DSM	71:73	arg1	2286					75:78	Azotobacter chroococcum DSM 2286	47:78	Azotobacter chroococcum DSM 2286	47:78	Characterization of a novel endo-levanase from Azotobacter chroococcum DSM 2286 and its application for the production of prebiotic fructooligosaccharides.
33436213	6	50	theme	levan-type	953:962	arg1	FOS					964:966	levan-type FOS	953:966	levan-type FOS from sucrose	953:979	The combined activity of LevB2286 and the levansucrase LevS1417 from Gluconobacter japonicus LMG 1417 led to a one-step synthesis of levan-type FOS from sucrose.
33436213	7	51	theme	L-1	997:999	arg1	FOS					1001:1003	g L-1 FOS	995:1003	387.4 ± 17.3 g L-1 FOS	982:1003	387.4 ± 17.3 g L-1 FOS were produced within 48 h by the production strategy based on crude cell extract of recombinant Escherichia coli expressing levS1417 and levB2286 simultaneously.
33320950	0	0	theme	zinc	68:71	arg1	composite					84:92	the toxic zinc oxide nano composite	58:92	the toxic zinc oxide nano composite towards the control of pathogenic and spoilage bacteria	58:148	Food grade zinc oxide bulk particle composite can replace the toxic zinc oxide nano composite towards the control of pathogenic and spoilage bacteria.
33320950	9	1	theme	nanomaterials	1336:1348	arg1	preparation					1350:1360	costly nanomaterials preparation	1329:1360	costly nanomaterials preparation	1329:1360	Hence, ZnO-BP-CH could be recommended as a suitable alternative to ZnO-NP-CH for future studies related to chitosan with ZnO composite to avoid costly nanomaterials preparation.
33320950	7	2	theme	composite	1011:1019	arg1	form					1021:1024	The composite form	1007:1024	The composite form of ZnO-NP-CH and ZnO-BP-CH	1007:1051	The composite form of ZnO-NP-CH and ZnO-BP-CH possessed higher antimicrobial activity than chitosan.
33320950	3	3	theme	major	493:497	arg1	limitation					499:508	a major limitation	491:508	a major limitation for commercial food applications	491:541	Moreover, the cost involved in the preparation of Zinc Oxide-Nano-Particle (ZnO-NP) is a major limitation for commercial food applications.
33320950	3	3	theme	major	493:497	arg1	cost					418:421	the cost	414:421	the cost involved in the preparation of Zinc Oxide-Nano-Particle (ZnO-NP)	414:486	Moreover, the cost involved in the preparation of Zinc Oxide-Nano-Particle (ZnO-NP) is a major limitation for commercial food applications.
33320950	9	4	with	chitosan	1292:1299	arg1	composite					1310:1318	ZnO composite	1306:1318	ZnO composite	1306:1318	Hence, ZnO-BP-CH could be recommended as a suitable alternative to ZnO-NP-CH for future studies related to chitosan with ZnO composite to avoid costly nanomaterials preparation.
33320950	6	5	theme	antimicrobial	971:983	arg1	activity					985:992	significantly higher antimicrobial activity	950:992	significantly higher antimicrobial activity	950:992	Based on the study, it was observed that in the individual form of ZnO-NP possessed significantly higher antimicrobial activity than ZnO-BP.
33320950	0	6	theme	toxic	62:66	arg1	composite					84:92	the toxic zinc oxide nano composite	58:92	the toxic zinc oxide nano composite towards the control of pathogenic and spoilage bacteria	58:148	Food grade zinc oxide bulk particle composite can replace the toxic zinc oxide nano composite towards the control of pathogenic and spoilage bacteria.
33320950	6	7	theme	higher	964:969	arg1	activity					985:992	significantly higher antimicrobial activity	950:992	significantly higher antimicrobial activity	950:992	Based on the study, it was observed that in the individual form of ZnO-NP possessed significantly higher antimicrobial activity than ZnO-BP.
33320950	0	8	theme	nano	79:82	arg1	composite					84:92	the toxic zinc oxide nano composite	58:92	the toxic zinc oxide nano composite towards the control of pathogenic and spoilage bacteria	58:148	Food grade zinc oxide bulk particle composite can replace the toxic zinc oxide nano composite towards the control of pathogenic and spoilage bacteria.
33320950	1	9	theme	better	257:262	arg1	activity					278:285	better antimicrobial activity	257:285	its better antimicrobial activity	253:285	ZnO-Nanoparticle-Chitosan (ZnO-NP-CH) composite has potential biomedical and food applications due to its better antimicrobial activity.
33320950	0	10	theme	oxide	73:77	arg1	composite					84:92	the toxic zinc oxide nano composite	58:92	the toxic zinc oxide nano composite towards the control of pathogenic and spoilage bacteria	58:148	Food grade zinc oxide bulk particle composite can replace the toxic zinc oxide nano composite towards the control of pathogenic and spoilage bacteria.
33320950	5	11	theme	food	661:664	arg1	Particles					638:646	ZnO-Bulk Particles	629:646	ZnO-Bulk Particles (ZnO-BP)	629:655	Since ZnO-Bulk Particles (ZnO-BP) are food grade and there is no study on the composite prepared from ZnO-Bulk Particle-Chitosan (ZnO-BP-CH), in the present study, antimicrobial activity was assessed for ZnO-BP-CH and compared with ZnO-NP-CH.
33320950	5	11	theme	food	661:664	arg1	grade					666:670	food grade	661:670	food grade	661:670	Since ZnO-Bulk Particles (ZnO-BP) are food grade and there is no study on the composite prepared from ZnO-Bulk Particle-Chitosan (ZnO-BP-CH), in the present study, antimicrobial activity was assessed for ZnO-BP-CH and compared with ZnO-NP-CH.
33320950	1	12	theme	antimicrobial	264:276	arg1	activity					278:285	better antimicrobial activity	257:285	its better antimicrobial activity	253:285	ZnO-Nanoparticle-Chitosan (ZnO-NP-CH) composite has potential biomedical and food applications due to its better antimicrobial activity.
33320950	8	13	theme	composite	1168:1176	arg1	forms					1178:1182	the composite forms	1164:1182	the composite forms	1164:1182	However, no significant difference was observed between the composite forms.
33320950	9	14	theme	ZnO	1306:1308	arg1	composite					1310:1318	ZnO composite	1306:1318	ZnO composite	1306:1318	Hence, ZnO-BP-CH could be recommended as a suitable alternative to ZnO-NP-CH for future studies related to chitosan with ZnO composite to avoid costly nanomaterials preparation.
33320950	3	15	theme	food	525:528	arg1	applications					530:541	commercial food applications	514:541	commercial food applications	514:541	Moreover, the cost involved in the preparation of Zinc Oxide-Nano-Particle (ZnO-NP) is a major limitation for commercial food applications.
33320950	2	16	theme	food	385:388	arg1	applications					390:401	any food applications	381:401	any food applications	381:401	However, the presence of nano-metal-oxide in the composite makes the material unsuitable for any food applications.
33320950	1	17	theme	ZnO-Nanoparticle-Chitosan	151:175	arg1	composite					189:197	ZnO-Nanoparticle-Chitosan (ZnO-NP-CH) composite	151:197	ZnO-Nanoparticle-Chitosan (ZnO-NP-CH) composite	151:197	ZnO-Nanoparticle-Chitosan (ZnO-NP-CH) composite has potential biomedical and food applications due to its better antimicrobial activity.
33320950	5	18	theme	ZnO-Bulk	725:732	arg1	ZnO-BP-CH					753:761	ZnO-BP-CH	753:761	ZnO-BP-CH	753:761	Since ZnO-Bulk Particles (ZnO-BP) are food grade and there is no study on the composite prepared from ZnO-Bulk Particle-Chitosan (ZnO-BP-CH), in the present study, antimicrobial activity was assessed for ZnO-BP-CH and compared with ZnO-NP-CH.
33320950	5	18	theme	ZnO-Bulk	725:732	arg1	Particle-Chitosan					734:750	ZnO-Bulk Particle-Chitosan	725:750	ZnO-Bulk Particle-Chitosan (ZnO-BP-CH)	725:762	Since ZnO-Bulk Particles (ZnO-BP) are food grade and there is no study on the composite prepared from ZnO-Bulk Particle-Chitosan (ZnO-BP-CH), in the present study, antimicrobial activity was assessed for ZnO-BP-CH and compared with ZnO-NP-CH.
33320950	5	19	theme	ZnO-Bulk	629:636	arg1	Particles					638:646	ZnO-Bulk Particles	629:646	ZnO-Bulk Particles (ZnO-BP)	629:655	Since ZnO-Bulk Particles (ZnO-BP) are food grade and there is no study on the composite prepared from ZnO-Bulk Particle-Chitosan (ZnO-BP-CH), in the present study, antimicrobial activity was assessed for ZnO-BP-CH and compared with ZnO-NP-CH.
33320950	5	19	theme	ZnO-Bulk	629:636	arg1	grade					666:670	food grade	661:670	food grade	661:670	Since ZnO-Bulk Particles (ZnO-BP) are food grade and there is no study on the composite prepared from ZnO-Bulk Particle-Chitosan (ZnO-BP-CH), in the present study, antimicrobial activity was assessed for ZnO-BP-CH and compared with ZnO-NP-CH.
33320950	5	19	theme	ZnO-Bulk	629:636	arg1	ZnO-BP					649:654	ZnO-BP	649:654	ZnO-BP	649:654	Since ZnO-Bulk Particles (ZnO-BP) are food grade and there is no study on the composite prepared from ZnO-Bulk Particle-Chitosan (ZnO-BP-CH), in the present study, antimicrobial activity was assessed for ZnO-BP-CH and compared with ZnO-NP-CH.
33320950	0	20	theme	grade	5:9	arg1	composite					36:44	Food grade zinc oxide bulk particle composite	0:44	Food grade zinc oxide bulk particle composite	0:44	Food grade zinc oxide bulk particle composite can replace the toxic zinc oxide nano composite towards the control of pathogenic and spoilage bacteria.
33320950	6	21	theme	individual	914:923	arg1	form					925:928	the individual form	910:928	the individual form of ZnO-NP	910:938	Based on the study, it was observed that in the individual form of ZnO-NP possessed significantly higher antimicrobial activity than ZnO-BP.
33320950	7	22	theme	higher	1063:1068	arg1	activity					1084:1091	higher antimicrobial activity	1063:1091	higher antimicrobial activity	1063:1091	The composite form of ZnO-NP-CH and ZnO-BP-CH possessed higher antimicrobial activity than chitosan.
33320950	6	23	theme	ZnO-NP	933:938	arg1	form					925:928	the individual form	910:928	the individual form of ZnO-NP	910:938	Based on the study, it was observed that in the individual form of ZnO-NP possessed significantly higher antimicrobial activity than ZnO-BP.
33320950	2	24	theme	nano-metal-oxide	313:328	arg1	presence					301:308	the presence	297:308	the presence of nano-metal-oxide in the composite	297:345	However, the presence of nano-metal-oxide in the composite makes the material unsuitable for any food applications.
33320950	1	25	theme	ZnO-NP-CH	178:186	arg1	composite					189:197	ZnO-Nanoparticle-Chitosan (ZnO-NP-CH) composite	151:197	ZnO-Nanoparticle-Chitosan (ZnO-NP-CH) composite	151:197	ZnO-Nanoparticle-Chitosan (ZnO-NP-CH) composite has potential biomedical and food applications due to its better antimicrobial activity.
33320950	0	26	theme	Food	0:3	arg1	composite					36:44	Food grade zinc oxide bulk particle composite	0:44	Food grade zinc oxide bulk particle composite	0:44	Food grade zinc oxide bulk particle composite can replace the toxic zinc oxide nano composite towards the control of pathogenic and spoilage bacteria.
33320950	4	27	theme	suitable	552:559	arg1	alternative					561:571	a suitable alternative	550:571	a suitable alternative for ZnO-NP	550:582	Hence a suitable alternative for ZnO-NP is highly needed for food application.
33320950	7	28	theme	antimicrobial	1070:1082	arg1	activity					1084:1091	higher antimicrobial activity	1063:1091	higher antimicrobial activity	1063:1091	The composite form of ZnO-NP-CH and ZnO-BP-CH possessed higher antimicrobial activity than chitosan.
33320950	0	29	theme	oxide	16:20	arg1	composite					36:44	Food grade zinc oxide bulk particle composite	0:44	Food grade zinc oxide bulk particle composite	0:44	Food grade zinc oxide bulk particle composite can replace the toxic zinc oxide nano composite towards the control of pathogenic and spoilage bacteria.
33320950	9	30	theme	costly	1329:1334	arg1	preparation					1350:1360	costly nanomaterials preparation	1329:1360	costly nanomaterials preparation	1329:1360	Hence, ZnO-BP-CH could be recommended as a suitable alternative to ZnO-NP-CH for future studies related to chitosan with ZnO composite to avoid costly nanomaterials preparation.
33320950	7	31	theme	ZnO-BP-CH	1043:1051	arg1	form					1021:1024	The composite form	1007:1024	The composite form of ZnO-NP-CH and ZnO-BP-CH	1007:1051	The composite form of ZnO-NP-CH and ZnO-BP-CH possessed higher antimicrobial activity than chitosan.
33320950	3	32	theme	Oxide-Nano-Particle	459:477	arg1	preparation					439:449	the preparation	435:449	the preparation of Zinc Oxide-Nano-Particle (ZnO-NP)	435:486	Moreover, the cost involved in the preparation of Zinc Oxide-Nano-Particle (ZnO-NP) is a major limitation for commercial food applications.
33320950	0	33	theme	zinc	11:14	arg1	composite					36:44	Food grade zinc oxide bulk particle composite	0:44	Food grade zinc oxide bulk particle composite	0:44	Food grade zinc oxide bulk particle composite can replace the toxic zinc oxide nano composite towards the control of pathogenic and spoilage bacteria.
33320950	7	34	theme	ZnO-NP-CH	1029:1037	arg1	form					1021:1024	The composite form	1007:1024	The composite form of ZnO-NP-CH and ZnO-BP-CH	1007:1051	The composite form of ZnO-NP-CH and ZnO-BP-CH possessed higher antimicrobial activity than chitosan.
33320950	7	35	contain	possessed	1053:1061	arg1	form					1021:1024	The composite form	1007:1024	The composite form of ZnO-NP-CH and ZnO-BP-CH	1007:1051	The composite form of ZnO-NP-CH and ZnO-BP-CH possessed higher antimicrobial activity than chitosan.
33320950	7	35	contain	possessed	1053:1061	arg2	activity					1084:1091	higher antimicrobial activity	1063:1091	higher antimicrobial activity	1063:1091	The composite form of ZnO-NP-CH and ZnO-BP-CH possessed higher antimicrobial activity than chitosan.
33320950	0	36	theme	particle	27:34	arg1	composite					36:44	Food grade zinc oxide bulk particle composite	0:44	Food grade zinc oxide bulk particle composite	0:44	Food grade zinc oxide bulk particle composite can replace the toxic zinc oxide nano composite towards the control of pathogenic and spoilage bacteria.
33320950	2	37	attach	presence	301:308	arg2	nano-metal-oxide					313:328	nano-metal-oxide	313:328	nano-metal-oxide	313:328	However, the presence of nano-metal-oxide in the composite makes the material unsuitable for any food applications.
33320950	2	37	attach	presence	301:308	arg1	composite					337:345	the composite	333:345	the composite	333:345	However, the presence of nano-metal-oxide in the composite makes the material unsuitable for any food applications.
33320950	0	38	theme	pathogenic	117:126	arg1	bacteria					141:148	pathogenic and spoilage bacteria	117:148	bacteria	141:148	Food grade zinc oxide bulk particle composite can replace the toxic zinc oxide nano composite towards the control of pathogenic and spoilage bacteria.
33320950	1	39	theme	potential	203:211	arg1	applications					233:244	potential biomedical and food applications	203:244	potential biomedical and food applications	203:244	ZnO-Nanoparticle-Chitosan (ZnO-NP-CH) composite has potential biomedical and food applications due to its better antimicrobial activity.
33320950	0	40	theme	bulk	22:25	arg1	composite					36:44	Food grade zinc oxide bulk particle composite	0:44	Food grade zinc oxide bulk particle composite	0:44	Food grade zinc oxide bulk particle composite can replace the toxic zinc oxide nano composite towards the control of pathogenic and spoilage bacteria.
33320950	9	41	theme	suitable	1228:1235	arg1	alternative					1237:1247	a suitable alternative	1226:1247	a suitable alternative to ZnO-NP-CH for future studies related to chitosan with ZnO composite to avoid costly nanomaterials preparation	1226:1360	Hence, ZnO-BP-CH could be recommended as a suitable alternative to ZnO-NP-CH for future studies related to chitosan with ZnO composite to avoid costly nanomaterials preparation.
33320950	9	41	theme	suitable	1228:1235	arg1	ZnO-BP-CH					1192:1200	ZnO-BP-CH	1192:1200	ZnO-BP-CH	1192:1200	Hence, ZnO-BP-CH could be recommended as a suitable alternative to ZnO-NP-CH for future studies related to chitosan with ZnO composite to avoid costly nanomaterials preparation.
33320950	5	42	theme	antimicrobial	787:799	arg1	activity					801:808	antimicrobial activity	787:808	antimicrobial activity	787:808	Since ZnO-Bulk Particles (ZnO-BP) are food grade and there is no study on the composite prepared from ZnO-Bulk Particle-Chitosan (ZnO-BP-CH), in the present study, antimicrobial activity was assessed for ZnO-BP-CH and compared with ZnO-NP-CH.
33320950	3	43	theme	Zinc	454:457	arg1	ZnO-NP					480:485	ZnO-NP	480:485	ZnO-NP	480:485	Moreover, the cost involved in the preparation of Zinc Oxide-Nano-Particle (ZnO-NP) is a major limitation for commercial food applications.
33320950	3	43	theme	Zinc	454:457	arg1	Oxide-Nano-Particle					459:477	Zinc Oxide-Nano-Particle	454:477	Zinc Oxide-Nano-Particle (ZnO-NP)	454:486	Moreover, the cost involved in the preparation of Zinc Oxide-Nano-Particle (ZnO-NP) is a major limitation for commercial food applications.
33320950	0	44	theme	spoilage	132:139	arg1	bacteria					141:148	pathogenic and spoilage bacteria	117:148	bacteria	141:148	Food grade zinc oxide bulk particle composite can replace the toxic zinc oxide nano composite towards the control of pathogenic and spoilage bacteria.
33320950	5	45	theme	present	772:778	arg1	study					780:784	the present study	768:784	the present study	768:784	Since ZnO-Bulk Particles (ZnO-BP) are food grade and there is no study on the composite prepared from ZnO-Bulk Particle-Chitosan (ZnO-BP-CH), in the present study, antimicrobial activity was assessed for ZnO-BP-CH and compared with ZnO-NP-CH.
33320950	5	46	from	study	688:692	arg1	composite					701:709	the composite	697:709	the composite prepared from ZnO-Bulk Particle-Chitosan (ZnO-BP-CH)	697:762	Since ZnO-Bulk Particles (ZnO-BP) are food grade and there is no study on the composite prepared from ZnO-Bulk Particle-Chitosan (ZnO-BP-CH), in the present study, antimicrobial activity was assessed for ZnO-BP-CH and compared with ZnO-NP-CH.
33320950	9	47	theme	related	1281:1287	arg1	studies					1273:1279	future studies	1266:1279	future studies related to chitosan with ZnO composite to avoid costly nanomaterials preparation	1266:1360	Hence, ZnO-BP-CH could be recommended as a suitable alternative to ZnO-NP-CH for future studies related to chitosan with ZnO composite to avoid costly nanomaterials preparation.
33320950	1	48	dep	potential	203:211	arg1	biomedical					213:222	biomedical	213:222	biomedical	213:222	ZnO-Nanoparticle-Chitosan (ZnO-NP-CH) composite has potential biomedical and food applications due to its better antimicrobial activity.
33320950	4	49	theme	food	605:608	arg1	application					610:620	food application	605:620	food application	605:620	Hence a suitable alternative for ZnO-NP is highly needed for food application.
33320950	0	50	theme	bacteria	141:148	arg1	control					106:112	the control	102:112	the control of pathogenic and spoilage bacteria	102:148	Food grade zinc oxide bulk particle composite can replace the toxic zinc oxide nano composite towards the control of pathogenic and spoilage bacteria.
33320950	1	51	contain	has	199:201	arg1	composite					189:197	ZnO-Nanoparticle-Chitosan (ZnO-NP-CH) composite	151:197	ZnO-Nanoparticle-Chitosan (ZnO-NP-CH) composite	151:197	ZnO-Nanoparticle-Chitosan (ZnO-NP-CH) composite has potential biomedical and food applications due to its better antimicrobial activity.
33320950	1	51	contain	has	199:201	arg2	applications					233:244	potential biomedical and food applications	203:244	potential biomedical and food applications	203:244	ZnO-Nanoparticle-Chitosan (ZnO-NP-CH) composite has potential biomedical and food applications due to its better antimicrobial activity.
33320950	3	52	theme	commercial	514:523	arg1	applications					530:541	commercial food applications	514:541	commercial food applications	514:541	Moreover, the cost involved in the preparation of Zinc Oxide-Nano-Particle (ZnO-NP) is a major limitation for commercial food applications.
33320950	1	53	theme	food	228:231	arg1	applications					233:244	potential biomedical and food applications	203:244	potential biomedical and food applications	203:244	ZnO-Nanoparticle-Chitosan (ZnO-NP-CH) composite has potential biomedical and food applications due to its better antimicrobial activity.
33320950	8	54	theme	significant	1120:1130	arg1	difference					1132:1141	no significant difference	1117:1141	no significant difference	1117:1141	However, no significant difference was observed between the composite forms.
33320950	9	55	theme	future	1266:1271	arg1	studies					1273:1279	future studies	1266:1279	future studies related to chitosan with ZnO composite to avoid costly nanomaterials preparation	1266:1360	Hence, ZnO-BP-CH could be recommended as a suitable alternative to ZnO-NP-CH for future studies related to chitosan with ZnO composite to avoid costly nanomaterials preparation.
33320950	2	56	theme	unsuitable	366:375	arg1	material					357:364	the material	353:364	the material unsuitable for any food applications	353:401	However, the presence of nano-metal-oxide in the composite makes the material unsuitable for any food applications.
33320950	2	57	from	presence	301:308	arg1	composite					337:345	the composite	333:345	the composite	333:345	However, the presence of nano-metal-oxide in the composite makes the material unsuitable for any food applications.
34202788	0	0	theme	Oxygen	102:107	arg1	Concentration					109:121	Dissolved Oxygen Concentration	92:121	Dissolved Oxygen Concentration	92:121	Fermentative Production of Fructo-Oligosaccharides Using Aureobasidium pullulans: Effect of Dissolved Oxygen Concentration and Fermentation Mode.
34202788	4	1	theme	greatest	504:511	arg1	g/L					541:543	123.2 ± 6.2 g/L	529:543	123.2 ± 6.2 g/L	529:543	The greatest FOS production (123.2 ± 6.2 g/L), with a yield of 61.6% ± 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration.
34202788	4	1	theme	greatest	504:511	arg1	production					517:526	The greatest FOS production	500:526	The greatest FOS production (123.2 ± 6.2 g/L)	500:544	The greatest FOS production (123.2 ± 6.2 g/L), with a yield of 61.6% ± 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration.
34202788	1	2	with	prebiotics	180:189	arg1	benefits					212:219	numerous health benefits	196:219	numerous health benefits	196:219	Fructo-oligosaccharides (FOS) are prebiotics with numerous health benefits.
34202788	7	3	from	%	1180:1180	arg1	product					1214:1220	the product	1210:1220	the product of repeated-batch culture	1210:1246	Additionally, the ratios of 1-fructofuranosyl nystose (GF4) and 1,1,1,1-kestohexose (GF5) were 33.8% and 23.2%, respectively, in the product of repeated-batch culture, but these compounds were not detected in batch culture.
34202788	4	4	theme	high	631:634	arg1	concentration					639:651	high DO concentration	631:651	high DO concentration	631:651	The greatest FOS production (123.2 ± 6.2 g/L), with a yield of 61.6% ± 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration.
34202788	8	5	theme	FOS	1419:1421	arg1	yield					1378:1382	the yield	1374:1382	not only the yield of FOS but also the composition of FOS	1365:1421	Thus, it can be concluded that the DO concentration affects not only the yield of FOS but also the composition of FOS with different degrees of polymerization, which is the key factor in the fermentative production of FOS with a high polymerization degree.
34202788	8	5	theme	FOS	1419:1421	arg1	composition					1404:1414	the composition	1400:1414	not only the yield of FOS but also the composition of FOS	1365:1421	Thus, it can be concluded that the DO concentration affects not only the yield of FOS but also the composition of FOS with different degrees of polymerization, which is the key factor in the fermentative production of FOS with a high polymerization degree.
34202788	0	6	theme	Dissolved	92:100	arg1	Concentration					109:121	Dissolved Oxygen Concentration	92:121	Dissolved Oxygen Concentration	92:121	Fermentative Production of Fructo-Oligosaccharides Using Aureobasidium pullulans: Effect of Dissolved Oxygen Concentration and Fermentation Mode.
34202788	5	7	theme	FOS	726:728	arg1	production					730:739	FOS production	726:739	FOS production	726:739	Furthermore, repeated-batch culture revealed that enzyme production and FOS production were not closely associated with cell growth.
34202788	8	8	with	FOS	1523:1525	arg1	degree					1554:1559	a high polymerization degree	1532:1559	a high polymerization degree	1532:1559	Thus, it can be concluded that the DO concentration affects not only the yield of FOS but also the composition of FOS with different degrees of polymerization, which is the key factor in the fermentative production of FOS with a high polymerization degree.
34202788	8	9	theme	key	1478:1480	arg1	polymerization					1449:1462	polymerization	1449:1462	polymerization	1449:1462	Thus, it can be concluded that the DO concentration affects not only the yield of FOS but also the composition of FOS with different degrees of polymerization, which is the key factor in the fermentative production of FOS with a high polymerization degree.
34202788	8	9	theme	key	1478:1480	arg1	factor					1482:1487	the key factor	1474:1487	the key factor in the fermentative production of FOS with a high polymerization degree	1474:1559	Thus, it can be concluded that the DO concentration affects not only the yield of FOS but also the composition of FOS with different degrees of polymerization, which is the key factor in the fermentative production of FOS with a high polymerization degree.
34202788	6	10	theme	repeated-batch	835:848	arg1	culture					850:856	the repeated-batch culture	831:856	the repeated-batch culture	831:856	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	6	11	theme	higher	999:1004	arg1	times					993:997	3.45% and 11.4% times	977:997	3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively	977:1078	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	4	12	theme	%	567:567	arg1	sucrose					585:591	g FOS/g sucrose	577:591	g FOS/g sucrose	577:591	The greatest FOS production (123.2 ± 6.2 g/L), with a yield of 61.6% ± 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration.
34202788	4	12	theme	%	567:567	arg1	%					574:574	61.6% ± 3.0%	563:574	61.6% ± 3.0% (g FOS/g sucrose)	563:592	The greatest FOS production (123.2 ± 6.2 g/L), with a yield of 61.6% ± 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration.
34202788	0	13	theme	Concentration	109:121	arg1	Effect					82:87	Effect	82:87	Fermentative Production of Fructo-Oligosaccharides Using Aureobasidium pullulans: Effect of Dissolved Oxygen Concentration and Fermentation Mode.	0:144	Fermentative Production of Fructo-Oligosaccharides Using Aureobasidium pullulans: Effect of Dissolved Oxygen Concentration and Fermentation Mode.
34202788	6	14	dep	concentration	805:817	arg1	DO					802:803	DO	802:803	DO	802:803	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	6	15	theme	batch	1033:1037	arg1	culture					1039:1045	the batch culture	1029:1045	the batch culture without DO control, respectively	1029:1078	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	0	16	theme	Mode	140:143	arg1	Effect					82:87	Effect	82:87	Fermentative Production of Fructo-Oligosaccharides Using Aureobasidium pullulans: Effect of Dissolved Oxygen Concentration and Fermentation Mode.	0:144	Fermentative Production of Fructo-Oligosaccharides Using Aureobasidium pullulans: Effect of Dissolved Oxygen Concentration and Fermentation Mode.
34202788	3	17	from	effects	380:386	arg1	FOS					433:435	FOS	433:435	FOS using Aureobasidium pullulans	433:465	In order to improve FOS production, the effects of DO concentration and fermentation mode on FOS using Aureobasidium pullulans were investigated in this study.
34202788	3	18	dep	concentration	394:406	arg1	DO					391:392	DO	391:392	DO	391:392	In order to improve FOS production, the effects of DO concentration and fermentation mode on FOS using Aureobasidium pullulans were investigated in this study.
34202788	6	19	theme	FOS/g	936:940	arg1	sucrose					942:948	g FOS/g sucrose	934:948	g FOS/g sucrose	934:948	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	6	19	theme	FOS/g	936:940	arg1	%					931:931	68.6% ± 2.6%	920:931	68.6% ± 2.6% (g FOS/g sucrose)	920:949	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	2	20	theme	fermentative	291:302	arg1	production					304:313	fermentative production	291:313	fermentative production of FOS	291:320	So far, the dissolved oxygen (DO) concentration control strategy for fermentative production of FOS is still unknown.
34202788	7	21	located	detected	1278:1285	arg2	compounds					1259:1267	these compounds	1253:1267	these compounds	1253:1267	Additionally, the ratios of 1-fructofuranosyl nystose (GF4) and 1,1,1,1-kestohexose (GF5) were 33.8% and 23.2%, respectively, in the product of repeated-batch culture, but these compounds were not detected in batch culture.
34202788	7	21	located	detected	1278:1285	arg1	culture					1296:1302	batch culture	1290:1302	batch culture	1290:1302	Additionally, the ratios of 1-fructofuranosyl nystose (GF4) and 1,1,1,1-kestohexose (GF5) were 33.8% and 23.2%, respectively, in the product of repeated-batch culture, but these compounds were not detected in batch culture.
34202788	1	22	theme	numerous	196:203	arg1	benefits					212:219	numerous health benefits	196:219	numerous health benefits	196:219	Fructo-oligosaccharides (FOS) are prebiotics with numerous health benefits.
34202788	0	23	theme	Fermentation	127:138	arg1	Mode					140:143	Fermentation Mode	127:143	Fermentation Mode	127:143	Fermentative Production of Fructo-Oligosaccharides Using Aureobasidium pullulans: Effect of Dissolved Oxygen Concentration and Fermentation Mode.
34202788	8	24	theme	fermentative	1496:1507	arg1	production					1509:1518	the fermentative production	1492:1518	the fermentative production of FOS with a high polymerization degree	1492:1559	Thus, it can be concluded that the DO concentration affects not only the yield of FOS but also the composition of FOS with different degrees of polymerization, which is the key factor in the fermentative production of FOS with a high polymerization degree.
34202788	0	25	theme	Aureobasidium	57:69	arg1	pullulans					71:79	Aureobasidium pullulans	57:79	Aureobasidium pullulans	57:79	Fermentative Production of Fructo-Oligosaccharides Using Aureobasidium pullulans: Effect of Dissolved Oxygen Concentration and Fermentation Mode.
34202788	7	26	from	%	1190:1190	arg1	product					1214:1220	the product	1210:1220	the product of repeated-batch culture	1210:1246	Additionally, the ratios of 1-fructofuranosyl nystose (GF4) and 1,1,1,1-kestohexose (GF5) were 33.8% and 23.2%, respectively, in the product of repeated-batch culture, but these compounds were not detected in batch culture.
34202788	1	27	theme	health	205:210	arg1	benefits					212:219	numerous health benefits	196:219	numerous health benefits	196:219	Fructo-oligosaccharides (FOS) are prebiotics with numerous health benefits.
34202788	6	28	theme	548.3	890:894	arg1	±					896:896	±	896:896	±	896:896	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	8	29	theme	FOS	1387:1389	arg1	yield					1378:1382	the yield	1374:1382	not only the yield of FOS but also the composition of FOS	1365:1421	Thus, it can be concluded that the DO concentration affects not only the yield of FOS but also the composition of FOS with different degrees of polymerization, which is the key factor in the fermentative production of FOS with a high polymerization degree.
34202788	8	29	theme	FOS	1387:1389	arg1	composition					1404:1414	the composition	1400:1414	not only the yield of FOS but also the composition of FOS	1365:1421	Thus, it can be concluded that the DO concentration affects not only the yield of FOS but also the composition of FOS with different degrees of polymerization, which is the key factor in the fermentative production of FOS with a high polymerization degree.
34202788	7	30	theme	1-fructofuranosyl	1109:1125	arg1	GF4					1136:1138	GF4	1136:1138	GF4	1136:1138	Additionally, the ratios of 1-fructofuranosyl nystose (GF4) and 1,1,1,1-kestohexose (GF5) were 33.8% and 23.2%, respectively, in the product of repeated-batch culture, but these compounds were not detected in batch culture.
34202788	7	30	theme	1-fructofuranosyl	1109:1125	arg1	nystose					1127:1133	1-fructofuranosyl nystose	1109:1133	1-fructofuranosyl nystose (GF4)	1109:1139	Additionally, the ratios of 1-fructofuranosyl nystose (GF4) and 1,1,1,1-kestohexose (GF5) were 33.8% and 23.2%, respectively, in the product of repeated-batch culture, but these compounds were not detected in batch culture.
34202788	7	31	theme	repeated-batch	1225:1238	arg1	culture					1240:1246	repeated-batch culture	1225:1246	repeated-batch culture	1225:1246	Additionally, the ratios of 1-fructofuranosyl nystose (GF4) and 1,1,1,1-kestohexose (GF5) were 33.8% and 23.2%, respectively, in the product of repeated-batch culture, but these compounds were not detected in batch culture.
34202788	0	32	theme	Fermentative	0:11	arg1	Production					13:22	Fermentative Production	0:22	Fermentative Production of Fructo-Oligosaccharides Using Aureobasidium pullulans: Effect of Dissolved Oxygen Concentration and Fermentation Mode.	0:144	Fermentative Production of Fructo-Oligosaccharides Using Aureobasidium pullulans: Effect of Dissolved Oxygen Concentration and Fermentation Mode.
34202788	6	33	theme	g/L	903:905	arg1	concentration					873:885	a maximum FOS concentration	859:885	a maximum FOS concentration of 548.3 ± 37.4 g/L	859:905	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	6	33	theme	g/L	903:905	arg1	yield					911:915	yield	911:915	yield of 68.6% ± 2.6% (g FOS/g sucrose)	911:949	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	8	34	dep	concentration	1343:1355	arg1	DO					1340:1341	DO	1340:1341	DO	1340:1341	Thus, it can be concluded that the DO concentration affects not only the yield of FOS but also the composition of FOS with different degrees of polymerization, which is the key factor in the fermentative production of FOS with a high polymerization degree.
34202788	7	35	theme	culture	1240:1246	arg1	product					1214:1220	the product	1210:1220	the product of repeated-batch culture	1210:1246	Additionally, the ratios of 1-fructofuranosyl nystose (GF4) and 1,1,1,1-kestohexose (GF5) were 33.8% and 23.2%, respectively, in the product of repeated-batch culture, but these compounds were not detected in batch culture.
34202788	0	36	theme	Fructo-Oligosaccharides	27:49	arg1	Production					13:22	Fermentative Production	0:22	Fermentative Production of Fructo-Oligosaccharides Using Aureobasidium pullulans: Effect of Dissolved Oxygen Concentration and Fermentation Mode.	0:144	Fermentative Production of Fructo-Oligosaccharides Using Aureobasidium pullulans: Effect of Dissolved Oxygen Concentration and Fermentation Mode.
34202788	2	37	theme	concentration	256:268	arg1	strategy					278:285	the dissolved oxygen (DO) concentration control strategy	230:285	the dissolved oxygen (DO) concentration control strategy for fermentative production of FOS	230:320	So far, the dissolved oxygen (DO) concentration control strategy for fermentative production of FOS is still unknown.
34202788	8	38	from	factor	1482:1487	arg1	production					1509:1518	the fermentative production	1492:1518	the fermentative production of FOS with a high polymerization degree	1492:1559	Thus, it can be concluded that the DO concentration affects not only the yield of FOS but also the composition of FOS with different degrees of polymerization, which is the key factor in the fermentative production of FOS with a high polymerization degree.
34202788	4	39	dep	concentration	639:651	arg1	DO					636:637	DO	636:637	DO	636:637	The greatest FOS production (123.2 ± 6.2 g/L), with a yield of 61.6% ± 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration.
34202788	2	40	dep	oxygen	244:249	arg1	DO					252:253	DO	252:253	DO	252:253	So far, the dissolved oxygen (DO) concentration control strategy for fermentative production of FOS is still unknown.
34202788	5	41	theme	cell	774:777	arg1	growth					779:784	cell growth	774:784	cell growth	774:784	Furthermore, repeated-batch culture revealed that enzyme production and FOS production were not closely associated with cell growth.
34202788	2	42	theme	FOS	318:320	arg1	production					304:313	fermentative production	291:313	fermentative production of FOS	291:320	So far, the dissolved oxygen (DO) concentration control strategy for fermentative production of FOS is still unknown.
34202788	4	43	theme	g	577:577	arg1	sucrose					585:591	g FOS/g sucrose	577:591	g FOS/g sucrose	577:591	The greatest FOS production (123.2 ± 6.2 g/L), with a yield of 61.6% ± 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration.
34202788	4	43	theme	g	577:577	arg1	%					574:574	61.6% ± 3.0%	563:574	61.6% ± 3.0% (g FOS/g sucrose)	563:592	The greatest FOS production (123.2 ± 6.2 g/L), with a yield of 61.6% ± 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration.
34202788	3	44	theme	fermentation	412:423	arg1	mode					425:428	fermentation mode	412:428	fermentation mode	412:428	In order to improve FOS production, the effects of DO concentration and fermentation mode on FOS using Aureobasidium pullulans were investigated in this study.
34202788	6	45	theme	%	991:991	arg1	times					993:997	3.45% and 11.4% times	977:997	3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively	977:1078	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	8	46	theme	FOS	1523:1525	arg1	production					1509:1518	the fermentative production	1492:1518	the fermentative production of FOS with a high polymerization degree	1492:1559	Thus, it can be concluded that the DO concentration affects not only the yield of FOS but also the composition of FOS with different degrees of polymerization, which is the key factor in the fermentative production of FOS with a high polymerization degree.
34202788	8	47	theme	polymerization	1449:1462	arg1	degrees					1438:1444	different degrees	1428:1444	different degrees of polymerization, which is the key factor in the fermentative production of FOS with a high polymerization degree	1428:1559	Thus, it can be concluded that the DO concentration affects not only the yield of FOS but also the composition of FOS with different degrees of polymerization, which is the key factor in the fermentative production of FOS with a high polymerization degree.
34202788	6	48	theme	g	934:934	arg1	sucrose					942:948	g FOS/g sucrose	934:948	g FOS/g sucrose	934:948	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	6	48	theme	g	934:934	arg1	%					931:931	68.6% ± 2.6%	920:931	68.6% ± 2.6% (g FOS/g sucrose)	920:949	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	3	49	theme	mode	425:428	arg1	effects					380:386	the effects	376:386	the effects of DO concentration and fermentation mode on FOS using Aureobasidium pullulans	376:465	In order to improve FOS production, the effects of DO concentration and fermentation mode on FOS using Aureobasidium pullulans were investigated in this study.
34202788	4	50	theme	±	535:535	arg1	g/L					541:543	123.2 ± 6.2 g/L	529:543	123.2 ± 6.2 g/L	529:543	The greatest FOS production (123.2 ± 6.2 g/L), with a yield of 61.6% ± 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration.
34202788	4	50	theme	±	535:535	arg1	production					517:526	The greatest FOS production	500:526	The greatest FOS production (123.2 ± 6.2 g/L)	500:544	The greatest FOS production (123.2 ± 6.2 g/L), with a yield of 61.6% ± 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration.
34202788	5	51	theme	repeated-batch	667:680	arg1	culture					682:688	repeated-batch culture	667:688	repeated-batch culture	667:688	Furthermore, repeated-batch culture revealed that enzyme production and FOS production were not closely associated with cell growth.
34202788	7	52	theme	batch	1290:1294	arg1	culture					1296:1302	batch culture	1290:1302	batch culture	1290:1302	Additionally, the ratios of 1-fructofuranosyl nystose (GF4) and 1,1,1,1-kestohexose (GF5) were 33.8% and 23.2%, respectively, in the product of repeated-batch culture, but these compounds were not detected in batch culture.
34202788	8	53	theme	high	1534:1537	arg1	degree					1554:1559	a high polymerization degree	1532:1559	a high polymerization degree	1532:1559	Thus, it can be concluded that the DO concentration affects not only the yield of FOS but also the composition of FOS with different degrees of polymerization, which is the key factor in the fermentative production of FOS with a high polymerization degree.
34202788	3	54	theme	FOS	360:362	arg1	production					364:373	FOS production	360:373	FOS production	360:373	In order to improve FOS production, the effects of DO concentration and fermentation mode on FOS using Aureobasidium pullulans were investigated in this study.
34202788	3	55	theme	concentration	394:406	arg1	effects					380:386	the effects	376:386	the effects of DO concentration and fermentation mode on FOS using Aureobasidium pullulans	376:465	In order to improve FOS production, the effects of DO concentration and fermentation mode on FOS using Aureobasidium pullulans were investigated in this study.
34202788	6	56	theme	37.4	898:901	arg1	±					896:896	±	896:896	±	896:896	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	2	57	theme	oxygen	244:249	arg1	strategy					278:285	the dissolved oxygen (DO) concentration control strategy	230:285	the dissolved oxygen (DO) concentration control strategy for fermentative production of FOS	230:320	So far, the dissolved oxygen (DO) concentration control strategy for fermentative production of FOS is still unknown.
34202788	6	58	theme	FOS	869:871	arg1	concentration					873:885	a maximum FOS concentration	859:885	a maximum FOS concentration of 548.3 ± 37.4 g/L	859:905	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	2	59	theme	dissolved	234:242	arg1	oxygen					244:249	the dissolved oxygen	230:249	the dissolved oxygen (DO) concentration control strategy for fermentative production of FOS	230:320	So far, the dissolved oxygen (DO) concentration control strategy for fermentative production of FOS is still unknown.
34202788	4	60	with	production	517:526	arg1	yield					554:558	a yield	552:558	a yield of 61.6% ± 3.0% (g FOS/g sucrose)	552:592	The greatest FOS production (123.2 ± 6.2 g/L), with a yield of 61.6% ± 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration.
34202788	6	61	theme	maximum	861:867	arg1	concentration					873:885	a maximum FOS concentration	859:885	a maximum FOS concentration of 548.3 ± 37.4 g/L	859:905	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	8	62	theme	polymerization	1539:1552	arg1	degree					1554:1559	a high polymerization degree	1532:1559	a high polymerization degree	1532:1559	Thus, it can be concluded that the DO concentration affects not only the yield of FOS but also the composition of FOS with different degrees of polymerization, which is the key factor in the fermentative production of FOS with a high polymerization degree.
34202788	7	63	theme	nystose	1127:1133	arg1	ratios					1099:1104	the ratios	1095:1104	the ratios of 1-fructofuranosyl nystose (GF4) and 1,1,1,1-kestohexose (GF5)	1095:1169	Additionally, the ratios of 1-fructofuranosyl nystose (GF4) and 1,1,1,1-kestohexose (GF5) were 33.8% and 23.2%, respectively, in the product of repeated-batch culture, but these compounds were not detected in batch culture.
34202788	7	63	theme	nystose	1127:1133	arg1	%					1180:1180	33.8%	1176:1180	33.8%	1176:1180	Additionally, the ratios of 1-fructofuranosyl nystose (GF4) and 1,1,1,1-kestohexose (GF5) were 33.8% and 23.2%, respectively, in the product of repeated-batch culture, but these compounds were not detected in batch culture.
34202788	6	64	from	concentration	805:817	arg1	culture					850:856	the repeated-batch culture	831:856	the repeated-batch culture	831:856	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	6	65	theme	%	931:931	arg1	concentration					873:885	a maximum FOS concentration	859:885	a maximum FOS concentration of 548.3 ± 37.4 g/L	859:905	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	6	65	theme	%	931:931	arg1	yield					911:915	yield	911:915	yield of 68.6% ± 2.6% (g FOS/g sucrose)	911:949	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	2	66	theme	control	270:276	arg1	strategy					278:285	the dissolved oxygen (DO) concentration control strategy	230:285	the dissolved oxygen (DO) concentration control strategy for fermentative production of FOS	230:320	So far, the dissolved oxygen (DO) concentration control strategy for fermentative production of FOS is still unknown.
34202788	6	67	dep	control	1058:1064	arg1	DO					1055:1056	DO	1055:1056	DO	1055:1056	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	4	68	theme	±	569:569	arg1	sucrose					585:591	g FOS/g sucrose	577:591	g FOS/g sucrose	577:591	The greatest FOS production (123.2 ± 6.2 g/L), with a yield of 61.6% ± 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration.
34202788	4	68	theme	±	569:569	arg1	%					574:574	61.6% ± 3.0%	563:574	61.6% ± 3.0% (g FOS/g sucrose)	563:592	The greatest FOS production (123.2 ± 6.2 g/L), with a yield of 61.6% ± 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration.
34202788	7	69	theme	1,1,1,1-kestohexose	1145:1163	arg1	ratios					1099:1104	the ratios	1095:1104	the ratios of 1-fructofuranosyl nystose (GF4) and 1,1,1,1-kestohexose (GF5)	1095:1169	Additionally, the ratios of 1-fructofuranosyl nystose (GF4) and 1,1,1,1-kestohexose (GF5) were 33.8% and 23.2%, respectively, in the product of repeated-batch culture, but these compounds were not detected in batch culture.
34202788	7	69	theme	1,1,1,1-kestohexose	1145:1163	arg1	%					1180:1180	33.8%	1176:1180	33.8%	1176:1180	Additionally, the ratios of 1-fructofuranosyl nystose (GF4) and 1,1,1,1-kestohexose (GF5) were 33.8% and 23.2%, respectively, in the product of repeated-batch culture, but these compounds were not detected in batch culture.
34202788	0	70	dep	Production	13:22	arg1	Effect					82:87	Effect	82:87	Fermentative Production of Fructo-Oligosaccharides Using Aureobasidium pullulans: Effect of Dissolved Oxygen Concentration and Fermentation Mode.	0:144	Fermentative Production of Fructo-Oligosaccharides Using Aureobasidium pullulans: Effect of Dissolved Oxygen Concentration and Fermentation Mode.
34202788	4	71	theme	FOS/g	579:583	arg1	sucrose					585:591	g FOS/g sucrose	577:591	g FOS/g sucrose	577:591	The greatest FOS production (123.2 ± 6.2 g/L), with a yield of 61.6% ± 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration.
34202788	4	71	theme	FOS/g	579:583	arg1	%					574:574	61.6% ± 3.0%	563:574	61.6% ± 3.0% (g FOS/g sucrose)	563:592	The greatest FOS production (123.2 ± 6.2 g/L), with a yield of 61.6% ± 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration.
34202788	6	72	theme	%	981:981	arg1	times					993:997	3.45% and 11.4% times	977:997	3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively	977:1078	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	3	73	theme	Aureobasidium	443:455	arg1	pullulans					457:465	Aureobasidium pullulans	443:465	Aureobasidium pullulans	443:465	In order to improve FOS production, the effects of DO concentration and fermentation mode on FOS using Aureobasidium pullulans were investigated in this study.
34202788	6	74	theme	±	926:926	arg1	sucrose					942:948	g FOS/g sucrose	934:948	g FOS/g sucrose	934:948	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	6	74	theme	±	926:926	arg1	%					931:931	68.6% ± 2.6%	920:931	68.6% ± 2.6% (g FOS/g sucrose)	920:949	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	5	75	theme	enzyme	704:709	arg1	production					711:720	enzyme production	704:720	enzyme production	704:720	Furthermore, repeated-batch culture revealed that enzyme production and FOS production were not closely associated with cell growth.
34202788	4	76	theme	FOS	513:515	arg1	g/L					541:543	123.2 ± 6.2 g/L	529:543	123.2 ± 6.2 g/L	529:543	The greatest FOS production (123.2 ± 6.2 g/L), with a yield of 61.6% ± 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration.
34202788	4	76	theme	FOS	513:515	arg1	production					517:526	The greatest FOS production	500:526	The greatest FOS production (123.2 ± 6.2 g/L)	500:544	The greatest FOS production (123.2 ± 6.2 g/L), with a yield of 61.6% ± 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration.
34202788	6	77	theme	%	924:924	arg1	sucrose					942:948	g FOS/g sucrose	934:948	g FOS/g sucrose	934:948	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	6	77	theme	%	924:924	arg1	%					931:931	68.6% ± 2.6%	920:931	68.6% ± 2.6% (g FOS/g sucrose)	920:949	By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively.
34202788	4	78	theme	batch	611:615	arg1	culture					617:623	batch culture	611:623	batch culture	611:623	The greatest FOS production (123.2 ± 6.2 g/L), with a yield of 61.6% ± 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration.
34202788	8	79	theme	different	1428:1436	arg1	degrees					1438:1444	different degrees	1428:1444	different degrees of polymerization, which is the key factor in the fermentative production of FOS with a high polymerization degree	1428:1559	Thus, it can be concluded that the DO concentration affects not only the yield of FOS but also the composition of FOS with different degrees of polymerization, which is the key factor in the fermentative production of FOS with a high polymerization degree.
34202788	4	80	theme	%	574:574	arg1	yield					554:558	a yield	552:558	a yield of 61.6% ± 3.0% (g FOS/g sucrose)	552:592	The greatest FOS production (123.2 ± 6.2 g/L), with a yield of 61.6% ± 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration.
34787399	7	0	theme	composites	1208:1217	arg1	development					1175:1185	the development	1171:1185	the development of strong wood-based composites with interesting features	1171:1243	We believe that this UV grafting strategy may facilitate the development of strong wood-based composites with interesting features.
34787399	4	1	theme	obtained	629:636	arg1	hydrogels					638:646	the obtained hydrogels	625:646	the obtained hydrogels	625:646	An important finding was that PAM-grafted cellulose nanofiber (CNF) architectures formed in the obtained hydrogels under UV irradiation, where CNFs themselves serve as both initiators and cross-linkers.
34787399	7	2	with	composites	1208:1217	arg1	features					1236:1243	interesting features	1224:1243	interesting features	1224:1243	We believe that this UV grafting strategy may facilitate the development of strong wood-based composites with interesting features.
34787399	6	3	theme	tensile	983:989	arg1	properties					991:1000	significantly higher tensile properties	962:1000	significantly higher tensile properties	962:1000	The resulting alkali-treated Wood-g-PAM hydrogels exhibited significantly higher tensile properties than those of the Wood/PAM hydrogel and were further assembled into conductive devices for sensor applications.
34787399	5	4	theme	UV	846:847	arg1	irradiation					849:859	UV irradiation	846:859	UV irradiation	846:859	Moreover, an alkali swelling treatment was utilized to improve the accessibility of the wood cell wall before UV irradiation and thus facilitate grafting efficiency.
34787399	6	5	theme	higher	976:981	arg1	properties					991:1000	significantly higher tensile properties	962:1000	significantly higher tensile properties	962:1000	The resulting alkali-treated Wood-g-PAM hydrogels exhibited significantly higher tensile properties than those of the Wood/PAM hydrogel and were further assembled into conductive devices for sensor applications.
34787399	7	6	theme	strong	1190:1195	arg1	composites					1208:1217	strong wood-based composites	1190:1217	strong wood-based composites with interesting features	1190:1243	We believe that this UV grafting strategy may facilitate the development of strong wood-based composites with interesting features.
34787399	2	7	theme	cell	274:277	arg1	wall					279:282	the wood cell wall	265:282	the wood cell wall	265:282	The hypothesis is that improved interfacial interaction between the wood cell wall and a polymer is of great importance for improving the mechanical performance.
34787399	4	8	theme	important	536:544	arg1	finding					546:552	An important finding	533:552	An important finding	533:552	An important finding was that PAM-grafted cellulose nanofiber (CNF) architectures formed in the obtained hydrogels under UV irradiation, where CNFs themselves serve as both initiators and cross-linkers.
34787399	7	9	theme	wood-based	1197:1206	arg1	composites					1208:1217	strong wood-based composites	1190:1217	strong wood-based composites with interesting features	1190:1243	We believe that this UV grafting strategy may facilitate the development of strong wood-based composites with interesting features.
34787399	4	10	theme	cellulose	575:583	arg1	CNF					596:598	CNF	596:598	CNF	596:598	An important finding was that PAM-grafted cellulose nanofiber (CNF) architectures formed in the obtained hydrogels under UV irradiation, where CNFs themselves serve as both initiators and cross-linkers.
34787399	4	10	theme	cellulose	575:583	arg1	nanofiber					585:593	PAM-grafted cellulose nanofiber	563:593	PAM-grafted cellulose nanofiber (CNF) architectures	563:613	An important finding was that PAM-grafted cellulose nanofiber (CNF) architectures formed in the obtained hydrogels under UV irradiation, where CNFs themselves serve as both initiators and cross-linkers.
34787399	2	11	theme	wood	269:272	arg1	wall					279:282	the wood cell wall	265:282	the wood cell wall	265:282	The hypothesis is that improved interfacial interaction between the wood cell wall and a polymer is of great importance for improving the mechanical performance.
34787399	3	12	theme	-infiltrated	480:491	arg1	skeleton					498:505	the polyacrylamide (PAM)-infiltrated wood skeleton	456:505	the polyacrylamide (PAM)-infiltrated wood skeleton	456:505	To this end, a facile and green approach, called ultraviolet (UV) grafting, was performed on the polyacrylamide (PAM)-infiltrated wood skeleton without using initiators.
34787399	3	13	theme	UV	425:426	arg1	grafting					429:436	ultraviolet (UV) grafting	412:436	ultraviolet (UV) grafting	412:436	To this end, a facile and green approach, called ultraviolet (UV) grafting, was performed on the polyacrylamide (PAM)-infiltrated wood skeleton without using initiators.
34787399	5	14	theme	alkali	749:754	arg1	treatment					765:773	an alkali swelling treatment	746:773	an alkali swelling treatment	746:773	Moreover, an alkali swelling treatment was utilized to improve the accessibility of the wood cell wall before UV irradiation and thus facilitate grafting efficiency.
34787399	3	15	theme	PAM	476:478	arg1	skeleton					498:505	the polyacrylamide (PAM)-infiltrated wood skeleton	456:505	the polyacrylamide (PAM)-infiltrated wood skeleton	456:505	To this end, a facile and green approach, called ultraviolet (UV) grafting, was performed on the polyacrylamide (PAM)-infiltrated wood skeleton without using initiators.
34787399	0	16	theme	Strong	7:12	arg1	Hydrogels					35:43	Strong and Tough Wood-Based Hydrogels	7:43	Strong and Tough Wood-Based Hydrogels for Sensors	7:55	Toward Strong and Tough Wood-Based Hydrogels for Sensors.
34787399	6	17	theme	resulting	906:914	arg1	hydrogels					942:950	The resulting alkali-treated Wood-g-PAM hydrogels	902:950	The resulting alkali-treated Wood-g-PAM hydrogels	902:950	The resulting alkali-treated Wood-g-PAM hydrogels exhibited significantly higher tensile properties than those of the Wood/PAM hydrogel and were further assembled into conductive devices for sensor applications.
34787399	5	18	theme	grafting	881:888	arg1	efficiency					890:899	grafting efficiency	881:899	grafting efficiency	881:899	Moreover, an alkali swelling treatment was utilized to improve the accessibility of the wood cell wall before UV irradiation and thus facilitate grafting efficiency.
34787399	5	19	theme	swelling	756:763	arg1	treatment					765:773	an alkali swelling treatment	746:773	an alkali swelling treatment	746:773	Moreover, an alkali swelling treatment was utilized to improve the accessibility of the wood cell wall before UV irradiation and thus facilitate grafting efficiency.
34787399	7	20	theme	grafting	1138:1145	arg1	strategy					1147:1154	this UV grafting strategy	1130:1154	this UV grafting strategy	1130:1154	We believe that this UV grafting strategy may facilitate the development of strong wood-based composites with interesting features.
34787399	1	21	theme	research	78:85	arg1	purpose					62:68	The purpose	58:68	The purpose of this research	58:85	The purpose of this research is to develop strong and tough wood-based hydrogels, which are reinforced by an aligned cellulosic wood skeleton.
34787399	3	22	theme	green	389:393	arg1	approach					395:402	a facile and green approach	376:402	a facile and green approach	376:402	To this end, a facile and green approach, called ultraviolet (UV) grafting, was performed on the polyacrylamide (PAM)-infiltrated wood skeleton without using initiators.
34787399	0	23	theme	Tough	18:22	arg1	Hydrogels					35:43	Strong and Tough Wood-Based Hydrogels	7:43	Strong and Tough Wood-Based Hydrogels for Sensors	7:55	Toward Strong and Tough Wood-Based Hydrogels for Sensors.
34787399	6	24	theme	Wood-g-PAM	931:940	arg1	hydrogels					942:950	The resulting alkali-treated Wood-g-PAM hydrogels	902:950	The resulting alkali-treated Wood-g-PAM hydrogels	902:950	The resulting alkali-treated Wood-g-PAM hydrogels exhibited significantly higher tensile properties than those of the Wood/PAM hydrogel and were further assembled into conductive devices for sensor applications.
34787399	3	25	theme	wood	493:496	arg1	skeleton					498:505	the polyacrylamide (PAM)-infiltrated wood skeleton	456:505	the polyacrylamide (PAM)-infiltrated wood skeleton	456:505	To this end, a facile and green approach, called ultraviolet (UV) grafting, was performed on the polyacrylamide (PAM)-infiltrated wood skeleton without using initiators.
34787399	1	26	theme	aligned	167:173	arg1	skeleton					191:198	an aligned cellulosic wood skeleton	164:198	an aligned cellulosic wood skeleton	164:198	The purpose of this research is to develop strong and tough wood-based hydrogels, which are reinforced by an aligned cellulosic wood skeleton.
34787399	6	27	theme	alkali-treated	916:929	arg1	hydrogels					942:950	The resulting alkali-treated Wood-g-PAM hydrogels	902:950	The resulting alkali-treated Wood-g-PAM hydrogels	902:950	The resulting alkali-treated Wood-g-PAM hydrogels exhibited significantly higher tensile properties than those of the Wood/PAM hydrogel and were further assembled into conductive devices for sensor applications.
34787399	6	28	theme	conductive	1070:1079	arg1	devices					1081:1087	conductive devices	1070:1087	conductive devices for sensor applications	1070:1111	The resulting alkali-treated Wood-g-PAM hydrogels exhibited significantly higher tensile properties than those of the Wood/PAM hydrogel and were further assembled into conductive devices for sensor applications.
34787399	1	29	theme	cellulosic	175:184	arg1	skeleton					191:198	an aligned cellulosic wood skeleton	164:198	an aligned cellulosic wood skeleton	164:198	The purpose of this research is to develop strong and tough wood-based hydrogels, which are reinforced by an aligned cellulosic wood skeleton.
34787399	7	30	theme	UV	1135:1136	arg1	strategy					1147:1154	this UV grafting strategy	1130:1154	this UV grafting strategy	1130:1154	We believe that this UV grafting strategy may facilitate the development of strong wood-based composites with interesting features.
34787399	2	31	theme	great	304:308	arg1	importance					310:319	great importance	304:319	great importance	304:319	The hypothesis is that improved interfacial interaction between the wood cell wall and a polymer is of great importance for improving the mechanical performance.
34787399	6	32	theme	Wood/PAM	1020:1027	arg1	hydrogel					1029:1036	the Wood/PAM hydrogel	1016:1036	the Wood/PAM hydrogel	1016:1036	The resulting alkali-treated Wood-g-PAM hydrogels exhibited significantly higher tensile properties than those of the Wood/PAM hydrogel and were further assembled into conductive devices for sensor applications.
34787399	5	33	theme	wood	824:827	arg1	wall					834:837	the wood cell wall	820:837	the wood cell wall	820:837	Moreover, an alkali swelling treatment was utilized to improve the accessibility of the wood cell wall before UV irradiation and thus facilitate grafting efficiency.
34787399	1	34	theme	wood	186:189	arg1	skeleton					191:198	an aligned cellulosic wood skeleton	164:198	an aligned cellulosic wood skeleton	164:198	The purpose of this research is to develop strong and tough wood-based hydrogels, which are reinforced by an aligned cellulosic wood skeleton.
34787399	0	35	theme	Wood-Based	24:33	arg1	Hydrogels					35:43	Strong and Tough Wood-Based Hydrogels	7:43	Strong and Tough Wood-Based Hydrogels for Sensors	7:55	Toward Strong and Tough Wood-Based Hydrogels for Sensors.
34787399	3	36	theme	facile	378:383	arg1	approach					395:402	a facile and green approach	376:402	a facile and green approach	376:402	To this end, a facile and green approach, called ultraviolet (UV) grafting, was performed on the polyacrylamide (PAM)-infiltrated wood skeleton without using initiators.
34787399	3	37	theme	ultraviolet	412:422	arg1	grafting					429:436	ultraviolet (UV) grafting	412:436	ultraviolet (UV) grafting	412:436	To this end, a facile and green approach, called ultraviolet (UV) grafting, was performed on the polyacrylamide (PAM)-infiltrated wood skeleton without using initiators.
34787399	5	38	theme	cell	829:832	arg1	wall					834:837	the wood cell wall	820:837	the wood cell wall	820:837	Moreover, an alkali swelling treatment was utilized to improve the accessibility of the wood cell wall before UV irradiation and thus facilitate grafting efficiency.
34787399	6	39	theme	sensor	1093:1098	arg1	applications					1100:1111	sensor applications	1093:1111	sensor applications	1093:1111	The resulting alkali-treated Wood-g-PAM hydrogels exhibited significantly higher tensile properties than those of the Wood/PAM hydrogel and were further assembled into conductive devices for sensor applications.
34787399	1	40	theme	strong	101:106	arg1	hydrogels					129:137	strong and tough wood-based hydrogels	101:137	strong and tough wood-based hydrogels	101:137	The purpose of this research is to develop strong and tough wood-based hydrogels, which are reinforced by an aligned cellulosic wood skeleton.
34787399	1	41	theme	wood-based	118:127	arg1	hydrogels					129:137	strong and tough wood-based hydrogels	101:137	strong and tough wood-based hydrogels	101:137	The purpose of this research is to develop strong and tough wood-based hydrogels, which are reinforced by an aligned cellulosic wood skeleton.
34787399	5	42	dep	improve	791:797	arg1	facilitate					870:879	facilitate	870:879	facilitate grafting efficiency	870:899	Moreover, an alkali swelling treatment was utilized to improve the accessibility of the wood cell wall before UV irradiation and thus facilitate grafting efficiency.
34787399	5	43	used	utilized	779:786	arg2	treatment					765:773	an alkali swelling treatment	746:773	an alkali swelling treatment	746:773	Moreover, an alkali swelling treatment was utilized to improve the accessibility of the wood cell wall before UV irradiation and thus facilitate grafting efficiency.
34787399	2	44	theme	interfacial	233:243	arg1	interaction					245:255	improved interfacial interaction	224:255	improved interfacial interaction between the wood cell wall and a polymer	224:296	The hypothesis is that improved interfacial interaction between the wood cell wall and a polymer is of great importance for improving the mechanical performance.
34787399	1	45	theme	tough	112:116	arg1	hydrogels					129:137	strong and tough wood-based hydrogels	101:137	strong and tough wood-based hydrogels	101:137	The purpose of this research is to develop strong and tough wood-based hydrogels, which are reinforced by an aligned cellulosic wood skeleton.
34787399	7	46	theme	interesting	1224:1234	arg1	features					1236:1243	interesting features	1224:1243	interesting features	1224:1243	We believe that this UV grafting strategy may facilitate the development of strong wood-based composites with interesting features.
34787399	5	47	theme	wall	834:837	arg1	accessibility					803:815	the accessibility	799:815	the accessibility of the wood cell wall before UV irradiation	799:859	Moreover, an alkali swelling treatment was utilized to improve the accessibility of the wood cell wall before UV irradiation and thus facilitate grafting efficiency.
34787399	2	48	theme	improved	224:231	arg1	interaction					245:255	improved interfacial interaction	224:255	improved interfacial interaction between the wood cell wall and a polymer	224:296	The hypothesis is that improved interfacial interaction between the wood cell wall and a polymer is of great importance for improving the mechanical performance.
34787399	4	49	theme	nanofiber	585:593	arg1	architectures					601:613	PAM-grafted cellulose nanofiber (CNF) architectures	563:613	PAM-grafted cellulose nanofiber (CNF) architectures	563:613	An important finding was that PAM-grafted cellulose nanofiber (CNF) architectures formed in the obtained hydrogels under UV irradiation, where CNFs themselves serve as both initiators and cross-linkers.
34787399	2	50	theme	mechanical	339:348	arg1	performance					350:360	the mechanical performance	335:360	the mechanical performance	335:360	The hypothesis is that improved interfacial interaction between the wood cell wall and a polymer is of great importance for improving the mechanical performance.
34787399	4	51	theme	PAM-grafted	563:573	arg1	CNF					596:598	CNF	596:598	CNF	596:598	An important finding was that PAM-grafted cellulose nanofiber (CNF) architectures formed in the obtained hydrogels under UV irradiation, where CNFs themselves serve as both initiators and cross-linkers.
34787399	4	51	theme	PAM-grafted	563:573	arg1	nanofiber					585:593	PAM-grafted cellulose nanofiber	563:593	PAM-grafted cellulose nanofiber (CNF) architectures	563:613	An important finding was that PAM-grafted cellulose nanofiber (CNF) architectures formed in the obtained hydrogels under UV irradiation, where CNFs themselves serve as both initiators and cross-linkers.
34787399	3	52	theme	polyacrylamide	460:473	arg1	skeleton					498:505	the polyacrylamide (PAM)-infiltrated wood skeleton	456:505	the polyacrylamide (PAM)-infiltrated wood skeleton	456:505	To this end, a facile and green approach, called ultraviolet (UV) grafting, was performed on the polyacrylamide (PAM)-infiltrated wood skeleton without using initiators.
34787399	4	53	theme	UV	654:655	arg1	irradiation					657:667	UV irradiation	654:667	UV irradiation	654:667	An important finding was that PAM-grafted cellulose nanofiber (CNF) architectures formed in the obtained hydrogels under UV irradiation, where CNFs themselves serve as both initiators and cross-linkers.
32454440	5	0	theme	lower	1267:1271	arg1	concentration					1278:1290	lower NaOH concentration	1267:1290	lower NaOH concentration	1267:1290	In conclusion, mechanical fragmentation contributes to cellulose separation and cellulose crystalline transformation under lower NaOH concentration.
32454440	1	1	from	effects	187:193	arg1	separation					246:255	cellulose separation	236:255	cellulose separation	236:255	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	1	1	from	effects	187:193	arg1	transformation					283:296	cellulose polymorphic transformation	261:296	cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%)	261:473	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	4	2	theme	BM120	1105:1109	arg1	transformation					1074:1087	cellulose crystalline transformation	1052:1087	cellulose crystalline transformation of CM, BM30 and BM120	1052:1109	X-ray diffraction results reveals that the NaOH concentration required for cellulose crystalline transformation of CM, BM30 and BM120 is 10%, 8% and 2%, respectively.
32454440	3	3	theme	separation	849:858	arg1	effect					829:834	The enhancement effect	813:834	The enhancement effect of cellulose separation with the increasing mechanical fragmentation intensity	813:913	The enhancement effect of cellulose separation with the increasing mechanical fragmentation intensity is mainly due to the increasing hemicellulose solubilization.
32454440	3	3	theme	separation	849:858	arg1	due					925:927	due	925:927	due	925:927	The enhancement effect of cellulose separation with the increasing mechanical fragmentation intensity is mainly due to the increasing hemicellulose solubilization.
32454440	0	4	theme	cellulosic	86:95	arg1	variation					124:132	cellulosic and crystalline-structural variation	86:132	cellulosic and crystalline-structural variation of NaOH	86:140	Quantitative and qualitative characterization of dual scale mechanical enhancement on cellulosic and crystalline-structural variation of NaOH treated wheat straw.
32454440	1	5	dep	transformation	283:296	arg1	treated					423:429	treated	423:429	were treated with different NaOH concentrations (1%-10%)	418:473	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	2	6	theme	mechanical	686:695	arg1	samples					705:711	different mechanical treated samples	676:711	different mechanical treated samples	676:711	The quantitative equations between cellulose content and NaOH concentration of different mechanical treated samples were YCM = 69.8-35.1exp(-0.64X)), YBM30 = 71.3-35.1exp(-0.86X)) and YBM120 = 73.5-35.1exp(-1.82X)).
32454440	2	7	theme	samples	705:711	arg1	content					642:648	cellulose content	632:648	cellulose content	632:648	The quantitative equations between cellulose content and NaOH concentration of different mechanical treated samples were YCM = 69.8-35.1exp(-0.64X)), YBM30 = 71.3-35.1exp(-0.86X)) and YBM120 = 73.5-35.1exp(-1.82X)).
32454440	2	7	theme	samples	705:711	arg1	concentration					659:671	NaOH concentration	654:671	NaOH concentration	654:671	The quantitative equations between cellulose content and NaOH concentration of different mechanical treated samples were YCM = 69.8-35.1exp(-0.64X)), YBM30 = 71.3-35.1exp(-0.86X)) and YBM120 = 73.5-35.1exp(-1.82X)).
32454440	2	8	theme	quantitative	601:612	arg1	equations					614:622	The quantitative equations	597:622	The quantitative equations between cellulose content and NaOH concentration of different mechanical treated samples	597:711	The quantitative equations between cellulose content and NaOH concentration of different mechanical treated samples were YCM = 69.8-35.1exp(-0.64X)), YBM30 = 71.3-35.1exp(-0.86X)) and YBM120 = 73.5-35.1exp(-1.82X)).
32454440	2	8	theme	quantitative	601:612	arg1	YCM = 69.8-35.1exp					718:735	YCM = 69.8-35.1exp	718:735	YCM = 69.8-35.1exp(-0.64X))	718:744	The quantitative equations between cellulose content and NaOH concentration of different mechanical treated samples were YCM = 69.8-35.1exp(-0.64X)), YBM30 = 71.3-35.1exp(-0.86X)) and YBM120 = 73.5-35.1exp(-1.82X)).
32454440	1	9	theme	different	436:444	arg1	%					472:472	1%-10%	467:472	1%-10%	467:472	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	1	9	theme	different	436:444	arg1	concentrations					451:464	different NaOH concentrations	436:464	different NaOH concentrations (1%-10%)	436:473	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	3	10	with	effect	829:834	arg1	intensity					905:913	the increasing mechanical fragmentation intensity	865:913	the increasing mechanical fragmentation intensity	865:913	The enhancement effect of cellulose separation with the increasing mechanical fragmentation intensity is mainly due to the increasing hemicellulose solubilization.
32454440	1	11	theme	wheat	301:305	arg1	straw					307:311	wheat straw	301:311	wheat straw	301:311	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	0	12	theme	crystalline-structural	101:122	arg1	variation					124:132	cellulosic and crystalline-structural variation	86:132	cellulosic and crystalline-structural variation of NaOH	86:140	Quantitative and qualitative characterization of dual scale mechanical enhancement on cellulosic and crystalline-structural variation of NaOH treated wheat straw.
32454440	1	13	theme	ball	369:372	arg1	BM30					402:405	BM30	402:405	BM30	402:405	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	1	13	theme	ball	369:372	arg1	samples					393:399	two ball milled wheat straw samples	365:399	two ball milled wheat straw samples (BM30 and BM120)	365:416	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	1	13	theme	ball	369:372	arg1	BM120					411:415	BM120	411:415	BM120	411:415	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	1	14	theme	NaOH	446:449	arg1	%					472:472	1%-10%	467:472	1%-10%	467:472	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	1	14	theme	NaOH	446:449	arg1	concentrations					451:464	different NaOH concentrations	436:464	different NaOH concentrations (1%-10%)	436:473	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	3	15	theme	increasing	936:945	arg1	solubilization					961:974	the increasing hemicellulose solubilization	932:974	the increasing hemicellulose solubilization	932:974	The enhancement effect of cellulose separation with the increasing mechanical fragmentation intensity is mainly due to the increasing hemicellulose solubilization.
32454440	1	16	theme	straw	307:311	arg1	separation					246:255	cellulose separation	236:255	cellulose separation	236:255	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	1	16	theme	straw	307:311	arg1	transformation					283:296	cellulose polymorphic transformation	261:296	cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%)	261:473	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	0	17	theme	NaOH	137:140	arg1	variation					124:132	cellulosic and crystalline-structural variation	86:132	cellulosic and crystalline-structural variation of NaOH	86:140	Quantitative and qualitative characterization of dual scale mechanical enhancement on cellulosic and crystalline-structural variation of NaOH treated wheat straw.
32454440	1	18	theme	milled	374:379	arg1	BM30					402:405	BM30	402:405	BM30	402:405	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	1	18	theme	milled	374:379	arg1	samples					393:399	two ball milled wheat straw samples	365:399	two ball milled wheat straw samples (BM30 and BM120)	365:416	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	1	18	theme	milled	374:379	arg1	BM120					411:415	BM120	411:415	BM120	411:415	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	2	19	theme	NaOH	654:657	arg1	concentration					659:671	NaOH concentration	654:671	NaOH concentration	654:671	The quantitative equations between cellulose content and NaOH concentration of different mechanical treated samples were YCM = 69.8-35.1exp(-0.64X)), YBM30 = 71.3-35.1exp(-0.86X)) and YBM120 = 73.5-35.1exp(-1.82X)).
32454440	3	20	theme	hemicellulose	947:959	arg1	solubilization					961:974	the increasing hemicellulose solubilization	932:974	the increasing hemicellulose solubilization	932:974	The enhancement effect of cellulose separation with the increasing mechanical fragmentation intensity is mainly due to the increasing hemicellulose solubilization.
32454440	3	21	theme	cellulose	839:847	arg1	separation					849:858	cellulose separation	839:858	cellulose separation	839:858	The enhancement effect of cellulose separation with the increasing mechanical fragmentation intensity is mainly due to the increasing hemicellulose solubilization.
32454440	1	22	theme	wheat	381:385	arg1	BM30					402:405	BM30	402:405	BM30	402:405	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	1	22	theme	wheat	381:385	arg1	samples					393:399	two ball milled wheat straw samples	365:399	two ball milled wheat straw samples (BM30 and BM120)	365:416	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	1	22	theme	wheat	381:385	arg1	BM120					411:415	BM120	411:415	BM120	411:415	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	4	23	theme	crystalline	1062:1072	arg1	transformation					1074:1087	cellulose crystalline transformation	1052:1087	cellulose crystalline transformation of CM, BM30 and BM120	1052:1109	X-ray diffraction results reveals that the NaOH concentration required for cellulose crystalline transformation of CM, BM30 and BM120 is 10%, 8% and 2%, respectively.
32454440	4	24	theme	CM	1092:1093	arg1	transformation					1074:1087	cellulose crystalline transformation	1052:1087	cellulose crystalline transformation of CM, BM30 and BM120	1052:1109	X-ray diffraction results reveals that the NaOH concentration required for cellulose crystalline transformation of CM, BM30 and BM120 is 10%, 8% and 2%, respectively.
32454440	1	25	theme	straw	387:391	arg1	BM30					402:405	BM30	402:405	BM30	402:405	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	1	25	theme	straw	387:391	arg1	samples					393:399	two ball milled wheat straw samples	365:399	two ball milled wheat straw samples (BM30 and BM120)	365:416	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	1	25	theme	straw	387:391	arg1	BM120					411:415	BM120	411:415	BM120	411:415	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	0	26	theme	Quantitative	0:11	arg1	characterization					29:44	Quantitative and qualitative characterization	0:44	Quantitative and qualitative characterization of dual scale mechanical enhancement on cellulosic and crystalline-structural variation of NaOH	0:140	Quantitative and qualitative characterization of dual scale mechanical enhancement on cellulosic and crystalline-structural variation of NaOH treated wheat straw.
32454440	5	27	theme	cellulose	1199:1207	arg1	separation					1209:1218	cellulose separation	1199:1218	cellulose separation	1199:1218	In conclusion, mechanical fragmentation contributes to cellulose separation and cellulose crystalline transformation under lower NaOH concentration.
32454440	2	28	theme	cellulose	632:640	arg1	content					642:648	cellulose content	632:648	cellulose content	632:648	The quantitative equations between cellulose content and NaOH concentration of different mechanical treated samples were YCM = 69.8-35.1exp(-0.64X)), YBM30 = 71.3-35.1exp(-0.86X)) and YBM120 = 73.5-35.1exp(-1.82X)).
32454440	1	29	theme	%	468:468	arg1	%					472:472	1%-10%	467:472	1%-10%	467:472	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	1	29	theme	%	468:468	arg1	concentrations					451:464	different NaOH concentrations	436:464	different NaOH concentrations (1%-10%)	436:473	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	1	30	theme	different	198:206	arg1	fragmentation					219:231	different mechanical fragmentation	198:231	different mechanical fragmentation	198:231	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	1	31	theme	alkali	320:325	arg1	treatment					327:335	alkali treatment	320:335	alkali treatment	320:335	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	0	32	theme	wheat	150:154	arg1	straw					156:160	wheat straw	150:160	wheat straw	150:160	Quantitative and qualitative characterization of dual scale mechanical enhancement on cellulosic and crystalline-structural variation of NaOH treated wheat straw.
32454440	2	33	theme	treated	697:703	arg1	samples					705:711	different mechanical treated samples	676:711	different mechanical treated samples	676:711	The quantitative equations between cellulose content and NaOH concentration of different mechanical treated samples were YCM = 69.8-35.1exp(-0.64X)), YBM30 = 71.3-35.1exp(-0.86X)) and YBM120 = 73.5-35.1exp(-1.82X)).
32454440	1	34	theme	mechanical	208:217	arg1	fragmentation					219:231	different mechanical fragmentation	198:231	different mechanical fragmentation	198:231	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	0	35	theme	qualitative	17:27	arg1	characterization					29:44	Quantitative and qualitative characterization	0:44	Quantitative and qualitative characterization of dual scale mechanical enhancement on cellulosic and crystalline-structural variation of NaOH	0:140	Quantitative and qualitative characterization of dual scale mechanical enhancement on cellulosic and crystalline-structural variation of NaOH treated wheat straw.
32454440	1	36	theme	fragmentation	219:231	arg1	effects					187:193	the effects	183:193	the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%)	183:473	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	0	37	theme	dual	49:52	arg1	enhancement					71:81	dual scale mechanical enhancement	49:81	dual scale mechanical enhancement on cellulosic and crystalline-structural variation of NaOH	49:140	Quantitative and qualitative characterization of dual scale mechanical enhancement on cellulosic and crystalline-structural variation of NaOH treated wheat straw.
32454440	5	38	theme	cellulose	1224:1232	arg1	transformation					1246:1259	cellulose crystalline transformation	1224:1259	cellulose crystalline transformation	1224:1259	In conclusion, mechanical fragmentation contributes to cellulose separation and cellulose crystalline transformation under lower NaOH concentration.
32454440	4	39	theme	diffraction	983:993	arg1	results					995:1001	X-ray diffraction results	977:1001	X-ray diffraction results	977:1001	X-ray diffraction results reveals that the NaOH concentration required for cellulose crystalline transformation of CM, BM30 and BM120 is 10%, 8% and 2%, respectively.
32454440	4	40	theme	cellulose	1052:1060	arg1	transformation					1074:1087	cellulose crystalline transformation	1052:1087	cellulose crystalline transformation of CM, BM30 and BM120	1052:1109	X-ray diffraction results reveals that the NaOH concentration required for cellulose crystalline transformation of CM, BM30 and BM120 is 10%, 8% and 2%, respectively.
32454440	5	41	theme	mechanical	1159:1168	arg1	fragmentation					1170:1182	mechanical fragmentation	1159:1182	mechanical fragmentation	1159:1182	In conclusion, mechanical fragmentation contributes to cellulose separation and cellulose crystalline transformation under lower NaOH concentration.
32454440	4	42	theme	X-ray	977:981	arg1	results					995:1001	X-ray diffraction results	977:1001	X-ray diffraction results	977:1001	X-ray diffraction results reveals that the NaOH concentration required for cellulose crystalline transformation of CM, BM30 and BM120 is 10%, 8% and 2%, respectively.
32454440	1	43	dep	samples	393:399	arg1	BM30					402:405	BM30	402:405	BM30	402:405	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	1	43	dep	samples	393:399	arg1	samples					393:399	two ball milled wheat straw samples	365:399	two ball milled wheat straw samples (BM30 and BM120)	365:416	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	1	43	dep	samples	393:399	arg1	BM120					411:415	BM120	411:415	BM120	411:415	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	1	44	theme	cellulose	236:244	arg1	separation					246:255	cellulose separation	236:255	cellulose separation	236:255	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	0	45	theme	mechanical	60:69	arg1	enhancement					71:81	dual scale mechanical enhancement	49:81	dual scale mechanical enhancement on cellulosic and crystalline-structural variation of NaOH	49:140	Quantitative and qualitative characterization of dual scale mechanical enhancement on cellulosic and crystalline-structural variation of NaOH treated wheat straw.
32454440	0	46	from	enhancement	71:81	arg1	variation					124:132	cellulosic and crystalline-structural variation	86:132	cellulosic and crystalline-structural variation of NaOH	86:140	Quantitative and qualitative characterization of dual scale mechanical enhancement on cellulosic and crystalline-structural variation of NaOH treated wheat straw.
32454440	0	47	theme	scale	54:58	arg1	enhancement					71:81	dual scale mechanical enhancement	49:81	dual scale mechanical enhancement on cellulosic and crystalline-structural variation of NaOH	49:140	Quantitative and qualitative characterization of dual scale mechanical enhancement on cellulosic and crystalline-structural variation of NaOH treated wheat straw.
32454440	4	48	theme	NaOH	1020:1023	arg1	concentration					1025:1037	the NaOH concentration	1016:1037	the NaOH concentration required for cellulose crystalline transformation of CM, BM30 and BM120	1016:1109	X-ray diffraction results reveals that the NaOH concentration required for cellulose crystalline transformation of CM, BM30 and BM120 is 10%, 8% and 2%, respectively.
32454440	4	48	theme	NaOH	1020:1023	arg1	%					1116:1116	10%	1114:1116	10%	1114:1116	X-ray diffraction results reveals that the NaOH concentration required for cellulose crystalline transformation of CM, BM30 and BM120 is 10%, 8% and 2%, respectively.
32454440	3	49	theme	mechanical	880:889	arg1	intensity					905:913	the increasing mechanical fragmentation intensity	865:913	the increasing mechanical fragmentation intensity	865:913	The enhancement effect of cellulose separation with the increasing mechanical fragmentation intensity is mainly due to the increasing hemicellulose solubilization.
32454440	2	50	theme	different	676:684	arg1	samples					705:711	different mechanical treated samples	676:711	different mechanical treated samples	676:711	The quantitative equations between cellulose content and NaOH concentration of different mechanical treated samples were YCM = 69.8-35.1exp(-0.64X)), YBM30 = 71.3-35.1exp(-0.86X)) and YBM120 = 73.5-35.1exp(-1.82X)).
32454440	3	51	theme	fragmentation	891:903	arg1	intensity					905:913	the increasing mechanical fragmentation intensity	865:913	the increasing mechanical fragmentation intensity	865:913	The enhancement effect of cellulose separation with the increasing mechanical fragmentation intensity is mainly due to the increasing hemicellulose solubilization.
32454440	3	52	theme	enhancement	817:827	arg1	effect					829:834	The enhancement effect	813:834	The enhancement effect of cellulose separation with the increasing mechanical fragmentation intensity	813:913	The enhancement effect of cellulose separation with the increasing mechanical fragmentation intensity is mainly due to the increasing hemicellulose solubilization.
32454440	3	52	theme	enhancement	817:827	arg1	due					925:927	due	925:927	due	925:927	The enhancement effect of cellulose separation with the increasing mechanical fragmentation intensity is mainly due to the increasing hemicellulose solubilization.
32454440	0	53	from	variation	124:132	arg1	characterization					29:44	Quantitative and qualitative characterization	0:44	Quantitative and qualitative characterization of dual scale mechanical enhancement on cellulosic and crystalline-structural variation of NaOH	0:140	Quantitative and qualitative characterization of dual scale mechanical enhancement on cellulosic and crystalline-structural variation of NaOH treated wheat straw.
32454440	5	54	theme	crystalline	1234:1244	arg1	transformation					1246:1259	cellulose crystalline transformation	1224:1259	cellulose crystalline transformation	1224:1259	In conclusion, mechanical fragmentation contributes to cellulose separation and cellulose crystalline transformation under lower NaOH concentration.
32454440	1	55	theme	lignocellulosic	484:498	arg1	compositions					500:511	the lignocellulosic compositions	480:511	the lignocellulosic compositions	480:511	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	3	56	theme	increasing	869:878	arg1	intensity					905:913	the increasing mechanical fragmentation intensity	865:913	the increasing mechanical fragmentation intensity	865:913	The enhancement effect of cellulose separation with the increasing mechanical fragmentation intensity is mainly due to the increasing hemicellulose solubilization.
32454440	1	57	theme	cellulose	261:269	arg1	transformation					283:296	cellulose polymorphic transformation	261:296	cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%)	261:473	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32454440	0	58	theme	enhancement	71:81	arg1	characterization					29:44	Quantitative and qualitative characterization	0:44	Quantitative and qualitative characterization of dual scale mechanical enhancement on cellulosic and crystalline-structural variation of NaOH	0:140	Quantitative and qualitative characterization of dual scale mechanical enhancement on cellulosic and crystalline-structural variation of NaOH treated wheat straw.
32454440	0	59	from	characterization	29:44	arg1	variation					124:132	cellulosic and crystalline-structural variation	86:132	cellulosic and crystalline-structural variation of NaOH	86:140	Quantitative and qualitative characterization of dual scale mechanical enhancement on cellulosic and crystalline-structural variation of NaOH treated wheat straw.
32454440	4	60	theme	BM30	1096:1099	arg1	transformation					1074:1087	cellulose crystalline transformation	1052:1087	cellulose crystalline transformation of CM, BM30 and BM120	1052:1109	X-ray diffraction results reveals that the NaOH concentration required for cellulose crystalline transformation of CM, BM30 and BM120 is 10%, 8% and 2%, respectively.
32454440	5	61	theme	NaOH	1273:1276	arg1	concentration					1278:1290	lower NaOH concentration	1267:1290	lower NaOH concentration	1267:1290	In conclusion, mechanical fragmentation contributes to cellulose separation and cellulose crystalline transformation under lower NaOH concentration.
32454440	1	62	theme	polymorphic	271:281	arg1	transformation					283:296	cellulose polymorphic transformation	261:296	cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%)	261:473	In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and the lignocellulosic compositions and crystalline-structural various were quantitative and qualitative characterized.
32173991	0	0	theme	microRNA	100:107	arg1	detection					109:117	microRNA detection	100:117	microRNA detection using magnetic separation	100:143	A highly sensitive fluorescence sensor based on lucigenin/chitosan/SiO2 composite nanoparticles for microRNA detection using magnetic separation.
32173991	7	1	theme	lucigenin/chitosan/SiO2	1546:1568	arg1	indicator					1610:1618	a DNA hybrid indicator	1597:1618	a DNA hybrid indicator	1597:1618	Therefore, a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free and no immobilization was established using lucigenin/chitosan/SiO2 composite nanoparticles as a DNA hybrid indicator.
32173991	7	1	theme	lucigenin/chitosan/SiO2	1546:1568	arg1	nanoparticles					1580:1592	lucigenin/chitosan/SiO2 composite nanoparticles	1546:1592	lucigenin/chitosan/SiO2 composite nanoparticles	1546:1592	Therefore, a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free and no immobilization was established using lucigenin/chitosan/SiO2 composite nanoparticles as a DNA hybrid indicator.
32173991	1	2	theme	convenient	163:172	arg1	method					202:207	a convenient reverse-phase microemulsion method	161:207	a convenient reverse-phase microemulsion method for the synthesis of SiO2 nanoparticles (NPs) by simply introducing the chitosan and fluorescent dye of lucigenin during the formation reaction of SiO2 NPs	161:363	In this paper, a convenient reverse-phase microemulsion method for the synthesis of SiO2 nanoparticles (NPs) by simply introducing the chitosan and fluorescent dye of lucigenin during the formation reaction of SiO2 NPs was proposed.
32173991	2	3	theme	NPs	496:498	arg1	nanopores					500:508	chitosan/SiO2 NPs nanopores	482:508	chitosan/SiO2 NPs nanopores based on electrostatic interaction and supermolecular forces	482:569	Addition of chitosan can make the SiO2 NPs porous, and increases lucigenin molecule incorporation into chitosan/SiO2 NPs nanopores based on electrostatic interaction and supermolecular forces.
32173991	1	4	theme	SiO2	356:359	arg1	NPs					361:363	SiO2 NPs	356:363	SiO2 NPs	356:363	In this paper, a convenient reverse-phase microemulsion method for the synthesis of SiO2 nanoparticles (NPs) by simply introducing the chitosan and fluorescent dye of lucigenin during the formation reaction of SiO2 NPs was proposed.
32173991	1	5	theme	reverse-phase	174:186	arg1	method					202:207	a convenient reverse-phase microemulsion method	161:207	a convenient reverse-phase microemulsion method for the synthesis of SiO2 nanoparticles (NPs) by simply introducing the chitosan and fluorescent dye of lucigenin during the formation reaction of SiO2 NPs	161:363	In this paper, a convenient reverse-phase microemulsion method for the synthesis of SiO2 nanoparticles (NPs) by simply introducing the chitosan and fluorescent dye of lucigenin during the formation reaction of SiO2 NPs was proposed.
32173991	8	6	theme	high	1645:1648	arg1	sensitivity					1650:1660	high sensitivity	1645:1660	high sensitivity	1645:1660	The proposed method had high sensitivity and selectivity, low cost and the detection limit was 10 fM (S/N = 3).
32173991	2	7	theme	electrostatic	519:531	arg1	interaction					533:543	electrostatic interaction	519:543	electrostatic interaction	519:543	Addition of chitosan can make the SiO2 NPs porous, and increases lucigenin molecule incorporation into chitosan/SiO2 NPs nanopores based on electrostatic interaction and supermolecular forces.
32173991	2	8	theme	supermolecular	549:562	arg1	forces					564:569	supermolecular forces	549:569	supermolecular forces	549:569	Addition of chitosan can make the SiO2 NPs porous, and increases lucigenin molecule incorporation into chitosan/SiO2 NPs nanopores based on electrostatic interaction and supermolecular forces.
32173991	1	9	theme	NPs	361:363	arg1	reaction					344:351	the formation reaction	330:351	the formation reaction of SiO2 NPs	330:363	In this paper, a convenient reverse-phase microemulsion method for the synthesis of SiO2 nanoparticles (NPs) by simply introducing the chitosan and fluorescent dye of lucigenin during the formation reaction of SiO2 NPs was proposed.
32173991	4	10	theme	composite	943:951	arg1	nanoparticles					953:965	lucigenin/chitosan/SiO2 composite nanoparticles	919:965	lucigenin/chitosan/SiO2 composite nanoparticles with positive charge adsorbed using ssDNA or dsDNA	919:1016	Because the number of negative charges carried when using single-stranded DNA (ssDNA) was different from that of double-stranded DNA (dsDNA), the numbers of lucigenin/chitosan/SiO2 composite nanoparticles with positive charge adsorbed using ssDNA or dsDNA were different.
32173991	6	11	theme	fluorescence	1287:1298	arg1	intensity					1300:1308	fluorescence intensity	1287:1308	fluorescence intensity sensitizing value and target miRNA concentrations	1287:1358	With increase in target DNA/miRNA concentration, the difference in fluorescence intensity also increased, resulting in a good linear relationship between fluorescence intensity sensitizing value and target miRNA concentrations.
32173991	7	12	theme	human	1441:1445	arg1	samples					1467:1473	human gastric cancer cell samples	1441:1473	human gastric cancer cell samples	1441:1473	Therefore, a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free and no immobilization was established using lucigenin/chitosan/SiO2 composite nanoparticles as a DNA hybrid indicator.
32173991	6	13	from	difference	1186:1195	arg1	intensity					1213:1221	fluorescence intensity	1200:1221	fluorescence intensity	1200:1221	With increase in target DNA/miRNA concentration, the difference in fluorescence intensity also increased, resulting in a good linear relationship between fluorescence intensity sensitizing value and target miRNA concentrations.
32173991	7	14	theme	DNA	1599:1601	arg1	indicator					1610:1618	a DNA hybrid indicator	1597:1618	a DNA hybrid indicator	1597:1618	Therefore, a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free and no immobilization was established using lucigenin/chitosan/SiO2 composite nanoparticles as a DNA hybrid indicator.
32173991	7	14	theme	DNA	1599:1601	arg1	nanoparticles					1580:1592	lucigenin/chitosan/SiO2 composite nanoparticles	1546:1592	lucigenin/chitosan/SiO2 composite nanoparticles	1546:1592	Therefore, a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free and no immobilization was established using lucigenin/chitosan/SiO2 composite nanoparticles as a DNA hybrid indicator.
32173991	4	15	theme	lucigenin/chitosan/SiO2	919:941	arg1	nanoparticles					953:965	lucigenin/chitosan/SiO2 composite nanoparticles	919:965	lucigenin/chitosan/SiO2 composite nanoparticles with positive charge adsorbed using ssDNA or dsDNA	919:1016	Because the number of negative charges carried when using single-stranded DNA (ssDNA) was different from that of double-stranded DNA (dsDNA), the numbers of lucigenin/chitosan/SiO2 composite nanoparticles with positive charge adsorbed using ssDNA or dsDNA were different.
32173991	6	16	from	increase	1138:1145	arg1	concentration					1167:1179	target DNA/miRNA concentration	1150:1179	target DNA/miRNA concentration	1150:1179	With increase in target DNA/miRNA concentration, the difference in fluorescence intensity also increased, resulting in a good linear relationship between fluorescence intensity sensitizing value and target miRNA concentrations.
32173991	7	17	theme	analysis	1391:1398	arg1	method					1400:1405	a new fluorescence analysis method	1372:1405	a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free	1372:1500	Therefore, a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free and no immobilization was established using lucigenin/chitosan/SiO2 composite nanoparticles as a DNA hybrid indicator.
32173991	7	18	dep	detection	1418:1426	arg1	label					1491:1495	label	1491:1495	label free	1491:1500	Therefore, a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free and no immobilization was established using lucigenin/chitosan/SiO2 composite nanoparticles as a DNA hybrid indicator.
32173991	7	19	theme	hybrid	1603:1608	arg1	indicator					1610:1618	a DNA hybrid indicator	1597:1618	a DNA hybrid indicator	1597:1618	Therefore, a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free and no immobilization was established using lucigenin/chitosan/SiO2 composite nanoparticles as a DNA hybrid indicator.
32173991	7	19	theme	hybrid	1603:1608	arg1	nanoparticles					1580:1592	lucigenin/chitosan/SiO2 composite nanoparticles	1546:1592	lucigenin/chitosan/SiO2 composite nanoparticles	1546:1592	Therefore, a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free and no immobilization was established using lucigenin/chitosan/SiO2 composite nanoparticles as a DNA hybrid indicator.
32173991	5	20	theme	fluorescence	1048:1059	arg1	intensity					1061:1069	fluorescence intensity	1048:1069	fluorescence intensity caused using ssDNA or dsDNA/miRNA	1048:1103	Consequently, fluorescence intensity caused using ssDNA or dsDNA/miRNA was clearly discriminative.
32173991	6	21	theme	DNA/miRNA	1157:1165	arg1	concentration					1167:1179	target DNA/miRNA concentration	1150:1179	target DNA/miRNA concentration	1150:1179	With increase in target DNA/miRNA concentration, the difference in fluorescence intensity also increased, resulting in a good linear relationship between fluorescence intensity sensitizing value and target miRNA concentrations.
32173991	1	22	theme	microemulsion	188:200	arg1	method					202:207	a convenient reverse-phase microemulsion method	161:207	a convenient reverse-phase microemulsion method for the synthesis of SiO2 nanoparticles (NPs) by simply introducing the chitosan and fluorescent dye of lucigenin during the formation reaction of SiO2 NPs	161:363	In this paper, a convenient reverse-phase microemulsion method for the synthesis of SiO2 nanoparticles (NPs) by simply introducing the chitosan and fluorescent dye of lucigenin during the formation reaction of SiO2 NPs was proposed.
32173991	0	23	theme	magnetic	125:132	arg1	separation					134:143	magnetic separation	125:143	magnetic separation	125:143	A highly sensitive fluorescence sensor based on lucigenin/chitosan/SiO2 composite nanoparticles for microRNA detection using magnetic separation.
32173991	8	24	theme	low	1679:1681	arg1	cost					1683:1686	low cost	1679:1686	low cost	1679:1686	The proposed method had high sensitivity and selectivity, low cost and the detection limit was 10 fM (S/N = 3).
32173991	6	25	theme	linear	1259:1264	arg1	relationship					1266:1277	a good linear relationship	1252:1277	a good linear relationship between fluorescence intensity sensitizing value and target miRNA concentrations	1252:1358	With increase in target DNA/miRNA concentration, the difference in fluorescence intensity also increased, resulting in a good linear relationship between fluorescence intensity sensitizing value and target miRNA concentrations.
32173991	7	26	theme	cancer	1455:1460	arg1	samples					1467:1473	human gastric cancer cell samples	1441:1473	human gastric cancer cell samples	1441:1473	Therefore, a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free and no immobilization was established using lucigenin/chitosan/SiO2 composite nanoparticles as a DNA hybrid indicator.
32173991	2	27	theme	lucigenin	444:452	arg1	incorporation					463:475	lucigenin molecule incorporation	444:475	lucigenin molecule incorporation into chitosan/SiO2 NPs nanopores based on electrostatic interaction and supermolecular forces	444:569	Addition of chitosan can make the SiO2 NPs porous, and increases lucigenin molecule incorporation into chitosan/SiO2 NPs nanopores based on electrostatic interaction and supermolecular forces.
32173991	7	28	theme	direct	1411:1416	arg1	detection					1418:1426	direct detection	1411:1426	direct detection	1411:1426	Therefore, a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free and no immobilization was established using lucigenin/chitosan/SiO2 composite nanoparticles as a DNA hybrid indicator.
32173991	6	29	theme	good	1254:1257	arg1	relationship					1266:1277	a good linear relationship	1252:1277	a good linear relationship between fluorescence intensity sensitizing value and target miRNA concentrations	1252:1358	With increase in target DNA/miRNA concentration, the difference in fluorescence intensity also increased, resulting in a good linear relationship between fluorescence intensity sensitizing value and target miRNA concentrations.
32173991	2	30	theme	chitosan	391:398	arg1	Addition					379:386	Addition	379:386	Addition of chitosan	379:398	Addition of chitosan can make the SiO2 NPs porous, and increases lucigenin molecule incorporation into chitosan/SiO2 NPs nanopores based on electrostatic interaction and supermolecular forces.
32173991	6	31	theme	target	1150:1155	arg1	concentration					1167:1179	target DNA/miRNA concentration	1150:1179	target DNA/miRNA concentration	1150:1179	With increase in target DNA/miRNA concentration, the difference in fluorescence intensity also increased, resulting in a good linear relationship between fluorescence intensity sensitizing value and target miRNA concentrations.
32173991	0	32	theme	fluorescence	19:30	arg1	sensor					32:37	A highly sensitive fluorescence sensor	0:37	A highly sensitive fluorescence sensor based on lucigenin/chitosan/SiO2 composite	0:80	A highly sensitive fluorescence sensor based on lucigenin/chitosan/SiO2 composite nanoparticles for microRNA detection using magnetic separation.
32173991	1	33	theme	fluorescent	294:304	arg1	dye					306:308	fluorescent dye	294:308	fluorescent dye	294:308	In this paper, a convenient reverse-phase microemulsion method for the synthesis of SiO2 nanoparticles (NPs) by simply introducing the chitosan and fluorescent dye of lucigenin during the formation reaction of SiO2 NPs was proposed.
32173991	6	34	theme	target	1332:1337	arg1	concentrations					1345:1358	target miRNA concentrations	1332:1358	target miRNA concentrations	1332:1358	With increase in target DNA/miRNA concentration, the difference in fluorescence intensity also increased, resulting in a good linear relationship between fluorescence intensity sensitizing value and target miRNA concentrations.
32173991	3	35	theme	fluorescence	583:594	arg1	yield					604:608	fluorescence quantum yield	583:608	fluorescence quantum yield of the lucigenin/chitosan/SiO2 composite nanoparticles	583:663	Therefore, fluorescence quantum yield of the lucigenin/chitosan/SiO2 composite nanoparticles was increased by introduction of chitosan and compared with lucigenin/SiO2 NPs without chitosan.
32173991	4	36	theme	double-stranded	875:889	arg1	dsDNA					896:900	dsDNA	896:900	dsDNA	896:900	Because the number of negative charges carried when using single-stranded DNA (ssDNA) was different from that of double-stranded DNA (dsDNA), the numbers of lucigenin/chitosan/SiO2 composite nanoparticles with positive charge adsorbed using ssDNA or dsDNA were different.
32173991	4	36	theme	double-stranded	875:889	arg1	DNA					891:893	double-stranded DNA	875:893	double-stranded DNA (dsDNA)	875:901	Because the number of negative charges carried when using single-stranded DNA (ssDNA) was different from that of double-stranded DNA (dsDNA), the numbers of lucigenin/chitosan/SiO2 composite nanoparticles with positive charge adsorbed using ssDNA or dsDNA were different.
32173991	2	37	theme	porous	422:427	arg1	NPs					418:420	the SiO2 NPs	409:420	the SiO2 NPs porous	409:427	Addition of chitosan can make the SiO2 NPs porous, and increases lucigenin molecule incorporation into chitosan/SiO2 NPs nanopores based on electrostatic interaction and supermolecular forces.
32173991	0	38	theme	sensitive	9:17	arg1	sensor					32:37	A highly sensitive fluorescence sensor	0:37	A highly sensitive fluorescence sensor based on lucigenin/chitosan/SiO2 composite	0:80	A highly sensitive fluorescence sensor based on lucigenin/chitosan/SiO2 composite nanoparticles for microRNA detection using magnetic separation.
32173991	8	39	theme	detection	1696:1704	arg1	limit					1706:1710	the detection limit	1692:1710	the detection limit	1692:1710	The proposed method had high sensitivity and selectivity, low cost and the detection limit was 10 fM (S/N = 3).
32173991	8	39	theme	detection	1696:1704	arg1	fM					1719:1720	10 fM	1716:1720	10 fM (S/N = 3)	1716:1730	The proposed method had high sensitivity and selectivity, low cost and the detection limit was 10 fM (S/N = 3).
32173991	3	40	theme	quantum	596:602	arg1	yield					604:608	fluorescence quantum yield	583:608	fluorescence quantum yield of the lucigenin/chitosan/SiO2 composite nanoparticles	583:663	Therefore, fluorescence quantum yield of the lucigenin/chitosan/SiO2 composite nanoparticles was increased by introduction of chitosan and compared with lucigenin/SiO2 NPs without chitosan.
32173991	7	41	theme	let-7a	1431:1436	arg1	detection					1418:1426	direct detection	1411:1426	direct detection	1411:1426	Therefore, a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free and no immobilization was established using lucigenin/chitosan/SiO2 composite nanoparticles as a DNA hybrid indicator.
32173991	2	42	theme	chitosan/SiO2	482:494	arg1	nanopores					500:508	chitosan/SiO2 NPs nanopores	482:508	chitosan/SiO2 NPs nanopores based on electrostatic interaction and supermolecular forces	482:569	Addition of chitosan can make the SiO2 NPs porous, and increases lucigenin molecule incorporation into chitosan/SiO2 NPs nanopores based on electrostatic interaction and supermolecular forces.
32173991	1	43	theme	SiO2	230:233	arg1	NPs					250:252	NPs	250:252	NPs	250:252	In this paper, a convenient reverse-phase microemulsion method for the synthesis of SiO2 nanoparticles (NPs) by simply introducing the chitosan and fluorescent dye of lucigenin during the formation reaction of SiO2 NPs was proposed.
32173991	1	43	theme	SiO2	230:233	arg1	nanoparticles					235:247	SiO2 nanoparticles	230:247	SiO2 nanoparticles (NPs)	230:253	In this paper, a convenient reverse-phase microemulsion method for the synthesis of SiO2 nanoparticles (NPs) by simply introducing the chitosan and fluorescent dye of lucigenin during the formation reaction of SiO2 NPs was proposed.
32173991	3	44	theme	composite	641:649	arg1	nanoparticles					651:663	the lucigenin/chitosan/SiO2 composite nanoparticles	613:663	the lucigenin/chitosan/SiO2 composite nanoparticles	613:663	Therefore, fluorescence quantum yield of the lucigenin/chitosan/SiO2 composite nanoparticles was increased by introduction of chitosan and compared with lucigenin/SiO2 NPs without chitosan.
32173991	4	45	with	nanoparticles	953:965	arg1	charge					981:986	positive charge	972:986	positive charge adsorbed using ssDNA or dsDNA	972:1016	Because the number of negative charges carried when using single-stranded DNA (ssDNA) was different from that of double-stranded DNA (dsDNA), the numbers of lucigenin/chitosan/SiO2 composite nanoparticles with positive charge adsorbed using ssDNA or dsDNA were different.
32173991	2	46	theme	SiO2	413:416	arg1	NPs					418:420	the SiO2 NPs	409:420	the SiO2 NPs porous	409:427	Addition of chitosan can make the SiO2 NPs porous, and increases lucigenin molecule incorporation into chitosan/SiO2 NPs nanopores based on electrostatic interaction and supermolecular forces.
32173991	7	47	theme	gastric	1447:1453	arg1	samples					1467:1473	human gastric cancer cell samples	1441:1473	human gastric cancer cell samples	1441:1473	Therefore, a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free and no immobilization was established using lucigenin/chitosan/SiO2 composite nanoparticles as a DNA hybrid indicator.
32173991	1	48	theme	lucigenin	313:321	arg1	dye					306:308	fluorescent dye	294:308	fluorescent dye	294:308	In this paper, a convenient reverse-phase microemulsion method for the synthesis of SiO2 nanoparticles (NPs) by simply introducing the chitosan and fluorescent dye of lucigenin during the formation reaction of SiO2 NPs was proposed.
32173991	1	48	theme	lucigenin	313:321	arg1	chitosan					281:288	chitosan	281:288	chitosan	281:288	In this paper, a convenient reverse-phase microemulsion method for the synthesis of SiO2 nanoparticles (NPs) by simply introducing the chitosan and fluorescent dye of lucigenin during the formation reaction of SiO2 NPs was proposed.
32173991	3	49	theme	nanoparticles	651:663	arg1	yield					604:608	fluorescence quantum yield	583:608	fluorescence quantum yield of the lucigenin/chitosan/SiO2 composite nanoparticles	583:663	Therefore, fluorescence quantum yield of the lucigenin/chitosan/SiO2 composite nanoparticles was increased by introduction of chitosan and compared with lucigenin/SiO2 NPs without chitosan.
32173991	8	50	contain	had	1641:1643	arg2	cost					1683:1686	low cost	1679:1686	low cost	1679:1686	The proposed method had high sensitivity and selectivity, low cost and the detection limit was 10 fM (S/N = 3).
32173991	8	50	contain	had	1641:1643	arg1	method					1634:1639	The proposed method	1621:1639	The proposed method	1621:1639	The proposed method had high sensitivity and selectivity, low cost and the detection limit was 10 fM (S/N = 3).
32173991	8	50	contain	had	1641:1643	arg2	sensitivity					1650:1660	high sensitivity	1645:1660	high sensitivity	1645:1660	The proposed method had high sensitivity and selectivity, low cost and the detection limit was 10 fM (S/N = 3).
32173991	8	50	contain	had	1641:1643	arg2	selectivity					1666:1676	selectivity	1666:1676	selectivity	1666:1676	The proposed method had high sensitivity and selectivity, low cost and the detection limit was 10 fM (S/N = 3).
32173991	1	51	theme	nanoparticles	235:247	arg1	synthesis					217:225	the synthesis	213:225	the synthesis of SiO2 nanoparticles (NPs) by simply introducing the chitosan and fluorescent dye of lucigenin during the formation reaction of SiO2 NPs	213:363	In this paper, a convenient reverse-phase microemulsion method for the synthesis of SiO2 nanoparticles (NPs) by simply introducing the chitosan and fluorescent dye of lucigenin during the formation reaction of SiO2 NPs was proposed.
32173991	0	52	theme	lucigenin/chitosan/SiO2	48:70	arg1	composite					72:80	lucigenin/chitosan/SiO2 composite	48:80	lucigenin/chitosan/SiO2 composite	48:80	A highly sensitive fluorescence sensor based on lucigenin/chitosan/SiO2 composite nanoparticles for microRNA detection using magnetic separation.
32173991	3	53	theme	chitosan	698:705	arg1	introduction					682:693	introduction	682:693	introduction of chitosan	682:705	Therefore, fluorescence quantum yield of the lucigenin/chitosan/SiO2 composite nanoparticles was increased by introduction of chitosan and compared with lucigenin/SiO2 NPs without chitosan.
32173991	6	54	theme	miRNA	1339:1343	arg1	concentrations					1345:1358	target miRNA concentrations	1332:1358	target miRNA concentrations	1332:1358	With increase in target DNA/miRNA concentration, the difference in fluorescence intensity also increased, resulting in a good linear relationship between fluorescence intensity sensitizing value and target miRNA concentrations.
32173991	7	55	from	detection	1418:1426	arg1	samples					1467:1473	human gastric cancer cell samples	1441:1473	human gastric cancer cell samples	1441:1473	Therefore, a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free and no immobilization was established using lucigenin/chitosan/SiO2 composite nanoparticles as a DNA hybrid indicator.
32173991	2	56	theme	molecule	454:461	arg1	incorporation					463:475	lucigenin molecule incorporation	444:475	lucigenin molecule incorporation into chitosan/SiO2 NPs nanopores based on electrostatic interaction and supermolecular forces	444:569	Addition of chitosan can make the SiO2 NPs porous, and increases lucigenin molecule incorporation into chitosan/SiO2 NPs nanopores based on electrostatic interaction and supermolecular forces.
32173991	4	57	theme	nanoparticles	953:965	arg1	different					1023:1031	different	1023:1031	different	1023:1031	Because the number of negative charges carried when using single-stranded DNA (ssDNA) was different from that of double-stranded DNA (dsDNA), the numbers of lucigenin/chitosan/SiO2 composite nanoparticles with positive charge adsorbed using ssDNA or dsDNA were different.
32173991	4	57	theme	nanoparticles	953:965	arg1	numbers					908:914	the numbers	904:914	the numbers of lucigenin/chitosan/SiO2 composite nanoparticles with positive charge adsorbed using ssDNA or dsDNA	904:1016	Because the number of negative charges carried when using single-stranded DNA (ssDNA) was different from that of double-stranded DNA (dsDNA), the numbers of lucigenin/chitosan/SiO2 composite nanoparticles with positive charge adsorbed using ssDNA or dsDNA were different.
32173991	3	58	theme	lucigenin/chitosan/SiO2	617:639	arg1	nanoparticles					651:663	the lucigenin/chitosan/SiO2 composite nanoparticles	613:663	the lucigenin/chitosan/SiO2 composite nanoparticles	613:663	Therefore, fluorescence quantum yield of the lucigenin/chitosan/SiO2 composite nanoparticles was increased by introduction of chitosan and compared with lucigenin/SiO2 NPs without chitosan.
32173991	1	59	theme	formation	334:342	arg1	reaction					344:351	the formation reaction	330:351	the formation reaction of SiO2 NPs	330:363	In this paper, a convenient reverse-phase microemulsion method for the synthesis of SiO2 nanoparticles (NPs) by simply introducing the chitosan and fluorescent dye of lucigenin during the formation reaction of SiO2 NPs was proposed.
32173991	1	60	dep	chitosan	281:288	arg1	the					277:279	the	277:279	the	277:279	In this paper, a convenient reverse-phase microemulsion method for the synthesis of SiO2 nanoparticles (NPs) by simply introducing the chitosan and fluorescent dye of lucigenin during the formation reaction of SiO2 NPs was proposed.
32173991	4	61	theme	charges	793:799	arg1	different					852:860	different	852:860	different	852:860	Because the number of negative charges carried when using single-stranded DNA (ssDNA) was different from that of double-stranded DNA (dsDNA), the numbers of lucigenin/chitosan/SiO2 composite nanoparticles with positive charge adsorbed using ssDNA or dsDNA were different.
32173991	4	61	theme	charges	793:799	arg1	number					774:779	the number	770:779	the number of negative charges carried when using single-stranded DNA (ssDNA)	770:846	Because the number of negative charges carried when using single-stranded DNA (ssDNA) was different from that of double-stranded DNA (dsDNA), the numbers of lucigenin/chitosan/SiO2 composite nanoparticles with positive charge adsorbed using ssDNA or dsDNA were different.
32173991	4	62	theme	single-stranded	820:834	arg1	ssDNA					841:845	ssDNA	841:845	ssDNA	841:845	Because the number of negative charges carried when using single-stranded DNA (ssDNA) was different from that of double-stranded DNA (dsDNA), the numbers of lucigenin/chitosan/SiO2 composite nanoparticles with positive charge adsorbed using ssDNA or dsDNA were different.
32173991	4	62	theme	single-stranded	820:834	arg1	DNA					836:838	single-stranded DNA	820:838	single-stranded DNA (ssDNA)	820:846	Because the number of negative charges carried when using single-stranded DNA (ssDNA) was different from that of double-stranded DNA (dsDNA), the numbers of lucigenin/chitosan/SiO2 composite nanoparticles with positive charge adsorbed using ssDNA or dsDNA were different.
32173991	4	63	theme	positive	972:979	arg1	charge					981:986	positive charge	972:986	positive charge adsorbed using ssDNA or dsDNA	972:1016	Because the number of negative charges carried when using single-stranded DNA (ssDNA) was different from that of double-stranded DNA (dsDNA), the numbers of lucigenin/chitosan/SiO2 composite nanoparticles with positive charge adsorbed using ssDNA or dsDNA were different.
32173991	8	64	theme	proposed	1625:1632	arg1	method					1634:1639	The proposed method	1621:1639	The proposed method	1621:1639	The proposed method had high sensitivity and selectivity, low cost and the detection limit was 10 fM (S/N = 3).
32173991	7	65	theme	composite	1570:1578	arg1	indicator					1610:1618	a DNA hybrid indicator	1597:1618	a DNA hybrid indicator	1597:1618	Therefore, a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free and no immobilization was established using lucigenin/chitosan/SiO2 composite nanoparticles as a DNA hybrid indicator.
32173991	7	65	theme	composite	1570:1578	arg1	nanoparticles					1580:1592	lucigenin/chitosan/SiO2 composite nanoparticles	1546:1592	lucigenin/chitosan/SiO2 composite nanoparticles	1546:1592	Therefore, a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free and no immobilization was established using lucigenin/chitosan/SiO2 composite nanoparticles as a DNA hybrid indicator.
32173991	4	66	from	that	867:870	arg1	different					852:860	different	852:860	different	852:860	Because the number of negative charges carried when using single-stranded DNA (ssDNA) was different from that of double-stranded DNA (dsDNA), the numbers of lucigenin/chitosan/SiO2 composite nanoparticles with positive charge adsorbed using ssDNA or dsDNA were different.
32173991	4	66	from	that	867:870	arg1	number					774:779	the number	770:779	the number of negative charges carried when using single-stranded DNA (ssDNA)	770:846	Because the number of negative charges carried when using single-stranded DNA (ssDNA) was different from that of double-stranded DNA (dsDNA), the numbers of lucigenin/chitosan/SiO2 composite nanoparticles with positive charge adsorbed using ssDNA or dsDNA were different.
32173991	4	67	theme	negative	784:791	arg1	charges					793:799	negative charges	784:799	negative charges carried when using single-stranded DNA (ssDNA)	784:846	Because the number of negative charges carried when using single-stranded DNA (ssDNA) was different from that of double-stranded DNA (dsDNA), the numbers of lucigenin/chitosan/SiO2 composite nanoparticles with positive charge adsorbed using ssDNA or dsDNA were different.
32173991	7	68	theme	fluorescence	1378:1389	arg1	method					1400:1405	a new fluorescence analysis method	1372:1405	a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free	1372:1500	Therefore, a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free and no immobilization was established using lucigenin/chitosan/SiO2 composite nanoparticles as a DNA hybrid indicator.
32173991	3	69	theme	lucigenin/SiO2	725:738	arg1	NPs					740:742	lucigenin/SiO2 NPs	725:742	lucigenin/SiO2 NPs without chitosan	725:759	Therefore, fluorescence quantum yield of the lucigenin/chitosan/SiO2 composite nanoparticles was increased by introduction of chitosan and compared with lucigenin/SiO2 NPs without chitosan.
32173991	7	70	theme	cell	1462:1465	arg1	samples					1467:1473	human gastric cancer cell samples	1441:1473	human gastric cancer cell samples	1441:1473	Therefore, a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free and no immobilization was established using lucigenin/chitosan/SiO2 composite nanoparticles as a DNA hybrid indicator.
32173991	6	71	theme	fluorescence	1200:1211	arg1	intensity					1213:1221	fluorescence intensity	1200:1221	fluorescence intensity	1200:1221	With increase in target DNA/miRNA concentration, the difference in fluorescence intensity also increased, resulting in a good linear relationship between fluorescence intensity sensitizing value and target miRNA concentrations.
32173991	7	72	theme	new	1374:1376	arg1	method					1400:1405	a new fluorescence analysis method	1372:1405	a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free	1372:1500	Therefore, a new fluorescence analysis method for direct detection of let-7a in human gastric cancer cell samples without enzyme, label free and no immobilization was established using lucigenin/chitosan/SiO2 composite nanoparticles as a DNA hybrid indicator.
32173991	8	73	theme	=	1727:1727	arg1	S/N					1723:1725	S/N = 3	1723:1729	S/N = 3	1723:1729	The proposed method had high sensitivity and selectivity, low cost and the detection limit was 10 fM (S/N = 3).
32173991	8	73	theme	=	1727:1727	arg1	fM					1719:1720	10 fM	1716:1720	10 fM (S/N = 3)	1716:1730	The proposed method had high sensitivity and selectivity, low cost and the detection limit was 10 fM (S/N = 3).
33516130	7	0	theme	promising	1440:1448	arg1	results					1450:1456	promising results	1440:1456	promising results for the intended application	1440:1485	The results showed promising results for the intended application.
33516130	3	1	theme	calcium	720:726	arg1	phosphate					728:736	calcium phosphate	720:736	calcium phosphate	720:736	In this work, chitosan was blended with 10% glycerol and 10% glycerol + 10% biphasic mixture of calcium phosphate (70% hydroxyapatite with 30% β-tricalcium phosphate) to produce dense chitosan-based blocks, which were then shaped into rods.
33516130	6	2	theme	strain	1261:1266	arg1	conditions					1273:1282	medium strain rate conditions	1254:1282	medium strain rate conditions (200-800 s-1)	1254:1296	The specimens were analysed in quasi-static conditions (less than 0.1 s-1) and medium strain rate conditions (200-800 s-1), both in dry state and in different hydrated conditions, in the latter case to approximate the in vivo implant conditions.
33516130	6	2	theme	strain	1261:1266	arg1	s-1					1293:1295	200-800 s-1	1285:1295	200-800 s-1	1285:1295	The specimens were analysed in quasi-static conditions (less than 0.1 s-1) and medium strain rate conditions (200-800 s-1), both in dry state and in different hydrated conditions, in the latter case to approximate the in vivo implant conditions.
33516130	3	3	dep	glycerol	685:692	arg1	%					741:741	70%	739:741	70% hydroxyapatite with 30% β-tricalcium phosphate	739:788	In this work, chitosan was blended with 10% glycerol and 10% glycerol + 10% biphasic mixture of calcium phosphate (70% hydroxyapatite with 30% β-tricalcium phosphate) to produce dense chitosan-based blocks, which were then shaped into rods.
33516130	2	4	dep	producing	585:593	arg1	structures					612:621	such structures	607:621	such structures	607:621	Porous chitosan scaffolds have been extensively studied and explored in bone generation, however it is still a challenge to obtain bioabsorbable orthopedic implants that involves pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures.
33516130	9	5	theme	behavior	1863:1870	arg1	modelling					1836:1844	future modelling	1829:1844	future modelling of this material behavior	1829:1870	An empirical model was proposed to describe the flow stress curves, with a good agreement with the experimental data, allowing future modelling of this material behavior.
33516130	8	6	from	behavior	1568:1575	arg1	conditions					1593:1602	quasi-static conditions	1580:1602	quasi-static conditions	1580:1602	The chitosan blends present excellent ductility with an elastic perfect-plastic behavior in quasi-static conditions, with yield stresses around 40 MPa for the dry state, with a decay for 3 MPa after 48h hydration.
33516130	9	7	with	agreement	1782:1790	arg1	data					1814:1817	the experimental data	1797:1817	the experimental data	1797:1817	An empirical model was proposed to describe the flow stress curves, with a good agreement with the experimental data, allowing future modelling of this material behavior.
33516130	8	8	theme	48h	1687:1689	arg1	hydration					1691:1699	48h hydration	1687:1699	48h hydration	1687:1699	The chitosan blends present excellent ductility with an elastic perfect-plastic behavior in quasi-static conditions, with yield stresses around 40 MPa for the dry state, with a decay for 3 MPa after 48h hydration.
33516130	2	9	theme	Porous	336:341	arg1	scaffolds					352:360	Porous chitosan scaffolds	336:360	Porous chitosan scaffolds	336:360	Porous chitosan scaffolds have been extensively studied and explored in bone generation, however it is still a challenge to obtain bioabsorbable orthopedic implants that involves pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures.
33516130	1	10	theme	scientific	191:200	arg1	community					202:210	the scientific community	187:210	the scientific community	187:210	Chitosan has generated enormous interest in the scientific community because of its distinctive biological and physicochemical properties, which allow new advanced structures and applications.
33516130	6	11	theme	dry	1307:1309	arg1	state					1311:1315	dry state	1307:1315	dry state	1307:1315	The specimens were analysed in quasi-static conditions (less than 0.1 s-1) and medium strain rate conditions (200-800 s-1), both in dry state and in different hydrated conditions, in the latter case to approximate the in vivo implant conditions.
33516130	4	12	used	used	971:974	arg2	particles					956:964	the ceramic particles	944:964	the ceramic particles	944:964	The introduction of plasticizer aimed to improve the materials ductility while the ceramic particles were used to increase stiffness and strength.
33516130	4	13	theme	materials	918:926	arg1	ductility					928:936	the materials ductility	914:936	the materials ductility	914:936	The introduction of plasticizer aimed to improve the materials ductility while the ceramic particles were used to increase stiffness and strength.
33516130	3	14	theme	%	765:765	arg1	phosphate					780:788	30% β-tricalcium phosphate	763:788	30% β-tricalcium phosphate	763:788	In this work, chitosan was blended with 10% glycerol and 10% glycerol + 10% biphasic mixture of calcium phosphate (70% hydroxyapatite with 30% β-tricalcium phosphate) to produce dense chitosan-based blocks, which were then shaped into rods.
33516130	6	15	theme	in	1393:1394	arg1	conditions					1409:1418	the in vivo implant conditions	1389:1418	the in vivo implant conditions	1389:1418	The specimens were analysed in quasi-static conditions (less than 0.1 s-1) and medium strain rate conditions (200-800 s-1), both in dry state and in different hydrated conditions, in the latter case to approximate the in vivo implant conditions.
33516130	2	16	theme	chitosan	529:536	arg1	geometries					538:547	pure 3D dense chitosan geometries	515:547	pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures	515:621	Porous chitosan scaffolds have been extensively studied and explored in bone generation, however it is still a challenge to obtain bioabsorbable orthopedic implants that involves pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures.
33516130	1	17	from	interest	175:182	arg1	community					202:210	the scientific community	187:210	the scientific community	187:210	Chitosan has generated enormous interest in the scientific community because of its distinctive biological and physicochemical properties, which allow new advanced structures and applications.
33516130	2	18	theme	3D	520:521	arg1	geometries					538:547	pure 3D dense chitosan geometries	515:547	pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures	515:621	Porous chitosan scaffolds have been extensively studied and explored in bone generation, however it is still a challenge to obtain bioabsorbable orthopedic implants that involves pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures.
33516130	9	19	theme	empirical	1705:1713	arg1	model					1715:1719	An empirical model	1702:1719	An empirical model	1702:1719	An empirical model was proposed to describe the flow stress curves, with a good agreement with the experimental data, allowing future modelling of this material behavior.
33516130	5	20	theme	split-Hopkinson	1138:1152	arg1	SHPB					1168:1171	SHPB	1168:1171	SHPB	1168:1171	The mechanical behavior of the two chitosan-based compositions was evaluated by uniaxial compression tests using a customized split-Hopkinson pressure bar (SHPB).
33516130	5	20	theme	split-Hopkinson	1138:1152	arg1	bar					1163:1165	a customized split-Hopkinson pressure bar	1125:1165	a customized split-Hopkinson pressure bar (SHPB)	1125:1172	The mechanical behavior of the two chitosan-based compositions was evaluated by uniaxial compression tests using a customized split-Hopkinson pressure bar (SHPB).
33516130	1	21	theme	enormous	166:173	arg1	interest					175:182	enormous interest	166:182	enormous interest in the scientific community	166:210	Chitosan has generated enormous interest in the scientific community because of its distinctive biological and physicochemical properties, which allow new advanced structures and applications.
33516130	0	22	from	Influence	0:8	arg1	behavior					43:50	the mechanical behavior	28:50	the mechanical behavior of dry and hydrated chitosan-based dense materials for bioabsorbable implant applications	28:140	Influence of strain rate on the mechanical behavior of dry and hydrated chitosan-based dense materials for bioabsorbable implant applications.
33516130	3	23	theme	dense	802:806	arg1	blocks					823:828	dense chitosan-based blocks	802:828	dense chitosan-based blocks	802:828	In this work, chitosan was blended with 10% glycerol and 10% glycerol + 10% biphasic mixture of calcium phosphate (70% hydroxyapatite with 30% β-tricalcium phosphate) to produce dense chitosan-based blocks, which were then shaped into rods.
33516130	0	24	theme	chitosan-based	72:85	arg1	materials					93:101	dry and hydrated chitosan-based dense materials	55:101	dry and hydrated chitosan-based dense materials	55:101	Influence of strain rate on the mechanical behavior of dry and hydrated chitosan-based dense materials for bioabsorbable implant applications.
33516130	5	25	theme	uniaxial	1092:1099	arg1	tests					1113:1117	uniaxial compression tests	1092:1117	uniaxial compression tests using a customized split-Hopkinson pressure bar (SHPB)	1092:1172	The mechanical behavior of the two chitosan-based compositions was evaluated by uniaxial compression tests using a customized split-Hopkinson pressure bar (SHPB).
33516130	2	26	theme	due	549:551	arg1	geometries					538:547	pure 3D dense chitosan geometries	515:547	pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures	515:621	Porous chitosan scaffolds have been extensively studied and explored in bone generation, however it is still a challenge to obtain bioabsorbable orthopedic implants that involves pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures.
33516130	0	27	theme	materials	93:101	arg1	behavior					43:50	the mechanical behavior	28:50	the mechanical behavior of dry and hydrated chitosan-based dense materials for bioabsorbable implant applications	28:140	Influence of strain rate on the mechanical behavior of dry and hydrated chitosan-based dense materials for bioabsorbable implant applications.
33516130	2	28	theme	bioabsorbable	467:479	arg1	implants					492:499	bioabsorbable orthopedic implants	467:499	bioabsorbable orthopedic implants that involves pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures	467:621	Porous chitosan scaffolds have been extensively studied and explored in bone generation, however it is still a challenge to obtain bioabsorbable orthopedic implants that involves pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures.
33516130	1	29	theme	new	294:296	arg1	structures					307:316	new advanced structures	294:316	new advanced structures	294:316	Chitosan has generated enormous interest in the scientific community because of its distinctive biological and physicochemical properties, which allow new advanced structures and applications.
33516130	8	30	theme	excellent	1516:1524	arg1	ductility					1526:1534	excellent ductility	1516:1534	excellent ductility with an elastic perfect-plastic behavior in quasi-static conditions	1516:1602	The chitosan blends present excellent ductility with an elastic perfect-plastic behavior in quasi-static conditions, with yield stresses around 40 MPa for the dry state, with a decay for 3 MPa after 48h hydration.
33516130	0	31	theme	strain	13:18	arg1	rate					20:23	strain rate	13:23	strain rate	13:23	Influence of strain rate on the mechanical behavior of dry and hydrated chitosan-based dense materials for bioabsorbable implant applications.
33516130	9	32	theme	good	1777:1780	arg1	agreement					1782:1790	a good agreement	1775:1790	a good agreement with the experimental data	1775:1817	An empirical model was proposed to describe the flow stress curves, with a good agreement with the experimental data, allowing future modelling of this material behavior.
33516130	8	33	theme	elastic	1544:1550	arg1	behavior					1568:1575	an elastic perfect-plastic behavior	1541:1575	an elastic perfect-plastic behavior in quasi-static conditions	1541:1602	The chitosan blends present excellent ductility with an elastic perfect-plastic behavior in quasi-static conditions, with yield stresses around 40 MPa for the dry state, with a decay for 3 MPa after 48h hydration.
33516130	3	34	theme	10	664:665	arg1	%					666:666	%	666:666	%	666:666	In this work, chitosan was blended with 10% glycerol and 10% glycerol + 10% biphasic mixture of calcium phosphate (70% hydroxyapatite with 30% β-tricalcium phosphate) to produce dense chitosan-based blocks, which were then shaped into rods.
33516130	0	35	theme	mechanical	32:41	arg1	behavior					43:50	the mechanical behavior	28:50	the mechanical behavior of dry and hydrated chitosan-based dense materials for bioabsorbable implant applications	28:140	Influence of strain rate on the mechanical behavior of dry and hydrated chitosan-based dense materials for bioabsorbable implant applications.
33516130	4	36	theme	ceramic	948:954	arg1	particles					956:964	the ceramic particles	944:964	the ceramic particles	944:964	The introduction of plasticizer aimed to improve the materials ductility while the ceramic particles were used to increase stiffness and strength.
33516130	1	37	theme	distinctive	227:237	arg1	properties					270:279	its distinctive biological and physicochemical properties	223:279	its distinctive biological and physicochemical properties	223:279	Chitosan has generated enormous interest in the scientific community because of its distinctive biological and physicochemical properties, which allow new advanced structures and applications.
33516130	8	38	theme	quasi-static	1580:1591	arg1	conditions					1593:1602	quasi-static conditions	1580:1602	quasi-static conditions	1580:1602	The chitosan blends present excellent ductility with an elastic perfect-plastic behavior in quasi-static conditions, with yield stresses around 40 MPa for the dry state, with a decay for 3 MPa after 48h hydration.
33516130	3	39	theme	10	681:682	arg1	%					683:683	%	683:683	%	683:683	In this work, chitosan was blended with 10% glycerol and 10% glycerol + 10% biphasic mixture of calcium phosphate (70% hydroxyapatite with 30% β-tricalcium phosphate) to produce dense chitosan-based blocks, which were then shaped into rods.
33516130	8	40	theme	dry	1647:1649	arg1	state					1651:1655	the dry state	1643:1655	the dry state	1643:1655	The chitosan blends present excellent ductility with an elastic perfect-plastic behavior in quasi-static conditions, with yield stresses around 40 MPa for the dry state, with a decay for 3 MPa after 48h hydration.
33516130	9	41	theme	future	1829:1834	arg1	modelling					1836:1844	future modelling	1829:1844	future modelling of this material behavior	1829:1870	An empirical model was proposed to describe the flow stress curves, with a good agreement with the experimental data, allowing future modelling of this material behavior.
33516130	3	42	theme	biphasic	700:707	arg1	mixture					709:715	10% biphasic mixture	696:715	10% biphasic mixture	696:715	In this work, chitosan was blended with 10% glycerol and 10% glycerol + 10% biphasic mixture of calcium phosphate (70% hydroxyapatite with 30% β-tricalcium phosphate) to produce dense chitosan-based blocks, which were then shaped into rods.
33516130	8	43	theme	yield	1610:1614	arg1	stresses					1616:1623	yield stresses	1610:1623	yield stresses around 40 MPa for the dry state	1610:1655	The chitosan blends present excellent ductility with an elastic perfect-plastic behavior in quasi-static conditions, with yield stresses around 40 MPa for the dry state, with a decay for 3 MPa after 48h hydration.
33516130	3	44	theme	10	696:697	arg1	%					698:698	%	698:698	%	698:698	In this work, chitosan was blended with 10% glycerol and 10% glycerol + 10% biphasic mixture of calcium phosphate (70% hydroxyapatite with 30% β-tricalcium phosphate) to produce dense chitosan-based blocks, which were then shaped into rods.
33516130	6	45	theme	rate	1268:1271	arg1	conditions					1273:1282	medium strain rate conditions	1254:1282	medium strain rate conditions (200-800 s-1)	1254:1296	The specimens were analysed in quasi-static conditions (less than 0.1 s-1) and medium strain rate conditions (200-800 s-1), both in dry state and in different hydrated conditions, in the latter case to approximate the in vivo implant conditions.
33516130	6	45	theme	rate	1268:1271	arg1	s-1					1293:1295	200-800 s-1	1285:1295	200-800 s-1	1285:1295	The specimens were analysed in quasi-static conditions (less than 0.1 s-1) and medium strain rate conditions (200-800 s-1), both in dry state and in different hydrated conditions, in the latter case to approximate the in vivo implant conditions.
33516130	9	46	theme	material	1854:1861	arg1	behavior					1863:1870	this material behavior	1849:1870	this material behavior	1849:1870	An empirical model was proposed to describe the flow stress curves, with a good agreement with the experimental data, allowing future modelling of this material behavior.
33516130	3	47	theme	phosphate	728:736	arg1	glycerol					668:675	10% glycerol	664:675	10% glycerol	664:675	In this work, chitosan was blended with 10% glycerol and 10% glycerol + 10% biphasic mixture of calcium phosphate (70% hydroxyapatite with 30% β-tricalcium phosphate) to produce dense chitosan-based blocks, which were then shaped into rods.
33516130	3	47	theme	phosphate	728:736	arg1	mixture					709:715	10% biphasic mixture	696:715	10% biphasic mixture	696:715	In this work, chitosan was blended with 10% glycerol and 10% glycerol + 10% biphasic mixture of calcium phosphate (70% hydroxyapatite with 30% β-tricalcium phosphate) to produce dense chitosan-based blocks, which were then shaped into rods.
33516130	3	47	theme	phosphate	728:736	arg1	glycerol					685:692	10% glycerol	681:692	10% glycerol	681:692	In this work, chitosan was blended with 10% glycerol and 10% glycerol + 10% biphasic mixture of calcium phosphate (70% hydroxyapatite with 30% β-tricalcium phosphate) to produce dense chitosan-based blocks, which were then shaped into rods.
33516130	6	48	theme	medium	1254:1259	arg1	conditions					1273:1282	medium strain rate conditions	1254:1282	medium strain rate conditions (200-800 s-1)	1254:1296	The specimens were analysed in quasi-static conditions (less than 0.1 s-1) and medium strain rate conditions (200-800 s-1), both in dry state and in different hydrated conditions, in the latter case to approximate the in vivo implant conditions.
33516130	6	48	theme	medium	1254:1259	arg1	s-1					1293:1295	200-800 s-1	1285:1295	200-800 s-1	1285:1295	The specimens were analysed in quasi-static conditions (less than 0.1 s-1) and medium strain rate conditions (200-800 s-1), both in dry state and in different hydrated conditions, in the latter case to approximate the in vivo implant conditions.
33516130	6	49	theme	latter	1362:1367	arg1	case					1369:1372	the latter case	1358:1372	the latter case to approximate the in vivo implant conditions	1358:1418	The specimens were analysed in quasi-static conditions (less than 0.1 s-1) and medium strain rate conditions (200-800 s-1), both in dry state and in different hydrated conditions, in the latter case to approximate the in vivo implant conditions.
33516130	2	50	theme	chitosan	343:350	arg1	scaffolds					352:360	Porous chitosan scaffolds	336:360	Porous chitosan scaffolds	336:360	Porous chitosan scaffolds have been extensively studied and explored in bone generation, however it is still a challenge to obtain bioabsorbable orthopedic implants that involves pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures.
33516130	3	51	theme	hydroxyapatite	743:756	arg1	%					741:741	70%	739:741	70% hydroxyapatite with 30% β-tricalcium phosphate	739:788	In this work, chitosan was blended with 10% glycerol and 10% glycerol + 10% biphasic mixture of calcium phosphate (70% hydroxyapatite with 30% β-tricalcium phosphate) to produce dense chitosan-based blocks, which were then shaped into rods.
33516130	0	52	theme	implant	121:127	arg1	applications					129:140	bioabsorbable implant applications	107:140	bioabsorbable implant applications	107:140	Influence of strain rate on the mechanical behavior of dry and hydrated chitosan-based dense materials for bioabsorbable implant applications.
33516130	9	53	theme	experimental	1801:1812	arg1	data					1814:1817	the experimental data	1797:1817	the experimental data	1797:1817	An empirical model was proposed to describe the flow stress curves, with a good agreement with the experimental data, allowing future modelling of this material behavior.
33516130	2	54	theme	inherent	560:567	arg1	difficulties					569:580	the inherent difficulties	556:580	the inherent difficulties in producing and shaping such structures	556:621	Porous chitosan scaffolds have been extensively studied and explored in bone generation, however it is still a challenge to obtain bioabsorbable orthopedic implants that involves pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures.
33516130	3	55	theme	β-tricalcium	767:778	arg1	phosphate					780:788	30% β-tricalcium phosphate	763:788	30% β-tricalcium phosphate	763:788	In this work, chitosan was blended with 10% glycerol and 10% glycerol + 10% biphasic mixture of calcium phosphate (70% hydroxyapatite with 30% β-tricalcium phosphate) to produce dense chitosan-based blocks, which were then shaped into rods.
33516130	6	56	theme	hydrated	1334:1341	arg1	conditions					1343:1352	different hydrated conditions	1324:1352	different hydrated conditions	1324:1352	The specimens were analysed in quasi-static conditions (less than 0.1 s-1) and medium strain rate conditions (200-800 s-1), both in dry state and in different hydrated conditions, in the latter case to approximate the in vivo implant conditions.
33516130	8	57	with	ductility	1526:1534	arg1	behavior					1568:1575	an elastic perfect-plastic behavior	1541:1575	an elastic perfect-plastic behavior in quasi-static conditions	1541:1602	The chitosan blends present excellent ductility with an elastic perfect-plastic behavior in quasi-static conditions, with yield stresses around 40 MPa for the dry state, with a decay for 3 MPa after 48h hydration.
33516130	2	58	theme	such	607:610	arg1	structures					612:621	such structures	607:621	such structures	607:621	Porous chitosan scaffolds have been extensively studied and explored in bone generation, however it is still a challenge to obtain bioabsorbable orthopedic implants that involves pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures.
33516130	3	59	theme	30	763:764	arg1	%					765:765	%	765:765	%	765:765	In this work, chitosan was blended with 10% glycerol and 10% glycerol + 10% biphasic mixture of calcium phosphate (70% hydroxyapatite with 30% β-tricalcium phosphate) to produce dense chitosan-based blocks, which were then shaped into rods.
33516130	4	60	theme	plasticizer	885:895	arg1	introduction					869:880	The introduction	865:880	The introduction of plasticizer	865:895	The introduction of plasticizer aimed to improve the materials ductility while the ceramic particles were used to increase stiffness and strength.
33516130	6	61	theme	quasi-static	1206:1217	arg1	s-1					1245:1247	less than 0.1 s-1	1231:1247	less than 0.1 s-1	1231:1247	The specimens were analysed in quasi-static conditions (less than 0.1 s-1) and medium strain rate conditions (200-800 s-1), both in dry state and in different hydrated conditions, in the latter case to approximate the in vivo implant conditions.
33516130	6	61	theme	quasi-static	1206:1217	arg1	conditions					1219:1228	quasi-static conditions	1206:1228	quasi-static conditions (less than 0.1 s-1)	1206:1248	The specimens were analysed in quasi-static conditions (less than 0.1 s-1) and medium strain rate conditions (200-800 s-1), both in dry state and in different hydrated conditions, in the latter case to approximate the in vivo implant conditions.
33516130	0	62	theme	dry	55:57	arg1	materials					93:101	dry and hydrated chitosan-based dense materials	55:101	dry and hydrated chitosan-based dense materials	55:101	Influence of strain rate on the mechanical behavior of dry and hydrated chitosan-based dense materials for bioabsorbable implant applications.
33516130	5	63	theme	chitosan-based	1047:1060	arg1	compositions					1062:1073	the two chitosan-based compositions	1039:1073	the two chitosan-based compositions	1039:1073	The mechanical behavior of the two chitosan-based compositions was evaluated by uniaxial compression tests using a customized split-Hopkinson pressure bar (SHPB).
33516130	2	64	theme	dense	523:527	arg1	geometries					538:547	pure 3D dense chitosan geometries	515:547	pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures	515:621	Porous chitosan scaffolds have been extensively studied and explored in bone generation, however it is still a challenge to obtain bioabsorbable orthopedic implants that involves pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures.
33516130	6	65	theme	different	1324:1332	arg1	conditions					1343:1352	different hydrated conditions	1324:1352	different hydrated conditions	1324:1352	The specimens were analysed in quasi-static conditions (less than 0.1 s-1) and medium strain rate conditions (200-800 s-1), both in dry state and in different hydrated conditions, in the latter case to approximate the in vivo implant conditions.
33516130	0	66	theme	hydrated	63:70	arg1	materials					93:101	dry and hydrated chitosan-based dense materials	55:101	dry and hydrated chitosan-based dense materials	55:101	Influence of strain rate on the mechanical behavior of dry and hydrated chitosan-based dense materials for bioabsorbable implant applications.
33516130	5	67	theme	customized	1127:1136	arg1	SHPB					1168:1171	SHPB	1168:1171	SHPB	1168:1171	The mechanical behavior of the two chitosan-based compositions was evaluated by uniaxial compression tests using a customized split-Hopkinson pressure bar (SHPB).
33516130	5	67	theme	customized	1127:1136	arg1	bar					1163:1165	a customized split-Hopkinson pressure bar	1125:1165	a customized split-Hopkinson pressure bar (SHPB)	1125:1172	The mechanical behavior of the two chitosan-based compositions was evaluated by uniaxial compression tests using a customized split-Hopkinson pressure bar (SHPB).
33516130	2	68	theme	pure	515:518	arg1	geometries					538:547	pure 3D dense chitosan geometries	515:547	pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures	515:621	Porous chitosan scaffolds have been extensively studied and explored in bone generation, however it is still a challenge to obtain bioabsorbable orthopedic implants that involves pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures.
33516130	5	69	theme	compositions	1062:1073	arg1	behavior					1027:1034	The mechanical behavior	1012:1034	The mechanical behavior of the two chitosan-based compositions	1012:1073	The mechanical behavior of the two chitosan-based compositions was evaluated by uniaxial compression tests using a customized split-Hopkinson pressure bar (SHPB).
33516130	0	70	theme	dense	87:91	arg1	materials					93:101	dry and hydrated chitosan-based dense materials	55:101	dry and hydrated chitosan-based dense materials	55:101	Influence of strain rate on the mechanical behavior of dry and hydrated chitosan-based dense materials for bioabsorbable implant applications.
33516130	5	71	theme	pressure	1154:1161	arg1	SHPB					1168:1171	SHPB	1168:1171	SHPB	1168:1171	The mechanical behavior of the two chitosan-based compositions was evaluated by uniaxial compression tests using a customized split-Hopkinson pressure bar (SHPB).
33516130	5	71	theme	pressure	1154:1161	arg1	bar					1163:1165	a customized split-Hopkinson pressure bar	1125:1165	a customized split-Hopkinson pressure bar (SHPB)	1125:1172	The mechanical behavior of the two chitosan-based compositions was evaluated by uniaxial compression tests using a customized split-Hopkinson pressure bar (SHPB).
33516130	8	72	theme	chitosan	1492:1499	arg1	blends					1501:1506	The chitosan blends	1488:1506	The chitosan blends	1488:1506	The chitosan blends present excellent ductility with an elastic perfect-plastic behavior in quasi-static conditions, with yield stresses around 40 MPa for the dry state, with a decay for 3 MPa after 48h hydration.
33516130	3	73	theme	chitosan-based	808:821	arg1	blocks					823:828	dense chitosan-based blocks	802:828	dense chitosan-based blocks	802:828	In this work, chitosan was blended with 10% glycerol and 10% glycerol + 10% biphasic mixture of calcium phosphate (70% hydroxyapatite with 30% β-tricalcium phosphate) to produce dense chitosan-based blocks, which were then shaped into rods.
33516130	5	74	theme	compression	1101:1111	arg1	tests					1113:1117	uniaxial compression tests	1092:1117	uniaxial compression tests using a customized split-Hopkinson pressure bar (SHPB)	1092:1172	The mechanical behavior of the two chitosan-based compositions was evaluated by uniaxial compression tests using a customized split-Hopkinson pressure bar (SHPB).
33516130	9	75	theme	stress	1755:1760	arg1	curves					1762:1767	the flow stress curves	1746:1767	the flow stress curves	1746:1767	An empirical model was proposed to describe the flow stress curves, with a good agreement with the experimental data, allowing future modelling of this material behavior.
33516130	9	76	theme	flow	1750:1753	arg1	curves					1762:1767	the flow stress curves	1746:1767	the flow stress curves	1746:1767	An empirical model was proposed to describe the flow stress curves, with a good agreement with the experimental data, allowing future modelling of this material behavior.
33516130	6	77	theme	implant	1401:1407	arg1	conditions					1409:1418	the in vivo implant conditions	1389:1418	the in vivo implant conditions	1389:1418	The specimens were analysed in quasi-static conditions (less than 0.1 s-1) and medium strain rate conditions (200-800 s-1), both in dry state and in different hydrated conditions, in the latter case to approximate the in vivo implant conditions.
33516130	2	78	theme	bone	408:411	arg1	generation					413:422	bone generation	408:422	bone generation	408:422	Porous chitosan scaffolds have been extensively studied and explored in bone generation, however it is still a challenge to obtain bioabsorbable orthopedic implants that involves pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures.
33516130	0	79	theme	rate	20:23	arg1	Influence					0:8	Influence	0:8	Influence of strain rate on the mechanical behavior of dry and hydrated chitosan-based dense materials for bioabsorbable implant applications.	0:141	Influence of strain rate on the mechanical behavior of dry and hydrated chitosan-based dense materials for bioabsorbable implant applications.
33516130	1	80	theme	advanced	298:305	arg1	structures					307:316	new advanced structures	294:316	new advanced structures	294:316	Chitosan has generated enormous interest in the scientific community because of its distinctive biological and physicochemical properties, which allow new advanced structures and applications.
33516130	3	81	with	hydroxyapatite	743:756	arg1	phosphate					780:788	30% β-tricalcium phosphate	763:788	30% β-tricalcium phosphate	763:788	In this work, chitosan was blended with 10% glycerol and 10% glycerol + 10% biphasic mixture of calcium phosphate (70% hydroxyapatite with 30% β-tricalcium phosphate) to produce dense chitosan-based blocks, which were then shaped into rods.
33516130	3	82	theme	%	666:666	arg1	glycerol					668:675	10% glycerol	664:675	10% glycerol	664:675	In this work, chitosan was blended with 10% glycerol and 10% glycerol + 10% biphasic mixture of calcium phosphate (70% hydroxyapatite with 30% β-tricalcium phosphate) to produce dense chitosan-based blocks, which were then shaped into rods.
33516130	8	83	theme	perfect-plastic	1552:1566	arg1	behavior					1568:1575	an elastic perfect-plastic behavior	1541:1575	an elastic perfect-plastic behavior in quasi-static conditions	1541:1602	The chitosan blends present excellent ductility with an elastic perfect-plastic behavior in quasi-static conditions, with yield stresses around 40 MPa for the dry state, with a decay for 3 MPa after 48h hydration.
33516130	6	84	dep	in	1393:1394	arg1	vivo					1396:1399	vivo	1396:1399	vivo	1396:1399	The specimens were analysed in quasi-static conditions (less than 0.1 s-1) and medium strain rate conditions (200-800 s-1), both in dry state and in different hydrated conditions, in the latter case to approximate the in vivo implant conditions.
33516130	5	85	theme	mechanical	1016:1025	arg1	behavior					1027:1034	The mechanical behavior	1012:1034	The mechanical behavior of the two chitosan-based compositions	1012:1073	The mechanical behavior of the two chitosan-based compositions was evaluated by uniaxial compression tests using a customized split-Hopkinson pressure bar (SHPB).
33516130	2	86	theme	orthopedic	481:490	arg1	implants					492:499	bioabsorbable orthopedic implants	467:499	bioabsorbable orthopedic implants that involves pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures	467:621	Porous chitosan scaffolds have been extensively studied and explored in bone generation, however it is still a challenge to obtain bioabsorbable orthopedic implants that involves pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures.
33516130	3	87	theme	%	683:683	arg1	glycerol					685:692	10% glycerol	681:692	10% glycerol	681:692	In this work, chitosan was blended with 10% glycerol and 10% glycerol + 10% biphasic mixture of calcium phosphate (70% hydroxyapatite with 30% β-tricalcium phosphate) to produce dense chitosan-based blocks, which were then shaped into rods.
33516130	2	88	from	difficulties	569:580	arg1	shaping					599:605	shaping	599:605	shaping	599:605	Porous chitosan scaffolds have been extensively studied and explored in bone generation, however it is still a challenge to obtain bioabsorbable orthopedic implants that involves pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures.
33516130	2	88	from	difficulties	569:580	arg1	producing					585:593	producing	585:593	producing	585:593	Porous chitosan scaffolds have been extensively studied and explored in bone generation, however it is still a challenge to obtain bioabsorbable orthopedic implants that involves pure 3D dense chitosan geometries due to the inherent difficulties in producing and shaping such structures.
33516130	1	89	theme	biological	239:248	arg1	properties					270:279	its distinctive biological and physicochemical properties	223:279	its distinctive biological and physicochemical properties	223:279	Chitosan has generated enormous interest in the scientific community because of its distinctive biological and physicochemical properties, which allow new advanced structures and applications.
33516130	7	90	theme	intended	1466:1473	arg1	application					1475:1485	the intended application	1462:1485	the intended application	1462:1485	The results showed promising results for the intended application.
33516130	0	91	theme	bioabsorbable	107:119	arg1	applications					129:140	bioabsorbable implant applications	107:140	bioabsorbable implant applications	107:140	Influence of strain rate on the mechanical behavior of dry and hydrated chitosan-based dense materials for bioabsorbable implant applications.
33516130	3	92	theme	%	698:698	arg1	mixture					709:715	10% biphasic mixture	696:715	10% biphasic mixture	696:715	In this work, chitosan was blended with 10% glycerol and 10% glycerol + 10% biphasic mixture of calcium phosphate (70% hydroxyapatite with 30% β-tricalcium phosphate) to produce dense chitosan-based blocks, which were then shaped into rods.
33516130	1	93	theme	physicochemical	254:268	arg1	properties					270:279	its distinctive biological and physicochemical properties	223:279	its distinctive biological and physicochemical properties	223:279	Chitosan has generated enormous interest in the scientific community because of its distinctive biological and physicochemical properties, which allow new advanced structures and applications.
33152359	0	0	theme	antioxidant	93:103	arg1	xylooligosaccharides					166:185	anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides	75:185	anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides	75:185	Characterization of Aspergillus niger MK981235 xylanase with extraction of anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides.
33152359	3	1	theme	niger	773:777	arg1	xylanase					788:795	A. niger MK981235 xylanase	770:795	A. niger MK981235 xylanase	770:795	A. niger MK981235 xylanase showed the highest activity 6.60 U·ml-1 at 50 °C with 1.5% xylan.
33152359	7	2	theme	protective	1468:1477	arg1	role					1479:1482	remarkable in vivo protective role	1449:1482	remarkable in vivo protective role	1449:1482	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	5	3	dep	thermodynamics	1007:1020	arg1	thermodynamics					1007:1020	A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation)	980:1086	A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation)	980:1086	Moreover, A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation) were determined to be 21.95 and 39.51 KJ·mol-1, respectively.
33152359	5	3	dep	thermodynamics	1007:1020	arg1	Ea					1022:1023	Ea	1022:1023	Ea (activation energy)	1022:1043	Moreover, A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation) were determined to be 21.95 and 39.51 KJ·mol-1, respectively.
33152359	5	3	dep	thermodynamics	1007:1020	arg1	energy					1037:1042	activation energy	1026:1042	activation energy	1026:1042	Moreover, A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation) were determined to be 21.95 and 39.51 KJ·mol-1, respectively.
33152359	5	3	dep	thermodynamics	1007:1020	arg1	Ed					1049:1050	Ed	1049:1050	Ed (activation energy of denaturation)	1049:1086	Moreover, A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation) were determined to be 21.95 and 39.51 KJ·mol-1, respectively.
33152359	7	4	theme	tissue	1631:1636	arg1	MDA					1646:1648	MDA	1646:1648	MDA	1646:1648	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	7	4	theme	tissue	1631:1636	arg1	makers					1638:1643	tissue makers	1631:1643	tissue makers (MDA and GSH)	1631:1657	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	7	4	theme	tissue	1631:1636	arg1	GSH					1654:1656	GSH	1654:1656	GSH	1654:1656	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	7	5	theme	function	1577:1584	arg1	markers					1586:1592	liver function markers	1571:1592	liver function markers (AST, ALT, Alb, total bilirubin)	1571:1625	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	2	6	theme	saccharification	518:533	arg1	conditions					535:544	different saccharification conditions	508:544	different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent	508:767	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	2	7	theme	different	706:714	arg1	constituents					732:743	different monosaccharides constituents	706:743	different monosaccharides constituents	706:743	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	6	8	theme	growth	1180:1185	arg1	effect					1172:1177	The highest prebiotic effect	1150:1177	The highest prebiotic effect (growth promation)	1150:1196	The highest prebiotic effect (growth promation) was exerted by the central MS XOS on Lactobacillus plantarum and Lactobacillus rhamnosus (125 and 135.3%, respectively).
33152359	6	8	theme	growth	1180:1185	arg1	promation					1187:1195	growth promation	1180:1195	growth promation	1180:1195	The highest prebiotic effect (growth promation) was exerted by the central MS XOS on Lactobacillus plantarum and Lactobacillus rhamnosus (125 and 135.3%, respectively).
33152359	7	9	from	changes	1547:1553	arg1	MDA					1646:1648	MDA	1646:1648	MDA	1646:1648	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	7	9	from	changes	1547:1553	arg1	makers					1638:1643	tissue makers	1631:1643	tissue makers (MDA and GSH)	1631:1657	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	7	9	from	changes	1547:1553	arg1	markers					1586:1592	liver function markers	1571:1592	liver function markers (AST, ALT, Alb, total bilirubin)	1571:1625	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	7	9	from	changes	1547:1553	arg1	GSH					1654:1656	GSH	1654:1656	GSH	1654:1656	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	7	9	from	changes	1547:1553	arg1	weight					1563:1568	body weight	1558:1568	body weight	1558:1568	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	0	10	theme	hypocholesterolemic	106:124	arg1	xylooligosaccharides					166:185	anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides	75:185	anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides	75:185	Characterization of Aspergillus niger MK981235 xylanase with extraction of anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides.
33152359	7	11	theme	hepatic	1496:1502	arg1	toxicity					1504:1511	the hepatic toxicity	1492:1511	the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH)	1492:1657	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	3	12	theme	1.5	851:853	arg1	%					854:854	%	854:854	%	854:854	A. niger MK981235 xylanase showed the highest activity 6.60 U·ml-1 at 50 °C with 1.5% xylan.
33152359	7	13	theme	MS	1337:1338	arg1	XOS					1340:1342	the central MS XOS	1325:1342	the central MS XOS	1325:1342	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	7	14	dep	in	1460:1461	arg1	vivo					1463:1466	vivo	1463:1466	vivo	1463:1466	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	7	15	dep	makers	1638:1643	arg1	MDA					1646:1648	MDA	1646:1648	MDA	1646:1648	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	7	15	dep	makers	1638:1643	arg1	makers					1638:1643	tissue makers	1631:1643	tissue makers (MDA and GSH)	1631:1657	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	7	15	dep	makers	1638:1643	arg1	GSH					1654:1656	GSH	1654:1656	GSH	1654:1656	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	2	16	theme	XOS	630:632	arg1	extraction					593:602	extraction	593:602	extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent	593:767	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	6	17	theme	MS	1225:1226	arg1	XOS					1228:1230	the central MS XOS	1213:1230	the central MS XOS	1213:1230	The highest prebiotic effect (growth promation) was exerted by the central MS XOS on Lactobacillus plantarum and Lactobacillus rhamnosus (125 and 135.3%, respectively).
33152359	7	18	theme	liver	1571:1575	arg1	markers					1586:1592	liver function markers	1571:1592	liver function markers (AST, ALT, Alb, total bilirubin)	1571:1625	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	0	19	theme	prebiotic	130:138	arg1	xylooligosaccharides					166:185	anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides	75:185	anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides	75:185	Characterization of Aspergillus niger MK981235 xylanase with extraction of anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides.
33152359	5	20	dep	Ed	1049:1050	arg1	energy					1064:1069	activation energy	1053:1069	activation energy of denaturation	1053:1085	Moreover, A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation) were determined to be 21.95 and 39.51 KJ·mol-1, respectively.
33152359	6	21	theme	central	1217:1223	arg1	XOS					1228:1230	the central MS XOS	1213:1230	the central MS XOS	1213:1230	The highest prebiotic effect (growth promation) was exerted by the central MS XOS on Lactobacillus plantarum and Lactobacillus rhamnosus (125 and 135.3%, respectively).
33152359	2	22	with	XOS	630:632	arg1	constituents					732:743	different monosaccharides constituents	706:743	different monosaccharides constituents	706:743	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	2	23	theme	central	558:564	arg1	CCD					584:586	CCD	584:586	CCD	584:586	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	2	23	theme	central	558:564	arg1	design					576:581	central composite design	558:581	central composite design (CCD)	558:587	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	1	24	theme	active	392:397	arg1	XOS					421:423	XOS	421:423	XOS	421:423	The object of this study was to utilize agro-industrial waste Corchorus olitorius stems (molokhia stems, MS) as substrate, for Aspergillus niger MK981235 xylanase production and as source of biologically active xylooligosaccharides (XOS).
33152359	1	24	theme	active	392:397	arg1	xylooligosaccharides					399:418	biologically active xylooligosaccharides	379:418	biologically active xylooligosaccharides (XOS)	379:424	The object of this study was to utilize agro-industrial waste Corchorus olitorius stems (molokhia stems, MS) as substrate, for Aspergillus niger MK981235 xylanase production and as source of biologically active xylooligosaccharides (XOS).
33152359	2	25	theme	different	508:516	arg1	conditions					535:544	different saccharification conditions	508:544	different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent	508:767	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	1	26	theme	study	207:211	arg1	object					192:197	The object	188:197	The object of this study	188:211	The object of this study was to utilize agro-industrial waste Corchorus olitorius stems (molokhia stems, MS) as substrate, for Aspergillus niger MK981235 xylanase production and as source of biologically active xylooligosaccharides (XOS).
33152359	2	27	theme	niger	478:482	arg1	xylanase					493:500	Aspergillus niger MK981235 xylanase	466:500	Aspergillus niger MK981235 xylanase	466:500	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	1	28	theme	xylooligosaccharides	399:418	arg1	source					369:374	source	369:374	source of biologically active xylooligosaccharides (XOS)	369:424	The object of this study was to utilize agro-industrial waste Corchorus olitorius stems (molokhia stems, MS) as substrate, for Aspergillus niger MK981235 xylanase production and as source of biologically active xylooligosaccharides (XOS).
33152359	7	29	dep	markers	1586:1592	arg1	AST					1595:1597	AST	1595:1597	AST	1595:1597	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	7	29	dep	markers	1586:1592	arg1	Alb					1605:1607	Alb	1605:1607	Alb	1605:1607	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	7	29	dep	markers	1586:1592	arg1	bilirubin					1616:1624	total bilirubin	1610:1624	total bilirubin	1610:1624	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	7	29	dep	markers	1586:1592	arg1	ALT					1600:1602	ALT	1600:1602	ALT	1600:1602	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	2	30	theme	Aspergillus	466:476	arg1	xylanase					493:500	Aspergillus niger MK981235 xylanase	466:500	Aspergillus niger MK981235 xylanase	466:500	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	7	31	theme	remarkable	1449:1458	arg1	role					1479:1482	remarkable in vivo protective role	1449:1482	remarkable in vivo protective role	1449:1482	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	3	32	theme	MK981235	779:786	arg1	xylanase					788:795	A. niger MK981235 xylanase	770:795	A. niger MK981235 xylanase	770:795	A. niger MK981235 xylanase showed the highest activity 6.60 U·ml-1 at 50 °C with 1.5% xylan.
33152359	3	33	theme	%	854:854	arg1	xylan					856:860	1.5% xylan	851:860	1.5% xylan	851:860	A. niger MK981235 xylanase showed the highest activity 6.60 U·ml-1 at 50 °C with 1.5% xylan.
33152359	7	34	theme	in	1460:1461	arg1	role					1479:1482	remarkable in vivo protective role	1449:1482	remarkable in vivo protective role	1449:1482	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	0	35	theme	niger	32:36	arg1	xylanase					47:54	Aspergillus niger MK981235 xylanase	20:54	Aspergillus niger MK981235 xylanase with extraction of anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides	20:185	Characterization of Aspergillus niger MK981235 xylanase with extraction of anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides.
33152359	6	36	dep	Lactobacillus	1263:1275	arg1	rhamnosus					1277:1285	Lactobacillus rhamnosus	1263:1285	Lactobacillus rhamnosus	1263:1285	The highest prebiotic effect (growth promation) was exerted by the central MS XOS on Lactobacillus plantarum and Lactobacillus rhamnosus (125 and 135.3%, respectively).
33152359	0	37	theme	olitorius	150:158	arg1	xylooligosaccharides					166:185	anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides	75:185	anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides	75:185	Characterization of Aspergillus niger MK981235 xylanase with extraction of anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides.
33152359	2	38	theme	monosaccharides	716:730	arg1	constituents					732:743	different monosaccharides constituents	706:743	different monosaccharides constituents	706:743	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	3	39	with	50 °C	840:844	arg1	xylan					856:860	1.5% xylan	851:860	1.5% xylan	851:860	A. niger MK981235 xylanase showed the highest activity 6.60 U·ml-1 at 50 °C with 1.5% xylan.
33152359	7	40	theme	central	1329:1335	arg1	XOS					1340:1342	the central MS XOS	1325:1342	the central MS XOS	1325:1342	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	0	41	theme	Aspergillus	20:30	arg1	xylanase					47:54	Aspergillus niger MK981235 xylanase	20:54	Aspergillus niger MK981235 xylanase with extraction of anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides	20:185	Characterization of Aspergillus niger MK981235 xylanase with extraction of anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides.
33152359	3	42	theme	highest	808:814	arg1	6.60 U·ml-1					825:835	the highest activity 6.60 U·ml-1	804:835	the highest activity 6.60 U·ml-1	804:835	A. niger MK981235 xylanase showed the highest activity 6.60 U·ml-1 at 50 °C with 1.5% xylan.
33152359	0	43	theme	Corchorus	140:148	arg1	xylooligosaccharides					166:185	anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides	75:185	anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides	75:185	Characterization of Aspergillus niger MK981235 xylanase with extraction of anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides.
33152359	7	44	theme	body	1558:1561	arg1	weight					1563:1568	body weight	1558:1568	body weight	1558:1568	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	5	45	theme	activation	1026:1035	arg1	Ea					1022:1023	Ea	1022:1023	Ea (activation energy)	1022:1043	Moreover, A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation) were determined to be 21.95 and 39.51 KJ·mol-1, respectively.
33152359	5	45	theme	activation	1026:1035	arg1	energy					1037:1042	activation energy	1026:1042	activation energy	1026:1042	Moreover, A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation) were determined to be 21.95 and 39.51 KJ·mol-1, respectively.
33152359	1	46	theme	agro-industrial	228:242	arg1	stems					270:274	agro-industrial waste Corchorus olitorius stems	228:274	agro-industrial waste Corchorus olitorius stems (molokhia stems, MS)	228:295	The object of this study was to utilize agro-industrial waste Corchorus olitorius stems (molokhia stems, MS) as substrate, for Aspergillus niger MK981235 xylanase production and as source of biologically active xylooligosaccharides (XOS).
33152359	0	47	theme	xylanase	47:54	arg1	Characterization					0:15	Characterization	0:15	Characterization of Aspergillus niger MK981235 xylanase with extraction of anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides.	0:186	Characterization of Aspergillus niger MK981235 xylanase with extraction of anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides.
33152359	3	48	theme	activity	816:823	arg1	6.60 U·ml-1					825:835	the highest activity 6.60 U·ml-1	804:835	the highest activity 6.60 U·ml-1	804:835	A. niger MK981235 xylanase showed the highest activity 6.60 U·ml-1 at 50 °C with 1.5% xylan.
33152359	0	49	theme	xylooligosaccharides	166:185	arg1	extraction					61:70	extraction	61:70	extraction of anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides	61:185	Characterization of Aspergillus niger MK981235 xylanase with extraction of anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides.
33152359	5	50	theme	denaturation	1074:1085	arg1	energy					1064:1069	activation energy	1053:1069	activation energy of denaturation	1053:1085	Moreover, A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation) were determined to be 21.95 and 39.51 KJ·mol-1, respectively.
33152359	1	51	theme	waste	244:248	arg1	stems					270:274	agro-industrial waste Corchorus olitorius stems	228:274	agro-industrial waste Corchorus olitorius stems (molokhia stems, MS)	228:295	The object of this study was to utilize agro-industrial waste Corchorus olitorius stems (molokhia stems, MS) as substrate, for Aspergillus niger MK981235 xylanase production and as source of biologically active xylooligosaccharides (XOS).
33152359	2	52	dep	XOS	630:632	arg1	anti-hepatotoxic					635:650	anti-hepatotoxic	635:650	anti-hepatotoxic	635:650	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	2	52	dep	XOS	630:632	arg1	prebiotic					690:698	prebiotic	690:698	prebiotic	690:698	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	2	52	dep	XOS	630:632	arg1	percent					761:767	percent	761:767	percent	761:767	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	2	52	dep	XOS	630:632	arg1	antioxidant					653:663	antioxidant	653:663	antioxidant	653:663	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	2	52	dep	XOS	630:632	arg1	hypocholesterolemic					666:684	hypocholesterolemic	666:684	hypocholesterolemic	666:684	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	2	52	dep	XOS	630:632	arg1	XOS					630:632	15 biologically active XOS	607:632	15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent	607:767	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	2	52	dep	XOS	630:632	arg1	composition					745:755	composition	745:755	composition	745:755	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	1	53	theme	Aspergillus	315:325	arg1	production					351:360	Aspergillus niger MK981235 xylanase production	315:360	Aspergillus niger MK981235 xylanase production	315:360	The object of this study was to utilize agro-industrial waste Corchorus olitorius stems (molokhia stems, MS) as substrate, for Aspergillus niger MK981235 xylanase production and as source of biologically active xylooligosaccharides (XOS).
33152359	0	54	theme	stems	160:164	arg1	xylooligosaccharides					166:185	anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides	75:185	anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides	75:185	Characterization of Aspergillus niger MK981235 xylanase with extraction of anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides.
33152359	1	55	theme	Corchorus	250:258	arg1	stems					270:274	agro-industrial waste Corchorus olitorius stems	228:274	agro-industrial waste Corchorus olitorius stems (molokhia stems, MS)	228:295	The object of this study was to utilize agro-industrial waste Corchorus olitorius stems (molokhia stems, MS) as substrate, for Aspergillus niger MK981235 xylanase production and as source of biologically active xylooligosaccharides (XOS).
33152359	2	56	theme	xylanase	493:500	arg1	utilization					451:461	utilization	451:461	utilization of Aspergillus niger MK981235 xylanase	451:500	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	2	57	theme	composite	566:574	arg1	CCD					584:586	CCD	584:586	CCD	584:586	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	2	57	theme	composite	566:574	arg1	design					576:581	central composite design	558:581	central composite design (CCD)	558:587	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	2	58	theme	active	623:628	arg1	XOS					630:632	15 biologically active XOS	607:632	15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent	607:767	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	2	58	theme	active	623:628	arg1	composition					745:755	composition	745:755	composition	745:755	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	2	58	theme	active	623:628	arg1	percent					761:767	percent	761:767	percent	761:767	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	1	59	dep	stems	270:274	arg1	MS					293:294	MS	293:294	MS	293:294	The object of this study was to utilize agro-industrial waste Corchorus olitorius stems (molokhia stems, MS) as substrate, for Aspergillus niger MK981235 xylanase production and as source of biologically active xylooligosaccharides (XOS).
33152359	1	59	dep	stems	270:274	arg1	stems					286:290	molokhia stems	277:290	molokhia stems	277:290	The object of this study was to utilize agro-industrial waste Corchorus olitorius stems (molokhia stems, MS) as substrate, for Aspergillus niger MK981235 xylanase production and as source of biologically active xylooligosaccharides (XOS).
33152359	7	60	theme	antioxidant	1391:1401	arg1	activities					1403:1412	antioxidant activities 74.7 and 92%	1391:1425	activities	1403:1412	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	1	61	dep	Aspergillus	315:325	arg1	niger					327:331	niger	327:331	niger	327:331	The object of this study was to utilize agro-industrial waste Corchorus olitorius stems (molokhia stems, MS) as substrate, for Aspergillus niger MK981235 xylanase production and as source of biologically active xylooligosaccharides (XOS).
33152359	6	62	dep	Lactobacillus	1235:1247	arg1	plantarum					1249:1257	Lactobacillus plantarum	1235:1257	Lactobacillus plantarum	1235:1257	The highest prebiotic effect (growth promation) was exerted by the central MS XOS on Lactobacillus plantarum and Lactobacillus rhamnosus (125 and 135.3%, respectively).
33152359	6	62	dep	Lactobacillus	1235:1247	arg1	%					1301:1301	135.3%	1296:1301	135.3%	1296:1301	The highest prebiotic effect (growth promation) was exerted by the central MS XOS on Lactobacillus plantarum and Lactobacillus rhamnosus (125 and 135.3%, respectively).
33152359	6	62	dep	Lactobacillus	1235:1247	arg1	125					1288:1290	125	1288:1290	125	1288:1290	The highest prebiotic effect (growth promation) was exerted by the central MS XOS on Lactobacillus plantarum and Lactobacillus rhamnosus (125 and 135.3%, respectively).
33152359	2	63	theme	MK981235	484:491	arg1	xylanase					493:500	Aspergillus niger MK981235 xylanase	466:500	Aspergillus niger MK981235 xylanase	466:500	This study succeeded in utilization of Aspergillus niger MK981235 xylanase under different saccharification conditions designed by central composite design (CCD) for extraction of 15 biologically active XOS (anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic) with different monosaccharides constituents composition and percent.
33152359	1	64	theme	MK981235	333:340	arg1	production					351:360	Aspergillus niger MK981235 xylanase production	315:360	Aspergillus niger MK981235 xylanase production	315:360	The object of this study was to utilize agro-industrial waste Corchorus olitorius stems (molokhia stems, MS) as substrate, for Aspergillus niger MK981235 xylanase production and as source of biologically active xylooligosaccharides (XOS).
33152359	5	65	theme	niger	983:987	arg1	thermodynamics					1007:1020	A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation)	980:1086	A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation)	980:1086	Moreover, A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation) were determined to be 21.95 and 39.51 KJ·mol-1, respectively.
33152359	5	65	theme	niger	983:987	arg1	Ea					1022:1023	Ea	1022:1023	Ea (activation energy)	1022:1043	Moreover, A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation) were determined to be 21.95 and 39.51 KJ·mol-1, respectively.
33152359	5	65	theme	niger	983:987	arg1	Ed					1049:1050	Ed	1049:1050	Ed (activation energy of denaturation)	1049:1086	Moreover, A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation) were determined to be 21.95 and 39.51 KJ·mol-1, respectively.
33152359	1	66	theme	xylanase	342:349	arg1	production					351:360	Aspergillus niger MK981235 xylanase production	315:360	Aspergillus niger MK981235 xylanase production	315:360	The object of this study was to utilize agro-industrial waste Corchorus olitorius stems (molokhia stems, MS) as substrate, for Aspergillus niger MK981235 xylanase production and as source of biologically active xylooligosaccharides (XOS).
33152359	1	67	theme	olitorius	260:268	arg1	stems					270:274	agro-industrial waste Corchorus olitorius stems	228:274	agro-industrial waste Corchorus olitorius stems (molokhia stems, MS)	228:295	The object of this study was to utilize agro-industrial waste Corchorus olitorius stems (molokhia stems, MS) as substrate, for Aspergillus niger MK981235 xylanase production and as source of biologically active xylooligosaccharides (XOS).
33152359	7	68	theme	lithium	1516:1522	arg1	carbonate					1524:1532	lithium carbonate	1516:1532	lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH)	1516:1657	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	5	69	theme	activation	1053:1062	arg1	energy					1064:1069	activation energy	1053:1069	activation energy of denaturation	1053:1085	Moreover, A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation) were determined to be 21.95 and 39.51 KJ·mol-1, respectively.
33152359	7	70	theme	total	1610:1614	arg1	AST					1595:1597	AST	1595:1597	AST	1595:1597	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	7	70	theme	total	1610:1614	arg1	bilirubin					1616:1624	total bilirubin	1610:1624	total bilirubin	1610:1624	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	5	71	theme	xylanase	998:1005	arg1	thermodynamics					1007:1020	A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation)	980:1086	A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation)	980:1086	Moreover, A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation) were determined to be 21.95 and 39.51 KJ·mol-1, respectively.
33152359	5	71	theme	xylanase	998:1005	arg1	Ea					1022:1023	Ea	1022:1023	Ea (activation energy)	1022:1043	Moreover, A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation) were determined to be 21.95 and 39.51 KJ·mol-1, respectively.
33152359	5	71	theme	xylanase	998:1005	arg1	Ed					1049:1050	Ed	1049:1050	Ed (activation energy of denaturation)	1049:1086	Moreover, A. niger MK981235 xylanase thermodynamics Ea (activation energy) and Ed (activation energy of denaturation) were determined to be 21.95 and 39.51 KJ·mol-1, respectively.
33152359	0	72	theme	anti-hepatotoxic	75:90	arg1	xylooligosaccharides					166:185	anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides	75:185	anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides	75:185	Characterization of Aspergillus niger MK981235 xylanase with extraction of anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides.
33152359	7	73	theme	carbonate	1524:1532	arg1	toxicity					1504:1511	the hepatic toxicity	1492:1511	the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH)	1492:1657	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	7	74	theme	highest	1357:1363	arg1	reduction					1377:1385	the highest cholesterol reduction	1353:1385	the highest cholesterol reduction	1353:1385	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	6	75	theme	prebiotic	1162:1170	arg1	effect					1172:1177	The highest prebiotic effect	1150:1177	The highest prebiotic effect (growth promation)	1150:1196	The highest prebiotic effect (growth promation) was exerted by the central MS XOS on Lactobacillus plantarum and Lactobacillus rhamnosus (125 and 135.3%, respectively).
33152359	6	75	theme	prebiotic	1162:1170	arg1	promation					1187:1195	growth promation	1180:1195	growth promation	1180:1195	The highest prebiotic effect (growth promation) was exerted by the central MS XOS on Lactobacillus plantarum and Lactobacillus rhamnosus (125 and 135.3%, respectively).
33152359	3	76	theme	A.	770:771	arg1	xylanase					788:795	A. niger MK981235 xylanase	770:795	A. niger MK981235 xylanase	770:795	A. niger MK981235 xylanase showed the highest activity 6.60 U·ml-1 at 50 °C with 1.5% xylan.
33152359	7	77	theme	cholesterol	1365:1375	arg1	reduction					1377:1385	the highest cholesterol reduction	1353:1385	the highest cholesterol reduction	1353:1385	Also, the central MS XOS, exerted the highest cholesterol reduction and antioxidant activities 74.7 and 92%, respectively, showed remarkable in vivo protective role against the hepatic toxicity of lithium carbonate evaluated by changes in body weight, liver function markers (AST, ALT, Alb, total bilirubin) and tissue makers (MDA and GSH).
33152359	0	78	with	xylanase	47:54	arg1	extraction					61:70	extraction	61:70	extraction of anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides	61:185	Characterization of Aspergillus niger MK981235 xylanase with extraction of anti-hepatotoxic, antioxidant, hypocholesterolemic and prebiotic Corchorus olitorius stems xylooligosaccharides.
33152359	6	79	theme	highest	1154:1160	arg1	effect					1172:1177	The highest prebiotic effect	1150:1177	The highest prebiotic effect (growth promation)	1150:1196	The highest prebiotic effect (growth promation) was exerted by the central MS XOS on Lactobacillus plantarum and Lactobacillus rhamnosus (125 and 135.3%, respectively).
33152359	6	79	theme	highest	1154:1160	arg1	promation					1187:1195	growth promation	1180:1195	growth promation	1180:1195	The highest prebiotic effect (growth promation) was exerted by the central MS XOS on Lactobacillus plantarum and Lactobacillus rhamnosus (125 and 135.3%, respectively).
33152359	1	80	theme	molokhia	277:284	arg1	MS					293:294	MS	293:294	MS	293:294	The object of this study was to utilize agro-industrial waste Corchorus olitorius stems (molokhia stems, MS) as substrate, for Aspergillus niger MK981235 xylanase production and as source of biologically active xylooligosaccharides (XOS).
33152359	1	80	theme	molokhia	277:284	arg1	stems					286:290	molokhia stems	277:290	molokhia stems	277:290	The object of this study was to utilize agro-industrial waste Corchorus olitorius stems (molokhia stems, MS) as substrate, for Aspergillus niger MK981235 xylanase production and as source of biologically active xylooligosaccharides (XOS).
32693272	4	0	theme	adsorption	493:502	arg1	capacity					504:511	the maximum adsorption capacity	481:511	the maximum adsorption capacity of Cu2+ and Pb2+	481:528	Under optimal conditions, the maximum adsorption capacity of Cu2+ and Pb2+ were 68.76 mg/g and 240.69 mg/g, respectively.
32693272	4	0	theme	adsorption	493:502	arg1	68.76 mg/g					535:544	68.76 mg/g	535:544	68.76 mg/g	535:544	Under optimal conditions, the maximum adsorption capacity of Cu2+ and Pb2+ were 68.76 mg/g and 240.69 mg/g, respectively.
32693272	8	1	theme	adsorption	1054:1063	arg1	capacity					1065:1072	good adsorption capacity	1049:1072	good adsorption capacity	1049:1072	Furthermore, the adsorbent still maintained good adsorption capacity after 5 cycles of adsorption-regeneration.
32693272	7	2	theme	ion	934:936	arg1	process					947:953	the ion exchange process	930:953	the ion exchange process	930:953	The chemical adsorption accompanying the ion exchange process was confirmed as the staple adsorption mechanism.
32693272	0	3	theme	oxide	88:92	arg1	hydrogel					94:101	polyacrylamide/graphene oxide hydrogel	64:101	polyacrylamide/graphene oxide hydrogel for the adsorption of heavy metal ion	64:139	Preparation of a novel bio-adsorbent of sodium alginate grafted polyacrylamide/graphene oxide hydrogel for the adsorption of heavy metal ion.
32693272	4	4	theme	Cu2+	516:519	arg1	capacity					504:511	the maximum adsorption capacity	481:511	the maximum adsorption capacity of Cu2+ and Pb2+	481:528	Under optimal conditions, the maximum adsorption capacity of Cu2+ and Pb2+ were 68.76 mg/g and 240.69 mg/g, respectively.
32693272	4	4	theme	Cu2+	516:519	arg1	68.76 mg/g					535:544	68.76 mg/g	535:544	68.76 mg/g	535:544	Under optimal conditions, the maximum adsorption capacity of Cu2+ and Pb2+ were 68.76 mg/g and 240.69 mg/g, respectively.
32693272	5	5	theme	pseudo-second-order	648:666	arg1	model					676:680	the pseudo-second-order kinetic model	644:680	the pseudo-second-order kinetic model	644:680	In addition, the research of kinetics and isotherms displayed that the pseudo-second-order kinetic model and the Langmuir isotherm model fitted the data well.
32693272	8	6	theme	good	1049:1052	arg1	capacity					1065:1072	good adsorption capacity	1049:1072	good adsorption capacity	1049:1072	Furthermore, the adsorbent still maintained good adsorption capacity after 5 cycles of adsorption-regeneration.
32693272	3	7	dep	increased	387:395	arg1	tripled					407:413	tripled	407:413	tripled	407:413	After modification, the BET surface area increased more than tripled, which was consistent with SEM results.
32693272	6	8	theme	chemical	826:833	arg1	adsorption					835:844	chemical adsorption	826:844	chemical adsorption	826:844	After further research, the different adsorption mechanism including physical adsorption, chemical adsorption and electrostatic interactions were discussed.
32693272	9	9	theme	SA-PAM/GO	1132:1140	arg1	composites					1151:1160	the SA-PAM/GO hydrogel composites	1128:1160	the SA-PAM/GO hydrogel composites	1128:1160	Therefore, the SA-PAM/GO hydrogel composites have potential to remove the heavy metal ions from water body effectively.
32693272	3	10	theme	BET	370:372	arg1	area					382:385	the BET surface area	366:385	the BET surface area	366:385	After modification, the BET surface area increased more than tripled, which was consistent with SEM results.
32693272	9	11	attach	remove	1180:1185	arg2	potential					1167:1175	potential	1167:1175	potential to remove the heavy metal ions from water body effectively	1167:1234	Therefore, the SA-PAM/GO hydrogel composites have potential to remove the heavy metal ions from water body effectively.
32693272	9	11	attach	remove	1180:1185	arg1	body					1219:1222	water body	1213:1222	water body	1213:1222	Therefore, the SA-PAM/GO hydrogel composites have potential to remove the heavy metal ions from water body effectively.
32693272	8	12	theme	adsorption-regeneration	1092:1114	arg1	5 cycles					1080:1087	5 cycles	1080:1087	5 cycles of adsorption-regeneration	1080:1114	Furthermore, the adsorbent still maintained good adsorption capacity after 5 cycles of adsorption-regeneration.
32693272	9	13	theme	hydrogel	1142:1149	arg1	composites					1151:1160	the SA-PAM/GO hydrogel composites	1128:1160	the SA-PAM/GO hydrogel composites	1128:1160	Therefore, the SA-PAM/GO hydrogel composites have potential to remove the heavy metal ions from water body effectively.
32693272	9	14	contain	have	1162:1165	arg2	potential					1167:1175	potential	1167:1175	potential to remove the heavy metal ions from water body effectively	1167:1234	Therefore, the SA-PAM/GO hydrogel composites have potential to remove the heavy metal ions from water body effectively.
32693272	9	14	contain	have	1162:1165	arg1	composites					1151:1160	the SA-PAM/GO hydrogel composites	1128:1160	the SA-PAM/GO hydrogel composites	1128:1160	Therefore, the SA-PAM/GO hydrogel composites have potential to remove the heavy metal ions from water body effectively.
32693272	5	15	theme	kinetics	606:613	arg1	research					594:601	the research	590:601	the research of kinetics and isotherms	590:627	In addition, the research of kinetics and isotherms displayed that the pseudo-second-order kinetic model and the Langmuir isotherm model fitted the data well.
32693272	7	16	theme	chemical	897:904	arg1	adsorption					906:915	The chemical adsorption	893:915	The chemical adsorption accompanying the ion exchange process	893:953	The chemical adsorption accompanying the ion exchange process was confirmed as the staple adsorption mechanism.
32693272	7	16	theme	chemical	897:904	arg1	mechanism					994:1002	the staple adsorption mechanism	972:1002	the staple adsorption mechanism	972:1002	The chemical adsorption accompanying the ion exchange process was confirmed as the staple adsorption mechanism.
32693272	1	17	theme	SA-PAM/GO	170:178	arg1	composites					189:198	SA-PAM/GO hydrogel composites	170:198	SA-PAM/GO hydrogel composites	170:198	A novel bio-adsorbent named SA-PAM/GO hydrogel composites was synthesized through free radical polymerization.
32693272	4	18	theme	maximum	485:491	arg1	capacity					504:511	the maximum adsorption capacity	481:511	the maximum adsorption capacity of Cu2+ and Pb2+	481:528	Under optimal conditions, the maximum adsorption capacity of Cu2+ and Pb2+ were 68.76 mg/g and 240.69 mg/g, respectively.
32693272	4	18	theme	maximum	485:491	arg1	68.76 mg/g					535:544	68.76 mg/g	535:544	68.76 mg/g	535:544	Under optimal conditions, the maximum adsorption capacity of Cu2+ and Pb2+ were 68.76 mg/g and 240.69 mg/g, respectively.
32693272	5	19	theme	isotherms	619:627	arg1	research					594:601	the research	590:601	the research of kinetics and isotherms	590:627	In addition, the research of kinetics and isotherms displayed that the pseudo-second-order kinetic model and the Langmuir isotherm model fitted the data well.
32693272	6	20	theme	physical	805:812	arg1	adsorption					814:823	physical adsorption	805:823	physical adsorption	805:823	After further research, the different adsorption mechanism including physical adsorption, chemical adsorption and electrostatic interactions were discussed.
32693272	1	21	theme	hydrogel	180:187	arg1	composites					189:198	SA-PAM/GO hydrogel composites	170:198	SA-PAM/GO hydrogel composites	170:198	A novel bio-adsorbent named SA-PAM/GO hydrogel composites was synthesized through free radical polymerization.
32693272	7	22	theme	adsorption	983:992	arg1	adsorption					906:915	The chemical adsorption	893:915	The chemical adsorption accompanying the ion exchange process	893:953	The chemical adsorption accompanying the ion exchange process was confirmed as the staple adsorption mechanism.
32693272	7	22	theme	adsorption	983:992	arg1	mechanism					994:1002	the staple adsorption mechanism	972:1002	the staple adsorption mechanism	972:1002	The chemical adsorption accompanying the ion exchange process was confirmed as the staple adsorption mechanism.
32693272	3	23	theme	surface	374:380	arg1	area					382:385	the BET surface area	366:385	the BET surface area	366:385	After modification, the BET surface area increased more than tripled, which was consistent with SEM results.
32693272	0	24	theme	metal	131:135	arg1	ion					137:139	heavy metal ion	125:139	heavy metal ion	125:139	Preparation of a novel bio-adsorbent of sodium alginate grafted polyacrylamide/graphene oxide hydrogel for the adsorption of heavy metal ion.
32693272	6	25	theme	adsorption	774:783	arg1	mechanism					785:793	the different adsorption mechanism	760:793	the different adsorption mechanism including physical adsorption, chemical adsorption and electrostatic interactions	760:875	After further research, the different adsorption mechanism including physical adsorption, chemical adsorption and electrostatic interactions were discussed.
32693272	0	26	theme	heavy	125:129	arg1	ion					137:139	heavy metal ion	125:139	heavy metal ion	125:139	Preparation of a novel bio-adsorbent of sodium alginate grafted polyacrylamide/graphene oxide hydrogel for the adsorption of heavy metal ion.
32693272	9	27	theme	heavy	1191:1195	arg1	ions					1203:1206	the heavy metal ions	1187:1206	the heavy metal ions	1187:1206	Therefore, the SA-PAM/GO hydrogel composites have potential to remove the heavy metal ions from water body effectively.
32693272	1	28	theme	free	224:227	arg1	polymerization					237:250	free radical polymerization	224:250	free radical polymerization	224:250	A novel bio-adsorbent named SA-PAM/GO hydrogel composites was synthesized through free radical polymerization.
32693272	0	29	theme	bio-adsorbent	23:35	arg1	Preparation					0:10	Preparation	0:10	Preparation of a novel bio-adsorbent of sodium alginate	0:54	Preparation of a novel bio-adsorbent of sodium alginate grafted polyacrylamide/graphene oxide hydrogel for the adsorption of heavy metal ion.
32693272	4	30	theme	Pb2+	525:528	arg1	capacity					504:511	the maximum adsorption capacity	481:511	the maximum adsorption capacity of Cu2+ and Pb2+	481:528	Under optimal conditions, the maximum adsorption capacity of Cu2+ and Pb2+ were 68.76 mg/g and 240.69 mg/g, respectively.
32693272	4	30	theme	Pb2+	525:528	arg1	68.76 mg/g					535:544	68.76 mg/g	535:544	68.76 mg/g	535:544	Under optimal conditions, the maximum adsorption capacity of Cu2+ and Pb2+ were 68.76 mg/g and 240.69 mg/g, respectively.
32693272	0	31	theme	ion	137:139	arg1	adsorption					111:120	the adsorption	107:120	the adsorption of heavy metal ion	107:139	Preparation of a novel bio-adsorbent of sodium alginate grafted polyacrylamide/graphene oxide hydrogel for the adsorption of heavy metal ion.
32693272	4	32	theme	optimal	461:467	arg1	conditions					469:478	optimal conditions	461:478	optimal conditions	461:478	Under optimal conditions, the maximum adsorption capacity of Cu2+ and Pb2+ were 68.76 mg/g and 240.69 mg/g, respectively.
32693272	0	33	theme	novel	17:21	arg1	bio-adsorbent					23:35	a novel bio-adsorbent	15:35	a novel bio-adsorbent of sodium alginate	15:54	Preparation of a novel bio-adsorbent of sodium alginate grafted polyacrylamide/graphene oxide hydrogel for the adsorption of heavy metal ion.
32693272	6	34	theme	different	764:772	arg1	mechanism					785:793	the different adsorption mechanism	760:793	the different adsorption mechanism including physical adsorption, chemical adsorption and electrostatic interactions	760:875	After further research, the different adsorption mechanism including physical adsorption, chemical adsorption and electrostatic interactions were discussed.
32693272	1	35	theme	radical	229:235	arg1	polymerization					237:250	free radical polymerization	224:250	free radical polymerization	224:250	A novel bio-adsorbent named SA-PAM/GO hydrogel composites was synthesized through free radical polymerization.
32693272	0	36	theme	alginate	47:54	arg1	bio-adsorbent					23:35	a novel bio-adsorbent	15:35	a novel bio-adsorbent of sodium alginate	15:54	Preparation of a novel bio-adsorbent of sodium alginate grafted polyacrylamide/graphene oxide hydrogel for the adsorption of heavy metal ion.
32693272	0	37	theme	sodium	40:45	arg1	alginate					47:54	sodium alginate	40:54	sodium alginate	40:54	Preparation of a novel bio-adsorbent of sodium alginate grafted polyacrylamide/graphene oxide hydrogel for the adsorption of heavy metal ion.
32693272	3	38	with	consistent	426:435	arg1	results					446:452	SEM results	442:452	SEM results	442:452	After modification, the BET surface area increased more than tripled, which was consistent with SEM results.
32693272	5	39	theme	kinetic	668:674	arg1	model					676:680	the pseudo-second-order kinetic model	644:680	the pseudo-second-order kinetic model	644:680	In addition, the research of kinetics and isotherms displayed that the pseudo-second-order kinetic model and the Langmuir isotherm model fitted the data well.
32693272	5	40	theme	Langmuir	690:697	arg1	model					708:712	the Langmuir isotherm model	686:712	the Langmuir isotherm model	686:712	In addition, the research of kinetics and isotherms displayed that the pseudo-second-order kinetic model and the Langmuir isotherm model fitted the data well.
32693272	7	41	theme	staple	976:981	arg1	adsorption					906:915	The chemical adsorption	893:915	The chemical adsorption accompanying the ion exchange process	893:953	The chemical adsorption accompanying the ion exchange process was confirmed as the staple adsorption mechanism.
32693272	7	41	theme	staple	976:981	arg1	mechanism					994:1002	the staple adsorption mechanism	972:1002	the staple adsorption mechanism	972:1002	The chemical adsorption accompanying the ion exchange process was confirmed as the staple adsorption mechanism.
32693272	9	42	theme	water	1213:1217	arg1	body					1219:1222	water body	1213:1222	water body	1213:1222	Therefore, the SA-PAM/GO hydrogel composites have potential to remove the heavy metal ions from water body effectively.
32693272	5	43	theme	isotherm	699:706	arg1	model					708:712	the Langmuir isotherm model	686:712	the Langmuir isotherm model	686:712	In addition, the research of kinetics and isotherms displayed that the pseudo-second-order kinetic model and the Langmuir isotherm model fitted the data well.
32693272	6	44	theme	electrostatic	850:862	arg1	interactions					864:875	electrostatic interactions	850:875	electrostatic interactions	850:875	After further research, the different adsorption mechanism including physical adsorption, chemical adsorption and electrostatic interactions were discussed.
32693272	9	45	theme	metal	1197:1201	arg1	ions					1203:1206	the heavy metal ions	1187:1206	the heavy metal ions	1187:1206	Therefore, the SA-PAM/GO hydrogel composites have potential to remove the heavy metal ions from water body effectively.
32693272	6	46	theme	further	742:748	arg1	research					750:757	further research	742:757	further research	742:757	After further research, the different adsorption mechanism including physical adsorption, chemical adsorption and electrostatic interactions were discussed.
32693272	7	47	theme	exchange	938:945	arg1	process					947:953	the ion exchange process	930:953	the ion exchange process	930:953	The chemical adsorption accompanying the ion exchange process was confirmed as the staple adsorption mechanism.
32693272	0	48	theme	polyacrylamide/graphene	64:86	arg1	hydrogel					94:101	polyacrylamide/graphene oxide hydrogel	64:101	polyacrylamide/graphene oxide hydrogel for the adsorption of heavy metal ion	64:139	Preparation of a novel bio-adsorbent of sodium alginate grafted polyacrylamide/graphene oxide hydrogel for the adsorption of heavy metal ion.
32693272	3	49	theme	SEM	442:444	arg1	results					446:452	SEM results	442:452	SEM results	442:452	After modification, the BET surface area increased more than tripled, which was consistent with SEM results.
32693272	1	50	theme	novel	144:148	arg1	bio-adsorbent					150:162	A novel bio-adsorbent	142:162	A novel bio-adsorbent named SA-PAM/GO hydrogel composites	142:198	A novel bio-adsorbent named SA-PAM/GO hydrogel composites was synthesized through free radical polymerization.
33892424	0	0	theme	cellulose	92:100	arg1	conversion					68:77	green in-situ conversion	54:77	green in-situ conversion of bacterial cellulose for highly efficient removal of bisphenol A	54:144	Magnetic carbon nanofiber composite adsorbent through green in-situ conversion of bacterial cellulose for highly efficient removal of bisphenol A.
33892424	6	1	theme	magnetic	943:950	arg1	particles					952:960	magnetic particles	943:960	magnetic particles	943:960	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	1	2	theme	carbon	158:163	arg1	sorbent					175:181	A magnetic carbon nanofiber sorbent	147:181	A magnetic carbon nanofiber sorbent	147:181	A magnetic carbon nanofiber sorbent was facilely synthesized from bio-based bacterial cellulose and FeCl3via impregnation, freeze-drying, followed by pyrolysis at 700 °C, without additional activation or nanofiber fabrication.
33892424	0	3	theme	bacterial	82:90	arg1	cellulose					92:100	bacterial cellulose	82:100	bacterial cellulose	82:100	Magnetic carbon nanofiber composite adsorbent through green in-situ conversion of bacterial cellulose for highly efficient removal of bisphenol A.
33892424	6	4	with	adsorbent	1067:1075	arg1	characteristics					1094:1108	sustainable characteristics	1082:1108	sustainable characteristics	1082:1108	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	1	5	theme	nanofiber	165:173	arg1	sorbent					175:181	A magnetic carbon nanofiber sorbent	147:181	A magnetic carbon nanofiber sorbent	147:181	A magnetic carbon nanofiber sorbent was facilely synthesized from bio-based bacterial cellulose and FeCl3via impregnation, freeze-drying, followed by pyrolysis at 700 °C, without additional activation or nanofiber fabrication.
33892424	5	6	theme	magnetic	762:769	arg1	sorbent					771:777	The magnetic sorbent	758:777	The magnetic sorbent	758:777	The magnetic sorbent can maintain good magnetic properties even with recycling.
33892424	6	7	theme	nanofiber	898:906	arg1	precursor					908:916	a renewable carbon nanofiber precursor	879:916	a renewable carbon nanofiber precursor	879:916	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	6	8	theme	present	1004:1010	arg1	protocol					1012:1019	the present protocol	1000:1019	the present protocol	1000:1019	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	4	9	theme	initial	727:733	arg1	efficiency					746:755	initial adsorption efficiency	727:755	initial adsorption efficiency	727:755	Moreover, recycling the adsorbent for 10 consecutive cycles retained 96% of initial adsorption efficiency.
33892424	1	10	from	700 °C	310:315	arg1	pyrolysis					297:305	pyrolysis	297:305	pyrolysis at 700 °C	297:315	A magnetic carbon nanofiber sorbent was facilely synthesized from bio-based bacterial cellulose and FeCl3via impregnation, freeze-drying, followed by pyrolysis at 700 °C, without additional activation or nanofiber fabrication.
33892424	4	11	theme	consecutive	692:702	arg1	cycles					704:709	10 consecutive cycles	689:709	10 consecutive cycles	689:709	Moreover, recycling the adsorbent for 10 consecutive cycles retained 96% of initial adsorption efficiency.
33892424	0	12	theme	efficient	113:121	arg1	removal					123:129	highly efficient removal	106:129	highly efficient removal of bisphenol A	106:144	Magnetic carbon nanofiber composite adsorbent through green in-situ conversion of bacterial cellulose for highly efficient removal of bisphenol A.
33892424	2	13	contain	possessed	396:404	arg1	material					387:394	The obtained material	374:394	The obtained material	374:394	The obtained material possessed intrinsic 3D naturally fibrous and porous structure with good magnetization.
33892424	2	13	contain	possessed	396:404	arg2	structure					448:456	intrinsic 3D naturally fibrous and porous structure	406:456	intrinsic 3D naturally fibrous and porous structure	406:456	The obtained material possessed intrinsic 3D naturally fibrous and porous structure with good magnetization.
33892424	2	14	theme	fibrous	429:435	arg1	structure					448:456	intrinsic 3D naturally fibrous and porous structure	406:456	intrinsic 3D naturally fibrous and porous structure	406:456	The obtained material possessed intrinsic 3D naturally fibrous and porous structure with good magnetization.
33892424	0	15	theme	carbon	9:14	arg1	composite					26:34	Magnetic carbon nanofiber composite	0:34	Magnetic carbon nanofiber composite	0:34	Magnetic carbon nanofiber composite adsorbent through green in-situ conversion of bacterial cellulose for highly efficient removal of bisphenol A.
33892424	6	16	theme	sustainable	1082:1092	arg1	characteristics					1094:1108	sustainable characteristics	1082:1108	sustainable characteristics	1082:1108	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	0	17	theme	Magnetic	0:7	arg1	composite					26:34	Magnetic carbon nanofiber composite	0:34	Magnetic carbon nanofiber composite	0:34	Magnetic carbon nanofiber composite adsorbent through green in-situ conversion of bacterial cellulose for highly efficient removal of bisphenol A.
33892424	6	18	from	FeCl3	922:926	arg1	protocol					1012:1019	the present protocol	1000:1019	the present protocol	1000:1019	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	6	19	theme	bacterial	856:864	arg1	cellulose					866:874	bacterial cellulose	856:874	bacterial cellulose	856:874	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	2	20	theme	3D	416:417	arg1	structure					448:456	intrinsic 3D naturally fibrous and porous structure	406:456	intrinsic 3D naturally fibrous and porous structure	406:456	The obtained material possessed intrinsic 3D naturally fibrous and porous structure with good magnetization.
33892424	0	21	theme	bisphenol	134:142	arg1	A					144:144	bisphenol A	134:144	bisphenol A	134:144	Magnetic carbon nanofiber composite adsorbent through green in-situ conversion of bacterial cellulose for highly efficient removal of bisphenol A.
33892424	2	22	theme	good	463:466	arg1	magnetization					468:480	good magnetization	463:480	good magnetization	463:480	The obtained material possessed intrinsic 3D naturally fibrous and porous structure with good magnetization.
33892424	0	23	theme	nanofiber	16:24	arg1	composite					26:34	Magnetic carbon nanofiber composite	0:34	Magnetic carbon nanofiber composite	0:34	Magnetic carbon nanofiber composite adsorbent through green in-situ conversion of bacterial cellulose for highly efficient removal of bisphenol A.
33892424	6	24	theme	generating	980:989	arg1	agent					991:995	a green pore generating agent	967:995	a green pore generating agent in the present protocol	967:1019	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	2	25	theme	obtained	378:385	arg1	material					387:394	The obtained material	374:394	The obtained material	374:394	The obtained material possessed intrinsic 3D naturally fibrous and porous structure with good magnetization.
33892424	1	26	theme	bio-based	213:221	arg1	cellulose					233:241	bio-based bacterial cellulose	213:241	bio-based bacterial cellulose	213:241	A magnetic carbon nanofiber sorbent was facilely synthesized from bio-based bacterial cellulose and FeCl3via impregnation, freeze-drying, followed by pyrolysis at 700 °C, without additional activation or nanofiber fabrication.
33892424	6	27	from	agent	991:995	arg1	protocol					1012:1019	the present protocol	1000:1019	the present protocol	1000:1019	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	6	28	theme	pore	975:978	arg1	agent					991:995	a green pore generating agent	967:995	a green pore generating agent in the present protocol	967:1019	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	6	29	theme	carbon	1050:1055	arg1	nanofiber					1057:1065	a superior magnetic carbon nanofiber	1030:1065	a superior magnetic carbon nanofiber adsorbent with sustainable characteristics	1030:1108	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	6	30	from	use	849:851	arg1	protocol					1012:1019	the present protocol	1000:1019	the present protocol	1000:1019	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	1	31	theme	bacterial	223:231	arg1	cellulose					233:241	bio-based bacterial cellulose	213:241	bio-based bacterial cellulose	213:241	A magnetic carbon nanofiber sorbent was facilely synthesized from bio-based bacterial cellulose and FeCl3via impregnation, freeze-drying, followed by pyrolysis at 700 °C, without additional activation or nanofiber fabrication.
33892424	6	32	theme	green	969:973	arg1	agent					991:995	a green pore generating agent	967:995	a green pore generating agent in the present protocol	967:1019	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	0	33	theme	A	144:144	arg1	removal					123:129	highly efficient removal	106:129	highly efficient removal of bisphenol A	106:144	Magnetic carbon nanofiber composite adsorbent through green in-situ conversion of bacterial cellulose for highly efficient removal of bisphenol A.
33892424	6	34	theme	carbon	891:896	arg1	precursor					908:916	a renewable carbon nanofiber precursor	879:916	a renewable carbon nanofiber precursor	879:916	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	1	35	dep	synthesized	196:206	arg1	followed					285:292	followed	285:292	followed by pyrolysis at 700 °C	285:315	A magnetic carbon nanofiber sorbent was facilely synthesized from bio-based bacterial cellulose and FeCl3via impregnation, freeze-drying, followed by pyrolysis at 700 °C, without additional activation or nanofiber fabrication.
33892424	2	36	theme	porous	441:446	arg1	structure					448:456	intrinsic 3D naturally fibrous and porous structure	406:456	intrinsic 3D naturally fibrous and porous structure	406:456	The obtained material possessed intrinsic 3D naturally fibrous and porous structure with good magnetization.
33892424	0	37	theme	in-situ	60:66	arg1	conversion					68:77	green in-situ conversion	54:77	green in-situ conversion of bacterial cellulose for highly efficient removal of bisphenol A	54:144	Magnetic carbon nanofiber composite adsorbent through green in-situ conversion of bacterial cellulose for highly efficient removal of bisphenol A.
33892424	1	38	theme	additional	326:335	arg1	activation					337:346	additional activation	326:346	additional activation	326:346	A magnetic carbon nanofiber sorbent was facilely synthesized from bio-based bacterial cellulose and FeCl3via impregnation, freeze-drying, followed by pyrolysis at 700 °C, without additional activation or nanofiber fabrication.
33892424	6	39	theme	renewable	881:889	arg1	precursor					908:916	a renewable carbon nanofiber precursor	879:916	a renewable carbon nanofiber precursor	879:916	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	4	40	theme	efficiency	746:755	arg1	efficiency					746:755	initial adsorption efficiency	727:755	initial adsorption efficiency	727:755	Moreover, recycling the adsorbent for 10 consecutive cycles retained 96% of initial adsorption efficiency.
33892424	4	40	theme	efficiency	746:755	arg1	%					722:722	96%	720:722	96% of initial adsorption efficiency	720:755	Moreover, recycling the adsorbent for 10 consecutive cycles retained 96% of initial adsorption efficiency.
33892424	0	41	theme	green	54:58	arg1	conversion					68:77	green in-situ conversion	54:77	green in-situ conversion of bacterial cellulose for highly efficient removal of bisphenol A	54:144	Magnetic carbon nanofiber composite adsorbent through green in-situ conversion of bacterial cellulose for highly efficient removal of bisphenol A.
33892424	6	42	theme	particles	952:960	arg1	source					933:938	a source	931:938	a source of magnetic particles	931:960	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	3	43	theme	adsorbent	558:566	arg1	high					601:604	high	601:604	high	601:604	The adsorption results showed that the adsorption capacity of the prepared adsorbent towards bisphenol A (BPA) was as high as 618 mg/g, outperforming other adsorbents.
33892424	3	43	theme	adsorbent	558:566	arg1	capacity					533:540	the adsorption capacity	518:540	the adsorption capacity of the prepared adsorbent towards bisphenol A (BPA)	518:592	The adsorption results showed that the adsorption capacity of the prepared adsorbent towards bisphenol A (BPA) was as high as 618 mg/g, outperforming other adsorbents.
33892424	4	44	theme	adsorption	735:744	arg1	efficiency					746:755	initial adsorption efficiency	727:755	initial adsorption efficiency	727:755	Moreover, recycling the adsorbent for 10 consecutive cycles retained 96% of initial adsorption efficiency.
33892424	3	45	theme	other	633:637	arg1	adsorbents					639:648	other adsorbents	633:648	other adsorbents	633:648	The adsorption results showed that the adsorption capacity of the prepared adsorbent towards bisphenol A (BPA) was as high as 618 mg/g, outperforming other adsorbents.
33892424	3	46	theme	adsorption	522:531	arg1	high					601:604	high	601:604	high	601:604	The adsorption results showed that the adsorption capacity of the prepared adsorbent towards bisphenol A (BPA) was as high as 618 mg/g, outperforming other adsorbents.
33892424	3	46	theme	adsorption	522:531	arg1	capacity					533:540	the adsorption capacity	518:540	the adsorption capacity of the prepared adsorbent towards bisphenol A (BPA)	518:592	The adsorption results showed that the adsorption capacity of the prepared adsorbent towards bisphenol A (BPA) was as high as 618 mg/g, outperforming other adsorbents.
33892424	6	47	theme	superior	1032:1039	arg1	nanofiber					1057:1065	a superior magnetic carbon nanofiber	1030:1065	a superior magnetic carbon nanofiber adsorbent with sustainable characteristics	1030:1108	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	6	48	theme	cellulose	866:874	arg1	agent					991:995	a green pore generating agent	967:995	a green pore generating agent in the present protocol	967:1019	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	6	48	theme	cellulose	866:874	arg1	use					849:851	the use	845:851	the use of bacterial cellulose as a renewable carbon nanofiber precursor	845:916	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	6	48	theme	cellulose	866:874	arg1	FeCl3					922:926	FeCl3	922:926	FeCl3	922:926	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	3	49	theme	bisphenol	576:584	arg1	BPA					589:591	BPA	589:591	BPA	589:591	The adsorption results showed that the adsorption capacity of the prepared adsorbent towards bisphenol A (BPA) was as high as 618 mg/g, outperforming other adsorbents.
33892424	3	49	theme	bisphenol	576:584	arg1	A					586:586	bisphenol A	576:586	bisphenol A (BPA)	576:592	The adsorption results showed that the adsorption capacity of the prepared adsorbent towards bisphenol A (BPA) was as high as 618 mg/g, outperforming other adsorbents.
33892424	1	50	theme	FeCl3via	247:254	arg1	impregnation					256:267	FeCl3via impregnation	247:267	FeCl3via impregnation	247:267	A magnetic carbon nanofiber sorbent was facilely synthesized from bio-based bacterial cellulose and FeCl3via impregnation, freeze-drying, followed by pyrolysis at 700 °C, without additional activation or nanofiber fabrication.
33892424	5	51	theme	good	792:795	arg1	properties					806:815	good magnetic properties	792:815	good magnetic properties	792:815	The magnetic sorbent can maintain good magnetic properties even with recycling.
33892424	6	52	theme	magnetic	1041:1048	arg1	nanofiber					1057:1065	a superior magnetic carbon nanofiber	1030:1065	a superior magnetic carbon nanofiber adsorbent with sustainable characteristics	1030:1108	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	6	53	theme	adsorbent	1067:1075	arg1	nanofiber					1057:1065	a superior magnetic carbon nanofiber	1030:1065	a superior magnetic carbon nanofiber adsorbent with sustainable characteristics	1030:1108	Hence, the use of bacterial cellulose as a renewable carbon nanofiber precursor and FeCl3 as a source of magnetic particles, and a green pore generating agent in the present protocol, lead to a superior magnetic carbon nanofiber adsorbent with sustainable characteristics.
33892424	2	54	theme	intrinsic	406:414	arg1	structure					448:456	intrinsic 3D naturally fibrous and porous structure	406:456	intrinsic 3D naturally fibrous and porous structure	406:456	The obtained material possessed intrinsic 3D naturally fibrous and porous structure with good magnetization.
33892424	3	55	theme	adsorption	487:496	arg1	results					498:504	The adsorption results	483:504	The adsorption results	483:504	The adsorption results showed that the adsorption capacity of the prepared adsorbent towards bisphenol A (BPA) was as high as 618 mg/g, outperforming other adsorbents.
33892424	5	56	theme	magnetic	797:804	arg1	properties					806:815	good magnetic properties	792:815	good magnetic properties	792:815	The magnetic sorbent can maintain good magnetic properties even with recycling.
33892424	1	57	theme	nanofiber	351:359	arg1	fabrication					361:371	nanofiber fabrication	351:371	nanofiber fabrication	351:371	A magnetic carbon nanofiber sorbent was facilely synthesized from bio-based bacterial cellulose and FeCl3via impregnation, freeze-drying, followed by pyrolysis at 700 °C, without additional activation or nanofiber fabrication.
33892424	1	58	theme	magnetic	149:156	arg1	sorbent					175:181	A magnetic carbon nanofiber sorbent	147:181	A magnetic carbon nanofiber sorbent	147:181	A magnetic carbon nanofiber sorbent was facilely synthesized from bio-based bacterial cellulose and FeCl3via impregnation, freeze-drying, followed by pyrolysis at 700 °C, without additional activation or nanofiber fabrication.
34937335	12	0	theme	oral	1766:1769	arg1	delivery					1771:1778	oral delivery	1766:1778	oral delivery of protein-type drugs	1766:1800	All of these attributes indicate that the novel prepared hydrogel polymers have the potential as good carriers for oral delivery of protein-type drugs.
34937335	10	1	theme	model	1501:1505	arg1	applications					1411:1422	The drug delivery applications	1393:1422	The drug delivery applications of the prepared hydrogel	1393:1447	The drug delivery applications of the prepared hydrogel were evaluated with bovine serum albumin (BSA) as a model drug releasing in vitro.
34937335	10	1	theme	model	1501:1505	arg1	drug					1507:1510	a model drug	1499:1510	a model drug releasing in vitro	1499:1529	The drug delivery applications of the prepared hydrogel were evaluated with bovine serum albumin (BSA) as a model drug releasing in vitro.
34937335	3	2	from	group	492:496	arg1	residue					570:576	processed pineapple peel residue	545:576	processed pineapple peel residue	545:576	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	2	from	group	492:496	arg1	residue					649:655	processed HE residue	636:655	processed HE residue	636:655	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	7	3	theme	shortest	1153:1160	arg1	s					1178:1178	33 s	1175:1178	33 s	1175:1178	The gelation time experiments indicated that the shortest gel time was 33 s at a mass ratio of 4:6 (OHEC-70:CMCS).
34937335	7	3	theme	shortest	1153:1160	arg1	time					1166:1169	the shortest gel time	1149:1169	the shortest gel time	1149:1169	The gelation time experiments indicated that the shortest gel time was 33 s at a mass ratio of 4:6 (OHEC-70:CMCS).
34937335	1	4	theme	food	254:257	arg1	processing					259:268	food processing	254:268	food processing	254:268	Pineapple and hericium erinaceus (HE) produce a lot of residues in the process of food processing.
34937335	11	5	theme	simulated	1612:1620	arg1	conditions					1639:1648	simulated gastrointestinal conditions	1612:1648	simulated gastrointestinal conditions	1612:1648	It was discovered that the BSA release from the hydrogel was pH sensitive under simulated gastrointestinal conditions.
34937335	10	6	theme	delivery	1402:1409	arg1	applications					1411:1422	The drug delivery applications	1393:1422	The drug delivery applications of the prepared hydrogel	1393:1447	The drug delivery applications of the prepared hydrogel were evaluated with bovine serum albumin (BSA) as a model drug releasing in vitro.
34937335	10	6	theme	delivery	1402:1409	arg1	drug					1507:1510	a model drug	1499:1510	a model drug releasing in vitro	1499:1529	The drug delivery applications of the prepared hydrogel were evaluated with bovine serum albumin (BSA) as a model drug releasing in vitro.
34937335	0	7	theme	Smart	0:4	arg1	Hydrogel					19:26	Smart pH-Sensitive Hydrogel	0:26	Smart pH-Sensitive Hydrogel	0:26	Smart pH-Sensitive Hydrogel Based on the Pineapple Peel-Oxidized Hydroxyethyl Cellulose and the Hericium erinaceus Residue Carboxymethyl Chitosan for Use in Drug Delivery.
34937335	1	8	theme	hericium	186:193	arg1	erinaceus					195:203	hericium erinaceus	186:203	hericium erinaceus (HE)	186:208	Pineapple and hericium erinaceus (HE) produce a lot of residues in the process of food processing.
34937335	1	8	theme	hericium	186:193	arg1	HE					206:207	HE	206:207	HE	206:207	Pineapple and hericium erinaceus (HE) produce a lot of residues in the process of food processing.
34937335	1	9	theme	processing	259:268	arg1	process					243:249	the process	239:249	the process of food processing	239:268	Pineapple and hericium erinaceus (HE) produce a lot of residues in the process of food processing.
34937335	8	10	theme	swelling	1244:1251	arg1	properties					1253:1262	good swelling properties	1239:1262	good swelling properties	1239:1262	The hydrogel showed good swelling properties.
34937335	3	11	theme	peel	565:568	arg1	residue					570:576	processed pineapple peel residue	545:576	processed pineapple peel residue	545:576	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	11	12	from	hydrogel	1580:1587	arg1	sensitive					1596:1604	sensitive	1596:1604	sensitive	1596:1604	It was discovered that the BSA release from the hydrogel was pH sensitive under simulated gastrointestinal conditions.
34937335	11	12	from	hydrogel	1580:1587	arg1	release					1563:1569	the BSA release	1555:1569	the BSA release from the hydrogel	1555:1587	It was discovered that the BSA release from the hydrogel was pH sensitive under simulated gastrointestinal conditions.
34937335	6	13	theme	cellulose	1004:1012	arg1	adequate					1029:1036	adequate	1029:1036	adequate	1029:1036	It was observed that the modification of cellulose and chitin was adequate, and the synthesis of OHEC/CMCS hydrogel polymers was successful.
34937335	6	13	theme	cellulose	1004:1012	arg1	modification					988:999	the modification	984:999	the modification of cellulose and chitin	984:1023	It was observed that the modification of cellulose and chitin was adequate, and the synthesis of OHEC/CMCS hydrogel polymers was successful.
34937335	7	14	theme	gelation	1108:1115	arg1	experiments					1122:1132	The gelation time experiments	1104:1132	The gelation time experiments	1104:1132	The gelation time experiments indicated that the shortest gel time was 33 s at a mass ratio of 4:6 (OHEC-70:CMCS).
34937335	3	15	theme	processed	545:553	arg1	residue					570:576	processed pineapple peel residue	545:576	processed pineapple peel residue	545:576	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	16	theme	amino	586:590	arg1	OHEC					534:537	OHEC	534:537	OHEC	534:537	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	16	theme	amino	586:590	arg1	cellulose					523:531	oxidized hydroxyethyl cellulose	501:531	oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue	501:576	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	16	theme	amino	586:590	arg1	CMCS					625:628	CMCS	625:628	CMCS	625:628	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	16	theme	amino	586:590	arg1	group					592:596	the amino group	582:596	the amino group of carboxymethyl chitosan (CMCS) from processed HE residue	582:655	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	16	theme	amino	586:590	arg1	chitosan					615:622	carboxymethyl chitosan	601:622	carboxymethyl chitosan (CMCS) from processed HE residue	601:655	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	4	17	theme	experiments	684:694	arg1	series					674:679	a series	672:679	a series of experiments toward these new hydrogel polymers including structure characterization and performances	672:783	Subsequently, a series of experiments toward these new hydrogel polymers including structure characterization and performances were applied.
34937335	0	18	theme	Drug	157:160	arg1	Delivery					162:169	Drug Delivery	157:169	Drug Delivery	157:169	Smart pH-Sensitive Hydrogel Based on the Pineapple Peel-Oxidized Hydroxyethyl Cellulose and the Hericium erinaceus Residue Carboxymethyl Chitosan for Use in Drug Delivery.
34937335	3	19	theme	chitosan	615:622	arg1	CMCS					625:628	CMCS	625:628	CMCS	625:628	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	19	theme	chitosan	615:622	arg1	group					592:596	the amino group	582:596	the amino group of carboxymethyl chitosan (CMCS) from processed HE residue	582:655	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	19	theme	chitosan	615:622	arg1	cellulose					523:531	oxidized hydroxyethyl cellulose	501:531	oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue	501:576	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	19	theme	chitosan	615:622	arg1	chitosan					615:622	carboxymethyl chitosan	601:622	carboxymethyl chitosan (CMCS) from processed HE residue	601:655	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	19	theme	chitosan	615:622	arg1	OHEC					534:537	OHEC	534:537	OHEC	534:537	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	19	theme	chitosan	615:622	arg1	group					492:496	the aldehyde group	479:496	the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue	479:576	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	12	20	theme	novel	1693:1697	arg1	polymers					1717:1724	the novel prepared hydrogel polymers	1689:1724	the novel prepared hydrogel polymers	1689:1724	All of these attributes indicate that the novel prepared hydrogel polymers have the potential as good carriers for oral delivery of protein-type drugs.
34937335	0	21	theme	Hydroxyethyl	65:76	arg1	Cellulose					78:86	the Pineapple Peel-Oxidized Hydroxyethyl Cellulose	37:86	the Pineapple Peel-Oxidized Hydroxyethyl Cellulose	37:86	Smart pH-Sensitive Hydrogel Based on the Pineapple Peel-Oxidized Hydroxyethyl Cellulose and the Hericium erinaceus Residue Carboxymethyl Chitosan for Use in Drug Delivery.
34937335	9	22	theme	swelling	1277:1284	arg1	rate					1286:1289	The maximum swelling rate	1265:1289	The maximum swelling rate	1265:1289	The maximum swelling rate reached 11.58 g/g, and the swelling rate decreased with the increase of the oxidation degree of OHEC.
34937335	10	23	theme	prepared	1431:1438	arg1	hydrogel					1440:1447	the prepared hydrogel	1427:1447	the prepared hydrogel	1427:1447	The drug delivery applications of the prepared hydrogel were evaluated with bovine serum albumin (BSA) as a model drug releasing in vitro.
34937335	0	24	theme	Hericium	96:103	arg1	Chitosan					137:144	the Hericium erinaceus Residue Carboxymethyl Chitosan	92:144	the Hericium erinaceus Residue Carboxymethyl Chitosan for Use in Drug Delivery	92:169	Smart pH-Sensitive Hydrogel Based on the Pineapple Peel-Oxidized Hydroxyethyl Cellulose and the Hericium erinaceus Residue Carboxymethyl Chitosan for Use in Drug Delivery.
34937335	6	25	theme	chitin	1018:1023	arg1	adequate					1029:1036	adequate	1029:1036	adequate	1029:1036	It was observed that the modification of cellulose and chitin was adequate, and the synthesis of OHEC/CMCS hydrogel polymers was successful.
34937335	6	25	theme	chitin	1018:1023	arg1	modification					988:999	the modification	984:999	the modification of cellulose and chitin	984:1023	It was observed that the modification of cellulose and chitin was adequate, and the synthesis of OHEC/CMCS hydrogel polymers was successful.
34937335	3	26	theme	composite	424:432	arg1	hydrogel					434:441	one new composite hydrogel	416:441	one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue	416:655	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	0	27	theme	Residue	115:121	arg1	Chitosan					137:144	the Hericium erinaceus Residue Carboxymethyl Chitosan	92:144	the Hericium erinaceus Residue Carboxymethyl Chitosan for Use in Drug Delivery	92:169	Smart pH-Sensitive Hydrogel Based on the Pineapple Peel-Oxidized Hydroxyethyl Cellulose and the Hericium erinaceus Residue Carboxymethyl Chitosan for Use in Drug Delivery.
34937335	3	28	theme	non-toxic	374:382	arg1	method					384:389	a simple and non-toxic method	361:389	a simple and non-toxic method	361:389	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	29	theme	hydroxyethyl	510:521	arg1	OHEC					534:537	OHEC	534:537	OHEC	534:537	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	29	theme	hydroxyethyl	510:521	arg1	cellulose					523:531	oxidized hydroxyethyl cellulose	501:531	oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue	501:576	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	4	30	theme	hydrogel	713:720	arg1	polymers					722:729	these new hydrogel polymers	703:729	these new hydrogel polymers including structure characterization and performances	703:783	Subsequently, a series of experiments toward these new hydrogel polymers including structure characterization and performances were applied.
34937335	4	30	theme	hydrogel	713:720	arg1	characterization					751:766	structure characterization	741:766	structure characterization	741:766	Subsequently, a series of experiments toward these new hydrogel polymers including structure characterization and performances were applied.
34937335	4	30	theme	hydrogel	713:720	arg1	performances					772:783	performances	772:783	performances	772:783	Subsequently, a series of experiments toward these new hydrogel polymers including structure characterization and performances were applied.
34937335	5	31	theme	hydrogel	813:820	arg1	polymers					822:829	The resultant hydrogel polymers	799:829	The resultant hydrogel polymers	799:829	The resultant hydrogel polymers were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy and confirmed with thermogravimetry.
34937335	9	32	theme	swelling	1318:1325	arg1	rate					1327:1330	the swelling rate	1314:1330	the swelling rate	1314:1330	The maximum swelling rate reached 11.58 g/g, and the swelling rate decreased with the increase of the oxidation degree of OHEC.
34937335	3	33	theme	simple	363:368	arg1	method					384:389	a simple and non-toxic method	361:389	a simple and non-toxic method	361:389	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	34	theme	Schiff	450:455	arg1	reaction					462:469	the Schiff base reaction	446:469	the Schiff base reaction	446:469	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	7	35	theme	mass	1185:1188	arg1	ratio					1190:1194	a mass ratio	1183:1194	a mass ratio of 4:6 (OHEC-70:CMCS)	1183:1216	The gelation time experiments indicated that the shortest gel time was 33 s at a mass ratio of 4:6 (OHEC-70:CMCS).
34937335	6	36	theme	polymers	1079:1086	arg1	synthesis					1047:1055	the synthesis	1043:1055	the synthesis of OHEC/CMCS hydrogel polymers	1043:1086	It was observed that the modification of cellulose and chitin was adequate, and the synthesis of OHEC/CMCS hydrogel polymers was successful.
34937335	6	36	theme	polymers	1079:1086	arg1	successful					1092:1101	successful	1092:1101	successful	1092:1101	It was observed that the modification of cellulose and chitin was adequate, and the synthesis of OHEC/CMCS hydrogel polymers was successful.
34937335	10	37	theme	serum	1476:1480	arg1	albumin					1482:1488	bovine serum albumin	1469:1488	bovine serum albumin (BSA)	1469:1494	The drug delivery applications of the prepared hydrogel were evaluated with bovine serum albumin (BSA) as a model drug releasing in vitro.
34937335	10	37	theme	serum	1476:1480	arg1	BSA					1491:1493	BSA	1491:1493	BSA	1491:1493	The drug delivery applications of the prepared hydrogel were evaluated with bovine serum albumin (BSA) as a model drug releasing in vitro.
34937335	12	38	theme	protein-type	1783:1794	arg1	drugs					1796:1800	protein-type drugs	1783:1800	protein-type drugs	1783:1800	All of these attributes indicate that the novel prepared hydrogel polymers have the potential as good carriers for oral delivery of protein-type drugs.
34937335	6	39	theme	OHEC/CMCS	1060:1068	arg1	polymers					1079:1086	OHEC/CMCS hydrogel polymers	1060:1086	OHEC/CMCS hydrogel polymers	1060:1086	It was observed that the modification of cellulose and chitin was adequate, and the synthesis of OHEC/CMCS hydrogel polymers was successful.
34937335	11	40	theme	BSA	1559:1561	arg1	sensitive					1596:1604	sensitive	1596:1604	sensitive	1596:1604	It was discovered that the BSA release from the hydrogel was pH sensitive under simulated gastrointestinal conditions.
34937335	11	40	theme	BSA	1559:1561	arg1	release					1563:1569	the BSA release	1555:1569	the BSA release from the hydrogel	1555:1587	It was discovered that the BSA release from the hydrogel was pH sensitive under simulated gastrointestinal conditions.
34937335	0	41	theme	Pineapple	41:49	arg1	Cellulose					78:86	the Pineapple Peel-Oxidized Hydroxyethyl Cellulose	37:86	the Pineapple Peel-Oxidized Hydroxyethyl Cellulose	37:86	Smart pH-Sensitive Hydrogel Based on the Pineapple Peel-Oxidized Hydroxyethyl Cellulose and the Hericium erinaceus Residue Carboxymethyl Chitosan for Use in Drug Delivery.
34937335	7	42	dep	OHEC-70	1204:1210	arg1	CMCS					1212:1215	CMCS	1212:1215	CMCS	1212:1215	The gelation time experiments indicated that the shortest gel time was 33 s at a mass ratio of 4:6 (OHEC-70:CMCS).
34937335	5	43	dep	Fourier	853:859	arg1	transform					861:869	transform	861:869	transform infrared spectroscopy and scanning electron microscopy	861:924	The resultant hydrogel polymers were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy and confirmed with thermogravimetry.
34937335	9	44	theme	oxidation	1367:1375	arg1	degree					1377:1382	the oxidation degree	1363:1382	the oxidation degree of OHEC	1363:1390	The maximum swelling rate reached 11.58 g/g, and the swelling rate decreased with the increase of the oxidation degree of OHEC.
34937335	0	45	from	Use	150:152	arg1	Delivery					162:169	Drug Delivery	157:169	Drug Delivery	157:169	Smart pH-Sensitive Hydrogel Based on the Pineapple Peel-Oxidized Hydroxyethyl Cellulose and the Hericium erinaceus Residue Carboxymethyl Chitosan for Use in Drug Delivery.
34937335	3	46	theme	HE	646:647	arg1	residue					649:655	processed HE residue	636:655	processed HE residue	636:655	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	47	theme	oxidized	501:508	arg1	OHEC					534:537	OHEC	534:537	OHEC	534:537	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	47	theme	oxidized	501:508	arg1	cellulose					523:531	oxidized hydroxyethyl cellulose	501:531	oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue	501:576	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	1	48	theme	residues	227:234	arg1	residues					227:234	residues	227:234	residues	227:234	Pineapple and hericium erinaceus (HE) produce a lot of residues in the process of food processing.
34937335	1	48	theme	residues	227:234	arg1	lot					220:222	a lot	218:222	a lot of residues	218:234	Pineapple and hericium erinaceus (HE) produce a lot of residues in the process of food processing.
34937335	3	49	from	residue	649:655	arg1	CMCS					625:628	CMCS	625:628	CMCS	625:628	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	49	from	residue	649:655	arg1	group					592:596	the amino group	582:596	the amino group of carboxymethyl chitosan (CMCS) from processed HE residue	582:655	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	49	from	residue	649:655	arg1	cellulose					523:531	oxidized hydroxyethyl cellulose	501:531	oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue	501:576	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	49	from	residue	649:655	arg1	chitosan					615:622	carboxymethyl chitosan	601:622	carboxymethyl chitosan (CMCS) from processed HE residue	601:655	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	49	from	residue	649:655	arg1	OHEC					534:537	OHEC	534:537	OHEC	534:537	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	49	from	residue	649:655	arg1	group					492:496	the aldehyde group	479:496	the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue	479:576	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	7	50	theme	gel	1162:1164	arg1	s					1178:1178	33 s	1175:1178	33 s	1175:1178	The gelation time experiments indicated that the shortest gel time was 33 s at a mass ratio of 4:6 (OHEC-70:CMCS).
34937335	7	50	theme	gel	1162:1164	arg1	time					1166:1169	the shortest gel time	1149:1169	the shortest gel time	1149:1169	The gelation time experiments indicated that the shortest gel time was 33 s at a mass ratio of 4:6 (OHEC-70:CMCS).
34937335	12	51	theme	prepared	1699:1706	arg1	polymers					1717:1724	the novel prepared hydrogel polymers	1689:1724	the novel prepared hydrogel polymers	1689:1724	All of these attributes indicate that the novel prepared hydrogel polymers have the potential as good carriers for oral delivery of protein-type drugs.
34937335	2	52	theme	processed	277:285	arg1	precursors					326:335	good potential derivative precursors	300:335	good potential derivative precursors	300:335	These processed residues are good potential derivative precursors.
34937335	2	52	theme	processed	277:285	arg1	residues					287:294	These processed residues	271:294	These processed residues	271:294	These processed residues are good potential derivative precursors.
34937335	3	53	theme	pineapple	555:563	arg1	residue					570:576	processed pineapple peel residue	545:576	processed pineapple peel residue	545:576	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	12	54	theme	good	1748:1751	arg1	potential					1735:1743	the potential	1731:1743	the potential	1731:1743	All of these attributes indicate that the novel prepared hydrogel polymers have the potential as good carriers for oral delivery of protein-type drugs.
34937335	12	54	theme	good	1748:1751	arg1	carriers					1753:1760	good carriers	1748:1760	good carriers for oral delivery of protein-type drugs	1748:1800	All of these attributes indicate that the novel prepared hydrogel polymers have the potential as good carriers for oral delivery of protein-type drugs.
34937335	3	55	theme	cellulose	523:531	arg1	CMCS					625:628	CMCS	625:628	CMCS	625:628	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	55	theme	cellulose	523:531	arg1	group					592:596	the amino group	582:596	the amino group of carboxymethyl chitosan (CMCS) from processed HE residue	582:655	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	55	theme	cellulose	523:531	arg1	cellulose					523:531	oxidized hydroxyethyl cellulose	501:531	oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue	501:576	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	55	theme	cellulose	523:531	arg1	chitosan					615:622	carboxymethyl chitosan	601:622	carboxymethyl chitosan (CMCS) from processed HE residue	601:655	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	55	theme	cellulose	523:531	arg1	OHEC					534:537	OHEC	534:537	OHEC	534:537	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	55	theme	cellulose	523:531	arg1	group					492:496	the aldehyde group	479:496	the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue	479:576	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	12	56	theme	hydrogel	1708:1715	arg1	polymers					1717:1724	the novel prepared hydrogel polymers	1689:1724	the novel prepared hydrogel polymers	1689:1724	All of these attributes indicate that the novel prepared hydrogel polymers have the potential as good carriers for oral delivery of protein-type drugs.
34937335	11	57	theme	gastrointestinal	1622:1637	arg1	conditions					1639:1648	simulated gastrointestinal conditions	1612:1648	simulated gastrointestinal conditions	1612:1648	It was discovered that the BSA release from the hydrogel was pH sensitive under simulated gastrointestinal conditions.
34937335	7	58	theme	time	1117:1120	arg1	experiments					1122:1132	The gelation time experiments	1104:1132	The gelation time experiments	1104:1132	The gelation time experiments indicated that the shortest gel time was 33 s at a mass ratio of 4:6 (OHEC-70:CMCS).
34937335	10	59	theme	drug	1397:1400	arg1	applications					1411:1422	The drug delivery applications	1393:1422	The drug delivery applications of the prepared hydrogel	1393:1447	The drug delivery applications of the prepared hydrogel were evaluated with bovine serum albumin (BSA) as a model drug releasing in vitro.
34937335	10	59	theme	drug	1397:1400	arg1	drug					1507:1510	a model drug	1499:1510	a model drug releasing in vitro	1499:1529	The drug delivery applications of the prepared hydrogel were evaluated with bovine serum albumin (BSA) as a model drug releasing in vitro.
34937335	2	60	theme	potential	305:313	arg1	precursors					326:335	good potential derivative precursors	300:335	good potential derivative precursors	300:335	These processed residues are good potential derivative precursors.
34937335	2	60	theme	potential	305:313	arg1	residues					287:294	These processed residues	271:294	These processed residues	271:294	These processed residues are good potential derivative precursors.
34937335	8	61	theme	good	1239:1242	arg1	properties					1253:1262	good swelling properties	1239:1262	good swelling properties	1239:1262	The hydrogel showed good swelling properties.
34937335	2	62	theme	good	300:303	arg1	precursors					326:335	good potential derivative precursors	300:335	good potential derivative precursors	300:335	These processed residues are good potential derivative precursors.
34937335	2	62	theme	good	300:303	arg1	residues					287:294	These processed residues	271:294	These processed residues	271:294	These processed residues are good potential derivative precursors.
34937335	5	63	theme	scanning	897:904	arg1	microscopy					915:924	scanning electron microscopy	897:924	scanning electron microscopy	897:924	The resultant hydrogel polymers were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy and confirmed with thermogravimetry.
34937335	3	64	theme	carboxymethyl	601:613	arg1	CMCS					625:628	CMCS	625:628	CMCS	625:628	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	64	theme	carboxymethyl	601:613	arg1	chitosan					615:622	carboxymethyl chitosan	601:622	carboxymethyl chitosan (CMCS) from processed HE residue	601:655	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	12	65	contain	have	1726:1729	arg1	polymers					1717:1724	the novel prepared hydrogel polymers	1689:1724	the novel prepared hydrogel polymers	1689:1724	All of these attributes indicate that the novel prepared hydrogel polymers have the potential as good carriers for oral delivery of protein-type drugs.
34937335	12	65	contain	have	1726:1729	arg2	carriers					1753:1760	good carriers	1748:1760	good carriers for oral delivery of protein-type drugs	1748:1800	All of these attributes indicate that the novel prepared hydrogel polymers have the potential as good carriers for oral delivery of protein-type drugs.
34937335	12	65	contain	have	1726:1729	arg2	potential					1735:1743	the potential	1731:1743	the potential	1731:1743	All of these attributes indicate that the novel prepared hydrogel polymers have the potential as good carriers for oral delivery of protein-type drugs.
34937335	9	66	theme	maximum	1269:1275	arg1	rate					1286:1289	The maximum swelling rate	1265:1289	The maximum swelling rate	1265:1289	The maximum swelling rate reached 11.58 g/g, and the swelling rate decreased with the increase of the oxidation degree of OHEC.
34937335	5	67	theme	electron	906:913	arg1	microscopy					915:924	scanning electron microscopy	897:924	scanning electron microscopy	897:924	The resultant hydrogel polymers were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy and confirmed with thermogravimetry.
34937335	0	68	theme	erinaceus	105:113	arg1	Chitosan					137:144	the Hericium erinaceus Residue Carboxymethyl Chitosan	92:144	the Hericium erinaceus Residue Carboxymethyl Chitosan for Use in Drug Delivery	92:169	Smart pH-Sensitive Hydrogel Based on the Pineapple Peel-Oxidized Hydroxyethyl Cellulose and the Hericium erinaceus Residue Carboxymethyl Chitosan for Use in Drug Delivery.
34937335	4	69	theme	structure	741:749	arg1	characterization					751:766	structure characterization	741:766	structure characterization	741:766	Subsequently, a series of experiments toward these new hydrogel polymers including structure characterization and performances were applied.
34937335	3	70	from	group	592:596	arg1	residue					570:576	processed pineapple peel residue	545:576	processed pineapple peel residue	545:576	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	70	from	group	592:596	arg1	residue					649:655	processed HE residue	636:655	processed HE residue	636:655	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	0	71	theme	Carboxymethyl	123:135	arg1	Chitosan					137:144	the Hericium erinaceus Residue Carboxymethyl Chitosan	92:144	the Hericium erinaceus Residue Carboxymethyl Chitosan for Use in Drug Delivery	92:169	Smart pH-Sensitive Hydrogel Based on the Pineapple Peel-Oxidized Hydroxyethyl Cellulose and the Hericium erinaceus Residue Carboxymethyl Chitosan for Use in Drug Delivery.
34937335	7	72	from	ratio	1190:1194	arg1	s					1178:1178	33 s	1175:1178	33 s	1175:1178	The gelation time experiments indicated that the shortest gel time was 33 s at a mass ratio of 4:6 (OHEC-70:CMCS).
34937335	7	72	from	ratio	1190:1194	arg1	time					1166:1169	the shortest gel time	1149:1169	the shortest gel time	1149:1169	The gelation time experiments indicated that the shortest gel time was 33 s at a mass ratio of 4:6 (OHEC-70:CMCS).
34937335	7	73	theme	4:6	1199:1201	arg1	ratio					1190:1194	a mass ratio	1183:1194	a mass ratio of 4:6 (OHEC-70:CMCS)	1183:1216	The gelation time experiments indicated that the shortest gel time was 33 s at a mass ratio of 4:6 (OHEC-70:CMCS).
34937335	5	74	theme	resultant	803:811	arg1	polymers					822:829	The resultant hydrogel polymers	799:829	The resultant hydrogel polymers	799:829	The resultant hydrogel polymers were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy and confirmed with thermogravimetry.
34937335	10	75	theme	hydrogel	1440:1447	arg1	applications					1411:1422	The drug delivery applications	1393:1422	The drug delivery applications of the prepared hydrogel	1393:1447	The drug delivery applications of the prepared hydrogel were evaluated with bovine serum albumin (BSA) as a model drug releasing in vitro.
34937335	10	75	theme	hydrogel	1440:1447	arg1	drug					1507:1510	a model drug	1499:1510	a model drug releasing in vitro	1499:1529	The drug delivery applications of the prepared hydrogel were evaluated with bovine serum albumin (BSA) as a model drug releasing in vitro.
34937335	4	76	theme	new	709:711	arg1	polymers					722:729	these new hydrogel polymers	703:729	these new hydrogel polymers including structure characterization and performances	703:783	Subsequently, a series of experiments toward these new hydrogel polymers including structure characterization and performances were applied.
34937335	4	76	theme	new	709:711	arg1	characterization					751:766	structure characterization	741:766	structure characterization	741:766	Subsequently, a series of experiments toward these new hydrogel polymers including structure characterization and performances were applied.
34937335	4	76	theme	new	709:711	arg1	performances					772:783	performances	772:783	performances	772:783	Subsequently, a series of experiments toward these new hydrogel polymers including structure characterization and performances were applied.
34937335	3	77	from	residue	570:576	arg1	CMCS					625:628	CMCS	625:628	CMCS	625:628	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	77	from	residue	570:576	arg1	group					592:596	the amino group	582:596	the amino group of carboxymethyl chitosan (CMCS) from processed HE residue	582:655	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	77	from	residue	570:576	arg1	cellulose					523:531	oxidized hydroxyethyl cellulose	501:531	oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue	501:576	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	77	from	residue	570:576	arg1	chitosan					615:622	carboxymethyl chitosan	601:622	carboxymethyl chitosan (CMCS) from processed HE residue	601:655	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	77	from	residue	570:576	arg1	OHEC					534:537	OHEC	534:537	OHEC	534:537	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	77	from	residue	570:576	arg1	group					492:496	the aldehyde group	479:496	the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue	479:576	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	78	theme	new	420:422	arg1	hydrogel					434:441	one new composite hydrogel	416:441	one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue	416:655	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	79	theme	base	457:460	arg1	reaction					462:469	the Schiff base reaction	446:469	the Schiff base reaction	446:469	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	6	80	theme	hydrogel	1070:1077	arg1	polymers					1079:1086	OHEC/CMCS hydrogel polymers	1060:1086	OHEC/CMCS hydrogel polymers	1060:1086	It was observed that the modification of cellulose and chitin was adequate, and the synthesis of OHEC/CMCS hydrogel polymers was successful.
34937335	10	81	theme	bovine	1469:1474	arg1	albumin					1482:1488	bovine serum albumin	1469:1488	bovine serum albumin (BSA)	1469:1494	The drug delivery applications of the prepared hydrogel were evaluated with bovine serum albumin (BSA) as a model drug releasing in vitro.
34937335	10	81	theme	bovine	1469:1474	arg1	BSA					1491:1493	BSA	1491:1493	BSA	1491:1493	The drug delivery applications of the prepared hydrogel were evaluated with bovine serum albumin (BSA) as a model drug releasing in vitro.
34937335	0	82	theme	Peel-Oxidized	51:63	arg1	Cellulose					78:86	the Pineapple Peel-Oxidized Hydroxyethyl Cellulose	37:86	the Pineapple Peel-Oxidized Hydroxyethyl Cellulose	37:86	Smart pH-Sensitive Hydrogel Based on the Pineapple Peel-Oxidized Hydroxyethyl Cellulose and the Hericium erinaceus Residue Carboxymethyl Chitosan for Use in Drug Delivery.
34937335	3	83	theme	aldehyde	483:490	arg1	OHEC					534:537	OHEC	534:537	OHEC	534:537	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	83	theme	aldehyde	483:490	arg1	cellulose					523:531	oxidized hydroxyethyl cellulose	501:531	oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue	501:576	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	83	theme	aldehyde	483:490	arg1	CMCS					625:628	CMCS	625:628	CMCS	625:628	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	83	theme	aldehyde	483:490	arg1	group					492:496	the aldehyde group	479:496	the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue	479:576	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	3	83	theme	aldehyde	483:490	arg1	chitosan					615:622	carboxymethyl chitosan	601:622	carboxymethyl chitosan (CMCS) from processed HE residue	601:655	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	12	84	theme	drugs	1796:1800	arg1	delivery					1771:1778	oral delivery	1766:1778	oral delivery of protein-type drugs	1766:1800	All of these attributes indicate that the novel prepared hydrogel polymers have the potential as good carriers for oral delivery of protein-type drugs.
34937335	3	85	theme	processed	636:644	arg1	residue					649:655	processed HE residue	636:655	processed HE residue	636:655	In this investigation, a simple and non-toxic method was developed to prepare one new composite hydrogel by the Schiff base reaction between the aldehyde group of oxidized hydroxyethyl cellulose (OHEC) from processed pineapple peel residue and the amino group of carboxymethyl chitosan (CMCS) from processed HE residue.
34937335	5	86	dep	transform	861:869	arg1	infrared					871:878	infrared	871:878	transform infrared spectroscopy and scanning electron microscopy	861:924	The resultant hydrogel polymers were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy and confirmed with thermogravimetry.
34937335	2	87	theme	derivative	315:324	arg1	precursors					326:335	good potential derivative precursors	300:335	good potential derivative precursors	300:335	These processed residues are good potential derivative precursors.
34937335	2	87	theme	derivative	315:324	arg1	residues					287:294	These processed residues	271:294	These processed residues	271:294	These processed residues are good potential derivative precursors.
34937335	9	88	theme	degree	1377:1382	arg1	increase					1351:1358	the increase	1347:1358	the increase of the oxidation degree of OHEC	1347:1390	The maximum swelling rate reached 11.58 g/g, and the swelling rate decreased with the increase of the oxidation degree of OHEC.
34937335	0	89	theme	pH-Sensitive	6:17	arg1	Hydrogel					19:26	Smart pH-Sensitive Hydrogel	0:26	Smart pH-Sensitive Hydrogel	0:26	Smart pH-Sensitive Hydrogel Based on the Pineapple Peel-Oxidized Hydroxyethyl Cellulose and the Hericium erinaceus Residue Carboxymethyl Chitosan for Use in Drug Delivery.
34937335	9	90	theme	OHEC	1387:1390	arg1	degree					1377:1382	the oxidation degree	1363:1382	the oxidation degree of OHEC	1363:1390	The maximum swelling rate reached 11.58 g/g, and the swelling rate decreased with the increase of the oxidation degree of OHEC.
33910727	7	0	theme	fibroblast	1146:1155	arg1	functions					1166:1174	encapsulated fibroblast cellular functions	1133:1174	encapsulated fibroblast cellular functions	1133:1174	In addition, it was found that the developed bioink supported encapsulated fibroblast cellular functions.
33910727	2	1	theme	tissue	390:395	arg1	regeneration					397:408	tissue regeneration	390:408	tissue regeneration	390:408	However, the development of cell-laden bioink with potent antibacterial activity while supporting tissue regeneration proved to be challenging.
33910727	5	2	theme	hydrogel	781:788	arg1	bioink					790:795	the hydrogel bioink	777:795	the hydrogel bioink	777:795	First, Ga+3 stabilized the hydrogel bioink by the formation of ionic crosslinking with Alg chains.
33910727	4	3	with	formulation	721:731	arg1	functions					743:751	dual functions	738:751	dual functions	738:751	The key feature of the bioink is the use of gallium (Ga+3) in the design of bioink formulation with dual functions.
33910727	7	4	theme	cellular	1157:1164	arg1	functions					1166:1174	encapsulated fibroblast cellular functions	1133:1174	encapsulated fibroblast cellular functions	1133:1174	In addition, it was found that the developed bioink supported encapsulated fibroblast cellular functions.
33910727	1	5	theme	due	185:187	arg1	application					173:183	tissue engineering application	154:183	tissue engineering application due to the growing number of bacterial infections associated with impaired wound healing or bone implants	154:289	Bioink with inherent antibacterial activity is of particular interest for tissue engineering application due to the growing number of bacterial infections associated with impaired wound healing or bone implants.
33910727	1	6	theme	bone	277:280	arg1	implants					282:289	bone implants	277:289	bone implants	277:289	Bioink with inherent antibacterial activity is of particular interest for tissue engineering application due to the growing number of bacterial infections associated with impaired wound healing or bone implants.
33910727	3	7	theme	bacterial	617:625	arg1	infection					627:635	a bacterial infection	615:635	a bacterial infection	615:635	Here, we introduced a cell-laden antibacterial bioink based on Methylcellulose/Alginate (MC/Alg) hydrogel for skin tissue engineering via elimination of the risks associated with a bacterial infection.
33910727	4	8	theme	key	642:644	arg1	use					675:677	the use	671:677	the use of gallium (Ga+3) in the design of bioink formulation with dual functions	671:751	The key feature of the bioink is the use of gallium (Ga+3) in the design of bioink formulation with dual functions.
33910727	4	8	theme	key	642:644	arg1	feature					646:652	The key feature	638:652	The key feature of the bioink	638:666	The key feature of the bioink is the use of gallium (Ga+3) in the design of bioink formulation with dual functions.
33910727	5	9	with	crosslinking	823:834	arg1	chains					845:850	Alg chains	841:850	Alg chains	841:850	First, Ga+3 stabilized the hydrogel bioink by the formation of ionic crosslinking with Alg chains.
33910727	3	10	theme	risks	593:597	arg1	elimination					574:584	elimination	574:584	elimination of the risks associated with a bacterial infection	574:635	Here, we introduced a cell-laden antibacterial bioink based on Methylcellulose/Alginate (MC/Alg) hydrogel for skin tissue engineering via elimination of the risks associated with a bacterial infection.
33910727	3	11	theme	skin	546:549	arg1	engineering					558:568	skin tissue engineering	546:568	skin tissue engineering via elimination of the risks associated with a bacterial infection	546:635	Here, we introduced a cell-laden antibacterial bioink based on Methylcellulose/Alginate (MC/Alg) hydrogel for skin tissue engineering via elimination of the risks associated with a bacterial infection.
33910727	2	12	with	bioink	331:336	arg1	activity					364:371	potent antibacterial activity	343:371	potent antibacterial activity while supporting tissue regeneration	343:408	However, the development of cell-laden bioink with potent antibacterial activity while supporting tissue regeneration proved to be challenging.
33910727	6	13	theme	antibacterial	909:921	arg1	activity					923:930	potent antibacterial activity	902:930	potent antibacterial activity toward both Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria	902:1032	Second, the gallium-crosslinked bioink exhibited potent antibacterial activity toward both Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria with a bactericidal rate of 99.99 %.
33910727	7	14	theme	developed	1106:1114	arg1	bioink					1116:1121	the developed bioink	1102:1121	the developed bioink	1102:1121	In addition, it was found that the developed bioink supported encapsulated fibroblast cellular functions.
33910727	3	15	theme	antibacterial	469:481	arg1	bioink					483:488	a cell-laden antibacterial bioink	456:488	a cell-laden antibacterial bioink based on Methylcellulose/Alginate (MC/Alg) hydrogel for skin tissue engineering via elimination of the risks associated with a bacterial infection	456:635	Here, we introduced a cell-laden antibacterial bioink based on Methylcellulose/Alginate (MC/Alg) hydrogel for skin tissue engineering via elimination of the risks associated with a bacterial infection.
33910727	6	16	dep	Gram-positive	944:956	arg1	bacteria					1025:1032	bacteria	1025:1032	bacteria	1025:1032	Second, the gallium-crosslinked bioink exhibited potent antibacterial activity toward both Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria with a bactericidal rate of 99.99 %.
33910727	6	16	dep	Gram-positive	944:956	arg1	aureus					974:979	Staphylococcus aureus	959:979	Staphylococcus aureus	959:979	Second, the gallium-crosslinked bioink exhibited potent antibacterial activity toward both Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria with a bactericidal rate of 99.99 %.
33910727	6	17	theme	potent	902:907	arg1	activity					923:930	potent antibacterial activity	902:930	potent antibacterial activity toward both Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria	902:1032	Second, the gallium-crosslinked bioink exhibited potent antibacterial activity toward both Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria with a bactericidal rate of 99.99 %.
33910727	1	18	with	Bioink	80:85	arg1	activity					115:122	inherent antibacterial activity	92:122	inherent antibacterial activity	92:122	Bioink with inherent antibacterial activity is of particular interest for tissue engineering application due to the growing number of bacterial infections associated with impaired wound healing or bone implants.
33910727	5	19	theme	ionic	817:821	arg1	crosslinking					823:834	ionic crosslinking	817:834	ionic crosslinking with Alg chains	817:850	First, Ga+3 stabilized the hydrogel bioink by the formation of ionic crosslinking with Alg chains.
33910727	4	20	theme	gallium	682:688	arg1	use					675:677	the use	671:677	the use of gallium (Ga+3) in the design of bioink formulation with dual functions	671:751	The key feature of the bioink is the use of gallium (Ga+3) in the design of bioink formulation with dual functions.
33910727	4	20	theme	gallium	682:688	arg1	feature					646:652	The key feature	638:652	The key feature of the bioink	638:666	The key feature of the bioink is the use of gallium (Ga+3) in the design of bioink formulation with dual functions.
33910727	1	21	theme	inherent	92:99	arg1	activity					115:122	inherent antibacterial activity	92:122	inherent antibacterial activity	92:122	Bioink with inherent antibacterial activity is of particular interest for tissue engineering application due to the growing number of bacterial infections associated with impaired wound healing or bone implants.
33910727	1	22	theme	growing	196:202	arg1	number					204:209	the growing number	192:209	the growing number of bacterial infections associated with impaired wound healing or bone implants	192:289	Bioink with inherent antibacterial activity is of particular interest for tissue engineering application due to the growing number of bacterial infections associated with impaired wound healing or bone implants.
33910727	0	23	theme	3D	0:1	arg1	bioprinting					3:13	3D bioprinting	0:13	3D bioprinting of a cell-laden antibacterial polysaccharide hydrogel composite	0:77	3D bioprinting of a cell-laden antibacterial polysaccharide hydrogel composite.
33910727	1	24	theme	antibacterial	101:113	arg1	activity					115:122	inherent antibacterial activity	92:122	inherent antibacterial activity	92:122	Bioink with inherent antibacterial activity is of particular interest for tissue engineering application due to the growing number of bacterial infections associated with impaired wound healing or bone implants.
33910727	7	25	located	found	1091:1095	arg2	it					1084:1085	it	1084:1085	it	1084:1085	In addition, it was found that the developed bioink supported encapsulated fibroblast cellular functions.
33910727	7	25	located	found	1091:1095	arg1	addition					1074:1081	addition	1074:1081	addition	1074:1081	In addition, it was found that the developed bioink supported encapsulated fibroblast cellular functions.
33910727	3	26	theme	cell-laden	458:467	arg1	bioink					483:488	a cell-laden antibacterial bioink	456:488	a cell-laden antibacterial bioink based on Methylcellulose/Alginate (MC/Alg) hydrogel for skin tissue engineering via elimination of the risks associated with a bacterial infection	456:635	Here, we introduced a cell-laden antibacterial bioink based on Methylcellulose/Alginate (MC/Alg) hydrogel for skin tissue engineering via elimination of the risks associated with a bacterial infection.
33910727	5	27	theme	crosslinking	823:834	arg1	formation					804:812	the formation	800:812	the formation of ionic crosslinking with Alg chains	800:850	First, Ga+3 stabilized the hydrogel bioink by the formation of ionic crosslinking with Alg chains.
33910727	7	28	theme	encapsulated	1133:1144	arg1	functions					1166:1174	encapsulated fibroblast cellular functions	1133:1174	encapsulated fibroblast cellular functions	1133:1174	In addition, it was found that the developed bioink supported encapsulated fibroblast cellular functions.
33910727	2	29	theme	cell-laden	320:329	arg1	bioink					331:336	cell-laden bioink	320:336	cell-laden bioink with potent antibacterial activity while supporting tissue regeneration	320:408	However, the development of cell-laden bioink with potent antibacterial activity while supporting tissue regeneration proved to be challenging.
33910727	4	30	from	use	675:677	arg1	design					704:709	the design	700:709	the design of bioink formulation with dual functions	700:751	The key feature of the bioink is the use of gallium (Ga+3) in the design of bioink formulation with dual functions.
33910727	3	31	theme	Methylcellulose/Alginate	499:522	arg1	hydrogel					533:540	Methylcellulose/Alginate (MC/Alg) hydrogel	499:540	Methylcellulose/Alginate (MC/Alg) hydrogel for skin tissue engineering via elimination of the risks associated with a bacterial infection	499:635	Here, we introduced a cell-laden antibacterial bioink based on Methylcellulose/Alginate (MC/Alg) hydrogel for skin tissue engineering via elimination of the risks associated with a bacterial infection.
33910727	2	32	theme	bioink	331:336	arg1	challenging					423:433	challenging	423:433	challenging	423:433	However, the development of cell-laden bioink with potent antibacterial activity while supporting tissue regeneration proved to be challenging.
33910727	2	32	theme	bioink	331:336	arg1	development					305:315	the development	301:315	the development of cell-laden bioink with potent antibacterial activity while supporting tissue regeneration	301:408	However, the development of cell-laden bioink with potent antibacterial activity while supporting tissue regeneration proved to be challenging.
33910727	3	33	theme	tissue	551:556	arg1	engineering					558:568	skin tissue engineering	546:568	skin tissue engineering via elimination of the risks associated with a bacterial infection	546:635	Here, we introduced a cell-laden antibacterial bioink based on Methylcellulose/Alginate (MC/Alg) hydrogel for skin tissue engineering via elimination of the risks associated with a bacterial infection.
33910727	1	34	theme	bacterial	214:222	arg1	infections					224:233	bacterial infections	214:233	bacterial infections associated with impaired wound healing or bone implants	214:289	Bioink with inherent antibacterial activity is of particular interest for tissue engineering application due to the growing number of bacterial infections associated with impaired wound healing or bone implants.
33910727	0	35	theme	antibacterial	31:43	arg1	composite					69:77	a cell-laden antibacterial polysaccharide hydrogel composite	18:77	a cell-laden antibacterial polysaccharide hydrogel composite	18:77	3D bioprinting of a cell-laden antibacterial polysaccharide hydrogel composite.
33910727	4	36	theme	bioink	714:719	arg1	formulation					721:731	bioink formulation	714:731	bioink formulation with dual functions	714:751	The key feature of the bioink is the use of gallium (Ga+3) in the design of bioink formulation with dual functions.
33910727	6	37	theme	gallium-crosslinked	865:883	arg1	bioink					885:890	the gallium-crosslinked bioink	861:890	the gallium-crosslinked bioink	861:890	Second, the gallium-crosslinked bioink exhibited potent antibacterial activity toward both Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria with a bactericidal rate of 99.99 %.
33910727	1	38	theme	infections	224:233	arg1	number					204:209	the growing number	192:209	the growing number of bacterial infections associated with impaired wound healing or bone implants	192:289	Bioink with inherent antibacterial activity is of particular interest for tissue engineering application due to the growing number of bacterial infections associated with impaired wound healing or bone implants.
33910727	0	39	theme	cell-laden	20:29	arg1	composite					69:77	a cell-laden antibacterial polysaccharide hydrogel composite	18:77	a cell-laden antibacterial polysaccharide hydrogel composite	18:77	3D bioprinting of a cell-laden antibacterial polysaccharide hydrogel composite.
33910727	4	40	theme	bioink	661:666	arg1	use					675:677	the use	671:677	the use of gallium (Ga+3) in the design of bioink formulation with dual functions	671:751	The key feature of the bioink is the use of gallium (Ga+3) in the design of bioink formulation with dual functions.
33910727	4	40	theme	bioink	661:666	arg1	feature					646:652	The key feature	638:652	The key feature of the bioink	638:666	The key feature of the bioink is the use of gallium (Ga+3) in the design of bioink formulation with dual functions.
33910727	6	41	theme	%	1068:1068	arg1	rate					1054:1057	a bactericidal rate	1039:1057	a bactericidal rate of 99.99 %	1039:1068	Second, the gallium-crosslinked bioink exhibited potent antibacterial activity toward both Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria with a bactericidal rate of 99.99 %.
33910727	0	42	theme	hydrogel	60:67	arg1	composite					69:77	a cell-laden antibacterial polysaccharide hydrogel composite	18:77	a cell-laden antibacterial polysaccharide hydrogel composite	18:77	3D bioprinting of a cell-laden antibacterial polysaccharide hydrogel composite.
33910727	4	43	theme	formulation	721:731	arg1	design					704:709	the design	700:709	the design of bioink formulation with dual functions	700:751	The key feature of the bioink is the use of gallium (Ga+3) in the design of bioink formulation with dual functions.
33910727	0	44	theme	polysaccharide	45:58	arg1	composite					69:77	a cell-laden antibacterial polysaccharide hydrogel composite	18:77	a cell-laden antibacterial polysaccharide hydrogel composite	18:77	3D bioprinting of a cell-laden antibacterial polysaccharide hydrogel composite.
33910727	3	45	theme	MC/Alg	525:530	arg1	hydrogel					533:540	Methylcellulose/Alginate (MC/Alg) hydrogel	499:540	Methylcellulose/Alginate (MC/Alg) hydrogel for skin tissue engineering via elimination of the risks associated with a bacterial infection	499:635	Here, we introduced a cell-laden antibacterial bioink based on Methylcellulose/Alginate (MC/Alg) hydrogel for skin tissue engineering via elimination of the risks associated with a bacterial infection.
33910727	1	46	theme	particular	130:139	arg1	interest					141:148	particular interest	130:148	particular interest	130:148	Bioink with inherent antibacterial activity is of particular interest for tissue engineering application due to the growing number of bacterial infections associated with impaired wound healing or bone implants.
33910727	6	47	dep	Gram-negative	986:998	arg1	aeruginosa					1013:1022	Pseudomonas aeruginosa	1001:1022	Pseudomonas aeruginosa	1001:1022	Second, the gallium-crosslinked bioink exhibited potent antibacterial activity toward both Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria with a bactericidal rate of 99.99 %.
33910727	2	48	theme	antibacterial	350:362	arg1	activity					364:371	potent antibacterial activity	343:371	potent antibacterial activity while supporting tissue regeneration	343:408	However, the development of cell-laden bioink with potent antibacterial activity while supporting tissue regeneration proved to be challenging.
33910727	4	49	theme	dual	738:741	arg1	functions					743:751	dual functions	738:751	dual functions	738:751	The key feature of the bioink is the use of gallium (Ga+3) in the design of bioink formulation with dual functions.
33910727	2	50	theme	potent	343:348	arg1	activity					364:371	potent antibacterial activity	343:371	potent antibacterial activity while supporting tissue regeneration	343:408	However, the development of cell-laden bioink with potent antibacterial activity while supporting tissue regeneration proved to be challenging.
33910727	1	51	theme	impaired	251:258	arg1	healing					266:272	impaired wound healing	251:272	impaired wound healing	251:272	Bioink with inherent antibacterial activity is of particular interest for tissue engineering application due to the growing number of bacterial infections associated with impaired wound healing or bone implants.
33910727	5	52	theme	Alg	841:843	arg1	chains					845:850	Alg chains	841:850	Alg chains	841:850	First, Ga+3 stabilized the hydrogel bioink by the formation of ionic crosslinking with Alg chains.
33910727	1	53	theme	tissue	154:159	arg1	engineering					161:171	tissue engineering	154:171	tissue engineering application due to the growing number of bacterial infections associated with impaired wound healing or bone implants	154:289	Bioink with inherent antibacterial activity is of particular interest for tissue engineering application due to the growing number of bacterial infections associated with impaired wound healing or bone implants.
33910727	1	54	theme	wound	260:264	arg1	healing					266:272	impaired wound healing	251:272	impaired wound healing	251:272	Bioink with inherent antibacterial activity is of particular interest for tissue engineering application due to the growing number of bacterial infections associated with impaired wound healing or bone implants.
33910727	0	55	theme	composite	69:77	arg1	bioprinting					3:13	3D bioprinting	0:13	3D bioprinting of a cell-laden antibacterial polysaccharide hydrogel composite	0:77	3D bioprinting of a cell-laden antibacterial polysaccharide hydrogel composite.
33910727	6	56	link	gallium-crosslinked	865:883	arg1	bioink					885:890	the gallium-crosslinked bioink	861:890	the gallium-crosslinked bioink	861:890	Second, the gallium-crosslinked bioink exhibited potent antibacterial activity toward both Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria with a bactericidal rate of 99.99 %.
33910727	1	57	theme	engineering	161:171	arg1	application					173:183	tissue engineering application	154:183	tissue engineering application due to the growing number of bacterial infections associated with impaired wound healing or bone implants	154:289	Bioink with inherent antibacterial activity is of particular interest for tissue engineering application due to the growing number of bacterial infections associated with impaired wound healing or bone implants.
33910727	6	58	theme	bactericidal	1041:1052	arg1	rate					1054:1057	a bactericidal rate	1039:1057	a bactericidal rate of 99.99 %	1039:1068	Second, the gallium-crosslinked bioink exhibited potent antibacterial activity toward both Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria with a bactericidal rate of 99.99 %.
32522547	3	0	theme	time	266:269	arg1	effect					241:246	the effect	237:246	the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution	237:398	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	0	1	from	acid-extraction	16:30	arg1	grape					56:60	grape	56:60	grape (Vitis vinifera cv	56:79	Optimization of acid-extraction of pectic fraction from grape (Vitis vinifera cv.
32522547	3	2	theme	composite	189:197	arg1	design					199:204	A central composite design	179:204	A central composite design	179:204	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	3	3	theme	HNO3	386:389	arg1	solution					391:398	boiling HNO3 solution	378:398	boiling HNO3 solution	378:398	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	9	4	contain	had	1049:1051	arg1	It					970:971	It	970:971	It	970:971	It contained a low-methoxyl pectin (degree of methyl-esterification 18.1%) and had an average molar mass of 154,100 g/mol.
32522547	9	4	contain	had	1049:1051	arg2	mass					1070:1073	an average molar mass	1053:1073	an average molar mass of 154,100 g/mol	1053:1090	It contained a low-methoxyl pectin (degree of methyl-esterification 18.1%) and had an average molar mass of 154,100 g/mol.
32522547	8	5	theme	minor	922:926	arg1	amounts					928:934	minor amounts	922:934	minor amounts	922:934	The starch-free pectic fraction was composed of 63.5% UA, 7.8% rhamnose, 6.0% arabinose, 13.6% galactose and minor amounts of other neutral monosaccharides.
32522547	8	5	theme	minor	922:926	arg1	monosaccharides					953:967	other neutral monosaccharides	939:967	other neutral monosaccharides	939:967	The starch-free pectic fraction was composed of 63.5% UA, 7.8% rhamnose, 6.0% arabinose, 13.6% galactose and minor amounts of other neutral monosaccharides.
32522547	10	6	theme	%	1113:1113	arg1	homogalacturonan					1115:1130	55.7% homogalacturonan	1109:1130	55.7% homogalacturonan	1109:1130	It consisted of 55.7% homogalacturonan and 35.2% rhamnogalacturonan I (RG-I).
32522547	9	7	theme	low-methoxyl	985:996	arg1	pectin					998:1003	a low-methoxyl pectin	983:1003	a low-methoxyl pectin (degree of methyl-esterification 18.1%)	983:1043	It contained a low-methoxyl pectin (degree of methyl-esterification 18.1%) and had an average molar mass of 154,100 g/mol.
32522547	11	8	theme	single	1258:1263	arg1	residues					1265:1272	single residues	1258:1272	single residues	1258:1272	NMR analyses suggest that RG-I portion of α-GPOP is highly branched by short chains or single residues of arabinose and galactose.
32522547	11	8	theme	single	1258:1263	arg1	galactose					1291:1299	galactose	1291:1299	galactose	1291:1299	NMR analyses suggest that RG-I portion of α-GPOP is highly branched by short chains or single residues of arabinose and galactose.
32522547	11	8	theme	single	1258:1263	arg1	arabinose					1277:1285	arabinose	1277:1285	arabinose	1277:1285	NMR analyses suggest that RG-I portion of α-GPOP is highly branched by short chains or single residues of arabinose and galactose.
32522547	3	9	used	used	210:213	arg2	design					199:204	A central composite design	179:204	A central composite design	179:204	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	8	10	theme	starch-free	817:827	arg1	fraction					836:843	The starch-free pectic fraction	813:843	The starch-free pectic fraction	813:843	The starch-free pectic fraction was composed of 63.5% UA, 7.8% rhamnose, 6.0% arabinose, 13.6% galactose and minor amounts of other neutral monosaccharides.
32522547	9	11	dep	pectin	998:1003	arg1	degree					1006:1011	degree	1006:1011	degree of methyl-esterification 18.1%	1006:1042	It contained a low-methoxyl pectin (degree of methyl-esterification 18.1%) and had an average molar mass of 154,100 g/mol.
32522547	0	12	from	grape	56:60	arg1	acid-extraction					16:30	acid-extraction	16:30	acid-extraction of pectic fraction from grape (Vitis vinifera cv	16:79	Optimization of acid-extraction of pectic fraction from grape (Vitis vinifera cv.
32522547	0	12	from	grape	56:60	arg1	fraction					42:49	pectic fraction	35:49	pectic fraction from grape (Vitis vinifera cv	35:79	Optimization of acid-extraction of pectic fraction from grape (Vitis vinifera cv.
32522547	3	13	theme	central	181:187	arg1	design					199:204	A central composite design	179:204	A central composite design	179:204	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	8	14	dep	%	874:874	arg1	rhamnose					876:883	rhamnose	876:883	7.8% rhamnose	871:883	The starch-free pectic fraction was composed of 63.5% UA, 7.8% rhamnose, 6.0% arabinose, 13.6% galactose and minor amounts of other neutral monosaccharides.
32522547	3	15	from	effect	241:246	arg1	content					339:345	uronic acid (UA) content	322:345	uronic acid (UA) content	322:345	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	3	15	from	effect	241:246	arg1	yield					312:316	yield	312:316	yield	312:316	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	8	16	theme	monosaccharides	953:967	arg1	%					906:906	13.6% galactose	902:916	13.6% galactose	902:916	The starch-free pectic fraction was composed of 63.5% UA, 7.8% rhamnose, 6.0% arabinose, 13.6% galactose and minor amounts of other neutral monosaccharides.
32522547	8	16	theme	monosaccharides	953:967	arg1	%					865:865	63.5% UA	861:868	63.5% UA	861:868	The starch-free pectic fraction was composed of 63.5% UA, 7.8% rhamnose, 6.0% arabinose, 13.6% galactose and minor amounts of other neutral monosaccharides.
32522547	8	16	theme	monosaccharides	953:967	arg1	monosaccharides					953:967	other neutral monosaccharides	939:967	other neutral monosaccharides	939:967	The starch-free pectic fraction was composed of 63.5% UA, 7.8% rhamnose, 6.0% arabinose, 13.6% galactose and minor amounts of other neutral monosaccharides.
32522547	8	16	theme	monosaccharides	953:967	arg1	%					889:889	6.0% arabinose	886:899	6.0% arabinose	886:899	The starch-free pectic fraction was composed of 63.5% UA, 7.8% rhamnose, 6.0% arabinose, 13.6% galactose and minor amounts of other neutral monosaccharides.
32522547	8	16	theme	monosaccharides	953:967	arg1	amounts					928:934	minor amounts	922:934	minor amounts	922:934	The starch-free pectic fraction was composed of 63.5% UA, 7.8% rhamnose, 6.0% arabinose, 13.6% galactose and minor amounts of other neutral monosaccharides.
32522547	8	16	theme	monosaccharides	953:967	arg1	%					874:874	7.8%	871:874	7.8% rhamnose	871:883	The starch-free pectic fraction was composed of 63.5% UA, 7.8% rhamnose, 6.0% arabinose, 13.6% galactose and minor amounts of other neutral monosaccharides.
32522547	2	17	theme	grape	129:133	arg1	source					161:166	a source	159:166	a source of pectin	159:176	Chardonnay grape pomace was evaluated as a source of pectin.
32522547	2	17	theme	grape	129:133	arg1	pomace					135:140	Chardonnay grape pomace	118:140	Chardonnay grape pomace	118:140	Chardonnay grape pomace was evaluated as a source of pectin.
32522547	9	18	dep	degree	1006:1011	arg1	%					1042:1042	18.1%	1038:1042	degree of methyl-esterification 18.1%	1006:1042	It contained a low-methoxyl pectin (degree of methyl-esterification 18.1%) and had an average molar mass of 154,100 g/mol.
32522547	4	19	theme	%	560:560	arg1	yield					547:551	theoretical yield	535:551	theoretical yield of 12.8% and UA of 64.4%	535:576	The optimized extraction condition to reach the maximum yield and UA was pH = 2.08, Et = 135.23 min and LS = 35.11 ml/g, resulting in theoretical yield of 12.8% and UA of 64.4%.
32522547	3	20	theme	liquid	280:285	arg1	LS					301:302	LS	301:302	LS	301:302	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	3	20	theme	liquid	280:285	arg1	ratio					294:298	liquid: solid ratio	280:298	liquid: solid ratio (LS)	280:303	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	2	21	theme	Chardonnay	118:127	arg1	source					161:166	a source	159:166	a source of pectin	159:176	Chardonnay grape pomace was evaluated as a source of pectin.
32522547	2	21	theme	Chardonnay	118:127	arg1	pomace					135:140	Chardonnay grape pomace	118:140	Chardonnay grape pomace	118:140	Chardonnay grape pomace was evaluated as a source of pectin.
32522547	5	22	theme	optimized	644:652	arg1	GPOP					666:669	GPOP	666:669	GPOP	666:669	The experimental yield of the pectic fraction obtained under the optimized conditions (GPOP) was 11.1% and the UA was 56.8%.
32522547	5	22	theme	optimized	644:652	arg1	conditions					654:663	the optimized conditions	640:663	the optimized conditions (GPOP)	640:670	The experimental yield of the pectic fraction obtained under the optimized conditions (GPOP) was 11.1% and the UA was 56.8%.
32522547	8	23	theme	pectic	829:834	arg1	fraction					836:843	The starch-free pectic fraction	813:843	The starch-free pectic fraction	813:843	The starch-free pectic fraction was composed of 63.5% UA, 7.8% rhamnose, 6.0% arabinose, 13.6% galactose and minor amounts of other neutral monosaccharides.
32522547	9	24	contain	contained	973:981	arg2	pectin					998:1003	a low-methoxyl pectin	983:1003	a low-methoxyl pectin (degree of methyl-esterification 18.1%)	983:1043	It contained a low-methoxyl pectin (degree of methyl-esterification 18.1%) and had an average molar mass of 154,100 g/mol.
32522547	9	24	contain	contained	973:981	arg1	It					970:971	It	970:971	It	970:971	It contained a low-methoxyl pectin (degree of methyl-esterification 18.1%) and had an average molar mass of 154,100 g/mol.
32522547	3	25	theme	UA	335:336	arg1	content					339:345	uronic acid (UA) content	322:345	uronic acid (UA) content	322:345	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	3	26	dep	yield	312:316	arg1	the					308:310	the	308:310	the	308:310	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	5	27	theme	fraction	616:623	arg1	yield					596:600	The experimental yield	579:600	The experimental yield of the pectic fraction obtained under the optimized conditions (GPOP)	579:670	The experimental yield of the pectic fraction obtained under the optimized conditions (GPOP) was 11.1% and the UA was 56.8%.
32522547	5	27	theme	fraction	616:623	arg1	%					680:680	11.1%	676:680	11.1%	676:680	The experimental yield of the pectic fraction obtained under the optimized conditions (GPOP) was 11.1% and the UA was 56.8%.
32522547	9	28	theme	154,100 g/mol	1078:1090	arg1	mass					1070:1073	an average molar mass	1053:1073	an average molar mass of 154,100 g/mol	1053:1090	It contained a low-methoxyl pectin (degree of methyl-esterification 18.1%) and had an average molar mass of 154,100 g/mol.
32522547	3	29	dep	liquid	280:285	arg1	solid					288:292	solid	288:292	solid	288:292	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	3	30	theme	uronic	322:327	arg1	content					339:345	uronic acid (UA) content	322:345	uronic acid (UA) content	322:345	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	8	31	theme	arabinose	891:899	arg1	%					889:889	6.0% arabinose	886:899	6.0% arabinose	886:899	The starch-free pectic fraction was composed of 63.5% UA, 7.8% rhamnose, 6.0% arabinose, 13.6% galactose and minor amounts of other neutral monosaccharides.
32522547	8	31	theme	arabinose	891:899	arg1	monosaccharides					953:967	other neutral monosaccharides	939:967	other neutral monosaccharides	939:967	The starch-free pectic fraction was composed of 63.5% UA, 7.8% rhamnose, 6.0% arabinose, 13.6% galactose and minor amounts of other neutral monosaccharides.
32522547	1	32	theme	Chardonnay	82:91	arg1	Waste					111:115	a Winery Waste	102:115	a Winery Waste	102:115	Chardonnay) pomace, a Winery Waste.
32522547	1	32	theme	Chardonnay	82:91	arg1	pomace					94:99	Chardonnay) pomace	82:99	Chardonnay) pomace	82:99	Chardonnay) pomace, a Winery Waste.
32522547	3	33	theme	acid	329:332	arg1	content					339:345	uronic acid (UA) content	322:345	uronic acid (UA) content	322:345	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	3	34	theme	ratio	294:298	arg1	effect					241:246	the effect	237:246	the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution	237:398	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	8	35	theme	neutral	945:951	arg1	monosaccharides					953:967	other neutral monosaccharides	939:967	other neutral monosaccharides	939:967	The starch-free pectic fraction was composed of 63.5% UA, 7.8% rhamnose, 6.0% arabinose, 13.6% galactose and minor amounts of other neutral monosaccharides.
32522547	3	36	dep	time	266:269	arg1	Et					272:273	Et	272:273	Et	272:273	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	11	37	theme	galactose	1291:1299	arg1	chains					1248:1253	short chains	1242:1253	short chains	1242:1253	NMR analyses suggest that RG-I portion of α-GPOP is highly branched by short chains or single residues of arabinose and galactose.
32522547	11	37	theme	galactose	1291:1299	arg1	residues					1265:1272	single residues	1258:1272	single residues	1258:1272	NMR analyses suggest that RG-I portion of α-GPOP is highly branched by short chains or single residues of arabinose and galactose.
32522547	11	37	theme	galactose	1291:1299	arg1	galactose					1291:1299	galactose	1291:1299	galactose	1291:1299	NMR analyses suggest that RG-I portion of α-GPOP is highly branched by short chains or single residues of arabinose and galactose.
32522547	11	37	theme	galactose	1291:1299	arg1	arabinose					1277:1285	arabinose	1277:1285	arabinose	1277:1285	NMR analyses suggest that RG-I portion of α-GPOP is highly branched by short chains or single residues of arabinose and galactose.
32522547	6	38	contain	had	709:711	arg2	glucose					718:724	~25% glucose	713:724	~25% glucose	713:724	GPOP had ~25% glucose.
32522547	6	38	contain	had	709:711	arg1	GPOP					704:707	GPOP	704:707	GPOP	704:707	GPOP had ~25% glucose.
32522547	0	39	theme	acid-extraction	16:30	arg1	Optimization					0:11	Optimization	0:11	Optimization of acid-extraction of pectic fraction from grape (Vitis vinifera cv.	0:80	Optimization of acid-extraction of pectic fraction from grape (Vitis vinifera cv.
32522547	11	40	theme	arabinose	1277:1285	arg1	chains					1248:1253	short chains	1242:1253	short chains	1242:1253	NMR analyses suggest that RG-I portion of α-GPOP is highly branched by short chains or single residues of arabinose and galactose.
32522547	11	40	theme	arabinose	1277:1285	arg1	residues					1265:1272	single residues	1258:1272	single residues	1258:1272	NMR analyses suggest that RG-I portion of α-GPOP is highly branched by short chains or single residues of arabinose and galactose.
32522547	11	40	theme	arabinose	1277:1285	arg1	galactose					1291:1299	galactose	1291:1299	galactose	1291:1299	NMR analyses suggest that RG-I portion of α-GPOP is highly branched by short chains or single residues of arabinose and galactose.
32522547	11	40	theme	arabinose	1277:1285	arg1	arabinose					1277:1285	arabinose	1277:1285	arabinose	1277:1285	NMR analyses suggest that RG-I portion of α-GPOP is highly branched by short chains or single residues of arabinose and galactose.
32522547	10	41	theme	55.7	1109:1112	arg1	%					1113:1113	%	1113:1113	%	1113:1113	It consisted of 55.7% homogalacturonan and 35.2% rhamnogalacturonan I (RG-I).
32522547	11	42	theme	α-GPOP	1213:1218	arg1	portion					1202:1208	RG-I portion	1197:1208	RG-I portion of α-GPOP	1197:1218	NMR analyses suggest that RG-I portion of α-GPOP is highly branched by short chains or single residues of arabinose and galactose.
32522547	0	43	theme	fraction	42:49	arg1	acid-extraction					16:30	acid-extraction	16:30	acid-extraction of pectic fraction from grape (Vitis vinifera cv	16:79	Optimization of acid-extraction of pectic fraction from grape (Vitis vinifera cv.
32522547	4	44	theme	maximum	449:455	arg1	yield					457:461	the maximum yield	445:461	the maximum yield	445:461	The optimized extraction condition to reach the maximum yield and UA was pH = 2.08, Et = 135.23 min and LS = 35.11 ml/g, resulting in theoretical yield of 12.8% and UA of 64.4%.
32522547	9	45	theme	methyl-esterification	1016:1036	arg1	degree					1006:1011	degree	1006:1011	degree of methyl-esterification 18.1%	1006:1042	It contained a low-methoxyl pectin (degree of methyl-esterification 18.1%) and had an average molar mass of 154,100 g/mol.
32522547	0	46	dep	grape	56:60	arg1	vinifera					69:76	Vitis vinifera cv	63:79	Vitis vinifera cv	63:79	Optimization of acid-extraction of pectic fraction from grape (Vitis vinifera cv.
32522547	4	47	theme	theoretical	535:545	arg1	yield					547:551	theoretical yield	535:551	theoretical yield of 12.8% and UA of 64.4%	535:576	The optimized extraction condition to reach the maximum yield and UA was pH = 2.08, Et = 135.23 min and LS = 35.11 ml/g, resulting in theoretical yield of 12.8% and UA of 64.4%.
32522547	0	48	theme	pectic	35:40	arg1	fraction					42:49	pectic fraction	35:49	pectic fraction from grape (Vitis vinifera cv	35:79	Optimization of acid-extraction of pectic fraction from grape (Vitis vinifera cv.
32522547	4	49	theme	extraction	415:424	arg1	condition					426:434	The optimized extraction condition	401:434	The optimized extraction condition	401:434	The optimized extraction condition to reach the maximum yield and UA was pH = 2.08, Et = 135.23 min and LS = 35.11 ml/g, resulting in theoretical yield of 12.8% and UA of 64.4%.
32522547	5	50	theme	experimental	583:594	arg1	yield					596:600	The experimental yield	579:600	The experimental yield of the pectic fraction obtained under the optimized conditions (GPOP)	579:670	The experimental yield of the pectic fraction obtained under the optimized conditions (GPOP) was 11.1% and the UA was 56.8%.
32522547	5	50	theme	experimental	583:594	arg1	%					680:680	11.1%	676:680	11.1%	676:680	The experimental yield of the pectic fraction obtained under the optimized conditions (GPOP) was 11.1% and the UA was 56.8%.
32522547	11	51	theme	NMR	1171:1173	arg1	analyses					1175:1182	NMR analyses	1171:1182	NMR analyses	1171:1182	NMR analyses suggest that RG-I portion of α-GPOP is highly branched by short chains or single residues of arabinose and galactose.
32522547	8	52	theme	UA	867:868	arg1	%					865:865	63.5% UA	861:868	63.5% UA	861:868	The starch-free pectic fraction was composed of 63.5% UA, 7.8% rhamnose, 6.0% arabinose, 13.6% galactose and minor amounts of other neutral monosaccharides.
32522547	8	52	theme	UA	867:868	arg1	monosaccharides					953:967	other neutral monosaccharides	939:967	other neutral monosaccharides	939:967	The starch-free pectic fraction was composed of 63.5% UA, 7.8% rhamnose, 6.0% arabinose, 13.6% galactose and minor amounts of other neutral monosaccharides.
32522547	5	53	theme	pectic	609:614	arg1	fraction					616:623	the pectic fraction	605:623	the pectic fraction obtained under the optimized conditions (GPOP)	605:670	The experimental yield of the pectic fraction obtained under the optimized conditions (GPOP) was 11.1% and the UA was 56.8%.
32522547	4	54	theme	optimized	405:413	arg1	condition					426:434	The optimized extraction condition	401:434	The optimized extraction condition	401:434	The optimized extraction condition to reach the maximum yield and UA was pH = 2.08, Et = 135.23 min and LS = 35.11 ml/g, resulting in theoretical yield of 12.8% and UA of 64.4%.
32522547	6	55	theme	~25	713:715	arg1	%					716:716	%	716:716	%	716:716	GPOP had ~25% glucose.
32522547	3	56	theme	extraction	255:264	arg1	time					266:269	extraction time	255:269	extraction time (Et)	255:274	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	6	57	theme	%	716:716	arg1	glucose					718:724	~25% glucose	713:724	~25% glucose	713:724	GPOP had ~25% glucose.
32522547	9	58	theme	molar	1064:1068	arg1	mass					1070:1073	an average molar mass	1053:1073	an average molar mass of 154,100 g/mol	1053:1090	It contained a low-methoxyl pectin (degree of methyl-esterification 18.1%) and had an average molar mass of 154,100 g/mol.
32522547	3	59	theme	boiling	378:384	arg1	solution					391:398	boiling HNO3 solution	378:398	boiling HNO3 solution	378:398	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	2	60	theme	pectin	171:176	arg1	source					161:166	a source	159:166	a source of pectin	159:176	Chardonnay grape pomace was evaluated as a source of pectin.
32522547	2	60	theme	pectin	171:176	arg1	pomace					135:140	Chardonnay grape pomace	118:140	Chardonnay grape pomace	118:140	Chardonnay grape pomace was evaluated as a source of pectin.
32522547	1	61	theme	Winery	104:109	arg1	Waste					111:115	a Winery Waste	102:115	a Winery Waste	102:115	Chardonnay) pomace, a Winery Waste.
32522547	1	61	theme	Winery	104:109	arg1	pomace					94:99	Chardonnay) pomace	82:99	Chardonnay) pomace	82:99	Chardonnay) pomace, a Winery Waste.
32522547	10	62	theme	35.2	1136:1139	arg1	%					1140:1140	%	1140:1140	%	1140:1140	It consisted of 55.7% homogalacturonan and 35.2% rhamnogalacturonan I (RG-I).
32522547	8	63	theme	other	939:943	arg1	monosaccharides					953:967	other neutral monosaccharides	939:967	other neutral monosaccharides	939:967	The starch-free pectic fraction was composed of 63.5% UA, 7.8% rhamnose, 6.0% arabinose, 13.6% galactose and minor amounts of other neutral monosaccharides.
32522547	3	64	theme	pH	251:252	arg1	effect					241:246	the effect	237:246	the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution	237:398	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	11	65	theme	RG-I	1197:1200	arg1	portion					1202:1208	RG-I portion	1197:1208	RG-I portion of α-GPOP	1197:1218	NMR analyses suggest that RG-I portion of α-GPOP is highly branched by short chains or single residues of arabinose and galactose.
32522547	10	66	theme	rhamnogalacturonan	1142:1159	arg1	RG-I					1164:1167	RG-I	1164:1167	RG-I	1164:1167	It consisted of 55.7% homogalacturonan and 35.2% rhamnogalacturonan I (RG-I).
32522547	10	66	theme	rhamnogalacturonan	1142:1159	arg1	I					1161:1161	35.2% rhamnogalacturonan I	1136:1161	35.2% rhamnogalacturonan I (RG-I)	1136:1168	It consisted of 55.7% homogalacturonan and 35.2% rhamnogalacturonan I (RG-I).
32522547	3	67	theme	pectins	354:360	arg1	content					339:345	uronic acid (UA) content	322:345	uronic acid (UA) content	322:345	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	3	67	theme	pectins	354:360	arg1	yield					312:316	yield	312:316	yield	312:316	A central composite design was used in order to determine the effect of pH, extraction time (Et) and liquid: solid ratio (LS) on the yield and uronic acid (UA) content of the pectins extracted using boiling HNO3 solution.
32522547	0	68	theme	Vitis	63:67	arg1	vinifera					69:76	Vitis vinifera cv	63:79	Vitis vinifera cv	63:79	Optimization of acid-extraction of pectic fraction from grape (Vitis vinifera cv.
32522547	10	69	theme	%	1140:1140	arg1	RG-I					1164:1167	RG-I	1164:1167	RG-I	1164:1167	It consisted of 55.7% homogalacturonan and 35.2% rhamnogalacturonan I (RG-I).
32522547	10	69	theme	%	1140:1140	arg1	I					1161:1161	35.2% rhamnogalacturonan I	1136:1161	35.2% rhamnogalacturonan I (RG-I)	1136:1168	It consisted of 55.7% homogalacturonan and 35.2% rhamnogalacturonan I (RG-I).
32522547	4	70	theme	UA	566:567	arg1	yield					547:551	theoretical yield	535:551	theoretical yield of 12.8% and UA of 64.4%	535:576	The optimized extraction condition to reach the maximum yield and UA was pH = 2.08, Et = 135.23 min and LS = 35.11 ml/g, resulting in theoretical yield of 12.8% and UA of 64.4%.
32522547	11	71	theme	short	1242:1246	arg1	chains					1248:1253	short chains	1242:1253	short chains	1242:1253	NMR analyses suggest that RG-I portion of α-GPOP is highly branched by short chains or single residues of arabinose and galactose.
32522547	8	72	theme	galactose	908:916	arg1	monosaccharides					953:967	other neutral monosaccharides	939:967	other neutral monosaccharides	939:967	The starch-free pectic fraction was composed of 63.5% UA, 7.8% rhamnose, 6.0% arabinose, 13.6% galactose and minor amounts of other neutral monosaccharides.
32522547	8	72	theme	galactose	908:916	arg1	%					906:906	13.6% galactose	902:916	13.6% galactose	902:916	The starch-free pectic fraction was composed of 63.5% UA, 7.8% rhamnose, 6.0% arabinose, 13.6% galactose and minor amounts of other neutral monosaccharides.
32522547	9	73	theme	average	1056:1062	arg1	mass					1070:1073	an average molar mass	1053:1073	an average molar mass of 154,100 g/mol	1053:1090	It contained a low-methoxyl pectin (degree of methyl-esterification 18.1%) and had an average molar mass of 154,100 g/mol.
32522547	7	74	theme	fraction	796:803	arg1	α-GPOP					805:810	the fraction α-GPOP	792:810	the fraction α-GPOP	792:810	It was treated with α-amylase and amyloglucosidase, resulting in the fraction α-GPOP.
32522547	4	75	theme	%	576:576	arg1	UA					566:567	UA	566:567	UA	566:567	The optimized extraction condition to reach the maximum yield and UA was pH = 2.08, Et = 135.23 min and LS = 35.11 ml/g, resulting in theoretical yield of 12.8% and UA of 64.4%.
32522547	4	75	theme	%	576:576	arg1	%					576:576	64.4%	572:576	64.4%	572:576	The optimized extraction condition to reach the maximum yield and UA was pH = 2.08, Et = 135.23 min and LS = 35.11 ml/g, resulting in theoretical yield of 12.8% and UA of 64.4%.
32522547	4	75	theme	%	576:576	arg1	%					560:560	12.8%	556:560	12.8%	556:560	The optimized extraction condition to reach the maximum yield and UA was pH = 2.08, Et = 135.23 min and LS = 35.11 ml/g, resulting in theoretical yield of 12.8% and UA of 64.4%.
34473483	0	0	theme	Magnetic	110:117	arg1	Conversion					145:154	Magnetic UV Response Light-to-Heat Conversion	110:154	Magnetic UV Response Light-to-Heat Conversion	110:154	Design and Fabrication of Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials for Magnetic UV Response Light-to-Heat Conversion and Storage.
34473483	2	1	theme	energy	521:526	arg1	storage					528:534	phase-change energy storage	508:534	phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage	508:627	This research reports a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage.
34473483	10	2	used	used	1666:1669	arg2	sensors					1646:1652	ultraviolet-magnetic sensors	1625:1652	ultraviolet-magnetic sensors	1625:1652	This provided a way to prepare light-to-heat conversion-storage-release materials and ultraviolet-magnetic sensors that can be used in renewable resources.
34473483	10	2	used	used	1666:1669	arg2	materials					1611:1619	light-to-heat conversion-storage-release materials	1570:1619	light-to-heat conversion-storage-release materials	1570:1619	This provided a way to prepare light-to-heat conversion-storage-release materials and ultraviolet-magnetic sensors that can be used in renewable resources.
34473483	8	3	dep	values	1232:1237	arg1	enthalpies					1264:1273	melting/crystallization enthalpies	1240:1273	melting/crystallization enthalpies of 314.8 and 197.9 J/g	1240:1296	The differential scanning calorimetry curve and latent heat values (melting/crystallization enthalpies of 314.8 and 197.9 J/g, respectively) of MC-EP-ER-75 did not change after 100 cycles.
34473483	1	4	theme	global	268:273	arg1	shortage					282:289	the global energy shortage	264:289	the global energy shortage	264:289	The collection, storage, and use of energy and information are important issues for overcoming the global energy shortage while satisfying the demand for information transmission.
34473483	2	5	theme	phase-change	508:519	arg1	storage					528:534	phase-change energy storage	508:534	phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage	508:627	This research reports a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage.
34473483	6	6	theme	ER	946:947	arg1	loading					935:941	the loading	931:941	the loading of ER at a photothermal conversion efficiency of 79.67% and a light-to-heat conversion efficiency of 79.67%	931:1049	The MC-EP-ER-75 was able to store solar radiation-induced energy due to the loading of ER at a photothermal conversion efficiency of 79.67% and a light-to-heat conversion efficiency of 79.67%.
34473483	8	7	dep	calorimetry	1198:1208	arg1	curve					1210:1214	curve	1210:1214	curve	1210:1214	The differential scanning calorimetry curve and latent heat values (melting/crystallization enthalpies of 314.8 and 197.9 J/g, respectively) of MC-EP-ER-75 did not change after 100 cycles.
34473483	5	8	theme	thermal	831:837	arg1	conductivity					839:850	the low thermal conductivity	823:850	the low thermal conductivity of ER	823:856	The addition of nano-Fe3O4 compensated for the low thermal conductivity of ER.
34473483	4	9	with	combination	756:766	arg1	MC-EP					773:777	MC-EP	773:777	MC-EP	773:777	ER was used to form an effective combination with MC-EP.
34473483	1	10	theme	energy	275:280	arg1	shortage					282:289	the global energy shortage	264:289	the global energy shortage	264:289	The collection, storage, and use of energy and information are important issues for overcoming the global energy shortage while satisfying the demand for information transmission.
34473483	0	11	theme	Response	122:129	arg1	Conversion					145:154	Magnetic UV Response Light-to-Heat Conversion	110:154	Magnetic UV Response Light-to-Heat Conversion	110:154	Design and Fabrication of Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials for Magnetic UV Response Light-to-Heat Conversion and Storage.
34473483	6	12	theme	conversion	1019:1028	arg1	efficiency					1030:1039	a light-to-heat conversion efficiency	1003:1039	a light-to-heat conversion efficiency of 79.67%	1003:1049	The MC-EP-ER-75 was able to store solar radiation-induced energy due to the loading of ER at a photothermal conversion efficiency of 79.67% and a light-to-heat conversion efficiency of 79.67%.
34473483	0	13	theme	UV	119:120	arg1	Conversion					145:154	Magnetic UV Response Light-to-Heat Conversion	110:154	Magnetic UV Response Light-to-Heat Conversion	110:154	Design and Fabrication of Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials for Magnetic UV Response Light-to-Heat Conversion and Storage.
34473483	10	14	theme	renewable	1674:1682	arg1	resources					1684:1692	renewable resources	1674:1692	renewable resources	1674:1692	This provided a way to prepare light-to-heat conversion-storage-release materials and ultraviolet-magnetic sensors that can be used in renewable resources.
34473483	9	15	theme	excellent	1387:1395	arg1	magnetization					1408:1420	excellent saturation magnetization	1387:1420	excellent saturation magnetization	1387:1420	In addition, it exhibited excellent saturation magnetization, super-paramagnetism, and ultraviolet shielding, as well as a rapid response to the ultraviolet and magnetic fields.
34473483	6	16	theme	light-to-heat	1005:1017	arg1	efficiency					1030:1039	a light-to-heat conversion efficiency	1003:1039	a light-to-heat conversion efficiency of 79.67%	1003:1049	The MC-EP-ER-75 was able to store solar radiation-induced energy due to the loading of ER at a photothermal conversion efficiency of 79.67% and a light-to-heat conversion efficiency of 79.67%.
34473483	2	17	theme	magnetic	543:550	arg1	responses					568:576	the magnetic and ultraviolet responses	539:576	the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage	539:627	This research reports a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage.
34473483	2	18	theme	aerogel	450:456	arg1	composite					466:474	a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite	371:474	a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage	371:627	This research reports a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage.
34473483	8	19	theme	J/g	1294:1296	arg1	enthalpies					1264:1273	melting/crystallization enthalpies	1240:1273	melting/crystallization enthalpies of 314.8 and 197.9 J/g	1240:1296	The differential scanning calorimetry curve and latent heat values (melting/crystallization enthalpies of 314.8 and 197.9 J/g, respectively) of MC-EP-ER-75 did not change after 100 cycles.
34473483	0	20	theme	Light-to-Heat	131:143	arg1	Conversion					145:154	Magnetic UV Response Light-to-Heat Conversion	110:154	Magnetic UV Response Light-to-Heat Conversion	110:154	Design and Fabrication of Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials for Magnetic UV Response Light-to-Heat Conversion and Storage.
34473483	8	21	theme	melting/crystallization	1240:1262	arg1	enthalpies					1264:1273	melting/crystallization enthalpies	1240:1273	melting/crystallization enthalpies of 314.8 and 197.9 J/g	1240:1296	The differential scanning calorimetry curve and latent heat values (melting/crystallization enthalpies of 314.8 and 197.9 J/g, respectively) of MC-EP-ER-75 did not change after 100 cycles.
34473483	2	22	contain	has	481:483	arg1	composite					466:474	a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite	371:474	a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage	371:627	This research reports a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage.
34473483	2	22	contain	has	481:483	arg2	characteristics					489:503	the characteristics	485:503	the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage	485:627	This research reports a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage.
34473483	5	23	theme	ER	855:856	arg1	conductivity					839:850	the low thermal conductivity	823:850	the low thermal conductivity of ER	823:856	The addition of nano-Fe3O4 compensated for the low thermal conductivity of ER.
34473483	9	24	theme	saturation	1397:1406	arg1	magnetization					1408:1420	excellent saturation magnetization	1387:1420	excellent saturation magnetization	1387:1420	In addition, it exhibited excellent saturation magnetization, super-paramagnetism, and ultraviolet shielding, as well as a rapid response to the ultraviolet and magnetic fields.
34473483	8	25	theme	latent	1220:1225	arg1	heat					1227:1230	latent heat	1220:1230	latent heat	1220:1230	The differential scanning calorimetry curve and latent heat values (melting/crystallization enthalpies of 314.8 and 197.9 J/g, respectively) of MC-EP-ER-75 did not change after 100 cycles.
34473483	10	26	theme	ultraviolet-magnetic	1625:1644	arg1	sensors					1646:1652	ultraviolet-magnetic sensors	1625:1652	ultraviolet-magnetic sensors	1625:1652	This provided a way to prepare light-to-heat conversion-storage-release materials and ultraviolet-magnetic sensors that can be used in renewable resources.
34473483	10	27	theme	conversion-storage-release	1584:1609	arg1	materials					1611:1619	light-to-heat conversion-storage-release materials	1570:1619	light-to-heat conversion-storage-release materials	1570:1619	This provided a way to prepare light-to-heat conversion-storage-release materials and ultraviolet-magnetic sensors that can be used in renewable resources.
34473483	2	28	theme	-functionalized	403:417	arg1	composite					466:474	a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite	371:474	a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage	371:627	This research reports a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage.
34473483	3	29	from	structures	702:711	arg1	MC-EP					716:720	MC-EP	716:720	MC-EP	716:720	The nano-Fe3O4 particles in MC-EP-ER-75 were fixed and filled into pore structures in MC-EP.
34473483	6	30	from	efficiency	1030:1039	arg1	loading					935:941	the loading	931:941	the loading of ER at a photothermal conversion efficiency of 79.67% and a light-to-heat conversion efficiency of 79.67%	931:1049	The MC-EP-ER-75 was able to store solar radiation-induced energy due to the loading of ER at a photothermal conversion efficiency of 79.67% and a light-to-heat conversion efficiency of 79.67%.
34473483	1	31	theme	energy	205:210	arg1	issues					242:247	important issues	232:247	important issues for overcoming the global energy shortage while satisfying the demand for information transmission	232:346	The collection, storage, and use of energy and information are important issues for overcoming the global energy shortage while satisfying the demand for information transmission.
34473483	1	31	theme	energy	205:210	arg1	storage					185:191	storage	185:191	storage	185:191	The collection, storage, and use of energy and information are important issues for overcoming the global energy shortage while satisfying the demand for information transmission.
34473483	1	31	theme	energy	205:210	arg1	use					198:200	use	198:200	use	198:200	The collection, storage, and use of energy and information are important issues for overcoming the global energy shortage while satisfying the demand for information transmission.
34473483	1	31	theme	energy	205:210	arg1	collection					173:182	collection	173:182	collection	173:182	The collection, storage, and use of energy and information are important issues for overcoming the global energy shortage while satisfying the demand for information transmission.
34473483	8	32	theme	calorimetry	1198:1208	arg1	values					1232:1237	The differential scanning calorimetry curve and latent heat values	1172:1237	The differential scanning calorimetry curve and latent heat values (melting/crystallization enthalpies of 314.8 and 197.9 J/g, respectively) of MC-EP-ER-75	1172:1326	The differential scanning calorimetry curve and latent heat values (melting/crystallization enthalpies of 314.8 and 197.9 J/g, respectively) of MC-EP-ER-75 did not change after 100 cycles.
34473483	2	33	theme	light-to-heat	592:604	arg1	conversion					606:615	light-to-heat conversion	592:615	light-to-heat conversion	592:615	This research reports a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage.
34473483	4	34	used	used	730:733	arg2	ER					723:724	ER	723:724	ER	723:724	ER was used to form an effective combination with MC-EP.
34473483	1	35	dep	collection	173:182	arg1	The					169:171	The	169:171	The	169:171	The collection, storage, and use of energy and information are important issues for overcoming the global energy shortage while satisfying the demand for information transmission.
34473483	2	36	theme	MC-EP	459:463	arg1	composite					466:474	a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite	371:474	a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage	371:627	This research reports a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage.
34473483	6	37	theme	%	997:997	arg1	efficiency					978:987	a photothermal conversion efficiency	952:987	a photothermal conversion efficiency of 79.67%	952:997	The MC-EP-ER-75 was able to store solar radiation-induced energy due to the loading of ER at a photothermal conversion efficiency of 79.67% and a light-to-heat conversion efficiency of 79.67%.
34473483	6	37	theme	%	997:997	arg1	efficiency					1030:1039	a light-to-heat conversion efficiency	1003:1039	a light-to-heat conversion efficiency of 79.67%	1003:1049	The MC-EP-ER-75 was able to store solar radiation-induced energy due to the loading of ER at a photothermal conversion efficiency of 79.67% and a light-to-heat conversion efficiency of 79.67%.
34473483	7	38	theme	analysis	1091:1098	arg1	results					1056:1062	The results	1052:1062	The results of thermal stability (TGA) analysis	1052:1098	The results of thermal stability (TGA) analysis showed that MC-EP-ER-75 was thermally degraded acceptably below 200 °C.
34473483	3	39	theme	pore	697:700	arg1	structures					702:711	pore structures	697:711	pore structures in MC-EP	697:720	The nano-Fe3O4 particles in MC-EP-ER-75 were fixed and filled into pore structures in MC-EP.
34473483	10	40	theme	light-to-heat	1570:1582	arg1	materials					1611:1619	light-to-heat conversion-storage-release materials	1570:1619	light-to-heat conversion-storage-release materials	1570:1619	This provided a way to prepare light-to-heat conversion-storage-release materials and ultraviolet-magnetic sensors that can be used in renewable resources.
34473483	3	41	from	particles	645:653	arg1	MC-EP-ER-75					658:668	MC-EP-ER-75	658:668	MC-EP-ER-75	658:668	The nano-Fe3O4 particles in MC-EP-ER-75 were fixed and filled into pore structures in MC-EP.
34473483	1	42	theme	information	216:226	arg1	issues					242:247	important issues	232:247	important issues for overcoming the global energy shortage while satisfying the demand for information transmission	232:346	The collection, storage, and use of energy and information are important issues for overcoming the global energy shortage while satisfying the demand for information transmission.
34473483	1	42	theme	information	216:226	arg1	storage					185:191	storage	185:191	storage	185:191	The collection, storage, and use of energy and information are important issues for overcoming the global energy shortage while satisfying the demand for information transmission.
34473483	1	42	theme	information	216:226	arg1	use					198:200	use	198:200	use	198:200	The collection, storage, and use of energy and information are important issues for overcoming the global energy shortage while satisfying the demand for information transmission.
34473483	1	42	theme	information	216:226	arg1	collection					173:182	collection	173:182	collection	173:182	The collection, storage, and use of energy and information are important issues for overcoming the global energy shortage while satisfying the demand for information transmission.
34473483	0	43	theme	Methyl	55:60	arg1	Materials					96:104	Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials	26:104	Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials	26:104	Design and Fabrication of Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials for Magnetic UV Response Light-to-Heat Conversion and Storage.
34473483	9	44	theme	magnetic	1522:1529	arg1	fields					1531:1536	the ultraviolet and magnetic fields	1502:1536	fields	1531:1536	In addition, it exhibited excellent saturation magnetization, super-paramagnetism, and ultraviolet shielding, as well as a rapid response to the ultraviolet and magnetic fields.
34473483	2	45	theme	requisite	578:586	arg1	responses					568:576	the magnetic and ultraviolet responses	539:576	the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage	539:627	This research reports a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage.
34473483	6	46	theme	radiation-induced	899:915	arg1	energy					917:922	solar radiation-induced energy	893:922	solar radiation-induced energy	893:922	The MC-EP-ER-75 was able to store solar radiation-induced energy due to the loading of ER at a photothermal conversion efficiency of 79.67% and a light-to-heat conversion efficiency of 79.67%.
34473483	9	47	theme	rapid	1484:1488	arg1	response					1490:1497	a rapid response	1482:1497	a rapid response to the ultraviolet and magnetic fields	1482:1536	In addition, it exhibited excellent saturation magnetization, super-paramagnetism, and ultraviolet shielding, as well as a rapid response to the ultraviolet and magnetic fields.
34473483	2	48	theme	cellulose	440:448	arg1	composite					466:474	a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite	371:474	a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage	371:627	This research reports a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage.
34473483	0	49	theme	Epichlorohydrin-Cross-Linked	26:53	arg1	Materials					96:104	Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials	26:104	Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials	26:104	Design and Fabrication of Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials for Magnetic UV Response Light-to-Heat Conversion and Storage.
34473483	1	50	theme	information	323:333	arg1	transmission					335:346	information transmission	323:346	information transmission	323:346	The collection, storage, and use of energy and information are important issues for overcoming the global energy shortage while satisfying the demand for information transmission.
34473483	5	51	theme	low	827:829	arg1	conductivity					839:850	the low thermal conductivity	823:850	the low thermal conductivity of ER	823:856	The addition of nano-Fe3O4 compensated for the low thermal conductivity of ER.
34473483	6	52	theme	solar	893:897	arg1	energy					917:922	solar radiation-induced energy	893:922	solar radiation-induced energy	893:922	The MC-EP-ER-75 was able to store solar radiation-induced energy due to the loading of ER at a photothermal conversion efficiency of 79.67% and a light-to-heat conversion efficiency of 79.67%.
34473483	9	53	theme	ultraviolet	1448:1458	arg1	shielding					1460:1468	ultraviolet shielding	1448:1468	ultraviolet shielding	1448:1468	In addition, it exhibited excellent saturation magnetization, super-paramagnetism, and ultraviolet shielding, as well as a rapid response to the ultraviolet and magnetic fields.
34473483	1	54	theme	important	232:240	arg1	issues					242:247	important issues	232:247	important issues for overcoming the global energy shortage while satisfying the demand for information transmission	232:346	The collection, storage, and use of energy and information are important issues for overcoming the global energy shortage while satisfying the demand for information transmission.
34473483	1	54	theme	important	232:240	arg1	storage					185:191	storage	185:191	storage	185:191	The collection, storage, and use of energy and information are important issues for overcoming the global energy shortage while satisfying the demand for information transmission.
34473483	1	54	theme	important	232:240	arg1	use					198:200	use	198:200	use	198:200	The collection, storage, and use of energy and information are important issues for overcoming the global energy shortage while satisfying the demand for information transmission.
34473483	1	54	theme	important	232:240	arg1	collection					173:182	collection	173:182	collection	173:182	The collection, storage, and use of energy and information are important issues for overcoming the global energy shortage while satisfying the demand for information transmission.
34473483	0	55	theme	Aerogel-Based	72:84	arg1	Materials					96:104	Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials	26:104	Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials	26:104	Design and Fabrication of Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials for Magnetic UV Response Light-to-Heat Conversion and Storage.
34473483	2	56	theme	ultraviolet	556:566	arg1	responses					568:576	the magnetic and ultraviolet responses	539:576	the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage	539:627	This research reports a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage.
34473483	7	57	theme	thermal	1067:1073	arg1	TGA					1086:1088	TGA	1086:1088	TGA	1086:1088	The results of thermal stability (TGA) analysis showed that MC-EP-ER-75 was thermally degraded acceptably below 200 °C.
34473483	7	57	theme	thermal	1067:1073	arg1	stability					1075:1083	thermal stability	1067:1083	thermal stability (TGA) analysis	1067:1098	The results of thermal stability (TGA) analysis showed that MC-EP-ER-75 was thermally degraded acceptably below 200 °C.
34473483	2	58	theme	storage	528:534	arg1	characteristics					489:503	the characteristics	485:503	the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage	485:627	This research reports a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage.
34473483	4	59	theme	effective	746:754	arg1	combination					756:766	an effective combination	743:766	an effective combination with MC-EP	743:777	ER was used to form an effective combination with MC-EP.
34473483	0	60	theme	Cellulose	62:70	arg1	Materials					96:104	Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials	26:104	Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials	26:104	Design and Fabrication of Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials for Magnetic UV Response Light-to-Heat Conversion and Storage.
34473483	6	61	theme	conversion	967:976	arg1	efficiency					978:987	a photothermal conversion efficiency	952:987	a photothermal conversion efficiency of 79.67%	952:997	The MC-EP-ER-75 was able to store solar radiation-induced energy due to the loading of ER at a photothermal conversion efficiency of 79.67% and a light-to-heat conversion efficiency of 79.67%.
34473483	7	62	theme	stability	1075:1083	arg1	analysis					1091:1098	thermal stability (TGA) analysis	1067:1098	thermal stability (TGA) analysis	1067:1098	The results of thermal stability (TGA) analysis showed that MC-EP-ER-75 was thermally degraded acceptably below 200 °C.
34473483	3	63	theme	nano-Fe3O4	634:643	arg1	particles					645:653	The nano-Fe3O4 particles	630:653	The nano-Fe3O4 particles in MC-EP-ER-75	630:668	The nano-Fe3O4 particles in MC-EP-ER-75 were fixed and filled into pore structures in MC-EP.
34473483	8	64	theme	MC-EP-ER-75	1316:1326	arg1	values					1232:1237	The differential scanning calorimetry curve and latent heat values	1172:1237	The differential scanning calorimetry curve and latent heat values (melting/crystallization enthalpies of 314.8 and 197.9 J/g, respectively) of MC-EP-ER-75	1172:1326	The differential scanning calorimetry curve and latent heat values (melting/crystallization enthalpies of 314.8 and 197.9 J/g, respectively) of MC-EP-ER-75 did not change after 100 cycles.
34473483	6	65	theme	photothermal	954:965	arg1	efficiency					978:987	a photothermal conversion efficiency	952:987	a photothermal conversion efficiency of 79.67%	952:997	The MC-EP-ER-75 was able to store solar radiation-induced energy due to the loading of ER at a photothermal conversion efficiency of 79.67% and a light-to-heat conversion efficiency of 79.67%.
34473483	2	66	dep	-functionalized	403:417	arg1	erythritol					388:397	erythritol	388:397	erythritol	388:397	This research reports a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage.
34473483	2	66	dep	-functionalized	403:417	arg1	nano-Fe3O4					373:382	nano-Fe3O4	373:382	nano-Fe3O4	373:382	This research reports a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage.
34473483	2	66	dep	-functionalized	403:417	arg1	cross-linked					420:431	cross-linked	420:431	cross-linked	420:431	This research reports a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage.
34473483	5	67	theme	nano-Fe3O4	796:805	arg1	addition					784:791	The addition	780:791	The addition of nano-Fe3O4	780:805	The addition of nano-Fe3O4 compensated for the low thermal conductivity of ER.
34473483	8	68	theme	scanning	1189:1196	arg1	calorimetry					1198:1208	The differential scanning calorimetry curve and latent heat	1172:1230	calorimetry	1198:1208	The differential scanning calorimetry curve and latent heat values (melting/crystallization enthalpies of 314.8 and 197.9 J/g, respectively) of MC-EP-ER-75 did not change after 100 cycles.
34473483	6	69	from	efficiency	978:987	arg1	loading					935:941	the loading	931:941	the loading of ER at a photothermal conversion efficiency of 79.67% and a light-to-heat conversion efficiency of 79.67%	931:1049	The MC-EP-ER-75 was able to store solar radiation-induced energy due to the loading of ER at a photothermal conversion efficiency of 79.67% and a light-to-heat conversion efficiency of 79.67%.
34473483	0	70	theme	Materials	96:104	arg1	Fabrication					11:21	Fabrication	11:21	Fabrication	11:21	Design and Fabrication of Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials for Magnetic UV Response Light-to-Heat Conversion and Storage.
34473483	0	70	theme	Materials	96:104	arg1	Design					0:5	Design	0:5	Design	0:5	Design and Fabrication of Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials for Magnetic UV Response Light-to-Heat Conversion and Storage.
34473483	2	71	theme	methyl	433:438	arg1	composite					466:474	a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite	371:474	a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage	371:627	This research reports a nano-Fe3O4 and erythritol (ER)-functionalized, cross-linked methyl cellulose aerogel (MC-EP) composite that has the characteristics of phase-change energy storage as the magnetic and ultraviolet responses requisite for light-to-heat conversion and storage.
34473483	9	72	theme	ultraviolet	1506:1516	arg1	fields					1531:1536	the ultraviolet and magnetic fields	1502:1536	fields	1531:1536	In addition, it exhibited excellent saturation magnetization, super-paramagnetism, and ultraviolet shielding, as well as a rapid response to the ultraviolet and magnetic fields.
34473483	6	73	theme	%	1049:1049	arg1	efficiency					978:987	a photothermal conversion efficiency	952:987	a photothermal conversion efficiency of 79.67%	952:997	The MC-EP-ER-75 was able to store solar radiation-induced energy due to the loading of ER at a photothermal conversion efficiency of 79.67% and a light-to-heat conversion efficiency of 79.67%.
34473483	6	73	theme	%	1049:1049	arg1	efficiency					1030:1039	a light-to-heat conversion efficiency	1003:1039	a light-to-heat conversion efficiency of 79.67%	1003:1049	The MC-EP-ER-75 was able to store solar radiation-induced energy due to the loading of ER at a photothermal conversion efficiency of 79.67% and a light-to-heat conversion efficiency of 79.67%.
34473483	0	74	theme	Composite	86:94	arg1	Materials					96:104	Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials	26:104	Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials	26:104	Design and Fabrication of Epichlorohydrin-Cross-Linked Methyl Cellulose Aerogel-Based Composite Materials for Magnetic UV Response Light-to-Heat Conversion and Storage.
34473483	8	75	theme	heat	1227:1230	arg1	values					1232:1237	The differential scanning calorimetry curve and latent heat values	1172:1237	The differential scanning calorimetry curve and latent heat values (melting/crystallization enthalpies of 314.8 and 197.9 J/g, respectively) of MC-EP-ER-75	1172:1326	The differential scanning calorimetry curve and latent heat values (melting/crystallization enthalpies of 314.8 and 197.9 J/g, respectively) of MC-EP-ER-75 did not change after 100 cycles.
34473483	8	76	theme	differential	1176:1187	arg1	calorimetry					1198:1208	The differential scanning calorimetry curve and latent heat	1172:1230	calorimetry	1198:1208	The differential scanning calorimetry curve and latent heat values (melting/crystallization enthalpies of 314.8 and 197.9 J/g, respectively) of MC-EP-ER-75 did not change after 100 cycles.
33492206	16	0	theme	laboratory	2485:2494	arg1	media					2496:2500	laboratory media	2485:2500	laboratory media void of human matrix components	2485:2532	We suggest that the assumed importance of polysaccharides for biofilm formation is an artefact from studying biofilms in laboratory media void of human matrix components.
33492206	17	1	theme	epidermidis	2567:2577	arg1	aggregation					2549:2559	The cell-cell aggregation	2535:2559	The cell-cell aggregation of S. epidermidis	2535:2577	The cell-cell aggregation of S. epidermidis can be activated by host factors without relying on either of the major adhesins, PIA and Embp, indicating a need to revisit the basic question of how S. epidermidis deploys self-produced and host-derived matrix components to form antibiotic-tolerant biofilms in vivo.
33492206	5	2	theme	laboratory	673:682	arg1	media					684:688	laboratory media	673:688	laboratory media	673:688	We hypothesize that the dichotomic regulation of S. epidermidis adhesins is linked to whether it is inside a host or not, and that in vitro biofilm investigations in laboratory media may not reflect actual biofilms in vivo.Aim.
33492206	16	3	theme	void	2502:2505	arg1	media					2496:2500	laboratory media	2485:2500	laboratory media void of human matrix components	2485:2532	We suggest that the assumed importance of polysaccharides for biofilm formation is an artefact from studying biofilms in laboratory media void of human matrix components.
33492206	11	4	from	phenotype	1829:1837	arg1	presence					1846:1853	the presence	1842:1853	the presence of human plasma	1842:1869	However, these observations were not representative of the biofilm phenotype in the presence of human plasma.
33492206	5	5	theme	dichotomic	531:540	arg1	regulation					542:551	the dichotomic regulation	527:551	the dichotomic regulation of S. epidermidis adhesins	527:578	We hypothesize that the dichotomic regulation of S. epidermidis adhesins is linked to whether it is inside a host or not, and that in vitro biofilm investigations in laboratory media may not reflect actual biofilms in vivo.Aim.
33492206	15	6	theme	vancomycin	2311:2320	arg1	penetration					2322:2332	vancomycin penetration	2311:2332	vancomycin penetration	2311:2332	Despite stark differences in the amount and composition of biofilms formed by polysaccharide-producing and Embp-producing strains in different media, there were no differences in vancomycin penetration or susceptibility.Conclusion.
33492206	15	7	theme	Embp-producing	2239:2252	arg1	strains					2254:2260	polysaccharide-producing and Embp-producing strains	2210:2260	polysaccharide-producing and Embp-producing strains	2210:2260	Despite stark differences in the amount and composition of biofilms formed by polysaccharide-producing and Embp-producing strains in different media, there were no differences in vancomycin penetration or susceptibility.Conclusion.
33492206	2	8	from	predominant	184:194	arg1	infections					218:227	implant-associated infections	199:227	implant-associated infections due to its capability to form biofilms	199:266	Staphylococcus epidermidis is predominant in implant-associated infections due to its capability to form biofilms.
33492206	16	9	theme	polysaccharides	2406:2420	arg1	importance					2392:2401	the assumed importance	2380:2401	the assumed importance of polysaccharides for biofilm formation	2380:2442	We suggest that the assumed importance of polysaccharides for biofilm formation is an artefact from studying biofilms in laboratory media void of human matrix components.
33492206	16	9	theme	polysaccharides	2406:2420	arg1	artefact					2450:2457	an artefact	2447:2457	an artefact from studying biofilms in laboratory media void of human matrix components	2447:2532	We suggest that the assumed importance of polysaccharides for biofilm formation is an artefact from studying biofilms in laboratory media void of human matrix components.
33492206	6	10	theme	biofilm	873:879	arg1	formation					881:889	biofilm formation	873:889	biofilm formation	873:889	We address the importance of PIA and Embp in biofilm grown in 'humanized' media to understand if these components play different roles in biofilm formation under conditions where bacteria can incorporate host proteins in the biofilm matrix.Methodology.
33492206	17	11	theme	self-produced	2753:2765	arg1	components					2791:2800	self-produced and host-derived matrix components	2753:2800	self-produced and host-derived matrix components	2753:2800	The cell-cell aggregation of S. epidermidis can be activated by host factors without relying on either of the major adhesins, PIA and Embp, indicating a need to revisit the basic question of how S. epidermidis deploys self-produced and host-derived matrix components to form antibiotic-tolerant biofilms in vivo.
33492206	16	12	theme	biofilm	2426:2432	arg1	formation					2434:2442	biofilm formation	2426:2442	biofilm formation	2426:2442	We suggest that the assumed importance of polysaccharides for biofilm formation is an artefact from studying biofilms in laboratory media void of human matrix components.
33492206	17	13	theme	major	2645:2649	arg1	PIA					2661:2663	PIA	2661:2663	PIA	2661:2663	The cell-cell aggregation of S. epidermidis can be activated by host factors without relying on either of the major adhesins, PIA and Embp, indicating a need to revisit the basic question of how S. epidermidis deploys self-produced and host-derived matrix components to form antibiotic-tolerant biofilms in vivo.
33492206	17	13	theme	major	2645:2649	arg1	adhesins					2651:2658	the major adhesins	2641:2658	the major adhesins	2641:2658	The cell-cell aggregation of S. epidermidis can be activated by host factors without relying on either of the major adhesins, PIA and Embp, indicating a need to revisit the basic question of how S. epidermidis deploys self-produced and host-derived matrix components to form antibiotic-tolerant biofilms in vivo.
33492206	17	13	theme	major	2645:2649	arg1	Embp					2669:2672	Embp	2669:2672	Embp	2669:2672	The cell-cell aggregation of S. epidermidis can be activated by host factors without relying on either of the major adhesins, PIA and Embp, indicating a need to revisit the basic question of how S. epidermidis deploys self-produced and host-derived matrix components to form antibiotic-tolerant biofilms in vivo.
33492206	7	14	theme	human	1220:1224	arg1	plasma					1226:1231	plasma	1226:1231	plasma	1226:1231	S. epidermidis 1585 WT (deficient in icaADBC), and derivative strains that either lack embp, express embp from an inducible promotor, or express icaADBC from a plasmid, were cultivated in standard laboratory media, or in media with human plasma or serum.
33492206	6	15	theme	biofilm	960:966	arg1	matrix.Methodology					968:985	the biofilm matrix.Methodology	956:985	the biofilm matrix.Methodology	956:985	We address the importance of PIA and Embp in biofilm grown in 'humanized' media to understand if these components play different roles in biofilm formation under conditions where bacteria can incorporate host proteins in the biofilm matrix.Methodology.
33492206	3	16	theme	biofilm	306:312	arg1	development					314:324	biofilm development	306:324	biofilm development	306:324	It can deploy several strategies for biofilm development using either polysaccharide intercellular adhesin (PIA), extracellular DNA (eDNA) and/or proteins, such as the extracellular matrix-binding protein (Embp).
33492206	6	17	dep	media	809:813	arg1	understand					818:827	understand	818:827	to understand if these components play different roles in biofilm formation under conditions where bacteria can incorporate host proteins in the biofilm matrix.Methodology	815:985	We address the importance of PIA and Embp in biofilm grown in 'humanized' media to understand if these components play different roles in biofilm formation under conditions where bacteria can incorporate host proteins in the biofilm matrix.Methodology.
33492206	11	18	theme	phenotype	1829:1837	arg1	representative					1799:1812	representative	1799:1812	representative	1799:1812	However, these observations were not representative of the biofilm phenotype in the presence of human plasma.
33492206	15	19	theme	stark	2140:2144	arg1	differences					2146:2156	stark differences	2140:2156	stark differences in the amount and composition of biofilms formed by polysaccharide-producing and Embp-producing strains in different media	2140:2279	Despite stark differences in the amount and composition of biofilms formed by polysaccharide-producing and Embp-producing strains in different media, there were no differences in vancomycin penetration or susceptibility.Conclusion.
33492206	5	20	dep	S.	556:557	arg1	epidermidis					559:569	epidermidis	559:569	epidermidis	559:569	We hypothesize that the dichotomic regulation of S. epidermidis adhesins is linked to whether it is inside a host or not, and that in vitro biofilm investigations in laboratory media may not reflect actual biofilms in vivo.Aim.
33492206	6	21	theme	host	939:942	arg1	proteins					944:951	host proteins	939:951	host proteins	939:951	We address the importance of PIA and Embp in biofilm grown in 'humanized' media to understand if these components play different roles in biofilm formation under conditions where bacteria can incorporate host proteins in the biofilm matrix.Methodology.
33492206	3	22	theme	polysaccharide	339:352	arg1	PIA					377:379	PIA	377:379	PIA	377:379	It can deploy several strategies for biofilm development using either polysaccharide intercellular adhesin (PIA), extracellular DNA (eDNA) and/or proteins, such as the extracellular matrix-binding protein (Embp).
33492206	3	22	theme	polysaccharide	339:352	arg1	adhesin					368:374	either polysaccharide intercellular adhesin	332:374	either polysaccharide intercellular adhesin (PIA)	332:380	It can deploy several strategies for biofilm development using either polysaccharide intercellular adhesin (PIA), extracellular DNA (eDNA) and/or proteins, such as the extracellular matrix-binding protein (Embp).
33492206	9	23	dep	factors	1528:1534	arg1	response					1511:1518	response	1511:1518	response	1511:1518	Finally, we quantified the initiation of biofilms as suspended aggregates in response to host factors to determine how quickly the cells aggregate in response to the host environment and reach a size that protects them from phagocytosis.Results.
33492206	7	24	theme	derivative	1039:1048	arg1	strains					1050:1056	derivative strains	1039:1056	derivative strains that either lack embp, express embp from an inducible promotor, or express icaADBC from a plasmid	1039:1154	S. epidermidis 1585 WT (deficient in icaADBC), and derivative strains that either lack embp, express embp from an inducible promotor, or express icaADBC from a plasmid, were cultivated in standard laboratory media, or in media with human plasma or serum.
33492206	9	25	theme	suspended	1487:1495	arg1	aggregates					1497:1506	suspended aggregates	1487:1506	suspended aggregates	1487:1506	Finally, we quantified the initiation of biofilms as suspended aggregates in response to host factors to determine how quickly the cells aggregate in response to the host environment and reach a size that protects them from phagocytosis.Results.
33492206	9	25	theme	suspended	1487:1495	arg1	initiation					1461:1470	the initiation	1457:1470	the initiation of biofilms	1457:1482	Finally, we quantified the initiation of biofilms as suspended aggregates in response to host factors to determine how quickly the cells aggregate in response to the host environment and reach a size that protects them from phagocytosis.Results.
33492206	17	26	link	host-derived	2771:2782	arg1	components					2791:2800	self-produced and host-derived matrix components	2753:2800	self-produced and host-derived matrix components	2753:2800	The cell-cell aggregation of S. epidermidis can be activated by host factors without relying on either of the major adhesins, PIA and Embp, indicating a need to revisit the basic question of how S. epidermidis deploys self-produced and host-derived matrix components to form antibiotic-tolerant biofilms in vivo.
33492206	8	27	theme	antimicrobial	1281:1293	arg1	penetration					1295:1305	antimicrobial penetration	1281:1305	antimicrobial penetration	1281:1305	The amount, structure, elasticity and antimicrobial penetration of biofilms was quantified to describe structural differences caused by the different matrix components and growth conditions.
33492206	7	28	dep	S.	988:989	arg1	epidermidis					991:1001	epidermidis	991:1001	epidermidis	991:1001	S. epidermidis 1585 WT (deficient in icaADBC), and derivative strains that either lack embp, express embp from an inducible promotor, or express icaADBC from a plasmid, were cultivated in standard laboratory media, or in media with human plasma or serum.
33492206	0	29	theme	extracellular	54:66	arg1	protein					83:89	extracellular matrix-binding protein	54:89	extracellular matrix-binding protein	54:89	Host factors abolish the need for polysaccharides and extracellular matrix-binding protein in Staphylococcus epidermidis biofilm formation.
33492206	17	30	theme	basic	2708:2712	arg1	question					2714:2721	the basic question	2704:2721	the basic question of how S. epidermidis deploys self-produced and host-derived matrix components to form antibiotic-tolerant biofilms in vivo	2704:2845	The cell-cell aggregation of S. epidermidis can be activated by host factors without relying on either of the major adhesins, PIA and Embp, indicating a need to revisit the basic question of how S. epidermidis deploys self-produced and host-derived matrix components to form antibiotic-tolerant biofilms in vivo.
33492206	9	31	theme	host	1523:1526	arg1	factors					1528:1534	host factors	1523:1534	host factors	1523:1534	Finally, we quantified the initiation of biofilms as suspended aggregates in response to host factors to determine how quickly the cells aggregate in response to the host environment and reach a size that protects them from phagocytosis.Results.
33492206	0	32	theme	Staphylococcus	94:107	arg1	formation					129:137	Staphylococcus epidermidis biofilm formation	94:137	Staphylococcus epidermidis biofilm formation	94:137	Host factors abolish the need for polysaccharides and extracellular matrix-binding protein in Staphylococcus epidermidis biofilm formation.
33492206	0	33	theme	biofilm	121:127	arg1	formation					129:137	Staphylococcus epidermidis biofilm formation	94:137	Staphylococcus epidermidis biofilm formation	94:137	Host factors abolish the need for polysaccharides and extracellular matrix-binding protein in Staphylococcus epidermidis biofilm formation.
33492206	0	34	dep	Staphylococcus	94:107	arg1	epidermidis					109:119	epidermidis	109:119	epidermidis	109:119	Host factors abolish the need for polysaccharides and extracellular matrix-binding protein in Staphylococcus epidermidis biofilm formation.
33492206	17	35	theme	host-derived	2771:2782	arg1	components					2791:2800	self-produced and host-derived matrix components	2753:2800	self-produced and host-derived matrix components	2753:2800	The cell-cell aggregation of S. epidermidis can be activated by host factors without relying on either of the major adhesins, PIA and Embp, indicating a need to revisit the basic question of how S. epidermidis deploys self-produced and host-derived matrix components to form antibiotic-tolerant biofilms in vivo.
33492206	0	36	from	need	25:28	arg1	formation					129:137	Staphylococcus epidermidis biofilm formation	94:137	Staphylococcus epidermidis biofilm formation	94:137	Host factors abolish the need for polysaccharides and extracellular matrix-binding protein in Staphylococcus epidermidis biofilm formation.
33492206	8	37	theme	structural	1346:1355	arg1	differences					1357:1367	structural differences	1346:1367	structural differences caused by the different matrix components and growth conditions	1346:1431	The amount, structure, elasticity and antimicrobial penetration of biofilms was quantified to describe structural differences caused by the different matrix components and growth conditions.
33492206	16	38	theme	human	2510:2514	arg1	components					2523:2532	human matrix components	2510:2532	human matrix components	2510:2532	We suggest that the assumed importance of polysaccharides for biofilm formation is an artefact from studying biofilms in laboratory media void of human matrix components.
33492206	0	39	theme	Host	0:3	arg1	factors					5:11	Host factors	0:11	Host factors	0:11	Host factors abolish the need for polysaccharides and extracellular matrix-binding protein in Staphylococcus epidermidis biofilm formation.
33492206	16	40	theme	assumed	2384:2390	arg1	importance					2392:2401	the assumed importance	2380:2401	the assumed importance of polysaccharides for biofilm formation	2380:2442	We suggest that the assumed importance of polysaccharides for biofilm formation is an artefact from studying biofilms in laboratory media void of human matrix components.
33492206	16	40	theme	assumed	2384:2390	arg1	artefact					2450:2457	an artefact	2447:2457	an artefact from studying biofilms in laboratory media void of human matrix components	2447:2532	We suggest that the assumed importance of polysaccharides for biofilm formation is an artefact from studying biofilms in laboratory media void of human matrix components.
33492206	5	41	theme	adhesins	571:578	arg1	regulation					542:551	the dichotomic regulation	527:551	the dichotomic regulation of S. epidermidis adhesins	527:578	We hypothesize that the dichotomic regulation of S. epidermidis adhesins is linked to whether it is inside a host or not, and that in vitro biofilm investigations in laboratory media may not reflect actual biofilms in vivo.Aim.
33492206	3	42	theme	extracellular	437:449	arg1	Embp					475:478	Embp	475:478	Embp	475:478	It can deploy several strategies for biofilm development using either polysaccharide intercellular adhesin (PIA), extracellular DNA (eDNA) and/or proteins, such as the extracellular matrix-binding protein (Embp).
33492206	3	42	theme	extracellular	437:449	arg1	protein					466:472	the extracellular matrix-binding protein	433:472	the extracellular matrix-binding protein (Embp)	433:479	It can deploy several strategies for biofilm development using either polysaccharide intercellular adhesin (PIA), extracellular DNA (eDNA) and/or proteins, such as the extracellular matrix-binding protein (Embp).
33492206	2	43	theme	due	229:231	arg1	infections					218:227	implant-associated infections	199:227	implant-associated infections due to its capability to form biofilms	199:266	Staphylococcus epidermidis is predominant in implant-associated infections due to its capability to form biofilms.
33492206	17	44	theme	antibiotic-tolerant	2810:2828	arg1	biofilms					2830:2837	antibiotic-tolerant biofilms	2810:2837	antibiotic-tolerant biofilms	2810:2837	The cell-cell aggregation of S. epidermidis can be activated by host factors without relying on either of the major adhesins, PIA and Embp, indicating a need to revisit the basic question of how S. epidermidis deploys self-produced and host-derived matrix components to form antibiotic-tolerant biofilms in vivo.
33492206	6	45	theme	different	854:862	arg1	roles					864:868	different roles	854:868	different roles	854:868	We address the importance of PIA and Embp in biofilm grown in 'humanized' media to understand if these components play different roles in biofilm formation under conditions where bacteria can incorporate host proteins in the biofilm matrix.Methodology.
33492206	5	46	dep	in	638:639	arg1	vitro					641:645	vitro	641:645	vitro	641:645	We hypothesize that the dichotomic regulation of S. epidermidis adhesins is linked to whether it is inside a host or not, and that in vitro biofilm investigations in laboratory media may not reflect actual biofilms in vivo.Aim.
33492206	7	47	from	promotor	1112:1119	arg1	embp					1089:1092	embp	1089:1092	embp from an inducible promotor	1089:1119	S. epidermidis 1585 WT (deficient in icaADBC), and derivative strains that either lack embp, express embp from an inducible promotor, or express icaADBC from a plasmid, were cultivated in standard laboratory media, or in media with human plasma or serum.
33492206	2	48	theme	implant-associated	199:216	arg1	infections					218:227	implant-associated infections	199:227	implant-associated infections due to its capability to form biofilms	199:266	Staphylococcus epidermidis is predominant in implant-associated infections due to its capability to form biofilms.
33492206	8	49	theme	matrix	1393:1398	arg1	components					1400:1409	the different matrix components	1379:1409	the different matrix components	1379:1409	The amount, structure, elasticity and antimicrobial penetration of biofilms was quantified to describe structural differences caused by the different matrix components and growth conditions.
33492206	6	50	theme	Embp	772:775	arg1	importance					750:759	the importance	746:759	the importance of PIA and Embp in biofilm grown in 'humanized' media to understand if these components play different roles in biofilm formation under conditions where bacteria can incorporate host proteins in the biofilm matrix.Methodology	746:985	We address the importance of PIA and Embp in biofilm grown in 'humanized' media to understand if these components play different roles in biofilm formation under conditions where bacteria can incorporate host proteins in the biofilm matrix.Methodology.
33492206	6	51	theme	PIA	764:766	arg1	importance					750:759	the importance	746:759	the importance of PIA and Embp in biofilm grown in 'humanized' media to understand if these components play different roles in biofilm formation under conditions where bacteria can incorporate host proteins in the biofilm matrix.Methodology	746:985	We address the importance of PIA and Embp in biofilm grown in 'humanized' media to understand if these components play different roles in biofilm formation under conditions where bacteria can incorporate host proteins in the biofilm matrix.Methodology.
33492206	16	52	theme	components	2523:2532	arg1	void					2502:2505	void	2502:2505	void	2502:2505	We suggest that the assumed importance of polysaccharides for biofilm formation is an artefact from studying biofilms in laboratory media void of human matrix components.
33492206	11	53	theme	plasma	1864:1869	arg1	presence					1846:1853	the presence	1842:1853	the presence of human plasma	1842:1869	However, these observations were not representative of the biofilm phenotype in the presence of human plasma.
33492206	6	54	from	importance	750:759	arg1	biofilm					780:786	biofilm	780:786	biofilm grown in 'humanized' media to understand if these components play different roles in biofilm formation under conditions where bacteria can incorporate host proteins in the biofilm matrix.Methodology	780:985	We address the importance of PIA and Embp in biofilm grown in 'humanized' media to understand if these components play different roles in biofilm formation under conditions where bacteria can incorporate host proteins in the biofilm matrix.Methodology.
33492206	7	55	theme	inducible	1102:1110	arg1	promotor					1112:1119	an inducible promotor	1099:1119	an inducible promotor	1099:1119	S. epidermidis 1585 WT (deficient in icaADBC), and derivative strains that either lack embp, express embp from an inducible promotor, or express icaADBC from a plasmid, were cultivated in standard laboratory media, or in media with human plasma or serum.
33492206	16	56	theme	matrix	2516:2521	arg1	components					2523:2532	human matrix components	2510:2532	human matrix components	2510:2532	We suggest that the assumed importance of polysaccharides for biofilm formation is an artefact from studying biofilms in laboratory media void of human matrix components.
33492206	5	57	theme	in	638:639	arg1	investigations					655:668	in vitro biofilm investigations	638:668	in vitro biofilm investigations in laboratory media	638:688	We hypothesize that the dichotomic regulation of S. epidermidis adhesins is linked to whether it is inside a host or not, and that in vitro biofilm investigations in laboratory media may not reflect actual biofilms in vivo.Aim.
33492206	6	58	theme	humanized	798:806	arg1	media					809:813	'humanized' media	797:813	'humanized' media to understand if these components play different roles in biofilm formation under conditions where bacteria can incorporate host proteins in the biofilm matrix.Methodology	797:985	We address the importance of PIA and Embp in biofilm grown in 'humanized' media to understand if these components play different roles in biofilm formation under conditions where bacteria can incorporate host proteins in the biofilm matrix.Methodology.
33492206	10	59	theme	laboratory	1744:1753	arg1	media					1755:1759	laboratory media	1744:1759	laboratory media	1744:1759	S. epidermidis 1585 required polysaccharides to form biofilm in laboratory media.
33492206	5	60	theme	biofilm	647:653	arg1	investigations					655:668	in vitro biofilm investigations	638:668	in vitro biofilm investigations in laboratory media	638:688	We hypothesize that the dichotomic regulation of S. epidermidis adhesins is linked to whether it is inside a host or not, and that in vitro biofilm investigations in laboratory media may not reflect actual biofilms in vivo.Aim.
33492206	5	61	theme	S.	556:557	arg1	adhesins					571:578	S. epidermidis adhesins	556:578	S. epidermidis adhesins	556:578	We hypothesize that the dichotomic regulation of S. epidermidis adhesins is linked to whether it is inside a host or not, and that in vitro biofilm investigations in laboratory media may not reflect actual biofilms in vivo.Aim.
33492206	15	62	from	differences	2296:2306	arg1	penetration					2322:2332	vancomycin penetration	2311:2332	vancomycin penetration	2311:2332	Despite stark differences in the amount and composition of biofilms formed by polysaccharide-producing and Embp-producing strains in different media, there were no differences in vancomycin penetration or susceptibility.Conclusion.
33492206	15	62	from	differences	2296:2306	arg1	susceptibility.Conclusion					2337:2361	susceptibility.Conclusion	2337:2361	susceptibility.Conclusion	2337:2361	Despite stark differences in the amount and composition of biofilms formed by polysaccharide-producing and Embp-producing strains in different media, there were no differences in vancomycin penetration or susceptibility.Conclusion.
33492206	10	63	from	biofilm	1733:1739	arg1	media					1755:1759	laboratory media	1744:1759	laboratory media	1744:1759	S. epidermidis 1585 required polysaccharides to form biofilm in laboratory media.
33492206	7	64	from	deficient	1012:1020	arg1	icaADBC					1025:1031	icaADBC	1025:1031	icaADBC	1025:1031	S. epidermidis 1585 WT (deficient in icaADBC), and derivative strains that either lack embp, express embp from an inducible promotor, or express icaADBC from a plasmid, were cultivated in standard laboratory media, or in media with human plasma or serum.
33492206	3	65	theme	several	283:289	arg1	strategies					291:300	several strategies	283:300	several strategies for biofilm development	283:324	It can deploy several strategies for biofilm development using either polysaccharide intercellular adhesin (PIA), extracellular DNA (eDNA) and/or proteins, such as the extracellular matrix-binding protein (Embp).
33492206	15	66	theme	biofilms	2191:2198	arg1	amount					2165:2170	amount	2165:2170	amount	2165:2170	Despite stark differences in the amount and composition of biofilms formed by polysaccharide-producing and Embp-producing strains in different media, there were no differences in vancomycin penetration or susceptibility.Conclusion.
33492206	15	66	theme	biofilms	2191:2198	arg1	biofilms					2191:2198	biofilms	2191:2198	biofilms formed by polysaccharide-producing and Embp-producing strains in different media	2191:2279	Despite stark differences in the amount and composition of biofilms formed by polysaccharide-producing and Embp-producing strains in different media, there were no differences in vancomycin penetration or susceptibility.Conclusion.
33492206	15	66	theme	biofilms	2191:2198	arg1	composition					2176:2186	composition	2176:2186	composition	2176:2186	Despite stark differences in the amount and composition of biofilms formed by polysaccharide-producing and Embp-producing strains in different media, there were no differences in vancomycin penetration or susceptibility.Conclusion.
33492206	7	67	from	icaADBC	1025:1031	arg1	deficient					1012:1020	deficient	1012:1020	deficient	1012:1020	S. epidermidis 1585 WT (deficient in icaADBC), and derivative strains that either lack embp, express embp from an inducible promotor, or express icaADBC from a plasmid, were cultivated in standard laboratory media, or in media with human plasma or serum.
33492206	15	68	dep	amount	2165:2170	arg1	the					2161:2163	the	2161:2163	the	2161:2163	Despite stark differences in the amount and composition of biofilms formed by polysaccharide-producing and Embp-producing strains in different media, there were no differences in vancomycin penetration or susceptibility.Conclusion.
33492206	9	69	dep	environment	1605:1615	arg1	response					1584:1591	response	1584:1591	response	1584:1591	Finally, we quantified the initiation of biofilms as suspended aggregates in response to host factors to determine how quickly the cells aggregate in response to the host environment and reach a size that protects them from phagocytosis.Results.
33492206	9	70	theme	biofilms	1475:1482	arg1	aggregates					1497:1506	suspended aggregates	1487:1506	suspended aggregates	1487:1506	Finally, we quantified the initiation of biofilms as suspended aggregates in response to host factors to determine how quickly the cells aggregate in response to the host environment and reach a size that protects them from phagocytosis.Results.
33492206	9	70	theme	biofilms	1475:1482	arg1	initiation					1461:1470	the initiation	1457:1470	the initiation of biofilms	1457:1482	Finally, we quantified the initiation of biofilms as suspended aggregates in response to host factors to determine how quickly the cells aggregate in response to the host environment and reach a size that protects them from phagocytosis.Results.
33492206	12	71	theme	biofilm	1946:1952	arg1	formation					1954:1962	biofilm formation	1946:1962	biofilm formation	1946:1962	If human plasma were present, polysaccharides and Embp were redundant for biofilm formation.
33492206	15	72	from	differences	2146:2156	arg1	amount					2165:2170	amount	2165:2170	amount	2165:2170	Despite stark differences in the amount and composition of biofilms formed by polysaccharide-producing and Embp-producing strains in different media, there were no differences in vancomycin penetration or susceptibility.Conclusion.
33492206	15	72	from	differences	2146:2156	arg1	biofilms					2191:2198	biofilms	2191:2198	biofilms formed by polysaccharide-producing and Embp-producing strains in different media	2191:2279	Despite stark differences in the amount and composition of biofilms formed by polysaccharide-producing and Embp-producing strains in different media, there were no differences in vancomycin penetration or susceptibility.Conclusion.
33492206	15	72	from	differences	2146:2156	arg1	composition					2176:2186	composition	2176:2186	composition	2176:2186	Despite stark differences in the amount and composition of biofilms formed by polysaccharide-producing and Embp-producing strains in different media, there were no differences in vancomycin penetration or susceptibility.Conclusion.
33492206	11	73	theme	biofilm	1821:1827	arg1	phenotype					1829:1837	the biofilm phenotype	1817:1837	the biofilm phenotype in the presence of human plasma	1817:1869	However, these observations were not representative of the biofilm phenotype in the presence of human plasma.
33492206	5	74	theme	actual	706:711	arg1	biofilms					713:720	actual biofilms	706:720	actual biofilms	706:720	We hypothesize that the dichotomic regulation of S. epidermidis adhesins is linked to whether it is inside a host or not, and that in vitro biofilm investigations in laboratory media may not reflect actual biofilms in vivo.Aim.
33492206	15	75	theme	polysaccharide-producing	2210:2233	arg1	strains					2254:2260	polysaccharide-producing and Embp-producing strains	2210:2260	polysaccharide-producing and Embp-producing strains	2210:2260	Despite stark differences in the amount and composition of biofilms formed by polysaccharide-producing and Embp-producing strains in different media, there were no differences in vancomycin penetration or susceptibility.Conclusion.
33492206	3	76	theme	intercellular	354:366	arg1	PIA					377:379	PIA	377:379	PIA	377:379	It can deploy several strategies for biofilm development using either polysaccharide intercellular adhesin (PIA), extracellular DNA (eDNA) and/or proteins, such as the extracellular matrix-binding protein (Embp).
33492206	3	76	theme	intercellular	354:366	arg1	adhesin					368:374	either polysaccharide intercellular adhesin	332:374	either polysaccharide intercellular adhesin (PIA)	332:380	It can deploy several strategies for biofilm development using either polysaccharide intercellular adhesin (PIA), extracellular DNA (eDNA) and/or proteins, such as the extracellular matrix-binding protein (Embp).
33492206	0	77	theme	matrix-binding	68:81	arg1	protein					83:89	extracellular matrix-binding protein	54:89	extracellular matrix-binding protein	54:89	Host factors abolish the need for polysaccharides and extracellular matrix-binding protein in Staphylococcus epidermidis biofilm formation.
33492206	7	78	theme	deficient	1012:1020	arg1	1585 WT					1003:1009	S. epidermidis 1585 WT	988:1009	S. epidermidis 1585 WT (deficient in icaADBC)	988:1032	S. epidermidis 1585 WT (deficient in icaADBC), and derivative strains that either lack embp, express embp from an inducible promotor, or express icaADBC from a plasmid, were cultivated in standard laboratory media, or in media with human plasma or serum.
33492206	3	79	theme	extracellular	383:395	arg1	eDNA					402:405	eDNA	402:405	eDNA	402:405	It can deploy several strategies for biofilm development using either polysaccharide intercellular adhesin (PIA), extracellular DNA (eDNA) and/or proteins, such as the extracellular matrix-binding protein (Embp).
33492206	3	79	theme	extracellular	383:395	arg1	DNA					397:399	extracellular DNA	383:399	extracellular DNA (eDNA)	383:406	It can deploy several strategies for biofilm development using either polysaccharide intercellular adhesin (PIA), extracellular DNA (eDNA) and/or proteins, such as the extracellular matrix-binding protein (Embp).
33492206	7	80	with	media	1209:1213	arg1	plasma					1226:1231	plasma	1226:1231	plasma	1226:1231	S. epidermidis 1585 WT (deficient in icaADBC), and derivative strains that either lack embp, express embp from an inducible promotor, or express icaADBC from a plasmid, were cultivated in standard laboratory media, or in media with human plasma or serum.
33492206	7	80	with	media	1209:1213	arg1	serum					1236:1240	serum	1236:1240	serum	1236:1240	S. epidermidis 1585 WT (deficient in icaADBC), and derivative strains that either lack embp, express embp from an inducible promotor, or express icaADBC from a plasmid, were cultivated in standard laboratory media, or in media with human plasma or serum.
33492206	7	81	theme	laboratory	1185:1194	arg1	media					1196:1200	standard laboratory media	1176:1200	standard laboratory media	1176:1200	S. epidermidis 1585 WT (deficient in icaADBC), and derivative strains that either lack embp, express embp from an inducible promotor, or express icaADBC from a plasmid, were cultivated in standard laboratory media, or in media with human plasma or serum.
33492206	7	82	theme	S.	988:989	arg1	1585 WT					1003:1009	S. epidermidis 1585 WT	988:1009	S. epidermidis 1585 WT (deficient in icaADBC)	988:1032	S. epidermidis 1585 WT (deficient in icaADBC), and derivative strains that either lack embp, express embp from an inducible promotor, or express icaADBC from a plasmid, were cultivated in standard laboratory media, or in media with human plasma or serum.
33492206	8	83	dep	amount	1247:1252	arg1	The					1243:1245	The	1243:1245	The	1243:1245	The amount, structure, elasticity and antimicrobial penetration of biofilms was quantified to describe structural differences caused by the different matrix components and growth conditions.
33492206	7	84	from	plasmid	1148:1154	arg1	icaADBC					1133:1139	icaADBC	1133:1139	icaADBC from a plasmid	1133:1154	S. epidermidis 1585 WT (deficient in icaADBC), and derivative strains that either lack embp, express embp from an inducible promotor, or express icaADBC from a plasmid, were cultivated in standard laboratory media, or in media with human plasma or serum.
33492206	17	85	theme	host	2599:2602	arg1	factors					2604:2610	host factors	2599:2610	host factors	2599:2610	The cell-cell aggregation of S. epidermidis can be activated by host factors without relying on either of the major adhesins, PIA and Embp, indicating a need to revisit the basic question of how S. epidermidis deploys self-produced and host-derived matrix components to form antibiotic-tolerant biofilms in vivo.
33492206	8	86	theme	biofilms	1310:1317	arg1	biofilms					1310:1317	biofilms	1310:1317	biofilms	1310:1317	The amount, structure, elasticity and antimicrobial penetration of biofilms was quantified to describe structural differences caused by the different matrix components and growth conditions.
33492206	8	86	theme	biofilms	1310:1317	arg1	elasticity					1266:1275	elasticity	1266:1275	elasticity	1266:1275	The amount, structure, elasticity and antimicrobial penetration of biofilms was quantified to describe structural differences caused by the different matrix components and growth conditions.
33492206	8	86	theme	biofilms	1310:1317	arg1	penetration					1295:1305	antimicrobial penetration	1281:1305	antimicrobial penetration	1281:1305	The amount, structure, elasticity and antimicrobial penetration of biofilms was quantified to describe structural differences caused by the different matrix components and growth conditions.
33492206	8	86	theme	biofilms	1310:1317	arg1	structure					1255:1263	structure	1255:1263	structure	1255:1263	The amount, structure, elasticity and antimicrobial penetration of biofilms was quantified to describe structural differences caused by the different matrix components and growth conditions.
33492206	8	86	theme	biofilms	1310:1317	arg1	amount					1247:1252	amount	1247:1252	amount	1247:1252	The amount, structure, elasticity and antimicrobial penetration of biofilms was quantified to describe structural differences caused by the different matrix components and growth conditions.
33492206	13	87	theme	suspended	2041:2049	arg1	aggregates					2051:2060	suspended aggregates	2041:2060	suspended aggregates	2041:2060	Biofilms formed in human plasma were loosely attached and existed mostly as suspended aggregates.
33492206	17	88	theme	matrix	2784:2789	arg1	components					2791:2800	self-produced and host-derived matrix components	2753:2800	self-produced and host-derived matrix components	2753:2800	The cell-cell aggregation of S. epidermidis can be activated by host factors without relying on either of the major adhesins, PIA and Embp, indicating a need to revisit the basic question of how S. epidermidis deploys self-produced and host-derived matrix components to form antibiotic-tolerant biofilms in vivo.
33492206	5	89	from	investigations	655:668	arg1	media					684:688	laboratory media	673:688	laboratory media	673:688	We hypothesize that the dichotomic regulation of S. epidermidis adhesins is linked to whether it is inside a host or not, and that in vitro biofilm investigations in laboratory media may not reflect actual biofilms in vivo.Aim.
33492206	12	90	theme	human	1875:1879	arg1	plasma					1881:1886	human plasma	1875:1886	human plasma	1875:1886	If human plasma were present, polysaccharides and Embp were redundant for biofilm formation.
33492206	3	91	theme	matrix-binding	451:464	arg1	Embp					475:478	Embp	475:478	Embp	475:478	It can deploy several strategies for biofilm development using either polysaccharide intercellular adhesin (PIA), extracellular DNA (eDNA) and/or proteins, such as the extracellular matrix-binding protein (Embp).
33492206	3	91	theme	matrix-binding	451:464	arg1	protein					466:472	the extracellular matrix-binding protein	433:472	the extracellular matrix-binding protein (Embp)	433:479	It can deploy several strategies for biofilm development using either polysaccharide intercellular adhesin (PIA), extracellular DNA (eDNA) and/or proteins, such as the extracellular matrix-binding protein (Embp).
33492206	7	92	theme	standard	1176:1183	arg1	media					1196:1200	standard laboratory media	1176:1200	standard laboratory media	1176:1200	S. epidermidis 1585 WT (deficient in icaADBC), and derivative strains that either lack embp, express embp from an inducible promotor, or express icaADBC from a plasmid, were cultivated in standard laboratory media, or in media with human plasma or serum.
33492206	2	93	attach	predominant	184:194	arg2	epidermidis					169:179	Staphylococcus epidermidis	154:179	Staphylococcus epidermidis	154:179	Staphylococcus epidermidis is predominant in implant-associated infections due to its capability to form biofilms.
33492206	2	93	attach	predominant	184:194	arg1	infections					218:227	implant-associated infections	199:227	implant-associated infections due to its capability to form biofilms	199:266	Staphylococcus epidermidis is predominant in implant-associated infections due to its capability to form biofilms.
33492206	8	94	theme	different	1383:1391	arg1	components					1400:1409	the different matrix components	1379:1409	the different matrix components	1379:1409	The amount, structure, elasticity and antimicrobial penetration of biofilms was quantified to describe structural differences caused by the different matrix components and growth conditions.
33492206	11	95	theme	human	1858:1862	arg1	plasma					1864:1869	human plasma	1858:1869	human plasma	1858:1869	However, these observations were not representative of the biofilm phenotype in the presence of human plasma.
33492206	15	96	theme	different	2265:2273	arg1	media					2275:2279	different media	2265:2279	different media	2265:2279	Despite stark differences in the amount and composition of biofilms formed by polysaccharide-producing and Embp-producing strains in different media, there were no differences in vancomycin penetration or susceptibility.Conclusion.
33492206	9	97	theme	host	1600:1603	arg1	environment					1605:1615	the host environment	1596:1615	the host environment	1596:1615	Finally, we quantified the initiation of biofilms as suspended aggregates in response to host factors to determine how quickly the cells aggregate in response to the host environment and reach a size that protects them from phagocytosis.Results.
33492206	17	98	theme	cell-cell	2539:2547	arg1	aggregation					2549:2559	The cell-cell aggregation	2535:2559	The cell-cell aggregation of S. epidermidis	2535:2577	The cell-cell aggregation of S. epidermidis can be activated by host factors without relying on either of the major adhesins, PIA and Embp, indicating a need to revisit the basic question of how S. epidermidis deploys self-produced and host-derived matrix components to form antibiotic-tolerant biofilms in vivo.
33492206	13	99	theme	human	1984:1988	arg1	plasma					1990:1995	human plasma	1984:1995	human plasma	1984:1995	Biofilms formed in human plasma were loosely attached and existed mostly as suspended aggregates.
33492206	2	100	from	infections	218:227	arg1	predominant					184:194	predominant	184:194	predominant	184:194	Staphylococcus epidermidis is predominant in implant-associated infections due to its capability to form biofilms.
33492206	7	101	dep	lack	1070:1073	arg1	either					1063:1068	either	1063:1068	either	1063:1068	S. epidermidis 1585 WT (deficient in icaADBC), and derivative strains that either lack embp, express embp from an inducible promotor, or express icaADBC from a plasmid, were cultivated in standard laboratory media, or in media with human plasma or serum.
33492206	8	102	theme	growth	1415:1420	arg1	conditions					1422:1431	growth conditions	1415:1431	growth conditions	1415:1431	The amount, structure, elasticity and antimicrobial penetration of biofilms was quantified to describe structural differences caused by the different matrix components and growth conditions.
32126203	0	0	theme	adsorption	72:81	arg1	performance					83:93	cationic and anionic dye adsorption performance	47:93	cationic and anionic dye adsorption performance of functionalized silica immobilized chitosan bio-polymer	47:151	Synthesis, characterization, and assessment of cationic and anionic dye adsorption performance of functionalized silica immobilized chitosan bio-polymer.
32126203	9	1	theme	beads	1406:1410	arg1	capacity					1385:1392	the capacity	1381:1392	the capacity of chitosan beads	1381:1410	The adsorbed yield of MB using the composite beads increased three times more than the capacity of chitosan beads and it was improved 1.4 times in the case of AB25.
32126203	5	2	theme	gel	760:762	arg1	beads					764:768	the prepared composite gel beads	737:768	the prepared composite gel beads	737:768	The morphology of the prepared composite gel beads exhibited a spherical shape surface covered by silica particles.
32126203	10	3	theme	chemi-sorption	1575:1588	arg1	nature					1590:1595	the chemi-sorption nature	1571:1595	the chemi-sorption nature of the adsorption phenomenon	1571:1624	The mean free energy values (74.53-223.61 kJ mol-1), computed from the Dubinin-Radushkevich model suggested the chemi-sorption nature of the adsorption phenomenon.
32126203	9	4	theme	more	1371:1374	arg1	times					1365:1369	three times	1359:1369	three times more than the capacity of chitosan beads	1359:1410	The adsorbed yield of MB using the composite beads increased three times more than the capacity of chitosan beads and it was improved 1.4 times in the case of AB25.
32126203	0	5	theme	dye	68:70	arg1	performance					83:93	cationic and anionic dye adsorption performance	47:93	cationic and anionic dye adsorption performance of functionalized silica immobilized chitosan bio-polymer	47:151	Synthesis, characterization, and assessment of cationic and anionic dye adsorption performance of functionalized silica immobilized chitosan bio-polymer.
32126203	5	6	theme	beads	764:768	arg1	morphology					723:732	The morphology	719:732	The morphology of the prepared composite gel beads	719:768	The morphology of the prepared composite gel beads exhibited a spherical shape surface covered by silica particles.
32126203	1	7	theme	polymers	179:186	arg1	functionalization					158:174	The functionalization	154:174	The functionalization of polymers	154:186	The functionalization of polymers is still an efficient scheme to provide materials with new properties.
32126203	1	7	theme	polymers	179:186	arg1	scheme					210:215	an efficient scheme	197:215	an efficient scheme to provide materials with new properties	197:256	The functionalization of polymers is still an efficient scheme to provide materials with new properties.
32126203	9	8	theme	AB25	1457:1460	arg1	case					1449:1452	the case	1445:1452	the case of AB25	1445:1460	The adsorbed yield of MB using the composite beads increased three times more than the capacity of chitosan beads and it was improved 1.4 times in the case of AB25.
32126203	4	9	theme	FT-IR	590:594	arg1	investigation					596:608	FT-IR investigation	590:608	FT-IR investigation	590:608	FT-IR investigation suggested that the interaction between chitosan and functionalized silica occurred through hydrogen bonding.
32126203	2	10	theme	spherical	407:415	arg1	beads					417:421	chitosan bio-polymer spherical beads	386:421	chitosan bio-polymer spherical beads	386:421	In this paper, 4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica was successfully immobilized onto chitosan bio-polymer spherical beads to improve their adsorption characteristics.
32126203	8	11	theme	adsorption	1122:1131	arg1	dyes					1143:1146	dyes	1143:1146	dyes	1143:1146	The results showed that the highest adsorption amount of dyes was reached using the functionalized chitosan beads under the following conditions; pH = 5 for AB25 and pH = 6 for MB, time = 120 min, and T = 20 °C.
32126203	8	11	theme	adsorption	1122:1131	arg1	amount					1133:1138	the highest adsorption amount	1110:1138	the highest adsorption amount of dyes	1110:1146	The results showed that the highest adsorption amount of dyes was reached using the functionalized chitosan beads under the following conditions; pH = 5 for AB25 and pH = 6 for MB, time = 120 min, and T = 20 °C.
32126203	4	12	theme	hydrogen	701:708	arg1	bonding					710:716	hydrogen bonding	701:716	hydrogen bonding	701:716	FT-IR investigation suggested that the interaction between chitosan and functionalized silica occurred through hydrogen bonding.
32126203	0	13	theme	performance	83:93	arg1	characterization					11:26	characterization	11:26	characterization	11:26	Synthesis, characterization, and assessment of cationic and anionic dye adsorption performance of functionalized silica immobilized chitosan bio-polymer.
32126203	0	13	theme	performance	83:93	arg1	assessment					33:42	assessment	33:42	assessment	33:42	Synthesis, characterization, and assessment of cationic and anionic dye adsorption performance of functionalized silica immobilized chitosan bio-polymer.
32126203	0	13	theme	performance	83:93	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis, characterization, and assessment of cationic and anionic dye adsorption performance of functionalized silica immobilized chitosan bio-polymer.
32126203	5	14	theme	silica	817:822	arg1	particles					824:832	silica particles	817:832	silica particles	817:832	The morphology of the prepared composite gel beads exhibited a spherical shape surface covered by silica particles.
32126203	8	15	theme	highest	1114:1120	arg1	dyes					1143:1146	dyes	1143:1146	dyes	1143:1146	The results showed that the highest adsorption amount of dyes was reached using the functionalized chitosan beads under the following conditions; pH = 5 for AB25 and pH = 6 for MB, time = 120 min, and T = 20 °C.
32126203	8	15	theme	highest	1114:1120	arg1	amount					1133:1138	the highest adsorption amount	1110:1138	the highest adsorption amount of dyes	1110:1146	The results showed that the highest adsorption amount of dyes was reached using the functionalized chitosan beads under the following conditions; pH = 5 for AB25 and pH = 6 for MB, time = 120 min, and T = 20 °C.
32126203	8	16	dep	showed	1098:1103	arg1	pH = 5					1232:1237	pH = 5	1232:1237	pH = 5	1232:1237	The results showed that the highest adsorption amount of dyes was reached using the functionalized chitosan beads under the following conditions; pH = 5 for AB25 and pH = 6 for MB, time = 120 min, and T = 20 °C.
32126203	8	16	dep	showed	1098:1103	arg1	pH = 6					1252:1257	pH = 6	1252:1257	pH = 6	1252:1257	The results showed that the highest adsorption amount of dyes was reached using the functionalized chitosan beads under the following conditions; pH = 5 for AB25 and pH = 6 for MB, time = 120 min, and T = 20 °C.
32126203	7	17	theme	time	993:996	arg1	influence					976:984	The influence	972:984	The influence of pH, time, dye concentration, and temperature on the adsorption characteristics	972:1066	The influence of pH, time, dye concentration, and temperature on the adsorption characteristics was investigated.
32126203	0	18	theme	silica	113:118	arg1	bio-polymer					141:151	functionalized silica immobilized chitosan bio-polymer	98:151	functionalized silica immobilized chitosan bio-polymer	98:151	Synthesis, characterization, and assessment of cationic and anionic dye adsorption performance of functionalized silica immobilized chitosan bio-polymer.
32126203	10	19	theme	free	1472:1475	arg1	74.53-223.61 kJ mol-1					1492:1512	74.53-223.61 kJ mol-1	1492:1512	74.53-223.61 kJ mol-1	1492:1512	The mean free energy values (74.53-223.61 kJ mol-1), computed from the Dubinin-Radushkevich model suggested the chemi-sorption nature of the adsorption phenomenon.
32126203	10	19	theme	free	1472:1475	arg1	values					1484:1489	The mean free energy values	1463:1489	The mean free energy values (74.53-223.61 kJ mol-1)	1463:1513	The mean free energy values (74.53-223.61 kJ mol-1), computed from the Dubinin-Radushkevich model suggested the chemi-sorption nature of the adsorption phenomenon.
32126203	6	20	theme	blue	926:929	arg1	methylene					916:924	methylene blue	916:929	methylene blue (MB)	916:934	The unfunctionalized and functionalized beads were studied for the adsorption of methylene blue (MB) and Acid blue 25 (AB25) from water.
32126203	6	20	theme	blue	926:929	arg1	MB					932:933	MB	932:933	MB	932:933	The unfunctionalized and functionalized beads were studied for the adsorption of methylene blue (MB) and Acid blue 25 (AB25) from water.
32126203	10	21	theme	energy	1477:1482	arg1	74.53-223.61 kJ mol-1					1492:1512	74.53-223.61 kJ mol-1	1492:1512	74.53-223.61 kJ mol-1	1492:1512	The mean free energy values (74.53-223.61 kJ mol-1), computed from the Dubinin-Radushkevich model suggested the chemi-sorption nature of the adsorption phenomenon.
32126203	10	21	theme	energy	1477:1482	arg1	values					1484:1489	The mean free energy values	1463:1489	The mean free energy values (74.53-223.61 kJ mol-1)	1463:1513	The mean free energy values (74.53-223.61 kJ mol-1), computed from the Dubinin-Radushkevich model suggested the chemi-sorption nature of the adsorption phenomenon.
32126203	0	22	theme	functionalized	98:111	arg1	bio-polymer					141:151	functionalized silica immobilized chitosan bio-polymer	98:151	functionalized silica immobilized chitosan bio-polymer	98:151	Synthesis, characterization, and assessment of cationic and anionic dye adsorption performance of functionalized silica immobilized chitosan bio-polymer.
32126203	6	23	theme	methylene	916:924	arg1	adsorption					902:911	the adsorption	898:911	the adsorption of methylene blue (MB) and Acid blue 25 (AB25) from water	898:969	The unfunctionalized and functionalized beads were studied for the adsorption of methylene blue (MB) and Acid blue 25 (AB25) from water.
32126203	7	24	from	influence	976:984	arg1	characteristics					1052:1066	the adsorption characteristics	1037:1066	the adsorption characteristics	1037:1066	The influence of pH, time, dye concentration, and temperature on the adsorption characteristics was investigated.
32126203	3	25	theme	functionalized	512:525	arg1	silica					527:532	the functionalized silica	508:532	the functionalized silica	508:532	The interaction between the polymer and the functionalized silica was analyzed using FT-IR spectroscopy and SEM analysis.
32126203	9	26	theme	composite	1333:1341	arg1	beads					1343:1347	the composite beads	1329:1347	the composite beads	1329:1347	The adsorbed yield of MB using the composite beads increased three times more than the capacity of chitosan beads and it was improved 1.4 times in the case of AB25.
32126203	5	27	theme	prepared	741:748	arg1	beads					764:768	the prepared composite gel beads	737:768	the prepared composite gel beads	737:768	The morphology of the prepared composite gel beads exhibited a spherical shape surface covered by silica particles.
32126203	10	28	theme	adsorption	1604:1613	arg1	phenomenon					1615:1624	the adsorption phenomenon	1600:1624	the adsorption phenomenon	1600:1624	The mean free energy values (74.53-223.61 kJ mol-1), computed from the Dubinin-Radushkevich model suggested the chemi-sorption nature of the adsorption phenomenon.
32126203	8	29	theme	chitosan	1185:1192	arg1	beads					1194:1198	the functionalized chitosan beads	1166:1198	the functionalized chitosan beads under the following conditions	1166:1229	The results showed that the highest adsorption amount of dyes was reached using the functionalized chitosan beads under the following conditions; pH = 5 for AB25 and pH = 6 for MB, time = 120 min, and T = 20 °C.
32126203	2	30	theme	adsorption	440:449	arg1	characteristics					451:465	their adsorption characteristics	434:465	their adsorption characteristics	434:465	In this paper, 4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica was successfully immobilized onto chitosan bio-polymer spherical beads to improve their adsorption characteristics.
32126203	10	31	theme	mean	1467:1470	arg1	74.53-223.61 kJ mol-1					1492:1512	74.53-223.61 kJ mol-1	1492:1512	74.53-223.61 kJ mol-1	1492:1512	The mean free energy values (74.53-223.61 kJ mol-1), computed from the Dubinin-Radushkevich model suggested the chemi-sorption nature of the adsorption phenomenon.
32126203	10	31	theme	mean	1467:1470	arg1	values					1484:1489	The mean free energy values	1463:1489	The mean free energy values (74.53-223.61 kJ mol-1)	1463:1513	The mean free energy values (74.53-223.61 kJ mol-1), computed from the Dubinin-Radushkevich model suggested the chemi-sorption nature of the adsorption phenomenon.
32126203	5	32	theme	composite	750:758	arg1	beads					764:768	the prepared composite gel beads	737:768	the prepared composite gel beads	737:768	The morphology of the prepared composite gel beads exhibited a spherical shape surface covered by silica particles.
32126203	1	33	theme	efficient	200:208	arg1	functionalization					158:174	The functionalization	154:174	The functionalization of polymers	154:186	The functionalization of polymers is still an efficient scheme to provide materials with new properties.
32126203	1	33	theme	efficient	200:208	arg1	scheme					210:215	an efficient scheme	197:215	an efficient scheme to provide materials with new properties	197:256	The functionalization of polymers is still an efficient scheme to provide materials with new properties.
32126203	9	34	theme	chitosan	1397:1404	arg1	beads					1406:1410	chitosan beads	1397:1410	chitosan beads	1397:1410	The adsorbed yield of MB using the composite beads increased three times more than the capacity of chitosan beads and it was improved 1.4 times in the case of AB25.
32126203	0	35	theme	chitosan	132:139	arg1	bio-polymer					141:151	functionalized silica immobilized chitosan bio-polymer	98:151	functionalized silica immobilized chitosan bio-polymer	98:151	Synthesis, characterization, and assessment of cationic and anionic dye adsorption performance of functionalized silica immobilized chitosan bio-polymer.
32126203	7	36	theme	dye	999:1001	arg1	concentration					1003:1015	dye concentration	999:1015	dye concentration	999:1015	The influence of pH, time, dye concentration, and temperature on the adsorption characteristics was investigated.
32126203	4	37	theme	functionalized	662:675	arg1	silica					677:682	functionalized silica	662:682	functionalized silica	662:682	FT-IR investigation suggested that the interaction between chitosan and functionalized silica occurred through hydrogen bonding.
32126203	0	38	theme	immobilized	120:130	arg1	bio-polymer					141:151	functionalized silica immobilized chitosan bio-polymer	98:151	functionalized silica immobilized chitosan bio-polymer	98:151	Synthesis, characterization, and assessment of cationic and anionic dye adsorption performance of functionalized silica immobilized chitosan bio-polymer.
32126203	2	39	theme	-N-propylpentanamide-functionalized	302:336	arg1	ethoxy-silica					338:350	4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica	274:350	4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica	274:350	In this paper, 4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica was successfully immobilized onto chitosan bio-polymer spherical beads to improve their adsorption characteristics.
32126203	7	40	theme	temperature	1022:1032	arg1	influence					976:984	The influence	972:984	The influence of pH, time, dye concentration, and temperature on the adsorption characteristics	972:1066	The influence of pH, time, dye concentration, and temperature on the adsorption characteristics was investigated.
32126203	3	41	theme	SEM	576:578	arg1	analysis					580:587	SEM analysis	576:587	SEM analysis	576:587	The interaction between the polymer and the functionalized silica was analyzed using FT-IR spectroscopy and SEM analysis.
32126203	8	42	theme	functionalized	1170:1183	arg1	beads					1194:1198	the functionalized chitosan beads	1166:1198	the functionalized chitosan beads under the following conditions	1166:1229	The results showed that the highest adsorption amount of dyes was reached using the functionalized chitosan beads under the following conditions; pH = 5 for AB25 and pH = 6 for MB, time = 120 min, and T = 20 °C.
32126203	9	43	theme	adsorbed	1302:1309	arg1	yield					1311:1315	The adsorbed yield	1298:1315	The adsorbed yield of MB using the composite beads	1298:1347	The adsorbed yield of MB using the composite beads increased three times more than the capacity of chitosan beads and it was improved 1.4 times in the case of AB25.
32126203	1	44	with	materials	228:236	arg1	properties					247:256	new properties	243:256	new properties	243:256	The functionalization of polymers is still an efficient scheme to provide materials with new properties.
32126203	0	45	theme	bio-polymer	141:151	arg1	performance					83:93	cationic and anionic dye adsorption performance	47:93	cationic and anionic dye adsorption performance of functionalized silica immobilized chitosan bio-polymer	47:151	Synthesis, characterization, and assessment of cationic and anionic dye adsorption performance of functionalized silica immobilized chitosan bio-polymer.
32126203	6	46	theme	AB25	954:957	arg1	adsorption					902:911	the adsorption	898:911	the adsorption of methylene blue (MB) and Acid blue 25 (AB25) from water	898:969	The unfunctionalized and functionalized beads were studied for the adsorption of methylene blue (MB) and Acid blue 25 (AB25) from water.
32126203	10	47	theme	phenomenon	1615:1624	arg1	nature					1590:1595	the chemi-sorption nature	1571:1595	the chemi-sorption nature of the adsorption phenomenon	1571:1624	The mean free energy values (74.53-223.61 kJ mol-1), computed from the Dubinin-Radushkevich model suggested the chemi-sorption nature of the adsorption phenomenon.
32126203	0	48	theme	cationic	47:54	arg1	performance					83:93	cationic and anionic dye adsorption performance	47:93	cationic and anionic dye adsorption performance of functionalized silica immobilized chitosan bio-polymer	47:151	Synthesis, characterization, and assessment of cationic and anionic dye adsorption performance of functionalized silica immobilized chitosan bio-polymer.
32126203	5	49	theme	spherical	782:790	arg1	surface					798:804	a spherical shape surface	780:804	a spherical shape surface covered by silica particles	780:832	The morphology of the prepared composite gel beads exhibited a spherical shape surface covered by silica particles.
32126203	10	50	theme	Dubinin-Radushkevich	1534:1553	arg1	model					1555:1559	the Dubinin-Radushkevich model	1530:1559	the Dubinin-Radushkevich model	1530:1559	The mean free energy values (74.53-223.61 kJ mol-1), computed from the Dubinin-Radushkevich model suggested the chemi-sorption nature of the adsorption phenomenon.
32126203	2	51	theme	bio-polymer	395:405	arg1	beads					417:421	chitosan bio-polymer spherical beads	386:421	chitosan bio-polymer spherical beads	386:421	In this paper, 4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica was successfully immobilized onto chitosan bio-polymer spherical beads to improve their adsorption characteristics.
32126203	5	52	theme	shape	792:796	arg1	surface					798:804	a spherical shape surface	780:804	a spherical shape surface covered by silica particles	780:832	The morphology of the prepared composite gel beads exhibited a spherical shape surface covered by silica particles.
32126203	9	53	theme	MB	1320:1321	arg1	yield					1311:1315	The adsorbed yield	1298:1315	The adsorbed yield of MB using the composite beads	1298:1347	The adsorbed yield of MB using the composite beads increased three times more than the capacity of chitosan beads and it was improved 1.4 times in the case of AB25.
32126203	7	54	theme	concentration	1003:1015	arg1	influence					976:984	The influence	972:984	The influence of pH, time, dye concentration, and temperature on the adsorption characteristics	972:1066	The influence of pH, time, dye concentration, and temperature on the adsorption characteristics was investigated.
32126203	2	55	theme	chitosan	386:393	arg1	beads					417:421	chitosan bio-polymer spherical beads	386:421	chitosan bio-polymer spherical beads	386:421	In this paper, 4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica was successfully immobilized onto chitosan bio-polymer spherical beads to improve their adsorption characteristics.
32126203	6	56	theme	blue	945:948	arg1	AB25					954:957	Acid blue 25 (AB25)	940:958	Acid blue 25 (AB25)	940:958	The unfunctionalized and functionalized beads were studied for the adsorption of methylene blue (MB) and Acid blue 25 (AB25) from water.
32126203	3	57	theme	FT-IR	553:557	arg1	spectroscopy					559:570	FT-IR spectroscopy	553:570	FT-IR spectroscopy	553:570	The interaction between the polymer and the functionalized silica was analyzed using FT-IR spectroscopy and SEM analysis.
32126203	7	58	theme	adsorption	1041:1050	arg1	characteristics					1052:1066	the adsorption characteristics	1037:1066	the adsorption characteristics	1037:1066	The influence of pH, time, dye concentration, and temperature on the adsorption characteristics was investigated.
32126203	6	59	theme	Acid	940:943	arg1	AB25					954:957	Acid blue 25 (AB25)	940:958	Acid blue 25 (AB25)	940:958	The unfunctionalized and functionalized beads were studied for the adsorption of methylene blue (MB) and Acid blue 25 (AB25) from water.
32126203	6	60	theme	functionalized	860:873	arg1	beads					875:879	The unfunctionalized and functionalized beads	835:879	The unfunctionalized and functionalized beads	835:879	The unfunctionalized and functionalized beads were studied for the adsorption of methylene blue (MB) and Acid blue 25 (AB25) from water.
32126203	1	61	theme	new	243:245	arg1	properties					247:256	new properties	243:256	new properties	243:256	The functionalization of polymers is still an efficient scheme to provide materials with new properties.
32126203	0	62	theme	anionic	60:66	arg1	performance					83:93	cationic and anionic dye adsorption performance	47:93	cationic and anionic dye adsorption performance of functionalized silica immobilized chitosan bio-polymer	47:151	Synthesis, characterization, and assessment of cationic and anionic dye adsorption performance of functionalized silica immobilized chitosan bio-polymer.
32126203	8	63	theme	dyes	1143:1146	arg1	dyes					1143:1146	dyes	1143:1146	dyes	1143:1146	The results showed that the highest adsorption amount of dyes was reached using the functionalized chitosan beads under the following conditions; pH = 5 for AB25 and pH = 6 for MB, time = 120 min, and T = 20 °C.
32126203	8	63	theme	dyes	1143:1146	arg1	amount					1133:1138	the highest adsorption amount	1110:1138	the highest adsorption amount of dyes	1110:1146	The results showed that the highest adsorption amount of dyes was reached using the functionalized chitosan beads under the following conditions; pH = 5 for AB25 and pH = 6 for MB, time = 120 min, and T = 20 °C.
32126203	7	64	theme	pH	989:990	arg1	influence					976:984	The influence	972:984	The influence of pH, time, dye concentration, and temperature on the adsorption characteristics	972:1066	The influence of pH, time, dye concentration, and temperature on the adsorption characteristics was investigated.
32126203	8	65	theme	following	1210:1218	arg1	conditions					1220:1229	the following conditions	1206:1229	the following conditions	1206:1229	The results showed that the highest adsorption amount of dyes was reached using the functionalized chitosan beads under the following conditions; pH = 5 for AB25 and pH = 6 for MB, time = 120 min, and T = 20 °C.
32126203	6	66	theme	unfunctionalized	839:854	arg1	beads					875:879	The unfunctionalized and functionalized beads	835:879	The unfunctionalized and functionalized beads	835:879	The unfunctionalized and functionalized beads were studied for the adsorption of methylene blue (MB) and Acid blue 25 (AB25) from water.
32126203	6	67	from	water	965:969	arg1	adsorption					902:911	the adsorption	898:911	the adsorption of methylene blue (MB) and Acid blue 25 (AB25) from water	898:969	The unfunctionalized and functionalized beads were studied for the adsorption of methylene blue (MB) and Acid blue 25 (AB25) from water.
32505987	5	0	theme	propionate	825:834	arg1	yields					806:811	the highest yields	794:811	the highest yields of lactate, propionate, and butyrate	794:848	During in vitro fermentation with human fecal inocula, GMOS gave the highest yields of lactate, propionate, and butyrate after 48 h fermentation.
32505987	0	1	theme	effects	80:86	arg1	assessment					56:65	In-vitro assessment	47:65	Dietary galactosyl and mannosyl carbohydrates: In-vitro assessment of prebiotic effects.	0:87	Dietary galactosyl and mannosyl carbohydrates: In-vitro assessment of prebiotic effects.
32505987	1	2	theme	galacto-oligosaccharide	210:232	arg1	activities					103:112	The prebiotic activities	89:112	The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa)	89:246	The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa) were evaluated by in vitro fermentation.
32505987	4	3	theme	molecular	687:695	arg1	Mw					705:706	Mw	705:706	Mw	705:706	Moreover, SCFA production in the hydrolyzed guar gum groups was closely related to the varied molecular weight (Mw) of the hydrolysate.
32505987	4	3	theme	molecular	687:695	arg1	weight					697:702	the varied molecular weight	676:702	the varied molecular weight (Mw) of the hydrolysate	676:726	Moreover, SCFA production in the hydrolyzed guar gum groups was closely related to the varied molecular weight (Mw) of the hydrolysate.
32505987	1	4	dep	in	266:267	arg1	vitro					269:273	vitro	269:273	vitro	269:273	The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa) were evaluated by in vitro fermentation.
32505987	3	5	theme	Clostridium	503:513	arg1	butyricum					515:523	Clostridium butyricum	503:523	Clostridium butyricum	503:523	GOS and GMOS markedly promoted the growth of bifidobacteria and Clostridium butyricum, respectively, whereas MOS showed the strongest butyrogenic effect.
32505987	5	6	theme	48 h	856:859	arg1	fermentation					861:872	48 h fermentation	856:872	48 h fermentation	856:872	During in vitro fermentation with human fecal inocula, GMOS gave the highest yields of lactate, propionate, and butyrate after 48 h fermentation.
32505987	2	7	theme	tested	293:298	arg1	carbohydrates					300:312	The tested carbohydrates	289:312	The tested carbohydrates	289:312	The tested carbohydrates showed selective prebiotic effects on bacterial growth, short-chain fatty acid (SCFA)-production, and substrate consumption.
32505987	5	8	dep	in	736:737	arg1	vitro					739:743	vitro	739:743	vitro	739:743	During in vitro fermentation with human fecal inocula, GMOS gave the highest yields of lactate, propionate, and butyrate after 48 h fermentation.
32505987	2	9	theme	fatty	382:386	arg1	SCFA					394:397	SCFA	394:397	SCFA	394:397	The tested carbohydrates showed selective prebiotic effects on bacterial growth, short-chain fatty acid (SCFA)-production, and substrate consumption.
32505987	2	9	theme	fatty	382:386	arg1	acid					388:391	short-chain fatty acid	370:391	short-chain fatty acid (SCFA)-production	370:409	The tested carbohydrates showed selective prebiotic effects on bacterial growth, short-chain fatty acid (SCFA)-production, and substrate consumption.
32505987	4	10	theme	hydrolysate	716:726	arg1	Mw					705:706	Mw	705:706	Mw	705:706	Moreover, SCFA production in the hydrolyzed guar gum groups was closely related to the varied molecular weight (Mw) of the hydrolysate.
32505987	4	10	theme	hydrolysate	716:726	arg1	weight					697:702	the varied molecular weight	676:702	the varied molecular weight (Mw) of the hydrolysate	676:726	Moreover, SCFA production in the hydrolyzed guar gum groups was closely related to the varied molecular weight (Mw) of the hydrolysate.
32505987	7	11	theme	individualized	1043:1056	arg1	effects					1068:1074	individualized prebiotic effects	1043:1074	individualized prebiotic effects which are associated with their chemical structures including their glycoside composition and Mw	1043:1171	Overall, our results indicate that galactosyl and mannosyl carbohydrates have individualized prebiotic effects which are associated with their chemical structures including their glycoside composition and Mw.
32505987	6	12	theme	butyricum	910:918	arg1	application					884:894	Combined application	875:894	Combined application of MOS and C. butyricum	875:918	Combined application of MOS and C. butyricum increased the abundance of Clostridiaceae_1.
32505987	7	13	theme	prebiotic	1058:1066	arg1	effects					1068:1074	individualized prebiotic effects	1043:1074	individualized prebiotic effects which are associated with their chemical structures including their glycoside composition and Mw	1043:1171	Overall, our results indicate that galactosyl and mannosyl carbohydrates have individualized prebiotic effects which are associated with their chemical structures including their glycoside composition and Mw.
32505987	3	14	theme	bifidobacteria	484:497	arg1	growth					474:479	the growth	470:479	the growth of bifidobacteria and Clostridium butyricum	470:523	GOS and GMOS markedly promoted the growth of bifidobacteria and Clostridium butyricum, respectively, whereas MOS showed the strongest butyrogenic effect.
32505987	2	15	theme	bacterial	352:360	arg1	growth					362:367	bacterial growth	352:367	bacterial growth	352:367	The tested carbohydrates showed selective prebiotic effects on bacterial growth, short-chain fatty acid (SCFA)-production, and substrate consumption.
32505987	7	16	theme	glycoside	1144:1152	arg1	composition					1154:1164	their glycoside composition	1138:1164	their glycoside composition	1138:1164	Overall, our results indicate that galactosyl and mannosyl carbohydrates have individualized prebiotic effects which are associated with their chemical structures including their glycoside composition and Mw.
32505987	1	17	dep	<1 kDa	144:149	arg1	1-10 kDa					158:165	1-10 kDa	158:165	1-10 kDa	158:165	The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa) were evaluated by in vitro fermentation.
32505987	1	17	dep	<1 kDa	144:149	arg1	GMPS					152:155	GMPS	152:155	GMPS	152:155	The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa) were evaluated by in vitro fermentation.
32505987	4	18	theme	guar	637:640	arg1	gum					642:644	the hydrolyzed guar gum	622:644	the hydrolyzed guar gum groups	622:651	Moreover, SCFA production in the hydrolyzed guar gum groups was closely related to the varied molecular weight (Mw) of the hydrolysate.
32505987	0	19	theme	Dietary	0:6	arg1	galactosyl					8:17	Dietary galactosyl and mannosyl carbohydrates	0:44	galactosyl	8:17	Dietary galactosyl and mannosyl carbohydrates: In-vitro assessment of prebiotic effects.
32505987	6	20	theme	C.	907:908	arg1	butyricum					910:918	C. butyricum	907:918	C. butyricum	907:918	Combined application of MOS and C. butyricum increased the abundance of Clostridiaceae_1.
32505987	1	21	theme	prebiotic	93:101	arg1	activities					103:112	The prebiotic activities	89:112	The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa)	89:246	The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa) were evaluated by in vitro fermentation.
32505987	4	22	from	production	608:617	arg1	groups					646:651	the hydrolyzed guar gum groups	622:651	the hydrolyzed guar gum groups	622:651	Moreover, SCFA production in the hydrolyzed guar gum groups was closely related to the varied molecular weight (Mw) of the hydrolysate.
32505987	4	23	theme	hydrolyzed	626:635	arg1	gum					642:644	the hydrolyzed guar gum	622:644	the hydrolyzed guar gum groups	622:651	Moreover, SCFA production in the hydrolyzed guar gum groups was closely related to the varied molecular weight (Mw) of the hydrolysate.
32505987	0	24	theme	mannosyl	23:30	arg1	carbohydrates					32:44	Dietary galactosyl and mannosyl carbohydrates	0:44	carbohydrates	32:44	Dietary galactosyl and mannosyl carbohydrates: In-vitro assessment of prebiotic effects.
32505987	3	25	theme	strongest	563:571	arg1	effect					585:590	the strongest butyrogenic effect	559:590	the strongest butyrogenic effect	559:590	GOS and GMOS markedly promoted the growth of bifidobacteria and Clostridium butyricum, respectively, whereas MOS showed the strongest butyrogenic effect.
32505987	3	26	theme	butyricum	515:523	arg1	growth					474:479	the growth	470:479	the growth of bifidobacteria and Clostridium butyricum	470:523	GOS and GMOS markedly promoted the growth of bifidobacteria and Clostridium butyricum, respectively, whereas MOS showed the strongest butyrogenic effect.
32505987	2	27	theme	acid	388:391	arg1	-production					399:409	short-chain fatty acid (SCFA)-production	370:409	short-chain fatty acid (SCFA)-production	370:409	The tested carbohydrates showed selective prebiotic effects on bacterial growth, short-chain fatty acid (SCFA)-production, and substrate consumption.
32505987	3	28	dep	promoted	461:468	arg1	whereas					540:546	whereas	540:546	whereas	540:546	GOS and GMOS markedly promoted the growth of bifidobacteria and Clostridium butyricum, respectively, whereas MOS showed the strongest butyrogenic effect.
32505987	6	29	theme	MOS	899:901	arg1	application					884:894	Combined application	875:894	Combined application of MOS and C. butyricum	875:918	Combined application of MOS and C. butyricum increased the abundance of Clostridiaceae_1.
32505987	1	30	theme	manno-oligosaccharide	169:189	arg1	activities					103:112	The prebiotic activities	89:112	The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa)	89:246	The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa) were evaluated by in vitro fermentation.
32505987	7	31	theme	chemical	1108:1115	arg1	structures					1117:1126	their chemical structures	1102:1126	their chemical structures including their glycoside composition and Mw	1102:1171	Overall, our results indicate that galactosyl and mannosyl carbohydrates have individualized prebiotic effects which are associated with their chemical structures including their glycoside composition and Mw.
32505987	7	31	theme	chemical	1108:1115	arg1	Mw					1170:1171	Mw	1170:1171	Mw	1170:1171	Overall, our results indicate that galactosyl and mannosyl carbohydrates have individualized prebiotic effects which are associated with their chemical structures including their glycoside composition and Mw.
32505987	7	31	theme	chemical	1108:1115	arg1	composition					1154:1164	their glycoside composition	1138:1164	their glycoside composition	1138:1164	Overall, our results indicate that galactosyl and mannosyl carbohydrates have individualized prebiotic effects which are associated with their chemical structures including their glycoside composition and Mw.
32505987	5	32	theme	butyrate	841:848	arg1	yields					806:811	the highest yields	794:811	the highest yields of lactate, propionate, and butyrate	794:848	During in vitro fermentation with human fecal inocula, GMOS gave the highest yields of lactate, propionate, and butyrate after 48 h fermentation.
32505987	2	33	theme	short-chain	370:380	arg1	SCFA					394:397	SCFA	394:397	SCFA	394:397	The tested carbohydrates showed selective prebiotic effects on bacterial growth, short-chain fatty acid (SCFA)-production, and substrate consumption.
32505987	2	33	theme	short-chain	370:380	arg1	acid					388:391	short-chain fatty acid	370:391	short-chain fatty acid (SCFA)-production	370:409	The tested carbohydrates showed selective prebiotic effects on bacterial growth, short-chain fatty acid (SCFA)-production, and substrate consumption.
32505987	2	34	theme	substrate	416:424	arg1	consumption					426:436	substrate consumption	416:436	substrate consumption	416:436	The tested carbohydrates showed selective prebiotic effects on bacterial growth, short-chain fatty acid (SCFA)-production, and substrate consumption.
32505987	5	35	theme	human	763:767	arg1	inocula					775:781	human fecal inocula	763:781	human fecal inocula	763:781	During in vitro fermentation with human fecal inocula, GMOS gave the highest yields of lactate, propionate, and butyrate after 48 h fermentation.
32505987	4	36	theme	SCFA	603:606	arg1	production					608:617	SCFA production	603:617	SCFA production in the hydrolyzed guar gum groups	603:651	Moreover, SCFA production in the hydrolyzed guar gum groups was closely related to the varied molecular weight (Mw) of the hydrolysate.
32505987	5	37	theme	in	736:737	arg1	fermentation					745:756	in vitro fermentation	736:756	in vitro fermentation with human fecal inocula	736:781	During in vitro fermentation with human fecal inocula, GMOS gave the highest yields of lactate, propionate, and butyrate after 48 h fermentation.
32505987	5	38	theme	lactate	816:822	arg1	yields					806:811	the highest yields	794:811	the highest yields of lactate, propionate, and butyrate	794:848	During in vitro fermentation with human fecal inocula, GMOS gave the highest yields of lactate, propionate, and butyrate after 48 h fermentation.
32505987	2	39	from	effects	341:347	arg1	-production					399:409	short-chain fatty acid (SCFA)-production	370:409	short-chain fatty acid (SCFA)-production	370:409	The tested carbohydrates showed selective prebiotic effects on bacterial growth, short-chain fatty acid (SCFA)-production, and substrate consumption.
32505987	2	39	from	effects	341:347	arg1	growth					362:367	bacterial growth	352:367	bacterial growth	352:367	The tested carbohydrates showed selective prebiotic effects on bacterial growth, short-chain fatty acid (SCFA)-production, and substrate consumption.
32505987	2	39	from	effects	341:347	arg1	consumption					426:436	substrate consumption	416:436	substrate consumption	416:436	The tested carbohydrates showed selective prebiotic effects on bacterial growth, short-chain fatty acid (SCFA)-production, and substrate consumption.
32505987	1	40	theme	hydrolyzed	117:126	arg1	gum					133:135	hydrolyzed guar gum	117:135	hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa)	117:166	The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa) were evaluated by in vitro fermentation.
32505987	1	40	theme	hydrolyzed	117:126	arg1	<1 kDa					144:149	<1 kDa	144:149	<1 kDa	144:149	The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa) were evaluated by in vitro fermentation.
32505987	0	41	theme	In-vitro	47:54	arg1	assessment					56:65	In-vitro assessment	47:65	Dietary galactosyl and mannosyl carbohydrates: In-vitro assessment of prebiotic effects.	0:87	Dietary galactosyl and mannosyl carbohydrates: In-vitro assessment of prebiotic effects.
32505987	1	42	theme	in	266:267	arg1	fermentation					275:286	in vitro fermentation	266:286	in vitro fermentation	266:286	The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa) were evaluated by in vitro fermentation.
32505987	1	43	theme	guar	128:131	arg1	gum					133:135	hydrolyzed guar gum	117:135	hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa)	117:166	The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa) were evaluated by in vitro fermentation.
32505987	1	43	theme	guar	128:131	arg1	<1 kDa					144:149	<1 kDa	144:149	<1 kDa	144:149	The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa) were evaluated by in vitro fermentation.
32505987	7	44	contain	have	1038:1041	arg1	galactosyl					1000:1009	galactosyl	1000:1009	galactosyl	1000:1009	Overall, our results indicate that galactosyl and mannosyl carbohydrates have individualized prebiotic effects which are associated with their chemical structures including their glycoside composition and Mw.
32505987	7	44	contain	have	1038:1041	arg2	effects					1068:1074	individualized prebiotic effects	1043:1074	individualized prebiotic effects which are associated with their chemical structures including their glycoside composition and Mw	1043:1171	Overall, our results indicate that galactosyl and mannosyl carbohydrates have individualized prebiotic effects which are associated with their chemical structures including their glycoside composition and Mw.
32505987	7	44	contain	have	1038:1041	arg1	mannosyl					1015:1022	mannosyl	1015:1022	mannosyl	1015:1022	Overall, our results indicate that galactosyl and mannosyl carbohydrates have individualized prebiotic effects which are associated with their chemical structures including their glycoside composition and Mw.
32505987	3	45	theme	butyrogenic	573:583	arg1	effect					585:590	the strongest butyrogenic effect	559:590	the strongest butyrogenic effect	559:590	GOS and GMOS markedly promoted the growth of bifidobacteria and Clostridium butyricum, respectively, whereas MOS showed the strongest butyrogenic effect.
32505987	1	46	theme	gum	133:135	arg1	activities					103:112	The prebiotic activities	89:112	The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa)	89:246	The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa) were evaluated by in vitro fermentation.
32505987	5	47	with	fermentation	745:756	arg1	inocula					775:781	human fecal inocula	763:781	human fecal inocula	763:781	During in vitro fermentation with human fecal inocula, GMOS gave the highest yields of lactate, propionate, and butyrate after 48 h fermentation.
32505987	5	48	theme	fecal	769:773	arg1	inocula					775:781	human fecal inocula	763:781	human fecal inocula	763:781	During in vitro fermentation with human fecal inocula, GMOS gave the highest yields of lactate, propionate, and butyrate after 48 h fermentation.
32505987	4	49	theme	gum	642:644	arg1	groups					646:651	the hydrolyzed guar gum groups	622:651	the hydrolyzed guar gum groups	622:651	Moreover, SCFA production in the hydrolyzed guar gum groups was closely related to the varied molecular weight (Mw) of the hydrolysate.
32505987	4	50	theme	varied	680:685	arg1	Mw					705:706	Mw	705:706	Mw	705:706	Moreover, SCFA production in the hydrolyzed guar gum groups was closely related to the varied molecular weight (Mw) of the hydrolysate.
32505987	4	50	theme	varied	680:685	arg1	weight					697:702	the varied molecular weight	676:702	the varied molecular weight (Mw) of the hydrolysate	676:726	Moreover, SCFA production in the hydrolyzed guar gum groups was closely related to the varied molecular weight (Mw) of the hydrolysate.
32505987	0	51	theme	prebiotic	70:78	arg1	effects					80:86	prebiotic effects	70:86	prebiotic effects	70:86	Dietary galactosyl and mannosyl carbohydrates: In-vitro assessment of prebiotic effects.
32505987	1	52	dep	galacto-oligosaccharide	210:232	arg1	<1 kDa					240:245	<1 kDa	240:245	<1 kDa	240:245	The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa) were evaluated by in vitro fermentation.
32505987	1	52	dep	galacto-oligosaccharide	210:232	arg1	GOS					235:237	GOS	235:237	GOS	235:237	The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa) were evaluated by in vitro fermentation.
32505987	0	53	dep	galactosyl	8:17	arg1	assessment					56:65	In-vitro assessment	47:65	Dietary galactosyl and mannosyl carbohydrates: In-vitro assessment of prebiotic effects.	0:87	Dietary galactosyl and mannosyl carbohydrates: In-vitro assessment of prebiotic effects.
32505987	2	54	theme	prebiotic	331:339	arg1	effects					341:347	selective prebiotic effects	321:347	selective prebiotic effects on bacterial growth, short-chain fatty acid (SCFA)-production, and substrate consumption	321:436	The tested carbohydrates showed selective prebiotic effects on bacterial growth, short-chain fatty acid (SCFA)-production, and substrate consumption.
32505987	5	55	theme	highest	798:804	arg1	yields					806:811	the highest yields	794:811	the highest yields of lactate, propionate, and butyrate	794:848	During in vitro fermentation with human fecal inocula, GMOS gave the highest yields of lactate, propionate, and butyrate after 48 h fermentation.
32505987	6	56	theme	Clostridiaceae_1	947:962	arg1	abundance					934:942	the abundance	930:942	the abundance of Clostridiaceae_1	930:962	Combined application of MOS and C. butyricum increased the abundance of Clostridiaceae_1.
32505987	7	57	dep	galactosyl	1000:1009	arg1	carbohydrates					1024:1036	carbohydrates	1024:1036	carbohydrates	1024:1036	Overall, our results indicate that galactosyl and mannosyl carbohydrates have individualized prebiotic effects which are associated with their chemical structures including their glycoside composition and Mw.
32505987	1	58	dep	manno-oligosaccharide	169:189	arg1	<1 kDa					197:202	<1 kDa	197:202	<1 kDa	197:202	The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa) were evaluated by in vitro fermentation.
32505987	1	58	dep	manno-oligosaccharide	169:189	arg1	MOS					192:194	MOS	192:194	MOS	192:194	The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa) were evaluated by in vitro fermentation.
32505987	2	59	theme	selective	321:329	arg1	effects					341:347	selective prebiotic effects	321:347	selective prebiotic effects on bacterial growth, short-chain fatty acid (SCFA)-production, and substrate consumption	321:436	The tested carbohydrates showed selective prebiotic effects on bacterial growth, short-chain fatty acid (SCFA)-production, and substrate consumption.
32505987	6	60	theme	Combined	875:882	arg1	application					884:894	Combined application	875:894	Combined application of MOS and C. butyricum	875:918	Combined application of MOS and C. butyricum increased the abundance of Clostridiaceae_1.
34886919	0	0	theme	de	73:74	arg1	leche					76:80	dulce de leche	67:80	dulce de leche	67:80	Influence of sucrose reduction on fouling during the production of dulce de leche.
34886919	8	1	theme	better	1355:1360	arg1	manufacturing					1362:1374	better manufacturing	1355:1374	better manufacturing	1355:1374	Therefore, these impacts must be considered in order to maintain better manufacturing and ensure efficient cleaning of equipment.
34886919	1	2	theme	industry´s	132:141	arg1	tendency					149:156	the food industry´s broad tendency	123:156	the food industry´s broad tendency to decrease sugar content in food products onto dulce de leche (DL) and examine the influence of sucrose reduction on the detrimental deposits formed during the production process	123:336	In this Research Communication we focus the food industry´s broad tendency to decrease sugar content in food products onto dulce de leche (DL) and examine the influence of sucrose reduction on the detrimental deposits formed during the production process.
34886919	0	3	theme	dulce	67:71	arg1	leche					76:80	dulce de leche	67:80	dulce de leche	67:80	Influence of sucrose reduction on fouling during the production of dulce de leche.
34886919	3	4	theme	w/w	590:592	arg1	sucrose					560:566	sucrose	560:566	sucrose (20, 15, 10, 5 and 0% w/w)	560:593	Different percentages of sucrose (20, 15, 10, 5 and 0% w/w) were submitted to the DL concentration process in a process simulator.
34886919	3	4	theme	w/w	590:592	arg1	%					588:588	20, 15, 10, 5 and 0% w/w	569:592	20, 15, 10, 5 and 0% w/w	569:592	Different percentages of sucrose (20, 15, 10, 5 and 0% w/w) were submitted to the DL concentration process in a process simulator.
34886919	1	5	theme	broad	143:147	arg1	tendency					149:156	the food industry´s broad tendency	123:156	the food industry´s broad tendency to decrease sugar content in food products onto dulce de leche (DL) and examine the influence of sucrose reduction on the detrimental deposits formed during the production process	123:336	In this Research Communication we focus the food industry´s broad tendency to decrease sugar content in food products onto dulce de leche (DL) and examine the influence of sucrose reduction on the detrimental deposits formed during the production process.
34886919	0	6	from	Influence	0:8	arg1	fouling					34:40	fouling	34:40	fouling during the production of dulce de leche	34:80	Influence of sucrose reduction on fouling during the production of dulce de leche.
34886919	6	7	from	changes	970:976	arg1	formulation					985:995	the formulation	981:995	the formulation that directly impact the formation of deposits in the equipment used (fouling)	981:1074	Thus, the work highlights the need to change the product manufacturing process due to changes in the formulation that directly impact the formation of deposits in the equipment used (fouling).
34886919	0	8	theme	leche	76:80	arg1	production					53:62	the production	49:62	the production of dulce de leche	49:80	Influence of sucrose reduction on fouling during the production of dulce de leche.
34886919	3	9	theme	DL	617:618	arg1	process					634:640	the DL concentration process	613:640	the DL concentration process in a process simulator	613:663	Different percentages of sucrose (20, 15, 10, 5 and 0% w/w) were submitted to the DL concentration process in a process simulator.
34886919	6	10	from	equipment	1051:1059	arg1	formation					1022:1030	the formation	1018:1030	the formation of deposits in the equipment used (fouling)	1018:1074	Thus, the work highlights the need to change the product manufacturing process due to changes in the formulation that directly impact the formation of deposits in the equipment used (fouling).
34886919	2	11	theme	sucrose	465:471	arg1	content					473:479	low sucrose content	461:479	low sucrose content required varying the quantity of sucrose in the milk	461:532	The method used to identify the impact produced directly on the heat exchanger during the production of this product with low sucrose content required varying the quantity of sucrose in the milk.
34886919	1	12	theme	production	319:328	arg1	process					330:336	the production process	315:336	the production process	315:336	In this Research Communication we focus the food industry´s broad tendency to decrease sugar content in food products onto dulce de leche (DL) and examine the influence of sucrose reduction on the detrimental deposits formed during the production process.
34886919	1	13	from	influence	242:250	arg1	deposits					292:299	the detrimental deposits	276:299	the detrimental deposits formed during the production process	276:336	In this Research Communication we focus the food industry´s broad tendency to decrease sugar content in food products onto dulce de leche (DL) and examine the influence of sucrose reduction on the detrimental deposits formed during the production process.
34886919	2	14	with	product	448:454	arg1	content					473:479	low sucrose content	461:479	low sucrose content required varying the quantity of sucrose in the milk	461:532	The method used to identify the impact produced directly on the heat exchanger during the production of this product with low sucrose content required varying the quantity of sucrose in the milk.
34886919	2	15	theme	low	461:463	arg1	content					473:479	low sucrose content	461:479	low sucrose content required varying the quantity of sucrose in the milk	461:532	The method used to identify the impact produced directly on the heat exchanger during the production of this product with low sucrose content required varying the quantity of sucrose in the milk.
34886919	8	16	theme	equipment	1409:1417	arg1	cleaning					1397:1404	efficient cleaning	1387:1404	efficient cleaning of equipment	1387:1417	Therefore, these impacts must be considered in order to maintain better manufacturing and ensure efficient cleaning of equipment.
34886919	7	17	from	reduction	1226:1234	arg1	production					1266:1275	the production	1262:1275	the production	1262:1275	This deposit changes significantly in relation to its quantity as well as in relation to the composition and chemical characteristics as the gradual reduction of the sucrose content in the production takes place.
34886919	6	18	theme	due	963:965	arg1	process					955:961	the product manufacturing process	929:961	the product manufacturing process due to changes in the formulation that directly impact the formation of deposits in the equipment used (fouling)	929:1074	Thus, the work highlights the need to change the product manufacturing process due to changes in the formulation that directly impact the formation of deposits in the equipment used (fouling).
34886919	2	19	theme	heat	403:406	arg1	exchanger					408:416	the heat exchanger	399:416	the heat exchanger	399:416	The method used to identify the impact produced directly on the heat exchanger during the production of this product with low sucrose content required varying the quantity of sucrose in the milk.
34886919	3	20	theme	concentration	620:632	arg1	process					634:640	the DL concentration process	613:640	the DL concentration process in a process simulator	613:663	Different percentages of sucrose (20, 15, 10, 5 and 0% w/w) were submitted to the DL concentration process in a process simulator.
34886919	3	21	from	process	634:640	arg1	simulator					655:663	a process simulator	645:663	a process simulator	645:663	Different percentages of sucrose (20, 15, 10, 5 and 0% w/w) were submitted to the DL concentration process in a process simulator.
34886919	1	22	theme	sugar	170:174	arg1	content					176:182	sugar content	170:182	sugar content in food products onto dulce de leche (DL)	170:224	In this Research Communication we focus the food industry´s broad tendency to decrease sugar content in food products onto dulce de leche (DL) and examine the influence of sucrose reduction on the detrimental deposits formed during the production process.
34886919	0	23	theme	reduction	21:29	arg1	Influence					0:8	Influence	0:8	Influence of sucrose reduction on fouling during the production of dulce de leche.	0:81	Influence of sucrose reduction on fouling during the production of dulce de leche.
34886919	3	24	theme	process	647:653	arg1	simulator					655:663	a process simulator	645:663	a process simulator	645:663	Different percentages of sucrose (20, 15, 10, 5 and 0% w/w) were submitted to the DL concentration process in a process simulator.
34886919	7	25	theme	sucrose	1243:1249	arg1	content					1251:1257	the sucrose content	1239:1257	the sucrose content	1239:1257	This deposit changes significantly in relation to its quantity as well as in relation to the composition and chemical characteristics as the gradual reduction of the sucrose content in the production takes place.
34886919	6	26	from	formation	1022:1030	arg1	equipment					1051:1059	the equipment	1047:1059	the equipment used (fouling)	1047:1074	Thus, the work highlights the need to change the product manufacturing process due to changes in the formulation that directly impact the formation of deposits in the equipment used (fouling).
34886919	5	27	used	used	878:881	arg2	sem-EDS					865:871	sem-EDS	865:871	sem-EDS	865:871	Methods such as Kjeldahl, Pregl-Dumas and sem-EDS were used.
34886919	5	27	used	used	878:881	arg2	Methods					823:829	Methods	823:829	Methods such as Kjeldahl, Pregl-Dumas and sem-EDS	823:871	Methods such as Kjeldahl, Pregl-Dumas and sem-EDS were used.
34886919	5	27	used	used	878:881	arg2	Pregl-Dumas					849:859	Pregl-Dumas	849:859	Pregl-Dumas	849:859	Methods such as Kjeldahl, Pregl-Dumas and sem-EDS were used.
34886919	5	27	used	used	878:881	arg2	Kjeldahl					839:846	Kjeldahl	839:846	Kjeldahl	839:846	Methods such as Kjeldahl, Pregl-Dumas and sem-EDS were used.
34886919	0	28	theme	sucrose	13:19	arg1	reduction					21:29	sucrose reduction	13:29	sucrose reduction	13:29	Influence of sucrose reduction on fouling during the production of dulce de leche.
34886919	8	29	theme	efficient	1387:1395	arg1	cleaning					1397:1404	efficient cleaning	1387:1404	efficient cleaning of equipment	1387:1417	Therefore, these impacts must be considered in order to maintain better manufacturing and ensure efficient cleaning of equipment.
34886919	6	30	from	deposits	1035:1042	arg1	equipment					1051:1059	the equipment	1047:1059	the equipment used (fouling)	1047:1074	Thus, the work highlights the need to change the product manufacturing process due to changes in the formulation that directly impact the formation of deposits in the equipment used (fouling).
34886919	6	31	theme	deposits	1035:1042	arg1	formation					1022:1030	the formation	1018:1030	the formation of deposits in the equipment used (fouling)	1018:1074	Thus, the work highlights the need to change the product manufacturing process due to changes in the formulation that directly impact the formation of deposits in the equipment used (fouling).
34886919	4	32	theme	deposits	713:720	arg1	quantification					691:704	the quantification	687:704	the quantification of the deposits formed in each	687:735	After concentration, the quantification of the deposits formed in each was carried out and these deposits were characterized according to their composition.
34886919	6	33	theme	manufacturing	941:953	arg1	process					955:961	the product manufacturing process	929:961	the product manufacturing process due to changes in the formulation that directly impact the formation of deposits in the equipment used (fouling)	929:1074	Thus, the work highlights the need to change the product manufacturing process due to changes in the formulation that directly impact the formation of deposits in the equipment used (fouling).
34886919	1	34	theme	food	187:190	arg1	products					192:199	food products	187:199	food products onto dulce de leche (DL)	187:224	In this Research Communication we focus the food industry´s broad tendency to decrease sugar content in food products onto dulce de leche (DL) and examine the influence of sucrose reduction on the detrimental deposits formed during the production process.
34886919	1	35	theme	sucrose	255:261	arg1	reduction					263:271	sucrose reduction	255:271	sucrose reduction	255:271	In this Research Communication we focus the food industry´s broad tendency to decrease sugar content in food products onto dulce de leche (DL) and examine the influence of sucrose reduction on the detrimental deposits formed during the production process.
34886919	1	36	theme	reduction	263:271	arg1	influence					242:250	the influence	238:250	the influence of sucrose reduction on the detrimental deposits formed during the production process	238:336	In this Research Communication we focus the food industry´s broad tendency to decrease sugar content in food products onto dulce de leche (DL) and examine the influence of sucrose reduction on the detrimental deposits formed during the production process.
34886919	1	37	theme	Research	91:98	arg1	Communication					100:112	this Research Communication	86:112	this Research Communication	86:112	In this Research Communication we focus the food industry´s broad tendency to decrease sugar content in food products onto dulce de leche (DL) and examine the influence of sucrose reduction on the detrimental deposits formed during the production process.
34886919	7	38	theme	chemical	1186:1193	arg1	characteristics					1195:1209	chemical characteristics	1186:1209	chemical characteristics	1186:1209	This deposit changes significantly in relation to its quantity as well as in relation to the composition and chemical characteristics as the gradual reduction of the sucrose content in the production takes place.
34886919	3	39	theme	Different	535:543	arg1	percentages					545:555	Different percentages	535:555	Different percentages of sucrose (20, 15, 10, 5 and 0% w/w)	535:593	Different percentages of sucrose (20, 15, 10, 5 and 0% w/w) were submitted to the DL concentration process in a process simulator.
34886919	1	40	from	content	176:182	arg1	products					192:199	food products	187:199	food products onto dulce de leche (DL)	187:224	In this Research Communication we focus the food industry´s broad tendency to decrease sugar content in food products onto dulce de leche (DL) and examine the influence of sucrose reduction on the detrimental deposits formed during the production process.
34886919	7	41	dep	composition	1170:1180	arg1	the					1166:1168	the	1166:1168	the	1166:1168	This deposit changes significantly in relation to its quantity as well as in relation to the composition and chemical characteristics as the gradual reduction of the sucrose content in the production takes place.
34886919	7	41	dep	composition	1170:1180	arg1	takes					1277:1281	takes	1277:1281	takes place	1277:1287	This deposit changes significantly in relation to its quantity as well as in relation to the composition and chemical characteristics as the gradual reduction of the sucrose content in the production takes place.
34886919	2	42	theme	sucrose	514:520	arg1	quantity					502:509	the quantity	498:509	the quantity of sucrose in the milk	498:532	The method used to identify the impact produced directly on the heat exchanger during the production of this product with low sucrose content required varying the quantity of sucrose in the milk.
34886919	7	43	theme	content	1251:1257	arg1	reduction					1226:1234	the gradual reduction	1214:1234	the gradual reduction of the sucrose content in the production	1214:1275	This deposit changes significantly in relation to its quantity as well as in relation to the composition and chemical characteristics as the gradual reduction of the sucrose content in the production takes place.
34886919	1	44	theme	dulce	206:210	arg1	DL					222:223	DL	222:223	DL	222:223	In this Research Communication we focus the food industry´s broad tendency to decrease sugar content in food products onto dulce de leche (DL) and examine the influence of sucrose reduction on the detrimental deposits formed during the production process.
34886919	1	44	theme	dulce	206:210	arg1	leche					215:219	dulce de leche	206:219	dulce de leche (DL)	206:224	In this Research Communication we focus the food industry´s broad tendency to decrease sugar content in food products onto dulce de leche (DL) and examine the influence of sucrose reduction on the detrimental deposits formed during the production process.
34886919	3	45	theme	sucrose	560:566	arg1	percentages					545:555	Different percentages	535:555	Different percentages of sucrose (20, 15, 10, 5 and 0% w/w)	535:593	Different percentages of sucrose (20, 15, 10, 5 and 0% w/w) were submitted to the DL concentration process in a process simulator.
34886919	6	46	theme	product	933:939	arg1	process					955:961	the product manufacturing process	929:961	the product manufacturing process due to changes in the formulation that directly impact the formation of deposits in the equipment used (fouling)	929:1074	Thus, the work highlights the need to change the product manufacturing process due to changes in the formulation that directly impact the formation of deposits in the equipment used (fouling).
34886919	1	47	theme	de	212:213	arg1	DL					222:223	DL	222:223	DL	222:223	In this Research Communication we focus the food industry´s broad tendency to decrease sugar content in food products onto dulce de leche (DL) and examine the influence of sucrose reduction on the detrimental deposits formed during the production process.
34886919	1	47	theme	de	212:213	arg1	leche					215:219	dulce de leche	206:219	dulce de leche (DL)	206:224	In this Research Communication we focus the food industry´s broad tendency to decrease sugar content in food products onto dulce de leche (DL) and examine the influence of sucrose reduction on the detrimental deposits formed during the production process.
34886919	2	48	theme	product	448:454	arg1	production					429:438	the production	425:438	the production of this product with low sucrose content required varying the quantity of sucrose in the milk	425:532	The method used to identify the impact produced directly on the heat exchanger during the production of this product with low sucrose content required varying the quantity of sucrose in the milk.
34886919	1	49	theme	detrimental	280:290	arg1	deposits					292:299	the detrimental deposits	276:299	the detrimental deposits formed during the production process	276:336	In this Research Communication we focus the food industry´s broad tendency to decrease sugar content in food products onto dulce de leche (DL) and examine the influence of sucrose reduction on the detrimental deposits formed during the production process.
34886919	7	50	theme	gradual	1218:1224	arg1	reduction					1226:1234	the gradual reduction	1214:1234	the gradual reduction of the sucrose content in the production	1214:1275	This deposit changes significantly in relation to its quantity as well as in relation to the composition and chemical characteristics as the gradual reduction of the sucrose content in the production takes place.
34886919	1	51	theme	food	127:130	arg1	tendency					149:156	the food industry´s broad tendency	123:156	the food industry´s broad tendency to decrease sugar content in food products onto dulce de leche (DL) and examine the influence of sucrose reduction on the detrimental deposits formed during the production process	123:336	In this Research Communication we focus the food industry´s broad tendency to decrease sugar content in food products onto dulce de leche (DL) and examine the influence of sucrose reduction on the detrimental deposits formed during the production process.
34886919	2	52	from	quantity	502:509	arg1	milk					529:532	the milk	525:532	the milk	525:532	The method used to identify the impact produced directly on the heat exchanger during the production of this product with low sucrose content required varying the quantity of sucrose in the milk.
32320808	2	0	theme	I	564:564	arg1	collagen					566:573	type I collagen	559:573	type I collagen	559:573	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	2	0	theme	I	564:564	arg1	components-chitosan					538:556	the natural extracellular matrix (ECM) components-chitosan	499:556	the natural extracellular matrix (ECM) components-chitosan	499:556	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	4	1	theme	calcified	771:779	arg1	cartilage					781:789	The calcified cartilage and cartilage layers	767:810	cartilage	781:789	The calcified cartilage and cartilage layers were prepared by thermal gelation method in the hydrogel form.
32320808	1	2	theme	mimetic	212:218	arg1	constructs					220:229	multi-layered/multi-component osteochondral mimetic constructs	168:229	multi-layered/multi-component osteochondral mimetic constructs	168:229	Tissue engineering approach offers alternative strategies to develop multi-layered/multi-component osteochondral mimetic constructs to meet the requirements of the heterogeneous and layered structure of native osteochondral tissue.
32320808	4	3	theme	hydrogel	860:867	arg1	form					869:872	the hydrogel form	856:872	the hydrogel form	856:872	The calcified cartilage and cartilage layers were prepared by thermal gelation method in the hydrogel form.
32320808	1	4	theme	osteochondral	309:321	arg1	tissue					323:328	native osteochondral tissue	302:328	native osteochondral tissue	302:328	Tissue engineering approach offers alternative strategies to develop multi-layered/multi-component osteochondral mimetic constructs to meet the requirements of the heterogeneous and layered structure of native osteochondral tissue.
32320808	2	5	theme	type	559:562	arg1	collagen					566:573	type I collagen	559:573	type I collagen	559:573	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	2	5	theme	type	559:562	arg1	components-chitosan					538:556	the natural extracellular matrix (ECM) components-chitosan	499:556	the natural extracellular matrix (ECM) components-chitosan	499:556	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	7	6	theme	calcified	1289:1297	arg1	cartilage					1299:1307	calcified cartilage	1289:1307	calcified cartilage	1289:1307	Expression of genes specific for bone, calcified cartilage and cartilage were improved in the osteochondral scaffold.
32320808	2	7	theme	gradient	416:423	arg1	composition					425:435	a gradient composition	414:435	a gradient composition	414:435	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	1	8	theme	tissue	323:328	arg1	structure					289:297	the heterogeneous and layered structure	259:297	the heterogeneous and layered structure of native osteochondral tissue	259:328	Tissue engineering approach offers alternative strategies to develop multi-layered/multi-component osteochondral mimetic constructs to meet the requirements of the heterogeneous and layered structure of native osteochondral tissue.
32320808	6	9	theme	matrix	1172:1177	arg1	production					1179:1188	enhanced extracellular matrix production	1149:1188	enhanced extracellular matrix production	1149:1188	Histology and biochemical analysis showed enhanced extracellular matrix production and demonstrated collagen and glycosaminoglycan deposition.
32320808	2	10	theme	overlaying	352:361	arg1	process					363:369	an iterative overlaying process	339:369	an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure	339:464	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	5	11	theme	preosteoblasts	1068:1081	arg1	co-culture					1045:1054	a co-culture	1043:1054	a co-culture of MC3T3-E1 preosteoblasts and ATDC5 chondrocytes	1043:1104	Osteochondral scaffolds fabricated by iterative overlaying of each distinct layer exhibited a porous, continuous gradient structure and supported cell proliferation in a co-culture of MC3T3-E1 preosteoblasts and ATDC5 chondrocytes.
32320808	4	12	theme	thermal	829:835	arg1	method					846:851	thermal gelation method	829:851	thermal gelation method	829:851	The calcified cartilage and cartilage layers were prepared by thermal gelation method in the hydrogel form.
32320808	5	13	theme	ATDC5	1087:1091	arg1	chondrocytes					1093:1104	ATDC5 chondrocytes	1087:1104	ATDC5 chondrocytes	1087:1104	Osteochondral scaffolds fabricated by iterative overlaying of each distinct layer exhibited a porous, continuous gradient structure and supported cell proliferation in a co-culture of MC3T3-E1 preosteoblasts and ATDC5 chondrocytes.
32320808	2	14	theme	iterative	342:350	arg1	process					363:369	an iterative overlaying process	339:369	an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure	339:464	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	5	15	theme	cell	1021:1024	arg1	proliferation					1026:1038	cell proliferation	1021:1038	cell proliferation	1021:1038	Osteochondral scaffolds fabricated by iterative overlaying of each distinct layer exhibited a porous, continuous gradient structure and supported cell proliferation in a co-culture of MC3T3-E1 preosteoblasts and ATDC5 chondrocytes.
32320808	7	16	theme	genes	1264:1268	arg1	Expression					1250:1259	Expression	1250:1259	Expression of genes specific for bone, calcified cartilage and cartilage	1250:1321	Expression of genes specific for bone, calcified cartilage and cartilage were improved in the osteochondral scaffold.
32320808	5	17	theme	distinct	942:949	arg1	layer					951:955	each distinct layer	937:955	each distinct layer	937:955	Osteochondral scaffolds fabricated by iterative overlaying of each distinct layer exhibited a porous, continuous gradient structure and supported cell proliferation in a co-culture of MC3T3-E1 preosteoblasts and ATDC5 chondrocytes.
32320808	5	18	theme	chondrocytes	1093:1104	arg1	co-culture					1045:1054	a co-culture	1043:1054	a co-culture of MC3T3-E1 preosteoblasts and ATDC5 chondrocytes	1043:1104	Osteochondral scaffolds fabricated by iterative overlaying of each distinct layer exhibited a porous, continuous gradient structure and supported cell proliferation in a co-culture of MC3T3-E1 preosteoblasts and ATDC5 chondrocytes.
32320808	8	19	theme	freeze-dried	1429:1440	arg1	structures					1565:1574	multi-layered structures	1551:1574	multi-layered structures	1551:1574	Overall, these findings suggest that iterative overlaying of freeze-dried scaffolds and hydrogel matrices prepared by using ECM components in biomimetic ratios to fabricate gradient, multi-layered structures can be a promising strategy without the need for growth factors.
32320808	8	19	theme	freeze-dried	1429:1440	arg1	scaffolds					1442:1450	freeze-dried scaffolds	1429:1450	freeze-dried scaffolds	1429:1450	Overall, these findings suggest that iterative overlaying of freeze-dried scaffolds and hydrogel matrices prepared by using ECM components in biomimetic ratios to fabricate gradient, multi-layered structures can be a promising strategy without the need for growth factors.
32320808	2	20	theme	type	576:579	arg1	components-chitosan					538:556	the natural extracellular matrix (ECM) components-chitosan	499:556	the natural extracellular matrix (ECM) components-chitosan	499:556	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	2	20	theme	type	576:579	arg1	collagen					584:591	type II collagen	576:591	type II collagen	576:591	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	7	21	theme	specific	1270:1277	arg1	genes					1264:1268	genes	1264:1268	genes specific for bone, calcified cartilage and cartilage	1264:1321	Expression of genes specific for bone, calcified cartilage and cartilage were improved in the osteochondral scaffold.
32320808	0	22	theme	Chitosan/collagen	0:16	arg1	constructs					56:65	Chitosan/collagen based biomimetic osteochondral tissue constructs	0:65	Chitosan/collagen based biomimetic osteochondral tissue constructs	0:65	Chitosan/collagen based biomimetic osteochondral tissue constructs: A growth factor-free approach.
32320808	5	23	theme	porous	969:974	arg1	structure					997:1005	a porous, continuous gradient structure	967:1005	a porous, continuous gradient structure	967:1005	Osteochondral scaffolds fabricated by iterative overlaying of each distinct layer exhibited a porous, continuous gradient structure and supported cell proliferation in a co-culture of MC3T3-E1 preosteoblasts and ATDC5 chondrocytes.
32320808	0	24	theme	based	18:22	arg1	constructs					56:65	Chitosan/collagen based biomimetic osteochondral tissue constructs	0:65	Chitosan/collagen based biomimetic osteochondral tissue constructs	0:65	Chitosan/collagen based biomimetic osteochondral tissue constructs: A growth factor-free approach.
32320808	2	25	from	components-chitosan	538:556	arg1	compositions					656:667	biomimetic compositions	645:667	biomimetic compositions	645:667	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	1	26	theme	Tissue	99:104	arg1	approach					118:125	Tissue engineering approach	99:125	Tissue engineering approach	99:125	Tissue engineering approach offers alternative strategies to develop multi-layered/multi-component osteochondral mimetic constructs to meet the requirements of the heterogeneous and layered structure of native osteochondral tissue.
32320808	6	27	theme	glycosaminoglycan	1220:1236	arg1	deposition					1238:1247	collagen and glycosaminoglycan deposition	1207:1247	deposition	1238:1247	Histology and biochemical analysis showed enhanced extracellular matrix production and demonstrated collagen and glycosaminoglycan deposition.
32320808	2	28	theme	matrix	525:530	arg1	collagen					566:573	type I collagen	559:573	type I collagen	559:573	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	2	28	theme	matrix	525:530	arg1	collagen					584:591	type II collagen	576:591	type II collagen	576:591	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	2	28	theme	matrix	525:530	arg1	nanohydroxyapatite-					594:612	nanohydroxyapatite-	594:612	nanohydroxyapatite-	594:612	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	2	28	theme	matrix	525:530	arg1	components-chitosan					538:556	the natural extracellular matrix (ECM) components-chitosan	499:556	the natural extracellular matrix (ECM) components-chitosan	499:556	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	1	29	theme	engineering	106:116	arg1	approach					118:125	Tissue engineering approach	99:125	Tissue engineering approach	99:125	Tissue engineering approach offers alternative strategies to develop multi-layered/multi-component osteochondral mimetic constructs to meet the requirements of the heterogeneous and layered structure of native osteochondral tissue.
32320808	8	30	theme	scaffolds	1442:1450	arg1	strategy					1595:1602	a promising strategy	1583:1602	a promising strategy	1583:1602	Overall, these findings suggest that iterative overlaying of freeze-dried scaffolds and hydrogel matrices prepared by using ECM components in biomimetic ratios to fabricate gradient, multi-layered structures can be a promising strategy without the need for growth factors.
32320808	8	30	theme	scaffolds	1442:1450	arg1	iterative					1405:1413	iterative	1405:1413	iterative	1405:1413	Overall, these findings suggest that iterative overlaying of freeze-dried scaffolds and hydrogel matrices prepared by using ECM components in biomimetic ratios to fabricate gradient, multi-layered structures can be a promising strategy without the need for growth factors.
32320808	2	31	theme	specific	447:454	arg1	structure					456:464	layer specific structure	441:464	layer specific structure	441:464	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	0	32	theme	osteochondral	35:47	arg1	constructs					56:65	Chitosan/collagen based biomimetic osteochondral tissue constructs	0:65	Chitosan/collagen based biomimetic osteochondral tissue constructs	0:65	Chitosan/collagen based biomimetic osteochondral tissue constructs: A growth factor-free approach.
32320808	2	33	theme	osteochondral	621:633	arg1	tissue					635:640	the osteochondral tissue	617:640	the osteochondral tissue	617:640	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	5	34	dep	porous	969:974	arg1	continuous					977:986	continuous	977:986	continuous	977:986	Osteochondral scaffolds fabricated by iterative overlaying of each distinct layer exhibited a porous, continuous gradient structure and supported cell proliferation in a co-culture of MC3T3-E1 preosteoblasts and ATDC5 chondrocytes.
32320808	5	35	theme	MC3T3-E1	1059:1066	arg1	preosteoblasts					1068:1081	MC3T3-E1 preosteoblasts	1059:1081	MC3T3-E1 preosteoblasts	1059:1081	Osteochondral scaffolds fabricated by iterative overlaying of each distinct layer exhibited a porous, continuous gradient structure and supported cell proliferation in a co-culture of MC3T3-E1 preosteoblasts and ATDC5 chondrocytes.
32320808	8	36	theme	promising	1585:1593	arg1	strategy					1595:1602	a promising strategy	1583:1602	a promising strategy	1583:1602	Overall, these findings suggest that iterative overlaying of freeze-dried scaffolds and hydrogel matrices prepared by using ECM components in biomimetic ratios to fabricate gradient, multi-layered structures can be a promising strategy without the need for growth factors.
32320808	8	36	theme	promising	1585:1593	arg1	iterative					1405:1413	iterative	1405:1413	iterative	1405:1413	Overall, these findings suggest that iterative overlaying of freeze-dried scaffolds and hydrogel matrices prepared by using ECM components in biomimetic ratios to fabricate gradient, multi-layered structures can be a promising strategy without the need for growth factors.
32320808	6	37	theme	collagen	1207:1214	arg1	deposition					1238:1247	collagen and glycosaminoglycan deposition	1207:1247	deposition	1238:1247	Histology and biochemical analysis showed enhanced extracellular matrix production and demonstrated collagen and glycosaminoglycan deposition.
32320808	0	38	dep	approach	89:96	arg1	constructs					56:65	Chitosan/collagen based biomimetic osteochondral tissue constructs	0:65	Chitosan/collagen based biomimetic osteochondral tissue constructs	0:65	Chitosan/collagen based biomimetic osteochondral tissue constructs: A growth factor-free approach.
32320808	4	39	theme	gelation	837:844	arg1	method					846:851	thermal gelation method	829:851	thermal gelation method	829:851	The calcified cartilage and cartilage layers were prepared by thermal gelation method in the hydrogel form.
32320808	2	40	theme	layer	441:445	arg1	structure					456:464	layer specific structure	441:464	layer specific structure	441:464	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	4	41	theme	cartilage	795:803	arg1	layers					805:810	The calcified cartilage and cartilage layers	767:810	layers	805:810	The calcified cartilage and cartilage layers were prepared by thermal gelation method in the hydrogel form.
32320808	5	42	theme	layer	951:955	arg1	iterative					913:921	iterative	913:921	iterative	913:921	Osteochondral scaffolds fabricated by iterative overlaying of each distinct layer exhibited a porous, continuous gradient structure and supported cell proliferation in a co-culture of MC3T3-E1 preosteoblasts and ATDC5 chondrocytes.
32320808	3	43	theme	bone	682:685	arg1	layer					687:691	Subchondral bone layer	670:691	Subchondral bone layer	670:691	Subchondral bone layer was prepared by using freeze-drying method to obtain 3D porous scaffolds.
32320808	1	44	theme	heterogeneous	263:275	arg1	structure					289:297	the heterogeneous and layered structure	259:297	the heterogeneous and layered structure of native osteochondral tissue	259:328	Tissue engineering approach offers alternative strategies to develop multi-layered/multi-component osteochondral mimetic constructs to meet the requirements of the heterogeneous and layered structure of native osteochondral tissue.
32320808	0	45	theme	tissue	49:54	arg1	constructs					56:65	Chitosan/collagen based biomimetic osteochondral tissue constructs	0:65	Chitosan/collagen based biomimetic osteochondral tissue constructs	0:65	Chitosan/collagen based biomimetic osteochondral tissue constructs: A growth factor-free approach.
32320808	5	46	theme	Osteochondral	875:887	arg1	scaffolds					889:897	Osteochondral scaffolds	875:897	Osteochondral scaffolds fabricated by iterative overlaying of each distinct layer	875:955	Osteochondral scaffolds fabricated by iterative overlaying of each distinct layer exhibited a porous, continuous gradient structure and supported cell proliferation in a co-culture of MC3T3-E1 preosteoblasts and ATDC5 chondrocytes.
32320808	3	47	theme	freeze-drying	715:727	arg1	method					729:734	freeze-drying method	715:734	freeze-drying method	715:734	Subchondral bone layer was prepared by using freeze-drying method to obtain 3D porous scaffolds.
32320808	2	48	theme	extracellular	511:523	arg1	matrix					525:530	the natural extracellular matrix	499:530	the natural extracellular matrix (ECM) components-chitosan	499:556	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	2	48	theme	extracellular	511:523	arg1	ECM					533:535	ECM	533:535	ECM	533:535	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	8	49	theme	hydrogel	1456:1463	arg1	matrices					1465:1472	hydrogel matrices	1456:1472	hydrogel matrices	1456:1472	Overall, these findings suggest that iterative overlaying of freeze-dried scaffolds and hydrogel matrices prepared by using ECM components in biomimetic ratios to fabricate gradient, multi-layered structures can be a promising strategy without the need for growth factors.
32320808	5	50	theme	gradient	988:995	arg1	structure					997:1005	a porous, continuous gradient structure	967:1005	a porous, continuous gradient structure	967:1005	Osteochondral scaffolds fabricated by iterative overlaying of each distinct layer exhibited a porous, continuous gradient structure and supported cell proliferation in a co-culture of MC3T3-E1 preosteoblasts and ATDC5 chondrocytes.
32320808	8	51	theme	multi-layered	1551:1563	arg1	structures					1565:1574	multi-layered structures	1551:1574	multi-layered structures	1551:1574	Overall, these findings suggest that iterative overlaying of freeze-dried scaffolds and hydrogel matrices prepared by using ECM components in biomimetic ratios to fabricate gradient, multi-layered structures can be a promising strategy without the need for growth factors.
32320808	8	51	theme	multi-layered	1551:1563	arg1	scaffolds					1442:1450	freeze-dried scaffolds	1429:1450	freeze-dried scaffolds	1429:1450	Overall, these findings suggest that iterative overlaying of freeze-dried scaffolds and hydrogel matrices prepared by using ECM components in biomimetic ratios to fabricate gradient, multi-layered structures can be a promising strategy without the need for growth factors.
32320808	1	52	theme	alternative	134:144	arg1	strategies					146:155	alternative strategies	134:155	alternative strategies	134:155	Tissue engineering approach offers alternative strategies to develop multi-layered/multi-component osteochondral mimetic constructs to meet the requirements of the heterogeneous and layered structure of native osteochondral tissue.
32320808	2	53	theme	biomimetic	645:654	arg1	compositions					656:667	biomimetic compositions	645:667	biomimetic compositions	645:667	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	8	54	theme	biomimetic	1510:1519	arg1	ratios					1521:1526	biomimetic ratios	1510:1526	biomimetic ratios	1510:1526	Overall, these findings suggest that iterative overlaying of freeze-dried scaffolds and hydrogel matrices prepared by using ECM components in biomimetic ratios to fabricate gradient, multi-layered structures can be a promising strategy without the need for growth factors.
32320808	3	55	theme	Subchondral	670:680	arg1	layer					687:691	Subchondral bone layer	670:691	Subchondral bone layer	670:691	Subchondral bone layer was prepared by using freeze-drying method to obtain 3D porous scaffolds.
32320808	2	56	dep	process	363:369	arg1	fabricate					374:382	fabricate	374:382	to fabricate a multi-layered scaffold with a gradient composition and layer specific structure	371:464	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	8	57	from	components	1496:1505	arg1	ratios					1521:1526	biomimetic ratios	1510:1526	biomimetic ratios	1510:1526	Overall, these findings suggest that iterative overlaying of freeze-dried scaffolds and hydrogel matrices prepared by using ECM components in biomimetic ratios to fabricate gradient, multi-layered structures can be a promising strategy without the need for growth factors.
32320808	8	58	theme	matrices	1465:1472	arg1	strategy					1595:1602	a promising strategy	1583:1602	a promising strategy	1583:1602	Overall, these findings suggest that iterative overlaying of freeze-dried scaffolds and hydrogel matrices prepared by using ECM components in biomimetic ratios to fabricate gradient, multi-layered structures can be a promising strategy without the need for growth factors.
32320808	8	58	theme	matrices	1465:1472	arg1	iterative					1405:1413	iterative	1405:1413	iterative	1405:1413	Overall, these findings suggest that iterative overlaying of freeze-dried scaffolds and hydrogel matrices prepared by using ECM components in biomimetic ratios to fabricate gradient, multi-layered structures can be a promising strategy without the need for growth factors.
32320808	6	59	theme	extracellular	1158:1170	arg1	production					1179:1188	enhanced extracellular matrix production	1149:1188	enhanced extracellular matrix production	1149:1188	Histology and biochemical analysis showed enhanced extracellular matrix production and demonstrated collagen and glycosaminoglycan deposition.
32320808	8	60	theme	ECM	1492:1494	arg1	components					1496:1505	ECM components	1492:1505	ECM components in biomimetic ratios	1492:1526	Overall, these findings suggest that iterative overlaying of freeze-dried scaffolds and hydrogel matrices prepared by using ECM components in biomimetic ratios to fabricate gradient, multi-layered structures can be a promising strategy without the need for growth factors.
32320808	1	61	theme	layered	281:287	arg1	structure					289:297	the heterogeneous and layered structure	259:297	the heterogeneous and layered structure of native osteochondral tissue	259:328	Tissue engineering approach offers alternative strategies to develop multi-layered/multi-component osteochondral mimetic constructs to meet the requirements of the heterogeneous and layered structure of native osteochondral tissue.
32320808	2	62	theme	tissue	635:640	arg1	collagen					566:573	type I collagen	559:573	type I collagen	559:573	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	2	62	theme	tissue	635:640	arg1	collagen					584:591	type II collagen	576:591	type II collagen	576:591	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	2	62	theme	tissue	635:640	arg1	nanohydroxyapatite-					594:612	nanohydroxyapatite-	594:612	nanohydroxyapatite-	594:612	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	2	62	theme	tissue	635:640	arg1	components-chitosan					538:556	the natural extracellular matrix (ECM) components-chitosan	499:556	the natural extracellular matrix (ECM) components-chitosan	499:556	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	3	63	theme	3D	746:747	arg1	scaffolds					756:764	3D porous scaffolds	746:764	3D porous scaffolds	746:764	Subchondral bone layer was prepared by using freeze-drying method to obtain 3D porous scaffolds.
32320808	2	64	theme	multi-layered	386:398	arg1	scaffold					400:407	a multi-layered scaffold	384:407	a multi-layered scaffold	384:407	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	1	65	theme	structure	289:297	arg1	requirements					243:254	the requirements	239:254	the requirements of the heterogeneous and layered structure of native osteochondral tissue	239:328	Tissue engineering approach offers alternative strategies to develop multi-layered/multi-component osteochondral mimetic constructs to meet the requirements of the heterogeneous and layered structure of native osteochondral tissue.
32320808	3	66	theme	porous	749:754	arg1	scaffolds					756:764	3D porous scaffolds	746:764	3D porous scaffolds	746:764	Subchondral bone layer was prepared by using freeze-drying method to obtain 3D porous scaffolds.
32320808	8	67	theme	growth	1625:1630	arg1	factors					1632:1638	growth factors	1625:1638	growth factors	1625:1638	Overall, these findings suggest that iterative overlaying of freeze-dried scaffolds and hydrogel matrices prepared by using ECM components in biomimetic ratios to fabricate gradient, multi-layered structures can be a promising strategy without the need for growth factors.
32320808	2	68	theme	natural	503:509	arg1	matrix					525:530	the natural extracellular matrix	499:530	the natural extracellular matrix (ECM) components-chitosan	499:556	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	2	68	theme	natural	503:509	arg1	ECM					533:535	ECM	533:535	ECM	533:535	Herein, an iterative overlaying process to fabricate a multi-layered scaffold with a gradient composition and layer specific structure have been developed by combining the natural extracellular matrix (ECM) components-chitosan, type I collagen, type II collagen, nanohydroxyapatite- of the osteochondral tissue in biomimetic compositions.
32320808	1	69	theme	multi-layered/multi-component	168:196	arg1	constructs					220:229	multi-layered/multi-component osteochondral mimetic constructs	168:229	multi-layered/multi-component osteochondral mimetic constructs	168:229	Tissue engineering approach offers alternative strategies to develop multi-layered/multi-component osteochondral mimetic constructs to meet the requirements of the heterogeneous and layered structure of native osteochondral tissue.
32320808	6	70	theme	biochemical	1121:1131	arg1	analysis					1133:1140	biochemical analysis	1121:1140	biochemical analysis	1121:1140	Histology and biochemical analysis showed enhanced extracellular matrix production and demonstrated collagen and glycosaminoglycan deposition.
32320808	6	71	theme	enhanced	1149:1156	arg1	production					1179:1188	enhanced extracellular matrix production	1149:1188	enhanced extracellular matrix production	1149:1188	Histology and biochemical analysis showed enhanced extracellular matrix production and demonstrated collagen and glycosaminoglycan deposition.
32320808	0	72	theme	growth	70:75	arg1	approach					89:96	A growth factor-free approach	68:96	A growth factor-free approach	68:96	Chitosan/collagen based biomimetic osteochondral tissue constructs: A growth factor-free approach.
32320808	0	73	theme	biomimetic	24:33	arg1	constructs					56:65	Chitosan/collagen based biomimetic osteochondral tissue constructs	0:65	Chitosan/collagen based biomimetic osteochondral tissue constructs	0:65	Chitosan/collagen based biomimetic osteochondral tissue constructs: A growth factor-free approach.
32320808	1	74	theme	osteochondral	198:210	arg1	constructs					220:229	multi-layered/multi-component osteochondral mimetic constructs	168:229	multi-layered/multi-component osteochondral mimetic constructs	168:229	Tissue engineering approach offers alternative strategies to develop multi-layered/multi-component osteochondral mimetic constructs to meet the requirements of the heterogeneous and layered structure of native osteochondral tissue.
32320808	7	75	theme	osteochondral	1344:1356	arg1	scaffold					1358:1365	the osteochondral scaffold	1340:1365	the osteochondral scaffold	1340:1365	Expression of genes specific for bone, calcified cartilage and cartilage were improved in the osteochondral scaffold.
32320808	1	76	theme	native	302:307	arg1	tissue					323:328	native osteochondral tissue	302:328	native osteochondral tissue	302:328	Tissue engineering approach offers alternative strategies to develop multi-layered/multi-component osteochondral mimetic constructs to meet the requirements of the heterogeneous and layered structure of native osteochondral tissue.
33839232	14	0	theme	novel	2222:2226	arg1	approach					2248:2255	a novel microbiota-targeted approach	2220:2255	a novel microbiota-targeted approach for managing metabolic and neurodegenerative diseases	2220:2309	MOS, as a prebiotics, can be translated into a novel microbiota-targeted approach for managing metabolic and neurodegenerative diseases.
33839232	11	1	theme	reshaped	1645:1652	arg1	microbiome					1658:1667	the reshaped gut microbiome	1641:1667	the reshaped gut microbiome	1641:1667	The correlation analysis indicated that the reshaped gut microbiome and enhanced butyrate formation are highly associated with behavioral alteration and brain oxidative status.
33839232	9	2	theme	gut	1394:1396	arg1	composition					1409:1419	the gut microbiota composition	1390:1419	the gut microbiota composition	1390:1419	MOS re-constructed the gut microbiota composition, including increasing the relative abundance of Lactobacillus and reducing the relative abundance of Helicobacter.
33839232	10	3	theme	related	1576:1582	arg1	microbes					1584:1591	related microbes	1576:1591	related microbes	1576:1591	MOS enhanced the butyrate formation and related microbes levels.
33839232	7	4	theme	HPA-axis	1102:1109	arg1	disorders					1111:1119	the HPA-axis disorders	1098:1119	the HPA-axis disorders	1098:1119	Moreover, MOS also alleviated the HPA-axis disorders by decreasing the levels of hormones corticosterone (CORT) and corticotropin-releasing hormone (CRH) and upregulated the norepinephrine (NE) expressions.
33839232	2	5	theme	gut	294:296	arg1	axis					315:318	The gut microbiota-brain axis	290:318	The gut microbiota-brain axis	290:318	The gut microbiota-brain axis plays a pivotal role during AD development, which could target nutritional intervention.
33839232	4	6	from	w/v	650:652	arg1	water					670:674	the drinking water	657:674	the drinking water	657:674	Here, we found that an 8-week treatment of MOS (0.12%, w/v in the drinking water) significantly improved cognitive function and spatial memory, accompanied by attenuated the anxiety- and obsessive-like behaviors in the 5xFAD transgenic AD mice model.
33839232	4	7	theme	AD	831:832	arg1	model					839:843	the 5xFAD transgenic AD mice model	810:843	the 5xFAD transgenic AD mice model	810:843	Here, we found that an 8-week treatment of MOS (0.12%, w/v in the drinking water) significantly improved cognitive function and spatial memory, accompanied by attenuated the anxiety- and obsessive-like behaviors in the 5xFAD transgenic AD mice model.
33839232	11	8	theme	enhanced	1673:1680	arg1	formation					1691:1699	enhanced butyrate formation	1673:1699	enhanced butyrate formation	1673:1699	The correlation analysis indicated that the reshaped gut microbiome and enhanced butyrate formation are highly associated with behavioral alteration and brain oxidative status.
33839232	3	9	theme	metabolites	550:560	arg1	formation					517:525	the formation	513:525	the formation of the neuroprotective metabolites short-chain fatty acids (SCFAs)	513:592	The prebiotic mannan oligosaccharide (MOS) has been reported to reshape the gut microbiome and enhanced the formation of the neuroprotective metabolites short-chain fatty acids (SCFAs).
33839232	12	10	theme	SCFAs	1778:1782	arg1	experiment					1800:1809	SCFAs supplementation experiment	1778:1809	SCFAs supplementation experiment	1778:1809	SCFAs supplementation experiment also attenuated the behavioral disorders and Aβ accumulation in the AD mice brain, accompanied by balanced HPA-axis and redox status.
33839232	10	11	dep	formation	1562:1570	arg1	levels					1593:1598	levels	1593:1598	levels	1593:1598	MOS enhanced the butyrate formation and related microbes levels.
33839232	0	12	theme	gut	133:135	arg1	axis					154:157	the gut microbiota-brain axis	129:157	the gut microbiota-brain axis	129:157	Mannan oligosaccharide attenuates cognitive and behavioral disorders in the 5xFAD Alzheimer's disease mouse model via regulating the gut microbiota-brain axis.
33839232	0	13	theme	cognitive	34:42	arg1	disorders					59:67	cognitive and behavioral disorders	34:67	cognitive and behavioral disorders	34:67	Mannan oligosaccharide attenuates cognitive and behavioral disorders in the 5xFAD Alzheimer's disease mouse model via regulating the gut microbiota-brain axis.
33839232	9	14	theme	Lactobacillus	1469:1481	arg1	abundance					1456:1464	the relative abundance	1443:1464	the relative abundance of Lactobacillus	1443:1481	MOS re-constructed the gut microbiota composition, including increasing the relative abundance of Lactobacillus and reducing the relative abundance of Helicobacter.
33839232	8	15	theme	gut	1288:1290	arg1	damage					1310:1315	the gut barrier integrity damage	1284:1315	the gut barrier integrity damage	1284:1315	Notably, the gut barrier integrity damage and the LPS leak were prevented by the MOS treatment.
33839232	2	16	theme	microbiota-brain	298:313	arg1	axis					315:318	The gut microbiota-brain axis	290:318	The gut microbiota-brain axis	290:318	The gut microbiota-brain axis plays a pivotal role during AD development, which could target nutritional intervention.
33839232	9	17	theme	relative	1500:1507	arg1	abundance					1509:1517	the relative abundance	1496:1517	the relative abundance of Helicobacter	1496:1533	MOS re-constructed the gut microbiota composition, including increasing the relative abundance of Lactobacillus and reducing the relative abundance of Helicobacter.
33839232	6	18	theme	brain	1001:1005	arg1	status					1013:1018	the brain redox status	997:1018	the brain redox status	997:1018	Importantly, MOS treatment significantly balanced the brain redox status and suppressed the neuroinflammatory responses.
33839232	8	19	theme	integrity	1300:1308	arg1	damage					1310:1315	the gut barrier integrity damage	1284:1315	the gut barrier integrity damage	1284:1315	Notably, the gut barrier integrity damage and the LPS leak were prevented by the MOS treatment.
33839232	13	20	theme	present	1964:1970	arg1	study					1972:1976	the present study	1960:1976	the present study	1960:1976	In conclusion, the present study indicated that MOS significantly attenuates the cognitive and mental deficits in the 5xFAD mice, which could be partly explained by the reshaped microbiome and enhanced SCFAs formation in the gut.
33839232	1	21	theme	psychiatric	268:278	arg1	symptoms					280:287	psychiatric symptoms	268:287	psychiatric symptoms	268:287	Alzheimer's disease (AD) is a progressive neurodegenerative disease characterized by cognitive deficits and psychiatric symptoms.
33839232	5	22	theme	Aβ	876:877	arg1	accumulation					879:890	the Aβ accumulation	872:890	the Aβ accumulation in the cortex, hippocampus, and amygdala of the brain	872:944	MOS substantially reduced the Aβ accumulation in the cortex, hippocampus, and amygdala of the brain.
33839232	7	23	dep	hormones	1149:1156	arg1	corticosterone					1158:1171	corticosterone	1158:1171	corticosterone (CORT)	1158:1178	Moreover, MOS also alleviated the HPA-axis disorders by decreasing the levels of hormones corticosterone (CORT) and corticotropin-releasing hormone (CRH) and upregulated the norepinephrine (NE) expressions.
33839232	7	23	dep	hormones	1149:1156	arg1	hormone					1208:1214	corticotropin-releasing hormone	1184:1214	corticotropin-releasing hormone (CRH)	1184:1220	Moreover, MOS also alleviated the HPA-axis disorders by decreasing the levels of hormones corticosterone (CORT) and corticotropin-releasing hormone (CRH) and upregulated the norepinephrine (NE) expressions.
33839232	7	23	dep	hormones	1149:1156	arg1	CORT					1174:1177	CORT	1174:1177	CORT	1174:1177	Moreover, MOS also alleviated the HPA-axis disorders by decreasing the levels of hormones corticosterone (CORT) and corticotropin-releasing hormone (CRH) and upregulated the norepinephrine (NE) expressions.
33839232	7	23	dep	hormones	1149:1156	arg1	hormones					1149:1156	hormones corticosterone (CORT) and corticotropin-releasing hormone (CRH)	1149:1220	hormones corticosterone (CORT) and corticotropin-releasing hormone (CRH)	1149:1220	Moreover, MOS also alleviated the HPA-axis disorders by decreasing the levels of hormones corticosterone (CORT) and corticotropin-releasing hormone (CRH) and upregulated the norepinephrine (NE) expressions.
33839232	7	23	dep	hormones	1149:1156	arg1	CRH					1217:1219	CRH	1217:1219	CRH	1217:1219	Moreover, MOS also alleviated the HPA-axis disorders by decreasing the levels of hormones corticosterone (CORT) and corticotropin-releasing hormone (CRH) and upregulated the norepinephrine (NE) expressions.
33839232	5	24	from	accumulation	879:890	arg1	amygdala					924:931	amygdala	924:931	amygdala	924:931	MOS substantially reduced the Aβ accumulation in the cortex, hippocampus, and amygdala of the brain.
33839232	5	24	from	accumulation	879:890	arg1	cortex					899:904	cortex	899:904	cortex	899:904	MOS substantially reduced the Aβ accumulation in the cortex, hippocampus, and amygdala of the brain.
33839232	5	24	from	accumulation	879:890	arg1	hippocampus					907:917	hippocampus	907:917	hippocampus	907:917	MOS substantially reduced the Aβ accumulation in the cortex, hippocampus, and amygdala of the brain.
33839232	8	25	theme	LPS	1325:1327	arg1	leak					1329:1332	the LPS leak	1321:1332	the LPS leak	1321:1332	Notably, the gut barrier integrity damage and the LPS leak were prevented by the MOS treatment.
33839232	0	26	theme	disease	94:100	arg1	model					108:112	the 5xFAD Alzheimer's disease mouse model	72:112	the 5xFAD Alzheimer's disease mouse model	72:112	Mannan oligosaccharide attenuates cognitive and behavioral disorders in the 5xFAD Alzheimer's disease mouse model via regulating the gut microbiota-brain axis.
33839232	3	27	theme	fatty	574:578	arg1	SCFAs					587:591	SCFAs	587:591	SCFAs	587:591	The prebiotic mannan oligosaccharide (MOS) has been reported to reshape the gut microbiome and enhanced the formation of the neuroprotective metabolites short-chain fatty acids (SCFAs).
33839232	3	27	theme	fatty	574:578	arg1	acids					580:584	short-chain fatty acids	562:584	the formation of the neuroprotective metabolites short-chain fatty acids (SCFAs)	513:592	The prebiotic mannan oligosaccharide (MOS) has been reported to reshape the gut microbiome and enhanced the formation of the neuroprotective metabolites short-chain fatty acids (SCFAs).
33839232	5	28	theme	brain	940:944	arg1	amygdala					924:931	amygdala	924:931	amygdala	924:931	MOS substantially reduced the Aβ accumulation in the cortex, hippocampus, and amygdala of the brain.
33839232	5	28	theme	brain	940:944	arg1	cortex					899:904	cortex	899:904	cortex	899:904	MOS substantially reduced the Aβ accumulation in the cortex, hippocampus, and amygdala of the brain.
33839232	5	28	theme	brain	940:944	arg1	hippocampus					907:917	hippocampus	907:917	hippocampus	907:917	MOS substantially reduced the Aβ accumulation in the cortex, hippocampus, and amygdala of the brain.
33839232	4	29	theme	attenuated	754:763	arg1	behaviors					797:805	the anxiety- and obsessive-like behaviors	765:805	attenuated the anxiety- and obsessive-like behaviors in the 5xFAD transgenic AD mice model	754:843	Here, we found that an 8-week treatment of MOS (0.12%, w/v in the drinking water) significantly improved cognitive function and spatial memory, accompanied by attenuated the anxiety- and obsessive-like behaviors in the 5xFAD transgenic AD mice model.
33839232	12	30	theme	redox	1931:1935	arg1	status					1937:1942	redox status	1931:1942	redox status	1931:1942	SCFAs supplementation experiment also attenuated the behavioral disorders and Aβ accumulation in the AD mice brain, accompanied by balanced HPA-axis and redox status.
33839232	0	31	theme	Mannan	0:5	arg1	oligosaccharide					7:21	Mannan oligosaccharide	0:21	Mannan oligosaccharide	0:21	Mannan oligosaccharide attenuates cognitive and behavioral disorders in the 5xFAD Alzheimer's disease mouse model via regulating the gut microbiota-brain axis.
33839232	3	32	dep	formation	517:525	arg1	SCFAs					587:591	SCFAs	587:591	SCFAs	587:591	The prebiotic mannan oligosaccharide (MOS) has been reported to reshape the gut microbiome and enhanced the formation of the neuroprotective metabolites short-chain fatty acids (SCFAs).
33839232	3	32	dep	formation	517:525	arg1	acids					580:584	short-chain fatty acids	562:584	the formation of the neuroprotective metabolites short-chain fatty acids (SCFAs)	513:592	The prebiotic mannan oligosaccharide (MOS) has been reported to reshape the gut microbiome and enhanced the formation of the neuroprotective metabolites short-chain fatty acids (SCFAs).
33839232	6	33	theme	neuroinflammatory	1039:1055	arg1	responses					1057:1065	the neuroinflammatory responses	1035:1065	the neuroinflammatory responses	1035:1065	Importantly, MOS treatment significantly balanced the brain redox status and suppressed the neuroinflammatory responses.
33839232	8	34	theme	MOS	1356:1358	arg1	treatment					1360:1368	the MOS treatment	1352:1368	the MOS treatment	1352:1368	Notably, the gut barrier integrity damage and the LPS leak were prevented by the MOS treatment.
33839232	11	35	theme	behavioral	1728:1737	arg1	alteration					1739:1748	behavioral alteration	1728:1748	behavioral alteration	1728:1748	The correlation analysis indicated that the reshaped gut microbiome and enhanced butyrate formation are highly associated with behavioral alteration and brain oxidative status.
33839232	4	36	dep	MOS	638:640	arg1	%					647:647	0.12%	643:647	0.12%	643:647	Here, we found that an 8-week treatment of MOS (0.12%, w/v in the drinking water) significantly improved cognitive function and spatial memory, accompanied by attenuated the anxiety- and obsessive-like behaviors in the 5xFAD transgenic AD mice model.
33839232	4	36	dep	MOS	638:640	arg1	w/v					650:652	w/v	650:652	w/v in the drinking water	650:674	Here, we found that an 8-week treatment of MOS (0.12%, w/v in the drinking water) significantly improved cognitive function and spatial memory, accompanied by attenuated the anxiety- and obsessive-like behaviors in the 5xFAD transgenic AD mice model.
33839232	3	37	theme	prebiotic	413:421	arg1	MOS					447:449	MOS	447:449	MOS	447:449	The prebiotic mannan oligosaccharide (MOS) has been reported to reshape the gut microbiome and enhanced the formation of the neuroprotective metabolites short-chain fatty acids (SCFAs).
33839232	3	37	theme	prebiotic	413:421	arg1	oligosaccharide					430:444	The prebiotic mannan oligosaccharide	409:444	The prebiotic mannan oligosaccharide (MOS)	409:450	The prebiotic mannan oligosaccharide (MOS) has been reported to reshape the gut microbiome and enhanced the formation of the neuroprotective metabolites short-chain fatty acids (SCFAs).
33839232	4	38	theme	5xFAD	814:818	arg1	model					839:843	the 5xFAD transgenic AD mice model	810:843	the 5xFAD transgenic AD mice model	810:843	Here, we found that an 8-week treatment of MOS (0.12%, w/v in the drinking water) significantly improved cognitive function and spatial memory, accompanied by attenuated the anxiety- and obsessive-like behaviors in the 5xFAD transgenic AD mice model.
33839232	11	39	theme	correlation	1605:1615	arg1	analysis					1617:1624	The correlation analysis	1601:1624	The correlation analysis	1601:1624	The correlation analysis indicated that the reshaped gut microbiome and enhanced butyrate formation are highly associated with behavioral alteration and brain oxidative status.
33839232	12	40	theme	mice	1882:1885	arg1	brain					1887:1891	the AD mice brain	1875:1891	the AD mice brain	1875:1891	SCFAs supplementation experiment also attenuated the behavioral disorders and Aβ accumulation in the AD mice brain, accompanied by balanced HPA-axis and redox status.
33839232	12	41	theme	AD	1879:1880	arg1	brain					1887:1891	the AD mice brain	1875:1891	the AD mice brain	1875:1891	SCFAs supplementation experiment also attenuated the behavioral disorders and Aβ accumulation in the AD mice brain, accompanied by balanced HPA-axis and redox status.
33839232	11	42	theme	oxidative	1760:1768	arg1	status					1770:1775	brain oxidative status	1754:1775	brain oxidative status	1754:1775	The correlation analysis indicated that the reshaped gut microbiome and enhanced butyrate formation are highly associated with behavioral alteration and brain oxidative status.
33839232	4	43	theme	anxiety-	769:776	arg1	behaviors					797:805	the anxiety- and obsessive-like behaviors	765:805	attenuated the anxiety- and obsessive-like behaviors in the 5xFAD transgenic AD mice model	754:843	Here, we found that an 8-week treatment of MOS (0.12%, w/v in the drinking water) significantly improved cognitive function and spatial memory, accompanied by attenuated the anxiety- and obsessive-like behaviors in the 5xFAD transgenic AD mice model.
33839232	3	44	theme	gut	485:487	arg1	microbiome					489:498	the gut microbiome	481:498	the gut microbiome	481:498	The prebiotic mannan oligosaccharide (MOS) has been reported to reshape the gut microbiome and enhanced the formation of the neuroprotective metabolites short-chain fatty acids (SCFAs).
33839232	10	45	theme	butyrate	1553:1560	arg1	formation					1562:1570	the butyrate formation	1549:1570	the butyrate formation	1549:1570	MOS enhanced the butyrate formation and related microbes levels.
33839232	4	46	theme	obsessive-like	782:795	arg1	behaviors					797:805	the anxiety- and obsessive-like behaviors	765:805	attenuated the anxiety- and obsessive-like behaviors in the 5xFAD transgenic AD mice model	754:843	Here, we found that an 8-week treatment of MOS (0.12%, w/v in the drinking water) significantly improved cognitive function and spatial memory, accompanied by attenuated the anxiety- and obsessive-like behaviors in the 5xFAD transgenic AD mice model.
33839232	13	47	theme	mental	2040:2045	arg1	deficits					2047:2054	the cognitive and mental deficits	2022:2054	the cognitive and mental deficits	2022:2054	In conclusion, the present study indicated that MOS significantly attenuates the cognitive and mental deficits in the 5xFAD mice, which could be partly explained by the reshaped microbiome and enhanced SCFAs formation in the gut.
33839232	12	48	theme	Aβ	1856:1857	arg1	accumulation					1859:1870	Aβ accumulation	1856:1870	Aβ accumulation	1856:1870	SCFAs supplementation experiment also attenuated the behavioral disorders and Aβ accumulation in the AD mice brain, accompanied by balanced HPA-axis and redox status.
33839232	14	49	theme	microbiota-targeted	2228:2246	arg1	approach					2248:2255	a novel microbiota-targeted approach	2220:2255	a novel microbiota-targeted approach for managing metabolic and neurodegenerative diseases	2220:2309	MOS, as a prebiotics, can be translated into a novel microbiota-targeted approach for managing metabolic and neurodegenerative diseases.
33839232	11	50	theme	gut	1654:1656	arg1	microbiome					1658:1667	the reshaped gut microbiome	1641:1667	the reshaped gut microbiome	1641:1667	The correlation analysis indicated that the reshaped gut microbiome and enhanced butyrate formation are highly associated with behavioral alteration and brain oxidative status.
33839232	3	51	theme	short-chain	562:572	arg1	SCFAs					587:591	SCFAs	587:591	SCFAs	587:591	The prebiotic mannan oligosaccharide (MOS) has been reported to reshape the gut microbiome and enhanced the formation of the neuroprotective metabolites short-chain fatty acids (SCFAs).
33839232	3	51	theme	short-chain	562:572	arg1	acids					580:584	short-chain fatty acids	562:584	the formation of the neuroprotective metabolites short-chain fatty acids (SCFAs)	513:592	The prebiotic mannan oligosaccharide (MOS) has been reported to reshape the gut microbiome and enhanced the formation of the neuroprotective metabolites short-chain fatty acids (SCFAs).
33839232	9	52	theme	microbiota	1398:1407	arg1	composition					1409:1419	the gut microbiota composition	1390:1419	the gut microbiota composition	1390:1419	MOS re-constructed the gut microbiota composition, including increasing the relative abundance of Lactobacillus and reducing the relative abundance of Helicobacter.
33839232	13	53	theme	5xFAD	2063:2067	arg1	mice					2069:2072	the 5xFAD mice	2059:2072	the 5xFAD mice	2059:2072	In conclusion, the present study indicated that MOS significantly attenuates the cognitive and mental deficits in the 5xFAD mice, which could be partly explained by the reshaped microbiome and enhanced SCFAs formation in the gut.
33839232	13	54	theme	cognitive	2026:2034	arg1	deficits					2047:2054	the cognitive and mental deficits	2022:2054	the cognitive and mental deficits	2022:2054	In conclusion, the present study indicated that MOS significantly attenuates the cognitive and mental deficits in the 5xFAD mice, which could be partly explained by the reshaped microbiome and enhanced SCFAs formation in the gut.
33839232	3	55	theme	neuroprotective	534:548	arg1	metabolites					550:560	the neuroprotective metabolites	530:560	the neuroprotective metabolites	530:560	The prebiotic mannan oligosaccharide (MOS) has been reported to reshape the gut microbiome and enhanced the formation of the neuroprotective metabolites short-chain fatty acids (SCFAs).
33839232	13	56	from	deficits	2047:2054	arg1	mice					2069:2072	the 5xFAD mice	2059:2072	the 5xFAD mice	2059:2072	In conclusion, the present study indicated that MOS significantly attenuates the cognitive and mental deficits in the 5xFAD mice, which could be partly explained by the reshaped microbiome and enhanced SCFAs formation in the gut.
33839232	4	57	theme	transgenic	820:829	arg1	model					839:843	the 5xFAD transgenic AD mice model	810:843	the 5xFAD transgenic AD mice model	810:843	Here, we found that an 8-week treatment of MOS (0.12%, w/v in the drinking water) significantly improved cognitive function and spatial memory, accompanied by attenuated the anxiety- and obsessive-like behaviors in the 5xFAD transgenic AD mice model.
33839232	11	58	theme	butyrate	1682:1689	arg1	formation					1691:1699	enhanced butyrate formation	1673:1699	enhanced butyrate formation	1673:1699	The correlation analysis indicated that the reshaped gut microbiome and enhanced butyrate formation are highly associated with behavioral alteration and brain oxidative status.
33839232	2	59	theme	pivotal	328:334	arg1	role					336:339	a pivotal role	326:339	a pivotal role	326:339	The gut microbiota-brain axis plays a pivotal role during AD development, which could target nutritional intervention.
33839232	4	60	theme	mice	834:837	arg1	model					839:843	the 5xFAD transgenic AD mice model	810:843	the 5xFAD transgenic AD mice model	810:843	Here, we found that an 8-week treatment of MOS (0.12%, w/v in the drinking water) significantly improved cognitive function and spatial memory, accompanied by attenuated the anxiety- and obsessive-like behaviors in the 5xFAD transgenic AD mice model.
33839232	12	61	theme	supplementation	1784:1798	arg1	experiment					1800:1809	SCFAs supplementation experiment	1778:1809	SCFAs supplementation experiment	1778:1809	SCFAs supplementation experiment also attenuated the behavioral disorders and Aβ accumulation in the AD mice brain, accompanied by balanced HPA-axis and redox status.
33839232	9	62	theme	relative	1447:1454	arg1	abundance					1456:1464	the relative abundance	1443:1464	the relative abundance of Lactobacillus	1443:1481	MOS re-constructed the gut microbiota composition, including increasing the relative abundance of Lactobacillus and reducing the relative abundance of Helicobacter.
33839232	0	63	theme	microbiota-brain	137:152	arg1	axis					154:157	the gut microbiota-brain axis	129:157	the gut microbiota-brain axis	129:157	Mannan oligosaccharide attenuates cognitive and behavioral disorders in the 5xFAD Alzheimer's disease mouse model via regulating the gut microbiota-brain axis.
33839232	1	64	theme	progressive	190:200	arg1	disease					220:226	a progressive neurodegenerative disease	188:226	a progressive neurodegenerative disease characterized by cognitive deficits and psychiatric symptoms	188:287	Alzheimer's disease (AD) is a progressive neurodegenerative disease characterized by cognitive deficits and psychiatric symptoms.
33839232	1	64	theme	progressive	190:200	arg1	disease					172:178	Alzheimer's disease	160:178	Alzheimer's disease (AD)	160:183	Alzheimer's disease (AD) is a progressive neurodegenerative disease characterized by cognitive deficits and psychiatric symptoms.
33839232	4	65	theme	MOS	638:640	arg1	treatment					625:633	an 8-week treatment	615:633	an 8-week treatment of MOS (0.12%, w/v in the drinking water)	615:675	Here, we found that an 8-week treatment of MOS (0.12%, w/v in the drinking water) significantly improved cognitive function and spatial memory, accompanied by attenuated the anxiety- and obsessive-like behaviors in the 5xFAD transgenic AD mice model.
33839232	7	66	theme	corticotropin-releasing	1184:1206	arg1	CRH					1217:1219	CRH	1217:1219	CRH	1217:1219	Moreover, MOS also alleviated the HPA-axis disorders by decreasing the levels of hormones corticosterone (CORT) and corticotropin-releasing hormone (CRH) and upregulated the norepinephrine (NE) expressions.
33839232	7	66	theme	corticotropin-releasing	1184:1206	arg1	hormone					1208:1214	corticotropin-releasing hormone	1184:1214	corticotropin-releasing hormone (CRH)	1184:1220	Moreover, MOS also alleviated the HPA-axis disorders by decreasing the levels of hormones corticosterone (CORT) and corticotropin-releasing hormone (CRH) and upregulated the norepinephrine (NE) expressions.
33839232	7	66	theme	corticotropin-releasing	1184:1206	arg1	hormones					1149:1156	hormones corticosterone (CORT) and corticotropin-releasing hormone (CRH)	1149:1220	hormones corticosterone (CORT) and corticotropin-releasing hormone (CRH)	1149:1220	Moreover, MOS also alleviated the HPA-axis disorders by decreasing the levels of hormones corticosterone (CORT) and corticotropin-releasing hormone (CRH) and upregulated the norepinephrine (NE) expressions.
33839232	4	67	from	behaviors	797:805	arg1	model					839:843	the 5xFAD transgenic AD mice model	810:843	the 5xFAD transgenic AD mice model	810:843	Here, we found that an 8-week treatment of MOS (0.12%, w/v in the drinking water) significantly improved cognitive function and spatial memory, accompanied by attenuated the anxiety- and obsessive-like behaviors in the 5xFAD transgenic AD mice model.
33839232	6	68	theme	redox	1007:1011	arg1	status					1013:1018	the brain redox status	997:1018	the brain redox status	997:1018	Importantly, MOS treatment significantly balanced the brain redox status and suppressed the neuroinflammatory responses.
33839232	4	69	theme	cognitive	700:708	arg1	function					710:717	cognitive function	700:717	cognitive function	700:717	Here, we found that an 8-week treatment of MOS (0.12%, w/v in the drinking water) significantly improved cognitive function and spatial memory, accompanied by attenuated the anxiety- and obsessive-like behaviors in the 5xFAD transgenic AD mice model.
33839232	0	70	theme	5xFAD	76:80	arg1	model					108:112	the 5xFAD Alzheimer's disease mouse model	72:112	the 5xFAD Alzheimer's disease mouse model	72:112	Mannan oligosaccharide attenuates cognitive and behavioral disorders in the 5xFAD Alzheimer's disease mouse model via regulating the gut microbiota-brain axis.
33839232	13	71	theme	SCFAs	2147:2151	arg1	formation					2153:2161	SCFAs formation	2147:2161	SCFAs formation	2147:2161	In conclusion, the present study indicated that MOS significantly attenuates the cognitive and mental deficits in the 5xFAD mice, which could be partly explained by the reshaped microbiome and enhanced SCFAs formation in the gut.
33839232	8	72	theme	barrier	1292:1298	arg1	damage					1310:1315	the gut barrier integrity damage	1284:1315	the gut barrier integrity damage	1284:1315	Notably, the gut barrier integrity damage and the LPS leak were prevented by the MOS treatment.
33839232	9	73	theme	Helicobacter	1522:1533	arg1	abundance					1509:1517	the relative abundance	1496:1517	the relative abundance of Helicobacter	1496:1533	MOS re-constructed the gut microbiota composition, including increasing the relative abundance of Lactobacillus and reducing the relative abundance of Helicobacter.
33839232	4	74	theme	drinking	661:668	arg1	water					670:674	the drinking water	657:674	the drinking water	657:674	Here, we found that an 8-week treatment of MOS (0.12%, w/v in the drinking water) significantly improved cognitive function and spatial memory, accompanied by attenuated the anxiety- and obsessive-like behaviors in the 5xFAD transgenic AD mice model.
33839232	0	75	theme	mouse	102:106	arg1	model					108:112	the 5xFAD Alzheimer's disease mouse model	72:112	the 5xFAD Alzheimer's disease mouse model	72:112	Mannan oligosaccharide attenuates cognitive and behavioral disorders in the 5xFAD Alzheimer's disease mouse model via regulating the gut microbiota-brain axis.
33839232	13	76	theme	reshaped	2114:2121	arg1	microbiome					2123:2132	the reshaped microbiome	2110:2132	the reshaped microbiome	2110:2132	In conclusion, the present study indicated that MOS significantly attenuates the cognitive and mental deficits in the 5xFAD mice, which could be partly explained by the reshaped microbiome and enhanced SCFAs formation in the gut.
33839232	6	77	theme	MOS	960:962	arg1	treatment					964:972	MOS treatment	960:972	MOS treatment	960:972	Importantly, MOS treatment significantly balanced the brain redox status and suppressed the neuroinflammatory responses.
33839232	14	78	theme	neurodegenerative	2284:2300	arg1	diseases					2302:2309	metabolic and neurodegenerative diseases	2270:2309	metabolic and neurodegenerative diseases	2270:2309	MOS, as a prebiotics, can be translated into a novel microbiota-targeted approach for managing metabolic and neurodegenerative diseases.
33839232	2	79	theme	nutritional	383:393	arg1	intervention					395:406	nutritional intervention	383:406	nutritional intervention	383:406	The gut microbiota-brain axis plays a pivotal role during AD development, which could target nutritional intervention.
33839232	7	80	theme	hormones	1149:1156	arg1	levels					1139:1144	the levels	1135:1144	the levels of hormones corticosterone (CORT) and corticotropin-releasing hormone (CRH)	1135:1220	Moreover, MOS also alleviated the HPA-axis disorders by decreasing the levels of hormones corticosterone (CORT) and corticotropin-releasing hormone (CRH) and upregulated the norepinephrine (NE) expressions.
33839232	12	81	theme	balanced	1909:1916	arg1	HPA-axis					1918:1925	balanced HPA-axis	1909:1925	balanced HPA-axis	1909:1925	SCFAs supplementation experiment also attenuated the behavioral disorders and Aβ accumulation in the AD mice brain, accompanied by balanced HPA-axis and redox status.
33839232	4	82	theme	spatial	723:729	arg1	memory					731:736	spatial memory	723:736	spatial memory	723:736	Here, we found that an 8-week treatment of MOS (0.12%, w/v in the drinking water) significantly improved cognitive function and spatial memory, accompanied by attenuated the anxiety- and obsessive-like behaviors in the 5xFAD transgenic AD mice model.
33839232	14	83	theme	metabolic	2270:2278	arg1	diseases					2302:2309	metabolic and neurodegenerative diseases	2270:2309	metabolic and neurodegenerative diseases	2270:2309	MOS, as a prebiotics, can be translated into a novel microbiota-targeted approach for managing metabolic and neurodegenerative diseases.
33839232	0	84	theme	behavioral	48:57	arg1	disorders					59:67	cognitive and behavioral disorders	34:67	cognitive and behavioral disorders	34:67	Mannan oligosaccharide attenuates cognitive and behavioral disorders in the 5xFAD Alzheimer's disease mouse model via regulating the gut microbiota-brain axis.
33839232	3	85	theme	mannan	423:428	arg1	MOS					447:449	MOS	447:449	MOS	447:449	The prebiotic mannan oligosaccharide (MOS) has been reported to reshape the gut microbiome and enhanced the formation of the neuroprotective metabolites short-chain fatty acids (SCFAs).
33839232	3	85	theme	mannan	423:428	arg1	oligosaccharide					430:444	The prebiotic mannan oligosaccharide	409:444	The prebiotic mannan oligosaccharide (MOS)	409:450	The prebiotic mannan oligosaccharide (MOS) has been reported to reshape the gut microbiome and enhanced the formation of the neuroprotective metabolites short-chain fatty acids (SCFAs).
33839232	11	86	theme	brain	1754:1758	arg1	status					1770:1775	brain oxidative status	1754:1775	brain oxidative status	1754:1775	The correlation analysis indicated that the reshaped gut microbiome and enhanced butyrate formation are highly associated with behavioral alteration and brain oxidative status.
33839232	2	87	theme	AD	348:349	arg1	development					351:361	AD development	348:361	AD development	348:361	The gut microbiota-brain axis plays a pivotal role during AD development, which could target nutritional intervention.
33839232	1	88	theme	neurodegenerative	202:218	arg1	disease					220:226	a progressive neurodegenerative disease	188:226	a progressive neurodegenerative disease characterized by cognitive deficits and psychiatric symptoms	188:287	Alzheimer's disease (AD) is a progressive neurodegenerative disease characterized by cognitive deficits and psychiatric symptoms.
33839232	1	88	theme	neurodegenerative	202:218	arg1	disease					172:178	Alzheimer's disease	160:178	Alzheimer's disease (AD)	160:183	Alzheimer's disease (AD) is a progressive neurodegenerative disease characterized by cognitive deficits and psychiatric symptoms.
33839232	12	89	theme	behavioral	1831:1840	arg1	disorders					1842:1850	the behavioral disorders	1827:1850	the behavioral disorders	1827:1850	SCFAs supplementation experiment also attenuated the behavioral disorders and Aβ accumulation in the AD mice brain, accompanied by balanced HPA-axis and redox status.
33839232	7	90	theme	norepinephrine	1242:1255	arg1	expressions					1262:1272	the norepinephrine (NE) expressions	1238:1272	the norepinephrine (NE) expressions	1238:1272	Moreover, MOS also alleviated the HPA-axis disorders by decreasing the levels of hormones corticosterone (CORT) and corticotropin-releasing hormone (CRH) and upregulated the norepinephrine (NE) expressions.
33839232	4	91	theme	8-week	618:623	arg1	treatment					625:633	an 8-week treatment	615:633	an 8-week treatment of MOS (0.12%, w/v in the drinking water)	615:675	Here, we found that an 8-week treatment of MOS (0.12%, w/v in the drinking water) significantly improved cognitive function and spatial memory, accompanied by attenuated the anxiety- and obsessive-like behaviors in the 5xFAD transgenic AD mice model.
33839232	1	92	theme	cognitive	245:253	arg1	deficits					255:262	cognitive deficits	245:262	cognitive deficits	245:262	Alzheimer's disease (AD) is a progressive neurodegenerative disease characterized by cognitive deficits and psychiatric symptoms.
33609936	7	0	theme	biocompatibility	1292:1307	arg1	studies					1309:1315	The in-vivo biocompatibility studies	1280:1315	The in-vivo biocompatibility studies for 10 days	1280:1327	The in-vivo biocompatibility studies for 10 days revealed that composite system is non-toxic in nature.
33609936	2	1	theme	initial	325:331	arg1	release					344:350	initial burst drug release	325:350	initial burst drug release	325:350	Nonetheless, owing to initial burst drug release, non-specific biodistribution and low retention time at the target site in body, these polymeric drug delivery systems have been found with low in-vivo performance.
33609936	4	2	theme	novel	666:670	arg1	system					767:772	a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system	664:772	a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system	664:772	Herein, a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system has been developed for the treatment of inflammatory arthritis.
33609936	8	3	from	inflammation	1475:1486	arg1	rats					1528:1531	wistar rats	1521:1531	wistar rats	1521:1531	The developed MTX-MPs-H composite drug delivery system effectively decreased the swelling/ inflammation of the arthritis affected paw in wistar rats in comparison to only alginate microparticles and pure MTX up to 30 days.
33609936	4	4	theme	injectable	682:691	arg1	system					767:772	a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system	664:772	a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system	664:772	Herein, a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system has been developed for the treatment of inflammatory arthritis.
33609936	3	5	theme	drug	593:596	arg1	need					635:638	the current need	623:638	the current need in biomedicine	623:653	Hence, the above limitations need to be resolved by designing a smart novel drug delivery system which is the current need in biomedicine.
33609936	3	5	theme	drug	593:596	arg1	system					607:612	a smart novel drug delivery system	579:612	a smart novel drug delivery system which is the current need in biomedicine	579:653	Hence, the above limitations need to be resolved by designing a smart novel drug delivery system which is the current need in biomedicine.
33609936	0	6	theme	arthritis	148:156	arg1	treatment					158:166	inflammatory arthritis treatment	135:166	inflammatory arthritis treatment	135:166	Synthesis and characterization of an injectable microparticles integrated hydrogel composite biomaterial: In-vivo biocompatibility and inflammatory arthritis treatment.
33609936	3	7	theme	smart	581:585	arg1	need					635:638	the current need	623:638	the current need in biomedicine	623:653	Hence, the above limitations need to be resolved by designing a smart novel drug delivery system which is the current need in biomedicine.
33609936	3	7	theme	smart	581:585	arg1	system					607:612	a smart novel drug delivery system	579:612	a smart novel drug delivery system which is the current need in biomedicine	579:653	Hence, the above limitations need to be resolved by designing a smart novel drug delivery system which is the current need in biomedicine.
33609936	1	8	theme	drug	244:247	arg1	applications					258:269	localized drug delivery applications	234:269	localized drug delivery applications for the treatment of arthritis	234:300	Polymeric hydrogels and microparticles have been widely used for localized drug delivery applications for the treatment of arthritis.
33609936	8	9	theme	wistar	1521:1526	arg1	rats					1528:1531	wistar rats	1521:1531	wistar rats	1521:1531	The developed MTX-MPs-H composite drug delivery system effectively decreased the swelling/ inflammation of the arthritis affected paw in wistar rats in comparison to only alginate microparticles and pure MTX up to 30 days.
33609936	5	10	theme	loaded	879:884	arg1	microparticles					895:908	methotrexate (MTX) loaded alginate microparticles	860:908	methotrexate (MTX) loaded alginate microparticles (MTX-Microparticles)	860:929	In the current study, methotrexate (MTX) loaded alginate microparticles (MTX-Microparticles) are embedded into thermoreversible hydrogel matrix (MTX-MPs-H) prepared by physical blending of sodium hyaluronate and methylcellulose (SHMC).
33609936	5	10	theme	loaded	879:884	arg1	MTX-Microparticles					911:928	MTX-Microparticles	911:928	MTX-Microparticles	911:928	In the current study, methotrexate (MTX) loaded alginate microparticles (MTX-Microparticles) are embedded into thermoreversible hydrogel matrix (MTX-MPs-H) prepared by physical blending of sodium hyaluronate and methylcellulose (SHMC).
33609936	4	11	theme	drug	753:756	arg1	system					767:772	a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system	664:772	a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system	664:772	Herein, a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system has been developed for the treatment of inflammatory arthritis.
33609936	5	12	theme	hydrogel	966:973	arg1	MTX-MPs-H					983:991	MTX-MPs-H	983:991	MTX-MPs-H	983:991	In the current study, methotrexate (MTX) loaded alginate microparticles (MTX-Microparticles) are embedded into thermoreversible hydrogel matrix (MTX-MPs-H) prepared by physical blending of sodium hyaluronate and methylcellulose (SHMC).
33609936	5	12	theme	hydrogel	966:973	arg1	matrix					975:980	thermoreversible hydrogel matrix	949:980	thermoreversible hydrogel matrix (MTX-MPs-H) prepared by physical blending of sodium hyaluronate and methylcellulose (SHMC)	949:1071	In the current study, methotrexate (MTX) loaded alginate microparticles (MTX-Microparticles) are embedded into thermoreversible hydrogel matrix (MTX-MPs-H) prepared by physical blending of sodium hyaluronate and methylcellulose (SHMC).
33609936	6	13	theme	release	1263:1269	arg1	profile					1271:1277	controlled drug release profile	1247:1277	controlled drug release profile	1247:1277	Microparticles-hydrogel composite system exhibited appropriate in-vitro thermoreversibility (sol at 4 °C and gel at 37 °C), biocompatibility (>80 %), hemocompatibility, and controlled drug release profile.
33609936	1	14	theme	Polymeric	169:177	arg1	hydrogels					179:187	Polymeric hydrogels	169:187	Polymeric hydrogels	169:187	Polymeric hydrogels and microparticles have been widely used for localized drug delivery applications for the treatment of arthritis.
33609936	8	15	theme	alginate	1555:1562	arg1	microparticles					1564:1577	only alginate microparticles	1550:1577	only alginate microparticles	1550:1577	The developed MTX-MPs-H composite drug delivery system effectively decreased the swelling/ inflammation of the arthritis affected paw in wistar rats in comparison to only alginate microparticles and pure MTX up to 30 days.
33609936	6	16	theme	controlled	1247:1256	arg1	profile					1271:1277	controlled drug release profile	1247:1277	controlled drug release profile	1247:1277	Microparticles-hydrogel composite system exhibited appropriate in-vitro thermoreversibility (sol at 4 °C and gel at 37 °C), biocompatibility (>80 %), hemocompatibility, and controlled drug release profile.
33609936	4	17	theme	microparticles	710:723	arg1	system					767:772	a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system	664:772	a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system	664:772	Herein, a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system has been developed for the treatment of inflammatory arthritis.
33609936	0	18	theme	hydrogel	74:81	arg1	biomaterial					93:103	integrated hydrogel composite biomaterial	63:103	integrated hydrogel composite biomaterial: In-vivo biocompatibility and inflammatory arthritis treatment	63:166	Synthesis and characterization of an injectable microparticles integrated hydrogel composite biomaterial: In-vivo biocompatibility and inflammatory arthritis treatment.
33609936	3	19	theme	current	627:633	arg1	need					635:638	the current need	623:638	the current need in biomedicine	623:653	Hence, the above limitations need to be resolved by designing a smart novel drug delivery system which is the current need in biomedicine.
33609936	3	19	theme	current	627:633	arg1	system					607:612	a smart novel drug delivery system	579:612	a smart novel drug delivery system which is the current need in biomedicine	579:653	Hence, the above limitations need to be resolved by designing a smart novel drug delivery system which is the current need in biomedicine.
33609936	4	20	theme	hydrogel	734:741	arg1	system					767:772	a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system	664:772	a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system	664:772	Herein, a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system has been developed for the treatment of inflammatory arthritis.
33609936	5	21	from	embedded	935:942	arg1	study					853:857	the current study	841:857	the current study	841:857	In the current study, methotrexate (MTX) loaded alginate microparticles (MTX-Microparticles) are embedded into thermoreversible hydrogel matrix (MTX-MPs-H) prepared by physical blending of sodium hyaluronate and methylcellulose (SHMC).
33609936	1	22	used	used	225:228	arg2	microparticles					193:206	microparticles	193:206	microparticles	193:206	Polymeric hydrogels and microparticles have been widely used for localized drug delivery applications for the treatment of arthritis.
33609936	1	22	used	used	225:228	arg2	hydrogels					179:187	Polymeric hydrogels	169:187	Polymeric hydrogels	169:187	Polymeric hydrogels and microparticles have been widely used for localized drug delivery applications for the treatment of arthritis.
33609936	8	23	theme	composite	1408:1416	arg1	system					1432:1437	The developed MTX-MPs-H composite drug delivery system	1384:1437	The developed MTX-MPs-H composite drug delivery system	1384:1437	The developed MTX-MPs-H composite drug delivery system effectively decreased the swelling/ inflammation of the arthritis affected paw in wistar rats in comparison to only alginate microparticles and pure MTX up to 30 days.
33609936	0	24	theme	In-vivo	106:112	arg1	biocompatibility					114:129	In-vivo biocompatibility	106:129	In-vivo biocompatibility	106:129	Synthesis and characterization of an injectable microparticles integrated hydrogel composite biomaterial: In-vivo biocompatibility and inflammatory arthritis treatment.
33609936	8	25	theme	delivery	1423:1430	arg1	system					1432:1437	The developed MTX-MPs-H composite drug delivery system	1384:1437	The developed MTX-MPs-H composite drug delivery system	1384:1437	The developed MTX-MPs-H composite drug delivery system effectively decreased the swelling/ inflammation of the arthritis affected paw in wistar rats in comparison to only alginate microparticles and pure MTX up to 30 days.
33609936	5	26	theme	sodium	1027:1032	arg1	hyaluronate					1034:1044	sodium hyaluronate	1027:1044	sodium hyaluronate	1027:1044	In the current study, methotrexate (MTX) loaded alginate microparticles (MTX-Microparticles) are embedded into thermoreversible hydrogel matrix (MTX-MPs-H) prepared by physical blending of sodium hyaluronate and methylcellulose (SHMC).
33609936	6	27	dep	thermoreversibility	1146:1164	arg1	sol					1167:1169	sol	1167:1169	sol	1167:1169	Microparticles-hydrogel composite system exhibited appropriate in-vitro thermoreversibility (sol at 4 °C and gel at 37 °C), biocompatibility (>80 %), hemocompatibility, and controlled drug release profile.
33609936	8	28	dep	microparticles	1564:1577	arg1	comparison					1536:1545	comparison	1536:1545	comparison	1536:1545	The developed MTX-MPs-H composite drug delivery system effectively decreased the swelling/ inflammation of the arthritis affected paw in wistar rats in comparison to only alginate microparticles and pure MTX up to 30 days.
33609936	0	29	dep	biomaterial	93:103	arg1	biocompatibility					114:129	In-vivo biocompatibility	106:129	In-vivo biocompatibility	106:129	Synthesis and characterization of an injectable microparticles integrated hydrogel composite biomaterial: In-vivo biocompatibility and inflammatory arthritis treatment.
33609936	0	29	dep	biomaterial	93:103	arg1	treatment					158:166	inflammatory arthritis treatment	135:166	inflammatory arthritis treatment	135:166	Synthesis and characterization of an injectable microparticles integrated hydrogel composite biomaterial: In-vivo biocompatibility and inflammatory arthritis treatment.
33609936	6	30	theme	in-vitro	1137:1144	arg1	thermoreversibility					1146:1164	appropriate in-vitro thermoreversibility	1125:1164	appropriate in-vitro thermoreversibility (sol at 4 °C and gel at 37 °C)	1125:1195	Microparticles-hydrogel composite system exhibited appropriate in-vitro thermoreversibility (sol at 4 °C and gel at 37 °C), biocompatibility (>80 %), hemocompatibility, and controlled drug release profile.
33609936	2	31	theme	delivery	454:461	arg1	systems					463:469	these polymeric drug delivery systems	433:469	these polymeric drug delivery systems	433:469	Nonetheless, owing to initial burst drug release, non-specific biodistribution and low retention time at the target site in body, these polymeric drug delivery systems have been found with low in-vivo performance.
33609936	5	32	theme	methotrexate	860:871	arg1	microparticles					895:908	methotrexate (MTX) loaded alginate microparticles	860:908	methotrexate (MTX) loaded alginate microparticles (MTX-Microparticles)	860:929	In the current study, methotrexate (MTX) loaded alginate microparticles (MTX-Microparticles) are embedded into thermoreversible hydrogel matrix (MTX-MPs-H) prepared by physical blending of sodium hyaluronate and methylcellulose (SHMC).
33609936	5	32	theme	methotrexate	860:871	arg1	MTX-Microparticles					911:928	MTX-Microparticles	911:928	MTX-Microparticles	911:928	In the current study, methotrexate (MTX) loaded alginate microparticles (MTX-Microparticles) are embedded into thermoreversible hydrogel matrix (MTX-MPs-H) prepared by physical blending of sodium hyaluronate and methylcellulose (SHMC).
33609936	2	33	theme	polymeric	439:447	arg1	systems					463:469	these polymeric drug delivery systems	433:469	these polymeric drug delivery systems	433:469	Nonetheless, owing to initial burst drug release, non-specific biodistribution and low retention time at the target site in body, these polymeric drug delivery systems have been found with low in-vivo performance.
33609936	6	34	theme	composite	1098:1106	arg1	system					1108:1113	Microparticles-hydrogel composite system	1074:1113	Microparticles-hydrogel composite system	1074:1113	Microparticles-hydrogel composite system exhibited appropriate in-vitro thermoreversibility (sol at 4 °C and gel at 37 °C), biocompatibility (>80 %), hemocompatibility, and controlled drug release profile.
33609936	2	35	theme	low	492:494	arg1	performance					504:514	low in-vivo performance	492:514	low in-vivo performance	492:514	Nonetheless, owing to initial burst drug release, non-specific biodistribution and low retention time at the target site in body, these polymeric drug delivery systems have been found with low in-vivo performance.
33609936	7	36	theme	in-vivo	1284:1290	arg1	studies					1309:1315	The in-vivo biocompatibility studies	1280:1315	The in-vivo biocompatibility studies for 10 days	1280:1327	The in-vivo biocompatibility studies for 10 days revealed that composite system is non-toxic in nature.
33609936	2	37	theme	retention	390:398	arg1	time					400:403	low retention time	386:403	low retention time	386:403	Nonetheless, owing to initial burst drug release, non-specific biodistribution and low retention time at the target site in body, these polymeric drug delivery systems have been found with low in-vivo performance.
33609936	7	38	from	non-toxic	1363:1371	arg1	nature					1376:1381	nature	1376:1381	nature	1376:1381	The in-vivo biocompatibility studies for 10 days revealed that composite system is non-toxic in nature.
33609936	7	39	theme	composite	1343:1351	arg1	system					1353:1358	composite system	1343:1358	composite system	1343:1358	The in-vivo biocompatibility studies for 10 days revealed that composite system is non-toxic in nature.
33609936	8	40	theme	affected	1505:1512	arg1	paw					1514:1516	the arthritis affected paw	1491:1516	the arthritis affected paw	1491:1516	The developed MTX-MPs-H composite drug delivery system effectively decreased the swelling/ inflammation of the arthritis affected paw in wistar rats in comparison to only alginate microparticles and pure MTX up to 30 days.
33609936	7	41	from	nature	1376:1381	arg1	non-toxic					1363:1371	non-toxic	1363:1371	non-toxic	1363:1371	The in-vivo biocompatibility studies for 10 days revealed that composite system is non-toxic in nature.
33609936	6	42	theme	at	1187:1188	arg1	37 °C					1190:1194	gel at 37 °C	1183:1194	gel at 37 °C	1183:1194	Microparticles-hydrogel composite system exhibited appropriate in-vitro thermoreversibility (sol at 4 °C and gel at 37 °C), biocompatibility (>80 %), hemocompatibility, and controlled drug release profile.
33609936	2	43	theme	target	412:417	arg1	site					419:422	the target site	408:422	the target site in body	408:430	Nonetheless, owing to initial burst drug release, non-specific biodistribution and low retention time at the target site in body, these polymeric drug delivery systems have been found with low in-vivo performance.
33609936	3	44	theme	above	528:532	arg1	limitations					534:544	the above limitations	524:544	the above limitations	524:544	Hence, the above limitations need to be resolved by designing a smart novel drug delivery system which is the current need in biomedicine.
33609936	1	45	theme	arthritis	292:300	arg1	treatment					279:287	the treatment	275:287	the treatment of arthritis	275:300	Polymeric hydrogels and microparticles have been widely used for localized drug delivery applications for the treatment of arthritis.
33609936	2	46	theme	burst	333:337	arg1	release					344:350	initial burst drug release	325:350	initial burst drug release	325:350	Nonetheless, owing to initial burst drug release, non-specific biodistribution and low retention time at the target site in body, these polymeric drug delivery systems have been found with low in-vivo performance.
33609936	3	47	theme	novel	587:591	arg1	need					635:638	the current need	623:638	the current need in biomedicine	623:653	Hence, the above limitations need to be resolved by designing a smart novel drug delivery system which is the current need in biomedicine.
33609936	3	47	theme	novel	587:591	arg1	system					607:612	a smart novel drug delivery system	579:612	a smart novel drug delivery system which is the current need in biomedicine	579:653	Hence, the above limitations need to be resolved by designing a smart novel drug delivery system which is the current need in biomedicine.
33609936	8	48	theme	developed	1388:1396	arg1	system					1432:1437	The developed MTX-MPs-H composite drug delivery system	1384:1437	The developed MTX-MPs-H composite drug delivery system	1384:1437	The developed MTX-MPs-H composite drug delivery system effectively decreased the swelling/ inflammation of the arthritis affected paw in wistar rats in comparison to only alginate microparticles and pure MTX up to 30 days.
33609936	5	49	theme	alginate	886:893	arg1	microparticles					895:908	methotrexate (MTX) loaded alginate microparticles	860:908	methotrexate (MTX) loaded alginate microparticles (MTX-Microparticles)	860:929	In the current study, methotrexate (MTX) loaded alginate microparticles (MTX-Microparticles) are embedded into thermoreversible hydrogel matrix (MTX-MPs-H) prepared by physical blending of sodium hyaluronate and methylcellulose (SHMC).
33609936	5	49	theme	alginate	886:893	arg1	MTX-Microparticles					911:928	MTX-Microparticles	911:928	MTX-Microparticles	911:928	In the current study, methotrexate (MTX) loaded alginate microparticles (MTX-Microparticles) are embedded into thermoreversible hydrogel matrix (MTX-MPs-H) prepared by physical blending of sodium hyaluronate and methylcellulose (SHMC).
33609936	2	50	theme	non-specific	353:364	arg1	biodistribution					366:380	non-specific biodistribution	353:380	non-specific biodistribution	353:380	Nonetheless, owing to initial burst drug release, non-specific biodistribution and low retention time at the target site in body, these polymeric drug delivery systems have been found with low in-vivo performance.
33609936	4	51	theme	localized	672:680	arg1	system					767:772	a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system	664:772	a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system	664:772	Herein, a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system has been developed for the treatment of inflammatory arthritis.
33609936	1	52	theme	localized	234:242	arg1	applications					258:269	localized drug delivery applications	234:269	localized drug delivery applications for the treatment of arthritis	234:300	Polymeric hydrogels and microparticles have been widely used for localized drug delivery applications for the treatment of arthritis.
33609936	1	53	theme	delivery	249:256	arg1	applications					258:269	localized drug delivery applications	234:269	localized drug delivery applications for the treatment of arthritis	234:300	Polymeric hydrogels and microparticles have been widely used for localized drug delivery applications for the treatment of arthritis.
33609936	3	54	theme	delivery	598:605	arg1	need					635:638	the current need	623:638	the current need in biomedicine	623:653	Hence, the above limitations need to be resolved by designing a smart novel drug delivery system which is the current need in biomedicine.
33609936	3	54	theme	delivery	598:605	arg1	system					607:612	a smart novel drug delivery system	579:612	a smart novel drug delivery system which is the current need in biomedicine	579:653	Hence, the above limitations need to be resolved by designing a smart novel drug delivery system which is the current need in biomedicine.
33609936	5	55	theme	thermoreversible	949:964	arg1	MTX-MPs-H					983:991	MTX-MPs-H	983:991	MTX-MPs-H	983:991	In the current study, methotrexate (MTX) loaded alginate microparticles (MTX-Microparticles) are embedded into thermoreversible hydrogel matrix (MTX-MPs-H) prepared by physical blending of sodium hyaluronate and methylcellulose (SHMC).
33609936	5	55	theme	thermoreversible	949:964	arg1	matrix					975:980	thermoreversible hydrogel matrix	949:980	thermoreversible hydrogel matrix (MTX-MPs-H) prepared by physical blending of sodium hyaluronate and methylcellulose (SHMC)	949:1071	In the current study, methotrexate (MTX) loaded alginate microparticles (MTX-Microparticles) are embedded into thermoreversible hydrogel matrix (MTX-MPs-H) prepared by physical blending of sodium hyaluronate and methylcellulose (SHMC).
33609936	8	56	theme	only	1550:1553	arg1	microparticles					1564:1577	only alginate microparticles	1550:1577	only alginate microparticles	1550:1577	The developed MTX-MPs-H composite drug delivery system effectively decreased the swelling/ inflammation of the arthritis affected paw in wistar rats in comparison to only alginate microparticles and pure MTX up to 30 days.
33609936	4	57	theme	composite	743:751	arg1	system					767:772	a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system	664:772	a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system	664:772	Herein, a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system has been developed for the treatment of inflammatory arthritis.
33609936	6	58	theme	Microparticles-hydrogel	1074:1096	arg1	system					1108:1113	Microparticles-hydrogel composite system	1074:1113	Microparticles-hydrogel composite system	1074:1113	Microparticles-hydrogel composite system exhibited appropriate in-vitro thermoreversibility (sol at 4 °C and gel at 37 °C), biocompatibility (>80 %), hemocompatibility, and controlled drug release profile.
33609936	6	59	theme	drug	1258:1261	arg1	profile					1271:1277	controlled drug release profile	1247:1277	controlled drug release profile	1247:1277	Microparticles-hydrogel composite system exhibited appropriate in-vitro thermoreversibility (sol at 4 °C and gel at 37 °C), biocompatibility (>80 %), hemocompatibility, and controlled drug release profile.
33609936	4	60	theme	delivery	758:765	arg1	system					767:772	a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system	664:772	a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system	664:772	Herein, a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system has been developed for the treatment of inflammatory arthritis.
33609936	0	61	theme	composite	83:91	arg1	biomaterial					93:103	integrated hydrogel composite biomaterial	63:103	integrated hydrogel composite biomaterial: In-vivo biocompatibility and inflammatory arthritis treatment	63:166	Synthesis and characterization of an injectable microparticles integrated hydrogel composite biomaterial: In-vivo biocompatibility and inflammatory arthritis treatment.
33609936	8	62	theme	pure	1583:1586	arg1	MTX					1588:1590	pure MTX	1583:1590	pure MTX up to 30 days	1583:1604	The developed MTX-MPs-H composite drug delivery system effectively decreased the swelling/ inflammation of the arthritis affected paw in wistar rats in comparison to only alginate microparticles and pure MTX up to 30 days.
33609936	4	63	theme	thermoresponsive	693:708	arg1	system					767:772	a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system	664:772	a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system	664:772	Herein, a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system has been developed for the treatment of inflammatory arthritis.
33609936	4	64	theme	embedded	725:732	arg1	system					767:772	a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system	664:772	a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system	664:772	Herein, a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system has been developed for the treatment of inflammatory arthritis.
33609936	8	65	theme	arthritis	1495:1503	arg1	paw					1514:1516	the arthritis affected paw	1491:1516	the arthritis affected paw	1491:1516	The developed MTX-MPs-H composite drug delivery system effectively decreased the swelling/ inflammation of the arthritis affected paw in wistar rats in comparison to only alginate microparticles and pure MTX up to 30 days.
33609936	5	66	theme	physical	1006:1013	arg1	blending					1015:1022	physical blending	1006:1022	physical blending of sodium hyaluronate and methylcellulose (SHMC)	1006:1071	In the current study, methotrexate (MTX) loaded alginate microparticles (MTX-Microparticles) are embedded into thermoreversible hydrogel matrix (MTX-MPs-H) prepared by physical blending of sodium hyaluronate and methylcellulose (SHMC).
33609936	2	67	theme	drug	339:342	arg1	release					344:350	initial burst drug release	325:350	initial burst drug release	325:350	Nonetheless, owing to initial burst drug release, non-specific biodistribution and low retention time at the target site in body, these polymeric drug delivery systems have been found with low in-vivo performance.
33609936	8	68	theme	MTX-MPs-H	1398:1406	arg1	system					1432:1437	The developed MTX-MPs-H composite drug delivery system	1384:1437	The developed MTX-MPs-H composite drug delivery system	1384:1437	The developed MTX-MPs-H composite drug delivery system effectively decreased the swelling/ inflammation of the arthritis affected paw in wistar rats in comparison to only alginate microparticles and pure MTX up to 30 days.
33609936	0	69	theme	inflammatory	135:146	arg1	arthritis					148:156	inflammatory arthritis	135:156	inflammatory arthritis treatment	135:166	Synthesis and characterization of an injectable microparticles integrated hydrogel composite biomaterial: In-vivo biocompatibility and inflammatory arthritis treatment.
33609936	8	70	theme	drug	1418:1421	arg1	system					1432:1437	The developed MTX-MPs-H composite drug delivery system	1384:1437	The developed MTX-MPs-H composite drug delivery system	1384:1437	The developed MTX-MPs-H composite drug delivery system effectively decreased the swelling/ inflammation of the arthritis affected paw in wistar rats in comparison to only alginate microparticles and pure MTX up to 30 days.
33609936	3	71	from	need	635:638	arg1	biomedicine					643:653	biomedicine	643:653	biomedicine	643:653	Hence, the above limitations need to be resolved by designing a smart novel drug delivery system which is the current need in biomedicine.
33609936	5	72	theme	current	845:851	arg1	study					853:857	the current study	841:857	the current study	841:857	In the current study, methotrexate (MTX) loaded alginate microparticles (MTX-Microparticles) are embedded into thermoreversible hydrogel matrix (MTX-MPs-H) prepared by physical blending of sodium hyaluronate and methylcellulose (SHMC).
33609936	5	73	from	study	853:857	arg1	embedded					935:942	embedded	935:942	embedded	935:942	In the current study, methotrexate (MTX) loaded alginate microparticles (MTX-Microparticles) are embedded into thermoreversible hydrogel matrix (MTX-MPs-H) prepared by physical blending of sodium hyaluronate and methylcellulose (SHMC).
33609936	6	74	theme	appropriate	1125:1135	arg1	thermoreversibility					1146:1164	appropriate in-vitro thermoreversibility	1125:1164	appropriate in-vitro thermoreversibility (sol at 4 °C and gel at 37 °C)	1125:1195	Microparticles-hydrogel composite system exhibited appropriate in-vitro thermoreversibility (sol at 4 °C and gel at 37 °C), biocompatibility (>80 %), hemocompatibility, and controlled drug release profile.
33609936	4	75	theme	inflammatory	814:825	arg1	arthritis					827:835	inflammatory arthritis	814:835	inflammatory arthritis	814:835	Herein, a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system has been developed for the treatment of inflammatory arthritis.
33609936	2	76	theme	drug	449:452	arg1	systems					463:469	these polymeric drug delivery systems	433:469	these polymeric drug delivery systems	433:469	Nonetheless, owing to initial burst drug release, non-specific biodistribution and low retention time at the target site in body, these polymeric drug delivery systems have been found with low in-vivo performance.
33609936	0	77	theme	injectable	37:46	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of an injectable microparticles integrated hydrogel composite biomaterial: In-vivo biocompatibility and inflammatory arthritis treatment.
33609936	0	77	theme	injectable	37:46	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of an injectable microparticles integrated hydrogel composite biomaterial: In-vivo biocompatibility and inflammatory arthritis treatment.
33609936	2	78	theme	in-vivo	496:502	arg1	performance					504:514	low in-vivo performance	492:514	low in-vivo performance	492:514	Nonetheless, owing to initial burst drug release, non-specific biodistribution and low retention time at the target site in body, these polymeric drug delivery systems have been found with low in-vivo performance.
33609936	8	79	theme	swelling/	1465:1473	arg1	inflammation					1475:1486	the swelling/ inflammation	1461:1486	the swelling/ inflammation of the arthritis affected paw in wistar rats	1461:1531	The developed MTX-MPs-H composite drug delivery system effectively decreased the swelling/ inflammation of the arthritis affected paw in wistar rats in comparison to only alginate microparticles and pure MTX up to 30 days.
33609936	6	80	from	37 °C	1190:1194	arg1	gel					1183:1185	gel at 37 °C	1183:1194	gel at 37 °C	1183:1194	Microparticles-hydrogel composite system exhibited appropriate in-vitro thermoreversibility (sol at 4 °C and gel at 37 °C), biocompatibility (>80 %), hemocompatibility, and controlled drug release profile.
33609936	6	80	from	37 °C	1190:1194	arg1	at					1187:1188	gel at 37 °C	1183:1194	gel at 37 °C	1183:1194	Microparticles-hydrogel composite system exhibited appropriate in-vitro thermoreversibility (sol at 4 °C and gel at 37 °C), biocompatibility (>80 %), hemocompatibility, and controlled drug release profile.
33609936	6	80	from	37 °C	1190:1194	arg1	sol					1167:1169	sol	1167:1169	sol	1167:1169	Microparticles-hydrogel composite system exhibited appropriate in-vitro thermoreversibility (sol at 4 °C and gel at 37 °C), biocompatibility (>80 %), hemocompatibility, and controlled drug release profile.
33609936	0	81	theme	integrated	63:72	arg1	biomaterial					93:103	integrated hydrogel composite biomaterial	63:103	integrated hydrogel composite biomaterial: In-vivo biocompatibility and inflammatory arthritis treatment	63:166	Synthesis and characterization of an injectable microparticles integrated hydrogel composite biomaterial: In-vivo biocompatibility and inflammatory arthritis treatment.
33609936	2	82	from	site	419:422	arg1	body					427:430	body	427:430	body	427:430	Nonetheless, owing to initial burst drug release, non-specific biodistribution and low retention time at the target site in body, these polymeric drug delivery systems have been found with low in-vivo performance.
33609936	2	83	theme	low	386:388	arg1	time					400:403	low retention time	386:403	low retention time	386:403	Nonetheless, owing to initial burst drug release, non-specific biodistribution and low retention time at the target site in body, these polymeric drug delivery systems have been found with low in-vivo performance.
33609936	5	84	theme	hyaluronate	1034:1044	arg1	blending					1015:1022	physical blending	1006:1022	physical blending of sodium hyaluronate and methylcellulose (SHMC)	1006:1071	In the current study, methotrexate (MTX) loaded alginate microparticles (MTX-Microparticles) are embedded into thermoreversible hydrogel matrix (MTX-MPs-H) prepared by physical blending of sodium hyaluronate and methylcellulose (SHMC).
33609936	4	85	theme	arthritis	827:835	arg1	treatment					801:809	the treatment	797:809	the treatment of inflammatory arthritis	797:835	Herein, a novel localized injectable thermoresponsive microparticles embedded hydrogel composite drug delivery system has been developed for the treatment of inflammatory arthritis.
33609936	6	86	from	4 °C	1174:1177	arg1	sol					1167:1169	sol	1167:1169	sol	1167:1169	Microparticles-hydrogel composite system exhibited appropriate in-vitro thermoreversibility (sol at 4 °C and gel at 37 °C), biocompatibility (>80 %), hemocompatibility, and controlled drug release profile.
33609936	8	87	dep	days	1601:1604	arg1	up					1592:1593	up	1592:1593	up	1592:1593	The developed MTX-MPs-H composite drug delivery system effectively decreased the swelling/ inflammation of the arthritis affected paw in wistar rats in comparison to only alginate microparticles and pure MTX up to 30 days.
33609936	5	88	theme	methylcellulose	1050:1064	arg1	blending					1015:1022	physical blending	1006:1022	physical blending of sodium hyaluronate and methylcellulose (SHMC)	1006:1071	In the current study, methotrexate (MTX) loaded alginate microparticles (MTX-Microparticles) are embedded into thermoreversible hydrogel matrix (MTX-MPs-H) prepared by physical blending of sodium hyaluronate and methylcellulose (SHMC).
33609936	6	89	theme	gel	1183:1185	arg1	37 °C					1190:1194	gel at 37 °C	1183:1194	gel at 37 °C	1183:1194	Microparticles-hydrogel composite system exhibited appropriate in-vitro thermoreversibility (sol at 4 °C and gel at 37 °C), biocompatibility (>80 %), hemocompatibility, and controlled drug release profile.
33609936	8	90	theme	paw	1514:1516	arg1	inflammation					1475:1486	the swelling/ inflammation	1461:1486	the swelling/ inflammation of the arthritis affected paw in wistar rats	1461:1531	The developed MTX-MPs-H composite drug delivery system effectively decreased the swelling/ inflammation of the arthritis affected paw in wistar rats in comparison to only alginate microparticles and pure MTX up to 30 days.
34787328	4	0	theme	underlying	558:567	arg1	mechanism					569:577	the underlying mechanism	554:577	the underlying mechanism	554:577	It has been shown the potential of AAPs to improve diabetes as an effective adjuvant, but the underlying mechanism remains unclear.
34787328	5	1	theme	potential	639:647	arg1	mechanism					649:657	potential mechanism	639:657	potential mechanism	639:657	In this study, we explored the effects and potential mechanism of AAPs on type 2 diabetes (T2D) using a high-fat diet and streptozotocin (STZ) induced C57BL/6J mice.
34787328	13	2	theme	specific	1969:1976	arg1	mechanism					1989:1997	the specific underlying mechanism	1965:1997	the specific underlying mechanism that AAPs ameliorates T2D through regulating AKT/AMPK pathways and modifying the gut microbiota	1965:2093	Furthermore, our study firstly determined the specific underlying mechanism that AAPs ameliorates T2D through regulating AKT/AMPK pathways and modifying the gut microbiota.
34787328	8	3	theme	gut	1126:1128	arg1	microbiota					1130:1139	gut microbiota	1126:1139	gut microbiota	1126:1139	Furthermore, we investigated the association between changes of gut microbiota and AAPs effects using high-throughput sequencing of 16S rDNA for fecal samples.
34787328	12	4	theme	positive	1775:1782	arg1	factors					1891:1897	key factors	1887:1897	key factors closely related to T2D	1887:1920	PRACTICAL APPLICATION: The article systematically verified the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury, key factors closely related to T2D.
34787328	12	4	theme	positive	1775:1782	arg1	effects					1784:1790	the positive effects	1771:1790	the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury	1771:1884	PRACTICAL APPLICATION: The article systematically verified the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury, key factors closely related to T2D.
34787328	8	5	theme	rDNA	1198:1201	arg1	sequencing					1180:1189	high-throughput sequencing	1164:1189	high-throughput sequencing of 16S rDNA for fecal samples	1164:1219	Furthermore, we investigated the association between changes of gut microbiota and AAPs effects using high-throughput sequencing of 16S rDNA for fecal samples.
34787328	7	6	theme	T2D	1052:1054	arg1	mice					1056:1059	T2D mice	1052:1059	T2D mice	1052:1059	In addition, AAPs improved glycolipid metabolism disorders by activating the AKT and adenosine 5`monophosphate-activated protein kinase (AMPK) signaling pathways in T2D mice.
34787328	12	7	dep	APPLICATION	1722:1732	arg1	verified					1762:1769	verified	1762:1769	verified the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury, key factors closely related to T2D	1762:1920	PRACTICAL APPLICATION: The article systematically verified the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury, key factors closely related to T2D.
34787328	9	8	theme	decreased	1408:1416	arg1	abundance					1446:1454	decreased Clostridium and Allobaculum abundance	1408:1454	decreased Clostridium and Allobaculum abundance	1408:1454	In our study, AAPs elevated gut microbiota diversity and optimized microbial composition and function in T2D mice, characterized by increased Lactobacillus and Bacteroides abundance and decreased Clostridium and Allobaculum abundance.
34787328	9	9	from	diversity	1265:1273	arg1	mice					1331:1334	T2D mice	1327:1334	T2D mice	1327:1334	In our study, AAPs elevated gut microbiota diversity and optimized microbial composition and function in T2D mice, characterized by increased Lactobacillus and Bacteroides abundance and decreased Clostridium and Allobaculum abundance.
34787328	8	10	theme	fecal	1207:1211	arg1	samples					1213:1219	fecal samples	1207:1219	fecal samples	1207:1219	Furthermore, we investigated the association between changes of gut microbiota and AAPs effects using high-throughput sequencing of 16S rDNA for fecal samples.
34787328	14	11	theme	adjuvant	2172:2179	arg1	therapy					2181:2187	the adjuvant therapy	2168:2187	the adjuvant therapy of diabetes with AAPs	2168:2209	These results could offer a full explanation and a potential option for the adjuvant therapy of diabetes with AAPs.
34787328	2	12	theme	traditional	300:310	arg1	auricula-judae					181:194	Auricularia auricula-judae	169:194	Auricularia auricula-judae	169:194	Auricularia auricula-judae is an edible fungus with high nutritional value due to abundant polysaccharides, and is acknowledged as traditional food and medicine in Asia.
34787328	2	12	theme	traditional	300:310	arg1	food					312:315	traditional food	300:315	traditional food	300:315	Auricularia auricula-judae is an edible fungus with high nutritional value due to abundant polysaccharides, and is acknowledged as traditional food and medicine in Asia.
34787328	8	13	theme	high-throughput	1164:1178	arg1	sequencing					1180:1189	high-throughput sequencing	1164:1189	high-throughput sequencing of 16S rDNA for fecal samples	1164:1219	Furthermore, we investigated the association between changes of gut microbiota and AAPs effects using high-throughput sequencing of 16S rDNA for fecal samples.
34787328	3	14	from	auricula	363:370	arg1	polysaccharides					400:414	typically fungal polysaccharides	383:414	typically fungal polysaccharides	383:414	Polysaccharides from A. auricula (AAPs) are typically fungal polysaccharides and have a wide range of biological activities.
34787328	3	14	from	auricula	363:370	arg1	Polysaccharides					339:353	Polysaccharides	339:353	Polysaccharides from A. auricula (AAPs)	339:377	Polysaccharides from A. auricula (AAPs) are typically fungal polysaccharides and have a wide range of biological activities.
34787328	14	15	theme	potential	2147:2155	arg1	option					2157:2162	a potential option	2145:2162	a potential option for the adjuvant therapy of diabetes with AAPs	2145:2209	These results could offer a full explanation and a potential option for the adjuvant therapy of diabetes with AAPs.
34787328	7	16	theme	protein	1008:1014	arg1	kinase					1016:1021	the AKT and adenosine 5`monophosphate-activated protein kinase	960:1021	kinase	1016:1021	In addition, AAPs improved glycolipid metabolism disorders by activating the AKT and adenosine 5`monophosphate-activated protein kinase (AMPK) signaling pathways in T2D mice.
34787328	2	17	theme	high	221:224	arg1	value					238:242	high nutritional value	221:242	high nutritional value due to abundant polysaccharides	221:274	Auricularia auricula-judae is an edible fungus with high nutritional value due to abundant polysaccharides, and is acknowledged as traditional food and medicine in Asia.
34787328	1	18	theme	AKT/AMPK	117:124	arg1	pathways					136:143	the AKT/AMPK signaling pathways	113:143	the AKT/AMPK signaling pathways	113:143	polysaccharides improve type 2 diabetes in HFD/STZ-induced mice by regulating the AKT/AMPK signaling pathways and the gut microbiota.
34787328	7	19	theme	`	983:983	arg1	kinase					1016:1021	the AKT and adenosine 5`monophosphate-activated protein kinase	960:1021	kinase	1016:1021	In addition, AAPs improved glycolipid metabolism disorders by activating the AKT and adenosine 5`monophosphate-activated protein kinase (AMPK) signaling pathways in T2D mice.
34787328	13	20	theme	gut	2080:2082	arg1	microbiota					2084:2093	the gut microbiota	2076:2093	the gut microbiota	2076:2093	Furthermore, our study firstly determined the specific underlying mechanism that AAPs ameliorates T2D through regulating AKT/AMPK pathways and modifying the gut microbiota.
34787328	7	21	theme	metabolism	925:934	arg1	disorders					936:944	glycolipid metabolism disorders	914:944	glycolipid metabolism disorders	914:944	In addition, AAPs improved glycolipid metabolism disorders by activating the AKT and adenosine 5`monophosphate-activated protein kinase (AMPK) signaling pathways in T2D mice.
34787328	2	22	from	food	312:315	arg1	Asia					333:336	Asia	333:336	Asia	333:336	Auricularia auricula-judae is an edible fungus with high nutritional value due to abundant polysaccharides, and is acknowledged as traditional food and medicine in Asia.
34787328	2	23	from	medicine	321:328	arg1	Asia					333:336	Asia	333:336	Asia	333:336	Auricularia auricula-judae is an edible fungus with high nutritional value due to abundant polysaccharides, and is acknowledged as traditional food and medicine in Asia.
34787328	4	24	theme	AAPs	499:502	arg1	potential					486:494	the potential	482:494	the potential of AAPs to improve diabetes as an effective adjuvant	482:547	It has been shown the potential of AAPs to improve diabetes as an effective adjuvant, but the underlying mechanism remains unclear.
34787328	9	25	theme	gut	1250:1252	arg1	diversity					1265:1273	gut microbiota diversity	1250:1273	gut microbiota diversity	1250:1273	In our study, AAPs elevated gut microbiota diversity and optimized microbial composition and function in T2D mice, characterized by increased Lactobacillus and Bacteroides abundance and decreased Clostridium and Allobaculum abundance.
34787328	12	26	theme	key	1887:1889	arg1	factors					1891:1897	key factors	1887:1897	key factors closely related to T2D	1887:1920	PRACTICAL APPLICATION: The article systematically verified the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury, key factors closely related to T2D.
34787328	12	26	theme	key	1887:1889	arg1	effects					1784:1790	the positive effects	1771:1790	the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury	1771:1884	PRACTICAL APPLICATION: The article systematically verified the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury, key factors closely related to T2D.
34787328	0	27	theme	Auricularia	0:10	arg1	Bull					28:31	Bull	28:31	Bull	28:31	Auricularia auricula-judae (Bull.)
34787328	0	27	theme	Auricularia	0:10	arg1	auricula-judae					12:25	Auricularia auricula-judae	0:25	Auricularia auricula-judae (Bull.)	0:33	Auricularia auricula-judae (Bull.)
34787328	9	28	theme	optimized	1279:1287	arg1	composition					1299:1309	optimized microbial composition	1279:1309	optimized microbial composition	1279:1309	In our study, AAPs elevated gut microbiota diversity and optimized microbial composition and function in T2D mice, characterized by increased Lactobacillus and Bacteroides abundance and decreased Clostridium and Allobaculum abundance.
34787328	7	29	from	pathways	1040:1047	arg1	mice					1056:1059	T2D mice	1052:1059	T2D mice	1052:1059	In addition, AAPs improved glycolipid metabolism disorders by activating the AKT and adenosine 5`monophosphate-activated protein kinase (AMPK) signaling pathways in T2D mice.
34787328	13	30	dep	mechanism	1989:1997	arg1	ameliorates					2009:2019	ameliorates	2009:2019	ameliorates T2D through regulating AKT/AMPK pathways and modifying the gut microbiota	2009:2093	Furthermore, our study firstly determined the specific underlying mechanism that AAPs ameliorates T2D through regulating AKT/AMPK pathways and modifying the gut microbiota.
34787328	10	31	theme	AAPs	1471:1474	arg1	intervention					1476:1487	AAPs intervention	1471:1487	AAPs intervention	1471:1487	Particularly, AAPs intervention mainly affected the amino acid metabolism and glycolipid metabolism pathways.
34787328	11	32	theme	intestinal	1689:1698	arg1	microbiota					1700:1709	intestinal microbiota	1689:1709	intestinal microbiota	1689:1709	Overall, this study confirms that AAPs can improve type 2 diabetes by regulating the AKT and AMPK pathways and modulating intestinal microbiota.
34787328	3	33	theme	biological	441:450	arg1	activities					452:461	biological activities	441:461	biological activities	441:461	Polysaccharides from A. auricula (AAPs) are typically fungal polysaccharides and have a wide range of biological activities.
34787328	3	34	theme	fungal	393:398	arg1	polysaccharides					400:414	typically fungal polysaccharides	383:414	typically fungal polysaccharides	383:414	Polysaccharides from A. auricula (AAPs) are typically fungal polysaccharides and have a wide range of biological activities.
34787328	3	34	theme	fungal	393:398	arg1	Polysaccharides					339:353	Polysaccharides	339:353	Polysaccharides from A. auricula (AAPs)	339:377	Polysaccharides from A. auricula (AAPs) are typically fungal polysaccharides and have a wide range of biological activities.
34787328	10	35	theme	acid	1515:1518	arg1	metabolism					1520:1529	the amino acid metabolism	1505:1529	the amino acid metabolism	1505:1529	Particularly, AAPs intervention mainly affected the amino acid metabolism and glycolipid metabolism pathways.
34787328	5	36	theme	induced	739:745	arg1	mice					756:759	a high-fat diet and streptozotocin (STZ) induced C57BL/6J mice	698:759	a high-fat diet and streptozotocin (STZ) induced C57BL/6J mice	698:759	In this study, we explored the effects and potential mechanism of AAPs on type 2 diabetes (T2D) using a high-fat diet and streptozotocin (STZ) induced C57BL/6J mice.
34787328	9	37	theme	T2D	1327:1329	arg1	mice					1331:1334	T2D mice	1327:1334	T2D mice	1327:1334	In our study, AAPs elevated gut microbiota diversity and optimized microbial composition and function in T2D mice, characterized by increased Lactobacillus and Bacteroides abundance and decreased Clostridium and Allobaculum abundance.
34787328	12	38	theme	metabolism	1834:1843	arg1	disorder					1845:1852	glycolipid metabolism disorder	1823:1852	glycolipid metabolism disorder	1823:1852	PRACTICAL APPLICATION: The article systematically verified the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury, key factors closely related to T2D.
34787328	13	39	theme	AKT/AMPK	2044:2051	arg1	pathways					2053:2060	AKT/AMPK pathways	2044:2060	AKT/AMPK pathways	2044:2060	Furthermore, our study firstly determined the specific underlying mechanism that AAPs ameliorates T2D through regulating AKT/AMPK pathways and modifying the gut microbiota.
34787328	12	40	theme	AAPs	1795:1798	arg1	factors					1891:1897	key factors	1887:1897	key factors closely related to T2D	1887:1920	PRACTICAL APPLICATION: The article systematically verified the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury, key factors closely related to T2D.
34787328	12	40	theme	AAPs	1795:1798	arg1	effects					1784:1790	the positive effects	1771:1790	the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury	1771:1884	PRACTICAL APPLICATION: The article systematically verified the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury, key factors closely related to T2D.
34787328	10	41	theme	metabolism	1546:1555	arg1	pathways					1557:1564	glycolipid metabolism pathways	1535:1564	glycolipid metabolism pathways	1535:1564	Particularly, AAPs intervention mainly affected the amino acid metabolism and glycolipid metabolism pathways.
34787328	11	42	theme	type	1618:1621	arg1	diabetes					1625:1632	type 2 diabetes	1618:1632	type 2 diabetes	1618:1632	Overall, this study confirms that AAPs can improve type 2 diabetes by regulating the AKT and AMPK pathways and modulating intestinal microbiota.
34787328	5	43	from	mechanism	649:657	arg1	T2D					687:689	T2D	687:689	T2D	687:689	In this study, we explored the effects and potential mechanism of AAPs on type 2 diabetes (T2D) using a high-fat diet and streptozotocin (STZ) induced C57BL/6J mice.
34787328	5	43	from	mechanism	649:657	arg1	diabetes					677:684	type 2 diabetes	670:684	type 2 diabetes (T2D)	670:690	In this study, we explored the effects and potential mechanism of AAPs on type 2 diabetes (T2D) using a high-fat diet and streptozotocin (STZ) induced C57BL/6J mice.
34787328	6	44	theme	insulin	867:873	arg1	resistance					875:884	insulin resistance	867:884	insulin resistance	867:884	The results indicated that 50 and 100 mg/kg AAPs significantly decreased inflammation, liver injury, and insulin resistance.
34787328	12	45	theme	insulin	1803:1809	arg1	resistance					1811:1820	insulin resistance	1803:1820	insulin resistance	1803:1820	PRACTICAL APPLICATION: The article systematically verified the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury, key factors closely related to T2D.
34787328	9	46	theme	Lactobacillus	1364:1376	arg1	abundance					1394:1402	increased Lactobacillus and Bacteroides abundance	1354:1402	increased Lactobacillus and Bacteroides abundance	1354:1402	In our study, AAPs elevated gut microbiota diversity and optimized microbial composition and function in T2D mice, characterized by increased Lactobacillus and Bacteroides abundance and decreased Clostridium and Allobaculum abundance.
34787328	2	47	with	fungus	209:214	arg1	value					238:242	high nutritional value	221:242	high nutritional value due to abundant polysaccharides	221:274	Auricularia auricula-judae is an edible fungus with high nutritional value due to abundant polysaccharides, and is acknowledged as traditional food and medicine in Asia.
34787328	9	48	theme	Bacteroides	1382:1392	arg1	abundance					1394:1402	increased Lactobacillus and Bacteroides abundance	1354:1402	increased Lactobacillus and Bacteroides abundance	1354:1402	In our study, AAPs elevated gut microbiota diversity and optimized microbial composition and function in T2D mice, characterized by increased Lactobacillus and Bacteroides abundance and decreased Clostridium and Allobaculum abundance.
34787328	6	49	theme	liver	849:853	arg1	injury					855:860	liver injury	849:860	liver injury	849:860	The results indicated that 50 and 100 mg/kg AAPs significantly decreased inflammation, liver injury, and insulin resistance.
34787328	3	50	contain	have	420:423	arg1	polysaccharides					400:414	typically fungal polysaccharides	383:414	typically fungal polysaccharides	383:414	Polysaccharides from A. auricula (AAPs) are typically fungal polysaccharides and have a wide range of biological activities.
34787328	3	50	contain	have	420:423	arg1	Polysaccharides					339:353	Polysaccharides	339:353	Polysaccharides from A. auricula (AAPs)	339:377	Polysaccharides from A. auricula (AAPs) are typically fungal polysaccharides and have a wide range of biological activities.
34787328	3	50	contain	have	420:423	arg2	range					432:436	a wide range	425:436	a wide range of biological activities	425:461	Polysaccharides from A. auricula (AAPs) are typically fungal polysaccharides and have a wide range of biological activities.
34787328	11	51	theme	AMPK	1660:1663	arg1	pathways					1665:1672	the AKT and AMPK pathways	1648:1672	the AKT and AMPK pathways	1648:1672	Overall, this study confirms that AAPs can improve type 2 diabetes by regulating the AKT and AMPK pathways and modulating intestinal microbiota.
34787328	13	52	theme	underlying	1978:1987	arg1	mechanism					1989:1997	the specific underlying mechanism	1965:1997	the specific underlying mechanism that AAPs ameliorates T2D through regulating AKT/AMPK pathways and modifying the gut microbiota	1965:2093	Furthermore, our study firstly determined the specific underlying mechanism that AAPs ameliorates T2D through regulating AKT/AMPK pathways and modifying the gut microbiota.
34787328	5	53	dep	induced	739:745	arg1	diet					709:712	a high-fat diet	698:712	a high-fat diet	698:712	In this study, we explored the effects and potential mechanism of AAPs on type 2 diabetes (T2D) using a high-fat diet and streptozotocin (STZ) induced C57BL/6J mice.
34787328	5	53	dep	induced	739:745	arg1	STZ					734:736	STZ	734:736	STZ	734:736	In this study, we explored the effects and potential mechanism of AAPs on type 2 diabetes (T2D) using a high-fat diet and streptozotocin (STZ) induced C57BL/6J mice.
34787328	5	53	dep	induced	739:745	arg1	streptozotocin					718:731	streptozotocin	718:731	streptozotocin (STZ)	718:737	In this study, we explored the effects and potential mechanism of AAPs on type 2 diabetes (T2D) using a high-fat diet and streptozotocin (STZ) induced C57BL/6J mice.
34787328	8	54	theme	16S	1194:1196	arg1	rDNA					1198:1201	16S rDNA	1194:1201	16S rDNA	1194:1201	Furthermore, we investigated the association between changes of gut microbiota and AAPs effects using high-throughput sequencing of 16S rDNA for fecal samples.
34787328	14	55	theme	diabetes	2192:2199	arg1	therapy					2181:2187	the adjuvant therapy	2168:2187	the adjuvant therapy of diabetes with AAPs	2168:2209	These results could offer a full explanation and a potential option for the adjuvant therapy of diabetes with AAPs.
34787328	11	56	theme	AKT	1652:1654	arg1	pathways					1665:1672	the AKT and AMPK pathways	1648:1672	the AKT and AMPK pathways	1648:1672	Overall, this study confirms that AAPs can improve type 2 diabetes by regulating the AKT and AMPK pathways and modulating intestinal microbiota.
34787328	7	57	theme	signaling	1030:1038	arg1	pathways					1040:1047	the AKT and adenosine 5`monophosphate-activated protein kinase (AMPK) signaling pathways	960:1047	the AKT and adenosine 5`monophosphate-activated protein kinase (AMPK) signaling pathways in T2D mice	960:1059	In addition, AAPs improved glycolipid metabolism disorders by activating the AKT and adenosine 5`monophosphate-activated protein kinase (AMPK) signaling pathways in T2D mice.
34787328	9	58	theme	Clostridium	1418:1428	arg1	abundance					1446:1454	decreased Clostridium and Allobaculum abundance	1408:1454	decreased Clostridium and Allobaculum abundance	1408:1454	In our study, AAPs elevated gut microbiota diversity and optimized microbial composition and function in T2D mice, characterized by increased Lactobacillus and Bacteroides abundance and decreased Clostridium and Allobaculum abundance.
34787328	8	59	theme	microbiota	1130:1139	arg1	changes					1115:1121	changes	1115:1121	changes of gut microbiota and AAPs effects	1115:1156	Furthermore, we investigated the association between changes of gut microbiota and AAPs effects using high-throughput sequencing of 16S rDNA for fecal samples.
34787328	5	60	theme	type	670:673	arg1	T2D					687:689	T2D	687:689	T2D	687:689	In this study, we explored the effects and potential mechanism of AAPs on type 2 diabetes (T2D) using a high-fat diet and streptozotocin (STZ) induced C57BL/6J mice.
34787328	5	60	theme	type	670:673	arg1	diabetes					677:684	type 2 diabetes	670:684	type 2 diabetes (T2D)	670:690	In this study, we explored the effects and potential mechanism of AAPs on type 2 diabetes (T2D) using a high-fat diet and streptozotocin (STZ) induced C57BL/6J mice.
34787328	9	61	theme	Allobaculum	1434:1444	arg1	abundance					1446:1454	decreased Clostridium and Allobaculum abundance	1408:1454	decreased Clostridium and Allobaculum abundance	1408:1454	In our study, AAPs elevated gut microbiota diversity and optimized microbial composition and function in T2D mice, characterized by increased Lactobacillus and Bacteroides abundance and decreased Clostridium and Allobaculum abundance.
34787328	8	62	theme	AAPs	1145:1148	arg1	changes					1115:1121	changes	1115:1121	changes of gut microbiota and AAPs effects	1115:1156	Furthermore, we investigated the association between changes of gut microbiota and AAPs effects using high-throughput sequencing of 16S rDNA for fecal samples.
34787328	7	63	theme	kinase	1016:1021	arg1	pathways					1040:1047	the AKT and adenosine 5`monophosphate-activated protein kinase (AMPK) signaling pathways	960:1047	the AKT and adenosine 5`monophosphate-activated protein kinase (AMPK) signaling pathways in T2D mice	960:1059	In addition, AAPs improved glycolipid metabolism disorders by activating the AKT and adenosine 5`monophosphate-activated protein kinase (AMPK) signaling pathways in T2D mice.
34787328	2	64	theme	due	244:246	arg1	value					238:242	high nutritional value	221:242	high nutritional value due to abundant polysaccharides	221:274	Auricularia auricula-judae is an edible fungus with high nutritional value due to abundant polysaccharides, and is acknowledged as traditional food and medicine in Asia.
34787328	7	65	theme	monophosphate-activated	984:1006	arg1	kinase					1016:1021	the AKT and adenosine 5`monophosphate-activated protein kinase	960:1021	kinase	1016:1021	In addition, AAPs improved glycolipid metabolism disorders by activating the AKT and adenosine 5`monophosphate-activated protein kinase (AMPK) signaling pathways in T2D mice.
34787328	2	66	theme	nutritional	226:236	arg1	value					238:242	high nutritional value	221:242	high nutritional value due to abundant polysaccharides	221:274	Auricularia auricula-judae is an edible fungus with high nutritional value due to abundant polysaccharides, and is acknowledged as traditional food and medicine in Asia.
34787328	7	67	theme	adenosine	972:980	arg1	kinase					1016:1021	the AKT and adenosine 5`monophosphate-activated protein kinase	960:1021	kinase	1016:1021	In addition, AAPs improved glycolipid metabolism disorders by activating the AKT and adenosine 5`monophosphate-activated protein kinase (AMPK) signaling pathways in T2D mice.
34787328	5	68	theme	AAPs	662:665	arg1	effects					627:633	effects	627:633	effects	627:633	In this study, we explored the effects and potential mechanism of AAPs on type 2 diabetes (T2D) using a high-fat diet and streptozotocin (STZ) induced C57BL/6J mice.
34787328	5	68	theme	AAPs	662:665	arg1	mechanism					649:657	potential mechanism	639:657	potential mechanism	639:657	In this study, we explored the effects and potential mechanism of AAPs on type 2 diabetes (T2D) using a high-fat diet and streptozotocin (STZ) induced C57BL/6J mice.
34787328	7	69	theme	AKT	964:966	arg1	pathways					1040:1047	the AKT and adenosine 5`monophosphate-activated protein kinase (AMPK) signaling pathways	960:1047	the AKT and adenosine 5`monophosphate-activated protein kinase (AMPK) signaling pathways in T2D mice	960:1059	In addition, AAPs improved glycolipid metabolism disorders by activating the AKT and adenosine 5`monophosphate-activated protein kinase (AMPK) signaling pathways in T2D mice.
34787328	1	70	theme	signaling	126:134	arg1	pathways					136:143	the AKT/AMPK signaling pathways	113:143	the AKT/AMPK signaling pathways	113:143	polysaccharides improve type 2 diabetes in HFD/STZ-induced mice by regulating the AKT/AMPK signaling pathways and the gut microbiota.
34787328	5	71	theme	high-fat	700:707	arg1	diet					709:712	a high-fat diet	698:712	a high-fat diet	698:712	In this study, we explored the effects and potential mechanism of AAPs on type 2 diabetes (T2D) using a high-fat diet and streptozotocin (STZ) induced C57BL/6J mice.
34787328	2	72	theme	abundant	251:258	arg1	polysaccharides					260:274	abundant polysaccharides	251:274	abundant polysaccharides	251:274	Auricularia auricula-judae is an edible fungus with high nutritional value due to abundant polysaccharides, and is acknowledged as traditional food and medicine in Asia.
34787328	12	73	theme	PRACTICAL	1712:1720	arg1	APPLICATION					1722:1732	PRACTICAL APPLICATION	1712:1732	PRACTICAL APPLICATION: The article systematically verified the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury, key factors closely related to T2D.	1712:1921	PRACTICAL APPLICATION: The article systematically verified the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury, key factors closely related to T2D.
34787328	1	74	theme	gut	153:155	arg1	microbiota					157:166	the gut microbiota	149:166	the gut microbiota	149:166	polysaccharides improve type 2 diabetes in HFD/STZ-induced mice by regulating the AKT/AMPK signaling pathways and the gut microbiota.
34787328	9	75	from	function	1315:1322	arg1	mice					1331:1334	T2D mice	1327:1334	T2D mice	1327:1334	In our study, AAPs elevated gut microbiota diversity and optimized microbial composition and function in T2D mice, characterized by increased Lactobacillus and Bacteroides abundance and decreased Clostridium and Allobaculum abundance.
34787328	7	76	theme	glycolipid	914:923	arg1	disorders					936:944	glycolipid metabolism disorders	914:944	glycolipid metabolism disorders	914:944	In addition, AAPs improved glycolipid metabolism disorders by activating the AKT and adenosine 5`monophosphate-activated protein kinase (AMPK) signaling pathways in T2D mice.
34787328	1	77	theme	type	59:62	arg1	diabetes					66:73	type 2 diabetes	59:73	type 2 diabetes	59:73	polysaccharides improve type 2 diabetes in HFD/STZ-induced mice by regulating the AKT/AMPK signaling pathways and the gut microbiota.
34787328	14	78	theme	full	2124:2127	arg1	explanation					2129:2139	a full explanation	2122:2139	a full explanation	2122:2139	These results could offer a full explanation and a potential option for the adjuvant therapy of diabetes with AAPs.
34787328	9	79	theme	microbiota	1254:1263	arg1	diversity					1265:1273	gut microbiota diversity	1250:1273	gut microbiota diversity	1250:1273	In our study, AAPs elevated gut microbiota diversity and optimized microbial composition and function in T2D mice, characterized by increased Lactobacillus and Bacteroides abundance and decreased Clostridium and Allobaculum abundance.
34787328	9	80	from	composition	1299:1309	arg1	mice					1331:1334	T2D mice	1327:1334	T2D mice	1327:1334	In our study, AAPs elevated gut microbiota diversity and optimized microbial composition and function in T2D mice, characterized by increased Lactobacillus and Bacteroides abundance and decreased Clostridium and Allobaculum abundance.
34787328	12	81	from	effects	1784:1790	arg1	inflammation					1855:1866	inflammation	1855:1866	inflammation	1855:1866	PRACTICAL APPLICATION: The article systematically verified the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury, key factors closely related to T2D.
34787328	12	81	from	effects	1784:1790	arg1	disorder					1845:1852	glycolipid metabolism disorder	1823:1852	glycolipid metabolism disorder	1823:1852	PRACTICAL APPLICATION: The article systematically verified the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury, key factors closely related to T2D.
34787328	12	81	from	effects	1784:1790	arg1	injury					1879:1884	liver injury	1873:1884	liver injury	1873:1884	PRACTICAL APPLICATION: The article systematically verified the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury, key factors closely related to T2D.
34787328	12	81	from	effects	1784:1790	arg1	resistance					1811:1820	insulin resistance	1803:1820	insulin resistance	1803:1820	PRACTICAL APPLICATION: The article systematically verified the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury, key factors closely related to T2D.
34787328	14	82	with	diabetes	2192:2199	arg1	AAPs					2206:2209	AAPs	2206:2209	AAPs	2206:2209	These results could offer a full explanation and a potential option for the adjuvant therapy of diabetes with AAPs.
34787328	5	83	theme	C57BL/6J	747:754	arg1	mice					756:759	a high-fat diet and streptozotocin (STZ) induced C57BL/6J mice	698:759	a high-fat diet and streptozotocin (STZ) induced C57BL/6J mice	698:759	In this study, we explored the effects and potential mechanism of AAPs on type 2 diabetes (T2D) using a high-fat diet and streptozotocin (STZ) induced C57BL/6J mice.
34787328	12	84	theme	related	1907:1913	arg1	factors					1891:1897	key factors	1887:1897	key factors closely related to T2D	1887:1920	PRACTICAL APPLICATION: The article systematically verified the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury, key factors closely related to T2D.
34787328	12	84	theme	related	1907:1913	arg1	effects					1784:1790	the positive effects	1771:1790	the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury	1771:1884	PRACTICAL APPLICATION: The article systematically verified the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury, key factors closely related to T2D.
34787328	1	85	theme	HFD/STZ-induced	78:92	arg1	mice					94:97	HFD/STZ-induced mice	78:97	HFD/STZ-induced mice	78:97	polysaccharides improve type 2 diabetes in HFD/STZ-induced mice by regulating the AKT/AMPK signaling pathways and the gut microbiota.
34787328	3	86	theme	wide	427:430	arg1	range					432:436	a wide range	425:436	a wide range of biological activities	425:461	Polysaccharides from A. auricula (AAPs) are typically fungal polysaccharides and have a wide range of biological activities.
34787328	9	87	theme	microbial	1289:1297	arg1	composition					1299:1309	optimized microbial composition	1279:1309	optimized microbial composition	1279:1309	In our study, AAPs elevated gut microbiota diversity and optimized microbial composition and function in T2D mice, characterized by increased Lactobacillus and Bacteroides abundance and decreased Clostridium and Allobaculum abundance.
34787328	12	88	theme	liver	1873:1877	arg1	injury					1879:1884	liver injury	1873:1884	liver injury	1873:1884	PRACTICAL APPLICATION: The article systematically verified the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury, key factors closely related to T2D.
34787328	5	89	dep	effects	627:633	arg1	the					623:625	the	623:625	the	623:625	In this study, we explored the effects and potential mechanism of AAPs on type 2 diabetes (T2D) using a high-fat diet and streptozotocin (STZ) induced C57BL/6J mice.
34787328	2	90	theme	Auricularia	169:179	arg1	auricula-judae					181:194	Auricularia auricula-judae	169:194	Auricularia auricula-judae	169:194	Auricularia auricula-judae is an edible fungus with high nutritional value due to abundant polysaccharides, and is acknowledged as traditional food and medicine in Asia.
34787328	2	90	theme	Auricularia	169:179	arg1	food					312:315	traditional food	300:315	traditional food	300:315	Auricularia auricula-judae is an edible fungus with high nutritional value due to abundant polysaccharides, and is acknowledged as traditional food and medicine in Asia.
34787328	2	90	theme	Auricularia	169:179	arg1	fungus					209:214	an edible fungus	199:214	an edible fungus with high nutritional value due to abundant polysaccharides	199:274	Auricularia auricula-judae is an edible fungus with high nutritional value due to abundant polysaccharides, and is acknowledged as traditional food and medicine in Asia.
34787328	2	90	theme	Auricularia	169:179	arg1	medicine					321:328	medicine	321:328	medicine	321:328	Auricularia auricula-judae is an edible fungus with high nutritional value due to abundant polysaccharides, and is acknowledged as traditional food and medicine in Asia.
34787328	3	91	theme	activities	452:461	arg1	range					432:436	a wide range	425:436	a wide range of biological activities	425:461	Polysaccharides from A. auricula (AAPs) are typically fungal polysaccharides and have a wide range of biological activities.
34787328	10	92	theme	amino	1509:1513	arg1	metabolism					1520:1529	the amino acid metabolism	1505:1529	the amino acid metabolism	1505:1529	Particularly, AAPs intervention mainly affected the amino acid metabolism and glycolipid metabolism pathways.
34787328	5	93	from	effects	627:633	arg1	T2D					687:689	T2D	687:689	T2D	687:689	In this study, we explored the effects and potential mechanism of AAPs on type 2 diabetes (T2D) using a high-fat diet and streptozotocin (STZ) induced C57BL/6J mice.
34787328	5	93	from	effects	627:633	arg1	diabetes					677:684	type 2 diabetes	670:684	type 2 diabetes (T2D)	670:690	In this study, we explored the effects and potential mechanism of AAPs on type 2 diabetes (T2D) using a high-fat diet and streptozotocin (STZ) induced C57BL/6J mice.
34787328	12	94	theme	glycolipid	1823:1832	arg1	disorder					1845:1852	glycolipid metabolism disorder	1823:1852	glycolipid metabolism disorder	1823:1852	PRACTICAL APPLICATION: The article systematically verified the positive effects of AAPs on insulin resistance, glycolipid metabolism disorder, inflammation, and liver injury, key factors closely related to T2D.
34787328	8	95	dep	microbiota	1130:1139	arg1	effects					1150:1156	effects	1150:1156	effects	1150:1156	Furthermore, we investigated the association between changes of gut microbiota and AAPs effects using high-throughput sequencing of 16S rDNA for fecal samples.
34787328	2	96	theme	edible	202:207	arg1	auricula-judae					181:194	Auricularia auricula-judae	169:194	Auricularia auricula-judae	169:194	Auricularia auricula-judae is an edible fungus with high nutritional value due to abundant polysaccharides, and is acknowledged as traditional food and medicine in Asia.
34787328	2	96	theme	edible	202:207	arg1	fungus					209:214	an edible fungus	199:214	an edible fungus with high nutritional value due to abundant polysaccharides	199:274	Auricularia auricula-judae is an edible fungus with high nutritional value due to abundant polysaccharides, and is acknowledged as traditional food and medicine in Asia.
34787328	10	97	theme	glycolipid	1535:1544	arg1	pathways					1557:1564	glycolipid metabolism pathways	1535:1564	glycolipid metabolism pathways	1535:1564	Particularly, AAPs intervention mainly affected the amino acid metabolism and glycolipid metabolism pathways.
34787328	9	98	theme	increased	1354:1362	arg1	abundance					1394:1402	increased Lactobacillus and Bacteroides abundance	1354:1402	increased Lactobacillus and Bacteroides abundance	1354:1402	In our study, AAPs elevated gut microbiota diversity and optimized microbial composition and function in T2D mice, characterized by increased Lactobacillus and Bacteroides abundance and decreased Clostridium and Allobaculum abundance.
32942073	5	0	from	composition	965:975	arg1	behaviors					1064:1072	pharmacological behaviors	1048:1072	pharmacological behaviors	1048:1072	Given the prominent pharmacological properties, well-defined chemical composition and explicable relationships between dHG-5 and its oligosaccharides in pharmacological behaviors, dHG-5 is expected to be an ideal novel anticoagulant medicine.
32942073	4	1	theme	dHG-5	798:802	arg1	potencies					785:793	these activity potencies	770:793	these activity potencies of dHG-5	770:802	The relationships between anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y) and molecular weight (x) could be approximately expressed as the power function (y = a × xb), and these activity potencies of dHG-5 were approximately equivalent to the weighted average sum of that of its oligosaccharides.
32942073	1	2	theme	enzyme	132:137	arg1	Xase					91:94	Intrinsic Xase	81:94	Intrinsic Xase (iXase)	81:102	Intrinsic Xase (iXase), the last and rate-limiting enzyme complex in the intrinsic coagulation pathway, may be an ideal target for antithrombotic treatment.
32942073	1	2	theme	enzyme	132:137	arg1	complex					139:145	the last and rate-limiting enzyme complex	105:145	the last and rate-limiting enzyme complex in the intrinsic coagulation pathway	105:182	Intrinsic Xase (iXase), the last and rate-limiting enzyme complex in the intrinsic coagulation pathway, may be an ideal target for antithrombotic treatment.
32942073	1	3	theme	antithrombotic	212:225	arg1	treatment					227:235	antithrombotic treatment	212:235	antithrombotic treatment	212:235	Intrinsic Xase (iXase), the last and rate-limiting enzyme complex in the intrinsic coagulation pathway, may be an ideal target for antithrombotic treatment.
32942073	0	4	theme	dHG-5	74:78	arg1	analysis					30:37	analysis	30:37	The components and activities analysis of a novel anticoagulant candidate dHG-5.	0:79	The components and activities analysis of a novel anticoagulant candidate dHG-5.
32942073	3	5	theme	oligosaccharides	451:466	arg1	series					441:446	the series	437:446	the series of oligosaccharides contained in dHG-5	437:485	In this work, the series of oligosaccharides contained in dHG-5 were purified and their precise structures were confirmed by 2D NMR and MS spectra.
32942073	2	6	from	cucumber	305:312	arg1	fraction					254:261	A depolymerized fraction	238:261	A depolymerized fraction of fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata, dHG-5 (Mw 5.2 kDa),	238:358	A depolymerized fraction of fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata, dHG-5 (Mw 5.2 kDa), showed potent and selective inhibition of iXase (IC50, 14 nM).
32942073	5	7	theme	ideal	1102:1106	arg1	medicine					1128:1135	an ideal novel anticoagulant medicine	1099:1135	an ideal novel anticoagulant medicine	1099:1135	Given the prominent pharmacological properties, well-defined chemical composition and explicable relationships between dHG-5 and its oligosaccharides in pharmacological behaviors, dHG-5 is expected to be an ideal novel anticoagulant medicine.
32942073	5	7	theme	ideal	1102:1106	arg1	dHG-5					1075:1079	dHG-5	1075:1079	dHG-5	1075:1079	Given the prominent pharmacological properties, well-defined chemical composition and explicable relationships between dHG-5 and its oligosaccharides in pharmacological behaviors, dHG-5 is expected to be an ideal novel anticoagulant medicine.
32942073	1	8	from	complex	139:145	arg1	pathway					176:182	the intrinsic coagulation pathway	150:182	the intrinsic coagulation pathway	150:182	Intrinsic Xase (iXase), the last and rate-limiting enzyme complex in the intrinsic coagulation pathway, may be an ideal target for antithrombotic treatment.
32942073	2	9	theme	fucosylated	266:276	arg1	glycosaminoglycan					278:294	fucosylated glycosaminoglycan	266:294	fucosylated glycosaminoglycan	266:294	A depolymerized fraction of fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata, dHG-5 (Mw 5.2 kDa), showed potent and selective inhibition of iXase (IC50, 14 nM).
32942073	3	10	theme	precise	511:517	arg1	structures					519:528	their precise structures	505:528	their precise structures	505:528	In this work, the series of oligosaccharides contained in dHG-5 were purified and their precise structures were confirmed by 2D NMR and MS spectra.
32942073	2	11	gly	fucosylated	266:276	arg1	glycosaminoglycan					278:294	fucosylated glycosaminoglycan	266:294	fucosylated glycosaminoglycan	266:294	A depolymerized fraction of fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata, dHG-5 (Mw 5.2 kDa), showed potent and selective inhibition of iXase (IC50, 14 nM).
32942073	2	12	theme	Mw	347:348	arg1	dHG-5					340:344	dHG-5	340:344	dHG-5 (Mw 5.2 kDa)	340:357	A depolymerized fraction of fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata, dHG-5 (Mw 5.2 kDa), showed potent and selective inhibition of iXase (IC50, 14 nM).
32942073	2	12	theme	Mw	347:348	arg1	5.2 kDa					350:356	Mw 5.2 kDa	347:356	Mw 5.2 kDa	347:356	A depolymerized fraction of fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata, dHG-5 (Mw 5.2 kDa), showed potent and selective inhibition of iXase (IC50, 14 nM).
32942073	2	13	theme	Holothuria	314:323	arg1	dHG-5					340:344	dHG-5	340:344	dHG-5 (Mw 5.2 kDa)	340:357	A depolymerized fraction of fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata, dHG-5 (Mw 5.2 kDa), showed potent and selective inhibition of iXase (IC50, 14 nM).
32942073	2	13	theme	Holothuria	314:323	arg1	cucumber					305:312	sea cucumber Holothuria fuscopunctata	301:337	sea cucumber Holothuria fuscopunctata	301:337	A depolymerized fraction of fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata, dHG-5 (Mw 5.2 kDa), showed potent and selective inhibition of iXase (IC50, 14 nM).
32942073	5	14	theme	prominent	905:913	arg1	properties					931:940	the prominent pharmacological properties	901:940	the prominent pharmacological properties	901:940	Given the prominent pharmacological properties, well-defined chemical composition and explicable relationships between dHG-5 and its oligosaccharides in pharmacological behaviors, dHG-5 is expected to be an ideal novel anticoagulant medicine.
32942073	4	15	theme	anticoagulant	624:636	arg1	activities					657:666	anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities	597:666	anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y)	597:670	The relationships between anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y) and molecular weight (x) could be approximately expressed as the power function (y = a × xb), and these activity potencies of dHG-5 were approximately equivalent to the weighted average sum of that of its oligosaccharides.
32942073	4	15	theme	anticoagulant	624:636	arg1	y					669:669	y	669:669	y	669:669	The relationships between anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y) and molecular weight (x) could be approximately expressed as the power function (y = a × xb), and these activity potencies of dHG-5 were approximately equivalent to the weighted average sum of that of its oligosaccharides.
32942073	4	16	theme	f.IXa-binding	609:621	arg1	activities					657:666	anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities	597:666	anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y)	597:670	The relationships between anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y) and molecular weight (x) could be approximately expressed as the power function (y = a × xb), and these activity potencies of dHG-5 were approximately equivalent to the weighted average sum of that of its oligosaccharides.
32942073	4	16	theme	f.IXa-binding	609:621	arg1	y					669:669	y	669:669	y	669:669	The relationships between anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y) and molecular weight (x) could be approximately expressed as the power function (y = a × xb), and these activity potencies of dHG-5 were approximately equivalent to the weighted average sum of that of its oligosaccharides.
32942073	2	17	theme	depolymerized	240:252	arg1	fraction					254:261	A depolymerized fraction	238:261	A depolymerized fraction of fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata, dHG-5 (Mw 5.2 kDa),	238:358	A depolymerized fraction of fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata, dHG-5 (Mw 5.2 kDa), showed potent and selective inhibition of iXase (IC50, 14 nM).
32942073	5	18	from	properties	931:940	arg1	behaviors					1064:1072	pharmacological behaviors	1048:1072	pharmacological behaviors	1048:1072	Given the prominent pharmacological properties, well-defined chemical composition and explicable relationships between dHG-5 and its oligosaccharides in pharmacological behaviors, dHG-5 is expected to be an ideal novel anticoagulant medicine.
32942073	2	19	theme	potent	367:372	arg1	inhibition					388:397	potent and selective inhibition	367:397	potent and selective inhibition of iXase (IC50, 14 nM)	367:420	A depolymerized fraction of fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata, dHG-5 (Mw 5.2 kDa), showed potent and selective inhibition of iXase (IC50, 14 nM).
32942073	1	20	theme	Intrinsic	81:89	arg1	target					201:206	an ideal target	192:206	an ideal target for antithrombotic treatment	192:235	Intrinsic Xase (iXase), the last and rate-limiting enzyme complex in the intrinsic coagulation pathway, may be an ideal target for antithrombotic treatment.
32942073	1	20	theme	Intrinsic	81:89	arg1	Xase					91:94	Intrinsic Xase	81:94	Intrinsic Xase (iXase)	81:102	Intrinsic Xase (iXase), the last and rate-limiting enzyme complex in the intrinsic coagulation pathway, may be an ideal target for antithrombotic treatment.
32942073	1	20	theme	Intrinsic	81:89	arg1	complex					139:145	the last and rate-limiting enzyme complex	105:145	the last and rate-limiting enzyme complex in the intrinsic coagulation pathway	105:182	Intrinsic Xase (iXase), the last and rate-limiting enzyme complex in the intrinsic coagulation pathway, may be an ideal target for antithrombotic treatment.
32942073	1	20	theme	Intrinsic	81:89	arg1	iXase					97:101	iXase	97:101	iXase	97:101	Intrinsic Xase (iXase), the last and rate-limiting enzyme complex in the intrinsic coagulation pathway, may be an ideal target for antithrombotic treatment.
32942073	1	21	theme	intrinsic	154:162	arg1	pathway					176:182	the intrinsic coagulation pathway	150:182	the intrinsic coagulation pathway	150:182	Intrinsic Xase (iXase), the last and rate-limiting enzyme complex in the intrinsic coagulation pathway, may be an ideal target for antithrombotic treatment.
32942073	4	22	theme	activity	776:783	arg1	potencies					785:793	these activity potencies	770:793	these activity potencies of dHG-5	770:802	The relationships between anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y) and molecular weight (x) could be approximately expressed as the power function (y = a × xb), and these activity potencies of dHG-5 were approximately equivalent to the weighted average sum of that of its oligosaccharides.
32942073	2	23	theme	sea	301:303	arg1	dHG-5					340:344	dHG-5	340:344	dHG-5 (Mw 5.2 kDa)	340:357	A depolymerized fraction of fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata, dHG-5 (Mw 5.2 kDa), showed potent and selective inhibition of iXase (IC50, 14 nM).
32942073	2	23	theme	sea	301:303	arg1	cucumber					305:312	sea cucumber Holothuria fuscopunctata	301:337	sea cucumber Holothuria fuscopunctata	301:337	A depolymerized fraction of fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata, dHG-5 (Mw 5.2 kDa), showed potent and selective inhibition of iXase (IC50, 14 nM).
32942073	1	24	theme	coagulation	164:174	arg1	pathway					176:182	the intrinsic coagulation pathway	150:182	the intrinsic coagulation pathway	150:182	Intrinsic Xase (iXase), the last and rate-limiting enzyme complex in the intrinsic coagulation pathway, may be an ideal target for antithrombotic treatment.
32942073	5	25	theme	well-defined	943:954	arg1	composition					965:975	well-defined chemical composition	943:975	well-defined chemical composition	943:975	Given the prominent pharmacological properties, well-defined chemical composition and explicable relationships between dHG-5 and its oligosaccharides in pharmacological behaviors, dHG-5 is expected to be an ideal novel anticoagulant medicine.
32942073	5	26	from	relationships	992:1004	arg1	behaviors					1064:1072	pharmacological behaviors	1048:1072	pharmacological behaviors	1048:1072	Given the prominent pharmacological properties, well-defined chemical composition and explicable relationships between dHG-5 and its oligosaccharides in pharmacological behaviors, dHG-5 is expected to be an ideal novel anticoagulant medicine.
32942073	2	27	theme	iXase	402:406	arg1	inhibition					388:397	potent and selective inhibition	367:397	potent and selective inhibition of iXase (IC50, 14 nM)	367:420	A depolymerized fraction of fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata, dHG-5 (Mw 5.2 kDa), showed potent and selective inhibition of iXase (IC50, 14 nM).
32942073	4	28	theme	average	850:856	arg1	sum					858:860	the weighted average sum	837:860	the weighted average sum of that of its oligosaccharides	837:892	The relationships between anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y) and molecular weight (x) could be approximately expressed as the power function (y = a × xb), and these activity potencies of dHG-5 were approximately equivalent to the weighted average sum of that of its oligosaccharides.
32942073	2	29	theme	glycosaminoglycan	278:294	arg1	fraction					254:261	A depolymerized fraction	238:261	A depolymerized fraction of fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata, dHG-5 (Mw 5.2 kDa),	238:358	A depolymerized fraction of fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata, dHG-5 (Mw 5.2 kDa), showed potent and selective inhibition of iXase (IC50, 14 nM).
32942073	5	30	theme	explicable	981:990	arg1	relationships					992:1004	explicable relationships	981:1004	explicable relationships between dHG-5 and its oligosaccharides	981:1043	Given the prominent pharmacological properties, well-defined chemical composition and explicable relationships between dHG-5 and its oligosaccharides in pharmacological behaviors, dHG-5 is expected to be an ideal novel anticoagulant medicine.
32942073	5	31	theme	chemical	956:963	arg1	composition					965:975	well-defined chemical composition	943:975	well-defined chemical composition	943:975	Given the prominent pharmacological properties, well-defined chemical composition and explicable relationships between dHG-5 and its oligosaccharides in pharmacological behaviors, dHG-5 is expected to be an ideal novel anticoagulant medicine.
32942073	4	32	theme	that	865:868	arg1	sum					858:860	the weighted average sum	837:860	the weighted average sum of that of its oligosaccharides	837:892	The relationships between anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y) and molecular weight (x) could be approximately expressed as the power function (y = a × xb), and these activity potencies of dHG-5 were approximately equivalent to the weighted average sum of that of its oligosaccharides.
32942073	4	33	theme	anti-iXase	597:606	arg1	activities					657:666	anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities	597:666	anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y)	597:670	The relationships between anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y) and molecular weight (x) could be approximately expressed as the power function (y = a × xb), and these activity potencies of dHG-5 were approximately equivalent to the weighted average sum of that of its oligosaccharides.
32942073	4	33	theme	anti-iXase	597:606	arg1	y					669:669	y	669:669	y	669:669	The relationships between anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y) and molecular weight (x) could be approximately expressed as the power function (y = a × xb), and these activity potencies of dHG-5 were approximately equivalent to the weighted average sum of that of its oligosaccharides.
32942073	5	34	theme	novel	1108:1112	arg1	medicine					1128:1135	an ideal novel anticoagulant medicine	1099:1135	an ideal novel anticoagulant medicine	1099:1135	Given the prominent pharmacological properties, well-defined chemical composition and explicable relationships between dHG-5 and its oligosaccharides in pharmacological behaviors, dHG-5 is expected to be an ideal novel anticoagulant medicine.
32942073	5	34	theme	novel	1108:1112	arg1	dHG-5					1075:1079	dHG-5	1075:1079	dHG-5	1075:1079	Given the prominent pharmacological properties, well-defined chemical composition and explicable relationships between dHG-5 and its oligosaccharides in pharmacological behaviors, dHG-5 is expected to be an ideal novel anticoagulant medicine.
32942073	5	35	theme	pharmacological	1048:1062	arg1	behaviors					1064:1072	pharmacological behaviors	1048:1072	pharmacological behaviors	1048:1072	Given the prominent pharmacological properties, well-defined chemical composition and explicable relationships between dHG-5 and its oligosaccharides in pharmacological behaviors, dHG-5 is expected to be an ideal novel anticoagulant medicine.
32942073	3	36	theme	NMR	551:553	arg1	spectra					562:568	NMR and MS spectra	551:568	NMR and MS spectra	551:568	In this work, the series of oligosaccharides contained in dHG-5 were purified and their precise structures were confirmed by 2D NMR and MS spectra.
32942073	2	37	theme	selective	378:386	arg1	inhibition					388:397	potent and selective inhibition	367:397	potent and selective inhibition of iXase (IC50, 14 nM)	367:420	A depolymerized fraction of fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata, dHG-5 (Mw 5.2 kDa), showed potent and selective inhibition of iXase (IC50, 14 nM).
32942073	0	38	theme	novel	44:48	arg1	dHG-5					74:78	a novel anticoagulant candidate dHG-5	42:78	a novel anticoagulant candidate dHG-5	42:78	The components and activities analysis of a novel anticoagulant candidate dHG-5.
32942073	5	39	theme	anticoagulant	1114:1126	arg1	medicine					1128:1135	an ideal novel anticoagulant medicine	1099:1135	an ideal novel anticoagulant medicine	1099:1135	Given the prominent pharmacological properties, well-defined chemical composition and explicable relationships between dHG-5 and its oligosaccharides in pharmacological behaviors, dHG-5 is expected to be an ideal novel anticoagulant medicine.
32942073	5	39	theme	anticoagulant	1114:1126	arg1	dHG-5					1075:1079	dHG-5	1075:1079	dHG-5	1075:1079	Given the prominent pharmacological properties, well-defined chemical composition and explicable relationships between dHG-5 and its oligosaccharides in pharmacological behaviors, dHG-5 is expected to be an ideal novel anticoagulant medicine.
32942073	2	40	dep	iXase	402:406	arg1	14 nM					415:419	14 nM	415:419	14 nM	415:419	A depolymerized fraction of fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata, dHG-5 (Mw 5.2 kDa), showed potent and selective inhibition of iXase (IC50, 14 nM).
32942073	2	40	dep	iXase	402:406	arg1	IC50					409:412	IC50	409:412	IC50	409:412	A depolymerized fraction of fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata, dHG-5 (Mw 5.2 kDa), showed potent and selective inhibition of iXase (IC50, 14 nM).
32942073	4	41	theme	molecular	676:684	arg1	x					694:694	x	694:694	x	694:694	The relationships between anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y) and molecular weight (x) could be approximately expressed as the power function (y = a × xb), and these activity potencies of dHG-5 were approximately equivalent to the weighted average sum of that of its oligosaccharides.
32942073	4	41	theme	molecular	676:684	arg1	weight					686:691	molecular weight	676:691	molecular weight (x)	676:695	The relationships between anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y) and molecular weight (x) could be approximately expressed as the power function (y = a × xb), and these activity potencies of dHG-5 were approximately equivalent to the weighted average sum of that of its oligosaccharides.
32942073	4	42	theme	power	737:741	arg1	y = a × xb					753:762	y = a × xb	753:762	y = a × xb	753:762	The relationships between anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y) and molecular weight (x) could be approximately expressed as the power function (y = a × xb), and these activity potencies of dHG-5 were approximately equivalent to the weighted average sum of that of its oligosaccharides.
32942073	4	42	theme	power	737:741	arg1	function					743:750	the power function	733:750	the power function (y = a × xb)	733:763	The relationships between anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y) and molecular weight (x) could be approximately expressed as the power function (y = a × xb), and these activity potencies of dHG-5 were approximately equivalent to the weighted average sum of that of its oligosaccharides.
32942073	4	42	theme	power	737:741	arg1	relationships					575:587	The relationships	571:587	The relationships between anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y) and molecular weight (x)	571:695	The relationships between anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y) and molecular weight (x) could be approximately expressed as the power function (y = a × xb), and these activity potencies of dHG-5 were approximately equivalent to the weighted average sum of that of its oligosaccharides.
32942073	4	43	theme	weighted	841:848	arg1	sum					858:860	the weighted average sum	837:860	the weighted average sum of that of its oligosaccharides	837:892	The relationships between anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y) and molecular weight (x) could be approximately expressed as the power function (y = a × xb), and these activity potencies of dHG-5 were approximately equivalent to the weighted average sum of that of its oligosaccharides.
32942073	2	44	dep	cucumber	305:312	arg1	fuscopunctata					325:337	sea cucumber Holothuria fuscopunctata	301:337	sea cucumber Holothuria fuscopunctata	301:337	A depolymerized fraction of fucosylated glycosaminoglycan from sea cucumber Holothuria fuscopunctata, dHG-5 (Mw 5.2 kDa), showed potent and selective inhibition of iXase (IC50, 14 nM).
32942073	5	45	theme	pharmacological	915:929	arg1	properties					931:940	the prominent pharmacological properties	901:940	the prominent pharmacological properties	901:940	Given the prominent pharmacological properties, well-defined chemical composition and explicable relationships between dHG-5 and its oligosaccharides in pharmacological behaviors, dHG-5 is expected to be an ideal novel anticoagulant medicine.
32942073	1	46	theme	last	109:112	arg1	Xase					91:94	Intrinsic Xase	81:94	Intrinsic Xase (iXase)	81:102	Intrinsic Xase (iXase), the last and rate-limiting enzyme complex in the intrinsic coagulation pathway, may be an ideal target for antithrombotic treatment.
32942073	1	46	theme	last	109:112	arg1	complex					139:145	the last and rate-limiting enzyme complex	105:145	the last and rate-limiting enzyme complex in the intrinsic coagulation pathway	105:182	Intrinsic Xase (iXase), the last and rate-limiting enzyme complex in the intrinsic coagulation pathway, may be an ideal target for antithrombotic treatment.
32942073	1	47	theme	ideal	195:199	arg1	target					201:206	an ideal target	192:206	an ideal target for antithrombotic treatment	192:235	Intrinsic Xase (iXase), the last and rate-limiting enzyme complex in the intrinsic coagulation pathway, may be an ideal target for antithrombotic treatment.
32942073	1	47	theme	ideal	195:199	arg1	Xase					91:94	Intrinsic Xase	81:94	Intrinsic Xase (iXase)	81:102	Intrinsic Xase (iXase), the last and rate-limiting enzyme complex in the intrinsic coagulation pathway, may be an ideal target for antithrombotic treatment.
32942073	0	48	theme	candidate	64:72	arg1	dHG-5					74:78	a novel anticoagulant candidate dHG-5	42:78	a novel anticoagulant candidate dHG-5	42:78	The components and activities analysis of a novel anticoagulant candidate dHG-5.
32942073	0	49	dep	components	4:13	arg1	The					0:2	The	0:2	The	0:2	The components and activities analysis of a novel anticoagulant candidate dHG-5.
32942073	0	49	dep	components	4:13	arg1	analysis					30:37	analysis	30:37	The components and activities analysis of a novel anticoagulant candidate dHG-5.	0:79	The components and activities analysis of a novel anticoagulant candidate dHG-5.
32942073	4	50	theme	antithrombotic	642:655	arg1	activities					657:666	anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities	597:666	anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y)	597:670	The relationships between anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y) and molecular weight (x) could be approximately expressed as the power function (y = a × xb), and these activity potencies of dHG-5 were approximately equivalent to the weighted average sum of that of its oligosaccharides.
32942073	4	50	theme	antithrombotic	642:655	arg1	y					669:669	y	669:669	y	669:669	The relationships between anti-iXase, f.IXa-binding, anticoagulant and antithrombotic activities (y) and molecular weight (x) could be approximately expressed as the power function (y = a × xb), and these activity potencies of dHG-5 were approximately equivalent to the weighted average sum of that of its oligosaccharides.
32942073	0	51	theme	anticoagulant	50:62	arg1	dHG-5					74:78	a novel anticoagulant candidate dHG-5	42:78	a novel anticoagulant candidate dHG-5	42:78	The components and activities analysis of a novel anticoagulant candidate dHG-5.
32942073	3	52	theme	MS	559:560	arg1	spectra					562:568	NMR and MS spectra	551:568	NMR and MS spectra	551:568	In this work, the series of oligosaccharides contained in dHG-5 were purified and their precise structures were confirmed by 2D NMR and MS spectra.
32942073	1	53	theme	rate-limiting	118:130	arg1	Xase					91:94	Intrinsic Xase	81:94	Intrinsic Xase (iXase)	81:102	Intrinsic Xase (iXase), the last and rate-limiting enzyme complex in the intrinsic coagulation pathway, may be an ideal target for antithrombotic treatment.
32942073	1	53	theme	rate-limiting	118:130	arg1	complex					139:145	the last and rate-limiting enzyme complex	105:145	the last and rate-limiting enzyme complex in the intrinsic coagulation pathway	105:182	Intrinsic Xase (iXase), the last and rate-limiting enzyme complex in the intrinsic coagulation pathway, may be an ideal target for antithrombotic treatment.
33429231	0	0	theme	hexavalent	93:102	arg1	chromium					104:111	hexavalent chromium	93:111	hexavalent chromium	93:111	Metal-organic frameworks/alginate composite beads as effective adsorbents for the removal of hexavalent chromium from aqueous solution.
33429231	6	1	theme	Cr	856:857	arg1	experiment					842:851	The desorption experiment	827:851	The desorption experiment of Cr(VI) adsorbed UiO-66@ABs (82%)	827:887	The desorption experiment of Cr(VI) adsorbed UiO-66@ABs (82%) demonstrates satisfactory regeneration efficiency.
33429231	4	2	theme	second	631:636	arg1	order					638:642	pseudo second order	624:642	pseudo second order	624:642	The adsorption kinetics follows pseudo second order and the equilibrium isotherm is consistent with Langmuir isotherm model.
33429231	6	3	theme	satisfactory	902:913	arg1	efficiency					928:937	satisfactory regeneration efficiency	902:937	satisfactory regeneration efficiency	902:937	The desorption experiment of Cr(VI) adsorbed UiO-66@ABs (82%) demonstrates satisfactory regeneration efficiency.
33429231	7	4	from	application	1057:1067	arg1	Cr					1072:1073	Cr(VI) removal from wastewater	1072:1101	Cr(VI) removal from wastewater	1072:1101	Based on our findings and comparative controlled experiments, the superiority of UiO-66@ABs promises their potential application in Cr(VI) removal from wastewater.
33429231	7	4	from	application	1057:1067	arg1	wastewater					1092:1101	wastewater	1092:1101	wastewater	1092:1101	Based on our findings and comparative controlled experiments, the superiority of UiO-66@ABs promises their potential application in Cr(VI) removal from wastewater.
33429231	7	5	theme	ABs	1028:1030	arg1	superiority					1006:1016	the superiority	1002:1016	the superiority of UiO-66@ABs	1002:1030	Based on our findings and comparative controlled experiments, the superiority of UiO-66@ABs promises their potential application in Cr(VI) removal from wastewater.
33429231	2	6	theme	potential	345:353	arg1	adsorbents					355:364	potential adsorbents	345:364	potential adsorbents for Cr(VI)	345:375	This study investigates the role of metal-organic frameworks (MOFs) doped alginate beads (MOFs@ABs) as potential adsorbents for Cr(VI).
33429231	7	7	theme	@	1027:1027	arg1	ABs					1028:1030	UiO-66@ABs	1021:1030	UiO-66@ABs	1021:1030	Based on our findings and comparative controlled experiments, the superiority of UiO-66@ABs promises their potential application in Cr(VI) removal from wastewater.
33429231	4	8	theme	isotherm	701:708	arg1	model					710:714	Langmuir isotherm model	692:714	Langmuir isotherm model	692:714	The adsorption kinetics follows pseudo second order and the equilibrium isotherm is consistent with Langmuir isotherm model.
33429231	2	9	theme	MOFs	332:335	arg1	beads					325:329	alginate beads	316:329	alginate beads (MOFs@ABs)	316:340	This study investigates the role of metal-organic frameworks (MOFs) doped alginate beads (MOFs@ABs) as potential adsorbents for Cr(VI).
33429231	2	9	theme	MOFs	332:335	arg1	ABs					337:339	MOFs@ABs	332:339	MOFs@ABs	332:339	This study investigates the role of metal-organic frameworks (MOFs) doped alginate beads (MOFs@ABs) as potential adsorbents for Cr(VI).
33429231	6	10	theme	desorption	831:840	arg1	experiment					842:851	The desorption experiment	827:851	The desorption experiment of Cr(VI) adsorbed UiO-66@ABs (82%)	827:887	The desorption experiment of Cr(VI) adsorbed UiO-66@ABs (82%) demonstrates satisfactory regeneration efficiency.
33429231	4	11	theme	Langmuir	692:699	arg1	model					710:714	Langmuir isotherm model	692:714	Langmuir isotherm model	692:714	The adsorption kinetics follows pseudo second order and the equilibrium isotherm is consistent with Langmuir isotherm model.
33429231	4	12	theme	equilibrium	652:662	arg1	consistent					676:685	consistent	676:685	consistent	676:685	The adsorption kinetics follows pseudo second order and the equilibrium isotherm is consistent with Langmuir isotherm model.
33429231	4	12	theme	equilibrium	652:662	arg1	isotherm					664:671	the equilibrium isotherm	648:671	the equilibrium isotherm	648:671	The adsorption kinetics follows pseudo second order and the equilibrium isotherm is consistent with Langmuir isotherm model.
33429231	6	13	theme	regeneration	915:926	arg1	efficiency					928:937	satisfactory regeneration efficiency	902:937	satisfactory regeneration efficiency	902:937	The desorption experiment of Cr(VI) adsorbed UiO-66@ABs (82%) demonstrates satisfactory regeneration efficiency.
33429231	4	14	theme	pseudo	624:629	arg1	order					638:642	pseudo second order	624:642	pseudo second order	624:642	The adsorption kinetics follows pseudo second order and the equilibrium isotherm is consistent with Langmuir isotherm model.
33429231	3	15	theme	concentration	436:448	arg1	Effects					378:384	Effects	378:384	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption	378:491	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution.
33429231	2	16	dep	Cr	370:371	arg1	VI					373:374	VI	373:374	VI	373:374	This study investigates the role of metal-organic frameworks (MOFs) doped alginate beads (MOFs@ABs) as potential adsorbents for Cr(VI).
33429231	4	17	theme	adsorption	596:605	arg1	kinetics					607:614	The adsorption kinetics	592:614	The adsorption kinetics	592:614	The adsorption kinetics follows pseudo second order and the equilibrium isotherm is consistent with Langmuir isotherm model.
33429231	1	18	theme	potable	192:198	arg1	bodies					206:211	potable water bodies	192:211	potable water bodies	192:211	Industrial waste discharge comprising heavy metals into potable water bodies induces many health hazards.
33429231	0	19	theme	aqueous	118:124	arg1	solution					126:133	aqueous solution	118:133	aqueous solution	118:133	Metal-organic frameworks/alginate composite beads as effective adsorbents for the removal of hexavalent chromium from aqueous solution.
33429231	6	20	dep	Cr	856:857	arg1	ABs					879:881	adsorbed UiO-66@ABs	863:881	Cr(VI) adsorbed UiO-66@ABs (82%)	856:887	The desorption experiment of Cr(VI) adsorbed UiO-66@ABs (82%) demonstrates satisfactory regeneration efficiency.
33429231	6	20	dep	Cr	856:857	arg1	VI					859:860	VI	859:860	VI	859:860	The desorption experiment of Cr(VI) adsorbed UiO-66@ABs (82%) demonstrates satisfactory regeneration efficiency.
33429231	3	21	theme	pH	389:390	arg1	Effects					378:384	Effects	378:384	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption	378:491	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution.
33429231	1	22	theme	water	200:204	arg1	bodies					206:211	potable water bodies	192:211	potable water bodies	192:211	Industrial waste discharge comprising heavy metals into potable water bodies induces many health hazards.
33429231	6	23	theme	@	878:878	arg1	ABs					879:881	adsorbed UiO-66@ABs	863:881	Cr(VI) adsorbed UiO-66@ABs (82%)	856:887	The desorption experiment of Cr(VI) adsorbed UiO-66@ABs (82%) demonstrates satisfactory regeneration efficiency.
33429231	3	24	dep	pH	389:390	arg1	dosage					465:470	dosage	465:470	dosage	465:470	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution.
33429231	3	25	theme	adsorption	517:526	arg1	ability					528:534	adsorption ability	517:534	adsorption ability of UiO-66@ABs	517:548	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution.
33429231	3	26	theme	stirring	393:400	arg1	rate					402:405	stirring rate	393:405	stirring rate	393:405	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution.
33429231	5	27	theme	UiO-66	752:757	arg1	ABs					759:761	UiO-66@ABs	752:761	UiO-66@ABs calculated from the model	752:787	The maximum adsorption capacity of UiO-66@ABs calculated from the model conforms to the experimental results.
33429231	7	28	dep	Cr	1072:1073	arg1	VI					1075:1076	VI	1075:1076	VI	1075:1076	Based on our findings and comparative controlled experiments, the superiority of UiO-66@ABs promises their potential application in Cr(VI) removal from wastewater.
33429231	7	28	dep	Cr	1072:1073	arg1	removal					1079:1085	removal	1079:1085	Cr(VI) removal from wastewater	1072:1101	Based on our findings and comparative controlled experiments, the superiority of UiO-66@ABs promises their potential application in Cr(VI) removal from wastewater.
33429231	3	29	theme	rate	402:405	arg1	Effects					378:384	Effects	378:384	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption	378:491	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution.
33429231	5	30	theme	@	758:758	arg1	ABs					759:761	UiO-66@ABs	752:761	UiO-66@ABs calculated from the model	752:787	The maximum adsorption capacity of UiO-66@ABs calculated from the model conforms to the experimental results.
33429231	7	31	theme	UiO-66	1021:1026	arg1	ABs					1028:1030	UiO-66@ABs	1021:1030	UiO-66@ABs	1021:1030	Based on our findings and comparative controlled experiments, the superiority of UiO-66@ABs promises their potential application in Cr(VI) removal from wastewater.
33429231	1	32	theme	many	221:224	arg1	hazards					233:239	many health hazards	221:239	many health hazards	221:239	Industrial waste discharge comprising heavy metals into potable water bodies induces many health hazards.
33429231	0	33	theme	composite	34:42	arg1	beads					44:48	composite beads	34:48	composite beads	34:48	Metal-organic frameworks/alginate composite beads as effective adsorbents for the removal of hexavalent chromium from aqueous solution.
33429231	0	33	theme	composite	34:42	arg1	adsorbents					63:72	effective adsorbents	53:72	effective adsorbents for the removal of hexavalent chromium from aqueous solution	53:133	Metal-organic frameworks/alginate composite beads as effective adsorbents for the removal of hexavalent chromium from aqueous solution.
33429231	2	34	theme	alginate	316:323	arg1	beads					325:329	alginate beads	316:329	alginate beads (MOFs@ABs)	316:340	This study investigates the role of metal-organic frameworks (MOFs) doped alginate beads (MOFs@ABs) as potential adsorbents for Cr(VI).
33429231	2	34	theme	alginate	316:323	arg1	ABs					337:339	MOFs@ABs	332:339	MOFs@ABs	332:339	This study investigates the role of metal-organic frameworks (MOFs) doped alginate beads (MOFs@ABs) as potential adsorbents for Cr(VI).
33429231	1	35	theme	health	226:231	arg1	hazards					233:239	many health hazards	221:239	many health hazards	221:239	Industrial waste discharge comprising heavy metals into potable water bodies induces many health hazards.
33429231	0	36	theme	effective	53:61	arg1	beads					44:48	composite beads	34:48	composite beads	34:48	Metal-organic frameworks/alginate composite beads as effective adsorbents for the removal of hexavalent chromium from aqueous solution.
33429231	0	36	theme	effective	53:61	arg1	adsorbents					63:72	effective adsorbents	53:72	effective adsorbents for the removal of hexavalent chromium from aqueous solution	53:133	Metal-organic frameworks/alginate composite beads as effective adsorbents for the removal of hexavalent chromium from aqueous solution.
33429231	3	37	dep	adsorption	482:491	arg1	VI					478:479	VI	478:479	VI	478:479	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution.
33429231	7	38	theme	comparative	966:976	arg1	experiments					989:999	comparative controlled experiments	966:999	comparative controlled experiments	966:999	Based on our findings and comparative controlled experiments, the superiority of UiO-66@ABs promises their potential application in Cr(VI) removal from wastewater.
33429231	3	39	theme	aqueous	574:580	arg1	solution					582:589	aqueous solution	574:589	aqueous solution	574:589	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution.
33429231	3	40	theme	@	545:545	arg1	ABs					546:548	UiO-66@ABs	539:548	UiO-66@ABs	539:548	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution.
33429231	5	41	theme	experimental	805:816	arg1	results					818:824	the experimental results	801:824	the experimental results	801:824	The maximum adsorption capacity of UiO-66@ABs calculated from the model conforms to the experimental results.
33429231	5	42	theme	maximum	721:727	arg1	capacity					740:747	The maximum adsorption capacity	717:747	The maximum adsorption capacity of UiO-66@ABs calculated from the model	717:787	The maximum adsorption capacity of UiO-66@ABs calculated from the model conforms to the experimental results.
33429231	6	43	theme	UiO-66	872:877	arg1	ABs					879:881	adsorbed UiO-66@ABs	863:881	Cr(VI) adsorbed UiO-66@ABs (82%)	856:887	The desorption experiment of Cr(VI) adsorbed UiO-66@ABs (82%) demonstrates satisfactory regeneration efficiency.
33429231	7	44	theme	controlled	978:987	arg1	experiments					989:999	comparative controlled experiments	966:999	comparative controlled experiments	966:999	Based on our findings and comparative controlled experiments, the superiority of UiO-66@ABs promises their potential application in Cr(VI) removal from wastewater.
33429231	3	45	theme	ABs	546:548	arg1	ability					528:534	adsorption ability	517:534	adsorption ability of UiO-66@ABs	517:548	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution.
33429231	3	46	theme	initial	421:427	arg1	concentration					436:448	initial chrome concentration	421:448	initial chrome concentration	421:448	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution.
33429231	5	47	theme	adsorption	729:738	arg1	capacity					740:747	The maximum adsorption capacity	717:747	The maximum adsorption capacity of UiO-66@ABs calculated from the model	717:787	The maximum adsorption capacity of UiO-66@ABs calculated from the model conforms to the experimental results.
33429231	4	48	with	consistent	676:685	arg1	model					710:714	Langmuir isotherm model	692:714	Langmuir isotherm model	692:714	The adsorption kinetics follows pseudo second order and the equilibrium isotherm is consistent with Langmuir isotherm model.
33429231	5	49	theme	ABs	759:761	arg1	capacity					740:747	The maximum adsorption capacity	717:747	The maximum adsorption capacity of UiO-66@ABs calculated from the model	717:787	The maximum adsorption capacity of UiO-66@ABs calculated from the model conforms to the experimental results.
33429231	3	50	theme	particles	455:463	arg1	Effects					378:384	Effects	378:384	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption	378:491	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution.
33429231	7	51	from	wastewater	1092:1101	arg1	Cr					1072:1073	Cr(VI) removal from wastewater	1072:1101	Cr(VI) removal from wastewater	1072:1101	Based on our findings and comparative controlled experiments, the superiority of UiO-66@ABs promises their potential application in Cr(VI) removal from wastewater.
33429231	7	51	from	wastewater	1092:1101	arg1	application					1057:1067	their potential application	1041:1067	their potential application in Cr(VI) removal from wastewater	1041:1101	Based on our findings and comparative controlled experiments, the superiority of UiO-66@ABs promises their potential application in Cr(VI) removal from wastewater.
33429231	0	52	from	solution	126:133	arg1	removal					82:88	the removal	78:88	the removal of hexavalent chromium from aqueous solution	78:133	Metal-organic frameworks/alginate composite beads as effective adsorbents for the removal of hexavalent chromium from aqueous solution.
33429231	3	53	theme	UiO-66	539:544	arg1	ABs					546:548	UiO-66@ABs	539:548	UiO-66@ABs	539:548	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution.
33429231	3	54	theme	Cr	475:476	arg1	adsorption					482:491	Cr(VI) adsorption	475:491	Cr(VI) adsorption	475:491	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution.
33429231	1	55	theme	Industrial	136:145	arg1	discharge					153:161	Industrial waste discharge	136:161	Industrial waste discharge comprising heavy metals into potable water bodies	136:211	Industrial waste discharge comprising heavy metals into potable water bodies induces many health hazards.
33429231	2	56	theme	frameworks	292:301	arg1	role					270:273	the role	266:273	the role of metal-organic frameworks (MOFs) doped alginate beads (MOFs@ABs) as potential adsorbents for Cr(VI)	266:375	This study investigates the role of metal-organic frameworks (MOFs) doped alginate beads (MOFs@ABs) as potential adsorbents for Cr(VI).
33429231	1	57	theme	waste	147:151	arg1	discharge					153:161	Industrial waste discharge	136:161	Industrial waste discharge comprising heavy metals into potable water bodies	136:211	Industrial waste discharge comprising heavy metals into potable water bodies induces many health hazards.
33429231	2	58	theme	metal-organic	278:290	arg1	MOFs					304:307	MOFs	304:307	MOFs	304:307	This study investigates the role of metal-organic frameworks (MOFs) doped alginate beads (MOFs@ABs) as potential adsorbents for Cr(VI).
33429231	2	58	theme	metal-organic	278:290	arg1	frameworks					292:301	metal-organic frameworks	278:301	metal-organic frameworks (MOFs) doped alginate beads (MOFs@ABs)	278:340	This study investigates the role of metal-organic frameworks (MOFs) doped alginate beads (MOFs@ABs) as potential adsorbents for Cr(VI).
33429231	2	59	theme	@	336:336	arg1	beads					325:329	alginate beads	316:329	alginate beads (MOFs@ABs)	316:340	This study investigates the role of metal-organic frameworks (MOFs) doped alginate beads (MOFs@ABs) as potential adsorbents for Cr(VI).
33429231	2	59	theme	@	336:336	arg1	ABs					337:339	MOFs@ABs	332:339	MOFs@ABs	332:339	This study investigates the role of metal-organic frameworks (MOFs) doped alginate beads (MOFs@ABs) as potential adsorbents for Cr(VI).
33429231	3	60	dep	removal	561:567	arg1	VI					557:558	VI	557:558	VI	557:558	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution.
33429231	3	61	from	solution	582:589	arg1	removal					561:567	Cr(VI) removal	554:567	Cr(VI) removal from aqueous solution	554:589	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution.
33429231	3	62	theme	chrome	429:434	arg1	concentration					436:448	initial chrome concentration	421:448	initial chrome concentration	421:448	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution.
33429231	3	63	theme	temperature	408:418	arg1	Effects					378:384	Effects	378:384	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption	378:491	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution.
33429231	7	64	theme	potential	1047:1055	arg1	application					1057:1067	their potential application	1041:1067	their potential application in Cr(VI) removal from wastewater	1041:1101	Based on our findings and comparative controlled experiments, the superiority of UiO-66@ABs promises their potential application in Cr(VI) removal from wastewater.
33429231	3	65	from	Effects	378:384	arg1	adsorption					482:491	Cr(VI) adsorption	475:491	Cr(VI) adsorption	475:491	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution.
33429231	3	66	theme	Cr	554:555	arg1	removal					561:567	Cr(VI) removal	554:567	Cr(VI) removal from aqueous solution	554:589	Effects of pH, stirring rate, temperature, initial chrome concentration, and particles dosage on Cr(VI) adsorption are studied to evaluate adsorption ability of UiO-66@ABs for Cr(VI) removal from aqueous solution.
33429231	1	67	theme	heavy	174:178	arg1	metals					180:185	heavy metals	174:185	heavy metals	174:185	Industrial waste discharge comprising heavy metals into potable water bodies induces many health hazards.
33429231	6	68	theme	adsorbed	863:870	arg1	ABs					879:881	adsorbed UiO-66@ABs	863:881	Cr(VI) adsorbed UiO-66@ABs (82%)	856:887	The desorption experiment of Cr(VI) adsorbed UiO-66@ABs (82%) demonstrates satisfactory regeneration efficiency.
33429231	0	69	theme	chromium	104:111	arg1	removal					82:88	the removal	78:88	the removal of hexavalent chromium from aqueous solution	78:133	Metal-organic frameworks/alginate composite beads as effective adsorbents for the removal of hexavalent chromium from aqueous solution.
33030698	10	0	theme	chief	1374:1378	arg1	cells					1380:1384	the chief cells	1370:1384	the chief cells	1370:1384	The level of glycosylation of the chief cells was lower than that of the mucous cells.
33030698	2	1	theme	lower	344:348	arg1	glycosylation					350:362	a lower glycosylation	342:362	a lower glycosylation	342:362	However, the chief cells of these glands have been largely ignored because they secrete mainly zymogens with a lower glycosylation.
33030698	7	2	theme	cell	1097:1100	arg1	membrane					1102:1109	the apical cell membrane	1086:1109	the apical cell membrane	1086:1109	In addition, the presence of O-glycans in the apical cell membrane was demonstrated with the lectins AAL, HPA, MPA/MPL, PNA, RCA-I, and WGA.
33030698	3	3	theme	lectins	477:483	arg1	battery					453:459	a battery	451:459	a battery of 17 different lectins, recognizing Fucose, N-acetylgalactosamine, Galactose, N-acetylneuraminic acid, N-acetylglucosamine and Mannose containing oligosaccharides	451:623	The aim of this work was to analyze the glycoconjugates of the gastric chief cells by a battery of 17 different lectins, recognizing Fucose, N-acetylgalactosamine, Galactose, N-acetylneuraminic acid, N-acetylglucosamine and Mannose containing oligosaccharides.
33030698	1	4	theme	glycoconjugates	151:165	arg1	composition					132:142	the composition	128:142	the composition of the glycoconjugates of the mucus-secreting cells of the fundic glands of the stomach	128:230	Many studies have been conducted to determine the composition of the glycoconjugates of the mucus-secreting cells of the fundic glands of the stomach.
33030698	7	5	theme	O-glycans	1073:1081	arg1	presence					1061:1068	the presence	1057:1068	the presence of O-glycans in the apical cell membrane	1057:1109	In addition, the presence of O-glycans in the apical cell membrane was demonstrated with the lectins AAL, HPA, MPA/MPL, PNA, RCA-I, and WGA.
33030698	1	6	theme	cells	190:194	arg1	glycoconjugates					151:165	the glycoconjugates	147:165	the glycoconjugates of the mucus-secreting cells of the fundic glands of the stomach	147:230	Many studies have been conducted to determine the composition of the glycoconjugates of the mucus-secreting cells of the fundic glands of the stomach.
33030698	8	7	theme	β-elimination	1238:1250	arg1	pre-treatments					1252:1265	short-term β-elimination pre-treatments	1227:1265	short-term β-elimination pre-treatments	1227:1265	Some of these O-glycans were resistant to short-term β-elimination pre-treatments.
33030698	13	8	theme	cell	1689:1692	arg1	glycoconjugates					1694:1708	gastric chief cell glycoconjugates	1675:1708	gastric chief cell glycoconjugates	1675:1708	The knowledge of gastric chief cell glycoconjugates is relevant because of their potential involvement not only in in physiological but also in pathological processes, such as cancer.
33030698	4	9	link	N-linked	844:851	arg1	oligosaccharides					853:868	N-linked oligosaccharides	844:868	N-linked oligosaccharides	844:868	Histochemical techniques were performed with several lectins and also combined with two pre-treatments; β-elimination, which removes O-linked oligosaccharides, and incubation with Peptide-N-Gycosidase F, which removes N-linked oligosaccharides.
33030698	4	10	with	β-elimination	730:742	arg1	F					827:827	Peptide-N-Gycosidase F	806:827	Peptide-N-Gycosidase F	806:827	Histochemical techniques were performed with several lectins and also combined with two pre-treatments; β-elimination, which removes O-linked oligosaccharides, and incubation with Peptide-N-Gycosidase F, which removes N-linked oligosaccharides.
33030698	3	11	theme	Mannose	589:595	arg1	oligosaccharides					608:623	Mannose containing oligosaccharides	589:623	Mannose containing oligosaccharides	589:623	The aim of this work was to analyze the glycoconjugates of the gastric chief cells by a battery of 17 different lectins, recognizing Fucose, N-acetylgalactosamine, Galactose, N-acetylneuraminic acid, N-acetylglucosamine and Mannose containing oligosaccharides.
33030698	6	12	theme	chief	1031:1035	arg1	cells					1037:1041	the chief cells	1027:1041	the chief cells	1027:1041	Many lectins did not stain the chief cells.
33030698	11	13	theme	O-glycans	1443:1451	arg1	consistent					1484:1493	consistent	1484:1493	consistent	1484:1493	The presence of O-glycans in the apical cell membrane is consistent with the presence of mucins such as MUC1 in the apical membrane of chief cells.
33030698	11	13	theme	O-glycans	1443:1451	arg1	presence					1431:1438	The presence	1427:1438	The presence of O-glycans in the apical cell membrane	1427:1479	The presence of O-glycans in the apical cell membrane is consistent with the presence of mucins such as MUC1 in the apical membrane of chief cells.
33030698	12	14	theme	basal	1641:1645	arg1	cytoplasm					1647:1655	the basal cytoplasm	1637:1655	the basal cytoplasm	1637:1655	Moreover, Mannose-binding lectins revealed N-glycosylation in the basal cytoplasm.
33030698	10	15	theme	mucous	1413:1418	arg1	cells					1420:1424	the mucous cells	1409:1424	the mucous cells	1409:1424	The level of glycosylation of the chief cells was lower than that of the mucous cells.
33030698	0	16	theme	fundic	59:64	arg1	glands					74:79	the rat fundic gastric glands	51:79	the rat fundic gastric glands	51:79	Identification of sugar moieties in chief cells of the rat fundic gastric glands.
33030698	9	17	theme	Mannose-binding	1268:1282	arg1	lectins					1284:1290	Mannose-binding lectins	1268:1290	Mannose-binding lectins	1268:1290	Mannose-binding lectins stained the basal cytoplasm of the chief cells.
33030698	5	18	theme	WGA	921:923	arg1	histochemistry					925:938	WGA histochemistry	921:938	WGA histochemistry	921:938	In addition, acid hydrolysis was performed before WGA histochemistry, and incubation with glucose oxidase before Con A labeling.
33030698	0	19	from	Identification	0:13	arg1	cells					42:46	chief cells	36:46	chief cells of the rat fundic gastric glands	36:79	Identification of sugar moieties in chief cells of the rat fundic gastric glands.
33030698	4	20	theme	O-linked	759:766	arg1	oligosaccharides					768:783	O-linked oligosaccharides	759:783	O-linked oligosaccharides	759:783	Histochemical techniques were performed with several lectins and also combined with two pre-treatments; β-elimination, which removes O-linked oligosaccharides, and incubation with Peptide-N-Gycosidase F, which removes N-linked oligosaccharides.
33030698	11	21	theme	cell	1467:1470	arg1	membrane					1472:1479	the apical cell membrane	1456:1479	the apical cell membrane	1456:1479	The presence of O-glycans in the apical cell membrane is consistent with the presence of mucins such as MUC1 in the apical membrane of chief cells.
33030698	4	22	with	incubation	790:799	arg1	F					827:827	Peptide-N-Gycosidase F	806:827	Peptide-N-Gycosidase F	806:827	Histochemical techniques were performed with several lectins and also combined with two pre-treatments; β-elimination, which removes O-linked oligosaccharides, and incubation with Peptide-N-Gycosidase F, which removes N-linked oligosaccharides.
33030698	0	23	theme	glands	74:79	arg1	cells					42:46	chief cells	36:46	chief cells of the rat fundic gastric glands	36:79	Identification of sugar moieties in chief cells of the rat fundic gastric glands.
33030698	5	24	theme	A	988:988	arg1	labeling					990:997	Con A labeling	984:997	Con A labeling	984:997	In addition, acid hydrolysis was performed before WGA histochemistry, and incubation with glucose oxidase before Con A labeling.
33030698	6	25	theme	Many	1000:1003	arg1	lectins					1005:1011	Many lectins	1000:1011	Many lectins	1000:1011	Many lectins did not stain the chief cells.
33030698	2	26	gly	glycosylation	350:362	arg1	zymogens					328:335	zymogens	328:335	zymogens with a lower glycosylation	328:362	However, the chief cells of these glands have been largely ignored because they secrete mainly zymogens with a lower glycosylation.
33030698	9	27	theme	basal	1304:1308	arg1	cytoplasm					1310:1318	the basal cytoplasm	1300:1318	the basal cytoplasm of the chief cells	1300:1337	Mannose-binding lectins stained the basal cytoplasm of the chief cells.
33030698	3	28	theme	work	381:384	arg1	aim					369:371	The aim	365:371	The aim of this work	365:384	The aim of this work was to analyze the glycoconjugates of the gastric chief cells by a battery of 17 different lectins, recognizing Fucose, N-acetylgalactosamine, Galactose, N-acetylneuraminic acid, N-acetylglucosamine and Mannose containing oligosaccharides.
33030698	5	29	theme	glucose	961:967	arg1	oxidase					969:975	glucose oxidase	961:975	glucose oxidase	961:975	In addition, acid hydrolysis was performed before WGA histochemistry, and incubation with glucose oxidase before Con A labeling.
33030698	0	30	theme	sugar	18:22	arg1	moieties					24:31	sugar moieties	18:31	sugar moieties	18:31	Identification of sugar moieties in chief cells of the rat fundic gastric glands.
33030698	9	31	theme	chief	1327:1331	arg1	cells					1333:1337	the chief cells	1323:1337	the chief cells	1323:1337	Mannose-binding lectins stained the basal cytoplasm of the chief cells.
33030698	3	32	theme	different	467:475	arg1	lectins					477:483	17 different lectins	464:483	17 different lectins	464:483	The aim of this work was to analyze the glycoconjugates of the gastric chief cells by a battery of 17 different lectins, recognizing Fucose, N-acetylgalactosamine, Galactose, N-acetylneuraminic acid, N-acetylglucosamine and Mannose containing oligosaccharides.
33030698	12	33	theme	Mannose-binding	1585:1599	arg1	lectins					1601:1607	Mannose-binding lectins	1585:1607	Mannose-binding lectins	1585:1607	Moreover, Mannose-binding lectins revealed N-glycosylation in the basal cytoplasm.
33030698	12	34	gly	N-glycosylation	1618:1632	arg1	cytoplasm					1647:1655	the basal cytoplasm	1637:1655	the basal cytoplasm	1637:1655	Moreover, Mannose-binding lectins revealed N-glycosylation in the basal cytoplasm.
33030698	10	35	gly	glycosylation	1353:1365	arg1	cells					1380:1384	the chief cells	1370:1384	the chief cells	1370:1384	The level of glycosylation of the chief cells was lower than that of the mucous cells.
33030698	11	36	theme	apical	1543:1548	arg1	membrane					1550:1557	the apical membrane	1539:1557	the apical membrane of chief cells	1539:1572	The presence of O-glycans in the apical cell membrane is consistent with the presence of mucins such as MUC1 in the apical membrane of chief cells.
33030698	3	37	theme	cells	442:446	arg1	glycoconjugates					405:419	the glycoconjugates	401:419	the glycoconjugates of the gastric chief cells	401:446	The aim of this work was to analyze the glycoconjugates of the gastric chief cells by a battery of 17 different lectins, recognizing Fucose, N-acetylgalactosamine, Galactose, N-acetylneuraminic acid, N-acetylglucosamine and Mannose containing oligosaccharides.
33030698	13	38	dep	only	1765:1768	arg1	in					1770:1771	in	1770:1771	in	1770:1771	The knowledge of gastric chief cell glycoconjugates is relevant because of their potential involvement not only in in physiological but also in pathological processes, such as cancer.
33030698	11	39	from	presence	1431:1438	arg1	membrane					1472:1479	the apical cell membrane	1456:1479	the apical cell membrane	1456:1479	The presence of O-glycans in the apical cell membrane is consistent with the presence of mucins such as MUC1 in the apical membrane of chief cells.
33030698	3	40	theme	gastric	428:434	arg1	cells					442:446	the gastric chief cells	424:446	the gastric chief cells	424:446	The aim of this work was to analyze the glycoconjugates of the gastric chief cells by a battery of 17 different lectins, recognizing Fucose, N-acetylgalactosamine, Galactose, N-acetylneuraminic acid, N-acetylglucosamine and Mannose containing oligosaccharides.
33030698	1	41	theme	fundic	203:208	arg1	glands					210:215	the fundic glands	199:215	the fundic glands of the stomach	199:230	Many studies have been conducted to determine the composition of the glycoconjugates of the mucus-secreting cells of the fundic glands of the stomach.
33030698	4	42	theme	Peptide-N-Gycosidase	806:825	arg1	F					827:827	Peptide-N-Gycosidase F	806:827	Peptide-N-Gycosidase F	806:827	Histochemical techniques were performed with several lectins and also combined with two pre-treatments; β-elimination, which removes O-linked oligosaccharides, and incubation with Peptide-N-Gycosidase F, which removes N-linked oligosaccharides.
33030698	11	43	theme	cells	1568:1572	arg1	membrane					1550:1557	the apical membrane	1539:1557	the apical membrane of chief cells	1539:1572	The presence of O-glycans in the apical cell membrane is consistent with the presence of mucins such as MUC1 in the apical membrane of chief cells.
33030698	1	44	theme	stomach	224:230	arg1	glands					210:215	the fundic glands	199:215	the fundic glands of the stomach	199:230	Many studies have been conducted to determine the composition of the glycoconjugates of the mucus-secreting cells of the fundic glands of the stomach.
33030698	7	45	from	presence	1061:1068	arg1	membrane					1102:1109	the apical cell membrane	1086:1109	the apical cell membrane	1086:1109	In addition, the presence of O-glycans in the apical cell membrane was demonstrated with the lectins AAL, HPA, MPA/MPL, PNA, RCA-I, and WGA.
33030698	13	46	theme	potential	1739:1747	arg1	involvement					1749:1759	their potential involvement	1733:1759	their potential involvement not only in in physiological but also in pathological processes, such as cancer	1733:1839	The knowledge of gastric chief cell glycoconjugates is relevant because of their potential involvement not only in in physiological but also in pathological processes, such as cancer.
33030698	7	47	theme	apical	1090:1095	arg1	membrane					1102:1109	the apical cell membrane	1086:1109	the apical cell membrane	1086:1109	In addition, the presence of O-glycans in the apical cell membrane was demonstrated with the lectins AAL, HPA, MPA/MPL, PNA, RCA-I, and WGA.
33030698	10	48	theme	glycosylation	1353:1365	arg1	lower					1390:1394	lower	1390:1394	lower	1390:1394	The level of glycosylation of the chief cells was lower than that of the mucous cells.
33030698	10	48	theme	glycosylation	1353:1365	arg1	level					1344:1348	The level	1340:1348	The level of glycosylation of the chief cells	1340:1384	The level of glycosylation of the chief cells was lower than that of the mucous cells.
33030698	2	49	theme	chief	246:250	arg1	cells					252:256	the chief cells	242:256	the chief cells of these glands	242:272	However, the chief cells of these glands have been largely ignored because they secrete mainly zymogens with a lower glycosylation.
33030698	4	50	theme	N-linked	844:851	arg1	oligosaccharides					853:868	N-linked oligosaccharides	844:868	N-linked oligosaccharides	844:868	Histochemical techniques were performed with several lectins and also combined with two pre-treatments; β-elimination, which removes O-linked oligosaccharides, and incubation with Peptide-N-Gycosidase F, which removes N-linked oligosaccharides.
33030698	5	51	theme	acid	884:887	arg1	hydrolysis					889:898	acid hydrolysis	884:898	acid hydrolysis	884:898	In addition, acid hydrolysis was performed before WGA histochemistry, and incubation with glucose oxidase before Con A labeling.
33030698	10	52	theme	cells	1380:1384	arg1	glycosylation					1353:1365	glycosylation	1353:1365	glycosylation of the chief cells	1353:1384	The level of glycosylation of the chief cells was lower than that of the mucous cells.
33030698	2	53	theme	glands	267:272	arg1	cells					252:256	the chief cells	242:256	the chief cells of these glands	242:272	However, the chief cells of these glands have been largely ignored because they secrete mainly zymogens with a lower glycosylation.
33030698	1	54	theme	mucus-secreting	174:188	arg1	cells					190:194	the mucus-secreting cells	170:194	the mucus-secreting cells of the fundic glands of the stomach	170:230	Many studies have been conducted to determine the composition of the glycoconjugates of the mucus-secreting cells of the fundic glands of the stomach.
33030698	11	55	attach	presence	1431:1438	arg1	membrane					1472:1479	the apical cell membrane	1456:1479	the apical cell membrane	1456:1479	The presence of O-glycans in the apical cell membrane is consistent with the presence of mucins such as MUC1 in the apical membrane of chief cells.
33030698	11	55	attach	presence	1431:1438	arg2	O-glycans					1443:1451	O-glycans	1443:1451	O-glycans	1443:1451	The presence of O-glycans in the apical cell membrane is consistent with the presence of mucins such as MUC1 in the apical membrane of chief cells.
33030698	7	56	attach	presence	1061:1068	arg2	O-glycans					1073:1081	O-glycans	1073:1081	O-glycans	1073:1081	In addition, the presence of O-glycans in the apical cell membrane was demonstrated with the lectins AAL, HPA, MPA/MPL, PNA, RCA-I, and WGA.
33030698	7	56	attach	presence	1061:1068	arg1	membrane					1102:1109	the apical cell membrane	1086:1109	the apical cell membrane	1086:1109	In addition, the presence of O-glycans in the apical cell membrane was demonstrated with the lectins AAL, HPA, MPA/MPL, PNA, RCA-I, and WGA.
33030698	13	57	theme	chief	1683:1687	arg1	glycoconjugates					1694:1708	gastric chief cell glycoconjugates	1675:1708	gastric chief cell glycoconjugates	1675:1708	The knowledge of gastric chief cell glycoconjugates is relevant because of their potential involvement not only in in physiological but also in pathological processes, such as cancer.
33030698	3	58	theme	N-acetylneuraminic	540:557	arg1	acid					559:562	N-acetylneuraminic acid	540:562	N-acetylneuraminic acid	540:562	The aim of this work was to analyze the glycoconjugates of the gastric chief cells by a battery of 17 different lectins, recognizing Fucose, N-acetylgalactosamine, Galactose, N-acetylneuraminic acid, N-acetylglucosamine and Mannose containing oligosaccharides.
33030698	8	59	theme	short-term	1227:1236	arg1	pre-treatments					1252:1265	short-term β-elimination pre-treatments	1227:1265	short-term β-elimination pre-treatments	1227:1265	Some of these O-glycans were resistant to short-term β-elimination pre-treatments.
33030698	13	60	theme	glycoconjugates	1694:1708	arg1	knowledge					1662:1670	The knowledge	1658:1670	The knowledge of gastric chief cell glycoconjugates	1658:1708	The knowledge of gastric chief cell glycoconjugates is relevant because of their potential involvement not only in in physiological but also in pathological processes, such as cancer.
33030698	13	60	theme	glycoconjugates	1694:1708	arg1	relevant					1713:1720	relevant	1713:1720	relevant	1713:1720	The knowledge of gastric chief cell glycoconjugates is relevant because of their potential involvement not only in in physiological but also in pathological processes, such as cancer.
33030698	13	61	from	involvement	1749:1759	arg1	processes					1815:1823	pathological processes	1802:1823	pathological processes	1802:1823	The knowledge of gastric chief cell glycoconjugates is relevant because of their potential involvement not only in in physiological but also in pathological processes, such as cancer.
33030698	13	61	from	involvement	1749:1759	arg1	physiological					1776:1788	physiological	1776:1788	physiological	1776:1788	The knowledge of gastric chief cell glycoconjugates is relevant because of their potential involvement not only in in physiological but also in pathological processes, such as cancer.
33030698	13	61	from	involvement	1749:1759	arg1	cancer					1834:1839	cancer	1834:1839	cancer	1834:1839	The knowledge of gastric chief cell glycoconjugates is relevant because of their potential involvement not only in in physiological but also in pathological processes, such as cancer.
33030698	11	62	theme	mucins	1516:1521	arg1	presence					1504:1511	the presence	1500:1511	the presence of mucins such as MUC1 in the apical membrane of chief cells	1500:1572	The presence of O-glycans in the apical cell membrane is consistent with the presence of mucins such as MUC1 in the apical membrane of chief cells.
33030698	0	63	theme	rat	55:57	arg1	glands					74:79	the rat fundic gastric glands	51:79	the rat fundic gastric glands	51:79	Identification of sugar moieties in chief cells of the rat fundic gastric glands.
33030698	11	64	from	MUC1	1531:1534	arg1	membrane					1550:1557	the apical membrane	1539:1557	the apical membrane of chief cells	1539:1572	The presence of O-glycans in the apical cell membrane is consistent with the presence of mucins such as MUC1 in the apical membrane of chief cells.
33030698	4	65	theme	several	671:677	arg1	lectins					679:685	several lectins	671:685	several lectins	671:685	Histochemical techniques were performed with several lectins and also combined with two pre-treatments; β-elimination, which removes O-linked oligosaccharides, and incubation with Peptide-N-Gycosidase F, which removes N-linked oligosaccharides.
33030698	0	66	theme	gastric	66:72	arg1	glands					74:79	the rat fundic gastric glands	51:79	the rat fundic gastric glands	51:79	Identification of sugar moieties in chief cells of the rat fundic gastric glands.
33030698	11	67	theme	apical	1460:1465	arg1	membrane					1472:1479	the apical cell membrane	1456:1479	the apical cell membrane	1456:1479	The presence of O-glycans in the apical cell membrane is consistent with the presence of mucins such as MUC1 in the apical membrane of chief cells.
33030698	5	68	theme	Con	984:986	arg1	A					988:988	Con A	984:988	Con A labeling	984:997	In addition, acid hydrolysis was performed before WGA histochemistry, and incubation with glucose oxidase before Con A labeling.
33030698	13	69	theme	gastric	1675:1681	arg1	glycoconjugates					1694:1708	gastric chief cell glycoconjugates	1675:1708	gastric chief cell glycoconjugates	1675:1708	The knowledge of gastric chief cell glycoconjugates is relevant because of their potential involvement not only in in physiological but also in pathological processes, such as cancer.
33030698	5	70	dep	incubation	945:954	arg1	labeling					990:997	Con A labeling	984:997	Con A labeling	984:997	In addition, acid hydrolysis was performed before WGA histochemistry, and incubation with glucose oxidase before Con A labeling.
33030698	7	71	dep	lectins	1137:1143	arg1	RCA-I					1169:1173	RCA-I	1169:1173	RCA-I	1169:1173	In addition, the presence of O-glycans in the apical cell membrane was demonstrated with the lectins AAL, HPA, MPA/MPL, PNA, RCA-I, and WGA.
33030698	7	71	dep	lectins	1137:1143	arg1	HPA					1150:1152	HPA	1150:1152	HPA	1150:1152	In addition, the presence of O-glycans in the apical cell membrane was demonstrated with the lectins AAL, HPA, MPA/MPL, PNA, RCA-I, and WGA.
33030698	7	71	dep	lectins	1137:1143	arg1	MPA/MPL					1155:1161	MPA/MPL	1155:1161	MPA/MPL	1155:1161	In addition, the presence of O-glycans in the apical cell membrane was demonstrated with the lectins AAL, HPA, MPA/MPL, PNA, RCA-I, and WGA.
33030698	7	71	dep	lectins	1137:1143	arg1	WGA					1180:1182	WGA	1180:1182	WGA	1180:1182	In addition, the presence of O-glycans in the apical cell membrane was demonstrated with the lectins AAL, HPA, MPA/MPL, PNA, RCA-I, and WGA.
33030698	7	71	dep	lectins	1137:1143	arg1	lectins					1137:1143	the lectins AAL, HPA, MPA/MPL, PNA, RCA-I, and WGA	1133:1182	the lectins AAL, HPA, MPA/MPL, PNA, RCA-I, and WGA	1133:1182	In addition, the presence of O-glycans in the apical cell membrane was demonstrated with the lectins AAL, HPA, MPA/MPL, PNA, RCA-I, and WGA.
33030698	7	71	dep	lectins	1137:1143	arg1	AAL					1145:1147	AAL	1145:1147	AAL	1145:1147	In addition, the presence of O-glycans in the apical cell membrane was demonstrated with the lectins AAL, HPA, MPA/MPL, PNA, RCA-I, and WGA.
33030698	7	71	dep	lectins	1137:1143	arg1	PNA					1164:1166	PNA	1164:1166	PNA	1164:1166	In addition, the presence of O-glycans in the apical cell membrane was demonstrated with the lectins AAL, HPA, MPA/MPL, PNA, RCA-I, and WGA.
33030698	4	72	dep	pre-treatments	714:727	arg1	pre-treatments					714:727	two pre-treatments	710:727	two pre-treatments; β-elimination, which removes O-linked oligosaccharides, and incubation with Peptide-N-Gycosidase F, which removes N-linked oligosaccharides	710:868	Histochemical techniques were performed with several lectins and also combined with two pre-treatments; β-elimination, which removes O-linked oligosaccharides, and incubation with Peptide-N-Gycosidase F, which removes N-linked oligosaccharides.
33030698	4	72	dep	pre-treatments	714:727	arg1	incubation					790:799	incubation	790:799	incubation with Peptide-N-Gycosidase F, which removes N-linked oligosaccharides	790:868	Histochemical techniques were performed with several lectins and also combined with two pre-treatments; β-elimination, which removes O-linked oligosaccharides, and incubation with Peptide-N-Gycosidase F, which removes N-linked oligosaccharides.
33030698	4	72	dep	pre-treatments	714:727	arg1	β-elimination					730:742	β-elimination	730:742	β-elimination	730:742	Histochemical techniques were performed with several lectins and also combined with two pre-treatments; β-elimination, which removes O-linked oligosaccharides, and incubation with Peptide-N-Gycosidase F, which removes N-linked oligosaccharides.
33030698	0	73	theme	moieties	24:31	arg1	Identification					0:13	Identification	0:13	Identification of sugar moieties in chief cells of the rat fundic gastric glands.	0:80	Identification of sugar moieties in chief cells of the rat fundic gastric glands.
33030698	1	74	theme	Many	82:85	arg1	studies					87:93	Many studies	82:93	Many studies	82:93	Many studies have been conducted to determine the composition of the glycoconjugates of the mucus-secreting cells of the fundic glands of the stomach.
33030698	13	75	theme	pathological	1802:1813	arg1	processes					1815:1823	pathological processes	1802:1823	pathological processes	1802:1823	The knowledge of gastric chief cell glycoconjugates is relevant because of their potential involvement not only in in physiological but also in pathological processes, such as cancer.
33030698	13	75	theme	pathological	1802:1813	arg1	cancer					1834:1839	cancer	1834:1839	cancer	1834:1839	The knowledge of gastric chief cell glycoconjugates is relevant because of their potential involvement not only in in physiological but also in pathological processes, such as cancer.
33030698	0	76	theme	chief	36:40	arg1	cells					42:46	chief cells	36:46	chief cells of the rat fundic gastric glands	36:79	Identification of sugar moieties in chief cells of the rat fundic gastric glands.
33030698	9	77	theme	cells	1333:1337	arg1	cytoplasm					1310:1318	the basal cytoplasm	1300:1318	the basal cytoplasm of the chief cells	1300:1337	Mannose-binding lectins stained the basal cytoplasm of the chief cells.
33030698	4	78	theme	Histochemical	626:638	arg1	techniques					640:649	Histochemical techniques	626:649	Histochemical techniques	626:649	Histochemical techniques were performed with several lectins and also combined with two pre-treatments; β-elimination, which removes O-linked oligosaccharides, and incubation with Peptide-N-Gycosidase F, which removes N-linked oligosaccharides.
33030698	11	79	with	consistent	1484:1493	arg1	presence					1504:1511	the presence	1500:1511	the presence of mucins such as MUC1 in the apical membrane of chief cells	1500:1572	The presence of O-glycans in the apical cell membrane is consistent with the presence of mucins such as MUC1 in the apical membrane of chief cells.
33030698	3	80	theme	containing	597:606	arg1	oligosaccharides					608:623	Mannose containing oligosaccharides	589:623	Mannose containing oligosaccharides	589:623	The aim of this work was to analyze the glycoconjugates of the gastric chief cells by a battery of 17 different lectins, recognizing Fucose, N-acetylgalactosamine, Galactose, N-acetylneuraminic acid, N-acetylglucosamine and Mannose containing oligosaccharides.
33030698	5	81	with	incubation	945:954	arg1	oxidase					969:975	glucose oxidase	961:975	glucose oxidase	961:975	In addition, acid hydrolysis was performed before WGA histochemistry, and incubation with glucose oxidase before Con A labeling.
33030698	4	82	link	O-linked	759:766	arg1	oligosaccharides					768:783	O-linked oligosaccharides	759:783	O-linked oligosaccharides	759:783	Histochemical techniques were performed with several lectins and also combined with two pre-treatments; β-elimination, which removes O-linked oligosaccharides, and incubation with Peptide-N-Gycosidase F, which removes N-linked oligosaccharides.
33030698	11	83	theme	chief	1562:1566	arg1	cells					1568:1572	chief cells	1562:1572	chief cells	1562:1572	The presence of O-glycans in the apical cell membrane is consistent with the presence of mucins such as MUC1 in the apical membrane of chief cells.
33030698	3	84	theme	chief	436:440	arg1	cells					442:446	the gastric chief cells	424:446	the gastric chief cells	424:446	The aim of this work was to analyze the glycoconjugates of the gastric chief cells by a battery of 17 different lectins, recognizing Fucose, N-acetylgalactosamine, Galactose, N-acetylneuraminic acid, N-acetylglucosamine and Mannose containing oligosaccharides.
33030698	1	85	theme	glands	210:215	arg1	cells					190:194	the mucus-secreting cells	170:194	the mucus-secreting cells of the fundic glands of the stomach	170:230	Many studies have been conducted to determine the composition of the glycoconjugates of the mucus-secreting cells of the fundic glands of the stomach.
33030698	2	86	with	zymogens	328:335	arg1	glycosylation					350:362	a lower glycosylation	342:362	a lower glycosylation	342:362	However, the chief cells of these glands have been largely ignored because they secrete mainly zymogens with a lower glycosylation.
32569469	9	0	theme	conformational	1958:1971	arg1	dynamics					1973:1980	the microsecond conformational dynamics	1942:1980	the microsecond conformational dynamics of domain III of HSA in all states except the intermediate state	1942:2045	Rod-shaped dextran-40 and spherical ficoll-70 hinder the microsecond conformational dynamics of domain III of HSA in all states except the intermediate state.
32569469	8	1	theme	active-site	1755:1765	arg1	dynamics					1767:1774	The active-site dynamics	1751:1774	The active-site dynamics of HSA	1751:1781	The active-site dynamics of HSA are altered significantly in the presence of all the three differently shaped crowders used in the study.
32569469	1	2	theme	macromolecular	279:292	arg1	crowders					294:301	macromolecular crowders	279:301	macromolecular crowders of various compositions, sizes, and shapes	279:344	One of the main differences in the intercellular environment compared to the laboratory condition is the presence of macromolecular crowders of various compositions, sizes, and shapes.
32569469	9	3	from	dynamics	1973:1980	arg1	states					2010:2015	all states	2006:2015	all states except the intermediate state	2006:2045	Rod-shaped dextran-40 and spherical ficoll-70 hinder the microsecond conformational dynamics of domain III of HSA in all states except the intermediate state.
32569469	4	4	theme	traditional	1098:1108	arg1	interpretation					1110:1123	the traditional interpretation	1094:1123	the traditional interpretation of excluded volume and soft interaction	1094:1163	A complete thermodynamic analysis suggests that the stabilization by dextran-40 and ficoll-70 occurs mainly through stabilizing entropic effect, which is somewhat counteracted by the destabilizing enthalpic effect, in line with what is expected from the traditional interpretation of excluded volume and soft interaction.
32569469	5	5	theme	entropic	1282:1289	arg1	opposite					1308:1315	opposite	1308:1315	opposite	1308:1315	Surprisingly, the destabilizing effect of PEG-35 is not through unfavorable interaction but through a destabilizing entropic effect, which is opposite to the excluded volume prediction.
32569469	5	5	theme	entropic	1282:1289	arg1	effect					1291:1296	a destabilizing entropic effect	1266:1296	a destabilizing entropic effect	1266:1296	Surprisingly, the destabilizing effect of PEG-35 is not through unfavorable interaction but through a destabilizing entropic effect, which is opposite to the excluded volume prediction.
32569469	10	6	from	mechanism	2240:2248	arg1	protein					2280:2286	protein	2280:2286	protein	2280:2286	Mesh-like PEG-35 in addition to hindering the microsecond conformational dynamics shifts the intermediate state from 40 to 30 °C. Overall, our results provide new insight into deciphering the mechanism of crowder-induced changes in protein.
32569469	2	7	theme	similar-sized	598:610	arg1	albumin					559:565	human serum albumin	547:565	human serum albumin (HSA)	547:571	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	2	7	theme	similar-sized	598:610	arg1	crowders					612:619	similar-sized crowders	598:619	similar-sized crowders	598:619	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	7	8	theme	HSA	1707:1709	arg1	domain					1693:1698	domain III	1693:1702	domain III of HSA	1693:1709	Moreover, while a two-state model can approximate the overall thermal denaturation of HSA in the absence and presence of various crowders, the thermal denaturation profile of domain III of HSA involves a distinct intermediate state.
32569469	7	8	theme	HSA	1707:1709	arg1	HSA					1707:1709	HSA	1707:1709	HSA	1707:1709	Moreover, while a two-state model can approximate the overall thermal denaturation of HSA in the absence and presence of various crowders, the thermal denaturation profile of domain III of HSA involves a distinct intermediate state.
32569469	4	9	theme	entropic	972:979	arg1	effect					981:986	stabilizing entropic effect	960:986	stabilizing entropic effect	960:986	A complete thermodynamic analysis suggests that the stabilization by dextran-40 and ficoll-70 occurs mainly through stabilizing entropic effect, which is somewhat counteracted by the destabilizing enthalpic effect, in line with what is expected from the traditional interpretation of excluded volume and soft interaction.
32569469	4	10	theme	soft	1148:1151	arg1	interaction					1153:1163	soft interaction	1148:1163	soft interaction	1148:1163	A complete thermodynamic analysis suggests that the stabilization by dextran-40 and ficoll-70 occurs mainly through stabilizing entropic effect, which is somewhat counteracted by the destabilizing enthalpic effect, in line with what is expected from the traditional interpretation of excluded volume and soft interaction.
32569469	0	11	theme	Single-Molecule	139:153	arg1	Level					155:159	the Single-Molecule Level	135:159	the Single-Molecule Level	135:159	Shape-Dependent Macromolecular Crowding on the Thermodynamics and Microsecond Conformational Dynamics of Protein Unfolding Revealed at the Single-Molecule Level.
32569469	10	12	theme	new	2207:2209	arg1	insight					2211:2217	new insight	2207:2217	new insight into deciphering the mechanism of crowder-induced changes in protein	2207:2286	Mesh-like PEG-35 in addition to hindering the microsecond conformational dynamics shifts the intermediate state from 40 to 30 °C. Overall, our results provide new insight into deciphering the mechanism of crowder-induced changes in protein.
32569469	3	13	theme	thermal	800:806	arg1	denaturation					808:819	the thermal denaturation	796:819	the thermal denaturation	796:819	We observed that dextran-40 and ficoll-70 counteract the thermal denaturation and PEG-35 assists it.
32569469	1	14	theme	laboratory	239:248	arg1	condition					250:258	the laboratory condition	235:258	the laboratory condition	235:258	One of the main differences in the intercellular environment compared to the laboratory condition is the presence of macromolecular crowders of various compositions, sizes, and shapes.
32569469	5	15	theme	unfavorable	1230:1240	arg1	interaction					1242:1252	unfavorable interaction	1230:1252	unfavorable interaction	1230:1252	Surprisingly, the destabilizing effect of PEG-35 is not through unfavorable interaction but through a destabilizing entropic effect, which is opposite to the excluded volume prediction.
32569469	9	16	theme	intermediate	2028:2039	arg1	state					2041:2045	the intermediate state	2024:2045	the intermediate state	2024:2045	Rod-shaped dextran-40 and spherical ficoll-70 hinder the microsecond conformational dynamics of domain III of HSA in all states except the intermediate state.
32569469	7	17	dep	absence	1615:1621	arg1	the					1611:1613	the	1611:1613	the	1611:1613	Moreover, while a two-state model can approximate the overall thermal denaturation of HSA in the absence and presence of various crowders, the thermal denaturation profile of domain III of HSA involves a distinct intermediate state.
32569469	7	18	theme	denaturation	1669:1680	arg1	profile					1682:1688	the thermal denaturation profile	1657:1688	the thermal denaturation profile of domain III of HSA	1657:1709	Moreover, while a two-state model can approximate the overall thermal denaturation of HSA in the absence and presence of various crowders, the thermal denaturation profile of domain III of HSA involves a distinct intermediate state.
32569469	10	19	theme	microsecond	2094:2104	arg1	dynamics					2121:2128	the microsecond conformational dynamics	2090:2128	the microsecond conformational dynamics	2090:2128	Mesh-like PEG-35 in addition to hindering the microsecond conformational dynamics shifts the intermediate state from 40 to 30 °C. Overall, our results provide new insight into deciphering the mechanism of crowder-induced changes in protein.
32569469	2	20	theme	human	547:551	arg1	albumin					559:565	human serum albumin	547:565	human serum albumin (HSA)	547:571	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	2	20	theme	human	547:551	arg1	crowders					682:689	macromolecular crowders	667:689	macromolecular crowders of different shapes	667:709	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	2	20	theme	human	547:551	arg1	crowders					612:619	similar-sized crowders	598:619	similar-sized crowders	598:619	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	2	20	theme	human	547:551	arg1	protein					586:592	the model protein	576:592	the model protein	576:592	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	2	20	theme	human	547:551	arg1	HSA					568:570	HSA	568:570	HSA	568:570	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	11	21	theme	entropy-enthalpy	2424:2439	arg1	compensation					2441:2452	the entropy-enthalpy compensation	2420:2452	the entropy-enthalpy compensation	2420:2452	Through our interpretation, we not only explain the unfavorable entropic contribution but also provide a physical basis to explain the entropy-enthalpy compensation.
32569469	5	22	theme	destabilizing	1268:1280	arg1	opposite					1308:1315	opposite	1308:1315	opposite	1308:1315	Surprisingly, the destabilizing effect of PEG-35 is not through unfavorable interaction but through a destabilizing entropic effect, which is opposite to the excluded volume prediction.
32569469	5	22	theme	destabilizing	1268:1280	arg1	effect					1291:1296	a destabilizing entropic effect	1266:1296	a destabilizing entropic effect	1266:1296	Surprisingly, the destabilizing effect of PEG-35 is not through unfavorable interaction but through a destabilizing entropic effect, which is opposite to the excluded volume prediction.
32569469	8	23	theme	crowders	1861:1868	arg1	presence					1816:1823	the presence	1812:1823	the presence of all the three differently shaped crowders used in the study	1812:1886	The active-site dynamics of HSA are altered significantly in the presence of all the three differently shaped crowders used in the study.
32569469	7	24	theme	crowders	1647:1654	arg1	presence					1627:1634	presence	1627:1634	presence	1627:1634	Moreover, while a two-state model can approximate the overall thermal denaturation of HSA in the absence and presence of various crowders, the thermal denaturation profile of domain III of HSA involves a distinct intermediate state.
32569469	7	24	theme	crowders	1647:1654	arg1	absence					1615:1621	absence	1615:1621	absence	1615:1621	Moreover, while a two-state model can approximate the overall thermal denaturation of HSA in the absence and presence of various crowders, the thermal denaturation profile of domain III of HSA involves a distinct intermediate state.
32569469	4	25	theme	enthalpic	1041:1049	arg1	effect					1051:1056	the destabilizing enthalpic effect	1023:1056	the destabilizing enthalpic effect	1023:1056	A complete thermodynamic analysis suggests that the stabilization by dextran-40 and ficoll-70 occurs mainly through stabilizing entropic effect, which is somewhat counteracted by the destabilizing enthalpic effect, in line with what is expected from the traditional interpretation of excluded volume and soft interaction.
32569469	0	26	from	Crowding	31:38	arg1	Thermodynamics					47:60	Thermodynamics	47:60	Thermodynamics	47:60	Shape-Dependent Macromolecular Crowding on the Thermodynamics and Microsecond Conformational Dynamics of Protein Unfolding Revealed at the Single-Molecule Level.
32569469	0	26	from	Crowding	31:38	arg1	Dynamics					93:100	Microsecond Conformational Dynamics	66:100	Microsecond Conformational Dynamics	66:100	Shape-Dependent Macromolecular Crowding on the Thermodynamics and Microsecond Conformational Dynamics of Protein Unfolding Revealed at the Single-Molecule Level.
32569469	10	27	theme	changes	2269:2275	arg1	mechanism					2240:2248	the mechanism	2236:2248	the mechanism of crowder-induced changes in protein	2236:2286	Mesh-like PEG-35 in addition to hindering the microsecond conformational dynamics shifts the intermediate state from 40 to 30 °C. Overall, our results provide new insight into deciphering the mechanism of crowder-induced changes in protein.
32569469	6	28	theme	crowder-induced	1432:1446	arg1	distortion					1448:1457	crowder-induced distortion	1432:1457	crowder-induced distortion	1432:1457	Our speculation is that the modulation of the associated water structure due to crowder-induced distortion plays a crucial role in modulating the entropic component.
32569469	5	29	theme	destabilizing	1184:1196	arg1	effect					1198:1203	the destabilizing effect	1180:1203	the destabilizing effect of PEG-35	1180:1213	Surprisingly, the destabilizing effect of PEG-35 is not through unfavorable interaction but through a destabilizing entropic effect, which is opposite to the excluded volume prediction.
32569469	11	30	theme	unfavorable	2341:2351	arg1	contribution					2362:2373	the unfavorable entropic contribution	2337:2373	the unfavorable entropic contribution	2337:2373	Through our interpretation, we not only explain the unfavorable entropic contribution but also provide a physical basis to explain the entropy-enthalpy compensation.
32569469	1	31	theme	differences	178:188	arg1	One					162:164	One	162:164	One	162:164	One of the main differences in the intercellular environment compared to the laboratory condition is the presence of macromolecular crowders of various compositions, sizes, and shapes.
32569469	1	31	theme	differences	178:188	arg1	presence					267:274	the presence	263:274	the presence of macromolecular crowders of various compositions, sizes, and shapes	263:344	One of the main differences in the intercellular environment compared to the laboratory condition is the presence of macromolecular crowders of various compositions, sizes, and shapes.
32569469	1	31	theme	differences	178:188	arg1	differences					178:188	the main differences	169:188	the main differences in the intercellular environment compared to the laboratory condition	169:258	One of the main differences in the intercellular environment compared to the laboratory condition is the presence of macromolecular crowders of various compositions, sizes, and shapes.
32569469	2	32	theme	macromolecular	667:680	arg1	albumin					559:565	human serum albumin	547:565	human serum albumin (HSA)	547:571	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	2	32	theme	macromolecular	667:680	arg1	crowders					682:689	macromolecular crowders	667:689	macromolecular crowders of different shapes	667:709	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	5	33	theme	excluded	1324:1331	arg1	prediction					1340:1349	the excluded volume prediction	1320:1349	the excluded volume prediction	1320:1349	Surprisingly, the destabilizing effect of PEG-35 is not through unfavorable interaction but through a destabilizing entropic effect, which is opposite to the excluded volume prediction.
32569469	0	34	theme	Unfolding	113:121	arg1	Thermodynamics					47:60	Thermodynamics	47:60	Thermodynamics	47:60	Shape-Dependent Macromolecular Crowding on the Thermodynamics and Microsecond Conformational Dynamics of Protein Unfolding Revealed at the Single-Molecule Level.
32569469	0	34	theme	Unfolding	113:121	arg1	Dynamics					93:100	Microsecond Conformational Dynamics	66:100	Microsecond Conformational Dynamics	66:100	Shape-Dependent Macromolecular Crowding on the Thermodynamics and Microsecond Conformational Dynamics of Protein Unfolding Revealed at the Single-Molecule Level.
32569469	11	35	theme	entropic	2353:2360	arg1	contribution					2362:2373	the unfavorable entropic contribution	2337:2373	the unfavorable entropic contribution	2337:2373	Through our interpretation, we not only explain the unfavorable entropic contribution but also provide a physical basis to explain the entropy-enthalpy compensation.
32569469	6	36	theme	water	1409:1413	arg1	structure					1415:1423	the associated water structure	1394:1423	the associated water structure due to crowder-induced distortion	1394:1457	Our speculation is that the modulation of the associated water structure due to crowder-induced distortion plays a crucial role in modulating the entropic component.
32569469	2	37	theme	conformational	480:493	arg1	dynamics					507:514	microsecond conformational fluctuation dynamics	468:514	microsecond conformational fluctuation dynamics	468:514	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	2	38	theme	shapes	704:709	arg1	albumin					559:565	human serum albumin	547:565	human serum albumin (HSA)	547:571	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	2	38	theme	shapes	704:709	arg1	crowders					682:689	macromolecular crowders	667:689	macromolecular crowders of different shapes	667:709	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	2	39	theme	shape	398:402	arg1	dependency					404:413	a systematic shape dependency	385:413	a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding	385:535	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	0	40	theme	Shape-Dependent	0:14	arg1	Crowding					31:38	Shape-Dependent Macromolecular Crowding	0:38	Shape-Dependent Macromolecular Crowding on the Thermodynamics and Microsecond Conformational Dynamics of Protein Unfolding	0:121	Shape-Dependent Macromolecular Crowding on the Thermodynamics and Microsecond Conformational Dynamics of Protein Unfolding Revealed at the Single-Molecule Level.
32569469	1	41	theme	various	306:312	arg1	compositions					314:325	various compositions	306:325	various compositions	306:325	One of the main differences in the intercellular environment compared to the laboratory condition is the presence of macromolecular crowders of various compositions, sizes, and shapes.
32569469	7	42	theme	distinct	1722:1729	arg1	state					1744:1748	a distinct intermediate state	1720:1748	a distinct intermediate state	1720:1748	Moreover, while a two-state model can approximate the overall thermal denaturation of HSA in the absence and presence of various crowders, the thermal denaturation profile of domain III of HSA involves a distinct intermediate state.
32569469	2	43	theme	protein	519:525	arg1	unfolding					527:535	protein unfolding	519:535	protein unfolding	519:535	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	2	44	theme	crowders	433:440	arg1	dependency					404:413	a systematic shape dependency	385:413	a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding	385:535	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	0	45	theme	Conformational	78:91	arg1	Dynamics					93:100	Microsecond Conformational Dynamics	66:100	Microsecond Conformational Dynamics	66:100	Shape-Dependent Macromolecular Crowding on the Thermodynamics and Microsecond Conformational Dynamics of Protein Unfolding Revealed at the Single-Molecule Level.
32569469	4	46	theme	thermodynamic	855:867	arg1	analysis					869:876	A complete thermodynamic analysis	844:876	A complete thermodynamic analysis	844:876	A complete thermodynamic analysis suggests that the stabilization by dextran-40 and ficoll-70 occurs mainly through stabilizing entropic effect, which is somewhat counteracted by the destabilizing enthalpic effect, in line with what is expected from the traditional interpretation of excluded volume and soft interaction.
32569469	6	47	theme	crucial	1467:1473	arg1	role					1475:1478	a crucial role	1465:1478	a crucial role	1465:1478	Our speculation is that the modulation of the associated water structure due to crowder-induced distortion plays a crucial role in modulating the entropic component.
32569469	1	48	theme	shapes	339:344	arg1	crowders					294:301	macromolecular crowders	279:301	macromolecular crowders of various compositions, sizes, and shapes	279:344	One of the main differences in the intercellular environment compared to the laboratory condition is the presence of macromolecular crowders of various compositions, sizes, and shapes.
32569469	7	49	theme	thermal	1580:1586	arg1	denaturation					1588:1599	the overall thermal denaturation	1568:1599	the overall thermal denaturation of HSA	1568:1606	Moreover, while a two-state model can approximate the overall thermal denaturation of HSA in the absence and presence of various crowders, the thermal denaturation profile of domain III of HSA involves a distinct intermediate state.
32569469	4	50	theme	excluded	1128:1135	arg1	volume					1137:1142	excluded volume	1128:1142	excluded volume	1128:1142	A complete thermodynamic analysis suggests that the stabilization by dextran-40 and ficoll-70 occurs mainly through stabilizing entropic effect, which is somewhat counteracted by the destabilizing enthalpic effect, in line with what is expected from the traditional interpretation of excluded volume and soft interaction.
32569469	11	51	theme	physical	2394:2401	arg1	basis					2403:2407	a physical basis	2392:2407	a physical basis to explain the entropy-enthalpy compensation	2392:2452	Through our interpretation, we not only explain the unfavorable entropic contribution but also provide a physical basis to explain the entropy-enthalpy compensation.
32569469	9	52	theme	microsecond	1946:1956	arg1	dynamics					1973:1980	the microsecond conformational dynamics	1942:1980	the microsecond conformational dynamics of domain III of HSA in all states except the intermediate state	1942:2045	Rod-shaped dextran-40 and spherical ficoll-70 hinder the microsecond conformational dynamics of domain III of HSA in all states except the intermediate state.
32569469	7	53	theme	two-state	1536:1544	arg1	model					1546:1550	a two-state model	1534:1550	a two-state model	1534:1550	Moreover, while a two-state model can approximate the overall thermal denaturation of HSA in the absence and presence of various crowders, the thermal denaturation profile of domain III of HSA involves a distinct intermediate state.
32569469	10	54	dep	30	2171:2172	arg1	to					2168:2169	to	2168:2169	to	2168:2169	Mesh-like PEG-35 in addition to hindering the microsecond conformational dynamics shifts the intermediate state from 40 to 30 °C. Overall, our results provide new insight into deciphering the mechanism of crowder-induced changes in protein.
32569469	1	55	theme	crowders	294:301	arg1	presence					267:274	the presence	263:274	the presence of macromolecular crowders of various compositions, sizes, and shapes	263:344	One of the main differences in the intercellular environment compared to the laboratory condition is the presence of macromolecular crowders of various compositions, sizes, and shapes.
32569469	1	55	theme	crowders	294:301	arg1	differences					178:188	the main differences	169:188	the main differences in the intercellular environment compared to the laboratory condition	169:258	One of the main differences in the intercellular environment compared to the laboratory condition is the presence of macromolecular crowders of various compositions, sizes, and shapes.
32569469	1	55	theme	crowders	294:301	arg1	One					162:164	One	162:164	One	162:164	One of the main differences in the intercellular environment compared to the laboratory condition is the presence of macromolecular crowders of various compositions, sizes, and shapes.
32569469	9	56	theme	domain	1985:1990	arg1	dynamics					1973:1980	the microsecond conformational dynamics	1942:1980	the microsecond conformational dynamics of domain III of HSA in all states except the intermediate state	1942:2045	Rod-shaped dextran-40 and spherical ficoll-70 hinder the microsecond conformational dynamics of domain III of HSA in all states except the intermediate state.
32569469	9	57	theme	HSA	1999:2001	arg1	domain					1985:1990	domain III	1985:1994	domain III of HSA	1985:2001	Rod-shaped dextran-40 and spherical ficoll-70 hinder the microsecond conformational dynamics of domain III of HSA in all states except the intermediate state.
32569469	9	57	theme	HSA	1999:2001	arg1	HSA					1999:2001	HSA	1999:2001	HSA	1999:2001	Rod-shaped dextran-40 and spherical ficoll-70 hinder the microsecond conformational dynamics of domain III of HSA in all states except the intermediate state.
32569469	4	58	theme	destabilizing	1027:1039	arg1	effect					1051:1056	the destabilizing enthalpic effect	1023:1056	the destabilizing enthalpic effect	1023:1056	A complete thermodynamic analysis suggests that the stabilization by dextran-40 and ficoll-70 occurs mainly through stabilizing entropic effect, which is somewhat counteracted by the destabilizing enthalpic effect, in line with what is expected from the traditional interpretation of excluded volume and soft interaction.
32569469	8	59	theme	HSA	1779:1781	arg1	dynamics					1767:1774	The active-site dynamics	1751:1774	The active-site dynamics of HSA	1751:1781	The active-site dynamics of HSA are altered significantly in the presence of all the three differently shaped crowders used in the study.
32569469	1	60	theme	intercellular	197:209	arg1	environment					211:221	the intercellular environment	193:221	the intercellular environment compared to the laboratory condition	193:258	One of the main differences in the intercellular environment compared to the laboratory condition is the presence of macromolecular crowders of various compositions, sizes, and shapes.
32569469	4	61	theme	interaction	1153:1163	arg1	interpretation					1110:1123	the traditional interpretation	1094:1123	the traditional interpretation of excluded volume and soft interaction	1094:1163	A complete thermodynamic analysis suggests that the stabilization by dextran-40 and ficoll-70 occurs mainly through stabilizing entropic effect, which is somewhat counteracted by the destabilizing enthalpic effect, in line with what is expected from the traditional interpretation of excluded volume and soft interaction.
32569469	6	62	theme	associated	1398:1407	arg1	structure					1415:1423	the associated water structure	1394:1423	the associated water structure due to crowder-induced distortion	1394:1457	Our speculation is that the modulation of the associated water structure due to crowder-induced distortion plays a crucial role in modulating the entropic component.
32569469	7	63	theme	domain	1693:1698	arg1	profile					1682:1688	the thermal denaturation profile	1657:1688	the thermal denaturation profile of domain III of HSA	1657:1709	Moreover, while a two-state model can approximate the overall thermal denaturation of HSA in the absence and presence of various crowders, the thermal denaturation profile of domain III of HSA involves a distinct intermediate state.
32569469	4	64	theme	stabilizing	960:970	arg1	effect					981:986	stabilizing entropic effect	960:986	stabilizing entropic effect	960:986	A complete thermodynamic analysis suggests that the stabilization by dextran-40 and ficoll-70 occurs mainly through stabilizing entropic effect, which is somewhat counteracted by the destabilizing enthalpic effect, in line with what is expected from the traditional interpretation of excluded volume and soft interaction.
32569469	10	65	from	changes	2269:2275	arg1	protein					2280:2286	protein	2280:2286	protein	2280:2286	Mesh-like PEG-35 in addition to hindering the microsecond conformational dynamics shifts the intermediate state from 40 to 30 °C. Overall, our results provide new insight into deciphering the mechanism of crowder-induced changes in protein.
32569469	9	66	theme	spherical	1915:1923	arg1	ficoll-70					1925:1933	spherical ficoll-70	1915:1933	spherical ficoll-70	1915:1933	Rod-shaped dextran-40 and spherical ficoll-70 hinder the microsecond conformational dynamics of domain III of HSA in all states except the intermediate state.
32569469	2	67	theme	cell	725:728	arg1	environment					730:740	the cell environment	721:740	the cell environment	721:740	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	10	68	from	protein	2280:2286	arg1	mechanism					2240:2248	the mechanism	2236:2248	the mechanism of crowder-induced changes in protein	2236:2286	Mesh-like PEG-35 in addition to hindering the microsecond conformational dynamics shifts the intermediate state from 40 to 30 °C. Overall, our results provide new insight into deciphering the mechanism of crowder-induced changes in protein.
32569469	7	69	from	approximate	1556:1566	arg1	presence					1627:1634	presence	1627:1634	presence	1627:1634	Moreover, while a two-state model can approximate the overall thermal denaturation of HSA in the absence and presence of various crowders, the thermal denaturation profile of domain III of HSA involves a distinct intermediate state.
32569469	7	69	from	approximate	1556:1566	arg1	absence					1615:1621	absence	1615:1621	absence	1615:1621	Moreover, while a two-state model can approximate the overall thermal denaturation of HSA in the absence and presence of various crowders, the thermal denaturation profile of domain III of HSA involves a distinct intermediate state.
32569469	7	70	theme	thermal	1661:1667	arg1	profile					1682:1688	the thermal denaturation profile	1657:1688	the thermal denaturation profile of domain III of HSA	1657:1709	Moreover, while a two-state model can approximate the overall thermal denaturation of HSA in the absence and presence of various crowders, the thermal denaturation profile of domain III of HSA involves a distinct intermediate state.
32569469	2	71	theme	model	580:584	arg1	albumin					559:565	human serum albumin	547:565	human serum albumin (HSA)	547:571	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	2	71	theme	model	580:584	arg1	protein					586:592	the model protein	576:592	the model protein	576:592	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	10	72	dep	hindering	2080:2088	arg1	addition					2068:2075	addition	2068:2075	addition	2068:2075	Mesh-like PEG-35 in addition to hindering the microsecond conformational dynamics shifts the intermediate state from 40 to 30 °C. Overall, our results provide new insight into deciphering the mechanism of crowder-induced changes in protein.
32569469	5	73	theme	volume	1333:1338	arg1	prediction					1340:1349	the excluded volume prediction	1320:1349	the excluded volume prediction	1320:1349	Surprisingly, the destabilizing effect of PEG-35 is not through unfavorable interaction but through a destabilizing entropic effect, which is opposite to the excluded volume prediction.
32569469	10	74	theme	Mesh-like	2048:2056	arg1	PEG-35					2058:2063	Mesh-like PEG-35	2048:2063	Mesh-like PEG-35 in addition to hindering the microsecond conformational dynamics	2048:2128	Mesh-like PEG-35 in addition to hindering the microsecond conformational dynamics shifts the intermediate state from 40 to 30 °C. Overall, our results provide new insight into deciphering the mechanism of crowder-induced changes in protein.
32569469	7	75	theme	various	1639:1645	arg1	crowders					1647:1654	various crowders	1639:1654	various crowders	1639:1654	Moreover, while a two-state model can approximate the overall thermal denaturation of HSA in the absence and presence of various crowders, the thermal denaturation profile of domain III of HSA involves a distinct intermediate state.
32569469	6	76	theme	due	1425:1427	arg1	structure					1415:1423	the associated water structure	1394:1423	the associated water structure due to crowder-induced distortion	1394:1457	Our speculation is that the modulation of the associated water structure due to crowder-induced distortion plays a crucial role in modulating the entropic component.
32569469	0	77	theme	Protein	105:111	arg1	Unfolding					113:121	Protein Unfolding	105:121	Protein Unfolding	105:121	Shape-Dependent Macromolecular Crowding on the Thermodynamics and Microsecond Conformational Dynamics of Protein Unfolding Revealed at the Single-Molecule Level.
32569469	8	78	theme	shaped	1854:1859	arg1	crowders					1861:1868	all the three differently shaped crowders	1828:1868	all the three differently shaped crowders used in the study	1828:1886	The active-site dynamics of HSA are altered significantly in the presence of all the three differently shaped crowders used in the study.
32569469	1	79	theme	main	173:176	arg1	differences					178:188	the main differences	169:188	the main differences in the intercellular environment compared to the laboratory condition	169:258	One of the main differences in the intercellular environment compared to the laboratory condition is the presence of macromolecular crowders of various compositions, sizes, and shapes.
32569469	2	80	theme	unfolding	527:535	arg1	dynamics					507:514	microsecond conformational fluctuation dynamics	468:514	microsecond conformational fluctuation dynamics	468:514	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	2	80	theme	unfolding	527:535	arg1	thermodynamics					449:462	the thermodynamics	445:462	the thermodynamics	445:462	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	1	81	from	differences	178:188	arg1	environment					211:221	the intercellular environment	193:221	the intercellular environment compared to the laboratory condition	193:258	One of the main differences in the intercellular environment compared to the laboratory condition is the presence of macromolecular crowders of various compositions, sizes, and shapes.
32569469	10	82	theme	conformational	2106:2119	arg1	dynamics					2121:2128	the microsecond conformational dynamics	2090:2128	the microsecond conformational dynamics	2090:2128	Mesh-like PEG-35 in addition to hindering the microsecond conformational dynamics shifts the intermediate state from 40 to 30 °C. Overall, our results provide new insight into deciphering the mechanism of crowder-induced changes in protein.
32569469	6	83	theme	structure	1415:1423	arg1	modulation					1380:1389	the modulation	1376:1389	the modulation of the associated water structure due to crowder-induced distortion	1376:1457	Our speculation is that the modulation of the associated water structure due to crowder-induced distortion plays a crucial role in modulating the entropic component.
32569469	2	84	theme	serum	553:557	arg1	albumin					559:565	human serum albumin	547:565	human serum albumin (HSA)	547:571	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	2	84	theme	serum	553:557	arg1	crowders					682:689	macromolecular crowders	667:689	macromolecular crowders of different shapes	667:709	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	2	84	theme	serum	553:557	arg1	crowders					612:619	similar-sized crowders	598:619	similar-sized crowders	598:619	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	2	84	theme	serum	553:557	arg1	protein					586:592	the model protein	576:592	the model protein	576:592	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	2	84	theme	serum	553:557	arg1	HSA					568:570	HSA	568:570	HSA	568:570	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	0	85	theme	Macromolecular	16:29	arg1	Crowding					31:38	Shape-Dependent Macromolecular Crowding	0:38	Shape-Dependent Macromolecular Crowding on the Thermodynamics and Microsecond Conformational Dynamics of Protein Unfolding	0:121	Shape-Dependent Macromolecular Crowding on the Thermodynamics and Microsecond Conformational Dynamics of Protein Unfolding Revealed at the Single-Molecule Level.
32569469	2	86	theme	microsecond	468:478	arg1	dynamics					507:514	microsecond conformational fluctuation dynamics	468:514	microsecond conformational fluctuation dynamics	468:514	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	2	87	theme	different	694:702	arg1	shapes					704:709	different shapes	694:709	different shapes	694:709	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	2	88	theme	systematic	387:396	arg1	dependency					404:413	a systematic shape dependency	385:413	a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding	385:535	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	6	89	theme	entropic	1498:1505	arg1	component					1507:1515	the entropic component	1494:1515	the entropic component	1494:1515	Our speculation is that the modulation of the associated water structure due to crowder-induced distortion plays a crucial role in modulating the entropic component.
32569469	1	90	theme	compositions	314:325	arg1	crowders					294:301	macromolecular crowders	279:301	macromolecular crowders of various compositions, sizes, and shapes	279:344	One of the main differences in the intercellular environment compared to the laboratory condition is the presence of macromolecular crowders of various compositions, sizes, and shapes.
32569469	7	91	from	absence	1615:1621	arg1	approximate					1556:1566	approximate	1556:1566	approximate	1556:1566	Moreover, while a two-state model can approximate the overall thermal denaturation of HSA in the absence and presence of various crowders, the thermal denaturation profile of domain III of HSA involves a distinct intermediate state.
32569469	7	92	theme	intermediate	1731:1742	arg1	state					1744:1748	a distinct intermediate state	1720:1748	a distinct intermediate state	1720:1748	Moreover, while a two-state model can approximate the overall thermal denaturation of HSA in the absence and presence of various crowders, the thermal denaturation profile of domain III of HSA involves a distinct intermediate state.
32569469	1	93	theme	sizes	328:332	arg1	crowders					294:301	macromolecular crowders	279:301	macromolecular crowders of various compositions, sizes, and shapes	279:344	One of the main differences in the intercellular environment compared to the laboratory condition is the presence of macromolecular crowders of various compositions, sizes, and shapes.
32569469	7	94	from	presence	1627:1634	arg1	approximate					1556:1566	approximate	1556:1566	approximate	1556:1566	Moreover, while a two-state model can approximate the overall thermal denaturation of HSA in the absence and presence of various crowders, the thermal denaturation profile of domain III of HSA involves a distinct intermediate state.
32569469	10	95	theme	intermediate	2141:2152	arg1	state					2154:2158	the intermediate state	2137:2158	the intermediate state	2137:2158	Mesh-like PEG-35 in addition to hindering the microsecond conformational dynamics shifts the intermediate state from 40 to 30 °C. Overall, our results provide new insight into deciphering the mechanism of crowder-induced changes in protein.
32569469	9	96	theme	Rod-shaped	1889:1898	arg1	dextran-40					1900:1909	Rod-shaped dextran-40	1889:1909	Rod-shaped dextran-40	1889:1909	Rod-shaped dextran-40 and spherical ficoll-70 hinder the microsecond conformational dynamics of domain III of HSA in all states except the intermediate state.
32569469	2	97	theme	macromolecular	418:431	arg1	crowders					433:440	macromolecular crowders	418:440	macromolecular crowders	418:440	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	0	98	theme	Microsecond	66:76	arg1	Dynamics					93:100	Microsecond Conformational Dynamics	66:100	Microsecond Conformational Dynamics	66:100	Shape-Dependent Macromolecular Crowding on the Thermodynamics and Microsecond Conformational Dynamics of Protein Unfolding Revealed at the Single-Molecule Level.
32569469	5	99	theme	PEG-35	1208:1213	arg1	effect					1198:1203	the destabilizing effect	1180:1203	the destabilizing effect of PEG-35	1180:1213	Surprisingly, the destabilizing effect of PEG-35 is not through unfavorable interaction but through a destabilizing entropic effect, which is opposite to the excluded volume prediction.
32569469	7	100	theme	HSA	1604:1606	arg1	denaturation					1588:1599	the overall thermal denaturation	1568:1599	the overall thermal denaturation of HSA	1568:1606	Moreover, while a two-state model can approximate the overall thermal denaturation of HSA in the absence and presence of various crowders, the thermal denaturation profile of domain III of HSA involves a distinct intermediate state.
32569469	0	101	dep	Thermodynamics	47:60	arg1	the					43:45	the	43:45	the	43:45	Shape-Dependent Macromolecular Crowding on the Thermodynamics and Microsecond Conformational Dynamics of Protein Unfolding Revealed at the Single-Molecule Level.
32569469	2	102	theme	fluctuation	495:505	arg1	dynamics					507:514	microsecond conformational fluctuation dynamics	468:514	microsecond conformational fluctuation dynamics	468:514	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	4	103	theme	complete	846:853	arg1	analysis					869:876	A complete thermodynamic analysis	844:876	A complete thermodynamic analysis	844:876	A complete thermodynamic analysis suggests that the stabilization by dextran-40 and ficoll-70 occurs mainly through stabilizing entropic effect, which is somewhat counteracted by the destabilizing enthalpic effect, in line with what is expected from the traditional interpretation of excluded volume and soft interaction.
32569469	2	104	from	dependency	404:413	arg1	dynamics					507:514	microsecond conformational fluctuation dynamics	468:514	microsecond conformational fluctuation dynamics	468:514	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	2	104	from	dependency	404:413	arg1	thermodynamics					449:462	the thermodynamics	445:462	the thermodynamics	445:462	In this article, we have contemplated a systematic shape dependency of macromolecular crowders on the thermodynamics and microsecond conformational fluctuation dynamics of protein unfolding by taking human serum albumin (HSA) as the model protein and similar-sized crowders, namely, dextran-40, ficoll-70, and PEG-35 as macromolecular crowders of different shapes, to mimic the cell environment.
32569469	10	105	theme	crowder-induced	2253:2267	arg1	changes					2269:2275	crowder-induced changes	2253:2275	crowder-induced changes in protein	2253:2286	Mesh-like PEG-35 in addition to hindering the microsecond conformational dynamics shifts the intermediate state from 40 to 30 °C. Overall, our results provide new insight into deciphering the mechanism of crowder-induced changes in protein.
32569469	7	106	theme	overall	1572:1578	arg1	denaturation					1588:1599	the overall thermal denaturation	1568:1599	the overall thermal denaturation of HSA	1568:1606	Moreover, while a two-state model can approximate the overall thermal denaturation of HSA in the absence and presence of various crowders, the thermal denaturation profile of domain III of HSA involves a distinct intermediate state.
32569469	4	107	theme	volume	1137:1142	arg1	interpretation					1110:1123	the traditional interpretation	1094:1123	the traditional interpretation of excluded volume and soft interaction	1094:1163	A complete thermodynamic analysis suggests that the stabilization by dextran-40 and ficoll-70 occurs mainly through stabilizing entropic effect, which is somewhat counteracted by the destabilizing enthalpic effect, in line with what is expected from the traditional interpretation of excluded volume and soft interaction.
34755652	7	0	theme	opposite	1043:1050	arg1	trend					1052:1056	an opposite trend	1040:1056	an opposite trend	1040:1056	In the composition of stalks, the fraction of hemicellulose was the highest, followed by that of cellulose, whereas in the composition of leaves and whole plant samples, an opposite trend was observed.
34755652	1	1	theme	kinetic	172:178	arg1	parameters					180:189	the kinetic parameters	168:189	the kinetic parameters of biomass based on thermogravimetric analysis and the Coats-Redfern procedure with 27 model equations	168:292	This article presents a methodology for determining the kinetic parameters of biomass based on thermogravimetric analysis and the Coats-Redfern procedure with 27 model equations.
34755652	1	2	theme	model	278:282	arg1	equations					284:292	27 model equations	275:292	27 model equations	275:292	This article presents a methodology for determining the kinetic parameters of biomass based on thermogravimetric analysis and the Coats-Redfern procedure with 27 model equations.
34755652	5	3	theme	fraction	721:728	arg1	parts					702:706	selected parts	693:706	selected parts of the stalk fraction	693:728	The activation energy reached up to 71.6 kJ/mol for tżhe selected parts of the stalk fraction, whereas it decreased to 6.5 kJ/mol for the others.
34755652	7	4	located	observed	1062:1069	arg1	composition					993:1003	the composition	989:1003	the composition of leaves and whole plant samples	989:1037	In the composition of stalks, the fraction of hemicellulose was the highest, followed by that of cellulose, whereas in the composition of leaves and whole plant samples, an opposite trend was observed.
34755652	7	4	located	observed	1062:1069	arg2	trend					1052:1056	an opposite trend	1040:1056	an opposite trend	1040:1056	In the composition of stalks, the fraction of hemicellulose was the highest, followed by that of cellulose, whereas in the composition of leaves and whole plant samples, an opposite trend was observed.
34755652	4	5	theme	reaction	597:604	arg1	growth					617:622	the first-order reaction and nuclei growth	581:622	the first-order reaction and nuclei growth of n = 1.5	581:633	The last sub-step showed good convergence with the first-order reaction and nuclei growth of n = 1.5.
34755652	0	6	theme	semi-statistical	90:105	arg1	methods					107:113	semi-statistical methods	90:113	semi-statistical methods	90:113	Analyzing the kinetics of waste plant biomass pyrolysis via thermogravimetry modeling and semi-statistical methods.
34755652	4	7	theme	first-order	585:595	arg1	reaction					597:604	first-order reaction	585:604	first-order reaction	585:604	The last sub-step showed good convergence with the first-order reaction and nuclei growth of n = 1.5.
34755652	3	8	theme	first	365:369	arg1	sub-stage					371:379	The first sub-stage	361:379	The first sub-stage of pyrolysis	361:392	The first sub-stage of pyrolysis was a first-order reaction with nuclei growth of n = 1, and the second sub-stage indicated a different kinetic order (1.5) of the reaction.
34755652	3	8	theme	first	365:369	arg1	reaction					412:419	a first-order reaction	398:419	a first-order reaction with nuclei growth of n = 1	398:447	The first sub-stage of pyrolysis was a first-order reaction with nuclei growth of n = 1, and the second sub-stage indicated a different kinetic order (1.5) of the reaction.
34755652	4	9	theme	n = 1.5	627:633	arg1	growth					617:622	the first-order reaction and nuclei growth	581:622	the first-order reaction and nuclei growth of n = 1.5	581:633	The last sub-step showed good convergence with the first-order reaction and nuclei growth of n = 1.5.
34755652	3	10	theme	reaction	524:531	arg1	order					505:509	a different kinetic order (1.5)	485:515	a different kinetic order (1.5) of the reaction	485:531	The first sub-stage of pyrolysis was a first-order reaction with nuclei growth of n = 1, and the second sub-stage indicated a different kinetic order (1.5) of the reaction.
34755652	1	11	theme	biomass	194:200	arg1	parameters					180:189	the kinetic parameters	168:189	the kinetic parameters of biomass based on thermogravimetric analysis and the Coats-Redfern procedure with 27 model equations	168:292	This article presents a methodology for determining the kinetic parameters of biomass based on thermogravimetric analysis and the Coats-Redfern procedure with 27 model equations.
34755652	0	12	theme	biomass	38:44	arg1	pyrolysis					46:54	waste plant biomass pyrolysis	26:54	waste plant biomass pyrolysis	26:54	Analyzing the kinetics of waste plant biomass pyrolysis via thermogravimetry modeling and semi-statistical methods.
34755652	2	13	used	used	348:351	arg2	samples					301:307	Maize samples	295:307	Maize samples stored for approximately one year	295:341	Maize samples stored for approximately one year were used herein.
34755652	2	14	theme	Maize	295:299	arg1	samples					301:307	Maize samples	295:307	Maize samples stored for approximately one year	295:341	Maize samples stored for approximately one year were used herein.
34755652	3	15	theme	first-order	400:410	arg1	sub-stage					371:379	The first sub-stage	361:379	The first sub-stage of pyrolysis	361:392	The first sub-stage of pyrolysis was a first-order reaction with nuclei growth of n = 1, and the second sub-stage indicated a different kinetic order (1.5) of the reaction.
34755652	3	15	theme	first-order	400:410	arg1	reaction					412:419	a first-order reaction	398:419	a first-order reaction with nuclei growth of n = 1	398:447	The first sub-stage of pyrolysis was a first-order reaction with nuclei growth of n = 1, and the second sub-stage indicated a different kinetic order (1.5) of the reaction.
34755652	3	16	theme	n = 1	443:447	arg1	growth					433:438	nuclei growth	426:438	nuclei growth of n = 1	426:447	The first sub-stage of pyrolysis was a first-order reaction with nuclei growth of n = 1, and the second sub-stage indicated a different kinetic order (1.5) of the reaction.
34755652	5	17	theme	activation	640:649	arg1	energy					651:656	The activation energy	636:656	The activation energy	636:656	The activation energy reached up to 71.6 kJ/mol for tżhe selected parts of the stalk fraction, whereas it decreased to 6.5 kJ/mol for the others.
34755652	1	18	with	analysis	229:236	arg1	equations					284:292	27 model equations	275:292	27 model equations	275:292	This article presents a methodology for determining the kinetic parameters of biomass based on thermogravimetric analysis and the Coats-Redfern procedure with 27 model equations.
34755652	6	19	theme	simplified	784:793	arg1	method					795:800	A simplified method	782:800	A simplified method for approximating the composition of the biomass	782:849	A simplified method for approximating the composition of the biomass is also presented.
34755652	3	20	with	reaction	412:419	arg1	growth					433:438	nuclei growth	426:438	nuclei growth of n = 1	426:447	The first sub-stage of pyrolysis was a first-order reaction with nuclei growth of n = 1, and the second sub-stage indicated a different kinetic order (1.5) of the reaction.
34755652	1	21	theme	thermogravimetric	211:227	arg1	analysis					229:236	thermogravimetric analysis	211:236	thermogravimetric analysis	211:236	This article presents a methodology for determining the kinetic parameters of biomass based on thermogravimetric analysis and the Coats-Redfern procedure with 27 model equations.
34755652	0	22	theme	plant	32:36	arg1	pyrolysis					46:54	waste plant biomass pyrolysis	26:54	waste plant biomass pyrolysis	26:54	Analyzing the kinetics of waste plant biomass pyrolysis via thermogravimetry modeling and semi-statistical methods.
34755652	4	23	theme	good	559:562	arg1	convergence					564:574	good convergence	559:574	good convergence	559:574	The last sub-step showed good convergence with the first-order reaction and nuclei growth of n = 1.5.
34755652	7	24	theme	whole	1019:1023	arg1	samples					1031:1037	whole plant samples	1019:1037	whole plant samples	1019:1037	In the composition of stalks, the fraction of hemicellulose was the highest, followed by that of cellulose, whereas in the composition of leaves and whole plant samples, an opposite trend was observed.
34755652	3	25	theme	second	458:463	arg1	sub-stage					465:473	the second sub-stage	454:473	the second sub-stage	454:473	The first sub-stage of pyrolysis was a first-order reaction with nuclei growth of n = 1, and the second sub-stage indicated a different kinetic order (1.5) of the reaction.
34755652	0	26	theme	waste	26:30	arg1	pyrolysis					46:54	waste plant biomass pyrolysis	26:54	waste plant biomass pyrolysis	26:54	Analyzing the kinetics of waste plant biomass pyrolysis via thermogravimetry modeling and semi-statistical methods.
34755652	5	27	theme	stalk	715:719	arg1	fraction					721:728	the stalk fraction	711:728	the stalk fraction	711:728	The activation energy reached up to 71.6 kJ/mol for tżhe selected parts of the stalk fraction, whereas it decreased to 6.5 kJ/mol for the others.
34755652	0	28	theme	pyrolysis	46:54	arg1	kinetics					14:21	the kinetics	10:21	the kinetics of waste plant biomass pyrolysis	10:54	Analyzing the kinetics of waste plant biomass pyrolysis via thermogravimetry modeling and semi-statistical methods.
34755652	7	29	theme	leaves	1008:1013	arg1	composition					993:1003	the composition	989:1003	the composition of leaves and whole plant samples	989:1037	In the composition of stalks, the fraction of hemicellulose was the highest, followed by that of cellulose, whereas in the composition of leaves and whole plant samples, an opposite trend was observed.
34755652	6	30	theme	biomass	843:849	arg1	composition					824:834	the composition	820:834	the composition of the biomass	820:849	A simplified method for approximating the composition of the biomass is also presented.
34755652	7	31	theme	plant	1025:1029	arg1	samples					1031:1037	whole plant samples	1019:1037	whole plant samples	1019:1037	In the composition of stalks, the fraction of hemicellulose was the highest, followed by that of cellulose, whereas in the composition of leaves and whole plant samples, an opposite trend was observed.
34755652	3	32	theme	nuclei	426:431	arg1	growth					433:438	nuclei growth	426:438	nuclei growth of n = 1	426:447	The first sub-stage of pyrolysis was a first-order reaction with nuclei growth of n = 1, and the second sub-stage indicated a different kinetic order (1.5) of the reaction.
34755652	4	33	theme	last	538:541	arg1	sub-step					543:550	The last sub-step	534:550	The last sub-step	534:550	The last sub-step showed good convergence with the first-order reaction and nuclei growth of n = 1.5.
34755652	7	34	theme	samples	1031:1037	arg1	composition					993:1003	the composition	989:1003	the composition of leaves and whole plant samples	989:1037	In the composition of stalks, the fraction of hemicellulose was the highest, followed by that of cellulose, whereas in the composition of leaves and whole plant samples, an opposite trend was observed.
34755652	7	35	theme	hemicellulose	916:928	arg1	highest					938:944	highest	938:944	highest	938:944	In the composition of stalks, the fraction of hemicellulose was the highest, followed by that of cellulose, whereas in the composition of leaves and whole plant samples, an opposite trend was observed.
34755652	7	35	theme	hemicellulose	916:928	arg1	fraction					904:911	the fraction	900:911	the fraction of hemicellulose	900:928	In the composition of stalks, the fraction of hemicellulose was the highest, followed by that of cellulose, whereas in the composition of leaves and whole plant samples, an opposite trend was observed.
34755652	3	36	theme	different	487:495	arg1	order					505:509	a different kinetic order (1.5)	485:515	a different kinetic order (1.5) of the reaction	485:531	The first sub-stage of pyrolysis was a first-order reaction with nuclei growth of n = 1, and the second sub-stage indicated a different kinetic order (1.5) of the reaction.
34755652	7	37	dep	highest	938:944	arg1	whereas					978:984	whereas	978:984	whereas	978:984	In the composition of stalks, the fraction of hemicellulose was the highest, followed by that of cellulose, whereas in the composition of leaves and whole plant samples, an opposite trend was observed.
34755652	4	38	theme	nuclei	610:615	arg1	growth					617:622	the first-order reaction and nuclei growth	581:622	the first-order reaction and nuclei growth of n = 1.5	581:633	The last sub-step showed good convergence with the first-order reaction and nuclei growth of n = 1.5.
34755652	5	39	theme	selected	693:700	arg1	parts					702:706	selected parts	693:706	selected parts of the stalk fraction	693:728	The activation energy reached up to 71.6 kJ/mol for tżhe selected parts of the stalk fraction, whereas it decreased to 6.5 kJ/mol for the others.
34755652	1	40	with	procedure	260:268	arg1	equations					284:292	27 model equations	275:292	27 model equations	275:292	This article presents a methodology for determining the kinetic parameters of biomass based on thermogravimetric analysis and the Coats-Redfern procedure with 27 model equations.
34755652	3	41	theme	kinetic	497:503	arg1	order					505:509	a different kinetic order (1.5)	485:515	a different kinetic order (1.5) of the reaction	485:531	The first sub-stage of pyrolysis was a first-order reaction with nuclei growth of n = 1, and the second sub-stage indicated a different kinetic order (1.5) of the reaction.
34755652	1	42	theme	Coats-Redfern	246:258	arg1	procedure					260:268	the Coats-Redfern procedure	242:268	the Coats-Redfern procedure	242:268	This article presents a methodology for determining the kinetic parameters of biomass based on thermogravimetric analysis and the Coats-Redfern procedure with 27 model equations.
34755652	0	43	theme	thermogravimetry	60:75	arg1	modeling					77:84	thermogravimetry modeling	60:84	thermogravimetry modeling	60:84	Analyzing the kinetics of waste plant biomass pyrolysis via thermogravimetry modeling and semi-statistical methods.
34755652	7	44	theme	stalks	892:897	arg1	composition					877:887	the composition	873:887	the composition of stalks	873:897	In the composition of stalks, the fraction of hemicellulose was the highest, followed by that of cellulose, whereas in the composition of leaves and whole plant samples, an opposite trend was observed.
34755652	3	45	theme	pyrolysis	384:392	arg1	sub-stage					371:379	The first sub-stage	361:379	The first sub-stage of pyrolysis	361:392	The first sub-stage of pyrolysis was a first-order reaction with nuclei growth of n = 1, and the second sub-stage indicated a different kinetic order (1.5) of the reaction.
34755652	3	45	theme	pyrolysis	384:392	arg1	reaction					412:419	a first-order reaction	398:419	a first-order reaction with nuclei growth of n = 1	398:447	The first sub-stage of pyrolysis was a first-order reaction with nuclei growth of n = 1, and the second sub-stage indicated a different kinetic order (1.5) of the reaction.
33038400	6	0	theme	beeswax	1015:1021	arg1	coating					1023:1029	beeswax coating	1015:1029	beeswax coating	1015:1029	Results indicated that a starch foam/5 wt% WH composite with beeswax coating was a biodegradable foam that could possibly replace commercial non-degradable foam.
33038400	3	1	theme	highest	535:541	arg1	load					517:520	maximum load	509:520	maximum load (3.52%)	509:528	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	1	theme	highest	535:541	arg1	232.00 MPa					552:561	232.00 MPa	552:561	232.00 MPa	552:561	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	1	theme	highest	535:541	arg1	modulus					543:549	the highest modulus	531:549	the highest modulus (232.00 MPa)	531:562	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	2	theme	uniform	614:620	arg1	0.44 ± 0.09 mm					646:659	0.44 ± 0.09 mm	646:659	0.44 ± 0.09 mm	646:659	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	2	theme	uniform	614:620	arg1	distribution					632:643	the most uniform cell size distribution	605:643	the most uniform cell size distribution (0.44 ± 0.09 mm)	605:660	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	0	3	theme	beeswax	62:68	arg1	coating					70:76	beeswax coating	62:76	beeswax coating for food packaging applications	62:108	Novel composite foam made from starch and water hyacinth with beeswax coating for food packaging applications.
33038400	5	4	theme	starch	831:836	arg1	foams					838:842	the starch foams	827:842	the starch foams retained their shape after immersion in distilled water and their water solubility	827:925	After being coated with beeswax, the starch foams retained their shape after immersion in distilled water and their water solubility was significantly reduced.
33038400	3	5	theme	5 wt	384:387	arg1	powder					393:398	5 wt% WH powder	384:398	5 wt% WH powder	384:398	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	1	6	theme	hot	213:215	arg1	mold					217:220	a hot mold	211:220	a hot mold	211:220	A novel composite foam was prepared from native cassava starch and water hyacinth (WH) by baking in a hot mold.
33038400	0	7	theme	food	82:85	arg1	applications					97:108	food packaging applications	82:108	food packaging applications	82:108	Novel composite foam made from starch and water hyacinth with beeswax coating for food packaging applications.
33038400	6	8	theme	starch	979:984	arg1	foam					1051:1054	a biodegradable foam	1035:1054	a biodegradable foam that could possibly replace commercial non-degradable foam	1035:1113	Results indicated that a starch foam/5 wt% WH composite with beeswax coating was a biodegradable foam that could possibly replace commercial non-degradable foam.
33038400	6	8	theme	starch	979:984	arg1	composite					1000:1008	a starch foam/5 wt% WH composite	977:1008	a starch foam/5 wt% WH composite with beeswax coating	977:1029	Results indicated that a starch foam/5 wt% WH composite with beeswax coating was a biodegradable foam that could possibly replace commercial non-degradable foam.
33038400	2	9	theme	WH	238:239	arg1	content					248:254	WH powder content	238:254	WH powder content (0, 3, 5, 7 or 10 wt%, dry starch basis)	238:295	The effects of WH powder content (0, 3, 5, 7 or 10 wt%, dry starch basis) on the properties of the starch foam were investigated.
33038400	4	10	theme	same	752:755	arg1	properties					757:766	the same properties	748:766	the same properties of some commercial foams	748:791	Moreover, mechanical properties of starch foam with 5 wt% WH powder were better than the same properties of some commercial foams.
33038400	4	11	theme	WH	721:722	arg1	powder					724:729	5 wt% WH powder	715:729	5 wt% WH powder	715:729	Moreover, mechanical properties of starch foam with 5 wt% WH powder were better than the same properties of some commercial foams.
33038400	6	12	theme	biodegradable	1037:1049	arg1	foam					1051:1054	a biodegradable foam	1035:1054	a biodegradable foam that could possibly replace commercial non-degradable foam	1035:1113	Results indicated that a starch foam/5 wt% WH composite with beeswax coating was a biodegradable foam that could possibly replace commercial non-degradable foam.
33038400	6	12	theme	biodegradable	1037:1049	arg1	composite					1000:1008	a starch foam/5 wt% WH composite	977:1008	a starch foam/5 wt% WH composite with beeswax coating	977:1029	Results indicated that a starch foam/5 wt% WH composite with beeswax coating was a biodegradable foam that could possibly replace commercial non-degradable foam.
33038400	3	13	theme	highest	414:420	arg1	stress					431:436	the highest flexural stress	410:436	the highest flexural stress at maximum load (3.42 MPa)	410:463	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	13	theme	highest	414:420	arg1	strain					487:492	the highest flexural strain	466:492	the highest flexural strain (extension)	466:504	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	14	theme	starch	355:360	arg1	formulation					367:377	A starch foam formulation	353:377	A starch foam formulation with 5 wt% WH powder	353:398	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	15	theme	foam	362:365	arg1	formulation					367:377	A starch foam formulation	353:377	A starch foam formulation with 5 wt% WH powder	353:398	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	6	16	theme	commercial	1084:1093	arg1	foam					1110:1113	commercial non-degradable foam	1084:1113	commercial non-degradable foam	1084:1113	Results indicated that a starch foam/5 wt% WH composite with beeswax coating was a biodegradable foam that could possibly replace commercial non-degradable foam.
33038400	1	17	theme	native	152:157	arg1	starch					167:172	native cassava starch	152:172	native cassava starch	152:172	A novel composite foam was prepared from native cassava starch and water hyacinth (WH) by baking in a hot mold.
33038400	0	18	theme	packaging	87:95	arg1	applications					97:108	food packaging applications	82:108	food packaging applications	82:108	Novel composite foam made from starch and water hyacinth with beeswax coating for food packaging applications.
33038400	1	19	theme	cassava	159:165	arg1	starch					167:172	native cassava starch	152:172	native cassava starch	152:172	A novel composite foam was prepared from native cassava starch and water hyacinth (WH) by baking in a hot mold.
33038400	0	20	theme	composite	6:14	arg1	foam					16:19	Novel composite foam	0:19	Novel composite foam	0:19	Novel composite foam made from starch and water hyacinth with beeswax coating for food packaging applications.
33038400	3	21	theme	WH	390:391	arg1	powder					393:398	5 wt% WH powder	384:398	5 wt% WH powder	384:398	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	4	22	with	foam	705:708	arg1	powder					724:729	5 wt% WH powder	715:729	5 wt% WH powder	715:729	Moreover, mechanical properties of starch foam with 5 wt% WH powder were better than the same properties of some commercial foams.
33038400	0	23	theme	Novel	0:4	arg1	foam					16:19	Novel composite foam	0:19	Novel composite foam	0:19	Novel composite foam made from starch and water hyacinth with beeswax coating for food packaging applications.
33038400	3	24	with	formulation	367:377	arg1	powder					393:398	5 wt% WH powder	384:398	5 wt% WH powder	384:398	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	25	theme	maximum	441:447	arg1	3.42 MPa					455:462	3.42 MPa	455:462	3.42 MPa	455:462	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	25	theme	maximum	441:447	arg1	load					449:452	maximum load	441:452	maximum load (3.42 MPa)	441:463	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	26	from	load	449:452	arg1	stress					431:436	the highest flexural stress	410:436	the highest flexural stress at maximum load (3.42 MPa)	410:463	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	26	from	load	449:452	arg1	strain					487:492	the highest flexural strain	466:492	the highest flexural strain (extension)	466:504	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	2	27	theme	content	248:254	arg1	effects					227:233	The effects	223:233	The effects of WH powder content (0, 3, 5, 7 or 10 wt%, dry starch basis) on the properties of the starch foam	223:332	The effects of WH powder content (0, 3, 5, 7 or 10 wt%, dry starch basis) on the properties of the starch foam were investigated.
33038400	5	28	from	immersion	871:879	arg1	water					894:898	distilled water	884:898	distilled water	884:898	After being coated with beeswax, the starch foams retained their shape after immersion in distilled water and their water solubility was significantly reduced.
33038400	5	28	from	immersion	871:879	arg1	solubility					916:925	their water solubility	904:925	their water solubility	904:925	After being coated with beeswax, the starch foams retained their shape after immersion in distilled water and their water solubility was significantly reduced.
33038400	4	29	theme	foam	705:708	arg1	properties					684:693	mechanical properties	673:693	mechanical properties of starch foam with 5 wt% WH powder	673:729	Moreover, mechanical properties of starch foam with 5 wt% WH powder were better than the same properties of some commercial foams.
33038400	4	30	theme	5 wt	715:718	arg1	powder					724:729	5 wt% WH powder	715:729	5 wt% WH powder	715:729	Moreover, mechanical properties of starch foam with 5 wt% WH powder were better than the same properties of some commercial foams.
33038400	2	31	theme	powder	241:246	arg1	content					248:254	WH powder content	238:254	WH powder content (0, 3, 5, 7 or 10 wt%, dry starch basis)	238:295	The effects of WH powder content (0, 3, 5, 7 or 10 wt%, dry starch basis) on the properties of the starch foam were investigated.
33038400	4	32	theme	%	719:719	arg1	powder					724:729	5 wt% WH powder	715:729	5 wt% WH powder	715:729	Moreover, mechanical properties of starch foam with 5 wt% WH powder were better than the same properties of some commercial foams.
33038400	2	33	theme	starch	283:288	arg1	%					276:276	0, 3, 5, 7 or 10 wt%	257:276	0, 3, 5, 7 or 10 wt%	257:276	The effects of WH powder content (0, 3, 5, 7 or 10 wt%, dry starch basis) on the properties of the starch foam were investigated.
33038400	2	33	theme	starch	283:288	arg1	basis					290:294	dry starch basis	279:294	dry starch basis	279:294	The effects of WH powder content (0, 3, 5, 7 or 10 wt%, dry starch basis) on the properties of the starch foam were investigated.
33038400	4	34	theme	commercial	776:785	arg1	foams					787:791	some commercial foams	771:791	some commercial foams	771:791	Moreover, mechanical properties of starch foam with 5 wt% WH powder were better than the same properties of some commercial foams.
33038400	3	35	theme	cell	622:625	arg1	0.44 ± 0.09 mm					646:659	0.44 ± 0.09 mm	646:659	0.44 ± 0.09 mm	646:659	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	35	theme	cell	622:625	arg1	distribution					632:643	the most uniform cell size distribution	605:643	the most uniform cell size distribution (0.44 ± 0.09 mm)	605:660	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	4	36	theme	foams	787:791	arg1	properties					757:766	the same properties	748:766	the same properties of some commercial foams	748:791	Moreover, mechanical properties of starch foam with 5 wt% WH powder were better than the same properties of some commercial foams.
33038400	5	37	theme	water	910:914	arg1	solubility					916:925	their water solubility	904:925	their water solubility	904:925	After being coated with beeswax, the starch foams retained their shape after immersion in distilled water and their water solubility was significantly reduced.
33038400	6	38	theme	non-degradable	1095:1108	arg1	foam					1110:1113	commercial non-degradable foam	1084:1113	commercial non-degradable foam	1084:1113	Results indicated that a starch foam/5 wt% WH composite with beeswax coating was a biodegradable foam that could possibly replace commercial non-degradable foam.
33038400	6	39	theme	WH	997:998	arg1	foam					1051:1054	a biodegradable foam	1035:1054	a biodegradable foam that could possibly replace commercial non-degradable foam	1035:1113	Results indicated that a starch foam/5 wt% WH composite with beeswax coating was a biodegradable foam that could possibly replace commercial non-degradable foam.
33038400	6	39	theme	WH	997:998	arg1	composite					1000:1008	a starch foam/5 wt% WH composite	977:1008	a starch foam/5 wt% WH composite with beeswax coating	977:1029	Results indicated that a starch foam/5 wt% WH composite with beeswax coating was a biodegradable foam that could possibly replace commercial non-degradable foam.
33038400	2	40	dep	content	248:254	arg1	%					276:276	0, 3, 5, 7 or 10 wt%	257:276	0, 3, 5, 7 or 10 wt%	257:276	The effects of WH powder content (0, 3, 5, 7 or 10 wt%, dry starch basis) on the properties of the starch foam were investigated.
33038400	2	40	dep	content	248:254	arg1	basis					290:294	dry starch basis	279:294	dry starch basis	279:294	The effects of WH powder content (0, 3, 5, 7 or 10 wt%, dry starch basis) on the properties of the starch foam were investigated.
33038400	2	41	theme	foam	329:332	arg1	properties					304:313	the properties	300:313	the properties of the starch foam	300:332	The effects of WH powder content (0, 3, 5, 7 or 10 wt%, dry starch basis) on the properties of the starch foam were investigated.
33038400	3	42	theme	lowest	569:574	arg1	content					585:591	the lowest moisture content	565:591	the lowest moisture content (6.77%)	565:599	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	42	theme	lowest	569:574	arg1	%					598:598	6.77%	594:598	6.77%	594:598	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	4	43	theme	mechanical	673:682	arg1	properties					684:693	mechanical properties	673:693	mechanical properties of starch foam with 5 wt% WH powder	673:729	Moreover, mechanical properties of starch foam with 5 wt% WH powder were better than the same properties of some commercial foams.
33038400	6	44	theme	%	995:995	arg1	foam					1051:1054	a biodegradable foam	1035:1054	a biodegradable foam that could possibly replace commercial non-degradable foam	1035:1113	Results indicated that a starch foam/5 wt% WH composite with beeswax coating was a biodegradable foam that could possibly replace commercial non-degradable foam.
33038400	6	44	theme	%	995:995	arg1	composite					1000:1008	a starch foam/5 wt% WH composite	977:1008	a starch foam/5 wt% WH composite with beeswax coating	977:1029	Results indicated that a starch foam/5 wt% WH composite with beeswax coating was a biodegradable foam that could possibly replace commercial non-degradable foam.
33038400	2	45	from	effects	227:233	arg1	properties					304:313	the properties	300:313	the properties of the starch foam	300:332	The effects of WH powder content (0, 3, 5, 7 or 10 wt%, dry starch basis) on the properties of the starch foam were investigated.
33038400	3	46	theme	moisture	576:583	arg1	content					585:591	the lowest moisture content	565:591	the lowest moisture content (6.77%)	565:599	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	46	theme	moisture	576:583	arg1	%					598:598	6.77%	594:598	6.77%	594:598	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	4	47	theme	starch	698:703	arg1	foam					705:708	starch foam	698:708	starch foam with 5 wt% WH powder	698:729	Moreover, mechanical properties of starch foam with 5 wt% WH powder were better than the same properties of some commercial foams.
33038400	6	48	theme	foam/5 wt	986:994	arg1	foam					1051:1054	a biodegradable foam	1035:1054	a biodegradable foam that could possibly replace commercial non-degradable foam	1035:1113	Results indicated that a starch foam/5 wt% WH composite with beeswax coating was a biodegradable foam that could possibly replace commercial non-degradable foam.
33038400	6	48	theme	foam/5 wt	986:994	arg1	composite					1000:1008	a starch foam/5 wt% WH composite	977:1008	a starch foam/5 wt% WH composite with beeswax coating	977:1029	Results indicated that a starch foam/5 wt% WH composite with beeswax coating was a biodegradable foam that could possibly replace commercial non-degradable foam.
33038400	3	49	theme	highest	470:476	arg1	extension					495:503	extension	495:503	extension	495:503	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	49	theme	highest	470:476	arg1	stress					431:436	the highest flexural stress	410:436	the highest flexural stress at maximum load (3.42 MPa)	410:463	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	49	theme	highest	470:476	arg1	strain					487:492	the highest flexural strain	466:492	the highest flexural strain (extension)	466:504	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	1	50	theme	novel	113:117	arg1	foam					129:132	A novel composite foam	111:132	A novel composite foam	111:132	A novel composite foam was prepared from native cassava starch and water hyacinth (WH) by baking in a hot mold.
33038400	3	51	theme	flexural	422:429	arg1	stress					431:436	the highest flexural stress	410:436	the highest flexural stress at maximum load (3.42 MPa)	410:463	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	51	theme	flexural	422:429	arg1	strain					487:492	the highest flexural strain	466:492	the highest flexural strain (extension)	466:504	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	52	theme	flexural	478:485	arg1	extension					495:503	extension	495:503	extension	495:503	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	52	theme	flexural	478:485	arg1	stress					431:436	the highest flexural stress	410:436	the highest flexural stress at maximum load (3.42 MPa)	410:463	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	52	theme	flexural	478:485	arg1	strain					487:492	the highest flexural strain	466:492	the highest flexural strain (extension)	466:504	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	0	53	with	water	42:46	arg1	coating					70:76	beeswax coating	62:76	beeswax coating for food packaging applications	62:108	Novel composite foam made from starch and water hyacinth with beeswax coating for food packaging applications.
33038400	1	54	theme	composite	119:127	arg1	foam					129:132	A novel composite foam	111:132	A novel composite foam	111:132	A novel composite foam was prepared from native cassava starch and water hyacinth (WH) by baking in a hot mold.
33038400	3	55	theme	size	627:630	arg1	0.44 ± 0.09 mm					646:659	0.44 ± 0.09 mm	646:659	0.44 ± 0.09 mm	646:659	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	55	theme	size	627:630	arg1	distribution					632:643	the most uniform cell size distribution	605:643	the most uniform cell size distribution (0.44 ± 0.09 mm)	605:660	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	56	theme	maximum	509:515	arg1	modulus					543:549	the highest modulus	531:549	the highest modulus (232.00 MPa)	531:562	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	56	theme	maximum	509:515	arg1	load					517:520	maximum load	509:520	maximum load (3.52%)	509:528	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	56	theme	maximum	509:515	arg1	%					527:527	3.52%	523:527	3.52%	523:527	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	3	57	theme	%	388:388	arg1	powder					393:398	5 wt% WH powder	384:398	5 wt% WH powder	384:398	A starch foam formulation with 5 wt% WH powder exhibited the highest flexural stress at maximum load (3.42 MPa), the highest flexural strain (extension) at maximum load (3.52%), the highest modulus (232.00 MPa), the lowest moisture content (6.77%) and the most uniform cell size distribution (0.44 ± 0.09 mm).
33038400	2	58	theme	dry	279:281	arg1	%					276:276	0, 3, 5, 7 or 10 wt%	257:276	0, 3, 5, 7 or 10 wt%	257:276	The effects of WH powder content (0, 3, 5, 7 or 10 wt%, dry starch basis) on the properties of the starch foam were investigated.
33038400	2	58	theme	dry	279:281	arg1	basis					290:294	dry starch basis	279:294	dry starch basis	279:294	The effects of WH powder content (0, 3, 5, 7 or 10 wt%, dry starch basis) on the properties of the starch foam were investigated.
33038400	5	59	theme	distilled	884:892	arg1	water					894:898	distilled water	884:898	distilled water	884:898	After being coated with beeswax, the starch foams retained their shape after immersion in distilled water and their water solubility was significantly reduced.
33038400	0	60	with	starch	31:36	arg1	coating					70:76	beeswax coating	62:76	beeswax coating for food packaging applications	62:108	Novel composite foam made from starch and water hyacinth with beeswax coating for food packaging applications.
33038400	2	61	theme	starch	322:327	arg1	foam					329:332	the starch foam	318:332	the starch foam	318:332	The effects of WH powder content (0, 3, 5, 7 or 10 wt%, dry starch basis) on the properties of the starch foam were investigated.
33038400	6	62	with	composite	1000:1008	arg1	coating					1023:1029	beeswax coating	1015:1029	beeswax coating	1015:1029	Results indicated that a starch foam/5 wt% WH composite with beeswax coating was a biodegradable foam that could possibly replace commercial non-degradable foam.
34680528	5	0	theme	pathogenic	840:849	arg1	activity					851:858	The pathogenic activity	836:858	The pathogenic activity of serum IgG	836:871	The pathogenic activity of serum IgG was measured in vitro on immortalized keratinocytes, by immunofluorescence and dissociation assays, and IgG N-glycans were analyzed by mass spectrometry.
34680528	0	1	from	Properties	24:33	arg1	Patients					82:89	Patients	82:89	Patients with Pemphigus after Corticosteroid Treatment	82:135	Longitudinal Pathogenic Properties and N-Glycosylation Profile of Antibodies from Patients with Pemphigus after Corticosteroid Treatment.
34680528	4	2	theme	profile	770:776	arg1	pathogenicity					707:719	the pathogenicity	703:719	the pathogenicity of pemphigus serum IgG and their N-glycosylation profile	703:776	The purpose of this study was to assess, longitudinally, the pathogenicity of pemphigus serum IgG and their N-glycosylation profile during phases of disease activity and clinical remission.
34680528	3	3	theme	IgG	580:582	arg1	activity					558:565	the pathogenic activity	543:565	the pathogenic activity of pemphigus IgG	543:582	Since modifications of IgG N-glycosylation have been described in some autoimmune diseases, we hypothesized that changes in the pathogenic activity of pemphigus IgG could be related to changes in their N-glycosylation profile.
34680528	3	4	from	changes	604:610	arg1	profile					637:643	their N-glycosylation profile	615:643	their N-glycosylation profile	615:643	Since modifications of IgG N-glycosylation have been described in some autoimmune diseases, we hypothesized that changes in the pathogenic activity of pemphigus IgG could be related to changes in their N-glycosylation profile.
34680528	6	5	theme	clinical	1073:1080	arg1	activity					1082:1089	pemphigus clinical activity	1063:1089	pemphigus clinical activity	1063:1089	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	6	6	dep	correlation	1043:1053	arg1	i					1038:1038	i	1038:1038	i	1038:1038	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	5	7	theme	IgG	977:979	arg1	N-glycans					981:989	IgG N-glycans	977:989	IgG N-glycans	977:989	The pathogenic activity of serum IgG was measured in vitro on immortalized keratinocytes, by immunofluorescence and dissociation assays, and IgG N-glycans were analyzed by mass spectrometry.
34680528	4	8	theme	N-glycosylation	754:768	arg1	profile					770:776	their N-glycosylation profile	748:776	their N-glycosylation profile	748:776	The purpose of this study was to assess, longitudinally, the pathogenicity of pemphigus serum IgG and their N-glycosylation profile during phases of disease activity and clinical remission.
34680528	6	9	from	patients	1249:1256	arg1	remission					1270:1278	clinical remission	1261:1278	clinical remission	1261:1278	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	6	10	theme	pemphigus	1063:1071	arg1	activity					1082:1089	pemphigus clinical activity	1063:1089	pemphigus clinical activity	1063:1089	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	0	11	theme	Corticosteroid	112:125	arg1	Treatment					127:135	Corticosteroid Treatment	112:135	Corticosteroid Treatment	112:135	Longitudinal Pathogenic Properties and N-Glycosylation Profile of Antibodies from Patients with Pemphigus after Corticosteroid Treatment.
34680528	6	12	theme	relapse	1308:1314	arg1	predictive					1294:1303	predictive	1294:1303	predictive	1294:1303	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	6	12	theme	relapse	1308:1314	arg1	persistence					1164:1174	the persistence	1160:1174	the persistence	1160:1174	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	6	13	from	month	1145:1149	arg1	correlation					1043:1053	a correlation	1041:1053	(i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6	1037:1151	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	6	14	from	disparity	1526:1534	arg1	N-glycans					1543:1551	IgG N-glycans	1539:1551	IgG N-glycans during the course of pemphigus	1539:1582	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	6	15	theme	pathogenic	1451:1460	arg1	related					1511:1517	related	1511:1517	related	1511:1517	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	6	15	theme	pathogenic	1451:1460	arg1	properties					1462:1471	the pathogenic properties	1447:1471	the pathogenic properties of pemphigus IgG	1447:1488	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	4	16	theme	study	666:670	arg1	purpose					650:656	The purpose	646:656	The purpose of this study	646:670	The purpose of this study was to assess, longitudinally, the pathogenicity of pemphigus serum IgG and their N-glycosylation profile during phases of disease activity and clinical remission.
34680528	6	17	dep	altered	1372:1378	arg1	ii					1318:1319	ii	1318:1319	ii	1318:1319	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	6	17	dep	altered	1372:1378	arg1	that					1442:1445	that	1442:1445	that	1442:1445	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	6	17	dep	altered	1372:1378	arg1	iii					1437:1439	iii	1437:1439	iii	1437:1439	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	6	17	dep	altered	1372:1378	arg1	appear					1498:1503	appear	1498:1503	did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus	1490:1582	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	6	18	from	persistence	1164:1174	arg1	patients					1249:1256	patients	1249:1256	patients in clinical remission	1249:1278	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	0	19	with	Patients	82:89	arg1	Pemphigus					96:104	Pemphigus	96:104	Pemphigus after Corticosteroid Treatment	96:135	Longitudinal Pathogenic Properties and N-Glycosylation Profile of Antibodies from Patients with Pemphigus after Corticosteroid Treatment.
34680528	4	20	theme	disease	795:801	arg1	activity					803:810	disease activity	795:810	disease activity	795:810	The purpose of this study was to assess, longitudinally, the pathogenicity of pemphigus serum IgG and their N-glycosylation profile during phases of disease activity and clinical remission.
34680528	6	21	theme	IgG	1539:1541	arg1	N-glycans					1543:1551	IgG N-glycans	1539:1551	IgG N-glycans during the course of pemphigus	1539:1582	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	6	22	theme	in	1183:1184	arg1	activity					1203:1210	the in vitro pathogenic activity	1179:1210	the in vitro pathogenic activity of IgG during its evolution	1179:1238	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	6	23	theme	IgG	1122:1124	arg1	pathogenicity					1099:1111	the pathogenicity	1095:1111	the pathogenicity of serum IgG at baseline	1095:1136	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	4	24	theme	IgG	740:742	arg1	pathogenicity					707:719	the pathogenicity	703:719	the pathogenicity of pemphigus serum IgG and their N-glycosylation profile	703:776	The purpose of this study was to assess, longitudinally, the pathogenicity of pemphigus serum IgG and their N-glycosylation profile during phases of disease activity and clinical remission.
34680528	4	25	theme	pemphigus	724:732	arg1	IgG					740:742	pemphigus serum IgG	724:742	pemphigus serum IgG	724:742	The purpose of this study was to assess, longitudinally, the pathogenicity of pemphigus serum IgG and their N-glycosylation profile during phases of disease activity and clinical remission.
34680528	0	26	from	Profile	55:61	arg1	Patients					82:89	Patients	82:89	Patients with Pemphigus after Corticosteroid Treatment	82:135	Longitudinal Pathogenic Properties and N-Glycosylation Profile of Antibodies from Patients with Pemphigus after Corticosteroid Treatment.
34680528	6	27	theme	IgG	1215:1217	arg1	activity					1203:1210	the in vitro pathogenic activity	1179:1210	the in vitro pathogenic activity of IgG during its evolution	1179:1238	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	0	28	theme	Pathogenic	13:22	arg1	Properties					24:33	Longitudinal Pathogenic Properties	0:33	Longitudinal Pathogenic Properties	0:33	Longitudinal Pathogenic Properties and N-Glycosylation Profile of Antibodies from Patients with Pemphigus after Corticosteroid Treatment.
34680528	6	29	dep	patients	1410:1417	arg1	autoantibodies					1420:1433	autoantibodies	1420:1433	patients' autoantibodies	1410:1433	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	4	30	theme	remission	825:833	arg1	phases					785:790	phases	785:790	phases of disease activity and clinical remission	785:833	The purpose of this study was to assess, longitudinally, the pathogenicity of pemphigus serum IgG and their N-glycosylation profile during phases of disease activity and clinical remission.
34680528	1	31	theme	pathogenic	201:210	arg1	autoantibodies					212:225	pathogenic autoantibodies	201:225	pathogenic autoantibodies that recognize the following epidermal adhesion proteins: desmogleins	201:295	Pemphigus vulgaris is an autoimmune disease that occurs due to pathogenic autoantibodies that recognize the following epidermal adhesion proteins: desmogleins.
34680528	0	32	theme	Longitudinal	0:11	arg1	Properties					24:33	Longitudinal Pathogenic Properties	0:33	Longitudinal Pathogenic Properties	0:33	Longitudinal Pathogenic Properties and N-Glycosylation Profile of Antibodies from Patients with Pemphigus after Corticosteroid Treatment.
34680528	2	33	theme	autoantibodies	370:383	arg1	titers					344:349	the titers	340:349	the titers of anti-desmoglein autoantibodies	340:383	Systemic corticosteroids usually decrease the titers of anti-desmoglein autoantibodies and improve patients' conditions.
34680528	4	34	theme	serum	734:738	arg1	IgG					740:742	pemphigus serum IgG	724:742	pemphigus serum IgG	724:742	The purpose of this study was to assess, longitudinally, the pathogenicity of pemphigus serum IgG and their N-glycosylation profile during phases of disease activity and clinical remission.
34680528	3	35	theme	IgG	442:444	arg1	N-glycosylation					446:460	IgG N-glycosylation	442:460	IgG N-glycosylation	442:460	Since modifications of IgG N-glycosylation have been described in some autoimmune diseases, we hypothesized that changes in the pathogenic activity of pemphigus IgG could be related to changes in their N-glycosylation profile.
34680528	4	36	theme	activity	803:810	arg1	phases					785:790	phases	785:790	phases of disease activity and clinical remission	785:833	The purpose of this study was to assess, longitudinally, the pathogenicity of pemphigus serum IgG and their N-glycosylation profile during phases of disease activity and clinical remission.
34680528	6	37	theme	structure	1357:1365	arg1	modifications					1327:1339	modifications	1327:1339	modifications of the N-glycan structure	1327:1365	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	3	38	theme	N-glycosylation	621:635	arg1	profile					637:643	their N-glycosylation profile	615:643	their N-glycosylation profile	615:643	Since modifications of IgG N-glycosylation have been described in some autoimmune diseases, we hypothesized that changes in the pathogenic activity of pemphigus IgG could be related to changes in their N-glycosylation profile.
34680528	5	39	theme	immortalized	898:909	arg1	keratinocytes					911:923	immortalized keratinocytes	898:923	immortalized keratinocytes	898:923	The pathogenic activity of serum IgG was measured in vitro on immortalized keratinocytes, by immunofluorescence and dissociation assays, and IgG N-glycans were analyzed by mass spectrometry.
34680528	3	40	theme	N-glycosylation	446:460	arg1	modifications					425:437	modifications	425:437	modifications of IgG N-glycosylation	425:460	Since modifications of IgG N-glycosylation have been described in some autoimmune diseases, we hypothesized that changes in the pathogenic activity of pemphigus IgG could be related to changes in their N-glycosylation profile.
34680528	6	41	theme	N-glycan	1348:1355	arg1	structure					1357:1365	the N-glycan structure	1344:1365	the N-glycan structure	1344:1365	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	1	42	dep	proteins	275:282	arg1	desmogleins					285:295	desmogleins	285:295	the following epidermal adhesion proteins: desmogleins	242:295	Pemphigus vulgaris is an autoimmune disease that occurs due to pathogenic autoantibodies that recognize the following epidermal adhesion proteins: desmogleins.
34680528	4	43	theme	clinical	816:823	arg1	remission					825:833	clinical remission	816:833	clinical remission	816:833	The purpose of this study was to assess, longitudinally, the pathogenicity of pemphigus serum IgG and their N-glycosylation profile during phases of disease activity and clinical remission.
34680528	6	44	theme	activity	1203:1210	arg1	predictive					1294:1303	predictive	1294:1303	predictive	1294:1303	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	6	44	theme	activity	1203:1210	arg1	persistence					1164:1174	the persistence	1160:1174	the persistence	1160:1174	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	6	45	theme	serum	1116:1120	arg1	IgG					1122:1124	serum IgG	1116:1124	serum IgG	1116:1124	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	0	46	theme	N-Glycosylation	39:53	arg1	Profile					55:61	N-Glycosylation Profile	39:61	N-Glycosylation Profile	39:61	Longitudinal Pathogenic Properties and N-Glycosylation Profile of Antibodies from Patients with Pemphigus after Corticosteroid Treatment.
34680528	3	47	theme	autoimmune	490:499	arg1	diseases					501:508	some autoimmune diseases	485:508	some autoimmune diseases	485:508	Since modifications of IgG N-glycosylation have been described in some autoimmune diseases, we hypothesized that changes in the pathogenic activity of pemphigus IgG could be related to changes in their N-glycosylation profile.
34680528	0	48	from	Patients	82:89	arg1	Properties					24:33	Longitudinal Pathogenic Properties	0:33	Longitudinal Pathogenic Properties	0:33	Longitudinal Pathogenic Properties and N-Glycosylation Profile of Antibodies from Patients with Pemphigus after Corticosteroid Treatment.
34680528	0	48	from	Patients	82:89	arg1	Profile					55:61	N-Glycosylation Profile	39:61	N-Glycosylation Profile	39:61	Longitudinal Pathogenic Properties and N-Glycosylation Profile of Antibodies from Patients with Pemphigus after Corticosteroid Treatment.
34680528	0	48	from	Patients	82:89	arg1	Antibodies					66:75	Antibodies	66:75	Antibodies from Patients with Pemphigus after Corticosteroid Treatment	66:135	Longitudinal Pathogenic Properties and N-Glycosylation Profile of Antibodies from Patients with Pemphigus after Corticosteroid Treatment.
34680528	1	49	theme	following	246:254	arg1	proteins					275:282	the following epidermal adhesion proteins	242:282	the following epidermal adhesion proteins: desmogleins	242:295	Pemphigus vulgaris is an autoimmune disease that occurs due to pathogenic autoantibodies that recognize the following epidermal adhesion proteins: desmogleins.
34680528	0	50	theme	Antibodies	66:75	arg1	Properties					24:33	Longitudinal Pathogenic Properties	0:33	Longitudinal Pathogenic Properties	0:33	Longitudinal Pathogenic Properties and N-Glycosylation Profile of Antibodies from Patients with Pemphigus after Corticosteroid Treatment.
34680528	0	50	theme	Antibodies	66:75	arg1	Profile					55:61	N-Glycosylation Profile	39:61	N-Glycosylation Profile	39:61	Longitudinal Pathogenic Properties and N-Glycosylation Profile of Antibodies from Patients with Pemphigus after Corticosteroid Treatment.
34680528	5	51	theme	dissociation	952:963	arg1	assays					965:970	dissociation assays	952:970	dissociation assays	952:970	The pathogenic activity of serum IgG was measured in vitro on immortalized keratinocytes, by immunofluorescence and dissociation assays, and IgG N-glycans were analyzed by mass spectrometry.
34680528	6	52	theme	in	1384:1385	arg1	pathogenicity					1393:1405	the in vitro pathogenicity	1380:1405	the in vitro pathogenicity of patients' autoantibodies	1380:1433	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	6	53	theme	clinical	1261:1268	arg1	remission					1270:1278	clinical remission	1261:1278	clinical remission	1261:1278	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	3	54	from	changes	532:538	arg1	activity					558:565	the pathogenic activity	543:565	the pathogenic activity of pemphigus IgG	543:582	Since modifications of IgG N-glycosylation have been described in some autoimmune diseases, we hypothesized that changes in the pathogenic activity of pemphigus IgG could be related to changes in their N-glycosylation profile.
34680528	1	55	theme	Pemphigus	138:146	arg1	disease					174:180	an autoimmune disease	160:180	an autoimmune disease that occurs due to pathogenic autoantibodies that recognize the following epidermal adhesion proteins: desmogleins	160:295	Pemphigus vulgaris is an autoimmune disease that occurs due to pathogenic autoantibodies that recognize the following epidermal adhesion proteins: desmogleins.
34680528	1	55	theme	Pemphigus	138:146	arg1	vulgaris					148:155	Pemphigus vulgaris	138:155	Pemphigus vulgaris	138:155	Pemphigus vulgaris is an autoimmune disease that occurs due to pathogenic autoantibodies that recognize the following epidermal adhesion proteins: desmogleins.
34680528	6	56	theme	pathogenic	1192:1201	arg1	activity					1203:1210	the in vitro pathogenic activity	1179:1210	the in vitro pathogenic activity of IgG during its evolution	1179:1238	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	6	57	theme	pemphigus	1574:1582	arg1	course					1564:1569	the course	1560:1569	the course of pemphigus	1560:1582	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	3	58	theme	pemphigus	570:578	arg1	IgG					580:582	pemphigus IgG	570:582	pemphigus IgG	570:582	Since modifications of IgG N-glycosylation have been described in some autoimmune diseases, we hypothesized that changes in the pathogenic activity of pemphigus IgG could be related to changes in their N-glycosylation profile.
34680528	6	59	dep	in	1384:1385	arg1	vitro					1387:1391	vitro	1387:1391	vitro	1387:1391	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	6	60	from	baseline	1129:1136	arg1	pathogenicity					1099:1111	the pathogenicity	1095:1111	the pathogenicity of serum IgG at baseline	1095:1136	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	3	61	theme	pathogenic	547:556	arg1	activity					558:565	the pathogenic activity	543:565	the pathogenic activity of pemphigus IgG	543:582	Since modifications of IgG N-glycosylation have been described in some autoimmune diseases, we hypothesized that changes in the pathogenic activity of pemphigus IgG could be related to changes in their N-glycosylation profile.
34680528	2	62	theme	anti-desmoglein	354:368	arg1	autoantibodies					370:383	anti-desmoglein autoantibodies	354:383	anti-desmoglein autoantibodies	354:383	Systemic corticosteroids usually decrease the titers of anti-desmoglein autoantibodies and improve patients' conditions.
34680528	1	63	theme	epidermal	256:264	arg1	proteins					275:282	the following epidermal adhesion proteins	242:282	the following epidermal adhesion proteins: desmogleins	242:295	Pemphigus vulgaris is an autoimmune disease that occurs due to pathogenic autoantibodies that recognize the following epidermal adhesion proteins: desmogleins.
34680528	2	64	theme	Systemic	298:305	arg1	corticosteroids					307:321	Systemic corticosteroids	298:321	Systemic corticosteroids	298:321	Systemic corticosteroids usually decrease the titers of anti-desmoglein autoantibodies and improve patients' conditions.
34680528	5	65	theme	mass	1008:1011	arg1	spectrometry					1013:1024	mass spectrometry	1008:1024	mass spectrometry	1008:1024	The pathogenic activity of serum IgG was measured in vitro on immortalized keratinocytes, by immunofluorescence and dissociation assays, and IgG N-glycans were analyzed by mass spectrometry.
34680528	1	66	theme	adhesion	266:273	arg1	proteins					275:282	the following epidermal adhesion proteins	242:282	the following epidermal adhesion proteins: desmogleins	242:295	Pemphigus vulgaris is an autoimmune disease that occurs due to pathogenic autoantibodies that recognize the following epidermal adhesion proteins: desmogleins.
34680528	6	67	dep	in	1183:1184	arg1	vitro					1186:1190	vitro	1186:1190	vitro	1186:1190	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	1	68	theme	autoimmune	163:172	arg1	disease					174:180	an autoimmune disease	160:180	an autoimmune disease that occurs due to pathogenic autoantibodies that recognize the following epidermal adhesion proteins: desmogleins	160:295	Pemphigus vulgaris is an autoimmune disease that occurs due to pathogenic autoantibodies that recognize the following epidermal adhesion proteins: desmogleins.
34680528	1	68	theme	autoimmune	163:172	arg1	vulgaris					148:155	Pemphigus vulgaris	138:155	Pemphigus vulgaris	138:155	Pemphigus vulgaris is an autoimmune disease that occurs due to pathogenic autoantibodies that recognize the following epidermal adhesion proteins: desmogleins.
34680528	6	69	theme	IgG	1486:1488	arg1	related					1511:1517	related	1511:1517	related	1511:1517	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	6	69	theme	IgG	1486:1488	arg1	properties					1462:1471	the pathogenic properties	1447:1471	the pathogenic properties of pemphigus IgG	1447:1488	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
34680528	5	70	theme	IgG	869:871	arg1	activity					851:858	The pathogenic activity	836:858	The pathogenic activity of serum IgG	836:871	The pathogenic activity of serum IgG was measured in vitro on immortalized keratinocytes, by immunofluorescence and dissociation assays, and IgG N-glycans were analyzed by mass spectrometry.
34680528	5	71	theme	serum	863:867	arg1	IgG					869:871	serum IgG	863:871	serum IgG	863:871	The pathogenic activity of serum IgG was measured in vitro on immortalized keratinocytes, by immunofluorescence and dissociation assays, and IgG N-glycans were analyzed by mass spectrometry.
34680528	6	72	theme	pemphigus	1476:1484	arg1	IgG					1486:1488	pemphigus IgG	1476:1488	pemphigus IgG	1476:1488	We showed (i) a correlation between pemphigus clinical activity and the pathogenicity of serum IgG at baseline and at month 6, while the persistence of the in vitro pathogenic activity of IgG during its evolution, even in patients in clinical remission, seemed to be predictive of relapse; (ii) that modifications of the N-glycan structure were altered the in vitro pathogenicity of patients' autoantibodies; (iii) that the pathogenic properties of pemphigus IgG did not appear to be related to the disparity in IgG N-glycans during the course of pemphigus.
32460635	1	0	theme	diseases	383:390	arg1	treatment					356:364	the treatment	352:364	the treatment of neural system diseases	352:390	The replacement therapy or transplantation using neural cells, which differentiated from stem cells, has emerged as a promising strategy for repairing damaged neural tissues and helping functional recovery in the treatment of neural system diseases.
32460635	3	1	theme	regenerative	662:673	arg1	medicine					675:682	regenerative medicine	662:682	regenerative medicine	662:682	This is a key question and has a very significant impact in regenerative medicine.
32460635	4	2	theme	fibroblast	875:884	arg1	FGF2					901:904	FGF2	901:904	FGF2	901:904	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	4	2	theme	fibroblast	875:884	arg1	factor					893:898	basic fibroblast growth factor	869:898	basic fibroblast growth factor (FGF2)	869:905	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	11	3	theme	complex	2333:2339	arg1	nanomimics					2341:2350	HS/FGF2 complex nanomimics	2325:2350	HS/FGF2 complex nanomimics	2325:2350	The AuNP-PMS/FGF2 system presents a new way to achieve HS/FGF2 complex nanomimics efficiently for the neural differentiation of mESCs.
32460635	8	4	theme	pluripotency	1859:1870	arg1	Oct-4					1879:1883	pluripotency marker Oct-4	1859:1883	pluripotency marker Oct-4	1859:1883	Compared with the other materials, the AuNP-PMS/FGF2 composite could significantly stimulate the expression of the specific neural differentiation markers (nestin and β3-tubulin), while obviously down-regulate the mRNA production of pluripotency marker Oct-4 in mESCs.
32460635	1	5	theme	neural	192:197	arg1	cells					199:203	neural cells	192:203	neural cells	192:203	The replacement therapy or transplantation using neural cells, which differentiated from stem cells, has emerged as a promising strategy for repairing damaged neural tissues and helping functional recovery in the treatment of neural system diseases.
32460635	9	6	theme	AuNP-PMS/FGF2	2097:2109	arg1	composite					2111:2119	AuNP-PMS/FGF2 composite	2097:2119	AuNP-PMS/FGF2 composite	2097:2119	Moreover, the promotion effect of the composite on neuronal maturation marker β3-tubulin expression achieved maximally at the low concentration of FGF2 (4 ng/mL), which suggested the high efficiency of AuNP-PMS/FGF2 composite in neural differentiation of mESCs.
32460635	9	7	theme	mESCs	2150:2154	arg1	differentiation					2131:2145	neural differentiation	2124:2145	neural differentiation of mESCs	2124:2154	Moreover, the promotion effect of the composite on neuronal maturation marker β3-tubulin expression achieved maximally at the low concentration of FGF2 (4 ng/mL), which suggested the high efficiency of AuNP-PMS/FGF2 composite in neural differentiation of mESCs.
32460635	8	8	theme	Oct-4	1879:1883	arg1	production					1845:1854	the mRNA production	1836:1854	the mRNA production of pluripotency marker Oct-4 in mESCs	1836:1892	Compared with the other materials, the AuNP-PMS/FGF2 composite could significantly stimulate the expression of the specific neural differentiation markers (nestin and β3-tubulin), while obviously down-regulate the mRNA production of pluripotency marker Oct-4 in mESCs.
32460635	9	9	theme	promotion	1909:1917	arg1	effect					1919:1924	the promotion effect	1905:1924	the promotion effect of the composite on neuronal maturation marker β3-tubulin expression	1905:1993	Moreover, the promotion effect of the composite on neuronal maturation marker β3-tubulin expression achieved maximally at the low concentration of FGF2 (4 ng/mL), which suggested the high efficiency of AuNP-PMS/FGF2 composite in neural differentiation of mESCs.
32460635	0	10	theme	stem	131:134	arg1	cells					136:140	mouse embryonic stem cells	115:140	mouse embryonic stem cells	115:140	Heparin mimics and fibroblast growth factor-2 fabricated nanogold composite in promoting neural differentiation of mouse embryonic stem cells.
32460635	2	11	theme	neuron	531:536	arg1	population					543:552	a neuron cell population	529:552	a neuron cell population	529:552	The challenge, however, is how to control embryonic stem cell fate so that neural differentiation can be efficiently directed to enrich a neuron cell population, and meanwhile to maintain their bioactivities.
32460635	9	12	from	effect	1919:1924	arg1	expression					1984:1993	neuronal maturation marker β3-tubulin expression	1946:1993	neuronal maturation marker β3-tubulin expression	1946:1993	Moreover, the promotion effect of the composite on neuronal maturation marker β3-tubulin expression achieved maximally at the low concentration of FGF2 (4 ng/mL), which suggested the high efficiency of AuNP-PMS/FGF2 composite in neural differentiation of mESCs.
32460635	1	13	theme	stem	232:235	arg1	cells					237:241	stem cells	232:241	stem cells	232:241	The replacement therapy or transplantation using neural cells, which differentiated from stem cells, has emerged as a promising strategy for repairing damaged neural tissues and helping functional recovery in the treatment of neural system diseases.
32460635	9	14	theme	composite	1933:1941	arg1	effect					1919:1924	the promotion effect	1905:1924	the promotion effect of the composite on neuronal maturation marker β3-tubulin expression	1905:1993	Moreover, the promotion effect of the composite on neuronal maturation marker β3-tubulin expression achieved maximally at the low concentration of FGF2 (4 ng/mL), which suggested the high efficiency of AuNP-PMS/FGF2 composite in neural differentiation of mESCs.
32460635	4	15	contain	containing	756:765	arg2	acrylate					848:855	2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate	800:855	2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate	800:855	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	4	15	contain	containing	756:765	arg2	FGF2					901:904	FGF2	901:904	FGF2	901:904	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	4	15	contain	containing	756:765	arg2	AuNPs					787:791	AuNPs	787:791	AuNPs	787:791	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	4	15	contain	containing	756:765	arg2	poly					795:798	poly	795:798	poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS)	795:862	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	4	15	contain	containing	756:765	arg1	nanocomposite					741:753	a new neural-differentiation inductive nanocomposite	702:753	a new neural-differentiation inductive nanocomposite	702:753	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	4	15	contain	containing	756:765	arg2	nanoparticles					772:784	gold nanoparticles	767:784	gold nanoparticles (AuNPs)	767:792	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	4	15	contain	containing	756:765	arg2	factor					893:898	basic fibroblast growth factor	869:898	basic fibroblast growth factor (FGF2)	869:905	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	4	15	contain	containing	756:765	arg2	PMS					859:861	PMS	859:861	PMS	859:861	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	5	16	theme	AuNP-PMS/FGF2	1022:1034	arg1	composite					1036:1044	this AuNP-PMS/FGF2 composite	1017:1044	this AuNP-PMS/FGF2 composite	1017:1044	In this AuNP-PMS/FGF2 composite, PMS, playing as the high-active mimic of heparin/heparan sulfate (HS), is covalently anchored to AuNPs and bound with FGF2 on the surface of nanoparticles, forming a HS/FGF2 complex nanomimics to facilitate its binding to FGF receptor (FGFR) and promote high neural-inductive activity of mESCs.
32460635	8	17	theme	specific	1741:1748	arg1	β3-tubulin					1793:1802	β3-tubulin	1793:1802	β3-tubulin	1793:1802	Compared with the other materials, the AuNP-PMS/FGF2 composite could significantly stimulate the expression of the specific neural differentiation markers (nestin and β3-tubulin), while obviously down-regulate the mRNA production of pluripotency marker Oct-4 in mESCs.
32460635	8	17	theme	specific	1741:1748	arg1	nestin					1782:1787	nestin	1782:1787	nestin	1782:1787	Compared with the other materials, the AuNP-PMS/FGF2 composite could significantly stimulate the expression of the specific neural differentiation markers (nestin and β3-tubulin), while obviously down-regulate the mRNA production of pluripotency marker Oct-4 in mESCs.
32460635	8	17	theme	specific	1741:1748	arg1	markers					1773:1779	the specific neural differentiation markers	1737:1779	the specific neural differentiation markers (nestin and β3-tubulin)	1737:1803	Compared with the other materials, the AuNP-PMS/FGF2 composite could significantly stimulate the expression of the specific neural differentiation markers (nestin and β3-tubulin), while obviously down-regulate the mRNA production of pluripotency marker Oct-4 in mESCs.
32460635	11	18	theme	mESCs	2398:2402	arg1	differentiation					2379:2393	the neural differentiation	2368:2393	the neural differentiation of mESCs	2368:2402	The AuNP-PMS/FGF2 system presents a new way to achieve HS/FGF2 complex nanomimics efficiently for the neural differentiation of mESCs.
32460635	9	19	theme	neuronal	1946:1953	arg1	expression					1984:1993	neuronal maturation marker β3-tubulin expression	1946:1993	neuronal maturation marker β3-tubulin expression	1946:1993	Moreover, the promotion effect of the composite on neuronal maturation marker β3-tubulin expression achieved maximally at the low concentration of FGF2 (4 ng/mL), which suggested the high efficiency of AuNP-PMS/FGF2 composite in neural differentiation of mESCs.
32460635	4	20	theme	new	704:706	arg1	nanocomposite					741:753	a new neural-differentiation inductive nanocomposite	702:753	a new neural-differentiation inductive nanocomposite	702:753	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	3	21	theme	key	612:614	arg1	question					616:623	a key question	610:623	a key question	610:623	This is a key question and has a very significant impact in regenerative medicine.
32460635	3	21	theme	key	612:614	arg1	This					602:605	This	602:605	This	602:605	This is a key question and has a very significant impact in regenerative medicine.
32460635	8	22	theme	differentiation	1757:1771	arg1	β3-tubulin					1793:1802	β3-tubulin	1793:1802	β3-tubulin	1793:1802	Compared with the other materials, the AuNP-PMS/FGF2 composite could significantly stimulate the expression of the specific neural differentiation markers (nestin and β3-tubulin), while obviously down-regulate the mRNA production of pluripotency marker Oct-4 in mESCs.
32460635	8	22	theme	differentiation	1757:1771	arg1	nestin					1782:1787	nestin	1782:1787	nestin	1782:1787	Compared with the other materials, the AuNP-PMS/FGF2 composite could significantly stimulate the expression of the specific neural differentiation markers (nestin and β3-tubulin), while obviously down-regulate the mRNA production of pluripotency marker Oct-4 in mESCs.
32460635	8	22	theme	differentiation	1757:1771	arg1	markers					1773:1779	the specific neural differentiation markers	1737:1779	the specific neural differentiation markers (nestin and β3-tubulin)	1737:1803	Compared with the other materials, the AuNP-PMS/FGF2 composite could significantly stimulate the expression of the specific neural differentiation markers (nestin and β3-tubulin), while obviously down-regulate the mRNA production of pluripotency marker Oct-4 in mESCs.
32460635	9	23	theme	marker	1966:1971	arg1	expression					1984:1993	neuronal maturation marker β3-tubulin expression	1946:1993	neuronal maturation marker β3-tubulin expression	1946:1993	Moreover, the promotion effect of the composite on neuronal maturation marker β3-tubulin expression achieved maximally at the low concentration of FGF2 (4 ng/mL), which suggested the high efficiency of AuNP-PMS/FGF2 composite in neural differentiation of mESCs.
32460635	5	24	theme	sulfate	1104:1110	arg1	high-active					1067:1077	high-active	1067:1077	high-active	1067:1077	In this AuNP-PMS/FGF2 composite, PMS, playing as the high-active mimic of heparin/heparan sulfate (HS), is covalently anchored to AuNPs and bound with FGF2 on the surface of nanoparticles, forming a HS/FGF2 complex nanomimics to facilitate its binding to FGF receptor (FGFR) and promote high neural-inductive activity of mESCs.
32460635	4	25	theme	embryonic	984:992	arg1	mESCs					1006:1010	mESCs	1006:1010	mESCs	1006:1010	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	4	25	theme	embryonic	984:992	arg1	cells					999:1003	mouse embryonic stem cells	978:1003	mouse embryonic stem cells (mESCs)	978:1011	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	10	26	theme	AuNP-PMS/FGF2	2245:2257	arg1	composite					2259:2267	AuNP-PMS/FGF2 composite	2245:2267	AuNP-PMS/FGF2 composite	2245:2267	Meanwhile, both mESCs and L929 cells showed desirable growth during the incubation with AuNP-PMS/FGF2 composite.
32460635	7	27	theme	room	1531:1534	arg1	temperature					1536:1546	room temperature	1531:1546	room temperature for at least 8 days	1531:1566	The results showed that the AuNP-PMS/FGF2 composite could maintain a long-term stability at room temperature for at least 8 days, and greatly promote the neural differentiation of mESCs.
32460635	4	28	theme	efficient	921:929	arg1	differentiation					959:973	the high efficient directional neural-specific differentiation	912:973	the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs)	912:1011	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	10	29	theme	desirable	2201:2209	arg1	growth					2211:2216	desirable growth	2201:2216	desirable growth	2201:2216	Meanwhile, both mESCs and L929 cells showed desirable growth during the incubation with AuNP-PMS/FGF2 composite.
32460635	4	30	theme	gold	767:770	arg1	AuNPs					787:791	AuNPs	787:791	AuNPs	787:791	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	4	30	theme	gold	767:770	arg1	nanoparticles					772:784	gold nanoparticles	767:784	gold nanoparticles (AuNPs)	767:792	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	5	31	theme	complex	1221:1227	arg1	nanomimics					1229:1238	a HS/FGF2 complex nanomimics	1211:1238	a HS/FGF2 complex nanomimics to facilitate its binding to FGF receptor (FGFR) and promote high neural-inductive activity of mESCs	1211:1339	In this AuNP-PMS/FGF2 composite, PMS, playing as the high-active mimic of heparin/heparan sulfate (HS), is covalently anchored to AuNPs and bound with FGF2 on the surface of nanoparticles, forming a HS/FGF2 complex nanomimics to facilitate its binding to FGF receptor (FGFR) and promote high neural-inductive activity of mESCs.
32460635	10	32	theme	L929	2183:2186	arg1	Meanwhile					2157:2165	Meanwhile	2157:2165	Meanwhile	2157:2165	Meanwhile, both mESCs and L929 cells showed desirable growth during the incubation with AuNP-PMS/FGF2 composite.
32460635	10	32	theme	L929	2183:2186	arg1	cells					2188:2192	L929 cells	2183:2192	L929 cells	2183:2192	Meanwhile, both mESCs and L929 cells showed desirable growth during the incubation with AuNP-PMS/FGF2 composite.
32460635	4	33	theme	neural-specific	943:957	arg1	differentiation					959:973	the high efficient directional neural-specific differentiation	912:973	the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs)	912:1011	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	0	34	theme	mouse	115:119	arg1	cells					136:140	mouse embryonic stem cells	115:140	mouse embryonic stem cells	115:140	Heparin mimics and fibroblast growth factor-2 fabricated nanogold composite in promoting neural differentiation of mouse embryonic stem cells.
32460635	1	35	theme	system	376:381	arg1	diseases					383:390	neural system diseases	369:390	neural system diseases	369:390	The replacement therapy or transplantation using neural cells, which differentiated from stem cells, has emerged as a promising strategy for repairing damaged neural tissues and helping functional recovery in the treatment of neural system diseases.
32460635	2	36	theme	stem	445:448	arg1	fate					455:458	embryonic stem cell fate	435:458	embryonic stem cell fate	435:458	The challenge, however, is how to control embryonic stem cell fate so that neural differentiation can be efficiently directed to enrich a neuron cell population, and meanwhile to maintain their bioactivities.
32460635	4	37	theme	inductive	731:739	arg1	nanocomposite					741:753	a new neural-differentiation inductive nanocomposite	702:753	a new neural-differentiation inductive nanocomposite	702:753	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	8	38	from	production	1845:1854	arg1	mESCs					1888:1892	mESCs	1888:1892	mESCs	1888:1892	Compared with the other materials, the AuNP-PMS/FGF2 composite could significantly stimulate the expression of the specific neural differentiation markers (nestin and β3-tubulin), while obviously down-regulate the mRNA production of pluripotency marker Oct-4 in mESCs.
32460635	9	39	theme	FGF2	2042:2045	arg1	concentration					2025:2037	the low concentration	2017:2037	the low concentration of FGF2 (4 ng/mL), which suggested the high efficiency of AuNP-PMS/FGF2 composite in neural differentiation of mESCs	2017:2154	Moreover, the promotion effect of the composite on neuronal maturation marker β3-tubulin expression achieved maximally at the low concentration of FGF2 (4 ng/mL), which suggested the high efficiency of AuNP-PMS/FGF2 composite in neural differentiation of mESCs.
32460635	1	40	theme	damaged	294:300	arg1	tissues					309:315	damaged neural tissues	294:315	damaged neural tissues	294:315	The replacement therapy or transplantation using neural cells, which differentiated from stem cells, has emerged as a promising strategy for repairing damaged neural tissues and helping functional recovery in the treatment of neural system diseases.
32460635	4	41	theme	cells	999:1003	arg1	differentiation					959:973	the high efficient directional neural-specific differentiation	912:973	the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs)	912:1011	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	4	42	theme	2-methacrylamido	800:815	arg1	poly					795:798	poly	795:798	poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS)	795:862	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	4	42	theme	2-methacrylamido	800:815	arg1	acrylate					848:855	2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate	800:855	2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate	800:855	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	0	43	theme	growth	30:35	arg1	factor-2					37:44	fibroblast growth factor-2	19:44	fibroblast growth factor-2	19:44	Heparin mimics and fibroblast growth factor-2 fabricated nanogold composite in promoting neural differentiation of mouse embryonic stem cells.
32460635	5	44	theme	mESCs	1335:1339	arg1	activity					1323:1330	high neural-inductive activity	1301:1330	high neural-inductive activity of mESCs	1301:1339	In this AuNP-PMS/FGF2 composite, PMS, playing as the high-active mimic of heparin/heparan sulfate (HS), is covalently anchored to AuNPs and bound with FGF2 on the surface of nanoparticles, forming a HS/FGF2 complex nanomimics to facilitate its binding to FGF receptor (FGFR) and promote high neural-inductive activity of mESCs.
32460635	11	45	theme	new	2306:2308	arg1	way					2310:2312	a new way	2304:2312	a new way to achieve HS/FGF2 complex nanomimics efficiently for the neural differentiation of mESCs	2304:2402	The AuNP-PMS/FGF2 system presents a new way to achieve HS/FGF2 complex nanomimics efficiently for the neural differentiation of mESCs.
32460635	3	46	theme	significant	640:650	arg1	impact					652:657	a very significant impact	633:657	a very significant impact in regenerative medicine	633:682	This is a key question and has a very significant impact in regenerative medicine.
32460635	5	47	theme	high	1301:1304	arg1	activity					1323:1330	high neural-inductive activity	1301:1330	high neural-inductive activity of mESCs	1301:1339	In this AuNP-PMS/FGF2 composite, PMS, playing as the high-active mimic of heparin/heparan sulfate (HS), is covalently anchored to AuNPs and bound with FGF2 on the surface of nanoparticles, forming a HS/FGF2 complex nanomimics to facilitate its binding to FGF receptor (FGFR) and promote high neural-inductive activity of mESCs.
32460635	5	48	theme	nanoparticles	1188:1200	arg1	surface					1177:1183	the surface	1173:1183	the surface of nanoparticles	1173:1200	In this AuNP-PMS/FGF2 composite, PMS, playing as the high-active mimic of heparin/heparan sulfate (HS), is covalently anchored to AuNPs and bound with FGF2 on the surface of nanoparticles, forming a HS/FGF2 complex nanomimics to facilitate its binding to FGF receptor (FGFR) and promote high neural-inductive activity of mESCs.
32460635	0	49	theme	nanogold	57:64	arg1	composite					66:74	nanogold composite	57:74	nanogold composite	57:74	Heparin mimics and fibroblast growth factor-2 fabricated nanogold composite in promoting neural differentiation of mouse embryonic stem cells.
32460635	0	50	dep	Heparin	0:6	arg1	mimics					8:13	mimics	8:13	mimics	8:13	Heparin mimics and fibroblast growth factor-2 fabricated nanogold composite in promoting neural differentiation of mouse embryonic stem cells.
32460635	3	51	from	impact	652:657	arg1	medicine					675:682	regenerative medicine	662:682	regenerative medicine	662:682	This is a key question and has a very significant impact in regenerative medicine.
32460635	8	52	theme	mRNA	1840:1843	arg1	production					1845:1854	the mRNA production	1836:1854	the mRNA production of pluripotency marker Oct-4 in mESCs	1836:1892	Compared with the other materials, the AuNP-PMS/FGF2 composite could significantly stimulate the expression of the specific neural differentiation markers (nestin and β3-tubulin), while obviously down-regulate the mRNA production of pluripotency marker Oct-4 in mESCs.
32460635	11	53	theme	HS/FGF2	2325:2331	arg1	nanomimics					2341:2350	HS/FGF2 complex nanomimics	2325:2350	HS/FGF2 complex nanomimics	2325:2350	The AuNP-PMS/FGF2 system presents a new way to achieve HS/FGF2 complex nanomimics efficiently for the neural differentiation of mESCs.
32460635	9	54	theme	composite	2111:2119	arg1	efficiency					2083:2092	the high efficiency	2074:2092	the high efficiency of AuNP-PMS/FGF2 composite in neural differentiation of mESCs	2074:2154	Moreover, the promotion effect of the composite on neuronal maturation marker β3-tubulin expression achieved maximally at the low concentration of FGF2 (4 ng/mL), which suggested the high efficiency of AuNP-PMS/FGF2 composite in neural differentiation of mESCs.
32460635	8	55	theme	other	1644:1648	arg1	materials					1650:1658	the other materials	1640:1658	the other materials	1640:1658	Compared with the other materials, the AuNP-PMS/FGF2 composite could significantly stimulate the expression of the specific neural differentiation markers (nestin and β3-tubulin), while obviously down-regulate the mRNA production of pluripotency marker Oct-4 in mESCs.
32460635	1	56	theme	promising	261:269	arg1	strategy					271:278	a promising strategy	259:278	a promising strategy for repairing damaged neural tissues and helping functional recovery in the treatment of neural system diseases	259:390	The replacement therapy or transplantation using neural cells, which differentiated from stem cells, has emerged as a promising strategy for repairing damaged neural tissues and helping functional recovery in the treatment of neural system diseases.
32460635	4	57	theme	basic	869:873	arg1	FGF2					901:904	FGF2	901:904	FGF2	901:904	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	4	57	theme	basic	869:873	arg1	factor					893:898	basic fibroblast growth factor	869:898	basic fibroblast growth factor (FGF2)	869:905	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	9	58	theme	neural	2124:2129	arg1	differentiation					2131:2145	neural differentiation	2124:2145	neural differentiation of mESCs	2124:2154	Moreover, the promotion effect of the composite on neuronal maturation marker β3-tubulin expression achieved maximally at the low concentration of FGF2 (4 ng/mL), which suggested the high efficiency of AuNP-PMS/FGF2 composite in neural differentiation of mESCs.
32460635	8	59	theme	marker	1872:1877	arg1	Oct-4					1879:1883	pluripotency marker Oct-4	1859:1883	pluripotency marker Oct-4	1859:1883	Compared with the other materials, the AuNP-PMS/FGF2 composite could significantly stimulate the expression of the specific neural differentiation markers (nestin and β3-tubulin), while obviously down-regulate the mRNA production of pluripotency marker Oct-4 in mESCs.
32460635	4	60	theme	mouse	978:982	arg1	mESCs					1006:1010	mESCs	1006:1010	mESCs	1006:1010	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	4	60	theme	mouse	978:982	arg1	cells					999:1003	mouse embryonic stem cells	978:1003	mouse embryonic stem cells (mESCs)	978:1011	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	8	61	theme	AuNP-PMS/FGF2	1665:1677	arg1	composite					1679:1687	the AuNP-PMS/FGF2 composite	1661:1687	the AuNP-PMS/FGF2 composite	1661:1687	Compared with the other materials, the AuNP-PMS/FGF2 composite could significantly stimulate the expression of the specific neural differentiation markers (nestin and β3-tubulin), while obviously down-regulate the mRNA production of pluripotency marker Oct-4 in mESCs.
32460635	7	62	theme	neural	1593:1598	arg1	differentiation					1600:1614	the neural differentiation	1589:1614	the neural differentiation of mESCs	1589:1623	The results showed that the AuNP-PMS/FGF2 composite could maintain a long-term stability at room temperature for at least 8 days, and greatly promote the neural differentiation of mESCs.
32460635	0	63	theme	cells	136:140	arg1	differentiation					96:110	neural differentiation	89:110	neural differentiation of mouse embryonic stem cells	89:140	Heparin mimics and fibroblast growth factor-2 fabricated nanogold composite in promoting neural differentiation of mouse embryonic stem cells.
32460635	2	64	dep	is	417:418	arg1	maintain					572:579	maintain	572:579	to maintain their bioactivities	569:599	The challenge, however, is how to control embryonic stem cell fate so that neural differentiation can be efficiently directed to enrich a neuron cell population, and meanwhile to maintain their bioactivities.
32460635	2	64	dep	is	417:418	arg1	control					427:433	control	427:433	to control embryonic stem cell fate so that neural differentiation can be efficiently directed to enrich a neuron cell population	424:552	The challenge, however, is how to control embryonic stem cell fate so that neural differentiation can be efficiently directed to enrich a neuron cell population, and meanwhile to maintain their bioactivities.
32460635	2	65	theme	cell	538:541	arg1	population					543:552	a neuron cell population	529:552	a neuron cell population	529:552	The challenge, however, is how to control embryonic stem cell fate so that neural differentiation can be efficiently directed to enrich a neuron cell population, and meanwhile to maintain their bioactivities.
32460635	11	66	theme	AuNP-PMS/FGF2	2274:2286	arg1	system					2288:2293	The AuNP-PMS/FGF2 system	2270:2293	The AuNP-PMS/FGF2 system	2270:2293	The AuNP-PMS/FGF2 system presents a new way to achieve HS/FGF2 complex nanomimics efficiently for the neural differentiation of mESCs.
32460635	3	67	contain	has	629:631	arg1	question					616:623	a key question	610:623	a key question	610:623	This is a key question and has a very significant impact in regenerative medicine.
32460635	3	67	contain	has	629:631	arg2	impact					652:657	a very significant impact	633:657	a very significant impact in regenerative medicine	633:682	This is a key question and has a very significant impact in regenerative medicine.
32460635	3	67	contain	has	629:631	arg1	This					602:605	This	602:605	This	602:605	This is a key question and has a very significant impact in regenerative medicine.
32460635	4	68	theme	growth	886:891	arg1	FGF2					901:904	FGF2	901:904	FGF2	901:904	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	4	68	theme	growth	886:891	arg1	factor					893:898	basic fibroblast growth factor	869:898	basic fibroblast growth factor (FGF2)	869:905	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	9	69	from	efficiency	2083:2092	arg1	differentiation					2131:2145	neural differentiation	2124:2145	neural differentiation of mESCs	2124:2154	Moreover, the promotion effect of the composite on neuronal maturation marker β3-tubulin expression achieved maximally at the low concentration of FGF2 (4 ng/mL), which suggested the high efficiency of AuNP-PMS/FGF2 composite in neural differentiation of mESCs.
32460635	9	70	theme	maturation	1955:1964	arg1	expression					1984:1993	neuronal maturation marker β3-tubulin expression	1946:1993	neuronal maturation marker β3-tubulin expression	1946:1993	Moreover, the promotion effect of the composite on neuronal maturation marker β3-tubulin expression achieved maximally at the low concentration of FGF2 (4 ng/mL), which suggested the high efficiency of AuNP-PMS/FGF2 composite in neural differentiation of mESCs.
32460635	8	71	theme	neural	1750:1755	arg1	β3-tubulin					1793:1802	β3-tubulin	1793:1802	β3-tubulin	1793:1802	Compared with the other materials, the AuNP-PMS/FGF2 composite could significantly stimulate the expression of the specific neural differentiation markers (nestin and β3-tubulin), while obviously down-regulate the mRNA production of pluripotency marker Oct-4 in mESCs.
32460635	8	71	theme	neural	1750:1755	arg1	nestin					1782:1787	nestin	1782:1787	nestin	1782:1787	Compared with the other materials, the AuNP-PMS/FGF2 composite could significantly stimulate the expression of the specific neural differentiation markers (nestin and β3-tubulin), while obviously down-regulate the mRNA production of pluripotency marker Oct-4 in mESCs.
32460635	8	71	theme	neural	1750:1755	arg1	markers					1773:1779	the specific neural differentiation markers	1737:1779	the specific neural differentiation markers (nestin and β3-tubulin)	1737:1803	Compared with the other materials, the AuNP-PMS/FGF2 composite could significantly stimulate the expression of the specific neural differentiation markers (nestin and β3-tubulin), while obviously down-regulate the mRNA production of pluripotency marker Oct-4 in mESCs.
32460635	1	72	theme	replacement	147:157	arg1	therapy					159:165	The replacement therapy	143:165	The replacement therapy	143:165	The replacement therapy or transplantation using neural cells, which differentiated from stem cells, has emerged as a promising strategy for repairing damaged neural tissues and helping functional recovery in the treatment of neural system diseases.
32460635	9	73	theme	β3-tubulin	1973:1982	arg1	expression					1984:1993	neuronal maturation marker β3-tubulin expression	1946:1993	neuronal maturation marker β3-tubulin expression	1946:1993	Moreover, the promotion effect of the composite on neuronal maturation marker β3-tubulin expression achieved maximally at the low concentration of FGF2 (4 ng/mL), which suggested the high efficiency of AuNP-PMS/FGF2 composite in neural differentiation of mESCs.
32460635	0	74	theme	neural	89:94	arg1	differentiation					96:110	neural differentiation	89:110	neural differentiation of mouse embryonic stem cells	89:140	Heparin mimics and fibroblast growth factor-2 fabricated nanogold composite in promoting neural differentiation of mouse embryonic stem cells.
32460635	8	75	theme	markers	1773:1779	arg1	expression					1723:1732	the expression	1719:1732	the expression of the specific neural differentiation markers (nestin and β3-tubulin)	1719:1803	Compared with the other materials, the AuNP-PMS/FGF2 composite could significantly stimulate the expression of the specific neural differentiation markers (nestin and β3-tubulin), while obviously down-regulate the mRNA production of pluripotency marker Oct-4 in mESCs.
32460635	2	76	theme	neural	468:473	arg1	differentiation					475:489	neural differentiation	468:489	neural differentiation	468:489	The challenge, however, is how to control embryonic stem cell fate so that neural differentiation can be efficiently directed to enrich a neuron cell population, and meanwhile to maintain their bioactivities.
32460635	11	77	theme	neural	2372:2377	arg1	differentiation					2379:2393	the neural differentiation	2368:2393	the neural differentiation of mESCs	2368:2402	The AuNP-PMS/FGF2 system presents a new way to achieve HS/FGF2 complex nanomimics efficiently for the neural differentiation of mESCs.
32460635	5	78	theme	HS/FGF2	1213:1219	arg1	nanomimics					1229:1238	a HS/FGF2 complex nanomimics	1211:1238	a HS/FGF2 complex nanomimics to facilitate its binding to FGF receptor (FGFR) and promote high neural-inductive activity of mESCs	1211:1339	In this AuNP-PMS/FGF2 composite, PMS, playing as the high-active mimic of heparin/heparan sulfate (HS), is covalently anchored to AuNPs and bound with FGF2 on the surface of nanoparticles, forming a HS/FGF2 complex nanomimics to facilitate its binding to FGF receptor (FGFR) and promote high neural-inductive activity of mESCs.
32460635	7	79	theme	long-term	1508:1516	arg1	stability					1518:1526	a long-term stability	1506:1526	a long-term stability	1506:1526	The results showed that the AuNP-PMS/FGF2 composite could maintain a long-term stability at room temperature for at least 8 days, and greatly promote the neural differentiation of mESCs.
32460635	4	80	theme	high	916:919	arg1	differentiation					959:973	the high efficient directional neural-specific differentiation	912:973	the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs)	912:1011	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	0	81	theme	embryonic	121:129	arg1	cells					136:140	mouse embryonic stem cells	115:140	mouse embryonic stem cells	115:140	Heparin mimics and fibroblast growth factor-2 fabricated nanogold composite in promoting neural differentiation of mouse embryonic stem cells.
32460635	1	82	theme	neural	369:374	arg1	diseases					383:390	neural system diseases	369:390	neural system diseases	369:390	The replacement therapy or transplantation using neural cells, which differentiated from stem cells, has emerged as a promising strategy for repairing damaged neural tissues and helping functional recovery in the treatment of neural system diseases.
32460635	4	83	theme	directional	931:941	arg1	differentiation					959:973	the high efficient directional neural-specific differentiation	912:973	the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs)	912:1011	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	9	84	theme	low	2021:2023	arg1	concentration					2025:2037	the low concentration	2017:2037	the low concentration of FGF2 (4 ng/mL), which suggested the high efficiency of AuNP-PMS/FGF2 composite in neural differentiation of mESCs	2017:2154	Moreover, the promotion effect of the composite on neuronal maturation marker β3-tubulin expression achieved maximally at the low concentration of FGF2 (4 ng/mL), which suggested the high efficiency of AuNP-PMS/FGF2 composite in neural differentiation of mESCs.
32460635	6	85	theme	composite	1397:1405	arg1	bioactivity					1357:1367	bioactivity	1357:1367	bioactivity	1357:1367	The stability, bioactivity and biocompatibility of the composite are investigated in this study.
32460635	6	85	theme	composite	1397:1405	arg1	stability					1346:1354	stability	1346:1354	stability	1346:1354	The stability, bioactivity and biocompatibility of the composite are investigated in this study.
32460635	6	85	theme	composite	1397:1405	arg1	biocompatibility					1373:1388	biocompatibility	1373:1388	biocompatibility	1373:1388	The stability, bioactivity and biocompatibility of the composite are investigated in this study.
32460635	5	86	theme	heparin/heparan	1088:1102	arg1	HS					1113:1114	HS	1113:1114	HS	1113:1114	In this AuNP-PMS/FGF2 composite, PMS, playing as the high-active mimic of heparin/heparan sulfate (HS), is covalently anchored to AuNPs and bound with FGF2 on the surface of nanoparticles, forming a HS/FGF2 complex nanomimics to facilitate its binding to FGF receptor (FGFR) and promote high neural-inductive activity of mESCs.
32460635	5	86	theme	heparin/heparan	1088:1102	arg1	sulfate					1104:1110	heparin/heparan sulfate	1088:1110	heparin/heparan sulfate (HS)	1088:1115	In this AuNP-PMS/FGF2 composite, PMS, playing as the high-active mimic of heparin/heparan sulfate (HS), is covalently anchored to AuNPs and bound with FGF2 on the surface of nanoparticles, forming a HS/FGF2 complex nanomimics to facilitate its binding to FGF receptor (FGFR) and promote high neural-inductive activity of mESCs.
32460635	2	87	theme	embryonic	435:443	arg1	fate					455:458	embryonic stem cell fate	435:458	embryonic stem cell fate	435:458	The challenge, however, is how to control embryonic stem cell fate so that neural differentiation can be efficiently directed to enrich a neuron cell population, and meanwhile to maintain their bioactivities.
32460635	9	88	theme	high	2078:2081	arg1	efficiency					2083:2092	the high efficiency	2074:2092	the high efficiency of AuNP-PMS/FGF2 composite in neural differentiation of mESCs	2074:2154	Moreover, the promotion effect of the composite on neuronal maturation marker β3-tubulin expression achieved maximally at the low concentration of FGF2 (4 ng/mL), which suggested the high efficiency of AuNP-PMS/FGF2 composite in neural differentiation of mESCs.
32460635	4	89	theme	neural-differentiation	708:729	arg1	nanocomposite					741:753	a new neural-differentiation inductive nanocomposite	702:753	a new neural-differentiation inductive nanocomposite	702:753	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	0	90	theme	fibroblast	19:28	arg1	factor-2					37:44	fibroblast growth factor-2	19:44	fibroblast growth factor-2	19:44	Heparin mimics and fibroblast growth factor-2 fabricated nanogold composite in promoting neural differentiation of mouse embryonic stem cells.
32460635	5	91	theme	FGF	1269:1271	arg1	FGFR					1283:1286	FGFR	1283:1286	FGFR	1283:1286	In this AuNP-PMS/FGF2 composite, PMS, playing as the high-active mimic of heparin/heparan sulfate (HS), is covalently anchored to AuNPs and bound with FGF2 on the surface of nanoparticles, forming a HS/FGF2 complex nanomimics to facilitate its binding to FGF receptor (FGFR) and promote high neural-inductive activity of mESCs.
32460635	5	91	theme	FGF	1269:1271	arg1	receptor					1273:1280	FGF receptor	1269:1280	FGF receptor (FGFR)	1269:1287	In this AuNP-PMS/FGF2 composite, PMS, playing as the high-active mimic of heparin/heparan sulfate (HS), is covalently anchored to AuNPs and bound with FGF2 on the surface of nanoparticles, forming a HS/FGF2 complex nanomimics to facilitate its binding to FGF receptor (FGFR) and promote high neural-inductive activity of mESCs.
32460635	1	92	theme	neural	302:307	arg1	tissues					309:315	damaged neural tissues	294:315	damaged neural tissues	294:315	The replacement therapy or transplantation using neural cells, which differentiated from stem cells, has emerged as a promising strategy for repairing damaged neural tissues and helping functional recovery in the treatment of neural system diseases.
32460635	7	93	theme	mESCs	1619:1623	arg1	differentiation					1600:1614	the neural differentiation	1589:1614	the neural differentiation of mESCs	1589:1623	The results showed that the AuNP-PMS/FGF2 composite could maintain a long-term stability at room temperature for at least 8 days, and greatly promote the neural differentiation of mESCs.
32460635	4	94	theme	stem	994:997	arg1	mESCs					1006:1010	mESCs	1006:1010	mESCs	1006:1010	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	4	94	theme	stem	994:997	arg1	cells					999:1003	mouse embryonic stem cells	978:1003	mouse embryonic stem cells (mESCs)	978:1011	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	10	95	with	incubation	2229:2238	arg1	composite					2259:2267	AuNP-PMS/FGF2 composite	2245:2267	AuNP-PMS/FGF2 composite	2245:2267	Meanwhile, both mESCs and L929 cells showed desirable growth during the incubation with AuNP-PMS/FGF2 composite.
32460635	1	96	from	recovery	340:347	arg1	treatment					356:364	the treatment	352:364	the treatment of neural system diseases	352:390	The replacement therapy or transplantation using neural cells, which differentiated from stem cells, has emerged as a promising strategy for repairing damaged neural tissues and helping functional recovery in the treatment of neural system diseases.
32460635	2	97	theme	cell	450:453	arg1	fate					455:458	embryonic stem cell fate	435:458	embryonic stem cell fate	435:458	The challenge, however, is how to control embryonic stem cell fate so that neural differentiation can be efficiently directed to enrich a neuron cell population, and meanwhile to maintain their bioactivities.
32460635	5	98	dep	high-active	1067:1077	arg1	mimic					1079:1083	mimic	1079:1083	mimic	1079:1083	In this AuNP-PMS/FGF2 composite, PMS, playing as the high-active mimic of heparin/heparan sulfate (HS), is covalently anchored to AuNPs and bound with FGF2 on the surface of nanoparticles, forming a HS/FGF2 complex nanomimics to facilitate its binding to FGF receptor (FGFR) and promote high neural-inductive activity of mESCs.
32460635	4	99	theme	glucopyranose-co-3-sulfopropyl	817:846	arg1	poly					795:798	poly	795:798	poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS)	795:862	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	4	99	theme	glucopyranose-co-3-sulfopropyl	817:846	arg1	acrylate					848:855	2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate	800:855	2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate	800:855	Here we proposed a new neural-differentiation inductive nanocomposite, containing gold nanoparticles (AuNPs), poly(2-methacrylamido glucopyranose-co-3-sulfopropyl acrylate) (PMS), and basic fibroblast growth factor (FGF2), for the high efficient directional neural-specific differentiation of mouse embryonic stem cells (mESCs).
32460635	1	100	theme	functional	329:338	arg1	recovery					340:347	functional recovery	329:347	functional recovery in the treatment of neural system diseases	329:390	The replacement therapy or transplantation using neural cells, which differentiated from stem cells, has emerged as a promising strategy for repairing damaged neural tissues and helping functional recovery in the treatment of neural system diseases.
32460635	7	101	theme	AuNP-PMS/FGF2	1467:1479	arg1	composite					1481:1489	the AuNP-PMS/FGF2 composite	1463:1489	the AuNP-PMS/FGF2 composite	1463:1489	The results showed that the AuNP-PMS/FGF2 composite could maintain a long-term stability at room temperature for at least 8 days, and greatly promote the neural differentiation of mESCs.
32460635	8	102	dep	markers	1773:1779	arg1	β3-tubulin					1793:1802	β3-tubulin	1793:1802	β3-tubulin	1793:1802	Compared with the other materials, the AuNP-PMS/FGF2 composite could significantly stimulate the expression of the specific neural differentiation markers (nestin and β3-tubulin), while obviously down-regulate the mRNA production of pluripotency marker Oct-4 in mESCs.
32460635	8	102	dep	markers	1773:1779	arg1	nestin					1782:1787	nestin	1782:1787	nestin	1782:1787	Compared with the other materials, the AuNP-PMS/FGF2 composite could significantly stimulate the expression of the specific neural differentiation markers (nestin and β3-tubulin), while obviously down-regulate the mRNA production of pluripotency marker Oct-4 in mESCs.
32460635	8	102	dep	markers	1773:1779	arg1	markers					1773:1779	the specific neural differentiation markers	1737:1779	the specific neural differentiation markers (nestin and β3-tubulin)	1737:1803	Compared with the other materials, the AuNP-PMS/FGF2 composite could significantly stimulate the expression of the specific neural differentiation markers (nestin and β3-tubulin), while obviously down-regulate the mRNA production of pluripotency marker Oct-4 in mESCs.
32460635	5	103	theme	neural-inductive	1306:1321	arg1	activity					1323:1330	high neural-inductive activity	1301:1330	high neural-inductive activity of mESCs	1301:1339	In this AuNP-PMS/FGF2 composite, PMS, playing as the high-active mimic of heparin/heparan sulfate (HS), is covalently anchored to AuNPs and bound with FGF2 on the surface of nanoparticles, forming a HS/FGF2 complex nanomimics to facilitate its binding to FGF receptor (FGFR) and promote high neural-inductive activity of mESCs.
32385822	4	0	theme	matrix	681:686	arg1	capacity					656:663	the sorption capacity	643:663	the sorption capacity of the alginate matrix that composes the hybrid material	643:720	High calcium concentrations strongly reduce the sorption capacity of the alginate matrix that composes the hybrid material and the Cyanex 272 impregnated into silica powder improves the rare earths' sorption performance in this calcium charged media.
32385822	4	1	theme	alginate	672:679	arg1	matrix					681:686	the alginate matrix	668:686	the alginate matrix	668:686	High calcium concentrations strongly reduce the sorption capacity of the alginate matrix that composes the hybrid material and the Cyanex 272 impregnated into silica powder improves the rare earths' sorption performance in this calcium charged media.
32385822	3	2	theme	rare	542:545	arg1	earths					547:552	rare earths	542:552	rare earths	542:552	The competition among rare earths ions (europium, lanthanum, and lutetium) and among rare earths and calcium or sodium ions was investigated.
32385822	5	3	theme	Thomas	924:929	arg1	model					931:935	Thomas model	924:935	Thomas model	924:935	The experimental breakthrough curves obtained were satisfactory fitted by Thomas model.
32385822	4	4	theme	hybrid	706:711	arg1	material					713:720	the hybrid material	702:720	the hybrid material	702:720	High calcium concentrations strongly reduce the sorption capacity of the alginate matrix that composes the hybrid material and the Cyanex 272 impregnated into silica powder improves the rare earths' sorption performance in this calcium charged media.
32385822	1	5	theme	fixed-bed	282:290	arg1	systems					310:316	fixed-bed column continuous systems	282:316	fixed-bed column continuous systems	282:316	Mesoporous silica impregnate with Cyanex 272 (bis/2,4,4-trimethylpentyl/phosphinic acid) extractant was immobilized into an alginate matrix to obtain a composite sorbent easy to use and applicable in fixed-bed column continuous systems.
32385822	1	6	theme	easy	252:255	arg1	sorbent					244:250	a composite sorbent	232:250	a composite sorbent easy to use and applicable in fixed-bed column continuous systems	232:316	Mesoporous silica impregnate with Cyanex 272 (bis/2,4,4-trimethylpentyl/phosphinic acid) extractant was immobilized into an alginate matrix to obtain a composite sorbent easy to use and applicable in fixed-bed column continuous systems.
32385822	4	7	theme	High	599:602	arg1	concentrations					612:625	High calcium concentrations	599:625	High calcium concentrations	599:625	High calcium concentrations strongly reduce the sorption capacity of the alginate matrix that composes the hybrid material and the Cyanex 272 impregnated into silica powder improves the rare earths' sorption performance in this calcium charged media.
32385822	4	8	theme	silica	758:763	arg1	powder					765:770	silica powder	758:770	silica powder	758:770	High calcium concentrations strongly reduce the sorption capacity of the alginate matrix that composes the hybrid material and the Cyanex 272 impregnated into silica powder improves the rare earths' sorption performance in this calcium charged media.
32385822	4	9	theme	earths	790:795	arg1	performance					807:817	the rare earths' sorption performance	781:817	the rare earths' sorption performance in this calcium charged media	781:847	High calcium concentrations strongly reduce the sorption capacity of the alginate matrix that composes the hybrid material and the Cyanex 272 impregnated into silica powder improves the rare earths' sorption performance in this calcium charged media.
32385822	1	10	theme	Mesoporous	82:91	arg1	impregnate					100:109	Mesoporous silica impregnate	82:109	Mesoporous silica impregnate with Cyanex 272 (bis/2,4,4-trimethylpentyl/phosphinic acid) extractant	82:180	Mesoporous silica impregnate with Cyanex 272 (bis/2,4,4-trimethylpentyl/phosphinic acid) extractant was immobilized into an alginate matrix to obtain a composite sorbent easy to use and applicable in fixed-bed column continuous systems.
32385822	2	11	theme	continuous	440:449	arg1	mode					451:454	batch and continuous mode	430:454	batch and continuous mode	430:454	The sorption efficiency of this material was tested for the recovery of Eu(III) ions from aqueous solutions in batch and continuous mode.
32385822	4	12	theme	charged	835:841	arg1	media					843:847	this calcium charged media	822:847	this calcium charged media	822:847	High calcium concentrations strongly reduce the sorption capacity of the alginate matrix that composes the hybrid material and the Cyanex 272 impregnated into silica powder improves the rare earths' sorption performance in this calcium charged media.
32385822	1	13	theme	silica	93:98	arg1	impregnate					100:109	Mesoporous silica impregnate	82:109	Mesoporous silica impregnate with Cyanex 272 (bis/2,4,4-trimethylpentyl/phosphinic acid) extractant	82:180	Mesoporous silica impregnate with Cyanex 272 (bis/2,4,4-trimethylpentyl/phosphinic acid) extractant was immobilized into an alginate matrix to obtain a composite sorbent easy to use and applicable in fixed-bed column continuous systems.
32385822	1	14	theme	alginate	206:213	arg1	matrix					215:220	an alginate matrix	203:220	an alginate matrix to obtain a composite sorbent easy to use and applicable in fixed-bed column continuous systems	203:316	Mesoporous silica impregnate with Cyanex 272 (bis/2,4,4-trimethylpentyl/phosphinic acid) extractant was immobilized into an alginate matrix to obtain a composite sorbent easy to use and applicable in fixed-bed column continuous systems.
32385822	0	15	theme	Lanthanide	0:9	arg1	recovery					11:18	Lanthanide recovery	0:18	Lanthanide recovery by silica-Cyanex 272 material	0:48	Lanthanide recovery by silica-Cyanex 272 material immobilized in alginate matrix.
32385822	1	16	theme	column	292:297	arg1	systems					310:316	fixed-bed column continuous systems	282:316	fixed-bed column continuous systems	282:316	Mesoporous silica impregnate with Cyanex 272 (bis/2,4,4-trimethylpentyl/phosphinic acid) extractant was immobilized into an alginate matrix to obtain a composite sorbent easy to use and applicable in fixed-bed column continuous systems.
32385822	4	17	theme	calcium	827:833	arg1	media					843:847	this calcium charged media	822:847	this calcium charged media	822:847	High calcium concentrations strongly reduce the sorption capacity of the alginate matrix that composes the hybrid material and the Cyanex 272 impregnated into silica powder improves the rare earths' sorption performance in this calcium charged media.
32385822	2	18	theme	Eu	391:392	arg1	ions					399:402	Eu(III) ions	391:402	Eu(III) ions	391:402	The sorption efficiency of this material was tested for the recovery of Eu(III) ions from aqueous solutions in batch and continuous mode.
32385822	0	19	theme	silica-Cyanex	23:35	arg1	material					41:48	silica-Cyanex 272 material	23:48	silica-Cyanex 272 material	23:48	Lanthanide recovery by silica-Cyanex 272 material immobilized in alginate matrix.
32385822	1	20	theme	continuous	299:308	arg1	systems					310:316	fixed-bed column continuous systems	282:316	fixed-bed column continuous systems	282:316	Mesoporous silica impregnate with Cyanex 272 (bis/2,4,4-trimethylpentyl/phosphinic acid) extractant was immobilized into an alginate matrix to obtain a composite sorbent easy to use and applicable in fixed-bed column continuous systems.
32385822	2	21	from	recovery	379:386	arg1	mode					451:454	batch and continuous mode	430:454	batch and continuous mode	430:454	The sorption efficiency of this material was tested for the recovery of Eu(III) ions from aqueous solutions in batch and continuous mode.
32385822	2	22	theme	sorption	323:330	arg1	efficiency					332:341	The sorption efficiency	319:341	The sorption efficiency of this material	319:358	The sorption efficiency of this material was tested for the recovery of Eu(III) ions from aqueous solutions in batch and continuous mode.
32385822	4	23	theme	calcium	604:610	arg1	concentrations					612:625	High calcium concentrations	599:625	High calcium concentrations	599:625	High calcium concentrations strongly reduce the sorption capacity of the alginate matrix that composes the hybrid material and the Cyanex 272 impregnated into silica powder improves the rare earths' sorption performance in this calcium charged media.
32385822	1	24	with	impregnate	100:109	arg1	extractant					171:180	Cyanex 272 (bis/2,4,4-trimethylpentyl/phosphinic acid) extractant	116:180	Cyanex 272 (bis/2,4,4-trimethylpentyl/phosphinic acid) extractant	116:180	Mesoporous silica impregnate with Cyanex 272 (bis/2,4,4-trimethylpentyl/phosphinic acid) extractant was immobilized into an alginate matrix to obtain a composite sorbent easy to use and applicable in fixed-bed column continuous systems.
32385822	1	25	theme	Cyanex	116:121	arg1	extractant					171:180	Cyanex 272 (bis/2,4,4-trimethylpentyl/phosphinic acid) extractant	116:180	Cyanex 272 (bis/2,4,4-trimethylpentyl/phosphinic acid) extractant	116:180	Mesoporous silica impregnate with Cyanex 272 (bis/2,4,4-trimethylpentyl/phosphinic acid) extractant was immobilized into an alginate matrix to obtain a composite sorbent easy to use and applicable in fixed-bed column continuous systems.
32385822	2	26	theme	aqueous	409:415	arg1	solutions					417:425	aqueous solutions	409:425	aqueous solutions	409:425	The sorption efficiency of this material was tested for the recovery of Eu(III) ions from aqueous solutions in batch and continuous mode.
32385822	4	27	theme	sorption	647:654	arg1	capacity					656:663	the sorption capacity	643:663	the sorption capacity of the alginate matrix that composes the hybrid material	643:720	High calcium concentrations strongly reduce the sorption capacity of the alginate matrix that composes the hybrid material and the Cyanex 272 impregnated into silica powder improves the rare earths' sorption performance in this calcium charged media.
32385822	1	28	from	applicable	268:277	arg1	systems					310:316	fixed-bed column continuous systems	282:316	fixed-bed column continuous systems	282:316	Mesoporous silica impregnate with Cyanex 272 (bis/2,4,4-trimethylpentyl/phosphinic acid) extractant was immobilized into an alginate matrix to obtain a composite sorbent easy to use and applicable in fixed-bed column continuous systems.
32385822	2	29	theme	batch	430:434	arg1	mode					451:454	batch and continuous mode	430:454	batch and continuous mode	430:454	The sorption efficiency of this material was tested for the recovery of Eu(III) ions from aqueous solutions in batch and continuous mode.
32385822	4	30	from	performance	807:817	arg1	media					843:847	this calcium charged media	822:847	this calcium charged media	822:847	High calcium concentrations strongly reduce the sorption capacity of the alginate matrix that composes the hybrid material and the Cyanex 272 impregnated into silica powder improves the rare earths' sorption performance in this calcium charged media.
32385822	1	31	theme	composite	234:242	arg1	sorbent					244:250	a composite sorbent	232:250	a composite sorbent easy to use and applicable in fixed-bed column continuous systems	232:316	Mesoporous silica impregnate with Cyanex 272 (bis/2,4,4-trimethylpentyl/phosphinic acid) extractant was immobilized into an alginate matrix to obtain a composite sorbent easy to use and applicable in fixed-bed column continuous systems.
32385822	4	32	theme	sorption	798:805	arg1	performance					807:817	the rare earths' sorption performance	781:817	the rare earths' sorption performance in this calcium charged media	781:847	High calcium concentrations strongly reduce the sorption capacity of the alginate matrix that composes the hybrid material and the Cyanex 272 impregnated into silica powder improves the rare earths' sorption performance in this calcium charged media.
32385822	3	33	theme	earths	484:489	arg1	lutetium					522:529	lutetium	522:529	lutetium	522:529	The competition among rare earths ions (europium, lanthanum, and lutetium) and among rare earths and calcium or sodium ions was investigated.
32385822	3	33	theme	earths	484:489	arg1	europium					497:504	europium	497:504	europium	497:504	The competition among rare earths ions (europium, lanthanum, and lutetium) and among rare earths and calcium or sodium ions was investigated.
32385822	3	33	theme	earths	484:489	arg1	ions					491:494	rare earths ions	479:494	rare earths ions (europium, lanthanum, and lutetium)	479:530	The competition among rare earths ions (europium, lanthanum, and lutetium) and among rare earths and calcium or sodium ions was investigated.
32385822	3	33	theme	earths	484:489	arg1	lanthanum					507:515	lanthanum	507:515	lanthanum	507:515	The competition among rare earths ions (europium, lanthanum, and lutetium) and among rare earths and calcium or sodium ions was investigated.
32385822	1	34	from	use	260:262	arg1	systems					310:316	fixed-bed column continuous systems	282:316	fixed-bed column continuous systems	282:316	Mesoporous silica impregnate with Cyanex 272 (bis/2,4,4-trimethylpentyl/phosphinic acid) extractant was immobilized into an alginate matrix to obtain a composite sorbent easy to use and applicable in fixed-bed column continuous systems.
32385822	3	35	theme	calcium	558:564	arg1	ions					576:579	calcium or sodium ions	558:579	calcium or sodium ions	558:579	The competition among rare earths ions (europium, lanthanum, and lutetium) and among rare earths and calcium or sodium ions was investigated.
32385822	3	36	theme	sodium	569:574	arg1	ions					576:579	calcium or sodium ions	558:579	calcium or sodium ions	558:579	The competition among rare earths ions (europium, lanthanum, and lutetium) and among rare earths and calcium or sodium ions was investigated.
32385822	1	37	theme	bis/2,4,4-trimethylpentyl/phosphinic	128:163	arg1	acid					165:168	bis/2,4,4-trimethylpentyl/phosphinic acid	128:168	bis/2,4,4-trimethylpentyl/phosphinic acid	128:168	Mesoporous silica impregnate with Cyanex 272 (bis/2,4,4-trimethylpentyl/phosphinic acid) extractant was immobilized into an alginate matrix to obtain a composite sorbent easy to use and applicable in fixed-bed column continuous systems.
32385822	1	37	theme	bis/2,4,4-trimethylpentyl/phosphinic	128:163	arg1	Cyanex					116:121	Cyanex	116:121	Cyanex	116:121	Mesoporous silica impregnate with Cyanex 272 (bis/2,4,4-trimethylpentyl/phosphinic acid) extractant was immobilized into an alginate matrix to obtain a composite sorbent easy to use and applicable in fixed-bed column continuous systems.
32385822	5	38	theme	experimental	854:865	arg1	curves					880:885	The experimental breakthrough curves	850:885	The experimental breakthrough curves obtained	850:894	The experimental breakthrough curves obtained were satisfactory fitted by Thomas model.
32385822	3	39	dep	ions	491:494	arg1	lutetium					522:529	lutetium	522:529	lutetium	522:529	The competition among rare earths ions (europium, lanthanum, and lutetium) and among rare earths and calcium or sodium ions was investigated.
32385822	3	39	dep	ions	491:494	arg1	europium					497:504	europium	497:504	europium	497:504	The competition among rare earths ions (europium, lanthanum, and lutetium) and among rare earths and calcium or sodium ions was investigated.
32385822	3	39	dep	ions	491:494	arg1	ions					491:494	rare earths ions	479:494	rare earths ions (europium, lanthanum, and lutetium)	479:530	The competition among rare earths ions (europium, lanthanum, and lutetium) and among rare earths and calcium or sodium ions was investigated.
32385822	3	39	dep	ions	491:494	arg1	lanthanum					507:515	lanthanum	507:515	lanthanum	507:515	The competition among rare earths ions (europium, lanthanum, and lutetium) and among rare earths and calcium or sodium ions was investigated.
32385822	3	40	theme	rare	479:482	arg1	lutetium					522:529	lutetium	522:529	lutetium	522:529	The competition among rare earths ions (europium, lanthanum, and lutetium) and among rare earths and calcium or sodium ions was investigated.
32385822	3	40	theme	rare	479:482	arg1	europium					497:504	europium	497:504	europium	497:504	The competition among rare earths ions (europium, lanthanum, and lutetium) and among rare earths and calcium or sodium ions was investigated.
32385822	3	40	theme	rare	479:482	arg1	ions					491:494	rare earths ions	479:494	rare earths ions (europium, lanthanum, and lutetium)	479:530	The competition among rare earths ions (europium, lanthanum, and lutetium) and among rare earths and calcium or sodium ions was investigated.
32385822	3	40	theme	rare	479:482	arg1	lanthanum					507:515	lanthanum	507:515	lanthanum	507:515	The competition among rare earths ions (europium, lanthanum, and lutetium) and among rare earths and calcium or sodium ions was investigated.
32385822	2	41	theme	material	351:358	arg1	efficiency					332:341	The sorption efficiency	319:341	The sorption efficiency of this material	319:358	The sorption efficiency of this material was tested for the recovery of Eu(III) ions from aqueous solutions in batch and continuous mode.
32385822	2	42	theme	ions	399:402	arg1	recovery					379:386	the recovery	375:386	the recovery of Eu(III) ions from aqueous solutions in batch and continuous mode	375:454	The sorption efficiency of this material was tested for the recovery of Eu(III) ions from aqueous solutions in batch and continuous mode.
32385822	4	43	theme	rare	785:788	arg1	performance					807:817	the rare earths' sorption performance	781:817	the rare earths' sorption performance in this calcium charged media	781:847	High calcium concentrations strongly reduce the sorption capacity of the alginate matrix that composes the hybrid material and the Cyanex 272 impregnated into silica powder improves the rare earths' sorption performance in this calcium charged media.
32385822	5	44	theme	breakthrough	867:878	arg1	curves					880:885	The experimental breakthrough curves	850:885	The experimental breakthrough curves obtained	850:894	The experimental breakthrough curves obtained were satisfactory fitted by Thomas model.
32385822	0	45	theme	alginate	65:72	arg1	matrix					74:79	alginate matrix	65:79	alginate matrix	65:79	Lanthanide recovery by silica-Cyanex 272 material immobilized in alginate matrix.
32385822	2	46	from	solutions	417:425	arg1	recovery					379:386	the recovery	375:386	the recovery of Eu(III) ions from aqueous solutions in batch and continuous mode	375:454	The sorption efficiency of this material was tested for the recovery of Eu(III) ions from aqueous solutions in batch and continuous mode.
34980407	0	0	theme	safe	89:92	arg1	delivery					94:101	the safe delivery	85:101	the safe delivery of β-Carotene	85:115	Physicochemical properties and Strength analysis of vitreous encapsulated solids for the safe delivery of β-Carotene.
34980407	7	1	theme	molecular	1188:1196	arg1	mobility					1198:1205	the molecular mobility	1184:1205	the molecular mobility of wall materials	1184:1223	Strength analysis indicated that LBL emulsions lubricated systems and increased the molecular mobility of wall materials.
34980407	5	2	theme	stabilizer	855:864	arg1	characteristics					780:794	the characteristics	776:794	the characteristics	776:794	The MD exhibited the characteristics of not easy to absorb moisture, suitable carrier, and good stabilizer, which could improve the stability of VES systems at studied environmental stresses.
34980407	5	3	from	stresses	941:948	arg1	stability					891:899	the stability	887:899	the stability of VES systems at studied environmental stresses	887:948	The MD exhibited the characteristics of not easy to absorb moisture, suitable carrier, and good stabilizer, which could improve the stability of VES systems at studied environmental stresses.
34980407	9	4	theme	nutrients	1698:1706	arg1	systems					1717:1723	lipophilic nutrients delivery systems	1687:1723	lipophilic nutrients delivery systems with high total solids	1687:1746	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	1	5	from	compounds	150:158	arg1	water					163:167	water	163:167	water	163:167	β-Carotene (β-Car) is insoluble compounds in water and liable to degradation, which has health benefits for human beings.
34980407	6	6	theme	compositional	959:971	arg1	effects					973:979	compositional effects	959:979	compositional effects from MD and environmental stresses	959:1014	Despite compositional effects from MD and environmental stresses, LBL emulsions changed the water sorption behavior of VES as oil dispersion in storage.
34980407	6	7	theme	environmental	993:1005	arg1	stresses					1007:1014	MD and environmental stresses	986:1014	MD and environmental stresses	986:1014	Despite compositional effects from MD and environmental stresses, LBL emulsions changed the water sorption behavior of VES as oil dispersion in storage.
34980407	9	8	theme	wall	1594:1597	arg1	materials					1599:1607	wall materials	1594:1607	wall materials	1594:1607	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	8	9	theme	storage	1358:1364	arg1	30 days					1347:1353	30 days	1347:1353	30 days of storage	1347:1364	Structural collapse, rapid color changes, and β-Car loss were confirmed in VES systems at 0.56 aw from 25 to 45 °C after 30 days of storage.
34980407	5	10	theme	good	850:853	arg1	stabilizer					855:864	good stabilizer	850:864	good stabilizer	850:864	The MD exhibited the characteristics of not easy to absorb moisture, suitable carrier, and good stabilizer, which could improve the stability of VES systems at studied environmental stresses.
34980407	9	11	theme	systems	1717:1723	arg1	stability					1674:1682	stability	1674:1682	stability	1674:1682	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	9	11	theme	systems	1717:1723	arg1	quality					1662:1668	quality	1662:1668	quality	1662:1668	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	4	12	theme	polyelectrolyte	726:740	arg1	shell					742:746	the polyelectrolyte shell	722:746	the polyelectrolyte shell	722:746	Morphological results indicated that the LBL emulsions were formed a spheric shape, in where the polyelectrolyte shell was 30 nm.
34980407	4	12	theme	polyelectrolyte	726:740	arg1	30 nm					752:756	30 nm	752:756	30 nm	752:756	Morphological results indicated that the LBL emulsions were formed a spheric shape, in where the polyelectrolyte shell was 30 nm.
34980407	3	13	theme	vitreous	536:543	arg1	solids					558:563	vitreous encapsulated solids	536:563	vitreous encapsulated solids (VES) using trehalose and maltodextrins (MD) as wall materials	536:626	In this paper, therefore, the LBL emulsions including β-Car were carefully prepared and encapsulated to obtain vitreous encapsulated solids (VES) using trehalose and maltodextrins (MD) as wall materials.
34980407	3	13	theme	vitreous	536:543	arg1	VES					566:568	VES	566:568	VES	566:568	In this paper, therefore, the LBL emulsions including β-Car were carefully prepared and encapsulated to obtain vitreous encapsulated solids (VES) using trehalose and maltodextrins (MD) as wall materials.
34980407	9	14	theme	β-Car	1405:1409	arg1	loss					1411:1414	β-Car loss	1405:1414	β-Car loss	1405:1414	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	6	15	theme	oil	1077:1079	arg1	dispersion					1081:1090	oil dispersion	1077:1090	oil dispersion in storage	1077:1101	Despite compositional effects from MD and environmental stresses, LBL emulsions changed the water sorption behavior of VES as oil dispersion in storage.
34980407	9	16	theme	loss	1411:1414	arg1	kinetics					1416:1423	S and β-Car loss kinetics	1399:1423	S and β-Car loss kinetics	1399:1423	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	2	17	theme	LBL	265:267	arg1	emulsions					270:278	layer-by-layer (LBL) emulsions	249:278	layer-by-layer (LBL) emulsions	249:278	Although layer-by-layer (LBL) emulsions provide a better protection for β-Car towards environmental stresses, the handling and transportation of LBL emulsions still faces restrictions.
34980407	9	18	theme	S	1399:1399	arg1	kinetics					1416:1423	S and β-Car loss kinetics	1399:1423	S and β-Car loss kinetics	1399:1423	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	2	19	theme	LBL	385:387	arg1	emulsions					389:397	LBL emulsions	385:397	LBL emulsions	385:397	Although layer-by-layer (LBL) emulsions provide a better protection for β-Car towards environmental stresses, the handling and transportation of LBL emulsions still faces restrictions.
34980407	0	20	theme	β-Carotene	106:115	arg1	delivery					94:101	the safe delivery	85:101	the safe delivery of β-Carotene	85:115	Physicochemical properties and Strength analysis of vitreous encapsulated solids for the safe delivery of β-Carotene.
34980407	4	21	theme	Morphological	629:641	arg1	results					643:649	Morphological results	629:649	Morphological results	629:649	Morphological results indicated that the LBL emulsions were formed a spheric shape, in where the polyelectrolyte shell was 30 nm.
34980407	9	22	theme	total	1735:1739	arg1	solids					1741:1746	high total solids	1730:1746	high total solids	1730:1746	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	6	23	from	stresses	1007:1014	arg1	effects					973:979	compositional effects	959:979	compositional effects from MD and environmental stresses	959:1014	Despite compositional effects from MD and environmental stresses, LBL emulsions changed the water sorption behavior of VES as oil dispersion in storage.
34980407	9	24	theme	quicker	1497:1503	arg1	mobility					1515:1522	quicker molecular mobility	1497:1522	quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids	1497:1746	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	5	25	theme	carrier	837:843	arg1	characteristics					780:794	the characteristics	776:794	the characteristics	776:794	The MD exhibited the characteristics of not easy to absorb moisture, suitable carrier, and good stabilizer, which could improve the stability of VES systems at studied environmental stresses.
34980407	0	26	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and Strength analysis of vitreous encapsulated solids for the safe delivery of β-Carotene.
34980407	2	27	theme	better	290:295	arg1	protection					297:306	a better protection	288:306	a better protection for β-Car	288:316	Although layer-by-layer (LBL) emulsions provide a better protection for β-Car towards environmental stresses, the handling and transportation of LBL emulsions still faces restrictions.
34980407	8	28	theme	Structural	1226:1235	arg1	collapse					1237:1244	Structural collapse	1226:1244	Structural collapse	1226:1244	Structural collapse, rapid color changes, and β-Car loss were confirmed in VES systems at 0.56 aw from 25 to 45 °C after 30 days of storage.
34980407	0	29	theme	Strength	31:38	arg1	analysis					40:47	Strength analysis	31:47	Strength analysis of vitreous	31:59	Physicochemical properties and Strength analysis of vitreous encapsulated solids for the safe delivery of β-Carotene.
34980407	6	30	theme	water	1043:1047	arg1	behavior					1058:1065	the water sorption behavior	1039:1065	the water sorption behavior of VES	1039:1072	Despite compositional effects from MD and environmental stresses, LBL emulsions changed the water sorption behavior of VES as oil dispersion in storage.
34980407	8	31	theme	β-Car	1272:1276	arg1	loss					1278:1281	β-Car loss	1272:1281	β-Car loss	1272:1281	Structural collapse, rapid color changes, and β-Car loss were confirmed in VES systems at 0.56 aw from 25 to 45 °C after 30 days of storage.
34980407	8	32	from	45 °C	1335:1339	arg1	aw					1321:1322	0.56 aw	1316:1322	0.56 aw from 25 to 45 °C	1316:1339	Structural collapse, rapid color changes, and β-Car loss were confirmed in VES systems at 0.56 aw from 25 to 45 °C after 30 days of storage.
34980407	3	33	theme	encapsulated	545:556	arg1	solids					558:563	vitreous encapsulated solids	536:563	vitreous encapsulated solids (VES) using trehalose and maltodextrins (MD) as wall materials	536:626	In this paper, therefore, the LBL emulsions including β-Car were carefully prepared and encapsulated to obtain vitreous encapsulated solids (VES) using trehalose and maltodextrins (MD) as wall materials.
34980407	3	33	theme	encapsulated	545:556	arg1	VES					566:568	VES	566:568	VES	566:568	In this paper, therefore, the LBL emulsions including β-Car were carefully prepared and encapsulated to obtain vitreous encapsulated solids (VES) using trehalose and maltodextrins (MD) as wall materials.
34980407	9	34	theme	lipophilic	1687:1696	arg1	systems					1717:1723	lipophilic nutrients delivery systems	1687:1723	lipophilic nutrients delivery systems with high total solids	1687:1746	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	5	35	theme	easy	803:806	arg1	moisture					818:825	not easy to absorb moisture	799:825	not easy to absorb moisture	799:825	The MD exhibited the characteristics of not easy to absorb moisture, suitable carrier, and good stabilizer, which could improve the stability of VES systems at studied environmental stresses.
34980407	7	36	theme	LBL	1137:1139	arg1	emulsions					1141:1149	LBL emulsions	1137:1149	LBL emulsions	1137:1149	Strength analysis indicated that LBL emulsions lubricated systems and increased the molecular mobility of wall materials.
34980407	2	37	dep	handling	354:361	arg1	the					350:352	the	350:352	the	350:352	Although layer-by-layer (LBL) emulsions provide a better protection for β-Car towards environmental stresses, the handling and transportation of LBL emulsions still faces restrictions.
34980407	5	38	theme	systems	908:914	arg1	stability					891:899	the stability	887:899	the stability of VES systems at studied environmental stresses	887:948	The MD exhibited the characteristics of not easy to absorb moisture, suitable carrier, and good stabilizer, which could improve the stability of VES systems at studied environmental stresses.
34980407	6	39	theme	LBL	1017:1019	arg1	emulsions					1021:1029	LBL emulsions	1017:1029	LBL emulsions	1017:1029	Despite compositional effects from MD and environmental stresses, LBL emulsions changed the water sorption behavior of VES as oil dispersion in storage.
34980407	9	40	with	sample	1485:1490	arg1	mobility					1515:1522	quicker molecular mobility	1497:1522	quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids	1497:1746	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	9	41	theme	wall	1527:1530	arg1	materials					1532:1540	wall materials	1527:1540	wall materials	1527:1540	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	8	42	dep	45 °C	1335:1339	arg1	to					1332:1333	to	1332:1333	to	1332:1333	Structural collapse, rapid color changes, and β-Car loss were confirmed in VES systems at 0.56 aw from 25 to 45 °C after 30 days of storage.
34980407	6	43	theme	sorption	1049:1056	arg1	behavior					1058:1065	the water sorption behavior	1039:1065	the water sorption behavior of VES	1039:1072	Despite compositional effects from MD and environmental stresses, LBL emulsions changed the water sorption behavior of VES as oil dispersion in storage.
34980407	9	44	theme	-Car	1629:1632	arg1	degradation					1634:1644	the β -Car degradation	1623:1644	the β -Car degradation	1623:1644	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	9	45	dep	quality	1662:1668	arg1	the					1658:1660	the	1658:1660	the	1658:1660	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	9	46	theme	materials	1532:1540	arg1	mobility					1515:1522	quicker molecular mobility	1497:1522	quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids	1497:1746	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	6	47	from	dispersion	1081:1090	arg1	storage					1095:1101	storage	1095:1101	storage	1095:1101	Despite compositional effects from MD and environmental stresses, LBL emulsions changed the water sorption behavior of VES as oil dispersion in storage.
34980407	2	48	theme	emulsions	389:397	arg1	transportation					367:380	transportation	367:380	transportation	367:380	Although layer-by-layer (LBL) emulsions provide a better protection for β-Car towards environmental stresses, the handling and transportation of LBL emulsions still faces restrictions.
34980407	2	48	theme	emulsions	389:397	arg1	handling					354:361	handling	354:361	handling	354:361	Although layer-by-layer (LBL) emulsions provide a better protection for β-Car towards environmental stresses, the handling and transportation of LBL emulsions still faces restrictions.
34980407	9	49	theme	delivery	1708:1715	arg1	systems					1717:1723	lipophilic nutrients delivery systems	1687:1723	lipophilic nutrients delivery systems with high total solids	1687:1746	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	3	50	theme	wall	613:616	arg1	materials					618:626	wall materials	613:626	wall materials	613:626	In this paper, therefore, the LBL emulsions including β-Car were carefully prepared and encapsulated to obtain vitreous encapsulated solids (VES) using trehalose and maltodextrins (MD) as wall materials.
34980407	3	50	theme	wall	613:616	arg1	trehalose					577:585	trehalose	577:585	trehalose	577:585	In this paper, therefore, the LBL emulsions including β-Car were carefully prepared and encapsulated to obtain vitreous encapsulated solids (VES) using trehalose and maltodextrins (MD) as wall materials.
34980407	3	50	theme	wall	613:616	arg1	maltodextrins					591:603	maltodextrins	591:603	maltodextrins	591:603	In this paper, therefore, the LBL emulsions including β-Car were carefully prepared and encapsulated to obtain vitreous encapsulated solids (VES) using trehalose and maltodextrins (MD) as wall materials.
34980407	5	51	theme	studied	919:925	arg1	stresses					941:948	studied environmental stresses	919:948	studied environmental stresses	919:948	The MD exhibited the characteristics of not easy to absorb moisture, suitable carrier, and good stabilizer, which could improve the stability of VES systems at studied environmental stresses.
34980407	6	52	theme	VES	1070:1072	arg1	behavior					1058:1065	the water sorption behavior	1039:1065	the water sorption behavior of VES	1039:1072	Despite compositional effects from MD and environmental stresses, LBL emulsions changed the water sorption behavior of VES as oil dispersion in storage.
34980407	2	53	theme	environmental	326:338	arg1	stresses					340:347	environmental stresses	326:347	environmental stresses	326:347	Although layer-by-layer (LBL) emulsions provide a better protection for β-Car towards environmental stresses, the handling and transportation of LBL emulsions still faces restrictions.
34980407	0	54	theme	vitreous	52:59	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and Strength analysis of vitreous encapsulated solids for the safe delivery of β-Carotene.
34980407	0	54	theme	vitreous	52:59	arg1	analysis					40:47	Strength analysis	31:47	Strength analysis of vitreous	31:59	Physicochemical properties and Strength analysis of vitreous encapsulated solids for the safe delivery of β-Carotene.
34980407	9	55	from	mobility	1582:1589	arg1	materials					1599:1607	wall materials	1594:1607	wall materials	1594:1607	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	9	56	theme	β	1627:1627	arg1	degradation					1634:1644	the β -Car degradation	1623:1644	the β -Car degradation	1623:1644	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	1	57	contain	has	202:204	arg2	benefits					213:220	health benefits	206:220	health benefits	206:220	β-Carotene (β-Car) is insoluble compounds in water and liable to degradation, which has health benefits for human beings.
34980407	1	57	contain	has	202:204	arg1	degradation					183:193	degradation	183:193	degradation	183:193	β-Carotene (β-Car) is insoluble compounds in water and liable to degradation, which has health benefits for human beings.
34980407	9	58	with	systems	1717:1723	arg1	solids					1741:1746	high total solids	1730:1746	high total solids	1730:1746	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	9	59	theme	high	1730:1733	arg1	solids					1741:1746	high total solids	1730:1746	high total solids	1730:1746	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	9	60	theme	molecular	1505:1513	arg1	mobility					1515:1522	quicker molecular mobility	1497:1522	quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids	1497:1746	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	8	61	from	aw	1321:1322	arg1	systems					1305:1311	VES systems	1301:1311	VES systems at 0.56 aw from 25 to 45 °C	1301:1339	Structural collapse, rapid color changes, and β-Car loss were confirmed in VES systems at 0.56 aw from 25 to 45 °C after 30 days of storage.
34980407	7	62	theme	Strength	1104:1111	arg1	analysis					1113:1120	Strength analysis	1104:1120	Strength analysis	1104:1120	Strength analysis indicated that LBL emulsions lubricated systems and increased the molecular mobility of wall materials.
34980407	2	63	theme	layer-by-layer	249:262	arg1	emulsions					270:278	layer-by-layer (LBL) emulsions	249:278	layer-by-layer (LBL) emulsions	249:278	Although layer-by-layer (LBL) emulsions provide a better protection for β-Car towards environmental stresses, the handling and transportation of LBL emulsions still faces restrictions.
34980407	3	64	theme	LBL	455:457	arg1	β-Car					479:483	β-Car	479:483	β-Car	479:483	In this paper, therefore, the LBL emulsions including β-Car were carefully prepared and encapsulated to obtain vitreous encapsulated solids (VES) using trehalose and maltodextrins (MD) as wall materials.
34980407	3	64	theme	LBL	455:457	arg1	emulsions					459:467	the LBL emulsions	451:467	the LBL emulsions including β-Car	451:483	In this paper, therefore, the LBL emulsions including β-Car were carefully prepared and encapsulated to obtain vitreous encapsulated solids (VES) using trehalose and maltodextrins (MD) as wall materials.
34980407	5	65	theme	VES	904:906	arg1	systems					908:914	VES systems	904:914	VES systems	904:914	The MD exhibited the characteristics of not easy to absorb moisture, suitable carrier, and good stabilizer, which could improve the stability of VES systems at studied environmental stresses.
34980407	1	66	theme	health	206:211	arg1	benefits					213:220	health benefits	206:220	health benefits	206:220	β-Carotene (β-Car) is insoluble compounds in water and liable to degradation, which has health benefits for human beings.
34980407	5	67	theme	environmental	927:939	arg1	stresses					941:948	studied environmental stresses	919:948	studied environmental stresses	919:948	The MD exhibited the characteristics of not easy to absorb moisture, suitable carrier, and good stabilizer, which could improve the stability of VES systems at studied environmental stresses.
34980407	4	68	theme	spheric	698:704	arg1	shape					706:710	a spheric shape	696:710	a spheric shape	696:710	Morphological results indicated that the LBL emulsions were formed a spheric shape, in where the polyelectrolyte shell was 30 nm.
34980407	8	69	theme	VES	1301:1303	arg1	systems					1305:1311	VES systems	1301:1311	VES systems at 0.56 aw from 25 to 45 °C	1301:1339	Structural collapse, rapid color changes, and β-Car loss were confirmed in VES systems at 0.56 aw from 25 to 45 °C after 30 days of storage.
34980407	9	70	dep	mobility	1515:1522	arg1	slow					1613:1616	slow	1613:1616	slow	1613:1616	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	9	70	dep	mobility	1515:1522	arg1	improve					1650:1656	improve	1650:1656	improve the quality and stability of lipophilic nutrients delivery systems with high total solids	1650:1746	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	5	71	theme	moisture	818:825	arg1	characteristics					780:794	the characteristics	776:794	the characteristics	776:794	The MD exhibited the characteristics of not easy to absorb moisture, suitable carrier, and good stabilizer, which could improve the stability of VES systems at studied environmental stresses.
34980407	8	72	theme	color	1253:1257	arg1	changes					1259:1265	rapid color changes	1247:1265	rapid color changes	1247:1265	Structural collapse, rapid color changes, and β-Car loss were confirmed in VES systems at 0.56 aw from 25 to 45 °C after 30 days of storage.
34980407	8	73	theme	rapid	1247:1251	arg1	changes					1259:1265	rapid color changes	1247:1265	rapid color changes	1247:1265	Structural collapse, rapid color changes, and β-Car loss were confirmed in VES systems at 0.56 aw from 25 to 45 °C after 30 days of storage.
34980407	4	74	theme	LBL	670:672	arg1	emulsions					674:682	the LBL emulsions	666:682	the LBL emulsions	666:682	Morphological results indicated that the LBL emulsions were formed a spheric shape, in where the polyelectrolyte shell was 30 nm.
34980407	5	75	theme	suitable	828:835	arg1	carrier					837:843	suitable carrier	828:843	suitable carrier	828:843	The MD exhibited the characteristics of not easy to absorb moisture, suitable carrier, and good stabilizer, which could improve the stability of VES systems at studied environmental stresses.
34980407	6	76	theme	MD	986:987	arg1	stresses					1007:1014	MD and environmental stresses	986:1014	MD and environmental stresses	986:1014	Despite compositional effects from MD and environmental stresses, LBL emulsions changed the water sorption behavior of VES as oil dispersion in storage.
34980407	7	77	theme	wall	1210:1213	arg1	materials					1215:1223	wall materials	1210:1223	wall materials	1210:1223	Strength analysis indicated that LBL emulsions lubricated systems and increased the molecular mobility of wall materials.
34980407	9	78	theme	molecular	1572:1580	arg1	mobility					1582:1589	molecular mobility	1572:1589	molecular mobility in wall materials	1572:1607	Besides, a relationship between S and β-Car loss kinetics was established, where the β-Car degraded more rapidly in a sample with quicker molecular mobility of wall materials Therefore, the controlling of molecular mobility in wall materials can slow down the β -Car degradation and improve the quality and stability of lipophilic nutrients delivery systems with high total solids.
34980407	1	79	theme	insoluble	140:148	arg1	compounds					150:158	insoluble compounds	140:158	insoluble compounds in water	140:167	β-Carotene (β-Car) is insoluble compounds in water and liable to degradation, which has health benefits for human beings.
34980407	7	80	theme	materials	1215:1223	arg1	mobility					1198:1205	the molecular mobility	1184:1205	the molecular mobility of wall materials	1184:1223	Strength analysis indicated that LBL emulsions lubricated systems and increased the molecular mobility of wall materials.
34980407	1	81	theme	human	226:230	arg1	beings					232:237	human beings	226:237	human beings	226:237	β-Carotene (β-Car) is insoluble compounds in water and liable to degradation, which has health benefits for human beings.
33006278	3	0	theme	potential	544:552	arg1	applications					554:565	the potential applications	540:565	the potential applications of heparosan	540:578	The characterization of heparosan isolated from both natural producers and engineered-cells are critical steps towards the potential applications of heparosan.
33006278	4	1	theme	chain	636:640	arg1	size					642:645	intact chain size	629:645	intact chain size	629:645	Heparosan is characterized using 1) analysis of intact chain size and polydispersity, and 2) disaccharide composition.
33006278	0	2	theme	Defined	180:186	arg1	Oligomers					188:196	Defined Oligomers	180:196	Defined Oligomers	180:196	Heparosan Chain Characterization: Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers.
33006278	1	3	theme	chemoenzymatic	278:291	arg1	synthesis					293:301	chemoenzymatic synthesis	278:301	chemoenzymatic synthesis of heparin and heparan sulfates	278:333	Heparosan is a non-sulfated polysaccharide and potential applications include, chemoenzymatic synthesis of heparin and heparan sulfates.
33006278	2	4	theme	engineered	402:411	arg1	cells					413:417	engineered cells	402:417	engineered cells	402:417	Heparosan is produced using microbial cells (natural producers or engineered cells).
33006278	0	5	theme	Heparosan	0:8	arg1	Characterization					16:31	Heparosan Chain Characterization	0:31	Heparosan Chain Characterization: Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers.	0:197	Heparosan Chain Characterization: Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers.
33006278	9	6	from	selectivity	1557:1567	arg1	oligomers					1592:1600	heparosan oligomers	1582:1600	heparosan oligomers	1582:1600	This method successfully demonstrated the substrate selectivity of Hep-BP on heparosan oligomers.
33006278	10	7	theme	chain	1659:1663	arg1	mapping					1665:1671	heparosan chain mapping	1649:1671	heparosan chain mapping	1649:1671	This analytical tool could be applied towards heparosan chain mapping and analysis of unnatural sugar moieties in the heparosan chain.
33006278	6	8	theme	His-tagged	989:998	arg1	Hep-3					1000:1004	purified His-tagged Hep-3	980:1004	purified His-tagged Hep-3	980:1004	The partial digestion of E. coli K5 heparosan with purified His-tagged Hep-3 results in oligomers of defined sizes.
33006278	5	9	dep	lyase	795:799	arg1	eliminase					816:824	eliminase	816:824	eliminase	816:824	The current paper describes a novel method for heparosan chain characterization, using heparin lyase III (Hep-3, an eliminase from Flavobacterium heparinum) and heparanase Bp (Hep-Bp, a hydrolase from Burkholderia pseudomallei).
33006278	5	9	dep	lyase	795:799	arg1	Hep-3					806:810	Hep-3	806:810	Hep-3	806:810	The current paper describes a novel method for heparosan chain characterization, using heparin lyase III (Hep-3, an eliminase from Flavobacterium heparinum) and heparanase Bp (Hep-Bp, a hydrolase from Burkholderia pseudomallei).
33006278	8	10	theme	trisaccharide	1469:1481	arg1	presence					1439:1446	the presence	1435:1446	the presence of a minor resistant trisaccharide (GlcNAc-GlcA-GlcNAc)	1435:1502	Hep-Bp specifically cleaves the linkage between d-glucuronic acid (GlcA) and N-acetyl-d-glucosamine (GlcNAc) but not the linkage between 4-deoxy-α-L-threo-hex-4-enopyranosyluronic acid (deltaUA) and GlcNAc, and results in the presence of a minor resistant trisaccharide (GlcNAc-GlcA-GlcNAc).
33006278	4	11	theme	disaccharide	674:685	arg1	composition					687:697	2) disaccharide composition	671:697	2) disaccharide composition	671:697	Heparosan is characterized using 1) analysis of intact chain size and polydispersity, and 2) disaccharide composition.
33006278	5	12	theme	chain	757:761	arg1	characterization					763:778	heparosan chain characterization	747:778	heparosan chain characterization	747:778	The current paper describes a novel method for heparosan chain characterization, using heparin lyase III (Hep-3, an eliminase from Flavobacterium heparinum) and heparanase Bp (Hep-Bp, a hydrolase from Burkholderia pseudomallei).
33006278	6	13	theme	K5	962:963	arg1	heparosan					965:973	E. coli K5 heparosan	954:973	E. coli K5 heparosan	954:973	The partial digestion of E. coli K5 heparosan with purified His-tagged Hep-3 results in oligomers of defined sizes.
33006278	0	14	theme	Bacterial	135:143	arg1	Bp					166:167	a Bacterial Hydrolase Heparanase Bp	133:167	a Bacterial Hydrolase Heparanase Bp	133:167	Heparosan Chain Characterization: Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers.
33006278	6	15	theme	E.	954:955	arg1	heparosan					965:973	E. coli K5 heparosan	954:973	E. coli K5 heparosan	954:973	The partial digestion of E. coli K5 heparosan with purified His-tagged Hep-3 results in oligomers of defined sizes.
33006278	5	16	theme	novel	730:734	arg1	method					736:741	a novel method	728:741	a novel method for heparosan chain characterization	728:778	The current paper describes a novel method for heparosan chain characterization, using heparin lyase III (Hep-3, an eliminase from Flavobacterium heparinum) and heparanase Bp (Hep-Bp, a hydrolase from Burkholderia pseudomallei).
33006278	10	17	from	mapping	1665:1671	arg1	chain					1731:1735	the heparosan chain	1717:1735	the heparosan chain	1717:1735	This analytical tool could be applied towards heparosan chain mapping and analysis of unnatural sugar moieties in the heparosan chain.
33006278	7	18	from	2	1090:1090	arg1	degree					1060:1065	degree	1060:1065	degree of polymerization from 2 to 8	1060:1095	The oligomers (degree of polymerization from 2 to 8, DP2-DP8) are completely digested with purified GST-tagged Hep-Bp and analyzed using gel permeation chromatography.
33006278	7	18	from	2	1090:1090	arg1	oligomers					1049:1057	The oligomers	1045:1057	The oligomers (degree of polymerization from 2 to 8, DP2-DP8)	1045:1105	The oligomers (degree of polymerization from 2 to 8, DP2-DP8) are completely digested with purified GST-tagged Hep-Bp and analyzed using gel permeation chromatography.
33006278	7	18	from	2	1090:1090	arg1	polymerization					1070:1083	polymerization	1070:1083	polymerization from 2 to 8	1070:1095	The oligomers (degree of polymerization from 2 to 8, DP2-DP8) are completely digested with purified GST-tagged Hep-Bp and analyzed using gel permeation chromatography.
33006278	9	19	theme	Hep-BP	1572:1577	arg1	selectivity					1557:1567	the substrate selectivity	1543:1567	the substrate selectivity of Hep-BP on heparosan oligomers	1543:1600	This method successfully demonstrated the substrate selectivity of Hep-BP on heparosan oligomers.
33006278	6	20	theme	defined	1030:1036	arg1	sizes					1038:1042	defined sizes	1030:1042	defined sizes	1030:1042	The partial digestion of E. coli K5 heparosan with purified His-tagged Hep-3 results in oligomers of defined sizes.
33006278	0	21	theme	Heparanase	155:164	arg1	Bp					166:167	a Bacterial Hydrolase Heparanase Bp	133:167	a Bacterial Hydrolase Heparanase Bp	133:167	Heparosan Chain Characterization: Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers.
33006278	8	22	theme	4-deoxy-α-L-threo-hex-4-enopyranosyluronic	1350:1391	arg1	deltaUA					1399:1405	deltaUA	1399:1405	deltaUA	1399:1405	Hep-Bp specifically cleaves the linkage between d-glucuronic acid (GlcA) and N-acetyl-d-glucosamine (GlcNAc) but not the linkage between 4-deoxy-α-L-threo-hex-4-enopyranosyluronic acid (deltaUA) and GlcNAc, and results in the presence of a minor resistant trisaccharide (GlcNAc-GlcA-GlcNAc).
33006278	8	22	theme	4-deoxy-α-L-threo-hex-4-enopyranosyluronic	1350:1391	arg1	acid					1393:1396	4-deoxy-α-L-threo-hex-4-enopyranosyluronic acid	1350:1396	4-deoxy-α-L-threo-hex-4-enopyranosyluronic acid (deltaUA)	1350:1406	Hep-Bp specifically cleaves the linkage between d-glucuronic acid (GlcA) and N-acetyl-d-glucosamine (GlcNAc) but not the linkage between 4-deoxy-α-L-threo-hex-4-enopyranosyluronic acid (deltaUA) and GlcNAc, and results in the presence of a minor resistant trisaccharide (GlcNAc-GlcA-GlcNAc).
33006278	0	23	theme	Heparosan	76:84	arg1	Depolymerization					45:60	Sequential Depolymerization	34:60	Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III	34:127	Heparosan Chain Characterization: Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers.
33006278	0	23	theme	Heparosan	76:84	arg1	Bp					166:167	a Bacterial Hydrolase Heparanase Bp	133:167	a Bacterial Hydrolase Heparanase Bp	133:167	Heparosan Chain Characterization: Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers.
33006278	5	24	theme	current	704:710	arg1	paper					712:716	The current paper	700:716	The current paper	700:716	The current paper describes a novel method for heparosan chain characterization, using heparin lyase III (Hep-3, an eliminase from Flavobacterium heparinum) and heparanase Bp (Hep-Bp, a hydrolase from Burkholderia pseudomallei).
33006278	0	25	theme	Eliminase	101:109	arg1	Lyase					119:123	a Bacterial Eliminase Heparin Lyase III	89:127	a Bacterial Eliminase Heparin Lyase III	89:127	Heparosan Chain Characterization: Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers.
33006278	3	26	theme	heparosan	445:453	arg1	steps					526:530	critical steps	517:530	critical steps towards the potential applications of heparosan	517:578	The characterization of heparosan isolated from both natural producers and engineered-cells are critical steps towards the potential applications of heparosan.
33006278	3	26	theme	heparosan	445:453	arg1	characterization					425:440	The characterization	421:440	The characterization of heparosan isolated from both natural producers and engineered-cells	421:511	The characterization of heparosan isolated from both natural producers and engineered-cells are critical steps towards the potential applications of heparosan.
33006278	7	27	theme	permeation	1186:1195	arg1	chromatography					1197:1210	gel permeation chromatography	1182:1210	gel permeation chromatography	1182:1210	The oligomers (degree of polymerization from 2 to 8, DP2-DP8) are completely digested with purified GST-tagged Hep-Bp and analyzed using gel permeation chromatography.
33006278	10	28	theme	moieties	1705:1712	arg1	analysis					1677:1684	analysis	1677:1684	analysis of unnatural sugar moieties in the heparosan chain	1677:1735	This analytical tool could be applied towards heparosan chain mapping and analysis of unnatural sugar moieties in the heparosan chain.
33006278	10	28	theme	moieties	1705:1712	arg1	mapping					1665:1671	heparosan chain mapping	1649:1671	heparosan chain mapping	1649:1671	This analytical tool could be applied towards heparosan chain mapping and analysis of unnatural sugar moieties in the heparosan chain.
33006278	1	29	theme	heparin	306:312	arg1	synthesis					293:301	chemoenzymatic synthesis	278:301	chemoenzymatic synthesis of heparin and heparan sulfates	278:333	Heparosan is a non-sulfated polysaccharide and potential applications include, chemoenzymatic synthesis of heparin and heparan sulfates.
33006278	3	30	theme	natural	474:480	arg1	producers					482:490	natural producers	474:490	natural producers	474:490	The characterization of heparosan isolated from both natural producers and engineered-cells are critical steps towards the potential applications of heparosan.
33006278	0	31	theme	Sequential	34:43	arg1	Depolymerization					45:60	Sequential Depolymerization	34:60	Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III	34:127	Heparosan Chain Characterization: Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers.
33006278	1	32	theme	heparan	318:324	arg1	sulfates					326:333	heparan sulfates	318:333	heparan sulfates	318:333	Heparosan is a non-sulfated polysaccharide and potential applications include, chemoenzymatic synthesis of heparin and heparan sulfates.
33006278	10	33	from	moieties	1705:1712	arg1	chain					1731:1735	the heparosan chain	1717:1735	the heparosan chain	1717:1735	This analytical tool could be applied towards heparosan chain mapping and analysis of unnatural sugar moieties in the heparosan chain.
33006278	0	34	theme	Coli	68:71	arg1	Heparosan					76:84	E. Coli K5 Heparosan	65:84	E. Coli K5 Heparosan	65:84	Heparosan Chain Characterization: Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers.
33006278	2	35	theme	microbial	364:372	arg1	cells					374:378	microbial cells	364:378	microbial cells (natural producers or engineered cells)	364:418	Heparosan is produced using microbial cells (natural producers or engineered cells).
33006278	7	36	theme	GST-tagged	1145:1154	arg1	Hep-Bp					1156:1161	purified GST-tagged Hep-Bp	1136:1161	purified GST-tagged Hep-Bp	1136:1161	The oligomers (degree of polymerization from 2 to 8, DP2-DP8) are completely digested with purified GST-tagged Hep-Bp and analyzed using gel permeation chromatography.
33006278	1	37	theme	non-sulfated	214:225	arg1	Heparosan					199:207	Heparosan	199:207	Heparosan	199:207	Heparosan is a non-sulfated polysaccharide and potential applications include, chemoenzymatic synthesis of heparin and heparan sulfates.
33006278	1	37	theme	non-sulfated	214:225	arg1	polysaccharide					227:240	a non-sulfated polysaccharide	212:240	a non-sulfated polysaccharide	212:240	Heparosan is a non-sulfated polysaccharide and potential applications include, chemoenzymatic synthesis of heparin and heparan sulfates.
33006278	10	38	from	chain	1731:1735	arg1	analysis					1677:1684	analysis	1677:1684	analysis of unnatural sugar moieties in the heparosan chain	1677:1735	This analytical tool could be applied towards heparosan chain mapping and analysis of unnatural sugar moieties in the heparosan chain.
33006278	10	38	from	chain	1731:1735	arg1	mapping					1665:1671	heparosan chain mapping	1649:1671	heparosan chain mapping	1649:1671	This analytical tool could be applied towards heparosan chain mapping and analysis of unnatural sugar moieties in the heparosan chain.
33006278	6	39	with	digestion	941:949	arg1	Hep-3					1000:1004	purified His-tagged Hep-3	980:1004	purified His-tagged Hep-3	980:1004	The partial digestion of E. coli K5 heparosan with purified His-tagged Hep-3 results in oligomers of defined sizes.
33006278	4	40	theme	size	642:645	arg1	analysis					617:624	analysis	617:624	1) analysis of intact chain size and polydispersity	614:664	Heparosan is characterized using 1) analysis of intact chain size and polydispersity, and 2) disaccharide composition.
33006278	4	40	theme	size	642:645	arg1	composition					687:697	2) disaccharide composition	671:697	2) disaccharide composition	671:697	Heparosan is characterized using 1) analysis of intact chain size and polydispersity, and 2) disaccharide composition.
33006278	10	41	theme	analytical	1608:1617	arg1	tool					1619:1622	This analytical tool	1603:1622	This analytical tool	1603:1622	This analytical tool could be applied towards heparosan chain mapping and analysis of unnatural sugar moieties in the heparosan chain.
33006278	10	42	theme	heparosan	1721:1729	arg1	chain					1731:1735	the heparosan chain	1717:1735	the heparosan chain	1717:1735	This analytical tool could be applied towards heparosan chain mapping and analysis of unnatural sugar moieties in the heparosan chain.
33006278	8	43	theme	resistant	1459:1467	arg1	GlcNAc-GlcA-GlcNAc					1484:1501	GlcNAc-GlcA-GlcNAc	1484:1501	GlcNAc-GlcA-GlcNAc	1484:1501	Hep-Bp specifically cleaves the linkage between d-glucuronic acid (GlcA) and N-acetyl-d-glucosamine (GlcNAc) but not the linkage between 4-deoxy-α-L-threo-hex-4-enopyranosyluronic acid (deltaUA) and GlcNAc, and results in the presence of a minor resistant trisaccharide (GlcNAc-GlcA-GlcNAc).
33006278	8	43	theme	resistant	1459:1467	arg1	trisaccharide					1469:1481	a minor resistant trisaccharide	1451:1481	a minor resistant trisaccharide (GlcNAc-GlcA-GlcNAc)	1451:1502	Hep-Bp specifically cleaves the linkage between d-glucuronic acid (GlcA) and N-acetyl-d-glucosamine (GlcNAc) but not the linkage between 4-deoxy-α-L-threo-hex-4-enopyranosyluronic acid (deltaUA) and GlcNAc, and results in the presence of a minor resistant trisaccharide (GlcNAc-GlcA-GlcNAc).
33006278	0	44	theme	E.	65:66	arg1	Heparosan					76:84	E. Coli K5 Heparosan	65:84	E. Coli K5 Heparosan	65:84	Heparosan Chain Characterization: Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers.
33006278	4	45	dep	composition	687:697	arg1	2					671:671	2	671:671	2	671:671	Heparosan is characterized using 1) analysis of intact chain size and polydispersity, and 2) disaccharide composition.
33006278	4	46	theme	polydispersity	651:664	arg1	analysis					617:624	analysis	617:624	1) analysis of intact chain size and polydispersity	614:664	Heparosan is characterized using 1) analysis of intact chain size and polydispersity, and 2) disaccharide composition.
33006278	4	46	theme	polydispersity	651:664	arg1	composition					687:697	2) disaccharide composition	671:697	2) disaccharide composition	671:697	Heparosan is characterized using 1) analysis of intact chain size and polydispersity, and 2) disaccharide composition.
33006278	7	47	dep	2	1090:1090	arg1	8					1095:1095	8	1095:1095	8	1095:1095	The oligomers (degree of polymerization from 2 to 8, DP2-DP8) are completely digested with purified GST-tagged Hep-Bp and analyzed using gel permeation chromatography.
33006278	7	47	dep	2	1090:1090	arg1	to					1092:1093	to	1092:1093	to	1092:1093	The oligomers (degree of polymerization from 2 to 8, DP2-DP8) are completely digested with purified GST-tagged Hep-Bp and analyzed using gel permeation chromatography.
33006278	6	48	theme	partial	933:939	arg1	digestion					941:949	The partial digestion	929:949	The partial digestion of E. coli K5 heparosan with purified His-tagged Hep-3	929:1004	The partial digestion of E. coli K5 heparosan with purified His-tagged Hep-3 results in oligomers of defined sizes.
33006278	10	49	from	analysis	1677:1684	arg1	chain					1731:1735	the heparosan chain	1717:1735	the heparosan chain	1717:1735	This analytical tool could be applied towards heparosan chain mapping and analysis of unnatural sugar moieties in the heparosan chain.
33006278	4	50	theme	intact	629:634	arg1	size					642:645	intact chain size	629:645	intact chain size	629:645	Heparosan is characterized using 1) analysis of intact chain size and polydispersity, and 2) disaccharide composition.
33006278	7	51	dep	degree	1060:1065	arg1	DP2-DP8					1098:1104	DP2-DP8	1098:1104	DP2-DP8	1098:1104	The oligomers (degree of polymerization from 2 to 8, DP2-DP8) are completely digested with purified GST-tagged Hep-Bp and analyzed using gel permeation chromatography.
33006278	3	52	theme	heparosan	570:578	arg1	applications					554:565	the potential applications	540:565	the potential applications of heparosan	540:578	The characterization of heparosan isolated from both natural producers and engineered-cells are critical steps towards the potential applications of heparosan.
33006278	8	53	theme	d-glucuronic	1261:1272	arg1	GlcA					1280:1283	GlcA	1280:1283	GlcA	1280:1283	Hep-Bp specifically cleaves the linkage between d-glucuronic acid (GlcA) and N-acetyl-d-glucosamine (GlcNAc) but not the linkage between 4-deoxy-α-L-threo-hex-4-enopyranosyluronic acid (deltaUA) and GlcNAc, and results in the presence of a minor resistant trisaccharide (GlcNAc-GlcA-GlcNAc).
33006278	8	53	theme	d-glucuronic	1261:1272	arg1	acid					1274:1277	d-glucuronic acid	1261:1277	d-glucuronic acid (GlcA)	1261:1284	Hep-Bp specifically cleaves the linkage between d-glucuronic acid (GlcA) and N-acetyl-d-glucosamine (GlcNAc) but not the linkage between 4-deoxy-α-L-threo-hex-4-enopyranosyluronic acid (deltaUA) and GlcNAc, and results in the presence of a minor resistant trisaccharide (GlcNAc-GlcA-GlcNAc).
33006278	3	54	attach	isolated	455:462	arg2	heparosan					445:453	heparosan	445:453	heparosan isolated from both natural producers and engineered-cells	445:511	The characterization of heparosan isolated from both natural producers and engineered-cells are critical steps towards the potential applications of heparosan.
33006278	3	54	attach	isolated	455:462	arg1	engineered-cells					496:511	engineered-cells	496:511	engineered-cells	496:511	The characterization of heparosan isolated from both natural producers and engineered-cells are critical steps towards the potential applications of heparosan.
33006278	3	54	attach	isolated	455:462	arg1	producers					482:490	natural producers	474:490	natural producers	474:490	The characterization of heparosan isolated from both natural producers and engineered-cells are critical steps towards the potential applications of heparosan.
33006278	10	55	theme	heparosan	1649:1657	arg1	mapping					1665:1671	heparosan chain mapping	1649:1671	heparosan chain mapping	1649:1671	This analytical tool could be applied towards heparosan chain mapping and analysis of unnatural sugar moieties in the heparosan chain.
33006278	5	56	theme	heparanase	861:870	arg1	Bp					872:873	heparanase Bp	861:873	heparanase Bp (Hep-Bp, a hydrolase from Burkholderia pseudomallei)	861:926	The current paper describes a novel method for heparosan chain characterization, using heparin lyase III (Hep-3, an eliminase from Flavobacterium heparinum) and heparanase Bp (Hep-Bp, a hydrolase from Burkholderia pseudomallei).
33006278	6	57	theme	purified	980:987	arg1	Hep-3					1000:1004	purified His-tagged Hep-3	980:1004	purified His-tagged Hep-3	980:1004	The partial digestion of E. coli K5 heparosan with purified His-tagged Hep-3 results in oligomers of defined sizes.
33006278	9	58	theme	heparosan	1582:1590	arg1	oligomers					1592:1600	heparosan oligomers	1582:1600	heparosan oligomers	1582:1600	This method successfully demonstrated the substrate selectivity of Hep-BP on heparosan oligomers.
33006278	10	59	theme	unnatural	1689:1697	arg1	moieties					1705:1712	unnatural sugar moieties	1689:1712	unnatural sugar moieties in the heparosan chain	1689:1735	This analytical tool could be applied towards heparosan chain mapping and analysis of unnatural sugar moieties in the heparosan chain.
33006278	7	60	theme	polymerization	1070:1083	arg1	degree					1060:1065	degree	1060:1065	degree of polymerization from 2 to 8	1060:1095	The oligomers (degree of polymerization from 2 to 8, DP2-DP8) are completely digested with purified GST-tagged Hep-Bp and analyzed using gel permeation chromatography.
33006278	7	60	theme	polymerization	1070:1083	arg1	oligomers					1049:1057	The oligomers	1045:1057	The oligomers (degree of polymerization from 2 to 8, DP2-DP8)	1045:1105	The oligomers (degree of polymerization from 2 to 8, DP2-DP8) are completely digested with purified GST-tagged Hep-Bp and analyzed using gel permeation chromatography.
33006278	6	61	theme	heparosan	965:973	arg1	digestion					941:949	The partial digestion	929:949	The partial digestion of E. coli K5 heparosan with purified His-tagged Hep-3	929:1004	The partial digestion of E. coli K5 heparosan with purified His-tagged Hep-3 results in oligomers of defined sizes.
33006278	0	62	theme	Hydrolase	145:153	arg1	Bp					166:167	a Bacterial Hydrolase Heparanase Bp	133:167	a Bacterial Hydrolase Heparanase Bp	133:167	Heparosan Chain Characterization: Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers.
33006278	4	63	dep	analysis	617:624	arg1	1					614:614	1	614:614	1	614:614	Heparosan is characterized using 1) analysis of intact chain size and polydispersity, and 2) disaccharide composition.
33006278	6	64	theme	coli	957:960	arg1	heparosan					965:973	E. coli K5 heparosan	954:973	E. coli K5 heparosan	954:973	The partial digestion of E. coli K5 heparosan with purified His-tagged Hep-3 results in oligomers of defined sizes.
33006278	9	65	theme	substrate	1547:1555	arg1	selectivity					1557:1567	the substrate selectivity	1543:1567	the substrate selectivity of Hep-BP on heparosan oligomers	1543:1600	This method successfully demonstrated the substrate selectivity of Hep-BP on heparosan oligomers.
33006278	0	66	theme	K5	73:74	arg1	Heparosan					76:84	E. Coli K5 Heparosan	65:84	E. Coli K5 Heparosan	65:84	Heparosan Chain Characterization: Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers.
33006278	5	67	theme	Burkholderia	901:912	arg1	pseudomallei					914:925	Burkholderia pseudomallei	901:925	Burkholderia pseudomallei	901:925	The current paper describes a novel method for heparosan chain characterization, using heparin lyase III (Hep-3, an eliminase from Flavobacterium heparinum) and heparanase Bp (Hep-Bp, a hydrolase from Burkholderia pseudomallei).
33006278	6	68	theme	sizes	1038:1042	arg1	oligomers					1017:1025	oligomers	1017:1025	oligomers of defined sizes	1017:1042	The partial digestion of E. coli K5 heparosan with purified His-tagged Hep-3 results in oligomers of defined sizes.
33006278	0	69	theme	Bacterial	91:99	arg1	Lyase					119:123	a Bacterial Eliminase Heparin Lyase III	89:127	a Bacterial Eliminase Heparin Lyase III	89:127	Heparosan Chain Characterization: Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers.
33006278	2	70	dep	cells	374:378	arg1	cells					413:417	engineered cells	402:417	engineered cells	402:417	Heparosan is produced using microbial cells (natural producers or engineered cells).
33006278	2	70	dep	cells	374:378	arg1	producers					389:397	natural producers	381:397	natural producers	381:397	Heparosan is produced using microbial cells (natural producers or engineered cells).
33006278	0	71	theme	Heparin	111:117	arg1	Lyase					119:123	a Bacterial Eliminase Heparin Lyase III	89:127	a Bacterial Eliminase Heparin Lyase III	89:127	Heparosan Chain Characterization: Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers.
33006278	5	72	from	pseudomallei	914:925	arg1	Hep-Bp					876:881	Hep-Bp	876:881	Hep-Bp	876:881	The current paper describes a novel method for heparosan chain characterization, using heparin lyase III (Hep-3, an eliminase from Flavobacterium heparinum) and heparanase Bp (Hep-Bp, a hydrolase from Burkholderia pseudomallei).
33006278	5	72	from	pseudomallei	914:925	arg1	hydrolase					886:894	a hydrolase	884:894	a hydrolase from Burkholderia pseudomallei	884:925	The current paper describes a novel method for heparosan chain characterization, using heparin lyase III (Hep-3, an eliminase from Flavobacterium heparinum) and heparanase Bp (Hep-Bp, a hydrolase from Burkholderia pseudomallei).
33006278	0	73	theme	Chain	10:14	arg1	Characterization					16:31	Heparosan Chain Characterization	0:31	Heparosan Chain Characterization: Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers.	0:197	Heparosan Chain Characterization: Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers.
33006278	5	74	from	heparinum	846:854	arg1	eliminase					816:824	eliminase	816:824	eliminase	816:824	The current paper describes a novel method for heparosan chain characterization, using heparin lyase III (Hep-3, an eliminase from Flavobacterium heparinum) and heparanase Bp (Hep-Bp, a hydrolase from Burkholderia pseudomallei).
33006278	5	74	from	heparinum	846:854	arg1	Hep-3					806:810	Hep-3	806:810	Hep-3	806:810	The current paper describes a novel method for heparosan chain characterization, using heparin lyase III (Hep-3, an eliminase from Flavobacterium heparinum) and heparanase Bp (Hep-Bp, a hydrolase from Burkholderia pseudomallei).
33006278	5	75	theme	heparosan	747:755	arg1	characterization					763:778	heparosan chain characterization	747:778	heparosan chain characterization	747:778	The current paper describes a novel method for heparosan chain characterization, using heparin lyase III (Hep-3, an eliminase from Flavobacterium heparinum) and heparanase Bp (Hep-Bp, a hydrolase from Burkholderia pseudomallei).
33006278	10	76	theme	sugar	1699:1703	arg1	moieties					1705:1712	unnatural sugar moieties	1689:1712	unnatural sugar moieties in the heparosan chain	1689:1735	This analytical tool could be applied towards heparosan chain mapping and analysis of unnatural sugar moieties in the heparosan chain.
33006278	5	77	theme	heparin	787:793	arg1	lyase					795:799	heparin lyase III	787:803	heparin lyase III (Hep-3, an eliminase from Flavobacterium heparinum)	787:855	The current paper describes a novel method for heparosan chain characterization, using heparin lyase III (Hep-3, an eliminase from Flavobacterium heparinum) and heparanase Bp (Hep-Bp, a hydrolase from Burkholderia pseudomallei).
33006278	7	78	theme	purified	1136:1143	arg1	Hep-Bp					1156:1161	purified GST-tagged Hep-Bp	1136:1161	purified GST-tagged Hep-Bp	1136:1161	The oligomers (degree of polymerization from 2 to 8, DP2-DP8) are completely digested with purified GST-tagged Hep-Bp and analyzed using gel permeation chromatography.
33006278	1	79	theme	sulfates	326:333	arg1	synthesis					293:301	chemoenzymatic synthesis	278:301	chemoenzymatic synthesis of heparin and heparan sulfates	278:333	Heparosan is a non-sulfated polysaccharide and potential applications include, chemoenzymatic synthesis of heparin and heparan sulfates.
33006278	2	80	theme	natural	381:387	arg1	producers					389:397	natural producers	381:397	natural producers	381:397	Heparosan is produced using microbial cells (natural producers or engineered cells).
33006278	5	81	dep	Bp	872:873	arg1	Hep-Bp					876:881	Hep-Bp	876:881	Hep-Bp	876:881	The current paper describes a novel method for heparosan chain characterization, using heparin lyase III (Hep-3, an eliminase from Flavobacterium heparinum) and heparanase Bp (Hep-Bp, a hydrolase from Burkholderia pseudomallei).
33006278	5	81	dep	Bp	872:873	arg1	hydrolase					886:894	a hydrolase	884:894	a hydrolase from Burkholderia pseudomallei	884:925	The current paper describes a novel method for heparosan chain characterization, using heparin lyase III (Hep-3, an eliminase from Flavobacterium heparinum) and heparanase Bp (Hep-Bp, a hydrolase from Burkholderia pseudomallei).
33006278	3	82	theme	critical	517:524	arg1	steps					526:530	critical steps	517:530	critical steps towards the potential applications of heparosan	517:578	The characterization of heparosan isolated from both natural producers and engineered-cells are critical steps towards the potential applications of heparosan.
33006278	3	82	theme	critical	517:524	arg1	characterization					425:440	The characterization	421:440	The characterization of heparosan isolated from both natural producers and engineered-cells	421:511	The characterization of heparosan isolated from both natural producers and engineered-cells are critical steps towards the potential applications of heparosan.
33006278	7	83	from	degree	1060:1065	arg1	2					1090:1090	2	1090:1090	2	1090:1090	The oligomers (degree of polymerization from 2 to 8, DP2-DP8) are completely digested with purified GST-tagged Hep-Bp and analyzed using gel permeation chromatography.
33006278	8	84	theme	minor	1453:1457	arg1	GlcNAc-GlcA-GlcNAc					1484:1501	GlcNAc-GlcA-GlcNAc	1484:1501	GlcNAc-GlcA-GlcNAc	1484:1501	Hep-Bp specifically cleaves the linkage between d-glucuronic acid (GlcA) and N-acetyl-d-glucosamine (GlcNAc) but not the linkage between 4-deoxy-α-L-threo-hex-4-enopyranosyluronic acid (deltaUA) and GlcNAc, and results in the presence of a minor resistant trisaccharide (GlcNAc-GlcA-GlcNAc).
33006278	8	84	theme	minor	1453:1457	arg1	trisaccharide					1469:1481	a minor resistant trisaccharide	1451:1481	a minor resistant trisaccharide (GlcNAc-GlcA-GlcNAc)	1451:1502	Hep-Bp specifically cleaves the linkage between d-glucuronic acid (GlcA) and N-acetyl-d-glucosamine (GlcNAc) but not the linkage between 4-deoxy-α-L-threo-hex-4-enopyranosyluronic acid (deltaUA) and GlcNAc, and results in the presence of a minor resistant trisaccharide (GlcNAc-GlcA-GlcNAc).
33006278	7	85	theme	gel	1182:1184	arg1	chromatography					1197:1210	gel permeation chromatography	1182:1210	gel permeation chromatography	1182:1210	The oligomers (degree of polymerization from 2 to 8, DP2-DP8) are completely digested with purified GST-tagged Hep-Bp and analyzed using gel permeation chromatography.
33006278	1	86	theme	potential	246:254	arg1	applications					256:267	potential applications	246:267	potential applications	246:267	Heparosan is a non-sulfated polysaccharide and potential applications include, chemoenzymatic synthesis of heparin and heparan sulfates.
32061697	6	0	theme	degradation	843:853	arg1	temperature					855:865	the degradation temperature	839:865	the degradation temperature	839:865	Thermal stability results showed enhancement in the degradation temperature, residual %, endothermic peak and enthalpy by the incorporation of CMF.
32061697	4	1	theme	filler	622:627	arg1	filler					622:627	filler	622:627	filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1	622:687	FTIR results confirmed the rich cellulose content in functional groups of filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1.
32061697	4	1	theme	filler	622:627	arg1	groups					612:617	functional groups	601:617	functional groups of filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1	601:687	FTIR results confirmed the rich cellulose content in functional groups of filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1.
32061697	6	2	theme	stability	799:807	arg1	results					809:815	Thermal stability results	791:815	Thermal stability results	791:815	Thermal stability results showed enhancement in the degradation temperature, residual %, endothermic peak and enthalpy by the incorporation of CMF.
32061697	1	3	theme	work	154:157	arg1	oil					166:168	this work peanut oil	149:168	this work peanut oil	149:168	In this work peanut oil cake extracted Cellulose Micro Filler (CMF) is used for the advancement of mechanical and thermal properties in natural fiber composites.
32061697	5	4	from	incorporation	739:751	arg1	composites					779:788	PF hybrid fiber composites	763:788	PF hybrid fiber composites	763:788	Mechanical results showed a positive impact with incorporation of CMF in PF hybrid fiber composites.
32061697	0	5	theme	mechanical	68:77	arg1	properties					91:100	mechanical and thermal properties	68:100	mechanical and thermal properties	68:100	Peanut oil cake-derived cellulose fiber: Extraction, application of mechanical and thermal properties in pineapple/flax natural fiber composites.
32061697	2	6	used	used	330:333	arg2	powder					319:324	This fiber powder	308:324	This fiber powder	308:324	This fiber powder was used in enhancing the applications of Pineapple (P)/Flax (F) natural fiber epoxy composites.
32061697	1	7	theme	peanut	159:164	arg1	oil					166:168	this work peanut oil	149:168	this work peanut oil	149:168	In this work peanut oil cake extracted Cellulose Micro Filler (CMF) is used for the advancement of mechanical and thermal properties in natural fiber composites.
32061697	7	8	theme	temperature	978:988	arg1	T50					990:992	the 30% PF combinations degradation temperature T50	942:992	the 30% PF combinations degradation temperature T50	942:992	In the 30% PF combinations degradation temperature T50, T70, T70 enhanced from 387.73-391.08°, 434.81-454.81° and 468.91-553.36° by the filler substitution.
32061697	0	9	theme	properties	91:100	arg1	application					53:63	application	53:63	application of mechanical and thermal properties in pineapple/flax natural fiber composites	53:143	Peanut oil cake-derived cellulose fiber: Extraction, application of mechanical and thermal properties in pineapple/flax natural fiber composites.
32061697	0	9	theme	properties	91:100	arg1	fiber					34:38	cellulose fiber	24:38	cellulose fiber	24:38	Peanut oil cake-derived cellulose fiber: Extraction, application of mechanical and thermal properties in pineapple/flax natural fiber composites.
32061697	6	10	from	enhancement	824:834	arg1	%					877:877	residual %	868:877	residual %	868:877	Thermal stability results showed enhancement in the degradation temperature, residual %, endothermic peak and enthalpy by the incorporation of CMF.
32061697	6	10	from	enhancement	824:834	arg1	peak					892:895	endothermic peak	880:895	endothermic peak	880:895	Thermal stability results showed enhancement in the degradation temperature, residual %, endothermic peak and enthalpy by the incorporation of CMF.
32061697	6	10	from	enhancement	824:834	arg1	temperature					855:865	the degradation temperature	839:865	the degradation temperature	839:865	Thermal stability results showed enhancement in the degradation temperature, residual %, endothermic peak and enthalpy by the incorporation of CMF.
32061697	6	10	from	enhancement	824:834	arg1	enthalpy					901:908	enthalpy	901:908	enthalpy	901:908	Thermal stability results showed enhancement in the degradation temperature, residual %, endothermic peak and enthalpy by the incorporation of CMF.
32061697	3	11	theme	improved	473:480	arg1	Crl					501:503	Crl	501:503	Crl	501:503	The X Ray Diffraction (XRD) results of CMF showed improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm.
32061697	3	11	theme	improved	473:480	arg1	Index					494:498	improved Crystalline Index	473:498	improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm	473:545	The X Ray Diffraction (XRD) results of CMF showed improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm.
32061697	9	12	theme	residual	1221:1228	arg1	percentage					1230:1239	residual percentage	1221:1239	residual percentage	1221:1239	The combination with 35% PF showed enhancement in degradation temperature, residual percentage, endothermic peak and enthalpy with filler addition up to 3%.
32061697	0	13	theme	thermal	83:89	arg1	properties					91:100	mechanical and thermal properties	68:100	mechanical and thermal properties	68:100	Peanut oil cake-derived cellulose fiber: Extraction, application of mechanical and thermal properties in pineapple/flax natural fiber composites.
32061697	4	14	with	filler	622:627	arg1	peaks					634:638	peaks	634:638	peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1	634:687	FTIR results confirmed the rich cellulose content in functional groups of filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1.
32061697	4	15	from	1428 cm-1	679:687	arg1	peaks					634:638	peaks	634:638	peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1	634:687	FTIR results confirmed the rich cellulose content in functional groups of filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1.
32061697	3	16	theme	Crystalline	482:492	arg1	Crl					501:503	Crl	501:503	Crl	501:503	The X Ray Diffraction (XRD) results of CMF showed improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm.
32061697	3	16	theme	Crystalline	482:492	arg1	Index					494:498	improved Crystalline Index	473:498	improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm	473:545	The X Ray Diffraction (XRD) results of CMF showed improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm.
32061697	1	17	theme	mechanical	245:254	arg1	properties					268:277	mechanical and thermal properties	245:277	mechanical and thermal properties	245:277	In this work peanut oil cake extracted Cellulose Micro Filler (CMF) is used for the advancement of mechanical and thermal properties in natural fiber composites.
32061697	0	18	theme	pineapple/flax	105:118	arg1	composites					134:143	pineapple/flax natural fiber composites	105:143	pineapple/flax natural fiber composites	105:143	Peanut oil cake-derived cellulose fiber: Extraction, application of mechanical and thermal properties in pineapple/flax natural fiber composites.
32061697	9	19	dep	3	1299:1299	arg1	to					1296:1297	to	1296:1297	to	1296:1297	The combination with 35% PF showed enhancement in degradation temperature, residual percentage, endothermic peak and enthalpy with filler addition up to 3%.
32061697	3	20	theme	CMF	462:464	arg1	results					451:457	The X Ray Diffraction (XRD) results	423:457	The X Ray Diffraction (XRD) results of CMF	423:464	The X Ray Diffraction (XRD) results of CMF showed improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm.
32061697	4	21	from	content	590:596	arg1	filler					622:627	filler	622:627	filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1	622:687	FTIR results confirmed the rich cellulose content in functional groups of filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1.
32061697	4	21	from	content	590:596	arg1	groups					612:617	functional groups	601:617	functional groups of filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1	601:687	FTIR results confirmed the rich cellulose content in functional groups of filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1.
32061697	4	22	theme	cellulose	580:588	arg1	content					590:596	the rich cellulose content	571:596	the rich cellulose content in functional groups of filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1	571:687	FTIR results confirmed the rich cellulose content in functional groups of filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1.
32061697	5	23	theme	positive	718:725	arg1	impact					727:732	a positive impact	716:732	a positive impact	716:732	Mechanical results showed a positive impact with incorporation of CMF in PF hybrid fiber composites.
32061697	9	24	theme	endothermic	1242:1252	arg1	peak					1254:1257	endothermic peak	1242:1257	endothermic peak	1242:1257	The combination with 35% PF showed enhancement in degradation temperature, residual percentage, endothermic peak and enthalpy with filler addition up to 3%.
32061697	4	25	from	1370 cm-1	665:673	arg1	peaks					634:638	peaks	634:638	peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1	634:687	FTIR results confirmed the rich cellulose content in functional groups of filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1.
32061697	2	26	theme	natural	391:397	arg1	composites					411:420	Pineapple (P)/Flax (F) natural fiber epoxy composites	368:420	Pineapple (P)/Flax (F) natural fiber epoxy composites	368:420	This fiber powder was used in enhancing the applications of Pineapple (P)/Flax (F) natural fiber epoxy composites.
32061697	1	27	theme	cake	170:173	arg1	Filler					201:206	cake extracted Cellulose Micro Filler	170:206	cake extracted Cellulose Micro Filler (CMF)	170:212	In this work peanut oil cake extracted Cellulose Micro Filler (CMF) is used for the advancement of mechanical and thermal properties in natural fiber composites.
32061697	1	27	theme	cake	170:173	arg1	CMF					209:211	CMF	209:211	CMF	209:211	In this work peanut oil cake extracted Cellulose Micro Filler (CMF) is used for the advancement of mechanical and thermal properties in natural fiber composites.
32061697	2	28	theme	fiber	399:403	arg1	composites					411:420	Pineapple (P)/Flax (F) natural fiber epoxy composites	368:420	Pineapple (P)/Flax (F) natural fiber epoxy composites	368:420	This fiber powder was used in enhancing the applications of Pineapple (P)/Flax (F) natural fiber epoxy composites.
32061697	5	29	theme	Mechanical	690:699	arg1	results					701:707	Mechanical results	690:707	Mechanical results	690:707	Mechanical results showed a positive impact with incorporation of CMF in PF hybrid fiber composites.
32061697	1	30	theme	extracted	175:183	arg1	Filler					201:206	cake extracted Cellulose Micro Filler	170:206	cake extracted Cellulose Micro Filler (CMF)	170:212	In this work peanut oil cake extracted Cellulose Micro Filler (CMF) is used for the advancement of mechanical and thermal properties in natural fiber composites.
32061697	1	30	theme	extracted	175:183	arg1	CMF					209:211	CMF	209:211	CMF	209:211	In this work peanut oil cake extracted Cellulose Micro Filler (CMF) is used for the advancement of mechanical and thermal properties in natural fiber composites.
32061697	0	31	theme	Peanut	0:5	arg1	oil					7:9	Peanut oil	0:9	Peanut oil	0:9	Peanut oil cake-derived cellulose fiber: Extraction, application of mechanical and thermal properties in pineapple/flax natural fiber composites.
32061697	4	32	from	1162 cm-1	654:662	arg1	peaks					634:638	peaks	634:638	peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1	634:687	FTIR results confirmed the rich cellulose content in functional groups of filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1.
32061697	1	33	theme	Cellulose	185:193	arg1	Filler					201:206	cake extracted Cellulose Micro Filler	170:206	cake extracted Cellulose Micro Filler (CMF)	170:212	In this work peanut oil cake extracted Cellulose Micro Filler (CMF) is used for the advancement of mechanical and thermal properties in natural fiber composites.
32061697	1	33	theme	Cellulose	185:193	arg1	CMF					209:211	CMF	209:211	CMF	209:211	In this work peanut oil cake extracted Cellulose Micro Filler (CMF) is used for the advancement of mechanical and thermal properties in natural fiber composites.
32061697	0	34	theme	cellulose	24:32	arg1	application					53:63	application	53:63	application of mechanical and thermal properties in pineapple/flax natural fiber composites	53:143	Peanut oil cake-derived cellulose fiber: Extraction, application of mechanical and thermal properties in pineapple/flax natural fiber composites.
32061697	0	34	theme	cellulose	24:32	arg1	fiber					34:38	cellulose fiber	24:38	cellulose fiber	24:38	Peanut oil cake-derived cellulose fiber: Extraction, application of mechanical and thermal properties in pineapple/flax natural fiber composites.
32061697	1	35	theme	thermal	260:266	arg1	properties					268:277	mechanical and thermal properties	245:277	mechanical and thermal properties	245:277	In this work peanut oil cake extracted Cellulose Micro Filler (CMF) is used for the advancement of mechanical and thermal properties in natural fiber composites.
32061697	0	36	theme	fiber	128:132	arg1	composites					134:143	pineapple/flax natural fiber composites	105:143	pineapple/flax natural fiber composites	105:143	Peanut oil cake-derived cellulose fiber: Extraction, application of mechanical and thermal properties in pineapple/flax natural fiber composites.
32061697	3	37	theme	70.25°	509:514	arg1	Crl					501:503	Crl	501:503	Crl	501:503	The X Ray Diffraction (XRD) results of CMF showed improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm.
32061697	3	37	theme	70.25°	509:514	arg1	Index					494:498	improved Crystalline Index	473:498	improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm	473:545	The X Ray Diffraction (XRD) results of CMF showed improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm.
32061697	1	38	theme	Micro	195:199	arg1	Filler					201:206	cake extracted Cellulose Micro Filler	170:206	cake extracted Cellulose Micro Filler (CMF)	170:212	In this work peanut oil cake extracted Cellulose Micro Filler (CMF) is used for the advancement of mechanical and thermal properties in natural fiber composites.
32061697	1	38	theme	Micro	195:199	arg1	CMF					209:211	CMF	209:211	CMF	209:211	In this work peanut oil cake extracted Cellulose Micro Filler (CMF) is used for the advancement of mechanical and thermal properties in natural fiber composites.
32061697	1	39	theme	properties	268:277	arg1	advancement					230:240	the advancement	226:240	the advancement of mechanical and thermal properties in natural fiber composites	226:305	In this work peanut oil cake extracted Cellulose Micro Filler (CMF) is used for the advancement of mechanical and thermal properties in natural fiber composites.
32061697	0	40	theme	natural	120:126	arg1	composites					134:143	pineapple/flax natural fiber composites	105:143	pineapple/flax natural fiber composites	105:143	Peanut oil cake-derived cellulose fiber: Extraction, application of mechanical and thermal properties in pineapple/flax natural fiber composites.
32061697	6	41	theme	Thermal	791:797	arg1	stability					799:807	Thermal stability	791:807	Thermal stability results	791:815	Thermal stability results showed enhancement in the degradation temperature, residual %, endothermic peak and enthalpy by the incorporation of CMF.
32061697	9	42	theme	filler	1277:1282	arg1	addition					1284:1291	filler addition	1277:1291	filler addition up to 3%	1277:1300	The combination with 35% PF showed enhancement in degradation temperature, residual percentage, endothermic peak and enthalpy with filler addition up to 3%.
32061697	9	43	with	combination	1150:1160	arg1	PF					1171:1172	35% PF	1167:1172	35% PF	1167:1172	The combination with 35% PF showed enhancement in degradation temperature, residual percentage, endothermic peak and enthalpy with filler addition up to 3%.
32061697	4	44	theme	FTIR	548:551	arg1	results					553:559	FTIR results	548:559	FTIR results	548:559	FTIR results confirmed the rich cellulose content in functional groups of filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1.
32061697	6	45	theme	endothermic	880:890	arg1	peak					892:895	endothermic peak	880:895	endothermic peak	880:895	Thermal stability results showed enhancement in the degradation temperature, residual %, endothermic peak and enthalpy by the incorporation of CMF.
32061697	7	46	theme	degradation	966:976	arg1	T50					990:992	the 30% PF combinations degradation temperature T50	942:992	the 30% PF combinations degradation temperature T50	942:992	In the 30% PF combinations degradation temperature T50, T70, T70 enhanced from 387.73-391.08°, 434.81-454.81° and 468.91-553.36° by the filler substitution.
32061697	5	47	theme	PF	763:764	arg1	composites					779:788	PF hybrid fiber composites	763:788	PF hybrid fiber composites	763:788	Mechanical results showed a positive impact with incorporation of CMF in PF hybrid fiber composites.
32061697	5	48	theme	fiber	773:777	arg1	composites					779:788	PF hybrid fiber composites	763:788	PF hybrid fiber composites	763:788	Mechanical results showed a positive impact with incorporation of CMF in PF hybrid fiber composites.
32061697	1	49	theme	natural	282:288	arg1	composites					296:305	natural fiber composites	282:305	natural fiber composites	282:305	In this work peanut oil cake extracted Cellulose Micro Filler (CMF) is used for the advancement of mechanical and thermal properties in natural fiber composites.
32061697	1	50	from	advancement	230:240	arg1	composites					296:305	natural fiber composites	282:305	natural fiber composites	282:305	In this work peanut oil cake extracted Cellulose Micro Filler (CMF) is used for the advancement of mechanical and thermal properties in natural fiber composites.
32061697	9	51	theme	35	1167:1168	arg1	%					1169:1169	%	1169:1169	%	1169:1169	The combination with 35% PF showed enhancement in degradation temperature, residual percentage, endothermic peak and enthalpy with filler addition up to 3%.
32061697	3	52	theme	crystalline	520:530	arg1	size					532:535	crystalline size	520:535	crystalline size	520:535	The X Ray Diffraction (XRD) results of CMF showed improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm.
32061697	5	53	theme	hybrid	766:771	arg1	composites					779:788	PF hybrid fiber composites	763:788	PF hybrid fiber composites	763:788	Mechanical results showed a positive impact with incorporation of CMF in PF hybrid fiber composites.
32061697	2	54	theme	composites	411:420	arg1	applications					352:363	the applications	348:363	the applications of Pineapple (P)/Flax (F) natural fiber epoxy composites	348:420	This fiber powder was used in enhancing the applications of Pineapple (P)/Flax (F) natural fiber epoxy composites.
32061697	1	55	theme	fiber	290:294	arg1	composites					296:305	natural fiber composites	282:305	natural fiber composites	282:305	In this work peanut oil cake extracted Cellulose Micro Filler (CMF) is used for the advancement of mechanical and thermal properties in natural fiber composites.
32061697	7	56	theme	PF	950:951	arg1	T50					990:992	the 30% PF combinations degradation temperature T50	942:992	the 30% PF combinations degradation temperature T50	942:992	In the 30% PF combinations degradation temperature T50, T70, T70 enhanced from 387.73-391.08°, 434.81-454.81° and 468.91-553.36° by the filler substitution.
32061697	0	57	from	application	53:63	arg1	composites					134:143	pineapple/flax natural fiber composites	105:143	pineapple/flax natural fiber composites	105:143	Peanut oil cake-derived cellulose fiber: Extraction, application of mechanical and thermal properties in pineapple/flax natural fiber composites.
32061697	9	58	dep	addition	1284:1291	arg1	%					1300:1300	%	1300:1300	%	1300:1300	The combination with 35% PF showed enhancement in degradation temperature, residual percentage, endothermic peak and enthalpy with filler addition up to 3%.
32061697	3	59	theme	Diffraction	433:443	arg1	results					451:457	The X Ray Diffraction (XRD) results	423:457	The X Ray Diffraction (XRD) results of CMF	423:464	The X Ray Diffraction (XRD) results of CMF showed improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm.
32061697	2	60	theme	epoxy	405:409	arg1	composites					411:420	Pineapple (P)/Flax (F) natural fiber epoxy composites	368:420	Pineapple (P)/Flax (F) natural fiber epoxy composites	368:420	This fiber powder was used in enhancing the applications of Pineapple (P)/Flax (F) natural fiber epoxy composites.
32061697	6	61	theme	residual	868:875	arg1	%					877:877	residual %	868:877	residual %	868:877	Thermal stability results showed enhancement in the degradation temperature, residual %, endothermic peak and enthalpy by the incorporation of CMF.
32061697	7	62	theme	combinations	953:964	arg1	T50					990:992	the 30% PF combinations degradation temperature T50	942:992	the 30% PF combinations degradation temperature T50	942:992	In the 30% PF combinations degradation temperature T50, T70, T70 enhanced from 387.73-391.08°, 434.81-454.81° and 468.91-553.36° by the filler substitution.
32061697	9	63	theme	%	1169:1169	arg1	PF					1171:1172	35% PF	1167:1172	35% PF	1167:1172	The combination with 35% PF showed enhancement in degradation temperature, residual percentage, endothermic peak and enthalpy with filler addition up to 3%.
32061697	0	64	dep	fiber	34:38	arg1	Extraction					41:50	Extraction	41:50	Extraction	41:50	Peanut oil cake-derived cellulose fiber: Extraction, application of mechanical and thermal properties in pineapple/flax natural fiber composites.
32061697	9	65	dep	%	1300:1300	arg1	3					1299:1299	3	1299:1299	3	1299:1299	The combination with 35% PF showed enhancement in degradation temperature, residual percentage, endothermic peak and enthalpy with filler addition up to 3%.
32061697	4	66	from	1058 cm-1	643:651	arg1	peaks					634:638	peaks	634:638	peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1	634:687	FTIR results confirmed the rich cellulose content in functional groups of filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1.
32061697	7	67	theme	filler	1075:1080	arg1	substitution					1082:1093	the filler substitution	1071:1093	the filler substitution	1071:1093	In the 30% PF combinations degradation temperature T50, T70, T70 enhanced from 387.73-391.08°, 434.81-454.81° and 468.91-553.36° by the filler substitution.
32061697	7	68	theme	30	946:947	arg1	%					948:948	%	948:948	%	948:948	In the 30% PF combinations degradation temperature T50, T70, T70 enhanced from 387.73-391.08°, 434.81-454.81° and 468.91-553.36° by the filler substitution.
32061697	1	69	used	used	217:220	arg2	CMF					209:211	CMF	209:211	CMF	209:211	In this work peanut oil cake extracted Cellulose Micro Filler (CMF) is used for the advancement of mechanical and thermal properties in natural fiber composites.
32061697	1	69	used	used	217:220	arg2	Filler					201:206	cake extracted Cellulose Micro Filler	170:206	cake extracted Cellulose Micro Filler (CMF)	170:212	In this work peanut oil cake extracted Cellulose Micro Filler (CMF) is used for the advancement of mechanical and thermal properties in natural fiber composites.
32061697	3	70	theme	5.5 nm	540:545	arg1	70.25°					509:514	70.25°	509:514	70.25°	509:514	The X Ray Diffraction (XRD) results of CMF showed improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm.
32061697	3	70	theme	5.5 nm	540:545	arg1	size					532:535	crystalline size	520:535	crystalline size	520:535	The X Ray Diffraction (XRD) results of CMF showed improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm.
32061697	2	71	theme	fiber	313:317	arg1	powder					319:324	This fiber powder	308:324	This fiber powder	308:324	This fiber powder was used in enhancing the applications of Pineapple (P)/Flax (F) natural fiber epoxy composites.
32061697	7	72	theme	%	948:948	arg1	T50					990:992	the 30% PF combinations degradation temperature T50	942:992	the 30% PF combinations degradation temperature T50	942:992	In the 30% PF combinations degradation temperature T50, T70, T70 enhanced from 387.73-391.08°, 434.81-454.81° and 468.91-553.36° by the filler substitution.
32061697	8	73	theme	residual	1106:1113	arg1	%					1115:1115	residual %	1106:1115	residual %	1106:1115	Similarly residual % increased from 17.69-24.35%.
32061697	3	74	theme	Ray	429:431	arg1	Diffraction					433:443	The X Ray Diffraction	423:443	The X Ray Diffraction (XRD) results of CMF	423:464	The X Ray Diffraction (XRD) results of CMF showed improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm.
32061697	3	74	theme	Ray	429:431	arg1	XRD					446:448	XRD	446:448	XRD	446:448	The X Ray Diffraction (XRD) results of CMF showed improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm.
32061697	3	75	theme	X	427:427	arg1	Diffraction					433:443	The X Ray Diffraction	423:443	The X Ray Diffraction (XRD) results of CMF	423:464	The X Ray Diffraction (XRD) results of CMF showed improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm.
32061697	3	75	theme	X	427:427	arg1	XRD					446:448	XRD	446:448	XRD	446:448	The X Ray Diffraction (XRD) results of CMF showed improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm.
32061697	9	76	from	enhancement	1181:1191	arg1	temperature					1208:1218	degradation temperature	1196:1218	degradation temperature	1196:1218	The combination with 35% PF showed enhancement in degradation temperature, residual percentage, endothermic peak and enthalpy with filler addition up to 3%.
32061697	9	76	from	enhancement	1181:1191	arg1	enthalpy					1263:1270	enthalpy	1263:1270	enthalpy	1263:1270	The combination with 35% PF showed enhancement in degradation temperature, residual percentage, endothermic peak and enthalpy with filler addition up to 3%.
32061697	9	76	from	enhancement	1181:1191	arg1	percentage					1230:1239	residual percentage	1221:1239	residual percentage	1221:1239	The combination with 35% PF showed enhancement in degradation temperature, residual percentage, endothermic peak and enthalpy with filler addition up to 3%.
32061697	9	76	from	enhancement	1181:1191	arg1	peak					1254:1257	endothermic peak	1242:1257	endothermic peak	1242:1257	The combination with 35% PF showed enhancement in degradation temperature, residual percentage, endothermic peak and enthalpy with filler addition up to 3%.
32061697	3	77	theme	size	532:535	arg1	Crl					501:503	Crl	501:503	Crl	501:503	The X Ray Diffraction (XRD) results of CMF showed improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm.
32061697	3	77	theme	size	532:535	arg1	Index					494:498	improved Crystalline Index	473:498	improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm	473:545	The X Ray Diffraction (XRD) results of CMF showed improved Crystalline Index (Crl) of 70.25° and crystalline size of 5.5 nm.
32061697	5	78	theme	CMF	756:758	arg1	incorporation					739:751	incorporation	739:751	incorporation of CMF in PF hybrid fiber composites	739:788	Mechanical results showed a positive impact with incorporation of CMF in PF hybrid fiber composites.
32061697	4	79	theme	rich	575:578	arg1	content					590:596	the rich cellulose content	571:596	the rich cellulose content in functional groups of filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1	571:687	FTIR results confirmed the rich cellulose content in functional groups of filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1.
32061697	9	80	theme	degradation	1196:1206	arg1	temperature					1208:1218	degradation temperature	1196:1218	degradation temperature	1196:1218	The combination with 35% PF showed enhancement in degradation temperature, residual percentage, endothermic peak and enthalpy with filler addition up to 3%.
32061697	4	81	theme	functional	601:610	arg1	filler					622:627	filler	622:627	filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1	622:687	FTIR results confirmed the rich cellulose content in functional groups of filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1.
32061697	4	81	theme	functional	601:610	arg1	groups					612:617	functional groups	601:617	functional groups of filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1	601:687	FTIR results confirmed the rich cellulose content in functional groups of filler with peaks at 1058 cm-1, 1162 cm-1, 1370 cm-1 and 1428 cm-1.
32061697	6	82	theme	CMF	934:936	arg1	incorporation					917:929	the incorporation	913:929	the incorporation of CMF	913:936	Thermal stability results showed enhancement in the degradation temperature, residual %, endothermic peak and enthalpy by the incorporation of CMF.
34687818	7	0	theme	water	1187:1191	arg1	sorption					1193:1200	water sorption	1187:1200	water sorption	1187:1200	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	7	1	theme	amorphous	1105:1113	arg1	73 °C					1120:1124	73 °C	1120:1124	73 °C	1120:1124	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	7	1	theme	amorphous	1105:1113	arg1	GLB					1115:1117	neat amorphous GLB	1100:1117	neat amorphous GLB (73 °C)	1100:1125	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	7	2	theme	HPMC	983:986	arg1	diffusion					970:978	the diffusion	966:978	the diffusion of HPMC from amorphous GLB nanoparticles	966:1019	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	3	3	theme	aqueous	508:514	arg1	solution					516:523	an aqueous solution	505:523	an aqueous solution containing hydroxypropyl methylcellulose (HPMC) to obtain nano-A suspensions	505:600	In method A, N,N-dimethylformamide (DMF) solution of GLB was added to an aqueous solution containing hydroxypropyl methylcellulose (HPMC) to obtain nano-A suspensions.
34687818	1	4	theme	nanoparticles	269:281	arg1	properties					240:249	the physicochemical properties	220:249	the physicochemical properties of amorphous drug nanoparticles	220:281	The present study focuses on the effect of the preparation temperature on the physicochemical properties of amorphous drug nanoparticles to clarify their formation mechanism.
34687818	8	5	theme	increased	1292:1300	arg1	HPMC					1302:1305	increased HPMC	1292:1305	increased HPMC in the nanoparticles	1292:1326	The physical stability of amorphous GLB nanoparticles was improved with increased HPMC in the nanoparticles.
34687818	7	6	theme	GLB	1115:1117	arg1	Tg					1093:1094	Tg	1093:1094	Tg	1093:1094	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	7	6	theme	GLB	1115:1117	arg1	temperature					1080:1090	the glass transition temperature	1059:1090	the glass transition temperature (Tg) of neat amorphous GLB (73 °C)	1059:1125	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	3	7	contain	containing	525:534	arg1	solution					516:523	an aqueous solution	505:523	an aqueous solution containing hydroxypropyl methylcellulose (HPMC) to obtain nano-A suspensions	505:600	In method A, N,N-dimethylformamide (DMF) solution of GLB was added to an aqueous solution containing hydroxypropyl methylcellulose (HPMC) to obtain nano-A suspensions.
34687818	3	7	contain	containing	525:534	arg2	HPMC					567:570	HPMC	567:570	HPMC	567:570	In method A, N,N-dimethylformamide (DMF) solution of GLB was added to an aqueous solution containing hydroxypropyl methylcellulose (HPMC) to obtain nano-A suspensions.
34687818	3	7	contain	containing	525:534	arg2	methylcellulose					550:564	hydroxypropyl methylcellulose	536:564	hydroxypropyl methylcellulose (HPMC) to obtain nano-A suspensions	536:600	In method A, N,N-dimethylformamide (DMF) solution of GLB was added to an aqueous solution containing hydroxypropyl methylcellulose (HPMC) to obtain nano-A suspensions.
34687818	2	8	theme	Amorphous	321:329	arg1	GLB					346:348	GLB	346:348	GLB	346:348	Amorphous glibenclamide (GLB) nanoparticles were prepared at 4-40 °C using two antisolvent precipitation methods.
34687818	2	8	theme	Amorphous	321:329	arg1	glibenclamide					331:343	Amorphous glibenclamide	321:343	Amorphous glibenclamide (GLB) nanoparticles	321:363	Amorphous glibenclamide (GLB) nanoparticles were prepared at 4-40 °C using two antisolvent precipitation methods.
34687818	9	9	theme	antisolvent-saturated	1400:1420	arg1	nanoparticles					1437:1449	the antisolvent-saturated amorphous drug nanoparticles	1396:1449	the antisolvent-saturated amorphous drug nanoparticles	1396:1449	Thus, setting the preparation temperature by considering the Tg of the antisolvent-saturated amorphous drug nanoparticles is essential to develop stable amorphous drug nanoparticles.
34687818	7	10	theme	neat	1100:1103	arg1	73 °C					1120:1124	73 °C	1120:1124	73 °C	1120:1124	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	7	10	theme	neat	1100:1103	arg1	GLB					1115:1117	neat amorphous GLB	1100:1117	neat amorphous GLB (73 °C)	1100:1125	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	7	11	theme	cores	950:954	arg1	nature					923:928	The glassy nature	912:928	The glassy nature of the nanoparticle cores	912:954	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	7	12	theme	GLB	1215:1217	arg1	nanosizing					1172:1181	nanosizing	1172:1181	nanosizing	1172:1181	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	7	12	theme	GLB	1215:1217	arg1	sorption					1193:1200	water sorption	1187:1200	water sorption	1187:1200	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	4	13	theme	method	606:611	arg1	B					613:613	method B	606:613	method B	606:613	In method B, nano-B suspensions were obtained by adding DMF solution containing both GLB and HPMC into water.
34687818	0	14	theme	preparation	121:131	arg1	temperature					133:143	the preparation temperature	117:143	the preparation temperature	117:143	Formation mechanism of amorphous drug nanoparticles using the antisolvent precipitation method elucidated by varying the preparation temperature.
34687818	7	15	from	nanoparticles	1007:1019	arg1	diffusion					970:978	the diffusion	966:978	the diffusion of HPMC from amorphous GLB nanoparticles	966:1019	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	5	16	theme	similar	788:794	arg1	compositions					801:812	similar HPMC compositions	788:812	similar HPMC compositions	788:812	When the preparation temperature was above 25 °C, nano-A and nano-B showed similar HPMC compositions.
34687818	9	17	theme	amorphous	1422:1430	arg1	nanoparticles					1437:1449	the antisolvent-saturated amorphous drug nanoparticles	1396:1449	the antisolvent-saturated amorphous drug nanoparticles	1396:1449	Thus, setting the preparation temperature by considering the Tg of the antisolvent-saturated amorphous drug nanoparticles is essential to develop stable amorphous drug nanoparticles.
34687818	9	18	theme	nanoparticles	1437:1449	arg1	Tg					1390:1391	the Tg	1386:1391	the Tg of the antisolvent-saturated amorphous drug nanoparticles	1386:1449	Thus, setting the preparation temperature by considering the Tg of the antisolvent-saturated amorphous drug nanoparticles is essential to develop stable amorphous drug nanoparticles.
34687818	7	19	theme	nanoparticle	937:948	arg1	cores					950:954	the nanoparticle cores	933:954	the nanoparticle cores	933:954	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	3	20	theme	nano-A	583:588	arg1	suspensions					590:600	nano-A suspensions	583:600	nano-A suspensions	583:600	In method A, N,N-dimethylformamide (DMF) solution of GLB was added to an aqueous solution containing hydroxypropyl methylcellulose (HPMC) to obtain nano-A suspensions.
34687818	8	21	theme	physical	1224:1231	arg1	stability					1233:1241	The physical stability	1220:1241	The physical stability of amorphous GLB nanoparticles	1220:1272	The physical stability of amorphous GLB nanoparticles was improved with increased HPMC in the nanoparticles.
34687818	0	22	theme	Formation	0:8	arg1	mechanism					10:18	Formation mechanism	0:18	Formation mechanism of amorphous drug	0:36	Formation mechanism of amorphous drug nanoparticles using the antisolvent precipitation method elucidated by varying the preparation temperature.
34687818	1	23	theme	formation	300:308	arg1	mechanism					310:318	their formation mechanism	294:318	their formation mechanism	294:318	The present study focuses on the effect of the preparation temperature on the physicochemical properties of amorphous drug nanoparticles to clarify their formation mechanism.
34687818	8	24	from	HPMC	1302:1305	arg1	nanoparticles					1314:1326	the nanoparticles	1310:1326	the nanoparticles	1310:1326	The physical stability of amorphous GLB nanoparticles was improved with increased HPMC in the nanoparticles.
34687818	2	25	theme	precipitation	412:424	arg1	methods					426:432	two antisolvent precipitation methods	396:432	two antisolvent precipitation methods	396:432	Amorphous glibenclamide (GLB) nanoparticles were prepared at 4-40 °C using two antisolvent precipitation methods.
34687818	0	26	theme	amorphous	23:31	arg1	drug					33:36	amorphous drug	23:36	amorphous drug	23:36	Formation mechanism of amorphous drug nanoparticles using the antisolvent precipitation method elucidated by varying the preparation temperature.
34687818	9	27	theme	amorphous	1482:1490	arg1	nanoparticles					1497:1509	stable amorphous drug nanoparticles	1475:1509	stable amorphous drug nanoparticles	1475:1509	Thus, setting the preparation temperature by considering the Tg of the antisolvent-saturated amorphous drug nanoparticles is essential to develop stable amorphous drug nanoparticles.
34687818	7	28	theme	glass	1063:1067	arg1	transition					1069:1078	the glass transition	1059:1078	the glass transition temperature (Tg) of neat amorphous GLB (73 °C)	1059:1125	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	9	29	theme	drug	1432:1435	arg1	nanoparticles					1437:1449	the antisolvent-saturated amorphous drug nanoparticles	1396:1449	the antisolvent-saturated amorphous drug nanoparticles	1396:1449	Thus, setting the preparation temperature by considering the Tg of the antisolvent-saturated amorphous drug nanoparticles is essential to develop stable amorphous drug nanoparticles.
34687818	5	30	theme	HPMC	796:799	arg1	compositions					801:812	similar HPMC compositions	788:812	similar HPMC compositions	788:812	When the preparation temperature was above 25 °C, nano-A and nano-B showed similar HPMC compositions.
34687818	1	31	theme	temperature	205:215	arg1	effect					179:184	the effect	175:184	the effect of the preparation temperature on the physicochemical properties of amorphous drug nanoparticles	175:281	The present study focuses on the effect of the preparation temperature on the physicochemical properties of amorphous drug nanoparticles to clarify their formation mechanism.
34687818	7	32	theme	transition	1069:1078	arg1	Tg					1093:1094	Tg	1093:1094	Tg	1093:1094	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	7	32	theme	transition	1069:1078	arg1	temperature					1080:1090	the glass transition temperature	1059:1090	the glass transition temperature (Tg) of neat amorphous GLB (73 °C)	1059:1125	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	2	33	theme	glibenclamide	331:343	arg1	nanoparticles					351:363	Amorphous glibenclamide (GLB) nanoparticles	321:363	Amorphous glibenclamide (GLB) nanoparticles	321:363	Amorphous glibenclamide (GLB) nanoparticles were prepared at 4-40 °C using two antisolvent precipitation methods.
34687818	3	34	theme	GLB	488:490	arg1	solution					476:483	solution	476:483	solution of GLB	476:490	In method A, N,N-dimethylformamide (DMF) solution of GLB was added to an aqueous solution containing hydroxypropyl methylcellulose (HPMC) to obtain nano-A suspensions.
34687818	8	35	theme	GLB	1256:1258	arg1	nanoparticles					1260:1272	amorphous GLB nanoparticles	1246:1272	amorphous GLB nanoparticles	1246:1272	The physical stability of amorphous GLB nanoparticles was improved with increased HPMC in the nanoparticles.
34687818	1	36	theme	preparation	193:203	arg1	temperature					205:215	the preparation temperature	189:215	the preparation temperature	189:215	The present study focuses on the effect of the preparation temperature on the physicochemical properties of amorphous drug nanoparticles to clarify their formation mechanism.
34687818	4	37	theme	DMF	659:661	arg1	solution					663:670	DMF solution	659:670	DMF solution containing both GLB and HPMC into water	659:710	In method B, nano-B suspensions were obtained by adding DMF solution containing both GLB and HPMC into water.
34687818	0	38	theme	drug	33:36	arg1	mechanism					10:18	Formation mechanism	0:18	Formation mechanism of amorphous drug	0:36	Formation mechanism of amorphous drug nanoparticles using the antisolvent precipitation method elucidated by varying the preparation temperature.
34687818	5	39	theme	preparation	722:732	arg1	temperature					734:744	the preparation temperature	718:744	the preparation temperature	718:744	When the preparation temperature was above 25 °C, nano-A and nano-B showed similar HPMC compositions.
34687818	6	40	theme	HPMC	859:862	arg1	HPMC					859:862	HPMC	859:862	HPMC	859:862	However, nano-B contained a large amount of HPMC compared to nano-A at temperatures below 20 °C.
34687818	6	40	theme	HPMC	859:862	arg1	amount					849:854	a large amount	841:854	a large amount of HPMC	841:862	However, nano-B contained a large amount of HPMC compared to nano-A at temperatures below 20 °C.
34687818	1	41	theme	physicochemical	224:238	arg1	properties					240:249	the physicochemical properties	220:249	the physicochemical properties of amorphous drug nanoparticles	220:281	The present study focuses on the effect of the preparation temperature on the physicochemical properties of amorphous drug nanoparticles to clarify their formation mechanism.
34687818	3	42	theme	method	438:443	arg1	A					445:445	method A	438:445	method A	438:445	In method A, N,N-dimethylformamide (DMF) solution of GLB was added to an aqueous solution containing hydroxypropyl methylcellulose (HPMC) to obtain nano-A suspensions.
34687818	3	42	theme	method	438:443	arg1	N-dimethylformamide					450:468	N-dimethylformamide	450:468	N-dimethylformamide (DMF)	450:474	In method A, N,N-dimethylformamide (DMF) solution of GLB was added to an aqueous solution containing hydroxypropyl methylcellulose (HPMC) to obtain nano-A suspensions.
34687818	3	42	theme	method	438:443	arg1	N					448:448	N	448:448	N	448:448	In method A, N,N-dimethylformamide (DMF) solution of GLB was added to an aqueous solution containing hydroxypropyl methylcellulose (HPMC) to obtain nano-A suspensions.
34687818	7	43	theme	amorphous	1205:1213	arg1	GLB					1215:1217	amorphous GLB	1205:1217	amorphous GLB	1205:1217	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	9	44	theme	stable	1475:1480	arg1	nanoparticles					1497:1509	stable amorphous drug nanoparticles	1475:1509	stable amorphous drug nanoparticles	1475:1509	Thus, setting the preparation temperature by considering the Tg of the antisolvent-saturated amorphous drug nanoparticles is essential to develop stable amorphous drug nanoparticles.
34687818	7	45	dep	nanosizing	1172:1181	arg1	the					1168:1170	the	1168:1170	the	1168:1170	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	9	46	theme	drug	1492:1495	arg1	nanoparticles					1497:1509	stable amorphous drug nanoparticles	1475:1509	stable amorphous drug nanoparticles	1475:1509	Thus, setting the preparation temperature by considering the Tg of the antisolvent-saturated amorphous drug nanoparticles is essential to develop stable amorphous drug nanoparticles.
34687818	9	47	theme	preparation	1347:1357	arg1	temperature					1359:1369	the preparation temperature	1343:1369	the preparation temperature	1343:1369	Thus, setting the preparation temperature by considering the Tg of the antisolvent-saturated amorphous drug nanoparticles is essential to develop stable amorphous drug nanoparticles.
34687818	7	48	theme	GLB	1003:1005	arg1	nanoparticles					1007:1019	amorphous GLB nanoparticles	993:1019	amorphous GLB nanoparticles	993:1019	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	8	49	theme	nanoparticles	1260:1272	arg1	stability					1233:1241	The physical stability	1220:1241	The physical stability of amorphous GLB nanoparticles	1220:1272	The physical stability of amorphous GLB nanoparticles was improved with increased HPMC in the nanoparticles.
34687818	7	50	theme	aqueous	1028:1034	arg1	phase					1036:1040	the aqueous phase	1024:1040	the aqueous phase	1024:1040	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	6	51	theme	large	843:847	arg1	HPMC					859:862	HPMC	859:862	HPMC	859:862	However, nano-B contained a large amount of HPMC compared to nano-A at temperatures below 20 °C.
34687818	6	51	theme	large	843:847	arg1	amount					849:854	a large amount	841:854	a large amount of HPMC	841:862	However, nano-B contained a large amount of HPMC compared to nano-A at temperatures below 20 °C.
34687818	7	52	theme	glassy	916:921	arg1	nature					923:928	The glassy nature	912:928	The glassy nature of the nanoparticle cores	912:954	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	4	53	theme	nano-B	616:621	arg1	suspensions					623:633	nano-B suspensions	616:633	nano-B suspensions	616:633	In method B, nano-B suspensions were obtained by adding DMF solution containing both GLB and HPMC into water.
34687818	6	54	contain	contained	831:839	arg1	temperatures					886:897	temperatures	886:897	temperatures below 20 °C	886:909	However, nano-B contained a large amount of HPMC compared to nano-A at temperatures below 20 °C.
34687818	6	54	contain	contained	831:839	arg2	nano-B					824:829	nano-B	824:829	nano-B	824:829	However, nano-B contained a large amount of HPMC compared to nano-A at temperatures below 20 °C.
34687818	6	54	contain	contained	831:839	arg2	HPMC					859:862	HPMC	859:862	HPMC	859:862	However, nano-B contained a large amount of HPMC compared to nano-A at temperatures below 20 °C.
34687818	6	54	contain	contained	831:839	arg2	amount					849:854	a large amount	841:854	a large amount of HPMC	841:862	However, nano-B contained a large amount of HPMC compared to nano-A at temperatures below 20 °C.
34687818	6	54	contain	contained	831:839	arg1	nano-B					824:829	nano-B	824:829	nano-B	824:829	However, nano-B contained a large amount of HPMC compared to nano-A at temperatures below 20 °C.
34687818	1	55	theme	present	150:156	arg1	study					158:162	The present study	146:162	The present study	146:162	The present study focuses on the effect of the preparation temperature on the physicochemical properties of amorphous drug nanoparticles to clarify their formation mechanism.
34687818	0	56	theme	precipitation	74:86	arg1	method					88:93	the antisolvent precipitation method	58:93	the antisolvent precipitation method elucidated by varying the preparation temperature	58:143	Formation mechanism of amorphous drug nanoparticles using the antisolvent precipitation method elucidated by varying the preparation temperature.
34687818	4	57	contain	containing	672:681	arg2	GLB					688:690	GLB	688:690	GLB	688:690	In method B, nano-B suspensions were obtained by adding DMF solution containing both GLB and HPMC into water.
34687818	4	57	contain	containing	672:681	arg2	HPMC					696:699	HPMC	696:699	HPMC	696:699	In method B, nano-B suspensions were obtained by adding DMF solution containing both GLB and HPMC into water.
34687818	4	57	contain	containing	672:681	arg1	solution					663:670	DMF solution	659:670	DMF solution containing both GLB and HPMC into water	659:710	In method B, nano-B suspensions were obtained by adding DMF solution containing both GLB and HPMC into water.
34687818	8	58	theme	amorphous	1246:1254	arg1	nanoparticles					1260:1272	amorphous GLB nanoparticles	1246:1272	amorphous GLB nanoparticles	1246:1272	The physical stability of amorphous GLB nanoparticles was improved with increased HPMC in the nanoparticles.
34687818	7	59	theme	amorphous	993:1001	arg1	nanoparticles					1007:1019	amorphous GLB nanoparticles	993:1019	amorphous GLB nanoparticles	993:1019	The glassy nature of the nanoparticle cores restricts the diffusion of HPMC from amorphous GLB nanoparticles to the aqueous phase, indicating that the glass transition temperature (Tg) of neat amorphous GLB (73 °C) would be considerably decreased owing to the nanosizing and water sorption of amorphous GLB.
34687818	2	60	theme	antisolvent	400:410	arg1	methods					426:432	two antisolvent precipitation methods	396:432	two antisolvent precipitation methods	396:432	Amorphous glibenclamide (GLB) nanoparticles were prepared at 4-40 °C using two antisolvent precipitation methods.
34687818	1	61	theme	amorphous	254:262	arg1	nanoparticles					269:281	amorphous drug nanoparticles	254:281	amorphous drug nanoparticles	254:281	The present study focuses on the effect of the preparation temperature on the physicochemical properties of amorphous drug nanoparticles to clarify their formation mechanism.
34687818	0	62	theme	antisolvent	62:72	arg1	method					88:93	the antisolvent precipitation method	58:93	the antisolvent precipitation method elucidated by varying the preparation temperature	58:143	Formation mechanism of amorphous drug nanoparticles using the antisolvent precipitation method elucidated by varying the preparation temperature.
34687818	1	63	from	effect	179:184	arg1	properties					240:249	the physicochemical properties	220:249	the physicochemical properties of amorphous drug nanoparticles	220:281	The present study focuses on the effect of the preparation temperature on the physicochemical properties of amorphous drug nanoparticles to clarify their formation mechanism.
34687818	3	64	theme	hydroxypropyl	536:548	arg1	HPMC					567:570	HPMC	567:570	HPMC	567:570	In method A, N,N-dimethylformamide (DMF) solution of GLB was added to an aqueous solution containing hydroxypropyl methylcellulose (HPMC) to obtain nano-A suspensions.
34687818	3	64	theme	hydroxypropyl	536:548	arg1	methylcellulose					550:564	hydroxypropyl methylcellulose	536:564	hydroxypropyl methylcellulose (HPMC) to obtain nano-A suspensions	536:600	In method A, N,N-dimethylformamide (DMF) solution of GLB was added to an aqueous solution containing hydroxypropyl methylcellulose (HPMC) to obtain nano-A suspensions.
34687818	1	65	theme	drug	264:267	arg1	nanoparticles					269:281	amorphous drug nanoparticles	254:281	amorphous drug nanoparticles	254:281	The present study focuses on the effect of the preparation temperature on the physicochemical properties of amorphous drug nanoparticles to clarify their formation mechanism.
34370460	6	0	theme	above	1270:1274	arg1	merits					1276:1281	the above merits	1266:1281	the above merits	1266:1281	Benefiting from the above merits, the proof-of-concept smart garment is assembled by the as-prepared film and is capable of regulating humidity and temperature.
34370460	2	1	theme	filtration	600:609	arg1	approach					611:618	a vacuum-assisted filtration approach	582:618	a vacuum-assisted filtration approach	582:618	In this work, a cellulose nanofiber (CNF)-based film actuator with high mechanical properties, excellent Joule heating, and antibacterial capability is successfully constructed by integrating with Ti3C2T (MXene) and tannic acid (TA) via a vacuum-assisted filtration approach.
34370460	5	2	theme	Joule	1098:1102	arg1	heating					1104:1110	outstanding Joule heating	1086:1110	outstanding Joule heating	1086:1110	With the assistance of MXene nanosheets and TA, the composite film could not only present outstanding Joule heating but also possess remarkable antibacterial properties against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus.
34370460	6	3	theme	as-prepared	1339:1349	arg1	film					1351:1354	the as-prepared film	1335:1354	the as-prepared film	1335:1354	Benefiting from the above merits, the proof-of-concept smart garment is assembled by the as-prepared film and is capable of regulating humidity and temperature.
34370460	2	4	with	actuator	398:405	arg1	capability					483:492	antibacterial capability	469:492	antibacterial capability	469:492	In this work, a cellulose nanofiber (CNF)-based film actuator with high mechanical properties, excellent Joule heating, and antibacterial capability is successfully constructed by integrating with Ti3C2T (MXene) and tannic acid (TA) via a vacuum-assisted filtration approach.
34370460	2	4	with	actuator	398:405	arg1	heating					456:462	excellent Joule heating	440:462	excellent Joule heating	440:462	In this work, a cellulose nanofiber (CNF)-based film actuator with high mechanical properties, excellent Joule heating, and antibacterial capability is successfully constructed by integrating with Ti3C2T (MXene) and tannic acid (TA) via a vacuum-assisted filtration approach.
34370460	2	4	with	actuator	398:405	arg1	properties					428:437	high mechanical properties	412:437	high mechanical properties	412:437	In this work, a cellulose nanofiber (CNF)-based film actuator with high mechanical properties, excellent Joule heating, and antibacterial capability is successfully constructed by integrating with Ti3C2T (MXene) and tannic acid (TA) via a vacuum-assisted filtration approach.
34370460	2	5	theme	vacuum-assisted	584:598	arg1	approach					611:618	a vacuum-assisted filtration approach	582:618	a vacuum-assisted filtration approach	582:618	In this work, a cellulose nanofiber (CNF)-based film actuator with high mechanical properties, excellent Joule heating, and antibacterial capability is successfully constructed by integrating with Ti3C2T (MXene) and tannic acid (TA) via a vacuum-assisted filtration approach.
34370460	1	6	theme	smart	178:182	arg1	wearables					184:192	smart wearables	178:192	smart wearables	178:192	Although humidity-responsive actuators serve as a promising candidate in smart wearables, artificial muscles, and biomimetic devices, most of them derived from synthetic polymers could not simultaneously achieve multifunctional properties.
34370460	4	7	theme	interlayer	856:865	arg1	spacing					867:873	alterable interlayer spacing	846:873	alterable interlayer spacing of MXene nanosheets	846:893	Importantly, the hydrophilic nature of CNFs and alterable interlayer spacing of MXene nanosheets endow the composite film with sensitive humidity response and extraordinary stability (1000 cycles).
34370460	6	8	theme	smart	1305:1309	arg1	garment					1311:1317	the proof-of-concept smart garment	1284:1317	the proof-of-concept smart garment	1284:1317	Benefiting from the above merits, the proof-of-concept smart garment is assembled by the as-prepared film and is capable of regulating humidity and temperature.
34370460	6	8	theme	smart	1305:1309	arg1	capable					1363:1369	capable	1363:1369	capable	1363:1369	Benefiting from the above merits, the proof-of-concept smart garment is assembled by the as-prepared film and is capable of regulating humidity and temperature.
34370460	5	9	theme	MXene	1019:1023	arg1	nanosheets					1025:1034	MXene nanosheets	1019:1034	MXene nanosheets	1019:1034	With the assistance of MXene nanosheets and TA, the composite film could not only present outstanding Joule heating but also possess remarkable antibacterial properties against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus.
34370460	1	10	theme	synthetic	265:273	arg1	polymers					275:282	synthetic polymers	265:282	synthetic polymers	265:282	Although humidity-responsive actuators serve as a promising candidate in smart wearables, artificial muscles, and biomimetic devices, most of them derived from synthetic polymers could not simultaneously achieve multifunctional properties.
34370460	4	11	theme	humidity	935:942	arg1	response					944:951	sensitive humidity response	925:951	sensitive humidity response	925:951	Importantly, the hydrophilic nature of CNFs and alterable interlayer spacing of MXene nanosheets endow the composite film with sensitive humidity response and extraordinary stability (1000 cycles).
34370460	6	12	theme	proof-of-concept	1288:1303	arg1	garment					1311:1317	the proof-of-concept smart garment	1284:1317	the proof-of-concept smart garment	1284:1317	Benefiting from the above merits, the proof-of-concept smart garment is assembled by the as-prepared film and is capable of regulating humidity and temperature.
34370460	6	12	theme	proof-of-concept	1288:1303	arg1	capable					1363:1369	capable	1363:1369	capable	1363:1369	Benefiting from the above merits, the proof-of-concept smart garment is assembled by the as-prepared film and is capable of regulating humidity and temperature.
34370460	4	13	theme	extraordinary	957:969	arg1	stability					971:979	extraordinary stability	957:979	extraordinary stability (1000 cycles)	957:993	Importantly, the hydrophilic nature of CNFs and alterable interlayer spacing of MXene nanosheets endow the composite film with sensitive humidity response and extraordinary stability (1000 cycles).
34370460	4	13	theme	extraordinary	957:969	arg1	cycles					987:992	1000 cycles	982:992	1000 cycles	982:992	Importantly, the hydrophilic nature of CNFs and alterable interlayer spacing of MXene nanosheets endow the composite film with sensitive humidity response and extraordinary stability (1000 cycles).
34370460	1	14	from	candidate	165:173	arg1	wearables					184:192	smart wearables	178:192	smart wearables	178:192	Although humidity-responsive actuators serve as a promising candidate in smart wearables, artificial muscles, and biomimetic devices, most of them derived from synthetic polymers could not simultaneously achieve multifunctional properties.
34370460	1	14	from	candidate	165:173	arg1	muscles					206:212	artificial muscles	195:212	artificial muscles	195:212	Although humidity-responsive actuators serve as a promising candidate in smart wearables, artificial muscles, and biomimetic devices, most of them derived from synthetic polymers could not simultaneously achieve multifunctional properties.
34370460	1	14	from	candidate	165:173	arg1	devices					230:236	biomimetic devices	219:236	biomimetic devices	219:236	Although humidity-responsive actuators serve as a promising candidate in smart wearables, artificial muscles, and biomimetic devices, most of them derived from synthetic polymers could not simultaneously achieve multifunctional properties.
34370460	1	15	theme	artificial	195:204	arg1	muscles					206:212	artificial muscles	195:212	artificial muscles	195:212	Although humidity-responsive actuators serve as a promising candidate in smart wearables, artificial muscles, and biomimetic devices, most of them derived from synthetic polymers could not simultaneously achieve multifunctional properties.
34370460	5	16	theme	outstanding	1086:1096	arg1	heating					1104:1110	outstanding Joule heating	1086:1110	outstanding Joule heating	1086:1110	With the assistance of MXene nanosheets and TA, the composite film could not only present outstanding Joule heating but also possess remarkable antibacterial properties against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus.
34370460	4	17	with	film	915:918	arg1	response					944:951	sensitive humidity response	925:951	sensitive humidity response	925:951	Importantly, the hydrophilic nature of CNFs and alterable interlayer spacing of MXene nanosheets endow the composite film with sensitive humidity response and extraordinary stability (1000 cycles).
34370460	4	17	with	film	915:918	arg1	stability					971:979	extraordinary stability	957:979	extraordinary stability (1000 cycles)	957:993	Importantly, the hydrophilic nature of CNFs and alterable interlayer spacing of MXene nanosheets endow the composite film with sensitive humidity response and extraordinary stability (1000 cycles).
34370460	4	17	with	film	915:918	arg1	cycles					987:992	1000 cycles	982:992	1000 cycles	982:992	Importantly, the hydrophilic nature of CNFs and alterable interlayer spacing of MXene nanosheets endow the composite film with sensitive humidity response and extraordinary stability (1000 cycles).
34370460	2	18	theme	film	393:396	arg1	actuator					398:405	a cellulose nanofiber (CNF)-based film actuator	359:405	a cellulose nanofiber (CNF)-based film actuator with high mechanical properties, excellent Joule heating, and antibacterial capability	359:492	In this work, a cellulose nanofiber (CNF)-based film actuator with high mechanical properties, excellent Joule heating, and antibacterial capability is successfully constructed by integrating with Ti3C2T (MXene) and tannic acid (TA) via a vacuum-assisted filtration approach.
34370460	3	19	dep	strength	700:707	arg1	the					688:690	the	688:690	the	688:690	Owing to the unique nacrelike structure and strong hydrogen bonds, the tensile strength and toughness of the composite film could reach 275.4 MPa and 10.2 MJ·m-3, respectively.
34370460	2	20	theme	-based	386:391	arg1	actuator					398:405	a cellulose nanofiber (CNF)-based film actuator	359:405	a cellulose nanofiber (CNF)-based film actuator with high mechanical properties, excellent Joule heating, and antibacterial capability	359:492	In this work, a cellulose nanofiber (CNF)-based film actuator with high mechanical properties, excellent Joule heating, and antibacterial capability is successfully constructed by integrating with Ti3C2T (MXene) and tannic acid (TA) via a vacuum-assisted filtration approach.
34370460	2	21	theme	Joule	450:454	arg1	heating					456:462	excellent Joule heating	440:462	excellent Joule heating	440:462	In this work, a cellulose nanofiber (CNF)-based film actuator with high mechanical properties, excellent Joule heating, and antibacterial capability is successfully constructed by integrating with Ti3C2T (MXene) and tannic acid (TA) via a vacuum-assisted filtration approach.
34370460	4	22	theme	CNFs	837:840	arg1	nature					827:832	the hydrophilic nature	811:832	the hydrophilic nature of CNFs	811:840	Importantly, the hydrophilic nature of CNFs and alterable interlayer spacing of MXene nanosheets endow the composite film with sensitive humidity response and extraordinary stability (1000 cycles).
34370460	4	22	theme	CNFs	837:840	arg1	spacing					867:873	alterable interlayer spacing	846:873	alterable interlayer spacing of MXene nanosheets	846:893	Importantly, the hydrophilic nature of CNFs and alterable interlayer spacing of MXene nanosheets endow the composite film with sensitive humidity response and extraordinary stability (1000 cycles).
34370460	4	23	theme	alterable	846:854	arg1	spacing					867:873	alterable interlayer spacing	846:873	alterable interlayer spacing of MXene nanosheets	846:893	Importantly, the hydrophilic nature of CNFs and alterable interlayer spacing of MXene nanosheets endow the composite film with sensitive humidity response and extraordinary stability (1000 cycles).
34370460	2	24	theme	excellent	440:448	arg1	heating					456:462	excellent Joule heating	440:462	excellent Joule heating	440:462	In this work, a cellulose nanofiber (CNF)-based film actuator with high mechanical properties, excellent Joule heating, and antibacterial capability is successfully constructed by integrating with Ti3C2T (MXene) and tannic acid (TA) via a vacuum-assisted filtration approach.
34370460	2	25	theme	tannic	561:566	arg1	TA					574:575	TA	574:575	TA	574:575	In this work, a cellulose nanofiber (CNF)-based film actuator with high mechanical properties, excellent Joule heating, and antibacterial capability is successfully constructed by integrating with Ti3C2T (MXene) and tannic acid (TA) via a vacuum-assisted filtration approach.
34370460	2	25	theme	tannic	561:566	arg1	acid					568:571	tannic acid	561:571	tannic acid (TA)	561:576	In this work, a cellulose nanofiber (CNF)-based film actuator with high mechanical properties, excellent Joule heating, and antibacterial capability is successfully constructed by integrating with Ti3C2T (MXene) and tannic acid (TA) via a vacuum-assisted filtration approach.
34370460	0	26	theme	Multifunctional	10:24	arg1	Actuators					41:49	Multifunctional Composite Film Actuators	10:49	Multifunctional Composite Film Actuators	10:49	Tough and Multifunctional Composite Film Actuators Based on Cellulose Nanofibers toward Smart Wearables.
34370460	3	27	theme	composite	730:738	arg1	film					740:743	the composite film	726:743	the composite film	726:743	Owing to the unique nacrelike structure and strong hydrogen bonds, the tensile strength and toughness of the composite film could reach 275.4 MPa and 10.2 MJ·m-3, respectively.
34370460	4	28	theme	composite	905:913	arg1	film					915:918	the composite film	901:918	the composite film with sensitive humidity response and extraordinary stability (1000 cycles)	901:993	Importantly, the hydrophilic nature of CNFs and alterable interlayer spacing of MXene nanosheets endow the composite film with sensitive humidity response and extraordinary stability (1000 cycles).
34370460	3	29	theme	nacrelike	641:649	arg1	structure					651:659	the unique nacrelike structure	630:659	the unique nacrelike structure	630:659	Owing to the unique nacrelike structure and strong hydrogen bonds, the tensile strength and toughness of the composite film could reach 275.4 MPa and 10.2 MJ·m-3, respectively.
34370460	1	30	theme	humidity-responsive	114:132	arg1	candidate					165:173	a promising candidate	153:173	a promising candidate in smart wearables, artificial muscles, and biomimetic devices	153:236	Although humidity-responsive actuators serve as a promising candidate in smart wearables, artificial muscles, and biomimetic devices, most of them derived from synthetic polymers could not simultaneously achieve multifunctional properties.
34370460	1	30	theme	humidity-responsive	114:132	arg1	actuators					134:142	humidity-responsive actuators	114:142	humidity-responsive actuators	114:142	Although humidity-responsive actuators serve as a promising candidate in smart wearables, artificial muscles, and biomimetic devices, most of them derived from synthetic polymers could not simultaneously achieve multifunctional properties.
34370460	2	31	theme	mechanical	417:426	arg1	properties					428:437	high mechanical properties	412:437	high mechanical properties	412:437	In this work, a cellulose nanofiber (CNF)-based film actuator with high mechanical properties, excellent Joule heating, and antibacterial capability is successfully constructed by integrating with Ti3C2T (MXene) and tannic acid (TA) via a vacuum-assisted filtration approach.
34370460	3	32	theme	film	740:743	arg1	toughness					713:721	toughness	713:721	toughness	713:721	Owing to the unique nacrelike structure and strong hydrogen bonds, the tensile strength and toughness of the composite film could reach 275.4 MPa and 10.2 MJ·m-3, respectively.
34370460	3	32	theme	film	740:743	arg1	strength					700:707	tensile strength	692:707	tensile strength	692:707	Owing to the unique nacrelike structure and strong hydrogen bonds, the tensile strength and toughness of the composite film could reach 275.4 MPa and 10.2 MJ·m-3, respectively.
34370460	4	33	theme	sensitive	925:933	arg1	response					944:951	sensitive humidity response	925:951	sensitive humidity response	925:951	Importantly, the hydrophilic nature of CNFs and alterable interlayer spacing of MXene nanosheets endow the composite film with sensitive humidity response and extraordinary stability (1000 cycles).
34370460	1	34	theme	biomimetic	219:228	arg1	devices					230:236	biomimetic devices	219:236	biomimetic devices	219:236	Although humidity-responsive actuators serve as a promising candidate in smart wearables, artificial muscles, and biomimetic devices, most of them derived from synthetic polymers could not simultaneously achieve multifunctional properties.
34370460	2	35	theme	high	412:415	arg1	properties					428:437	high mechanical properties	412:437	high mechanical properties	412:437	In this work, a cellulose nanofiber (CNF)-based film actuator with high mechanical properties, excellent Joule heating, and antibacterial capability is successfully constructed by integrating with Ti3C2T (MXene) and tannic acid (TA) via a vacuum-assisted filtration approach.
34370460	2	36	theme	antibacterial	469:481	arg1	capability					483:492	antibacterial capability	469:492	antibacterial capability	469:492	In this work, a cellulose nanofiber (CNF)-based film actuator with high mechanical properties, excellent Joule heating, and antibacterial capability is successfully constructed by integrating with Ti3C2T (MXene) and tannic acid (TA) via a vacuum-assisted filtration approach.
34370460	1	37	theme	multifunctional	317:331	arg1	properties					333:342	multifunctional properties	317:342	multifunctional properties	317:342	Although humidity-responsive actuators serve as a promising candidate in smart wearables, artificial muscles, and biomimetic devices, most of them derived from synthetic polymers could not simultaneously achieve multifunctional properties.
34370460	0	38	theme	Film	36:39	arg1	Actuators					41:49	Multifunctional Composite Film Actuators	10:49	Multifunctional Composite Film Actuators	10:49	Tough and Multifunctional Composite Film Actuators Based on Cellulose Nanofibers toward Smart Wearables.
34370460	0	39	theme	Composite	26:34	arg1	Actuators					41:49	Multifunctional Composite Film Actuators	10:49	Multifunctional Composite Film Actuators	10:49	Tough and Multifunctional Composite Film Actuators Based on Cellulose Nanofibers toward Smart Wearables.
34370460	5	40	theme	composite	1048:1056	arg1	film					1058:1061	the composite film	1044:1061	the composite film	1044:1061	With the assistance of MXene nanosheets and TA, the composite film could not only present outstanding Joule heating but also possess remarkable antibacterial properties against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus.
34370460	5	41	theme	remarkable	1129:1138	arg1	properties					1154:1163	remarkable antibacterial properties	1129:1163	remarkable antibacterial properties	1129:1163	With the assistance of MXene nanosheets and TA, the composite film could not only present outstanding Joule heating but also possess remarkable antibacterial properties against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus.
34370460	4	42	theme	nanosheets	884:893	arg1	nature					827:832	the hydrophilic nature	811:832	the hydrophilic nature of CNFs	811:840	Importantly, the hydrophilic nature of CNFs and alterable interlayer spacing of MXene nanosheets endow the composite film with sensitive humidity response and extraordinary stability (1000 cycles).
34370460	4	42	theme	nanosheets	884:893	arg1	spacing					867:873	alterable interlayer spacing	846:873	alterable interlayer spacing of MXene nanosheets	846:893	Importantly, the hydrophilic nature of CNFs and alterable interlayer spacing of MXene nanosheets endow the composite film with sensitive humidity response and extraordinary stability (1000 cycles).
34370460	3	43	theme	unique	634:639	arg1	structure					651:659	the unique nacrelike structure	630:659	the unique nacrelike structure	630:659	Owing to the unique nacrelike structure and strong hydrogen bonds, the tensile strength and toughness of the composite film could reach 275.4 MPa and 10.2 MJ·m-3, respectively.
34370460	4	44	theme	hydrophilic	815:825	arg1	nature					827:832	the hydrophilic nature	811:832	the hydrophilic nature of CNFs	811:840	Importantly, the hydrophilic nature of CNFs and alterable interlayer spacing of MXene nanosheets endow the composite film with sensitive humidity response and extraordinary stability (1000 cycles).
34370460	4	45	theme	MXene	878:882	arg1	nanosheets					884:893	MXene nanosheets	878:893	MXene nanosheets	878:893	Importantly, the hydrophilic nature of CNFs and alterable interlayer spacing of MXene nanosheets endow the composite film with sensitive humidity response and extraordinary stability (1000 cycles).
34370460	3	46	theme	hydrogen	672:679	arg1	bonds					681:685	strong hydrogen bonds	665:685	strong hydrogen bonds	665:685	Owing to the unique nacrelike structure and strong hydrogen bonds, the tensile strength and toughness of the composite film could reach 275.4 MPa and 10.2 MJ·m-3, respectively.
34370460	5	47	theme	nanosheets	1025:1034	arg1	assistance					1005:1014	the assistance	1001:1014	the assistance of MXene nanosheets and TA	1001:1041	With the assistance of MXene nanosheets and TA, the composite film could not only present outstanding Joule heating but also possess remarkable antibacterial properties against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus.
34370460	5	48	theme	TA	1040:1041	arg1	assistance					1005:1014	the assistance	1001:1014	the assistance of MXene nanosheets and TA	1001:1041	With the assistance of MXene nanosheets and TA, the composite film could not only present outstanding Joule heating but also possess remarkable antibacterial properties against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus.
34370460	1	49	attach	derived	252:258	arg2	them					247:250	them	247:250	them	247:250	Although humidity-responsive actuators serve as a promising candidate in smart wearables, artificial muscles, and biomimetic devices, most of them derived from synthetic polymers could not simultaneously achieve multifunctional properties.
34370460	1	49	attach	derived	252:258	arg2	most					239:242	most	239:242	most	239:242	Although humidity-responsive actuators serve as a promising candidate in smart wearables, artificial muscles, and biomimetic devices, most of them derived from synthetic polymers could not simultaneously achieve multifunctional properties.
34370460	1	49	attach	derived	252:258	arg1	polymers					275:282	synthetic polymers	265:282	synthetic polymers	265:282	Although humidity-responsive actuators serve as a promising candidate in smart wearables, artificial muscles, and biomimetic devices, most of them derived from synthetic polymers could not simultaneously achieve multifunctional properties.
34370460	5	50	contain	possess	1121:1127	arg1	film					1058:1061	the composite film	1044:1061	the composite film	1044:1061	With the assistance of MXene nanosheets and TA, the composite film could not only present outstanding Joule heating but also possess remarkable antibacterial properties against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus.
34370460	5	50	contain	possess	1121:1127	arg2	properties					1154:1163	remarkable antibacterial properties	1129:1163	remarkable antibacterial properties	1129:1163	With the assistance of MXene nanosheets and TA, the composite film could not only present outstanding Joule heating but also possess remarkable antibacterial properties against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus.
34370460	3	51	theme	strong	665:670	arg1	bonds					681:685	strong hydrogen bonds	665:685	strong hydrogen bonds	665:685	Owing to the unique nacrelike structure and strong hydrogen bonds, the tensile strength and toughness of the composite film could reach 275.4 MPa and 10.2 MJ·m-3, respectively.
34370460	1	52	theme	promising	155:163	arg1	candidate					165:173	a promising candidate	153:173	a promising candidate in smart wearables, artificial muscles, and biomimetic devices	153:236	Although humidity-responsive actuators serve as a promising candidate in smart wearables, artificial muscles, and biomimetic devices, most of them derived from synthetic polymers could not simultaneously achieve multifunctional properties.
34370460	1	52	theme	promising	155:163	arg1	actuators					134:142	humidity-responsive actuators	114:142	humidity-responsive actuators	114:142	Although humidity-responsive actuators serve as a promising candidate in smart wearables, artificial muscles, and biomimetic devices, most of them derived from synthetic polymers could not simultaneously achieve multifunctional properties.
34370460	0	53	theme	Cellulose	60:68	arg1	Nanofibers					70:79	Cellulose Nanofibers	60:79	Cellulose Nanofibers toward Smart Wearables	60:102	Tough and Multifunctional Composite Film Actuators Based on Cellulose Nanofibers toward Smart Wearables.
34370460	3	54	theme	tensile	692:698	arg1	strength					700:707	tensile strength	692:707	tensile strength	692:707	Owing to the unique nacrelike structure and strong hydrogen bonds, the tensile strength and toughness of the composite film could reach 275.4 MPa and 10.2 MJ·m-3, respectively.
34370460	5	55	theme	antibacterial	1140:1152	arg1	properties					1154:1163	remarkable antibacterial properties	1129:1163	remarkable antibacterial properties	1129:1163	With the assistance of MXene nanosheets and TA, the composite film could not only present outstanding Joule heating but also possess remarkable antibacterial properties against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus.
33812606	2	0	theme	post-operative	850:863	arg1	adhesion					865:872	post-operative adhesion	850:872	post-operative adhesion	850:872	The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion.
33812606	1	1	theme	hydrogels	257:265	arg1	materials					289:297	novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials	188:297	novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA)	188:373	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	0	2	theme	wound	91:95	arg1	Antibacterial					0:12	Antibacterial	0:12	Antibacterial	0:12	Antibacterial, hemostasis, adhesive, self-healing polysaccharides-based composite hydrogel wound dressing for the prevention and treatment of postoperative adhesion.
33812606	0	2	theme	wound	91:95	arg1	dressing					97:104	self-healing polysaccharides-based composite hydrogel wound dressing	37:104	self-healing polysaccharides-based composite hydrogel wound dressing for the prevention and treatment of postoperative adhesion	37:163	Antibacterial, hemostasis, adhesive, self-healing polysaccharides-based composite hydrogel wound dressing for the prevention and treatment of postoperative adhesion.
33812606	1	3	theme	external	429:436	arg1	stimuli					438:444	external stimuli	429:444	external stimuli	429:444	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	3	4	theme	rat	888:890	arg1	model					923:927	a rat injury sidewall-cecum abrasion model	886:927	a rat injury sidewall-cecum abrasion model	886:927	Meanwhile, a rat injury sidewall-cecum abrasion model is developed to investigate the efficacy of these hydrogels in achieving post-operative anti-adhesion.
33812606	3	4	theme	rat	888:890	arg1	Meanwhile					875:883	Meanwhile	875:883	Meanwhile	875:883	Meanwhile, a rat injury sidewall-cecum abrasion model is developed to investigate the efficacy of these hydrogels in achieving post-operative anti-adhesion.
33812606	1	5	theme	anti-adhesion	267:279	arg1	materials					289:297	novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials	188:297	novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA)	188:373	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	2	6	theme	good	589:592	arg1	cytocompatibility					594:610	good cytocompatibility	589:610	good cytocompatibility	589:610	The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion.
33812606	2	7	theme	excellent	664:672	arg1	biodegradable					674:686	excellent biodegradable	664:686	excellent biodegradable	664:686	The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion.
33812606	1	8	dep	prevention	463:472	arg1	the					459:461	the	459:461	the	459:461	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	1	9	theme	barrier	281:287	arg1	materials					289:297	novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials	188:297	novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA)	188:373	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	1	10	theme	oxidized	352:359	arg1	dextran					361:367	oxidized dextran	352:367	oxidized dextran (ODA)	352:373	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	1	10	theme	oxidized	352:359	arg1	ODA					370:372	ODA	370:372	ODA	370:372	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	4	11	located	observed	1119:1126	arg1	N					1135:1135	the N	1131:1135	the N	1131:1135	A significant reduction of peritoneal adhesions (10% rat with lower score adhesion) is observed in the N,O-CS/ODA-hydrogel-treated group compared with the commercial hydrogel and control groups.
33812606	4	11	located	observed	1119:1126	arg1	group					1163:1167	O-CS/ODA-hydrogel-treated group	1137:1167	O-CS/ODA-hydrogel-treated group compared with the commercial hydrogel and control groups	1137:1224	A significant reduction of peritoneal adhesions (10% rat with lower score adhesion) is observed in the N,O-CS/ODA-hydrogel-treated group compared with the commercial hydrogel and control groups.
33812606	4	11	located	observed	1119:1126	arg2	reduction					1046:1054	A significant reduction	1032:1054	A significant reduction of peritoneal adhesions (10% rat with lower score adhesion)	1032:1114	A significant reduction of peritoneal adhesions (10% rat with lower score adhesion) is observed in the N,O-CS/ODA-hydrogel-treated group compared with the commercial hydrogel and control groups.
33812606	0	12	dep	prevention	114:123	arg1	the					110:112	the	110:112	the	110:112	Antibacterial, hemostasis, adhesive, self-healing polysaccharides-based composite hydrogel wound dressing for the prevention and treatment of postoperative adhesion.
33812606	6	13	dep	prevention	1463:1472	arg1	the					1459:1461	the	1459:1461	the	1459:1461	Therefore, the N,O-CS/ODA hydrogels with multi-functional properties exhibit great potential for the prevention and treatment of postoperative adhesion.
33812606	6	14	theme	multi-functional	1403:1418	arg1	properties					1420:1429	multi-functional properties	1403:1429	multi-functional properties	1403:1429	Therefore, the N,O-CS/ODA hydrogels with multi-functional properties exhibit great potential for the prevention and treatment of postoperative adhesion.
33812606	1	15	dep	N	340:340	arg1	O-CS					342:345	O-CS	342:345	O-CS	342:345	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	2	16	theme	antibacterial	640:652	arg1	activity					654:661	good antibacterial activity	635:661	good antibacterial activity	635:661	The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion.
33812606	3	17	theme	abrasion	914:921	arg1	model					923:927	a rat injury sidewall-cecum abrasion model	886:927	a rat injury sidewall-cecum abrasion model	886:927	Meanwhile, a rat injury sidewall-cecum abrasion model is developed to investigate the efficacy of these hydrogels in achieving post-operative anti-adhesion.
33812606	3	17	theme	abrasion	914:921	arg1	Meanwhile					875:883	Meanwhile	875:883	Meanwhile	875:883	Meanwhile, a rat injury sidewall-cecum abrasion model is developed to investigate the efficacy of these hydrogels in achieving post-operative anti-adhesion.
33812606	4	18	theme	lower	1094:1098	arg1	adhesion					1106:1113	lower score adhesion	1094:1113	lower score adhesion	1094:1113	A significant reduction of peritoneal adhesions (10% rat with lower score adhesion) is observed in the N,O-CS/ODA-hydrogel-treated group compared with the commercial hydrogel and control groups.
33812606	4	19	theme	O-CS/ODA-hydrogel-treated	1137:1161	arg1	N					1135:1135	the N	1131:1135	the N	1131:1135	A significant reduction of peritoneal adhesions (10% rat with lower score adhesion) is observed in the N,O-CS/ODA-hydrogel-treated group compared with the commercial hydrogel and control groups.
33812606	4	19	theme	O-CS/ODA-hydrogel-treated	1137:1161	arg1	group					1163:1167	O-CS/ODA-hydrogel-treated group	1137:1167	O-CS/ODA-hydrogel-treated group compared with the commercial hydrogel and control groups	1137:1224	A significant reduction of peritoneal adhesions (10% rat with lower score adhesion) is observed in the N,O-CS/ODA-hydrogel-treated group compared with the commercial hydrogel and control groups.
33812606	1	20	dep	agent	420:424	arg1	triggers					446:453	triggers	446:453	triggers for the prevention and treatment of post-operative peritoneal adhesions	446:525	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	4	21	theme	control	1211:1217	arg1	groups					1219:1224	the commercial hydrogel and control groups	1183:1224	groups	1219:1224	A significant reduction of peritoneal adhesions (10% rat with lower score adhesion) is observed in the N,O-CS/ODA-hydrogel-treated group compared with the commercial hydrogel and control groups.
33812606	2	22	contain	have	553:556	arg2	biocompatible					692:704	biocompatible	692:704	biocompatible	692:704	The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion.
33812606	2	22	contain	have	553:556	arg1	N					532:532	The N	528:532	The N	528:532	The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion.
33812606	2	22	contain	have	553:556	arg1	hydrogels					543:551	O-CS/ODA hydrogels	534:551	O-CS/ODA hydrogels	534:551	The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion.
33812606	2	22	contain	have	553:556	arg2	cytocompatibility					594:610	good cytocompatibility	589:610	good cytocompatibility	589:610	The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion.
33812606	2	22	contain	have	553:556	arg2	time					583:586	a good suitable gelation time	558:586	a good suitable gelation time	558:586	The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion.
33812606	2	22	contain	have	553:556	arg2	activity					654:661	good antibacterial activity	635:661	good antibacterial activity	635:661	The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion.
33812606	2	22	contain	have	553:556	arg2	biodegradable					674:686	excellent biodegradable	664:686	excellent biodegradable	664:686	The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion.
33812606	1	23	theme	novel	188:192	arg1	materials					289:297	novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials	188:297	novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA)	188:373	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	5	24	theme	post-operative	1305:1318	arg1	adhesion					1331:1338	post-operative peritoneal adhesion	1305:1338	post-operative peritoneal adhesion	1305:1338	These results demonstrated that N,O-CS/ODA hydrogel could effectively prevent post-operative peritoneal adhesion without side effects.
33812606	6	25	theme	great	1439:1443	arg1	potential					1445:1453	great potential	1439:1453	great potential for the prevention and treatment of postoperative adhesion	1439:1512	Therefore, the N,O-CS/ODA hydrogels with multi-functional properties exhibit great potential for the prevention and treatment of postoperative adhesion.
33812606	4	26	dep	adhesions	1070:1078	arg1	rat					1085:1087	10% rat	1081:1087	10% rat with lower score adhesion	1081:1113	A significant reduction of peritoneal adhesions (10% rat with lower score adhesion) is observed in the N,O-CS/ODA-hydrogel-treated group compared with the commercial hydrogel and control groups.
33812606	1	27	theme	self-healing	194:205	arg1	materials					289:297	novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials	188:297	novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA)	188:373	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	4	28	theme	adhesions	1070:1078	arg1	reduction					1046:1054	A significant reduction	1032:1054	A significant reduction of peritoneal adhesions (10% rat with lower score adhesion)	1032:1114	A significant reduction of peritoneal adhesions (10% rat with lower score adhesion) is observed in the N,O-CS/ODA-hydrogel-treated group compared with the commercial hydrogel and control groups.
33812606	4	29	with	rat	1085:1087	arg1	adhesion					1106:1113	lower score adhesion	1094:1113	lower score adhesion	1094:1113	A significant reduction of peritoneal adhesions (10% rat with lower score adhesion) is observed in the N,O-CS/ODA-hydrogel-treated group compared with the commercial hydrogel and control groups.
33812606	4	30	theme	peritoneal	1059:1068	arg1	adhesions					1070:1078	peritoneal adhesions	1059:1078	peritoneal adhesions (10% rat with lower score adhesion)	1059:1114	A significant reduction of peritoneal adhesions (10% rat with lower score adhesion) is observed in the N,O-CS/ODA-hydrogel-treated group compared with the commercial hydrogel and control groups.
33812606	4	31	theme	%	1083:1083	arg1	rat					1085:1087	10% rat	1081:1087	10% rat with lower score adhesion	1081:1113	A significant reduction of peritoneal adhesions (10% rat with lower score adhesion) is observed in the N,O-CS/ODA-hydrogel-treated group compared with the commercial hydrogel and control groups.
33812606	6	32	with	hydrogels	1388:1396	arg1	properties					1420:1429	multi-functional properties	1403:1429	multi-functional properties	1403:1429	Therefore, the N,O-CS/ODA hydrogels with multi-functional properties exhibit great potential for the prevention and treatment of postoperative adhesion.
33812606	1	33	theme	in	208:209	arg1	materials					289:297	novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials	188:297	novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA)	188:373	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	4	34	theme	10	1081:1082	arg1	%					1083:1083	%	1083:1083	%	1083:1083	A significant reduction of peritoneal adhesions (10% rat with lower score adhesion) is observed in the N,O-CS/ODA-hydrogel-treated group compared with the commercial hydrogel and control groups.
33812606	6	35	theme	O-CS/ODA	1379:1386	arg1	hydrogels					1388:1396	the N,O-CS/ODA hydrogels	1373:1396	hydrogels	1388:1396	Therefore, the N,O-CS/ODA hydrogels with multi-functional properties exhibit great potential for the prevention and treatment of postoperative adhesion.
33812606	5	36	theme	peritoneal	1320:1329	arg1	adhesion					1331:1338	post-operative peritoneal adhesion	1305:1338	post-operative peritoneal adhesion	1305:1338	These results demonstrated that N,O-CS/ODA hydrogel could effectively prevent post-operative peritoneal adhesion without side effects.
33812606	0	37	theme	adhesion	156:163	arg1	treatment					129:137	treatment	129:137	treatment	129:137	Antibacterial, hemostasis, adhesive, self-healing polysaccharides-based composite hydrogel wound dressing for the prevention and treatment of postoperative adhesion.
33812606	0	37	theme	adhesion	156:163	arg1	prevention					114:123	prevention	114:123	prevention	114:123	Antibacterial, hemostasis, adhesive, self-healing polysaccharides-based composite hydrogel wound dressing for the prevention and treatment of postoperative adhesion.
33812606	4	38	theme	score	1100:1104	arg1	adhesion					1106:1113	lower score adhesion	1094:1113	lower score adhesion	1094:1113	A significant reduction of peritoneal adhesions (10% rat with lower score adhesion) is observed in the N,O-CS/ODA-hydrogel-treated group compared with the commercial hydrogel and control groups.
33812606	4	39	theme	significant	1034:1044	arg1	reduction					1046:1054	A significant reduction	1032:1054	A significant reduction of peritoneal adhesions (10% rat with lower score adhesion)	1032:1114	A significant reduction of peritoneal adhesions (10% rat with lower score adhesion) is observed in the N,O-CS/ODA-hydrogel-treated group compared with the commercial hydrogel and control groups.
33812606	1	40	theme	O-carboxymethyl	314:328	arg1	chitosan					330:337	O-carboxymethyl chitosan	314:337	O-carboxymethyl chitosan (N,O-CS)	314:346	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	1	40	theme	O-carboxymethyl	314:328	arg1	N					340:340	N	340:340	N	340:340	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	0	41	theme	postoperative	142:154	arg1	adhesion					156:163	postoperative adhesion	142:163	postoperative adhesion	142:163	Antibacterial, hemostasis, adhesive, self-healing polysaccharides-based composite hydrogel wound dressing for the prevention and treatment of postoperative adhesion.
33812606	6	42	theme	adhesion	1505:1512	arg1	treatment					1478:1486	treatment	1478:1486	treatment	1478:1486	Therefore, the N,O-CS/ODA hydrogels with multi-functional properties exhibit great potential for the prevention and treatment of postoperative adhesion.
33812606	6	42	theme	adhesion	1505:1512	arg1	prevention					1463:1472	prevention	1463:1472	prevention	1463:1472	Therefore, the N,O-CS/ODA hydrogels with multi-functional properties exhibit great potential for the prevention and treatment of postoperative adhesion.
33812606	2	43	theme	O-CS/ODA	534:541	arg1	N					532:532	The N	528:532	The N	528:532	The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion.
33812606	2	43	theme	O-CS/ODA	534:541	arg1	hydrogels					543:551	O-CS/ODA hydrogels	534:551	O-CS/ODA hydrogels	534:551	The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion.
33812606	0	44	theme	polysaccharides-based	50:70	arg1	Antibacterial					0:12	Antibacterial	0:12	Antibacterial	0:12	Antibacterial, hemostasis, adhesive, self-healing polysaccharides-based composite hydrogel wound dressing for the prevention and treatment of postoperative adhesion.
33812606	0	44	theme	polysaccharides-based	50:70	arg1	dressing					97:104	self-healing polysaccharides-based composite hydrogel wound dressing	37:104	self-healing polysaccharides-based composite hydrogel wound dressing for the prevention and treatment of postoperative adhesion	37:163	Antibacterial, hemostasis, adhesive, self-healing polysaccharides-based composite hydrogel wound dressing for the prevention and treatment of postoperative adhesion.
33812606	3	45	theme	hydrogels	979:987	arg1	efficacy					961:968	the efficacy	957:968	the efficacy of these hydrogels in achieving post-operative anti-adhesion	957:1029	Meanwhile, a rat injury sidewall-cecum abrasion model is developed to investigate the efficacy of these hydrogels in achieving post-operative anti-adhesion.
33812606	1	46	theme	post-operative	491:504	arg1	adhesions					517:525	post-operative peritoneal adhesions	491:525	post-operative peritoneal adhesions	491:525	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	0	47	theme	self-healing	37:48	arg1	Antibacterial					0:12	Antibacterial	0:12	Antibacterial	0:12	Antibacterial, hemostasis, adhesive, self-healing polysaccharides-based composite hydrogel wound dressing for the prevention and treatment of postoperative adhesion.
33812606	0	47	theme	self-healing	37:48	arg1	dressing					97:104	self-healing polysaccharides-based composite hydrogel wound dressing	37:104	self-healing polysaccharides-based composite hydrogel wound dressing for the prevention and treatment of postoperative adhesion	37:163	Antibacterial, hemostasis, adhesive, self-healing polysaccharides-based composite hydrogel wound dressing for the prevention and treatment of postoperative adhesion.
33812606	2	48	theme	gelation	574:581	arg1	time					583:586	a good suitable gelation time	558:586	a good suitable gelation time	558:586	The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion.
33812606	1	49	theme	chemical	397:404	arg1	agent					420:424	any chemical cross-linking agent	393:424	any chemical cross-linking agent	393:424	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	2	50	theme	good	635:638	arg1	activity					654:661	good antibacterial activity	635:661	good antibacterial activity	635:661	The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion.
33812606	1	51	theme	peritoneal	506:515	arg1	adhesions					517:525	post-operative peritoneal adhesions	491:525	post-operative peritoneal adhesions	491:525	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	2	52	theme	suitable	565:572	arg1	time					583:586	a good suitable gelation time	558:586	a good suitable gelation time	558:586	The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion.
33812606	1	53	theme	cross-linking	406:418	arg1	agent					420:424	any chemical cross-linking agent	393:424	any chemical cross-linking agent	393:424	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	1	54	theme	adhesions	517:525	arg1	prevention					463:472	prevention	463:472	prevention	463:472	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	1	54	theme	adhesions	517:525	arg1	treatment					478:486	treatment	478:486	treatment	478:486	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	6	55	theme	N	1377:1377	arg1	hydrogels					1388:1396	the N,O-CS/ODA hydrogels	1373:1396	hydrogels	1388:1396	Therefore, the N,O-CS/ODA hydrogels with multi-functional properties exhibit great potential for the prevention and treatment of postoperative adhesion.
33812606	1	56	theme	biodegradable	228:240	arg1	materials					289:297	novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials	188:297	novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA)	188:373	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	2	57	theme	good	560:563	arg1	time					583:586	a good suitable gelation time	558:586	a good suitable gelation time	558:586	The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion.
33812606	4	58	theme	commercial	1187:1196	arg1	hydrogel					1198:1205	the commercial hydrogel and control groups	1183:1224	hydrogel	1198:1205	A significant reduction of peritoneal adhesions (10% rat with lower score adhesion) is observed in the N,O-CS/ODA-hydrogel-treated group compared with the commercial hydrogel and control groups.
33812606	5	59	theme	side	1348:1351	arg1	effects					1353:1359	side effects	1348:1359	side effects	1348:1359	These results demonstrated that N,O-CS/ODA hydrogel could effectively prevent post-operative peritoneal adhesion without side effects.
33812606	5	60	theme	O-CS/ODA	1261:1268	arg1	N					1259:1259	N	1259:1259	N	1259:1259	These results demonstrated that N,O-CS/ODA hydrogel could effectively prevent post-operative peritoneal adhesion without side effects.
33812606	5	60	theme	O-CS/ODA	1261:1268	arg1	hydrogel					1270:1277	O-CS/ODA hydrogel	1261:1277	O-CS/ODA hydrogel	1261:1277	These results demonstrated that N,O-CS/ODA hydrogel could effectively prevent post-operative peritoneal adhesion without side effects.
33812606	0	61	theme	hydrogel	82:89	arg1	Antibacterial					0:12	Antibacterial	0:12	Antibacterial	0:12	Antibacterial, hemostasis, adhesive, self-healing polysaccharides-based composite hydrogel wound dressing for the prevention and treatment of postoperative adhesion.
33812606	0	61	theme	hydrogel	82:89	arg1	dressing					97:104	self-healing polysaccharides-based composite hydrogel wound dressing	37:104	self-healing polysaccharides-based composite hydrogel wound dressing for the prevention and treatment of postoperative adhesion	37:163	Antibacterial, hemostasis, adhesive, self-healing polysaccharides-based composite hydrogel wound dressing for the prevention and treatment of postoperative adhesion.
33812606	3	62	theme	injury	892:897	arg1	model					923:927	a rat injury sidewall-cecum abrasion model	886:927	a rat injury sidewall-cecum abrasion model	886:927	Meanwhile, a rat injury sidewall-cecum abrasion model is developed to investigate the efficacy of these hydrogels in achieving post-operative anti-adhesion.
33812606	3	62	theme	injury	892:897	arg1	Meanwhile					875:883	Meanwhile	875:883	Meanwhile	875:883	Meanwhile, a rat injury sidewall-cecum abrasion model is developed to investigate the efficacy of these hydrogels in achieving post-operative anti-adhesion.
33812606	1	63	dep	in	208:209	arg1	situ					211:214	situ	211:214	situ	211:214	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	1	63	dep	in	208:209	arg1	injectable					216:225	injectable	216:225	injectable	216:225	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
33812606	2	64	theme	fibroblasts	751:761	arg1	adhesion					739:746	the adhesion	735:746	the adhesion of fibroblasts to the wound	735:774	The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion.
33812606	2	65	theme	O-CS/ODA	803:810	arg1	N					801:801	N	801:801	N	801:801	The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion.
33812606	2	65	theme	O-CS/ODA	803:810	arg1	hydrogels					812:820	O-CS/ODA hydrogels	803:820	O-CS/ODA hydrogels	803:820	The N,O-CS/ODA hydrogels have a good suitable gelation time, good cytocompatibility and hemocompatibility, good antibacterial activity, excellent biodegradable and biocompatible, and can effectively inhibit the adhesion of fibroblasts to the wound, thereby suggesting that N,O-CS/ODA hydrogels are suitable for preventing post-operative adhesion.
33812606	0	66	theme	composite	72:80	arg1	Antibacterial					0:12	Antibacterial	0:12	Antibacterial	0:12	Antibacterial, hemostasis, adhesive, self-healing polysaccharides-based composite hydrogel wound dressing for the prevention and treatment of postoperative adhesion.
33812606	0	66	theme	composite	72:80	arg1	dressing					97:104	self-healing polysaccharides-based composite hydrogel wound dressing	37:104	self-healing polysaccharides-based composite hydrogel wound dressing for the prevention and treatment of postoperative adhesion	37:163	Antibacterial, hemostasis, adhesive, self-healing polysaccharides-based composite hydrogel wound dressing for the prevention and treatment of postoperative adhesion.
33812606	6	67	theme	postoperative	1491:1503	arg1	adhesion					1505:1512	postoperative adhesion	1491:1512	postoperative adhesion	1491:1512	Therefore, the N,O-CS/ODA hydrogels with multi-functional properties exhibit great potential for the prevention and treatment of postoperative adhesion.
33812606	3	68	theme	post-operative	1002:1015	arg1	anti-adhesion					1017:1029	post-operative anti-adhesion	1002:1029	post-operative anti-adhesion	1002:1029	Meanwhile, a rat injury sidewall-cecum abrasion model is developed to investigate the efficacy of these hydrogels in achieving post-operative anti-adhesion.
33812606	3	69	theme	sidewall-cecum	899:912	arg1	model					923:927	a rat injury sidewall-cecum abrasion model	886:927	a rat injury sidewall-cecum abrasion model	886:927	Meanwhile, a rat injury sidewall-cecum abrasion model is developed to investigate the efficacy of these hydrogels in achieving post-operative anti-adhesion.
33812606	3	69	theme	sidewall-cecum	899:912	arg1	Meanwhile					875:883	Meanwhile	875:883	Meanwhile	875:883	Meanwhile, a rat injury sidewall-cecum abrasion model is developed to investigate the efficacy of these hydrogels in achieving post-operative anti-adhesion.
33812606	1	70	theme	non-toxic	247:255	arg1	materials					289:297	novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials	188:297	novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA)	188:373	Herein, we fabricated novel self-healing, in situ injectable, biodegradable, and non-toxic hydrogels anti-adhesion barrier materials composed of N, O-carboxymethyl chitosan (N,O-CS) and oxidized dextran (ODA) without requiring any chemical cross-linking agent or external stimuli triggers for the prevention and treatment of post-operative peritoneal adhesions.
32474074	8	0	from	activities	1143:1152	arg1	cheese					1157:1162	cheese	1157:1162	cheese	1157:1162	Antimicrobial activities on cheese showed that gelatin nanofibers loaded by AIC (10% v/v), applied in the headspace, extended the shelf life of cheese from 4 weeks to 8 weeks.
32474074	7	1	theme	Staphylococcus	1066:1079	arg1	25923					1093:1097	Staphylococcus aureus ATCC 25923	1066:1097	Staphylococcus aureus ATCC 25923	1066:1097	Final product with AIC (2% v/v) showed significant antimicrobial activities against two food born bacteria including Staphylococcus aureus ATCC 25923 and Escherichia coli O157:H7.
32474074	1	2	theme	packing	148:154	arg1	solutions					130:138	Traditional solutions	118:138	Traditional solutions of food packing	118:154	Traditional solutions of food packing are limited for antimicrobial packaging materials that interact with the product to inhibit the growth of microorganisms.
32474074	4	3	theme	%	560:560	arg1	v/v					562:564	2-10% v/v	556:564	2-10% v/v	556:564	Gelatin electrospun fibers loaded by AIC (2-10% v/v) into 24% wt/v gelatin solutions was produced using electrospinning apparatus at 15 kV, 15 cm air gap and 0.2 mL/h flow rate.
32474074	4	3	theme	%	560:560	arg1	AIC					551:553	AIC	551:553	AIC (2-10% v/v)	551:565	Gelatin electrospun fibers loaded by AIC (2-10% v/v) into 24% wt/v gelatin solutions was produced using electrospinning apparatus at 15 kV, 15 cm air gap and 0.2 mL/h flow rate.
32474074	7	4	theme	aureus	1081:1086	arg1	25923					1093:1097	Staphylococcus aureus ATCC 25923	1066:1097	Staphylococcus aureus ATCC 25923	1066:1097	Final product with AIC (2% v/v) showed significant antimicrobial activities against two food born bacteria including Staphylococcus aureus ATCC 25923 and Escherichia coli O157:H7.
32474074	3	5	theme	supportive	487:496	arg1	sheet					507:511	supportive adhesive sheet	487:511	supportive adhesive sheet	487:511	Such adhesive composite was made of gelatin electrospun fibers loaded by AIC and supportive adhesive sheet.
32474074	0	6	theme	shelf	96:100	arg1	life					102:105	the shelf life	92:105	the shelf life of cheese	92:115	Fabrication of cellulose-based adhesive composite as an active packaging material to extend the shelf life of cheese.
32474074	8	7	theme	shelf	1259:1263	arg1	life					1265:1268	the shelf life	1255:1268	the shelf life of cheese	1255:1278	Antimicrobial activities on cheese showed that gelatin nanofibers loaded by AIC (10% v/v), applied in the headspace, extended the shelf life of cheese from 4 weeks to 8 weeks.
32474074	6	8	theme	analysis	939:946	arg1	means					888:892	means	888:892	means of spectral, physicochemical and rheological analysis	888:946	PSA was characterized in means of spectral, physicochemical and rheological analysis.
32474074	4	9	theme	air	660:662	arg1	gap					664:666	15 cm air gap	654:666	15 cm air gap	654:666	Gelatin electrospun fibers loaded by AIC (2-10% v/v) into 24% wt/v gelatin solutions was produced using electrospinning apparatus at 15 kV, 15 cm air gap and 0.2 mL/h flow rate.
32474074	1	10	theme	microorganisms	262:275	arg1	growth					252:257	the growth	248:257	the growth of microorganisms	248:275	Traditional solutions of food packing are limited for antimicrobial packaging materials that interact with the product to inhibit the growth of microorganisms.
32474074	4	11	theme	15 cm	654:658	arg1	gap					664:666	15 cm air gap	654:666	15 cm air gap	654:666	Gelatin electrospun fibers loaded by AIC (2-10% v/v) into 24% wt/v gelatin solutions was produced using electrospinning apparatus at 15 kV, 15 cm air gap and 0.2 mL/h flow rate.
32474074	7	12	theme	food	1037:1040	arg1	25923					1093:1097	Staphylococcus aureus ATCC 25923	1066:1097	Staphylococcus aureus ATCC 25923	1066:1097	Final product with AIC (2% v/v) showed significant antimicrobial activities against two food born bacteria including Staphylococcus aureus ATCC 25923 and Escherichia coli O157:H7.
32474074	7	12	theme	food	1037:1040	arg1	bacteria					1047:1054	two food born bacteria	1033:1054	two food born bacteria including Staphylococcus aureus ATCC 25923	1033:1097	Final product with AIC (2% v/v) showed significant antimicrobial activities against two food born bacteria including Staphylococcus aureus ATCC 25923 and Escherichia coli O157:H7.
32474074	4	13	theme	gelatin	581:587	arg1	solutions					589:597	24% wt/v gelatin solutions	572:597	24% wt/v gelatin solutions	572:597	Gelatin electrospun fibers loaded by AIC (2-10% v/v) into 24% wt/v gelatin solutions was produced using electrospinning apparatus at 15 kV, 15 cm air gap and 0.2 mL/h flow rate.
32474074	3	14	theme	Such	406:409	arg1	composite					420:428	Such adhesive composite	406:428	Such adhesive composite	406:428	Such adhesive composite was made of gelatin electrospun fibers loaded by AIC and supportive adhesive sheet.
32474074	4	15	theme	electrospinning	618:632	arg1	apparatus					634:642	electrospinning apparatus	618:642	electrospinning apparatus	618:642	Gelatin electrospun fibers loaded by AIC (2-10% v/v) into 24% wt/v gelatin solutions was produced using electrospinning apparatus at 15 kV, 15 cm air gap and 0.2 mL/h flow rate.
32474074	6	16	theme	rheological	927:937	arg1	analysis					939:946	spectral, physicochemical and rheological analysis	897:946	spectral, physicochemical and rheological analysis	897:946	PSA was characterized in means of spectral, physicochemical and rheological analysis.
32474074	7	17	theme	born	1042:1045	arg1	25923					1093:1097	Staphylococcus aureus ATCC 25923	1066:1097	Staphylococcus aureus ATCC 25923	1066:1097	Final product with AIC (2% v/v) showed significant antimicrobial activities against two food born bacteria including Staphylococcus aureus ATCC 25923 and Escherichia coli O157:H7.
32474074	7	17	theme	born	1042:1045	arg1	bacteria					1047:1054	two food born bacteria	1033:1054	two food born bacteria including Staphylococcus aureus ATCC 25923	1033:1097	Final product with AIC (2% v/v) showed significant antimicrobial activities against two food born bacteria including Staphylococcus aureus ATCC 25923 and Escherichia coli O157:H7.
32474074	7	18	theme	ATCC	1088:1091	arg1	25923					1093:1097	Staphylococcus aureus ATCC 25923	1066:1097	Staphylococcus aureus ATCC 25923	1066:1097	Final product with AIC (2% v/v) showed significant antimicrobial activities against two food born bacteria including Staphylococcus aureus ATCC 25923 and Escherichia coli O157:H7.
32474074	2	19	theme	allyl	363:367	arg1	AIC					385:387	AIC	385:387	AIC	385:387	In this work, adhesive composite was developed as an active packaging material using allyl isothiocyanate (AIC) in vapor phase.
32474074	2	19	theme	allyl	363:367	arg1	isothiocyanate					369:382	allyl isothiocyanate	363:382	allyl isothiocyanate (AIC)	363:388	In this work, adhesive composite was developed as an active packaging material using allyl isothiocyanate (AIC) in vapor phase.
32474074	8	20	theme	Antimicrobial	1129:1141	arg1	activities					1143:1152	Antimicrobial activities	1129:1152	Antimicrobial activities on cheese	1129:1162	Antimicrobial activities on cheese showed that gelatin nanofibers loaded by AIC (10% v/v), applied in the headspace, extended the shelf life of cheese from 4 weeks to 8 weeks.
32474074	1	21	theme	antimicrobial	172:184	arg1	materials					196:204	antimicrobial packaging materials	172:204	antimicrobial packaging materials that interact with the product to inhibit the growth of microorganisms	172:275	Traditional solutions of food packing are limited for antimicrobial packaging materials that interact with the product to inhibit the growth of microorganisms.
32474074	7	22	theme	Escherichia	1103:1113	arg1	O157					1120:1123	O157	1120:1123	Escherichia coli O157:H7	1103:1126	Final product with AIC (2% v/v) showed significant antimicrobial activities against two food born bacteria including Staphylococcus aureus ATCC 25923 and Escherichia coli O157:H7.
32474074	8	23	theme	cheese	1273:1278	arg1	life					1265:1268	the shelf life	1255:1268	the shelf life of cheese	1255:1278	Antimicrobial activities on cheese showed that gelatin nanofibers loaded by AIC (10% v/v), applied in the headspace, extended the shelf life of cheese from 4 weeks to 8 weeks.
32474074	7	24	with	product	955:961	arg1	v/v					976:978	2% v/v	973:978	2% v/v	973:978	Final product with AIC (2% v/v) showed significant antimicrobial activities against two food born bacteria including Staphylococcus aureus ATCC 25923 and Escherichia coli O157:H7.
32474074	7	24	with	product	955:961	arg1	AIC					968:970	AIC	968:970	AIC (2% v/v)	968:979	Final product with AIC (2% v/v) showed significant antimicrobial activities against two food born bacteria including Staphylococcus aureus ATCC 25923 and Escherichia coli O157:H7.
32474074	1	25	theme	packaging	186:194	arg1	materials					196:204	antimicrobial packaging materials	172:204	antimicrobial packaging materials that interact with the product to inhibit the growth of microorganisms	172:275	Traditional solutions of food packing are limited for antimicrobial packaging materials that interact with the product to inhibit the growth of microorganisms.
32474074	0	26	theme	adhesive	31:38	arg1	composite					40:48	cellulose-based adhesive composite	15:48	cellulose-based adhesive composite	15:48	Fabrication of cellulose-based adhesive composite as an active packaging material to extend the shelf life of cheese.
32474074	6	27	theme	physicochemical	907:921	arg1	analysis					939:946	spectral, physicochemical and rheological analysis	897:946	spectral, physicochemical and rheological analysis	897:946	PSA was characterized in means of spectral, physicochemical and rheological analysis.
32474074	7	28	theme	antimicrobial	1000:1012	arg1	activities					1014:1023	significant antimicrobial activities	988:1023	significant antimicrobial activities against two food born bacteria including Staphylococcus aureus ATCC 25923 and Escherichia coli O157:H7	988:1126	Final product with AIC (2% v/v) showed significant antimicrobial activities against two food born bacteria including Staphylococcus aureus ATCC 25923 and Escherichia coli O157:H7.
32474074	2	29	theme	adhesive	292:299	arg1	material					348:355	an active packaging material	328:355	an active packaging material using allyl isothiocyanate (AIC) in vapor phase	328:403	In this work, adhesive composite was developed as an active packaging material using allyl isothiocyanate (AIC) in vapor phase.
32474074	2	29	theme	adhesive	292:299	arg1	composite					301:309	adhesive composite	292:309	adhesive composite	292:309	In this work, adhesive composite was developed as an active packaging material using allyl isothiocyanate (AIC) in vapor phase.
32474074	3	30	theme	gelatin	442:448	arg1	fibers					462:467	gelatin electrospun fibers	442:467	gelatin electrospun fibers loaded by AIC and supportive adhesive sheet	442:511	Such adhesive composite was made of gelatin electrospun fibers loaded by AIC and supportive adhesive sheet.
32474074	0	31	theme	cellulose-based	15:29	arg1	composite					40:48	cellulose-based adhesive composite	15:48	cellulose-based adhesive composite	15:48	Fabrication of cellulose-based adhesive composite as an active packaging material to extend the shelf life of cheese.
32474074	0	32	theme	cheese	110:115	arg1	life					102:105	the shelf life	92:105	the shelf life of cheese	92:115	Fabrication of cellulose-based adhesive composite as an active packaging material to extend the shelf life of cheese.
32474074	2	33	theme	packaging	338:346	arg1	material					348:355	an active packaging material	328:355	an active packaging material using allyl isothiocyanate (AIC) in vapor phase	328:403	In this work, adhesive composite was developed as an active packaging material using allyl isothiocyanate (AIC) in vapor phase.
32474074	2	33	theme	packaging	338:346	arg1	composite					301:309	adhesive composite	292:309	adhesive composite	292:309	In this work, adhesive composite was developed as an active packaging material using allyl isothiocyanate (AIC) in vapor phase.
32474074	3	34	theme	electrospun	450:460	arg1	fibers					462:467	gelatin electrospun fibers	442:467	gelatin electrospun fibers loaded by AIC and supportive adhesive sheet	442:511	Such adhesive composite was made of gelatin electrospun fibers loaded by AIC and supportive adhesive sheet.
32474074	6	35	theme	spectral	897:904	arg1	analysis					939:946	spectral, physicochemical and rheological analysis	897:946	spectral, physicochemical and rheological analysis	897:946	PSA was characterized in means of spectral, physicochemical and rheological analysis.
32474074	3	36	theme	adhesive	411:418	arg1	composite					420:428	Such adhesive composite	406:428	Such adhesive composite	406:428	Such adhesive composite was made of gelatin electrospun fibers loaded by AIC and supportive adhesive sheet.
32474074	2	37	theme	vapor	393:397	arg1	phase					399:403	vapor phase	393:403	vapor phase	393:403	In this work, adhesive composite was developed as an active packaging material using allyl isothiocyanate (AIC) in vapor phase.
32474074	0	38	theme	composite	40:48	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of cellulose-based adhesive composite as an active packaging material	0:80	Fabrication of cellulose-based adhesive composite as an active packaging material to extend the shelf life of cheese.
32474074	4	39	theme	Gelatin	514:520	arg1	fibers					534:539	Gelatin electrospun fibers	514:539	Gelatin electrospun fibers loaded by AIC (2-10% v/v) into 24% wt/v gelatin solutions	514:597	Gelatin electrospun fibers loaded by AIC (2-10% v/v) into 24% wt/v gelatin solutions was produced using electrospinning apparatus at 15 kV, 15 cm air gap and 0.2 mL/h flow rate.
32474074	5	40	theme	methylbutyl	820:830	arg1	AA-MBA					853:858	AA-MBA	853:858	AA-MBA	853:858	This mat was supported by pressure-sensitive adhesive (PSA) made of hydroxyethyl cellulose backbone grafted by acrylic acid and methylbutyl acrylate [HEC-g-poly(AA-MBA)].
32474074	5	40	theme	methylbutyl	820:830	arg1	[HEC-g-poly					841:851	methylbutyl acrylate [HEC-g-poly	820:851	methylbutyl acrylate [HEC-g-poly(AA-MBA)]	820:860	This mat was supported by pressure-sensitive adhesive (PSA) made of hydroxyethyl cellulose backbone grafted by acrylic acid and methylbutyl acrylate [HEC-g-poly(AA-MBA)].
32474074	5	41	theme	hydroxyethyl	760:771	arg1	backbone					783:790	hydroxyethyl cellulose backbone	760:790	hydroxyethyl cellulose backbone grafted by acrylic acid and methylbutyl acrylate [HEC-g-poly(AA-MBA)]	760:860	This mat was supported by pressure-sensitive adhesive (PSA) made of hydroxyethyl cellulose backbone grafted by acrylic acid and methylbutyl acrylate [HEC-g-poly(AA-MBA)].
32474074	7	42	dep	O157	1120:1123	arg1	coli					1115:1118	Escherichia coli O157:H7	1103:1126	Escherichia coli O157:H7	1103:1126	Final product with AIC (2% v/v) showed significant antimicrobial activities against two food born bacteria including Staphylococcus aureus ATCC 25923 and Escherichia coli O157:H7.
32474074	7	42	dep	O157	1120:1123	arg1	H7					1125:1126	H7	1125:1126	Escherichia coli O157:H7	1103:1126	Final product with AIC (2% v/v) showed significant antimicrobial activities against two food born bacteria including Staphylococcus aureus ATCC 25923 and Escherichia coli O157:H7.
32474074	5	43	theme	acrylic	803:809	arg1	acid					811:814	acrylic acid	803:814	acrylic acid	803:814	This mat was supported by pressure-sensitive adhesive (PSA) made of hydroxyethyl cellulose backbone grafted by acrylic acid and methylbutyl acrylate [HEC-g-poly(AA-MBA)].
32474074	0	44	theme	active	56:61	arg1	material					73:80	an active packaging material	53:80	an active packaging material	53:80	Fabrication of cellulose-based adhesive composite as an active packaging material to extend the shelf life of cheese.
32474074	2	45	theme	active	331:336	arg1	material					348:355	an active packaging material	328:355	an active packaging material using allyl isothiocyanate (AIC) in vapor phase	328:403	In this work, adhesive composite was developed as an active packaging material using allyl isothiocyanate (AIC) in vapor phase.
32474074	2	45	theme	active	331:336	arg1	composite					301:309	adhesive composite	292:309	adhesive composite	292:309	In this work, adhesive composite was developed as an active packaging material using allyl isothiocyanate (AIC) in vapor phase.
32474074	7	46	theme	significant	988:998	arg1	activities					1014:1023	significant antimicrobial activities	988:1023	significant antimicrobial activities against two food born bacteria including Staphylococcus aureus ATCC 25923 and Escherichia coli O157:H7	988:1126	Final product with AIC (2% v/v) showed significant antimicrobial activities against two food born bacteria including Staphylococcus aureus ATCC 25923 and Escherichia coli O157:H7.
32474074	4	47	theme	electrospun	522:532	arg1	fibers					534:539	Gelatin electrospun fibers	514:539	Gelatin electrospun fibers loaded by AIC (2-10% v/v) into 24% wt/v gelatin solutions	514:597	Gelatin electrospun fibers loaded by AIC (2-10% v/v) into 24% wt/v gelatin solutions was produced using electrospinning apparatus at 15 kV, 15 cm air gap and 0.2 mL/h flow rate.
32474074	1	48	theme	Traditional	118:128	arg1	solutions					130:138	Traditional solutions	118:138	Traditional solutions of food packing	118:154	Traditional solutions of food packing are limited for antimicrobial packaging materials that interact with the product to inhibit the growth of microorganisms.
32474074	5	49	theme	acrylate	832:839	arg1	AA-MBA					853:858	AA-MBA	853:858	AA-MBA	853:858	This mat was supported by pressure-sensitive adhesive (PSA) made of hydroxyethyl cellulose backbone grafted by acrylic acid and methylbutyl acrylate [HEC-g-poly(AA-MBA)].
32474074	5	49	theme	acrylate	832:839	arg1	[HEC-g-poly					841:851	methylbutyl acrylate [HEC-g-poly	820:851	methylbutyl acrylate [HEC-g-poly(AA-MBA)]	820:860	This mat was supported by pressure-sensitive adhesive (PSA) made of hydroxyethyl cellulose backbone grafted by acrylic acid and methylbutyl acrylate [HEC-g-poly(AA-MBA)].
32474074	4	50	theme	24	572:573	arg1	%					574:574	%	574:574	%	574:574	Gelatin electrospun fibers loaded by AIC (2-10% v/v) into 24% wt/v gelatin solutions was produced using electrospinning apparatus at 15 kV, 15 cm air gap and 0.2 mL/h flow rate.
32474074	7	51	theme	%	974:974	arg1	v/v					976:978	2% v/v	973:978	2% v/v	973:978	Final product with AIC (2% v/v) showed significant antimicrobial activities against two food born bacteria including Staphylococcus aureus ATCC 25923 and Escherichia coli O157:H7.
32474074	7	51	theme	%	974:974	arg1	AIC					968:970	AIC	968:970	AIC (2% v/v)	968:979	Final product with AIC (2% v/v) showed significant antimicrobial activities against two food born bacteria including Staphylococcus aureus ATCC 25923 and Escherichia coli O157:H7.
32474074	4	52	theme	0.2 mL/h	672:679	arg1	rate					686:689	0.2 mL/h flow rate	672:689	0.2 mL/h flow rate	672:689	Gelatin electrospun fibers loaded by AIC (2-10% v/v) into 24% wt/v gelatin solutions was produced using electrospinning apparatus at 15 kV, 15 cm air gap and 0.2 mL/h flow rate.
32474074	8	53	theme	gelatin	1176:1182	arg1	nanofibers					1184:1193	gelatin nanofibers	1176:1193	gelatin nanofibers loaded by AIC (10% v/v), applied in the headspace,	1176:1244	Antimicrobial activities on cheese showed that gelatin nanofibers loaded by AIC (10% v/v), applied in the headspace, extended the shelf life of cheese from 4 weeks to 8 weeks.
32474074	4	54	theme	flow	681:684	arg1	rate					686:689	0.2 mL/h flow rate	672:689	0.2 mL/h flow rate	672:689	Gelatin electrospun fibers loaded by AIC (2-10% v/v) into 24% wt/v gelatin solutions was produced using electrospinning apparatus at 15 kV, 15 cm air gap and 0.2 mL/h flow rate.
32474074	8	55	theme	%	1212:1212	arg1	AIC					1205:1207	AIC	1205:1207	AIC (10% v/v)	1205:1217	Antimicrobial activities on cheese showed that gelatin nanofibers loaded by AIC (10% v/v), applied in the headspace, extended the shelf life of cheese from 4 weeks to 8 weeks.
32474074	8	55	theme	%	1212:1212	arg1	v/v					1214:1216	10% v/v	1210:1216	10% v/v	1210:1216	Antimicrobial activities on cheese showed that gelatin nanofibers loaded by AIC (10% v/v), applied in the headspace, extended the shelf life of cheese from 4 weeks to 8 weeks.
32474074	7	56	theme	Final	949:953	arg1	product					955:961	Final product	949:961	Final product with AIC (2% v/v)	949:979	Final product with AIC (2% v/v) showed significant antimicrobial activities against two food born bacteria including Staphylococcus aureus ATCC 25923 and Escherichia coli O157:H7.
32474074	3	57	theme	adhesive	498:505	arg1	sheet					507:511	supportive adhesive sheet	487:511	supportive adhesive sheet	487:511	Such adhesive composite was made of gelatin electrospun fibers loaded by AIC and supportive adhesive sheet.
32474074	4	58	theme	wt/v	576:579	arg1	solutions					589:597	24% wt/v gelatin solutions	572:597	24% wt/v gelatin solutions	572:597	Gelatin electrospun fibers loaded by AIC (2-10% v/v) into 24% wt/v gelatin solutions was produced using electrospinning apparatus at 15 kV, 15 cm air gap and 0.2 mL/h flow rate.
32474074	0	59	theme	packaging	63:71	arg1	material					73:80	an active packaging material	53:80	an active packaging material	53:80	Fabrication of cellulose-based adhesive composite as an active packaging material to extend the shelf life of cheese.
32474074	5	60	theme	cellulose	773:781	arg1	backbone					783:790	hydroxyethyl cellulose backbone	760:790	hydroxyethyl cellulose backbone grafted by acrylic acid and methylbutyl acrylate [HEC-g-poly(AA-MBA)]	760:860	This mat was supported by pressure-sensitive adhesive (PSA) made of hydroxyethyl cellulose backbone grafted by acrylic acid and methylbutyl acrylate [HEC-g-poly(AA-MBA)].
32474074	1	61	theme	food	143:146	arg1	packing					148:154	food packing	143:154	food packing	143:154	Traditional solutions of food packing are limited for antimicrobial packaging materials that interact with the product to inhibit the growth of microorganisms.
32474074	4	62	theme	%	574:574	arg1	solutions					589:597	24% wt/v gelatin solutions	572:597	24% wt/v gelatin solutions	572:597	Gelatin electrospun fibers loaded by AIC (2-10% v/v) into 24% wt/v gelatin solutions was produced using electrospinning apparatus at 15 kV, 15 cm air gap and 0.2 mL/h flow rate.
34823771	4	0	theme	Cu2+	538:541	arg1	capacities					524:533	The adsorption capacities	509:533	The adsorption capacities of Cu2+ and Fe3+	509:550	The adsorption capacities of Cu2+ and Fe3+ were 462 and 270 mg/g, lower than 934 mg/g of Pb2+.
34823771	4	0	theme	Cu2+	538:541	arg1	462					557:559	462	557:559	462	557:559	The adsorption capacities of Cu2+ and Fe3+ were 462 and 270 mg/g, lower than 934 mg/g of Pb2+.
34823771	8	1	theme	Trypoxylus	1064:1073	arg1	chitosan					1093:1100	the Trypoxylus dichotomus source chitosan	1060:1100	the Trypoxylus dichotomus source chitosan prepared by hydrothermal deacetylation	1060:1139	Therefore, the Trypoxylus dichotomus source chitosan prepared by hydrothermal deacetylation has potential applications in the adsorption of complex pollutants.
34823771	6	2	theme	single	756:761	arg1	pollutants					763:772	single pollutants	756:772	single pollutants	756:772	In addition, compared with single pollutants, the adsorption of sulfamethoxazole and Pb2+ can increase by 6% and 5% when they act as composite contaminants.
34823771	5	3	theme	tetracycline	621:632	arg1	capacity					659:666	tetracycline hydrochloride adsorption capacity	621:666	tetracycline hydrochloride adsorption capacity	621:666	Levofloxacin and tetracycline hydrochloride adsorption capacity were 26 and 22 mg/g, lower than 67 mg/g of sulfamethoxazole.
34823771	1	4	theme	multi-step	168:177	arg1	chitin					200:205	multi-step protein purification chitin	168:205	multi-step protein purification chitin based on Trypoxylus dichotomus	168:236	Chitosan was prepared by hydrothermal deacetylation from multi-step protein purification chitin based on Trypoxylus dichotomus, for treating heavy metals and antibiotics.
34823771	4	5	theme	Pb2+	598:601	arg1	934 mg/g					586:593	934 mg/g	586:593	934 mg/g of Pb2+	586:601	The adsorption capacities of Cu2+ and Fe3+ were 462 and 270 mg/g, lower than 934 mg/g of Pb2+.
34823771	4	6	theme	Fe3+	547:550	arg1	capacities					524:533	The adsorption capacities	509:533	The adsorption capacities of Cu2+ and Fe3+	509:550	The adsorption capacities of Cu2+ and Fe3+ were 462 and 270 mg/g, lower than 934 mg/g of Pb2+.
34823771	4	6	theme	Fe3+	547:550	arg1	462					557:559	462	557:559	462	557:559	The adsorption capacities of Cu2+ and Fe3+ were 462 and 270 mg/g, lower than 934 mg/g of Pb2+.
34823771	8	7	theme	source	1086:1091	arg1	chitosan					1093:1100	the Trypoxylus dichotomus source chitosan	1060:1100	the Trypoxylus dichotomus source chitosan prepared by hydrothermal deacetylation	1060:1139	Therefore, the Trypoxylus dichotomus source chitosan prepared by hydrothermal deacetylation has potential applications in the adsorption of complex pollutants.
34823771	5	8	theme	hydrochloride	634:646	arg1	capacity					659:666	tetracycline hydrochloride adsorption capacity	621:666	tetracycline hydrochloride adsorption capacity	621:666	Levofloxacin and tetracycline hydrochloride adsorption capacity were 26 and 22 mg/g, lower than 67 mg/g of sulfamethoxazole.
34823771	7	9	theme	adsorption	890:899	arg1	procedure					901:909	The adsorption procedure	886:909	The adsorption procedure	886:909	The adsorption procedure can be well described by pseudo-second-order kinetics and Langmuir isothermal model, indicating it a homogeneous monolayer chemisorption.
34823771	7	10	theme	homogeneous	1012:1022	arg1	chemisorption					1034:1046	a homogeneous monolayer chemisorption	1010:1046	it a homogeneous monolayer chemisorption	1007:1046	The adsorption procedure can be well described by pseudo-second-order kinetics and Langmuir isothermal model, indicating it a homogeneous monolayer chemisorption.
34823771	4	11	theme	adsorption	513:522	arg1	capacities					524:533	The adsorption capacities	509:533	The adsorption capacities of Cu2+ and Fe3+	509:550	The adsorption capacities of Cu2+ and Fe3+ were 462 and 270 mg/g, lower than 934 mg/g of Pb2+.
34823771	4	11	theme	adsorption	513:522	arg1	462					557:559	462	557:559	462	557:559	The adsorption capacities of Cu2+ and Fe3+ were 462 and 270 mg/g, lower than 934 mg/g of Pb2+.
34823771	5	12	theme	adsorption	648:657	arg1	capacity					659:666	tetracycline hydrochloride adsorption capacity	621:666	tetracycline hydrochloride adsorption capacity	621:666	Levofloxacin and tetracycline hydrochloride adsorption capacity were 26 and 22 mg/g, lower than 67 mg/g of sulfamethoxazole.
34823771	2	13	theme	deacetylation	303:315	arg1	degree					317:322	higher deacetylation degree	296:322	higher deacetylation degree	296:322	Chitosan with higher deacetylation degree and lower molecular weight were synthesized.
34823771	3	14	theme	adult	373:377	arg1	chitosan					379:386	The adult chitosan	369:386	The adult chitosan	369:386	The adult chitosan was composed of nanofibers arranged more evenly, showing higher yield, thermal stabilities and antimicrobial properties.
34823771	2	15	theme	higher	296:301	arg1	degree					317:322	higher deacetylation degree	296:322	higher deacetylation degree	296:322	Chitosan with higher deacetylation degree and lower molecular weight were synthesized.
34823771	1	16	theme	protein	179:185	arg1	chitin					200:205	multi-step protein purification chitin	168:205	multi-step protein purification chitin based on Trypoxylus dichotomus	168:236	Chitosan was prepared by hydrothermal deacetylation from multi-step protein purification chitin based on Trypoxylus dichotomus, for treating heavy metals and antibiotics.
34823771	1	17	theme	purification	187:198	arg1	chitin					200:205	multi-step protein purification chitin	168:205	multi-step protein purification chitin based on Trypoxylus dichotomus	168:236	Chitosan was prepared by hydrothermal deacetylation from multi-step protein purification chitin based on Trypoxylus dichotomus, for treating heavy metals and antibiotics.
34823771	7	18	theme	isothermal	978:987	arg1	model					989:993	Langmuir isothermal model	969:993	Langmuir isothermal model	969:993	The adsorption procedure can be well described by pseudo-second-order kinetics and Langmuir isothermal model, indicating it a homogeneous monolayer chemisorption.
34823771	8	19	theme	hydrothermal	1114:1125	arg1	deacetylation					1127:1139	hydrothermal deacetylation	1114:1139	hydrothermal deacetylation	1114:1139	Therefore, the Trypoxylus dichotomus source chitosan prepared by hydrothermal deacetylation has potential applications in the adsorption of complex pollutants.
34823771	7	20	theme	Langmuir	969:976	arg1	model					989:993	Langmuir isothermal model	969:993	Langmuir isothermal model	969:993	The adsorption procedure can be well described by pseudo-second-order kinetics and Langmuir isothermal model, indicating it a homogeneous monolayer chemisorption.
34823771	0	21	theme	Adsorption	0:9	arg1	properties					11:20	Adsorption properties	0:20	Adsorption properties of heavy metals and antibiotics	0:52	Adsorption properties of heavy metals and antibiotics by chitosan from larvae and adult Trypoxylus dichotomus.
34823771	7	22	theme	pseudo-second-order	936:954	arg1	kinetics					956:963	pseudo-second-order kinetics	936:963	pseudo-second-order kinetics	936:963	The adsorption procedure can be well described by pseudo-second-order kinetics and Langmuir isothermal model, indicating it a homogeneous monolayer chemisorption.
34823771	2	23	theme	molecular	334:342	arg1	weight					344:349	lower molecular weight	328:349	lower molecular weight	328:349	Chitosan with higher deacetylation degree and lower molecular weight were synthesized.
34823771	7	24	dep	it	1007:1008	arg1	chemisorption					1034:1046	a homogeneous monolayer chemisorption	1010:1046	it a homogeneous monolayer chemisorption	1007:1046	The adsorption procedure can be well described by pseudo-second-order kinetics and Langmuir isothermal model, indicating it a homogeneous monolayer chemisorption.
34823771	0	25	theme	heavy	25:29	arg1	metals					31:36	heavy metals	25:36	heavy metals	25:36	Adsorption properties of heavy metals and antibiotics by chitosan from larvae and adult Trypoxylus dichotomus.
34823771	6	26	theme	Pb2+	814:817	arg1	adsorption					779:788	the adsorption	775:788	the adsorption of sulfamethoxazole and Pb2+	775:817	In addition, compared with single pollutants, the adsorption of sulfamethoxazole and Pb2+ can increase by 6% and 5% when they act as composite contaminants.
34823771	2	27	theme	lower	328:332	arg1	weight					344:349	lower molecular weight	328:349	lower molecular weight	328:349	Chitosan with higher deacetylation degree and lower molecular weight were synthesized.
34823771	0	28	dep	chitosan	57:64	arg1	dichotomus					99:108	Trypoxylus dichotomus	88:108	Trypoxylus dichotomus	88:108	Adsorption properties of heavy metals and antibiotics by chitosan from larvae and adult Trypoxylus dichotomus.
34823771	0	29	from	larvae	71:76	arg1	chitosan					57:64	chitosan	57:64	chitosan	57:64	Adsorption properties of heavy metals and antibiotics by chitosan from larvae and adult Trypoxylus dichotomus.
34823771	8	30	dep	Trypoxylus	1064:1073	arg1	dichotomus					1075:1084	dichotomus	1075:1084	dichotomus	1075:1084	Therefore, the Trypoxylus dichotomus source chitosan prepared by hydrothermal deacetylation has potential applications in the adsorption of complex pollutants.
34823771	3	31	theme	thermal	459:465	arg1	yield					452:456	higher yield	445:456	higher yield	445:456	The adult chitosan was composed of nanofibers arranged more evenly, showing higher yield, thermal stabilities and antimicrobial properties.
34823771	3	31	theme	thermal	459:465	arg1	stabilities					467:477	thermal stabilities	459:477	thermal stabilities	459:477	The adult chitosan was composed of nanofibers arranged more evenly, showing higher yield, thermal stabilities and antimicrobial properties.
34823771	0	32	theme	metals	31:36	arg1	properties					11:20	Adsorption properties	0:20	Adsorption properties of heavy metals and antibiotics	0:52	Adsorption properties of heavy metals and antibiotics by chitosan from larvae and adult Trypoxylus dichotomus.
34823771	6	33	theme	composite	862:870	arg1	they					850:853	they	850:853	they	850:853	In addition, compared with single pollutants, the adsorption of sulfamethoxazole and Pb2+ can increase by 6% and 5% when they act as composite contaminants.
34823771	6	33	theme	composite	862:870	arg1	contaminants					872:883	composite contaminants	862:883	composite contaminants	862:883	In addition, compared with single pollutants, the adsorption of sulfamethoxazole and Pb2+ can increase by 6% and 5% when they act as composite contaminants.
34823771	3	34	theme	antimicrobial	483:495	arg1	yield					452:456	higher yield	445:456	higher yield	445:456	The adult chitosan was composed of nanofibers arranged more evenly, showing higher yield, thermal stabilities and antimicrobial properties.
34823771	3	34	theme	antimicrobial	483:495	arg1	properties					497:506	antimicrobial properties	483:506	antimicrobial properties	483:506	The adult chitosan was composed of nanofibers arranged more evenly, showing higher yield, thermal stabilities and antimicrobial properties.
34823771	6	35	theme	sulfamethoxazole	793:808	arg1	adsorption					779:788	the adsorption	775:788	the adsorption of sulfamethoxazole and Pb2+	775:817	In addition, compared with single pollutants, the adsorption of sulfamethoxazole and Pb2+ can increase by 6% and 5% when they act as composite contaminants.
34823771	0	36	theme	antibiotics	42:52	arg1	properties					11:20	Adsorption properties	0:20	Adsorption properties of heavy metals and antibiotics	0:52	Adsorption properties of heavy metals and antibiotics by chitosan from larvae and adult Trypoxylus dichotomus.
34823771	2	37	with	Chitosan	282:289	arg1	degree					317:322	higher deacetylation degree	296:322	higher deacetylation degree	296:322	Chitosan with higher deacetylation degree and lower molecular weight were synthesized.
34823771	2	37	with	Chitosan	282:289	arg1	weight					344:349	lower molecular weight	328:349	lower molecular weight	328:349	Chitosan with higher deacetylation degree and lower molecular weight were synthesized.
34823771	5	38	theme	sulfamethoxazole	711:726	arg1	67 mg/g					700:706	67 mg/g	700:706	67 mg/g of sulfamethoxazole	700:726	Levofloxacin and tetracycline hydrochloride adsorption capacity were 26 and 22 mg/g, lower than 67 mg/g of sulfamethoxazole.
34823771	8	39	contain	has	1141:1143	arg2	applications					1155:1166	potential applications	1145:1166	potential applications	1145:1166	Therefore, the Trypoxylus dichotomus source chitosan prepared by hydrothermal deacetylation has potential applications in the adsorption of complex pollutants.
34823771	8	39	contain	has	1141:1143	arg1	chitosan					1093:1100	the Trypoxylus dichotomus source chitosan	1060:1100	the Trypoxylus dichotomus source chitosan prepared by hydrothermal deacetylation	1060:1139	Therefore, the Trypoxylus dichotomus source chitosan prepared by hydrothermal deacetylation has potential applications in the adsorption of complex pollutants.
34823771	1	40	from	chitin	200:205	arg1	deacetylation					149:161	hydrothermal deacetylation	136:161	hydrothermal deacetylation from multi-step protein purification chitin based on Trypoxylus dichotomus	136:236	Chitosan was prepared by hydrothermal deacetylation from multi-step protein purification chitin based on Trypoxylus dichotomus, for treating heavy metals and antibiotics.
34823771	8	41	theme	pollutants	1197:1206	arg1	adsorption					1175:1184	the adsorption	1171:1184	the adsorption of complex pollutants	1171:1206	Therefore, the Trypoxylus dichotomus source chitosan prepared by hydrothermal deacetylation has potential applications in the adsorption of complex pollutants.
34823771	0	42	dep	properties	11:20	arg1	chitosan					57:64	chitosan	57:64	chitosan	57:64	Adsorption properties of heavy metals and antibiotics by chitosan from larvae and adult Trypoxylus dichotomus.
34823771	0	42	dep	properties	11:20	arg1	adult					82:86	adult	82:86	adult	82:86	Adsorption properties of heavy metals and antibiotics by chitosan from larvae and adult Trypoxylus dichotomus.
34823771	7	43	theme	monolayer	1024:1032	arg1	chemisorption					1034:1046	a homogeneous monolayer chemisorption	1010:1046	it a homogeneous monolayer chemisorption	1007:1046	The adsorption procedure can be well described by pseudo-second-order kinetics and Langmuir isothermal model, indicating it a homogeneous monolayer chemisorption.
34823771	1	44	theme	hydrothermal	136:147	arg1	deacetylation					149:161	hydrothermal deacetylation	136:161	hydrothermal deacetylation from multi-step protein purification chitin based on Trypoxylus dichotomus	136:236	Chitosan was prepared by hydrothermal deacetylation from multi-step protein purification chitin based on Trypoxylus dichotomus, for treating heavy metals and antibiotics.
34823771	1	45	theme	heavy	252:256	arg1	metals					258:263	heavy metals	252:263	heavy metals	252:263	Chitosan was prepared by hydrothermal deacetylation from multi-step protein purification chitin based on Trypoxylus dichotomus, for treating heavy metals and antibiotics.
34823771	8	46	theme	complex	1189:1195	arg1	pollutants					1197:1206	complex pollutants	1189:1206	complex pollutants	1189:1206	Therefore, the Trypoxylus dichotomus source chitosan prepared by hydrothermal deacetylation has potential applications in the adsorption of complex pollutants.
34823771	3	47	theme	higher	445:450	arg1	yield					452:456	higher yield	445:456	higher yield	445:456	The adult chitosan was composed of nanofibers arranged more evenly, showing higher yield, thermal stabilities and antimicrobial properties.
34823771	3	47	theme	higher	445:450	arg1	stabilities					467:477	thermal stabilities	459:477	thermal stabilities	459:477	The adult chitosan was composed of nanofibers arranged more evenly, showing higher yield, thermal stabilities and antimicrobial properties.
34823771	3	47	theme	higher	445:450	arg1	properties					497:506	antimicrobial properties	483:506	antimicrobial properties	483:506	The adult chitosan was composed of nanofibers arranged more evenly, showing higher yield, thermal stabilities and antimicrobial properties.
34823771	8	48	theme	potential	1145:1153	arg1	applications					1155:1166	potential applications	1145:1166	potential applications	1145:1166	Therefore, the Trypoxylus dichotomus source chitosan prepared by hydrothermal deacetylation has potential applications in the adsorption of complex pollutants.
31923516	7	0	theme	particles	1153:1161	arg1	existence					1110:1118	the existence	1106:1118	the existence of 165.52 to 255.17 nm sized nHA particles and 167.35 to 334.69 nm sized nBG particles	1106:1205	The TEM analysis revealed the existence of 165.52 to 255.17 nm sized nHA particles and 167.35 to 334.69 nm sized nBG particles.
31923516	2	1	theme	comparative	366:376	arg1	assessment					378:387	a comparative assessment	364:387	a comparative assessment of chitosan-nanohydroxyapatite (CTS-nHA) and chitosan-nano-bioglass (CTS-nBG) scaffolds	364:475	In this paper, we described a comparative assessment of chitosan-nanohydroxyapatite (CTS-nHA) and chitosan-nano-bioglass (CTS-nBG) scaffolds.
31923516	7	2	theme	sized	1143:1147	arg1	particles					1153:1161	165.52 to 255.17 nm sized nHA particles	1123:1161	165.52 to 255.17 nm sized nHA particles	1123:1161	The TEM analysis revealed the existence of 165.52 to 255.17 nm sized nHA particles and 167.35 to 334.69 nm sized nBG particles.
31923516	5	3	theme	physiochemical	673:686	arg1	analysis					706:713	The physiochemical and morphological analysis	669:713	The physiochemical and morphological analysis of all samples	669:728	The physiochemical and morphological analysis of all samples has been performed through X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
31923516	11	4	theme	good	1621:1624	arg1	compatibility					1626:1638	good compatibility	1621:1638	good compatibility	1621:1638	The cell culture experiment revealed that fabricated scaffolds had good compatibility with fibroblast cells (L929, ATCC) and MG-63 which are able to adhere, proliferate, and migrate through the porous structure.
31923516	5	5	theme	morphological	692:704	arg1	analysis					706:713	The physiochemical and morphological analysis	669:713	The physiochemical and morphological analysis of all samples	669:728	The physiochemical and morphological analysis of all samples has been performed through X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
31923516	13	6	theme	cell	2012:2015	arg1	growth					2017:2022	cell growth	2012:2022	cell growth	2012:2022	The scaffolds with chitosan, bioglass, and hydroxyapatite have better biomechanical characteristics and allow cell growth.
31923516	1	7	theme	ceramic	223:229	arg1	bioglass					265:272	bioglass	265:272	bioglass	265:272	The thought of biodegradable organic-inorganic composites composed of natural polymer chitosan and ceramic nanoparticles (hydroxyapatite and bioglass) can be considered as a solution for hard tissue engineering.
31923516	1	7	theme	ceramic	223:229	arg1	hydroxyapatite					246:259	hydroxyapatite	246:259	hydroxyapatite	246:259	The thought of biodegradable organic-inorganic composites composed of natural polymer chitosan and ceramic nanoparticles (hydroxyapatite and bioglass) can be considered as a solution for hard tissue engineering.
31923516	1	7	theme	ceramic	223:229	arg1	nanoparticles					231:243	ceramic nanoparticles	223:243	ceramic nanoparticles (hydroxyapatite and bioglass)	223:273	The thought of biodegradable organic-inorganic composites composed of natural polymer chitosan and ceramic nanoparticles (hydroxyapatite and bioglass) can be considered as a solution for hard tissue engineering.
31923516	6	8	theme	shaped	999:1004	arg1	particles					1010:1018	spherically shaped nHA particles	987:1018	spherically shaped nHA particles of 4.20 μm	987:1029	The SEM image confirmed the presence of spherically shaped nHA particles of 4.20 μm and irregularly shaped nBG particles of 6.89 μm.
31923516	5	9	theme	powder	763:768	arg1	XRD					783:785	XRD	783:785	XRD	783:785	The physiochemical and morphological analysis of all samples has been performed through X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
31923516	5	9	theme	powder	763:768	arg1	diffraction					770:780	X-ray powder diffraction	757:780	X-ray powder diffraction (XRD)	757:786	The physiochemical and morphological analysis of all samples has been performed through X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
31923516	4	10	dep	scaffolds	635:643	arg1	shown					645:649	shown	645:649	scaffolds shown porous structure	635:666	The freeze-dried composite based CTS-nHA and CTS-nBG scaffolds shown porous structure.
31923516	8	11	theme	interconnected	1237:1250	arg1	structure					1252:1260	the interconnected structure	1233:1260	the interconnected structure of CTS-nHA and CTS-nBG nanocomposites	1233:1298	TEM analysis also showed the interconnected structure of CTS-nHA and CTS-nBG nanocomposites.
31923516	1	12	theme	organic-inorganic	153:169	arg1	composites					171:180	biodegradable organic-inorganic composites	139:180	biodegradable organic-inorganic composites composed of natural polymer chitosan and ceramic nanoparticles (hydroxyapatite and bioglass)	139:273	The thought of biodegradable organic-inorganic composites composed of natural polymer chitosan and ceramic nanoparticles (hydroxyapatite and bioglass) can be considered as a solution for hard tissue engineering.
31923516	6	13	theme	nBG	1054:1056	arg1	particles					1058:1066	irregularly shaped nBG particles	1035:1066	irregularly shaped nBG particles of 6.89 μm	1035:1077	The SEM image confirmed the presence of spherically shaped nHA particles of 4.20 μm and irregularly shaped nBG particles of 6.89 μm.
31923516	0	14	theme	CTS-nBG	70:76	arg1	scaffolds					78:86	freeze-dried CTS-nHA and CTS-nBG scaffolds	45:86	freeze-dried CTS-nHA and CTS-nBG scaffolds	45:86	Fabrication and in-vitro biocompatibility of freeze-dried CTS-nHA and CTS-nBG scaffolds for bone regeneration applications.
31923516	5	15	theme	scanning	867:874	arg1	SEM					897:899	SEM	897:899	SEM	897:899	The physiochemical and morphological analysis of all samples has been performed through X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
31923516	5	15	theme	scanning	867:874	arg1	microscopy					885:894	scanning electron microscopy	867:894	scanning electron microscopy (SEM)	867:900	The physiochemical and morphological analysis of all samples has been performed through X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
31923516	6	16	theme	particles	1058:1066	arg1	presence					975:982	the presence	971:982	the presence of spherically shaped nHA particles of 4.20 μm and irregularly shaped nBG particles of 6.89 μm	971:1077	The SEM image confirmed the presence of spherically shaped nHA particles of 4.20 μm and irregularly shaped nBG particles of 6.89 μm.
31923516	9	17	theme	good	1378:1381	arg1	behavior					1398:1405	good mineralization behavior	1378:1405	good mineralization behavior in simulated body fluid (SBF)	1378:1435	After seven days' incubation period, the CTS-nHA and CTS-nBG scaffolds shown good mineralization behavior in simulated body fluid (SBF).
31923516	11	18	theme	culture	1563:1569	arg1	experiment					1571:1580	The cell culture experiment	1554:1580	The cell culture experiment	1554:1580	The cell culture experiment revealed that fabricated scaffolds had good compatibility with fibroblast cells (L929, ATCC) and MG-63 which are able to adhere, proliferate, and migrate through the porous structure.
31923516	5	19	theme	samples	722:728	arg1	analysis					706:713	The physiochemical and morphological analysis	669:713	The physiochemical and morphological analysis of all samples	669:728	The physiochemical and morphological analysis of all samples has been performed through X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
31923516	6	20	theme	4.20 μm	1023:1029	arg1	particles					1010:1018	spherically shaped nHA particles	987:1018	spherically shaped nHA particles of 4.20 μm	987:1029	The SEM image confirmed the presence of spherically shaped nHA particles of 4.20 μm and irregularly shaped nBG particles of 6.89 μm.
31923516	6	20	theme	4.20 μm	1023:1029	arg1	particles					1058:1066	irregularly shaped nBG particles	1035:1066	irregularly shaped nBG particles of 6.89 μm	1035:1077	The SEM image confirmed the presence of spherically shaped nHA particles of 4.20 μm and irregularly shaped nBG particles of 6.89 μm.
31923516	13	21	contain	have	1960:1963	arg2	characteristics					1986:2000	better biomechanical characteristics	1965:2000	better biomechanical characteristics	1965:2000	The scaffolds with chitosan, bioglass, and hydroxyapatite have better biomechanical characteristics and allow cell growth.
31923516	13	21	contain	have	1960:1963	arg1	scaffolds					1906:1914	The scaffolds	1902:1914	The scaffolds with chitosan, bioglass, and hydroxyapatite	1902:1958	The scaffolds with chitosan, bioglass, and hydroxyapatite have better biomechanical characteristics and allow cell growth.
31923516	10	22	theme	enhanced	1470:1477	arg1	strength					1491:1498	enhanced compressive strength	1470:1498	enhanced compressive strength	1470:1498	The CTS-nHA scaffolds exhibited enhanced compressive strength and elastic modulus compared with the CTS-nBG sample.
31923516	0	23	theme	regeneration	97:108	arg1	applications					110:121	bone regeneration applications	92:121	bone regeneration applications	92:121	Fabrication and in-vitro biocompatibility of freeze-dried CTS-nHA and CTS-nBG scaffolds for bone regeneration applications.
31923516	14	24	theme	various	2082:2088	arg1	applications					2114:2125	various hard tissue engineering applications	2082:2125	various hard tissue engineering applications such as bone regeneration	2082:2151	Therefore, these scaffolds can be perfect candidates for various hard tissue engineering applications such as bone regeneration.
31923516	14	24	theme	various	2082:2088	arg1	regeneration					2140:2151	bone regeneration	2135:2151	bone regeneration	2135:2151	Therefore, these scaffolds can be perfect candidates for various hard tissue engineering applications such as bone regeneration.
31923516	6	25	theme	particles	1010:1018	arg1	presence					975:982	the presence	971:982	the presence of spherically shaped nHA particles of 4.20 μm and irregularly shaped nBG particles of 6.89 μm	971:1077	The SEM image confirmed the presence of spherically shaped nHA particles of 4.20 μm and irregularly shaped nBG particles of 6.89 μm.
31923516	2	26	dep	chitosan-nanohydroxyapatite	392:418	arg1	CTS-nBG					458:464	CTS-nBG	458:464	CTS-nBG	458:464	In this paper, we described a comparative assessment of chitosan-nanohydroxyapatite (CTS-nHA) and chitosan-nano-bioglass (CTS-nBG) scaffolds.
31923516	2	26	dep	chitosan-nanohydroxyapatite	392:418	arg1	CTS-nHA					421:427	CTS-nHA	421:427	CTS-nHA	421:427	In this paper, we described a comparative assessment of chitosan-nanohydroxyapatite (CTS-nHA) and chitosan-nano-bioglass (CTS-nBG) scaffolds.
31923516	5	27	theme	Fourier-transform	789:805	arg1	spectroscopy					816:827	Fourier-transform infrared spectroscopy	789:827	Fourier-transform infrared spectroscopy (FTIR)	789:834	The physiochemical and morphological analysis of all samples has been performed through X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
31923516	5	27	theme	Fourier-transform	789:805	arg1	FTIR					830:833	FTIR	830:833	FTIR	830:833	The physiochemical and morphological analysis of all samples has been performed through X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
31923516	10	28	theme	elastic	1504:1510	arg1	modulus					1512:1518	elastic modulus	1504:1518	elastic modulus	1504:1518	The CTS-nHA scaffolds exhibited enhanced compressive strength and elastic modulus compared with the CTS-nBG sample.
31923516	0	29	theme	scaffolds	78:86	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and in-vitro biocompatibility of freeze-dried CTS-nHA and CTS-nBG scaffolds for bone regeneration applications.
31923516	0	29	theme	scaffolds	78:86	arg1	biocompatibility					25:40	in-vitro biocompatibility	16:40	in-vitro biocompatibility	16:40	Fabrication and in-vitro biocompatibility of freeze-dried CTS-nHA and CTS-nBG scaffolds for bone regeneration applications.
31923516	3	30	theme	hydroxyapatite	507:520	arg1	dispersion					482:491	The dispersion	478:491	The dispersion of nanoscaled hydroxyapatite (nHA) and bioglass (nBG) in chitosan	478:557	The dispersion of nanoscaled hydroxyapatite (nHA) and bioglass (nBG) in chitosan remained satisfactory.
31923516	7	31	theme	nBG	1193:1195	arg1	particles					1197:1205	167.35 to 334.69 nm sized nBG particles	1167:1205	167.35 to 334.69 nm sized nBG particles	1167:1205	The TEM analysis revealed the existence of 165.52 to 255.17 nm sized nHA particles and 167.35 to 334.69 nm sized nBG particles.
31923516	1	32	theme	tissue	316:321	arg1	engineering					323:333	hard tissue engineering	311:333	hard tissue engineering	311:333	The thought of biodegradable organic-inorganic composites composed of natural polymer chitosan and ceramic nanoparticles (hydroxyapatite and bioglass) can be considered as a solution for hard tissue engineering.
31923516	8	33	dep	CTS-nHA	1265:1271	arg1	nanocomposites					1285:1298	nanocomposites	1285:1298	nanocomposites	1285:1298	TEM analysis also showed the interconnected structure of CTS-nHA and CTS-nBG nanocomposites.
31923516	4	34	theme	composite	599:607	arg1	CTS-nHA					615:621	The freeze-dried composite based CTS-nHA	582:621	The freeze-dried composite based CTS-nHA	582:621	The freeze-dried composite based CTS-nHA and CTS-nBG scaffolds shown porous structure.
31923516	14	35	theme	bone	2135:2138	arg1	regeneration					2140:2151	bone regeneration	2135:2151	bone regeneration	2135:2151	Therefore, these scaffolds can be perfect candidates for various hard tissue engineering applications such as bone regeneration.
31923516	0	36	theme	in-vitro	16:23	arg1	biocompatibility					25:40	in-vitro biocompatibility	16:40	in-vitro biocompatibility	16:40	Fabrication and in-vitro biocompatibility of freeze-dried CTS-nHA and CTS-nBG scaffolds for bone regeneration applications.
31923516	0	37	theme	CTS-nHA	58:64	arg1	scaffolds					78:86	freeze-dried CTS-nHA and CTS-nBG scaffolds	45:86	freeze-dried CTS-nHA and CTS-nBG scaffolds	45:86	Fabrication and in-vitro biocompatibility of freeze-dried CTS-nHA and CTS-nBG scaffolds for bone regeneration applications.
31923516	9	38	theme	simulated	1410:1418	arg1	SBF					1432:1434	SBF	1432:1434	SBF	1432:1434	After seven days' incubation period, the CTS-nHA and CTS-nBG scaffolds shown good mineralization behavior in simulated body fluid (SBF).
31923516	9	38	theme	simulated	1410:1418	arg1	fluid					1425:1429	simulated body fluid	1410:1429	simulated body fluid (SBF)	1410:1435	After seven days' incubation period, the CTS-nHA and CTS-nBG scaffolds shown good mineralization behavior in simulated body fluid (SBF).
31923516	12	39	theme	apatite	1883:1889	arg1	formation					1891:1899	the apatite formation	1879:1899	the apatite formation	1879:1899	All the obtained results clearly recommend that pre-loaded hydroxyapatite and bioglass nanoparticles can enhance the apatite formation.
31923516	14	40	theme	engineering	2102:2112	arg1	applications					2114:2125	various hard tissue engineering applications	2082:2125	various hard tissue engineering applications such as bone regeneration	2082:2151	Therefore, these scaffolds can be perfect candidates for various hard tissue engineering applications such as bone regeneration.
31923516	14	40	theme	engineering	2102:2112	arg1	regeneration					2140:2151	bone regeneration	2135:2151	bone regeneration	2135:2151	Therefore, these scaffolds can be perfect candidates for various hard tissue engineering applications such as bone regeneration.
31923516	11	41	theme	fibroblast	1645:1654	arg1	cells					1656:1660	fibroblast cells	1645:1660	fibroblast cells (L929, ATCC)	1645:1673	The cell culture experiment revealed that fabricated scaffolds had good compatibility with fibroblast cells (L929, ATCC) and MG-63 which are able to adhere, proliferate, and migrate through the porous structure.
31923516	12	42	theme	hydroxyapatite	1825:1838	arg1	nanoparticles					1853:1865	pre-loaded hydroxyapatite and bioglass nanoparticles	1814:1865	pre-loaded hydroxyapatite and bioglass nanoparticles	1814:1865	All the obtained results clearly recommend that pre-loaded hydroxyapatite and bioglass nanoparticles can enhance the apatite formation.
31923516	3	43	theme	nanoscaled	496:505	arg1	nHA					523:525	nHA	523:525	nHA	523:525	The dispersion of nanoscaled hydroxyapatite (nHA) and bioglass (nBG) in chitosan remained satisfactory.
31923516	3	43	theme	nanoscaled	496:505	arg1	hydroxyapatite					507:520	nanoscaled hydroxyapatite	496:520	nanoscaled hydroxyapatite (nHA)	496:526	The dispersion of nanoscaled hydroxyapatite (nHA) and bioglass (nBG) in chitosan remained satisfactory.
31923516	1	44	dep	nanoparticles	231:243	arg1	bioglass					265:272	bioglass	265:272	bioglass	265:272	The thought of biodegradable organic-inorganic composites composed of natural polymer chitosan and ceramic nanoparticles (hydroxyapatite and bioglass) can be considered as a solution for hard tissue engineering.
31923516	1	44	dep	nanoparticles	231:243	arg1	hydroxyapatite					246:259	hydroxyapatite	246:259	hydroxyapatite	246:259	The thought of biodegradable organic-inorganic composites composed of natural polymer chitosan and ceramic nanoparticles (hydroxyapatite and bioglass) can be considered as a solution for hard tissue engineering.
31923516	1	44	dep	nanoparticles	231:243	arg1	nanoparticles					231:243	ceramic nanoparticles	223:243	ceramic nanoparticles (hydroxyapatite and bioglass)	223:273	The thought of biodegradable organic-inorganic composites composed of natural polymer chitosan and ceramic nanoparticles (hydroxyapatite and bioglass) can be considered as a solution for hard tissue engineering.
31923516	2	45	theme	chitosan-nanohydroxyapatite	392:418	arg1	scaffolds					467:475	chitosan-nanohydroxyapatite (CTS-nHA) and chitosan-nano-bioglass (CTS-nBG) scaffolds	392:475	chitosan-nanohydroxyapatite (CTS-nHA) and chitosan-nano-bioglass (CTS-nBG) scaffolds	392:475	In this paper, we described a comparative assessment of chitosan-nanohydroxyapatite (CTS-nHA) and chitosan-nano-bioglass (CTS-nBG) scaffolds.
31923516	6	46	theme	SEM	951:953	arg1	image					955:959	The SEM image	947:959	The SEM image	947:959	The SEM image confirmed the presence of spherically shaped nHA particles of 4.20 μm and irregularly shaped nBG particles of 6.89 μm.
31923516	12	47	theme	bioglass	1844:1851	arg1	nanoparticles					1853:1865	pre-loaded hydroxyapatite and bioglass nanoparticles	1814:1865	pre-loaded hydroxyapatite and bioglass nanoparticles	1814:1865	All the obtained results clearly recommend that pre-loaded hydroxyapatite and bioglass nanoparticles can enhance the apatite formation.
31923516	7	48	theme	nHA	1149:1151	arg1	particles					1153:1161	165.52 to 255.17 nm sized nHA particles	1123:1161	165.52 to 255.17 nm sized nHA particles	1123:1161	The TEM analysis revealed the existence of 165.52 to 255.17 nm sized nHA particles and 167.35 to 334.69 nm sized nBG particles.
31923516	3	49	from	dispersion	482:491	arg1	chitosan					550:557	chitosan	550:557	chitosan	550:557	The dispersion of nanoscaled hydroxyapatite (nHA) and bioglass (nBG) in chitosan remained satisfactory.
31923516	11	50	theme	fabricated	1596:1605	arg1	scaffolds					1607:1615	fabricated scaffolds	1596:1615	fabricated scaffolds	1596:1615	The cell culture experiment revealed that fabricated scaffolds had good compatibility with fibroblast cells (L929, ATCC) and MG-63 which are able to adhere, proliferate, and migrate through the porous structure.
31923516	1	51	theme	polymer	202:208	arg1	chitosan					210:217	natural polymer chitosan	194:217	natural polymer chitosan	194:217	The thought of biodegradable organic-inorganic composites composed of natural polymer chitosan and ceramic nanoparticles (hydroxyapatite and bioglass) can be considered as a solution for hard tissue engineering.
31923516	11	52	dep	cells	1656:1660	arg1	ATCC					1669:1672	ATCC	1669:1672	ATCC	1669:1672	The cell culture experiment revealed that fabricated scaffolds had good compatibility with fibroblast cells (L929, ATCC) and MG-63 which are able to adhere, proliferate, and migrate through the porous structure.
31923516	11	52	dep	cells	1656:1660	arg1	L929					1663:1666	L929	1663:1666	L929	1663:1666	The cell culture experiment revealed that fabricated scaffolds had good compatibility with fibroblast cells (L929, ATCC) and MG-63 which are able to adhere, proliferate, and migrate through the porous structure.
31923516	8	53	theme	CTS-nHA	1265:1271	arg1	structure					1252:1260	the interconnected structure	1233:1260	the interconnected structure of CTS-nHA and CTS-nBG nanocomposites	1233:1298	TEM analysis also showed the interconnected structure of CTS-nHA and CTS-nBG nanocomposites.
31923516	7	54	dep	255.17 nm	1133:1141	arg1	to					1130:1131	to	1130:1131	to	1130:1131	The TEM analysis revealed the existence of 165.52 to 255.17 nm sized nHA particles and 167.35 to 334.69 nm sized nBG particles.
31923516	10	55	theme	CTS-nHA	1442:1448	arg1	scaffolds					1450:1458	The CTS-nHA scaffolds	1438:1458	The CTS-nHA scaffolds	1438:1458	The CTS-nHA scaffolds exhibited enhanced compressive strength and elastic modulus compared with the CTS-nBG sample.
31923516	8	56	theme	TEM	1208:1210	arg1	analysis					1212:1219	TEM analysis	1208:1219	TEM analysis	1208:1219	TEM analysis also showed the interconnected structure of CTS-nHA and CTS-nBG nanocomposites.
31923516	6	57	theme	nHA	1006:1008	arg1	particles					1010:1018	spherically shaped nHA particles	987:1018	spherically shaped nHA particles of 4.20 μm	987:1029	The SEM image confirmed the presence of spherically shaped nHA particles of 4.20 μm and irregularly shaped nBG particles of 6.89 μm.
31923516	12	58	theme	obtained	1774:1781	arg1	results					1783:1789	All the obtained results	1766:1789	All the obtained results	1766:1789	All the obtained results clearly recommend that pre-loaded hydroxyapatite and bioglass nanoparticles can enhance the apatite formation.
31923516	8	59	theme	CTS-nBG	1277:1283	arg1	structure					1252:1260	the interconnected structure	1233:1260	the interconnected structure of CTS-nHA and CTS-nBG nanocomposites	1233:1298	TEM analysis also showed the interconnected structure of CTS-nHA and CTS-nBG nanocomposites.
31923516	5	60	theme	X-ray	757:761	arg1	XRD					783:785	XRD	783:785	XRD	783:785	The physiochemical and morphological analysis of all samples has been performed through X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
31923516	5	60	theme	X-ray	757:761	arg1	diffraction					770:780	X-ray powder diffraction	757:780	X-ray powder diffraction (XRD)	757:786	The physiochemical and morphological analysis of all samples has been performed through X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
31923516	5	61	theme	electron	876:883	arg1	SEM					897:899	SEM	897:899	SEM	897:899	The physiochemical and morphological analysis of all samples has been performed through X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
31923516	5	61	theme	electron	876:883	arg1	microscopy					885:894	scanning electron microscopy	867:894	scanning electron microscopy (SEM)	867:900	The physiochemical and morphological analysis of all samples has been performed through X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
31923516	1	62	theme	natural	194:200	arg1	chitosan					210:217	natural polymer chitosan	194:217	natural polymer chitosan	194:217	The thought of biodegradable organic-inorganic composites composed of natural polymer chitosan and ceramic nanoparticles (hydroxyapatite and bioglass) can be considered as a solution for hard tissue engineering.
31923516	7	63	theme	TEM	1084:1086	arg1	analysis					1088:1095	The TEM analysis	1080:1095	The TEM analysis	1080:1095	The TEM analysis revealed the existence of 165.52 to 255.17 nm sized nHA particles and 167.35 to 334.69 nm sized nBG particles.
31923516	5	64	theme	transmission	907:918	arg1	TEM					941:943	TEM	941:943	TEM	941:943	The physiochemical and morphological analysis of all samples has been performed through X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
31923516	5	64	theme	transmission	907:918	arg1	microscopy					929:938	transmission electron microscopy	907:938	transmission electron microscopy (TEM)	907:944	The physiochemical and morphological analysis of all samples has been performed through X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
31923516	1	65	theme	biodegradable	139:151	arg1	composites					171:180	biodegradable organic-inorganic composites	139:180	biodegradable organic-inorganic composites composed of natural polymer chitosan and ceramic nanoparticles (hydroxyapatite and bioglass)	139:273	The thought of biodegradable organic-inorganic composites composed of natural polymer chitosan and ceramic nanoparticles (hydroxyapatite and bioglass) can be considered as a solution for hard tissue engineering.
31923516	11	66	contain	had	1617:1619	arg2	compatibility					1626:1638	good compatibility	1621:1638	good compatibility	1621:1638	The cell culture experiment revealed that fabricated scaffolds had good compatibility with fibroblast cells (L929, ATCC) and MG-63 which are able to adhere, proliferate, and migrate through the porous structure.
31923516	11	66	contain	had	1617:1619	arg1	scaffolds					1607:1615	fabricated scaffolds	1596:1615	fabricated scaffolds	1596:1615	The cell culture experiment revealed that fabricated scaffolds had good compatibility with fibroblast cells (L929, ATCC) and MG-63 which are able to adhere, proliferate, and migrate through the porous structure.
31923516	13	67	with	scaffolds	1906:1914	arg1	bioglass					1931:1938	bioglass	1931:1938	bioglass	1931:1938	The scaffolds with chitosan, bioglass, and hydroxyapatite have better biomechanical characteristics and allow cell growth.
31923516	13	67	with	scaffolds	1906:1914	arg1	hydroxyapatite					1945:1958	hydroxyapatite	1945:1958	hydroxyapatite	1945:1958	The scaffolds with chitosan, bioglass, and hydroxyapatite have better biomechanical characteristics and allow cell growth.
31923516	13	67	with	scaffolds	1906:1914	arg1	chitosan					1921:1928	chitosan	1921:1928	chitosan	1921:1928	The scaffolds with chitosan, bioglass, and hydroxyapatite have better biomechanical characteristics and allow cell growth.
31923516	13	68	theme	biomechanical	1972:1984	arg1	characteristics					1986:2000	better biomechanical characteristics	1965:2000	better biomechanical characteristics	1965:2000	The scaffolds with chitosan, bioglass, and hydroxyapatite have better biomechanical characteristics and allow cell growth.
31923516	5	69	theme	infrared	807:814	arg1	spectroscopy					816:827	Fourier-transform infrared spectroscopy	789:827	Fourier-transform infrared spectroscopy (FTIR)	789:834	The physiochemical and morphological analysis of all samples has been performed through X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
31923516	5	69	theme	infrared	807:814	arg1	FTIR					830:833	FTIR	830:833	FTIR	830:833	The physiochemical and morphological analysis of all samples has been performed through X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
31923516	5	70	theme	electron	920:927	arg1	TEM					941:943	TEM	941:943	TEM	941:943	The physiochemical and morphological analysis of all samples has been performed through X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
31923516	5	70	theme	electron	920:927	arg1	microscopy					929:938	transmission electron microscopy	907:938	transmission electron microscopy (TEM)	907:944	The physiochemical and morphological analysis of all samples has been performed through X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
31923516	6	71	theme	shaped	1047:1052	arg1	particles					1058:1066	irregularly shaped nBG particles	1035:1066	irregularly shaped nBG particles of 6.89 μm	1035:1077	The SEM image confirmed the presence of spherically shaped nHA particles of 4.20 μm and irregularly shaped nBG particles of 6.89 μm.
31923516	11	72	theme	cell	1558:1561	arg1	experiment					1571:1580	The cell culture experiment	1554:1580	The cell culture experiment	1554:1580	The cell culture experiment revealed that fabricated scaffolds had good compatibility with fibroblast cells (L929, ATCC) and MG-63 which are able to adhere, proliferate, and migrate through the porous structure.
31923516	0	73	theme	bone	92:95	arg1	applications					110:121	bone regeneration applications	92:121	bone regeneration applications	92:121	Fabrication and in-vitro biocompatibility of freeze-dried CTS-nHA and CTS-nBG scaffolds for bone regeneration applications.
31923516	9	74	theme	incubation	1319:1328	arg1	period					1330:1335	seven days' incubation period	1307:1335	seven days' incubation period	1307:1335	After seven days' incubation period, the CTS-nHA and CTS-nBG scaffolds shown good mineralization behavior in simulated body fluid (SBF).
31923516	10	75	theme	compressive	1479:1489	arg1	strength					1491:1498	enhanced compressive strength	1470:1498	enhanced compressive strength	1470:1498	The CTS-nHA scaffolds exhibited enhanced compressive strength and elastic modulus compared with the CTS-nBG sample.
31923516	9	76	from	behavior	1398:1405	arg1	SBF					1432:1434	SBF	1432:1434	SBF	1432:1434	After seven days' incubation period, the CTS-nHA and CTS-nBG scaffolds shown good mineralization behavior in simulated body fluid (SBF).
31923516	9	76	from	behavior	1398:1405	arg1	fluid					1425:1429	simulated body fluid	1410:1429	simulated body fluid (SBF)	1410:1435	After seven days' incubation period, the CTS-nHA and CTS-nBG scaffolds shown good mineralization behavior in simulated body fluid (SBF).
31923516	14	77	theme	hard	2090:2093	arg1	applications					2114:2125	various hard tissue engineering applications	2082:2125	various hard tissue engineering applications such as bone regeneration	2082:2151	Therefore, these scaffolds can be perfect candidates for various hard tissue engineering applications such as bone regeneration.
31923516	14	77	theme	hard	2090:2093	arg1	regeneration					2140:2151	bone regeneration	2135:2151	bone regeneration	2135:2151	Therefore, these scaffolds can be perfect candidates for various hard tissue engineering applications such as bone regeneration.
31923516	9	78	theme	CTS-nHA	1342:1348	arg1	scaffolds					1362:1370	the CTS-nHA and CTS-nBG scaffolds	1338:1370	the CTS-nHA and CTS-nBG scaffolds	1338:1370	After seven days' incubation period, the CTS-nHA and CTS-nBG scaffolds shown good mineralization behavior in simulated body fluid (SBF).
31923516	7	79	theme	particles	1197:1205	arg1	existence					1110:1118	the existence	1106:1118	the existence of 165.52 to 255.17 nm sized nHA particles and 167.35 to 334.69 nm sized nBG particles	1106:1205	The TEM analysis revealed the existence of 165.52 to 255.17 nm sized nHA particles and 167.35 to 334.69 nm sized nBG particles.
31923516	9	80	theme	CTS-nBG	1354:1360	arg1	scaffolds					1362:1370	the CTS-nHA and CTS-nBG scaffolds	1338:1370	the CTS-nHA and CTS-nBG scaffolds	1338:1370	After seven days' incubation period, the CTS-nHA and CTS-nBG scaffolds shown good mineralization behavior in simulated body fluid (SBF).
31923516	14	81	theme	perfect	2059:2065	arg1	candidates					2067:2076	perfect candidates	2059:2076	perfect candidates for various hard tissue engineering applications such as bone regeneration	2059:2151	Therefore, these scaffolds can be perfect candidates for various hard tissue engineering applications such as bone regeneration.
31923516	4	82	theme	freeze-dried	586:597	arg1	CTS-nHA					615:621	The freeze-dried composite based CTS-nHA	582:621	The freeze-dried composite based CTS-nHA	582:621	The freeze-dried composite based CTS-nHA and CTS-nBG scaffolds shown porous structure.
31923516	7	83	dep	334.69 nm	1177:1185	arg1	to					1174:1175	to	1174:1175	to	1174:1175	The TEM analysis revealed the existence of 165.52 to 255.17 nm sized nHA particles and 167.35 to 334.69 nm sized nBG particles.
31923516	7	84	theme	sized	1187:1191	arg1	particles					1197:1205	167.35 to 334.69 nm sized nBG particles	1167:1205	167.35 to 334.69 nm sized nBG particles	1167:1205	The TEM analysis revealed the existence of 165.52 to 255.17 nm sized nHA particles and 167.35 to 334.69 nm sized nBG particles.
31923516	1	85	theme	hard	311:314	arg1	engineering					323:333	hard tissue engineering	311:333	hard tissue engineering	311:333	The thought of biodegradable organic-inorganic composites composed of natural polymer chitosan and ceramic nanoparticles (hydroxyapatite and bioglass) can be considered as a solution for hard tissue engineering.
31923516	2	86	theme	scaffolds	467:475	arg1	assessment					378:387	a comparative assessment	364:387	a comparative assessment of chitosan-nanohydroxyapatite (CTS-nHA) and chitosan-nano-bioglass (CTS-nBG) scaffolds	364:475	In this paper, we described a comparative assessment of chitosan-nanohydroxyapatite (CTS-nHA) and chitosan-nano-bioglass (CTS-nBG) scaffolds.
31923516	4	87	theme	based	609:613	arg1	CTS-nHA					615:621	The freeze-dried composite based CTS-nHA	582:621	The freeze-dried composite based CTS-nHA	582:621	The freeze-dried composite based CTS-nHA and CTS-nBG scaffolds shown porous structure.
31923516	10	88	theme	CTS-nBG	1538:1544	arg1	sample					1546:1551	the CTS-nBG sample	1534:1551	the CTS-nBG sample	1534:1551	The CTS-nHA scaffolds exhibited enhanced compressive strength and elastic modulus compared with the CTS-nBG sample.
31923516	0	89	theme	freeze-dried	45:56	arg1	scaffolds					78:86	freeze-dried CTS-nHA and CTS-nBG scaffolds	45:86	freeze-dried CTS-nHA and CTS-nBG scaffolds	45:86	Fabrication and in-vitro biocompatibility of freeze-dried CTS-nHA and CTS-nBG scaffolds for bone regeneration applications.
31923516	9	90	theme	mineralization	1383:1396	arg1	behavior					1398:1405	good mineralization behavior	1378:1405	good mineralization behavior in simulated body fluid (SBF)	1378:1435	After seven days' incubation period, the CTS-nHA and CTS-nBG scaffolds shown good mineralization behavior in simulated body fluid (SBF).
31923516	6	91	theme	6.89 μm	1071:1077	arg1	particles					1010:1018	spherically shaped nHA particles	987:1018	spherically shaped nHA particles of 4.20 μm	987:1029	The SEM image confirmed the presence of spherically shaped nHA particles of 4.20 μm and irregularly shaped nBG particles of 6.89 μm.
31923516	6	91	theme	6.89 μm	1071:1077	arg1	particles					1058:1066	irregularly shaped nBG particles	1035:1066	irregularly shaped nBG particles of 6.89 μm	1035:1077	The SEM image confirmed the presence of spherically shaped nHA particles of 4.20 μm and irregularly shaped nBG particles of 6.89 μm.
31923516	2	92	theme	chitosan-nano-bioglass	434:455	arg1	scaffolds					467:475	chitosan-nanohydroxyapatite (CTS-nHA) and chitosan-nano-bioglass (CTS-nBG) scaffolds	392:475	chitosan-nanohydroxyapatite (CTS-nHA) and chitosan-nano-bioglass (CTS-nBG) scaffolds	392:475	In this paper, we described a comparative assessment of chitosan-nanohydroxyapatite (CTS-nHA) and chitosan-nano-bioglass (CTS-nBG) scaffolds.
31923516	11	93	theme	porous	1748:1753	arg1	structure					1755:1763	the porous structure	1744:1763	the porous structure	1744:1763	The cell culture experiment revealed that fabricated scaffolds had good compatibility with fibroblast cells (L929, ATCC) and MG-63 which are able to adhere, proliferate, and migrate through the porous structure.
31923516	3	94	theme	bioglass	532:539	arg1	dispersion					482:491	The dispersion	478:491	The dispersion of nanoscaled hydroxyapatite (nHA) and bioglass (nBG) in chitosan	478:557	The dispersion of nanoscaled hydroxyapatite (nHA) and bioglass (nBG) in chitosan remained satisfactory.
31923516	9	95	theme	body	1420:1423	arg1	SBF					1432:1434	SBF	1432:1434	SBF	1432:1434	After seven days' incubation period, the CTS-nHA and CTS-nBG scaffolds shown good mineralization behavior in simulated body fluid (SBF).
31923516	9	95	theme	body	1420:1423	arg1	fluid					1425:1429	simulated body fluid	1410:1429	simulated body fluid (SBF)	1410:1435	After seven days' incubation period, the CTS-nHA and CTS-nBG scaffolds shown good mineralization behavior in simulated body fluid (SBF).
31923516	4	96	theme	porous	651:656	arg1	structure					658:666	porous structure	651:666	porous structure	651:666	The freeze-dried composite based CTS-nHA and CTS-nBG scaffolds shown porous structure.
31923516	14	97	theme	tissue	2095:2100	arg1	applications					2114:2125	various hard tissue engineering applications	2082:2125	various hard tissue engineering applications such as bone regeneration	2082:2151	Therefore, these scaffolds can be perfect candidates for various hard tissue engineering applications such as bone regeneration.
31923516	14	97	theme	tissue	2095:2100	arg1	regeneration					2140:2151	bone regeneration	2135:2151	bone regeneration	2135:2151	Therefore, these scaffolds can be perfect candidates for various hard tissue engineering applications such as bone regeneration.
31923516	12	98	theme	pre-loaded	1814:1823	arg1	nanoparticles					1853:1865	pre-loaded hydroxyapatite and bioglass nanoparticles	1814:1865	pre-loaded hydroxyapatite and bioglass nanoparticles	1814:1865	All the obtained results clearly recommend that pre-loaded hydroxyapatite and bioglass nanoparticles can enhance the apatite formation.
32567254	7	0	from	dressing	1536:1543	arg1	wounds					1556:1561	chronic wounds care	1548:1566	chronic wounds care	1548:1566	Compared with other antimicrobial wound dressing composite materials, the proposed BC/MGO nanocomposite has among the greatest antimicrobial property against broad-spectrum bacteria, making it a promising antimicrobial dressing in chronic wounds care.
32567254	3	1	theme	BC/MGO	705:710	arg1	composite					712:720	the BC/MGO composite	701:720	the BC/MGO composite	701:720	Characterizations by scanning electron microscope and atomic force microscopy show the interconnected nanostructure of BC and MGO and increase surface roughness of the BC/MGO composite.
32567254	7	2	theme	broad-spectrum	1475:1488	arg1	bacteria					1490:1497	broad-spectrum bacteria	1475:1497	broad-spectrum bacteria	1475:1497	Compared with other antimicrobial wound dressing composite materials, the proposed BC/MGO nanocomposite has among the greatest antimicrobial property against broad-spectrum bacteria, making it a promising antimicrobial dressing in chronic wounds care.
32567254	2	3	theme	BC/methylglyoxal	359:374	arg1	composite					382:390	BC/methylglyoxal (MGO) composite	359:390	BC/methylglyoxal (MGO) composite	359:390	In this paper, a novel antimicrobial dressing made from BC/methylglyoxal (MGO) composite with a dip-coating method inspired by naturally antimicrobial Manuka honey is proposed, which to our best knowledge, has not yet to be reported.
32567254	4	4	theme	BC	785:786	arg1	stability					767:775	high temperature stability	750:775	high temperature stability of both BC and BC/MGO	750:797	Thermal analysis indicates high temperature stability of both BC and BC/MGO, while compared with BC, BC/MGO exhibits slightly weaker thermal stability possibly due to reduction of hydrogen bonding and increase of crystallinity.
32567254	1	5	theme	water-holding	249:261	arg1	capacity					263:270	high water-holding capacity	244:270	high water-holding capacity	244:270	Bacterial cellulose (BC) is a natural material produced by Acetobacter xylinum, widely used in wound dressings due to the high water-holding capacity and great mechanical strength.
32567254	0	6	theme	Wound	106:110	arg1	Dressing					112:119	Broad-Spectrum Antimicrobial Wound Dressing	77:119	Broad-Spectrum Antimicrobial Wound Dressing	77:119	Nature-Inspired Bacterial Cellulose/Methylglyoxal (BC/MGO) Nanocomposite for Broad-Spectrum Antimicrobial Wound Dressing.
32567254	7	7	theme	promising	1512:1520	arg1	dressing					1536:1543	a promising antimicrobial dressing	1510:1543	a promising antimicrobial dressing in chronic wounds care	1510:1566	Compared with other antimicrobial wound dressing composite materials, the proposed BC/MGO nanocomposite has among the greatest antimicrobial property against broad-spectrum bacteria, making it a promising antimicrobial dressing in chronic wounds care.
32567254	6	8	theme	MGO	1121:1123	arg1	concentration					1125:1137	highest MGO concentration	1113:1137	highest MGO concentration (4%)	1113:1142	From the disk diffusion antimicrobial test, the BC/MGO nanocomposite with highest MGO concentration (4%) shows great zone inhibition diameter (around 14.3, 12.3, 17.1, and 15.5 mm against Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli).
32567254	6	8	theme	MGO	1121:1123	arg1	%					1141:1141	4%	1140:1141	4%	1140:1141	From the disk diffusion antimicrobial test, the BC/MGO nanocomposite with highest MGO concentration (4%) shows great zone inhibition diameter (around 14.3, 12.3, 17.1, and 15.5 mm against Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli).
32567254	6	9	theme	highest	1113:1119	arg1	concentration					1125:1137	highest MGO concentration	1113:1137	highest MGO concentration (4%)	1113:1142	From the disk diffusion antimicrobial test, the BC/MGO nanocomposite with highest MGO concentration (4%) shows great zone inhibition diameter (around 14.3, 12.3, 17.1, and 15.5 mm against Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli).
32567254	6	9	theme	highest	1113:1119	arg1	%					1141:1141	4%	1140:1141	4%	1140:1141	From the disk diffusion antimicrobial test, the BC/MGO nanocomposite with highest MGO concentration (4%) shows great zone inhibition diameter (around 14.3, 12.3, 17.1, and 15.5 mm against Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli).
32567254	4	10	theme	BC/MGO	792:797	arg1	stability					767:775	high temperature stability	750:775	high temperature stability of both BC and BC/MGO	750:797	Thermal analysis indicates high temperature stability of both BC and BC/MGO, while compared with BC, BC/MGO exhibits slightly weaker thermal stability possibly due to reduction of hydrogen bonding and increase of crystallinity.
32567254	7	11	theme	greatest	1435:1442	arg1	property					1458:1465	the greatest antimicrobial property	1431:1465	the greatest antimicrobial property against broad-spectrum bacteria	1431:1497	Compared with other antimicrobial wound dressing composite materials, the proposed BC/MGO nanocomposite has among the greatest antimicrobial property against broad-spectrum bacteria, making it a promising antimicrobial dressing in chronic wounds care.
32567254	5	12	theme	BC	1010:1011	arg1	property					998:1005	the strong mechanical property	976:1005	the strong mechanical property of BC and BC/MGO nanocomposite	976:1036	Mechanical test confirms the strong mechanical property of BC and BC/MGO nanocomposite.
32567254	4	13	theme	high	750:753	arg1	stability					767:775	high temperature stability	750:775	high temperature stability of both BC and BC/MGO	750:797	Thermal analysis indicates high temperature stability of both BC and BC/MGO, while compared with BC, BC/MGO exhibits slightly weaker thermal stability possibly due to reduction of hydrogen bonding and increase of crystallinity.
32567254	6	14	theme	zone	1156:1159	arg1	diameter					1172:1179	great zone inhibition diameter	1150:1179	great zone inhibition diameter (around 14.3, 12.3, 17.1, and 15.5 mm against Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli)	1150:1314	From the disk diffusion antimicrobial test, the BC/MGO nanocomposite with highest MGO concentration (4%) shows great zone inhibition diameter (around 14.3, 12.3, 17.1, and 15.5 mm against Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli).
32567254	7	15	theme	antimicrobial	1444:1456	arg1	property					1458:1465	the greatest antimicrobial property	1431:1465	the greatest antimicrobial property against broad-spectrum bacteria	1431:1497	Compared with other antimicrobial wound dressing composite materials, the proposed BC/MGO nanocomposite has among the greatest antimicrobial property against broad-spectrum bacteria, making it a promising antimicrobial dressing in chronic wounds care.
32567254	5	16	theme	strong	980:985	arg1	property					998:1005	the strong mechanical property	976:1005	the strong mechanical property of BC and BC/MGO nanocomposite	976:1036	Mechanical test confirms the strong mechanical property of BC and BC/MGO nanocomposite.
32567254	1	17	theme	great	276:280	arg1	strength					293:300	great mechanical strength	276:300	great mechanical strength	276:300	Bacterial cellulose (BC) is a natural material produced by Acetobacter xylinum, widely used in wound dressings due to the high water-holding capacity and great mechanical strength.
32567254	6	18	theme	antimicrobial	1063:1075	arg1	test					1077:1080	the disk diffusion antimicrobial test	1044:1080	the disk diffusion antimicrobial test	1044:1080	From the disk diffusion antimicrobial test, the BC/MGO nanocomposite with highest MGO concentration (4%) shows great zone inhibition diameter (around 14.3, 12.3, 17.1, and 15.5 mm against Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli).
32567254	5	19	theme	mechanical	987:996	arg1	property					998:1005	the strong mechanical property	976:1005	the strong mechanical property of BC and BC/MGO nanocomposite	976:1036	Mechanical test confirms the strong mechanical property of BC and BC/MGO nanocomposite.
32567254	1	20	theme	Acetobacter	181:191	arg1	xylinum					193:199	Acetobacter xylinum	181:199	Acetobacter xylinum	181:199	Bacterial cellulose (BC) is a natural material produced by Acetobacter xylinum, widely used in wound dressings due to the high water-holding capacity and great mechanical strength.
32567254	3	21	theme	composite	712:720	arg1	roughness					688:696	surface roughness	680:696	surface roughness of the BC/MGO composite	680:720	Characterizations by scanning electron microscope and atomic force microscopy show the interconnected nanostructure of BC and MGO and increase surface roughness of the BC/MGO composite.
32567254	0	22	theme	Nature-Inspired	0:14	arg1	Nanocomposite					59:71	Nature-Inspired Bacterial Cellulose/Methylglyoxal (BC/MGO) Nanocomposite	0:71	Nature-Inspired Bacterial Cellulose/Methylglyoxal (BC/MGO) Nanocomposite for Broad-Spectrum Antimicrobial Wound Dressing.	0:120	Nature-Inspired Bacterial Cellulose/Methylglyoxal (BC/MGO) Nanocomposite for Broad-Spectrum Antimicrobial Wound Dressing.
32567254	6	23	theme	BC/MGO	1087:1092	arg1	nanocomposite					1094:1106	the BC/MGO nanocomposite	1083:1106	the BC/MGO nanocomposite with highest MGO concentration (4%)	1083:1142	From the disk diffusion antimicrobial test, the BC/MGO nanocomposite with highest MGO concentration (4%) shows great zone inhibition diameter (around 14.3, 12.3, 17.1, and 15.5 mm against Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli).
32567254	0	24	theme	Bacterial	16:24	arg1	Nanocomposite					59:71	Nature-Inspired Bacterial Cellulose/Methylglyoxal (BC/MGO) Nanocomposite	0:71	Nature-Inspired Bacterial Cellulose/Methylglyoxal (BC/MGO) Nanocomposite for Broad-Spectrum Antimicrobial Wound Dressing.	0:120	Nature-Inspired Bacterial Cellulose/Methylglyoxal (BC/MGO) Nanocomposite for Broad-Spectrum Antimicrobial Wound Dressing.
32567254	6	25	theme	inhibition	1161:1170	arg1	diameter					1172:1179	great zone inhibition diameter	1150:1179	great zone inhibition diameter (around 14.3, 12.3, 17.1, and 15.5 mm against Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli)	1150:1314	From the disk diffusion antimicrobial test, the BC/MGO nanocomposite with highest MGO concentration (4%) shows great zone inhibition diameter (around 14.3, 12.3, 17.1, and 15.5 mm against Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli).
32567254	4	26	theme	thermal	856:862	arg1	stability					864:872	slightly weaker thermal stability	840:872	slightly weaker thermal stability	840:872	Thermal analysis indicates high temperature stability of both BC and BC/MGO, while compared with BC, BC/MGO exhibits slightly weaker thermal stability possibly due to reduction of hydrogen bonding and increase of crystallinity.
32567254	3	27	theme	scanning	558:565	arg1	microscope					576:585	scanning electron microscope	558:585	scanning electron microscope	558:585	Characterizations by scanning electron microscope and atomic force microscopy show the interconnected nanostructure of BC and MGO and increase surface roughness of the BC/MGO composite.
32567254	2	28	theme	Manuka	454:459	arg1	honey					461:465	naturally antimicrobial Manuka honey	430:465	naturally antimicrobial Manuka honey	430:465	In this paper, a novel antimicrobial dressing made from BC/methylglyoxal (MGO) composite with a dip-coating method inspired by naturally antimicrobial Manuka honey is proposed, which to our best knowledge, has not yet to be reported.
32567254	1	29	theme	mechanical	282:291	arg1	strength					293:300	great mechanical strength	276:300	great mechanical strength	276:300	Bacterial cellulose (BC) is a natural material produced by Acetobacter xylinum, widely used in wound dressings due to the high water-holding capacity and great mechanical strength.
32567254	4	30	theme	weaker	849:854	arg1	stability					864:872	slightly weaker thermal stability	840:872	slightly weaker thermal stability	840:872	Thermal analysis indicates high temperature stability of both BC and BC/MGO, while compared with BC, BC/MGO exhibits slightly weaker thermal stability possibly due to reduction of hydrogen bonding and increase of crystallinity.
32567254	1	31	theme	Bacterial	122:130	arg1	material					160:167	a natural material	150:167	a natural material	150:167	Bacterial cellulose (BC) is a natural material produced by Acetobacter xylinum, widely used in wound dressings due to the high water-holding capacity and great mechanical strength.
32567254	1	31	theme	Bacterial	122:130	arg1	BC					143:144	BC	143:144	BC	143:144	Bacterial cellulose (BC) is a natural material produced by Acetobacter xylinum, widely used in wound dressings due to the high water-holding capacity and great mechanical strength.
32567254	1	31	theme	Bacterial	122:130	arg1	cellulose					132:140	Bacterial cellulose	122:140	Bacterial cellulose (BC)	122:145	Bacterial cellulose (BC) is a natural material produced by Acetobacter xylinum, widely used in wound dressings due to the high water-holding capacity and great mechanical strength.
32567254	7	32	theme	dressing	1357:1364	arg1	materials					1376:1384	other antimicrobial wound dressing composite materials	1331:1384	other antimicrobial wound dressing composite materials	1331:1384	Compared with other antimicrobial wound dressing composite materials, the proposed BC/MGO nanocomposite has among the greatest antimicrobial property against broad-spectrum bacteria, making it a promising antimicrobial dressing in chronic wounds care.
32567254	2	33	theme	antimicrobial	440:452	arg1	honey					461:465	naturally antimicrobial Manuka honey	430:465	naturally antimicrobial Manuka honey	430:465	In this paper, a novel antimicrobial dressing made from BC/methylglyoxal (MGO) composite with a dip-coating method inspired by naturally antimicrobial Manuka honey is proposed, which to our best knowledge, has not yet to be reported.
32567254	6	34	theme	great	1150:1154	arg1	diameter					1172:1179	great zone inhibition diameter	1150:1179	great zone inhibition diameter (around 14.3, 12.3, 17.1, and 15.5 mm against Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli)	1150:1314	From the disk diffusion antimicrobial test, the BC/MGO nanocomposite with highest MGO concentration (4%) shows great zone inhibition diameter (around 14.3, 12.3, 17.1, and 15.5 mm against Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli).
32567254	0	35	theme	Cellulose/Methylglyoxal	26:48	arg1	Nanocomposite					59:71	Nature-Inspired Bacterial Cellulose/Methylglyoxal (BC/MGO) Nanocomposite	0:71	Nature-Inspired Bacterial Cellulose/Methylglyoxal (BC/MGO) Nanocomposite for Broad-Spectrum Antimicrobial Wound Dressing.	0:120	Nature-Inspired Bacterial Cellulose/Methylglyoxal (BC/MGO) Nanocomposite for Broad-Spectrum Antimicrobial Wound Dressing.
32567254	5	36	theme	Mechanical	951:960	arg1	test					962:965	Mechanical test	951:965	Mechanical test	951:965	Mechanical test confirms the strong mechanical property of BC and BC/MGO nanocomposite.
32567254	3	37	theme	BC	656:657	arg1	nanostructure					639:651	the interconnected nanostructure	620:651	the interconnected nanostructure of BC and MGO	620:665	Characterizations by scanning electron microscope and atomic force microscopy show the interconnected nanostructure of BC and MGO and increase surface roughness of the BC/MGO composite.
32567254	4	38	theme	temperature	755:765	arg1	stability					767:775	high temperature stability	750:775	high temperature stability of both BC and BC/MGO	750:797	Thermal analysis indicates high temperature stability of both BC and BC/MGO, while compared with BC, BC/MGO exhibits slightly weaker thermal stability possibly due to reduction of hydrogen bonding and increase of crystallinity.
32567254	1	39	theme	natural	152:158	arg1	material					160:167	a natural material	150:167	a natural material	150:167	Bacterial cellulose (BC) is a natural material produced by Acetobacter xylinum, widely used in wound dressings due to the high water-holding capacity and great mechanical strength.
32567254	1	39	theme	natural	152:158	arg1	cellulose					132:140	Bacterial cellulose	122:140	Bacterial cellulose (BC)	122:145	Bacterial cellulose (BC) is a natural material produced by Acetobacter xylinum, widely used in wound dressings due to the high water-holding capacity and great mechanical strength.
32567254	4	40	theme	crystallinity	936:948	arg1	increase					924:931	increase	924:931	increase of crystallinity	924:948	Thermal analysis indicates high temperature stability of both BC and BC/MGO, while compared with BC, BC/MGO exhibits slightly weaker thermal stability possibly due to reduction of hydrogen bonding and increase of crystallinity.
32567254	4	40	theme	crystallinity	936:948	arg1	reduction					890:898	reduction	890:898	reduction of hydrogen bonding	890:918	Thermal analysis indicates high temperature stability of both BC and BC/MGO, while compared with BC, BC/MGO exhibits slightly weaker thermal stability possibly due to reduction of hydrogen bonding and increase of crystallinity.
32567254	6	41	theme	diffusion	1053:1061	arg1	test					1077:1080	the disk diffusion antimicrobial test	1044:1080	the disk diffusion antimicrobial test	1044:1080	From the disk diffusion antimicrobial test, the BC/MGO nanocomposite with highest MGO concentration (4%) shows great zone inhibition diameter (around 14.3, 12.3, 17.1, and 15.5 mm against Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli).
32567254	3	42	theme	atomic	591:596	arg1	microscopy					604:613	atomic force microscopy	591:613	atomic force microscopy	591:613	Characterizations by scanning electron microscope and atomic force microscopy show the interconnected nanostructure of BC and MGO and increase surface roughness of the BC/MGO composite.
32567254	1	43	theme	wound	217:221	arg1	dressings					223:231	wound dressings	217:231	wound dressings due to the high water-holding capacity and great mechanical strength	217:300	Bacterial cellulose (BC) is a natural material produced by Acetobacter xylinum, widely used in wound dressings due to the high water-holding capacity and great mechanical strength.
32567254	0	44	theme	BC/MGO	51:56	arg1	Nanocomposite					59:71	Nature-Inspired Bacterial Cellulose/Methylglyoxal (BC/MGO) Nanocomposite	0:71	Nature-Inspired Bacterial Cellulose/Methylglyoxal (BC/MGO) Nanocomposite for Broad-Spectrum Antimicrobial Wound Dressing.	0:120	Nature-Inspired Bacterial Cellulose/Methylglyoxal (BC/MGO) Nanocomposite for Broad-Spectrum Antimicrobial Wound Dressing.
32567254	6	45	with	nanocomposite	1094:1106	arg1	concentration					1125:1137	highest MGO concentration	1113:1137	highest MGO concentration (4%)	1113:1142	From the disk diffusion antimicrobial test, the BC/MGO nanocomposite with highest MGO concentration (4%) shows great zone inhibition diameter (around 14.3, 12.3, 17.1, and 15.5 mm against Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli).
32567254	6	45	with	nanocomposite	1094:1106	arg1	%					1141:1141	4%	1140:1141	4%	1140:1141	From the disk diffusion antimicrobial test, the BC/MGO nanocomposite with highest MGO concentration (4%) shows great zone inhibition diameter (around 14.3, 12.3, 17.1, and 15.5 mm against Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli).
32567254	3	46	theme	force	598:602	arg1	microscopy					604:613	atomic force microscopy	591:613	atomic force microscopy	591:613	Characterizations by scanning electron microscope and atomic force microscopy show the interconnected nanostructure of BC and MGO and increase surface roughness of the BC/MGO composite.
32567254	2	47	theme	dip-coating	399:409	arg1	method					411:416	a dip-coating method	397:416	a dip-coating method inspired by naturally antimicrobial Manuka honey	397:465	In this paper, a novel antimicrobial dressing made from BC/methylglyoxal (MGO) composite with a dip-coating method inspired by naturally antimicrobial Manuka honey is proposed, which to our best knowledge, has not yet to be reported.
32567254	7	48	theme	antimicrobial	1522:1534	arg1	dressing					1536:1543	a promising antimicrobial dressing	1510:1543	a promising antimicrobial dressing in chronic wounds care	1510:1566	Compared with other antimicrobial wound dressing composite materials, the proposed BC/MGO nanocomposite has among the greatest antimicrobial property against broad-spectrum bacteria, making it a promising antimicrobial dressing in chronic wounds care.
32567254	6	49	theme	disk	1048:1051	arg1	test					1077:1080	the disk diffusion antimicrobial test	1044:1080	the disk diffusion antimicrobial test	1044:1080	From the disk diffusion antimicrobial test, the BC/MGO nanocomposite with highest MGO concentration (4%) shows great zone inhibition diameter (around 14.3, 12.3, 17.1, and 15.5 mm against Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli).
32567254	0	50	theme	Broad-Spectrum	77:90	arg1	Dressing					112:119	Broad-Spectrum Antimicrobial Wound Dressing	77:119	Broad-Spectrum Antimicrobial Wound Dressing	77:119	Nature-Inspired Bacterial Cellulose/Methylglyoxal (BC/MGO) Nanocomposite for Broad-Spectrum Antimicrobial Wound Dressing.
32567254	7	51	theme	composite	1366:1374	arg1	materials					1376:1384	other antimicrobial wound dressing composite materials	1331:1384	other antimicrobial wound dressing composite materials	1331:1384	Compared with other antimicrobial wound dressing composite materials, the proposed BC/MGO nanocomposite has among the greatest antimicrobial property against broad-spectrum bacteria, making it a promising antimicrobial dressing in chronic wounds care.
32567254	7	52	theme	BC/MGO	1400:1405	arg1	nanocomposite					1407:1419	the proposed BC/MGO nanocomposite	1387:1419	the proposed BC/MGO nanocomposite	1387:1419	Compared with other antimicrobial wound dressing composite materials, the proposed BC/MGO nanocomposite has among the greatest antimicrobial property against broad-spectrum bacteria, making it a promising antimicrobial dressing in chronic wounds care.
32567254	7	53	theme	chronic	1548:1554	arg1	wounds					1556:1561	chronic wounds care	1548:1566	chronic wounds care	1548:1566	Compared with other antimicrobial wound dressing composite materials, the proposed BC/MGO nanocomposite has among the greatest antimicrobial property against broad-spectrum bacteria, making it a promising antimicrobial dressing in chronic wounds care.
32567254	7	54	theme	antimicrobial	1337:1349	arg1	materials					1376:1384	other antimicrobial wound dressing composite materials	1331:1384	other antimicrobial wound dressing composite materials	1331:1384	Compared with other antimicrobial wound dressing composite materials, the proposed BC/MGO nanocomposite has among the greatest antimicrobial property against broad-spectrum bacteria, making it a promising antimicrobial dressing in chronic wounds care.
32567254	4	55	theme	bonding	912:918	arg1	increase					924:931	increase	924:931	increase of crystallinity	924:948	Thermal analysis indicates high temperature stability of both BC and BC/MGO, while compared with BC, BC/MGO exhibits slightly weaker thermal stability possibly due to reduction of hydrogen bonding and increase of crystallinity.
32567254	4	55	theme	bonding	912:918	arg1	reduction					890:898	reduction	890:898	reduction of hydrogen bonding	890:918	Thermal analysis indicates high temperature stability of both BC and BC/MGO, while compared with BC, BC/MGO exhibits slightly weaker thermal stability possibly due to reduction of hydrogen bonding and increase of crystallinity.
32567254	3	56	theme	surface	680:686	arg1	roughness					688:696	surface roughness	680:696	surface roughness of the BC/MGO composite	680:720	Characterizations by scanning electron microscope and atomic force microscopy show the interconnected nanostructure of BC and MGO and increase surface roughness of the BC/MGO composite.
32567254	7	57	theme	wound	1351:1355	arg1	materials					1376:1384	other antimicrobial wound dressing composite materials	1331:1384	other antimicrobial wound dressing composite materials	1331:1384	Compared with other antimicrobial wound dressing composite materials, the proposed BC/MGO nanocomposite has among the greatest antimicrobial property against broad-spectrum bacteria, making it a promising antimicrobial dressing in chronic wounds care.
32567254	7	58	dep	wounds	1556:1561	arg1	care					1563:1566	care	1563:1566	chronic wounds care	1548:1566	Compared with other antimicrobial wound dressing composite materials, the proposed BC/MGO nanocomposite has among the greatest antimicrobial property against broad-spectrum bacteria, making it a promising antimicrobial dressing in chronic wounds care.
32567254	2	59	theme	antimicrobial	326:338	arg1	dressing					340:347	a novel antimicrobial dressing	318:347	a novel antimicrobial dressing made from BC/methylglyoxal (MGO) composite with a dip-coating method inspired by naturally antimicrobial Manuka honey	318:465	In this paper, a novel antimicrobial dressing made from BC/methylglyoxal (MGO) composite with a dip-coating method inspired by naturally antimicrobial Manuka honey is proposed, which to our best knowledge, has not yet to be reported.
32567254	7	60	theme	proposed	1391:1398	arg1	nanocomposite					1407:1419	the proposed BC/MGO nanocomposite	1387:1419	the proposed BC/MGO nanocomposite	1387:1419	Compared with other antimicrobial wound dressing composite materials, the proposed BC/MGO nanocomposite has among the greatest antimicrobial property against broad-spectrum bacteria, making it a promising antimicrobial dressing in chronic wounds care.
32567254	1	61	theme	due	233:235	arg1	dressings					223:231	wound dressings	217:231	wound dressings due to the high water-holding capacity and great mechanical strength	217:300	Bacterial cellulose (BC) is a natural material produced by Acetobacter xylinum, widely used in wound dressings due to the high water-holding capacity and great mechanical strength.
32567254	4	62	theme	hydrogen	903:910	arg1	bonding					912:918	hydrogen bonding	903:918	hydrogen bonding	903:918	Thermal analysis indicates high temperature stability of both BC and BC/MGO, while compared with BC, BC/MGO exhibits slightly weaker thermal stability possibly due to reduction of hydrogen bonding and increase of crystallinity.
32567254	3	63	theme	MGO	663:665	arg1	nanostructure					639:651	the interconnected nanostructure	620:651	the interconnected nanostructure of BC and MGO	620:665	Characterizations by scanning electron microscope and atomic force microscopy show the interconnected nanostructure of BC and MGO and increase surface roughness of the BC/MGO composite.
32567254	3	64	theme	interconnected	624:637	arg1	nanostructure					639:651	the interconnected nanostructure	620:651	the interconnected nanostructure of BC and MGO	620:665	Characterizations by scanning electron microscope and atomic force microscopy show the interconnected nanostructure of BC and MGO and increase surface roughness of the BC/MGO composite.
32567254	2	65	theme	novel	320:324	arg1	dressing					340:347	a novel antimicrobial dressing	318:347	a novel antimicrobial dressing made from BC/methylglyoxal (MGO) composite with a dip-coating method inspired by naturally antimicrobial Manuka honey	318:465	In this paper, a novel antimicrobial dressing made from BC/methylglyoxal (MGO) composite with a dip-coating method inspired by naturally antimicrobial Manuka honey is proposed, which to our best knowledge, has not yet to be reported.
32567254	4	66	theme	Thermal	723:729	arg1	analysis					731:738	Thermal analysis	723:738	Thermal analysis	723:738	Thermal analysis indicates high temperature stability of both BC and BC/MGO, while compared with BC, BC/MGO exhibits slightly weaker thermal stability possibly due to reduction of hydrogen bonding and increase of crystallinity.
32567254	0	67	theme	Antimicrobial	92:104	arg1	Dressing					112:119	Broad-Spectrum Antimicrobial Wound Dressing	77:119	Broad-Spectrum Antimicrobial Wound Dressing	77:119	Nature-Inspired Bacterial Cellulose/Methylglyoxal (BC/MGO) Nanocomposite for Broad-Spectrum Antimicrobial Wound Dressing.
32567254	5	68	dep	BC	1010:1011	arg1	nanocomposite					1024:1036	nanocomposite	1024:1036	nanocomposite	1024:1036	Mechanical test confirms the strong mechanical property of BC and BC/MGO nanocomposite.
32567254	7	69	theme	other	1331:1335	arg1	materials					1376:1384	other antimicrobial wound dressing composite materials	1331:1384	other antimicrobial wound dressing composite materials	1331:1384	Compared with other antimicrobial wound dressing composite materials, the proposed BC/MGO nanocomposite has among the greatest antimicrobial property against broad-spectrum bacteria, making it a promising antimicrobial dressing in chronic wounds care.
32567254	2	70	theme	MGO	377:379	arg1	composite					382:390	BC/methylglyoxal (MGO) composite	359:390	BC/methylglyoxal (MGO) composite	359:390	In this paper, a novel antimicrobial dressing made from BC/methylglyoxal (MGO) composite with a dip-coating method inspired by naturally antimicrobial Manuka honey is proposed, which to our best knowledge, has not yet to be reported.
32567254	2	71	theme	best	493:496	arg1	knowledge					498:506	best knowledge	493:506	our best knowledge	489:506	In this paper, a novel antimicrobial dressing made from BC/methylglyoxal (MGO) composite with a dip-coating method inspired by naturally antimicrobial Manuka honey is proposed, which to our best knowledge, has not yet to be reported.
32567254	5	72	theme	BC/MGO	1017:1022	arg1	property					998:1005	the strong mechanical property	976:1005	the strong mechanical property of BC and BC/MGO nanocomposite	976:1036	Mechanical test confirms the strong mechanical property of BC and BC/MGO nanocomposite.
32567254	3	73	theme	electron	567:574	arg1	microscope					576:585	scanning electron microscope	558:585	scanning electron microscope	558:585	Characterizations by scanning electron microscope and atomic force microscopy show the interconnected nanostructure of BC and MGO and increase surface roughness of the BC/MGO composite.
32567254	1	74	theme	high	244:247	arg1	capacity					263:270	high water-holding capacity	244:270	high water-holding capacity	244:270	Bacterial cellulose (BC) is a natural material produced by Acetobacter xylinum, widely used in wound dressings due to the high water-holding capacity and great mechanical strength.
34450226	5	0	from	pH	1064:1065	arg1	48 h					1056:1059	48 h	1056:1059	48 h at pH 6.8	1056:1069	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	4	1	theme	@	676:676	arg1	GNRs					677:680	EV-CMCS@GNRs	669:680	EV-CMCS@GNRs	669:680	It was found that the loading and encapsulation rate of EV-CMCS and EV-CMCS@GNRs for PTX are 19.59~37.64% and 60.36~80.79% as well as 20.99~37.02% and 58.78~79.77%.
34450226	5	2	theme	accumulative	996:1007	arg1	amount					1017:1022	an accumulative release amount	993:1022	an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8	993:1069	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	5	2	theme	accumulative	996:1007	arg1	%					1031:1031	90.2%	1027:1031	90.2% for 24 h	1027:1040	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	5	2	theme	accumulative	996:1007	arg1	%					1050:1050	96.0%	1046:1050	96.0% for 48 h at pH 6.8	1046:1069	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	6	3	theme	paclitaxel	1205:1214	arg1	delivery					1216:1223	paclitaxel delivery	1205:1223	paclitaxel delivery	1205:1223	It deduced that the broken Schiff base under acidic condition can increase CMC of EV-CMCS@GNRs, which offered an alternative way for paclitaxel delivery for tumor therapy.
34450226	5	4	contain	have	824:827	arg1	it					821:822	it	821:822	it	821:822	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	5	4	contain	have	824:827	arg2	EV-CMCS					808:814	EV-CMCS	808:814	EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4	808:935	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	5	4	contain	have	824:827	arg2	%					843:843	18.7%	839:843	18.7%	839:843	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	5	4	contain	have	824:827	arg2	%					890:890	14.9%	886:890	14.9%	886:890	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	5	4	contain	have	824:827	arg2	%					833:833	11.5%	829:833	11.5%	829:833	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	6	5	theme	alternative	1185:1195	arg1	way					1197:1199	an alternative way	1182:1199	an alternative way for paclitaxel delivery for tumor therapy	1182:1241	It deduced that the broken Schiff base under acidic condition can increase CMC of EV-CMCS@GNRs, which offered an alternative way for paclitaxel delivery for tumor therapy.
34450226	3	6	theme	excellent	491:499	arg1	solubilization					501:514	excellent solubilization	491:514	excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL	491:598	Results demonstrated that both EV-CMCS and EV-CMCS@GNRs are nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL.
34450226	4	7	dep	loading	623:629	arg1	the					619:621	the	619:621	the	619:621	It was found that the loading and encapsulation rate of EV-CMCS and EV-CMCS@GNRs for PTX are 19.59~37.64% and 60.36~80.79% as well as 20.99~37.02% and 58.78~79.77%.
34450226	3	8	theme	micelle	529:535	arg1	structure					537:545	their micelle structure	523:545	their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL	523:598	Results demonstrated that both EV-CMCS and EV-CMCS@GNRs are nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL.
34450226	2	9	with	EV-CMCS	302:308	arg1	GNRs					324:327	GNRs	324:327	GNRs	324:327	EV-CMCS refluxed with GNRs for acquisition of EV-CMCS@GNRs nanocomposites for PTX Loading and release.
34450226	6	10	theme	acidic	1117:1122	arg1	condition					1124:1132	acidic condition	1117:1132	acidic condition	1117:1132	It deduced that the broken Schiff base under acidic condition can increase CMC of EV-CMCS@GNRs, which offered an alternative way for paclitaxel delivery for tumor therapy.
34450226	4	11	theme	GNRs	677:680	arg1	loading					623:629	loading	623:629	loading	623:629	It was found that the loading and encapsulation rate of EV-CMCS and EV-CMCS@GNRs for PTX are 19.59~37.64% and 60.36~80.79% as well as 20.99~37.02% and 58.78~79.77%.
34450226	4	11	theme	GNRs	677:680	arg1	rate					649:652	encapsulation rate	635:652	encapsulation rate	635:652	It was found that the loading and encapsulation rate of EV-CMCS and EV-CMCS@GNRs for PTX are 19.59~37.64% and 60.36~80.79% as well as 20.99~37.02% and 58.78~79.77%.
34450226	4	11	theme	GNRs	677:680	arg1	%					705:705	19.59~37.64%	694:705	19.59~37.64%	694:705	It was found that the loading and encapsulation rate of EV-CMCS and EV-CMCS@GNRs for PTX are 19.59~37.64% and 60.36~80.79% as well as 20.99~37.02% and 58.78~79.77%.
34450226	5	12	theme	accumulative	845:856	arg1	amount					866:871	accumulative release amount	845:871	accumulative release amount	845:871	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	3	13	theme	@	455:455	arg1	GNRs					456:459	both EV-CMCS and EV-CMCS@GNRs	431:459	GNRs	456:459	Results demonstrated that both EV-CMCS and EV-CMCS@GNRs are nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL.
34450226	3	13	theme	@	455:455	arg1	composites					475:484	nanoscale composites	465:484	nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL	465:598	Results demonstrated that both EV-CMCS and EV-CMCS@GNRs are nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL.
34450226	3	13	theme	@	455:455	arg1	EV-CMCS					436:442	both EV-CMCS and EV-CMCS@GNRs	431:459	EV-CMCS	436:442	Results demonstrated that both EV-CMCS and EV-CMCS@GNRs are nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL.
34450226	1	14	theme	Chitosan-ethyl	185:198	arg1	compound					219:226	Chitosan-ethyl vanillin (EV-CMCS) compound	185:226	Chitosan-ethyl vanillin (EV-CMCS) compound	185:226	Carboxylation chitosan achieved by alkalization linked with ethyl vanillin to obtain Chitosan-ethyl vanillin (EV-CMCS) compound through Schiff base reaction and confirmed by FT-IR, UV, XRD, TG and NMR.
34450226	1	15	theme	vanillin	200:207	arg1	compound					219:226	Chitosan-ethyl vanillin (EV-CMCS) compound	185:226	Chitosan-ethyl vanillin (EV-CMCS) compound	185:226	Carboxylation chitosan achieved by alkalization linked with ethyl vanillin to obtain Chitosan-ethyl vanillin (EV-CMCS) compound through Schiff base reaction and confirmed by FT-IR, UV, XRD, TG and NMR.
34450226	0	16	theme	responsive	3:12	arg1	release					14:20	pH responsive release	0:20	pH responsive release of paclitaxel by self-assembling Chitosan-ethyl vanillin@GNRs	0:82	pH responsive release of paclitaxel by self-assembling Chitosan-ethyl vanillin@GNRs nanocomposites.
34450226	3	17	theme	due	516:518	arg1	solubilization					501:514	excellent solubilization	491:514	excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL	491:598	Results demonstrated that both EV-CMCS and EV-CMCS@GNRs are nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL.
34450226	6	18	theme	Schiff	1099:1104	arg1	base					1106:1109	the broken Schiff base	1088:1109	the broken Schiff base under acidic condition	1088:1132	It deduced that the broken Schiff base under acidic condition can increase CMC of EV-CMCS@GNRs, which offered an alternative way for paclitaxel delivery for tumor therapy.
34450226	6	19	theme	tumor	1229:1233	arg1	therapy					1235:1241	tumor therapy	1229:1241	tumor therapy	1229:1241	It deduced that the broken Schiff base under acidic condition can increase CMC of EV-CMCS@GNRs, which offered an alternative way for paclitaxel delivery for tumor therapy.
34450226	3	20	with	composites	475:484	arg1	solubilization					501:514	excellent solubilization	491:514	excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL	491:598	Results demonstrated that both EV-CMCS and EV-CMCS@GNRs are nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL.
34450226	1	21	theme	Carboxylation	100:112	arg1	chitosan					114:121	Carboxylation chitosan	100:121	Carboxylation chitosan achieved by alkalization	100:146	Carboxylation chitosan achieved by alkalization linked with ethyl vanillin to obtain Chitosan-ethyl vanillin (EV-CMCS) compound through Schiff base reaction and confirmed by FT-IR, UV, XRD, TG and NMR.
34450226	2	22	theme	GNRs	356:359	arg1	acquisition					333:343	acquisition	333:343	acquisition of EV-CMCS@GNRs	333:359	EV-CMCS refluxed with GNRs for acquisition of EV-CMCS@GNRs nanocomposites for PTX Loading and release.
34450226	5	23	theme	%	1050:1050	arg1	amount					1017:1022	an accumulative release amount	993:1022	an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8	993:1069	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	5	23	theme	%	1050:1050	arg1	%					1031:1031	90.2%	1027:1031	90.2% for 24 h	1027:1040	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	5	23	theme	%	1050:1050	arg1	%					1050:1050	96.0%	1046:1050	96.0% for 48 h at pH 6.8	1046:1069	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	5	24	dep	%	833:833	arg1	amount					866:871	accumulative release amount	845:871	accumulative release amount	845:871	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	3	25	theme	EV-CMCS	448:454	arg1	GNRs					456:459	both EV-CMCS and EV-CMCS@GNRs	431:459	GNRs	456:459	Results demonstrated that both EV-CMCS and EV-CMCS@GNRs are nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL.
34450226	3	25	theme	EV-CMCS	448:454	arg1	composites					475:484	nanoscale composites	465:484	nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL	465:598	Results demonstrated that both EV-CMCS and EV-CMCS@GNRs are nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL.
34450226	3	25	theme	EV-CMCS	448:454	arg1	EV-CMCS					436:442	both EV-CMCS and EV-CMCS@GNRs	431:459	EV-CMCS	436:442	Results demonstrated that both EV-CMCS and EV-CMCS@GNRs are nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL.
34450226	3	26	theme	CMC	554:556	arg1	values					558:563	CMC values	554:563	CMC values of 0.06683 mg/mL and 0.06537 mg/mL	554:598	Results demonstrated that both EV-CMCS and EV-CMCS@GNRs are nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL.
34450226	1	27	theme	ethyl	160:164	arg1	vanillin					166:173	ethyl vanillin	160:173	ethyl vanillin	160:173	Carboxylation chitosan achieved by alkalization linked with ethyl vanillin to obtain Chitosan-ethyl vanillin (EV-CMCS) compound through Schiff base reaction and confirmed by FT-IR, UV, XRD, TG and NMR.
34450226	5	28	theme	release	858:864	arg1	amount					866:871	accumulative release amount	845:871	accumulative release amount	845:871	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	5	29	theme	sudden	965:970	arg1	release					972:978	sudden release	965:978	sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8	965:1069	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	3	30	theme	0.06537 mg/mL	586:598	arg1	values					558:563	CMC values	554:563	CMC values of 0.06683 mg/mL and 0.06537 mg/mL	554:598	Results demonstrated that both EV-CMCS and EV-CMCS@GNRs are nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL.
34450226	1	31	theme	EV-CMCS	210:216	arg1	compound					219:226	Chitosan-ethyl vanillin (EV-CMCS) compound	185:226	Chitosan-ethyl vanillin (EV-CMCS) compound	185:226	Carboxylation chitosan achieved by alkalization linked with ethyl vanillin to obtain Chitosan-ethyl vanillin (EV-CMCS) compound through Schiff base reaction and confirmed by FT-IR, UV, XRD, TG and NMR.
34450226	4	32	theme	encapsulation	635:647	arg1	loading					623:629	loading	623:629	loading	623:629	It was found that the loading and encapsulation rate of EV-CMCS and EV-CMCS@GNRs for PTX are 19.59~37.64% and 60.36~80.79% as well as 20.99~37.02% and 58.78~79.77%.
34450226	4	32	theme	encapsulation	635:647	arg1	rate					649:652	encapsulation rate	635:652	encapsulation rate	635:652	It was found that the loading and encapsulation rate of EV-CMCS and EV-CMCS@GNRs for PTX are 19.59~37.64% and 60.36~80.79% as well as 20.99~37.02% and 58.78~79.77%.
34450226	4	32	theme	encapsulation	635:647	arg1	%					705:705	19.59~37.64%	694:705	19.59~37.64%	694:705	It was found that the loading and encapsulation rate of EV-CMCS and EV-CMCS@GNRs for PTX are 19.59~37.64% and 60.36~80.79% as well as 20.99~37.02% and 58.78~79.77%.
34450226	2	33	theme	PTX	380:382	arg1	Loading					384:390	PTX Loading	380:390	PTX Loading	380:390	EV-CMCS refluxed with GNRs for acquisition of EV-CMCS@GNRs nanocomposites for PTX Loading and release.
34450226	5	34	theme	%	1031:1031	arg1	amount					1017:1022	an accumulative release amount	993:1022	an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8	993:1069	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	5	34	theme	%	1031:1031	arg1	%					1031:1031	90.2%	1027:1031	90.2% for 24 h	1027:1040	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	5	34	theme	%	1031:1031	arg1	%					1050:1050	96.0%	1046:1050	96.0% for 48 h at pH 6.8	1046:1069	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	0	35	theme	paclitaxel	25:34	arg1	release					14:20	pH responsive release	0:20	pH responsive release of paclitaxel by self-assembling Chitosan-ethyl vanillin@GNRs	0:82	pH responsive release of paclitaxel by self-assembling Chitosan-ethyl vanillin@GNRs nanocomposites.
34450226	1	36	theme	Schiff	236:241	arg1	reaction					248:255	Schiff base reaction	236:255	Schiff base reaction	236:255	Carboxylation chitosan achieved by alkalization linked with ethyl vanillin to obtain Chitosan-ethyl vanillin (EV-CMCS) compound through Schiff base reaction and confirmed by FT-IR, UV, XRD, TG and NMR.
34450226	0	37	theme	Chitosan-ethyl	55:68	arg1	GNRs					79:82	self-assembling Chitosan-ethyl vanillin@GNRs	39:82	self-assembling Chitosan-ethyl vanillin@GNRs	39:82	pH responsive release of paclitaxel by self-assembling Chitosan-ethyl vanillin@GNRs nanocomposites.
34450226	6	38	theme	@	1161:1161	arg1	GNRs					1162:1165	EV-CMCS@GNRs	1154:1165	EV-CMCS@GNRs	1154:1165	It deduced that the broken Schiff base under acidic condition can increase CMC of EV-CMCS@GNRs, which offered an alternative way for paclitaxel delivery for tumor therapy.
34450226	3	39	theme	0.06683 mg/mL	568:580	arg1	values					558:563	CMC values	554:563	CMC values of 0.06683 mg/mL and 0.06537 mg/mL	554:598	Results demonstrated that both EV-CMCS and EV-CMCS@GNRs are nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL.
34450226	3	40	theme	nanoscale	465:473	arg1	GNRs					456:459	both EV-CMCS and EV-CMCS@GNRs	431:459	GNRs	456:459	Results demonstrated that both EV-CMCS and EV-CMCS@GNRs are nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL.
34450226	3	40	theme	nanoscale	465:473	arg1	composites					475:484	nanoscale composites	465:484	nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL	465:598	Results demonstrated that both EV-CMCS and EV-CMCS@GNRs are nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL.
34450226	3	40	theme	nanoscale	465:473	arg1	EV-CMCS					436:442	both EV-CMCS and EV-CMCS@GNRs	431:459	EV-CMCS	436:442	Results demonstrated that both EV-CMCS and EV-CMCS@GNRs are nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL.
34450226	0	41	theme	self-assembling	39:53	arg1	GNRs					79:82	self-assembling Chitosan-ethyl vanillin@GNRs	39:82	self-assembling Chitosan-ethyl vanillin@GNRs	39:82	pH responsive release of paclitaxel by self-assembling Chitosan-ethyl vanillin@GNRs nanocomposites.
34450226	6	42	theme	EV-CMCS	1154:1160	arg1	GNRs					1162:1165	EV-CMCS@GNRs	1154:1165	EV-CMCS@GNRs	1154:1165	It deduced that the broken Schiff base under acidic condition can increase CMC of EV-CMCS@GNRs, which offered an alternative way for paclitaxel delivery for tumor therapy.
34450226	4	43	theme	EV-CMCS	657:663	arg1	loading					623:629	loading	623:629	loading	623:629	It was found that the loading and encapsulation rate of EV-CMCS and EV-CMCS@GNRs for PTX are 19.59~37.64% and 60.36~80.79% as well as 20.99~37.02% and 58.78~79.77%.
34450226	4	43	theme	EV-CMCS	657:663	arg1	rate					649:652	encapsulation rate	635:652	encapsulation rate	635:652	It was found that the loading and encapsulation rate of EV-CMCS and EV-CMCS@GNRs for PTX are 19.59~37.64% and 60.36~80.79% as well as 20.99~37.02% and 58.78~79.77%.
34450226	4	43	theme	EV-CMCS	657:663	arg1	%					705:705	19.59~37.64%	694:705	19.59~37.64%	694:705	It was found that the loading and encapsulation rate of EV-CMCS and EV-CMCS@GNRs for PTX are 19.59~37.64% and 60.36~80.79% as well as 20.99~37.02% and 58.78~79.77%.
34450226	5	44	theme	EV-CMCS	942:948	arg1	GNRs					950:953	the EV-CMCS@GNRs	938:953	the EV-CMCS@GNRs	938:953	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	4	45	theme	EV-CMCS	669:675	arg1	GNRs					677:680	EV-CMCS@GNRs	669:680	EV-CMCS@GNRs	669:680	It was found that the loading and encapsulation rate of EV-CMCS and EV-CMCS@GNRs for PTX are 19.59~37.64% and 60.36~80.79% as well as 20.99~37.02% and 58.78~79.77%.
34450226	6	46	theme	GNRs	1162:1165	arg1	CMC					1147:1149	CMC	1147:1149	CMC	1147:1149	It deduced that the broken Schiff base under acidic condition can increase CMC of EV-CMCS@GNRs, which offered an alternative way for paclitaxel delivery for tumor therapy.
34450226	1	47	theme	base	243:246	arg1	reaction					248:255	Schiff base reaction	236:255	Schiff base reaction	236:255	Carboxylation chitosan achieved by alkalization linked with ethyl vanillin to obtain Chitosan-ethyl vanillin (EV-CMCS) compound through Schiff base reaction and confirmed by FT-IR, UV, XRD, TG and NMR.
34450226	5	48	theme	@	949:949	arg1	GNRs					950:953	the EV-CMCS@GNRs	938:953	the EV-CMCS@GNRs	938:953	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	2	49	theme	refluxed	310:317	arg1	EV-CMCS					302:308	EV-CMCS refluxed	302:317	EV-CMCS refluxed with GNRs for acquisition of EV-CMCS@GNRs	302:359	EV-CMCS refluxed with GNRs for acquisition of EV-CMCS@GNRs nanocomposites for PTX Loading and release.
34450226	0	50	theme	@	78:78	arg1	GNRs					79:82	self-assembling Chitosan-ethyl vanillin@GNRs	39:82	self-assembling Chitosan-ethyl vanillin@GNRs	39:82	pH responsive release of paclitaxel by self-assembling Chitosan-ethyl vanillin@GNRs nanocomposites.
34450226	5	51	theme	EV-CMCS	808:814	arg1	release					797:803	only the delayed release	780:803	only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4	780:935	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	5	52	theme	delayed	789:795	arg1	release					797:803	only the delayed release	780:803	only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4	780:935	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	2	53	theme	@	355:355	arg1	GNRs					356:359	EV-CMCS@GNRs	348:359	EV-CMCS@GNRs	348:359	EV-CMCS refluxed with GNRs for acquisition of EV-CMCS@GNRs nanocomposites for PTX Loading and release.
34450226	5	54	contain	have	988:991	arg2	%					1031:1031	90.2%	1027:1031	90.2% for 24 h	1027:1040	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	5	54	contain	have	988:991	arg1	it					985:986	it	985:986	it	985:986	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	5	54	contain	have	988:991	arg2	release					972:978	sudden release	965:978	sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8	965:1069	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	5	54	contain	have	988:991	arg2	amount					1017:1022	an accumulative release amount	993:1022	an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8	993:1069	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	5	54	contain	have	988:991	arg2	%					1050:1050	96.0%	1046:1050	96.0% for 48 h at pH 6.8	1046:1069	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	0	55	theme	vanillin	70:77	arg1	GNRs					79:82	self-assembling Chitosan-ethyl vanillin@GNRs	39:82	self-assembling Chitosan-ethyl vanillin@GNRs	39:82	pH responsive release of paclitaxel by self-assembling Chitosan-ethyl vanillin@GNRs nanocomposites.
34450226	5	56	theme	release	1009:1015	arg1	amount					1017:1022	an accumulative release amount	993:1022	an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8	993:1069	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	5	56	theme	release	1009:1015	arg1	%					1031:1031	90.2%	1027:1031	90.2% for 24 h	1027:1040	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	5	56	theme	release	1009:1015	arg1	%					1050:1050	96.0%	1046:1050	96.0% for 48 h at pH 6.8	1046:1069	Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8.
34450226	6	57	theme	broken	1092:1097	arg1	base					1106:1109	the broken Schiff base	1088:1109	the broken Schiff base under acidic condition	1088:1132	It deduced that the broken Schiff base under acidic condition can increase CMC of EV-CMCS@GNRs, which offered an alternative way for paclitaxel delivery for tumor therapy.
34450226	2	58	theme	EV-CMCS	348:354	arg1	GNRs					356:359	EV-CMCS@GNRs	348:359	EV-CMCS@GNRs	348:359	EV-CMCS refluxed with GNRs for acquisition of EV-CMCS@GNRs nanocomposites for PTX Loading and release.
34574138	3	0	theme	amino	328:332	arg1	sequences					339:347	two major amino acid sequences	318:347	two major amino acid sequences with different modifications, including glycosylation, phosphorylation and oxidation	318:432	CMP has two major amino acid sequences with different modifications, including glycosylation, phosphorylation and oxidation.
34574138	1	1	attach	released	130:137	arg2	CMP					122:124	CMP	122:124	CMP	122:124	Caseinomacropeptide (CMP) is released from bovine kappa-casein after rennet treatment and is one of the major peptides in whey protein isolate.
34574138	1	1	attach	released	130:137	arg1	kappa-casein					151:162	bovine kappa-casein	144:162	bovine kappa-casein	144:162	Caseinomacropeptide (CMP) is released from bovine kappa-casein after rennet treatment and is one of the major peptides in whey protein isolate.
34574138	1	1	attach	released	130:137	arg2	Caseinomacropeptide					101:119	Caseinomacropeptide	101:119	Caseinomacropeptide (CMP)	101:125	Caseinomacropeptide (CMP) is released from bovine kappa-casein after rennet treatment and is one of the major peptides in whey protein isolate.
34574138	1	2	from	one	194:196	arg1	isolate					236:242	whey protein isolate	223:242	whey protein isolate	223:242	Caseinomacropeptide (CMP) is released from bovine kappa-casein after rennet treatment and is one of the major peptides in whey protein isolate.
34574138	9	3	theme	product	1415:1421	arg1	quality					1423:1429	product quality	1415:1429	product quality	1415:1429	Overall, this novel approach provides comprehensive characterization of CMP and CMP-derived peptides and glycopeptides, and it can be applied in future studies of product quality, digestive survival and bioactivity.
34574138	2	4	contain	has	249:251	arg1	CMP					245:247	CMP	245:247	CMP	245:247	CMP has in vitro anti-inflammatory and antibacterial activities.
34574138	2	4	contain	has	249:251	arg2	activities					298:307	in vitro anti-inflammatory and antibacterial activities	253:307	in vitro anti-inflammatory and antibacterial activities	253:307	CMP has in vitro anti-inflammatory and antibacterial activities.
34574138	3	5	theme	acid	334:337	arg1	sequences					339:347	two major amino acid sequences	318:347	two major amino acid sequences with different modifications, including glycosylation, phosphorylation and oxidation	318:432	CMP has two major amino acid sequences with different modifications, including glycosylation, phosphorylation and oxidation.
34574138	9	6	theme	CMP-derived	1332:1342	arg1	peptides					1344:1351	CMP-derived peptides	1332:1351	CMP-derived peptides	1332:1351	Overall, this novel approach provides comprehensive characterization of CMP and CMP-derived peptides and glycopeptides, and it can be applied in future studies of product quality, digestive survival and bioactivity.
34574138	9	7	gly	glycopeptides	1357:1369	arg1	CMP					1324:1326	CMP	1324:1326	CMP	1324:1326	Overall, this novel approach provides comprehensive characterization of CMP and CMP-derived peptides and glycopeptides, and it can be applied in future studies of product quality, digestive survival and bioactivity.
34574138	9	7	gly	glycopeptides	1357:1369	arg2	glycopeptides					1357:1369	glycopeptides	1357:1369	glycopeptides	1357:1369	Overall, this novel approach provides comprehensive characterization of CMP and CMP-derived peptides and glycopeptides, and it can be applied in future studies of product quality, digestive survival and bioactivity.
34574138	9	7	gly	glycopeptides	1357:1369	arg1	peptides					1344:1351	CMP-derived peptides	1332:1351	CMP-derived peptides	1332:1351	Overall, this novel approach provides comprehensive characterization of CMP and CMP-derived peptides and glycopeptides, and it can be applied in future studies of product quality, digestive survival and bioactivity.
34574138	8	8	dep	identified	1147:1156	arg1	CMP					1179:1181	one CMP standard	1175:1190	one CMP standard	1175:1190	Fifty-one intact CMPs and 159 CMP-derived peptides were identified in four samples (one CMP standard, two commercial CMP products and one whey protein isolate).
34574138	8	9	theme	CMP-derived	1121:1131	arg1	peptides					1133:1140	159 CMP-derived peptides	1117:1140	159 CMP-derived peptides	1117:1140	Fifty-one intact CMPs and 159 CMP-derived peptides were identified in four samples (one CMP standard, two commercial CMP products and one whey protein isolate).
34574138	8	10	theme	intact	1101:1106	arg1	CMPs					1108:1111	Fifty-one intact CMPs	1091:1111	Fifty-one intact CMPs	1091:1111	Fifty-one intact CMPs and 159 CMP-derived peptides were identified in four samples (one CMP standard, two commercial CMP products and one whey protein isolate).
34574138	7	11	theme	peptide	978:984	arg1	sequence					986:993	peptide sequence	978:993	peptide sequence	978:993	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra of CMPs were annotated to confirm peptide sequence, glycan composition and other post-translational modifications using automatic data processing.
34574138	4	12	theme	intact	501:506	arg1	CMP					508:510	intact CMP	501:510	intact CMP	501:510	However, no previous work has provided a comprehensive profile of intact CMP.
34574138	6	13	theme	electron-transfer/higher-energy	818:848	arg1	dissociation					860:871	electron-transfer/higher-energy collision dissociation	818:871	electron-transfer/higher-energy collision dissociation	818:871	In this study, we developed a top-down glycopeptidomics-based analytical method to profile CMP and CMP-derived peptides using Orbitrap mass spectrometry combined with nano-liquid chromatography with electron-transfer/higher-energy collision dissociation.
34574138	7	14	theme	data	1074:1077	arg1	processing					1079:1088	automatic data processing	1064:1088	automatic data processing	1064:1088	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra of CMPs were annotated to confirm peptide sequence, glycan composition and other post-translational modifications using automatic data processing.
34574138	3	15	contain	has	314:316	arg1	CMP					310:312	CMP	310:312	CMP	310:312	CMP has two major amino acid sequences with different modifications, including glycosylation, phosphorylation and oxidation.
34574138	3	15	contain	has	314:316	arg2	sequences					339:347	two major amino acid sequences	318:347	two major amino acid sequences with different modifications, including glycosylation, phosphorylation and oxidation	318:432	CMP has two major amino acid sequences with different modifications, including glycosylation, phosphorylation and oxidation.
34574138	3	16	theme	different	354:362	arg1	phosphorylation					404:418	phosphorylation	404:418	phosphorylation	404:418	CMP has two major amino acid sequences with different modifications, including glycosylation, phosphorylation and oxidation.
34574138	3	16	theme	different	354:362	arg1	modifications					364:376	different modifications	354:376	different modifications	354:376	CMP has two major amino acid sequences with different modifications, including glycosylation, phosphorylation and oxidation.
34574138	3	16	theme	different	354:362	arg1	glycosylation					389:401	glycosylation	389:401	glycosylation	389:401	CMP has two major amino acid sequences with different modifications, including glycosylation, phosphorylation and oxidation.
34574138	3	16	theme	different	354:362	arg1	oxidation					424:432	oxidation	424:432	oxidation	424:432	CMP has two major amino acid sequences with different modifications, including glycosylation, phosphorylation and oxidation.
34574138	5	17	theme	Full	513:516	arg1	characterization					518:533	Full characterization	513:533	Full characterization of CMP composition and structure	513:566	Full characterization of CMP composition and structure is essential to understand the bioactivity of CMP.
34574138	2	18	theme	antibacterial	284:296	arg1	activities					298:307	in vitro anti-inflammatory and antibacterial activities	253:307	in vitro anti-inflammatory and antibacterial activities	253:307	CMP has in vitro anti-inflammatory and antibacterial activities.
34574138	6	19	theme	Orbitrap	745:752	arg1	spectrometry					759:770	Orbitrap mass spectrometry	745:770	Orbitrap mass spectrometry combined with nano-liquid chromatography with electron-transfer/higher-energy collision dissociation	745:871	In this study, we developed a top-down glycopeptidomics-based analytical method to profile CMP and CMP-derived peptides using Orbitrap mass spectrometry combined with nano-liquid chromatography with electron-transfer/higher-energy collision dissociation.
34574138	2	20	dep	in	253:254	arg1	vitro					256:260	vitro	256:260	vitro	256:260	CMP has in vitro anti-inflammatory and antibacterial activities.
34574138	8	21	link	CMP-derived	1121:1131	arg1	peptides					1133:1140	159 CMP-derived peptides	1117:1140	159 CMP-derived peptides	1117:1140	Fifty-one intact CMPs and 159 CMP-derived peptides were identified in four samples (one CMP standard, two commercial CMP products and one whey protein isolate).
34574138	9	22	theme	CMP	1324:1326	arg1	characterization					1304:1319	comprehensive characterization	1290:1319	comprehensive characterization of CMP and CMP-derived peptides	1290:1351	Overall, this novel approach provides comprehensive characterization of CMP and CMP-derived peptides and glycopeptides, and it can be applied in future studies of product quality, digestive survival and bioactivity.
34574138	9	22	theme	CMP	1324:1326	arg1	glycopeptides					1357:1369	glycopeptides	1357:1369	glycopeptides	1357:1369	Overall, this novel approach provides comprehensive characterization of CMP and CMP-derived peptides and glycopeptides, and it can be applied in future studies of product quality, digestive survival and bioactivity.
34574138	7	23	theme	CMPs	947:950	arg1	spectra					936:942	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra	874:942	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra of CMPs	874:950	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra of CMPs were annotated to confirm peptide sequence, glycan composition and other post-translational modifications using automatic data processing.
34574138	6	24	with	chromatography	798:811	arg1	dissociation					860:871	electron-transfer/higher-energy collision dissociation	818:871	electron-transfer/higher-energy collision dissociation	818:871	In this study, we developed a top-down glycopeptidomics-based analytical method to profile CMP and CMP-derived peptides using Orbitrap mass spectrometry combined with nano-liquid chromatography with electron-transfer/higher-energy collision dissociation.
34574138	4	25	theme	CMP	508:510	arg1	profile					490:496	a comprehensive profile	474:496	a comprehensive profile of intact CMP	474:510	However, no previous work has provided a comprehensive profile of intact CMP.
34574138	6	26	link	CMP-derived	718:728	arg1	peptides					730:737	CMP-derived peptides	718:737	CMP-derived peptides	718:737	In this study, we developed a top-down glycopeptidomics-based analytical method to profile CMP and CMP-derived peptides using Orbitrap mass spectrometry combined with nano-liquid chromatography with electron-transfer/higher-energy collision dissociation.
34574138	7	27	theme	automatic	1064:1072	arg1	processing					1079:1088	automatic data processing	1064:1088	automatic data processing	1064:1088	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra of CMPs were annotated to confirm peptide sequence, glycan composition and other post-translational modifications using automatic data processing.
34574138	5	28	theme	CMP	538:540	arg1	characterization					518:533	Full characterization	513:533	Full characterization of CMP composition and structure	513:566	Full characterization of CMP composition and structure is essential to understand the bioactivity of CMP.
34574138	9	29	theme	digestive	1432:1440	arg1	survival					1442:1449	digestive survival	1432:1449	digestive survival	1432:1449	Overall, this novel approach provides comprehensive characterization of CMP and CMP-derived peptides and glycopeptides, and it can be applied in future studies of product quality, digestive survival and bioactivity.
34574138	7	30	theme	spectrometry	912:923	arg1	spectra					936:942	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra	874:942	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra of CMPs	874:950	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra of CMPs were annotated to confirm peptide sequence, glycan composition and other post-translational modifications using automatic data processing.
34574138	8	31	theme	standard	1183:1190	arg1	CMP					1179:1181	one CMP standard	1175:1190	one CMP standard	1175:1190	Fifty-one intact CMPs and 159 CMP-derived peptides were identified in four samples (one CMP standard, two commercial CMP products and one whey protein isolate).
34574138	5	32	theme	CMP	614:616	arg1	bioactivity					599:609	the bioactivity	595:609	the bioactivity of CMP	595:616	Full characterization of CMP composition and structure is essential to understand the bioactivity of CMP.
34574138	7	33	theme	glycan	996:1001	arg1	composition					1003:1013	glycan composition	996:1013	glycan composition	996:1013	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra of CMPs were annotated to confirm peptide sequence, glycan composition and other post-translational modifications using automatic data processing.
34574138	6	34	theme	CMP-derived	718:728	arg1	peptides					730:737	CMP-derived peptides	718:737	CMP-derived peptides	718:737	In this study, we developed a top-down glycopeptidomics-based analytical method to profile CMP and CMP-derived peptides using Orbitrap mass spectrometry combined with nano-liquid chromatography with electron-transfer/higher-energy collision dissociation.
34574138	9	35	link	CMP-derived	1332:1342	arg1	peptides					1344:1351	CMP-derived peptides	1332:1351	CMP-derived peptides	1332:1351	Overall, this novel approach provides comprehensive characterization of CMP and CMP-derived peptides and glycopeptides, and it can be applied in future studies of product quality, digestive survival and bioactivity.
34574138	0	36	theme	Kappa-Casein	19:30	arg1	Glycomacropeptide					32:48	Bovine Kappa-Casein Glycomacropeptide	12:48	Bovine Kappa-Casein Glycomacropeptide	12:48	Analysis of Bovine Kappa-Casein Glycomacropeptide by Liquid Chromatography-Tandem Mass Spectrometry.
34574138	3	37	with	sequences	339:347	arg1	phosphorylation					404:418	phosphorylation	404:418	phosphorylation	404:418	CMP has two major amino acid sequences with different modifications, including glycosylation, phosphorylation and oxidation.
34574138	3	37	with	sequences	339:347	arg1	modifications					364:376	different modifications	354:376	different modifications	354:376	CMP has two major amino acid sequences with different modifications, including glycosylation, phosphorylation and oxidation.
34574138	3	37	with	sequences	339:347	arg1	glycosylation					389:401	glycosylation	389:401	glycosylation	389:401	CMP has two major amino acid sequences with different modifications, including glycosylation, phosphorylation and oxidation.
34574138	3	37	with	sequences	339:347	arg1	oxidation					424:432	oxidation	424:432	oxidation	424:432	CMP has two major amino acid sequences with different modifications, including glycosylation, phosphorylation and oxidation.
34574138	8	38	theme	commercial	1197:1206	arg1	products					1212:1219	two commercial CMP products	1193:1219	two commercial CMP products	1193:1219	Fifty-one intact CMPs and 159 CMP-derived peptides were identified in four samples (one CMP standard, two commercial CMP products and one whey protein isolate).
34574138	9	39	theme	peptides	1344:1351	arg1	characterization					1304:1319	comprehensive characterization	1290:1319	comprehensive characterization of CMP and CMP-derived peptides	1290:1351	Overall, this novel approach provides comprehensive characterization of CMP and CMP-derived peptides and glycopeptides, and it can be applied in future studies of product quality, digestive survival and bioactivity.
34574138	9	39	theme	peptides	1344:1351	arg1	glycopeptides					1357:1369	glycopeptides	1357:1369	glycopeptides	1357:1369	Overall, this novel approach provides comprehensive characterization of CMP and CMP-derived peptides and glycopeptides, and it can be applied in future studies of product quality, digestive survival and bioactivity.
34574138	1	40	theme	major	205:209	arg1	peptides					211:218	the major peptides	201:218	the major peptides	201:218	Caseinomacropeptide (CMP) is released from bovine kappa-casein after rennet treatment and is one of the major peptides in whey protein isolate.
34574138	0	41	theme	Bovine	12:17	arg1	Glycomacropeptide					32:48	Bovine Kappa-Casein Glycomacropeptide	12:48	Bovine Kappa-Casein Glycomacropeptide	12:48	Analysis of Bovine Kappa-Casein Glycomacropeptide by Liquid Chromatography-Tandem Mass Spectrometry.
34574138	7	42	theme	chromatography-tandem	885:905	arg1	LC-MS/MS					926:933	LC-MS/MS	926:933	LC-MS/MS	926:933	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra of CMPs were annotated to confirm peptide sequence, glycan composition and other post-translational modifications using automatic data processing.
34574138	7	42	theme	chromatography-tandem	885:905	arg1	spectrometry					912:923	liquid chromatography-tandem mass spectrometry	878:923	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra of CMPs	874:950	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra of CMPs were annotated to confirm peptide sequence, glycan composition and other post-translational modifications using automatic data processing.
34574138	9	43	theme	survival	1442:1449	arg1	studies					1404:1410	future studies	1397:1410	future studies of product quality, digestive survival and bioactivity	1397:1465	Overall, this novel approach provides comprehensive characterization of CMP and CMP-derived peptides and glycopeptides, and it can be applied in future studies of product quality, digestive survival and bioactivity.
34574138	1	44	theme	peptides	211:218	arg1	peptides					211:218	the major peptides	201:218	the major peptides	201:218	Caseinomacropeptide (CMP) is released from bovine kappa-casein after rennet treatment and is one of the major peptides in whey protein isolate.
34574138	1	44	theme	peptides	211:218	arg1	one					194:196	one	194:196	one	194:196	Caseinomacropeptide (CMP) is released from bovine kappa-casein after rennet treatment and is one of the major peptides in whey protein isolate.
34574138	7	45	theme	mass	907:910	arg1	LC-MS/MS					926:933	LC-MS/MS	926:933	LC-MS/MS	926:933	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra of CMPs were annotated to confirm peptide sequence, glycan composition and other post-translational modifications using automatic data processing.
34574138	7	45	theme	mass	907:910	arg1	spectrometry					912:923	liquid chromatography-tandem mass spectrometry	878:923	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra of CMPs	874:950	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra of CMPs were annotated to confirm peptide sequence, glycan composition and other post-translational modifications using automatic data processing.
34574138	9	46	theme	quality	1423:1429	arg1	studies					1404:1410	future studies	1397:1410	future studies of product quality, digestive survival and bioactivity	1397:1465	Overall, this novel approach provides comprehensive characterization of CMP and CMP-derived peptides and glycopeptides, and it can be applied in future studies of product quality, digestive survival and bioactivity.
34574138	6	47	theme	profile	702:708	arg1	CMP					710:712	profile CMP	702:712	profile CMP	702:712	In this study, we developed a top-down glycopeptidomics-based analytical method to profile CMP and CMP-derived peptides using Orbitrap mass spectrometry combined with nano-liquid chromatography with electron-transfer/higher-energy collision dissociation.
34574138	9	48	theme	bioactivity	1455:1465	arg1	studies					1404:1410	future studies	1397:1410	future studies of product quality, digestive survival and bioactivity	1397:1465	Overall, this novel approach provides comprehensive characterization of CMP and CMP-derived peptides and glycopeptides, and it can be applied in future studies of product quality, digestive survival and bioactivity.
34574138	0	49	theme	Glycomacropeptide	32:48	arg1	Analysis					0:7	Analysis	0:7	Analysis of Bovine Kappa-Casein Glycomacropeptide by Liquid Chromatography-Tandem Mass Spectrometry.	0:99	Analysis of Bovine Kappa-Casein Glycomacropeptide by Liquid Chromatography-Tandem Mass Spectrometry.
34574138	8	50	dep	CMP	1179:1181	arg1	isolate					1242:1248	one whey protein isolate	1225:1248	one whey protein isolate	1225:1248	Fifty-one intact CMPs and 159 CMP-derived peptides were identified in four samples (one CMP standard, two commercial CMP products and one whey protein isolate).
34574138	8	50	dep	CMP	1179:1181	arg1	products					1212:1219	two commercial CMP products	1193:1219	two commercial CMP products	1193:1219	Fifty-one intact CMPs and 159 CMP-derived peptides were identified in four samples (one CMP standard, two commercial CMP products and one whey protein isolate).
34574138	8	51	theme	CMP	1208:1210	arg1	products					1212:1219	two commercial CMP products	1193:1219	two commercial CMP products	1193:1219	Fifty-one intact CMPs and 159 CMP-derived peptides were identified in four samples (one CMP standard, two commercial CMP products and one whey protein isolate).
34574138	6	52	theme	collision	850:858	arg1	dissociation					860:871	electron-transfer/higher-energy collision dissociation	818:871	electron-transfer/higher-energy collision dissociation	818:871	In this study, we developed a top-down glycopeptidomics-based analytical method to profile CMP and CMP-derived peptides using Orbitrap mass spectrometry combined with nano-liquid chromatography with electron-transfer/higher-energy collision dissociation.
34574138	1	53	theme	whey	223:226	arg1	isolate					236:242	whey protein isolate	223:242	whey protein isolate	223:242	Caseinomacropeptide (CMP) is released from bovine kappa-casein after rennet treatment and is one of the major peptides in whey protein isolate.
34574138	0	54	theme	Chromatography-Tandem	60:80	arg1	Spectrometry					87:98	Liquid Chromatography-Tandem Mass Spectrometry	53:98	Liquid Chromatography-Tandem Mass Spectrometry	53:98	Analysis of Bovine Kappa-Casein Glycomacropeptide by Liquid Chromatography-Tandem Mass Spectrometry.
34574138	7	55	theme	liquid	878:883	arg1	LC-MS/MS					926:933	LC-MS/MS	926:933	LC-MS/MS	926:933	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra of CMPs were annotated to confirm peptide sequence, glycan composition and other post-translational modifications using automatic data processing.
34574138	7	55	theme	liquid	878:883	arg1	spectrometry					912:923	liquid chromatography-tandem mass spectrometry	878:923	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra of CMPs	874:950	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra of CMPs were annotated to confirm peptide sequence, glycan composition and other post-translational modifications using automatic data processing.
34574138	4	56	theme	comprehensive	476:488	arg1	profile					490:496	a comprehensive profile	474:496	a comprehensive profile of intact CMP	474:510	However, no previous work has provided a comprehensive profile of intact CMP.
34574138	5	57	dep	CMP	538:540	arg1	structure					558:566	structure	558:566	structure	558:566	Full characterization of CMP composition and structure is essential to understand the bioactivity of CMP.
34574138	5	57	dep	CMP	538:540	arg1	composition					542:552	composition	542:552	composition	542:552	Full characterization of CMP composition and structure is essential to understand the bioactivity of CMP.
34574138	5	57	dep	CMP	538:540	arg1	CMP					538:540	CMP composition and structure	538:566	CMP composition and structure	538:566	Full characterization of CMP composition and structure is essential to understand the bioactivity of CMP.
34574138	0	58	theme	Liquid	53:58	arg1	Spectrometry					87:98	Liquid Chromatography-Tandem Mass Spectrometry	53:98	Liquid Chromatography-Tandem Mass Spectrometry	53:98	Analysis of Bovine Kappa-Casein Glycomacropeptide by Liquid Chromatography-Tandem Mass Spectrometry.
34574138	3	59	theme	major	322:326	arg1	sequences					339:347	two major amino acid sequences	318:347	two major amino acid sequences with different modifications, including glycosylation, phosphorylation and oxidation	318:432	CMP has two major amino acid sequences with different modifications, including glycosylation, phosphorylation and oxidation.
34574138	4	60	theme	previous	447:454	arg1	work					456:459	no previous work	444:459	no previous work	444:459	However, no previous work has provided a comprehensive profile of intact CMP.
34574138	9	61	theme	future	1397:1402	arg1	studies					1404:1410	future studies	1397:1410	future studies of product quality, digestive survival and bioactivity	1397:1465	Overall, this novel approach provides comprehensive characterization of CMP and CMP-derived peptides and glycopeptides, and it can be applied in future studies of product quality, digestive survival and bioactivity.
34574138	8	62	theme	protein	1234:1240	arg1	isolate					1242:1248	one whey protein isolate	1225:1248	one whey protein isolate	1225:1248	Fifty-one intact CMPs and 159 CMP-derived peptides were identified in four samples (one CMP standard, two commercial CMP products and one whey protein isolate).
34574138	2	63	theme	anti-inflammatory	262:278	arg1	activities					298:307	in vitro anti-inflammatory and antibacterial activities	253:307	in vitro anti-inflammatory and antibacterial activities	253:307	CMP has in vitro anti-inflammatory and antibacterial activities.
34574138	9	64	theme	comprehensive	1290:1302	arg1	characterization					1304:1319	comprehensive characterization	1290:1319	comprehensive characterization of CMP and CMP-derived peptides	1290:1351	Overall, this novel approach provides comprehensive characterization of CMP and CMP-derived peptides and glycopeptides, and it can be applied in future studies of product quality, digestive survival and bioactivity.
34574138	1	65	theme	bovine	144:149	arg1	kappa-casein					151:162	bovine kappa-casein	144:162	bovine kappa-casein	144:162	Caseinomacropeptide (CMP) is released from bovine kappa-casein after rennet treatment and is one of the major peptides in whey protein isolate.
34574138	7	66	theme	other	1019:1023	arg1	modifications					1044:1056	other post-translational modifications	1019:1056	other post-translational modifications	1019:1056	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra of CMPs were annotated to confirm peptide sequence, glycan composition and other post-translational modifications using automatic data processing.
34574138	1	67	theme	protein	228:234	arg1	isolate					236:242	whey protein isolate	223:242	whey protein isolate	223:242	Caseinomacropeptide (CMP) is released from bovine kappa-casein after rennet treatment and is one of the major peptides in whey protein isolate.
34574138	8	68	theme	whey	1229:1232	arg1	isolate					1242:1248	one whey protein isolate	1225:1248	one whey protein isolate	1225:1248	Fifty-one intact CMPs and 159 CMP-derived peptides were identified in four samples (one CMP standard, two commercial CMP products and one whey protein isolate).
34574138	6	69	theme	nano-liquid	786:796	arg1	chromatography					798:811	nano-liquid chromatography	786:811	nano-liquid chromatography with electron-transfer/higher-energy collision dissociation	786:871	In this study, we developed a top-down glycopeptidomics-based analytical method to profile CMP and CMP-derived peptides using Orbitrap mass spectrometry combined with nano-liquid chromatography with electron-transfer/higher-energy collision dissociation.
34574138	6	70	theme	analytical	681:690	arg1	method					692:697	a top-down glycopeptidomics-based analytical method	647:697	a top-down glycopeptidomics-based analytical method to profile CMP and CMP-derived peptides	647:737	In this study, we developed a top-down glycopeptidomics-based analytical method to profile CMP and CMP-derived peptides using Orbitrap mass spectrometry combined with nano-liquid chromatography with electron-transfer/higher-energy collision dissociation.
34574138	2	71	theme	in	253:254	arg1	activities					298:307	in vitro anti-inflammatory and antibacterial activities	253:307	in vitro anti-inflammatory and antibacterial activities	253:307	CMP has in vitro anti-inflammatory and antibacterial activities.
34574138	6	72	theme	top-down	649:656	arg1	method					692:697	a top-down glycopeptidomics-based analytical method	647:697	a top-down glycopeptidomics-based analytical method to profile CMP and CMP-derived peptides	647:737	In this study, we developed a top-down glycopeptidomics-based analytical method to profile CMP and CMP-derived peptides using Orbitrap mass spectrometry combined with nano-liquid chromatography with electron-transfer/higher-energy collision dissociation.
34574138	6	73	theme	glycopeptidomics-based	658:679	arg1	method					692:697	a top-down glycopeptidomics-based analytical method	647:697	a top-down glycopeptidomics-based analytical method to profile CMP and CMP-derived peptides	647:737	In this study, we developed a top-down glycopeptidomics-based analytical method to profile CMP and CMP-derived peptides using Orbitrap mass spectrometry combined with nano-liquid chromatography with electron-transfer/higher-energy collision dissociation.
34574138	0	74	theme	Mass	82:85	arg1	Spectrometry					87:98	Liquid Chromatography-Tandem Mass Spectrometry	53:98	Liquid Chromatography-Tandem Mass Spectrometry	53:98	Analysis of Bovine Kappa-Casein Glycomacropeptide by Liquid Chromatography-Tandem Mass Spectrometry.
34574138	9	75	theme	novel	1266:1270	arg1	approach					1272:1279	this novel approach	1261:1279	this novel approach	1261:1279	Overall, this novel approach provides comprehensive characterization of CMP and CMP-derived peptides and glycopeptides, and it can be applied in future studies of product quality, digestive survival and bioactivity.
34574138	1	76	theme	rennet	170:175	arg1	treatment					177:185	rennet treatment	170:185	rennet treatment	170:185	Caseinomacropeptide (CMP) is released from bovine kappa-casein after rennet treatment and is one of the major peptides in whey protein isolate.
34574138	6	77	theme	mass	754:757	arg1	spectrometry					759:770	Orbitrap mass spectrometry	745:770	Orbitrap mass spectrometry combined with nano-liquid chromatography with electron-transfer/higher-energy collision dissociation	745:871	In this study, we developed a top-down glycopeptidomics-based analytical method to profile CMP and CMP-derived peptides using Orbitrap mass spectrometry combined with nano-liquid chromatography with electron-transfer/higher-energy collision dissociation.
34574138	7	78	theme	post-translational	1025:1042	arg1	modifications					1044:1056	other post-translational modifications	1019:1056	other post-translational modifications	1019:1056	The liquid chromatography-tandem mass spectrometry (LC-MS/MS) spectra of CMPs were annotated to confirm peptide sequence, glycan composition and other post-translational modifications using automatic data processing.
34438010	7	0	theme	fermentable	1097:1107	arg1	fibers					1109:1114	partly fermentable fibers	1090:1114	partly fermentable fibers	1090:1114	CONCLUSION LA-AG was partly fermentable fibers with prebiotic potential for human gut health.
34438010	1	1	dep	BACKGROUND	105:114	arg1	fibers					139:144	Dietary fibers	131:144	Dietary fibers	131:144	BACKGROUND AND OBJECTIVES Dietary fibers have beneficial effects on human health through the interaction with gut microbiota.
34438010	1	2	contain	have	146:149	arg1	OBJECTIVES					120:129	OBJECTIVES	120:129	OBJECTIVES	120:129	BACKGROUND AND OBJECTIVES Dietary fibers have beneficial effects on human health through the interaction with gut microbiota.
34438010	1	2	contain	have	146:149	arg2	effects					162:168	beneficial effects	151:168	beneficial effects on human health	151:184	BACKGROUND AND OBJECTIVES Dietary fibers have beneficial effects on human health through the interaction with gut microbiota.
34438010	1	2	contain	have	146:149	arg1	BACKGROUND					105:114	BACKGROUND	105:114	BACKGROUND	105:114	BACKGROUND AND OBJECTIVES Dietary fibers have beneficial effects on human health through the interaction with gut microbiota.
34438010	7	3	with	fibers	1109:1114	arg1	potential					1131:1139	prebiotic potential	1121:1139	prebiotic potential for human gut health	1121:1160	CONCLUSION LA-AG was partly fermentable fibers with prebiotic potential for human gut health.
34438010	4	4	theme	lactic	747:752	arg1	acids					713:717	the organic acids	701:717	the organic acids (especially acetic acid and lactic acid) accumulating	701:771	During the fermentation, pH decreased continuously, along with the organic acids (especially acetic acid and lactic acid) accumulating.
34438010	4	4	theme	lactic	747:752	arg1	acid					754:757	lactic acid	747:757	lactic acid	747:757	During the fermentation, pH decreased continuously, along with the organic acids (especially acetic acid and lactic acid) accumulating.
34438010	2	5	theme	fibers	305:310	arg1	kind					273:276	one kind	269:276	one kind of complex soluble dietary fibers that may be utilized by human gut microbiota	269:355	Larch wood arabinogalactan (LA-AG) is one kind of complex soluble dietary fibers that may be utilized by human gut microbiota.
34438010	3	6	theme	fatty	548:552	arg1	acid					576:579	lactic acid	569:579	lactic acid	569:579	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	3	6	theme	fatty	548:552	arg1	acid					591:594	succinic acid	582:594	succinic acid	582:594	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	3	6	theme	fatty	548:552	arg1	SCFAs					561:565	SCFAs	561:565	SCFAs	561:565	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	3	6	theme	fatty	548:552	arg1	acids					554:558	short-chain fatty acids	536:558	short-chain fatty acids (SCFAs)	536:566	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	2	7	theme	dietary	297:303	arg1	fibers					305:310	complex soluble dietary fibers	281:310	complex soluble dietary fibers that may be utilized by human gut microbiota	281:355	Larch wood arabinogalactan (LA-AG) is one kind of complex soluble dietary fibers that may be utilized by human gut microbiota.
34438010	7	8	theme	CONCLUSION	1069:1078	arg1	LA-AG					1080:1084	CONCLUSION LA-AG	1069:1084	CONCLUSION LA-AG	1069:1084	CONCLUSION LA-AG was partly fermentable fibers with prebiotic potential for human gut health.
34438010	2	9	theme	soluble	289:295	arg1	fibers					305:310	complex soluble dietary fibers	281:310	complex soluble dietary fibers that may be utilized by human gut microbiota	281:355	Larch wood arabinogalactan (LA-AG) is one kind of complex soluble dietary fibers that may be utilized by human gut microbiota.
34438010	6	10	theme	gut	1053:1055	arg1	microbiota					1057:1066	gut microbiota	1053:1066	gut microbiota	1053:1066	In addition, LA-AG inhibited the proliferation of some gut microbiota (Unclassified_Enterobacteriaceae and Citrobacter) and the accumulation of some metabolites (Sulfide and indole) released by gut microbiota.
34438010	4	11	dep	acids	713:717	arg1	acids					713:717	the organic acids	701:717	the organic acids (especially acetic acid and lactic acid) accumulating	701:771	During the fermentation, pH decreased continuously, along with the organic acids (especially acetic acid and lactic acid) accumulating.
34438010	4	11	dep	acids	713:717	arg1	acid					738:741	acetic acid	731:741	acetic acid	731:741	During the fermentation, pH decreased continuously, along with the organic acids (especially acetic acid and lactic acid) accumulating.
34438010	4	11	dep	acids	713:717	arg1	acid					754:757	lactic acid	747:757	lactic acid	747:757	During the fermentation, pH decreased continuously, along with the organic acids (especially acetic acid and lactic acid) accumulating.
34438010	6	12	dep	metabolites	1008:1018	arg1	indole					1033:1038	indole	1033:1038	indole	1033:1038	In addition, LA-AG inhibited the proliferation of some gut microbiota (Unclassified_Enterobacteriaceae and Citrobacter) and the accumulation of some metabolites (Sulfide and indole) released by gut microbiota.
34438010	6	12	dep	metabolites	1008:1018	arg1	Sulfide					1021:1027	Sulfide	1021:1027	Sulfide	1021:1027	In addition, LA-AG inhibited the proliferation of some gut microbiota (Unclassified_Enterobacteriaceae and Citrobacter) and the accumulation of some metabolites (Sulfide and indole) released by gut microbiota.
34438010	6	12	dep	metabolites	1008:1018	arg1	metabolites					1008:1018	some metabolites	1003:1018	some metabolites (Sulfide and indole) released by gut microbiota	1003:1066	In addition, LA-AG inhibited the proliferation of some gut microbiota (Unclassified_Enterobacteriaceae and Citrobacter) and the accumulation of some metabolites (Sulfide and indole) released by gut microbiota.
34438010	2	13	theme	complex	281:287	arg1	fibers					305:310	complex soluble dietary fibers	281:310	complex soluble dietary fibers that may be utilized by human gut microbiota	281:355	Larch wood arabinogalactan (LA-AG) is one kind of complex soluble dietary fibers that may be utilized by human gut microbiota.
34438010	5	14	theme	beneficial	821:830	arg1	metabolites					832:842	some beneficial metabolites	816:842	some beneficial metabolites	816:842	LA-AG was degraded by gut microbiota then some beneficial metabolites were produced.
34438010	6	15	theme	microbiota	918:927	arg1	proliferation					892:904	the proliferation	888:904	the proliferation of some gut microbiota (Unclassified_Enterobacteriaceae and Citrobacter)	888:977	In addition, LA-AG inhibited the proliferation of some gut microbiota (Unclassified_Enterobacteriaceae and Citrobacter) and the accumulation of some metabolites (Sulfide and indole) released by gut microbiota.
34438010	6	15	theme	microbiota	918:927	arg1	accumulation					987:998	the accumulation	983:998	the accumulation of some metabolites (Sulfide and indole) released by gut microbiota	983:1066	In addition, LA-AG inhibited the proliferation of some gut microbiota (Unclassified_Enterobacteriaceae and Citrobacter) and the accumulation of some metabolites (Sulfide and indole) released by gut microbiota.
34438010	1	16	from	effects	162:168	arg1	health					179:184	human health	173:184	human health	173:184	BACKGROUND AND OBJECTIVES Dietary fibers have beneficial effects on human health through the interaction with gut microbiota.
34438010	4	17	theme	organic	705:711	arg1	acids					713:717	the organic acids	701:717	the organic acids (especially acetic acid and lactic acid) accumulating	701:771	During the fermentation, pH decreased continuously, along with the organic acids (especially acetic acid and lactic acid) accumulating.
34438010	4	17	theme	organic	705:711	arg1	acid					738:741	acetic acid	731:741	acetic acid	731:741	During the fermentation, pH decreased continuously, along with the organic acids (especially acetic acid and lactic acid) accumulating.
34438010	4	17	theme	organic	705:711	arg1	acid					754:757	lactic acid	747:757	lactic acid	747:757	During the fermentation, pH decreased continuously, along with the organic acids (especially acetic acid and lactic acid) accumulating.
34438010	3	18	theme	gut	418:420	arg1	microbiota					422:431	gut microbiota	418:431	gut microbiota	418:431	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	0	19	theme	Prebiotic	0:8	arg1	characteristics					10:24	Prebiotic characteristics	0:24	Prebiotic characteristics of arabinogalactans	0:44	Prebiotic characteristics of arabinogalactans during in vitro fermentation through multi-omics analysis.
34438010	4	20	theme	acetic	731:736	arg1	acids					713:717	the organic acids	701:717	the organic acids (especially acetic acid and lactic acid) accumulating	701:771	During the fermentation, pH decreased continuously, along with the organic acids (especially acetic acid and lactic acid) accumulating.
34438010	4	20	theme	acetic	731:736	arg1	acid					738:741	acetic acid	731:741	acetic acid	731:741	During the fermentation, pH decreased continuously, along with the organic acids (especially acetic acid and lactic acid) accumulating.
34438010	1	21	theme	gut	215:217	arg1	microbiota					219:228	gut microbiota	215:228	gut microbiota	215:228	BACKGROUND AND OBJECTIVES Dietary fibers have beneficial effects on human health through the interaction with gut microbiota.
34438010	0	22	theme	arabinogalactans	29:44	arg1	characteristics					10:24	Prebiotic characteristics	0:24	Prebiotic characteristics of arabinogalactans	0:44	Prebiotic characteristics of arabinogalactans during in vitro fermentation through multi-omics analysis.
34438010	3	23	theme	succinic	582:589	arg1	acid					591:594	succinic acid	582:594	succinic acid	582:594	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	3	23	theme	succinic	582:589	arg1	acids					554:558	short-chain fatty acids	536:558	short-chain fatty acids (SCFAs)	536:566	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	3	24	theme	acids	554:558	arg1	metabolites					625:635	volatile organic metabolites	608:635	volatile organic metabolites	608:635	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	3	24	theme	acids	554:558	arg1	production					522:531	the production	518:531	the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid	518:594	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	3	24	theme	acids	554:558	arg1	LA-AG					483:487	LA-AG	483:487	LA-AG	483:487	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	3	24	theme	acids	554:558	arg1	composition					501:511	microbiota composition	490:511	microbiota composition	490:511	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	6	25	dep	microbiota	918:927	arg1	Citrobacter					966:976	Citrobacter	966:976	Citrobacter	966:976	In addition, LA-AG inhibited the proliferation of some gut microbiota (Unclassified_Enterobacteriaceae and Citrobacter) and the accumulation of some metabolites (Sulfide and indole) released by gut microbiota.
34438010	6	25	dep	microbiota	918:927	arg1	Unclassified_Enterobacteriaceae					930:960	Unclassified_Enterobacteriaceae	930:960	Unclassified_Enterobacteriaceae	930:960	In addition, LA-AG inhibited the proliferation of some gut microbiota (Unclassified_Enterobacteriaceae and Citrobacter) and the accumulation of some metabolites (Sulfide and indole) released by gut microbiota.
34438010	3	26	theme	production	522:531	arg1	change					473:478	the change	469:478	the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites	469:635	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	3	27	theme	volatile	608:615	arg1	metabolites					625:635	volatile organic metabolites	608:635	volatile organic metabolites	608:635	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	1	28	theme	Dietary	131:137	arg1	fibers					139:144	Dietary fibers	131:144	Dietary fibers	131:144	BACKGROUND AND OBJECTIVES Dietary fibers have beneficial effects on human health through the interaction with gut microbiota.
34438010	5	29	theme	gut	796:798	arg1	microbiota					800:809	gut microbiota	796:809	gut microbiota	796:809	LA-AG was degraded by gut microbiota then some beneficial metabolites were produced.
34438010	0	30	theme	in	53:54	arg1	fermentation					62:73	in vitro fermentation	53:73	in vitro fermentation through multi-omics analysis	53:102	Prebiotic characteristics of arabinogalactans during in vitro fermentation through multi-omics analysis.
34438010	3	31	theme	microbiota	490:499	arg1	composition					501:511	microbiota composition	490:511	microbiota composition	490:511	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	3	32	theme	organic	617:623	arg1	metabolites					625:635	volatile organic metabolites	608:635	volatile organic metabolites	608:635	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	6	33	theme	gut	914:916	arg1	microbiota					918:927	some gut microbiota	909:927	some gut microbiota (Unclassified_Enterobacteriaceae and Citrobacter)	909:977	In addition, LA-AG inhibited the proliferation of some gut microbiota (Unclassified_Enterobacteriaceae and Citrobacter) and the accumulation of some metabolites (Sulfide and indole) released by gut microbiota.
34438010	7	34	theme	human	1145:1149	arg1	health					1155:1160	human gut health	1145:1160	human gut health	1145:1160	CONCLUSION LA-AG was partly fermentable fibers with prebiotic potential for human gut health.
34438010	3	35	theme	composition	501:511	arg1	change					473:478	the change	469:478	the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites	469:635	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	0	36	dep	in	53:54	arg1	vitro					56:60	vitro	56:60	vitro	56:60	Prebiotic characteristics of arabinogalactans during in vitro fermentation through multi-omics analysis.
34438010	3	37	theme	LA-AG	397:401	arg1	degradation					403:413	the LA-AG degradation	393:413	the LA-AG degradation by gut microbiota	393:431	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	3	38	dep	METHODS	358:364	arg1	characterized					438:450	characterized	438:450	were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites	433:635	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	2	39	theme	wood	237:240	arg1	LA-AG					259:263	LA-AG	259:263	LA-AG	259:263	Larch wood arabinogalactan (LA-AG) is one kind of complex soluble dietary fibers that may be utilized by human gut microbiota.
34438010	2	39	theme	wood	237:240	arg1	arabinogalactan					242:256	Larch wood arabinogalactan	231:256	Larch wood arabinogalactan (LA-AG)	231:264	Larch wood arabinogalactan (LA-AG) is one kind of complex soluble dietary fibers that may be utilized by human gut microbiota.
34438010	3	40	theme	lactic	569:574	arg1	acid					576:579	lactic acid	569:579	lactic acid	569:579	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	3	40	theme	lactic	569:574	arg1	acids					554:558	short-chain fatty acids	536:558	short-chain fatty acids (SCFAs)	536:566	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	2	41	theme	gut	342:344	arg1	microbiota					346:355	human gut microbiota	336:355	human gut microbiota	336:355	Larch wood arabinogalactan (LA-AG) is one kind of complex soluble dietary fibers that may be utilized by human gut microbiota.
34438010	2	42	theme	Larch	231:235	arg1	LA-AG					259:263	LA-AG	259:263	LA-AG	259:263	Larch wood arabinogalactan (LA-AG) is one kind of complex soluble dietary fibers that may be utilized by human gut microbiota.
34438010	2	42	theme	Larch	231:235	arg1	arabinogalactan					242:256	Larch wood arabinogalactan	231:256	Larch wood arabinogalactan (LA-AG)	231:264	Larch wood arabinogalactan (LA-AG) is one kind of complex soluble dietary fibers that may be utilized by human gut microbiota.
34438010	7	43	theme	prebiotic	1121:1129	arg1	potential					1131:1139	prebiotic potential	1121:1139	prebiotic potential for human gut health	1121:1160	CONCLUSION LA-AG was partly fermentable fibers with prebiotic potential for human gut health.
34438010	1	44	theme	beneficial	151:160	arg1	effects					162:168	beneficial effects	151:168	beneficial effects on human health	151:184	BACKGROUND AND OBJECTIVES Dietary fibers have beneficial effects on human health through the interaction with gut microbiota.
34438010	6	45	theme	metabolites	1008:1018	arg1	proliferation					892:904	the proliferation	888:904	the proliferation of some gut microbiota (Unclassified_Enterobacteriaceae and Citrobacter)	888:977	In addition, LA-AG inhibited the proliferation of some gut microbiota (Unclassified_Enterobacteriaceae and Citrobacter) and the accumulation of some metabolites (Sulfide and indole) released by gut microbiota.
34438010	6	45	theme	metabolites	1008:1018	arg1	accumulation					987:998	the accumulation	983:998	the accumulation of some metabolites (Sulfide and indole) released by gut microbiota	983:1066	In addition, LA-AG inhibited the proliferation of some gut microbiota (Unclassified_Enterobacteriaceae and Citrobacter) and the accumulation of some metabolites (Sulfide and indole) released by gut microbiota.
34438010	1	46	with	interaction	198:208	arg1	microbiota					219:228	gut microbiota	215:228	gut microbiota	215:228	BACKGROUND AND OBJECTIVES Dietary fibers have beneficial effects on human health through the interaction with gut microbiota.
34438010	3	47	theme	metabolites	625:635	arg1	change					473:478	the change	469:478	the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites	469:635	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	3	48	theme	short-chain	536:546	arg1	acid					576:579	lactic acid	569:579	lactic acid	569:579	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	3	48	theme	short-chain	536:546	arg1	acid					591:594	succinic acid	582:594	succinic acid	582:594	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	3	48	theme	short-chain	536:546	arg1	SCFAs					561:565	SCFAs	561:565	SCFAs	561:565	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	3	48	theme	short-chain	536:546	arg1	acids					554:558	short-chain fatty acids	536:558	short-chain fatty acids (SCFAs)	536:566	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	3	49	theme	LA-AG	483:487	arg1	change					473:478	the change	469:478	the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites	469:635	METHODS AND RESULTS In this study, the LA-AG degradation by gut microbiota were characterized by investigating the change of LA-AG, microbiota composition, and the production of short-chain fatty acids (SCFAs), lactic acid, succinic acid, as well as volatile organic metabolites.
34438010	2	50	theme	human	336:340	arg1	microbiota					346:355	human gut microbiota	336:355	human gut microbiota	336:355	Larch wood arabinogalactan (LA-AG) is one kind of complex soluble dietary fibers that may be utilized by human gut microbiota.
34438010	0	51	theme	multi-omics	83:93	arg1	analysis					95:102	multi-omics analysis	83:102	multi-omics analysis	83:102	Prebiotic characteristics of arabinogalactans during in vitro fermentation through multi-omics analysis.
34438010	0	52	dep	characteristics	10:24	arg1	fermentation					62:73	in vitro fermentation	53:73	in vitro fermentation through multi-omics analysis	53:102	Prebiotic characteristics of arabinogalactans during in vitro fermentation through multi-omics analysis.
34438010	2	53	used	utilized	324:331	arg2	fibers					305:310	complex soluble dietary fibers	281:310	complex soluble dietary fibers that may be utilized by human gut microbiota	281:355	Larch wood arabinogalactan (LA-AG) is one kind of complex soluble dietary fibers that may be utilized by human gut microbiota.
34438010	7	54	theme	gut	1151:1153	arg1	health					1155:1160	human gut health	1145:1160	human gut health	1145:1160	CONCLUSION LA-AG was partly fermentable fibers with prebiotic potential for human gut health.
34438010	1	55	theme	human	173:177	arg1	health					179:184	human health	173:184	human health	173:184	BACKGROUND AND OBJECTIVES Dietary fibers have beneficial effects on human health through the interaction with gut microbiota.
33722619	0	0	theme	cellulose	71:79	arg1	fibers					81:86	cellulose fibers	71:86	cellulose fibers of luffa sponge	71:102	Influence of chemical treatment and drying method on the properties of cellulose fibers of luffa sponge.
33722619	4	1	theme	fiber	722:726	arg1	density					728:734	their fiber density	716:734	their fiber density	716:734	Meanwhile, freeze-drying kept the cells of modified LS fibers full and decreased their fiber density by 5.0% and 21.0%, respectively.
33722619	1	2	theme	sponge	139:144	arg1	fiber					161:165	modified luffa sponge (LS) cellulose fiber	124:165	modified luffa sponge (LS) cellulose fiber	124:165	The exploration of modified luffa sponge (LS) cellulose fiber in the field of polymer composite can contribute to the development of high-performance and lightweight composites.
33722619	7	3	theme	fibers	1315:1320	arg1	temperatures					1286:1297	onset degradation temperatures	1268:1297	onset degradation temperatures of the modified fibers	1268:1320	Moreover, the thermogravimetric analysis reveals that freeze-drying increased onset degradation temperatures of the modified fibers by 24.0 °C and 6.7 °C, which was beneficial to improve the thermal stability of the composite material.
33722619	7	4	theme	composite	1406:1414	arg1	material					1416:1423	the composite material	1402:1423	the composite material	1402:1423	Moreover, the thermogravimetric analysis reveals that freeze-drying increased onset degradation temperatures of the modified fibers by 24.0 °C and 6.7 °C, which was beneficial to improve the thermal stability of the composite material.
33722619	6	5	theme	absorption	953:962	arg1	analyses					964:971	The moisture absorption analyses	940:971	The moisture absorption analyses	940:971	The moisture absorption analyses showed that freeze-drying could further decrease the moisture absorption ratios of modified LS fibers by 25.8% and 35.8%, respectively, which was useful for improving the dimensional stability of composite materials.
33722619	7	6	theme	material	1416:1423	arg1	stability					1389:1397	the thermal stability	1377:1397	the thermal stability of the composite material	1377:1423	Moreover, the thermogravimetric analysis reveals that freeze-drying increased onset degradation temperatures of the modified fibers by 24.0 °C and 6.7 °C, which was beneficial to improve the thermal stability of the composite material.
33722619	0	7	from	Influence	0:8	arg1	properties					57:66	the properties	53:66	the properties of cellulose fibers of luffa sponge	53:102	Influence of chemical treatment and drying method on the properties of cellulose fibers of luffa sponge.
33722619	6	8	theme	fibers	1068:1073	arg1	ratios					1046:1051	the moisture absorption ratios	1022:1051	the moisture absorption ratios of modified LS fibers	1022:1073	The moisture absorption analyses showed that freeze-drying could further decrease the moisture absorption ratios of modified LS fibers by 25.8% and 35.8%, respectively, which was useful for improving the dimensional stability of composite materials.
33722619	6	9	theme	moisture	1026:1033	arg1	ratios					1046:1051	the moisture absorption ratios	1022:1051	the moisture absorption ratios of modified LS fibers	1022:1073	The moisture absorption analyses showed that freeze-drying could further decrease the moisture absorption ratios of modified LS fibers by 25.8% and 35.8%, respectively, which was useful for improving the dimensional stability of composite materials.
33722619	0	10	theme	fibers	81:86	arg1	properties					57:66	the properties	53:66	the properties of cellulose fibers of luffa sponge	53:102	Influence of chemical treatment and drying method on the properties of cellulose fibers of luffa sponge.
33722619	3	11	theme	fiber	601:605	arg1	density					607:613	their fiber density	595:613	their fiber density	595:613	The microscopic characteristics and physical properties showed that Methods 1 and 2 caused shrinkage of the LS fibers and increased their fiber density by 30.6% and 15.0%.
33722619	0	12	theme	sponge	97:102	arg1	fibers					81:86	cellulose fibers	71:86	cellulose fibers of luffa sponge	71:102	Influence of chemical treatment and drying method on the properties of cellulose fibers of luffa sponge.
33722619	4	13	theme	full	697:700	arg1	fibers					690:695	modified LS fibers full	678:700	modified LS fibers full	678:700	Meanwhile, freeze-drying kept the cells of modified LS fibers full and decreased their fiber density by 5.0% and 21.0%, respectively.
33722619	7	14	theme	onset	1268:1272	arg1	temperatures					1286:1297	onset degradation temperatures	1268:1297	onset degradation temperatures of the modified fibers	1268:1320	Moreover, the thermogravimetric analysis reveals that freeze-drying increased onset degradation temperatures of the modified fibers by 24.0 °C and 6.7 °C, which was beneficial to improve the thermal stability of the composite material.
33722619	1	15	theme	LS	147:148	arg1	fiber					161:165	modified luffa sponge (LS) cellulose fiber	124:165	modified luffa sponge (LS) cellulose fiber	124:165	The exploration of modified luffa sponge (LS) cellulose fiber in the field of polymer composite can contribute to the development of high-performance and lightweight composites.
33722619	0	16	theme	luffa	91:95	arg1	sponge					97:102	luffa sponge	91:102	luffa sponge	91:102	Influence of chemical treatment and drying method on the properties of cellulose fibers of luffa sponge.
33722619	4	17	theme	fibers	690:695	arg1	cells					669:673	the cells	665:673	the cells of modified LS fibers full	665:700	Meanwhile, freeze-drying kept the cells of modified LS fibers full and decreased their fiber density by 5.0% and 21.0%, respectively.
33722619	1	18	from	exploration	109:119	arg1	field					174:178	the field	170:178	the field of polymer composite	170:199	The exploration of modified luffa sponge (LS) cellulose fiber in the field of polymer composite can contribute to the development of high-performance and lightweight composites.
33722619	7	19	theme	degradation	1274:1284	arg1	temperatures					1286:1297	onset degradation temperatures	1268:1297	onset degradation temperatures of the modified fibers	1268:1320	Moreover, the thermogravimetric analysis reveals that freeze-drying increased onset degradation temperatures of the modified fibers by 24.0 °C and 6.7 °C, which was beneficial to improve the thermal stability of the composite material.
33722619	6	20	theme	materials	1179:1187	arg1	stability					1156:1164	the dimensional stability	1140:1164	the dimensional stability of composite materials	1140:1187	The moisture absorption analyses showed that freeze-drying could further decrease the moisture absorption ratios of modified LS fibers by 25.8% and 35.8%, respectively, which was useful for improving the dimensional stability of composite materials.
33722619	7	21	theme	modified	1306:1313	arg1	fibers					1315:1320	the modified fibers	1302:1320	the modified fibers	1302:1320	Moreover, the thermogravimetric analysis reveals that freeze-drying increased onset degradation temperatures of the modified fibers by 24.0 °C and 6.7 °C, which was beneficial to improve the thermal stability of the composite material.
33722619	1	22	theme	cellulose	151:159	arg1	fiber					161:165	modified luffa sponge (LS) cellulose fiber	124:165	modified luffa sponge (LS) cellulose fiber	124:165	The exploration of modified luffa sponge (LS) cellulose fiber in the field of polymer composite can contribute to the development of high-performance and lightweight composites.
33722619	2	23	theme	%	366:366	arg1	Method					375:380	Method 2	375:382	Method 2	375:382	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	2	23	theme	%	366:366	arg1	Na2SO3					367:372	10%NaOH-5%Na2SO3	357:372	10%NaOH-5%Na2SO3 (Method 2)	357:383	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	1	24	theme	high-performance	238:253	arg1	composites					271:280	high-performance and lightweight composites	238:280	high-performance and lightweight composites	238:280	The exploration of modified luffa sponge (LS) cellulose fiber in the field of polymer composite can contribute to the development of high-performance and lightweight composites.
33722619	5	25	theme	tensile	773:779	arg1	properties					781:790	The tensile properties	769:790	The tensile properties test analyses	769:804	The tensile properties test analyses indicated that freeze-drying further increased the elongation at break values of modified LS fibers by 25.3% and 17.7%, respectively.
33722619	1	26	theme	fiber	161:165	arg1	exploration					109:119	The exploration	105:119	The exploration of modified luffa sponge (LS) cellulose fiber in the field of polymer composite	105:199	The exploration of modified luffa sponge (LS) cellulose fiber in the field of polymer composite can contribute to the development of high-performance and lightweight composites.
33722619	0	27	theme	treatment	22:30	arg1	Influence					0:8	Influence	0:8	Influence of chemical treatment and drying method on the properties of cellulose fibers of luffa sponge.	0:103	Influence of chemical treatment and drying method on the properties of cellulose fibers of luffa sponge.
33722619	2	28	theme	%	325:325	arg1	%					333:333	10%NaOH-20%CH3COOH	323:340	10%NaOH-20%CH3COOH (Method 1)	323:351	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	2	28	theme	%	325:325	arg1	Method					343:348	Method 1	343:350	Method 1	343:350	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	6	29	theme	LS	1065:1066	arg1	fibers					1068:1073	modified LS fibers	1056:1073	modified LS fibers	1056:1073	The moisture absorption analyses showed that freeze-drying could further decrease the moisture absorption ratios of modified LS fibers by 25.8% and 35.8%, respectively, which was useful for improving the dimensional stability of composite materials.
33722619	2	30	dep	treatments	311:320	arg1	Method					343:348	Method 1	343:350	Method 1	343:350	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	2	30	dep	treatments	311:320	arg1	drying					414:419	air drying	410:419	air drying	410:419	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	2	30	dep	treatments	311:320	arg1	Na2SO3					367:372	10%NaOH-5%Na2SO3	357:372	10%NaOH-5%Na2SO3 (Method 2)	357:383	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	2	30	dep	treatments	311:320	arg1	freeze-drying					425:437	freeze-drying	425:437	freeze-drying	425:437	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	2	30	dep	treatments	311:320	arg1	Method					375:380	Method 2	375:382	Method 2	375:382	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	2	30	dep	treatments	311:320	arg1	%					333:333	10%NaOH-20%CH3COOH	323:340	10%NaOH-20%CH3COOH (Method 1)	323:351	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	2	30	dep	treatments	311:320	arg1	treatments					311:320	two chemical treatments	298:320	two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2))	298:384	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	7	31	theme	thermogravimetric	1204:1220	arg1	analysis					1222:1229	the thermogravimetric analysis	1200:1229	the thermogravimetric analysis	1200:1229	Moreover, the thermogravimetric analysis reveals that freeze-drying increased onset degradation temperatures of the modified fibers by 24.0 °C and 6.7 °C, which was beneficial to improve the thermal stability of the composite material.
33722619	2	32	theme	10	357:358	arg1	%					359:359	%	359:359	%	359:359	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	0	33	theme	chemical	13:20	arg1	treatment					22:30	chemical treatment	13:30	chemical treatment	13:30	Influence of chemical treatment and drying method on the properties of cellulose fibers of luffa sponge.
33722619	1	34	theme	lightweight	259:269	arg1	composites					271:280	high-performance and lightweight composites	238:280	high-performance and lightweight composites	238:280	The exploration of modified luffa sponge (LS) cellulose fiber in the field of polymer composite can contribute to the development of high-performance and lightweight composites.
33722619	7	35	theme	thermal	1381:1387	arg1	stability					1389:1397	the thermal stability	1377:1397	the thermal stability of the composite material	1377:1423	Moreover, the thermogravimetric analysis reveals that freeze-drying increased onset degradation temperatures of the modified fibers by 24.0 °C and 6.7 °C, which was beneficial to improve the thermal stability of the composite material.
33722619	5	36	theme	test	792:795	arg1	analyses					797:804	The tensile properties test analyses	769:804	The tensile properties test analyses	769:804	The tensile properties test analyses indicated that freeze-drying further increased the elongation at break values of modified LS fibers by 25.3% and 17.7%, respectively.
33722619	0	37	theme	drying	36:41	arg1	method					43:48	drying method	36:48	drying method	36:48	Influence of chemical treatment and drying method on the properties of cellulose fibers of luffa sponge.
33722619	1	38	theme	composites	271:280	arg1	development					223:233	the development	219:233	the development of high-performance and lightweight composites	219:280	The exploration of modified luffa sponge (LS) cellulose fiber in the field of polymer composite can contribute to the development of high-performance and lightweight composites.
33722619	6	39	theme	dimensional	1144:1154	arg1	stability					1156:1164	the dimensional stability	1140:1164	the dimensional stability of composite materials	1140:1187	The moisture absorption analyses showed that freeze-drying could further decrease the moisture absorption ratios of modified LS fibers by 25.8% and 35.8%, respectively, which was useful for improving the dimensional stability of composite materials.
33722619	3	40	theme	microscopic	467:477	arg1	characteristics					479:493	The microscopic characteristics	463:493	The microscopic characteristics	463:493	The microscopic characteristics and physical properties showed that Methods 1 and 2 caused shrinkage of the LS fibers and increased their fiber density by 30.6% and 15.0%.
33722619	6	41	theme	absorption	1035:1044	arg1	ratios					1046:1051	the moisture absorption ratios	1022:1051	the moisture absorption ratios of modified LS fibers	1022:1073	The moisture absorption analyses showed that freeze-drying could further decrease the moisture absorption ratios of modified LS fibers by 25.8% and 35.8%, respectively, which was useful for improving the dimensional stability of composite materials.
33722619	2	42	theme	NaOH-5	360:365	arg1	Method					375:380	Method 2	375:382	Method 2	375:382	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	2	42	theme	NaOH-5	360:365	arg1	Na2SO3					367:372	10%NaOH-5%Na2SO3	357:372	10%NaOH-5%Na2SO3 (Method 2)	357:383	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	6	43	theme	composite	1169:1177	arg1	materials					1179:1187	composite materials	1169:1187	composite materials	1169:1187	The moisture absorption analyses showed that freeze-drying could further decrease the moisture absorption ratios of modified LS fibers by 25.8% and 35.8%, respectively, which was useful for improving the dimensional stability of composite materials.
33722619	7	44	dep	increased	1258:1266	arg1	beneficial					1355:1364	beneficial	1355:1364	beneficial	1355:1364	Moreover, the thermogravimetric analysis reveals that freeze-drying increased onset degradation temperatures of the modified fibers by 24.0 °C and 6.7 °C, which was beneficial to improve the thermal stability of the composite material.
33722619	4	45	theme	LS	687:688	arg1	fibers					690:695	modified LS fibers full	678:700	modified LS fibers full	678:700	Meanwhile, freeze-drying kept the cells of modified LS fibers full and decreased their fiber density by 5.0% and 21.0%, respectively.
33722619	0	46	theme	method	43:48	arg1	Influence					0:8	Influence	0:8	Influence of chemical treatment and drying method on the properties of cellulose fibers of luffa sponge.	0:103	Influence of chemical treatment and drying method on the properties of cellulose fibers of luffa sponge.
33722619	5	47	theme	modified	887:894	arg1	fibers					899:904	modified LS fibers	887:904	modified LS fibers	887:904	The tensile properties test analyses indicated that freeze-drying further increased the elongation at break values of modified LS fibers by 25.3% and 17.7%, respectively.
33722619	4	48	theme	modified	678:685	arg1	fibers					690:695	modified LS fibers full	678:700	modified LS fibers full	678:700	Meanwhile, freeze-drying kept the cells of modified LS fibers full and decreased their fiber density by 5.0% and 21.0%, respectively.
33722619	2	49	theme	chemical	302:309	arg1	treatments					311:320	two chemical treatments	298:320	two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2))	298:384	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	2	49	theme	chemical	302:309	arg1	freeze-drying					425:437	freeze-drying	425:437	freeze-drying	425:437	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	2	49	theme	chemical	302:309	arg1	drying					414:419	air drying	410:419	air drying	410:419	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	3	50	theme	physical	499:506	arg1	properties					508:517	physical properties	499:517	physical properties	499:517	The microscopic characteristics and physical properties showed that Methods 1 and 2 caused shrinkage of the LS fibers and increased their fiber density by 30.6% and 15.0%.
33722619	5	51	theme	LS	896:897	arg1	fibers					899:904	modified LS fibers	887:904	modified LS fibers	887:904	The tensile properties test analyses indicated that freeze-drying further increased the elongation at break values of modified LS fibers by 25.3% and 17.7%, respectively.
33722619	6	52	theme	modified	1056:1063	arg1	fibers					1068:1073	modified LS fibers	1056:1073	modified LS fibers	1056:1073	The moisture absorption analyses showed that freeze-drying could further decrease the moisture absorption ratios of modified LS fibers by 25.8% and 35.8%, respectively, which was useful for improving the dimensional stability of composite materials.
33722619	5	53	theme	break	871:875	arg1	values					877:882	break values	871:882	break values of modified LS fibers	871:904	The tensile properties test analyses indicated that freeze-drying further increased the elongation at break values of modified LS fibers by 25.3% and 17.7%, respectively.
33722619	6	54	theme	moisture	944:951	arg1	analyses					964:971	The moisture absorption analyses	940:971	The moisture absorption analyses	940:971	The moisture absorption analyses showed that freeze-drying could further decrease the moisture absorption ratios of modified LS fibers by 25.8% and 35.8%, respectively, which was useful for improving the dimensional stability of composite materials.
33722619	5	55	theme	properties	781:790	arg1	analyses					797:804	The tensile properties test analyses	769:804	The tensile properties test analyses	769:804	The tensile properties test analyses indicated that freeze-drying further increased the elongation at break values of modified LS fibers by 25.3% and 17.7%, respectively.
33722619	1	56	theme	polymer	183:189	arg1	composite					191:199	polymer composite	183:199	polymer composite	183:199	The exploration of modified luffa sponge (LS) cellulose fiber in the field of polymer composite can contribute to the development of high-performance and lightweight composites.
33722619	5	57	theme	fibers	899:904	arg1	values					877:882	break values	871:882	break values of modified LS fibers	871:904	The tensile properties test analyses indicated that freeze-drying further increased the elongation at break values of modified LS fibers by 25.3% and 17.7%, respectively.
33722619	1	58	theme	luffa	133:137	arg1	fiber					161:165	modified luffa sponge (LS) cellulose fiber	124:165	modified luffa sponge (LS) cellulose fiber	124:165	The exploration of modified luffa sponge (LS) cellulose fiber in the field of polymer composite can contribute to the development of high-performance and lightweight composites.
33722619	1	59	theme	composite	191:199	arg1	field					174:178	the field	170:178	the field of polymer composite	170:199	The exploration of modified luffa sponge (LS) cellulose fiber in the field of polymer composite can contribute to the development of high-performance and lightweight composites.
33722619	2	60	theme	drying	394:399	arg1	methods					401:407	two drying methods	390:407	two drying methods	390:407	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	3	61	theme	LS	571:572	arg1	fibers					574:579	the LS fibers	567:579	the LS fibers	567:579	The microscopic characteristics and physical properties showed that Methods 1 and 2 caused shrinkage of the LS fibers and increased their fiber density by 30.6% and 15.0%.
33722619	6	62	dep	decrease	1013:1020	arg1	useful					1119:1124	useful	1119:1124	useful	1119:1124	The moisture absorption analyses showed that freeze-drying could further decrease the moisture absorption ratios of modified LS fibers by 25.8% and 35.8%, respectively, which was useful for improving the dimensional stability of composite materials.
33722619	1	63	theme	modified	124:131	arg1	fiber					161:165	modified luffa sponge (LS) cellulose fiber	124:165	modified luffa sponge (LS) cellulose fiber	124:165	The exploration of modified luffa sponge (LS) cellulose fiber in the field of polymer composite can contribute to the development of high-performance and lightweight composites.
33722619	3	64	theme	fibers	574:579	arg1	shrinkage					554:562	shrinkage	554:562	shrinkage of the LS fibers	554:579	The microscopic characteristics and physical properties showed that Methods 1 and 2 caused shrinkage of the LS fibers and increased their fiber density by 30.6% and 15.0%.
33722619	2	65	theme	%	359:359	arg1	Method					375:380	Method 2	375:382	Method 2	375:382	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	2	65	theme	%	359:359	arg1	Na2SO3					367:372	10%NaOH-5%Na2SO3	357:372	10%NaOH-5%Na2SO3 (Method 2)	357:383	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	2	66	used	used	445:448	arg2	drying					414:419	air drying	410:419	air drying	410:419	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	2	66	used	used	445:448	arg2	treatments					311:320	two chemical treatments	298:320	two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2))	298:384	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	2	66	used	used	445:448	arg2	methods					401:407	two drying methods	390:407	two drying methods	390:407	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	2	66	used	used	445:448	arg2	freeze-drying					425:437	freeze-drying	425:437	freeze-drying	425:437	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	2	67	theme	air	410:412	arg1	treatments					311:320	two chemical treatments	298:320	two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2))	298:384	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
33722619	2	67	theme	air	410:412	arg1	drying					414:419	air drying	410:419	air drying	410:419	In this study, two chemical treatments (10%NaOH-20%CH3COOH (Method 1) and 10%NaOH-5%Na2SO3 (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS.
31969608	7	0	theme	pH	1545:1546	arg1	values					1548:1553	different solution pH values	1526:1553	different solution pH values	1526:1553	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	10	1	theme	other	2136:2140	arg1	models					2142:2147	other models	2136:2147	other models	2136:2147	The Korsmeyer-Peppas model is most suitable for the lovastatin release process from the alginate/chitosan/lovastatin nanoparticles in the first stage, and then the drug release complies with other models depending on solution pH in the slow release stage.
31969608	6	2	theme	larger	1273:1278	arg1	degree					1288:1293	a larger crystal degree	1271:1293	a larger crystal degree	1271:1293	This result means that the alginate and chitosan interact together, so that the nanoparticles have a larger crystal degree when compared with alginate and chitosan individually.
31969608	1	3	theme	gelation	261:268	arg1	method					270:275	the ionic gelation method	251:275	the ionic gelation method	251:275	In this study, chitosan and alginate were selected to prepare alginate/chitosan nanoparticles to load the drug lovastatin by the ionic gelation method.
31969608	7	4	theme	different	1526:1534	arg1	values					1548:1553	different solution pH values	1526:1553	different solution pH values	1526:1553	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	4	5	theme	SEM	857:859	arg1	images					861:866	The laser scattering results and SEM images	824:866	The laser scattering results and SEM images	824:866	The laser scattering results and SEM images indicated that the alginate/chitosan/lovastatin nanoparticles have a spherical shape with a particle size in the range of 50-80 nm.
31969608	13	6	from	safe	2661:2664	arg1	mice					2669:2672	mice	2669:2672	mice	2669:2672	The results show that the ACL nanoparticles are safe in mice and that these composite nanoparticles might be useful as a new drug carrier.
31969608	9	7	dep	stages	1856:1861	arg1	stage					1872:1876	a rapid stage	1864:1876	two stages: a rapid stage over the first 10 hr	1852:1897	The drug release process is divided into two stages: a rapid stage over the first 10 hr, then the release becomes gradual and stable.
31969608	12	8	theme	nanoparticles	2482:2494	arg1	treatments					2440:2449	treatments	2440:2449	treatments of 100 mg/kg and 300 mg/kg ACL nanoparticles	2440:2494	The LD50 of the drug was higher than 5000 mg/kg, while in the subchronic toxicity test with treatments of 100 mg/kg and 300 mg/kg ACL nanoparticles, there were no abnormal signs, mortality, or toxicity in general to the function or structure of the crucial organs.
31969608	10	9	theme	solution	2162:2169	arg1	pH					2171:2172	solution pH	2162:2172	solution pH in the slow release stage	2162:2198	The Korsmeyer-Peppas model is most suitable for the lovastatin release process from the alginate/chitosan/lovastatin nanoparticles in the first stage, and then the drug release complies with other models depending on solution pH in the slow release stage.
31969608	8	10	from	rate	1648:1651	arg1	nanoparticles					1678:1690	the nanoparticles	1674:1690	the nanoparticles	1674:1690	The rate of drug release from the nanoparticles is proportional to the increase in the solution pH and inversely proportional to the content of the loaded lovastatin.
31969608	3	11	theme	nanoparticles	556:568	arg1	spectrum					511:518	The FTIR spectrum	502:518	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles	502:568	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	13	12	theme	composite	2689:2697	arg1	nanoparticles					2699:2711	these composite nanoparticles	2683:2711	these composite nanoparticles	2683:2711	The results show that the ACL nanoparticles are safe in mice and that these composite nanoparticles might be useful as a new drug carrier.
31969608	7	13	theme	different	1490:1498	arg1	ratios					1518:1523	different alginate/chitosan ratios	1490:1523	different alginate/chitosan ratios	1490:1523	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	4	14	theme	laser	828:832	arg1	scattering					834:843	The laser scattering results and SEM	824:859	scattering	834:843	The laser scattering results and SEM images indicated that the alginate/chitosan/lovastatin nanoparticles have a spherical shape with a particle size in the range of 50-80 nm.
31969608	7	15	theme	alginate/chitosan/lovastatin	1409:1436	arg1	nanoparticles					1438:1450	the alginate/chitosan/lovastatin nanoparticles	1405:1450	the alginate/chitosan/lovastatin nanoparticles	1405:1450	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	3	16	theme	OH	757:758	arg1	groups					760:765	the C-O, NH, and OH groups	740:765	groups	760:765	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	12	17	with	test	2430:2433	arg1	treatments					2440:2449	treatments	2440:2449	treatments of 100 mg/kg and 300 mg/kg ACL nanoparticles	2440:2494	The LD50 of the drug was higher than 5000 mg/kg, while in the subchronic toxicity test with treatments of 100 mg/kg and 300 mg/kg ACL nanoparticles, there were no abnormal signs, mortality, or toxicity in general to the function or structure of the crucial organs.
31969608	1	18	theme	alginate/chitosan	188:204	arg1	nanoparticles					206:218	alginate/chitosan nanoparticles	188:218	alginate/chitosan nanoparticles to load the drug lovastatin	188:246	In this study, chitosan and alginate were selected to prepare alginate/chitosan nanoparticles to load the drug lovastatin by the ionic gelation method.
31969608	10	19	theme	slow	2181:2184	arg1	stage					2194:2198	the slow release stage	2177:2198	the slow release stage	2177:2198	The Korsmeyer-Peppas model is most suitable for the lovastatin release process from the alginate/chitosan/lovastatin nanoparticles in the first stage, and then the drug release complies with other models depending on solution pH in the slow release stage.
31969608	3	20	from	NH	749:750	arg1	lovastatin					728:737	lovastatin	728:737	lovastatin	728:737	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	3	20	from	NH	749:750	arg1	chitosan					770:777	chitosan	770:777	chitosan	770:777	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	3	20	from	NH	749:750	arg1	alginate					814:821	alginate	814:821	alginate	814:821	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	8	21	theme	lovastatin	1799:1808	arg1	content					1777:1783	the content	1773:1783	the content of the loaded lovastatin	1773:1808	The rate of drug release from the nanoparticles is proportional to the increase in the solution pH and inversely proportional to the content of the loaded lovastatin.
31969608	7	22	theme	lovastatin	1381:1390	arg1	release					1392:1398	the in vitro lovastatin release	1368:1398	the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions	1368:1477	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	11	23	theme	acute	2327:2331	arg1	test					2342:2345	the acute toxicity test	2323:2345	the acute toxicity test	2323:2345	In addition, the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles was sufficiently low in mice in the acute toxicity test.
31969608	5	24	theme	alginate/chitosan/lovastatin	1063:1090	arg1	nanoparticles					1092:1104	the alginate/chitosan/lovastatin nanoparticles	1059:1104	the alginate/chitosan/lovastatin nanoparticles	1059:1104	The DSC diagrams displayed that the melting temperature of the alginate/chitosan/lovastatin nanoparticles was higher than that of chitosan and lower than that of alginate.
31969608	3	25	theme	hydrogen	639:646	arg1	bonding					648:654	hydrogen bonding	639:654	hydrogen bonding	639:654	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	7	26	theme	in	1372:1373	arg1	release					1392:1398	the in vitro lovastatin release	1368:1398	the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions	1368:1477	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	11	27	from	test	2342:2345	arg1	low					2308:2310	low	2308:2310	low	2308:2310	In addition, the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles was sufficiently low in mice in the acute toxicity test.
31969608	11	27	from	test	2342:2345	arg1	toxicity					2218:2225	the toxicity	2214:2225	the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles	2214:2289	In addition, the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles was sufficiently low in mice in the acute toxicity test.
31969608	11	28	from	low	2308:2310	arg1	mice					2315:2318	mice	2315:2318	mice	2315:2318	In addition, the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles was sufficiently low in mice in the acute toxicity test.
31969608	11	28	from	low	2308:2310	arg1	addition					2204:2211	addition	2204:2211	addition	2204:2211	In addition, the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles was sufficiently low in mice in the acute toxicity test.
31969608	11	28	from	low	2308:2310	arg1	test					2342:2345	the acute toxicity test	2323:2345	the acute toxicity test	2323:2345	In addition, the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles was sufficiently low in mice in the acute toxicity test.
31969608	11	29	from	addition	2204:2211	arg1	low					2308:2310	low	2308:2310	low	2308:2310	In addition, the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles was sufficiently low in mice in the acute toxicity test.
31969608	11	29	from	addition	2204:2211	arg1	toxicity					2218:2225	the toxicity	2214:2225	the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles	2214:2289	In addition, the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles was sufficiently low in mice in the acute toxicity test.
31969608	8	30	from	nanoparticles	1678:1690	arg1	proportional					1695:1706	proportional	1695:1706	proportional	1695:1706	The rate of drug release from the nanoparticles is proportional to the increase in the solution pH and inversely proportional to the content of the loaded lovastatin.
31969608	8	30	from	nanoparticles	1678:1690	arg1	rate					1648:1651	The rate	1644:1651	The rate of drug release from the nanoparticles	1644:1690	The rate of drug release from the nanoparticles is proportional to the increase in the solution pH and inversely proportional to the content of the loaded lovastatin.
31969608	8	30	from	nanoparticles	1678:1690	arg1	release					1661:1667	drug release	1656:1667	drug release from the nanoparticles	1656:1690	The rate of drug release from the nanoparticles is proportional to the increase in the solution pH and inversely proportional to the content of the loaded lovastatin.
31969608	5	31	theme	melting	1036:1042	arg1	temperature					1044:1054	the melting temperature	1032:1054	the melting temperature of the alginate/chitosan/lovastatin nanoparticles	1032:1104	The DSC diagrams displayed that the melting temperature of the alginate/chitosan/lovastatin nanoparticles was higher than that of chitosan and lower than that of alginate.
31969608	5	31	theme	melting	1036:1042	arg1	higher					1110:1115	higher	1110:1115	higher	1110:1115	The DSC diagrams displayed that the melting temperature of the alginate/chitosan/lovastatin nanoparticles was higher than that of chitosan and lower than that of alginate.
31969608	11	32	theme	abbreviated	2260:2270	arg1	alginate/chitosan/lovastatin					2230:2257	alginate/chitosan/lovastatin	2230:2257	alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles	2230:2289	In addition, the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles was sufficiently low in mice in the acute toxicity test.
31969608	11	32	theme	abbreviated	2260:2270	arg1	ACL					2272:2274	abbreviated ACL	2260:2274	abbreviated ACL	2260:2274	In addition, the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles was sufficiently low in mice in the acute toxicity test.
31969608	3	33	theme	C-O	787:789	arg1	C=O					792:794	the C-O, C=O, and OH groups	783:809	C=O	792:794	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	0	34	theme	toxic	90:94	arg1	effects					96:102	their toxic effects	84:102	their toxic effects	84:102	Characterization of chitosan/alginate/lovastatin nanoparticles and investigation of their toxic effects in vitro and in vivo.
31969608	9	35	theme	drug	1815:1818	arg1	process					1828:1834	The drug release process	1811:1834	The drug release process	1811:1834	The drug release process is divided into two stages: a rapid stage over the first 10 hr, then the release becomes gradual and stable.
31969608	6	36	contain	have	1266:1269	arg2	degree					1288:1293	a larger crystal degree	1271:1293	a larger crystal degree	1271:1293	This result means that the alginate and chitosan interact together, so that the nanoparticles have a larger crystal degree when compared with alginate and chitosan individually.
31969608	6	36	contain	have	1266:1269	arg1	nanoparticles					1252:1264	the nanoparticles	1248:1264	the nanoparticles	1248:1264	This result means that the alginate and chitosan interact together, so that the nanoparticles have a larger crystal degree when compared with alginate and chitosan individually.
31969608	12	37	theme	organs	2605:2610	arg1	structure					2580:2588	structure	2580:2588	structure	2580:2588	The LD50 of the drug was higher than 5000 mg/kg, while in the subchronic toxicity test with treatments of 100 mg/kg and 300 mg/kg ACL nanoparticles, there were no abnormal signs, mortality, or toxicity in general to the function or structure of the crucial organs.
31969608	12	37	theme	organs	2605:2610	arg1	function					2568:2575	function	2568:2575	function	2568:2575	The LD50 of the drug was higher than 5000 mg/kg, while in the subchronic toxicity test with treatments of 100 mg/kg and 300 mg/kg ACL nanoparticles, there were no abnormal signs, mortality, or toxicity in general to the function or structure of the crucial organs.
31969608	8	38	from	increase	1715:1722	arg1	pH					1740:1741	the solution pH	1727:1741	the solution pH	1727:1741	The rate of drug release from the nanoparticles is proportional to the increase in the solution pH and inversely proportional to the content of the loaded lovastatin.
31969608	2	39	theme	differential	453:464	arg1	calorimetry					475:485	differential scanning calorimetry	453:485	differential scanning calorimetry (DSC)	453:491	The synthesized nanoparticles loaded with drug were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), laser scattering and differential scanning calorimetry (DSC) methods.
31969608	2	39	theme	differential	453:464	arg1	DSC					488:490	DSC	488:490	DSC	488:490	The synthesized nanoparticles loaded with drug were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), laser scattering and differential scanning calorimetry (DSC) methods.
31969608	2	40	dep	Fourier	347:353	arg1	transform					355:363	transform	355:363	transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), laser scattering and differential scanning calorimetry (DSC) methods	355:499	The synthesized nanoparticles loaded with drug were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), laser scattering and differential scanning calorimetry (DSC) methods.
31969608	2	41	theme	scanning	396:403	arg1	SEM					426:428	SEM	426:428	SEM	426:428	The synthesized nanoparticles loaded with drug were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), laser scattering and differential scanning calorimetry (DSC) methods.
31969608	2	41	theme	scanning	396:403	arg1	microscopy					414:423	scanning electron microscopy	396:423	scanning electron microscopy (SEM)	396:429	The synthesized nanoparticles loaded with drug were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), laser scattering and differential scanning calorimetry (DSC) methods.
31969608	4	42	contain	have	930:933	arg1	nanoparticles					916:928	the alginate/chitosan/lovastatin nanoparticles	883:928	the alginate/chitosan/lovastatin nanoparticles	883:928	The laser scattering results and SEM images indicated that the alginate/chitosan/lovastatin nanoparticles have a spherical shape with a particle size in the range of 50-80 nm.
31969608	4	42	contain	have	930:933	arg2	shape					947:951	a spherical shape	935:951	a spherical shape	935:951	The laser scattering results and SEM images indicated that the alginate/chitosan/lovastatin nanoparticles have a spherical shape with a particle size in the range of 50-80 nm.
31969608	12	43	theme	100 mg/kg	2454:2462	arg1	treatments					2440:2449	treatments	2440:2449	treatments of 100 mg/kg and 300 mg/kg ACL nanoparticles	2440:2494	The LD50 of the drug was higher than 5000 mg/kg, while in the subchronic toxicity test with treatments of 100 mg/kg and 300 mg/kg ACL nanoparticles, there were no abnormal signs, mortality, or toxicity in general to the function or structure of the crucial organs.
31969608	0	44	theme	chitosan/alginate/lovastatin	20:47	arg1	Characterization					0:15	Characterization	0:15	Characterization of chitosan/alginate/lovastatin	0:47	Characterization of chitosan/alginate/lovastatin nanoparticles and investigation of their toxic effects in vitro and in vivo.
31969608	3	45	theme	alginate/chitosan/lovastatin	527:554	arg1	nanoparticles					556:568	the alginate/chitosan/lovastatin nanoparticles	523:568	the alginate/chitosan/lovastatin nanoparticles	523:568	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	9	46	theme	rapid	1866:1870	arg1	stage					1872:1876	a rapid stage	1864:1876	two stages: a rapid stage over the first 10 hr	1852:1897	The drug release process is divided into two stages: a rapid stage over the first 10 hr, then the release becomes gradual and stable.
31969608	12	47	theme	300 mg/kg	2468:2476	arg1	nanoparticles					2482:2494	300 mg/kg ACL nanoparticles	2468:2494	300 mg/kg ACL nanoparticles	2468:2494	The LD50 of the drug was higher than 5000 mg/kg, while in the subchronic toxicity test with treatments of 100 mg/kg and 300 mg/kg ACL nanoparticles, there were no abnormal signs, mortality, or toxicity in general to the function or structure of the crucial organs.
31969608	3	48	from	groups	804:809	arg1	lovastatin					728:737	lovastatin	728:737	lovastatin	728:737	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	3	48	from	groups	804:809	arg1	chitosan					770:777	chitosan	770:777	chitosan	770:777	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	3	48	from	groups	804:809	arg1	alginate					814:821	alginate	814:821	alginate	814:821	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	5	49	theme	DSC	1004:1006	arg1	diagrams					1008:1015	The DSC diagrams	1000:1015	The DSC diagrams	1000:1015	The DSC diagrams displayed that the melting temperature of the alginate/chitosan/lovastatin nanoparticles was higher than that of chitosan and lower than that of alginate.
31969608	13	50	theme	ACL	2639:2641	arg1	safe					2661:2664	safe	2661:2664	safe	2661:2664	The results show that the ACL nanoparticles are safe in mice and that these composite nanoparticles might be useful as a new drug carrier.
31969608	13	50	theme	ACL	2639:2641	arg1	nanoparticles					2643:2655	the ACL nanoparticles	2635:2655	the ACL nanoparticles	2635:2655	The results show that the ACL nanoparticles are safe in mice and that these composite nanoparticles might be useful as a new drug carrier.
31969608	10	51	from	pH	2171:2172	arg1	stage					2194:2198	the slow release stage	2177:2198	the slow release stage	2177:2198	The Korsmeyer-Peppas model is most suitable for the lovastatin release process from the alginate/chitosan/lovastatin nanoparticles in the first stage, and then the drug release complies with other models depending on solution pH in the slow release stage.
31969608	4	52	dep	scattering	834:843	arg1	results					845:851	results	845:851	results	845:851	The laser scattering results and SEM images indicated that the alginate/chitosan/lovastatin nanoparticles have a spherical shape with a particle size in the range of 50-80 nm.
31969608	7	53	theme	different	1559:1567	arg1	contents					1580:1587	different lovastatin contents	1559:1587	different lovastatin contents	1559:1587	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	9	54	theme	first	1887:1891	arg1	10 hr					1893:1897	the first 10 hr	1883:1897	the first 10 hr	1883:1897	The drug release process is divided into two stages: a rapid stage over the first 10 hr, then the release becomes gradual and stable.
31969608	3	55	from	C=O	792:794	arg1	lovastatin					728:737	lovastatin	728:737	lovastatin	728:737	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	3	55	from	C=O	792:794	arg1	chitosan					770:777	chitosan	770:777	chitosan	770:777	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	3	55	from	C=O	792:794	arg1	alginate					814:821	alginate	814:821	alginate	814:821	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	3	56	theme	OH	715:716	arg1	groups					718:723	the C-O, C=O, and OH groups	697:723	groups	718:723	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	1	57	dep	load	223:226	arg1	lovastatin					237:246	the drug lovastatin	228:246	the drug lovastatin	228:246	In this study, chitosan and alginate were selected to prepare alginate/chitosan nanoparticles to load the drug lovastatin by the ionic gelation method.
31969608	13	58	theme	new	2734:2736	arg1	carrier					2743:2749	a new drug carrier	2732:2749	a new drug carrier	2732:2749	The results show that the ACL nanoparticles are safe in mice and that these composite nanoparticles might be useful as a new drug carrier.
31969608	1	59	theme	drug	232:235	arg1	lovastatin					237:246	the drug lovastatin	228:246	the drug lovastatin	228:246	In this study, chitosan and alginate were selected to prepare alginate/chitosan nanoparticles to load the drug lovastatin by the ionic gelation method.
31969608	8	60	theme	drug	1656:1659	arg1	release					1661:1667	drug release	1656:1667	drug release from the nanoparticles	1656:1690	The rate of drug release from the nanoparticles is proportional to the increase in the solution pH and inversely proportional to the content of the loaded lovastatin.
31969608	3	61	from	C=O	706:708	arg1	lovastatin					728:737	lovastatin	728:737	lovastatin	728:737	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	3	61	from	C=O	706:708	arg1	chitosan					770:777	chitosan	770:777	chitosan	770:777	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	3	61	from	C=O	706:708	arg1	alginate					814:821	alginate	814:821	alginate	814:821	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	12	62	dep	function	2568:2575	arg1	the					2564:2566	the	2564:2566	the	2564:2566	The LD50 of the drug was higher than 5000 mg/kg, while in the subchronic toxicity test with treatments of 100 mg/kg and 300 mg/kg ACL nanoparticles, there were no abnormal signs, mortality, or toxicity in general to the function or structure of the crucial organs.
31969608	4	63	from	size	969:972	arg1	range					981:985	the range	977:985	the range of 50-80 nm	977:997	The laser scattering results and SEM images indicated that the alginate/chitosan/lovastatin nanoparticles have a spherical shape with a particle size in the range of 50-80 nm.
31969608	7	64	theme	solution	1536:1543	arg1	values					1548:1553	different solution pH values	1526:1553	different solution pH values	1526:1553	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	12	65	theme	subchronic	2410:2419	arg1	test					2430:2433	the subchronic toxicity test	2406:2433	the subchronic toxicity test with treatments of 100 mg/kg and 300 mg/kg ACL nanoparticles	2406:2494	The LD50 of the drug was higher than 5000 mg/kg, while in the subchronic toxicity test with treatments of 100 mg/kg and 300 mg/kg ACL nanoparticles, there were no abnormal signs, mortality, or toxicity in general to the function or structure of the crucial organs.
31969608	11	66	theme	alginate/chitosan/lovastatin	2230:2257	arg1	nanoparticles					2277:2289	alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles	2230:2289	alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles	2230:2289	In addition, the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles was sufficiently low in mice in the acute toxicity test.
31969608	6	67	theme	crystal	1280:1286	arg1	degree					1288:1293	a larger crystal degree	1271:1293	a larger crystal degree	1271:1293	This result means that the alginate and chitosan interact together, so that the nanoparticles have a larger crystal degree when compared with alginate and chitosan individually.
31969608	10	68	theme	Korsmeyer-Peppas	1949:1964	arg1	model					1966:1970	The Korsmeyer-Peppas model	1945:1970	The Korsmeyer-Peppas model	1945:1970	The Korsmeyer-Peppas model is most suitable for the lovastatin release process from the alginate/chitosan/lovastatin nanoparticles in the first stage, and then the drug release complies with other models depending on solution pH in the slow release stage.
31969608	10	68	theme	Korsmeyer-Peppas	1949:1964	arg1	suitable					1980:1987	suitable	1980:1987	suitable	1980:1987	The Korsmeyer-Peppas model is most suitable for the lovastatin release process from the alginate/chitosan/lovastatin nanoparticles in the first stage, and then the drug release complies with other models depending on solution pH in the slow release stage.
31969608	3	69	theme	FTIR	506:509	arg1	spectrum					511:518	The FTIR spectrum	502:518	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles	502:568	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	8	70	theme	solution	1731:1738	arg1	pH					1740:1741	the solution pH	1727:1741	the solution pH	1727:1741	The rate of drug release from the nanoparticles is proportional to the increase in the solution pH and inversely proportional to the content of the loaded lovastatin.
31969608	1	71	theme	ionic	255:259	arg1	method					270:275	the ionic gelation method	251:275	the ionic gelation method	251:275	In this study, chitosan and alginate were selected to prepare alginate/chitosan nanoparticles to load the drug lovastatin by the ionic gelation method.
31969608	4	72	theme	scattering	834:843	arg1	images					861:866	The laser scattering results and SEM images	824:866	The laser scattering results and SEM images	824:866	The laser scattering results and SEM images indicated that the alginate/chitosan/lovastatin nanoparticles have a spherical shape with a particle size in the range of 50-80 nm.
31969608	7	73	theme	alginate/chitosan	1500:1516	arg1	ratios					1518:1523	different alginate/chitosan ratios	1490:1523	different alginate/chitosan ratios	1490:1523	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	12	74	theme	abnormal	2511:2518	arg1	signs					2520:2524	no abnormal signs	2508:2524	no abnormal signs	2508:2524	The LD50 of the drug was higher than 5000 mg/kg, while in the subchronic toxicity test with treatments of 100 mg/kg and 300 mg/kg ACL nanoparticles, there were no abnormal signs, mortality, or toxicity in general to the function or structure of the crucial organs.
31969608	2	75	theme	synthesized	282:292	arg1	nanoparticles					294:306	The synthesized nanoparticles	278:306	The synthesized nanoparticles loaded with drug	278:323	The synthesized nanoparticles loaded with drug were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), laser scattering and differential scanning calorimetry (DSC) methods.
31969608	7	76	dep	in	1372:1373	arg1	vitro					1375:1379	vitro	1375:1379	vitro	1375:1379	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	2	77	theme	scanning	466:473	arg1	calorimetry					475:485	differential scanning calorimetry	453:485	differential scanning calorimetry (DSC)	453:491	The synthesized nanoparticles loaded with drug were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), laser scattering and differential scanning calorimetry (DSC) methods.
31969608	2	77	theme	scanning	466:473	arg1	DSC					488:490	DSC	488:490	DSC	488:490	The synthesized nanoparticles loaded with drug were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), laser scattering and differential scanning calorimetry (DSC) methods.
31969608	7	78	theme	different	1458:1466	arg1	conditions					1468:1477	different conditions	1458:1477	different conditions	1458:1477	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	3	79	theme	C-O	744:746	arg1	NH					749:750	the C-O, NH, and OH groups	740:765	NH	749:750	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	10	80	theme	lovastatin	1997:2006	arg1	process					2016:2022	the lovastatin release process	1993:2022	the lovastatin release process from the alginate/chitosan/lovastatin nanoparticles in the first stage	1993:2093	The Korsmeyer-Peppas model is most suitable for the lovastatin release process from the alginate/chitosan/lovastatin nanoparticles in the first stage, and then the drug release complies with other models depending on solution pH in the slow release stage.
31969608	10	81	theme	release	2186:2192	arg1	stage					2194:2198	the slow release stage	2177:2198	the slow release stage	2177:2198	The Korsmeyer-Peppas model is most suitable for the lovastatin release process from the alginate/chitosan/lovastatin nanoparticles in the first stage, and then the drug release complies with other models depending on solution pH in the slow release stage.
31969608	4	82	theme	alginate/chitosan/lovastatin	887:914	arg1	nanoparticles					916:928	the alginate/chitosan/lovastatin nanoparticles	883:928	the alginate/chitosan/lovastatin nanoparticles	883:928	The laser scattering results and SEM images indicated that the alginate/chitosan/lovastatin nanoparticles have a spherical shape with a particle size in the range of 50-80 nm.
31969608	12	83	theme	drug	2364:2367	arg1	LD50					2352:2355	The LD50	2348:2355	The LD50 of the drug	2348:2367	The LD50 of the drug was higher than 5000 mg/kg, while in the subchronic toxicity test with treatments of 100 mg/kg and 300 mg/kg ACL nanoparticles, there were no abnormal signs, mortality, or toxicity in general to the function or structure of the crucial organs.
31969608	12	83	theme	drug	2364:2367	arg1	higher					2373:2378	higher	2373:2378	higher	2373:2378	The LD50 of the drug was higher than 5000 mg/kg, while in the subchronic toxicity test with treatments of 100 mg/kg and 300 mg/kg ACL nanoparticles, there were no abnormal signs, mortality, or toxicity in general to the function or structure of the crucial organs.
31969608	11	84	theme	toxicity	2333:2340	arg1	test					2342:2345	the acute toxicity test	2323:2345	the acute toxicity test	2323:2345	In addition, the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles was sufficiently low in mice in the acute toxicity test.
31969608	3	85	from	groups	718:723	arg1	lovastatin					728:737	lovastatin	728:737	lovastatin	728:737	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	3	85	from	groups	718:723	arg1	chitosan					770:777	chitosan	770:777	chitosan	770:777	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	3	85	from	groups	718:723	arg1	alginate					814:821	alginate	814:821	alginate	814:821	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	8	86	theme	loaded	1792:1797	arg1	lovastatin					1799:1808	the loaded lovastatin	1788:1808	the loaded lovastatin	1788:1808	The rate of drug release from the nanoparticles is proportional to the increase in the solution pH and inversely proportional to the content of the loaded lovastatin.
31969608	7	87	theme	release	1392:1398	arg1	values					1548:1553	different solution pH values	1526:1553	different solution pH values	1526:1553	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	7	87	theme	release	1392:1398	arg1	ratios					1518:1523	different alginate/chitosan ratios	1490:1523	different alginate/chitosan ratios	1490:1523	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	7	87	theme	release	1392:1398	arg1	Investigations					1350:1363	Investigations	1350:1363	Investigations	1350:1363	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	7	87	theme	release	1392:1398	arg1	contents					1580:1587	different lovastatin contents	1559:1587	different lovastatin contents	1559:1587	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	5	88	theme	nanoparticles	1092:1104	arg1	temperature					1044:1054	the melting temperature	1032:1054	the melting temperature of the alginate/chitosan/lovastatin nanoparticles	1032:1104	The DSC diagrams displayed that the melting temperature of the alginate/chitosan/lovastatin nanoparticles was higher than that of chitosan and lower than that of alginate.
31969608	5	88	theme	nanoparticles	1092:1104	arg1	higher					1110:1115	higher	1110:1115	higher	1110:1115	The DSC diagrams displayed that the melting temperature of the alginate/chitosan/lovastatin nanoparticles was higher than that of chitosan and lower than that of alginate.
31969608	10	89	theme	release	2008:2014	arg1	process					2016:2022	the lovastatin release process	1993:2022	the lovastatin release process from the alginate/chitosan/lovastatin nanoparticles in the first stage	1993:2093	The Korsmeyer-Peppas model is most suitable for the lovastatin release process from the alginate/chitosan/lovastatin nanoparticles in the first stage, and then the drug release complies with other models depending on solution pH in the slow release stage.
31969608	3	90	theme	dipolar-dipolar	660:674	arg1	interactions					676:687	dipolar-dipolar interactions	660:687	dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate	660:821	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	6	91	dep	alginate	1199:1206	arg1	the					1195:1197	the	1195:1197	the	1195:1197	This result means that the alginate and chitosan interact together, so that the nanoparticles have a larger crystal degree when compared with alginate and chitosan individually.
31969608	0	92	theme	effects	96:102	arg1	investigation					67:79	and investigation	63:79	investigation	67:79	Characterization of chitosan/alginate/lovastatin nanoparticles and investigation of their toxic effects in vitro and in vivo.
31969608	11	93	from	mice	2315:2318	arg1	low					2308:2310	low	2308:2310	low	2308:2310	In addition, the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles was sufficiently low in mice in the acute toxicity test.
31969608	11	93	from	mice	2315:2318	arg1	toxicity					2218:2225	the toxicity	2214:2225	the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles	2214:2289	In addition, the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles was sufficiently low in mice in the acute toxicity test.
31969608	7	94	from	nanoparticles	1438:1450	arg1	values					1548:1553	different solution pH values	1526:1553	different solution pH values	1526:1553	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	7	94	from	nanoparticles	1438:1450	arg1	ratios					1518:1523	different alginate/chitosan ratios	1490:1523	different alginate/chitosan ratios	1490:1523	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	7	94	from	nanoparticles	1438:1450	arg1	Investigations					1350:1363	Investigations	1350:1363	Investigations	1350:1363	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	7	94	from	nanoparticles	1438:1450	arg1	contents					1580:1587	different lovastatin contents	1559:1587	different lovastatin contents	1559:1587	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	7	94	from	nanoparticles	1438:1450	arg1	release					1392:1398	the in vitro lovastatin release	1368:1398	the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions	1368:1477	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	4	95	theme	particle	960:967	arg1	size					969:972	a particle size	958:972	a particle size in the range of 50-80 nm	958:997	The laser scattering results and SEM images indicated that the alginate/chitosan/lovastatin nanoparticles have a spherical shape with a particle size in the range of 50-80 nm.
31969608	10	96	theme	alginate/chitosan/lovastatin	2033:2060	arg1	nanoparticles					2062:2074	the alginate/chitosan/lovastatin nanoparticles	2029:2074	the alginate/chitosan/lovastatin nanoparticles in the first stage	2029:2093	The Korsmeyer-Peppas model is most suitable for the lovastatin release process from the alginate/chitosan/lovastatin nanoparticles in the first stage, and then the drug release complies with other models depending on solution pH in the slow release stage.
31969608	7	97	theme	ultraviolet-visible	1610:1628	arg1	spectroscopy					1630:1641	ultraviolet-visible spectroscopy	1610:1641	ultraviolet-visible spectroscopy	1610:1641	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	9	98	theme	release	1820:1826	arg1	process					1828:1834	The drug release process	1811:1834	The drug release process	1811:1834	The drug release process is divided into two stages: a rapid stage over the first 10 hr, then the release becomes gradual and stable.
31969608	12	99	theme	crucial	2597:2603	arg1	organs					2605:2610	the crucial organs	2593:2610	the crucial organs	2593:2610	The LD50 of the drug was higher than 5000 mg/kg, while in the subchronic toxicity test with treatments of 100 mg/kg and 300 mg/kg ACL nanoparticles, there were no abnormal signs, mortality, or toxicity in general to the function or structure of the crucial organs.
31969608	4	100	theme	spherical	937:945	arg1	shape					947:951	a spherical shape	935:951	a spherical shape	935:951	The laser scattering results and SEM images indicated that the alginate/chitosan/lovastatin nanoparticles have a spherical shape with a particle size in the range of 50-80 nm.
31969608	2	101	dep	spectroscopy	374:385	arg1	methods					493:499	methods	493:499	methods	493:499	The synthesized nanoparticles loaded with drug were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), laser scattering and differential scanning calorimetry (DSC) methods.
31969608	3	102	theme	OH	801:802	arg1	groups					804:809	the C-O, C=O, and OH groups	783:809	groups	804:809	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	7	103	from	Investigations	1350:1363	arg1	nanoparticles					1438:1450	the alginate/chitosan/lovastatin nanoparticles	1405:1450	the alginate/chitosan/lovastatin nanoparticles	1405:1450	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	10	104	theme	first	2083:2087	arg1	stage					2089:2093	the first stage	2079:2093	the first stage	2079:2093	The Korsmeyer-Peppas model is most suitable for the lovastatin release process from the alginate/chitosan/lovastatin nanoparticles in the first stage, and then the drug release complies with other models depending on solution pH in the slow release stage.
31969608	2	105	theme	electron	405:412	arg1	SEM					426:428	SEM	426:428	SEM	426:428	The synthesized nanoparticles loaded with drug were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), laser scattering and differential scanning calorimetry (DSC) methods.
31969608	2	105	theme	electron	405:412	arg1	microscopy					414:423	scanning electron microscopy	396:423	scanning electron microscopy (SEM)	396:429	The synthesized nanoparticles loaded with drug were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), laser scattering and differential scanning calorimetry (DSC) methods.
31969608	3	106	from	groups	760:765	arg1	lovastatin					728:737	lovastatin	728:737	lovastatin	728:737	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	3	106	from	groups	760:765	arg1	chitosan					770:777	chitosan	770:777	chitosan	770:777	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	3	106	from	groups	760:765	arg1	alginate					814:821	alginate	814:821	alginate	814:821	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	10	107	from	nanoparticles	2062:2074	arg1	process					2016:2022	the lovastatin release process	1993:2022	the lovastatin release process from the alginate/chitosan/lovastatin nanoparticles in the first stage	1993:2093	The Korsmeyer-Peppas model is most suitable for the lovastatin release process from the alginate/chitosan/lovastatin nanoparticles in the first stage, and then the drug release complies with other models depending on solution pH in the slow release stage.
31969608	10	107	from	nanoparticles	2062:2074	arg1	stage					2089:2093	the first stage	2079:2093	the first stage	2079:2093	The Korsmeyer-Peppas model is most suitable for the lovastatin release process from the alginate/chitosan/lovastatin nanoparticles in the first stage, and then the drug release complies with other models depending on solution pH in the slow release stage.
31969608	13	108	from	mice	2669:2672	arg1	safe					2661:2664	safe	2661:2664	safe	2661:2664	The results show that the ACL nanoparticles are safe in mice and that these composite nanoparticles might be useful as a new drug carrier.
31969608	13	108	from	mice	2669:2672	arg1	nanoparticles					2643:2655	the ACL nanoparticles	2635:2655	the ACL nanoparticles	2635:2655	The results show that the ACL nanoparticles are safe in mice and that these composite nanoparticles might be useful as a new drug carrier.
31969608	2	109	theme	laser	432:436	arg1	scattering					438:447	laser scattering	432:447	laser scattering	432:447	The synthesized nanoparticles loaded with drug were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), laser scattering and differential scanning calorimetry (DSC) methods.
31969608	12	110	theme	ACL	2478:2480	arg1	nanoparticles					2482:2494	300 mg/kg ACL nanoparticles	2468:2494	300 mg/kg ACL nanoparticles	2468:2494	The LD50 of the drug was higher than 5000 mg/kg, while in the subchronic toxicity test with treatments of 100 mg/kg and 300 mg/kg ACL nanoparticles, there were no abnormal signs, mortality, or toxicity in general to the function or structure of the crucial organs.
31969608	3	111	theme	C-O	701:703	arg1	C=O					706:708	the C-O, C=O, and OH groups	697:723	C=O	706:708	The FTIR spectrum of the alginate/chitosan/lovastatin nanoparticles showed that chitosan and alginate interacted with lovastatin through hydrogen bonding and dipolar-dipolar interactions between the C-O, C=O, and OH groups in lovastatin, the C-O, NH, and OH groups in chitosan and the C-O, C=O, and OH groups in alginate.
31969608	2	112	dep	transform	355:363	arg1	infrared					365:372	infrared	365:372	transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), laser scattering and differential scanning calorimetry (DSC) methods	355:499	The synthesized nanoparticles loaded with drug were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), laser scattering and differential scanning calorimetry (DSC) methods.
31969608	11	113	theme	nanoparticles	2277:2289	arg1	low					2308:2310	low	2308:2310	low	2308:2310	In addition, the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles was sufficiently low in mice in the acute toxicity test.
31969608	11	113	theme	nanoparticles	2277:2289	arg1	toxicity					2218:2225	the toxicity	2214:2225	the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles	2214:2289	In addition, the toxicity of alginate/chitosan/lovastatin (abbreviated ACL) nanoparticles was sufficiently low in mice in the acute toxicity test.
31969608	4	114	theme	50-80 nm	990:997	arg1	range					981:985	the range	977:985	the range of 50-80 nm	977:997	The laser scattering results and SEM images indicated that the alginate/chitosan/lovastatin nanoparticles have a spherical shape with a particle size in the range of 50-80 nm.
31969608	7	115	theme	lovastatin	1569:1578	arg1	contents					1580:1587	different lovastatin contents	1559:1587	different lovastatin contents	1559:1587	Investigations of the in vitro lovastatin release from the alginate/chitosan/lovastatin nanoparticles under different conditions, including different alginate/chitosan ratios, different solution pH values and different lovastatin contents, were carried out by ultraviolet-visible spectroscopy.
31969608	10	116	theme	drug	2109:2112	arg1	release					2114:2120	then the drug release	2100:2120	then the drug release	2100:2120	The Korsmeyer-Peppas model is most suitable for the lovastatin release process from the alginate/chitosan/lovastatin nanoparticles in the first stage, and then the drug release complies with other models depending on solution pH in the slow release stage.
31969608	8	117	theme	release	1661:1667	arg1	proportional					1695:1706	proportional	1695:1706	proportional	1695:1706	The rate of drug release from the nanoparticles is proportional to the increase in the solution pH and inversely proportional to the content of the loaded lovastatin.
31969608	8	117	theme	release	1661:1667	arg1	rate					1648:1651	The rate	1644:1651	The rate of drug release from the nanoparticles	1644:1690	The rate of drug release from the nanoparticles is proportional to the increase in the solution pH and inversely proportional to the content of the loaded lovastatin.
31969608	13	118	theme	drug	2738:2741	arg1	carrier					2743:2749	a new drug carrier	2732:2749	a new drug carrier	2732:2749	The results show that the ACL nanoparticles are safe in mice and that these composite nanoparticles might be useful as a new drug carrier.
31969608	12	119	theme	toxicity	2421:2428	arg1	test					2430:2433	the subchronic toxicity test	2406:2433	the subchronic toxicity test with treatments of 100 mg/kg and 300 mg/kg ACL nanoparticles	2406:2494	The LD50 of the drug was higher than 5000 mg/kg, while in the subchronic toxicity test with treatments of 100 mg/kg and 300 mg/kg ACL nanoparticles, there were no abnormal signs, mortality, or toxicity in general to the function or structure of the crucial organs.
33287322	5	0	theme	dispersive	934:943	arg1	EDX					965:967	EDX	965:967	EDX	965:967	The elemental composition of the hydrogels investigated using energy dispersive X-ray spectrometry (EDX) revealed the presence of minerals, such as magnesium, sodium, aluminum, and silicon in the SAHA aerogels.
33287322	5	0	theme	dispersive	934:943	arg1	spectrometry					951:962	energy dispersive X-ray spectrometry	927:962	energy dispersive X-ray spectrometry (EDX)	927:968	The elemental composition of the hydrogels investigated using energy dispersive X-ray spectrometry (EDX) revealed the presence of minerals, such as magnesium, sodium, aluminum, and silicon in the SAHA aerogels.
33287322	0	1	theme	Separation	81:90	arg1	Applications					92:103	Oil/Water Separation Applications	71:103	Oil/Water Separation Applications	71:103	Alginate-Halloysite Nanocomposite Aerogel: Preparation, Structure, and Oil/Water Separation Applications.
33287322	5	2	theme	SAHA	1061:1064	arg1	aerogels					1066:1073	the SAHA aerogels	1057:1073	the SAHA aerogels	1057:1073	The elemental composition of the hydrogels investigated using energy dispersive X-ray spectrometry (EDX) revealed the presence of minerals, such as magnesium, sodium, aluminum, and silicon in the SAHA aerogels.
33287322	3	3	theme	scanning	547:554	arg1	SEM					577:579	SEM	577:579	SEM	577:579	The as-prepared SAHA aerogels were characterized in detail by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transformation infrared (FT-IR) spectroscopy.
33287322	3	3	theme	scanning	547:554	arg1	microscopy					565:574	scanning electron microscopy	547:574	scanning electron microscopy (SEM)	547:580	The as-prepared SAHA aerogels were characterized in detail by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transformation infrared (FT-IR) spectroscopy.
33287322	2	4	theme	environmentally	272:286	arg1	aerogel					361:367	low-cost, and stable sodium alginate-halloysite clay composite aerogel	298:367	low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA)	298:374	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	2	4	theme	environmentally	272:286	arg1	friendly					288:295	an environmentally friendly	269:295	an environmentally friendly	269:295	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	7	5	theme	excellent	1321:1329	arg1	separation					1341:1350	excellent oil/water separation	1321:1350	excellent oil/water separation	1321:1350	The ease of fabrication, excellent oil/water separation, and multiple performances make the SAHA aerogel an interesting candidate for practical applications in water recycling.
33287322	5	6	theme	energy	927:932	arg1	EDX					965:967	EDX	965:967	EDX	965:967	The elemental composition of the hydrogels investigated using energy dispersive X-ray spectrometry (EDX) revealed the presence of minerals, such as magnesium, sodium, aluminum, and silicon in the SAHA aerogels.
33287322	5	6	theme	energy	927:932	arg1	spectrometry					951:962	energy dispersive X-ray spectrometry	927:962	energy dispersive X-ray spectrometry (EDX)	927:968	The elemental composition of the hydrogels investigated using energy dispersive X-ray spectrometry (EDX) revealed the presence of minerals, such as magnesium, sodium, aluminum, and silicon in the SAHA aerogels.
33287322	3	7	theme	force	590:594	arg1	AFM					608:610	AFM	608:610	AFM	608:610	The as-prepared SAHA aerogels were characterized in detail by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transformation infrared (FT-IR) spectroscopy.
33287322	3	7	theme	force	590:594	arg1	microscopy					596:605	atomic force microscopy	583:605	atomic force microscopy (AFM)	583:611	The as-prepared SAHA aerogels were characterized in detail by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transformation infrared (FT-IR) spectroscopy.
33287322	4	8	theme	three-dimensional	746:762	arg1	microstructure					771:784	a three-dimensional porous microstructure	744:784	a three-dimensional porous microstructure with uniformly dispersed halloysite nanotubes (HA) within the alginate matrix	744:862	Characterization of the SAHA aerogels revealed a three-dimensional porous microstructure with uniformly dispersed halloysite nanotubes (HA) within the alginate matrix.
33287322	6	9	theme	excellent	1239:1247	arg1	%					1292:1292	up to 99.7%	1282:1292	up to 99.7%	1282:1292	The presence of a hydrophilic alginate matrix combined with these unique morphological characteristics resulted in SAHA aerogels with underwater oleophobicity and excellent oil/water separation efficiency (up to 99.7%).
33287322	6	9	theme	excellent	1239:1247	arg1	efficiency					1270:1279	excellent oil/water separation efficiency	1239:1279	excellent oil/water separation efficiency (up to 99.7%)	1239:1293	The presence of a hydrophilic alginate matrix combined with these unique morphological characteristics resulted in SAHA aerogels with underwater oleophobicity and excellent oil/water separation efficiency (up to 99.7%).
33287322	6	10	theme	hydrophilic	1094:1104	arg1	matrix					1115:1120	a hydrophilic alginate matrix	1092:1120	a hydrophilic alginate matrix combined with these unique morphological characteristics	1092:1177	The presence of a hydrophilic alginate matrix combined with these unique morphological characteristics resulted in SAHA aerogels with underwater oleophobicity and excellent oil/water separation efficiency (up to 99.7%).
33287322	4	11	theme	aerogels	726:733	arg1	Characterization					697:712	Characterization	697:712	Characterization of the SAHA aerogels	697:733	Characterization of the SAHA aerogels revealed a three-dimensional porous microstructure with uniformly dispersed halloysite nanotubes (HA) within the alginate matrix.
33287322	4	12	theme	SAHA	721:724	arg1	aerogels					726:733	the SAHA aerogels	717:733	the SAHA aerogels	717:733	Characterization of the SAHA aerogels revealed a three-dimensional porous microstructure with uniformly dispersed halloysite nanotubes (HA) within the alginate matrix.
33287322	7	13	from	applications	1440:1451	arg1	recycling					1462:1470	water recycling	1456:1470	water recycling	1456:1470	The ease of fabrication, excellent oil/water separation, and multiple performances make the SAHA aerogel an interesting candidate for practical applications in water recycling.
33287322	7	14	theme	oil/water	1331:1339	arg1	separation					1341:1350	excellent oil/water separation	1321:1350	excellent oil/water separation	1321:1350	The ease of fabrication, excellent oil/water separation, and multiple performances make the SAHA aerogel an interesting candidate for practical applications in water recycling.
33287322	2	15	theme	low-cost	298:305	arg1	SAHA					370:373	SAHA	370:373	SAHA	370:373	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	2	15	theme	low-cost	298:305	arg1	aerogel					361:367	low-cost, and stable sodium alginate-halloysite clay composite aerogel	298:367	low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA)	298:374	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	2	15	theme	low-cost	298:305	arg1	friendly					288:295	an environmentally friendly	269:295	an environmentally friendly	269:295	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	7	16	theme	separation	1341:1350	arg1	ease					1300:1303	The ease	1296:1303	The ease of fabrication, excellent oil/water separation, and multiple performances	1296:1377	The ease of fabrication, excellent oil/water separation, and multiple performances make the SAHA aerogel an interesting candidate for practical applications in water recycling.
33287322	2	17	theme	synthesis	416:424	arg1	procedure					426:434	a two-step synthesis procedure	405:434	a two-step synthesis procedure	405:434	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	5	18	theme	X-ray	945:949	arg1	EDX					965:967	EDX	965:967	EDX	965:967	The elemental composition of the hydrogels investigated using energy dispersive X-ray spectrometry (EDX) revealed the presence of minerals, such as magnesium, sodium, aluminum, and silicon in the SAHA aerogels.
33287322	5	18	theme	X-ray	945:949	arg1	spectrometry					951:962	energy dispersive X-ray spectrometry	927:962	energy dispersive X-ray spectrometry (EDX)	927:968	The elemental composition of the hydrogels investigated using energy dispersive X-ray spectrometry (EDX) revealed the presence of minerals, such as magnesium, sodium, aluminum, and silicon in the SAHA aerogels.
33287322	4	19	theme	dispersed	801:809	arg1	HA					833:834	HA	833:834	HA	833:834	Characterization of the SAHA aerogels revealed a three-dimensional porous microstructure with uniformly dispersed halloysite nanotubes (HA) within the alginate matrix.
33287322	4	19	theme	dispersed	801:809	arg1	nanotubes					822:830	uniformly dispersed halloysite nanotubes	791:830	uniformly dispersed halloysite nanotubes (HA) within the alginate matrix	791:862	Characterization of the SAHA aerogels revealed a three-dimensional porous microstructure with uniformly dispersed halloysite nanotubes (HA) within the alginate matrix.
33287322	1	20	theme	water	215:219	arg1	pollution					221:229	water pollution	215:229	especially water pollution	204:229	Environmental remediation using green approaches for addressing various pollution-related issues, especially water pollution, is in high demand.
33287322	1	20	theme	water	215:219	arg1	approaches					144:153	green approaches	138:153	green approaches for addressing various pollution-related issues	138:201	Environmental remediation using green approaches for addressing various pollution-related issues, especially water pollution, is in high demand.
33287322	7	21	theme	fabrication	1308:1318	arg1	ease					1300:1303	The ease	1296:1303	The ease of fabrication, excellent oil/water separation, and multiple performances	1296:1377	The ease of fabrication, excellent oil/water separation, and multiple performances make the SAHA aerogel an interesting candidate for practical applications in water recycling.
33287322	6	22	dep	99.7	1288:1291	arg1	to					1285:1286	to	1285:1286	to	1285:1286	The presence of a hydrophilic alginate matrix combined with these unique morphological characteristics resulted in SAHA aerogels with underwater oleophobicity and excellent oil/water separation efficiency (up to 99.7%).
33287322	2	23	theme	clay	346:349	arg1	SAHA					370:373	SAHA	370:373	SAHA	370:373	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	2	23	theme	clay	346:349	arg1	aerogel					361:367	low-cost, and stable sodium alginate-halloysite clay composite aerogel	298:367	low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA)	298:374	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	2	23	theme	clay	346:349	arg1	friendly					288:295	an environmentally friendly	269:295	an environmentally friendly	269:295	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	5	24	theme	hydrogels	898:906	arg1	composition					879:889	The elemental composition	865:889	The elemental composition of the hydrogels investigated using energy dispersive X-ray spectrometry (EDX)	865:968	The elemental composition of the hydrogels investigated using energy dispersive X-ray spectrometry (EDX) revealed the presence of minerals, such as magnesium, sodium, aluminum, and silicon in the SAHA aerogels.
33287322	6	25	with	aerogels	1196:1203	arg1	%					1292:1292	up to 99.7%	1282:1292	up to 99.7%	1282:1292	The presence of a hydrophilic alginate matrix combined with these unique morphological characteristics resulted in SAHA aerogels with underwater oleophobicity and excellent oil/water separation efficiency (up to 99.7%).
33287322	6	25	with	aerogels	1196:1203	arg1	efficiency					1270:1279	excellent oil/water separation efficiency	1239:1279	excellent oil/water separation efficiency (up to 99.7%)	1239:1293	The presence of a hydrophilic alginate matrix combined with these unique morphological characteristics resulted in SAHA aerogels with underwater oleophobicity and excellent oil/water separation efficiency (up to 99.7%).
33287322	6	25	with	aerogels	1196:1203	arg1	oleophobicity					1221:1233	underwater oleophobicity	1210:1233	underwater oleophobicity	1210:1233	The presence of a hydrophilic alginate matrix combined with these unique morphological characteristics resulted in SAHA aerogels with underwater oleophobicity and excellent oil/water separation efficiency (up to 99.7%).
33287322	1	26	theme	Environmental	106:118	arg1	remediation					120:130	Environmental remediation	106:130	Environmental remediation using green approaches for addressing various pollution-related issues, especially water pollution,	106:230	Environmental remediation using green approaches for addressing various pollution-related issues, especially water pollution, is in high demand.
33287322	7	27	theme	SAHA	1388:1391	arg1	aerogel					1393:1399	the SAHA aerogel	1384:1399	the SAHA aerogel	1384:1399	The ease of fabrication, excellent oil/water separation, and multiple performances make the SAHA aerogel an interesting candidate for practical applications in water recycling.
33287322	5	28	theme	elemental	869:877	arg1	composition					879:889	The elemental composition	865:889	The elemental composition of the hydrogels investigated using energy dispersive X-ray spectrometry (EDX)	865:968	The elemental composition of the hydrogels investigated using energy dispersive X-ray spectrometry (EDX) revealed the presence of minerals, such as magnesium, sodium, aluminum, and silicon in the SAHA aerogels.
33287322	4	29	theme	alginate	848:855	arg1	matrix					857:862	the alginate matrix	844:862	the alginate matrix	844:862	Characterization of the SAHA aerogels revealed a three-dimensional porous microstructure with uniformly dispersed halloysite nanotubes (HA) within the alginate matrix.
33287322	2	30	theme	sodium	319:324	arg1	SAHA					370:373	SAHA	370:373	SAHA	370:373	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	2	30	theme	sodium	319:324	arg1	aerogel					361:367	low-cost, and stable sodium alginate-halloysite clay composite aerogel	298:367	low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA)	298:374	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	2	30	theme	sodium	319:324	arg1	friendly					288:295	an environmentally friendly	269:295	an environmentally friendly	269:295	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	2	31	theme	ionic	447:451	arg1	crosslinking					453:464	ionic crosslinking	447:464	ionic crosslinking	447:464	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	2	32	theme	alginate-halloysite	326:344	arg1	SAHA					370:373	SAHA	370:373	SAHA	370:373	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	2	32	theme	alginate-halloysite	326:344	arg1	aerogel					361:367	low-cost, and stable sodium alginate-halloysite clay composite aerogel	298:367	low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA)	298:374	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	2	32	theme	alginate-halloysite	326:344	arg1	friendly					288:295	an environmentally friendly	269:295	an environmentally friendly	269:295	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	6	33	theme	underwater	1210:1219	arg1	oleophobicity					1221:1233	underwater oleophobicity	1210:1233	underwater oleophobicity	1210:1233	The presence of a hydrophilic alginate matrix combined with these unique morphological characteristics resulted in SAHA aerogels with underwater oleophobicity and excellent oil/water separation efficiency (up to 99.7%).
33287322	4	34	theme	halloysite	811:820	arg1	HA					833:834	HA	833:834	HA	833:834	Characterization of the SAHA aerogels revealed a three-dimensional porous microstructure with uniformly dispersed halloysite nanotubes (HA) within the alginate matrix.
33287322	4	34	theme	halloysite	811:820	arg1	nanotubes					822:830	uniformly dispersed halloysite nanotubes	791:830	uniformly dispersed halloysite nanotubes (HA) within the alginate matrix	791:862	Characterization of the SAHA aerogels revealed a three-dimensional porous microstructure with uniformly dispersed halloysite nanotubes (HA) within the alginate matrix.
33287322	3	35	theme	Fourier	643:649	arg1	spectroscopy					683:694	Fourier transformation infrared (FT-IR) spectroscopy	643:694	Fourier transformation infrared (FT-IR) spectroscopy	643:694	The as-prepared SAHA aerogels were characterized in detail by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transformation infrared (FT-IR) spectroscopy.
33287322	2	36	theme	stable	312:317	arg1	SAHA					370:373	SAHA	370:373	SAHA	370:373	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	2	36	theme	stable	312:317	arg1	aerogel					361:367	low-cost, and stable sodium alginate-halloysite clay composite aerogel	298:367	low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA)	298:374	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	2	36	theme	stable	312:317	arg1	friendly					288:295	an environmentally friendly	269:295	an environmentally friendly	269:295	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	1	37	theme	various	170:176	arg1	issues					196:201	various pollution-related issues	170:201	various pollution-related issues	170:201	Environmental remediation using green approaches for addressing various pollution-related issues, especially water pollution, is in high demand.
33287322	5	38	theme	minerals	995:1002	arg1	presence					983:990	the presence	979:990	the presence of minerals, such as magnesium, sodium, aluminum, and silicon in the SAHA aerogels	979:1073	The elemental composition of the hydrogels investigated using energy dispersive X-ray spectrometry (EDX) revealed the presence of minerals, such as magnesium, sodium, aluminum, and silicon in the SAHA aerogels.
33287322	3	39	theme	transformation	651:664	arg1	spectroscopy					683:694	Fourier transformation infrared (FT-IR) spectroscopy	643:694	Fourier transformation infrared (FT-IR) spectroscopy	643:694	The as-prepared SAHA aerogels were characterized in detail by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transformation infrared (FT-IR) spectroscopy.
33287322	7	40	theme	interesting	1404:1414	arg1	candidate					1416:1424	an interesting candidate	1401:1424	an interesting candidate for practical applications in water recycling	1401:1470	The ease of fabrication, excellent oil/water separation, and multiple performances make the SAHA aerogel an interesting candidate for practical applications in water recycling.
33287322	1	41	theme	high	238:241	arg1	demand					243:248	high demand	238:248	high demand	238:248	Environmental remediation using green approaches for addressing various pollution-related issues, especially water pollution, is in high demand.
33287322	4	42	with	microstructure	771:784	arg1	HA					833:834	HA	833:834	HA	833:834	Characterization of the SAHA aerogels revealed a three-dimensional porous microstructure with uniformly dispersed halloysite nanotubes (HA) within the alginate matrix.
33287322	4	42	with	microstructure	771:784	arg1	nanotubes					822:830	uniformly dispersed halloysite nanotubes	791:830	uniformly dispersed halloysite nanotubes (HA) within the alginate matrix	791:862	Characterization of the SAHA aerogels revealed a three-dimensional porous microstructure with uniformly dispersed halloysite nanotubes (HA) within the alginate matrix.
33287322	3	43	theme	X-ray	614:618	arg1	XRD					633:635	XRD	633:635	XRD	633:635	The as-prepared SAHA aerogels were characterized in detail by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transformation infrared (FT-IR) spectroscopy.
33287322	3	43	theme	X-ray	614:618	arg1	diffraction					620:630	X-ray diffraction	614:630	X-ray diffraction (XRD)	614:636	The as-prepared SAHA aerogels were characterized in detail by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transformation infrared (FT-IR) spectroscopy.
33287322	3	44	theme	as-prepared	489:499	arg1	aerogels					506:513	The as-prepared SAHA aerogels	485:513	The as-prepared SAHA aerogels	485:513	The as-prepared SAHA aerogels were characterized in detail by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transformation infrared (FT-IR) spectroscopy.
33287322	6	45	theme	matrix	1115:1120	arg1	presence					1080:1087	The presence	1076:1087	The presence of a hydrophilic alginate matrix combined with these unique morphological characteristics	1076:1177	The presence of a hydrophilic alginate matrix combined with these unique morphological characteristics resulted in SAHA aerogels with underwater oleophobicity and excellent oil/water separation efficiency (up to 99.7%).
33287322	1	46	theme	green	138:142	arg1	pollution					221:229	water pollution	215:229	especially water pollution	204:229	Environmental remediation using green approaches for addressing various pollution-related issues, especially water pollution, is in high demand.
33287322	1	46	theme	green	138:142	arg1	approaches					144:153	green approaches	138:153	green approaches for addressing various pollution-related issues	138:201	Environmental remediation using green approaches for addressing various pollution-related issues, especially water pollution, is in high demand.
33287322	3	47	theme	FT-IR	676:680	arg1	spectroscopy					683:694	Fourier transformation infrared (FT-IR) spectroscopy	643:694	Fourier transformation infrared (FT-IR) spectroscopy	643:694	The as-prepared SAHA aerogels were characterized in detail by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transformation infrared (FT-IR) spectroscopy.
33287322	1	48	theme	pollution-related	178:194	arg1	issues					196:201	various pollution-related issues	170:201	various pollution-related issues	170:201	Environmental remediation using green approaches for addressing various pollution-related issues, especially water pollution, is in high demand.
33287322	7	49	theme	multiple	1357:1364	arg1	performances					1366:1377	multiple performances	1357:1377	multiple performances	1357:1377	The ease of fabrication, excellent oil/water separation, and multiple performances make the SAHA aerogel an interesting candidate for practical applications in water recycling.
33287322	5	50	from	presence	983:990	arg1	aerogels					1066:1073	the SAHA aerogels	1057:1073	the SAHA aerogels	1057:1073	The elemental composition of the hydrogels investigated using energy dispersive X-ray spectrometry (EDX) revealed the presence of minerals, such as magnesium, sodium, aluminum, and silicon in the SAHA aerogels.
33287322	5	51	attach	presence	983:990	arg2	silicon					1046:1052	silicon	1046:1052	silicon	1046:1052	The elemental composition of the hydrogels investigated using energy dispersive X-ray spectrometry (EDX) revealed the presence of minerals, such as magnesium, sodium, aluminum, and silicon in the SAHA aerogels.
33287322	5	51	attach	presence	983:990	arg1	aerogels					1066:1073	the SAHA aerogels	1057:1073	the SAHA aerogels	1057:1073	The elemental composition of the hydrogels investigated using energy dispersive X-ray spectrometry (EDX) revealed the presence of minerals, such as magnesium, sodium, aluminum, and silicon in the SAHA aerogels.
33287322	5	51	attach	presence	983:990	arg2	magnesium					1013:1021	magnesium	1013:1021	magnesium	1013:1021	The elemental composition of the hydrogels investigated using energy dispersive X-ray spectrometry (EDX) revealed the presence of minerals, such as magnesium, sodium, aluminum, and silicon in the SAHA aerogels.
33287322	5	51	attach	presence	983:990	arg2	minerals					995:1002	minerals	995:1002	minerals	995:1002	The elemental composition of the hydrogels investigated using energy dispersive X-ray spectrometry (EDX) revealed the presence of minerals, such as magnesium, sodium, aluminum, and silicon in the SAHA aerogels.
33287322	5	51	attach	presence	983:990	arg2	sodium					1024:1029	sodium	1024:1029	sodium	1024:1029	The elemental composition of the hydrogels investigated using energy dispersive X-ray spectrometry (EDX) revealed the presence of minerals, such as magnesium, sodium, aluminum, and silicon in the SAHA aerogels.
33287322	5	51	attach	presence	983:990	arg2	aluminum					1032:1039	aluminum	1032:1039	aluminum	1032:1039	The elemental composition of the hydrogels investigated using energy dispersive X-ray spectrometry (EDX) revealed the presence of minerals, such as magnesium, sodium, aluminum, and silicon in the SAHA aerogels.
33287322	6	52	theme	oil/water	1249:1257	arg1	%					1292:1292	up to 99.7%	1282:1292	up to 99.7%	1282:1292	The presence of a hydrophilic alginate matrix combined with these unique morphological characteristics resulted in SAHA aerogels with underwater oleophobicity and excellent oil/water separation efficiency (up to 99.7%).
33287322	6	52	theme	oil/water	1249:1257	arg1	efficiency					1270:1279	excellent oil/water separation efficiency	1239:1279	excellent oil/water separation efficiency (up to 99.7%)	1239:1293	The presence of a hydrophilic alginate matrix combined with these unique morphological characteristics resulted in SAHA aerogels with underwater oleophobicity and excellent oil/water separation efficiency (up to 99.7%).
33287322	7	53	theme	water	1456:1460	arg1	recycling					1462:1470	water recycling	1456:1470	water recycling	1456:1470	The ease of fabrication, excellent oil/water separation, and multiple performances make the SAHA aerogel an interesting candidate for practical applications in water recycling.
33287322	3	54	theme	atomic	583:588	arg1	AFM					608:610	AFM	608:610	AFM	608:610	The as-prepared SAHA aerogels were characterized in detail by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transformation infrared (FT-IR) spectroscopy.
33287322	3	54	theme	atomic	583:588	arg1	microscopy					596:605	atomic force microscopy	583:605	atomic force microscopy (AFM)	583:611	The as-prepared SAHA aerogels were characterized in detail by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transformation infrared (FT-IR) spectroscopy.
33287322	7	55	theme	practical	1430:1438	arg1	applications					1440:1451	practical applications	1430:1451	practical applications in water recycling	1430:1470	The ease of fabrication, excellent oil/water separation, and multiple performances make the SAHA aerogel an interesting candidate for practical applications in water recycling.
33287322	6	56	theme	SAHA	1191:1194	arg1	aerogels					1196:1203	SAHA aerogels	1191:1203	SAHA aerogels with underwater oleophobicity and excellent oil/water separation efficiency (up to 99.7%)	1191:1293	The presence of a hydrophilic alginate matrix combined with these unique morphological characteristics resulted in SAHA aerogels with underwater oleophobicity and excellent oil/water separation efficiency (up to 99.7%).
33287322	3	57	theme	infrared	666:673	arg1	spectroscopy					683:694	Fourier transformation infrared (FT-IR) spectroscopy	643:694	Fourier transformation infrared (FT-IR) spectroscopy	643:694	The as-prepared SAHA aerogels were characterized in detail by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transformation infrared (FT-IR) spectroscopy.
33287322	0	58	dep	Aerogel	34:40	arg1	Alginate-Halloysite					0:18	Alginate-Halloysite	0:18	Alginate-Halloysite	0:18	Alginate-Halloysite Nanocomposite Aerogel: Preparation, Structure, and Oil/Water Separation Applications.
33287322	0	58	dep	Aerogel	34:40	arg1	Applications					92:103	Oil/Water Separation Applications	71:103	Oil/Water Separation Applications	71:103	Alginate-Halloysite Nanocomposite Aerogel: Preparation, Structure, and Oil/Water Separation Applications.
33287322	0	58	dep	Aerogel	34:40	arg1	Structure					56:64	Structure	56:64	Structure	56:64	Alginate-Halloysite Nanocomposite Aerogel: Preparation, Structure, and Oil/Water Separation Applications.
33287322	0	58	dep	Aerogel	34:40	arg1	Preparation					43:53	Preparation	43:53	Preparation	43:53	Alginate-Halloysite Nanocomposite Aerogel: Preparation, Structure, and Oil/Water Separation Applications.
33287322	4	59	theme	porous	764:769	arg1	microstructure					771:784	a three-dimensional porous microstructure	744:784	a three-dimensional porous microstructure with uniformly dispersed halloysite nanotubes (HA) within the alginate matrix	744:862	Characterization of the SAHA aerogels revealed a three-dimensional porous microstructure with uniformly dispersed halloysite nanotubes (HA) within the alginate matrix.
33287322	2	60	theme	composite	351:359	arg1	SAHA					370:373	SAHA	370:373	SAHA	370:373	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	2	60	theme	composite	351:359	arg1	aerogel					361:367	low-cost, and stable sodium alginate-halloysite clay composite aerogel	298:367	low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA)	298:374	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	2	60	theme	composite	351:359	arg1	friendly					288:295	an environmentally friendly	269:295	an environmentally friendly	269:295	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	6	61	theme	unique	1142:1147	arg1	characteristics					1163:1177	these unique morphological characteristics	1136:1177	these unique morphological characteristics	1136:1177	The presence of a hydrophilic alginate matrix combined with these unique morphological characteristics resulted in SAHA aerogels with underwater oleophobicity and excellent oil/water separation efficiency (up to 99.7%).
33287322	0	62	theme	Oil/Water	71:79	arg1	Applications					92:103	Oil/Water Separation Applications	71:103	Oil/Water Separation Applications	71:103	Alginate-Halloysite Nanocomposite Aerogel: Preparation, Structure, and Oil/Water Separation Applications.
33287322	6	63	theme	alginate	1106:1113	arg1	matrix					1115:1120	a hydrophilic alginate matrix	1092:1120	a hydrophilic alginate matrix combined with these unique morphological characteristics	1092:1177	The presence of a hydrophilic alginate matrix combined with these unique morphological characteristics resulted in SAHA aerogels with underwater oleophobicity and excellent oil/water separation efficiency (up to 99.7%).
33287322	7	64	theme	performances	1366:1377	arg1	ease					1300:1303	The ease	1296:1303	The ease of fabrication, excellent oil/water separation, and multiple performances	1296:1377	The ease of fabrication, excellent oil/water separation, and multiple performances make the SAHA aerogel an interesting candidate for practical applications in water recycling.
33287322	3	65	theme	SAHA	501:504	arg1	aerogels					506:513	The as-prepared SAHA aerogels	485:513	The as-prepared SAHA aerogels	485:513	The as-prepared SAHA aerogels were characterized in detail by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transformation infrared (FT-IR) spectroscopy.
33287322	3	66	theme	electron	556:563	arg1	SEM					577:579	SEM	577:579	SEM	577:579	The as-prepared SAHA aerogels were characterized in detail by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transformation infrared (FT-IR) spectroscopy.
33287322	3	66	theme	electron	556:563	arg1	microscopy					565:574	scanning electron microscopy	547:574	scanning electron microscopy (SEM)	547:580	The as-prepared SAHA aerogels were characterized in detail by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transformation infrared (FT-IR) spectroscopy.
33287322	2	67	theme	two-step	407:414	arg1	procedure					426:434	a two-step synthesis procedure	405:434	a two-step synthesis procedure	405:434	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
33287322	6	68	theme	morphological	1149:1161	arg1	characteristics					1163:1177	these unique morphological characteristics	1136:1177	these unique morphological characteristics	1136:1177	The presence of a hydrophilic alginate matrix combined with these unique morphological characteristics resulted in SAHA aerogels with underwater oleophobicity and excellent oil/water separation efficiency (up to 99.7%).
33287322	6	69	theme	separation	1259:1268	arg1	%					1292:1292	up to 99.7%	1282:1292	up to 99.7%	1282:1292	The presence of a hydrophilic alginate matrix combined with these unique morphological characteristics resulted in SAHA aerogels with underwater oleophobicity and excellent oil/water separation efficiency (up to 99.7%).
33287322	6	69	theme	separation	1259:1268	arg1	efficiency					1270:1279	excellent oil/water separation efficiency	1239:1279	excellent oil/water separation efficiency (up to 99.7%)	1239:1293	The presence of a hydrophilic alginate matrix combined with these unique morphological characteristics resulted in SAHA aerogels with underwater oleophobicity and excellent oil/water separation efficiency (up to 99.7%).
33287322	2	70	theme	oil/water	380:388	arg1	separation					390:399	oil/water separation	380:399	oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying	380:482	Here, we designed an environmentally friendly, low-cost, and stable sodium alginate-halloysite clay composite aerogel (SAHA) for oil/water separation via a two-step synthesis procedure, including ionic crosslinking and freeze-drying.
32678191	2	0	theme	P.	345:346	arg1	seeds					354:358	mature P. ovata seeds	338:358	mature P. ovata seeds	338:358	We have used microscopy techniques to track the development of mucilage secretory cells and demonstrate that mature P. ovata seeds do not have an outer intact cell layer within which the polysaccharides surround internal columellae.
32678191	7	1	theme	processes	1359:1367	arg1	many					1345:1348	many	1345:1348	many	1345:1348	Our results indicate that the synthesis, deposition, desiccation, and final storage position of mucilage polysaccharides must be carefully orchestrated, although many of these processes are not yet fully defined and vary widely between myxospermous plant species.
32678191	7	1	theme	processes	1359:1367	arg1	processes					1359:1367	these processes	1353:1367	these processes	1353:1367	Our results indicate that the synthesis, deposition, desiccation, and final storage position of mucilage polysaccharides must be carefully orchestrated, although many of these processes are not yet fully defined and vary widely between myxospermous plant species.
32678191	5	2	from	rich	875:878	arg1	pectin					883:888	pectin	883:888	pectin	883:888	The first emergent layer of hydrated mucilage is rich in pectin, extremely hydrophilic, and forms an expansion front that functions to 'jumpstart' hydration and swelling of the second layer.
32678191	2	3	theme	mature	338:343	arg1	seeds					354:358	mature P. ovata seeds	338:358	mature P. ovata seeds	338:358	We have used microscopy techniques to track the development of mucilage secretory cells and demonstrate that mature P. ovata seeds do not have an outer intact cell layer within which the polysaccharides surround internal columellae.
32678191	7	4	theme	myxospermous	1419:1430	arg1	species					1438:1444	myxospermous plant species	1419:1444	myxospermous plant species	1419:1444	Our results indicate that the synthesis, deposition, desiccation, and final storage position of mucilage polysaccharides must be carefully orchestrated, although many of these processes are not yet fully defined and vary widely between myxospermous plant species.
32678191	2	5	dep	P.	345:346	arg1	ovata					348:352	ovata	348:352	ovata	348:352	We have used microscopy techniques to track the development of mucilage secretory cells and demonstrate that mature P. ovata seeds do not have an outer intact cell layer within which the polysaccharides surround internal columellae.
32678191	3	6	theme	entire	537:542	arg1	surface					549:555	the entire seed surface	533:555	the entire seed surface	533:555	Instead, dehydrated mucilage is spread in a thin homogenous layer over the entire seed surface and upon wetting expands directly outwards, away from the seed.
32678191	3	7	theme	seed	544:547	arg1	surface					549:555	the entire seed surface	533:555	the entire seed surface	533:555	Instead, dehydrated mucilage is spread in a thin homogenous layer over the entire seed surface and upon wetting expands directly outwards, away from the seed.
32678191	5	8	theme	hydrophilic	901:911	arg1	pectin					883:888	pectin	883:888	pectin	883:888	The first emergent layer of hydrated mucilage is rich in pectin, extremely hydrophilic, and forms an expansion front that functions to 'jumpstart' hydration and swelling of the second layer.
32678191	7	9	dep	synthesis	1213:1221	arg1	the					1209:1211	the	1209:1211	the	1209:1211	Our results indicate that the synthesis, deposition, desiccation, and final storage position of mucilage polysaccharides must be carefully orchestrated, although many of these processes are not yet fully defined and vary widely between myxospermous plant species.
32678191	5	10	theme	emergent	836:843	arg1	rich					875:878	rich	875:878	rich	875:878	The first emergent layer of hydrated mucilage is rich in pectin, extremely hydrophilic, and forms an expansion front that functions to 'jumpstart' hydration and swelling of the second layer.
32678191	5	10	theme	emergent	836:843	arg1	layer					845:849	The first emergent layer	826:849	The first emergent layer of hydrated mucilage	826:870	The first emergent layer of hydrated mucilage is rich in pectin, extremely hydrophilic, and forms an expansion front that functions to 'jumpstart' hydration and swelling of the second layer.
32678191	3	11	theme	dehydrated	471:480	arg1	mucilage					482:489	dehydrated mucilage	471:489	dehydrated mucilage	471:489	Instead, dehydrated mucilage is spread in a thin homogenous layer over the entire seed surface and upon wetting expands directly outwards, away from the seed.
32678191	1	12	from	release	139:145	arg1	ovata					159:163	ovata	159:163	ovata	159:163	Seed mucilage polysaccharide production, storage and release in Plantago ovata is strikingly different to that of the model plant Arabidopsis.
32678191	3	13	theme	thin	506:509	arg1	layer					522:526	a thin homogenous layer	504:526	a thin homogenous layer	504:526	Instead, dehydrated mucilage is spread in a thin homogenous layer over the entire seed surface and upon wetting expands directly outwards, away from the seed.
32678191	4	14	from	expansion	640:648	arg1	time					658:661	real time	653:661	real time combined with compositional analysis	653:698	Observing mucilage expansion in real time combined with compositional analysis allowed mucilage layer definition and the roles they play in mucilage release and architecture upon hydration to be explored.
32678191	4	15	theme	mucilage	631:638	arg1	expansion					640:648	Observing mucilage expansion	621:648	Observing mucilage expansion in real time combined with compositional analysis	621:698	Observing mucilage expansion in real time combined with compositional analysis allowed mucilage layer definition and the roles they play in mucilage release and architecture upon hydration to be explored.
32678191	6	16	theme	expanded	1061:1068	arg1	mucilage					1075:1082	the expanded seed mucilage	1057:1082	the expanded seed mucilage	1057:1082	This next layer, comprising the bulk of the expanded seed mucilage, is predominantly composed of heteroxylan and appears to provide much of the structural integrity.
32678191	3	17	theme	homogenous	511:520	arg1	layer					522:526	a thin homogenous layer	504:526	a thin homogenous layer	504:526	Instead, dehydrated mucilage is spread in a thin homogenous layer over the entire seed surface and upon wetting expands directly outwards, away from the seed.
32678191	2	18	used	used	237:240	arg2	We					229:230	We	229:230	We	229:230	We have used microscopy techniques to track the development of mucilage secretory cells and demonstrate that mature P. ovata seeds do not have an outer intact cell layer within which the polysaccharides surround internal columellae.
32678191	4	19	theme	Observing	621:629	arg1	expansion					640:648	Observing mucilage expansion	621:648	Observing mucilage expansion in real time combined with compositional analysis	621:698	Observing mucilage expansion in real time combined with compositional analysis allowed mucilage layer definition and the roles they play in mucilage release and architecture upon hydration to be explored.
32678191	0	20	theme	novel	4:8	arg1	features					10:17	The novel features	0:17	The novel features of Plantago ovata	0:35	The novel features of Plantago ovata seed mucilage accumulation, storage and release.
32678191	1	21	from	storage	127:133	arg1	ovata					159:163	ovata	159:163	ovata	159:163	Seed mucilage polysaccharide production, storage and release in Plantago ovata is strikingly different to that of the model plant Arabidopsis.
32678191	2	22	theme	secretory	301:309	arg1	cells					311:315	mucilage secretory cells	292:315	mucilage secretory cells	292:315	We have used microscopy techniques to track the development of mucilage secretory cells and demonstrate that mature P. ovata seeds do not have an outer intact cell layer within which the polysaccharides surround internal columellae.
32678191	6	23	theme	next	1022:1025	arg1	layer					1027:1031	This next layer	1017:1031	This next layer	1017:1031	This next layer, comprising the bulk of the expanded seed mucilage, is predominantly composed of heteroxylan and appears to provide much of the structural integrity.
32678191	7	24	theme	final	1253:1257	arg1	position					1267:1274	final storage position	1253:1274	final storage position	1253:1274	Our results indicate that the synthesis, deposition, desiccation, and final storage position of mucilage polysaccharides must be carefully orchestrated, although many of these processes are not yet fully defined and vary widely between myxospermous plant species.
32678191	5	25	from	pectin	883:888	arg1	rich					875:878	rich	875:878	rich	875:878	The first emergent layer of hydrated mucilage is rich in pectin, extremely hydrophilic, and forms an expansion front that functions to 'jumpstart' hydration and swelling of the second layer.
32678191	5	25	from	pectin	883:888	arg1	layer					845:849	The first emergent layer	826:849	The first emergent layer of hydrated mucilage	826:870	The first emergent layer of hydrated mucilage is rich in pectin, extremely hydrophilic, and forms an expansion front that functions to 'jumpstart' hydration and swelling of the second layer.
32678191	2	26	theme	mucilage	292:299	arg1	cells					311:315	mucilage secretory cells	292:315	mucilage secretory cells	292:315	We have used microscopy techniques to track the development of mucilage secretory cells and demonstrate that mature P. ovata seeds do not have an outer intact cell layer within which the polysaccharides surround internal columellae.
32678191	5	27	theme	hydrated	854:861	arg1	mucilage					863:870	hydrated mucilage	854:870	hydrated mucilage	854:870	The first emergent layer of hydrated mucilage is rich in pectin, extremely hydrophilic, and forms an expansion front that functions to 'jumpstart' hydration and swelling of the second layer.
32678191	4	28	theme	layer	717:721	arg1	definition					723:732	mucilage layer definition	708:732	mucilage layer definition	708:732	Observing mucilage expansion in real time combined with compositional analysis allowed mucilage layer definition and the roles they play in mucilage release and architecture upon hydration to be explored.
32678191	1	29	from	production	115:124	arg1	ovata					159:163	ovata	159:163	ovata	159:163	Seed mucilage polysaccharide production, storage and release in Plantago ovata is strikingly different to that of the model plant Arabidopsis.
32678191	1	30	theme	Seed	86:89	arg1	production					115:124	Seed mucilage polysaccharide production	86:124	Seed mucilage polysaccharide production	86:124	Seed mucilage polysaccharide production, storage and release in Plantago ovata is strikingly different to that of the model plant Arabidopsis.
32678191	5	31	theme	expansion	927:935	arg1	front					937:941	an expansion front	924:941	an expansion front that functions to 'jumpstart' hydration and swelling of the second layer	924:1014	The first emergent layer of hydrated mucilage is rich in pectin, extremely hydrophilic, and forms an expansion front that functions to 'jumpstart' hydration and swelling of the second layer.
32678191	3	32	dep	spread	494:499	arg1	outwards					591:598	outwards	591:598	outwards	591:598	Instead, dehydrated mucilage is spread in a thin homogenous layer over the entire seed surface and upon wetting expands directly outwards, away from the seed.
32678191	1	33	theme	mucilage	91:98	arg1	production					115:124	Seed mucilage polysaccharide production	86:124	Seed mucilage polysaccharide production	86:124	Seed mucilage polysaccharide production, storage and release in Plantago ovata is strikingly different to that of the model plant Arabidopsis.
32678191	2	34	theme	cell	388:391	arg1	layer					393:397	an outer intact cell layer	372:397	an outer intact cell layer within which the polysaccharides surround internal columellae	372:459	We have used microscopy techniques to track the development of mucilage secretory cells and demonstrate that mature P. ovata seeds do not have an outer intact cell layer within which the polysaccharides surround internal columellae.
32678191	0	35	theme	ovata	31:35	arg1	features					10:17	The novel features	0:17	The novel features of Plantago ovata	0:35	The novel features of Plantago ovata seed mucilage accumulation, storage and release.
32678191	6	36	theme	seed	1070:1073	arg1	mucilage					1075:1082	the expanded seed mucilage	1057:1082	the expanded seed mucilage	1057:1082	This next layer, comprising the bulk of the expanded seed mucilage, is predominantly composed of heteroxylan and appears to provide much of the structural integrity.
32678191	3	37	theme	wetting	566:572	arg1	expands					574:580	wetting expands	566:580	wetting expands	566:580	Instead, dehydrated mucilage is spread in a thin homogenous layer over the entire seed surface and upon wetting expands directly outwards, away from the seed.
32678191	7	38	theme	storage	1259:1265	arg1	position					1267:1274	final storage position	1253:1274	final storage position	1253:1274	Our results indicate that the synthesis, deposition, desiccation, and final storage position of mucilage polysaccharides must be carefully orchestrated, although many of these processes are not yet fully defined and vary widely between myxospermous plant species.
32678191	2	39	theme	intact	381:386	arg1	layer					393:397	an outer intact cell layer	372:397	an outer intact cell layer within which the polysaccharides surround internal columellae	372:459	We have used microscopy techniques to track the development of mucilage secretory cells and demonstrate that mature P. ovata seeds do not have an outer intact cell layer within which the polysaccharides surround internal columellae.
32678191	0	40	theme	Plantago	22:29	arg1	ovata					31:35	Plantago ovata	22:35	Plantago ovata	22:35	The novel features of Plantago ovata seed mucilage accumulation, storage and release.
32678191	2	41	contain	have	367:370	arg2	layer					393:397	an outer intact cell layer	372:397	an outer intact cell layer within which the polysaccharides surround internal columellae	372:459	We have used microscopy techniques to track the development of mucilage secretory cells and demonstrate that mature P. ovata seeds do not have an outer intact cell layer within which the polysaccharides surround internal columellae.
32678191	2	41	contain	have	367:370	arg1	seeds					354:358	mature P. ovata seeds	338:358	mature P. ovata seeds	338:358	We have used microscopy techniques to track the development of mucilage secretory cells and demonstrate that mature P. ovata seeds do not have an outer intact cell layer within which the polysaccharides surround internal columellae.
32678191	6	42	theme	integrity	1172:1180	arg1	much					1149:1152	much	1149:1152	much	1149:1152	This next layer, comprising the bulk of the expanded seed mucilage, is predominantly composed of heteroxylan and appears to provide much of the structural integrity.
32678191	2	43	theme	outer	375:379	arg1	layer					393:397	an outer intact cell layer	372:397	an outer intact cell layer within which the polysaccharides surround internal columellae	372:459	We have used microscopy techniques to track the development of mucilage secretory cells and demonstrate that mature P. ovata seeds do not have an outer intact cell layer within which the polysaccharides surround internal columellae.
32678191	0	44	theme	mucilage	42:49	arg1	accumulation					51:62	mucilage accumulation	42:62	mucilage accumulation	42:62	The novel features of Plantago ovata seed mucilage accumulation, storage and release.
32678191	6	45	theme	structural	1161:1170	arg1	integrity					1172:1180	the structural integrity	1157:1180	the structural integrity	1157:1180	This next layer, comprising the bulk of the expanded seed mucilage, is predominantly composed of heteroxylan and appears to provide much of the structural integrity.
32678191	1	46	theme	polysaccharide	100:113	arg1	production					115:124	Seed mucilage polysaccharide production	86:124	Seed mucilage polysaccharide production	86:124	Seed mucilage polysaccharide production, storage and release in Plantago ovata is strikingly different to that of the model plant Arabidopsis.
32678191	4	47	theme	compositional	677:689	arg1	analysis					691:698	compositional analysis	677:698	compositional analysis	677:698	Observing mucilage expansion in real time combined with compositional analysis allowed mucilage layer definition and the roles they play in mucilage release and architecture upon hydration to be explored.
32678191	4	48	dep	roles	742:746	arg1	play					753:756	play	753:756	play in mucilage release and architecture upon hydration to be explored	753:823	Observing mucilage expansion in real time combined with compositional analysis allowed mucilage layer definition and the roles they play in mucilage release and architecture upon hydration to be explored.
32678191	5	49	theme	second	1003:1008	arg1	layer					1010:1014	the second layer	999:1014	the second layer	999:1014	The first emergent layer of hydrated mucilage is rich in pectin, extremely hydrophilic, and forms an expansion front that functions to 'jumpstart' hydration and swelling of the second layer.
32678191	2	50	theme	microscopy	242:251	arg1	techniques					253:262	microscopy techniques	242:262	microscopy techniques	242:262	We have used microscopy techniques to track the development of mucilage secretory cells and demonstrate that mature P. ovata seeds do not have an outer intact cell layer within which the polysaccharides surround internal columellae.
32678191	4	51	theme	mucilage	708:715	arg1	definition					723:732	mucilage layer definition	708:732	mucilage layer definition	708:732	Observing mucilage expansion in real time combined with compositional analysis allowed mucilage layer definition and the roles they play in mucilage release and architecture upon hydration to be explored.
32678191	1	52	theme	model	204:208	arg1	Arabidopsis					216:226	the model plant Arabidopsis	200:226	the model plant Arabidopsis	200:226	Seed mucilage polysaccharide production, storage and release in Plantago ovata is strikingly different to that of the model plant Arabidopsis.
32678191	5	53	theme	layer	1010:1014	arg1	jumpstart					962:970	'jumpstart' hydration and swelling	961:994	'jumpstart' hydration and swelling of the second layer	961:1014	The first emergent layer of hydrated mucilage is rich in pectin, extremely hydrophilic, and forms an expansion front that functions to 'jumpstart' hydration and swelling of the second layer.
32678191	4	54	theme	mucilage	761:768	arg1	release					770:776	mucilage release	761:776	mucilage release	761:776	Observing mucilage expansion in real time combined with compositional analysis allowed mucilage layer definition and the roles they play in mucilage release and architecture upon hydration to be explored.
32678191	2	55	theme	cells	311:315	arg1	development					277:287	the development	273:287	the development of mucilage secretory cells	273:315	We have used microscopy techniques to track the development of mucilage secretory cells and demonstrate that mature P. ovata seeds do not have an outer intact cell layer within which the polysaccharides surround internal columellae.
32678191	6	56	theme	mucilage	1075:1082	arg1	bulk					1049:1052	the bulk	1045:1052	the bulk of the expanded seed mucilage	1045:1082	This next layer, comprising the bulk of the expanded seed mucilage, is predominantly composed of heteroxylan and appears to provide much of the structural integrity.
32678191	1	57	theme	plant	210:214	arg1	Arabidopsis					216:226	the model plant Arabidopsis	200:226	the model plant Arabidopsis	200:226	Seed mucilage polysaccharide production, storage and release in Plantago ovata is strikingly different to that of the model plant Arabidopsis.
32678191	5	58	theme	first	830:834	arg1	rich					875:878	rich	875:878	rich	875:878	The first emergent layer of hydrated mucilage is rich in pectin, extremely hydrophilic, and forms an expansion front that functions to 'jumpstart' hydration and swelling of the second layer.
32678191	5	58	theme	first	830:834	arg1	layer					845:849	The first emergent layer	826:849	The first emergent layer of hydrated mucilage	826:870	The first emergent layer of hydrated mucilage is rich in pectin, extremely hydrophilic, and forms an expansion front that functions to 'jumpstart' hydration and swelling of the second layer.
32678191	7	59	theme	mucilage	1279:1286	arg1	polysaccharides					1288:1302	mucilage polysaccharides	1279:1302	mucilage polysaccharides	1279:1302	Our results indicate that the synthesis, deposition, desiccation, and final storage position of mucilage polysaccharides must be carefully orchestrated, although many of these processes are not yet fully defined and vary widely between myxospermous plant species.
32678191	5	60	dep	jumpstart	962:970	arg1	swelling					987:994	swelling	987:994	swelling	987:994	The first emergent layer of hydrated mucilage is rich in pectin, extremely hydrophilic, and forms an expansion front that functions to 'jumpstart' hydration and swelling of the second layer.
32678191	5	60	dep	jumpstart	962:970	arg1	hydration					973:981	hydration	973:981	hydration	973:981	The first emergent layer of hydrated mucilage is rich in pectin, extremely hydrophilic, and forms an expansion front that functions to 'jumpstart' hydration and swelling of the second layer.
32678191	5	61	theme	mucilage	863:870	arg1	rich					875:878	rich	875:878	rich	875:878	The first emergent layer of hydrated mucilage is rich in pectin, extremely hydrophilic, and forms an expansion front that functions to 'jumpstart' hydration and swelling of the second layer.
32678191	5	61	theme	mucilage	863:870	arg1	layer					845:849	The first emergent layer	826:849	The first emergent layer of hydrated mucilage	826:870	The first emergent layer of hydrated mucilage is rich in pectin, extremely hydrophilic, and forms an expansion front that functions to 'jumpstart' hydration and swelling of the second layer.
32678191	2	62	theme	internal	441:448	arg1	columellae					450:459	internal columellae	441:459	internal columellae	441:459	We have used microscopy techniques to track the development of mucilage secretory cells and demonstrate that mature P. ovata seeds do not have an outer intact cell layer within which the polysaccharides surround internal columellae.
32678191	7	63	theme	plant	1432:1436	arg1	species					1438:1444	myxospermous plant species	1419:1444	myxospermous plant species	1419:1444	Our results indicate that the synthesis, deposition, desiccation, and final storage position of mucilage polysaccharides must be carefully orchestrated, although many of these processes are not yet fully defined and vary widely between myxospermous plant species.
32678191	7	64	theme	polysaccharides	1288:1302	arg1	synthesis					1213:1221	synthesis	1213:1221	synthesis	1213:1221	Our results indicate that the synthesis, deposition, desiccation, and final storage position of mucilage polysaccharides must be carefully orchestrated, although many of these processes are not yet fully defined and vary widely between myxospermous plant species.
32678191	7	64	theme	polysaccharides	1288:1302	arg1	position					1267:1274	final storage position	1253:1274	final storage position	1253:1274	Our results indicate that the synthesis, deposition, desiccation, and final storage position of mucilage polysaccharides must be carefully orchestrated, although many of these processes are not yet fully defined and vary widely between myxospermous plant species.
32678191	7	64	theme	polysaccharides	1288:1302	arg1	desiccation					1236:1246	desiccation	1236:1246	desiccation	1236:1246	Our results indicate that the synthesis, deposition, desiccation, and final storage position of mucilage polysaccharides must be carefully orchestrated, although many of these processes are not yet fully defined and vary widely between myxospermous plant species.
32678191	7	64	theme	polysaccharides	1288:1302	arg1	deposition					1224:1233	deposition	1224:1233	deposition	1224:1233	Our results indicate that the synthesis, deposition, desiccation, and final storage position of mucilage polysaccharides must be carefully orchestrated, although many of these processes are not yet fully defined and vary widely between myxospermous plant species.
32678191	4	65	theme	real	653:656	arg1	time					658:661	real time	653:661	real time combined with compositional analysis	653:698	Observing mucilage expansion in real time combined with compositional analysis allowed mucilage layer definition and the roles they play in mucilage release and architecture upon hydration to be explored.
33179485	1	0	from	BACKGROUND	51:60	arg1	production					102:111	sugar production	96:111	sugar production	96:111	BACKGROUND One of the important processes in sugar production is massecuite boiling.
33179485	8	1	theme	standard	1106:1113	arg1	methods					1115:1121	standard methods	1106:1121	standard methods	1106:1121	All the studies of qualitative and quantitative indicators of products and intermediates were performed using standard methods.
33179485	13	2	theme	lowest	1704:1709	arg1	content					1715:1721	the lowest ash content	1700:1721	the lowest ash content	1700:1721	This sample also had the lowest ash content.
33179485	9	3	theme	particle	1136:1143	arg1	distribution					1150:1161	The particle size distribution	1132:1161	The particle size distribution	1132:1161	RESULTS The particle size distribution was determined and the curves of the differential size distribution of sugar fineness were given.
33179485	15	4	dep	massecuite	2093:2102	arg1	made					2104:2107	made	2104:2107	to massecuite made with icing sugar	2090:2124	The most equal crystals were produced using an "Ester K 01" crystallizing agent and a standard factory suspension in comparison to massecuite made with icing sugar.
33179485	18	5	theme	Ester	2476:2480	arg1	"					2486:2486	the crystallizing agent "Ester K 01"	2451:2486	the crystallizing agent "Ester K 01"	2451:2486	CONCLUSIONS In conclusion, the usage of the crystallizing agent "Ester K 01" in the fully automated proces conditions for producing massecuite would be recommended by us.
33179485	2	6	theme	finished	251:258	arg1	product					260:266	the finished product	247:266	the finished product	247:266	The intensification of this process is possible by reducing the boiling time and thus improving the quality of the finished product.
33179485	19	7	theme	uniform	2601:2607	arg1	composition					2623:2633	more uniform granulometric composition	2596:2633	more uniform granulometric composition of sugar	2596:2642	It will allow more uniform granulometric composition of sugar to be obtained.
33179485	17	8	theme	laboratory	2313:2322	arg1	conditions					2324:2333	laboratory conditions	2313:2333	laboratory conditions	2313:2333	The crystal former "Ester K 01" was studied in production and laboratory conditions, and the crystallizing agents' comparative characteristics were also given.
33179485	3	9	theme	effects	492:498	arg1	"					449:449	the crystal former "Ester K 01"	419:449	the crystal former "Ester K 01"	419:449	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	9	theme	effects	492:498	arg1	types					342:346	different types	332:346	different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product	332:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	9	theme	effects	492:498	arg1	comparison					465:474	a comparison	463:474	a comparison of their direct effects on the quality of the finished product	463:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	9	theme	effects	492:498	arg1	sugar					377:381	powdered sugar	368:381	powdered sugar	368:381	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	9	theme	effects	492:498	arg1	suspension					403:412	a standard factory suspension	384:412	a standard factory suspension	384:412	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	10	from	quality	507:513	arg1	"					449:449	the crystal former "Ester K 01"	419:449	the crystal former "Ester K 01"	419:449	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	10	from	quality	507:513	arg1	types					342:346	different types	332:346	different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product	332:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	10	from	quality	507:513	arg1	comparison					465:474	a comparison	463:474	a comparison of their direct effects on the quality of the finished product	463:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	10	from	quality	507:513	arg1	sugar					377:381	powdered sugar	368:381	powdered sugar	368:381	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	10	from	quality	507:513	arg1	suspension					403:412	a standard factory suspension	384:412	a standard factory suspension	384:412	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	4	11	theme	crystal-forming	572:586	arg1	agents					588:593	crystal-forming agents	572:593	crystal-forming agents	572:593	METHODS All laboratory tests of crystal-forming agents were carried out using syrup taken from sugar factories.
33179485	16	12	theme	crystals	2166:2173	arg1	composition					2145:2155	The granulometric composition	2127:2155	The granulometric composition of sugar crystals obtained with different crystal formers	2127:2213	The granulometric composition of sugar crystals obtained with different crystal formers was also compared in this research.
33179485	5	13	theme	Laser	652:656	arg1	diffractometry					658:671	Laser diffractometry	652:671	Laser diffractometry	652:671	Laser diffractometry was used to determine the particle size (dispersion) of the crystalforming agents.
33179485	5	14	theme	agents	748:753	arg1	dispersion					714:723	dispersion	714:723	dispersion	714:723	Laser diffractometry was used to determine the particle size (dispersion) of the crystalforming agents.
33179485	5	14	theme	agents	748:753	arg1	size					708:711	the particle size	695:711	the particle size (dispersion) of the crystalforming agents	695:753	Laser diffractometry was used to determine the particle size (dispersion) of the crystalforming agents.
33179485	14	15	theme	size	1800:1803	arg1	fixation					1782:1789	fixation	1782:1789	fixation of their size after 5, 10, and 20 minutes of adding various types of crystallizing agent	1782:1878	Microphotographs of the sugar crystals were provided with fixation of their size after 5, 10, and 20 minutes of adding various types of crystallizing agent, and the morphology of the sugar crystals in the finished massecuite was studied.
33179485	7	16	theme	vacuum	977:982	arg1	filtration					984:993	a laboratory vacuum filtration	964:993	a laboratory vacuum filtration	964:993	Sugar was obtained by separating the massecuite in a laboratory vacuum filtration.
33179485	9	17	theme	differential	1200:1211	arg1	distribution					1218:1229	the differential size distribution	1196:1229	the differential size distribution of sugar fineness	1196:1247	RESULTS The particle size distribution was determined and the curves of the differential size distribution of sugar fineness were given.
33179485	9	18	theme	size	1213:1216	arg1	distribution					1218:1229	the differential size distribution	1196:1229	the differential size distribution of sugar fineness	1196:1247	RESULTS The particle size distribution was determined and the curves of the differential size distribution of sugar fineness were given.
33179485	12	19	theme	Ester	1646:1650	arg1	"					1656:1656	the "Ester K 01"	1641:1656	the "Ester K 01" crystallizing agent	1641:1676	The highest sucrose content was observed in the sugar sample obtained using the "Ester K 01" crystallizing agent.
33179485	6	20	theme	vacuum	848:853	arg1	evaporator					855:864	a laboratory vacuum evaporator	835:864	a laboratory vacuum evaporator in conditions maximally adapted to production	835:910	Prepared syrup samples were used to obtain the massecuite, which was boiled in a laboratory vacuum evaporator in conditions maximally adapted to production.
33179485	11	21	theme	indicators	1440:1449	arg1	characteristic					1410:1423	a comparative characteristic	1396:1423	a comparative characteristic of the quality indicators	1396:1449	The duration of massecuite boiling was studied and a comparative characteristic of the quality indicators was provided for the obtained massecuite and massecuite syrup in accordance with the type of crystal former used.
33179485	9	22	theme	distribution	1218:1229	arg1	curves					1186:1191	the curves	1182:1191	the curves of the differential size distribution of sugar fineness	1182:1247	RESULTS The particle size distribution was determined and the curves of the differential size distribution of sugar fineness were given.
33179485	12	23	theme	01	1654:1655	arg1	"					1656:1656	the "Ester K 01"	1641:1656	the "Ester K 01" crystallizing agent	1641:1676	The highest sucrose content was observed in the sugar sample obtained using the "Ester K 01" crystallizing agent.
33179485	3	24	theme	boiling	315:321	arg1	methods					293:299	the methods	289:299	the methods of massecuite boiling	289:321	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	15	25	from	agent	2036:2040	arg1	comparison					2079:2088	comparison	2079:2088	comparison	2079:2088	The most equal crystals were produced using an "Ester K 01" crystallizing agent and a standard factory suspension in comparison to massecuite made with icing sugar.
33179485	3	26	from	effects	492:498	arg1	quality					507:513	the quality	503:513	the quality of the finished product	503:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	27	from	sugar	377:381	arg1	quality					507:513	the quality	503:513	the quality of the finished product	503:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	18	28	theme	01	2484:2485	arg1	"					2486:2486	the crystallizing agent "Ester K 01"	2451:2486	the crystallizing agent "Ester K 01"	2451:2486	CONCLUSIONS In conclusion, the usage of the crystallizing agent "Ester K 01" in the fully automated proces conditions for producing massecuite would be recommended by us.
33179485	3	29	from	comparison	465:474	arg1	quality					507:513	the quality	503:513	the quality of the finished product	503:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	5	30	used	used	677:680	arg2	diffractometry					658:671	Laser diffractometry	652:671	Laser diffractometry	652:671	Laser diffractometry was used to determine the particle size (dispersion) of the crystalforming agents.
33179485	14	31	theme	sugar	1748:1752	arg1	crystals					1754:1761	the sugar crystals	1744:1761	the sugar crystals	1744:1761	Microphotographs of the sugar crystals were provided with fixation of their size after 5, 10, and 20 minutes of adding various types of crystallizing agent, and the morphology of the sugar crystals in the finished massecuite was studied.
33179485	0	32	from	Influence	0:8	arg1	quality					42:48	sugar quality	36:48	sugar quality	36:48	Influence of crystallizing agent on sugar quality.
33179485	11	33	theme	obtained	1472:1479	arg1	syrup					1507:1511	the obtained massecuite and massecuite syrup	1468:1511	the obtained massecuite and massecuite syrup in accordance with the type of crystal former used	1468:1562	The duration of massecuite boiling was studied and a comparative characteristic of the quality indicators was provided for the obtained massecuite and massecuite syrup in accordance with the type of crystal former used.
33179485	13	34	contain	had	1696:1698	arg1	sample					1684:1689	This sample	1679:1689	This sample	1679:1689	This sample also had the lowest ash content.
33179485	13	34	contain	had	1696:1698	arg2	content					1715:1721	the lowest ash content	1700:1721	the lowest ash content	1700:1721	This sample also had the lowest ash content.
33179485	14	35	from	crystals	1913:1920	arg1	massecuite					1938:1947	the finished massecuite	1925:1947	the finished massecuite	1925:1947	Microphotographs of the sugar crystals were provided with fixation of their size after 5, 10, and 20 minutes of adding various types of crystallizing agent, and the morphology of the sugar crystals in the finished massecuite was studied.
33179485	14	36	theme	sugar	1907:1911	arg1	crystals					1913:1920	the sugar crystals	1903:1920	the sugar crystals in the finished massecuite	1903:1947	Microphotographs of the sugar crystals were provided with fixation of their size after 5, 10, and 20 minutes of adding various types of crystallizing agent, and the morphology of the sugar crystals in the finished massecuite was studied.
33179485	9	37	dep	RESULTS	1124:1130	arg1	given					1254:1258	given	1254:1258	were given	1249:1258	RESULTS The particle size distribution was determined and the curves of the differential size distribution of sugar fineness were given.
33179485	9	37	dep	RESULTS	1124:1130	arg1	determined					1167:1176	determined	1167:1176	was determined	1163:1176	RESULTS The particle size distribution was determined and the curves of the differential size distribution of sugar fineness were given.
33179485	0	38	theme	sugar	36:40	arg1	quality					42:48	sugar quality	36:48	sugar quality	36:48	Influence of crystallizing agent on sugar quality.
33179485	12	39	theme	sugar	1613:1617	arg1	sample					1619:1624	the sugar sample	1609:1624	the sugar sample obtained using the "Ester K 01" crystallizing agent	1609:1676	The highest sucrose content was observed in the sugar sample obtained using the "Ester K 01" crystallizing agent.
33179485	18	40	theme	K	2482:2482	arg1	"					2486:2486	the crystallizing agent "Ester K 01"	2451:2486	the crystallizing agent "Ester K 01"	2451:2486	CONCLUSIONS In conclusion, the usage of the crystallizing agent "Ester K 01" in the fully automated proces conditions for producing massecuite would be recommended by us.
33179485	11	41	theme	boiling	1372:1378	arg1	duration					1349:1356	The duration	1345:1356	The duration of massecuite boiling	1345:1378	The duration of massecuite boiling was studied and a comparative characteristic of the quality indicators was provided for the obtained massecuite and massecuite syrup in accordance with the type of crystal former used.
33179485	3	42	theme	factory	395:401	arg1	types					342:346	different types	332:346	different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product	332:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	42	theme	factory	395:401	arg1	suspension					403:412	a standard factory suspension	384:412	a standard factory suspension	384:412	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	5	43	theme	crystalforming	733:746	arg1	agents					748:753	the crystalforming agents	729:753	the crystalforming agents	729:753	Laser diffractometry was used to determine the particle size (dispersion) of the crystalforming agents.
33179485	14	44	from	morphology	1889:1898	arg1	massecuite					1938:1947	the finished massecuite	1925:1947	the finished massecuite	1925:1947	Microphotographs of the sugar crystals were provided with fixation of their size after 5, 10, and 20 minutes of adding various types of crystallizing agent, and the morphology of the sugar crystals in the finished massecuite was studied.
33179485	1	45	theme	processes	83:91	arg1	BACKGROUND					51:60	BACKGROUND One	51:64	BACKGROUND One of the important processes in sugar production	51:111	BACKGROUND One of the important processes in sugar production is massecuite boiling.
33179485	12	46	theme	sucrose	1577:1583	arg1	content					1585:1591	The highest sucrose content	1565:1591	The highest sucrose content	1565:1591	The highest sucrose content was observed in the sugar sample obtained using the "Ester K 01" crystallizing agent.
33179485	3	47	theme	crystal	351:357	arg1	formers					359:365	crystal formers	351:365	crystal formers	351:365	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	11	48	theme	massecuite	1481:1490	arg1	syrup					1507:1511	the obtained massecuite and massecuite syrup	1468:1511	the obtained massecuite and massecuite syrup in accordance with the type of crystal former used	1468:1562	The duration of massecuite boiling was studied and a comparative characteristic of the quality indicators was provided for the obtained massecuite and massecuite syrup in accordance with the type of crystal former used.
33179485	3	49	theme	former	431:436	arg1	"					449:449	the crystal former "Ester K 01"	419:449	the crystal former "Ester K 01"	419:449	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	0	50	theme	crystallizing	13:25	arg1	agent					27:31	crystallizing agent	13:31	crystallizing agent	13:31	Influence of crystallizing agent on sugar quality.
33179485	1	51	theme	sugar	96:100	arg1	production					102:111	sugar production	96:111	sugar production	96:111	BACKGROUND One of the important processes in sugar production is massecuite boiling.
33179485	17	52	theme	K	2277:2277	arg1	"					2281:2281	The crystal former "Ester K 01"	2251:2281	The crystal former "Ester K 01"	2251:2281	The crystal former "Ester K 01" was studied in production and laboratory conditions, and the crystallizing agents' comparative characteristics were also given.
33179485	18	53	theme	proces	2511:2516	arg1	conditions					2518:2527	the fully automated proces conditions	2491:2527	the fully automated proces conditions for producing massecuite	2491:2552	CONCLUSIONS In conclusion, the usage of the crystallizing agent "Ester K 01" in the fully automated proces conditions for producing massecuite would be recommended by us.
33179485	8	54	theme	qualitative	1015:1025	arg1	indicators					1044:1053	qualitative and quantitative indicators	1015:1053	qualitative and quantitative indicators of products and intermediates	1015:1083	All the studies of qualitative and quantitative indicators of products and intermediates were performed using standard methods.
33179485	6	55	from	evaporator	855:864	arg1	conditions					869:878	conditions	869:878	conditions maximally adapted to production	869:910	Prepared syrup samples were used to obtain the massecuite, which was boiled in a laboratory vacuum evaporator in conditions maximally adapted to production.
33179485	16	56	theme	sugar	2160:2164	arg1	crystals					2166:2173	sugar crystals	2160:2173	sugar crystals obtained with different crystal formers	2160:2213	The granulometric composition of sugar crystals obtained with different crystal formers was also compared in this research.
33179485	3	57	theme	01	447:448	arg1	"					449:449	the crystal former "Ester K 01"	419:449	the crystal former "Ester K 01"	419:449	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	8	58	theme	quantitative	1031:1042	arg1	indicators					1044:1053	qualitative and quantitative indicators	1015:1053	qualitative and quantitative indicators of products and intermediates	1015:1083	All the studies of qualitative and quantitative indicators of products and intermediates were performed using standard methods.
33179485	17	59	theme	crystallizing	2344:2356	arg1	agents					2358:2363	the crystallizing agents'	2340:2364	the crystallizing agents' comparative characteristics	2340:2392	The crystal former "Ester K 01" was studied in production and laboratory conditions, and the crystallizing agents' comparative characteristics were also given.
33179485	3	60	dep	types	342:346	arg1	"					449:449	the crystal former "Ester K 01"	419:449	the crystal former "Ester K 01"	419:449	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	60	dep	types	342:346	arg1	types					342:346	different types	332:346	different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product	332:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	60	dep	types	342:346	arg1	suspension					403:412	a standard factory suspension	384:412	a standard factory suspension	384:412	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	60	dep	types	342:346	arg1	sugar					377:381	powdered sugar	368:381	powdered sugar	368:381	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	60	dep	types	342:346	arg1	comparison					465:474	a comparison	463:474	a comparison of their direct effects on the quality of the finished product	463:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	10	61	theme	particle	1278:1285	arg1	distribution					1287:1298	The most uniform particle distribution	1261:1298	The most uniform particle distribution	1261:1298	The most uniform particle distribution was noted using "Ester K 01" crystal former.
33179485	3	62	theme	Ester	439:443	arg1	"					449:449	the crystal former "Ester K 01"	419:449	the crystal former "Ester K 01"	419:449	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	6	63	theme	syrup	765:769	arg1	samples					771:777	Prepared syrup samples	756:777	Prepared syrup samples	756:777	Prepared syrup samples were used to obtain the massecuite, which was boiled in a laboratory vacuum evaporator in conditions maximally adapted to production.
33179485	3	64	from	suspension	403:412	arg1	quality					507:513	the quality	503:513	the quality of the finished product	503:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	6	65	theme	laboratory	837:846	arg1	evaporator					855:864	a laboratory vacuum evaporator	835:864	a laboratory vacuum evaporator in conditions maximally adapted to production	835:910	Prepared syrup samples were used to obtain the massecuite, which was boiled in a laboratory vacuum evaporator in conditions maximally adapted to production.
33179485	15	66	theme	equal	1971:1975	arg1	crystals					1977:1984	The most equal crystals	1962:1984	The most equal crystals	1962:1984	The most equal crystals were produced using an "Ester K 01" crystallizing agent and a standard factory suspension in comparison to massecuite made with icing sugar.
33179485	18	67	theme	crystallizing	2455:2467	arg1	"					2486:2486	the crystallizing agent "Ester K 01"	2451:2486	the crystallizing agent "Ester K 01"	2451:2486	CONCLUSIONS In conclusion, the usage of the crystallizing agent "Ester K 01" in the fully automated proces conditions for producing massecuite would be recommended by us.
33179485	16	68	theme	granulometric	2131:2143	arg1	composition					2145:2155	The granulometric composition	2127:2155	The granulometric composition of sugar crystals obtained with different crystal formers	2127:2213	The granulometric composition of sugar crystals obtained with different crystal formers was also compared in this research.
33179485	18	69	theme	"	2486:2486	arg1	usage					2442:2446	the usage	2438:2446	the usage of the crystallizing agent "Ester K 01" in the fully automated proces conditions for producing massecuite	2438:2552	CONCLUSIONS In conclusion, the usage of the crystallizing agent "Ester K 01" in the fully automated proces conditions for producing massecuite would be recommended by us.
33179485	11	70	theme	massecuite	1496:1505	arg1	syrup					1507:1511	the obtained massecuite and massecuite syrup	1468:1511	the obtained massecuite and massecuite syrup in accordance with the type of crystal former used	1468:1562	The duration of massecuite boiling was studied and a comparative characteristic of the quality indicators was provided for the obtained massecuite and massecuite syrup in accordance with the type of crystal former used.
33179485	15	71	theme	standard	2048:2055	arg1	suspension					2065:2074	a standard factory suspension	2046:2074	a standard factory suspension in comparison	2046:2088	The most equal crystals were produced using an "Ester K 01" crystallizing agent and a standard factory suspension in comparison to massecuite made with icing sugar.
33179485	17	72	theme	crystal	2255:2261	arg1	"					2281:2281	The crystal former "Ester K 01"	2251:2281	The crystal former "Ester K 01"	2251:2281	The crystal former "Ester K 01" was studied in production and laboratory conditions, and the crystallizing agents' comparative characteristics were also given.
33179485	13	73	theme	ash	1711:1713	arg1	content					1715:1721	the lowest ash content	1700:1721	the lowest ash content	1700:1721	This sample also had the lowest ash content.
33179485	9	74	theme	size	1145:1148	arg1	distribution					1150:1161	The particle size distribution	1132:1161	The particle size distribution	1132:1161	RESULTS The particle size distribution was determined and the curves of the differential size distribution of sugar fineness were given.
33179485	2	75	theme	product	260:266	arg1	quality					236:242	the quality	232:242	the quality of the finished product	232:266	The intensification of this process is possible by reducing the boiling time and thus improving the quality of the finished product.
33179485	4	76	theme	agents	588:593	arg1	tests					563:567	All laboratory tests	548:567	METHODS All laboratory tests of crystal-forming agents	540:593	METHODS All laboratory tests of crystal-forming agents were carried out using syrup taken from sugar factories.
33179485	3	77	from	"	449:449	arg1	quality					507:513	the quality	503:513	the quality of the finished product	503:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	78	theme	direct	485:490	arg1	effects					492:498	their direct effects	479:498	their direct effects on the quality of the finished product	479:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	15	79	theme	01	2018:2019	arg1	"					2020:2020	an "Ester K 01"	2006:2020	an "Ester K 01" crystallizing agent and a standard factory suspension in comparison	2006:2088	The most equal crystals were produced using an "Ester K 01" crystallizing agent and a standard factory suspension in comparison to massecuite made with icing sugar.
33179485	16	80	theme	different	2189:2197	arg1	formers					2207:2213	different crystal formers	2189:2213	different crystal formers	2189:2213	The granulometric composition of sugar crystals obtained with different crystal formers was also compared in this research.
33179485	15	81	theme	factory	2057:2063	arg1	suspension					2065:2074	a standard factory suspension	2046:2074	a standard factory suspension in comparison	2046:2088	The most equal crystals were produced using an "Ester K 01" crystallizing agent and a standard factory suspension in comparison to massecuite made with icing sugar.
33179485	4	82	theme	laboratory	552:561	arg1	tests					563:567	All laboratory tests	548:567	METHODS All laboratory tests of crystal-forming agents	540:593	METHODS All laboratory tests of crystal-forming agents were carried out using syrup taken from sugar factories.
33179485	15	83	theme	Ester	2010:2014	arg1	"					2020:2020	an "Ester K 01"	2006:2020	an "Ester K 01" crystallizing agent and a standard factory suspension in comparison	2006:2088	The most equal crystals were produced using an "Ester K 01" crystallizing agent and a standard factory suspension in comparison to massecuite made with icing sugar.
33179485	15	84	theme	K	2016:2016	arg1	"					2020:2020	an "Ester K 01"	2006:2020	an "Ester K 01" crystallizing agent and a standard factory suspension in comparison	2006:2088	The most equal crystals were produced using an "Ester K 01" crystallizing agent and a standard factory suspension in comparison to massecuite made with icing sugar.
33179485	7	85	from	massecuite	950:959	arg1	filtration					984:993	a laboratory vacuum filtration	964:993	a laboratory vacuum filtration	964:993	Sugar was obtained by separating the massecuite in a laboratory vacuum filtration.
33179485	7	86	theme	laboratory	966:975	arg1	filtration					984:993	a laboratory vacuum filtration	964:993	a laboratory vacuum filtration	964:993	Sugar was obtained by separating the massecuite in a laboratory vacuum filtration.
33179485	4	87	theme	sugar	635:639	arg1	factories					641:649	sugar factories	635:649	sugar factories	635:649	METHODS All laboratory tests of crystal-forming agents were carried out using syrup taken from sugar factories.
33179485	11	88	theme	quality	1432:1438	arg1	indicators					1440:1449	the quality indicators	1428:1449	the quality indicators	1428:1449	The duration of massecuite boiling was studied and a comparative characteristic of the quality indicators was provided for the obtained massecuite and massecuite syrup in accordance with the type of crystal former used.
33179485	4	89	dep	METHODS	540:546	arg1	tests					563:567	All laboratory tests	548:567	METHODS All laboratory tests of crystal-forming agents	540:593	METHODS All laboratory tests of crystal-forming agents were carried out using syrup taken from sugar factories.
33179485	1	90	theme	massecuite	116:125	arg1	boiling					127:133	massecuite boiling	116:133	massecuite boiling	116:133	BACKGROUND One of the important processes in sugar production is massecuite boiling.
33179485	3	91	theme	product	531:537	arg1	quality					507:513	the quality	503:513	the quality of the finished product	503:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	10	92	theme	uniform	1270:1276	arg1	distribution					1287:1298	The most uniform particle distribution	1261:1298	The most uniform particle distribution	1261:1298	The most uniform particle distribution was noted using "Ester K 01" crystal former.
33179485	12	93	theme	K	1652:1652	arg1	"					1656:1656	the "Ester K 01"	1641:1656	the "Ester K 01" crystallizing agent	1641:1676	The highest sucrose content was observed in the sugar sample obtained using the "Ester K 01" crystallizing agent.
33179485	12	94	located	observed	1597:1604	arg1	sample					1619:1624	the sugar sample	1609:1624	the sugar sample obtained using the "Ester K 01" crystallizing agent	1609:1676	The highest sucrose content was observed in the sugar sample obtained using the "Ester K 01" crystallizing agent.
33179485	12	94	located	observed	1597:1604	arg2	content					1585:1591	The highest sucrose content	1565:1591	The highest sucrose content	1565:1591	The highest sucrose content was observed in the sugar sample obtained using the "Ester K 01" crystallizing agent.
33179485	16	95	theme	crystal	2199:2205	arg1	formers					2207:2213	different crystal formers	2189:2213	different crystal formers	2189:2213	The granulometric composition of sugar crystals obtained with different crystal formers was also compared in this research.
33179485	5	96	theme	particle	699:706	arg1	dispersion					714:723	dispersion	714:723	dispersion	714:723	Laser diffractometry was used to determine the particle size (dispersion) of the crystalforming agents.
33179485	5	96	theme	particle	699:706	arg1	size					708:711	the particle size	695:711	the particle size (dispersion) of the crystalforming agents	695:753	Laser diffractometry was used to determine the particle size (dispersion) of the crystalforming agents.
33179485	9	97	theme	fineness	1240:1247	arg1	distribution					1218:1229	the differential size distribution	1196:1229	the differential size distribution of sugar fineness	1196:1247	RESULTS The particle size distribution was determined and the curves of the differential size distribution of sugar fineness were given.
33179485	14	98	theme	crystals	1754:1761	arg1	Microphotographs					1724:1739	Microphotographs	1724:1739	Microphotographs of the sugar crystals	1724:1761	Microphotographs of the sugar crystals were provided with fixation of their size after 5, 10, and 20 minutes of adding various types of crystallizing agent, and the morphology of the sugar crystals in the finished massecuite was studied.
33179485	3	99	theme	massecuite	304:313	arg1	boiling					315:321	massecuite boiling	304:321	massecuite boiling	304:321	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	14	100	from	massecuite	1938:1947	arg1	morphology					1889:1898	the morphology	1885:1898	the morphology of the sugar crystals in the finished massecuite	1885:1947	Microphotographs of the sugar crystals were provided with fixation of their size after 5, 10, and 20 minutes of adding various types of crystallizing agent, and the morphology of the sugar crystals in the finished massecuite was studied.
33179485	14	101	theme	crystals	1913:1920	arg1	morphology					1889:1898	the morphology	1885:1898	the morphology of the sugar crystals in the finished massecuite	1885:1947	Microphotographs of the sugar crystals were provided with fixation of their size after 5, 10, and 20 minutes of adding various types of crystallizing agent, and the morphology of the sugar crystals in the finished massecuite was studied.
33179485	15	102	from	suspension	2065:2074	arg1	comparison					2079:2088	comparison	2079:2088	comparison	2079:2088	The most equal crystals were produced using an "Ester K 01" crystallizing agent and a standard factory suspension in comparison to massecuite made with icing sugar.
33179485	18	103	theme	automated	2501:2509	arg1	conditions					2518:2527	the fully automated proces conditions	2491:2527	the fully automated proces conditions for producing massecuite	2491:2552	CONCLUSIONS In conclusion, the usage of the crystallizing agent "Ester K 01" in the fully automated proces conditions for producing massecuite would be recommended by us.
33179485	18	104	dep	CONCLUSIONS	2411:2421	arg1	recommended					2563:2573	recommended	2563:2573	would be recommended by us	2554:2579	CONCLUSIONS In conclusion, the usage of the crystallizing agent "Ester K 01" in the fully automated proces conditions for producing massecuite would be recommended by us.
33179485	19	105	theme	sugar	2638:2642	arg1	composition					2623:2633	more uniform granulometric composition	2596:2633	more uniform granulometric composition of sugar	2596:2642	It will allow more uniform granulometric composition of sugar to be obtained.
33179485	3	106	theme	different	332:340	arg1	types					342:346	different types	332:346	different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product	332:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	106	theme	different	332:340	arg1	suspension					403:412	a standard factory suspension	384:412	a standard factory suspension	384:412	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	106	theme	different	332:340	arg1	sugar					377:381	powdered sugar	368:381	powdered sugar	368:381	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	106	theme	different	332:340	arg1	comparison					465:474	a comparison	463:474	a comparison of their direct effects on the quality of the finished product	463:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	11	107	theme	massecuite	1361:1370	arg1	boiling					1372:1378	massecuite boiling	1361:1378	massecuite boiling	1361:1378	The duration of massecuite boiling was studied and a comparative characteristic of the quality indicators was provided for the obtained massecuite and massecuite syrup in accordance with the type of crystal former used.
33179485	18	108	from	usage	2442:2446	arg1	conditions					2518:2527	the fully automated proces conditions	2491:2527	the fully automated proces conditions for producing massecuite	2491:2552	CONCLUSIONS In conclusion, the usage of the crystallizing agent "Ester K 01" in the fully automated proces conditions for producing massecuite would be recommended by us.
33179485	1	109	theme	important	73:81	arg1	processes					83:91	the important processes	69:91	the important processes	69:91	BACKGROUND One of the important processes in sugar production is massecuite boiling.
33179485	3	110	theme	powdered	368:375	arg1	types					342:346	different types	332:346	different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product	332:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	110	theme	powdered	368:375	arg1	sugar					377:381	powdered sugar	368:381	powdered sugar	368:381	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	17	111	theme	former	2263:2268	arg1	"					2281:2281	The crystal former "Ester K 01"	2251:2281	The crystal former "Ester K 01"	2251:2281	The crystal former "Ester K 01" was studied in production and laboratory conditions, and the crystallizing agents' comparative characteristics were also given.
33179485	11	112	theme	comparative	1398:1408	arg1	characteristic					1410:1423	a comparative characteristic	1396:1423	a comparative characteristic of the quality indicators	1396:1449	The duration of massecuite boiling was studied and a comparative characteristic of the quality indicators was provided for the obtained massecuite and massecuite syrup in accordance with the type of crystal former used.
33179485	0	113	theme	agent	27:31	arg1	Influence					0:8	Influence	0:8	Influence of crystallizing agent on sugar quality	0:48	Influence of crystallizing agent on sugar quality.
33179485	12	114	theme	highest	1569:1575	arg1	content					1585:1591	The highest sucrose content	1565:1591	The highest sucrose content	1565:1591	The highest sucrose content was observed in the sugar sample obtained using the "Ester K 01" crystallizing agent.
33179485	3	115	theme	formers	359:365	arg1	types					342:346	different types	332:346	different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product	332:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	115	theme	formers	359:365	arg1	suspension					403:412	a standard factory suspension	384:412	a standard factory suspension	384:412	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	115	theme	formers	359:365	arg1	sugar					377:381	powdered sugar	368:381	powdered sugar	368:381	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	115	theme	formers	359:365	arg1	comparison					465:474	a comparison	463:474	a comparison of their direct effects on the quality of the finished product	463:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	17	116	theme	Ester	2271:2275	arg1	"					2281:2281	The crystal former "Ester K 01"	2251:2281	The crystal former "Ester K 01"	2251:2281	The crystal former "Ester K 01" was studied in production and laboratory conditions, and the crystallizing agents' comparative characteristics were also given.
33179485	9	117	theme	sugar	1234:1238	arg1	fineness					1240:1247	sugar fineness	1234:1247	sugar fineness	1234:1247	RESULTS The particle size distribution was determined and the curves of the differential size distribution of sugar fineness were given.
33179485	6	118	used	used	784:787	arg2	samples					771:777	Prepared syrup samples	756:777	Prepared syrup samples	756:777	Prepared syrup samples were used to obtain the massecuite, which was boiled in a laboratory vacuum evaporator in conditions maximally adapted to production.
33179485	19	119	theme	granulometric	2609:2621	arg1	composition					2623:2633	more uniform granulometric composition	2596:2633	more uniform granulometric composition of sugar	2596:2642	It will allow more uniform granulometric composition of sugar to be obtained.
33179485	3	120	theme	standard	386:393	arg1	types					342:346	different types	332:346	different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product	332:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	120	theme	standard	386:393	arg1	suspension					403:412	a standard factory suspension	384:412	a standard factory suspension	384:412	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	17	121	theme	01	2279:2280	arg1	"					2281:2281	The crystal former "Ester K 01"	2251:2281	The crystal former "Ester K 01"	2251:2281	The crystal former "Ester K 01" was studied in production and laboratory conditions, and the crystallizing agents' comparative characteristics were also given.
33179485	2	122	theme	process	164:170	arg1	possible					175:182	possible	175:182	possible	175:182	The intensification of this process is possible by reducing the boiling time and thus improving the quality of the finished product.
33179485	2	122	theme	process	164:170	arg1	intensification					140:154	The intensification	136:154	The intensification of this process	136:170	The intensification of this process is possible by reducing the boiling time and thus improving the quality of the finished product.
33179485	3	123	theme	crystal	423:429	arg1	"					449:449	the crystal former "Ester K 01"	419:449	the crystal former "Ester K 01"	419:449	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	8	124	theme	indicators	1044:1053	arg1	studies					1004:1010	All the studies	996:1010	All the studies of qualitative and quantitative indicators of products and intermediates	996:1083	All the studies of qualitative and quantitative indicators of products and intermediates were performed using standard methods.
33179485	14	125	theme	finished	1929:1936	arg1	massecuite					1938:1947	the finished massecuite	1925:1947	the finished massecuite	1925:1947	Microphotographs of the sugar crystals were provided with fixation of their size after 5, 10, and 20 minutes of adding various types of crystallizing agent, and the morphology of the sugar crystals in the finished massecuite was studied.
33179485	2	126	theme	boiling	200:206	arg1	time					208:211	the boiling time	196:211	the boiling time	196:211	The intensification of this process is possible by reducing the boiling time and thus improving the quality of the finished product.
33179485	15	127	theme	icing	2114:2118	arg1	sugar					2120:2124	icing sugar	2114:2124	icing sugar	2114:2124	The most equal crystals were produced using an "Ester K 01" crystallizing agent and a standard factory suspension in comparison to massecuite made with icing sugar.
33179485	8	128	theme	products	1058:1065	arg1	indicators					1044:1053	qualitative and quantitative indicators	1015:1053	qualitative and quantitative indicators of products and intermediates	1015:1083	All the studies of qualitative and quantitative indicators of products and intermediates were performed using standard methods.
33179485	14	129	theme	various	1843:1849	arg1	types					1851:1855	various types	1843:1855	various types of crystallizing agent	1843:1878	Microphotographs of the sugar crystals were provided with fixation of their size after 5, 10, and 20 minutes of adding various types of crystallizing agent, and the morphology of the sugar crystals in the finished massecuite was studied.
33179485	3	130	theme	finished	522:529	arg1	product					531:537	the finished product	518:537	the finished product	518:537	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	3	131	theme	K	445:445	arg1	"					449:449	the crystal former "Ester K 01"	419:449	the crystal former "Ester K 01"	419:449	This study examines the methods of massecuite boiling by using different types of crystal formers: powdered sugar, a standard factory suspension, and the crystal former "Ester K 01", as well as a comparison of their direct effects on the quality of the finished product.
33179485	8	132	theme	intermediates	1071:1083	arg1	indicators					1044:1053	qualitative and quantitative indicators	1015:1053	qualitative and quantitative indicators of products and intermediates	1015:1083	All the studies of qualitative and quantitative indicators of products and intermediates were performed using standard methods.
33179485	6	133	theme	Prepared	756:763	arg1	samples					771:777	Prepared syrup samples	756:777	Prepared syrup samples	756:777	Prepared syrup samples were used to obtain the massecuite, which was boiled in a laboratory vacuum evaporator in conditions maximally adapted to production.
33179485	15	134	theme	most	1966:1969	arg1	crystals					1977:1984	The most equal crystals	1962:1984	The most equal crystals	1962:1984	The most equal crystals were produced using an "Ester K 01" crystallizing agent and a standard factory suspension in comparison to massecuite made with icing sugar.
33179485	17	135	theme	comparative	2366:2376	arg1	characteristics					2378:2392	the crystallizing agents' comparative characteristics	2340:2392	the crystallizing agents' comparative characteristics	2340:2392	The crystal former "Ester K 01" was studied in production and laboratory conditions, and the crystallizing agents' comparative characteristics were also given.
33179485	18	136	theme	agent	2469:2473	arg1	"					2486:2486	the crystallizing agent "Ester K 01"	2451:2486	the crystallizing agent "Ester K 01"	2451:2486	CONCLUSIONS In conclusion, the usage of the crystallizing agent "Ester K 01" in the fully automated proces conditions for producing massecuite would be recommended by us.
33179485	11	137	theme	former	1552:1557	arg1	type					1536:1539	the type	1532:1539	the type of crystal former used	1532:1562	The duration of massecuite boiling was studied and a comparative characteristic of the quality indicators was provided for the obtained massecuite and massecuite syrup in accordance with the type of crystal former used.
33263583	7	0	theme	suction	1152:1158	arg1	time					1160:1163	capillary suction time	1142:1163	capillary suction time (CST)	1142:1169	With the continuous addition of CDF and PAM, the corresponding index of capillary suction time (CST) increased.
33263583	7	0	theme	suction	1152:1158	arg1	CST					1166:1168	CST	1166:1168	CST	1166:1168	With the continuous addition of CDF and PAM, the corresponding index of capillary suction time (CST) increased.
33263583	5	1	dep	sludge	843:848	arg1	pH					864:865	pH	864:865	pH	864:865	The fermentation process was also analyzed by testing the performance of dewatered sludge (temperature, pH, ammonia nitrogen, E4/E6 (humic acid absorbance at 465 nm (E4) and 665 nm (E6))).
33263583	5	1	dep	sludge	843:848	arg1	nitrogen					876:883	ammonia nitrogen	868:883	ammonia nitrogen	868:883	The fermentation process was also analyzed by testing the performance of dewatered sludge (temperature, pH, ammonia nitrogen, E4/E6 (humic acid absorbance at 465 nm (E4) and 665 nm (E6))).
33263583	5	1	dep	sludge	843:848	arg1	temperature					851:861	temperature	851:861	temperature	851:861	The fermentation process was also analyzed by testing the performance of dewatered sludge (temperature, pH, ammonia nitrogen, E4/E6 (humic acid absorbance at 465 nm (E4) and 665 nm (E6))).
33263583	5	1	dep	sludge	843:848	arg1	E4/E6					886:890	E4/E6	886:890	E4/E6 (humic acid absorbance at 465 nm (E4) and 665 nm (E6))	886:945	The fermentation process was also analyzed by testing the performance of dewatered sludge (temperature, pH, ammonia nitrogen, E4/E6 (humic acid absorbance at 465 nm (E4) and 665 nm (E6))).
33263583	9	2	with	index	1288:1292	arg1	CDF					1336:1338	CDF	1336:1338	CDF	1336:1338	In addition, the sludge index of pumping time and moisture content with CDF were low in contrast with PAM.
33263583	4	3	theme	effluent	712:719	arg1	nitrogen					729:736	effluent ammonia nitrogen	712:736	effluent ammonia nitrogen	712:736	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	8	4	with	value	1202:1206	arg1	CDF					1220:1222	CDF	1220:1222	CDF	1220:1222	Moreover, the total value of CST with CDF was low, showing a good dewaterability.
33263583	4	5	theme	sludge	608:613	arg1	dewatering					615:624	sludge dewatering	608:624	sludge dewatering	608:624	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	0	6	theme	recycling	87:95	arg1	properties					97:106	dewatering and recycling properties	72:106	dewatering and recycling properties	72:106	Application of composite degradable modified starch-based flocculant on dewatering and recycling properties.
33263583	5	7	from	nm	938:939	arg1	absorbance					904:913	humic acid absorbance	893:913	humic acid absorbance at 465 nm (E4) and 665 nm (E6)	893:944	The fermentation process was also analyzed by testing the performance of dewatered sludge (temperature, pH, ammonia nitrogen, E4/E6 (humic acid absorbance at 465 nm (E4) and 665 nm (E6))).
33263583	6	8	theme	easy	1037:1040	arg1	transportation					1042:1055	easy transportation	1037:1055	easy transportation	1037:1055	The dehydrated sludge with CDF could be easily compressed into cakes by belt-filter for easy transportation and storage.
33263583	2	9	theme	novel	295:299	arg1	initiator					301:309	a novel initiator	293:309	a novel initiator	293:309	Composite degradable flocculant (CDF) was prepared by cationic polyacrylamide (PAM) grafting onto modified starch with a novel initiator, and characterized by Fourier transform infrared spectroscopy.
33263583	9	10	theme	moisture	1314:1321	arg1	content					1323:1329	moisture content	1314:1329	moisture content	1314:1329	In addition, the sludge index of pumping time and moisture content with CDF were low in contrast with PAM.
33263583	7	11	theme	capillary	1142:1150	arg1	time					1160:1163	capillary suction time	1142:1163	capillary suction time (CST)	1142:1169	With the continuous addition of CDF and PAM, the corresponding index of capillary suction time (CST) increased.
33263583	7	11	theme	capillary	1142:1150	arg1	CST					1166:1168	CST	1166:1168	CST	1166:1168	With the continuous addition of CDF and PAM, the corresponding index of capillary suction time (CST) increased.
33263583	2	12	theme	Composite	174:182	arg1	CDF					207:209	CDF	207:209	CDF	207:209	Composite degradable flocculant (CDF) was prepared by cationic polyacrylamide (PAM) grafting onto modified starch with a novel initiator, and characterized by Fourier transform infrared spectroscopy.
33263583	2	12	theme	Composite	174:182	arg1	flocculant					195:204	Composite degradable flocculant	174:204	Composite degradable flocculant (CDF)	174:210	Composite degradable flocculant (CDF) was prepared by cationic polyacrylamide (PAM) grafting onto modified starch with a novel initiator, and characterized by Fourier transform infrared spectroscopy.
33263583	9	13	from	addition	1267:1274	arg1	low					1345:1347	low	1345:1347	low	1345:1347	In addition, the sludge index of pumping time and moisture content with CDF were low in contrast with PAM.
33263583	9	13	from	addition	1267:1274	arg1	index					1288:1292	the sludge index	1277:1292	the sludge index of pumping time and moisture content with CDF	1277:1338	In addition, the sludge index of pumping time and moisture content with CDF were low in contrast with PAM.
33263583	5	14	theme	acid	899:902	arg1	absorbance					904:913	humic acid absorbance	893:913	humic acid absorbance at 465 nm (E4) and 665 nm (E6)	893:944	The fermentation process was also analyzed by testing the performance of dewatered sludge (temperature, pH, ammonia nitrogen, E4/E6 (humic acid absorbance at 465 nm (E4) and 665 nm (E6))).
33263583	9	15	theme	content	1323:1329	arg1	low					1345:1347	low	1345:1347	low	1345:1347	In addition, the sludge index of pumping time and moisture content with CDF were low in contrast with PAM.
33263583	9	15	theme	content	1323:1329	arg1	index					1288:1292	the sludge index	1277:1292	the sludge index of pumping time and moisture content with CDF	1277:1338	In addition, the sludge index of pumping time and moisture content with CDF were low in contrast with PAM.
33263583	7	16	theme	corresponding	1119:1131	arg1	index					1133:1137	the corresponding index	1115:1137	the corresponding index of capillary suction time (CST)	1115:1169	With the continuous addition of CDF and PAM, the corresponding index of capillary suction time (CST) increased.
33263583	0	17	from	flocculant	58:67	arg1	properties					97:106	dewatering and recycling properties	72:106	dewatering and recycling properties	72:106	Application of composite degradable modified starch-based flocculant on dewatering and recycling properties.
33263583	7	18	theme	PAM	1110:1112	arg1	addition					1090:1097	the continuous addition	1075:1097	the continuous addition of CDF and PAM	1075:1112	With the continuous addition of CDF and PAM, the corresponding index of capillary suction time (CST) increased.
33263583	4	19	from	flocculation	592:603	arg1	dewatering					615:624	sludge dewatering	608:624	sludge dewatering	608:624	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	4	19	from	flocculation	592:603	arg1	value					703:707	a lower value	695:707	a lower value of effluent ammonia nitrogen and total phosphorus	695:757	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	4	19	from	flocculation	592:603	arg1	rate					685:688	a higher transmittance and chemical oxygen demand removal rate	627:688	a higher transmittance and chemical oxygen demand removal rate	627:688	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	5	20	theme	ammonia	868:874	arg1	nitrogen					876:883	ammonia nitrogen	868:883	ammonia nitrogen	868:883	The fermentation process was also analyzed by testing the performance of dewatered sludge (temperature, pH, ammonia nitrogen, E4/E6 (humic acid absorbance at 465 nm (E4) and 665 nm (E6))).
33263583	5	20	theme	ammonia	868:874	arg1	temperature					851:861	temperature	851:861	temperature	851:861	The fermentation process was also analyzed by testing the performance of dewatered sludge (temperature, pH, ammonia nitrogen, E4/E6 (humic acid absorbance at 465 nm (E4) and 665 nm (E6))).
33263583	5	21	theme	fermentation	764:775	arg1	process					777:783	The fermentation process	760:783	The fermentation process	760:783	The fermentation process was also analyzed by testing the performance of dewatered sludge (temperature, pH, ammonia nitrogen, E4/E6 (humic acid absorbance at 465 nm (E4) and 665 nm (E6))).
33263583	0	22	theme	starch-based	45:56	arg1	flocculant					58:67	starch-based flocculant	45:67	starch-based flocculant on dewatering and recycling properties	45:106	Application of composite degradable modified starch-based flocculant on dewatering and recycling properties.
33263583	5	23	from	nm	922:923	arg1	absorbance					904:913	humic acid absorbance	893:913	humic acid absorbance at 465 nm (E4) and 665 nm (E6)	893:944	The fermentation process was also analyzed by testing the performance of dewatered sludge (temperature, pH, ammonia nitrogen, E4/E6 (humic acid absorbance at 465 nm (E4) and 665 nm (E6))).
33263583	10	24	theme	low	1471:1473	arg1	value					1475:1479	low value	1471:1479	low value of E4/E6	1471:1488	Fermentation experiments demonstrated that sludge with CDF had a comparatively high temperature and low value of E4/E6.
33263583	10	25	theme	Fermentation	1371:1382	arg1	experiments					1384:1394	Fermentation experiments	1371:1394	Fermentation experiments	1371:1394	Fermentation experiments demonstrated that sludge with CDF had a comparatively high temperature and low value of E4/E6.
33263583	3	26	theme	sludge	408:413	arg1	microstructure					378:391	The microstructure	374:391	The microstructure of flocculated sludge	374:413	The microstructure of flocculated sludge was characterized by scanning electron microscopy.
33263583	4	27	theme	effective	582:590	arg1	flocculation					592:603	a highly effective flocculation	573:603	a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus	573:757	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	7	28	theme	time	1160:1163	arg1	index					1133:1137	the corresponding index	1115:1137	the corresponding index of capillary suction time (CST)	1115:1169	With the continuous addition of CDF and PAM, the corresponding index of capillary suction time (CST) increased.
33263583	2	29	dep	Fourier	333:339	arg1	transform					341:349	transform	341:349	transform infrared spectroscopy	341:371	Composite degradable flocculant (CDF) was prepared by cationic polyacrylamide (PAM) grafting onto modified starch with a novel initiator, and characterized by Fourier transform infrared spectroscopy.
33263583	4	30	theme	CDF	507:509	arg1	properties					493:502	the properties	489:502	the properties of CDF	489:509	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	5	31	theme	dewatered	833:841	arg1	sludge					843:848	dewatered sludge	833:848	dewatered sludge (temperature, pH, ammonia nitrogen, E4/E6 (humic acid absorbance at 465 nm (E4) and 665 nm (E6)))	833:946	The fermentation process was also analyzed by testing the performance of dewatered sludge (temperature, pH, ammonia nitrogen, E4/E6 (humic acid absorbance at 465 nm (E4) and 665 nm (E6))).
33263583	1	32	theme	Sludge	109:114	arg1	step					143:146	an important step	130:146	an important step for wastewater treatment	130:171	Sludge dewatering is an important step for wastewater treatment.
33263583	1	32	theme	Sludge	109:114	arg1	dewatering					116:125	Sludge dewatering	109:125	Sludge dewatering	109:125	Sludge dewatering is an important step for wastewater treatment.
33263583	9	33	theme	pumping	1297:1303	arg1	time					1305:1308	pumping time	1297:1308	pumping time	1297:1308	In addition, the sludge index of pumping time and moisture content with CDF were low in contrast with PAM.
33263583	0	34	theme	degradable	25:34	arg1	Application					0:10	Application	0:10	Application of composite degradable	0:34	Application of composite degradable modified starch-based flocculant on dewatering and recycling properties.
33263583	4	35	theme	phosphorus	748:757	arg1	dewatering					615:624	sludge dewatering	608:624	sludge dewatering	608:624	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	4	35	theme	phosphorus	748:757	arg1	value					703:707	a lower value	695:707	a lower value of effluent ammonia nitrogen and total phosphorus	695:757	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	4	35	theme	phosphorus	748:757	arg1	rate					685:688	a higher transmittance and chemical oxygen demand removal rate	627:688	a higher transmittance and chemical oxygen demand removal rate	627:688	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	8	36	theme	CST	1211:1213	arg1	low					1228:1230	low	1228:1230	low	1228:1230	Moreover, the total value of CST with CDF was low, showing a good dewaterability.
33263583	8	36	theme	CST	1211:1213	arg1	value					1202:1206	the total value	1192:1206	the total value of CST with CDF	1192:1222	Moreover, the total value of CST with CDF was low, showing a good dewaterability.
33263583	10	37	theme	E4/E6	1484:1488	arg1	temperature					1455:1465	a comparatively high temperature	1434:1465	a comparatively high temperature	1434:1465	Fermentation experiments demonstrated that sludge with CDF had a comparatively high temperature and low value of E4/E6.
33263583	10	37	theme	E4/E6	1484:1488	arg1	value					1475:1479	low value	1471:1479	low value of E4/E6	1471:1488	Fermentation experiments demonstrated that sludge with CDF had a comparatively high temperature and low value of E4/E6.
33263583	6	38	with	sludge	964:969	arg1	CDF					976:978	CDF	976:978	CDF	976:978	The dehydrated sludge with CDF could be easily compressed into cakes by belt-filter for easy transportation and storage.
33263583	4	39	theme	oxygen	663:668	arg1	demand					670:675	chemical oxygen demand	654:675	chemical oxygen demand	654:675	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	3	40	theme	flocculated	396:406	arg1	sludge					408:413	flocculated sludge	396:413	flocculated sludge	396:413	The microstructure of flocculated sludge was characterized by scanning electron microscopy.
33263583	2	41	dep	transform	341:349	arg1	infrared					351:358	infrared	351:358	transform infrared spectroscopy	341:371	Composite degradable flocculant (CDF) was prepared by cationic polyacrylamide (PAM) grafting onto modified starch with a novel initiator, and characterized by Fourier transform infrared spectroscopy.
33263583	4	42	theme	chemical	654:661	arg1	demand					670:675	chemical oxygen demand	654:675	chemical oxygen demand	654:675	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	3	43	theme	scanning	436:443	arg1	microscopy					454:463	scanning electron microscopy	436:463	scanning electron microscopy	436:463	The microstructure of flocculated sludge was characterized by scanning electron microscopy.
33263583	4	44	theme	ammonia	721:727	arg1	nitrogen					729:736	effluent ammonia nitrogen	712:736	effluent ammonia nitrogen	712:736	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	10	45	contain	had	1430:1432	arg2	value					1475:1479	low value	1471:1479	low value of E4/E6	1471:1488	Fermentation experiments demonstrated that sludge with CDF had a comparatively high temperature and low value of E4/E6.
33263583	10	45	contain	had	1430:1432	arg1	sludge					1414:1419	sludge	1414:1419	sludge with CDF	1414:1428	Fermentation experiments demonstrated that sludge with CDF had a comparatively high temperature and low value of E4/E6.
33263583	10	45	contain	had	1430:1432	arg2	temperature					1455:1465	a comparatively high temperature	1434:1465	a comparatively high temperature	1434:1465	Fermentation experiments demonstrated that sludge with CDF had a comparatively high temperature and low value of E4/E6.
33263583	2	46	theme	PAM	253:255	arg1	grafting					258:265	cationic polyacrylamide (PAM) grafting	228:265	cationic polyacrylamide (PAM) grafting onto modified starch with a novel initiator	228:309	Composite degradable flocculant (CDF) was prepared by cationic polyacrylamide (PAM) grafting onto modified starch with a novel initiator, and characterized by Fourier transform infrared spectroscopy.
33263583	4	47	theme	removal	677:683	arg1	rate					685:688	a higher transmittance and chemical oxygen demand removal rate	627:688	a higher transmittance and chemical oxygen demand removal rate	627:688	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	3	48	theme	electron	445:452	arg1	microscopy					454:463	scanning electron microscopy	436:463	scanning electron microscopy	436:463	The microstructure of flocculated sludge was characterized by scanning electron microscopy.
33263583	4	49	theme	prepared	550:557	arg1	CDF					559:561	the prepared CDF	546:561	the prepared CDF	546:561	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	7	50	theme	CDF	1102:1104	arg1	addition					1090:1097	the continuous addition	1075:1097	the continuous addition of CDF and PAM	1075:1112	With the continuous addition of CDF and PAM, the corresponding index of capillary suction time (CST) increased.
33263583	5	51	theme	sludge	843:848	arg1	performance					818:828	the performance	814:828	the performance of dewatered sludge (temperature, pH, ammonia nitrogen, E4/E6 (humic acid absorbance at 465 nm (E4) and 665 nm (E6)))	814:946	The fermentation process was also analyzed by testing the performance of dewatered sludge (temperature, pH, ammonia nitrogen, E4/E6 (humic acid absorbance at 465 nm (E4) and 665 nm (E6))).
33263583	9	52	theme	time	1305:1308	arg1	low					1345:1347	low	1345:1347	low	1345:1347	In addition, the sludge index of pumping time and moisture content with CDF were low in contrast with PAM.
33263583	9	52	theme	time	1305:1308	arg1	index					1288:1292	the sludge index	1277:1292	the sludge index of pumping time and moisture content with CDF	1277:1338	In addition, the sludge index of pumping time and moisture content with CDF were low in contrast with PAM.
33263583	4	53	theme	demand	670:675	arg1	rate					685:688	a higher transmittance and chemical oxygen demand removal rate	627:688	a higher transmittance and chemical oxygen demand removal rate	627:688	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	7	54	theme	continuous	1079:1088	arg1	addition					1090:1097	the continuous addition	1075:1097	the continuous addition of CDF and PAM	1075:1112	With the continuous addition of CDF and PAM, the corresponding index of capillary suction time (CST) increased.
33263583	2	55	theme	degradable	184:193	arg1	CDF					207:209	CDF	207:209	CDF	207:209	Composite degradable flocculant (CDF) was prepared by cationic polyacrylamide (PAM) grafting onto modified starch with a novel initiator, and characterized by Fourier transform infrared spectroscopy.
33263583	2	55	theme	degradable	184:193	arg1	flocculant					195:204	Composite degradable flocculant	174:204	Composite degradable flocculant (CDF)	174:210	Composite degradable flocculant (CDF) was prepared by cationic polyacrylamide (PAM) grafting onto modified starch with a novel initiator, and characterized by Fourier transform infrared spectroscopy.
33263583	2	56	theme	polyacrylamide	237:250	arg1	grafting					258:265	cationic polyacrylamide (PAM) grafting	228:265	cationic polyacrylamide (PAM) grafting onto modified starch with a novel initiator	228:309	Composite degradable flocculant (CDF) was prepared by cationic polyacrylamide (PAM) grafting onto modified starch with a novel initiator, and characterized by Fourier transform infrared spectroscopy.
33263583	11	57	theme	Such	1491:1494	arg1	CDF					1502:1504	Such novel CDF	1491:1504	Such novel CDF	1491:1504	Such novel CDF shows enormous potential in wastewater treatment and sludge fermentation.
33263583	4	58	theme	nitrogen	729:736	arg1	dewatering					615:624	sludge dewatering	608:624	sludge dewatering	608:624	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	4	58	theme	nitrogen	729:736	arg1	value					703:707	a lower value	695:707	a lower value of effluent ammonia nitrogen and total phosphorus	695:757	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	4	58	theme	nitrogen	729:736	arg1	rate					685:688	a higher transmittance and chemical oxygen demand removal rate	627:688	a higher transmittance and chemical oxygen demand removal rate	627:688	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	9	59	with	contrast	1352:1359	arg1	PAM					1366:1368	PAM	1366:1368	PAM	1366:1368	In addition, the sludge index of pumping time and moisture content with CDF were low in contrast with PAM.
33263583	2	60	theme	cationic	228:235	arg1	grafting					258:265	cationic polyacrylamide (PAM) grafting	228:265	cationic polyacrylamide (PAM) grafting onto modified starch with a novel initiator	228:309	Composite degradable flocculant (CDF) was prepared by cationic polyacrylamide (PAM) grafting onto modified starch with a novel initiator, and characterized by Fourier transform infrared spectroscopy.
33263583	1	61	theme	important	133:141	arg1	step					143:146	an important step	130:146	an important step for wastewater treatment	130:171	Sludge dewatering is an important step for wastewater treatment.
33263583	1	61	theme	important	133:141	arg1	dewatering					116:125	Sludge dewatering	109:125	Sludge dewatering	109:125	Sludge dewatering is an important step for wastewater treatment.
33263583	11	62	theme	enormous	1512:1519	arg1	potential					1521:1529	enormous potential	1512:1529	enormous potential	1512:1529	Such novel CDF shows enormous potential in wastewater treatment and sludge fermentation.
33263583	2	63	theme	modified	272:279	arg1	starch					281:286	modified starch	272:286	modified starch with a novel initiator	272:309	Composite degradable flocculant (CDF) was prepared by cationic polyacrylamide (PAM) grafting onto modified starch with a novel initiator, and characterized by Fourier transform infrared spectroscopy.
33263583	5	64	dep	E4/E6	886:890	arg1	absorbance					904:913	humic acid absorbance	893:913	humic acid absorbance at 465 nm (E4) and 665 nm (E6)	893:944	The fermentation process was also analyzed by testing the performance of dewatered sludge (temperature, pH, ammonia nitrogen, E4/E6 (humic acid absorbance at 465 nm (E4) and 665 nm (E6))).
33263583	4	65	theme	total	742:746	arg1	phosphorus					748:757	total phosphorus	742:757	total phosphorus	742:757	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	9	66	theme	sludge	1281:1286	arg1	low					1345:1347	low	1345:1347	low	1345:1347	In addition, the sludge index of pumping time and moisture content with CDF were low in contrast with PAM.
33263583	9	66	theme	sludge	1281:1286	arg1	index					1288:1292	the sludge index	1277:1292	the sludge index of pumping time and moisture content with CDF	1277:1338	In addition, the sludge index of pumping time and moisture content with CDF were low in contrast with PAM.
33263583	4	67	theme	higher	629:634	arg1	transmittance					636:648	a higher transmittance	627:648	a higher transmittance	627:648	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	9	68	from	contrast	1352:1359	arg1	low					1345:1347	low	1345:1347	low	1345:1347	In addition, the sludge index of pumping time and moisture content with CDF were low in contrast with PAM.
33263583	9	68	from	contrast	1352:1359	arg1	index					1288:1292	the sludge index	1277:1292	the sludge index of pumping time and moisture content with CDF	1277:1338	In addition, the sludge index of pumping time and moisture content with CDF were low in contrast with PAM.
33263583	8	69	theme	good	1243:1246	arg1	dewaterability					1248:1261	a good dewaterability	1241:1261	a good dewaterability	1241:1261	Moreover, the total value of CST with CDF was low, showing a good dewaterability.
33263583	5	70	theme	humic	893:897	arg1	absorbance					904:913	humic acid absorbance	893:913	humic acid absorbance at 465 nm (E4) and 665 nm (E6)	893:944	The fermentation process was also analyzed by testing the performance of dewatered sludge (temperature, pH, ammonia nitrogen, E4/E6 (humic acid absorbance at 465 nm (E4) and 665 nm (E6))).
33263583	11	71	theme	sludge	1559:1564	arg1	fermentation					1566:1577	sludge fermentation	1559:1577	sludge fermentation	1559:1577	Such novel CDF shows enormous potential in wastewater treatment and sludge fermentation.
33263583	2	72	with	starch	281:286	arg1	initiator					301:309	a novel initiator	293:309	a novel initiator	293:309	Composite degradable flocculant (CDF) was prepared by cationic polyacrylamide (PAM) grafting onto modified starch with a novel initiator, and characterized by Fourier transform infrared spectroscopy.
33263583	10	73	theme	high	1450:1453	arg1	temperature					1455:1465	a comparatively high temperature	1434:1465	a comparatively high temperature	1434:1465	Fermentation experiments demonstrated that sludge with CDF had a comparatively high temperature and low value of E4/E6.
33263583	8	74	theme	total	1196:1200	arg1	low					1228:1230	low	1228:1230	low	1228:1230	Moreover, the total value of CST with CDF was low, showing a good dewaterability.
33263583	8	74	theme	total	1196:1200	arg1	value					1202:1206	the total value	1192:1206	the total value of CST with CDF	1192:1222	Moreover, the total value of CST with CDF was low, showing a good dewaterability.
33263583	9	75	from	low	1345:1347	arg1	addition					1267:1274	addition	1267:1274	addition	1267:1274	In addition, the sludge index of pumping time and moisture content with CDF were low in contrast with PAM.
33263583	9	75	from	low	1345:1347	arg1	contrast					1352:1359	contrast	1352:1359	contrast with PAM	1352:1368	In addition, the sludge index of pumping time and moisture content with CDF were low in contrast with PAM.
33263583	1	76	theme	wastewater	152:161	arg1	treatment					163:171	wastewater treatment	152:171	wastewater treatment	152:171	Sludge dewatering is an important step for wastewater treatment.
33263583	0	77	theme	dewatering	72:81	arg1	properties					97:106	dewatering and recycling properties	72:106	dewatering and recycling properties	72:106	Application of composite degradable modified starch-based flocculant on dewatering and recycling properties.
33263583	11	78	theme	wastewater	1534:1543	arg1	treatment					1545:1553	wastewater treatment	1534:1553	wastewater treatment	1534:1553	Such novel CDF shows enormous potential in wastewater treatment and sludge fermentation.
33263583	6	79	theme	dehydrated	953:962	arg1	sludge					964:969	The dehydrated sludge	949:969	The dehydrated sludge with CDF	949:978	The dehydrated sludge with CDF could be easily compressed into cakes by belt-filter for easy transportation and storage.
33263583	11	80	theme	novel	1496:1500	arg1	CDF					1502:1504	Such novel CDF	1491:1504	Such novel CDF	1491:1504	Such novel CDF shows enormous potential in wastewater treatment and sludge fermentation.
33263583	4	81	theme	lower	697:701	arg1	value					703:707	a lower value	695:707	a lower value of effluent ammonia nitrogen and total phosphorus	695:757	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
33263583	10	82	with	sludge	1414:1419	arg1	CDF					1426:1428	CDF	1426:1428	CDF	1426:1428	Fermentation experiments demonstrated that sludge with CDF had a comparatively high temperature and low value of E4/E6.
33263583	4	83	theme	transmittance	636:648	arg1	rate					685:688	a higher transmittance and chemical oxygen demand removal rate	627:688	a higher transmittance and chemical oxygen demand removal rate	627:688	The study investigated the properties of CDF compared to PAM, which showed that the prepared CDF exhibited a highly effective flocculation on sludge dewatering, a higher transmittance and chemical oxygen demand removal rate, and a lower value of effluent ammonia nitrogen and total phosphorus.
34781802	7	0	theme	total	1180:1184	arg1	flow					1192:1195	total tract flow	1180:1195	total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose	1180:1264	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	3	1	theme	nutrient	510:517	arg1	utilisation					519:529	apparent total tract nutrient utilisation	489:529	apparent total tract nutrient utilisation	489:529	Excreta moisture content, ileal and caecal microbiota and short-chain fatty acid (SCFA) composition and apparent total tract nutrient utilisation were measured.2.
34781802	4	2	from	treatments	680:689	arg1	arrangement					712:722	a 2 × 2 factorial arrangement	694:722	a 2 × 2 factorial arrangement	694:722	Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg).
34781802	2	3	from	influence	195:203	arg1	pH					333:334	pH	333:334	pH	333:334	The objective of this study was to examine the influence of dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation on productive performance, viscosity and pH along the gastrointestinal tract in laying hens.
34781802	2	3	from	influence	195:203	arg1	viscosity					319:327	viscosity	319:327	viscosity	319:327	The objective of this study was to examine the influence of dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation on productive performance, viscosity and pH along the gastrointestinal tract in laying hens.
34781802	2	3	from	influence	195:203	arg1	performance					306:316	productive performance	295:316	productive performance	295:316	The objective of this study was to examine the influence of dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation on productive performance, viscosity and pH along the gastrointestinal tract in laying hens.
34781802	13	4	theme	sNSP	2074:2077	arg1	level					2079:2083	dietary sNSP level	2066:2083	dietary sNSP level	2066:2083	These results reiterated the ability of xylanase to improve nutrient digestibility and reduce excreta moisture content in laying hens, and highlighted the importance of considering dietary sNSP level in laying hen diets.
34781802	2	5	theme	xylanase	267:274	arg1	supplementation					276:290	dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation	208:290	supplementation	276:290	The objective of this study was to examine the influence of dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation on productive performance, viscosity and pH along the gastrointestinal tract in laying hens.
34781802	2	6	from	tract	363:367	arg1	hens					379:382	laying hens	372:382	laying hens	372:382	The objective of this study was to examine the influence of dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation on productive performance, viscosity and pH along the gastrointestinal tract in laying hens.
34781802	4	7	theme	wheat-based	660:670	arg1	treatments					680:689	the four wheat-based dietary treatments	651:689	the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg)	651:839	Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg).
34781802	10	8	theme	anaerobic	1686:1694	arg1	P < 0.05					1706:1713	P < 0.05	1706:1713	P < 0.05	1706:1713	and total anaerobic bacteria (P < 0.05).
34781802	10	8	theme	anaerobic	1686:1694	arg1	bacteria					1696:1703	anaerobic bacteria	1686:1703	anaerobic bacteria (P < 0.05)	1686:1714	and total anaerobic bacteria (P < 0.05).
34781802	6	9	theme	jejunum	926:932	arg1	viscosity					934:942	jejunum viscosity	926:942	jejunum viscosity	926:942	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	9	10	theme	isobutyric	1606:1615	arg1	acid					1630:1633	and isobutyric and succinic acid	1602:1633	and isobutyric and succinic acid	1602:1633	and isobutyric and succinic acid, and ileal concentration of Bacillus sp.
34781802	8	11	with	Supplementation	1378:1392	arg1	xylanase					1399:1406	xylanase	1399:1406	xylanase	1399:1406	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	12	12	theme	valeric	1858:1864	arg1	acid					1866:1869	valeric acid	1858:1869	valeric acid (P < 0.05)	1858:1880	and valeric acid (P < 0.05).5.
34781802	12	12	theme	valeric	1858:1864	arg1	P < 0.05					1872:1879	P < 0.05	1872:1879	P < 0.05	1872:1879	and valeric acid (P < 0.05).5.
34781802	9	13	theme	succinic	1621:1628	arg1	acid					1630:1633	and isobutyric and succinic acid	1602:1633	and isobutyric and succinic acid	1602:1633	and isobutyric and succinic acid, and ileal concentration of Bacillus sp.
34781802	0	14	from	environment	92:102	arg1	hens					139:142	laying hens	132:142	laying hens	132:142	Dietary soluble non-starch polysaccharide level and xylanase influence the gastrointestinal environment and nutrient utilisation in laying hens.
34781802	13	15	from	content	1996:2002	arg1	hens					2014:2017	laying hens	2007:2017	laying hens	2007:2017	These results reiterated the ability of xylanase to improve nutrient digestibility and reduce excreta moisture content in laying hens, and highlighted the importance of considering dietary sNSP level in laying hen diets.
34781802	2	16	theme	polysaccharide	235:248	arg1	level					257:261	dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation	208:290	level	257:261	The objective of this study was to examine the influence of dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation on productive performance, viscosity and pH along the gastrointestinal tract in laying hens.
34781802	4	17	theme	factorial	702:710	arg1	arrangement					712:722	a 2 × 2 factorial arrangement	694:722	a 2 × 2 factorial arrangement	694:722	Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg).
34781802	11	18	theme	Xylanase	1717:1724	arg1	application					1726:1736	Xylanase application	1717:1736	Xylanase application	1717:1736	Xylanase application also increased energy retention and insoluble and total NSP degradation, and caecal abundance of Bifidobacteria sp.
34781802	2	19	theme	soluble	216:222	arg1	level					257:261	dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation	208:290	level	257:261	The objective of this study was to examine the influence of dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation on productive performance, viscosity and pH along the gastrointestinal tract in laying hens.
34781802	5	20	theme	dietary	861:867	arg1	treatments					869:878	the dietary treatments	857:878	the dietary treatments for 56 d.3	857:889	Birds were fed the dietary treatments for 56 d.3.
34781802	9	21	theme	ileal	1640:1644	arg1	concentration					1646:1658	ileal concentration	1640:1658	ileal concentration of Bacillus sp	1640:1673	and isobutyric and succinic acid, and ileal concentration of Bacillus sp.
34781802	7	22	theme	lactic	1288:1293	arg1	P < 0.05					1316:1323	P < 0.05	1316:1323	P < 0.05	1316:1323	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	7	22	theme	lactic	1288:1293	arg1	concentrations					1300:1313	ileal acetic and lactic acid concentrations	1271:1313	ileal acetic and lactic acid concentrations (P < 0.05)	1271:1324	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	2	23	theme	level	257:261	arg1	influence					195:203	the influence	191:203	the influence of dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation on productive performance, viscosity and pH	191:334	The objective of this study was to examine the influence of dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation on productive performance, viscosity and pH along the gastrointestinal tract in laying hens.
34781802	6	24	theme	total	973:977	arg1	flow					985:988	total tract flow	973:988	total tract flow of insoluble arabinose	973:1011	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	7	25	theme	acetic	1277:1282	arg1	P < 0.05					1316:1323	P < 0.05	1316:1323	P < 0.05	1316:1323	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	7	25	theme	acetic	1277:1282	arg1	concentrations					1300:1313	ileal acetic and lactic acid concentrations	1271:1313	ileal acetic and lactic acid concentrations (P < 0.05)	1271:1324	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	6	26	theme	arabinose	1003:1011	arg1	flow					985:988	total tract flow	973:988	total tract flow of insoluble arabinose	973:1011	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	6	26	theme	arabinose	1003:1011	arg1	concentration					1032:1044	succinic acid concentration	1018:1044	succinic acid concentration in the caeca (P < 0.05)	1018:1068	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	6	26	theme	arabinose	1003:1011	arg1	degradability					945:957	degradability	945:957	degradability of total NSP	945:970	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	6	26	theme	arabinose	1003:1011	arg1	viscosity					934:942	jejunum viscosity	926:942	jejunum viscosity	926:942	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	4	27	theme	treatments	680:689	arg1	one					644:646	one	644:646	one	644:646	Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg).
34781802	4	27	theme	treatments	680:689	arg1	treatments					680:689	the four wheat-based dietary treatments	651:689	the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg)	651:839	Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg).
34781802	11	28	theme	caecal	1815:1820	arg1	abundance					1822:1830	caecal abundance	1815:1830	caecal abundance of Bifidobacteria sp	1815:1851	Xylanase application also increased energy retention and insoluble and total NSP degradation, and caecal abundance of Bifidobacteria sp.
34781802	0	29	theme	non-starch	16:25	arg1	level					42:46	Dietary soluble non-starch polysaccharide level	0:46	Dietary soluble non-starch polysaccharide level	0:46	Dietary soluble non-starch polysaccharide level and xylanase influence the gastrointestinal environment and nutrient utilisation in laying hens.
34781802	6	30	theme	NSP	968:970	arg1	flow					985:988	total tract flow	973:988	total tract flow of insoluble arabinose	973:1011	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	6	30	theme	NSP	968:970	arg1	concentration					1032:1044	succinic acid concentration	1018:1044	succinic acid concentration in the caeca (P < 0.05)	1018:1068	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	6	30	theme	NSP	968:970	arg1	degradability					945:957	degradability	945:957	degradability of total NSP	945:970	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	6	30	theme	NSP	968:970	arg1	viscosity					934:942	jejunum viscosity	926:942	jejunum viscosity	926:942	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	9	31	theme	sp	1672:1673	arg1	acid					1630:1633	and isobutyric and succinic acid	1602:1633	and isobutyric and succinic acid	1602:1633	and isobutyric and succinic acid, and ileal concentration of Bacillus sp.
34781802	9	31	theme	sp	1672:1673	arg1	concentration					1646:1658	ileal concentration	1640:1658	ileal concentration of Bacillus sp	1640:1673	and isobutyric and succinic acid, and ileal concentration of Bacillus sp.
34781802	7	32	theme	free	1158:1161	arg1	oligosaccharide					1163:1177	free oligosaccharide	1158:1177	free oligosaccharide	1158:1177	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	13	33	theme	moisture	1987:1994	arg1	content					1996:2002	excreta moisture content	1979:2002	excreta moisture content in laying hens	1979:2017	These results reiterated the ability of xylanase to improve nutrient digestibility and reduce excreta moisture content in laying hens, and highlighted the importance of considering dietary sNSP level in laying hen diets.
34781802	8	34	theme	jejunum	1428:1434	arg1	viscosity					1446:1454	reduced jejunum and ileum viscosity	1420:1454	reduced jejunum and ileum viscosity	1420:1454	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	8	34	theme	jejunum	1428:1434	arg1	pH					1464:1465	caecal pH	1457:1465	caecal pH	1457:1465	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	4	35	theme	sNSP	753:756	arg1	levels					743:748	two levels	739:748	two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg)	739:792	Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg).
34781802	0	36	theme	nutrient	108:115	arg1	utilisation					117:127	nutrient utilisation	108:127	nutrient utilisation	108:127	Dietary soluble non-starch polysaccharide level and xylanase influence the gastrointestinal environment and nutrient utilisation in laying hens.
34781802	8	37	theme	ileum	1440:1444	arg1	viscosity					1446:1454	reduced jejunum and ileum viscosity	1420:1454	reduced jejunum and ileum viscosity	1420:1454	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	8	37	theme	ileum	1440:1444	arg1	pH					1464:1465	caecal pH	1457:1465	caecal pH	1457:1465	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	6	38	from	degradability	945:957	arg1	P < 0.05					1060:1067	P < 0.05	1060:1067	P < 0.05	1060:1067	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	6	38	from	degradability	945:957	arg1	caeca					1053:1057	the caeca	1049:1057	the caeca (P < 0.05)	1049:1068	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	4	39	theme	High	759:762	arg1	13.40 g/kg					764:773	High 13.40 g/kg	759:773	High 13.40 g/kg	759:773	Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg).
34781802	3	40	theme	short-chain	443:453	arg1	composition					473:483	ileal and caecal microbiota and short-chain fatty acid (SCFA) composition	411:483	composition	473:483	Excreta moisture content, ileal and caecal microbiota and short-chain fatty acid (SCFA) composition and apparent total tract nutrient utilisation were measured.2.
34781802	6	41	theme	succinic	1018:1025	arg1	concentration					1032:1044	succinic acid concentration	1018:1044	succinic acid concentration in the caeca (P < 0.05)	1018:1068	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	3	42	theme	moisture	393:400	arg1	content					402:408	Excreta moisture content	385:408	Excreta moisture content	385:408	Excreta moisture content, ileal and caecal microbiota and short-chain fatty acid (SCFA) composition and apparent total tract nutrient utilisation were measured.2.
34781802	0	43	theme	Dietary	0:6	arg1	level					42:46	Dietary soluble non-starch polysaccharide level	0:46	Dietary soluble non-starch polysaccharide level	0:46	Dietary soluble non-starch polysaccharide level and xylanase influence the gastrointestinal environment and nutrient utilisation in laying hens.
34781802	2	44	theme	sNSP	251:254	arg1	level					257:261	dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation	208:290	level	257:261	The objective of this study was to examine the influence of dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation on productive performance, viscosity and pH along the gastrointestinal tract in laying hens.
34781802	4	45	theme	laying	561:566	arg1	hens					568:571	Hyline Brown laying hens	548:571	Hyline Brown laying hens (n = 144)	548:581	Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg).
34781802	4	45	theme	laying	561:566	arg1	n = 144					574:580	n = 144	574:580	n = 144	574:580	Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg).
34781802	3	46	theme	SCFA	467:470	arg1	composition					473:483	ileal and caecal microbiota and short-chain fatty acid (SCFA) composition	411:483	composition	473:483	Excreta moisture content, ileal and caecal microbiota and short-chain fatty acid (SCFA) composition and apparent total tract nutrient utilisation were measured.2.
34781802	7	47	theme	moisture	1107:1114	arg1	content					1116:1122	excreta moisture content	1099:1122	excreta moisture content	1099:1122	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	6	48	theme	insoluble	993:1001	arg1	arabinose					1003:1011	insoluble arabinose	993:1011	insoluble arabinose	993:1011	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	8	49	theme	excreta	1468:1474	arg1	pH					1464:1465	caecal pH	1457:1465	caecal pH	1457:1465	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	8	49	theme	excreta	1468:1474	arg1	moisture					1476:1483	excreta moisture	1468:1483	excreta moisture	1468:1483	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	6	50	from	viscosity	934:942	arg1	P < 0.05					1060:1067	P < 0.05	1060:1067	P < 0.05	1060:1067	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	6	50	from	viscosity	934:942	arg1	caeca					1053:1057	the caeca	1049:1057	the caeca (P < 0.05)	1049:1068	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	3	51	theme	acid	461:464	arg1	composition					473:483	ileal and caecal microbiota and short-chain fatty acid (SCFA) composition	411:483	composition	473:483	Excreta moisture content, ileal and caecal microbiota and short-chain fatty acid (SCFA) composition and apparent total tract nutrient utilisation were measured.2.
34781802	7	52	theme	insoluble	1236:1244	arg1	rhamnose					1246:1253	insoluble rhamnose	1236:1253	insoluble rhamnose	1236:1253	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	2	53	theme	study	170:174	arg1	objective					152:160	The objective	148:160	The objective of this study	148:174	The objective of this study was to examine the influence of dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation on productive performance, viscosity and pH along the gastrointestinal tract in laying hens.
34781802	3	54	theme	apparent	489:496	arg1	utilisation					519:529	apparent total tract nutrient utilisation	489:529	apparent total tract nutrient utilisation	489:529	Excreta moisture content, ileal and caecal microbiota and short-chain fatty acid (SCFA) composition and apparent total tract nutrient utilisation were measured.2.
34781802	7	55	theme	high	1079:1082	arg1	sNSP					1084:1087	Feeding high sNSP	1071:1087	Feeding high sNSP	1071:1087	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	11	56	theme	sp	1850:1851	arg1	abundance					1822:1830	caecal abundance	1815:1830	caecal abundance of Bifidobacteria sp	1815:1851	Xylanase application also increased energy retention and insoluble and total NSP degradation, and caecal abundance of Bifidobacteria sp.
34781802	11	56	theme	sp	1850:1851	arg1	retention					1760:1768	energy retention	1753:1768	energy retention	1753:1768	Xylanase application also increased energy retention and insoluble and total NSP degradation, and caecal abundance of Bifidobacteria sp.
34781802	11	56	theme	sp	1850:1851	arg1	degradation					1798:1808	insoluble and total NSP degradation	1774:1808	insoluble and total NSP degradation	1774:1808	Xylanase application also increased energy retention and insoluble and total NSP degradation, and caecal abundance of Bifidobacteria sp.
34781802	7	57	theme	galactose	1222:1230	arg1	concentrations					1300:1313	ileal acetic and lactic acid concentrations	1271:1313	ileal acetic and lactic acid concentrations (P < 0.05)	1271:1324	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	7	57	theme	galactose	1222:1230	arg1	flow					1192:1195	total tract flow	1180:1195	total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose	1180:1264	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	7	57	theme	galactose	1222:1230	arg1	P < 0.05					1316:1323	P < 0.05	1316:1323	P < 0.05	1316:1323	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	7	57	theme	galactose	1222:1230	arg1	content					1116:1122	excreta moisture content	1099:1122	excreta moisture content	1099:1122	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	7	57	theme	galactose	1222:1230	arg1	oligosaccharide					1163:1177	free oligosaccharide	1158:1177	free oligosaccharide	1158:1177	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	7	57	theme	galactose	1222:1230	arg1	tract					1131:1135	total tract energy retention and free oligosaccharide	1125:1177	tract	1131:1135	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	4	58	theme	Hyline	548:553	arg1	hens					568:571	Hyline Brown laying hens	548:571	Hyline Brown laying hens (n = 144)	548:581	Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg).
34781802	4	58	theme	Hyline	548:553	arg1	n = 144					574:580	n = 144	574:580	n = 144	574:580	Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg).
34781802	11	59	theme	energy	1753:1758	arg1	retention					1760:1768	energy retention	1753:1768	energy retention	1753:1768	Xylanase application also increased energy retention and insoluble and total NSP degradation, and caecal abundance of Bifidobacteria sp.
34781802	8	60	theme	arabinose	1502:1510	arg1	flow					1486:1489	flow	1486:1489	flow of soluble arabinose and glucose and insoluble arabinose and xylose	1486:1557	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	8	60	theme	arabinose	1502:1510	arg1	pH					1464:1465	caecal pH	1457:1465	caecal pH	1457:1465	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	6	61	from	concentration	1032:1044	arg1	P < 0.05					1060:1067	P < 0.05	1060:1067	P < 0.05	1060:1067	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	6	61	from	concentration	1032:1044	arg1	caeca					1053:1057	the caeca	1049:1057	the caeca (P < 0.05)	1049:1068	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	11	62	theme	NSP	1794:1796	arg1	degradation					1798:1808	insoluble and total NSP degradation	1774:1808	insoluble and total NSP degradation	1774:1808	Xylanase application also increased energy retention and insoluble and total NSP degradation, and caecal abundance of Bifidobacteria sp.
34781802	7	63	theme	total	1125:1129	arg1	tract					1131:1135	total tract energy retention and free oligosaccharide	1125:1177	tract	1131:1135	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	8	64	theme	glucose	1516:1522	arg1	flow					1486:1489	flow	1486:1489	flow of soluble arabinose and glucose and insoluble arabinose and xylose	1486:1557	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	8	64	theme	glucose	1516:1522	arg1	pH					1464:1465	caecal pH	1457:1465	caecal pH	1457:1465	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	3	65	theme	tract	504:508	arg1	utilisation					519:529	apparent total tract nutrient utilisation	489:529	apparent total tract nutrient utilisation	489:529	Excreta moisture content, ileal and caecal microbiota and short-chain fatty acid (SCFA) composition and apparent total tract nutrient utilisation were measured.2.
34781802	8	66	theme	Lactobacillus	1584:1596	arg1	sp					1598:1599	Lactobacillus sp	1584:1599	Lactobacillus sp	1584:1599	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	7	67	theme	tract	1186:1190	arg1	flow					1192:1195	total tract flow	1180:1195	total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose	1180:1264	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	8	68	theme	insoluble	1528:1536	arg1	arabinose					1538:1546	insoluble arabinose	1528:1546	insoluble arabinose	1528:1546	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	2	69	theme	productive	295:304	arg1	performance					306:316	productive performance	295:316	productive performance	295:316	The objective of this study was to examine the influence of dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation on productive performance, viscosity and pH along the gastrointestinal tract in laying hens.
34781802	13	70	theme	dietary	2066:2072	arg1	level					2079:2083	dietary sNSP level	2066:2083	dietary sNSP level	2066:2083	These results reiterated the ability of xylanase to improve nutrient digestibility and reduce excreta moisture content in laying hens, and highlighted the importance of considering dietary sNSP level in laying hen diets.
34781802	2	71	theme	gastrointestinal	346:361	arg1	tract					363:367	the gastrointestinal tract	342:367	the gastrointestinal tract in laying hens	342:382	The objective of this study was to examine the influence of dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation on productive performance, viscosity and pH along the gastrointestinal tract in laying hens.
34781802	2	72	theme	supplementation	276:290	arg1	influence					195:203	the influence	191:203	the influence of dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation on productive performance, viscosity and pH	191:334	The objective of this study was to examine the influence of dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation on productive performance, viscosity and pH along the gastrointestinal tract in laying hens.
34781802	7	73	theme	egg	1348:1350	arg1	P = 0.058					1364:1372	P = 0.058	1364:1372	P = 0.058	1364:1372	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	7	73	theme	egg	1348:1350	arg1	production					1352:1361	egg production	1348:1361	egg production (P = 0.058)	1348:1373	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	8	74	theme	soluble	1494:1500	arg1	arabinose					1502:1510	soluble arabinose	1494:1510	soluble arabinose	1494:1510	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	6	75	from	flow	985:988	arg1	P < 0.05					1060:1067	P < 0.05	1060:1067	P < 0.05	1060:1067	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	6	75	from	flow	985:988	arg1	caeca					1053:1057	the caeca	1049:1057	the caeca (P < 0.05)	1049:1068	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	11	76	theme	insoluble	1774:1782	arg1	degradation					1798:1808	insoluble and total NSP degradation	1774:1808	insoluble and total NSP degradation	1774:1808	Xylanase application also increased energy retention and insoluble and total NSP degradation, and caecal abundance of Bifidobacteria sp.
34781802	6	77	theme	dietary	903:909	arg1	sNSP					911:914	dietary sNSP	903:914	dietary sNSP	903:914	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	2	78	theme	non-starch	224:233	arg1	level					257:261	dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation	208:290	level	257:261	The objective of this study was to examine the influence of dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation on productive performance, viscosity and pH along the gastrointestinal tract in laying hens.
34781802	4	79	theme	2 × 2	696:700	arg1	arrangement					712:722	a 2 × 2 factorial arrangement	694:722	a 2 × 2 factorial arrangement	694:722	Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg).
34781802	7	80	theme	excreta	1099:1105	arg1	content					1116:1122	excreta moisture content	1099:1122	excreta moisture content	1099:1122	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	13	81	theme	laying	2007:2012	arg1	hens					2014:2017	laying hens	2007:2017	laying hens	2007:2017	These results reiterated the ability of xylanase to improve nutrient digestibility and reduce excreta moisture content in laying hens, and highlighted the importance of considering dietary sNSP level in laying hen diets.
34781802	6	82	theme	tract	979:983	arg1	flow					985:988	total tract flow	973:988	total tract flow of insoluble arabinose	973:1011	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	0	83	from	utilisation	117:127	arg1	hens					139:142	laying hens	132:142	laying hens	132:142	Dietary soluble non-starch polysaccharide level and xylanase influence the gastrointestinal environment and nutrient utilisation in laying hens.
34781802	7	84	theme	acid	1295:1298	arg1	P < 0.05					1316:1323	P < 0.05	1316:1323	P < 0.05	1316:1323	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	7	84	theme	acid	1295:1298	arg1	concentrations					1300:1313	ileal acetic and lactic acid concentrations	1271:1313	ileal acetic and lactic acid concentrations (P < 0.05)	1271:1324	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	0	85	theme	gastrointestinal	75:90	arg1	environment					92:102	the gastrointestinal environment	71:102	the gastrointestinal environment	71:102	Dietary soluble non-starch polysaccharide level and xylanase influence the gastrointestinal environment and nutrient utilisation in laying hens.
34781802	9	86	theme	Bacillus	1663:1670	arg1	sp					1672:1673	Bacillus sp	1663:1673	Bacillus sp	1663:1673	and isobutyric and succinic acid, and ileal concentration of Bacillus sp.
34781802	8	87	theme	reduced	1420:1426	arg1	viscosity					1446:1454	reduced jejunum and ileum viscosity	1420:1454	reduced jejunum and ileum viscosity	1420:1454	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	8	87	theme	reduced	1420:1426	arg1	pH					1464:1465	caecal pH	1457:1465	caecal pH	1457:1465	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	4	88	theme	dietary	672:678	arg1	treatments					680:689	the four wheat-based dietary treatments	651:689	the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg)	651:839	Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg).
34781802	11	89	theme	total	1788:1792	arg1	degradation					1798:1808	insoluble and total NSP degradation	1774:1808	insoluble and total NSP degradation	1774:1808	Xylanase application also increased energy retention and insoluble and total NSP degradation, and caecal abundance of Bifidobacteria sp.
34781802	13	90	theme	excreta	1979:1985	arg1	content					1996:2002	excreta moisture content	1979:2002	excreta moisture content in laying hens	1979:2017	These results reiterated the ability of xylanase to improve nutrient digestibility and reduce excreta moisture content in laying hens, and highlighted the importance of considering dietary sNSP level in laying hen diets.
34781802	4	91	dep	sNSP	753:756	arg1	13.40 g/kg					764:773	High 13.40 g/kg	759:773	High 13.40 g/kg	759:773	Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg).
34781802	4	91	dep	sNSP	753:756	arg1	11.22 g/kg					782:791	Low 11.22 g/kg	778:791	Low 11.22 g/kg	778:791	Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg).
34781802	6	92	theme	total	962:966	arg1	NSP					968:970	total NSP	962:970	total NSP	962:970	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	7	93	theme	energy	1137:1142	arg1	retention					1144:1152	energy retention	1137:1152	energy retention	1137:1152	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	0	94	theme	laying	132:137	arg1	hens					139:142	laying hens	132:142	laying hens	132:142	Dietary soluble non-starch polysaccharide level and xylanase influence the gastrointestinal environment and nutrient utilisation in laying hens.
34781802	13	95	theme	nutrient	1945:1952	arg1	digestibility					1954:1966	nutrient digestibility	1945:1966	nutrient digestibility	1945:1966	These results reiterated the ability of xylanase to improve nutrient digestibility and reduce excreta moisture content in laying hens, and highlighted the importance of considering dietary sNSP level in laying hen diets.
34781802	6	96	theme	acid	1027:1030	arg1	concentration					1032:1044	succinic acid concentration	1018:1044	succinic acid concentration in the caeca (P < 0.05)	1018:1068	Increasing dietary sNSP increased jejunum viscosity, degradability of total NSP, total tract flow of insoluble arabinose, and succinic acid concentration in the caeca (P < 0.05).
34781802	8	97	theme	caecal	1457:1462	arg1	concentration					1567:1579	caecal concentration	1560:1579	caecal concentration of Lactobacillus sp	1560:1599	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	8	97	theme	caecal	1457:1462	arg1	flow					1486:1489	flow	1486:1489	flow of soluble arabinose and glucose and insoluble arabinose and xylose	1486:1557	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	8	97	theme	caecal	1457:1462	arg1	pH					1464:1465	caecal pH	1457:1465	caecal pH	1457:1465	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	8	97	theme	caecal	1457:1462	arg1	moisture					1476:1483	excreta moisture	1468:1483	excreta moisture	1468:1483	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	8	97	theme	caecal	1457:1462	arg1	viscosity					1446:1454	reduced jejunum and ileum viscosity	1420:1454	reduced jejunum and ileum viscosity	1420:1454	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	3	98	theme	fatty	455:459	arg1	composition					473:483	ileal and caecal microbiota and short-chain fatty acid (SCFA) composition	411:483	composition	473:483	Excreta moisture content, ileal and caecal microbiota and short-chain fatty acid (SCFA) composition and apparent total tract nutrient utilisation were measured.2.
34781802	0	99	theme	soluble	8:14	arg1	level					42:46	Dietary soluble non-starch polysaccharide level	0:46	Dietary soluble non-starch polysaccharide level	0:46	Dietary soluble non-starch polysaccharide level and xylanase influence the gastrointestinal environment and nutrient utilisation in laying hens.
34781802	0	100	theme	polysaccharide	27:40	arg1	level					42:46	Dietary soluble non-starch polysaccharide level	0:46	Dietary soluble non-starch polysaccharide level	0:46	Dietary soluble non-starch polysaccharide level and xylanase influence the gastrointestinal environment and nutrient utilisation in laying hens.
34781802	7	101	theme	rhamnose	1246:1253	arg1	concentrations					1300:1313	ileal acetic and lactic acid concentrations	1271:1313	ileal acetic and lactic acid concentrations (P < 0.05)	1271:1324	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	7	101	theme	rhamnose	1246:1253	arg1	flow					1192:1195	total tract flow	1180:1195	total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose	1180:1264	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	7	101	theme	rhamnose	1246:1253	arg1	P < 0.05					1316:1323	P < 0.05	1316:1323	P < 0.05	1316:1323	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	7	101	theme	rhamnose	1246:1253	arg1	content					1116:1122	excreta moisture content	1099:1122	excreta moisture content	1099:1122	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	7	101	theme	rhamnose	1246:1253	arg1	oligosaccharide					1163:1177	free oligosaccharide	1158:1177	free oligosaccharide	1158:1177	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	7	101	theme	rhamnose	1246:1253	arg1	tract					1131:1135	total tract energy retention and free oligosaccharide	1125:1177	tract	1131:1135	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	3	102	theme	Excreta	385:391	arg1	content					402:408	Excreta moisture content	385:408	Excreta moisture content	385:408	Excreta moisture content, ileal and caecal microbiota and short-chain fatty acid (SCFA) composition and apparent total tract nutrient utilisation were measured.2.
34781802	4	103	dep	one	644:646	arg1	to					641:642	to	641:642	to	641:642	Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg).
34781802	3	104	theme	caecal	421:426	arg1	microbiota					428:437	ileal and caecal microbiota and short-chain fatty acid (SCFA) composition	411:483	microbiota	428:437	Excreta moisture content, ileal and caecal microbiota and short-chain fatty acid (SCFA) composition and apparent total tract nutrient utilisation were measured.2.
34781802	11	105	theme	Bifidobacteria	1835:1848	arg1	sp					1850:1851	Bifidobacteria sp	1835:1851	Bifidobacteria sp	1835:1851	Xylanase application also increased energy retention and insoluble and total NSP degradation, and caecal abundance of Bifidobacteria sp.
34781802	7	106	theme	Feeding	1071:1077	arg1	sNSP					1084:1087	Feeding high sNSP	1071:1087	Feeding high sNSP	1071:1087	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	7	107	theme	insoluble	1212:1220	arg1	galactose					1222:1230	soluble and insoluble galactose	1200:1230	soluble and insoluble galactose	1200:1230	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	3	108	theme	ileal	411:415	arg1	microbiota					428:437	ileal and caecal microbiota and short-chain fatty acid (SCFA) composition	411:483	microbiota	428:437	Excreta moisture content, ileal and caecal microbiota and short-chain fatty acid (SCFA) composition and apparent total tract nutrient utilisation were measured.2.
34781802	4	109	theme	Brown	555:559	arg1	hens					568:571	Hyline Brown laying hens	548:571	Hyline Brown laying hens (n = 144)	548:581	Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg).
34781802	4	109	theme	Brown	555:559	arg1	n = 144					574:580	n = 144	574:580	n = 144	574:580	Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg).
34781802	7	110	dep	tract	1131:1135	arg1	retention					1144:1152	energy retention	1137:1152	energy retention	1137:1152	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	2	111	theme	dietary	208:214	arg1	level					257:261	dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation	208:290	level	257:261	The objective of this study was to examine the influence of dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation on productive performance, viscosity and pH along the gastrointestinal tract in laying hens.
34781802	13	112	theme	xylanase	1925:1932	arg1	ability					1914:1920	the ability	1910:1920	the ability of xylanase to improve nutrient digestibility and reduce excreta moisture content in laying hens	1910:2017	These results reiterated the ability of xylanase to improve nutrient digestibility and reduce excreta moisture content in laying hens, and highlighted the importance of considering dietary sNSP level in laying hen diets.
34781802	3	113	theme	total	498:502	arg1	utilisation					519:529	apparent total tract nutrient utilisation	489:529	apparent total tract nutrient utilisation	489:529	Excreta moisture content, ileal and caecal microbiota and short-chain fatty acid (SCFA) composition and apparent total tract nutrient utilisation were measured.2.
34781802	8	114	theme	caecal	1560:1565	arg1	concentration					1567:1579	caecal concentration	1560:1579	caecal concentration of Lactobacillus sp	1560:1599	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	8	114	theme	caecal	1560:1565	arg1	pH					1464:1465	caecal pH	1457:1465	caecal pH	1457:1465	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	7	115	theme	soluble	1200:1206	arg1	galactose					1222:1230	soluble and insoluble galactose	1200:1230	soluble and insoluble galactose	1200:1230	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	7	116	theme	ileal	1271:1275	arg1	P < 0.05					1316:1323	P < 0.05	1316:1323	P < 0.05	1316:1323	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	7	116	theme	ileal	1271:1275	arg1	concentrations					1300:1313	ileal acetic and lactic acid concentrations	1271:1313	ileal acetic and lactic acid concentrations (P < 0.05)	1271:1324	Feeding high sNSP decreased excreta moisture content, total tract energy retention and free oligosaccharide, total tract flow of soluble and insoluble galactose and insoluble rhamnose and fucose, and ileal acetic and lactic acid concentrations (P < 0.05), and tended to reduce egg production (P = 0.058).4.
34781802	4	117	theme	age	623:625	arg1	25 weeks					611:618	25 weeks	611:618	25 weeks of age	611:625	Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg).
34781802	4	118	theme	Low	778:780	arg1	11.22 g/kg					782:791	Low 11.22 g/kg	778:791	Low 11.22 g/kg	778:791	Hyline Brown laying hens (n = 144) were housed individually at 25 weeks of age and allocated to one of the four wheat-based dietary treatments in a 2 × 2 factorial arrangement, consisting of two levels of sNSP (High 13.40 g/kg or Low 11.22 g/kg), with or without xylanase (0 or 12,000 BXU/kg).
34781802	2	119	theme	laying	372:377	arg1	hens					379:382	laying hens	372:382	laying hens	372:382	The objective of this study was to examine the influence of dietary soluble non-starch polysaccharide (sNSP) level and xylanase supplementation on productive performance, viscosity and pH along the gastrointestinal tract in laying hens.
34781802	8	120	theme	sp	1598:1599	arg1	concentration					1567:1579	caecal concentration	1560:1579	caecal concentration of Lactobacillus sp	1560:1599	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	8	120	theme	sp	1598:1599	arg1	pH					1464:1465	caecal pH	1457:1465	caecal pH	1457:1465	Supplementation with xylanase resulted in reduced jejunum and ileum viscosity, caecal pH, excreta moisture, flow of soluble arabinose and glucose and insoluble arabinose and xylose, caecal concentration of Lactobacillus sp.
34781802	13	121	theme	hen	2095:2097	arg1	diets					2099:2103	hen diets	2095:2103	hen diets	2095:2103	These results reiterated the ability of xylanase to improve nutrient digestibility and reduce excreta moisture content in laying hens, and highlighted the importance of considering dietary sNSP level in laying hen diets.
33359257	8	0	theme	antibacterial	1262:1274	arg1	activity					1276:1283	enhanced antibacterial activity	1253:1283	enhanced antibacterial activity	1253:1283	Enhanced osteogenic-related gene expression was observed in the CH/CNC hydrogel scaffold environment than that in the control, indicating their osteogenic potential, in addition to enhanced antibacterial activity.
33359257	8	1	theme	hydrogel	1143:1150	arg1	environment					1161:1171	the CH/CNC hydrogel scaffold environment	1132:1171	the CH/CNC hydrogel scaffold environment	1132:1171	Enhanced osteogenic-related gene expression was observed in the CH/CNC hydrogel scaffold environment than that in the control, indicating their osteogenic potential, in addition to enhanced antibacterial activity.
33359257	9	2	theme	higher	1452:1457	arg1	content					1463:1469	higher CNC content	1452:1469	higher CNC content	1452:1469	Developed composite scaffolds exhibited improved sustained drug release compared to that by pure polymer scaffolds, and this was more sustained in the scaffolds with higher CNC content.
33359257	10	3	theme	antibacterial	1570:1582	arg1	agents					1584:1589	antibacterial agents	1570:1589	antibacterial agents	1570:1589	Therefore, the fabricated scaffolds may have been used in tissue engineering for osteogenesis, as antibacterial agents, and in sustained drug delivery.
33359257	10	3	theme	antibacterial	1570:1582	arg1	scaffolds					1498:1506	the fabricated scaffolds	1483:1506	the fabricated scaffolds	1483:1506	Therefore, the fabricated scaffolds may have been used in tissue engineering for osteogenesis, as antibacterial agents, and in sustained drug delivery.
33359257	0	4	theme	drug	106:109	arg1	delivery					111:118	drug delivery	106:118	drug delivery	106:118	Multifunctional bioactive chitosan/cellulose nanocrystal scaffolds eradicate bacterial growth and sustain drug delivery.
33359257	8	5	theme	CH/CNC	1136:1141	arg1	environment					1161:1171	the CH/CNC hydrogel scaffold environment	1132:1171	the CH/CNC hydrogel scaffold environment	1132:1171	Enhanced osteogenic-related gene expression was observed in the CH/CNC hydrogel scaffold environment than that in the control, indicating their osteogenic potential, in addition to enhanced antibacterial activity.
33359257	5	6	theme	Significant	678:688	arg1	enhancement					690:700	Significant enhancement	678:700	Significant enhancement in the mechanical strength	678:727	Significant enhancement in the mechanical strength was noted in the CH/CNCs as compared to that in pure CH hydrogel scaffolds.
33359257	1	7	theme	regenerative	207:218	arg1	medicine					220:227	regenerative medicine	207:227	regenerative medicine	207:227	Chitosan-based hydrogels have received significant interest in tissue engineering and regenerative medicine applications owing to their superior biocompatibility.
33359257	10	8	theme	drug	1609:1612	arg1	delivery					1614:1621	sustained drug delivery	1599:1621	sustained drug delivery	1599:1621	Therefore, the fabricated scaffolds may have been used in tissue engineering for osteogenesis, as antibacterial agents, and in sustained drug delivery.
33359257	4	9	theme	CH	664:665	arg1	hydrogel					668:675	a chitosan (CH) hydrogel	652:675	a chitosan (CH) hydrogel	652:675	We fabricated a multi-functional hydrogel scaffold of chitosan/CNCs by incorporating different amounts of CNCs into a chitosan (CH) hydrogel.
33359257	3	10	theme	reinforcing	420:430	arg1	nanocrystals					378:389	Cellulose nanocrystals	368:389	Cellulose nanocrystals (CNCs)	368:396	Cellulose nanocrystals (CNCs) are often explored as reinforcing agents to improve the native properties of polymers owing to their superior physicochemical properties.
33359257	3	10	theme	reinforcing	420:430	arg1	agents					432:437	reinforcing agents	420:437	reinforcing agents	420:437	Cellulose nanocrystals (CNCs) are often explored as reinforcing agents to improve the native properties of polymers owing to their superior physicochemical properties.
33359257	6	11	theme	mesenchymal	908:918	arg1	BMSCs					932:936	BMSCs	932:936	BMSCs	932:936	The cytocompatibility of the fabricated scaffolds was monitored in the presence of bone-marrow-derived mesenchymal stem cells (BMSCs).
33359257	6	11	theme	mesenchymal	908:918	arg1	cells					925:929	bone-marrow-derived mesenchymal stem cells	888:929	bone-marrow-derived mesenchymal stem cells (BMSCs)	888:937	The cytocompatibility of the fabricated scaffolds was monitored in the presence of bone-marrow-derived mesenchymal stem cells (BMSCs).
33359257	8	12	dep	activity	1276:1283	arg1	addition					1241:1248	addition	1241:1248	addition	1241:1248	Enhanced osteogenic-related gene expression was observed in the CH/CNC hydrogel scaffold environment than that in the control, indicating their osteogenic potential, in addition to enhanced antibacterial activity.
33359257	9	13	theme	composite	1296:1304	arg1	scaffolds					1306:1314	Developed composite scaffolds	1286:1314	Developed composite scaffolds	1286:1314	Developed composite scaffolds exhibited improved sustained drug release compared to that by pure polymer scaffolds, and this was more sustained in the scaffolds with higher CNC content.
33359257	4	14	theme	multi-functional	552:567	arg1	scaffold					578:585	a multi-functional hydrogel scaffold	550:585	a multi-functional hydrogel scaffold of chitosan/CNCs	550:602	We fabricated a multi-functional hydrogel scaffold of chitosan/CNCs by incorporating different amounts of CNCs into a chitosan (CH) hydrogel.
33359257	8	15	theme	scaffold	1152:1159	arg1	environment					1161:1171	the CH/CNC hydrogel scaffold environment	1132:1171	the CH/CNC hydrogel scaffold environment	1132:1171	Enhanced osteogenic-related gene expression was observed in the CH/CNC hydrogel scaffold environment than that in the control, indicating their osteogenic potential, in addition to enhanced antibacterial activity.
33359257	6	16	theme	bone-marrow-derived	888:906	arg1	BMSCs					932:936	BMSCs	932:936	BMSCs	932:936	The cytocompatibility of the fabricated scaffolds was monitored in the presence of bone-marrow-derived mesenchymal stem cells (BMSCs).
33359257	6	16	theme	bone-marrow-derived	888:906	arg1	cells					925:929	bone-marrow-derived mesenchymal stem cells	888:929	bone-marrow-derived mesenchymal stem cells (BMSCs)	888:937	The cytocompatibility of the fabricated scaffolds was monitored in the presence of bone-marrow-derived mesenchymal stem cells (BMSCs).
33359257	2	17	theme	mechanical	347:356	arg1	strength					358:365	their weak mechanical strength	336:365	their weak mechanical strength	336:365	However, their applications are restricted owing to their weak mechanical strength.
33359257	7	18	theme	cell	949:952	arg1	viability					954:962	Improved cell viability	940:962	Improved cell viability	940:962	Improved cell viability and mineralization were observed with CH/CNC hydrogel scaffolds than those with pure CH hydrogel scaffolds.
33359257	5	19	theme	mechanical	709:718	arg1	strength					720:727	the mechanical strength	705:727	the mechanical strength	705:727	Significant enhancement in the mechanical strength was noted in the CH/CNCs as compared to that in pure CH hydrogel scaffolds.
33359257	7	20	theme	hydrogel	1052:1059	arg1	scaffolds					1061:1069	pure CH hydrogel scaffolds	1044:1069	pure CH hydrogel scaffolds	1044:1069	Improved cell viability and mineralization were observed with CH/CNC hydrogel scaffolds than those with pure CH hydrogel scaffolds.
33359257	2	21	theme	weak	342:345	arg1	strength					358:365	their weak mechanical strength	336:365	their weak mechanical strength	336:365	However, their applications are restricted owing to their weak mechanical strength.
33359257	3	22	theme	superior	499:506	arg1	properties					524:533	their superior physicochemical properties	493:533	their superior physicochemical properties	493:533	Cellulose nanocrystals (CNCs) are often explored as reinforcing agents to improve the native properties of polymers owing to their superior physicochemical properties.
33359257	9	23	from	sustained	1420:1428	arg1	scaffolds					1437:1445	the scaffolds	1433:1445	the scaffolds with higher CNC content	1433:1469	Developed composite scaffolds exhibited improved sustained drug release compared to that by pure polymer scaffolds, and this was more sustained in the scaffolds with higher CNC content.
33359257	1	24	from	interest	172:179	arg1	engineering					191:201	tissue engineering	184:201	tissue engineering	184:201	Chitosan-based hydrogels have received significant interest in tissue engineering and regenerative medicine applications owing to their superior biocompatibility.
33359257	1	24	from	interest	172:179	arg1	medicine					220:227	regenerative medicine	207:227	regenerative medicine	207:227	Chitosan-based hydrogels have received significant interest in tissue engineering and regenerative medicine applications owing to their superior biocompatibility.
33359257	0	25	theme	Multifunctional	0:14	arg1	scaffolds					57:65	Multifunctional bioactive chitosan/cellulose nanocrystal scaffolds	0:65	Multifunctional bioactive chitosan/cellulose nanocrystal scaffolds	0:65	Multifunctional bioactive chitosan/cellulose nanocrystal scaffolds eradicate bacterial growth and sustain drug delivery.
33359257	9	26	theme	pure	1378:1381	arg1	scaffolds					1391:1399	pure polymer scaffolds	1378:1399	pure polymer scaffolds	1378:1399	Developed composite scaffolds exhibited improved sustained drug release compared to that by pure polymer scaffolds, and this was more sustained in the scaffolds with higher CNC content.
33359257	3	27	theme	physicochemical	508:522	arg1	properties					524:533	their superior physicochemical properties	493:533	their superior physicochemical properties	493:533	Cellulose nanocrystals (CNCs) are often explored as reinforcing agents to improve the native properties of polymers owing to their superior physicochemical properties.
33359257	0	28	theme	bioactive	16:24	arg1	scaffolds					57:65	Multifunctional bioactive chitosan/cellulose nanocrystal scaffolds	0:65	Multifunctional bioactive chitosan/cellulose nanocrystal scaffolds	0:65	Multifunctional bioactive chitosan/cellulose nanocrystal scaffolds eradicate bacterial growth and sustain drug delivery.
33359257	4	29	theme	hydrogel	569:576	arg1	scaffold					578:585	a multi-functional hydrogel scaffold	550:585	a multi-functional hydrogel scaffold of chitosan/CNCs	550:602	We fabricated a multi-functional hydrogel scaffold of chitosan/CNCs by incorporating different amounts of CNCs into a chitosan (CH) hydrogel.
33359257	7	30	theme	pure	1044:1047	arg1	scaffolds					1061:1069	pure CH hydrogel scaffolds	1044:1069	pure CH hydrogel scaffolds	1044:1069	Improved cell viability and mineralization were observed with CH/CNC hydrogel scaffolds than those with pure CH hydrogel scaffolds.
33359257	7	31	theme	Improved	940:947	arg1	viability					954:962	Improved cell viability	940:962	Improved cell viability	940:962	Improved cell viability and mineralization were observed with CH/CNC hydrogel scaffolds than those with pure CH hydrogel scaffolds.
33359257	3	32	theme	native	454:459	arg1	properties					461:470	the native properties	450:470	the native properties of polymers owing to their superior physicochemical properties	450:533	Cellulose nanocrystals (CNCs) are often explored as reinforcing agents to improve the native properties of polymers owing to their superior physicochemical properties.
33359257	9	33	theme	CNC	1459:1461	arg1	content					1463:1469	higher CNC content	1452:1469	higher CNC content	1452:1469	Developed composite scaffolds exhibited improved sustained drug release compared to that by pure polymer scaffolds, and this was more sustained in the scaffolds with higher CNC content.
33359257	10	34	theme	tissue	1530:1535	arg1	engineering					1537:1547	tissue engineering	1530:1547	tissue engineering for osteogenesis	1530:1564	Therefore, the fabricated scaffolds may have been used in tissue engineering for osteogenesis, as antibacterial agents, and in sustained drug delivery.
33359257	1	35	theme	Chitosan-based	121:134	arg1	hydrogels					136:144	Chitosan-based hydrogels	121:144	Chitosan-based hydrogels	121:144	Chitosan-based hydrogels have received significant interest in tissue engineering and regenerative medicine applications owing to their superior biocompatibility.
33359257	0	36	theme	nanocrystal	45:55	arg1	scaffolds					57:65	Multifunctional bioactive chitosan/cellulose nanocrystal scaffolds	0:65	Multifunctional bioactive chitosan/cellulose nanocrystal scaffolds	0:65	Multifunctional bioactive chitosan/cellulose nanocrystal scaffolds eradicate bacterial growth and sustain drug delivery.
33359257	6	37	dep	cells	925:929	arg1	the					872:874	the	872:874	the	872:874	The cytocompatibility of the fabricated scaffolds was monitored in the presence of bone-marrow-derived mesenchymal stem cells (BMSCs).
33359257	6	37	dep	cells	925:929	arg1	presence					876:883	presence	876:883	presence	876:883	The cytocompatibility of the fabricated scaffolds was monitored in the presence of bone-marrow-derived mesenchymal stem cells (BMSCs).
33359257	5	38	theme	pure	777:780	arg1	scaffolds					794:802	pure CH hydrogel scaffolds	777:802	pure CH hydrogel scaffolds	777:802	Significant enhancement in the mechanical strength was noted in the CH/CNCs as compared to that in pure CH hydrogel scaffolds.
33359257	8	39	theme	gene	1100:1103	arg1	expression					1105:1114	Enhanced osteogenic-related gene expression	1072:1114	Enhanced osteogenic-related gene expression	1072:1114	Enhanced osteogenic-related gene expression was observed in the CH/CNC hydrogel scaffold environment than that in the control, indicating their osteogenic potential, in addition to enhanced antibacterial activity.
33359257	6	40	theme	stem	920:923	arg1	BMSCs					932:936	BMSCs	932:936	BMSCs	932:936	The cytocompatibility of the fabricated scaffolds was monitored in the presence of bone-marrow-derived mesenchymal stem cells (BMSCs).
33359257	6	40	theme	stem	920:923	arg1	cells					925:929	bone-marrow-derived mesenchymal stem cells	888:929	bone-marrow-derived mesenchymal stem cells (BMSCs)	888:937	The cytocompatibility of the fabricated scaffolds was monitored in the presence of bone-marrow-derived mesenchymal stem cells (BMSCs).
33359257	0	41	theme	chitosan/cellulose	26:43	arg1	scaffolds					57:65	Multifunctional bioactive chitosan/cellulose nanocrystal scaffolds	0:65	Multifunctional bioactive chitosan/cellulose nanocrystal scaffolds	0:65	Multifunctional bioactive chitosan/cellulose nanocrystal scaffolds eradicate bacterial growth and sustain drug delivery.
33359257	5	42	theme	CH	782:783	arg1	scaffolds					794:802	pure CH hydrogel scaffolds	777:802	pure CH hydrogel scaffolds	777:802	Significant enhancement in the mechanical strength was noted in the CH/CNCs as compared to that in pure CH hydrogel scaffolds.
33359257	1	43	dep	engineering	191:201	arg1	applications					229:240	applications	229:240	applications	229:240	Chitosan-based hydrogels have received significant interest in tissue engineering and regenerative medicine applications owing to their superior biocompatibility.
33359257	5	44	from	enhancement	690:700	arg1	strength					720:727	the mechanical strength	705:727	the mechanical strength	705:727	Significant enhancement in the mechanical strength was noted in the CH/CNCs as compared to that in pure CH hydrogel scaffolds.
33359257	1	45	theme	superior	257:264	arg1	biocompatibility					266:281	their superior biocompatibility	251:281	their superior biocompatibility	251:281	Chitosan-based hydrogels have received significant interest in tissue engineering and regenerative medicine applications owing to their superior biocompatibility.
33359257	3	46	theme	polymers	475:482	arg1	properties					461:470	the native properties	450:470	the native properties of polymers owing to their superior physicochemical properties	450:533	Cellulose nanocrystals (CNCs) are often explored as reinforcing agents to improve the native properties of polymers owing to their superior physicochemical properties.
33359257	4	47	theme	chitosan	654:661	arg1	hydrogel					668:675	a chitosan (CH) hydrogel	652:675	a chitosan (CH) hydrogel	652:675	We fabricated a multi-functional hydrogel scaffold of chitosan/CNCs by incorporating different amounts of CNCs into a chitosan (CH) hydrogel.
33359257	5	48	theme	hydrogel	785:792	arg1	scaffolds					794:802	pure CH hydrogel scaffolds	777:802	pure CH hydrogel scaffolds	777:802	Significant enhancement in the mechanical strength was noted in the CH/CNCs as compared to that in pure CH hydrogel scaffolds.
33359257	8	49	theme	osteogenic-related	1081:1098	arg1	expression					1105:1114	Enhanced osteogenic-related gene expression	1072:1114	Enhanced osteogenic-related gene expression	1072:1114	Enhanced osteogenic-related gene expression was observed in the CH/CNC hydrogel scaffold environment than that in the control, indicating their osteogenic potential, in addition to enhanced antibacterial activity.
33359257	9	50	with	scaffolds	1437:1445	arg1	content					1463:1469	higher CNC content	1452:1469	higher CNC content	1452:1469	Developed composite scaffolds exhibited improved sustained drug release compared to that by pure polymer scaffolds, and this was more sustained in the scaffolds with higher CNC content.
33359257	10	51	used	used	1522:1525	arg2	scaffolds					1498:1506	the fabricated scaffolds	1483:1506	the fabricated scaffolds	1483:1506	Therefore, the fabricated scaffolds may have been used in tissue engineering for osteogenesis, as antibacterial agents, and in sustained drug delivery.
33359257	10	51	used	used	1522:1525	arg2	agents					1584:1589	antibacterial agents	1570:1589	antibacterial agents	1570:1589	Therefore, the fabricated scaffolds may have been used in tissue engineering for osteogenesis, as antibacterial agents, and in sustained drug delivery.
33359257	8	52	located	observed	1120:1127	arg1	environment					1161:1171	the CH/CNC hydrogel scaffold environment	1132:1171	the CH/CNC hydrogel scaffold environment	1132:1171	Enhanced osteogenic-related gene expression was observed in the CH/CNC hydrogel scaffold environment than that in the control, indicating their osteogenic potential, in addition to enhanced antibacterial activity.
33359257	8	52	located	observed	1120:1127	arg2	expression					1105:1114	Enhanced osteogenic-related gene expression	1072:1114	Enhanced osteogenic-related gene expression	1072:1114	Enhanced osteogenic-related gene expression was observed in the CH/CNC hydrogel scaffold environment than that in the control, indicating their osteogenic potential, in addition to enhanced antibacterial activity.
33359257	8	53	theme	enhanced	1253:1260	arg1	activity					1276:1283	enhanced antibacterial activity	1253:1283	enhanced antibacterial activity	1253:1283	Enhanced osteogenic-related gene expression was observed in the CH/CNC hydrogel scaffold environment than that in the control, indicating their osteogenic potential, in addition to enhanced antibacterial activity.
33359257	10	54	theme	sustained	1599:1607	arg1	delivery					1614:1621	sustained drug delivery	1599:1621	sustained drug delivery	1599:1621	Therefore, the fabricated scaffolds may have been used in tissue engineering for osteogenesis, as antibacterial agents, and in sustained drug delivery.
33359257	4	55	theme	different	621:629	arg1	CNCs					642:645	CNCs	642:645	CNCs	642:645	We fabricated a multi-functional hydrogel scaffold of chitosan/CNCs by incorporating different amounts of CNCs into a chitosan (CH) hydrogel.
33359257	4	55	theme	different	621:629	arg1	amounts					631:637	different amounts	621:637	different amounts of CNCs	621:645	We fabricated a multi-functional hydrogel scaffold of chitosan/CNCs by incorporating different amounts of CNCs into a chitosan (CH) hydrogel.
33359257	9	56	theme	improved	1326:1333	arg1	release					1350:1356	improved sustained drug release	1326:1356	improved sustained drug release	1326:1356	Developed composite scaffolds exhibited improved sustained drug release compared to that by pure polymer scaffolds, and this was more sustained in the scaffolds with higher CNC content.
33359257	9	57	theme	polymer	1383:1389	arg1	scaffolds					1391:1399	pure polymer scaffolds	1378:1399	pure polymer scaffolds	1378:1399	Developed composite scaffolds exhibited improved sustained drug release compared to that by pure polymer scaffolds, and this was more sustained in the scaffolds with higher CNC content.
33359257	6	58	theme	scaffolds	845:853	arg1	cytocompatibility					809:825	The cytocompatibility	805:825	The cytocompatibility of the fabricated scaffolds	805:853	The cytocompatibility of the fabricated scaffolds was monitored in the presence of bone-marrow-derived mesenchymal stem cells (BMSCs).
33359257	7	59	theme	CH	1049:1050	arg1	scaffolds					1061:1069	pure CH hydrogel scaffolds	1044:1069	pure CH hydrogel scaffolds	1044:1069	Improved cell viability and mineralization were observed with CH/CNC hydrogel scaffolds than those with pure CH hydrogel scaffolds.
33359257	0	60	theme	bacterial	77:85	arg1	growth					87:92	bacterial growth	77:92	bacterial growth	77:92	Multifunctional bioactive chitosan/cellulose nanocrystal scaffolds eradicate bacterial growth and sustain drug delivery.
33359257	4	61	theme	CNCs	642:645	arg1	CNCs					642:645	CNCs	642:645	CNCs	642:645	We fabricated a multi-functional hydrogel scaffold of chitosan/CNCs by incorporating different amounts of CNCs into a chitosan (CH) hydrogel.
33359257	4	61	theme	CNCs	642:645	arg1	amounts					631:637	different amounts	621:637	different amounts of CNCs	621:645	We fabricated a multi-functional hydrogel scaffold of chitosan/CNCs by incorporating different amounts of CNCs into a chitosan (CH) hydrogel.
33359257	8	62	theme	osteogenic	1216:1225	arg1	potential					1227:1235	their osteogenic potential	1210:1235	their osteogenic potential	1210:1235	Enhanced osteogenic-related gene expression was observed in the CH/CNC hydrogel scaffold environment than that in the control, indicating their osteogenic potential, in addition to enhanced antibacterial activity.
33359257	6	63	theme	fabricated	834:843	arg1	scaffolds					845:853	the fabricated scaffolds	830:853	the fabricated scaffolds	830:853	The cytocompatibility of the fabricated scaffolds was monitored in the presence of bone-marrow-derived mesenchymal stem cells (BMSCs).
33359257	1	64	theme	significant	160:170	arg1	interest					172:179	significant interest	160:179	significant interest in tissue engineering and regenerative medicine applications	160:240	Chitosan-based hydrogels have received significant interest in tissue engineering and regenerative medicine applications owing to their superior biocompatibility.
33359257	7	65	theme	hydrogel	1009:1016	arg1	scaffolds					1018:1026	CH/CNC hydrogel scaffolds	1002:1026	CH/CNC hydrogel scaffolds than those with pure CH hydrogel scaffolds	1002:1069	Improved cell viability and mineralization were observed with CH/CNC hydrogel scaffolds than those with pure CH hydrogel scaffolds.
33359257	9	66	theme	drug	1345:1348	arg1	release					1350:1356	improved sustained drug release	1326:1356	improved sustained drug release	1326:1356	Developed composite scaffolds exhibited improved sustained drug release compared to that by pure polymer scaffolds, and this was more sustained in the scaffolds with higher CNC content.
33359257	8	67	theme	Enhanced	1072:1079	arg1	expression					1105:1114	Enhanced osteogenic-related gene expression	1072:1114	Enhanced osteogenic-related gene expression	1072:1114	Enhanced osteogenic-related gene expression was observed in the CH/CNC hydrogel scaffold environment than that in the control, indicating their osteogenic potential, in addition to enhanced antibacterial activity.
33359257	3	68	theme	Cellulose	368:376	arg1	CNCs					392:395	CNCs	392:395	CNCs	392:395	Cellulose nanocrystals (CNCs) are often explored as reinforcing agents to improve the native properties of polymers owing to their superior physicochemical properties.
33359257	3	68	theme	Cellulose	368:376	arg1	nanocrystals					378:389	Cellulose nanocrystals	368:389	Cellulose nanocrystals (CNCs)	368:396	Cellulose nanocrystals (CNCs) are often explored as reinforcing agents to improve the native properties of polymers owing to their superior physicochemical properties.
33359257	3	68	theme	Cellulose	368:376	arg1	agents					432:437	reinforcing agents	420:437	reinforcing agents	420:437	Cellulose nanocrystals (CNCs) are often explored as reinforcing agents to improve the native properties of polymers owing to their superior physicochemical properties.
33359257	9	69	theme	sustained	1335:1343	arg1	release					1350:1356	improved sustained drug release	1326:1356	improved sustained drug release	1326:1356	Developed composite scaffolds exhibited improved sustained drug release compared to that by pure polymer scaffolds, and this was more sustained in the scaffolds with higher CNC content.
33359257	6	70	link	bone-marrow-derived	888:906	arg1	BMSCs					932:936	BMSCs	932:936	BMSCs	932:936	The cytocompatibility of the fabricated scaffolds was monitored in the presence of bone-marrow-derived mesenchymal stem cells (BMSCs).
33359257	6	70	link	bone-marrow-derived	888:906	arg1	cells					925:929	bone-marrow-derived mesenchymal stem cells	888:929	bone-marrow-derived mesenchymal stem cells (BMSCs)	888:937	The cytocompatibility of the fabricated scaffolds was monitored in the presence of bone-marrow-derived mesenchymal stem cells (BMSCs).
33359257	10	71	theme	fabricated	1487:1496	arg1	agents					1584:1589	antibacterial agents	1570:1589	antibacterial agents	1570:1589	Therefore, the fabricated scaffolds may have been used in tissue engineering for osteogenesis, as antibacterial agents, and in sustained drug delivery.
33359257	10	71	theme	fabricated	1487:1496	arg1	scaffolds					1498:1506	the fabricated scaffolds	1483:1506	the fabricated scaffolds	1483:1506	Therefore, the fabricated scaffolds may have been used in tissue engineering for osteogenesis, as antibacterial agents, and in sustained drug delivery.
33359257	1	72	theme	tissue	184:189	arg1	engineering					191:201	tissue engineering	184:201	tissue engineering	184:201	Chitosan-based hydrogels have received significant interest in tissue engineering and regenerative medicine applications owing to their superior biocompatibility.
33359257	7	73	theme	CH/CNC	1002:1007	arg1	scaffolds					1018:1026	CH/CNC hydrogel scaffolds	1002:1026	CH/CNC hydrogel scaffolds than those with pure CH hydrogel scaffolds	1002:1069	Improved cell viability and mineralization were observed with CH/CNC hydrogel scaffolds than those with pure CH hydrogel scaffolds.
33359257	4	74	theme	chitosan/CNCs	590:602	arg1	scaffold					578:585	a multi-functional hydrogel scaffold	550:585	a multi-functional hydrogel scaffold of chitosan/CNCs	550:602	We fabricated a multi-functional hydrogel scaffold of chitosan/CNCs by incorporating different amounts of CNCs into a chitosan (CH) hydrogel.
33359257	9	75	from	scaffolds	1437:1445	arg1	sustained					1420:1428	sustained	1420:1428	sustained	1420:1428	Developed composite scaffolds exhibited improved sustained drug release compared to that by pure polymer scaffolds, and this was more sustained in the scaffolds with higher CNC content.
33359257	9	76	theme	Developed	1286:1294	arg1	scaffolds					1306:1314	Developed composite scaffolds	1286:1314	Developed composite scaffolds	1286:1314	Developed composite scaffolds exhibited improved sustained drug release compared to that by pure polymer scaffolds, and this was more sustained in the scaffolds with higher CNC content.
32174400	6	0	theme	In	726:727	arg1	experiments					735:745	In vitro experiments	726:745	In vitro experiments	726:745	In vitro experiments showed that human umbilical vein endothelial cells can successfully attach to the printed scaffold and maintain high viability during the course of study.
32174400	1	1	theme	organs	139:144	arg1	printing					115:122	3D printing	112:122	3D printing of tissues and organs	112:144	Bioink plays a major role in 3D printing of tissues and organs.
32174400	8	2	theme	biological	1145:1154	arg1	functionality					1156:1168	favorable biological functionality	1135:1168	favorable biological functionality	1135:1168	In conclusion, the results of this study demonstrate that 3D printed Albumen/NaAlg composite bioinks with favorable biological functionality hold a great potential in tissue and organ engineering.
32174400	4	3	theme	3D	482:483	arg1	bioink					504:509	3D printing composite bioink	482:509	3D printing composite bioink	482:509	The experimental conditions necessary for 3D printing composite bioink were optimized by changing different concentration ratios of Albumen/NaAlg and their various physicochemical properties were studied.
32174400	5	4	theme	structural	649:658	arg1	characteristics					660:674	The structural characteristics	645:674	The structural characteristics of the 3D printed scaffold	645:701	The structural characteristics of the 3D printed scaffold was also investigated.
32174400	6	5	theme	printed	829:835	arg1	scaffold					837:844	the printed scaffold	825:844	the printed scaffold	825:844	In vitro experiments showed that human umbilical vein endothelial cells can successfully attach to the printed scaffold and maintain high viability during the course of study.
32174400	8	6	dep	tissue	1196:1201	arg1	engineering					1213:1223	engineering	1213:1223	engineering	1213:1223	In conclusion, the results of this study demonstrate that 3D printed Albumen/NaAlg composite bioinks with favorable biological functionality hold a great potential in tissue and organ engineering.
32174400	4	7	theme	concentration	548:560	arg1	ratios					562:567	different concentration ratios	538:567	different concentration ratios of Albumen/NaAlg	538:584	The experimental conditions necessary for 3D printing composite bioink were optimized by changing different concentration ratios of Albumen/NaAlg and their various physicochemical properties were studied.
32174400	3	8	theme	cellular	325:332	arg1	compatibility					334:346	cellular compatibility	325:346	cellular compatibility	325:346	In this study, we demonstrate the printability and cellular compatibility of composite bioink consists of sodium alginate (NaAlg) and egg white, also called albumen.
32174400	8	9	theme	study	1064:1068	arg1	results					1048:1054	the results	1044:1054	the results of this study	1044:1068	In conclusion, the results of this study demonstrate that 3D printed Albumen/NaAlg composite bioinks with favorable biological functionality hold a great potential in tissue and organ engineering.
32174400	7	10	theme	printed	1011:1017	arg1	scaffold					1019:1026	the printed scaffold	1007:1026	the printed scaffold	1007:1026	Interestingly, vascular sprouting and neovascular network formation was observed inbetween fibers within the printed scaffold.
32174400	4	11	theme	Albumen/NaAlg	572:584	arg1	ratios					562:567	different concentration ratios	538:567	different concentration ratios of Albumen/NaAlg	538:584	The experimental conditions necessary for 3D printing composite bioink were optimized by changing different concentration ratios of Albumen/NaAlg and their various physicochemical properties were studied.
32174400	3	12	dep	consists	368:375	arg1	called					424:429	called	424:429	called albumen	424:437	In this study, we demonstrate the printability and cellular compatibility of composite bioink consists of sodium alginate (NaAlg) and egg white, also called albumen.
32174400	2	13	theme	clinical	252:259	arg1	translation					261:271	its clinical translation	248:271	its clinical translation	248:271	Alginate is a widely used component for bioinks but its cellular responses are limited, which limits its clinical translation.
32174400	6	14	attach	attach	815:820	arg1	scaffold					837:844	the printed scaffold	825:844	the printed scaffold	825:844	In vitro experiments showed that human umbilical vein endothelial cells can successfully attach to the printed scaffold and maintain high viability during the course of study.
32174400	6	14	attach	attach	815:820	arg2	cells					792:796	human umbilical vein endothelial cells	759:796	human umbilical vein endothelial cells	759:796	In vitro experiments showed that human umbilical vein endothelial cells can successfully attach to the printed scaffold and maintain high viability during the course of study.
32174400	7	15	theme	inbetween	983:991	arg1	fibers					993:998	inbetween fibers	983:998	inbetween fibers	983:998	Interestingly, vascular sprouting and neovascular network formation was observed inbetween fibers within the printed scaffold.
32174400	7	16	located	observed	974:981	arg1	scaffold					1019:1026	the printed scaffold	1007:1026	the printed scaffold	1007:1026	Interestingly, vascular sprouting and neovascular network formation was observed inbetween fibers within the printed scaffold.
32174400	7	16	located	observed	974:981	arg2	sprouting					926:934	vascular sprouting	917:934	vascular sprouting	917:934	Interestingly, vascular sprouting and neovascular network formation was observed inbetween fibers within the printed scaffold.
32174400	7	16	located	observed	974:981	arg2	formation					960:968	neovascular network formation	940:968	neovascular network formation	940:968	Interestingly, vascular sprouting and neovascular network formation was observed inbetween fibers within the printed scaffold.
32174400	4	17	theme	experimental	444:455	arg1	conditions					457:466	The experimental conditions	440:466	The experimental conditions necessary for 3D printing composite bioink	440:509	The experimental conditions necessary for 3D printing composite bioink were optimized by changing different concentration ratios of Albumen/NaAlg and their various physicochemical properties were studied.
32174400	0	18	theme	vascular	10:17	arg1	bioink					53:58	vascular supportive albumen-rich composite bioink	10:58	vascular supportive albumen-rich composite bioink for organ 3D printing	10:80	Designing vascular supportive albumen-rich composite bioink for organ 3D printing.
32174400	8	19	theme	printed	1090:1096	arg1	bioinks					1122:1128	3D printed Albumen/NaAlg composite bioinks	1087:1128	3D printed Albumen/NaAlg composite bioinks with favorable biological functionality	1087:1168	In conclusion, the results of this study demonstrate that 3D printed Albumen/NaAlg composite bioinks with favorable biological functionality hold a great potential in tissue and organ engineering.
32174400	8	20	theme	favorable	1135:1143	arg1	functionality					1156:1168	favorable biological functionality	1135:1168	favorable biological functionality	1135:1168	In conclusion, the results of this study demonstrate that 3D printed Albumen/NaAlg composite bioinks with favorable biological functionality hold a great potential in tissue and organ engineering.
32174400	8	21	theme	3D	1087:1088	arg1	bioinks					1122:1128	3D printed Albumen/NaAlg composite bioinks	1087:1128	3D printed Albumen/NaAlg composite bioinks with favorable biological functionality	1087:1168	In conclusion, the results of this study demonstrate that 3D printed Albumen/NaAlg composite bioinks with favorable biological functionality hold a great potential in tissue and organ engineering.
32174400	7	22	theme	neovascular	940:950	arg1	formation					960:968	neovascular network formation	940:968	neovascular network formation	940:968	Interestingly, vascular sprouting and neovascular network formation was observed inbetween fibers within the printed scaffold.
32174400	0	23	theme	albumen-rich	30:41	arg1	bioink					53:58	vascular supportive albumen-rich composite bioink	10:58	vascular supportive albumen-rich composite bioink for organ 3D printing	10:80	Designing vascular supportive albumen-rich composite bioink for organ 3D printing.
32174400	8	24	theme	composite	1112:1120	arg1	bioinks					1122:1128	3D printed Albumen/NaAlg composite bioinks	1087:1128	3D printed Albumen/NaAlg composite bioinks with favorable biological functionality	1087:1168	In conclusion, the results of this study demonstrate that 3D printed Albumen/NaAlg composite bioinks with favorable biological functionality hold a great potential in tissue and organ engineering.
32174400	6	25	theme	endothelial	780:790	arg1	cells					792:796	human umbilical vein endothelial cells	759:796	human umbilical vein endothelial cells	759:796	In vitro experiments showed that human umbilical vein endothelial cells can successfully attach to the printed scaffold and maintain high viability during the course of study.
32174400	1	26	theme	major	98:102	arg1	role					104:107	a major role	96:107	a major role	96:107	Bioink plays a major role in 3D printing of tissues and organs.
32174400	3	27	theme	composite	351:359	arg1	bioink					361:366	composite bioink	351:366	composite bioink	351:366	In this study, we demonstrate the printability and cellular compatibility of composite bioink consists of sodium alginate (NaAlg) and egg white, also called albumen.
32174400	4	28	theme	different	538:546	arg1	ratios					562:567	different concentration ratios	538:567	different concentration ratios of Albumen/NaAlg	538:584	The experimental conditions necessary for 3D printing composite bioink were optimized by changing different concentration ratios of Albumen/NaAlg and their various physicochemical properties were studied.
32174400	8	29	theme	Albumen/NaAlg	1098:1110	arg1	bioinks					1122:1128	3D printed Albumen/NaAlg composite bioinks	1087:1128	3D printed Albumen/NaAlg composite bioinks with favorable biological functionality	1087:1168	In conclusion, the results of this study demonstrate that 3D printed Albumen/NaAlg composite bioinks with favorable biological functionality hold a great potential in tissue and organ engineering.
32174400	8	30	with	bioinks	1122:1128	arg1	functionality					1156:1168	favorable biological functionality	1135:1168	favorable biological functionality	1135:1168	In conclusion, the results of this study demonstrate that 3D printed Albumen/NaAlg composite bioinks with favorable biological functionality hold a great potential in tissue and organ engineering.
32174400	8	31	from	potential	1183:1191	arg1	organ					1207:1211	organ	1207:1211	organ	1207:1211	In conclusion, the results of this study demonstrate that 3D printed Albumen/NaAlg composite bioinks with favorable biological functionality hold a great potential in tissue and organ engineering.
32174400	8	31	from	potential	1183:1191	arg1	tissue					1196:1201	tissue	1196:1201	tissue	1196:1201	In conclusion, the results of this study demonstrate that 3D printed Albumen/NaAlg composite bioinks with favorable biological functionality hold a great potential in tissue and organ engineering.
32174400	3	32	theme	white	412:416	arg1	egg					408:410	egg white	408:416	egg white	408:416	In this study, we demonstrate the printability and cellular compatibility of composite bioink consists of sodium alginate (NaAlg) and egg white, also called albumen.
32174400	0	33	theme	composite	43:51	arg1	bioink					53:58	vascular supportive albumen-rich composite bioink	10:58	vascular supportive albumen-rich composite bioink for organ 3D printing	10:80	Designing vascular supportive albumen-rich composite bioink for organ 3D printing.
32174400	4	34	theme	composite	494:502	arg1	bioink					504:509	3D printing composite bioink	482:509	3D printing composite bioink	482:509	The experimental conditions necessary for 3D printing composite bioink were optimized by changing different concentration ratios of Albumen/NaAlg and their various physicochemical properties were studied.
32174400	2	35	theme	cellular	203:210	arg1	responses					212:220	its cellular responses	199:220	its cellular responses	199:220	Alginate is a widely used component for bioinks but its cellular responses are limited, which limits its clinical translation.
32174400	6	36	theme	vein	775:778	arg1	cells					792:796	human umbilical vein endothelial cells	759:796	human umbilical vein endothelial cells	759:796	In vitro experiments showed that human umbilical vein endothelial cells can successfully attach to the printed scaffold and maintain high viability during the course of study.
32174400	0	37	theme	organ	64:68	arg1	printing					73:80	organ 3D printing	64:80	organ 3D printing	64:80	Designing vascular supportive albumen-rich composite bioink for organ 3D printing.
32174400	4	38	theme	printing	485:492	arg1	bioink					504:509	3D printing composite bioink	482:509	3D printing composite bioink	482:509	The experimental conditions necessary for 3D printing composite bioink were optimized by changing different concentration ratios of Albumen/NaAlg and their various physicochemical properties were studied.
32174400	3	39	dep	printability	308:319	arg1	the					304:306	the	304:306	the	304:306	In this study, we demonstrate the printability and cellular compatibility of composite bioink consists of sodium alginate (NaAlg) and egg white, also called albumen.
32174400	6	40	theme	study	895:899	arg1	course					885:890	the course	881:890	the course of study	881:899	In vitro experiments showed that human umbilical vein endothelial cells can successfully attach to the printed scaffold and maintain high viability during the course of study.
32174400	3	41	theme	sodium	380:385	arg1	NaAlg					397:401	NaAlg	397:401	NaAlg	397:401	In this study, we demonstrate the printability and cellular compatibility of composite bioink consists of sodium alginate (NaAlg) and egg white, also called albumen.
32174400	3	41	theme	sodium	380:385	arg1	alginate					387:394	sodium alginate	380:394	sodium alginate (NaAlg)	380:402	In this study, we demonstrate the printability and cellular compatibility of composite bioink consists of sodium alginate (NaAlg) and egg white, also called albumen.
32174400	5	42	theme	3D	683:684	arg1	scaffold					694:701	the 3D printed scaffold	679:701	the 3D printed scaffold	679:701	The structural characteristics of the 3D printed scaffold was also investigated.
32174400	6	43	theme	high	859:862	arg1	viability					864:872	high viability	859:872	high viability	859:872	In vitro experiments showed that human umbilical vein endothelial cells can successfully attach to the printed scaffold and maintain high viability during the course of study.
32174400	6	44	theme	human	759:763	arg1	cells					792:796	human umbilical vein endothelial cells	759:796	human umbilical vein endothelial cells	759:796	In vitro experiments showed that human umbilical vein endothelial cells can successfully attach to the printed scaffold and maintain high viability during the course of study.
32174400	1	45	theme	3D	112:113	arg1	printing					115:122	3D printing	112:122	3D printing of tissues and organs	112:144	Bioink plays a major role in 3D printing of tissues and organs.
32174400	7	46	theme	network	952:958	arg1	formation					960:968	neovascular network formation	940:968	neovascular network formation	940:968	Interestingly, vascular sprouting and neovascular network formation was observed inbetween fibers within the printed scaffold.
32174400	5	47	theme	printed	686:692	arg1	scaffold					694:701	the 3D printed scaffold	679:701	the 3D printed scaffold	679:701	The structural characteristics of the 3D printed scaffold was also investigated.
32174400	6	48	dep	In	726:727	arg1	vitro					729:733	vitro	729:733	vitro	729:733	In vitro experiments showed that human umbilical vein endothelial cells can successfully attach to the printed scaffold and maintain high viability during the course of study.
32174400	6	49	theme	umbilical	765:773	arg1	cells					792:796	human umbilical vein endothelial cells	759:796	human umbilical vein endothelial cells	759:796	In vitro experiments showed that human umbilical vein endothelial cells can successfully attach to the printed scaffold and maintain high viability during the course of study.
32174400	5	50	theme	scaffold	694:701	arg1	characteristics					660:674	The structural characteristics	645:674	The structural characteristics of the 3D printed scaffold	645:701	The structural characteristics of the 3D printed scaffold was also investigated.
32174400	8	51	theme	great	1177:1181	arg1	potential					1183:1191	a great potential	1175:1191	a great potential in tissue and organ engineering	1175:1223	In conclusion, the results of this study demonstrate that 3D printed Albumen/NaAlg composite bioinks with favorable biological functionality hold a great potential in tissue and organ engineering.
32174400	2	52	theme	used	168:171	arg1	component					173:181	a widely used component	159:181	a widely used component for bioinks	159:193	Alginate is a widely used component for bioinks but its cellular responses are limited, which limits its clinical translation.
32174400	2	52	theme	used	168:171	arg1	Alginate					147:154	Alginate	147:154	Alginate	147:154	Alginate is a widely used component for bioinks but its cellular responses are limited, which limits its clinical translation.
32174400	1	53	theme	tissues	127:133	arg1	printing					115:122	3D printing	112:122	3D printing of tissues and organs	112:144	Bioink plays a major role in 3D printing of tissues and organs.
32174400	7	54	theme	vascular	917:924	arg1	sprouting					926:934	vascular sprouting	917:934	vascular sprouting	917:934	Interestingly, vascular sprouting and neovascular network formation was observed inbetween fibers within the printed scaffold.
32174400	4	55	theme	physicochemical	604:618	arg1	properties					620:629	their various physicochemical properties	590:629	their various physicochemical properties	590:629	The experimental conditions necessary for 3D printing composite bioink were optimized by changing different concentration ratios of Albumen/NaAlg and their various physicochemical properties were studied.
32174400	0	56	theme	3D	70:71	arg1	printing					73:80	organ 3D printing	64:80	organ 3D printing	64:80	Designing vascular supportive albumen-rich composite bioink for organ 3D printing.
32174400	4	57	theme	necessary	468:476	arg1	conditions					457:466	The experimental conditions	440:466	The experimental conditions necessary for 3D printing composite bioink	440:509	The experimental conditions necessary for 3D printing composite bioink were optimized by changing different concentration ratios of Albumen/NaAlg and their various physicochemical properties were studied.
32174400	0	58	theme	supportive	19:28	arg1	bioink					53:58	vascular supportive albumen-rich composite bioink	10:58	vascular supportive albumen-rich composite bioink for organ 3D printing	10:80	Designing vascular supportive albumen-rich composite bioink for organ 3D printing.
32174400	3	59	theme	bioink	361:366	arg1	printability					308:319	printability	308:319	printability	308:319	In this study, we demonstrate the printability and cellular compatibility of composite bioink consists of sodium alginate (NaAlg) and egg white, also called albumen.
32174400	3	59	theme	bioink	361:366	arg1	compatibility					334:346	cellular compatibility	325:346	cellular compatibility	325:346	In this study, we demonstrate the printability and cellular compatibility of composite bioink consists of sodium alginate (NaAlg) and egg white, also called albumen.
32174400	4	60	theme	various	596:602	arg1	properties					620:629	their various physicochemical properties	590:629	their various physicochemical properties	590:629	The experimental conditions necessary for 3D printing composite bioink were optimized by changing different concentration ratios of Albumen/NaAlg and their various physicochemical properties were studied.
33645130	12	0	theme	IL-1β	1734:1738	arg1	expressions					1719:1729	the relative gene expressions	1701:1729	the relative gene expressions of IL-1β, IL-6 and MCP-1	1701:1754	The relative expressions of IL-10 and IFN-γ were up-regulated to varying degrees, while the relative gene expressions of IL-1β, IL-6 and MCP-1 were down-regulated to different degrees.
33645130	15	1	theme	better	2187:2192	arg1	activity					2204:2211	a better antiviral activity	2185:2211	a better antiviral activity	2185:2211	Compared with the prescription of MXF, AP had a better antiviral activity.
33645130	14	2	theme	cytokines	2128:2136	arg1	expression					2101:2110	the expression	2097:2110	the expression of inflammatory cytokines	2097:2136	AP had a good anti-influenza virus activity in vitro, and could protect mice with kidney Yang deficiency syndrome by reducing the viral load in lung tissue, decreasing inflammation damage in lung tissue, and regulating the expression of inflammatory cytokines.
33645130	14	3	with	mice	1950:1953	arg1	syndrome					1983:1990	kidney Yang deficiency syndrome	1960:1990	kidney Yang deficiency syndrome	1960:1990	AP had a good anti-influenza virus activity in vitro, and could protect mice with kidney Yang deficiency syndrome by reducing the viral load in lung tissue, decreasing inflammation damage in lung tissue, and regulating the expression of inflammatory cytokines.
33645130	3	4	theme	cell	505:508	arg1	system					531:536	The cell Real-time monitoring system	501:536	The cell Real-time monitoring system	501:536	The cell Real-time monitoring system and Reed-Muench model were adopted to evaluate the antiviral activity of AP in vitro.
33645130	1	5	theme	H1	240:241	arg1	virus					256:260	H1 N1 influenza virus	240:260	H1 N1 influenza virus	240:260	In this paper, Asarum polysaccharides(AP) were extracted, and its composition was analyzed to study the activity against H1 N1 influenza virus in vitro and its intervention effect on mice with kidney Yang deficiency syndrome.
33645130	12	6	theme	relative	1705:1712	arg1	expressions					1719:1729	the relative gene expressions	1701:1729	the relative gene expressions of IL-1β, IL-6 and MCP-1	1701:1754	The relative expressions of IL-10 and IFN-γ were up-regulated to varying degrees, while the relative gene expressions of IL-1β, IL-6 and MCP-1 were down-regulated to different degrees.
33645130	7	7	from	content	1065:1071	arg1	Asarum					1082:1087	Asarum	1082:1087	Asarum	1082:1087	The content of AP in Asarum was 25.22%, and the protein content was 0.8%.
33645130	6	8	theme	M	999:999	arg1	gene					1001:1004	M gene	999:1004	M gene of H1 N1 influenza virus and cytokines	999:1043	Real-time PCR was used to detect the relative expression of M gene of H1 N1 influenza virus and cytokines in lung tissue.
33645130	15	9	contain	had	2181:2183	arg1	AP					2178:2179	AP	2178:2179	AP	2178:2179	Compared with the prescription of MXF, AP had a better antiviral activity.
33645130	15	9	contain	had	2181:2183	arg2	activity					2204:2211	a better antiviral activity	2185:2211	a better antiviral activity	2185:2211	Compared with the prescription of MXF, AP had a better antiviral activity.
33645130	5	10	theme	AP	786:787	arg1	group					789:793	AP group	786:793	AP group	786:793	MXF group and AP group were treated with clinical equivalent doses of 1.8 g·kg~(-1)·d~(-1) and 0.077 g·kg~(-1)·d~(-1) respectively, once a day for 6 consecutive days.
33645130	14	11	theme	viral	2008:2012	arg1	load					2014:2017	the viral load	2004:2017	the viral load in lung tissue	2004:2032	AP had a good anti-influenza virus activity in vitro, and could protect mice with kidney Yang deficiency syndrome by reducing the viral load in lung tissue, decreasing inflammation damage in lung tissue, and regulating the expression of inflammatory cytokines.
33645130	3	12	theme	monitoring	520:529	arg1	system					531:536	The cell Real-time monitoring system	501:536	The cell Real-time monitoring system	501:536	The cell Real-time monitoring system and Reed-Muench model were adopted to evaluate the antiviral activity of AP in vitro.
33645130	6	13	theme	lung	1048:1051	arg1	tissue					1053:1058	lung tissue	1048:1058	lung tissue	1048:1058	Real-time PCR was used to detect the relative expression of M gene of H1 N1 influenza virus and cytokines in lung tissue.
33645130	1	14	from	activity	223:230	arg1	mice					302:305	mice	302:305	mice with kidney Yang deficiency syndrome	302:342	In this paper, Asarum polysaccharides(AP) were extracted, and its composition was analyzed to study the activity against H1 N1 influenza virus in vitro and its intervention effect on mice with kidney Yang deficiency syndrome.
33645130	9	15	theme	TI	1261:1262	arg1	values					1264:1269	TI values	1261:1269	TI values of Tamiflu, MXF and AP	1261:1292	TI values of Tamiflu, MXF and AP were 30.00, 8.06 and 10.33, respectively.
33645130	6	16	theme	cytokines	1035:1043	arg1	gene					1001:1004	M gene	999:1004	M gene of H1 N1 influenza virus and cytokines	999:1043	Real-time PCR was used to detect the relative expression of M gene of H1 N1 influenza virus and cytokines in lung tissue.
33645130	4	17	theme	deficiency	659:668	arg1	syndrome					670:677	kidney Yang deficiency syndrome	647:677	kidney Yang deficiency syndrome	647:677	And the mouse model of kidney Yang deficiency syndrome was established in vivo to compare the efficacy of Mahuang Xixin Fuzi Decoction(MXF) and AP.
33645130	10	18	theme	AP	1361:1362	arg1	doses					1352:1356	Three different doses	1336:1356	Three different doses of AP	1336:1362	Three different doses of AP could significantly reduce the concentration of virus in supernatant.
33645130	12	19	theme	MCP-1	1750:1754	arg1	expressions					1719:1729	the relative gene expressions	1701:1729	the relative gene expressions of IL-1β, IL-6 and MCP-1	1701:1754	The relative expressions of IL-10 and IFN-γ were up-regulated to varying degrees, while the relative gene expressions of IL-1β, IL-6 and MCP-1 were down-regulated to different degrees.
33645130	14	20	from	damage	2059:2064	arg1	tissue					2074:2079	lung tissue	2069:2079	lung tissue	2069:2079	AP had a good anti-influenza virus activity in vitro, and could protect mice with kidney Yang deficiency syndrome by reducing the viral load in lung tissue, decreasing inflammation damage in lung tissue, and regulating the expression of inflammatory cytokines.
33645130	6	21	theme	virus	1025:1029	arg1	gene					1001:1004	M gene	999:1004	M gene of H1 N1 influenza virus and cytokines	999:1043	Real-time PCR was used to detect the relative expression of M gene of H1 N1 influenza virus and cytokines in lung tissue.
33645130	12	22	theme	different	1779:1787	arg1	degrees					1789:1795	different degrees	1779:1795	different degrees	1779:1795	The relative expressions of IL-10 and IFN-γ were up-regulated to varying degrees, while the relative gene expressions of IL-1β, IL-6 and MCP-1 were down-regulated to different degrees.
33645130	3	23	theme	antiviral	589:597	arg1	activity					599:606	the antiviral activity	585:606	the antiviral activity of AP	585:612	The cell Real-time monitoring system and Reed-Muench model were adopted to evaluate the antiviral activity of AP in vitro.
33645130	5	24	theme	g·kg~	846:850	arg1	-1					859:860	-1	859:860	-1	859:860	MXF group and AP group were treated with clinical equivalent doses of 1.8 g·kg~(-1)·d~(-1) and 0.077 g·kg~(-1)·d~(-1) respectively, once a day for 6 consecutive days.
33645130	5	24	theme	g·kg~	846:850	arg1	·d~					855:857	1.8 g·kg~(-1)·d~	842:857	1.8 g·kg~(-1)·d~(-1)	842:861	MXF group and AP group were treated with clinical equivalent doses of 1.8 g·kg~(-1)·d~(-1) and 0.077 g·kg~(-1)·d~(-1) respectively, once a day for 6 consecutive days.
33645130	6	25	theme	N1	1012:1013	arg1	virus					1025:1029	H1 N1 influenza virus	1009:1029	H1 N1 influenza virus	1009:1029	Real-time PCR was used to detect the relative expression of M gene of H1 N1 influenza virus and cytokines in lung tissue.
33645130	12	26	theme	IL-6	1741:1744	arg1	expressions					1719:1729	the relative gene expressions	1701:1729	the relative gene expressions of IL-1β, IL-6 and MCP-1	1701:1754	The relative expressions of IL-10 and IFN-γ were up-regulated to varying degrees, while the relative gene expressions of IL-1β, IL-6 and MCP-1 were down-regulated to different degrees.
33645130	14	27	theme	deficiency	1972:1981	arg1	syndrome					1983:1990	kidney Yang deficiency syndrome	1960:1990	kidney Yang deficiency syndrome	1960:1990	AP had a good anti-influenza virus activity in vitro, and could protect mice with kidney Yang deficiency syndrome by reducing the viral load in lung tissue, decreasing inflammation damage in lung tissue, and regulating the expression of inflammatory cytokines.
33645130	11	28	theme	MXF	1480:1482	arg1	group					1484:1488	MXF group	1480:1488	MXF group	1480:1488	Compared with the model mice, lung indexes of MXF group and AP group decreased significantly(P&lt;0.05), and the relative expression of M gene decreased significantly(P&lt;0.05).
33645130	0	29	theme	virus	62:66	arg1	infection					68:76	H1N1 influenza virus infection	47:76	H1N1 influenza virus infection	47:76	[Protective effect of Asarum polysaccharide on H1N1 influenza virus infection and expression of inflammatory factors].
33645130	6	30	used	used	957:960	arg2	PCR					949:951	Real-time PCR	939:951	Real-time PCR	939:951	Real-time PCR was used to detect the relative expression of M gene of H1 N1 influenza virus and cytokines in lung tissue.
33645130	1	31	theme	Asarum	134:139	arg1	AP					157:158	AP	157:158	AP	157:158	In this paper, Asarum polysaccharides(AP) were extracted, and its composition was analyzed to study the activity against H1 N1 influenza virus in vitro and its intervention effect on mice with kidney Yang deficiency syndrome.
33645130	1	31	theme	Asarum	134:139	arg1	polysaccharides					141:155	Asarum polysaccharides	134:155	Asarum polysaccharides(AP)	134:159	In this paper, Asarum polysaccharides(AP) were extracted, and its composition was analyzed to study the activity against H1 N1 influenza virus in vitro and its intervention effect on mice with kidney Yang deficiency syndrome.
33645130	1	32	with	mice	302:305	arg1	syndrome					335:342	kidney Yang deficiency syndrome	312:342	kidney Yang deficiency syndrome	312:342	In this paper, Asarum polysaccharides(AP) were extracted, and its composition was analyzed to study the activity against H1 N1 influenza virus in vitro and its intervention effect on mice with kidney Yang deficiency syndrome.
33645130	1	33	dep	in	262:263	arg1	vitro					265:269	vitro	265:269	vitro	265:269	In this paper, Asarum polysaccharides(AP) were extracted, and its composition was analyzed to study the activity against H1 N1 influenza virus in vitro and its intervention effect on mice with kidney Yang deficiency syndrome.
33645130	4	34	theme	kidney	647:652	arg1	syndrome					670:677	kidney Yang deficiency syndrome	647:677	kidney Yang deficiency syndrome	647:677	And the mouse model of kidney Yang deficiency syndrome was established in vivo to compare the efficacy of Mahuang Xixin Fuzi Decoction(MXF) and AP.
33645130	14	35	theme	kidney	1960:1965	arg1	syndrome					1983:1990	kidney Yang deficiency syndrome	1960:1990	kidney Yang deficiency syndrome	1960:1990	AP had a good anti-influenza virus activity in vitro, and could protect mice with kidney Yang deficiency syndrome by reducing the viral load in lung tissue, decreasing inflammation damage in lung tissue, and regulating the expression of inflammatory cytokines.
33645130	5	36	theme	equivalent	822:831	arg1	doses					833:837	clinical equivalent doses	813:837	clinical equivalent doses of 1.8 g·kg~(-1)·d~(-1) and 0.077 g·kg~(-1)·d~(-1)	813:888	MXF group and AP group were treated with clinical equivalent doses of 1.8 g·kg~(-1)·d~(-1) and 0.077 g·kg~(-1)·d~(-1) respectively, once a day for 6 consecutive days.
33645130	3	37	theme	AP	611:612	arg1	activity					599:606	the antiviral activity	585:606	the antiviral activity of AP	585:612	The cell Real-time monitoring system and Reed-Muench model were adopted to evaluate the antiviral activity of AP in vitro.
33645130	11	38	dep	P&lt	1601:1604	arg1	0.05					1606:1609	0.05	1606:1609	0.05	1606:1609	Compared with the model mice, lung indexes of MXF group and AP group decreased significantly(P&lt;0.05), and the relative expression of M gene decreased significantly(P&lt;0.05).
33645130	11	39	theme	group	1497:1501	arg1	indexes					1469:1475	lung indexes	1464:1475	lung indexes of MXF group and AP group	1464:1501	Compared with the model mice, lung indexes of MXF group and AP group decreased significantly(P&lt;0.05), and the relative expression of M gene decreased significantly(P&lt;0.05).
33645130	5	40	theme	consecutive	921:931	arg1	days					933:936	6 consecutive days	919:936	6 consecutive days	919:936	MXF group and AP group were treated with clinical equivalent doses of 1.8 g·kg~(-1)·d~(-1) and 0.077 g·kg~(-1)·d~(-1) respectively, once a day for 6 consecutive days.
33645130	2	41	theme	monosaccharide	460:473	arg1	composition					475:485	its monosaccharide composition	456:485	its monosaccharide composition	456:485	AP was prepared by the strategy of water extraction and alcohol precipitation, the content was determined, and its monosaccharide composition was analyzed.
33645130	10	42	theme	virus	1412:1416	arg1	concentration					1395:1407	the concentration	1391:1407	the concentration of virus in supernatant	1391:1431	Three different doses of AP could significantly reduce the concentration of virus in supernatant.
33645130	0	43	theme	factors	109:115	arg1	expression					82:91	expression	82:91	expression of inflammatory factors	82:115	[Protective effect of Asarum polysaccharide on H1N1 influenza virus infection and expression of inflammatory factors].
33645130	0	43	theme	factors	109:115	arg1	infection					68:76	H1N1 influenza virus infection	47:76	H1N1 influenza virus infection	47:76	[Protective effect of Asarum polysaccharide on H1N1 influenza virus infection and expression of inflammatory factors].
33645130	1	44	theme	deficiency	324:333	arg1	syndrome					335:342	kidney Yang deficiency syndrome	312:342	kidney Yang deficiency syndrome	312:342	In this paper, Asarum polysaccharides(AP) were extracted, and its composition was analyzed to study the activity against H1 N1 influenza virus in vitro and its intervention effect on mice with kidney Yang deficiency syndrome.
33645130	2	45	theme	alcohol	401:407	arg1	precipitation					409:421	alcohol precipitation	401:421	alcohol precipitation	401:421	AP was prepared by the strategy of water extraction and alcohol precipitation, the content was determined, and its monosaccharide composition was analyzed.
33645130	0	46	theme	[Protective	0:10	arg1	effect					12:17	[Protective effect	0:17	[Protective effect of Asarum polysaccharide on H1N1 influenza virus infection and expression of inflammatory factors	0:115	[Protective effect of Asarum polysaccharide on H1N1 influenza virus infection and expression of inflammatory factors].
33645130	1	47	theme	N1	243:244	arg1	virus					256:260	H1 N1 influenza virus	240:260	H1 N1 influenza virus	240:260	In this paper, Asarum polysaccharides(AP) were extracted, and its composition was analyzed to study the activity against H1 N1 influenza virus in vitro and its intervention effect on mice with kidney Yang deficiency syndrome.
33645130	2	48	theme	extraction	386:395	arg1	strategy					368:375	the strategy	364:375	the strategy of water extraction and alcohol precipitation	364:421	AP was prepared by the strategy of water extraction and alcohol precipitation, the content was determined, and its monosaccharide composition was analyzed.
33645130	5	49	theme	·d~	855:857	arg1	doses					833:837	clinical equivalent doses	813:837	clinical equivalent doses of 1.8 g·kg~(-1)·d~(-1) and 0.077 g·kg~(-1)·d~(-1)	813:888	MXF group and AP group were treated with clinical equivalent doses of 1.8 g·kg~(-1)·d~(-1) and 0.077 g·kg~(-1)·d~(-1) respectively, once a day for 6 consecutive days.
33645130	14	50	theme	virus	1907:1911	arg1	activity					1913:1920	a good anti-influenza virus activity	1885:1920	a good anti-influenza virus activity	1885:1920	AP had a good anti-influenza virus activity in vitro, and could protect mice with kidney Yang deficiency syndrome by reducing the viral load in lung tissue, decreasing inflammation damage in lung tissue, and regulating the expression of inflammatory cytokines.
33645130	0	51	theme	polysaccharide	29:42	arg1	effect					12:17	[Protective effect	0:17	[Protective effect of Asarum polysaccharide on H1N1 influenza virus infection and expression of inflammatory factors	0:115	[Protective effect of Asarum polysaccharide on H1N1 influenza virus infection and expression of inflammatory factors].
33645130	5	52	theme	·d~	882:884	arg1	doses					833:837	clinical equivalent doses	813:837	clinical equivalent doses of 1.8 g·kg~(-1)·d~(-1) and 0.077 g·kg~(-1)·d~(-1)	813:888	MXF group and AP group were treated with clinical equivalent doses of 1.8 g·kg~(-1)·d~(-1) and 0.077 g·kg~(-1)·d~(-1) respectively, once a day for 6 consecutive days.
33645130	10	53	from	concentration	1395:1407	arg1	supernatant					1421:1431	supernatant	1421:1431	supernatant	1421:1431	Three different doses of AP could significantly reduce the concentration of virus in supernatant.
33645130	11	54	theme	M	1570:1570	arg1	gene					1572:1575	M gene	1570:1575	M gene	1570:1575	Compared with the model mice, lung indexes of MXF group and AP group decreased significantly(P&lt;0.05), and the relative expression of M gene decreased significantly(P&lt;0.05).
33645130	0	55	theme	H1N1	47:50	arg1	infection					68:76	H1N1 influenza virus infection	47:76	H1N1 influenza virus infection	47:76	[Protective effect of Asarum polysaccharide on H1N1 influenza virus infection and expression of inflammatory factors].
33645130	13	56	theme	TNF-α	1860:1864	arg1	expression					1846:1855	the expression	1842:1855	the expression of TNF-α	1842:1864	In addition, AP could significantly enhance the expression of TNF-α(P&lt;0.01).
33645130	7	57	theme	AP	1076:1077	arg1	%					1098:1098	25.22%	1093:1098	25.22%	1093:1098	The content of AP in Asarum was 25.22%, and the protein content was 0.8%.
33645130	7	57	theme	AP	1076:1077	arg1	content					1065:1071	The content	1061:1071	The content of AP in Asarum	1061:1087	The content of AP in Asarum was 25.22%, and the protein content was 0.8%.
33645130	14	58	theme	anti-influenza	1892:1905	arg1	activity					1913:1920	a good anti-influenza virus activity	1885:1920	a good anti-influenza virus activity	1885:1920	AP had a good anti-influenza virus activity in vitro, and could protect mice with kidney Yang deficiency syndrome by reducing the viral load in lung tissue, decreasing inflammation damage in lung tissue, and regulating the expression of inflammatory cytokines.
33645130	11	59	theme	lung	1464:1467	arg1	indexes					1469:1475	lung indexes	1464:1475	lung indexes of MXF group and AP group	1464:1501	Compared with the model mice, lung indexes of MXF group and AP group decreased significantly(P&lt;0.05), and the relative expression of M gene decreased significantly(P&lt;0.05).
33645130	4	60	theme	Decoction	749:757	arg1	efficacy					718:725	the efficacy	714:725	the efficacy of Mahuang Xixin Fuzi Decoction(MXF) and AP	714:769	And the mouse model of kidney Yang deficiency syndrome was established in vivo to compare the efficacy of Mahuang Xixin Fuzi Decoction(MXF) and AP.
33645130	0	61	theme	influenza	52:60	arg1	infection					68:76	H1N1 influenza virus infection	47:76	H1N1 influenza virus infection	47:76	[Protective effect of Asarum polysaccharide on H1N1 influenza virus infection and expression of inflammatory factors].
33645130	14	62	theme	inflammation	2046:2057	arg1	damage					2059:2064	inflammation damage	2046:2064	inflammation damage in lung tissue	2046:2079	AP had a good anti-influenza virus activity in vitro, and could protect mice with kidney Yang deficiency syndrome by reducing the viral load in lung tissue, decreasing inflammation damage in lung tissue, and regulating the expression of inflammatory cytokines.
33645130	15	63	theme	antiviral	2194:2202	arg1	activity					2204:2211	a better antiviral activity	2185:2211	a better antiviral activity	2185:2211	Compared with the prescription of MXF, AP had a better antiviral activity.
33645130	14	64	theme	inflammatory	2115:2126	arg1	cytokines					2128:2136	inflammatory cytokines	2115:2136	inflammatory cytokines	2115:2136	AP had a good anti-influenza virus activity in vitro, and could protect mice with kidney Yang deficiency syndrome by reducing the viral load in lung tissue, decreasing inflammation damage in lung tissue, and regulating the expression of inflammatory cytokines.
33645130	6	65	theme	gene	1001:1004	arg1	expression					985:994	the relative expression	972:994	the relative expression of M gene of H1 N1 influenza virus and cytokines in lung tissue	972:1058	Real-time PCR was used to detect the relative expression of M gene of H1 N1 influenza virus and cytokines in lung tissue.
33645130	14	66	contain	had	1881:1883	arg1	AP					1878:1879	AP	1878:1879	AP	1878:1879	AP had a good anti-influenza virus activity in vitro, and could protect mice with kidney Yang deficiency syndrome by reducing the viral load in lung tissue, decreasing inflammation damage in lung tissue, and regulating the expression of inflammatory cytokines.
33645130	14	66	contain	had	1881:1883	arg2	activity					1913:1920	a good anti-influenza virus activity	1885:1920	a good anti-influenza virus activity	1885:1920	AP had a good anti-influenza virus activity in vitro, and could protect mice with kidney Yang deficiency syndrome by reducing the viral load in lung tissue, decreasing inflammation damage in lung tissue, and regulating the expression of inflammatory cytokines.
33645130	14	67	theme	lung	2022:2025	arg1	tissue					2027:2032	lung tissue	2022:2032	lung tissue	2022:2032	AP had a good anti-influenza virus activity in vitro, and could protect mice with kidney Yang deficiency syndrome by reducing the viral load in lung tissue, decreasing inflammation damage in lung tissue, and regulating the expression of inflammatory cytokines.
33645130	4	68	theme	AP	768:769	arg1	efficacy					718:725	the efficacy	714:725	the efficacy of Mahuang Xixin Fuzi Decoction(MXF) and AP	714:769	And the mouse model of kidney Yang deficiency syndrome was established in vivo to compare the efficacy of Mahuang Xixin Fuzi Decoction(MXF) and AP.
33645130	6	69	theme	relative	976:983	arg1	expression					985:994	the relative expression	972:994	the relative expression of M gene of H1 N1 influenza virus and cytokines in lung tissue	972:1058	Real-time PCR was used to detect the relative expression of M gene of H1 N1 influenza virus and cytokines in lung tissue.
33645130	10	70	theme	different	1342:1350	arg1	doses					1352:1356	Three different doses	1336:1356	Three different doses of AP	1336:1362	Three different doses of AP could significantly reduce the concentration of virus in supernatant.
33645130	3	71	theme	Real-time	510:518	arg1	system					531:536	The cell Real-time monitoring system	501:536	The cell Real-time monitoring system	501:536	The cell Real-time monitoring system and Reed-Muench model were adopted to evaluate the antiviral activity of AP in vitro.
33645130	12	72	theme	varying	1678:1684	arg1	degrees					1686:1692	varying degrees	1678:1692	varying degrees	1678:1692	The relative expressions of IL-10 and IFN-γ were up-regulated to varying degrees, while the relative gene expressions of IL-1β, IL-6 and MCP-1 were down-regulated to different degrees.
33645130	13	73	dep	P&lt	1866:1869	arg1	0.01					1871:1874	0.01	1871:1874	0.01	1871:1874	In addition, AP could significantly enhance the expression of TNF-α(P&lt;0.01).
33645130	14	74	from	load	2014:2017	arg1	tissue					2027:2032	lung tissue	2022:2032	lung tissue	2022:2032	AP had a good anti-influenza virus activity in vitro, and could protect mice with kidney Yang deficiency syndrome by reducing the viral load in lung tissue, decreasing inflammation damage in lung tissue, and regulating the expression of inflammatory cytokines.
33645130	9	75	theme	AP	1291:1292	arg1	values					1264:1269	TI values	1261:1269	TI values of Tamiflu, MXF and AP	1261:1292	TI values of Tamiflu, MXF and AP were 30.00, 8.06 and 10.33, respectively.
33645130	12	76	theme	IL-10	1641:1645	arg1	expressions					1626:1636	The relative expressions	1613:1636	The relative expressions of IL-10 and IFN-γ	1613:1655	The relative expressions of IL-10 and IFN-γ were up-regulated to varying degrees, while the relative gene expressions of IL-1β, IL-6 and MCP-1 were down-regulated to different degrees.
33645130	11	77	theme	relative	1547:1554	arg1	expression					1556:1565	the relative expression	1543:1565	the relative expression of M gene	1543:1575	Compared with the model mice, lung indexes of MXF group and AP group decreased significantly(P&lt;0.05), and the relative expression of M gene decreased significantly(P&lt;0.05).
33645130	3	78	theme	Reed-Muench	542:552	arg1	model					554:558	Reed-Muench model	542:558	Reed-Muench model	542:558	The cell Real-time monitoring system and Reed-Muench model were adopted to evaluate the antiviral activity of AP in vitro.
33645130	12	79	theme	IFN-γ	1651:1655	arg1	expressions					1626:1636	The relative expressions	1613:1636	The relative expressions of IL-10 and IFN-γ	1613:1655	The relative expressions of IL-10 and IFN-γ were up-regulated to varying degrees, while the relative gene expressions of IL-1β, IL-6 and MCP-1 were down-regulated to different degrees.
33645130	4	80	theme	Yang	654:657	arg1	syndrome					670:677	kidney Yang deficiency syndrome	647:677	kidney Yang deficiency syndrome	647:677	And the mouse model of kidney Yang deficiency syndrome was established in vivo to compare the efficacy of Mahuang Xixin Fuzi Decoction(MXF) and AP.
33645130	11	81	dep	decreased	1503:1511	arg1	P&lt					1527:1530	P&lt	1527:1530	P&lt	1527:1530	Compared with the model mice, lung indexes of MXF group and AP group decreased significantly(P&lt;0.05), and the relative expression of M gene decreased significantly(P&lt;0.05).
33645130	13	82	dep	enhance	1834:1840	arg1	P&lt					1866:1869	P&lt	1866:1869	P&lt	1866:1869	In addition, AP could significantly enhance the expression of TNF-α(P&lt;0.01).
33645130	6	83	theme	influenza	1015:1023	arg1	virus					1025:1029	H1 N1 influenza virus	1009:1029	H1 N1 influenza virus	1009:1029	Real-time PCR was used to detect the relative expression of M gene of H1 N1 influenza virus and cytokines in lung tissue.
33645130	4	84	theme	syndrome	670:677	arg1	model					638:642	the mouse model	628:642	the mouse model of kidney Yang deficiency syndrome	628:677	And the mouse model of kidney Yang deficiency syndrome was established in vivo to compare the efficacy of Mahuang Xixin Fuzi Decoction(MXF) and AP.
33645130	11	85	theme	AP	1494:1495	arg1	group					1497:1501	AP group	1494:1501	AP group	1494:1501	Compared with the model mice, lung indexes of MXF group and AP group decreased significantly(P&lt;0.05), and the relative expression of M gene decreased significantly(P&lt;0.05).
33645130	9	86	theme	MXF	1283:1285	arg1	values					1264:1269	TI values	1261:1269	TI values of Tamiflu, MXF and AP	1261:1292	TI values of Tamiflu, MXF and AP were 30.00, 8.06 and 10.33, respectively.
33645130	6	87	theme	H1	1009:1010	arg1	virus					1025:1029	H1 N1 influenza virus	1009:1029	H1 N1 influenza virus	1009:1029	Real-time PCR was used to detect the relative expression of M gene of H1 N1 influenza virus and cytokines in lung tissue.
33645130	4	88	theme	mouse	632:636	arg1	model					638:642	the mouse model	628:642	the mouse model of kidney Yang deficiency syndrome	628:677	And the mouse model of kidney Yang deficiency syndrome was established in vivo to compare the efficacy of Mahuang Xixin Fuzi Decoction(MXF) and AP.
33645130	14	89	theme	Yang	1967:1970	arg1	syndrome					1983:1990	kidney Yang deficiency syndrome	1960:1990	kidney Yang deficiency syndrome	1960:1990	AP had a good anti-influenza virus activity in vitro, and could protect mice with kidney Yang deficiency syndrome by reducing the viral load in lung tissue, decreasing inflammation damage in lung tissue, and regulating the expression of inflammatory cytokines.
33645130	11	90	theme	group	1484:1488	arg1	indexes					1469:1475	lung indexes	1464:1475	lung indexes of MXF group and AP group	1464:1501	Compared with the model mice, lung indexes of MXF group and AP group decreased significantly(P&lt;0.05), and the relative expression of M gene decreased significantly(P&lt;0.05).
33645130	12	91	theme	relative	1617:1624	arg1	expressions					1626:1636	The relative expressions	1613:1636	The relative expressions of IL-10 and IFN-γ	1613:1655	The relative expressions of IL-10 and IFN-γ were up-regulated to varying degrees, while the relative gene expressions of IL-1β, IL-6 and MCP-1 were down-regulated to different degrees.
33645130	5	92	theme	clinical	813:820	arg1	doses					833:837	clinical equivalent doses	813:837	clinical equivalent doses of 1.8 g·kg~(-1)·d~(-1) and 0.077 g·kg~(-1)·d~(-1)	813:888	MXF group and AP group were treated with clinical equivalent doses of 1.8 g·kg~(-1)·d~(-1) and 0.077 g·kg~(-1)·d~(-1) respectively, once a day for 6 consecutive days.
33645130	1	93	theme	kidney	312:317	arg1	syndrome					335:342	kidney Yang deficiency syndrome	312:342	kidney Yang deficiency syndrome	312:342	In this paper, Asarum polysaccharides(AP) were extracted, and its composition was analyzed to study the activity against H1 N1 influenza virus in vitro and its intervention effect on mice with kidney Yang deficiency syndrome.
33645130	0	94	from	effect	12:17	arg1	expression					82:91	expression	82:91	expression of inflammatory factors	82:115	[Protective effect of Asarum polysaccharide on H1N1 influenza virus infection and expression of inflammatory factors].
33645130	0	94	from	effect	12:17	arg1	infection					68:76	H1N1 influenza virus infection	47:76	H1N1 influenza virus infection	47:76	[Protective effect of Asarum polysaccharide on H1N1 influenza virus infection and expression of inflammatory factors].
33645130	0	95	theme	inflammatory	96:107	arg1	factors					109:115	inflammatory factors	96:115	inflammatory factors	96:115	[Protective effect of Asarum polysaccharide on H1N1 influenza virus infection and expression of inflammatory factors].
33645130	1	96	theme	Yang	319:322	arg1	syndrome					335:342	kidney Yang deficiency syndrome	312:342	kidney Yang deficiency syndrome	312:342	In this paper, Asarum polysaccharides(AP) were extracted, and its composition was analyzed to study the activity against H1 N1 influenza virus in vitro and its intervention effect on mice with kidney Yang deficiency syndrome.
33645130	7	97	theme	protein	1109:1115	arg1	%					1132:1132	0.8%	1129:1132	0.8%	1129:1132	The content of AP in Asarum was 25.22%, and the protein content was 0.8%.
33645130	7	97	theme	protein	1109:1115	arg1	content					1117:1123	the protein content	1105:1123	the protein content	1105:1123	The content of AP in Asarum was 25.22%, and the protein content was 0.8%.
33645130	15	98	theme	MXF	2173:2175	arg1	prescription					2157:2168	the prescription	2153:2168	the prescription of MXF	2153:2175	Compared with the prescription of MXF, AP had a better antiviral activity.
33645130	11	99	dep	decreased	1577:1585	arg1	P&lt					1601:1604	P&lt	1601:1604	P&lt;0.05	1601:1609	Compared with the model mice, lung indexes of MXF group and AP group decreased significantly(P&lt;0.05), and the relative expression of M gene decreased significantly(P&lt;0.05).
33645130	0	100	theme	Asarum	22:27	arg1	polysaccharide					29:42	Asarum polysaccharide	22:42	Asarum polysaccharide	22:42	[Protective effect of Asarum polysaccharide on H1N1 influenza virus infection and expression of inflammatory factors].
33645130	1	101	theme	influenza	246:254	arg1	virus					256:260	H1 N1 influenza virus	240:260	H1 N1 influenza virus	240:260	In this paper, Asarum polysaccharides(AP) were extracted, and its composition was analyzed to study the activity against H1 N1 influenza virus in vitro and its intervention effect on mice with kidney Yang deficiency syndrome.
33645130	2	102	theme	water	380:384	arg1	extraction					386:395	water extraction	380:395	water extraction	380:395	AP was prepared by the strategy of water extraction and alcohol precipitation, the content was determined, and its monosaccharide composition was analyzed.
33645130	11	103	theme	model	1452:1456	arg1	mice					1458:1461	the model mice	1448:1461	the model mice	1448:1461	Compared with the model mice, lung indexes of MXF group and AP group decreased significantly(P&lt;0.05), and the relative expression of M gene decreased significantly(P&lt;0.05).
33645130	8	104	theme	monosaccharide	1143:1156	arg1	L-rhamnose					1188:1197	L-rhamnose	1188:1197	L-rhamnose	1188:1197	And the monosaccharide composition was identified as L-rhamnose, D-arabinose, D-xylose, D-glucose, D-galactose and D-mannose.
33645130	8	104	theme	monosaccharide	1143:1156	arg1	D-glucose					1223:1231	D-glucose	1223:1231	D-glucose	1223:1231	And the monosaccharide composition was identified as L-rhamnose, D-arabinose, D-xylose, D-glucose, D-galactose and D-mannose.
33645130	8	104	theme	monosaccharide	1143:1156	arg1	D-galactose					1234:1244	D-galactose	1234:1244	D-galactose	1234:1244	And the monosaccharide composition was identified as L-rhamnose, D-arabinose, D-xylose, D-glucose, D-galactose and D-mannose.
33645130	8	104	theme	monosaccharide	1143:1156	arg1	composition					1158:1168	the monosaccharide composition	1139:1168	the monosaccharide composition	1139:1168	And the monosaccharide composition was identified as L-rhamnose, D-arabinose, D-xylose, D-glucose, D-galactose and D-mannose.
33645130	8	104	theme	monosaccharide	1143:1156	arg1	D-arabinose					1200:1210	D-arabinose	1200:1210	D-arabinose	1200:1210	And the monosaccharide composition was identified as L-rhamnose, D-arabinose, D-xylose, D-glucose, D-galactose and D-mannose.
33645130	8	104	theme	monosaccharide	1143:1156	arg1	D-xylose					1213:1220	D-xylose	1213:1220	D-xylose	1213:1220	And the monosaccharide composition was identified as L-rhamnose, D-arabinose, D-xylose, D-glucose, D-galactose and D-mannose.
33645130	8	104	theme	monosaccharide	1143:1156	arg1	D-mannose					1250:1258	D-mannose	1250:1258	D-mannose	1250:1258	And the monosaccharide composition was identified as L-rhamnose, D-arabinose, D-xylose, D-glucose, D-galactose and D-mannose.
33645130	6	105	from	expression	985:994	arg1	tissue					1053:1058	lung tissue	1048:1058	lung tissue	1048:1058	Real-time PCR was used to detect the relative expression of M gene of H1 N1 influenza virus and cytokines in lung tissue.
33645130	1	106	theme	in	262:263	arg1	activity					223:230	the activity	219:230	the activity against H1 N1 influenza virus in vitro	219:269	In this paper, Asarum polysaccharides(AP) were extracted, and its composition was analyzed to study the activity against H1 N1 influenza virus in vitro and its intervention effect on mice with kidney Yang deficiency syndrome.
33645130	5	107	theme	MXF	772:774	arg1	group					776:780	MXF group	772:780	MXF group	772:780	MXF group and AP group were treated with clinical equivalent doses of 1.8 g·kg~(-1)·d~(-1) and 0.077 g·kg~(-1)·d~(-1) respectively, once a day for 6 consecutive days.
33645130	1	108	from	effect	292:297	arg1	mice					302:305	mice	302:305	mice with kidney Yang deficiency syndrome	302:342	In this paper, Asarum polysaccharides(AP) were extracted, and its composition was analyzed to study the activity against H1 N1 influenza virus in vitro and its intervention effect on mice with kidney Yang deficiency syndrome.
33645130	12	109	theme	gene	1714:1717	arg1	expressions					1719:1729	the relative gene expressions	1701:1729	the relative gene expressions of IL-1β, IL-6 and MCP-1	1701:1754	The relative expressions of IL-10 and IFN-γ were up-regulated to varying degrees, while the relative gene expressions of IL-1β, IL-6 and MCP-1 were down-regulated to different degrees.
33645130	2	110	theme	precipitation	409:421	arg1	strategy					368:375	the strategy	364:375	the strategy of water extraction and alcohol precipitation	364:421	AP was prepared by the strategy of water extraction and alcohol precipitation, the content was determined, and its monosaccharide composition was analyzed.
33645130	11	111	theme	gene	1572:1575	arg1	expression					1556:1565	the relative expression	1543:1565	the relative expression of M gene	1543:1575	Compared with the model mice, lung indexes of MXF group and AP group decreased significantly(P&lt;0.05), and the relative expression of M gene decreased significantly(P&lt;0.05).
33645130	1	112	theme	intervention	279:290	arg1	effect					292:297	its intervention effect	275:297	its intervention effect on mice with kidney Yang deficiency syndrome	275:342	In this paper, Asarum polysaccharides(AP) were extracted, and its composition was analyzed to study the activity against H1 N1 influenza virus in vitro and its intervention effect on mice with kidney Yang deficiency syndrome.
33645130	5	113	theme	0.077	867:871	arg1	·d~					882:884	0.077 g·kg~(-1)·d~	867:884	0.077 g·kg~(-1)·d~(-1)	867:888	MXF group and AP group were treated with clinical equivalent doses of 1.8 g·kg~(-1)·d~(-1) and 0.077 g·kg~(-1)·d~(-1) respectively, once a day for 6 consecutive days.
33645130	5	113	theme	0.077	867:871	arg1	-1					886:887	-1	886:887	-1	886:887	MXF group and AP group were treated with clinical equivalent doses of 1.8 g·kg~(-1)·d~(-1) and 0.077 g·kg~(-1)·d~(-1) respectively, once a day for 6 consecutive days.
33645130	14	114	theme	lung	2069:2072	arg1	tissue					2074:2079	lung tissue	2069:2079	lung tissue	2069:2079	AP had a good anti-influenza virus activity in vitro, and could protect mice with kidney Yang deficiency syndrome by reducing the viral load in lung tissue, decreasing inflammation damage in lung tissue, and regulating the expression of inflammatory cytokines.
33645130	6	115	theme	Real-time	939:947	arg1	PCR					949:951	Real-time PCR	939:951	Real-time PCR	939:951	Real-time PCR was used to detect the relative expression of M gene of H1 N1 influenza virus and cytokines in lung tissue.
33645130	14	116	theme	good	1887:1890	arg1	activity					1913:1920	a good anti-influenza virus activity	1885:1920	a good anti-influenza virus activity	1885:1920	AP had a good anti-influenza virus activity in vitro, and could protect mice with kidney Yang deficiency syndrome by reducing the viral load in lung tissue, decreasing inflammation damage in lung tissue, and regulating the expression of inflammatory cytokines.
33599213	4	0	theme	Mature	736:741	arg1	control					806:812	a positive control	795:812	a positive control	795:812	Mature VSMCs cultured in both types of medium were used as a positive control.
33599213	4	0	theme	Mature	736:741	arg1	VSMCs					743:747	Mature VSMCs	736:747	Mature VSMCs cultured in both types of medium	736:780	Mature VSMCs cultured in both types of medium were used as a positive control.
33599213	7	1	theme	heavy	1312:1316	arg1	chain					1318:1322	smooth muscle myosin heavy chain	1291:1322	smooth muscle myosin heavy chain	1291:1322	The differentiation medium supported the expression of specific early, mid-term and/or late markers of differentiation (i.e. type I collagen, αSMA, calponin, smoothelin, and smooth muscle myosin heavy chain) in ADSCs on all tested samples.
33599213	6	2	theme	ADSCs	1032:1036	arg1	growth					1022:1027	growth	1022:1027	growth	1022:1027	We observed similar adhesion and growth of ADSCs on all PLLA samples when they were cultured in the non-differentiation medium.
33599213	6	2	theme	ADSCs	1032:1036	arg1	adhesion					1009:1016	similar adhesion	1001:1016	similar adhesion	1001:1016	We observed similar adhesion and growth of ADSCs on all PLLA samples when they were cultured in the non-differentiation medium.
33599213	1	3	theme	smooth	234:239	arg1	VSMCs					255:259	VSMCs	255:259	VSMCs	255:259	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	1	3	theme	smooth	234:239	arg1	cells					248:252	vascular smooth muscle cells	225:252	vascular smooth muscle cells (VSMCs)	225:260	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	10	4	from	VSMCs	1710:1714	arg1	medium					1739:1744	the differentiation medium	1719:1744	the differentiation medium	1719:1744	Culturing VSMCs in the differentiation medium inhibited their metabolic activity and reduced the cell numbers.
33599213	7	5	theme	muscle	1298:1303	arg1	chain					1318:1322	smooth muscle myosin heavy chain	1291:1322	smooth muscle myosin heavy chain	1291:1322	The differentiation medium supported the expression of specific early, mid-term and/or late markers of differentiation (i.e. type I collagen, αSMA, calponin, smoothelin, and smooth muscle myosin heavy chain) in ADSCs on all tested samples.
33599213	6	6	theme	non-differentiation	1089:1107	arg1	medium					1109:1114	the non-differentiation medium	1085:1114	the non-differentiation medium	1085:1114	We observed similar adhesion and growth of ADSCs on all PLLA samples when they were cultured in the non-differentiation medium.
33599213	1	7	theme	polyethylene	378:389	arg1	glycol					391:396	polyethylene glycol	378:396	polyethylene glycol (PEG)	378:402	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	1	7	theme	polyethylene	378:389	arg1	PEG					399:401	PEG	399:401	PEG	399:401	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	8	8	theme	VSMCs	1543:1547	arg1	cultures					1531:1538	the cultures	1527:1538	the cultures of VSMCs	1527:1547	Moreover, ADSCs cultured in the differentiation medium revealed significant differences in cell growth among the samples that were similar to the differences observed in the cultures of VSMCs.
33599213	2	9	theme	ascorbic	611:618	arg1	medium					647:652	i.e. differentiation medium	626:652	i.e. differentiation medium	626:652	On these materials, the ADSCs were biochemically differentiated towards VSMCs by a medium supplemented with TGFβ1, BMP4 and ascorbic acid (i.e. differentiation medium).
33599213	2	9	theme	ascorbic	611:618	arg1	acid					620:623	ascorbic acid	611:623	ascorbic acid (i.e. differentiation medium)	611:653	On these materials, the ADSCs were biochemically differentiated towards VSMCs by a medium supplemented with TGFβ1, BMP4 and ascorbic acid (i.e. differentiation medium).
33599213	12	10	theme	tested	1946:1951	arg1	foils					1958:1962	the tested PLLA foils	1942:1962	the tested PLLA foils	1942:1962	The behaviour of ADSCs and VSMCs on the tested PLLA foils differed according to the specific cell type and culture conditions.
33599213	10	11	theme	cell	1797:1800	arg1	numbers					1802:1808	the cell numbers	1793:1808	the cell numbers	1793:1808	Culturing VSMCs in the differentiation medium inhibited their metabolic activity and reduced the cell numbers.
33599213	11	12	theme	plasma-treated	1863:1876	arg1	PLLA					1878:1881	plasma-treated PLLA	1863:1881	plasma-treated PLLA	1863:1881	Both cell types formed the most stable monolayer on plasma-treated PLLA and on the PS control.
33599213	13	13	theme	tissue	2143:2148	arg1	purposes					2162:2169	vascular tissue engineering purposes	2134:2169	vascular tissue engineering purposes	2134:2169	The suitable biocompatibility of both cell types on the tested PLLA foils seems to be favourable for vascular tissue engineering purposes.
33599213	7	14	theme	I	1247:1247	arg1	collagen					1249:1256	type I collagen	1242:1256	type I collagen	1242:1256	The differentiation medium supported the expression of specific early, mid-term and/or late markers of differentiation (i.e. type I collagen, αSMA, calponin, smoothelin, and smooth muscle myosin heavy chain) in ADSCs on all tested samples.
33599213	0	15	from	cells	84:88	arg1	foils					118:122	modified poly(L-lactide) foils	93:122	modified poly(L-lactide) foils	93:122	Differentiation of adipose tissue-derived stem cells towards vascular smooth muscle cells on modified poly(L-lactide) foils.
33599213	2	16	theme	differentiation	631:645	arg1	medium					647:652	i.e. differentiation medium	626:652	i.e. differentiation medium	626:652	On these materials, the ADSCs were biochemically differentiated towards VSMCs by a medium supplemented with TGFβ1, BMP4 and ascorbic acid (i.e. differentiation medium).
33599213	2	16	theme	differentiation	631:645	arg1	acid					620:623	ascorbic acid	611:623	ascorbic acid (i.e. differentiation medium)	611:653	On these materials, the ADSCs were biochemically differentiated towards VSMCs by a medium supplemented with TGFβ1, BMP4 and ascorbic acid (i.e. differentiation medium).
33599213	1	17	theme	tissue-derived	187:200	arg1	ADSCs					214:218	ADSCs	214:218	ADSCs	214:218	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	1	17	theme	tissue-derived	187:200	arg1	cells					207:211	adipose tissue-derived stem cells	179:211	adipose tissue-derived stem cells (ADSCs)	179:219	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	7	18	theme	differentiation	1220:1234	arg1	markers					1209:1215	specific early, mid-term and/or late markers	1172:1215	specific early, mid-term and/or late markers of differentiation	1172:1234	The differentiation medium supported the expression of specific early, mid-term and/or late markers of differentiation (i.e. type I collagen, αSMA, calponin, smoothelin, and smooth muscle myosin heavy chain) in ADSCs on all tested samples.
33599213	11	19	theme	PS	1894:1895	arg1	control					1897:1903	the PS control	1890:1903	the PS control	1890:1903	Both cell types formed the most stable monolayer on plasma-treated PLLA and on the PS control.
33599213	1	20	from	behaviour	166:174	arg1	foils					307:311	variously modified poly(L-lactide) (PLLA) foils	265:311	variously modified poly(L-lactide) (PLLA) foils	265:311	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	3	21	theme	negative	718:725	arg1	ADSCs					656:660	ADSCs	656:660	ADSCs cultured in a non-differentiation medium	656:701	ADSCs cultured in a non-differentiation medium were used as a negative control.
33599213	3	21	theme	negative	718:725	arg1	control					727:733	a negative control	716:733	a negative control	716:733	ADSCs cultured in a non-differentiation medium were used as a negative control.
33599213	9	22	theme	round	1554:1558	arg1	morphology					1560:1569	The round morphology	1550:1569	The round morphology of the VSMCs	1550:1582	The round morphology of the VSMCs indicated worse adhesion to pristine PLLA, and this sample was also characterized by the lowest cell proliferation.
33599213	7	23	theme	markers	1209:1215	arg1	expression					1158:1167	the expression	1154:1167	the expression of specific early, mid-term and/or late markers of differentiation	1154:1234	The differentiation medium supported the expression of specific early, mid-term and/or late markers of differentiation (i.e. type I collagen, αSMA, calponin, smoothelin, and smooth muscle myosin heavy chain) in ADSCs on all tested samples.
33599213	1	24	theme	plasma-treated	339:352	arg1	PLLA					333:336	pristine PLLA	324:336	pristine PLLA	324:336	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	1	24	theme	plasma-treated	339:352	arg1	PLLA					354:357	plasma-treated PLLA	339:357	plasma-treated PLLA	339:357	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	11	25	theme	cell	1816:1819	arg1	types					1821:1825	Both cell types	1811:1825	Both cell types	1811:1825	Both cell types formed the most stable monolayer on plasma-treated PLLA and on the PS control.
33599213	13	26	theme	PLLA	2096:2099	arg1	foils					2101:2105	the tested PLLA foils	2085:2105	the tested PLLA foils	2085:2105	The suitable biocompatibility of both cell types on the tested PLLA foils seems to be favourable for vascular tissue engineering purposes.
33599213	0	27	theme	smooth	70:75	arg1	cells					84:88	vascular smooth muscle cells	61:88	vascular smooth muscle cells on modified poly(L-lactide) foils	61:122	Differentiation of adipose tissue-derived stem cells towards vascular smooth muscle cells on modified poly(L-lactide) foils.
33599213	1	28	theme	poly	284:287	arg1	foils					307:311	variously modified poly(L-lactide) (PLLA) foils	265:311	variously modified poly(L-lactide) (PLLA) foils	265:311	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	7	29	dep	collagen	1249:1256	arg1	i.e.					1237:1240	i.e.	1237:1240	i.e.	1237:1240	The differentiation medium supported the expression of specific early, mid-term and/or late markers of differentiation (i.e. type I collagen, αSMA, calponin, smoothelin, and smooth muscle myosin heavy chain) in ADSCs on all tested samples.
33599213	9	30	theme	VSMCs	1578:1582	arg1	morphology					1560:1569	The round morphology	1550:1569	The round morphology of the VSMCs	1550:1582	The round morphology of the VSMCs indicated worse adhesion to pristine PLLA, and this sample was also characterized by the lowest cell proliferation.
33599213	1	31	theme	research	140:147	arg1	aim					129:131	The aim	125:131	The aim of our research	125:147	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	9	32	theme	worse	1594:1598	arg1	adhesion					1600:1607	worse adhesion	1594:1607	worse adhesion to pristine PLLA	1594:1624	The round morphology of the VSMCs indicated worse adhesion to pristine PLLA, and this sample was also characterized by the lowest cell proliferation.
33599213	3	33	theme	non-differentiation	676:694	arg1	medium					696:701	a non-differentiation medium	674:701	a non-differentiation medium	674:701	ADSCs cultured in a non-differentiation medium were used as a negative control.
33599213	0	34	theme	modified	93:100	arg1	foils					118:122	modified poly(L-lactide) foils	93:122	modified poly(L-lactide) foils	93:122	Differentiation of adipose tissue-derived stem cells towards vascular smooth muscle cells on modified poly(L-lactide) foils.
33599213	1	35	theme	polystyrene	461:471	arg1	control					478:484	the tissue culture polystyrene (PS) control	442:484	the tissue culture polystyrene (PS) control	442:484	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	7	36	theme	mid-term	1188:1195	arg1	markers					1209:1215	specific early, mid-term and/or late markers	1172:1215	specific early, mid-term and/or late markers of differentiation	1172:1234	The differentiation medium supported the expression of specific early, mid-term and/or late markers of differentiation (i.e. type I collagen, αSMA, calponin, smoothelin, and smooth muscle myosin heavy chain) in ADSCs on all tested samples.
33599213	1	37	from	foils	307:311	arg1	PLLA					405:408	PLLA	405:408	PLLA	405:408	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	1	37	from	foils	307:311	arg1	PLLA					360:363	PLLA	360:363	PLLA grafted with polyethylene glycol (PEG)	360:402	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	1	37	from	foils	307:311	arg1	PLLA					333:336	pristine PLLA	324:336	pristine PLLA	324:336	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	1	37	from	foils	307:311	arg1	PLLA					354:357	plasma-treated PLLA	339:357	plasma-treated PLLA	339:357	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	1	38	theme	muscle	241:246	arg1	VSMCs					255:259	VSMCs	255:259	VSMCs	255:259	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	1	38	theme	muscle	241:246	arg1	cells					248:252	vascular smooth muscle cells	225:252	vascular smooth muscle cells (VSMCs)	225:260	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	7	39	theme	early	1181:1185	arg1	markers					1209:1215	specific early, mid-term and/or late markers	1172:1215	specific early, mid-term and/or late markers of differentiation	1172:1234	The differentiation medium supported the expression of specific early, mid-term and/or late markers of differentiation (i.e. type I collagen, αSMA, calponin, smoothelin, and smooth muscle myosin heavy chain) in ADSCs on all tested samples.
33599213	1	40	theme	culture	453:459	arg1	control					478:484	the tissue culture polystyrene (PS) control	442:484	the tissue culture polystyrene (PS) control	442:484	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	0	41	theme	adipose	19:25	arg1	cells					47:51	adipose tissue-derived stem cells	19:51	adipose tissue-derived stem cells	19:51	Differentiation of adipose tissue-derived stem cells towards vascular smooth muscle cells on modified poly(L-lactide) foils.
33599213	13	42	theme	suitable	2037:2044	arg1	favourable					2119:2128	favourable	2119:2128	favourable	2119:2128	The suitable biocompatibility of both cell types on the tested PLLA foils seems to be favourable for vascular tissue engineering purposes.
33599213	13	42	theme	suitable	2037:2044	arg1	biocompatibility					2046:2061	The suitable biocompatibility	2033:2061	The suitable biocompatibility of both cell types on the tested PLLA foils	2033:2105	The suitable biocompatibility of both cell types on the tested PLLA foils seems to be favourable for vascular tissue engineering purposes.
33599213	0	43	theme	stem	42:45	arg1	cells					47:51	adipose tissue-derived stem cells	19:51	adipose tissue-derived stem cells	19:51	Differentiation of adipose tissue-derived stem cells towards vascular smooth muscle cells on modified poly(L-lactide) foils.
33599213	7	44	theme	tested	1341:1346	arg1	samples					1348:1354	all tested samples	1337:1354	all tested samples	1337:1354	The differentiation medium supported the expression of specific early, mid-term and/or late markers of differentiation (i.e. type I collagen, αSMA, calponin, smoothelin, and smooth muscle myosin heavy chain) in ADSCs on all tested samples.
33599213	5	45	theme	differences	870:880	arg1	impact					819:824	The impact	815:824	The impact of the variously modified PLLA foils and/or differences in the composition of the medium	815:913	The impact of the variously modified PLLA foils and/or differences in the composition of the medium were studied with reference to cell adhesion, growth and differentiation.
33599213	12	46	theme	cell	1999:2002	arg1	type					2004:2007	the specific cell type and culture conditions	1986:2030	type	2004:2007	The behaviour of ADSCs and VSMCs on the tested PLLA foils differed according to the specific cell type and culture conditions.
33599213	10	47	theme	Culturing	1700:1708	arg1	VSMCs					1710:1714	Culturing VSMCs	1700:1714	Culturing VSMCs in the differentiation medium	1700:1744	Culturing VSMCs in the differentiation medium inhibited their metabolic activity and reduced the cell numbers.
33599213	1	48	theme	stem	202:205	arg1	ADSCs					214:218	ADSCs	214:218	ADSCs	214:218	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	1	48	theme	stem	202:205	arg1	cells					207:211	adipose tissue-derived stem cells	179:211	adipose tissue-derived stem cells (ADSCs)	179:219	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	8	49	located	observed	1515:1522	arg2	differences					1503:1513	the differences	1499:1513	the differences observed in the cultures of VSMCs	1499:1547	Moreover, ADSCs cultured in the differentiation medium revealed significant differences in cell growth among the samples that were similar to the differences observed in the cultures of VSMCs.
33599213	8	49	located	observed	1515:1522	arg1	cultures					1531:1538	the cultures	1527:1538	the cultures of VSMCs	1527:1547	Moreover, ADSCs cultured in the differentiation medium revealed significant differences in cell growth among the samples that were similar to the differences observed in the cultures of VSMCs.
33599213	5	50	theme	modified	843:850	arg1	foils					857:861	the variously modified PLLA foils	829:861	the variously modified PLLA foils	829:861	The impact of the variously modified PLLA foils and/or differences in the composition of the medium were studied with reference to cell adhesion, growth and differentiation.
33599213	9	51	theme	lowest	1673:1678	arg1	proliferation					1685:1697	the lowest cell proliferation	1669:1697	the lowest cell proliferation	1669:1697	The round morphology of the VSMCs indicated worse adhesion to pristine PLLA, and this sample was also characterized by the lowest cell proliferation.
33599213	8	52	theme	significant	1421:1431	arg1	differences					1433:1443	significant differences	1421:1443	significant differences in cell growth among the samples that were similar to the differences observed in the cultures of VSMCs	1421:1547	Moreover, ADSCs cultured in the differentiation medium revealed significant differences in cell growth among the samples that were similar to the differences observed in the cultures of VSMCs.
33599213	7	53	from	ADSCs	1328:1332	arg1	samples					1348:1354	all tested samples	1337:1354	all tested samples	1337:1354	The differentiation medium supported the expression of specific early, mid-term and/or late markers of differentiation (i.e. type I collagen, αSMA, calponin, smoothelin, and smooth muscle myosin heavy chain) in ADSCs on all tested samples.
33599213	5	54	theme	foils	857:861	arg1	impact					819:824	The impact	815:824	The impact of the variously modified PLLA foils and/or differences in the composition of the medium	815:913	The impact of the variously modified PLLA foils and/or differences in the composition of the medium were studied with reference to cell adhesion, growth and differentiation.
33599213	10	55	theme	differentiation	1723:1737	arg1	medium					1739:1744	the differentiation medium	1719:1744	the differentiation medium	1719:1744	Culturing VSMCs in the differentiation medium inhibited their metabolic activity and reduced the cell numbers.
33599213	7	56	theme	myosin	1305:1310	arg1	chain					1318:1322	smooth muscle myosin heavy chain	1291:1322	smooth muscle myosin heavy chain	1291:1322	The differentiation medium supported the expression of specific early, mid-term and/or late markers of differentiation (i.e. type I collagen, αSMA, calponin, smoothelin, and smooth muscle myosin heavy chain) in ADSCs on all tested samples.
33599213	0	57	link	tissue-derived	27:40	arg1	cells					47:51	adipose tissue-derived stem cells	19:51	adipose tissue-derived stem cells	19:51	Differentiation of adipose tissue-derived stem cells towards vascular smooth muscle cells on modified poly(L-lactide) foils.
33599213	5	58	from	impact	819:824	arg1	composition					889:899	the composition	885:899	the composition of the medium	885:913	The impact of the variously modified PLLA foils and/or differences in the composition of the medium were studied with reference to cell adhesion, growth and differentiation.
33599213	1	59	theme	vascular	225:232	arg1	VSMCs					255:259	VSMCs	255:259	VSMCs	255:259	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	1	59	theme	vascular	225:232	arg1	cells					248:252	vascular smooth muscle cells	225:252	vascular smooth muscle cells (VSMCs)	225:260	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	7	60	theme	smooth	1291:1296	arg1	chain					1318:1322	smooth muscle myosin heavy chain	1291:1322	smooth muscle myosin heavy chain	1291:1322	The differentiation medium supported the expression of specific early, mid-term and/or late markers of differentiation (i.e. type I collagen, αSMA, calponin, smoothelin, and smooth muscle myosin heavy chain) in ADSCs on all tested samples.
33599213	2	61	theme	i.e.	626:629	arg1	medium					647:652	i.e. differentiation medium	626:652	i.e. differentiation medium	626:652	On these materials, the ADSCs were biochemically differentiated towards VSMCs by a medium supplemented with TGFβ1, BMP4 and ascorbic acid (i.e. differentiation medium).
33599213	2	61	theme	i.e.	626:629	arg1	acid					620:623	ascorbic acid	611:623	ascorbic acid (i.e. differentiation medium)	611:653	On these materials, the ADSCs were biochemically differentiated towards VSMCs by a medium supplemented with TGFβ1, BMP4 and ascorbic acid (i.e. differentiation medium).
33599213	10	62	theme	metabolic	1762:1770	arg1	activity					1772:1779	their metabolic activity	1756:1779	their metabolic activity	1756:1779	Culturing VSMCs in the differentiation medium inhibited their metabolic activity and reduced the cell numbers.
33599213	12	63	theme	VSMCs	1933:1937	arg1	behaviour					1910:1918	The behaviour	1906:1918	The behaviour of ADSCs and VSMCs on the tested PLLA foils	1906:1962	The behaviour of ADSCs and VSMCs on the tested PLLA foils differed according to the specific cell type and culture conditions.
33599213	13	64	theme	vascular	2134:2141	arg1	purposes					2162:2169	vascular tissue engineering purposes	2134:2169	vascular tissue engineering purposes	2134:2169	The suitable biocompatibility of both cell types on the tested PLLA foils seems to be favourable for vascular tissue engineering purposes.
33599213	6	65	theme	similar	1001:1007	arg1	adhesion					1009:1016	similar adhesion	1001:1016	similar adhesion	1001:1016	We observed similar adhesion and growth of ADSCs on all PLLA samples when they were cultured in the non-differentiation medium.
33599213	11	66	theme	stable	1843:1848	arg1	monolayer					1850:1858	the most stable monolayer	1834:1858	the most stable monolayer on plasma-treated PLLA and on the PS control	1834:1903	Both cell types formed the most stable monolayer on plasma-treated PLLA and on the PS control.
33599213	4	67	theme	medium	775:780	arg1	types					766:770	both types	761:770	both types of medium	761:780	Mature VSMCs cultured in both types of medium were used as a positive control.
33599213	13	68	theme	types	2076:2080	arg1	favourable					2119:2128	favourable	2119:2128	favourable	2119:2128	The suitable biocompatibility of both cell types on the tested PLLA foils seems to be favourable for vascular tissue engineering purposes.
33599213	13	68	theme	types	2076:2080	arg1	biocompatibility					2046:2061	The suitable biocompatibility	2033:2061	The suitable biocompatibility of both cell types on the tested PLLA foils	2033:2105	The suitable biocompatibility of both cell types on the tested PLLA foils seems to be favourable for vascular tissue engineering purposes.
33599213	8	69	from	differences	1433:1443	arg1	growth					1453:1458	cell growth	1448:1458	cell growth	1448:1458	Moreover, ADSCs cultured in the differentiation medium revealed significant differences in cell growth among the samples that were similar to the differences observed in the cultures of VSMCs.
33599213	5	70	from	differences	870:880	arg1	composition					889:899	the composition	885:899	the composition of the medium	885:913	The impact of the variously modified PLLA foils and/or differences in the composition of the medium were studied with reference to cell adhesion, growth and differentiation.
33599213	1	71	theme	adipose	179:185	arg1	ADSCs					214:218	ADSCs	214:218	ADSCs	214:218	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	1	71	theme	adipose	179:185	arg1	cells					207:211	adipose tissue-derived stem cells	179:211	adipose tissue-derived stem cells (ADSCs)	179:219	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	7	72	theme	type	1242:1245	arg1	collagen					1249:1256	type I collagen	1242:1256	type I collagen	1242:1256	The differentiation medium supported the expression of specific early, mid-term and/or late markers of differentiation (i.e. type I collagen, αSMA, calponin, smoothelin, and smooth muscle myosin heavy chain) in ADSCs on all tested samples.
33599213	12	73	theme	ADSCs	1923:1927	arg1	behaviour					1910:1918	The behaviour	1906:1918	The behaviour of ADSCs and VSMCs on the tested PLLA foils	1906:1962	The behaviour of ADSCs and VSMCs on the tested PLLA foils differed according to the specific cell type and culture conditions.
33599213	4	74	theme	positive	797:804	arg1	control					806:812	a positive control	795:812	a positive control	795:812	Mature VSMCs cultured in both types of medium were used as a positive control.
33599213	4	74	theme	positive	797:804	arg1	VSMCs					743:747	Mature VSMCs	736:747	Mature VSMCs cultured in both types of medium	736:780	Mature VSMCs cultured in both types of medium were used as a positive control.
33599213	1	75	theme	pristine	324:331	arg1	PLLA					405:408	PLLA	405:408	PLLA	405:408	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	1	75	theme	pristine	324:331	arg1	PLLA					360:363	PLLA	360:363	PLLA grafted with polyethylene glycol (PEG)	360:402	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	1	75	theme	pristine	324:331	arg1	PLLA					333:336	pristine PLLA	324:336	pristine PLLA	324:336	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	1	75	theme	pristine	324:331	arg1	PLLA					354:357	plasma-treated PLLA	339:357	plasma-treated PLLA	339:357	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	1	76	theme	PS	474:475	arg1	control					478:484	the tissue culture polystyrene (PS) control	442:484	the tissue culture polystyrene (PS) control	442:484	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	13	77	theme	tested	2089:2094	arg1	foils					2101:2105	the tested PLLA foils	2085:2105	the tested PLLA foils	2085:2105	The suitable biocompatibility of both cell types on the tested PLLA foils seems to be favourable for vascular tissue engineering purposes.
33599213	6	78	theme	PLLA	1045:1048	arg1	samples					1050:1056	all PLLA samples	1041:1056	all PLLA samples	1041:1056	We observed similar adhesion and growth of ADSCs on all PLLA samples when they were cultured in the non-differentiation medium.
33599213	0	79	theme	muscle	77:82	arg1	cells					84:88	vascular smooth muscle cells	61:88	vascular smooth muscle cells on modified poly(L-lactide) foils	61:122	Differentiation of adipose tissue-derived stem cells towards vascular smooth muscle cells on modified poly(L-lactide) foils.
33599213	5	80	theme	medium	908:913	arg1	composition					889:899	the composition	885:899	the composition of the medium	885:913	The impact of the variously modified PLLA foils and/or differences in the composition of the medium were studied with reference to cell adhesion, growth and differentiation.
33599213	7	81	theme	late	1204:1207	arg1	markers					1209:1215	specific early, mid-term and/or late markers	1172:1215	specific early, mid-term and/or late markers of differentiation	1172:1234	The differentiation medium supported the expression of specific early, mid-term and/or late markers of differentiation (i.e. type I collagen, αSMA, calponin, smoothelin, and smooth muscle myosin heavy chain) in ADSCs on all tested samples.
33599213	4	82	used	used	787:790	arg2	VSMCs					743:747	Mature VSMCs	736:747	Mature VSMCs cultured in both types of medium	736:780	Mature VSMCs cultured in both types of medium were used as a positive control.
33599213	4	82	used	used	787:790	arg2	control					806:812	a positive control	795:812	a positive control	795:812	Mature VSMCs cultured in both types of medium were used as a positive control.
33599213	5	83	from	composition	889:899	arg1	impact					819:824	The impact	815:824	The impact of the variously modified PLLA foils and/or differences in the composition of the medium	815:913	The impact of the variously modified PLLA foils and/or differences in the composition of the medium were studied with reference to cell adhesion, growth and differentiation.
33599213	13	84	theme	cell	2071:2074	arg1	types					2076:2080	both cell types	2066:2080	both cell types	2066:2080	The suitable biocompatibility of both cell types on the tested PLLA foils seems to be favourable for vascular tissue engineering purposes.
33599213	1	85	link	tissue-derived	187:200	arg1	ADSCs					214:218	ADSCs	214:218	ADSCs	214:218	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	1	85	link	tissue-derived	187:200	arg1	cells					207:211	adipose tissue-derived stem cells	179:211	adipose tissue-derived stem cells (ADSCs)	179:219	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	0	86	theme	poly	102:105	arg1	foils					118:122	modified poly(L-lactide) foils	93:122	modified poly(L-lactide) foils	93:122	Differentiation of adipose tissue-derived stem cells towards vascular smooth muscle cells on modified poly(L-lactide) foils.
33599213	6	87	from	growth	1022:1027	arg1	samples					1050:1056	all PLLA samples	1041:1056	all PLLA samples	1041:1056	We observed similar adhesion and growth of ADSCs on all PLLA samples when they were cultured in the non-differentiation medium.
33599213	5	88	theme	cell	946:949	arg1	adhesion					951:958	cell adhesion	946:958	cell adhesion	946:958	The impact of the variously modified PLLA foils and/or differences in the composition of the medium were studied with reference to cell adhesion, growth and differentiation.
33599213	9	89	theme	pristine	1612:1619	arg1	PLLA					1621:1624	pristine PLLA	1612:1624	pristine PLLA	1612:1624	The round morphology of the VSMCs indicated worse adhesion to pristine PLLA, and this sample was also characterized by the lowest cell proliferation.
33599213	3	90	used	used	708:711	arg2	ADSCs					656:660	ADSCs	656:660	ADSCs cultured in a non-differentiation medium	656:701	ADSCs cultured in a non-differentiation medium were used as a negative control.
33599213	3	90	used	used	708:711	arg2	control					727:733	a negative control	716:733	a negative control	716:733	ADSCs cultured in a non-differentiation medium were used as a negative control.
33599213	13	91	from	biocompatibility	2046:2061	arg1	foils					2101:2105	the tested PLLA foils	2085:2105	the tested PLLA foils	2085:2105	The suitable biocompatibility of both cell types on the tested PLLA foils seems to be favourable for vascular tissue engineering purposes.
33599213	7	92	theme	specific	1172:1179	arg1	markers					1209:1215	specific early, mid-term and/or late markers	1172:1215	specific early, mid-term and/or late markers of differentiation	1172:1234	The differentiation medium supported the expression of specific early, mid-term and/or late markers of differentiation (i.e. type I collagen, αSMA, calponin, smoothelin, and smooth muscle myosin heavy chain) in ADSCs on all tested samples.
33599213	1	93	theme	tissue	446:451	arg1	control					478:484	the tissue culture polystyrene (PS) control	442:484	the tissue culture polystyrene (PS) control	442:484	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	0	94	theme	tissue-derived	27:40	arg1	cells					47:51	adipose tissue-derived stem cells	19:51	adipose tissue-derived stem cells	19:51	Differentiation of adipose tissue-derived stem cells towards vascular smooth muscle cells on modified poly(L-lactide) foils.
33599213	1	95	theme	cells	248:252	arg1	behaviour					166:174	the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control	162:484	the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control	162:484	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	12	96	from	behaviour	1910:1918	arg1	foils					1958:1962	the tested PLLA foils	1942:1962	the tested PLLA foils	1942:1962	The behaviour of ADSCs and VSMCs on the tested PLLA foils differed according to the specific cell type and culture conditions.
33599213	12	97	theme	culture	2013:2019	arg1	conditions					2021:2030	the specific cell type and culture conditions	1986:2030	conditions	2021:2030	The behaviour of ADSCs and VSMCs on the tested PLLA foils differed according to the specific cell type and culture conditions.
33599213	6	98	from	adhesion	1009:1016	arg1	samples					1050:1056	all PLLA samples	1041:1056	all PLLA samples	1041:1056	We observed similar adhesion and growth of ADSCs on all PLLA samples when they were cultured in the non-differentiation medium.
33599213	0	99	theme	cells	47:51	arg1	Differentiation					0:14	Differentiation	0:14	Differentiation of adipose tissue-derived stem cells towards vascular smooth muscle cells on modified poly(L-lactide) foils.	0:123	Differentiation of adipose tissue-derived stem cells towards vascular smooth muscle cells on modified poly(L-lactide) foils.
33599213	8	100	theme	differentiation	1389:1403	arg1	medium					1405:1410	the differentiation medium	1385:1410	the differentiation medium	1385:1410	Moreover, ADSCs cultured in the differentiation medium revealed significant differences in cell growth among the samples that were similar to the differences observed in the cultures of VSMCs.
33599213	0	101	theme	vascular	61:68	arg1	cells					84:88	vascular smooth muscle cells	61:88	vascular smooth muscle cells on modified poly(L-lactide) foils	61:122	Differentiation of adipose tissue-derived stem cells towards vascular smooth muscle cells on modified poly(L-lactide) foils.
33599213	7	102	theme	differentiation	1121:1135	arg1	medium					1137:1142	The differentiation medium	1117:1142	The differentiation medium	1117:1142	The differentiation medium supported the expression of specific early, mid-term and/or late markers of differentiation (i.e. type I collagen, αSMA, calponin, smoothelin, and smooth muscle myosin heavy chain) in ADSCs on all tested samples.
33599213	12	103	theme	specific	1990:1997	arg1	type					2004:2007	the specific cell type and culture conditions	1986:2030	type	2004:2007	The behaviour of ADSCs and VSMCs on the tested PLLA foils differed according to the specific cell type and culture conditions.
33599213	1	104	theme	modified	275:282	arg1	L-lactide					289:297	L-lactide	289:297	L-lactide	289:297	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	1	104	theme	modified	275:282	arg1	PLLA					301:304	PLLA	301:304	PLLA	301:304	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	1	104	theme	modified	275:282	arg1	poly					284:287	variously modified poly	265:287	variously modified poly(L-lactide) (PLLA) foils	265:311	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	13	105	theme	engineering	2150:2160	arg1	purposes					2162:2169	vascular tissue engineering purposes	2134:2169	vascular tissue engineering purposes	2134:2169	The suitable biocompatibility of both cell types on the tested PLLA foils seems to be favourable for vascular tissue engineering purposes.
33599213	11	106	from	monolayer	1850:1858	arg1	control					1897:1903	the PS control	1890:1903	the PS control	1890:1903	Both cell types formed the most stable monolayer on plasma-treated PLLA and on the PS control.
33599213	11	106	from	monolayer	1850:1858	arg1	PLLA					1878:1881	plasma-treated PLLA	1863:1881	plasma-treated PLLA	1863:1881	Both cell types formed the most stable monolayer on plasma-treated PLLA and on the PS control.
33599213	12	107	theme	PLLA	1953:1956	arg1	foils					1958:1962	the tested PLLA foils	1942:1962	the tested PLLA foils	1942:1962	The behaviour of ADSCs and VSMCs on the tested PLLA foils differed according to the specific cell type and culture conditions.
33599213	1	108	theme	cells	207:211	arg1	behaviour					166:174	the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control	162:484	the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control	162:484	The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control.
33599213	5	109	theme	PLLA	852:855	arg1	foils					857:861	the variously modified PLLA foils	829:861	the variously modified PLLA foils	829:861	The impact of the variously modified PLLA foils and/or differences in the composition of the medium were studied with reference to cell adhesion, growth and differentiation.
33599213	9	110	theme	cell	1680:1683	arg1	proliferation					1685:1697	the lowest cell proliferation	1669:1697	the lowest cell proliferation	1669:1697	The round morphology of the VSMCs indicated worse adhesion to pristine PLLA, and this sample was also characterized by the lowest cell proliferation.
33599213	8	111	theme	cell	1448:1451	arg1	growth					1453:1458	cell growth	1448:1458	cell growth	1448:1458	Moreover, ADSCs cultured in the differentiation medium revealed significant differences in cell growth among the samples that were similar to the differences observed in the cultures of VSMCs.
34907778	6	0	theme	CMA	1170:1172	arg1	dressing					1183:1190	the Rh-EGF-loaded CMA hydrogel dressing	1152:1190	the Rh-EGF-loaded CMA hydrogel dressing	1152:1190	The investigation in vivo showed that the Rh-EGF-loaded CMA hydrogel dressing significantly enhanced wound healing and reduced inflammatory responses by upregulating the transforming growth factor-beta, vascular endothelial growth factor, and cluster of differentiation 31 (CD31).
34907778	2	1	theme	drug-loaded	411:421	arg1	dressing					438:445	a stimuli-responsive drug-loaded hydrogel wound dressing	390:445	a stimuli-responsive drug-loaded hydrogel wound dressing for sustained, controlled release of the drug and accelerating wound healing	390:522	Hence, we developed a stimuli-responsive drug-loaded hydrogel wound dressing for sustained, controlled release of the drug and accelerating wound healing.
34907778	6	2	theme	hydrogel	1174:1181	arg1	dressing					1183:1190	the Rh-EGF-loaded CMA hydrogel dressing	1152:1190	the Rh-EGF-loaded CMA hydrogel dressing	1152:1190	The investigation in vivo showed that the Rh-EGF-loaded CMA hydrogel dressing significantly enhanced wound healing and reduced inflammatory responses by upregulating the transforming growth factor-beta, vascular endothelial growth factor, and cluster of differentiation 31 (CD31).
34907778	4	3	theme	release	968:974	arg1	media					976:980	various release media	960:980	various release media	960:980	Experimental results indicated that these hydrogel composites showed pH and temperature stimuli-responsive behaviors and released the encapsulated drug sustainedly in various release media.
34907778	5	4	theme	hydrogel	997:1004	arg1	dressings					1006:1014	the hydrogel dressings	993:1014	the hydrogel dressings	993:1014	Moreover, the hydrogel dressings exhibited a porous network structure, stable physical properties, and excellent biocompatibility.
34907778	2	5	theme	stimuli-responsive	392:409	arg1	dressing					438:445	a stimuli-responsive drug-loaded hydrogel wound dressing	390:445	a stimuli-responsive drug-loaded hydrogel wound dressing for sustained, controlled release of the drug and accelerating wound healing	390:522	Hence, we developed a stimuli-responsive drug-loaded hydrogel wound dressing for sustained, controlled release of the drug and accelerating wound healing.
34907778	1	6	theme	wound	313:317	arg1	healing					319:325	wound healing	313:325	wound healing	313:325	Stimuli-responsive hydrogels have drawn increasing research interest in regenerative medicine due to their tunable molecular structures and functional properties for both providing a suitable microenvironment for wound healing and to serve as a sustainable therapeutic.
34907778	3	7	from	loading	710:716	arg1	hydrogel					783:790	the CMA hydrogel	775:790	the CMA hydrogel	775:790	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	4	8	theme	stimuli-responsive	881:898	arg1	behaviors					900:908	stimuli-responsive behaviors	881:908	stimuli-responsive behaviors	881:908	Experimental results indicated that these hydrogel composites showed pH and temperature stimuli-responsive behaviors and released the encapsulated drug sustainedly in various release media.
34907778	6	9	theme	vascular	1317:1324	arg1	factor					1345:1350	vascular endothelial growth factor	1317:1350	vascular endothelial growth factor	1317:1350	The investigation in vivo showed that the Rh-EGF-loaded CMA hydrogel dressing significantly enhanced wound healing and reduced inflammatory responses by upregulating the transforming growth factor-beta, vascular endothelial growth factor, and cluster of differentiation 31 (CD31).
34907778	7	10	theme	potential	1467:1475	arg1	application					1477:1487	potential application	1467:1487	potential application	1467:1487	These results confirm that Rh-EGF-loaded CMA hydrogel dressings possess potential application in accelerating wound healing and tissue regeneration.
34907778	5	11	theme	physical	1061:1068	arg1	properties					1070:1079	stable physical properties	1054:1079	stable physical properties	1054:1079	Moreover, the hydrogel dressings exhibited a porous network structure, stable physical properties, and excellent biocompatibility.
34907778	4	12	dep	pH	862:863	arg1	behaviors					900:908	stimuli-responsive behaviors	881:908	stimuli-responsive behaviors	881:908	Experimental results indicated that these hydrogel composites showed pH and temperature stimuli-responsive behaviors and released the encapsulated drug sustainedly in various release media.
34907778	3	13	with	agarose	613:619	arg1	degrees					640:646	various degrees	632:646	various degrees	632:646	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	4	14	theme	hydrogel	835:842	arg1	composites					844:853	these hydrogel composites	829:853	these hydrogel composites	829:853	Experimental results indicated that these hydrogel composites showed pH and temperature stimuli-responsive behaviors and released the encapsulated drug sustainedly in various release media.
34907778	3	15	theme	stimuli-responsive	549:566	arg1	properties					568:577	stimuli-responsive properties	549:577	stimuli-responsive properties	549:577	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	7	16	theme	Rh-EGF-loaded	1422:1434	arg1	dressings					1449:1457	Rh-EGF-loaded CMA hydrogel dressings	1422:1457	Rh-EGF-loaded CMA hydrogel dressings	1422:1457	These results confirm that Rh-EGF-loaded CMA hydrogel dressings possess potential application in accelerating wound healing and tissue regeneration.
34907778	6	17	theme	wound	1215:1219	arg1	healing					1221:1227	wound healing	1215:1227	wound healing	1215:1227	The investigation in vivo showed that the Rh-EGF-loaded CMA hydrogel dressing significantly enhanced wound healing and reduced inflammatory responses by upregulating the transforming growth factor-beta, vascular endothelial growth factor, and cluster of differentiation 31 (CD31).
34907778	6	18	theme	endothelial	1326:1336	arg1	factor					1345:1350	vascular endothelial growth factor	1317:1350	vascular endothelial growth factor	1317:1350	The investigation in vivo showed that the Rh-EGF-loaded CMA hydrogel dressing significantly enhanced wound healing and reduced inflammatory responses by upregulating the transforming growth factor-beta, vascular endothelial growth factor, and cluster of differentiation 31 (CD31).
34907778	5	19	theme	porous	1028:1033	arg1	structure					1043:1051	a porous network structure	1026:1051	a porous network structure	1026:1051	Moreover, the hydrogel dressings exhibited a porous network structure, stable physical properties, and excellent biocompatibility.
34907778	1	20	theme	tunable	207:213	arg1	structures					225:234	tunable molecular structures	207:234	tunable molecular structures	207:234	Stimuli-responsive hydrogels have drawn increasing research interest in regenerative medicine due to their tunable molecular structures and functional properties for both providing a suitable microenvironment for wound healing and to serve as a sustainable therapeutic.
34907778	3	21	theme	CMA	779:781	arg1	hydrogel					783:790	the CMA hydrogel	775:790	the CMA hydrogel	775:790	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	4	22	theme	drug	940:943	arg1	sustainedly					945:955	the encapsulated drug sustainedly	923:955	the encapsulated drug sustainedly in various release media	923:980	Experimental results indicated that these hydrogel composites showed pH and temperature stimuli-responsive behaviors and released the encapsulated drug sustainedly in various release media.
34907778	6	23	theme	growth	1297:1302	arg1	factor-beta					1304:1314	the transforming growth factor-beta	1280:1314	the transforming growth factor-beta	1280:1314	The investigation in vivo showed that the Rh-EGF-loaded CMA hydrogel dressing significantly enhanced wound healing and reduced inflammatory responses by upregulating the transforming growth factor-beta, vascular endothelial growth factor, and cluster of differentiation 31 (CD31).
34907778	1	24	theme	Stimuli-responsive	100:117	arg1	hydrogels					119:127	Stimuli-responsive hydrogels	100:127	Stimuli-responsive hydrogels	100:127	Stimuli-responsive hydrogels have drawn increasing research interest in regenerative medicine due to their tunable molecular structures and functional properties for both providing a suitable microenvironment for wound healing and to serve as a sustainable therapeutic.
34907778	1	25	theme	molecular	215:223	arg1	structures					225:234	tunable molecular structures	207:234	tunable molecular structures	207:234	Stimuli-responsive hydrogels have drawn increasing research interest in regenerative medicine due to their tunable molecular structures and functional properties for both providing a suitable microenvironment for wound healing and to serve as a sustainable therapeutic.
34907778	2	26	theme	wound	510:514	arg1	healing					516:522	accelerating wound healing	497:522	accelerating wound healing	497:522	Hence, we developed a stimuli-responsive drug-loaded hydrogel wound dressing for sustained, controlled release of the drug and accelerating wound healing.
34907778	4	27	theme	various	960:966	arg1	media					976:980	various release media	960:980	various release media	960:980	Experimental results indicated that these hydrogel composites showed pH and temperature stimuli-responsive behaviors and released the encapsulated drug sustainedly in various release media.
34907778	2	28	theme	accelerating	497:508	arg1	healing					516:522	accelerating wound healing	497:522	accelerating wound healing	497:522	Hence, we developed a stimuli-responsive drug-loaded hydrogel wound dressing for sustained, controlled release of the drug and accelerating wound healing.
34907778	3	29	theme	substitution	651:662	arg1	degrees					640:646	various degrees	632:646	various degrees	632:646	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	7	30	theme	hydrogel	1440:1447	arg1	dressings					1449:1457	Rh-EGF-loaded CMA hydrogel dressings	1422:1457	Rh-EGF-loaded CMA hydrogel dressings	1422:1457	These results confirm that Rh-EGF-loaded CMA hydrogel dressings possess potential application in accelerating wound healing and tissue regeneration.
34907778	0	31	theme	Multistimuli-Responsive	27:49	arg1	Dressing					60:67	Agarose-Based Multistimuli-Responsive Hydrogel Dressing	13:67	Agarose-Based Multistimuli-Responsive Hydrogel Dressing	13:67	Synthesis of Agarose-Based Multistimuli-Responsive Hydrogel Dressing for Accelerated Wound Healing.
34907778	1	32	from	interest	160:167	arg1	medicine					185:192	regenerative medicine	172:192	regenerative medicine	172:192	Stimuli-responsive hydrogels have drawn increasing research interest in regenerative medicine due to their tunable molecular structures and functional properties for both providing a suitable microenvironment for wound healing and to serve as a sustainable therapeutic.
34907778	3	33	theme	various	632:638	arg1	degrees					640:646	various degrees	632:646	various degrees	632:646	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	1	34	theme	functional	240:249	arg1	properties					251:260	functional properties	240:260	functional properties for both providing a suitable microenvironment for wound healing and to serve as a sustainable therapeutic	240:367	Stimuli-responsive hydrogels have drawn increasing research interest in regenerative medicine due to their tunable molecular structures and functional properties for both providing a suitable microenvironment for wound healing and to serve as a sustainable therapeutic.
34907778	2	35	theme	drug	488:491	arg1	release					473:479	sustained, controlled release	451:479	sustained, controlled release of the drug	451:491	Hence, we developed a stimuli-responsive drug-loaded hydrogel wound dressing for sustained, controlled release of the drug and accelerating wound healing.
34907778	2	35	theme	drug	488:491	arg1	healing					516:522	accelerating wound healing	497:522	accelerating wound healing	497:522	Hence, we developed a stimuli-responsive drug-loaded hydrogel wound dressing for sustained, controlled release of the drug and accelerating wound healing.
34907778	3	36	theme	recombinant	721:731	arg1	Rh-EGF					764:769	Rh-EGF	764:769	Rh-EGF	764:769	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	3	36	theme	recombinant	721:731	arg1	factor					756:761	recombinant human epidermal growth factor	721:761	recombinant human epidermal growth factor (Rh-EGF)	721:770	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	1	37	theme	increasing	140:149	arg1	interest					160:167	increasing research interest	140:167	increasing research interest in regenerative medicine	140:192	Stimuli-responsive hydrogels have drawn increasing research interest in regenerative medicine due to their tunable molecular structures and functional properties for both providing a suitable microenvironment for wound healing and to serve as a sustainable therapeutic.
34907778	3	38	theme	carboxymethyl	599:611	arg1	CMA					622:624	CMA	622:624	CMA	622:624	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	3	38	theme	carboxymethyl	599:611	arg1	agarose					613:619	carboxymethyl agarose	599:619	carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel	599:790	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	0	39	theme	Dressing	60:67	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Agarose-Based Multistimuli-Responsive Hydrogel Dressing for Accelerated Wound Healing.	0:98	Synthesis of Agarose-Based Multistimuli-Responsive Hydrogel Dressing for Accelerated Wound Healing.
34907778	6	40	theme	inflammatory	1241:1252	arg1	responses					1254:1262	inflammatory responses	1241:1262	inflammatory responses	1241:1262	The investigation in vivo showed that the Rh-EGF-loaded CMA hydrogel dressing significantly enhanced wound healing and reduced inflammatory responses by upregulating the transforming growth factor-beta, vascular endothelial growth factor, and cluster of differentiation 31 (CD31).
34907778	3	41	theme	human	733:737	arg1	Rh-EGF					764:769	Rh-EGF	764:769	Rh-EGF	764:769	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	3	41	theme	human	733:737	arg1	factor					756:761	recombinant human epidermal growth factor	721:761	recombinant human epidermal growth factor (Rh-EGF)	721:770	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	3	42	theme	ion	676:678	arg1	crosslinking					680:691	calcium ion crosslinking	668:691	calcium ion crosslinking	668:691	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	6	43	theme	Rh-EGF-loaded	1156:1168	arg1	dressing					1183:1190	the Rh-EGF-loaded CMA hydrogel dressing	1152:1190	the Rh-EGF-loaded CMA hydrogel dressing	1152:1190	The investigation in vivo showed that the Rh-EGF-loaded CMA hydrogel dressing significantly enhanced wound healing and reduced inflammatory responses by upregulating the transforming growth factor-beta, vascular endothelial growth factor, and cluster of differentiation 31 (CD31).
34907778	0	44	theme	Hydrogel	51:58	arg1	Dressing					60:67	Agarose-Based Multistimuli-Responsive Hydrogel Dressing	13:67	Agarose-Based Multistimuli-Responsive Hydrogel Dressing	13:67	Synthesis of Agarose-Based Multistimuli-Responsive Hydrogel Dressing for Accelerated Wound Healing.
34907778	3	45	with	dressings	534:542	arg1	properties					568:577	stimuli-responsive properties	549:577	stimuli-responsive properties	549:577	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	3	46	theme	crosslinking	680:691	arg1	degrees					640:646	various degrees	632:646	various degrees	632:646	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	0	47	theme	Accelerated	73:83	arg1	Healing					91:97	Accelerated Wound Healing	73:97	Accelerated Wound Healing	73:97	Synthesis of Agarose-Based Multistimuli-Responsive Hydrogel Dressing for Accelerated Wound Healing.
34907778	7	48	contain	possess	1459:1465	arg1	dressings					1449:1457	Rh-EGF-loaded CMA hydrogel dressings	1422:1457	Rh-EGF-loaded CMA hydrogel dressings	1422:1457	These results confirm that Rh-EGF-loaded CMA hydrogel dressings possess potential application in accelerating wound healing and tissue regeneration.
34907778	7	48	contain	possess	1459:1465	arg2	application					1477:1487	potential application	1467:1487	potential application	1467:1487	These results confirm that Rh-EGF-loaded CMA hydrogel dressings possess potential application in accelerating wound healing and tissue regeneration.
34907778	4	49	theme	encapsulated	927:938	arg1	sustainedly					945:955	the encapsulated drug sustainedly	923:955	the encapsulated drug sustainedly in various release media	923:980	Experimental results indicated that these hydrogel composites showed pH and temperature stimuli-responsive behaviors and released the encapsulated drug sustainedly in various release media.
34907778	4	50	from	sustainedly	945:955	arg1	media					976:980	various release media	960:980	various release media	960:980	Experimental results indicated that these hydrogel composites showed pH and temperature stimuli-responsive behaviors and released the encapsulated drug sustainedly in various release media.
34907778	1	51	theme	research	151:158	arg1	interest					160:167	increasing research interest	140:167	increasing research interest in regenerative medicine	140:192	Stimuli-responsive hydrogels have drawn increasing research interest in regenerative medicine due to their tunable molecular structures and functional properties for both providing a suitable microenvironment for wound healing and to serve as a sustainable therapeutic.
34907778	6	52	theme	growth	1338:1343	arg1	factor					1345:1350	vascular endothelial growth factor	1317:1350	vascular endothelial growth factor	1317:1350	The investigation in vivo showed that the Rh-EGF-loaded CMA hydrogel dressing significantly enhanced wound healing and reduced inflammatory responses by upregulating the transforming growth factor-beta, vascular endothelial growth factor, and cluster of differentiation 31 (CD31).
34907778	3	53	theme	factor	756:761	arg1	loading					710:716	the loading	706:716	the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel	706:790	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	3	54	theme	calcium	668:674	arg1	crosslinking					680:691	calcium ion crosslinking	668:691	calcium ion crosslinking	668:691	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	7	55	theme	wound	1505:1509	arg1	healing					1511:1517	wound healing	1505:1517	wound healing	1505:1517	These results confirm that Rh-EGF-loaded CMA hydrogel dressings possess potential application in accelerating wound healing and tissue regeneration.
34907778	7	56	theme	CMA	1436:1438	arg1	dressings					1449:1457	Rh-EGF-loaded CMA hydrogel dressings	1422:1457	Rh-EGF-loaded CMA hydrogel dressings	1422:1457	These results confirm that Rh-EGF-loaded CMA hydrogel dressings possess potential application in accelerating wound healing and tissue regeneration.
34907778	6	57	theme	differentiation	1368:1382	arg1	factor-beta					1304:1314	the transforming growth factor-beta	1280:1314	the transforming growth factor-beta	1280:1314	The investigation in vivo showed that the Rh-EGF-loaded CMA hydrogel dressing significantly enhanced wound healing and reduced inflammatory responses by upregulating the transforming growth factor-beta, vascular endothelial growth factor, and cluster of differentiation 31 (CD31).
34907778	6	57	theme	differentiation	1368:1382	arg1	cluster					1357:1363	cluster	1357:1363	cluster of differentiation 31 (CD31)	1357:1392	The investigation in vivo showed that the Rh-EGF-loaded CMA hydrogel dressing significantly enhanced wound healing and reduced inflammatory responses by upregulating the transforming growth factor-beta, vascular endothelial growth factor, and cluster of differentiation 31 (CD31).
34907778	6	57	theme	differentiation	1368:1382	arg1	CD31					1388:1391	CD31	1388:1391	CD31	1388:1391	The investigation in vivo showed that the Rh-EGF-loaded CMA hydrogel dressing significantly enhanced wound healing and reduced inflammatory responses by upregulating the transforming growth factor-beta, vascular endothelial growth factor, and cluster of differentiation 31 (CD31).
34907778	6	57	theme	differentiation	1368:1382	arg1	factor					1345:1350	vascular endothelial growth factor	1317:1350	vascular endothelial growth factor	1317:1350	The investigation in vivo showed that the Rh-EGF-loaded CMA hydrogel dressing significantly enhanced wound healing and reduced inflammatory responses by upregulating the transforming growth factor-beta, vascular endothelial growth factor, and cluster of differentiation 31 (CD31).
34907778	2	58	dep	sustained	451:459	arg1	controlled					462:471	controlled	462:471	controlled	462:471	Hence, we developed a stimuli-responsive drug-loaded hydrogel wound dressing for sustained, controlled release of the drug and accelerating wound healing.
34907778	7	59	theme	tissue	1523:1528	arg1	regeneration					1530:1541	tissue regeneration	1523:1541	tissue regeneration	1523:1541	These results confirm that Rh-EGF-loaded CMA hydrogel dressings possess potential application in accelerating wound healing and tissue regeneration.
34907778	4	60	theme	Experimental	793:804	arg1	results					806:812	Experimental results	793:812	Experimental results	793:812	Experimental results indicated that these hydrogel composites showed pH and temperature stimuli-responsive behaviors and released the encapsulated drug sustainedly in various release media.
34907778	5	61	theme	stable	1054:1059	arg1	properties					1070:1079	stable physical properties	1054:1079	stable physical properties	1054:1079	Moreover, the hydrogel dressings exhibited a porous network structure, stable physical properties, and excellent biocompatibility.
34907778	5	62	theme	excellent	1086:1094	arg1	biocompatibility					1096:1111	excellent biocompatibility	1086:1111	excellent biocompatibility	1086:1111	Moreover, the hydrogel dressings exhibited a porous network structure, stable physical properties, and excellent biocompatibility.
34907778	3	63	theme	epidermal	739:747	arg1	Rh-EGF					764:769	Rh-EGF	764:769	Rh-EGF	764:769	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	3	63	theme	epidermal	739:747	arg1	factor					756:761	recombinant human epidermal growth factor	721:761	recombinant human epidermal growth factor (Rh-EGF)	721:770	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	1	64	theme	regenerative	172:183	arg1	medicine					185:192	regenerative medicine	172:192	regenerative medicine	172:192	Stimuli-responsive hydrogels have drawn increasing research interest in regenerative medicine due to their tunable molecular structures and functional properties for both providing a suitable microenvironment for wound healing and to serve as a sustainable therapeutic.
34907778	5	65	theme	network	1035:1041	arg1	structure					1043:1051	a porous network structure	1026:1051	a porous network structure	1026:1051	Moreover, the hydrogel dressings exhibited a porous network structure, stable physical properties, and excellent biocompatibility.
34907778	3	66	theme	growth	749:754	arg1	Rh-EGF					764:769	Rh-EGF	764:769	Rh-EGF	764:769	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	3	66	theme	growth	749:754	arg1	factor					756:761	recombinant human epidermal growth factor	721:761	recombinant human epidermal growth factor (Rh-EGF)	721:770	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	3	67	theme	Hydrogel	525:532	arg1	dressings					534:542	Hydrogel dressings	525:542	Hydrogel dressings with stimuli-responsive properties	525:577	Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel.
34907778	2	68	theme	wound	432:436	arg1	dressing					438:445	a stimuli-responsive drug-loaded hydrogel wound dressing	390:445	a stimuli-responsive drug-loaded hydrogel wound dressing for sustained, controlled release of the drug and accelerating wound healing	390:522	Hence, we developed a stimuli-responsive drug-loaded hydrogel wound dressing for sustained, controlled release of the drug and accelerating wound healing.
34907778	2	69	theme	sustained	451:459	arg1	release					473:479	sustained, controlled release	451:479	sustained, controlled release of the drug	451:491	Hence, we developed a stimuli-responsive drug-loaded hydrogel wound dressing for sustained, controlled release of the drug and accelerating wound healing.
34907778	1	70	theme	suitable	283:290	arg1	microenvironment					292:307	a suitable microenvironment	281:307	a suitable microenvironment for wound healing	281:325	Stimuli-responsive hydrogels have drawn increasing research interest in regenerative medicine due to their tunable molecular structures and functional properties for both providing a suitable microenvironment for wound healing and to serve as a sustainable therapeutic.
34907778	0	71	theme	Wound	85:89	arg1	Healing					91:97	Accelerated Wound Healing	73:97	Accelerated Wound Healing	73:97	Synthesis of Agarose-Based Multistimuli-Responsive Hydrogel Dressing for Accelerated Wound Healing.
34907778	6	72	theme	transforming	1284:1295	arg1	factor-beta					1304:1314	the transforming growth factor-beta	1280:1314	the transforming growth factor-beta	1280:1314	The investigation in vivo showed that the Rh-EGF-loaded CMA hydrogel dressing significantly enhanced wound healing and reduced inflammatory responses by upregulating the transforming growth factor-beta, vascular endothelial growth factor, and cluster of differentiation 31 (CD31).
34907778	0	73	theme	Agarose-Based	13:25	arg1	Dressing					60:67	Agarose-Based Multistimuli-Responsive Hydrogel Dressing	13:67	Agarose-Based Multistimuli-Responsive Hydrogel Dressing	13:67	Synthesis of Agarose-Based Multistimuli-Responsive Hydrogel Dressing for Accelerated Wound Healing.
34907778	2	74	theme	hydrogel	423:430	arg1	dressing					438:445	a stimuli-responsive drug-loaded hydrogel wound dressing	390:445	a stimuli-responsive drug-loaded hydrogel wound dressing for sustained, controlled release of the drug and accelerating wound healing	390:522	Hence, we developed a stimuli-responsive drug-loaded hydrogel wound dressing for sustained, controlled release of the drug and accelerating wound healing.
34872779	0	0	theme	dyes	81:84	arg1	adsorption					67:76	the adsorption	63:76	the adsorption of dyes	63:84	Synthesis of chitosan composite of metal-organic framework for the adsorption of dyes; kinetic and thermodynamic approach.
34872779	2	1	theme	MOF-235	421:427	arg1	chitosan					433:440	chitosan	433:440	chitosan	433:440	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	2	1	theme	MOF-235	421:427	arg1	MOF-235					421:427	MOF-235	421:427	MOF-235	421:427	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	2	1	theme	MOF-235	421:427	arg1	framework					397:405	the iron metal-organic framework (CS/MOF-235)	374:418	the iron metal-organic framework (CS/MOF-235)	374:418	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	2	1	theme	MOF-235	421:427	arg1	composite					361:369	The chitosan composite	348:369	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan	348:440	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	1	2	theme	composite	156:164	arg1	framework					146:154	The iron metal-organic framework composite	123:164	The iron metal-organic framework composite with chitosan (CS/MOF-235)	123:191	The iron metal-organic framework composite with chitosan (CS/MOF-235) was synthesized using a solvothermal method and its synthesis was confirmed by surface area, PZC, XRD, FESEM, XPS, TGA, TEM, EDX mapping and EDX analysis.
34872779	3	3	theme	greater	763:769	arg1	capacity					715:722	the adsorption capacity	700:722	the adsorption capacity of CS/MOF-235	700:736	The maximum adsorption capacities were found to be 2857-2326 mg/g for CS/MOF-235, 357 - 236 mg/g for MOF-235 and 209-171 mg/g for chitosan (CS) which reveal that the adsorption capacity of CS/MOF-235 is almost 8 and 14 times greater than MOF-235 and chitosan respectively.
34872779	3	3	theme	greater	763:769	arg1	times					757:761	8 and 14 times	748:761	8 and 14 times greater than MOF-235 and chitosan respectively	748:808	The maximum adsorption capacities were found to be 2857-2326 mg/g for CS/MOF-235, 357 - 236 mg/g for MOF-235 and 209-171 mg/g for chitosan (CS) which reveal that the adsorption capacity of CS/MOF-235 is almost 8 and 14 times greater than MOF-235 and chitosan respectively.
34872779	0	4	dep	approach	113:120	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of chitosan composite of metal-organic framework for the adsorption of dyes	0:84	Synthesis of chitosan composite of metal-organic framework for the adsorption of dyes; kinetic and thermodynamic approach.
34872779	7	5	theme	dyes	1390:1393	arg1	uptake					1380:1385	the greater uptake	1368:1385	the greater uptake of dyes	1368:1393	The negative values of the isosteric heat of adsorption (ΔH¯) fall with surface coverage indicating that a lesser amount of heat is required for the greater uptake of dyes.
34872779	0	6	theme	kinetic	87:93	arg1	approach					113:120	kinetic and thermodynamic approach	87:120	kinetic and thermodynamic approach	87:120	Synthesis of chitosan composite of metal-organic framework for the adsorption of dyes; kinetic and thermodynamic approach.
34872779	2	7	theme	orange	502:507	arg1	removal					460:466	the removal	456:466	the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions	456:535	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	7	8	theme	surface	1295:1301	arg1	coverage					1303:1310	surface coverage	1295:1310	surface coverage indicating that a lesser amount of heat is required for the greater uptake of dyes	1295:1393	The negative values of the isosteric heat of adsorption (ΔH¯) fall with surface coverage indicating that a lesser amount of heat is required for the greater uptake of dyes.
34872779	2	9	theme	methyl	495:500	arg1	MO					510:511	MO	510:511	MO	510:511	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	2	9	theme	methyl	495:500	arg1	orange					502:507	methyl orange	495:507	methyl orange (MO)	495:512	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	6	10	theme	adsorption	1149:1158	arg1	process					1160:1166	the adsorption process	1145:1166	the adsorption process	1145:1166	The intraparticle diffusion and Richenberg models confirmed that the adsorption process was jointly controlled by the pore and film diffusion.
34872779	3	11	theme	adsorption	704:713	arg1	times					757:761	8 and 14 times	748:761	8 and 14 times greater than MOF-235 and chitosan respectively	748:808	The maximum adsorption capacities were found to be 2857-2326 mg/g for CS/MOF-235, 357 - 236 mg/g for MOF-235 and 209-171 mg/g for chitosan (CS) which reveal that the adsorption capacity of CS/MOF-235 is almost 8 and 14 times greater than MOF-235 and chitosan respectively.
34872779	3	11	theme	adsorption	704:713	arg1	capacity					715:722	the adsorption capacity	700:722	the adsorption capacity of CS/MOF-235	700:736	The maximum adsorption capacities were found to be 2857-2326 mg/g for CS/MOF-235, 357 - 236 mg/g for MOF-235 and 209-171 mg/g for chitosan (CS) which reveal that the adsorption capacity of CS/MOF-235 is almost 8 and 14 times greater than MOF-235 and chitosan respectively.
34872779	0	12	theme	thermodynamic	99:111	arg1	approach					113:120	kinetic and thermodynamic approach	87:120	kinetic and thermodynamic approach	87:120	Synthesis of chitosan composite of metal-organic framework for the adsorption of dyes; kinetic and thermodynamic approach.
34872779	7	13	theme	lesser	1330:1335	arg1	heat					1347:1350	heat	1347:1350	heat	1347:1350	The negative values of the isosteric heat of adsorption (ΔH¯) fall with surface coverage indicating that a lesser amount of heat is required for the greater uptake of dyes.
34872779	7	13	theme	lesser	1330:1335	arg1	amount					1337:1342	a lesser amount	1328:1342	a lesser amount of heat	1328:1350	The negative values of the isosteric heat of adsorption (ΔH¯) fall with surface coverage indicating that a lesser amount of heat is required for the greater uptake of dyes.
34872779	2	14	theme	framework	397:405	arg1	chitosan					433:440	chitosan	433:440	chitosan	433:440	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	2	14	theme	framework	397:405	arg1	MOF-235					421:427	MOF-235	421:427	MOF-235	421:427	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	2	14	theme	framework	397:405	arg1	framework					397:405	the iron metal-organic framework (CS/MOF-235)	374:418	the iron metal-organic framework (CS/MOF-235)	374:418	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	2	14	theme	framework	397:405	arg1	composite					361:369	The chitosan composite	348:369	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan	348:440	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	5	15	theme	electrostatic	957:969	arg1	chemisorption					988:1000	chemisorption	988:1000	chemisorption	988:1000	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	15	theme	electrostatic	957:969	arg1	pore-filling					943:954	pore-filling	943:954	pore-filling	943:954	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	15	theme	electrostatic	957:969	arg1	bonding					919:925	hydrogen bonding	910:925	hydrogen bonding	910:925	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	15	theme	electrostatic	957:969	arg1	interactions					971:982	electrostatic interactions	957:982	electrostatic interactions	957:982	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	15	theme	electrostatic	957:969	arg1	mechanisms					1028:1037	possible mechanisms	1019:1037	possible mechanisms for the removal of dyes onto CS/MOF-235	1019:1077	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	15	theme	electrostatic	957:969	arg1	bonding					934:940	pi-pi bonding	928:940	pi-pi bonding	928:940	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	2	16	theme	aqueous	519:525	arg1	solutions					527:535	aqueous solutions	519:535	aqueous solutions	519:535	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	4	17	theme	order	885:889	arg1	> MB					894:897	the order MO > MB	881:897	the order MO > MB	881:897	The adsorption selectivity of the (CS/MOF-235) towards the dye was in the order MO > MB.
34872779	2	18	theme	metal-organic	383:395	arg1	framework					397:405	the iron metal-organic framework (CS/MOF-235)	374:418	the iron metal-organic framework (CS/MOF-235)	374:418	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	1	19	theme	EDX	318:320	arg1	mapping					322:328	EDX mapping	318:328	EDX mapping	318:328	The iron metal-organic framework composite with chitosan (CS/MOF-235) was synthesized using a solvothermal method and its synthesis was confirmed by surface area, PZC, XRD, FESEM, XPS, TGA, TEM, EDX mapping and EDX analysis.
34872779	1	19	theme	EDX	318:320	arg1	area					280:283	surface area	272:283	surface area	272:283	The iron metal-organic framework composite with chitosan (CS/MOF-235) was synthesized using a solvothermal method and its synthesis was confirmed by surface area, PZC, XRD, FESEM, XPS, TGA, TEM, EDX mapping and EDX analysis.
34872779	1	20	with	framework	146:154	arg1	chitosan					171:178	chitosan (CS/MOF-235)	171:191	chitosan (CS/MOF-235)	171:191	The iron metal-organic framework composite with chitosan (CS/MOF-235) was synthesized using a solvothermal method and its synthesis was confirmed by surface area, PZC, XRD, FESEM, XPS, TGA, TEM, EDX mapping and EDX analysis.
34872779	2	21	theme	iron	378:381	arg1	framework					397:405	the iron metal-organic framework (CS/MOF-235)	374:418	the iron metal-organic framework (CS/MOF-235)	374:418	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	1	22	theme	surface	272:278	arg1	analysis					338:345	EDX analysis	334:345	EDX analysis	334:345	The iron metal-organic framework composite with chitosan (CS/MOF-235) was synthesized using a solvothermal method and its synthesis was confirmed by surface area, PZC, XRD, FESEM, XPS, TGA, TEM, EDX mapping and EDX analysis.
34872779	1	22	theme	surface	272:278	arg1	TEM					313:315	TEM	313:315	TEM	313:315	The iron metal-organic framework composite with chitosan (CS/MOF-235) was synthesized using a solvothermal method and its synthesis was confirmed by surface area, PZC, XRD, FESEM, XPS, TGA, TEM, EDX mapping and EDX analysis.
34872779	1	22	theme	surface	272:278	arg1	area					280:283	surface area	272:283	surface area	272:283	The iron metal-organic framework composite with chitosan (CS/MOF-235) was synthesized using a solvothermal method and its synthesis was confirmed by surface area, PZC, XRD, FESEM, XPS, TGA, TEM, EDX mapping and EDX analysis.
34872779	1	22	theme	surface	272:278	arg1	PZC					286:288	PZC	286:288	PZC	286:288	The iron metal-organic framework composite with chitosan (CS/MOF-235) was synthesized using a solvothermal method and its synthesis was confirmed by surface area, PZC, XRD, FESEM, XPS, TGA, TEM, EDX mapping and EDX analysis.
34872779	1	22	theme	surface	272:278	arg1	XPS					303:305	XPS	303:305	XPS	303:305	The iron metal-organic framework composite with chitosan (CS/MOF-235) was synthesized using a solvothermal method and its synthesis was confirmed by surface area, PZC, XRD, FESEM, XPS, TGA, TEM, EDX mapping and EDX analysis.
34872779	1	22	theme	surface	272:278	arg1	mapping					322:328	EDX mapping	318:328	EDX mapping	318:328	The iron metal-organic framework composite with chitosan (CS/MOF-235) was synthesized using a solvothermal method and its synthesis was confirmed by surface area, PZC, XRD, FESEM, XPS, TGA, TEM, EDX mapping and EDX analysis.
34872779	1	22	theme	surface	272:278	arg1	TGA					308:310	TGA	308:310	TGA	308:310	The iron metal-organic framework composite with chitosan (CS/MOF-235) was synthesized using a solvothermal method and its synthesis was confirmed by surface area, PZC, XRD, FESEM, XPS, TGA, TEM, EDX mapping and EDX analysis.
34872779	3	23	theme	adsorption	550:559	arg1	2857-2326 mg/g					589:602	2857-2326 mg/g	589:602	2857-2326 mg/g for CS/MOF-235, 357 - 236 mg/g for MOF-235 and 209-171 mg/g for chitosan (CS) which reveal that the adsorption capacity of CS/MOF-235 is almost 8 and 14 times greater than MOF-235 and chitosan respectively	589:808	The maximum adsorption capacities were found to be 2857-2326 mg/g for CS/MOF-235, 357 - 236 mg/g for MOF-235 and 209-171 mg/g for chitosan (CS) which reveal that the adsorption capacity of CS/MOF-235 is almost 8 and 14 times greater than MOF-235 and chitosan respectively.
34872779	3	23	theme	adsorption	550:559	arg1	capacities					561:570	The maximum adsorption capacities	538:570	The maximum adsorption capacities	538:570	The maximum adsorption capacities were found to be 2857-2326 mg/g for CS/MOF-235, 357 - 236 mg/g for MOF-235 and 209-171 mg/g for chitosan (CS) which reveal that the adsorption capacity of CS/MOF-235 is almost 8 and 14 times greater than MOF-235 and chitosan respectively.
34872779	0	24	theme	composite	22:30	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of chitosan composite of metal-organic framework for the adsorption of dyes	0:84	Synthesis of chitosan composite of metal-organic framework for the adsorption of dyes; kinetic and thermodynamic approach.
34872779	2	25	theme	blue	481:484	arg1	methylene					471:479	methylene blue	471:484	methylene blue (MB)	471:489	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	2	25	theme	blue	481:484	arg1	MB					487:488	MB	487:488	MB	487:488	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	7	26	theme	isosteric	1250:1258	arg1	heat					1260:1263	the isosteric heat	1246:1263	the isosteric heat of adsorption (ΔH¯)	1246:1283	The negative values of the isosteric heat of adsorption (ΔH¯) fall with surface coverage indicating that a lesser amount of heat is required for the greater uptake of dyes.
34872779	7	27	theme	adsorption	1268:1277	arg1	heat					1260:1263	the isosteric heat	1246:1263	the isosteric heat of adsorption (ΔH¯)	1246:1283	The negative values of the isosteric heat of adsorption (ΔH¯) fall with surface coverage indicating that a lesser amount of heat is required for the greater uptake of dyes.
34872779	7	28	theme	heat	1260:1263	arg1	values					1236:1241	The negative values	1223:1241	The negative values of the isosteric heat of adsorption (ΔH¯)	1223:1283	The negative values of the isosteric heat of adsorption (ΔH¯) fall with surface coverage indicating that a lesser amount of heat is required for the greater uptake of dyes.
34872779	0	29	theme	chitosan	13:20	arg1	composite					22:30	chitosan composite	13:30	chitosan composite of metal-organic framework for the adsorption of dyes	13:84	Synthesis of chitosan composite of metal-organic framework for the adsorption of dyes; kinetic and thermodynamic approach.
34872779	0	29	theme	chitosan	13:20	arg1	framework					49:57	metal-organic framework	35:57	metal-organic framework for the adsorption of dyes	35:84	Synthesis of chitosan composite of metal-organic framework for the adsorption of dyes; kinetic and thermodynamic approach.
34872779	2	30	theme	methylene	471:479	arg1	removal					460:466	the removal	456:466	the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions	456:535	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	3	31	theme	maximum	542:548	arg1	2857-2326 mg/g					589:602	2857-2326 mg/g	589:602	2857-2326 mg/g for CS/MOF-235, 357 - 236 mg/g for MOF-235 and 209-171 mg/g for chitosan (CS) which reveal that the adsorption capacity of CS/MOF-235 is almost 8 and 14 times greater than MOF-235 and chitosan respectively	589:808	The maximum adsorption capacities were found to be 2857-2326 mg/g for CS/MOF-235, 357 - 236 mg/g for MOF-235 and 209-171 mg/g for chitosan (CS) which reveal that the adsorption capacity of CS/MOF-235 is almost 8 and 14 times greater than MOF-235 and chitosan respectively.
34872779	3	31	theme	maximum	542:548	arg1	capacities					561:570	The maximum adsorption capacities	538:570	The maximum adsorption capacities	538:570	The maximum adsorption capacities were found to be 2857-2326 mg/g for CS/MOF-235, 357 - 236 mg/g for MOF-235 and 209-171 mg/g for chitosan (CS) which reveal that the adsorption capacity of CS/MOF-235 is almost 8 and 14 times greater than MOF-235 and chitosan respectively.
34872779	2	32	theme	chitosan	352:359	arg1	chitosan					433:440	chitosan	433:440	chitosan	433:440	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	2	32	theme	chitosan	352:359	arg1	MOF-235					421:427	MOF-235	421:427	MOF-235	421:427	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	2	32	theme	chitosan	352:359	arg1	framework					397:405	the iron metal-organic framework (CS/MOF-235)	374:418	the iron metal-organic framework (CS/MOF-235)	374:418	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	2	32	theme	chitosan	352:359	arg1	composite					361:369	The chitosan composite	348:369	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan	348:440	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	0	33	theme	metal-organic	35:47	arg1	framework					49:57	metal-organic framework	35:57	metal-organic framework for the adsorption of dyes	35:84	Synthesis of chitosan composite of metal-organic framework for the adsorption of dyes; kinetic and thermodynamic approach.
34872779	5	34	theme	dyes	1058:1061	arg1	removal					1047:1053	the removal	1043:1053	the removal of dyes onto CS/MOF-235	1043:1077	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	4	35	theme	adsorption	815:824	arg1	selectivity					826:836	The adsorption selectivity	811:836	The adsorption selectivity of the (CS/MOF-235) towards the dye	811:872	The adsorption selectivity of the (CS/MOF-235) towards the dye was in the order MO > MB.
34872779	2	36	used	used	447:450	arg2	composite					361:369	The chitosan composite	348:369	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan	348:440	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	2	36	used	used	447:450	arg2	chitosan					433:440	chitosan	433:440	chitosan	433:440	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	2	36	used	used	447:450	arg2	framework					397:405	the iron metal-organic framework (CS/MOF-235)	374:418	the iron metal-organic framework (CS/MOF-235)	374:418	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	2	36	used	used	447:450	arg2	MOF-235					421:427	MOF-235	421:427	MOF-235	421:427	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	6	37	theme	Richenberg	1112:1121	arg1	models					1123:1128	The intraparticle diffusion and Richenberg models	1080:1128	models	1123:1128	The intraparticle diffusion and Richenberg models confirmed that the adsorption process was jointly controlled by the pore and film diffusion.
34872779	1	38	theme	EDX	334:336	arg1	analysis					338:345	EDX analysis	334:345	EDX analysis	334:345	The iron metal-organic framework composite with chitosan (CS/MOF-235) was synthesized using a solvothermal method and its synthesis was confirmed by surface area, PZC, XRD, FESEM, XPS, TGA, TEM, EDX mapping and EDX analysis.
34872779	1	38	theme	EDX	334:336	arg1	area					280:283	surface area	272:283	surface area	272:283	The iron metal-organic framework composite with chitosan (CS/MOF-235) was synthesized using a solvothermal method and its synthesis was confirmed by surface area, PZC, XRD, FESEM, XPS, TGA, TEM, EDX mapping and EDX analysis.
34872779	5	39	theme	possible	1019:1026	arg1	chemisorption					988:1000	chemisorption	988:1000	chemisorption	988:1000	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	39	theme	possible	1019:1026	arg1	pore-filling					943:954	pore-filling	943:954	pore-filling	943:954	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	39	theme	possible	1019:1026	arg1	bonding					919:925	hydrogen bonding	910:925	hydrogen bonding	910:925	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	39	theme	possible	1019:1026	arg1	interactions					971:982	electrostatic interactions	957:982	electrostatic interactions	957:982	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	39	theme	possible	1019:1026	arg1	mechanisms					1028:1037	possible mechanisms	1019:1037	possible mechanisms for the removal of dyes onto CS/MOF-235	1019:1077	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	39	theme	possible	1019:1026	arg1	bonding					934:940	pi-pi bonding	928:940	pi-pi bonding	928:940	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	6	40	theme	pore	1198:1201	arg1	diffusion					1212:1220	the pore and film diffusion	1194:1220	diffusion	1212:1220	The intraparticle diffusion and Richenberg models confirmed that the adsorption process was jointly controlled by the pore and film diffusion.
34872779	6	41	theme	film	1207:1210	arg1	diffusion					1212:1220	the pore and film diffusion	1194:1220	diffusion	1212:1220	The intraparticle diffusion and Richenberg models confirmed that the adsorption process was jointly controlled by the pore and film diffusion.
34872779	0	42	theme	framework	49:57	arg1	composite					22:30	chitosan composite	13:30	chitosan composite of metal-organic framework for the adsorption of dyes	13:84	Synthesis of chitosan composite of metal-organic framework for the adsorption of dyes; kinetic and thermodynamic approach.
34872779	0	42	theme	framework	49:57	arg1	framework					49:57	metal-organic framework	35:57	metal-organic framework for the adsorption of dyes	35:84	Synthesis of chitosan composite of metal-organic framework for the adsorption of dyes; kinetic and thermodynamic approach.
34872779	2	43	from	solutions	527:535	arg1	removal					460:466	the removal	456:466	the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions	456:535	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	6	44	theme	intraparticle	1084:1096	arg1	diffusion					1098:1106	The intraparticle diffusion and Richenberg models	1080:1128	diffusion	1098:1106	The intraparticle diffusion and Richenberg models confirmed that the adsorption process was jointly controlled by the pore and film diffusion.
34872779	7	45	theme	greater	1372:1378	arg1	uptake					1380:1385	the greater uptake	1368:1385	the greater uptake of dyes	1368:1393	The negative values of the isosteric heat of adsorption (ΔH¯) fall with surface coverage indicating that a lesser amount of heat is required for the greater uptake of dyes.
34872779	1	46	theme	solvothermal	217:228	arg1	method					230:235	a solvothermal method	215:235	a solvothermal method	215:235	The iron metal-organic framework composite with chitosan (CS/MOF-235) was synthesized using a solvothermal method and its synthesis was confirmed by surface area, PZC, XRD, FESEM, XPS, TGA, TEM, EDX mapping and EDX analysis.
34872779	5	47	theme	hydrogen	910:917	arg1	chemisorption					988:1000	chemisorption	988:1000	chemisorption	988:1000	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	47	theme	hydrogen	910:917	arg1	pore-filling					943:954	pore-filling	943:954	pore-filling	943:954	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	47	theme	hydrogen	910:917	arg1	bonding					919:925	hydrogen bonding	910:925	hydrogen bonding	910:925	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	47	theme	hydrogen	910:917	arg1	interactions					971:982	electrostatic interactions	957:982	electrostatic interactions	957:982	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	47	theme	hydrogen	910:917	arg1	mechanisms					1028:1037	possible mechanisms	1019:1037	possible mechanisms for the removal of dyes onto CS/MOF-235	1019:1077	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	47	theme	hydrogen	910:917	arg1	bonding					934:940	pi-pi bonding	928:940	pi-pi bonding	928:940	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	48	theme	pi-pi	928:932	arg1	chemisorption					988:1000	chemisorption	988:1000	chemisorption	988:1000	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	48	theme	pi-pi	928:932	arg1	pore-filling					943:954	pore-filling	943:954	pore-filling	943:954	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	48	theme	pi-pi	928:932	arg1	bonding					919:925	hydrogen bonding	910:925	hydrogen bonding	910:925	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	48	theme	pi-pi	928:932	arg1	interactions					971:982	electrostatic interactions	957:982	electrostatic interactions	957:982	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	48	theme	pi-pi	928:932	arg1	mechanisms					1028:1037	possible mechanisms	1019:1037	possible mechanisms for the removal of dyes onto CS/MOF-235	1019:1077	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	5	48	theme	pi-pi	928:932	arg1	bonding					934:940	pi-pi bonding	928:940	pi-pi bonding	928:940	Moreover, hydrogen bonding, pi-pi bonding, pore-filling, electrostatic interactions and chemisorption were proposed as possible mechanisms for the removal of dyes onto CS/MOF-235.
34872779	1	49	theme	iron	127:130	arg1	framework					146:154	The iron metal-organic framework composite	123:164	The iron metal-organic framework composite with chitosan (CS/MOF-235)	123:191	The iron metal-organic framework composite with chitosan (CS/MOF-235) was synthesized using a solvothermal method and its synthesis was confirmed by surface area, PZC, XRD, FESEM, XPS, TGA, TEM, EDX mapping and EDX analysis.
34872779	3	50	theme	CS/MOF-235	727:736	arg1	times					757:761	8 and 14 times	748:761	8 and 14 times greater than MOF-235 and chitosan respectively	748:808	The maximum adsorption capacities were found to be 2857-2326 mg/g for CS/MOF-235, 357 - 236 mg/g for MOF-235 and 209-171 mg/g for chitosan (CS) which reveal that the adsorption capacity of CS/MOF-235 is almost 8 and 14 times greater than MOF-235 and chitosan respectively.
34872779	3	50	theme	CS/MOF-235	727:736	arg1	capacity					715:722	the adsorption capacity	700:722	the adsorption capacity of CS/MOF-235	700:736	The maximum adsorption capacities were found to be 2857-2326 mg/g for CS/MOF-235, 357 - 236 mg/g for MOF-235 and 209-171 mg/g for chitosan (CS) which reveal that the adsorption capacity of CS/MOF-235 is almost 8 and 14 times greater than MOF-235 and chitosan respectively.
34872779	2	51	theme	chitosan	433:440	arg1	chitosan					433:440	chitosan	433:440	chitosan	433:440	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	2	51	theme	chitosan	433:440	arg1	MOF-235					421:427	MOF-235	421:427	MOF-235	421:427	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	2	51	theme	chitosan	433:440	arg1	framework					397:405	the iron metal-organic framework (CS/MOF-235)	374:418	the iron metal-organic framework (CS/MOF-235)	374:418	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	2	51	theme	chitosan	433:440	arg1	composite					361:369	The chitosan composite	348:369	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan	348:440	The chitosan composite of the iron metal-organic framework (CS/MOF-235), MOF-235 and chitosan were used for the removal of methylene blue (MB) and methyl orange (MO) from aqueous solutions.
34872779	1	52	theme	metal-organic	132:144	arg1	framework					146:154	The iron metal-organic framework composite	123:164	The iron metal-organic framework composite with chitosan (CS/MOF-235)	123:191	The iron metal-organic framework composite with chitosan (CS/MOF-235) was synthesized using a solvothermal method and its synthesis was confirmed by surface area, PZC, XRD, FESEM, XPS, TGA, TEM, EDX mapping and EDX analysis.
34872779	4	53	theme	CS/MOF-235	846:855	arg1	selectivity					826:836	The adsorption selectivity	811:836	The adsorption selectivity of the (CS/MOF-235) towards the dye	811:872	The adsorption selectivity of the (CS/MOF-235) towards the dye was in the order MO > MB.
34872779	7	54	theme	heat	1347:1350	arg1	heat					1347:1350	heat	1347:1350	heat	1347:1350	The negative values of the isosteric heat of adsorption (ΔH¯) fall with surface coverage indicating that a lesser amount of heat is required for the greater uptake of dyes.
34872779	7	54	theme	heat	1347:1350	arg1	amount					1337:1342	a lesser amount	1328:1342	a lesser amount of heat	1328:1350	The negative values of the isosteric heat of adsorption (ΔH¯) fall with surface coverage indicating that a lesser amount of heat is required for the greater uptake of dyes.
34872779	7	55	theme	negative	1227:1234	arg1	values					1236:1241	The negative values	1223:1241	The negative values of the isosteric heat of adsorption (ΔH¯)	1223:1283	The negative values of the isosteric heat of adsorption (ΔH¯) fall with surface coverage indicating that a lesser amount of heat is required for the greater uptake of dyes.
34872779	4	56	theme	MO	891:892	arg1	> MB					894:897	the order MO > MB	881:897	the order MO > MB	881:897	The adsorption selectivity of the (CS/MOF-235) towards the dye was in the order MO > MB.
32297468	4	0	theme	hemolytic	1017:1025	arg1	ratio					1027:1031	the hemolytic ratio	1013:1031	the hemolytic ratio of less than 2%	1013:1047	In vitro hemocompatibility study proves that the Cu-HAP/CS/PVP composites are blood compatible with the hemolytic ratio of less than 2%.
32297468	4	1	dep	In	913:914	arg1	vitro					916:920	vitro	916:920	vitro	916:920	In vitro hemocompatibility study proves that the Cu-HAP/CS/PVP composites are blood compatible with the hemolytic ratio of less than 2%.
32297468	5	2	theme	Cu-HAP/CS/PVP	1128:1140	arg1	composite					1142:1150	the optimized Cu-HAP/CS/PVP composite	1114:1150	the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP)	1114:1169	In vitro bioactivity study revealed the formation of apatite on the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP) in simulated body fluid (SBF) solution.
32297468	4	3	theme	In	913:914	arg1	study					940:944	In vitro hemocompatibility study	913:944	In vitro hemocompatibility study	913:944	In vitro hemocompatibility study proves that the Cu-HAP/CS/PVP composites are blood compatible with the hemolytic ratio of less than 2%.
32297468	3	4	theme	Candida	848:854	arg1	albicans					856:863	Candida albicans	848:863	Candida albicans	848:863	In vitro antimicrobial activity was investigated against bacteria (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli) and fungus (Candida albicans, Penicillium notatum, and Rhizopus stolonifer).
32297468	7	5	theme	4,5-Dimethylthiazol-2-yl	1443:1466	arg1	assay					1501:1505	MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-DiphenyltetrazoliumBromide) assay	1435:1505	MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-DiphenyltetrazoliumBromide) assay	1435:1505	In vitro biocompatibility was studied against human osteosarcoma cell line by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-DiphenyltetrazoliumBromide) assay.
32297468	3	6	theme	In	705:706	arg1	activity					728:735	In vitro antimicrobial activity	705:735	In vitro antimicrobial activity	705:735	In vitro antimicrobial activity was investigated against bacteria (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli) and fungus (Candida albicans, Penicillium notatum, and Rhizopus stolonifer).
32297468	5	7	theme	apatite	1103:1109	arg1	formation					1090:1098	the formation	1086:1098	the formation of apatite	1086:1109	In vitro bioactivity study revealed the formation of apatite on the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP) in simulated body fluid (SBF) solution.
32297468	1	8	theme	copper-hydroxyapatite	246:266	arg1	%					240:240	0, 20, 40, 60, and 80 wt%	216:240	0, 20, 40, 60, and 80 wt%	216:240	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	1	8	theme	copper-hydroxyapatite	246:266	arg1	ratios					205:210	different weight ratios	188:210	different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP)	188:275	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	6	9	theme	ions	1338:1341	arg1	leaching					1312:1319	the leaching	1308:1319	the leaching of Ca, P, and Cu ions from the SBF	1308:1354	ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis was used to find out the leaching of Ca, P, and Cu ions from the SBF.
32297468	6	10	from	SBF	1352:1354	arg1	leaching					1312:1319	the leaching	1308:1319	the leaching of Ca, P, and Cu ions from the SBF	1308:1354	ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis was used to find out the leaching of Ca, P, and Cu ions from the SBF.
32297468	7	11	theme	human	1403:1407	arg1	line					1427:1430	human osteosarcoma cell line	1403:1430	human osteosarcoma cell line	1403:1430	In vitro biocompatibility was studied against human osteosarcoma cell line by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-DiphenyltetrazoliumBromide) assay.
32297468	3	12	theme	Rhizopus	891:898	arg1	stolonifer					900:909	Rhizopus stolonifer	891:909	Rhizopus stolonifer	891:909	In vitro antimicrobial activity was investigated against bacteria (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli) and fungus (Candida albicans, Penicillium notatum, and Rhizopus stolonifer).
32297468	6	13	used	used	1291:1294	arg2	analysis					1278:1285	ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis	1211:1285	ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis	1211:1285	ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis was used to find out the leaching of Ca, P, and Cu ions from the SBF.
32297468	2	14	theme	%	548:548	arg1	Cu-HAP					550:555	80 wt% Cu-HAP	543:555	80 wt% Cu-HAP	543:555	The Cu-HAP/CS/PVP composite with 80 wt% Cu-HAP showed 98.73 ± 1.14% of porosity with highest tensile strength (101.45 ± 0.98 MPa) and less swelling percentage (19.51 ± 1.03%) compared to others.
32297468	1	15	theme	X-ray	348:352	arg1	diffraction					354:364	X-ray diffraction	348:364	X-ray diffraction	348:364	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	2	16	with	composite	528:536	arg1	Cu-HAP					550:555	80 wt% Cu-HAP	543:555	80 wt% Cu-HAP	543:555	The Cu-HAP/CS/PVP composite with 80 wt% Cu-HAP showed 98.73 ± 1.14% of porosity with highest tensile strength (101.45 ± 0.98 MPa) and less swelling percentage (19.51 ± 1.03%) compared to others.
32297468	7	17	theme	osteosarcoma	1409:1420	arg1	line					1427:1430	human osteosarcoma cell line	1403:1430	human osteosarcoma cell line	1403:1430	In vitro biocompatibility was studied against human osteosarcoma cell line by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-DiphenyltetrazoliumBromide) assay.
32297468	7	18	theme	-2,5-DiphenyltetrazoliumBromide	1468:1498	arg1	assay					1501:1505	MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-DiphenyltetrazoliumBromide) assay	1435:1505	MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-DiphenyltetrazoliumBromide) assay	1435:1505	In vitro biocompatibility was studied against human osteosarcoma cell line by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-DiphenyltetrazoliumBromide) assay.
32297468	2	19	theme	80 wt	543:547	arg1	Cu-HAP					550:555	80 wt% Cu-HAP	543:555	80 wt% Cu-HAP	543:555	The Cu-HAP/CS/PVP composite with 80 wt% Cu-HAP showed 98.73 ± 1.14% of porosity with highest tensile strength (101.45 ± 0.98 MPa) and less swelling percentage (19.51 ± 1.03%) compared to others.
32297468	7	20	theme	MTT	1435:1437	arg1	assay					1501:1505	MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-DiphenyltetrazoliumBromide) assay	1435:1505	MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-DiphenyltetrazoliumBromide) assay	1435:1505	In vitro biocompatibility was studied against human osteosarcoma cell line by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-DiphenyltetrazoliumBromide) assay.
32297468	5	21	theme	optimized	1118:1126	arg1	composite					1142:1150	the optimized Cu-HAP/CS/PVP composite	1114:1150	the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP)	1114:1169	In vitro bioactivity study revealed the formation of apatite on the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP) in simulated body fluid (SBF) solution.
32297468	2	22	theme	swelling	649:656	arg1	%					682:682	19.51 ± 1.03%	670:682	19.51 ± 1.03%	670:682	The Cu-HAP/CS/PVP composite with 80 wt% Cu-HAP showed 98.73 ± 1.14% of porosity with highest tensile strength (101.45 ± 0.98 MPa) and less swelling percentage (19.51 ± 1.03%) compared to others.
32297468	2	22	theme	swelling	649:656	arg1	percentage					658:667	less swelling percentage	644:667	less swelling percentage (19.51 ± 1.03%)	644:683	The Cu-HAP/CS/PVP composite with 80 wt% Cu-HAP showed 98.73 ± 1.14% of porosity with highest tensile strength (101.45 ± 0.98 MPa) and less swelling percentage (19.51 ± 1.03%) compared to others.
32297468	1	23	theme	tricomponent	120:131	arg1	CS/PVP					175:180	CS/PVP	175:180	CS/PVP	175:180	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	1	23	theme	tricomponent	120:131	arg1	pyrrolidone					162:172	The tricomponent composite chitosan/polyvinyl pyrrolidone	116:172	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP)	116:275	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	5	24	dep	In	1050:1051	arg1	vitro					1053:1057	vitro	1053:1057	vitro	1053:1057	In vitro bioactivity study revealed the formation of apatite on the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP) in simulated body fluid (SBF) solution.
32297468	1	25	with	pyrrolidone	162:172	arg1	%					240:240	0, 20, 40, 60, and 80 wt%	216:240	0, 20, 40, 60, and 80 wt%	216:240	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	1	25	with	pyrrolidone	162:172	arg1	ratios					205:210	different weight ratios	188:210	different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP)	188:275	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	1	26	theme	Fourier-transform	367:383	arg1	infrared					385:392	Fourier-transform infrared	367:392	Fourier-transform infrared	367:392	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	6	27	theme	Emission	1255:1262	arg1	Spectroscopy					1264:1275	Inductively Coupled Plasma-Optical Emission Spectroscopy	1220:1275	Inductively Coupled Plasma-Optical Emission Spectroscopy	1220:1275	ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis was used to find out the leaching of Ca, P, and Cu ions from the SBF.
32297468	6	27	theme	Emission	1255:1262	arg1	ICP-OES					1211:1217	ICP-OES	1211:1217	ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis	1211:1285	ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis was used to find out the leaching of Ca, P, and Cu ions from the SBF.
32297468	5	28	theme	simulated	1174:1182	arg1	SBF					1196:1198	SBF	1196:1198	SBF	1196:1198	In vitro bioactivity study revealed the formation of apatite on the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP) in simulated body fluid (SBF) solution.
32297468	5	28	theme	simulated	1174:1182	arg1	fluid					1189:1193	simulated body fluid	1174:1193	simulated body fluid (SBF) solution	1174:1208	In vitro bioactivity study revealed the formation of apatite on the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP) in simulated body fluid (SBF) solution.
32297468	1	29	theme	composite	133:141	arg1	CS/PVP					175:180	CS/PVP	175:180	CS/PVP	175:180	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	1	29	theme	composite	133:141	arg1	pyrrolidone					162:172	The tricomponent composite chitosan/polyvinyl pyrrolidone	116:172	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP)	116:275	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	0	30	theme	Tricomponent	0:11	arg1	composite					13:21	Tricomponent composite	0:21	Tricomponent composite	0:21	Tricomponent composite containing copper-hydroxyapatite/chitosan/polyvinyl pyrrolidone for bone tissue engineering.
32297468	3	31	dep	In	705:706	arg1	vitro					708:712	vitro	708:712	vitro	708:712	In vitro antimicrobial activity was investigated against bacteria (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli) and fungus (Candida albicans, Penicillium notatum, and Rhizopus stolonifer).
32297468	6	32	theme	Plasma-Optical	1240:1253	arg1	Spectroscopy					1264:1275	Inductively Coupled Plasma-Optical Emission Spectroscopy	1220:1275	Inductively Coupled Plasma-Optical Emission Spectroscopy	1220:1275	ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis was used to find out the leaching of Ca, P, and Cu ions from the SBF.
32297468	6	32	theme	Plasma-Optical	1240:1253	arg1	ICP-OES					1211:1217	ICP-OES	1211:1217	ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis	1211:1285	ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis was used to find out the leaching of Ca, P, and Cu ions from the SBF.
32297468	1	33	theme	Cu-HAP/CS/PVP	485:497	arg1	composite					499:507	Cu-HAP/CS/PVP composite	485:507	Cu-HAP/CS/PVP composite	485:507	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	1	34	theme	chitosan/polyvinyl	143:160	arg1	CS/PVP					175:180	CS/PVP	175:180	CS/PVP	175:180	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	1	34	theme	chitosan/polyvinyl	143:160	arg1	pyrrolidone					162:172	The tricomponent composite chitosan/polyvinyl pyrrolidone	116:172	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP)	116:275	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	0	35	theme	copper-hydroxyapatite/chitosan/polyvinyl	34:73	arg1	pyrrolidone					75:85	copper-hydroxyapatite/chitosan/polyvinyl pyrrolidone	34:85	copper-hydroxyapatite/chitosan/polyvinyl pyrrolidone for bone tissue engineering	34:113	Tricomponent composite containing copper-hydroxyapatite/chitosan/polyvinyl pyrrolidone for bone tissue engineering.
32297468	5	36	theme	body	1184:1187	arg1	SBF					1196:1198	SBF	1196:1198	SBF	1196:1198	In vitro bioactivity study revealed the formation of apatite on the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP) in simulated body fluid (SBF) solution.
32297468	5	36	theme	body	1184:1187	arg1	fluid					1189:1193	simulated body fluid	1174:1193	simulated body fluid (SBF) solution	1174:1208	In vitro bioactivity study revealed the formation of apatite on the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP) in simulated body fluid (SBF) solution.
32297468	6	37	theme	P	1328:1328	arg1	ions					1338:1341	Ca, P, and Cu ions	1324:1341	Ca, P, and Cu ions	1324:1341	ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis was used to find out the leaching of Ca, P, and Cu ions from the SBF.
32297468	1	38	theme	composite	499:507	arg1	formation					472:480	the formation	468:480	the formation of Cu-HAP/CS/PVP composite	468:507	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	7	39	theme	In	1357:1358	arg1	biocompatibility					1366:1381	In vitro biocompatibility	1357:1381	In vitro biocompatibility	1357:1381	In vitro biocompatibility was studied against human osteosarcoma cell line by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-DiphenyltetrazoliumBromide) assay.
32297468	5	40	theme	fluid	1189:1193	arg1	solution					1201:1208	simulated body fluid (SBF) solution	1174:1208	simulated body fluid (SBF) solution	1174:1208	In vitro bioactivity study revealed the formation of apatite on the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP) in simulated body fluid (SBF) solution.
32297468	1	41	dep	%	240:240	arg1	of					212:213	of	212:213	of	212:213	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	5	42	dep	composite	1142:1150	arg1	Cu-HAP					1163:1168	Cu-HAP	1163:1168	Cu-HAP	1163:1168	In vitro bioactivity study revealed the formation of apatite on the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP) in simulated body fluid (SBF) solution.
32297468	5	42	dep	composite	1142:1150	arg1	%					1158:1158	80 wt%	1153:1158	80 wt% of Cu-HAP	1153:1168	In vitro bioactivity study revealed the formation of apatite on the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP) in simulated body fluid (SBF) solution.
32297468	7	43	dep	In	1357:1358	arg1	vitro					1360:1364	vitro	1360:1364	vitro	1360:1364	In vitro biocompatibility was studied against human osteosarcoma cell line by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-DiphenyltetrazoliumBromide) assay.
32297468	6	44	theme	Ca	1324:1325	arg1	ions					1338:1341	Ca, P, and Cu ions	1324:1341	Ca, P, and Cu ions	1324:1341	ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis was used to find out the leaching of Ca, P, and Cu ions from the SBF.
32297468	6	45	theme	Coupled	1232:1238	arg1	Spectroscopy					1264:1275	Inductively Coupled Plasma-Optical Emission Spectroscopy	1220:1275	Inductively Coupled Plasma-Optical Emission Spectroscopy	1220:1275	ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis was used to find out the leaching of Ca, P, and Cu ions from the SBF.
32297468	6	45	theme	Coupled	1232:1238	arg1	ICP-OES					1211:1217	ICP-OES	1211:1217	ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis	1211:1285	ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis was used to find out the leaching of Ca, P, and Cu ions from the SBF.
32297468	1	46	theme	solvent	294:300	arg1	technique					310:318	solvent casting technique	294:318	solvent casting technique	294:318	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	0	47	theme	tissue	96:101	arg1	engineering					103:113	bone tissue engineering	91:113	bone tissue engineering	91:113	Tricomponent composite containing copper-hydroxyapatite/chitosan/polyvinyl pyrrolidone for bone tissue engineering.
32297468	1	48	theme	casting	302:308	arg1	technique					310:318	solvent casting technique	294:318	solvent casting technique	294:318	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	2	49	theme	tensile	603:609	arg1	101.45 ± 0.98 MPa					621:637	101.45 ± 0.98 MPa	621:637	101.45 ± 0.98 MPa	621:637	The Cu-HAP/CS/PVP composite with 80 wt% Cu-HAP showed 98.73 ± 1.14% of porosity with highest tensile strength (101.45 ± 0.98 MPa) and less swelling percentage (19.51 ± 1.03%) compared to others.
32297468	2	49	theme	tensile	603:609	arg1	strength					611:618	highest tensile strength	595:618	highest tensile strength (101.45 ± 0.98 MPa)	595:638	The Cu-HAP/CS/PVP composite with 80 wt% Cu-HAP showed 98.73 ± 1.14% of porosity with highest tensile strength (101.45 ± 0.98 MPa) and less swelling percentage (19.51 ± 1.03%) compared to others.
32297468	1	50	theme	scanning	399:406	arg1	microscopy					417:426	scanning electron microscopy	399:426	scanning electron microscopy	399:426	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	7	51	theme	cell	1422:1425	arg1	line					1427:1430	human osteosarcoma cell line	1403:1430	human osteosarcoma cell line	1403:1430	In vitro biocompatibility was studied against human osteosarcoma cell line by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-DiphenyltetrazoliumBromide) assay.
32297468	5	52	theme	80 wt	1153:1157	arg1	Cu-HAP					1163:1168	Cu-HAP	1163:1168	Cu-HAP	1163:1168	In vitro bioactivity study revealed the formation of apatite on the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP) in simulated body fluid (SBF) solution.
32297468	5	52	theme	80 wt	1153:1157	arg1	%					1158:1158	80 wt%	1153:1158	80 wt% of Cu-HAP	1153:1168	In vitro bioactivity study revealed the formation of apatite on the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP) in simulated body fluid (SBF) solution.
32297468	0	53	theme	bone	91:94	arg1	engineering					103:113	bone tissue engineering	91:113	bone tissue engineering	91:113	Tricomponent composite containing copper-hydroxyapatite/chitosan/polyvinyl pyrrolidone for bone tissue engineering.
32297468	3	54	dep	fungus	840:845	arg1	notatum					878:884	Penicillium notatum	866:884	Penicillium notatum	866:884	In vitro antimicrobial activity was investigated against bacteria (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli) and fungus (Candida albicans, Penicillium notatum, and Rhizopus stolonifer).
32297468	3	54	dep	fungus	840:845	arg1	stolonifer					900:909	Rhizopus stolonifer	891:909	Rhizopus stolonifer	891:909	In vitro antimicrobial activity was investigated against bacteria (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli) and fungus (Candida albicans, Penicillium notatum, and Rhizopus stolonifer).
32297468	3	54	dep	fungus	840:845	arg1	albicans					856:863	Candida albicans	848:863	Candida albicans	848:863	In vitro antimicrobial activity was investigated against bacteria (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli) and fungus (Candida albicans, Penicillium notatum, and Rhizopus stolonifer).
32297468	2	55	theme	highest	595:601	arg1	101.45 ± 0.98 MPa					621:637	101.45 ± 0.98 MPa	621:637	101.45 ± 0.98 MPa	621:637	The Cu-HAP/CS/PVP composite with 80 wt% Cu-HAP showed 98.73 ± 1.14% of porosity with highest tensile strength (101.45 ± 0.98 MPa) and less swelling percentage (19.51 ± 1.03%) compared to others.
32297468	2	55	theme	highest	595:601	arg1	strength					611:618	highest tensile strength	595:618	highest tensile strength (101.45 ± 0.98 MPa)	595:638	The Cu-HAP/CS/PVP composite with 80 wt% Cu-HAP showed 98.73 ± 1.14% of porosity with highest tensile strength (101.45 ± 0.98 MPa) and less swelling percentage (19.51 ± 1.03%) compared to others.
32297468	1	56	theme	electron	408:415	arg1	microscopy					417:426	scanning electron microscopy	399:426	scanning electron microscopy	399:426	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	5	57	theme	In	1050:1051	arg1	study					1071:1075	In vitro bioactivity study	1050:1075	In vitro bioactivity study	1050:1075	In vitro bioactivity study revealed the formation of apatite on the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP) in simulated body fluid (SBF) solution.
32297468	6	58	theme	ICP-OES	1211:1217	arg1	analysis					1278:1285	ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis	1211:1285	ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis	1211:1285	ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis was used to find out the leaching of Ca, P, and Cu ions from the SBF.
32297468	3	59	dep	bacteria	762:769	arg1	aureus					787:792	Staphylococcus aureus	772:792	Staphylococcus aureus	772:792	In vitro antimicrobial activity was investigated against bacteria (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli) and fungus (Candida albicans, Penicillium notatum, and Rhizopus stolonifer).
32297468	3	59	dep	bacteria	762:769	arg1	subtilis					804:811	Bacillus subtilis	795:811	Bacillus subtilis	795:811	In vitro antimicrobial activity was investigated against bacteria (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli) and fungus (Candida albicans, Penicillium notatum, and Rhizopus stolonifer).
32297468	3	59	dep	bacteria	762:769	arg1	coli					830:833	Escherichia coli	818:833	Escherichia coli	818:833	In vitro antimicrobial activity was investigated against bacteria (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli) and fungus (Candida albicans, Penicillium notatum, and Rhizopus stolonifer).
32297468	4	60	theme	hemocompatibility	922:938	arg1	study					940:944	In vitro hemocompatibility study	913:944	In vitro hemocompatibility study	913:944	In vitro hemocompatibility study proves that the Cu-HAP/CS/PVP composites are blood compatible with the hemolytic ratio of less than 2%.
32297468	5	61	theme	bioactivity	1059:1069	arg1	study					1071:1075	In vitro bioactivity study	1050:1075	In vitro bioactivity study	1050:1075	In vitro bioactivity study revealed the formation of apatite on the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP) in simulated body fluid (SBF) solution.
32297468	4	62	with	compatible	997:1006	arg1	ratio					1027:1031	the hemolytic ratio	1013:1031	the hemolytic ratio of less than 2%	1013:1047	In vitro hemocompatibility study proves that the Cu-HAP/CS/PVP composites are blood compatible with the hemolytic ratio of less than 2%.
32297468	2	63	theme	porosity	581:588	arg1	%					576:576	98.73 ± 1.14%	564:576	98.73 ± 1.14% of porosity	564:588	The Cu-HAP/CS/PVP composite with 80 wt% Cu-HAP showed 98.73 ± 1.14% of porosity with highest tensile strength (101.45 ± 0.98 MPa) and less swelling percentage (19.51 ± 1.03%) compared to others.
32297468	2	63	theme	porosity	581:588	arg1	porosity					581:588	porosity	581:588	porosity	581:588	The Cu-HAP/CS/PVP composite with 80 wt% Cu-HAP showed 98.73 ± 1.14% of porosity with highest tensile strength (101.45 ± 0.98 MPa) and less swelling percentage (19.51 ± 1.03%) compared to others.
32297468	1	64	theme	different	188:196	arg1	%					240:240	0, 20, 40, 60, and 80 wt%	216:240	0, 20, 40, 60, and 80 wt%	216:240	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	1	64	theme	different	188:196	arg1	ratios					205:210	different weight ratios	188:210	different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP)	188:275	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	6	65	theme	Cu	1335:1336	arg1	ions					1338:1341	Ca, P, and Cu ions	1324:1341	Ca, P, and Cu ions	1324:1341	ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis was used to find out the leaching of Ca, P, and Cu ions from the SBF.
32297468	1	66	theme	80 wt	235:239	arg1	%					240:240	0, 20, 40, 60, and 80 wt%	216:240	0, 20, 40, 60, and 80 wt%	216:240	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	1	66	theme	80 wt	235:239	arg1	ratios					205:210	different weight ratios	188:210	different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP)	188:275	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	5	67	theme	Cu-HAP	1163:1168	arg1	Cu-HAP					1163:1168	Cu-HAP	1163:1168	Cu-HAP	1163:1168	In vitro bioactivity study revealed the formation of apatite on the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP) in simulated body fluid (SBF) solution.
32297468	5	67	theme	Cu-HAP	1163:1168	arg1	%					1158:1158	80 wt%	1153:1158	80 wt% of Cu-HAP	1153:1168	In vitro bioactivity study revealed the formation of apatite on the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP) in simulated body fluid (SBF) solution.
32297468	1	68	theme	energy-dispersive	433:449	arg1	X-ray					451:455	energy-dispersive X-ray	433:455	energy-dispersive X-ray	433:455	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	1	69	theme	weight	198:203	arg1	%					240:240	0, 20, 40, 60, and 80 wt%	216:240	0, 20, 40, 60, and 80 wt%	216:240	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	1	69	theme	weight	198:203	arg1	ratios					205:210	different weight ratios	188:210	different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP)	188:275	The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite.
32297468	3	70	theme	Penicillium	866:876	arg1	notatum					878:884	Penicillium notatum	866:884	Penicillium notatum	866:884	In vitro antimicrobial activity was investigated against bacteria (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli) and fungus (Candida albicans, Penicillium notatum, and Rhizopus stolonifer).
32297468	2	71	theme	Cu-HAP/CS/PVP	514:526	arg1	composite					528:536	The Cu-HAP/CS/PVP composite	510:536	The Cu-HAP/CS/PVP composite with 80 wt% Cu-HAP	510:555	The Cu-HAP/CS/PVP composite with 80 wt% Cu-HAP showed 98.73 ± 1.14% of porosity with highest tensile strength (101.45 ± 0.98 MPa) and less swelling percentage (19.51 ± 1.03%) compared to others.
32297468	3	72	theme	antimicrobial	714:726	arg1	activity					728:735	In vitro antimicrobial activity	705:735	In vitro antimicrobial activity	705:735	In vitro antimicrobial activity was investigated against bacteria (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli) and fungus (Candida albicans, Penicillium notatum, and Rhizopus stolonifer).
32297468	4	73	theme	%	1047:1047	arg1	ratio					1027:1031	the hemolytic ratio	1013:1031	the hemolytic ratio of less than 2%	1013:1047	In vitro hemocompatibility study proves that the Cu-HAP/CS/PVP composites are blood compatible with the hemolytic ratio of less than 2%.
32297468	4	74	theme	Cu-HAP/CS/PVP	962:974	arg1	composites					976:985	the Cu-HAP/CS/PVP composites	958:985	the Cu-HAP/CS/PVP composites	958:985	In vitro hemocompatibility study proves that the Cu-HAP/CS/PVP composites are blood compatible with the hemolytic ratio of less than 2%.
32297468	4	74	theme	Cu-HAP/CS/PVP	962:974	arg1	compatible					997:1006	compatible	997:1006	compatible	997:1006	In vitro hemocompatibility study proves that the Cu-HAP/CS/PVP composites are blood compatible with the hemolytic ratio of less than 2%.
34856218	7	0	theme	films	1097:1101	arg1	application					1113:1123	the composite films potential application	1083:1123	the composite films potential application	1083:1123	The current study exhibited that PS-CNPs films were more effective in inhibiting bacteria growth than neat PS film, confirming the composite films potential application as antimicrobial food packaging.
34856218	1	1	theme	antibacterial	273:285	arg1	agent					287:291	a reinforcing and antibacterial agent	255:291	a reinforcing and antibacterial agent	255:291	This study aimed to develop a plasticized starch (PS) based film loaded with chitosan nanoparticles (CNPs, 1, 2, 3, and 4%) as a reinforcing and antibacterial agent.
34856218	1	2	theme	starch	170:175	arg1	film					188:191	a plasticized starch (PS) based film	156:191	a plasticized starch (PS) based film loaded with chitosan nanoparticles (CNPs, 1, 2, 3, and 4%) as a reinforcing and antibacterial agent	156:291	This study aimed to develop a plasticized starch (PS) based film loaded with chitosan nanoparticles (CNPs, 1, 2, 3, and 4%) as a reinforcing and antibacterial agent.
34856218	0	3	theme	nanocomposites	78:91	arg1	attributes					57:66	Biodegradability, physical, mechanical and antimicrobial attributes	0:66	Biodegradability, physical, mechanical and antimicrobial attributes of starch nanocomposites	0:91	Biodegradability, physical, mechanical and antimicrobial attributes of starch nanocomposites containing chitosan nanoparticles.
34856218	6	4	theme	PS-CNPs	820:826	arg1	films					838:842	PS-CNPs composite films	820:842	PS-CNPs composite films	820:842	PS-CNPs composite films revealed inhibition zones against both E. coli and S. aureus, with the 100% reduction in CFU against S. aureus.
34856218	5	5	theme	barrier	800:806	arg1	properties					808:817	barrier properties	800:817	barrier properties	800:817	The increase in the CNPs percentage led to improved mechanical behaviour and barrier properties.
34856218	3	6	dep	bacteria	510:517	arg1	coli					504:507	E. coli	501:507	E. coli	501:507	The antimicrobial activity against both Gram-positive (S. aureus) and Gram-negative (E. coli) bacteria was investigated by colony forming unit (CFU) and disc diffusion methods.
34856218	6	7	from	reduction	920:928	arg1	CFU					933:935	CFU	933:935	CFU against S. aureus	933:953	PS-CNPs composite films revealed inhibition zones against both E. coli and S. aureus, with the 100% reduction in CFU against S. aureus.
34856218	3	8	theme	Gram-negative	486:498	arg1	bacteria					510:517	both Gram-positive (S. aureus) and Gram-negative (E. coli) bacteria	451:517	bacteria	510:517	The antimicrobial activity against both Gram-positive (S. aureus) and Gram-negative (E. coli) bacteria was investigated by colony forming unit (CFU) and disc diffusion methods.
34856218	0	9	theme	chitosan	104:111	arg1	nanoparticles					113:125	chitosan nanoparticles	104:125	chitosan nanoparticles	104:125	Biodegradability, physical, mechanical and antimicrobial attributes of starch nanocomposites containing chitosan nanoparticles.
34856218	4	10	dep	obtained	615:622	arg1	occurred					687:694	occurred	687:694	occurred in more days than neat PS	687:720	A dense structure was obtained for all PS/CNPs films and, thus, their complete biodegradation occurred in more days than neat PS.
34856218	4	11	theme	complete	663:670	arg1	biodegradation					672:685	their complete biodegradation	657:685	their complete biodegradation	657:685	A dense structure was obtained for all PS/CNPs films and, thus, their complete biodegradation occurred in more days than neat PS.
34856218	5	12	from	increase	727:734	arg1	percentage					748:757	the CNPs percentage	739:757	the CNPs percentage	739:757	The increase in the CNPs percentage led to improved mechanical behaviour and barrier properties.
34856218	7	13	theme	neat	1058:1061	arg1	film					1066:1069	neat PS film	1058:1069	neat PS film	1058:1069	The current study exhibited that PS-CNPs films were more effective in inhibiting bacteria growth than neat PS film, confirming the composite films potential application as antimicrobial food packaging.
34856218	2	14	dep	morphology	310:319	arg1	the					306:308	the	306:308	the	306:308	We examined the morphology, biodegradability, mechanical, thermo-mechanical, and barrier properties of the PS/CNPs films.
34856218	4	15	theme	dense	595:599	arg1	structure					601:609	A dense structure	593:609	A dense structure	593:609	A dense structure was obtained for all PS/CNPs films and, thus, their complete biodegradation occurred in more days than neat PS.
34856218	1	16	theme	PS	178:179	arg1	film					188:191	a plasticized starch (PS) based film	156:191	a plasticized starch (PS) based film loaded with chitosan nanoparticles (CNPs, 1, 2, 3, and 4%) as a reinforcing and antibacterial agent	156:291	This study aimed to develop a plasticized starch (PS) based film loaded with chitosan nanoparticles (CNPs, 1, 2, 3, and 4%) as a reinforcing and antibacterial agent.
34856218	2	17	theme	PS/CNPs	401:407	arg1	films					409:413	the PS/CNPs films	397:413	the PS/CNPs films	397:413	We examined the morphology, biodegradability, mechanical, thermo-mechanical, and barrier properties of the PS/CNPs films.
34856218	7	18	theme	PS-CNPs	989:995	arg1	films					997:1001	PS-CNPs films	989:1001	PS-CNPs films	989:1001	The current study exhibited that PS-CNPs films were more effective in inhibiting bacteria growth than neat PS film, confirming the composite films potential application as antimicrobial food packaging.
34856218	7	19	theme	PS	1063:1064	arg1	film					1066:1069	neat PS film	1058:1069	neat PS film	1058:1069	The current study exhibited that PS-CNPs films were more effective in inhibiting bacteria growth than neat PS film, confirming the composite films potential application as antimicrobial food packaging.
34856218	7	20	theme	potential	1103:1111	arg1	application					1113:1123	the composite films potential application	1083:1123	the composite films potential application	1083:1123	The current study exhibited that PS-CNPs films were more effective in inhibiting bacteria growth than neat PS film, confirming the composite films potential application as antimicrobial food packaging.
34856218	3	21	dep	Gram-positive	456:468	arg1	aureus					474:479	S. aureus	471:479	S. aureus	471:479	The antimicrobial activity against both Gram-positive (S. aureus) and Gram-negative (E. coli) bacteria was investigated by colony forming unit (CFU) and disc diffusion methods.
34856218	1	22	theme	based	182:186	arg1	film					188:191	a plasticized starch (PS) based film	156:191	a plasticized starch (PS) based film loaded with chitosan nanoparticles (CNPs, 1, 2, 3, and 4%) as a reinforcing and antibacterial agent	156:291	This study aimed to develop a plasticized starch (PS) based film loaded with chitosan nanoparticles (CNPs, 1, 2, 3, and 4%) as a reinforcing and antibacterial agent.
34856218	0	23	theme	Biodegradability	0:15	arg1	attributes					57:66	Biodegradability, physical, mechanical and antimicrobial attributes	0:66	Biodegradability, physical, mechanical and antimicrobial attributes of starch nanocomposites	0:91	Biodegradability, physical, mechanical and antimicrobial attributes of starch nanocomposites containing chitosan nanoparticles.
34856218	5	24	theme	mechanical	775:784	arg1	behaviour					786:794	improved mechanical behaviour	766:794	improved mechanical behaviour	766:794	The increase in the CNPs percentage led to improved mechanical behaviour and barrier properties.
34856218	5	25	theme	CNPs	743:746	arg1	percentage					748:757	the CNPs percentage	739:757	the CNPs percentage	739:757	The increase in the CNPs percentage led to improved mechanical behaviour and barrier properties.
34856218	2	26	theme	barrier	375:381	arg1	properties					383:392	barrier properties	375:392	barrier properties	375:392	We examined the morphology, biodegradability, mechanical, thermo-mechanical, and barrier properties of the PS/CNPs films.
34856218	7	27	theme	current	960:966	arg1	study					968:972	The current study	956:972	The current study	956:972	The current study exhibited that PS-CNPs films were more effective in inhibiting bacteria growth than neat PS film, confirming the composite films potential application as antimicrobial food packaging.
34856218	3	28	theme	disc	569:572	arg1	methods					584:590	disc diffusion methods	569:590	disc diffusion methods	569:590	The antimicrobial activity against both Gram-positive (S. aureus) and Gram-negative (E. coli) bacteria was investigated by colony forming unit (CFU) and disc diffusion methods.
34856218	7	29	theme	food	1142:1145	arg1	packaging					1147:1155	antimicrobial food packaging	1128:1155	antimicrobial food packaging	1128:1155	The current study exhibited that PS-CNPs films were more effective in inhibiting bacteria growth than neat PS film, confirming the composite films potential application as antimicrobial food packaging.
34856218	6	30	theme	composite	828:836	arg1	films					838:842	PS-CNPs composite films	820:842	PS-CNPs composite films	820:842	PS-CNPs composite films revealed inhibition zones against both E. coli and S. aureus, with the 100% reduction in CFU against S. aureus.
34856218	7	31	theme	bacteria	1037:1044	arg1	growth					1046:1051	bacteria growth	1037:1051	bacteria growth than neat PS film	1037:1069	The current study exhibited that PS-CNPs films were more effective in inhibiting bacteria growth than neat PS film, confirming the composite films potential application as antimicrobial food packaging.
34856218	3	32	theme	diffusion	574:582	arg1	methods					584:590	disc diffusion methods	569:590	disc diffusion methods	569:590	The antimicrobial activity against both Gram-positive (S. aureus) and Gram-negative (E. coli) bacteria was investigated by colony forming unit (CFU) and disc diffusion methods.
34856218	1	33	theme	chitosan	205:212	arg1	CNPs					229:232	CNPs	229:232	CNPs	229:232	This study aimed to develop a plasticized starch (PS) based film loaded with chitosan nanoparticles (CNPs, 1, 2, 3, and 4%) as a reinforcing and antibacterial agent.
34856218	1	33	theme	chitosan	205:212	arg1	nanoparticles					214:226	chitosan nanoparticles	205:226	chitosan nanoparticles (CNPs, 1, 2, 3, and 4%) as a reinforcing and antibacterial agent	205:291	This study aimed to develop a plasticized starch (PS) based film loaded with chitosan nanoparticles (CNPs, 1, 2, 3, and 4%) as a reinforcing and antibacterial agent.
34856218	0	34	theme	mechanical	28:37	arg1	attributes					57:66	Biodegradability, physical, mechanical and antimicrobial attributes	0:66	Biodegradability, physical, mechanical and antimicrobial attributes of starch nanocomposites	0:91	Biodegradability, physical, mechanical and antimicrobial attributes of starch nanocomposites containing chitosan nanoparticles.
34856218	5	35	theme	improved	766:773	arg1	behaviour					786:794	improved mechanical behaviour	766:794	improved mechanical behaviour	766:794	The increase in the CNPs percentage led to improved mechanical behaviour and barrier properties.
34856218	2	36	theme	films	409:413	arg1	thermo-mechanical					352:368	thermo-mechanical	352:368	thermo-mechanical	352:368	We examined the morphology, biodegradability, mechanical, thermo-mechanical, and barrier properties of the PS/CNPs films.
34856218	2	36	theme	films	409:413	arg1	biodegradability					322:337	biodegradability	322:337	biodegradability	322:337	We examined the morphology, biodegradability, mechanical, thermo-mechanical, and barrier properties of the PS/CNPs films.
34856218	2	36	theme	films	409:413	arg1	properties					383:392	barrier properties	375:392	barrier properties	375:392	We examined the morphology, biodegradability, mechanical, thermo-mechanical, and barrier properties of the PS/CNPs films.
34856218	2	36	theme	films	409:413	arg1	morphology					310:319	morphology	310:319	morphology	310:319	We examined the morphology, biodegradability, mechanical, thermo-mechanical, and barrier properties of the PS/CNPs films.
34856218	1	37	dep	CNPs	229:232	arg1	%					249:249	1, 2, 3, and 4%	235:249	1, 2, 3, and 4%	235:249	This study aimed to develop a plasticized starch (PS) based film loaded with chitosan nanoparticles (CNPs, 1, 2, 3, and 4%) as a reinforcing and antibacterial agent.
34856218	4	38	theme	PS/CNPs	632:638	arg1	films					640:644	all PS/CNPs films	628:644	all PS/CNPs films	628:644	A dense structure was obtained for all PS/CNPs films and, thus, their complete biodegradation occurred in more days than neat PS.
34856218	0	39	theme	antimicrobial	43:55	arg1	attributes					57:66	Biodegradability, physical, mechanical and antimicrobial attributes	0:66	Biodegradability, physical, mechanical and antimicrobial attributes of starch nanocomposites	0:91	Biodegradability, physical, mechanical and antimicrobial attributes of starch nanocomposites containing chitosan nanoparticles.
34856218	6	40	theme	inhibition	853:862	arg1	zones					864:868	inhibition zones	853:868	inhibition zones	853:868	PS-CNPs composite films revealed inhibition zones against both E. coli and S. aureus, with the 100% reduction in CFU against S. aureus.
34856218	1	41	theme	reinforcing	257:267	arg1	agent					287:291	a reinforcing and antibacterial agent	255:291	a reinforcing and antibacterial agent	255:291	This study aimed to develop a plasticized starch (PS) based film loaded with chitosan nanoparticles (CNPs, 1, 2, 3, and 4%) as a reinforcing and antibacterial agent.
34856218	3	42	theme	antimicrobial	420:432	arg1	activity					434:441	The antimicrobial activity	416:441	The antimicrobial activity against both Gram-positive (S. aureus) and Gram-negative (E. coli) bacteria	416:517	The antimicrobial activity against both Gram-positive (S. aureus) and Gram-negative (E. coli) bacteria was investigated by colony forming unit (CFU) and disc diffusion methods.
34856218	4	43	theme	more	699:702	arg1	days					704:707	more days	699:707	more days than neat PS	699:720	A dense structure was obtained for all PS/CNPs films and, thus, their complete biodegradation occurred in more days than neat PS.
34856218	6	44	theme	%	918:918	arg1	reduction					920:928	the 100% reduction	911:928	the 100% reduction in CFU against S. aureus	911:953	PS-CNPs composite films revealed inhibition zones against both E. coli and S. aureus, with the 100% reduction in CFU against S. aureus.
34856218	0	45	theme	starch	71:76	arg1	nanocomposites					78:91	starch nanocomposites	71:91	starch nanocomposites	71:91	Biodegradability, physical, mechanical and antimicrobial attributes of starch nanocomposites containing chitosan nanoparticles.
34856218	4	46	theme	neat	714:717	arg1	PS					719:720	neat PS	714:720	neat PS	714:720	A dense structure was obtained for all PS/CNPs films and, thus, their complete biodegradation occurred in more days than neat PS.
34856218	6	47	theme	100	915:917	arg1	%					918:918	%	918:918	%	918:918	PS-CNPs composite films revealed inhibition zones against both E. coli and S. aureus, with the 100% reduction in CFU against S. aureus.
34856218	0	48	theme	physical	18:25	arg1	attributes					57:66	Biodegradability, physical, mechanical and antimicrobial attributes	0:66	Biodegradability, physical, mechanical and antimicrobial attributes of starch nanocomposites	0:91	Biodegradability, physical, mechanical and antimicrobial attributes of starch nanocomposites containing chitosan nanoparticles.
34856218	1	49	theme	plasticized	158:168	arg1	film					188:191	a plasticized starch (PS) based film	156:191	a plasticized starch (PS) based film loaded with chitosan nanoparticles (CNPs, 1, 2, 3, and 4%) as a reinforcing and antibacterial agent	156:291	This study aimed to develop a plasticized starch (PS) based film loaded with chitosan nanoparticles (CNPs, 1, 2, 3, and 4%) as a reinforcing and antibacterial agent.
34856218	7	50	theme	antimicrobial	1128:1140	arg1	packaging					1147:1155	antimicrobial food packaging	1128:1155	antimicrobial food packaging	1128:1155	The current study exhibited that PS-CNPs films were more effective in inhibiting bacteria growth than neat PS film, confirming the composite films potential application as antimicrobial food packaging.
34856218	7	51	theme	composite	1087:1095	arg1	application					1113:1123	the composite films potential application	1083:1123	the composite films potential application	1083:1123	The current study exhibited that PS-CNPs films were more effective in inhibiting bacteria growth than neat PS film, confirming the composite films potential application as antimicrobial food packaging.
33518299	6	0	theme	dismutase	1137:1145	arg1	activity					1147:1154	superoxide dismutase activity	1126:1154	superoxide dismutase activity in the jejunum (P < 0.05)	1126:1180	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	6	1	theme	jejunal	1283:1289	arg1	mucosa					1291:1296	the jejunal mucosa	1279:1296	the jejunal mucosa (P < 0.05)	1279:1307	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	6	1	theme	jejunal	1283:1289	arg1	P < 0.05					1299:1306	P < 0.05	1299:1306	P < 0.05	1299:1306	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	1	2	theme	broiler	260:266	arg1	breeders					268:275	broiler breeders	260:275	broiler breeders	260:275	This study was conducted to explore whether dietary pectic oligosaccharide (POS) supplementation could improve gut health of broiler breeders with different egg-laying rates.
33518299	8	3	theme	egg-laying	2074:2083	arg1	rates					2085:2089	different egg-laying rates	2064:2089	different egg-laying rates	2064:2089	These results suggest that supplementing POS in diets may elevate gut health via improvement of intestinal barrier function, antioxidant capacity, and microbiota composition in broiler breeders with different egg-laying rates.
33518299	6	4	theme	Dietary	1036:1042	arg1	supplementation					1048:1062	Dietary POS supplementation	1036:1062	Dietary POS supplementation	1036:1062	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	0	5	with	health	75:80	arg1	rates					128:132	different egg-laying rates	107:132	different egg-laying rates	107:132	The effect of dietary pectic oligosaccharide supplementation on intestinal health of broiler breeders with different egg-laying rates.
33518299	7	6	dep	phylum	1836:1841	arg1	the					1832:1834	the	1832:1834	the	1832:1834	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	6	7	theme	jejunal	1363:1369	arg1	P < 0.05					1379:1386	P < 0.05	1379:1386	P < 0.05	1379:1386	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	6	7	theme	jejunal	1363:1369	arg1	mucosa					1371:1376	the jejunal mucosa	1359:1376	the jejunal mucosa (P < 0.05)	1359:1387	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	5	8	theme	qualified	727:735	arg1	P < 0.05					747:754	P < 0.05	747:754	P < 0.05	747:754	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	8	theme	qualified	727:735	arg1	rate					741:744	qualified egg rate	727:744	qualified egg rate (P < 0.05)	727:755	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	1	9	theme	dietary	179:185	arg1	supplementation					216:230	dietary pectic oligosaccharide (POS) supplementation	179:230	dietary pectic oligosaccharide (POS) supplementation	179:230	This study was conducted to explore whether dietary pectic oligosaccharide (POS) supplementation could improve gut health of broiler breeders with different egg-laying rates.
33518299	1	10	theme	different	282:290	arg1	rates					303:307	different egg-laying rates	282:307	different egg-laying rates	282:307	This study was conducted to explore whether dietary pectic oligosaccharide (POS) supplementation could improve gut health of broiler breeders with different egg-laying rates.
33518299	2	11	theme	factorial	318:326	arg1	design					328:333	A 2 × 2 factorial design	310:333	A 2 × 2 factorial design	310:333	A 2 × 2 factorial design was used in this study.
33518299	6	12	theme	mRNA	1339:1342	arg1	expressions					1344:1354	TNF-α mRNA expressions	1333:1354	TNF-α mRNA expressions	1333:1354	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	1	13	theme	oligosaccharide	194:208	arg1	supplementation					216:230	dietary pectic oligosaccharide (POS) supplementation	179:230	dietary pectic oligosaccharide (POS) supplementation	179:230	This study was conducted to explore whether dietary pectic oligosaccharide (POS) supplementation could improve gut health of broiler breeders with different egg-laying rates.
33518299	7	14	from	jejunum	1680:1686	arg1	mucosa					1750:1755	the jejunal mucosa	1738:1755	the jejunal mucosa	1738:1755	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	7	15	theme	villus	1553:1558	arg1	height					1560:1565	villus height	1553:1565	villus height (P = 0.08)	1553:1576	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	7	15	theme	villus	1553:1558	arg1	P = 0.08					1568:1575	P = 0.08	1568:1575	P = 0.08	1568:1575	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	5	16	theme	rate	656:659	arg1	breeders					669:676	average egg-laying rate broiler breeders	637:676	average egg-laying rate broiler breeders	637:676	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	17	theme	egg	765:767	arg1	weight					769:774	higher egg weight	758:774	higher egg weight	758:774	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	6	18	theme	breeders	1096:1103	arg1	weight					1078:1083	egg weight	1074:1083	egg weight of broiler breeders (P < 0.05)	1074:1114	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	6	19	theme	mRNA	1260:1263	arg1	expression					1265:1274	zonula occluden 1 mRNA expression	1242:1274	zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05)	1242:1307	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	6	20	theme	relative	1404:1411	arg1	abundance					1413:1421	relative abundance	1404:1421	relative abundance of some microbiota (including the phylum and genus, P < 0.05)	1404:1483	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	7	21	theme	TNF-α	1712:1716	arg1	expressions					1723:1733	TNF-α mRNA expressions	1712:1733	TNF-α mRNA expressions	1712:1733	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	8	22	theme	intestinal	1961:1970	arg1	function					1980:1987	intestinal barrier function	1961:1987	intestinal barrier function	1961:1987	These results suggest that supplementing POS in diets may elevate gut health via improvement of intestinal barrier function, antioxidant capacity, and microbiota composition in broiler breeders with different egg-laying rates.
33518299	6	23	dep	supplementation	1048:1062	arg1	POS					1044:1046	Dietary POS supplementation	1036:1062	Dietary POS supplementation	1036:1062	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	5	24	theme	factor	919:924	arg1	expressions					952:962	higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions	877:962	higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa	877:984	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	8	25	theme	function	1980:1987	arg1	improvement					1946:1956	improvement	1946:1956	improvement of intestinal barrier function, antioxidant capacity, and microbiota composition in broiler breeders with different egg-laying rates	1946:2089	These results suggest that supplementing POS in diets may elevate gut health via improvement of intestinal barrier function, antioxidant capacity, and microbiota composition in broiler breeders with different egg-laying rates.
33518299	6	26	theme	egg	1074:1076	arg1	weight					1078:1083	egg weight	1074:1083	egg weight of broiler breeders (P < 0.05)	1074:1114	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	5	27	theme	average	637:643	arg1	breeders					669:676	average egg-laying rate broiler breeders	637:676	average egg-laying rate broiler breeders	637:676	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	28	from	ratio	796:800	arg1	mucosa					979:984	the jejunal mucosa	967:984	the jejunal mucosa	967:984	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	28	from	ratio	796:800	arg1	jejunum					857:863	the jejunum	853:863	the jejunum (P < 0.05)	853:874	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	28	from	ratio	796:800	arg1	P < 0.05					866:873	P < 0.05	866:873	P < 0.05	866:873	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	28	from	ratio	796:800	arg1	digesta					1027:1033	cecal digesta	1021:1033	cecal digesta	1021:1033	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	8	29	from	POS	1906:1908	arg1	diets					1913:1917	diets	1913:1917	diets	1913:1917	These results suggest that supplementing POS in diets may elevate gut health via improvement of intestinal barrier function, antioxidant capacity, and microbiota composition in broiler breeders with different egg-laying rates.
33518299	8	30	theme	antioxidant	1990:2000	arg1	capacity					2002:2009	antioxidant capacity	1990:2009	antioxidant capacity	1990:2009	These results suggest that supplementing POS in diets may elevate gut health via improvement of intestinal barrier function, antioxidant capacity, and microbiota composition in broiler breeders with different egg-laying rates.
33518299	5	31	theme	lower	705:709	arg1	rate					718:721	lower laying rate	705:721	lower laying rate	705:721	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	32	theme	jejunal	971:977	arg1	mucosa					979:984	the jejunal mucosa	967:984	the jejunal mucosa	967:984	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	7	33	theme	LELR	1502:1505	arg1	breeders					1515:1522	LELR broiler breeders	1502:1522	LELR broiler breeders	1502:1522	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	5	34	theme	higher	814:819	arg1	MDA					838:840	MDA	838:840	MDA	838:840	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	34	theme	higher	814:819	arg1	malondialdehyde					821:835	higher malondialdehyde	814:835	higher malondialdehyde (MDA) levels in the jejunum (P < 0.05)	814:874	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	35	contain	had	701:703	arg2	levels					843:848	higher malondialdehyde (MDA) levels	814:848	higher malondialdehyde (MDA) levels in the jejunum (P < 0.05)	814:874	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	35	contain	had	701:703	arg2	diversity					1008:1016	lower microflora diversity	991:1016	lower microflora diversity in cecal digesta	991:1033	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	35	contain	had	701:703	arg2	rate					741:744	qualified egg rate	727:744	qualified egg rate (P < 0.05)	727:755	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	35	contain	had	701:703	arg2	rate					718:721	lower laying rate	705:721	lower laying rate	705:721	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	35	contain	had	701:703	arg2	P < 0.05					747:754	P < 0.05	747:754	P < 0.05	747:754	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	35	contain	had	701:703	arg1	breeders					692:699	LELR broiler breeders	679:699	LELR broiler breeders	679:699	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	35	contain	had	701:703	arg2	expressions					952:962	higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions	877:962	higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa	877:984	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	35	contain	had	701:703	arg2	weight					769:774	higher egg weight	758:774	higher egg weight	758:774	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	0	36	theme	breeders	93:100	arg1	health					75:80	intestinal health	64:80	intestinal health of broiler breeders with different egg-laying rates	64:132	The effect of dietary pectic oligosaccharide supplementation on intestinal health of broiler breeders with different egg-laying rates.
33518299	5	37	theme	mRNA	947:950	arg1	expressions					952:962	higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions	877:962	higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa	877:984	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	6	38	from	expressions	1344:1354	arg1	P < 0.05					1379:1386	P < 0.05	1379:1386	P < 0.05	1379:1386	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	6	38	from	expressions	1344:1354	arg1	mucosa					1371:1376	the jejunal mucosa	1359:1376	the jejunal mucosa (P < 0.05)	1359:1387	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	3	39	dep	breeders	401:408	arg1	48 wk					411:415	48 wk	411:415	48 wk of age	411:422	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	7	40	dep	administration	1529:1542	arg1	POS					1525:1527	POS administration	1525:1542	POS administration	1525:1542	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	0	41	theme	different	107:115	arg1	rates					128:132	different egg-laying rates	107:132	different egg-laying rates	107:132	The effect of dietary pectic oligosaccharide supplementation on intestinal health of broiler breeders with different egg-laying rates.
33518299	0	42	theme	oligosaccharide	29:43	arg1	supplementation					45:59	dietary pectic oligosaccharide supplementation	14:59	dietary pectic oligosaccharide supplementation	14:59	The effect of dietary pectic oligosaccharide supplementation on intestinal health of broiler breeders with different egg-laying rates.
33518299	6	43	theme	microbiota	1431:1440	arg1	abundance					1413:1421	relative abundance	1404:1421	relative abundance of some microbiota (including the phylum and genus, P < 0.05)	1404:1483	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	8	44	from	improvement	1946:1956	arg1	breeders					2050:2057	broiler breeders	2042:2057	broiler breeders with different egg-laying rates	2042:2089	These results suggest that supplementing POS in diets may elevate gut health via improvement of intestinal barrier function, antioxidant capacity, and microbiota composition in broiler breeders with different egg-laying rates.
33518299	3	45	theme	Acres	387:391	arg1	birds					499:503	128 low egg-laying rate (LELR) birds	468:503	128 low egg-laying rate (LELR) birds	468:503	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	3	45	theme	Acres	387:391	arg1	breeders					401:408	Two hundred fifty-six Arbor Acres broiler breeders	359:408	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age)	359:423	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	3	45	theme	Acres	387:391	arg1	rate					459:462	128 average egg-laying rate	436:462	128 average egg-laying rate	436:462	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	2	46	used	used	339:342	arg2	design					328:333	A 2 × 2 factorial design	310:333	A 2 × 2 factorial design	310:333	A 2 × 2 factorial design was used in this study.
33518299	3	47	theme	age	420:422	arg1	48 wk					411:415	48 wk	411:415	48 wk of age	411:422	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	5	48	theme	cecal	1021:1025	arg1	digesta					1027:1033	cecal digesta	1021:1033	cecal digesta	1021:1033	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	6	49	from	IL-6	1324:1327	arg1	P < 0.05					1379:1386	P < 0.05	1379:1386	P < 0.05	1379:1386	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	6	49	from	IL-6	1324:1327	arg1	mucosa					1371:1376	the jejunal mucosa	1359:1376	the jejunal mucosa (P < 0.05)	1359:1387	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	3	50	theme	fifty-six	371:379	arg1	birds					499:503	128 low egg-laying rate (LELR) birds	468:503	128 low egg-laying rate (LELR) birds	468:503	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	3	50	theme	fifty-six	371:379	arg1	breeders					401:408	Two hundred fifty-six Arbor Acres broiler breeders	359:408	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age)	359:423	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	3	50	theme	fifty-six	371:379	arg1	rate					459:462	128 average egg-laying rate	436:462	128 average egg-laying rate	436:462	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	3	51	theme	egg-laying	448:457	arg1	rate					459:462	128 average egg-laying rate	436:462	128 average egg-laying rate	436:462	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	5	52	from	expressions	952:962	arg1	mucosa					979:984	the jejunal mucosa	967:984	the jejunal mucosa	967:984	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	52	from	expressions	952:962	arg1	jejunum					857:863	the jejunum	853:863	the jejunum (P < 0.05)	853:874	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	52	from	expressions	952:962	arg1	P < 0.05					866:873	P < 0.05	866:873	P < 0.05	866:873	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	52	from	expressions	952:962	arg1	digesta					1027:1033	cecal digesta	1021:1033	cecal digesta	1021:1033	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	6	53	dep	phylum	1457:1462	arg1	the					1453:1455	the	1453:1455	the	1453:1455	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	6	54	theme	zonula	1242:1247	arg1	expression					1265:1274	zonula occluden 1 mRNA expression	1242:1274	zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05)	1242:1307	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	5	55	theme	lower	991:995	arg1	diversity					1008:1016	lower microflora diversity	991:1016	lower microflora diversity in cecal digesta	991:1033	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	0	56	theme	pectic	22:27	arg1	supplementation					45:59	dietary pectic oligosaccharide supplementation	14:59	dietary pectic oligosaccharide supplementation	14:59	The effect of dietary pectic oligosaccharide supplementation on intestinal health of broiler breeders with different egg-laying rates.
33518299	8	57	theme	microbiota	2016:2025	arg1	composition					2027:2037	microbiota composition	2016:2037	microbiota composition	2016:2037	These results suggest that supplementing POS in diets may elevate gut health via improvement of intestinal barrier function, antioxidant capacity, and microbiota composition in broiler breeders with different egg-laying rates.
33518299	0	58	theme	intestinal	64:73	arg1	health					75:80	intestinal health	64:80	intestinal health of broiler breeders with different egg-laying rates	64:132	The effect of dietary pectic oligosaccharide supplementation on intestinal health of broiler breeders with different egg-laying rates.
33518299	3	59	theme	low	472:474	arg1	birds					499:503	128 low egg-laying rate (LELR) birds	468:503	128 low egg-laying rate (LELR) birds	468:503	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	0	60	theme	supplementation	45:59	arg1	effect					4:9	The effect	0:9	The effect of dietary pectic oligosaccharide supplementation on intestinal health of broiler breeders with different egg-laying rates.	0:133	The effect of dietary pectic oligosaccharide supplementation on intestinal health of broiler breeders with different egg-laying rates.
33518299	5	61	theme	necrosis	910:917	arg1	factor					919:924	tumor necrosis factor α	904:926	tumor necrosis factor α (TNF-α)	904:934	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	61	theme	necrosis	910:917	arg1	TNF-α					929:933	TNF-α	929:933	TNF-α	929:933	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	62	theme	IL-6	884:887	arg1	expressions					952:962	higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions	877:962	higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa	877:984	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	3	63	theme	average	440:446	arg1	rate					459:462	128 average egg-laying rate	436:462	128 average egg-laying rate	436:462	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	3	64	theme	LELR	493:496	arg1	birds					499:503	128 low egg-laying rate (LELR) birds	468:503	128 low egg-laying rate (LELR) birds	468:503	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	5	65	theme	conversion	785:794	arg1	P < 0.05					803:810	P < 0.05	803:810	P < 0.05	803:810	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	65	theme	conversion	785:794	arg1	ratio					796:800	feed conversion ratio	780:800	feed conversion ratio (P < 0.05)	780:811	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	1	66	theme	gut	246:248	arg1	health					250:255	gut health	246:255	gut health of broiler breeders with different egg-laying rates	246:307	This study was conducted to explore whether dietary pectic oligosaccharide (POS) supplementation could improve gut health of broiler breeders with different egg-laying rates.
33518299	8	67	theme	different	2064:2072	arg1	rates					2085:2089	different egg-laying rates	2064:2089	different egg-laying rates	2064:2089	These results suggest that supplementing POS in diets may elevate gut health via improvement of intestinal barrier function, antioxidant capacity, and microbiota composition in broiler breeders with different egg-laying rates.
33518299	7	68	theme	mRNA	1587:1590	arg1	expression					1592:1601	mRNA expression	1587:1601	mRNA expression	1587:1601	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	7	69	dep	enhanced	1544:1551	arg1	P = 0.09					1604:1611	P = 0.09	1604:1611	P = 0.09	1604:1611	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	5	70	theme	laying	711:716	arg1	rate					718:721	lower laying rate	705:721	lower laying rate	705:721	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	71	from	diversity	1008:1016	arg1	mucosa					979:984	the jejunal mucosa	967:984	the jejunal mucosa	967:984	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	71	from	diversity	1008:1016	arg1	jejunum					857:863	the jejunum	853:863	the jejunum (P < 0.05)	853:874	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	71	from	diversity	1008:1016	arg1	P < 0.05					866:873	P < 0.05	866:873	P < 0.05	866:873	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	71	from	diversity	1008:1016	arg1	digesta					1027:1033	cecal digesta	1021:1033	cecal digesta	1021:1033	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	3	72	theme	rate	487:490	arg1	birds					499:503	128 low egg-laying rate (LELR) birds	468:503	128 low egg-laying rate (LELR) birds	468:503	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	7	73	theme	relative	1783:1790	arg1	abundance					1792:1800	relative abundance	1783:1800	relative abundance of some microbiota (including the phylum and genus, P < 0.05)	1783:1862	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	7	74	from	expressions	1723:1733	arg1	mucosa					1750:1755	the jejunal mucosa	1738:1755	the jejunal mucosa	1738:1755	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	7	75	dep	height	1560:1565	arg1	expression					1592:1601	mRNA expression	1587:1601	mRNA expression	1587:1601	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	6	76	theme	superoxide	1126:1135	arg1	activity					1147:1154	superoxide dismutase activity	1126:1154	superoxide dismutase activity in the jejunum (P < 0.05)	1126:1180	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	1	77	theme	breeders	268:275	arg1	health					250:255	gut health	246:255	gut health of broiler breeders with different egg-laying rates	246:307	This study was conducted to explore whether dietary pectic oligosaccharide (POS) supplementation could improve gut health of broiler breeders with different egg-laying rates.
33518299	7	78	theme	broiler	1507:1513	arg1	breeders					1515:1522	LELR broiler breeders	1502:1522	LELR broiler breeders	1502:1522	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	5	79	theme	feed	780:783	arg1	P < 0.05					803:810	P < 0.05	803:810	P < 0.05	803:810	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	79	theme	feed	780:783	arg1	ratio					796:800	feed conversion ratio	780:800	feed conversion ratio (P < 0.05)	780:811	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	1	80	theme	pectic	187:192	arg1	supplementation					216:230	dietary pectic oligosaccharide (POS) supplementation	179:230	dietary pectic oligosaccharide (POS) supplementation	179:230	This study was conducted to explore whether dietary pectic oligosaccharide (POS) supplementation could improve gut health of broiler breeders with different egg-laying rates.
33518299	5	81	theme	egg-laying	645:654	arg1	breeders					669:676	average egg-laying rate broiler breeders	637:676	average egg-laying rate broiler breeders	637:676	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	2	82	theme	2 × 2	312:316	arg1	design					328:333	A 2 × 2 factorial design	310:333	A 2 × 2 factorial design	310:333	A 2 × 2 factorial design was used in this study.
33518299	6	83	theme	TNF-α	1333:1337	arg1	expressions					1344:1354	TNF-α mRNA expressions	1333:1354	TNF-α mRNA expressions	1333:1354	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	5	84	theme	higher	758:763	arg1	weight					769:774	higher egg weight	758:774	higher egg weight	758:774	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	7	85	theme	jejunal	1742:1748	arg1	mucosa					1750:1755	the jejunal mucosa	1738:1755	the jejunal mucosa	1738:1755	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	8	86	theme	gut	1931:1933	arg1	health					1935:1940	gut health	1931:1940	gut health	1931:1940	These results suggest that supplementing POS in diets may elevate gut health via improvement of intestinal barrier function, antioxidant capacity, and microbiota composition in broiler breeders with different egg-laying rates.
33518299	5	87	theme	broiler	661:667	arg1	breeders					669:676	average egg-laying rate broiler breeders	637:676	average egg-laying rate broiler breeders	637:676	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	6	88	theme	broiler	1088:1094	arg1	P < 0.05					1106:1113	P < 0.05	1106:1113	P < 0.05	1106:1113	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	6	88	theme	broiler	1088:1094	arg1	breeders					1096:1103	broiler breeders	1088:1103	broiler breeders (P < 0.05)	1088:1114	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	6	89	from	expression	1265:1274	arg1	mucosa					1291:1296	the jejunal mucosa	1279:1296	the jejunal mucosa (P < 0.05)	1279:1307	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	6	89	from	expression	1265:1274	arg1	P < 0.05					1299:1306	P < 0.05	1299:1306	P < 0.05	1299:1306	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	5	90	theme	malondialdehyde	821:835	arg1	levels					843:848	higher malondialdehyde (MDA) levels	814:848	higher malondialdehyde (MDA) levels in the jejunum (P < 0.05)	814:874	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	7	91	theme	mRNA	1718:1721	arg1	expressions					1723:1733	TNF-α mRNA expressions	1712:1733	TNF-α mRNA expressions	1712:1733	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	7	92	from	IL-6	1703:1706	arg1	mucosa					1750:1755	the jejunal mucosa	1738:1755	the jejunal mucosa	1738:1755	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	7	93	theme	MDA	1663:1665	arg1	level					1667:1671	the increasing MDA level	1648:1671	the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05)	1648:1766	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	5	94	from	rate	718:721	arg1	mucosa					979:984	the jejunal mucosa	967:984	the jejunal mucosa	967:984	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	94	from	rate	718:721	arg1	jejunum					857:863	the jejunum	853:863	the jejunum (P < 0.05)	853:874	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	94	from	rate	718:721	arg1	P < 0.05					866:873	P < 0.05	866:873	P < 0.05	866:873	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	94	from	rate	718:721	arg1	digesta					1027:1033	cecal digesta	1021:1033	cecal digesta	1021:1033	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	3	95	theme	POS	583:585	arg1	200 mg kg-1					568:578	200 mg kg-1	568:578	200 mg kg-1 of POS (n = 8)	568:593	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	8	96	theme	barrier	1972:1978	arg1	function					1980:1987	intestinal barrier function	1961:1987	intestinal barrier function	1961:1987	These results suggest that supplementing POS in diets may elevate gut health via improvement of intestinal barrier function, antioxidant capacity, and microbiota composition in broiler breeders with different egg-laying rates.
33518299	8	97	with	breeders	2050:2057	arg1	rates					2085:2089	different egg-laying rates	2064:2089	different egg-laying rates	2064:2089	These results suggest that supplementing POS in diets may elevate gut health via improvement of intestinal barrier function, antioxidant capacity, and microbiota composition in broiler breeders with different egg-laying rates.
33518299	5	98	theme	LELR	679:682	arg1	breeders					692:699	LELR broiler breeders	679:699	LELR broiler breeders	679:699	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	0	99	theme	broiler	85:91	arg1	breeders					93:100	broiler breeders	85:100	broiler breeders	85:100	The effect of dietary pectic oligosaccharide supplementation on intestinal health of broiler breeders with different egg-laying rates.
33518299	5	100	from	rate	741:744	arg1	mucosa					979:984	the jejunal mucosa	967:984	the jejunal mucosa	967:984	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	100	from	rate	741:744	arg1	jejunum					857:863	the jejunum	853:863	the jejunum (P < 0.05)	853:874	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	100	from	rate	741:744	arg1	P < 0.05					866:873	P < 0.05	866:873	P < 0.05	866:873	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	100	from	rate	741:744	arg1	digesta					1027:1033	cecal digesta	1021:1033	cecal digesta	1021:1033	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	6	101	from	level	1197:1201	arg1	P < 0.05					1219:1226	P < 0.05	1219:1226	P < 0.05	1219:1226	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	6	101	from	level	1197:1201	arg1	jejunum					1210:1216	the jejunum	1206:1216	the jejunum (P < 0.05)	1206:1227	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	0	102	from	effect	4:9	arg1	health					75:80	intestinal health	64:80	intestinal health of broiler breeders with different egg-laying rates	64:132	The effect of dietary pectic oligosaccharide supplementation on intestinal health of broiler breeders with different egg-laying rates.
33518299	7	103	theme	microbiota	1810:1819	arg1	abundance					1792:1800	relative abundance	1783:1800	relative abundance of some microbiota (including the phylum and genus, P < 0.05)	1783:1862	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	1	104	dep	supplementation	216:230	arg1	POS					211:213	dietary pectic oligosaccharide (POS) supplementation	179:230	dietary pectic oligosaccharide (POS) supplementation	179:230	This study was conducted to explore whether dietary pectic oligosaccharide (POS) supplementation could improve gut health of broiler breeders with different egg-laying rates.
33518299	7	105	from	level	1667:1671	arg1	P < 0.05					1758:1765	P < 0.05	1758:1765	P < 0.05	1758:1765	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	7	105	from	level	1667:1671	arg1	P < 0.05					1689:1696	P < 0.05	1689:1696	P < 0.05	1689:1696	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	7	105	from	level	1667:1671	arg1	IL-6					1703:1706	IL-6	1703:1706	IL-6	1703:1706	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	7	105	from	level	1667:1671	arg1	jejunum					1680:1686	the jejunum	1676:1686	the jejunum (P < 0.05)	1676:1697	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	0	106	theme	egg-laying	117:126	arg1	rates					128:132	different egg-laying rates	107:132	different egg-laying rates	107:132	The effect of dietary pectic oligosaccharide supplementation on intestinal health of broiler breeders with different egg-laying rates.
33518299	5	107	theme	broiler	684:690	arg1	breeders					692:699	LELR broiler breeders	679:699	LELR broiler breeders	679:699	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	3	108	theme	broiler	393:399	arg1	birds					499:503	128 low egg-laying rate (LELR) birds	468:503	128 low egg-laying rate (LELR) birds	468:503	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	3	108	theme	broiler	393:399	arg1	breeders					401:408	Two hundred fifty-six Arbor Acres broiler breeders	359:408	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age)	359:423	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	3	108	theme	broiler	393:399	arg1	rate					459:462	128 average egg-laying rate	436:462	128 average egg-laying rate	436:462	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	0	109	theme	dietary	14:20	arg1	supplementation					45:59	dietary pectic oligosaccharide supplementation	14:59	dietary pectic oligosaccharide supplementation	14:59	The effect of dietary pectic oligosaccharide supplementation on intestinal health of broiler breeders with different egg-laying rates.
33518299	1	110	theme	egg-laying	292:301	arg1	rates					303:307	different egg-laying rates	282:307	different egg-laying rates	282:307	This study was conducted to explore whether dietary pectic oligosaccharide (POS) supplementation could improve gut health of broiler breeders with different egg-laying rates.
33518299	3	111	theme	Arbor	381:385	arg1	birds					499:503	128 low egg-laying rate (LELR) birds	468:503	128 low egg-laying rate (LELR) birds	468:503	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	3	111	theme	Arbor	381:385	arg1	breeders					401:408	Two hundred fifty-six Arbor Acres broiler breeders	359:408	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age)	359:423	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	3	111	theme	Arbor	381:385	arg1	rate					459:462	128 average egg-laying rate	436:462	128 average egg-laying rate	436:462	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	6	112	theme	occluden	1249:1256	arg1	expression					1265:1274	zonula occluden 1 mRNA expression	1242:1274	zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05)	1242:1307	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	7	113	theme	increasing	1652:1661	arg1	level					1667:1671	the increasing MDA level	1648:1671	the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05)	1648:1766	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	8	114	theme	capacity	2002:2009	arg1	improvement					1946:1956	improvement	1946:1956	improvement of intestinal barrier function, antioxidant capacity, and microbiota composition in broiler breeders with different egg-laying rates	1946:2089	These results suggest that supplementing POS in diets may elevate gut health via improvement of intestinal barrier function, antioxidant capacity, and microbiota composition in broiler breeders with different egg-laying rates.
33518299	6	115	from	activity	1147:1154	arg1	jejunum					1163:1169	the jejunum	1159:1169	the jejunum (P < 0.05)	1159:1180	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	6	115	from	activity	1147:1154	arg1	P < 0.05					1172:1179	P < 0.05	1172:1179	P < 0.05	1172:1179	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	6	116	theme	MDA	1193:1195	arg1	level					1197:1201	MDA level	1193:1201	MDA level in the jejunum (P < 0.05)	1193:1227	Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	5	117	theme	egg	737:739	arg1	P < 0.05					747:754	P < 0.05	747:754	P < 0.05	747:754	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	117	theme	egg	737:739	arg1	rate					741:744	qualified egg rate	727:744	qualified egg rate (P < 0.05)	727:755	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	118	theme	microflora	997:1006	arg1	diversity					1008:1016	lower microflora diversity	991:1016	lower microflora diversity in cecal digesta	991:1033	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	119	theme	tumor	904:908	arg1	factor					919:924	tumor necrosis factor α	904:926	tumor necrosis factor α (TNF-α)	904:934	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	119	theme	tumor	904:908	arg1	TNF-α					929:933	TNF-α	929:933	TNF-α	929:933	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	7	120	theme	jejunal	1621:1627	arg1	mucosa					1629:1634	the jejunal mucosa	1617:1634	the jejunal mucosa	1617:1634	In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05).
33518299	3	121	theme	egg-laying	476:485	arg1	birds					499:503	128 low egg-laying rate (LELR) birds	468:503	128 low egg-laying rate (LELR) birds	468:503	Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg-1 of POS (n = 8).
33518299	8	122	theme	composition	2027:2037	arg1	improvement					1946:1956	improvement	1946:1956	improvement of intestinal barrier function, antioxidant capacity, and microbiota composition in broiler breeders with different egg-laying rates	1946:2089	These results suggest that supplementing POS in diets may elevate gut health via improvement of intestinal barrier function, antioxidant capacity, and microbiota composition in broiler breeders with different egg-laying rates.
33518299	5	123	theme	higher	877:882	arg1	P < 0.05					890:897	P < 0.05	890:897	P < 0.05	890:897	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	123	theme	higher	877:882	arg1	P = 0.07					937:944	P = 0.07	937:944	P = 0.07	937:944	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	123	theme	higher	877:882	arg1	IL-6					884:887	higher IL-6	877:887	higher IL-6 (P < 0.05)	877:898	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	124	from	weight	769:774	arg1	mucosa					979:984	the jejunal mucosa	967:984	the jejunal mucosa	967:984	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	124	from	weight	769:774	arg1	jejunum					857:863	the jejunum	853:863	the jejunum (P < 0.05)	853:874	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	124	from	weight	769:774	arg1	P < 0.05					866:873	P < 0.05	866:873	P < 0.05	866:873	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	124	from	weight	769:774	arg1	digesta					1027:1033	cecal digesta	1021:1033	cecal digesta	1021:1033	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	125	from	levels	843:848	arg1	mucosa					979:984	the jejunal mucosa	967:984	the jejunal mucosa	967:984	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	125	from	levels	843:848	arg1	jejunum					857:863	the jejunum	853:863	the jejunum (P < 0.05)	853:874	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	125	from	levels	843:848	arg1	P < 0.05					866:873	P < 0.05	866:873	P < 0.05	866:873	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	5	125	from	levels	843:848	arg1	digesta					1027:1033	cecal digesta	1021:1033	cecal digesta	1021:1033	Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta.
33518299	8	126	theme	broiler	2042:2048	arg1	breeders					2050:2057	broiler breeders	2042:2057	broiler breeders with different egg-laying rates	2042:2089	These results suggest that supplementing POS in diets may elevate gut health via improvement of intestinal barrier function, antioxidant capacity, and microbiota composition in broiler breeders with different egg-laying rates.
33518299	1	127	with	health	250:255	arg1	rates					303:307	different egg-laying rates	282:307	different egg-laying rates	282:307	This study was conducted to explore whether dietary pectic oligosaccharide (POS) supplementation could improve gut health of broiler breeders with different egg-laying rates.
34297017	8	0	theme	stem	1184:1187	arg1	cells					1189:1193	hair follicle stem cells	1170:1193	hair follicle stem cells	1170:1193	In addition, the PDGF-BB/SA/Dex hydrogels promoted the EC-differentiation of transplanted BMSCs and proliferation of hair follicle stem cells in the wound.
34297017	8	1	theme	hair	1170:1173	arg1	cells					1189:1193	hair follicle stem cells	1170:1193	hair follicle stem cells	1170:1193	In addition, the PDGF-BB/SA/Dex hydrogels promoted the EC-differentiation of transplanted BMSCs and proliferation of hair follicle stem cells in the wound.
34297017	7	2	theme	wound	983:987	arg1	healing					989:995	wound healing	983:995	wound healing	983:995	Besides, these composite hydrogels loaded with BMSCs could accelerate wound healing by improving epithelialization and collagen deposition.
34297017	1	3	theme	interactive	181:191	arg1	healing					138:144	Wound healing	132:144	Wound healing	132:144	Wound healing is a well-orchestrated dynamic and interactive process, which needs a favorable microenvironment and suitable angiogenesis.
34297017	1	3	theme	interactive	181:191	arg1	process					193:199	a well-orchestrated dynamic and interactive process	149:199	a well-orchestrated dynamic and interactive process	149:199	Wound healing is a well-orchestrated dynamic and interactive process, which needs a favorable microenvironment and suitable angiogenesis.
34297017	10	4	theme	new	1593:1595	arg1	strategy					1609:1616	a new therapeutic strategy	1591:1616	a new therapeutic strategy for stem cell therapy in wound healing	1591:1655	In conclusion, the PDGF-BB/SA/Dex injectable hydrogels could accelerate BMSC-mediated skin wound healing by promoting angiogenesis via the activation of the PDGF-BB/PDGFR-β-mediated PI3K/Akt/eNOS pathway, which may provide a new therapeutic strategy for stem cell therapy in wound healing.
34297017	8	5	theme	follicle	1175:1182	arg1	cells					1189:1193	hair follicle stem cells	1170:1193	hair follicle stem cells	1170:1193	In addition, the PDGF-BB/SA/Dex hydrogels promoted the EC-differentiation of transplanted BMSCs and proliferation of hair follicle stem cells in the wound.
34297017	10	6	theme	BMSC-mediated	1440:1452	arg1	healing					1465:1471	BMSC-mediated skin wound healing	1440:1471	BMSC-mediated skin wound healing	1440:1471	In conclusion, the PDGF-BB/SA/Dex injectable hydrogels could accelerate BMSC-mediated skin wound healing by promoting angiogenesis via the activation of the PDGF-BB/PDGFR-β-mediated PI3K/Akt/eNOS pathway, which may provide a new therapeutic strategy for stem cell therapy in wound healing.
34297017	6	7	theme	excellent	864:872	arg1	biocompatibility					874:889	excellent biocompatibility	864:889	excellent biocompatibility	864:889	The PDGF-BB/SA/Dex hydrogels could sustainably release PDGF-BB with excellent biocompatibility in vitro and in vivo.
34297017	10	8	theme	wound	1643:1647	arg1	healing					1649:1655	wound healing	1643:1655	wound healing	1643:1655	In conclusion, the PDGF-BB/SA/Dex injectable hydrogels could accelerate BMSC-mediated skin wound healing by promoting angiogenesis via the activation of the PDGF-BB/PDGFR-β-mediated PI3K/Akt/eNOS pathway, which may provide a new therapeutic strategy for stem cell therapy in wound healing.
34297017	10	9	theme	stem	1622:1625	arg1	therapy					1632:1638	stem cell therapy	1622:1638	stem cell therapy in wound healing	1622:1655	In conclusion, the PDGF-BB/SA/Dex injectable hydrogels could accelerate BMSC-mediated skin wound healing by promoting angiogenesis via the activation of the PDGF-BB/PDGFR-β-mediated PI3K/Akt/eNOS pathway, which may provide a new therapeutic strategy for stem cell therapy in wound healing.
34297017	2	10	theme	crucial	322:328	arg1	role					330:333	a crucial role	320:333	a crucial role	320:333	Platelet derived growth factor-BB (PDGF-BB) plays a crucial role in wound healing.
34297017	3	11	theme	PDGF-BB	385:391	arg1	half-life					372:380	the short half-life	362:380	the short half-life of PDGF-BB	362:391	However, the short half-life of PDGF-BB limits its efficacy.
34297017	3	12	theme	short	366:370	arg1	half-life					372:380	the short half-life	362:380	the short half-life of PDGF-BB	362:391	However, the short half-life of PDGF-BB limits its efficacy.
34297017	4	13	theme	sodium	492:497	arg1	SA					509:510	SA	509:510	SA	509:510	In the present study, we successfully synthesized an injectable hydrogel with sodium alginate (SA) and dextran (Dex) as a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound.
34297017	4	13	theme	sodium	492:497	arg1	alginate					499:506	sodium alginate	492:506	sodium alginate (SA)	492:511	In the present study, we successfully synthesized an injectable hydrogel with sodium alginate (SA) and dextran (Dex) as a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound.
34297017	10	14	theme	PDGF-BB/PDGFR-β-mediated	1525:1548	arg1	pathway					1564:1570	the PDGF-BB/PDGFR-β-mediated PI3K/Akt/eNOS pathway	1521:1570	the PDGF-BB/PDGFR-β-mediated PI3K/Akt/eNOS pathway	1521:1570	In conclusion, the PDGF-BB/SA/Dex injectable hydrogels could accelerate BMSC-mediated skin wound healing by promoting angiogenesis via the activation of the PDGF-BB/PDGFR-β-mediated PI3K/Akt/eNOS pathway, which may provide a new therapeutic strategy for stem cell therapy in wound healing.
34297017	4	15	theme	present	421:427	arg1	study					429:433	the present study	417:433	the present study	417:433	In the present study, we successfully synthesized an injectable hydrogel with sodium alginate (SA) and dextran (Dex) as a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound.
34297017	6	16	with	PDGF-BB	851:857	arg1	biocompatibility					874:889	excellent biocompatibility	864:889	excellent biocompatibility	864:889	The PDGF-BB/SA/Dex hydrogels could sustainably release PDGF-BB with excellent biocompatibility in vitro and in vivo.
34297017	5	17	theme	BMSCs	780:784	arg1	migration					725:733	migration	725:733	migration	725:733	Our work demonstrates that the PDGF-BB protein enhanced the survival, migration and endothelial cell (EC) differentiation of BMSCs in vitro.
34297017	5	17	theme	BMSCs	780:784	arg1	survival					715:722	survival	715:722	survival	715:722	Our work demonstrates that the PDGF-BB protein enhanced the survival, migration and endothelial cell (EC) differentiation of BMSCs in vitro.
34297017	5	17	theme	BMSCs	780:784	arg1	differentiation					761:775	endothelial cell (EC) differentiation	739:775	endothelial cell (EC) differentiation	739:775	Our work demonstrates that the PDGF-BB protein enhanced the survival, migration and endothelial cell (EC) differentiation of BMSCs in vitro.
34297017	7	18	theme	composite	928:936	arg1	hydrogels					938:946	these composite hydrogels	922:946	these composite hydrogels loaded with BMSCs	922:964	Besides, these composite hydrogels loaded with BMSCs could accelerate wound healing by improving epithelialization and collagen deposition.
34297017	4	19	with	hydrogel	478:485	arg1	Dex					526:528	Dex	526:528	Dex	526:528	In the present study, we successfully synthesized an injectable hydrogel with sodium alginate (SA) and dextran (Dex) as a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound.
34297017	4	19	with	hydrogel	478:485	arg1	SA					509:510	SA	509:510	SA	509:510	In the present study, we successfully synthesized an injectable hydrogel with sodium alginate (SA) and dextran (Dex) as a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound.
34297017	4	19	with	hydrogel	478:485	arg1	dextran					517:523	dextran	517:523	dextran (Dex)	517:529	In the present study, we successfully synthesized an injectable hydrogel with sodium alginate (SA) and dextran (Dex) as a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound.
34297017	4	19	with	hydrogel	478:485	arg1	alginate					499:506	sodium alginate	492:506	sodium alginate (SA)	492:511	In the present study, we successfully synthesized an injectable hydrogel with sodium alginate (SA) and dextran (Dex) as a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound.
34297017	0	20	theme	injectable	15:24	arg1	hydrogels					26:34	PDGF-BB/SA/Dex injectable hydrogels	0:34	PDGF-BB/SA/Dex injectable hydrogels	0:34	PDGF-BB/SA/Dex injectable hydrogels accelerate BMSC-mediated functional full thickness skin wound repair by promoting angiogenesis.
34297017	9	21	theme	PDGF-BB/SA/Dex/BMSCs	1340:1359	arg1	group					1361:1365	the PDGF-BB/SA/Dex/BMSCs group	1336:1365	the PDGF-BB/SA/Dex/BMSCs group	1336:1365	Furthermore, the expressions of angiogenesis-specific markers, PDGFR-β, p-PI3K, p-Akt, and p-eNOS, were obviously increased in the PDGF-BB/SA/Dex/BMSCs group.
34297017	5	22	theme	endothelial	739:749	arg1	cell					751:754	endothelial cell	739:754	endothelial cell (EC) differentiation	739:775	Our work demonstrates that the PDGF-BB protein enhanced the survival, migration and endothelial cell (EC) differentiation of BMSCs in vitro.
34297017	5	22	theme	endothelial	739:749	arg1	EC					757:758	EC	757:758	EC	757:758	Our work demonstrates that the PDGF-BB protein enhanced the survival, migration and endothelial cell (EC) differentiation of BMSCs in vitro.
34297017	0	23	theme	PDGF-BB/SA/Dex	0:13	arg1	hydrogels					26:34	PDGF-BB/SA/Dex injectable hydrogels	0:34	PDGF-BB/SA/Dex injectable hydrogels	0:34	PDGF-BB/SA/Dex injectable hydrogels accelerate BMSC-mediated functional full thickness skin wound repair by promoting angiogenesis.
34297017	8	24	theme	PDGF-BB/SA/Dex	1070:1083	arg1	hydrogels					1085:1093	the PDGF-BB/SA/Dex hydrogels	1066:1093	the PDGF-BB/SA/Dex hydrogels	1066:1093	In addition, the PDGF-BB/SA/Dex hydrogels promoted the EC-differentiation of transplanted BMSCs and proliferation of hair follicle stem cells in the wound.
34297017	9	25	theme	angiogenesis-specific	1241:1261	arg1	p-Akt					1289:1293	p-Akt	1289:1293	p-Akt	1289:1293	Furthermore, the expressions of angiogenesis-specific markers, PDGFR-β, p-PI3K, p-Akt, and p-eNOS, were obviously increased in the PDGF-BB/SA/Dex/BMSCs group.
34297017	9	25	theme	angiogenesis-specific	1241:1261	arg1	p-eNOS					1300:1305	p-eNOS	1300:1305	p-eNOS	1300:1305	Furthermore, the expressions of angiogenesis-specific markers, PDGFR-β, p-PI3K, p-Akt, and p-eNOS, were obviously increased in the PDGF-BB/SA/Dex/BMSCs group.
34297017	9	25	theme	angiogenesis-specific	1241:1261	arg1	p-PI3K					1281:1286	p-PI3K	1281:1286	p-PI3K	1281:1286	Furthermore, the expressions of angiogenesis-specific markers, PDGFR-β, p-PI3K, p-Akt, and p-eNOS, were obviously increased in the PDGF-BB/SA/Dex/BMSCs group.
34297017	9	25	theme	angiogenesis-specific	1241:1261	arg1	PDGFR-β					1272:1278	PDGFR-β	1272:1278	PDGFR-β	1272:1278	Furthermore, the expressions of angiogenesis-specific markers, PDGFR-β, p-PI3K, p-Akt, and p-eNOS, were obviously increased in the PDGF-BB/SA/Dex/BMSCs group.
34297017	9	25	theme	angiogenesis-specific	1241:1261	arg1	markers					1263:1269	angiogenesis-specific markers	1241:1269	angiogenesis-specific markers	1241:1269	Furthermore, the expressions of angiogenesis-specific markers, PDGFR-β, p-PI3K, p-Akt, and p-eNOS, were obviously increased in the PDGF-BB/SA/Dex/BMSCs group.
34297017	2	26	theme	wound	338:342	arg1	healing					344:350	wound healing	338:350	wound healing	338:350	Platelet derived growth factor-BB (PDGF-BB) plays a crucial role in wound healing.
34297017	4	27	theme	delivery	536:543	arg1	system					545:550	a delivery system	534:550	a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound	534:652	In the present study, we successfully synthesized an injectable hydrogel with sodium alginate (SA) and dextran (Dex) as a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound.
34297017	4	27	theme	delivery	536:543	arg1	hydrogel					478:485	an injectable hydrogel	464:485	an injectable hydrogel with sodium alginate (SA) and dextran (Dex)	464:529	In the present study, we successfully synthesized an injectable hydrogel with sodium alginate (SA) and dextran (Dex) as a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound.
34297017	5	28	theme	PDGF-BB	686:692	arg1	protein					694:700	the PDGF-BB protein	682:700	the PDGF-BB protein	682:700	Our work demonstrates that the PDGF-BB protein enhanced the survival, migration and endothelial cell (EC) differentiation of BMSCs in vitro.
34297017	9	29	theme	markers	1263:1269	arg1	expressions					1226:1236	the expressions	1222:1236	the expressions of angiogenesis-specific markers, PDGFR-β, p-PI3K, p-Akt, and p-eNOS,	1222:1306	Furthermore, the expressions of angiogenesis-specific markers, PDGFR-β, p-PI3K, p-Akt, and p-eNOS, were obviously increased in the PDGF-BB/SA/Dex/BMSCs group.
34297017	0	30	dep	thickness	77:85	arg1	repair					98:103	skin wound repair	87:103	BMSC-mediated functional full thickness skin wound repair	47:103	PDGF-BB/SA/Dex injectable hydrogels accelerate BMSC-mediated functional full thickness skin wound repair by promoting angiogenesis.
34297017	1	31	theme	favorable	216:224	arg1	microenvironment					226:241	a favorable microenvironment	214:241	a favorable microenvironment	214:241	Wound healing is a well-orchestrated dynamic and interactive process, which needs a favorable microenvironment and suitable angiogenesis.
34297017	4	32	theme	injectable	467:476	arg1	system					545:550	a delivery system	534:550	a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound	534:652	In the present study, we successfully synthesized an injectable hydrogel with sodium alginate (SA) and dextran (Dex) as a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound.
34297017	4	32	theme	injectable	467:476	arg1	hydrogel					478:485	an injectable hydrogel	464:485	an injectable hydrogel with sodium alginate (SA) and dextran (Dex)	464:529	In the present study, we successfully synthesized an injectable hydrogel with sodium alginate (SA) and dextran (Dex) as a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound.
34297017	1	33	theme	Wound	132:136	arg1	healing					138:144	Wound healing	132:144	Wound healing	132:144	Wound healing is a well-orchestrated dynamic and interactive process, which needs a favorable microenvironment and suitable angiogenesis.
34297017	1	33	theme	Wound	132:136	arg1	process					193:199	a well-orchestrated dynamic and interactive process	149:199	a well-orchestrated dynamic and interactive process	149:199	Wound healing is a well-orchestrated dynamic and interactive process, which needs a favorable microenvironment and suitable angiogenesis.
34297017	0	34	theme	functional	61:70	arg1	thickness					77:85	BMSC-mediated functional full thickness skin wound repair	47:103	BMSC-mediated functional full thickness skin wound repair	47:103	PDGF-BB/SA/Dex injectable hydrogels accelerate BMSC-mediated functional full thickness skin wound repair by promoting angiogenesis.
34297017	10	35	theme	pathway	1564:1570	arg1	activation					1507:1516	the activation	1503:1516	the activation of the PDGF-BB/PDGFR-β-mediated PI3K/Akt/eNOS pathway, which may provide a new therapeutic strategy for stem cell therapy in wound healing	1503:1655	In conclusion, the PDGF-BB/SA/Dex injectable hydrogels could accelerate BMSC-mediated skin wound healing by promoting angiogenesis via the activation of the PDGF-BB/PDGFR-β-mediated PI3K/Akt/eNOS pathway, which may provide a new therapeutic strategy for stem cell therapy in wound healing.
34297017	2	36	theme	derived	279:285	arg1	factor-BB					294:302	Platelet derived growth factor-BB	270:302	Platelet derived growth factor-BB (PDGF-BB)	270:312	Platelet derived growth factor-BB (PDGF-BB) plays a crucial role in wound healing.
34297017	2	36	theme	derived	279:285	arg1	PDGF-BB					305:311	PDGF-BB	305:311	PDGF-BB	305:311	Platelet derived growth factor-BB (PDGF-BB) plays a crucial role in wound healing.
34297017	2	37	dep	derived	279:285	arg1	Platelet					270:277	Platelet	270:277	Platelet	270:277	Platelet derived growth factor-BB (PDGF-BB) plays a crucial role in wound healing.
34297017	0	38	theme	BMSC-mediated	47:59	arg1	thickness					77:85	BMSC-mediated functional full thickness skin wound repair	47:103	BMSC-mediated functional full thickness skin wound repair	47:103	PDGF-BB/SA/Dex injectable hydrogels accelerate BMSC-mediated functional full thickness skin wound repair by promoting angiogenesis.
34297017	10	39	theme	PI3K/Akt/eNOS	1550:1562	arg1	pathway					1564:1570	the PDGF-BB/PDGFR-β-mediated PI3K/Akt/eNOS pathway	1521:1570	the PDGF-BB/PDGFR-β-mediated PI3K/Akt/eNOS pathway	1521:1570	In conclusion, the PDGF-BB/SA/Dex injectable hydrogels could accelerate BMSC-mediated skin wound healing by promoting angiogenesis via the activation of the PDGF-BB/PDGFR-β-mediated PI3K/Akt/eNOS pathway, which may provide a new therapeutic strategy for stem cell therapy in wound healing.
34297017	10	40	theme	therapeutic	1597:1607	arg1	strategy					1609:1616	a new therapeutic strategy	1591:1616	a new therapeutic strategy for stem cell therapy in wound healing	1591:1655	In conclusion, the PDGF-BB/SA/Dex injectable hydrogels could accelerate BMSC-mediated skin wound healing by promoting angiogenesis via the activation of the PDGF-BB/PDGFR-β-mediated PI3K/Akt/eNOS pathway, which may provide a new therapeutic strategy for stem cell therapy in wound healing.
34297017	1	41	theme	suitable	247:254	arg1	angiogenesis					256:267	suitable angiogenesis	247:267	suitable angiogenesis	247:267	Wound healing is a well-orchestrated dynamic and interactive process, which needs a favorable microenvironment and suitable angiogenesis.
34297017	8	42	theme	BMSCs	1143:1147	arg1	EC-differentiation					1108:1125	the EC-differentiation	1104:1125	the EC-differentiation of transplanted BMSCs	1104:1147	In addition, the PDGF-BB/SA/Dex hydrogels promoted the EC-differentiation of transplanted BMSCs and proliferation of hair follicle stem cells in the wound.
34297017	8	42	theme	BMSCs	1143:1147	arg1	proliferation					1153:1165	proliferation	1153:1165	proliferation of hair follicle stem cells	1153:1193	In addition, the PDGF-BB/SA/Dex hydrogels promoted the EC-differentiation of transplanted BMSCs and proliferation of hair follicle stem cells in the wound.
34297017	4	43	link	marrow-derived	595:608	arg1	BMSCs					634:638	BMSCs	634:638	BMSCs	634:638	In the present study, we successfully synthesized an injectable hydrogel with sodium alginate (SA) and dextran (Dex) as a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound.
34297017	4	43	link	marrow-derived	595:608	arg1	cells					627:631	mesenchymal stem cells	610:631	bone marrow-derived mesenchymal stem cells (BMSCs)	590:639	In the present study, we successfully synthesized an injectable hydrogel with sodium alginate (SA) and dextran (Dex) as a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound.
34297017	7	44	theme	collagen	1032:1039	arg1	deposition					1041:1050	collagen deposition	1032:1050	collagen deposition	1032:1050	Besides, these composite hydrogels loaded with BMSCs could accelerate wound healing by improving epithelialization and collagen deposition.
34297017	0	45	theme	full	72:75	arg1	thickness					77:85	BMSC-mediated functional full thickness skin wound repair	47:103	BMSC-mediated functional full thickness skin wound repair	47:103	PDGF-BB/SA/Dex injectable hydrogels accelerate BMSC-mediated functional full thickness skin wound repair by promoting angiogenesis.
34297017	8	46	theme	transplanted	1130:1141	arg1	BMSCs					1143:1147	transplanted BMSCs	1130:1147	transplanted BMSCs	1130:1147	In addition, the PDGF-BB/SA/Dex hydrogels promoted the EC-differentiation of transplanted BMSCs and proliferation of hair follicle stem cells in the wound.
34297017	10	47	from	therapy	1632:1638	arg1	healing					1649:1655	wound healing	1643:1655	wound healing	1643:1655	In conclusion, the PDGF-BB/SA/Dex injectable hydrogels could accelerate BMSC-mediated skin wound healing by promoting angiogenesis via the activation of the PDGF-BB/PDGFR-β-mediated PI3K/Akt/eNOS pathway, which may provide a new therapeutic strategy for stem cell therapy in wound healing.
34297017	10	48	theme	wound	1459:1463	arg1	healing					1465:1471	BMSC-mediated skin wound healing	1440:1471	BMSC-mediated skin wound healing	1440:1471	In conclusion, the PDGF-BB/SA/Dex injectable hydrogels could accelerate BMSC-mediated skin wound healing by promoting angiogenesis via the activation of the PDGF-BB/PDGFR-β-mediated PI3K/Akt/eNOS pathway, which may provide a new therapeutic strategy for stem cell therapy in wound healing.
34297017	10	49	theme	cell	1627:1630	arg1	therapy					1632:1638	stem cell therapy	1622:1638	stem cell therapy in wound healing	1622:1655	In conclusion, the PDGF-BB/SA/Dex injectable hydrogels could accelerate BMSC-mediated skin wound healing by promoting angiogenesis via the activation of the PDGF-BB/PDGFR-β-mediated PI3K/Akt/eNOS pathway, which may provide a new therapeutic strategy for stem cell therapy in wound healing.
34297017	10	50	theme	skin	1454:1457	arg1	healing					1465:1471	BMSC-mediated skin wound healing	1440:1471	BMSC-mediated skin wound healing	1440:1471	In conclusion, the PDGF-BB/SA/Dex injectable hydrogels could accelerate BMSC-mediated skin wound healing by promoting angiogenesis via the activation of the PDGF-BB/PDGFR-β-mediated PI3K/Akt/eNOS pathway, which may provide a new therapeutic strategy for stem cell therapy in wound healing.
34297017	5	51	dep	survival	715:722	arg1	the					711:713	the	711:713	the	711:713	Our work demonstrates that the PDGF-BB protein enhanced the survival, migration and endothelial cell (EC) differentiation of BMSCs in vitro.
34297017	8	52	theme	cells	1189:1193	arg1	EC-differentiation					1108:1125	the EC-differentiation	1104:1125	the EC-differentiation of transplanted BMSCs	1104:1147	In addition, the PDGF-BB/SA/Dex hydrogels promoted the EC-differentiation of transplanted BMSCs and proliferation of hair follicle stem cells in the wound.
34297017	8	52	theme	cells	1189:1193	arg1	proliferation					1153:1165	proliferation	1153:1165	proliferation of hair follicle stem cells	1153:1193	In addition, the PDGF-BB/SA/Dex hydrogels promoted the EC-differentiation of transplanted BMSCs and proliferation of hair follicle stem cells in the wound.
34297017	5	53	theme	cell	751:754	arg1	differentiation					761:775	endothelial cell (EC) differentiation	739:775	endothelial cell (EC) differentiation	739:775	Our work demonstrates that the PDGF-BB protein enhanced the survival, migration and endothelial cell (EC) differentiation of BMSCs in vitro.
34297017	4	54	theme	mesenchymal	610:620	arg1	BMSCs					634:638	BMSCs	634:638	BMSCs	634:638	In the present study, we successfully synthesized an injectable hydrogel with sodium alginate (SA) and dextran (Dex) as a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound.
34297017	4	54	theme	mesenchymal	610:620	arg1	cells					627:631	mesenchymal stem cells	610:631	bone marrow-derived mesenchymal stem cells (BMSCs)	590:639	In the present study, we successfully synthesized an injectable hydrogel with sodium alginate (SA) and dextran (Dex) as a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound.
34297017	10	55	theme	injectable	1402:1411	arg1	hydrogels					1413:1421	the PDGF-BB/SA/Dex injectable hydrogels	1383:1421	the PDGF-BB/SA/Dex injectable hydrogels	1383:1421	In conclusion, the PDGF-BB/SA/Dex injectable hydrogels could accelerate BMSC-mediated skin wound healing by promoting angiogenesis via the activation of the PDGF-BB/PDGFR-β-mediated PI3K/Akt/eNOS pathway, which may provide a new therapeutic strategy for stem cell therapy in wound healing.
34297017	1	56	theme	well-orchestrated	151:167	arg1	healing					138:144	Wound healing	132:144	Wound healing	132:144	Wound healing is a well-orchestrated dynamic and interactive process, which needs a favorable microenvironment and suitable angiogenesis.
34297017	1	56	theme	well-orchestrated	151:167	arg1	process					193:199	a well-orchestrated dynamic and interactive process	149:199	a well-orchestrated dynamic and interactive process	149:199	Wound healing is a well-orchestrated dynamic and interactive process, which needs a favorable microenvironment and suitable angiogenesis.
34297017	4	57	theme	marrow-derived	595:608	arg1	BMSCs					634:638	BMSCs	634:638	BMSCs	634:638	In the present study, we successfully synthesized an injectable hydrogel with sodium alginate (SA) and dextran (Dex) as a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound.
34297017	4	57	theme	marrow-derived	595:608	arg1	cells					627:631	mesenchymal stem cells	610:631	bone marrow-derived mesenchymal stem cells (BMSCs)	590:639	In the present study, we successfully synthesized an injectable hydrogel with sodium alginate (SA) and dextran (Dex) as a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound.
34297017	0	58	theme	wound	92:96	arg1	repair					98:103	skin wound repair	87:103	BMSC-mediated functional full thickness skin wound repair	47:103	PDGF-BB/SA/Dex injectable hydrogels accelerate BMSC-mediated functional full thickness skin wound repair by promoting angiogenesis.
34297017	6	59	theme	PDGF-BB/SA/Dex	800:813	arg1	hydrogels					815:823	The PDGF-BB/SA/Dex hydrogels	796:823	The PDGF-BB/SA/Dex hydrogels	796:823	The PDGF-BB/SA/Dex hydrogels could sustainably release PDGF-BB with excellent biocompatibility in vitro and in vivo.
34297017	10	60	theme	PDGF-BB/SA/Dex	1387:1400	arg1	hydrogels					1413:1421	the PDGF-BB/SA/Dex injectable hydrogels	1383:1421	the PDGF-BB/SA/Dex injectable hydrogels	1383:1421	In conclusion, the PDGF-BB/SA/Dex injectable hydrogels could accelerate BMSC-mediated skin wound healing by promoting angiogenesis via the activation of the PDGF-BB/PDGFR-β-mediated PI3K/Akt/eNOS pathway, which may provide a new therapeutic strategy for stem cell therapy in wound healing.
34297017	1	61	theme	dynamic	169:175	arg1	healing					138:144	Wound healing	132:144	Wound healing	132:144	Wound healing is a well-orchestrated dynamic and interactive process, which needs a favorable microenvironment and suitable angiogenesis.
34297017	1	61	theme	dynamic	169:175	arg1	process					193:199	a well-orchestrated dynamic and interactive process	149:199	a well-orchestrated dynamic and interactive process	149:199	Wound healing is a well-orchestrated dynamic and interactive process, which needs a favorable microenvironment and suitable angiogenesis.
34297017	2	62	link	derived	279:285	arg1	factor-BB					294:302	Platelet derived growth factor-BB	270:302	Platelet derived growth factor-BB (PDGF-BB)	270:312	Platelet derived growth factor-BB (PDGF-BB) plays a crucial role in wound healing.
34297017	2	62	link	derived	279:285	arg1	PDGF-BB					305:311	PDGF-BB	305:311	PDGF-BB	305:311	Platelet derived growth factor-BB (PDGF-BB) plays a crucial role in wound healing.
34297017	0	63	theme	skin	87:90	arg1	repair					98:103	skin wound repair	87:103	BMSC-mediated functional full thickness skin wound repair	47:103	PDGF-BB/SA/Dex injectable hydrogels accelerate BMSC-mediated functional full thickness skin wound repair by promoting angiogenesis.
34297017	2	64	theme	growth	287:292	arg1	factor-BB					294:302	Platelet derived growth factor-BB	270:302	Platelet derived growth factor-BB (PDGF-BB)	270:312	Platelet derived growth factor-BB (PDGF-BB) plays a crucial role in wound healing.
34297017	2	64	theme	growth	287:292	arg1	PDGF-BB					305:311	PDGF-BB	305:311	PDGF-BB	305:311	Platelet derived growth factor-BB (PDGF-BB) plays a crucial role in wound healing.
34297017	4	65	theme	stem	622:625	arg1	BMSCs					634:638	BMSCs	634:638	BMSCs	634:638	In the present study, we successfully synthesized an injectable hydrogel with sodium alginate (SA) and dextran (Dex) as a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound.
34297017	4	65	theme	stem	622:625	arg1	cells					627:631	mesenchymal stem cells	610:631	bone marrow-derived mesenchymal stem cells (BMSCs)	590:639	In the present study, we successfully synthesized an injectable hydrogel with sodium alginate (SA) and dextran (Dex) as a delivery system to simultaneously deliver PDGF-BB and bone marrow-derived mesenchymal stem cells (BMSCs) in the wound.
34648780	0	0	theme	study	104:108	arg1	Development					76:86	Development	76:86	Boron removal from aqueous solutions by chitosan/functionalized-SWCNT-COOH: Development of optimization study using response surface methodology and simulated annealing.	0:168	Boron removal from aqueous solutions by chitosan/functionalized-SWCNT-COOH: Development of optimization study using response surface methodology and simulated annealing.
34648780	11	1	theme	adsorption	1644:1653	arg1	62.06 mg g-1					1686:1697	62.06 mg g-1	1686:1697	62.06 mg g-1	1686:1697	The optimal adsorption capacity of SA was found to be 62.06 mg g-1.
34648780	11	1	theme	adsorption	1644:1653	arg1	capacity					1655:1662	The optimal adsorption capacity	1632:1662	The optimal adsorption capacity of SA	1632:1668	The optimal adsorption capacity of SA was found to be 62.06 mg g-1.
34648780	0	2	theme	optimization	91:102	arg1	study					104:108	optimization study	91:108	optimization study using response surface methodology and simulated annealing	91:167	Boron removal from aqueous solutions by chitosan/functionalized-SWCNT-COOH: Development of optimization study using response surface methodology and simulated annealing.
34648780	6	3	dep	conditions	1006:1015	arg1	98.77 mg L-1					1018:1029	98.77 mg L-1	1018:1029	98.77 mg L-1 of boric acid concentration, pH of 5.46 and 76 min	1018:1080	The optimized response (adsorption capacity) was reached 62.16 mg g-1 under the optimal conditions (98.77 mg L-1 of boric acid concentration, pH of 5.46 and 76 min).
34648780	5	4	theme	Response	674:681	arg1	RSM					699:701	RSM	699:701	RSM	699:701	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	5	4	theme	Response	674:681	arg1	method					691:696	Response surface method	674:696	Response surface method (RSM) with face-centered central composite design (FCCCD)	674:754	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	1	5	theme	water	193:197	arg1	resources					199:207	water resources	193:207	water resources (especially drinking waters and agricultural land)	193:258	Boron contamination in water resources (especially drinking waters and agricultural land) is a major problem for the ecosystem.
34648780	6	6	theme	optimal	998:1004	arg1	conditions					1006:1015	the optimal conditions	994:1015	the optimal conditions (98.77 mg L-1 of boric acid concentration, pH of 5.46 and 76 min)	994:1081	The optimized response (adsorption capacity) was reached 62.16 mg g-1 under the optimal conditions (98.77 mg L-1 of boric acid concentration, pH of 5.46 and 76 min).
34648780	0	7	theme	response	116:123	arg1	methodology					133:143	response surface methodology	116:143	response surface methodology	116:143	Boron removal from aqueous solutions by chitosan/functionalized-SWCNT-COOH: Development of optimization study using response surface methodology and simulated annealing.
34648780	7	8	theme	existing	1313:1320	arg1	adsorbents					1322:1331	existing adsorbents	1313:1331	existing adsorbents	1313:1331	The present study has indicated that the synthesized material can be used as an adsorbent for eliminating boric acid from aqueous solutions depending on its high adsorbent capacity to remove boron and has better performance than existing adsorbents.
34648780	5	9	theme	surface	683:689	arg1	RSM					699:701	RSM	699:701	RSM	699:701	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	5	9	theme	surface	683:689	arg1	method					691:696	Response surface method	674:696	Response surface method (RSM) with face-centered central composite design (FCCCD)	674:754	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	7	10	theme	better	1289:1294	arg1	performance					1296:1306	better performance	1289:1306	better performance than existing adsorbents	1289:1331	The present study has indicated that the synthesized material can be used as an adsorbent for eliminating boric acid from aqueous solutions depending on its high adsorbent capacity to remove boron and has better performance than existing adsorbents.
34648780	7	11	theme	boric	1190:1194	arg1	acid					1196:1199	boric acid	1190:1199	boric acid from aqueous solutions	1190:1222	The present study has indicated that the synthesized material can be used as an adsorbent for eliminating boric acid from aqueous solutions depending on its high adsorbent capacity to remove boron and has better performance than existing adsorbents.
34648780	7	12	dep	capacity	1256:1263	arg1	has					1285:1287	has	1285:1287	has better performance than existing adsorbents	1285:1331	The present study has indicated that the synthesized material can be used as an adsorbent for eliminating boric acid from aqueous solutions depending on its high adsorbent capacity to remove boron and has better performance than existing adsorbents.
34648780	7	12	dep	capacity	1256:1263	arg1	remove					1268:1273	remove	1268:1273	to remove boron	1265:1279	The present study has indicated that the synthesized material can be used as an adsorbent for eliminating boric acid from aqueous solutions depending on its high adsorbent capacity to remove boron and has better performance than existing adsorbents.
34648780	1	13	from	contamination	176:188	arg1	resources					199:207	water resources	193:207	water resources (especially drinking waters and agricultural land)	193:258	Boron contamination in water resources (especially drinking waters and agricultural land) is a major problem for the ecosystem.
34648780	6	14	theme	boric	1034:1038	arg1	acid					1040:1043	boric acid	1034:1043	boric acid concentration	1034:1057	The optimized response (adsorption capacity) was reached 62.16 mg g-1 under the optimal conditions (98.77 mg L-1 of boric acid concentration, pH of 5.46 and 76 min).
34648780	6	15	theme	5.46	1066:1069	arg1	pH					1060:1061	pH	1060:1061	pH of 5.46	1060:1069	The optimized response (adsorption capacity) was reached 62.16 mg g-1 under the optimal conditions (98.77 mg L-1 of boric acid concentration, pH of 5.46 and 76 min).
34648780	6	15	theme	5.46	1066:1069	arg1	concentration					1045:1057	boric acid concentration	1034:1057	boric acid concentration	1034:1057	The optimized response (adsorption capacity) was reached 62.16 mg g-1 under the optimal conditions (98.77 mg L-1 of boric acid concentration, pH of 5.46 and 76 min).
34648780	6	15	theme	5.46	1066:1069	arg1	76 min					1075:1080	76 min	1075:1080	76 min	1075:1080	The optimized response (adsorption capacity) was reached 62.16 mg g-1 under the optimal conditions (98.77 mg L-1 of boric acid concentration, pH of 5.46 and 76 min).
34648780	6	16	theme	76 min	1075:1080	arg1	98.77 mg L-1					1018:1029	98.77 mg L-1	1018:1029	98.77 mg L-1 of boric acid concentration, pH of 5.46 and 76 min	1018:1080	The optimized response (adsorption capacity) was reached 62.16 mg g-1 under the optimal conditions (98.77 mg L-1 of boric acid concentration, pH of 5.46 and 76 min).
34648780	0	17	theme	surface	125:131	arg1	methodology					133:143	response surface methodology	116:143	response surface methodology	116:143	Boron removal from aqueous solutions by chitosan/functionalized-SWCNT-COOH: Development of optimization study using response surface methodology and simulated annealing.
34648780	5	18	theme	boric	906:910	arg1	acid					912:915	boric acid	906:915	boric acid	906:915	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	3	19	theme	microscopy	457:466	arg1	analysis					502:509	Scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis	439:509	Scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis	439:509	Scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis revealed that SWCNT was dispersed in chitosan homogenously.
34648780	9	20	theme	error	1502:1506	arg1	functions					1508:1516	error functions	1502:1516	error functions	1502:1516	Moreover, the selected optimization techniques were compared with error functions.
34648780	10	21	theme	concentration	1590:1602	arg1	91.17 mg L-1					1563:1574	91.17 mg L-1	1563:1574	91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min	1563:1629	The optimal conditions derived from SA were 91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min.
34648780	10	21	theme	concentration	1590:1602	arg1	conditions					1531:1540	The optimal conditions	1519:1540	The optimal conditions derived from SA	1519:1556	The optimal conditions derived from SA were 91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min.
34648780	5	22	theme	adsorbent	869:877	arg1	dose					879:882	adsorbent dose	869:882	adsorbent dose	869:882	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	0	23	from	solutions	27:35	arg1	removal					6:12	Boron removal	0:12	Boron removal from aqueous solutions by chitosan/functionalized-SWCNT-COOH: Development of optimization study using response surface methodology and simulated annealing.	0:168	Boron removal from aqueous solutions by chitosan/functionalized-SWCNT-COOH: Development of optimization study using response surface methodology and simulated annealing.
34648780	2	24	theme	synthesized	321:331	arg1	chitosan/functionalized-SWCNT-COOH					333:366	a novel synthesized chitosan/functionalized-SWCNT-COOH	313:366	a novel synthesized chitosan/functionalized-SWCNT-COOH	313:366	In this study, a novel synthesized chitosan/functionalized-SWCNT-COOH was prepared to separate boron (as boric acid) from aqueous solutions.
34648780	10	25	theme	acid	1585:1588	arg1	concentration					1590:1602	boric acid concentration	1579:1602	boric acid concentration	1579:1602	The optimal conditions derived from SA were 91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min.
34648780	3	26	theme	electron	448:455	arg1	SEM					469:471	SEM	469:471	SEM	469:471	Scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis revealed that SWCNT was dispersed in chitosan homogenously.
34648780	3	26	theme	electron	448:455	arg1	microscopy					457:466	Scanning electron microscopy	439:466	Scanning electron microscopy (SEM)	439:472	Scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis revealed that SWCNT was dispersed in chitosan homogenously.
34648780	0	27	theme	Boron	0:4	arg1	removal					6:12	Boron removal	0:12	Boron removal from aqueous solutions by chitosan/functionalized-SWCNT-COOH: Development of optimization study using response surface methodology and simulated annealing.	0:168	Boron removal from aqueous solutions by chitosan/functionalized-SWCNT-COOH: Development of optimization study using response surface methodology and simulated annealing.
34648780	10	28	theme	pH	1605:1606	arg1	91.17 mg L-1					1563:1574	91.17 mg L-1	1563:1574	91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min	1563:1629	The optimal conditions derived from SA were 91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min.
34648780	10	28	theme	pH	1605:1606	arg1	conditions					1531:1540	The optimal conditions	1519:1540	The optimal conditions derived from SA	1519:1556	The optimal conditions derived from SA were 91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min.
34648780	7	29	from	solutions	1214:1222	arg1	acid					1196:1199	boric acid	1190:1199	boric acid from aqueous solutions	1190:1222	The present study has indicated that the synthesized material can be used as an adsorbent for eliminating boric acid from aqueous solutions depending on its high adsorbent capacity to remove boron and has better performance than existing adsorbents.
34648780	2	30	theme	novel	315:319	arg1	chitosan/functionalized-SWCNT-COOH					333:366	a novel synthesized chitosan/functionalized-SWCNT-COOH	313:366	a novel synthesized chitosan/functionalized-SWCNT-COOH	313:366	In this study, a novel synthesized chitosan/functionalized-SWCNT-COOH was prepared to separate boron (as boric acid) from aqueous solutions.
34648780	8	31	theme	simulated	1347:1355	arg1	SA					1368:1369	SA	1368:1369	SA	1368:1369	Furthermore, simulated annealing (SA) optimization technique was used to compare the findings of RSM.
34648780	8	31	theme	simulated	1347:1355	arg1	annealing					1357:1365	simulated annealing	1347:1365	simulated annealing (SA) optimization technique	1347:1393	Furthermore, simulated annealing (SA) optimization technique was used to compare the findings of RSM.
34648780	2	32	theme	boric	403:407	arg1	acid					409:412	boric acid	403:412	boric acid	403:412	In this study, a novel synthesized chitosan/functionalized-SWCNT-COOH was prepared to separate boron (as boric acid) from aqueous solutions.
34648780	0	33	theme	aqueous	19:25	arg1	solutions					27:35	aqueous solutions	19:35	aqueous solutions	19:35	Boron removal from aqueous solutions by chitosan/functionalized-SWCNT-COOH: Development of optimization study using response surface methodology and simulated annealing.
34648780	0	34	theme	simulated	149:157	arg1	annealing					159:167	simulated annealing	149:167	simulated annealing	149:167	Boron removal from aqueous solutions by chitosan/functionalized-SWCNT-COOH: Development of optimization study using response surface methodology and simulated annealing.
34648780	10	35	theme	76.17 min	1621:1629	arg1	91.17 mg L-1					1563:1574	91.17 mg L-1	1563:1574	91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min	1563:1629	The optimal conditions derived from SA were 91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min.
34648780	10	35	theme	76.17 min	1621:1629	arg1	conditions					1531:1540	The optimal conditions	1519:1540	The optimal conditions derived from SA	1519:1556	The optimal conditions derived from SA were 91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min.
34648780	10	36	attach	derived	1542:1548	arg2	91.17 mg L-1					1563:1574	91.17 mg L-1	1563:1574	91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min	1563:1629	The optimal conditions derived from SA were 91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min.
34648780	10	36	attach	derived	1542:1548	arg1	SA					1555:1556	SA	1555:1556	SA	1555:1556	The optimal conditions derived from SA were 91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min.
34648780	10	36	attach	derived	1542:1548	arg2	conditions					1531:1540	The optimal conditions	1519:1540	The optimal conditions derived from SA	1519:1556	The optimal conditions derived from SA were 91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min.
34648780	3	37	theme	X-ray	478:482	arg1	XRD					497:499	XRD	497:499	XRD	497:499	Scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis revealed that SWCNT was dispersed in chitosan homogenously.
34648780	3	37	theme	X-ray	478:482	arg1	diffraction					484:494	X-ray diffraction	478:494	X-ray diffraction (XRD)	478:500	Scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis revealed that SWCNT was dispersed in chitosan homogenously.
34648780	5	38	theme	independent	837:847	arg1	factors					849:855	optimal independent factors	829:855	optimal independent factors such as pH, adsorbent dose, and concentration of boric acid	829:915	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	5	38	theme	independent	837:847	arg1	pH					865:866	pH	865:866	pH	865:866	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	5	38	theme	independent	837:847	arg1	dose					879:882	adsorbent dose	869:882	adsorbent dose	869:882	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	5	38	theme	independent	837:847	arg1	concentration					889:901	concentration	889:901	concentration of boric acid	889:915	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	9	39	theme	selected	1450:1457	arg1	techniques					1472:1481	the selected optimization techniques	1446:1481	the selected optimization techniques	1446:1481	Moreover, the selected optimization techniques were compared with error functions.
34648780	5	40	theme	central	723:729	arg1	FCCCD					749:753	FCCCD	749:753	FCCCD	749:753	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	5	40	theme	central	723:729	arg1	design					741:746	face-centered central composite design	709:746	face-centered central composite design (FCCCD)	709:754	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	8	41	theme	RSM	1431:1433	arg1	findings					1419:1426	the findings	1415:1426	the findings of RSM	1415:1433	Furthermore, simulated annealing (SA) optimization technique was used to compare the findings of RSM.
34648780	2	42	theme	separate	384:391	arg1	boron					393:397	separate boron	384:397	separate boron (as boric acid) from aqueous solutions	384:436	In this study, a novel synthesized chitosan/functionalized-SWCNT-COOH was prepared to separate boron (as boric acid) from aqueous solutions.
34648780	3	43	theme	diffraction	484:494	arg1	analysis					502:509	Scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis	439:509	Scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis	439:509	Scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis revealed that SWCNT was dispersed in chitosan homogenously.
34648780	5	44	theme	adsorption	786:795	arg1	capacity					797:804	the adsorption capacity	782:804	the adsorption capacity	782:804	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	11	45	theme	optimal	1636:1642	arg1	62.06 mg g-1					1686:1697	62.06 mg g-1	1686:1697	62.06 mg g-1	1686:1697	The optimal adsorption capacity of SA was found to be 62.06 mg g-1.
34648780	11	45	theme	optimal	1636:1642	arg1	capacity					1655:1662	The optimal adsorption capacity	1632:1662	The optimal adsorption capacity of SA	1632:1668	The optimal adsorption capacity of SA was found to be 62.06 mg g-1.
34648780	5	46	theme	composite	731:739	arg1	FCCCD					749:753	FCCCD	749:753	FCCCD	749:753	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	5	46	theme	composite	731:739	arg1	design					741:746	face-centered central composite design	709:746	face-centered central composite design (FCCCD)	709:754	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	6	47	theme	concentration	1045:1057	arg1	98.77 mg L-1					1018:1029	98.77 mg L-1	1018:1029	98.77 mg L-1 of boric acid concentration, pH of 5.46 and 76 min	1018:1080	The optimized response (adsorption capacity) was reached 62.16 mg g-1 under the optimal conditions (98.77 mg L-1 of boric acid concentration, pH of 5.46 and 76 min).
34648780	4	48	with	problem	636:642	arg1	approach					664:671	an engineering approach	649:671	an engineering approach	649:671	Moreover, this study has related to the constrained optimization problem with an engineering approach.
34648780	1	49	theme	drinking	221:228	arg1	waters					230:235	especially drinking waters	210:235	especially drinking waters	210:235	Boron contamination in water resources (especially drinking waters and agricultural land) is a major problem for the ecosystem.
34648780	1	50	theme	agricultural	241:252	arg1	land					254:257	agricultural land	241:257	agricultural land	241:257	Boron contamination in water resources (especially drinking waters and agricultural land) is a major problem for the ecosystem.
34648780	3	51	theme	Scanning	439:446	arg1	SEM					469:471	SEM	469:471	SEM	469:471	Scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis revealed that SWCNT was dispersed in chitosan homogenously.
34648780	3	51	theme	Scanning	439:446	arg1	microscopy					457:466	Scanning electron microscopy	439:466	Scanning electron microscopy (SEM)	439:472	Scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis revealed that SWCNT was dispersed in chitosan homogenously.
34648780	10	52	theme	5.86	1611:1614	arg1	concentration					1590:1602	boric acid concentration	1579:1602	boric acid concentration	1579:1602	The optimal conditions derived from SA were 91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min.
34648780	10	52	theme	5.86	1611:1614	arg1	76.17 min					1621:1629	76.17 min	1621:1629	76.17 min	1621:1629	The optimal conditions derived from SA were 91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min.
34648780	10	52	theme	5.86	1611:1614	arg1	pH					1605:1606	pH	1605:1606	pH of 5.86	1605:1614	The optimal conditions derived from SA were 91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min.
34648780	9	53	theme	optimization	1459:1470	arg1	techniques					1472:1481	the selected optimization techniques	1446:1481	the selected optimization techniques	1446:1481	Moreover, the selected optimization techniques were compared with error functions.
34648780	5	54	theme	optimal	829:835	arg1	factors					849:855	optimal independent factors	829:855	optimal independent factors such as pH, adsorbent dose, and concentration of boric acid	829:915	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	5	54	theme	optimal	829:835	arg1	pH					865:866	pH	865:866	pH	865:866	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	5	54	theme	optimal	829:835	arg1	dose					879:882	adsorbent dose	869:882	adsorbent dose	869:882	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	5	54	theme	optimal	829:835	arg1	concentration					889:901	concentration	889:901	concentration of boric acid	889:915	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	2	55	theme	aqueous	420:426	arg1	solutions					428:436	aqueous solutions	420:436	aqueous solutions	420:436	In this study, a novel synthesized chitosan/functionalized-SWCNT-COOH was prepared to separate boron (as boric acid) from aqueous solutions.
34648780	6	56	theme	adsorption	942:951	arg1	response					932:939	The optimized response	918:939	The optimized response (adsorption capacity)	918:961	The optimized response (adsorption capacity) was reached 62.16 mg g-1 under the optimal conditions (98.77 mg L-1 of boric acid concentration, pH of 5.46 and 76 min).
34648780	6	56	theme	adsorption	942:951	arg1	capacity					953:960	adsorption capacity	942:960	adsorption capacity	942:960	The optimized response (adsorption capacity) was reached 62.16 mg g-1 under the optimal conditions (98.77 mg L-1 of boric acid concentration, pH of 5.46 and 76 min).
34648780	6	57	theme	acid	1040:1043	arg1	concentration					1045:1057	boric acid concentration	1034:1057	boric acid concentration	1034:1057	The optimized response (adsorption capacity) was reached 62.16 mg g-1 under the optimal conditions (98.77 mg L-1 of boric acid concentration, pH of 5.46 and 76 min).
34648780	1	58	dep	resources	199:207	arg1	waters					230:235	especially drinking waters	210:235	especially drinking waters	210:235	Boron contamination in water resources (especially drinking waters and agricultural land) is a major problem for the ecosystem.
34648780	1	58	dep	resources	199:207	arg1	land					254:257	agricultural land	241:257	agricultural land	241:257	Boron contamination in water resources (especially drinking waters and agricultural land) is a major problem for the ecosystem.
34648780	8	59	theme	annealing	1357:1365	arg1	technique					1385:1393	simulated annealing (SA) optimization technique	1347:1393	simulated annealing (SA) optimization technique	1347:1393	Furthermore, simulated annealing (SA) optimization technique was used to compare the findings of RSM.
34648780	5	60	theme	acid	912:915	arg1	pH					865:866	pH	865:866	pH	865:866	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	5	60	theme	acid	912:915	arg1	dose					879:882	adsorbent dose	869:882	adsorbent dose	869:882	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	5	60	theme	acid	912:915	arg1	concentration					889:901	concentration	889:901	concentration of boric acid	889:915	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	7	61	theme	synthesized	1125:1135	arg1	material					1137:1144	the synthesized material	1121:1144	the synthesized material	1121:1144	The present study has indicated that the synthesized material can be used as an adsorbent for eliminating boric acid from aqueous solutions depending on its high adsorbent capacity to remove boron and has better performance than existing adsorbents.
34648780	7	61	theme	synthesized	1125:1135	arg1	adsorbent					1164:1172	adsorbent	1164:1172	adsorbent	1164:1172	The present study has indicated that the synthesized material can be used as an adsorbent for eliminating boric acid from aqueous solutions depending on its high adsorbent capacity to remove boron and has better performance than existing adsorbents.
34648780	5	62	theme	face-centered	709:721	arg1	FCCCD					749:753	FCCCD	749:753	FCCCD	749:753	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	5	62	theme	face-centered	709:721	arg1	design					741:746	face-centered central composite design	709:746	face-centered central composite design (FCCCD)	709:754	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	7	63	theme	present	1088:1094	arg1	study					1096:1100	The present study	1084:1100	The present study	1084:1100	The present study has indicated that the synthesized material can be used as an adsorbent for eliminating boric acid from aqueous solutions depending on its high adsorbent capacity to remove boron and has better performance than existing adsorbents.
34648780	8	64	used	used	1399:1402	arg2	technique					1385:1393	simulated annealing (SA) optimization technique	1347:1393	simulated annealing (SA) optimization technique	1347:1393	Furthermore, simulated annealing (SA) optimization technique was used to compare the findings of RSM.
34648780	7	65	theme	high	1241:1244	arg1	capacity					1256:1263	its high adsorbent capacity	1237:1263	its high adsorbent capacity to remove boron and has better performance than existing adsorbents	1237:1331	The present study has indicated that the synthesized material can be used as an adsorbent for eliminating boric acid from aqueous solutions depending on its high adsorbent capacity to remove boron and has better performance than existing adsorbents.
34648780	11	66	theme	SA	1667:1668	arg1	62.06 mg g-1					1686:1697	62.06 mg g-1	1686:1697	62.06 mg g-1	1686:1697	The optimal adsorption capacity of SA was found to be 62.06 mg g-1.
34648780	11	66	theme	SA	1667:1668	arg1	capacity					1655:1662	The optimal adsorption capacity	1632:1662	The optimal adsorption capacity of SA	1632:1668	The optimal adsorption capacity of SA was found to be 62.06 mg g-1.
34648780	8	67	theme	optimization	1372:1383	arg1	technique					1385:1393	simulated annealing (SA) optimization technique	1347:1393	simulated annealing (SA) optimization technique	1347:1393	Furthermore, simulated annealing (SA) optimization technique was used to compare the findings of RSM.
34648780	7	68	theme	adsorbent	1246:1254	arg1	capacity					1256:1263	its high adsorbent capacity	1237:1263	its high adsorbent capacity to remove boron and has better performance than existing adsorbents	1237:1331	The present study has indicated that the synthesized material can be used as an adsorbent for eliminating boric acid from aqueous solutions depending on its high adsorbent capacity to remove boron and has better performance than existing adsorbents.
34648780	4	69	theme	constrained	611:621	arg1	problem					636:642	the constrained optimization problem	607:642	the constrained optimization problem with an engineering approach	607:671	Moreover, this study has related to the constrained optimization problem with an engineering approach.
34648780	2	70	from	solutions	428:436	arg1	boron					393:397	separate boron	384:397	separate boron (as boric acid) from aqueous solutions	384:436	In this study, a novel synthesized chitosan/functionalized-SWCNT-COOH was prepared to separate boron (as boric acid) from aqueous solutions.
34648780	7	71	used	used	1153:1156	arg2	adsorbent					1164:1172	adsorbent	1164:1172	adsorbent	1164:1172	The present study has indicated that the synthesized material can be used as an adsorbent for eliminating boric acid from aqueous solutions depending on its high adsorbent capacity to remove boron and has better performance than existing adsorbents.
34648780	7	71	used	used	1153:1156	arg2	material					1137:1144	the synthesized material	1121:1144	the synthesized material	1121:1144	The present study has indicated that the synthesized material can be used as an adsorbent for eliminating boric acid from aqueous solutions depending on its high adsorbent capacity to remove boron and has better performance than existing adsorbents.
34648780	12	72	theme	models	1755:1760	arg1	other					1785:1789	other	1785:1789	other	1785:1789	These results revealed that the predictions of the two models are very close to each other.
34648780	12	72	theme	models	1755:1760	arg1	predictions					1732:1742	the predictions	1728:1742	the predictions of the two models	1728:1760	These results revealed that the predictions of the two models are very close to each other.
34648780	7	73	theme	aqueous	1206:1212	arg1	solutions					1214:1222	aqueous solutions	1206:1222	aqueous solutions	1206:1222	The present study has indicated that the synthesized material can be used as an adsorbent for eliminating boric acid from aqueous solutions depending on its high adsorbent capacity to remove boron and has better performance than existing adsorbents.
34648780	4	74	theme	engineering	652:662	arg1	approach					664:671	an engineering approach	649:671	an engineering approach	649:671	Moreover, this study has related to the constrained optimization problem with an engineering approach.
34648780	0	75	dep	removal	6:12	arg1	Development					76:86	Development	76:86	Boron removal from aqueous solutions by chitosan/functionalized-SWCNT-COOH: Development of optimization study using response surface methodology and simulated annealing.	0:168	Boron removal from aqueous solutions by chitosan/functionalized-SWCNT-COOH: Development of optimization study using response surface methodology and simulated annealing.
34648780	6	76	theme	pH	1060:1061	arg1	98.77 mg L-1					1018:1029	98.77 mg L-1	1018:1029	98.77 mg L-1 of boric acid concentration, pH of 5.46 and 76 min	1018:1080	The optimized response (adsorption capacity) was reached 62.16 mg g-1 under the optimal conditions (98.77 mg L-1 of boric acid concentration, pH of 5.46 and 76 min).
34648780	1	77	theme	Boron	170:174	arg1	contamination					176:188	Boron contamination	170:188	Boron contamination in water resources (especially drinking waters and agricultural land)	170:258	Boron contamination in water resources (especially drinking waters and agricultural land) is a major problem for the ecosystem.
34648780	1	77	theme	Boron	170:174	arg1	problem					271:277	a major problem	263:277	a major problem for the ecosystem	263:295	Boron contamination in water resources (especially drinking waters and agricultural land) is a major problem for the ecosystem.
34648780	10	78	theme	optimal	1523:1529	arg1	91.17 mg L-1					1563:1574	91.17 mg L-1	1563:1574	91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min	1563:1629	The optimal conditions derived from SA were 91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min.
34648780	10	78	theme	optimal	1523:1529	arg1	conditions					1531:1540	The optimal conditions	1519:1540	The optimal conditions derived from SA	1519:1556	The optimal conditions derived from SA were 91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min.
34648780	6	79	theme	optimized	922:930	arg1	response					932:939	The optimized response	918:939	The optimized response (adsorption capacity)	918:961	The optimized response (adsorption capacity) was reached 62.16 mg g-1 under the optimal conditions (98.77 mg L-1 of boric acid concentration, pH of 5.46 and 76 min).
34648780	6	79	theme	optimized	922:930	arg1	capacity					953:960	adsorption capacity	942:960	adsorption capacity	942:960	The optimized response (adsorption capacity) was reached 62.16 mg g-1 under the optimal conditions (98.77 mg L-1 of boric acid concentration, pH of 5.46 and 76 min).
34648780	4	80	theme	optimization	623:634	arg1	problem					636:642	the constrained optimization problem	607:642	the constrained optimization problem with an engineering approach	607:671	Moreover, this study has related to the constrained optimization problem with an engineering approach.
34648780	10	81	theme	boric	1579:1583	arg1	acid					1585:1588	boric acid	1579:1588	boric acid concentration	1579:1602	The optimal conditions derived from SA were 91.17 mg L-1 of boric acid concentration, pH of 5.86, and 76.17 min.
34648780	5	82	with	method	691:696	arg1	FCCCD					749:753	FCCCD	749:753	FCCCD	749:753	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	5	82	with	method	691:696	arg1	design					741:746	face-centered central composite design	709:746	face-centered central composite design (FCCCD)	709:754	Response surface method (RSM) with face-centered central composite design (FCCCD) was chosen for maximizing the adsorption capacity as well as determining optimal independent factors such as pH, adsorbent dose, and concentration of boric acid.
34648780	1	83	theme	major	265:269	arg1	contamination					176:188	Boron contamination	170:188	Boron contamination in water resources (especially drinking waters and agricultural land)	170:258	Boron contamination in water resources (especially drinking waters and agricultural land) is a major problem for the ecosystem.
34648780	1	83	theme	major	265:269	arg1	problem					271:277	a major problem	263:277	a major problem for the ecosystem	263:295	Boron contamination in water resources (especially drinking waters and agricultural land) is a major problem for the ecosystem.
34176028	5	0	theme	NST	1021:1023	arg1	yield					1045:1049	the yield	1041:1049	the yield of heparosan	1041:1062	The fed-batch fermentation of EcN::T7M carrying the plasmid expressing NST was carried out, which brought the yield of NST to 0.21 g/L and the yield of heparosan to 0.85 g/L, respectively.
34176028	5	0	theme	NST	1021:1023	arg1	yield					1012:1016	the yield	1008:1016	the yield of NST to 0.21 g/L	1008:1035	The fed-batch fermentation of EcN::T7M carrying the plasmid expressing NST was carried out, which brought the yield of NST to 0.21 g/L and the yield of heparosan to 0.85 g/L, respectively.
34176028	4	1	from	3-O-sulfotransferase-1	857:878	arg1	flask					895:899	a flask	893:899	a flask	893:899	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	2	theme	mouse	851:855	arg1	3-OST-1					881:887	3-OST-1	881:887	3-OST-1	881:887	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	2	theme	mouse	851:855	arg1	3-O-sulfotransferase-1					857:878	mouse 3-O-sulfotransferase-1	851:878	mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask	851:899	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	7	3	theme	repeats	1350:1356	arg1	%					1332:1332	over 80%	1325:1332	over 80% of disaccharide repeats of heparosan	1325:1369	The analysis of element composition showed that over 80% of disaccharide repeats of heparosan were N-sulfated.
34176028	7	3	theme	repeats	1350:1356	arg1	repeats					1350:1356	disaccharide repeats	1337:1356	disaccharide repeats of heparosan	1337:1369	The analysis of element composition showed that over 80% of disaccharide repeats of heparosan were N-sulfated.
34176028	8	4	theme	::	1431:1432	arg1	T7M					1433:1435	EcN::T7M	1428:1435	EcN::T7M	1428:1435	CONCLUSIONS These results indicate that EcN::T7M is capable of preparing sulfotransferase and heparosan at the same time.
34176028	4	5	theme	BL21	688:691	arg1	region					678:683	the λDE3 region	669:683	the λDE3 region of BL21(DE3) encoding T7 RNA polymerase	669:723	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	9	6	theme	::	1517:1518	arg1	host					1549:1552	a suitable host	1538:1552	a suitable host for expressing exogenous proteins driven by tac promoter and T7 promoter	1538:1625	The EcN::T7M strain is also a suitable host for expressing exogenous proteins driven by tac promoter and T7 promoter.
34176028	9	6	theme	::	1517:1518	arg1	strain					1523:1528	The EcN::T7M strain	1510:1528	The EcN::T7M strain	1510:1528	The EcN::T7M strain is also a suitable host for expressing exogenous proteins driven by tac promoter and T7 promoter.
34176028	6	7	theme	nuclear	1152:1158	arg1	NMR					1180:1182	NMR	1180:1182	NMR	1180:1182	Furthermore, the heparosan was purified, characterized by 1H nuclear magnetic resonance (NMR), and sulfated by NST using 3'-phosphoadenosine-5'-phosphosulfate (PAPS) as the sulfo donor.
34176028	6	7	theme	nuclear	1152:1158	arg1	resonance					1169:1177	1H nuclear magnetic resonance	1149:1177	1H nuclear magnetic resonance (NMR)	1149:1183	Furthermore, the heparosan was purified, characterized by 1H nuclear magnetic resonance (NMR), and sulfated by NST using 3'-phosphoadenosine-5'-phosphosulfate (PAPS) as the sulfo donor.
34176028	4	8	from	domain	757:762	arg1	flask					895:899	a flask	893:899	a flask	893:899	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	9	theme	sulfotransferase	740:755	arg1	3-O-sulfotransferase-1					857:878	mouse 3-O-sulfotransferase-1	851:878	mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask	851:899	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	9	theme	sulfotransferase	740:755	arg1	NST					765:767	NST	765:767	NST	765:767	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	9	theme	sulfotransferase	740:755	arg1	domain					757:762	the sulfotransferase domain	736:762	the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1)	736:821	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	9	theme	sulfotransferase	740:755	arg1	N-deacetylase/N-sulfotransferase-1					779:812	human N-deacetylase/N-sulfotransferase-1	773:812	human N-deacetylase/N-sulfotransferase-1 (NDST-1)	773:821	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	5	10	theme	::	935:936	arg1	T7M					937:939	EcN::T7M	932:939	EcN::T7M carrying the plasmid expressing NST	932:975	The fed-batch fermentation of EcN::T7M carrying the plasmid expressing NST was carried out, which brought the yield of NST to 0.21 g/L and the yield of heparosan to 0.85 g/L, respectively.
34176028	4	11	from	domain	841:846	arg1	flask					895:899	a flask	893:899	a flask	893:899	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	12	theme	3-O-sulfotransferase-1	857:878	arg1	3-O-sulfotransferase-1					857:878	mouse 3-O-sulfotransferase-1	851:878	mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask	851:899	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	12	theme	3-O-sulfotransferase-1	857:878	arg1	NST					765:767	NST	765:767	NST	765:767	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	12	theme	3-O-sulfotransferase-1	857:878	arg1	domain					841:846	the catalytic domain	827:846	the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask	827:899	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	12	theme	3-O-sulfotransferase-1	857:878	arg1	domain					757:762	the sulfotransferase domain	736:762	the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1)	736:821	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	12	theme	3-O-sulfotransferase-1	857:878	arg1	N-deacetylase/N-sulfotransferase-1					779:812	human N-deacetylase/N-sulfotransferase-1	773:812	human N-deacetylase/N-sulfotransferase-1 (NDST-1)	773:821	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	5	13	theme	fed-batch	906:914	arg1	fermentation					916:927	The fed-batch fermentation	902:927	The fed-batch fermentation of EcN::T7M carrying the plasmid expressing NST	902:975	The fed-batch fermentation of EcN::T7M carrying the plasmid expressing NST was carried out, which brought the yield of NST to 0.21 g/L and the yield of heparosan to 0.85 g/L, respectively.
34176028	2	14	theme	such	340:343	arg1	material					345:352	such material	340:352	such material	340:352	However, the preparation of heparosan and enzymes was considered tedious since such material must be prepared in separate fermentation batches.
34176028	2	15	theme	heparosan	289:297	arg1	preparation					274:284	the preparation	270:284	the preparation of heparosan and enzymes	270:309	However, the preparation of heparosan and enzymes was considered tedious since such material must be prepared in separate fermentation batches.
34176028	8	16	theme	EcN	1428:1430	arg1	T7M					1433:1435	EcN::T7M	1428:1435	EcN::T7M	1428:1435	CONCLUSIONS These results indicate that EcN::T7M is capable of preparing sulfotransferase and heparosan at the same time.
34176028	5	17	theme	heparosan	1054:1062	arg1	yield					1045:1049	the yield	1041:1049	the yield of heparosan	1041:1062	The fed-batch fermentation of EcN::T7M carrying the plasmid expressing NST was carried out, which brought the yield of NST to 0.21 g/L and the yield of heparosan to 0.85 g/L, respectively.
34176028	5	17	theme	heparosan	1054:1062	arg1	yield					1012:1016	the yield	1008:1016	the yield of NST to 0.21 g/L	1008:1035	The fed-batch fermentation of EcN::T7M carrying the plasmid expressing NST was carried out, which brought the yield of NST to 0.21 g/L and the yield of heparosan to 0.85 g/L, respectively.
34176028	9	18	theme	tac	1598:1600	arg1	promoter					1602:1609	tac promoter	1598:1609	tac promoter	1598:1609	The EcN::T7M strain is also a suitable host for expressing exogenous proteins driven by tac promoter and T7 promoter.
34176028	9	19	theme	suitable	1540:1547	arg1	host					1549:1552	a suitable host	1538:1552	a suitable host for expressing exogenous proteins driven by tac promoter and T7 promoter	1538:1625	The EcN::T7M strain is also a suitable host for expressing exogenous proteins driven by tac promoter and T7 promoter.
34176028	9	19	theme	suitable	1540:1547	arg1	strain					1523:1528	The EcN::T7M strain	1510:1528	The EcN::T7M strain	1510:1528	The EcN::T7M strain is also a suitable host for expressing exogenous proteins driven by tac promoter and T7 promoter.
34176028	7	20	theme	heparosan	1361:1369	arg1	repeats					1350:1356	disaccharide repeats	1337:1356	disaccharide repeats of heparosan	1337:1369	The analysis of element composition showed that over 80% of disaccharide repeats of heparosan were N-sulfated.
34176028	5	21	contain	carrying	941:948	arg2	plasmid					954:960	the plasmid	950:960	the plasmid expressing NST	950:975	The fed-batch fermentation of EcN::T7M carrying the plasmid expressing NST was carried out, which brought the yield of NST to 0.21 g/L and the yield of heparosan to 0.85 g/L, respectively.
34176028	5	21	contain	carrying	941:948	arg1	T7M					937:939	EcN::T7M	932:939	EcN::T7M carrying the plasmid expressing NST	932:975	The fed-batch fermentation of EcN::T7M carrying the plasmid expressing NST was carried out, which brought the yield of NST to 0.21 g/L and the yield of heparosan to 0.85 g/L, respectively.
34176028	3	22	theme	Escherichia	451:461	arg1	EcN					488:490	EcN	488:490	EcN	488:490	In this study, a commonly admitted probiotic, Escherichia coli strain Nissle 1917 (EcN), was engineered to intracellularly express sulfotransferases and, simultaneously, secreting heparosan into the culture medium.
34176028	3	22	theme	Escherichia	451:461	arg1	probiotic					440:448	probiotic	440:448	probiotic	440:448	In this study, a commonly admitted probiotic, Escherichia coli strain Nissle 1917 (EcN), was engineered to intracellularly express sulfotransferases and, simultaneously, secreting heparosan into the culture medium.
34176028	3	22	theme	Escherichia	451:461	arg1	1917					482:485	Escherichia coli strain Nissle 1917	451:485	Escherichia coli strain Nissle 1917 (EcN)	451:491	In this study, a commonly admitted probiotic, Escherichia coli strain Nissle 1917 (EcN), was engineered to intracellularly express sulfotransferases and, simultaneously, secreting heparosan into the culture medium.
34176028	4	23	theme	::	653:654	arg1	T7M					655:657	The engineered strain EcN::T7M	628:657	RESULTS The engineered strain EcN::T7M	620:657	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	7	24	theme	disaccharide	1337:1348	arg1	repeats					1350:1356	disaccharide repeats	1337:1356	disaccharide repeats of heparosan	1337:1369	The analysis of element composition showed that over 80% of disaccharide repeats of heparosan were N-sulfated.
34176028	4	25	theme	λDE3	673:676	arg1	region					678:683	the λDE3 region	669:683	the λDE3 region of BL21(DE3) encoding T7 RNA polymerase	669:723	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	26	theme	catalytic	831:839	arg1	domain					841:846	the catalytic domain	827:846	the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask	827:899	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	26	theme	catalytic	831:839	arg1	3-O-sulfotransferase-1					857:878	mouse 3-O-sulfotransferase-1	851:878	mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask	851:899	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	26	theme	catalytic	831:839	arg1	N-deacetylase/N-sulfotransferase-1					779:812	human N-deacetylase/N-sulfotransferase-1	773:812	human N-deacetylase/N-sulfotransferase-1 (NDST-1)	773:821	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	1	27	theme	OBJECTIVES	103:112	arg1	Heparosan					114:122	OBJECTIVES Heparosan	103:122	OBJECTIVES Heparosan	103:122	OBJECTIVES Heparosan is used as the starting polysaccharide sulfated using sulfotransferase to generate fully elaborate heparin, a widely used clinical drug.
34176028	1	27	theme	OBJECTIVES	103:112	arg1	polysaccharide					148:161	the starting polysaccharide	135:161	the starting polysaccharide sulfated using sulfotransferase to generate fully elaborate heparin, a widely used clinical drug	135:258	OBJECTIVES Heparosan is used as the starting polysaccharide sulfated using sulfotransferase to generate fully elaborate heparin, a widely used clinical drug.
34176028	7	28	theme	composition	1301:1311	arg1	analysis					1281:1288	The analysis	1277:1288	The analysis of element composition	1277:1311	The analysis of element composition showed that over 80% of disaccharide repeats of heparosan were N-sulfated.
34176028	9	29	theme	exogenous	1569:1577	arg1	proteins					1579:1586	exogenous proteins	1569:1586	exogenous proteins driven by tac promoter and T7 promoter	1569:1625	The EcN::T7M strain is also a suitable host for expressing exogenous proteins driven by tac promoter and T7 promoter.
34176028	6	30	theme	1H	1149:1150	arg1	NMR					1180:1182	NMR	1180:1182	NMR	1180:1182	Furthermore, the heparosan was purified, characterized by 1H nuclear magnetic resonance (NMR), and sulfated by NST using 3'-phosphoadenosine-5'-phosphosulfate (PAPS) as the sulfo donor.
34176028	6	30	theme	1H	1149:1150	arg1	resonance					1169:1177	1H nuclear magnetic resonance	1149:1177	1H nuclear magnetic resonance (NMR)	1149:1183	Furthermore, the heparosan was purified, characterized by 1H nuclear magnetic resonance (NMR), and sulfated by NST using 3'-phosphoadenosine-5'-phosphosulfate (PAPS) as the sulfo donor.
34176028	9	31	theme	T7	1615:1616	arg1	promoter					1618:1625	T7 promoter	1615:1625	T7 promoter	1615:1625	The EcN::T7M strain is also a suitable host for expressing exogenous proteins driven by tac promoter and T7 promoter.
34176028	1	32	dep	generate	198:205	arg1	elaborate					213:221	elaborate	213:221	to generate fully elaborate heparin, a widely used clinical drug	195:258	OBJECTIVES Heparosan is used as the starting polysaccharide sulfated using sulfotransferase to generate fully elaborate heparin, a widely used clinical drug.
34176028	5	33	theme	EcN	932:934	arg1	T7M					937:939	EcN::T7M	932:939	EcN::T7M carrying the plasmid expressing NST	932:975	The fed-batch fermentation of EcN::T7M carrying the plasmid expressing NST was carried out, which brought the yield of NST to 0.21 g/L and the yield of heparosan to 0.85 g/L, respectively.
34176028	9	34	theme	T7M	1519:1521	arg1	host					1549:1552	a suitable host	1538:1552	a suitable host for expressing exogenous proteins driven by tac promoter and T7 promoter	1538:1625	The EcN::T7M strain is also a suitable host for expressing exogenous proteins driven by tac promoter and T7 promoter.
34176028	9	34	theme	T7M	1519:1521	arg1	strain					1523:1528	The EcN::T7M strain	1510:1528	The EcN::T7M strain	1510:1528	The EcN::T7M strain is also a suitable host for expressing exogenous proteins driven by tac promoter and T7 promoter.
34176028	3	35	theme	Nissle	475:480	arg1	EcN					488:490	EcN	488:490	EcN	488:490	In this study, a commonly admitted probiotic, Escherichia coli strain Nissle 1917 (EcN), was engineered to intracellularly express sulfotransferases and, simultaneously, secreting heparosan into the culture medium.
34176028	3	35	theme	Nissle	475:480	arg1	probiotic					440:448	probiotic	440:448	probiotic	440:448	In this study, a commonly admitted probiotic, Escherichia coli strain Nissle 1917 (EcN), was engineered to intracellularly express sulfotransferases and, simultaneously, secreting heparosan into the culture medium.
34176028	3	35	theme	Nissle	475:480	arg1	1917					482:485	Escherichia coli strain Nissle 1917	451:485	Escherichia coli strain Nissle 1917 (EcN)	451:491	In this study, a commonly admitted probiotic, Escherichia coli strain Nissle 1917 (EcN), was engineered to intracellularly express sulfotransferases and, simultaneously, secreting heparosan into the culture medium.
34176028	4	36	theme	RNA	710:712	arg1	polymerase					714:723	T7 RNA polymerase	707:723	T7 RNA polymerase	707:723	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	1	37	used	used	127:130	arg2	Heparosan					114:122	OBJECTIVES Heparosan	103:122	OBJECTIVES Heparosan	103:122	OBJECTIVES Heparosan is used as the starting polysaccharide sulfated using sulfotransferase to generate fully elaborate heparin, a widely used clinical drug.
34176028	1	37	used	used	127:130	arg2	polysaccharide					148:161	the starting polysaccharide	135:161	the starting polysaccharide sulfated using sulfotransferase to generate fully elaborate heparin, a widely used clinical drug	135:258	OBJECTIVES Heparosan is used as the starting polysaccharide sulfated using sulfotransferase to generate fully elaborate heparin, a widely used clinical drug.
34176028	4	38	theme	strain	643:648	arg1	T7M					655:657	The engineered strain EcN::T7M	628:657	RESULTS The engineered strain EcN::T7M	620:657	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	0	39	theme	strain	38:43	arg1	construction					4:15	The construction	0:15	The construction of a dual-functional strain that produces both polysaccharides and sulfotransferases	0:100	The construction of a dual-functional strain that produces both polysaccharides and sulfotransferases.
34176028	5	40	theme	T7M	937:939	arg1	fermentation					916:927	The fed-batch fermentation	902:927	The fed-batch fermentation of EcN::T7M carrying the plasmid expressing NST	902:975	The fed-batch fermentation of EcN::T7M carrying the plasmid expressing NST was carried out, which brought the yield of NST to 0.21 g/L and the yield of heparosan to 0.85 g/L, respectively.
34176028	3	41	theme	culture	604:610	arg1	medium					612:617	the culture medium	600:617	the culture medium	600:617	In this study, a commonly admitted probiotic, Escherichia coli strain Nissle 1917 (EcN), was engineered to intracellularly express sulfotransferases and, simultaneously, secreting heparosan into the culture medium.
34176028	0	42	theme	dual-functional	22:36	arg1	strain					38:43	a dual-functional strain	20:43	a dual-functional strain that produces both polysaccharides and sulfotransferases	20:100	The construction of a dual-functional strain that produces both polysaccharides and sulfotransferases.
34176028	6	43	theme	sulfo	1264:1268	arg1	donor					1270:1274	the sulfo donor	1260:1274	the sulfo donor	1260:1274	Furthermore, the heparosan was purified, characterized by 1H nuclear magnetic resonance (NMR), and sulfated by NST using 3'-phosphoadenosine-5'-phosphosulfate (PAPS) as the sulfo donor.
34176028	6	43	theme	sulfo	1264:1268	arg1	3'-phosphoadenosine-5'-phosphosulfate					1212:1248	3'-phosphoadenosine-5'-phosphosulfate	1212:1248	3'-phosphoadenosine-5'-phosphosulfate (PAPS)	1212:1255	Furthermore, the heparosan was purified, characterized by 1H nuclear magnetic resonance (NMR), and sulfated by NST using 3'-phosphoadenosine-5'-phosphosulfate (PAPS) as the sulfo donor.
34176028	4	44	theme	N-deacetylase/N-sulfotransferase-1	779:812	arg1	3-O-sulfotransferase-1					857:878	mouse 3-O-sulfotransferase-1	851:878	mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask	851:899	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	44	theme	N-deacetylase/N-sulfotransferase-1	779:812	arg1	NST					765:767	NST	765:767	NST	765:767	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	44	theme	N-deacetylase/N-sulfotransferase-1	779:812	arg1	domain					841:846	the catalytic domain	827:846	the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask	827:899	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	44	theme	N-deacetylase/N-sulfotransferase-1	779:812	arg1	domain					757:762	the sulfotransferase domain	736:762	the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1)	736:821	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	44	theme	N-deacetylase/N-sulfotransferase-1	779:812	arg1	N-deacetylase/N-sulfotransferase-1					779:812	human N-deacetylase/N-sulfotransferase-1	773:812	human N-deacetylase/N-sulfotransferase-1 (NDST-1)	773:821	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	3	45	theme	strain	468:473	arg1	EcN					488:490	EcN	488:490	EcN	488:490	In this study, a commonly admitted probiotic, Escherichia coli strain Nissle 1917 (EcN), was engineered to intracellularly express sulfotransferases and, simultaneously, secreting heparosan into the culture medium.
34176028	3	45	theme	strain	468:473	arg1	probiotic					440:448	probiotic	440:448	probiotic	440:448	In this study, a commonly admitted probiotic, Escherichia coli strain Nissle 1917 (EcN), was engineered to intracellularly express sulfotransferases and, simultaneously, secreting heparosan into the culture medium.
34176028	3	45	theme	strain	468:473	arg1	1917					482:485	Escherichia coli strain Nissle 1917	451:485	Escherichia coli strain Nissle 1917 (EcN)	451:491	In this study, a commonly admitted probiotic, Escherichia coli strain Nissle 1917 (EcN), was engineered to intracellularly express sulfotransferases and, simultaneously, secreting heparosan into the culture medium.
34176028	4	46	from	flask	895:899	arg1	3-O-sulfotransferase-1					857:878	mouse 3-O-sulfotransferase-1	851:878	mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask	851:899	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	46	from	flask	895:899	arg1	NST					765:767	NST	765:767	NST	765:767	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	46	from	flask	895:899	arg1	domain					841:846	the catalytic domain	827:846	the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask	827:899	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	46	from	flask	895:899	arg1	domain					757:762	the sulfotransferase domain	736:762	the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1)	736:821	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	46	from	flask	895:899	arg1	N-deacetylase/N-sulfotransferase-1					779:812	human N-deacetylase/N-sulfotransferase-1	773:812	human N-deacetylase/N-sulfotransferase-1 (NDST-1)	773:821	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	3	47	dep	express	528:534	arg1	secreting					575:583	secreting	575:583	secreting heparosan into the culture medium	575:617	In this study, a commonly admitted probiotic, Escherichia coli strain Nissle 1917 (EcN), was engineered to intracellularly express sulfotransferases and, simultaneously, secreting heparosan into the culture medium.
34176028	8	48	dep	CONCLUSIONS	1388:1398	arg1	indicate					1414:1421	indicate	1414:1421	indicate that EcN::T7M is capable of preparing sulfotransferase and heparosan at the same time	1414:1507	CONCLUSIONS These results indicate that EcN::T7M is capable of preparing sulfotransferase and heparosan at the same time.
34176028	8	49	theme	same	1499:1502	arg1	time					1504:1507	the same time	1495:1507	the same time	1495:1507	CONCLUSIONS These results indicate that EcN::T7M is capable of preparing sulfotransferase and heparosan at the same time.
34176028	3	50	dep	Escherichia	451:461	arg1	coli					463:466	coli	463:466	coli	463:466	In this study, a commonly admitted probiotic, Escherichia coli strain Nissle 1917 (EcN), was engineered to intracellularly express sulfotransferases and, simultaneously, secreting heparosan into the culture medium.
34176028	7	51	theme	element	1293:1299	arg1	composition					1301:1311	element composition	1293:1311	element composition	1293:1311	The analysis of element composition showed that over 80% of disaccharide repeats of heparosan were N-sulfated.
34176028	6	52	theme	magnetic	1160:1167	arg1	NMR					1180:1182	NMR	1180:1182	NMR	1180:1182	Furthermore, the heparosan was purified, characterized by 1H nuclear magnetic resonance (NMR), and sulfated by NST using 3'-phosphoadenosine-5'-phosphosulfate (PAPS) as the sulfo donor.
34176028	6	52	theme	magnetic	1160:1167	arg1	resonance					1169:1177	1H nuclear magnetic resonance	1149:1177	1H nuclear magnetic resonance (NMR)	1149:1183	Furthermore, the heparosan was purified, characterized by 1H nuclear magnetic resonance (NMR), and sulfated by NST using 3'-phosphoadenosine-5'-phosphosulfate (PAPS) as the sulfo donor.
34176028	4	53	theme	T7	707:708	arg1	polymerase					714:723	T7 RNA polymerase	707:723	T7 RNA polymerase	707:723	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	9	54	theme	EcN	1514:1516	arg1	host					1549:1552	a suitable host	1538:1552	a suitable host for expressing exogenous proteins driven by tac promoter and T7 promoter	1538:1625	The EcN::T7M strain is also a suitable host for expressing exogenous proteins driven by tac promoter and T7 promoter.
34176028	9	54	theme	EcN	1514:1516	arg1	strain					1523:1528	The EcN::T7M strain	1510:1528	The EcN::T7M strain	1510:1528	The EcN::T7M strain is also a suitable host for expressing exogenous proteins driven by tac promoter and T7 promoter.
34176028	1	55	theme	used	241:244	arg1	drug					255:258	a widely used clinical drug	232:258	a widely used clinical drug	232:258	OBJECTIVES Heparosan is used as the starting polysaccharide sulfated using sulfotransferase to generate fully elaborate heparin, a widely used clinical drug.
34176028	1	55	theme	used	241:244	arg1	heparin					223:229	heparin	223:229	heparin	223:229	OBJECTIVES Heparosan is used as the starting polysaccharide sulfated using sulfotransferase to generate fully elaborate heparin, a widely used clinical drug.
34176028	4	56	dep	RESULTS	620:626	arg1	T7M					655:657	The engineered strain EcN::T7M	628:657	RESULTS The engineered strain EcN::T7M	620:657	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	2	57	theme	enzymes	303:309	arg1	preparation					274:284	the preparation	270:284	the preparation of heparosan and enzymes	270:309	However, the preparation of heparosan and enzymes was considered tedious since such material must be prepared in separate fermentation batches.
34176028	1	58	theme	starting	139:146	arg1	Heparosan					114:122	OBJECTIVES Heparosan	103:122	OBJECTIVES Heparosan	103:122	OBJECTIVES Heparosan is used as the starting polysaccharide sulfated using sulfotransferase to generate fully elaborate heparin, a widely used clinical drug.
34176028	1	58	theme	starting	139:146	arg1	polysaccharide					148:161	the starting polysaccharide	135:161	the starting polysaccharide sulfated using sulfotransferase to generate fully elaborate heparin, a widely used clinical drug	135:258	OBJECTIVES Heparosan is used as the starting polysaccharide sulfated using sulfotransferase to generate fully elaborate heparin, a widely used clinical drug.
34176028	1	59	theme	clinical	246:253	arg1	drug					255:258	a widely used clinical drug	232:258	a widely used clinical drug	232:258	OBJECTIVES Heparosan is used as the starting polysaccharide sulfated using sulfotransferase to generate fully elaborate heparin, a widely used clinical drug.
34176028	1	59	theme	clinical	246:253	arg1	heparin					223:229	heparin	223:229	heparin	223:229	OBJECTIVES Heparosan is used as the starting polysaccharide sulfated using sulfotransferase to generate fully elaborate heparin, a widely used clinical drug.
34176028	4	60	contain	carrying	660:667	arg1	RESULTS					620:626	RESULTS The engineered strain EcN::T7M	620:657	RESULTS The engineered strain EcN::T7M	620:657	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	60	contain	carrying	660:667	arg2	region					678:683	the λDE3 region	669:683	the λDE3 region of BL21(DE3) encoding T7 RNA polymerase	669:723	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	61	theme	human	773:777	arg1	NDST-1					815:820	NDST-1	815:820	NDST-1	815:820	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	61	theme	human	773:777	arg1	N-deacetylase/N-sulfotransferase-1					779:812	human N-deacetylase/N-sulfotransferase-1	773:812	human N-deacetylase/N-sulfotransferase-1 (NDST-1)	773:821	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	4	62	theme	EcN	650:652	arg1	T7M					655:657	The engineered strain EcN::T7M	628:657	RESULTS The engineered strain EcN::T7M	620:657	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	2	63	theme	fermentation	383:394	arg1	batches					396:402	separate fermentation batches	374:402	separate fermentation batches	374:402	However, the preparation of heparosan and enzymes was considered tedious since such material must be prepared in separate fermentation batches.
34176028	4	64	theme	engineered	632:641	arg1	T7M					655:657	The engineered strain EcN::T7M	628:657	RESULTS The engineered strain EcN::T7M	620:657	RESULTS The engineered strain EcN::T7M, carrying the λDE3 region of BL21(DE3) encoding T7 RNA polymerase, expressed the sulfotransferase domain (NST) of human N-deacetylase/N-sulfotransferase-1 (NDST-1) and the catalytic domain of mouse 3-O-sulfotransferase-1 (3-OST-1) in a flask.
34176028	2	65	theme	separate	374:381	arg1	batches					396:402	separate fermentation batches	374:402	separate fermentation batches	374:402	However, the preparation of heparosan and enzymes was considered tedious since such material must be prepared in separate fermentation batches.
33620143	5	0	theme	pre-clinical	517:528	arg1	elegans					551:557	the pre-clinical model Caenorhabditis elegans	513:557	the pre-clinical model Caenorhabditis elegans	513:557	Analyzing the basis of this observation and using the pre-clinical model Caenorhabditis elegans, we have found that lipoteichoic acid (LTA) of BPL1 is responsible for its fat-reducing properties and that this attribute is preserved under hyperglycaemic conditions.
33620143	6	1	theme	chemical	851:858	arg1	composition					860:870	LTA chemical composition	847:870	LTA chemical composition	847:870	This fat-reducing capacity of both BPL1 and LTA-BPL1 is abolished under glucose restriction, as a result of changes in LTA chemical composition.
33620143	8	2	theme	beneficial	1059:1068	arg1	properties					1070:1079	beneficial properties	1059:1079	beneficial properties	1059:1079	These results open the possibility of using LTA as a novel postbiotic, whose beneficial properties can be applied therapeutically and/or preventively in metabolic syndrome and diabetes-related disorders.
33620143	4	3	theme	lactis	391:396	arg1	CECT8145					404:411	CECT8145	404:411	CECT8145	404:411	lactis BPL1 (CECT8145) has been shown to ameliorate obesity biomarkers.
33620143	4	3	theme	lactis	391:396	arg1	BPL1					398:401	lactis BPL1	391:401	lactis BPL1 (CECT8145)	391:412	lactis BPL1 (CECT8145) has been shown to ameliorate obesity biomarkers.
33620143	6	4	theme	changes	836:842	arg1	capacity					746:753	This fat-reducing capacity	728:753	This fat-reducing capacity of both BPL1 and LTA-BPL1	728:779	This fat-reducing capacity of both BPL1 and LTA-BPL1 is abolished under glucose restriction, as a result of changes in LTA chemical composition.
33620143	6	4	theme	changes	836:842	arg1	result					826:831	a result	824:831	a result of changes in LTA chemical composition	824:870	This fat-reducing capacity of both BPL1 and LTA-BPL1 is abolished under glucose restriction, as a result of changes in LTA chemical composition.
33620143	5	5	theme	hyperglycaemic	701:714	arg1	conditions					716:725	hyperglycaemic conditions	701:725	hyperglycaemic conditions	701:725	Analyzing the basis of this observation and using the pre-clinical model Caenorhabditis elegans, we have found that lipoteichoic acid (LTA) of BPL1 is responsible for its fat-reducing properties and that this attribute is preserved under hyperglycaemic conditions.
33620143	7	6	theme	IGF-1	946:950	arg1	pathway					952:958	the IGF-1 pathway	942:958	the IGF-1 pathway	942:958	Moreover, we have demonstrated that LTA exerts this function through the IGF-1 pathway, as does BPL1 strain.
33620143	6	7	theme	glucose	800:806	arg1	restriction					808:818	glucose restriction	800:818	glucose restriction	800:818	This fat-reducing capacity of both BPL1 and LTA-BPL1 is abolished under glucose restriction, as a result of changes in LTA chemical composition.
33620143	3	8	theme	probiotic	342:350	arg1	subsp					384:388	the probiotic strain Bifidobacterium animalis subsp	338:388	the probiotic strain Bifidobacterium animalis subsp	338:388	In this context, supplementation with the probiotic strain Bifidobacterium animalis subsp.
33620143	5	9	theme	fat-reducing	634:645	arg1	properties					647:656	its fat-reducing properties	630:656	its fat-reducing properties	630:656	Analyzing the basis of this observation and using the pre-clinical model Caenorhabditis elegans, we have found that lipoteichoic acid (LTA) of BPL1 is responsible for its fat-reducing properties and that this attribute is preserved under hyperglycaemic conditions.
33620143	8	10	theme	metabolic	1135:1143	arg1	syndrome					1145:1152	metabolic syndrome	1135:1152	metabolic syndrome	1135:1152	These results open the possibility of using LTA as a novel postbiotic, whose beneficial properties can be applied therapeutically and/or preventively in metabolic syndrome and diabetes-related disorders.
33620143	1	11	theme	novel	70:74	arg1	postbiotic					76:85	a novel postbiotic	68:85	lactis BPL1: a novel postbiotic that reduces fat deposition via IGF-1 pathway.	55:132	lactis BPL1: a novel postbiotic that reduces fat deposition via IGF-1 pathway.
33620143	3	12	theme	animalis	375:382	arg1	subsp					384:388	the probiotic strain Bifidobacterium animalis subsp	338:388	the probiotic strain Bifidobacterium animalis subsp	338:388	In this context, supplementation with the probiotic strain Bifidobacterium animalis subsp.
33620143	0	13	from	animalis	39:46	arg1	acid					13:16	Lipoteichoic acid	0:16	Lipoteichoic acid from Bifidobacterium animalis	0:46	Lipoteichoic acid from Bifidobacterium animalis subsp.
33620143	5	14	theme	BPL1	606:609	arg1	acid					592:595	lipoteichoic acid	579:595	lipoteichoic acid (LTA) of BPL1	579:609	Analyzing the basis of this observation and using the pre-clinical model Caenorhabditis elegans, we have found that lipoteichoic acid (LTA) of BPL1 is responsible for its fat-reducing properties and that this attribute is preserved under hyperglycaemic conditions.
33620143	5	14	theme	BPL1	606:609	arg1	LTA					598:600	LTA	598:600	LTA	598:600	Analyzing the basis of this observation and using the pre-clinical model Caenorhabditis elegans, we have found that lipoteichoic acid (LTA) of BPL1 is responsible for its fat-reducing properties and that this attribute is preserved under hyperglycaemic conditions.
33620143	6	15	theme	LTA-BPL1	772:779	arg1	capacity					746:753	This fat-reducing capacity	728:753	This fat-reducing capacity of both BPL1 and LTA-BPL1	728:779	This fat-reducing capacity of both BPL1 and LTA-BPL1 is abolished under glucose restriction, as a result of changes in LTA chemical composition.
33620143	6	15	theme	LTA-BPL1	772:779	arg1	result					826:831	a result	824:831	a result of changes in LTA chemical composition	824:870	This fat-reducing capacity of both BPL1 and LTA-BPL1 is abolished under glucose restriction, as a result of changes in LTA chemical composition.
33620143	5	16	theme	observation	491:501	arg1	basis					477:481	the basis	473:481	the basis of this observation	473:501	Analyzing the basis of this observation and using the pre-clinical model Caenorhabditis elegans, we have found that lipoteichoic acid (LTA) of BPL1 is responsible for its fat-reducing properties and that this attribute is preserved under hyperglycaemic conditions.
33620143	0	17	theme	Lipoteichoic	0:11	arg1	acid					13:16	Lipoteichoic acid	0:16	Lipoteichoic acid from Bifidobacterium animalis	0:46	Lipoteichoic acid from Bifidobacterium animalis subsp.
33620143	3	18	theme	strain	352:357	arg1	subsp					384:388	the probiotic strain Bifidobacterium animalis subsp	338:388	the probiotic strain Bifidobacterium animalis subsp	338:388	In this context, supplementation with the probiotic strain Bifidobacterium animalis subsp.
33620143	8	19	theme	novel	1035:1039	arg1	postbiotic					1041:1050	a novel postbiotic	1033:1050	a novel postbiotic	1033:1050	These results open the possibility of using LTA as a novel postbiotic, whose beneficial properties can be applied therapeutically and/or preventively in metabolic syndrome and diabetes-related disorders.
33620143	8	19	theme	novel	1035:1039	arg1	LTA					1026:1028	LTA	1026:1028	LTA	1026:1028	These results open the possibility of using LTA as a novel postbiotic, whose beneficial properties can be applied therapeutically and/or preventively in metabolic syndrome and diabetes-related disorders.
33620143	6	20	theme	BPL1	763:766	arg1	capacity					746:753	This fat-reducing capacity	728:753	This fat-reducing capacity of both BPL1 and LTA-BPL1	728:779	This fat-reducing capacity of both BPL1 and LTA-BPL1 is abolished under glucose restriction, as a result of changes in LTA chemical composition.
33620143	6	20	theme	BPL1	763:766	arg1	result					826:831	a result	824:831	a result of changes in LTA chemical composition	824:870	This fat-reducing capacity of both BPL1 and LTA-BPL1 is abolished under glucose restriction, as a result of changes in LTA chemical composition.
33620143	2	21	theme	world	282:286	arg1	population					288:297	the world population	278:297	the world population	278:297	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	5	22	theme	lipoteichoic	579:590	arg1	acid					592:595	lipoteichoic acid	579:595	lipoteichoic acid (LTA) of BPL1	579:609	Analyzing the basis of this observation and using the pre-clinical model Caenorhabditis elegans, we have found that lipoteichoic acid (LTA) of BPL1 is responsible for its fat-reducing properties and that this attribute is preserved under hyperglycaemic conditions.
33620143	5	22	theme	lipoteichoic	579:590	arg1	LTA					598:600	LTA	598:600	LTA	598:600	Analyzing the basis of this observation and using the pre-clinical model Caenorhabditis elegans, we have found that lipoteichoic acid (LTA) of BPL1 is responsible for its fat-reducing properties and that this attribute is preserved under hyperglycaemic conditions.
33620143	3	23	theme	Bifidobacterium	359:373	arg1	subsp					384:388	the probiotic strain Bifidobacterium animalis subsp	338:388	the probiotic strain Bifidobacterium animalis subsp	338:388	In this context, supplementation with the probiotic strain Bifidobacterium animalis subsp.
33620143	6	24	theme	LTA	847:849	arg1	composition					860:870	LTA chemical composition	847:870	LTA chemical composition	847:870	This fat-reducing capacity of both BPL1 and LTA-BPL1 is abolished under glucose restriction, as a result of changes in LTA chemical composition.
33620143	1	25	theme	fat	100:102	arg1	deposition					104:113	fat deposition	100:113	fat deposition	100:113	lactis BPL1: a novel postbiotic that reduces fat deposition via IGF-1 pathway.
33620143	3	26	with	supplementation	317:331	arg1	subsp					384:388	the probiotic strain Bifidobacterium animalis subsp	338:388	the probiotic strain Bifidobacterium animalis subsp	338:388	In this context, supplementation with the probiotic strain Bifidobacterium animalis subsp.
33620143	7	27	dep	does	964:967	arg1	as					961:962	as	961:962	as	961:962	Moreover, we have demonstrated that LTA exerts this function through the IGF-1 pathway, as does BPL1 strain.
33620143	4	28	theme	obesity	443:449	arg1	biomarkers					451:460	obesity biomarkers	443:460	obesity biomarkers	443:460	lactis BPL1 (CECT8145) has been shown to ameliorate obesity biomarkers.
33620143	5	29	theme	model	530:534	arg1	elegans					551:557	the pre-clinical model Caenorhabditis elegans	513:557	the pre-clinical model Caenorhabditis elegans	513:557	Analyzing the basis of this observation and using the pre-clinical model Caenorhabditis elegans, we have found that lipoteichoic acid (LTA) of BPL1 is responsible for its fat-reducing properties and that this attribute is preserved under hyperglycaemic conditions.
33620143	8	30	theme	diabetes-related	1158:1173	arg1	disorders					1175:1183	diabetes-related disorders	1158:1183	diabetes-related disorders	1158:1183	These results open the possibility of using LTA as a novel postbiotic, whose beneficial properties can be applied therapeutically and/or preventively in metabolic syndrome and diabetes-related disorders.
33620143	6	31	from	result	826:831	arg1	composition					860:870	LTA chemical composition	847:870	LTA chemical composition	847:870	This fat-reducing capacity of both BPL1 and LTA-BPL1 is abolished under glucose restriction, as a result of changes in LTA chemical composition.
33620143	6	32	from	composition	860:870	arg1	capacity					746:753	This fat-reducing capacity	728:753	This fat-reducing capacity of both BPL1 and LTA-BPL1	728:779	This fat-reducing capacity of both BPL1 and LTA-BPL1 is abolished under glucose restriction, as a result of changes in LTA chemical composition.
33620143	6	32	from	composition	860:870	arg1	result					826:831	a result	824:831	a result of changes in LTA chemical composition	824:870	This fat-reducing capacity of both BPL1 and LTA-BPL1 is abolished under glucose restriction, as a result of changes in LTA chemical composition.
33620143	3	33	dep	supplementation	317:331	arg1	context					308:314	this context	303:314	this context	303:314	In this context, supplementation with the probiotic strain Bifidobacterium animalis subsp.
33620143	2	34	theme	related	150:156	arg1	disorders					168:176	its related metabolic disorders	146:176	its related metabolic disorders	146:176	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	2	34	theme	related	150:156	arg1	Obesity					134:140	Obesity	134:140	Obesity	134:140	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	2	34	theme	related	150:156	arg1	factors					239:245	major risk factors	228:245	major risk factors for morbidity and mortality in the world population	228:297	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	2	34	theme	related	150:156	arg1	diabetes					187:194	diabetes	187:194	diabetes	187:194	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	2	34	theme	related	150:156	arg1	disease					215:221	cardiovascular disease	200:221	cardiovascular disease	200:221	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	5	35	theme	Caenorhabditis	536:549	arg1	elegans					551:557	the pre-clinical model Caenorhabditis elegans	513:557	the pre-clinical model Caenorhabditis elegans	513:557	Analyzing the basis of this observation and using the pre-clinical model Caenorhabditis elegans, we have found that lipoteichoic acid (LTA) of BPL1 is responsible for its fat-reducing properties and that this attribute is preserved under hyperglycaemic conditions.
33620143	2	36	theme	cardiovascular	200:213	arg1	disease					215:221	cardiovascular disease	200:221	cardiovascular disease	200:221	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	7	37	theme	BPL1	969:972	arg1	strain					974:979	BPL1 strain	969:979	BPL1 strain	969:979	Moreover, we have demonstrated that LTA exerts this function through the IGF-1 pathway, as does BPL1 strain.
33620143	2	38	theme	metabolic	158:166	arg1	disorders					168:176	its related metabolic disorders	146:176	its related metabolic disorders	146:176	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	2	38	theme	metabolic	158:166	arg1	Obesity					134:140	Obesity	134:140	Obesity	134:140	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	2	38	theme	metabolic	158:166	arg1	factors					239:245	major risk factors	228:245	major risk factors for morbidity and mortality in the world population	228:297	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	2	38	theme	metabolic	158:166	arg1	diabetes					187:194	diabetes	187:194	diabetes	187:194	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	2	38	theme	metabolic	158:166	arg1	disease					215:221	cardiovascular disease	200:221	cardiovascular disease	200:221	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	1	39	theme	lactis	55:60	arg1	BPL1					62:65	lactis BPL1	55:65	lactis BPL1: a novel postbiotic that reduces fat deposition via IGF-1 pathway.	55:132	lactis BPL1: a novel postbiotic that reduces fat deposition via IGF-1 pathway.
33620143	6	40	theme	fat-reducing	733:744	arg1	capacity					746:753	This fat-reducing capacity	728:753	This fat-reducing capacity of both BPL1 and LTA-BPL1	728:779	This fat-reducing capacity of both BPL1 and LTA-BPL1 is abolished under glucose restriction, as a result of changes in LTA chemical composition.
33620143	6	40	theme	fat-reducing	733:744	arg1	result					826:831	a result	824:831	a result of changes in LTA chemical composition	824:870	This fat-reducing capacity of both BPL1 and LTA-BPL1 is abolished under glucose restriction, as a result of changes in LTA chemical composition.
33620143	1	41	theme	IGF-1	119:123	arg1	pathway					125:131	IGF-1 pathway	119:131	IGF-1 pathway	119:131	lactis BPL1: a novel postbiotic that reduces fat deposition via IGF-1 pathway.
33620143	2	42	from	morbidity	251:259	arg1	population					288:297	the world population	278:297	the world population	278:297	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	1	43	dep	BPL1	62:65	arg1	postbiotic					76:85	a novel postbiotic	68:85	lactis BPL1: a novel postbiotic that reduces fat deposition via IGF-1 pathway.	55:132	lactis BPL1: a novel postbiotic that reduces fat deposition via IGF-1 pathway.
33620143	2	44	theme	risk	234:237	arg1	disorders					168:176	its related metabolic disorders	146:176	its related metabolic disorders	146:176	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	2	44	theme	risk	234:237	arg1	Obesity					134:140	Obesity	134:140	Obesity	134:140	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	2	44	theme	risk	234:237	arg1	factors					239:245	major risk factors	228:245	major risk factors for morbidity and mortality in the world population	228:297	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	2	44	theme	risk	234:237	arg1	diabetes					187:194	diabetes	187:194	diabetes	187:194	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	2	44	theme	risk	234:237	arg1	disease					215:221	cardiovascular disease	200:221	cardiovascular disease	200:221	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	2	45	from	mortality	265:273	arg1	population					288:297	the world population	278:297	the world population	278:297	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	2	46	theme	major	228:232	arg1	disorders					168:176	its related metabolic disorders	146:176	its related metabolic disorders	146:176	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	2	46	theme	major	228:232	arg1	Obesity					134:140	Obesity	134:140	Obesity	134:140	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	2	46	theme	major	228:232	arg1	factors					239:245	major risk factors	228:245	major risk factors for morbidity and mortality in the world population	228:297	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	2	46	theme	major	228:232	arg1	diabetes					187:194	diabetes	187:194	diabetes	187:194	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	2	46	theme	major	228:232	arg1	disease					215:221	cardiovascular disease	200:221	cardiovascular disease	200:221	Obesity and its related metabolic disorders, such as diabetes and cardiovascular disease, are major risk factors for morbidity and mortality in the world population.
33620143	6	47	from	changes	836:842	arg1	composition					860:870	LTA chemical composition	847:870	LTA chemical composition	847:870	This fat-reducing capacity of both BPL1 and LTA-BPL1 is abolished under glucose restriction, as a result of changes in LTA chemical composition.
34038893	5	0	theme	apatite	771:777	arg1	growth					779:784	apatite growth	771:784	apatite growth	771:784	The porous BG60 scaffolds system showed excellent bioactivity in anin vitrosimulated body fluid test in which the ions dissolved from the composite materials influenced apatite growth, countered the acidic pH, and increased material degradation.
34038893	6	1	theme	porous	910:915	arg1	scaffold					917:924	the porous scaffold	906:924	the porous scaffold	906:924	In anin vitrostudy with pre-osteoblasts cells (MC3T3-E1), the porous scaffold supported cell adhesion and proliferation.
34038893	5	2	theme	excellent	642:650	arg1	bioactivity					652:662	excellent bioactivity	642:662	excellent bioactivity in anin vitrosimulated body fluid test in which the ions dissolved from the composite materials influenced apatite growth, countered the acidic pH, and increased material degradation	642:845	The porous BG60 scaffolds system showed excellent bioactivity in anin vitrosimulated body fluid test in which the ions dissolved from the composite materials influenced apatite growth, countered the acidic pH, and increased material degradation.
34038893	11	3	theme	fast	1619:1622	arg1	applications					1649:1660	fast, early bone regeneration applications	1619:1660	fast, early bone regeneration applications	1619:1660	Our findings suggest that the porous BG scaffold could potentially be used as an injectable bone substitute for fast, early bone regeneration applications.
34038893	5	4	theme	composite	740:748	arg1	materials					750:758	the composite materials	736:758	the composite materials	736:758	The porous BG60 scaffolds system showed excellent bioactivity in anin vitrosimulated body fluid test in which the ions dissolved from the composite materials influenced apatite growth, countered the acidic pH, and increased material degradation.
34038893	0	5	theme	bioglass-derived	79:94	arg1	granules					96:103	porous 45s5 bioglass-derived granules	67:103	porous 45s5 bioglass-derived granules	67:103	Early-stage bone regeneration of hyaluronic acid supplemented with porous 45s5 bioglass-derived granules: an injectable system.
34038893	11	6	theme	bone	1631:1634	arg1	applications					1649:1660	fast, early bone regeneration applications	1619:1660	fast, early bone regeneration applications	1619:1660	Our findings suggest that the porous BG scaffold could potentially be used as an injectable bone substitute for fast, early bone regeneration applications.
34038893	10	7	theme	material	1449:1456	arg1	resorption					1421:1430	the resorption	1417:1430	the resorption of the composite material	1417:1456	The faster dissolution of the porous BG scaffold increased the resorption of the composite material and hence, facilitated bone tissue regeneration.
34038893	8	8	theme	bone	1201:1204	arg1	clues					1221:1225	clues	1221:1225	clues to tissue repair, and regeneration	1221:1260	Initialin vivodegradation of Hya promotes releasing ions which regulates the bone forming cells, clues to tissue repair, and regeneration.
34038893	8	8	theme	bone	1201:1204	arg1	cells					1214:1218	the bone forming cells	1197:1218	the bone forming cells	1197:1218	Initialin vivodegradation of Hya promotes releasing ions which regulates the bone forming cells, clues to tissue repair, and regeneration.
34038893	1	9	theme	injectable	153:162	arg1	system					180:185	an injectable bone substitute system	150:185	an injectable bone substitute system	150:185	In the present study, an injectable bone substitute system which utilized porous bioglass (BG)-derived granules supplemented with hyaluronic acid (Hya), was evaluated.
34038893	11	10	theme	regeneration	1636:1647	arg1	applications					1649:1660	fast, early bone regeneration applications	1619:1660	fast, early bone regeneration applications	1619:1660	Our findings suggest that the porous BG scaffold could potentially be used as an injectable bone substitute for fast, early bone regeneration applications.
34038893	10	11	theme	tissue	1486:1491	arg1	regeneration					1493:1504	bone tissue regeneration	1481:1504	bone tissue regeneration	1481:1504	The faster dissolution of the porous BG scaffold increased the resorption of the composite material and hence, facilitated bone tissue regeneration.
34038893	7	12	theme	post-implantation	971:987	arg1	study					989:993	A post-implantation study	969:993	A post-implantation study conducted in femoral defects	969:1022	A post-implantation study conducted in femoral defects showed implant degradation and surprisingly fast bone formation just after 2 weeks of implantation.
34038893	0	13	dep	system	120:125	arg1	regeneration					17:28	Early-stage bone regeneration	0:28	Early-stage bone regeneration of hyaluronic acid supplemented with porous 45s5 bioglass-derived granules	0:103	Early-stage bone regeneration of hyaluronic acid supplemented with porous 45s5 bioglass-derived granules: an injectable system.
34038893	1	14	theme	hyaluronic	258:267	arg1	Hya					275:277	Hya	275:277	Hya	275:277	In the present study, an injectable bone substitute system which utilized porous bioglass (BG)-derived granules supplemented with hyaluronic acid (Hya), was evaluated.
34038893	1	14	theme	hyaluronic	258:267	arg1	acid					269:272	hyaluronic acid	258:272	hyaluronic acid (Hya)	258:278	In the present study, an injectable bone substitute system which utilized porous bioglass (BG)-derived granules supplemented with hyaluronic acid (Hya), was evaluated.
34038893	9	15	theme	BG	1315:1316	arg1	granule					1318:1324	BG granule	1315:1324	BG granule	1315:1324	On the other hand it also prevent the scattering of BG granule after grafting at implant site.
34038893	5	16	theme	anin	667:670	arg1	test					698:701	anin vitrosimulated body fluid test	667:701	anin vitrosimulated body fluid test in which the ions dissolved from the composite materials influenced apatite growth, countered the acidic pH, and increased material degradation	667:845	The porous BG60 scaffolds system showed excellent bioactivity in anin vitrosimulated body fluid test in which the ions dissolved from the composite materials influenced apatite growth, countered the acidic pH, and increased material degradation.
34038893	10	17	theme	porous	1388:1393	arg1	scaffold					1398:1405	the porous BG scaffold	1384:1405	the porous BG scaffold	1384:1405	The faster dissolution of the porous BG scaffold increased the resorption of the composite material and hence, facilitated bone tissue regeneration.
34038893	3	18	theme	foreign	449:455	arg1	incorporation					463:475	any foreign phase incorporation	445:475	any foreign phase incorporation	445:475	The BG were synthesized by a simple and low sintering temperature process without any foreign phase incorporation.
34038893	8	19	theme	forming	1206:1212	arg1	clues					1221:1225	clues	1221:1225	clues to tissue repair, and regeneration	1221:1260	Initialin vivodegradation of Hya promotes releasing ions which regulates the bone forming cells, clues to tissue repair, and regeneration.
34038893	8	19	theme	forming	1206:1212	arg1	cells					1214:1218	the bone forming cells	1197:1218	the bone forming cells	1197:1218	Initialin vivodegradation of Hya promotes releasing ions which regulates the bone forming cells, clues to tissue repair, and regeneration.
34038893	9	20	theme	granule	1318:1324	arg1	scattering					1301:1310	the scattering	1297:1310	the scattering of BG granule	1297:1324	On the other hand it also prevent the scattering of BG granule after grafting at implant site.
34038893	5	21	theme	BG60	613:616	arg1	system					628:633	The porous BG60 scaffolds system	602:633	The porous BG60 scaffolds system	602:633	The porous BG60 scaffolds system showed excellent bioactivity in anin vitrosimulated body fluid test in which the ions dissolved from the composite materials influenced apatite growth, countered the acidic pH, and increased material degradation.
34038893	4	22	theme	thein	568:572	arg1	vivoperformance					585:599	thein vitroandin vivoperformance	568:599	thein vitroandin vivoperformance	568:599	Furthermore, the physical properties in the porous scaffold were optimized to investigate thein vitroandin vivoperformance.
34038893	1	23	theme	bone	164:167	arg1	system					180:185	an injectable bone substitute system	150:185	an injectable bone substitute system	150:185	In the present study, an injectable bone substitute system which utilized porous bioglass (BG)-derived granules supplemented with hyaluronic acid (Hya), was evaluated.
34038893	0	24	theme	injectable	109:118	arg1	system					120:125	an injectable system	106:125	an injectable system	106:125	Early-stage bone regeneration of hyaluronic acid supplemented with porous 45s5 bioglass-derived granules: an injectable system.
34038893	5	25	theme	scaffolds	618:626	arg1	system					628:633	The porous BG60 scaffolds system	602:633	The porous BG60 scaffolds system	602:633	The porous BG60 scaffolds system showed excellent bioactivity in anin vitrosimulated body fluid test in which the ions dissolved from the composite materials influenced apatite growth, countered the acidic pH, and increased material degradation.
34038893	1	26	link	-derived	222:229	arg1	granules					231:238	porous bioglass (BG)-derived granules	202:238	porous bioglass (BG)-derived granules supplemented with hyaluronic acid (Hya)	202:278	In the present study, an injectable bone substitute system which utilized porous bioglass (BG)-derived granules supplemented with hyaluronic acid (Hya), was evaluated.
34038893	11	27	dep	bone	1599:1602	arg1	substitute					1604:1613	substitute	1604:1613	substitute for fast, early bone regeneration applications	1604:1660	Our findings suggest that the porous BG scaffold could potentially be used as an injectable bone substitute for fast, early bone regeneration applications.
34038893	3	28	theme	low	403:405	arg1	process					429:435	a simple and low sintering temperature process	390:435	a simple and low sintering temperature process without any foreign phase incorporation	390:475	The BG were synthesized by a simple and low sintering temperature process without any foreign phase incorporation.
34038893	0	29	theme	bone	12:15	arg1	regeneration					17:28	Early-stage bone regeneration	0:28	Early-stage bone regeneration of hyaluronic acid supplemented with porous 45s5 bioglass-derived granules	0:103	Early-stage bone regeneration of hyaluronic acid supplemented with porous 45s5 bioglass-derived granules: an injectable system.
34038893	8	30	theme	releasing	1166:1174	arg1	ions					1176:1179	releasing ions	1166:1179	releasing ions which regulates the bone forming cells, clues to tissue repair, and regeneration	1166:1260	Initialin vivodegradation of Hya promotes releasing ions which regulates the bone forming cells, clues to tissue repair, and regeneration.
34038893	7	31	theme	bone	1073:1076	arg1	formation					1078:1086	surprisingly fast bone formation	1055:1086	surprisingly fast bone formation	1055:1086	A post-implantation study conducted in femoral defects showed implant degradation and surprisingly fast bone formation just after 2 weeks of implantation.
34038893	0	32	theme	Early-stage	0:10	arg1	regeneration					17:28	Early-stage bone regeneration	0:28	Early-stage bone regeneration of hyaluronic acid supplemented with porous 45s5 bioglass-derived granules	0:103	Early-stage bone regeneration of hyaluronic acid supplemented with porous 45s5 bioglass-derived granules: an injectable system.
34038893	8	33	theme	Initialin	1124:1132	arg1	vivodegradation					1134:1148	Initialin vivodegradation	1124:1148	Initialin vivodegradation of Hya	1124:1155	Initialin vivodegradation of Hya promotes releasing ions which regulates the bone forming cells, clues to tissue repair, and regeneration.
34038893	7	34	theme	implantation	1110:1121	arg1	weeks					1101:1105	2 weeks	1099:1105	2 weeks of implantation	1099:1121	A post-implantation study conducted in femoral defects showed implant degradation and surprisingly fast bone formation just after 2 weeks of implantation.
34038893	2	35	theme	cell	348:351	arg1	motility					353:360	cell motility	348:360	cell motility	348:360	Hya plays ultimate role in wound healing, promoting cell motility.
34038893	4	36	theme	porous	522:527	arg1	scaffold					529:536	the porous scaffold	518:536	the porous scaffold	518:536	Furthermore, the physical properties in the porous scaffold were optimized to investigate thein vitroandin vivoperformance.
34038893	5	37	theme	porous	606:611	arg1	system					628:633	The porous BG60 scaffolds system	602:633	The porous BG60 scaffolds system	602:633	The porous BG60 scaffolds system showed excellent bioactivity in anin vitrosimulated body fluid test in which the ions dissolved from the composite materials influenced apatite growth, countered the acidic pH, and increased material degradation.
34038893	10	38	theme	scaffold	1398:1405	arg1	dissolution					1369:1379	The faster dissolution	1358:1379	The faster dissolution of the porous BG scaffold	1358:1405	The faster dissolution of the porous BG scaffold increased the resorption of the composite material and hence, facilitated bone tissue regeneration.
34038893	5	39	theme	material	826:833	arg1	degradation					835:845	material degradation	826:845	material degradation	826:845	The porous BG60 scaffolds system showed excellent bioactivity in anin vitrosimulated body fluid test in which the ions dissolved from the composite materials influenced apatite growth, countered the acidic pH, and increased material degradation.
34038893	6	40	theme	pre-osteoblasts	872:886	arg1	MC3T3-E1					895:902	MC3T3-E1	895:902	MC3T3-E1	895:902	In anin vitrostudy with pre-osteoblasts cells (MC3T3-E1), the porous scaffold supported cell adhesion and proliferation.
34038893	6	40	theme	pre-osteoblasts	872:886	arg1	cells					888:892	pre-osteoblasts cells	872:892	pre-osteoblasts cells (MC3T3-E1)	872:903	In anin vitrostudy with pre-osteoblasts cells (MC3T3-E1), the porous scaffold supported cell adhesion and proliferation.
34038893	2	41	theme	wound	323:327	arg1	healing					329:335	wound healing	323:335	wound healing	323:335	Hya plays ultimate role in wound healing, promoting cell motility.
34038893	0	42	theme	acid	44:47	arg1	regeneration					17:28	Early-stage bone regeneration	0:28	Early-stage bone regeneration of hyaluronic acid supplemented with porous 45s5 bioglass-derived granules	0:103	Early-stage bone regeneration of hyaluronic acid supplemented with porous 45s5 bioglass-derived granules: an injectable system.
34038893	1	43	dep	system	180:185	arg1	substitute					169:178	substitute	169:178	substitute	169:178	In the present study, an injectable bone substitute system which utilized porous bioglass (BG)-derived granules supplemented with hyaluronic acid (Hya), was evaluated.
34038893	3	44	theme	simple	392:397	arg1	process					429:435	a simple and low sintering temperature process	390:435	a simple and low sintering temperature process without any foreign phase incorporation	390:475	The BG were synthesized by a simple and low sintering temperature process without any foreign phase incorporation.
34038893	3	45	theme	sintering	407:415	arg1	process					429:435	a simple and low sintering temperature process	390:435	a simple and low sintering temperature process without any foreign phase incorporation	390:475	The BG were synthesized by a simple and low sintering temperature process without any foreign phase incorporation.
34038893	0	46	theme	hyaluronic	33:42	arg1	acid					44:47	hyaluronic acid	33:47	hyaluronic acid	33:47	Early-stage bone regeneration of hyaluronic acid supplemented with porous 45s5 bioglass-derived granules: an injectable system.
34038893	8	47	theme	tissue	1230:1235	arg1	repair					1237:1242	tissue repair	1230:1242	tissue repair	1230:1242	Initialin vivodegradation of Hya promotes releasing ions which regulates the bone forming cells, clues to tissue repair, and regeneration.
34038893	9	48	theme	implant	1344:1350	arg1	site					1352:1355	implant site	1344:1355	implant site	1344:1355	On the other hand it also prevent the scattering of BG granule after grafting at implant site.
34038893	7	49	theme	implant	1031:1037	arg1	degradation					1039:1049	implant degradation	1031:1049	implant degradation	1031:1049	A post-implantation study conducted in femoral defects showed implant degradation and surprisingly fast bone formation just after 2 weeks of implantation.
34038893	3	50	theme	temperature	417:427	arg1	process					429:435	a simple and low sintering temperature process	390:435	a simple and low sintering temperature process without any foreign phase incorporation	390:475	The BG were synthesized by a simple and low sintering temperature process without any foreign phase incorporation.
34038893	4	51	from	properties	504:513	arg1	scaffold					529:536	the porous scaffold	518:536	the porous scaffold	518:536	Furthermore, the physical properties in the porous scaffold were optimized to investigate thein vitroandin vivoperformance.
34038893	4	52	theme	physical	495:502	arg1	properties					504:513	the physical properties	491:513	the physical properties in the porous scaffold	491:536	Furthermore, the physical properties in the porous scaffold were optimized to investigate thein vitroandin vivoperformance.
34038893	5	53	theme	vitrosimulated	672:685	arg1	test					698:701	anin vitrosimulated body fluid test	667:701	anin vitrosimulated body fluid test in which the ions dissolved from the composite materials influenced apatite growth, countered the acidic pH, and increased material degradation	667:845	The porous BG60 scaffolds system showed excellent bioactivity in anin vitrosimulated body fluid test in which the ions dissolved from the composite materials influenced apatite growth, countered the acidic pH, and increased material degradation.
34038893	6	54	theme	anin	851:854	arg1	vitrostudy					856:865	anin vitrostudy	851:865	anin vitrostudy with pre-osteoblasts cells (MC3T3-E1)	851:903	In anin vitrostudy with pre-osteoblasts cells (MC3T3-E1), the porous scaffold supported cell adhesion and proliferation.
34038893	0	55	link	bioglass-derived	79:94	arg1	granules					96:103	porous 45s5 bioglass-derived granules	67:103	porous 45s5 bioglass-derived granules	67:103	Early-stage bone regeneration of hyaluronic acid supplemented with porous 45s5 bioglass-derived granules: an injectable system.
34038893	3	56	theme	phase	457:461	arg1	incorporation					463:475	any foreign phase incorporation	445:475	any foreign phase incorporation	445:475	The BG were synthesized by a simple and low sintering temperature process without any foreign phase incorporation.
34038893	5	57	theme	acidic	801:806	arg1	pH					808:809	the acidic pH	797:809	the acidic pH	797:809	The porous BG60 scaffolds system showed excellent bioactivity in anin vitrosimulated body fluid test in which the ions dissolved from the composite materials influenced apatite growth, countered the acidic pH, and increased material degradation.
34038893	6	58	theme	cell	936:939	arg1	adhesion					941:948	cell adhesion	936:948	cell adhesion	936:948	In anin vitrostudy with pre-osteoblasts cells (MC3T3-E1), the porous scaffold supported cell adhesion and proliferation.
34038893	5	59	theme	body	687:690	arg1	test					698:701	anin vitrosimulated body fluid test	667:701	anin vitrosimulated body fluid test in which the ions dissolved from the composite materials influenced apatite growth, countered the acidic pH, and increased material degradation	667:845	The porous BG60 scaffolds system showed excellent bioactivity in anin vitrosimulated body fluid test in which the ions dissolved from the composite materials influenced apatite growth, countered the acidic pH, and increased material degradation.
34038893	4	60	theme	vitroandin	574:583	arg1	vivoperformance					585:599	thein vitroandin vivoperformance	568:599	thein vitroandin vivoperformance	568:599	Furthermore, the physical properties in the porous scaffold were optimized to investigate thein vitroandin vivoperformance.
34038893	1	61	used	utilized	193:200	arg2	system					180:185	an injectable bone substitute system	150:185	an injectable bone substitute system	150:185	In the present study, an injectable bone substitute system which utilized porous bioglass (BG)-derived granules supplemented with hyaluronic acid (Hya), was evaluated.
34038893	11	62	theme	BG	1544:1545	arg1	scaffold					1547:1554	the porous BG scaffold	1533:1554	the porous BG scaffold	1533:1554	Our findings suggest that the porous BG scaffold could potentially be used as an injectable bone substitute for fast, early bone regeneration applications.
34038893	11	62	theme	BG	1544:1545	arg1	bone					1599:1602	an injectable bone	1585:1602	an injectable bone substitute for fast, early bone regeneration applications	1585:1660	Our findings suggest that the porous BG scaffold could potentially be used as an injectable bone substitute for fast, early bone regeneration applications.
34038893	10	63	theme	composite	1439:1447	arg1	material					1449:1456	the composite material	1435:1456	the composite material	1435:1456	The faster dissolution of the porous BG scaffold increased the resorption of the composite material and hence, facilitated bone tissue regeneration.
34038893	5	64	from	bioactivity	652:662	arg1	test					698:701	anin vitrosimulated body fluid test	667:701	anin vitrosimulated body fluid test in which the ions dissolved from the composite materials influenced apatite growth, countered the acidic pH, and increased material degradation	667:845	The porous BG60 scaffolds system showed excellent bioactivity in anin vitrosimulated body fluid test in which the ions dissolved from the composite materials influenced apatite growth, countered the acidic pH, and increased material degradation.
34038893	5	65	theme	fluid	692:696	arg1	test					698:701	anin vitrosimulated body fluid test	667:701	anin vitrosimulated body fluid test in which the ions dissolved from the composite materials influenced apatite growth, countered the acidic pH, and increased material degradation	667:845	The porous BG60 scaffolds system showed excellent bioactivity in anin vitrosimulated body fluid test in which the ions dissolved from the composite materials influenced apatite growth, countered the acidic pH, and increased material degradation.
34038893	11	66	used	used	1577:1580	arg2	bone					1599:1602	an injectable bone	1585:1602	an injectable bone substitute for fast, early bone regeneration applications	1585:1660	Our findings suggest that the porous BG scaffold could potentially be used as an injectable bone substitute for fast, early bone regeneration applications.
34038893	11	66	used	used	1577:1580	arg2	scaffold					1547:1554	the porous BG scaffold	1533:1554	the porous BG scaffold	1533:1554	Our findings suggest that the porous BG scaffold could potentially be used as an injectable bone substitute for fast, early bone regeneration applications.
34038893	11	67	theme	porous	1537:1542	arg1	scaffold					1547:1554	the porous BG scaffold	1533:1554	the porous BG scaffold	1533:1554	Our findings suggest that the porous BG scaffold could potentially be used as an injectable bone substitute for fast, early bone regeneration applications.
34038893	11	67	theme	porous	1537:1542	arg1	bone					1599:1602	an injectable bone	1585:1602	an injectable bone substitute for fast, early bone regeneration applications	1585:1660	Our findings suggest that the porous BG scaffold could potentially be used as an injectable bone substitute for fast, early bone regeneration applications.
34038893	10	68	theme	BG	1395:1396	arg1	scaffold					1398:1405	the porous BG scaffold	1384:1405	the porous BG scaffold	1384:1405	The faster dissolution of the porous BG scaffold increased the resorption of the composite material and hence, facilitated bone tissue regeneration.
34038893	2	69	theme	ultimate	306:313	arg1	role					315:318	ultimate role	306:318	ultimate role	306:318	Hya plays ultimate role in wound healing, promoting cell motility.
34038893	10	70	dep	increased	1407:1415	arg1	facilitated					1469:1479	facilitated	1469:1479	facilitated bone tissue regeneration	1469:1504	The faster dissolution of the porous BG scaffold increased the resorption of the composite material and hence, facilitated bone tissue regeneration.
34038893	11	71	theme	injectable	1588:1597	arg1	bone					1599:1602	an injectable bone	1585:1602	an injectable bone substitute for fast, early bone regeneration applications	1585:1660	Our findings suggest that the porous BG scaffold could potentially be used as an injectable bone substitute for fast, early bone regeneration applications.
34038893	11	71	theme	injectable	1588:1597	arg1	scaffold					1547:1554	the porous BG scaffold	1533:1554	the porous BG scaffold	1533:1554	Our findings suggest that the porous BG scaffold could potentially be used as an injectable bone substitute for fast, early bone regeneration applications.
34038893	8	72	theme	Hya	1153:1155	arg1	vivodegradation					1134:1148	Initialin vivodegradation	1124:1148	Initialin vivodegradation of Hya	1124:1155	Initialin vivodegradation of Hya promotes releasing ions which regulates the bone forming cells, clues to tissue repair, and regeneration.
34038893	1	73	theme	present	135:141	arg1	study					143:147	the present study	131:147	the present study	131:147	In the present study, an injectable bone substitute system which utilized porous bioglass (BG)-derived granules supplemented with hyaluronic acid (Hya), was evaluated.
34038893	0	74	theme	45s5	74:77	arg1	granules					96:103	porous 45s5 bioglass-derived granules	67:103	porous 45s5 bioglass-derived granules	67:103	Early-stage bone regeneration of hyaluronic acid supplemented with porous 45s5 bioglass-derived granules: an injectable system.
34038893	7	75	theme	fast	1068:1071	arg1	formation					1078:1086	surprisingly fast bone formation	1055:1086	surprisingly fast bone formation	1055:1086	A post-implantation study conducted in femoral defects showed implant degradation and surprisingly fast bone formation just after 2 weeks of implantation.
34038893	7	76	theme	femoral	1008:1014	arg1	defects					1016:1022	femoral defects	1008:1022	femoral defects	1008:1022	A post-implantation study conducted in femoral defects showed implant degradation and surprisingly fast bone formation just after 2 weeks of implantation.
34038893	9	77	theme	other	1270:1274	arg1	hand					1276:1279	the other hand	1266:1279	the other hand	1266:1279	On the other hand it also prevent the scattering of BG granule after grafting at implant site.
34038893	1	78	theme	-derived	222:229	arg1	granules					231:238	porous bioglass (BG)-derived granules	202:238	porous bioglass (BG)-derived granules supplemented with hyaluronic acid (Hya)	202:278	In the present study, an injectable bone substitute system which utilized porous bioglass (BG)-derived granules supplemented with hyaluronic acid (Hya), was evaluated.
34038893	0	79	theme	porous	67:72	arg1	granules					96:103	porous 45s5 bioglass-derived granules	67:103	porous 45s5 bioglass-derived granules	67:103	Early-stage bone regeneration of hyaluronic acid supplemented with porous 45s5 bioglass-derived granules: an injectable system.
34038893	10	80	theme	bone	1481:1484	arg1	regeneration					1493:1504	bone tissue regeneration	1481:1504	bone tissue regeneration	1481:1504	The faster dissolution of the porous BG scaffold increased the resorption of the composite material and hence, facilitated bone tissue regeneration.
34038893	10	81	theme	faster	1362:1367	arg1	dissolution					1369:1379	The faster dissolution	1358:1379	The faster dissolution of the porous BG scaffold	1358:1405	The faster dissolution of the porous BG scaffold increased the resorption of the composite material and hence, facilitated bone tissue regeneration.
34038893	11	82	dep	fast	1619:1622	arg1	early					1625:1629	early	1625:1629	early	1625:1629	Our findings suggest that the porous BG scaffold could potentially be used as an injectable bone substitute for fast, early bone regeneration applications.
34038893	6	83	with	vitrostudy	856:865	arg1	MC3T3-E1					895:902	MC3T3-E1	895:902	MC3T3-E1	895:902	In anin vitrostudy with pre-osteoblasts cells (MC3T3-E1), the porous scaffold supported cell adhesion and proliferation.
34038893	6	83	with	vitrostudy	856:865	arg1	cells					888:892	pre-osteoblasts cells	872:892	pre-osteoblasts cells (MC3T3-E1)	872:903	In anin vitrostudy with pre-osteoblasts cells (MC3T3-E1), the porous scaffold supported cell adhesion and proliferation.
34732247	2	0	with	combination	409:419	arg1	phosphate					460:468	calcium phosphate	452:468	calcium phosphate	452:468	The combination of dietary FOS and inulin with calcium phosphate was reported to stimulate commensal Lactobacillus populations and protect the host against pathogenic Enterobacteriaceae, but little is known about the effects of GOS in diets with a different level of calcium phosphate.
34732247	7	1	from	increases	1681:1689	arg1	levels					1718:1723	lactic and butyric acid levels	1694:1723	lactic and butyric acid levels	1694:1723	In contrast, relatively low dietary calcium phosphate levels promoted the abundance of mucin degrading genera like Akkermansia and Bacteroides, and resulted in increased fecal propionic acid levels and modest increases in lactic and butyric acid levels.
34732247	9	2	theme	gut	2151:2153	arg1	microbiota					2155:2164	the gut microbiota	2147:2164	the gut microbiota	2147:2164	CONCLUSIONS Despite the prebiotic's substantial difference in chemical structure, sugar composition, oligomer size, and the microbial degradation pathway involved in their utilization, inulin and GOS modulated the gut microbiota very similarly, in a manner that strongly depended on the dietary calcium phosphate level.
34732247	6	3	theme	RESULTS	1245:1251	arg1	rats					1261:1264	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets	1245:1308	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets	1245:1308	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets revealed that diets rich in calcium phosphate favored members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels.
34732247	5	4	theme	acid	1194:1197	arg1	profiles					1199:1206	the organic acid profiles	1182:1206	the organic acid profiles of the fecal material	1182:1228	In tandem, the organic acid profiles of the fecal material were analyzed.
34732247	6	5	dep	non-supplemented	1266:1281	arg1	prebiotic-added					1287:1301	prebiotic-added	1287:1301	prebiotic-added	1287:1301	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets revealed that diets rich in calcium phosphate favored members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels.
34732247	10	6	theme	detailed	2309:2316	arg1	information					2323:2333	detailed diet information	2309:2333	detailed diet information	2309:2333	Therefore, our study implies that the collection of detailed diet information including micronutrient balance is necessary to correctly assess diet-driven microbiota analysis.
34732247	8	7	theme	phosphate	1868:1876	arg1	diets					1878:1882	only high calcium phosphate diets	1850:1882	only high calcium phosphate diets	1850:1882	Irrespective of the calcium phosphate levels, supplementation with GOS or inulin strongly stimulated Bifidobacterium, while only high calcium phosphate diets increased the endogenous Faecalibaculum populations.
34732247	0	8	theme	Dietary	0:6	arg1	phosphate					16:24	Dietary calcium phosphate	0:24	Dietary calcium phosphate	0:24	Dietary calcium phosphate strongly impacts gut microbiome changes elicited by inulin and galacto-oligosaccharides consumption.
34732247	2	9	theme	different	653:661	arg1	level					663:667	a different level	651:667	a different level of calcium phosphate	651:688	The combination of dietary FOS and inulin with calcium phosphate was reported to stimulate commensal Lactobacillus populations and protect the host against pathogenic Enterobacteriaceae, but little is known about the effects of GOS in diets with a different level of calcium phosphate.
34732247	9	10	theme	sugar	2019:2023	arg1	composition					2025:2035	sugar composition	2019:2035	sugar composition	2019:2035	CONCLUSIONS Despite the prebiotic's substantial difference in chemical structure, sugar composition, oligomer size, and the microbial degradation pathway involved in their utilization, inulin and GOS modulated the gut microbiota very similarly, in a manner that strongly depended on the dietary calcium phosphate level.
34732247	4	11	theme	experimental	938:949	arg1	diets					951:955	the respective experimental diets	923:955	the respective experimental diets	923:955	Rats were acclimatized to the respective experimental diets for 14 days, after which fecal material was collected, DNA was extracted from fecal material, and the V3‑V4 region of the bacterial 16S rRNA gene was amplified with PCR, followed by microbial composition analysis.
34732247	4	12	theme	16S	1089:1091	arg1	gene					1098:1101	the bacterial 16S rRNA gene	1075:1101	the bacterial 16S rRNA gene	1075:1101	Rats were acclimatized to the respective experimental diets for 14 days, after which fecal material was collected, DNA was extracted from fecal material, and the V3‑V4 region of the bacterial 16S rRNA gene was amplified with PCR, followed by microbial composition analysis.
34732247	8	13	theme	endogenous	1898:1907	arg1	populations					1924:1934	the endogenous Faecalibaculum populations	1894:1934	the endogenous Faecalibaculum populations	1894:1934	Irrespective of the calcium phosphate levels, supplementation with GOS or inulin strongly stimulated Bifidobacterium, while only high calcium phosphate diets increased the endogenous Faecalibaculum populations.
34732247	10	14	theme	information	2323:2333	arg1	collection					2295:2304	the collection	2291:2304	the collection of detailed diet information including micronutrient balance	2291:2365	Therefore, our study implies that the collection of detailed diet information including micronutrient balance is necessary to correctly assess diet-driven microbiota analysis.
34732247	10	14	theme	information	2323:2333	arg1	necessary					2370:2378	necessary	2370:2378	necessary	2370:2378	Therefore, our study implies that the collection of detailed diet information including micronutrient balance is necessary to correctly assess diet-driven microbiota analysis.
34732247	4	15	theme	gene	1098:1101	arg1	region					1065:1070	the V3‑V4 region	1055:1070	the V3‑V4 region of the bacterial 16S rRNA gene	1055:1101	Rats were acclimatized to the respective experimental diets for 14 days, after which fecal material was collected, DNA was extracted from fecal material, and the V3‑V4 region of the bacterial 16S rRNA gene was amplified with PCR, followed by microbial composition analysis.
34732247	3	16	dep	low	833:835	arg1	mmol/kg					841:847	30 mmol/kg	838:847	30 mmol/kg	838:847	METHODS We investigated the microbiome changes elicited by dietary supplementation with GOS or inulin using diets with high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats.
34732247	9	17	theme	substantial	1973:1983	arg1	difference					1985:1994	the prebiotic's substantial difference	1957:1994	the prebiotic's substantial difference in chemical structure, sugar composition, oligomer size, and the microbial degradation pathway involved in their utilization, inulin and GOS	1957:2135	CONCLUSIONS Despite the prebiotic's substantial difference in chemical structure, sugar composition, oligomer size, and the microbial degradation pathway involved in their utilization, inulin and GOS modulated the gut microbiota very similarly, in a manner that strongly depended on the dietary calcium phosphate level.
34732247	3	18	theme	high	810:813	arg1	levels					868:873	high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels	810:873	high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats	810:894	METHODS We investigated the microbiome changes elicited by dietary supplementation with GOS or inulin using diets with high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats.
34732247	8	19	theme	calcium	1746:1752	arg1	levels					1764:1769	the calcium phosphate levels	1742:1769	the calcium phosphate levels	1742:1769	Irrespective of the calcium phosphate levels, supplementation with GOS or inulin strongly stimulated Bifidobacterium, while only high calcium phosphate diets increased the endogenous Faecalibaculum populations.
34732247	8	20	theme	calcium	1860:1866	arg1	diets					1878:1882	only high calcium phosphate diets	1850:1882	only high calcium phosphate diets	1850:1882	Irrespective of the calcium phosphate levels, supplementation with GOS or inulin strongly stimulated Bifidobacterium, while only high calcium phosphate diets increased the endogenous Faecalibaculum populations.
34732247	3	21	theme	low	833:835	arg1	levels					868:873	high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels	810:873	high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats	810:894	METHODS We investigated the microbiome changes elicited by dietary supplementation with GOS or inulin using diets with high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats.
34732247	3	22	theme	phosphate	858:866	arg1	levels					868:873	high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels	810:873	high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats	810:894	METHODS We investigated the microbiome changes elicited by dietary supplementation with GOS or inulin using diets with high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats.
34732247	7	23	theme	degrading	1565:1573	arg1	genera					1575:1580	mucin degrading genera	1559:1580	mucin degrading genera	1559:1580	In contrast, relatively low dietary calcium phosphate levels promoted the abundance of mucin degrading genera like Akkermansia and Bacteroides, and resulted in increased fecal propionic acid levels and modest increases in lactic and butyric acid levels.
34732247	4	24	theme	fecal	982:986	arg1	material					988:995	fecal material	982:995	fecal material	982:995	Rats were acclimatized to the respective experimental diets for 14 days, after which fecal material was collected, DNA was extracted from fecal material, and the V3‑V4 region of the bacterial 16S rRNA gene was amplified with PCR, followed by microbial composition analysis.
34732247	9	25	theme	calcium	2232:2238	arg1	level					2250:2254	the dietary calcium phosphate level	2220:2254	the dietary calcium phosphate level	2220:2254	CONCLUSIONS Despite the prebiotic's substantial difference in chemical structure, sugar composition, oligomer size, and the microbial degradation pathway involved in their utilization, inulin and GOS modulated the gut microbiota very similarly, in a manner that strongly depended on the dietary calcium phosphate level.
34732247	7	26	theme	lactic	1694:1699	arg1	levels					1718:1723	lactic and butyric acid levels	1694:1723	lactic and butyric acid levels	1694:1723	In contrast, relatively low dietary calcium phosphate levels promoted the abundance of mucin degrading genera like Akkermansia and Bacteroides, and resulted in increased fecal propionic acid levels and modest increases in lactic and butyric acid levels.
34732247	4	27	theme	microbial	1139:1147	arg1	analysis					1161:1168	microbial composition analysis	1139:1168	microbial composition analysis	1139:1168	Rats were acclimatized to the respective experimental diets for 14 days, after which fecal material was collected, DNA was extracted from fecal material, and the V3‑V4 region of the bacterial 16S rRNA gene was amplified with PCR, followed by microbial composition analysis.
34732247	8	28	theme	high	1855:1858	arg1	diets					1878:1882	only high calcium phosphate diets	1850:1882	only high calcium phosphate diets	1850:1882	Irrespective of the calcium phosphate levels, supplementation with GOS or inulin strongly stimulated Bifidobacterium, while only high calcium phosphate diets increased the endogenous Faecalibaculum populations.
34732247	3	29	theme	Wistar	884:889	arg1	rats					891:894	adult Wistar rats	878:894	adult Wistar rats	878:894	METHODS We investigated the microbiome changes elicited by dietary supplementation with GOS or inulin using diets with high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats.
34732247	11	30	dep	Abstract	2439:2446	arg1	Video					2433:2437	Video	2433:2437	Video	2433:2437	Video Abstract.
34732247	6	31	theme	calcium	1338:1344	arg1	phosphate					1346:1354	calcium phosphate	1338:1354	calcium phosphate	1338:1354	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets revealed that diets rich in calcium phosphate favored members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels.
34732247	3	32	dep	high	810:813	arg1	mmol/kg					820:826	100 mmol/kg	816:826	100 mmol/kg	816:826	METHODS We investigated the microbiome changes elicited by dietary supplementation with GOS or inulin using diets with high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats.
34732247	2	33	theme	pathogenic	561:570	arg1	Enterobacteriaceae					572:589	pathogenic Enterobacteriaceae	561:589	pathogenic Enterobacteriaceae	561:589	The combination of dietary FOS and inulin with calcium phosphate was reported to stimulate commensal Lactobacillus populations and protect the host against pathogenic Enterobacteriaceae, but little is known about the effects of GOS in diets with a different level of calcium phosphate.
34732247	2	34	theme	calcium	672:678	arg1	phosphate					680:688	calcium phosphate	672:688	calcium phosphate	672:688	The combination of dietary FOS and inulin with calcium phosphate was reported to stimulate commensal Lactobacillus populations and protect the host against pathogenic Enterobacteriaceae, but little is known about the effects of GOS in diets with a different level of calcium phosphate.
34732247	7	35	theme	butyric	1705:1711	arg1	levels					1718:1723	lactic and butyric acid levels	1694:1723	lactic and butyric acid levels	1694:1723	In contrast, relatively low dietary calcium phosphate levels promoted the abundance of mucin degrading genera like Akkermansia and Bacteroides, and resulted in increased fecal propionic acid levels and modest increases in lactic and butyric acid levels.
34732247	2	36	theme	FOS	432:434	arg1	combination					409:419	The combination	405:419	The combination of dietary FOS and inulin with calcium phosphate	405:468	The combination of dietary FOS and inulin with calcium phosphate was reported to stimulate commensal Lactobacillus populations and protect the host against pathogenic Enterobacteriaceae, but little is known about the effects of GOS in diets with a different level of calcium phosphate.
34732247	6	37	theme	rich	1330:1333	arg1	diets					1324:1328	diets	1324:1328	diets rich in calcium phosphate	1324:1354	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets revealed that diets rich in calcium phosphate favored members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels.
34732247	6	38	from	phosphate	1346:1354	arg1	rich					1330:1333	rich	1330:1333	rich	1330:1333	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets revealed that diets rich in calcium phosphate favored members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels.
34732247	0	39	theme	gut	43:45	arg1	changes					58:64	gut microbiome changes	43:64	gut microbiome changes elicited by inulin and galacto-oligosaccharides consumption	43:124	Dietary calcium phosphate strongly impacts gut microbiome changes elicited by inulin and galacto-oligosaccharides consumption.
34732247	7	40	theme	acid	1658:1661	arg1	levels					1663:1668	increased fecal propionic acid levels	1632:1668	increased fecal propionic acid levels	1632:1668	In contrast, relatively low dietary calcium phosphate levels promoted the abundance of mucin degrading genera like Akkermansia and Bacteroides, and resulted in increased fecal propionic acid levels and modest increases in lactic and butyric acid levels.
34732247	10	41	theme	microbiota	2412:2421	arg1	analysis					2423:2430	diet-driven microbiota analysis	2400:2430	diet-driven microbiota analysis	2400:2430	Therefore, our study implies that the collection of detailed diet information including micronutrient balance is necessary to correctly assess diet-driven microbiota analysis.
34732247	6	42	theme	succinic	1418:1425	arg1	acid					1459:1462	lactic, succinic, acetic, propionic, and butyric acid	1410:1462	increased fecal lactic, succinic, acetic, propionic, and butyric acid levels	1394:1469	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets revealed that diets rich in calcium phosphate favored members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels.
34732247	3	43	with	diets	799:803	arg1	levels					868:873	high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels	810:873	high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats	810:894	METHODS We investigated the microbiome changes elicited by dietary supplementation with GOS or inulin using diets with high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats.
34732247	0	44	dep	inulin	78:83	arg1	consumption					114:124	consumption	114:124	consumption	114:124	Dietary calcium phosphate strongly impacts gut microbiome changes elicited by inulin and galacto-oligosaccharides consumption.
34732247	7	45	theme	fecal	1642:1646	arg1	acid					1658:1661	fecal propionic acid	1642:1661	increased fecal propionic acid levels	1632:1668	In contrast, relatively low dietary calcium phosphate levels promoted the abundance of mucin degrading genera like Akkermansia and Bacteroides, and resulted in increased fecal propionic acid levels and modest increases in lactic and butyric acid levels.
34732247	9	46	theme	microbial	2061:2069	arg1	pathway					2083:2089	the microbial degradation pathway	2057:2089	the microbial degradation pathway involved in their utilization, inulin and GOS	2057:2135	CONCLUSIONS Despite the prebiotic's substantial difference in chemical structure, sugar composition, oligomer size, and the microbial degradation pathway involved in their utilization, inulin and GOS modulated the gut microbiota very similarly, in a manner that strongly depended on the dietary calcium phosphate level.
34732247	2	47	theme	calcium	452:458	arg1	phosphate					460:468	calcium phosphate	452:468	calcium phosphate	452:468	The combination of dietary FOS and inulin with calcium phosphate was reported to stimulate commensal Lactobacillus populations and protect the host against pathogenic Enterobacteriaceae, but little is known about the effects of GOS in diets with a different level of calcium phosphate.
34732247	6	48	theme	lactic	1410:1415	arg1	acid					1459:1462	lactic, succinic, acetic, propionic, and butyric acid	1410:1462	increased fecal lactic, succinic, acetic, propionic, and butyric acid levels	1394:1469	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets revealed that diets rich in calcium phosphate favored members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels.
34732247	1	49	theme	bifidobacteria	320:333	arg1	stimulation					305:315	stimulation	305:315	stimulation of bifidobacteria and lactobacilli	305:350	BACKGROUND Fructo-oligosaccharides (FOS), inulin, and galacto-oligosaccharides (GOS) are widely recognized prebiotics that profoundly affect the intestinal microbiota, including stimulation of bifidobacteria and lactobacilli, and are reported to elicit several health benefits.
34732247	7	50	theme	dietary	1500:1506	arg1	levels					1526:1531	relatively low dietary calcium phosphate levels	1485:1531	relatively low dietary calcium phosphate levels	1485:1531	In contrast, relatively low dietary calcium phosphate levels promoted the abundance of mucin degrading genera like Akkermansia and Bacteroides, and resulted in increased fecal propionic acid levels and modest increases in lactic and butyric acid levels.
34732247	1	51	theme	BACKGROUND	127:136	arg1	FOS					163:165	FOS	163:165	FOS	163:165	BACKGROUND Fructo-oligosaccharides (FOS), inulin, and galacto-oligosaccharides (GOS) are widely recognized prebiotics that profoundly affect the intestinal microbiota, including stimulation of bifidobacteria and lactobacilli, and are reported to elicit several health benefits.
34732247	1	51	theme	BACKGROUND	127:136	arg1	inulin					169:174	inulin	169:174	inulin	169:174	BACKGROUND Fructo-oligosaccharides (FOS), inulin, and galacto-oligosaccharides (GOS) are widely recognized prebiotics that profoundly affect the intestinal microbiota, including stimulation of bifidobacteria and lactobacilli, and are reported to elicit several health benefits.
34732247	1	51	theme	BACKGROUND	127:136	arg1	Fructo-oligosaccharides					138:160	BACKGROUND Fructo-oligosaccharides	127:160	BACKGROUND Fructo-oligosaccharides (FOS)	127:166	BACKGROUND Fructo-oligosaccharides (FOS), inulin, and galacto-oligosaccharides (GOS) are widely recognized prebiotics that profoundly affect the intestinal microbiota, including stimulation of bifidobacteria and lactobacilli, and are reported to elicit several health benefits.
34732247	1	51	theme	BACKGROUND	127:136	arg1	galacto-oligosaccharides					181:204	galacto-oligosaccharides	181:204	galacto-oligosaccharides (GOS)	181:210	BACKGROUND Fructo-oligosaccharides (FOS), inulin, and galacto-oligosaccharides (GOS) are widely recognized prebiotics that profoundly affect the intestinal microbiota, including stimulation of bifidobacteria and lactobacilli, and are reported to elicit several health benefits.
34732247	1	51	theme	BACKGROUND	127:136	arg1	prebiotics					234:243	widely recognized prebiotics	216:243	widely recognized prebiotics that profoundly affect the intestinal microbiota, including stimulation of bifidobacteria and lactobacilli, and are reported to elicit several health benefits	216:402	BACKGROUND Fructo-oligosaccharides (FOS), inulin, and galacto-oligosaccharides (GOS) are widely recognized prebiotics that profoundly affect the intestinal microbiota, including stimulation of bifidobacteria and lactobacilli, and are reported to elicit several health benefits.
34732247	2	52	theme	inulin	440:445	arg1	combination					409:419	The combination	405:419	The combination of dietary FOS and inulin with calcium phosphate	405:468	The combination of dietary FOS and inulin with calcium phosphate was reported to stimulate commensal Lactobacillus populations and protect the host against pathogenic Enterobacteriaceae, but little is known about the effects of GOS in diets with a different level of calcium phosphate.
34732247	6	53	theme	increased	1394:1402	arg1	levels					1464:1469	increased fecal lactic, succinic, acetic, propionic, and butyric acid levels	1394:1469	increased fecal lactic, succinic, acetic, propionic, and butyric acid levels	1394:1469	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets revealed that diets rich in calcium phosphate favored members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels.
34732247	1	54	theme	lactobacilli	339:350	arg1	stimulation					305:315	stimulation	305:315	stimulation of bifidobacteria and lactobacilli	305:350	BACKGROUND Fructo-oligosaccharides (FOS), inulin, and galacto-oligosaccharides (GOS) are widely recognized prebiotics that profoundly affect the intestinal microbiota, including stimulation of bifidobacteria and lactobacilli, and are reported to elicit several health benefits.
34732247	2	55	theme	commensal	496:504	arg1	populations					520:530	commensal Lactobacillus populations	496:530	commensal Lactobacillus populations	496:530	The combination of dietary FOS and inulin with calcium phosphate was reported to stimulate commensal Lactobacillus populations and protect the host against pathogenic Enterobacteriaceae, but little is known about the effects of GOS in diets with a different level of calcium phosphate.
34732247	0	56	theme	microbiome	47:56	arg1	changes					58:64	gut microbiome changes	43:64	gut microbiome changes elicited by inulin and galacto-oligosaccharides consumption	43:124	Dietary calcium phosphate strongly impacts gut microbiome changes elicited by inulin and galacto-oligosaccharides consumption.
34732247	3	57	theme	microbiome	719:728	arg1	changes					730:736	the microbiome changes	715:736	the microbiome changes elicited by dietary supplementation with GOS or inulin	715:791	METHODS We investigated the microbiome changes elicited by dietary supplementation with GOS or inulin using diets with high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats.
34732247	6	58	theme	acid	1459:1462	arg1	levels					1464:1469	increased fecal lactic, succinic, acetic, propionic, and butyric acid levels	1394:1469	increased fecal lactic, succinic, acetic, propionic, and butyric acid levels	1394:1469	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets revealed that diets rich in calcium phosphate favored members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels.
34732247	4	59	theme	bacterial	1079:1087	arg1	gene					1098:1101	the bacterial 16S rRNA gene	1075:1101	the bacterial 16S rRNA gene	1075:1101	Rats were acclimatized to the respective experimental diets for 14 days, after which fecal material was collected, DNA was extracted from fecal material, and the V3‑V4 region of the bacterial 16S rRNA gene was amplified with PCR, followed by microbial composition analysis.
34732247	3	60	theme	dietary	750:756	arg1	supplementation					758:772	dietary supplementation	750:772	dietary supplementation with GOS or inulin	750:791	METHODS We investigated the microbiome changes elicited by dietary supplementation with GOS or inulin using diets with high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats.
34732247	6	61	theme	non-supplemented	1266:1281	arg1	diets					1304:1308	non-supplemented (no prebiotic-added) diets	1266:1308	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets	1245:1308	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets revealed that diets rich in calcium phosphate favored members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels.
34732247	6	62	theme	propionic	1436:1444	arg1	acid					1459:1462	lactic, succinic, acetic, propionic, and butyric acid	1410:1462	increased fecal lactic, succinic, acetic, propionic, and butyric acid levels	1394:1469	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets revealed that diets rich in calcium phosphate favored members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels.
34732247	1	63	theme	intestinal	272:281	arg1	microbiota					283:292	the intestinal microbiota	268:292	the intestinal microbiota	268:292	BACKGROUND Fructo-oligosaccharides (FOS), inulin, and galacto-oligosaccharides (GOS) are widely recognized prebiotics that profoundly affect the intestinal microbiota, including stimulation of bifidobacteria and lactobacilli, and are reported to elicit several health benefits.
34732247	4	64	theme	V3‑V4	1059:1063	arg1	region					1065:1070	the V3‑V4 region	1055:1070	the V3‑V4 region of the bacterial 16S rRNA gene	1055:1101	Rats were acclimatized to the respective experimental diets for 14 days, after which fecal material was collected, DNA was extracted from fecal material, and the V3‑V4 region of the bacterial 16S rRNA gene was amplified with PCR, followed by microbial composition analysis.
34732247	6	65	theme	Feeding	1253:1259	arg1	rats					1261:1264	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets	1245:1308	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets	1245:1308	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets revealed that diets rich in calcium phosphate favored members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels.
34732247	6	66	theme	acetic	1428:1433	arg1	acid					1459:1462	lactic, succinic, acetic, propionic, and butyric acid	1410:1462	increased fecal lactic, succinic, acetic, propionic, and butyric acid levels	1394:1469	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets revealed that diets rich in calcium phosphate favored members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels.
34732247	5	67	theme	organic	1186:1192	arg1	profiles					1199:1206	the organic acid profiles	1182:1206	the organic acid profiles of the fecal material	1182:1228	In tandem, the organic acid profiles of the fecal material were analyzed.
34732247	2	68	theme	GOS	633:635	arg1	effects					622:628	the effects	618:628	the effects of GOS in diets with a different level of calcium phosphate	618:688	The combination of dietary FOS and inulin with calcium phosphate was reported to stimulate commensal Lactobacillus populations and protect the host against pathogenic Enterobacteriaceae, but little is known about the effects of GOS in diets with a different level of calcium phosphate.
34732247	2	69	from	effects	622:628	arg1	diets					640:644	diets	640:644	diets with a different level of calcium phosphate	640:688	The combination of dietary FOS and inulin with calcium phosphate was reported to stimulate commensal Lactobacillus populations and protect the host against pathogenic Enterobacteriaceae, but little is known about the effects of GOS in diets with a different level of calcium phosphate.
34732247	6	70	dep	rats	1261:1264	arg1	diets					1304:1308	non-supplemented (no prebiotic-added) diets	1266:1308	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets	1245:1308	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets revealed that diets rich in calcium phosphate favored members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels.
34732247	2	71	with	diets	640:644	arg1	level					663:667	a different level	651:667	a different level of calcium phosphate	651:688	The combination of dietary FOS and inulin with calcium phosphate was reported to stimulate commensal Lactobacillus populations and protect the host against pathogenic Enterobacteriaceae, but little is known about the effects of GOS in diets with a different level of calcium phosphate.
34732247	3	72	from	levels	868:873	arg1	rats					891:894	adult Wistar rats	878:894	adult Wistar rats	878:894	METHODS We investigated the microbiome changes elicited by dietary supplementation with GOS or inulin using diets with high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats.
34732247	4	73	theme	rRNA	1093:1096	arg1	gene					1098:1101	the bacterial 16S rRNA gene	1075:1101	the bacterial 16S rRNA gene	1075:1101	Rats were acclimatized to the respective experimental diets for 14 days, after which fecal material was collected, DNA was extracted from fecal material, and the V3‑V4 region of the bacterial 16S rRNA gene was amplified with PCR, followed by microbial composition analysis.
34732247	8	74	theme	Faecalibaculum	1909:1922	arg1	populations					1924:1934	the endogenous Faecalibaculum populations	1894:1934	the endogenous Faecalibaculum populations	1894:1934	Irrespective of the calcium phosphate levels, supplementation with GOS or inulin strongly stimulated Bifidobacterium, while only high calcium phosphate diets increased the endogenous Faecalibaculum populations.
34732247	4	75	theme	respective	927:936	arg1	diets					951:955	the respective experimental diets	923:955	the respective experimental diets	923:955	Rats were acclimatized to the respective experimental diets for 14 days, after which fecal material was collected, DNA was extracted from fecal material, and the V3‑V4 region of the bacterial 16S rRNA gene was amplified with PCR, followed by microbial composition analysis.
34732247	8	76	with	supplementation	1772:1786	arg1	inulin					1800:1805	inulin	1800:1805	inulin	1800:1805	Irrespective of the calcium phosphate levels, supplementation with GOS or inulin strongly stimulated Bifidobacterium, while only high calcium phosphate diets increased the endogenous Faecalibaculum populations.
34732247	8	76	with	supplementation	1772:1786	arg1	GOS					1793:1795	GOS	1793:1795	GOS	1793:1795	Irrespective of the calcium phosphate levels, supplementation with GOS or inulin strongly stimulated Bifidobacterium, while only high calcium phosphate diets increased the endogenous Faecalibaculum populations.
34732247	1	77	theme	recognized	223:232	arg1	inulin					169:174	inulin	169:174	inulin	169:174	BACKGROUND Fructo-oligosaccharides (FOS), inulin, and galacto-oligosaccharides (GOS) are widely recognized prebiotics that profoundly affect the intestinal microbiota, including stimulation of bifidobacteria and lactobacilli, and are reported to elicit several health benefits.
34732247	1	77	theme	recognized	223:232	arg1	Fructo-oligosaccharides					138:160	BACKGROUND Fructo-oligosaccharides	127:160	BACKGROUND Fructo-oligosaccharides (FOS)	127:166	BACKGROUND Fructo-oligosaccharides (FOS), inulin, and galacto-oligosaccharides (GOS) are widely recognized prebiotics that profoundly affect the intestinal microbiota, including stimulation of bifidobacteria and lactobacilli, and are reported to elicit several health benefits.
34732247	1	77	theme	recognized	223:232	arg1	galacto-oligosaccharides					181:204	galacto-oligosaccharides	181:204	galacto-oligosaccharides (GOS)	181:210	BACKGROUND Fructo-oligosaccharides (FOS), inulin, and galacto-oligosaccharides (GOS) are widely recognized prebiotics that profoundly affect the intestinal microbiota, including stimulation of bifidobacteria and lactobacilli, and are reported to elicit several health benefits.
34732247	1	77	theme	recognized	223:232	arg1	prebiotics					234:243	widely recognized prebiotics	216:243	widely recognized prebiotics that profoundly affect the intestinal microbiota, including stimulation of bifidobacteria and lactobacilli, and are reported to elicit several health benefits	216:402	BACKGROUND Fructo-oligosaccharides (FOS), inulin, and galacto-oligosaccharides (GOS) are widely recognized prebiotics that profoundly affect the intestinal microbiota, including stimulation of bifidobacteria and lactobacilli, and are reported to elicit several health benefits.
34732247	3	78	with	supplementation	758:772	arg1	inulin					786:791	inulin	786:791	inulin	786:791	METHODS We investigated the microbiome changes elicited by dietary supplementation with GOS or inulin using diets with high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats.
34732247	3	78	with	supplementation	758:772	arg1	GOS					779:781	GOS	779:781	GOS	779:781	METHODS We investigated the microbiome changes elicited by dietary supplementation with GOS or inulin using diets with high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats.
34732247	10	79	theme	diet	2318:2321	arg1	information					2323:2333	detailed diet information	2309:2333	detailed diet information	2309:2333	Therefore, our study implies that the collection of detailed diet information including micronutrient balance is necessary to correctly assess diet-driven microbiota analysis.
34732247	7	80	theme	genera	1575:1580	arg1	abundance					1546:1554	the abundance	1542:1554	the abundance of mucin degrading genera	1542:1580	In contrast, relatively low dietary calcium phosphate levels promoted the abundance of mucin degrading genera like Akkermansia and Bacteroides, and resulted in increased fecal propionic acid levels and modest increases in lactic and butyric acid levels.
34732247	10	81	theme	micronutrient	2345:2357	arg1	balance					2359:2365	micronutrient balance	2345:2365	micronutrient balance	2345:2365	Therefore, our study implies that the collection of detailed diet information including micronutrient balance is necessary to correctly assess diet-driven microbiota analysis.
34732247	3	82	theme	adult	878:882	arg1	rats					891:894	adult Wistar rats	878:894	adult Wistar rats	878:894	METHODS We investigated the microbiome changes elicited by dietary supplementation with GOS or inulin using diets with high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats.
34732247	7	83	theme	mucin	1559:1563	arg1	genera					1575:1580	mucin degrading genera	1559:1580	mucin degrading genera	1559:1580	In contrast, relatively low dietary calcium phosphate levels promoted the abundance of mucin degrading genera like Akkermansia and Bacteroides, and resulted in increased fecal propionic acid levels and modest increases in lactic and butyric acid levels.
34732247	7	84	from	levels	1663:1668	arg1	levels					1718:1723	lactic and butyric acid levels	1694:1723	lactic and butyric acid levels	1694:1723	In contrast, relatively low dietary calcium phosphate levels promoted the abundance of mucin degrading genera like Akkermansia and Bacteroides, and resulted in increased fecal propionic acid levels and modest increases in lactic and butyric acid levels.
34732247	9	85	theme	chemical	1999:2006	arg1	structure					2008:2016	chemical structure	1999:2016	chemical structure	1999:2016	CONCLUSIONS Despite the prebiotic's substantial difference in chemical structure, sugar composition, oligomer size, and the microbial degradation pathway involved in their utilization, inulin and GOS modulated the gut microbiota very similarly, in a manner that strongly depended on the dietary calcium phosphate level.
34732247	9	86	theme	dietary	2224:2230	arg1	level					2250:2254	the dietary calcium phosphate level	2220:2254	the dietary calcium phosphate level	2220:2254	CONCLUSIONS Despite the prebiotic's substantial difference in chemical structure, sugar composition, oligomer size, and the microbial degradation pathway involved in their utilization, inulin and GOS modulated the gut microbiota very similarly, in a manner that strongly depended on the dietary calcium phosphate level.
34732247	8	87	theme	phosphate	1754:1762	arg1	levels					1764:1769	the calcium phosphate levels	1742:1769	the calcium phosphate levels	1742:1769	Irrespective of the calcium phosphate levels, supplementation with GOS or inulin strongly stimulated Bifidobacterium, while only high calcium phosphate diets increased the endogenous Faecalibaculum populations.
34732247	7	88	theme	acid	1713:1716	arg1	levels					1718:1723	lactic and butyric acid levels	1694:1723	lactic and butyric acid levels	1694:1723	In contrast, relatively low dietary calcium phosphate levels promoted the abundance of mucin degrading genera like Akkermansia and Bacteroides, and resulted in increased fecal propionic acid levels and modest increases in lactic and butyric acid levels.
34732247	10	89	theme	diet-driven	2400:2410	arg1	analysis					2423:2430	diet-driven microbiota analysis	2400:2430	diet-driven microbiota analysis	2400:2430	Therefore, our study implies that the collection of detailed diet information including micronutrient balance is necessary to correctly assess diet-driven microbiota analysis.
34732247	4	90	theme	composition	1149:1159	arg1	analysis					1161:1168	microbial composition analysis	1139:1168	microbial composition analysis	1139:1168	Rats were acclimatized to the respective experimental diets for 14 days, after which fecal material was collected, DNA was extracted from fecal material, and the V3‑V4 region of the bacterial 16S rRNA gene was amplified with PCR, followed by microbial composition analysis.
34732247	5	91	theme	material	1221:1228	arg1	profiles					1199:1206	the organic acid profiles	1182:1206	the organic acid profiles of the fecal material	1182:1228	In tandem, the organic acid profiles of the fecal material were analyzed.
34732247	9	92	theme	phosphate	2240:2248	arg1	level					2250:2254	the dietary calcium phosphate level	2220:2254	the dietary calcium phosphate level	2220:2254	CONCLUSIONS Despite the prebiotic's substantial difference in chemical structure, sugar composition, oligomer size, and the microbial degradation pathway involved in their utilization, inulin and GOS modulated the gut microbiota very similarly, in a manner that strongly depended on the dietary calcium phosphate level.
34732247	4	93	dep	amplified	1107:1115	arg1	followed					1127:1134	followed	1127:1134	followed by microbial composition analysis	1127:1168	Rats were acclimatized to the respective experimental diets for 14 days, after which fecal material was collected, DNA was extracted from fecal material, and the V3‑V4 region of the bacterial 16S rRNA gene was amplified with PCR, followed by microbial composition analysis.
34732247	1	94	theme	health	388:393	arg1	benefits					395:402	several health benefits	380:402	several health benefits	380:402	BACKGROUND Fructo-oligosaccharides (FOS), inulin, and galacto-oligosaccharides (GOS) are widely recognized prebiotics that profoundly affect the intestinal microbiota, including stimulation of bifidobacteria and lactobacilli, and are reported to elicit several health benefits.
34732247	3	95	theme	calcium	850:856	arg1	levels					868:873	high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels	810:873	high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats	810:894	METHODS We investigated the microbiome changes elicited by dietary supplementation with GOS or inulin using diets with high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats.
34732247	1	96	theme	several	380:386	arg1	benefits					395:402	several health benefits	380:402	several health benefits	380:402	BACKGROUND Fructo-oligosaccharides (FOS), inulin, and galacto-oligosaccharides (GOS) are widely recognized prebiotics that profoundly affect the intestinal microbiota, including stimulation of bifidobacteria and lactobacilli, and are reported to elicit several health benefits.
34732247	9	97	theme	oligomer	2038:2045	arg1	size					2047:2050	oligomer size	2038:2050	oligomer size	2038:2050	CONCLUSIONS Despite the prebiotic's substantial difference in chemical structure, sugar composition, oligomer size, and the microbial degradation pathway involved in their utilization, inulin and GOS modulated the gut microbiota very similarly, in a manner that strongly depended on the dietary calcium phosphate level.
34732247	7	98	theme	phosphate	1516:1524	arg1	levels					1526:1531	relatively low dietary calcium phosphate levels	1485:1531	relatively low dietary calcium phosphate levels	1485:1531	In contrast, relatively low dietary calcium phosphate levels promoted the abundance of mucin degrading genera like Akkermansia and Bacteroides, and resulted in increased fecal propionic acid levels and modest increases in lactic and butyric acid levels.
34732247	2	99	theme	phosphate	680:688	arg1	level					663:667	a different level	651:667	a different level of calcium phosphate	651:688	The combination of dietary FOS and inulin with calcium phosphate was reported to stimulate commensal Lactobacillus populations and protect the host against pathogenic Enterobacteriaceae, but little is known about the effects of GOS in diets with a different level of calcium phosphate.
34732247	6	100	from	rich	1330:1333	arg1	phosphate					1346:1354	calcium phosphate	1338:1354	calcium phosphate	1338:1354	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets revealed that diets rich in calcium phosphate favored members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels.
34732247	7	101	theme	modest	1674:1679	arg1	increases					1681:1689	modest increases	1674:1689	modest increases in lactic and butyric acid levels	1674:1723	In contrast, relatively low dietary calcium phosphate levels promoted the abundance of mucin degrading genera like Akkermansia and Bacteroides, and resulted in increased fecal propionic acid levels and modest increases in lactic and butyric acid levels.
34732247	2	102	theme	dietary	424:430	arg1	FOS					432:434	dietary FOS	424:434	dietary FOS	424:434	The combination of dietary FOS and inulin with calcium phosphate was reported to stimulate commensal Lactobacillus populations and protect the host against pathogenic Enterobacteriaceae, but little is known about the effects of GOS in diets with a different level of calcium phosphate.
34732247	0	103	theme	calcium	8:14	arg1	phosphate					16:24	Dietary calcium phosphate	0:24	Dietary calcium phosphate	0:24	Dietary calcium phosphate strongly impacts gut microbiome changes elicited by inulin and galacto-oligosaccharides consumption.
34732247	7	104	theme	propionic	1648:1656	arg1	acid					1658:1661	fecal propionic acid	1642:1661	increased fecal propionic acid levels	1632:1668	In contrast, relatively low dietary calcium phosphate levels promoted the abundance of mucin degrading genera like Akkermansia and Bacteroides, and resulted in increased fecal propionic acid levels and modest increases in lactic and butyric acid levels.
34732247	9	105	from	difference	1985:1994	arg1	structure					2008:2016	chemical structure	1999:2016	chemical structure	1999:2016	CONCLUSIONS Despite the prebiotic's substantial difference in chemical structure, sugar composition, oligomer size, and the microbial degradation pathway involved in their utilization, inulin and GOS modulated the gut microbiota very similarly, in a manner that strongly depended on the dietary calcium phosphate level.
34732247	9	105	from	difference	1985:1994	arg1	composition					2025:2035	sugar composition	2019:2035	sugar composition	2019:2035	CONCLUSIONS Despite the prebiotic's substantial difference in chemical structure, sugar composition, oligomer size, and the microbial degradation pathway involved in their utilization, inulin and GOS modulated the gut microbiota very similarly, in a manner that strongly depended on the dietary calcium phosphate level.
34732247	9	105	from	difference	1985:1994	arg1	size					2047:2050	oligomer size	2038:2050	oligomer size	2038:2050	CONCLUSIONS Despite the prebiotic's substantial difference in chemical structure, sugar composition, oligomer size, and the microbial degradation pathway involved in their utilization, inulin and GOS modulated the gut microbiota very similarly, in a manner that strongly depended on the dietary calcium phosphate level.
34732247	9	105	from	difference	1985:1994	arg1	pathway					2083:2089	the microbial degradation pathway	2057:2089	the microbial degradation pathway involved in their utilization, inulin and GOS	2057:2135	CONCLUSIONS Despite the prebiotic's substantial difference in chemical structure, sugar composition, oligomer size, and the microbial degradation pathway involved in their utilization, inulin and GOS modulated the gut microbiota very similarly, in a manner that strongly depended on the dietary calcium phosphate level.
34732247	4	106	theme	fecal	1035:1039	arg1	material					1041:1048	fecal material	1035:1048	fecal material	1035:1048	Rats were acclimatized to the respective experimental diets for 14 days, after which fecal material was collected, DNA was extracted from fecal material, and the V3‑V4 region of the bacterial 16S rRNA gene was amplified with PCR, followed by microbial composition analysis.
34732247	9	107	theme	degradation	2071:2081	arg1	pathway					2083:2089	the microbial degradation pathway	2057:2089	the microbial degradation pathway involved in their utilization, inulin and GOS	2057:2135	CONCLUSIONS Despite the prebiotic's substantial difference in chemical structure, sugar composition, oligomer size, and the microbial degradation pathway involved in their utilization, inulin and GOS modulated the gut microbiota very similarly, in a manner that strongly depended on the dietary calcium phosphate level.
34732247	6	108	theme	fecal	1404:1408	arg1	levels					1464:1469	increased fecal lactic, succinic, acetic, propionic, and butyric acid levels	1394:1469	increased fecal lactic, succinic, acetic, propionic, and butyric acid levels	1394:1469	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets revealed that diets rich in calcium phosphate favored members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels.
34732247	7	109	theme	calcium	1508:1514	arg1	levels					1526:1531	relatively low dietary calcium phosphate levels	1485:1531	relatively low dietary calcium phosphate levels	1485:1531	In contrast, relatively low dietary calcium phosphate levels promoted the abundance of mucin degrading genera like Akkermansia and Bacteroides, and resulted in increased fecal propionic acid levels and modest increases in lactic and butyric acid levels.
34732247	7	110	theme	increased	1632:1640	arg1	levels					1663:1668	increased fecal propionic acid levels	1632:1668	increased fecal propionic acid levels	1632:1668	In contrast, relatively low dietary calcium phosphate levels promoted the abundance of mucin degrading genera like Akkermansia and Bacteroides, and resulted in increased fecal propionic acid levels and modest increases in lactic and butyric acid levels.
34732247	2	111	theme	Lactobacillus	506:518	arg1	populations					520:530	commensal Lactobacillus populations	496:530	commensal Lactobacillus populations	496:530	The combination of dietary FOS and inulin with calcium phosphate was reported to stimulate commensal Lactobacillus populations and protect the host against pathogenic Enterobacteriaceae, but little is known about the effects of GOS in diets with a different level of calcium phosphate.
34732247	7	112	theme	low	1496:1498	arg1	levels					1526:1531	relatively low dietary calcium phosphate levels	1485:1531	relatively low dietary calcium phosphate levels	1485:1531	In contrast, relatively low dietary calcium phosphate levels promoted the abundance of mucin degrading genera like Akkermansia and Bacteroides, and resulted in increased fecal propionic acid levels and modest increases in lactic and butyric acid levels.
34732247	6	113	theme	levels	1464:1469	arg1	members					1364:1370	members	1364:1370	members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels	1364:1469	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets revealed that diets rich in calcium phosphate favored members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels.
34732247	6	114	theme	butyric	1451:1457	arg1	acid					1459:1462	lactic, succinic, acetic, propionic, and butyric acid	1410:1462	increased fecal lactic, succinic, acetic, propionic, and butyric acid levels	1394:1469	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets revealed that diets rich in calcium phosphate favored members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels.
34732247	6	115	theme	Firmicutes	1379:1388	arg1	members					1364:1370	members	1364:1370	members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels	1364:1469	RESULTS Feeding rats non-supplemented (no prebiotic-added) diets revealed that diets rich in calcium phosphate favored members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels.
34732247	3	116	dep	METHODS	691:697	arg1	investigated					702:713	investigated	702:713	investigated the microbiome changes elicited by dietary supplementation with GOS or inulin using diets with high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats	702:894	METHODS We investigated the microbiome changes elicited by dietary supplementation with GOS or inulin using diets with high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats.
34732247	5	117	theme	fecal	1215:1219	arg1	material					1221:1228	the fecal material	1211:1228	the fecal material	1211:1228	In tandem, the organic acid profiles of the fecal material were analyzed.
32527472	5	0	theme	structure	718:726	arg1	maintenance					691:701	the maintenance	687:701	the maintenance of the spatial structure	687:726	After decellularization, we get a scaffold made of a variety of proteins, and it is the role of these proteins that can significantly affect the maintenance of the spatial structure and be a suitable environment for cells to rebuild a specific organ.
32527472	11	1	theme	DNA	1320:1322	arg1	concentration					1324:1336	the final DNA concentration	1310:1336	the final DNA concentration	1310:1336	After decellularization, an assessment of the final DNA concentration and the protein composition was performed.
32527472	6	2	theme	detergent	819:827	arg1	parameters					849:858	detergent (Triton X-100) flow parameters	819:858	detergent (Triton X-100) flow parameters	819:858	AIM Estimation of the detergent (Triton X-100) flow parameters and anthropometric donors' decellularization process accuracy on the final ECM composition.
32527472	1	3	theme	extracellular	162:174	arg1	ECM					184:186	ECM	184:186	ECM	184:186	INTRODUCTION The extracellular matrix (ECM) consists, among others, of polysaccharides, glycosaminoglycans, and proteins.
32527472	1	3	theme	extracellular	162:174	arg1	matrix					176:181	The extracellular matrix	158:181	INTRODUCTION The extracellular matrix (ECM)	145:187	INTRODUCTION The extracellular matrix (ECM) consists, among others, of polysaccharides, glycosaminoglycans, and proteins.
32527472	16	4	theme	protein	1676:1682	arg1	profile					1684:1690	the protein profile	1672:1690	the protein profile with anthropometric data	1672:1715	Analysis of the protein profile with anthropometric data has shown that LAM A2 was increasing with age and LAMA5 was decreasing.
32527472	11	5	theme	concentration	1324:1336	arg1	assessment					1296:1305	an assessment	1293:1305	an assessment of the final DNA concentration and the protein composition	1293:1364	After decellularization, an assessment of the final DNA concentration and the protein composition was performed.
32527472	4	6	theme	enzymatic	503:511	arg1	methods					528:534	chemical, enzymatic, or mechanical methods	493:534	chemical, enzymatic, or mechanical methods	493:534	To deprive organs of the cellular part, chemical, enzymatic, or mechanical methods are used.
32527472	14	7	theme	best	1507:1510	arg1	results					1512:1518	The best results	1503:1518	RESULTS The best results	1495:1518	RESULTS The best results were obtained using a flow of 120 mL/minute.
32527472	6	8	theme	AIM	797:799	arg1	Estimation					801:810	AIM Estimation	797:810	AIM Estimation of the detergent (Triton X-100) flow parameters and anthropometric donors' decellularization process	797:911	AIM Estimation of the detergent (Triton X-100) flow parameters and anthropometric donors' decellularization process accuracy on the final ECM composition.
32527472	3	9	theme	quality	352:358	arg1	ECM					333:335	ECM	333:335	ECM of the highest quality	333:358	Obtaining ECM of the highest quality through decellularization is a big challenge because of some differences in organ structure.
32527472	8	10	dep	years	1110:1114	arg1	to					1104:1105	to	1104:1105	to	1104:1105	All organs were harvested from brain-dead donors age 13 to 60 years.
32527472	14	11	theme	mL/minute	1554:1562	arg1	flow					1542:1545	a flow	1540:1545	a flow of 120 mL/minute	1540:1562	RESULTS The best results were obtained using a flow of 120 mL/minute.
32527472	9	12	theme	METHODS	1117:1123	arg1	Decellularization					1125:1141	METHODS Decellularization	1117:1141	METHODS Decellularization	1117:1141	METHODS Decellularization was carried out using the flow method with Triton X-100 as an active agent.
32527472	6	13	theme	ECM	935:937	arg1	composition					939:949	the final ECM composition	925:949	the final ECM composition	925:949	AIM Estimation of the detergent (Triton X-100) flow parameters and anthropometric donors' decellularization process accuracy on the final ECM composition.
32527472	0	14	theme	Extracellular	72:84	arg1	Composition					93:103	the Extracellular Matrix Composition	68:103	the Extracellular Matrix Composition After Human Pancreas Decellularization	68:142	The Influence of the Flow of Detergent and Donor Characteristics on the Extracellular Matrix Composition After Human Pancreas Decellularization.
32527472	16	15	theme	profile	1684:1690	arg1	Analysis					1660:1667	Analysis	1660:1667	Analysis of the protein profile with anthropometric data	1660:1715	Analysis of the protein profile with anthropometric data has shown that LAM A2 was increasing with age and LAMA5 was decreasing.
32527472	4	16	used	used	540:543	arg2	methods					528:534	chemical, enzymatic, or mechanical methods	493:534	chemical, enzymatic, or mechanical methods	493:534	To deprive organs of the cellular part, chemical, enzymatic, or mechanical methods are used.
32527472	12	17	theme	anthropometric	1406:1419	arg1	data					1421:1424	anthropometric data	1406:1424	anthropometric data of donors	1406:1434	Results were compared to anthropometric data of donors.
32527472	7	18	dep	MATERIALS	952:960	arg1	pancreata					973:981	Five human pancreata	962:981	MATERIALS Five human pancreata	952:981	MATERIALS Five human pancreata, rejected from transplantation, were used for decellularization.
32527472	6	19	theme	final	929:933	arg1	composition					939:949	the final ECM composition	925:949	the final ECM composition	925:949	AIM Estimation of the detergent (Triton X-100) flow parameters and anthropometric donors' decellularization process accuracy on the final ECM composition.
32527472	11	20	theme	protein	1346:1352	arg1	composition					1354:1364	the protein composition	1342:1364	the protein composition	1342:1364	After decellularization, an assessment of the final DNA concentration and the protein composition was performed.
32527472	6	21	theme	Triton	830:835	arg1	detergent					819:827	detergent	819:827	detergent (Triton X-100) flow parameters	819:858	AIM Estimation of the detergent (Triton X-100) flow parameters and anthropometric donors' decellularization process accuracy on the final ECM composition.
32527472	6	21	theme	Triton	830:835	arg1	X-100					837:841	Triton X-100	830:841	Triton X-100	830:841	AIM Estimation of the detergent (Triton X-100) flow parameters and anthropometric donors' decellularization process accuracy on the final ECM composition.
32527472	15	22	theme	residual	1634:1641	arg1	DNA					1643:1645	residual DNA	1634:1645	residual DNA	1634:1645	A higher detergent flow was associated with a lower concentration of residual DNA in scaffold.
32527472	3	23	theme	organ	436:440	arg1	structure					442:450	organ structure	436:450	organ structure	436:450	Obtaining ECM of the highest quality through decellularization is a big challenge because of some differences in organ structure.
32527472	3	24	theme	highest	344:350	arg1	quality					352:358	the highest quality	340:358	the highest quality	340:358	Obtaining ECM of the highest quality through decellularization is a big challenge because of some differences in organ structure.
32527472	4	25	theme	part	487:490	arg1	organs					464:469	organs	464:469	organs of the cellular part	464:490	To deprive organs of the cellular part, chemical, enzymatic, or mechanical methods are used.
32527472	14	26	dep	RESULTS	1495:1501	arg1	results					1512:1518	The best results	1503:1518	RESULTS The best results	1495:1518	RESULTS The best results were obtained using a flow of 120 mL/minute.
32527472	3	27	theme	big	391:393	arg1	challenge					395:403	a big challenge	389:403	a big challenge	389:403	Obtaining ECM of the highest quality through decellularization is a big challenge because of some differences in organ structure.
32527472	4	28	theme	cellular	478:485	arg1	part					487:490	the cellular part	474:490	the cellular part	474:490	To deprive organs of the cellular part, chemical, enzymatic, or mechanical methods are used.
32527472	17	29	theme	higher	1828:1833	arg1	proportion					1835:1844	a higher proportion	1826:1844	a higher proportion of COL1 and 4	1826:1858	Being overweight was associated with a higher proportion of COL1 and 4 and a smaller proportion of COL6.
32527472	8	30	theme	age	1097:1099	arg1	years					1110:1114	age 13 to 60 years	1097:1114	brain-dead donors age 13 to 60 years	1079:1114	All organs were harvested from brain-dead donors age 13 to 60 years.
32527472	13	31	theme	microscopic	1452:1462	arg1	analysis					1464:1471	a microscopic analysis	1450:1471	a microscopic analysis	1450:1471	In addition, a microscopic analysis was also carried out.
32527472	0	32	from	Influence	4:12	arg1	Composition					93:103	the Extracellular Matrix Composition	68:103	the Extracellular Matrix Composition After Human Pancreas Decellularization	68:142	The Influence of the Flow of Detergent and Donor Characteristics on the Extracellular Matrix Composition After Human Pancreas Decellularization.
32527472	5	33	theme	proteins	610:617	arg1	proteins					610:617	proteins	610:617	proteins	610:617	After decellularization, we get a scaffold made of a variety of proteins, and it is the role of these proteins that can significantly affect the maintenance of the spatial structure and be a suitable environment for cells to rebuild a specific organ.
32527472	5	33	theme	proteins	610:617	arg1	variety					599:605	a variety	597:605	a variety of proteins	597:617	After decellularization, we get a scaffold made of a variety of proteins, and it is the role of these proteins that can significantly affect the maintenance of the spatial structure and be a suitable environment for cells to rebuild a specific organ.
32527472	9	34	theme	flow	1169:1172	arg1	agent					1212:1216	an active agent	1202:1216	an active agent	1202:1216	METHODS Decellularization was carried out using the flow method with Triton X-100 as an active agent.
32527472	9	34	theme	flow	1169:1172	arg1	method					1174:1179	the flow method	1165:1179	the flow method with Triton X-100	1165:1197	METHODS Decellularization was carried out using the flow method with Triton X-100 as an active agent.
32527472	2	35	theme	tissue	300:305	arg1	bioengineering					307:320	tissue bioengineering	300:320	tissue bioengineering	300:320	It is being increasingly used in tissue bioengineering.
32527472	9	36	with	method	1174:1179	arg1	X-100					1193:1197	Triton X-100	1186:1197	Triton X-100	1186:1197	METHODS Decellularization was carried out using the flow method with Triton X-100 as an active agent.
32527472	0	37	theme	Pancreas	117:124	arg1	Decellularization					126:142	Human Pancreas Decellularization	111:142	Human Pancreas Decellularization	111:142	The Influence of the Flow of Detergent and Donor Characteristics on the Extracellular Matrix Composition After Human Pancreas Decellularization.
32527472	1	38	dep	INTRODUCTION	145:156	arg1	ECM					184:186	ECM	184:186	ECM	184:186	INTRODUCTION The extracellular matrix (ECM) consists, among others, of polysaccharides, glycosaminoglycans, and proteins.
32527472	1	38	dep	INTRODUCTION	145:156	arg1	matrix					176:181	The extracellular matrix	158:181	INTRODUCTION The extracellular matrix (ECM)	145:187	INTRODUCTION The extracellular matrix (ECM) consists, among others, of polysaccharides, glycosaminoglycans, and proteins.
32527472	15	39	theme	DNA	1643:1645	arg1	concentration					1617:1629	a lower concentration	1609:1629	a lower concentration of residual DNA in scaffold	1609:1657	A higher detergent flow was associated with a lower concentration of residual DNA in scaffold.
32527472	7	40	used	used	1020:1023	arg2	MATERIALS					952:960	MATERIALS	952:960	MATERIALS Five human pancreata	952:981	MATERIALS Five human pancreata, rejected from transplantation, were used for decellularization.
32527472	16	41	dep	LAM	1732:1734	arg1	A2					1736:1737	A2	1736:1737	LAM A2	1732:1737	Analysis of the protein profile with anthropometric data has shown that LAM A2 was increasing with age and LAMA5 was decreasing.
32527472	0	42	theme	Human	111:115	arg1	Decellularization					126:142	Human Pancreas Decellularization	111:142	Human Pancreas Decellularization	111:142	The Influence of the Flow of Detergent and Donor Characteristics on the Extracellular Matrix Composition After Human Pancreas Decellularization.
32527472	10	43	theme	flow	1255:1258	arg1	values					1260:1265	5 different flow values	1243:1265	5 different flow values	1243:1265	The experiment compared 5 different flow values.
32527472	5	44	theme	specific	781:788	arg1	organ					790:794	a specific organ	779:794	a specific organ	779:794	After decellularization, we get a scaffold made of a variety of proteins, and it is the role of these proteins that can significantly affect the maintenance of the spatial structure and be a suitable environment for cells to rebuild a specific organ.
32527472	6	45	theme	process	905:911	arg1	Estimation					801:810	AIM Estimation	797:810	AIM Estimation of the detergent (Triton X-100) flow parameters and anthropometric donors' decellularization process	797:911	AIM Estimation of the detergent (Triton X-100) flow parameters and anthropometric donors' decellularization process accuracy on the final ECM composition.
32527472	9	46	theme	Triton	1186:1191	arg1	X-100					1193:1197	Triton X-100	1186:1197	Triton X-100	1186:1197	METHODS Decellularization was carried out using the flow method with Triton X-100 as an active agent.
32527472	17	47	theme	COL6	1888:1891	arg1	proportion					1874:1883	a smaller proportion	1864:1883	a smaller proportion of COL6	1864:1891	Being overweight was associated with a higher proportion of COL1 and 4 and a smaller proportion of COL6.
32527472	17	47	theme	COL6	1888:1891	arg1	proportion					1835:1844	a higher proportion	1826:1844	a higher proportion of COL1 and 4	1826:1858	Being overweight was associated with a higher proportion of COL1 and 4 and a smaller proportion of COL6.
32527472	10	48	theme	different	1245:1253	arg1	values					1260:1265	5 different flow values	1243:1265	5 different flow values	1243:1265	The experiment compared 5 different flow values.
32527472	0	49	theme	Flow	21:24	arg1	Influence					4:12	The Influence	0:12	The Influence of the Flow of Detergent and Donor Characteristics on the Extracellular Matrix Composition After Human Pancreas Decellularization.	0:143	The Influence of the Flow of Detergent and Donor Characteristics on the Extracellular Matrix Composition After Human Pancreas Decellularization.
32527472	6	50	theme	decellularization	887:903	arg1	process					905:911	the detergent (Triton X-100) flow parameters and anthropometric donors' decellularization process	815:911	the detergent (Triton X-100) flow parameters and anthropometric donors' decellularization process	815:911	AIM Estimation of the detergent (Triton X-100) flow parameters and anthropometric donors' decellularization process accuracy on the final ECM composition.
32527472	11	51	theme	final	1314:1318	arg1	concentration					1324:1336	the final DNA concentration	1310:1336	the final DNA concentration	1310:1336	After decellularization, an assessment of the final DNA concentration and the protein composition was performed.
32527472	11	52	theme	composition	1354:1364	arg1	assessment					1296:1305	an assessment	1293:1305	an assessment of the final DNA concentration and the protein composition	1293:1364	After decellularization, an assessment of the final DNA concentration and the protein composition was performed.
32527472	7	53	theme	human	967:971	arg1	pancreata					973:981	Five human pancreata	962:981	MATERIALS Five human pancreata	952:981	MATERIALS Five human pancreata, rejected from transplantation, were used for decellularization.
32527472	6	54	theme	anthropometric	864:877	arg1	donors					879:884	anthropometric donors	864:884	anthropometric donors	864:884	AIM Estimation of the detergent (Triton X-100) flow parameters and anthropometric donors' decellularization process accuracy on the final ECM composition.
32527472	5	55	theme	suitable	737:744	arg1	environment					746:756	a suitable environment	735:756	a suitable environment for cells to rebuild a specific organ	735:794	After decellularization, we get a scaffold made of a variety of proteins, and it is the role of these proteins that can significantly affect the maintenance of the spatial structure and be a suitable environment for cells to rebuild a specific organ.
32527472	0	56	theme	Matrix	86:91	arg1	Composition					93:103	the Extracellular Matrix Composition	68:103	the Extracellular Matrix Composition After Human Pancreas Decellularization	68:142	The Influence of the Flow of Detergent and Donor Characteristics on the Extracellular Matrix Composition After Human Pancreas Decellularization.
32527472	17	57	theme	COL1	1849:1852	arg1	proportion					1874:1883	a smaller proportion	1864:1883	a smaller proportion of COL6	1864:1891	Being overweight was associated with a higher proportion of COL1 and 4 and a smaller proportion of COL6.
32527472	17	57	theme	COL1	1849:1852	arg1	proportion					1835:1844	a higher proportion	1826:1844	a higher proportion of COL1 and 4	1826:1858	Being overweight was associated with a higher proportion of COL1 and 4 and a smaller proportion of COL6.
32527472	5	58	theme	spatial	710:716	arg1	structure					718:726	the spatial structure	706:726	the spatial structure	706:726	After decellularization, we get a scaffold made of a variety of proteins, and it is the role of these proteins that can significantly affect the maintenance of the spatial structure and be a suitable environment for cells to rebuild a specific organ.
32527472	0	59	theme	Detergent	29:37	arg1	Flow					21:24	the Flow	17:24	the Flow of Detergent and Donor Characteristics	17:63	The Influence of the Flow of Detergent and Donor Characteristics on the Extracellular Matrix Composition After Human Pancreas Decellularization.
32527472	16	60	theme	anthropometric	1697:1710	arg1	data					1712:1715	anthropometric data	1697:1715	anthropometric data	1697:1715	Analysis of the protein profile with anthropometric data has shown that LAM A2 was increasing with age and LAMA5 was decreasing.
32527472	5	61	theme	proteins	648:655	arg1	it					624:625	it	624:625	it	624:625	After decellularization, we get a scaffold made of a variety of proteins, and it is the role of these proteins that can significantly affect the maintenance of the spatial structure and be a suitable environment for cells to rebuild a specific organ.
32527472	5	61	theme	proteins	648:655	arg1	role					634:637	the role	630:637	the role of these proteins that can significantly affect the maintenance of the spatial structure and be a suitable environment for cells to rebuild a specific organ	630:794	After decellularization, we get a scaffold made of a variety of proteins, and it is the role of these proteins that can significantly affect the maintenance of the spatial structure and be a suitable environment for cells to rebuild a specific organ.
32527472	6	62	theme	flow	844:847	arg1	parameters					849:858	detergent (Triton X-100) flow parameters	819:858	detergent (Triton X-100) flow parameters	819:858	AIM Estimation of the detergent (Triton X-100) flow parameters and anthropometric donors' decellularization process accuracy on the final ECM composition.
32527472	12	63	theme	donors	1429:1434	arg1	data					1421:1424	anthropometric data	1406:1424	anthropometric data of donors	1406:1434	Results were compared to anthropometric data of donors.
32527472	15	64	theme	detergent	1574:1582	arg1	flow					1584:1587	A higher detergent flow	1565:1587	A higher detergent flow	1565:1587	A higher detergent flow was associated with a lower concentration of residual DNA in scaffold.
32527472	2	65	used	used	292:295	arg2	It					267:268	It	267:268	It	267:268	It is being increasingly used in tissue bioengineering.
32527472	4	66	theme	mechanical	517:526	arg1	methods					528:534	chemical, enzymatic, or mechanical methods	493:534	chemical, enzymatic, or mechanical methods	493:534	To deprive organs of the cellular part, chemical, enzymatic, or mechanical methods are used.
32527472	8	67	theme	brain-dead	1079:1088	arg1	donors					1090:1095	brain-dead donors	1079:1095	brain-dead donors age 13 to 60 years	1079:1114	All organs were harvested from brain-dead donors age 13 to 60 years.
32527472	9	68	theme	active	1205:1210	arg1	agent					1212:1216	an active agent	1202:1216	an active agent	1202:1216	METHODS Decellularization was carried out using the flow method with Triton X-100 as an active agent.
32527472	9	68	theme	active	1205:1210	arg1	method					1174:1179	the flow method	1165:1179	the flow method with Triton X-100	1165:1197	METHODS Decellularization was carried out using the flow method with Triton X-100 as an active agent.
32527472	15	69	from	concentration	1617:1629	arg1	scaffold					1650:1657	scaffold	1650:1657	scaffold	1650:1657	A higher detergent flow was associated with a lower concentration of residual DNA in scaffold.
32527472	15	70	theme	lower	1611:1615	arg1	concentration					1617:1629	a lower concentration	1609:1629	a lower concentration of residual DNA in scaffold	1609:1657	A higher detergent flow was associated with a lower concentration of residual DNA in scaffold.
32527472	3	71	from	differences	421:431	arg1	structure					442:450	organ structure	436:450	organ structure	436:450	Obtaining ECM of the highest quality through decellularization is a big challenge because of some differences in organ structure.
32527472	8	72	dep	donors	1090:1095	arg1	years					1110:1114	age 13 to 60 years	1097:1114	brain-dead donors age 13 to 60 years	1079:1114	All organs were harvested from brain-dead donors age 13 to 60 years.
32527472	0	73	theme	Characteristics	49:63	arg1	Flow					21:24	the Flow	17:24	the Flow of Detergent and Donor Characteristics	17:63	The Influence of the Flow of Detergent and Donor Characteristics on the Extracellular Matrix Composition After Human Pancreas Decellularization.
32527472	15	74	theme	higher	1567:1572	arg1	flow					1584:1587	A higher detergent flow	1565:1587	A higher detergent flow	1565:1587	A higher detergent flow was associated with a lower concentration of residual DNA in scaffold.
32527472	17	75	theme	smaller	1866:1872	arg1	proportion					1874:1883	a smaller proportion	1864:1883	a smaller proportion of COL6	1864:1891	Being overweight was associated with a higher proportion of COL1 and 4 and a smaller proportion of COL6.
32527472	0	76	theme	Donor	43:47	arg1	Characteristics					49:63	Donor Characteristics	43:63	Donor Characteristics	43:63	The Influence of the Flow of Detergent and Donor Characteristics on the Extracellular Matrix Composition After Human Pancreas Decellularization.
32527472	4	77	theme	chemical	493:500	arg1	methods					528:534	chemical, enzymatic, or mechanical methods	493:534	chemical, enzymatic, or mechanical methods	493:534	To deprive organs of the cellular part, chemical, enzymatic, or mechanical methods are used.
32527472	17	78	theme	4	1858:1858	arg1	proportion					1874:1883	a smaller proportion	1864:1883	a smaller proportion of COL6	1864:1891	Being overweight was associated with a higher proportion of COL1 and 4 and a smaller proportion of COL6.
32527472	17	78	theme	4	1858:1858	arg1	proportion					1835:1844	a higher proportion	1826:1844	a higher proportion of COL1 and 4	1826:1858	Being overweight was associated with a higher proportion of COL1 and 4 and a smaller proportion of COL6.
32527472	16	79	with	profile	1684:1690	arg1	data					1712:1715	anthropometric data	1697:1715	anthropometric data	1697:1715	Analysis of the protein profile with anthropometric data has shown that LAM A2 was increasing with age and LAMA5 was decreasing.
31985269	4	0	theme	orthopaedic	527:537	arg1	infections					539:548	orthopaedic infections	527:548	orthopaedic infections	527:548	Here, twenty-two S. lugdunensis clinical isolates, mainly from orthopaedic infections but also from other clinical sources, were sub-grouped by ribotyping and dendrogram analysis.
31985269	7	1	theme	intriguing	942:951	arg1	diversities					953:963	intriguing diversities	942:963	intriguing diversities	942:963	Isolates displayed intriguing diversities in biofilm mass and matrix composition.
31985269	4	2	from	sources	579:585	arg1	isolates					505:512	twenty-two S. lugdunensis clinical isolates	470:512	twenty-two S. lugdunensis clinical isolates	470:512	Here, twenty-two S. lugdunensis clinical isolates, mainly from orthopaedic infections but also from other clinical sources, were sub-grouped by ribotyping and dendrogram analysis.
31985269	5	3	theme	FITC-Wheat	700:709	arg1	Agglutinin					716:725	FITC-Wheat Germ Agglutinin	700:725	FITC-Wheat Germ Agglutinin	700:725	Biofilms were analysed by fluorimetric methods based on FITC-Wheat Germ Agglutinin, SYPRO Ruby and TOTO-1 dyes to detect exopolysaccharides, proteins and extracellular DNA (eDNA), respectively.
31985269	4	4	theme	ribotyping	608:617	arg1	analysis					634:641	ribotyping and dendrogram analysis	608:641	analysis	634:641	Here, twenty-two S. lugdunensis clinical isolates, mainly from orthopaedic infections but also from other clinical sources, were sub-grouped by ribotyping and dendrogram analysis.
31985269	8	5	dep	mass	1098:1101	arg1	R2					1104:1105	R2	1104:1105	R2 = 0.882	1104:1113	The content of exopolysaccharides was found to be to be strongly associated with the biofilm mass (R2 = 0.882), while the content of proteins turned out to be weakly (R2 = 0.465) and that of eDNA very weakly associated (R2 = 0.202) to the biofilm mass.
31985269	2	6	theme	complex	285:291	arg1	process					304:310	a complex pathogenic process	283:310	a complex pathogenic process that leads to well-established bacterial communities	283:363	Biofilm formation is a complex pathogenic process that leads to well-established bacterial communities.
31985269	2	6	theme	complex	285:291	arg1	formation					270:278	Biofilm formation	262:278	Biofilm formation	262:278	Biofilm formation is a complex pathogenic process that leads to well-established bacterial communities.
31985269	4	7	from	infections	539:548	arg1	isolates					505:512	twenty-two S. lugdunensis clinical isolates	470:512	twenty-two S. lugdunensis clinical isolates	470:512	Here, twenty-two S. lugdunensis clinical isolates, mainly from orthopaedic infections but also from other clinical sources, were sub-grouped by ribotyping and dendrogram analysis.
31985269	0	8	with	exopolysaccharides	78:95	arg1	mass					148:151	biofilm mass	140:151	biofilm mass	140:151	Various biofilm matrices of the emerging pathogen Staphylococcus lugdunensis: exopolysaccharides, proteins, eDNA and their correlation with biofilm mass.
31985269	4	9	dep	S.	481:482	arg1	lugdunensis					484:494	lugdunensis	484:494	lugdunensis	484:494	Here, twenty-two S. lugdunensis clinical isolates, mainly from orthopaedic infections but also from other clinical sources, were sub-grouped by ribotyping and dendrogram analysis.
31985269	3	10	from	composition	400:410	arg1	paucity					377:383	a paucity	375:383	a paucity of data on the composition of the biofilm matrix among S. lugdunensis strains	375:461	There is a paucity of data on the composition of the biofilm matrix among S. lugdunensis strains.
31985269	8	11	theme	biofilm	1090:1096	arg1	mass					1098:1101	the biofilm mass	1086:1101	the biofilm mass (R2 = 0.882)	1086:1114	The content of exopolysaccharides was found to be to be strongly associated with the biofilm mass (R2 = 0.882), while the content of proteins turned out to be weakly (R2 = 0.465) and that of eDNA very weakly associated (R2 = 0.202) to the biofilm mass.
31985269	4	12	theme	dendrogram	623:632	arg1	analysis					634:641	ribotyping and dendrogram analysis	608:641	analysis	634:641	Here, twenty-two S. lugdunensis clinical isolates, mainly from orthopaedic infections but also from other clinical sources, were sub-grouped by ribotyping and dendrogram analysis.
31985269	8	13	dep	weakly	1164:1169	arg1	R2					1172:1173	R2	1172:1173	R2 = 0.465	1172:1181	The content of exopolysaccharides was found to be to be strongly associated with the biofilm mass (R2 = 0.882), while the content of proteins turned out to be weakly (R2 = 0.465) and that of eDNA very weakly associated (R2 = 0.202) to the biofilm mass.
31985269	8	13	dep	weakly	1164:1169	arg1	weakly					1164:1169	weakly	1164:1169	weakly	1164:1169	The content of exopolysaccharides was found to be to be strongly associated with the biofilm mass (R2 = 0.882), while the content of proteins turned out to be weakly (R2 = 0.465) and that of eDNA very weakly associated (R2 = 0.202) to the biofilm mass.
31985269	8	13	dep	weakly	1164:1169	arg1	content					1127:1133	the content	1123:1133	the content of proteins	1123:1145	The content of exopolysaccharides was found to be to be strongly associated with the biofilm mass (R2 = 0.882), while the content of proteins turned out to be weakly (R2 = 0.465) and that of eDNA very weakly associated (R2 = 0.202) to the biofilm mass.
31985269	6	14	theme	Biofilm	838:844	arg1	morphology					846:855	Biofilm morphology	838:855	Biofilm morphology	838:855	Biofilm morphology was investigated under confocal laser scanning microscopy (CLSM).
31985269	8	15	dep	=	1175:1175	arg1	0.465					1177:1181	0.465	1177:1181	0.465	1177:1181	The content of exopolysaccharides was found to be to be strongly associated with the biofilm mass (R2 = 0.882), while the content of proteins turned out to be weakly (R2 = 0.465) and that of eDNA very weakly associated (R2 = 0.202) to the biofilm mass.
31985269	3	16	theme	data	388:391	arg1	paucity					377:383	a paucity	375:383	a paucity of data on the composition of the biofilm matrix among S. lugdunensis strains	375:461	There is a paucity of data on the composition of the biofilm matrix among S. lugdunensis strains.
31985269	3	17	theme	S.	440:441	arg1	strains					455:461	S. lugdunensis strains	440:461	S. lugdunensis strains	440:461	There is a paucity of data on the composition of the biofilm matrix among S. lugdunensis strains.
31985269	3	18	theme	biofilm	419:425	arg1	matrix					427:432	the biofilm matrix	415:432	the biofilm matrix	415:432	There is a paucity of data on the composition of the biofilm matrix among S. lugdunensis strains.
31985269	0	19	theme	biofilm	8:14	arg1	matrices					16:23	Various biofilm matrices	0:23	Various biofilm matrices of the emerging pathogen Staphylococcus lugdunensis: exopolysaccharides, proteins, eDNA and their correlation with biofilm mass.	0:152	Various biofilm matrices of the emerging pathogen Staphylococcus lugdunensis: exopolysaccharides, proteins, eDNA and their correlation with biofilm mass.
31985269	0	20	with	eDNA	108:111	arg1	mass					148:151	biofilm mass	140:151	biofilm mass	140:151	Various biofilm matrices of the emerging pathogen Staphylococcus lugdunensis: exopolysaccharides, proteins, eDNA and their correlation with biofilm mass.
31985269	1	21	theme	emerging	187:194	arg1	pathogen					210:217	an emerging high-virulent pathogen	184:217	an emerging high-virulent pathogen causative of hospital-acquired infections	184:259	Staphylococcus lugdunensis is an emerging high-virulent pathogen causative of hospital-acquired infections.
31985269	1	21	theme	emerging	187:194	arg1	lugdunensis					169:179	Staphylococcus lugdunensis	154:179	Staphylococcus lugdunensis	154:179	Staphylococcus lugdunensis is an emerging high-virulent pathogen causative of hospital-acquired infections.
31985269	0	22	theme	Various	0:6	arg1	matrices					16:23	Various biofilm matrices	0:23	Various biofilm matrices of the emerging pathogen Staphylococcus lugdunensis: exopolysaccharides, proteins, eDNA and their correlation with biofilm mass.	0:152	Various biofilm matrices of the emerging pathogen Staphylococcus lugdunensis: exopolysaccharides, proteins, eDNA and their correlation with biofilm mass.
31985269	8	23	theme	=	1107:1107	arg1	R2					1104:1105	R2	1104:1105	R2 = 0.882	1104:1113	The content of exopolysaccharides was found to be to be strongly associated with the biofilm mass (R2 = 0.882), while the content of proteins turned out to be weakly (R2 = 0.465) and that of eDNA very weakly associated (R2 = 0.202) to the biofilm mass.
31985269	7	24	theme	matrix	985:990	arg1	composition					992:1002	matrix composition	985:1002	matrix composition	985:1002	Isolates displayed intriguing diversities in biofilm mass and matrix composition.
31985269	1	25	theme	high-virulent	196:208	arg1	pathogen					210:217	an emerging high-virulent pathogen	184:217	an emerging high-virulent pathogen causative of hospital-acquired infections	184:259	Staphylococcus lugdunensis is an emerging high-virulent pathogen causative of hospital-acquired infections.
31985269	1	25	theme	high-virulent	196:208	arg1	lugdunensis					169:179	Staphylococcus lugdunensis	154:179	Staphylococcus lugdunensis	154:179	Staphylococcus lugdunensis is an emerging high-virulent pathogen causative of hospital-acquired infections.
31985269	8	26	theme	=	1175:1175	arg1	R2					1172:1173	R2	1172:1173	R2 = 0.465	1172:1181	The content of exopolysaccharides was found to be to be strongly associated with the biofilm mass (R2 = 0.882), while the content of proteins turned out to be weakly (R2 = 0.465) and that of eDNA very weakly associated (R2 = 0.202) to the biofilm mass.
31985269	8	26	theme	=	1175:1175	arg1	weakly					1164:1169	weakly	1164:1169	weakly	1164:1169	The content of exopolysaccharides was found to be to be strongly associated with the biofilm mass (R2 = 0.882), while the content of proteins turned out to be weakly (R2 = 0.465) and that of eDNA very weakly associated (R2 = 0.202) to the biofilm mass.
31985269	8	26	theme	=	1175:1175	arg1	content					1127:1133	the content	1123:1133	the content of proteins	1123:1145	The content of exopolysaccharides was found to be to be strongly associated with the biofilm mass (R2 = 0.882), while the content of proteins turned out to be weakly (R2 = 0.465) and that of eDNA very weakly associated (R2 = 0.202) to the biofilm mass.
31985269	5	27	theme	TOTO-1	743:748	arg1	dyes					750:753	TOTO-1 dyes	743:753	TOTO-1 dyes	743:753	Biofilms were analysed by fluorimetric methods based on FITC-Wheat Germ Agglutinin, SYPRO Ruby and TOTO-1 dyes to detect exopolysaccharides, proteins and extracellular DNA (eDNA), respectively.
31985269	0	28	with	proteins	98:105	arg1	mass					148:151	biofilm mass	140:151	biofilm mass	140:151	Various biofilm matrices of the emerging pathogen Staphylococcus lugdunensis: exopolysaccharides, proteins, eDNA and their correlation with biofilm mass.
31985269	2	29	theme	bacterial	343:351	arg1	communities					353:363	well-established bacterial communities	326:363	well-established bacterial communities	326:363	Biofilm formation is a complex pathogenic process that leads to well-established bacterial communities.
31985269	8	30	dep	=	1228:1228	arg1	0.202					1230:1234	0.202	1230:1234	0.202	1230:1234	The content of exopolysaccharides was found to be to be strongly associated with the biofilm mass (R2 = 0.882), while the content of proteins turned out to be weakly (R2 = 0.465) and that of eDNA very weakly associated (R2 = 0.202) to the biofilm mass.
31985269	4	31	theme	S.	481:482	arg1	isolates					505:512	twenty-two S. lugdunensis clinical isolates	470:512	twenty-two S. lugdunensis clinical isolates	470:512	Here, twenty-two S. lugdunensis clinical isolates, mainly from orthopaedic infections but also from other clinical sources, were sub-grouped by ribotyping and dendrogram analysis.
31985269	2	32	theme	well-established	326:341	arg1	communities					353:363	well-established bacterial communities	326:363	well-established bacterial communities	326:363	Biofilm formation is a complex pathogenic process that leads to well-established bacterial communities.
31985269	1	33	theme	causative	219:227	arg1	pathogen					210:217	an emerging high-virulent pathogen	184:217	an emerging high-virulent pathogen causative of hospital-acquired infections	184:259	Staphylococcus lugdunensis is an emerging high-virulent pathogen causative of hospital-acquired infections.
31985269	1	33	theme	causative	219:227	arg1	lugdunensis					169:179	Staphylococcus lugdunensis	154:179	Staphylococcus lugdunensis	154:179	Staphylococcus lugdunensis is an emerging high-virulent pathogen causative of hospital-acquired infections.
31985269	0	34	theme	emerging	32:39	arg1	pathogen					41:48	the emerging pathogen	28:48	the emerging pathogen	28:48	Various biofilm matrices of the emerging pathogen Staphylococcus lugdunensis: exopolysaccharides, proteins, eDNA and their correlation with biofilm mass.
31985269	8	35	dep	associated	1213:1222	arg1	R2					1225:1226	R2	1225:1226	R2 = 0.202	1225:1234	The content of exopolysaccharides was found to be to be strongly associated with the biofilm mass (R2 = 0.882), while the content of proteins turned out to be weakly (R2 = 0.465) and that of eDNA very weakly associated (R2 = 0.202) to the biofilm mass.
31985269	0	36	theme	biofilm	140:146	arg1	mass					148:151	biofilm mass	140:151	biofilm mass	140:151	Various biofilm matrices of the emerging pathogen Staphylococcus lugdunensis: exopolysaccharides, proteins, eDNA and their correlation with biofilm mass.
31985269	3	37	from	data	388:391	arg1	composition					400:410	the composition	396:410	the composition of the biofilm matrix among S. lugdunensis strains	396:461	There is a paucity of data on the composition of the biofilm matrix among S. lugdunensis strains.
31985269	8	38	theme	=	1228:1228	arg1	R2					1225:1226	R2	1225:1226	R2 = 0.202	1225:1234	The content of exopolysaccharides was found to be to be strongly associated with the biofilm mass (R2 = 0.882), while the content of proteins turned out to be weakly (R2 = 0.465) and that of eDNA very weakly associated (R2 = 0.202) to the biofilm mass.
31985269	8	39	theme	biofilm	1244:1250	arg1	mass					1252:1255	the biofilm mass	1240:1255	the biofilm mass	1240:1255	The content of exopolysaccharides was found to be to be strongly associated with the biofilm mass (R2 = 0.882), while the content of proteins turned out to be weakly (R2 = 0.465) and that of eDNA very weakly associated (R2 = 0.202) to the biofilm mass.
31985269	3	40	theme	lugdunensis	443:453	arg1	strains					455:461	S. lugdunensis strains	440:461	S. lugdunensis strains	440:461	There is a paucity of data on the composition of the biofilm matrix among S. lugdunensis strains.
31985269	4	41	theme	other	564:568	arg1	sources					579:585	other clinical sources	564:585	other clinical sources	564:585	Here, twenty-two S. lugdunensis clinical isolates, mainly from orthopaedic infections but also from other clinical sources, were sub-grouped by ribotyping and dendrogram analysis.
31985269	4	42	theme	clinical	496:503	arg1	isolates					505:512	twenty-two S. lugdunensis clinical isolates	470:512	twenty-two S. lugdunensis clinical isolates	470:512	Here, twenty-two S. lugdunensis clinical isolates, mainly from orthopaedic infections but also from other clinical sources, were sub-grouped by ribotyping and dendrogram analysis.
31985269	2	43	theme	Biofilm	262:268	arg1	process					304:310	a complex pathogenic process	283:310	a complex pathogenic process that leads to well-established bacterial communities	283:363	Biofilm formation is a complex pathogenic process that leads to well-established bacterial communities.
31985269	2	43	theme	Biofilm	262:268	arg1	formation					270:278	Biofilm formation	262:278	Biofilm formation	262:278	Biofilm formation is a complex pathogenic process that leads to well-established bacterial communities.
31985269	1	44	theme	hospital-acquired	232:248	arg1	infections					250:259	hospital-acquired infections	232:259	hospital-acquired infections	232:259	Staphylococcus lugdunensis is an emerging high-virulent pathogen causative of hospital-acquired infections.
31985269	3	45	theme	matrix	427:432	arg1	composition					400:410	the composition	396:410	the composition of the biofilm matrix among S. lugdunensis strains	396:461	There is a paucity of data on the composition of the biofilm matrix among S. lugdunensis strains.
31985269	0	46	theme	pathogen	41:48	arg1	matrices					16:23	Various biofilm matrices	0:23	Various biofilm matrices of the emerging pathogen Staphylococcus lugdunensis: exopolysaccharides, proteins, eDNA and their correlation with biofilm mass.	0:152	Various biofilm matrices of the emerging pathogen Staphylococcus lugdunensis: exopolysaccharides, proteins, eDNA and their correlation with biofilm mass.
31985269	5	47	theme	SYPRO	728:732	arg1	Ruby					734:737	SYPRO Ruby	728:737	SYPRO Ruby	728:737	Biofilms were analysed by fluorimetric methods based on FITC-Wheat Germ Agglutinin, SYPRO Ruby and TOTO-1 dyes to detect exopolysaccharides, proteins and extracellular DNA (eDNA), respectively.
31985269	6	48	theme	scanning	895:902	arg1	CLSM					916:919	CLSM	916:919	CLSM	916:919	Biofilm morphology was investigated under confocal laser scanning microscopy (CLSM).
31985269	6	48	theme	scanning	895:902	arg1	microscopy					904:913	confocal laser scanning microscopy	880:913	confocal laser scanning microscopy (CLSM)	880:920	Biofilm morphology was investigated under confocal laser scanning microscopy (CLSM).
31985269	5	49	theme	fluorimetric	670:681	arg1	methods					683:689	fluorimetric methods	670:689	fluorimetric methods based on FITC-Wheat Germ Agglutinin, SYPRO Ruby and TOTO-1 dyes to detect exopolysaccharides, proteins and extracellular DNA (eDNA), respectively	670:835	Biofilms were analysed by fluorimetric methods based on FITC-Wheat Germ Agglutinin, SYPRO Ruby and TOTO-1 dyes to detect exopolysaccharides, proteins and extracellular DNA (eDNA), respectively.
31985269	5	50	theme	Germ	711:714	arg1	Agglutinin					716:725	FITC-Wheat Germ Agglutinin	700:725	FITC-Wheat Germ Agglutinin	700:725	Biofilms were analysed by fluorimetric methods based on FITC-Wheat Germ Agglutinin, SYPRO Ruby and TOTO-1 dyes to detect exopolysaccharides, proteins and extracellular DNA (eDNA), respectively.
31985269	7	51	theme	biofilm	968:974	arg1	mass					976:979	biofilm mass	968:979	biofilm mass	968:979	Isolates displayed intriguing diversities in biofilm mass and matrix composition.
31985269	4	52	theme	clinical	570:577	arg1	sources					579:585	other clinical sources	564:585	other clinical sources	564:585	Here, twenty-two S. lugdunensis clinical isolates, mainly from orthopaedic infections but also from other clinical sources, were sub-grouped by ribotyping and dendrogram analysis.
31985269	6	53	theme	laser	889:893	arg1	CLSM					916:919	CLSM	916:919	CLSM	916:919	Biofilm morphology was investigated under confocal laser scanning microscopy (CLSM).
31985269	6	53	theme	laser	889:893	arg1	microscopy					904:913	confocal laser scanning microscopy	880:913	confocal laser scanning microscopy (CLSM)	880:920	Biofilm morphology was investigated under confocal laser scanning microscopy (CLSM).
31985269	2	54	theme	pathogenic	293:302	arg1	process					304:310	a complex pathogenic process	283:310	a complex pathogenic process that leads to well-established bacterial communities	283:363	Biofilm formation is a complex pathogenic process that leads to well-established bacterial communities.
31985269	2	54	theme	pathogenic	293:302	arg1	formation					270:278	Biofilm formation	262:278	Biofilm formation	262:278	Biofilm formation is a complex pathogenic process that leads to well-established bacterial communities.
31985269	1	55	theme	infections	250:259	arg1	causative					219:227	causative	219:227	causative	219:227	Staphylococcus lugdunensis is an emerging high-virulent pathogen causative of hospital-acquired infections.
31985269	8	56	theme	proteins	1138:1145	arg1	R2					1172:1173	R2	1172:1173	R2 = 0.465	1172:1181	The content of exopolysaccharides was found to be to be strongly associated with the biofilm mass (R2 = 0.882), while the content of proteins turned out to be weakly (R2 = 0.465) and that of eDNA very weakly associated (R2 = 0.202) to the biofilm mass.
31985269	8	56	theme	proteins	1138:1145	arg1	weakly					1164:1169	weakly	1164:1169	weakly	1164:1169	The content of exopolysaccharides was found to be to be strongly associated with the biofilm mass (R2 = 0.882), while the content of proteins turned out to be weakly (R2 = 0.465) and that of eDNA very weakly associated (R2 = 0.202) to the biofilm mass.
31985269	8	56	theme	proteins	1138:1145	arg1	content					1127:1133	the content	1123:1133	the content of proteins	1123:1145	The content of exopolysaccharides was found to be to be strongly associated with the biofilm mass (R2 = 0.882), while the content of proteins turned out to be weakly (R2 = 0.465) and that of eDNA very weakly associated (R2 = 0.202) to the biofilm mass.
31985269	6	57	theme	confocal	880:887	arg1	CLSM					916:919	CLSM	916:919	CLSM	916:919	Biofilm morphology was investigated under confocal laser scanning microscopy (CLSM).
31985269	6	57	theme	confocal	880:887	arg1	microscopy					904:913	confocal laser scanning microscopy	880:913	confocal laser scanning microscopy (CLSM)	880:920	Biofilm morphology was investigated under confocal laser scanning microscopy (CLSM).
31985269	0	58	dep	matrices	16:23	arg1	exopolysaccharides					78:95	exopolysaccharides	78:95	exopolysaccharides	78:95	Various biofilm matrices of the emerging pathogen Staphylococcus lugdunensis: exopolysaccharides, proteins, eDNA and their correlation with biofilm mass.
31985269	0	58	dep	matrices	16:23	arg1	eDNA					108:111	eDNA	108:111	eDNA	108:111	Various biofilm matrices of the emerging pathogen Staphylococcus lugdunensis: exopolysaccharides, proteins, eDNA and their correlation with biofilm mass.
31985269	0	58	dep	matrices	16:23	arg1	correlation					123:133	their correlation	117:133	their correlation with biofilm mass	117:151	Various biofilm matrices of the emerging pathogen Staphylococcus lugdunensis: exopolysaccharides, proteins, eDNA and their correlation with biofilm mass.
31985269	0	58	dep	matrices	16:23	arg1	proteins					98:105	proteins	98:105	proteins	98:105	Various biofilm matrices of the emerging pathogen Staphylococcus lugdunensis: exopolysaccharides, proteins, eDNA and their correlation with biofilm mass.
31985269	0	59	with	correlation	123:133	arg1	mass					148:151	biofilm mass	140:151	biofilm mass	140:151	Various biofilm matrices of the emerging pathogen Staphylococcus lugdunensis: exopolysaccharides, proteins, eDNA and their correlation with biofilm mass.
31985269	5	60	theme	extracellular	798:810	arg1	DNA					812:814	extracellular DNA	798:814	extracellular DNA (eDNA)	798:821	Biofilms were analysed by fluorimetric methods based on FITC-Wheat Germ Agglutinin, SYPRO Ruby and TOTO-1 dyes to detect exopolysaccharides, proteins and extracellular DNA (eDNA), respectively.
31985269	5	60	theme	extracellular	798:810	arg1	eDNA					817:820	eDNA	817:820	eDNA	817:820	Biofilms were analysed by fluorimetric methods based on FITC-Wheat Germ Agglutinin, SYPRO Ruby and TOTO-1 dyes to detect exopolysaccharides, proteins and extracellular DNA (eDNA), respectively.
31985269	3	61	from	paucity	377:383	arg1	composition					400:410	the composition	396:410	the composition of the biofilm matrix among S. lugdunensis strains	396:461	There is a paucity of data on the composition of the biofilm matrix among S. lugdunensis strains.
31985269	8	62	dep	=	1107:1107	arg1	0.882					1109:1113	0.882	1109:1113	0.882	1109:1113	The content of exopolysaccharides was found to be to be strongly associated with the biofilm mass (R2 = 0.882), while the content of proteins turned out to be weakly (R2 = 0.465) and that of eDNA very weakly associated (R2 = 0.202) to the biofilm mass.
31985269	8	63	theme	exopolysaccharides	1020:1037	arg1	content					1009:1015	The content	1005:1015	The content of exopolysaccharides	1005:1037	The content of exopolysaccharides was found to be to be strongly associated with the biofilm mass (R2 = 0.882), while the content of proteins turned out to be weakly (R2 = 0.465) and that of eDNA very weakly associated (R2 = 0.202) to the biofilm mass.
32871466	2	0	theme	composite	556:564	arg1	beads					566:570	composite beads	556:570	composite beads	556:570	Physiochemical characteristics of composite beads were analyzed using FTIR, SEM, TGA, and BET.
32871466	1	1	theme	synthesized	349:359	arg1	materials					361:369	synthesized materials	349:369	synthesized materials	349:369	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	1	2	theme	carbonized	256:265	arg1	material					267:274	carbonized material	256:274	carbonized material	256:274	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	7	3	with	agreement	1322:1330	arg1	results					1351:1357	investigation results	1337:1357	investigation results	1337:1357	The fixed-bed column data were evaluated using several kinetic models and among them, Thomas model showed the best agreement with investigation results.
32871466	0	4	dep	beads	140:144	arg1	Batch					147:151	Batch	147:151	Batch	147:151	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	4	dep	beads	140:144	arg1	beads					140:144	activated sugarcane baggsse charcoal beads	103:144	activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study	103:238	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	4	dep	beads	140:144	arg1	study					234:238	continuous fixed bed column study	206:238	continuous fixed bed column study	206:238	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	4	dep	beads	140:144	arg1	optimization					154:165	optimization	154:165	optimization using response surface methodology	154:200	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	1	5	used	used	448:451	arg2	beads					423:427	Sodium Alginate/SBAC composite beads	392:427	Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water	392:519	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	1	5	used	used	448:451	arg2	adsorbent					459:467	adsorbent	459:467	adsorbent	459:467	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	6	6	theme	response	976:983	arg1	methodology					993:1003	response surface methodology	976:1003	response surface methodology	976:1003	By using response surface methodology, the optimum conditions for MG adsorption on composite beads were found to be 115.43 min, 0.3 g/L and pH 8 for contact time, adsorbent mass, and pH respectively and MG adsorption efficiency was 97.88%.
32871466	1	7	attach	present	504:510	arg2	green					489:493	malachite green	479:493	malachite green dye (MG) present in water	479:519	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	1	7	attach	present	504:510	arg1	water					515:519	water	515:519	water	515:519	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	0	8	theme	fixed	217:221	arg1	beads					140:144	activated sugarcane baggsse charcoal beads	103:144	activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study	103:238	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	8	theme	fixed	217:221	arg1	study					234:238	continuous fixed bed column study	206:238	continuous fixed bed column study	206:238	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	6	9	theme	adsorption	1173:1182	arg1	efficiency					1184:1193	MG adsorption efficiency	1170:1193	MG adsorption efficiency	1170:1193	By using response surface methodology, the optimum conditions for MG adsorption on composite beads were found to be 115.43 min, 0.3 g/L and pH 8 for contact time, adsorbent mass, and pH respectively and MG adsorption efficiency was 97.88%.
32871466	7	10	theme	kinetic	1262:1268	arg1	models					1270:1275	several kinetic models	1254:1275	several kinetic models	1254:1275	The fixed-bed column data were evaluated using several kinetic models and among them, Thomas model showed the best agreement with investigation results.
32871466	0	11	theme	activated	103:111	arg1	Batch					147:151	Batch	147:151	Batch	147:151	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	11	theme	activated	103:111	arg1	beads					140:144	activated sugarcane baggsse charcoal beads	103:144	activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study	103:238	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	11	theme	activated	103:111	arg1	study					234:238	continuous fixed bed column study	206:238	continuous fixed bed column study	206:238	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	11	theme	activated	103:111	arg1	optimization					154:165	optimization	154:165	optimization using response surface methodology	154:200	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	12	theme	sugarcane	113:121	arg1	Batch					147:151	Batch	147:151	Batch	147:151	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	12	theme	sugarcane	113:121	arg1	beads					140:144	activated sugarcane baggsse charcoal beads	103:144	activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study	103:238	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	12	theme	sugarcane	113:121	arg1	study					234:238	continuous fixed bed column study	206:238	continuous fixed bed column study	206:238	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	12	theme	sugarcane	113:121	arg1	optimization					154:165	optimization	154:165	optimization using response surface methodology	154:200	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	13	theme	continuous	206:215	arg1	beads					140:144	activated sugarcane baggsse charcoal beads	103:144	activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study	103:238	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	13	theme	continuous	206:215	arg1	study					234:238	continuous fixed bed column study	206:238	continuous fixed bed column study	206:238	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	5	14	theme	MG	952:953	arg1	adsorption					955:964	MG adsorption	952:964	MG adsorption	952:964	The thermodynamic study suggested the spontaneous and endothermic nature of MG adsorption.
32871466	0	15	theme	charcoal	131:138	arg1	Batch					147:151	Batch	147:151	Batch	147:151	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	15	theme	charcoal	131:138	arg1	beads					140:144	activated sugarcane baggsse charcoal beads	103:144	activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study	103:238	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	15	theme	charcoal	131:138	arg1	study					234:238	continuous fixed bed column study	206:238	continuous fixed bed column study	206:238	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	15	theme	charcoal	131:138	arg1	optimization					154:165	optimization	154:165	optimization using response surface methodology	154:200	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	16	theme	column	227:232	arg1	beads					140:144	activated sugarcane baggsse charcoal beads	103:144	activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study	103:238	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	16	theme	column	227:232	arg1	study					234:238	continuous fixed bed column study	206:238	continuous fixed bed column study	206:238	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	17	contain	containing	33:42	arg2	solution					44:51	solution	44:51	solution using bio-degradable Sodium alginate/NaOH	44:93	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	17	contain	containing	33:42	arg1	green					23:27	malachite green dye	13:31	malachite green dye containing solution using bio-degradable Sodium alginate/NaOH	13:93	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	5	18	theme	adsorption	955:964	arg1	nature					942:947	the spontaneous and endothermic nature	910:947	the spontaneous and endothermic nature of MG adsorption	910:964	The thermodynamic study suggested the spontaneous and endothermic nature of MG adsorption.
32871466	6	19	theme	MG	1033:1034	arg1	adsorption					1036:1045	MG adsorption	1033:1045	MG adsorption on composite beads	1033:1064	By using response surface methodology, the optimum conditions for MG adsorption on composite beads were found to be 115.43 min, 0.3 g/L and pH 8 for contact time, adsorbent mass, and pH respectively and MG adsorption efficiency was 97.88%.
32871466	0	20	theme	baggsse	123:129	arg1	Batch					147:151	Batch	147:151	Batch	147:151	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	20	theme	baggsse	123:129	arg1	beads					140:144	activated sugarcane baggsse charcoal beads	103:144	activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study	103:238	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	20	theme	baggsse	123:129	arg1	study					234:238	continuous fixed bed column study	206:238	continuous fixed bed column study	206:238	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	20	theme	baggsse	123:129	arg1	optimization					154:165	optimization	154:165	optimization using response surface methodology	154:200	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	21	theme	bed	223:225	arg1	beads					140:144	activated sugarcane baggsse charcoal beads	103:144	activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study	103:238	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	0	21	theme	bed	223:225	arg1	study					234:238	continuous fixed bed column study	206:238	continuous fixed bed column study	206:238	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	1	22	from	water	515:519	arg1	present					504:510	present	504:510	present	504:510	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	8	23	theme	dye	1502:1504	arg1	removal					1506:1512	dye removal	1502:1512	dye removal from wastewater	1502:1528	These results revealed that synthesized composite beads have a high affinity toward MG and it could be reasonable, eco-friendly adsorbent for dye removal from wastewater.
32871466	5	24	theme	spontaneous	914:924	arg1	nature					942:947	the spontaneous and endothermic nature	910:947	the spontaneous and endothermic nature of MG adsorption	910:964	The thermodynamic study suggested the spontaneous and endothermic nature of MG adsorption.
32871466	6	25	theme	composite	1050:1058	arg1	beads					1060:1064	composite beads	1050:1064	composite beads	1050:1064	By using response surface methodology, the optimum conditions for MG adsorption on composite beads were found to be 115.43 min, 0.3 g/L and pH 8 for contact time, adsorbent mass, and pH respectively and MG adsorption efficiency was 97.88%.
32871466	2	26	theme	Physiochemical	522:535	arg1	characteristics					537:551	Physiochemical characteristics	522:551	Physiochemical characteristics of composite beads	522:570	Physiochemical characteristics of composite beads were analyzed using FTIR, SEM, TGA, and BET.
32871466	3	27	theme	Langmuir	715:722	arg1	R2 = 0.925					731:740	R2 = 0.925	731:740	R2 = 0.925	731:740	Adsorption equilibrium data showed excellent fit to the Freundlich model (R2 = 0.994) than to the Langmuir model (R2 = 0.925).
32871466	3	27	theme	Langmuir	715:722	arg1	model					724:728	the Langmuir model	711:728	the Langmuir model (R2 = 0.925)	711:741	Adsorption equilibrium data showed excellent fit to the Freundlich model (R2 = 0.994) than to the Langmuir model (R2 = 0.925).
32871466	8	28	from	wastewater	1519:1528	arg1	removal					1506:1512	dye removal	1502:1512	dye removal from wastewater	1502:1528	These results revealed that synthesized composite beads have a high affinity toward MG and it could be reasonable, eco-friendly adsorbent for dye removal from wastewater.
32871466	6	29	from	adsorption	1036:1045	arg1	beads					1060:1064	composite beads	1050:1064	composite beads	1050:1064	By using response surface methodology, the optimum conditions for MG adsorption on composite beads were found to be 115.43 min, 0.3 g/L and pH 8 for contact time, adsorbent mass, and pH respectively and MG adsorption efficiency was 97.88%.
32871466	3	30	theme	excellent	652:660	arg1	fit					662:664	excellent fit	652:664	excellent fit to the Freundlich model (R2 = 0.994)	652:701	Adsorption equilibrium data showed excellent fit to the Freundlich model (R2 = 0.994) than to the Langmuir model (R2 = 0.925).
32871466	6	31	theme	surface	985:991	arg1	methodology					993:1003	response surface methodology	976:1003	response surface methodology	976:1003	By using response surface methodology, the optimum conditions for MG adsorption on composite beads were found to be 115.43 min, 0.3 g/L and pH 8 for contact time, adsorbent mass, and pH respectively and MG adsorption efficiency was 97.88%.
32871466	1	32	from	present	504:510	arg1	water					515:519	water	515:519	water	515:519	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	0	33	theme	green	23:27	arg1	Treatment					0:8	Treatment	0:8	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH	0:93	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	1	34	theme	sodium	295:300	arg1	SB					340:341	SB	340:341	SB	340:341	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	1	34	theme	sodium	295:300	arg1	bagasse					331:337	sodium hydroxide treated Sugar cane bagasse	295:337	sodium hydroxide treated Sugar cane bagasse (SB)	295:342	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	1	35	theme	malachite	479:487	arg1	green					489:493	malachite green	479:493	malachite green dye (MG) present in water	479:519	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	5	36	theme	endothermic	930:940	arg1	nature					942:947	the spontaneous and endothermic nature	910:947	the spontaneous and endothermic nature of MG adsorption	910:964	The thermodynamic study suggested the spontaneous and endothermic nature of MG adsorption.
32871466	7	37	theme	several	1254:1260	arg1	models					1270:1275	several kinetic models	1254:1275	several kinetic models	1254:1275	The fixed-bed column data were evaluated using several kinetic models and among them, Thomas model showed the best agreement with investigation results.
32871466	0	38	theme	malachite	13:21	arg1	green					23:27	malachite green dye	13:31	malachite green dye containing solution using bio-degradable Sodium alginate/NaOH	13:93	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	1	39	theme	hydroxide	302:310	arg1	SB					340:341	SB	340:341	SB	340:341	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	1	39	theme	hydroxide	302:310	arg1	bagasse					331:337	sodium hydroxide treated Sugar cane bagasse	295:337	sodium hydroxide treated Sugar cane bagasse (SB)	295:342	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	6	40	theme	adsorbent	1130:1138	arg1	mass					1140:1143	adsorbent mass	1130:1143	adsorbent mass	1130:1143	By using response surface methodology, the optimum conditions for MG adsorption on composite beads were found to be 115.43 min, 0.3 g/L and pH 8 for contact time, adsorbent mass, and pH respectively and MG adsorption efficiency was 97.88%.
32871466	3	41	theme	Adsorption	617:626	arg1	data					640:643	Adsorption equilibrium data	617:643	Adsorption equilibrium data	617:643	Adsorption equilibrium data showed excellent fit to the Freundlich model (R2 = 0.994) than to the Langmuir model (R2 = 0.925).
32871466	6	42	theme	contact	1116:1122	arg1	time					1124:1127	contact time	1116:1127	contact time	1116:1127	By using response surface methodology, the optimum conditions for MG adsorption on composite beads were found to be 115.43 min, 0.3 g/L and pH 8 for contact time, adsorbent mass, and pH respectively and MG adsorption efficiency was 97.88%.
32871466	1	43	theme	treated	312:318	arg1	SB					340:341	SB	340:341	SB	340:341	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	1	43	theme	treated	312:318	arg1	bagasse					331:337	sodium hydroxide treated Sugar cane bagasse	295:337	sodium hydroxide treated Sugar cane bagasse (SB)	295:342	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	1	44	dep	ware	371:374	arg1	used					376:379	used	376:379	ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water	371:519	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	3	45	theme	equilibrium	628:638	arg1	data					640:643	Adsorption equilibrium data	617:643	Adsorption equilibrium data	617:643	Adsorption equilibrium data showed excellent fit to the Freundlich model (R2 = 0.994) than to the Langmuir model (R2 = 0.925).
32871466	1	46	theme	Sugar	320:324	arg1	SB					340:341	SB	340:341	SB	340:341	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	1	46	theme	Sugar	320:324	arg1	bagasse					331:337	sodium hydroxide treated Sugar cane bagasse	295:337	sodium hydroxide treated Sugar cane bagasse (SB)	295:342	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	1	47	theme	Sodium	392:397	arg1	beads					423:427	Sodium Alginate/SBAC composite beads	392:427	Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water	392:519	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	1	47	theme	Sodium	392:397	arg1	adsorbent					459:467	adsorbent	459:467	adsorbent	459:467	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	7	48	theme	best	1317:1320	arg1	agreement					1322:1330	the best agreement	1313:1330	the best agreement with investigation results	1313:1357	The fixed-bed column data were evaluated using several kinetic models and among them, Thomas model showed the best agreement with investigation results.
32871466	1	49	theme	cane	326:329	arg1	SB					340:341	SB	340:341	SB	340:341	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	1	49	theme	cane	326:329	arg1	bagasse					331:337	sodium hydroxide treated Sugar cane bagasse	295:337	sodium hydroxide treated Sugar cane bagasse (SB)	295:342	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	1	50	theme	Alginate/SBAC	399:411	arg1	beads					423:427	Sodium Alginate/SBAC composite beads	392:427	Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water	392:519	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	1	50	theme	Alginate/SBAC	399:411	arg1	adsorbent					459:467	adsorbent	459:467	adsorbent	459:467	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	4	51	theme	removal	792:798	arg1	process					800:806	the MG removal process	785:806	the MG removal process	785:806	Adsorption kinetics study indicated that the MG removal process would be better described by the pseudo-second-order kinetic model.
32871466	2	52	theme	beads	566:570	arg1	characteristics					537:551	Physiochemical characteristics	522:551	Physiochemical characteristics of composite beads	522:570	Physiochemical characteristics of composite beads were analyzed using FTIR, SEM, TGA, and BET.
32871466	5	53	theme	thermodynamic	880:892	arg1	study					894:898	The thermodynamic study	876:898	The thermodynamic study	876:898	The thermodynamic study suggested the spontaneous and endothermic nature of MG adsorption.
32871466	4	54	theme	kinetic	861:867	arg1	model					869:873	the pseudo-second-order kinetic model	837:873	the pseudo-second-order kinetic model	837:873	Adsorption kinetics study indicated that the MG removal process would be better described by the pseudo-second-order kinetic model.
32871466	1	55	theme	composite	413:421	arg1	beads					423:427	Sodium Alginate/SBAC composite beads	392:427	Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water	392:519	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	1	55	theme	composite	413:421	arg1	adsorbent					459:467	adsorbent	459:467	adsorbent	459:467	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	4	56	theme	MG	789:790	arg1	process					800:806	the MG removal process	785:806	the MG removal process	785:806	Adsorption kinetics study indicated that the MG removal process would be better described by the pseudo-second-order kinetic model.
32871466	4	57	theme	pseudo-second-order	841:859	arg1	model					869:873	the pseudo-second-order kinetic model	837:873	the pseudo-second-order kinetic model	837:873	Adsorption kinetics study indicated that the MG removal process would be better described by the pseudo-second-order kinetic model.
32871466	6	58	theme	MG	1170:1171	arg1	efficiency					1184:1193	MG adsorption efficiency	1170:1193	MG adsorption efficiency	1170:1193	By using response surface methodology, the optimum conditions for MG adsorption on composite beads were found to be 115.43 min, 0.3 g/L and pH 8 for contact time, adsorbent mass, and pH respectively and MG adsorption efficiency was 97.88%.
32871466	0	59	theme	surface	182:188	arg1	methodology					190:200	response surface methodology	173:200	response surface methodology	173:200	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	8	60	theme	high	1423:1426	arg1	affinity					1428:1435	a high affinity	1421:1435	a high affinity toward MG	1421:1445	These results revealed that synthesized composite beads have a high affinity toward MG and it could be reasonable, eco-friendly adsorbent for dye removal from wastewater.
32871466	4	61	theme	kinetics	755:762	arg1	study					764:768	Adsorption kinetics study	744:768	Adsorption kinetics study	744:768	Adsorption kinetics study indicated that the MG removal process would be better described by the pseudo-second-order kinetic model.
32871466	7	62	theme	fixed-bed	1211:1219	arg1	data					1228:1231	The fixed-bed column data	1207:1231	The fixed-bed column data	1207:1231	The fixed-bed column data were evaluated using several kinetic models and among them, Thomas model showed the best agreement with investigation results.
32871466	7	63	theme	investigation	1337:1349	arg1	results					1351:1357	investigation results	1337:1357	investigation results	1337:1357	The fixed-bed column data were evaluated using several kinetic models and among them, Thomas model showed the best agreement with investigation results.
32871466	6	64	theme	optimum	1010:1016	arg1	conditions					1018:1027	the optimum conditions	1006:1027	the optimum conditions for MG adsorption on composite beads	1006:1064	By using response surface methodology, the optimum conditions for MG adsorption on composite beads were found to be 115.43 min, 0.3 g/L and pH 8 for contact time, adsorbent mass, and pH respectively and MG adsorption efficiency was 97.88%.
32871466	6	64	theme	optimum	1010:1016	arg1	115.43 min					1083:1092	115.43 min	1083:1092	115.43 min	1083:1092	By using response surface methodology, the optimum conditions for MG adsorption on composite beads were found to be 115.43 min, 0.3 g/L and pH 8 for contact time, adsorbent mass, and pH respectively and MG adsorption efficiency was 97.88%.
32871466	0	65	theme	response	173:180	arg1	methodology					190:200	response surface methodology	173:200	response surface methodology	173:200	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	4	66	theme	Adsorption	744:753	arg1	kinetics					755:762	Adsorption kinetics	744:762	Adsorption kinetics study	744:768	Adsorption kinetics study indicated that the MG removal process would be better described by the pseudo-second-order kinetic model.
32871466	7	67	theme	column	1221:1226	arg1	data					1228:1231	The fixed-bed column data	1207:1231	The fixed-bed column data	1207:1231	The fixed-bed column data were evaluated using several kinetic models and among them, Thomas model showed the best agreement with investigation results.
32871466	8	68	theme	composite	1400:1408	arg1	beads					1410:1414	synthesized composite beads	1388:1414	synthesized composite beads	1388:1414	These results revealed that synthesized composite beads have a high affinity toward MG and it could be reasonable, eco-friendly adsorbent for dye removal from wastewater.
32871466	0	69	theme	Sodium	74:79	arg1	alginate/NaOH					81:93	bio-degradable Sodium alginate/NaOH	59:93	bio-degradable Sodium alginate/NaOH	59:93	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	8	70	theme	synthesized	1388:1398	arg1	beads					1410:1414	synthesized composite beads	1388:1414	synthesized composite beads	1388:1414	These results revealed that synthesized composite beads have a high affinity toward MG and it could be reasonable, eco-friendly adsorbent for dye removal from wastewater.
32871466	1	71	theme	present	504:510	arg1	green					489:493	malachite green	479:493	malachite green dye (MG) present in water	479:519	In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water.
32871466	0	72	theme	bio-degradable	59:72	arg1	alginate/NaOH					81:93	bio-degradable Sodium alginate/NaOH	59:93	bio-degradable Sodium alginate/NaOH	59:93	Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study.
32871466	3	73	theme	Freundlich	673:682	arg1	R2 = 0.994					691:700	R2 = 0.994	691:700	R2 = 0.994	691:700	Adsorption equilibrium data showed excellent fit to the Freundlich model (R2 = 0.994) than to the Langmuir model (R2 = 0.925).
32871466	3	73	theme	Freundlich	673:682	arg1	model					684:688	the Freundlich model	669:688	the Freundlich model (R2 = 0.994)	669:701	Adsorption equilibrium data showed excellent fit to the Freundlich model (R2 = 0.994) than to the Langmuir model (R2 = 0.925).
32871466	7	74	theme	Thomas	1293:1298	arg1	model					1300:1304	Thomas model	1293:1304	Thomas model	1293:1304	The fixed-bed column data were evaluated using several kinetic models and among them, Thomas model showed the best agreement with investigation results.
32871466	8	75	contain	have	1416:1419	arg2	affinity					1428:1435	a high affinity	1421:1435	a high affinity toward MG	1421:1445	These results revealed that synthesized composite beads have a high affinity toward MG and it could be reasonable, eco-friendly adsorbent for dye removal from wastewater.
32871466	8	75	contain	have	1416:1419	arg1	beads					1410:1414	synthesized composite beads	1388:1414	synthesized composite beads	1388:1414	These results revealed that synthesized composite beads have a high affinity toward MG and it could be reasonable, eco-friendly adsorbent for dye removal from wastewater.
32871466	8	76	dep	reasonable	1463:1472	arg1	adsorbent					1488:1496	adsorbent	1488:1496	adsorbent	1488:1496	These results revealed that synthesized composite beads have a high affinity toward MG and it could be reasonable, eco-friendly adsorbent for dye removal from wastewater.
33800956	1	0	theme	variable	326:333	arg1	properties					352:361	variable glass transition properties	326:361	variable glass transition properties	326:361	Crosslinking of hydroxypropyl methyl cellulose (HPMC) and acrylic acid (AAc) was carried out at various compositions to develop a high-solid matrix with variable glass transition properties.
33800956	4	1	theme	small-deformation	858:874	arg1	in-shear					896:903	small-deformation dynamic oscillation in-shear	858:903	small-deformation dynamic oscillation in-shear	858:903	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	4	2	theme	network	702:708	arg1	properties					684:693	Structural properties	673:693	Structural properties of the network	673:708	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	7	3	theme	compound	1478:1485	arg1	delivery					1487:1494	targeted bioactive compound delivery	1459:1494	targeted bioactive compound delivery	1459:1494	Complementary TGA work demonstrates that the initial composition of all the HPMC-AAc networks is maintained up to 200 °C, an outcome that bodes well for applications of targeted bioactive compound delivery.
33800956	1	4	theme	glass	335:339	arg1	properties					352:361	variable glass transition properties	326:361	variable glass transition properties	326:361	Crosslinking of hydroxypropyl methyl cellulose (HPMC) and acrylic acid (AAc) was carried out at various compositions to develop a high-solid matrix with variable glass transition properties.
33800956	6	5	theme	rubbery	1273:1279	arg1	plateau					1281:1287	an extensive rubbery plateau	1260:1287	an extensive rubbery plateau	1260:1287	There is a considerable manipulation of the rheological and calorimetric glass transition temperatures as a function of the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau.
33800956	4	6	theme	electron	952:959	arg1	microscopy					961:970	scanning electron microscopy	943:970	scanning electron microscopy (SEM)	943:976	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	4	6	theme	electron	952:959	arg1	SEM					973:975	SEM	973:975	SEM	973:975	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	1	7	theme	transition	341:350	arg1	properties					352:361	variable glass transition properties	326:361	variable glass transition properties	326:361	Crosslinking of hydroxypropyl methyl cellulose (HPMC) and acrylic acid (AAc) was carried out at various compositions to develop a high-solid matrix with variable glass transition properties.
33800956	4	8	theme	oscillation	884:894	arg1	in-shear					896:903	small-deformation dynamic oscillation in-shear	858:903	small-deformation dynamic oscillation in-shear	858:903	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	1	9	with	matrix	314:319	arg1	properties					352:361	variable glass transition properties	326:361	variable glass transition properties	326:361	Crosslinking of hydroxypropyl methyl cellulose (HPMC) and acrylic acid (AAc) was carried out at various compositions to develop a high-solid matrix with variable glass transition properties.
33800956	0	10	theme	Acrylic	79:85	arg1	Acid-N					87:92	Acrylic Acid-N	79:92	Acrylic Acid-N	79:92	Manipulation of the Glass Transition Properties of a High-Solid System Made of Acrylic Acid-N,N'-Methylenebisacrylamide Copolymer Grafted on Hydroxypropyl Methyl Cellulose.
33800956	4	11	theme	dynamic	876:882	arg1	in-shear					896:903	small-deformation dynamic oscillation in-shear	858:903	small-deformation dynamic oscillation in-shear	858:903	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	6	12	theme	considerable	1083:1094	arg1	manipulation					1096:1107	a considerable manipulation	1081:1107	a considerable manipulation of the rheological and calorimetric glass transition temperatures as a function of the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau	1081:1287	There is a considerable manipulation of the rheological and calorimetric glass transition temperatures as a function of the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau.
33800956	0	13	theme	N'-Methylenebisacrylamide	94:118	arg1	Copolymer					120:128	N'-Methylenebisacrylamide Copolymer	94:128	N'-Methylenebisacrylamide Copolymer	94:128	Manipulation of the Glass Transition Properties of a High-Solid System Made of Acrylic Acid-N,N'-Methylenebisacrylamide Copolymer Grafted on Hydroxypropyl Methyl Cellulose.
33800956	0	13	theme	N'-Methylenebisacrylamide	94:118	arg1	System					64:69	a High-Solid System	51:69	a High-Solid System Made of Acrylic Acid-N	51:92	Manipulation of the Glass Transition Properties of a High-Solid System Made of Acrylic Acid-N,N'-Methylenebisacrylamide Copolymer Grafted on Hydroxypropyl Methyl Cellulose.
33800956	7	14	theme	HPMC-AAc	1366:1373	arg1	networks					1375:1382	all the HPMC-AAc networks	1358:1382	all the HPMC-AAc networks	1358:1382	Complementary TGA work demonstrates that the initial composition of all the HPMC-AAc networks is maintained up to 200 °C, an outcome that bodes well for applications of targeted bioactive compound delivery.
33800956	4	15	theme	X-ray	781:785	arg1	XRD					800:802	XRD	800:802	XRD	800:802	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	4	15	theme	X-ray	781:785	arg1	diffraction					787:797	X-ray diffraction	781:797	X-ray diffraction (XRD)	781:803	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	4	16	theme	Structural	673:682	arg1	properties					684:693	Structural properties	673:693	Structural properties of the network	673:708	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	1	17	theme	hydroxypropyl	189:201	arg1	HPMC					221:224	HPMC	221:224	HPMC	221:224	Crosslinking of hydroxypropyl methyl cellulose (HPMC) and acrylic acid (AAc) was carried out at various compositions to develop a high-solid matrix with variable glass transition properties.
33800956	1	17	theme	hydroxypropyl	189:201	arg1	cellulose					210:218	hydroxypropyl methyl cellulose	189:218	hydroxypropyl methyl cellulose (HPMC)	189:225	Crosslinking of hydroxypropyl methyl cellulose (HPMC) and acrylic acid (AAc) was carried out at various compositions to develop a high-solid matrix with variable glass transition properties.
33800956	4	18	theme	infrared	751:758	arg1	spectroscopy					760:771	infrared spectroscopy	751:771	infrared spectroscopy (FTIR)	751:778	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	4	18	theme	infrared	751:758	arg1	FTIR					774:777	FTIR	774:777	FTIR	774:777	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	1	19	theme	methyl	203:208	arg1	HPMC					221:224	HPMC	221:224	HPMC	221:224	Crosslinking of hydroxypropyl methyl cellulose (HPMC) and acrylic acid (AAc) was carried out at various compositions to develop a high-solid matrix with variable glass transition properties.
33800956	1	19	theme	methyl	203:208	arg1	cellulose					210:218	hydroxypropyl methyl cellulose	189:218	hydroxypropyl methyl cellulose (HPMC)	189:225	Crosslinking of hydroxypropyl methyl cellulose (HPMC) and acrylic acid (AAc) was carried out at various compositions to develop a high-solid matrix with variable glass transition properties.
33800956	6	20	theme	temperatures	1162:1173	arg1	manipulation					1096:1107	a considerable manipulation	1081:1107	a considerable manipulation of the rheological and calorimetric glass transition temperatures as a function of the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau	1081:1287	There is a considerable manipulation of the rheological and calorimetric glass transition temperatures as a function of the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau.
33800956	1	21	theme	cellulose	210:218	arg1	Crosslinking					173:184	Crosslinking	173:184	Crosslinking of hydroxypropyl methyl cellulose (HPMC) and acrylic acid (AAc)	173:248	Crosslinking of hydroxypropyl methyl cellulose (HPMC) and acrylic acid (AAc) was carried out at various compositions to develop a high-solid matrix with variable glass transition properties.
33800956	0	22	theme	Glass	20:24	arg1	Properties					37:46	the Glass Transition Properties	16:46	the Glass Transition Properties of a High-Solid System Made of Acrylic Acid-N,N'-Methylenebisacrylamide Copolymer	16:128	Manipulation of the Glass Transition Properties of a High-Solid System Made of Acrylic Acid-N,N'-Methylenebisacrylamide Copolymer Grafted on Hydroxypropyl Methyl Cellulose.
33800956	1	23	theme	various	269:275	arg1	compositions					277:288	various compositions	269:288	various compositions	269:288	Crosslinking of hydroxypropyl methyl cellulose (HPMC) and acrylic acid (AAc) was carried out at various compositions to develop a high-solid matrix with variable glass transition properties.
33800956	4	24	theme	differential	816:827	arg1	MDSC					851:854	MDSC	851:854	MDSC	851:854	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	4	24	theme	differential	816:827	arg1	calorimetry					838:848	modulated differential scanning calorimetry	806:848	modulated differential scanning calorimetry (MDSC)	806:855	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	4	25	theme	scanning	829:836	arg1	MDSC					851:854	MDSC	851:854	MDSC	851:854	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	4	25	theme	scanning	829:836	arg1	calorimetry					838:848	modulated differential scanning calorimetry	806:848	modulated differential scanning calorimetry (MDSC)	806:855	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	4	26	theme	modulated	806:814	arg1	MDSC					851:854	MDSC	851:854	MDSC	851:854	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	4	26	theme	modulated	806:814	arg1	calorimetry					838:848	modulated differential scanning calorimetry	806:848	modulated differential scanning calorimetry (MDSC)	806:855	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	2	27	theme	monomers	422:429	arg1	copolymerisation					398:413	the copolymerisation	394:413	the copolymerisation of two monomers, AAc and N,N'-methylenebisacrylamide (MBA) and their grafting onto HMPC	394:501	The matrix was synthesized by the copolymerisation of two monomers, AAc and N,N'-methylenebisacrylamide (MBA) and their grafting onto HMPC.
33800956	6	28	theme	glass	1145:1149	arg1	temperatures					1162:1173	the rheological and calorimetric glass transition temperatures	1112:1173	the rheological and calorimetric glass transition temperatures as a function of the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau	1112:1287	There is a considerable manipulation of the rheological and calorimetric glass transition temperatures as a function of the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau.
33800956	5	29	theme	cohesive	1015:1022	arg1	entity					1039:1044	a cohesive macromolecular entity	1013:1044	a cohesive macromolecular entity that is highly amorphous	1013:1069	The results show the formation of a cohesive macromolecular entity that is highly amorphous.
33800956	5	29	theme	cohesive	1015:1022	arg1	amorphous					1061:1069	amorphous	1061:1069	amorphous	1061:1069	The results show the formation of a cohesive macromolecular entity that is highly amorphous.
33800956	0	30	theme	Properties	37:46	arg1	Manipulation					0:11	Manipulation	0:11	Manipulation of the Glass Transition Properties of a High-Solid System Made of Acrylic Acid-N,N'-Methylenebisacrylamide Copolymer	0:128	Manipulation of the Glass Transition Properties of a High-Solid System Made of Acrylic Acid-N,N'-Methylenebisacrylamide Copolymer Grafted on Hydroxypropyl Methyl Cellulose.
33800956	0	31	theme	Hydroxypropyl	141:153	arg1	Cellulose					162:170	Hydroxypropyl Methyl Cellulose	141:170	Hydroxypropyl Methyl Cellulose	141:170	Manipulation of the Glass Transition Properties of a High-Solid System Made of Acrylic Acid-N,N'-Methylenebisacrylamide Copolymer Grafted on Hydroxypropyl Methyl Cellulose.
33800956	3	32	theme	radical	564:570	arg1	reaction					587:594	the free radical polymerization reaction	555:594	the free radical polymerization reaction	555:594	Potassium persulfate (K2S2O8) was used to initiate the free radical polymerization reaction and tetramethylethylenediamine (TEMED) to accelerate radical polymerisation.
33800956	5	33	theme	macromolecular	1024:1037	arg1	entity					1039:1044	a cohesive macromolecular entity	1013:1044	a cohesive macromolecular entity that is highly amorphous	1013:1069	The results show the formation of a cohesive macromolecular entity that is highly amorphous.
33800956	5	33	theme	macromolecular	1024:1037	arg1	amorphous					1061:1069	amorphous	1061:1069	amorphous	1061:1069	The results show the formation of a cohesive macromolecular entity that is highly amorphous.
33800956	0	34	theme	Transition	26:35	arg1	Properties					37:46	the Glass Transition Properties	16:46	the Glass Transition Properties of a High-Solid System Made of Acrylic Acid-N,N'-Methylenebisacrylamide Copolymer	16:128	Manipulation of the Glass Transition Properties of a High-Solid System Made of Acrylic Acid-N,N'-Methylenebisacrylamide Copolymer Grafted on Hydroxypropyl Methyl Cellulose.
33800956	3	35	theme	polymerization	572:585	arg1	reaction					587:594	the free radical polymerization reaction	555:594	the free radical polymerization reaction	555:594	Potassium persulfate (K2S2O8) was used to initiate the free radical polymerization reaction and tetramethylethylenediamine (TEMED) to accelerate radical polymerisation.
33800956	7	36	theme	networks	1375:1382	arg1	composition					1343:1353	the initial composition	1331:1353	the initial composition of all the HPMC-AAc networks	1331:1382	Complementary TGA work demonstrates that the initial composition of all the HPMC-AAc networks is maintained up to 200 °C, an outcome that bodes well for applications of targeted bioactive compound delivery.
33800956	7	37	theme	Complementary	1290:1302	arg1	work					1308:1311	Complementary TGA work	1290:1311	Complementary TGA work	1290:1311	Complementary TGA work demonstrates that the initial composition of all the HPMC-AAc networks is maintained up to 200 °C, an outcome that bodes well for applications of targeted bioactive compound delivery.
33800956	7	38	dep	200	1404:1406	arg1	to					1401:1402	to	1401:1402	to	1401:1402	Complementary TGA work demonstrates that the initial composition of all the HPMC-AAc networks is maintained up to 200 °C, an outcome that bodes well for applications of targeted bioactive compound delivery.
33800956	2	39	dep	monomers	422:429	arg1	N					440:440	N	440:440	N	440:440	The matrix was synthesized by the copolymerisation of two monomers, AAc and N,N'-methylenebisacrylamide (MBA) and their grafting onto HMPC.
33800956	2	39	dep	monomers	422:429	arg1	AAc					432:434	AAc	432:434	AAc	432:434	The matrix was synthesized by the copolymerisation of two monomers, AAc and N,N'-methylenebisacrylamide (MBA) and their grafting onto HMPC.
33800956	2	39	dep	monomers	422:429	arg1	monomers					422:429	two monomers, AAc and N	418:440	monomers	422:429	The matrix was synthesized by the copolymerisation of two monomers, AAc and N,N'-methylenebisacrylamide (MBA) and their grafting onto HMPC.
33800956	6	40	theme	calorimetric	1132:1143	arg1	temperatures					1162:1173	the rheological and calorimetric glass transition temperatures	1112:1173	the rheological and calorimetric glass transition temperatures as a function of the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau	1112:1287	There is a considerable manipulation of the rheological and calorimetric glass transition temperatures as a function of the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau.
33800956	7	41	theme	targeted	1459:1466	arg1	delivery					1487:1494	targeted bioactive compound delivery	1459:1494	targeted bioactive compound delivery	1459:1494	Complementary TGA work demonstrates that the initial composition of all the HPMC-AAc networks is maintained up to 200 °C, an outcome that bodes well for applications of targeted bioactive compound delivery.
33800956	1	42	theme	high-solid	303:312	arg1	matrix					314:319	a high-solid matrix	301:319	a high-solid matrix with variable glass transition properties	301:361	Crosslinking of hydroxypropyl methyl cellulose (HPMC) and acrylic acid (AAc) was carried out at various compositions to develop a high-solid matrix with variable glass transition properties.
33800956	0	43	theme	Methyl	155:160	arg1	Cellulose					162:170	Hydroxypropyl Methyl Cellulose	141:170	Hydroxypropyl Methyl Cellulose	141:170	Manipulation of the Glass Transition Properties of a High-Solid System Made of Acrylic Acid-N,N'-Methylenebisacrylamide Copolymer Grafted on Hydroxypropyl Methyl Cellulose.
33800956	5	44	theme	entity	1039:1044	arg1	formation					1000:1008	the formation	996:1008	the formation of a cohesive macromolecular entity that is highly amorphous	996:1069	The results show the formation of a cohesive macromolecular entity that is highly amorphous.
33800956	3	45	theme	free	559:562	arg1	radical					564:570	the free radical	555:570	the free radical polymerization reaction	555:594	Potassium persulfate (K2S2O8) was used to initiate the free radical polymerization reaction and tetramethylethylenediamine (TEMED) to accelerate radical polymerisation.
33800956	7	46	theme	initial	1335:1341	arg1	composition					1343:1353	the initial composition	1331:1353	the initial composition of all the HPMC-AAc networks	1331:1382	Complementary TGA work demonstrates that the initial composition of all the HPMC-AAc networks is maintained up to 200 °C, an outcome that bodes well for applications of targeted bioactive compound delivery.
33800956	6	47	theme	extensive	1263:1271	arg1	plateau					1281:1287	an extensive rubbery plateau	1260:1287	an extensive rubbery plateau	1260:1287	There is a considerable manipulation of the rheological and calorimetric glass transition temperatures as a function of the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau.
33800956	6	48	theme	acid	1220:1223	arg1	amount					1196:1201	the amount	1192:1201	the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau	1192:1287	There is a considerable manipulation of the rheological and calorimetric glass transition temperatures as a function of the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau.
33800956	6	48	theme	acid	1220:1223	arg1	acid					1220:1223	added acrylic acid	1206:1223	added acrylic acid	1206:1223	There is a considerable manipulation of the rheological and calorimetric glass transition temperatures as a function of the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau.
33800956	7	49	theme	delivery	1487:1494	arg1	applications					1443:1454	applications	1443:1454	applications of targeted bioactive compound delivery	1443:1494	Complementary TGA work demonstrates that the initial composition of all the HPMC-AAc networks is maintained up to 200 °C, an outcome that bodes well for applications of targeted bioactive compound delivery.
33800956	6	50	theme	acrylic	1212:1218	arg1	acid					1220:1223	added acrylic acid	1206:1223	added acrylic acid	1206:1223	There is a considerable manipulation of the rheological and calorimetric glass transition temperatures as a function of the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau.
33800956	4	51	dep	Fourier	733:739	arg1	transform					741:749	transform	741:749	transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM)	741:976	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	6	52	theme	rheological	1116:1126	arg1	temperatures					1162:1173	the rheological and calorimetric glass transition temperatures	1112:1173	the rheological and calorimetric glass transition temperatures as a function of the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau	1112:1287	There is a considerable manipulation of the rheological and calorimetric glass transition temperatures as a function of the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau.
33800956	6	53	theme	transition	1151:1160	arg1	temperatures					1162:1173	the rheological and calorimetric glass transition temperatures	1112:1173	the rheological and calorimetric glass transition temperatures as a function of the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau	1112:1287	There is a considerable manipulation of the rheological and calorimetric glass transition temperatures as a function of the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau.
33800956	1	54	theme	acrylic	231:237	arg1	AAc					245:247	AAc	245:247	AAc	245:247	Crosslinking of hydroxypropyl methyl cellulose (HPMC) and acrylic acid (AAc) was carried out at various compositions to develop a high-solid matrix with variable glass transition properties.
33800956	1	54	theme	acrylic	231:237	arg1	acid					239:242	acrylic acid	231:242	acrylic acid (AAc)	231:248	Crosslinking of hydroxypropyl methyl cellulose (HPMC) and acrylic acid (AAc) was carried out at various compositions to develop a high-solid matrix with variable glass transition properties.
33800956	4	55	theme	thermogravimetric	906:922	arg1	TGA					934:936	TGA	934:936	TGA	934:936	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	4	55	theme	thermogravimetric	906:922	arg1	analysis					924:931	thermogravimetric analysis	906:931	thermogravimetric analysis (TGA)	906:937	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	3	56	theme	radical	649:655	arg1	polymerisation					657:670	radical polymerisation	649:670	radical polymerisation	649:670	Potassium persulfate (K2S2O8) was used to initiate the free radical polymerization reaction and tetramethylethylenediamine (TEMED) to accelerate radical polymerisation.
33800956	6	57	theme	added	1206:1210	arg1	acid					1220:1223	added acrylic acid	1206:1223	added acrylic acid	1206:1223	There is a considerable manipulation of the rheological and calorimetric glass transition temperatures as a function of the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau.
33800956	1	58	theme	acid	239:242	arg1	Crosslinking					173:184	Crosslinking	173:184	Crosslinking of hydroxypropyl methyl cellulose (HPMC) and acrylic acid (AAc)	173:248	Crosslinking of hydroxypropyl methyl cellulose (HPMC) and acrylic acid (AAc) was carried out at various compositions to develop a high-solid matrix with variable glass transition properties.
33800956	0	59	theme	System	64:69	arg1	Properties					37:46	the Glass Transition Properties	16:46	the Glass Transition Properties of a High-Solid System Made of Acrylic Acid-N,N'-Methylenebisacrylamide Copolymer	16:128	Manipulation of the Glass Transition Properties of a High-Solid System Made of Acrylic Acid-N,N'-Methylenebisacrylamide Copolymer Grafted on Hydroxypropyl Methyl Cellulose.
33800956	3	60	used	used	538:541	arg2	persulfate					514:523	Potassium persulfate	504:523	Potassium persulfate (K2S2O8)	504:532	Potassium persulfate (K2S2O8) was used to initiate the free radical polymerization reaction and tetramethylethylenediamine (TEMED) to accelerate radical polymerisation.
33800956	3	60	used	used	538:541	arg2	K2S2O8					526:531	K2S2O8	526:531	K2S2O8	526:531	Potassium persulfate (K2S2O8) was used to initiate the free radical polymerization reaction and tetramethylethylenediamine (TEMED) to accelerate radical polymerisation.
33800956	7	61	theme	TGA	1304:1306	arg1	work					1308:1311	Complementary TGA work	1290:1311	Complementary TGA work	1290:1311	Complementary TGA work demonstrates that the initial composition of all the HPMC-AAc networks is maintained up to 200 °C, an outcome that bodes well for applications of targeted bioactive compound delivery.
33800956	0	62	theme	High-Solid	53:62	arg1	Copolymer					120:128	N'-Methylenebisacrylamide Copolymer	94:128	N'-Methylenebisacrylamide Copolymer	94:128	Manipulation of the Glass Transition Properties of a High-Solid System Made of Acrylic Acid-N,N'-Methylenebisacrylamide Copolymer Grafted on Hydroxypropyl Methyl Cellulose.
33800956	0	62	theme	High-Solid	53:62	arg1	System					64:69	a High-Solid System	51:69	a High-Solid System Made of Acrylic Acid-N	51:92	Manipulation of the Glass Transition Properties of a High-Solid System Made of Acrylic Acid-N,N'-Methylenebisacrylamide Copolymer Grafted on Hydroxypropyl Methyl Cellulose.
33800956	7	63	theme	bioactive	1468:1476	arg1	delivery					1487:1494	targeted bioactive compound delivery	1459:1494	targeted bioactive compound delivery	1459:1494	Complementary TGA work demonstrates that the initial composition of all the HPMC-AAc networks is maintained up to 200 °C, an outcome that bodes well for applications of targeted bioactive compound delivery.
33800956	6	64	theme	amount	1196:1201	arg1	function					1180:1187	a function	1178:1187	a function of the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau	1178:1287	There is a considerable manipulation of the rheological and calorimetric glass transition temperatures as a function of the amount of added acrylic acid, which is followed upon heating by an extensive rubbery plateau.
33800956	3	65	theme	Potassium	504:512	arg1	K2S2O8					526:531	K2S2O8	526:531	K2S2O8	526:531	Potassium persulfate (K2S2O8) was used to initiate the free radical polymerization reaction and tetramethylethylenediamine (TEMED) to accelerate radical polymerisation.
33800956	3	65	theme	Potassium	504:512	arg1	persulfate					514:523	Potassium persulfate	504:523	Potassium persulfate (K2S2O8)	504:532	Potassium persulfate (K2S2O8) was used to initiate the free radical polymerization reaction and tetramethylethylenediamine (TEMED) to accelerate radical polymerisation.
33800956	4	66	theme	scanning	943:950	arg1	microscopy					961:970	scanning electron microscopy	943:970	scanning electron microscopy (SEM)	943:976	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
33800956	4	66	theme	scanning	943:950	arg1	SEM					973:975	SEM	973:975	SEM	973:975	Structural properties of the network were investigated with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), modulated differential scanning calorimetry (MDSC), small-deformation dynamic oscillation in-shear, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).
32919575	6	0	theme	CTL-hydrogel	875:886	arg1	advantages					857:866	the advantages	853:866	the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules	853:1089	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	1	1	theme	joint	150:154	arg1	functionality					156:168	joint functionality	150:168	joint functionality	150:168	Osteoarthritis (OA) is a chronic disease affecting joint functionality and often managed with hyaluronic acid (HA) administration.
32919575	6	2	theme	innovative	940:949	arg1	one					988:990	one	988:990	one	988:990	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	6	2	theme	innovative	940:949	arg1	application					951:961	an innovative application	937:961	an innovative application	937:961	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	6	2	theme	innovative	940:949	arg1	component					1004:1012	the main component	995:1012	the main component of the proposed hydrogel system and not used in mixture with other molecules	995:1089	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	5	3	theme	Degradation	694:704	arg1	studies					706:712	Degradation studies	694:712	Degradation studies carried out over 24 h	694:734	Degradation studies carried out over 24 h pointed out its higher resistance to chemical degradation with respect to HA samples.
32919575	0	4	from	chitosan	46:53	arg1	osteoarthritis					83:96	osteoarthritis	83:96	osteoarthritis	83:96	A hydrogel system based on a lactose-modified chitosan for viscosupplementation in osteoarthritis.
32919575	4	5	theme	cytokines	660:668	arg1	release					670:676	cytokines release	660:676	cytokines release by macrophages	660:691	The selected CTL-hydrogel showed biocompatibility and antioxidant activity in vitro, and it did not influence cytokines release by macrophages.
32919575	6	6	dep	CTL-hydrogel	875:886	arg1	address					891:897	address	891:897	to address the treatment of OA	888:917	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	6	6	dep	CTL-hydrogel	875:886	arg1	shed					923:926	shed	923:926	shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules	923:1089	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	6	7	theme	proposed	1021:1028	arg1	system					1039:1044	the proposed hydrogel system	1017:1044	the proposed hydrogel system	1017:1044	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	6	8	theme	main	999:1002	arg1	component					1004:1012	the main component	995:1012	the main component of the proposed hydrogel system and not used in mixture with other molecules	995:1089	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	6	9	theme	not	1050:1052	arg1	component					1004:1012	the main component	995:1012	the main component of the proposed hydrogel system and not used in mixture with other molecules	995:1089	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	6	10	theme	polymer	970:976	arg1	one					988:990	one	988:990	one	988:990	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	6	10	theme	polymer	970:976	arg1	application					951:961	an innovative application	937:961	an innovative application	937:961	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	6	10	theme	polymer	970:976	arg1	component					1004:1012	the main component	995:1012	the main component of the proposed hydrogel system and not used in mixture with other molecules	995:1089	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	6	11	theme	component	1004:1012	arg1	one					988:990	one	988:990	one	988:990	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	6	11	theme	component	1004:1012	arg1	application					951:961	an innovative application	937:961	an innovative application	937:961	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	6	11	theme	component	1004:1012	arg1	component					1004:1012	the main component	995:1012	the main component of the proposed hydrogel system and not used in mixture with other molecules	995:1089	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	0	12	theme	hydrogel	2:9	arg1	system					11:16	A hydrogel system	0:16	A hydrogel system	0:16	A hydrogel system based on a lactose-modified chitosan for viscosupplementation in osteoarthritis.
32919575	3	13	theme	tens	533:536	arg1	order					524:528	the order	520:528	the order of tens of Pascal	520:546	The rheological characterization allowed to identify a composition whose properties were in line with those of commercial products (in the order of tens of Pascal).
32919575	3	14	theme	rheological	389:399	arg1	characterization					401:416	The rheological characterization	385:416	The rheological characterization	385:416	The rheological characterization allowed to identify a composition whose properties were in line with those of commercial products (in the order of tens of Pascal).
32919575	5	15	theme	chemical	773:780	arg1	degradation					782:792	chemical degradation	773:792	chemical degradation	773:792	Degradation studies carried out over 24 h pointed out its higher resistance to chemical degradation with respect to HA samples.
32919575	3	16	with	line	477:480	arg1	those					487:491	those	487:491	those	487:491	The rheological characterization allowed to identify a composition whose properties were in line with those of commercial products (in the order of tens of Pascal).
32919575	6	17	theme	other	1075:1079	arg1	molecules					1081:1089	other molecules	1075:1089	other molecules	1075:1089	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	1	18	dep	disease	132:138	arg1	managed					180:186	managed	180:186	often managed with hyaluronic acid (HA) administration	174:227	Osteoarthritis (OA) is a chronic disease affecting joint functionality and often managed with hyaluronic acid (HA) administration.
32919575	1	18	dep	disease	132:138	arg1	affecting					140:148	affecting	140:148	affecting joint functionality	140:168	Osteoarthritis (OA) is a chronic disease affecting joint functionality and often managed with hyaluronic acid (HA) administration.
32919575	4	19	theme	antioxidant	604:614	arg1	activity					616:623	antioxidant activity	604:623	antioxidant activity	604:623	The selected CTL-hydrogel showed biocompatibility and antioxidant activity in vitro, and it did not influence cytokines release by macrophages.
32919575	2	20	with	reticulated	299:309	arg1	acid					322:325	boric acid	316:325	boric acid	316:325	In this study, a hydrogel based on a lactose-modified chitosan (CTL) reticulated with boric acid has been developed as a viscosupplement for OA treatment.
32919575	2	21	theme	lactose-modified	267:282	arg1	chitosan					284:291	a lactose-modified chitosan	265:291	a lactose-modified chitosan (CTL) reticulated with boric acid	265:325	In this study, a hydrogel based on a lactose-modified chitosan (CTL) reticulated with boric acid has been developed as a viscosupplement for OA treatment.
32919575	2	21	theme	lactose-modified	267:282	arg1	CTL					294:296	CTL	294:296	CTL	294:296	In this study, a hydrogel based on a lactose-modified chitosan (CTL) reticulated with boric acid has been developed as a viscosupplement for OA treatment.
32919575	1	22	theme	acid	204:207	arg1	administration					214:227	hyaluronic acid (HA) administration	193:227	hyaluronic acid (HA) administration	193:227	Osteoarthritis (OA) is a chronic disease affecting joint functionality and often managed with hyaluronic acid (HA) administration.
32919575	6	23	theme	OA	916:917	arg1	treatment					903:911	the treatment	899:911	the treatment of OA	899:917	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	5	24	theme	HA	810:811	arg1	samples					813:819	HA samples	810:819	HA samples	810:819	Degradation studies carried out over 24 h pointed out its higher resistance to chemical degradation with respect to HA samples.
32919575	2	25	theme	OA	371:372	arg1	treatment					374:382	OA treatment	371:382	OA treatment	371:382	In this study, a hydrogel based on a lactose-modified chitosan (CTL) reticulated with boric acid has been developed as a viscosupplement for OA treatment.
32919575	5	26	theme	higher	752:757	arg1	resistance					759:768	its higher resistance	748:768	its higher resistance to chemical degradation	748:792	Degradation studies carried out over 24 h pointed out its higher resistance to chemical degradation with respect to HA samples.
32919575	6	27	with	mixture	1062:1068	arg1	molecules					1081:1089	other molecules	1075:1089	other molecules	1075:1089	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	3	28	theme	Pascal	541:546	arg1	tens					533:536	tens	533:536	tens of Pascal	533:546	The rheological characterization allowed to identify a composition whose properties were in line with those of commercial products (in the order of tens of Pascal).
32919575	2	29	theme	boric	316:320	arg1	acid					322:325	boric acid	316:325	boric acid	316:325	In this study, a hydrogel based on a lactose-modified chitosan (CTL) reticulated with boric acid has been developed as a viscosupplement for OA treatment.
32919575	0	30	theme	lactose-modified	29:44	arg1	chitosan					46:53	a lactose-modified chitosan	27:53	a lactose-modified chitosan for viscosupplementation in osteoarthritis	27:96	A hydrogel system based on a lactose-modified chitosan for viscosupplementation in osteoarthritis.
32919575	6	31	dep	not	1050:1052	arg1	used					1054:1057	used	1054:1057	used in mixture with other molecules	1054:1089	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	3	32	dep	identify	429:436	arg1	order					524:528	the order	520:528	the order of tens of Pascal	520:546	The rheological characterization allowed to identify a composition whose properties were in line with those of commercial products (in the order of tens of Pascal).
32919575	2	33	theme	reticulated	299:309	arg1	chitosan					284:291	a lactose-modified chitosan	265:291	a lactose-modified chitosan (CTL) reticulated with boric acid	265:325	In this study, a hydrogel based on a lactose-modified chitosan (CTL) reticulated with boric acid has been developed as a viscosupplement for OA treatment.
32919575	2	33	theme	reticulated	299:309	arg1	CTL					294:296	CTL	294:296	CTL	294:296	In this study, a hydrogel based on a lactose-modified chitosan (CTL) reticulated with boric acid has been developed as a viscosupplement for OA treatment.
32919575	1	34	theme	chronic	124:130	arg1	Osteoarthritis					99:112	Osteoarthritis	99:112	Osteoarthritis (OA)	99:117	Osteoarthritis (OA) is a chronic disease affecting joint functionality and often managed with hyaluronic acid (HA) administration.
32919575	1	34	theme	chronic	124:130	arg1	disease					132:138	a chronic disease	122:138	a chronic disease affecting joint functionality and often managed with hyaluronic acid (HA) administration	122:227	Osteoarthritis (OA) is a chronic disease affecting joint functionality and often managed with hyaluronic acid (HA) administration.
32919575	4	35	theme	selected	554:561	arg1	CTL-hydrogel					563:574	The selected CTL-hydrogel	550:574	The selected CTL-hydrogel	550:574	The selected CTL-hydrogel showed biocompatibility and antioxidant activity in vitro, and it did not influence cytokines release by macrophages.
32919575	6	36	theme	hydrogel	1030:1037	arg1	system					1039:1044	the proposed hydrogel system	1017:1044	the proposed hydrogel system	1017:1044	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	1	37	theme	hyaluronic	193:202	arg1	acid					204:207	hyaluronic acid	193:207	hyaluronic acid (HA) administration	193:227	Osteoarthritis (OA) is a chronic disease affecting joint functionality and often managed with hyaluronic acid (HA) administration.
32919575	1	37	theme	hyaluronic	193:202	arg1	HA					210:211	HA	210:211	HA	210:211	Osteoarthritis (OA) is a chronic disease affecting joint functionality and often managed with hyaluronic acid (HA) administration.
32919575	6	38	theme	system	1039:1044	arg1	component					1004:1012	the main component	995:1012	the main component of the proposed hydrogel system and not used in mixture with other molecules	995:1089	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	6	39	theme	CTL	966:968	arg1	polymer					970:976	CTL polymer	966:976	CTL polymer	966:976	Overall, this study underlines the advantages of the CTL-hydrogel to address the treatment of OA and shed light on an innovative application of CTL polymer, which is one of the main component of the proposed hydrogel system and not used in mixture with other molecules.
32919575	3	40	theme	commercial	496:505	arg1	products					507:514	commercial products	496:514	commercial products	496:514	The rheological characterization allowed to identify a composition whose properties were in line with those of commercial products (in the order of tens of Pascal).
32758805	0	0	theme	pearled	79:85	arg1	composition					94:104	pearled kernel composition	79:104	pearled kernel composition	79:104	Distributions of nutrients and avenanthramides within oat grain and effects on pearled kernel composition.
32758805	5	1	dep	increased	894:902	arg1	decreased					910:918	decreased	910:918	decreased	910:918	In Lamont oat, the four AVAs decreased, but in Reins oat, AVA 2p decreased while 2c, 2f and 5p increased, then decreased.
32758805	2	2	from	2f	372:373	arg1	fractions					397:405	processing fractions	386:405	processing fractions	386:405	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	2	2	from	2f	372:373	arg1	grains					420:425	starting grains	411:425	starting grains	411:425	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	4	3	theme	other	695:699	arg1	carbohydrates					701:713	other carbohydrates	695:713	other carbohydrates	695:713	In both varieties, from the surface to inner endosperms, protein and oil increased then decreased; ash and other carbohydrates decreased; starch increased; and beta-glucan unchanged except for the surface area.
32758805	6	4	theme	different	1082:1090	arg1	composition					1096:1106	a different AVA composition	1080:1106	a different AVA composition	1080:1106	Compared to whole grain, pearled oats not only contained lower AVAs, protein, oil, ash, and other carbohydrates and higher beta-glucan and starch but also had a different AVA composition.
32758805	4	5	theme	inner	627:631	arg1	endosperms					633:642	inner endosperms	627:642	inner endosperms	627:642	In both varieties, from the surface to inner endosperms, protein and oil increased then decreased; ash and other carbohydrates decreased; starch increased; and beta-glucan unchanged except for the surface area.
32758805	3	6	theme	individual	571:580	arg1	AVAs					582:585	individual AVAs	571:585	individual AVAs	571:585	Results show that distribution patterns of nutrients varied with individual nutrients, but those of AVAs varied with variety and individual AVAs.
32758805	0	7	theme	kernel	87:92	arg1	composition					94:104	pearled kernel composition	79:104	pearled kernel composition	79:104	Distributions of nutrients and avenanthramides within oat grain and effects on pearled kernel composition.
32758805	0	8	from	grain	58:62	arg1	composition					94:104	pearled kernel composition	79:104	pearled kernel composition	79:104	Distributions of nutrients and avenanthramides within oat grain and effects on pearled kernel composition.
32758805	6	9	theme	other	1013:1017	arg1	carbohydrates					1019:1031	other carbohydrates	1013:1031	other carbohydrates	1013:1031	Compared to whole grain, pearled oats not only contained lower AVAs, protein, oil, ash, and other carbohydrates and higher beta-glucan and starch but also had a different AVA composition.
32758805	4	10	dep	increased	661:669	arg1	decreased					676:684	decreased	676:684	increased then decreased	661:684	In both varieties, from the surface to inner endosperms, protein and oil increased then decreased; ash and other carbohydrates decreased; starch increased; and beta-glucan unchanged except for the surface area.
32758805	2	11	from	fractions	397:405	arg1	Contents					251:258	Contents	251:258	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains	251:425	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	6	12	theme	pearled	946:952	arg1	oats					954:957	pearled oats	946:957	pearled oats	946:957	Compared to whole grain, pearled oats not only contained lower AVAs, protein, oil, ash, and other carbohydrates and higher beta-glucan and starch but also had a different AVA composition.
32758805	2	13	from	grains	420:425	arg1	Contents					251:258	Contents	251:258	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains	251:425	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	6	14	theme	AVA	1092:1094	arg1	composition					1096:1106	a different AVA composition	1080:1106	a different AVA composition	1080:1106	Compared to whole grain, pearled oats not only contained lower AVAs, protein, oil, ash, and other carbohydrates and higher beta-glucan and starch but also had a different AVA composition.
32758805	2	15	theme	protein	274:280	arg1	ash					309:311	protein, oil, starch, beta-glucan, ash and other carbohydrates	274:335	ash	309:311	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	2	15	theme	protein	274:280	arg1	nutrients					263:271	nutrients	263:271	nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates)	263:336	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	2	16	theme	2p	364:365	arg1	Contents					251:258	Contents	251:258	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains	251:425	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	2	17	theme	processing	386:395	arg1	fractions					397:405	processing fractions	386:405	processing fractions	386:405	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	4	18	theme	surface	785:791	arg1	area					793:796	the surface area	781:796	the surface area	781:796	In both varieties, from the surface to inner endosperms, protein and oil increased then decreased; ash and other carbohydrates decreased; starch increased; and beta-glucan unchanged except for the surface area.
32758805	5	19	theme	Reins	846:850	arg1	oat					852:854	Reins oat	846:854	Reins oat	846:854	In Lamont oat, the four AVAs decreased, but in Reins oat, AVA 2p decreased while 2c, 2f and 5p increased, then decreased.
32758805	2	20	theme	AVA	359:361	arg1	2p					364:365	avenanthramides (AVA) 2p	342:365	avenanthramides (AVA) 2p	342:365	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	3	21	theme	individual	507:516	arg1	nutrients					518:526	individual nutrients	507:526	individual nutrients	507:526	Results show that distribution patterns of nutrients varied with individual nutrients, but those of AVAs varied with variety and individual AVAs.
32758805	2	22	from	Contents	251:258	arg1	fractions					397:405	processing fractions	386:405	processing fractions	386:405	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	2	22	from	Contents	251:258	arg1	grains					420:425	starting grains	411:425	starting grains	411:425	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	0	23	theme	nutrients	17:25	arg1	Distributions					0:12	Distributions	0:12	Distributions of nutrients and avenanthramides within oat grain and effects on pearled kernel composition.	0:105	Distributions of nutrients and avenanthramides within oat grain and effects on pearled kernel composition.
32758805	1	24	theme	Hulled	107:112	arg1	Reins					114:118	Hulled Reins	107:118	Hulled Reins	107:118	Hulled Reins and hulless Lamont oats were dehulled and/or sequentially abraded to produce ten pearling fines and corresponding pearled kernels.
32758805	1	25	theme	pearling	201:208	arg1	fines					210:214	ten pearling fines	197:214	ten pearling fines	197:214	Hulled Reins and hulless Lamont oats were dehulled and/or sequentially abraded to produce ten pearling fines and corresponding pearled kernels.
32758805	6	26	contain	had	1076:1078	arg1	oats					954:957	pearled oats	946:957	pearled oats	946:957	Compared to whole grain, pearled oats not only contained lower AVAs, protein, oil, ash, and other carbohydrates and higher beta-glucan and starch but also had a different AVA composition.
32758805	6	26	contain	had	1076:1078	arg2	composition					1096:1106	a different AVA composition	1080:1106	a different AVA composition	1080:1106	Compared to whole grain, pearled oats not only contained lower AVAs, protein, oil, ash, and other carbohydrates and higher beta-glucan and starch but also had a different AVA composition.
32758805	2	27	from	nutrients	263:271	arg1	fractions					397:405	processing fractions	386:405	processing fractions	386:405	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	2	27	from	nutrients	263:271	arg1	grains					420:425	starting grains	411:425	starting grains	411:425	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	0	28	theme	avenanthramides	31:45	arg1	Distributions					0:12	Distributions	0:12	Distributions of nutrients and avenanthramides within oat grain and effects on pearled kernel composition.	0:105	Distributions of nutrients and avenanthramides within oat grain and effects on pearled kernel composition.
32758805	2	29	theme	starting	411:418	arg1	grains					420:425	starting grains	411:425	starting grains	411:425	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	0	30	from	effects	68:74	arg1	composition					94:104	pearled kernel composition	79:104	pearled kernel composition	79:104	Distributions of nutrients and avenanthramides within oat grain and effects on pearled kernel composition.
32758805	6	31	theme	AVAs	984:987	arg1	ash					1004:1006	AVAs, protein, oil, ash, and other carbohydrates	984:1031	ash	1004:1006	Compared to whole grain, pearled oats not only contained lower AVAs, protein, oil, ash, and other carbohydrates and higher beta-glucan and starch but also had a different AVA composition.
32758805	6	32	dep	higher	1037:1042	arg1	beta-glucan					1044:1054	beta-glucan	1044:1054	beta-glucan	1044:1054	Compared to whole grain, pearled oats not only contained lower AVAs, protein, oil, ash, and other carbohydrates and higher beta-glucan and starch but also had a different AVA composition.
32758805	6	32	dep	higher	1037:1042	arg1	starch					1060:1065	starch	1060:1065	starch	1060:1065	Compared to whole grain, pearled oats not only contained lower AVAs, protein, oil, ash, and other carbohydrates and higher beta-glucan and starch but also had a different AVA composition.
32758805	0	33	theme	oat	54:56	arg1	grain					58:62	oat grain	54:62	oat grain	54:62	Distributions of nutrients and avenanthramides within oat grain and effects on pearled kernel composition.
32758805	6	34	theme	whole	933:937	arg1	grain					939:943	whole grain	933:943	whole grain	933:943	Compared to whole grain, pearled oats not only contained lower AVAs, protein, oil, ash, and other carbohydrates and higher beta-glucan and starch but also had a different AVA composition.
32758805	2	35	theme	other	317:321	arg1	carbohydrates					323:335	other carbohydrates	317:335	other carbohydrates	317:335	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	2	35	theme	other	317:321	arg1	nutrients					263:271	nutrients	263:271	nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates)	263:336	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	2	36	from	2p	364:365	arg1	fractions					397:405	processing fractions	386:405	processing fractions	386:405	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	2	36	from	2p	364:365	arg1	grains					420:425	starting grains	411:425	starting grains	411:425	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	1	37	theme	hulless	124:130	arg1	oats					139:142	hulless Lamont oats	124:142	hulless Lamont oats	124:142	Hulled Reins and hulless Lamont oats were dehulled and/or sequentially abraded to produce ten pearling fines and corresponding pearled kernels.
32758805	5	38	theme	Lamont	802:807	arg1	oat					809:811	Lamont oat	802:811	Lamont oat	802:811	In Lamont oat, the four AVAs decreased, but in Reins oat, AVA 2p decreased while 2c, 2f and 5p increased, then decreased.
32758805	1	39	theme	corresponding	220:232	arg1	kernels					242:248	corresponding pearled kernels	220:248	corresponding pearled kernels	220:248	Hulled Reins and hulless Lamont oats were dehulled and/or sequentially abraded to produce ten pearling fines and corresponding pearled kernels.
32758805	3	40	theme	nutrients	485:493	arg1	patterns					473:480	distribution patterns	460:480	distribution patterns of nutrients	460:493	Results show that distribution patterns of nutrients varied with individual nutrients, but those of AVAs varied with variety and individual AVAs.
32758805	2	41	theme	nutrients	263:271	arg1	Contents					251:258	Contents	251:258	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains	251:425	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	1	42	theme	Lamont	132:137	arg1	oats					139:142	hulless Lamont oats	124:142	hulless Lamont oats	124:142	Hulled Reins and hulless Lamont oats were dehulled and/or sequentially abraded to produce ten pearling fines and corresponding pearled kernels.
32758805	1	43	theme	pearled	234:240	arg1	kernels					242:248	corresponding pearled kernels	220:248	corresponding pearled kernels	220:248	Hulled Reins and hulless Lamont oats were dehulled and/or sequentially abraded to produce ten pearling fines and corresponding pearled kernels.
32758805	6	44	contain	contained	968:976	arg1	oats					954:957	pearled oats	946:957	pearled oats	946:957	Compared to whole grain, pearled oats not only contained lower AVAs, protein, oil, ash, and other carbohydrates and higher beta-glucan and starch but also had a different AVA composition.
32758805	6	44	contain	contained	968:976	arg2	composition					1096:1106	a different AVA composition	1080:1106	a different AVA composition	1080:1106	Compared to whole grain, pearled oats not only contained lower AVAs, protein, oil, ash, and other carbohydrates and higher beta-glucan and starch but also had a different AVA composition.
32758805	2	45	from	2c	368:369	arg1	fractions					397:405	processing fractions	386:405	processing fractions	386:405	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	2	45	from	2c	368:369	arg1	grains					420:425	starting grains	411:425	starting grains	411:425	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	3	46	theme	distribution	460:471	arg1	patterns					473:480	distribution patterns	460:480	distribution patterns of nutrients	460:493	Results show that distribution patterns of nutrients varied with individual nutrients, but those of AVAs varied with variety and individual AVAs.
32758805	2	47	from	5p	380:381	arg1	fractions					397:405	processing fractions	386:405	processing fractions	386:405	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	2	47	from	5p	380:381	arg1	grains					420:425	starting grains	411:425	starting grains	411:425	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
32758805	6	48	dep	lower	978:982	arg1	protein					990:996	AVAs, protein, oil, ash, and other carbohydrates	984:1031	protein	990:996	Compared to whole grain, pearled oats not only contained lower AVAs, protein, oil, ash, and other carbohydrates and higher beta-glucan and starch but also had a different AVA composition.
32758805	6	48	dep	lower	978:982	arg1	ash					1004:1006	AVAs, protein, oil, ash, and other carbohydrates	984:1031	ash	1004:1006	Compared to whole grain, pearled oats not only contained lower AVAs, protein, oil, ash, and other carbohydrates and higher beta-glucan and starch but also had a different AVA composition.
32758805	6	48	dep	lower	978:982	arg1	oil					999:1001	AVAs, protein, oil, ash, and other carbohydrates	984:1031	oil	999:1001	Compared to whole grain, pearled oats not only contained lower AVAs, protein, oil, ash, and other carbohydrates and higher beta-glucan and starch but also had a different AVA composition.
32758805	6	48	dep	lower	978:982	arg1	carbohydrates					1019:1031	other carbohydrates	1013:1031	other carbohydrates	1013:1031	Compared to whole grain, pearled oats not only contained lower AVAs, protein, oil, ash, and other carbohydrates and higher beta-glucan and starch but also had a different AVA composition.
32758805	2	49	theme	avenanthramides	342:356	arg1	2p					364:365	avenanthramides (AVA) 2p	342:365	avenanthramides (AVA) 2p	342:365	Contents of nutrients (protein, oil, starch, beta-glucan, ash and other carbohydrates) and avenanthramides (AVA) 2p, 2c, 2f, and 5p in processing fractions and starting grains were measured.
34323161	6	0	dep	bagasses	1131:1138	arg1	enriched					1140:1147	enriched	1140:1147	bagasses enriched the beneficial genus Bifidobacterium in the obese microbiota	1131:1208	Cassava bagasses enriched the beneficial genus Bifidobacterium in the obese microbiota.
34323161	6	1	theme	beneficial	1153:1162	arg1	Bifidobacterium					1170:1184	the beneficial genus Bifidobacterium	1149:1184	the beneficial genus Bifidobacterium	1149:1184	Cassava bagasses enriched the beneficial genus Bifidobacterium in the obese microbiota.
34323161	10	2	theme	highest	1628:1634	arg1	diversity					1636:1644	diversity	1636:1644	diversity	1636:1644	Nevertheless, passion fruit peel fermentation resulted in a microbiota with the highest diversity and evenness, a positive trait regarding host health.
34323161	10	2	theme	highest	1628:1634	arg1	trait					1671:1675	a positive trait	1660:1675	a positive trait regarding host health	1660:1697	Nevertheless, passion fruit peel fermentation resulted in a microbiota with the highest diversity and evenness, a positive trait regarding host health.
34323161	12	3	theme	non-invasive	1907:1918	arg1	way					1920:1922	an inexpensive and non-invasive way	1888:1922	an inexpensive and non-invasive way to be used as a dietary intervention to improve health, as they were shown here to drive the composition of the obese microbiota to a healthier profile	1888:2074	They are an inexpensive and non-invasive way to be used as a dietary intervention to improve health, as they were shown here to drive the composition of the obese microbiota to a healthier profile.
34323161	12	3	theme	non-invasive	1907:1918	arg1	They					1879:1882	They	1879:1882	They	1879:1882	They are an inexpensive and non-invasive way to be used as a dietary intervention to improve health, as they were shown here to drive the composition of the obese microbiota to a healthier profile.
34323161	4	4	theme	microbiota	773:782	arg1	clustering					744:753	a clear separate clustering	727:753	a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation	727:854	Principal Coordinate Analysis of the microbiota showed evidence of a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation, and instead, distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin) were present.
34323161	1	5	theme	faecal	369:374	arg1	microbiota					376:385	faecal microbiota	369:385	faecal microbiota from lean or obese people	369:411	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	10	6	theme	positive	1662:1669	arg1	diversity					1636:1644	diversity	1636:1644	diversity	1636:1644	Nevertheless, passion fruit peel fermentation resulted in a microbiota with the highest diversity and evenness, a positive trait regarding host health.
34323161	10	6	theme	positive	1662:1669	arg1	trait					1671:1675	a positive trait	1660:1675	a positive trait regarding host health	1660:1697	Nevertheless, passion fruit peel fermentation resulted in a microbiota with the highest diversity and evenness, a positive trait regarding host health.
34323161	4	7	theme	separate	735:742	arg1	clustering					744:753	a clear separate clustering	727:753	a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation	727:854	Principal Coordinate Analysis of the microbiota showed evidence of a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation, and instead, distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin) were present.
34323161	12	8	theme	inexpensive	1891:1901	arg1	way					1920:1922	an inexpensive and non-invasive way	1888:1922	an inexpensive and non-invasive way to be used as a dietary intervention to improve health, as they were shown here to drive the composition of the obese microbiota to a healthier profile	1888:2074	They are an inexpensive and non-invasive way to be used as a dietary intervention to improve health, as they were shown here to drive the composition of the obese microbiota to a healthier profile.
34323161	12	8	theme	inexpensive	1891:1901	arg1	They					1879:1882	They	1879:1882	They	1879:1882	They are an inexpensive and non-invasive way to be used as a dietary intervention to improve health, as they were shown here to drive the composition of the obese microbiota to a healthier profile.
34323161	7	9	theme	obese	1267:1271	arg1	microbiota					1273:1282	the obese microbiota	1263:1282	the obese microbiota	1263:1282	The production of total SCFA by cassava bagasses by the obese microbiota was higher than for control medium and inulin.
34323161	11	10	theme	food-by-products	1726:1741	arg1	step					1765:1768	an important step	1752:1768	an important step to tackle obesity and decrease the waste of valuable food material and consequently environmental pollution	1752:1876	In conclusion, the use of food-by-products could be an important step to tackle obesity and decrease the waste of valuable food material and consequently environmental pollution.
34323161	11	10	theme	food-by-products	1726:1741	arg1	use					1719:1721	the use	1715:1721	the use of food-by-products	1715:1741	In conclusion, the use of food-by-products could be an important step to tackle obesity and decrease the waste of valuable food material and consequently environmental pollution.
34323161	7	11	theme	cassava	1243:1249	arg1	bagasses					1251:1258	cassava bagasses	1243:1258	cassava bagasses	1243:1258	The production of total SCFA by cassava bagasses by the obese microbiota was higher than for control medium and inulin.
34323161	1	12	theme	possible	321:328	arg1	differences					330:340	possible differences	321:340	possible differences in fermentation	321:356	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	11	13	theme	environmental	1854:1866	arg1	pollution					1868:1876	environmental pollution	1854:1876	environmental pollution	1854:1876	In conclusion, the use of food-by-products could be an important step to tackle obesity and decrease the waste of valuable food material and consequently environmental pollution.
34323161	1	14	theme	study	100:104	arg1	aim					89:91	The aim	85:91	The aim of the study	85:104	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	7	15	theme	SCFA	1235:1238	arg1	higher					1288:1293	higher	1288:1293	higher	1288:1293	The production of total SCFA by cassava bagasses by the obese microbiota was higher than for control medium and inulin.
34323161	7	15	theme	SCFA	1235:1238	arg1	production					1215:1224	The production	1211:1224	The production of total SCFA by cassava bagasses by the obese microbiota	1211:1282	The production of total SCFA by cassava bagasses by the obese microbiota was higher than for control medium and inulin.
34323161	3	16	theme	by-products	524:534	arg1	effects					509:515	The effects	505:515	The effects of the by-products on the dynamics of the gut microbiota	505:572	The effects of the by-products on the dynamics of the gut microbiota differed according to the type of microbiota, as well as the type of by-product used.
34323161	1	17	from	differences	330:340	arg1	fermentation					345:356	fermentation	345:356	fermentation	345:356	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	1	18	theme	prebiotic	139:147	arg1	effects					149:155	the potential prebiotic effects	125:155	the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon	125:304	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	8	19	theme	butyrate-producing	1376:1393	arg1	Coprococcus					1401:1411	the butyrate-producing genus Coprococcus	1372:1411	the butyrate-producing genus Coprococcus	1372:1411	Orange bagasses stimulated the growth of the butyrate-producing genus Coprococcus.
34323161	2	20	theme	by-products	434:444	arg1	Fermentation					414:425	Fermentation	414:425	Fermentation of the by-products	414:444	Fermentation of the by-products was compared to a control medium and the prebiotic inulin.
34323161	8	21	theme	Coprococcus	1401:1411	arg1	growth					1362:1367	the growth	1358:1367	the growth of the butyrate-producing genus Coprococcus	1358:1411	Orange bagasses stimulated the growth of the butyrate-producing genus Coprococcus.
34323161	4	22	theme	Coordinate	670:679	arg1	Analysis					681:688	Principal Coordinate Analysis	660:688	Principal Coordinate Analysis of the microbiota	660:706	Principal Coordinate Analysis of the microbiota showed evidence of a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation, and instead, distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin) were present.
34323161	10	23	theme	fruit	1570:1574	arg1	fermentation					1581:1592	passion fruit peel fermentation	1562:1592	passion fruit peel fermentation	1562:1592	Nevertheless, passion fruit peel fermentation resulted in a microbiota with the highest diversity and evenness, a positive trait regarding host health.
34323161	1	24	theme	proximal	291:298	arg1	colon					300:304	the proximal colon	287:304	the proximal colon	287:304	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	11	25	dep	step	1765:1768	arg1	pollution					1868:1876	environmental pollution	1854:1876	environmental pollution	1854:1876	In conclusion, the use of food-by-products could be an important step to tackle obesity and decrease the waste of valuable food material and consequently environmental pollution.
34323161	11	25	dep	step	1765:1768	arg1	tackle					1773:1778	tackle	1773:1778	tackle obesity	1773:1786	In conclusion, the use of food-by-products could be an important step to tackle obesity and decrease the waste of valuable food material and consequently environmental pollution.
34323161	11	25	dep	step	1765:1768	arg1	decrease					1792:1799	decrease	1792:1799	decrease the waste of valuable food material	1792:1835	In conclusion, the use of food-by-products could be an important step to tackle obesity and decrease the waste of valuable food material and consequently environmental pollution.
34323161	0	26	theme	Lean	0:3	arg1	microbiota					15:24	Lean and obese microbiota	0:24	Lean and obese microbiota: differences in in vitro fermentation of food-by-products.	0:83	Lean and obese microbiota: differences in in vitro fermentation of food-by-products.
34323161	1	27	theme	passion	226:232	arg1	peel					240:243	passion fruit peel	226:243	passion fruit peel (n=3)	226:249	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	1	27	theme	passion	226:232	arg1	food-by-products					160:175	food-by-products	160:175	food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon	160:304	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	1	27	theme	passion	226:232	arg1	n=3					246:248	n=3	246:248	n=3	246:248	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	4	28	theme	by-products	935:945	arg1	composition					916:926	primary carbohydrate composition	895:926	primary carbohydrate composition of the by-products (starch, fructan and pectin)	895:974	Principal Coordinate Analysis of the microbiota showed evidence of a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation, and instead, distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin) were present.
34323161	11	29	from	step	1765:1768	arg1	conclusion					1703:1712	conclusion	1703:1712	conclusion	1703:1712	In conclusion, the use of food-by-products could be an important step to tackle obesity and decrease the waste of valuable food material and consequently environmental pollution.
34323161	12	30	theme	healthier	2058:2066	arg1	profile					2068:2074	a healthier profile	2056:2074	a healthier profile	2056:2074	They are an inexpensive and non-invasive way to be used as a dietary intervention to improve health, as they were shown here to drive the composition of the obese microbiota to a healthier profile.
34323161	11	31	theme	material	1828:1835	arg1	waste					1805:1809	the waste	1801:1809	the waste of valuable food material	1801:1835	In conclusion, the use of food-by-products could be an important step to tackle obesity and decrease the waste of valuable food material and consequently environmental pollution.
34323161	0	32	theme	obese	9:13	arg1	microbiota					15:24	Lean and obese microbiota	0:24	Lean and obese microbiota: differences in in vitro fermentation of food-by-products.	0:83	Lean and obese microbiota: differences in in vitro fermentation of food-by-products.
34323161	5	33	theme	healthier	1059:1067	arg1	profile					1069:1075	a healthier profile	1057:1075	a healthier profile	1057:1075	This is evidence that the substrates drove the obese microbiota to a healthier profile, more similar to that of the lean microbiota.
34323161	10	34	with	microbiota	1608:1617	arg1	diversity					1636:1644	diversity	1636:1644	diversity	1636:1644	Nevertheless, passion fruit peel fermentation resulted in a microbiota with the highest diversity and evenness, a positive trait regarding host health.
34323161	10	34	with	microbiota	1608:1617	arg1	trait					1671:1675	a positive trait	1660:1675	a positive trait regarding host health	1660:1697	Nevertheless, passion fruit peel fermentation resulted in a microbiota with the highest diversity and evenness, a positive trait regarding host health.
34323161	10	34	with	microbiota	1608:1617	arg1	evenness					1650:1657	evenness	1650:1657	evenness	1650:1657	Nevertheless, passion fruit peel fermentation resulted in a microbiota with the highest diversity and evenness, a positive trait regarding host health.
34323161	1	35	dep	in	261:262	arg1	vitro					264:268	vitro	264:268	vitro	264:268	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	3	36	theme	gut	559:561	arg1	microbiota					563:572	the gut microbiota	555:572	the gut microbiota	555:572	The effects of the by-products on the dynamics of the gut microbiota differed according to the type of microbiota, as well as the type of by-product used.
34323161	1	37	dep	food-by-products	160:175	arg1	food-by-products					160:175	food-by-products	160:175	food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon	160:304	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	1	37	dep	food-by-products	160:175	arg1	bagasse					208:214	orange bagasse	201:214	orange bagasse (n=2)	201:220	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	1	37	dep	food-by-products	160:175	arg1	peel					240:243	passion fruit peel	226:243	passion fruit peel (n=3)	226:249	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	1	37	dep	food-by-products	160:175	arg1	bagasse					186:192	cassava bagasse	178:192	cassava bagasse (n=3)	178:198	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	1	37	dep	food-by-products	160:175	arg1	n=3					195:197	n=3	195:197	n=3	195:197	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	1	37	dep	food-by-products	160:175	arg1	n=2					217:219	n=2	217:219	n=2	217:219	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	1	37	dep	food-by-products	160:175	arg1	n=3					246:248	n=3	246:248	n=3	246:248	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	11	38	theme	valuable	1814:1821	arg1	material					1828:1835	valuable food material	1814:1835	valuable food material	1814:1835	In conclusion, the use of food-by-products could be an important step to tackle obesity and decrease the waste of valuable food material and consequently environmental pollution.
34323161	9	39	theme	intermediate	1492:1503	arg1	metabolites					1505:1515	intermediate metabolites	1492:1515	intermediate metabolites	1492:1515	Passion fruit peels were poorly fermented and generated negligible amounts of intermediate metabolites, indicating slow fermentation.
34323161	4	40	theme	carbohydrate	903:914	arg1	composition					916:926	primary carbohydrate composition	895:926	primary carbohydrate composition of the by-products (starch, fructan and pectin)	895:974	Principal Coordinate Analysis of the microbiota showed evidence of a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation, and instead, distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin) were present.
34323161	11	41	theme	important	1755:1763	arg1	step					1765:1768	an important step	1752:1768	an important step to tackle obesity and decrease the waste of valuable food material and consequently environmental pollution	1752:1876	In conclusion, the use of food-by-products could be an important step to tackle obesity and decrease the waste of valuable food material and consequently environmental pollution.
34323161	11	41	theme	important	1755:1763	arg1	use					1719:1721	the use	1715:1721	the use of food-by-products	1715:1741	In conclusion, the use of food-by-products could be an important step to tackle obesity and decrease the waste of valuable food material and consequently environmental pollution.
34323161	5	42	theme	obese	1037:1041	arg1	microbiota					1043:1052	the obese microbiota	1033:1052	the obese microbiota	1033:1052	This is evidence that the substrates drove the obese microbiota to a healthier profile, more similar to that of the lean microbiota.
34323161	12	43	theme	obese	2036:2040	arg1	microbiota					2042:2051	the obese microbiota	2032:2051	the obese microbiota	2032:2051	They are an inexpensive and non-invasive way to be used as a dietary intervention to improve health, as they were shown here to drive the composition of the obese microbiota to a healthier profile.
34323161	9	44	theme	slow	1529:1532	arg1	fermentation					1534:1545	slow fermentation	1529:1545	slow fermentation	1529:1545	Passion fruit peels were poorly fermented and generated negligible amounts of intermediate metabolites, indicating slow fermentation.
34323161	11	45	theme	food	1823:1826	arg1	material					1828:1835	valuable food material	1814:1835	valuable food material	1814:1835	In conclusion, the use of food-by-products could be an important step to tackle obesity and decrease the waste of valuable food material and consequently environmental pollution.
34323161	4	46	theme	microbiota	697:706	arg1	Analysis					681:688	Principal Coordinate Analysis	660:688	Principal Coordinate Analysis of the microbiota	660:706	Principal Coordinate Analysis of the microbiota showed evidence of a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation, and instead, distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin) were present.
34323161	1	47	theme	orange	201:206	arg1	food-by-products					160:175	food-by-products	160:175	food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon	160:304	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	1	47	theme	orange	201:206	arg1	n=2					217:219	n=2	217:219	n=2	217:219	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	1	47	theme	orange	201:206	arg1	bagasse					208:214	orange bagasse	201:214	orange bagasse (n=2)	201:220	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	3	48	theme	microbiota	608:617	arg1	type					600:603	the type	596:603	the type	596:603	The effects of the by-products on the dynamics of the gut microbiota differed according to the type of microbiota, as well as the type of by-product used.
34323161	3	48	theme	microbiota	608:617	arg1	type					635:638	the type	631:638	the type of by-product used	631:657	The effects of the by-products on the dynamics of the gut microbiota differed according to the type of microbiota, as well as the type of by-product used.
34323161	6	49	theme	genus	1164:1168	arg1	Bifidobacterium					1170:1184	the beneficial genus Bifidobacterium	1149:1184	the beneficial genus Bifidobacterium	1149:1184	Cassava bagasses enriched the beneficial genus Bifidobacterium in the obese microbiota.
34323161	4	50	theme	lean	758:761	arg1	microbiota					773:782	lean and obese microbiota	758:782	lean and obese microbiota before the addition of substrates, which disappeared after fermentation	758:854	Principal Coordinate Analysis of the microbiota showed evidence of a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation, and instead, distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin) were present.
34323161	5	51	theme	similar	1083:1089	arg1	profile					1069:1075	a healthier profile	1057:1075	a healthier profile	1057:1075	This is evidence that the substrates drove the obese microbiota to a healthier profile, more similar to that of the lean microbiota.
34323161	4	52	theme	obese	767:771	arg1	microbiota					773:782	lean and obese microbiota	758:782	lean and obese microbiota before the addition of substrates, which disappeared after fermentation	758:854	Principal Coordinate Analysis of the microbiota showed evidence of a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation, and instead, distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin) were present.
34323161	9	53	theme	Passion	1414:1420	arg1	peels					1428:1432	Passion fruit peels	1414:1432	Passion fruit peels	1414:1432	Passion fruit peels were poorly fermented and generated negligible amounts of intermediate metabolites, indicating slow fermentation.
34323161	4	54	dep	by-products	935:945	arg1	pectin					968:973	pectin	968:973	pectin	968:973	Principal Coordinate Analysis of the microbiota showed evidence of a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation, and instead, distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin) were present.
34323161	4	54	dep	by-products	935:945	arg1	fructan					956:962	fructan	956:962	fructan	956:962	Principal Coordinate Analysis of the microbiota showed evidence of a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation, and instead, distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin) were present.
34323161	4	54	dep	by-products	935:945	arg1	starch					948:953	starch	948:953	starch	948:953	Principal Coordinate Analysis of the microbiota showed evidence of a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation, and instead, distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin) were present.
34323161	4	54	dep	by-products	935:945	arg1	by-products					935:945	the by-products	931:945	the by-products (starch, fructan and pectin)	931:974	Principal Coordinate Analysis of the microbiota showed evidence of a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation, and instead, distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin) were present.
34323161	4	55	theme	clear	729:733	arg1	clustering					744:753	a clear separate clustering	727:753	a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation	727:854	Principal Coordinate Analysis of the microbiota showed evidence of a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation, and instead, distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin) were present.
34323161	1	56	theme	lean	392:395	arg1	people					406:411	lean or obese people	392:411	lean or obese people	392:411	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	1	57	theme	food-by-products	160:175	arg1	effects					149:155	the potential prebiotic effects	125:155	the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon	125:304	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	4	58	theme	clustering	744:753	arg1	evidence					715:722	evidence	715:722	evidence of a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation	715:854	Principal Coordinate Analysis of the microbiota showed evidence of a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation, and instead, distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin) were present.
34323161	3	59	theme	by-product	643:652	arg1	type					600:603	the type	596:603	the type	596:603	The effects of the by-products on the dynamics of the gut microbiota differed according to the type of microbiota, as well as the type of by-product used.
34323161	3	59	theme	by-product	643:652	arg1	type					635:638	the type	631:638	the type of by-product used	631:657	The effects of the by-products on the dynamics of the gut microbiota differed according to the type of microbiota, as well as the type of by-product used.
34323161	1	60	theme	obese	400:404	arg1	people					406:411	lean or obese people	392:411	lean or obese people	392:411	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	10	61	theme	host	1687:1690	arg1	health					1692:1697	host health	1687:1697	host health	1687:1697	Nevertheless, passion fruit peel fermentation resulted in a microbiota with the highest diversity and evenness, a positive trait regarding host health.
34323161	1	62	theme	cassava	178:184	arg1	food-by-products					160:175	food-by-products	160:175	food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon	160:304	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	1	62	theme	cassava	178:184	arg1	bagasse					186:192	cassava bagasse	178:192	cassava bagasse (n=3)	178:198	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	1	62	theme	cassava	178:184	arg1	n=3					195:197	n=3	195:197	n=3	195:197	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	4	63	theme	substrates	807:816	arg1	addition					795:802	the addition	791:802	the addition of substrates, which disappeared after fermentation	791:854	Principal Coordinate Analysis of the microbiota showed evidence of a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation, and instead, distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin) were present.
34323161	9	64	theme	fruit	1422:1426	arg1	peels					1428:1432	Passion fruit peels	1414:1432	Passion fruit peels	1414:1432	Passion fruit peels were poorly fermented and generated negligible amounts of intermediate metabolites, indicating slow fermentation.
34323161	8	65	theme	Orange	1331:1336	arg1	bagasses					1338:1345	Orange bagasses	1331:1345	Orange bagasses	1331:1345	Orange bagasses stimulated the growth of the butyrate-producing genus Coprococcus.
34323161	6	66	theme	obese	1193:1197	arg1	microbiota					1199:1208	the obese microbiota	1189:1208	the obese microbiota	1189:1208	Cassava bagasses enriched the beneficial genus Bifidobacterium in the obese microbiota.
34323161	2	67	theme	prebiotic	487:495	arg1	inulin					497:502	the prebiotic inulin	483:502	the prebiotic inulin	483:502	Fermentation of the by-products was compared to a control medium and the prebiotic inulin.
34323161	7	68	dep	control	1304:1310	arg1	medium					1312:1317	medium	1312:1317	medium	1312:1317	The production of total SCFA by cassava bagasses by the obese microbiota was higher than for control medium and inulin.
34323161	7	69	theme	total	1229:1233	arg1	SCFA					1235:1238	total SCFA	1229:1238	total SCFA	1229:1238	The production of total SCFA by cassava bagasses by the obese microbiota was higher than for control medium and inulin.
34323161	12	70	theme	dietary	1940:1946	arg1	intervention					1948:1959	a dietary intervention	1938:1959	a dietary intervention to improve health	1938:1977	They are an inexpensive and non-invasive way to be used as a dietary intervention to improve health, as they were shown here to drive the composition of the obese microbiota to a healthier profile.
34323161	0	71	theme	food-by-products	67:82	arg1	fermentation					51:62	in vitro fermentation	42:62	in vitro fermentation of food-by-products	42:82	Lean and obese microbiota: differences in in vitro fermentation of food-by-products.
34323161	1	72	theme	in	261:262	arg1	model					270:274	an in vitro model	258:274	an in vitro model simulating the proximal colon	258:304	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	1	73	theme	potential	129:137	arg1	effects					149:155	the potential prebiotic effects	125:155	the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon	125:304	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	4	74	theme	distinct	870:877	arg1	clusters					879:886	distinct clusters	870:886	distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin)	870:974	Principal Coordinate Analysis of the microbiota showed evidence of a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation, and instead, distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin) were present.
34323161	8	75	theme	genus	1395:1399	arg1	Coprococcus					1401:1411	the butyrate-producing genus Coprococcus	1372:1411	the butyrate-producing genus Coprococcus	1372:1411	Orange bagasses stimulated the growth of the butyrate-producing genus Coprococcus.
34323161	2	76	theme	control	464:470	arg1	medium					472:477	a control medium	462:477	a control medium	462:477	Fermentation of the by-products was compared to a control medium and the prebiotic inulin.
34323161	0	77	from	differences	27:37	arg1	fermentation					51:62	in vitro fermentation	42:62	in vitro fermentation of food-by-products	42:82	Lean and obese microbiota: differences in in vitro fermentation of food-by-products.
34323161	4	78	theme	Principal	660:668	arg1	Analysis					681:688	Principal Coordinate Analysis	660:688	Principal Coordinate Analysis of the microbiota	660:706	Principal Coordinate Analysis of the microbiota showed evidence of a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation, and instead, distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin) were present.
34323161	10	79	theme	passion	1562:1568	arg1	fermentation					1581:1592	passion fruit peel fermentation	1562:1592	passion fruit peel fermentation	1562:1592	Nevertheless, passion fruit peel fermentation resulted in a microbiota with the highest diversity and evenness, a positive trait regarding host health.
34323161	1	80	theme	fruit	234:238	arg1	peel					240:243	passion fruit peel	226:243	passion fruit peel (n=3)	226:249	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	1	80	theme	fruit	234:238	arg1	food-by-products					160:175	food-by-products	160:175	food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon	160:304	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	1	80	theme	fruit	234:238	arg1	n=3					246:248	n=3	246:248	n=3	246:248	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	9	81	theme	negligible	1470:1479	arg1	metabolites					1505:1515	intermediate metabolites	1492:1515	intermediate metabolites	1492:1515	Passion fruit peels were poorly fermented and generated negligible amounts of intermediate metabolites, indicating slow fermentation.
34323161	9	81	theme	negligible	1470:1479	arg1	amounts					1481:1487	negligible amounts	1470:1487	negligible amounts of intermediate metabolites	1470:1515	Passion fruit peels were poorly fermented and generated negligible amounts of intermediate metabolites, indicating slow fermentation.
34323161	3	82	from	effects	509:515	arg1	dynamics					543:550	the dynamics	539:550	the dynamics of the gut microbiota	539:572	The effects of the by-products on the dynamics of the gut microbiota differed according to the type of microbiota, as well as the type of by-product used.
34323161	0	83	dep	in	42:43	arg1	vitro					45:49	vitro	45:49	vitro	45:49	Lean and obese microbiota: differences in in vitro fermentation of food-by-products.
34323161	1	84	from	people	406:411	arg1	microbiota					376:385	faecal microbiota	369:385	faecal microbiota from lean or obese people	369:411	The aim of the study was to investigate the potential prebiotic effects of food-by-products (cassava bagasse (n=3), orange bagasse (n=2) and passion fruit peel (n=3)) using an in vitro model simulating the proximal colon, and to assess possible differences in fermentation when using faecal microbiota from lean or obese people.
34323161	4	85	theme	due	888:890	arg1	clusters					879:886	distinct clusters	870:886	distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin)	870:974	Principal Coordinate Analysis of the microbiota showed evidence of a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation, and instead, distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin) were present.
34323161	10	86	theme	peel	1576:1579	arg1	fermentation					1581:1592	passion fruit peel fermentation	1562:1592	passion fruit peel fermentation	1562:1592	Nevertheless, passion fruit peel fermentation resulted in a microbiota with the highest diversity and evenness, a positive trait regarding host health.
34323161	0	87	theme	in	42:43	arg1	fermentation					51:62	in vitro fermentation	42:62	in vitro fermentation of food-by-products	42:82	Lean and obese microbiota: differences in in vitro fermentation of food-by-products.
34323161	9	88	theme	metabolites	1505:1515	arg1	metabolites					1505:1515	intermediate metabolites	1492:1515	intermediate metabolites	1492:1515	Passion fruit peels were poorly fermented and generated negligible amounts of intermediate metabolites, indicating slow fermentation.
34323161	9	88	theme	metabolites	1505:1515	arg1	amounts					1481:1487	negligible amounts	1470:1487	negligible amounts of intermediate metabolites	1470:1515	Passion fruit peels were poorly fermented and generated negligible amounts of intermediate metabolites, indicating slow fermentation.
34323161	4	89	theme	primary	895:901	arg1	composition					916:926	primary carbohydrate composition	895:926	primary carbohydrate composition of the by-products (starch, fructan and pectin)	895:974	Principal Coordinate Analysis of the microbiota showed evidence of a clear separate clustering of lean and obese microbiota before the addition of substrates, which disappeared after fermentation, and instead, distinct clusters due to primary carbohydrate composition of the by-products (starch, fructan and pectin) were present.
34323161	0	90	dep	microbiota	15:24	arg1	differences					27:37	differences	27:37	Lean and obese microbiota: differences in in vitro fermentation of food-by-products.	0:83	Lean and obese microbiota: differences in in vitro fermentation of food-by-products.
34323161	3	91	theme	microbiota	563:572	arg1	dynamics					543:550	the dynamics	539:550	the dynamics of the gut microbiota	539:572	The effects of the by-products on the dynamics of the gut microbiota differed according to the type of microbiota, as well as the type of by-product used.
34323161	12	92	theme	microbiota	2042:2051	arg1	composition					2017:2027	the composition	2013:2027	the composition of the obese microbiota to a healthier profile	2013:2074	They are an inexpensive and non-invasive way to be used as a dietary intervention to improve health, as they were shown here to drive the composition of the obese microbiota to a healthier profile.
34323161	5	93	theme	lean	1106:1109	arg1	microbiota					1111:1120	the lean microbiota	1102:1120	the lean microbiota	1102:1120	This is evidence that the substrates drove the obese microbiota to a healthier profile, more similar to that of the lean microbiota.
32286801	8	0	from	encoding	1671:1678	arg1	patterns					1718:1725	patterns	1718:1725	patterns	1718:1725	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	8	0	from	encoding	1671:1678	arg1	structures					1703:1712	tailored structures	1694:1712	tailored structures	1694:1712	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	8	0	from	encoding	1671:1678	arg1	processes					1757:1765	programmed mineralization processes	1731:1765	programmed mineralization processes	1731:1765	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	8	1	theme	programmed	1731:1740	arg1	processes					1757:1765	programmed mineralization processes	1731:1765	programmed mineralization processes	1731:1765	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	3	2	theme	dense	539:543	arg1	mineralization					584:597	the dense, homogeneous, and spatially controlled mineralization	535:597	the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design	535:852	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	8	3	theme	CNF	1819:1821	arg1	biomaterials					1823:1834	mineralized CNF biomaterials	1807:1834	mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration	1807:1902	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	5	4	theme	mechanical	1276:1285	arg1	properties					1287:1296	wet state mechanical properties	1266:1296	wet state mechanical properties	1266:1296	The bone-inspired CNF bulk films exhibit a significantly increased stiffness, maintain good flexibility and translucency, and have a significant gain in wet state mechanical properties.
32286801	4	5	theme	mineral	958:964	arg1	contents					966:973	very high mineral contents	948:973	very high mineral contents	948:973	Our study reveals that anionic CNFs lead to a more homogeneous nanoscale mineralization with very high mineral contents up to ca. 70 wt % with a transition from amorphous to crystalline deposits, while cationic ChNFs yield rod-like crystalline morphologies.
32286801	7	6	theme	dialysis	1514:1521	arg1	reactor					1523:1529	a dialysis reactor	1512:1529	a dialysis reactor	1512:1529	Moreover, we also show a spatial control of the mineralization using kinetically controlled substrate uptake in a dialysis reactor, and by spatially selectively incorporating the enzyme into 2D printed filament patterns.
32286801	3	7	theme	nanoscale	778:786	arg1	blocks					797:802	biobased nanoscale building blocks	769:802	biobased nanoscale building blocks for sustainable high-performance materials design	769:852	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	3	8	theme	controlled	573:582	arg1	mineralization					584:597	the dense, homogeneous, and spatially controlled mineralization	535:597	the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design	535:852	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	4	9	theme	homogeneous	906:916	arg1	mineralization					928:941	a more homogeneous nanoscale mineralization	899:941	a more homogeneous nanoscale mineralization with very high mineral contents	899:973	Our study reveals that anionic CNFs lead to a more homogeneous nanoscale mineralization with very high mineral contents up to ca. 70 wt % with a transition from amorphous to crystalline deposits, while cationic ChNFs yield rod-like crystalline morphologies.
32286801	5	10	theme	state	1270:1274	arg1	properties					1287:1296	wet state mechanical properties	1266:1296	wet state mechanical properties	1266:1296	The bone-inspired CNF bulk films exhibit a significantly increased stiffness, maintain good flexibility and translucency, and have a significant gain in wet state mechanical properties.
32286801	2	11	theme	bioinspired	289:299	arg1	structures					301:310	even simplistic bioinspired structures	273:310	even simplistic bioinspired structures	273:310	The mimicry of even simplistic bioinspired structures, i.e., of densely and homogeneously mineralized nanofibrillar materials with controllable mechanical performance, continues to be a grand challenge.
32286801	7	12	theme	substrate	1492:1500	arg1	uptake					1502:1507	kinetically controlled substrate uptake	1469:1507	kinetically controlled substrate uptake	1469:1507	Moreover, we also show a spatial control of the mineralization using kinetically controlled substrate uptake in a dialysis reactor, and by spatially selectively incorporating the enzyme into 2D printed filament patterns.
32286801	3	13	theme	sustainable	808:818	arg1	design					847:852	sustainable high-performance materials design	808:852	sustainable high-performance materials design	808:852	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	5	14	from	gain	1258:1261	arg1	properties					1287:1296	wet state mechanical properties	1266:1296	wet state mechanical properties	1266:1296	The bone-inspired CNF bulk films exhibit a significantly increased stiffness, maintain good flexibility and translucency, and have a significant gain in wet state mechanical properties.
32286801	3	15	theme	phosphate	610:618	arg1	nanostructures					620:633	calcium phosphate nanostructures	602:633	calcium phosphate nanostructures	602:633	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	4	16	theme	wt	988:989	arg1	%					991:991	ca. 70 wt %	981:991	ca. 70 wt %	981:991	Our study reveals that anionic CNFs lead to a more homogeneous nanoscale mineralization with very high mineral contents up to ca. 70 wt % with a transition from amorphous to crystalline deposits, while cationic ChNFs yield rod-like crystalline morphologies.
32286801	1	17	theme	mineralized	213:223	arg1	materials					247:255	densely mineralized and ordered nanofiber materials	205:255	densely mineralized and ordered nanofiber materials	205:255	Many biological high-performance composites, such as bone, antler, and crustacean cuticles, are composed of densely mineralized and ordered nanofiber materials.
32286801	7	18	theme	mineralization	1448:1461	arg1	control					1433:1439	a spatial control	1423:1439	a spatial control of the mineralization using kinetically controlled substrate uptake in a dialysis reactor	1423:1529	Moreover, we also show a spatial control of the mineralization using kinetically controlled substrate uptake in a dialysis reactor, and by spatially selectively incorporating the enzyme into 2D printed filament patterns.
32286801	1	19	theme	ordered	229:235	arg1	materials					247:255	densely mineralized and ordered nanofiber materials	205:255	densely mineralized and ordered nanofiber materials	205:255	Many biological high-performance composites, such as bone, antler, and crustacean cuticles, are composed of densely mineralized and ordered nanofiber materials.
32286801	6	20	theme	mechanical	1303:1312	arg1	properties					1314:1323	The mechanical properties	1299:1323	The mechanical properties	1299:1323	The mechanical properties can be tuned both by the enzyme concentration and the mineralization time.
32286801	3	21	theme	materials	837:845	arg1	design					847:852	sustainable high-performance materials design	808:852	sustainable high-performance materials design	808:852	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	2	22	theme	simplistic	278:287	arg1	structures					301:310	even simplistic bioinspired structures	273:310	even simplistic bioinspired structures	273:310	The mimicry of even simplistic bioinspired structures, i.e., of densely and homogeneously mineralized nanofibrillar materials with controllable mechanical performance, continues to be a grand challenge.
32286801	7	23	theme	spatial	1425:1431	arg1	control					1433:1439	a spatial control	1423:1439	a spatial control of the mineralization using kinetically controlled substrate uptake in a dialysis reactor	1423:1529	Moreover, we also show a spatial control of the mineralization using kinetically controlled substrate uptake in a dialysis reactor, and by spatially selectively incorporating the enzyme into 2D printed filament patterns.
32286801	5	24	theme	CNF	1131:1133	arg1	films					1140:1144	The bone-inspired CNF bulk films	1113:1144	The bone-inspired CNF bulk films	1113:1144	The bone-inspired CNF bulk films exhibit a significantly increased stiffness, maintain good flexibility and translucency, and have a significant gain in wet state mechanical properties.
32286801	3	25	theme	nanofibrils	684:694	arg1	-both					754:758	anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both	654:758	anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design	654:852	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	4	26	theme	crystalline	1029:1039	arg1	deposits					1041:1048	crystalline deposits	1029:1048	crystalline deposits	1029:1048	Our study reveals that anionic CNFs lead to a more homogeneous nanoscale mineralization with very high mineral contents up to ca. 70 wt % with a transition from amorphous to crystalline deposits, while cationic ChNFs yield rod-like crystalline morphologies.
32286801	5	27	theme	good	1200:1203	arg1	flexibility					1205:1215	good flexibility	1200:1215	good flexibility	1200:1215	The bone-inspired CNF bulk films exhibit a significantly increased stiffness, maintain good flexibility and translucency, and have a significant gain in wet state mechanical properties.
32286801	3	28	theme	charged	666:672	arg1	CNFs					697:700	CNFs	697:700	CNFs	697:700	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	3	28	theme	charged	666:672	arg1	nanofibrils					684:694	anionically charged cellulose nanofibrils	654:694	anionically charged cellulose nanofibrils (CNFs)	654:701	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	4	29	with	mineralization	928:941	arg1	contents					966:973	very high mineral contents	948:973	very high mineral contents	948:973	Our study reveals that anionic CNFs lead to a more homogeneous nanoscale mineralization with very high mineral contents up to ca. 70 wt % with a transition from amorphous to crystalline deposits, while cationic ChNFs yield rod-like crystalline morphologies.
32286801	5	30	theme	increased	1170:1178	arg1	stiffness					1180:1188	a significantly increased stiffness	1154:1188	a significantly increased stiffness	1154:1188	The bone-inspired CNF bulk films exhibit a significantly increased stiffness, maintain good flexibility and translucency, and have a significant gain in wet state mechanical properties.
32286801	4	31	dep	70	985:986	arg1	ca.					981:983	ca.	981:983	ca.	981:983	Our study reveals that anionic CNFs lead to a more homogeneous nanoscale mineralization with very high mineral contents up to ca. 70 wt % with a transition from amorphous to crystalline deposits, while cationic ChNFs yield rod-like crystalline morphologies.
32286801	1	32	theme	biological	102:111	arg1	composites					130:139	Many biological high-performance composites	97:139	Many biological high-performance composites	97:139	Many biological high-performance composites, such as bone, antler, and crustacean cuticles, are composed of densely mineralized and ordered nanofiber materials.
32286801	1	32	theme	biological	102:111	arg1	antler					156:161	antler	156:161	antler	156:161	Many biological high-performance composites, such as bone, antler, and crustacean cuticles, are composed of densely mineralized and ordered nanofiber materials.
32286801	1	32	theme	biological	102:111	arg1	bone					150:153	bone	150:153	bone	150:153	Many biological high-performance composites, such as bone, antler, and crustacean cuticles, are composed of densely mineralized and ordered nanofiber materials.
32286801	1	32	theme	biological	102:111	arg1	cuticles					179:186	crustacean cuticles	168:186	crustacean cuticles	168:186	Many biological high-performance composites, such as bone, antler, and crustacean cuticles, are composed of densely mineralized and ordered nanofiber materials.
32286801	4	33	theme	crystalline	1087:1097	arg1	morphologies					1099:1110	rod-like crystalline morphologies	1078:1110	rod-like crystalline morphologies	1078:1110	Our study reveals that anionic CNFs lead to a more homogeneous nanoscale mineralization with very high mineral contents up to ca. 70 wt % with a transition from amorphous to crystalline deposits, while cationic ChNFs yield rod-like crystalline morphologies.
32286801	5	34	theme	significant	1246:1256	arg1	gain					1258:1261	a significant gain	1244:1261	a significant gain in wet state mechanical properties	1244:1296	The bone-inspired CNF bulk films exhibit a significantly increased stiffness, maintain good flexibility and translucency, and have a significant gain in wet state mechanical properties.
32286801	0	35	theme	Biomimetic	19:28	arg1	Materials					55:63	Highly Mineralized Biomimetic Polysaccharide Nanofiber Materials	0:63	Highly Mineralized Biomimetic Polysaccharide Nanofiber Materials	0:63	Highly Mineralized Biomimetic Polysaccharide Nanofiber Materials Using Enzymatic Mineralization.
32286801	4	36	theme	cationic	1057:1064	arg1	ChNFs					1066:1070	cationic ChNFs	1057:1070	cationic ChNFs	1057:1070	Our study reveals that anionic CNFs lead to a more homogeneous nanoscale mineralization with very high mineral contents up to ca. 70 wt % with a transition from amorphous to crystalline deposits, while cationic ChNFs yield rod-like crystalline morphologies.
32286801	2	37	theme	grand	444:448	arg1	challenge					450:458	a grand challenge	442:458	a grand challenge	442:458	The mimicry of even simplistic bioinspired structures, i.e., of densely and homogeneously mineralized nanofibrillar materials with controllable mechanical performance, continues to be a grand challenge.
32286801	2	37	theme	grand	444:448	arg1	mimicry					262:268	The mimicry	258:268	The mimicry	258:268	The mimicry of even simplistic bioinspired structures, i.e., of densely and homogeneously mineralized nanofibrillar materials with controllable mechanical performance, continues to be a grand challenge.
32286801	0	38	theme	Nanofiber	45:53	arg1	Materials					55:63	Highly Mineralized Biomimetic Polysaccharide Nanofiber Materials	0:63	Highly Mineralized Biomimetic Polysaccharide Nanofiber Materials	0:63	Highly Mineralized Biomimetic Polysaccharide Nanofiber Materials Using Enzymatic Mineralization.
32286801	2	39	with	materials	374:382	arg1	performance					413:423	controllable mechanical performance	389:423	controllable mechanical performance	389:423	The mimicry of even simplistic bioinspired structures, i.e., of densely and homogeneously mineralized nanofibrillar materials with controllable mechanical performance, continues to be a grand challenge.
32286801	2	40	theme	materials	374:382	arg1	challenge					450:458	a grand challenge	442:458	a grand challenge	442:458	The mimicry of even simplistic bioinspired structures, i.e., of densely and homogeneously mineralized nanofibrillar materials with controllable mechanical performance, continues to be a grand challenge.
32286801	2	40	theme	materials	374:382	arg1	mimicry					262:268	The mimicry	258:268	The mimicry	258:268	The mimicry of even simplistic bioinspired structures, i.e., of densely and homogeneously mineralized nanofibrillar materials with controllable mechanical performance, continues to be a grand challenge.
32286801	7	41	theme	printed	1594:1600	arg1	patterns					1611:1618	2D printed filament patterns	1591:1618	2D printed filament patterns	1591:1618	Moreover, we also show a spatial control of the mineralization using kinetically controlled substrate uptake in a dialysis reactor, and by spatially selectively incorporating the enzyme into 2D printed filament patterns.
32286801	8	42	from	enzymes	1683:1689	arg1	patterns					1718:1725	patterns	1718:1725	patterns	1718:1725	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	8	42	from	enzymes	1683:1689	arg1	structures					1703:1712	tailored structures	1694:1712	tailored structures	1694:1712	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	8	42	from	enzymes	1683:1689	arg1	processes					1757:1765	programmed mineralization processes	1731:1765	programmed mineralization processes	1731:1765	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	8	43	theme	enzymes	1683:1689	arg1	encoding					1671:1678	spatial encoding	1663:1678	spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes	1663:1765	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	3	44	theme	nanofibrils	735:745	arg1	-both					754:758	anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both	654:758	anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design	654:852	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	2	45	theme	mineralized	348:358	arg1	materials					374:382	densely and homogeneously mineralized nanofibrillar materials	322:382	densely and homogeneously mineralized nanofibrillar materials with controllable mechanical performance	322:423	The mimicry of even simplistic bioinspired structures, i.e., of densely and homogeneously mineralized nanofibrillar materials with controllable mechanical performance, continues to be a grand challenge.
32286801	1	46	theme	nanofiber	237:245	arg1	materials					247:255	densely mineralized and ordered nanofiber materials	205:255	densely mineralized and ordered nanofiber materials	205:255	Many biological high-performance composites, such as bone, antler, and crustacean cuticles, are composed of densely mineralized and ordered nanofiber materials.
32286801	8	47	theme	tailored	1694:1701	arg1	structures					1703:1712	tailored structures	1694:1712	tailored structures	1694:1712	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	3	48	theme	charged	720:726	arg1	ChNFs					748:752	ChNFs	748:752	ChNFs	748:752	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	3	48	theme	charged	720:726	arg1	nanofibrils					735:745	cationically charged chitin nanofibrils	707:745	cationically charged chitin nanofibrils (ChNFs)	707:753	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	4	49	theme	anionic	878:884	arg1	CNFs					886:889	anionic CNFs	878:889	anionic CNFs	878:889	Our study reveals that anionic CNFs lead to a more homogeneous nanoscale mineralization with very high mineral contents up to ca. 70 wt % with a transition from amorphous to crystalline deposits, while cationic ChNFs yield rod-like crystalline morphologies.
32286801	3	50	theme	-both	754:758	arg1	networks					642:649	networks	642:649	networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design	642:852	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	2	51	theme	controllable	389:400	arg1	performance					413:423	controllable mechanical performance	389:423	controllable mechanical performance	389:423	The mimicry of even simplistic bioinspired structures, i.e., of densely and homogeneously mineralized nanofibrillar materials with controllable mechanical performance, continues to be a grand challenge.
32286801	4	52	from	amorphous	1016:1024	arg1	transition					1000:1009	a transition	998:1009	a transition from amorphous to crystalline deposits	998:1048	Our study reveals that anionic CNFs lead to a more homogeneous nanoscale mineralization with very high mineral contents up to ca. 70 wt % with a transition from amorphous to crystalline deposits, while cationic ChNFs yield rod-like crystalline morphologies.
32286801	6	53	theme	enzyme	1350:1355	arg1	concentration					1357:1369	the enzyme concentration	1346:1369	the enzyme concentration	1346:1369	The mechanical properties can be tuned both by the enzyme concentration and the mineralization time.
32286801	8	54	theme	mineralized	1807:1817	arg1	biomaterials					1823:1834	mineralized CNF biomaterials	1807:1834	mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration	1807:1902	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	4	55	theme	high	953:956	arg1	contents					966:973	very high mineral contents	948:973	very high mineral contents	948:973	Our study reveals that anionic CNFs lead to a more homogeneous nanoscale mineralization with very high mineral contents up to ca. 70 wt % with a transition from amorphous to crystalline deposits, while cationic ChNFs yield rod-like crystalline morphologies.
32286801	8	56	theme	mineralization	1742:1755	arg1	processes					1757:1765	programmed mineralization processes	1731:1765	programmed mineralization processes	1731:1765	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	3	57	theme	building	788:795	arg1	blocks					797:802	biobased nanoscale building blocks	769:802	biobased nanoscale building blocks for sustainable high-performance materials design	769:852	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	2	58	theme	structures	301:310	arg1	challenge					450:458	a grand challenge	442:458	a grand challenge	442:458	The mimicry of even simplistic bioinspired structures, i.e., of densely and homogeneously mineralized nanofibrillar materials with controllable mechanical performance, continues to be a grand challenge.
32286801	2	58	theme	structures	301:310	arg1	mimicry					262:268	The mimicry	258:268	The mimicry	258:268	The mimicry of even simplistic bioinspired structures, i.e., of densely and homogeneously mineralized nanofibrillar materials with controllable mechanical performance, continues to be a grand challenge.
32286801	8	59	theme	biomaterials	1823:1834	arg1	application					1792:1802	the potential application	1778:1802	the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general	1778:1913	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	6	60	dep	concentration	1357:1369	arg1	both					1338:1341	both	1338:1341	both	1338:1341	The mechanical properties can be tuned both by the enzyme concentration and the mineralization time.
32286801	3	61	theme	biobased	769:776	arg1	blocks					797:802	biobased nanoscale building blocks	769:802	biobased nanoscale building blocks for sustainable high-performance materials design	769:852	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	3	62	theme	calcium	602:608	arg1	nanostructures					620:633	calcium phosphate nanostructures	602:633	calcium phosphate nanostructures	602:633	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	8	63	theme	complex	1841:1847	arg1	gradients					1849:1857	complex gradients	1841:1857	complex gradients for bone substitutes and tissue regeneration	1841:1902	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	4	64	theme	nanoscale	918:926	arg1	mineralization					928:941	a more homogeneous nanoscale mineralization	899:941	a more homogeneous nanoscale mineralization with very high mineral contents	899:973	Our study reveals that anionic CNFs lead to a more homogeneous nanoscale mineralization with very high mineral contents up to ca. 70 wt % with a transition from amorphous to crystalline deposits, while cationic ChNFs yield rod-like crystalline morphologies.
32286801	3	65	theme	high-performance	820:835	arg1	design					847:852	sustainable high-performance materials design	808:852	sustainable high-performance materials design	808:852	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	8	66	from	patterns	1718:1725	arg1	encoding					1671:1678	spatial encoding	1663:1678	spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes	1663:1765	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	7	67	theme	controlled	1481:1490	arg1	uptake					1502:1507	kinetically controlled substrate uptake	1469:1507	kinetically controlled substrate uptake	1469:1507	Moreover, we also show a spatial control of the mineralization using kinetically controlled substrate uptake in a dialysis reactor, and by spatially selectively incorporating the enzyme into 2D printed filament patterns.
32286801	8	68	from	structures	1703:1712	arg1	encoding					1671:1678	spatial encoding	1663:1678	spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes	1663:1765	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	8	69	from	processes	1757:1765	arg1	encoding					1671:1678	spatial encoding	1663:1678	spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes	1663:1765	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	3	70	theme	nanostructures	620:633	arg1	mineralization					584:597	the dense, homogeneous, and spatially controlled mineralization	535:597	the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design	535:852	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	8	71	theme	tissue	1884:1889	arg1	regeneration					1891:1902	tissue regeneration	1884:1902	tissue regeneration	1884:1902	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	5	72	contain	have	1239:1242	arg2	gain					1258:1261	a significant gain	1244:1261	a significant gain in wet state mechanical properties	1244:1296	The bone-inspired CNF bulk films exhibit a significantly increased stiffness, maintain good flexibility and translucency, and have a significant gain in wet state mechanical properties.
32286801	5	72	contain	have	1239:1242	arg1	films					1140:1144	The bone-inspired CNF bulk films	1113:1144	The bone-inspired CNF bulk films	1113:1144	The bone-inspired CNF bulk films exhibit a significantly increased stiffness, maintain good flexibility and translucency, and have a significant gain in wet state mechanical properties.
32286801	6	73	theme	mineralization	1379:1392	arg1	time					1394:1397	the mineralization time	1375:1397	the mineralization time	1375:1397	The mechanical properties can be tuned both by the enzyme concentration and the mineralization time.
32286801	5	74	theme	bone-inspired	1117:1129	arg1	films					1140:1144	The bone-inspired CNF bulk films	1113:1144	The bone-inspired CNF bulk films	1113:1144	The bone-inspired CNF bulk films exhibit a significantly increased stiffness, maintain good flexibility and translucency, and have a significant gain in wet state mechanical properties.
32286801	3	75	theme	cellulose	674:682	arg1	CNFs					697:700	CNFs	697:700	CNFs	697:700	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	3	75	theme	cellulose	674:682	arg1	nanofibrils					684:694	anionically charged cellulose nanofibrils	654:694	anionically charged cellulose nanofibrils (CNFs)	654:701	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	5	76	theme	bulk	1135:1138	arg1	films					1140:1144	The bone-inspired CNF bulk films	1113:1144	The bone-inspired CNF bulk films	1113:1144	The bone-inspired CNF bulk films exhibit a significantly increased stiffness, maintain good flexibility and translucency, and have a significant gain in wet state mechanical properties.
32286801	1	77	theme	crustacean	168:177	arg1	cuticles					179:186	crustacean cuticles	168:186	crustacean cuticles	168:186	Many biological high-performance composites, such as bone, antler, and crustacean cuticles, are composed of densely mineralized and ordered nanofiber materials.
32286801	8	78	with	biomaterials	1823:1834	arg1	gradients					1849:1857	complex gradients	1841:1857	complex gradients for bone substitutes and tissue regeneration	1841:1902	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	8	79	dep	bone	1863:1866	arg1	substitutes					1868:1878	substitutes	1868:1878	substitutes	1868:1878	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	1	80	theme	Many	97:100	arg1	composites					130:139	Many biological high-performance composites	97:139	Many biological high-performance composites	97:139	Many biological high-performance composites, such as bone, antler, and crustacean cuticles, are composed of densely mineralized and ordered nanofiber materials.
32286801	1	80	theme	Many	97:100	arg1	antler					156:161	antler	156:161	antler	156:161	Many biological high-performance composites, such as bone, antler, and crustacean cuticles, are composed of densely mineralized and ordered nanofiber materials.
32286801	1	80	theme	Many	97:100	arg1	bone					150:153	bone	150:153	bone	150:153	Many biological high-performance composites, such as bone, antler, and crustacean cuticles, are composed of densely mineralized and ordered nanofiber materials.
32286801	1	80	theme	Many	97:100	arg1	cuticles					179:186	crustacean cuticles	168:186	crustacean cuticles	168:186	Many biological high-performance composites, such as bone, antler, and crustacean cuticles, are composed of densely mineralized and ordered nanofiber materials.
32286801	3	81	theme	alkaline	473:480	arg1	catalyst					510:517	an enzymatic catalyst	497:517	an enzymatic catalyst	497:517	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	3	81	theme	alkaline	473:480	arg1	phosphatase					482:492	alkaline phosphatase	473:492	alkaline phosphatase	473:492	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	0	82	theme	Mineralized	7:17	arg1	Materials					55:63	Highly Mineralized Biomimetic Polysaccharide Nanofiber Materials	0:63	Highly Mineralized Biomimetic Polysaccharide Nanofiber Materials	0:63	Highly Mineralized Biomimetic Polysaccharide Nanofiber Materials Using Enzymatic Mineralization.
32286801	1	83	theme	high-performance	113:128	arg1	composites					130:139	Many biological high-performance composites	97:139	Many biological high-performance composites	97:139	Many biological high-performance composites, such as bone, antler, and crustacean cuticles, are composed of densely mineralized and ordered nanofiber materials.
32286801	1	83	theme	high-performance	113:128	arg1	antler					156:161	antler	156:161	antler	156:161	Many biological high-performance composites, such as bone, antler, and crustacean cuticles, are composed of densely mineralized and ordered nanofiber materials.
32286801	1	83	theme	high-performance	113:128	arg1	bone					150:153	bone	150:153	bone	150:153	Many biological high-performance composites, such as bone, antler, and crustacean cuticles, are composed of densely mineralized and ordered nanofiber materials.
32286801	1	83	theme	high-performance	113:128	arg1	cuticles					179:186	crustacean cuticles	168:186	crustacean cuticles	168:186	Many biological high-performance composites, such as bone, antler, and crustacean cuticles, are composed of densely mineralized and ordered nanofiber materials.
32286801	4	84	theme	rod-like	1078:1085	arg1	morphologies					1099:1110	rod-like crystalline morphologies	1078:1110	rod-like crystalline morphologies	1078:1110	Our study reveals that anionic CNFs lead to a more homogeneous nanoscale mineralization with very high mineral contents up to ca. 70 wt % with a transition from amorphous to crystalline deposits, while cationic ChNFs yield rod-like crystalline morphologies.
32286801	0	85	theme	Polysaccharide	30:43	arg1	Materials					55:63	Highly Mineralized Biomimetic Polysaccharide Nanofiber Materials	0:63	Highly Mineralized Biomimetic Polysaccharide Nanofiber Materials	0:63	Highly Mineralized Biomimetic Polysaccharide Nanofiber Materials Using Enzymatic Mineralization.
32286801	8	86	theme	spatial	1663:1669	arg1	encoding					1671:1678	spatial encoding	1663:1678	spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes	1663:1765	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	8	87	from	application	1792:1802	arg1	general					1907:1913	general	1907:1913	general	1907:1913	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	3	88	theme	enzymatic	500:508	arg1	catalyst					510:517	an enzymatic catalyst	497:517	an enzymatic catalyst	497:517	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	3	88	theme	enzymatic	500:508	arg1	phosphatase					482:492	alkaline phosphatase	473:492	alkaline phosphatase	473:492	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	5	89	theme	wet	1266:1268	arg1	properties					1287:1296	wet state mechanical properties	1266:1296	wet state mechanical properties	1266:1296	The bone-inspired CNF bulk films exhibit a significantly increased stiffness, maintain good flexibility and translucency, and have a significant gain in wet state mechanical properties.
32286801	0	90	theme	Enzymatic	71:79	arg1	Mineralization					81:94	Enzymatic Mineralization	71:94	Enzymatic Mineralization	71:94	Highly Mineralized Biomimetic Polysaccharide Nanofiber Materials Using Enzymatic Mineralization.
32286801	3	91	theme	chitin	728:733	arg1	ChNFs					748:752	ChNFs	748:752	ChNFs	748:752	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	3	91	theme	chitin	728:733	arg1	nanofibrils					735:745	cationically charged chitin nanofibrils	707:745	cationically charged chitin nanofibrils (ChNFs)	707:753	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32286801	2	92	theme	nanofibrillar	360:372	arg1	materials					374:382	densely and homogeneously mineralized nanofibrillar materials	322:382	densely and homogeneously mineralized nanofibrillar materials with controllable mechanical performance	322:423	The mimicry of even simplistic bioinspired structures, i.e., of densely and homogeneously mineralized nanofibrillar materials with controllable mechanical performance, continues to be a grand challenge.
32286801	7	93	theme	filament	1602:1609	arg1	patterns					1611:1618	2D printed filament patterns	1591:1618	2D printed filament patterns	1591:1618	Moreover, we also show a spatial control of the mineralization using kinetically controlled substrate uptake in a dialysis reactor, and by spatially selectively incorporating the enzyme into 2D printed filament patterns.
32286801	2	94	theme	mechanical	402:411	arg1	performance					413:423	controllable mechanical performance	389:423	controllable mechanical performance	389:423	The mimicry of even simplistic bioinspired structures, i.e., of densely and homogeneously mineralized nanofibrillar materials with controllable mechanical performance, continues to be a grand challenge.
32286801	8	95	theme	potential	1782:1790	arg1	application					1792:1802	the potential application	1778:1802	the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general	1778:1913	The strategy highlights possibilities for spatial encoding of enzymes in tailored structures and patterns and programmed mineralization processes, promoting the potential application of mineralized CNF biomaterials with complex gradients for bone substitutes and tissue regeneration in general.
32286801	7	96	theme	2D	1591:1592	arg1	patterns					1611:1618	2D printed filament patterns	1591:1618	2D printed filament patterns	1591:1618	Moreover, we also show a spatial control of the mineralization using kinetically controlled substrate uptake in a dialysis reactor, and by spatially selectively incorporating the enzyme into 2D printed filament patterns.
32286801	3	97	theme	homogeneous	546:556	arg1	mineralization					584:597	the dense, homogeneous, and spatially controlled mineralization	535:597	the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design	535:852	Here, using alkaline phosphatase as an enzymatic catalyst, we demonstrate the dense, homogeneous, and spatially controlled mineralization of calcium phosphate nanostructures within networks of anionically charged cellulose nanofibrils (CNFs) and cationically charged chitin nanofibrils (ChNFs)-both emerging biobased nanoscale building blocks for sustainable high-performance materials design.
32577715	8	0	theme	lubricin	1475:1482	arg1	concentrations					1457:1470	low concentrations	1453:1470	low concentrations of lubricin	1453:1482	Interestingly, this aberrant behavior was not eliminated by the addition of HA, and it was associated with low concentrations of lubricin.
32577715	7	1	theme	variable	1303:1310	arg1	coefficients					1332:1343	highly variable and larger friction coefficients	1296:1343	highly variable and larger friction coefficients	1296:1343	While some of the IA SF also showed typical Stribeck behavior, a subset showed more erratic behavior with highly variable and larger friction coefficients.
32577715	3	2	theme	study	484:488	arg1	objective					466:474	The objective	462:474	The objective of this study	462:488	The objective of this study was to investigate the effects of the HA viscosupplement, Hymovis®, on the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA).
32577715	3	3	from	effects	513:519	arg1	properties					577:586	the lubricating properties	561:586	the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA)	561:671	The objective of this study was to investigate the effects of the HA viscosupplement, Hymovis®, on the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA).
32577715	1	4	theme	OA	237:238	arg1	patients					240:247	OA patients	237:247	OA patients	237:247	Inferior synovial lubrication is a hallmark of osteoarthritis (OA), and synovial fluid (SF) lubrication and composition are variable among OA patients.
32577715	9	5	theme	typical	1595:1601	arg1	behavior					1616:1623	typical tribological behavior	1595:1623	typical tribological behavior	1595:1623	Aberrant SF exhibited significantly lower effective viscosities compared to noninflammatory OA and IA SF with typical tribological behavior.
32577715	6	6	theme	HA	1186:1187	arg1	addition					1174:1181	the addition	1170:1181	the addition of HA	1170:1187	In noninflammatory OA SF, friction coefficients followed a typical Stribeck pattern, and their magnitude was decreased by the addition of HA.
32577715	2	7	theme	SF	331:332	arg1	viscoelasticity					334:348	SF viscoelasticity	331:348	SF viscoelasticity	331:348	Hyaluronic acid (HA) viscosupplementation is a widely used therapy for improving SF viscoelasticity and lubrication, but it is unclear how the effectiveness of HA viscosupplements varies with arthritic endotype.
32577715	6	8	theme	Stribeck	1115:1122	arg1	pattern					1124:1130	a typical Stribeck pattern	1105:1130	a typical Stribeck pattern	1105:1130	In noninflammatory OA SF, friction coefficients followed a typical Stribeck pattern, and their magnitude was decreased by the addition of HA.
32577715	7	9	theme	larger	1316:1321	arg1	coefficients					1332:1343	highly variable and larger friction coefficients	1296:1343	highly variable and larger friction coefficients	1296:1343	While some of the IA SF also showed typical Stribeck behavior, a subset showed more erratic behavior with highly variable and larger friction coefficients.
32577715	7	10	theme	erratic	1274:1280	arg1	behavior					1282:1289	more erratic behavior	1269:1289	more erratic behavior	1269:1289	While some of the IA SF also showed typical Stribeck behavior, a subset showed more erratic behavior with highly variable and larger friction coefficients.
32577715	3	11	from	patients	608:615	arg1	properties					577:586	the lubricating properties	561:586	the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA)	561:671	The objective of this study was to investigate the effects of the HA viscosupplement, Hymovis®, on the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA).
32577715	3	11	from	patients	608:615	arg1	SF					600:601	diseased SF	591:601	diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA)	591:671	The objective of this study was to investigate the effects of the HA viscosupplement, Hymovis®, on the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA).
32577715	5	12	theme	HA	996:997	arg1	addition					984:991	the addition	980:991	the addition of HA	980:997	Using rotational rheometry, no difference in SF viscosity was detected between disease types, and the addition of HA significantly increased all fluids' viscosities.
32577715	4	13	theme	lubricin	709:716	arg1	concentrations					718:731	lubricin concentrations	709:731	lubricin concentrations	709:731	The composition (cytokine, HA, and lubricin concentrations) of the SF was measured as well as the mechanical properties (rheology, tribology) of the SF alone and in a 1:1 mixture with the HA viscosupplement.
32577715	0	14	theme	Inflammatory	0:11	arg1	Fluids					42:47	Inflammatory and Noninflammatory Synovial Fluids	0:47	Inflammatory and Noninflammatory Synovial Fluids	0:47	Inflammatory and Noninflammatory Synovial Fluids Exhibit New and Distinct Tribological Endotypes.
32577715	3	15	from	properties	577:586	arg1	patients					608:615	patients	608:615	patients with noninflammatory OA and inflammatory arthritis (IA)	608:671	The objective of this study was to investigate the effects of the HA viscosupplement, Hymovis®, on the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA).
32577715	1	16	theme	osteoarthritis	145:158	arg1	lubrication					116:126	Inferior synovial lubrication	98:126	Inferior synovial lubrication	98:126	Inferior synovial lubrication is a hallmark of osteoarthritis (OA), and synovial fluid (SF) lubrication and composition are variable among OA patients.
32577715	1	16	theme	osteoarthritis	145:158	arg1	hallmark					133:140	a hallmark	131:140	a hallmark of osteoarthritis (OA)	131:163	Inferior synovial lubrication is a hallmark of osteoarthritis (OA), and synovial fluid (SF) lubrication and composition are variable among OA patients.
32577715	4	17	with	mixture	845:851	arg1	viscosupplement					865:879	the HA viscosupplement	858:879	the HA viscosupplement	858:879	The composition (cytokine, HA, and lubricin concentrations) of the SF was measured as well as the mechanical properties (rheology, tribology) of the SF alone and in a 1:1 mixture with the HA viscosupplement.
32577715	8	18	theme	aberrant	1366:1373	arg1	behavior					1375:1382	this aberrant behavior	1361:1382	this aberrant behavior	1361:1382	Interestingly, this aberrant behavior was not eliminated by the addition of HA, and it was associated with low concentrations of lubricin.
32577715	5	19	theme	rotational	888:897	arg1	rheometry					899:907	rotational rheometry	888:907	rotational rheometry	888:907	Using rotational rheometry, no difference in SF viscosity was detected between disease types, and the addition of HA significantly increased all fluids' viscosities.
32577715	7	20	theme	IA	1208:1209	arg1	SF					1211:1212	the IA SF	1204:1212	the IA SF	1204:1212	While some of the IA SF also showed typical Stribeck behavior, a subset showed more erratic behavior with highly variable and larger friction coefficients.
32577715	4	21	theme	HA	862:863	arg1	viscosupplement					865:879	the HA viscosupplement	858:879	the HA viscosupplement	858:879	The composition (cytokine, HA, and lubricin concentrations) of the SF was measured as well as the mechanical properties (rheology, tribology) of the SF alone and in a 1:1 mixture with the HA viscosupplement.
32577715	4	22	theme	mechanical	772:781	arg1	composition					678:688	The composition	674:688	The composition (cytokine, HA, and lubricin concentrations) of the SF	674:742	The composition (cytokine, HA, and lubricin concentrations) of the SF was measured as well as the mechanical properties (rheology, tribology) of the SF alone and in a 1:1 mixture with the HA viscosupplement.
32577715	4	22	theme	mechanical	772:781	arg1	rheology					795:802	rheology	795:802	rheology	795:802	The composition (cytokine, HA, and lubricin concentrations) of the SF was measured as well as the mechanical properties (rheology, tribology) of the SF alone and in a 1:1 mixture with the HA viscosupplement.
32577715	4	22	theme	mechanical	772:781	arg1	properties					783:792	the mechanical properties	768:792	the mechanical properties (rheology, tribology) of the SF alone	768:830	The composition (cytokine, HA, and lubricin concentrations) of the SF was measured as well as the mechanical properties (rheology, tribology) of the SF alone and in a 1:1 mixture with the HA viscosupplement.
32577715	6	23	theme	noninflammatory	1051:1065	arg1	SF					1070:1071	noninflammatory OA SF	1051:1071	noninflammatory OA SF	1051:1071	In noninflammatory OA SF, friction coefficients followed a typical Stribeck pattern, and their magnitude was decreased by the addition of HA.
32577715	2	24	theme	acid	261:264	arg1	therapy					309:315	a widely used therapy	295:315	a widely used therapy for improving SF viscoelasticity and lubrication	295:364	Hyaluronic acid (HA) viscosupplementation is a widely used therapy for improving SF viscoelasticity and lubrication, but it is unclear how the effectiveness of HA viscosupplements varies with arthritic endotype.
32577715	2	24	theme	acid	261:264	arg1	viscosupplementation					271:290	Hyaluronic acid (HA) viscosupplementation	250:290	Hyaluronic acid (HA) viscosupplementation	250:290	Hyaluronic acid (HA) viscosupplementation is a widely used therapy for improving SF viscoelasticity and lubrication, but it is unclear how the effectiveness of HA viscosupplements varies with arthritic endotype.
32577715	1	25	theme	synovial	170:177	arg1	fluid					179:183	synovial fluid	170:183	synovial fluid (SF) lubrication	170:200	Inferior synovial lubrication is a hallmark of osteoarthritis (OA), and synovial fluid (SF) lubrication and composition are variable among OA patients.
32577715	1	25	theme	synovial	170:177	arg1	SF					186:187	SF	186:187	SF	186:187	Inferior synovial lubrication is a hallmark of osteoarthritis (OA), and synovial fluid (SF) lubrication and composition are variable among OA patients.
32577715	4	26	dep	rheology	795:802	arg1	tribology					805:813	tribology	805:813	tribology	805:813	The composition (cytokine, HA, and lubricin concentrations) of the SF was measured as well as the mechanical properties (rheology, tribology) of the SF alone and in a 1:1 mixture with the HA viscosupplement.
32577715	8	27	theme	HA	1422:1423	arg1	addition					1410:1417	the addition	1406:1417	the addition of HA	1406:1423	Interestingly, this aberrant behavior was not eliminated by the addition of HA, and it was associated with low concentrations of lubricin.
32577715	9	28	theme	IA	1584:1585	arg1	SF					1587:1588	IA SF	1584:1588	IA SF	1584:1588	Aberrant SF exhibited significantly lower effective viscosities compared to noninflammatory OA and IA SF with typical tribological behavior.
32577715	10	29	theme	arthritis	1690:1698	arg1	endotypes					1677:1685	different endotypes	1667:1685	different endotypes of arthritis	1667:1698	Collectively, these results suggest that different endotypes of arthritis exist with respect to lubrication, which may impact the effectiveness of HA viscosupplements in reducing friction.
32577715	1	30	theme	Inferior	98:105	arg1	lubrication					116:126	Inferior synovial lubrication	98:126	Inferior synovial lubrication	98:126	Inferior synovial lubrication is a hallmark of osteoarthritis (OA), and synovial fluid (SF) lubrication and composition are variable among OA patients.
32577715	1	30	theme	Inferior	98:105	arg1	hallmark					133:140	a hallmark	131:140	a hallmark of osteoarthritis (OA)	131:163	Inferior synovial lubrication is a hallmark of osteoarthritis (OA), and synovial fluid (SF) lubrication and composition are variable among OA patients.
32577715	1	31	theme	fluid	179:183	arg1	lubrication					190:200	synovial fluid (SF) lubrication	170:200	synovial fluid (SF) lubrication	170:200	Inferior synovial lubrication is a hallmark of osteoarthritis (OA), and synovial fluid (SF) lubrication and composition are variable among OA patients.
32577715	2	32	theme	viscosupplements	413:428	arg1	effectiveness					393:405	the effectiveness	389:405	the effectiveness of HA viscosupplements	389:428	Hyaluronic acid (HA) viscosupplementation is a widely used therapy for improving SF viscoelasticity and lubrication, but it is unclear how the effectiveness of HA viscosupplements varies with arthritic endotype.
32577715	1	33	theme	synovial	107:114	arg1	lubrication					116:126	Inferior synovial lubrication	98:126	Inferior synovial lubrication	98:126	Inferior synovial lubrication is a hallmark of osteoarthritis (OA), and synovial fluid (SF) lubrication and composition are variable among OA patients.
32577715	1	33	theme	synovial	107:114	arg1	hallmark					133:140	a hallmark	131:140	a hallmark of osteoarthritis (OA)	131:163	Inferior synovial lubrication is a hallmark of osteoarthritis (OA), and synovial fluid (SF) lubrication and composition are variable among OA patients.
32577715	0	34	theme	Synovial	33:40	arg1	Fluids					42:47	Inflammatory and Noninflammatory Synovial Fluids	0:47	Inflammatory and Noninflammatory Synovial Fluids	0:47	Inflammatory and Noninflammatory Synovial Fluids Exhibit New and Distinct Tribological Endotypes.
32577715	7	35	theme	typical	1226:1232	arg1	behavior					1243:1250	typical Stribeck behavior	1226:1250	typical Stribeck behavior	1226:1250	While some of the IA SF also showed typical Stribeck behavior, a subset showed more erratic behavior with highly variable and larger friction coefficients.
32577715	3	36	theme	HA	528:529	arg1	viscosupplement					531:545	the HA viscosupplement	524:545	the HA viscosupplement	524:545	The objective of this study was to investigate the effects of the HA viscosupplement, Hymovis®, on the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA).
32577715	3	36	theme	HA	528:529	arg1	Hymovis®					548:555	Hymovis®	548:555	Hymovis®	548:555	The objective of this study was to investigate the effects of the HA viscosupplement, Hymovis®, on the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA).
32577715	3	37	with	patients	608:615	arg1	IA					669:670	IA	669:670	IA	669:670	The objective of this study was to investigate the effects of the HA viscosupplement, Hymovis®, on the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA).
32577715	3	37	with	patients	608:615	arg1	OA					638:639	noninflammatory OA	622:639	noninflammatory OA	622:639	The objective of this study was to investigate the effects of the HA viscosupplement, Hymovis®, on the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA).
32577715	3	37	with	patients	608:615	arg1	arthritis					658:666	inflammatory arthritis	645:666	inflammatory arthritis (IA)	645:671	The objective of this study was to investigate the effects of the HA viscosupplement, Hymovis®, on the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA).
32577715	0	38	theme	Noninflammatory	17:31	arg1	Fluids					42:47	Inflammatory and Noninflammatory Synovial Fluids	0:47	Inflammatory and Noninflammatory Synovial Fluids	0:47	Inflammatory and Noninflammatory Synovial Fluids Exhibit New and Distinct Tribological Endotypes.
32577715	6	39	theme	friction	1074:1081	arg1	coefficients					1083:1094	friction coefficients	1074:1094	friction coefficients	1074:1094	In noninflammatory OA SF, friction coefficients followed a typical Stribeck pattern, and their magnitude was decreased by the addition of HA.
32577715	10	40	theme	HA	1773:1774	arg1	viscosupplements					1776:1791	HA viscosupplements	1773:1791	HA viscosupplements	1773:1791	Collectively, these results suggest that different endotypes of arthritis exist with respect to lubrication, which may impact the effectiveness of HA viscosupplements in reducing friction.
32577715	3	41	theme	viscosupplement	531:545	arg1	effects					513:519	the effects	509:519	the effects of the HA viscosupplement, Hymovis®, on the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA)	509:671	The objective of this study was to investigate the effects of the HA viscosupplement, Hymovis®, on the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA).
32577715	6	42	theme	OA	1067:1068	arg1	SF					1070:1071	noninflammatory OA SF	1051:1071	noninflammatory OA SF	1051:1071	In noninflammatory OA SF, friction coefficients followed a typical Stribeck pattern, and their magnitude was decreased by the addition of HA.
32577715	9	43	theme	tribological	1603:1614	arg1	behavior					1616:1623	typical tribological behavior	1595:1623	typical tribological behavior	1595:1623	Aberrant SF exhibited significantly lower effective viscosities compared to noninflammatory OA and IA SF with typical tribological behavior.
32577715	3	44	theme	noninflammatory	622:636	arg1	OA					638:639	noninflammatory OA	622:639	noninflammatory OA	622:639	The objective of this study was to investigate the effects of the HA viscosupplement, Hymovis®, on the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA).
32577715	5	45	theme	SF	927:928	arg1	viscosity					930:938	SF viscosity	927:938	SF viscosity	927:938	Using rotational rheometry, no difference in SF viscosity was detected between disease types, and the addition of HA significantly increased all fluids' viscosities.
32577715	7	46	theme	friction	1323:1330	arg1	coefficients					1332:1343	highly variable and larger friction coefficients	1296:1343	highly variable and larger friction coefficients	1296:1343	While some of the IA SF also showed typical Stribeck behavior, a subset showed more erratic behavior with highly variable and larger friction coefficients.
32577715	9	47	theme	lower	1521:1525	arg1	viscosities					1537:1547	significantly lower effective viscosities	1507:1547	significantly lower effective viscosities	1507:1547	Aberrant SF exhibited significantly lower effective viscosities compared to noninflammatory OA and IA SF with typical tribological behavior.
32577715	8	48	theme	low	1453:1455	arg1	concentrations					1457:1470	low concentrations	1453:1470	low concentrations of lubricin	1453:1482	Interestingly, this aberrant behavior was not eliminated by the addition of HA, and it was associated with low concentrations of lubricin.
32577715	4	49	theme	1:1	841:843	arg1	mixture					845:851	a 1:1 mixture	839:851	a 1:1 mixture with the HA viscosupplement	839:879	The composition (cytokine, HA, and lubricin concentrations) of the SF was measured as well as the mechanical properties (rheology, tribology) of the SF alone and in a 1:1 mixture with the HA viscosupplement.
32577715	2	50	theme	HA	410:411	arg1	viscosupplements					413:428	HA viscosupplements	410:428	HA viscosupplements	410:428	Hyaluronic acid (HA) viscosupplementation is a widely used therapy for improving SF viscoelasticity and lubrication, but it is unclear how the effectiveness of HA viscosupplements varies with arthritic endotype.
32577715	2	51	theme	arthritic	442:450	arg1	endotype					452:459	arthritic endotype	442:459	arthritic endotype	442:459	Hyaluronic acid (HA) viscosupplementation is a widely used therapy for improving SF viscoelasticity and lubrication, but it is unclear how the effectiveness of HA viscosupplements varies with arthritic endotype.
32577715	4	52	theme	SF	823:824	arg1	composition					678:688	The composition	674:688	The composition (cytokine, HA, and lubricin concentrations) of the SF	674:742	The composition (cytokine, HA, and lubricin concentrations) of the SF was measured as well as the mechanical properties (rheology, tribology) of the SF alone and in a 1:1 mixture with the HA viscosupplement.
32577715	4	52	theme	SF	823:824	arg1	rheology					795:802	rheology	795:802	rheology	795:802	The composition (cytokine, HA, and lubricin concentrations) of the SF was measured as well as the mechanical properties (rheology, tribology) of the SF alone and in a 1:1 mixture with the HA viscosupplement.
32577715	4	52	theme	SF	823:824	arg1	properties					783:792	the mechanical properties	768:792	the mechanical properties (rheology, tribology) of the SF alone	768:830	The composition (cytokine, HA, and lubricin concentrations) of the SF was measured as well as the mechanical properties (rheology, tribology) of the SF alone and in a 1:1 mixture with the HA viscosupplement.
32577715	9	53	theme	Aberrant	1485:1492	arg1	SF					1494:1495	Aberrant SF	1485:1495	Aberrant SF	1485:1495	Aberrant SF exhibited significantly lower effective viscosities compared to noninflammatory OA and IA SF with typical tribological behavior.
32577715	0	54	theme	New	57:59	arg1	Endotypes					87:95	New and Distinct Tribological Endotypes	57:95	New and Distinct Tribological Endotypes	57:95	Inflammatory and Noninflammatory Synovial Fluids Exhibit New and Distinct Tribological Endotypes.
32577715	10	55	theme	different	1667:1675	arg1	endotypes					1677:1685	different endotypes	1667:1685	different endotypes of arthritis	1667:1698	Collectively, these results suggest that different endotypes of arthritis exist with respect to lubrication, which may impact the effectiveness of HA viscosupplements in reducing friction.
32577715	3	56	theme	inflammatory	645:656	arg1	IA					669:670	IA	669:670	IA	669:670	The objective of this study was to investigate the effects of the HA viscosupplement, Hymovis®, on the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA).
32577715	3	56	theme	inflammatory	645:656	arg1	arthritis					658:666	inflammatory arthritis	645:666	inflammatory arthritis (IA)	645:671	The objective of this study was to investigate the effects of the HA viscosupplement, Hymovis®, on the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA).
32577715	5	57	theme	disease	961:967	arg1	types					969:973	disease types	961:973	disease types	961:973	Using rotational rheometry, no difference in SF viscosity was detected between disease types, and the addition of HA significantly increased all fluids' viscosities.
32577715	9	58	theme	noninflammatory	1561:1575	arg1	OA					1577:1578	noninflammatory OA	1561:1578	noninflammatory OA	1561:1578	Aberrant SF exhibited significantly lower effective viscosities compared to noninflammatory OA and IA SF with typical tribological behavior.
32577715	9	59	with	SF	1587:1588	arg1	behavior					1616:1623	typical tribological behavior	1595:1623	typical tribological behavior	1595:1623	Aberrant SF exhibited significantly lower effective viscosities compared to noninflammatory OA and IA SF with typical tribological behavior.
32577715	5	60	from	difference	913:922	arg1	viscosity					930:938	SF viscosity	927:938	SF viscosity	927:938	Using rotational rheometry, no difference in SF viscosity was detected between disease types, and the addition of HA significantly increased all fluids' viscosities.
32577715	9	61	theme	effective	1527:1535	arg1	viscosities					1537:1547	significantly lower effective viscosities	1507:1547	significantly lower effective viscosities	1507:1547	Aberrant SF exhibited significantly lower effective viscosities compared to noninflammatory OA and IA SF with typical tribological behavior.
32577715	3	62	theme	diseased	591:598	arg1	SF					600:601	diseased SF	591:601	diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA)	591:671	The objective of this study was to investigate the effects of the HA viscosupplement, Hymovis®, on the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA).
32577715	2	63	theme	Hyaluronic	250:259	arg1	HA					267:268	HA	267:268	HA	267:268	Hyaluronic acid (HA) viscosupplementation is a widely used therapy for improving SF viscoelasticity and lubrication, but it is unclear how the effectiveness of HA viscosupplements varies with arthritic endotype.
32577715	2	63	theme	Hyaluronic	250:259	arg1	acid					261:264	Hyaluronic acid	250:264	Hyaluronic acid (HA) viscosupplementation	250:290	Hyaluronic acid (HA) viscosupplementation is a widely used therapy for improving SF viscoelasticity and lubrication, but it is unclear how the effectiveness of HA viscosupplements varies with arthritic endotype.
32577715	3	64	theme	lubricating	565:575	arg1	properties					577:586	the lubricating properties	561:586	the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA)	561:671	The objective of this study was to investigate the effects of the HA viscosupplement, Hymovis®, on the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA).
32577715	9	65	with	OA	1577:1578	arg1	behavior					1616:1623	typical tribological behavior	1595:1623	typical tribological behavior	1595:1623	Aberrant SF exhibited significantly lower effective viscosities compared to noninflammatory OA and IA SF with typical tribological behavior.
32577715	4	66	dep	composition	678:688	arg1	concentrations					718:731	lubricin concentrations	709:731	lubricin concentrations	709:731	The composition (cytokine, HA, and lubricin concentrations) of the SF was measured as well as the mechanical properties (rheology, tribology) of the SF alone and in a 1:1 mixture with the HA viscosupplement.
32577715	4	66	dep	composition	678:688	arg1	HA					701:702	HA	701:702	HA	701:702	The composition (cytokine, HA, and lubricin concentrations) of the SF was measured as well as the mechanical properties (rheology, tribology) of the SF alone and in a 1:1 mixture with the HA viscosupplement.
32577715	4	66	dep	composition	678:688	arg1	cytokine					691:698	cytokine	691:698	cytokine	691:698	The composition (cytokine, HA, and lubricin concentrations) of the SF was measured as well as the mechanical properties (rheology, tribology) of the SF alone and in a 1:1 mixture with the HA viscosupplement.
32577715	3	67	theme	SF	600:601	arg1	properties					577:586	the lubricating properties	561:586	the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA)	561:671	The objective of this study was to investigate the effects of the HA viscosupplement, Hymovis®, on the lubricating properties of diseased SF from patients with noninflammatory OA and inflammatory arthritis (IA).
32577715	2	68	theme	used	304:307	arg1	therapy					309:315	a widely used therapy	295:315	a widely used therapy for improving SF viscoelasticity and lubrication	295:364	Hyaluronic acid (HA) viscosupplementation is a widely used therapy for improving SF viscoelasticity and lubrication, but it is unclear how the effectiveness of HA viscosupplements varies with arthritic endotype.
32577715	2	68	theme	used	304:307	arg1	viscosupplementation					271:290	Hyaluronic acid (HA) viscosupplementation	250:290	Hyaluronic acid (HA) viscosupplementation	250:290	Hyaluronic acid (HA) viscosupplementation is a widely used therapy for improving SF viscoelasticity and lubrication, but it is unclear how the effectiveness of HA viscosupplements varies with arthritic endotype.
32577715	4	69	theme	SF	741:742	arg1	composition					678:688	The composition	674:688	The composition (cytokine, HA, and lubricin concentrations) of the SF	674:742	The composition (cytokine, HA, and lubricin concentrations) of the SF was measured as well as the mechanical properties (rheology, tribology) of the SF alone and in a 1:1 mixture with the HA viscosupplement.
32577715	4	69	theme	SF	741:742	arg1	properties					783:792	the mechanical properties	768:792	the mechanical properties (rheology, tribology) of the SF alone	768:830	The composition (cytokine, HA, and lubricin concentrations) of the SF was measured as well as the mechanical properties (rheology, tribology) of the SF alone and in a 1:1 mixture with the HA viscosupplement.
32577715	0	70	theme	Tribological	74:85	arg1	Endotypes					87:95	New and Distinct Tribological Endotypes	57:95	New and Distinct Tribological Endotypes	57:95	Inflammatory and Noninflammatory Synovial Fluids Exhibit New and Distinct Tribological Endotypes.
32577715	10	71	theme	viscosupplements	1776:1791	arg1	effectiveness					1756:1768	the effectiveness	1752:1768	the effectiveness of HA viscosupplements in reducing friction	1752:1812	Collectively, these results suggest that different endotypes of arthritis exist with respect to lubrication, which may impact the effectiveness of HA viscosupplements in reducing friction.
32577715	6	72	theme	typical	1107:1113	arg1	pattern					1124:1130	a typical Stribeck pattern	1105:1130	a typical Stribeck pattern	1105:1130	In noninflammatory OA SF, friction coefficients followed a typical Stribeck pattern, and their magnitude was decreased by the addition of HA.
32577715	0	73	theme	Distinct	65:72	arg1	Endotypes					87:95	New and Distinct Tribological Endotypes	57:95	New and Distinct Tribological Endotypes	57:95	Inflammatory and Noninflammatory Synovial Fluids Exhibit New and Distinct Tribological Endotypes.
32577715	7	74	theme	Stribeck	1234:1241	arg1	behavior					1243:1250	typical Stribeck behavior	1226:1250	typical Stribeck behavior	1226:1250	While some of the IA SF also showed typical Stribeck behavior, a subset showed more erratic behavior with highly variable and larger friction coefficients.
34128646	0	0	theme	Peptides	100:107	arg1	Release					75:81	On-Demand Release	65:81	On-Demand Release of Antimicrobial Peptides	65:107	Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels with On-Demand Release of Antimicrobial Peptides for Infected Wound Healing.
34128646	3	1	theme	AMP	708:710	arg1	release					697:703	an acidity-triggered release	676:703	an acidity-triggered release of AMP	676:710	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	8	2	theme	wounds	1593:1598	arg1	management					1553:1562	the management	1549:1562	the management of chronic bacteria-infected wounds	1549:1598	Therefore, this work provides an efficient strategy to fabricate antibiotic-free hydrogel-based biomaterials for the management of chronic bacteria-infected wounds.
34128646	3	3	theme	composite	533:541	arg1	hydrogel					543:550	a hyaluronic acid (HA)-based composite hydrogel	504:550	a hyaluronic acid (HA)-based composite hydrogel	504:550	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	3	3	theme	composite	533:541	arg1	agent					621:625	a cross-linking agent	605:625	a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6)	605:779	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	3	3	theme	composite	533:541	arg1	3KK					597:599	KK(SLKL)3KK]	589:600	KK(SLKL)3KK]	589:600	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	8	4	theme	bacteria-infected	1575:1591	arg1	wounds					1593:1598	chronic bacteria-infected wounds	1567:1598	chronic bacteria-infected wounds	1567:1598	Therefore, this work provides an efficient strategy to fabricate antibiotic-free hydrogel-based biomaterials for the management of chronic bacteria-infected wounds.
34128646	0	5	theme	Antimicrobial	86:98	arg1	Peptides					100:107	Antimicrobial Peptides	86:107	Antimicrobial Peptides	86:107	Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels with On-Demand Release of Antimicrobial Peptides for Infected Wound Healing.
34128646	3	6	theme	acidity-triggered	679:695	arg1	release					697:703	an acidity-triggered release	676:703	an acidity-triggered release of AMP	676:710	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	4	7	theme	due	872:874	arg1	structure					862:870	an antiparallel β-sheet secondary structure	828:870	an antiparallel β-sheet secondary structure due to the alternate arrangement of hydrophobic and hydrophilic residues of amino acids	828:958	During the self-assembly process, AMP adopted an antiparallel β-sheet secondary structure due to the alternate arrangement of hydrophobic and hydrophilic residues of amino acids.
34128646	5	8	theme	oxidized	1071:1078	arg1	HA					1080:1081	oxidized HA	1071:1081	oxidized HA	1071:1081	Owing to Schiff's base formation between the primary amines derived from lysine residues and the aldehydes in oxidized HA, the AMP-HA composite hydrogel exhibited injectability, high biostability, and enhanced mechanical strength.
34128646	0	9	theme	Infected	113:120	arg1	Healing					128:134	Infected Wound Healing	113:134	Infected Wound Healing	113:134	Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels with On-Demand Release of Antimicrobial Peptides for Infected Wound Healing.
34128646	4	10	theme	self-assembly	793:805	arg1	process					807:813	the self-assembly process	789:813	the self-assembly process	789:813	During the self-assembly process, AMP adopted an antiparallel β-sheet secondary structure due to the alternate arrangement of hydrophobic and hydrophilic residues of amino acids.
34128646	4	11	theme	alternate	883:891	arg1	arrangement					893:903	the alternate arrangement	879:903	the alternate arrangement of hydrophobic and hydrophilic residues of amino acids	879:958	During the self-assembly process, AMP adopted an antiparallel β-sheet secondary structure due to the alternate arrangement of hydrophobic and hydrophilic residues of amino acids.
34128646	2	12	theme	on-demand	325:333	arg1	release					335:341	on-demand release	325:341	on-demand release of antibacterial agents	325:365	Hence, antibiotic-free hydrogel-based biomaterials, which exhibit biostability, on-demand release of antibacterial agents, and long-lasting antimicrobial activity, are highly desired for the treatment of chronic bacteria-infected wounds.
34128646	3	13	dep	exhibited	666:674	arg1	environment					726:736	pathological environment	713:736	pathological environment in bacteria-infected wounds	713:764	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	3	13	dep	exhibited	666:674	arg1	∼					770:770	pH ∼ 5.5-5.6	767:778	pH ∼ 5.5-5.6	767:778	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	4	14	theme	acids	954:958	arg1	residues					936:943	hydrophobic and hydrophilic residues	908:943	hydrophobic and hydrophilic residues of amino acids	908:958	During the self-assembly process, AMP adopted an antiparallel β-sheet secondary structure due to the alternate arrangement of hydrophobic and hydrophilic residues of amino acids.
34128646	4	14	theme	acids	954:958	arg1	acids					954:958	amino acids	948:958	amino acids	948:958	During the self-assembly process, AMP adopted an antiparallel β-sheet secondary structure due to the alternate arrangement of hydrophobic and hydrophilic residues of amino acids.
34128646	5	15	theme	AMP-HA	1088:1093	arg1	hydrogel					1105:1112	the AMP-HA composite hydrogel	1084:1112	the AMP-HA composite hydrogel	1084:1112	Owing to Schiff's base formation between the primary amines derived from lysine residues and the aldehydes in oxidized HA, the AMP-HA composite hydrogel exhibited injectability, high biostability, and enhanced mechanical strength.
34128646	3	16	theme	SLKL	592:595	arg1	hydrogel					543:550	a hyaluronic acid (HA)-based composite hydrogel	504:550	a hyaluronic acid (HA)-based composite hydrogel	504:550	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	3	16	theme	SLKL	592:595	arg1	agent					621:625	a cross-linking agent	605:625	a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6)	605:779	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	3	16	theme	SLKL	592:595	arg1	3KK					597:599	KK(SLKL)3KK]	589:600	KK(SLKL)3KK]	589:600	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	5	17	theme	composite	1095:1103	arg1	hydrogel					1105:1112	the AMP-HA composite hydrogel	1084:1112	the AMP-HA composite hydrogel	1084:1112	Owing to Schiff's base formation between the primary amines derived from lysine residues and the aldehydes in oxidized HA, the AMP-HA composite hydrogel exhibited injectability, high biostability, and enhanced mechanical strength.
34128646	4	18	theme	β-sheet	844:850	arg1	structure					862:870	an antiparallel β-sheet secondary structure	828:870	an antiparallel β-sheet secondary structure due to the alternate arrangement of hydrophobic and hydrophilic residues of amino acids	828:958	During the self-assembly process, AMP adopted an antiparallel β-sheet secondary structure due to the alternate arrangement of hydrophobic and hydrophilic residues of amino acids.
34128646	2	19	theme	bacteria-infected	457:473	arg1	wounds					475:480	chronic bacteria-infected wounds	449:480	chronic bacteria-infected wounds	449:480	Hence, antibiotic-free hydrogel-based biomaterials, which exhibit biostability, on-demand release of antibacterial agents, and long-lasting antimicrobial activity, are highly desired for the treatment of chronic bacteria-infected wounds.
34128646	5	20	theme	base	979:982	arg1	formation					984:992	Schiff's base formation	970:992	Schiff's base formation between the primary amines derived from lysine residues and the aldehydes in oxidized HA	970:1081	Owing to Schiff's base formation between the primary amines derived from lysine residues and the aldehydes in oxidized HA, the AMP-HA composite hydrogel exhibited injectability, high biostability, and enhanced mechanical strength.
34128646	5	21	from	amines	1014:1019	arg1	HA					1080:1081	oxidized HA	1071:1081	oxidized HA	1071:1081	Owing to Schiff's base formation between the primary amines derived from lysine residues and the aldehydes in oxidized HA, the AMP-HA composite hydrogel exhibited injectability, high biostability, and enhanced mechanical strength.
34128646	6	22	theme	broad-spectrum	1256:1269	arg1	activity					1285:1292	excellent broad-spectrum antibacterial activity	1246:1292	excellent broad-spectrum antibacterial activity	1246:1292	Importantly, both AMP and the AMP-HA composite showed excellent broad-spectrum antibacterial activity in vitro and in vivo.
34128646	0	23	theme	Wound	122:126	arg1	Healing					128:134	Infected Wound Healing	113:134	Infected Wound Healing	113:134	Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels with On-Demand Release of Antimicrobial Peptides for Infected Wound Healing.
34128646	7	24	dep	on-demand	1370:1378	arg1	thickness					1385:1393	full thickness	1380:1393	full thickness	1380:1393	Specifically, the AMP-HA composite hydrogel exhibited on-demand full thickness wound healing in an infected mice model.
34128646	4	25	theme	antiparallel	831:842	arg1	structure					862:870	an antiparallel β-sheet secondary structure	828:870	an antiparallel β-sheet secondary structure due to the alternate arrangement of hydrophobic and hydrophilic residues of amino acids	828:958	During the self-assembly process, AMP adopted an antiparallel β-sheet secondary structure due to the alternate arrangement of hydrophobic and hydrophilic residues of amino acids.
34128646	3	26	theme	antimicrobial	561:573	arg1	[AMP					583:586	an antimicrobial peptide [AMP	558:586	an antimicrobial peptide [AMP	558:586	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	3	27	theme	pathological	713:724	arg1	environment					726:736	pathological environment	713:736	pathological environment in bacteria-infected wounds	713:764	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	3	27	theme	pathological	713:724	arg1	∼					770:770	pH ∼ 5.5-5.6	767:778	pH ∼ 5.5-5.6	767:778	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	6	28	theme	antibacterial	1271:1283	arg1	activity					1285:1292	excellent broad-spectrum antibacterial activity	1246:1292	excellent broad-spectrum antibacterial activity	1246:1292	Importantly, both AMP and the AMP-HA composite showed excellent broad-spectrum antibacterial activity in vitro and in vivo.
34128646	1	29	from	abuse	150:154	arg1	wounds					177:182	bacteria-infected wounds	159:182	bacteria-infected wounds	159:182	Antibiotics' abuse in bacteria-infected wounds has threatened patients' lives and burdened medical systems.
34128646	2	30	theme	agents	360:365	arg1	activity					399:406	long-lasting antimicrobial activity	372:406	long-lasting antimicrobial activity	372:406	Hence, antibiotic-free hydrogel-based biomaterials, which exhibit biostability, on-demand release of antibacterial agents, and long-lasting antimicrobial activity, are highly desired for the treatment of chronic bacteria-infected wounds.
34128646	2	30	theme	agents	360:365	arg1	biostability					311:322	biostability	311:322	biostability	311:322	Hence, antibiotic-free hydrogel-based biomaterials, which exhibit biostability, on-demand release of antibacterial agents, and long-lasting antimicrobial activity, are highly desired for the treatment of chronic bacteria-infected wounds.
34128646	2	30	theme	agents	360:365	arg1	release					335:341	on-demand release	325:341	on-demand release of antibacterial agents	325:365	Hence, antibiotic-free hydrogel-based biomaterials, which exhibit biostability, on-demand release of antibacterial agents, and long-lasting antimicrobial activity, are highly desired for the treatment of chronic bacteria-infected wounds.
34128646	8	31	theme	chronic	1567:1573	arg1	wounds					1593:1598	chronic bacteria-infected wounds	1567:1598	chronic bacteria-infected wounds	1567:1598	Therefore, this work provides an efficient strategy to fabricate antibiotic-free hydrogel-based biomaterials for the management of chronic bacteria-infected wounds.
34128646	7	32	theme	AMP-HA	1334:1339	arg1	hydrogel					1351:1358	the AMP-HA composite hydrogel	1330:1358	the AMP-HA composite hydrogel	1330:1358	Specifically, the AMP-HA composite hydrogel exhibited on-demand full thickness wound healing in an infected mice model.
34128646	0	33	theme	Injectable	0:9	arg1	Hydrogels					50:58	Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels	0:58	Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels with On-Demand Release of Antimicrobial Peptides for Infected Wound Healing.	0:135	Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels with On-Demand Release of Antimicrobial Peptides for Infected Wound Healing.
34128646	4	34	theme	secondary	852:860	arg1	structure					862:870	an antiparallel β-sheet secondary structure	828:870	an antiparallel β-sheet secondary structure due to the alternate arrangement of hydrophobic and hydrophilic residues of amino acids	828:958	During the self-assembly process, AMP adopted an antiparallel β-sheet secondary structure due to the alternate arrangement of hydrophobic and hydrophilic residues of amino acids.
34128646	2	35	theme	antibacterial	346:358	arg1	agents					360:365	antibacterial agents	346:365	antibacterial agents	346:365	Hence, antibiotic-free hydrogel-based biomaterials, which exhibit biostability, on-demand release of antibacterial agents, and long-lasting antimicrobial activity, are highly desired for the treatment of chronic bacteria-infected wounds.
34128646	5	36	theme	high	1139:1142	arg1	biostability					1144:1155	high biostability	1139:1155	high biostability	1139:1155	Owing to Schiff's base formation between the primary amines derived from lysine residues and the aldehydes in oxidized HA, the AMP-HA composite hydrogel exhibited injectability, high biostability, and enhanced mechanical strength.
34128646	7	37	theme	composite	1341:1349	arg1	hydrogel					1351:1358	the AMP-HA composite hydrogel	1330:1358	the AMP-HA composite hydrogel	1330:1358	Specifically, the AMP-HA composite hydrogel exhibited on-demand full thickness wound healing in an infected mice model.
34128646	0	38	theme	Hyaluronic	28:37	arg1	Hydrogels					50:58	Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels	0:58	Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels with On-Demand Release of Antimicrobial Peptides for Infected Wound Healing.	0:135	Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels with On-Demand Release of Antimicrobial Peptides for Infected Wound Healing.
34128646	3	39	theme	pH	767:768	arg1	environment					726:736	pathological environment	713:736	pathological environment in bacteria-infected wounds	713:764	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	3	39	theme	pH	767:768	arg1	∼					770:770	pH ∼ 5.5-5.6	767:778	pH ∼ 5.5-5.6	767:778	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	4	40	theme	residues	936:943	arg1	arrangement					893:903	the alternate arrangement	879:903	the alternate arrangement of hydrophobic and hydrophilic residues of amino acids	879:958	During the self-assembly process, AMP adopted an antiparallel β-sheet secondary structure due to the alternate arrangement of hydrophobic and hydrophilic residues of amino acids.
34128646	6	41	theme	AMP-HA	1222:1227	arg1	composite					1229:1237	the AMP-HA composite	1218:1237	the AMP-HA composite	1218:1237	Importantly, both AMP and the AMP-HA composite showed excellent broad-spectrum antibacterial activity in vitro and in vivo.
34128646	7	42	theme	mice	1424:1427	arg1	model					1429:1433	an infected mice model	1412:1433	an infected mice model	1412:1433	Specifically, the AMP-HA composite hydrogel exhibited on-demand full thickness wound healing in an infected mice model.
34128646	6	43	theme	excellent	1246:1254	arg1	activity					1285:1292	excellent broad-spectrum antibacterial activity	1246:1292	excellent broad-spectrum antibacterial activity	1246:1292	Importantly, both AMP and the AMP-HA composite showed excellent broad-spectrum antibacterial activity in vitro and in vivo.
34128646	2	44	theme	wounds	475:480	arg1	treatment					436:444	the treatment	432:444	the treatment of chronic bacteria-infected wounds	432:480	Hence, antibiotic-free hydrogel-based biomaterials, which exhibit biostability, on-demand release of antibacterial agents, and long-lasting antimicrobial activity, are highly desired for the treatment of chronic bacteria-infected wounds.
34128646	0	45	theme	pH-Sensitive	15:26	arg1	Hydrogels					50:58	Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels	0:58	Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels with On-Demand Release of Antimicrobial Peptides for Infected Wound Healing.	0:135	Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels with On-Demand Release of Antimicrobial Peptides for Infected Wound Healing.
34128646	3	46	theme	bacteria-infected	741:757	arg1	wounds					759:764	bacteria-infected wounds	741:764	bacteria-infected wounds	741:764	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	4	47	theme	hydrophilic	924:934	arg1	residues					936:943	hydrophobic and hydrophilic residues	908:943	hydrophobic and hydrophilic residues of amino acids	908:958	During the self-assembly process, AMP adopted an antiparallel β-sheet secondary structure due to the alternate arrangement of hydrophobic and hydrophilic residues of amino acids.
34128646	4	47	theme	hydrophilic	924:934	arg1	acids					954:958	amino acids	948:958	amino acids	948:958	During the self-assembly process, AMP adopted an antiparallel β-sheet secondary structure due to the alternate arrangement of hydrophobic and hydrophilic residues of amino acids.
34128646	3	48	theme	base	644:647	arg1	formation					649:657	Schiff's base formation	635:657	Schiff's base formation	635:657	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	3	49	theme	KK	589:590	arg1	hydrogel					543:550	a hyaluronic acid (HA)-based composite hydrogel	504:550	a hyaluronic acid (HA)-based composite hydrogel	504:550	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	3	49	theme	KK	589:590	arg1	agent					621:625	a cross-linking agent	605:625	a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6)	605:779	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	3	49	theme	KK	589:590	arg1	3KK					597:599	KK(SLKL)3KK]	589:600	KK(SLKL)3KK]	589:600	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	1	50	theme	burdened	219:226	arg1	systems					236:242	burdened medical systems	219:242	burdened medical systems	219:242	Antibiotics' abuse in bacteria-infected wounds has threatened patients' lives and burdened medical systems.
34128646	3	51	theme	-based	526:531	arg1	hydrogel					543:550	a hyaluronic acid (HA)-based composite hydrogel	504:550	a hyaluronic acid (HA)-based composite hydrogel	504:550	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	3	51	theme	-based	526:531	arg1	agent					621:625	a cross-linking agent	605:625	a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6)	605:779	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	3	51	theme	-based	526:531	arg1	3KK					597:599	KK(SLKL)3KK]	589:600	KK(SLKL)3KK]	589:600	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	4	52	theme	amino	948:952	arg1	acids					954:958	amino acids	948:958	amino acids	948:958	During the self-assembly process, AMP adopted an antiparallel β-sheet secondary structure due to the alternate arrangement of hydrophobic and hydrophilic residues of amino acids.
34128646	2	53	theme	chronic	449:455	arg1	wounds					475:480	chronic bacteria-infected wounds	449:480	chronic bacteria-infected wounds	449:480	Hence, antibiotic-free hydrogel-based biomaterials, which exhibit biostability, on-demand release of antibacterial agents, and long-lasting antimicrobial activity, are highly desired for the treatment of chronic bacteria-infected wounds.
34128646	2	54	theme	antibiotic-free	252:266	arg1	biomaterials					283:294	antibiotic-free hydrogel-based biomaterials	252:294	antibiotic-free hydrogel-based biomaterials	252:294	Hence, antibiotic-free hydrogel-based biomaterials, which exhibit biostability, on-demand release of antibacterial agents, and long-lasting antimicrobial activity, are highly desired for the treatment of chronic bacteria-infected wounds.
34128646	1	55	theme	medical	228:234	arg1	systems					236:242	burdened medical systems	219:242	burdened medical systems	219:242	Antibiotics' abuse in bacteria-infected wounds has threatened patients' lives and burdened medical systems.
34128646	0	56	theme	Acid-Based	39:48	arg1	Hydrogels					50:58	Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels	0:58	Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels with On-Demand Release of Antimicrobial Peptides for Infected Wound Healing.	0:135	Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels with On-Demand Release of Antimicrobial Peptides for Infected Wound Healing.
34128646	3	57	from	environment	726:736	arg1	wounds					759:764	bacteria-infected wounds	741:764	bacteria-infected wounds	741:764	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	5	58	theme	mechanical	1171:1180	arg1	strength					1182:1189	enhanced mechanical strength	1162:1189	enhanced mechanical strength	1162:1189	Owing to Schiff's base formation between the primary amines derived from lysine residues and the aldehydes in oxidized HA, the AMP-HA composite hydrogel exhibited injectability, high biostability, and enhanced mechanical strength.
34128646	0	59	theme	On-Demand	65:73	arg1	Release					75:81	On-Demand Release	65:81	On-Demand Release of Antimicrobial Peptides	65:107	Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels with On-Demand Release of Antimicrobial Peptides for Infected Wound Healing.
34128646	7	60	theme	on-demand	1370:1378	arg1	healing					1401:1407	on-demand full thickness wound healing	1370:1407	on-demand full thickness wound healing	1370:1407	Specifically, the AMP-HA composite hydrogel exhibited on-demand full thickness wound healing in an infected mice model.
34128646	5	61	theme	lysine	1034:1039	arg1	residues					1041:1048	lysine residues	1034:1048	lysine residues	1034:1048	Owing to Schiff's base formation between the primary amines derived from lysine residues and the aldehydes in oxidized HA, the AMP-HA composite hydrogel exhibited injectability, high biostability, and enhanced mechanical strength.
34128646	2	62	theme	antimicrobial	385:397	arg1	activity					399:406	long-lasting antimicrobial activity	372:406	long-lasting antimicrobial activity	372:406	Hence, antibiotic-free hydrogel-based biomaterials, which exhibit biostability, on-demand release of antibacterial agents, and long-lasting antimicrobial activity, are highly desired for the treatment of chronic bacteria-infected wounds.
34128646	5	63	theme	enhanced	1162:1169	arg1	strength					1182:1189	enhanced mechanical strength	1162:1189	enhanced mechanical strength	1162:1189	Owing to Schiff's base formation between the primary amines derived from lysine residues and the aldehydes in oxidized HA, the AMP-HA composite hydrogel exhibited injectability, high biostability, and enhanced mechanical strength.
34128646	8	64	theme	antibiotic-free	1501:1515	arg1	biomaterials					1532:1543	antibiotic-free hydrogel-based biomaterials	1501:1543	antibiotic-free hydrogel-based biomaterials	1501:1543	Therefore, this work provides an efficient strategy to fabricate antibiotic-free hydrogel-based biomaterials for the management of chronic bacteria-infected wounds.
34128646	1	65	dep	patients	199:206	arg1	lives					209:213	lives	209:213	lives	209:213	Antibiotics' abuse in bacteria-infected wounds has threatened patients' lives and burdened medical systems.
34128646	1	65	dep	patients	199:206	arg1	systems					236:242	burdened medical systems	219:242	burdened medical systems	219:242	Antibiotics' abuse in bacteria-infected wounds has threatened patients' lives and burdened medical systems.
34128646	2	66	theme	long-lasting	372:383	arg1	activity					399:406	long-lasting antimicrobial activity	372:406	long-lasting antimicrobial activity	372:406	Hence, antibiotic-free hydrogel-based biomaterials, which exhibit biostability, on-demand release of antibacterial agents, and long-lasting antimicrobial activity, are highly desired for the treatment of chronic bacteria-infected wounds.
34128646	3	67	theme	cross-linking	607:619	arg1	hydrogel					543:550	a hyaluronic acid (HA)-based composite hydrogel	504:550	a hyaluronic acid (HA)-based composite hydrogel	504:550	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	3	67	theme	cross-linking	607:619	arg1	agent					621:625	a cross-linking agent	605:625	a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6)	605:779	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	3	67	theme	cross-linking	607:619	arg1	3KK					597:599	KK(SLKL)3KK]	589:600	KK(SLKL)3KK]	589:600	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	7	68	theme	full	1380:1383	arg1	thickness					1385:1393	full thickness	1380:1393	full thickness	1380:1393	Specifically, the AMP-HA composite hydrogel exhibited on-demand full thickness wound healing in an infected mice model.
34128646	7	69	theme	infected	1415:1422	arg1	model					1429:1433	an infected mice model	1412:1433	an infected mice model	1412:1433	Specifically, the AMP-HA composite hydrogel exhibited on-demand full thickness wound healing in an infected mice model.
34128646	4	70	theme	hydrophobic	908:918	arg1	residues					936:943	hydrophobic and hydrophilic residues	908:943	hydrophobic and hydrophilic residues of amino acids	908:958	During the self-assembly process, AMP adopted an antiparallel β-sheet secondary structure due to the alternate arrangement of hydrophobic and hydrophilic residues of amino acids.
34128646	4	70	theme	hydrophobic	908:918	arg1	acids					954:958	amino acids	948:958	amino acids	948:958	During the self-assembly process, AMP adopted an antiparallel β-sheet secondary structure due to the alternate arrangement of hydrophobic and hydrophilic residues of amino acids.
34128646	8	71	theme	efficient	1469:1477	arg1	strategy					1479:1486	an efficient strategy	1466:1486	an efficient strategy to fabricate antibiotic-free hydrogel-based biomaterials for the management of chronic bacteria-infected wounds	1466:1598	Therefore, this work provides an efficient strategy to fabricate antibiotic-free hydrogel-based biomaterials for the management of chronic bacteria-infected wounds.
34128646	3	72	theme	peptide	575:581	arg1	[AMP					583:586	an antimicrobial peptide [AMP	558:586	an antimicrobial peptide [AMP	558:586	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	3	73	theme	acid	517:520	arg1	hydrogel					543:550	a hyaluronic acid (HA)-based composite hydrogel	504:550	a hyaluronic acid (HA)-based composite hydrogel	504:550	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	3	73	theme	acid	517:520	arg1	agent					621:625	a cross-linking agent	605:625	a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6)	605:779	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	3	73	theme	acid	517:520	arg1	3KK					597:599	KK(SLKL)3KK]	589:600	KK(SLKL)3KK]	589:600	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	5	74	attach	derived	1021:1027	arg2	amines					1014:1019	the primary amines	1002:1019	the primary amines derived from lysine residues	1002:1048	Owing to Schiff's base formation between the primary amines derived from lysine residues and the aldehydes in oxidized HA, the AMP-HA composite hydrogel exhibited injectability, high biostability, and enhanced mechanical strength.
34128646	5	74	attach	derived	1021:1027	arg1	residues					1041:1048	lysine residues	1034:1048	lysine residues	1034:1048	Owing to Schiff's base formation between the primary amines derived from lysine residues and the aldehydes in oxidized HA, the AMP-HA composite hydrogel exhibited injectability, high biostability, and enhanced mechanical strength.
34128646	5	75	from	aldehydes	1058:1066	arg1	HA					1080:1081	oxidized HA	1071:1081	oxidized HA	1071:1081	Owing to Schiff's base formation between the primary amines derived from lysine residues and the aldehydes in oxidized HA, the AMP-HA composite hydrogel exhibited injectability, high biostability, and enhanced mechanical strength.
34128646	8	76	theme	hydrogel-based	1517:1530	arg1	biomaterials					1532:1543	antibiotic-free hydrogel-based biomaterials	1501:1543	antibiotic-free hydrogel-based biomaterials	1501:1543	Therefore, this work provides an efficient strategy to fabricate antibiotic-free hydrogel-based biomaterials for the management of chronic bacteria-infected wounds.
34128646	5	77	theme	primary	1006:1012	arg1	amines					1014:1019	the primary amines	1002:1019	the primary amines derived from lysine residues	1002:1048	Owing to Schiff's base formation between the primary amines derived from lysine residues and the aldehydes in oxidized HA, the AMP-HA composite hydrogel exhibited injectability, high biostability, and enhanced mechanical strength.
34128646	3	78	theme	hyaluronic	506:515	arg1	HA					523:524	HA	523:524	HA	523:524	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	3	78	theme	hyaluronic	506:515	arg1	acid					517:520	a hyaluronic acid	504:520	a hyaluronic acid (HA)-based composite hydrogel	504:550	Herein, we developed a hyaluronic acid (HA)-based composite hydrogel, with an antimicrobial peptide [AMP, KK(SLKL)3KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of AMP (pathological environment in bacteria-infected wounds, pH ∼ 5.5-5.6).
34128646	7	79	theme	wound	1395:1399	arg1	healing					1401:1407	on-demand full thickness wound healing	1370:1407	on-demand full thickness wound healing	1370:1407	Specifically, the AMP-HA composite hydrogel exhibited on-demand full thickness wound healing in an infected mice model.
34128646	2	80	theme	hydrogel-based	268:281	arg1	biomaterials					283:294	antibiotic-free hydrogel-based biomaterials	252:294	antibiotic-free hydrogel-based biomaterials	252:294	Hence, antibiotic-free hydrogel-based biomaterials, which exhibit biostability, on-demand release of antibacterial agents, and long-lasting antimicrobial activity, are highly desired for the treatment of chronic bacteria-infected wounds.
34128646	0	81	with	Hydrogels	50:58	arg1	Release					75:81	On-Demand Release	65:81	On-Demand Release of Antimicrobial Peptides	65:107	Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels with On-Demand Release of Antimicrobial Peptides for Infected Wound Healing.
34128646	1	82	theme	bacteria-infected	159:175	arg1	wounds					177:182	bacteria-infected wounds	159:182	bacteria-infected wounds	159:182	Antibiotics' abuse in bacteria-infected wounds has threatened patients' lives and burdened medical systems.
34696590	4	0	from	pollutants	781:790	arg1	systems					801:807	water systems	795:807	water systems	795:807	Herein, we describe a novel approach using fungal melanin for developing melanized-cationic cellulose nanofiber (melanized-C-CNF) foams that can successfully remove pollutants in water systems.
34696590	2	1	theme	global	353:358	arg1	importance					360:369	global importance	353:369	global importance	353:369	Finding solutions to tackle water pollution is urgently required and is of global importance.
34696590	1	2	theme	developing	256:265	arg1	countries					267:275	some developing countries	251:275	some developing countries	251:275	In recent years, water pollution has developed into a severe environmental and public health problem due to rapid urbanization and industrialization, especially in some developing countries.
34696590	2	3	theme	tackle	299:304	arg1	pollution					312:320	tackle water pollution	299:320	tackle water pollution	299:320	Finding solutions to tackle water pollution is urgently required and is of global importance.
34696590	2	4	theme	water	306:310	arg1	pollution					312:320	tackle water pollution	299:320	tackle water pollution	299:320	Finding solutions to tackle water pollution is urgently required and is of global importance.
34696590	3	5	theme	pollutants	546:555	arg1	removal					535:541	the removal	531:541	the removal of pollutants down to ppm/ppb concentrations	531:586	Currently, a range of water treatment methods are available; however, a water remediation process that is simple, inexpensive, eco-friendly, and effective for the removal of pollutants down to ppm/ppb concentrations has long been sought after.
34696590	6	6	theme	melanin	1132:1138	arg1	properties					1111:1120	the unique properties	1100:1120	the unique properties of fungal melanin	1100:1138	This work highlights the opportunity to combine both the advanced features of sustainable polymers such as cellulose and the unique properties of fungal melanin to manufacture biohybrid composites for water purification.
34696590	6	6	theme	melanin	1132:1138	arg1	features					1045:1052	the advanced features	1032:1052	the advanced features of sustainable polymers such as cellulose	1032:1094	This work highlights the opportunity to combine both the advanced features of sustainable polymers such as cellulose and the unique properties of fungal melanin to manufacture biohybrid composites for water purification.
34696590	6	7	theme	biohybrid	1155:1163	arg1	composites					1165:1174	biohybrid composites	1155:1174	biohybrid composites for water purification	1155:1197	This work highlights the opportunity to combine both the advanced features of sustainable polymers such as cellulose and the unique properties of fungal melanin to manufacture biohybrid composites for water purification.
34696590	6	8	theme	polymers	1069:1076	arg1	properties					1111:1120	the unique properties	1100:1120	the unique properties of fungal melanin	1100:1138	This work highlights the opportunity to combine both the advanced features of sustainable polymers such as cellulose and the unique properties of fungal melanin to manufacture biohybrid composites for water purification.
34696590	6	8	theme	polymers	1069:1076	arg1	features					1045:1052	the advanced features	1032:1052	the advanced features of sustainable polymers such as cellulose	1032:1094	This work highlights the opportunity to combine both the advanced features of sustainable polymers such as cellulose and the unique properties of fungal melanin to manufacture biohybrid composites for water purification.
34696590	1	9	theme	public	166:171	arg1	problem					180:186	a severe environmental and public health problem	139:186	problem	180:186	In recent years, water pollution has developed into a severe environmental and public health problem due to rapid urbanization and industrialization, especially in some developing countries.
34696590	5	10	theme	high	912:915	arg1	practicability					917:930	high practicability	912:930	high practicability for adsorbing the cationic dye crystal violet	912:976	The foam can be recycled several times while retaining its adsorption/desorption property, indicating high practicability for adsorbing the cationic dye crystal violet.
34696590	0	11	theme	Bioinspired	49:59	arg1	Removal					61:67	Bioinspired Removal	49:67	Bioinspired Removal of Cationic Dyes	49:84	Melanized-Cationic Cellulose Nanofiber Foams for Bioinspired Removal of Cationic Dyes.
34696590	2	12	theme	Finding	278:284	arg1	solutions					286:294	Finding solutions	278:294	Finding solutions to tackle water pollution	278:320	Finding solutions to tackle water pollution is urgently required and is of global importance.
34696590	6	13	theme	unique	1104:1109	arg1	properties					1111:1120	the unique properties	1100:1120	the unique properties of fungal melanin	1100:1138	This work highlights the opportunity to combine both the advanced features of sustainable polymers such as cellulose and the unique properties of fungal melanin to manufacture biohybrid composites for water purification.
34696590	1	14	theme	health	173:178	arg1	problem					180:186	a severe environmental and public health problem	139:186	problem	180:186	In recent years, water pollution has developed into a severe environmental and public health problem due to rapid urbanization and industrialization, especially in some developing countries.
34696590	1	15	theme	recent	90:95	arg1	years					97:101	recent years	90:101	recent years	90:101	In recent years, water pollution has developed into a severe environmental and public health problem due to rapid urbanization and industrialization, especially in some developing countries.
34696590	0	16	theme	Melanized-Cationic	0:17	arg1	Cellulose					19:27	Melanized-Cationic Cellulose	0:27	Melanized-Cationic Cellulose	0:27	Melanized-Cationic Cellulose Nanofiber Foams for Bioinspired Removal of Cationic Dyes.
34696590	3	17	theme	down	557:560	arg1	pollutants					546:555	pollutants	546:555	pollutants down to ppm/ppb concentrations	546:586	Currently, a range of water treatment methods are available; however, a water remediation process that is simple, inexpensive, eco-friendly, and effective for the removal of pollutants down to ppm/ppb concentrations has long been sought after.
34696590	4	18	theme	novel	638:642	arg1	approach					644:651	a novel approach	636:651	a novel approach using fungal melanin for developing melanized-cationic cellulose nanofiber (melanized-C-CNF) foams that can successfully remove pollutants in water systems	636:807	Herein, we describe a novel approach using fungal melanin for developing melanized-cationic cellulose nanofiber (melanized-C-CNF) foams that can successfully remove pollutants in water systems.
34696590	5	19	theme	crystal	963:969	arg1	violet					971:976	crystal violet	963:976	the cationic dye crystal violet	946:976	The foam can be recycled several times while retaining its adsorption/desorption property, indicating high practicability for adsorbing the cationic dye crystal violet.
34696590	3	20	theme	remediation	450:460	arg1	simple					478:483	simple	478:483	simple	478:483	Currently, a range of water treatment methods are available; however, a water remediation process that is simple, inexpensive, eco-friendly, and effective for the removal of pollutants down to ppm/ppb concentrations has long been sought after.
34696590	3	20	theme	remediation	450:460	arg1	process					462:468	a water remediation process	442:468	a water remediation process that is simple, inexpensive, eco-friendly, and effective for the removal of pollutants down to ppm/ppb concentrations	442:586	Currently, a range of water treatment methods are available; however, a water remediation process that is simple, inexpensive, eco-friendly, and effective for the removal of pollutants down to ppm/ppb concentrations has long been sought after.
34696590	6	21	theme	water	1180:1184	arg1	purification					1186:1197	water purification	1180:1197	water purification	1180:1197	This work highlights the opportunity to combine both the advanced features of sustainable polymers such as cellulose and the unique properties of fungal melanin to manufacture biohybrid composites for water purification.
34696590	1	22	theme	water	104:108	arg1	pollution					110:118	water pollution	104:118	water pollution	104:118	In recent years, water pollution has developed into a severe environmental and public health problem due to rapid urbanization and industrialization, especially in some developing countries.
34696590	1	23	theme	rapid	195:199	arg1	urbanization					201:212	rapid urbanization	195:212	rapid urbanization	195:212	In recent years, water pollution has developed into a severe environmental and public health problem due to rapid urbanization and industrialization, especially in some developing countries.
34696590	3	24	theme	methods	410:416	arg1	range					385:389	a range	383:389	a range of water treatment methods	383:416	Currently, a range of water treatment methods are available; however, a water remediation process that is simple, inexpensive, eco-friendly, and effective for the removal of pollutants down to ppm/ppb concentrations has long been sought after.
34696590	3	24	theme	methods	410:416	arg1	available					422:430	available	422:430	available	422:430	Currently, a range of water treatment methods are available; however, a water remediation process that is simple, inexpensive, eco-friendly, and effective for the removal of pollutants down to ppm/ppb concentrations has long been sought after.
34696590	6	25	theme	sustainable	1057:1067	arg1	polymers					1069:1076	sustainable polymers	1057:1076	sustainable polymers such as cellulose	1057:1094	This work highlights the opportunity to combine both the advanced features of sustainable polymers such as cellulose and the unique properties of fungal melanin to manufacture biohybrid composites for water purification.
34696590	6	25	theme	sustainable	1057:1067	arg1	cellulose					1086:1094	cellulose	1086:1094	cellulose	1086:1094	This work highlights the opportunity to combine both the advanced features of sustainable polymers such as cellulose and the unique properties of fungal melanin to manufacture biohybrid composites for water purification.
34696590	5	26	theme	adsorption/desorption	869:889	arg1	property					891:898	its adsorption/desorption property	865:898	its adsorption/desorption property	865:898	The foam can be recycled several times while retaining its adsorption/desorption property, indicating high practicability for adsorbing the cationic dye crystal violet.
34696590	5	27	theme	several	835:841	arg1	times					843:847	several times	835:847	several times	835:847	The foam can be recycled several times while retaining its adsorption/desorption property, indicating high practicability for adsorbing the cationic dye crystal violet.
34696590	5	28	theme	cationic	950:957	arg1	dye					959:961	the cationic dye	946:961	the cationic dye crystal violet	946:976	The foam can be recycled several times while retaining its adsorption/desorption property, indicating high practicability for adsorbing the cationic dye crystal violet.
34696590	1	29	from	urbanization	201:212	arg1	countries					267:275	some developing countries	251:275	some developing countries	251:275	In recent years, water pollution has developed into a severe environmental and public health problem due to rapid urbanization and industrialization, especially in some developing countries.
34696590	3	30	theme	water	444:448	arg1	simple					478:483	simple	478:483	simple	478:483	Currently, a range of water treatment methods are available; however, a water remediation process that is simple, inexpensive, eco-friendly, and effective for the removal of pollutants down to ppm/ppb concentrations has long been sought after.
34696590	3	30	theme	water	444:448	arg1	process					462:468	a water remediation process	442:468	a water remediation process that is simple, inexpensive, eco-friendly, and effective for the removal of pollutants down to ppm/ppb concentrations	442:586	Currently, a range of water treatment methods are available; however, a water remediation process that is simple, inexpensive, eco-friendly, and effective for the removal of pollutants down to ppm/ppb concentrations has long been sought after.
34696590	3	31	theme	water	394:398	arg1	methods					410:416	water treatment methods	394:416	water treatment methods	394:416	Currently, a range of water treatment methods are available; however, a water remediation process that is simple, inexpensive, eco-friendly, and effective for the removal of pollutants down to ppm/ppb concentrations has long been sought after.
34696590	6	32	theme	fungal	1125:1130	arg1	melanin					1132:1138	fungal melanin	1125:1138	fungal melanin	1125:1138	This work highlights the opportunity to combine both the advanced features of sustainable polymers such as cellulose and the unique properties of fungal melanin to manufacture biohybrid composites for water purification.
34696590	4	33	theme	melanized-cationic	689:706	arg1	melanized-C-CNF					729:743	melanized-C-CNF	729:743	melanized-C-CNF	729:743	Herein, we describe a novel approach using fungal melanin for developing melanized-cationic cellulose nanofiber (melanized-C-CNF) foams that can successfully remove pollutants in water systems.
34696590	4	33	theme	melanized-cationic	689:706	arg1	nanofiber					718:726	melanized-cationic cellulose nanofiber	689:726	melanized-cationic cellulose nanofiber (melanized-C-CNF) foams that can successfully remove pollutants in water systems	689:807	Herein, we describe a novel approach using fungal melanin for developing melanized-cationic cellulose nanofiber (melanized-C-CNF) foams that can successfully remove pollutants in water systems.
34696590	3	34	theme	treatment	400:408	arg1	methods					410:416	water treatment methods	394:416	water treatment methods	394:416	Currently, a range of water treatment methods are available; however, a water remediation process that is simple, inexpensive, eco-friendly, and effective for the removal of pollutants down to ppm/ppb concentrations has long been sought after.
34696590	5	35	dep	dye	959:961	arg1	violet					971:976	crystal violet	963:976	the cationic dye crystal violet	946:976	The foam can be recycled several times while retaining its adsorption/desorption property, indicating high practicability for adsorbing the cationic dye crystal violet.
34696590	3	36	theme	ppm/ppb	565:571	arg1	concentrations					573:586	ppm/ppb concentrations	565:586	ppm/ppb concentrations	565:586	Currently, a range of water treatment methods are available; however, a water remediation process that is simple, inexpensive, eco-friendly, and effective for the removal of pollutants down to ppm/ppb concentrations has long been sought after.
34696590	1	37	from	industrialization	218:234	arg1	countries					267:275	some developing countries	251:275	some developing countries	251:275	In recent years, water pollution has developed into a severe environmental and public health problem due to rapid urbanization and industrialization, especially in some developing countries.
34696590	4	38	theme	nanofiber	718:726	arg1	foams					746:750	melanized-cationic cellulose nanofiber (melanized-C-CNF) foams	689:750	melanized-cationic cellulose nanofiber (melanized-C-CNF) foams that can successfully remove pollutants in water systems	689:807	Herein, we describe a novel approach using fungal melanin for developing melanized-cationic cellulose nanofiber (melanized-C-CNF) foams that can successfully remove pollutants in water systems.
34696590	4	39	theme	cellulose	708:716	arg1	melanized-C-CNF					729:743	melanized-C-CNF	729:743	melanized-C-CNF	729:743	Herein, we describe a novel approach using fungal melanin for developing melanized-cationic cellulose nanofiber (melanized-C-CNF) foams that can successfully remove pollutants in water systems.
34696590	4	39	theme	cellulose	708:716	arg1	nanofiber					718:726	melanized-cationic cellulose nanofiber	689:726	melanized-cationic cellulose nanofiber (melanized-C-CNF) foams that can successfully remove pollutants in water systems	689:807	Herein, we describe a novel approach using fungal melanin for developing melanized-cationic cellulose nanofiber (melanized-C-CNF) foams that can successfully remove pollutants in water systems.
34696590	6	40	theme	advanced	1036:1043	arg1	features					1045:1052	the advanced features	1032:1052	the advanced features of sustainable polymers such as cellulose	1032:1094	This work highlights the opportunity to combine both the advanced features of sustainable polymers such as cellulose and the unique properties of fungal melanin to manufacture biohybrid composites for water purification.
34696590	0	41	theme	Dyes	81:84	arg1	Removal					61:67	Bioinspired Removal	49:67	Bioinspired Removal of Cationic Dyes	49:84	Melanized-Cationic Cellulose Nanofiber Foams for Bioinspired Removal of Cationic Dyes.
34696590	4	42	theme	fungal	659:664	arg1	melanin					666:672	fungal melanin	659:672	fungal melanin for developing melanized-cationic cellulose nanofiber (melanized-C-CNF) foams that can successfully remove pollutants in water systems	659:807	Herein, we describe a novel approach using fungal melanin for developing melanized-cationic cellulose nanofiber (melanized-C-CNF) foams that can successfully remove pollutants in water systems.
34696590	0	43	theme	Cationic	72:79	arg1	Dyes					81:84	Cationic Dyes	72:84	Cationic Dyes	72:84	Melanized-Cationic Cellulose Nanofiber Foams for Bioinspired Removal of Cationic Dyes.
34696590	4	44	theme	water	795:799	arg1	systems					801:807	water systems	795:807	water systems	795:807	Herein, we describe a novel approach using fungal melanin for developing melanized-cationic cellulose nanofiber (melanized-C-CNF) foams that can successfully remove pollutants in water systems.
34534796	0	0	from	insights	12:19	arg1	evolution					28:36	the evolution	24:36	the evolution of sonicated cellulose nanocrystal dispersions	24:83	Rheological insights on the evolution of sonicated cellulose nanocrystal dispersions.
34534796	3	1	theme	process	536:542	arg1	influence					508:516	the influence	504:516	the influence of the dispersing process	504:542	While the optimized parameters for sonication are now well defined for small volumes of low concentration CNC suspensions, a deeper understanding of the influence of the dispersing process is needed to work with larger volumes, at higher concentrations.
34534796	5	2	theme	same	921:924	arg1	concentration					926:938	the same concentration	917:938	the same concentration	917:938	After considering the importance of the measurement sampling volume, the behavior of a more concentrated suspension (6.4 wt%) is examined and compared with a never-dried suspension of the same concentration to validate the dispersion state.
34534796	2	3	used	used	259:262	arg2	Ultrasonication					231:245	Ultrasonication	231:245	Ultrasonication	231:245	Ultrasonication is commonly used to disperse CNCs in water, bringing enough energy to the suspension to break agglomerates.
34534796	5	4	theme	suspension	838:847	arg1	behavior					806:813	the behavior	802:813	the behavior of a more concentrated suspension (6.4 wt%)	802:857	After considering the importance of the measurement sampling volume, the behavior of a more concentrated suspension (6.4 wt%) is examined and compared with a never-dried suspension of the same concentration to validate the dispersion state.
34534796	5	5	theme	sampling	785:792	arg1	volume					794:799	the measurement sampling volume	769:799	the measurement sampling volume	769:799	After considering the importance of the measurement sampling volume, the behavior of a more concentrated suspension (6.4 wt%) is examined and compared with a never-dried suspension of the same concentration to validate the dispersion state.
34534796	1	6	theme	Cellulose	86:94	arg1	biomaterials					130:141	promising biomaterials	120:141	promising biomaterials	120:141	Cellulose nanocrystals (CNCs) are promising biomaterials, but their tendency to agglomerate when dried limits their use in several applications.
34534796	1	6	theme	Cellulose	86:94	arg1	CNCs					110:113	CNCs	110:113	CNCs	110:113	Cellulose nanocrystals (CNCs) are promising biomaterials, but their tendency to agglomerate when dried limits their use in several applications.
34534796	1	6	theme	Cellulose	86:94	arg1	nanocrystals					96:107	Cellulose nanocrystals	86:107	Cellulose nanocrystals (CNCs)	86:114	Cellulose nanocrystals (CNCs) are promising biomaterials, but their tendency to agglomerate when dried limits their use in several applications.
34534796	5	7	theme	volume	794:799	arg1	importance					755:764	the importance	751:764	the importance of the measurement sampling volume	751:799	After considering the importance of the measurement sampling volume, the behavior of a more concentrated suspension (6.4 wt%) is examined and compared with a never-dried suspension of the same concentration to validate the dispersion state.
34534796	4	8	theme	CNC	717:719	arg1	suspension					721:730	a 3.2 wt% CNC suspension	707:730	a 3.2 wt% CNC suspension	707:730	Herein, rheology is used to define the distribution and dispersion states upon ultrasonication of a 3.2 wt% CNC suspension.
34534796	0	9	theme	Rheological	0:10	arg1	insights					12:19	Rheological insights	0:19	Rheological insights on the evolution of sonicated cellulose nanocrystal dispersions	0:83	Rheological insights on the evolution of sonicated cellulose nanocrystal dispersions.
34534796	4	10	theme	%	715:715	arg1	suspension					721:730	a 3.2 wt% CNC suspension	707:730	a 3.2 wt% CNC suspension	707:730	Herein, rheology is used to define the distribution and dispersion states upon ultrasonication of a 3.2 wt% CNC suspension.
34534796	3	11	theme	small	426:430	arg1	volumes					432:438	small volumes	426:438	small volumes of low concentration CNC suspensions	426:475	While the optimized parameters for sonication are now well defined for small volumes of low concentration CNC suspensions, a deeper understanding of the influence of the dispersing process is needed to work with larger volumes, at higher concentrations.
34534796	3	12	theme	optimized	365:373	arg1	parameters					375:384	the optimized parameters	361:384	the optimized parameters for sonication	361:399	While the optimized parameters for sonication are now well defined for small volumes of low concentration CNC suspensions, a deeper understanding of the influence of the dispersing process is needed to work with larger volumes, at higher concentrations.
34534796	5	13	theme	measurement	773:783	arg1	volume					794:799	the measurement sampling volume	769:799	the measurement sampling volume	769:799	After considering the importance of the measurement sampling volume, the behavior of a more concentrated suspension (6.4 wt%) is examined and compared with a never-dried suspension of the same concentration to validate the dispersion state.
34534796	3	14	theme	CNC	461:463	arg1	suspensions					465:475	low concentration CNC suspensions	443:475	low concentration CNC suspensions	443:475	While the optimized parameters for sonication are now well defined for small volumes of low concentration CNC suspensions, a deeper understanding of the influence of the dispersing process is needed to work with larger volumes, at higher concentrations.
34534796	4	15	theme	suspension	721:730	arg1	states					676:681	dispersion states	665:681	dispersion states	665:681	Herein, rheology is used to define the distribution and dispersion states upon ultrasonication of a 3.2 wt% CNC suspension.
34534796	4	15	theme	suspension	721:730	arg1	distribution					648:659	distribution	648:659	distribution	648:659	Herein, rheology is used to define the distribution and dispersion states upon ultrasonication of a 3.2 wt% CNC suspension.
34534796	3	16	theme	suspensions	465:475	arg1	volumes					432:438	small volumes	426:438	small volumes of low concentration CNC suspensions	426:475	While the optimized parameters for sonication are now well defined for small volumes of low concentration CNC suspensions, a deeper understanding of the influence of the dispersing process is needed to work with larger volumes, at higher concentrations.
34534796	3	17	theme	low	443:445	arg1	suspensions					465:475	low concentration CNC suspensions	443:475	low concentration CNC suspensions	443:475	While the optimized parameters for sonication are now well defined for small volumes of low concentration CNC suspensions, a deeper understanding of the influence of the dispersing process is needed to work with larger volumes, at higher concentrations.
34534796	4	18	theme	dispersion	665:674	arg1	states					676:681	dispersion states	665:681	dispersion states	665:681	Herein, rheology is used to define the distribution and dispersion states upon ultrasonication of a 3.2 wt% CNC suspension.
34534796	5	19	theme	concentrated	825:836	arg1	suspension					838:847	a more concentrated suspension	818:847	a more concentrated suspension (6.4 wt%)	818:857	After considering the importance of the measurement sampling volume, the behavior of a more concentrated suspension (6.4 wt%) is examined and compared with a never-dried suspension of the same concentration to validate the dispersion state.
34534796	5	19	theme	concentrated	825:836	arg1	%					856:856	6.4 wt%	850:856	6.4 wt%	850:856	After considering the importance of the measurement sampling volume, the behavior of a more concentrated suspension (6.4 wt%) is examined and compared with a never-dried suspension of the same concentration to validate the dispersion state.
34534796	3	20	theme	concentration	447:459	arg1	suspensions					465:475	low concentration CNC suspensions	443:475	low concentration CNC suspensions	443:475	While the optimized parameters for sonication are now well defined for small volumes of low concentration CNC suspensions, a deeper understanding of the influence of the dispersing process is needed to work with larger volumes, at higher concentrations.
34534796	0	21	theme	cellulose	51:59	arg1	dispersions					73:83	sonicated cellulose nanocrystal dispersions	41:83	sonicated cellulose nanocrystal dispersions	41:83	Rheological insights on the evolution of sonicated cellulose nanocrystal dispersions.
34534796	3	22	theme	larger	567:572	arg1	volumes					574:580	larger volumes	567:580	larger volumes	567:580	While the optimized parameters for sonication are now well defined for small volumes of low concentration CNC suspensions, a deeper understanding of the influence of the dispersing process is needed to work with larger volumes, at higher concentrations.
34534796	3	23	theme	deeper	480:485	arg1	understanding					487:499	a deeper understanding	478:499	a deeper understanding of the influence of the dispersing process	478:542	While the optimized parameters for sonication are now well defined for small volumes of low concentration CNC suspensions, a deeper understanding of the influence of the dispersing process is needed to work with larger volumes, at higher concentrations.
34534796	0	24	theme	sonicated	41:49	arg1	dispersions					73:83	sonicated cellulose nanocrystal dispersions	41:83	sonicated cellulose nanocrystal dispersions	41:83	Rheological insights on the evolution of sonicated cellulose nanocrystal dispersions.
34534796	1	25	theme	promising	120:128	arg1	biomaterials					130:141	promising biomaterials	120:141	promising biomaterials	120:141	Cellulose nanocrystals (CNCs) are promising biomaterials, but their tendency to agglomerate when dried limits their use in several applications.
34534796	1	25	theme	promising	120:128	arg1	nanocrystals					96:107	Cellulose nanocrystals	86:107	Cellulose nanocrystals (CNCs)	86:114	Cellulose nanocrystals (CNCs) are promising biomaterials, but their tendency to agglomerate when dried limits their use in several applications.
34534796	5	26	theme	dispersion	956:965	arg1	state					967:971	the dispersion state	952:971	the dispersion state	952:971	After considering the importance of the measurement sampling volume, the behavior of a more concentrated suspension (6.4 wt%) is examined and compared with a never-dried suspension of the same concentration to validate the dispersion state.
34534796	4	27	dep	distribution	648:659	arg1	the					644:646	the	644:646	the	644:646	Herein, rheology is used to define the distribution and dispersion states upon ultrasonication of a 3.2 wt% CNC suspension.
34534796	1	28	from	use	202:204	arg1	applications					217:228	several applications	209:228	several applications	209:228	Cellulose nanocrystals (CNCs) are promising biomaterials, but their tendency to agglomerate when dried limits their use in several applications.
34534796	4	29	theme	3.2 wt	709:714	arg1	suspension					721:730	a 3.2 wt% CNC suspension	707:730	a 3.2 wt% CNC suspension	707:730	Herein, rheology is used to define the distribution and dispersion states upon ultrasonication of a 3.2 wt% CNC suspension.
34534796	2	30	from	CNCs	276:279	arg1	water					284:288	water	284:288	water	284:288	Ultrasonication is commonly used to disperse CNCs in water, bringing enough energy to the suspension to break agglomerates.
34534796	3	31	theme	dispersing	525:534	arg1	process					536:542	the dispersing process	521:542	the dispersing process	521:542	While the optimized parameters for sonication are now well defined for small volumes of low concentration CNC suspensions, a deeper understanding of the influence of the dispersing process is needed to work with larger volumes, at higher concentrations.
34534796	5	32	theme	concentration	926:938	arg1	suspension					903:912	a never-dried suspension	889:912	a never-dried suspension of the same concentration	889:938	After considering the importance of the measurement sampling volume, the behavior of a more concentrated suspension (6.4 wt%) is examined and compared with a never-dried suspension of the same concentration to validate the dispersion state.
34534796	5	33	theme	never-dried	891:901	arg1	suspension					903:912	a never-dried suspension	889:912	a never-dried suspension of the same concentration	889:938	After considering the importance of the measurement sampling volume, the behavior of a more concentrated suspension (6.4 wt%) is examined and compared with a never-dried suspension of the same concentration to validate the dispersion state.
34534796	0	34	theme	dispersions	73:83	arg1	evolution					28:36	the evolution	24:36	the evolution of sonicated cellulose nanocrystal dispersions	24:83	Rheological insights on the evolution of sonicated cellulose nanocrystal dispersions.
34534796	1	35	theme	several	209:215	arg1	applications					217:228	several applications	209:228	several applications	209:228	Cellulose nanocrystals (CNCs) are promising biomaterials, but their tendency to agglomerate when dried limits their use in several applications.
34534796	3	36	theme	higher	586:591	arg1	concentrations					593:606	higher concentrations	586:606	higher concentrations	586:606	While the optimized parameters for sonication are now well defined for small volumes of low concentration CNC suspensions, a deeper understanding of the influence of the dispersing process is needed to work with larger volumes, at higher concentrations.
34534796	4	37	used	used	629:632	arg2	rheology					617:624	rheology	617:624	rheology	617:624	Herein, rheology is used to define the distribution and dispersion states upon ultrasonication of a 3.2 wt% CNC suspension.
34534796	3	38	theme	influence	508:516	arg1	understanding					487:499	a deeper understanding	478:499	a deeper understanding of the influence of the dispersing process	478:542	While the optimized parameters for sonication are now well defined for small volumes of low concentration CNC suspensions, a deeper understanding of the influence of the dispersing process is needed to work with larger volumes, at higher concentrations.
34534796	0	39	theme	nanocrystal	61:71	arg1	dispersions					73:83	sonicated cellulose nanocrystal dispersions	41:83	sonicated cellulose nanocrystal dispersions	41:83	Rheological insights on the evolution of sonicated cellulose nanocrystal dispersions.
34445079	7	0	dep	crosslinked	1104:1114	arg1	surface					1096:1102	surface	1096:1102	surface	1096:1102	To improve their dimensional stability in aqueous medium, the CS_GO membranes were surface crosslinked with glutaraldehyde.
34445079	6	1	theme	different	927:935	arg1	GO					948:949	GO	948:949	GO	948:949	In this work, chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO were prepared and used as sorbents for the SPE of pesticides.
34445079	6	1	theme	different	927:935	arg1	amounts					937:943	different amounts	927:943	different amounts of GO	927:949	In this work, chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO were prepared and used as sorbents for the SPE of pesticides.
34445079	3	2	link	graphite-derived	438:453	arg1	materials					455:463	graphite-derived materials	438:463	graphite-derived materials	438:463	Recently, much attention has been paid to inorganic nanosystems such as graphite-derived materials.
34445079	1	3	from	determination	172:184	arg1	samples					230:236	liquid samples	223:236	liquid samples	223:236	Solid-phase extraction (SPE) coupled to LC/MS/MS analysis is a valid approach for the determination of organic micropollutants (OMPs) in liquid samples.
34445079	10	4	theme	different	1737:1745	arg1	pollutants					1771:1780	different hydrophilic/hydrophobic pollutants	1737:1780	different hydrophilic/hydrophobic pollutants	1737:1780	The increased dimensional stability in water, together with the presence of high GO amounts, made the prepared composite membranes more efficacious than the ones based just on CS in isolating and preconcentrating different hydrophilic/hydrophobic pollutants.
34445079	9	5	theme	properties	1434:1443	arg1	reduction					1452:1460	a reduction	1450:1460	a reduction of thermal stability of the CS-based membranes	1450:1507	As the GO content increased, a decrease in surface homogeneity, an improvement of mechanical properties, and a reduction of thermal stability of the CS-based membranes were observed.
34445079	9	5	theme	properties	1434:1443	arg1	decrease					1372:1379	a decrease	1370:1379	a decrease in surface homogeneity	1370:1402	As the GO content increased, a decrease in surface homogeneity, an improvement of mechanical properties, and a reduction of thermal stability of the CS-based membranes were observed.
34445079	9	5	theme	properties	1434:1443	arg1	improvement					1408:1418	an improvement	1405:1418	an improvement of mechanical properties	1405:1443	As the GO content increased, a decrease in surface homogeneity, an improvement of mechanical properties, and a reduction of thermal stability of the CS-based membranes were observed.
34445079	8	6	dep	Fourier	1181:1187	arg1	transform					1189:1197	transform	1189:1197	transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, swelling degree, contact angle, and mechanical measurements	1189:1338	The composite systems were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, swelling degree, contact angle, and mechanical measurements.
34445079	9	7	theme	stability	1473:1481	arg1	reduction					1452:1460	a reduction	1450:1460	a reduction of thermal stability of the CS-based membranes	1450:1507	As the GO content increased, a decrease in surface homogeneity, an improvement of mechanical properties, and a reduction of thermal stability of the CS-based membranes were observed.
34445079	9	7	theme	stability	1473:1481	arg1	decrease					1372:1379	a decrease	1370:1379	a decrease in surface homogeneity	1370:1402	As the GO content increased, a decrease in surface homogeneity, an improvement of mechanical properties, and a reduction of thermal stability of the CS-based membranes were observed.
34445079	9	7	theme	stability	1473:1481	arg1	improvement					1408:1418	an improvement	1405:1418	an improvement of mechanical properties	1405:1443	As the GO content increased, a decrease in surface homogeneity, an improvement of mechanical properties, and a reduction of thermal stability of the CS-based membranes were observed.
34445079	2	8	theme	materials	344:352	arg1	development					310:320	the development	306:320	the development of innovative sorbent materials	306:352	To remove the greatest number of OMPs from environmental matrices, the development of innovative sorbent materials is crucial.
34445079	2	8	theme	materials	344:352	arg1	crucial					357:363	crucial	357:363	crucial	357:363	To remove the greatest number of OMPs from environmental matrices, the development of innovative sorbent materials is crucial.
34445079	8	9	dep	transform	1189:1197	arg1	infrared					1199:1206	infrared	1199:1206	transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, swelling degree, contact angle, and mechanical measurements	1189:1338	The composite systems were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, swelling degree, contact angle, and mechanical measurements.
34445079	10	10	theme	high	1600:1603	arg1	amounts					1608:1614	high GO amounts	1600:1614	high GO amounts	1600:1614	The increased dimensional stability in water, together with the presence of high GO amounts, made the prepared composite membranes more efficacious than the ones based just on CS in isolating and preconcentrating different hydrophilic/hydrophobic pollutants.
34445079	10	11	theme	increased	1528:1536	arg1	stability					1550:1558	The increased dimensional stability	1524:1558	The increased dimensional stability in water	1524:1567	The increased dimensional stability in water, together with the presence of high GO amounts, made the prepared composite membranes more efficacious than the ones based just on CS in isolating and preconcentrating different hydrophilic/hydrophobic pollutants.
34445079	5	12	used	used	679:682	arg2	media					698:702	convenient media	687:702	convenient media for the dispersion of sorbent materials, thanks to their unique properties such as biodegradability, biocompatibility, nontoxicity, and low cost	687:847	Polysaccharides have also been widely used as convenient media for the dispersion of sorbent materials, thanks to their unique properties such as biodegradability, biocompatibility, nontoxicity, and low cost.
34445079	5	12	used	used	679:682	arg2	Polysaccharides					641:655	Polysaccharides	641:655	Polysaccharides	641:655	Polysaccharides have also been widely used as convenient media for the dispersion of sorbent materials, thanks to their unique properties such as biodegradability, biocompatibility, nontoxicity, and low cost.
34445079	6	13	theme	composite	896:904	arg1	membranes					906:914	chitosan-graphene oxide (CS_GO) composite membranes	864:914	chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO	864:949	In this work, chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO were prepared and used as sorbents for the SPE of pesticides.
34445079	6	13	theme	composite	896:904	arg1	sorbents					977:984	sorbents	977:984	sorbents	977:984	In this work, chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO were prepared and used as sorbents for the SPE of pesticides.
34445079	10	14	theme	prepared	1626:1633	arg1	membranes					1645:1653	the prepared composite membranes	1622:1653	the prepared composite membranes	1622:1653	The increased dimensional stability in water, together with the presence of high GO amounts, made the prepared composite membranes more efficacious than the ones based just on CS in isolating and preconcentrating different hydrophilic/hydrophobic pollutants.
34445079	2	15	theme	OMPs	272:275	arg1	number					262:267	the greatest number	249:267	the greatest number of OMPs from environmental matrices	249:303	To remove the greatest number of OMPs from environmental matrices, the development of innovative sorbent materials is crucial.
34445079	4	16	theme	flame	595:599	arg1	retardants					601:610	flame retardants	595:610	flame retardants	595:610	Graphene oxide has been employed in water-purification processes, including the removal of several micropollutants such as dyes, flame retardants, or pharmaceutical products.
34445079	8	17	theme	mechanical	1316:1325	arg1	measurements					1327:1338	mechanical measurements	1316:1338	mechanical measurements	1316:1338	The composite systems were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, swelling degree, contact angle, and mechanical measurements.
34445079	10	18	dep	amounts	1608:1614	arg1	presence					1588:1595	presence	1588:1595	presence	1588:1595	The increased dimensional stability in water, together with the presence of high GO amounts, made the prepared composite membranes more efficacious than the ones based just on CS in isolating and preconcentrating different hydrophilic/hydrophobic pollutants.
34445079	10	18	dep	amounts	1608:1614	arg1	GO					1605:1606	GO	1605:1606	GO	1605:1606	The increased dimensional stability in water, together with the presence of high GO amounts, made the prepared composite membranes more efficacious than the ones based just on CS in isolating and preconcentrating different hydrophilic/hydrophobic pollutants.
34445079	10	18	dep	amounts	1608:1614	arg1	the					1584:1586	the	1584:1586	the	1584:1586	The increased dimensional stability in water, together with the presence of high GO amounts, made the prepared composite membranes more efficacious than the ones based just on CS in isolating and preconcentrating different hydrophilic/hydrophobic pollutants.
34445079	1	19	theme	Solid-phase	86:96	arg1	SPE					110:112	SPE	110:112	SPE	110:112	Solid-phase extraction (SPE) coupled to LC/MS/MS analysis is a valid approach for the determination of organic micropollutants (OMPs) in liquid samples.
34445079	1	19	theme	Solid-phase	86:96	arg1	extraction					98:107	Solid-phase extraction	86:107	Solid-phase extraction (SPE) coupled to LC/MS/MS analysis	86:142	Solid-phase extraction (SPE) coupled to LC/MS/MS analysis is a valid approach for the determination of organic micropollutants (OMPs) in liquid samples.
34445079	1	19	theme	Solid-phase	86:96	arg1	approach					155:162	a valid approach	147:162	a valid approach for the determination of organic micropollutants (OMPs) in liquid samples	147:236	Solid-phase extraction (SPE) coupled to LC/MS/MS analysis is a valid approach for the determination of organic micropollutants (OMPs) in liquid samples.
34445079	9	20	theme	thermal	1465:1471	arg1	stability					1473:1481	thermal stability	1465:1481	thermal stability of the CS-based membranes	1465:1507	As the GO content increased, a decrease in surface homogeneity, an improvement of mechanical properties, and a reduction of thermal stability of the CS-based membranes were observed.
34445079	10	21	from	stability	1550:1558	arg1	water					1563:1567	water	1563:1567	water	1563:1567	The increased dimensional stability in water, together with the presence of high GO amounts, made the prepared composite membranes more efficacious than the ones based just on CS in isolating and preconcentrating different hydrophilic/hydrophobic pollutants.
34445079	9	22	from	decrease	1372:1379	arg1	homogeneity					1392:1402	surface homogeneity	1384:1402	surface homogeneity	1384:1402	As the GO content increased, a decrease in surface homogeneity, an improvement of mechanical properties, and a reduction of thermal stability of the CS-based membranes were observed.
34445079	6	23	contain	containing	916:925	arg2	amounts					937:943	different amounts	927:943	different amounts of GO	927:949	In this work, chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO were prepared and used as sorbents for the SPE of pesticides.
34445079	6	23	contain	containing	916:925	arg2	GO					948:949	GO	948:949	GO	948:949	In this work, chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO were prepared and used as sorbents for the SPE of pesticides.
34445079	6	23	contain	containing	916:925	arg1	membranes					906:914	chitosan-graphene oxide (CS_GO) composite membranes	864:914	chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO	864:949	In this work, chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO were prepared and used as sorbents for the SPE of pesticides.
34445079	6	23	contain	containing	916:925	arg1	sorbents					977:984	sorbents	977:984	sorbents	977:984	In this work, chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO were prepared and used as sorbents for the SPE of pesticides.
34445079	0	24	theme	Oxide	18:22	arg1	Composite					24:32	Chitosan-Graphene Oxide Composite	0:32	Chitosan-Graphene Oxide Composite	0:32	Chitosan-Graphene Oxide Composite Membranes for Solid-Phase Extraction of Pesticides.
34445079	7	25	theme	CS_GO	1075:1079	arg1	membranes					1081:1089	the CS_GO membranes	1071:1089	the CS_GO membranes	1071:1089	To improve their dimensional stability in aqueous medium, the CS_GO membranes were surface crosslinked with glutaraldehyde.
34445079	8	26	theme	composite	1141:1149	arg1	systems					1151:1157	The composite systems	1137:1157	The composite systems	1137:1157	The composite systems were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, swelling degree, contact angle, and mechanical measurements.
34445079	3	27	theme	inorganic	408:416	arg1	materials					455:463	graphite-derived materials	438:463	graphite-derived materials	438:463	Recently, much attention has been paid to inorganic nanosystems such as graphite-derived materials.
34445079	3	27	theme	inorganic	408:416	arg1	nanosystems					418:428	inorganic nanosystems	408:428	inorganic nanosystems such as graphite-derived materials	408:463	Recently, much attention has been paid to inorganic nanosystems such as graphite-derived materials.
34445079	0	28	theme	Chitosan-Graphene	0:16	arg1	Composite					24:32	Chitosan-Graphene Oxide Composite	0:32	Chitosan-Graphene Oxide Composite	0:32	Chitosan-Graphene Oxide Composite Membranes for Solid-Phase Extraction of Pesticides.
34445079	4	29	theme	micropollutants	565:579	arg1	removal					546:552	the removal	542:552	the removal of several micropollutants such as dyes, flame retardants, or pharmaceutical products	542:638	Graphene oxide has been employed in water-purification processes, including the removal of several micropollutants such as dyes, flame retardants, or pharmaceutical products.
34445079	1	30	theme	organic	189:195	arg1	OMPs					214:217	OMPs	214:217	OMPs	214:217	Solid-phase extraction (SPE) coupled to LC/MS/MS analysis is a valid approach for the determination of organic micropollutants (OMPs) in liquid samples.
34445079	1	30	theme	organic	189:195	arg1	micropollutants					197:211	organic micropollutants	189:211	organic micropollutants (OMPs)	189:218	Solid-phase extraction (SPE) coupled to LC/MS/MS analysis is a valid approach for the determination of organic micropollutants (OMPs) in liquid samples.
34445079	8	31	theme	scanning	1222:1229	arg1	microscopy					1240:1249	scanning electron microscopy	1222:1249	scanning electron microscopy	1222:1249	The composite systems were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, swelling degree, contact angle, and mechanical measurements.
34445079	4	32	theme	several	557:563	arg1	micropollutants					565:579	several micropollutants	557:579	several micropollutants such as dyes, flame retardants, or pharmaceutical products	557:638	Graphene oxide has been employed in water-purification processes, including the removal of several micropollutants such as dyes, flame retardants, or pharmaceutical products.
34445079	4	32	theme	several	557:563	arg1	dyes					589:592	dyes	589:592	dyes	589:592	Graphene oxide has been employed in water-purification processes, including the removal of several micropollutants such as dyes, flame retardants, or pharmaceutical products.
34445079	4	32	theme	several	557:563	arg1	retardants					601:610	flame retardants	595:610	flame retardants	595:610	Graphene oxide has been employed in water-purification processes, including the removal of several micropollutants such as dyes, flame retardants, or pharmaceutical products.
34445079	4	32	theme	several	557:563	arg1	products					631:638	pharmaceutical products	616:638	pharmaceutical products	616:638	Graphene oxide has been employed in water-purification processes, including the removal of several micropollutants such as dyes, flame retardants, or pharmaceutical products.
34445079	1	33	theme	micropollutants	197:211	arg1	determination					172:184	the determination	168:184	the determination of organic micropollutants (OMPs) in liquid samples	168:236	Solid-phase extraction (SPE) coupled to LC/MS/MS analysis is a valid approach for the determination of organic micropollutants (OMPs) in liquid samples.
34445079	5	34	theme	low	840:842	arg1	cost					844:847	low cost	840:847	low cost	840:847	Polysaccharides have also been widely used as convenient media for the dispersion of sorbent materials, thanks to their unique properties such as biodegradability, biocompatibility, nontoxicity, and low cost.
34445079	7	35	theme	aqueous	1055:1061	arg1	medium					1063:1068	aqueous medium	1055:1068	aqueous medium	1055:1068	To improve their dimensional stability in aqueous medium, the CS_GO membranes were surface crosslinked with glutaraldehyde.
34445079	6	36	theme	oxide	882:886	arg1	membranes					906:914	chitosan-graphene oxide (CS_GO) composite membranes	864:914	chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO	864:949	In this work, chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO were prepared and used as sorbents for the SPE of pesticides.
34445079	6	36	theme	oxide	882:886	arg1	sorbents					977:984	sorbents	977:984	sorbents	977:984	In this work, chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO were prepared and used as sorbents for the SPE of pesticides.
34445079	2	37	theme	environmental	282:294	arg1	matrices					296:303	environmental matrices	282:303	environmental matrices	282:303	To remove the greatest number of OMPs from environmental matrices, the development of innovative sorbent materials is crucial.
34445079	0	38	theme	Solid-Phase	48:58	arg1	Extraction					60:69	Solid-Phase Extraction	48:69	Solid-Phase Extraction of Pesticides	48:83	Chitosan-Graphene Oxide Composite Membranes for Solid-Phase Extraction of Pesticides.
34445079	2	39	theme	innovative	325:334	arg1	materials					344:352	innovative sorbent materials	325:352	innovative sorbent materials	325:352	To remove the greatest number of OMPs from environmental matrices, the development of innovative sorbent materials is crucial.
34445079	5	40	theme	sorbent	726:732	arg1	materials					734:742	sorbent materials	726:742	sorbent materials	726:742	Polysaccharides have also been widely used as convenient media for the dispersion of sorbent materials, thanks to their unique properties such as biodegradability, biocompatibility, nontoxicity, and low cost.
34445079	6	41	theme	chitosan-graphene	864:880	arg1	CS_GO					889:893	CS_GO	889:893	CS_GO	889:893	In this work, chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO were prepared and used as sorbents for the SPE of pesticides.
34445079	6	41	theme	chitosan-graphene	864:880	arg1	oxide					882:886	chitosan-graphene oxide	864:886	chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO	864:949	In this work, chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO were prepared and used as sorbents for the SPE of pesticides.
34445079	9	42	theme	mechanical	1423:1432	arg1	properties					1434:1443	mechanical properties	1423:1443	mechanical properties	1423:1443	As the GO content increased, a decrease in surface homogeneity, an improvement of mechanical properties, and a reduction of thermal stability of the CS-based membranes were observed.
34445079	8	43	theme	swelling	1280:1287	arg1	degree					1289:1294	swelling degree	1280:1294	swelling degree	1280:1294	The composite systems were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, swelling degree, contact angle, and mechanical measurements.
34445079	3	44	theme	graphite-derived	438:453	arg1	materials					455:463	graphite-derived materials	438:463	graphite-derived materials	438:463	Recently, much attention has been paid to inorganic nanosystems such as graphite-derived materials.
34445079	8	45	theme	electron	1231:1238	arg1	microscopy					1240:1249	scanning electron microscopy	1222:1249	scanning electron microscopy	1222:1249	The composite systems were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, swelling degree, contact angle, and mechanical measurements.
34445079	5	46	theme	materials	734:742	arg1	dispersion					712:721	the dispersion	708:721	the dispersion of sorbent materials, thanks to their unique properties such as biodegradability, biocompatibility, nontoxicity, and low cost	708:847	Polysaccharides have also been widely used as convenient media for the dispersion of sorbent materials, thanks to their unique properties such as biodegradability, biocompatibility, nontoxicity, and low cost.
34445079	4	47	theme	Graphene	466:473	arg1	oxide					475:479	Graphene oxide	466:479	Graphene oxide	466:479	Graphene oxide has been employed in water-purification processes, including the removal of several micropollutants such as dyes, flame retardants, or pharmaceutical products.
34445079	9	48	theme	membranes	1499:1507	arg1	stability					1473:1481	thermal stability	1465:1481	thermal stability of the CS-based membranes	1465:1507	As the GO content increased, a decrease in surface homogeneity, an improvement of mechanical properties, and a reduction of thermal stability of the CS-based membranes were observed.
34445079	8	49	theme	thermogravimetric	1252:1268	arg1	analysis					1270:1277	thermogravimetric analysis	1252:1277	thermogravimetric analysis	1252:1277	The composite systems were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, swelling degree, contact angle, and mechanical measurements.
34445079	9	50	theme	surface	1384:1390	arg1	homogeneity					1392:1402	surface homogeneity	1384:1402	surface homogeneity	1384:1402	As the GO content increased, a decrease in surface homogeneity, an improvement of mechanical properties, and a reduction of thermal stability of the CS-based membranes were observed.
34445079	5	51	theme	convenient	687:696	arg1	media					698:702	convenient media	687:702	convenient media for the dispersion of sorbent materials, thanks to their unique properties such as biodegradability, biocompatibility, nontoxicity, and low cost	687:847	Polysaccharides have also been widely used as convenient media for the dispersion of sorbent materials, thanks to their unique properties such as biodegradability, biocompatibility, nontoxicity, and low cost.
34445079	5	51	theme	convenient	687:696	arg1	Polysaccharides					641:655	Polysaccharides	641:655	Polysaccharides	641:655	Polysaccharides have also been widely used as convenient media for the dispersion of sorbent materials, thanks to their unique properties such as biodegradability, biocompatibility, nontoxicity, and low cost.
34445079	9	52	theme	CS-based	1490:1497	arg1	membranes					1499:1507	the CS-based membranes	1486:1507	the CS-based membranes	1486:1507	As the GO content increased, a decrease in surface homogeneity, an improvement of mechanical properties, and a reduction of thermal stability of the CS-based membranes were observed.
34445079	2	53	theme	sorbent	336:342	arg1	materials					344:352	innovative sorbent materials	325:352	innovative sorbent materials	325:352	To remove the greatest number of OMPs from environmental matrices, the development of innovative sorbent materials is crucial.
34445079	10	54	theme	hydrophilic/hydrophobic	1747:1769	arg1	pollutants					1771:1780	different hydrophilic/hydrophobic pollutants	1737:1780	different hydrophilic/hydrophobic pollutants	1737:1780	The increased dimensional stability in water, together with the presence of high GO amounts, made the prepared composite membranes more efficacious than the ones based just on CS in isolating and preconcentrating different hydrophilic/hydrophobic pollutants.
34445079	6	55	theme	GO	948:949	arg1	GO					948:949	GO	948:949	GO	948:949	In this work, chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO were prepared and used as sorbents for the SPE of pesticides.
34445079	6	55	theme	GO	948:949	arg1	amounts					937:943	different amounts	927:943	different amounts of GO	927:949	In this work, chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO were prepared and used as sorbents for the SPE of pesticides.
34445079	6	56	used	used	969:972	arg2	sorbents					977:984	sorbents	977:984	sorbents	977:984	In this work, chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO were prepared and used as sorbents for the SPE of pesticides.
34445079	6	56	used	used	969:972	arg2	membranes					906:914	chitosan-graphene oxide (CS_GO) composite membranes	864:914	chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO	864:949	In this work, chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO were prepared and used as sorbents for the SPE of pesticides.
34445079	1	57	theme	LC/MS/MS	126:133	arg1	analysis					135:142	LC/MS/MS analysis	126:142	LC/MS/MS analysis	126:142	Solid-phase extraction (SPE) coupled to LC/MS/MS analysis is a valid approach for the determination of organic micropollutants (OMPs) in liquid samples.
34445079	9	58	dep	content	1351:1357	arg1	GO					1348:1349	GO	1348:1349	GO	1348:1349	As the GO content increased, a decrease in surface homogeneity, an improvement of mechanical properties, and a reduction of thermal stability of the CS-based membranes were observed.
34445079	10	59	theme	dimensional	1538:1548	arg1	stability					1550:1558	The increased dimensional stability	1524:1558	The increased dimensional stability in water	1524:1567	The increased dimensional stability in water, together with the presence of high GO amounts, made the prepared composite membranes more efficacious than the ones based just on CS in isolating and preconcentrating different hydrophilic/hydrophobic pollutants.
34445079	2	60	theme	greatest	253:260	arg1	number					262:267	the greatest number	249:267	the greatest number of OMPs from environmental matrices	249:303	To remove the greatest number of OMPs from environmental matrices, the development of innovative sorbent materials is crucial.
34445079	2	61	from	number	262:267	arg1	matrices					296:303	environmental matrices	282:303	environmental matrices	282:303	To remove the greatest number of OMPs from environmental matrices, the development of innovative sorbent materials is crucial.
34445079	7	62	theme	dimensional	1030:1040	arg1	stability					1042:1050	their dimensional stability	1024:1050	their dimensional stability	1024:1050	To improve their dimensional stability in aqueous medium, the CS_GO membranes were surface crosslinked with glutaraldehyde.
34445079	9	63	from	improvement	1408:1418	arg1	homogeneity					1392:1402	surface homogeneity	1384:1402	surface homogeneity	1384:1402	As the GO content increased, a decrease in surface homogeneity, an improvement of mechanical properties, and a reduction of thermal stability of the CS-based membranes were observed.
34445079	10	64	theme	composite	1635:1643	arg1	membranes					1645:1653	the prepared composite membranes	1622:1653	the prepared composite membranes	1622:1653	The increased dimensional stability in water, together with the presence of high GO amounts, made the prepared composite membranes more efficacious than the ones based just on CS in isolating and preconcentrating different hydrophilic/hydrophobic pollutants.
34445079	5	65	theme	thanks	745:750	arg1	materials					734:742	sorbent materials	726:742	sorbent materials	726:742	Polysaccharides have also been widely used as convenient media for the dispersion of sorbent materials, thanks to their unique properties such as biodegradability, biocompatibility, nontoxicity, and low cost.
34445079	1	66	theme	liquid	223:228	arg1	samples					230:236	liquid samples	223:236	liquid samples	223:236	Solid-phase extraction (SPE) coupled to LC/MS/MS analysis is a valid approach for the determination of organic micropollutants (OMPs) in liquid samples.
34445079	5	67	theme	unique	761:766	arg1	biocompatibility					805:820	biocompatibility	805:820	biocompatibility	805:820	Polysaccharides have also been widely used as convenient media for the dispersion of sorbent materials, thanks to their unique properties such as biodegradability, biocompatibility, nontoxicity, and low cost.
34445079	5	67	theme	unique	761:766	arg1	properties					768:777	their unique properties	755:777	their unique properties such as biodegradability, biocompatibility, nontoxicity, and low cost	755:847	Polysaccharides have also been widely used as convenient media for the dispersion of sorbent materials, thanks to their unique properties such as biodegradability, biocompatibility, nontoxicity, and low cost.
34445079	5	67	theme	unique	761:766	arg1	cost					844:847	low cost	840:847	low cost	840:847	Polysaccharides have also been widely used as convenient media for the dispersion of sorbent materials, thanks to their unique properties such as biodegradability, biocompatibility, nontoxicity, and low cost.
34445079	5	67	theme	unique	761:766	arg1	biodegradability					787:802	biodegradability	787:802	biodegradability	787:802	Polysaccharides have also been widely used as convenient media for the dispersion of sorbent materials, thanks to their unique properties such as biodegradability, biocompatibility, nontoxicity, and low cost.
34445079	5	67	theme	unique	761:766	arg1	nontoxicity					823:833	nontoxicity	823:833	nontoxicity	823:833	Polysaccharides have also been widely used as convenient media for the dispersion of sorbent materials, thanks to their unique properties such as biodegradability, biocompatibility, nontoxicity, and low cost.
34445079	8	68	theme	contact	1297:1303	arg1	angle					1305:1309	contact angle	1297:1309	contact angle	1297:1309	The composite systems were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, swelling degree, contact angle, and mechanical measurements.
34445079	3	69	theme	much	376:379	arg1	attention					381:389	much attention	376:389	much attention	376:389	Recently, much attention has been paid to inorganic nanosystems such as graphite-derived materials.
34445079	0	70	theme	Pesticides	74:83	arg1	Extraction					60:69	Solid-Phase Extraction	48:69	Solid-Phase Extraction of Pesticides	48:83	Chitosan-Graphene Oxide Composite Membranes for Solid-Phase Extraction of Pesticides.
34445079	4	71	theme	water-purification	502:519	arg1	removal					546:552	the removal	542:552	the removal of several micropollutants such as dyes, flame retardants, or pharmaceutical products	542:638	Graphene oxide has been employed in water-purification processes, including the removal of several micropollutants such as dyes, flame retardants, or pharmaceutical products.
34445079	4	71	theme	water-purification	502:519	arg1	processes					521:529	water-purification processes	502:529	water-purification processes	502:529	Graphene oxide has been employed in water-purification processes, including the removal of several micropollutants such as dyes, flame retardants, or pharmaceutical products.
34445079	6	72	theme	pesticides	1001:1010	arg1	SPE					994:996	the SPE	990:996	the SPE of pesticides	990:1010	In this work, chitosan-graphene oxide (CS_GO) composite membranes containing different amounts of GO were prepared and used as sorbents for the SPE of pesticides.
34445079	9	73	from	reduction	1452:1460	arg1	homogeneity					1392:1402	surface homogeneity	1384:1402	surface homogeneity	1384:1402	As the GO content increased, a decrease in surface homogeneity, an improvement of mechanical properties, and a reduction of thermal stability of the CS-based membranes were observed.
34445079	1	74	theme	valid	149:153	arg1	extraction					98:107	Solid-phase extraction	86:107	Solid-phase extraction (SPE) coupled to LC/MS/MS analysis	86:142	Solid-phase extraction (SPE) coupled to LC/MS/MS analysis is a valid approach for the determination of organic micropollutants (OMPs) in liquid samples.
34445079	1	74	theme	valid	149:153	arg1	approach					155:162	a valid approach	147:162	a valid approach for the determination of organic micropollutants (OMPs) in liquid samples	147:236	Solid-phase extraction (SPE) coupled to LC/MS/MS analysis is a valid approach for the determination of organic micropollutants (OMPs) in liquid samples.
34445079	2	75	from	matrices	296:303	arg1	OMPs					272:275	OMPs	272:275	OMPs from environmental matrices	272:303	To remove the greatest number of OMPs from environmental matrices, the development of innovative sorbent materials is crucial.
34445079	2	75	from	matrices	296:303	arg1	number					262:267	the greatest number	249:267	the greatest number of OMPs from environmental matrices	249:303	To remove the greatest number of OMPs from environmental matrices, the development of innovative sorbent materials is crucial.
34445079	4	76	theme	pharmaceutical	616:629	arg1	products					631:638	pharmaceutical products	616:638	pharmaceutical products	616:638	Graphene oxide has been employed in water-purification processes, including the removal of several micropollutants such as dyes, flame retardants, or pharmaceutical products.
34600883	2	0	theme	photocatalysis	602:615	arg1	functions					574:582	dual functions	569:582	dual functions of adsorption and photocatalysis	569:615	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	8	1	theme	PMS	1469:1471	arg1	activation					1473:1482	PMS activation	1469:1482	PMS activation	1469:1482	The proposed BOP-24 system used adsorption and a unique cavity structure to enrich AO7 near the active site, thereby reducing the path for PMS activation.
34600883	4	2	theme	%	834:834	arg1	system					868:873	the 24% PBCD-B-D (BOP-24)/visible light system	828:873	the 24% PBCD-B-D (BOP-24)/visible light system	828:873	Compared to the 24% PBCD-B-D (BOP-24)/visible light system, the degradation efficiency of BOP-24/PMS system for AO7 is increased from 64.1% to 99.2% within shorter time (∼60 min).
34600883	2	3	theme	Acid	662:665	arg1	AO7					677:679	AO7	677:679	AO7	677:679	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	2	3	theme	Acid	662:665	arg1	Orange					667:672	Acid Orange 7	662:674	Acid Orange 7 (AO7)	662:680	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	2	4	theme	adsorption	587:596	arg1	functions					574:582	dual functions	569:582	dual functions of adsorption and photocatalysis	569:615	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	6	5	theme	NO3-	1111:1114	arg1	addition					1083:1090	The addition	1079:1090	The addition of Cl-, SO42-, and NO3-	1079:1114	The addition of Cl-, SO42-, and NO3- promoted the photodegradation of AO7, whereas the addition of CO32- did not.
34600883	6	6	theme	AO7	1149:1151	arg1	photodegradation					1129:1144	the photodegradation	1125:1144	the photodegradation of AO7	1125:1151	The addition of Cl-, SO42-, and NO3- promoted the photodegradation of AO7, whereas the addition of CO32- did not.
34600883	3	7	theme	BiOBr/PBCD-B-D	711:724	arg1	combination					696:706	The combination	692:706	The combination of BiOBr/PBCD-B-D (BOP-24) with peroxymonosulfate (PMS)	692:762	The combination of BiOBr/PBCD-B-D (BOP-24) with peroxymonosulfate (PMS) showed an optimal adsorption-photocatalytic effect.
34600883	1	8	contain	has	326:328	arg2	advantages					334:343	the advantages	330:343	the advantages of a high degradation rate, affordability, and an absence of secondary pollution	330:424	The combination of adsorption-photocatalysis and advanced oxidation processes (AOP) based on sulfate (SO4•-) for the treatment of organic pollution has the advantages of a high degradation rate, affordability, and an absence of secondary pollution.
34600883	1	8	contain	has	326:328	arg1	combination					182:192	The combination	178:192	The combination of adsorption-photocatalysis and advanced oxidation processes (AOP) based on sulfate (SO4•-) for the treatment of organic pollution	178:324	The combination of adsorption-photocatalysis and advanced oxidation processes (AOP) based on sulfate (SO4•-) for the treatment of organic pollution has the advantages of a high degradation rate, affordability, and an absence of secondary pollution.
34600883	2	9	theme	composite	545:553	arg1	material					555:562	a composite material	543:562	a composite material with dual functions of adsorption and photocatalysis	543:615	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	1	10	theme	oxidation	236:244	arg1	processes					246:254	advanced oxidation processes	227:254	advanced oxidation processes	227:254	The combination of adsorption-photocatalysis and advanced oxidation processes (AOP) based on sulfate (SO4•-) for the treatment of organic pollution has the advantages of a high degradation rate, affordability, and an absence of secondary pollution.
34600883	4	11	theme	degradation	880:890	arg1	efficiency					892:901	the degradation efficiency	876:901	the degradation efficiency of BOP-24/PMS system for AO7	876:930	Compared to the 24% PBCD-B-D (BOP-24)/visible light system, the degradation efficiency of BOP-24/PMS system for AO7 is increased from 64.1% to 99.2% within shorter time (∼60 min).
34600883	7	12	theme	system	1264:1269	arg1	experiments					1218:1228	The free radical capture experiments	1193:1228	The free radical capture experiments of the BOP-24/PMS AO7 degradation system	1193:1269	The free radical capture experiments of the BOP-24/PMS AO7 degradation system showed that •O2-, h+, •OH, and SO4•- are reactive species.
34600883	0	13	theme	dye	153:155	arg1	pollutants					157:166	dye pollutants	153:166	dye pollutants	153:166	Insights into the photocatalytic activation of peroxymonosulfate by visible light over BiOBr-cyclodextrin polymer complexes for efficient degradation of dye pollutants in water.
34600883	7	14	theme	AO7	1248:1250	arg1	system					1264:1269	the BOP-24/PMS AO7 degradation system	1233:1269	the BOP-24/PMS AO7 degradation system	1233:1269	The free radical capture experiments of the BOP-24/PMS AO7 degradation system showed that •O2-, h+, •OH, and SO4•- are reactive species.
34600883	4	15	theme	light	862:866	arg1	system					868:873	the 24% PBCD-B-D (BOP-24)/visible light system	828:873	the 24% PBCD-B-D (BOP-24)/visible light system	828:873	Compared to the 24% PBCD-B-D (BOP-24)/visible light system, the degradation efficiency of BOP-24/PMS system for AO7 is increased from 64.1% to 99.2% within shorter time (∼60 min).
34600883	6	16	theme	CO32-	1178:1182	arg1	addition					1166:1173	the addition	1162:1173	the addition of CO32-	1162:1182	The addition of Cl-, SO42-, and NO3- promoted the photodegradation of AO7, whereas the addition of CO32- did not.
34600883	1	17	theme	pollution	416:424	arg1	rate					367:370	a high degradation rate	348:370	a high degradation rate	348:370	The combination of adsorption-photocatalysis and advanced oxidation processes (AOP) based on sulfate (SO4•-) for the treatment of organic pollution has the advantages of a high degradation rate, affordability, and an absence of secondary pollution.
34600883	1	17	theme	pollution	416:424	arg1	absence					395:401	an absence	392:401	an absence of secondary pollution	392:424	The combination of adsorption-photocatalysis and advanced oxidation processes (AOP) based on sulfate (SO4•-) for the treatment of organic pollution has the advantages of a high degradation rate, affordability, and an absence of secondary pollution.
34600883	1	17	theme	pollution	416:424	arg1	affordability					373:385	affordability	373:385	affordability	373:385	The combination of adsorption-photocatalysis and advanced oxidation processes (AOP) based on sulfate (SO4•-) for the treatment of organic pollution has the advantages of a high degradation rate, affordability, and an absence of secondary pollution.
34600883	8	18	theme	proposed	1334:1341	arg1	system					1350:1355	The proposed BOP-24 system	1330:1355	The proposed BOP-24 system	1330:1355	The proposed BOP-24 system used adsorption and a unique cavity structure to enrich AO7 near the active site, thereby reducing the path for PMS activation.
34600883	0	19	theme	visible	68:74	arg1	light					76:80	visible light	68:80	visible light over BiOBr-cyclodextrin polymer complexes for efficient degradation of dye pollutants in water	68:175	Insights into the photocatalytic activation of peroxymonosulfate by visible light over BiOBr-cyclodextrin polymer complexes for efficient degradation of dye pollutants in water.
34600883	5	20	theme	wide	1037:1040	arg1	range					1042:1046	a wide range	1035:1046	a wide range of pH application (pH = 3-11)	1035:1076	Moreover, the BOP-24/PMS system showed a wide range of pH application (pH = 3-11).
34600883	2	21	with	resin	497:501	arg1	functions					574:582	dual functions	569:582	dual functions of adsorption and photocatalysis	569:615	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	4	22	theme	/visible	853:860	arg1	system					868:873	the 24% PBCD-B-D (BOP-24)/visible light system	828:873	the 24% PBCD-B-D (BOP-24)/visible light system	828:873	Compared to the 24% PBCD-B-D (BOP-24)/visible light system, the degradation efficiency of BOP-24/PMS system for AO7 is increased from 64.1% to 99.2% within shorter time (∼60 min).
34600883	3	23	theme	adsorption-photocatalytic	782:806	arg1	effect					808:813	an optimal adsorption-photocatalytic effect	771:813	an optimal adsorption-photocatalytic effect	771:813	The combination of BiOBr/PBCD-B-D (BOP-24) with peroxymonosulfate (PMS) showed an optimal adsorption-photocatalytic effect.
34600883	6	24	theme	SO42-	1100:1104	arg1	addition					1083:1090	The addition	1079:1090	The addition of Cl-, SO42-, and NO3-	1079:1114	The addition of Cl-, SO42-, and NO3- promoted the photodegradation of AO7, whereas the addition of CO32- did not.
34600883	0	25	theme	polymer	106:112	arg1	complexes					114:122	BiOBr-cyclodextrin polymer complexes	87:122	BiOBr-cyclodextrin polymer complexes for efficient degradation of dye pollutants in water	87:175	Insights into the photocatalytic activation of peroxymonosulfate by visible light over BiOBr-cyclodextrin polymer complexes for efficient degradation of dye pollutants in water.
34600883	5	26	theme	BOP-24/PMS	1010:1019	arg1	system					1021:1026	the BOP-24/PMS system	1006:1026	the BOP-24/PMS system	1006:1026	Moreover, the BOP-24/PMS system showed a wide range of pH application (pH = 3-11).
34600883	4	27	theme	system	917:922	arg1	efficiency					892:901	the degradation efficiency	876:901	the degradation efficiency of BOP-24/PMS system for AO7	876:930	Compared to the 24% PBCD-B-D (BOP-24)/visible light system, the degradation efficiency of BOP-24/PMS system for AO7 is increased from 64.1% to 99.2% within shorter time (∼60 min).
34600883	2	28	with	oxybromide	523:532	arg1	functions					574:582	dual functions	569:582	dual functions of adsorption and photocatalysis	569:615	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	7	29	theme	capture	1210:1216	arg1	experiments					1218:1228	The free radical capture experiments	1193:1228	The free radical capture experiments of the BOP-24/PMS AO7 degradation system	1193:1269	The free radical capture experiments of the BOP-24/PMS AO7 degradation system showed that •O2-, h+, •OH, and SO4•- are reactive species.
34600883	2	30	theme	amphiphilic	447:457	arg1	PBCD-B-D					504:511	PBCD-B-D	504:511	PBCD-B-D	504:511	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	2	30	theme	amphiphilic	447:457	arg1	resin					497:501	amphiphilic super-crosslinked porous cyclodextrin resin	447:501	amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D)	447:512	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	8	31	theme	cavity	1386:1391	arg1	structure					1393:1401	a unique cavity structure	1377:1401	a unique cavity structure	1377:1401	The proposed BOP-24 system used adsorption and a unique cavity structure to enrich AO7 near the active site, thereby reducing the path for PMS activation.
34600883	2	32	link	super-crosslinked	459:475	arg1	PBCD-B-D					504:511	PBCD-B-D	504:511	PBCD-B-D	504:511	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	2	32	link	super-crosslinked	459:475	arg1	resin					497:501	amphiphilic super-crosslinked porous cyclodextrin resin	447:501	amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D)	447:512	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	7	33	theme	free	1197:1200	arg1	experiments					1218:1228	The free radical capture experiments	1193:1228	The free radical capture experiments of the BOP-24/PMS AO7 degradation system	1193:1269	The free radical capture experiments of the BOP-24/PMS AO7 degradation system showed that •O2-, h+, •OH, and SO4•- are reactive species.
34600883	4	34	theme	shorter	972:978	arg1	∼60 min					986:992	∼60 min	986:992	∼60 min	986:992	Compared to the 24% PBCD-B-D (BOP-24)/visible light system, the degradation efficiency of BOP-24/PMS system for AO7 is increased from 64.1% to 99.2% within shorter time (∼60 min).
34600883	4	34	theme	shorter	972:978	arg1	time					980:983	shorter time	972:983	shorter time (∼60 min)	972:993	Compared to the 24% PBCD-B-D (BOP-24)/visible light system, the degradation efficiency of BOP-24/PMS system for AO7 is increased from 64.1% to 99.2% within shorter time (∼60 min).
34600883	9	35	theme	separation	1627:1636	arg1	efficiency					1605:1614	the efficiency	1601:1614	the efficiency of carrier separation	1601:1636	PMS also acted as an electron (e-) acceptor to promote the transfer of part of e- to PMS, thereby further improving the efficiency of carrier separation.
34600883	1	36	theme	organic	308:314	arg1	pollution					316:324	organic pollution	308:324	organic pollution	308:324	The combination of adsorption-photocatalysis and advanced oxidation processes (AOP) based on sulfate (SO4•-) for the treatment of organic pollution has the advantages of a high degradation rate, affordability, and an absence of secondary pollution.
34600883	2	37	theme	porous	477:482	arg1	PBCD-B-D					504:511	PBCD-B-D	504:511	PBCD-B-D	504:511	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	2	37	theme	porous	477:482	arg1	resin					497:501	amphiphilic super-crosslinked porous cyclodextrin resin	447:501	amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D)	447:512	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	5	38	theme	application	1054:1064	arg1	range					1042:1046	a wide range	1035:1046	a wide range of pH application (pH = 3-11)	1035:1076	Moreover, the BOP-24/PMS system showed a wide range of pH application (pH = 3-11).
34600883	0	39	theme	peroxymonosulfate	47:63	arg1	activation					33:42	the photocatalytic activation	14:42	the photocatalytic activation of peroxymonosulfate by visible light over BiOBr-cyclodextrin polymer complexes for efficient degradation of dye pollutants in water	14:175	Insights into the photocatalytic activation of peroxymonosulfate by visible light over BiOBr-cyclodextrin polymer complexes for efficient degradation of dye pollutants in water.
34600883	0	40	from	degradation	138:148	arg1	water					171:175	water	171:175	water	171:175	Insights into the photocatalytic activation of peroxymonosulfate by visible light over BiOBr-cyclodextrin polymer complexes for efficient degradation of dye pollutants in water.
34600883	10	41	theme	pollutants	1712:1721	arg1	degradation					1697:1707	the degradation	1693:1707	the degradation of pollutants	1693:1721	The proposed system is an effective method to improve the degradation of pollutants and broadens the range of application of SO4•--based AOP technology.
34600883	2	42	with	material	555:562	arg1	functions					574:582	dual functions	569:582	dual functions of adsorption and photocatalysis	569:615	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	4	43	theme	24	832:833	arg1	%					834:834	%	834:834	%	834:834	Compared to the 24% PBCD-B-D (BOP-24)/visible light system, the degradation efficiency of BOP-24/PMS system for AO7 is increased from 64.1% to 99.2% within shorter time (∼60 min).
34600883	7	44	theme	reactive	1312:1319	arg1	•OH					1293:1295	•OH	1293:1295	•OH	1293:1295	The free radical capture experiments of the BOP-24/PMS AO7 degradation system showed that •O2-, h+, •OH, and SO4•- are reactive species.
34600883	7	44	theme	reactive	1312:1319	arg1	SO4•-					1302:1306	SO4•-	1302:1306	SO4•-	1302:1306	The free radical capture experiments of the BOP-24/PMS AO7 degradation system showed that •O2-, h+, •OH, and SO4•- are reactive species.
34600883	7	44	theme	reactive	1312:1319	arg1	species					1321:1327	reactive species	1312:1327	reactive species	1312:1327	The free radical capture experiments of the BOP-24/PMS AO7 degradation system showed that •O2-, h+, •OH, and SO4•- are reactive species.
34600883	7	44	theme	reactive	1312:1319	arg1	h+					1289:1290	h+	1289:1290	h+	1289:1290	The free radical capture experiments of the BOP-24/PMS AO7 degradation system showed that •O2-, h+, •OH, and SO4•- are reactive species.
34600883	7	44	theme	reactive	1312:1319	arg1	•O2-					1283:1286	•O2-	1283:1286	•O2-	1283:1286	The free radical capture experiments of the BOP-24/PMS AO7 degradation system showed that •O2-, h+, •OH, and SO4•- are reactive species.
34600883	4	45	theme	PBCD-B-D	836:843	arg1	system					868:873	the 24% PBCD-B-D (BOP-24)/visible light system	828:873	the 24% PBCD-B-D (BOP-24)/visible light system	828:873	Compared to the 24% PBCD-B-D (BOP-24)/visible light system, the degradation efficiency of BOP-24/PMS system for AO7 is increased from 64.1% to 99.2% within shorter time (∼60 min).
34600883	4	46	theme	%	954:954	arg1	%					963:963	64.1% to 99.2%	950:963	64.1% to 99.2%	950:963	Compared to the 24% PBCD-B-D (BOP-24)/visible light system, the degradation efficiency of BOP-24/PMS system for AO7 is increased from 64.1% to 99.2% within shorter time (∼60 min).
34600883	9	47	theme	electron	1506:1513	arg1	PMS					1485:1487	PMS	1485:1487	PMS	1485:1487	PMS also acted as an electron (e-) acceptor to promote the transfer of part of e- to PMS, thereby further improving the efficiency of carrier separation.
34600883	9	47	theme	electron	1506:1513	arg1	acceptor					1520:1527	an electron (e-) acceptor	1503:1527	an electron (e-) acceptor	1503:1527	PMS also acted as an electron (e-) acceptor to promote the transfer of part of e- to PMS, thereby further improving the efficiency of carrier separation.
34600883	4	48	dep	%	963:963	arg1	to					956:957	to	956:957	to	956:957	Compared to the 24% PBCD-B-D (BOP-24)/visible light system, the degradation efficiency of BOP-24/PMS system for AO7 is increased from 64.1% to 99.2% within shorter time (∼60 min).
34600883	10	49	theme	AOP	1776:1778	arg1	technology					1780:1789	SO4•--based AOP technology	1764:1789	SO4•--based AOP technology	1764:1789	The proposed system is an effective method to improve the degradation of pollutants and broadens the range of application of SO4•--based AOP technology.
34600883	1	50	theme	adsorption-photocatalysis	197:221	arg1	combination					182:192	The combination	178:192	The combination of adsorption-photocatalysis and advanced oxidation processes (AOP) based on sulfate (SO4•-) for the treatment of organic pollution	178:324	The combination of adsorption-photocatalysis and advanced oxidation processes (AOP) based on sulfate (SO4•-) for the treatment of organic pollution has the advantages of a high degradation rate, affordability, and an absence of secondary pollution.
34600883	9	51	theme	e-	1516:1517	arg1	PMS					1485:1487	PMS	1485:1487	PMS	1485:1487	PMS also acted as an electron (e-) acceptor to promote the transfer of part of e- to PMS, thereby further improving the efficiency of carrier separation.
34600883	9	51	theme	e-	1516:1517	arg1	acceptor					1520:1527	an electron (e-) acceptor	1503:1527	an electron (e-) acceptor	1503:1527	PMS also acted as an electron (e-) acceptor to promote the transfer of part of e- to PMS, thereby further improving the efficiency of carrier separation.
34600883	10	52	theme	SO4•--based	1764:1774	arg1	technology					1780:1789	SO4•--based AOP technology	1764:1789	SO4•--based AOP technology	1764:1789	The proposed system is an effective method to improve the degradation of pollutants and broadens the range of application of SO4•--based AOP technology.
34600883	1	53	theme	advanced	227:234	arg1	processes					246:254	advanced oxidation processes	227:254	advanced oxidation processes	227:254	The combination of adsorption-photocatalysis and advanced oxidation processes (AOP) based on sulfate (SO4•-) for the treatment of organic pollution has the advantages of a high degradation rate, affordability, and an absence of secondary pollution.
34600883	10	54	theme	application	1749:1759	arg1	range					1740:1744	the range	1736:1744	the range of application of SO4•--based AOP technology	1736:1789	The proposed system is an effective method to improve the degradation of pollutants and broadens the range of application of SO4•--based AOP technology.
34600883	0	55	theme	pollutants	157:166	arg1	degradation					138:148	efficient degradation	128:148	efficient degradation of dye pollutants in water	128:175	Insights into the photocatalytic activation of peroxymonosulfate by visible light over BiOBr-cyclodextrin polymer complexes for efficient degradation of dye pollutants in water.
34600883	2	56	with	AOP	622:624	arg1	functions					574:582	dual functions	569:582	dual functions of adsorption and photocatalysis	569:615	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	1	57	theme	processes	246:254	arg1	combination					182:192	The combination	178:192	The combination of adsorption-photocatalysis and advanced oxidation processes (AOP) based on sulfate (SO4•-) for the treatment of organic pollution	178:324	The combination of adsorption-photocatalysis and advanced oxidation processes (AOP) based on sulfate (SO4•-) for the treatment of organic pollution has the advantages of a high degradation rate, affordability, and an absence of secondary pollution.
34600883	1	58	theme	absence	395:401	arg1	advantages					334:343	the advantages	330:343	the advantages of a high degradation rate, affordability, and an absence of secondary pollution	330:424	The combination of adsorption-photocatalysis and advanced oxidation processes (AOP) based on sulfate (SO4•-) for the treatment of organic pollution has the advantages of a high degradation rate, affordability, and an absence of secondary pollution.
34600883	5	59	theme	pH	1051:1052	arg1	pH = 3-11					1067:1075	pH = 3-11	1067:1075	pH = 3-11	1067:1075	Moreover, the BOP-24/PMS system showed a wide range of pH application (pH = 3-11).
34600883	5	59	theme	pH	1051:1052	arg1	application					1054:1064	pH application	1051:1064	pH application (pH = 3-11)	1051:1076	Moreover, the BOP-24/PMS system showed a wide range of pH application (pH = 3-11).
34600883	2	60	theme	dual	569:572	arg1	functions					574:582	dual functions	569:582	dual functions of adsorption and photocatalysis	569:615	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	7	61	theme	degradation	1252:1262	arg1	system					1264:1269	the BOP-24/PMS AO7 degradation system	1233:1269	the BOP-24/PMS AO7 degradation system	1233:1269	The free radical capture experiments of the BOP-24/PMS AO7 degradation system showed that •O2-, h+, •OH, and SO4•- are reactive species.
34600883	10	62	theme	technology	1780:1789	arg1	application					1749:1759	application	1749:1759	application of SO4•--based AOP technology	1749:1789	The proposed system is an effective method to improve the degradation of pollutants and broadens the range of application of SO4•--based AOP technology.
34600883	1	63	theme	secondary	406:414	arg1	pollution					416:424	secondary pollution	406:424	secondary pollution	406:424	The combination of adsorption-photocatalysis and advanced oxidation processes (AOP) based on sulfate (SO4•-) for the treatment of organic pollution has the advantages of a high degradation rate, affordability, and an absence of secondary pollution.
34600883	10	64	theme	proposed	1643:1650	arg1	system					1652:1657	The proposed system	1639:1657	The proposed system	1639:1657	The proposed system is an effective method to improve the degradation of pollutants and broadens the range of application of SO4•--based AOP technology.
34600883	10	64	theme	proposed	1643:1650	arg1	method					1675:1680	an effective method	1662:1680	an effective method to improve the degradation of pollutants	1662:1721	The proposed system is an effective method to improve the degradation of pollutants and broadens the range of application of SO4•--based AOP technology.
34600883	9	65	theme	part	1556:1559	arg1	transfer					1544:1551	the transfer	1540:1551	the transfer of part of e- to PMS	1540:1572	PMS also acted as an electron (e-) acceptor to promote the transfer of part of e- to PMS, thereby further improving the efficiency of carrier separation.
34600883	8	66	theme	BOP-24	1343:1348	arg1	system					1350:1355	The proposed BOP-24 system	1330:1355	The proposed BOP-24 system	1330:1355	The proposed BOP-24 system used adsorption and a unique cavity structure to enrich AO7 near the active site, thereby reducing the path for PMS activation.
34600883	7	67	theme	BOP-24/PMS	1237:1246	arg1	system					1264:1269	the BOP-24/PMS AO7 degradation system	1233:1269	the BOP-24/PMS AO7 degradation system	1233:1269	The free radical capture experiments of the BOP-24/PMS AO7 degradation system showed that •O2-, h+, •OH, and SO4•- are reactive species.
34600883	9	68	theme	e-	1564:1565	arg1	part					1556:1559	part	1556:1559	part of e-	1556:1565	PMS also acted as an electron (e-) acceptor to promote the transfer of part of e- to PMS, thereby further improving the efficiency of carrier separation.
34600883	6	69	theme	Cl-	1095:1097	arg1	addition					1083:1090	The addition	1079:1090	The addition of Cl-, SO42-, and NO3-	1079:1114	The addition of Cl-, SO42-, and NO3- promoted the photodegradation of AO7, whereas the addition of CO32- did not.
34600883	2	70	from	treatment	649:657	arg1	water					685:689	water	685:689	water	685:689	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	8	71	used	used	1357:1360	arg2	system					1350:1355	The proposed BOP-24 system	1330:1355	The proposed BOP-24 system	1330:1355	The proposed BOP-24 system used adsorption and a unique cavity structure to enrich AO7 near the active site, thereby reducing the path for PMS activation.
34600883	0	72	theme	BiOBr-cyclodextrin	87:104	arg1	complexes					114:122	BiOBr-cyclodextrin polymer complexes	87:122	BiOBr-cyclodextrin polymer complexes for efficient degradation of dye pollutants in water	87:175	Insights into the photocatalytic activation of peroxymonosulfate by visible light over BiOBr-cyclodextrin polymer complexes for efficient degradation of dye pollutants in water.
34600883	1	73	theme	degradation	355:365	arg1	rate					367:370	a high degradation rate	348:370	a high degradation rate	348:370	The combination of adsorption-photocatalysis and advanced oxidation processes (AOP) based on sulfate (SO4•-) for the treatment of organic pollution has the advantages of a high degradation rate, affordability, and an absence of secondary pollution.
34600883	1	74	theme	high	350:353	arg1	rate					367:370	a high degradation rate	348:370	a high degradation rate	348:370	The combination of adsorption-photocatalysis and advanced oxidation processes (AOP) based on sulfate (SO4•-) for the treatment of organic pollution has the advantages of a high degradation rate, affordability, and an absence of secondary pollution.
34600883	8	75	theme	unique	1379:1384	arg1	structure					1393:1401	a unique cavity structure	1377:1401	a unique cavity structure	1377:1401	The proposed BOP-24 system used adsorption and a unique cavity structure to enrich AO7 near the active site, thereby reducing the path for PMS activation.
34600883	3	76	theme	optimal	774:780	arg1	effect					808:813	an optimal adsorption-photocatalytic effect	771:813	an optimal adsorption-photocatalytic effect	771:813	The combination of BiOBr/PBCD-B-D (BOP-24) with peroxymonosulfate (PMS) showed an optimal adsorption-photocatalytic effect.
34600883	0	77	theme	efficient	128:136	arg1	degradation					138:148	efficient degradation	128:148	efficient degradation of dye pollutants in water	128:175	Insights into the photocatalytic activation of peroxymonosulfate by visible light over BiOBr-cyclodextrin polymer complexes for efficient degradation of dye pollutants in water.
34600883	1	78	theme	rate	367:370	arg1	advantages					334:343	the advantages	330:343	the advantages of a high degradation rate, affordability, and an absence of secondary pollution	330:424	The combination of adsorption-photocatalysis and advanced oxidation processes (AOP) based on sulfate (SO4•-) for the treatment of organic pollution has the advantages of a high degradation rate, affordability, and an absence of secondary pollution.
34600883	7	79	theme	radical	1202:1208	arg1	experiments					1218:1228	The free radical capture experiments	1193:1228	The free radical capture experiments of the BOP-24/PMS AO7 degradation system	1193:1269	The free radical capture experiments of the BOP-24/PMS AO7 degradation system showed that •O2-, h+, •OH, and SO4•- are reactive species.
34600883	4	80	theme	BOP-24/PMS	906:915	arg1	system					917:922	BOP-24/PMS system	906:922	BOP-24/PMS system	906:922	Compared to the 24% PBCD-B-D (BOP-24)/visible light system, the degradation efficiency of BOP-24/PMS system for AO7 is increased from 64.1% to 99.2% within shorter time (∼60 min).
34600883	2	81	theme	Orange	667:672	arg1	treatment					649:657	the treatment	645:657	the treatment of Acid Orange 7 (AO7) in water	645:689	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	1	82	theme	affordability	373:385	arg1	advantages					334:343	the advantages	330:343	the advantages of a high degradation rate, affordability, and an absence of secondary pollution	330:424	The combination of adsorption-photocatalysis and advanced oxidation processes (AOP) based on sulfate (SO4•-) for the treatment of organic pollution has the advantages of a high degradation rate, affordability, and an absence of secondary pollution.
34600883	0	83	theme	photocatalytic	18:31	arg1	activation					33:42	the photocatalytic activation	14:42	the photocatalytic activation of peroxymonosulfate by visible light over BiOBr-cyclodextrin polymer complexes for efficient degradation of dye pollutants in water	14:175	Insights into the photocatalytic activation of peroxymonosulfate by visible light over BiOBr-cyclodextrin polymer complexes for efficient degradation of dye pollutants in water.
34600883	9	84	theme	carrier	1619:1625	arg1	separation					1627:1636	carrier separation	1619:1636	carrier separation	1619:1636	PMS also acted as an electron (e-) acceptor to promote the transfer of part of e- to PMS, thereby further improving the efficiency of carrier separation.
34600883	10	85	theme	effective	1665:1673	arg1	system					1652:1657	The proposed system	1639:1657	The proposed system	1639:1657	The proposed system is an effective method to improve the degradation of pollutants and broadens the range of application of SO4•--based AOP technology.
34600883	10	85	theme	effective	1665:1673	arg1	method					1675:1680	an effective method	1662:1680	an effective method to improve the degradation of pollutants	1662:1721	The proposed system is an effective method to improve the degradation of pollutants and broadens the range of application of SO4•--based AOP technology.
34600883	2	86	theme	cyclodextrin	484:495	arg1	PBCD-B-D					504:511	PBCD-B-D	504:511	PBCD-B-D	504:511	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	2	86	theme	cyclodextrin	484:495	arg1	resin					497:501	amphiphilic super-crosslinked porous cyclodextrin resin	447:501	amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D)	447:512	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	8	87	theme	active	1426:1431	arg1	site					1433:1436	the active site	1422:1436	the active site	1422:1436	The proposed BOP-24 system used adsorption and a unique cavity structure to enrich AO7 near the active site, thereby reducing the path for PMS activation.
34600883	2	88	theme	bismuth	515:521	arg1	oxybromide					523:532	bismuth oxybromide	515:532	bismuth oxybromide (BiOBr)	515:540	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	2	88	theme	bismuth	515:521	arg1	BiOBr					535:539	BiOBr	535:539	BiOBr	535:539	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	1	89	theme	pollution	316:324	arg1	treatment					295:303	the treatment	291:303	the treatment of organic pollution	291:324	The combination of adsorption-photocatalysis and advanced oxidation processes (AOP) based on sulfate (SO4•-) for the treatment of organic pollution has the advantages of a high degradation rate, affordability, and an absence of secondary pollution.
34600883	2	90	theme	super-crosslinked	459:475	arg1	PBCD-B-D					504:511	PBCD-B-D	504:511	PBCD-B-D	504:511	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	2	90	theme	super-crosslinked	459:475	arg1	resin					497:501	amphiphilic super-crosslinked porous cyclodextrin resin	447:501	amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D)	447:512	This study combined amphiphilic super-crosslinked porous cyclodextrin resin (PBCD-B-D), bismuth oxybromide (BiOBr), a composite material with dual functions of adsorption and photocatalysis, and AOP based on SO4•- for the treatment of Acid Orange 7 (AO7) in water.
34600883	3	91	with	combination	696:706	arg1	PMS					759:761	PMS	759:761	PMS	759:761	The combination of BiOBr/PBCD-B-D (BOP-24) with peroxymonosulfate (PMS) showed an optimal adsorption-photocatalytic effect.
34600883	3	91	with	combination	696:706	arg1	peroxymonosulfate					740:756	peroxymonosulfate	740:756	peroxymonosulfate (PMS)	740:762	The combination of BiOBr/PBCD-B-D (BOP-24) with peroxymonosulfate (PMS) showed an optimal adsorption-photocatalytic effect.
33896890	0	0	theme	base	92:95	arg1	resin					97:101	heat polymerized denture base resin	67:101	heat polymerized denture base resin	67:101	Mechanical properties and surface roughness of chitosan reinforced heat polymerized denture base resin.
33896890	3	1	from	effect	357:362	arg1	Ra					498:499	Ra	498:499	Ra	498:499	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	3	1	from	effect	357:362	arg1	FT					449:450	FT	449:450	FT	449:450	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	3	1	from	effect	357:362	arg1	FS					424:425	FS	424:425	FS	424:425	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	3	1	from	effect	357:362	arg1	strength					461:468	impact strength	454:468	impact strength (IS)	454:473	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	3	1	from	effect	357:362	arg1	roughness					487:495	surface roughness	479:495	surface roughness (Ra) in heat polymerized DBR	479:524	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	3	1	from	effect	357:362	arg1	strength					414:421	the flexural strength	401:421	the flexural strength (FS)	401:426	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	3	1	from	effect	357:362	arg1	toughness					438:446	fracture toughness	429:446	fracture toughness (FT)	429:451	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	8	2	theme	electron	840:847	arg1	microscope					849:858	scanning electron microscope	831:858	scanning electron microscope	831:858	The tested samples were characterized by scanning electron microscope and Fourier transformation infra-red spectroscopy.
33896890	15	3	theme	Ch	1373:1374	arg1	"					1368:1368	15%wt"	1363:1368	15%wt" of Ch	1363:1374	CONCLUSION The addition of Ch to DBR improved the "FS, FT, IS at 5%wt and Ra at 15%wt" of Ch.
33896890	14	4	from	decrease	1241:1248	arg1	Ra					1253:1254	Ra	1253:1254	Ra	1253:1254	(p <0.001) Ch15 displayed decrease in Ra compared to control group.
33896890	0	5	theme	denture	84:90	arg1	resin					97:101	heat polymerized denture base resin	67:101	heat polymerized denture base resin	67:101	Mechanical properties and surface roughness of chitosan reinforced heat polymerized denture base resin.
33896890	12	6	dep	"	1111:1111	arg1	p<0.001					1103:1109	p<0.001	1103:1109	p<0.001	1103:1109	(p<0.001)".
33896890	8	7	theme	scanning	831:838	arg1	microscope					849:858	scanning electron microscope	831:858	scanning electron microscope	831:858	The tested samples were characterized by scanning electron microscope and Fourier transformation infra-red spectroscopy.
33896890	3	8	from	toughness	438:446	arg1	DBR					522:524	heat polymerized DBR	505:524	heat polymerized DBR	505:524	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	2	9	theme	clinical	303:310	arg1	success					312:318	clinical success	303:318	clinical success of denture	303:329	Ch influence on the mechanical properties of DBR is less studied in the literature and is vital for clinical success of denture.
33896890	3	10	from	strength	461:468	arg1	DBR					522:524	heat polymerized DBR	505:524	heat polymerized DBR	505:524	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	10	11	theme	Ch	1027:1028	arg1	addition					1030:1037	Ch addition	1027:1037	Ch addition	1027:1037	RESULTS FS, FT and IS improved with Ch addition when compared to control group.
33896890	15	12	theme	wt	1366:1367	arg1	"					1368:1368	15%wt"	1363:1368	15%wt" of Ch	1363:1374	CONCLUSION The addition of Ch to DBR improved the "FS, FT, IS at 5%wt and Ra at 15%wt" of Ch.
33896890	7	13	theme	surface	768:774	arg1	profilometer					776:787	non-contact laser surface profilometer	750:787	non-contact laser surface profilometer	750:787	Ra was evaluated by non-contact laser surface profilometer.
33896890	14	14	theme	p	1216:1216	arg1	Ch15					1226:1229	(p <0.001) Ch15	1215:1229	(p <0.001) Ch15	1215:1229	(p <0.001) Ch15 displayed decrease in Ra compared to control group.
33896890	8	15	theme	tested	794:799	arg1	samples					801:807	The tested samples	790:807	The tested samples	790:807	The tested samples were characterized by scanning electron microscope and Fourier transformation infra-red spectroscopy.
33896890	9	16	theme	one-way	947:953	arg1	ANOVA					955:959	one-way ANOVA	947:959	one-way ANOVA	947:959	Data were statistical analyzed with one-way ANOVA and Post hoc Bonferroni test.
33896890	15	17	dep	CONCLUSION	1283:1292	arg1	improved					1320:1327	improved	1320:1327	improved the "FS, FT, IS at 5%wt and Ra at 15%wt" of Ch	1320:1374	CONCLUSION The addition of Ch to DBR improved the "FS, FT, IS at 5%wt and Ra at 15%wt" of Ch.
33896890	8	18	theme	transformation	872:885	arg1	spectroscopy					897:908	Fourier transformation infra-red spectroscopy	864:908	Fourier transformation infra-red spectroscopy	864:908	The tested samples were characterized by scanning electron microscope and Fourier transformation infra-red spectroscopy.
33896890	7	19	theme	non-contact	750:760	arg1	profilometer					776:787	non-contact laser surface profilometer	750:787	non-contact laser surface profilometer	750:787	Ra was evaluated by non-contact laser surface profilometer.
33896890	2	20	theme	DBR	248:250	arg1	properties					234:243	the mechanical properties	219:243	the mechanical properties of DBR	219:250	Ch influence on the mechanical properties of DBR is less studied in the literature and is vital for clinical success of denture.
33896890	5	21	theme	three-point	670:680	arg1	test					690:693	three-point bending test	670:693	three-point bending test	670:693	FS and FT were estimated by three-point bending test.
33896890	1	22	theme	denture	156:162	arg1	resin					169:173	denture base resin	156:173	denture base resin (DBR)	156:179	PURPOSE The antifungal property of chitosan (Ch) in denture base resin (DBR) was well established.
33896890	1	22	theme	denture	156:162	arg1	DBR					176:178	DBR	176:178	DBR	176:178	PURPOSE The antifungal property of chitosan (Ch) in denture base resin (DBR) was well established.
33896890	8	23	theme	Fourier	864:870	arg1	spectroscopy					897:908	Fourier transformation infra-red spectroscopy	864:908	Fourier transformation infra-red spectroscopy	864:908	The tested samples were characterized by scanning electron microscope and Fourier transformation infra-red spectroscopy.
33896890	15	24	theme	%	1365:1365	arg1	"					1368:1368	15%wt"	1363:1368	15%wt" of Ch	1363:1374	CONCLUSION The addition of Ch to DBR improved the "FS, FT, IS at 5%wt and Ra at 15%wt" of Ch.
33896890	3	25	theme	impact	454:459	arg1	strength					461:468	impact strength	454:468	impact strength (IS)	454:473	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	2	26	from	influence	206:214	arg1	properties					234:243	the mechanical properties	219:243	the mechanical properties of DBR	219:250	Ch influence on the mechanical properties of DBR is less studied in the literature and is vital for clinical success of denture.
33896890	1	27	theme	base	164:167	arg1	resin					169:173	denture base resin	156:173	denture base resin (DBR)	156:179	PURPOSE The antifungal property of chitosan (Ch) in denture base resin (DBR) was well established.
33896890	1	27	theme	base	164:167	arg1	DBR					176:178	DBR	176:178	DBR	176:178	PURPOSE The antifungal property of chitosan (Ch) in denture base resin (DBR) was well established.
33896890	11	28	dep	higher	1082:1087	arg1	FS					1090:1091	FS	1090:1091	FS	1090:1091	Ch5 showed higher "FS, FT, IS.
33896890	11	28	dep	higher	1082:1087	arg1	IS					1098:1099	IS	1098:1099	IS	1098:1099	Ch5 showed higher "FS, FT, IS.
33896890	11	28	dep	higher	1082:1087	arg1	FT					1094:1095	FT	1094:1095	FT	1094:1095	Ch5 showed higher "FS, FT, IS.
33896890	1	29	from	chitosan	139:146	arg1	resin					169:173	denture base resin	156:173	denture base resin (DBR)	156:179	PURPOSE The antifungal property of chitosan (Ch) in denture base resin (DBR) was well established.
33896890	1	29	from	chitosan	139:146	arg1	DBR					176:178	DBR	176:178	DBR	176:178	PURPOSE The antifungal property of chitosan (Ch) in denture base resin (DBR) was well established.
33896890	4	30	theme	weight	596:601	arg1	percentage					603:612	weight percentage	596:612	weight percentage	596:612	METHODS A total of 160 samples were divided into 4 groups (n =10) by weight percentage - Ch 0, Ch 5, Ch 10, Ch 15.
33896890	3	31	theme	concentrations	377:390	arg1	effect					357:362	the effect	353:362	the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR	353:524	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	0	32	theme	Mechanical	0:9	arg1	properties					11:20	Mechanical properties	0:20	Mechanical properties	0:20	Mechanical properties and surface roughness of chitosan reinforced heat polymerized denture base resin.
33896890	8	33	theme	infra-red	887:895	arg1	spectroscopy					897:908	Fourier transformation infra-red spectroscopy	864:908	Fourier transformation infra-red spectroscopy	864:908	The tested samples were characterized by scanning electron microscope and Fourier transformation infra-red spectroscopy.
33896890	3	34	theme	surface	479:485	arg1	Ra					498:499	Ra	498:499	Ra	498:499	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	3	34	theme	surface	479:485	arg1	roughness					487:495	surface roughness	479:495	surface roughness (Ra) in heat polymerized DBR	479:524	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	3	35	from	roughness	487:495	arg1	DBR					522:524	heat polymerized DBR	505:524	heat polymerized DBR	505:524	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	4	36	theme	samples	550:556	arg1	total					537:541	A total	535:541	METHODS A total of 160 samples	527:556	METHODS A total of 160 samples were divided into 4 groups (n =10) by weight percentage - Ch 0, Ch 5, Ch 10, Ch 15.
33896890	0	37	theme	surface	26:32	arg1	roughness					34:42	surface roughness	26:42	surface roughness	26:42	Mechanical properties and surface roughness of chitosan reinforced heat polymerized denture base resin.
33896890	15	38	theme	15	1363:1364	arg1	%					1365:1365	%	1365:1365	%	1365:1365	CONCLUSION The addition of Ch to DBR improved the "FS, FT, IS at 5%wt and Ra at 15%wt" of Ch.
33896890	1	39	from	property	127:134	arg1	resin					169:173	denture base resin	156:173	denture base resin (DBR)	156:179	PURPOSE The antifungal property of chitosan (Ch) in denture base resin (DBR) was well established.
33896890	1	39	from	property	127:134	arg1	DBR					176:178	DBR	176:178	DBR	176:178	PURPOSE The antifungal property of chitosan (Ch) in denture base resin (DBR) was well established.
33896890	7	40	theme	laser	762:766	arg1	profilometer					776:787	non-contact laser surface profilometer	750:787	non-contact laser surface profilometer	750:787	Ra was evaluated by non-contact laser surface profilometer.
33896890	15	41	theme	5	1348:1348	arg1	%					1349:1349	%	1349:1349	%	1349:1349	CONCLUSION The addition of Ch to DBR improved the "FS, FT, IS at 5%wt and Ra at 15%wt" of Ch.
33896890	13	42	theme	significant	1161:1171	arg1	differences					1185:1195	significant statistical differences	1161:1195	significant statistical differences among the groups	1161:1212	Increased Ra was observed in Ch5 and Ch10 with significant statistical differences among the groups.
33896890	9	43	theme	hoc	970:972	arg1	test					985:988	Post hoc Bonferroni test	965:988	Post hoc Bonferroni test	965:988	Data were statistical analyzed with one-way ANOVA and Post hoc Bonferroni test.
33896890	6	44	theme	Charpy	717:722	arg1	test					724:727	Charpy test	717:727	Charpy test	717:727	IS was determined by Charpy test.
33896890	1	45	dep	PURPOSE	104:110	arg1	property					127:134	The antifungal property	112:134	PURPOSE The antifungal property of chitosan (Ch) in denture base resin (DBR)	104:179	PURPOSE The antifungal property of chitosan (Ch) in denture base resin (DBR) was well established.
33896890	13	46	theme	statistical	1173:1183	arg1	differences					1185:1195	significant statistical differences	1161:1195	significant statistical differences among the groups	1161:1212	Increased Ra was observed in Ch5 and Ch10 with significant statistical differences among the groups.
33896890	15	47	theme	Ch	1310:1311	arg1	addition					1298:1305	The addition	1294:1305	The addition of Ch to DBR	1294:1318	CONCLUSION The addition of Ch to DBR improved the "FS, FT, IS at 5%wt and Ra at 15%wt" of Ch.
33896890	5	48	theme	bending	682:688	arg1	test					690:693	three-point bending test	670:693	three-point bending test	670:693	FS and FT were estimated by three-point bending test.
33896890	3	49	theme	Ch	395:396	arg1	concentrations					377:390	different concentrations	367:390	different concentrations of Ch	367:396	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	3	50	theme	different	367:375	arg1	concentrations					377:390	different concentrations	367:390	different concentrations of Ch	367:396	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	2	51	theme	Ch	203:204	arg1	influence					206:214	Ch influence	203:214	Ch influence on the mechanical properties of DBR	203:250	Ch influence on the mechanical properties of DBR is less studied in the literature and is vital for clinical success of denture.
33896890	3	52	theme	flexural	405:412	arg1	FS					424:425	FS	424:425	FS	424:425	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	3	52	theme	flexural	405:412	arg1	strength					414:421	the flexural strength	401:421	the flexural strength (FS)	401:426	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	15	53	dep	FS	1334:1335	arg1	wt					1350:1351	5%wt	1348:1351	5%wt	1348:1351	CONCLUSION The addition of Ch to DBR improved the "FS, FT, IS at 5%wt and Ra at 15%wt" of Ch.
33896890	15	53	dep	FS	1334:1335	arg1	Ra					1357:1358	Ra	1357:1358	Ra	1357:1358	CONCLUSION The addition of Ch to DBR improved the "FS, FT, IS at 5%wt and Ra at 15%wt" of Ch.
33896890	4	54	dep	METHODS	527:533	arg1	total					537:541	A total	535:541	METHODS A total of 160 samples	527:556	METHODS A total of 160 samples were divided into 4 groups (n =10) by weight percentage - Ch 0, Ch 5, Ch 10, Ch 15.
33896890	9	55	theme	Post	965:968	arg1	test					985:988	Post hoc Bonferroni test	965:988	Post hoc Bonferroni test	965:988	Data were statistical analyzed with one-way ANOVA and Post hoc Bonferroni test.
33896890	1	56	theme	antifungal	116:125	arg1	property					127:134	The antifungal property	112:134	PURPOSE The antifungal property of chitosan (Ch) in denture base resin (DBR)	104:179	PURPOSE The antifungal property of chitosan (Ch) in denture base resin (DBR) was well established.
33896890	0	57	theme	chitosan	47:54	arg1	properties					11:20	Mechanical properties	0:20	Mechanical properties	0:20	Mechanical properties and surface roughness of chitosan reinforced heat polymerized denture base resin.
33896890	0	57	theme	chitosan	47:54	arg1	roughness					34:42	surface roughness	26:42	surface roughness	26:42	Mechanical properties and surface roughness of chitosan reinforced heat polymerized denture base resin.
33896890	3	58	from	strength	414:421	arg1	DBR					522:524	heat polymerized DBR	505:524	heat polymerized DBR	505:524	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	3	59	theme	fracture	429:436	arg1	FT					449:450	FT	449:450	FT	449:450	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	3	59	theme	fracture	429:436	arg1	toughness					438:446	fracture toughness	429:446	fracture toughness (FT)	429:451	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	10	60	theme	control	1056:1062	arg1	group					1064:1068	control group	1056:1068	control group	1056:1068	RESULTS FS, FT and IS improved with Ch addition when compared to control group.
33896890	14	61	theme	control	1268:1274	arg1	group					1276:1280	control group	1268:1280	control group	1268:1280	(p <0.001) Ch15 displayed decrease in Ra compared to control group.
33896890	15	62	theme	%	1349:1349	arg1	wt					1350:1351	5%wt	1348:1351	5%wt	1348:1351	CONCLUSION The addition of Ch to DBR improved the "FS, FT, IS at 5%wt and Ra at 15%wt" of Ch.
33896890	3	63	theme	polymerized	510:520	arg1	DBR					522:524	heat polymerized DBR	505:524	heat polymerized DBR	505:524	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	9	64	theme	Bonferroni	974:983	arg1	test					985:988	Post hoc Bonferroni test	965:988	Post hoc Bonferroni test	965:988	Data were statistical analyzed with one-way ANOVA and Post hoc Bonferroni test.
33896890	3	65	dep	strength	461:468	arg1	IS					471:472	IS	471:472	IS	471:472	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	2	66	theme	mechanical	223:232	arg1	properties					234:243	the mechanical properties	219:243	the mechanical properties of DBR	219:250	Ch influence on the mechanical properties of DBR is less studied in the literature and is vital for clinical success of denture.
33896890	1	67	from	resin	169:173	arg1	property					127:134	The antifungal property	112:134	PURPOSE The antifungal property of chitosan (Ch) in denture base resin (DBR)	104:179	PURPOSE The antifungal property of chitosan (Ch) in denture base resin (DBR) was well established.
33896890	1	68	theme	chitosan	139:146	arg1	property					127:134	The antifungal property	112:134	PURPOSE The antifungal property of chitosan (Ch) in denture base resin (DBR)	104:179	PURPOSE The antifungal property of chitosan (Ch) in denture base resin (DBR) was well established.
33896890	13	69	theme	Increased	1114:1122	arg1	Ra					1124:1125	Increased Ra	1114:1125	Increased Ra	1114:1125	Increased Ra was observed in Ch5 and Ch10 with significant statistical differences among the groups.
33896890	0	70	theme	polymerized	72:82	arg1	resin					97:101	heat polymerized denture base resin	67:101	heat polymerized denture base resin	67:101	Mechanical properties and surface roughness of chitosan reinforced heat polymerized denture base resin.
33896890	13	71	located	observed	1131:1138	arg1	Ch5					1143:1145	Ch5	1143:1145	Ch5	1143:1145	Increased Ra was observed in Ch5 and Ch10 with significant statistical differences among the groups.
33896890	13	71	located	observed	1131:1138	arg2	Ra					1124:1125	Increased Ra	1114:1125	Increased Ra	1114:1125	Increased Ra was observed in Ch5 and Ch10 with significant statistical differences among the groups.
33896890	13	71	located	observed	1131:1138	arg1	Ch10					1151:1154	Ch10	1151:1154	Ch10	1151:1154	Increased Ra was observed in Ch5 and Ch10 with significant statistical differences among the groups.
33896890	2	72	theme	denture	323:329	arg1	success					312:318	clinical success	303:318	clinical success of denture	303:329	Ch influence on the mechanical properties of DBR is less studied in the literature and is vital for clinical success of denture.
33896890	0	73	theme	heat	67:70	arg1	resin					97:101	heat polymerized denture base resin	67:101	heat polymerized denture base resin	67:101	Mechanical properties and surface roughness of chitosan reinforced heat polymerized denture base resin.
33896890	3	74	theme	heat	505:508	arg1	DBR					522:524	heat polymerized DBR	505:524	heat polymerized DBR	505:524	This study estimates the effect of different concentrations of Ch on the flexural strength (FS), fracture toughness (FT), impact strength (IS) and surface roughness (Ra) in heat polymerized DBR.
33896890	15	75	from	"	1368:1368	arg1	wt					1350:1351	5%wt	1348:1351	5%wt	1348:1351	CONCLUSION The addition of Ch to DBR improved the "FS, FT, IS at 5%wt and Ra at 15%wt" of Ch.
33896890	15	75	from	"	1368:1368	arg1	Ra					1357:1358	Ra	1357:1358	Ra	1357:1358	CONCLUSION The addition of Ch to DBR improved the "FS, FT, IS at 5%wt and Ra at 15%wt" of Ch.
33765544	7	0	theme	less	1188:1191	arg1	stroma					1199:1204	the thicker less stiff stroma	1176:1204	the thicker less stiff stroma	1176:1204	A relatively simple approach is developed that initially considers the cornea as a poro-elastic bi-layer contact problem, that is the presence of an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma.
33765544	3	1	theme	indentation	498:508	arg1	response					510:517	the indentation response	494:517	the indentation response	494:517	Indentation provides a means to quantify the spatial variation of the mechanical properties of the cornea, however the role of the different layers on the indentation response has barely been addressed.
33765544	4	2	theme	fluid	579:583	arg1	content					585:591	the fluid content	575:591	the fluid content	575:591	In addition, the response of the fluid content and its displacement during indentation has not been adequately considered.
33765544	6	3	theme	elastic	865:871	arg1	response					873:880	a classic Hertz elastic response	849:880	a classic Hertz elastic response	849:880	It was observed that the initial phase of loading did not fit a classic Hertz elastic response but showed an initial steeper slope that gradually declines with increasing force and displacement.
33765544	5	4	theme	large	727:731	arg1	indenter					750:757	a relatively large spherical tipped indenter	714:757	a relatively large spherical tipped indenter (R = 500 μm)	714:770	In this study indentation of the cornea with a relatively large spherical tipped indenter (R = 500 μm) is considered.
33765544	5	4	theme	large	727:731	arg1	R = 500 μm					760:769	R = 500 μm	760:769	R = 500 μm	760:769	In this study indentation of the cornea with a relatively large spherical tipped indenter (R = 500 μm) is considered.
33765544	6	5	theme	Hertz	859:863	arg1	response					873:880	a classic Hertz elastic response	849:880	a classic Hertz elastic response	849:880	It was observed that the initial phase of loading did not fit a classic Hertz elastic response but showed an initial steeper slope that gradually declines with increasing force and displacement.
33765544	7	6	theme	bi-layer	1078:1085	arg1	cornea					1053:1058	the cornea	1049:1058	the cornea	1049:1058	A relatively simple approach is developed that initially considers the cornea as a poro-elastic bi-layer contact problem, that is the presence of an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma.
33765544	7	6	theme	bi-layer	1078:1085	arg1	presence					1116:1123	the presence	1112:1123	the presence of an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma	1112:1204	A relatively simple approach is developed that initially considers the cornea as a poro-elastic bi-layer contact problem, that is the presence of an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma.
33765544	7	6	theme	bi-layer	1078:1085	arg1	problem					1095:1101	a poro-elastic bi-layer contact problem	1063:1101	a poro-elastic bi-layer contact problem	1063:1101	A relatively simple approach is developed that initially considers the cornea as a poro-elastic bi-layer contact problem, that is the presence of an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma.
33765544	1	7	theme	Histological	55:66	arg1	observations					68:79	Histological observations	55:79	Histological observations of the cornea	55:93	Histological observations of the cornea have identified the presence of multiple layers with differing thickness and function.
33765544	3	8	theme	cornea	442:447	arg1	properties					424:433	the mechanical properties	409:433	the mechanical properties of the cornea	409:447	Indentation provides a means to quantify the spatial variation of the mechanical properties of the cornea, however the role of the different layers on the indentation response has barely been addressed.
33765544	5	9	theme	spherical	733:741	arg1	indenter					750:757	a relatively large spherical tipped indenter	714:757	a relatively large spherical tipped indenter (R = 500 μm)	714:770	In this study indentation of the cornea with a relatively large spherical tipped indenter (R = 500 μm) is considered.
33765544	5	9	theme	spherical	733:741	arg1	R = 500 μm					760:769	R = 500 μm	760:769	R = 500 μm	760:769	In this study indentation of the cornea with a relatively large spherical tipped indenter (R = 500 μm) is considered.
33765544	6	10	theme	classic	851:857	arg1	response					873:880	a classic Hertz elastic response	849:880	a classic Hertz elastic response	849:880	It was observed that the initial phase of loading did not fit a classic Hertz elastic response but showed an initial steeper slope that gradually declines with increasing force and displacement.
33765544	7	11	theme	contact	1087:1093	arg1	cornea					1053:1058	the cornea	1049:1058	the cornea	1049:1058	A relatively simple approach is developed that initially considers the cornea as a poro-elastic bi-layer contact problem, that is the presence of an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma.
33765544	7	11	theme	contact	1087:1093	arg1	presence					1116:1123	the presence	1112:1123	the presence of an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma	1112:1204	A relatively simple approach is developed that initially considers the cornea as a poro-elastic bi-layer contact problem, that is the presence of an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma.
33765544	7	11	theme	contact	1087:1093	arg1	problem					1095:1101	a poro-elastic bi-layer contact problem	1063:1101	a poro-elastic bi-layer contact problem	1063:1101	A relatively simple approach is developed that initially considers the cornea as a poro-elastic bi-layer contact problem, that is the presence of an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma.
33765544	5	12	with	indentation	683:693	arg1	indenter					750:757	a relatively large spherical tipped indenter	714:757	a relatively large spherical tipped indenter (R = 500 μm)	714:770	In this study indentation of the cornea with a relatively large spherical tipped indenter (R = 500 μm) is considered.
33765544	5	12	with	indentation	683:693	arg1	R = 500 μm					760:769	R = 500 μm	760:769	R = 500 μm	760:769	In this study indentation of the cornea with a relatively large spherical tipped indenter (R = 500 μm) is considered.
33765544	3	13	theme	spatial	388:394	arg1	variation					396:404	the spatial variation	384:404	the spatial variation of the mechanical properties of the cornea	384:447	Indentation provides a means to quantify the spatial variation of the mechanical properties of the cornea, however the role of the different layers on the indentation response has barely been addressed.
33765544	7	14	theme	stiff	1193:1197	arg1	stroma					1199:1204	the thicker less stiff stroma	1176:1204	the thicker less stiff stroma	1176:1204	A relatively simple approach is developed that initially considers the cornea as a poro-elastic bi-layer contact problem, that is the presence of an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma.
33765544	7	15	theme	stiffer	1142:1148	arg1	layer					1159:1163	an outer thin stiffer Bowman's layer	1128:1163	an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma	1128:1204	A relatively simple approach is developed that initially considers the cornea as a poro-elastic bi-layer contact problem, that is the presence of an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma.
33765544	7	16	theme	poro-elastic	1065:1076	arg1	cornea					1053:1058	the cornea	1049:1058	the cornea	1049:1058	A relatively simple approach is developed that initially considers the cornea as a poro-elastic bi-layer contact problem, that is the presence of an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma.
33765544	7	16	theme	poro-elastic	1065:1076	arg1	presence					1116:1123	the presence	1112:1123	the presence of an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma	1112:1204	A relatively simple approach is developed that initially considers the cornea as a poro-elastic bi-layer contact problem, that is the presence of an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma.
33765544	7	16	theme	poro-elastic	1065:1076	arg1	problem					1095:1101	a poro-elastic bi-layer contact problem	1063:1101	a poro-elastic bi-layer contact problem	1063:1101	A relatively simple approach is developed that initially considers the cornea as a poro-elastic bi-layer contact problem, that is the presence of an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma.
33765544	2	17	theme	interstitial	304:315	arg1	component					317:325	an aqueous interstitial component	293:325	an aqueous interstitial component	293:325	The composition of the cornea consists primarily of collagen fibrils held together with proteoglycans but with an aqueous interstitial component being dominant.
33765544	0	18	theme	cornea	19:24	arg1	Indentation					0:10	Indentation	0:10	Indentation of the cornea: A Bi-layer contact problem.	0:53	Indentation of the cornea: A Bi-layer contact problem.
33765544	2	19	theme	aqueous	296:302	arg1	component					317:325	an aqueous interstitial component	293:325	an aqueous interstitial component	293:325	The composition of the cornea consists primarily of collagen fibrils held together with proteoglycans but with an aqueous interstitial component being dominant.
33765544	7	20	theme	thin	1137:1140	arg1	layer					1159:1163	an outer thin stiffer Bowman's layer	1128:1163	an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma	1128:1204	A relatively simple approach is developed that initially considers the cornea as a poro-elastic bi-layer contact problem, that is the presence of an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma.
33765544	3	21	theme	mechanical	413:422	arg1	properties					424:433	the mechanical properties	409:433	the mechanical properties of the cornea	409:447	Indentation provides a means to quantify the spatial variation of the mechanical properties of the cornea, however the role of the different layers on the indentation response has barely been addressed.
33765544	5	22	theme	cornea	702:707	arg1	indentation					683:693	indentation	683:693	indentation of the cornea with a relatively large spherical tipped indenter (R = 500 μm)	683:770	In this study indentation of the cornea with a relatively large spherical tipped indenter (R = 500 μm) is considered.
33765544	3	23	theme	properties	424:433	arg1	variation					396:404	the spatial variation	384:404	the spatial variation of the mechanical properties of the cornea	384:447	Indentation provides a means to quantify the spatial variation of the mechanical properties of the cornea, however the role of the different layers on the indentation response has barely been addressed.
33765544	7	24	theme	outer	1131:1135	arg1	layer					1159:1163	an outer thin stiffer Bowman's layer	1128:1163	an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma	1128:1204	A relatively simple approach is developed that initially considers the cornea as a poro-elastic bi-layer contact problem, that is the presence of an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma.
33765544	7	25	theme	simple	995:1000	arg1	approach					1002:1009	A relatively simple approach	982:1009	A relatively simple approach	982:1009	A relatively simple approach is developed that initially considers the cornea as a poro-elastic bi-layer contact problem, that is the presence of an outer thin stiffer Bowman's layer overlaying the thicker less stiff stroma.
33765544	6	26	theme	initial	896:902	arg1	slope					912:916	an initial steeper slope	893:916	an initial steeper slope that gradually declines with increasing force and displacement	893:979	It was observed that the initial phase of loading did not fit a classic Hertz elastic response but showed an initial steeper slope that gradually declines with increasing force and displacement.
33765544	0	27	theme	contact	38:44	arg1	problem					46:52	A Bi-layer contact problem	27:52	Indentation of the cornea: A Bi-layer contact problem.	0:53	Indentation of the cornea: A Bi-layer contact problem.
33765544	6	28	theme	loading	829:835	arg1	phase					820:824	the initial phase	808:824	the initial phase of loading	808:835	It was observed that the initial phase of loading did not fit a classic Hertz elastic response but showed an initial steeper slope that gradually declines with increasing force and displacement.
33765544	3	29	theme	layers	484:489	arg1	role					462:465	the role	458:465	the role of the different layers on the indentation response	458:517	Indentation provides a means to quantify the spatial variation of the mechanical properties of the cornea, however the role of the different layers on the indentation response has barely been addressed.
33765544	0	30	theme	Bi-layer	29:36	arg1	problem					46:52	A Bi-layer contact problem	27:52	Indentation of the cornea: A Bi-layer contact problem.	0:53	Indentation of the cornea: A Bi-layer contact problem.
33765544	6	31	theme	steeper	904:910	arg1	slope					912:916	an initial steeper slope	893:916	an initial steeper slope that gradually declines with increasing force and displacement	893:979	It was observed that the initial phase of loading did not fit a classic Hertz elastic response but showed an initial steeper slope that gradually declines with increasing force and displacement.
33765544	2	32	theme	cornea	205:210	arg1	composition					186:196	The composition	182:196	The composition of the cornea	182:210	The composition of the cornea consists primarily of collagen fibrils held together with proteoglycans but with an aqueous interstitial component being dominant.
33765544	3	33	from	role	462:465	arg1	response					510:517	the indentation response	494:517	the indentation response	494:517	Indentation provides a means to quantify the spatial variation of the mechanical properties of the cornea, however the role of the different layers on the indentation response has barely been addressed.
33765544	2	34	theme	collagen	234:241	arg1	fibrils					243:249	collagen fibrils	234:249	collagen fibrils held together with proteoglycans but with an aqueous interstitial component	234:325	The composition of the cornea consists primarily of collagen fibrils held together with proteoglycans but with an aqueous interstitial component being dominant.
33765544	1	35	theme	cornea	88:93	arg1	observations					68:79	Histological observations	55:79	Histological observations of the cornea	55:93	Histological observations of the cornea have identified the presence of multiple layers with differing thickness and function.
33765544	6	36	theme	initial	812:818	arg1	phase					820:824	the initial phase	808:824	the initial phase of loading	808:835	It was observed that the initial phase of loading did not fit a classic Hertz elastic response but showed an initial steeper slope that gradually declines with increasing force and displacement.
33765544	3	37	theme	different	474:482	arg1	layers					484:489	the different layers	470:489	the different layers	470:489	Indentation provides a means to quantify the spatial variation of the mechanical properties of the cornea, however the role of the different layers on the indentation response has barely been addressed.
33765544	4	38	theme	content	585:591	arg1	response					563:570	the response	559:570	the response of the fluid content and its displacement during indentation	559:631	In addition, the response of the fluid content and its displacement during indentation has not been adequately considered.
33765544	5	39	theme	tipped	743:748	arg1	indenter					750:757	a relatively large spherical tipped indenter	714:757	a relatively large spherical tipped indenter (R = 500 μm)	714:770	In this study indentation of the cornea with a relatively large spherical tipped indenter (R = 500 μm) is considered.
33765544	5	39	theme	tipped	743:748	arg1	R = 500 μm					760:769	R = 500 μm	760:769	R = 500 μm	760:769	In this study indentation of the cornea with a relatively large spherical tipped indenter (R = 500 μm) is considered.
33765544	1	40	theme	multiple	127:134	arg1	layers					136:141	multiple layers	127:141	multiple layers	127:141	Histological observations of the cornea have identified the presence of multiple layers with differing thickness and function.
33765544	4	41	theme	displacement	601:612	arg1	response					563:570	the response	559:570	the response of the fluid content and its displacement during indentation	559:631	In addition, the response of the fluid content and its displacement during indentation has not been adequately considered.
33765544	0	42	dep	Indentation	0:10	arg1	problem					46:52	A Bi-layer contact problem	27:52	Indentation of the cornea: A Bi-layer contact problem.	0:53	Indentation of the cornea: A Bi-layer contact problem.
33765544	1	43	theme	layers	136:141	arg1	presence					115:122	the presence	111:122	the presence of multiple layers	111:141	Histological observations of the cornea have identified the presence of multiple layers with differing thickness and function.
34389423	11	0	theme	drug	2043:2046	arg1	formulations					2061:2072	drug nanoparticle formulations	2043:2072	drug nanoparticle formulations	2043:2072	The findings of this study clarified the usefulness and necessity of adding polymers and surfactants to SDs to fabricate drug nanoparticle formulations.
34389423	6	1	theme	domains	1133:1139	arg1	size					1112:1115	the enlarged size	1099:1115	the enlarged size	1099:1115	Based on 13C solid-state NMR spectroscopy, this phenomenon should be due to the enlarged size of the PBC-rich domains in SDs, which depended on the decreasing amounts of admixed HPMC.
34389423	10	2	theme	high	1893:1896	arg1	portion					1898:1904	a high portion	1891:1904	a high portion of admixed SDS	1891:1919	Since the increase in size during water immersion, their sizes were still slightly larger than those obtained with a high portion of admixed SDS.
34389423	6	3	theme	admixed	1193:1199	arg1	HPMC					1201:1204	admixed HPMC	1193:1204	admixed HPMC	1193:1204	Based on 13C solid-state NMR spectroscopy, this phenomenon should be due to the enlarged size of the PBC-rich domains in SDs, which depended on the decreasing amounts of admixed HPMC.
34389423	1	4	theme	HPMC	170:173	arg1	sulfate					191:197	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate	141:197	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios	141:260	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	1	4	theme	HPMC	170:173	arg1	SDS					200:202	SDS	200:202	SDS	200:202	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	7	5	theme	nanoparticles	1384:1396	arg1	recrystallization					1349:1365	the particle agglomeration and recrystallization	1318:1365	recrystallization	1349:1365	Although the pre-dissolved part of HPMC had less impact on the primary particle sizes, it was found to inhibit the particle agglomeration and recrystallization of amorphous PBC nanoparticles.
34389423	7	5	theme	nanoparticles	1384:1396	arg1	agglomeration					1331:1343	the particle agglomeration and recrystallization	1318:1365	agglomeration	1331:1343	Although the pre-dissolved part of HPMC had less impact on the primary particle sizes, it was found to inhibit the particle agglomeration and recrystallization of amorphous PBC nanoparticles.
34389423	9	6	theme	pre-dissolved	1624:1636	arg1	SDS					1638:1640	The pre-dissolved SDS	1620:1640	The pre-dissolved SDS	1620:1640	The pre-dissolved SDS could restrain the agglomeration of amorphous PBC nanoparticles, ultimately forming hundreds of irregular nanometer-order structures.
34389423	7	7	theme	particle	1322:1329	arg1	agglomeration					1331:1343	the particle agglomeration and recrystallization	1318:1365	agglomeration	1331:1343	Although the pre-dissolved part of HPMC had less impact on the primary particle sizes, it was found to inhibit the particle agglomeration and recrystallization of amorphous PBC nanoparticles.
34389423	11	8	theme	nanoparticle	2048:2059	arg1	formulations					2061:2072	drug nanoparticle formulations	2043:2072	drug nanoparticle formulations	2043:2072	The findings of this study clarified the usefulness and necessity of adding polymers and surfactants to SDs to fabricate drug nanoparticle formulations.
34389423	1	9	theme	PBC	357:359	arg1	nanoparticles					361:373	amorphous PBC nanoparticles	347:373	amorphous PBC nanoparticles	347:373	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	1	10	theme	/sodium	175:181	arg1	sulfate					191:197	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate	141:197	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios	141:260	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	1	10	theme	/sodium	175:181	arg1	SDS					200:202	SDS	200:202	SDS	200:202	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	2	11	theme	cryogenic	524:532	arg1	cryo-TEM					568:575	cryo-TEM	568:575	cryo-TEM	568:575	The morphological variation of the PBC nanoparticles prepared using SDs of different compositions was determined using dynamic light scattering and cryogenic transmission electron microscopy (cryo-TEM).
34389423	2	11	theme	cryogenic	524:532	arg1	microscopy					556:565	cryogenic transmission electron microscopy	524:565	cryogenic transmission electron microscopy (cryo-TEM)	524:576	The morphological variation of the PBC nanoparticles prepared using SDs of different compositions was determined using dynamic light scattering and cryogenic transmission electron microscopy (cryo-TEM).
34389423	10	12	theme	SDS	1917:1919	arg1	portion					1898:1904	a high portion	1891:1904	a high portion of admixed SDS	1891:1919	Since the increase in size during water immersion, their sizes were still slightly larger than those obtained with a high portion of admixed SDS.
34389423	0	13	theme	solid	123:127	arg1	dispersion					129:138	ternary solid dispersion	115:138	ternary solid dispersion	115:138	Revealing the mechanism of morphological variation of amorphous drug nanoparticles formed by aqueous dispersion of ternary solid dispersion.
34389423	1	14	theme	sulfate	191:197	arg1	SDs					232:234	SDs	232:234	SDs	232:234	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	1	14	theme	sulfate	191:197	arg1	dispersions					219:229	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions	141:229	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios	141:260	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	6	15	theme	decreasing	1171:1180	arg1	amounts					1182:1188	the decreasing amounts	1167:1188	the decreasing amounts of admixed HPMC	1167:1204	Based on 13C solid-state NMR spectroscopy, this phenomenon should be due to the enlarged size of the PBC-rich domains in SDs, which depended on the decreasing amounts of admixed HPMC.
34389423	6	15	theme	decreasing	1171:1180	arg1	HPMC					1201:1204	admixed HPMC	1193:1204	admixed HPMC	1193:1204	Based on 13C solid-state NMR spectroscopy, this phenomenon should be due to the enlarged size of the PBC-rich domains in SDs, which depended on the decreasing amounts of admixed HPMC.
34389423	7	16	contain	had	1247:1249	arg1	part					1234:1237	the pre-dissolved part	1216:1237	the pre-dissolved part of HPMC	1216:1245	Although the pre-dissolved part of HPMC had less impact on the primary particle sizes, it was found to inhibit the particle agglomeration and recrystallization of amorphous PBC nanoparticles.
34389423	7	16	contain	had	1247:1249	arg2	impact					1256:1261	less impact	1251:1261	less impact	1251:1261	Although the pre-dissolved part of HPMC had less impact on the primary particle sizes, it was found to inhibit the particle agglomeration and recrystallization of amorphous PBC nanoparticles.
34389423	3	17	theme	Statistical	579:589	arg1	analysis					591:598	Statistical analysis	579:598	Statistical analysis of particle size versus roundness of PBC nanoparticles	579:653	Statistical analysis of particle size versus roundness of PBC nanoparticles was carried out based on cryo-TEM images.
34389423	4	18	theme	aqueous	858:864	arg1	solution					866:873	an aqueous solution	855:873	an aqueous solution	855:873	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	9	19	theme	PBC	1688:1690	arg1	nanoparticles					1692:1704	amorphous PBC nanoparticles	1678:1704	amorphous PBC nanoparticles	1678:1704	The pre-dissolved SDS could restrain the agglomeration of amorphous PBC nanoparticles, ultimately forming hundreds of irregular nanometer-order structures.
34389423	5	20	theme	amorphous	994:1002	arg1	nanoparticles					1008:1020	discrete amorphous PBC nanoparticles	985:1020	discrete amorphous PBC nanoparticles	985:1020	The admixed HPMC in SDs was demonstrated to play the major role in determining the primary particle sizes of discrete amorphous PBC nanoparticles.
34389423	1	21	theme	nanoparticles	361:373	arg1	formation					334:342	the formation	330:342	the formation of amorphous PBC nanoparticles	330:373	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	5	22	theme	admixed	880:886	arg1	HPMC					888:891	The admixed HPMC	876:891	The admixed HPMC in SDs	876:898	The admixed HPMC in SDs was demonstrated to play the major role in determining the primary particle sizes of discrete amorphous PBC nanoparticles.
34389423	2	23	theme	electron	547:554	arg1	cryo-TEM					568:575	cryo-TEM	568:575	cryo-TEM	568:575	The morphological variation of the PBC nanoparticles prepared using SDs of different compositions was determined using dynamic light scattering and cryogenic transmission electron microscopy (cryo-TEM).
34389423	2	23	theme	electron	547:554	arg1	microscopy					556:565	cryogenic transmission electron microscopy	524:565	cryogenic transmission electron microscopy (cryo-TEM)	524:576	The morphological variation of the PBC nanoparticles prepared using SDs of different compositions was determined using dynamic light scattering and cryogenic transmission electron microscopy (cryo-TEM).
34389423	10	24	theme	water	1810:1814	arg1	immersion					1816:1824	water immersion	1810:1824	water immersion	1810:1824	Since the increase in size during water immersion, their sizes were still slightly larger than those obtained with a high portion of admixed SDS.
34389423	5	25	theme	nanoparticles	1008:1020	arg1	sizes					976:980	the primary particle sizes	955:980	the primary particle sizes of discrete amorphous PBC nanoparticles	955:1020	The admixed HPMC in SDs was demonstrated to play the major role in determining the primary particle sizes of discrete amorphous PBC nanoparticles.
34389423	3	26	theme	size	612:615	arg1	analysis					591:598	Statistical analysis	579:598	Statistical analysis of particle size versus roundness of PBC nanoparticles	579:653	Statistical analysis of particle size versus roundness of PBC nanoparticles was carried out based on cryo-TEM images.
34389423	1	27	theme	Probucol	141:148	arg1	sulfate					191:197	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate	141:197	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios	141:260	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	1	27	theme	Probucol	141:148	arg1	SDS					200:202	SDS	200:202	SDS	200:202	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	11	28	dep	usefulness	1963:1972	arg1	the					1959:1961	the	1959:1961	the	1959:1961	The findings of this study clarified the usefulness and necessity of adding polymers and surfactants to SDs to fabricate drug nanoparticle formulations.
34389423	2	29	theme	compositions	461:472	arg1	SDs					444:446	SDs	444:446	SDs of different compositions	444:472	The morphological variation of the PBC nanoparticles prepared using SDs of different compositions was determined using dynamic light scattering and cryogenic transmission electron microscopy (cryo-TEM).
34389423	7	30	theme	primary	1270:1276	arg1	sizes					1287:1291	the primary particle sizes	1266:1291	the primary particle sizes	1266:1291	Although the pre-dissolved part of HPMC had less impact on the primary particle sizes, it was found to inhibit the particle agglomeration and recrystallization of amorphous PBC nanoparticles.
34389423	0	31	theme	drug	64:67	arg1	variation					41:49	morphological variation	27:49	morphological variation of amorphous drug	27:67	Revealing the mechanism of morphological variation of amorphous drug nanoparticles formed by aqueous dispersion of ternary solid dispersion.
34389423	8	32	theme	sufficient	1418:1427	arg1	SDS					1429:1431	sufficient SDS	1418:1431	sufficient SDS admixed in SDs	1418:1446	On the other hand, sufficient SDS admixed in SDs could suppress the size enhancement of the PBC-rich domains during water immersion and nanoparticle evolution (agglomeration and crystallization) after aqueous dispersion.
34389423	9	33	theme	nanoparticles	1692:1704	arg1	agglomeration					1661:1673	the agglomeration	1657:1673	the agglomeration of amorphous PBC nanoparticles	1657:1704	The pre-dissolved SDS could restrain the agglomeration of amorphous PBC nanoparticles, ultimately forming hundreds of irregular nanometer-order structures.
34389423	1	34	theme	/hypromellose	155:167	arg1	sulfate					191:197	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate	141:197	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios	141:260	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	1	34	theme	/hypromellose	155:167	arg1	SDS					200:202	SDS	200:202	SDS	200:202	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	2	35	theme	dynamic	495:501	arg1	scattering					509:518	dynamic light scattering	495:518	dynamic light scattering	495:518	The morphological variation of the PBC nanoparticles prepared using SDs of different compositions was determined using dynamic light scattering and cryogenic transmission electron microscopy (cryo-TEM).
34389423	11	36	theme	study	1943:1947	arg1	findings					1926:1933	The findings	1922:1933	The findings of this study	1922:1947	The findings of this study clarified the usefulness and necessity of adding polymers and surfactants to SDs to fabricate drug nanoparticle formulations.
34389423	0	37	theme	aqueous	93:99	arg1	dispersion					101:110	aqueous dispersion	93:110	aqueous dispersion of ternary solid dispersion	93:138	Revealing the mechanism of morphological variation of amorphous drug nanoparticles formed by aqueous dispersion of ternary solid dispersion.
34389423	1	38	theme	HPMC	314:317	arg1	effect					304:309	the effect	300:309	the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles	300:373	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	6	39	theme	PBC-rich	1124:1131	arg1	domains					1133:1139	the PBC-rich domains	1120:1139	the PBC-rich domains in SDs	1120:1146	Based on 13C solid-state NMR spectroscopy, this phenomenon should be due to the enlarged size of the PBC-rich domains in SDs, which depended on the decreasing amounts of admixed HPMC.
34389423	9	40	theme	nanometer-order	1748:1762	arg1	structures					1764:1773	irregular nanometer-order structures	1738:1773	irregular nanometer-order structures	1738:1773	The pre-dissolved SDS could restrain the agglomeration of amorphous PBC nanoparticles, ultimately forming hundreds of irregular nanometer-order structures.
34389423	4	41	theme	PBC	762:764	arg1	nanoparticles					766:778	the PBC nanoparticles	758:778	the PBC nanoparticles	758:778	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	1	42	theme	SDS	323:325	arg1	effect					304:309	the effect	300:309	the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles	300:373	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	0	43	theme	variation	41:49	arg1	mechanism					14:22	the mechanism	10:22	the mechanism of morphological variation of amorphous drug	10:67	Revealing the mechanism of morphological variation of amorphous drug nanoparticles formed by aqueous dispersion of ternary solid dispersion.
34389423	5	44	from	HPMC	888:891	arg1	SDs					896:898	SDs	896:898	SDs	896:898	The admixed HPMC in SDs was demonstrated to play the major role in determining the primary particle sizes of discrete amorphous PBC nanoparticles.
34389423	10	45	from	increase	1786:1793	arg1	size					1798:1801	size	1798:1801	size	1798:1801	Since the increase in size during water immersion, their sizes were still slightly larger than those obtained with a high portion of admixed SDS.
34389423	6	46	theme	13C	1032:1034	arg1	spectroscopy					1052:1063	13C solid-state NMR spectroscopy	1032:1063	13C solid-state NMR spectroscopy	1032:1063	Based on 13C solid-state NMR spectroscopy, this phenomenon should be due to the enlarged size of the PBC-rich domains in SDs, which depended on the decreasing amounts of admixed HPMC.
34389423	1	47	theme	various	240:246	arg1	ratios					255:260	various weight ratios	240:260	various weight ratios	240:260	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	8	48	theme	size	1467:1470	arg1	enhancement					1472:1482	the size enhancement	1463:1482	the size enhancement of the PBC-rich domains	1463:1506	On the other hand, sufficient SDS admixed in SDs could suppress the size enhancement of the PBC-rich domains during water immersion and nanoparticle evolution (agglomeration and crystallization) after aqueous dispersion.
34389423	1	49	theme	ratios	255:260	arg1	SDs					232:234	SDs	232:234	SDs	232:234	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	1	49	theme	ratios	255:260	arg1	dispersions					219:229	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions	141:229	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios	141:260	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	3	50	theme	PBC	637:639	arg1	nanoparticles					641:653	PBC nanoparticles	637:653	PBC nanoparticles	637:653	Statistical analysis of particle size versus roundness of PBC nanoparticles was carried out based on cryo-TEM images.
34389423	4	51	theme	HPMC	799:802	arg1	HPMC					799:802	HPMC	799:802	HPMC	799:802	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	4	51	theme	HPMC	799:802	arg1	nanoparticles					766:778	the PBC nanoparticles	758:778	the PBC nanoparticles	758:778	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	4	51	theme	HPMC	799:802	arg1	amounts					788:794	the amounts	784:794	the amounts of HPMC and SDS	784:810	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	4	51	theme	HPMC	799:802	arg1	morphologies					742:753	the morphologies	738:753	the morphologies of the PBC nanoparticles	738:778	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	4	51	theme	HPMC	799:802	arg1	SDS					808:810	SDS	808:810	SDS	808:810	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	0	52	theme	morphological	27:39	arg1	variation					41:49	morphological variation	27:49	morphological variation of amorphous drug	27:67	Revealing the mechanism of morphological variation of amorphous drug nanoparticles formed by aqueous dispersion of ternary solid dispersion.
34389423	2	53	theme	nanoparticles	415:427	arg1	variation					394:402	The morphological variation	376:402	The morphological variation of the PBC nanoparticles prepared using SDs of different compositions	376:472	The morphological variation of the PBC nanoparticles prepared using SDs of different compositions was determined using dynamic light scattering and cryogenic transmission electron microscopy (cryo-TEM).
34389423	8	54	theme	PBC-rich	1491:1498	arg1	domains					1500:1506	the PBC-rich domains	1487:1506	the PBC-rich domains	1487:1506	On the other hand, sufficient SDS admixed in SDs could suppress the size enhancement of the PBC-rich domains during water immersion and nanoparticle evolution (agglomeration and crystallization) after aqueous dispersion.
34389423	3	55	theme	roundness	624:632	arg1	analysis					591:598	Statistical analysis	579:598	Statistical analysis of particle size versus roundness of PBC nanoparticles	579:653	Statistical analysis of particle size versus roundness of PBC nanoparticles was carried out based on cryo-TEM images.
34389423	4	56	theme	SDS	808:810	arg1	HPMC					799:802	HPMC	799:802	HPMC	799:802	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	4	56	theme	SDS	808:810	arg1	nanoparticles					766:778	the PBC nanoparticles	758:778	the PBC nanoparticles	758:778	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	4	56	theme	SDS	808:810	arg1	amounts					788:794	the amounts	784:794	the amounts of HPMC and SDS	784:810	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	4	56	theme	SDS	808:810	arg1	morphologies					742:753	the morphologies	738:753	the morphologies of the PBC nanoparticles	738:778	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	4	56	theme	SDS	808:810	arg1	SDS					808:810	SDS	808:810	SDS	808:810	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	5	57	theme	discrete	985:992	arg1	nanoparticles					1008:1020	discrete amorphous PBC nanoparticles	985:1020	discrete amorphous PBC nanoparticles	985:1020	The admixed HPMC in SDs was demonstrated to play the major role in determining the primary particle sizes of discrete amorphous PBC nanoparticles.
34389423	7	58	theme	pre-dissolved	1220:1232	arg1	part					1234:1237	the pre-dissolved part	1216:1237	the pre-dissolved part of HPMC	1216:1245	Although the pre-dissolved part of HPMC had less impact on the primary particle sizes, it was found to inhibit the particle agglomeration and recrystallization of amorphous PBC nanoparticles.
34389423	8	59	theme	aqueous	1600:1606	arg1	dispersion					1608:1617	aqueous dispersion	1600:1617	aqueous dispersion	1600:1617	On the other hand, sufficient SDS admixed in SDs could suppress the size enhancement of the PBC-rich domains during water immersion and nanoparticle evolution (agglomeration and crystallization) after aqueous dispersion.
34389423	1	60	theme	solid	213:217	arg1	SDs					232:234	SDs	232:234	SDs	232:234	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	1	60	theme	solid	213:217	arg1	dispersions					219:229	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions	141:229	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios	141:260	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	5	61	theme	primary	959:965	arg1	sizes					976:980	the primary particle sizes	955:980	the primary particle sizes of discrete amorphous PBC nanoparticles	955:1020	The admixed HPMC in SDs was demonstrated to play the major role in determining the primary particle sizes of discrete amorphous PBC nanoparticles.
34389423	8	62	theme	nanoparticle	1535:1546	arg1	evolution					1548:1556	nanoparticle evolution	1535:1556	nanoparticle evolution	1535:1556	On the other hand, sufficient SDS admixed in SDs could suppress the size enhancement of the PBC-rich domains during water immersion and nanoparticle evolution (agglomeration and crystallization) after aqueous dispersion.
34389423	6	63	theme	NMR	1048:1050	arg1	spectroscopy					1052:1063	13C solid-state NMR spectroscopy	1032:1063	13C solid-state NMR spectroscopy	1032:1063	Based on 13C solid-state NMR spectroscopy, this phenomenon should be due to the enlarged size of the PBC-rich domains in SDs, which depended on the decreasing amounts of admixed HPMC.
34389423	7	64	theme	amorphous	1370:1378	arg1	nanoparticles					1384:1396	amorphous PBC nanoparticles	1370:1396	amorphous PBC nanoparticles	1370:1396	Although the pre-dissolved part of HPMC had less impact on the primary particle sizes, it was found to inhibit the particle agglomeration and recrystallization of amorphous PBC nanoparticles.
34389423	6	65	theme	HPMC	1201:1204	arg1	amounts					1182:1188	the decreasing amounts	1167:1188	the decreasing amounts of admixed HPMC	1167:1204	Based on 13C solid-state NMR spectroscopy, this phenomenon should be due to the enlarged size of the PBC-rich domains in SDs, which depended on the decreasing amounts of admixed HPMC.
34389423	6	65	theme	HPMC	1201:1204	arg1	HPMC					1201:1204	admixed HPMC	1193:1204	admixed HPMC	1193:1204	Based on 13C solid-state NMR spectroscopy, this phenomenon should be due to the enlarged size of the PBC-rich domains in SDs, which depended on the decreasing amounts of admixed HPMC.
34389423	7	66	theme	PBC	1380:1382	arg1	nanoparticles					1384:1396	amorphous PBC nanoparticles	1370:1396	amorphous PBC nanoparticles	1370:1396	Although the pre-dissolved part of HPMC had less impact on the primary particle sizes, it was found to inhibit the particle agglomeration and recrystallization of amorphous PBC nanoparticles.
34389423	6	67	theme	enlarged	1103:1110	arg1	size					1112:1115	the enlarged size	1099:1115	the enlarged size	1099:1115	Based on 13C solid-state NMR spectroscopy, this phenomenon should be due to the enlarged size of the PBC-rich domains in SDs, which depended on the decreasing amounts of admixed HPMC.
34389423	0	68	theme	dispersion	129:138	arg1	dispersion					101:110	aqueous dispersion	93:110	aqueous dispersion of ternary solid dispersion	93:138	Revealing the mechanism of morphological variation of amorphous drug nanoparticles formed by aqueous dispersion of ternary solid dispersion.
34389423	1	69	theme	dodecyl	183:189	arg1	sulfate					191:197	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate	141:197	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios	141:260	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	1	69	theme	dodecyl	183:189	arg1	SDS					200:202	SDS	200:202	SDS	200:202	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	10	70	theme	admixed	1909:1915	arg1	SDS					1917:1919	admixed SDS	1909:1919	admixed SDS	1909:1919	Since the increase in size during water immersion, their sizes were still slightly larger than those obtained with a high portion of admixed SDS.
34389423	2	71	theme	light	503:507	arg1	scattering					509:518	dynamic light scattering	495:518	dynamic light scattering	495:518	The morphological variation of the PBC nanoparticles prepared using SDs of different compositions was determined using dynamic light scattering and cryogenic transmission electron microscopy (cryo-TEM).
34389423	1	72	theme	amorphous	347:355	arg1	nanoparticles					361:373	amorphous PBC nanoparticles	347:373	amorphous PBC nanoparticles	347:373	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	9	73	theme	amorphous	1678:1686	arg1	nanoparticles					1692:1704	amorphous PBC nanoparticles	1678:1704	amorphous PBC nanoparticles	1678:1704	The pre-dissolved SDS could restrain the agglomeration of amorphous PBC nanoparticles, ultimately forming hundreds of irregular nanometer-order structures.
34389423	8	74	theme	other	1406:1410	arg1	hand					1412:1415	the other hand	1402:1415	the other hand	1402:1415	On the other hand, sufficient SDS admixed in SDs could suppress the size enhancement of the PBC-rich domains during water immersion and nanoparticle evolution (agglomeration and crystallization) after aqueous dispersion.
34389423	5	75	theme	PBC	1004:1006	arg1	nanoparticles					1008:1020	discrete amorphous PBC nanoparticles	985:1020	discrete amorphous PBC nanoparticles	985:1020	The admixed HPMC in SDs was demonstrated to play the major role in determining the primary particle sizes of discrete amorphous PBC nanoparticles.
34389423	3	76	theme	particle	603:610	arg1	size					612:615	particle size	603:615	particle size	603:615	Statistical analysis of particle size versus roundness of PBC nanoparticles was carried out based on cryo-TEM images.
34389423	4	77	dep	observed	721:728	arg1	pre-dissolved					838:850	pre-dissolved	838:850	pre-dissolved in an aqueous solution	838:873	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	4	77	dep	observed	721:728	arg1	either					813:818	either	813:818	either	813:818	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	2	78	theme	transmission	534:545	arg1	cryo-TEM					568:575	cryo-TEM	568:575	cryo-TEM	568:575	The morphological variation of the PBC nanoparticles prepared using SDs of different compositions was determined using dynamic light scattering and cryogenic transmission electron microscopy (cryo-TEM).
34389423	2	78	theme	transmission	534:545	arg1	microscopy					556:565	cryogenic transmission electron microscopy	524:565	cryogenic transmission electron microscopy (cryo-TEM)	524:576	The morphological variation of the PBC nanoparticles prepared using SDs of different compositions was determined using dynamic light scattering and cryogenic transmission electron microscopy (cryo-TEM).
34389423	4	79	theme	clear	699:703	arg1	correlation					705:715	A clear correlation	697:715	A clear correlation	697:715	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	2	80	theme	different	451:459	arg1	compositions					461:472	different compositions	451:472	different compositions	451:472	The morphological variation of the PBC nanoparticles prepared using SDs of different compositions was determined using dynamic light scattering and cryogenic transmission electron microscopy (cryo-TEM).
34389423	7	81	theme	particle	1278:1285	arg1	sizes					1287:1291	the primary particle sizes	1266:1291	the primary particle sizes	1266:1291	Although the pre-dissolved part of HPMC had less impact on the primary particle sizes, it was found to inhibit the particle agglomeration and recrystallization of amorphous PBC nanoparticles.
34389423	6	82	from	SDs	1144:1146	arg1	size					1112:1115	the enlarged size	1099:1115	the enlarged size	1099:1115	Based on 13C solid-state NMR spectroscopy, this phenomenon should be due to the enlarged size of the PBC-rich domains in SDs, which depended on the decreasing amounts of admixed HPMC.
34389423	4	83	from	SDs	831:833	arg1	admixed					820:826	admixed	820:826	admixed	820:826	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	9	84	theme	irregular	1738:1746	arg1	structures					1764:1773	irregular nanometer-order structures	1738:1773	irregular nanometer-order structures	1738:1773	The pre-dissolved SDS could restrain the agglomeration of amorphous PBC nanoparticles, ultimately forming hundreds of irregular nanometer-order structures.
34389423	0	85	theme	ternary	115:121	arg1	dispersion					129:138	ternary solid dispersion	115:138	ternary solid dispersion	115:138	Revealing the mechanism of morphological variation of amorphous drug nanoparticles formed by aqueous dispersion of ternary solid dispersion.
34389423	4	86	from	admixed	820:826	arg1	SDs					831:833	SDs	831:833	SDs	831:833	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	5	87	theme	major	929:933	arg1	role					935:938	the major role	925:938	the major role	925:938	The admixed HPMC in SDs was demonstrated to play the major role in determining the primary particle sizes of discrete amorphous PBC nanoparticles.
34389423	6	88	theme	solid-state	1036:1046	arg1	spectroscopy					1052:1063	13C solid-state NMR spectroscopy	1032:1063	13C solid-state NMR spectroscopy	1032:1063	Based on 13C solid-state NMR spectroscopy, this phenomenon should be due to the enlarged size of the PBC-rich domains in SDs, which depended on the decreasing amounts of admixed HPMC.
34389423	9	89	theme	structures	1764:1773	arg1	hundreds					1726:1733	hundreds	1726:1733	hundreds of irregular nanometer-order structures	1726:1773	The pre-dissolved SDS could restrain the agglomeration of amorphous PBC nanoparticles, ultimately forming hundreds of irregular nanometer-order structures.
34389423	6	90	from	domains	1133:1139	arg1	SDs					1144:1146	SDs	1144:1146	SDs	1144:1146	Based on 13C solid-state NMR spectroscopy, this phenomenon should be due to the enlarged size of the PBC-rich domains in SDs, which depended on the decreasing amounts of admixed HPMC.
34389423	8	91	dep	immersion	1521:1529	arg1	agglomeration					1559:1571	agglomeration	1559:1571	agglomeration	1559:1571	On the other hand, sufficient SDS admixed in SDs could suppress the size enhancement of the PBC-rich domains during water immersion and nanoparticle evolution (agglomeration and crystallization) after aqueous dispersion.
34389423	8	91	dep	immersion	1521:1529	arg1	crystallization					1577:1591	crystallization	1577:1591	crystallization	1577:1591	On the other hand, sufficient SDS admixed in SDs could suppress the size enhancement of the PBC-rich domains during water immersion and nanoparticle evolution (agglomeration and crystallization) after aqueous dispersion.
34389423	7	92	theme	less	1251:1254	arg1	impact					1256:1261	less impact	1251:1261	less impact	1251:1261	Although the pre-dissolved part of HPMC had less impact on the primary particle sizes, it was found to inhibit the particle agglomeration and recrystallization of amorphous PBC nanoparticles.
34389423	4	93	dep	either	813:818	arg1	admixed					820:826	admixed	820:826	admixed	820:826	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	1	94	theme	weight	248:253	arg1	ratios					255:260	various weight ratios	240:260	various weight ratios	240:260	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	0	95	dep	nanoparticles	69:81	arg1	formed					83:88	formed	83:88	nanoparticles formed by aqueous dispersion of ternary solid dispersion	69:138	Revealing the mechanism of morphological variation of amorphous drug nanoparticles formed by aqueous dispersion of ternary solid dispersion.
34389423	7	96	theme	HPMC	1242:1245	arg1	part					1234:1237	the pre-dissolved part	1216:1237	the pre-dissolved part of HPMC	1216:1245	Although the pre-dissolved part of HPMC had less impact on the primary particle sizes, it was found to inhibit the particle agglomeration and recrystallization of amorphous PBC nanoparticles.
34389423	2	97	theme	morphological	380:392	arg1	variation					394:402	The morphological variation	376:402	The morphological variation of the PBC nanoparticles prepared using SDs of different compositions	376:472	The morphological variation of the PBC nanoparticles prepared using SDs of different compositions was determined using dynamic light scattering and cryogenic transmission electron microscopy (cryo-TEM).
34389423	6	98	from	size	1112:1115	arg1	SDs					1144:1146	SDs	1144:1146	SDs	1144:1146	Based on 13C solid-state NMR spectroscopy, this phenomenon should be due to the enlarged size of the PBC-rich domains in SDs, which depended on the decreasing amounts of admixed HPMC.
34389423	1	99	from	effect	304:309	arg1	formation					334:342	the formation	330:342	the formation of amorphous PBC nanoparticles	330:373	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	5	100	theme	particle	967:974	arg1	sizes					976:980	the primary particle sizes	955:980	the primary particle sizes of discrete amorphous PBC nanoparticles	955:1020	The admixed HPMC in SDs was demonstrated to play the major role in determining the primary particle sizes of discrete amorphous PBC nanoparticles.
34389423	8	101	theme	domains	1500:1506	arg1	enhancement					1472:1482	the size enhancement	1463:1482	the size enhancement of the PBC-rich domains	1463:1506	On the other hand, sufficient SDS admixed in SDs could suppress the size enhancement of the PBC-rich domains during water immersion and nanoparticle evolution (agglomeration and crystallization) after aqueous dispersion.
34389423	0	102	theme	amorphous	54:62	arg1	drug					64:67	amorphous drug	54:67	amorphous drug	54:67	Revealing the mechanism of morphological variation of amorphous drug nanoparticles formed by aqueous dispersion of ternary solid dispersion.
34389423	2	103	theme	PBC	411:413	arg1	nanoparticles					415:427	the PBC nanoparticles	407:427	the PBC nanoparticles prepared using SDs of different compositions	407:472	The morphological variation of the PBC nanoparticles prepared using SDs of different compositions was determined using dynamic light scattering and cryogenic transmission electron microscopy (cryo-TEM).
34389423	8	104	theme	water	1515:1519	arg1	immersion					1521:1529	water immersion	1515:1529	water immersion	1515:1529	On the other hand, sufficient SDS admixed in SDs could suppress the size enhancement of the PBC-rich domains during water immersion and nanoparticle evolution (agglomeration and crystallization) after aqueous dispersion.
34389423	3	105	theme	nanoparticles	641:653	arg1	roundness					624:632	roundness	624:632	roundness of PBC nanoparticles	624:653	Statistical analysis of particle size versus roundness of PBC nanoparticles was carried out based on cryo-TEM images.
34389423	4	106	theme	nanoparticles	766:778	arg1	HPMC					799:802	HPMC	799:802	HPMC	799:802	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	4	106	theme	nanoparticles	766:778	arg1	nanoparticles					766:778	the PBC nanoparticles	758:778	the PBC nanoparticles	758:778	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	4	106	theme	nanoparticles	766:778	arg1	amounts					788:794	the amounts	784:794	the amounts of HPMC and SDS	784:810	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	4	106	theme	nanoparticles	766:778	arg1	morphologies					742:753	the morphologies	738:753	the morphologies of the PBC nanoparticles	738:778	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	4	106	theme	nanoparticles	766:778	arg1	SDS					808:810	SDS	808:810	SDS	808:810	A clear correlation was observed between the morphologies of the PBC nanoparticles and the amounts of HPMC and SDS, either admixed in SDs or pre-dissolved in an aqueous solution.
34389423	1	107	theme	ternary	205:211	arg1	SDs					232:234	SDs	232:234	SDs	232:234	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	1	107	theme	ternary	205:211	arg1	dispersions					219:229	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions	141:229	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios	141:260	Probucol (PBC)/hypromellose (HPMC)/sodium dodecyl sulfate (SDS) ternary solid dispersions (SDs) of various weight ratios were prepared and evaluated to unveil the effect of HPMC and SDS on the formation of amorphous PBC nanoparticles.
34389423	3	108	theme	cryo-TEM	680:687	arg1	images					689:694	cryo-TEM images	680:694	cryo-TEM images	680:694	Statistical analysis of particle size versus roundness of PBC nanoparticles was carried out based on cryo-TEM images.
33691231	8	0	theme	different	1092:1100	arg1	concentrations					1108:1121	different final concentrations	1092:1121	different final concentrations (0.5%, 1%, 2% and 3%)	1092:1143	SA/DCP cement composites were synthesized by dissolving different amounts of SA into water (1.0 mL) to obtain different final concentrations (0.5%, 1%, 2% and 3%).
33691231	16	1	theme	incubation	1898:1907	arg1	days					1890:1893	3 days	1888:1893	3 days of incubation in SBF	1888:1914	DCP and SA/DCP cements showed the highest mechanical strength after 3 days of incubation in SBF and declined with prolonged immersion periods.
33691231	2	2	theme	microstructural	312:326	arg1	properties					356:365	the microstructural, mechanical, and biological properties	308:365	the microstructural, mechanical, and biological properties of DCP cements	308:380	In this study, we aimed to investigate the impact of different amounts of sodium alginate (SA) on the microstructural, mechanical, and biological properties of DCP cements.
33691231	9	3	dep	The	1146:1148	arg1	cements					1159:1165	cements	1159:1165	cements	1159:1165	The prepared cements were characterized with XRD, SEM, FTIR and Thermogravimetric analysis (TGA).
33691231	5	4	theme	electron	629:636	arg1	SEM					650:652	SEM	650:652	SEM	650:652	Scanning electron microscopy (SEM) examination revealed that the particles were in globular shape.
33691231	5	4	theme	electron	629:636	arg1	microscopy					638:647	Scanning electron microscopy	620:647	Scanning electron microscopy (SEM) examination	620:665	Scanning electron microscopy (SEM) examination revealed that the particles were in globular shape.
33691231	2	5	theme	different	263:271	arg1	SA					301:302	SA	301:302	SA	301:302	In this study, we aimed to investigate the impact of different amounts of sodium alginate (SA) on the microstructural, mechanical, and biological properties of DCP cements.
33691231	2	5	theme	different	263:271	arg1	amounts					273:279	different amounts	263:279	different amounts of sodium alginate (SA)	263:303	In this study, we aimed to investigate the impact of different amounts of sodium alginate (SA) on the microstructural, mechanical, and biological properties of DCP cements.
33691231	2	5	theme	different	263:271	arg1	alginate					291:298	sodium alginate	284:298	sodium alginate (SA)	284:303	In this study, we aimed to investigate the impact of different amounts of sodium alginate (SA) on the microstructural, mechanical, and biological properties of DCP cements.
33691231	19	6	theme	cells	2284:2288	arg1	viability					2271:2279	viability	2271:2279	viability	2271:2279	All cements were cytocompatible and viability of cells incubated in extracts of cements was higher than observed in the control group.
33691231	19	6	theme	cells	2284:2288	arg1	cytocompatible					2252:2265	cytocompatible	2252:2265	cytocompatible	2252:2265	All cements were cytocompatible and viability of cells incubated in extracts of cements was higher than observed in the control group.
33691231	1	7	theme	Biocompatible	120:132	arg1	phosphate					144:152	Biocompatible dicalcium phosphate	120:152	Biocompatible dicalcium phosphate (DCP)	120:158	Biocompatible dicalcium phosphate (DCP) cements are widely used as bone repair materials.
33691231	1	7	theme	Biocompatible	120:132	arg1	DCP					155:157	DCP	155:157	DCP	155:157	Biocompatible dicalcium phosphate (DCP) cements are widely used as bone repair materials.
33691231	19	8	dep	All	2235:2237	arg1	cements					2239:2245	cements	2239:2245	cements	2239:2245	All cements were cytocompatible and viability of cells incubated in extracts of cements was higher than observed in the control group.
33691231	20	9	theme	bone	2455:2458	arg1	filler					2460:2465	bone filler	2455:2465	bone filler	2455:2465	Based on the outcomes, SA/DCP bone cements have a promising future to be utilized as bone filler.
33691231	6	10	theme	functional	736:745	arg1	groups					747:752	all functional groups	732:752	all functional groups of β-TCP	732:761	Furthermore, all functional groups of β-TCP were also detected using Fourier-transform infrared spectroscopy (FTIR) spectra.
33691231	6	10	theme	functional	736:745	arg1	β-TCP					757:761	β-TCP	757:761	β-TCP	757:761	Furthermore, all functional groups of β-TCP were also detected using Fourier-transform infrared spectroscopy (FTIR) spectra.
33691231	2	11	theme	alginate	291:298	arg1	SA					301:302	SA	301:302	SA	301:302	In this study, we aimed to investigate the impact of different amounts of sodium alginate (SA) on the microstructural, mechanical, and biological properties of DCP cements.
33691231	2	11	theme	alginate	291:298	arg1	amounts					273:279	different amounts	263:279	different amounts of sodium alginate (SA)	263:303	In this study, we aimed to investigate the impact of different amounts of sodium alginate (SA) on the microstructural, mechanical, and biological properties of DCP cements.
33691231	2	11	theme	alginate	291:298	arg1	alginate					291:298	sodium alginate	284:298	sodium alginate (SA)	284:303	In this study, we aimed to investigate the impact of different amounts of sodium alginate (SA) on the microstructural, mechanical, and biological properties of DCP cements.
33691231	1	12	theme	bone	187:190	arg1	materials					199:207	bone repair materials	187:207	bone repair materials	187:207	Biocompatible dicalcium phosphate (DCP) cements are widely used as bone repair materials.
33691231	20	13	dep	cements	2405:2411	arg1	have					2413:2416	have	2413:2416	cements have a promising future to be utilized as bone filler	2405:2465	Based on the outcomes, SA/DCP bone cements have a promising future to be utilized as bone filler.
33691231	18	14	theme	MTT	2174:2176	arg1	assay					2178:2182	MTT assay	2174:2182	MTT assay	2174:2182	Viability and morphology of cells incubated in extract media of DCP and SA/DCP discs after 24 h incubation was studied with MTT assay and fluorescence microscopy imaging, respectively.
33691231	4	15	theme	standard	595:602	arg1	phase					604:608	a standard phase	593:608	a standard phase of β-TCP	593:617	Lattice parameters of the obtained particles determined from X-ray diffraction (XRD), were in good match with a standard phase of β-TCP.
33691231	19	16	theme	cements	2315:2321	arg1	extracts					2303:2310	extracts	2303:2310	extracts of cements was higher than observed in the control group	2303:2367	All cements were cytocompatible and viability of cells incubated in extracts of cements was higher than observed in the control group.
33691231	1	17	theme	dicalcium	134:142	arg1	phosphate					144:152	Biocompatible dicalcium phosphate	120:152	Biocompatible dicalcium phosphate (DCP)	120:158	Biocompatible dicalcium phosphate (DCP) cements are widely used as bone repair materials.
33691231	1	17	theme	dicalcium	134:142	arg1	DCP					155:157	DCP	155:157	DCP	155:157	Biocompatible dicalcium phosphate (DCP) cements are widely used as bone repair materials.
33691231	18	18	theme	DCP	2114:2116	arg1	discs					2129:2133	DCP and SA/DCP discs	2114:2133	discs	2129:2133	Viability and morphology of cells incubated in extract media of DCP and SA/DCP discs after 24 h incubation was studied with MTT assay and fluorescence microscopy imaging, respectively.
33691231	16	19	theme	immersion	1944:1952	arg1	periods					1954:1960	prolonged immersion periods	1934:1960	prolonged immersion periods	1934:1960	DCP and SA/DCP cements showed the highest mechanical strength after 3 days of incubation in SBF and declined with prolonged immersion periods.
33691231	16	20	dep	DCP	1820:1822	arg1	cements					1835:1841	cements	1835:1841	cements	1835:1841	DCP and SA/DCP cements showed the highest mechanical strength after 3 days of incubation in SBF and declined with prolonged immersion periods.
33691231	14	21	theme	2.0	1693:1695	arg1	%					1696:1696	%	1696:1696	%	1696:1696	The highest cumulative weight loss (%) in SBF was observed for 2.0% SA/DCP (about 26.52%) after 21 days of incubation.
33691231	11	22	theme	DCP	1404:1406	arg1	particles					1408:1416	DCP particles	1404:1416	DCP particles	1404:1416	SEM results confirmed that addition of SA inhibited the growth of DCP particles.
33691231	4	23	dep	good	577:580	arg1	match					582:586	match	582:586	match	582:586	Lattice parameters of the obtained particles determined from X-ray diffraction (XRD), were in good match with a standard phase of β-TCP.
33691231	18	24	theme	SA/DCP	2122:2127	arg1	discs					2129:2133	DCP and SA/DCP discs	2114:2133	discs	2129:2133	Viability and morphology of cells incubated in extract media of DCP and SA/DCP discs after 24 h incubation was studied with MTT assay and fluorescence microscopy imaging, respectively.
33691231	0	25	theme	Phosphate	63:71	arg1	Composites					80:89	Dicalcium Phosphate Cement Composites	53:89	Biomechanical Evaluation of an Injectable Alginate / Dicalcium Phosphate Cement Composites for Bone Tissue Engineering.	0:118	Biomechanical Evaluation of an Injectable Alginate / Dicalcium Phosphate Cement Composites for Bone Tissue Engineering.
33691231	7	26	theme	/β-TCP	930:935	arg1	mixture					944:950	monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture	890:950	monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture blended with 1.0 mL of water	890:979	DCP cement (pure phase) was synthesized using monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture blended with 1.0 mL of water.
33691231	12	27	theme	Setting	1419:1425	arg1	time					1427:1430	Setting time	1419:1430	Setting time	1419:1430	Setting time and injectability behaviour were significantly improved upon increasing the SA amount into DCP cements.
33691231	17	28	theme	Dental	2018:2023	arg1	DPSCs					2042:2046	DPSCs	2042:2046	DPSCs	2042:2046	In vitro cell culture experiments were conducted using Dental pulp stem cells (DPSCs).
33691231	17	28	theme	Dental	2018:2023	arg1	cells					2035:2039	Dental pulp stem cells	2018:2039	Dental pulp stem cells (DPSCs)	2018:2047	In vitro cell culture experiments were conducted using Dental pulp stem cells (DPSCs).
33691231	15	29	theme	Ca2+	1759:1762	arg1	ions					1764:1767	Ca2+ ions	1759:1767	Ca2+ ions	1759:1767	Amount of Ca2+ ions released in SBF increased with the addition of SA.
33691231	16	30	from	SBF	1912:1914	arg1	days					1890:1893	3 days	1888:1893	3 days of incubation in SBF	1888:1914	DCP and SA/DCP cements showed the highest mechanical strength after 3 days of incubation in SBF and declined with prolonged immersion periods.
33691231	7	31	theme	MCPM	925:928	arg1	mixture					944:950	monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture	890:950	monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture blended with 1.0 mL of water	890:979	DCP cement (pure phase) was synthesized using monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture blended with 1.0 mL of water.
33691231	17	32	theme	stem	2030:2033	arg1	DPSCs					2042:2046	DPSCs	2042:2046	DPSCs	2042:2046	In vitro cell culture experiments were conducted using Dental pulp stem cells (DPSCs).
33691231	17	32	theme	stem	2030:2033	arg1	cells					2035:2039	Dental pulp stem cells	2018:2039	Dental pulp stem cells (DPSCs)	2018:2047	In vitro cell culture experiments were conducted using Dental pulp stem cells (DPSCs).
33691231	8	33	dep	concentrations	1108:1121	arg1	%					1127:1127	0.5%	1124:1127	0.5%	1124:1127	SA/DCP cement composites were synthesized by dissolving different amounts of SA into water (1.0 mL) to obtain different final concentrations (0.5%, 1%, 2% and 3%).
33691231	8	33	dep	concentrations	1108:1121	arg1	%					1131:1131	1%	1130:1131	1%	1130:1131	SA/DCP cement composites were synthesized by dissolving different amounts of SA into water (1.0 mL) to obtain different final concentrations (0.5%, 1%, 2% and 3%).
33691231	8	33	dep	concentrations	1108:1121	arg1	%					1135:1135	2%	1134:1135	2%	1134:1135	SA/DCP cement composites were synthesized by dissolving different amounts of SA into water (1.0 mL) to obtain different final concentrations (0.5%, 1%, 2% and 3%).
33691231	8	33	dep	concentrations	1108:1121	arg1	%					1142:1142	3%	1141:1142	3%	1141:1142	SA/DCP cement composites were synthesized by dissolving different amounts of SA into water (1.0 mL) to obtain different final concentrations (0.5%, 1%, 2% and 3%).
33691231	0	34	theme	Bone	95:98	arg1	Engineering					107:117	Bone Tissue Engineering	95:117	Bone Tissue Engineering	95:117	Biomechanical Evaluation of an Injectable Alginate / Dicalcium Phosphate Cement Composites for Bone Tissue Engineering.
33691231	8	35	theme	cement	989:994	arg1	composites					996:1005	SA/DCP cement composites	982:1005	SA/DCP cement composites	982:1005	SA/DCP cement composites were synthesized by dissolving different amounts of SA into water (1.0 mL) to obtain different final concentrations (0.5%, 1%, 2% and 3%).
33691231	7	36	theme	monohydrate	912:922	arg1	mixture					944:950	monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture	890:950	monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture blended with 1.0 mL of water	890:979	DCP cement (pure phase) was synthesized using monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture blended with 1.0 mL of water.
33691231	5	37	theme	globular	703:710	arg1	shape					712:716	globular shape	703:716	globular shape	703:716	Scanning electron microscopy (SEM) examination revealed that the particles were in globular shape.
33691231	7	38	theme	monocalcium	890:900	arg1	mixture					944:950	monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture	890:950	monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture blended with 1.0 mL of water	890:979	DCP cement (pure phase) was synthesized using monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture blended with 1.0 mL of water.
33691231	10	39	theme	Monetite	1322:1329	arg1	phase					1331:1335	Monetite phase	1322:1335	Monetite phase	1322:1335	XRD results showed that pure DCP and SA/DCP cements were in a good match with Monetite phase.
33691231	17	40	theme	culture	1977:1983	arg1	experiments					1985:1995	In vitro cell culture experiments	1963:1995	In vitro cell culture experiments	1963:1995	In vitro cell culture experiments were conducted using Dental pulp stem cells (DPSCs).
33691231	2	41	theme	biological	345:354	arg1	properties					356:365	the microstructural, mechanical, and biological properties	308:365	the microstructural, mechanical, and biological properties of DCP cements	308:380	In this study, we aimed to investigate the impact of different amounts of sodium alginate (SA) on the microstructural, mechanical, and biological properties of DCP cements.
33691231	0	42	theme	Biomechanical	0:12	arg1	Evaluation					14:23	Biomechanical Evaluation	0:23	Biomechanical Evaluation of an Injectable Alginate / Dicalcium Phosphate Cement Composites for Bone Tissue Engineering.	0:118	Biomechanical Evaluation of an Injectable Alginate / Dicalcium Phosphate Cement Composites for Bone Tissue Engineering.
33691231	9	43	theme	Thermogravimetric	1210:1226	arg1	TGA					1238:1240	TGA	1238:1240	TGA	1238:1240	The prepared cements were characterized with XRD, SEM, FTIR and Thermogravimetric analysis (TGA).
33691231	9	43	theme	Thermogravimetric	1210:1226	arg1	analysis					1228:1235	Thermogravimetric analysis	1210:1235	Thermogravimetric analysis (TGA)	1210:1241	The prepared cements were characterized with XRD, SEM, FTIR and Thermogravimetric analysis (TGA).
33691231	12	44	theme	SA	1508:1509	arg1	amount					1511:1516	the SA amount	1504:1516	the SA amount	1504:1516	Setting time and injectability behaviour were significantly improved upon increasing the SA amount into DCP cements.
33691231	8	45	theme	different	1038:1046	arg1	SA					1059:1060	SA	1059:1060	SA into water (1.0 mL)	1059:1080	SA/DCP cement composites were synthesized by dissolving different amounts of SA into water (1.0 mL) to obtain different final concentrations (0.5%, 1%, 2% and 3%).
33691231	8	45	theme	different	1038:1046	arg1	amounts					1048:1054	different amounts	1038:1054	different amounts of SA into water (1.0 mL)	1038:1080	SA/DCP cement composites were synthesized by dissolving different amounts of SA into water (1.0 mL) to obtain different final concentrations (0.5%, 1%, 2% and 3%).
33691231	0	46	theme	Injectable	31:40	arg1	Alginate					42:49	an Injectable Alginate	28:49	an Injectable Alginate	28:49	Biomechanical Evaluation of an Injectable Alginate / Dicalcium Phosphate Cement Composites for Bone Tissue Engineering.
33691231	14	47	theme	cumulative	1642:1651	arg1	loss					1660:1663	The highest cumulative weight loss	1630:1663	The highest cumulative weight loss (%) in SBF	1630:1674	The highest cumulative weight loss (%) in SBF was observed for 2.0% SA/DCP (about 26.52%) after 21 days of incubation.
33691231	14	47	theme	cumulative	1642:1651	arg1	%					1666:1666	%	1666:1666	%	1666:1666	The highest cumulative weight loss (%) in SBF was observed for 2.0% SA/DCP (about 26.52%) after 21 days of incubation.
33691231	6	48	theme	FTIR	829:832	arg1	spectra					835:841	Fourier-transform infrared spectroscopy (FTIR) spectra	788:841	Fourier-transform infrared spectroscopy (FTIR) spectra	788:841	Furthermore, all functional groups of β-TCP were also detected using Fourier-transform infrared spectroscopy (FTIR) spectra.
33691231	16	49	from	incubation	1898:1907	arg1	SBF					1912:1914	SBF	1912:1914	SBF	1912:1914	DCP and SA/DCP cements showed the highest mechanical strength after 3 days of incubation in SBF and declined with prolonged immersion periods.
33691231	3	50	theme	precipitation	461:473	arg1	system					475:480	a microwave-assisted wet precipitation system	436:480	a microwave-assisted wet precipitation system	436:480	Beta-tricalcium phosphate (β-TCP) was prepared using a microwave-assisted wet precipitation system.
33691231	10	51	dep	good	1306:1309	arg1	match					1311:1315	match	1311:1315	match	1311:1315	XRD results showed that pure DCP and SA/DCP cements were in a good match with Monetite phase.
33691231	16	52	from	days	1890:1893	arg1	SBF					1912:1914	SBF	1912:1914	SBF	1912:1914	DCP and SA/DCP cements showed the highest mechanical strength after 3 days of incubation in SBF and declined with prolonged immersion periods.
33691231	6	53	theme	spectroscopy	815:826	arg1	spectra					835:841	Fourier-transform infrared spectroscopy (FTIR) spectra	788:841	Fourier-transform infrared spectroscopy (FTIR) spectra	788:841	Furthermore, all functional groups of β-TCP were also detected using Fourier-transform infrared spectroscopy (FTIR) spectra.
33691231	4	54	theme	particles	518:526	arg1	parameters					491:500	Lattice parameters	483:500	Lattice parameters	483:500	Lattice parameters of the obtained particles determined from X-ray diffraction (XRD), were in good match with a standard phase of β-TCP.
33691231	3	55	theme	microwave-assisted	438:455	arg1	system					475:480	a microwave-assisted wet precipitation system	436:480	a microwave-assisted wet precipitation system	436:480	Beta-tricalcium phosphate (β-TCP) was prepared using a microwave-assisted wet precipitation system.
33691231	6	56	theme	Fourier-transform	788:804	arg1	spectra					835:841	Fourier-transform infrared spectroscopy (FTIR) spectra	788:841	Fourier-transform infrared spectroscopy (FTIR) spectra	788:841	Furthermore, all functional groups of β-TCP were also detected using Fourier-transform infrared spectroscopy (FTIR) spectra.
33691231	14	57	theme	incubation	1737:1746	arg1	days					1729:1732	21 days	1726:1732	21 days of incubation	1726:1746	The highest cumulative weight loss (%) in SBF was observed for 2.0% SA/DCP (about 26.52%) after 21 days of incubation.
33691231	17	58	dep	In	1963:1964	arg1	vitro					1966:1970	vitro	1966:1970	vitro	1966:1970	In vitro cell culture experiments were conducted using Dental pulp stem cells (DPSCs).
33691231	10	59	theme	XRD	1244:1246	arg1	results					1248:1254	XRD results	1244:1254	XRD results	1244:1254	XRD results showed that pure DCP and SA/DCP cements were in a good match with Monetite phase.
33691231	18	60	theme	fluorescence	2188:2199	arg1	microscopy					2201:2210	fluorescence microscopy imaging	2188:2218	fluorescence microscopy imaging	2188:2218	Viability and morphology of cells incubated in extract media of DCP and SA/DCP discs after 24 h incubation was studied with MTT assay and fluorescence microscopy imaging, respectively.
33691231	13	61	dep	In	1536:1537	arg1	vitro					1539:1543	vitro	1539:1543	vitro	1539:1543	In vitro biodegradation was evaluated using Simulated body fluid (SBF) over 21 days at 37 °C.
33691231	12	62	dep	DCP	1523:1525	arg1	cements					1527:1533	cements	1527:1533	cements	1527:1533	Setting time and injectability behaviour were significantly improved upon increasing the SA amount into DCP cements.
33691231	16	63	theme	mechanical	1862:1871	arg1	strength					1873:1880	the highest mechanical strength	1850:1880	the highest mechanical strength	1850:1880	DCP and SA/DCP cements showed the highest mechanical strength after 3 days of incubation in SBF and declined with prolonged immersion periods.
33691231	5	64	theme	Scanning	620:627	arg1	SEM					650:652	SEM	650:652	SEM	650:652	Scanning electron microscopy (SEM) examination revealed that the particles were in globular shape.
33691231	5	64	theme	Scanning	620:627	arg1	microscopy					638:647	Scanning electron microscopy	620:647	Scanning electron microscopy (SEM) examination	620:665	Scanning electron microscopy (SEM) examination revealed that the particles were in globular shape.
33691231	10	65	theme	pure	1268:1271	arg1	DCP					1273:1275	pure DCP	1268:1275	pure DCP	1268:1275	XRD results showed that pure DCP and SA/DCP cements were in a good match with Monetite phase.
33691231	2	66	theme	amounts	273:279	arg1	impact					253:258	the impact	249:258	the impact of different amounts of sodium alginate (SA) on the microstructural, mechanical, and biological properties of DCP cements	249:380	In this study, we aimed to investigate the impact of different amounts of sodium alginate (SA) on the microstructural, mechanical, and biological properties of DCP cements.
33691231	7	67	theme	DCP	844:846	arg1	cement					848:853	DCP cement	844:853	DCP cement (pure phase)	844:866	DCP cement (pure phase) was synthesized using monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture blended with 1.0 mL of water.
33691231	7	67	theme	DCP	844:846	arg1	phase					861:865	pure phase	856:865	pure phase	856:865	DCP cement (pure phase) was synthesized using monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture blended with 1.0 mL of water.
33691231	8	68	theme	final	1102:1106	arg1	concentrations					1108:1121	different final concentrations	1092:1121	different final concentrations (0.5%, 1%, 2% and 3%)	1092:1143	SA/DCP cement composites were synthesized by dissolving different amounts of SA into water (1.0 mL) to obtain different final concentrations (0.5%, 1%, 2% and 3%).
33691231	6	69	theme	β-TCP	757:761	arg1	groups					747:752	all functional groups	732:752	all functional groups of β-TCP	732:761	Furthermore, all functional groups of β-TCP were also detected using Fourier-transform infrared spectroscopy (FTIR) spectra.
33691231	6	69	theme	β-TCP	757:761	arg1	β-TCP					757:761	β-TCP	757:761	β-TCP	757:761	Furthermore, all functional groups of β-TCP were also detected using Fourier-transform infrared spectroscopy (FTIR) spectra.
33691231	18	70	theme	24 h	2141:2144	arg1	incubation					2146:2155	24 h incubation	2141:2155	24 h incubation	2141:2155	Viability and morphology of cells incubated in extract media of DCP and SA/DCP discs after 24 h incubation was studied with MTT assay and fluorescence microscopy imaging, respectively.
33691231	4	71	theme	β-TCP	613:617	arg1	phase					604:608	a standard phase	593:608	a standard phase of β-TCP	593:617	Lattice parameters of the obtained particles determined from X-ray diffraction (XRD), were in good match with a standard phase of β-TCP.
33691231	15	72	theme	ions	1764:1767	arg1	Amount					1749:1754	Amount	1749:1754	Amount of Ca2+ ions released in SBF	1749:1783	Amount of Ca2+ ions released in SBF increased with the addition of SA.
33691231	15	72	theme	ions	1764:1767	arg1	ions					1764:1767	Ca2+ ions	1759:1767	Ca2+ ions	1759:1767	Amount of Ca2+ ions released in SBF increased with the addition of SA.
33691231	11	73	theme	particles	1408:1416	arg1	growth					1394:1399	the growth	1390:1399	the growth of DCP particles	1390:1416	SEM results confirmed that addition of SA inhibited the growth of DCP particles.
33691231	2	74	theme	sodium	284:289	arg1	SA					301:302	SA	301:302	SA	301:302	In this study, we aimed to investigate the impact of different amounts of sodium alginate (SA) on the microstructural, mechanical, and biological properties of DCP cements.
33691231	2	74	theme	sodium	284:289	arg1	alginate					291:298	sodium alginate	284:298	sodium alginate (SA)	284:303	In this study, we aimed to investigate the impact of different amounts of sodium alginate (SA) on the microstructural, mechanical, and biological properties of DCP cements.
33691231	1	75	theme	repair	192:197	arg1	materials					199:207	bone repair materials	187:207	bone repair materials	187:207	Biocompatible dicalcium phosphate (DCP) cements are widely used as bone repair materials.
33691231	16	76	theme	prolonged	1934:1942	arg1	periods					1954:1960	prolonged immersion periods	1934:1960	prolonged immersion periods	1934:1960	DCP and SA/DCP cements showed the highest mechanical strength after 3 days of incubation in SBF and declined with prolonged immersion periods.
33691231	12	77	theme	injectability	1436:1448	arg1	behaviour					1450:1458	injectability behaviour	1436:1458	injectability behaviour	1436:1458	Setting time and injectability behaviour were significantly improved upon increasing the SA amount into DCP cements.
33691231	20	78	theme	SA/DCP	2393:2398	arg1	bone					2400:2403	SA/DCP bone	2393:2403	SA/DCP bone	2393:2403	Based on the outcomes, SA/DCP bone cements have a promising future to be utilized as bone filler.
33691231	5	79	theme	microscopy	638:647	arg1	examination					655:665	Scanning electron microscopy (SEM) examination	620:665	Scanning electron microscopy (SEM) examination	620:665	Scanning electron microscopy (SEM) examination revealed that the particles were in globular shape.
33691231	4	80	with	good	577:580	arg1	phase					604:608	a standard phase	593:608	a standard phase of β-TCP	593:617	Lattice parameters of the obtained particles determined from X-ray diffraction (XRD), were in good match with a standard phase of β-TCP.
33691231	7	81	theme	water	975:979	arg1	1.0 mL					965:970	1.0 mL	965:970	1.0 mL of water	965:979	DCP cement (pure phase) was synthesized using monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture blended with 1.0 mL of water.
33691231	14	82	theme	%	1696:1696	arg1	SA/DCP					1698:1703	2.0% SA/DCP	1693:1703	2.0% SA/DCP (about 26.52%)	1693:1718	The highest cumulative weight loss (%) in SBF was observed for 2.0% SA/DCP (about 26.52%) after 21 days of incubation.
33691231	14	82	theme	%	1696:1696	arg1	%					1717:1717	about 26.52%	1706:1717	about 26.52%	1706:1717	The highest cumulative weight loss (%) in SBF was observed for 2.0% SA/DCP (about 26.52%) after 21 days of incubation.
33691231	18	83	theme	extract	2097:2103	arg1	media					2105:2109	extract media	2097:2109	extract media of DCP and SA/DCP discs after 24 h incubation	2097:2155	Viability and morphology of cells incubated in extract media of DCP and SA/DCP discs after 24 h incubation was studied with MTT assay and fluorescence microscopy imaging, respectively.
33691231	13	84	theme	body	1590:1593	arg1	SBF					1602:1604	SBF	1602:1604	SBF	1602:1604	In vitro biodegradation was evaluated using Simulated body fluid (SBF) over 21 days at 37 °C.
33691231	13	84	theme	body	1590:1593	arg1	fluid					1595:1599	Simulated body fluid	1580:1599	Simulated body fluid (SBF)	1580:1605	In vitro biodegradation was evaluated using Simulated body fluid (SBF) over 21 days at 37 °C.
33691231	2	85	from	impact	253:258	arg1	properties					356:365	the microstructural, mechanical, and biological properties	308:365	the microstructural, mechanical, and biological properties of DCP cements	308:380	In this study, we aimed to investigate the impact of different amounts of sodium alginate (SA) on the microstructural, mechanical, and biological properties of DCP cements.
33691231	18	86	theme	discs	2129:2133	arg1	media					2105:2109	extract media	2097:2109	extract media of DCP and SA/DCP discs after 24 h incubation	2097:2155	Viability and morphology of cells incubated in extract media of DCP and SA/DCP discs after 24 h incubation was studied with MTT assay and fluorescence microscopy imaging, respectively.
33691231	11	87	theme	SEM	1338:1340	arg1	results					1342:1348	SEM results	1338:1348	SEM results	1338:1348	SEM results confirmed that addition of SA inhibited the growth of DCP particles.
33691231	0	88	theme	Cement	73:78	arg1	Composites					80:89	Dicalcium Phosphate Cement Composites	53:89	Biomechanical Evaluation of an Injectable Alginate / Dicalcium Phosphate Cement Composites for Bone Tissue Engineering.	0:118	Biomechanical Evaluation of an Injectable Alginate / Dicalcium Phosphate Cement Composites for Bone Tissue Engineering.
33691231	4	89	theme	Lattice	483:489	arg1	parameters					491:500	Lattice parameters	483:500	Lattice parameters	483:500	Lattice parameters of the obtained particles determined from X-ray diffraction (XRD), were in good match with a standard phase of β-TCP.
33691231	11	90	theme	SA	1377:1378	arg1	addition					1365:1372	addition	1365:1372	addition of SA	1365:1378	SEM results confirmed that addition of SA inhibited the growth of DCP particles.
33691231	8	91	theme	SA/DCP	982:987	arg1	composites					996:1005	SA/DCP cement composites	982:1005	SA/DCP cement composites	982:1005	SA/DCP cement composites were synthesized by dissolving different amounts of SA into water (1.0 mL) to obtain different final concentrations (0.5%, 1%, 2% and 3%).
33691231	7	92	theme	powder	937:942	arg1	mixture					944:950	monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture	890:950	monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture blended with 1.0 mL of water	890:979	DCP cement (pure phase) was synthesized using monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture blended with 1.0 mL of water.
33691231	17	93	theme	pulp	2025:2028	arg1	DPSCs					2042:2046	DPSCs	2042:2046	DPSCs	2042:2046	In vitro cell culture experiments were conducted using Dental pulp stem cells (DPSCs).
33691231	17	93	theme	pulp	2025:2028	arg1	cells					2035:2039	Dental pulp stem cells	2018:2039	Dental pulp stem cells (DPSCs)	2018:2047	In vitro cell culture experiments were conducted using Dental pulp stem cells (DPSCs).
33691231	13	94	theme	Simulated	1580:1588	arg1	SBF					1602:1604	SBF	1602:1604	SBF	1602:1604	In vitro biodegradation was evaluated using Simulated body fluid (SBF) over 21 days at 37 °C.
33691231	13	94	theme	Simulated	1580:1588	arg1	fluid					1595:1599	Simulated body fluid	1580:1599	Simulated body fluid (SBF)	1580:1605	In vitro biodegradation was evaluated using Simulated body fluid (SBF) over 21 days at 37 °C.
33691231	0	95	theme	Tissue	100:105	arg1	Engineering					107:117	Bone Tissue Engineering	95:117	Bone Tissue Engineering	95:117	Biomechanical Evaluation of an Injectable Alginate / Dicalcium Phosphate Cement Composites for Bone Tissue Engineering.
33691231	19	96	theme	control	2355:2361	arg1	group					2363:2367	the control group	2351:2367	the control group	2351:2367	All cements were cytocompatible and viability of cells incubated in extracts of cements was higher than observed in the control group.
33691231	16	97	theme	highest	1854:1860	arg1	strength					1873:1880	the highest mechanical strength	1850:1880	the highest mechanical strength	1850:1880	DCP and SA/DCP cements showed the highest mechanical strength after 3 days of incubation in SBF and declined with prolonged immersion periods.
33691231	17	98	theme	In	1963:1964	arg1	experiments					1985:1995	In vitro cell culture experiments	1963:1995	In vitro cell culture experiments	1963:1995	In vitro cell culture experiments were conducted using Dental pulp stem cells (DPSCs).
33691231	13	99	from	37 °C	1623:1627	arg1	days					1615:1618	21 days	1612:1618	21 days at 37 °C	1612:1627	In vitro biodegradation was evaluated using Simulated body fluid (SBF) over 21 days at 37 °C.
33691231	17	100	theme	cell	1972:1975	arg1	experiments					1985:1995	In vitro cell culture experiments	1963:1995	In vitro cell culture experiments	1963:1995	In vitro cell culture experiments were conducted using Dental pulp stem cells (DPSCs).
33691231	14	101	from	loss	1660:1663	arg1	SBF					1672:1674	SBF	1672:1674	SBF	1672:1674	The highest cumulative weight loss (%) in SBF was observed for 2.0% SA/DCP (about 26.52%) after 21 days of incubation.
33691231	7	102	theme	phosphate	902:910	arg1	mixture					944:950	monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture	890:950	monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture blended with 1.0 mL of water	890:979	DCP cement (pure phase) was synthesized using monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture blended with 1.0 mL of water.
33691231	10	103	with	good	1306:1309	arg1	phase					1331:1335	Monetite phase	1322:1335	Monetite phase	1322:1335	XRD results showed that pure DCP and SA/DCP cements were in a good match with Monetite phase.
33691231	4	104	theme	X-ray	544:548	arg1	XRD					563:565	XRD	563:565	XRD	563:565	Lattice parameters of the obtained particles determined from X-ray diffraction (XRD), were in good match with a standard phase of β-TCP.
33691231	4	104	theme	X-ray	544:548	arg1	diffraction					550:560	X-ray diffraction	544:560	X-ray diffraction (XRD)	544:566	Lattice parameters of the obtained particles determined from X-ray diffraction (XRD), were in good match with a standard phase of β-TCP.
33691231	15	105	theme	SA	1816:1817	arg1	addition					1804:1811	the addition	1800:1811	the addition of SA	1800:1817	Amount of Ca2+ ions released in SBF increased with the addition of SA.
33691231	18	106	theme	imaging	2212:2218	arg1	microscopy					2201:2210	fluorescence microscopy imaging	2188:2218	fluorescence microscopy imaging	2188:2218	Viability and morphology of cells incubated in extract media of DCP and SA/DCP discs after 24 h incubation was studied with MTT assay and fluorescence microscopy imaging, respectively.
33691231	0	107	theme	Alginate	42:49	arg1	Evaluation					14:23	Biomechanical Evaluation	0:23	Biomechanical Evaluation of an Injectable Alginate / Dicalcium Phosphate Cement Composites for Bone Tissue Engineering.	0:118	Biomechanical Evaluation of an Injectable Alginate / Dicalcium Phosphate Cement Composites for Bone Tissue Engineering.
33691231	14	108	theme	weight	1653:1658	arg1	loss					1660:1663	The highest cumulative weight loss	1630:1663	The highest cumulative weight loss (%) in SBF	1630:1674	The highest cumulative weight loss (%) in SBF was observed for 2.0% SA/DCP (about 26.52%) after 21 days of incubation.
33691231	14	108	theme	weight	1653:1658	arg1	%					1666:1666	%	1666:1666	%	1666:1666	The highest cumulative weight loss (%) in SBF was observed for 2.0% SA/DCP (about 26.52%) after 21 days of incubation.
33691231	8	109	theme	SA	1059:1060	arg1	SA					1059:1060	SA	1059:1060	SA into water (1.0 mL)	1059:1080	SA/DCP cement composites were synthesized by dissolving different amounts of SA into water (1.0 mL) to obtain different final concentrations (0.5%, 1%, 2% and 3%).
33691231	8	109	theme	SA	1059:1060	arg1	amounts					1048:1054	different amounts	1038:1054	different amounts of SA into water (1.0 mL)	1038:1080	SA/DCP cement composites were synthesized by dissolving different amounts of SA into water (1.0 mL) to obtain different final concentrations (0.5%, 1%, 2% and 3%).
33691231	18	110	theme	cells	2078:2082	arg1	morphology					2064:2073	morphology	2064:2073	morphology	2064:2073	Viability and morphology of cells incubated in extract media of DCP and SA/DCP discs after 24 h incubation was studied with MTT assay and fluorescence microscopy imaging, respectively.
33691231	18	110	theme	cells	2078:2082	arg1	Viability					2050:2058	Viability	2050:2058	Viability	2050:2058	Viability and morphology of cells incubated in extract media of DCP and SA/DCP discs after 24 h incubation was studied with MTT assay and fluorescence microscopy imaging, respectively.
33691231	3	111	theme	Beta-tricalcium	383:397	arg1	β-TCP					410:414	β-TCP	410:414	β-TCP	410:414	Beta-tricalcium phosphate (β-TCP) was prepared using a microwave-assisted wet precipitation system.
33691231	3	111	theme	Beta-tricalcium	383:397	arg1	phosphate					399:407	Beta-tricalcium phosphate	383:407	Beta-tricalcium phosphate (β-TCP)	383:415	Beta-tricalcium phosphate (β-TCP) was prepared using a microwave-assisted wet precipitation system.
33691231	4	112	theme	obtained	509:516	arg1	particles					518:526	the obtained particles	505:526	the obtained particles	505:526	Lattice parameters of the obtained particles determined from X-ray diffraction (XRD), were in good match with a standard phase of β-TCP.
33691231	13	113	theme	In	1536:1537	arg1	biodegradation					1545:1558	In vitro biodegradation	1536:1558	In vitro biodegradation	1536:1558	In vitro biodegradation was evaluated using Simulated body fluid (SBF) over 21 days at 37 °C.
33691231	2	114	dep	DCP	370:372	arg1	cements					374:380	cements	374:380	cements	374:380	In this study, we aimed to investigate the impact of different amounts of sodium alginate (SA) on the microstructural, mechanical, and biological properties of DCP cements.
33691231	2	115	theme	DCP	370:372	arg1	properties					356:365	the microstructural, mechanical, and biological properties	308:365	the microstructural, mechanical, and biological properties of DCP cements	308:380	In this study, we aimed to investigate the impact of different amounts of sodium alginate (SA) on the microstructural, mechanical, and biological properties of DCP cements.
33691231	0	116	theme	Dicalcium	53:61	arg1	Composites					80:89	Dicalcium Phosphate Cement Composites	53:89	Biomechanical Evaluation of an Injectable Alginate / Dicalcium Phosphate Cement Composites for Bone Tissue Engineering.	0:118	Biomechanical Evaluation of an Injectable Alginate / Dicalcium Phosphate Cement Composites for Bone Tissue Engineering.
33691231	14	117	theme	highest	1634:1640	arg1	loss					1660:1663	The highest cumulative weight loss	1630:1663	The highest cumulative weight loss (%) in SBF	1630:1674	The highest cumulative weight loss (%) in SBF was observed for 2.0% SA/DCP (about 26.52%) after 21 days of incubation.
33691231	14	117	theme	highest	1634:1640	arg1	%					1666:1666	%	1666:1666	%	1666:1666	The highest cumulative weight loss (%) in SBF was observed for 2.0% SA/DCP (about 26.52%) after 21 days of incubation.
33691231	6	118	theme	infrared	806:813	arg1	spectra					835:841	Fourier-transform infrared spectroscopy (FTIR) spectra	788:841	Fourier-transform infrared spectroscopy (FTIR) spectra	788:841	Furthermore, all functional groups of β-TCP were also detected using Fourier-transform infrared spectroscopy (FTIR) spectra.
33691231	3	119	theme	wet	457:459	arg1	system					475:480	a microwave-assisted wet precipitation system	436:480	a microwave-assisted wet precipitation system	436:480	Beta-tricalcium phosphate (β-TCP) was prepared using a microwave-assisted wet precipitation system.
33691231	7	120	theme	pure	856:859	arg1	cement					848:853	DCP cement	844:853	DCP cement (pure phase)	844:866	DCP cement (pure phase) was synthesized using monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture blended with 1.0 mL of water.
33691231	7	120	theme	pure	856:859	arg1	phase					861:865	pure phase	856:865	pure phase	856:865	DCP cement (pure phase) was synthesized using monocalcium phosphate monohydrate (MCPM)/β-TCP powder mixture blended with 1.0 mL of water.
33691231	0	121	dep	Evaluation	14:23	arg1	Composites					80:89	Dicalcium Phosphate Cement Composites	53:89	Biomechanical Evaluation of an Injectable Alginate / Dicalcium Phosphate Cement Composites for Bone Tissue Engineering.	0:118	Biomechanical Evaluation of an Injectable Alginate / Dicalcium Phosphate Cement Composites for Bone Tissue Engineering.
33691231	2	122	theme	mechanical	329:338	arg1	properties					356:365	the microstructural, mechanical, and biological properties	308:365	the microstructural, mechanical, and biological properties of DCP cements	308:380	In this study, we aimed to investigate the impact of different amounts of sodium alginate (SA) on the microstructural, mechanical, and biological properties of DCP cements.
32445426	1	0	theme	controlled	188:197	arg1	self-assembly					208:220	thermodynamically controlled covalent self-assembly	170:220	thermodynamically controlled covalent self-assembly	170:220	Enzyme-mediated dynamic combinatorial chemistry combines the concept of thermodynamically controlled covalent self-assembly with the inherent biological relevance of enzymatic transformations.
32445426	4	1	theme	cyclodextrins	627:639	arg1	libraries					614:622	Dynamic libraries	606:622	Dynamic libraries of cyclodextrins (CDs)	606:645	Dynamic libraries of cyclodextrins (CDs) could be obtained in wide ranges of pH (5.0-9.0), temperature (5-37 °C), and salinity (up to 7.5 m NaNO3 ), and with high organic solvent content (50 % by volume of ethanol), showing that enzyme-mediated dynamic systems can be robust and not limited to physiological conditions.
32445426	0	2	theme	Template	80:87	arg1	Effects					89:95	Template Effects	80:95	Template Effects	80:95	Tuning the Outcome of Enzyme-Mediated Dynamic Cyclodextrin Libraries to Enhance Template Effects.
32445426	1	3	theme	covalent	199:206	arg1	self-assembly					208:220	thermodynamically controlled covalent self-assembly	170:220	thermodynamically controlled covalent self-assembly	170:220	Enzyme-mediated dynamic combinatorial chemistry combines the concept of thermodynamically controlled covalent self-assembly with the inherent biological relevance of enzymatic transformations.
32445426	5	4	theme	α-CD	1088:1091	arg1	production					1074:1083	highly selective production	1057:1083	highly selective production of α-CD, an otherwise challenging target due to competition from the structurally similar β-CD	1057:1178	Furthermore, it is demonstrated how strategic choice of reaction conditions can enhance template effects, in this case, to achieve highly selective production of α-CD, an otherwise challenging target due to competition from the structurally similar β-CD.
32445426	3	5	theme	dynamic	576:582	arg1	library					597:603	the dynamic cyclodextrin library	572:603	the dynamic cyclodextrin library	572:603	External factors, such as pH, temperature, solvent, and salinity are reported to modulate the composition of the dynamic cyclodextrin library.
32445426	3	6	theme	library	597:603	arg1	composition					557:567	the composition	553:567	the composition of the dynamic cyclodextrin library	553:603	External factors, such as pH, temperature, solvent, and salinity are reported to modulate the composition of the dynamic cyclodextrin library.
32445426	3	7	theme	cyclodextrin	584:595	arg1	library					597:603	the dynamic cyclodextrin library	572:603	the dynamic cyclodextrin library	572:603	External factors, such as pH, temperature, solvent, and salinity are reported to modulate the composition of the dynamic cyclodextrin library.
32445426	5	8	theme	similar	1167:1173	arg1	β-CD					1175:1178	the structurally similar β-CD	1150:1178	the structurally similar β-CD	1150:1178	Furthermore, it is demonstrated how strategic choice of reaction conditions can enhance template effects, in this case, to achieve highly selective production of α-CD, an otherwise challenging target due to competition from the structurally similar β-CD.
32445426	1	9	theme	self-assembly	208:220	arg1	concept					159:165	the concept	155:165	the concept of thermodynamically controlled covalent self-assembly	155:220	Enzyme-mediated dynamic combinatorial chemistry combines the concept of thermodynamically controlled covalent self-assembly with the inherent biological relevance of enzymatic transformations.
32445426	3	10	theme	External	463:470	arg1	pH					489:490	pH	489:490	pH	489:490	External factors, such as pH, temperature, solvent, and salinity are reported to modulate the composition of the dynamic cyclodextrin library.
32445426	3	10	theme	External	463:470	arg1	solvent					506:512	solvent	506:512	solvent	506:512	External factors, such as pH, temperature, solvent, and salinity are reported to modulate the composition of the dynamic cyclodextrin library.
32445426	3	10	theme	External	463:470	arg1	factors					472:478	External factors	463:478	External factors	463:478	External factors, such as pH, temperature, solvent, and salinity are reported to modulate the composition of the dynamic cyclodextrin library.
32445426	3	10	theme	External	463:470	arg1	salinity					519:526	salinity	519:526	salinity	519:526	External factors, such as pH, temperature, solvent, and salinity are reported to modulate the composition of the dynamic cyclodextrin library.
32445426	3	10	theme	External	463:470	arg1	temperature					493:503	temperature	493:503	temperature	493:503	External factors, such as pH, temperature, solvent, and salinity are reported to modulate the composition of the dynamic cyclodextrin library.
32445426	4	11	theme	pH	683:684	arg1	ranges					673:678	wide ranges	668:678	wide ranges of pH (5.0-9.0), temperature (5-37 °C), and salinity (up to 7.5 m NaNO3 )	668:752	Dynamic libraries of cyclodextrins (CDs) could be obtained in wide ranges of pH (5.0-9.0), temperature (5-37 °C), and salinity (up to 7.5 m NaNO3 ), and with high organic solvent content (50 % by volume of ethanol), showing that enzyme-mediated dynamic systems can be robust and not limited to physiological conditions.
32445426	4	12	theme	ethanol	812:818	arg1	volume					802:807	volume	802:807	volume of ethanol	802:818	Dynamic libraries of cyclodextrins (CDs) could be obtained in wide ranges of pH (5.0-9.0), temperature (5-37 °C), and salinity (up to 7.5 m NaNO3 ), and with high organic solvent content (50 % by volume of ethanol), showing that enzyme-mediated dynamic systems can be robust and not limited to physiological conditions.
32445426	5	13	theme	challenging	1107:1117	arg1	target					1119:1124	an otherwise challenging target	1094:1124	an otherwise challenging target due to competition from the structurally similar β-CD	1094:1178	Furthermore, it is demonstrated how strategic choice of reaction conditions can enhance template effects, in this case, to achieve highly selective production of α-CD, an otherwise challenging target due to competition from the structurally similar β-CD.
32445426	5	13	theme	challenging	1107:1117	arg1	α-CD					1088:1091	α-CD	1088:1091	α-CD	1088:1091	Furthermore, it is demonstrated how strategic choice of reaction conditions can enhance template effects, in this case, to achieve highly selective production of α-CD, an otherwise challenging target due to competition from the structurally similar β-CD.
32445426	1	14	theme	Enzyme-mediated	98:112	arg1	chemistry					136:144	Enzyme-mediated dynamic combinatorial chemistry	98:144	Enzyme-mediated dynamic combinatorial chemistry	98:144	Enzyme-mediated dynamic combinatorial chemistry combines the concept of thermodynamically controlled covalent self-assembly with the inherent biological relevance of enzymatic transformations.
32445426	2	15	theme	glucanotransferase	434:451	arg1	action					411:416	the action	407:416	the action of cyclodextrin glucanotransferase (CGTase)	407:460	A system of interconverting cyclodextrins has been explored, in which the glycosidic linkage is rendered dynamic by the action of cyclodextrin glucanotransferase (CGTase).
32445426	4	16	theme	solvent	777:783	arg1	content					785:791	high organic solvent content	764:791	high organic solvent content (50 % by volume of ethanol)	764:819	Dynamic libraries of cyclodextrins (CDs) could be obtained in wide ranges of pH (5.0-9.0), temperature (5-37 °C), and salinity (up to 7.5 m NaNO3 ), and with high organic solvent content (50 % by volume of ethanol), showing that enzyme-mediated dynamic systems can be robust and not limited to physiological conditions.
32445426	1	17	theme	dynamic	114:120	arg1	chemistry					136:144	Enzyme-mediated dynamic combinatorial chemistry	98:144	Enzyme-mediated dynamic combinatorial chemistry	98:144	Enzyme-mediated dynamic combinatorial chemistry combines the concept of thermodynamically controlled covalent self-assembly with the inherent biological relevance of enzymatic transformations.
32445426	1	18	theme	biological	240:249	arg1	relevance					251:259	the inherent biological relevance	227:259	the inherent biological relevance of enzymatic transformations	227:288	Enzyme-mediated dynamic combinatorial chemistry combines the concept of thermodynamically controlled covalent self-assembly with the inherent biological relevance of enzymatic transformations.
32445426	2	19	theme	glycosidic	365:374	arg1	linkage					376:382	the glycosidic linkage	361:382	the glycosidic linkage	361:382	A system of interconverting cyclodextrins has been explored, in which the glycosidic linkage is rendered dynamic by the action of cyclodextrin glucanotransferase (CGTase).
32445426	1	20	theme	combinatorial	122:134	arg1	chemistry					136:144	Enzyme-mediated dynamic combinatorial chemistry	98:144	Enzyme-mediated dynamic combinatorial chemistry	98:144	Enzyme-mediated dynamic combinatorial chemistry combines the concept of thermodynamically controlled covalent self-assembly with the inherent biological relevance of enzymatic transformations.
32445426	4	21	theme	temperature	697:707	arg1	ranges					673:678	wide ranges	668:678	wide ranges of pH (5.0-9.0), temperature (5-37 °C), and salinity (up to 7.5 m NaNO3 )	668:752	Dynamic libraries of cyclodextrins (CDs) could be obtained in wide ranges of pH (5.0-9.0), temperature (5-37 °C), and salinity (up to 7.5 m NaNO3 ), and with high organic solvent content (50 % by volume of ethanol), showing that enzyme-mediated dynamic systems can be robust and not limited to physiological conditions.
32445426	0	22	theme	Dynamic	38:44	arg1	Libraries					59:67	Enzyme-Mediated Dynamic Cyclodextrin Libraries	22:67	Enzyme-Mediated Dynamic Cyclodextrin Libraries	22:67	Tuning the Outcome of Enzyme-Mediated Dynamic Cyclodextrin Libraries to Enhance Template Effects.
32445426	4	23	theme	Dynamic	606:612	arg1	libraries					614:622	Dynamic libraries	606:622	Dynamic libraries of cyclodextrins (CDs)	606:645	Dynamic libraries of cyclodextrins (CDs) could be obtained in wide ranges of pH (5.0-9.0), temperature (5-37 °C), and salinity (up to 7.5 m NaNO3 ), and with high organic solvent content (50 % by volume of ethanol), showing that enzyme-mediated dynamic systems can be robust and not limited to physiological conditions.
32445426	1	24	theme	inherent	231:238	arg1	relevance					251:259	the inherent biological relevance	227:259	the inherent biological relevance of enzymatic transformations	227:288	Enzyme-mediated dynamic combinatorial chemistry combines the concept of thermodynamically controlled covalent self-assembly with the inherent biological relevance of enzymatic transformations.
32445426	0	25	theme	Enzyme-Mediated	22:36	arg1	Libraries					59:67	Enzyme-Mediated Dynamic Cyclodextrin Libraries	22:67	Enzyme-Mediated Dynamic Cyclodextrin Libraries	22:67	Tuning the Outcome of Enzyme-Mediated Dynamic Cyclodextrin Libraries to Enhance Template Effects.
32445426	1	26	theme	enzymatic	264:272	arg1	transformations					274:288	enzymatic transformations	264:288	enzymatic transformations	264:288	Enzyme-mediated dynamic combinatorial chemistry combines the concept of thermodynamically controlled covalent self-assembly with the inherent biological relevance of enzymatic transformations.
32445426	0	27	theme	Libraries	59:67	arg1	Outcome					11:17	the Outcome	7:17	the Outcome of Enzyme-Mediated Dynamic Cyclodextrin Libraries	7:67	Tuning the Outcome of Enzyme-Mediated Dynamic Cyclodextrin Libraries to Enhance Template Effects.
32445426	4	28	theme	dynamic	851:857	arg1	systems					859:865	enzyme-mediated dynamic systems	835:865	enzyme-mediated dynamic systems	835:865	Dynamic libraries of cyclodextrins (CDs) could be obtained in wide ranges of pH (5.0-9.0), temperature (5-37 °C), and salinity (up to 7.5 m NaNO3 ), and with high organic solvent content (50 % by volume of ethanol), showing that enzyme-mediated dynamic systems can be robust and not limited to physiological conditions.
32445426	5	29	theme	due	1126:1128	arg1	target					1119:1124	an otherwise challenging target	1094:1124	an otherwise challenging target due to competition from the structurally similar β-CD	1094:1178	Furthermore, it is demonstrated how strategic choice of reaction conditions can enhance template effects, in this case, to achieve highly selective production of α-CD, an otherwise challenging target due to competition from the structurally similar β-CD.
32445426	5	29	theme	due	1126:1128	arg1	α-CD					1088:1091	α-CD	1088:1091	α-CD	1088:1091	Furthermore, it is demonstrated how strategic choice of reaction conditions can enhance template effects, in this case, to achieve highly selective production of α-CD, an otherwise challenging target due to competition from the structurally similar β-CD.
32445426	5	30	theme	reaction	982:989	arg1	conditions					991:1000	reaction conditions	982:1000	reaction conditions	982:1000	Furthermore, it is demonstrated how strategic choice of reaction conditions can enhance template effects, in this case, to achieve highly selective production of α-CD, an otherwise challenging target due to competition from the structurally similar β-CD.
32445426	4	31	dep	7.5 m	740:744	arg1	to					737:738	to	737:738	to	737:738	Dynamic libraries of cyclodextrins (CDs) could be obtained in wide ranges of pH (5.0-9.0), temperature (5-37 °C), and salinity (up to 7.5 m NaNO3 ), and with high organic solvent content (50 % by volume of ethanol), showing that enzyme-mediated dynamic systems can be robust and not limited to physiological conditions.
32445426	0	32	theme	Cyclodextrin	46:57	arg1	Libraries					59:67	Enzyme-Mediated Dynamic Cyclodextrin Libraries	22:67	Enzyme-Mediated Dynamic Cyclodextrin Libraries	22:67	Tuning the Outcome of Enzyme-Mediated Dynamic Cyclodextrin Libraries to Enhance Template Effects.
32445426	4	33	theme	enzyme-mediated	835:849	arg1	systems					859:865	enzyme-mediated dynamic systems	835:865	enzyme-mediated dynamic systems	835:865	Dynamic libraries of cyclodextrins (CDs) could be obtained in wide ranges of pH (5.0-9.0), temperature (5-37 °C), and salinity (up to 7.5 m NaNO3 ), and with high organic solvent content (50 % by volume of ethanol), showing that enzyme-mediated dynamic systems can be robust and not limited to physiological conditions.
32445426	5	34	theme	conditions	991:1000	arg1	choice					972:977	strategic choice	962:977	strategic choice of reaction conditions	962:1000	Furthermore, it is demonstrated how strategic choice of reaction conditions can enhance template effects, in this case, to achieve highly selective production of α-CD, an otherwise challenging target due to competition from the structurally similar β-CD.
32445426	5	35	theme	strategic	962:970	arg1	choice					972:977	strategic choice	962:977	strategic choice of reaction conditions	962:1000	Furthermore, it is demonstrated how strategic choice of reaction conditions can enhance template effects, in this case, to achieve highly selective production of α-CD, an otherwise challenging target due to competition from the structurally similar β-CD.
32445426	5	36	from	β-CD	1175:1178	arg1	competition					1133:1143	competition	1133:1143	competition from the structurally similar β-CD	1133:1178	Furthermore, it is demonstrated how strategic choice of reaction conditions can enhance template effects, in this case, to achieve highly selective production of α-CD, an otherwise challenging target due to competition from the structurally similar β-CD.
32445426	4	37	theme	high	764:767	arg1	content					785:791	high organic solvent content	764:791	high organic solvent content (50 % by volume of ethanol)	764:819	Dynamic libraries of cyclodextrins (CDs) could be obtained in wide ranges of pH (5.0-9.0), temperature (5-37 °C), and salinity (up to 7.5 m NaNO3 ), and with high organic solvent content (50 % by volume of ethanol), showing that enzyme-mediated dynamic systems can be robust and not limited to physiological conditions.
32445426	4	38	theme	salinity	724:731	arg1	ranges					673:678	wide ranges	668:678	wide ranges of pH (5.0-9.0), temperature (5-37 °C), and salinity (up to 7.5 m NaNO3 )	668:752	Dynamic libraries of cyclodextrins (CDs) could be obtained in wide ranges of pH (5.0-9.0), temperature (5-37 °C), and salinity (up to 7.5 m NaNO3 ), and with high organic solvent content (50 % by volume of ethanol), showing that enzyme-mediated dynamic systems can be robust and not limited to physiological conditions.
32445426	0	39	dep	Enhance	72:78	arg1	Tuning					0:5	Tuning	0:5	Tuning	0:5	Tuning the Outcome of Enzyme-Mediated Dynamic Cyclodextrin Libraries to Enhance Template Effects.
32445426	1	40	theme	transformations	274:288	arg1	relevance					251:259	the inherent biological relevance	227:259	the inherent biological relevance of enzymatic transformations	227:288	Enzyme-mediated dynamic combinatorial chemistry combines the concept of thermodynamically controlled covalent self-assembly with the inherent biological relevance of enzymatic transformations.
32445426	5	41	theme	template	1014:1021	arg1	effects					1023:1029	template effects	1014:1029	template effects	1014:1029	Furthermore, it is demonstrated how strategic choice of reaction conditions can enhance template effects, in this case, to achieve highly selective production of α-CD, an otherwise challenging target due to competition from the structurally similar β-CD.
32445426	2	42	theme	cyclodextrins	319:331	arg1	system					293:298	A system	291:298	A system of interconverting cyclodextrins	291:331	A system of interconverting cyclodextrins has been explored, in which the glycosidic linkage is rendered dynamic by the action of cyclodextrin glucanotransferase (CGTase).
32445426	4	43	dep	content	785:791	arg1	%					797:797	50 %	794:797	50 % by volume of ethanol	794:818	Dynamic libraries of cyclodextrins (CDs) could be obtained in wide ranges of pH (5.0-9.0), temperature (5-37 °C), and salinity (up to 7.5 m NaNO3 ), and with high organic solvent content (50 % by volume of ethanol), showing that enzyme-mediated dynamic systems can be robust and not limited to physiological conditions.
32445426	2	44	theme	interconverting	303:317	arg1	cyclodextrins					319:331	interconverting cyclodextrins	303:331	interconverting cyclodextrins	303:331	A system of interconverting cyclodextrins has been explored, in which the glycosidic linkage is rendered dynamic by the action of cyclodextrin glucanotransferase (CGTase).
32445426	4	45	theme	wide	668:671	arg1	ranges					673:678	wide ranges	668:678	wide ranges of pH (5.0-9.0), temperature (5-37 °C), and salinity (up to 7.5 m NaNO3 )	668:752	Dynamic libraries of cyclodextrins (CDs) could be obtained in wide ranges of pH (5.0-9.0), temperature (5-37 °C), and salinity (up to 7.5 m NaNO3 ), and with high organic solvent content (50 % by volume of ethanol), showing that enzyme-mediated dynamic systems can be robust and not limited to physiological conditions.
32445426	2	46	theme	cyclodextrin	421:432	arg1	CGTase					454:459	CGTase	454:459	CGTase	454:459	A system of interconverting cyclodextrins has been explored, in which the glycosidic linkage is rendered dynamic by the action of cyclodextrin glucanotransferase (CGTase).
32445426	2	46	theme	cyclodextrin	421:432	arg1	glucanotransferase					434:451	cyclodextrin glucanotransferase	421:451	cyclodextrin glucanotransferase (CGTase)	421:460	A system of interconverting cyclodextrins has been explored, in which the glycosidic linkage is rendered dynamic by the action of cyclodextrin glucanotransferase (CGTase).
32445426	5	47	theme	selective	1064:1072	arg1	production					1074:1083	highly selective production	1057:1083	highly selective production of α-CD, an otherwise challenging target due to competition from the structurally similar β-CD	1057:1178	Furthermore, it is demonstrated how strategic choice of reaction conditions can enhance template effects, in this case, to achieve highly selective production of α-CD, an otherwise challenging target due to competition from the structurally similar β-CD.
32445426	4	48	theme	organic	769:775	arg1	content					785:791	high organic solvent content	764:791	high organic solvent content (50 % by volume of ethanol)	764:819	Dynamic libraries of cyclodextrins (CDs) could be obtained in wide ranges of pH (5.0-9.0), temperature (5-37 °C), and salinity (up to 7.5 m NaNO3 ), and with high organic solvent content (50 % by volume of ethanol), showing that enzyme-mediated dynamic systems can be robust and not limited to physiological conditions.
32445426	4	49	theme	physiological	900:912	arg1	conditions					914:923	physiological conditions	900:923	physiological conditions	900:923	Dynamic libraries of cyclodextrins (CDs) could be obtained in wide ranges of pH (5.0-9.0), temperature (5-37 °C), and salinity (up to 7.5 m NaNO3 ), and with high organic solvent content (50 % by volume of ethanol), showing that enzyme-mediated dynamic systems can be robust and not limited to physiological conditions.
33947560	4	0	theme	alginate	849:856	arg1	samples					858:864	the alginate samples	845:864	the alginate samples	845:864	Initially, a preliminary investigation of the raw materials is carried out via rheological and zeta-potential measurements to determine the different grade of polyelectrolyte behaviour of the alginate samples.
33947560	9	1	from	adhesion	1983:1990	arg1	%					2010:2010	the range 15-36%	1995:2010	the range 15-36% with respect to seeded cells	1995:2039	Specifically, polysaccharide samples with an evident polyelectrolyte nature are found to better promote cell viability (i.e., cell adhesion in the range 15-36% with respect to seeded cells) compared to the ones displaying a nearly neutral behaviour (i.e., cell adhesion in the range 5-25% with respect to seeded cells).
33947560	6	2	theme	scanning	1290:1297	arg1	electron					1299:1306	both scanning electron and atomic force microscopy	1285:1334	electron	1299:1306	Subsequently, physically crosslinked alginate nanofibrous mats are first morphologically characterized via both scanning electron and atomic force microscopy, which show a homogenous and defect-free structure, and their biological response is then evaluated.
33947560	9	3	dep	adhesion	2113:2120	arg1	i.e.					2102:2105	i.e.	2102:2105	i.e.	2102:2105	Specifically, polysaccharide samples with an evident polyelectrolyte nature are found to better promote cell viability (i.e., cell adhesion in the range 15-36% with respect to seeded cells) compared to the ones displaying a nearly neutral behaviour (i.e., cell adhesion in the range 5-25% with respect to seeded cells).
33947560	10	4	theme	obtained	2187:2194	arg1	results					2196:2202	the obtained results	2183:2202	the obtained results	2183:2202	Therefore, the obtained results, despite being preliminary, suggest that the alginate type (i.e., molecular structure properties) may play a topical role in conditioning the efficiency of healing patches for bone reparation, but it has a negligible effect in the case of skin regeneration.
33947560	2	5	theme	alginate	302:309	arg1	mass					321:324	alginate molecular mass	302:324	alginate molecular mass	302:324	However, to date, the effect of alginate molecular mass and block composition on the biological response of such systems remains to some extent unclear.
33947560	7	6	theme	cell	1477:1480	arg1	lines					1482:1486	fibroblast and keratinocyte cell lines	1449:1486	lines	1482:1486	Noticeably, fibroblast and keratinocyte cell lines do not show significant differences in terms of cell adhesion on the three mats (i.e., 30-40% and 10-20% with respect to the seeded cells, respectively), with the formers presenting a greater affinity toward the alginate-based nanofibers.
33947560	4	7	theme	polyelectrolyte	816:830	arg1	behaviour					832:840	polyelectrolyte behaviour	816:840	polyelectrolyte behaviour of the alginate samples	816:864	Initially, a preliminary investigation of the raw materials is carried out via rheological and zeta-potential measurements to determine the different grade of polyelectrolyte behaviour of the alginate samples.
33947560	3	8	theme	different	502:510	arg1	features					522:529	different molecular features	502:529	different molecular features	502:529	As such, in the present work, three alginates (i.e., M.pyr, L.hyp, A.nod) with different molecular features are employed to prepare nanofibers whose ability to promote cell adhesion is explored by using both skin and bone cell lines.
33947560	1	9	theme	promising	198:206	arg1	applications					208:219	promising applications	198:219	promising applications in the biomedical and pharmaceutical industries	198:267	Alginate-based electrospun nanofibers prepared via electrospinning technique represent a class of materials with promising applications in the biomedical and pharmaceutical industries.
33947560	9	10	theme	polyelectrolyte	1905:1919	arg1	nature					1921:1926	an evident polyelectrolyte nature	1894:1926	an evident polyelectrolyte nature	1894:1926	Specifically, polysaccharide samples with an evident polyelectrolyte nature are found to better promote cell viability (i.e., cell adhesion in the range 15-36% with respect to seeded cells) compared to the ones displaying a nearly neutral behaviour (i.e., cell adhesion in the range 5-25% with respect to seeded cells).
33947560	10	11	theme	bone	2380:2383	arg1	reparation					2385:2394	bone reparation	2380:2394	bone reparation	2380:2394	Therefore, the obtained results, despite being preliminary, suggest that the alginate type (i.e., molecular structure properties) may play a topical role in conditioning the efficiency of healing patches for bone reparation, but it has a negligible effect in the case of skin regeneration.
33947560	6	12	link	crosslinked	1203:1213	arg1	mats					1236:1239	physically crosslinked alginate nanofibrous mats	1192:1239	physically crosslinked alginate nanofibrous mats	1192:1239	Subsequently, physically crosslinked alginate nanofibrous mats are first morphologically characterized via both scanning electron and atomic force microscopy, which show a homogenous and defect-free structure, and their biological response is then evaluated.
33947560	9	13	from	adhesion	2113:2120	arg1	%					2139:2139	the range 5-25%	2125:2139	the range 5-25% with respect to seeded cells	2125:2168	Specifically, polysaccharide samples with an evident polyelectrolyte nature are found to better promote cell viability (i.e., cell adhesion in the range 15-36% with respect to seeded cells) compared to the ones displaying a nearly neutral behaviour (i.e., cell adhesion in the range 5-25% with respect to seeded cells).
33947560	2	14	theme	block	330:334	arg1	composition					336:346	block composition	330:346	block composition	330:346	However, to date, the effect of alginate molecular mass and block composition on the biological response of such systems remains to some extent unclear.
33947560	7	15	theme	seeded	1613:1618	arg1	cells					1620:1624	the seeded cells	1609:1624	the seeded cells	1609:1624	Noticeably, fibroblast and keratinocyte cell lines do not show significant differences in terms of cell adhesion on the three mats (i.e., 30-40% and 10-20% with respect to the seeded cells, respectively), with the formers presenting a greater affinity toward the alginate-based nanofibers.
33947560	10	16	theme	alginate	2249:2256	arg1	properties					2290:2299	properties	2290:2299	properties	2290:2299	Therefore, the obtained results, despite being preliminary, suggest that the alginate type (i.e., molecular structure properties) may play a topical role in conditioning the efficiency of healing patches for bone reparation, but it has a negligible effect in the case of skin regeneration.
33947560	10	16	theme	alginate	2249:2256	arg1	type					2258:2261	the alginate type	2245:2261	the alginate type (i.e., molecular structure properties)	2245:2300	Therefore, the obtained results, despite being preliminary, suggest that the alginate type (i.e., molecular structure properties) may play a topical role in conditioning the efficiency of healing patches for bone reparation, but it has a negligible effect in the case of skin regeneration.
33947560	5	17	theme	moieties	1043:1050	arg1	chains					1004:1009	long chains	999:1009	long chains	999:1009	Specifically, both the molecular mass and block composition are found to be important factors affecting the alginate response, with long chains and a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration).
33947560	5	17	theme	moieties	1043:1050	arg1	predominance					1017:1028	a predominance	1015:1028	a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration)	1015:1175	Specifically, both the molecular mass and block composition are found to be important factors affecting the alginate response, with long chains and a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration).
33947560	2	18	theme	mass	321:324	arg1	effect					292:297	the effect	288:297	the effect of alginate molecular mass and block composition on the biological response of such systems	288:389	However, to date, the effect of alginate molecular mass and block composition on the biological response of such systems remains to some extent unclear.
33947560	1	19	theme	biomedical	228:237	arg1	industries					258:267	the biomedical and pharmaceutical industries	224:267	the biomedical and pharmaceutical industries	224:267	Alginate-based electrospun nanofibers prepared via electrospinning technique represent a class of materials with promising applications in the biomedical and pharmaceutical industries.
33947560	6	20	theme	nanofibrous	1224:1234	arg1	mats					1236:1239	physically crosslinked alginate nanofibrous mats	1192:1239	physically crosslinked alginate nanofibrous mats	1192:1239	Subsequently, physically crosslinked alginate nanofibrous mats are first morphologically characterized via both scanning electron and atomic force microscopy, which show a homogenous and defect-free structure, and their biological response is then evaluated.
33947560	9	21	theme	range	2129:2133	arg1	%					2139:2139	the range 5-25%	2125:2139	the range 5-25% with respect to seeded cells	2125:2168	Specifically, polysaccharide samples with an evident polyelectrolyte nature are found to better promote cell viability (i.e., cell adhesion in the range 15-36% with respect to seeded cells) compared to the ones displaying a nearly neutral behaviour (i.e., cell adhesion in the range 5-25% with respect to seeded cells).
33947560	1	22	theme	pharmaceutical	243:256	arg1	industries					258:267	the biomedical and pharmaceutical industries	224:267	the biomedical and pharmaceutical industries	224:267	Alginate-based electrospun nanofibers prepared via electrospinning technique represent a class of materials with promising applications in the biomedical and pharmaceutical industries.
33947560	6	23	theme	crosslinked	1203:1213	arg1	mats					1236:1239	physically crosslinked alginate nanofibrous mats	1192:1239	physically crosslinked alginate nanofibrous mats	1192:1239	Subsequently, physically crosslinked alginate nanofibrous mats are first morphologically characterized via both scanning electron and atomic force microscopy, which show a homogenous and defect-free structure, and their biological response is then evaluated.
33947560	4	24	theme	preliminary	670:680	arg1	investigation					682:694	a preliminary investigation	668:694	a preliminary investigation of the raw materials	668:715	Initially, a preliminary investigation of the raw materials is carried out via rheological and zeta-potential measurements to determine the different grade of polyelectrolyte behaviour of the alginate samples.
33947560	6	25	theme	atomic	1312:1317	arg1	microscopy					1325:1334	both scanning electron and atomic force microscopy	1285:1334	microscopy	1325:1334	Subsequently, physically crosslinked alginate nanofibrous mats are first morphologically characterized via both scanning electron and atomic force microscopy, which show a homogenous and defect-free structure, and their biological response is then evaluated.
33947560	7	26	theme	alginate-based	1700:1713	arg1	nanofibers					1715:1724	the alginate-based nanofibers	1696:1724	the alginate-based nanofibers	1696:1724	Noticeably, fibroblast and keratinocyte cell lines do not show significant differences in terms of cell adhesion on the three mats (i.e., 30-40% and 10-20% with respect to the seeded cells, respectively), with the formers presenting a greater affinity toward the alginate-based nanofibers.
33947560	3	27	theme	molecular	512:520	arg1	features					522:529	different molecular features	502:529	different molecular features	502:529	As such, in the present work, three alginates (i.e., M.pyr, L.hyp, A.nod) with different molecular features are employed to prepare nanofibers whose ability to promote cell adhesion is explored by using both skin and bone cell lines.
33947560	8	28	theme	investigated	1748:1759	arg1	cells					1772:1776	both the investigated osteoblast cells	1739:1776	both the investigated osteoblast cells	1739:1776	Conversely, both the investigated osteoblast cells are characterized by a distinct behaviour depending on the alginate type.
33947560	5	29	theme	block	909:913	arg1	composition					915:925	block composition	909:925	block composition	909:925	Specifically, both the molecular mass and block composition are found to be important factors affecting the alginate response, with long chains and a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration).
33947560	10	30	theme	regeneration	2448:2459	arg1	case					2435:2438	the case	2431:2438	the case of skin regeneration	2431:2459	Therefore, the obtained results, despite being preliminary, suggest that the alginate type (i.e., molecular structure properties) may play a topical role in conditioning the efficiency of healing patches for bone reparation, but it has a negligible effect in the case of skin regeneration.
33947560	9	31	theme	seeded	2157:2162	arg1	cells					2164:2168	seeded cells	2157:2168	seeded cells	2157:2168	Specifically, polysaccharide samples with an evident polyelectrolyte nature are found to better promote cell viability (i.e., cell adhesion in the range 15-36% with respect to seeded cells) compared to the ones displaying a nearly neutral behaviour (i.e., cell adhesion in the range 5-25% with respect to seeded cells).
33947560	0	32	theme	membrane	61:68	arg1	adhesion					75:82	electrospun membrane cell adhesion	49:82	electrospun membrane cell adhesion	49:82	Effect of sodium alginate molecular structure on electrospun membrane cell adhesion.
33947560	3	33	theme	cell	591:594	arg1	adhesion					596:603	cell adhesion	591:603	cell adhesion	591:603	As such, in the present work, three alginates (i.e., M.pyr, L.hyp, A.nod) with different molecular features are employed to prepare nanofibers whose ability to promote cell adhesion is explored by using both skin and bone cell lines.
33947560	7	34	theme	greater	1672:1678	arg1	affinity					1680:1687	a greater affinity	1670:1687	a greater affinity toward the alginate-based nanofibers	1670:1724	Noticeably, fibroblast and keratinocyte cell lines do not show significant differences in terms of cell adhesion on the three mats (i.e., 30-40% and 10-20% with respect to the seeded cells, respectively), with the formers presenting a greater affinity toward the alginate-based nanofibers.
33947560	9	35	theme	cell	1978:1981	arg1	adhesion					1983:1990	cell adhesion	1978:1990	cell adhesion	1978:1990	Specifically, polysaccharide samples with an evident polyelectrolyte nature are found to better promote cell viability (i.e., cell adhesion in the range 15-36% with respect to seeded cells) compared to the ones displaying a nearly neutral behaviour (i.e., cell adhesion in the range 5-25% with respect to seeded cells).
33947560	5	36	theme	lower	1102:1106	arg1	dependence					1108:1117	lower dependence	1102:1117	lower dependence of the solution viscosity upon the polymer concentration	1102:1174	Specifically, both the molecular mass and block composition are found to be important factors affecting the alginate response, with long chains and a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration).
33947560	1	37	with	materials	183:191	arg1	applications					208:219	promising applications	198:219	promising applications in the biomedical and pharmaceutical industries	198:267	Alginate-based electrospun nanofibers prepared via electrospinning technique represent a class of materials with promising applications in the biomedical and pharmaceutical industries.
33947560	1	38	theme	materials	183:191	arg1	class					174:178	a class	172:178	a class of materials with promising applications in the biomedical and pharmaceutical industries	172:267	Alginate-based electrospun nanofibers prepared via electrospinning technique represent a class of materials with promising applications in the biomedical and pharmaceutical industries.
33947560	9	39	dep	ones	2058:2061	arg1	displaying					2063:2072	displaying	2063:2072	displaying a nearly neutral behaviour (i.e., cell adhesion in the range 5-25% with respect to seeded cells)	2063:2169	Specifically, polysaccharide samples with an evident polyelectrolyte nature are found to better promote cell viability (i.e., cell adhesion in the range 15-36% with respect to seeded cells) compared to the ones displaying a nearly neutral behaviour (i.e., cell adhesion in the range 5-25% with respect to seeded cells).
33947560	10	40	theme	topical	2313:2319	arg1	role					2321:2324	a topical role	2311:2324	a topical role	2311:2324	Therefore, the obtained results, despite being preliminary, suggest that the alginate type (i.e., molecular structure properties) may play a topical role in conditioning the efficiency of healing patches for bone reparation, but it has a negligible effect in the case of skin regeneration.
33947560	0	41	from	Effect	0:5	arg1	adhesion					75:82	electrospun membrane cell adhesion	49:82	electrospun membrane cell adhesion	49:82	Effect of sodium alginate molecular structure on electrospun membrane cell adhesion.
33947560	1	42	from	applications	208:219	arg1	industries					258:267	the biomedical and pharmaceutical industries	224:267	the biomedical and pharmaceutical industries	224:267	Alginate-based electrospun nanofibers prepared via electrospinning technique represent a class of materials with promising applications in the biomedical and pharmaceutical industries.
33947560	5	43	theme	marked	1065:1070	arg1	nature					1088:1093	a marked polyelectrolyte nature	1063:1093	a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration)	1063:1175	Specifically, both the molecular mass and block composition are found to be important factors affecting the alginate response, with long chains and a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration).
33947560	5	44	theme	important	943:951	arg1	mass					900:903	the molecular mass	886:903	the molecular mass	886:903	Specifically, both the molecular mass and block composition are found to be important factors affecting the alginate response, with long chains and a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration).
33947560	5	44	theme	important	943:951	arg1	factors					953:959	important factors	943:959	important factors affecting the alginate response	943:991	Specifically, both the molecular mass and block composition are found to be important factors affecting the alginate response, with long chains and a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration).
33947560	9	45	theme	range	1999:2003	arg1	%					2010:2010	the range 15-36%	1995:2010	the range 15-36% with respect to seeded cells	1995:2039	Specifically, polysaccharide samples with an evident polyelectrolyte nature are found to better promote cell viability (i.e., cell adhesion in the range 15-36% with respect to seeded cells) compared to the ones displaying a nearly neutral behaviour (i.e., cell adhesion in the range 5-25% with respect to seeded cells).
33947560	4	46	theme	rheological	736:746	arg1	measurements					767:778	rheological and zeta-potential measurements	736:778	rheological and zeta-potential measurements	736:778	Initially, a preliminary investigation of the raw materials is carried out via rheological and zeta-potential measurements to determine the different grade of polyelectrolyte behaviour of the alginate samples.
33947560	3	47	theme	present	439:445	arg1	work					447:450	the present work	435:450	the present work	435:450	As such, in the present work, three alginates (i.e., M.pyr, L.hyp, A.nod) with different molecular features are employed to prepare nanofibers whose ability to promote cell adhesion is explored by using both skin and bone cell lines.
33947560	4	48	theme	raw	703:705	arg1	materials					707:715	the raw materials	699:715	the raw materials	699:715	Initially, a preliminary investigation of the raw materials is carried out via rheological and zeta-potential measurements to determine the different grade of polyelectrolyte behaviour of the alginate samples.
33947560	1	49	theme	electrospun	100:110	arg1	nanofibers					112:121	Alginate-based electrospun nanofibers	85:121	Alginate-based electrospun nanofibers prepared via electrospinning technique	85:160	Alginate-based electrospun nanofibers prepared via electrospinning technique represent a class of materials with promising applications in the biomedical and pharmaceutical industries.
33947560	5	50	dep	dependence	1108:1117	arg1	i.e.					1096:1099	i.e.	1096:1099	i.e.	1096:1099	Specifically, both the molecular mass and block composition are found to be important factors affecting the alginate response, with long chains and a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration).
33947560	0	51	theme	sodium	10:15	arg1	structure					36:44	sodium alginate molecular structure	10:44	sodium alginate molecular structure	10:44	Effect of sodium alginate molecular structure on electrospun membrane cell adhesion.
33947560	5	52	theme	polymer	1154:1160	arg1	concentration					1162:1174	the polymer concentration	1150:1174	the polymer concentration	1150:1174	Specifically, both the molecular mass and block composition are found to be important factors affecting the alginate response, with long chains and a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration).
33947560	7	53	theme	adhesion	1541:1548	arg1	terms					1527:1531	terms	1527:1531	terms of cell adhesion	1527:1548	Noticeably, fibroblast and keratinocyte cell lines do not show significant differences in terms of cell adhesion on the three mats (i.e., 30-40% and 10-20% with respect to the seeded cells, respectively), with the formers presenting a greater affinity toward the alginate-based nanofibers.
33947560	10	54	theme	patches	2368:2374	arg1	efficiency					2346:2355	the efficiency	2342:2355	the efficiency of healing patches for bone reparation	2342:2394	Therefore, the obtained results, despite being preliminary, suggest that the alginate type (i.e., molecular structure properties) may play a topical role in conditioning the efficiency of healing patches for bone reparation, but it has a negligible effect in the case of skin regeneration.
33947560	9	55	theme	seeded	2028:2033	arg1	cells					2035:2039	seeded cells	2028:2039	seeded cells	2028:2039	Specifically, polysaccharide samples with an evident polyelectrolyte nature are found to better promote cell viability (i.e., cell adhesion in the range 15-36% with respect to seeded cells) compared to the ones displaying a nearly neutral behaviour (i.e., cell adhesion in the range 5-25% with respect to seeded cells).
33947560	0	56	theme	molecular	26:34	arg1	structure					36:44	sodium alginate molecular structure	10:44	sodium alginate molecular structure	10:44	Effect of sodium alginate molecular structure on electrospun membrane cell adhesion.
33947560	5	57	theme	long	999:1002	arg1	chains					1004:1009	long chains	999:1009	long chains	999:1009	Specifically, both the molecular mass and block composition are found to be important factors affecting the alginate response, with long chains and a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration).
33947560	6	58	theme	biological	1398:1407	arg1	response					1409:1416	their biological response	1392:1416	their biological response	1392:1416	Subsequently, physically crosslinked alginate nanofibrous mats are first morphologically characterized via both scanning electron and atomic force microscopy, which show a homogenous and defect-free structure, and their biological response is then evaluated.
33947560	5	59	theme	solution	1126:1133	arg1	viscosity					1135:1143	the solution viscosity	1122:1143	the solution viscosity	1122:1143	Specifically, both the molecular mass and block composition are found to be important factors affecting the alginate response, with long chains and a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration).
33947560	3	60	theme	cell	645:648	arg1	lines					650:654	both skin and bone cell lines	626:654	lines	650:654	As such, in the present work, three alginates (i.e., M.pyr, L.hyp, A.nod) with different molecular features are employed to prepare nanofibers whose ability to promote cell adhesion is explored by using both skin and bone cell lines.
33947560	4	61	theme	zeta-potential	752:765	arg1	measurements					767:778	rheological and zeta-potential measurements	736:778	rheological and zeta-potential measurements	736:778	Initially, a preliminary investigation of the raw materials is carried out via rheological and zeta-potential measurements to determine the different grade of polyelectrolyte behaviour of the alginate samples.
33947560	7	62	theme	significant	1500:1510	arg1	differences					1512:1522	significant differences	1500:1522	significant differences in terms of cell adhesion	1500:1548	Noticeably, fibroblast and keratinocyte cell lines do not show significant differences in terms of cell adhesion on the three mats (i.e., 30-40% and 10-20% with respect to the seeded cells, respectively), with the formers presenting a greater affinity toward the alginate-based nanofibers.
33947560	5	63	theme	alginate	975:982	arg1	response					984:991	the alginate response	971:991	the alginate response	971:991	Specifically, both the molecular mass and block composition are found to be important factors affecting the alginate response, with long chains and a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration).
33947560	7	64	from	differences	1512:1522	arg1	terms					1527:1531	terms	1527:1531	terms of cell adhesion	1527:1548	Noticeably, fibroblast and keratinocyte cell lines do not show significant differences in terms of cell adhesion on the three mats (i.e., 30-40% and 10-20% with respect to the seeded cells, respectively), with the formers presenting a greater affinity toward the alginate-based nanofibers.
33947560	9	65	with	samples	1881:1887	arg1	nature					1921:1926	an evident polyelectrolyte nature	1894:1926	an evident polyelectrolyte nature	1894:1926	Specifically, polysaccharide samples with an evident polyelectrolyte nature are found to better promote cell viability (i.e., cell adhesion in the range 15-36% with respect to seeded cells) compared to the ones displaying a nearly neutral behaviour (i.e., cell adhesion in the range 5-25% with respect to seeded cells).
33947560	8	66	theme	alginate	1837:1844	arg1	type					1846:1849	the alginate type	1833:1849	the alginate type	1833:1849	Conversely, both the investigated osteoblast cells are characterized by a distinct behaviour depending on the alginate type.
33947560	2	67	theme	such	378:381	arg1	systems					383:389	such systems	378:389	such systems	378:389	However, to date, the effect of alginate molecular mass and block composition on the biological response of such systems remains to some extent unclear.
33947560	9	68	theme	polysaccharide	1866:1879	arg1	samples					1881:1887	polysaccharide samples	1866:1887	polysaccharide samples with an evident polyelectrolyte nature	1866:1926	Specifically, polysaccharide samples with an evident polyelectrolyte nature are found to better promote cell viability (i.e., cell adhesion in the range 15-36% with respect to seeded cells) compared to the ones displaying a nearly neutral behaviour (i.e., cell adhesion in the range 5-25% with respect to seeded cells).
33947560	9	69	theme	neutral	2083:2089	arg1	behaviour					2091:2099	a nearly neutral behaviour	2074:2099	a nearly neutral behaviour	2074:2099	Specifically, polysaccharide samples with an evident polyelectrolyte nature are found to better promote cell viability (i.e., cell adhesion in the range 15-36% with respect to seeded cells) compared to the ones displaying a nearly neutral behaviour (i.e., cell adhesion in the range 5-25% with respect to seeded cells).
33947560	2	70	theme	molecular	311:319	arg1	mass					321:324	alginate molecular mass	302:324	alginate molecular mass	302:324	However, to date, the effect of alginate molecular mass and block composition on the biological response of such systems remains to some extent unclear.
33947560	7	71	theme	keratinocyte	1464:1475	arg1	lines					1482:1486	fibroblast and keratinocyte cell lines	1449:1486	lines	1482:1486	Noticeably, fibroblast and keratinocyte cell lines do not show significant differences in terms of cell adhesion on the three mats (i.e., 30-40% and 10-20% with respect to the seeded cells, respectively), with the formers presenting a greater affinity toward the alginate-based nanofibers.
33947560	4	72	theme	samples	858:864	arg1	behaviour					832:840	polyelectrolyte behaviour	816:840	polyelectrolyte behaviour of the alginate samples	816:864	Initially, a preliminary investigation of the raw materials is carried out via rheological and zeta-potential measurements to determine the different grade of polyelectrolyte behaviour of the alginate samples.
33947560	7	73	theme	fibroblast	1449:1458	arg1	lines					1482:1486	fibroblast and keratinocyte cell lines	1449:1486	lines	1482:1486	Noticeably, fibroblast and keratinocyte cell lines do not show significant differences in terms of cell adhesion on the three mats (i.e., 30-40% and 10-20% with respect to the seeded cells, respectively), with the formers presenting a greater affinity toward the alginate-based nanofibers.
33947560	9	74	theme	evident	1897:1903	arg1	nature					1921:1926	an evident polyelectrolyte nature	1894:1926	an evident polyelectrolyte nature	1894:1926	Specifically, polysaccharide samples with an evident polyelectrolyte nature are found to better promote cell viability (i.e., cell adhesion in the range 15-36% with respect to seeded cells) compared to the ones displaying a nearly neutral behaviour (i.e., cell adhesion in the range 5-25% with respect to seeded cells).
33947560	9	75	theme	cell	2108:2111	arg1	adhesion					2113:2120	cell adhesion	2108:2120	cell adhesion	2108:2120	Specifically, polysaccharide samples with an evident polyelectrolyte nature are found to better promote cell viability (i.e., cell adhesion in the range 15-36% with respect to seeded cells) compared to the ones displaying a nearly neutral behaviour (i.e., cell adhesion in the range 5-25% with respect to seeded cells).
33947560	2	76	theme	composition	336:346	arg1	effect					292:297	the effect	288:297	the effect of alginate molecular mass and block composition on the biological response of such systems	288:389	However, to date, the effect of alginate molecular mass and block composition on the biological response of such systems remains to some extent unclear.
33947560	9	77	dep	promote	1948:1954	arg1	adhesion					1983:1990	cell adhesion	1978:1990	cell adhesion	1978:1990	Specifically, polysaccharide samples with an evident polyelectrolyte nature are found to better promote cell viability (i.e., cell adhesion in the range 15-36% with respect to seeded cells) compared to the ones displaying a nearly neutral behaviour (i.e., cell adhesion in the range 5-25% with respect to seeded cells).
33947560	3	78	dep	ability	572:578	arg1	promote					583:589	promote	583:589	to promote cell adhesion	580:603	As such, in the present work, three alginates (i.e., M.pyr, L.hyp, A.nod) with different molecular features are employed to prepare nanofibers whose ability to promote cell adhesion is explored by using both skin and bone cell lines.
33947560	4	79	theme	behaviour	832:840	arg1	grade					807:811	the different grade	793:811	the different grade of polyelectrolyte behaviour of the alginate samples	793:864	Initially, a preliminary investigation of the raw materials is carried out via rheological and zeta-potential measurements to determine the different grade of polyelectrolyte behaviour of the alginate samples.
33947560	9	80	dep	displaying	2063:2072	arg1	adhesion					2113:2120	cell adhesion	2108:2120	cell adhesion	2108:2120	Specifically, polysaccharide samples with an evident polyelectrolyte nature are found to better promote cell viability (i.e., cell adhesion in the range 15-36% with respect to seeded cells) compared to the ones displaying a nearly neutral behaviour (i.e., cell adhesion in the range 5-25% with respect to seeded cells).
33947560	5	81	with	factors	953:959	arg1	chains					1004:1009	long chains	999:1009	long chains	999:1009	Specifically, both the molecular mass and block composition are found to be important factors affecting the alginate response, with long chains and a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration).
33947560	5	81	with	factors	953:959	arg1	predominance					1017:1028	a predominance	1015:1028	a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration)	1015:1175	Specifically, both the molecular mass and block composition are found to be important factors affecting the alginate response, with long chains and a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration).
33947560	3	82	with	alginates	459:467	arg1	features					522:529	different molecular features	502:529	different molecular features	502:529	As such, in the present work, three alginates (i.e., M.pyr, L.hyp, A.nod) with different molecular features are employed to prepare nanofibers whose ability to promote cell adhesion is explored by using both skin and bone cell lines.
33947560	6	83	theme	alginate	1215:1222	arg1	mats					1236:1239	physically crosslinked alginate nanofibrous mats	1192:1239	physically crosslinked alginate nanofibrous mats	1192:1239	Subsequently, physically crosslinked alginate nanofibrous mats are first morphologically characterized via both scanning electron and atomic force microscopy, which show a homogenous and defect-free structure, and their biological response is then evaluated.
33947560	6	84	theme	force	1319:1323	arg1	microscopy					1325:1334	both scanning electron and atomic force microscopy	1285:1334	microscopy	1325:1334	Subsequently, physically crosslinked alginate nanofibrous mats are first morphologically characterized via both scanning electron and atomic force microscopy, which show a homogenous and defect-free structure, and their biological response is then evaluated.
33947560	7	85	dep	%	1580:1580	arg1	i.e.					1569:1572	i.e.	1569:1572	i.e.	1569:1572	Noticeably, fibroblast and keratinocyte cell lines do not show significant differences in terms of cell adhesion on the three mats (i.e., 30-40% and 10-20% with respect to the seeded cells, respectively), with the formers presenting a greater affinity toward the alginate-based nanofibers.
33947560	5	86	theme	molecular	890:898	arg1	mass					900:903	the molecular mass	886:903	the molecular mass	886:903	Specifically, both the molecular mass and block composition are found to be important factors affecting the alginate response, with long chains and a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration).
33947560	5	86	theme	molecular	890:898	arg1	factors					953:959	important factors	943:959	important factors affecting the alginate response	943:991	Specifically, both the molecular mass and block composition are found to be important factors affecting the alginate response, with long chains and a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration).
33947560	9	87	theme	cell	1956:1959	arg1	viability					1961:1969	cell viability	1956:1969	cell viability	1956:1969	Specifically, polysaccharide samples with an evident polyelectrolyte nature are found to better promote cell viability (i.e., cell adhesion in the range 15-36% with respect to seeded cells) compared to the ones displaying a nearly neutral behaviour (i.e., cell adhesion in the range 5-25% with respect to seeded cells).
33947560	2	88	from	effect	292:297	arg1	response					366:373	the biological response	351:373	the biological response of such systems	351:389	However, to date, the effect of alginate molecular mass and block composition on the biological response of such systems remains to some extent unclear.
33947560	10	89	theme	negligible	2410:2419	arg1	effect					2421:2426	a negligible effect	2408:2426	a negligible effect	2408:2426	Therefore, the obtained results, despite being preliminary, suggest that the alginate type (i.e., molecular structure properties) may play a topical role in conditioning the efficiency of healing patches for bone reparation, but it has a negligible effect in the case of skin regeneration.
33947560	0	90	theme	cell	70:73	arg1	adhesion					75:82	electrospun membrane cell adhesion	49:82	electrospun membrane cell adhesion	49:82	Effect of sodium alginate molecular structure on electrospun membrane cell adhesion.
33947560	5	91	theme	guluronic	1033:1041	arg1	moieties					1043:1050	guluronic moieties	1033:1050	guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration)	1033:1175	Specifically, both the molecular mass and block composition are found to be important factors affecting the alginate response, with long chains and a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration).
33947560	10	92	theme	skin	2443:2446	arg1	regeneration					2448:2459	skin regeneration	2443:2459	skin regeneration	2443:2459	Therefore, the obtained results, despite being preliminary, suggest that the alginate type (i.e., molecular structure properties) may play a topical role in conditioning the efficiency of healing patches for bone reparation, but it has a negligible effect in the case of skin regeneration.
33947560	8	93	theme	osteoblast	1761:1770	arg1	cells					1772:1776	both the investigated osteoblast cells	1739:1776	both the investigated osteoblast cells	1739:1776	Conversely, both the investigated osteoblast cells are characterized by a distinct behaviour depending on the alginate type.
33947560	10	94	theme	molecular	2270:2278	arg1	structure					2280:2288	molecular structure	2270:2288	molecular structure	2270:2288	Therefore, the obtained results, despite being preliminary, suggest that the alginate type (i.e., molecular structure properties) may play a topical role in conditioning the efficiency of healing patches for bone reparation, but it has a negligible effect in the case of skin regeneration.
33947560	6	95	theme	defect-free	1365:1375	arg1	structure					1377:1385	a homogenous and defect-free structure	1348:1385	a homogenous and defect-free structure	1348:1385	Subsequently, physically crosslinked alginate nanofibrous mats are first morphologically characterized via both scanning electron and atomic force microscopy, which show a homogenous and defect-free structure, and their biological response is then evaluated.
33947560	6	96	theme	homogenous	1350:1359	arg1	structure					1377:1385	a homogenous and defect-free structure	1348:1385	a homogenous and defect-free structure	1348:1385	Subsequently, physically crosslinked alginate nanofibrous mats are first morphologically characterized via both scanning electron and atomic force microscopy, which show a homogenous and defect-free structure, and their biological response is then evaluated.
33947560	5	97	theme	polyelectrolyte	1072:1086	arg1	nature					1088:1093	a marked polyelectrolyte nature	1063:1093	a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration)	1063:1175	Specifically, both the molecular mass and block composition are found to be important factors affecting the alginate response, with long chains and a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration).
33947560	10	98	contain	has	2404:2406	arg1	it					2401:2402	it	2401:2402	it	2401:2402	Therefore, the obtained results, despite being preliminary, suggest that the alginate type (i.e., molecular structure properties) may play a topical role in conditioning the efficiency of healing patches for bone reparation, but it has a negligible effect in the case of skin regeneration.
33947560	10	98	contain	has	2404:2406	arg2	effect					2421:2426	a negligible effect	2408:2426	a negligible effect	2408:2426	Therefore, the obtained results, despite being preliminary, suggest that the alginate type (i.e., molecular structure properties) may play a topical role in conditioning the efficiency of healing patches for bone reparation, but it has a negligible effect in the case of skin regeneration.
33947560	8	99	theme	distinct	1801:1808	arg1	behaviour					1810:1818	a distinct behaviour	1799:1818	a distinct behaviour depending on the alginate type	1799:1849	Conversely, both the investigated osteoblast cells are characterized by a distinct behaviour depending on the alginate type.
33947560	1	100	theme	Alginate-based	85:98	arg1	nanofibers					112:121	Alginate-based electrospun nanofibers	85:121	Alginate-based electrospun nanofibers prepared via electrospinning technique	85:160	Alginate-based electrospun nanofibers prepared via electrospinning technique represent a class of materials with promising applications in the biomedical and pharmaceutical industries.
33947560	0	101	theme	alginate	17:24	arg1	structure					36:44	sodium alginate molecular structure	10:44	sodium alginate molecular structure	10:44	Effect of sodium alginate molecular structure on electrospun membrane cell adhesion.
33947560	3	102	dep	A.nod	490:494	arg1	i.e.					470:473	i.e.	470:473	i.e.	470:473	As such, in the present work, three alginates (i.e., M.pyr, L.hyp, A.nod) with different molecular features are employed to prepare nanofibers whose ability to promote cell adhesion is explored by using both skin and bone cell lines.
33947560	4	103	theme	materials	707:715	arg1	investigation					682:694	a preliminary investigation	668:694	a preliminary investigation of the raw materials	668:715	Initially, a preliminary investigation of the raw materials is carried out via rheological and zeta-potential measurements to determine the different grade of polyelectrolyte behaviour of the alginate samples.
33947560	0	104	theme	structure	36:44	arg1	Effect					0:5	Effect	0:5	Effect of sodium alginate molecular structure on electrospun membrane cell adhesion.	0:83	Effect of sodium alginate molecular structure on electrospun membrane cell adhesion.
33947560	10	105	theme	healing	2360:2366	arg1	patches					2368:2374	healing patches	2360:2374	healing patches	2360:2374	Therefore, the obtained results, despite being preliminary, suggest that the alginate type (i.e., molecular structure properties) may play a topical role in conditioning the efficiency of healing patches for bone reparation, but it has a negligible effect in the case of skin regeneration.
33947560	0	106	theme	electrospun	49:59	arg1	adhesion					75:82	electrospun membrane cell adhesion	49:82	electrospun membrane cell adhesion	49:82	Effect of sodium alginate molecular structure on electrospun membrane cell adhesion.
33947560	10	107	dep	structure	2280:2288	arg1	i.e.					2264:2267	i.e.	2264:2267	i.e.	2264:2267	Therefore, the obtained results, despite being preliminary, suggest that the alginate type (i.e., molecular structure properties) may play a topical role in conditioning the efficiency of healing patches for bone reparation, but it has a negligible effect in the case of skin regeneration.
33947560	7	108	theme	cell	1536:1539	arg1	adhesion					1541:1548	cell adhesion	1536:1548	cell adhesion	1536:1548	Noticeably, fibroblast and keratinocyte cell lines do not show significant differences in terms of cell adhesion on the three mats (i.e., 30-40% and 10-20% with respect to the seeded cells, respectively), with the formers presenting a greater affinity toward the alginate-based nanofibers.
33947560	3	109	theme	bone	640:643	arg1	lines					650:654	both skin and bone cell lines	626:654	lines	650:654	As such, in the present work, three alginates (i.e., M.pyr, L.hyp, A.nod) with different molecular features are employed to prepare nanofibers whose ability to promote cell adhesion is explored by using both skin and bone cell lines.
33947560	4	110	theme	different	797:805	arg1	grade					807:811	the different grade	793:811	the different grade of polyelectrolyte behaviour of the alginate samples	793:864	Initially, a preliminary investigation of the raw materials is carried out via rheological and zeta-potential measurements to determine the different grade of polyelectrolyte behaviour of the alginate samples.
33947560	2	111	theme	biological	355:364	arg1	response					366:373	the biological response	351:373	the biological response of such systems	351:389	However, to date, the effect of alginate molecular mass and block composition on the biological response of such systems remains to some extent unclear.
33947560	5	112	theme	viscosity	1135:1143	arg1	dependence					1108:1117	lower dependence	1102:1117	lower dependence of the solution viscosity upon the polymer concentration	1102:1174	Specifically, both the molecular mass and block composition are found to be important factors affecting the alginate response, with long chains and a predominance of guluronic moieties leading to a marked polyelectrolyte nature (i.e., lower dependence of the solution viscosity upon the polymer concentration).
33947560	9	113	dep	adhesion	1983:1990	arg1	i.e.					1972:1975	i.e.	1972:1975	i.e.	1972:1975	Specifically, polysaccharide samples with an evident polyelectrolyte nature are found to better promote cell viability (i.e., cell adhesion in the range 15-36% with respect to seeded cells) compared to the ones displaying a nearly neutral behaviour (i.e., cell adhesion in the range 5-25% with respect to seeded cells).
33947560	2	114	theme	systems	383:389	arg1	response					366:373	the biological response	351:373	the biological response of such systems	351:389	However, to date, the effect of alginate molecular mass and block composition on the biological response of such systems remains to some extent unclear.
33137387	5	0	theme	different	849:857	arg1	buffers					862:868	different pH buffers	849:868	different pH buffers	849:868	The color indicator film changed clearly from reddish-pink to pale peach and finally to yellow when exposed to different pH buffers.
33137387	1	1	theme	indicator	176:184	arg1	film					186:189	A new pH-responsive color indicator film	150:189	A new pH-responsive color indicator film	150:189	A new pH-responsive color indicator film was prepared by blending barberry anthocyanin (BA) with methylcellulose (MC)/chitosan nanofiber (ChNF) composite film.
33137387	8	2	theme	pH-sensing	1056:1065	arg1	film					1083:1086	the pH-sensing color indicator film	1052:1086	the pH-sensing color indicator film	1052:1086	Therefore, the pH-sensing color indicator film can be used as a smart indicator for real-time freshness monitoring of meat and seafood products.
33137387	8	2	theme	pH-sensing	1056:1065	arg1	indicator					1111:1119	a smart indicator	1103:1119	a smart indicator for real-time freshness monitoring of meat and seafood products	1103:1183	Therefore, the pH-sensing color indicator film can be used as a smart indicator for real-time freshness monitoring of meat and seafood products.
33137387	5	3	theme	color	742:746	arg1	film					758:761	The color indicator film	738:761	The color indicator film	738:761	The color indicator film changed clearly from reddish-pink to pale peach and finally to yellow when exposed to different pH buffers.
33137387	6	4	dep	green	946:950	arg1	pink					933:936	pink	933:936	pink	933:936	However, in response to ammonia vapor, the color changed from pink to pale green and yellow.
33137387	8	5	used	used	1095:1098	arg2	indicator					1111:1119	a smart indicator	1103:1119	a smart indicator for real-time freshness monitoring of meat and seafood products	1103:1183	Therefore, the pH-sensing color indicator film can be used as a smart indicator for real-time freshness monitoring of meat and seafood products.
33137387	8	5	used	used	1095:1098	arg2	film					1083:1086	the pH-sensing color indicator film	1052:1086	the pH-sensing color indicator film	1052:1086	Therefore, the pH-sensing color indicator film can be used as a smart indicator for real-time freshness monitoring of meat and seafood products.
33137387	6	6	theme	pale	941:944	arg1	green					946:950	pale green	941:950	pale green	941:950	However, in response to ammonia vapor, the color changed from pink to pale green and yellow.
33137387	5	7	theme	pH	859:860	arg1	buffers					862:868	different pH buffers	849:868	different pH buffers	849:868	The color indicator film changed clearly from reddish-pink to pale peach and finally to yellow when exposed to different pH buffers.
33137387	1	8	theme	/chitosan	267:275	arg1	film					304:307	/chitosan nanofiber (ChNF) composite film	267:307	methylcellulose (MC)/chitosan nanofiber (ChNF) composite film	247:307	A new pH-responsive color indicator film was prepared by blending barberry anthocyanin (BA) with methylcellulose (MC)/chitosan nanofiber (ChNF) composite film.
33137387	0	9	theme	real-time	110:118	arg1	monitoring					120:129	real-time monitoring	110:129	real-time monitoring of meat freshness	110:147	pH-responsive color indicator films based on methylcellulose/chitosan nanofiber and barberry anthocyanins for real-time monitoring of meat freshness.
33137387	3	10	theme	composite	507:515	arg1	film					517:520	the composite film	503:520	the composite film	503:520	Anthocyanin showed proper compatibility with the composite film.
33137387	1	11	theme	nanofiber	277:285	arg1	film					304:307	/chitosan nanofiber (ChNF) composite film	267:307	methylcellulose (MC)/chitosan nanofiber (ChNF) composite film	247:307	A new pH-responsive color indicator film was prepared by blending barberry anthocyanin (BA) with methylcellulose (MC)/chitosan nanofiber (ChNF) composite film.
33137387	0	12	theme	meat	134:137	arg1	freshness					139:147	meat freshness	134:147	meat freshness	134:147	pH-responsive color indicator films based on methylcellulose/chitosan nanofiber and barberry anthocyanins for real-time monitoring of meat freshness.
33137387	4	13	from	change	573:578	arg1	response					583:590	response	583:590	response to pH changes and ammonia gas	583:620	The color indicator film showed an apparent color change in response to pH changes and ammonia gas, being suitable for indicating the change in food pH, the formation of volatile nitrogen compounds, and food decay.
33137387	4	14	from	change	657:662	arg1	pH					672:673	food pH	667:673	food pH	667:673	The color indicator film showed an apparent color change in response to pH changes and ammonia gas, being suitable for indicating the change in food pH, the formation of volatile nitrogen compounds, and food decay.
33137387	0	15	theme	methylcellulose/chitosan	45:68	arg1	nanofiber					70:78	methylcellulose/chitosan nanofiber	45:78	methylcellulose/chitosan nanofiber	45:78	pH-responsive color indicator films based on methylcellulose/chitosan nanofiber and barberry anthocyanins for real-time monitoring of meat freshness.
33137387	3	16	with	compatibility	484:496	arg1	film					517:520	the composite film	503:520	the composite film	503:520	Anthocyanin showed proper compatibility with the composite film.
33137387	7	17	theme	antioxidant	1019:1029	arg1	activity					1031:1038	remarkable antioxidant activity	1008:1038	remarkable antioxidant activity	1008:1038	Besides, the color indicator film exhibited remarkable antioxidant activity.
33137387	2	18	theme	composite	442:450	arg1	film					452:455	the composite film	438:455	the composite film	438:455	The addition of ChNF and BA increased the mechanical and water barrier properties but reduced the UV-vis light transmittance of the composite film.
33137387	8	19	theme	products	1176:1183	arg1	monitoring					1145:1154	real-time freshness monitoring	1125:1154	real-time freshness monitoring of meat and seafood products	1125:1183	Therefore, the pH-sensing color indicator film can be used as a smart indicator for real-time freshness monitoring of meat and seafood products.
33137387	0	20	theme	color	14:18	arg1	films					30:34	pH-responsive color indicator films	0:34	pH-responsive color indicator films	0:34	pH-responsive color indicator films based on methylcellulose/chitosan nanofiber and barberry anthocyanins for real-time monitoring of meat freshness.
33137387	4	21	theme	color	567:571	arg1	change					573:578	an apparent color change	555:578	an apparent color change in response to pH changes and ammonia gas	555:620	The color indicator film showed an apparent color change in response to pH changes and ammonia gas, being suitable for indicating the change in food pH, the formation of volatile nitrogen compounds, and food decay.
33137387	8	22	theme	meat	1159:1162	arg1	products					1176:1183	meat and seafood products	1159:1183	meat and seafood products	1159:1183	Therefore, the pH-sensing color indicator film can be used as a smart indicator for real-time freshness monitoring of meat and seafood products.
33137387	2	23	theme	barrier	373:379	arg1	properties					381:390	the mechanical and water barrier properties	348:390	properties	381:390	The addition of ChNF and BA increased the mechanical and water barrier properties but reduced the UV-vis light transmittance of the composite film.
33137387	0	24	theme	pH-responsive	0:12	arg1	films					30:34	pH-responsive color indicator films	0:34	pH-responsive color indicator films	0:34	pH-responsive color indicator films based on methylcellulose/chitosan nanofiber and barberry anthocyanins for real-time monitoring of meat freshness.
33137387	4	25	theme	apparent	558:565	arg1	change					573:578	an apparent color change	555:578	an apparent color change in response to pH changes and ammonia gas	555:620	The color indicator film showed an apparent color change in response to pH changes and ammonia gas, being suitable for indicating the change in food pH, the formation of volatile nitrogen compounds, and food decay.
33137387	4	26	theme	volatile	693:700	arg1	compounds					711:719	volatile nitrogen compounds	693:719	volatile nitrogen compounds	693:719	The color indicator film showed an apparent color change in response to pH changes and ammonia gas, being suitable for indicating the change in food pH, the formation of volatile nitrogen compounds, and food decay.
33137387	4	27	theme	compounds	711:719	arg1	change					657:662	the change	653:662	the change in food pH	653:673	The color indicator film showed an apparent color change in response to pH changes and ammonia gas, being suitable for indicating the change in food pH, the formation of volatile nitrogen compounds, and food decay.
33137387	4	27	theme	compounds	711:719	arg1	decay					731:735	food decay	726:735	food decay	726:735	The color indicator film showed an apparent color change in response to pH changes and ammonia gas, being suitable for indicating the change in food pH, the formation of volatile nitrogen compounds, and food decay.
33137387	4	27	theme	compounds	711:719	arg1	formation					680:688	the formation	676:688	the formation of volatile nitrogen compounds	676:719	The color indicator film showed an apparent color change in response to pH changes and ammonia gas, being suitable for indicating the change in food pH, the formation of volatile nitrogen compounds, and food decay.
33137387	2	28	theme	water	367:371	arg1	properties					381:390	the mechanical and water barrier properties	348:390	properties	381:390	The addition of ChNF and BA increased the mechanical and water barrier properties but reduced the UV-vis light transmittance of the composite film.
33137387	1	29	theme	ChNF	288:291	arg1	film					304:307	/chitosan nanofiber (ChNF) composite film	267:307	methylcellulose (MC)/chitosan nanofiber (ChNF) composite film	247:307	A new pH-responsive color indicator film was prepared by blending barberry anthocyanin (BA) with methylcellulose (MC)/chitosan nanofiber (ChNF) composite film.
33137387	7	30	theme	remarkable	1008:1017	arg1	activity					1031:1038	remarkable antioxidant activity	1008:1038	remarkable antioxidant activity	1008:1038	Besides, the color indicator film exhibited remarkable antioxidant activity.
33137387	4	31	from	decay	731:735	arg1	pH					672:673	food pH	667:673	food pH	667:673	The color indicator film showed an apparent color change in response to pH changes and ammonia gas, being suitable for indicating the change in food pH, the formation of volatile nitrogen compounds, and food decay.
33137387	4	32	theme	food	726:729	arg1	decay					731:735	food decay	726:735	food decay	726:735	The color indicator film showed an apparent color change in response to pH changes and ammonia gas, being suitable for indicating the change in food pH, the formation of volatile nitrogen compounds, and food decay.
33137387	1	33	theme	barberry	216:223	arg1	anthocyanin					225:235	barberry anthocyanin	216:235	barberry anthocyanin (BA)	216:240	A new pH-responsive color indicator film was prepared by blending barberry anthocyanin (BA) with methylcellulose (MC)/chitosan nanofiber (ChNF) composite film.
33137387	1	33	theme	barberry	216:223	arg1	BA					238:239	BA	238:239	BA	238:239	A new pH-responsive color indicator film was prepared by blending barberry anthocyanin (BA) with methylcellulose (MC)/chitosan nanofiber (ChNF) composite film.
33137387	0	34	theme	indicator	20:28	arg1	films					30:34	pH-responsive color indicator films	0:34	pH-responsive color indicator films	0:34	pH-responsive color indicator films based on methylcellulose/chitosan nanofiber and barberry anthocyanins for real-time monitoring of meat freshness.
33137387	0	35	theme	freshness	139:147	arg1	monitoring					120:129	real-time monitoring	110:129	real-time monitoring of meat freshness	110:147	pH-responsive color indicator films based on methylcellulose/chitosan nanofiber and barberry anthocyanins for real-time monitoring of meat freshness.
33137387	1	36	dep	methylcellulose	247:261	arg1	film					304:307	/chitosan nanofiber (ChNF) composite film	267:307	methylcellulose (MC)/chitosan nanofiber (ChNF) composite film	247:307	A new pH-responsive color indicator film was prepared by blending barberry anthocyanin (BA) with methylcellulose (MC)/chitosan nanofiber (ChNF) composite film.
33137387	2	37	theme	UV-vis	408:413	arg1	transmittance					421:433	the UV-vis light transmittance	404:433	the UV-vis light transmittance of the composite film	404:455	The addition of ChNF and BA increased the mechanical and water barrier properties but reduced the UV-vis light transmittance of the composite film.
33137387	5	38	theme	indicator	748:756	arg1	film					758:761	The color indicator film	738:761	The color indicator film	738:761	The color indicator film changed clearly from reddish-pink to pale peach and finally to yellow when exposed to different pH buffers.
33137387	1	39	theme	composite	294:302	arg1	film					304:307	/chitosan nanofiber (ChNF) composite film	267:307	methylcellulose (MC)/chitosan nanofiber (ChNF) composite film	247:307	A new pH-responsive color indicator film was prepared by blending barberry anthocyanin (BA) with methylcellulose (MC)/chitosan nanofiber (ChNF) composite film.
33137387	4	40	theme	indicator	533:541	arg1	film					543:546	The color indicator film	523:546	The color indicator film	523:546	The color indicator film showed an apparent color change in response to pH changes and ammonia gas, being suitable for indicating the change in food pH, the formation of volatile nitrogen compounds, and food decay.
33137387	8	41	theme	smart	1105:1109	arg1	film					1083:1086	the pH-sensing color indicator film	1052:1086	the pH-sensing color indicator film	1052:1086	Therefore, the pH-sensing color indicator film can be used as a smart indicator for real-time freshness monitoring of meat and seafood products.
33137387	8	41	theme	smart	1105:1109	arg1	indicator					1111:1119	a smart indicator	1103:1119	a smart indicator for real-time freshness monitoring of meat and seafood products	1103:1183	Therefore, the pH-sensing color indicator film can be used as a smart indicator for real-time freshness monitoring of meat and seafood products.
33137387	8	42	theme	seafood	1168:1174	arg1	products					1176:1183	meat and seafood products	1159:1183	meat and seafood products	1159:1183	Therefore, the pH-sensing color indicator film can be used as a smart indicator for real-time freshness monitoring of meat and seafood products.
33137387	4	43	theme	color	527:531	arg1	film					543:546	The color indicator film	523:546	The color indicator film	523:546	The color indicator film showed an apparent color change in response to pH changes and ammonia gas, being suitable for indicating the change in food pH, the formation of volatile nitrogen compounds, and food decay.
33137387	7	44	theme	color	977:981	arg1	film					993:996	the color indicator film	973:996	the color indicator film	973:996	Besides, the color indicator film exhibited remarkable antioxidant activity.
33137387	6	45	dep	vapor	903:907	arg1	response					883:890	response	883:890	response	883:890	However, in response to ammonia vapor, the color changed from pink to pale green and yellow.
33137387	2	46	theme	BA	335:336	arg1	addition					314:321	The addition	310:321	The addition of ChNF and BA	310:336	The addition of ChNF and BA increased the mechanical and water barrier properties but reduced the UV-vis light transmittance of the composite film.
33137387	2	47	theme	film	452:455	arg1	transmittance					421:433	the UV-vis light transmittance	404:433	the UV-vis light transmittance of the composite film	404:455	The addition of ChNF and BA increased the mechanical and water barrier properties but reduced the UV-vis light transmittance of the composite film.
33137387	6	48	theme	ammonia	895:901	arg1	vapor					903:907	ammonia vapor	895:907	ammonia vapor	895:907	However, in response to ammonia vapor, the color changed from pink to pale green and yellow.
33137387	7	49	theme	indicator	983:991	arg1	film					993:996	the color indicator film	973:996	the color indicator film	973:996	Besides, the color indicator film exhibited remarkable antioxidant activity.
33137387	4	50	theme	nitrogen	702:709	arg1	compounds					711:719	volatile nitrogen compounds	693:719	volatile nitrogen compounds	693:719	The color indicator film showed an apparent color change in response to pH changes and ammonia gas, being suitable for indicating the change in food pH, the formation of volatile nitrogen compounds, and food decay.
33137387	8	51	theme	real-time	1125:1133	arg1	monitoring					1145:1154	real-time freshness monitoring	1125:1154	real-time freshness monitoring of meat and seafood products	1125:1183	Therefore, the pH-sensing color indicator film can be used as a smart indicator for real-time freshness monitoring of meat and seafood products.
33137387	8	52	theme	indicator	1073:1081	arg1	film					1083:1086	the pH-sensing color indicator film	1052:1086	the pH-sensing color indicator film	1052:1086	Therefore, the pH-sensing color indicator film can be used as a smart indicator for real-time freshness monitoring of meat and seafood products.
33137387	8	52	theme	indicator	1073:1081	arg1	indicator					1111:1119	a smart indicator	1103:1119	a smart indicator for real-time freshness monitoring of meat and seafood products	1103:1183	Therefore, the pH-sensing color indicator film can be used as a smart indicator for real-time freshness monitoring of meat and seafood products.
33137387	4	53	theme	food	667:670	arg1	pH					672:673	food pH	667:673	food pH	667:673	The color indicator film showed an apparent color change in response to pH changes and ammonia gas, being suitable for indicating the change in food pH, the formation of volatile nitrogen compounds, and food decay.
33137387	2	54	theme	ChNF	326:329	arg1	addition					314:321	The addition	310:321	The addition of ChNF and BA	310:336	The addition of ChNF and BA increased the mechanical and water barrier properties but reduced the UV-vis light transmittance of the composite film.
33137387	8	55	theme	color	1067:1071	arg1	film					1083:1086	the pH-sensing color indicator film	1052:1086	the pH-sensing color indicator film	1052:1086	Therefore, the pH-sensing color indicator film can be used as a smart indicator for real-time freshness monitoring of meat and seafood products.
33137387	8	55	theme	color	1067:1071	arg1	indicator					1111:1119	a smart indicator	1103:1119	a smart indicator for real-time freshness monitoring of meat and seafood products	1103:1183	Therefore, the pH-sensing color indicator film can be used as a smart indicator for real-time freshness monitoring of meat and seafood products.
33137387	1	56	theme	new	152:154	arg1	film					186:189	A new pH-responsive color indicator film	150:189	A new pH-responsive color indicator film	150:189	A new pH-responsive color indicator film was prepared by blending barberry anthocyanin (BA) with methylcellulose (MC)/chitosan nanofiber (ChNF) composite film.
33137387	3	57	theme	proper	477:482	arg1	compatibility					484:496	proper compatibility	477:496	proper compatibility with the composite film	477:520	Anthocyanin showed proper compatibility with the composite film.
33137387	0	58	theme	barberry	84:91	arg1	anthocyanins					93:104	barberry anthocyanins	84:104	barberry anthocyanins for real-time monitoring of meat freshness	84:147	pH-responsive color indicator films based on methylcellulose/chitosan nanofiber and barberry anthocyanins for real-time monitoring of meat freshness.
33137387	2	59	theme	light	415:419	arg1	transmittance					421:433	the UV-vis light transmittance	404:433	the UV-vis light transmittance of the composite film	404:455	The addition of ChNF and BA increased the mechanical and water barrier properties but reduced the UV-vis light transmittance of the composite film.
33137387	1	60	theme	pH-responsive	156:168	arg1	film					186:189	A new pH-responsive color indicator film	150:189	A new pH-responsive color indicator film	150:189	A new pH-responsive color indicator film was prepared by blending barberry anthocyanin (BA) with methylcellulose (MC)/chitosan nanofiber (ChNF) composite film.
33137387	4	61	from	formation	680:688	arg1	pH					672:673	food pH	667:673	food pH	667:673	The color indicator film showed an apparent color change in response to pH changes and ammonia gas, being suitable for indicating the change in food pH, the formation of volatile nitrogen compounds, and food decay.
33137387	4	62	theme	pH	595:596	arg1	changes					598:604	pH changes	595:604	pH changes	595:604	The color indicator film showed an apparent color change in response to pH changes and ammonia gas, being suitable for indicating the change in food pH, the formation of volatile nitrogen compounds, and food decay.
33137387	8	63	theme	freshness	1135:1143	arg1	monitoring					1145:1154	real-time freshness monitoring	1125:1154	real-time freshness monitoring of meat and seafood products	1125:1183	Therefore, the pH-sensing color indicator film can be used as a smart indicator for real-time freshness monitoring of meat and seafood products.
33137387	1	64	theme	color	170:174	arg1	film					186:189	A new pH-responsive color indicator film	150:189	A new pH-responsive color indicator film	150:189	A new pH-responsive color indicator film was prepared by blending barberry anthocyanin (BA) with methylcellulose (MC)/chitosan nanofiber (ChNF) composite film.
33137387	4	65	theme	ammonia	610:616	arg1	gas					618:620	ammonia gas	610:620	ammonia gas	610:620	The color indicator film showed an apparent color change in response to pH changes and ammonia gas, being suitable for indicating the change in food pH, the formation of volatile nitrogen compounds, and food decay.
32927340	0	0	theme	high-efficiency	101:115	arg1	degradation					127:137	its high-efficiency catalytic degradation	97:137	its high-efficiency catalytic degradation for acridine	97:150	Laccase-mediator system assembling co-immobilized onto functionalized calcium alginate beads and its high-efficiency catalytic degradation for acridine.
32927340	3	1	theme	acridine	799:806	arg1	rate					791:794	the degradation rate	775:794	the degradation rate of acridine	775:806	Treating 30 mg/L acridine aqueous solution for 8 h with the biocatalyst, the degradation rate of acridine reached 100 %.
32927340	6	2	theme	catalytic	1373:1381	arg1	activity					1383:1390	catalytic activity	1373:1390	catalytic activity	1373:1390	The beaded biocatalyst about 3 mm in diameter could be easily recovered and reused several times without obvious deterioration in catalytic activity.
32927340	3	3	theme	degradation	779:789	arg1	rate					791:794	the degradation rate	775:794	the degradation rate of acridine	775:806	Treating 30 mg/L acridine aqueous solution for 8 h with the biocatalyst, the degradation rate of acridine reached 100 %.
32927340	7	4	theme	biocatalytic	1518:1529	arg1	mechanism					1531:1539	the possible biocatalytic mechanism	1505:1539	the possible biocatalytic mechanism	1505:1539	Furthermore, according to the test results of electron paramagnetic resonance and the identified intermediates, the possible biocatalytic mechanism and reaction pathways for acridine degradation have been proposed.
32927340	2	5	theme	laccase-mediator	623:638	arg1	system					640:645	the co-immobilized laccase-mediator system	604:645	the co-immobilized laccase-mediator system	604:645	The enzyme-catalyzed degradation for acridine in water was systematically studied for the first time, and it was found that the co-immobilized laccase-mediator system has excellent capability to degrade acridine in water.
32927340	7	6	theme	paramagnetic	1448:1459	arg1	resonance					1461:1469	electron paramagnetic resonance	1439:1469	electron paramagnetic resonance	1439:1469	Furthermore, according to the test results of electron paramagnetic resonance and the identified intermediates, the possible biocatalytic mechanism and reaction pathways for acridine degradation have been proposed.
32927340	7	7	theme	resonance	1461:1469	arg1	results					1428:1434	the test results	1419:1434	the test results of electron paramagnetic resonance and the identified intermediates	1419:1502	Furthermore, according to the test results of electron paramagnetic resonance and the identified intermediates, the possible biocatalytic mechanism and reaction pathways for acridine degradation have been proposed.
32927340	1	8	theme	functionalized	332:345	arg1	beads					374:378	the functionalized calcium alginate composite beads	328:378	the functionalized calcium alginate composite beads	328:378	A heterogeneous bead-shaped biocatalyst was prepared by assembling co-immobilization of encapsulating mediator 2,2'-Azino-bis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) into the functionalized calcium alginate composite beads by grafting glycidyl methacrylate and dopamine and then immobilizing laccase covalently onto beads.
32927340	0	9	theme	catalytic	117:125	arg1	degradation					127:137	its high-efficiency catalytic degradation	97:137	its high-efficiency catalytic degradation for acridine	97:150	Laccase-mediator system assembling co-immobilized onto functionalized calcium alginate beads and its high-efficiency catalytic degradation for acridine.
32927340	7	10	theme	acridine	1567:1574	arg1	degradation					1576:1586	acridine degradation	1567:1586	acridine degradation	1567:1586	Furthermore, according to the test results of electron paramagnetic resonance and the identified intermediates, the possible biocatalytic mechanism and reaction pathways for acridine degradation have been proposed.
32927340	6	11	theme	obvious	1348:1354	arg1	deterioration					1356:1368	obvious deterioration	1348:1368	obvious deterioration in catalytic activity	1348:1390	The beaded biocatalyst about 3 mm in diameter could be easily recovered and reused several times without obvious deterioration in catalytic activity.
32927340	7	12	theme	reaction	1545:1552	arg1	pathways					1554:1561	reaction pathways	1545:1561	reaction pathways	1545:1561	Furthermore, according to the test results of electron paramagnetic resonance and the identified intermediates, the possible biocatalytic mechanism and reaction pathways for acridine degradation have been proposed.
32927340	6	13	from	biocatalyst	1254:1264	arg1	diameter					1280:1287	diameter	1280:1287	diameter	1280:1287	The beaded biocatalyst about 3 mm in diameter could be easily recovered and reused several times without obvious deterioration in catalytic activity.
32927340	1	14	theme	calcium	347:353	arg1	beads					374:378	the functionalized calcium alginate composite beads	328:378	the functionalized calcium alginate composite beads	328:378	A heterogeneous bead-shaped biocatalyst was prepared by assembling co-immobilization of encapsulating mediator 2,2'-Azino-bis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) into the functionalized calcium alginate composite beads by grafting glycidyl methacrylate and dopamine and then immobilizing laccase covalently onto beads.
32927340	7	15	theme	identified	1479:1488	arg1	intermediates					1490:1502	the identified intermediates	1475:1502	the identified intermediates	1475:1502	Furthermore, according to the test results of electron paramagnetic resonance and the identified intermediates, the possible biocatalytic mechanism and reaction pathways for acridine degradation have been proposed.
32927340	5	16	theme	mediator	1070:1077	arg1	co-immobilization					1033:1049	the effective assembling co-immobilization	1008:1049	the effective assembling co-immobilization of laccase and the mediator so that the catalytic activity of the enzyme and the synergistic promoting action of the mediator have been given full play in the process of acridine degradation	1008:1240	Its outstanding catalytic activity could be attributed to the effective assembling co-immobilization of laccase and the mediator so that the catalytic activity of the enzyme and the synergistic promoting action of the mediator have been given full play in the process of acridine degradation.
32927340	2	17	contain	has	647:649	arg1	system					640:645	the co-immobilized laccase-mediator system	604:645	the co-immobilized laccase-mediator system	604:645	The enzyme-catalyzed degradation for acridine in water was systematically studied for the first time, and it was found that the co-immobilized laccase-mediator system has excellent capability to degrade acridine in water.
32927340	2	17	contain	has	647:649	arg2	capability					661:670	excellent capability	651:670	excellent capability to degrade acridine in water	651:699	The enzyme-catalyzed degradation for acridine in water was systematically studied for the first time, and it was found that the co-immobilized laccase-mediator system has excellent capability to degrade acridine in water.
32927340	2	18	from	acridine	683:690	arg1	water					695:699	water	695:699	water	695:699	The enzyme-catalyzed degradation for acridine in water was systematically studied for the first time, and it was found that the co-immobilized laccase-mediator system has excellent capability to degrade acridine in water.
32927340	4	19	theme	acridine	855:862	arg1	low					920:922	low	920:922	low	920:922	In contrast, the degradation of acridine by free laccase under the same treatment conditions was low and difficult to observe.
32927340	4	19	theme	acridine	855:862	arg1	degradation					840:850	the degradation	836:850	the degradation of acridine by free laccase under the same treatment conditions	836:914	In contrast, the degradation of acridine by free laccase under the same treatment conditions was low and difficult to observe.
32927340	2	20	theme	enzyme-catalyzed	484:499	arg1	degradation					501:511	The enzyme-catalyzed degradation	480:511	The enzyme-catalyzed degradation for acridine in water	480:533	The enzyme-catalyzed degradation for acridine in water was systematically studied for the first time, and it was found that the co-immobilized laccase-mediator system has excellent capability to degrade acridine in water.
32927340	4	21	from	low	920:922	arg1	contrast					826:833	contrast	826:833	contrast	826:833	In contrast, the degradation of acridine by free laccase under the same treatment conditions was low and difficult to observe.
32927340	6	22	theme	several	1326:1332	arg1	times					1334:1338	several times	1326:1338	several times without obvious deterioration in catalytic activity	1326:1390	The beaded biocatalyst about 3 mm in diameter could be easily recovered and reused several times without obvious deterioration in catalytic activity.
32927340	5	23	theme	catalytic	1091:1099	arg1	activity					1101:1108	the catalytic activity	1087:1108	the catalytic activity of the enzyme	1087:1122	Its outstanding catalytic activity could be attributed to the effective assembling co-immobilization of laccase and the mediator so that the catalytic activity of the enzyme and the synergistic promoting action of the mediator have been given full play in the process of acridine degradation.
32927340	5	24	theme	assembling	1022:1031	arg1	co-immobilization					1033:1049	the effective assembling co-immobilization	1008:1049	the effective assembling co-immobilization of laccase and the mediator so that the catalytic activity of the enzyme and the synergistic promoting action of the mediator have been given full play in the process of acridine degradation	1008:1240	Its outstanding catalytic activity could be attributed to the effective assembling co-immobilization of laccase and the mediator so that the catalytic activity of the enzyme and the synergistic promoting action of the mediator have been given full play in the process of acridine degradation.
32927340	0	25	theme	Laccase-mediator	0:15	arg1	system					17:22	Laccase-mediator system	0:22	Laccase-mediator system	0:22	Laccase-mediator system assembling co-immobilized onto functionalized calcium alginate beads and its high-efficiency catalytic degradation for acridine.
32927340	1	26	theme	alginate	355:362	arg1	beads					374:378	the functionalized calcium alginate composite beads	328:378	the functionalized calcium alginate composite beads	328:378	A heterogeneous bead-shaped biocatalyst was prepared by assembling co-immobilization of encapsulating mediator 2,2'-Azino-bis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) into the functionalized calcium alginate composite beads by grafting glycidyl methacrylate and dopamine and then immobilizing laccase covalently onto beads.
32927340	4	27	from	contrast	826:833	arg1	low					920:922	low	920:922	low	920:922	In contrast, the degradation of acridine by free laccase under the same treatment conditions was low and difficult to observe.
32927340	4	27	from	contrast	826:833	arg1	degradation					840:850	the degradation	836:850	the degradation of acridine by free laccase under the same treatment conditions	836:914	In contrast, the degradation of acridine by free laccase under the same treatment conditions was low and difficult to observe.
32927340	7	28	theme	electron	1439:1446	arg1	resonance					1461:1469	electron paramagnetic resonance	1439:1469	electron paramagnetic resonance	1439:1469	Furthermore, according to the test results of electron paramagnetic resonance and the identified intermediates, the possible biocatalytic mechanism and reaction pathways for acridine degradation have been proposed.
32927340	5	29	theme	outstanding	954:964	arg1	activity					976:983	Its outstanding catalytic activity	950:983	Its outstanding catalytic activity	950:983	Its outstanding catalytic activity could be attributed to the effective assembling co-immobilization of laccase and the mediator so that the catalytic activity of the enzyme and the synergistic promoting action of the mediator have been given full play in the process of acridine degradation.
32927340	1	30	theme	composite	364:372	arg1	beads					374:378	the functionalized calcium alginate composite beads	328:378	the functionalized calcium alginate composite beads	328:378	A heterogeneous bead-shaped biocatalyst was prepared by assembling co-immobilization of encapsulating mediator 2,2'-Azino-bis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) into the functionalized calcium alginate composite beads by grafting glycidyl methacrylate and dopamine and then immobilizing laccase covalently onto beads.
32927340	5	31	theme	effective	1012:1020	arg1	co-immobilization					1033:1049	the effective assembling co-immobilization	1008:1049	the effective assembling co-immobilization of laccase and the mediator so that the catalytic activity of the enzyme and the synergistic promoting action of the mediator have been given full play in the process of acridine degradation	1008:1240	Its outstanding catalytic activity could be attributed to the effective assembling co-immobilization of laccase and the mediator so that the catalytic activity of the enzyme and the synergistic promoting action of the mediator have been given full play in the process of acridine degradation.
32927340	7	32	theme	intermediates	1490:1502	arg1	results					1428:1434	the test results	1419:1434	the test results of electron paramagnetic resonance and the identified intermediates	1419:1502	Furthermore, according to the test results of electron paramagnetic resonance and the identified intermediates, the possible biocatalytic mechanism and reaction pathways for acridine degradation have been proposed.
32927340	1	33	theme	mediator	255:262	arg1	ABTS					317:320	ABTS	317:320	ABTS	317:320	A heterogeneous bead-shaped biocatalyst was prepared by assembling co-immobilization of encapsulating mediator 2,2'-Azino-bis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) into the functionalized calcium alginate composite beads by grafting glycidyl methacrylate and dopamine and then immobilizing laccase covalently onto beads.
32927340	1	33	theme	mediator	255:262	arg1	2,2'-Azino-bis-					264:278	mediator 2,2'-Azino-bis-	255:278	mediator 2,2'-Azino-bis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) into the functionalized calcium alginate composite beads	255:378	A heterogeneous bead-shaped biocatalyst was prepared by assembling co-immobilization of encapsulating mediator 2,2'-Azino-bis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) into the functionalized calcium alginate composite beads by grafting glycidyl methacrylate and dopamine and then immobilizing laccase covalently onto beads.
32927340	1	33	theme	mediator	255:262	arg1	3-ethylbenzthiazoline-6-sulphonate					280:313	3-ethylbenzthiazoline-6-sulphonate	280:313	3-ethylbenzthiazoline-6-sulphonate	280:313	A heterogeneous bead-shaped biocatalyst was prepared by assembling co-immobilization of encapsulating mediator 2,2'-Azino-bis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) into the functionalized calcium alginate composite beads by grafting glycidyl methacrylate and dopamine and then immobilizing laccase covalently onto beads.
32927340	7	34	theme	possible	1509:1516	arg1	mechanism					1531:1539	the possible biocatalytic mechanism	1505:1539	the possible biocatalytic mechanism	1505:1539	Furthermore, according to the test results of electron paramagnetic resonance and the identified intermediates, the possible biocatalytic mechanism and reaction pathways for acridine degradation have been proposed.
32927340	2	35	theme	excellent	651:659	arg1	capability					661:670	excellent capability	651:670	excellent capability to degrade acridine in water	651:699	The enzyme-catalyzed degradation for acridine in water was systematically studied for the first time, and it was found that the co-immobilized laccase-mediator system has excellent capability to degrade acridine in water.
32927340	5	36	theme	laccase	1054:1060	arg1	co-immobilization					1033:1049	the effective assembling co-immobilization	1008:1049	the effective assembling co-immobilization of laccase and the mediator so that the catalytic activity of the enzyme and the synergistic promoting action of the mediator have been given full play in the process of acridine degradation	1008:1240	Its outstanding catalytic activity could be attributed to the effective assembling co-immobilization of laccase and the mediator so that the catalytic activity of the enzyme and the synergistic promoting action of the mediator have been given full play in the process of acridine degradation.
32927340	0	37	theme	functionalized	55:68	arg1	beads					87:91	functionalized calcium alginate beads	55:91	functionalized calcium alginate beads	55:91	Laccase-mediator system assembling co-immobilized onto functionalized calcium alginate beads and its high-efficiency catalytic degradation for acridine.
32927340	4	38	theme	treatment	895:903	arg1	conditions					905:914	the same treatment conditions	886:914	the same treatment conditions	886:914	In contrast, the degradation of acridine by free laccase under the same treatment conditions was low and difficult to observe.
32927340	1	39	theme	grafting	383:390	arg1	methacrylate					401:412	grafting glycidyl methacrylate	383:412	grafting glycidyl methacrylate	383:412	A heterogeneous bead-shaped biocatalyst was prepared by assembling co-immobilization of encapsulating mediator 2,2'-Azino-bis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) into the functionalized calcium alginate composite beads by grafting glycidyl methacrylate and dopamine and then immobilizing laccase covalently onto beads.
32927340	5	40	theme	mediator	1168:1175	arg1	activity					1101:1108	the catalytic activity	1087:1108	the catalytic activity of the enzyme	1087:1122	Its outstanding catalytic activity could be attributed to the effective assembling co-immobilization of laccase and the mediator so that the catalytic activity of the enzyme and the synergistic promoting action of the mediator have been given full play in the process of acridine degradation.
32927340	5	40	theme	mediator	1168:1175	arg1	action					1154:1159	the synergistic promoting action	1128:1159	the synergistic promoting action of the mediator	1128:1175	Its outstanding catalytic activity could be attributed to the effective assembling co-immobilization of laccase and the mediator so that the catalytic activity of the enzyme and the synergistic promoting action of the mediator have been given full play in the process of acridine degradation.
32927340	3	41	theme	30	711:712	arg1	mg/L					714:717	mg/L	714:717	mg/L	714:717	Treating 30 mg/L acridine aqueous solution for 8 h with the biocatalyst, the degradation rate of acridine reached 100 %.
32927340	2	42	theme	first	570:574	arg1	time					576:579	the first time	566:579	the first time	566:579	The enzyme-catalyzed degradation for acridine in water was systematically studied for the first time, and it was found that the co-immobilized laccase-mediator system has excellent capability to degrade acridine in water.
32927340	1	43	theme	glycidyl	392:399	arg1	methacrylate					401:412	grafting glycidyl methacrylate	383:412	grafting glycidyl methacrylate	383:412	A heterogeneous bead-shaped biocatalyst was prepared by assembling co-immobilization of encapsulating mediator 2,2'-Azino-bis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) into the functionalized calcium alginate composite beads by grafting glycidyl methacrylate and dopamine and then immobilizing laccase covalently onto beads.
32927340	5	44	theme	enzyme	1117:1122	arg1	activity					1101:1108	the catalytic activity	1087:1108	the catalytic activity of the enzyme	1087:1122	Its outstanding catalytic activity could be attributed to the effective assembling co-immobilization of laccase and the mediator so that the catalytic activity of the enzyme and the synergistic promoting action of the mediator have been given full play in the process of acridine degradation.
32927340	5	44	theme	enzyme	1117:1122	arg1	action					1154:1159	the synergistic promoting action	1128:1159	the synergistic promoting action of the mediator	1128:1175	Its outstanding catalytic activity could be attributed to the effective assembling co-immobilization of laccase and the mediator so that the catalytic activity of the enzyme and the synergistic promoting action of the mediator have been given full play in the process of acridine degradation.
32927340	0	45	theme	calcium	70:76	arg1	beads					87:91	functionalized calcium alginate beads	55:91	functionalized calcium alginate beads	55:91	Laccase-mediator system assembling co-immobilized onto functionalized calcium alginate beads and its high-efficiency catalytic degradation for acridine.
32927340	2	46	from	degradation	501:511	arg1	water					529:533	water	529:533	water	529:533	The enzyme-catalyzed degradation for acridine in water was systematically studied for the first time, and it was found that the co-immobilized laccase-mediator system has excellent capability to degrade acridine in water.
32927340	5	47	theme	acridine	1221:1228	arg1	degradation					1230:1240	acridine degradation	1221:1240	acridine degradation	1221:1240	Its outstanding catalytic activity could be attributed to the effective assembling co-immobilization of laccase and the mediator so that the catalytic activity of the enzyme and the synergistic promoting action of the mediator have been given full play in the process of acridine degradation.
32927340	5	48	theme	synergistic	1132:1142	arg1	action					1154:1159	the synergistic promoting action	1128:1159	the synergistic promoting action of the mediator	1128:1175	Its outstanding catalytic activity could be attributed to the effective assembling co-immobilization of laccase and the mediator so that the catalytic activity of the enzyme and the synergistic promoting action of the mediator have been given full play in the process of acridine degradation.
32927340	3	49	theme	aqueous	728:734	arg1	solution					736:743	30 mg/L acridine aqueous solution	711:743	30 mg/L acridine aqueous solution for 8 h	711:751	Treating 30 mg/L acridine aqueous solution for 8 h with the biocatalyst, the degradation rate of acridine reached 100 %.
32927340	4	50	theme	same	890:893	arg1	conditions					905:914	the same treatment conditions	886:914	the same treatment conditions	886:914	In contrast, the degradation of acridine by free laccase under the same treatment conditions was low and difficult to observe.
32927340	1	51	theme	heterogeneous	155:167	arg1	biocatalyst					181:191	A heterogeneous bead-shaped biocatalyst	153:191	A heterogeneous bead-shaped biocatalyst	153:191	A heterogeneous bead-shaped biocatalyst was prepared by assembling co-immobilization of encapsulating mediator 2,2'-Azino-bis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) into the functionalized calcium alginate composite beads by grafting glycidyl methacrylate and dopamine and then immobilizing laccase covalently onto beads.
32927340	6	52	from	deterioration	1356:1368	arg1	activity					1383:1390	catalytic activity	1373:1390	catalytic activity	1373:1390	The beaded biocatalyst about 3 mm in diameter could be easily recovered and reused several times without obvious deterioration in catalytic activity.
32927340	6	53	theme	beaded	1247:1252	arg1	biocatalyst					1254:1264	The beaded biocatalyst	1243:1264	The beaded biocatalyst about 3 mm in diameter	1243:1287	The beaded biocatalyst about 3 mm in diameter could be easily recovered and reused several times without obvious deterioration in catalytic activity.
32927340	5	54	theme	promoting	1144:1152	arg1	action					1154:1159	the synergistic promoting action	1128:1159	the synergistic promoting action of the mediator	1128:1175	Its outstanding catalytic activity could be attributed to the effective assembling co-immobilization of laccase and the mediator so that the catalytic activity of the enzyme and the synergistic promoting action of the mediator have been given full play in the process of acridine degradation.
32927340	4	55	theme	free	867:870	arg1	laccase					872:878	free laccase	867:878	free laccase	867:878	In contrast, the degradation of acridine by free laccase under the same treatment conditions was low and difficult to observe.
32927340	1	56	theme	bead-shaped	169:179	arg1	biocatalyst					181:191	A heterogeneous bead-shaped biocatalyst	153:191	A heterogeneous bead-shaped biocatalyst	153:191	A heterogeneous bead-shaped biocatalyst was prepared by assembling co-immobilization of encapsulating mediator 2,2'-Azino-bis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) into the functionalized calcium alginate composite beads by grafting glycidyl methacrylate and dopamine and then immobilizing laccase covalently onto beads.
32927340	3	57	theme	mg/L	714:717	arg1	solution					736:743	30 mg/L acridine aqueous solution	711:743	30 mg/L acridine aqueous solution for 8 h	711:751	Treating 30 mg/L acridine aqueous solution for 8 h with the biocatalyst, the degradation rate of acridine reached 100 %.
32927340	5	58	theme	catalytic	966:974	arg1	activity					976:983	Its outstanding catalytic activity	950:983	Its outstanding catalytic activity	950:983	Its outstanding catalytic activity could be attributed to the effective assembling co-immobilization of laccase and the mediator so that the catalytic activity of the enzyme and the synergistic promoting action of the mediator have been given full play in the process of acridine degradation.
32927340	7	59	theme	test	1423:1426	arg1	results					1428:1434	the test results	1419:1434	the test results of electron paramagnetic resonance and the identified intermediates	1419:1502	Furthermore, according to the test results of electron paramagnetic resonance and the identified intermediates, the possible biocatalytic mechanism and reaction pathways for acridine degradation have been proposed.
32927340	0	60	theme	alginate	78:85	arg1	beads					87:91	functionalized calcium alginate beads	55:91	functionalized calcium alginate beads	55:91	Laccase-mediator system assembling co-immobilized onto functionalized calcium alginate beads and its high-efficiency catalytic degradation for acridine.
32927340	5	61	theme	degradation	1230:1240	arg1	process					1210:1216	the process	1206:1216	the process of acridine degradation	1206:1240	Its outstanding catalytic activity could be attributed to the effective assembling co-immobilization of laccase and the mediator so that the catalytic activity of the enzyme and the synergistic promoting action of the mediator have been given full play in the process of acridine degradation.
32927340	3	62	theme	acridine	719:726	arg1	solution					736:743	30 mg/L acridine aqueous solution	711:743	30 mg/L acridine aqueous solution for 8 h	711:751	Treating 30 mg/L acridine aqueous solution for 8 h with the biocatalyst, the degradation rate of acridine reached 100 %.
32927340	2	63	theme	co-immobilized	608:621	arg1	system					640:645	the co-immobilized laccase-mediator system	604:645	the co-immobilized laccase-mediator system	604:645	The enzyme-catalyzed degradation for acridine in water was systematically studied for the first time, and it was found that the co-immobilized laccase-mediator system has excellent capability to degrade acridine in water.
32987069	5	0	theme	molecules	989:997	arg1	behavior					974:981	both monolayer physical and chemical adsorption behavior	926:981	both monolayer physical and chemical adsorption behavior of MB molecules	926:997	Adsorption process followed Pseudo-first-order, Pseudo-second-order kinetic model and Sips isotherm model, owing to both monolayer physical and chemical adsorption behavior of MB molecules onto homogeneous surface of hydrogel.
32987069	4	1	theme	Factor	663:668	arg1	tests					670:674	Factor tests	663:674	Factor tests	663:674	Factor tests indicated that high removal (97%) of MB was achieved via 0.2 g/L hydrogel within 360 min even after 5 adsorption-regeneration cycles.
32987069	1	2	with	adsorbent	219:227	arg1	structure					241:249	stable structure	234:249	stable structure	234:249	MMTNS were introduced into carboxymethyl cellulose-chitosan system to synthesize porous hydrogel adsorbent with stable structure and high dye handling capacity.
32987069	1	2	with	adsorbent	219:227	arg1	capacity					273:280	high dye handling capacity	255:280	high dye handling capacity	255:280	MMTNS were introduced into carboxymethyl cellulose-chitosan system to synthesize porous hydrogel adsorbent with stable structure and high dye handling capacity.
32987069	1	3	theme	adsorbent	219:227	arg1	hydrogel					210:217	porous hydrogel	203:217	porous hydrogel adsorbent with stable structure and high dye handling capacity	203:280	MMTNS were introduced into carboxymethyl cellulose-chitosan system to synthesize porous hydrogel adsorbent with stable structure and high dye handling capacity.
32987069	5	4	theme	MB	986:987	arg1	molecules					989:997	MB molecules	986:997	MB molecules	986:997	Adsorption process followed Pseudo-first-order, Pseudo-second-order kinetic model and Sips isotherm model, owing to both monolayer physical and chemical adsorption behavior of MB molecules onto homogeneous surface of hydrogel.
32987069	6	5	theme	active	1142:1147	arg1	sites					1149:1153	Si active sites	1139:1153	Si active sites	1139:1153	Adsorption mechanism was attributed to ion-exchange, groups combination of carboxyl and hydroxyl, and Si active sites reaction.
32987069	6	5	theme	active	1142:1147	arg1	hydroxyl					1125:1132	carboxyl and hydroxyl	1112:1132	hydroxyl	1125:1132	Adsorption mechanism was attributed to ion-exchange, groups combination of carboxyl and hydroxyl, and Si active sites reaction.
32987069	6	5	theme	active	1142:1147	arg1	carboxyl					1112:1119	carboxyl and hydroxyl	1112:1132	carboxyl	1112:1119	Adsorption mechanism was attributed to ion-exchange, groups combination of carboxyl and hydroxyl, and Si active sites reaction.
32987069	3	6	theme	internal	626:633	arg1	sites					644:648	internal reaction sites	626:648	internal reaction sites in hydrogel	626:660	Morphology characterization revealed that hydrogel possessed microporous open-framework structure, facilitating free entrance of macromolecular MB dye to react with internal reaction sites in hydrogel.
32987069	2	7	from	Al-OH	283:287	arg1	edge					292:295	edge	292:295	edge of MMTNS	292:304	Al-OH on edge of MMTNS formed hydrogen-bond (-OH···+NH3-) with -NH2 on CS, CS then cooperated with CMC via amidation and chains interleaving, forming three-dimensional hydrogel.
32987069	5	8	theme	hydrogel	1027:1034	arg1	surface					1016:1022	homogeneous surface	1004:1022	homogeneous surface of hydrogel	1004:1034	Adsorption process followed Pseudo-first-order, Pseudo-second-order kinetic model and Sips isotherm model, owing to both monolayer physical and chemical adsorption behavior of MB molecules onto homogeneous surface of hydrogel.
32987069	5	9	theme	Adsorption	810:819	arg1	process					821:827	Adsorption process	810:827	Adsorption process	810:827	Adsorption process followed Pseudo-first-order, Pseudo-second-order kinetic model and Sips isotherm model, owing to both monolayer physical and chemical adsorption behavior of MB molecules onto homogeneous surface of hydrogel.
32987069	3	10	theme	free	573:576	arg1	entrance					578:585	free entrance	573:585	free entrance of macromolecular MB dye	573:610	Morphology characterization revealed that hydrogel possessed microporous open-framework structure, facilitating free entrance of macromolecular MB dye to react with internal reaction sites in hydrogel.
32987069	7	11	theme	polysaccharide	1201:1214	arg1	polymers					1216:1223	polysaccharide polymers	1201:1223	polysaccharide polymers	1201:1223	Such hydrogel realized promotion of polysaccharide polymers in materials design and wastewater treatment.
32987069	6	12	theme	groups	1090:1095	arg1	combination					1097:1107	groups combination	1090:1107	groups combination of carboxyl and hydroxyl	1090:1132	Adsorption mechanism was attributed to ion-exchange, groups combination of carboxyl and hydroxyl, and Si active sites reaction.
32987069	5	13	theme	kinetic	878:884	arg1	model					886:890	Pseudo-first-order, Pseudo-second-order kinetic model	838:890	Pseudo-first-order, Pseudo-second-order kinetic model	838:890	Adsorption process followed Pseudo-first-order, Pseudo-second-order kinetic model and Sips isotherm model, owing to both monolayer physical and chemical adsorption behavior of MB molecules onto homogeneous surface of hydrogel.
32987069	2	14	dep	amidation	390:398	arg1	interleaving					411:422	interleaving	411:422	interleaving	411:422	Al-OH on edge of MMTNS formed hydrogen-bond (-OH···+NH3-) with -NH2 on CS, CS then cooperated with CMC via amidation and chains interleaving, forming three-dimensional hydrogel.
32987069	1	15	theme	stable	234:239	arg1	structure					241:249	stable structure	234:249	stable structure	234:249	MMTNS were introduced into carboxymethyl cellulose-chitosan system to synthesize porous hydrogel adsorbent with stable structure and high dye handling capacity.
32987069	5	16	theme	isotherm	901:908	arg1	model					910:914	Sips isotherm model	896:914	Sips isotherm model	896:914	Adsorption process followed Pseudo-first-order, Pseudo-second-order kinetic model and Sips isotherm model, owing to both monolayer physical and chemical adsorption behavior of MB molecules onto homogeneous surface of hydrogel.
32987069	5	17	theme	adsorption	963:972	arg1	behavior					974:981	both monolayer physical and chemical adsorption behavior	926:981	both monolayer physical and chemical adsorption behavior of MB molecules	926:997	Adsorption process followed Pseudo-first-order, Pseudo-second-order kinetic model and Sips isotherm model, owing to both monolayer physical and chemical adsorption behavior of MB molecules onto homogeneous surface of hydrogel.
32987069	7	18	dep	materials	1228:1236	arg1	materials					1228:1236	materials design and wastewater treatment	1228:1268	materials design and wastewater treatment	1228:1268	Such hydrogel realized promotion of polysaccharide polymers in materials design and wastewater treatment.
32987069	7	18	dep	materials	1228:1236	arg1	design					1238:1243	design	1238:1243	design	1238:1243	Such hydrogel realized promotion of polysaccharide polymers in materials design and wastewater treatment.
32987069	7	18	dep	materials	1228:1236	arg1	treatment					1260:1268	wastewater treatment	1249:1268	wastewater treatment	1249:1268	Such hydrogel realized promotion of polysaccharide polymers in materials design and wastewater treatment.
32987069	7	19	from	promotion	1188:1196	arg1	materials					1228:1236	materials design and wastewater treatment	1228:1268	materials design and wastewater treatment	1228:1268	Such hydrogel realized promotion of polysaccharide polymers in materials design and wastewater treatment.
32987069	7	19	from	promotion	1188:1196	arg1	design					1238:1243	design	1238:1243	design	1238:1243	Such hydrogel realized promotion of polysaccharide polymers in materials design and wastewater treatment.
32987069	7	19	from	promotion	1188:1196	arg1	treatment					1260:1268	wastewater treatment	1249:1268	wastewater treatment	1249:1268	Such hydrogel realized promotion of polysaccharide polymers in materials design and wastewater treatment.
32987069	6	20	dep	ion-exchange	1076:1087	arg1	reaction					1155:1162	reaction	1155:1162	reaction	1155:1162	Adsorption mechanism was attributed to ion-exchange, groups combination of carboxyl and hydroxyl, and Si active sites reaction.
32987069	7	21	theme	Such	1165:1168	arg1	hydrogel					1170:1177	Such hydrogel	1165:1177	Such hydrogel	1165:1177	Such hydrogel realized promotion of polysaccharide polymers in materials design and wastewater treatment.
32987069	5	22	theme	chemical	954:961	arg1	behavior					974:981	both monolayer physical and chemical adsorption behavior	926:981	both monolayer physical and chemical adsorption behavior of MB molecules	926:997	Adsorption process followed Pseudo-first-order, Pseudo-second-order kinetic model and Sips isotherm model, owing to both monolayer physical and chemical adsorption behavior of MB molecules onto homogeneous surface of hydrogel.
32987069	6	23	theme	carboxyl	1112:1119	arg1	sites					1149:1153	Si active sites	1139:1153	Si active sites	1139:1153	Adsorption mechanism was attributed to ion-exchange, groups combination of carboxyl and hydroxyl, and Si active sites reaction.
32987069	6	23	theme	carboxyl	1112:1119	arg1	hydroxyl					1125:1132	carboxyl and hydroxyl	1112:1132	hydroxyl	1125:1132	Adsorption mechanism was attributed to ion-exchange, groups combination of carboxyl and hydroxyl, and Si active sites reaction.
32987069	6	23	theme	carboxyl	1112:1119	arg1	carboxyl					1112:1119	carboxyl and hydroxyl	1112:1132	carboxyl	1112:1119	Adsorption mechanism was attributed to ion-exchange, groups combination of carboxyl and hydroxyl, and Si active sites reaction.
32987069	6	23	theme	carboxyl	1112:1119	arg1	ion-exchange					1076:1087	ion-exchange	1076:1087	ion-exchange	1076:1087	Adsorption mechanism was attributed to ion-exchange, groups combination of carboxyl and hydroxyl, and Si active sites reaction.
32987069	6	23	theme	carboxyl	1112:1119	arg1	combination					1097:1107	groups combination	1090:1107	groups combination of carboxyl and hydroxyl	1090:1132	Adsorption mechanism was attributed to ion-exchange, groups combination of carboxyl and hydroxyl, and Si active sites reaction.
32987069	0	24	theme	cellulose-chitosan-montmorillonite	27:60	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of carboxymethyl cellulose-chitosan-montmorillonite	0:60	Synthesis of carboxymethyl cellulose-chitosan-montmorillonite nanosheets composite hydrogel for dye effluent remediation.
32987069	3	25	theme	reaction	635:642	arg1	sites					644:648	internal reaction sites	626:648	internal reaction sites in hydrogel	626:660	Morphology characterization revealed that hydrogel possessed microporous open-framework structure, facilitating free entrance of macromolecular MB dye to react with internal reaction sites in hydrogel.
32987069	3	26	theme	open-framework	534:547	arg1	structure					549:557	microporous open-framework structure	522:557	microporous open-framework structure	522:557	Morphology characterization revealed that hydrogel possessed microporous open-framework structure, facilitating free entrance of macromolecular MB dye to react with internal reaction sites in hydrogel.
32987069	0	27	theme	carboxymethyl	13:25	arg1	cellulose-chitosan-montmorillonite					27:60	carboxymethyl cellulose-chitosan-montmorillonite	13:60	carboxymethyl cellulose-chitosan-montmorillonite	13:60	Synthesis of carboxymethyl cellulose-chitosan-montmorillonite nanosheets composite hydrogel for dye effluent remediation.
32987069	3	28	theme	macromolecular	590:603	arg1	dye					608:610	macromolecular MB dye	590:610	macromolecular MB dye	590:610	Morphology characterization revealed that hydrogel possessed microporous open-framework structure, facilitating free entrance of macromolecular MB dye to react with internal reaction sites in hydrogel.
32987069	1	29	theme	dye	260:262	arg1	capacity					273:280	high dye handling capacity	255:280	high dye handling capacity	255:280	MMTNS were introduced into carboxymethyl cellulose-chitosan system to synthesize porous hydrogel adsorbent with stable structure and high dye handling capacity.
32987069	0	30	theme	composite	73:81	arg1	hydrogel					83:90	composite hydrogel	73:90	composite hydrogel for dye effluent remediation	73:119	Synthesis of carboxymethyl cellulose-chitosan-montmorillonite nanosheets composite hydrogel for dye effluent remediation.
32987069	3	31	contain	possessed	512:520	arg2	structure					549:557	microporous open-framework structure	522:557	microporous open-framework structure	522:557	Morphology characterization revealed that hydrogel possessed microporous open-framework structure, facilitating free entrance of macromolecular MB dye to react with internal reaction sites in hydrogel.
32987069	3	31	contain	possessed	512:520	arg1	hydrogel					503:510	hydrogel	503:510	hydrogel	503:510	Morphology characterization revealed that hydrogel possessed microporous open-framework structure, facilitating free entrance of macromolecular MB dye to react with internal reaction sites in hydrogel.
32987069	1	32	theme	handling	264:271	arg1	capacity					273:280	high dye handling capacity	255:280	high dye handling capacity	255:280	MMTNS were introduced into carboxymethyl cellulose-chitosan system to synthesize porous hydrogel adsorbent with stable structure and high dye handling capacity.
32987069	3	33	theme	Morphology	461:470	arg1	characterization					472:487	Morphology characterization	461:487	Morphology characterization	461:487	Morphology characterization revealed that hydrogel possessed microporous open-framework structure, facilitating free entrance of macromolecular MB dye to react with internal reaction sites in hydrogel.
32987069	5	34	dep	Pseudo-first-order	838:855	arg1	Pseudo-second-order					858:876	Pseudo-second-order	858:876	Pseudo-second-order	858:876	Adsorption process followed Pseudo-first-order, Pseudo-second-order kinetic model and Sips isotherm model, owing to both monolayer physical and chemical adsorption behavior of MB molecules onto homogeneous surface of hydrogel.
32987069	5	35	theme	physical	941:948	arg1	behavior					974:981	both monolayer physical and chemical adsorption behavior	926:981	both monolayer physical and chemical adsorption behavior of MB molecules	926:997	Adsorption process followed Pseudo-first-order, Pseudo-second-order kinetic model and Sips isotherm model, owing to both monolayer physical and chemical adsorption behavior of MB molecules onto homogeneous surface of hydrogel.
32987069	2	36	theme	three-dimensional	433:449	arg1	hydrogel					451:458	three-dimensional hydrogel	433:458	three-dimensional hydrogel	433:458	Al-OH on edge of MMTNS formed hydrogen-bond (-OH···+NH3-) with -NH2 on CS, CS then cooperated with CMC via amidation and chains interleaving, forming three-dimensional hydrogel.
32987069	7	37	theme	wastewater	1249:1258	arg1	materials					1228:1236	materials design and wastewater treatment	1228:1268	materials design and wastewater treatment	1228:1268	Such hydrogel realized promotion of polysaccharide polymers in materials design and wastewater treatment.
32987069	7	37	theme	wastewater	1249:1258	arg1	treatment					1260:1268	wastewater treatment	1249:1268	wastewater treatment	1249:1268	Such hydrogel realized promotion of polysaccharide polymers in materials design and wastewater treatment.
32987069	6	38	theme	Si	1139:1140	arg1	sites					1149:1153	Si active sites	1139:1153	Si active sites	1139:1153	Adsorption mechanism was attributed to ion-exchange, groups combination of carboxyl and hydroxyl, and Si active sites reaction.
32987069	6	38	theme	Si	1139:1140	arg1	hydroxyl					1125:1132	carboxyl and hydroxyl	1112:1132	hydroxyl	1125:1132	Adsorption mechanism was attributed to ion-exchange, groups combination of carboxyl and hydroxyl, and Si active sites reaction.
32987069	6	38	theme	Si	1139:1140	arg1	carboxyl					1112:1119	carboxyl and hydroxyl	1112:1132	carboxyl	1112:1119	Adsorption mechanism was attributed to ion-exchange, groups combination of carboxyl and hydroxyl, and Si active sites reaction.
32987069	5	39	theme	Pseudo-first-order	838:855	arg1	model					886:890	Pseudo-first-order, Pseudo-second-order kinetic model	838:890	Pseudo-first-order, Pseudo-second-order kinetic model	838:890	Adsorption process followed Pseudo-first-order, Pseudo-second-order kinetic model and Sips isotherm model, owing to both monolayer physical and chemical adsorption behavior of MB molecules onto homogeneous surface of hydrogel.
32987069	1	40	theme	carboxymethyl	149:161	arg1	system					182:187	carboxymethyl cellulose-chitosan system to synthesize porous hydrogel adsorbent with stable structure and high dye handling capacity	149:280	carboxymethyl cellulose-chitosan system to synthesize porous hydrogel adsorbent with stable structure and high dye handling capacity	149:280	MMTNS were introduced into carboxymethyl cellulose-chitosan system to synthesize porous hydrogel adsorbent with stable structure and high dye handling capacity.
32987069	5	41	theme	Sips	896:899	arg1	model					910:914	Sips isotherm model	896:914	Sips isotherm model	896:914	Adsorption process followed Pseudo-first-order, Pseudo-second-order kinetic model and Sips isotherm model, owing to both monolayer physical and chemical adsorption behavior of MB molecules onto homogeneous surface of hydrogel.
32987069	6	42	theme	Adsorption	1037:1046	arg1	mechanism					1048:1056	Adsorption mechanism	1037:1056	Adsorption mechanism	1037:1056	Adsorption mechanism was attributed to ion-exchange, groups combination of carboxyl and hydroxyl, and Si active sites reaction.
32987069	7	43	theme	polymers	1216:1223	arg1	promotion					1188:1196	promotion	1188:1196	promotion of polysaccharide polymers in materials design and wastewater treatment	1188:1268	Such hydrogel realized promotion of polysaccharide polymers in materials design and wastewater treatment.
32987069	1	44	theme	cellulose-chitosan	163:180	arg1	system					182:187	carboxymethyl cellulose-chitosan system to synthesize porous hydrogel adsorbent with stable structure and high dye handling capacity	149:280	carboxymethyl cellulose-chitosan system to synthesize porous hydrogel adsorbent with stable structure and high dye handling capacity	149:280	MMTNS were introduced into carboxymethyl cellulose-chitosan system to synthesize porous hydrogel adsorbent with stable structure and high dye handling capacity.
32987069	4	45	theme	0.2 g/L	733:739	arg1	hydrogel					741:748	0.2 g/L hydrogel	733:748	0.2 g/L hydrogel within 360 min	733:763	Factor tests indicated that high removal (97%) of MB was achieved via 0.2 g/L hydrogel within 360 min even after 5 adsorption-regeneration cycles.
32987069	4	46	theme	adsorption-regeneration	778:800	arg1	cycles					802:807	5 adsorption-regeneration cycles	776:807	5 adsorption-regeneration cycles	776:807	Factor tests indicated that high removal (97%) of MB was achieved via 0.2 g/L hydrogel within 360 min even after 5 adsorption-regeneration cycles.
32987069	3	47	from	sites	644:648	arg1	hydrogel					653:660	hydrogel	653:660	hydrogel	653:660	Morphology characterization revealed that hydrogel possessed microporous open-framework structure, facilitating free entrance of macromolecular MB dye to react with internal reaction sites in hydrogel.
32987069	4	48	theme	high	691:694	arg1	removal					696:702	high removal	691:702	high removal (97%) of MB	691:714	Factor tests indicated that high removal (97%) of MB was achieved via 0.2 g/L hydrogel within 360 min even after 5 adsorption-regeneration cycles.
32987069	4	48	theme	high	691:694	arg1	%					707:707	97%	705:707	97%	705:707	Factor tests indicated that high removal (97%) of MB was achieved via 0.2 g/L hydrogel within 360 min even after 5 adsorption-regeneration cycles.
32987069	5	49	theme	homogeneous	1004:1014	arg1	surface					1016:1022	homogeneous surface	1004:1022	homogeneous surface of hydrogel	1004:1034	Adsorption process followed Pseudo-first-order, Pseudo-second-order kinetic model and Sips isotherm model, owing to both monolayer physical and chemical adsorption behavior of MB molecules onto homogeneous surface of hydrogel.
32987069	3	50	theme	dye	608:610	arg1	entrance					578:585	free entrance	573:585	free entrance of macromolecular MB dye	573:610	Morphology characterization revealed that hydrogel possessed microporous open-framework structure, facilitating free entrance of macromolecular MB dye to react with internal reaction sites in hydrogel.
32987069	0	51	theme	effluent	100:107	arg1	remediation					109:119	dye effluent remediation	96:119	dye effluent remediation	96:119	Synthesis of carboxymethyl cellulose-chitosan-montmorillonite nanosheets composite hydrogel for dye effluent remediation.
32987069	3	52	theme	microporous	522:532	arg1	structure					549:557	microporous open-framework structure	522:557	microporous open-framework structure	522:557	Morphology characterization revealed that hydrogel possessed microporous open-framework structure, facilitating free entrance of macromolecular MB dye to react with internal reaction sites in hydrogel.
32987069	2	53	theme	MMTNS	300:304	arg1	edge					292:295	edge	292:295	edge of MMTNS	292:304	Al-OH on edge of MMTNS formed hydrogen-bond (-OH···+NH3-) with -NH2 on CS, CS then cooperated with CMC via amidation and chains interleaving, forming three-dimensional hydrogel.
32987069	1	54	theme	high	255:258	arg1	capacity					273:280	high dye handling capacity	255:280	high dye handling capacity	255:280	MMTNS were introduced into carboxymethyl cellulose-chitosan system to synthesize porous hydrogel adsorbent with stable structure and high dye handling capacity.
32987069	3	55	theme	MB	605:606	arg1	dye					608:610	macromolecular MB dye	590:610	macromolecular MB dye	590:610	Morphology characterization revealed that hydrogel possessed microporous open-framework structure, facilitating free entrance of macromolecular MB dye to react with internal reaction sites in hydrogel.
32987069	4	56	theme	MB	713:714	arg1	removal					696:702	high removal	691:702	high removal (97%) of MB	691:714	Factor tests indicated that high removal (97%) of MB was achieved via 0.2 g/L hydrogel within 360 min even after 5 adsorption-regeneration cycles.
32987069	4	56	theme	MB	713:714	arg1	%					707:707	97%	705:707	97%	705:707	Factor tests indicated that high removal (97%) of MB was achieved via 0.2 g/L hydrogel within 360 min even after 5 adsorption-regeneration cycles.
32987069	6	57	theme	hydroxyl	1125:1132	arg1	sites					1149:1153	Si active sites	1139:1153	Si active sites	1139:1153	Adsorption mechanism was attributed to ion-exchange, groups combination of carboxyl and hydroxyl, and Si active sites reaction.
32987069	6	57	theme	hydroxyl	1125:1132	arg1	hydroxyl					1125:1132	carboxyl and hydroxyl	1112:1132	hydroxyl	1125:1132	Adsorption mechanism was attributed to ion-exchange, groups combination of carboxyl and hydroxyl, and Si active sites reaction.
32987069	6	57	theme	hydroxyl	1125:1132	arg1	carboxyl					1112:1119	carboxyl and hydroxyl	1112:1132	carboxyl	1112:1119	Adsorption mechanism was attributed to ion-exchange, groups combination of carboxyl and hydroxyl, and Si active sites reaction.
32987069	6	57	theme	hydroxyl	1125:1132	arg1	ion-exchange					1076:1087	ion-exchange	1076:1087	ion-exchange	1076:1087	Adsorption mechanism was attributed to ion-exchange, groups combination of carboxyl and hydroxyl, and Si active sites reaction.
32987069	6	57	theme	hydroxyl	1125:1132	arg1	combination					1097:1107	groups combination	1090:1107	groups combination of carboxyl and hydroxyl	1090:1132	Adsorption mechanism was attributed to ion-exchange, groups combination of carboxyl and hydroxyl, and Si active sites reaction.
32987069	0	58	theme	dye	96:98	arg1	remediation					109:119	dye effluent remediation	96:119	dye effluent remediation	96:119	Synthesis of carboxymethyl cellulose-chitosan-montmorillonite nanosheets composite hydrogel for dye effluent remediation.
32987069	1	59	theme	porous	203:208	arg1	hydrogel					210:217	porous hydrogel	203:217	porous hydrogel adsorbent with stable structure and high dye handling capacity	203:280	MMTNS were introduced into carboxymethyl cellulose-chitosan system to synthesize porous hydrogel adsorbent with stable structure and high dye handling capacity.
34432832	2	0	theme	cartilage	463:471	arg1	weakening					450:458	structural weakening	439:458	structural weakening of cartilage	439:471	Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content, both leading to structural weakening of cartilage.
34432832	0	1	from	variable-field	14:27	arg1	cartilage					99:107	articular cartilage	89:107	articular cartilage	89:107	Low-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.
34432832	2	2	from	increase	396:403	arg1	content					414:420	water content	408:420	water content	408:420	Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content, both leading to structural weakening of cartilage.
34432832	1	3	theme	main	137:140	arg1	disease					155:161	the main degenerative disease	133:161	the main degenerative disease of articular cartilage in joints	133:194	Osteoarthritis (OA) as the main degenerative disease of articular cartilage in joints is accompanied by structural and compositional changes in the tissue.
34432832	2	4	from	consequence	284:294	arg1	content					414:420	water content	408:420	water content	408:420	Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content, both leading to structural weakening of cartilage.
34432832	6	5	theme	hydrogen	1014:1021	arg1	components					1023:1032	separate hydrogen components	1005:1032	separate hydrogen components	1005:1032	These measurements allow the quantification of separate hydrogen components and their assignment to defined physical pools which had been discussed repeatedly in the literature, i.e. bulk-like water and a combination of protein hydrogens and strongly bound water.
34432832	0	6	theme	molecular	67:75	arg1	dynamics					77:84	molecular dynamics	67:84	molecular dynamics	67:84	Low-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.
34432832	1	7	theme	degenerative	142:153	arg1	disease					155:161	the main degenerative disease	133:161	the main degenerative disease of articular cartilage in joints	133:194	Osteoarthritis (OA) as the main degenerative disease of articular cartilage in joints is accompanied by structural and compositional changes in the tissue.
34432832	6	8	theme	separate	1005:1012	arg1	components					1023:1032	separate hydrogen components	1005:1032	separate hydrogen components	1005:1032	These measurements allow the quantification of separate hydrogen components and their assignment to defined physical pools which had been discussed repeatedly in the literature, i.e. bulk-like water and a combination of protein hydrogens and strongly bound water.
34432832	0	9	theme	articular	89:97	arg1	cartilage					99:107	articular cartilage	89:107	articular cartilage	89:107	Low-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.
34432832	0	10	from	Low-field	0:8	arg1	cartilage					99:107	articular cartilage	89:107	articular cartilage	89:107	Low-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.
34432832	3	11	theme	cartilage	496:504	arg1	investigations					478:491	NMR investigations	474:491	NMR investigations of cartilage	474:504	NMR investigations of cartilage generally address only the relaxation properties of water.
34432832	7	12	theme	first	1226:1230	arg1	determination					1232:1244	The first determination	1222:1244	The first determination of 2H relaxation dispersion in comparison to 1H dispersion	1222:1303	The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.
34432832	7	13	theme	T1	1448:1449	arg1	dependence					1401:1410	the pronounced magnetic field dependence	1371:1410	the pronounced magnetic field dependence of the longitudinal relaxation time T1	1371:1449	The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.
34432832	3	14	theme	water	558:562	arg1	properties					544:553	only the relaxation properties	524:553	only the relaxation properties of water	524:562	NMR investigations of cartilage generally address only the relaxation properties of water.
34432832	7	15	theme	dispersion	1263:1272	arg1	determination					1232:1244	The first determination	1222:1244	The first determination of 2H relaxation dispersion in comparison to 1H dispersion	1222:1303	The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.
34432832	3	16	theme	relaxation	533:542	arg1	properties					544:553	only the relaxation properties	524:553	only the relaxation properties of water	524:562	NMR investigations of cartilage generally address only the relaxation properties of water.
34432832	6	17	theme	components	1023:1032	arg1	quantification					987:1000	the quantification	983:1000	the quantification of separate hydrogen components and their assignment to defined physical pools which had been discussed repeatedly in the literature, i.e. bulk-like water and a combination of protein hydrogens and strongly bound water	983:1219	These measurements allow the quantification of separate hydrogen components and their assignment to defined physical pools which had been discussed repeatedly in the literature, i.e. bulk-like water and a combination of protein hydrogens and strongly bound water.
34432832	4	18	theme	different	676:684	arg1	stages					686:691	different stages	676:691	different stages	676:691	In this study, two-dimensional (T1-T2) measurements of bovine articular cartilage samples were carried out for different stages of hydration, complemented by molecular exchange with D2O and treatment by trypsin which simulates degeneration by OA.
34432832	4	19	theme	molecular	723:731	arg1	exchange					733:740	molecular exchange	723:740	molecular exchange with D2O and treatment	723:763	In this study, two-dimensional (T1-T2) measurements of bovine articular cartilage samples were carried out for different stages of hydration, complemented by molecular exchange with D2O and treatment by trypsin which simulates degeneration by OA.
34432832	6	20	theme	hydrogens	1186:1194	arg1	combination					1163:1173	a combination	1161:1173	a combination of protein hydrogens	1161:1194	These measurements allow the quantification of separate hydrogen components and their assignment to defined physical pools which had been discussed repeatedly in the literature, i.e. bulk-like water and a combination of protein hydrogens and strongly bound water.
34432832	6	20	theme	hydrogens	1186:1194	arg1	water					1215:1219	strongly bound water	1200:1219	strongly bound water	1200:1219	These measurements allow the quantification of separate hydrogen components and their assignment to defined physical pools which had been discussed repeatedly in the literature, i.e. bulk-like water and a combination of protein hydrogens and strongly bound water.
34432832	6	20	theme	hydrogens	1186:1194	arg1	water					1151:1155	bulk-like water	1141:1155	bulk-like water	1141:1155	These measurements allow the quantification of separate hydrogen components and their assignment to defined physical pools which had been discussed repeatedly in the literature, i.e. bulk-like water and a combination of protein hydrogens and strongly bound water.
34432832	7	21	theme	dominating	1349:1358	arg1	source					1360:1365	the dominating source	1345:1365	the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1	1345:1449	The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.
34432832	7	21	theme	dominating	1349:1358	arg1	interactions					1329:1340	intramolecular interactions	1314:1340	intramolecular interactions	1314:1340	The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.
34432832	4	22	theme	samples	647:653	arg1	measurements					604:615	two-dimensional (T1-T2) measurements	580:615	two-dimensional (T1-T2) measurements of bovine articular cartilage samples	580:653	In this study, two-dimensional (T1-T2) measurements of bovine articular cartilage samples were carried out for different stages of hydration, complemented by molecular exchange with D2O and treatment by trypsin which simulates degeneration by OA.
34432832	5	23	theme	different	893:901	arg1	T2					903:904	very different T2	888:904	very different T2 which suggests liquid-like and solid-like dynamics	888:955	Two signal components were identified in all measurements, characterized by very different T2 which suggests liquid-like and solid-like dynamics.
34432832	4	24	theme	two-dimensional	580:594	arg1	measurements					604:615	two-dimensional (T1-T2) measurements	580:615	two-dimensional (T1-T2) measurements of bovine articular cartilage samples	580:653	In this study, two-dimensional (T1-T2) measurements of bovine articular cartilage samples were carried out for different stages of hydration, complemented by molecular exchange with D2O and treatment by trypsin which simulates degeneration by OA.
34432832	4	25	theme	cartilage	637:645	arg1	samples					647:653	bovine articular cartilage samples	620:653	bovine articular cartilage samples	620:653	In this study, two-dimensional (T1-T2) measurements of bovine articular cartilage samples were carried out for different stages of hydration, complemented by molecular exchange with D2O and treatment by trypsin which simulates degeneration by OA.
34432832	7	26	theme	intramolecular	1314:1327	arg1	source					1360:1365	the dominating source	1345:1365	the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1	1345:1449	The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.
34432832	7	26	theme	intramolecular	1314:1327	arg1	interactions					1329:1340	intramolecular interactions	1314:1340	intramolecular interactions	1314:1340	The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.
34432832	2	27	theme	reduction	301:309	arg1	Degeneration					266:277	Degeneration	266:277	Degeneration	266:277	Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content, both leading to structural weakening of cartilage.
34432832	2	27	theme	reduction	301:309	arg1	consequence					284:294	a consequence	282:294	a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content	282:420	Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content, both leading to structural weakening of cartilage.
34432832	7	28	theme	1H	1291:1292	arg1	dispersion					1294:1303	1H dispersion	1291:1303	1H dispersion	1291:1303	The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.
34432832	1	29	theme	articular	166:174	arg1	cartilage					176:184	articular cartilage	166:184	articular cartilage	166:184	Osteoarthritis (OA) as the main degenerative disease of articular cartilage in joints is accompanied by structural and compositional changes in the tissue.
34432832	5	30	theme	solid-like	937:946	arg1	dynamics					948:955	liquid-like and solid-like dynamics	921:955	liquid-like and solid-like dynamics	921:955	Two signal components were identified in all measurements, characterized by very different T2 which suggests liquid-like and solid-like dynamics.
34432832	1	31	theme	cartilage	176:184	arg1	disease					155:161	the main degenerative disease	133:161	the main degenerative disease of articular cartilage in joints	133:194	Osteoarthritis (OA) as the main degenerative disease of articular cartilage in joints is accompanied by structural and compositional changes in the tissue.
34432832	0	32	theme	NMR	29:31	arg1	studies					44:50	NMR relaxation studies	29:50	NMR relaxation studies	29:50	Low-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.
34432832	7	33	theme	pronounced	1375:1384	arg1	field					1395:1399	the pronounced magnetic field	1371:1399	the pronounced magnetic field dependence of the longitudinal relaxation time T1	1371:1449	The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.
34432832	7	34	theme	magnetic	1386:1393	arg1	field					1395:1399	the pronounced magnetic field	1371:1399	the pronounced magnetic field dependence of the longitudinal relaxation time T1	1371:1449	The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.
34432832	6	35	theme	defined	1058:1064	arg1	pools					1075:1079	defined physical pools	1058:1079	defined physical pools	1058:1079	These measurements allow the quantification of separate hydrogen components and their assignment to defined physical pools which had been discussed repeatedly in the literature, i.e. bulk-like water and a combination of protein hydrogens and strongly bound water.
34432832	7	36	theme	longitudinal	1419:1430	arg1	T1					1448:1449	the longitudinal relaxation time T1	1415:1449	the longitudinal relaxation time T1	1415:1449	The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.
34432832	5	37	theme	signal	816:821	arg1	components					823:832	Two signal components	812:832	Two signal components	812:832	Two signal components were identified in all measurements, characterized by very different T2 which suggests liquid-like and solid-like dynamics.
34432832	2	38	theme	water	408:412	arg1	content					414:420	water content	408:420	water content	408:420	Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content, both leading to structural weakening of cartilage.
34432832	2	39	theme	macromolecules	328:341	arg1	amount					318:323	the amount	314:323	the amount of macromolecules, the so-called proteoglycans	314:370	Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content, both leading to structural weakening of cartilage.
34432832	2	39	theme	macromolecules	328:341	arg1	proteoglycans					358:370	the so-called proteoglycans	344:370	the so-called proteoglycans	344:370	Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content, both leading to structural weakening of cartilage.
34432832	2	39	theme	macromolecules	328:341	arg1	macromolecules					328:341	macromolecules	328:341	macromolecules	328:341	Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content, both leading to structural weakening of cartilage.
34432832	2	40	theme	increase	396:403	arg1	Degeneration					266:277	Degeneration	266:277	Degeneration	266:277	Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content, both leading to structural weakening of cartilage.
34432832	2	40	theme	increase	396:403	arg1	consequence					284:294	a consequence	282:294	a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content	282:420	Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content, both leading to structural weakening of cartilage.
34432832	3	41	theme	NMR	474:476	arg1	investigations					478:491	NMR investigations	474:491	NMR investigations of cartilage	474:504	NMR investigations of cartilage generally address only the relaxation properties of water.
34432832	1	42	from	changes	243:249	arg1	tissue					258:263	the tissue	254:263	the tissue	254:263	Osteoarthritis (OA) as the main degenerative disease of articular cartilage in joints is accompanied by structural and compositional changes in the tissue.
34432832	4	43	theme	articular	627:635	arg1	cartilage					637:645	bovine articular cartilage	620:645	bovine articular cartilage samples	620:653	In this study, two-dimensional (T1-T2) measurements of bovine articular cartilage samples were carried out for different stages of hydration, complemented by molecular exchange with D2O and treatment by trypsin which simulates degeneration by OA.
34432832	0	44	dep	H2O	55:57	arg1	dynamics					77:84	molecular dynamics	67:84	molecular dynamics	67:84	Low-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.
34432832	6	45	theme	bulk-like	1141:1149	arg1	water					1151:1155	bulk-like water	1141:1155	bulk-like water	1141:1155	These measurements allow the quantification of separate hydrogen components and their assignment to defined physical pools which had been discussed repeatedly in the literature, i.e. bulk-like water and a combination of protein hydrogens and strongly bound water.
34432832	2	46	theme	corresponding	382:394	arg1	increase					396:403	a corresponding increase	380:403	a corresponding increase in water content	380:420	Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content, both leading to structural weakening of cartilage.
34432832	0	47	theme	relaxation	33:42	arg1	studies					44:50	NMR relaxation studies	29:50	NMR relaxation studies	29:50	Low-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.
34432832	2	48	theme	structural	439:448	arg1	weakening					450:458	structural weakening	439:458	structural weakening of cartilage	439:471	Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content, both leading to structural weakening of cartilage.
34432832	7	49	theme	relaxation	1432:1441	arg1	T1					1448:1449	the longitudinal relaxation time T1	1415:1449	the longitudinal relaxation time T1	1415:1449	The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.
34432832	6	50	theme	physical	1066:1073	arg1	pools					1075:1079	defined physical pools	1058:1079	defined physical pools	1058:1079	These measurements allow the quantification of separate hydrogen components and their assignment to defined physical pools which had been discussed repeatedly in the literature, i.e. bulk-like water and a combination of protein hydrogens and strongly bound water.
34432832	4	51	theme	bovine	620:625	arg1	cartilage					637:645	bovine articular cartilage	620:645	bovine articular cartilage samples	620:653	In this study, two-dimensional (T1-T2) measurements of bovine articular cartilage samples were carried out for different stages of hydration, complemented by molecular exchange with D2O and treatment by trypsin which simulates degeneration by OA.
34432832	0	52	theme	H2O	55:57	arg1	variable-field					14:27	variable-field	14:27	variable-field	14:27	Low-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.
34432832	0	52	theme	H2O	55:57	arg1	Low-field					0:8	Low-field	0:8	Low-field	0:8	Low-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.
34432832	4	53	with	exchange	733:740	arg1	treatment					755:763	treatment	755:763	treatment	755:763	In this study, two-dimensional (T1-T2) measurements of bovine articular cartilage samples were carried out for different stages of hydration, complemented by molecular exchange with D2O and treatment by trypsin which simulates degeneration by OA.
34432832	4	53	with	exchange	733:740	arg1	D2O					747:749	D2O	747:749	D2O	747:749	In this study, two-dimensional (T1-T2) measurements of bovine articular cartilage samples were carried out for different stages of hydration, complemented by molecular exchange with D2O and treatment by trypsin which simulates degeneration by OA.
34432832	4	54	theme	T1-T2	597:601	arg1	measurements					604:615	two-dimensional (T1-T2) measurements	580:615	two-dimensional (T1-T2) measurements of bovine articular cartilage samples	580:653	In this study, two-dimensional (T1-T2) measurements of bovine articular cartilage samples were carried out for different stages of hydration, complemented by molecular exchange with D2O and treatment by trypsin which simulates degeneration by OA.
34432832	4	55	theme	hydration	696:704	arg1	stages					686:691	different stages	676:691	different stages	676:691	In this study, two-dimensional (T1-T2) measurements of bovine articular cartilage samples were carried out for different stages of hydration, complemented by molecular exchange with D2O and treatment by trypsin which simulates degeneration by OA.
34432832	2	56	theme	amount	318:323	arg1	reduction					301:309	a reduction	299:309	a reduction of the amount of macromolecules, the so-called proteoglycans	299:370	Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content, both leading to structural weakening of cartilage.
34432832	6	57	theme	bound	1209:1213	arg1	water					1215:1219	strongly bound water	1200:1219	strongly bound water	1200:1219	These measurements allow the quantification of separate hydrogen components and their assignment to defined physical pools which had been discussed repeatedly in the literature, i.e. bulk-like water and a combination of protein hydrogens and strongly bound water.
34432832	7	58	from	determination	1232:1244	arg1	comparison					1277:1286	comparison	1277:1286	comparison to 1H dispersion	1277:1303	The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.
34432832	7	59	theme	field	1395:1399	arg1	dependence					1401:1410	the pronounced magnetic field dependence	1371:1410	the pronounced magnetic field dependence of the longitudinal relaxation time T1	1371:1449	The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.
34432832	7	60	from	dispersion	1263:1272	arg1	comparison					1277:1286	comparison	1277:1286	comparison to 1H dispersion	1277:1303	The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.
34432832	7	61	theme	time	1443:1446	arg1	T1					1448:1449	the longitudinal relaxation time T1	1415:1449	the longitudinal relaxation time T1	1415:1449	The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.
34432832	6	62	theme	assignment	1044:1053	arg1	quantification					987:1000	the quantification	983:1000	the quantification of separate hydrogen components and their assignment to defined physical pools which had been discussed repeatedly in the literature, i.e. bulk-like water and a combination of protein hydrogens and strongly bound water	983:1219	These measurements allow the quantification of separate hydrogen components and their assignment to defined physical pools which had been discussed repeatedly in the literature, i.e. bulk-like water and a combination of protein hydrogens and strongly bound water.
34432832	6	63	theme	protein	1178:1184	arg1	hydrogens					1186:1194	protein hydrogens	1178:1194	protein hydrogens	1178:1194	These measurements allow the quantification of separate hydrogen components and their assignment to defined physical pools which had been discussed repeatedly in the literature, i.e. bulk-like water and a combination of protein hydrogens and strongly bound water.
34432832	5	64	dep	identified	839:848	arg1	characterized					871:883	characterized	871:883	characterized by very different T2 which suggests liquid-like and solid-like dynamics	871:955	Two signal components were identified in all measurements, characterized by very different T2 which suggests liquid-like and solid-like dynamics.
34432832	5	65	theme	liquid-like	921:931	arg1	dynamics					948:955	liquid-like and solid-like dynamics	921:955	liquid-like and solid-like dynamics	921:955	Two signal components were identified in all measurements, characterized by very different T2 which suggests liquid-like and solid-like dynamics.
34432832	1	66	theme	structural	214:223	arg1	changes					243:249	structural and compositional changes	214:249	structural and compositional changes in the tissue	214:263	Osteoarthritis (OA) as the main degenerative disease of articular cartilage in joints is accompanied by structural and compositional changes in the tissue.
34432832	0	67	theme	D2O	63:65	arg1	variable-field					14:27	variable-field	14:27	variable-field	14:27	Low-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.
34432832	0	67	theme	D2O	63:65	arg1	Low-field					0:8	Low-field	0:8	Low-field	0:8	Low-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.
34432832	6	68	dep	water	1151:1155	arg1	i.e.					1136:1139	i.e.	1136:1139	i.e.	1136:1139	These measurements allow the quantification of separate hydrogen components and their assignment to defined physical pools which had been discussed repeatedly in the literature, i.e. bulk-like water and a combination of protein hydrogens and strongly bound water.
34432832	2	69	theme	so-called	348:356	arg1	proteoglycans					358:370	the so-called proteoglycans	344:370	the so-called proteoglycans	344:370	Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content, both leading to structural weakening of cartilage.
34432832	2	69	theme	so-called	348:356	arg1	macromolecules					328:341	macromolecules	328:341	macromolecules	328:341	Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content, both leading to structural weakening of cartilage.
34432832	7	70	from	comparison	1277:1286	arg1	determination					1232:1244	The first determination	1222:1244	The first determination of 2H relaxation dispersion in comparison to 1H dispersion	1222:1303	The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.
34432832	7	71	theme	2H	1249:1250	arg1	dispersion					1263:1272	2H relaxation dispersion	1249:1272	2H relaxation dispersion in comparison to 1H dispersion	1249:1303	The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.
34432832	2	72	from	content	414:420	arg1	Degeneration					266:277	Degeneration	266:277	Degeneration	266:277	Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content, both leading to structural weakening of cartilage.
34432832	2	72	from	content	414:420	arg1	consequence					284:294	a consequence	282:294	a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content	282:420	Degeneration is a consequence of a reduction of the amount of macromolecules, the so-called proteoglycans, and of a corresponding increase in water content, both leading to structural weakening of cartilage.
34432832	0	73	dep	Low-field	0:8	arg1	studies					44:50	NMR relaxation studies	29:50	NMR relaxation studies	29:50	Low-field and variable-field NMR relaxation studies of H2O and D2O molecular dynamics in articular cartilage.
34432832	1	74	from	disease	155:161	arg1	joints					189:194	joints	189:194	joints	189:194	Osteoarthritis (OA) as the main degenerative disease of articular cartilage in joints is accompanied by structural and compositional changes in the tissue.
34432832	7	75	theme	relaxation	1252:1261	arg1	dispersion					1263:1272	2H relaxation dispersion	1249:1272	2H relaxation dispersion in comparison to 1H dispersion	1249:1303	The first determination of 2H relaxation dispersion in comparison to 1H dispersion suggests intramolecular interactions as the dominating source for the pronounced magnetic field dependence of the longitudinal relaxation time T1.
34432832	1	76	theme	compositional	229:241	arg1	changes					243:249	structural and compositional changes	214:249	structural and compositional changes in the tissue	214:263	Osteoarthritis (OA) as the main degenerative disease of articular cartilage in joints is accompanied by structural and compositional changes in the tissue.
32854073	9	0	theme	component	1490:1498	arg1	relation					1464:1471	the distinct structure-mechanics relation	1431:1471	the distinct structure-mechanics relation of each hydrogel component to eventually mimic the response of native tissues	1431:1549	Our systematic approach highlights the importance of single component, multi-modal mechanical analysis of hydrogels to understand the distinct structure-mechanics relation of each hydrogel component to eventually mimic the response of native tissues.
32854073	10	1	theme	engineering	1678:1688	arg1	applications					1690:1701	tissue engineering applications	1671:1701	tissue engineering applications	1671:1701	The presented dataset will allow for the structurally derived compositional design of hydrogels for a broad variety of tissue engineering applications.
32854073	2	2	dep	complex	298:304	arg1	the					294:296	the	294:296	the	294:296	However, the successful creation of functional tissue equivalents requires the comprehensive understanding of the complex and nonlinear mechanical properties of both native tissues and biomaterials.
32854073	1	3	theme	key	151:153	arg1	route					155:159	one key route	147:159	one key route in tissue engineering	147:181	Mimicking the mechanical properties of native human tissues is one key route in tissue engineering.
32854073	9	4	theme	tissues	1543:1549	arg1	response					1524:1531	the response	1520:1531	the response of native tissues	1520:1549	Our systematic approach highlights the importance of single component, multi-modal mechanical analysis of hydrogels to understand the distinct structure-mechanics relation of each hydrogel component to eventually mimic the response of native tissues.
32854073	5	5	theme	brain	956:960	arg1	tissue					962:967	brain tissue	956:967	brain tissue	956:967	Blended together, alginate-gelatin (ALG-GEL) hydrogels can resemble the characteristic nonlinear, conditioning, and compression-tension-asymmetric behavior of brain tissue.
32854073	9	6	theme	hydrogels	1407:1415	arg1	analysis					1395:1402	multi-modal mechanical analysis	1372:1402	multi-modal mechanical analysis of hydrogels	1372:1415	Our systematic approach highlights the importance of single component, multi-modal mechanical analysis of hydrogels to understand the distinct structure-mechanics relation of each hydrogel component to eventually mimic the response of native tissues.
32854073	5	7	theme	compression-tension-asymmetric	913:942	arg1	behavior					944:951	the characteristic nonlinear, conditioning, and compression-tension-asymmetric behavior	865:951	the characteristic nonlinear, conditioning, and compression-tension-asymmetric behavior of brain tissue	865:967	Blended together, alginate-gelatin (ALG-GEL) hydrogels can resemble the characteristic nonlinear, conditioning, and compression-tension-asymmetric behavior of brain tissue.
32854073	3	8	theme	brain	514:518	arg1	tissue					520:525	porcine brain tissue	506:525	porcine brain tissue	506:525	Here, we demonstrate that it is possible to replicate the complex mechanical behavior of soft tissues, exemplary shown for porcine brain tissue, under multiple loading conditions, compression, tension, and torsional shear, through simple blends of alginate and gelatin hydrogels.
32854073	6	9	theme	stiffness	1049:1057	arg1	behavior					1103:1110	the stiffness and loading-mode-specific stress relaxation behavior	1045:1110	the stiffness and loading-mode-specific stress relaxation behavior	1045:1110	We demonstrate that hydrogel concentration and incubation effectively tune the stiffness and loading-mode-specific stress relaxation behavior.
32854073	8	10	theme	stress	1254:1259	arg1	relaxation					1261:1270	slower stress relaxation	1247:1270	slower stress relaxation	1247:1270	In addition, we observe slower stress relaxation after long incubation times.
32854073	7	11	theme	incubation	1206:1215	arg1	time					1217:1220	incubation time	1206:1220	incubation time	1206:1220	The stiffness increases with increasing hydrogel concentration and decreases with increasing incubation time.
32854073	1	12	from	route	155:159	arg1	engineering					171:181	tissue engineering	164:181	tissue engineering	164:181	Mimicking the mechanical properties of native human tissues is one key route in tissue engineering.
32854073	2	13	theme	complex	298:304	arg1	understanding					277:289	the comprehensive understanding	259:289	the comprehensive understanding of the complex and nonlinear mechanical properties of both native tissues and biomaterials	259:380	However, the successful creation of functional tissue equivalents requires the comprehensive understanding of the complex and nonlinear mechanical properties of both native tissues and biomaterials.
32854073	10	14	link	derived	1606:1612	arg1	design					1628:1633	the structurally derived compositional design	1589:1633	the structurally derived compositional design of hydrogels	1589:1646	The presented dataset will allow for the structurally derived compositional design of hydrogels for a broad variety of tissue engineering applications.
32854073	8	15	theme	slower	1247:1252	arg1	relaxation					1261:1270	slower stress relaxation	1247:1270	slower stress relaxation	1247:1270	In addition, we observe slower stress relaxation after long incubation times.
32854073	2	16	theme	biomaterials	369:380	arg1	complex					298:304	complex	298:304	complex	298:304	However, the successful creation of functional tissue equivalents requires the comprehensive understanding of the complex and nonlinear mechanical properties of both native tissues and biomaterials.
32854073	2	16	theme	biomaterials	369:380	arg1	properties					331:340	nonlinear mechanical properties	310:340	nonlinear mechanical properties	310:340	However, the successful creation of functional tissue equivalents requires the comprehensive understanding of the complex and nonlinear mechanical properties of both native tissues and biomaterials.
32854073	4	17	theme	nonlinear	730:738	arg1	behavior					740:747	a nonlinear behavior	728:747	a nonlinear behavior	728:747	Alginate exhibits a pronounced compression-tension asymmetry and a nonlinear behavior, while gelatin shows an almost linear response.
32854073	10	18	theme	compositional	1614:1626	arg1	design					1628:1633	the structurally derived compositional design	1589:1633	the structurally derived compositional design of hydrogels	1589:1646	The presented dataset will allow for the structurally derived compositional design of hydrogels for a broad variety of tissue engineering applications.
32854073	5	19	theme	nonlinear	884:892	arg1	behavior					944:951	the characteristic nonlinear, conditioning, and compression-tension-asymmetric behavior	865:951	the characteristic nonlinear, conditioning, and compression-tension-asymmetric behavior of brain tissue	865:967	Blended together, alginate-gelatin (ALG-GEL) hydrogels can resemble the characteristic nonlinear, conditioning, and compression-tension-asymmetric behavior of brain tissue.
32854073	5	20	theme	tissue	962:967	arg1	behavior					944:951	the characteristic nonlinear, conditioning, and compression-tension-asymmetric behavior	865:951	the characteristic nonlinear, conditioning, and compression-tension-asymmetric behavior of brain tissue	865:967	Blended together, alginate-gelatin (ALG-GEL) hydrogels can resemble the characteristic nonlinear, conditioning, and compression-tension-asymmetric behavior of brain tissue.
32854073	3	21	theme	complex	441:447	arg1	behavior					460:467	the complex mechanical behavior	437:467	the complex mechanical behavior of soft tissues, exemplary shown for porcine brain tissue	437:525	Here, we demonstrate that it is possible to replicate the complex mechanical behavior of soft tissues, exemplary shown for porcine brain tissue, under multiple loading conditions, compression, tension, and torsional shear, through simple blends of alginate and gelatin hydrogels.
32854073	9	22	theme	distinct	1435:1442	arg1	relation					1464:1471	the distinct structure-mechanics relation	1431:1471	the distinct structure-mechanics relation of each hydrogel component to eventually mimic the response of native tissues	1431:1549	Our systematic approach highlights the importance of single component, multi-modal mechanical analysis of hydrogels to understand the distinct structure-mechanics relation of each hydrogel component to eventually mimic the response of native tissues.
32854073	10	23	theme	derived	1606:1612	arg1	design					1628:1633	the structurally derived compositional design	1589:1633	the structurally derived compositional design of hydrogels	1589:1646	The presented dataset will allow for the structurally derived compositional design of hydrogels for a broad variety of tissue engineering applications.
32854073	10	24	theme	presented	1556:1564	arg1	dataset					1566:1572	The presented dataset	1552:1572	The presented dataset	1552:1572	The presented dataset will allow for the structurally derived compositional design of hydrogels for a broad variety of tissue engineering applications.
32854073	3	25	theme	multiple	534:541	arg1	conditions					551:560	multiple loading conditions	534:560	multiple loading conditions	534:560	Here, we demonstrate that it is possible to replicate the complex mechanical behavior of soft tissues, exemplary shown for porcine brain tissue, under multiple loading conditions, compression, tension, and torsional shear, through simple blends of alginate and gelatin hydrogels.
32854073	1	26	theme	tissue	164:169	arg1	engineering					171:181	tissue engineering	164:181	tissue engineering	164:181	Mimicking the mechanical properties of native human tissues is one key route in tissue engineering.
32854073	5	27	theme	alginate-gelatin	815:830	arg1	hydrogels					842:850	alginate-gelatin (ALG-GEL) hydrogels	815:850	alginate-gelatin (ALG-GEL) hydrogels	815:850	Blended together, alginate-gelatin (ALG-GEL) hydrogels can resemble the characteristic nonlinear, conditioning, and compression-tension-asymmetric behavior of brain tissue.
32854073	8	28	theme	long	1278:1281	arg1	times					1294:1298	long incubation times	1278:1298	long incubation times	1278:1298	In addition, we observe slower stress relaxation after long incubation times.
32854073	9	29	theme	systematic	1305:1314	arg1	approach					1316:1323	Our systematic approach	1301:1323	Our systematic approach	1301:1323	Our systematic approach highlights the importance of single component, multi-modal mechanical analysis of hydrogels to understand the distinct structure-mechanics relation of each hydrogel component to eventually mimic the response of native tissues.
32854073	2	30	theme	properties	331:340	arg1	understanding					277:289	the comprehensive understanding	259:289	the comprehensive understanding of the complex and nonlinear mechanical properties of both native tissues and biomaterials	259:380	However, the successful creation of functional tissue equivalents requires the comprehensive understanding of the complex and nonlinear mechanical properties of both native tissues and biomaterials.
32854073	0	31	theme	Alginate-based	0:13	arg1	hydrogels					15:23	Alginate-based hydrogels	0:23	Alginate-based hydrogels	0:23	Alginate-based hydrogels show the same complex mechanical behavior as brain tissue.
32854073	9	32	theme	mechanical	1384:1393	arg1	analysis					1395:1402	multi-modal mechanical analysis	1372:1402	multi-modal mechanical analysis of hydrogels	1372:1415	Our systematic approach highlights the importance of single component, multi-modal mechanical analysis of hydrogels to understand the distinct structure-mechanics relation of each hydrogel component to eventually mimic the response of native tissues.
32854073	5	33	theme	characteristic	869:882	arg1	behavior					944:951	the characteristic nonlinear, conditioning, and compression-tension-asymmetric behavior	865:951	the characteristic nonlinear, conditioning, and compression-tension-asymmetric behavior of brain tissue	865:967	Blended together, alginate-gelatin (ALG-GEL) hydrogels can resemble the characteristic nonlinear, conditioning, and compression-tension-asymmetric behavior of brain tissue.
32854073	2	34	theme	mechanical	320:329	arg1	properties					331:340	nonlinear mechanical properties	310:340	nonlinear mechanical properties	310:340	However, the successful creation of functional tissue equivalents requires the comprehensive understanding of the complex and nonlinear mechanical properties of both native tissues and biomaterials.
32854073	5	35	theme	conditioning	895:906	arg1	behavior					944:951	the characteristic nonlinear, conditioning, and compression-tension-asymmetric behavior	865:951	the characteristic nonlinear, conditioning, and compression-tension-asymmetric behavior of brain tissue	865:967	Blended together, alginate-gelatin (ALG-GEL) hydrogels can resemble the characteristic nonlinear, conditioning, and compression-tension-asymmetric behavior of brain tissue.
32854073	7	36	theme	hydrogel	1153:1160	arg1	concentration					1162:1174	hydrogel concentration	1153:1174	hydrogel concentration	1153:1174	The stiffness increases with increasing hydrogel concentration and decreases with increasing incubation time.
32854073	2	37	theme	nonlinear	310:318	arg1	properties					331:340	nonlinear mechanical properties	310:340	nonlinear mechanical properties	310:340	However, the successful creation of functional tissue equivalents requires the comprehensive understanding of the complex and nonlinear mechanical properties of both native tissues and biomaterials.
32854073	3	38	theme	torsional	589:597	arg1	shear					599:603	torsional shear	589:603	torsional shear	589:603	Here, we demonstrate that it is possible to replicate the complex mechanical behavior of soft tissues, exemplary shown for porcine brain tissue, under multiple loading conditions, compression, tension, and torsional shear, through simple blends of alginate and gelatin hydrogels.
32854073	3	39	theme	soft	472:475	arg1	exemplary					486:494	exemplary	486:494	exemplary	486:494	Here, we demonstrate that it is possible to replicate the complex mechanical behavior of soft tissues, exemplary shown for porcine brain tissue, under multiple loading conditions, compression, tension, and torsional shear, through simple blends of alginate and gelatin hydrogels.
32854073	3	39	theme	soft	472:475	arg1	tissues					477:483	soft tissues	472:483	soft tissues	472:483	Here, we demonstrate that it is possible to replicate the complex mechanical behavior of soft tissues, exemplary shown for porcine brain tissue, under multiple loading conditions, compression, tension, and torsional shear, through simple blends of alginate and gelatin hydrogels.
32854073	9	40	theme	hydrogel	1481:1488	arg1	component					1490:1498	each hydrogel component	1476:1498	each hydrogel component	1476:1498	Our systematic approach highlights the importance of single component, multi-modal mechanical analysis of hydrogels to understand the distinct structure-mechanics relation of each hydrogel component to eventually mimic the response of native tissues.
32854073	10	41	theme	hydrogels	1638:1646	arg1	design					1628:1633	the structurally derived compositional design	1589:1633	the structurally derived compositional design of hydrogels	1589:1646	The presented dataset will allow for the structurally derived compositional design of hydrogels for a broad variety of tissue engineering applications.
32854073	9	42	theme	single	1354:1359	arg1	component					1361:1369	single component	1354:1369	single component	1354:1369	Our systematic approach highlights the importance of single component, multi-modal mechanical analysis of hydrogels to understand the distinct structure-mechanics relation of each hydrogel component to eventually mimic the response of native tissues.
32854073	4	43	theme	compression-tension	694:712	arg1	asymmetry					714:722	a pronounced compression-tension asymmetry	681:722	a pronounced compression-tension asymmetry	681:722	Alginate exhibits a pronounced compression-tension asymmetry and a nonlinear behavior, while gelatin shows an almost linear response.
32854073	3	44	theme	tissues	477:483	arg1	behavior					460:467	the complex mechanical behavior	437:467	the complex mechanical behavior of soft tissues, exemplary shown for porcine brain tissue	437:525	Here, we demonstrate that it is possible to replicate the complex mechanical behavior of soft tissues, exemplary shown for porcine brain tissue, under multiple loading conditions, compression, tension, and torsional shear, through simple blends of alginate and gelatin hydrogels.
32854073	0	45	theme	complex	39:45	arg1	behavior					58:65	the same complex mechanical behavior	30:65	the same complex mechanical behavior as brain tissue	30:81	Alginate-based hydrogels show the same complex mechanical behavior as brain tissue.
32854073	2	46	theme	native	350:355	arg1	tissues					357:363	native tissues	350:363	native tissues	350:363	However, the successful creation of functional tissue equivalents requires the comprehensive understanding of the complex and nonlinear mechanical properties of both native tissues and biomaterials.
32854073	3	47	theme	gelatin	644:650	arg1	blends					621:626	simple blends	614:626	simple blends of alginate and gelatin hydrogels	614:660	Here, we demonstrate that it is possible to replicate the complex mechanical behavior of soft tissues, exemplary shown for porcine brain tissue, under multiple loading conditions, compression, tension, and torsional shear, through simple blends of alginate and gelatin hydrogels.
32854073	2	48	theme	tissue	231:236	arg1	equivalents					238:248	functional tissue equivalents	220:248	functional tissue equivalents	220:248	However, the successful creation of functional tissue equivalents requires the comprehensive understanding of the complex and nonlinear mechanical properties of both native tissues and biomaterials.
32854073	0	49	theme	same	34:37	arg1	behavior					58:65	the same complex mechanical behavior	30:65	the same complex mechanical behavior as brain tissue	30:81	Alginate-based hydrogels show the same complex mechanical behavior as brain tissue.
32854073	6	50	theme	hydrogel	990:997	arg1	concentration					999:1011	hydrogel concentration	990:1011	hydrogel concentration	990:1011	We demonstrate that hydrogel concentration and incubation effectively tune the stiffness and loading-mode-specific stress relaxation behavior.
32854073	2	51	theme	functional	220:229	arg1	equivalents					238:248	functional tissue equivalents	220:248	functional tissue equivalents	220:248	However, the successful creation of functional tissue equivalents requires the comprehensive understanding of the complex and nonlinear mechanical properties of both native tissues and biomaterials.
32854073	10	52	theme	tissue	1671:1676	arg1	applications					1690:1701	tissue engineering applications	1671:1701	tissue engineering applications	1671:1701	The presented dataset will allow for the structurally derived compositional design of hydrogels for a broad variety of tissue engineering applications.
32854073	3	53	theme	porcine	506:512	arg1	tissue					520:525	porcine brain tissue	506:525	porcine brain tissue	506:525	Here, we demonstrate that it is possible to replicate the complex mechanical behavior of soft tissues, exemplary shown for porcine brain tissue, under multiple loading conditions, compression, tension, and torsional shear, through simple blends of alginate and gelatin hydrogels.
32854073	3	54	theme	alginate	631:638	arg1	blends					621:626	simple blends	614:626	simple blends of alginate and gelatin hydrogels	614:660	Here, we demonstrate that it is possible to replicate the complex mechanical behavior of soft tissues, exemplary shown for porcine brain tissue, under multiple loading conditions, compression, tension, and torsional shear, through simple blends of alginate and gelatin hydrogels.
32854073	2	55	theme	tissues	357:363	arg1	complex					298:304	complex	298:304	complex	298:304	However, the successful creation of functional tissue equivalents requires the comprehensive understanding of the complex and nonlinear mechanical properties of both native tissues and biomaterials.
32854073	2	55	theme	tissues	357:363	arg1	properties					331:340	nonlinear mechanical properties	310:340	nonlinear mechanical properties	310:340	However, the successful creation of functional tissue equivalents requires the comprehensive understanding of the complex and nonlinear mechanical properties of both native tissues and biomaterials.
32854073	0	56	theme	mechanical	47:56	arg1	behavior					58:65	the same complex mechanical behavior	30:65	the same complex mechanical behavior as brain tissue	30:81	Alginate-based hydrogels show the same complex mechanical behavior as brain tissue.
32854073	9	57	theme	component	1361:1369	arg1	importance					1340:1349	the importance	1336:1349	the importance of single component	1336:1369	Our systematic approach highlights the importance of single component, multi-modal mechanical analysis of hydrogels to understand the distinct structure-mechanics relation of each hydrogel component to eventually mimic the response of native tissues.
32854073	2	58	theme	successful	197:206	arg1	creation					208:215	the successful creation	193:215	the successful creation of functional tissue equivalents	193:248	However, the successful creation of functional tissue equivalents requires the comprehensive understanding of the complex and nonlinear mechanical properties of both native tissues and biomaterials.
32854073	1	59	theme	native	123:128	arg1	tissues					136:142	native human tissues	123:142	native human tissues	123:142	Mimicking the mechanical properties of native human tissues is one key route in tissue engineering.
32854073	10	60	theme	broad	1654:1658	arg1	applications					1690:1701	tissue engineering applications	1671:1701	tissue engineering applications	1671:1701	The presented dataset will allow for the structurally derived compositional design of hydrogels for a broad variety of tissue engineering applications.
32854073	10	60	theme	broad	1654:1658	arg1	variety					1660:1666	a broad variety	1652:1666	a broad variety of tissue engineering applications	1652:1701	The presented dataset will allow for the structurally derived compositional design of hydrogels for a broad variety of tissue engineering applications.
32854073	3	61	theme	simple	614:619	arg1	blends					621:626	simple blends	614:626	simple blends of alginate and gelatin hydrogels	614:660	Here, we demonstrate that it is possible to replicate the complex mechanical behavior of soft tissues, exemplary shown for porcine brain tissue, under multiple loading conditions, compression, tension, and torsional shear, through simple blends of alginate and gelatin hydrogels.
32854073	2	62	theme	comprehensive	263:275	arg1	understanding					277:289	the comprehensive understanding	259:289	the comprehensive understanding of the complex and nonlinear mechanical properties of both native tissues and biomaterials	259:380	However, the successful creation of functional tissue equivalents requires the comprehensive understanding of the complex and nonlinear mechanical properties of both native tissues and biomaterials.
32854073	6	63	theme	relaxation	1092:1101	arg1	behavior					1103:1110	the stiffness and loading-mode-specific stress relaxation behavior	1045:1110	the stiffness and loading-mode-specific stress relaxation behavior	1045:1110	We demonstrate that hydrogel concentration and incubation effectively tune the stiffness and loading-mode-specific stress relaxation behavior.
32854073	1	64	theme	human	130:134	arg1	tissues					136:142	native human tissues	123:142	native human tissues	123:142	Mimicking the mechanical properties of native human tissues is one key route in tissue engineering.
32854073	1	65	theme	mechanical	98:107	arg1	properties					109:118	the mechanical properties	94:118	the mechanical properties of native human tissues	94:142	Mimicking the mechanical properties of native human tissues is one key route in tissue engineering.
32854073	4	66	theme	linear	780:785	arg1	response					787:794	an almost linear response	770:794	an almost linear response	770:794	Alginate exhibits a pronounced compression-tension asymmetry and a nonlinear behavior, while gelatin shows an almost linear response.
32854073	9	67	theme	multi-modal	1372:1382	arg1	analysis					1395:1402	multi-modal mechanical analysis	1372:1402	multi-modal mechanical analysis of hydrogels	1372:1415	Our systematic approach highlights the importance of single component, multi-modal mechanical analysis of hydrogels to understand the distinct structure-mechanics relation of each hydrogel component to eventually mimic the response of native tissues.
32854073	3	68	theme	loading	543:549	arg1	conditions					551:560	multiple loading conditions	534:560	multiple loading conditions	534:560	Here, we demonstrate that it is possible to replicate the complex mechanical behavior of soft tissues, exemplary shown for porcine brain tissue, under multiple loading conditions, compression, tension, and torsional shear, through simple blends of alginate and gelatin hydrogels.
32854073	1	69	theme	tissues	136:142	arg1	properties					109:118	the mechanical properties	94:118	the mechanical properties of native human tissues	94:142	Mimicking the mechanical properties of native human tissues is one key route in tissue engineering.
32854073	3	70	dep	alginate	631:638	arg1	hydrogels					652:660	hydrogels	652:660	hydrogels	652:660	Here, we demonstrate that it is possible to replicate the complex mechanical behavior of soft tissues, exemplary shown for porcine brain tissue, under multiple loading conditions, compression, tension, and torsional shear, through simple blends of alginate and gelatin hydrogels.
32854073	0	71	theme	brain	70:74	arg1	tissue					76:81	brain tissue	70:81	brain tissue	70:81	Alginate-based hydrogels show the same complex mechanical behavior as brain tissue.
32854073	9	72	theme	structure-mechanics	1444:1462	arg1	relation					1464:1471	the distinct structure-mechanics relation	1431:1471	the distinct structure-mechanics relation of each hydrogel component to eventually mimic the response of native tissues	1431:1549	Our systematic approach highlights the importance of single component, multi-modal mechanical analysis of hydrogels to understand the distinct structure-mechanics relation of each hydrogel component to eventually mimic the response of native tissues.
32854073	4	73	theme	pronounced	683:692	arg1	asymmetry					714:722	a pronounced compression-tension asymmetry	681:722	a pronounced compression-tension asymmetry	681:722	Alginate exhibits a pronounced compression-tension asymmetry and a nonlinear behavior, while gelatin shows an almost linear response.
32854073	3	74	theme	mechanical	449:458	arg1	behavior					460:467	the complex mechanical behavior	437:467	the complex mechanical behavior of soft tissues, exemplary shown for porcine brain tissue	437:525	Here, we demonstrate that it is possible to replicate the complex mechanical behavior of soft tissues, exemplary shown for porcine brain tissue, under multiple loading conditions, compression, tension, and torsional shear, through simple blends of alginate and gelatin hydrogels.
32854073	6	75	theme	stress	1085:1090	arg1	behavior					1103:1110	the stiffness and loading-mode-specific stress relaxation behavior	1045:1110	the stiffness and loading-mode-specific stress relaxation behavior	1045:1110	We demonstrate that hydrogel concentration and incubation effectively tune the stiffness and loading-mode-specific stress relaxation behavior.
32854073	10	76	theme	applications	1690:1701	arg1	applications					1690:1701	tissue engineering applications	1671:1701	tissue engineering applications	1671:1701	The presented dataset will allow for the structurally derived compositional design of hydrogels for a broad variety of tissue engineering applications.
32854073	10	76	theme	applications	1690:1701	arg1	variety					1660:1666	a broad variety	1652:1666	a broad variety of tissue engineering applications	1652:1701	The presented dataset will allow for the structurally derived compositional design of hydrogels for a broad variety of tissue engineering applications.
32854073	2	77	theme	equivalents	238:248	arg1	creation					208:215	the successful creation	193:215	the successful creation of functional tissue equivalents	193:248	However, the successful creation of functional tissue equivalents requires the comprehensive understanding of the complex and nonlinear mechanical properties of both native tissues and biomaterials.
32854073	8	78	theme	incubation	1283:1292	arg1	times					1294:1298	long incubation times	1278:1298	long incubation times	1278:1298	In addition, we observe slower stress relaxation after long incubation times.
32854073	9	79	theme	native	1536:1541	arg1	tissues					1543:1549	native tissues	1536:1549	native tissues	1536:1549	Our systematic approach highlights the importance of single component, multi-modal mechanical analysis of hydrogels to understand the distinct structure-mechanics relation of each hydrogel component to eventually mimic the response of native tissues.
32854073	6	80	theme	loading-mode-specific	1063:1083	arg1	behavior					1103:1110	the stiffness and loading-mode-specific stress relaxation behavior	1045:1110	the stiffness and loading-mode-specific stress relaxation behavior	1045:1110	We demonstrate that hydrogel concentration and incubation effectively tune the stiffness and loading-mode-specific stress relaxation behavior.
34418755	4	0	theme	PVA	994:996	arg1	feature					968:974	the hydrophilic feature	952:974	the hydrophilic feature of both MXene and PVA	952:996	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	4	0	theme	PVA	994:996	arg1	architecture					935:946	the CF porous architecture	921:946	the CF porous architecture	921:946	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	3	1	theme	PVA	707:709	arg1	network					711:717	the porous PVA network	696:717	the porous PVA network generated inside the pores of the filter during the freeze-drying process	696:791	The presence of MXene is to absorb solar light and convert solar energy to thermal energy for efficient water evaporation, while the porous PVA network generated inside the pores of the filter during the freeze-drying process accommodates the dispersed MXene sheets and interconnects the CF and MXene.
34418755	5	2	theme	outstanding	1448:1458	arg1	efficiency					1472:1481	an outstanding evaporation efficiency	1445:1481	an outstanding evaporation efficiency of 132.9%	1445:1491	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	5	3	theme	solar	1337:1341	arg1	efficiency					1360:1369	the solar steam generation efficiency	1333:1369	the solar steam generation efficiency	1333:1369	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	5	4	theme	incident	1175:1182	arg1	light					1190:1194	the incident solar light	1171:1194	the incident solar light	1171:1194	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	4	5	theme	CF	925:926	arg1	architecture					935:946	the CF porous architecture	921:946	the CF porous architecture	921:946	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	5	6	theme	evaporation	1460:1470	arg1	efficiency					1472:1481	an outstanding evaporation efficiency	1445:1481	an outstanding evaporation efficiency of 132.9%	1445:1491	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	5	7	theme	water	1398:1402	arg1	rate					1416:1419	an ultrahigh water evaporation rate	1385:1419	an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%	1385:1491	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	4	8	theme	MXene/PVA	1013:1021	arg1	CF					1032:1033	the resultant MXene/PVA modified CF	999:1033	the resultant MXene/PVA modified CF (MPCF)	999:1040	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	4	8	theme	MXene/PVA	1013:1021	arg1	hydrophilic					1052:1062	hydrophilic	1052:1062	hydrophilic	1052:1062	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	4	8	theme	MXene/PVA	1013:1021	arg1	MPCF					1036:1039	MPCF	1036:1039	MPCF	1036:1039	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	5	9	theme	thermal	1235:1241	arg1	energy					1243:1248	thermal energy	1235:1248	thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency	1235:1369	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	4	10	theme	porous	928:933	arg1	architecture					935:946	the CF porous architecture	921:946	the CF porous architecture	921:946	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	4	11	theme	hydrophilic	956:966	arg1	feature					968:974	the hydrophilic feature	952:974	the hydrophilic feature of both MXene and PVA	952:996	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	4	12	theme	constructed	884:894	arg1	network					906:912	the constructed PVA/MXene network	880:912	the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA	880:996	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	5	13	theme	evaporation	1404:1414	arg1	rate					1416:1419	an ultrahigh water evaporation rate	1385:1419	an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%	1385:1491	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	1	14	theme	solar	277:281	arg1	efficiency					300:309	the solar steam generation efficiency	273:309	the solar steam generation efficiency	273:309	Although solar energy is promising for water purification, there is still a room for further improving the solar steam generation efficiency.
34418755	3	15	theme	MXene	583:587	arg1	presence					571:578	The presence	567:578	The presence of MXene	567:587	The presence of MXene is to absorb solar light and convert solar energy to thermal energy for efficient water evaporation, while the porous PVA network generated inside the pores of the filter during the freeze-drying process accommodates the dispersed MXene sheets and interconnects the CF and MXene.
34418755	5	16	theme	convection	1292:1301	arg1	forms					1278:1282	the forms	1274:1282	the forms of heat convection and heat radiation	1274:1320	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	1	17	theme	steam	283:287	arg1	efficiency					300:309	the solar steam generation efficiency	273:309	the solar steam generation efficiency	273:309	Although solar energy is promising for water purification, there is still a room for further improving the solar steam generation efficiency.
34418755	0	18	theme	solar	135:139	arg1	efficiency					158:167	ultrahigh solar steam generation efficiency	125:167	ultrahigh solar steam generation efficiency	125:167	An environmental energy-enhanced solar steam evaporator derived from MXene-decorated cellulose acetate cigarette filter with ultrahigh solar steam generation efficiency.
34418755	5	19	theme	3.38 kg m-2	1424:1434	arg1	h-1					1436:1438	3.38 kg m-2 h-1	1424:1438	3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%	1424:1491	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	1	20	theme	water	209:213	arg1	purification					215:226	water purification	209:226	water purification	209:226	Although solar energy is promising for water purification, there is still a room for further improving the solar steam generation efficiency.
34418755	2	21	theme	cigarette	435:443	arg1	CF					453:454	CF	453:454	CF	453:454	Herein, an environmental energy-enhanced solar steam evaporator is fabricated by immersing a cellulose acetate fiber-based cigarette filter (CF) in an aqueous solution of polyvinyl alcohol (PVA) followed by freeze-drying and decorating with MXene sheets.
34418755	2	21	theme	cigarette	435:443	arg1	filter					445:450	a cellulose acetate fiber-based cigarette filter	403:450	a cellulose acetate fiber-based cigarette filter (CF)	403:455	Herein, an environmental energy-enhanced solar steam evaporator is fabricated by immersing a cellulose acetate fiber-based cigarette filter (CF) in an aqueous solution of polyvinyl alcohol (PVA) followed by freeze-drying and decorating with MXene sheets.
34418755	0	22	theme	ultrahigh	125:133	arg1	efficiency					158:167	ultrahigh solar steam generation efficiency	125:167	ultrahigh solar steam generation efficiency	125:167	An environmental energy-enhanced solar steam evaporator derived from MXene-decorated cellulose acetate cigarette filter with ultrahigh solar steam generation efficiency.
34418755	5	23	theme	h-1	1436:1438	arg1	rate					1416:1419	an ultrahigh water evaporation rate	1385:1419	an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%	1385:1491	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	4	24	theme	modified	1023:1030	arg1	CF					1032:1033	the resultant MXene/PVA modified CF	999:1033	the resultant MXene/PVA modified CF (MPCF)	999:1040	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	4	24	theme	modified	1023:1030	arg1	hydrophilic					1052:1062	hydrophilic	1052:1062	hydrophilic	1052:1062	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	4	24	theme	modified	1023:1030	arg1	MPCF					1036:1039	MPCF	1036:1039	MPCF	1036:1039	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	5	25	theme	heat	1307:1310	arg1	radiation					1312:1320	heat radiation	1307:1320	heat radiation	1307:1320	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	2	26	theme	fiber-based	423:433	arg1	CF					453:454	CF	453:454	CF	453:454	Herein, an environmental energy-enhanced solar steam evaporator is fabricated by immersing a cellulose acetate fiber-based cigarette filter (CF) in an aqueous solution of polyvinyl alcohol (PVA) followed by freeze-drying and decorating with MXene sheets.
34418755	2	26	theme	fiber-based	423:433	arg1	filter					445:450	a cellulose acetate fiber-based cigarette filter	403:450	a cellulose acetate fiber-based cigarette filter (CF)	403:455	Herein, an environmental energy-enhanced solar steam evaporator is fabricated by immersing a cellulose acetate fiber-based cigarette filter (CF) in an aqueous solution of polyvinyl alcohol (PVA) followed by freeze-drying and decorating with MXene sheets.
34418755	0	27	theme	environmental	3:15	arg1	evaporator					45:54	An environmental energy-enhanced solar steam evaporator	0:54	An environmental energy-enhanced solar steam evaporator derived from MXene-decorated cellulose acetate	0:101	An environmental energy-enhanced solar steam evaporator derived from MXene-decorated cellulose acetate cigarette filter with ultrahigh solar steam generation efficiency.
34418755	5	28	from	environment	1259:1269	arg1	energy					1243:1248	thermal energy	1235:1248	thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency	1235:1369	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	5	28	from	environment	1259:1269	arg1	forms					1278:1282	the forms	1274:1282	the forms of heat convection and heat radiation	1274:1320	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	4	29	theme	MXene	984:988	arg1	feature					968:974	the hydrophilic feature	952:974	the hydrophilic feature of both MXene and PVA	952:996	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	4	29	theme	MXene	984:988	arg1	architecture					935:946	the CF porous architecture	921:946	the CF porous architecture	921:946	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	2	30	theme	energy-enhanced	337:351	arg1	evaporator					365:374	an environmental energy-enhanced solar steam evaporator	320:374	an environmental energy-enhanced solar steam evaporator	320:374	Herein, an environmental energy-enhanced solar steam evaporator is fabricated by immersing a cellulose acetate fiber-based cigarette filter (CF) in an aqueous solution of polyvinyl alcohol (PVA) followed by freeze-drying and decorating with MXene sheets.
34418755	5	31	theme	%	1491:1491	arg1	efficiency					1472:1481	an outstanding evaporation efficiency	1445:1481	an outstanding evaporation efficiency of 132.9%	1445:1491	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	2	32	theme	acetate	415:421	arg1	CF					453:454	CF	453:454	CF	453:454	Herein, an environmental energy-enhanced solar steam evaporator is fabricated by immersing a cellulose acetate fiber-based cigarette filter (CF) in an aqueous solution of polyvinyl alcohol (PVA) followed by freeze-drying and decorating with MXene sheets.
34418755	2	32	theme	acetate	415:421	arg1	filter					445:450	a cellulose acetate fiber-based cigarette filter	403:450	a cellulose acetate fiber-based cigarette filter (CF)	403:455	Herein, an environmental energy-enhanced solar steam evaporator is fabricated by immersing a cellulose acetate fiber-based cigarette filter (CF) in an aqueous solution of polyvinyl alcohol (PVA) followed by freeze-drying and decorating with MXene sheets.
34418755	2	33	theme	aqueous	463:469	arg1	solution					471:478	an aqueous solution	460:478	an aqueous solution of polyvinyl alcohol (PVA) followed by freeze-drying	460:531	Herein, an environmental energy-enhanced solar steam evaporator is fabricated by immersing a cellulose acetate fiber-based cigarette filter (CF) in an aqueous solution of polyvinyl alcohol (PVA) followed by freeze-drying and decorating with MXene sheets.
34418755	1	34	theme	generation	289:298	arg1	efficiency					300:309	the solar steam generation efficiency	273:309	the solar steam generation efficiency	273:309	Although solar energy is promising for water purification, there is still a room for further improving the solar steam generation efficiency.
34418755	0	35	attach	derived	56:62	arg1	acetate					95:101	MXene-decorated cellulose acetate	69:101	MXene-decorated cellulose acetate	69:101	An environmental energy-enhanced solar steam evaporator derived from MXene-decorated cellulose acetate cigarette filter with ultrahigh solar steam generation efficiency.
34418755	0	35	attach	derived	56:62	arg2	evaporator					45:54	An environmental energy-enhanced solar steam evaporator	0:54	An environmental energy-enhanced solar steam evaporator derived from MXene-decorated cellulose acetate	0:101	An environmental energy-enhanced solar steam evaporator derived from MXene-decorated cellulose acetate cigarette filter with ultrahigh solar steam generation efficiency.
34418755	2	36	theme	environmental	323:335	arg1	evaporator					365:374	an environmental energy-enhanced solar steam evaporator	320:374	an environmental energy-enhanced solar steam evaporator	320:374	Herein, an environmental energy-enhanced solar steam evaporator is fabricated by immersing a cellulose acetate fiber-based cigarette filter (CF) in an aqueous solution of polyvinyl alcohol (PVA) followed by freeze-drying and decorating with MXene sheets.
34418755	2	37	theme	cellulose	405:413	arg1	CF					453:454	CF	453:454	CF	453:454	Herein, an environmental energy-enhanced solar steam evaporator is fabricated by immersing a cellulose acetate fiber-based cigarette filter (CF) in an aqueous solution of polyvinyl alcohol (PVA) followed by freeze-drying and decorating with MXene sheets.
34418755	2	37	theme	cellulose	405:413	arg1	filter					445:450	a cellulose acetate fiber-based cigarette filter	403:450	a cellulose acetate fiber-based cigarette filter (CF)	403:455	Herein, an environmental energy-enhanced solar steam evaporator is fabricated by immersing a cellulose acetate fiber-based cigarette filter (CF) in an aqueous solution of polyvinyl alcohol (PVA) followed by freeze-drying and decorating with MXene sheets.
34418755	0	38	theme	solar	33:37	arg1	evaporator					45:54	An environmental energy-enhanced solar steam evaporator	0:54	An environmental energy-enhanced solar steam evaporator derived from MXene-decorated cellulose acetate	0:101	An environmental energy-enhanced solar steam evaporator derived from MXene-decorated cellulose acetate cigarette filter with ultrahigh solar steam generation efficiency.
34418755	3	39	theme	filter	753:758	arg1	pores					740:744	the pores	736:744	the pores of the filter	736:758	The presence of MXene is to absorb solar light and convert solar energy to thermal energy for efficient water evaporation, while the porous PVA network generated inside the pores of the filter during the freeze-drying process accommodates the dispersed MXene sheets and interconnects the CF and MXene.
34418755	0	40	theme	generation	147:156	arg1	efficiency					158:167	ultrahigh solar steam generation efficiency	125:167	ultrahigh solar steam generation efficiency	125:167	An environmental energy-enhanced solar steam evaporator derived from MXene-decorated cellulose acetate cigarette filter with ultrahigh solar steam generation efficiency.
34418755	5	41	theme	ultrahigh	1388:1396	arg1	rate					1416:1419	an ultrahigh water evaporation rate	1385:1419	an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%	1385:1491	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	4	42	theme	upward	1088:1093	arg1	transfer					1095:1102	rapid upward transfer	1082:1102	rapid upward transfer of water	1082:1111	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	0	43	theme	energy-enhanced	17:31	arg1	evaporator					45:54	An environmental energy-enhanced solar steam evaporator	0:54	An environmental energy-enhanced solar steam evaporator derived from MXene-decorated cellulose acetate	0:101	An environmental energy-enhanced solar steam evaporator derived from MXene-decorated cellulose acetate cigarette filter with ultrahigh solar steam generation efficiency.
34418755	4	44	theme	resultant	1003:1011	arg1	CF					1032:1033	the resultant MXene/PVA modified CF	999:1033	the resultant MXene/PVA modified CF (MPCF)	999:1040	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	4	44	theme	resultant	1003:1011	arg1	hydrophilic					1052:1062	hydrophilic	1052:1062	hydrophilic	1052:1062	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	4	44	theme	resultant	1003:1011	arg1	MPCF					1036:1039	MPCF	1036:1039	MPCF	1036:1039	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	0	45	theme	steam	141:145	arg1	efficiency					158:167	ultrahigh solar steam generation efficiency	125:167	ultrahigh solar steam generation efficiency	125:167	An environmental energy-enhanced solar steam evaporator derived from MXene-decorated cellulose acetate cigarette filter with ultrahigh solar steam generation efficiency.
34418755	4	46	theme	water	1107:1111	arg1	transfer					1095:1102	rapid upward transfer	1082:1102	rapid upward transfer of water	1082:1111	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	3	47	theme	solar	602:606	arg1	light					608:612	solar light	602:612	solar light	602:612	The presence of MXene is to absorb solar light and convert solar energy to thermal energy for efficient water evaporation, while the porous PVA network generated inside the pores of the filter during the freeze-drying process accommodates the dispersed MXene sheets and interconnects the CF and MXene.
34418755	5	48	theme	heat	1287:1290	arg1	convection					1292:1301	heat convection	1287:1301	heat convection	1287:1301	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	5	49	theme	radiation	1312:1320	arg1	forms					1278:1282	the forms	1274:1282	the forms of heat convection and heat radiation	1274:1320	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	0	50	theme	steam	39:43	arg1	evaporator					45:54	An environmental energy-enhanced solar steam evaporator	0:54	An environmental energy-enhanced solar steam evaporator derived from MXene-decorated cellulose acetate	0:101	An environmental energy-enhanced solar steam evaporator derived from MXene-decorated cellulose acetate cigarette filter with ultrahigh solar steam generation efficiency.
34418755	3	51	theme	freeze-drying	771:783	arg1	process					785:791	the freeze-drying process	767:791	the freeze-drying process	767:791	The presence of MXene is to absorb solar light and convert solar energy to thermal energy for efficient water evaporation, while the porous PVA network generated inside the pores of the filter during the freeze-drying process accommodates the dispersed MXene sheets and interconnects the CF and MXene.
34418755	2	52	theme	alcohol	493:499	arg1	solution					471:478	an aqueous solution	460:478	an aqueous solution of polyvinyl alcohol (PVA) followed by freeze-drying	460:531	Herein, an environmental energy-enhanced solar steam evaporator is fabricated by immersing a cellulose acetate fiber-based cigarette filter (CF) in an aqueous solution of polyvinyl alcohol (PVA) followed by freeze-drying and decorating with MXene sheets.
34418755	5	53	theme	steam	1343:1347	arg1	efficiency					1360:1369	the solar steam generation efficiency	1333:1369	the solar steam generation efficiency	1333:1369	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	5	54	theme	normal	1148:1153	arg1	input					1162:1166	the normal energy input	1144:1166	the normal energy input	1144:1166	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	2	55	theme	polyvinyl	483:491	arg1	PVA					502:504	PVA	502:504	PVA	502:504	Herein, an environmental energy-enhanced solar steam evaporator is fabricated by immersing a cellulose acetate fiber-based cigarette filter (CF) in an aqueous solution of polyvinyl alcohol (PVA) followed by freeze-drying and decorating with MXene sheets.
34418755	2	55	theme	polyvinyl	483:491	arg1	alcohol					493:499	polyvinyl alcohol	483:499	polyvinyl alcohol (PVA) followed by freeze-drying	483:531	Herein, an environmental energy-enhanced solar steam evaporator is fabricated by immersing a cellulose acetate fiber-based cigarette filter (CF) in an aqueous solution of polyvinyl alcohol (PVA) followed by freeze-drying and decorating with MXene sheets.
34418755	4	56	theme	network	906:912	arg1	CF					1032:1033	the resultant MXene/PVA modified CF	999:1033	the resultant MXene/PVA modified CF (MPCF)	999:1040	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	4	56	theme	network	906:912	arg1	hydrophilic					1052:1062	hydrophilic	1052:1062	hydrophilic	1052:1062	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	3	57	theme	thermal	642:648	arg1	energy					650:655	thermal energy	642:655	thermal energy for efficient water evaporation	642:687	The presence of MXene is to absorb solar light and convert solar energy to thermal energy for efficient water evaporation, while the porous PVA network generated inside the pores of the filter during the freeze-drying process accommodates the dispersed MXene sheets and interconnects the CF and MXene.
34418755	5	58	theme	generation	1349:1358	arg1	efficiency					1360:1369	the solar steam generation efficiency	1333:1369	the solar steam generation efficiency	1333:1369	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	2	59	theme	MXene	553:557	arg1	sheets					559:564	MXene sheets	553:564	MXene sheets	553:564	Herein, an environmental energy-enhanced solar steam evaporator is fabricated by immersing a cellulose acetate fiber-based cigarette filter (CF) in an aqueous solution of polyvinyl alcohol (PVA) followed by freeze-drying and decorating with MXene sheets.
34418755	4	60	theme	PVA/MXene	896:904	arg1	network					906:912	the constructed PVA/MXene network	880:912	the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA	880:996	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	5	61	theme	large-area	1201:1210	arg1	sidewall					1212:1219	the large-area sidewall	1197:1219	the large-area sidewall of MPCF	1197:1227	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	3	62	theme	solar	626:630	arg1	energy					632:637	solar energy	626:637	solar energy	626:637	The presence of MXene is to absorb solar light and convert solar energy to thermal energy for efficient water evaporation, while the porous PVA network generated inside the pores of the filter during the freeze-drying process accommodates the dispersed MXene sheets and interconnects the CF and MXene.
34418755	2	63	theme	steam	359:363	arg1	evaporator					365:374	an environmental energy-enhanced solar steam evaporator	320:374	an environmental energy-enhanced solar steam evaporator	320:374	Herein, an environmental energy-enhanced solar steam evaporator is fabricated by immersing a cellulose acetate fiber-based cigarette filter (CF) in an aqueous solution of polyvinyl alcohol (PVA) followed by freeze-drying and decorating with MXene sheets.
34418755	3	64	theme	dispersed	810:818	arg1	sheets					826:831	the dispersed MXene sheets	806:831	the dispersed MXene sheets	806:831	The presence of MXene is to absorb solar light and convert solar energy to thermal energy for efficient water evaporation, while the porous PVA network generated inside the pores of the filter during the freeze-drying process accommodates the dispersed MXene sheets and interconnects the CF and MXene.
34418755	3	65	theme	efficient	661:669	arg1	evaporation					677:687	efficient water evaporation	661:687	efficient water evaporation	661:687	The presence of MXene is to absorb solar light and convert solar energy to thermal energy for efficient water evaporation, while the porous PVA network generated inside the pores of the filter during the freeze-drying process accommodates the dispersed MXene sheets and interconnects the CF and MXene.
34418755	5	66	with	h-1	1436:1438	arg1	efficiency					1472:1481	an outstanding evaporation efficiency	1445:1481	an outstanding evaporation efficiency of 132.9%	1445:1491	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	2	67	theme	solar	353:357	arg1	evaporator					365:374	an environmental energy-enhanced solar steam evaporator	320:374	an environmental energy-enhanced solar steam evaporator	320:374	Herein, an environmental energy-enhanced solar steam evaporator is fabricated by immersing a cellulose acetate fiber-based cigarette filter (CF) in an aqueous solution of polyvinyl alcohol (PVA) followed by freeze-drying and decorating with MXene sheets.
34418755	4	68	theme	rapid	1082:1086	arg1	transfer					1095:1102	rapid upward transfer	1082:1102	rapid upward transfer of water	1082:1111	Because of the constructed PVA/MXene network inside the CF porous architecture and the hydrophilic feature of both MXene and PVA, the resultant MXene/PVA modified CF (MPCF) is highly hydrophilic and competent for rapid upward transfer of water.
34418755	3	69	theme	MXene	820:824	arg1	sheets					826:831	the dispersed MXene sheets	806:831	the dispersed MXene sheets	806:831	The presence of MXene is to absorb solar light and convert solar energy to thermal energy for efficient water evaporation, while the porous PVA network generated inside the pores of the filter during the freeze-drying process accommodates the dispersed MXene sheets and interconnects the CF and MXene.
34418755	0	70	theme	cellulose	85:93	arg1	acetate					95:101	MXene-decorated cellulose acetate	69:101	MXene-decorated cellulose acetate	69:101	An environmental energy-enhanced solar steam evaporator derived from MXene-decorated cellulose acetate cigarette filter with ultrahigh solar steam generation efficiency.
34418755	3	71	theme	water	671:675	arg1	evaporation					677:687	efficient water evaporation	661:687	efficient water evaporation	661:687	The presence of MXene is to absorb solar light and convert solar energy to thermal energy for efficient water evaporation, while the porous PVA network generated inside the pores of the filter during the freeze-drying process accommodates the dispersed MXene sheets and interconnects the CF and MXene.
34418755	5	72	theme	energy	1155:1160	arg1	input					1162:1166	the normal energy input	1144:1166	the normal energy input	1144:1166	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	0	73	theme	MXene-decorated	69:83	arg1	acetate					95:101	MXene-decorated cellulose acetate	69:101	MXene-decorated cellulose acetate	69:101	An environmental energy-enhanced solar steam evaporator derived from MXene-decorated cellulose acetate cigarette filter with ultrahigh solar steam generation efficiency.
34418755	3	74	theme	porous	700:705	arg1	network					711:717	the porous PVA network	696:717	the porous PVA network generated inside the pores of the filter during the freeze-drying process	696:791	The presence of MXene is to absorb solar light and convert solar energy to thermal energy for efficient water evaporation, while the porous PVA network generated inside the pores of the filter during the freeze-drying process accommodates the dispersed MXene sheets and interconnects the CF and MXene.
34418755	1	75	theme	solar	179:183	arg1	energy					185:190	solar energy	179:190	solar energy	179:190	Although solar energy is promising for water purification, there is still a room for further improving the solar steam generation efficiency.
34418755	5	76	theme	MPCF	1224:1227	arg1	sidewall					1212:1219	the large-area sidewall	1197:1219	the large-area sidewall of MPCF	1197:1227	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
34418755	5	77	theme	solar	1184:1188	arg1	light					1190:1194	the incident solar light	1171:1194	the incident solar light	1171:1194	Interestingly, in addition to the normal energy input by the incident solar light, the large-area sidewall of MPCF gains thermal energy from the environment in the forms of heat convection and heat radiation to enhance the solar steam generation efficiency, resulting in an ultrahigh water evaporation rate of 3.38 kg m-2 h-1 with an outstanding evaporation efficiency of 132.9%.
33690032	6	0	dep	100	1263:1265	arg1	to					1260:1261	to	1260:1261	to	1260:1261	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	3	1	theme	physical	673:680	arg1	stability					682:690	the physical stability	669:690	the physical stability	669:690	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC was investigated.
33690032	1	2	theme	wastewater	262:271	arg1	treatment					249:257	high-rate treatment	239:257	high-rate treatment of wastewater	239:271	Alginate can be used for entrapment of microalgal cells in gel beads to achieve high-rate treatment of wastewater and can overcome the difficulties of cell separation that would occur in suspended microalgae treatment systems.
33690032	6	3	dep	TP	1268:1269	arg1	%					1266:1266	%	1266:1266	%	1266:1266	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	8	4	dep	insight	1639:1645	arg1	the					1635:1637	the	1635:1637	the	1635:1637	This study enhances the insight and decision-making regarding the feasibility of the alginate-immobilised microalgal system for treating municipal wastewater ROC streams.
33690032	4	5	theme	calcium	896:902	arg1	loss					888:891	the loss	884:891	the loss of calcium from the alginate matrix	884:927	Water adsorption resulting from the loss of calcium from the alginate matrix was the initiating cause of reduction of the algal bead stability.
33690032	1	6	used	used	175:178	arg2	Alginate					159:166	Alginate	159:166	Alginate	159:166	Alginate can be used for entrapment of microalgal cells in gel beads to achieve high-rate treatment of wastewater and can overcome the difficulties of cell separation that would occur in suspended microalgae treatment systems.
33690032	3	7	theme	biomass	719:725	arg1	production					727:736	biomass production	719:736	biomass production	719:736	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC was investigated.
33690032	3	8	theme	characteristics	702:716	arg1	performance					760:770	the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance	649:770	the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC	649:832	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC was investigated.
33690032	6	9	theme	alginate	1160:1167	arg1	beads					1169:1173	alginate beads	1160:1173	alginate beads of C. vulgaris	1160:1188	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	3	10	theme	nutrient	743:750	arg1	removal					752:758	nutrient removal	743:758	nutrient removal	743:758	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC was investigated.
33690032	1	11	theme	microalgal	198:207	arg1	cells					209:213	microalgal cells	198:213	microalgal cells	198:213	Alginate can be used for entrapment of microalgal cells in gel beads to achieve high-rate treatment of wastewater and can overcome the difficulties of cell separation that would occur in suspended microalgae treatment systems.
33690032	7	12	theme	algal	1573:1577	arg1	beads					1579:1583	the algal beads	1569:1583	the algal beads	1569:1583	Empirical models that were developed and validated could enable the prediction of the performance of the algal beads for various ROC compositions.
33690032	6	13	theme	48 h	1305:1308	arg1	cycle					1296:1300	treatment cycle	1286:1300	treatment cycle of 48 h over a 10-day period	1286:1329	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	2	14	theme	osmosis	563:569	arg1	concentrate					571:581	municipal wastewater reverse osmosis concentrate	534:581	municipal wastewater reverse osmosis concentrate (ROC)	534:587	The potential for alginate beads to disintegrate in the presence of high ion concentrations could limit the use of alginate entrapment for treating municipal wastewater reverse osmosis concentrate (ROC).
33690032	2	14	theme	osmosis	563:569	arg1	ROC					584:586	ROC	584:586	ROC	584:586	The potential for alginate beads to disintegrate in the presence of high ion concentrations could limit the use of alginate entrapment for treating municipal wastewater reverse osmosis concentrate (ROC).
33690032	6	15	theme	remediation	1244:1254	arg1	capable					1224:1230	capable	1224:1230	capable	1224:1230	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	3	16	theme	alginate-entrapped	775:792	arg1	vulgaris					804:811	alginate-entrapped Chlorella vulgaris	775:811	alginate-entrapped Chlorella vulgaris for treating the ROC	775:832	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC was investigated.
33690032	5	17	theme	compressive	1075:1085	arg1	strength					1087:1094	compressive strength	1075:1094	compressive strength	1075:1094	The combination of alkalinity >400 mg/L and pH ≥9.5 led to a >65% reduction in compressive strength and thus disintegration of beads during ROC treatment.
33690032	6	18	theme	salinity	1379:1386	arg1	levels					1408:1413	salinity, pH, and alkalinity levels	1379:1413	salinity, pH, and alkalinity levels	1379:1413	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	3	19	theme	performance	760:770	arg1	effect					603:608	The combined effect	590:608	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC	590:832	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC was investigated.
33690032	4	20	from	matrix	922:927	arg1	loss					888:891	the loss	884:891	the loss of calcium from the alginate matrix	884:927	Water adsorption resulting from the loss of calcium from the alginate matrix was the initiating cause of reduction of the algal bead stability.
33690032	8	21	theme	microalgal	1721:1730	arg1	system					1732:1737	the alginate-immobilised microalgal system	1696:1737	the alginate-immobilised microalgal system for treating municipal wastewater ROC streams	1696:1783	This study enhances the insight and decision-making regarding the feasibility of the alginate-immobilised microalgal system for treating municipal wastewater ROC streams.
33690032	6	22	theme	%	1277:1277	arg1	TN					1279:1280	85% TN	1275:1280	85% TN	1275:1280	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	5	23	theme	>65	1057:1059	arg1	%					1060:1060	%	1060:1060	%	1060:1060	The combination of alkalinity >400 mg/L and pH ≥9.5 led to a >65% reduction in compressive strength and thus disintegration of beads during ROC treatment.
33690032	2	24	theme	alginate	501:508	arg1	entrapment					510:519	alginate entrapment	501:519	alginate entrapment	501:519	The potential for alginate beads to disintegrate in the presence of high ion concentrations could limit the use of alginate entrapment for treating municipal wastewater reverse osmosis concentrate (ROC).
33690032	4	25	theme	bead	980:983	arg1	stability					985:993	the algal bead stability	970:993	the algal bead stability	970:993	Water adsorption resulting from the loss of calcium from the alginate matrix was the initiating cause of reduction of the algal bead stability.
33690032	3	26	theme	vulgaris	804:811	arg1	performance					760:770	the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance	649:770	the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC	649:832	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC was investigated.
33690032	6	27	theme	production	1344:1353	arg1	capable					1224:1230	capable	1224:1230	capable	1224:1230	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	5	28	theme	beads	1123:1127	arg1	disintegration					1105:1118	disintegration	1105:1118	a >65% reduction in compressive strength and thus disintegration of beads during ROC treatment	1055:1148	The combination of alkalinity >400 mg/L and pH ≥9.5 led to a >65% reduction in compressive strength and thus disintegration of beads during ROC treatment.
33690032	8	29	theme	municipal	1752:1760	arg1	streams					1777:1783	municipal wastewater ROC streams	1752:1783	municipal wastewater ROC streams	1752:1783	This study enhances the insight and decision-making regarding the feasibility of the alginate-immobilised microalgal system for treating municipal wastewater ROC streams.
33690032	6	30	dep	remediation	1244:1254	arg1	TP					1268:1269	up to 100% TP	1257:1269	up to 100% TP	1257:1269	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	6	30	dep	remediation	1244:1254	arg1	TN					1279:1280	85% TN	1275:1280	85% TN	1275:1280	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	0	31	theme	Integrated	91:100	arg1	impact					102:107	Integrated impact	91:107	Integrated impact of solution conditions on algal bead performance	91:156	Treatment of wastewater reverse osmosis concentrate using alginate-immobilised microalgae: Integrated impact of solution conditions on algal bead performance.
33690032	1	32	theme	suspended	346:354	arg1	systems					377:383	suspended microalgae treatment systems	346:383	suspended microalgae treatment systems	346:383	Alginate can be used for entrapment of microalgal cells in gel beads to achieve high-rate treatment of wastewater and can overcome the difficulties of cell separation that would occur in suspended microalgae treatment systems.
33690032	6	33	theme	10-day	1317:1322	arg1	period					1324:1329	a 10-day period	1315:1329	a 10-day period	1315:1329	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	4	34	theme	reduction	957:965	arg1	adsorption					858:867	Water adsorption	852:867	Water adsorption resulting from the loss of calcium from the alginate matrix	852:927	Water adsorption resulting from the loss of calcium from the alginate matrix was the initiating cause of reduction of the algal bead stability.
33690032	4	34	theme	reduction	957:965	arg1	cause					948:952	the initiating cause	933:952	the initiating cause of reduction of the algal bead stability	933:993	Water adsorption resulting from the loss of calcium from the alginate matrix was the initiating cause of reduction of the algal bead stability.
33690032	1	35	theme	treatment	367:375	arg1	systems					377:383	suspended microalgae treatment systems	346:383	suspended microalgae treatment systems	346:383	Alginate can be used for entrapment of microalgal cells in gel beads to achieve high-rate treatment of wastewater and can overcome the difficulties of cell separation that would occur in suspended microalgae treatment systems.
33690032	0	36	theme	conditions	121:130	arg1	impact					102:107	Integrated impact	91:107	Integrated impact of solution conditions on algal bead performance	91:156	Treatment of wastewater reverse osmosis concentrate using alginate-immobilised microalgae: Integrated impact of solution conditions on algal bead performance.
33690032	2	37	theme	ion	459:461	arg1	concentrations					463:476	high ion concentrations	454:476	high ion concentrations	454:476	The potential for alginate beads to disintegrate in the presence of high ion concentrations could limit the use of alginate entrapment for treating municipal wastewater reverse osmosis concentrate (ROC).
33690032	2	38	theme	entrapment	510:519	arg1	use					494:496	the use	490:496	the use of alginate entrapment for treating municipal wastewater reverse osmosis concentrate (ROC)	490:587	The potential for alginate beads to disintegrate in the presence of high ion concentrations could limit the use of alginate entrapment for treating municipal wastewater reverse osmosis concentrate (ROC).
33690032	7	39	theme	various	1589:1595	arg1	compositions					1601:1612	various ROC compositions	1589:1612	various ROC compositions	1589:1612	Empirical models that were developed and validated could enable the prediction of the performance of the algal beads for various ROC compositions.
33690032	0	40	theme	wastewater	13:22	arg1	osmosis					32:38	wastewater reverse osmosis	13:38	wastewater reverse osmosis	13:38	Treatment of wastewater reverse osmosis concentrate using alginate-immobilised microalgae: Integrated impact of solution conditions on algal bead performance.
33690032	7	41	theme	beads	1579:1583	arg1	performance					1554:1564	the performance	1550:1564	the performance of the algal beads	1550:1583	Empirical models that were developed and validated could enable the prediction of the performance of the algal beads for various ROC compositions.
33690032	0	42	theme	osmosis	32:38	arg1	Treatment					0:8	Treatment	0:8	Treatment of wastewater reverse osmosis	0:38	Treatment of wastewater reverse osmosis concentrate using alginate-immobilised microalgae: Integrated impact of solution conditions on algal bead performance.
33690032	1	43	theme	cell	310:313	arg1	separation					315:324	cell separation	310:324	cell separation	310:324	Alginate can be used for entrapment of microalgal cells in gel beads to achieve high-rate treatment of wastewater and can overcome the difficulties of cell separation that would occur in suspended microalgae treatment systems.
33690032	6	44	dep	340 mg/L/d	1362:1371	arg1	to					1359:1360	to	1359:1360	to	1359:1360	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	3	45	theme	salinity	637:644	arg1	effect					603:608	The combined effect	590:608	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC	590:832	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC was investigated.
33690032	1	46	theme	high-rate	239:247	arg1	treatment					249:257	high-rate treatment	239:257	high-rate treatment of wastewater	239:271	Alginate can be used for entrapment of microalgal cells in gel beads to achieve high-rate treatment of wastewater and can overcome the difficulties of cell separation that would occur in suspended microalgae treatment systems.
33690032	5	47	theme	alkalinity	1015:1024	arg1	>400 mg/L					1026:1034	alkalinity >400 mg/L	1015:1034	alkalinity >400 mg/L	1015:1034	The combination of alkalinity >400 mg/L and pH ≥9.5 led to a >65% reduction in compressive strength and thus disintegration of beads during ROC treatment.
33690032	3	48	theme	chemical	693:700	arg1	characteristics					702:716	chemical characteristics	693:716	chemical characteristics	693:716	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC was investigated.
33690032	6	49	theme	85	1275:1276	arg1	%					1277:1277	%	1277:1277	%	1277:1277	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	6	50	theme	alkalinity	1397:1406	arg1	levels					1408:1413	salinity, pH, and alkalinity levels	1379:1413	salinity, pH, and alkalinity levels	1379:1413	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	6	51	dep	%	1266:1266	arg1	100					1263:1265	100	1263:1265	100	1263:1265	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	6	52	theme	C. vulgaris	1178:1188	arg1	beads					1169:1173	alginate beads	1160:1173	alginate beads of C. vulgaris	1160:1188	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	3	53	theme	production	727:736	arg1	performance					760:770	the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance	649:770	the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC	649:832	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC was investigated.
33690032	2	54	theme	concentrations	463:476	arg1	presence					442:449	the presence	438:449	the presence of high ion concentrations	438:476	The potential for alginate beads to disintegrate in the presence of high ion concentrations could limit the use of alginate entrapment for treating municipal wastewater reverse osmosis concentrate (ROC).
33690032	4	55	theme	Water	852:856	arg1	adsorption					858:867	Water adsorption	852:867	Water adsorption resulting from the loss of calcium from the alginate matrix	852:927	Water adsorption resulting from the loss of calcium from the alginate matrix was the initiating cause of reduction of the algal bead stability.
33690032	4	55	theme	Water	852:856	arg1	cause					948:952	the initiating cause	933:952	the initiating cause of reduction of the algal bead stability	933:993	Water adsorption resulting from the loss of calcium from the alginate matrix was the initiating cause of reduction of the algal bead stability.
33690032	5	56	theme	ROC	1136:1138	arg1	treatment					1140:1148	ROC treatment	1136:1148	ROC treatment	1136:1148	The combination of alkalinity >400 mg/L and pH ≥9.5 led to a >65% reduction in compressive strength and thus disintegration of beads during ROC treatment.
33690032	3	57	theme	removal	752:758	arg1	performance					760:770	the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance	649:770	the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC	649:832	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC was investigated.
33690032	0	58	theme	bead	141:144	arg1	performance					146:156	algal bead performance	135:156	algal bead performance	135:156	Treatment of wastewater reverse osmosis concentrate using alginate-immobilised microalgae: Integrated impact of solution conditions on algal bead performance.
33690032	8	59	theme	ROC	1773:1775	arg1	streams					1777:1783	municipal wastewater ROC streams	1752:1783	municipal wastewater ROC streams	1752:1783	This study enhances the insight and decision-making regarding the feasibility of the alginate-immobilised microalgal system for treating municipal wastewater ROC streams.
33690032	1	60	theme	cells	209:213	arg1	entrapment					184:193	entrapment	184:193	entrapment of microalgal cells in gel beads	184:226	Alginate can be used for entrapment of microalgal cells in gel beads to achieve high-rate treatment of wastewater and can overcome the difficulties of cell separation that would occur in suspended microalgae treatment systems.
33690032	7	61	theme	performance	1554:1564	arg1	prediction					1536:1545	the prediction	1532:1545	the prediction of the performance of the algal beads for various ROC compositions	1532:1612	Empirical models that were developed and validated could enable the prediction of the performance of the algal beads for various ROC compositions.
33690032	0	62	from	impact	102:107	arg1	performance					146:156	algal bead performance	135:156	algal bead performance	135:156	Treatment of wastewater reverse osmosis concentrate using alginate-immobilised microalgae: Integrated impact of solution conditions on algal bead performance.
33690032	1	63	theme	gel	218:220	arg1	beads					222:226	gel beads	218:226	gel beads	218:226	Alginate can be used for entrapment of microalgal cells in gel beads to achieve high-rate treatment of wastewater and can overcome the difficulties of cell separation that would occur in suspended microalgae treatment systems.
33690032	4	64	theme	initiating	937:946	arg1	adsorption					858:867	Water adsorption	852:867	Water adsorption resulting from the loss of calcium from the alginate matrix	852:927	Water adsorption resulting from the loss of calcium from the alginate matrix was the initiating cause of reduction of the algal bead stability.
33690032	4	64	theme	initiating	937:946	arg1	cause					948:952	the initiating cause	933:952	the initiating cause of reduction of the algal bead stability	933:993	Water adsorption resulting from the loss of calcium from the alginate matrix was the initiating cause of reduction of the algal bead stability.
33690032	6	65	theme	nutrient	1235:1242	arg1	remediation					1244:1254	nutrient remediation	1235:1254	nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period)	1235:1330	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	2	66	theme	wastewater	544:553	arg1	concentrate					571:581	municipal wastewater reverse osmosis concentrate	534:581	municipal wastewater reverse osmosis concentrate (ROC)	534:587	The potential for alginate beads to disintegrate in the presence of high ion concentrations could limit the use of alginate entrapment for treating municipal wastewater reverse osmosis concentrate (ROC).
33690032	2	66	theme	wastewater	544:553	arg1	ROC					584:586	ROC	584:586	ROC	584:586	The potential for alginate beads to disintegrate in the presence of high ion concentrations could limit the use of alginate entrapment for treating municipal wastewater reverse osmosis concentrate (ROC).
33690032	6	67	theme	treatment	1286:1294	arg1	cycle					1296:1300	treatment cycle	1286:1300	treatment cycle of 48 h over a 10-day period	1286:1329	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	3	68	theme	combined	594:601	arg1	effect					603:608	The combined effect	590:608	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC	590:832	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC was investigated.
33690032	3	69	theme	alkalinity	621:630	arg1	effect					603:608	The combined effect	590:608	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC	590:832	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC was investigated.
33690032	8	70	theme	alginate-immobilised	1700:1719	arg1	system					1732:1737	the alginate-immobilised microalgal system	1696:1737	the alginate-immobilised microalgal system for treating municipal wastewater ROC streams	1696:1783	This study enhances the insight and decision-making regarding the feasibility of the alginate-immobilised microalgal system for treating municipal wastewater ROC streams.
33690032	4	71	theme	alginate	913:920	arg1	matrix					922:927	the alginate matrix	909:927	the alginate matrix	909:927	Water adsorption resulting from the loss of calcium from the alginate matrix was the initiating cause of reduction of the algal bead stability.
33690032	5	72	theme	%	1060:1060	arg1	reduction					1062:1070	a >65% reduction	1055:1070	a >65% reduction in compressive strength and thus disintegration of beads during ROC treatment	1055:1148	The combination of alkalinity >400 mg/L and pH ≥9.5 led to a >65% reduction in compressive strength and thus disintegration of beads during ROC treatment.
33690032	3	73	theme	pH	617:618	arg1	effect					603:608	The combined effect	590:608	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC	590:832	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC was investigated.
33690032	8	74	theme	system	1732:1737	arg1	feasibility					1681:1691	the feasibility	1677:1691	the feasibility of the alginate-immobilised microalgal system for treating municipal wastewater ROC streams	1677:1783	This study enhances the insight and decision-making regarding the feasibility of the alginate-immobilised microalgal system for treating municipal wastewater ROC streams.
33690032	4	75	theme	algal	974:978	arg1	stability					985:993	the algal bead stability	970:993	the algal bead stability	970:993	Water adsorption resulting from the loss of calcium from the alginate matrix was the initiating cause of reduction of the algal bead stability.
33690032	6	76	theme	biomass	1336:1342	arg1	340 mg/L/d					1362:1371	340 mg/L/d	1362:1371	340 mg/L/d	1362:1371	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	6	76	theme	biomass	1336:1342	arg1	production					1344:1353	biomass production	1336:1353	biomass production (up to 340 mg/L/d)	1336:1372	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	1	77	from	entrapment	184:193	arg1	beads					222:226	gel beads	218:226	gel beads	218:226	Alginate can be used for entrapment of microalgal cells in gel beads to achieve high-rate treatment of wastewater and can overcome the difficulties of cell separation that would occur in suspended microalgae treatment systems.
33690032	4	78	theme	stability	985:993	arg1	reduction					957:965	reduction	957:965	reduction of the algal bead stability	957:993	Water adsorption resulting from the loss of calcium from the alginate matrix was the initiating cause of reduction of the algal bead stability.
33690032	0	79	theme	solution	112:119	arg1	conditions					121:130	solution conditions	112:130	solution conditions	112:130	Treatment of wastewater reverse osmosis concentrate using alginate-immobilised microalgae: Integrated impact of solution conditions on algal bead performance.
33690032	1	80	theme	microalgae	356:365	arg1	systems					377:383	suspended microalgae treatment systems	346:383	suspended microalgae treatment systems	346:383	Alginate can be used for entrapment of microalgal cells in gel beads to achieve high-rate treatment of wastewater and can overcome the difficulties of cell separation that would occur in suspended microalgae treatment systems.
33690032	8	81	theme	wastewater	1762:1771	arg1	streams					1777:1783	municipal wastewater ROC streams	1752:1783	municipal wastewater ROC streams	1752:1783	This study enhances the insight and decision-making regarding the feasibility of the alginate-immobilised microalgal system for treating municipal wastewater ROC streams.
33690032	2	82	theme	municipal	534:542	arg1	concentrate					571:581	municipal wastewater reverse osmosis concentrate	534:581	municipal wastewater reverse osmosis concentrate (ROC)	534:587	The potential for alginate beads to disintegrate in the presence of high ion concentrations could limit the use of alginate entrapment for treating municipal wastewater reverse osmosis concentrate (ROC).
33690032	2	82	theme	municipal	534:542	arg1	ROC					584:586	ROC	584:586	ROC	584:586	The potential for alginate beads to disintegrate in the presence of high ion concentrations could limit the use of alginate entrapment for treating municipal wastewater reverse osmosis concentrate (ROC).
33690032	5	83	from	reduction	1062:1070	arg1	strength					1087:1094	compressive strength	1075:1094	compressive strength	1075:1094	The combination of alkalinity >400 mg/L and pH ≥9.5 led to a >65% reduction in compressive strength and thus disintegration of beads during ROC treatment.
33690032	2	84	theme	alginate	404:411	arg1	beads					413:417	alginate beads	404:417	alginate beads	404:417	The potential for alginate beads to disintegrate in the presence of high ion concentrations could limit the use of alginate entrapment for treating municipal wastewater reverse osmosis concentrate (ROC).
33690032	6	85	theme	pH	1389:1390	arg1	levels					1408:1413	salinity, pH, and alkalinity levels	1379:1413	salinity, pH, and alkalinity levels	1379:1413	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	2	86	theme	high	454:457	arg1	concentrations					463:476	high ion concentrations	454:476	high ion concentrations	454:476	The potential for alginate beads to disintegrate in the presence of high ion concentrations could limit the use of alginate entrapment for treating municipal wastewater reverse osmosis concentrate (ROC).
33690032	0	87	theme	reverse	24:30	arg1	osmosis					32:38	wastewater reverse osmosis	13:38	wastewater reverse osmosis	13:38	Treatment of wastewater reverse osmosis concentrate using alginate-immobilised microalgae: Integrated impact of solution conditions on algal bead performance.
33690032	7	88	theme	ROC	1597:1599	arg1	compositions					1601:1612	various ROC compositions	1589:1612	various ROC compositions	1589:1612	Empirical models that were developed and validated could enable the prediction of the performance of the algal beads for various ROC compositions.
33690032	7	89	theme	Empirical	1468:1476	arg1	models					1478:1483	Empirical models	1468:1483	Empirical models that were developed and validated	1468:1517	Empirical models that were developed and validated could enable the prediction of the performance of the algal beads for various ROC compositions.
33690032	5	90	theme	>400 mg/L	1026:1034	arg1	combination					1000:1010	The combination	996:1010	The combination of alkalinity >400 mg/L and pH ≥9.5	996:1046	The combination of alkalinity >400 mg/L and pH ≥9.5 led to a >65% reduction in compressive strength and thus disintegration of beads during ROC treatment.
33690032	0	91	theme	alginate-immobilised	58:77	arg1	microalgae					79:88	alginate-immobilised microalgae	58:88	alginate-immobilised microalgae	58:88	Treatment of wastewater reverse osmosis concentrate using alginate-immobilised microalgae: Integrated impact of solution conditions on algal bead performance.
33690032	1	92	theme	separation	315:324	arg1	difficulties					294:305	the difficulties	290:305	the difficulties of cell separation that would occur in suspended microalgae treatment systems	290:383	Alginate can be used for entrapment of microalgal cells in gel beads to achieve high-rate treatment of wastewater and can overcome the difficulties of cell separation that would occur in suspended microalgae treatment systems.
33690032	5	93	theme	pH ≥9.5	1040:1046	arg1	combination					1000:1010	The combination	996:1010	The combination of alkalinity >400 mg/L and pH ≥9.5	996:1046	The combination of alkalinity >400 mg/L and pH ≥9.5 led to a >65% reduction in compressive strength and thus disintegration of beads during ROC treatment.
33690032	2	94	from	potential	390:398	arg1	presence					442:449	the presence	438:449	the presence of high ion concentrations	438:476	The potential for alginate beads to disintegrate in the presence of high ion concentrations could limit the use of alginate entrapment for treating municipal wastewater reverse osmosis concentrate (ROC).
33690032	2	95	theme	reverse	555:561	arg1	concentrate					571:581	municipal wastewater reverse osmosis concentrate	534:581	municipal wastewater reverse osmosis concentrate (ROC)	534:587	The potential for alginate beads to disintegrate in the presence of high ion concentrations could limit the use of alginate entrapment for treating municipal wastewater reverse osmosis concentrate (ROC).
33690032	2	95	theme	reverse	555:561	arg1	ROC					584:586	ROC	584:586	ROC	584:586	The potential for alginate beads to disintegrate in the presence of high ion concentrations could limit the use of alginate entrapment for treating municipal wastewater reverse osmosis concentrate (ROC).
33690032	3	96	theme	ROC	653:655	arg1	performance					760:770	the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance	649:770	the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC	649:832	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC was investigated.
33690032	6	97	theme	<8 g	1420:1423	arg1	TDS/L					1425:1429	<8 g TDS/L	1420:1429	<8 g TDS/L	1420:1429	However, alginate beads of C. vulgaris were sufficiently stable and were capable of nutrient remediation (up to 100% TP and 85% TN per treatment cycle of 48 h over a 10-day period) and biomass production (up to 340 mg/L/d) when salinity, pH, and alkalinity levels were <8 g TDS/L, 7-9.5, and <400 mg/L, respectively.
33690032	0	98	theme	algal	135:139	arg1	performance					146:156	algal bead performance	135:156	algal bead performance	135:156	Treatment of wastewater reverse osmosis concentrate using alginate-immobilised microalgae: Integrated impact of solution conditions on algal bead performance.
33690032	3	99	theme	Chlorella	794:802	arg1	vulgaris					804:811	alginate-entrapped Chlorella vulgaris	775:811	alginate-entrapped Chlorella vulgaris for treating the ROC	775:832	The combined effect of the pH, alkalinity, and salinity of the ROC that impact the physical stability, chemical characteristics, biomass production, and nutrient removal performance of alginate-entrapped Chlorella vulgaris for treating the ROC was investigated.
31965348	0	0	theme	skull	93:97	arg1	defects					99:105	rat skull defects	89:105	rat skull defects	89:105	Study on osteogenesis of zinc-loaded carbon nanotubes/chitosan composite biomaterials in rat skull defects.
31965348	0	1	from	osteogenesis	9:20	arg1	defects					99:105	rat skull defects	89:105	rat skull defects	89:105	Study on osteogenesis of zinc-loaded carbon nanotubes/chitosan composite biomaterials in rat skull defects.
31965348	7	2	theme	transplanted	1100:1111	arg1	surgeries					1113:1121	transplanted surgeries	1100:1121	transplanted surgeries	1100:1121	The experimental animals were executed at 12 weeks after transplanted surgeries, and the rat skull defects were removed for related analyses.
31965348	5	3	theme	%	820:820	arg1	nanotubes/Chitosan					834:851	0.2% Zinc-Carbon nanotubes/Chitosan	817:851	0.2% Zinc-Carbon nanotubes/Chitosan (GL)	817:856	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	5	3	theme	%	820:820	arg1	GL					854:855	GL	854:855	GL	854:855	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	4	4	theme	rat	695:697	arg1	defects					699:705	rat defects	695:705	rat defects	695:705	The purpose of the current research was to investigate the effects of Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials in the restoration of rat skull defects, and to verify the hypothesis that these zinc ions of appropriate concentration would strengthen the osteogenesis of rat defects.
31965348	10	5	theme	satisfactory	1514:1525	arg1	osteogenesis					1527:1538	satisfactory osteogenesis	1514:1538	satisfactory osteogenesis	1514:1538	Overall, these composite biomaterials revealed satisfactory osteogenesis, nevertheless, there was a requirement to further perfect the zinc ion concentrations to achieve the better bone regeneration.
31965348	6	6	theme	composite	973:981	arg1	biomaterials					983:994	these composite biomaterials	967:994	these composite biomaterials	967:994	After characterizations, these composite biomaterials were then transplanted into rat skull defects.
31965348	0	7	theme	rat	89:91	arg1	defects					99:105	rat skull defects	89:105	rat skull defects	89:105	Study on osteogenesis of zinc-loaded carbon nanotubes/chitosan composite biomaterials in rat skull defects.
31965348	4	8	theme	Nanotubes/Chitosan	496:513	arg1	biomaterials					525:536	Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials	477:536	Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials	477:536	The purpose of the current research was to investigate the effects of Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials in the restoration of rat skull defects, and to verify the hypothesis that these zinc ions of appropriate concentration would strengthen the osteogenesis of rat defects.
31965348	4	9	theme	research	434:441	arg1	purpose					411:417	The purpose	407:417	The purpose of the current research	407:441	The purpose of the current research was to investigate the effects of Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials in the restoration of rat skull defects, and to verify the hypothesis that these zinc ions of appropriate concentration would strengthen the osteogenesis of rat defects.
31965348	10	10	theme	zinc	1602:1605	arg1	concentrations					1611:1624	the zinc ion concentrations	1598:1624	the zinc ion concentrations	1598:1624	Overall, these composite biomaterials revealed satisfactory osteogenesis, nevertheless, there was a requirement to further perfect the zinc ion concentrations to achieve the better bone regeneration.
31965348	4	11	theme	defects	570:576	arg1	restoration					545:555	the restoration	541:555	the restoration of rat skull defects	541:576	The purpose of the current research was to investigate the effects of Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials in the restoration of rat skull defects, and to verify the hypothesis that these zinc ions of appropriate concentration would strengthen the osteogenesis of rat defects.
31965348	4	12	theme	Carbon	489:494	arg1	biomaterials					525:536	Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials	477:536	Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials	477:536	The purpose of the current research was to investigate the effects of Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials in the restoration of rat skull defects, and to verify the hypothesis that these zinc ions of appropriate concentration would strengthen the osteogenesis of rat defects.
31965348	3	13	dep	development	364:374	arg1	the					360:362	the	360:362	the	360:362	Zinc, an essential trace element, contributes to the development and growth of skeletal system.
31965348	9	14	theme	Zinc-Carbon	1435:1445	arg1	nanotubes/Chitosan					1447:1464	1% Zinc-Carbon nanotubes/Chitosan	1432:1464	1% Zinc-Carbon nanotubes/Chitosan	1432:1464	An important finding was that the optimal osteogenic effect appeared in rat skull defects transplanted with 1% Zinc-Carbon nanotubes/Chitosan.
31965348	3	15	theme	trace	330:334	arg1	element					336:342	an essential trace element	317:342	an essential trace element	317:342	Zinc, an essential trace element, contributes to the development and growth of skeletal system.
31965348	3	15	theme	trace	330:334	arg1	Zinc					311:314	Zinc	311:314	Zinc	311:314	Zinc, an essential trace element, contributes to the development and growth of skeletal system.
31965348	8	16	theme	osteoinductive	1297:1310	arg1	properties					1312:1321	good mechanical and osteoinductive properties	1277:1321	good mechanical and osteoinductive properties	1277:1321	The results of characterizations suggested the Zinc-loaded composite biomaterials possessed good mechanical and osteoinductive properties.
31965348	4	17	from	effects	466:472	arg1	restoration					545:555	the restoration	541:555	the restoration of rat skull defects	541:576	The purpose of the current research was to investigate the effects of Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials in the restoration of rat skull defects, and to verify the hypothesis that these zinc ions of appropriate concentration would strengthen the osteogenesis of rat defects.
31965348	6	18	theme	skull	1028:1032	arg1	defects					1034:1040	rat skull defects	1024:1040	rat skull defects	1024:1040	After characterizations, these composite biomaterials were then transplanted into rat skull defects.
31965348	4	19	theme	concentration	644:656	arg1	ions					624:627	these zinc ions	613:627	these zinc ions of appropriate concentration	613:656	The purpose of the current research was to investigate the effects of Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials in the restoration of rat skull defects, and to verify the hypothesis that these zinc ions of appropriate concentration would strengthen the osteogenesis of rat defects.
31965348	5	20	theme	biomaterials	743:754	arg1	groups					723:728	Four different groups	708:728	Four different groups of composite biomaterials	708:754	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	5	20	theme	biomaterials	743:754	arg1	biomaterials					743:754	composite biomaterials	733:754	composite biomaterials	733:754	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	3	21	theme	skeletal	390:397	arg1	system					399:404	skeletal system	390:404	skeletal system	390:404	Zinc, an essential trace element, contributes to the development and growth of skeletal system.
31965348	4	22	theme	appropriate	632:642	arg1	concentration					644:656	appropriate concentration	632:656	appropriate concentration	632:656	The purpose of the current research was to investigate the effects of Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials in the restoration of rat skull defects, and to verify the hypothesis that these zinc ions of appropriate concentration would strengthen the osteogenesis of rat defects.
31965348	1	23	with	Chitosan	108:115	arg1	polymer					160:166	a natural polymer	150:166	a natural polymer	150:166	Chitosan with hydroxyapatite composition, a natural polymer, may be a biomaterial of importance for bone regeneration.
31965348	1	23	with	Chitosan	108:115	arg1	composition					137:147	hydroxyapatite composition	122:147	hydroxyapatite composition	122:147	Chitosan with hydroxyapatite composition, a natural polymer, may be a biomaterial of importance for bone regeneration.
31965348	9	24	theme	osteogenic	1366:1375	arg1	effect					1377:1382	the optimal osteogenic effect	1354:1382	the optimal osteogenic effect	1354:1382	An important finding was that the optimal osteogenic effect appeared in rat skull defects transplanted with 1% Zinc-Carbon nanotubes/Chitosan.
31965348	5	25	theme	%	903:903	arg1	nanotubes/Chitosan					917:934	2% Zinc-Carbon nanotubes/Chitosan	902:934	2% Zinc-Carbon nanotubes/Chitosan (GH)	902:939	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	5	25	theme	%	903:903	arg1	GH					937:938	GH	937:938	GH	937:938	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	9	26	theme	important	1327:1335	arg1	finding					1337:1343	An important finding	1324:1343	An important finding	1324:1343	An important finding was that the optimal osteogenic effect appeared in rat skull defects transplanted with 1% Zinc-Carbon nanotubes/Chitosan.
31965348	8	27	theme	composite	1244:1252	arg1	biomaterials					1254:1265	the Zinc-loaded composite biomaterials	1228:1265	the Zinc-loaded composite biomaterials	1228:1265	The results of characterizations suggested the Zinc-loaded composite biomaterials possessed good mechanical and osteoinductive properties.
31965348	10	28	theme	better	1641:1646	arg1	regeneration					1653:1664	the better bone regeneration	1637:1664	the better bone regeneration	1637:1664	Overall, these composite biomaterials revealed satisfactory osteogenesis, nevertheless, there was a requirement to further perfect the zinc ion concentrations to achieve the better bone regeneration.
31965348	7	29	theme	rat	1132:1134	arg1	defects					1142:1148	the rat skull defects	1128:1148	the rat skull defects	1128:1148	The experimental animals were executed at 12 weeks after transplanted surgeries, and the rat skull defects were removed for related analyses.
31965348	10	30	theme	composite	1482:1490	arg1	biomaterials					1492:1503	these composite biomaterials	1476:1503	these composite biomaterials	1476:1503	Overall, these composite biomaterials revealed satisfactory osteogenesis, nevertheless, there was a requirement to further perfect the zinc ion concentrations to achieve the better bone regeneration.
31965348	5	31	theme	Zinc-Carbon	905:915	arg1	nanotubes/Chitosan					917:934	2% Zinc-Carbon nanotubes/Chitosan	902:934	2% Zinc-Carbon nanotubes/Chitosan (GH)	902:939	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	5	31	theme	Zinc-Carbon	905:915	arg1	GH					937:938	GH	937:938	GH	937:938	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	5	32	theme	1	859:859	arg1	%					860:860	%	860:860	%	860:860	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	7	33	theme	skull	1136:1140	arg1	defects					1142:1148	the rat skull defects	1128:1148	the rat skull defects	1128:1148	The experimental animals were executed at 12 weeks after transplanted surgeries, and the rat skull defects were removed for related analyses.
31965348	7	34	theme	experimental	1047:1058	arg1	animals					1060:1066	The experimental animals	1043:1066	The experimental animals	1043:1066	The experimental animals were executed at 12 weeks after transplanted surgeries, and the rat skull defects were removed for related analyses.
31965348	2	35	theme	bone	293:296	arg1	restoration					298:308	bone restoration	293:308	bone restoration	293:308	Carbon nanotube, a nanoscale material, has been another focus for bone restoration.
31965348	1	36	theme	importance	193:202	arg1	biomaterial					178:188	a biomaterial	176:188	a biomaterial of importance for bone regeneration	176:224	Chitosan with hydroxyapatite composition, a natural polymer, may be a biomaterial of importance for bone regeneration.
31965348	1	36	theme	importance	193:202	arg1	Chitosan					108:115	Chitosan	108:115	Chitosan with hydroxyapatite composition, a natural polymer,	108:167	Chitosan with hydroxyapatite composition, a natural polymer, may be a biomaterial of importance for bone regeneration.
31965348	5	37	theme	0.2	817:819	arg1	%					820:820	%	820:820	%	820:820	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	4	38	theme	skull	564:568	arg1	defects					570:576	rat skull defects	560:576	rat skull defects	560:576	The purpose of the current research was to investigate the effects of Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials in the restoration of rat skull defects, and to verify the hypothesis that these zinc ions of appropriate concentration would strengthen the osteogenesis of rat defects.
31965348	4	39	theme	current	426:432	arg1	research					434:441	the current research	422:441	the current research	422:441	The purpose of the current research was to investigate the effects of Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials in the restoration of rat skull defects, and to verify the hypothesis that these zinc ions of appropriate concentration would strengthen the osteogenesis of rat defects.
31965348	8	40	dep	suggested	1218:1226	arg1	possessed					1267:1275	possessed	1267:1275	suggested the Zinc-loaded composite biomaterials possessed good mechanical and osteoinductive properties	1218:1321	The results of characterizations suggested the Zinc-loaded composite biomaterials possessed good mechanical and osteoinductive properties.
31965348	5	41	theme	%	860:860	arg1	nanotubes/Chitosan					874:891	1% Zinc-Carbon nanotubes/Chitosan	859:891	1% Zinc-Carbon nanotubes/Chitosan (GM)	859:896	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	5	41	theme	%	860:860	arg1	GM					894:895	GM	894:895	GM	894:895	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	5	42	theme	Zinc-Carbon	822:832	arg1	nanotubes/Chitosan					834:851	0.2% Zinc-Carbon nanotubes/Chitosan	817:851	0.2% Zinc-Carbon nanotubes/Chitosan (GL)	817:856	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	5	42	theme	Zinc-Carbon	822:832	arg1	GL					854:855	GL	854:855	GL	854:855	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	0	43	theme	carbon	37:42	arg1	biomaterials					73:84	zinc-loaded carbon nanotubes/chitosan composite biomaterials	25:84	zinc-loaded carbon nanotubes/chitosan composite biomaterials	25:84	Study on osteogenesis of zinc-loaded carbon nanotubes/chitosan composite biomaterials in rat skull defects.
31965348	4	44	theme	rat	560:562	arg1	defects					570:576	rat skull defects	560:576	rat skull defects	560:576	The purpose of the current research was to investigate the effects of Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials in the restoration of rat skull defects, and to verify the hypothesis that these zinc ions of appropriate concentration would strengthen the osteogenesis of rat defects.
31965348	6	45	theme	rat	1024:1026	arg1	defects					1034:1040	rat skull defects	1024:1040	rat skull defects	1024:1040	After characterizations, these composite biomaterials were then transplanted into rat skull defects.
31965348	5	46	theme	different	713:721	arg1	groups					723:728	Four different groups	708:728	Four different groups of composite biomaterials	708:754	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	5	46	theme	different	713:721	arg1	biomaterials					743:754	composite biomaterials	733:754	composite biomaterials	733:754	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	8	47	theme	Zinc-loaded	1232:1242	arg1	biomaterials					1254:1265	the Zinc-loaded composite biomaterials	1228:1265	the Zinc-loaded composite biomaterials	1228:1265	The results of characterizations suggested the Zinc-loaded composite biomaterials possessed good mechanical and osteoinductive properties.
31965348	1	48	theme	bone	208:211	arg1	regeneration					213:224	bone regeneration	208:224	bone regeneration	208:224	Chitosan with hydroxyapatite composition, a natural polymer, may be a biomaterial of importance for bone regeneration.
31965348	0	49	theme	zinc-loaded	25:35	arg1	biomaterials					73:84	zinc-loaded carbon nanotubes/chitosan composite biomaterials	25:84	zinc-loaded carbon nanotubes/chitosan composite biomaterials	25:84	Study on osteogenesis of zinc-loaded carbon nanotubes/chitosan composite biomaterials in rat skull defects.
31965348	4	50	theme	biomaterials	525:536	arg1	effects					466:472	the effects	462:472	the effects of Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials in the restoration of rat skull defects	462:576	The purpose of the current research was to investigate the effects of Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials in the restoration of rat skull defects, and to verify the hypothesis that these zinc ions of appropriate concentration would strengthen the osteogenesis of rat defects.
31965348	3	51	theme	system	399:404	arg1	development					364:374	development	364:374	development	364:374	Zinc, an essential trace element, contributes to the development and growth of skeletal system.
31965348	3	51	theme	system	399:404	arg1	growth					380:385	growth	380:385	growth	380:385	Zinc, an essential trace element, contributes to the development and growth of skeletal system.
31965348	8	52	theme	mechanical	1282:1291	arg1	properties					1312:1321	good mechanical and osteoinductive properties	1277:1321	good mechanical and osteoinductive properties	1277:1321	The results of characterizations suggested the Zinc-loaded composite biomaterials possessed good mechanical and osteoinductive properties.
31965348	9	53	theme	1	1432:1432	arg1	%					1433:1433	%	1433:1433	%	1433:1433	An important finding was that the optimal osteogenic effect appeared in rat skull defects transplanted with 1% Zinc-Carbon nanotubes/Chitosan.
31965348	7	54	theme	related	1167:1173	arg1	analyses					1175:1182	related analyses	1167:1182	related analyses	1167:1182	The experimental animals were executed at 12 weeks after transplanted surgeries, and the rat skull defects were removed for related analyses.
31965348	0	55	theme	composite	63:71	arg1	biomaterials					73:84	zinc-loaded carbon nanotubes/chitosan composite biomaterials	25:84	zinc-loaded carbon nanotubes/chitosan composite biomaterials	25:84	Study on osteogenesis of zinc-loaded carbon nanotubes/chitosan composite biomaterials in rat skull defects.
31965348	4	56	theme	composite	515:523	arg1	biomaterials					525:536	Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials	477:536	Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials	477:536	The purpose of the current research was to investigate the effects of Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials in the restoration of rat skull defects, and to verify the hypothesis that these zinc ions of appropriate concentration would strengthen the osteogenesis of rat defects.
31965348	0	57	theme	biomaterials	73:84	arg1	osteogenesis					9:20	osteogenesis	9:20	osteogenesis of zinc-loaded carbon nanotubes/chitosan composite biomaterials in rat skull defects	9:105	Study on osteogenesis of zinc-loaded carbon nanotubes/chitosan composite biomaterials in rat skull defects.
31965348	2	58	theme	Carbon	227:232	arg1	nanotube					234:241	Carbon nanotube	227:241	Carbon nanotube	227:241	Carbon nanotube, a nanoscale material, has been another focus for bone restoration.
31965348	2	58	theme	Carbon	227:232	arg1	material					256:263	a nanoscale material	244:263	a nanoscale material	244:263	Carbon nanotube, a nanoscale material, has been another focus for bone restoration.
31965348	1	59	theme	hydroxyapatite	122:135	arg1	polymer					160:166	a natural polymer	150:166	a natural polymer	150:166	Chitosan with hydroxyapatite composition, a natural polymer, may be a biomaterial of importance for bone regeneration.
31965348	1	59	theme	hydroxyapatite	122:135	arg1	composition					137:147	hydroxyapatite composition	122:147	hydroxyapatite composition	122:147	Chitosan with hydroxyapatite composition, a natural polymer, may be a biomaterial of importance for bone regeneration.
31965348	0	60	theme	nanotubes/chitosan	44:61	arg1	biomaterials					73:84	zinc-loaded carbon nanotubes/chitosan composite biomaterials	25:84	zinc-loaded carbon nanotubes/chitosan composite biomaterials	25:84	Study on osteogenesis of zinc-loaded carbon nanotubes/chitosan composite biomaterials in rat skull defects.
31965348	4	61	theme	defects	699:705	arg1	osteogenesis					679:690	the osteogenesis	675:690	the osteogenesis of rat defects	675:705	The purpose of the current research was to investigate the effects of Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials in the restoration of rat skull defects, and to verify the hypothesis that these zinc ions of appropriate concentration would strengthen the osteogenesis of rat defects.
31965348	9	62	theme	rat	1396:1398	arg1	defects					1406:1412	rat skull defects	1396:1412	rat skull defects transplanted with 1% Zinc-Carbon nanotubes/Chitosan	1396:1464	An important finding was that the optimal osteogenic effect appeared in rat skull defects transplanted with 1% Zinc-Carbon nanotubes/Chitosan.
31965348	9	63	theme	optimal	1358:1364	arg1	effect					1377:1382	the optimal osteogenic effect	1354:1382	the optimal osteogenic effect	1354:1382	An important finding was that the optimal osteogenic effect appeared in rat skull defects transplanted with 1% Zinc-Carbon nanotubes/Chitosan.
31965348	9	64	theme	skull	1400:1404	arg1	defects					1406:1412	rat skull defects	1396:1412	rat skull defects transplanted with 1% Zinc-Carbon nanotubes/Chitosan	1396:1464	An important finding was that the optimal osteogenic effect appeared in rat skull defects transplanted with 1% Zinc-Carbon nanotubes/Chitosan.
31965348	5	65	theme	composite	733:741	arg1	biomaterials					743:754	composite biomaterials	733:754	composite biomaterials	733:754	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	5	66	theme	Zinc	780:783	arg1	nanotubes/Chitosan					792:809	no Zinc Carbon nanotubes/Chitosan	777:809	no Zinc Carbon nanotubes/Chitosan (GN)	777:814	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	5	66	theme	Zinc	780:783	arg1	GN					812:813	GN	812:813	GN	812:813	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	9	67	theme	%	1433:1433	arg1	nanotubes/Chitosan					1447:1464	1% Zinc-Carbon nanotubes/Chitosan	1432:1464	1% Zinc-Carbon nanotubes/Chitosan	1432:1464	An important finding was that the optimal osteogenic effect appeared in rat skull defects transplanted with 1% Zinc-Carbon nanotubes/Chitosan.
31965348	10	68	theme	ion	1607:1609	arg1	concentrations					1611:1624	the zinc ion concentrations	1598:1624	the zinc ion concentrations	1598:1624	Overall, these composite biomaterials revealed satisfactory osteogenesis, nevertheless, there was a requirement to further perfect the zinc ion concentrations to achieve the better bone regeneration.
31965348	5	69	theme	2	902:902	arg1	%					903:903	%	903:903	%	903:903	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	8	70	theme	characterizations	1200:1216	arg1	results					1189:1195	The results	1185:1195	The results of characterizations	1185:1216	The results of characterizations suggested the Zinc-loaded composite biomaterials possessed good mechanical and osteoinductive properties.
31965348	10	71	theme	bone	1648:1651	arg1	regeneration					1653:1664	the better bone regeneration	1637:1664	the better bone regeneration	1637:1664	Overall, these composite biomaterials revealed satisfactory osteogenesis, nevertheless, there was a requirement to further perfect the zinc ion concentrations to achieve the better bone regeneration.
31965348	8	72	theme	good	1277:1280	arg1	properties					1312:1321	good mechanical and osteoinductive properties	1277:1321	good mechanical and osteoinductive properties	1277:1321	The results of characterizations suggested the Zinc-loaded composite biomaterials possessed good mechanical and osteoinductive properties.
31965348	2	73	theme	nanoscale	246:254	arg1	nanotube					234:241	Carbon nanotube	227:241	Carbon nanotube	227:241	Carbon nanotube, a nanoscale material, has been another focus for bone restoration.
31965348	2	73	theme	nanoscale	246:254	arg1	material					256:263	a nanoscale material	244:263	a nanoscale material	244:263	Carbon nanotube, a nanoscale material, has been another focus for bone restoration.
31965348	4	74	theme	zinc	619:622	arg1	ions					624:627	these zinc ions	613:627	these zinc ions of appropriate concentration	613:656	The purpose of the current research was to investigate the effects of Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials in the restoration of rat skull defects, and to verify the hypothesis that these zinc ions of appropriate concentration would strengthen the osteogenesis of rat defects.
31965348	1	75	theme	natural	152:158	arg1	polymer					160:166	a natural polymer	150:166	a natural polymer	150:166	Chitosan with hydroxyapatite composition, a natural polymer, may be a biomaterial of importance for bone regeneration.
31965348	1	75	theme	natural	152:158	arg1	composition					137:147	hydroxyapatite composition	122:147	hydroxyapatite composition	122:147	Chitosan with hydroxyapatite composition, a natural polymer, may be a biomaterial of importance for bone regeneration.
31965348	3	76	theme	essential	320:328	arg1	element					336:342	an essential trace element	317:342	an essential trace element	317:342	Zinc, an essential trace element, contributes to the development and growth of skeletal system.
31965348	3	76	theme	essential	320:328	arg1	Zinc					311:314	Zinc	311:314	Zinc	311:314	Zinc, an essential trace element, contributes to the development and growth of skeletal system.
31965348	4	77	theme	Zinc-loaded	477:487	arg1	biomaterials					525:536	Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials	477:536	Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials	477:536	The purpose of the current research was to investigate the effects of Zinc-loaded Carbon Nanotubes/Chitosan composite biomaterials in the restoration of rat skull defects, and to verify the hypothesis that these zinc ions of appropriate concentration would strengthen the osteogenesis of rat defects.
31965348	5	78	theme	Carbon	785:790	arg1	nanotubes/Chitosan					792:809	no Zinc Carbon nanotubes/Chitosan	777:809	no Zinc Carbon nanotubes/Chitosan (GN)	777:814	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	5	78	theme	Carbon	785:790	arg1	GN					812:813	GN	812:813	GN	812:813	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	5	79	theme	Zinc-Carbon	862:872	arg1	nanotubes/Chitosan					874:891	1% Zinc-Carbon nanotubes/Chitosan	859:891	1% Zinc-Carbon nanotubes/Chitosan (GM)	859:896	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
31965348	5	79	theme	Zinc-Carbon	862:872	arg1	GM					894:895	GM	894:895	GM	894:895	Four different groups of composite biomaterials were fabricated from no Zinc Carbon nanotubes/Chitosan (GN), 0.2% Zinc-Carbon nanotubes/Chitosan (GL), 1% Zinc-Carbon nanotubes/Chitosan (GM) and 2% Zinc-Carbon nanotubes/Chitosan (GH).
34634327	4	0	theme	bionanocomposite	1008:1023	arg1	films					1025:1029	KGM/CNC bionanocomposite films	1000:1029	KGM/CNC bionanocomposite films	1000:1029	Thermogravimetric analysis and differential scanning calorimetry demonstrated that incorporating PS improved the heat stability of KGM/CNC bionanocomposite films.
34634327	1	1	theme	cellulose	375:383	arg1	composites					404:413	konjac glucomannan (KGM)/cotton cellulose nanocrystals (CNC) composites	343:413	konjac glucomannan (KGM)/cotton cellulose nanocrystals (CNC) composites	343:413	In this study, environmentally friendly bionanocomposite films were prepared by incorporating phlorotannins from Sargassum (PS) into konjac glucomannan (KGM)/cotton cellulose nanocrystals (CNC) composites.
34634327	6	2	theme	water-vapor	1289:1299	arg1	transmittance					1301:1313	the water-vapor transmittance	1285:1313	the water-vapor transmittance	1285:1313	For instance, with 9% PS, the tensile strength of the KGM/CNC/PS bionanocomposite film increased by 33.9%, and the water-vapor transmittance decreased by 41.67% compared to that of the KGM/CNC films.
34634327	7	3	theme	antioxidant	1443:1453	arg1	properties					1473:1482	excellent antioxidant and antibacterial properties	1433:1482	excellent antioxidant and antibacterial properties	1433:1482	Moreover, the addition of PS endowed the KGM/CNC film with excellent antioxidant and antibacterial properties.
34634327	3	4	theme	films	862:866	arg1	structure					845:853	a compact and uniform structure	823:853	a compact and uniform structure of the films	823:866	The results of scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectra showed that PS was well compatible with the KGM/CNC composites matrix, which led to form a compact and uniform structure of the films.
34634327	0	5	theme	antimicrobial	73:85	arg1	activity					87:94	antimicrobial activity	73:94	antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films	73:161	Investigation of the structural and physical properties, antioxidant and antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films incorporated with phlorotannin from Sargassum.
34634327	0	5	theme	antimicrobial	73:85	arg1	properties					45:54	the structural and physical properties	17:54	the structural and physical properties	17:54	Investigation of the structural and physical properties, antioxidant and antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films incorporated with phlorotannin from Sargassum.
34634327	1	6	theme	nanocrystals	385:396	arg1	composites					404:413	konjac glucomannan (KGM)/cotton cellulose nanocrystals (CNC) composites	343:413	konjac glucomannan (KGM)/cotton cellulose nanocrystals (CNC) composites	343:413	In this study, environmentally friendly bionanocomposite films were prepared by incorporating phlorotannins from Sargassum (PS) into konjac glucomannan (KGM)/cotton cellulose nanocrystals (CNC) composites.
34634327	5	7	theme	PS	1074:1075	arg1	PS					1074:1075	PS	1074:1075	PS	1074:1075	And addition of the appropriate amount of PS improved the mechanical and water-vapor barrier-related properties of the bionanocomposite film.
34634327	5	7	theme	PS	1074:1075	arg1	amount					1064:1069	the appropriate amount	1048:1069	the appropriate amount of PS	1048:1075	And addition of the appropriate amount of PS improved the mechanical and water-vapor barrier-related properties of the bionanocomposite film.
34634327	2	8	from	properties	522:531	arg1	microstructure					497:510	the microstructure	493:510	the microstructure	493:510	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	0	9	theme	konjac	99:104	arg1	films					157:161	konjac glucomannan/cellulose nanocrystal bionanocomposite films	99:161	konjac glucomannan/cellulose nanocrystal bionanocomposite films	99:161	Investigation of the structural and physical properties, antioxidant and antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films incorporated with phlorotannin from Sargassum.
34634327	3	10	theme	X-ray	676:680	arg1	diffraction					682:692	X-ray diffraction	676:692	X-ray diffraction	676:692	The results of scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectra showed that PS was well compatible with the KGM/CNC composites matrix, which led to form a compact and uniform structure of the films.
34634327	3	11	with	compatible	758:767	arg1	matrix					797:802	the KGM/CNC composites matrix	774:802	the KGM/CNC composites matrix	774:802	The results of scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectra showed that PS was well compatible with the KGM/CNC composites matrix, which led to form a compact and uniform structure of the films.
34634327	6	12	theme	KGM/CNC	1359:1365	arg1	films					1367:1371	the KGM/CNC films	1355:1371	the KGM/CNC films	1355:1371	For instance, with 9% PS, the tensile strength of the KGM/CNC/PS bionanocomposite film increased by 33.9%, and the water-vapor transmittance decreased by 41.67% compared to that of the KGM/CNC films.
34634327	1	13	theme	CNC	399:401	arg1	composites					404:413	konjac glucomannan (KGM)/cotton cellulose nanocrystals (CNC) composites	343:413	konjac glucomannan (KGM)/cotton cellulose nanocrystals (CNC) composites	343:413	In this study, environmentally friendly bionanocomposite films were prepared by incorporating phlorotannins from Sargassum (PS) into konjac glucomannan (KGM)/cotton cellulose nanocrystals (CNC) composites.
34634327	2	14	theme	antibacterial	550:562	arg1	activities					564:573	antibacterial activities	550:573	antibacterial activities of the resultant bionanocomposite films	550:613	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	4	15	theme	scanning	913:920	arg1	calorimetry					922:932	differential scanning calorimetry	900:932	differential scanning calorimetry	900:932	Thermogravimetric analysis and differential scanning calorimetry demonstrated that incorporating PS improved the heat stability of KGM/CNC bionanocomposite films.
34634327	8	16	from	packaging	1578:1586	arg1	industry					1610:1617	the food packaging industry	1591:1617	the food packaging industry	1591:1617	Therefore, KGM/CNC/PS bionanocomposite films have great potential to be applicated as active packaging in the food packaging industry.
34634327	2	17	from	antioxidant	534:544	arg1	microstructure					497:510	the microstructure	493:510	the microstructure	493:510	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	0	18	theme	nanocrystal	128:138	arg1	films					157:161	konjac glucomannan/cellulose nanocrystal bionanocomposite films	99:161	konjac glucomannan/cellulose nanocrystal bionanocomposite films	99:161	Investigation of the structural and physical properties, antioxidant and antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films incorporated with phlorotannin from Sargassum.
34634327	3	19	theme	spectra	726:732	arg1	results					635:641	The results	631:641	The results of scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectra	631:732	The results of scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectra showed that PS was well compatible with the KGM/CNC composites matrix, which led to form a compact and uniform structure of the films.
34634327	2	20	theme	PS	459:460	arg1	concentrations					441:454	different concentrations	431:454	different concentrations of PS (5%, 9%, 13%, and 17%, w/w)	431:488	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	6	21	theme	%	1194:1194	arg1	PS					1196:1197	9% PS	1193:1197	9% PS	1193:1197	For instance, with 9% PS, the tensile strength of the KGM/CNC/PS bionanocomposite film increased by 33.9%, and the water-vapor transmittance decreased by 41.67% compared to that of the KGM/CNC films.
34634327	2	22	theme	different	431:439	arg1	concentrations					441:454	different concentrations	431:454	different concentrations of PS (5%, 9%, 13%, and 17%, w/w)	431:488	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	0	23	theme	glucomannan/cellulose	106:126	arg1	films					157:161	konjac glucomannan/cellulose nanocrystal bionanocomposite films	99:161	konjac glucomannan/cellulose nanocrystal bionanocomposite films	99:161	Investigation of the structural and physical properties, antioxidant and antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films incorporated with phlorotannin from Sargassum.
34634327	7	24	theme	excellent	1433:1441	arg1	properties					1473:1482	excellent antioxidant and antibacterial properties	1433:1482	excellent antioxidant and antibacterial properties	1433:1482	Moreover, the addition of PS endowed the KGM/CNC film with excellent antioxidant and antibacterial properties.
34634327	2	25	theme	films	609:613	arg1	antioxidant					534:544	antioxidant	534:544	antioxidant	534:544	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	2	25	theme	films	609:613	arg1	effects					420:426	The effects	416:426	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure	416:510	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	2	25	theme	films	609:613	arg1	properties					522:531	physical properties	513:531	physical properties	513:531	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	2	25	theme	films	609:613	arg1	activities					564:573	antibacterial activities	550:573	antibacterial activities of the resultant bionanocomposite films	550:613	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	6	26	theme	9	1193:1193	arg1	%					1194:1194	%	1194:1194	%	1194:1194	For instance, with 9% PS, the tensile strength of the KGM/CNC/PS bionanocomposite film increased by 33.9%, and the water-vapor transmittance decreased by 41.67% compared to that of the KGM/CNC films.
34634327	3	27	theme	Fourier-transform	699:715	arg1	spectra					726:732	Fourier-transform infrared spectra	699:732	Fourier-transform infrared spectra	699:732	The results of scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectra showed that PS was well compatible with the KGM/CNC composites matrix, which led to form a compact and uniform structure of the films.
34634327	2	28	theme	bionanocomposite	592:607	arg1	films					609:613	the resultant bionanocomposite films	578:613	the resultant bionanocomposite films	578:613	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	7	29	theme	PS	1400:1401	arg1	addition					1388:1395	the addition	1384:1395	the addition of PS	1384:1401	Moreover, the addition of PS endowed the KGM/CNC film with excellent antioxidant and antibacterial properties.
34634327	5	30	theme	barrier-related	1117:1131	arg1	properties					1133:1142	the mechanical and water-vapor barrier-related properties	1086:1142	the mechanical and water-vapor barrier-related properties of the bionanocomposite film	1086:1171	And addition of the appropriate amount of PS improved the mechanical and water-vapor barrier-related properties of the bionanocomposite film.
34634327	5	31	theme	water-vapor	1105:1115	arg1	properties					1133:1142	the mechanical and water-vapor barrier-related properties	1086:1142	the mechanical and water-vapor barrier-related properties of the bionanocomposite film	1086:1171	And addition of the appropriate amount of PS improved the mechanical and water-vapor barrier-related properties of the bionanocomposite film.
34634327	5	32	theme	film	1168:1171	arg1	properties					1133:1142	the mechanical and water-vapor barrier-related properties	1086:1142	the mechanical and water-vapor barrier-related properties of the bionanocomposite film	1086:1171	And addition of the appropriate amount of PS improved the mechanical and water-vapor barrier-related properties of the bionanocomposite film.
34634327	2	33	from	activities	564:573	arg1	microstructure					497:510	the microstructure	493:510	the microstructure	493:510	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	2	34	theme	resultant	582:590	arg1	films					609:613	the resultant bionanocomposite films	578:613	the resultant bionanocomposite films	578:613	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	5	35	theme	appropriate	1052:1062	arg1	PS					1074:1075	PS	1074:1075	PS	1074:1075	And addition of the appropriate amount of PS improved the mechanical and water-vapor barrier-related properties of the bionanocomposite film.
34634327	5	35	theme	appropriate	1052:1062	arg1	amount					1064:1069	the appropriate amount	1048:1069	the appropriate amount of PS	1048:1075	And addition of the appropriate amount of PS improved the mechanical and water-vapor barrier-related properties of the bionanocomposite film.
34634327	0	36	theme	properties	45:54	arg1	Investigation					0:12	Investigation	0:12	Investigation of the structural and physical properties, antioxidant and antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films	0:161	Investigation of the structural and physical properties, antioxidant and antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films incorporated with phlorotannin from Sargassum.
34634327	8	37	theme	great	1535:1539	arg1	potential					1541:1549	great potential	1535:1549	great potential to be applicated as active packaging in the food packaging industry	1535:1617	Therefore, KGM/CNC/PS bionanocomposite films have great potential to be applicated as active packaging in the food packaging industry.
34634327	0	38	theme	structural	21:30	arg1	antioxidant					57:67	antioxidant	57:67	antioxidant	57:67	Investigation of the structural and physical properties, antioxidant and antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films incorporated with phlorotannin from Sargassum.
34634327	0	38	theme	structural	21:30	arg1	activity					87:94	antimicrobial activity	73:94	antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films	73:161	Investigation of the structural and physical properties, antioxidant and antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films incorporated with phlorotannin from Sargassum.
34634327	0	38	theme	structural	21:30	arg1	properties					45:54	the structural and physical properties	17:54	the structural and physical properties	17:54	Investigation of the structural and physical properties, antioxidant and antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films incorporated with phlorotannin from Sargassum.
34634327	1	39	from	Sargassum	323:331	arg1	phlorotannins					304:316	phlorotannins	304:316	phlorotannins from Sargassum (PS)	304:336	In this study, environmentally friendly bionanocomposite films were prepared by incorporating phlorotannins from Sargassum (PS) into konjac glucomannan (KGM)/cotton cellulose nanocrystals (CNC) composites.
34634327	6	40	theme	film	1256:1259	arg1	strength					1212:1219	the tensile strength	1200:1219	the tensile strength of the KGM/CNC/PS bionanocomposite film	1200:1259	For instance, with 9% PS, the tensile strength of the KGM/CNC/PS bionanocomposite film increased by 33.9%, and the water-vapor transmittance decreased by 41.67% compared to that of the KGM/CNC films.
34634327	1	41	theme	environmentally	225:239	arg1	films					267:271	environmentally friendly bionanocomposite films	225:271	environmentally friendly bionanocomposite films	225:271	In this study, environmentally friendly bionanocomposite films were prepared by incorporating phlorotannins from Sargassum (PS) into konjac glucomannan (KGM)/cotton cellulose nanocrystals (CNC) composites.
34634327	7	42	with	endowed	1403:1409	arg1	properties					1473:1482	excellent antioxidant and antibacterial properties	1433:1482	excellent antioxidant and antibacterial properties	1433:1482	Moreover, the addition of PS endowed the KGM/CNC film with excellent antioxidant and antibacterial properties.
34634327	5	43	theme	bionanocomposite	1151:1166	arg1	film					1168:1171	the bionanocomposite film	1147:1171	the bionanocomposite film	1147:1171	And addition of the appropriate amount of PS improved the mechanical and water-vapor barrier-related properties of the bionanocomposite film.
34634327	0	44	theme	bionanocomposite	140:155	arg1	films					157:161	konjac glucomannan/cellulose nanocrystal bionanocomposite films	99:161	konjac glucomannan/cellulose nanocrystal bionanocomposite films	99:161	Investigation of the structural and physical properties, antioxidant and antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films incorporated with phlorotannin from Sargassum.
34634327	1	45	theme	friendly	241:248	arg1	films					267:271	environmentally friendly bionanocomposite films	225:271	environmentally friendly bionanocomposite films	225:271	In this study, environmentally friendly bionanocomposite films were prepared by incorporating phlorotannins from Sargassum (PS) into konjac glucomannan (KGM)/cotton cellulose nanocrystals (CNC) composites.
34634327	0	46	theme	physical	36:43	arg1	antioxidant					57:67	antioxidant	57:67	antioxidant	57:67	Investigation of the structural and physical properties, antioxidant and antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films incorporated with phlorotannin from Sargassum.
34634327	0	46	theme	physical	36:43	arg1	activity					87:94	antimicrobial activity	73:94	antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films	73:161	Investigation of the structural and physical properties, antioxidant and antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films incorporated with phlorotannin from Sargassum.
34634327	0	46	theme	physical	36:43	arg1	properties					45:54	the structural and physical properties	17:54	the structural and physical properties	17:54	Investigation of the structural and physical properties, antioxidant and antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films incorporated with phlorotannin from Sargassum.
34634327	8	47	theme	active	1571:1576	arg1	packaging					1578:1586	active packaging	1571:1586	active packaging in the food packaging industry	1571:1617	Therefore, KGM/CNC/PS bionanocomposite films have great potential to be applicated as active packaging in the food packaging industry.
34634327	3	48	theme	infrared	717:724	arg1	spectra					726:732	Fourier-transform infrared spectra	699:732	Fourier-transform infrared spectra	699:732	The results of scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectra showed that PS was well compatible with the KGM/CNC composites matrix, which led to form a compact and uniform structure of the films.
34634327	1	49	theme	bionanocomposite	250:265	arg1	films					267:271	environmentally friendly bionanocomposite films	225:271	environmentally friendly bionanocomposite films	225:271	In this study, environmentally friendly bionanocomposite films were prepared by incorporating phlorotannins from Sargassum (PS) into konjac glucomannan (KGM)/cotton cellulose nanocrystals (CNC) composites.
34634327	4	50	theme	KGM/CNC	1000:1006	arg1	films					1025:1029	KGM/CNC bionanocomposite films	1000:1029	KGM/CNC bionanocomposite films	1000:1029	Thermogravimetric analysis and differential scanning calorimetry demonstrated that incorporating PS improved the heat stability of KGM/CNC bionanocomposite films.
34634327	4	51	theme	films	1025:1029	arg1	stability					987:995	the heat stability	978:995	the heat stability of KGM/CNC bionanocomposite films	978:1029	Thermogravimetric analysis and differential scanning calorimetry demonstrated that incorporating PS improved the heat stability of KGM/CNC bionanocomposite films.
34634327	5	52	theme	mechanical	1090:1099	arg1	properties					1133:1142	the mechanical and water-vapor barrier-related properties	1086:1142	the mechanical and water-vapor barrier-related properties of the bionanocomposite film	1086:1171	And addition of the appropriate amount of PS improved the mechanical and water-vapor barrier-related properties of the bionanocomposite film.
34634327	6	53	theme	bionanocomposite	1239:1254	arg1	film					1256:1259	the KGM/CNC/PS bionanocomposite film	1224:1259	the KGM/CNC/PS bionanocomposite film	1224:1259	For instance, with 9% PS, the tensile strength of the KGM/CNC/PS bionanocomposite film increased by 33.9%, and the water-vapor transmittance decreased by 41.67% compared to that of the KGM/CNC films.
34634327	2	54	from	effects	420:426	arg1	microstructure					497:510	the microstructure	493:510	the microstructure	493:510	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	2	55	theme	concentrations	441:454	arg1	antioxidant					534:544	antioxidant	534:544	antioxidant	534:544	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	2	55	theme	concentrations	441:454	arg1	effects					420:426	The effects	416:426	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure	416:510	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	2	55	theme	concentrations	441:454	arg1	properties					522:531	physical properties	513:531	physical properties	513:531	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	2	55	theme	concentrations	441:454	arg1	activities					564:573	antibacterial activities	550:573	antibacterial activities of the resultant bionanocomposite films	550:613	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	4	56	theme	incorporating	952:964	arg1	PS					966:967	incorporating PS	952:967	incorporating PS	952:967	Thermogravimetric analysis and differential scanning calorimetry demonstrated that incorporating PS improved the heat stability of KGM/CNC bionanocomposite films.
34634327	4	57	theme	Thermogravimetric	869:885	arg1	analysis					887:894	Thermogravimetric analysis	869:894	Thermogravimetric analysis	869:894	Thermogravimetric analysis and differential scanning calorimetry demonstrated that incorporating PS improved the heat stability of KGM/CNC bionanocomposite films.
34634327	5	58	theme	amount	1064:1069	arg1	addition					1036:1043	addition	1036:1043	addition of the appropriate amount of PS	1036:1075	And addition of the appropriate amount of PS improved the mechanical and water-vapor barrier-related properties of the bionanocomposite film.
34634327	6	59	theme	KGM/CNC/PS	1228:1237	arg1	film					1256:1259	the KGM/CNC/PS bionanocomposite film	1224:1259	the KGM/CNC/PS bionanocomposite film	1224:1259	For instance, with 9% PS, the tensile strength of the KGM/CNC/PS bionanocomposite film increased by 33.9%, and the water-vapor transmittance decreased by 41.67% compared to that of the KGM/CNC films.
34634327	8	60	theme	bionanocomposite	1507:1522	arg1	films					1524:1528	KGM/CNC/PS bionanocomposite films	1496:1528	KGM/CNC/PS bionanocomposite films	1496:1528	Therefore, KGM/CNC/PS bionanocomposite films have great potential to be applicated as active packaging in the food packaging industry.
34634327	0	61	theme	films	157:161	arg1	antioxidant					57:67	antioxidant	57:67	antioxidant	57:67	Investigation of the structural and physical properties, antioxidant and antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films incorporated with phlorotannin from Sargassum.
34634327	0	61	theme	films	157:161	arg1	activity					87:94	antimicrobial activity	73:94	antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films	73:161	Investigation of the structural and physical properties, antioxidant and antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films incorporated with phlorotannin from Sargassum.
34634327	0	61	theme	films	157:161	arg1	properties					45:54	the structural and physical properties	17:54	the structural and physical properties	17:54	Investigation of the structural and physical properties, antioxidant and antimicrobial activity of konjac glucomannan/cellulose nanocrystal bionanocomposite films incorporated with phlorotannin from Sargassum.
34634327	8	62	theme	packaging	1600:1608	arg1	industry					1610:1617	the food packaging industry	1591:1617	the food packaging industry	1591:1617	Therefore, KGM/CNC/PS bionanocomposite films have great potential to be applicated as active packaging in the food packaging industry.
34634327	2	63	theme	physical	513:520	arg1	properties					522:531	physical properties	513:531	physical properties	513:531	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	3	64	theme	electron	655:662	arg1	microscopy					664:673	scanning electron microscopy	646:673	scanning electron microscopy	646:673	The results of scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectra showed that PS was well compatible with the KGM/CNC composites matrix, which led to form a compact and uniform structure of the films.
34634327	2	65	dep	PS	459:460	arg1	w/w					485:487	w/w	485:487	w/w	485:487	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	2	65	dep	PS	459:460	arg1	%					482:482	17%	480:482	17%	480:482	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	2	65	dep	PS	459:460	arg1	%					464:464	5%	463:464	5%	463:464	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	2	65	dep	PS	459:460	arg1	%					468:468	9%	467:468	9%	467:468	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	2	65	dep	PS	459:460	arg1	%					473:473	13%	471:473	13%	471:473	The effects of different concentrations of PS (5%, 9%, 13%, and 17%, w/w) on the microstructure, physical properties, antioxidant and antibacterial activities of the resultant bionanocomposite films were evaluated.
34634327	3	66	theme	diffraction	682:692	arg1	results					635:641	The results	631:641	The results of scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectra	631:732	The results of scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectra showed that PS was well compatible with the KGM/CNC composites matrix, which led to form a compact and uniform structure of the films.
34634327	8	67	theme	food	1595:1598	arg1	industry					1610:1617	the food packaging industry	1591:1617	the food packaging industry	1591:1617	Therefore, KGM/CNC/PS bionanocomposite films have great potential to be applicated as active packaging in the food packaging industry.
34634327	3	68	theme	microscopy	664:673	arg1	results					635:641	The results	631:641	The results of scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectra	631:732	The results of scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectra showed that PS was well compatible with the KGM/CNC composites matrix, which led to form a compact and uniform structure of the films.
34634327	7	69	theme	antibacterial	1459:1471	arg1	properties					1473:1482	excellent antioxidant and antibacterial properties	1433:1482	excellent antioxidant and antibacterial properties	1433:1482	Moreover, the addition of PS endowed the KGM/CNC film with excellent antioxidant and antibacterial properties.
34634327	3	70	theme	uniform	837:843	arg1	structure					845:853	a compact and uniform structure	823:853	a compact and uniform structure of the films	823:866	The results of scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectra showed that PS was well compatible with the KGM/CNC composites matrix, which led to form a compact and uniform structure of the films.
34634327	7	71	theme	KGM/CNC	1415:1421	arg1	film					1423:1426	the KGM/CNC film	1411:1426	the KGM/CNC film	1411:1426	Moreover, the addition of PS endowed the KGM/CNC film with excellent antioxidant and antibacterial properties.
34634327	8	72	theme	KGM/CNC/PS	1496:1505	arg1	films					1524:1528	KGM/CNC/PS bionanocomposite films	1496:1528	KGM/CNC/PS bionanocomposite films	1496:1528	Therefore, KGM/CNC/PS bionanocomposite films have great potential to be applicated as active packaging in the food packaging industry.
34634327	8	73	contain	have	1530:1533	arg1	films					1524:1528	KGM/CNC/PS bionanocomposite films	1496:1528	KGM/CNC/PS bionanocomposite films	1496:1528	Therefore, KGM/CNC/PS bionanocomposite films have great potential to be applicated as active packaging in the food packaging industry.
34634327	8	73	contain	have	1530:1533	arg2	potential					1541:1549	great potential	1535:1549	great potential to be applicated as active packaging in the food packaging industry	1535:1617	Therefore, KGM/CNC/PS bionanocomposite films have great potential to be applicated as active packaging in the food packaging industry.
34634327	4	74	theme	differential	900:911	arg1	calorimetry					922:932	differential scanning calorimetry	900:932	differential scanning calorimetry	900:932	Thermogravimetric analysis and differential scanning calorimetry demonstrated that incorporating PS improved the heat stability of KGM/CNC bionanocomposite films.
34634327	4	75	theme	heat	982:985	arg1	stability					987:995	the heat stability	978:995	the heat stability of KGM/CNC bionanocomposite films	978:1029	Thermogravimetric analysis and differential scanning calorimetry demonstrated that incorporating PS improved the heat stability of KGM/CNC bionanocomposite films.
34634327	3	76	theme	scanning	646:653	arg1	microscopy					664:673	scanning electron microscopy	646:673	scanning electron microscopy	646:673	The results of scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectra showed that PS was well compatible with the KGM/CNC composites matrix, which led to form a compact and uniform structure of the films.
34634327	6	77	theme	tensile	1204:1210	arg1	strength					1212:1219	the tensile strength	1200:1219	the tensile strength of the KGM/CNC/PS bionanocomposite film	1200:1259	For instance, with 9% PS, the tensile strength of the KGM/CNC/PS bionanocomposite film increased by 33.9%, and the water-vapor transmittance decreased by 41.67% compared to that of the KGM/CNC films.
34634327	3	78	theme	compact	825:831	arg1	structure					845:853	a compact and uniform structure	823:853	a compact and uniform structure of the films	823:866	The results of scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectra showed that PS was well compatible with the KGM/CNC composites matrix, which led to form a compact and uniform structure of the films.
34634327	3	79	theme	KGM/CNC	778:784	arg1	matrix					797:802	the KGM/CNC composites matrix	774:802	the KGM/CNC composites matrix	774:802	The results of scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectra showed that PS was well compatible with the KGM/CNC composites matrix, which led to form a compact and uniform structure of the films.
34634327	1	80	theme	/cotton	367:373	arg1	composites					404:413	konjac glucomannan (KGM)/cotton cellulose nanocrystals (CNC) composites	343:413	konjac glucomannan (KGM)/cotton cellulose nanocrystals (CNC) composites	343:413	In this study, environmentally friendly bionanocomposite films were prepared by incorporating phlorotannins from Sargassum (PS) into konjac glucomannan (KGM)/cotton cellulose nanocrystals (CNC) composites.
34634327	3	81	theme	composites	786:795	arg1	matrix					797:802	the KGM/CNC composites matrix	774:802	the KGM/CNC composites matrix	774:802	The results of scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectra showed that PS was well compatible with the KGM/CNC composites matrix, which led to form a compact and uniform structure of the films.
34142506	14	0	theme	mass	2079:2082	arg1	P<0.05					2093:2098	P<0.05	2093:2098	P<0.05	2093:2098	All conduits had different degrees of mass loss, and the higher the degree of acetylation, the greater the mass change ( P<0.05).
34142506	14	0	theme	mass	2079:2082	arg1	change					2084:2089	the mass change	2075:2089	the greater the mass change ( P<0.05)	2063:2099	All conduits had different degrees of mass loss, and the higher the degree of acetylation, the greater the mass change ( P<0.05).
34142506	18	1	theme	different	2645:2653	arg1	degrees					2667:2673	different acetylation degrees	2645:2673	different acetylation degrees	2645:2673	CONCLUSION The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees has good biocompatibility, conductivity, and biodegradability correlated with acetylation degree in vivo, which provide a new scaffold material for the construction of tissue engineered nerve.
34142506	8	2	located	observed	1251:1258	arg1	weeks					1162:1166	2, 4, 6, 8, 10, and 12 weeks	1139:1166	2, 4, 6, 8, 10, and 12 weeks after operation	1139:1182	At 2, 4, 6, 8, 10, and 12 weeks after operation, the morphology, the microstructure, and the degradation rate were observed and measured to assess the in vivo degradation of conduits.
34142506	8	2	located	observed	1251:1258	arg2	microstructure					1205:1218	the microstructure	1201:1218	the microstructure	1201:1218	At 2, 4, 6, 8, 10, and 12 weeks after operation, the morphology, the microstructure, and the degradation rate were observed and measured to assess the in vivo degradation of conduits.
34142506	8	2	located	observed	1251:1258	arg2	rate					1241:1244	the degradation rate	1225:1244	the degradation rate	1225:1244	At 2, 4, 6, 8, 10, and 12 weeks after operation, the morphology, the microstructure, and the degradation rate were observed and measured to assess the in vivo degradation of conduits.
34142506	8	2	located	observed	1251:1258	arg2	morphology					1189:1198	the morphology	1185:1198	the morphology	1185:1198	At 2, 4, 6, 8, 10, and 12 weeks after operation, the morphology, the microstructure, and the degradation rate were observed and measured to assess the in vivo degradation of conduits.
34142506	10	3	theme	Ⅱ	1484:1484	arg1	band					1486:1489	the amide Ⅱ band	1474:1489	the amide Ⅱ band	1474:1489	RESULTS The characteristic peaks of the amide Ⅱ band around 1 562 cm -1 appeared after being acetylated, indicating that the acetylation modification of chitosan was successful.
34142506	15	4	theme	more	2141:2144	arg1	pores					2146:2150	more pores	2141:2150	more pores	2141:2150	SEM observation showed that there were more pores at 12 weeks after implantation, and the pores showed an increasing trend as the degree of acetylation increased.
34142506	18	5	theme	good	2679:2682	arg1	biocompatibility					2684:2699	good biocompatibility	2679:2699	good biocompatibility	2679:2699	CONCLUSION The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees has good biocompatibility, conductivity, and biodegradability correlated with acetylation degree in vivo, which provide a new scaffold material for the construction of tissue engineered nerve.
34142506	5	6	theme	absorption	821:830	arg1	spectrum					832:839	Fourier infrared absorption spectrum	804:839	Fourier infrared absorption spectrum	804:839	Fourier infrared absorption spectrum and scanning electron microscopy (SEM) were used to identify the conduits.
34142506	16	7	theme	macrophages	2318:2328	arg1	infiltration					2346:2357	more macrophages and lymphocytes infiltration	2313:2357	more macrophages and lymphocytes infiltration	2313:2357	Histological observation showed that there were more macrophages and lymphocytes infiltration in each group at the early stage.
34142506	4	8	theme	CP	662:663	arg1	conduits					665:672	the CP conduits	658:672	the CP conduits with different acetyl degree	658:701	Then the CP conduits with different acetyl degree were resulted undergoing varying acetylation for 30, 60, and 90 minutes (CAP1, CAP2, CAP3 conduits).
34142506	1	9	theme	chitosan	195:202	arg1	histocompatibility					164:181	histocompatibility	164:181	histocompatibility	164:181	OBJECTIVE To investigate the in vivo degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit, so as to provide a new scaffold material for the construction of tissue engineered nerve.
34142506	1	9	theme	chitosan	195:202	arg1	degradation					148:158	in vivo degradation	140:158	in vivo degradation	140:158	OBJECTIVE To investigate the in vivo degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit, so as to provide a new scaffold material for the construction of tissue engineered nerve.
34142506	17	10	theme	collagen	2484:2491	arg1	fibers					2493:2498	collagen fibers	2484:2498	collagen fibers	2484:2498	With the extension of implantation time, lymphocytes decreased, fibroblasts increased, and collagen fibers proliferated significantly.
34142506	15	11	theme	increasing	2208:2217	arg1	trend					2219:2223	an increasing trend	2205:2223	an increasing trend	2205:2223	SEM observation showed that there were more pores at 12 weeks after implantation, and the pores showed an increasing trend as the degree of acetylation increased.
34142506	12	12	from	groups	1760:1765	arg1	similar					1772:1778	similar	1772:1778	similar	1772:1778	SEM observation showed that the surfaces of the conduits in all groups were similar with relatively smooth surface and compact structure.
34142506	12	12	from	groups	1760:1765	arg1	surfaces					1728:1735	the surfaces	1724:1735	the surfaces of the conduits in all groups	1724:1765	SEM observation showed that the surfaces of the conduits in all groups were similar with relatively smooth surface and compact structure.
34142506	2	13	theme	formation	528:536	arg1	model					538:542	a customized conduit formation model	507:542	a customized conduit formation model	507:542	METHODS The nano polypyrrole (PPy) was synthesized by microemulsion polymerization, blended with chitosan, and then formed conduit by injecting the mixed solution into a customized conduit formation model.
34142506	7	14	theme	subcutaneous	1023:1034	arg1	tunnels					1044:1050	the subcutaneous fascial tunnels	1019:1050	the subcutaneous fascial tunnels	1019:1050	The above conduits were implanted after the subcutaneous fascial tunnels were made symmetrically on both sides of the back of 30 female Sprague Dawley rats.
34142506	18	15	theme	scaffold	2801:2808	arg1	material					2810:2817	a new scaffold material	2795:2817	a new scaffold material for the construction of tissue engineered nerve	2795:2865	CONCLUSION The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees has good biocompatibility, conductivity, and biodegradability correlated with acetylation degree in vivo, which provide a new scaffold material for the construction of tissue engineered nerve.
34142506	3	16	theme	PPy/chitosan	595:606	arg1	conduit					618:624	the nano PPy/chitosan composite conduit	586:624	the nano PPy/chitosan composite conduit (CP conduit)	586:637	After freeze-drying and deacidification, the nano PPy/chitosan composite conduit (CP conduit) was prepared.
34142506	3	16	theme	PPy/chitosan	595:606	arg1	conduit					630:636	CP conduit	627:636	CP conduit	627:636	After freeze-drying and deacidification, the nano PPy/chitosan composite conduit (CP conduit) was prepared.
34142506	1	17	theme	composite	224:232	arg1	conduit					240:246	conductive composite nerve conduit	213:246	conductive composite nerve conduit	213:246	OBJECTIVE To investigate the in vivo degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit, so as to provide a new scaffold material for the construction of tissue engineered nerve.
34142506	11	18	from	difference	1641:1650	arg1	conductivity					1655:1666	conductivity	1655:1666	conductivity	1655:1666	There was no significant difference in conductivity between conduits ( P>0.05).
34142506	14	19	theme	loss	2015:2018	arg1	degrees					1999:2005	different degrees	1989:2005	different degrees of mass loss	1989:2018	All conduits had different degrees of mass loss, and the higher the degree of acetylation, the greater the mass change ( P<0.05).
34142506	18	20	theme	tissue	2843:2848	arg1	construction					2827:2838	the construction	2823:2838	the construction of tissue engineered nerve	2823:2865	CONCLUSION The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees has good biocompatibility, conductivity, and biodegradability correlated with acetylation degree in vivo, which provide a new scaffold material for the construction of tissue engineered nerve.
34142506	17	21	theme	time	2428:2431	arg1	extension					2402:2410	the extension	2398:2410	the extension of implantation time	2398:2431	With the extension of implantation time, lymphocytes decreased, fibroblasts increased, and collagen fibers proliferated significantly.
34142506	2	22	dep	METHODS	339:345	arg1	PPy					369:371	PPy	369:371	PPy	369:371	METHODS The nano polypyrrole (PPy) was synthesized by microemulsion polymerization, blended with chitosan, and then formed conduit by injecting the mixed solution into a customized conduit formation model.
34142506	2	22	dep	METHODS	339:345	arg1	polypyrrole					356:366	The nano polypyrrole	347:366	METHODS The nano polypyrrole (PPy)	339:372	METHODS The nano polypyrrole (PPy) was synthesized by microemulsion polymerization, blended with chitosan, and then formed conduit by injecting the mixed solution into a customized conduit formation model.
34142506	3	23	theme	CP	627:628	arg1	conduit					618:624	the nano PPy/chitosan composite conduit	586:624	the nano PPy/chitosan composite conduit (CP conduit)	586:637	After freeze-drying and deacidification, the nano PPy/chitosan composite conduit (CP conduit) was prepared.
34142506	3	23	theme	CP	627:628	arg1	conduit					630:636	CP conduit	627:636	CP conduit	627:636	After freeze-drying and deacidification, the nano PPy/chitosan composite conduit (CP conduit) was prepared.
34142506	2	24	theme	mixed	487:491	arg1	solution					493:500	the mixed solution	483:500	the mixed solution	483:500	METHODS The nano polypyrrole (PPy) was synthesized by microemulsion polymerization, blended with chitosan, and then formed conduit by injecting the mixed solution into a customized conduit formation model.
34142506	18	25	theme	chitosan	2552:2559	arg1	conduit					2596:2602	The modified chitosan basedon conductive composite nerve conduit	2539:2602	The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees	2539:2673	CONCLUSION The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees has good biocompatibility, conductivity, and biodegradability correlated with acetylation degree in vivo, which provide a new scaffold material for the construction of tissue engineered nerve.
34142506	8	26	dep	in	1287:1288	arg1	vivo					1290:1293	vivo	1290:1293	vivo	1290:1293	At 2, 4, 6, 8, 10, and 12 weeks after operation, the morphology, the microstructure, and the degradation rate were observed and measured to assess the in vivo degradation of conduits.
34142506	14	27	dep	greater	2067:2073	arg1	P<0.05					2093:2098	P<0.05	2093:2098	P<0.05	2093:2098	All conduits had different degrees of mass loss, and the higher the degree of acetylation, the greater the mass change ( P<0.05).
34142506	14	27	dep	greater	2067:2073	arg1	change					2084:2089	the mass change	2075:2089	the greater the mass change ( P<0.05)	2063:2099	All conduits had different degrees of mass loss, and the higher the degree of acetylation, the greater the mass change ( P<0.05).
34142506	14	28	theme	mass	2010:2013	arg1	loss					2015:2018	mass loss	2010:2018	mass loss	2010:2018	All conduits had different degrees of mass loss, and the higher the degree of acetylation, the greater the mass change ( P<0.05).
34142506	4	29	theme	acetyl	689:694	arg1	degree					696:701	different acetyl degree	679:701	different acetyl degree	679:701	Then the CP conduits with different acetyl degree were resulted undergoing varying acetylation for 30, 60, and 90 minutes (CAP1, CAP2, CAP3 conduits).
34142506	0	30	theme	conductive	74:83	arg1	conduit					101:107	conductive composite nerve conduit	74:107	conductive composite nerve conduit	74:107	[In vivo degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit].
34142506	13	31	theme	CAP3	1958:1961	arg1	conduit					1963:1969	the CAP3 conduit	1954:1969	the CAP3 conduit	1954:1969	After the conduits were implanted into the rats, with the extension of time, all conduits were collapsed, especially on the CAP3 conduit.
34142506	12	32	theme	compact	1815:1821	arg1	structure					1823:1831	compact structure	1815:1831	compact structure	1815:1831	SEM observation showed that the surfaces of the conduits in all groups were similar with relatively smooth surface and compact structure.
34142506	2	33	theme	customized	509:518	arg1	model					538:542	a customized conduit formation model	507:542	a customized conduit formation model	507:542	METHODS The nano polypyrrole (PPy) was synthesized by microemulsion polymerization, blended with chitosan, and then formed conduit by injecting the mixed solution into a customized conduit formation model.
34142506	8	34	theme	degradation	1229:1239	arg1	rate					1241:1244	the degradation rate	1225:1244	the degradation rate	1225:1244	At 2, 4, 6, 8, 10, and 12 weeks after operation, the morphology, the microstructure, and the degradation rate were observed and measured to assess the in vivo degradation of conduits.
34142506	18	35	theme	nerve	2590:2594	arg1	conduit					2596:2602	The modified chitosan basedon conductive composite nerve conduit	2539:2602	The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees	2539:2673	CONCLUSION The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees has good biocompatibility, conductivity, and biodegradability correlated with acetylation degree in vivo, which provide a new scaffold material for the construction of tissue engineered nerve.
34142506	0	36	theme	nerve	95:99	arg1	conduit					101:107	conductive composite nerve conduit	74:107	conductive composite nerve conduit	74:107	[In vivo degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit].
34142506	1	37	dep	degradation	148:158	arg1	the					136:138	the	136:138	the	136:138	OBJECTIVE To investigate the in vivo degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit, so as to provide a new scaffold material for the construction of tissue engineered nerve.
34142506	0	38	theme	[In	0:2	arg1	degradation					9:19	[In vivo degradation	0:19	[In vivo degradation	0:19	[In vivo degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit].
34142506	1	39	theme	new	268:270	arg1	material					281:288	a new scaffold material	266:288	a new scaffold material for the construction of tissue engineered nerve	266:336	OBJECTIVE To investigate the in vivo degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit, so as to provide a new scaffold material for the construction of tissue engineered nerve.
34142506	14	40	contain	had	1985:1987	arg1	conduits					1976:1983	All conduits	1972:1983	All conduits	1972:1983	All conduits had different degrees of mass loss, and the higher the degree of acetylation, the greater the mass change ( P<0.05).
34142506	14	40	contain	had	1985:1987	arg2	degrees					1999:2005	different degrees	1989:2005	different degrees of mass loss	1989:2018	All conduits had different degrees of mass loss, and the higher the degree of acetylation, the greater the mass change ( P<0.05).
34142506	14	40	contain	had	1985:1987	arg2	higher					2029:2034	higher	2029:2034	higher	2029:2034	All conduits had different degrees of mass loss, and the higher the degree of acetylation, the greater the mass change ( P<0.05).
34142506	1	41	dep	in	140:141	arg1	vivo					143:146	vivo	143:146	vivo	143:146	OBJECTIVE To investigate the in vivo degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit, so as to provide a new scaffold material for the construction of tissue engineered nerve.
34142506	7	42	theme	Dawley	1123:1128	arg1	rats					1130:1133	30 female Sprague Dawley rats	1105:1133	30 female Sprague Dawley rats	1105:1133	The above conduits were implanted after the subcutaneous fascial tunnels were made symmetrically on both sides of the back of 30 female Sprague Dawley rats.
34142506	1	43	theme	in	140:141	arg1	degradation					148:158	in vivo degradation	140:158	in vivo degradation	140:158	OBJECTIVE To investigate the in vivo degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit, so as to provide a new scaffold material for the construction of tissue engineered nerve.
34142506	2	44	theme	microemulsion	393:405	arg1	polymerization					407:420	microemulsion polymerization	393:420	microemulsion polymerization	393:420	METHODS The nano polypyrrole (PPy) was synthesized by microemulsion polymerization, blended with chitosan, and then formed conduit by injecting the mixed solution into a customized conduit formation model.
34142506	10	45	theme	acetylation	1563:1573	arg1	successful					1604:1613	successful	1604:1613	successful	1604:1613	RESULTS The characteristic peaks of the amide Ⅱ band around 1 562 cm -1 appeared after being acetylated, indicating that the acetylation modification of chitosan was successful.
34142506	10	45	theme	acetylation	1563:1573	arg1	modification					1575:1586	the acetylation modification	1559:1586	the acetylation modification of chitosan	1559:1598	RESULTS The characteristic peaks of the amide Ⅱ band around 1 562 cm -1 appeared after being acetylated, indicating that the acetylation modification of chitosan was successful.
34142506	9	46	theme	HE	1320:1321	arg1	staining					1323:1330	HE staining	1320:1330	HE staining	1320:1330	HE staining and anti-macrophage immunofluorescence staining were performed to observe the histocompatibility in vivo.
34142506	7	47	theme	female	1108:1113	arg1	rats					1130:1133	30 female Sprague Dawley rats	1105:1133	30 female Sprague Dawley rats	1105:1133	The above conduits were implanted after the subcutaneous fascial tunnels were made symmetrically on both sides of the back of 30 female Sprague Dawley rats.
34142506	18	48	dep	CONCLUSION	2528:2537	arg1	has					2675:2677	has	2675:2677	has good biocompatibility, conductivity, and biodegradability correlated with acetylation degree in vivo, which provide a new scaffold material for the construction of tissue engineered nerve	2675:2865	CONCLUSION The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees has good biocompatibility, conductivity, and biodegradability correlated with acetylation degree in vivo, which provide a new scaffold material for the construction of tissue engineered nerve.
34142506	4	49	theme	CAP3	788:791	arg1	conduits					793:800	CAP3 conduits	788:800	CAP3 conduits	788:800	Then the CP conduits with different acetyl degree were resulted undergoing varying acetylation for 30, 60, and 90 minutes (CAP1, CAP2, CAP3 conduits).
34142506	4	49	theme	CAP3	788:791	arg1	CAP1					776:779	CAP1	776:779	CAP1	776:779	Then the CP conduits with different acetyl degree were resulted undergoing varying acetylation for 30, 60, and 90 minutes (CAP1, CAP2, CAP3 conduits).
34142506	0	50	theme	modified	47:54	arg1	chitosan					56:63	modified chitosan	47:63	modified chitosan	47:63	[In vivo degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit].
34142506	12	51	from	surfaces	1728:1735	arg1	groups					1760:1765	all groups	1756:1765	all groups	1756:1765	SEM observation showed that the surfaces of the conduits in all groups were similar with relatively smooth surface and compact structure.
34142506	11	52	theme	significant	1629:1639	arg1	difference					1641:1650	no significant difference	1626:1650	no significant difference in conductivity between conduits ( P>0.05)	1626:1693	There was no significant difference in conductivity between conduits ( P>0.05).
34142506	1	53	theme	nerve	234:238	arg1	conduit					240:246	conductive composite nerve conduit	213:246	conductive composite nerve conduit	213:246	OBJECTIVE To investigate the in vivo degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit, so as to provide a new scaffold material for the construction of tissue engineered nerve.
34142506	16	54	theme	early	2380:2384	arg1	stage					2386:2390	the early stage	2376:2390	the early stage	2376:2390	Histological observation showed that there were more macrophages and lymphocytes infiltration in each group at the early stage.
34142506	12	55	theme	SEM	1696:1698	arg1	observation					1700:1710	SEM observation	1696:1710	SEM observation	1696:1710	SEM observation showed that the surfaces of the conduits in all groups were similar with relatively smooth surface and compact structure.
34142506	5	56	theme	electron	854:861	arg1	microscopy					863:872	scanning electron microscopy	845:872	scanning electron microscopy (SEM)	845:878	Fourier infrared absorption spectrum and scanning electron microscopy (SEM) were used to identify the conduits.
34142506	5	56	theme	electron	854:861	arg1	SEM					875:877	SEM	875:877	SEM	875:877	Fourier infrared absorption spectrum and scanning electron microscopy (SEM) were used to identify the conduits.
34142506	7	57	theme	back	1097:1100	arg1	sides					1084:1088	both sides	1079:1088	both sides of the back of 30 female Sprague Dawley rats	1079:1133	The above conduits were implanted after the subcutaneous fascial tunnels were made symmetrically on both sides of the back of 30 female Sprague Dawley rats.
34142506	0	58	dep	[In	0:2	arg1	vivo					4:7	vivo	4:7	vivo	4:7	[In vivo degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit].
34142506	10	59	theme	characteristic	1450:1463	arg1	peaks					1465:1469	The characteristic peaks	1446:1469	The characteristic peaks of the amide Ⅱ band around 1 562 cm -1	1446:1508	RESULTS The characteristic peaks of the amide Ⅱ band around 1 562 cm -1 appeared after being acetylated, indicating that the acetylation modification of chitosan was successful.
34142506	10	60	dep	RESULTS	1438:1444	arg1	appeared					1510:1517	appeared	1510:1517	appeared	1510:1517	RESULTS The characteristic peaks of the amide Ⅱ band around 1 562 cm -1 appeared after being acetylated, indicating that the acetylation modification of chitosan was successful.
34142506	2	61	theme	nano	351:354	arg1	PPy					369:371	PPy	369:371	PPy	369:371	METHODS The nano polypyrrole (PPy) was synthesized by microemulsion polymerization, blended with chitosan, and then formed conduit by injecting the mixed solution into a customized conduit formation model.
34142506	2	61	theme	nano	351:354	arg1	polypyrrole					356:366	The nano polypyrrole	347:366	METHODS The nano polypyrrole (PPy)	339:372	METHODS The nano polypyrrole (PPy) was synthesized by microemulsion polymerization, blended with chitosan, and then formed conduit by injecting the mixed solution into a customized conduit formation model.
34142506	10	62	theme	band	1486:1489	arg1	peaks					1465:1469	The characteristic peaks	1446:1469	The characteristic peaks of the amide Ⅱ band around 1 562 cm -1	1446:1508	RESULTS The characteristic peaks of the amide Ⅱ band around 1 562 cm -1 appeared after being acetylated, indicating that the acetylation modification of chitosan was successful.
34142506	16	63	theme	more	2313:2316	arg1	infiltration					2346:2357	more macrophages and lymphocytes infiltration	2313:2357	more macrophages and lymphocytes infiltration	2313:2357	Histological observation showed that there were more macrophages and lymphocytes infiltration in each group at the early stage.
34142506	10	64	mod	modification	1575:1586	arg1	chitosan					1591:1598	chitosan	1591:1598	chitosan	1591:1598	RESULTS The characteristic peaks of the amide Ⅱ band around 1 562 cm -1 appeared after being acetylated, indicating that the acetylation modification of chitosan was successful.
34142506	10	64	mod	modification	1575:1586	arg3	acetylation					1563:1573	the acetylation modification	1559:1586	the acetylation modification of chitosan	1559:1598	RESULTS The characteristic peaks of the amide Ⅱ band around 1 562 cm -1 appeared after being acetylated, indicating that the acetylation modification of chitosan was successful.
34142506	10	65	theme	amide	1478:1482	arg1	band					1486:1489	the amide Ⅱ band	1474:1489	the amide Ⅱ band	1474:1489	RESULTS The characteristic peaks of the amide Ⅱ band around 1 562 cm -1 appeared after being acetylated, indicating that the acetylation modification of chitosan was successful.
34142506	18	66	theme	nano-PPy/chitosan	2612:2628	arg1	composite					2630:2638	nano-PPy/chitosan composite	2612:2638	nano-PPy/chitosan composite	2612:2638	CONCLUSION The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees has good biocompatibility, conductivity, and biodegradability correlated with acetylation degree in vivo, which provide a new scaffold material for the construction of tissue engineered nerve.
34142506	1	67	theme	modified	186:193	arg1	chitosan					195:202	modified chitosan	186:202	modified chitosan based on conductive composite nerve conduit, so as to provide a new scaffold material for the construction of tissue engineered nerve	186:336	OBJECTIVE To investigate the in vivo degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit, so as to provide a new scaffold material for the construction of tissue engineered nerve.
34142506	5	68	theme	infrared	812:819	arg1	spectrum					832:839	Fourier infrared absorption spectrum	804:839	Fourier infrared absorption spectrum	804:839	Fourier infrared absorption spectrum and scanning electron microscopy (SEM) were used to identify the conduits.
34142506	4	69	with	conduits	665:672	arg1	degree					696:701	different acetyl degree	679:701	different acetyl degree	679:701	Then the CP conduits with different acetyl degree were resulted undergoing varying acetylation for 30, 60, and 90 minutes (CAP1, CAP2, CAP3 conduits).
34142506	4	70	dep	minutes	767:773	arg1	CAP2					782:785	CAP2	782:785	CAP2	782:785	Then the CP conduits with different acetyl degree were resulted undergoing varying acetylation for 30, 60, and 90 minutes (CAP1, CAP2, CAP3 conduits).
34142506	4	70	dep	minutes	767:773	arg1	conduits					793:800	CAP3 conduits	788:800	CAP3 conduits	788:800	Then the CP conduits with different acetyl degree were resulted undergoing varying acetylation for 30, 60, and 90 minutes (CAP1, CAP2, CAP3 conduits).
34142506	4	70	dep	minutes	767:773	arg1	CAP1					776:779	CAP1	776:779	CAP1	776:779	Then the CP conduits with different acetyl degree were resulted undergoing varying acetylation for 30, 60, and 90 minutes (CAP1, CAP2, CAP3 conduits).
34142506	15	71	theme	acetylation	2242:2252	arg1	degree					2232:2237	the degree	2228:2237	the degree of acetylation	2228:2252	SEM observation showed that there were more pores at 12 weeks after implantation, and the pores showed an increasing trend as the degree of acetylation increased.
34142506	7	72	theme	fascial	1036:1042	arg1	tunnels					1044:1050	the subcutaneous fascial tunnels	1019:1050	the subcutaneous fascial tunnels	1019:1050	The above conduits were implanted after the subcutaneous fascial tunnels were made symmetrically on both sides of the back of 30 female Sprague Dawley rats.
34142506	18	73	theme	acetylation	2655:2665	arg1	degrees					2667:2673	different acetylation degrees	2645:2673	different acetylation degrees	2645:2673	CONCLUSION The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees has good biocompatibility, conductivity, and biodegradability correlated with acetylation degree in vivo, which provide a new scaffold material for the construction of tissue engineered nerve.
34142506	9	74	theme	immunofluorescence	1352:1369	arg1	staining					1371:1378	anti-macrophage immunofluorescence staining	1336:1378	anti-macrophage immunofluorescence staining	1336:1378	HE staining and anti-macrophage immunofluorescence staining were performed to observe the histocompatibility in vivo.
34142506	3	75	theme	nano	590:593	arg1	conduit					618:624	the nano PPy/chitosan composite conduit	586:624	the nano PPy/chitosan composite conduit (CP conduit)	586:637	After freeze-drying and deacidification, the nano PPy/chitosan composite conduit (CP conduit) was prepared.
34142506	3	75	theme	nano	590:593	arg1	conduit					630:636	CP conduit	627:636	CP conduit	627:636	After freeze-drying and deacidification, the nano PPy/chitosan composite conduit (CP conduit) was prepared.
34142506	9	76	theme	anti-macrophage	1336:1350	arg1	staining					1371:1378	anti-macrophage immunofluorescence staining	1336:1378	anti-macrophage immunofluorescence staining	1336:1378	HE staining and anti-macrophage immunofluorescence staining were performed to observe the histocompatibility in vivo.
34142506	1	77	theme	conductive	213:222	arg1	conduit					240:246	conductive composite nerve conduit	213:246	conductive composite nerve conduit	213:246	OBJECTIVE To investigate the in vivo degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit, so as to provide a new scaffold material for the construction of tissue engineered nerve.
34142506	2	78	theme	conduit	520:526	arg1	model					538:542	a customized conduit formation model	507:542	a customized conduit formation model	507:542	METHODS The nano polypyrrole (PPy) was synthesized by microemulsion polymerization, blended with chitosan, and then formed conduit by injecting the mixed solution into a customized conduit formation model.
34142506	18	79	contain	has	2675:2677	arg1	conduit					2596:2602	The modified chitosan basedon conductive composite nerve conduit	2539:2602	The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees	2539:2673	CONCLUSION The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees has good biocompatibility, conductivity, and biodegradability correlated with acetylation degree in vivo, which provide a new scaffold material for the construction of tissue engineered nerve.
34142506	18	79	contain	has	2675:2677	arg2	biodegradability					2720:2735	biodegradability	2720:2735	biodegradability	2720:2735	CONCLUSION The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees has good biocompatibility, conductivity, and biodegradability correlated with acetylation degree in vivo, which provide a new scaffold material for the construction of tissue engineered nerve.
34142506	18	79	contain	has	2675:2677	arg2	conductivity					2702:2713	conductivity	2702:2713	conductivity	2702:2713	CONCLUSION The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees has good biocompatibility, conductivity, and biodegradability correlated with acetylation degree in vivo, which provide a new scaffold material for the construction of tissue engineered nerve.
34142506	18	79	contain	has	2675:2677	arg2	biocompatibility					2684:2699	good biocompatibility	2679:2699	good biocompatibility	2679:2699	CONCLUSION The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees has good biocompatibility, conductivity, and biodegradability correlated with acetylation degree in vivo, which provide a new scaffold material for the construction of tissue engineered nerve.
34142506	18	80	theme	new	2797:2799	arg1	material					2810:2817	a new scaffold material	2795:2817	a new scaffold material for the construction of tissue engineered nerve	2795:2865	CONCLUSION The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees has good biocompatibility, conductivity, and biodegradability correlated with acetylation degree in vivo, which provide a new scaffold material for the construction of tissue engineered nerve.
34142506	7	81	theme	above	983:987	arg1	conduits					989:996	The above conduits	979:996	The above conduits	979:996	The above conduits were implanted after the subcutaneous fascial tunnels were made symmetrically on both sides of the back of 30 female Sprague Dawley rats.
34142506	12	82	from	conduits	1744:1751	arg1	groups					1760:1765	all groups	1756:1765	all groups	1756:1765	SEM observation showed that the surfaces of the conduits in all groups were similar with relatively smooth surface and compact structure.
34142506	18	83	theme	composite	2580:2588	arg1	conduit					2596:2602	The modified chitosan basedon conductive composite nerve conduit	2539:2602	The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees	2539:2673	CONCLUSION The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees has good biocompatibility, conductivity, and biodegradability correlated with acetylation degree in vivo, which provide a new scaffold material for the construction of tissue engineered nerve.
34142506	18	84	theme	conductive	2569:2578	arg1	conduit					2596:2602	The modified chitosan basedon conductive composite nerve conduit	2539:2602	The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees	2539:2673	CONCLUSION The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees has good biocompatibility, conductivity, and biodegradability correlated with acetylation degree in vivo, which provide a new scaffold material for the construction of tissue engineered nerve.
34142506	5	85	used	used	885:888	arg2	spectrum					832:839	Fourier infrared absorption spectrum	804:839	Fourier infrared absorption spectrum	804:839	Fourier infrared absorption spectrum and scanning electron microscopy (SEM) were used to identify the conduits.
34142506	5	85	used	used	885:888	arg2	SEM					875:877	SEM	875:877	SEM	875:877	Fourier infrared absorption spectrum and scanning electron microscopy (SEM) were used to identify the conduits.
34142506	5	85	used	used	885:888	arg2	microscopy					863:872	scanning electron microscopy	845:872	scanning electron microscopy (SEM)	845:878	Fourier infrared absorption spectrum and scanning electron microscopy (SEM) were used to identify the conduits.
34142506	18	86	theme	basedon	2561:2567	arg1	conduit					2596:2602	The modified chitosan basedon conductive composite nerve conduit	2539:2602	The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees	2539:2673	CONCLUSION The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees has good biocompatibility, conductivity, and biodegradability correlated with acetylation degree in vivo, which provide a new scaffold material for the construction of tissue engineered nerve.
34142506	3	87	theme	composite	608:616	arg1	conduit					618:624	the nano PPy/chitosan composite conduit	586:624	the nano PPy/chitosan composite conduit (CP conduit)	586:637	After freeze-drying and deacidification, the nano PPy/chitosan composite conduit (CP conduit) was prepared.
34142506	3	87	theme	composite	608:616	arg1	conduit					630:636	CP conduit	627:636	CP conduit	627:636	After freeze-drying and deacidification, the nano PPy/chitosan composite conduit (CP conduit) was prepared.
34142506	5	88	theme	scanning	845:852	arg1	microscopy					863:872	scanning electron microscopy	845:872	scanning electron microscopy (SEM)	845:878	Fourier infrared absorption spectrum and scanning electron microscopy (SEM) were used to identify the conduits.
34142506	5	88	theme	scanning	845:852	arg1	SEM					875:877	SEM	875:877	SEM	875:877	Fourier infrared absorption spectrum and scanning electron microscopy (SEM) were used to identify the conduits.
34142506	0	89	theme	composite	85:93	arg1	conduit					101:107	conductive composite nerve conduit	74:107	conductive composite nerve conduit	74:107	[In vivo degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit].
34142506	6	90	theme	four-probe	953:962	arg1	conductometer					964:976	four-probe conductometer	953:976	four-probe conductometer	953:976	And the conductivity was measured by four-probe conductometer.
34142506	4	91	theme	different	679:687	arg1	degree					696:701	different acetyl degree	679:701	different acetyl degree	679:701	Then the CP conduits with different acetyl degree were resulted undergoing varying acetylation for 30, 60, and 90 minutes (CAP1, CAP2, CAP3 conduits).
34142506	18	92	theme	acetylation	2753:2763	arg1	degree					2765:2770	acetylation degree	2753:2770	acetylation degree	2753:2770	CONCLUSION The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees has good biocompatibility, conductivity, and biodegradability correlated with acetylation degree in vivo, which provide a new scaffold material for the construction of tissue engineered nerve.
34142506	14	93	theme	different	1989:1997	arg1	degrees					1999:2005	different degrees	1989:2005	different degrees of mass loss	1989:2018	All conduits had different degrees of mass loss, and the higher the degree of acetylation, the greater the mass change ( P<0.05).
34142506	12	94	theme	smooth	1796:1801	arg1	surface					1803:1809	relatively smooth surface	1785:1809	relatively smooth surface	1785:1809	SEM observation showed that the surfaces of the conduits in all groups were similar with relatively smooth surface and compact structure.
34142506	17	95	theme	implantation	2415:2426	arg1	time					2428:2431	implantation time	2415:2431	implantation time	2415:2431	With the extension of implantation time, lymphocytes decreased, fibroblasts increased, and collagen fibers proliferated significantly.
34142506	4	96	theme	varying	728:734	arg1	acetylation					736:746	varying acetylation	728:746	varying acetylation	728:746	Then the CP conduits with different acetyl degree were resulted undergoing varying acetylation for 30, 60, and 90 minutes (CAP1, CAP2, CAP3 conduits).
34142506	18	97	theme	modified	2543:2550	arg1	conduit					2596:2602	The modified chitosan basedon conductive composite nerve conduit	2539:2602	The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees	2539:2673	CONCLUSION The modified chitosan basedon conductive composite nerve conduit made of nano-PPy/chitosan composite with different acetylation degrees has good biocompatibility, conductivity, and biodegradability correlated with acetylation degree in vivo, which provide a new scaffold material for the construction of tissue engineered nerve.
34142506	1	98	theme	scaffold	272:279	arg1	material					281:288	a new scaffold material	266:288	a new scaffold material for the construction of tissue engineered nerve	266:336	OBJECTIVE To investigate the in vivo degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit, so as to provide a new scaffold material for the construction of tissue engineered nerve.
34142506	16	99	theme	lymphocytes	2334:2344	arg1	infiltration					2346:2357	more macrophages and lymphocytes infiltration	2313:2357	more macrophages and lymphocytes infiltration	2313:2357	Histological observation showed that there were more macrophages and lymphocytes infiltration in each group at the early stage.
34142506	14	100	dep	higher	2029:2034	arg1	degree					2040:2045	the degree	2036:2045	the higher the degree of acetylation, the greater the mass change ( P<0.05)	2025:2099	All conduits had different degrees of mass loss, and the higher the degree of acetylation, the greater the mass change ( P<0.05).
34142506	13	101	theme	time	1905:1908	arg1	extension					1892:1900	the extension	1888:1900	the extension of time	1888:1908	After the conduits were implanted into the rats, with the extension of time, all conduits were collapsed, especially on the CAP3 conduit.
34142506	0	102	theme	chitosan	56:63	arg1	degradation					9:19	[In vivo degradation	0:19	[In vivo degradation	0:19	[In vivo degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit].
34142506	0	102	theme	chitosan	56:63	arg1	histocompatibility					25:42	histocompatibility	25:42	histocompatibility	25:42	[In vivo degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit].
34142506	15	103	theme	SEM	2102:2104	arg1	observation					2106:2116	SEM observation	2102:2116	SEM observation	2102:2116	SEM observation showed that there were more pores at 12 weeks after implantation, and the pores showed an increasing trend as the degree of acetylation increased.
34142506	12	104	theme	conduits	1744:1751	arg1	similar					1772:1778	similar	1772:1778	similar	1772:1778	SEM observation showed that the surfaces of the conduits in all groups were similar with relatively smooth surface and compact structure.
34142506	12	104	theme	conduits	1744:1751	arg1	surfaces					1728:1735	the surfaces	1724:1735	the surfaces of the conduits in all groups	1724:1765	SEM observation showed that the surfaces of the conduits in all groups were similar with relatively smooth surface and compact structure.
34142506	8	105	theme	in	1287:1288	arg1	degradation					1295:1305	the in vivo degradation	1283:1305	the in vivo degradation of conduits	1283:1317	At 2, 4, 6, 8, 10, and 12 weeks after operation, the morphology, the microstructure, and the degradation rate were observed and measured to assess the in vivo degradation of conduits.
34142506	1	106	theme	tissue	314:319	arg1	construction					298:309	the construction	294:309	the construction of tissue engineered nerve	294:336	OBJECTIVE To investigate the in vivo degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit, so as to provide a new scaffold material for the construction of tissue engineered nerve.
34142506	10	107	theme	chitosan	1591:1598	arg1	successful					1604:1613	successful	1604:1613	successful	1604:1613	RESULTS The characteristic peaks of the amide Ⅱ band around 1 562 cm -1 appeared after being acetylated, indicating that the acetylation modification of chitosan was successful.
34142506	10	107	theme	chitosan	1591:1598	arg1	modification					1575:1586	the acetylation modification	1559:1586	the acetylation modification of chitosan	1559:1598	RESULTS The characteristic peaks of the amide Ⅱ band around 1 562 cm -1 appeared after being acetylated, indicating that the acetylation modification of chitosan was successful.
34142506	12	108	with	similar	1772:1778	arg1	surface					1803:1809	relatively smooth surface	1785:1809	relatively smooth surface	1785:1809	SEM observation showed that the surfaces of the conduits in all groups were similar with relatively smooth surface and compact structure.
34142506	12	108	with	similar	1772:1778	arg1	structure					1823:1831	compact structure	1815:1831	compact structure	1815:1831	SEM observation showed that the surfaces of the conduits in all groups were similar with relatively smooth surface and compact structure.
34142506	5	109	theme	Fourier	804:810	arg1	spectrum					832:839	Fourier infrared absorption spectrum	804:839	Fourier infrared absorption spectrum	804:839	Fourier infrared absorption spectrum and scanning electron microscopy (SEM) were used to identify the conduits.
34142506	8	110	theme	conduits	1310:1317	arg1	degradation					1295:1305	the in vivo degradation	1283:1305	the in vivo degradation of conduits	1283:1317	At 2, 4, 6, 8, 10, and 12 weeks after operation, the morphology, the microstructure, and the degradation rate were observed and measured to assess the in vivo degradation of conduits.
34142506	16	111	theme	Histological	2265:2276	arg1	observation					2278:2288	Histological observation	2265:2288	Histological observation	2265:2288	Histological observation showed that there were more macrophages and lymphocytes infiltration in each group at the early stage.
32891643	8	0	theme	in-vivo	1233:1239	arg1	test					1227:1230	wound healing test	1213:1230	wound healing test (in-vivo studies)	1213:1248	In wound healing test (in-vivo studies), this MC loaded composite film showed faster healing of the burn wound in rat compare to the control sample.
32891643	8	0	theme	in-vivo	1233:1239	arg1	studies					1241:1247	in-vivo studies	1233:1247	in-vivo studies	1233:1247	In wound healing test (in-vivo studies), this MC loaded composite film showed faster healing of the burn wound in rat compare to the control sample.
32891643	7	1	theme	C40P60MNT	1105:1113	arg1	sample					1115:1120	the C40P60MNT sample	1101:1120	the C40P60MNT sample	1101:1120	In-vitro antibacterial testing showed that the C40P60MNT sample had an acceptable effect on the inhibition of gram-positive and gram-negative bacteria.
32891643	2	2	mod	modified	273:280	arg1	composites					257:266	Halloysite nanotube (HNT) composites	231:266	Halloysite nanotube (HNT) composites	231:266	Halloysite nanotube (HNT) composites were modified with different concentration of both poly (lactic-co-glycolic acid) (PLGA) and chitosan (CS) through the Layer-by-Layer (LbL) strategy for targeted and controlled drug delivery of minocycline (MC).
32891643	2	2	mod	modified	273:280	arg3	concentration					297:309	different concentration	287:309	different concentration of both poly (lactic-co-glycolic acid) (PLGA) and chitosan (CS)	287:373	Halloysite nanotube (HNT) composites were modified with different concentration of both poly (lactic-co-glycolic acid) (PLGA) and chitosan (CS) through the Layer-by-Layer (LbL) strategy for targeted and controlled drug delivery of minocycline (MC).
32891643	4	3	theme	vapor	655:659	arg1	transmission					661:672	water vapor transmission	649:672	water vapor transmission	649:672	Studying the biodegradability, water uptake, photostability, and water vapor transmission of resulting composites revealed that the composite film absorbed wound secretions, did not degrade, and penetrated properly to wound during the treatment.
32891643	0	4	theme	controlled	96:105	arg1	release					107:113	controlled release	96:113	controlled release of minocycline	96:128	Layered biocompatible pH-responsive antibacterial composite film based on HNT/PLGA/chitosan for controlled release of minocycline as burn wound dressing.
32891643	7	5	theme	antibacterial	1067:1079	arg1	testing					1081:1087	In-vitro antibacterial testing	1058:1087	In-vitro antibacterial testing	1058:1087	In-vitro antibacterial testing showed that the C40P60MNT sample had an acceptable effect on the inhibition of gram-positive and gram-negative bacteria.
32891643	4	6	theme	water	649:653	arg1	transmission					661:672	water vapor transmission	649:672	water vapor transmission	649:672	Studying the biodegradability, water uptake, photostability, and water vapor transmission of resulting composites revealed that the composite film absorbed wound secretions, did not degrade, and penetrated properly to wound during the treatment.
32891643	0	7	theme	minocycline	118:128	arg1	release					107:113	controlled release	96:113	controlled release of minocycline	96:128	Layered biocompatible pH-responsive antibacterial composite film based on HNT/PLGA/chitosan for controlled release of minocycline as burn wound dressing.
32891643	9	8	theme	drug	1475:1478	arg1	delivery					1480:1487	pH-responsive drug delivery	1461:1487	pH-responsive drug delivery in the treatment of chronic burn wounds	1461:1527	Due to the characteristics of the optimized sample, it can be considered as a promising candidate for pH-responsive drug delivery in the treatment of chronic burn wounds.
32891643	7	9	contain	had	1122:1124	arg2	effect					1140:1145	an acceptable effect	1126:1145	an acceptable effect	1126:1145	In-vitro antibacterial testing showed that the C40P60MNT sample had an acceptable effect on the inhibition of gram-positive and gram-negative bacteria.
32891643	7	9	contain	had	1122:1124	arg1	sample					1115:1120	the C40P60MNT sample	1101:1120	the C40P60MNT sample	1101:1120	In-vitro antibacterial testing showed that the C40P60MNT sample had an acceptable effect on the inhibition of gram-positive and gram-negative bacteria.
32891643	5	10	theme	protein	845:851	arg1	adsorption					853:862	protein adsorption	845:862	protein adsorption	845:862	The results of protein adsorption showed that the optimized composite (C40P60MNT) was blood-compatible.
32891643	7	11	theme	bacteria	1200:1207	arg1	inhibition					1154:1163	the inhibition	1150:1163	the inhibition of gram-positive and gram-negative bacteria	1150:1207	In-vitro antibacterial testing showed that the C40P60MNT sample had an acceptable effect on the inhibition of gram-positive and gram-negative bacteria.
32891643	8	12	theme	burn	1310:1313	arg1	wound					1315:1319	the burn wound	1306:1319	the burn wound	1306:1319	In wound healing test (in-vivo studies), this MC loaded composite film showed faster healing of the burn wound in rat compare to the control sample.
32891643	5	13	theme	adsorption	853:862	arg1	results					834:840	The results	830:840	The results of protein adsorption	830:862	The results of protein adsorption showed that the optimized composite (C40P60MNT) was blood-compatible.
32891643	3	14	dep	FT-IR	527:531	arg1	studies					575:581	studies	575:581	studies	575:581	The resulting composites were characterized by FT-IR, XRD, zeta-potential, TGA, FE-SEM and TEM studies.
32891643	0	15	theme	burn	133:136	arg1	dressing					144:151	burn wound dressing	133:151	burn wound dressing	133:151	Layered biocompatible pH-responsive antibacterial composite film based on HNT/PLGA/chitosan for controlled release of minocycline as burn wound dressing.
32891643	8	16	theme	composite	1266:1274	arg1	film					1276:1279	this MC loaded composite film	1251:1279	this MC loaded composite film	1251:1279	In wound healing test (in-vivo studies), this MC loaded composite film showed faster healing of the burn wound in rat compare to the control sample.
32891643	2	17	theme	different	287:295	arg1	concentration					297:309	different concentration	287:309	different concentration of both poly (lactic-co-glycolic acid) (PLGA) and chitosan (CS)	287:373	Halloysite nanotube (HNT) composites were modified with different concentration of both poly (lactic-co-glycolic acid) (PLGA) and chitosan (CS) through the Layer-by-Layer (LbL) strategy for targeted and controlled drug delivery of minocycline (MC).
32891643	6	18	theme	kinetic	1043:1049	arg1	model					1051:1055	Korsmeyer-Peppas kinetic model	1026:1055	Korsmeyer-Peppas kinetic model	1026:1055	Studying the release profile of the drug showed pH-responsive behavior that was fitted with Korsmeyer-Peppas kinetic model.
32891643	9	19	theme	optimized	1393:1401	arg1	sample					1403:1408	the optimized sample	1389:1408	the optimized sample	1389:1408	Due to the characteristics of the optimized sample, it can be considered as a promising candidate for pH-responsive drug delivery in the treatment of chronic burn wounds.
32891643	6	20	theme	release	947:953	arg1	profile					955:961	the release profile	943:961	the release profile of the drug	943:973	Studying the release profile of the drug showed pH-responsive behavior that was fitted with Korsmeyer-Peppas kinetic model.
32891643	2	21	theme	targeted	421:428	arg1	delivery					450:457	targeted and controlled drug delivery	421:457	targeted and controlled drug delivery of minocycline (MC)	421:477	Halloysite nanotube (HNT) composites were modified with different concentration of both poly (lactic-co-glycolic acid) (PLGA) and chitosan (CS) through the Layer-by-Layer (LbL) strategy for targeted and controlled drug delivery of minocycline (MC).
32891643	1	22	theme	promising	178:186	arg1	dressing					208:215	a promising pH-responsive wound dressing	176:215	a promising pH-responsive wound dressing	176:215	In the present study, a promising pH-responsive wound dressing was prepared.
32891643	0	23	theme	biocompatible	8:20	arg1	film					60:63	Layered biocompatible pH-responsive antibacterial composite film	0:63	Layered biocompatible pH-responsive antibacterial composite film	0:63	Layered biocompatible pH-responsive antibacterial composite film based on HNT/PLGA/chitosan for controlled release of minocycline as burn wound dressing.
32891643	2	24	theme	minocycline	462:472	arg1	delivery					450:457	targeted and controlled drug delivery	421:457	targeted and controlled drug delivery of minocycline (MC)	421:477	Halloysite nanotube (HNT) composites were modified with different concentration of both poly (lactic-co-glycolic acid) (PLGA) and chitosan (CS) through the Layer-by-Layer (LbL) strategy for targeted and controlled drug delivery of minocycline (MC).
32891643	7	25	theme	In-vitro	1058:1065	arg1	testing					1081:1087	In-vitro antibacterial testing	1058:1087	In-vitro antibacterial testing	1058:1087	In-vitro antibacterial testing showed that the C40P60MNT sample had an acceptable effect on the inhibition of gram-positive and gram-negative bacteria.
32891643	9	26	theme	chronic	1509:1515	arg1	wounds					1522:1527	chronic burn wounds	1509:1527	chronic burn wounds	1509:1527	Due to the characteristics of the optimized sample, it can be considered as a promising candidate for pH-responsive drug delivery in the treatment of chronic burn wounds.
32891643	2	27	theme	lactic-co-glycolic	325:342	arg1	poly					319:322	poly	319:322	poly (lactic-co-glycolic acid) (PLGA)	319:355	Halloysite nanotube (HNT) composites were modified with different concentration of both poly (lactic-co-glycolic acid) (PLGA) and chitosan (CS) through the Layer-by-Layer (LbL) strategy for targeted and controlled drug delivery of minocycline (MC).
32891643	2	27	theme	lactic-co-glycolic	325:342	arg1	acid					344:347	lactic-co-glycolic acid	325:347	lactic-co-glycolic acid	325:347	Halloysite nanotube (HNT) composites were modified with different concentration of both poly (lactic-co-glycolic acid) (PLGA) and chitosan (CS) through the Layer-by-Layer (LbL) strategy for targeted and controlled drug delivery of minocycline (MC).
32891643	1	28	theme	pH-responsive	188:200	arg1	dressing					208:215	a promising pH-responsive wound dressing	176:215	a promising pH-responsive wound dressing	176:215	In the present study, a promising pH-responsive wound dressing was prepared.
32891643	0	29	theme	Layered	0:6	arg1	film					60:63	Layered biocompatible pH-responsive antibacterial composite film	0:63	Layered biocompatible pH-responsive antibacterial composite film	0:63	Layered biocompatible pH-responsive antibacterial composite film based on HNT/PLGA/chitosan for controlled release of minocycline as burn wound dressing.
32891643	9	30	theme	burn	1517:1520	arg1	wounds					1522:1527	chronic burn wounds	1509:1527	chronic burn wounds	1509:1527	Due to the characteristics of the optimized sample, it can be considered as a promising candidate for pH-responsive drug delivery in the treatment of chronic burn wounds.
32891643	4	31	theme	resulting	677:685	arg1	composites					687:696	resulting composites	677:696	resulting composites	677:696	Studying the biodegradability, water uptake, photostability, and water vapor transmission of resulting composites revealed that the composite film absorbed wound secretions, did not degrade, and penetrated properly to wound during the treatment.
32891643	8	32	from	healing	1295:1301	arg1	rat					1324:1326	rat	1324:1326	rat compare to the control sample	1324:1356	In wound healing test (in-vivo studies), this MC loaded composite film showed faster healing of the burn wound in rat compare to the control sample.
32891643	9	33	theme	wounds	1522:1527	arg1	treatment					1496:1504	the treatment	1492:1504	the treatment of chronic burn wounds	1492:1527	Due to the characteristics of the optimized sample, it can be considered as a promising candidate for pH-responsive drug delivery in the treatment of chronic burn wounds.
32891643	1	34	theme	wound	202:206	arg1	dressing					208:215	a promising pH-responsive wound dressing	176:215	a promising pH-responsive wound dressing	176:215	In the present study, a promising pH-responsive wound dressing was prepared.
32891643	0	35	theme	antibacterial	36:48	arg1	film					60:63	Layered biocompatible pH-responsive antibacterial composite film	0:63	Layered biocompatible pH-responsive antibacterial composite film	0:63	Layered biocompatible pH-responsive antibacterial composite film based on HNT/PLGA/chitosan for controlled release of minocycline as burn wound dressing.
32891643	5	36	theme	composite	890:898	arg1	C40P60MNT					901:909	the optimized composite (C40P60MNT)	876:910	the optimized composite (C40P60MNT)	876:910	The results of protein adsorption showed that the optimized composite (C40P60MNT) was blood-compatible.
32891643	5	36	theme	composite	890:898	arg1	blood-compatible					916:931	blood-compatible	916:931	blood-compatible	916:931	The results of protein adsorption showed that the optimized composite (C40P60MNT) was blood-compatible.
32891643	2	37	theme	poly	319:322	arg1	concentration					297:309	different concentration	287:309	different concentration of both poly (lactic-co-glycolic acid) (PLGA) and chitosan (CS)	287:373	Halloysite nanotube (HNT) composites were modified with different concentration of both poly (lactic-co-glycolic acid) (PLGA) and chitosan (CS) through the Layer-by-Layer (LbL) strategy for targeted and controlled drug delivery of minocycline (MC).
32891643	9	38	from	delivery	1480:1487	arg1	treatment					1496:1504	the treatment	1492:1504	the treatment of chronic burn wounds	1492:1527	Due to the characteristics of the optimized sample, it can be considered as a promising candidate for pH-responsive drug delivery in the treatment of chronic burn wounds.
32891643	8	39	dep	rat	1324:1326	arg1	compare					1328:1334	compare	1328:1334	compare to the control sample	1328:1356	In wound healing test (in-vivo studies), this MC loaded composite film showed faster healing of the burn wound in rat compare to the control sample.
32891643	0	40	theme	pH-responsive	22:34	arg1	film					60:63	Layered biocompatible pH-responsive antibacterial composite film	0:63	Layered biocompatible pH-responsive antibacterial composite film	0:63	Layered biocompatible pH-responsive antibacterial composite film based on HNT/PLGA/chitosan for controlled release of minocycline as burn wound dressing.
32891643	0	41	theme	wound	138:142	arg1	dressing					144:151	burn wound dressing	133:151	burn wound dressing	133:151	Layered biocompatible pH-responsive antibacterial composite film based on HNT/PLGA/chitosan for controlled release of minocycline as burn wound dressing.
32891643	2	42	theme	LbL	403:405	arg1	strategy					408:415	the Layer-by-Layer (LbL) strategy	383:415	the Layer-by-Layer (LbL) strategy for targeted and controlled drug delivery of minocycline (MC)	383:477	Halloysite nanotube (HNT) composites were modified with different concentration of both poly (lactic-co-glycolic acid) (PLGA) and chitosan (CS) through the Layer-by-Layer (LbL) strategy for targeted and controlled drug delivery of minocycline (MC).
32891643	7	43	theme	acceptable	1129:1138	arg1	effect					1140:1145	an acceptable effect	1126:1145	an acceptable effect	1126:1145	In-vitro antibacterial testing showed that the C40P60MNT sample had an acceptable effect on the inhibition of gram-positive and gram-negative bacteria.
32891643	9	44	theme	promising	1437:1445	arg1	candidate					1447:1455	a promising candidate	1435:1455	a promising candidate for pH-responsive drug delivery in the treatment of chronic burn wounds	1435:1527	Due to the characteristics of the optimized sample, it can be considered as a promising candidate for pH-responsive drug delivery in the treatment of chronic burn wounds.
32891643	9	44	theme	promising	1437:1445	arg1	it					1411:1412	it	1411:1412	it	1411:1412	Due to the characteristics of the optimized sample, it can be considered as a promising candidate for pH-responsive drug delivery in the treatment of chronic burn wounds.
32891643	2	45	theme	HNT	252:254	arg1	composites					257:266	Halloysite nanotube (HNT) composites	231:266	Halloysite nanotube (HNT) composites	231:266	Halloysite nanotube (HNT) composites were modified with different concentration of both poly (lactic-co-glycolic acid) (PLGA) and chitosan (CS) through the Layer-by-Layer (LbL) strategy for targeted and controlled drug delivery of minocycline (MC).
32891643	6	46	theme	Korsmeyer-Peppas	1026:1041	arg1	model					1051:1055	Korsmeyer-Peppas kinetic model	1026:1055	Korsmeyer-Peppas kinetic model	1026:1055	Studying the release profile of the drug showed pH-responsive behavior that was fitted with Korsmeyer-Peppas kinetic model.
32891643	0	47	theme	composite	50:58	arg1	film					60:63	Layered biocompatible pH-responsive antibacterial composite film	0:63	Layered biocompatible pH-responsive antibacterial composite film	0:63	Layered biocompatible pH-responsive antibacterial composite film based on HNT/PLGA/chitosan for controlled release of minocycline as burn wound dressing.
32891643	4	48	dep	biodegradability	597:612	arg1	the					593:595	the	593:595	the	593:595	Studying the biodegradability, water uptake, photostability, and water vapor transmission of resulting composites revealed that the composite film absorbed wound secretions, did not degrade, and penetrated properly to wound during the treatment.
32891643	2	49	theme	Layer-by-Layer	387:400	arg1	strategy					408:415	the Layer-by-Layer (LbL) strategy	383:415	the Layer-by-Layer (LbL) strategy for targeted and controlled drug delivery of minocycline (MC)	383:477	Halloysite nanotube (HNT) composites were modified with different concentration of both poly (lactic-co-glycolic acid) (PLGA) and chitosan (CS) through the Layer-by-Layer (LbL) strategy for targeted and controlled drug delivery of minocycline (MC).
32891643	2	50	theme	drug	445:448	arg1	delivery					450:457	targeted and controlled drug delivery	421:457	targeted and controlled drug delivery of minocycline (MC)	421:477	Halloysite nanotube (HNT) composites were modified with different concentration of both poly (lactic-co-glycolic acid) (PLGA) and chitosan (CS) through the Layer-by-Layer (LbL) strategy for targeted and controlled drug delivery of minocycline (MC).
32891643	7	51	theme	gram-negative	1186:1198	arg1	bacteria					1200:1207	gram-positive and gram-negative bacteria	1168:1207	gram-positive and gram-negative bacteria	1168:1207	In-vitro antibacterial testing showed that the C40P60MNT sample had an acceptable effect on the inhibition of gram-positive and gram-negative bacteria.
32891643	2	52	theme	nanotube	242:249	arg1	composites					257:266	Halloysite nanotube (HNT) composites	231:266	Halloysite nanotube (HNT) composites	231:266	Halloysite nanotube (HNT) composites were modified with different concentration of both poly (lactic-co-glycolic acid) (PLGA) and chitosan (CS) through the Layer-by-Layer (LbL) strategy for targeted and controlled drug delivery of minocycline (MC).
32891643	8	53	theme	faster	1288:1293	arg1	healing					1295:1301	faster healing	1288:1301	faster healing of the burn wound in rat compare to the control sample	1288:1356	In wound healing test (in-vivo studies), this MC loaded composite film showed faster healing of the burn wound in rat compare to the control sample.
32891643	2	54	theme	controlled	434:443	arg1	delivery					450:457	targeted and controlled drug delivery	421:457	targeted and controlled drug delivery of minocycline (MC)	421:477	Halloysite nanotube (HNT) composites were modified with different concentration of both poly (lactic-co-glycolic acid) (PLGA) and chitosan (CS) through the Layer-by-Layer (LbL) strategy for targeted and controlled drug delivery of minocycline (MC).
32891643	2	55	theme	Halloysite	231:240	arg1	composites					257:266	Halloysite nanotube (HNT) composites	231:266	Halloysite nanotube (HNT) composites	231:266	Halloysite nanotube (HNT) composites were modified with different concentration of both poly (lactic-co-glycolic acid) (PLGA) and chitosan (CS) through the Layer-by-Layer (LbL) strategy for targeted and controlled drug delivery of minocycline (MC).
32891643	8	56	theme	loaded	1259:1264	arg1	film					1276:1279	this MC loaded composite film	1251:1279	this MC loaded composite film	1251:1279	In wound healing test (in-vivo studies), this MC loaded composite film showed faster healing of the burn wound in rat compare to the control sample.
32891643	6	57	theme	pH-responsive	982:994	arg1	behavior					996:1003	pH-responsive behavior	982:1003	pH-responsive behavior that was fitted with Korsmeyer-Peppas kinetic model	982:1055	Studying the release profile of the drug showed pH-responsive behavior that was fitted with Korsmeyer-Peppas kinetic model.
32891643	4	58	theme	composites	687:696	arg1	biodegradability					597:612	biodegradability	597:612	biodegradability	597:612	Studying the biodegradability, water uptake, photostability, and water vapor transmission of resulting composites revealed that the composite film absorbed wound secretions, did not degrade, and penetrated properly to wound during the treatment.
32891643	4	58	theme	composites	687:696	arg1	uptake					621:626	water uptake	615:626	water uptake	615:626	Studying the biodegradability, water uptake, photostability, and water vapor transmission of resulting composites revealed that the composite film absorbed wound secretions, did not degrade, and penetrated properly to wound during the treatment.
32891643	4	58	theme	composites	687:696	arg1	transmission					661:672	water vapor transmission	649:672	water vapor transmission	649:672	Studying the biodegradability, water uptake, photostability, and water vapor transmission of resulting composites revealed that the composite film absorbed wound secretions, did not degrade, and penetrated properly to wound during the treatment.
32891643	4	58	theme	composites	687:696	arg1	photostability					629:642	photostability	629:642	photostability	629:642	Studying the biodegradability, water uptake, photostability, and water vapor transmission of resulting composites revealed that the composite film absorbed wound secretions, did not degrade, and penetrated properly to wound during the treatment.
32891643	4	59	theme	composite	716:724	arg1	film					726:729	the composite film	712:729	the composite film	712:729	Studying the biodegradability, water uptake, photostability, and water vapor transmission of resulting composites revealed that the composite film absorbed wound secretions, did not degrade, and penetrated properly to wound during the treatment.
32891643	9	60	theme	sample	1403:1408	arg1	characteristics					1370:1384	the characteristics	1366:1384	the characteristics of the optimized sample	1366:1408	Due to the characteristics of the optimized sample, it can be considered as a promising candidate for pH-responsive drug delivery in the treatment of chronic burn wounds.
32891643	4	61	theme	wound	740:744	arg1	secretions					746:755	wound secretions	740:755	wound secretions	740:755	Studying the biodegradability, water uptake, photostability, and water vapor transmission of resulting composites revealed that the composite film absorbed wound secretions, did not degrade, and penetrated properly to wound during the treatment.
32891643	8	62	theme	wound	1213:1217	arg1	healing					1219:1225	wound healing	1213:1225	wound healing test (in-vivo studies)	1213:1248	In wound healing test (in-vivo studies), this MC loaded composite film showed faster healing of the burn wound in rat compare to the control sample.
32891643	8	63	theme	wound	1315:1319	arg1	healing					1295:1301	faster healing	1288:1301	faster healing of the burn wound in rat compare to the control sample	1288:1356	In wound healing test (in-vivo studies), this MC loaded composite film showed faster healing of the burn wound in rat compare to the control sample.
32891643	1	64	theme	present	161:167	arg1	study					169:173	the present study	157:173	the present study	157:173	In the present study, a promising pH-responsive wound dressing was prepared.
32891643	5	65	theme	optimized	880:888	arg1	C40P60MNT					901:909	the optimized composite (C40P60MNT)	876:910	the optimized composite (C40P60MNT)	876:910	The results of protein adsorption showed that the optimized composite (C40P60MNT) was blood-compatible.
32891643	5	65	theme	optimized	880:888	arg1	blood-compatible					916:931	blood-compatible	916:931	blood-compatible	916:931	The results of protein adsorption showed that the optimized composite (C40P60MNT) was blood-compatible.
32891643	8	66	theme	control	1343:1349	arg1	sample					1351:1356	the control sample	1339:1356	the control sample	1339:1356	In wound healing test (in-vivo studies), this MC loaded composite film showed faster healing of the burn wound in rat compare to the control sample.
32891643	9	67	theme	pH-responsive	1461:1473	arg1	delivery					1480:1487	pH-responsive drug delivery	1461:1487	pH-responsive drug delivery in the treatment of chronic burn wounds	1461:1527	Due to the characteristics of the optimized sample, it can be considered as a promising candidate for pH-responsive drug delivery in the treatment of chronic burn wounds.
32891643	3	68	theme	resulting	484:492	arg1	composites					494:503	The resulting composites	480:503	The resulting composites	480:503	The resulting composites were characterized by FT-IR, XRD, zeta-potential, TGA, FE-SEM and TEM studies.
32891643	4	69	theme	water	615:619	arg1	uptake					621:626	water uptake	615:626	water uptake	615:626	Studying the biodegradability, water uptake, photostability, and water vapor transmission of resulting composites revealed that the composite film absorbed wound secretions, did not degrade, and penetrated properly to wound during the treatment.
32891643	8	70	theme	healing	1219:1225	arg1	test					1227:1230	wound healing test	1213:1230	wound healing test (in-vivo studies)	1213:1248	In wound healing test (in-vivo studies), this MC loaded composite film showed faster healing of the burn wound in rat compare to the control sample.
32891643	8	70	theme	healing	1219:1225	arg1	studies					1241:1247	in-vivo studies	1233:1247	in-vivo studies	1233:1247	In wound healing test (in-vivo studies), this MC loaded composite film showed faster healing of the burn wound in rat compare to the control sample.
32891643	7	71	theme	gram-positive	1168:1180	arg1	bacteria					1200:1207	gram-positive and gram-negative bacteria	1168:1207	gram-positive and gram-negative bacteria	1168:1207	In-vitro antibacterial testing showed that the C40P60MNT sample had an acceptable effect on the inhibition of gram-positive and gram-negative bacteria.
32891643	8	72	dep	loaded	1259:1264	arg1	MC					1256:1257	MC	1256:1257	MC	1256:1257	In wound healing test (in-vivo studies), this MC loaded composite film showed faster healing of the burn wound in rat compare to the control sample.
32891643	2	73	theme	chitosan	361:368	arg1	concentration					297:309	different concentration	287:309	different concentration of both poly (lactic-co-glycolic acid) (PLGA) and chitosan (CS)	287:373	Halloysite nanotube (HNT) composites were modified with different concentration of both poly (lactic-co-glycolic acid) (PLGA) and chitosan (CS) through the Layer-by-Layer (LbL) strategy for targeted and controlled drug delivery of minocycline (MC).
32891643	6	74	theme	drug	970:973	arg1	profile					955:961	the release profile	943:961	the release profile of the drug	943:973	Studying the release profile of the drug showed pH-responsive behavior that was fitted with Korsmeyer-Peppas kinetic model.
32561577	6	0	theme	functional	1128:1137	arg1	analysis					1139:1146	functional analysis	1128:1146	functional analysis	1128:1146	However, functional analysis indicated that lactate, acetate, and formate evenly dominated the metabolic profile for all sugars except for xylitol.
32561577	11	1	contain	containing	2372:2381	arg1	communities					2360:2370	communities	2360:2370	communities containing lactobacilli and A. viscosus as well as with complex communities of bacteria from human saliva	2360:2476	While the resistance of kojibiose was established with monocultures, delayed utilization of kojibiose was observed with communities containing lactobacilli and A. viscosus as well as with complex communities of bacteria from human saliva.
32561577	11	1	contain	containing	2372:2381	arg2	communities					2436:2446	complex communities	2428:2446	complex communities of bacteria from human saliva	2428:2476	While the resistance of kojibiose was established with monocultures, delayed utilization of kojibiose was observed with communities containing lactobacilli and A. viscosus as well as with complex communities of bacteria from human saliva.
32561577	11	1	contain	containing	2372:2381	arg2	viscosus					2403:2410	A. viscosus	2400:2410	A. viscosus	2400:2410	While the resistance of kojibiose was established with monocultures, delayed utilization of kojibiose was observed with communities containing lactobacilli and A. viscosus as well as with complex communities of bacteria from human saliva.
32561577	11	1	contain	containing	2372:2381	arg2	lactobacilli					2383:2394	lactobacilli	2383:2394	lactobacilli	2383:2394	While the resistance of kojibiose was established with monocultures, delayed utilization of kojibiose was observed with communities containing lactobacilli and A. viscosus as well as with complex communities of bacteria from human saliva.
32561577	10	2	theme	xylitol	1872:1878	arg1	incubations					1880:1890	kojibiose and xylitol incubations	1858:1890	kojibiose and xylitol incubations	1858:1890	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	0	3	with	Incubation	91:100	arg1	Sucrose					107:113	Sucrose	107:113	Sucrose	107:113	Oral Microbiota Display Profound Differential Metabolic Kinetics and Community Shifts upon Incubation with Sucrose, Trehalose, Kojibiose, and Xylitol.
32561577	0	3	with	Incubation	91:100	arg1	Kojibiose					127:135	Kojibiose	127:135	Kojibiose	127:135	Oral Microbiota Display Profound Differential Metabolic Kinetics and Community Shifts upon Incubation with Sucrose, Trehalose, Kojibiose, and Xylitol.
32561577	0	3	with	Incubation	91:100	arg1	Xylitol					142:148	Xylitol	142:148	Xylitol	142:148	Oral Microbiota Display Profound Differential Metabolic Kinetics and Community Shifts upon Incubation with Sucrose, Trehalose, Kojibiose, and Xylitol.
32561577	0	3	with	Incubation	91:100	arg1	Trehalose					116:124	Trehalose	116:124	Trehalose	116:124	Oral Microbiota Display Profound Differential Metabolic Kinetics and Community Shifts upon Incubation with Sucrose, Trehalose, Kojibiose, and Xylitol.
32561577	2	4	with	bacteria	597:604	arg1	communities					564:574	Lactobacillus-based communities	544:574	Lactobacillus-based communities	544:574	Compared to sucrose and trehalose, kojibiose resisted metabolism during a 48-h incubation with monocultures, except for Actinomyces viscosus Incubations with Lactobacillus-based communities, as well as salivary bacteria, displayed kojibiose metabolism, yet to a lesser extent than sucrose and trehalose.
32561577	8	5	dep	sucrose	1540:1546	arg1	deviation					1565:1573	mean ± standard deviation	1549:1573	mean ± standard deviation	1549:1573	In Streptococcus spp., a taxon linked to cariogenesis dominated in sucrose (mean ± standard deviation, 91.8 ± 6.4%) and trehalose (55.9 ± 38.6%), representing a high diversity loss.
32561577	8	5	dep	sucrose	1540:1546	arg1	%					1586:1586	91.8 ± 6.4%	1576:1586	91.8 ± 6.4%	1576:1586	In Streptococcus spp., a taxon linked to cariogenesis dominated in sucrose (mean ± standard deviation, 91.8 ± 6.4%) and trehalose (55.9 ± 38.6%), representing a high diversity loss.
32561577	10	6	dep	properties.IMPORTANCE	2060:2080	arg1	provides					2093:2100	provides	2093:2100	provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible	2093:2237	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	14	7	theme	mixed	2889:2893	arg1	cultures					2895:2902	ex vivo mixed cultures	2881:2902	ex vivo mixed cultures	2881:2902	This study furthermore underscores the importance and relevance of microbial culture and ex vivo mixed cultures to study cariogenicity and substrate utilization; this is in sharp contrast with tests that solely rely on monocultures such as Streptococcus mutans, which clearly fail to capture complex interactions between oral microbiota.
32561577	7	8	theme	rRNA	1271:1274	arg1	analysis					1292:1299	16S rRNA gene sequencing analysis	1267:1299	16S rRNA gene sequencing analysis	1267:1299	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	5	9	from	differences	1031:1041	arg1	drop					1049:1052	pH drop	1046:1052	pH drop during incubation	1046:1070	Donor-dependent differences in salivary microbiome composition were noted, and differences in pH drop during incubation indicated different rates of sugar metabolism.
32561577	14	10	theme	ex	2881:2882	arg1	cultures					2895:2902	ex vivo mixed cultures	2881:2902	ex vivo mixed cultures	2881:2902	This study furthermore underscores the importance and relevance of microbial culture and ex vivo mixed cultures to study cariogenicity and substrate utilization; this is in sharp contrast with tests that solely rely on monocultures such as Streptococcus mutans, which clearly fail to capture complex interactions between oral microbiota.
32561577	10	11	theme	scientific	2113:2122	arg1	insight					2124:2130	a detailed scientific insight	2102:2130	a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible	2102:2237	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	4	12	dep	families	866:873	arg1	families					866:873	glycohydrolase (GH) families GH65 and GH15	846:887	glycohydrolase (GH) families GH65 and GH15	846:887	and A. viscosus possess enzymes from glycohydrolase (GH) families GH65 and GH15, respectively, which are associated with kojibiose metabolism.
32561577	4	12	dep	families	866:873	arg1	GH15					884:887	GH15	884:887	GH15	884:887	and A. viscosus possess enzymes from glycohydrolase (GH) families GH65 and GH15, respectively, which are associated with kojibiose metabolism.
32561577	4	12	dep	families	866:873	arg1	GH65					875:878	GH65	875:878	GH65	875:878	and A. viscosus possess enzymes from glycohydrolase (GH) families GH65 and GH15, respectively, which are associated with kojibiose metabolism.
32561577	3	13	theme	only	785:788	arg1	Lactobacillus					790:802	only Lactobacillus	785:802	only Lactobacillus	785:802	Concurring with our in vitro findings, screening for carbohydrate-active enzymes revealed that only Lactobacillus spp.
32561577	13	14	from	shifts	2605:2610	arg1	communities					2634:2644	salivary microbial communities	2615:2644	salivary microbial communities	2615:2644	Moreover, although conventional sugars cause distinct shifts in salivary microbial communities, our study has revealed that kojibiose is able to closely maintain the salivary microbiome composition, suggesting its low cariogenic properties.
32561577	14	15	theme	culture	2869:2875	arg1	relevance					2846:2854	relevance	2846:2854	relevance	2846:2854	This study furthermore underscores the importance and relevance of microbial culture and ex vivo mixed cultures to study cariogenicity and substrate utilization; this is in sharp contrast with tests that solely rely on monocultures such as Streptococcus mutans, which clearly fail to capture complex interactions between oral microbiota.
32561577	14	15	theme	culture	2869:2875	arg1	importance					2831:2840	importance	2831:2840	importance	2831:2840	This study furthermore underscores the importance and relevance of microbial culture and ex vivo mixed cultures to study cariogenicity and substrate utilization; this is in sharp contrast with tests that solely rely on monocultures such as Streptococcus mutans, which clearly fail to capture complex interactions between oral microbiota.
32561577	4	16	theme	kojibiose	930:938	arg1	metabolism					940:949	kojibiose metabolism	930:949	kojibiose metabolism	930:949	and A. viscosus possess enzymes from glycohydrolase (GH) families GH65 and GH15, respectively, which are associated with kojibiose metabolism.
32561577	3	17	theme	carbohydrate-active	743:761	arg1	enzymes					763:769	carbohydrate-active enzymes	743:769	carbohydrate-active enzymes	743:769	Concurring with our in vitro findings, screening for carbohydrate-active enzymes revealed that only Lactobacillus spp.
32561577	5	18	theme	sugar	1101:1105	arg1	metabolism					1107:1116	sugar metabolism	1101:1116	sugar metabolism	1101:1116	Donor-dependent differences in salivary microbiome composition were noted, and differences in pH drop during incubation indicated different rates of sugar metabolism.
32561577	14	19	theme	oral	3113:3116	arg1	microbiota					3118:3127	oral microbiota	3113:3127	oral microbiota	3113:3127	This study furthermore underscores the importance and relevance of microbial culture and ex vivo mixed cultures to study cariogenicity and substrate utilization; this is in sharp contrast with tests that solely rely on monocultures such as Streptococcus mutans, which clearly fail to capture complex interactions between oral microbiota.
32561577	1	20	theme	kojibiose	199:207	arg1	properties					185:194	the metabolic properties	171:194	the metabolic properties of kojibiose, trehalose, sucrose, and xylitol	171:240	This study compares the metabolic properties of kojibiose, trehalose, sucrose, and xylitol upon incubation with representative oral bacteria as monocultures or synthetic communities or with human salivary bacteria in a defined medium.
32561577	11	21	from	saliva	2471:2476	arg1	lactobacilli					2383:2394	lactobacilli	2383:2394	lactobacilli	2383:2394	While the resistance of kojibiose was established with monocultures, delayed utilization of kojibiose was observed with communities containing lactobacilli and A. viscosus as well as with complex communities of bacteria from human saliva.
32561577	11	21	from	saliva	2471:2476	arg1	bacteria					2451:2458	bacteria	2451:2458	bacteria from human saliva	2451:2476	While the resistance of kojibiose was established with monocultures, delayed utilization of kojibiose was observed with communities containing lactobacilli and A. viscosus as well as with complex communities of bacteria from human saliva.
32561577	11	21	from	saliva	2471:2476	arg1	communities					2436:2446	complex communities	2428:2446	complex communities of bacteria from human saliva	2428:2476	While the resistance of kojibiose was established with monocultures, delayed utilization of kojibiose was observed with communities containing lactobacilli and A. viscosus as well as with complex communities of bacteria from human saliva.
32561577	1	22	theme	trehalose	210:218	arg1	properties					185:194	the metabolic properties	171:194	the metabolic properties of kojibiose, trehalose, sucrose, and xylitol	171:240	This study compares the metabolic properties of kojibiose, trehalose, sucrose, and xylitol upon incubation with representative oral bacteria as monocultures or synthetic communities or with human salivary bacteria in a defined medium.
32561577	10	23	theme	disaccharide	2160:2171	arg1	metabolism					2139:2148	the metabolism	2135:2148	the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible	2135:2237	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	10	24	theme	key	1966:1968	arg1	phyla					1970:1974	key phyla	1966:1974	key phyla	1966:1974	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	7	25	from	trehalose	1432:1440	arg1	shift					1353:1357	a significant shift	1339:1357	a significant shift of the microbiome community by sugars	1339:1395	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	7	25	from	trehalose	1432:1440	arg1	pronounced					1406:1415	pronounced	1406:1415	pronounced	1406:1415	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	10	26	theme	salivary	1994:2001	arg1	microbiome					2003:2012	the salivary microbiome	1990:2012	the salivary microbiome	1990:2012	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	14	27	theme	cultures	2895:2902	arg1	relevance					2846:2854	relevance	2846:2854	relevance	2846:2854	This study furthermore underscores the importance and relevance of microbial culture and ex vivo mixed cultures to study cariogenicity and substrate utilization; this is in sharp contrast with tests that solely rely on monocultures such as Streptococcus mutans, which clearly fail to capture complex interactions between oral microbiota.
32561577	14	27	theme	cultures	2895:2902	arg1	importance					2831:2840	importance	2831:2840	importance	2831:2840	This study furthermore underscores the importance and relevance of microbial culture and ex vivo mixed cultures to study cariogenicity and substrate utilization; this is in sharp contrast with tests that solely rely on monocultures such as Streptococcus mutans, which clearly fail to capture complex interactions between oral microbiota.
32561577	11	28	theme	human	2465:2469	arg1	saliva					2471:2476	human saliva	2465:2476	human saliva	2465:2476	While the resistance of kojibiose was established with monocultures, delayed utilization of kojibiose was observed with communities containing lactobacilli and A. viscosus as well as with complex communities of bacteria from human saliva.
32561577	7	29	theme	16S	1267:1269	arg1	analysis					1292:1299	16S rRNA gene sequencing analysis	1267:1299	16S rRNA gene sequencing analysis	1267:1299	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	13	30	theme	salivary	2615:2622	arg1	communities					2634:2644	salivary microbial communities	2615:2644	salivary microbial communities	2615:2644	Moreover, although conventional sugars cause distinct shifts in salivary microbial communities, our study has revealed that kojibiose is able to closely maintain the salivary microbiome composition, suggesting its low cariogenic properties.
32561577	11	31	theme	bacteria	2451:2458	arg1	lactobacilli					2383:2394	lactobacilli	2383:2394	lactobacilli	2383:2394	While the resistance of kojibiose was established with monocultures, delayed utilization of kojibiose was observed with communities containing lactobacilli and A. viscosus as well as with complex communities of bacteria from human saliva.
32561577	11	31	theme	bacteria	2451:2458	arg1	communities					2436:2446	complex communities	2428:2446	complex communities of bacteria from human saliva	2428:2476	While the resistance of kojibiose was established with monocultures, delayed utilization of kojibiose was observed with communities containing lactobacilli and A. viscosus as well as with complex communities of bacteria from human saliva.
32561577	1	32	theme	human	341:345	arg1	bacteria					356:363	human salivary bacteria	341:363	human salivary bacteria	341:363	This study compares the metabolic properties of kojibiose, trehalose, sucrose, and xylitol upon incubation with representative oral bacteria as monocultures or synthetic communities or with human salivary bacteria in a defined medium.
32561577	14	33	theme	Streptococcus	3032:3044	arg1	mutans					3046:3051	Streptococcus mutans	3032:3051	Streptococcus mutans	3032:3051	This study furthermore underscores the importance and relevance of microbial culture and ex vivo mixed cultures to study cariogenicity and substrate utilization; this is in sharp contrast with tests that solely rely on monocultures such as Streptococcus mutans, which clearly fail to capture complex interactions between oral microbiota.
32561577	9	34	from	kojibiose	1716:1724	arg1	abundant					1704:1711	abundant	1704:1711	abundant	1704:1711	In contrast, Streptococcus (5.1 ± 3.7%) was less abundant in kojibiose, which instead was dominated by Veillonella (26.8 ± 19.6%), while for xylitol, Neisseria (29.4 ± 19.1%) was most abundant.
32561577	9	35	from	abundant	1704:1711	arg1	contrast					1658:1665	contrast	1658:1665	contrast	1658:1665	In contrast, Streptococcus (5.1 ± 3.7%) was less abundant in kojibiose, which instead was dominated by Veillonella (26.8 ± 19.6%), while for xylitol, Neisseria (29.4 ± 19.1%) was most abundant.
32561577	9	35	from	abundant	1704:1711	arg1	kojibiose					1716:1724	kojibiose	1716:1724	kojibiose	1716:1724	In contrast, Streptococcus (5.1 ± 3.7%) was less abundant in kojibiose, which instead was dominated by Veillonella (26.8 ± 19.6%), while for xylitol, Neisseria (29.4 ± 19.1%) was most abundant.
32561577	2	36	theme	viscosus	518:525	arg1	Incubations					527:537	Actinomyces viscosus Incubations	506:537	Actinomyces viscosus Incubations	506:537	Compared to sucrose and trehalose, kojibiose resisted metabolism during a 48-h incubation with monocultures, except for Actinomyces viscosus Incubations with Lactobacillus-based communities, as well as salivary bacteria, displayed kojibiose metabolism, yet to a lesser extent than sucrose and trehalose.
32561577	8	37	theme	mean ± standard	1549:1563	arg1	deviation					1565:1573	mean ± standard deviation	1549:1573	mean ± standard deviation	1549:1573	In Streptococcus spp., a taxon linked to cariogenesis dominated in sucrose (mean ± standard deviation, 91.8 ± 6.4%) and trehalose (55.9 ± 38.6%), representing a high diversity loss.
32561577	8	37	theme	mean ± standard	1549:1563	arg1	%					1586:1586	91.8 ± 6.4%	1576:1586	91.8 ± 6.4%	1576:1586	In Streptococcus spp., a taxon linked to cariogenesis dominated in sucrose (mean ± standard deviation, 91.8 ± 6.4%) and trehalose (55.9 ± 38.6%), representing a high diversity loss.
32561577	2	38	theme	lesser	648:653	arg1	extent					655:660	a lesser extent	646:660	a lesser extent	646:660	Compared to sucrose and trehalose, kojibiose resisted metabolism during a 48-h incubation with monocultures, except for Actinomyces viscosus Incubations with Lactobacillus-based communities, as well as salivary bacteria, displayed kojibiose metabolism, yet to a lesser extent than sucrose and trehalose.
32561577	7	39	theme	community	1377:1385	arg1	shift					1353:1357	a significant shift	1339:1357	a significant shift of the microbiome community by sugars	1339:1395	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	7	39	theme	community	1377:1385	arg1	pronounced					1406:1415	pronounced	1406:1415	pronounced	1406:1415	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	14	40	dep	ex	2881:2882	arg1	vivo					2884:2887	vivo	2884:2887	vivo	2884:2887	This study furthermore underscores the importance and relevance of microbial culture and ex vivo mixed cultures to study cariogenicity and substrate utilization; this is in sharp contrast with tests that solely rely on monocultures such as Streptococcus mutans, which clearly fail to capture complex interactions between oral microbiota.
32561577	0	41	theme	Oral	0:3	arg1	Kinetics					56:63	Oral Microbiota Display Profound Differential Metabolic Kinetics	0:63	Oral Microbiota Display Profound Differential Metabolic Kinetics	0:63	Oral Microbiota Display Profound Differential Metabolic Kinetics and Community Shifts upon Incubation with Sucrose, Trehalose, Kojibiose, and Xylitol.
32561577	1	42	theme	representative	263:276	arg1	bacteria					283:290	representative oral bacteria	263:290	representative oral bacteria as monocultures or synthetic communities	263:331	This study compares the metabolic properties of kojibiose, trehalose, sucrose, and xylitol upon incubation with representative oral bacteria as monocultures or synthetic communities or with human salivary bacteria in a defined medium.
32561577	7	43	from	pronounced	1406:1415	arg1	xylitol					1464:1470	xylitol	1464:1470	xylitol	1464:1470	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	7	43	from	pronounced	1406:1415	arg1	trehalose					1432:1440	trehalose	1432:1440	trehalose	1432:1440	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	7	43	from	pronounced	1406:1415	arg1	kojibiose					1450:1458	kojibiose	1450:1458	kojibiose	1450:1458	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	7	43	from	pronounced	1406:1415	arg1	sucrose					1420:1426	sucrose	1420:1426	sucrose	1420:1426	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	0	44	theme	Display	16:22	arg1	Kinetics					56:63	Oral Microbiota Display Profound Differential Metabolic Kinetics	0:63	Oral Microbiota Display Profound Differential Metabolic Kinetics	0:63	Oral Microbiota Display Profound Differential Metabolic Kinetics and Community Shifts upon Incubation with Sucrose, Trehalose, Kojibiose, and Xylitol.
32561577	3	45	dep	in	710:711	arg1	vitro					713:717	vitro	713:717	vitro	713:717	Concurring with our in vitro findings, screening for carbohydrate-active enzymes revealed that only Lactobacillus spp.
32561577	5	46	theme	salivary	983:990	arg1	composition					1003:1013	salivary microbiome composition	983:1013	salivary microbiome composition	983:1013	Donor-dependent differences in salivary microbiome composition were noted, and differences in pH drop during incubation indicated different rates of sugar metabolism.
32561577	7	47	from	sucrose	1420:1426	arg1	shift					1353:1357	a significant shift	1339:1357	a significant shift of the microbiome community by sugars	1339:1395	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	7	47	from	sucrose	1420:1426	arg1	pronounced					1406:1415	pronounced	1406:1415	pronounced	1406:1415	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	11	48	theme	kojibiose	2264:2272	arg1	resistance					2250:2259	the resistance	2246:2259	the resistance of kojibiose	2246:2272	While the resistance of kojibiose was established with monocultures, delayed utilization of kojibiose was observed with communities containing lactobacilli and A. viscosus as well as with complex communities of bacteria from human saliva.
32561577	10	49	contain	has	2041:2043	arg1	kojibiose					2031:2039	kojibiose	2031:2039	kojibiose	2031:2039	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	10	49	contain	has	2041:2043	arg2	properties.IMPORTANCE					2060:2080	low cariogenic properties.IMPORTANCE	2045:2080	low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible	2045:2237	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	0	50	theme	Differential	33:44	arg1	Kinetics					56:63	Oral Microbiota Display Profound Differential Metabolic Kinetics	0:63	Oral Microbiota Display Profound Differential Metabolic Kinetics	0:63	Oral Microbiota Display Profound Differential Metabolic Kinetics and Community Shifts upon Incubation with Sucrose, Trehalose, Kojibiose, and Xylitol.
32561577	13	51	theme	conventional	2570:2581	arg1	sugars					2583:2588	conventional sugars	2570:2588	conventional sugars	2570:2588	Moreover, although conventional sugars cause distinct shifts in salivary microbial communities, our study has revealed that kojibiose is able to closely maintain the salivary microbiome composition, suggesting its low cariogenic properties.
32561577	1	52	with	incubation	247:256	arg1	bacteria					283:290	representative oral bacteria	263:290	representative oral bacteria as monocultures or synthetic communities	263:331	This study compares the metabolic properties of kojibiose, trehalose, sucrose, and xylitol upon incubation with representative oral bacteria as monocultures or synthetic communities or with human salivary bacteria in a defined medium.
32561577	1	52	with	incubation	247:256	arg1	bacteria					356:363	human salivary bacteria	341:363	human salivary bacteria	341:363	This study compares the metabolic properties of kojibiose, trehalose, sucrose, and xylitol upon incubation with representative oral bacteria as monocultures or synthetic communities or with human salivary bacteria in a defined medium.
32561577	2	53	theme	48-h	460:463	arg1	incubation					465:474	a 48-h incubation	458:474	a 48-h incubation with monocultures	458:492	Compared to sucrose and trehalose, kojibiose resisted metabolism during a 48-h incubation with monocultures, except for Actinomyces viscosus Incubations with Lactobacillus-based communities, as well as salivary bacteria, displayed kojibiose metabolism, yet to a lesser extent than sucrose and trehalose.
32561577	14	54	theme	complex	3084:3090	arg1	interactions					3092:3103	complex interactions	3084:3103	complex interactions between oral microbiota	3084:3127	This study furthermore underscores the importance and relevance of microbial culture and ex vivo mixed cultures to study cariogenicity and substrate utilization; this is in sharp contrast with tests that solely rely on monocultures such as Streptococcus mutans, which clearly fail to capture complex interactions between oral microbiota.
32561577	10	55	from	insight	2124:2130	arg1	metabolism					2139:2148	the metabolism	2135:2148	the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible	2135:2237	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	1	56	theme	synthetic	311:319	arg1	communities					321:331	synthetic communities	311:331	synthetic communities	311:331	This study compares the metabolic properties of kojibiose, trehalose, sucrose, and xylitol upon incubation with representative oral bacteria as monocultures or synthetic communities or with human salivary bacteria in a defined medium.
32561577	10	57	theme	cariogenic	2049:2058	arg1	properties.IMPORTANCE					2060:2080	low cariogenic properties.IMPORTANCE	2045:2080	low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible	2045:2237	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	13	58	theme	low	2765:2767	arg1	properties					2780:2789	its low cariogenic properties	2761:2789	its low cariogenic properties	2761:2789	Moreover, although conventional sugars cause distinct shifts in salivary microbial communities, our study has revealed that kojibiose is able to closely maintain the salivary microbiome composition, suggesting its low cariogenic properties.
32561577	7	59	theme	α-diversity	1305:1315	arg1	markers					1317:1323	α-diversity markers	1305:1323	α-diversity markers	1305:1323	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	5	60	from	differences	968:978	arg1	composition					1003:1013	salivary microbiome composition	983:1013	salivary microbiome composition	983:1013	Donor-dependent differences in salivary microbiome composition were noted, and differences in pH drop during incubation indicated different rates of sugar metabolism.
32561577	11	61	from	lactobacilli	2383:2394	arg1	saliva					2471:2476	human saliva	2465:2476	human saliva	2465:2476	While the resistance of kojibiose was established with monocultures, delayed utilization of kojibiose was observed with communities containing lactobacilli and A. viscosus as well as with complex communities of bacteria from human saliva.
32561577	8	62	attach	linked	1504:1509	arg2	taxon					1498:1502	a taxon	1496:1502	a taxon linked to cariogenesis	1496:1525	In Streptococcus spp., a taxon linked to cariogenesis dominated in sucrose (mean ± standard deviation, 91.8 ± 6.4%) and trehalose (55.9 ± 38.6%), representing a high diversity loss.
32561577	8	62	attach	linked	1504:1509	arg1	cariogenesis					1514:1525	cariogenesis	1514:1525	cariogenesis	1514:1525	In Streptococcus spp., a taxon linked to cariogenesis dominated in sucrose (mean ± standard deviation, 91.8 ± 6.4%) and trehalose (55.9 ± 38.6%), representing a high diversity loss.
32561577	1	63	theme	xylitol	234:240	arg1	properties					185:194	the metabolic properties	171:194	the metabolic properties of kojibiose, trehalose, sucrose, and xylitol	171:240	This study compares the metabolic properties of kojibiose, trehalose, sucrose, and xylitol upon incubation with representative oral bacteria as monocultures or synthetic communities or with human salivary bacteria in a defined medium.
32561577	2	64	theme	kojibiose	617:625	arg1	metabolism					627:636	kojibiose metabolism	617:636	kojibiose metabolism	617:636	Compared to sucrose and trehalose, kojibiose resisted metabolism during a 48-h incubation with monocultures, except for Actinomyces viscosus Incubations with Lactobacillus-based communities, as well as salivary bacteria, displayed kojibiose metabolism, yet to a lesser extent than sucrose and trehalose.
32561577	7	65	theme	gene	1276:1279	arg1	analysis					1292:1299	16S rRNA gene sequencing analysis	1267:1299	16S rRNA gene sequencing analysis	1267:1299	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	11	66	theme	kojibiose	2332:2340	arg1	utilization					2317:2327	delayed utilization	2309:2327	delayed utilization of kojibiose	2309:2340	While the resistance of kojibiose was established with monocultures, delayed utilization of kojibiose was observed with communities containing lactobacilli and A. viscosus as well as with complex communities of bacteria from human saliva.
32561577	8	67	theme	diversity	1639:1647	arg1	loss					1649:1652	a high diversity loss	1632:1652	a high diversity loss	1632:1652	In Streptococcus spp., a taxon linked to cariogenesis dominated in sucrose (mean ± standard deviation, 91.8 ± 6.4%) and trehalose (55.9 ± 38.6%), representing a high diversity loss.
32561577	6	68	theme	metabolic	1214:1222	arg1	profile					1224:1230	the metabolic profile	1210:1230	the metabolic profile for all sugars	1210:1245	However, functional analysis indicated that lactate, acetate, and formate evenly dominated the metabolic profile for all sugars except for xylitol.
32561577	13	69	theme	salivary	2717:2724	arg1	composition					2737:2747	the salivary microbiome composition	2713:2747	the salivary microbiome composition	2713:2747	Moreover, although conventional sugars cause distinct shifts in salivary microbial communities, our study has revealed that kojibiose is able to closely maintain the salivary microbiome composition, suggesting its low cariogenic properties.
32561577	14	70	theme	sharp	2965:2969	arg1	contrast					2971:2978	sharp contrast	2965:2978	sharp contrast with tests that solely rely on monocultures such as Streptococcus mutans, which clearly fail to capture complex interactions between oral microbiota	2965:3127	This study furthermore underscores the importance and relevance of microbial culture and ex vivo mixed cultures to study cariogenicity and substrate utilization; this is in sharp contrast with tests that solely rely on monocultures such as Streptococcus mutans, which clearly fail to capture complex interactions between oral microbiota.
32561577	1	71	theme	metabolic	175:183	arg1	properties					185:194	the metabolic properties	171:194	the metabolic properties of kojibiose, trehalose, sucrose, and xylitol	171:240	This study compares the metabolic properties of kojibiose, trehalose, sucrose, and xylitol upon incubation with representative oral bacteria as monocultures or synthetic communities or with human salivary bacteria in a defined medium.
32561577	4	72	theme	GH	862:863	arg1	families					866:873	glycohydrolase (GH) families GH65 and GH15	846:887	glycohydrolase (GH) families GH65 and GH15	846:887	and A. viscosus possess enzymes from glycohydrolase (GH) families GH65 and GH15, respectively, which are associated with kojibiose metabolism.
32561577	4	72	theme	GH	862:863	arg1	GH15					884:887	GH15	884:887	GH15	884:887	and A. viscosus possess enzymes from glycohydrolase (GH) families GH65 and GH15, respectively, which are associated with kojibiose metabolism.
32561577	4	72	theme	GH	862:863	arg1	GH65					875:878	GH65	875:878	GH65	875:878	and A. viscosus possess enzymes from glycohydrolase (GH) families GH65 and GH15, respectively, which are associated with kojibiose metabolism.
32561577	10	73	theme	detailed	2104:2111	arg1	insight					2124:2130	a detailed scientific insight	2102:2130	a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible	2102:2237	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	10	74	theme	cariogenic	1903:1912	arg1	species					1914:1920	cariogenic species	1903:1920	cariogenic species	1903:1920	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	3	75	with	Concurring	690:699	arg1	findings					719:726	our in vitro findings	706:726	our in vitro findings	706:726	Concurring with our in vitro findings, screening for carbohydrate-active enzymes revealed that only Lactobacillus spp.
32561577	4	76	dep	A.	813:814	arg1	viscosus					816:823	and A. viscosus	809:823	viscosus	816:823	and A. viscosus possess enzymes from glycohydrolase (GH) families GH65 and GH15, respectively, which are associated with kojibiose metabolism.
32561577	14	77	theme	microbial	2859:2867	arg1	culture					2869:2875	microbial culture	2859:2875	microbial culture	2859:2875	This study furthermore underscores the importance and relevance of microbial culture and ex vivo mixed cultures to study cariogenicity and substrate utilization; this is in sharp contrast with tests that solely rely on monocultures such as Streptococcus mutans, which clearly fail to capture complex interactions between oral microbiota.
32561577	1	78	from	incubation	247:256	arg1	medium					378:383	a defined medium	368:383	a defined medium	368:383	This study compares the metabolic properties of kojibiose, trehalose, sucrose, and xylitol upon incubation with representative oral bacteria as monocultures or synthetic communities or with human salivary bacteria in a defined medium.
32561577	14	79	theme	substrate	2931:2939	arg1	utilization					2941:2951	substrate utilization	2931:2951	substrate utilization	2931:2951	This study furthermore underscores the importance and relevance of microbial culture and ex vivo mixed cultures to study cariogenicity and substrate utilization; this is in sharp contrast with tests that solely rely on monocultures such as Streptococcus mutans, which clearly fail to capture complex interactions between oral microbiota.
32561577	5	80	theme	metabolism	1107:1116	arg1	rates					1092:1096	different rates	1082:1096	different rates of sugar metabolism	1082:1116	Donor-dependent differences in salivary microbiome composition were noted, and differences in pH drop during incubation indicated different rates of sugar metabolism.
32561577	2	81	theme	Lactobacillus-based	544:562	arg1	communities					564:574	Lactobacillus-based communities	544:574	Lactobacillus-based communities	544:574	Compared to sucrose and trehalose, kojibiose resisted metabolism during a 48-h incubation with monocultures, except for Actinomyces viscosus Incubations with Lactobacillus-based communities, as well as salivary bacteria, displayed kojibiose metabolism, yet to a lesser extent than sucrose and trehalose.
32561577	11	82	theme	complex	2428:2434	arg1	communities					2436:2446	complex communities	2428:2446	complex communities of bacteria from human saliva	2428:2476	While the resistance of kojibiose was established with monocultures, delayed utilization of kojibiose was observed with communities containing lactobacilli and A. viscosus as well as with complex communities of bacteria from human saliva.
32561577	10	83	theme	phyla	1970:1974	arg1	genera					1980:1985	genera	1980:1985	genera of the salivary microbiome	1980:2012	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	10	83	theme	phyla	1970:1974	arg1	abundance					1953:1961	an abundance	1950:1961	an abundance of key phyla	1950:1974	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	14	84	with	contrast	2971:2978	arg1	tests					2985:2989	tests	2985:2989	tests that solely rely on monocultures such as Streptococcus mutans, which clearly fail to capture complex interactions between oral microbiota	2985:3127	This study furthermore underscores the importance and relevance of microbial culture and ex vivo mixed cultures to study cariogenicity and substrate utilization; this is in sharp contrast with tests that solely rely on monocultures such as Streptococcus mutans, which clearly fail to capture complex interactions between oral microbiota.
32561577	9	85	from	contrast	1658:1665	arg1	abundant					1704:1711	abundant	1704:1711	abundant	1704:1711	In contrast, Streptococcus (5.1 ± 3.7%) was less abundant in kojibiose, which instead was dominated by Veillonella (26.8 ± 19.6%), while for xylitol, Neisseria (29.4 ± 19.1%) was most abundant.
32561577	2	86	theme	salivary	588:595	arg1	bacteria					597:604	salivary bacteria	588:604	salivary bacteria	588:604	Compared to sucrose and trehalose, kojibiose resisted metabolism during a 48-h incubation with monocultures, except for Actinomyces viscosus Incubations with Lactobacillus-based communities, as well as salivary bacteria, displayed kojibiose metabolism, yet to a lesser extent than sucrose and trehalose.
32561577	10	87	theme	rare	2155:2158	arg1	disaccharide					2160:2171	a rare disaccharide	2153:2171	a rare disaccharide	2153:2171	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	10	87	theme	rare	2155:2158	arg1	kojibiose					2174:2182	kojibiose	2174:2182	kojibiose	2174:2182	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	5	88	theme	different	1082:1090	arg1	rates					1092:1096	different rates	1082:1096	different rates of sugar metabolism	1082:1116	Donor-dependent differences in salivary microbiome composition were noted, and differences in pH drop during incubation indicated different rates of sugar metabolism.
32561577	10	89	theme	mass	2191:2194	arg1	production					2196:2205	production	2196:2205	production	2196:2205	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	4	90	contain	possess	825:831	arg1	A.					813:814	A.	813:814	A.	813:814	and A. viscosus possess enzymes from glycohydrolase (GH) families GH65 and GH15, respectively, which are associated with kojibiose metabolism.
32561577	4	90	contain	possess	825:831	arg2	enzymes					833:839	enzymes	833:839	enzymes	833:839	and A. viscosus possess enzymes from glycohydrolase (GH) families GH65 and GH15, respectively, which are associated with kojibiose metabolism.
32561577	7	91	from	kojibiose	1450:1458	arg1	shift					1353:1357	a significant shift	1339:1357	a significant shift of the microbiome community by sugars	1339:1395	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	7	91	from	kojibiose	1450:1458	arg1	pronounced					1406:1415	pronounced	1406:1415	pronounced	1406:1415	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	2	92	with	incubation	465:474	arg1	monocultures					481:492	monocultures	481:492	monocultures	481:492	Compared to sucrose and trehalose, kojibiose resisted metabolism during a 48-h incubation with monocultures, except for Actinomyces viscosus Incubations with Lactobacillus-based communities, as well as salivary bacteria, displayed kojibiose metabolism, yet to a lesser extent than sucrose and trehalose.
32561577	7	93	from	xylitol	1464:1470	arg1	shift					1353:1357	a significant shift	1339:1357	a significant shift of the microbiome community by sugars	1339:1395	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	7	93	from	xylitol	1464:1470	arg1	pronounced					1406:1415	pronounced	1406:1415	pronounced	1406:1415	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	13	94	theme	microbial	2624:2632	arg1	communities					2634:2644	salivary microbial communities	2615:2644	salivary microbial communities	2615:2644	Moreover, although conventional sugars cause distinct shifts in salivary microbial communities, our study has revealed that kojibiose is able to closely maintain the salivary microbiome composition, suggesting its low cariogenic properties.
32561577	1	95	theme	salivary	347:354	arg1	bacteria					356:363	human salivary bacteria	341:363	human salivary bacteria	341:363	This study compares the metabolic properties of kojibiose, trehalose, sucrose, and xylitol upon incubation with representative oral bacteria as monocultures or synthetic communities or with human salivary bacteria in a defined medium.
32561577	5	96	theme	microbiome	992:1001	arg1	composition					1003:1013	salivary microbiome composition	983:1013	salivary microbiome composition	983:1013	Donor-dependent differences in salivary microbiome composition were noted, and differences in pH drop during incubation indicated different rates of sugar metabolism.
32561577	10	97	theme	microbiome	2003:2012	arg1	genera					1980:1985	genera	1980:1985	genera of the salivary microbiome	1980:2012	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	10	97	theme	microbiome	2003:2012	arg1	abundance					1953:1961	an abundance	1950:1961	an abundance of key phyla	1950:1974	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	4	98	from	families	866:873	arg1	enzymes					833:839	enzymes	833:839	enzymes	833:839	and A. viscosus possess enzymes from glycohydrolase (GH) families GH65 and GH15, respectively, which are associated with kojibiose metabolism.
32561577	7	99	theme	microbiome	1366:1375	arg1	community					1377:1385	the microbiome community	1362:1385	the microbiome community by sugars	1362:1395	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	2	100	theme	Actinomyces	506:516	arg1	Incubations					527:537	Actinomyces viscosus Incubations	506:537	Actinomyces viscosus Incubations	506:537	Compared to sucrose and trehalose, kojibiose resisted metabolism during a 48-h incubation with monocultures, except for Actinomyces viscosus Incubations with Lactobacillus-based communities, as well as salivary bacteria, displayed kojibiose metabolism, yet to a lesser extent than sucrose and trehalose.
32561577	0	101	theme	Microbiota	5:14	arg1	Kinetics					56:63	Oral Microbiota Display Profound Differential Metabolic Kinetics	0:63	Oral Microbiota Display Profound Differential Metabolic Kinetics	0:63	Oral Microbiota Display Profound Differential Metabolic Kinetics and Community Shifts upon Incubation with Sucrose, Trehalose, Kojibiose, and Xylitol.
32561577	1	102	theme	defined	370:376	arg1	medium					378:383	a defined medium	368:383	a defined medium	368:383	This study compares the metabolic properties of kojibiose, trehalose, sucrose, and xylitol upon incubation with representative oral bacteria as monocultures or synthetic communities or with human salivary bacteria in a defined medium.
32561577	5	103	theme	Donor-dependent	952:966	arg1	differences					968:978	Donor-dependent differences	952:978	Donor-dependent differences in salivary microbiome composition	952:1013	Donor-dependent differences in salivary microbiome composition were noted, and differences in pH drop during incubation indicated different rates of sugar metabolism.
32561577	0	104	theme	Profound	24:31	arg1	Kinetics					56:63	Oral Microbiota Display Profound Differential Metabolic Kinetics	0:63	Oral Microbiota Display Profound Differential Metabolic Kinetics	0:63	Oral Microbiota Display Profound Differential Metabolic Kinetics and Community Shifts upon Incubation with Sucrose, Trehalose, Kojibiose, and Xylitol.
32561577	1	105	theme	oral	278:281	arg1	bacteria					283:290	representative oral bacteria	263:290	representative oral bacteria as monocultures or synthetic communities	263:331	This study compares the metabolic properties of kojibiose, trehalose, sucrose, and xylitol upon incubation with representative oral bacteria as monocultures or synthetic communities or with human salivary bacteria in a defined medium.
32561577	2	106	with	Incubations	527:537	arg1	communities					564:574	Lactobacillus-based communities	544:574	Lactobacillus-based communities	544:574	Compared to sucrose and trehalose, kojibiose resisted metabolism during a 48-h incubation with monocultures, except for Actinomyces viscosus Incubations with Lactobacillus-based communities, as well as salivary bacteria, displayed kojibiose metabolism, yet to a lesser extent than sucrose and trehalose.
32561577	7	107	theme	significant	1341:1351	arg1	shift					1353:1357	a significant shift	1339:1357	a significant shift of the microbiome community by sugars	1339:1395	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	7	107	theme	significant	1341:1351	arg1	pronounced					1406:1415	pronounced	1406:1415	pronounced	1406:1415	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	0	108	theme	Metabolic	46:54	arg1	Kinetics					56:63	Oral Microbiota Display Profound Differential Metabolic Kinetics	0:63	Oral Microbiota Display Profound Differential Metabolic Kinetics	0:63	Oral Microbiota Display Profound Differential Metabolic Kinetics and Community Shifts upon Incubation with Sucrose, Trehalose, Kojibiose, and Xylitol.
32561577	5	109	theme	pH	1046:1047	arg1	drop					1049:1052	pH drop	1046:1052	pH drop during incubation	1046:1070	Donor-dependent differences in salivary microbiome composition were noted, and differences in pH drop during incubation indicated different rates of sugar metabolism.
32561577	11	110	from	communities	2436:2446	arg1	saliva					2471:2476	human saliva	2465:2476	human saliva	2465:2476	While the resistance of kojibiose was established with monocultures, delayed utilization of kojibiose was observed with communities containing lactobacilli and A. viscosus as well as with complex communities of bacteria from human saliva.
32561577	10	111	theme	kojibiose	1858:1866	arg1	incubations					1880:1890	kojibiose and xylitol incubations	1858:1890	kojibiose and xylitol incubations	1858:1890	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	4	112	theme	glycohydrolase	846:859	arg1	families					866:873	glycohydrolase (GH) families GH65 and GH15	846:887	glycohydrolase (GH) families GH65 and GH15	846:887	and A. viscosus possess enzymes from glycohydrolase (GH) families GH65 and GH15, respectively, which are associated with kojibiose metabolism.
32561577	4	112	theme	glycohydrolase	846:859	arg1	GH15					884:887	GH15	884:887	GH15	884:887	and A. viscosus possess enzymes from glycohydrolase (GH) families GH65 and GH15, respectively, which are associated with kojibiose metabolism.
32561577	4	112	theme	glycohydrolase	846:859	arg1	GH65					875:878	GH65	875:878	GH65	875:878	and A. viscosus possess enzymes from glycohydrolase (GH) families GH65 and GH15, respectively, which are associated with kojibiose metabolism.
32561577	10	113	theme	low	2045:2047	arg1	properties.IMPORTANCE					2060:2080	low cariogenic properties.IMPORTANCE	2045:2080	low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible	2045:2237	Overall, kojibiose and xylitol incubations stimulated cariogenic species less yet closely maintained an abundance of key phyla and genera of the salivary microbiome, suggesting that kojibiose has low cariogenic properties.IMPORTANCE This study provides a detailed scientific insight on the metabolism of a rare disaccharide, kojibiose, whose mass production has recently been made possible.
32561577	13	114	theme	cariogenic	2769:2778	arg1	properties					2780:2789	its low cariogenic properties	2761:2789	its low cariogenic properties	2761:2789	Moreover, although conventional sugars cause distinct shifts in salivary microbial communities, our study has revealed that kojibiose is able to closely maintain the salivary microbiome composition, suggesting its low cariogenic properties.
32561577	1	115	theme	sucrose	221:227	arg1	properties					185:194	the metabolic properties	171:194	the metabolic properties of kojibiose, trehalose, sucrose, and xylitol	171:240	This study compares the metabolic properties of kojibiose, trehalose, sucrose, and xylitol upon incubation with representative oral bacteria as monocultures or synthetic communities or with human salivary bacteria in a defined medium.
32561577	7	116	theme	sequencing	1281:1290	arg1	analysis					1292:1299	16S rRNA gene sequencing analysis	1267:1299	16S rRNA gene sequencing analysis	1267:1299	16S rRNA gene sequencing analysis and α-diversity markers revealed that a significant shift of the microbiome community by sugars was more pronounced in sucrose and trehalose than in kojibiose and xylitol.
32561577	11	117	theme	delayed	2309:2315	arg1	utilization					2317:2327	delayed utilization	2309:2327	delayed utilization of kojibiose	2309:2340	While the resistance of kojibiose was established with monocultures, delayed utilization of kojibiose was observed with communities containing lactobacilli and A. viscosus as well as with complex communities of bacteria from human saliva.
32561577	8	118	theme	high	1634:1637	arg1	diversity					1639:1647	a high diversity	1632:1647	a high diversity loss	1632:1652	In Streptococcus spp., a taxon linked to cariogenesis dominated in sucrose (mean ± standard deviation, 91.8 ± 6.4%) and trehalose (55.9 ± 38.6%), representing a high diversity loss.
32561577	3	119	theme	in	710:711	arg1	findings					719:726	our in vitro findings	706:726	our in vitro findings	706:726	Concurring with our in vitro findings, screening for carbohydrate-active enzymes revealed that only Lactobacillus spp.
32561577	14	120	dep	importance	2831:2840	arg1	the					2827:2829	the	2827:2829	the	2827:2829	This study furthermore underscores the importance and relevance of microbial culture and ex vivo mixed cultures to study cariogenicity and substrate utilization; this is in sharp contrast with tests that solely rely on monocultures such as Streptococcus mutans, which clearly fail to capture complex interactions between oral microbiota.
32561577	13	121	theme	distinct	2596:2603	arg1	shifts					2605:2610	distinct shifts	2596:2610	distinct shifts in salivary microbial communities	2596:2644	Moreover, although conventional sugars cause distinct shifts in salivary microbial communities, our study has revealed that kojibiose is able to closely maintain the salivary microbiome composition, suggesting its low cariogenic properties.
32561577	13	122	theme	microbiome	2726:2735	arg1	composition					2737:2747	the salivary microbiome composition	2713:2747	the salivary microbiome composition	2713:2747	Moreover, although conventional sugars cause distinct shifts in salivary microbial communities, our study has revealed that kojibiose is able to closely maintain the salivary microbiome composition, suggesting its low cariogenic properties.
32936806	4	0	theme	manufacturing	661:673	arg1	field					630:634	the field	626:634	the field of bioinspired chitinous manufacturing	626:673	Here, we make use of recent advances in the field of bioinspired chitinous manufacturing to develop a manufacturing technology to be used within the context of a minimal, artificial ecosystem that supports humans in a Martian environment.
32936806	1	1	theme	critical	213:220	arg1	technologies					222:233	critical technologies	213:233	critical technologies	213:233	Given plans to revisit the lunar surface by the late 2020s and to take a crewed mission to Mars by the late 2030s, critical technologies must mature.
32936806	4	2	theme	chitinous	651:659	arg1	manufacturing					661:673	bioinspired chitinous manufacturing	639:673	bioinspired chitinous manufacturing	639:673	Here, we make use of recent advances in the field of bioinspired chitinous manufacturing to develop a manufacturing technology to be used within the context of a minimal, artificial ecosystem that supports humans in a Martian environment.
32936806	3	3	theme	increasing	504:513	arg1	need					515:518	the increasing need to develop resource-efficient and zero-waste ecosystems on Earth	500:583	the increasing need to develop resource-efficient and zero-waste ecosystems on Earth	500:583	While this present a significantly more complex challenge in the resource-starved environment of Mars, it is similar to the increasing need to develop resource-efficient and zero-waste ecosystems on Earth.
32936806	3	4	theme	resource-efficient	531:548	arg1	ecosystems					565:574	resource-efficient and zero-waste ecosystems	531:574	resource-efficient and zero-waste ecosystems on Earth	531:583	While this present a significantly more complex challenge in the resource-starved environment of Mars, it is similar to the increasing need to develop resource-efficient and zero-waste ecosystems on Earth.
32936806	4	5	theme	manufacturing	688:700	arg1	technology					702:711	a manufacturing technology	686:711	a manufacturing technology to be used within the context of a minimal, artificial ecosystem that supports humans in a Martian environment	686:822	Here, we make use of recent advances in the field of bioinspired chitinous manufacturing to develop a manufacturing technology to be used within the context of a minimal, artificial ecosystem that supports humans in a Martian environment.
32936806	4	6	from	field	630:634	arg1	use					600:602	use	600:602	use of recent advances in the field of bioinspired chitinous manufacturing to develop a manufacturing technology to be used within the context of a minimal, artificial ecosystem that supports humans in a Martian environment	600:822	Here, we make use of recent advances in the field of bioinspired chitinous manufacturing to develop a manufacturing technology to be used within the context of a minimal, artificial ecosystem that supports humans in a Martian environment.
32936806	3	7	theme	more	415:418	arg1	challenge					428:436	a significantly more complex challenge	399:436	a significantly more complex challenge in the resource-starved environment of Mars	399:480	While this present a significantly more complex challenge in the resource-starved environment of Mars, it is similar to the increasing need to develop resource-efficient and zero-waste ecosystems on Earth.
32936806	2	8	dep	in	282:283	arg1	situ					285:288	situ	285:288	situ	285:288	In missions of extended duration, in situ resource utilization is necessary to both maximize scientific returns and minimize costs.
32936806	2	9	from	necessary	314:322	arg1	missions					251:258	missions	251:258	missions of extended duration	251:279	In missions of extended duration, in situ resource utilization is necessary to both maximize scientific returns and minimize costs.
32936806	2	10	theme	in	282:283	arg1	utilization					299:309	in situ resource utilization	282:309	in situ resource utilization	282:309	In missions of extended duration, in situ resource utilization is necessary to both maximize scientific returns and minimize costs.
32936806	4	11	from	advances	614:621	arg1	field					630:634	the field	626:634	the field of bioinspired chitinous manufacturing	626:673	Here, we make use of recent advances in the field of bioinspired chitinous manufacturing to develop a manufacturing technology to be used within the context of a minimal, artificial ecosystem that supports humans in a Martian environment.
32936806	4	12	dep	minimal	748:754	arg1	artificial					757:766	artificial	757:766	artificial	757:766	Here, we make use of recent advances in the field of bioinspired chitinous manufacturing to develop a manufacturing technology to be used within the context of a minimal, artificial ecosystem that supports humans in a Martian environment.
32936806	0	13	theme	Martian	0:6	arg1	biolith					8:14	Martian biolith	0:14	Martian biolith: A bioinspired regolith composite for closed-loop extraterrestrial manufacturing.	0:96	Martian biolith: A bioinspired regolith composite for closed-loop extraterrestrial manufacturing.
32936806	4	14	theme	Martian	804:810	arg1	environment					812:822	a Martian environment	802:822	a Martian environment	802:822	Here, we make use of recent advances in the field of bioinspired chitinous manufacturing to develop a manufacturing technology to be used within the context of a minimal, artificial ecosystem that supports humans in a Martian environment.
32936806	1	15	theme	crewed	171:176	arg1	mission					178:184	a crewed mission	169:184	a crewed mission to Mars	169:192	Given plans to revisit the lunar surface by the late 2020s and to take a crewed mission to Mars by the late 2030s, critical technologies must mature.
32936806	2	16	theme	duration	272:279	arg1	missions					251:258	missions	251:258	missions of extended duration	251:279	In missions of extended duration, in situ resource utilization is necessary to both maximize scientific returns and minimize costs.
32936806	3	17	from	ecosystems	565:574	arg1	Earth					579:583	Earth	579:583	Earth	579:583	While this present a significantly more complex challenge in the resource-starved environment of Mars, it is similar to the increasing need to develop resource-efficient and zero-waste ecosystems on Earth.
32936806	2	18	theme	extended	263:270	arg1	duration					272:279	extended duration	263:279	extended duration	263:279	In missions of extended duration, in situ resource utilization is necessary to both maximize scientific returns and minimize costs.
32936806	4	19	theme	advances	614:621	arg1	use					600:602	use	600:602	use of recent advances in the field of bioinspired chitinous manufacturing to develop a manufacturing technology to be used within the context of a minimal, artificial ecosystem that supports humans in a Martian environment	600:822	Here, we make use of recent advances in the field of bioinspired chitinous manufacturing to develop a manufacturing technology to be used within the context of a minimal, artificial ecosystem that supports humans in a Martian environment.
32936806	0	20	theme	regolith	31:38	arg1	composite					40:48	A bioinspired regolith composite	17:48	Martian biolith: A bioinspired regolith composite for closed-loop extraterrestrial manufacturing.	0:96	Martian biolith: A bioinspired regolith composite for closed-loop extraterrestrial manufacturing.
32936806	3	21	theme	Mars	477:480	arg1	environment					462:472	the resource-starved environment	441:472	the resource-starved environment of Mars	441:480	While this present a significantly more complex challenge in the resource-starved environment of Mars, it is similar to the increasing need to develop resource-efficient and zero-waste ecosystems on Earth.
32936806	4	22	theme	recent	607:612	arg1	advances					614:621	recent advances	607:621	recent advances in the field of bioinspired chitinous manufacturing	607:673	Here, we make use of recent advances in the field of bioinspired chitinous manufacturing to develop a manufacturing technology to be used within the context of a minimal, artificial ecosystem that supports humans in a Martian environment.
32936806	3	23	theme	complex	420:426	arg1	challenge					428:436	a significantly more complex challenge	399:436	a significantly more complex challenge in the resource-starved environment of Mars	399:480	While this present a significantly more complex challenge in the resource-starved environment of Mars, it is similar to the increasing need to develop resource-efficient and zero-waste ecosystems on Earth.
32936806	0	24	theme	bioinspired	19:29	arg1	composite					40:48	A bioinspired regolith composite	17:48	Martian biolith: A bioinspired regolith composite for closed-loop extraterrestrial manufacturing.	0:96	Martian biolith: A bioinspired regolith composite for closed-loop extraterrestrial manufacturing.
32936806	4	25	theme	ecosystem	768:776	arg1	context					735:741	the context	731:741	the context of a minimal, artificial ecosystem that supports humans in a Martian environment	731:822	Here, we make use of recent advances in the field of bioinspired chitinous manufacturing to develop a manufacturing technology to be used within the context of a minimal, artificial ecosystem that supports humans in a Martian environment.
32936806	3	26	theme	zero-waste	554:563	arg1	ecosystems					565:574	resource-efficient and zero-waste ecosystems	531:574	resource-efficient and zero-waste ecosystems on Earth	531:583	While this present a significantly more complex challenge in the resource-starved environment of Mars, it is similar to the increasing need to develop resource-efficient and zero-waste ecosystems on Earth.
32936806	3	27	from	challenge	428:436	arg1	environment					462:472	the resource-starved environment	441:472	the resource-starved environment of Mars	441:480	While this present a significantly more complex challenge in the resource-starved environment of Mars, it is similar to the increasing need to develop resource-efficient and zero-waste ecosystems on Earth.
32936806	3	28	theme	resource-starved	445:460	arg1	environment					462:472	the resource-starved environment	441:472	the resource-starved environment of Mars	441:480	While this present a significantly more complex challenge in the resource-starved environment of Mars, it is similar to the increasing need to develop resource-efficient and zero-waste ecosystems on Earth.
32936806	4	29	from	use	600:602	arg1	field					630:634	the field	626:634	the field of bioinspired chitinous manufacturing	626:673	Here, we make use of recent advances in the field of bioinspired chitinous manufacturing to develop a manufacturing technology to be used within the context of a minimal, artificial ecosystem that supports humans in a Martian environment.
32936806	1	30	theme	lunar	125:129	arg1	surface					131:137	the lunar surface	121:137	the lunar surface	121:137	Given plans to revisit the lunar surface by the late 2020s and to take a crewed mission to Mars by the late 2030s, critical technologies must mature.
32936806	4	31	theme	minimal	748:754	arg1	ecosystem					768:776	a minimal, artificial ecosystem	746:776	a minimal, artificial ecosystem that supports humans in a Martian environment	746:822	Here, we make use of recent advances in the field of bioinspired chitinous manufacturing to develop a manufacturing technology to be used within the context of a minimal, artificial ecosystem that supports humans in a Martian environment.
32936806	2	32	theme	resource	290:297	arg1	utilization					299:309	in situ resource utilization	282:309	in situ resource utilization	282:309	In missions of extended duration, in situ resource utilization is necessary to both maximize scientific returns and minimize costs.
32936806	2	33	dep	scientific	341:350	arg1	returns					352:358	returns	352:358	returns	352:358	In missions of extended duration, in situ resource utilization is necessary to both maximize scientific returns and minimize costs.
32936806	1	34	theme	late	201:204	arg1	2030s					206:210	the late 2030s	197:210	the late 2030s	197:210	Given plans to revisit the lunar surface by the late 2020s and to take a crewed mission to Mars by the late 2030s, critical technologies must mature.
32936806	0	35	theme	extraterrestrial	66:81	arg1	manufacturing					83:95	closed-loop extraterrestrial manufacturing	54:95	closed-loop extraterrestrial manufacturing	54:95	Martian biolith: A bioinspired regolith composite for closed-loop extraterrestrial manufacturing.
32936806	0	36	dep	biolith	8:14	arg1	composite					40:48	A bioinspired regolith composite	17:48	Martian biolith: A bioinspired regolith composite for closed-loop extraterrestrial manufacturing.	0:96	Martian biolith: A bioinspired regolith composite for closed-loop extraterrestrial manufacturing.
32936806	4	37	theme	bioinspired	639:649	arg1	manufacturing					661:673	bioinspired chitinous manufacturing	639:673	bioinspired chitinous manufacturing	639:673	Here, we make use of recent advances in the field of bioinspired chitinous manufacturing to develop a manufacturing technology to be used within the context of a minimal, artificial ecosystem that supports humans in a Martian environment.
32936806	2	38	from	missions	251:258	arg1	necessary					314:322	necessary	314:322	necessary	314:322	In missions of extended duration, in situ resource utilization is necessary to both maximize scientific returns and minimize costs.
32936806	0	39	theme	closed-loop	54:64	arg1	manufacturing					83:95	closed-loop extraterrestrial manufacturing	54:95	closed-loop extraterrestrial manufacturing	54:95	Martian biolith: A bioinspired regolith composite for closed-loop extraterrestrial manufacturing.
32936806	1	40	theme	late	146:149	arg1	2020s					151:155	the late 2020s	142:155	the late 2020s	142:155	Given plans to revisit the lunar surface by the late 2020s and to take a crewed mission to Mars by the late 2030s, critical technologies must mature.
33246777	3	0	theme	transition	889:898	arg1	Ca2p1					876:880	Ca2p1	876:880	Ca2p1	876:880	XPS analysis displayed the presence of SiNPs (Si2p peak for silicon) and calcium (Ca2p1, Ca2p3 transition peaks) in the composite.
33246777	3	0	theme	transition	889:898	arg1	peaks					900:904	Ca2p3 transition peaks	883:904	Ca2p3 transition peaks	883:904	XPS analysis displayed the presence of SiNPs (Si2p peak for silicon) and calcium (Ca2p1, Ca2p3 transition peaks) in the composite.
33246777	4	1	theme	percolation	950:960	arg1	simulation					962:971	in silico percolation simulation	940:971	in silico percolation simulation for composite	940:985	Interestingly, in silico percolation simulation for composite revealed interlinked 800 μm long-conduits predicting excellent absorption capacity and validated experimentally (640 % of composite dry weight).
33246777	7	2	theme	compressive	1406:1416	arg1	2.45 MPa					1428:1435	2.45 MPa	1428:1435	2.45 MPa	1428:1435	It displayed high compressive strength (2.45 MPa) and withstood pressure during application.
33246777	7	2	theme	compressive	1406:1416	arg1	strength					1418:1425	high compressive strength	1401:1425	high compressive strength (2.45 MPa)	1401:1436	It displayed high compressive strength (2.45 MPa) and withstood pressure during application.
33246777	4	3	link	interlinked	996:1006	arg1	800 μm long-conduits					1008:1027	interlinked 800 μm long-conduits	996:1027	interlinked 800 μm long-conduits predicting excellent absorption capacity and validated experimentally (640 % of composite dry weight)	996:1129	Interestingly, in silico percolation simulation for composite revealed interlinked 800 μm long-conduits predicting excellent absorption capacity and validated experimentally (640 % of composite dry weight).
33246777	3	4	dep	calcium	867:873	arg1	Ca2p1					876:880	Ca2p1	876:880	Ca2p1	876:880	XPS analysis displayed the presence of SiNPs (Si2p peak for silicon) and calcium (Ca2p1, Ca2p3 transition peaks) in the composite.
33246777	3	4	dep	calcium	867:873	arg1	peaks					900:904	Ca2p3 transition peaks	883:904	Ca2p3 transition peaks	883:904	XPS analysis displayed the presence of SiNPs (Si2p peak for silicon) and calcium (Ca2p1, Ca2p3 transition peaks) in the composite.
33246777	5	5	theme	improved	1164:1171	arg1	clotting					1179:1186	>16-fold improved blood clotting	1155:1186	>16-fold improved blood clotting	1155:1186	The composite achieved >16-fold improved blood clotting in vitro than commercial Celox and Gauze through multimodal interaction of its components with RBCs and platelets.
33246777	2	6	theme	silica	609:614	arg1	nanoparticles					616:628	0.5 mg synthesized silica nanoparticles	590:628	0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge)	590:664	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	3	7	from	presence	821:828	arg1	composite					914:922	composite	914:922	composite	914:922	XPS analysis displayed the presence of SiNPs (Si2p peak for silicon) and calcium (Ca2p1, Ca2p3 transition peaks) in the composite.
33246777	4	8	dep	in	940:941	arg1	silico					943:948	silico	943:948	silico	943:948	Interestingly, in silico percolation simulation for composite revealed interlinked 800 μm long-conduits predicting excellent absorption capacity and validated experimentally (640 % of composite dry weight).
33246777	2	9	theme	0.5 mg	590:595	arg1	nanoparticles					616:628	0.5 mg synthesized silica nanoparticles	590:628	0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge)	590:664	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	7	10	theme	withstood	1442:1450	arg1	pressure					1452:1459	withstood pressure	1442:1459	withstood pressure	1442:1459	It displayed high compressive strength (2.45 MPa) and withstood pressure during application.
33246777	2	11	theme	multimodal	311:320	arg1	hemostat					330:337	an efficient multimodal topical hemostat	298:337	an efficient multimodal topical hemostat	298:337	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	4	12	theme	excellent	1040:1048	arg1	capacity					1061:1068	excellent absorption capacity	1040:1068	excellent absorption capacity	1040:1068	Interestingly, in silico percolation simulation for composite revealed interlinked 800 μm long-conduits predicting excellent absorption capacity and validated experimentally (640 % of composite dry weight).
33246777	0	13	theme	chitosan-gelatin	89:104	arg1	composite					114:122	A multimodal chitosan-gelatin xerogel composite	76:122	Design and synthesis of a new topical agent for halting blood loss rapidly: A multimodal chitosan-gelatin xerogel composite loaded with silica nanoparticles and calcium.	0:168	Design and synthesis of a new topical agent for halting blood loss rapidly: A multimodal chitosan-gelatin xerogel composite loaded with silica nanoparticles and calcium.
33246777	2	14	theme	FTIR	728:731	arg1	analysis					733:740	FTIR analysis	728:740	FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively	728:791	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	3	15	attach	presence	821:828	arg1	composite					914:922	composite	914:922	composite	914:922	XPS analysis displayed the presence of SiNPs (Si2p peak for silicon) and calcium (Ca2p1, Ca2p3 transition peaks) in the composite.
33246777	3	15	attach	presence	821:828	arg2	SiNPs					833:837	SiNPs	833:837	SiNPs (Si2p peak for silicon)	833:861	XPS analysis displayed the presence of SiNPs (Si2p peak for silicon) and calcium (Ca2p1, Ca2p3 transition peaks) in the composite.
33246777	3	15	attach	presence	821:828	arg2	calcium					867:873	calcium	867:873	calcium (Ca2p1, Ca2p3 transition peaks)	867:905	XPS analysis displayed the presence of SiNPs (Si2p peak for silicon) and calcium (Ca2p1, Ca2p3 transition peaks) in the composite.
33246777	3	16	dep	SiNPs	833:837	arg1	peak					845:848	Si2p peak	840:848	Si2p peak	840:848	XPS analysis displayed the presence of SiNPs (Si2p peak for silicon) and calcium (Ca2p1, Ca2p3 transition peaks) in the composite.
33246777	4	17	theme	weight	1123:1128	arg1	%					1104:1104	640 %	1100:1104	640 % of composite dry weight	1100:1128	Interestingly, in silico percolation simulation for composite revealed interlinked 800 μm long-conduits predicting excellent absorption capacity and validated experimentally (640 % of composite dry weight).
33246777	4	17	theme	weight	1123:1128	arg1	weight					1123:1128	composite dry weight	1109:1128	composite dry weight	1109:1128	Interestingly, in silico percolation simulation for composite revealed interlinked 800 μm long-conduits predicting excellent absorption capacity and validated experimentally (640 % of composite dry weight).
33246777	3	18	theme	XPS	794:796	arg1	analysis					798:805	XPS analysis	794:805	XPS analysis	794:805	XPS analysis displayed the presence of SiNPs (Si2p peak for silicon) and calcium (Ca2p1, Ca2p3 transition peaks) in the composite.
33246777	10	19	theme	till	1804:1807	arg1	composite					1783:1791	The gamma irradiated composite	1762:1791	The gamma irradiated composite	1762:1791	The gamma irradiated composite was stable till 1.5 yr.
33246777	10	19	theme	till	1804:1807	arg1	1.5 yr					1809:1814	stable till 1.5 yr	1797:1814	stable till 1.5 yr	1797:1814	The gamma irradiated composite was stable till 1.5 yr.
33246777	2	20	theme	xerogel	688:694	arg1	composite					696:704	xerogel composite	688:704	xerogel composite	688:704	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	4	21	theme	composite	1109:1117	arg1	weight					1123:1128	composite dry weight	1109:1128	composite dry weight	1109:1128	Interestingly, in silico percolation simulation for composite revealed interlinked 800 μm long-conduits predicting excellent absorption capacity and validated experimentally (640 % of composite dry weight).
33246777	0	22	theme	blood	56:60	arg1	loss					62:65	blood loss	56:65	blood loss	56:65	Design and synthesis of a new topical agent for halting blood loss rapidly: A multimodal chitosan-gelatin xerogel composite loaded with silica nanoparticles and calcium.
33246777	9	23	theme	In	1539:1540	arg1	application					1547:1557	In vivo application	1539:1557	In vivo application of xerogel composite to lethal femoral artery injury in rats	1539:1618	In vivo application of xerogel composite to lethal femoral artery injury in rats achieved hemostasis (2.5 min) significantly faster than commercial Celox (3.3 min) and Gauze (4.6 min) and was easily removed from the wound.
33246777	3	24	theme	Si2p	840:843	arg1	peak					845:848	Si2p peak	840:848	Si2p peak	840:848	XPS analysis displayed the presence of SiNPs (Si2p peak for silicon) and calcium (Ca2p1, Ca2p3 transition peaks) in the composite.
33246777	2	25	theme	86.7	511:514	arg1	%					516:516	%	516:516	%	516:516	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	3	26	theme	SiNPs	833:837	arg1	presence					821:828	the presence	817:828	the presence of SiNPs (Si2p peak for silicon) and calcium (Ca2p1, Ca2p3 transition peaks) in the composite	817:922	XPS analysis displayed the presence of SiNPs (Si2p peak for silicon) and calcium (Ca2p1, Ca2p3 transition peaks) in the composite.
33246777	8	27	theme	high	1516:1519	arg1	biocompatibility					1521:1536	high biocompatibility	1516:1536	high biocompatibility	1516:1536	Moreover, xerogel composite showed high biocompatibility.
33246777	9	28	theme	xerogel	1562:1568	arg1	composite					1570:1578	xerogel composite	1562:1578	xerogel composite	1562:1578	In vivo application of xerogel composite to lethal femoral artery injury in rats achieved hemostasis (2.5 min) significantly faster than commercial Celox (3.3 min) and Gauze (4.6 min) and was easily removed from the wound.
33246777	2	29	theme	120 nm	638:643	arg1	SiNPs					631:635	SiNPs	631:635	SiNPs	631:635	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	2	29	theme	120 nm	638:643	arg1	size					645:648	120 nm size	638:648	120 nm size	638:648	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	11	30	theme	hemostatic	1899:1908	arg1	potential					1854:1862	potential	1854:1862	potential for application	1854:1878	Therefore, the xerogel composite has potential for application as a rapid topical hemostatic agent.
33246777	11	30	theme	hemostatic	1899:1908	arg1	agent					1910:1914	a rapid topical hemostatic agent	1883:1914	a rapid topical hemostatic agent	1883:1914	Therefore, the xerogel composite has potential for application as a rapid topical hemostatic agent.
33246777	11	31	theme	rapid	1885:1889	arg1	potential					1854:1862	potential	1854:1862	potential for application	1854:1878	Therefore, the xerogel composite has potential for application as a rapid topical hemostatic agent.
33246777	11	31	theme	rapid	1885:1889	arg1	agent					1910:1914	a rapid topical hemostatic agent	1883:1914	a rapid topical hemostatic agent	1883:1914	Therefore, the xerogel composite has potential for application as a rapid topical hemostatic agent.
33246777	2	32	theme	sodium	409:414	arg1	tripolyphosphate					416:431	sodium tripolyphosphate	409:431	sodium tripolyphosphate	409:431	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	11	33	contain	has	1850:1852	arg2	potential					1854:1862	potential	1854:1862	potential for application	1854:1878	Therefore, the xerogel composite has potential for application as a rapid topical hemostatic agent.
33246777	11	33	contain	has	1850:1852	arg1	composite					1840:1848	the xerogel composite	1828:1848	the xerogel composite	1828:1848	Therefore, the xerogel composite has potential for application as a rapid topical hemostatic agent.
33246777	11	33	contain	has	1850:1852	arg2	agent					1910:1914	a rapid topical hemostatic agent	1883:1914	a rapid topical hemostatic agent	1883:1914	Therefore, the xerogel composite has potential for application as a rapid topical hemostatic agent.
33246777	9	34	theme	femoral	1590:1596	arg1	injury					1605:1610	lethal femoral artery injury	1583:1610	lethal femoral artery injury in rats	1583:1618	In vivo application of xerogel composite to lethal femoral artery injury in rats achieved hemostasis (2.5 min) significantly faster than commercial Celox (3.3 min) and Gauze (4.6 min) and was easily removed from the wound.
33246777	2	35	theme	robust	456:461	arg1	xerogel					478:484	a robust, highly porous xerogel	454:484	a robust, highly porous xerogel	454:484	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	6	36	theme	generation	1365:1374	arg1	activities					1376:1385	good platelet activation and thrombin generation activities	1327:1385	good platelet activation and thrombin generation activities	1327:1385	The composite displayed good platelet activation and thrombin generation activities.
33246777	3	37	theme	calcium	867:873	arg1	presence					821:828	the presence	817:828	the presence of SiNPs (Si2p peak for silicon) and calcium (Ca2p1, Ca2p3 transition peaks) in the composite	817:922	XPS analysis displayed the presence of SiNPs (Si2p peak for silicon) and calcium (Ca2p1, Ca2p3 transition peaks) in the composite.
33246777	4	38	theme	interlinked	996:1006	arg1	800 μm long-conduits					1008:1027	interlinked 800 μm long-conduits	996:1027	interlinked 800 μm long-conduits predicting excellent absorption capacity and validated experimentally (640 % of composite dry weight)	996:1129	Interestingly, in silico percolation simulation for composite revealed interlinked 800 μm long-conduits predicting excellent absorption capacity and validated experimentally (640 % of composite dry weight).
33246777	1	39	theme	traumatic	220:228	arg1	injuries					230:237	traumatic injuries	220:237	traumatic injuries	220:237	Uncontrolled hemorrhage often causes death during traumatic injuries and halting exsanguination topically is a challenge.
33246777	2	40	with	chitosan	383:390	arg1	tripolyphosphate					416:431	sodium tripolyphosphate	409:431	sodium tripolyphosphate	409:431	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	0	41	dep	Design	0:5	arg1	composite					114:122	A multimodal chitosan-gelatin xerogel composite	76:122	Design and synthesis of a new topical agent for halting blood loss rapidly: A multimodal chitosan-gelatin xerogel composite loaded with silica nanoparticles and calcium.	0:168	Design and synthesis of a new topical agent for halting blood loss rapidly: A multimodal chitosan-gelatin xerogel composite loaded with silica nanoparticles and calcium.
33246777	3	42	theme	Ca2p3	883:887	arg1	Ca2p1					876:880	Ca2p1	876:880	Ca2p1	876:880	XPS analysis displayed the presence of SiNPs (Si2p peak for silicon) and calcium (Ca2p1, Ca2p3 transition peaks) in the composite.
33246777	3	42	theme	Ca2p3	883:887	arg1	peaks					900:904	Ca2p3 transition peaks	883:904	Ca2p3 transition peaks	883:904	XPS analysis displayed the presence of SiNPs (Si2p peak for silicon) and calcium (Ca2p1, Ca2p3 transition peaks) in the composite.
33246777	11	43	theme	xerogel	1832:1838	arg1	composite					1840:1848	the xerogel composite	1828:1848	the xerogel composite	1828:1848	Therefore, the xerogel composite has potential for application as a rapid topical hemostatic agent.
33246777	2	44	with	gelatin	396:402	arg1	tripolyphosphate					416:431	sodium tripolyphosphate	409:431	sodium tripolyphosphate	409:431	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	9	45	theme	commercial	1676:1685	arg1	3.3 min					1694:1700	3.3 min	1694:1700	3.3 min	1694:1700	In vivo application of xerogel composite to lethal femoral artery injury in rats achieved hemostasis (2.5 min) significantly faster than commercial Celox (3.3 min) and Gauze (4.6 min) and was easily removed from the wound.
33246777	9	45	theme	commercial	1676:1685	arg1	Celox					1687:1691	commercial Celox	1676:1691	commercial Celox (3.3 min)	1676:1701	In vivo application of xerogel composite to lethal femoral artery injury in rats achieved hemostasis (2.5 min) significantly faster than commercial Celox (3.3 min) and Gauze (4.6 min) and was easily removed from the wound.
33246777	5	46	theme	>16-fold	1155:1162	arg1	clotting					1179:1186	>16-fold improved blood clotting	1155:1186	>16-fold improved blood clotting	1155:1186	The composite achieved >16-fold improved blood clotting in vitro than commercial Celox and Gauze through multimodal interaction of its components with RBCs and platelets.
33246777	2	47	with	analysis	733:740	arg1	peaks					747:751	peaks	747:751	peaks	747:751	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	9	48	attach	removed	1738:1744	arg1	wound					1755:1759	the wound	1751:1759	the wound	1751:1759	In vivo application of xerogel composite to lethal femoral artery injury in rats achieved hemostasis (2.5 min) significantly faster than commercial Celox (3.3 min) and Gauze (4.6 min) and was easily removed from the wound.
33246777	9	48	attach	removed	1738:1744	arg2	application					1547:1557	In vivo application	1539:1557	In vivo application of xerogel composite to lethal femoral artery injury in rats	1539:1618	In vivo application of xerogel composite to lethal femoral artery injury in rats achieved hemostasis (2.5 min) significantly faster than commercial Celox (3.3 min) and Gauze (4.6 min) and was easily removed from the wound.
33246777	0	49	theme	topical	30:36	arg1	agent					38:42	a new topical agent	24:42	a new topical agent for halting blood loss rapidly	24:73	Design and synthesis of a new topical agent for halting blood loss rapidly: A multimodal chitosan-gelatin xerogel composite loaded with silica nanoparticles and calcium.
33246777	2	50	theme	synthesized	597:607	arg1	nanoparticles					616:628	0.5 mg synthesized silica nanoparticles	590:628	0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge)	590:664	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	7	51	theme	high	1401:1404	arg1	2.45 MPa					1428:1435	2.45 MPa	1428:1435	2.45 MPa	1428:1435	It displayed high compressive strength (2.45 MPa) and withstood pressure during application.
33246777	7	51	theme	high	1401:1404	arg1	strength					1418:1425	high compressive strength	1401:1425	high compressive strength (2.45 MPa)	1401:1436	It displayed high compressive strength (2.45 MPa) and withstood pressure during application.
33246777	6	52	theme	platelet	1332:1339	arg1	activation					1341:1350	platelet activation	1332:1350	platelet activation	1332:1350	The composite displayed good platelet activation and thrombin generation activities.
33246777	2	53	theme	topical	322:328	arg1	hemostat					330:337	an efficient multimodal topical hemostat	298:337	an efficient multimodal topical hemostat	298:337	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	1	54	theme	Uncontrolled	170:181	arg1	hemorrhage					183:192	Uncontrolled hemorrhage	170:192	Uncontrolled hemorrhage	170:192	Uncontrolled hemorrhage often causes death during traumatic injuries and halting exsanguination topically is a challenge.
33246777	2	55	dep	incorporating	576:588	arg1	iii					571:573	iii	571:573	iii	571:573	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	4	56	theme	absorption	1050:1059	arg1	capacity					1061:1068	excellent absorption capacity	1040:1068	excellent absorption capacity	1040:1068	Interestingly, in silico percolation simulation for composite revealed interlinked 800 μm long-conduits predicting excellent absorption capacity and validated experimentally (640 % of composite dry weight).
33246777	0	57	theme	xerogel	106:112	arg1	composite					114:122	A multimodal chitosan-gelatin xerogel composite	76:122	Design and synthesis of a new topical agent for halting blood loss rapidly: A multimodal chitosan-gelatin xerogel composite loaded with silica nanoparticles and calcium.	0:168	Design and synthesis of a new topical agent for halting blood loss rapidly: A multimodal chitosan-gelatin xerogel composite loaded with silica nanoparticles and calcium.
33246777	2	58	theme	efficient	301:309	arg1	hemostat					330:337	an efficient multimodal topical hemostat	298:337	an efficient multimodal topical hemostat	298:337	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	2	59	from	calcium	677:683	arg1	composite					696:704	xerogel composite	688:704	xerogel composite	688:704	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	2	60	theme	2.5 mM	670:675	arg1	calcium					677:683	2.5 mM calcium	670:683	2.5 mM calcium	670:683	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	0	61	theme	silica	136:141	arg1	nanoparticles					143:155	silica nanoparticles	136:155	silica nanoparticles	136:155	Design and synthesis of a new topical agent for halting blood loss rapidly: A multimodal chitosan-gelatin xerogel composite loaded with silica nanoparticles and calcium.
33246777	10	62	theme	gamma	1766:1770	arg1	composite					1783:1791	The gamma irradiated composite	1762:1791	The gamma irradiated composite	1762:1791	The gamma irradiated composite was stable till 1.5 yr.
33246777	10	62	theme	gamma	1766:1770	arg1	1.5 yr					1809:1814	stable till 1.5 yr	1797:1814	stable till 1.5 yr	1797:1814	The gamma irradiated composite was stable till 1.5 yr.
33246777	2	63	from	nanoparticles	616:628	arg1	composite					696:704	xerogel composite	688:704	xerogel composite	688:704	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	2	64	theme	large	544:548	arg1	pores					550:554	large pores	544:554	large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively	544:791	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	9	65	dep	In	1539:1540	arg1	vivo					1542:1545	vivo	1542:1545	vivo	1542:1545	In vivo application of xerogel composite to lethal femoral artery injury in rats achieved hemostasis (2.5 min) significantly faster than commercial Celox (3.3 min) and Gauze (4.6 min) and was easily removed from the wound.
33246777	5	66	theme	multimodal	1237:1246	arg1	interaction					1248:1258	multimodal interaction	1237:1258	multimodal interaction of its components with RBCs and platelets	1237:1300	The composite achieved >16-fold improved blood clotting in vitro than commercial Celox and Gauze through multimodal interaction of its components with RBCs and platelets.
33246777	6	67	theme	thrombin	1356:1363	arg1	generation					1365:1374	thrombin generation	1356:1374	thrombin generation	1356:1374	The composite displayed good platelet activation and thrombin generation activities.
33246777	10	68	theme	stable	1797:1802	arg1	composite					1783:1791	The gamma irradiated composite	1762:1791	The gamma irradiated composite	1762:1791	The gamma irradiated composite was stable till 1.5 yr.
33246777	10	68	theme	stable	1797:1802	arg1	1.5 yr					1809:1814	stable till 1.5 yr	1797:1814	stable till 1.5 yr	1797:1814	The gamma irradiated composite was stable till 1.5 yr.
33246777	9	69	theme	faster	1664:1669	arg1	2.5 min					1641:1647	2.5 min	1641:1647	2.5 min	1641:1647	In vivo application of xerogel composite to lethal femoral artery injury in rats achieved hemostasis (2.5 min) significantly faster than commercial Celox (3.3 min) and Gauze (4.6 min) and was easily removed from the wound.
33246777	9	69	theme	faster	1664:1669	arg1	hemostasis					1629:1638	hemostasis	1629:1638	hemostasis (2.5 min) significantly faster than commercial Celox (3.3 min) and Gauze (4.6 min)	1629:1721	In vivo application of xerogel composite to lethal femoral artery injury in rats achieved hemostasis (2.5 min) significantly faster than commercial Celox (3.3 min) and Gauze (4.6 min) and was easily removed from the wound.
33246777	4	70	theme	dry	1119:1121	arg1	weight					1123:1128	composite dry weight	1109:1128	composite dry weight	1109:1128	Interestingly, in silico percolation simulation for composite revealed interlinked 800 μm long-conduits predicting excellent absorption capacity and validated experimentally (640 % of composite dry weight).
33246777	5	71	theme	blood	1173:1177	arg1	clotting					1179:1186	>16-fold improved blood clotting	1155:1186	>16-fold improved blood clotting	1155:1186	The composite achieved >16-fold improved blood clotting in vitro than commercial Celox and Gauze through multimodal interaction of its components with RBCs and platelets.
33246777	6	72	theme	activation	1341:1350	arg1	activities					1376:1385	good platelet activation and thrombin generation activities	1327:1385	good platelet activation and thrombin generation activities	1327:1385	The composite displayed good platelet activation and thrombin generation activities.
33246777	2	73	dep	robust	456:461	arg1	porous					471:476	porous	471:476	porous	471:476	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	4	74	dep	validated	1074:1082	arg1	%					1104:1104	640 %	1100:1104	640 % of composite dry weight	1100:1128	Interestingly, in silico percolation simulation for composite revealed interlinked 800 μm long-conduits predicting excellent absorption capacity and validated experimentally (640 % of composite dry weight).
33246777	4	74	dep	validated	1074:1082	arg1	weight					1123:1128	composite dry weight	1109:1128	composite dry weight	1109:1128	Interestingly, in silico percolation simulation for composite revealed interlinked 800 μm long-conduits predicting excellent absorption capacity and validated experimentally (640 % of composite dry weight).
33246777	2	75	dep	nanoparticles	616:628	arg1	SiNPs					631:635	SiNPs	631:635	SiNPs	631:635	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	2	75	dep	nanoparticles	616:628	arg1	size					645:648	120 nm size	638:648	120 nm size	638:648	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	2	75	dep	nanoparticles	616:628	arg1	charge					658:663	-22 mV charge	651:663	-22 mV charge	651:663	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	8	76	theme	xerogel	1491:1497	arg1	composite					1499:1507	xerogel composite	1491:1507	xerogel composite	1491:1507	Moreover, xerogel composite showed high biocompatibility.
33246777	6	77	theme	good	1327:1330	arg1	activities					1376:1385	good platelet activation and thrombin generation activities	1327:1385	good platelet activation and thrombin generation activities	1327:1385	The composite displayed good platelet activation and thrombin generation activities.
33246777	2	78	theme	%	516:516	arg1	porosity					518:525	86.7 % porosity	511:525	86.7 % porosity	511:525	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	0	79	theme	multimodal	78:87	arg1	composite					114:122	A multimodal chitosan-gelatin xerogel composite	76:122	Design and synthesis of a new topical agent for halting blood loss rapidly: A multimodal chitosan-gelatin xerogel composite loaded with silica nanoparticles and calcium.	0:168	Design and synthesis of a new topical agent for halting blood loss rapidly: A multimodal chitosan-gelatin xerogel composite loaded with silica nanoparticles and calcium.
33246777	5	80	with	interaction	1248:1258	arg1	platelets					1292:1300	platelets	1292:1300	platelets	1292:1300	The composite achieved >16-fold improved blood clotting in vitro than commercial Celox and Gauze through multimodal interaction of its components with RBCs and platelets.
33246777	5	80	with	interaction	1248:1258	arg1	RBCs					1283:1286	RBCs	1283:1286	RBCs	1283:1286	The composite achieved >16-fold improved blood clotting in vitro than commercial Celox and Gauze through multimodal interaction of its components with RBCs and platelets.
33246777	2	81	theme	-22 mV	651:656	arg1	SiNPs					631:635	SiNPs	631:635	SiNPs	631:635	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	2	81	theme	-22 mV	651:656	arg1	charge					658:663	-22 mV charge	651:663	-22 mV charge	651:663	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	2	82	dep	fabricating	442:452	arg1	ii					438:439	ii	438:439	ii	438:439	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	4	83	dep	800 μm long-conduits	1008:1027	arg1	validated					1074:1082	validated	1074:1082	validated experimentally (640 % of composite dry weight)	1074:1129	Interestingly, in silico percolation simulation for composite revealed interlinked 800 μm long-conduits predicting excellent absorption capacity and validated experimentally (640 % of composite dry weight).
33246777	4	83	dep	800 μm long-conduits	1008:1027	arg1	predicting					1029:1038	predicting	1029:1038	predicting excellent absorption capacity	1029:1068	Interestingly, in silico percolation simulation for composite revealed interlinked 800 μm long-conduits predicting excellent absorption capacity and validated experimentally (640 % of composite dry weight).
33246777	9	84	theme	composite	1570:1578	arg1	application					1547:1557	In vivo application	1539:1557	In vivo application of xerogel composite to lethal femoral artery injury in rats	1539:1618	In vivo application of xerogel composite to lethal femoral artery injury in rats achieved hemostasis (2.5 min) significantly faster than commercial Celox (3.3 min) and Gauze (4.6 min) and was easily removed from the wound.
33246777	9	85	from	injury	1605:1610	arg1	rats					1615:1618	rats	1615:1618	rats	1615:1618	In vivo application of xerogel composite to lethal femoral artery injury in rats achieved hemostasis (2.5 min) significantly faster than commercial Celox (3.3 min) and Gauze (4.6 min) and was easily removed from the wound.
33246777	4	86	theme	in	940:941	arg1	simulation					962:971	in silico percolation simulation	940:971	in silico percolation simulation for composite	940:985	Interestingly, in silico percolation simulation for composite revealed interlinked 800 μm long-conduits predicting excellent absorption capacity and validated experimentally (640 % of composite dry weight).
33246777	2	87	from	cm-1	774:777	arg1	peaks					747:751	peaks	747:751	peaks	747:751	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	5	88	theme	components	1267:1276	arg1	interaction					1248:1258	multimodal interaction	1237:1258	multimodal interaction of its components with RBCs and platelets	1237:1300	The composite achieved >16-fold improved blood clotting in vitro than commercial Celox and Gauze through multimodal interaction of its components with RBCs and platelets.
33246777	0	89	theme	new	26:28	arg1	agent					38:42	a new topical agent	24:42	a new topical agent for halting blood loss rapidly	24:73	Design and synthesis of a new topical agent for halting blood loss rapidly: A multimodal chitosan-gelatin xerogel composite loaded with silica nanoparticles and calcium.
33246777	9	90	theme	lethal	1583:1588	arg1	injury					1605:1610	lethal femoral artery injury	1583:1610	lethal femoral artery injury in rats	1583:1618	In vivo application of xerogel composite to lethal femoral artery injury in rats achieved hemostasis (2.5 min) significantly faster than commercial Celox (3.3 min) and Gauze (4.6 min) and was easily removed from the wound.
33246777	11	91	theme	topical	1891:1897	arg1	potential					1854:1862	potential	1854:1862	potential for application	1854:1878	Therefore, the xerogel composite has potential for application as a rapid topical hemostatic agent.
33246777	11	91	theme	topical	1891:1897	arg1	agent					1910:1914	a rapid topical hemostatic agent	1883:1914	a rapid topical hemostatic agent	1883:1914	Therefore, the xerogel composite has potential for application as a rapid topical hemostatic agent.
33246777	0	92	theme	agent	38:42	arg1	synthesis					11:19	synthesis	11:19	synthesis	11:19	Design and synthesis of a new topical agent for halting blood loss rapidly: A multimodal chitosan-gelatin xerogel composite loaded with silica nanoparticles and calcium.
33246777	0	92	theme	agent	38:42	arg1	Design					0:5	Design	0:5	Design	0:5	Design and synthesis of a new topical agent for halting blood loss rapidly: A multimodal chitosan-gelatin xerogel composite loaded with silica nanoparticles and calcium.
33246777	9	93	theme	artery	1598:1603	arg1	injury					1605:1610	lethal femoral artery injury	1583:1610	lethal femoral artery injury in rats	1583:1618	In vivo application of xerogel composite to lethal femoral artery injury in rats achieved hemostasis (2.5 min) significantly faster than commercial Celox (3.3 min) and Gauze (4.6 min) and was easily removed from the wound.
33246777	10	94	theme	irradiated	1772:1781	arg1	composite					1783:1791	The gamma irradiated composite	1762:1791	The gamma irradiated composite	1762:1791	The gamma irradiated composite was stable till 1.5 yr.
33246777	10	94	theme	irradiated	1772:1781	arg1	1.5 yr					1809:1814	stable till 1.5 yr	1797:1814	stable till 1.5 yr	1797:1814	The gamma irradiated composite was stable till 1.5 yr.
33246777	2	95	dep	crosslinking	370:381	arg1	i					357:357	i	357:357	i	357:357	Here, an efficient multimodal topical hemostat was developed by (i) ionically crosslinking chitosan and gelatin with sodium tripolyphosphate for (ii) fabricating a robust, highly porous xerogel by lyophilization having 86.7 % porosity, by micro-CT and large pores ∼30 μm by SEM (iii) incorporating 0.5 mg synthesized silica nanoparticles (SiNPs, 120 nm size, -22 mV charge) and 2.5 mM calcium in xerogel composite that was confirmed by FTIR analysis with peaks at 3372, 986 and 788 cm-1, respectively.
33246777	1	96	theme	halting	243:249	arg1	challenge					281:289	a challenge	279:289	a challenge	279:289	Uncontrolled hemorrhage often causes death during traumatic injuries and halting exsanguination topically is a challenge.
33246777	1	96	theme	halting	243:249	arg1	exsanguination					251:264	halting exsanguination	243:264	halting exsanguination	243:264	Uncontrolled hemorrhage often causes death during traumatic injuries and halting exsanguination topically is a challenge.
33455380	0	0	theme	Hyaluronic	105:114	arg1	Hydrogel					130:137	Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel	81:137	Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel for Bone Regeneration	81:159	Integration of Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes with Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel for Bone Regeneration.
33455380	4	1	dep	in	767:768	arg1	situ					770:773	situ	770:773	situ	770:773	Considering the long period of bone regeneration, to effectively exert the reparative effect of exosomes, we synthesized an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites.
33455380	7	2	theme	new	1171:1173	arg1	strategy					1175:1182	a new strategy	1169:1182	a new strategy	1169:1182	Our experiment provides a new strategy for exosome-based therapy, which shows great potential in future tissue and organ repair.
33455380	7	3	from	potential	1229:1237	arg1	tissue					1249:1254	future tissue	1242:1254	future tissue	1242:1254	Our experiment provides a new strategy for exosome-based therapy, which shows great potential in future tissue and organ repair.
33455380	7	3	from	potential	1229:1237	arg1	repair					1266:1271	organ repair	1260:1271	organ repair	1260:1271	Our experiment provides a new strategy for exosome-based therapy, which shows great potential in future tissue and organ repair.
33455380	1	4	theme	defects	184:190	arg1	treatment					166:174	The treatment	162:174	The treatment of bone defects	162:190	The treatment of bone defects has plagued clinicians.
33455380	4	5	theme	bone	633:636	arg1	regeneration					638:649	bone regeneration	633:649	bone regeneration	633:649	Considering the long period of bone regeneration, to effectively exert the reparative effect of exosomes, we synthesized an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites.
33455380	0	6	theme	Hydroxyapatite-Embedded	81:103	arg1	Hydrogel					130:137	Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel	81:137	Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel for Bone Regeneration	81:159	Integration of Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes with Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel for Bone Regeneration.
33455380	5	7	theme	in	939:940	arg1	system					976:981	the HAP-embedded in situ cross-linked HA-ALG hydrogel system	922:981	the HAP-embedded in situ cross-linked HA-ALG hydrogel system	922:981	Then, we combined the exosomes with the HAP-embedded in situ cross-linked HA-ALG hydrogel system to repair bone defects in rats in vivo.
33455380	3	8	theme	calvariae	558:566	arg1	line					587:590	a murine calvariae preosteoblast cell line	549:590	a murine calvariae preosteoblast cell line	549:590	In this work, we successfully revealed that human umbilical cord mesenchymal stem cells-derived exosomes could effectively promote the proliferation, migration, and osteogenic differentiation of a murine calvariae preosteoblast cell line in vitro.
33455380	4	9	theme	injectable	726:735	arg1	system					831:836	an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system	723:836	an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites	723:883	Considering the long period of bone regeneration, to effectively exert the reparative effect of exosomes, we synthesized an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites.
33455380	5	10	theme	HAP-embedded	926:937	arg1	system					976:981	the HAP-embedded in situ cross-linked HA-ALG hydrogel system	922:981	the HAP-embedded in situ cross-linked HA-ALG hydrogel system	922:981	Then, we combined the exosomes with the HAP-embedded in situ cross-linked HA-ALG hydrogel system to repair bone defects in rats in vivo.
33455380	4	11	theme	acid-alginate	799:811	arg1	system					831:836	an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system	723:836	an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites	723:883	Considering the long period of bone regeneration, to effectively exert the reparative effect of exosomes, we synthesized an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites.
33455380	3	12	theme	stem	431:434	arg1	exosomes					450:457	human umbilical cord mesenchymal stem cells-derived exosomes	398:457	human umbilical cord mesenchymal stem cells-derived exosomes	398:457	In this work, we successfully revealed that human umbilical cord mesenchymal stem cells-derived exosomes could effectively promote the proliferation, migration, and osteogenic differentiation of a murine calvariae preosteoblast cell line in vitro.
33455380	0	13	theme	Acid-Alginate	116:128	arg1	Hydrogel					130:137	Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel	81:137	Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel for Bone Regeneration	81:159	Integration of Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes with Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel for Bone Regeneration.
33455380	4	14	theme	HA-ALG	814:819	arg1	system					831:836	an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system	723:836	an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites	723:883	Considering the long period of bone regeneration, to effectively exert the reparative effect of exosomes, we synthesized an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites.
33455380	3	15	theme	murine	551:556	arg1	line					587:590	a murine calvariae preosteoblast cell line	549:590	a murine calvariae preosteoblast cell line	549:590	In this work, we successfully revealed that human umbilical cord mesenchymal stem cells-derived exosomes could effectively promote the proliferation, migration, and osteogenic differentiation of a murine calvariae preosteoblast cell line in vitro.
33455380	4	16	theme	regeneration	638:649	arg1	period					623:628	the long period	614:628	the long period of bone regeneration	614:649	Considering the long period of bone regeneration, to effectively exert the reparative effect of exosomes, we synthesized an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites.
33455380	0	17	theme	Bone	143:146	arg1	Regeneration					148:159	Bone Regeneration	143:159	Bone Regeneration	143:159	Integration of Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes with Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel for Bone Regeneration.
33455380	5	18	theme	cross-linked	947:958	arg1	system					976:981	the HAP-embedded in situ cross-linked HA-ALG hydrogel system	922:981	the HAP-embedded in situ cross-linked HA-ALG hydrogel system	922:981	Then, we combined the exosomes with the HAP-embedded in situ cross-linked HA-ALG hydrogel system to repair bone defects in rats in vivo.
33455380	5	19	theme	repair	986:991	arg1	defects					998:1004	repair bone defects	986:1004	repair bone defects in rats	986:1012	Then, we combined the exosomes with the HAP-embedded in situ cross-linked HA-ALG hydrogel system to repair bone defects in rats in vivo.
33455380	6	20	theme	composite	1079:1087	arg1	hydrogel					1089:1096	composite hydrogel	1079:1096	composite hydrogel	1079:1096	The results showed that the combination of exosomes and composite hydrogel could significantly enhance bone regeneration.
33455380	2	21	theme	defect	304:309	arg1	regeneration					311:322	bone defect regeneration	299:322	bone defect regeneration	299:322	Exosomes, the naturally secreted nanovesicles by cells, exhibit great potential in bone defect regeneration to realize cell-free therapy.
33455380	6	22	theme	bone	1126:1129	arg1	regeneration					1131:1142	bone regeneration	1126:1142	bone regeneration	1126:1142	The results showed that the combination of exosomes and composite hydrogel could significantly enhance bone regeneration.
33455380	3	23	theme	cells-derived	436:448	arg1	exosomes					450:457	human umbilical cord mesenchymal stem cells-derived exosomes	398:457	human umbilical cord mesenchymal stem cells-derived exosomes	398:457	In this work, we successfully revealed that human umbilical cord mesenchymal stem cells-derived exosomes could effectively promote the proliferation, migration, and osteogenic differentiation of a murine calvariae preosteoblast cell line in vitro.
33455380	2	24	theme	bone	299:302	arg1	regeneration					311:322	bone defect regeneration	299:322	bone defect regeneration	299:322	Exosomes, the naturally secreted nanovesicles by cells, exhibit great potential in bone defect regeneration to realize cell-free therapy.
33455380	3	25	theme	cord	414:417	arg1	exosomes					450:457	human umbilical cord mesenchymal stem cells-derived exosomes	398:457	human umbilical cord mesenchymal stem cells-derived exosomes	398:457	In this work, we successfully revealed that human umbilical cord mesenchymal stem cells-derived exosomes could effectively promote the proliferation, migration, and osteogenic differentiation of a murine calvariae preosteoblast cell line in vitro.
33455380	3	26	theme	human	398:402	arg1	exosomes					450:457	human umbilical cord mesenchymal stem cells-derived exosomes	398:457	human umbilical cord mesenchymal stem cells-derived exosomes	398:457	In this work, we successfully revealed that human umbilical cord mesenchymal stem cells-derived exosomes could effectively promote the proliferation, migration, and osteogenic differentiation of a murine calvariae preosteoblast cell line in vitro.
33455380	4	27	theme	hyaluronic	788:797	arg1	system					831:836	an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system	723:836	an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites	723:883	Considering the long period of bone regeneration, to effectively exert the reparative effect of exosomes, we synthesized an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites.
33455380	7	28	theme	great	1223:1227	arg1	potential					1229:1237	great potential	1223:1237	great potential in future tissue and organ repair	1223:1271	Our experiment provides a new strategy for exosome-based therapy, which shows great potential in future tissue and organ repair.
33455380	3	29	theme	mesenchymal	419:429	arg1	exosomes					450:457	human umbilical cord mesenchymal stem cells-derived exosomes	398:457	human umbilical cord mesenchymal stem cells-derived exosomes	398:457	In this work, we successfully revealed that human umbilical cord mesenchymal stem cells-derived exosomes could effectively promote the proliferation, migration, and osteogenic differentiation of a murine calvariae preosteoblast cell line in vitro.
33455380	5	30	dep	in	939:940	arg1	situ					942:945	situ	942:945	situ	942:945	Then, we combined the exosomes with the HAP-embedded in situ cross-linked HA-ALG hydrogel system to repair bone defects in rats in vivo.
33455380	4	31	theme	cross-linked	775:786	arg1	system					831:836	an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system	723:836	an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites	723:883	Considering the long period of bone regeneration, to effectively exert the reparative effect of exosomes, we synthesized an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites.
33455380	0	32	theme	Umbilical	21:29	arg1	Exosomes					67:74	Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes	15:74	Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes	15:74	Integration of Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes with Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel for Bone Regeneration.
33455380	2	33	theme	cell-free	335:343	arg1	therapy					345:351	cell-free therapy	335:351	cell-free therapy	335:351	Exosomes, the naturally secreted nanovesicles by cells, exhibit great potential in bone defect regeneration to realize cell-free therapy.
33455380	6	34	theme	hydrogel	1089:1096	arg1	combination					1051:1061	the combination	1047:1061	the combination of exosomes and composite hydrogel	1047:1096	The results showed that the combination of exosomes and composite hydrogel could significantly enhance bone regeneration.
33455380	0	35	theme	Human	15:19	arg1	Exosomes					67:74	Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes	15:74	Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes	15:74	Integration of Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes with Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel for Bone Regeneration.
33455380	3	36	theme	line	587:590	arg1	migration					504:512	migration	504:512	migration	504:512	In this work, we successfully revealed that human umbilical cord mesenchymal stem cells-derived exosomes could effectively promote the proliferation, migration, and osteogenic differentiation of a murine calvariae preosteoblast cell line in vitro.
33455380	3	36	theme	line	587:590	arg1	proliferation					489:501	proliferation	489:501	proliferation	489:501	In this work, we successfully revealed that human umbilical cord mesenchymal stem cells-derived exosomes could effectively promote the proliferation, migration, and osteogenic differentiation of a murine calvariae preosteoblast cell line in vitro.
33455380	3	36	theme	line	587:590	arg1	differentiation					530:544	osteogenic differentiation	519:544	osteogenic differentiation	519:544	In this work, we successfully revealed that human umbilical cord mesenchymal stem cells-derived exosomes could effectively promote the proliferation, migration, and osteogenic differentiation of a murine calvariae preosteoblast cell line in vitro.
33455380	5	37	theme	bone	993:996	arg1	defects					998:1004	repair bone defects	986:1004	repair bone defects in rats	986:1012	Then, we combined the exosomes with the HAP-embedded in situ cross-linked HA-ALG hydrogel system to repair bone defects in rats in vivo.
33455380	4	38	link	cross-linked	775:786	arg1	system					831:836	an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system	723:836	an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites	723:883	Considering the long period of bone regeneration, to effectively exert the reparative effect of exosomes, we synthesized an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites.
33455380	4	39	theme	reparative	677:686	arg1	effect					688:693	the reparative effect	673:693	the reparative effect of exosomes	673:705	Considering the long period of bone regeneration, to effectively exert the reparative effect of exosomes, we synthesized an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites.
33455380	0	40	theme	Mesenchymal	36:46	arg1	Exosomes					67:74	Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes	15:74	Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes	15:74	Integration of Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes with Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel for Bone Regeneration.
33455380	4	41	theme	long	618:621	arg1	period					623:628	the long period	614:628	the long period of bone regeneration	614:649	Considering the long period of bone regeneration, to effectively exert the reparative effect of exosomes, we synthesized an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites.
33455380	2	42	from	potential	286:294	arg1	regeneration					311:322	bone defect regeneration	299:322	bone defect regeneration	299:322	Exosomes, the naturally secreted nanovesicles by cells, exhibit great potential in bone defect regeneration to realize cell-free therapy.
33455380	4	43	theme	exosomes	698:705	arg1	effect					688:693	the reparative effect	673:693	the reparative effect of exosomes	673:705	Considering the long period of bone regeneration, to effectively exert the reparative effect of exosomes, we synthesized an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites.
33455380	0	44	theme	Cord	31:34	arg1	Exosomes					67:74	Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes	15:74	Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes	15:74	Integration of Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes with Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel for Bone Regeneration.
33455380	3	45	link	cells-derived	436:448	arg1	exosomes					450:457	human umbilical cord mesenchymal stem cells-derived exosomes	398:457	human umbilical cord mesenchymal stem cells-derived exosomes	398:457	In this work, we successfully revealed that human umbilical cord mesenchymal stem cells-derived exosomes could effectively promote the proliferation, migration, and osteogenic differentiation of a murine calvariae preosteoblast cell line in vitro.
33455380	4	46	from	sites	879:883	arg1	exosomes					856:863	exosomes	856:863	exosomes at the defect sites	856:883	Considering the long period of bone regeneration, to effectively exert the reparative effect of exosomes, we synthesized an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites.
33455380	6	47	theme	exosomes	1066:1073	arg1	combination					1051:1061	the combination	1047:1061	the combination of exosomes and composite hydrogel	1047:1096	The results showed that the combination of exosomes and composite hydrogel could significantly enhance bone regeneration.
33455380	0	48	theme	Cells-Derived	53:65	arg1	Exosomes					67:74	Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes	15:74	Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes	15:74	Integration of Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes with Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel for Bone Regeneration.
33455380	5	49	with	exosomes	908:915	arg1	system					976:981	the HAP-embedded in situ cross-linked HA-ALG hydrogel system	922:981	the HAP-embedded in situ cross-linked HA-ALG hydrogel system	922:981	Then, we combined the exosomes with the HAP-embedded in situ cross-linked HA-ALG hydrogel system to repair bone defects in rats in vivo.
33455380	4	50	theme	-embedded	757:765	arg1	system					831:836	an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system	723:836	an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites	723:883	Considering the long period of bone regeneration, to effectively exert the reparative effect of exosomes, we synthesized an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites.
33455380	0	51	theme	Stem	48:51	arg1	Exosomes					67:74	Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes	15:74	Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes	15:74	Integration of Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes with Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel for Bone Regeneration.
33455380	7	52	theme	future	1242:1247	arg1	tissue					1249:1254	future tissue	1242:1254	future tissue	1242:1254	Our experiment provides a new strategy for exosome-based therapy, which shows great potential in future tissue and organ repair.
33455380	2	53	theme	secreted	240:247	arg1	nanovesicles					249:260	the naturally secreted nanovesicles	226:260	the naturally secreted nanovesicles by cells	226:269	Exosomes, the naturally secreted nanovesicles by cells, exhibit great potential in bone defect regeneration to realize cell-free therapy.
33455380	2	53	theme	secreted	240:247	arg1	Exosomes					216:223	Exosomes	216:223	Exosomes	216:223	Exosomes, the naturally secreted nanovesicles by cells, exhibit great potential in bone defect regeneration to realize cell-free therapy.
33455380	4	54	theme	defect	872:877	arg1	sites					879:883	the defect sites	868:883	the defect sites	868:883	Considering the long period of bone regeneration, to effectively exert the reparative effect of exosomes, we synthesized an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites.
33455380	5	55	theme	HA-ALG	960:965	arg1	system					976:981	the HAP-embedded in situ cross-linked HA-ALG hydrogel system	922:981	the HAP-embedded in situ cross-linked HA-ALG hydrogel system	922:981	Then, we combined the exosomes with the HAP-embedded in situ cross-linked HA-ALG hydrogel system to repair bone defects in rats in vivo.
33455380	5	56	link	cross-linked	947:958	arg1	system					976:981	the HAP-embedded in situ cross-linked HA-ALG hydrogel system	922:981	the HAP-embedded in situ cross-linked HA-ALG hydrogel system	922:981	Then, we combined the exosomes with the HAP-embedded in situ cross-linked HA-ALG hydrogel system to repair bone defects in rats in vivo.
33455380	7	57	theme	exosome-based	1188:1200	arg1	therapy					1202:1208	exosome-based therapy	1188:1208	exosome-based therapy	1188:1208	Our experiment provides a new strategy for exosome-based therapy, which shows great potential in future tissue and organ repair.
33455380	0	58	with	Integration	0:10	arg1	Hydrogel					130:137	Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel	81:137	Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel for Bone Regeneration	81:159	Integration of Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes with Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel for Bone Regeneration.
33455380	3	59	theme	osteogenic	519:528	arg1	differentiation					530:544	osteogenic differentiation	519:544	osteogenic differentiation	519:544	In this work, we successfully revealed that human umbilical cord mesenchymal stem cells-derived exosomes could effectively promote the proliferation, migration, and osteogenic differentiation of a murine calvariae preosteoblast cell line in vitro.
33455380	4	60	dep	-embedded	757:765	arg1	in					767:768	in	767:768	in	767:768	Considering the long period of bone regeneration, to effectively exert the reparative effect of exosomes, we synthesized an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites.
33455380	4	61	theme	hydrogel	822:829	arg1	system					831:836	an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system	723:836	an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites	723:883	Considering the long period of bone regeneration, to effectively exert the reparative effect of exosomes, we synthesized an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites.
33455380	7	62	theme	organ	1260:1264	arg1	repair					1266:1271	organ repair	1260:1271	organ repair	1260:1271	Our experiment provides a new strategy for exosome-based therapy, which shows great potential in future tissue and organ repair.
33455380	3	63	theme	cell	582:585	arg1	line					587:590	a murine calvariae preosteoblast cell line	549:590	a murine calvariae preosteoblast cell line	549:590	In this work, we successfully revealed that human umbilical cord mesenchymal stem cells-derived exosomes could effectively promote the proliferation, migration, and osteogenic differentiation of a murine calvariae preosteoblast cell line in vitro.
33455380	5	64	theme	hydrogel	967:974	arg1	system					976:981	the HAP-embedded in situ cross-linked HA-ALG hydrogel system	922:981	the HAP-embedded in situ cross-linked HA-ALG hydrogel system	922:981	Then, we combined the exosomes with the HAP-embedded in situ cross-linked HA-ALG hydrogel system to repair bone defects in rats in vivo.
33455380	2	65	theme	great	280:284	arg1	potential					286:294	great potential	280:294	great potential in bone defect regeneration to realize cell-free therapy	280:351	Exosomes, the naturally secreted nanovesicles by cells, exhibit great potential in bone defect regeneration to realize cell-free therapy.
33455380	3	66	theme	preosteoblast	568:580	arg1	line					587:590	a murine calvariae preosteoblast cell line	549:590	a murine calvariae preosteoblast cell line	549:590	In this work, we successfully revealed that human umbilical cord mesenchymal stem cells-derived exosomes could effectively promote the proliferation, migration, and osteogenic differentiation of a murine calvariae preosteoblast cell line in vitro.
33455380	5	67	from	defects	998:1004	arg1	rats					1009:1012	rats	1009:1012	rats	1009:1012	Then, we combined the exosomes with the HAP-embedded in situ cross-linked HA-ALG hydrogel system to repair bone defects in rats in vivo.
33455380	0	68	theme	Exosomes	67:74	arg1	Integration					0:10	Integration	0:10	Integration of Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes with Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel for Bone Regeneration.	0:160	Integration of Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes with Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel for Bone Regeneration.
33455380	3	69	theme	umbilical	404:412	arg1	exosomes					450:457	human umbilical cord mesenchymal stem cells-derived exosomes	398:457	human umbilical cord mesenchymal stem cells-derived exosomes	398:457	In this work, we successfully revealed that human umbilical cord mesenchymal stem cells-derived exosomes could effectively promote the proliferation, migration, and osteogenic differentiation of a murine calvariae preosteoblast cell line in vitro.
33455380	1	70	theme	bone	179:182	arg1	defects					184:190	bone defects	179:190	bone defects	179:190	The treatment of bone defects has plagued clinicians.
34973788	0	0	theme	thin	101:104	arg1	films					106:110	strong and tough cellulose nanofiber-based transparent thin films	46:110	strong and tough cellulose nanofiber-based transparent thin films with antifogging feature	46:135	Modulation of interfacial interactions toward strong and tough cellulose nanofiber-based transparent thin films with antifogging feature.
34973788	4	1	theme	10GA-8Gly-CNF	831:843	arg1	film					845:848	the 10GA-8Gly-CNF film	827:848	the 10GA-8Gly-CNF film	827:848	Among the prepared films, the 10GA-8Gly-CNF film exhibited the best results with regard to the enhancement in the tensile strength (21.1%), Young's modulus (10.6%), elongation at break (100%) and toughness (32.7%), as compared to the native CNF film.
34973788	3	2	theme	tough	495:499	arg1	films					518:522	strong and tough transparent thin films	484:522	strong and tough transparent thin films	484:522	However, in view of the growing demand for strong and tough transparent thin films, this article reported our study to develop films made from cellulose nanofiber (CNF) via tailoring the interfacial bonding interactions through the application of glycerol (Gly) and glutaraldehyde (GA), which functioned as a plasticizer and cross-linking agent, respectively.
34973788	5	3	theme	oxygen	1116:1121	arg1	film					1142:1145	radiofrequency oxygen plasma endowed the film	1101:1145	radiofrequency oxygen plasma endowed the film	1101:1145	Importantly, treating the surface of the film to radiofrequency oxygen plasma endowed the film with antifogging property, without compromising the optical clarity.
34973788	1	4	theme	functional	263:272	arg1	properties					274:283	their functional properties	257:283	their functional properties	257:283	Cross-linking is often performed to overcome the weak mechanical properties of native polymer films in order to expand their functional properties and applications.
34973788	0	5	theme	transparent	89:99	arg1	films					106:110	strong and tough cellulose nanofiber-based transparent thin films	46:110	strong and tough cellulose nanofiber-based transparent thin films with antifogging feature	46:135	Modulation of interfacial interactions toward strong and tough cellulose nanofiber-based transparent thin films with antifogging feature.
34973788	3	6	theme	transparent	501:511	arg1	films					518:522	strong and tough transparent thin films	484:522	strong and tough transparent thin films	484:522	However, in view of the growing demand for strong and tough transparent thin films, this article reported our study to develop films made from cellulose nanofiber (CNF) via tailoring the interfacial bonding interactions through the application of glycerol (Gly) and glutaraldehyde (GA), which functioned as a plasticizer and cross-linking agent, respectively.
34973788	5	7	theme	plasma	1123:1128	arg1	film					1142:1145	radiofrequency oxygen plasma endowed the film	1101:1145	radiofrequency oxygen plasma endowed the film	1101:1145	Importantly, treating the surface of the film to radiofrequency oxygen plasma endowed the film with antifogging property, without compromising the optical clarity.
34973788	5	8	theme	endowed	1130:1136	arg1	film					1142:1145	radiofrequency oxygen plasma endowed the film	1101:1145	radiofrequency oxygen plasma endowed the film	1101:1145	Importantly, treating the surface of the film to radiofrequency oxygen plasma endowed the film with antifogging property, without compromising the optical clarity.
34973788	3	9	theme	glutaraldehyde	707:720	arg1	application					673:683	the application	669:683	the application	669:683	However, in view of the growing demand for strong and tough transparent thin films, this article reported our study to develop films made from cellulose nanofiber (CNF) via tailoring the interfacial bonding interactions through the application of glycerol (Gly) and glutaraldehyde (GA), which functioned as a plasticizer and cross-linking agent, respectively.
34973788	3	9	theme	glutaraldehyde	707:720	arg1	agent					780:784	a plasticizer and cross-linking agent	748:784	agent	780:784	However, in view of the growing demand for strong and tough transparent thin films, this article reported our study to develop films made from cellulose nanofiber (CNF) via tailoring the interfacial bonding interactions through the application of glycerol (Gly) and glutaraldehyde (GA), which functioned as a plasticizer and cross-linking agent, respectively.
34973788	4	10	theme	prepared	811:818	arg1	films					820:824	the prepared films	807:824	the prepared films	807:824	Among the prepared films, the 10GA-8Gly-CNF film exhibited the best results with regard to the enhancement in the tensile strength (21.1%), Young's modulus (10.6%), elongation at break (100%) and toughness (32.7%), as compared to the native CNF film.
34973788	2	11	theme	high	423:426	arg1	brittleness					428:438	high brittleness	423:438	high brittleness	423:438	While this approach offers enhanced strength to the film, the film also suffers from low flexibility, low toughness and high brittleness.
34973788	5	12	theme	optical	1199:1205	arg1	clarity					1207:1213	the optical clarity	1195:1213	the optical clarity	1195:1213	Importantly, treating the surface of the film to radiofrequency oxygen plasma endowed the film with antifogging property, without compromising the optical clarity.
34973788	1	13	theme	weak	187:190	arg1	properties					203:212	the weak mechanical properties	183:212	the weak mechanical properties of native polymer films in order to expand their functional properties and applications	183:300	Cross-linking is often performed to overcome the weak mechanical properties of native polymer films in order to expand their functional properties and applications.
34973788	0	14	theme	antifogging	117:127	arg1	feature					129:135	antifogging feature	117:135	antifogging feature	117:135	Modulation of interfacial interactions toward strong and tough cellulose nanofiber-based transparent thin films with antifogging feature.
34973788	4	15	theme	Young	941:945	arg1	modulus					949:955	Young's modulus	941:955	Young's modulus (10.6%)	941:963	Among the prepared films, the 10GA-8Gly-CNF film exhibited the best results with regard to the enhancement in the tensile strength (21.1%), Young's modulus (10.6%), elongation at break (100%) and toughness (32.7%), as compared to the native CNF film.
34973788	4	15	theme	Young	941:945	arg1	%					962:962	10.6%	958:962	10.6%	958:962	Among the prepared films, the 10GA-8Gly-CNF film exhibited the best results with regard to the enhancement in the tensile strength (21.1%), Young's modulus (10.6%), elongation at break (100%) and toughness (32.7%), as compared to the native CNF film.
34973788	3	16	theme	strong	484:489	arg1	films					518:522	strong and tough transparent thin films	484:522	strong and tough transparent thin films	484:522	However, in view of the growing demand for strong and tough transparent thin films, this article reported our study to develop films made from cellulose nanofiber (CNF) via tailoring the interfacial bonding interactions through the application of glycerol (Gly) and glutaraldehyde (GA), which functioned as a plasticizer and cross-linking agent, respectively.
34973788	1	17	theme	mechanical	192:201	arg1	properties					203:212	the weak mechanical properties	183:212	the weak mechanical properties of native polymer films in order to expand their functional properties and applications	183:300	Cross-linking is often performed to overcome the weak mechanical properties of native polymer films in order to expand their functional properties and applications.
34973788	2	18	theme	low	388:390	arg1	flexibility					392:402	low flexibility	388:402	low flexibility	388:402	While this approach offers enhanced strength to the film, the film also suffers from low flexibility, low toughness and high brittleness.
34973788	0	19	theme	interactions	26:37	arg1	Modulation					0:9	Modulation	0:9	Modulation of interfacial interactions toward strong and tough cellulose nanofiber-based transparent thin films with antifogging feature.	0:136	Modulation of interfacial interactions toward strong and tough cellulose nanofiber-based transparent thin films with antifogging feature.
34973788	0	20	with	films	106:110	arg1	feature					129:135	antifogging feature	117:135	antifogging feature	117:135	Modulation of interfacial interactions toward strong and tough cellulose nanofiber-based transparent thin films with antifogging feature.
34973788	2	21	theme	enhanced	330:337	arg1	strength					339:346	enhanced strength	330:346	enhanced strength to the film	330:358	While this approach offers enhanced strength to the film, the film also suffers from low flexibility, low toughness and high brittleness.
34973788	1	22	theme	native	217:222	arg1	films					232:236	native polymer films	217:236	native polymer films in order to expand their functional properties and applications	217:300	Cross-linking is often performed to overcome the weak mechanical properties of native polymer films in order to expand their functional properties and applications.
34973788	0	23	theme	interfacial	14:24	arg1	interactions					26:37	interfacial interactions	14:37	interfacial interactions	14:37	Modulation of interfacial interactions toward strong and tough cellulose nanofiber-based transparent thin films with antifogging feature.
34973788	4	24	from	enhancement	896:906	arg1	strength					923:930	the tensile strength	911:930	the tensile strength (21.1%)	911:938	Among the prepared films, the 10GA-8Gly-CNF film exhibited the best results with regard to the enhancement in the tensile strength (21.1%), Young's modulus (10.6%), elongation at break (100%) and toughness (32.7%), as compared to the native CNF film.
34973788	4	24	from	enhancement	896:906	arg1	%					937:937	21.1%	933:937	21.1%	933:937	Among the prepared films, the 10GA-8Gly-CNF film exhibited the best results with regard to the enhancement in the tensile strength (21.1%), Young's modulus (10.6%), elongation at break (100%) and toughness (32.7%), as compared to the native CNF film.
34973788	1	25	theme	polymer	224:230	arg1	films					232:236	native polymer films	217:236	native polymer films in order to expand their functional properties and applications	217:300	Cross-linking is often performed to overcome the weak mechanical properties of native polymer films in order to expand their functional properties and applications.
34973788	0	26	theme	strong	46:51	arg1	films					106:110	strong and tough cellulose nanofiber-based transparent thin films	46:110	strong and tough cellulose nanofiber-based transparent thin films with antifogging feature	46:135	Modulation of interfacial interactions toward strong and tough cellulose nanofiber-based transparent thin films with antifogging feature.
34973788	3	27	theme	interfacial	628:638	arg1	interactions					648:659	the interfacial bonding interactions	624:659	the interfacial bonding interactions	624:659	However, in view of the growing demand for strong and tough transparent thin films, this article reported our study to develop films made from cellulose nanofiber (CNF) via tailoring the interfacial bonding interactions through the application of glycerol (Gly) and glutaraldehyde (GA), which functioned as a plasticizer and cross-linking agent, respectively.
34973788	1	28	theme	films	232:236	arg1	properties					203:212	the weak mechanical properties	183:212	the weak mechanical properties of native polymer films in order to expand their functional properties and applications	183:300	Cross-linking is often performed to overcome the weak mechanical properties of native polymer films in order to expand their functional properties and applications.
34973788	2	29	theme	low	405:407	arg1	toughness					409:417	low toughness	405:417	low toughness	405:417	While this approach offers enhanced strength to the film, the film also suffers from low flexibility, low toughness and high brittleness.
34973788	3	30	theme	cellulose	584:592	arg1	CNF					605:607	CNF	605:607	CNF	605:607	However, in view of the growing demand for strong and tough transparent thin films, this article reported our study to develop films made from cellulose nanofiber (CNF) via tailoring the interfacial bonding interactions through the application of glycerol (Gly) and glutaraldehyde (GA), which functioned as a plasticizer and cross-linking agent, respectively.
34973788	3	30	theme	cellulose	584:592	arg1	nanofiber					594:602	cellulose nanofiber	584:602	cellulose nanofiber (CNF)	584:608	However, in view of the growing demand for strong and tough transparent thin films, this article reported our study to develop films made from cellulose nanofiber (CNF) via tailoring the interfacial bonding interactions through the application of glycerol (Gly) and glutaraldehyde (GA), which functioned as a plasticizer and cross-linking agent, respectively.
34973788	4	31	theme	tensile	915:921	arg1	strength					923:930	the tensile strength	911:930	the tensile strength (21.1%)	911:938	Among the prepared films, the 10GA-8Gly-CNF film exhibited the best results with regard to the enhancement in the tensile strength (21.1%), Young's modulus (10.6%), elongation at break (100%) and toughness (32.7%), as compared to the native CNF film.
34973788	4	31	theme	tensile	915:921	arg1	%					937:937	21.1%	933:937	21.1%	933:937	Among the prepared films, the 10GA-8Gly-CNF film exhibited the best results with regard to the enhancement in the tensile strength (21.1%), Young's modulus (10.6%), elongation at break (100%) and toughness (32.7%), as compared to the native CNF film.
34973788	0	32	theme	tough	57:61	arg1	films					106:110	strong and tough cellulose nanofiber-based transparent thin films	46:110	strong and tough cellulose nanofiber-based transparent thin films with antifogging feature	46:135	Modulation of interfacial interactions toward strong and tough cellulose nanofiber-based transparent thin films with antifogging feature.
34973788	4	33	from	break	980:984	arg1	%					990:990	100%	987:990	100%	987:990	Among the prepared films, the 10GA-8Gly-CNF film exhibited the best results with regard to the enhancement in the tensile strength (21.1%), Young's modulus (10.6%), elongation at break (100%) and toughness (32.7%), as compared to the native CNF film.
34973788	4	33	from	break	980:984	arg1	elongation					966:975	elongation	966:975	elongation at break (100%)	966:991	Among the prepared films, the 10GA-8Gly-CNF film exhibited the best results with regard to the enhancement in the tensile strength (21.1%), Young's modulus (10.6%), elongation at break (100%) and toughness (32.7%), as compared to the native CNF film.
34973788	4	34	theme	best	864:867	arg1	results					869:875	the best results	860:875	the best results	860:875	Among the prepared films, the 10GA-8Gly-CNF film exhibited the best results with regard to the enhancement in the tensile strength (21.1%), Young's modulus (10.6%), elongation at break (100%) and toughness (32.7%), as compared to the native CNF film.
34973788	4	35	theme	CNF	1042:1044	arg1	film					1046:1049	the native CNF film	1031:1049	the native CNF film	1031:1049	Among the prepared films, the 10GA-8Gly-CNF film exhibited the best results with regard to the enhancement in the tensile strength (21.1%), Young's modulus (10.6%), elongation at break (100%) and toughness (32.7%), as compared to the native CNF film.
34973788	3	36	theme	growing	465:471	arg1	demand					473:478	the growing demand	461:478	the growing demand for strong and tough transparent thin films	461:522	However, in view of the growing demand for strong and tough transparent thin films, this article reported our study to develop films made from cellulose nanofiber (CNF) via tailoring the interfacial bonding interactions through the application of glycerol (Gly) and glutaraldehyde (GA), which functioned as a plasticizer and cross-linking agent, respectively.
34973788	3	37	theme	demand	473:478	arg1	view					453:456	view	453:456	view of the growing demand for strong and tough transparent thin films	453:522	However, in view of the growing demand for strong and tough transparent thin films, this article reported our study to develop films made from cellulose nanofiber (CNF) via tailoring the interfacial bonding interactions through the application of glycerol (Gly) and glutaraldehyde (GA), which functioned as a plasticizer and cross-linking agent, respectively.
34973788	3	38	theme	cross-linking	766:778	arg1	application					673:683	the application	669:683	the application	669:683	However, in view of the growing demand for strong and tough transparent thin films, this article reported our study to develop films made from cellulose nanofiber (CNF) via tailoring the interfacial bonding interactions through the application of glycerol (Gly) and glutaraldehyde (GA), which functioned as a plasticizer and cross-linking agent, respectively.
34973788	3	38	theme	cross-linking	766:778	arg1	agent					780:784	a plasticizer and cross-linking agent	748:784	agent	780:784	However, in view of the growing demand for strong and tough transparent thin films, this article reported our study to develop films made from cellulose nanofiber (CNF) via tailoring the interfacial bonding interactions through the application of glycerol (Gly) and glutaraldehyde (GA), which functioned as a plasticizer and cross-linking agent, respectively.
34973788	3	39	theme	bonding	640:646	arg1	interactions					648:659	the interfacial bonding interactions	624:659	the interfacial bonding interactions	624:659	However, in view of the growing demand for strong and tough transparent thin films, this article reported our study to develop films made from cellulose nanofiber (CNF) via tailoring the interfacial bonding interactions through the application of glycerol (Gly) and glutaraldehyde (GA), which functioned as a plasticizer and cross-linking agent, respectively.
34973788	0	40	theme	nanofiber-based	73:87	arg1	films					106:110	strong and tough cellulose nanofiber-based transparent thin films	46:110	strong and tough cellulose nanofiber-based transparent thin films with antifogging feature	46:135	Modulation of interfacial interactions toward strong and tough cellulose nanofiber-based transparent thin films with antifogging feature.
34973788	3	41	theme	thin	513:516	arg1	films					518:522	strong and tough transparent thin films	484:522	strong and tough transparent thin films	484:522	However, in view of the growing demand for strong and tough transparent thin films, this article reported our study to develop films made from cellulose nanofiber (CNF) via tailoring the interfacial bonding interactions through the application of glycerol (Gly) and glutaraldehyde (GA), which functioned as a plasticizer and cross-linking agent, respectively.
34973788	3	42	theme	plasticizer	750:760	arg1	application					673:683	the application	669:683	the application	669:683	However, in view of the growing demand for strong and tough transparent thin films, this article reported our study to develop films made from cellulose nanofiber (CNF) via tailoring the interfacial bonding interactions through the application of glycerol (Gly) and glutaraldehyde (GA), which functioned as a plasticizer and cross-linking agent, respectively.
34973788	3	42	theme	plasticizer	750:760	arg1	agent					780:784	a plasticizer and cross-linking agent	748:784	agent	780:784	However, in view of the growing demand for strong and tough transparent thin films, this article reported our study to develop films made from cellulose nanofiber (CNF) via tailoring the interfacial bonding interactions through the application of glycerol (Gly) and glutaraldehyde (GA), which functioned as a plasticizer and cross-linking agent, respectively.
34973788	0	43	theme	cellulose	63:71	arg1	films					106:110	strong and tough cellulose nanofiber-based transparent thin films	46:110	strong and tough cellulose nanofiber-based transparent thin films with antifogging feature	46:135	Modulation of interfacial interactions toward strong and tough cellulose nanofiber-based transparent thin films with antifogging feature.
34973788	3	44	theme	glycerol	688:695	arg1	application					673:683	the application	669:683	the application	669:683	However, in view of the growing demand for strong and tough transparent thin films, this article reported our study to develop films made from cellulose nanofiber (CNF) via tailoring the interfacial bonding interactions through the application of glycerol (Gly) and glutaraldehyde (GA), which functioned as a plasticizer and cross-linking agent, respectively.
34973788	3	44	theme	glycerol	688:695	arg1	agent					780:784	a plasticizer and cross-linking agent	748:784	agent	780:784	However, in view of the growing demand for strong and tough transparent thin films, this article reported our study to develop films made from cellulose nanofiber (CNF) via tailoring the interfacial bonding interactions through the application of glycerol (Gly) and glutaraldehyde (GA), which functioned as a plasticizer and cross-linking agent, respectively.
34973788	4	45	theme	native	1035:1040	arg1	film					1046:1049	the native CNF film	1031:1049	the native CNF film	1031:1049	Among the prepared films, the 10GA-8Gly-CNF film exhibited the best results with regard to the enhancement in the tensile strength (21.1%), Young's modulus (10.6%), elongation at break (100%) and toughness (32.7%), as compared to the native CNF film.
34973788	5	46	theme	film	1093:1096	arg1	surface					1078:1084	the surface	1074:1084	the surface of the film to radiofrequency oxygen plasma endowed the film	1074:1145	Importantly, treating the surface of the film to radiofrequency oxygen plasma endowed the film with antifogging property, without compromising the optical clarity.
34973788	5	47	theme	radiofrequency	1101:1114	arg1	film					1142:1145	radiofrequency oxygen plasma endowed the film	1101:1145	radiofrequency oxygen plasma endowed the film	1101:1145	Importantly, treating the surface of the film to radiofrequency oxygen plasma endowed the film with antifogging property, without compromising the optical clarity.
34492250	4	0	theme	chitosan	681:688	arg1	CMC					649:651	CMC	649:651	CMC	649:651	And the results indicated that relatively low DS of CMC and relatively low ratio of chitosan might be favorable for the adhesive properties of composite hydrogels.
34492250	4	0	theme	chitosan	681:688	arg1	ratio					672:676	relatively low ratio	657:676	relatively low ratio of chitosan	657:688	And the results indicated that relatively low DS of CMC and relatively low ratio of chitosan might be favorable for the adhesive properties of composite hydrogels.
34492250	2	1	theme	wrapping	366:373	arg1	applications					375:386	cigar wrapping applications	360:386	cigar wrapping applications	360:386	In this study, the potential of composite hydrogels prepared by carboxymethyl cellulose (CMC) and chitosan as glue for cigar wrapping applications was firstly studied.
34492250	0	2	theme	rheological	58:68	arg1	properties					88:97	the adhesive, rheological and antimicrobial properties	44:97	the adhesive, rheological and antimicrobial properties of carboxymethyl cellulose composite hydrogels	44:144	Exploring the role of chitosan in affecting the adhesive, rheological and antimicrobial properties of carboxymethyl cellulose composite hydrogels.
34492250	2	3	theme	cigar	360:364	arg1	applications					375:386	cigar wrapping applications	360:386	cigar wrapping applications	360:386	In this study, the potential of composite hydrogels prepared by carboxymethyl cellulose (CMC) and chitosan as glue for cigar wrapping applications was firstly studied.
34492250	8	4	theme	composite	1366:1374	arg1	hydrogel					1376:1383	CMC-chitosan composite hydrogel	1353:1383	CMC-chitosan composite hydrogel	1353:1383	All the information in this study is new, which could be useful for exploring the application of CMC-chitosan composite hydrogel in food, pharmaceutical, even other fields.
34492250	6	5	theme	chitosan	920:927	arg1	impacts					909:915	The impacts	905:915	The impacts of chitosan on the wet ability with tobacco leaf, the morphology and the XRD patterns of composite hydrogels	905:1024	The impacts of chitosan on the wet ability with tobacco leaf, the morphology and the XRD patterns of composite hydrogels were also observed.
34492250	3	6	theme	adhesive	503:510	arg1	performance					512:522	adhesive performance	503:522	adhesive performance	503:522	The impacts of degree of carboxymethyl substitution (DS) and the ratio of CMC:chitosan on the adhesive performance and rheological behaviors of composite hydrogels have been investigated.
34492250	3	7	dep	performance	512:522	arg1	the					499:501	the	499:501	the	499:501	The impacts of degree of carboxymethyl substitution (DS) and the ratio of CMC:chitosan on the adhesive performance and rheological behaviors of composite hydrogels have been investigated.
34492250	6	8	theme	XRD	990:992	arg1	patterns					994:1001	the XRD patterns	986:1001	the XRD patterns of composite hydrogels	986:1024	The impacts of chitosan on the wet ability with tobacco leaf, the morphology and the XRD patterns of composite hydrogels were also observed.
34492250	0	9	theme	antimicrobial	74:86	arg1	properties					88:97	the adhesive, rheological and antimicrobial properties	44:97	the adhesive, rheological and antimicrobial properties of carboxymethyl cellulose composite hydrogels	44:144	Exploring the role of chitosan in affecting the adhesive, rheological and antimicrobial properties of carboxymethyl cellulose composite hydrogels.
34492250	7	10	theme	tobacco	1130:1136	arg1	leaf					1138:1141	tobacco leaf	1130:1141	tobacco leaf brought by CMC	1130:1156	The CMC-chitosan composite hydrogel could significantly decrease the total molds on tobacco leaf brought by CMC, and therefore may show great potential to improve the quality of cigar during long-term storage.
34492250	2	11	theme	carboxymethyl	305:317	arg1	CMC					330:332	CMC	330:332	CMC	330:332	In this study, the potential of composite hydrogels prepared by carboxymethyl cellulose (CMC) and chitosan as glue for cigar wrapping applications was firstly studied.
34492250	2	11	theme	carboxymethyl	305:317	arg1	cellulose					319:327	carboxymethyl cellulose	305:327	carboxymethyl cellulose (CMC)	305:333	In this study, the potential of composite hydrogels prepared by carboxymethyl cellulose (CMC) and chitosan as glue for cigar wrapping applications was firstly studied.
34492250	0	12	theme	carboxymethyl	102:114	arg1	hydrogels					136:144	carboxymethyl cellulose composite hydrogels	102:144	carboxymethyl cellulose composite hydrogels	102:144	Exploring the role of chitosan in affecting the adhesive, rheological and antimicrobial properties of carboxymethyl cellulose composite hydrogels.
34492250	7	13	theme	total	1115:1119	arg1	molds					1121:1125	the total molds	1111:1125	the total molds on tobacco leaf brought by CMC	1111:1156	The CMC-chitosan composite hydrogel could significantly decrease the total molds on tobacco leaf brought by CMC, and therefore may show great potential to improve the quality of cigar during long-term storage.
34492250	4	14	theme	low	639:641	arg1	DS					643:644	relatively low DS	628:644	relatively low DS of CMC and relatively low ratio of chitosan	628:688	And the results indicated that relatively low DS of CMC and relatively low ratio of chitosan might be favorable for the adhesive properties of composite hydrogels.
34492250	3	15	theme	carboxymethyl	434:446	arg1	DS					462:463	DS	462:463	DS	462:463	The impacts of degree of carboxymethyl substitution (DS) and the ratio of CMC:chitosan on the adhesive performance and rheological behaviors of composite hydrogels have been investigated.
34492250	3	15	theme	carboxymethyl	434:446	arg1	substitution					448:459	carboxymethyl substitution	434:459	carboxymethyl substitution (DS)	434:464	The impacts of degree of carboxymethyl substitution (DS) and the ratio of CMC:chitosan on the adhesive performance and rheological behaviors of composite hydrogels have been investigated.
34492250	6	16	from	impacts	909:915	arg1	ability					940:946	the wet ability	932:946	the wet ability with tobacco leaf, the morphology and the XRD patterns of composite hydrogels	932:1024	The impacts of chitosan on the wet ability with tobacco leaf, the morphology and the XRD patterns of composite hydrogels were also observed.
34492250	3	17	theme	rheological	528:538	arg1	behaviors					540:548	rheological behaviors	528:548	rheological behaviors	528:548	The impacts of degree of carboxymethyl substitution (DS) and the ratio of CMC:chitosan on the adhesive performance and rheological behaviors of composite hydrogels have been investigated.
34492250	5	18	theme	higher	767:772	arg1	ratio					774:778	a higher ratio	765:778	a higher ratio of chitosan	765:790	But a higher ratio of chitosan may significantly improve the rheological properties of composite hydrogels and alter their thermal-sensitivity.
34492250	8	19	theme	CMC-chitosan	1353:1364	arg1	hydrogel					1376:1383	CMC-chitosan composite hydrogel	1353:1383	CMC-chitosan composite hydrogel	1353:1383	All the information in this study is new, which could be useful for exploring the application of CMC-chitosan composite hydrogel in food, pharmaceutical, even other fields.
34492250	7	20	theme	CMC-chitosan	1050:1061	arg1	hydrogel					1073:1080	The CMC-chitosan composite hydrogel	1046:1080	The CMC-chitosan composite hydrogel	1046:1080	The CMC-chitosan composite hydrogel could significantly decrease the total molds on tobacco leaf brought by CMC, and therefore may show great potential to improve the quality of cigar during long-term storage.
34492250	1	21	theme	food	203:206	arg1	applications					227:238	food and pharmaceutical applications	203:238	food and pharmaceutical applications	203:238	Natural polysaccharide-based hydrogels are promising in food and pharmaceutical applications.
34492250	7	22	theme	great	1182:1186	arg1	potential					1188:1196	great potential	1182:1196	great potential	1182:1196	The CMC-chitosan composite hydrogel could significantly decrease the total molds on tobacco leaf brought by CMC, and therefore may show great potential to improve the quality of cigar during long-term storage.
34492250	6	23	with	ability	940:946	arg1	patterns					994:1001	the XRD patterns	986:1001	the XRD patterns of composite hydrogels	986:1024	The impacts of chitosan on the wet ability with tobacco leaf, the morphology and the XRD patterns of composite hydrogels were also observed.
34492250	6	23	with	ability	940:946	arg1	leaf					961:964	tobacco leaf	953:964	tobacco leaf	953:964	The impacts of chitosan on the wet ability with tobacco leaf, the morphology and the XRD patterns of composite hydrogels were also observed.
34492250	6	23	with	ability	940:946	arg1	morphology					971:980	the morphology	967:980	the morphology	967:980	The impacts of chitosan on the wet ability with tobacco leaf, the morphology and the XRD patterns of composite hydrogels were also observed.
34492250	3	24	theme	substitution	448:459	arg1	degree					424:429	degree	424:429	degree of carboxymethyl substitution (DS)	424:464	The impacts of degree of carboxymethyl substitution (DS) and the ratio of CMC:chitosan on the adhesive performance and rheological behaviors of composite hydrogels have been investigated.
34492250	3	24	theme	substitution	448:459	arg1	ratio					474:478	the ratio	470:478	the ratio of CMC:chitosan	470:494	The impacts of degree of carboxymethyl substitution (DS) and the ratio of CMC:chitosan on the adhesive performance and rheological behaviors of composite hydrogels have been investigated.
34492250	0	25	theme	composite	126:134	arg1	hydrogels					136:144	carboxymethyl cellulose composite hydrogels	102:144	carboxymethyl cellulose composite hydrogels	102:144	Exploring the role of chitosan in affecting the adhesive, rheological and antimicrobial properties of carboxymethyl cellulose composite hydrogels.
34492250	3	26	theme	composite	553:561	arg1	hydrogels					563:571	composite hydrogels	553:571	composite hydrogels	553:571	The impacts of degree of carboxymethyl substitution (DS) and the ratio of CMC:chitosan on the adhesive performance and rheological behaviors of composite hydrogels have been investigated.
34492250	8	27	theme	food	1388:1391	arg1	fields					1421:1426	food, pharmaceutical, even other fields	1388:1426	food, pharmaceutical, even other fields	1388:1426	All the information in this study is new, which could be useful for exploring the application of CMC-chitosan composite hydrogel in food, pharmaceutical, even other fields.
34492250	1	28	theme	pharmaceutical	212:225	arg1	applications					227:238	food and pharmaceutical applications	203:238	food and pharmaceutical applications	203:238	Natural polysaccharide-based hydrogels are promising in food and pharmaceutical applications.
34492250	5	29	theme	rheological	822:832	arg1	properties					834:843	the rheological properties	818:843	the rheological properties of composite hydrogels	818:866	But a higher ratio of chitosan may significantly improve the rheological properties of composite hydrogels and alter their thermal-sensitivity.
34492250	0	30	theme	cellulose	116:124	arg1	hydrogels					136:144	carboxymethyl cellulose composite hydrogels	102:144	carboxymethyl cellulose composite hydrogels	102:144	Exploring the role of chitosan in affecting the adhesive, rheological and antimicrobial properties of carboxymethyl cellulose composite hydrogels.
34492250	3	31	theme	hydrogels	563:571	arg1	behaviors					540:548	rheological behaviors	528:548	rheological behaviors	528:548	The impacts of degree of carboxymethyl substitution (DS) and the ratio of CMC:chitosan on the adhesive performance and rheological behaviors of composite hydrogels have been investigated.
34492250	3	31	theme	hydrogels	563:571	arg1	performance					512:522	adhesive performance	503:522	adhesive performance	503:522	The impacts of degree of carboxymethyl substitution (DS) and the ratio of CMC:chitosan on the adhesive performance and rheological behaviors of composite hydrogels have been investigated.
34492250	3	32	theme	ratio	474:478	arg1	impacts					413:419	The impacts	409:419	The impacts of degree of carboxymethyl substitution (DS) and the ratio of CMC:chitosan on the adhesive performance and rheological behaviors of composite hydrogels	409:571	The impacts of degree of carboxymethyl substitution (DS) and the ratio of CMC:chitosan on the adhesive performance and rheological behaviors of composite hydrogels have been investigated.
34492250	3	33	theme	degree	424:429	arg1	impacts					413:419	The impacts	409:419	The impacts of degree of carboxymethyl substitution (DS) and the ratio of CMC:chitosan on the adhesive performance and rheological behaviors of composite hydrogels	409:571	The impacts of degree of carboxymethyl substitution (DS) and the ratio of CMC:chitosan on the adhesive performance and rheological behaviors of composite hydrogels have been investigated.
34492250	4	34	theme	ratio	672:676	arg1	DS					643:644	relatively low DS	628:644	relatively low DS of CMC and relatively low ratio of chitosan	628:688	And the results indicated that relatively low DS of CMC and relatively low ratio of chitosan might be favorable for the adhesive properties of composite hydrogels.
34492250	7	35	theme	composite	1063:1071	arg1	hydrogel					1073:1080	The CMC-chitosan composite hydrogel	1046:1080	The CMC-chitosan composite hydrogel	1046:1080	The CMC-chitosan composite hydrogel could significantly decrease the total molds on tobacco leaf brought by CMC, and therefore may show great potential to improve the quality of cigar during long-term storage.
34492250	5	36	theme	composite	848:856	arg1	hydrogels					858:866	composite hydrogels	848:866	composite hydrogels	848:866	But a higher ratio of chitosan may significantly improve the rheological properties of composite hydrogels and alter their thermal-sensitivity.
34492250	7	37	from	molds	1121:1125	arg1	leaf					1138:1141	tobacco leaf	1130:1141	tobacco leaf brought by CMC	1130:1156	The CMC-chitosan composite hydrogel could significantly decrease the total molds on tobacco leaf brought by CMC, and therefore may show great potential to improve the quality of cigar during long-term storage.
34492250	4	38	theme	low	668:670	arg1	ratio					672:676	relatively low ratio	657:676	relatively low ratio of chitosan	657:688	And the results indicated that relatively low DS of CMC and relatively low ratio of chitosan might be favorable for the adhesive properties of composite hydrogels.
34492250	4	39	theme	CMC	649:651	arg1	DS					643:644	relatively low DS	628:644	relatively low DS of CMC and relatively low ratio of chitosan	628:688	And the results indicated that relatively low DS of CMC and relatively low ratio of chitosan might be favorable for the adhesive properties of composite hydrogels.
34492250	0	40	theme	chitosan	22:29	arg1	role					14:17	the role	10:17	the role of chitosan in affecting the adhesive, rheological and antimicrobial properties of carboxymethyl cellulose composite hydrogels	10:144	Exploring the role of chitosan in affecting the adhesive, rheological and antimicrobial properties of carboxymethyl cellulose composite hydrogels.
34492250	0	41	theme	hydrogels	136:144	arg1	properties					88:97	the adhesive, rheological and antimicrobial properties	44:97	the adhesive, rheological and antimicrobial properties of carboxymethyl cellulose composite hydrogels	44:144	Exploring the role of chitosan in affecting the adhesive, rheological and antimicrobial properties of carboxymethyl cellulose composite hydrogels.
34492250	8	42	from	information	1264:1274	arg1	study					1284:1288	this study	1279:1288	this study	1279:1288	All the information in this study is new, which could be useful for exploring the application of CMC-chitosan composite hydrogel in food, pharmaceutical, even other fields.
34492250	5	43	theme	hydrogels	858:866	arg1	properties					834:843	the rheological properties	818:843	the rheological properties of composite hydrogels	818:866	But a higher ratio of chitosan may significantly improve the rheological properties of composite hydrogels and alter their thermal-sensitivity.
34492250	5	44	theme	chitosan	783:790	arg1	ratio					774:778	a higher ratio	765:778	a higher ratio of chitosan	765:790	But a higher ratio of chitosan may significantly improve the rheological properties of composite hydrogels and alter their thermal-sensitivity.
34492250	6	45	theme	tobacco	953:959	arg1	leaf					961:964	tobacco leaf	953:964	tobacco leaf	953:964	The impacts of chitosan on the wet ability with tobacco leaf, the morphology and the XRD patterns of composite hydrogels were also observed.
34492250	6	46	theme	wet	936:938	arg1	ability					940:946	the wet ability	932:946	the wet ability with tobacco leaf, the morphology and the XRD patterns of composite hydrogels	932:1024	The impacts of chitosan on the wet ability with tobacco leaf, the morphology and the XRD patterns of composite hydrogels were also observed.
34492250	3	47	theme	chitosan	487:494	arg1	degree					424:429	degree	424:429	degree of carboxymethyl substitution (DS)	424:464	The impacts of degree of carboxymethyl substitution (DS) and the ratio of CMC:chitosan on the adhesive performance and rheological behaviors of composite hydrogels have been investigated.
34492250	3	47	theme	chitosan	487:494	arg1	ratio					474:478	the ratio	470:478	the ratio of CMC:chitosan	470:494	The impacts of degree of carboxymethyl substitution (DS) and the ratio of CMC:chitosan on the adhesive performance and rheological behaviors of composite hydrogels have been investigated.
34492250	8	48	from	application	1338:1348	arg1	fields					1421:1426	food, pharmaceutical, even other fields	1388:1426	food, pharmaceutical, even other fields	1388:1426	All the information in this study is new, which could be useful for exploring the application of CMC-chitosan composite hydrogel in food, pharmaceutical, even other fields.
34492250	2	49	theme	hydrogels	283:291	arg1	potential					260:268	the potential	256:268	the potential of composite hydrogels prepared by carboxymethyl cellulose (CMC) and chitosan as glue for cigar wrapping applications	256:386	In this study, the potential of composite hydrogels prepared by carboxymethyl cellulose (CMC) and chitosan as glue for cigar wrapping applications was firstly studied.
34492250	1	50	from	applications	227:238	arg1	promising					190:198	promising	190:198	promising	190:198	Natural polysaccharide-based hydrogels are promising in food and pharmaceutical applications.
34492250	4	51	theme	adhesive	717:724	arg1	properties					726:735	the adhesive properties	713:735	the adhesive properties of composite hydrogels	713:758	And the results indicated that relatively low DS of CMC and relatively low ratio of chitosan might be favorable for the adhesive properties of composite hydrogels.
34492250	4	52	theme	hydrogels	750:758	arg1	properties					726:735	the adhesive properties	713:735	the adhesive properties of composite hydrogels	713:758	And the results indicated that relatively low DS of CMC and relatively low ratio of chitosan might be favorable for the adhesive properties of composite hydrogels.
34492250	8	53	dep	food	1388:1391	arg1	even					1410:1413	even	1410:1413	even	1410:1413	All the information in this study is new, which could be useful for exploring the application of CMC-chitosan composite hydrogel in food, pharmaceutical, even other fields.
34492250	8	53	dep	food	1388:1391	arg1	pharmaceutical					1394:1407	pharmaceutical	1394:1407	pharmaceutical	1394:1407	All the information in this study is new, which could be useful for exploring the application of CMC-chitosan composite hydrogel in food, pharmaceutical, even other fields.
34492250	2	54	theme	composite	273:281	arg1	hydrogels					283:291	composite hydrogels	273:291	composite hydrogels prepared by carboxymethyl cellulose (CMC) and chitosan	273:346	In this study, the potential of composite hydrogels prepared by carboxymethyl cellulose (CMC) and chitosan as glue for cigar wrapping applications was firstly studied.
34492250	4	55	theme	composite	740:748	arg1	hydrogels					750:758	composite hydrogels	740:758	composite hydrogels	740:758	And the results indicated that relatively low DS of CMC and relatively low ratio of chitosan might be favorable for the adhesive properties of composite hydrogels.
34492250	3	56	theme	CMC	483:485	arg1	chitosan					487:494	CMC:chitosan	483:494	CMC:chitosan	483:494	The impacts of degree of carboxymethyl substitution (DS) and the ratio of CMC:chitosan on the adhesive performance and rheological behaviors of composite hydrogels have been investigated.
34492250	7	57	theme	cigar	1224:1228	arg1	quality					1213:1219	the quality	1209:1219	the quality of cigar	1209:1228	The CMC-chitosan composite hydrogel could significantly decrease the total molds on tobacco leaf brought by CMC, and therefore may show great potential to improve the quality of cigar during long-term storage.
34492250	8	58	theme	hydrogel	1376:1383	arg1	application					1338:1348	the application	1334:1348	the application of CMC-chitosan composite hydrogel in food, pharmaceutical, even other fields	1334:1426	All the information in this study is new, which could be useful for exploring the application of CMC-chitosan composite hydrogel in food, pharmaceutical, even other fields.
34492250	1	59	theme	Natural	147:153	arg1	hydrogels					176:184	Natural polysaccharide-based hydrogels	147:184	Natural polysaccharide-based hydrogels	147:184	Natural polysaccharide-based hydrogels are promising in food and pharmaceutical applications.
34492250	6	60	theme	hydrogels	1016:1024	arg1	patterns					994:1001	the XRD patterns	986:1001	the XRD patterns of composite hydrogels	986:1024	The impacts of chitosan on the wet ability with tobacco leaf, the morphology and the XRD patterns of composite hydrogels were also observed.
34492250	6	60	theme	hydrogels	1016:1024	arg1	leaf					961:964	tobacco leaf	953:964	tobacco leaf	953:964	The impacts of chitosan on the wet ability with tobacco leaf, the morphology and the XRD patterns of composite hydrogels were also observed.
34492250	6	60	theme	hydrogels	1016:1024	arg1	morphology					971:980	the morphology	967:980	the morphology	967:980	The impacts of chitosan on the wet ability with tobacco leaf, the morphology and the XRD patterns of composite hydrogels were also observed.
34492250	0	61	theme	adhesive	48:55	arg1	properties					88:97	the adhesive, rheological and antimicrobial properties	44:97	the adhesive, rheological and antimicrobial properties of carboxymethyl cellulose composite hydrogels	44:144	Exploring the role of chitosan in affecting the adhesive, rheological and antimicrobial properties of carboxymethyl cellulose composite hydrogels.
34492250	3	62	from	impacts	413:419	arg1	behaviors					540:548	rheological behaviors	528:548	rheological behaviors	528:548	The impacts of degree of carboxymethyl substitution (DS) and the ratio of CMC:chitosan on the adhesive performance and rheological behaviors of composite hydrogels have been investigated.
34492250	3	62	from	impacts	413:419	arg1	performance					512:522	adhesive performance	503:522	adhesive performance	503:522	The impacts of degree of carboxymethyl substitution (DS) and the ratio of CMC:chitosan on the adhesive performance and rheological behaviors of composite hydrogels have been investigated.
34492250	8	63	theme	other	1415:1419	arg1	fields					1421:1426	food, pharmaceutical, even other fields	1388:1426	food, pharmaceutical, even other fields	1388:1426	All the information in this study is new, which could be useful for exploring the application of CMC-chitosan composite hydrogel in food, pharmaceutical, even other fields.
34492250	1	64	from	promising	190:198	arg1	applications					227:238	food and pharmaceutical applications	203:238	food and pharmaceutical applications	203:238	Natural polysaccharide-based hydrogels are promising in food and pharmaceutical applications.
34492250	1	65	theme	polysaccharide-based	155:174	arg1	hydrogels					176:184	Natural polysaccharide-based hydrogels	147:184	Natural polysaccharide-based hydrogels	147:184	Natural polysaccharide-based hydrogels are promising in food and pharmaceutical applications.
34492250	7	66	theme	long-term	1237:1245	arg1	storage					1247:1253	long-term storage	1237:1253	long-term storage	1237:1253	The CMC-chitosan composite hydrogel could significantly decrease the total molds on tobacco leaf brought by CMC, and therefore may show great potential to improve the quality of cigar during long-term storage.
34492250	6	67	theme	composite	1006:1014	arg1	hydrogels					1016:1024	composite hydrogels	1006:1024	composite hydrogels	1006:1024	The impacts of chitosan on the wet ability with tobacco leaf, the morphology and the XRD patterns of composite hydrogels were also observed.
32949364	0	0	theme	wastewater	108:117	arg1	treatment					119:127	wastewater treatment	108:127	wastewater treatment	108:127	Surfactant-facilitated alginate-biochar beads embedded with PAH-degrading bacteria and their application in wastewater treatment.
32949364	4	1	from	beads	719:723	arg1	absence					732:738	the absence	728:738	the absence of TX100	728:747	The removal ratio of acenaphthene was 24% higher for the TX100-facilitated immobilized bacterial beads than the beads in the absence of TX100.
32949364	7	2	theme	various	1132:1138	arg1	reactors					1161:1168	various wastewater treatment reactors	1132:1168	various wastewater treatment reactors	1132:1168	The new immobilized material can be widely used in various wastewater treatment reactors and in the in situ remediation of organic polluted water.
32949364	1	3	theme	composite	200:208	arg1	carriers					210:217	composite carriers	200:217	composite carriers	200:217	Immobilized Pseudomonas aeruginosa beads with alginate and biochar as composite carriers and a nonionic surfactant (TX100) as degradation promoter were prepared by the gel embedding method.
32949364	6	4	theme	application	1042:1052	arg1	results					1054:1060	better application results	1035:1060	better application results	1035:1060	The immobilized bacterial beads are suitable for continuous-flow reactors, and 2-mm-diameter beads will achieve better application results than larger beads.
32949364	5	5	from	PAHs	827:830	arg1	wastewater					835:844	wastewater	835:844	wastewater	835:844	The surfactant-facilitated immobilized bacterial beads can thoroughly remove PAHs in wastewater under the conditions of 10~50 °C, pH 2.5~10.5, and less than 0.2 mol/L NaCl.
32949364	1	6	theme	Pseudomonas	142:152	arg1	beads					165:169	Immobilized Pseudomonas aeruginosa beads	130:169	Immobilized Pseudomonas aeruginosa beads with alginate and biochar as composite carriers and a nonionic surfactant (TX100) as degradation promoter	130:275	Immobilized Pseudomonas aeruginosa beads with alginate and biochar as composite carriers and a nonionic surfactant (TX100) as degradation promoter were prepared by the gel embedding method.
32949364	6	7	theme	better	1035:1040	arg1	results					1054:1060	better application results	1035:1060	better application results	1035:1060	The immobilized bacterial beads are suitable for continuous-flow reactors, and 2-mm-diameter beads will achieve better application results than larger beads.
32949364	5	8	theme	immobilized	777:787	arg1	beads					799:803	The surfactant-facilitated immobilized bacterial beads	750:803	The surfactant-facilitated immobilized bacterial beads	750:803	The surfactant-facilitated immobilized bacterial beads can thoroughly remove PAHs in wastewater under the conditions of 10~50 °C, pH 2.5~10.5, and less than 0.2 mol/L NaCl.
32949364	6	9	theme	immobilized	927:937	arg1	beads					949:953	The immobilized bacterial beads	923:953	The immobilized bacterial beads	923:953	The immobilized bacterial beads are suitable for continuous-flow reactors, and 2-mm-diameter beads will achieve better application results than larger beads.
32949364	6	9	theme	immobilized	927:937	arg1	suitable					959:966	suitable	959:966	suitable	959:966	The immobilized bacterial beads are suitable for continuous-flow reactors, and 2-mm-diameter beads will achieve better application results than larger beads.
32949364	3	10	theme	PAH	573:575	arg1	degradation					577:587	PAH degradation	573:587	PAH degradation by P. aeruginosa	573:604	The addition of TX100 can simultaneously promote biochar sorption of PAHs and PAH degradation by P. aeruginosa.
32949364	1	11	theme	gel	298:300	arg1	method					312:317	the gel embedding method	294:317	the gel embedding method	294:317	Immobilized Pseudomonas aeruginosa beads with alginate and biochar as composite carriers and a nonionic surfactant (TX100) as degradation promoter were prepared by the gel embedding method.
32949364	5	12	theme	surfactant-facilitated	754:775	arg1	beads					799:803	The surfactant-facilitated immobilized bacterial beads	750:803	The surfactant-facilitated immobilized bacterial beads	750:803	The surfactant-facilitated immobilized bacterial beads can thoroughly remove PAHs in wastewater under the conditions of 10~50 °C, pH 2.5~10.5, and less than 0.2 mol/L NaCl.
32949364	0	13	from	bacteria	74:81	arg1	treatment					119:127	wastewater treatment	108:127	wastewater treatment	108:127	Surfactant-facilitated alginate-biochar beads embedded with PAH-degrading bacteria and their application in wastewater treatment.
32949364	7	14	theme	wastewater	1140:1149	arg1	reactors					1161:1168	various wastewater treatment reactors	1132:1168	various wastewater treatment reactors	1132:1168	The new immobilized material can be widely used in various wastewater treatment reactors and in the in situ remediation of organic polluted water.
32949364	4	15	theme	immobilized	682:692	arg1	beads					704:708	the TX100-facilitated immobilized bacterial beads	660:708	the TX100-facilitated immobilized bacterial beads	660:708	The removal ratio of acenaphthene was 24% higher for the TX100-facilitated immobilized bacterial beads than the beads in the absence of TX100.
32949364	4	16	theme	removal	611:617	arg1	ratio					619:623	The removal ratio	607:623	The removal ratio of acenaphthene	607:639	The removal ratio of acenaphthene was 24% higher for the TX100-facilitated immobilized bacterial beads than the beads in the absence of TX100.
32949364	4	16	theme	removal	611:617	arg1	higher					649:654	higher	649:654	higher	649:654	The removal ratio of acenaphthene was 24% higher for the TX100-facilitated immobilized bacterial beads than the beads in the absence of TX100.
32949364	7	17	theme	treatment	1151:1159	arg1	reactors					1161:1168	various wastewater treatment reactors	1132:1168	various wastewater treatment reactors	1132:1168	The new immobilized material can be widely used in various wastewater treatment reactors and in the in situ remediation of organic polluted water.
32949364	3	18	theme	PAHs	564:567	arg1	degradation					577:587	PAH degradation	573:587	PAH degradation by P. aeruginosa	573:604	The addition of TX100 can simultaneously promote biochar sorption of PAHs and PAH degradation by P. aeruginosa.
32949364	3	18	theme	PAHs	564:567	arg1	sorption					552:559	biochar sorption	544:559	biochar sorption of PAHs	544:567	The addition of TX100 can simultaneously promote biochar sorption of PAHs and PAH degradation by P. aeruginosa.
32949364	2	19	theme	suspension	427:436	arg1	suspension					427:436	the bacterial suspension	413:436	the bacterial suspension	413:436	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100 were 1%, OD600 = 1 and 200 mg/L, respectively.
32949364	2	19	theme	suspension	427:436	arg1	amount					380:385	the biochar addition amount	359:385	the biochar addition amount	359:385	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100 were 1%, OD600 = 1 and 200 mg/L, respectively.
32949364	2	19	theme	suspension	427:436	arg1	concentrations					395:408	the concentrations	391:408	the concentrations of the bacterial suspension and TX100	391:446	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100 were 1%, OD600 = 1 and 200 mg/L, respectively.
32949364	2	19	theme	suspension	427:436	arg1	TX100					442:446	TX100	442:446	TX100	442:446	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100 were 1%, OD600 = 1 and 200 mg/L, respectively.
32949364	0	20	theme	alginate-biochar	23:38	arg1	beads					40:44	Surfactant-facilitated alginate-biochar beads	0:44	Surfactant-facilitated alginate-biochar beads	0:44	Surfactant-facilitated alginate-biochar beads embedded with PAH-degrading bacteria and their application in wastewater treatment.
32949364	7	21	theme	water	1221:1225	arg1	remediation					1189:1199	the in situ remediation	1177:1199	the in situ remediation of organic polluted water	1177:1225	The new immobilized material can be widely used in various wastewater treatment reactors and in the in situ remediation of organic polluted water.
32949364	2	22	theme	bacterial	417:425	arg1	suspension					427:436	the bacterial suspension	413:436	the bacterial suspension	413:436	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100 were 1%, OD600 = 1 and 200 mg/L, respectively.
32949364	0	23	theme	Surfactant-facilitated	0:21	arg1	beads					40:44	Surfactant-facilitated alginate-biochar beads	0:44	Surfactant-facilitated alginate-biochar beads	0:44	Surfactant-facilitated alginate-biochar beads embedded with PAH-degrading bacteria and their application in wastewater treatment.
32949364	1	24	theme	embedding	302:310	arg1	method					312:317	the gel embedding method	294:317	the gel embedding method	294:317	Immobilized Pseudomonas aeruginosa beads with alginate and biochar as composite carriers and a nonionic surfactant (TX100) as degradation promoter were prepared by the gel embedding method.
32949364	1	25	with	beads	165:169	arg1	biochar					189:195	biochar	189:195	biochar	189:195	Immobilized Pseudomonas aeruginosa beads with alginate and biochar as composite carriers and a nonionic surfactant (TX100) as degradation promoter were prepared by the gel embedding method.
32949364	1	25	with	beads	165:169	arg1	alginate					176:183	alginate	176:183	alginate	176:183	Immobilized Pseudomonas aeruginosa beads with alginate and biochar as composite carriers and a nonionic surfactant (TX100) as degradation promoter were prepared by the gel embedding method.
32949364	5	26	theme	less	897:900	arg1	NaCl					917:920	less than 0.2 mol/L NaCl	897:920	less than 0.2 mol/L NaCl	897:920	The surfactant-facilitated immobilized bacterial beads can thoroughly remove PAHs in wastewater under the conditions of 10~50 °C, pH 2.5~10.5, and less than 0.2 mol/L NaCl.
32949364	1	27	theme	nonionic	225:232	arg1	TX100					246:250	TX100	246:250	TX100	246:250	Immobilized Pseudomonas aeruginosa beads with alginate and biochar as composite carriers and a nonionic surfactant (TX100) as degradation promoter were prepared by the gel embedding method.
32949364	1	27	theme	nonionic	225:232	arg1	surfactant					234:243	a nonionic surfactant	223:243	a nonionic surfactant (TX100)	223:251	Immobilized Pseudomonas aeruginosa beads with alginate and biochar as composite carriers and a nonionic surfactant (TX100) as degradation promoter were prepared by the gel embedding method.
32949364	7	28	used	used	1124:1127	arg2	material					1101:1108	The new immobilized material	1081:1108	The new immobilized material	1081:1108	The new immobilized material can be widely used in various wastewater treatment reactors and in the in situ remediation of organic polluted water.
32949364	2	29	theme	preparation	332:342	arg1	parameters					344:353	The optimal preparation parameters	320:353	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100	320:446	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100 were 1%, OD600 = 1 and 200 mg/L, respectively.
32949364	2	29	theme	preparation	332:342	arg1	%					454:454	1%	453:454	1%	453:454	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100 were 1%, OD600 = 1 and 200 mg/L, respectively.
32949364	5	30	theme	10~50 °C	870:877	arg1	conditions					856:865	the conditions	852:865	the conditions of 10~50 °C, pH 2.5~10.5, and less than 0.2 mol/L NaCl	852:920	The surfactant-facilitated immobilized bacterial beads can thoroughly remove PAHs in wastewater under the conditions of 10~50 °C, pH 2.5~10.5, and less than 0.2 mol/L NaCl.
32949364	7	31	theme	polluted	1212:1219	arg1	water					1221:1225	organic polluted water	1204:1225	organic polluted water	1204:1225	The new immobilized material can be widely used in various wastewater treatment reactors and in the in situ remediation of organic polluted water.
32949364	6	32	theme	2-mm-diameter	1002:1014	arg1	beads					1016:1020	2-mm-diameter beads	1002:1020	2-mm-diameter beads	1002:1020	The immobilized bacterial beads are suitable for continuous-flow reactors, and 2-mm-diameter beads will achieve better application results than larger beads.
32949364	2	33	theme	optimal	324:330	arg1	parameters					344:353	The optimal preparation parameters	320:353	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100	320:446	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100 were 1%, OD600 = 1 and 200 mg/L, respectively.
32949364	2	33	theme	optimal	324:330	arg1	%					454:454	1%	453:454	1%	453:454	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100 were 1%, OD600 = 1 and 200 mg/L, respectively.
32949364	5	34	theme	NaCl	917:920	arg1	conditions					856:865	the conditions	852:865	the conditions of 10~50 °C, pH 2.5~10.5, and less than 0.2 mol/L NaCl	852:920	The surfactant-facilitated immobilized bacterial beads can thoroughly remove PAHs in wastewater under the conditions of 10~50 °C, pH 2.5~10.5, and less than 0.2 mol/L NaCl.
32949364	0	35	theme	PAH-degrading	60:72	arg1	bacteria					74:81	PAH-degrading bacteria	60:81	PAH-degrading bacteria	60:81	Surfactant-facilitated alginate-biochar beads embedded with PAH-degrading bacteria and their application in wastewater treatment.
32949364	4	36	theme	bacterial	694:702	arg1	beads					704:708	the TX100-facilitated immobilized bacterial beads	660:708	the TX100-facilitated immobilized bacterial beads	660:708	The removal ratio of acenaphthene was 24% higher for the TX100-facilitated immobilized bacterial beads than the beads in the absence of TX100.
32949364	5	37	theme	pH 2.5~10.5	880:890	arg1	conditions					856:865	the conditions	852:865	the conditions of 10~50 °C, pH 2.5~10.5, and less than 0.2 mol/L NaCl	852:920	The surfactant-facilitated immobilized bacterial beads can thoroughly remove PAHs in wastewater under the conditions of 10~50 °C, pH 2.5~10.5, and less than 0.2 mol/L NaCl.
32949364	0	38	from	application	93:103	arg1	treatment					119:127	wastewater treatment	108:127	wastewater treatment	108:127	Surfactant-facilitated alginate-biochar beads embedded with PAH-degrading bacteria and their application in wastewater treatment.
32949364	7	39	theme	organic	1204:1210	arg1	water					1221:1225	organic polluted water	1204:1225	organic polluted water	1204:1225	The new immobilized material can be widely used in various wastewater treatment reactors and in the in situ remediation of organic polluted water.
32949364	0	40	with	embedded	46:53	arg1	bacteria					74:81	PAH-degrading bacteria	60:81	PAH-degrading bacteria	60:81	Surfactant-facilitated alginate-biochar beads embedded with PAH-degrading bacteria and their application in wastewater treatment.
32949364	0	40	with	embedded	46:53	arg1	application					93:103	their application	87:103	their application in wastewater treatment	87:127	Surfactant-facilitated alginate-biochar beads embedded with PAH-degrading bacteria and their application in wastewater treatment.
32949364	2	41	theme	TX100	442:446	arg1	suspension					427:436	the bacterial suspension	413:436	the bacterial suspension	413:436	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100 were 1%, OD600 = 1 and 200 mg/L, respectively.
32949364	2	41	theme	TX100	442:446	arg1	amount					380:385	the biochar addition amount	359:385	the biochar addition amount	359:385	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100 were 1%, OD600 = 1 and 200 mg/L, respectively.
32949364	2	41	theme	TX100	442:446	arg1	concentrations					395:408	the concentrations	391:408	the concentrations of the bacterial suspension and TX100	391:446	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100 were 1%, OD600 = 1 and 200 mg/L, respectively.
32949364	2	41	theme	TX100	442:446	arg1	TX100					442:446	TX100	442:446	TX100	442:446	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100 were 1%, OD600 = 1 and 200 mg/L, respectively.
32949364	7	42	theme	in	1181:1182	arg1	remediation					1189:1199	the in situ remediation	1177:1199	the in situ remediation of organic polluted water	1177:1225	The new immobilized material can be widely used in various wastewater treatment reactors and in the in situ remediation of organic polluted water.
32949364	7	43	theme	immobilized	1089:1099	arg1	material					1101:1108	The new immobilized material	1081:1108	The new immobilized material	1081:1108	The new immobilized material can be widely used in various wastewater treatment reactors and in the in situ remediation of organic polluted water.
32949364	3	44	theme	TX100	511:515	arg1	addition					499:506	The addition	495:506	The addition of TX100	495:515	The addition of TX100 can simultaneously promote biochar sorption of PAHs and PAH degradation by P. aeruginosa.
32949364	6	45	theme	bacterial	939:947	arg1	beads					949:953	The immobilized bacterial beads	923:953	The immobilized bacterial beads	923:953	The immobilized bacterial beads are suitable for continuous-flow reactors, and 2-mm-diameter beads will achieve better application results than larger beads.
32949364	6	45	theme	bacterial	939:947	arg1	suitable					959:966	suitable	959:966	suitable	959:966	The immobilized bacterial beads are suitable for continuous-flow reactors, and 2-mm-diameter beads will achieve better application results than larger beads.
32949364	6	46	theme	continuous-flow	972:986	arg1	reactors					988:995	continuous-flow reactors	972:995	continuous-flow reactors	972:995	The immobilized bacterial beads are suitable for continuous-flow reactors, and 2-mm-diameter beads will achieve better application results than larger beads.
32949364	2	47	theme	addition	371:378	arg1	suspension					427:436	the bacterial suspension	413:436	the bacterial suspension	413:436	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100 were 1%, OD600 = 1 and 200 mg/L, respectively.
32949364	2	47	theme	addition	371:378	arg1	amount					380:385	the biochar addition amount	359:385	the biochar addition amount	359:385	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100 were 1%, OD600 = 1 and 200 mg/L, respectively.
32949364	2	47	theme	addition	371:378	arg1	TX100					442:446	TX100	442:446	TX100	442:446	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100 were 1%, OD600 = 1 and 200 mg/L, respectively.
32949364	2	48	theme	biochar	363:369	arg1	suspension					427:436	the bacterial suspension	413:436	the bacterial suspension	413:436	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100 were 1%, OD600 = 1 and 200 mg/L, respectively.
32949364	2	48	theme	biochar	363:369	arg1	amount					380:385	the biochar addition amount	359:385	the biochar addition amount	359:385	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100 were 1%, OD600 = 1 and 200 mg/L, respectively.
32949364	2	48	theme	biochar	363:369	arg1	TX100					442:446	TX100	442:446	TX100	442:446	The optimal preparation parameters for the biochar addition amount and the concentrations of the bacterial suspension and TX100 were 1%, OD600 = 1 and 200 mg/L, respectively.
32949364	1	49	theme	Immobilized	130:140	arg1	beads					165:169	Immobilized Pseudomonas aeruginosa beads	130:169	Immobilized Pseudomonas aeruginosa beads with alginate and biochar as composite carriers and a nonionic surfactant (TX100) as degradation promoter	130:275	Immobilized Pseudomonas aeruginosa beads with alginate and biochar as composite carriers and a nonionic surfactant (TX100) as degradation promoter were prepared by the gel embedding method.
32949364	4	50	theme	acenaphthene	628:639	arg1	ratio					619:623	The removal ratio	607:623	The removal ratio of acenaphthene	607:639	The removal ratio of acenaphthene was 24% higher for the TX100-facilitated immobilized bacterial beads than the beads in the absence of TX100.
32949364	4	50	theme	acenaphthene	628:639	arg1	higher					649:654	higher	649:654	higher	649:654	The removal ratio of acenaphthene was 24% higher for the TX100-facilitated immobilized bacterial beads than the beads in the absence of TX100.
32949364	7	51	theme	new	1085:1087	arg1	material					1101:1108	The new immobilized material	1081:1108	The new immobilized material	1081:1108	The new immobilized material can be widely used in various wastewater treatment reactors and in the in situ remediation of organic polluted water.
32949364	4	52	theme	TX100	743:747	arg1	absence					732:738	the absence	728:738	the absence of TX100	728:747	The removal ratio of acenaphthene was 24% higher for the TX100-facilitated immobilized bacterial beads than the beads in the absence of TX100.
32949364	1	53	theme	degradation	256:266	arg1	promoter					268:275	degradation promoter	256:275	degradation promoter	256:275	Immobilized Pseudomonas aeruginosa beads with alginate and biochar as composite carriers and a nonionic surfactant (TX100) as degradation promoter were prepared by the gel embedding method.
32949364	5	54	theme	bacterial	789:797	arg1	beads					799:803	The surfactant-facilitated immobilized bacterial beads	750:803	The surfactant-facilitated immobilized bacterial beads	750:803	The surfactant-facilitated immobilized bacterial beads can thoroughly remove PAHs in wastewater under the conditions of 10~50 °C, pH 2.5~10.5, and less than 0.2 mol/L NaCl.
32949364	3	55	theme	biochar	544:550	arg1	sorption					552:559	biochar sorption	544:559	biochar sorption of PAHs	544:567	The addition of TX100 can simultaneously promote biochar sorption of PAHs and PAH degradation by P. aeruginosa.
32949364	4	56	theme	TX100-facilitated	664:680	arg1	beads					704:708	the TX100-facilitated immobilized bacterial beads	660:708	the TX100-facilitated immobilized bacterial beads	660:708	The removal ratio of acenaphthene was 24% higher for the TX100-facilitated immobilized bacterial beads than the beads in the absence of TX100.
32949364	6	57	theme	larger	1067:1072	arg1	beads					1074:1078	larger beads	1067:1078	larger beads	1067:1078	The immobilized bacterial beads are suitable for continuous-flow reactors, and 2-mm-diameter beads will achieve better application results than larger beads.
32949364	1	58	dep	Pseudomonas	142:152	arg1	aeruginosa					154:163	aeruginosa	154:163	aeruginosa	154:163	Immobilized Pseudomonas aeruginosa beads with alginate and biochar as composite carriers and a nonionic surfactant (TX100) as degradation promoter were prepared by the gel embedding method.
32949364	7	59	dep	in	1181:1182	arg1	situ					1184:1187	situ	1184:1187	situ	1184:1187	The new immobilized material can be widely used in various wastewater treatment reactors and in the in situ remediation of organic polluted water.
32278601	8	0	theme	%	1099:1099	arg1	crystallinity					1101:1113	74.2% crystallinity	1095:1113	74.2% crystallinity	1095:1113	Also, the rigidity had been improved from OL-BLF to OL-PUF, while with the highest for OL-MCC with 74.2% crystallinity, endowing it as a reliable load-bearing agent.
32278601	6	1	theme	fibril	884:889	arg1	structure					891:899	fibril structure	884:899	individually fibril structure	871:899	From physicochemical analysis, most lignin and hemicellulose components had been removed from OL-BLF to form OL-PUF with individually fibril structure.
32278601	10	2	theme	composite	1386:1394	arg1	applications					1396:1407	composite applications	1386:1407	composite applications	1386:1407	Therefore, olive MCC could act as a promising reinforcing agent to withstand harsh conditions for variety fields of composite applications.
32278601	8	3	theme	74.2	1095:1098	arg1	%					1099:1099	%	1099:1099	%	1099:1099	Also, the rigidity had been improved from OL-BLF to OL-PUF, while with the highest for OL-MCC with 74.2% crystallinity, endowing it as a reliable load-bearing agent.
32278601	1	4	contain	has	122:124	arg1	fiber					81:85	Olive fiber	75:85	Olive fiber	75:85	Olive fiber is a renewable natural fiber which has potential as an alternative biomass for extraction of microcrystalline cellulose (MCC).
32278601	1	4	contain	has	122:124	arg2	biomass					154:160	an alternative biomass	139:160	an alternative biomass for extraction of microcrystalline cellulose (MCC)	139:211	Olive fiber is a renewable natural fiber which has potential as an alternative biomass for extraction of microcrystalline cellulose (MCC).
32278601	1	4	contain	has	122:124	arg2	potential					126:134	potential	126:134	potential	126:134	Olive fiber is a renewable natural fiber which has potential as an alternative biomass for extraction of microcrystalline cellulose (MCC).
32278601	1	4	contain	has	122:124	arg1	fiber					110:114	a renewable natural fiber	90:114	a renewable natural fiber which has potential as an alternative biomass for extraction of microcrystalline cellulose (MCC)	90:211	Olive fiber is a renewable natural fiber which has potential as an alternative biomass for extraction of microcrystalline cellulose (MCC).
32278601	6	5	theme	most	781:784	arg1	components					811:820	most lignin and hemicellulose components	781:820	most lignin and hemicellulose components	781:820	From physicochemical analysis, most lignin and hemicellulose components had been removed from OL-BLF to form OL-PUF with individually fibril structure.
32278601	3	6	theme	present	345:351	arg1	study					353:357	present study	345:357	present study	345:357	At present study, a serial treatments of bleaching, alkaline and acid hydrolysis was employed to extract OL-BLF, OL-PUF, and OL-MCC respectively from olive stem fiber.
32278601	10	7	theme	promising	1306:1314	arg1	agent					1328:1332	a promising reinforcing agent	1304:1332	a promising reinforcing agent	1304:1332	Therefore, olive MCC could act as a promising reinforcing agent to withstand harsh conditions for variety fields of composite applications.
32278601	10	7	theme	promising	1306:1314	arg1	MCC					1287:1289	olive MCC	1281:1289	olive MCC	1281:1289	Therefore, olive MCC could act as a promising reinforcing agent to withstand harsh conditions for variety fields of composite applications.
32278601	7	8	theme	pure	946:949	arg1	component					961:969	highly pure cellulose component	939:969	highly pure cellulose component	939:969	The elemental analysis revealed that highly pure cellulose component was obtained for OL-MCC.
32278601	2	9	theme	small	277:281	arg1	size					295:298	its small dimensional size	273:298	its small dimensional size for ease of reactive fabrication process	273:339	MCC has been widely applied in various industries owing to its small dimensional size for ease of reactive fabrication process.
32278601	9	10	contain	had	1194:1196	arg1	OL-MCC					1187:1192	OL-MCC	1187:1192	OL-MCC	1187:1192	As for thermal analysis, OL-MCC had the most stable heat resistance in among the chemically-treated fibers.
32278601	9	10	contain	had	1194:1196	arg2	resistance					1219:1228	the most stable heat resistance	1198:1228	the most stable heat resistance in among the chemically-treated fibers	1198:1267	As for thermal analysis, OL-MCC had the most stable heat resistance in among the chemically-treated fibers.
32278601	4	11	theme	micro-crystallite	556:572	arg1	particles					574:582	short micro-crystallite particles	550:582	short micro-crystallite particles	550:582	In morphology examination, a feature of short micro-crystallite particles was obtained for OL-MCC.
32278601	10	12	theme	reinforcing	1316:1326	arg1	agent					1328:1332	a promising reinforcing agent	1304:1332	a promising reinforcing agent	1304:1332	Therefore, olive MCC could act as a promising reinforcing agent to withstand harsh conditions for variety fields of composite applications.
32278601	10	12	theme	reinforcing	1316:1326	arg1	MCC					1287:1289	olive MCC	1281:1289	olive MCC	1281:1289	Therefore, olive MCC could act as a promising reinforcing agent to withstand harsh conditions for variety fields of composite applications.
32278601	1	13	theme	Olive	75:79	arg1	fiber					81:85	Olive fiber	75:85	Olive fiber	75:85	Olive fiber is a renewable natural fiber which has potential as an alternative biomass for extraction of microcrystalline cellulose (MCC).
32278601	1	13	theme	Olive	75:79	arg1	fiber					110:114	a renewable natural fiber	90:114	a renewable natural fiber which has potential as an alternative biomass for extraction of microcrystalline cellulose (MCC)	90:211	Olive fiber is a renewable natural fiber which has potential as an alternative biomass for extraction of microcrystalline cellulose (MCC).
32278601	4	14	theme	short	550:554	arg1	particles					574:582	short micro-crystallite particles	550:582	short micro-crystallite particles	550:582	In morphology examination, a feature of short micro-crystallite particles was obtained for OL-MCC.
32278601	9	15	theme	chemically-treated	1243:1260	arg1	fibers					1262:1267	the chemically-treated fibers	1239:1267	the chemically-treated fibers	1239:1267	As for thermal analysis, OL-MCC had the most stable heat resistance in among the chemically-treated fibers.
32278601	6	16	theme	physicochemical	755:769	arg1	analysis					771:778	physicochemical analysis	755:778	physicochemical analysis	755:778	From physicochemical analysis, most lignin and hemicellulose components had been removed from OL-BLF to form OL-PUF with individually fibril structure.
32278601	3	17	theme	stem	498:501	arg1	fiber					503:507	olive stem fiber	492:507	olive stem fiber	492:507	At present study, a serial treatments of bleaching, alkaline and acid hydrolysis was employed to extract OL-BLF, OL-PUF, and OL-MCC respectively from olive stem fiber.
32278601	3	18	theme	hydrolysis	412:421	arg1	treatments					369:378	a serial treatments	360:378	a serial treatments of bleaching, alkaline and acid hydrolysis	360:421	At present study, a serial treatments of bleaching, alkaline and acid hydrolysis was employed to extract OL-BLF, OL-PUF, and OL-MCC respectively from olive stem fiber.
32278601	0	19	theme	olive	62:66	arg1	fiber					68:72	olive fiber	62:72	olive fiber	62:72	Characterization of microcrystalline cellulose extracted from olive fiber.
32278601	7	20	theme	elemental	906:914	arg1	analysis					916:923	The elemental analysis	902:923	The elemental analysis	902:923	The elemental analysis revealed that highly pure cellulose component was obtained for OL-MCC.
32278601	3	21	theme	serial	362:367	arg1	treatments					369:378	a serial treatments	360:378	a serial treatments of bleaching, alkaline and acid hydrolysis	360:421	At present study, a serial treatments of bleaching, alkaline and acid hydrolysis was employed to extract OL-BLF, OL-PUF, and OL-MCC respectively from olive stem fiber.
32278601	9	22	theme	stable	1207:1212	arg1	resistance					1219:1228	the most stable heat resistance	1198:1228	the most stable heat resistance in among the chemically-treated fibers	1198:1267	As for thermal analysis, OL-MCC had the most stable heat resistance in among the chemically-treated fibers.
32278601	1	23	theme	microcrystalline	180:195	arg1	MCC					208:210	MCC	208:210	MCC	208:210	Olive fiber is a renewable natural fiber which has potential as an alternative biomass for extraction of microcrystalline cellulose (MCC).
32278601	1	23	theme	microcrystalline	180:195	arg1	cellulose					197:205	microcrystalline cellulose	180:205	microcrystalline cellulose (MCC)	180:211	Olive fiber is a renewable natural fiber which has potential as an alternative biomass for extraction of microcrystalline cellulose (MCC).
32278601	5	24	theme	particle	613:620	arg1	size					622:625	The particle size	609:625	The particle size	609:625	The particle size was found gradually reducing from OL-PUF (305.31 μm) to OL-MCC (156.06 μm) due to the disintegration of cellulose fibrils.
32278601	10	25	theme	harsh	1347:1351	arg1	conditions					1353:1362	harsh conditions	1347:1362	harsh conditions for variety fields of composite applications	1347:1407	Therefore, olive MCC could act as a promising reinforcing agent to withstand harsh conditions for variety fields of composite applications.
32278601	1	26	theme	renewable	92:100	arg1	fiber					81:85	Olive fiber	75:85	Olive fiber	75:85	Olive fiber is a renewable natural fiber which has potential as an alternative biomass for extraction of microcrystalline cellulose (MCC).
32278601	1	26	theme	renewable	92:100	arg1	fiber					110:114	a renewable natural fiber	90:114	a renewable natural fiber which has potential as an alternative biomass for extraction of microcrystalline cellulose (MCC)	90:211	Olive fiber is a renewable natural fiber which has potential as an alternative biomass for extraction of microcrystalline cellulose (MCC).
32278601	3	27	theme	acid	407:410	arg1	hydrolysis					412:421	acid hydrolysis	407:421	acid hydrolysis	407:421	At present study, a serial treatments of bleaching, alkaline and acid hydrolysis was employed to extract OL-BLF, OL-PUF, and OL-MCC respectively from olive stem fiber.
32278601	0	28	theme	cellulose	37:45	arg1	Characterization					0:15	Characterization	0:15	Characterization of microcrystalline cellulose	0:45	Characterization of microcrystalline cellulose extracted from olive fiber.
32278601	4	29	theme	morphology	513:522	arg1	examination					524:534	morphology examination	513:534	morphology examination	513:534	In morphology examination, a feature of short micro-crystallite particles was obtained for OL-MCC.
32278601	8	30	with	OL-MCC	1083:1088	arg1	crystallinity					1101:1113	74.2% crystallinity	1095:1113	74.2% crystallinity	1095:1113	Also, the rigidity had been improved from OL-BLF to OL-PUF, while with the highest for OL-MCC with 74.2% crystallinity, endowing it as a reliable load-bearing agent.
32278601	1	31	theme	natural	102:108	arg1	fiber					81:85	Olive fiber	75:85	Olive fiber	75:85	Olive fiber is a renewable natural fiber which has potential as an alternative biomass for extraction of microcrystalline cellulose (MCC).
32278601	1	31	theme	natural	102:108	arg1	fiber					110:114	a renewable natural fiber	90:114	a renewable natural fiber which has potential as an alternative biomass for extraction of microcrystalline cellulose (MCC)	90:211	Olive fiber is a renewable natural fiber which has potential as an alternative biomass for extraction of microcrystalline cellulose (MCC).
32278601	10	32	theme	applications	1396:1407	arg1	variety					1368:1374	variety fields	1368:1381	variety fields of composite applications	1368:1407	Therefore, olive MCC could act as a promising reinforcing agent to withstand harsh conditions for variety fields of composite applications.
32278601	10	32	theme	applications	1396:1407	arg1	applications					1396:1407	composite applications	1386:1407	composite applications	1386:1407	Therefore, olive MCC could act as a promising reinforcing agent to withstand harsh conditions for variety fields of composite applications.
32278601	3	33	theme	extract	439:445	arg1	OL-BLF					447:452	extract OL-BLF	439:452	extract OL-BLF	439:452	At present study, a serial treatments of bleaching, alkaline and acid hydrolysis was employed to extract OL-BLF, OL-PUF, and OL-MCC respectively from olive stem fiber.
32278601	0	34	theme	microcrystalline	20:35	arg1	cellulose					37:45	microcrystalline cellulose	20:45	microcrystalline cellulose	20:45	Characterization of microcrystalline cellulose extracted from olive fiber.
32278601	9	35	theme	thermal	1169:1175	arg1	analysis					1177:1184	thermal analysis	1169:1184	thermal analysis	1169:1184	As for thermal analysis, OL-MCC had the most stable heat resistance in among the chemically-treated fibers.
32278601	2	36	theme	dimensional	283:293	arg1	size					295:298	its small dimensional size	273:298	its small dimensional size for ease of reactive fabrication process	273:339	MCC has been widely applied in various industries owing to its small dimensional size for ease of reactive fabrication process.
32278601	5	37	theme	cellulose	731:739	arg1	fibrils					741:747	cellulose fibrils	731:747	cellulose fibrils	731:747	The particle size was found gradually reducing from OL-PUF (305.31 μm) to OL-MCC (156.06 μm) due to the disintegration of cellulose fibrils.
32278601	10	38	dep	variety	1368:1374	arg1	fields					1376:1381	fields	1376:1381	fields	1376:1381	Therefore, olive MCC could act as a promising reinforcing agent to withstand harsh conditions for variety fields of composite applications.
32278601	1	39	theme	cellulose	197:205	arg1	extraction					166:175	extraction	166:175	extraction of microcrystalline cellulose (MCC)	166:211	Olive fiber is a renewable natural fiber which has potential as an alternative biomass for extraction of microcrystalline cellulose (MCC).
32278601	3	40	theme	alkaline	394:401	arg1	treatments					369:378	a serial treatments	360:378	a serial treatments of bleaching, alkaline and acid hydrolysis	360:421	At present study, a serial treatments of bleaching, alkaline and acid hydrolysis was employed to extract OL-BLF, OL-PUF, and OL-MCC respectively from olive stem fiber.
32278601	5	41	theme	fibrils	741:747	arg1	disintegration					713:726	the disintegration	709:726	the disintegration of cellulose fibrils	709:747	The particle size was found gradually reducing from OL-PUF (305.31 μm) to OL-MCC (156.06 μm) due to the disintegration of cellulose fibrils.
32278601	8	42	theme	load-bearing	1142:1153	arg1	agent					1155:1159	a reliable load-bearing agent	1131:1159	a reliable load-bearing agent	1131:1159	Also, the rigidity had been improved from OL-BLF to OL-PUF, while with the highest for OL-MCC with 74.2% crystallinity, endowing it as a reliable load-bearing agent.
32278601	8	42	theme	load-bearing	1142:1153	arg1	it					1125:1126	it	1125:1126	it	1125:1126	Also, the rigidity had been improved from OL-BLF to OL-PUF, while with the highest for OL-MCC with 74.2% crystallinity, endowing it as a reliable load-bearing agent.
32278601	10	43	theme	olive	1281:1285	arg1	agent					1328:1332	a promising reinforcing agent	1304:1332	a promising reinforcing agent	1304:1332	Therefore, olive MCC could act as a promising reinforcing agent to withstand harsh conditions for variety fields of composite applications.
32278601	10	43	theme	olive	1281:1285	arg1	MCC					1287:1289	olive MCC	1281:1289	olive MCC	1281:1289	Therefore, olive MCC could act as a promising reinforcing agent to withstand harsh conditions for variety fields of composite applications.
32278601	2	44	theme	process	333:339	arg1	ease					304:307	ease	304:307	ease of reactive fabrication process	304:339	MCC has been widely applied in various industries owing to its small dimensional size for ease of reactive fabrication process.
32278601	3	45	theme	bleaching	383:391	arg1	treatments					369:378	a serial treatments	360:378	a serial treatments of bleaching, alkaline and acid hydrolysis	360:421	At present study, a serial treatments of bleaching, alkaline and acid hydrolysis was employed to extract OL-BLF, OL-PUF, and OL-MCC respectively from olive stem fiber.
32278601	2	46	theme	fabrication	321:331	arg1	process					333:339	reactive fabrication process	312:339	reactive fabrication process	312:339	MCC has been widely applied in various industries owing to its small dimensional size for ease of reactive fabrication process.
32278601	6	47	theme	hemicellulose	797:809	arg1	components					811:820	most lignin and hemicellulose components	781:820	most lignin and hemicellulose components	781:820	From physicochemical analysis, most lignin and hemicellulose components had been removed from OL-BLF to form OL-PUF with individually fibril structure.
32278601	2	48	theme	reactive	312:319	arg1	process					333:339	reactive fabrication process	312:339	reactive fabrication process	312:339	MCC has been widely applied in various industries owing to its small dimensional size for ease of reactive fabrication process.
32278601	2	49	theme	various	245:251	arg1	industries					253:262	various industries	245:262	various industries owing to its small dimensional size for ease of reactive fabrication process	245:339	MCC has been widely applied in various industries owing to its small dimensional size for ease of reactive fabrication process.
32278601	3	50	theme	olive	492:496	arg1	fiber					503:507	olive stem fiber	492:507	olive stem fiber	492:507	At present study, a serial treatments of bleaching, alkaline and acid hydrolysis was employed to extract OL-BLF, OL-PUF, and OL-MCC respectively from olive stem fiber.
32278601	4	51	theme	particles	574:582	arg1	feature					539:545	a feature	537:545	a feature of short micro-crystallite particles	537:582	In morphology examination, a feature of short micro-crystallite particles was obtained for OL-MCC.
32278601	6	52	theme	lignin	786:791	arg1	components					811:820	most lignin and hemicellulose components	781:820	most lignin and hemicellulose components	781:820	From physicochemical analysis, most lignin and hemicellulose components had been removed from OL-BLF to form OL-PUF with individually fibril structure.
32278601	6	53	attach	removed	831:837	arg1	analysis					771:778	physicochemical analysis	755:778	physicochemical analysis	755:778	From physicochemical analysis, most lignin and hemicellulose components had been removed from OL-BLF to form OL-PUF with individually fibril structure.
32278601	6	53	attach	removed	831:837	arg1	OL-BLF					844:849	OL-BLF	844:849	OL-BLF	844:849	From physicochemical analysis, most lignin and hemicellulose components had been removed from OL-BLF to form OL-PUF with individually fibril structure.
32278601	6	53	attach	removed	831:837	arg2	components					811:820	most lignin and hemicellulose components	781:820	most lignin and hemicellulose components	781:820	From physicochemical analysis, most lignin and hemicellulose components had been removed from OL-BLF to form OL-PUF with individually fibril structure.
32278601	9	54	theme	heat	1214:1217	arg1	resistance					1219:1228	the most stable heat resistance	1198:1228	the most stable heat resistance in among the chemically-treated fibers	1198:1267	As for thermal analysis, OL-MCC had the most stable heat resistance in among the chemically-treated fibers.
32278601	8	55	theme	reliable	1133:1140	arg1	agent					1155:1159	a reliable load-bearing agent	1131:1159	a reliable load-bearing agent	1131:1159	Also, the rigidity had been improved from OL-BLF to OL-PUF, while with the highest for OL-MCC with 74.2% crystallinity, endowing it as a reliable load-bearing agent.
32278601	8	55	theme	reliable	1133:1140	arg1	it					1125:1126	it	1125:1126	it	1125:1126	Also, the rigidity had been improved from OL-BLF to OL-PUF, while with the highest for OL-MCC with 74.2% crystallinity, endowing it as a reliable load-bearing agent.
32278601	7	56	theme	cellulose	951:959	arg1	component					961:969	highly pure cellulose component	939:969	highly pure cellulose component	939:969	The elemental analysis revealed that highly pure cellulose component was obtained for OL-MCC.
32278601	1	57	theme	alternative	142:152	arg1	potential					126:134	potential	126:134	potential	126:134	Olive fiber is a renewable natural fiber which has potential as an alternative biomass for extraction of microcrystalline cellulose (MCC).
32278601	1	57	theme	alternative	142:152	arg1	biomass					154:160	an alternative biomass	139:160	an alternative biomass for extraction of microcrystalline cellulose (MCC)	139:211	Olive fiber is a renewable natural fiber which has potential as an alternative biomass for extraction of microcrystalline cellulose (MCC).
34354349	12	0	theme	NFs	1956:1958	arg1	mats					1960:1963	the PVA NFs mats	1948:1963	the PVA NFs mats containing 0.9% PE-CS-Au NPs	1948:1992	The human fibroblasts (HFF-1) showed the highest viability (123%) by seeding over the PVA NFs mats containing 0.9% PE-CS-Au NPs.
34354349	4	1	theme	further	862:868	arg1	studies					870:876	further studies	862:876	further studies	862:876	PE-CS-Au NPs were then dispersed into polyvinyl alcohol (PVA) solution at different ratios, where the optimized ratios were selected for electrospinning and further studies.
34354349	0	2	theme	Antibacterial	183:195	arg1	Properties					197:206	Antibacterial Properties	183:206	Antibacterial Properties	183:206	Chitosan/Gold Hybrid Nanoparticles Enriched Electrospun PVA Nanofibrous Mats for the Topical Delivery of Punica granatum L. Extract: Synthesis, Characterization, Biocompatibility and Antibacterial Properties.
34354349	10	3	theme	mathematical	1699:1710	arg1	model					1712:1716	the mathematical model	1695:1716	the mathematical model which could describe this release	1695:1750	The release of PE from NFs was controlled by a Fickian diffusion mechanism (n value ˂0.5), whereas Higuchi was the mathematical model which could describe this release.
34354349	10	3	theme	mathematical	1699:1710	arg1	Higuchi					1683:1689	Higuchi	1683:1689	Higuchi	1683:1689	The release of PE from NFs was controlled by a Fickian diffusion mechanism (n value ˂0.5), whereas Higuchi was the mathematical model which could describe this release.
34354349	1	4	theme	drug	276:279	arg1	system					290:295	Punica granatum L. extract (PE)-loaded drug delivery system	237:295	Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability	237:341	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	13	5	theme	suitable	2186:2193	arg1	composites					2064:2073	the electrospun PVA NFs composites	2040:2073	the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs	2040:2102	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	13	5	theme	suitable	2186:2193	arg1	scaffolds					2195:2203	suitable scaffolds	2186:2203	suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations	2186:2273	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	13	6	theme	NFs	2060:2062	arg1	scaffolds					2195:2203	suitable scaffolds	2186:2203	suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations	2186:2273	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	13	6	theme	NFs	2060:2062	arg1	composites					2064:2073	the electrospun PVA NFs composites	2040:2073	the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs	2040:2102	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	13	6	theme	NFs	2060:2062	arg1	agents					2133:2138	antibacterial agents	2119:2138	antibacterial agents against antibiotic-resistant bacteria	2119:2176	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	1	7	theme	biosynthesized	462:475	arg1	nanoparticles					488:500	biosynthesized PE-CS-gold nanoparticles	462:500	biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs)	462:515	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	1	7	theme	biosynthesized	462:475	arg1	NPs					512:514	PE-CS-Au NPs	503:514	PE-CS-Au NPs	503:514	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	7	8	theme	release	1163:1169	arg1	profiles					1171:1178	PE release profiles	1160:1178	PE release profiles	1160:1178	Swelling capacity, biodegradability, PE release profiles, release kinetics, antibacterial and cell biocompatibility were also demonstrated.
34354349	8	9	from	nm	1377:1378	arg1	PVA					1388:1390	neat PVA	1383:1390	neat PVA	1383:1390	RESULTS By incorporating PE-CS-Au NPs at 0.6% and 0.9%, the diameters of the nanofibers decreased from 295.7±83.1 nm in neat PVA to 165.6±43.4 and 147.8±42.7 nm, respectively.
34354349	13	10	theme	fibroblast	2252:2261	arg1	populations					2263:2273	fibroblast populations	2252:2273	fibroblast populations	2252:2273	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	1	11	theme	nanoparticles	488:500	arg1	amounts					451:457	different amounts	441:457	different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs)	441:515	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	1	11	theme	nanoparticles	488:500	arg1	nanoparticles					488:500	biosynthesized PE-CS-gold nanoparticles	462:500	biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs)	462:515	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	1	11	theme	nanoparticles	488:500	arg1	NPs					512:514	PE-CS-Au NPs	503:514	PE-CS-Au NPs	503:514	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	1	12	theme	PURPOSE	209:215	arg1	Intending					217:225	PURPOSE Intending	209:225	PURPOSE Intending	209:225	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	0	13	theme	Extract	124:130	arg1	Delivery					93:100	the Topical Delivery	81:100	the Topical Delivery of Punica granatum L. Extract	81:130	Chitosan/Gold Hybrid Nanoparticles Enriched Electrospun PVA Nanofibrous Mats for the Topical Delivery of Punica granatum L. Extract: Synthesis, Characterization, Biocompatibility and Antibacterial Properties.
34354349	1	14	theme	crosslinked	381:391	arg1	nanofibers					397:406	crosslinked PVA nanofibers	381:406	crosslinked PVA nanofibers (NFs)	381:412	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	1	14	theme	crosslinked	381:391	arg1	NFs					409:411	NFs	409:411	NFs	409:411	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	4	15	theme	PE-CS-Au	705:712	arg1	NPs					714:716	PE-CS-Au NPs	705:716	PE-CS-Au NPs	705:716	PE-CS-Au NPs were then dispersed into polyvinyl alcohol (PVA) solution at different ratios, where the optimized ratios were selected for electrospinning and further studies.
34354349	2	16	theme	PE-CS-Au	561:568	arg1	NPs					570:572	PE-CS-Au NPs	561:572	PE-CS-Au NPs	561:572	METHODS PE-conjugated CS-Au nanoparticles (PE-CS-Au NPs) were synthesized via green chemistry approach.
34354349	2	16	theme	PE-CS-Au	561:568	arg1	nanoparticles					546:558	PE-conjugated CS-Au nanoparticles	526:558	METHODS PE-conjugated CS-Au nanoparticles (PE-CS-Au NPs)	518:573	METHODS PE-conjugated CS-Au nanoparticles (PE-CS-Au NPs) were synthesized via green chemistry approach.
34354349	10	17	theme	PE	1599:1600	arg1	release					1588:1594	The release	1584:1594	The release of PE from NFs	1584:1609	The release of PE from NFs was controlled by a Fickian diffusion mechanism (n value ˂0.5), whereas Higuchi was the mathematical model which could describe this release.
34354349	1	18	theme	nanofibers	397:406	arg1	use					374:376	the use	370:376	the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs)	370:515	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	1	19	theme	Punica	237:242	arg1	system					290:295	Punica granatum L. extract (PE)-loaded drug delivery system	237:295	Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability	237:341	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	2	20	theme	green	596:600	arg1	approach					612:619	green chemistry approach	596:619	green chemistry approach	596:619	METHODS PE-conjugated CS-Au nanoparticles (PE-CS-Au NPs) were synthesized via green chemistry approach.
34354349	5	21	theme	PVA	915:917	arg1	NFs					931:933	NFs	931:933	NFs	931:933	Crosslinking of PE-CS-Au NPs loaded PVA nanofibers (NFs) was performed via glutaraldehyde vapor.
34354349	5	21	theme	PVA	915:917	arg1	nanofibers					919:928	PVA nanofibers	915:928	PVA nanofibers (NFs)	915:934	Crosslinking of PE-CS-Au NPs loaded PVA nanofibers (NFs) was performed via glutaraldehyde vapor.
34354349	3	22	theme	NPs	648:650	arg1	formation					626:634	The formation	622:634	The formation of PE-CS-Au NPs	622:650	The formation of PE-CS-Au NPs was confirmed by UV spectroscopy, DLS, SEM and STEM.
34354349	6	23	theme	thermal	1015:1021	arg1	stability					1023:1031	thermal stability	1015:1031	thermal stability	1015:1031	The morphology, chemical compositions, thermal stability and mechanical properties of PE-CS-Au NPs loaded NFs were evaluated by SEM, FTIR and DSC.
34354349	7	24	theme	cell	1217:1220	arg1	biocompatibility					1222:1237	cell biocompatibility	1217:1237	cell biocompatibility	1217:1237	Swelling capacity, biodegradability, PE release profiles, release kinetics, antibacterial and cell biocompatibility were also demonstrated.
34354349	12	25	theme	%	1979:1979	arg1	NPs					1990:1992	0.9% PE-CS-Au NPs	1976:1992	0.9% PE-CS-Au NPs	1976:1992	The human fibroblasts (HFF-1) showed the highest viability (123%) by seeding over the PVA NFs mats containing 0.9% PE-CS-Au NPs.
34354349	0	26	theme	Topical	85:91	arg1	Delivery					93:100	the Topical Delivery	81:100	the Topical Delivery of Punica granatum L. Extract	81:130	Chitosan/Gold Hybrid Nanoparticles Enriched Electrospun PVA Nanofibrous Mats for the Topical Delivery of Punica granatum L. Extract: Synthesis, Characterization, Biocompatibility and Antibacterial Properties.
34354349	1	27	theme	current	348:354	arg1	study					356:360	the current study	344:360	the current study	344:360	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	5	28	theme	NPs	904:906	arg1	Crosslinking					879:890	Crosslinking	879:890	Crosslinking of PE-CS-Au NPs loaded PVA nanofibers (NFs)	879:934	Crosslinking of PE-CS-Au NPs loaded PVA nanofibers (NFs) was performed via glutaraldehyde vapor.
34354349	13	29	theme	0.9	2086:2088	arg1	%					2089:2089	%	2089:2089	%	2089:2089	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	5	30	theme	glutaraldehyde	954:967	arg1	vapor					969:973	glutaraldehyde vapor	954:973	glutaraldehyde vapor	954:973	Crosslinking of PE-CS-Au NPs loaded PVA nanofibers (NFs) was performed via glutaraldehyde vapor.
34354349	2	31	theme	PE-conjugated	526:538	arg1	NPs					570:572	PE-CS-Au NPs	561:572	PE-CS-Au NPs	561:572	METHODS PE-conjugated CS-Au nanoparticles (PE-CS-Au NPs) were synthesized via green chemistry approach.
34354349	2	31	theme	PE-conjugated	526:538	arg1	nanoparticles					546:558	PE-conjugated CS-Au nanoparticles	526:558	METHODS PE-conjugated CS-Au nanoparticles (PE-CS-Au NPs)	518:573	METHODS PE-conjugated CS-Au nanoparticles (PE-CS-Au NPs) were synthesized via green chemistry approach.
34354349	11	32	theme	NPs	1861:1863	arg1	NPs					1861:1863	the incorporated PE-CS-Au NPs	1835:1863	the incorporated PE-CS-Au NPs	1835:1863	The antibacterial activity was found to be directly proportional to the amount of the incorporated PE-CS-Au NPs.
34354349	11	32	theme	NPs	1861:1863	arg1	amount					1825:1830	the amount	1821:1830	the amount of the incorporated PE-CS-Au NPs	1821:1863	The antibacterial activity was found to be directly proportional to the amount of the incorporated PE-CS-Au NPs.
34354349	0	33	theme	granatum	112:119	arg1	Extract					124:130	Punica granatum L. Extract	105:130	Punica granatum L. Extract	105:130	Chitosan/Gold Hybrid Nanoparticles Enriched Electrospun PVA Nanofibrous Mats for the Topical Delivery of Punica granatum L. Extract: Synthesis, Characterization, Biocompatibility and Antibacterial Properties.
34354349	6	34	theme	loaded	1075:1080	arg1	NFs					1082:1084	PE-CS-Au NPs loaded NFs	1062:1084	PE-CS-Au NPs loaded NFs	1062:1084	The morphology, chemical compositions, thermal stability and mechanical properties of PE-CS-Au NPs loaded NFs were evaluated by SEM, FTIR and DSC.
34354349	8	35	theme	nanofibers	1340:1349	arg1	diameters					1323:1331	the diameters	1319:1331	the diameters of the nanofibers	1319:1349	RESULTS By incorporating PE-CS-Au NPs at 0.6% and 0.9%, the diameters of the nanofibers decreased from 295.7±83.1 nm in neat PVA to 165.6±43.4 and 147.8±42.7 nm, respectively.
34354349	11	36	theme	incorporated	1839:1850	arg1	NPs					1861:1863	the incorporated PE-CS-Au NPs	1835:1863	the incorporated PE-CS-Au NPs	1835:1863	The antibacterial activity was found to be directly proportional to the amount of the incorporated PE-CS-Au NPs.
34354349	0	37	theme	Chitosan/Gold	0:12	arg1	Nanoparticles					21:33	Chitosan/Gold Hybrid Nanoparticles	0:33	Chitosan/Gold Hybrid Nanoparticles	0:33	Chitosan/Gold Hybrid Nanoparticles Enriched Electrospun PVA Nanofibrous Mats for the Topical Delivery of Punica granatum L. Extract: Synthesis, Characterization, Biocompatibility and Antibacterial Properties.
34354349	1	38	theme	delivery	281:288	arg1	system					290:295	Punica granatum L. extract (PE)-loaded drug delivery system	237:295	Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability	237:341	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	6	39	theme	PE-CS-Au	1062:1069	arg1	NPs					1071:1073	PE-CS-Au NPs	1062:1073	PE-CS-Au NPs loaded NFs	1062:1084	The morphology, chemical compositions, thermal stability and mechanical properties of PE-CS-Au NPs loaded NFs were evaluated by SEM, FTIR and DSC.
34354349	13	40	dep	CONCLUSION	1995:2004	arg1	suggest					2027:2033	suggest	2027:2033	suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations	2027:2273	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	4	41	theme	alcohol	753:759	arg1	solution					767:774	polyvinyl alcohol (PVA) solution	743:774	polyvinyl alcohol (PVA) solution at different ratios	743:794	PE-CS-Au NPs were then dispersed into polyvinyl alcohol (PVA) solution at different ratios, where the optimized ratios were selected for electrospinning and further studies.
34354349	0	42	dep	Mats	72:75	arg1	Synthesis					133:141	Synthesis	133:141	Synthesis	133:141	Chitosan/Gold Hybrid Nanoparticles Enriched Electrospun PVA Nanofibrous Mats for the Topical Delivery of Punica granatum L. Extract: Synthesis, Characterization, Biocompatibility and Antibacterial Properties.
34354349	0	42	dep	Mats	72:75	arg1	Biocompatibility					162:177	Biocompatibility	162:177	Biocompatibility	162:177	Chitosan/Gold Hybrid Nanoparticles Enriched Electrospun PVA Nanofibrous Mats for the Topical Delivery of Punica granatum L. Extract: Synthesis, Characterization, Biocompatibility and Antibacterial Properties.
34354349	0	42	dep	Mats	72:75	arg1	Properties					197:206	Antibacterial Properties	183:206	Antibacterial Properties	183:206	Chitosan/Gold Hybrid Nanoparticles Enriched Electrospun PVA Nanofibrous Mats for the Topical Delivery of Punica granatum L. Extract: Synthesis, Characterization, Biocompatibility and Antibacterial Properties.
34354349	0	42	dep	Mats	72:75	arg1	Characterization					144:159	Characterization	144:159	Characterization	144:159	Chitosan/Gold Hybrid Nanoparticles Enriched Electrospun PVA Nanofibrous Mats for the Topical Delivery of Punica granatum L. Extract: Synthesis, Characterization, Biocompatibility and Antibacterial Properties.
34354349	13	43	theme	antibiotic-resistant	2148:2167	arg1	bacteria					2169:2176	antibiotic-resistant bacteria	2148:2176	antibiotic-resistant bacteria	2148:2176	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	2	44	dep	METHODS	518:524	arg1	NPs					570:572	PE-CS-Au NPs	561:572	PE-CS-Au NPs	561:572	METHODS PE-conjugated CS-Au nanoparticles (PE-CS-Au NPs) were synthesized via green chemistry approach.
34354349	2	44	dep	METHODS	518:524	arg1	nanoparticles					546:558	PE-conjugated CS-Au nanoparticles	526:558	METHODS PE-conjugated CS-Au nanoparticles (PE-CS-Au NPs)	518:573	METHODS PE-conjugated CS-Au nanoparticles (PE-CS-Au NPs) were synthesized via green chemistry approach.
34354349	3	45	theme	UV	669:670	arg1	spectroscopy					672:683	UV spectroscopy	669:683	UV spectroscopy	669:683	The formation of PE-CS-Au NPs was confirmed by UV spectroscopy, DLS, SEM and STEM.
34354349	10	46	theme	Fickian	1631:1637	arg1	value					1662:1666	n value ˂0.5	1660:1671	n value ˂0.5	1660:1671	The release of PE from NFs was controlled by a Fickian diffusion mechanism (n value ˂0.5), whereas Higuchi was the mathematical model which could describe this release.
34354349	10	46	theme	Fickian	1631:1637	arg1	mechanism					1649:1657	a Fickian diffusion mechanism	1629:1657	a Fickian diffusion mechanism (n value ˂0.5)	1629:1672	The release of PE from NFs was controlled by a Fickian diffusion mechanism (n value ˂0.5), whereas Higuchi was the mathematical model which could describe this release.
34354349	1	47	theme	impact	307:312	arg1	system					290:295	Punica granatum L. extract (PE)-loaded drug delivery system	237:295	Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability	237:341	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	9	48	theme	NFs	1579:1581	arg1	stability					1527:1535	thermal stability	1519:1535	thermal stability	1519:1535	It is worth noting that crosslinking and incorporation of PE-CS-Au NPs improved thermal stability and mechanical properties of the obtained NFs.
34354349	9	48	theme	NFs	1579:1581	arg1	properties					1552:1561	mechanical properties	1541:1561	mechanical properties of the obtained NFs	1541:1581	It is worth noting that crosslinking and incorporation of PE-CS-Au NPs improved thermal stability and mechanical properties of the obtained NFs.
34354349	8	49	theme	neat	1383:1386	arg1	PVA					1388:1390	neat PVA	1383:1390	neat PVA	1383:1390	RESULTS By incorporating PE-CS-Au NPs at 0.6% and 0.9%, the diameters of the nanofibers decreased from 295.7±83.1 nm in neat PVA to 165.6±43.4 and 147.8±42.7 nm, respectively.
34354349	7	50	theme	Swelling	1123:1130	arg1	capacity					1132:1139	Swelling capacity	1123:1139	Swelling capacity	1123:1139	Swelling capacity, biodegradability, PE release profiles, release kinetics, antibacterial and cell biocompatibility were also demonstrated.
34354349	1	51	theme	biomedical	318:327	arg1	applicability					329:341	biomedical applicability	318:341	biomedical applicability	318:341	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	4	52	theme	different	779:787	arg1	ratios					789:794	different ratios	779:794	different ratios	779:794	PE-CS-Au NPs were then dispersed into polyvinyl alcohol (PVA) solution at different ratios, where the optimized ratios were selected for electrospinning and further studies.
34354349	10	53	theme	n	1660:1660	arg1	value					1662:1666	n value ˂0.5	1660:1671	n value ˂0.5	1660:1671	The release of PE from NFs was controlled by a Fickian diffusion mechanism (n value ˂0.5), whereas Higuchi was the mathematical model which could describe this release.
34354349	10	53	theme	n	1660:1660	arg1	mechanism					1649:1657	a Fickian diffusion mechanism	1629:1657	a Fickian diffusion mechanism (n value ˂0.5)	1629:1672	The release of PE from NFs was controlled by a Fickian diffusion mechanism (n value ˂0.5), whereas Higuchi was the mathematical model which could describe this release.
34354349	1	54	theme	granatum	244:251	arg1	system					290:295	Punica granatum L. extract (PE)-loaded drug delivery system	237:295	Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability	237:341	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	1	55	theme	extract	256:262	arg1	system					290:295	Punica granatum L. extract (PE)-loaded drug delivery system	237:295	Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability	237:341	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	1	56	theme	PE	265:266	arg1	system					290:295	Punica granatum L. extract (PE)-loaded drug delivery system	237:295	Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability	237:341	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	12	57	theme	PVA	1952:1954	arg1	mats					1960:1963	the PVA NFs mats	1948:1963	the PVA NFs mats containing 0.9% PE-CS-Au NPs	1948:1992	The human fibroblasts (HFF-1) showed the highest viability (123%) by seeding over the PVA NFs mats containing 0.9% PE-CS-Au NPs.
34354349	1	58	theme	different	441:449	arg1	amounts					451:457	different amounts	441:457	different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs)	441:515	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	1	58	theme	different	441:449	arg1	nanoparticles					488:500	biosynthesized PE-CS-gold nanoparticles	462:500	biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs)	462:515	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	1	58	theme	different	441:449	arg1	NPs					512:514	PE-CS-Au NPs	503:514	PE-CS-Au NPs	503:514	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	1	59	theme	-loaded	268:274	arg1	system					290:295	Punica granatum L. extract (PE)-loaded drug delivery system	237:295	Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability	237:341	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	2	60	theme	chemistry	602:610	arg1	approach					612:619	green chemistry approach	596:619	green chemistry approach	596:619	METHODS PE-conjugated CS-Au nanoparticles (PE-CS-Au NPs) were synthesized via green chemistry approach.
34354349	9	61	theme	PE-CS-Au	1497:1504	arg1	NPs					1506:1508	PE-CS-Au NPs	1497:1508	PE-CS-Au NPs	1497:1508	It is worth noting that crosslinking and incorporation of PE-CS-Au NPs improved thermal stability and mechanical properties of the obtained NFs.
34354349	1	62	theme	PE-CS-gold	477:486	arg1	nanoparticles					488:500	biosynthesized PE-CS-gold nanoparticles	462:500	biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs)	462:515	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	1	62	theme	PE-CS-gold	477:486	arg1	NPs					512:514	PE-CS-Au NPs	503:514	PE-CS-Au NPs	503:514	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	13	63	theme	populations	2263:2273	arg1	proliferation					2235:2247	proliferation	2235:2247	proliferation	2235:2247	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	13	63	theme	populations	2263:2273	arg1	growth					2224:2229	growth	2224:2229	growth	2224:2229	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	13	63	theme	populations	2263:2273	arg1	adhesion					2214:2221	cell adhesion	2209:2221	cell adhesion	2209:2221	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	12	64	theme	highest	1907:1913	arg1	viability					1915:1923	the highest viability	1903:1923	the highest viability (123%)	1903:1930	The human fibroblasts (HFF-1) showed the highest viability (123%) by seeding over the PVA NFs mats containing 0.9% PE-CS-Au NPs.
34354349	12	64	theme	highest	1907:1913	arg1	%					1929:1929	123%	1926:1929	123%	1926:1929	The human fibroblasts (HFF-1) showed the highest viability (123%) by seeding over the PVA NFs mats containing 0.9% PE-CS-Au NPs.
34354349	11	65	theme	antibacterial	1757:1769	arg1	proportional					1805:1816	proportional	1805:1816	proportional	1805:1816	The antibacterial activity was found to be directly proportional to the amount of the incorporated PE-CS-Au NPs.
34354349	11	65	theme	antibacterial	1757:1769	arg1	activity					1771:1778	The antibacterial activity	1753:1778	The antibacterial activity	1753:1778	The antibacterial activity was found to be directly proportional to the amount of the incorporated PE-CS-Au NPs.
34354349	9	66	theme	NPs	1506:1508	arg1	crosslinking					1463:1474	crosslinking	1463:1474	crosslinking	1463:1474	It is worth noting that crosslinking and incorporation of PE-CS-Au NPs improved thermal stability and mechanical properties of the obtained NFs.
34354349	9	66	theme	NPs	1506:1508	arg1	incorporation					1480:1492	incorporation	1480:1492	incorporation	1480:1492	It is worth noting that crosslinking and incorporation of PE-CS-Au NPs improved thermal stability and mechanical properties of the obtained NFs.
34354349	1	67	theme	PVA	393:395	arg1	nanofibers					397:406	crosslinked PVA nanofibers	381:406	crosslinked PVA nanofibers (NFs)	381:412	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	1	67	theme	PVA	393:395	arg1	NFs					409:411	NFs	409:411	NFs	409:411	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	12	68	contain	containing	1965:1974	arg1	mats					1960:1963	the PVA NFs mats	1948:1963	the PVA NFs mats containing 0.9% PE-CS-Au NPs	1948:1992	The human fibroblasts (HFF-1) showed the highest viability (123%) by seeding over the PVA NFs mats containing 0.9% PE-CS-Au NPs.
34354349	12	68	contain	containing	1965:1974	arg2	NPs					1990:1992	0.9% PE-CS-Au NPs	1976:1992	0.9% PE-CS-Au NPs	1976:1992	The human fibroblasts (HFF-1) showed the highest viability (123%) by seeding over the PVA NFs mats containing 0.9% PE-CS-Au NPs.
34354349	13	69	theme	antibacterial	2119:2131	arg1	composites					2064:2073	the electrospun PVA NFs composites	2040:2073	the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs	2040:2102	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	13	69	theme	antibacterial	2119:2131	arg1	agents					2133:2138	antibacterial agents	2119:2138	antibacterial agents against antibiotic-resistant bacteria	2119:2176	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	7	70	theme	release	1181:1187	arg1	kinetics					1189:1196	release kinetics	1181:1196	release kinetics	1181:1196	Swelling capacity, biodegradability, PE release profiles, release kinetics, antibacterial and cell biocompatibility were also demonstrated.
34354349	13	71	theme	PVA	2056:2058	arg1	scaffolds					2195:2203	suitable scaffolds	2186:2203	suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations	2186:2273	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	13	71	theme	PVA	2056:2058	arg1	composites					2064:2073	the electrospun PVA NFs composites	2040:2073	the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs	2040:2102	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	13	71	theme	PVA	2056:2058	arg1	agents					2133:2138	antibacterial agents	2119:2138	antibacterial agents against antibiotic-resistant bacteria	2119:2176	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	12	72	theme	PE-CS-Au	1981:1988	arg1	NPs					1990:1992	0.9% PE-CS-Au NPs	1976:1992	0.9% PE-CS-Au NPs	1976:1992	The human fibroblasts (HFF-1) showed the highest viability (123%) by seeding over the PVA NFs mats containing 0.9% PE-CS-Au NPs.
34354349	8	73	theme	PE-CS-Au	1288:1295	arg1	NPs					1297:1299	PE-CS-Au NPs	1288:1299	PE-CS-Au NPs	1288:1299	RESULTS By incorporating PE-CS-Au NPs at 0.6% and 0.9%, the diameters of the nanofibers decreased from 295.7±83.1 nm in neat PVA to 165.6±43.4 and 147.8±42.7 nm, respectively.
34354349	13	74	theme	obtained	2010:2017	arg1	results					2019:2025	The obtained results	2006:2025	The obtained results	2006:2025	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	3	75	theme	PE-CS-Au	639:646	arg1	NPs					648:650	PE-CS-Au NPs	639:650	PE-CS-Au NPs	639:650	The formation of PE-CS-Au NPs was confirmed by UV spectroscopy, DLS, SEM and STEM.
34354349	13	76	theme	cell	2209:2212	arg1	adhesion					2214:2221	cell adhesion	2209:2221	cell adhesion	2209:2221	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	4	77	from	ratios	789:794	arg1	solution					767:774	polyvinyl alcohol (PVA) solution	743:774	polyvinyl alcohol (PVA) solution at different ratios	743:794	PE-CS-Au NPs were then dispersed into polyvinyl alcohol (PVA) solution at different ratios, where the optimized ratios were selected for electrospinning and further studies.
34354349	6	78	dep	morphology	980:989	arg1	The					976:978	The	976:978	The	976:978	The morphology, chemical compositions, thermal stability and mechanical properties of PE-CS-Au NPs loaded NFs were evaluated by SEM, FTIR and DSC.
34354349	13	79	theme	%	2089:2089	arg1	NPs					2100:2102	0.9% PE-CS-Au NPs	2086:2102	0.9% PE-CS-Au NPs	2086:2102	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	12	80	theme	human	1870:1874	arg1	HFF-1					1889:1893	HFF-1	1889:1893	HFF-1	1889:1893	The human fibroblasts (HFF-1) showed the highest viability (123%) by seeding over the PVA NFs mats containing 0.9% PE-CS-Au NPs.
34354349	12	80	theme	human	1870:1874	arg1	fibroblasts					1876:1886	The human fibroblasts	1866:1886	The human fibroblasts (HFF-1)	1866:1894	The human fibroblasts (HFF-1) showed the highest viability (123%) by seeding over the PVA NFs mats containing 0.9% PE-CS-Au NPs.
34354349	5	81	theme	PE-CS-Au	895:902	arg1	NPs					904:906	PE-CS-Au NPs	895:906	PE-CS-Au NPs loaded PVA nanofibers (NFs)	895:934	Crosslinking of PE-CS-Au NPs loaded PVA nanofibers (NFs) was performed via glutaraldehyde vapor.
34354349	6	82	theme	chemical	992:999	arg1	compositions					1001:1012	chemical compositions	992:1012	chemical compositions	992:1012	The morphology, chemical compositions, thermal stability and mechanical properties of PE-CS-Au NPs loaded NFs were evaluated by SEM, FTIR and DSC.
34354349	12	83	theme	0.9	1976:1978	arg1	%					1979:1979	%	1979:1979	%	1979:1979	The human fibroblasts (HFF-1) showed the highest viability (123%) by seeding over the PVA NFs mats containing 0.9% PE-CS-Au NPs.
34354349	1	84	link	crosslinked	381:391	arg1	nanofibers					397:406	crosslinked PVA nanofibers	381:406	crosslinked PVA nanofibers (NFs)	381:412	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	1	84	link	crosslinked	381:391	arg1	NFs					409:411	NFs	409:411	NFs	409:411	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	0	85	theme	Punica	105:110	arg1	Extract					124:130	Punica granatum L. Extract	105:130	Punica granatum L. Extract	105:130	Chitosan/Gold Hybrid Nanoparticles Enriched Electrospun PVA Nanofibrous Mats for the Topical Delivery of Punica granatum L. Extract: Synthesis, Characterization, Biocompatibility and Antibacterial Properties.
34354349	13	86	used	used	2111:2114	arg2	agents					2133:2138	antibacterial agents	2119:2138	antibacterial agents against antibiotic-resistant bacteria	2119:2176	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	13	86	used	used	2111:2114	arg2	composites					2064:2073	the electrospun PVA NFs composites	2040:2073	the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs	2040:2102	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	13	86	used	used	2111:2114	arg2	scaffolds					2195:2203	suitable scaffolds	2186:2203	suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations	2186:2273	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	2	87	theme	CS-Au	540:544	arg1	NPs					570:572	PE-CS-Au NPs	561:572	PE-CS-Au NPs	561:572	METHODS PE-conjugated CS-Au nanoparticles (PE-CS-Au NPs) were synthesized via green chemistry approach.
34354349	2	87	theme	CS-Au	540:544	arg1	nanoparticles					546:558	PE-conjugated CS-Au nanoparticles	526:558	METHODS PE-conjugated CS-Au nanoparticles (PE-CS-Au NPs)	518:573	METHODS PE-conjugated CS-Au nanoparticles (PE-CS-Au NPs) were synthesized via green chemistry approach.
34354349	9	88	theme	thermal	1519:1525	arg1	stability					1527:1535	thermal stability	1519:1535	thermal stability	1519:1535	It is worth noting that crosslinking and incorporation of PE-CS-Au NPs improved thermal stability and mechanical properties of the obtained NFs.
34354349	0	89	theme	L.	121:122	arg1	Extract					124:130	Punica granatum L. Extract	105:130	Punica granatum L. Extract	105:130	Chitosan/Gold Hybrid Nanoparticles Enriched Electrospun PVA Nanofibrous Mats for the Topical Delivery of Punica granatum L. Extract: Synthesis, Characterization, Biocompatibility and Antibacterial Properties.
34354349	8	90	dep	RESULTS	1263:1269	arg1	decreased					1351:1359	decreased	1351:1359	decreased	1351:1359	RESULTS By incorporating PE-CS-Au NPs at 0.6% and 0.9%, the diameters of the nanofibers decreased from 295.7±83.1 nm in neat PVA to 165.6±43.4 and 147.8±42.7 nm, respectively.
34354349	6	91	theme	NFs	1082:1084	arg1	properties					1048:1057	mechanical properties	1037:1057	mechanical properties	1037:1057	The morphology, chemical compositions, thermal stability and mechanical properties of PE-CS-Au NPs loaded NFs were evaluated by SEM, FTIR and DSC.
34354349	6	91	theme	NFs	1082:1084	arg1	morphology					980:989	morphology	980:989	morphology	980:989	The morphology, chemical compositions, thermal stability and mechanical properties of PE-CS-Au NPs loaded NFs were evaluated by SEM, FTIR and DSC.
34354349	6	91	theme	NFs	1082:1084	arg1	compositions					1001:1012	chemical compositions	992:1012	chemical compositions	992:1012	The morphology, chemical compositions, thermal stability and mechanical properties of PE-CS-Au NPs loaded NFs were evaluated by SEM, FTIR and DSC.
34354349	6	91	theme	NFs	1082:1084	arg1	stability					1023:1031	thermal stability	1015:1031	thermal stability	1015:1031	The morphology, chemical compositions, thermal stability and mechanical properties of PE-CS-Au NPs loaded NFs were evaluated by SEM, FTIR and DSC.
34354349	13	92	theme	PE-CS-Au	2091:2098	arg1	NPs					2100:2102	0.9% PE-CS-Au NPs	2086:2102	0.9% PE-CS-Au NPs	2086:2102	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	0	93	theme	Hybrid	14:19	arg1	Nanoparticles					21:33	Chitosan/Gold Hybrid Nanoparticles	0:33	Chitosan/Gold Hybrid Nanoparticles	0:33	Chitosan/Gold Hybrid Nanoparticles Enriched Electrospun PVA Nanofibrous Mats for the Topical Delivery of Punica granatum L. Extract: Synthesis, Characterization, Biocompatibility and Antibacterial Properties.
34354349	9	94	theme	mechanical	1541:1550	arg1	properties					1552:1561	mechanical properties	1541:1561	mechanical properties of the obtained NFs	1541:1581	It is worth noting that crosslinking and incorporation of PE-CS-Au NPs improved thermal stability and mechanical properties of the obtained NFs.
34354349	6	95	theme	NPs	1071:1073	arg1	NFs					1082:1084	PE-CS-Au NPs loaded NFs	1062:1084	PE-CS-Au NPs loaded NFs	1062:1084	The morphology, chemical compositions, thermal stability and mechanical properties of PE-CS-Au NPs loaded NFs were evaluated by SEM, FTIR and DSC.
34354349	11	96	theme	PE-CS-Au	1852:1859	arg1	NPs					1861:1863	the incorporated PE-CS-Au NPs	1835:1863	the incorporated PE-CS-Au NPs	1835:1863	The antibacterial activity was found to be directly proportional to the amount of the incorporated PE-CS-Au NPs.
34354349	4	97	theme	polyvinyl	743:751	arg1	PVA					762:764	PVA	762:764	PVA	762:764	PE-CS-Au NPs were then dispersed into polyvinyl alcohol (PVA) solution at different ratios, where the optimized ratios were selected for electrospinning and further studies.
34354349	4	97	theme	polyvinyl	743:751	arg1	alcohol					753:759	polyvinyl alcohol	743:759	polyvinyl alcohol (PVA) solution at different ratios	743:794	PE-CS-Au NPs were then dispersed into polyvinyl alcohol (PVA) solution at different ratios, where the optimized ratios were selected for electrospinning and further studies.
34354349	10	98	theme	diffusion	1639:1647	arg1	value					1662:1666	n value ˂0.5	1660:1671	n value ˂0.5	1660:1671	The release of PE from NFs was controlled by a Fickian diffusion mechanism (n value ˂0.5), whereas Higuchi was the mathematical model which could describe this release.
34354349	10	98	theme	diffusion	1639:1647	arg1	mechanism					1649:1657	a Fickian diffusion mechanism	1629:1657	a Fickian diffusion mechanism (n value ˂0.5)	1629:1672	The release of PE from NFs was controlled by a Fickian diffusion mechanism (n value ˂0.5), whereas Higuchi was the mathematical model which could describe this release.
34354349	1	99	theme	better	300:305	arg1	impact					307:312	better impact	300:312	better impact	300:312	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	9	100	theme	obtained	1570:1577	arg1	NFs					1579:1581	the obtained NFs	1566:1581	the obtained NFs	1566:1581	It is worth noting that crosslinking and incorporation of PE-CS-Au NPs improved thermal stability and mechanical properties of the obtained NFs.
34354349	6	101	theme	mechanical	1037:1046	arg1	properties					1048:1057	mechanical properties	1037:1057	mechanical properties	1037:1057	The morphology, chemical compositions, thermal stability and mechanical properties of PE-CS-Au NPs loaded NFs were evaluated by SEM, FTIR and DSC.
34354349	1	102	theme	PE-CS-Au	503:510	arg1	nanoparticles					488:500	biosynthesized PE-CS-gold nanoparticles	462:500	biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs)	462:515	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	1	102	theme	PE-CS-Au	503:510	arg1	NPs					512:514	PE-CS-Au NPs	503:514	PE-CS-Au NPs	503:514	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	10	103	from	NFs	1607:1609	arg1	release					1588:1594	The release	1584:1594	The release of PE from NFs	1584:1609	The release of PE from NFs was controlled by a Fickian diffusion mechanism (n value ˂0.5), whereas Higuchi was the mathematical model which could describe this release.
34354349	4	104	theme	optimized	807:815	arg1	ratios					817:822	the optimized ratios	803:822	the optimized ratios	803:822	PE-CS-Au NPs were then dispersed into polyvinyl alcohol (PVA) solution at different ratios, where the optimized ratios were selected for electrospinning and further studies.
34354349	13	105	theme	electrospun	2044:2054	arg1	scaffolds					2195:2203	suitable scaffolds	2186:2203	suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations	2186:2273	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	13	105	theme	electrospun	2044:2054	arg1	composites					2064:2073	the electrospun PVA NFs composites	2040:2073	the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs	2040:2102	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	13	105	theme	electrospun	2044:2054	arg1	agents					2133:2138	antibacterial agents	2119:2138	antibacterial agents against antibiotic-resistant bacteria	2119:2176	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	1	106	theme	applicability	329:341	arg1	system					290:295	Punica granatum L. extract (PE)-loaded drug delivery system	237:295	Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability	237:341	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	1	107	theme	L.	253:254	arg1	system					290:295	Punica granatum L. extract (PE)-loaded drug delivery system	237:295	Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability	237:341	PURPOSE Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports the use of crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts of biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).
34354349	13	108	contain	containing	2075:2084	arg1	scaffolds					2195:2203	suitable scaffolds	2186:2203	suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations	2186:2273	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	13	108	contain	containing	2075:2084	arg2	NPs					2100:2102	0.9% PE-CS-Au NPs	2086:2102	0.9% PE-CS-Au NPs	2086:2102	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	13	108	contain	containing	2075:2084	arg1	composites					2064:2073	the electrospun PVA NFs composites	2040:2073	the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs	2040:2102	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	13	108	contain	containing	2075:2084	arg1	agents					2133:2138	antibacterial agents	2119:2138	antibacterial agents against antibiotic-resistant bacteria	2119:2176	CONCLUSION The obtained results suggest that the electrospun PVA NFs composites containing 0.9% PE-CS-Au NPs can be used as antibacterial agents against antibiotic-resistant bacteria, and as suitable scaffolds for cell adhesion, growth and proliferation of fibroblast populations.
34354349	10	109	dep	controlled	1615:1624	arg1	whereas					1675:1681	whereas	1675:1681	whereas	1675:1681	The release of PE from NFs was controlled by a Fickian diffusion mechanism (n value ˂0.5), whereas Higuchi was the mathematical model which could describe this release.
34354349	7	110	theme	PE	1160:1161	arg1	profiles					1171:1178	PE release profiles	1160:1178	PE release profiles	1160:1178	Swelling capacity, biodegradability, PE release profiles, release kinetics, antibacterial and cell biocompatibility were also demonstrated.
34420495	6	0	with	combination	869:879	arg1	probiotics					886:895	probiotics	886:895	probiotics	886:895	The prebiotic fructo-oligosaccharides (FOS) and its combination with probiotics reduced the residual fitness of the E. coli strain in some farms.
34420495	12	1	from	studies	2043:2049	arg1	crucial					2025:2031	crucial	2025:2031	crucial	2025:2031	However, evaluation of farm metadata (husbandry, feed, management) will be crucial in future studies to determine a tailor-made solution for combinations of pre- and probiotics for individual farms.
34420495	2	2	from	farms	281:285	arg1	slurries					253:260	Sow faecal slurries	242:260	Sow faecal slurries from 20 German pig farms	242:285	Sow faecal slurries from 20 German pig farms were anaerobically incubated with pre- and probiotics or their combinations together with pathogenic strains that are of interest in pig production.
34420495	2	3	theme	pig	277:279	arg1	farms					281:285	20 German pig farms	267:285	20 German pig farms	267:285	Sow faecal slurries from 20 German pig farms were anaerobically incubated with pre- and probiotics or their combinations together with pathogenic strains that are of interest in pig production.
34420495	2	4	theme	faecal	246:251	arg1	slurries					253:260	Sow faecal slurries	242:260	Sow faecal slurries from 20 German pig farms	242:285	Sow faecal slurries from 20 German pig farms were anaerobically incubated with pre- and probiotics or their combinations together with pathogenic strains that are of interest in pig production.
34420495	9	5	theme	prebiotics	1375:1384	arg1	application					1360:1370	single application	1353:1370	single application of prebiotics and their combination with probiotics	1353:1422	The effects of pre- and probiotics on the residual fitness of the C. difficile strain were less pronounced, but distinct differences between single application of prebiotics and their combination with probiotics were observed.
34420495	9	6	theme	single	1353:1358	arg1	application					1360:1370	single application	1353:1370	single application of prebiotics and their combination with probiotics	1353:1422	The effects of pre- and probiotics on the residual fitness of the C. difficile strain were less pronounced, but distinct differences between single application of prebiotics and their combination with probiotics were observed.
34420495	3	7	theme	antibiotic	505:514	arg1	mixtures					516:523	antibiotic mixtures	505:523	antibiotic mixtures allowing only growth of the specific pathogen	505:569	Aliquots of these slurries were then incubated with media containing antibiotic mixtures allowing only growth of the specific pathogen.
34420495	13	8	theme	growth	2259:2264	arg1	validation					2233:2242	validation	2233:2242	validation of unambiguous growth	2233:2264	Also, refinement of the ex vivo assay in terms of on-farm processing of samples and validation of unambiguous growth for pathogenic strains from individual farms should be addressed.
34420495	13	8	theme	growth	2259:2264	arg1	processing					2207:2216	on-farm processing	2199:2216	on-farm processing of samples	2199:2227	Also, refinement of the ex vivo assay in terms of on-farm processing of samples and validation of unambiguous growth for pathogenic strains from individual farms should be addressed.
34420495	2	9	theme	German	270:275	arg1	farms					281:285	20 German pig farms	267:285	20 German pig farms	267:285	Sow faecal slurries from 20 German pig farms were anaerobically incubated with pre- and probiotics or their combinations together with pathogenic strains that are of interest in pig production.
34420495	1	10	theme	concept	141:147	arg1	part					131:134	part	131:134	part of a concept to determine potential tailor-made combinations of pre- and probiotics for individual farms	131:239	A novel rapid ex vivo assay was developed as part of a concept to determine potential tailor-made combinations of pre- and probiotics for individual farms.
34420495	1	10	theme	concept	141:147	arg1	assay					108:112	A novel rapid ex vivo assay	86:112	A novel rapid ex vivo assay	86:112	A novel rapid ex vivo assay was developed as part of a concept to determine potential tailor-made combinations of pre- and probiotics for individual farms.
34420495	6	11	theme	strain	941:946	arg1	fitness					918:924	the residual fitness	905:924	the residual fitness of the E. coli strain in some farms	905:960	The prebiotic fructo-oligosaccharides (FOS) and its combination with probiotics reduced the residual fitness of the E. coli strain in some farms.
34420495	3	12	theme	pathogen	562:569	arg1	growth					539:544	only growth	534:544	only growth of the specific pathogen	534:569	Aliquots of these slurries were then incubated with media containing antibiotic mixtures allowing only growth of the specific pathogen.
34420495	12	13	theme	future	2036:2041	arg1	studies					2043:2049	future studies	2036:2049	future studies	2036:2049	However, evaluation of farm metadata (husbandry, feed, management) will be crucial in future studies to determine a tailor-made solution for combinations of pre- and probiotics for individual farms.
34420495	6	14	theme	E.	933:934	arg1	strain					941:946	the E. coli strain	929:946	the E. coli strain	929:946	The prebiotic fructo-oligosaccharides (FOS) and its combination with probiotics reduced the residual fitness of the E. coli strain in some farms.
34420495	12	15	from	crucial	2025:2031	arg1	studies					2043:2049	future studies	2036:2049	future studies	2036:2049	However, evaluation of farm metadata (husbandry, feed, management) will be crucial in future studies to determine a tailor-made solution for combinations of pre- and probiotics for individual farms.
34420495	1	16	theme	tailor-made	172:182	arg1	combinations					184:195	potential tailor-made combinations	162:195	potential tailor-made combinations of pre- and probiotics for individual farms	162:239	A novel rapid ex vivo assay was developed as part of a concept to determine potential tailor-made combinations of pre- and probiotics for individual farms.
34420495	10	17	theme	C.	1566:1567	arg1	strain					1579:1584	the C. difficile strain	1562:1584	the C. difficile strain	1562:1584	It was concluded that the initial composition of the microbiota in the samples was more determinative for incubations with the C. difficile strain than for incubations with the E. coli strain, as the presumed fermentation of prebiotic products showed less influence on the fitness of the C. difficile strain.
34420495	11	18	theme	dependent	1753:1761	arg1	differences					1763:1773	Farm dependent differences	1748:1773	Farm dependent differences	1748:1773	Farm dependent differences were pronounced for both pathogenic strains and therefore, this novel screening method offers a promising approach for pre-selecting pre- and probiotics for individual farms.
34420495	10	19	theme	difficile	1730:1738	arg1	strain					1740:1745	the C. difficile strain	1723:1745	the C. difficile strain	1723:1745	It was concluded that the initial composition of the microbiota in the samples was more determinative for incubations with the C. difficile strain than for incubations with the E. coli strain, as the presumed fermentation of prebiotic products showed less influence on the fitness of the C. difficile strain.
34420495	13	20	theme	on-farm	2199:2205	arg1	processing					2207:2216	on-farm processing	2199:2216	on-farm processing of samples	2199:2227	Also, refinement of the ex vivo assay in terms of on-farm processing of samples and validation of unambiguous growth for pathogenic strains from individual farms should be addressed.
34420495	6	21	theme	residual	909:916	arg1	fitness					918:924	the residual fitness	905:924	the residual fitness of the E. coli strain in some farms	905:960	The prebiotic fructo-oligosaccharides (FOS) and its combination with probiotics reduced the residual fitness of the E. coli strain in some farms.
34420495	6	22	dep	E.	933:934	arg1	coli					936:939	coli	936:939	coli	936:939	The prebiotic fructo-oligosaccharides (FOS) and its combination with probiotics reduced the residual fitness of the E. coli strain in some farms.
34420495	7	23	theme	other	1003:1007	arg1	farms					1009:1013	other farms	1003:1013	other farms where FOS increased the fitness of the E. coli strain	1003:1067	However, notable exceptions occurred in other farms where FOS increased the fitness of the E. coli strain.
34420495	4	24	used	used	610:613	arg2	time					601:604	lag time	597:604	lag time	597:604	Growth was monitored and lag time was used to determine the residual fitness of the pathogenic strains.
34420495	13	25	theme	processing	2207:2216	arg1	terms					2190:2194	terms	2190:2194	terms of on-farm processing of samples and validation of unambiguous growth	2190:2264	Also, refinement of the ex vivo assay in terms of on-farm processing of samples and validation of unambiguous growth for pathogenic strains from individual farms should be addressed.
34420495	5	26	theme	Escherichia	724:734	arg1	coli-					736:740	an Escherichia coli-	721:740	an Escherichia coli-	721:740	The background growth could be inhibited for an Escherichia coli- and a Clostridium difficile- but not for a Clostridium perfringens strain.
34420495	9	27	theme	pre-	1227:1230	arg1	pronounced					1308:1317	pronounced	1308:1317	pronounced	1308:1317	The effects of pre- and probiotics on the residual fitness of the C. difficile strain were less pronounced, but distinct differences between single application of prebiotics and their combination with probiotics were observed.
34420495	9	27	theme	pre-	1227:1230	arg1	effects					1216:1222	The effects	1212:1222	The effects of pre- and probiotics on the residual fitness of the C. difficile strain	1212:1296	The effects of pre- and probiotics on the residual fitness of the C. difficile strain were less pronounced, but distinct differences between single application of prebiotics and their combination with probiotics were observed.
34420495	11	28	theme	individual	1932:1941	arg1	farms					1943:1947	individual farms	1932:1947	individual farms	1932:1947	Farm dependent differences were pronounced for both pathogenic strains and therefore, this novel screening method offers a promising approach for pre-selecting pre- and probiotics for individual farms.
34420495	13	29	theme	assay	2181:2185	arg1	refinement					2155:2164	refinement	2155:2164	refinement of the ex vivo assay in terms of on-farm processing of samples and validation of unambiguous growth for pathogenic strains from individual farms	2155:2309	Also, refinement of the ex vivo assay in terms of on-farm processing of samples and validation of unambiguous growth for pathogenic strains from individual farms should be addressed.
34420495	3	30	theme	specific	553:560	arg1	pathogen					562:569	the specific pathogen	549:569	the specific pathogen	549:569	Aliquots of these slurries were then incubated with media containing antibiotic mixtures allowing only growth of the specific pathogen.
34420495	13	31	dep	ex	2173:2174	arg1	vivo					2176:2179	vivo	2176:2179	vivo	2176:2179	Also, refinement of the ex vivo assay in terms of on-farm processing of samples and validation of unambiguous growth for pathogenic strains from individual farms should be addressed.
34420495	4	32	theme	strains	667:673	arg1	fitness					641:647	the residual fitness	628:647	the residual fitness of the pathogenic strains	628:673	Growth was monitored and lag time was used to determine the residual fitness of the pathogenic strains.
34420495	9	33	theme	probiotics	1236:1245	arg1	pronounced					1308:1317	pronounced	1308:1317	pronounced	1308:1317	The effects of pre- and probiotics on the residual fitness of the C. difficile strain were less pronounced, but distinct differences between single application of prebiotics and their combination with probiotics were observed.
34420495	9	33	theme	probiotics	1236:1245	arg1	effects					1216:1222	The effects	1212:1222	The effects of pre- and probiotics on the residual fitness of the C. difficile strain	1212:1296	The effects of pre- and probiotics on the residual fitness of the C. difficile strain were less pronounced, but distinct differences between single application of prebiotics and their combination with probiotics were observed.
34420495	2	34	from	interest	408:415	arg1	production					424:433	pig production	420:433	pig production	420:433	Sow faecal slurries from 20 German pig farms were anaerobically incubated with pre- and probiotics or their combinations together with pathogenic strains that are of interest in pig production.
34420495	1	35	dep	ex	100:101	arg1	vivo					103:106	vivo	103:106	vivo	103:106	A novel rapid ex vivo assay was developed as part of a concept to determine potential tailor-made combinations of pre- and probiotics for individual farms.
34420495	10	36	from	composition	1473:1483	arg1	samples					1510:1516	the samples	1506:1516	the samples	1506:1516	It was concluded that the initial composition of the microbiota in the samples was more determinative for incubations with the C. difficile strain than for incubations with the E. coli strain, as the presumed fermentation of prebiotic products showed less influence on the fitness of the C. difficile strain.
34420495	1	37	dep	novel	88:92	arg1	rapid					94:98	rapid	94:98	rapid	94:98	A novel rapid ex vivo assay was developed as part of a concept to determine potential tailor-made combinations of pre- and probiotics for individual farms.
34420495	13	38	from	farms	2305:2309	arg1	strains					2281:2287	pathogenic strains	2270:2287	pathogenic strains from individual farms	2270:2309	Also, refinement of the ex vivo assay in terms of on-farm processing of samples and validation of unambiguous growth for pathogenic strains from individual farms should be addressed.
34420495	13	39	theme	ex	2173:2174	arg1	assay					2181:2185	the ex vivo assay	2169:2185	the ex vivo assay in terms of on-farm processing of samples and validation of unambiguous growth for pathogenic strains from individual farms	2169:2309	Also, refinement of the ex vivo assay in terms of on-farm processing of samples and validation of unambiguous growth for pathogenic strains from individual farms should be addressed.
34420495	13	40	from	refinement	2155:2164	arg1	terms					2190:2194	terms	2190:2194	terms of on-farm processing of samples and validation of unambiguous growth	2190:2264	Also, refinement of the ex vivo assay in terms of on-farm processing of samples and validation of unambiguous growth for pathogenic strains from individual farms should be addressed.
34420495	11	41	theme	novel	1839:1843	arg1	method					1855:1860	this novel screening method	1834:1860	this novel screening method	1834:1860	Farm dependent differences were pronounced for both pathogenic strains and therefore, this novel screening method offers a promising approach for pre-selecting pre- and probiotics for individual farms.
34420495	0	42	theme	Novel	0:4	arg1	assay					24:28	Novel ex vivo screening assay	0:28	Novel ex vivo screening assay	0:28	Novel ex vivo screening assay to preselect farm specific pre- and probiotics in pigs.
34420495	5	43	theme	Clostridium	785:795	arg1	strain					809:814	a Clostridium perfringens strain	783:814	a Clostridium perfringens strain	783:814	The background growth could be inhibited for an Escherichia coli- and a Clostridium difficile- but not for a Clostridium perfringens strain.
34420495	9	44	theme	difficile	1281:1289	arg1	strain					1291:1296	the C. difficile strain	1274:1296	the C. difficile strain	1274:1296	The effects of pre- and probiotics on the residual fitness of the C. difficile strain were less pronounced, but distinct differences between single application of prebiotics and their combination with probiotics were observed.
34420495	10	45	from	microbiota	1492:1501	arg1	samples					1510:1516	the samples	1506:1516	the samples	1506:1516	It was concluded that the initial composition of the microbiota in the samples was more determinative for incubations with the C. difficile strain than for incubations with the E. coli strain, as the presumed fermentation of prebiotic products showed less influence on the fitness of the C. difficile strain.
34420495	12	46	theme	probiotics	2116:2125	arg1	combinations					2091:2102	combinations	2091:2102	combinations of pre- and probiotics	2091:2125	However, evaluation of farm metadata (husbandry, feed, management) will be crucial in future studies to determine a tailor-made solution for combinations of pre- and probiotics for individual farms.
34420495	2	47	theme	pathogenic	377:386	arg1	strains					388:394	pathogenic strains	377:394	pathogenic strains that are of interest in pig production	377:433	Sow faecal slurries from 20 German pig farms were anaerobically incubated with pre- and probiotics or their combinations together with pathogenic strains that are of interest in pig production.
34420495	5	48	theme	Clostridium	748:758	arg1	difficile-					760:769	a Clostridium difficile-	746:769	a Clostridium difficile-	746:769	The background growth could be inhibited for an Escherichia coli- and a Clostridium difficile- but not for a Clostridium perfringens strain.
34420495	10	49	with	incubations	1545:1555	arg1	strain					1579:1584	the C. difficile strain	1562:1584	the C. difficile strain	1562:1584	It was concluded that the initial composition of the microbiota in the samples was more determinative for incubations with the C. difficile strain than for incubations with the E. coli strain, as the presumed fermentation of prebiotic products showed less influence on the fitness of the C. difficile strain.
34420495	3	50	contain	containing	494:503	arg2	mixtures					516:523	antibiotic mixtures	505:523	antibiotic mixtures allowing only growth of the specific pathogen	505:569	Aliquots of these slurries were then incubated with media containing antibiotic mixtures allowing only growth of the specific pathogen.
34420495	3	50	contain	containing	494:503	arg1	media					488:492	media	488:492	media containing antibiotic mixtures allowing only growth of the specific pathogen	488:569	Aliquots of these slurries were then incubated with media containing antibiotic mixtures allowing only growth of the specific pathogen.
34420495	9	51	with	combination	1396:1406	arg1	probiotics					1413:1422	probiotics	1413:1422	probiotics	1413:1422	The effects of pre- and probiotics on the residual fitness of the C. difficile strain were less pronounced, but distinct differences between single application of prebiotics and their combination with probiotics were observed.
34420495	12	52	theme	individual	2131:2140	arg1	farms					2142:2146	individual farms	2131:2146	individual farms	2131:2146	However, evaluation of farm metadata (husbandry, feed, management) will be crucial in future studies to determine a tailor-made solution for combinations of pre- and probiotics for individual farms.
34420495	9	53	with	prebiotics	1375:1384	arg1	probiotics					1413:1422	probiotics	1413:1422	probiotics	1413:1422	The effects of pre- and probiotics on the residual fitness of the C. difficile strain were less pronounced, but distinct differences between single application of prebiotics and their combination with probiotics were observed.
34420495	3	54	theme	slurries	454:461	arg1	Aliquots					436:443	Aliquots	436:443	Aliquots of these slurries	436:461	Aliquots of these slurries were then incubated with media containing antibiotic mixtures allowing only growth of the specific pathogen.
34420495	10	55	theme	initial	1465:1471	arg1	composition					1473:1483	the initial composition	1461:1483	the initial composition of the microbiota in the samples	1461:1516	It was concluded that the initial composition of the microbiota in the samples was more determinative for incubations with the C. difficile strain than for incubations with the E. coli strain, as the presumed fermentation of prebiotic products showed less influence on the fitness of the C. difficile strain.
34420495	10	55	theme	initial	1465:1471	arg1	determinative					1527:1539	determinative	1527:1539	determinative	1527:1539	It was concluded that the initial composition of the microbiota in the samples was more determinative for incubations with the C. difficile strain than for incubations with the E. coli strain, as the presumed fermentation of prebiotic products showed less influence on the fitness of the C. difficile strain.
34420495	11	56	theme	promising	1871:1879	arg1	approach					1881:1888	a promising approach	1869:1888	a promising approach for pre-selecting pre- and probiotics for individual farms	1869:1947	Farm dependent differences were pronounced for both pathogenic strains and therefore, this novel screening method offers a promising approach for pre-selecting pre- and probiotics for individual farms.
34420495	12	57	theme	pre-	2107:2110	arg1	combinations					2091:2102	combinations	2091:2102	combinations of pre- and probiotics	2091:2125	However, evaluation of farm metadata (husbandry, feed, management) will be crucial in future studies to determine a tailor-made solution for combinations of pre- and probiotics for individual farms.
34420495	1	58	theme	pre-	200:203	arg1	combinations					184:195	potential tailor-made combinations	162:195	potential tailor-made combinations of pre- and probiotics for individual farms	162:239	A novel rapid ex vivo assay was developed as part of a concept to determine potential tailor-made combinations of pre- and probiotics for individual farms.
34420495	12	59	theme	farm	1973:1976	arg1	metadata					1978:1985	farm metadata	1973:1985	farm metadata (husbandry, feed, management)	1973:2015	However, evaluation of farm metadata (husbandry, feed, management) will be crucial in future studies to determine a tailor-made solution for combinations of pre- and probiotics for individual farms.
34420495	10	60	with	incubations	1595:1605	arg1	strain					1624:1629	the E. coli strain	1612:1629	the E. coli strain	1612:1629	It was concluded that the initial composition of the microbiota in the samples was more determinative for incubations with the C. difficile strain than for incubations with the E. coli strain, as the presumed fermentation of prebiotic products showed less influence on the fitness of the C. difficile strain.
34420495	10	61	theme	microbiota	1492:1501	arg1	composition					1473:1483	the initial composition	1461:1483	the initial composition of the microbiota in the samples	1461:1516	It was concluded that the initial composition of the microbiota in the samples was more determinative for incubations with the C. difficile strain than for incubations with the E. coli strain, as the presumed fermentation of prebiotic products showed less influence on the fitness of the C. difficile strain.
34420495	10	61	theme	microbiota	1492:1501	arg1	determinative					1527:1539	determinative	1527:1539	determinative	1527:1539	It was concluded that the initial composition of the microbiota in the samples was more determinative for incubations with the C. difficile strain than for incubations with the E. coli strain, as the presumed fermentation of prebiotic products showed less influence on the fitness of the C. difficile strain.
34420495	10	62	theme	prebiotic	1664:1672	arg1	products					1674:1681	prebiotic products	1664:1681	prebiotic products	1664:1681	It was concluded that the initial composition of the microbiota in the samples was more determinative for incubations with the C. difficile strain than for incubations with the E. coli strain, as the presumed fermentation of prebiotic products showed less influence on the fitness of the C. difficile strain.
34420495	6	63	from	fitness	918:924	arg1	farms					956:960	some farms	951:960	some farms	951:960	The prebiotic fructo-oligosaccharides (FOS) and its combination with probiotics reduced the residual fitness of the E. coli strain in some farms.
34420495	1	64	theme	probiotics	209:218	arg1	combinations					184:195	potential tailor-made combinations	162:195	potential tailor-made combinations of pre- and probiotics for individual farms	162:239	A novel rapid ex vivo assay was developed as part of a concept to determine potential tailor-made combinations of pre- and probiotics for individual farms.
34420495	8	65	theme	pre-	1097:1100	arg1	combinations					1081:1092	combinations	1081:1092	combinations of pre- and probiotics	1081:1115	Generally, combinations of pre- and probiotics did not show additive effects on fitness for E. coli but displayed farm dependent differences.
34420495	8	66	from	effects	1139:1145	arg1	fitness					1150:1156	fitness	1150:1156	fitness	1150:1156	Generally, combinations of pre- and probiotics did not show additive effects on fitness for E. coli but displayed farm dependent differences.
34420495	5	67	dep	Clostridium	785:795	arg1	perfringens					797:807	perfringens	797:807	perfringens	797:807	The background growth could be inhibited for an Escherichia coli- and a Clostridium difficile- but not for a Clostridium perfringens strain.
34420495	7	68	theme	strain	1062:1067	arg1	fitness					1039:1045	the fitness	1035:1045	the fitness of the E. coli strain	1035:1067	However, notable exceptions occurred in other farms where FOS increased the fitness of the E. coli strain.
34420495	13	69	from	terms	2190:2194	arg1	refinement					2155:2164	refinement	2155:2164	refinement of the ex vivo assay in terms of on-farm processing of samples and validation of unambiguous growth for pathogenic strains from individual farms	2155:2309	Also, refinement of the ex vivo assay in terms of on-farm processing of samples and validation of unambiguous growth for pathogenic strains from individual farms should be addressed.
34420495	1	70	theme	individual	224:233	arg1	farms					235:239	individual farms	224:239	individual farms	224:239	A novel rapid ex vivo assay was developed as part of a concept to determine potential tailor-made combinations of pre- and probiotics for individual farms.
34420495	8	71	theme	probiotics	1106:1115	arg1	combinations					1081:1092	combinations	1081:1092	combinations of pre- and probiotics	1081:1115	Generally, combinations of pre- and probiotics did not show additive effects on fitness for E. coli but displayed farm dependent differences.
34420495	11	72	theme	pathogenic	1800:1809	arg1	strains					1811:1817	both pathogenic strains	1795:1817	both pathogenic strains	1795:1817	Farm dependent differences were pronounced for both pathogenic strains and therefore, this novel screening method offers a promising approach for pre-selecting pre- and probiotics for individual farms.
34420495	7	73	theme	E.	1054:1055	arg1	strain					1062:1067	the E. coli strain	1050:1067	the E. coli strain	1050:1067	However, notable exceptions occurred in other farms where FOS increased the fitness of the E. coli strain.
34420495	13	74	from	assay	2181:2185	arg1	terms					2190:2194	terms	2190:2194	terms of on-farm processing of samples and validation of unambiguous growth	2190:2264	Also, refinement of the ex vivo assay in terms of on-farm processing of samples and validation of unambiguous growth for pathogenic strains from individual farms should be addressed.
34420495	2	75	theme	Sow	242:244	arg1	slurries					253:260	Sow faecal slurries	242:260	Sow faecal slurries from 20 German pig farms	242:285	Sow faecal slurries from 20 German pig farms were anaerobically incubated with pre- and probiotics or their combinations together with pathogenic strains that are of interest in pig production.
34420495	13	76	theme	unambiguous	2247:2257	arg1	growth					2259:2264	unambiguous growth	2247:2264	unambiguous growth	2247:2264	Also, refinement of the ex vivo assay in terms of on-farm processing of samples and validation of unambiguous growth for pathogenic strains from individual farms should be addressed.
34420495	10	77	theme	C.	1727:1728	arg1	strain					1740:1745	the C. difficile strain	1723:1745	the C. difficile strain	1723:1745	It was concluded that the initial composition of the microbiota in the samples was more determinative for incubations with the C. difficile strain than for incubations with the E. coli strain, as the presumed fermentation of prebiotic products showed less influence on the fitness of the C. difficile strain.
34420495	9	78	theme	combination	1396:1406	arg1	application					1360:1370	single application	1353:1370	single application of prebiotics and their combination with probiotics	1353:1422	The effects of pre- and probiotics on the residual fitness of the C. difficile strain were less pronounced, but distinct differences between single application of prebiotics and their combination with probiotics were observed.
34420495	8	79	theme	additive	1130:1137	arg1	effects					1139:1145	additive effects	1130:1145	additive effects on fitness for E. coli	1130:1168	Generally, combinations of pre- and probiotics did not show additive effects on fitness for E. coli but displayed farm dependent differences.
34420495	8	80	theme	dependent	1189:1197	arg1	differences					1199:1209	farm dependent differences	1184:1209	farm dependent differences	1184:1209	Generally, combinations of pre- and probiotics did not show additive effects on fitness for E. coli but displayed farm dependent differences.
34420495	3	81	theme	only	534:537	arg1	growth					539:544	only growth	534:544	only growth of the specific pathogen	534:569	Aliquots of these slurries were then incubated with media containing antibiotic mixtures allowing only growth of the specific pathogen.
34420495	13	82	theme	individual	2294:2303	arg1	farms					2305:2309	individual farms	2294:2309	individual farms	2294:2309	Also, refinement of the ex vivo assay in terms of on-farm processing of samples and validation of unambiguous growth for pathogenic strains from individual farms should be addressed.
34420495	13	83	theme	samples	2221:2227	arg1	validation					2233:2242	validation	2233:2242	validation of unambiguous growth	2233:2264	Also, refinement of the ex vivo assay in terms of on-farm processing of samples and validation of unambiguous growth for pathogenic strains from individual farms should be addressed.
34420495	13	83	theme	samples	2221:2227	arg1	processing					2207:2216	on-farm processing	2199:2216	on-farm processing of samples	2199:2227	Also, refinement of the ex vivo assay in terms of on-farm processing of samples and validation of unambiguous growth for pathogenic strains from individual farms should be addressed.
34420495	5	84	theme	background	680:689	arg1	growth					691:696	The background growth	676:696	The background growth	676:696	The background growth could be inhibited for an Escherichia coli- and a Clostridium difficile- but not for a Clostridium perfringens strain.
34420495	4	85	theme	residual	632:639	arg1	fitness					641:647	the residual fitness	628:647	the residual fitness of the pathogenic strains	628:673	Growth was monitored and lag time was used to determine the residual fitness of the pathogenic strains.
34420495	1	86	theme	potential	162:170	arg1	combinations					184:195	potential tailor-made combinations	162:195	potential tailor-made combinations of pre- and probiotics for individual farms	162:239	A novel rapid ex vivo assay was developed as part of a concept to determine potential tailor-made combinations of pre- and probiotics for individual farms.
34420495	13	87	theme	pathogenic	2270:2279	arg1	strains					2281:2287	pathogenic strains	2270:2287	pathogenic strains from individual farms	2270:2309	Also, refinement of the ex vivo assay in terms of on-farm processing of samples and validation of unambiguous growth for pathogenic strains from individual farms should be addressed.
34420495	10	88	theme	difficile	1569:1577	arg1	strain					1579:1584	the C. difficile strain	1562:1584	the C. difficile strain	1562:1584	It was concluded that the initial composition of the microbiota in the samples was more determinative for incubations with the C. difficile strain than for incubations with the E. coli strain, as the presumed fermentation of prebiotic products showed less influence on the fitness of the C. difficile strain.
34420495	10	89	theme	strain	1740:1745	arg1	fitness					1712:1718	the fitness	1708:1718	the fitness of the C. difficile strain	1708:1745	It was concluded that the initial composition of the microbiota in the samples was more determinative for incubations with the C. difficile strain than for incubations with the E. coli strain, as the presumed fermentation of prebiotic products showed less influence on the fitness of the C. difficile strain.
34420495	12	90	dep	metadata	1978:1985	arg1	management					2005:2014	management	2005:2014	management	2005:2014	However, evaluation of farm metadata (husbandry, feed, management) will be crucial in future studies to determine a tailor-made solution for combinations of pre- and probiotics for individual farms.
34420495	12	90	dep	metadata	1978:1985	arg1	feed					1999:2002	feed	1999:2002	feed	1999:2002	However, evaluation of farm metadata (husbandry, feed, management) will be crucial in future studies to determine a tailor-made solution for combinations of pre- and probiotics for individual farms.
34420495	12	90	dep	metadata	1978:1985	arg1	husbandry					1988:1996	husbandry	1988:1996	husbandry	1988:1996	However, evaluation of farm metadata (husbandry, feed, management) will be crucial in future studies to determine a tailor-made solution for combinations of pre- and probiotics for individual farms.
34420495	1	91	theme	novel	88:92	arg1	part					131:134	part	131:134	part of a concept to determine potential tailor-made combinations of pre- and probiotics for individual farms	131:239	A novel rapid ex vivo assay was developed as part of a concept to determine potential tailor-made combinations of pre- and probiotics for individual farms.
34420495	1	91	theme	novel	88:92	arg1	assay					108:112	A novel rapid ex vivo assay	86:112	A novel rapid ex vivo assay	86:112	A novel rapid ex vivo assay was developed as part of a concept to determine potential tailor-made combinations of pre- and probiotics for individual farms.
34420495	7	92	theme	notable	972:978	arg1	exceptions					980:989	notable exceptions	972:989	notable exceptions	972:989	However, notable exceptions occurred in other farms where FOS increased the fitness of the E. coli strain.
34420495	1	93	theme	ex	100:101	arg1	part					131:134	part	131:134	part of a concept to determine potential tailor-made combinations of pre- and probiotics for individual farms	131:239	A novel rapid ex vivo assay was developed as part of a concept to determine potential tailor-made combinations of pre- and probiotics for individual farms.
34420495	1	93	theme	ex	100:101	arg1	assay					108:112	A novel rapid ex vivo assay	86:112	A novel rapid ex vivo assay	86:112	A novel rapid ex vivo assay was developed as part of a concept to determine potential tailor-made combinations of pre- and probiotics for individual farms.
34420495	0	94	theme	pre-	57:60	arg1	probiotics					66:75	farm specific pre- and probiotics	43:75	farm specific pre- and probiotics	43:75	Novel ex vivo screening assay to preselect farm specific pre- and probiotics in pigs.
34420495	4	95	theme	pathogenic	656:665	arg1	strains					667:673	the pathogenic strains	652:673	the pathogenic strains	652:673	Growth was monitored and lag time was used to determine the residual fitness of the pathogenic strains.
34420495	9	96	theme	residual	1254:1261	arg1	fitness					1263:1269	the residual fitness	1250:1269	the residual fitness of the C. difficile strain	1250:1296	The effects of pre- and probiotics on the residual fitness of the C. difficile strain were less pronounced, but distinct differences between single application of prebiotics and their combination with probiotics were observed.
34420495	6	97	with	fructo-oligosaccharides	831:853	arg1	probiotics					886:895	probiotics	886:895	probiotics	886:895	The prebiotic fructo-oligosaccharides (FOS) and its combination with probiotics reduced the residual fitness of the E. coli strain in some farms.
34420495	0	98	theme	ex	6:7	arg1	assay					24:28	Novel ex vivo screening assay	0:28	Novel ex vivo screening assay	0:28	Novel ex vivo screening assay to preselect farm specific pre- and probiotics in pigs.
34420495	12	99	theme	metadata	1978:1985	arg1	evaluation					1959:1968	evaluation	1959:1968	evaluation of farm metadata (husbandry, feed, management)	1959:2015	However, evaluation of farm metadata (husbandry, feed, management) will be crucial in future studies to determine a tailor-made solution for combinations of pre- and probiotics for individual farms.
34420495	11	100	theme	screening	1845:1853	arg1	method					1855:1860	this novel screening method	1834:1860	this novel screening method	1834:1860	Farm dependent differences were pronounced for both pathogenic strains and therefore, this novel screening method offers a promising approach for pre-selecting pre- and probiotics for individual farms.
34420495	0	101	theme	screening	14:22	arg1	assay					24:28	Novel ex vivo screening assay	0:28	Novel ex vivo screening assay	0:28	Novel ex vivo screening assay to preselect farm specific pre- and probiotics in pigs.
34420495	9	102	theme	C.	1278:1279	arg1	strain					1291:1296	the C. difficile strain	1274:1296	the C. difficile strain	1274:1296	The effects of pre- and probiotics on the residual fitness of the C. difficile strain were less pronounced, but distinct differences between single application of prebiotics and their combination with probiotics were observed.
34420495	0	103	dep	pre-	57:60	arg1	specific					48:55	specific	48:55	specific	48:55	Novel ex vivo screening assay to preselect farm specific pre- and probiotics in pigs.
34420495	12	104	theme	tailor-made	2066:2076	arg1	solution					2078:2085	a tailor-made solution	2064:2085	a tailor-made solution for combinations of pre- and probiotics for individual farms	2064:2146	However, evaluation of farm metadata (husbandry, feed, management) will be crucial in future studies to determine a tailor-made solution for combinations of pre- and probiotics for individual farms.
34420495	9	105	theme	strain	1291:1296	arg1	fitness					1263:1269	the residual fitness	1250:1269	the residual fitness of the C. difficile strain	1250:1296	The effects of pre- and probiotics on the residual fitness of the C. difficile strain were less pronounced, but distinct differences between single application of prebiotics and their combination with probiotics were observed.
34420495	13	106	theme	validation	2233:2242	arg1	terms					2190:2194	terms	2190:2194	terms of on-farm processing of samples and validation of unambiguous growth	2190:2264	Also, refinement of the ex vivo assay in terms of on-farm processing of samples and validation of unambiguous growth for pathogenic strains from individual farms should be addressed.
34420495	10	107	dep	E.	1616:1617	arg1	coli					1619:1622	coli	1619:1622	coli	1619:1622	It was concluded that the initial composition of the microbiota in the samples was more determinative for incubations with the C. difficile strain than for incubations with the E. coli strain, as the presumed fermentation of prebiotic products showed less influence on the fitness of the C. difficile strain.
34420495	10	108	theme	E.	1616:1617	arg1	strain					1624:1629	the E. coli strain	1612:1629	the E. coli strain	1612:1629	It was concluded that the initial composition of the microbiota in the samples was more determinative for incubations with the C. difficile strain than for incubations with the E. coli strain, as the presumed fermentation of prebiotic products showed less influence on the fitness of the C. difficile strain.
34420495	0	109	theme	farm	43:46	arg1	probiotics					66:75	farm specific pre- and probiotics	43:75	farm specific pre- and probiotics	43:75	Novel ex vivo screening assay to preselect farm specific pre- and probiotics in pigs.
34420495	6	110	theme	prebiotic	821:829	arg1	fructo-oligosaccharides					831:853	The prebiotic fructo-oligosaccharides	817:853	The prebiotic fructo-oligosaccharides (FOS)	817:859	The prebiotic fructo-oligosaccharides (FOS) and its combination with probiotics reduced the residual fitness of the E. coli strain in some farms.
34420495	6	110	theme	prebiotic	821:829	arg1	FOS					856:858	FOS	856:858	FOS	856:858	The prebiotic fructo-oligosaccharides (FOS) and its combination with probiotics reduced the residual fitness of the E. coli strain in some farms.
34420495	2	111	theme	pig	420:422	arg1	production					424:433	pig production	420:433	pig production	420:433	Sow faecal slurries from 20 German pig farms were anaerobically incubated with pre- and probiotics or their combinations together with pathogenic strains that are of interest in pig production.
34420495	10	112	theme	presumed	1639:1646	arg1	fermentation					1648:1659	the presumed fermentation	1635:1659	the presumed fermentation of prebiotic products	1635:1681	It was concluded that the initial composition of the microbiota in the samples was more determinative for incubations with the C. difficile strain than for incubations with the E. coli strain, as the presumed fermentation of prebiotic products showed less influence on the fitness of the C. difficile strain.
34420495	10	113	from	samples	1510:1516	arg1	composition					1473:1483	the initial composition	1461:1483	the initial composition of the microbiota in the samples	1461:1516	It was concluded that the initial composition of the microbiota in the samples was more determinative for incubations with the C. difficile strain than for incubations with the E. coli strain, as the presumed fermentation of prebiotic products showed less influence on the fitness of the C. difficile strain.
34420495	10	113	from	samples	1510:1516	arg1	determinative					1527:1539	determinative	1527:1539	determinative	1527:1539	It was concluded that the initial composition of the microbiota in the samples was more determinative for incubations with the C. difficile strain than for incubations with the E. coli strain, as the presumed fermentation of prebiotic products showed less influence on the fitness of the C. difficile strain.
34420495	9	114	theme	distinct	1324:1331	arg1	differences					1333:1343	distinct differences	1324:1343	distinct differences between single application of prebiotics and their combination with probiotics	1324:1422	The effects of pre- and probiotics on the residual fitness of the C. difficile strain were less pronounced, but distinct differences between single application of prebiotics and their combination with probiotics were observed.
34420495	9	115	from	effects	1216:1222	arg1	fitness					1263:1269	the residual fitness	1250:1269	the residual fitness of the C. difficile strain	1250:1296	The effects of pre- and probiotics on the residual fitness of the C. difficile strain were less pronounced, but distinct differences between single application of prebiotics and their combination with probiotics were observed.
34420495	0	116	dep	ex	6:7	arg1	vivo					9:12	vivo	9:12	vivo	9:12	Novel ex vivo screening assay to preselect farm specific pre- and probiotics in pigs.
34420495	4	117	theme	lag	597:599	arg1	time					601:604	lag time	597:604	lag time	597:604	Growth was monitored and lag time was used to determine the residual fitness of the pathogenic strains.
34420495	7	118	dep	E.	1054:1055	arg1	coli					1057:1060	coli	1057:1060	coli	1057:1060	However, notable exceptions occurred in other farms where FOS increased the fitness of the E. coli strain.
34420495	10	119	theme	products	1674:1681	arg1	fermentation					1648:1659	the presumed fermentation	1635:1659	the presumed fermentation of prebiotic products	1635:1681	It was concluded that the initial composition of the microbiota in the samples was more determinative for incubations with the C. difficile strain than for incubations with the E. coli strain, as the presumed fermentation of prebiotic products showed less influence on the fitness of the C. difficile strain.
31958556	0	0	theme	flour	80:84	arg1	properties					27:36	properties	27:36	properties	27:36	Characteristics of pasting properties and morphology changes of rice starch and flour under different heating modes.
31958556	0	0	theme	flour	80:84	arg1	changes					53:59	morphology changes	42:59	morphology changes	42:59	Characteristics of pasting properties and morphology changes of rice starch and flour under different heating modes.
31958556	1	1	theme	starch	146:151	arg1	behavior					129:136	The pasting behavior	117:136	The pasting behavior of rice starch	117:151	The pasting behavior of rice starch and its relationship with cooking properties of rice have been extensively studied.
31958556	1	1	theme	starch	146:151	arg1	relationship					161:172	its relationship	157:172	its relationship with cooking properties of rice	157:204	The pasting behavior of rice starch and its relationship with cooking properties of rice have been extensively studied.
31958556	7	2	theme	rice	1174:1177	arg1	samples					1185:1191	The rice flour samples	1170:1191	The rice flour samples	1170:1191	The rice flour samples showed different morphologies after heating at different modes due to varied amylose content and crystallinity, which contributed to different pasting behavior.
31958556	3	3	theme	types	470:474	arg1	flours					450:455	seven rice flours	439:455	seven rice flours	439:455	In this study, three typical rice starches and seven rice flours of different types and varieties were used to evaluate the effect of cooking modes on their pasting behaviors.
31958556	3	3	theme	types	470:474	arg1	types					470:474	different types	460:474	different types	460:474	In this study, three typical rice starches and seven rice flours of different types and varieties were used to evaluate the effect of cooking modes on their pasting behaviors.
31958556	3	3	theme	types	470:474	arg1	starches					426:433	three typical rice starches	407:433	three typical rice starches	407:433	In this study, three typical rice starches and seven rice flours of different types and varieties were used to evaluate the effect of cooking modes on their pasting behaviors.
31958556	3	3	theme	types	470:474	arg1	varieties					480:488	varieties	480:488	varieties	480:488	In this study, three typical rice starches and seven rice flours of different types and varieties were used to evaluate the effect of cooking modes on their pasting behaviors.
31958556	6	4	theme	temperature	1064:1074	arg1	heating					1086:1092	high temperature long time heating	1059:1092	high temperature long time heating	1059:1092	The morphological changes of rice samples at 95 °C and 120 °C confirmed that high temperature long time heating caused extending of molecules, which exhibited layered structure at 120 °C.
31958556	8	5	theme	rice	1432:1435	arg1	cooking					1437:1443	rice cooking	1432:1443	rice cooking	1432:1443	These results provide useful information for developing strategies to control rice cooking and improve eating quality.
31958556	0	6	theme	different	92:100	arg1	modes					110:114	different heating modes	92:114	different heating modes	92:114	Characteristics of pasting properties and morphology changes of rice starch and flour under different heating modes.
31958556	7	7	theme	different	1326:1334	arg1	behavior					1344:1351	different pasting behavior	1326:1351	different pasting behavior	1326:1351	The rice flour samples showed different morphologies after heating at different modes due to varied amylose content and crystallinity, which contributed to different pasting behavior.
31958556	7	8	theme	pasting	1336:1342	arg1	behavior					1344:1351	different pasting behavior	1326:1351	different pasting behavior	1326:1351	The rice flour samples showed different morphologies after heating at different modes due to varied amylose content and crystallinity, which contributed to different pasting behavior.
31958556	2	9	theme	starch	276:281	arg1	changes					260:266	the viscosity changes	246:266	the viscosity changes of rice starch and flour under conventional cooking mode and high temperature and high pressure (HTHP) mode	246:374	However, the viscosity changes of rice starch and flour under conventional cooking mode and high temperature and high pressure (HTHP) mode remain unknown.
31958556	4	10	theme	different	710:718	arg1	behaviors					728:736	the different pasting behaviors	706:736	the different pasting behaviors of the rice samples	706:756	A detailed discussion about the relationships among chemical composition, thermal properties, and crystallinity were conducted to explain the different pasting behaviors of the rice samples.
31958556	2	11	theme	conventional	299:310	arg1	mode					320:323	conventional cooking mode	299:323	conventional cooking mode	299:323	However, the viscosity changes of rice starch and flour under conventional cooking mode and high temperature and high pressure (HTHP) mode remain unknown.
31958556	5	12	theme	different	925:933	arg1	levels					940:945	different HTHP levels	925:945	different HTHP levels	925:945	The pasting behavior of rice starch was found to be similar with rice flour under standard and conventional heating modes, while remarkably different when treated at different HTHP levels, especially for sticky rice flour.
31958556	7	13	theme	different	1200:1208	arg1	morphologies					1210:1221	different morphologies	1200:1221	different morphologies	1200:1221	The rice flour samples showed different morphologies after heating at different modes due to varied amylose content and crystallinity, which contributed to different pasting behavior.
31958556	2	14	theme	rice	271:274	arg1	starch					276:281	rice starch	271:281	rice starch	271:281	However, the viscosity changes of rice starch and flour under conventional cooking mode and high temperature and high pressure (HTHP) mode remain unknown.
31958556	3	15	theme	rice	445:448	arg1	flours					450:455	seven rice flours	439:455	seven rice flours	439:455	In this study, three typical rice starches and seven rice flours of different types and varieties were used to evaluate the effect of cooking modes on their pasting behaviors.
31958556	5	16	theme	heating	867:873	arg1	modes					875:879	standard and conventional heating modes	841:879	standard and conventional heating modes	841:879	The pasting behavior of rice starch was found to be similar with rice flour under standard and conventional heating modes, while remarkably different when treated at different HTHP levels, especially for sticky rice flour.
31958556	6	17	from	95 °C	1027:1031	arg1	changes					1000:1006	The morphological changes	982:1006	The morphological changes of rice samples at 95 °C and 120 °C	982:1042	The morphological changes of rice samples at 95 °C and 120 °C confirmed that high temperature long time heating caused extending of molecules, which exhibited layered structure at 120 °C.
31958556	6	17	from	95 °C	1027:1031	arg1	samples					1016:1022	rice samples	1011:1022	rice samples at 95 °C and 120 °C	1011:1042	The morphological changes of rice samples at 95 °C and 120 °C confirmed that high temperature long time heating caused extending of molecules, which exhibited layered structure at 120 °C.
31958556	4	18	theme	rice	745:748	arg1	samples					750:756	the rice samples	741:756	the rice samples	741:756	A detailed discussion about the relationships among chemical composition, thermal properties, and crystallinity were conducted to explain the different pasting behaviors of the rice samples.
31958556	3	19	used	used	495:498	arg2	varieties					480:488	varieties	480:488	varieties	480:488	In this study, three typical rice starches and seven rice flours of different types and varieties were used to evaluate the effect of cooking modes on their pasting behaviors.
31958556	3	19	used	used	495:498	arg2	types					470:474	different types	460:474	different types	460:474	In this study, three typical rice starches and seven rice flours of different types and varieties were used to evaluate the effect of cooking modes on their pasting behaviors.
31958556	3	19	used	used	495:498	arg2	starches					426:433	three typical rice starches	407:433	three typical rice starches	407:433	In this study, three typical rice starches and seven rice flours of different types and varieties were used to evaluate the effect of cooking modes on their pasting behaviors.
31958556	3	19	used	used	495:498	arg2	flours					450:455	seven rice flours	439:455	seven rice flours	439:455	In this study, three typical rice starches and seven rice flours of different types and varieties were used to evaluate the effect of cooking modes on their pasting behaviors.
31958556	0	20	theme	heating	102:108	arg1	modes					110:114	different heating modes	92:114	different heating modes	92:114	Characteristics of pasting properties and morphology changes of rice starch and flour under different heating modes.
31958556	5	21	theme	conventional	854:865	arg1	modes					875:879	standard and conventional heating modes	841:879	standard and conventional heating modes	841:879	The pasting behavior of rice starch was found to be similar with rice flour under standard and conventional heating modes, while remarkably different when treated at different HTHP levels, especially for sticky rice flour.
31958556	1	22	with	behavior	129:136	arg1	properties					187:196	cooking properties	179:196	cooking properties of rice	179:204	The pasting behavior of rice starch and its relationship with cooking properties of rice have been extensively studied.
31958556	6	23	from	changes	1000:1006	arg1	120 °C					1037:1042	120 °C	1037:1042	120 °C	1037:1042	The morphological changes of rice samples at 95 °C and 120 °C confirmed that high temperature long time heating caused extending of molecules, which exhibited layered structure at 120 °C.
31958556	6	23	from	changes	1000:1006	arg1	95 °C					1027:1031	95 °C	1027:1031	95 °C	1027:1031	The morphological changes of rice samples at 95 °C and 120 °C confirmed that high temperature long time heating caused extending of molecules, which exhibited layered structure at 120 °C.
31958556	6	24	dep	caused	1094:1099	arg1	exhibited					1131:1139	exhibited	1131:1139	exhibited layered structure at 120 °C	1131:1167	The morphological changes of rice samples at 95 °C and 120 °C confirmed that high temperature long time heating caused extending of molecules, which exhibited layered structure at 120 °C.
31958556	7	25	theme	amylose	1270:1276	arg1	content					1278:1284	varied amylose content	1263:1284	varied amylose content	1263:1284	The rice flour samples showed different morphologies after heating at different modes due to varied amylose content and crystallinity, which contributed to different pasting behavior.
31958556	7	26	theme	flour	1179:1183	arg1	samples					1185:1191	The rice flour samples	1170:1191	The rice flour samples	1170:1191	The rice flour samples showed different morphologies after heating at different modes due to varied amylose content and crystallinity, which contributed to different pasting behavior.
31958556	2	27	dep	mode	320:323	arg1	mode					371:374	mode	371:374	mode	371:374	However, the viscosity changes of rice starch and flour under conventional cooking mode and high temperature and high pressure (HTHP) mode remain unknown.
31958556	5	28	theme	rice	824:827	arg1	flour					829:833	rice flour	824:833	rice flour under standard and conventional heating modes	824:879	The pasting behavior of rice starch was found to be similar with rice flour under standard and conventional heating modes, while remarkably different when treated at different HTHP levels, especially for sticky rice flour.
31958556	3	29	theme	cooking	526:532	arg1	modes					534:538	cooking modes	526:538	cooking modes	526:538	In this study, three typical rice starches and seven rice flours of different types and varieties were used to evaluate the effect of cooking modes on their pasting behaviors.
31958556	1	30	theme	cooking	179:185	arg1	properties					187:196	cooking properties	179:196	cooking properties of rice	179:204	The pasting behavior of rice starch and its relationship with cooking properties of rice have been extensively studied.
31958556	5	31	theme	rice	783:786	arg1	starch					788:793	rice starch	783:793	rice starch	783:793	The pasting behavior of rice starch was found to be similar with rice flour under standard and conventional heating modes, while remarkably different when treated at different HTHP levels, especially for sticky rice flour.
31958556	8	32	theme	useful	1376:1381	arg1	information					1383:1393	useful information	1376:1393	useful information for developing strategies to control rice cooking and improve eating quality	1376:1470	These results provide useful information for developing strategies to control rice cooking and improve eating quality.
31958556	2	33	theme	flour	287:291	arg1	changes					260:266	the viscosity changes	246:266	the viscosity changes of rice starch and flour under conventional cooking mode and high temperature and high pressure (HTHP) mode	246:374	However, the viscosity changes of rice starch and flour under conventional cooking mode and high temperature and high pressure (HTHP) mode remain unknown.
31958556	6	34	theme	rice	1011:1014	arg1	samples					1016:1022	rice samples	1011:1022	rice samples at 95 °C and 120 °C	1011:1042	The morphological changes of rice samples at 95 °C and 120 °C confirmed that high temperature long time heating caused extending of molecules, which exhibited layered structure at 120 °C.
31958556	5	35	theme	starch	788:793	arg1	similar					811:817	similar	811:817	similar	811:817	The pasting behavior of rice starch was found to be similar with rice flour under standard and conventional heating modes, while remarkably different when treated at different HTHP levels, especially for sticky rice flour.
31958556	5	35	theme	starch	788:793	arg1	behavior					771:778	The pasting behavior	759:778	The pasting behavior of rice starch	759:793	The pasting behavior of rice starch was found to be similar with rice flour under standard and conventional heating modes, while remarkably different when treated at different HTHP levels, especially for sticky rice flour.
31958556	5	36	theme	HTHP	935:938	arg1	levels					940:945	different HTHP levels	925:945	different HTHP levels	925:945	The pasting behavior of rice starch was found to be similar with rice flour under standard and conventional heating modes, while remarkably different when treated at different HTHP levels, especially for sticky rice flour.
31958556	6	37	theme	layered	1141:1147	arg1	structure					1149:1157	layered structure	1141:1157	layered structure	1141:1157	The morphological changes of rice samples at 95 °C and 120 °C confirmed that high temperature long time heating caused extending of molecules, which exhibited layered structure at 120 °C.
31958556	4	38	theme	pasting	720:726	arg1	behaviors					728:736	the different pasting behaviors	706:736	the different pasting behaviors of the rice samples	706:756	A detailed discussion about the relationships among chemical composition, thermal properties, and crystallinity were conducted to explain the different pasting behaviors of the rice samples.
31958556	6	39	theme	high	1059:1062	arg1	temperature					1064:1074	high temperature	1059:1074	high temperature long time heating	1059:1092	The morphological changes of rice samples at 95 °C and 120 °C confirmed that high temperature long time heating caused extending of molecules, which exhibited layered structure at 120 °C.
31958556	0	40	theme	morphology	42:51	arg1	changes					53:59	morphology changes	42:59	morphology changes	42:59	Characteristics of pasting properties and morphology changes of rice starch and flour under different heating modes.
31958556	3	41	theme	typical	413:419	arg1	starches					426:433	three typical rice starches	407:433	three typical rice starches	407:433	In this study, three typical rice starches and seven rice flours of different types and varieties were used to evaluate the effect of cooking modes on their pasting behaviors.
31958556	5	42	theme	rice	970:973	arg1	flour					975:979	sticky rice flour	963:979	sticky rice flour	963:979	The pasting behavior of rice starch was found to be similar with rice flour under standard and conventional heating modes, while remarkably different when treated at different HTHP levels, especially for sticky rice flour.
31958556	6	43	theme	samples	1016:1022	arg1	changes					1000:1006	The morphological changes	982:1006	The morphological changes of rice samples at 95 °C and 120 °C	982:1042	The morphological changes of rice samples at 95 °C and 120 °C confirmed that high temperature long time heating caused extending of molecules, which exhibited layered structure at 120 °C.
31958556	1	44	theme	rice	201:204	arg1	properties					187:196	cooking properties	179:196	cooking properties of rice	179:204	The pasting behavior of rice starch and its relationship with cooking properties of rice have been extensively studied.
31958556	4	45	theme	chemical	620:627	arg1	composition					629:639	chemical composition	620:639	chemical composition	620:639	A detailed discussion about the relationships among chemical composition, thermal properties, and crystallinity were conducted to explain the different pasting behaviors of the rice samples.
31958556	3	46	theme	pasting	549:555	arg1	behaviors					557:565	their pasting behaviors	543:565	their pasting behaviors	543:565	In this study, three typical rice starches and seven rice flours of different types and varieties were used to evaluate the effect of cooking modes on their pasting behaviors.
31958556	3	47	theme	rice	421:424	arg1	starches					426:433	three typical rice starches	407:433	three typical rice starches	407:433	In this study, three typical rice starches and seven rice flours of different types and varieties were used to evaluate the effect of cooking modes on their pasting behaviors.
31958556	3	48	theme	different	460:468	arg1	types					470:474	different types	460:474	different types	460:474	In this study, three typical rice starches and seven rice flours of different types and varieties were used to evaluate the effect of cooking modes on their pasting behaviors.
31958556	3	49	theme	modes	534:538	arg1	effect					516:521	the effect	512:521	the effect of cooking modes on their pasting behaviors	512:565	In this study, three typical rice starches and seven rice flours of different types and varieties were used to evaluate the effect of cooking modes on their pasting behaviors.
31958556	2	50	theme	high	329:332	arg1	HTHP					365:368	HTHP	365:368	HTHP	365:368	However, the viscosity changes of rice starch and flour under conventional cooking mode and high temperature and high pressure (HTHP) mode remain unknown.
31958556	2	50	theme	high	329:332	arg1	temperature					334:344	high temperature	329:344	high temperature	329:344	However, the viscosity changes of rice starch and flour under conventional cooking mode and high temperature and high pressure (HTHP) mode remain unknown.
31958556	5	51	theme	pasting	763:769	arg1	similar					811:817	similar	811:817	similar	811:817	The pasting behavior of rice starch was found to be similar with rice flour under standard and conventional heating modes, while remarkably different when treated at different HTHP levels, especially for sticky rice flour.
31958556	5	51	theme	pasting	763:769	arg1	behavior					771:778	The pasting behavior	759:778	The pasting behavior of rice starch	759:793	The pasting behavior of rice starch was found to be similar with rice flour under standard and conventional heating modes, while remarkably different when treated at different HTHP levels, especially for sticky rice flour.
31958556	8	52	theme	eating	1457:1462	arg1	quality					1464:1470	eating quality	1457:1470	eating quality	1457:1470	These results provide useful information for developing strategies to control rice cooking and improve eating quality.
31958556	1	53	theme	rice	141:144	arg1	starch					146:151	rice starch	141:151	rice starch	141:151	The pasting behavior of rice starch and its relationship with cooking properties of rice have been extensively studied.
31958556	7	54	theme	different	1240:1248	arg1	modes					1250:1254	different modes	1240:1254	different modes	1240:1254	The rice flour samples showed different morphologies after heating at different modes due to varied amylose content and crystallinity, which contributed to different pasting behavior.
31958556	6	55	theme	time	1081:1084	arg1	heating					1086:1092	high temperature long time heating	1059:1092	high temperature long time heating	1059:1092	The morphological changes of rice samples at 95 °C and 120 °C confirmed that high temperature long time heating caused extending of molecules, which exhibited layered structure at 120 °C.
31958556	5	56	with	similar	811:817	arg1	flour					829:833	rice flour	824:833	rice flour under standard and conventional heating modes	824:879	The pasting behavior of rice starch was found to be similar with rice flour under standard and conventional heating modes, while remarkably different when treated at different HTHP levels, especially for sticky rice flour.
31958556	2	57	theme	viscosity	250:258	arg1	changes					260:266	the viscosity changes	246:266	the viscosity changes of rice starch and flour under conventional cooking mode and high temperature and high pressure (HTHP) mode	246:374	However, the viscosity changes of rice starch and flour under conventional cooking mode and high temperature and high pressure (HTHP) mode remain unknown.
31958556	4	58	theme	thermal	642:648	arg1	properties					650:659	thermal properties	642:659	thermal properties	642:659	A detailed discussion about the relationships among chemical composition, thermal properties, and crystallinity were conducted to explain the different pasting behaviors of the rice samples.
31958556	1	59	theme	pasting	121:127	arg1	behavior					129:136	The pasting behavior	117:136	The pasting behavior of rice starch	117:151	The pasting behavior of rice starch and its relationship with cooking properties of rice have been extensively studied.
31958556	3	60	from	effect	516:521	arg1	behaviors					557:565	their pasting behaviors	543:565	their pasting behaviors	543:565	In this study, three typical rice starches and seven rice flours of different types and varieties were used to evaluate the effect of cooking modes on their pasting behaviors.
31958556	1	61	with	relationship	161:172	arg1	properties					187:196	cooking properties	179:196	cooking properties of rice	179:204	The pasting behavior of rice starch and its relationship with cooking properties of rice have been extensively studied.
31958556	4	62	theme	samples	750:756	arg1	behaviors					728:736	the different pasting behaviors	706:736	the different pasting behaviors of the rice samples	706:756	A detailed discussion about the relationships among chemical composition, thermal properties, and crystallinity were conducted to explain the different pasting behaviors of the rice samples.
31958556	2	63	theme	cooking	312:318	arg1	mode					320:323	conventional cooking mode	299:323	conventional cooking mode	299:323	However, the viscosity changes of rice starch and flour under conventional cooking mode and high temperature and high pressure (HTHP) mode remain unknown.
31958556	6	64	theme	long	1076:1079	arg1	heating					1086:1092	high temperature long time heating	1059:1092	high temperature long time heating	1059:1092	The morphological changes of rice samples at 95 °C and 120 °C confirmed that high temperature long time heating caused extending of molecules, which exhibited layered structure at 120 °C.
31958556	0	65	theme	starch	69:74	arg1	properties					27:36	properties	27:36	properties	27:36	Characteristics of pasting properties and morphology changes of rice starch and flour under different heating modes.
31958556	0	65	theme	starch	69:74	arg1	changes					53:59	morphology changes	42:59	morphology changes	42:59	Characteristics of pasting properties and morphology changes of rice starch and flour under different heating modes.
31958556	6	66	theme	morphological	986:998	arg1	changes					1000:1006	The morphological changes	982:1006	The morphological changes of rice samples at 95 °C and 120 °C	982:1042	The morphological changes of rice samples at 95 °C and 120 °C confirmed that high temperature long time heating caused extending of molecules, which exhibited layered structure at 120 °C.
31958556	6	67	from	120 °C	1037:1042	arg1	changes					1000:1006	The morphological changes	982:1006	The morphological changes of rice samples at 95 °C and 120 °C	982:1042	The morphological changes of rice samples at 95 °C and 120 °C confirmed that high temperature long time heating caused extending of molecules, which exhibited layered structure at 120 °C.
31958556	6	67	from	120 °C	1037:1042	arg1	samples					1016:1022	rice samples	1011:1022	rice samples at 95 °C and 120 °C	1011:1042	The morphological changes of rice samples at 95 °C and 120 °C confirmed that high temperature long time heating caused extending of molecules, which exhibited layered structure at 120 °C.
31958556	5	68	theme	standard	841:848	arg1	modes					875:879	standard and conventional heating modes	841:879	standard and conventional heating modes	841:879	The pasting behavior of rice starch was found to be similar with rice flour under standard and conventional heating modes, while remarkably different when treated at different HTHP levels, especially for sticky rice flour.
31958556	7	69	theme	varied	1263:1268	arg1	content					1278:1284	varied amylose content	1263:1284	varied amylose content	1263:1284	The rice flour samples showed different morphologies after heating at different modes due to varied amylose content and crystallinity, which contributed to different pasting behavior.
31958556	4	70	theme	detailed	570:577	arg1	discussion					579:588	A detailed discussion	568:588	A detailed discussion about the relationships among chemical composition, thermal properties, and crystallinity	568:678	A detailed discussion about the relationships among chemical composition, thermal properties, and crystallinity were conducted to explain the different pasting behaviors of the rice samples.
31958556	0	71	theme	rice	64:67	arg1	starch					69:74	rice starch	64:74	rice starch	64:74	Characteristics of pasting properties and morphology changes of rice starch and flour under different heating modes.
31958556	5	72	theme	sticky	963:968	arg1	flour					975:979	sticky rice flour	963:979	sticky rice flour	963:979	The pasting behavior of rice starch was found to be similar with rice flour under standard and conventional heating modes, while remarkably different when treated at different HTHP levels, especially for sticky rice flour.
31958556	2	73	theme	high	350:353	arg1	pressure					355:362	high pressure	350:362	high pressure	350:362	However, the viscosity changes of rice starch and flour under conventional cooking mode and high temperature and high pressure (HTHP) mode remain unknown.
31981138	12	0	theme	Lydex	1700:1704	arg1	speed					1691:1695	The degradation speed	1675:1695	The degradation speed of Lydex	1675:1704	CONCLUSIONS The degradation speed of Lydex could be controlled according to the compositions.
31981138	5	1	dep	loaded	700:705	arg1	n = 11					802:807	n = 11	802:807	n = 11	802:807	Lydex 1, 2 and 3 were loaded at the retrosternal space of rabbits in allocated groups before sternal closure, respectively (n = 11 for each group).
31981138	10	2	theme	faster	1213:1218	arg1	degradation					1220:1230	faster degradation	1213:1230	faster degradation (Lydex 2 or Lydex 3)	1213:1251	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	10	3	dep	P = 0.0005	1471:1480	arg1	Lydex					1483:1487	Lydex	1483:1487	Lydex	1483:1487	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	10	3	dep	P = 0.0005	1471:1480	arg1	Lydex					1494:1498	Lydex	1494:1498	Lydex	1494:1498	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	11	4	from	infiltrations	1551:1563	arg1	Lydex					1583:1587	Lydex	1583:1587	Lydex	1583:1587	Retrosternal infiltrations of macrophages in Lydex 1 and Lydex 3 groups are not higher compared to that in unloaded control.
31981138	11	4	from	infiltrations	1551:1563	arg1	Lydex					1595:1599	Lydex	1595:1599	Lydex	1595:1599	Retrosternal infiltrations of macrophages in Lydex 1 and Lydex 3 groups are not higher compared to that in unloaded control.
31981138	3	5	theme	preventative	464:475	arg1	effect					477:482	the preventative effect	460:482	the preventative effect of Lydex on retrosternal adhesion and the relationship between degradation speed and the progression of retrosternal fibrosis	460:608	In the present study, we evaluated the preventative effect of Lydex on retrosternal adhesion and the relationship between degradation speed and the progression of retrosternal fibrosis.
31981138	10	6	theme	Lydex	1345:1349	arg1	fibrosis					1294:1301	retrosternal fibrosis	1281:1301	retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)]	1281:1535	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	10	6	theme	Lydex	1345:1349	arg1	ratio					1364:1368	Lydex 1) [fibrosis ratio	1345:1368	Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3	1345:1410	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	11	7	theme	Retrosternal	1538:1549	arg1	infiltrations					1551:1563	Retrosternal infiltrations	1538:1563	Retrosternal infiltrations of macrophages in Lydex 1 and Lydex 3 groups	1538:1608	Retrosternal infiltrations of macrophages in Lydex 1 and Lydex 3 groups are not higher compared to that in unloaded control.
31981138	8	8	theme	Lydex	1028:1032	arg1	groups					1034:1039	All Lydex groups	1024:1039	All Lydex groups	1024:1039	RESULTS All Lydex groups exhibited less retrosternal adhesion at 4 weeks after loading compared to unloaded control.
31981138	4	9	dep	METHODS	611:617	arg1	rabbits					634:640	Japanese white rabbits	619:640	METHODS Japanese white rabbits	611:640	METHODS Japanese white rabbits are subjected to median sternotomy.
31981138	2	10	theme	Lydex	292:296	arg1	"					297:297	A biodegradable glue "Lydex"	270:297	A biodegradable glue "Lydex"	270:297	A biodegradable glue "Lydex" is composed of food additives, dextran and ε-poly (L-lysine), and the degradation speed can be controlled by the composition.
31981138	13	11	theme	retrosternal	1807:1818	arg1	fibrosis					1820:1827	retrosternal fibrosis	1807:1827	retrosternal fibrosis	1807:1827	The degradation speed affected the progression of retrosternal fibrosis.
31981138	11	12	from	Lydex	1595:1599	arg1	infiltrations					1551:1563	Retrosternal infiltrations	1538:1563	Retrosternal infiltrations of macrophages in Lydex 1 and Lydex 3 groups	1538:1608	Retrosternal infiltrations of macrophages in Lydex 1 and Lydex 3 groups are not higher compared to that in unloaded control.
31981138	3	13	from	effect	477:482	arg1	adhesion					509:516	retrosternal adhesion	496:516	retrosternal adhesion	496:516	In the present study, we evaluated the preventative effect of Lydex on retrosternal adhesion and the relationship between degradation speed and the progression of retrosternal fibrosis.
31981138	3	13	from	effect	477:482	arg1	relationship					526:537	the relationship	522:537	the relationship between degradation speed and the progression of retrosternal fibrosis	522:608	In the present study, we evaluated the preventative effect of Lydex on retrosternal adhesion and the relationship between degradation speed and the progression of retrosternal fibrosis.
31981138	8	14	dep	RESULTS	1016:1022	arg1	exhibited					1041:1049	exhibited	1041:1049	exhibited less retrosternal adhesion at 4 weeks after loading compared to unloaded control	1041:1130	RESULTS All Lydex groups exhibited less retrosternal adhesion at 4 weeks after loading compared to unloaded control.
31981138	7	15	theme	fibrosis	927:934	arg1	Retainment					893:902	Retainment	893:902	Retainment of Lydex, retrosternal fibrosis and the infiltration of macrophages	893:970	Retainment of Lydex, retrosternal fibrosis and the infiltration of macrophages are histologically evaluated, respectively.
31981138	2	16	theme	glue	286:289	arg1	"					297:297	A biodegradable glue "Lydex"	270:297	A biodegradable glue "Lydex"	270:297	A biodegradable glue "Lydex" is composed of food additives, dextran and ε-poly (L-lysine), and the degradation speed can be controlled by the composition.
31981138	13	17	theme	degradation	1761:1771	arg1	speed					1773:1777	The degradation speed	1757:1777	The degradation speed	1757:1777	The degradation speed affected the progression of retrosternal fibrosis.
31981138	1	18	theme	Retrosternal	164:175	arg1	adhesion					177:184	OBJECTIVE Retrosternal adhesion	154:184	OBJECTIVE Retrosternal adhesion after median sternotomy possibly	154:217	OBJECTIVE Retrosternal adhesion after median sternotomy possibly raises the risk of cardiac injury at resternotomy.
31981138	11	19	theme	macrophages	1568:1578	arg1	infiltrations					1551:1563	Retrosternal infiltrations	1538:1563	Retrosternal infiltrations of macrophages in Lydex 1 and Lydex 3 groups	1538:1608	Retrosternal infiltrations of macrophages in Lydex 1 and Lydex 3 groups are not higher compared to that in unloaded control.
31981138	5	20	theme	rabbits	736:742	arg1	space					727:731	the retrosternal space	710:731	the retrosternal space of rabbits	710:742	Lydex 1, 2 and 3 were loaded at the retrosternal space of rabbits in allocated groups before sternal closure, respectively (n = 11 for each group).
31981138	7	21	theme	Lydex	907:911	arg1	Retainment					893:902	Retainment	893:902	Retainment of Lydex, retrosternal fibrosis and the infiltration of macrophages	893:970	Retainment of Lydex, retrosternal fibrosis and the infiltration of macrophages are histologically evaluated, respectively.
31981138	2	22	theme	biodegradable	272:284	arg1	"					297:297	A biodegradable glue "Lydex"	270:297	A biodegradable glue "Lydex"	270:297	A biodegradable glue "Lydex" is composed of food additives, dextran and ε-poly (L-lysine), and the degradation speed can be controlled by the composition.
31981138	10	23	theme	lower	1260:1264	arg1	progression					1266:1276	lower progression	1260:1276	lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)]	1260:1535	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	7	24	theme	retrosternal	914:925	arg1	fibrosis					927:934	retrosternal fibrosis	914:934	retrosternal fibrosis	914:934	Retainment of Lydex, retrosternal fibrosis and the infiltration of macrophages are histologically evaluated, respectively.
31981138	6	25	theme	Retrosternal	826:837	arg1	adhesion					839:846	Retrosternal adhesion	826:846	Retrosternal adhesion	826:846	Retrosternal adhesion was macroscopically evaluated after surgery.
31981138	8	26	theme	retrosternal	1056:1067	arg1	adhesion					1069:1076	less retrosternal adhesion	1051:1076	less retrosternal adhesion	1051:1076	RESULTS All Lydex groups exhibited less retrosternal adhesion at 4 weeks after loading compared to unloaded control.
31981138	10	27	dep	degradation	1220:1230	arg1	Lydex					1233:1237	Lydex	1233:1237	Lydex	1233:1237	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	10	27	dep	degradation	1220:1230	arg1	Lydex					1244:1248	Lydex	1244:1248	Lydex	1244:1248	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	10	28	with	Lydex	1202:1206	arg1	degradation					1220:1230	faster degradation	1213:1230	faster degradation (Lydex 2 or Lydex 3)	1213:1251	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	5	29	theme	sternal	771:777	arg1	closure					779:785	sternal closure	771:785	sternal closure	771:785	Lydex 1, 2 and 3 were loaded at the retrosternal space of rabbits in allocated groups before sternal closure, respectively (n = 11 for each group).
31981138	1	30	theme	median	192:197	arg1	sternotomy					199:208	median sternotomy	192:208	median sternotomy possibly	192:217	OBJECTIVE Retrosternal adhesion after median sternotomy possibly raises the risk of cardiac injury at resternotomy.
31981138	0	31	theme	adhesion	27:34	arg1	Management					0:9	Management	0:9	Management of retrosternal adhesion after median sternotomy by controlling degradation speed of a dextran and ε-poly (L-lysine)-based biocompatible glue.	0:152	Management of retrosternal adhesion after median sternotomy by controlling degradation speed of a dextran and ε-poly (L-lysine)-based biocompatible glue.
31981138	10	32	theme	[fibrosis	1354:1362	arg1	fibrosis					1294:1301	retrosternal fibrosis	1281:1301	retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)]	1281:1535	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	10	32	theme	[fibrosis	1354:1362	arg1	ratio					1364:1368	Lydex 1) [fibrosis ratio	1345:1368	Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3	1345:1410	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	0	33	theme	retrosternal	14:25	arg1	adhesion					27:34	retrosternal adhesion	14:34	retrosternal adhesion	14:34	Management of retrosternal adhesion after median sternotomy by controlling degradation speed of a dextran and ε-poly (L-lysine)-based biocompatible glue.
31981138	3	34	theme	Lydex	487:491	arg1	effect					477:482	the preventative effect	460:482	the preventative effect of Lydex on retrosternal adhesion and the relationship between degradation speed and the progression of retrosternal fibrosis	460:608	In the present study, we evaluated the preventative effect of Lydex on retrosternal adhesion and the relationship between degradation speed and the progression of retrosternal fibrosis.
31981138	1	35	theme	OBJECTIVE	154:162	arg1	adhesion					177:184	OBJECTIVE Retrosternal adhesion	154:184	OBJECTIVE Retrosternal adhesion after median sternotomy possibly	154:217	OBJECTIVE Retrosternal adhesion after median sternotomy possibly raises the risk of cardiac injury at resternotomy.
31981138	10	36	theme	fibrosis	1294:1301	arg1	progression					1266:1276	lower progression	1260:1276	lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)]	1260:1535	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	3	37	theme	present	432:438	arg1	study					440:444	the present study	428:444	the present study	428:444	In the present study, we evaluated the preventative effect of Lydex on retrosternal adhesion and the relationship between degradation speed and the progression of retrosternal fibrosis.
31981138	0	38	theme	median	42:47	arg1	sternotomy					49:58	median sternotomy	42:58	median sternotomy by controlling degradation speed of a dextran and ε-poly (L-lysine)-based biocompatible glue	42:151	Management of retrosternal adhesion after median sternotomy by controlling degradation speed of a dextran and ε-poly (L-lysine)-based biocompatible glue.
31981138	11	39	from	macrophages	1568:1578	arg1	Lydex					1583:1587	Lydex	1583:1587	Lydex	1583:1587	Retrosternal infiltrations of macrophages in Lydex 1 and Lydex 3 groups are not higher compared to that in unloaded control.
31981138	11	39	from	macrophages	1568:1578	arg1	Lydex					1595:1599	Lydex	1595:1599	Lydex	1595:1599	Retrosternal infiltrations of macrophages in Lydex 1 and Lydex 3 groups are not higher compared to that in unloaded control.
31981138	0	40	theme	-based	127:132	arg1	glue					148:151	a dextran and ε-poly (L-lysine)-based biocompatible glue	96:151	a dextran and ε-poly (L-lysine)-based biocompatible glue	96:151	Management of retrosternal adhesion after median sternotomy by controlling degradation speed of a dextran and ε-poly (L-lysine)-based biocompatible glue.
31981138	10	41	theme	retrosternal	1281:1292	arg1	fibrosis					1294:1301	retrosternal fibrosis	1281:1301	retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)]	1281:1535	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	10	41	theme	retrosternal	1281:1292	arg1	ratio					1364:1368	Lydex 1) [fibrosis ratio	1345:1368	Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3	1345:1410	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	4	42	theme	white	628:632	arg1	rabbits					634:640	Japanese white rabbits	619:640	METHODS Japanese white rabbits	611:640	METHODS Japanese white rabbits are subjected to median sternotomy.
31981138	1	43	from	resternotomy	256:267	arg1	risk					230:233	the risk	226:233	the risk of cardiac injury at resternotomy	226:267	OBJECTIVE Retrosternal adhesion after median sternotomy possibly raises the risk of cardiac injury at resternotomy.
31981138	12	44	dep	CONCLUSIONS	1663:1673	arg1	controlled					1715:1724	controlled	1715:1724	could be controlled according to the compositions	1706:1754	CONCLUSIONS The degradation speed of Lydex could be controlled according to the compositions.
31981138	11	45	from	Lydex	1583:1587	arg1	infiltrations					1551:1563	Retrosternal infiltrations	1538:1563	Retrosternal infiltrations of macrophages in Lydex 1 and Lydex 3 groups	1538:1608	Retrosternal infiltrations of macrophages in Lydex 1 and Lydex 3 groups are not higher compared to that in unloaded control.
31981138	4	46	theme	Japanese	619:626	arg1	rabbits					634:640	Japanese white rabbits	619:640	METHODS Japanese white rabbits	611:640	METHODS Japanese white rabbits are subjected to median sternotomy.
31981138	5	47	theme	allocated	747:755	arg1	groups					757:762	allocated groups	747:762	allocated groups	747:762	Lydex 1, 2 and 3 were loaded at the retrosternal space of rabbits in allocated groups before sternal closure, respectively (n = 11 for each group).
31981138	0	48	theme	glue	148:151	arg1	speed					87:91	degradation speed	75:91	degradation speed of a dextran and ε-poly (L-lysine)-based biocompatible glue	75:151	Management of retrosternal adhesion after median sternotomy by controlling degradation speed of a dextran and ε-poly (L-lysine)-based biocompatible glue.
31981138	3	49	theme	degradation	547:557	arg1	speed					559:563	degradation speed	547:563	degradation speed	547:563	In the present study, we evaluated the preventative effect of Lydex on retrosternal adhesion and the relationship between degradation speed and the progression of retrosternal fibrosis.
31981138	10	50	theme	slower	1325:1330	arg1	degradation					1332:1342	slower degradation	1325:1342	slower degradation	1325:1342	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	10	51	dep	P = 0.0005	1504:1513	arg1	Lydex					1516:1520	Lydex	1516:1520	Lydex	1516:1520	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	10	51	dep	P = 0.0005	1504:1513	arg1	Lydex					1527:1531	Lydex	1527:1531	Lydex	1527:1531	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	11	52	dep	Lydex	1583:1587	arg1	groups					1603:1608	groups	1603:1608	groups	1603:1608	Retrosternal infiltrations of macrophages in Lydex 1 and Lydex 3 groups are not higher compared to that in unloaded control.
31981138	0	53	theme	biocompatible	134:146	arg1	glue					148:151	a dextran and ε-poly (L-lysine)-based biocompatible glue	96:151	a dextran and ε-poly (L-lysine)-based biocompatible glue	96:151	Management of retrosternal adhesion after median sternotomy by controlling degradation speed of a dextran and ε-poly (L-lysine)-based biocompatible glue.
31981138	13	54	theme	fibrosis	1820:1827	arg1	progression					1792:1802	the progression	1788:1802	the progression of retrosternal fibrosis	1788:1827	The degradation speed affected the progression of retrosternal fibrosis.
31981138	8	55	theme	unloaded	1115:1122	arg1	control					1124:1130	unloaded control	1115:1130	unloaded control	1115:1130	RESULTS All Lydex groups exhibited less retrosternal adhesion at 4 weeks after loading compared to unloaded control.
31981138	3	56	theme	retrosternal	588:599	arg1	fibrosis					601:608	retrosternal fibrosis	588:608	retrosternal fibrosis	588:608	In the present study, we evaluated the preventative effect of Lydex on retrosternal adhesion and the relationship between degradation speed and the progression of retrosternal fibrosis.
31981138	9	57	theme	degradation	1137:1147	arg1	speed					1149:1153	The degradation speed	1133:1153	The degradation speed of Lydex	1133:1162	The degradation speed of Lydex varied according to the compositions.
31981138	9	58	theme	Lydex	1158:1162	arg1	speed					1149:1153	The degradation speed	1133:1153	The degradation speed of Lydex	1133:1162	The degradation speed of Lydex varied according to the compositions.
31981138	10	59	dep	compared	1303:1310	arg1	fibrosis					1294:1301	retrosternal fibrosis	1281:1301	retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)]	1281:1535	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	10	59	dep	compared	1303:1310	arg1	ratio					1364:1368	Lydex 1) [fibrosis ratio	1345:1368	Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3	1345:1410	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	3	60	theme	fibrosis	601:608	arg1	speed					559:563	degradation speed	547:563	degradation speed	547:563	In the present study, we evaluated the preventative effect of Lydex on retrosternal adhesion and the relationship between degradation speed and the progression of retrosternal fibrosis.
31981138	3	60	theme	fibrosis	601:608	arg1	progression					573:583	the progression	569:583	the progression of retrosternal fibrosis	569:608	In the present study, we evaluated the preventative effect of Lydex on retrosternal adhesion and the relationship between degradation speed and the progression of retrosternal fibrosis.
31981138	7	61	theme	macrophages	960:970	arg1	Lydex					907:911	Lydex	907:911	Lydex	907:911	Retainment of Lydex, retrosternal fibrosis and the infiltration of macrophages are histologically evaluated, respectively.
31981138	7	61	theme	macrophages	960:970	arg1	fibrosis					927:934	retrosternal fibrosis	914:934	retrosternal fibrosis	914:934	Retainment of Lydex, retrosternal fibrosis and the infiltration of macrophages are histologically evaluated, respectively.
31981138	7	61	theme	macrophages	960:970	arg1	infiltration					944:955	the infiltration	940:955	the infiltration of macrophages	940:970	Retainment of Lydex, retrosternal fibrosis and the infiltration of macrophages are histologically evaluated, respectively.
31981138	2	62	theme	food	314:317	arg1	ε-poly					342:347	ε-poly	342:347	ε-poly (L-lysine)	342:358	A biodegradable glue "Lydex" is composed of food additives, dextran and ε-poly (L-lysine), and the degradation speed can be controlled by the composition.
31981138	2	62	theme	food	314:317	arg1	dextran					330:336	dextran	330:336	dextran	330:336	A biodegradable glue "Lydex" is composed of food additives, dextran and ε-poly (L-lysine), and the degradation speed can be controlled by the composition.
31981138	2	62	theme	food	314:317	arg1	additives					319:327	food additives	314:327	food additives	314:327	A biodegradable glue "Lydex" is composed of food additives, dextran and ε-poly (L-lysine), and the degradation speed can be controlled by the composition.
31981138	11	63	theme	unloaded	1645:1652	arg1	control					1654:1660	unloaded control	1645:1660	unloaded control	1645:1660	Retrosternal infiltrations of macrophages in Lydex 1 and Lydex 3 groups are not higher compared to that in unloaded control.
31981138	0	64	theme	degradation	75:85	arg1	speed					87:91	degradation speed	75:91	degradation speed of a dextran and ε-poly (L-lysine)-based biocompatible glue	75:151	Management of retrosternal adhesion after median sternotomy by controlling degradation speed of a dextran and ε-poly (L-lysine)-based biocompatible glue.
31981138	12	65	theme	degradation	1679:1689	arg1	speed					1691:1695	The degradation speed	1675:1695	The degradation speed of Lydex	1675:1704	CONCLUSIONS The degradation speed of Lydex could be controlled according to the compositions.
31981138	0	66	dep	-based	127:132	arg1	dextran					98:104	dextran	98:104	dextran	98:104	Management of retrosternal adhesion after median sternotomy by controlling degradation speed of a dextran and ε-poly (L-lysine)-based biocompatible glue.
31981138	0	66	dep	-based	127:132	arg1	ε-poly					110:115	ε-poly	110:115	ε-poly	110:115	Management of retrosternal adhesion after median sternotomy by controlling degradation speed of a dextran and ε-poly (L-lysine)-based biocompatible glue.
31981138	1	67	theme	cardiac	238:244	arg1	injury					246:251	cardiac injury	238:251	cardiac injury	238:251	OBJECTIVE Retrosternal adhesion after median sternotomy possibly raises the risk of cardiac injury at resternotomy.
31981138	5	68	theme	retrosternal	714:725	arg1	space					727:731	the retrosternal space	710:731	the retrosternal space of rabbits	710:742	Lydex 1, 2 and 3 were loaded at the retrosternal space of rabbits in allocated groups before sternal closure, respectively (n = 11 for each group).
31981138	3	69	theme	retrosternal	496:507	arg1	adhesion					509:516	retrosternal adhesion	496:516	retrosternal adhesion	496:516	In the present study, we evaluated the preventative effect of Lydex on retrosternal adhesion and the relationship between degradation speed and the progression of retrosternal fibrosis.
31981138	7	70	theme	infiltration	944:955	arg1	Retainment					893:902	Retainment	893:902	Retainment of Lydex, retrosternal fibrosis and the infiltration of macrophages	893:970	Retainment of Lydex, retrosternal fibrosis and the infiltration of macrophages are histologically evaluated, respectively.
31981138	4	71	theme	median	659:664	arg1	sternotomy					666:675	median sternotomy	659:675	median sternotomy	659:675	METHODS Japanese white rabbits are subjected to median sternotomy.
31981138	10	72	dep	ratio	1364:1368	arg1	control					1371:1377	control	1371:1377	control	1371:1377	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	10	72	dep	ratio	1364:1368	arg1	P = 0.0005					1471:1480	P = 0.0005	1471:1480	P = 0.0005	1471:1480	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	10	72	dep	ratio	1364:1368	arg1	0.18 ± 0.17					1428:1438	0.18 ± 0.17	1428:1438	0.18 ± 0.17	1428:1438	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	10	72	dep	ratio	1364:1368	arg1	P = 0.0005					1504:1513	P = 0.0005	1504:1513	P = 0.0005	1504:1513	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	10	72	dep	ratio	1364:1368	arg1	0.00 ± 0.00					1443:1453	0.00 ± 0.00	1443:1453	0.00 ± 0.00	1443:1453	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	10	72	dep	ratio	1364:1368	arg1	Lydex					1382:1386	Lydex	1382:1386	Lydex	1382:1386	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	10	72	dep	ratio	1364:1368	arg1	0.60 ± 0.15					1413:1423	0.60 ± 0.15	1413:1423	0.60 ± 0.15	1413:1423	Lydex with faster degradation (Lydex 2 or Lydex 3) showed lower progression of retrosternal fibrosis compared to that with slower degradation (Lydex 1) [fibrosis ratio: control vs Lydex 1 vs Lydex 2 vs Lydex 3: 0.60 ± 0.15 vs 0.18 ± 0.17 vs 0.00 ± 0.00 vs 0.00 ± 0.00, P = 0.0005 (Lydex 1 vs Lydex 2), P = 0.0005 (Lydex 1 vs Lydex 3)].
31981138	1	73	theme	injury	246:251	arg1	risk					230:233	the risk	226:233	the risk of cardiac injury at resternotomy	226:267	OBJECTIVE Retrosternal adhesion after median sternotomy possibly raises the risk of cardiac injury at resternotomy.
34634722	0	0	theme	composite	73:81	arg1	cryogel					90:96	a novel calcium silicate hydrate composite starch cryogel	40:96	a novel calcium silicate hydrate composite starch cryogel	40:96	Removal and recovery of phosphate using a novel calcium silicate hydrate composite starch cryogel.
34634722	7	1	theme	chemical	1187:1194	arg1	adsorption					1196:1205	chemical adsorption	1187:1205	chemical adsorption	1187:1205	The calculated activation energy (Ea) of 43.9 kJ/mol indicates chemical adsorption, while a positive change in enthalpy (ΔH0, 19.3 kJ/mol) indicates the endothermic nature of phosphate adsorption.
34634722	1	2	theme	water	148:152	arg1	quality					154:160	water quality	148:160	water quality	148:160	Phosphate is a major pollutant that deteriorates water quality and causes eutrophication, a novel calcium silicate hydrate composite cryogel (Cry-CSH) was thus successfully prepared for phosphate removal and recovery in this work.
34634722	6	3	theme	equilibrium	989:999	arg1	data					1001:1004	Adsorption equilibrium data	978:1004	Adsorption equilibrium data	978:1004	Adsorption equilibrium data were well fit by the Freundlich isotherm model, while kinetic results were well fit by the pseudo second-order model.
34634722	0	4	theme	hydrate	65:71	arg1	cryogel					90:96	a novel calcium silicate hydrate composite starch cryogel	40:96	a novel calcium silicate hydrate composite starch cryogel	40:96	Removal and recovery of phosphate using a novel calcium silicate hydrate composite starch cryogel.
34634722	2	5	theme	silicate	338:345	arg1	hydrate					347:353	Calcium silicate hydrate	330:353	Calcium silicate hydrate (C-S-H)	330:361	Calcium silicate hydrate (C-S-H) was mixed with the gel precursor (7.5% w/w) prepared from native starch and limewater (saturated calcium hydroxide solution as the cross-linker).
34634722	2	5	theme	silicate	338:345	arg1	C-S-H					356:360	C-S-H	356:360	C-S-H	356:360	Calcium silicate hydrate (C-S-H) was mixed with the gel precursor (7.5% w/w) prepared from native starch and limewater (saturated calcium hydroxide solution as the cross-linker).
34634722	9	6	theme	water	1445:1449	arg1	surface					1451:1457	water surface	1445:1457	water surface for at least 105 days without damage	1445:1494	It can float on water surface for at least 105 days without damage, while its phosphate adsorbed form can be biodegraded within 10 days under soil buried conditions.
34634722	6	7	theme	Adsorption	978:987	arg1	data					1001:1004	Adsorption equilibrium data	978:1004	Adsorption equilibrium data	978:1004	Adsorption equilibrium data were well fit by the Freundlich isotherm model, while kinetic results were well fit by the pseudo second-order model.
34634722	7	8	theme	43.9 kJ/mol	1165:1175	arg1	energy					1150:1155	The calculated activation energy	1124:1155	The calculated activation energy (Ea) of 43.9 kJ/mol	1124:1175	The calculated activation energy (Ea) of 43.9 kJ/mol indicates chemical adsorption, while a positive change in enthalpy (ΔH0, 19.3 kJ/mol) indicates the endothermic nature of phosphate adsorption.
34634722	7	8	theme	43.9 kJ/mol	1165:1175	arg1	Ea					1158:1159	Ea	1158:1159	Ea	1158:1159	The calculated activation energy (Ea) of 43.9 kJ/mol indicates chemical adsorption, while a positive change in enthalpy (ΔH0, 19.3 kJ/mol) indicates the endothermic nature of phosphate adsorption.
34634722	4	9	theme	easier	741:746	arg1	recovery					748:755	an easier recovery	738:755	an easier recovery	738:755	This had C-S-H nanoparticles (75 mg) immobilized on a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery and without the losses that occur when using C-S-H nanoparticles.
34634722	5	10	from	120 min	871:877	arg1	equilibrium					856:866	equilibrium	856:866	equilibrium at 120 min with adsorption capacity of 2.50 mgPO43-/gCry-CSH (65.42 mgPO43-/gC-S-H)	856:950	The phosphate adsorption reaches equilibrium at 120 min with adsorption capacity of 2.50 mgPO43-/gCry-CSH (65.42 mgPO43-/gC-S-H) under optimum conditions.
34634722	7	11	dep	enthalpy	1235:1242	arg1	19.3 kJ/mol					1250:1260	19.3 kJ/mol	1250:1260	19.3 kJ/mol	1250:1260	The calculated activation energy (Ea) of 43.9 kJ/mol indicates chemical adsorption, while a positive change in enthalpy (ΔH0, 19.3 kJ/mol) indicates the endothermic nature of phosphate adsorption.
34634722	7	11	dep	enthalpy	1235:1242	arg1	ΔH0					1245:1247	ΔH0	1245:1247	ΔH0	1245:1247	The calculated activation energy (Ea) of 43.9 kJ/mol indicates chemical adsorption, while a positive change in enthalpy (ΔH0, 19.3 kJ/mol) indicates the endothermic nature of phosphate adsorption.
34634722	0	12	theme	starch	83:88	arg1	cryogel					90:96	a novel calcium silicate hydrate composite starch cryogel	40:96	a novel calcium silicate hydrate composite starch cryogel	40:96	Removal and recovery of phosphate using a novel calcium silicate hydrate composite starch cryogel.
34634722	5	13	with	equilibrium	856:866	arg1	capacity					895:902	adsorption capacity	884:902	adsorption capacity of 2.50 mgPO43-/gCry-CSH (65.42 mgPO43-/gC-S-H)	884:950	The phosphate adsorption reaches equilibrium at 120 min with adsorption capacity of 2.50 mgPO43-/gCry-CSH (65.42 mgPO43-/gC-S-H) under optimum conditions.
34634722	7	14	theme	positive	1216:1223	arg1	change					1225:1230	a positive change	1214:1230	a positive change in enthalpy (ΔH0, 19.3 kJ/mol)	1214:1261	The calculated activation energy (Ea) of 43.9 kJ/mol indicates chemical adsorption, while a positive change in enthalpy (ΔH0, 19.3 kJ/mol) indicates the endothermic nature of phosphate adsorption.
34634722	4	15	theme	cryogel	679:685	arg1	network					687:693	a monolithic floatable cryogel network	656:693	a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery	656:755	This had C-S-H nanoparticles (75 mg) immobilized on a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery and without the losses that occur when using C-S-H nanoparticles.
34634722	4	15	theme	cryogel	679:685	arg1	height					721:726	2.5 cm diameter × 1.0 cm height	696:726	2.5 cm diameter × 1.0 cm height	696:726	This had C-S-H nanoparticles (75 mg) immobilized on a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery and without the losses that occur when using C-S-H nanoparticles.
34634722	10	16	theme	significant	1628:1638	arg1	potential					1640:1648	the significant potential	1624:1648	the significant potential of Cry-CSH for practical and environmentally friendly phosphate removal and recovery	1624:1733	Thus, this work demonstrated the significant potential of Cry-CSH for practical and environmentally friendly phosphate removal and recovery.
34634722	2	17	theme	hydroxide	468:476	arg1	solution					478:485	saturated calcium hydroxide solution	450:485	saturated calcium hydroxide solution as the cross-linker	450:505	Calcium silicate hydrate (C-S-H) was mixed with the gel precursor (7.5% w/w) prepared from native starch and limewater (saturated calcium hydroxide solution as the cross-linker).
34634722	4	18	theme	monolithic	658:667	arg1	network					687:693	a monolithic floatable cryogel network	656:693	a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery	656:755	This had C-S-H nanoparticles (75 mg) immobilized on a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery and without the losses that occur when using C-S-H nanoparticles.
34634722	4	18	theme	monolithic	658:667	arg1	height					721:726	2.5 cm diameter × 1.0 cm height	696:726	2.5 cm diameter × 1.0 cm height	696:726	This had C-S-H nanoparticles (75 mg) immobilized on a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery and without the losses that occur when using C-S-H nanoparticles.
34634722	6	19	theme	Freundlich	1027:1036	arg1	model					1047:1051	the Freundlich isotherm model	1023:1051	the Freundlich isotherm model	1023:1051	Adsorption equilibrium data were well fit by the Freundlich isotherm model, while kinetic results were well fit by the pseudo second-order model.
34634722	10	20	theme	phosphate	1704:1712	arg1	removal					1714:1720	practical and environmentally friendly phosphate removal	1665:1720	practical and environmentally friendly phosphate removal	1665:1720	Thus, this work demonstrated the significant potential of Cry-CSH for practical and environmentally friendly phosphate removal and recovery.
34634722	7	21	theme	endothermic	1277:1287	arg1	nature					1289:1294	the endothermic nature	1273:1294	the endothermic nature of phosphate adsorption	1273:1318	The calculated activation energy (Ea) of 43.9 kJ/mol indicates chemical adsorption, while a positive change in enthalpy (ΔH0, 19.3 kJ/mol) indicates the endothermic nature of phosphate adsorption.
34634722	4	22	theme	C-S-H	613:617	arg1	75 mg					634:638	75 mg	634:638	75 mg	634:638	This had C-S-H nanoparticles (75 mg) immobilized on a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery and without the losses that occur when using C-S-H nanoparticles.
34634722	4	22	theme	C-S-H	613:617	arg1	nanoparticles					619:631	C-S-H nanoparticles	613:631	C-S-H nanoparticles (75 mg)	613:639	This had C-S-H nanoparticles (75 mg) immobilized on a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery and without the losses that occur when using C-S-H nanoparticles.
34634722	2	23	theme	calcium	460:466	arg1	solution					478:485	saturated calcium hydroxide solution	450:485	saturated calcium hydroxide solution as the cross-linker	450:505	Calcium silicate hydrate (C-S-H) was mixed with the gel precursor (7.5% w/w) prepared from native starch and limewater (saturated calcium hydroxide solution as the cross-linker).
34634722	5	24	theme	optimum	958:964	arg1	conditions					966:975	optimum conditions	958:975	optimum conditions	958:975	The phosphate adsorption reaches equilibrium at 120 min with adsorption capacity of 2.50 mgPO43-/gCry-CSH (65.42 mgPO43-/gC-S-H) under optimum conditions.
34634722	7	25	theme	phosphate	1299:1307	arg1	adsorption					1309:1318	phosphate adsorption	1299:1318	phosphate adsorption	1299:1318	The calculated activation energy (Ea) of 43.9 kJ/mol indicates chemical adsorption, while a positive change in enthalpy (ΔH0, 19.3 kJ/mol) indicates the endothermic nature of phosphate adsorption.
34634722	8	26	theme	excellent	1392:1400	arg1	efficiency					1410:1419	excellent removal efficiency	1392:1419	excellent removal efficiency (>98%)	1392:1426	Cry-CSH can remove phosphate from wastewater and effluent samples with excellent removal efficiency (>98%).
34634722	8	26	theme	excellent	1392:1400	arg1	%					1425:1425	>98%	1422:1425	>98%	1422:1425	Cry-CSH can remove phosphate from wastewater and effluent samples with excellent removal efficiency (>98%).
34634722	6	27	theme	kinetic	1060:1066	arg1	results					1068:1074	kinetic results	1060:1074	kinetic results	1060:1074	Adsorption equilibrium data were well fit by the Freundlich isotherm model, while kinetic results were well fit by the pseudo second-order model.
34634722	2	28	theme	saturated	450:458	arg1	solution					478:485	saturated calcium hydroxide solution	450:485	saturated calcium hydroxide solution as the cross-linker	450:505	Calcium silicate hydrate (C-S-H) was mixed with the gel precursor (7.5% w/w) prepared from native starch and limewater (saturated calcium hydroxide solution as the cross-linker).
34634722	9	29	theme	phosphate	1507:1515	arg1	form					1526:1529	its phosphate adsorbed form	1503:1529	its phosphate adsorbed form	1503:1529	It can float on water surface for at least 105 days without damage, while its phosphate adsorbed form can be biodegraded within 10 days under soil buried conditions.
34634722	5	30	theme	phosphate	827:835	arg1	adsorption					837:846	The phosphate adsorption	823:846	The phosphate adsorption	823:846	The phosphate adsorption reaches equilibrium at 120 min with adsorption capacity of 2.50 mgPO43-/gCry-CSH (65.42 mgPO43-/gC-S-H) under optimum conditions.
34634722	2	31	theme	w/w	402:404	arg1	precursor					386:394	the gel precursor	378:394	the gel precursor (7.5% w/w) prepared from native starch and limewater (saturated calcium hydroxide solution as the cross-linker)	378:506	Calcium silicate hydrate (C-S-H) was mixed with the gel precursor (7.5% w/w) prepared from native starch and limewater (saturated calcium hydroxide solution as the cross-linker).
34634722	2	31	theme	w/w	402:404	arg1	%					400:400	7.5% w/w	397:404	7.5% w/w	397:404	Calcium silicate hydrate (C-S-H) was mixed with the gel precursor (7.5% w/w) prepared from native starch and limewater (saturated calcium hydroxide solution as the cross-linker).
34634722	7	32	theme	calculated	1128:1137	arg1	energy					1150:1155	The calculated activation energy	1124:1155	The calculated activation energy (Ea) of 43.9 kJ/mol	1124:1175	The calculated activation energy (Ea) of 43.9 kJ/mol indicates chemical adsorption, while a positive change in enthalpy (ΔH0, 19.3 kJ/mol) indicates the endothermic nature of phosphate adsorption.
34634722	7	32	theme	calculated	1128:1137	arg1	Ea					1158:1159	Ea	1158:1159	Ea	1158:1159	The calculated activation energy (Ea) of 43.9 kJ/mol indicates chemical adsorption, while a positive change in enthalpy (ΔH0, 19.3 kJ/mol) indicates the endothermic nature of phosphate adsorption.
34634722	4	33	theme	C-S-H	802:806	arg1	nanoparticles					808:820	C-S-H nanoparticles	802:820	C-S-H nanoparticles	802:820	This had C-S-H nanoparticles (75 mg) immobilized on a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery and without the losses that occur when using C-S-H nanoparticles.
34634722	5	34	theme	mgPO43-/gCry-CSH	912:927	arg1	capacity					895:902	adsorption capacity	884:902	adsorption capacity of 2.50 mgPO43-/gCry-CSH (65.42 mgPO43-/gC-S-H)	884:950	The phosphate adsorption reaches equilibrium at 120 min with adsorption capacity of 2.50 mgPO43-/gCry-CSH (65.42 mgPO43-/gC-S-H) under optimum conditions.
34634722	7	35	theme	activation	1139:1148	arg1	energy					1150:1155	The calculated activation energy	1124:1155	The calculated activation energy (Ea) of 43.9 kJ/mol	1124:1175	The calculated activation energy (Ea) of 43.9 kJ/mol indicates chemical adsorption, while a positive change in enthalpy (ΔH0, 19.3 kJ/mol) indicates the endothermic nature of phosphate adsorption.
34634722	7	35	theme	activation	1139:1148	arg1	Ea					1158:1159	Ea	1158:1159	Ea	1158:1159	The calculated activation energy (Ea) of 43.9 kJ/mol indicates chemical adsorption, while a positive change in enthalpy (ΔH0, 19.3 kJ/mol) indicates the endothermic nature of phosphate adsorption.
34634722	8	36	with	samples	1379:1385	arg1	efficiency					1410:1419	excellent removal efficiency	1392:1419	excellent removal efficiency (>98%)	1392:1426	Cry-CSH can remove phosphate from wastewater and effluent samples with excellent removal efficiency (>98%).
34634722	8	36	with	samples	1379:1385	arg1	%					1425:1425	>98%	1422:1425	>98%	1422:1425	Cry-CSH can remove phosphate from wastewater and effluent samples with excellent removal efficiency (>98%).
34634722	4	37	theme	diameter × 1.0 cm	703:719	arg1	network					687:693	a monolithic floatable cryogel network	656:693	a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery	656:755	This had C-S-H nanoparticles (75 mg) immobilized on a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery and without the losses that occur when using C-S-H nanoparticles.
34634722	4	37	theme	diameter × 1.0 cm	703:719	arg1	height					721:726	2.5 cm diameter × 1.0 cm height	696:726	2.5 cm diameter × 1.0 cm height	696:726	This had C-S-H nanoparticles (75 mg) immobilized on a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery and without the losses that occur when using C-S-H nanoparticles.
34634722	6	38	theme	isotherm	1038:1045	arg1	model					1047:1051	the Freundlich isotherm model	1023:1051	the Freundlich isotherm model	1023:1051	Adsorption equilibrium data were well fit by the Freundlich isotherm model, while kinetic results were well fit by the pseudo second-order model.
34634722	9	39	theme	adsorbed	1517:1524	arg1	form					1526:1529	its phosphate adsorbed form	1503:1529	its phosphate adsorbed form	1503:1529	It can float on water surface for at least 105 days without damage, while its phosphate adsorbed form can be biodegraded within 10 days under soil buried conditions.
34634722	10	40	theme	environmentally	1679:1693	arg1	removal					1714:1720	practical and environmentally friendly phosphate removal	1665:1720	practical and environmentally friendly phosphate removal	1665:1720	Thus, this work demonstrated the significant potential of Cry-CSH for practical and environmentally friendly phosphate removal and recovery.
34634722	4	41	theme	2.5 cm	696:701	arg1	network					687:693	a monolithic floatable cryogel network	656:693	a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery	656:755	This had C-S-H nanoparticles (75 mg) immobilized on a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery and without the losses that occur when using C-S-H nanoparticles.
34634722	4	41	theme	2.5 cm	696:701	arg1	height					721:726	2.5 cm diameter × 1.0 cm height	696:726	2.5 cm diameter × 1.0 cm height	696:726	This had C-S-H nanoparticles (75 mg) immobilized on a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery and without the losses that occur when using C-S-H nanoparticles.
34634722	1	42	theme	novel	191:195	arg1	cryogel					232:238	a novel calcium silicate hydrate composite cryogel	189:238	a novel calcium silicate hydrate composite cryogel (Cry-CSH)	189:248	Phosphate is a major pollutant that deteriorates water quality and causes eutrophication, a novel calcium silicate hydrate composite cryogel (Cry-CSH) was thus successfully prepared for phosphate removal and recovery in this work.
34634722	1	42	theme	novel	191:195	arg1	Cry-CSH					241:247	Cry-CSH	241:247	Cry-CSH	241:247	Phosphate is a major pollutant that deteriorates water quality and causes eutrophication, a novel calcium silicate hydrate composite cryogel (Cry-CSH) was thus successfully prepared for phosphate removal and recovery in this work.
34634722	1	43	theme	phosphate	285:293	arg1	removal					295:301	phosphate removal	285:301	phosphate removal	285:301	Phosphate is a major pollutant that deteriorates water quality and causes eutrophication, a novel calcium silicate hydrate composite cryogel (Cry-CSH) was thus successfully prepared for phosphate removal and recovery in this work.
34634722	1	44	theme	calcium	197:203	arg1	cryogel					232:238	a novel calcium silicate hydrate composite cryogel	189:238	a novel calcium silicate hydrate composite cryogel (Cry-CSH)	189:248	Phosphate is a major pollutant that deteriorates water quality and causes eutrophication, a novel calcium silicate hydrate composite cryogel (Cry-CSH) was thus successfully prepared for phosphate removal and recovery in this work.
34634722	1	44	theme	calcium	197:203	arg1	Cry-CSH					241:247	Cry-CSH	241:247	Cry-CSH	241:247	Phosphate is a major pollutant that deteriorates water quality and causes eutrophication, a novel calcium silicate hydrate composite cryogel (Cry-CSH) was thus successfully prepared for phosphate removal and recovery in this work.
34634722	8	45	theme	effluent	1370:1377	arg1	samples					1379:1385	wastewater and effluent samples	1355:1385	samples	1379:1385	Cry-CSH can remove phosphate from wastewater and effluent samples with excellent removal efficiency (>98%).
34634722	2	46	theme	gel	382:384	arg1	precursor					386:394	the gel precursor	378:394	the gel precursor (7.5% w/w) prepared from native starch and limewater (saturated calcium hydroxide solution as the cross-linker)	378:506	Calcium silicate hydrate (C-S-H) was mixed with the gel precursor (7.5% w/w) prepared from native starch and limewater (saturated calcium hydroxide solution as the cross-linker).
34634722	2	46	theme	gel	382:384	arg1	%					400:400	7.5% w/w	397:404	7.5% w/w	397:404	Calcium silicate hydrate (C-S-H) was mixed with the gel precursor (7.5% w/w) prepared from native starch and limewater (saturated calcium hydroxide solution as the cross-linker).
34634722	0	47	theme	phosphate	24:32	arg1	recovery					12:19	recovery	12:19	recovery	12:19	Removal and recovery of phosphate using a novel calcium silicate hydrate composite starch cryogel.
34634722	0	47	theme	phosphate	24:32	arg1	Removal					0:6	Removal	0:6	Removal	0:6	Removal and recovery of phosphate using a novel calcium silicate hydrate composite starch cryogel.
34634722	2	48	theme	native	421:426	arg1	starch					428:433	native starch	421:433	native starch	421:433	Calcium silicate hydrate (C-S-H) was mixed with the gel precursor (7.5% w/w) prepared from native starch and limewater (saturated calcium hydroxide solution as the cross-linker).
34634722	3	49	theme	macroporous	581:591	arg1	composite					593:601	an interconnected macroporous composite	563:601	an interconnected macroporous composite	563:601	The mixture was frozen and thawed for 3 cycles giving an interconnected macroporous composite.
34634722	9	50	from	float	1436:1440	arg1	surface					1451:1457	water surface	1445:1457	water surface for at least 105 days without damage	1445:1494	It can float on water surface for at least 105 days without damage, while its phosphate adsorbed form can be biodegraded within 10 days under soil buried conditions.
34634722	1	51	theme	silicate	205:212	arg1	cryogel					232:238	a novel calcium silicate hydrate composite cryogel	189:238	a novel calcium silicate hydrate composite cryogel (Cry-CSH)	189:248	Phosphate is a major pollutant that deteriorates water quality and causes eutrophication, a novel calcium silicate hydrate composite cryogel (Cry-CSH) was thus successfully prepared for phosphate removal and recovery in this work.
34634722	1	51	theme	silicate	205:212	arg1	Cry-CSH					241:247	Cry-CSH	241:247	Cry-CSH	241:247	Phosphate is a major pollutant that deteriorates water quality and causes eutrophication, a novel calcium silicate hydrate composite cryogel (Cry-CSH) was thus successfully prepared for phosphate removal and recovery in this work.
34634722	0	52	theme	novel	42:46	arg1	cryogel					90:96	a novel calcium silicate hydrate composite starch cryogel	40:96	a novel calcium silicate hydrate composite starch cryogel	40:96	Removal and recovery of phosphate using a novel calcium silicate hydrate composite starch cryogel.
34634722	2	53	dep	limewater	439:447	arg1	solution					478:485	saturated calcium hydroxide solution	450:485	saturated calcium hydroxide solution as the cross-linker	450:505	Calcium silicate hydrate (C-S-H) was mixed with the gel precursor (7.5% w/w) prepared from native starch and limewater (saturated calcium hydroxide solution as the cross-linker).
34634722	10	54	theme	Cry-CSH	1653:1659	arg1	potential					1640:1648	the significant potential	1624:1648	the significant potential of Cry-CSH for practical and environmentally friendly phosphate removal and recovery	1624:1733	Thus, this work demonstrated the significant potential of Cry-CSH for practical and environmentally friendly phosphate removal and recovery.
34634722	6	55	theme	pseudo	1097:1102	arg1	model					1117:1121	the pseudo second-order model	1093:1121	the pseudo second-order model	1093:1121	Adsorption equilibrium data were well fit by the Freundlich isotherm model, while kinetic results were well fit by the pseudo second-order model.
34634722	2	56	theme	Calcium	330:336	arg1	hydrate					347:353	Calcium silicate hydrate	330:353	Calcium silicate hydrate (C-S-H)	330:361	Calcium silicate hydrate (C-S-H) was mixed with the gel precursor (7.5% w/w) prepared from native starch and limewater (saturated calcium hydroxide solution as the cross-linker).
34634722	2	56	theme	Calcium	330:336	arg1	C-S-H					356:360	C-S-H	356:360	C-S-H	356:360	Calcium silicate hydrate (C-S-H) was mixed with the gel precursor (7.5% w/w) prepared from native starch and limewater (saturated calcium hydroxide solution as the cross-linker).
34634722	7	57	from	change	1225:1230	arg1	enthalpy					1235:1242	enthalpy	1235:1242	enthalpy (ΔH0, 19.3 kJ/mol)	1235:1261	The calculated activation energy (Ea) of 43.9 kJ/mol indicates chemical adsorption, while a positive change in enthalpy (ΔH0, 19.3 kJ/mol) indicates the endothermic nature of phosphate adsorption.
34634722	9	58	theme	soil	1571:1574	arg1	conditions					1583:1592	soil buried conditions	1571:1592	soil buried conditions	1571:1592	It can float on water surface for at least 105 days without damage, while its phosphate adsorbed form can be biodegraded within 10 days under soil buried conditions.
34634722	5	59	theme	adsorption	884:893	arg1	capacity					895:902	adsorption capacity	884:902	adsorption capacity of 2.50 mgPO43-/gCry-CSH (65.42 mgPO43-/gC-S-H)	884:950	The phosphate adsorption reaches equilibrium at 120 min with adsorption capacity of 2.50 mgPO43-/gCry-CSH (65.42 mgPO43-/gC-S-H) under optimum conditions.
34634722	8	60	theme	removal	1402:1408	arg1	efficiency					1410:1419	excellent removal efficiency	1392:1419	excellent removal efficiency (>98%)	1392:1426	Cry-CSH can remove phosphate from wastewater and effluent samples with excellent removal efficiency (>98%).
34634722	8	60	theme	removal	1402:1408	arg1	%					1425:1425	>98%	1422:1425	>98%	1422:1425	Cry-CSH can remove phosphate from wastewater and effluent samples with excellent removal efficiency (>98%).
34634722	1	61	theme	major	114:118	arg1	pollutant					120:128	a major pollutant	112:128	a major pollutant that deteriorates water quality and causes eutrophication	112:186	Phosphate is a major pollutant that deteriorates water quality and causes eutrophication, a novel calcium silicate hydrate composite cryogel (Cry-CSH) was thus successfully prepared for phosphate removal and recovery in this work.
34634722	1	61	theme	major	114:118	arg1	Phosphate					99:107	Phosphate	99:107	Phosphate	99:107	Phosphate is a major pollutant that deteriorates water quality and causes eutrophication, a novel calcium silicate hydrate composite cryogel (Cry-CSH) was thus successfully prepared for phosphate removal and recovery in this work.
34634722	8	62	theme	wastewater	1355:1364	arg1	samples					1379:1385	wastewater and effluent samples	1355:1385	samples	1379:1385	Cry-CSH can remove phosphate from wastewater and effluent samples with excellent removal efficiency (>98%).
34634722	1	63	theme	hydrate	214:220	arg1	cryogel					232:238	a novel calcium silicate hydrate composite cryogel	189:238	a novel calcium silicate hydrate composite cryogel (Cry-CSH)	189:248	Phosphate is a major pollutant that deteriorates water quality and causes eutrophication, a novel calcium silicate hydrate composite cryogel (Cry-CSH) was thus successfully prepared for phosphate removal and recovery in this work.
34634722	1	63	theme	hydrate	214:220	arg1	Cry-CSH					241:247	Cry-CSH	241:247	Cry-CSH	241:247	Phosphate is a major pollutant that deteriorates water quality and causes eutrophication, a novel calcium silicate hydrate composite cryogel (Cry-CSH) was thus successfully prepared for phosphate removal and recovery in this work.
34634722	10	64	theme	friendly	1695:1702	arg1	removal					1714:1720	practical and environmentally friendly phosphate removal	1665:1720	practical and environmentally friendly phosphate removal	1665:1720	Thus, this work demonstrated the significant potential of Cry-CSH for practical and environmentally friendly phosphate removal and recovery.
34634722	4	65	contain	had	609:611	arg2	75 mg					634:638	75 mg	634:638	75 mg	634:638	This had C-S-H nanoparticles (75 mg) immobilized on a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery and without the losses that occur when using C-S-H nanoparticles.
34634722	4	65	contain	had	609:611	arg1	This					604:607	This	604:607	This	604:607	This had C-S-H nanoparticles (75 mg) immobilized on a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery and without the losses that occur when using C-S-H nanoparticles.
34634722	4	65	contain	had	609:611	arg2	nanoparticles					619:631	C-S-H nanoparticles	613:631	C-S-H nanoparticles (75 mg)	613:639	This had C-S-H nanoparticles (75 mg) immobilized on a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery and without the losses that occur when using C-S-H nanoparticles.
34634722	3	66	theme	interconnected	566:579	arg1	composite					593:601	an interconnected macroporous composite	563:601	an interconnected macroporous composite	563:601	The mixture was frozen and thawed for 3 cycles giving an interconnected macroporous composite.
34634722	4	67	theme	floatable	669:677	arg1	network					687:693	a monolithic floatable cryogel network	656:693	a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery	656:755	This had C-S-H nanoparticles (75 mg) immobilized on a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery and without the losses that occur when using C-S-H nanoparticles.
34634722	4	67	theme	floatable	669:677	arg1	height					721:726	2.5 cm diameter × 1.0 cm height	696:726	2.5 cm diameter × 1.0 cm height	696:726	This had C-S-H nanoparticles (75 mg) immobilized on a monolithic floatable cryogel network (2.5 cm diameter × 1.0 cm height) enabling an easier recovery and without the losses that occur when using C-S-H nanoparticles.
34634722	6	68	theme	second-order	1104:1115	arg1	model					1117:1121	the pseudo second-order model	1093:1121	the pseudo second-order model	1093:1121	Adsorption equilibrium data were well fit by the Freundlich isotherm model, while kinetic results were well fit by the pseudo second-order model.
34634722	1	69	theme	composite	222:230	arg1	cryogel					232:238	a novel calcium silicate hydrate composite cryogel	189:238	a novel calcium silicate hydrate composite cryogel (Cry-CSH)	189:248	Phosphate is a major pollutant that deteriorates water quality and causes eutrophication, a novel calcium silicate hydrate composite cryogel (Cry-CSH) was thus successfully prepared for phosphate removal and recovery in this work.
34634722	1	69	theme	composite	222:230	arg1	Cry-CSH					241:247	Cry-CSH	241:247	Cry-CSH	241:247	Phosphate is a major pollutant that deteriorates water quality and causes eutrophication, a novel calcium silicate hydrate composite cryogel (Cry-CSH) was thus successfully prepared for phosphate removal and recovery in this work.
34634722	0	70	theme	silicate	56:63	arg1	cryogel					90:96	a novel calcium silicate hydrate composite starch cryogel	40:96	a novel calcium silicate hydrate composite starch cryogel	40:96	Removal and recovery of phosphate using a novel calcium silicate hydrate composite starch cryogel.
34634722	7	71	theme	adsorption	1309:1318	arg1	nature					1289:1294	the endothermic nature	1273:1294	the endothermic nature of phosphate adsorption	1273:1318	The calculated activation energy (Ea) of 43.9 kJ/mol indicates chemical adsorption, while a positive change in enthalpy (ΔH0, 19.3 kJ/mol) indicates the endothermic nature of phosphate adsorption.
34634722	10	72	theme	practical	1665:1673	arg1	removal					1714:1720	practical and environmentally friendly phosphate removal	1665:1720	practical and environmentally friendly phosphate removal	1665:1720	Thus, this work demonstrated the significant potential of Cry-CSH for practical and environmentally friendly phosphate removal and recovery.
34634722	0	73	theme	calcium	48:54	arg1	cryogel					90:96	a novel calcium silicate hydrate composite starch cryogel	40:96	a novel calcium silicate hydrate composite starch cryogel	40:96	Removal and recovery of phosphate using a novel calcium silicate hydrate composite starch cryogel.
34634722	9	74	theme	buried	1576:1581	arg1	conditions					1583:1592	soil buried conditions	1571:1592	soil buried conditions	1571:1592	It can float on water surface for at least 105 days without damage, while its phosphate adsorbed form can be biodegraded within 10 days under soil buried conditions.